LOCUS       DASTKS010000001         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_446132, whole genome shotgun sequence.
ACCESSION   DASTKS010000001 DASTKS010000000
VERSION     DASTKS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446132"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..502
                     /locus_tag="VFQ35_00005"
     CDS             170..502
                     /locus_tag="VFQ35_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929031.1"
                     /translation="MEEAFPIFERALREAGTIVPTPRAAALRLGRKTAEKMLKGEISA
                     YEGSSQIWATARRVTNDSVGELDPFIDAASEWEVRPADRPVFEDAMLRAARELTRSVE
                     PNGGGDRE"
     gene            1023..1493
                     /locus_tag="VFQ35_00010"
     CDS             1023..1493
                     /locus_tag="VFQ35_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9929032.1"
                     /translation="MQSVTHLLLATVAAVICSFPGVALAQPATASSVQLGVGFRYGVD
                     VDKAQGDDDPWRAGFGVNGGYTLPNALYLGGNFEYFFGQSYGDDGAGDSAKIWQLTAE
                     AGYDVALAPFAVLRPKLGAGIAKLQYKLCIDLDCERDSESDFTMLPGATPCLIA"
     gene            1730..1921
                     /locus_tag="VFQ35_00015"
     CDS             1730..1921
                     /locus_tag="VFQ35_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929033.1"
                     /translation="MKKYKLLRGSELKLSAPHGAGTISATFKATGPDHYRVVEVSDSD
                     MFKDGLAVAIADGTIVPLA"
     gene            2464..2787
                     /locus_tag="VFQ35_00020"
     CDS             2464..2787
                     /locus_tag="VFQ35_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929034.1"
                     /translation="MATRILCTILLACALAACGGASEPGSPEPAPVESCWHIEPEGYS
                     TTQWVWTCEGHPPPTPPLCSWVDHDDTRVMSCPDELPSGSVPAGCELTDVNVAHYLYT
                     CGAAK"
     gene            2769..2993
                     /locus_tag="VFQ35_00025"
     CDS             2769..2993
                     /locus_tag="VFQ35_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929035.1"
                     /translation="MWGREVSAITERRGQAARAAALLRSAADRVEATAPDEGPEDEAA
                     RYRGAAVLAHTAGLELAFVAGTVAAQEVES"
     gene            2990..3202
                     /locus_tag="VFQ35_00030"
     CDS             2990..3202
                     /locus_tag="VFQ35_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929036.1"
                     /translation="MSRARARKPAPARTREELRRVLEDAWAAAVGVAEELKERHPEAF
                     AHCRLSVRMLTGCLIAIDRAVAELPR"
     gene            3275..4633
                     /locus_tag="VFQ35_00035"
     CDS             3275..4633
                     /locus_tag="VFQ35_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9929037.1"
                     /translation="MPRPRTGTLLPKRKDGFYSAKVWVTRDGKPVRETVDLGTRVKAL
                     AEQRLAELVNGEHAAPPTKREAETFAKAAARINDQREADGVRAHADEWQRLKKWAIPA
                     FGDERIDRVSAAHINSALDAVKKDGLSRQTAAHLLNAIRQTFTGLIREGAIEVNPANL
                     DAVLMPRFPPTTKKERAVLNDDELSAYLRWEHPIPEHRISVLERQCMALLARTFGGLR
                     TGDLHSLRWDGFDLPPAGETEPCAFAWGVAPRQKTRRPQRLAVPTEGRAALWEWWTQT
                     NRPRSGLVFPSRRGDRKGEAKTKVSHAKAFRRDLRRAWGLETWDERKGAFVPAREPTE
                     RERVVLDGDRYTLPVDWHSWRRAYSQALADAGVNAQDASALAGHASLAAHQRYLMRSE
                     KTFEAPAKAMPRLILAVSEVQSVAGTKPADLTDVTPENSNDFSGADGTRTHRRHGSRS
                     VG"
     gene            4623..4874
                     /locus_tag="VFQ35_00040"
     CDS             4623..4874
                     /locus_tag="VFQ35_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929038.1"
                     /translation="MSDSDVWGENPGERDTPSVAPNRDVTGPCSNVVTEEIRGDRVAR
                     DLDRALEGWRVDRDPEGLRRALAGVILLLNAQPGDARGD"
BASE COUNT          879 a         1601 c         1695 g          763 t
ORIGIN      
        1 atcagccgaa ttcgcgaacg ggggaaaggg ggcagtgtcg gcgcccctcc atcgcctggc
       61 ccgtcatggc gctgcttgcc gctcttcgcc cctcaaacgc tccgctcgcg cgcgaagcgc
      121 gcgggcggag cgaaatgtga gtttagttgc ggcgatggac gccccgcgca tggaggaggc
      181 ttttccgatt ttcgaacggg cgctgagaga agcgggaacg atcgtcccca cgccacgcgc
      241 agcggccctg cggctcggga ggaagaccgc cgaaaagatg ttgaagggcg aaatatcagc
      301 ctacgagggg agcagccaga tttgggcgac cgcgcggcgc gtgacaaacg actccgtggg
      361 cgaacttgat ccgttcattg atgcagcgag cgaatgggag gttcgtccag cggatcggcc
      421 cgtttttgaa gacgcgatgt tacgtgcagc gcgggagctg acgcgcagtg ttgagccgaa
      481 cggcggtggg gacagagagt gaagaggccg cggctggggc ggggccagcc gcggccgtgc
      541 gtgcagttgt gggggtggcg ggcgtgaggt gccgaaccgc gtagcacgct cgctgatcga
      601 cgcgcaggcc gataacggcg cgcatccgag catggtttcg ccggcgccga agtgcgctgg
      661 cgccaggaaa tctacgatgt agcgatcagc gaggactact tggcgacgaa agtagacgcc
      721 gaggcgcccg gcagcgatgg ctcgacggag gcggtcttcg ggcgtattga gggaggcgcg
      781 ggacgtgcgc gtcccgcgaa gtccggctcc gcgccggccg cgaggtggtg cgcgcagcgc
      841 taccttgcgt agccgcgagg cttgcgcggt agcgcctccg gcggggaggc tatcgagagc
      901 caagcggacc gtcggcatga acgacaaggg ctggcccgac tcagatcatt gactatgctt
      961 cggcgtcgtc gatgtcgtaa gaagtcagac attcaaagcc aaaagtgcag aacaacgaat
     1021 tgatgcaatc ggtcacccat ctgcttctcg caacggtcgc agccgtaatc tgctcgttcc
     1081 ccggggtagc tctcgcgcag ccggcgaccg ccagctcggt gcagctcggc gtggggttcc
     1141 ggtacggggt cgacgtcgac aaggcccaag gcgacgacga tccgtggcgg gcggggtttg
     1201 gggtgaacgg cggttatacg ctgccgaatg cgctctatct gggcggcaac ttcgagtatt
     1261 tcttcgggca gagttacggt gatgacggcg cgggggatag cgccaagatt tggcagctca
     1321 cggcggaggc gggctacgac gtggcgctcg ctcctttcgc cgtgctgcgt ccgaagctcg
     1381 gcgccggtat cgccaagctt cagtacaagc tttgtatcga cttggattgc gagcgagata
     1441 gcgagagcga tttcacgatg ctcccggggg cgacgccatg cctgatcgct taagggccct
     1501 cgaatgggag agtcgatcga tcaactgatc ttgtccgtcg acttttccga acagaagcgc
     1561 gaagactcgc aacaaatcga tcgagatcgc cattcgcgag ccctatgggt tcgcgcgtcg
     1621 gtgtgagggc gtcttagtga ctttcggcgc cccgggtaat ggtgcccgtt cgacacacgc
     1681 cgacgcgcga cccgccctct ttgatggttg caactgaaag gatccgaccg tgaaaaagta
     1741 caagctcctt cgaggctccg aattgaagct ctccgcgccg catggcgcgg ggacgatctc
     1801 ggcgacgttc aaggcaacgg gccccgacca ctatcgcgtc gtcgaggtct ccgactccga
     1861 catgttcaaa gacgggctag ccgtggcgat tgcggacggg acgatcgtcc ccctcgcgtg
     1921 atcgaatgtc cgcgtccggc ggccggttcg gtcggtctcg tccctctctt gagctctacc
     1981 tcgcacgctg caaagagatc ttggattccg gcgttcaaac gacggaagcc gtgtcccaaa
     2041 tgcggctccg ctgccggcgc cgctgcgagg cggcggggcg acccgttccc gattggtgcg
     2101 ccaaccgagc ggggcaaccg ccgacgccga gaaccgaagc gccacccccg ccgccgccgc
     2161 cgcgcgagcc ggccccgcca ccggtcgcgc tggatccgaa attgagcgag tggcgagcgg
     2221 cgcaccctgg aagcgtcgtc gcggttagtc gctcgggcgt caccctgcac gaatacggcg
     2281 ccgagccgcg aaccttcccg acggtcgagg cggcgctcgc acccgcgctc cctgaacgaa
     2341 tggaactgga ttcgcgttcc attcacaggg cgcccgaccg tggctaattc gagaagaacg
     2401 cccgacgcgc ggacggcaat ccgcgcgccg agctgaccac gaccgaaagg atgagtttcg
     2461 accatggcta cccgaattct atgcacgatc ctgctcgcgt gcgcgctcgc cgcgtgcggc
     2521 ggcgcttccg aacccggctc accggagccc gcgcccgtcg agtcttgctg gcacatcgaa
     2581 cccgaaggct actcaaccac tcagtgggtc tggacgtgcg aagggcaccc gccgcccacg
     2641 cccccgctct gctcgtgggt cgatcacgac gacacgcgcg tcatgtcgtg ccccgacgag
     2701 cttccgtcgg gcagcgtgcc cgcgggctgc gagctcacgg acgtgaacgt cgcgcactac
     2761 ctctacacgt gtggggccgc gaagtgagcg ccatcaccga acgacggggg caggccgccc
     2821 gagcagcggc gctcctacgg tcggccgcgg accgcgtcga agcaaccgcg ccggacgaag
     2881 ggcccgaaga cgaggccgct cgctaccgcg gcgcggccgt gctcgcccac acggcggggc
     2941 tagagcttgc tttcgtcgct ggcaccgtcg ccgcgcaaga ggtcgagtcg tgagccgcgc
     3001 tcgcgctcgg aagccggccc cggcgcgcac ccgggaagag cttcgcaggg tcctcgaaga
     3061 cgcatgggcc gcggccgtgg gcgtcgccga agagctcaaa gaacgtcacc ccgaggcgtt
     3121 cgcacactgc cggttgagcg tccgcatgct tacgggctgc ttgatcgcca tcgatcgcgc
     3181 ggtcgccgag ctcccccggt agaccctttc ccggtgtcgc acgtaggcac cgggattcac
     3241 acccgcgcgt ccgaatgcga cgcttctccg cgccatgcct cgccctcgaa ccggtacgct
     3301 ccttcccaaa cgcaaagacg gcttctactc ggccaaggtt tgggtcacgc gcgacggcaa
     3361 gcccgtgcgc gaaaccgtcg acctcggcac ccgcgtcaag gcgctcgccg agcaacggct
     3421 agccgagctc gtcaacggtg agcacgcggc cccaccgacg aagcgcgagg cggagacgtt
     3481 cgcgaaagcc gccgcacgaa tcaacgacca gcgcgaggcg gacggcgttc gcgcgcacgc
     3541 cgacgaatgg caacggctca agaagtgggc gatcccggcg ttcggtgacg agcggatcga
     3601 tcgcgtgagt gcggcacaca tcaattcggc gctcgacgcc gtgaagaagg acgggctctc
     3661 gcggcagacg gccgcgcacc tcttgaacgc gatccgtcag acgttcacgg gtctcatccg
     3721 cgaaggtgcg atcgaagtga acccggcgaa cctcgacgcg gtgctcatgc cgcgctttcc
     3781 gccgacgacc aagaaggagc gcgccgtcct gaacgacgac gaattgagcg cttatctgcg
     3841 ttgggagcac ccgatcccag agcatcgaat ttccgtgctc gaacgtcagt gcatggcgct
     3901 cctcgctcga acgttcggcg ggcttcgcac aggcgatttg cactcgctcc gatgggacgg
     3961 cttcgacttg ccgcccgcgg gtgagacgga gccgtgcgcg ttcgcgtggg gcgtggcgcc
     4021 gcgtcagaag acgcgccggc cgcaacggct cgccgttccg accgagggcc gtgcggcgct
     4081 ctgggagtgg tggacgcaaa ccaaccgacc acggtccggc ctcgtgttcc cgtcgaggcg
     4141 aggggaccgc aagggcgagg caaagacgaa ggtcagccat gcgaaagcgt tccgccgcga
     4201 cctgcgccga gcgtggggcc tcgagacgtg ggacgagcga aagggggcct tcgtcccggc
     4261 gcgcgaaccg accgagcgcg agcgcgtcgt gctcgacggc gaccgttata cgttgcccgt
     4321 cgactggcac tcgtggcgcc gcgcgtactc gcaggcgctc gccgatgccg gcgtgaacgc
     4381 gcaagacgcg agcgccctcg ccggacacgc gagcctcgcc gcacaccaac gctacttgat
     4441 gcgctccgag aagaccttcg aagcgcctgc gaaggcgatg ccccggctaa ttctcgcggt
     4501 ttctgaggtt cagtccgtgg cggggactaa accagcggat ttgacggacg tgactccgga
     4561 aaactctaac gatttcagcg gggcggacgg gactcgaact caccgaaggc atgggtcgcg
     4621 tagtgtcgga tagtgacgtt tggggcgaaa accccggcga acgcgacacc ccgagcgtcg
     4681 ccccgaatcg cgacgtaacc ggaccttgta gtaatgtagt aaccgaggaa atccgcgggg
     4741 atcgcgtcgc gcgcgatctc gatcgtgcgc tcgaggggtg gcgggtcgac cgcgatccag
     4801 agggactccg acgcgcgctc gcgggggtca ttttgctgct gaacgcccag ccgggcgacg
     4861 cgcgaggcga ctgacacgcc gactggaaag ggacttcgcc ccagcaccga gcgggcttca
     4921 atcgaccggt gctgaggc
//
LOCUS       DASTKS010000002         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_223257, whole genome shotgun sequence.
ACCESSION   DASTKS010000002 DASTKS010000000
VERSION     DASTKS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223257"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFQ35_00045"
     CDS             <1..1285
                     /locus_tag="VFQ35_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929039.1"
                     /translation="AADPALLSRPPPLIEEEPAQRAAIIDAASALVERVARSLNPADL
                     EAEVTPDLAVRIDQLLRECWATLSTDRALGAQLPVERVMAVARAELTDIGPLAELLED
                     PTVSEVAVIGRERLTVTRTGKPALVDVGFSNPLSVRWAVARLCARSAVPLGPATYLER
                     RLWDGTSLTATLTAGSPIVLLERSRKLSGSLEELVRRGMVSRALANFLQQCLLARLNL
                     LVVGPRDGGVELLLGALASSLPESELIYAGRFAPERSSTSASLQLSGVELPEAVGLAA
                     RAPLVRVLCELASSTLTQSVVAAISDGADGVIASRPASSLRRGLLRIVSDLRSRYVEG
                     AAEMLAGAFEIVIEVARLRDDRHRVLRVAEVLGADHEEIDLSDVFSFTIDRTASGGLI
                     EGSFVPASAMPAIAELMRNRGAAIDGTQFTRPSGR"
     gene            complement(1443..1955)
                     /gene="def"
                     /locus_tag="VFQ35_00050"
     CDS             complement(1443..1955)
                     /gene="def"
                     /locus_tag="VFQ35_00050"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET9929040.1"
                     /translation="MLREILKYPDPRLREVAKPVDQVTDELRTLIDDMAETMYASKGC
                     GLAATQIGIDKRIFVVDCAGEDEPSQLMAFINPEIVETDGSQTWDEGCLSFPGVSEEI
                     KRAERVKMRALDRNGKQFEIEADGLLAVALQHELDHLNGVLMIDKLNALKRRMMGRKL
                     AKAKQHEADV"
BASE COUNT          308 a          747 c          696 g          417 t
ORIGIN      
        1 tgcggctgat ccggctttgc tttcgcgacc cccgccgctc atcgaagagg aaccggcgca
       61 gcgtgcggcc atcatcgacg ccgcgtcggc gctcgtcgag cgcgtcgcgc ggtcgctcaa
      121 tcccgctgat ctcgaggcgg aagtcacacc cgatctcgcc gtccgcatcg accagctgct
      181 gcgcgaatgt tgggcgacgc tgagcacgga tcgggcgctc ggagcgcagc tcccggtgga
      241 gcgagtgatg gcggttgctc gcgccgagct cacggacatc ggtccgctcg cggagctgct
      301 cgaagaccca accgtcagcg aagtcgccgt gattgggcgc gagagactga cggtcacgcg
      361 aaccggcaag cccgcgctcg tggacgttgg cttttcgaat ccgctctcgg tgcgctgggc
      421 ggtcgcgcgc ttgtgtgccc gctccgcggt tccgctcggg ccggccacgt atctcgagcg
      481 ccgtctctgg gacggaacgt cgctcaccgc cactctcacc gccggttcgc cgatcgtgct
      541 cctcgagcgt agccgcaagc tgtctggcag cttggaggag ctcgttcgtc gcggcatggt
      601 gtcccgcgcg ctcgcgaact ttcttcagca atgcctgctc gcgcggctca acctgctcgt
      661 cgttggcccc cgtgatggcg gagtcgagct cttgctcggc gcgctggctt cgtcattgcc
      721 ggagagcgaa ctcatctacg ccgggcgctt cgcccccgag cgctcctcga cgtcagcgtc
      781 actgcagctc tctggcgtcg agcttcccga ggcggtcggt ctcgccgccc gcgccccgct
      841 cgtacgcgtt ttgtgcgagc tcgccagctc gaccctcacg cagagcgtcg tcgcggcgat
      901 ctccgacggc gccgacggcg tgattgcgtc gcgacctgcg agctcgctgc gccgcggctt
      961 gctgcgcatc gtttcggacc ttcggtctcg ctacgtcgaa ggcgcggctg aaatgctcgc
     1021 cggcgctttc gagatcgtca tcgaggttgc gcgcctgcgc gacgaccgtc accgagtttt
     1081 gcgggtggcc gaggtgctcg gggccgacca cgaggagatc gacctttccg acgtcttctc
     1141 cttcaccatc gaccgcaccg cttcgggcgg cttgatcgag ggaagcttcg ttcccgcatc
     1201 tgcgatgccg gcgatcgccg agctgatgcg aaatcgaggc gccgcgatcg acggcacgca
     1261 attcactcga ccttccgggc gctgagcgcg gcgacgccgc tctcccgctt cgtcggacac
     1321 ggggctcagt cgaccttccg ggcgccgagc gtgcgcgacg ccgctctccg cttcgtcact
     1381 acaagcgtct gaacgaagcg gctgcgctca gccctcgagt tttgcccact gagctcgcgc
     1441 gttcagacgt cggcttcgtg ctgcttcgct ttggcgagct tgcggcccat catgcggcgc
     1501 ttcaacgcat tcagcttgtc gatcatcagc acgccattga ggtggtcgag ctcgtgctgg
     1561 agcgccacgg cgaggaggcc gtcggcctcg atctcgaatt gctttccgtt gcggtcgagc
     1621 gcgcgcattt ttacccgctc ggcccgcttg atctcttcgc tgacgccggg aaacgacagg
     1681 cacccttcgt cccaagtctg gctcccgtcg gtctcgacga tctccgggtt gatgaaagcc
     1741 atcaactgac tcggctcgtc ttcaccggcg cagtcgacca cgaagatgcg cttgtcgatg
     1801 ccgatctgcg tggcagcgag accgcaaccc ttggaggcgt acatggtttc cgccatgtca
     1861 tcgatcagcg tgcgaagctc gtcggttacc tggtcgacgg gcttggcgac ttcgcgcaat
     1921 cgagggtccg ggtacttcaa aatctcgcgc agcattgcag tcacgccaat ctaacacccg
     1981 gattttggcg tcgcaacgta ggggcaggcg cacaccgggc catgcgcgac tgtccaccag
     2041 gagcgcaacg gctcaaccag ccggattttg acgggtcacg cgcttcggat acacgagcgg
     2101 accccagacg taggcgtcgg taccgcagag ctcccagcgc ggtggctcga tgcgcggcgc
     2161 atcggcgg
//
LOCUS       DASTKS010000003         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1561052, whole genome shotgun sequence.
ACCESSION   DASTKS010000003 DASTKS010000000
VERSION     DASTKS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561052"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFQ35_00055"
     CDS             complement(<1..202)
                     /locus_tag="VFQ35_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HET9929041.1"
                     /translation="MAGQSSAGGPCIYQEPNFPFRATGVAWPHSAEHWRPRHCHRELE
                     LNLVTRGRGRFVLDAGEYELERG"
     gene            complement(277..2682)
                     /locus_tag="VFQ35_00060"
     CDS             complement(277..2682)
                     /locus_tag="VFQ35_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929042.1"
                     /translation="MILEMPTPAIIFWGPEQSQIYNDGYAVIMGPRHPQYFGAPYREC
                     WPDTYPVIYPWMVDVLERGEVKHVENTLFTLTRHGFTEEAYFTFTFSPLRDDAERIAG
                     IFQPVIETTGFVLAERRAATLRAVAAQTGGAASLMSALASNPEDIPFAVLYVEDAAGG
                     IVFAGHVGLLDEQELERATALRVVQRVLDSGQDERLDDVQALIGRPHVGHWSDVTRSA
                     VALAVRVAAGAPAERPLGVLVLGLTPRLRYDESYQSFFHSVAQQVAAQLASGRAATAE
                     RELLEREQTARQEAELHREHLMSLLTQAPTPIVLLRGPSYVIELANPYTLQLWGRTAE
                     QVLGRALFDALPEIRGQVFRNLLDSVYTTGVAHVSETPVVAALDRRGNGELEDRHLTF
                     VYAPLRLATGAIDGILVLAFDVTEQVRAREQVEGLRVAAEAASQAKDEFLAILGHELR
                     NPLAPILTAVDLLRMRSAAAFAKEIDVIDRQANHLVRLVDDLLDVSRITRGKIELRKE
                     RIEVARLVDRAIEMVTPLLEERQHEVNVRVPRTGLVVEVDPSRLAQVLANLLSNAAKY
                     TERRGEVSVSARRAGSDITISVKDTGIGIEPDMLPRVFDLFAQEPQALDRARGGLGLG
                     LAIVRRLMELHQGRVDAHSDGRGRGSEFSIVLPAAATDTVSPSTSASSAVPFDLGRRR
                     RLLIVDDNEDAANLLAESLREFGYDTRVAYDGPSAILVAAQFQPEIALLDIGLPVMDG
                     YELARRLREADAGQLQLVAITGYGQPSDRERALDAGFDAHFAKPVACERLAETLRTLA
                     ATP"
     gene            2941..>3125
                     /locus_tag="VFQ35_00065"
     CDS             2941..>3125
                     /locus_tag="VFQ35_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929043.1"
                     /translation="MASNQRARAVIRCGRFTDDGSRDSSMKLTGASVAEEFCNELCQD
                     RHTPAFGDAARVEPFGL"
BASE COUNT          532 a         1028 c         1018 g          547 t
ORIGIN      
        1 cgccgcgctc gagctcgtat tcgccggcgt cgagcacgaa acggccgcga ccgcgcgtca
       61 ccaagttcag ctcgagctca cgatgacagt gccgcggccg ccagtgttcg gcgctgtgcg
      121 gccaggcaac tcccgtcgcg cgaaatggga agtttggctc ctgatagatg cacggtcccc
      181 cggcgctgct ctggccagcc atctccaatc gatagcaccg gaacgccgtt cgtgtctccc
      241 gcaacgggcc cagttcagct tctggaaccg caaatgtcag ggagtcgcgg ccagcgtgcg
      301 cagcgtctcg gcgagcctct cgcacgccac cggtttcgcg aagtgcgcgt cgaacccggc
      361 gtcgagcgct cgctcgcggt ccgatggttg accgtatccg gtgatcgcca cgagctggag
      421 ttggcccgcg tcggcttcgc gcagtcgccg cgcgagctcg taaccgtcca tcactggaag
      481 cccgatgtcg agtagcgcga tctccggttg gaattgggcg gcaacgagaa tggcgcttgg
      541 gccatcgtac gcgacgcgcg tgtcgtagcc gaactcgcga agtgactccg cgagcaaatt
      601 ggcggcgtcc tcgttgtcgt cgacgatcaa gagccggcgc cgccggccga ggtcgaacgg
      661 aaccgccgag ctcgccgaag tgctcggcga cacggtgtcg gtcgcagccg ccggcaacac
      721 gatgctgaac tcgctgcctc gtcctcgccc gtcgctgtgc gcgtcgacgc ggccctggtg
      781 gagctccatc aatcgacgca cgatcgcaag gccgagcccg agccctccgc gcgcgcggtc
      841 gagcgcttgc ggttcttgtg cgaacaggtc gaacacgcgt ggcagcatgt ccggttcgat
      901 gccaatcccg gtgtctttca cggagatggt gatgtccgac ccagctcggc gcgcgctgac
      961 cgagacctcc ccgcgccgct ccgtgtactt cgccgcgttc gaaagcaggt tcgcgagcac
     1021 ttgagcaagg cgactcggat cgacctcgac gacgagcccg gtgcgaggga cgcgcacgtt
     1081 gacttcgtgc tgccgctctt cgagcagggg agtcaccatt tcgatcgcgc gatcgacgag
     1141 gcgggctact tcgatgcgct ccttacgtag ctcgattttg cccctcgtga tgcgcgagac
     1201 gtcgagcagg tcgtcgacga ggcgtaccag atggttcgcc tgccgatcga tgacgtcgat
     1261 ttctttcgca aaagccgcgg cagagcgcat gcgcagcaag tcgaccgcgg tcaagattgg
     1321 cgcgagcggg tttcgcagct catgcccgag gatcgccaga aattcgtctt tggcctgact
     1381 cgcggcctcg gcggccaccc gcagcccttc gacttgctct cgggcgcgga cttgctccgt
     1441 cacgtcgaac gcaaggacca agatcccgtc gatggcgccg gtggcgagcc ggagcggtgc
     1501 gtacacgaag gtgagatgac ggtcttcgag ctcgccgttg ccacgccggt cgagcgcggc
     1561 gaccaccggc gtctcactga cgtgcgcgac tccggtcgtg tacacgctgt cgagcagatt
     1621 tcggaatacc tggccgcgga tctcgggcag cgcatcgaag agcgctcgcc cgagtacttg
     1681 ctcggccgta cgaccccaga gctgcaacgt gtacgggttc gcgagctcga tgacgtacga
     1741 cgggccgcgc agtagcacta tcggcgtcgg cgcctgagtg agcagcgaca tcaagtgttc
     1801 acgatggagt tcggcctcct ggcgagccgt ctgttcgcgc tcgaggagct cgcgctcggc
     1861 cgtcgcggca cggcccgaag cgagctgcgc cgcgacctgc tgcgccacgg agtgaaagaa
     1921 ggactggtac gactcgtcgt agcggagccg cggcgtgagg ccgagaacca gcacccccaa
     1981 ggggcgctcc gcgggtgcgc cggccgcgac ccgaaccgcg agcgccaccg ccgagcgcgt
     2041 tacatccgac cagtgcccga cgtgcggtcg cccgatcagc gcttggacgt catcgagccg
     2101 ctcgtcttgg ccggagtcga gcacgcgctg caccactcgt aaagcggtcg cgcgttcgag
     2161 ctcctgctcg tccaggagcc caacgtggcc cgcaaacacg atgcctccgg ccgcgtcctc
     2221 gacgtagagc accgcgaaag gaatgtcctc tggattcgac gccagcgccg acatcaacga
     2281 ggccgcacct cccgtctgcg cagccacggc gcgcagtgtc gccgcgcggc gctccgcgag
     2341 tacgaacccg gtcgtctcga tcaccggctg gaagatgccc gcgatgcgtt cggcgtcgtc
     2401 gcggagcgga ctgaacgtga acgtaaagta cgcctcctcg gtgaagccgt ggcgcgtcag
     2461 cgtgaacagc gtattctcca cgtgcttgac ttccccgcgt tcgagcacgt cgaccatcca
     2521 ggggtaaatc accggatatg tatcgggcca gcactcgcga taaggagccc cgaagtactg
     2581 agggtggcga gggcccatga tcaccgcgta gccgtcgttg tagatctggc tctgctcggg
     2641 gccccagaag atgatcgcag gggtcggcat ctccaaaatc atggaaacgt aacttttgag
     2701 ggatcgcggc caactcgcga tgggcccaag ggcagtcttc gcccagtcgt gtctctgaac
     2761 gagctcgcgg caattttcgc ggttcaccga gaacgactat agcccgccgc cgatcacgac
     2821 acccttgcgt tcgcggaagc gctcgctcgc tcgctccggc tgtgattgtc taatccgagc
     2881 gaatgacttc aatcgtgtgt cgttcgacgt gcatgctgca tggccggggg gagggcataa
     2941 atggcctcta accagagggc tcgcgccgtg attcgatgtg ggcgcttcac tgacgacggg
     3001 tcgcgcgact cgagcatgaa gctgaccggt gcttcggttg cggaggaatt ttgcaatgaa
     3061 ctttgccaag atcgccacac tcccgctttt ggcgacgcag ctcgcgtgga gcctttcggc
     3121 ctgta
//
LOCUS       DASTKS010000004         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1783968, whole genome shotgun sequence.
ACCESSION   DASTKS010000004 DASTKS010000000
VERSION     DASTKS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783968"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..922)
                     /gene="mazG"
                     /locus_tag="VFQ35_00070"
     CDS             complement(77..922)
                     /gene="mazG"
                     /locus_tag="VFQ35_00070"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9929044.1"
                     /translation="MGRPFQIPEELPLHEQRGQTFPSLVEIMQRLLAADGCPWDREQT
                     FESLRKYVLEEACEVIDAVDSGSREHLREELGDLALQVAFLGELARQEGSFGPDDVVR
                     AIVEKLVRRHPHVFADADVQDSDEVVRNWERIKAQEKSERGVLDGVPRSLPALYRAQR
                     MSDKVSRVGFDWPDGRGSREKVSEELRELDEAIAGGDKGRIEAELGDMLFALVNLARH
                     AGVDAETALRGSCDRFAARFAHVEGRVKERHGGWPRAGDGSATRGVPLEVMDGYWDEA
                     KREGK"
     gene            1012..1797
                     /locus_tag="VFQ35_00075"
     CDS             1012..1797
                     /locus_tag="VFQ35_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9929045.1"
                     /translation="MSDPVLLKSRQDATLELRLNRPQSKNSLDEALVDELGQSLEAAS
                     ADPALRVIVLSGAGGAFCSGVDLRSAAAELDDRARLEGRLAGFHRVIRAIAGAPQPVI
                     ASVDGPAVGFGADLAYACDLRVASTRAYFEEKFVAIGLMPDGGGTFHLPRLIGLGRAL
                     EHQLLGTRFDAERALALGLVSRVVAPEELGAETNRVSTQLALAAPLAVRAIKRALRES
                     ADGTLDDALERERRGQLGLLASADAREGVSAFLGRRAPAFSGS"
     gene            complement(1803..>2555)
                     /locus_tag="VFQ35_00080"
     CDS             complement(1803..>2555)
                     /locus_tag="VFQ35_00080"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9929046.1"
                     /translation="MILAAECSGVLKPGATIIEATSGNTGISLAMIAAVRRYRCMLVM
                     PDDMSLERRHILRAYGADIVLTPGGDGMSGAVSRAQALLAETPGAFMPSQFTNEANPE
                     SHEHGTAREILEQTDGKIAALVAGVGTGGTITGIGRVLRRELGQKVRIVAVEPANSAV
                     LSGKAPGMHGIQGLGAGFVPQILDRSVIDEVVAVTDVAAERMARRLAREEGLLVGPSS
                     GANVHAACEVAARVGGGNVVTILCDSGERYLF"
BASE COUNT          383 a          910 c          827 g          435 t
ORIGIN      
        1 tgctcccact cttcgggcga acgcgcgatg aggccgaggt tcgtggcgac cgccgccgga
       61 agttcagttg tgtcgctcat tttccttcgc gcttggcttc gtcccagtaa ccatccatga
      121 cctcgagcgg cactccgcgc gtggcggagc cgtcgccggc gcgcggccag ccgccatggc
      181 gctctttgac gcgcccttcg acgtgcgcga agcgcgccgc gaagcgatcg cagctgccgc
      241 gcaacgcggt ctcggcgtcg acgccggcgt gacgcgccag gttcaccagg gcaaagagca
      301 tgtcgccgag ctcggcttcg atgcgtcctt tgtcgccccc ggcgatggcc tcgtcgagct
      361 cacgcagctc ctcgctcacc ttctcgcgag agccgcgccc gtcaggccaa tcgaagccga
      421 cacggctcac cttgtcgctc atgcgctgcg cacggtagag cgccggcaac gaacgcggca
      481 cgccgtcgag cacaccgcgt tcgctctttt cttgagcctt gatgcgctcc cagttccgca
      541 ccacctcgtc gctgtcttgc acgtcggcgt cggcgaaaac gtgcgggtgg cgccgtacga
      601 gcttctccac gatggcgcgc acgacgtcgt cgggaccgaa gctcccctcc tgccgcgcga
      661 gctctccgag gaacgccacc tgcaacgcca agtcgccgag ctcttcacgc aagtgctcac
      721 ggctgccgga gtcgacggca tcgatgacct cgcaagcttc ctcgaggacg tacttgcgca
      781 gactctcgaa cgtctgctcc cgatcccagg ggcaaccgtc ggcggcgagc agccgctgca
      841 tgatttcaac gagcgacggg aacgtctgac cgcgctgctc gtggagaggg agctcttcgg
      901 ggatctgaaa ggggcgaccc atggtgactc gcaaaaaccc ggcttttgtg ggtccgggag
      961 gcgacttcct agcaccttct gcggccgttc gcccgggtgt atcgtttcta gatgagcgac
     1021 cccgtgctgc tgaaatcgcg ccaagacgcc acgctcgagc tccgcttgaa tcgcccccag
     1081 agcaagaact cgctcgatga ggcgctggtc gacgagctcg ggcagtctct cgaagccgcg
     1141 agcgcggacc ccgcgcttcg agtcatcgtg ctgagcggcg cgggcggcgc tttctgctct
     1201 ggcgtggatc tgcgctcggc ggcggccgag ctcgatgacc gagcgcgcct cgagggccgt
     1261 ctcgctggct ttcatcgcgt cattcgggcg atcgccggcg cgccgcaacc cgtcatcgcg
     1321 tcggtggatg ggcccgcggt gggattcggc gcagacctcg cctacgcttg cgatctccgc
     1381 gtcgcatcga cccgcgccta cttcgaagag aagttcgtcg cgatcggact gatgccggac
     1441 ggaggcggga ccttccactt gccccgcctg atcgggctcg gccgcgctct cgaacatcag
     1501 ctgctgggca cccgtttcga cgccgagcgg gcgctcgctc tcgggctcgt gagccgagtc
     1561 gtcgcccccg aagagctcgg ggccgagacg aaccgcgtct cgacccagct cgcccttgcc
     1621 gcgccgctcg ccgttcgcgc gatcaagcgc gcgttgcgcg aaagcgccga tgggaccctc
     1681 gacgatgcgc tcgaacgcga gcgccgaggt caactcgggc tgctcgcgag cgcggacgca
     1741 cgcgaaggcg tgagcgcctt cctcggacgg cgcgcgccgg ctttctccgg cagctgaccc
     1801 tctcagaaaa ggtagcgctc gcccgagtcg cacaggatcg tgacgacgtt gccgccgccg
     1861 acgcgagctg cgacttcgca cgcggcgtgc acgttggcgc ccgaagacgg ccccaccaac
     1921 aacccctctt cccgagccaa acgccgcgcc atgcgttcgg cggcgacgtc ggtgacggcc
     1981 acgacctcgt cgatgaccga gcgatccaag atctgcggga cgaagcccgc gccgaggccc
     2041 tgaatgccgt gcatgccggg cgccttgccg ctcagcacgg cgctgttcgc gggttcgacc
     2101 gcgacgatgc gaaccttttg cccgagctcg cggcggagca cgcgaccgat gccggtgatg
     2161 gtccccccgg tcccgacccc ggcgaccaaa gccgcgattt ttccgtcggt ttgttcgagg
     2221 atttcgcggg cggtaccgtg ctcgtggctc tcggggttgg cttcgttcgt gaattgactc
     2281 ggcatgaacg cgcccggcgt ctccgcgagc agcgcttgag cgcgactcac ggcgccgctc
     2341 attccatcgc caccgggcgt gagcacgatg tcggcgccgt acgcgcgcaa aatgtgtcga
     2401 cgctcgaggc tcatatcgtc cggcatcacc aacatgcagc ggtagcgccg caccgcagcg
     2461 atcatggcga ggctgatccc agtgttgccg ctggtcgctt cgatgatggt cgcgcccggc
     2521 ttcagcacac cgctgcactc ggcagcgaga atcat
//
LOCUS       DASTKS010000005         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_669264, whole genome shotgun sequence.
ACCESSION   DASTKS010000005 DASTKS010000000
VERSION     DASTKS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669264"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFQ35_00085"
     CDS             <1..435
                     /locus_tag="VFQ35_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929047.1"
                     /translation="SAAEPGVEPRAAAGRDLGAPLARSFAPKSSAGASHQAAPTELAG
                     SERRLTQKATSATFAGELAQIDAARERLRAGDASLALELVRTYQSSFPDGQLRPEALA
                     IAVEAELRSGRSAEARATAARFFSAYPNHPLAARIRALLGED"
     gene            623..1984
                     /locus_tag="VFQ35_00090"
     CDS             623..1984
                     /locus_tag="VFQ35_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HET9929048.1"
                     /translation="MRNELRSLSLACLLVTVACSGADSGSESRASGGVVGKPPEGSGG
                     GGAGGAGGSPTNASGGRVAGNGGALGSGGEVANGGAMTSVGGRTGAEAGRAGSGGASS
                     SSGGRAALGGAPATSGGTSATPGGASTAGGGAGANRGGAAGASNGGSAGSGGTSTSSV
                     CPPGITQTITVAPSGGQFTSLQAAIDSIPNGSTAHVRIDIRAGKYREKVTIAGRSNLC
                     LVGENAMTTVLTYDDSNGKVGSTSGSASVFVSANDFSAANLTFENSFGSGSQAVALRT
                     TGQRQQFSSCRFVGYQDTLYTHQGLQYFRDCYVQGNTDYVFGGATAILQNCEVRNVEG
                     GSAVAAPNTDSGVRYGIVFLGGRFTAVSSLKANSVALGRPWGADGAAAYLQVNLGAHI
                     ASAGFVAMSGNQPEKARFREYESSGPGANASAHPNYEMTATEAAAFTVQTVLGGWTPS
                     YSQ"
     gene            complement(2013..2522)
                     /locus_tag="VFQ35_00095"
     CDS             complement(2013..2522)
                     /locus_tag="VFQ35_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9929049.1"
                     /translation="MRKATGSVHVFTFKEGVLSAAAHDLRLSAQKFDVALDGDRVRAE
                     FDLKALVVEGPMENGVLNAAHYDASKRAEVEKAMHGSVLHTEKHPKAVFAGTATESPN
                     GFEVNGELELVGRRAPLSFSVRKDGTMFRSAFEFNPSKWGIAQYKALLGAIRLKDVVR
                     IEFSLTEES"
BASE COUNT          459 a          858 c          897 g          439 t
ORIGIN      
        1 tcggcagccg agccaggcgt agaaccgcga gcggcggcgg ggcgcgacct gggcgctcct
       61 ctcgcacgct cgttcgcacc gaagtcgtcg gcaggcgcga gtcaccaagc cgcgccgacc
      121 gagctcgcag gctccgagcg acggctcacg cagaaggcca cgagcgcgac gttcgcaggc
      181 gagctcgcgc aaatcgacgc cgcgcgagag cgtctccgcg cgggcgacgc gagcttggcg
      241 ctcgaactcg tgcggactta tcagtcgagc tttcccgatg ggcagctacg tccagaggct
      301 ttggcgatcg ccgtcgaggc cgagcttcgc agcgggcgct cggccgaagc gcgagcaact
      361 gcagcgcgtt ttttctccgc gtacccgaac catccgctcg cggcgcgcat tcgcgctcta
      421 ctcggcgaag actgatgcct caccatcgtc atcgacgatg acggtggctc gatcgtgtag
      481 ggcgggtcct agtcggtggc aaaacgcatg aagttgagcc aatggctcga gcttgggcct
      541 ctccccgtcc gagaggaact tgagccgtag ggcatgcctc gcgacactgt tcttgaattg
      601 tcgctactct cgaggcattc ccatgcgaaa tgagctccgc tcgctgtcgc tcgcgtgcct
      661 tctcgtcacc gtcgcatgct cgggcgcaga ctccggctcc gagtcacgag cgagcggcgg
      721 cgtggttggg aagcctcccg agggcagtgg cgggggcggg gctggcggcg ccggaggttc
      781 accaacgaac gcgagcggtg ggcgcgtcgc aggcaacggc ggtgcgttgg ggtccggcgg
      841 cgaggtcgcc aacggcggcg ccatgacgag cgtgggtggt cgcaccggcg cggaggcagg
      901 tcgcgctggc agcggcggcg ccagttcgtc atcgggaggg cgcgcggcgc tcggcggtgc
      961 gcccgcgacg agcggaggca cttcagcgac cccgggcgga gcttcgaccg ctggcggtgg
     1021 cgcgggggcg aaccggggcg gcgctgcggg cgcgagcaac ggaggctccg caggcagcgg
     1081 cggcacgagc acgtcgtctg tttgcccgcc gggcatcacc cagacgatca ccgtcgcgcc
     1141 gagtggaggg cagttcacgt cgctccaagc cgctatcgat tcgataccga acgggagcac
     1201 ggcgcacgtc cgcatcgata tccgcgcggg gaagtatcgc gaaaaggtca cgatcgccgg
     1261 gcgctcgaac ttgtgcctgg tcggcgaaaa cgcgatgacg acggtgctca cctacgacga
     1321 tagcaatggc aaagtcggca gcacgagcgg cagcgccagc gtcttcgtga gcgcgaacga
     1381 cttttccgcg gcgaacctca ccttcgaaaa cagcttcggc agtggctcgc aagcggtcgc
     1441 gctgcgaacg acggggcagc gccagcagtt ttcgagctgt cgcttcgtgg gctaccagga
     1501 cacgctctac acccaccagg ggctacagta ctttcgcgac tgctacgtgc agggcaacac
     1561 cgactacgtc tttggtggcg ctacggcgat tctccagaac tgcgaagtcc gcaacgtcga
     1621 gggcggctcg gcggttgcgg ccccgaacac cgactccggc gttcgctacg gcatcgtatt
     1681 cctgggaggt aggttcaccg cggtctcgtc gctcaaggcg aactcggttg ccttggggcg
     1741 gccgtgggga gccgacggcg cggccgccta cttgcaggtg aacctcggcg cgcacatcgc
     1801 gtccgcgggc ttcgtggcga tgagcggaaa tcagccagag aaggcgcgtt ttcgcgaata
     1861 cgagagtagc ggtcccggcg cgaacgcgag cgcgcaccca aactacgaga tgaccgcgac
     1921 cgaagcggcc gctttcaccg tgcagaccgt gctcggcggg tggactccga gttacagcca
     1981 gtagcgacca atgcgggcac gtagaacgtg gatcaactct cctcggtcag gctgaactcg
     2041 attcgcacga cgtccttcag ccgaatggcg ccgagcaggg ccttgtattg agcaatgccc
     2101 cacttgctcg gattgaattc gaaggcggag cgaaacatcg tgccgtcctt gcgcacactg
     2161 aaagaaagcg gcgcgcgccg gcccaccaac tcgagctcgc cgttcacttc gaatccgttg
     2221 ggactctcgg tggcggttcc agcaaacacg gctttcgggt gtttttccgt gtgcaacacc
     2281 gacccatgca tcgccttctc gacctcggcg cgcttgctcg cgtcgtaatg ggcggcgttg
     2341 aggacgccgt tctccatcgg gccttccacc acgagcgcct tcaaatcgaa ctcggcccgc
     2401 acccggtcac cgtcgagggc tacgtcgaac ttttgcgccg agagtcgcaa gtcgtgagcc
     2461 gccgccgaca agacgccctc tttgaaggtg aacacgtgaa ccgagccggt cgcttttcgc
     2521 atgaccctag gggtacaccc ggtttcgacg cgcttcaaag cgctacgcac atccttcgat
     2581 ccaggaacag tgtcagattg cagactgcac gcgatgcttg gttcgcgcgg cgcttggcct
     2641 cgaacaattg ctg
//
LOCUS       DASTKS010000006        10179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_669319, whole genome shotgun sequence.
ACCESSION   DASTKS010000006 DASTKS010000000
VERSION     DASTKS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669319"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFQ35_00100"
     CDS             <1..559
                     /locus_tag="VFQ35_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9929050.1"
                     /translation="WLQGATLGLALAAAAAASDGSRPHKTERYDVKAQGFNAGAARTM
                     VFAPDGVVRSVVTDFGKYETFITKFKSAKIVGKVGDKTDVYLQVPILNGAVKIWAVVR
                     FDPPKANGGDQIITGKMVKGNVKRLDATWRIHKVNDESTELRLELGIVPDLPVPESLV
                     LPEVKYAADKAVSGTRDEAERRAAR"
     gene            639..3449
                     /locus_tag="VFQ35_00105"
     CDS             639..3449
                     /locus_tag="VFQ35_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9929051.1"
                     /translation="MHPSDTWLLVPTERHVEALAAAGTQATTLRKLVAELAAAEAPEL
                     LPTTPEATRLLIAELVGCRIGHAKALDDAIGRLRRAGTRAESLRATRLRRGERFADLL
                     GATNDRLTSQKLRDDREAAWLAARYLNRSTELATGATRAVVRGLSRWDPSTLAFLEAL
                     HAALRRRGGEGVVIELPEHGVPALSGSVQALATELEARWAHENDHPSLTFESTEALAK
                     GRVELVETMDPASEARAAARAVLEALARGVALDRIAIVPVDLSEAFLEPLRFELLRAK
                     IPFVEPRGRPVIAAPRAHAALELLRLARGPLTRDGLVDVLRVPGLSVERWFGSVAALE
                     LAHELEKLPIRVERVAGDLVAELEQRVQRLAAEQRMDAAQAATMRDGLSLFLAELAAL
                     GGDAPRGAHARRFRAFFDELGLLATSPREVERALSRERHGAPELLLGLGHDAVASAAV
                     RSASERAVAAAVALGLGEREVRLDTWLEELELALEGVAPSRGAARAAAVRIARTDDVA
                     FMDLGLVVLCRASDGSLDRSTSLDATLGPELENKLPKRERPPGANVEQHFSSLGVAAA
                     LARAESVVVTWSRRDESASTLLPSRLARSLIERSGVRREPGSPLSPRARRAAARKAPS
                     PNATERTRVERARAAFYASPDCAPDALNGEAGDLRAFFGAVPERPVAITAIERGLRCS
                     FLAFASQVLRASRLDPVGDAIGARERGSLLHEALAAALQESSGVRENGTIEEFVEQGV
                     QAAARLLEQKGKSPLRRVGLRSTLADVRAVLRLLAAKDDGMGFRLAEHGFGTGEGWAP
                     LSVGSFMTSGRIDRVDASPDGRRVRVLDYKTRTPARADESLLLQPWLYAEKAARELGA
                     SEVEFCFVGVDGRTPSARVVYSGPLDGEPLREAEHRALTVLTALSDGHVAARPASGAF
                     CPRCDARDICRRPLSSPEGTDE"
     gene            3446..7033
                     /locus_tag="VFQ35_00110"
     CDS             3446..7033
                     /locus_tag="VFQ35_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9929052.1"
                     /translation="MSAPHLPGLERNCIVAASAGTGKTHLITNIYIAHVLGLRSDGEE
                     IAPERIVATTFSRAAAREIRERLEQRLHAIAFPAEAVATGLDPTLASVAELRGLSRST
                     LEKRAATALELLPRTLIDTLHGLATRVLRGHALEFGIAPGFSILDEQEALEDATSTIE
                     DVLSQALAEGPEREAAVTAFIDAGLGLEATITSLMATLSLLDEEGLDAADLLLPAHDA
                     DARGLYESLEHAAAAVLAAGASHPLASAARAVQLGLRALPETEPLSEALGLFFKDKRP
                     QLERMENGRAVLDALDALLRPKDSKAERIRKSVGFLARAPQLLSQERELRTLLARVQR
                     EVLARRRARNAFGFGDLLRLARDALRDAPAIAVEASGALDLLLVDEFQDTSRVQRDLV
                     LLLHERAESARRRAPGALPKPTDLRSRGLVVVGDRKQSIYAFRGADVSIYARLAAELA
                     GAAAVSALDIHGIEPNPEPVAEFVSLKQNYRSAPPILDFVNRVARVDFSQTPKRSFEI
                     RYMEAEELSPPPSRAGVAGRVTFITDDGALPEAPDALLAGAKGPLRTALIAAGFCAKA
                     HDEGQEYRDLALLARRRSSLPLLELALDRLKIPFVVAGRALYATQEVRDLAALLRLSL
                     DPYDRHALATVARGALGGLSDRALAELSVPQRGLLVSSQWARVELGDAGDSARCRALA
                     ERLSELYEIAPRLSPRDVLSLAIERFELESVLGALPRGAVRFGNVGRLLEIAARRGGS
                     LPQFSRWLERQIALEVDESEAATFSEEDDAVRLLTVHGSKGLSFPVTIVVDVNATEPA
                     RSQPFGLLRSDHAAPTLTIRYRADDGLVQSPALARINDDAQARALSERQRLSYVALTR
                     AERELVLVLPAEAPRAGSLGRSVAELLLLDPELPVQKLPALELLQRGPTPFEPDDASE
                     AVPLLPARAPWQGAAIGVTALSDYALCARRFQLLHVAGISEFGSAQEQRGDEASDTAR
                     AIGSAAHQVLESYPVASFGETASEPALLELLAATGLDREAKSTRETAAGIGRFLASDY
                     AREVREHGKVVHREVEMTLVIDESRRARPVRQLELFHTPNAQHRSVLRATLDLVVERD
                     DGSIDVIDYKRSRGGDPTRYALQLHAYRLAVEQRFGEKKIRTGLINLLAPSAEPVWVN
                     EPPPRELAELMALLAESRYRAHYPPIQRARCVSARCGFVTACHPRTRAH"
     gene            7285..>10179
                     /locus_tag="VFQ35_00115"
     CDS             7285..>10179
                     /locus_tag="VFQ35_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HET9929053.1"
                     /translation="MPLPGRRGLYDPQFEHDACGVGFVANIKGEASHDIVKKGVDILL
                     NLVHRGACGCDPLTGDGAGILLQVSHAFFRRECDKAGIDLPEAGRYGVGNVFLPRDKT
                     QRRRCQEILEDKISGTGQKLLGWRNVHVNEDALGPLARASVPVIRQVFVGSTTEDEAS
                     FERKLFVIRKWAERTVRESNIASRGMFYLPSLSSRTIVYKGLLLAEQLTGFFRELEDP
                     DMVSALAMVHQRFSTNTFPTWELAQPFRVLAHNGEINTVRGNSAWMSAREQLFDATQA
                     FGHDIRHILPTIAPGGSDSAQLDNVAELLLHGGRSLPHVMMMLVPEAWQNDPMMPEHK
                     RDFYEYHSCLVEPWDGPAALAFSDGKRIGGILDRNGLRPARWTITKDGLVVLASETGV
                     LDFAPENIERKGRLEPGRMFLVDTEKGRIVEDEEIKDEICKRRPYGKWIRENKISLSE
                     IESSPPSATYREKSSLFERQRVFGYTEEDVRLLMGPMGEKGEEAVGSMGTDTPLAVLS
                     DQPQLLFNYFKQHFAQVTNPAIDPIREELVMSLKAYIGGEGNVLNELPDQAQMLELEG
                     PILTNADLAKLRETHLQQVRVPPTLTMLYPVAEGGAGLKEALDELCRQASLAVLNGYS
                     LLILSDRGADEHMAPVPSLLATGAVHHHLMRNGTRMRIGIIVETGEAREVHHFCLLTG
                     YGAGAINPYLAFETLDQGIREGTLPGVTDPEVAKKKYIKAINKGLLKVMSKMGISTLQ
                     SYHGAQIFEAIGLSPEVVDRYFTGTASRISGVDLDVIAEECRRRHEKAFPKVSRSLPM
                     LDLGGQYHYRAQGERHLWNPTTVARLQHAVRAEDAKSYKEYAEAINNANDGFITLRSM
                     WELSSTRAPVPLEEVESAAELVKRFATGAMSFGSISAEAHENLAIAMNRIGGRSNTGE
                     GGEREERFFPDANGDLRRSSIKQVASGRFGVTTYYLVNADELQIKVAQGAK"
BASE COUNT         1686 a         3536 c         3390 g         1567 t
ORIGIN      
        1 ctggcttcag ggcgcgaccc tgggattggc gctcgcggcc gctgctgctg caagtgacgg
       61 cagccgaccg cacaagaccg agcgctacga cgtcaaagcg cagggcttca acgccggcgc
      121 ggcacgaacg atggtgttcg cccccgacgg ggtggtgcgc tcggtcgtga ccgacttcgg
      181 gaagtacgag accttcatca cgaagttcaa gtcggcaaag atcgtcggca aagtcggcga
      241 caagaccgac gtctacttgc aggtgccgat cttgaacggc gccgtaaaga tctgggcggt
      301 cgtacgtttc gacccaccta aagcgaacgg tggggaccaa atcatcaccg gcaagatggt
      361 gaagggcaac gtcaagcgtc tggacgcgac ctggcgaatt cacaaggtta acgacgagag
      421 caccgagctt cgcttggagc tcggcatcgt tcccgatctg ccagtgcccg agtcgttggt
      481 attgcccgag gtcaagtacg cagcggacaa agccgtgtcg ggcacccgag acgaagcgga
      541 gcgccgcgcc gcgcggtgag cgacggataa agaaagaccg agctttggcg cactcgcgtt
      601 gacgagtgcc ggatgcgtag gtaccgaacg ccctgcgcat gcatccgagt gacacctggc
      661 tgctcgtgcc gaccgaacgg cacgtagagg cacttgcagc cgcgggaacg caagcgacca
      721 cgctgcgtaa gctggtcgcg gagcttgccg cggcggaggc cccggagctg ctcccgacga
      781 cgcccgaggc gacgcggctg ctcatcgccg agctcgtcgg ttgccgcatc gggcacgcca
      841 aggcgctcga cgacgcgatc gggcgcttac gccgggcggg aacgcgcgcc gaatcgctga
      901 gagcaacgcg gctgcgtcgg ggcgagcgct tcgccgacct tttgggcgcc accaacgacc
      961 ggctcacgtc gcagaagctc cgcgatgatc gcgaggccgc gtggctcgcg gcgcggtacc
     1021 tgaaccgctc gaccgaactt gcgacggggg caacgcgagc cgtcgtgcgg ggactgtcgc
     1081 gctgggatcc ttccacgctc gcgtttttgg aagcgcttca cgctgcgctg cggcgccgcg
     1141 gtggcgaagg cgtcgtcatc gagctgcccg agcacggcgt gccggcgcta tccggctcgg
     1201 tgcaagccct cgcgacggag ctcgaagcgc gctgggctca cgagaacgat cacccgagcc
     1261 tcaccttcga gtcgaccgag gcgctcgcca aaggccgggt cgagctcgtc gaaaccatgg
     1321 atccggcgag cgaagcgcgc gcggcggctc gcgccgttct cgaagcgctg gcgcggggcg
     1381 tcgcgctcga tcgcatcgcg atcgttccgg tggatctctc cgaggctttc ctcgagccgc
     1441 tccgattcga gctcctgcgc gccaagattc cgttcgtcga gccgcgcggg cgtcccgtca
     1501 ttgccgctcc gcgcgcgcac gctgcgctgg aactgcttcg gctcgcccgg ggaccgctca
     1561 cgcgcgacgg gttggtcgat gtccttcgtg ttccggggtt gtccgtggag cgctggttcg
     1621 gttcggtggc agcgctcgag ctcgcccatg agctcgaaaa gcttccgatc cgggtagagc
     1681 gagtggcagg cgacttggtg gcggagctcg agcaaagagt tcagcgcctc gcggccgaac
     1741 agcggatgga cgcggcgcaa gcggcgacca tgcgcgacgg cctctcgctc ttcctcgcgg
     1801 agctcgccgc gctcggcggt gacgctccgc ggggagcgca cgcccggcgc tttcgcgcgt
     1861 tcttcgacga actcggattg ctcgccactt cgccgcgcga ggtcgagcgg gcgctgtcgc
     1921 gcgagcggca cggcgcaccc gagctcttgt tggggctcgg gcacgacgcc gttgcctccg
     1981 ccgcggtgcg gagcgcgagc gagcgcgcgg tcgcggccgc cgtggcgctc gggctcggtg
     2041 agcgcgaagt tcggctagac acatggctcg aagagctgga actcgccctc gaaggcgtgg
     2101 cgccttctcg cggcgcggct cgcgcggcag cggttcgcat cgcgcgcacg gacgacgtcg
     2161 cgttcatgga cctcgggctc gtcgtgttgt gccgcgcgag cgacggcagc ctcgaccgga
     2221 gcaccagcct cgacgcgacg ctcggtcccg agctcgaaaa caagctaccg aagcgcgaac
     2281 gtccgccggg cgcgaacgtc gaacagcact tctcgtccct cggcgtcgcc gcggcgctcg
     2341 cgcgtgccga gtcggtcgtc gtcacgtggt cgcgccgcga cgagagcgct tccacgctgc
     2401 tgccgagccg gctcgcgcgg agcctcatcg aacgctcggg cgtgcgacgc gagcccgggt
     2461 ctccattgtc tcctcgagcg cgccgcgccg ccgcgcgcaa agctccgagc ccaaacgcga
     2521 ccgagcgcac gcgtgtcgag cgggcgcgtg cggccttcta cgcgagcccc gactgcgccc
     2581 cggacgcttt gaacggcgaa gccggcgatc tccgcgcctt tttcggggca gttccggagc
     2641 gaccggtcgc aatcacggcc atcgagcgag gcctgcgctg ttcgttcttg gcattcgcga
     2701 gccaagtctt gcgagcgagc cgcctcgacc cggtcggaga tgcgatcggc gcgcgagagc
     2761 gcggctccct cctccacgag gcgctcgcag ctgcgctcca agagagctcc ggcgttcgcg
     2821 aaaacggcac catcgaagag ttcgtcgagc agggcgtgca ggcagccgcg cgccttttgg
     2881 agcaaaaggg caagagcccg ctgcgccggg ttgggctccg gagcacgctc gcggacgtgc
     2941 gcgcggtgct tcgcttgctc gccgcgaagg acgacggcat gggctttcgc ttggccgagc
     3001 acggcttcgg tactggcgaa ggctgggctc ccctcagcgt cggctcgttc atgacctccg
     3061 ggcgcatcga tcgcgtggac gcgagccccg atggtcgccg cgttcgagtg ctggattaca
     3121 agacgcgcac cccggcgcgc gccgacgagt cgttgctgtt acagccctgg ctgtacgccg
     3181 aaaaggcagc gcgcgagctc ggcgcgagcg aagtcgagtt ctgcttcgtg ggcgtcgacg
     3241 gtcgcacgcc gagtgcgcgc gtcgtctact cggggccgct cgacggggag ccgttgcggg
     3301 aagccgaaca ccgcgccctc accgtgctca cggcgttgag cgatggccac gtcgccgcgc
     3361 gcccggcgtc gggagccttt tgcccgcgct gcgacgcgcg cgacatctgt cgtcgtccgc
     3421 tctcgtcgcc ggaaggcacc gacgaatgag cgcgccccat ttacccgggc tcgaacgaaa
     3481 ctgcatcgtc gccgccagcg ccggcaccgg taagacccac ctcatcacga acatctacat
     3541 cgcccacgtg ctcggcctgc gcagcgatgg cgaagaaata gcccccgagc gcatcgtcgc
     3601 gacgacgttc tcgcgagcgg cggcgcggga aatccgcgag cgcctcgagc aacgcctgca
     3661 cgccatcgcc tttcccgccg aggcggtggc gactgggctc gatccgacgc tcgcgagcgt
     3721 cgccgagctc cgcgggctca gccgttcgac gctcgaaaaa cgcgctgcga cggcgctcga
     3781 gctcctgcca cgaaccctga tcgacaccct gcacggcctc gcgacccggg tgttgcgggg
     3841 acacgccctc gagttcggga tcgcgccggg cttcagcatc ctcgacgagc aagaggcgct
     3901 cgaggatgcc acctcaacca tcgaagacgt gctgtcgcaa gcgctcgccg aaggcccgga
     3961 gcgagaagcg gcggtgacgg cgttcatcga cgccggcctc gggctcgaag ccacgattac
     4021 gagcctgatg gcgacgttgt cgttgctcga cgaagagggg ctcgacgccg ctgatttgct
     4081 attgccggcc cacgacgcgg atgcgcgagg cttgtacgag agtctcgagc atgcggccgc
     4141 ggccgtgctc gccgcgggag caagccaccc gctcgcttcg gctgctcgcg ccgtgcaact
     4201 cggactgcgc gccctgccgg agaccgaacc gctgagcgag gccctcgggc tctttttcaa
     4261 ggacaagcgc ccgcagctcg agcgcatgga gaacggccgc gcagtgctcg acgcgctcga
     4321 cgcgctgctc cgcccgaaag actcgaaggc ggagcgcatc cgcaagagcg tcggcttcct
     4381 cgcccgtgcc cctcagctct tgtcacaaga gcgtgagctg cggacactcc tcgcgcgcgt
     4441 ccaacgcgag gtgctggcgc gacgccgcgc tcgcaatgcc ttcggcttcg gcgatctgct
     4501 gcggctcgca cgcgacgcgc tgcgggacgc gcccgcgatc gccgtcgaag cgagcggcgc
     4561 gctcgacttg ctcttggtcg acgaatttca ggacaccagc cgagtgcagc gcgacctcgt
     4621 gctcttgctg cacgaacgcg ccgaatcggc gcggcgacga gcgcccggcg cattgcccaa
     4681 accgacggac cttcgctcgc gcgggctcgt ggtcgtcggc gatcgcaagc agtcgattta
     4741 cgctttccgc ggcgcggacg tgtcgattta cgcgcgactc gccgcggagc tcgccggcgc
     4801 ggccgcggtg agcgcgctcg acatccacgg tatcgagcca aatccagagc ccgtcgccga
     4861 gttcgtgagc ctcaagcaaa actatcgctc tgcgccgccc atcctcgact tcgtgaaccg
     4921 cgtcgcgcgc gtcgactttt ctcaaacccc gaagcgctcc ttcgaaatcc gctacatgga
     4981 ggccgaggag ctctcgcccc cgccgtcgcg tgcgggcgtc gccgggcgcg tcaccttcat
     5041 caccgacgac ggagcgctgc ccgaggcgcc cgacgccctc ctcgcgggcg cgaagggccc
     5101 tctgcggacg gcgctcatcg ccgccggttt ctgcgccaaa gcgcacgacg aagggcagga
     5161 gtatcgagat ttggcgctgc tcgcccggcg tcgctcgagc ctaccgctgc tcgaactcgc
     5221 gctcgatcga ttgaagattc cgttcgtcgt cgctgggcgc gcgctctacg ccacgcaaga
     5281 ggttcgcgat ctcgcggccc tgctcaggct gagcctcgat ccgtacgatc gccacgcact
     5341 cgccaccgtc gcacgcggcg cgctgggagg gctttcggac cgcgcgctcg ccgagctctc
     5401 ggtaccgcag cgcgggctct tggtttcgtc ccagtgggcg cgcgtcgaac tcggtgacgc
     5461 cggcgacagc gctcgctgcc gcgcactcgc cgagcgactg agcgagctct acgagatcgc
     5521 tccgcgcctg tcgccgcgcg acgtattgtc gctcgcgatc gagcgcttcg aactcgagtc
     5581 ggtgctcggc gctctgccgc gcggcgcggt ccgtttcggc aacgtcggtc gcttgctcga
     5641 gattgcagcg cgtcgcggcg gaagcttgcc gcagttttct cgctggctcg agcggcaaat
     5701 cgcgctcgaa gtggacgaga gcgaagccgc gaccttctcc gaggaagacg acgccgtgcg
     5761 cctgctcacc gtgcacggca gcaagggatt gtctttcccg gtcaccatcg tcgtcgacgt
     5821 gaacgcgacc gaaccggcgc gctcgcagcc cttcggcctt ttgcgcagcg accacgctgc
     5881 gccgacgctc acgattcggt accgcgccga cgacggcttg gtgcaatcgc cggcgctcgc
     5941 gcgcatcaac gacgacgcgc aggcgcgcgc tctctccgag cgacaacgcc tgtcttacgt
     6001 tgcgctcacc cgcgccgagc gtgagctcgt gctggtgctc cccgccgaag cgccgcgcgc
     6061 gggaagcctc gggcgctcgg tcgcggagct cctgctgttg gatccggagc tgccagtgca
     6121 gaaactccct gcgctcgagc tgttgcaacg cggacccacg ccgttcgagc ccgacgacgc
     6181 gagcgaggcg gtgccgctgc tgccggcgcg cgctccctgg caaggtgccg ccatcggcgt
     6241 caccgcgctc agcgactatg ccctctgcgc gcggcgcttc cagctcttgc acgttgccgg
     6301 catcagcgag ttcggaagcg ctcaagagca gcgcggagac gaagcctcgg acacggcgcg
     6361 cgcgatcgga agtgccgcgc atcaggtgct cgaaagctac cccgtcgcaa gcttcggcga
     6421 gacggcgagc gagcctgcgc tgctcgagct cctcgccgcc accgggctcg accgcgaggc
     6481 aaagagcacg cgagaaactg cggccggcat cgggcgattc ctagcgagtg actacgcgcg
     6541 cgaggtgcgc gaacacggca aggtcgtgca tcgcgaagtc gaaatgaccc tcgtcatcga
     6601 cgagtcgcgg cgagcgcgcc cggtgcggca gctcgagctc ttccacacgc cgaacgcaca
     6661 gcatcgcagc gtgctccgcg cgacgctcga tctcgtcgtc gaacgcgacg acggcagcat
     6721 cgacgtcatc gattacaagc gctcgcgggg tggtgacccg acgcgctacg cgctccagct
     6781 gcatgcctat cgcctcgcgg tggagcagcg cttcggcgag aagaaaatcc gcaccgggct
     6841 catcaacctg ctcgccccct ccgccgaacc cgtctgggtg aacgagcccc cgccccggga
     6901 gctcgccgag ctcatggcgc tgctcgccga aagccggtac cgcgcccatt acccgccaat
     6961 ccagcgggca cgctgcgtct ccgcccgctg cggcttcgtt acggcgtgcc acccacgcac
     7021 ccgggcccac tagctccgtc cggcgaaagc accggaaagg aggaggaaac cctccggtcg
     7081 ccgaacgcca ccgaggctgt cgtccgagag gagccaatga cgcaaagcgt cacagttccg
     7141 cccacattgg tgcctgacag gaacgcgaag agccatatac ttggctggcc gaagtgcccg
     7201 ccgtgtggca agcccggcga cttccggaac cgcgcctgca cgtaggggcg cacctttcaa
     7261 ccctcaaacc tacaccgaga gacaatgccg cttcctggcc gacgaggact ctacgacccg
     7321 caattcgaac acgacgcttg tggcgttgga ttcgtagcca acatcaaggg cgaggcctcg
     7381 cacgatatcg tcaagaaggg cgtcgacatt ctgctgaatc tcgtccatcg cggtgcttgc
     7441 ggctgtgatc cgctcactgg cgacggggct ggcattctgc tgcaggtttc gcacgccttc
     7501 tttcggcgtg agtgcgacaa ggcgggcatc gatttgcccg aggctgggcg ctacggcgtc
     7561 ggcaacgtct ttctaccccg cgacaagacc cagcgccgcc gttgtcagga gatcctcgaa
     7621 gacaagatct ccggcaccgg acagaaactg ctcggctggc gcaacgttca cgtgaacgag
     7681 gacgcgctcg ggccgctggc acgcgcgagc gtgccggtga tccggcaggt gttcgtgggc
     7741 tcgacgacgg aagacgaagc gagcttcgag cggaagctgt tcgtgatccg caagtgggcg
     7801 gagcgcacgg ttcgcgagag caacatcgcg agccgcggca tgttctacct cccgagcctc
     7861 tcgtctcgga ccatcgtcta caagggcctc ttgctcgccg agcagctcac cggcttcttc
     7921 cgcgagctcg aagatccgga catggtgagc gccctcgcga tggtgcatca gcgcttcagc
     7981 accaacacgt tcccgacgtg ggagctcgct cagccgttcc gcgtgctcgc ccacaacggc
     8041 gagatcaaca cggtccgcgg caactcggcg tggatgagcg cgcgcgagca gctgttcgac
     8101 gcgacccagg cgttcggcca cgacattcgc cacattttgc cgaccatcgc tccgggcggc
     8161 agtgattcgg cgcagctcga caacgtcgcc gagctcctgc tccacggcgg gcgttcgctg
     8221 ccgcacgtga tgatgatgct cgtgcccgag gcctggcaga acgacccgat gatgccggag
     8281 cacaagcgcg acttctacga gtaccactcg tgcttggtcg agccgtggga cggccccgcc
     8341 gcgctcgcgt tcagcgacgg caagcgcatc ggcggcatct tggatcgcaa cggcctgcgc
     8401 cccgcgcgtt ggacgattac gaaggacggc ctcgtcgttc tcgcctccga gaccggcgtg
     8461 ctcgacttcg cgcccgagaa catcgagcgc aagggccgcc tcgagccggg tcgcatgttc
     8521 ttggtcgaca ccgagaaggg tcgcatcgtc gaggacgaag agatcaagga cgagatctgc
     8581 aagcgccgcc cgtacggcaa gtggatccgc gagaacaaga tctcgctgag cgagatcgag
     8641 tcgtcgccgc cgtcagccac gtaccgcgag aagtcgagcc tcttcgagcg gcagcgcgtg
     8701 ttcggctaca ccgaagaaga cgtgcgcctc ttgatggggc cgatgggtga aaagggcgaa
     8761 gaagcagtcg gttccatggg caccgacacg cccctcgccg tgctctcgga ccagccgcag
     8821 ctgctcttca actacttcaa gcagcacttc gcgcaggtca cgaaccccgc gatcgacccg
     8881 atccgcgagg agctcgtgat gagcctcaag gcgtacatcg gcggcgaggg caacgtcctc
     8941 aacgagctgc cggatcaggc gcagatgctc gagctcgaag gcccgatcct caccaacgcg
     9001 gacctcgcga agctgcgcga gacgcacctg cagcaggtgc gtgtcccccc gacgctgacc
     9061 atgttgtatc cggtcgcgga gggcggcgcg ggcttgaagg aagcgctcga cgagctctgc
     9121 cgccaagcgt cgctcgcggt gctcaacggc tacagcttgc tcatcctgag cgaccgtggc
     9181 gcggacgagc acatggcccc ggtgccgagc ctgctcgcca ccggcgccgt gcatcaccac
     9241 ttgatgcgca acggcacccg gatgcgcatc ggcatcatcg tcgagaccgg tgaggcccgc
     9301 gaagtgcacc acttctgctt gctcacgggg tacggcgcgg gcgcgatcaa tccctacctc
     9361 gcgttcgaga ccctcgatca gggaattcgc gaaggcacgc tgcccggggt caccgatccc
     9421 gaggtcgcca agaagaagta catcaaggcg atcaacaagg gcttgctcaa ggtgatgagc
     9481 aagatgggca tcagcacgct gcagagctat cacggcgcgc agatcttcga ggccatcggg
     9541 ctttctcccg aggtcgtcga tcgctacttc acgggcaccg cgtcgcgcat ctccggcgtc
     9601 gacctcgacg tcatcgccga agagtgccgc cgccgccacg agaaggcgtt tcccaaggtc
     9661 tcacgcagcc tgcccatgct cgacctcggc ggccagtacc actatcgcgc ccaaggcgag
     9721 cggcacctct ggaacccgac gacggtcgcg cgcctccagc acgcggttcg ggccgaggac
     9781 gcgaagagct acaaggagta cgccgaagcg atcaacaacg cgaacgacgg cttcatcacc
     9841 cttcgcagca tgtgggagct ctcgtcgacg cgagcgccag tgccgctcga ggaagtcgag
     9901 tccgcggccg agctcgtgaa gcgcttcgcc accggcgcga tgagcttcgg ctcaatcagc
     9961 gccgaagccc acgagaacct cgccatcgcg atgaatcgca tcggcgggcg gagcaacacg
    10021 ggtgaaggcg gcgagcgcga agagcgcttc ttcccggatg ccaacggcga cctgcggcgc
    10081 agctcgatca agcaggttgc gtcgggtcgc ttcggggtca cgacgtacta cctggtcaac
    10141 gccgacgagc tccagatcaa ggtcgctcaa ggcgcgaag
//
LOCUS       DASTKS010000007         5184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1338341, whole genome shotgun sequence.
ACCESSION   DASTKS010000007 DASTKS010000000
VERSION     DASTKS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338341"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFQ35_00120"
     CDS             <1..527
                     /locus_tag="VFQ35_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929054.1"
                     /translation="ALGYSIGGVVRDKDGVSALLWFCELAERLRAQGETLFDQLHRIY
                     ARSGVWRSAQASLFNPGATGIERTRAQVERLTRSKPTTLHGLRVTSTLDYREGAERRR
                     PWLGAAALIELSLEDQSRVLVRPSGTEPKLKIYADARTSPTAGESVAAASERAHRSAS
                     ALAQELARWLEAEA"
     gene            complement(539..1846)
                     /locus_tag="VFQ35_00125"
     CDS             complement(539..1846)
                     /locus_tag="VFQ35_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9929055.1"
                     /translation="MNAPASVNKPTVLVESSHDLPLVSVSVATKTGSLFDPAELEGLT
                     RLTSRLMRRTGGGRDAQQIDTQIDALGGSLAAEVSASATSFSGTVIRRSLEPYLDLLG
                     DVLARPGLADEELGRLQRESHAELVEALDDDRGLARRWFRKYMFEGHPYGRPVSGVSS
                     SISRIAKADVAACAKRLVAREGLIIAFSGDLTHDEAEHHAEKLFAALPSGSPPVDATT
                     DPAPRAGRRLIIVDKPERTQTQILIGGTGTHASDPDHTALLVGNTIFGGTFTARMTQE
                     VRSKRGWSYGAYSSLPIDRRRQAFSMWTFPKAEDAAPCVTLELDMLRALREKGVTKKE
                     LSWAKRYLTRSHAFAIDTPAKRVGLALDARLYDLPENYYEQYLARVAAASLDDVNKAL
                     LERLPEKDLLIVVVGTASTIRAPLEAAIPDLSAVEVVKFDTEV"
     gene            complement(1843..3264)
                     /locus_tag="VFQ35_00130"
     CDS             complement(1843..3264)
                     /locus_tag="VFQ35_00130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9929056.1"
                     /translation="MQAPSAASSSRPAKGALPQKTLRRNALLDKVNAAAGEWGRVEHV
                     ETIAFGPSLKLERFRLKNGLMILLCEDHSAPVVAYHTWFRVGSRHEHEGKTGLAHLFE
                     HLMFNEVEGRPAGEFDRLLEEAGSEVNASTWLDWTQYNAATPRDQLPLVIQLEAERMS
                     RLVLREPQVTSEKEVVANERRYRVEDDVEGAVSEVLWSTAFKSHAYKWPTIGWMADIE
                     GFTTEDCEQFYRTFYSPNNATIVVVGDVTEAQLLPRIVKAYGEIAPSELPLEDARPET
                     PLLEERKVEVTKPTTTEKLLIGYHSPALGDWDHPIISLLVEVLFGGRASRLHQRLVRE
                     LELASEVRAFVGPFKDPGLIEIFASCREGRRAEELLAVIDAELARVREEPIPEAEIER
                     GRARFELGLLAGLDTVDGKASTLGFYDTVLSRPAAAFERLEATRQINASELRRVARKY
                     FPEGKRAIVLVRAQASTAAAEAP"
     gene            complement(3314..5119)
                     /gene="lepA"
                     /locus_tag="VFQ35_00135"
     CDS             complement(3314..5119)
                     /gene="lepA"
                     /locus_tag="VFQ35_00135"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET9929057.1"
                     /translation="MPTDPQKIRNFSIIAHVDHGKSTLADRILDVTGAITARERVDQF
                     LDKLELERERGITIKAQSVRLRYKAKDGQEYQLQLIDTPGHVDFSYEVSSSLRACEGA
                     LLVVDATQGVQAQTVANAYLAIENNLEVIPVLNKVDLPSADVDRAGEQIEEVIGLDCS
                     GAVFASGKTGVGVPDVLEAVVARIPAPKGDANAPLKALLFDSWYDSYRGAVIMVRVME
                     GTLRKGERIKFMATGRTYDVTEIGCFAPHPTALTELGPGEVGFVSANIKSTEDTKIGD
                     TVTLVYNQAPEPLPGFREIKPMVFAGIFPTDSADYENLRDALSKLHLNDASFQFEPDT
                     SDALGFGFRCGFLGLLHMEIVQERLEREYDLDLITTAPSVVYIAHTKTGEAIRVDNPS
                     KMPAVGEIEKIEEPILKLTVHVPSDYVGPVVSLCEERRGVQVGIHYATKDHVVVTYEI
                     PLAEVILDFHDRLKSVSRGYASMDYDIAGYRADNLVKLDMLLNGEPLDALSVIVHKDT
                     AYSRGRALAAKLKELVPQQQYEVAIQAAIGSKVISRETVRALRKDVTAKCYGGDISRK
                     RKLLEKQKEGKRRMKAVGSVELPQDAFLAVLKLES"
BASE COUNT          830 a         1679 c         1745 g          930 t
ORIGIN      
        1 aggcgctcgg gtactcgatc ggcggcgtgg tccgggacaa agacggcgtt tcggcgctgc
       61 tctggttctg cgaactcgcc gaacggctac gcgcgcaggg cgaaacgctc ttcgaccagc
      121 tacaccgcat ctacgcgcgg agcggtgttt ggcgcagcgc tcaggcgtcg ctcttcaacc
      181 ccggggcgac cggcatcgaa cggacgcgcg cgcaggtcga gcgcctgacg cgctcgaagc
      241 cgaccacgct gcatggcctc cgggtgacct caacgctcga ctaccgagaa ggcgcagagc
      301 gccgccggcc ctggctcggc gcggccgcgc tcatcgagct ttcactcgaa gatcagtcgc
      361 gcgtgctcgt gcgcccgagc ggcaccgaac ccaagctcaa gatctacgcc gacgcccgta
      421 cgagcccgac cgctggtgaa tcggttgccg ccgccagcga gcgcgctcat cgaagcgcca
      481 gcgcgctcgc gcaagagctc gctcggtggc tcgaggccga ggcgtagcgt cgagcgcctc
      541 agacctccgt atcgaacttc acgacttcga ccgcgctcag gtccggaatg gcggcttcga
      601 ggggcgcgcg aatggtgctc gcggtgccga ccacgacgat gaggagatcc ttctccggca
      661 ggcgctcgag caaggccttg ttcacgtcgt cgaggctcgc cgccgcgacg cgcgccaggt
      721 actgctcgta gtagttctcc ggcagatcgt agagccgtgc gtcgagcgcg agcccgacgc
      781 gcttcgccgg cgtatcgatc gcgaaagcgt gggagcgcgt gaggtaacgc ttcgcccacg
      841 agagctcctt cttggtgacg cccttttcgc gcaacgcgcg cagcatgtcg agctcgagcg
      901 tgacgcaggg agcggcgtct tccgctttcg ggaaagtcca catgctgaag gcttggcggc
      961 gccgatcgat gggcaggctc gagtacgcgc cgtaggacca accgcgcttc gagcggacct
     1021 cctgcgtcat tcgcgccgtg aaagtaccgc caaaaatcgt gttgccgacg agcagggccg
     1081 tgtgatccgg atccgaagcg tgggtcccgg ttccgccaat cagaatctgc gtctgggtgc
     1141 gctcgggttt gtcgacgatg atcagccggc gtccagcgcg cggcgccgga tcggtcgtgg
     1201 cgtcgacggg tgggctgccg ctcgggagcg cggcgaagag cttctcggcg tggtgctccg
     1261 cttcgtcgtg ggtgaggtca ccggagaagg cgatgatgag gccttcgcgc gcgacgagtc
     1321 gcttcgcgca ggcggcgacg tcggcctttg cgatccgcga aatcgagctc gagacgccgc
     1381 tcacgggacg gccgtaaggg tgcccttcga acatgtactt acgaaaccag cgcctcgcga
     1441 gcccacgatc atcgtcgagc gcctcgacca gctcggcgtg gctctcgcgt tgaagccggc
     1501 cgagctcttc gtccgcgagc ccgggccgcg ccaaaacgtc gccgagcaaa tcgaggtaag
     1561 gctcgagcga gcgccggatg accgttcccg agaacgacgt cgcgctcgcg ctgacctcgg
     1621 ccgcgaggga gccgcccaaa gcgtcgatct gggtgtcgat ttgttgcgcg tcgcgcccgc
     1681 cgccggtgcg gcgcatgagc ctcgacgtga gccgcgtgag cccttcgagc tcggccgggt
     1741 cgaagaggga gccggtcttg gtggcgacgc tcaccgacac gaggggcaag tcgtggctgc
     1801 tctcgaccaa gacggtcggc ttgttgacgg atgcaggagc gttcacgggg cctccgcggc
     1861 ggcggtcgag gcctgcgcgc gcacgagcac gatcgcgcgc ttgccttcgg ggaaatactt
     1921 tcgcgcgacg cgacgaagct cgctcgcgtt gatctgacga gtcgcctcga ggcgctcgaa
     1981 cgcagccgcg ggccgcgaga gaacggtgtc gtaaaacccg agggtggagg ccttgccgtc
     2041 gacggtgtcg agcccggcga gcaacccgag ctcgaaccgc gcccgtccac gctcgatctc
     2101 cgcttcgggg atcggctcct cgcgcacgcg agcgagctcc gcgtcgatga cggcgagcag
     2161 ctcttcggcg cgccggcctt cgcggcacga cgcgaagatc tcgatgagcc cagggtcttt
     2221 gaacggtccg acgaaggcgc gcacttcgct cgcgagctcg agctcccgca cgaggcgttg
     2281 atgcagccgg ctggcgcgac cgccgaagag cacctcgacc aagagcgaga tgatcgggtg
     2341 gtcccagtcg ccgagggcgg gggagtggta gccgatgagg agcttctcgg tcgtggttgg
     2401 cttcgtcact tcgaccttgc gctcctcgag cagcggggtc tcgggacgtg cgtcttcgag
     2461 ggggagctcg ctcggagcga tttcgccgta cgccttcacg atgcgcggca atagctgcgc
     2521 ttcggtgacg tcgccgacga ccacgatggt cgcgttgttc ggcgaataga aggtccgata
     2581 gaactgctcg cagtcctcgg tggtgaagcc ttcgatgtcc gccatccagc cgatcgtcgg
     2641 ccacttgtag gcgtggctct tgaacgcggt cgaccacagc acttcgctca ccgccccttc
     2701 gacgtcgtcc tcgacgcggt agcggcgctc gttcgcgacc acctcttttt cgctcgtcac
     2761 ctgcggctcg cgcagcacga gccgagacat gcgctccgct tcgagctgga tcacgagcgg
     2821 gagctggtcg cggggcgtgg ccgcgttgta ctgagtccaa tcgagccagg tcgatgcgtt
     2881 gacctcggac cccgcttctt cgagcaaacg atcgaactca cccgcggggc gtccctcgac
     2941 ctcgttgaac atcaggtgct cgaagaggtg ggcaagcccc gtcttacctt cgtgctcgtg
     3001 gcgcgagccg acgcggaacc acgtgtggta ggcgacgacg ggcgcgctgt ggtcctcgca
     3061 gaggaggatc atcaggccat tcttcaaccg aaaccgctcg agcttcagcg acggcccgaa
     3121 ggcgatcgtc tcgacgtgct cgacccgacc ccactcgccc gcggccgcat tcaccttgtc
     3181 cagcagggcg ttgcgacgca gcgtcttttg ggggagggca cctttggcgg ggcggctaga
     3241 cgaagccgcg gagggcgctt gcatcccgca cccataccac ggaaacgccc ctctcgaggg
     3301 cgcagcgaga atctcaactt tcgagcttca ataccgccaa gaacgcatct tgggggagct
     3361 cgacgctgcc gacggctttc atccgccgct tgccctcttt ctgcttctcg aggagctttc
     3421 gcttgcgaga gatgtctccg ccgtagcact tcgcggtcac gtctttgcgg agcgcgcgca
     3481 cggtctcgcg gctgatgact ttgctgccga tcgcggcctg aatcgcgact tcgtactgtt
     3541 gctgcgggac gagctccttc agcttcgcgg cgagcgcgcg tccccgtgag taggccgtgt
     3601 ctttgtgaac gatgacgctc agcgcgtcga gcggttcgcc gttcaacagc atgtcgagct
     3661 tcaccaggtt gtcggcgcga tagccagcaa tgtcgtagtc catgctggcg tagccgcggg
     3721 acacgctctt caggcgatcg tggaagtcga ggatgacctc ggcgagcggg atctcgtagg
     3781 tgaccaccac gtgatctttg gtcgcgtagt ggatgccgac ttgcaccccg cgccgctcct
     3841 cgcacagcga caccaccggt cccacgtagt cggatggcac gtgaacggtg agcttcagaa
     3901 tcggctcttc gatcttctcg atctctccga cggccggcat cttcgacggg ttgtcgacgc
     3961 ggatcgcttc gcccgtcttc gtgtgcgcga tgtagaccac gctcggcgcg gtcgtgatga
     4021 gatcgaggtc gtactcgcgc tcgagccgct cctgcacgat ttccatgtgc aagaggccca
     4081 agaagccaca gcggaagccg aagccgagcg cgtccgaggt gtccggctcg aactggaacg
     4141 aagcgtcgtt caggtgcagc ttgctgagcg cgtcgcgtaa gttctcatag tcggccgagt
     4201 cggtcgggaa gatgcccgcg aacaccatcg gcttgatctc gcggaagccg gggagcggct
     4261 cgggcgcctg gttgtaaacg agcgtgacgg tgtcaccgat cttcgtatct tcggtgctct
     4321 tgatgttggc cgagacgaag cccacttcgc ccgggccgag ctcggtcaaa gcggtcgggt
     4381 gcggcgcgaa gcaaccgatc tcggttacgt cgtaggtgcg acccgtcgcc atgaacttga
     4441 tgcgctcgcc ttttcggagc gtaccctcca tgacccgcac catgatgacg gcgccgcggt
     4501 aactgtcgta ccagctgtcg aagagcagcg ctttgagcgg cgcgttcgca tcgcccttcg
     4561 gcgcagggat gcgcgcgacc accgcttcga gcacgtcggg gacgccgacc ccggtcttgc
     4621 cgctcgcgaa gacggcgccg ctgcagtcga gcccgatcac ctcttcgatt tgctcgccgg
     4681 cgcggtcgac gtcggcggaa ggcaagtcca ccttgttgag gacggggatg acctcgagat
     4741 tgttctcgat cgcaaggtaa gcgtttgcga ccgtctgcgc ctgaacgcct tgggtcgcgt
     4801 ccacgacgag cagcgcgcct tcacacgcgc gcaggctgct cgaaacttcg tagctgaagt
     4861 cgacgtgtcc gggcgtgtcg atgagctgca gctggtactc ctgcccgtcc ttcgccttgt
     4921 agcgaaggcg taccgactgc gccttgatgg tgatgccgcg ttcccgctcg agctccagtt
     4981 tgtcgaggaa ctgatcgacc cgctcgcgtg cggtgatcgc gcccgtaacg tccaggatgc
     5041 ggtcggcaag cgtgctcttg ccgtgatcga cgtgagcgat aatcgaaaaa ttgcgaatct
     5101 tctgcgggtc ggtaggcatc gggagtggac cgggtgcgct cgagaacggc gcgggtctag
     5161 cacgagctcg agcgccggca cctg
//
LOCUS       DASTKS010000008         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1338635, whole genome shotgun sequence.
ACCESSION   DASTKS010000008 DASTKS010000000
VERSION     DASTKS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338635"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /gene="rlmN"
                     /locus_tag="VFQ35_00140"
     CDS             <1..1044
                     /gene="rlmN"
                     /locus_tag="VFQ35_00140"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9929058.1"
                     /translation="WIQRRGVFEPNEMSDLSLELREKLSSLGLRSPIEVARVQRSQDG
                     TRKLLLELFDGGRVECVLIPMTRLDAETDADAAAAVDDEPDPEEAEPKTKVTLCISTQ
                     YGCAMGCRFCASGRAGLLRGLKAAEIVAQVLVSKRYLEPNEELKNLVFMGMGEPLHHY
                     DETARALRLLMNTEGLNMSPRRITVSTVGLVPGIQKLGRDFDGKIGLAVSLHAPNDHI
                     RSSIMPMNDRFPIAELMQALRAYPLPRRRRITIEYTLIDGVNDSREHADELVWLLRRL
                     PVKINLIPMNPVEGSGYRAPPAARVSEFQRVLADAGYSCFVRTRRGDEVDAACGQLAL
                     SPDVVELRKKPSA"
     gene            1052..2443
                     /locus_tag="VFQ35_00145"
     CDS             1052..2443
                     /locus_tag="VFQ35_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9929059.1"
                     /translation="MFRKILVLNRGEIACRILRTCRRLGVATVAVYSDADAALPHVKQ
                     ADSAVRLGPAPVKDSYLNLDAIVEAIRSSGAEAVHPGYGLLSESPRLARAVADTGAVF
                     IGPKPEILERLGDKISARALARSVGVEPPPGSASPLDPSDAEAVAAEAERVGYPVIVK
                     AAAGGGGIGMLVVREASELARAVKTCTDRARQAFSDDRVYLERYLERPRHIEVQILVD
                     RAGNGVALGERECSVQRRHQKVLEESPSPASFFTGEAGEQRRQALFAKALRVVHAANY
                     EGAGTVEFVCDASGSAYFLEVNARLQVEHPVTEMVTGLDLVEQQLCIACGEELRHDVQ
                     AVARNGHAVEVRIYAEDPSKNFLPQPGRIETFAFPMTFEGVRIESGFEAGAEVTPYYD
                     PLLAKLIAHGATREIALERLDAALEATQIALVGPKGPRATNLAWLRQVLAEPMLRTGD
                     YDTGLADRVGARS"
     gene            2440..>3639
                     /locus_tag="VFQ35_00150"
     CDS             2440..>3639
                     /locus_tag="VFQ35_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET9929060.1"
                     /translation="MSAELIERARAWVAADPDPATRRELLDLIDAGANAEISERFQAE
                     LDFGTAGLRGEVGAGPARMNRAVVMRAVRALAEHLLAQGAEVRSRPVVVGYDARPSSQ
                     SFAEAATGVLLAAGIAVRWFETPVPTPLVCFAARVYGAAAAVVVTASHNPSRDNGLKI
                     YGGDARQLVAPEDVDVARRRDAVGPAAAIPCVDFARAEGRLDSIPEAIVSQYFDEVEA
                     LLPPRSAEPALRIVYTPVHGVGLELARAGLSRRGFRDVHVVAEQAAPDGTFPTAPFPN
                     PELPGVLDRALELAKRVNADVVVANDPDADRLAAAARTRDGAWQVLSGNEIAVLLADC
                     VLERAPKAPRPLLVTSVVSTPLLSDVARTHGAHCERTLTGFKWIWHAARSLEAEGGLA
                     YAMGCEEA"
BASE COUNT          586 a         1196 c         1261 g          596 t
ORIGIN      
        1 tggattcagc gacgcggggt gttcgagccg aacgagatga gcgatctgtc gctcgagctc
       61 cgcgaaaagc tctcgtcgct cgggctccgc tcgccgatcg aggtcgcgcg cgtgcagcgc
      121 tcgcaagacg gcacgcgcaa gctcttgctc gagttgttcg acggcgggcg ggtcgagtgc
      181 gtgctgatcc cgatgacgcg gctcgacgcg gagacggacg ccgacgccgc ggccgcggtg
      241 gacgacgagc ccgaccccga agaggcggag ccgaagacca aggtcacgct ctgcatctcg
      301 actcagtacg gctgcgcgat gggctgtcgc ttctgcgcga gcgggcgcgc ggggcttttg
      361 cgcggcttga aagccgccga aatcgtcgcg caggtcttgg tgtccaagcg ctacctcgag
      421 ccgaatgaag agctcaagaa tctcgtgttc atggggatgg gcgaaccgct gcaccactac
      481 gacgaaacgg cgcgcgcgct tcgcttgttg atgaacacgg agggcctcaa catgagcccg
      541 cggcgcatca ccgtgagcac ggtgggcctc gttcccggca ttcaaaagct cgggcgcgac
      601 ttcgacggca agatcgggct cgcggtgtcg cttcacgccc cgaacgacca catccgaagc
      661 agcatcatgc cgatgaacga tcgctttccg atcgccgagc tgatgcaagc gctgcgcgcg
      721 tatccgttgc ctcgtcgccg ccgcatcacc atcgaataca cgttgataga cggcgtgaac
      781 gattcgcgcg agcacgccga cgagctcgtt tggctacttc ggcgcttgcc ggtgaagatc
      841 aacctgatcc cgatgaatcc ggtcgaaggg tcgggctacc gagctccgcc cgccgcgcgc
      901 gtgagcgaat tccagcgcgt gctcgcggac gcggggtaca gttgtttcgt gcgcacccgg
      961 cgcggagacg aggtcgatgc agcgtgcggg caactcgcgt tgtctcccga cgtcgtcgag
     1021 ctgcgcaaaa agccaagcgc ctgaagcaac catgtttcga aaaatcttgg tcttgaaccg
     1081 cggcgagatc gcttgccgta tccttcgcac gtgccgtcgg ctgggcgtcg cgaccgtcgc
     1141 cgtgtactcc gacgcggacg cggcgttgcc ccacgtaaag caagcagaca gcgcggtgcg
     1201 gctcggtccg gcgccggtca aagacagcta cctgaacctc gacgccatcg tcgaggccat
     1261 ccggagctcc ggcgccgaag cggtgcaccc gggctacggc cttttgagcg agagcccacg
     1321 gctcgctcgt gccgtcgccg atacgggtgc cgtgttcatc gggccgaagc cagagattct
     1381 cgagcgcctc ggcgacaaga tctcggcgcg cgcgctggct cgctcggtcg gggtggagcc
     1441 accgcccggc tctgcgtcgc cgctcgatcc gagcgacgcg gaggcggtcg cggcagaggc
     1501 cgagcgcgtc ggttatccgg tgatcgtcaa ggcggcggct ggcggcggcg gcatcggcat
     1561 gctcgtcgtg cgtgaagctt ccgagctcgc gcgcgccgtg aagacctgca cggatcgggc
     1621 gcgccaggcg ttttcggacg accgcgttta cctcgagcgc tacctcgaac gtccccgcca
     1681 catcgaggtg cagatcctcg tcgatcgcgc gggcaacggc gtcgccctcg gcgaacgcga
     1741 gtgcagcgtg cagcgccgcc atcagaaggt gctcgaagag tcgccgagcc ccgcgagctt
     1801 cttcacgggg gaagcggggg aacaacgccg ccaggcgctg ttcgcaaagg ctctgcgcgt
     1861 cgttcacgcc gcgaactacg aaggcgccgg tacggtcgag ttcgtctgcg acgcgagcgg
     1921 cagcgcctac ttcttggaag tgaacgcgcg ccttcaggtc gagcaccccg tgactgaaat
     1981 ggtgactggc ctcgatctcg tcgagcagca gctctgcatt gcctgcgggg aagagctgcg
     2041 ccacgacgtt caggccgtgg cgaggaatgg gcacgccgtc gaagtgcgca tttatgccga
     2101 ggatccgagc aagaatttct tgccgcaacc agggcggatc gaaacctttg cgtttcccat
     2161 gacgttcgaa ggagtacgca tcgagagtgg tttcgaggcg ggcgcggaag tcacccccta
     2221 ttacgacccg ctcctcgcca agctcatcgc ccacggcgcg acccgcgaaa ttgcgctcga
     2281 gcgcctcgac gccgcgctcg aagccacgca gatcgcgctc gtcggtccca aaggcccgcg
     2341 cgcgacgaac ctcgcttggc tacgtcaggt gctcgcggag cccatgttgc gcactggcga
     2401 ctacgatacg ggacttgccg atcgggtcgg agcgcgttcg tgagcgcaga gctcatcgag
     2461 cgggcccgcg cttgggtcgc cgcggatccc gatccggcga ctcggcgaga gctgctcgac
     2521 ctcatcgacg cgggcgcgaa cgcagaaatt tcggagcgct ttcaggccga actcgacttc
     2581 ggaacggcgg ggttgcgcgg cgaagtcggg gcaggcccgg cccggatgaa tcgcgctgtc
     2641 gtcatgcgcg cggtgcgggc actggctgag catttactcg cgcaaggggc cgaggtgcgc
     2701 tcgcggccgg tggtggtcgg gtacgacgcg cgcccgtcga gccagagctt tgccgaggcc
     2761 gcgacgggtg tgttgctcgc cgcggggatt gcggtgcgct ggttcgaaac gccggtgccg
     2821 acgcccctcg tctgctttgc ggcgcgtgtg tacggcgcgg cggcggccgt ggtcgtcacg
     2881 gcgagccaca acccgagccg cgataatggc ctcaagattt acggcgggga cgcgcgccag
     2941 ttggtcgcgc ccgaagacgt cgacgtcgcg cggcgtcgtg acgcggtcgg tcccgcggcg
     3001 gcaatccctt gtgtcgactt cgcgcgcgcc gagggccgcc tcgattcgat ccctgaagcc
     3061 atcgtctcgc agtacttcga cgaggtcgag gcgctgctgc cgccgcgttc ggcagagcca
     3121 gcgttgcgca tcgtttatac gcccgttcac ggcgtggggc tcgagctcgc ccgcgctggg
     3181 ctctcgcgcc gaggctttcg cgacgtgcac gtcgtcgccg agcaggcggc gccggacggc
     3241 accttcccga ccgcgccgtt tcccaatcca gagcttccgg gggtgctcga ccgcgcgctc
     3301 gagctcgcga agcgagtgaa cgcggacgtc gtcgtggcaa acgaccccga cgcggaccgg
     3361 ctggcggcgg cggctcgaac gcgggacggc gcctggcagg tgctgagcgg caacgaaata
     3421 gcggttttac tcgccgattg cgtgctcgag cgcgctccca aagcgccgcg accattgctc
     3481 gttacgtccg tcgtttcgac gccgctcctc agcgatgtcg cgcgaacgca cggtgctcac
     3541 tgcgagcgaa cactcactgg cttcaaatgg atttggcacg ccgcgcgctc actcgaggca
     3601 gagggcgggc tcgcctacgc gatgggatgc gaagaggcg
//
LOCUS       DASTKS010000009         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_669706, whole genome shotgun sequence.
ACCESSION   DASTKS010000009 DASTKS010000000
VERSION     DASTKS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669706"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..955)
                     /locus_tag="VFQ35_00155"
     CDS             complement(221..955)
                     /locus_tag="VFQ35_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929061.1"
                     /translation="MALIGGTAHAREASPHASRSSEVRRATPPAKSSDKARRSSKAQP
                     RPKPTPPKPSSEATFVTEGWAIERRFDADLDGDGARDRVLVVLQHDADKTEFRRRALV
                     ILLRREGGYILGGQNDKLINCLDCGGVRGGDGSPQLEIQRGSLVVTQYIGSREYSSVT
                     SRFRWSAERRRFELIGEDKTQGDALTGESLTTSCNLLTFACIETKTPPQVDDQGNDRR
                     AASKRRSHPLPRKPPTPLEEVTAPDW"
     gene            complement(1091..1480)
                     /locus_tag="VFQ35_00160"
     CDS             complement(1091..1480)
                     /locus_tag="VFQ35_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929062.1"
                     /translation="MSRFNQRRGEVAERVAERRRREEAAPRLTERVPKLESLKFDVQE
                     LRAGAIIPESTHVRRIPVPHAAALFEFPCLDSFCKDGGHDLTHSILRQLESRATTFEV
                     EDACGGQTGNASCQRVLRVVAHATYTS"
     gene            complement(1491..1943)
                     /locus_tag="VFQ35_00165"
     CDS             complement(1491..1943)
                     /locus_tag="VFQ35_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929063.1"
                     /translation="MSNDAVGAPDSSTVTGEQGAPARESGAAMDPTTANPERVAPATA
                     PSAPKAPRQSSGTKPAARKISAALQRAVQGAGEAWAVDSANALRDEGRQVTGGWPGTI
                     SEARQRVRTCALLQGVCMTSEDLEVLTRVAYETAKKVWLARSERVSDD"
     gene            complement(1940..2308)
                     /locus_tag="VFQ35_00170"
     CDS             complement(1940..2308)
                     /locus_tag="VFQ35_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9929064.1"
                     /translation="MNNRLYVGNLAFHTTEDSLQRAFSQHGQVIETKVVLDRETGRSR
                     GFGFVVMGTADEAKKAIAEMDGTDLDGRPLRVNEAEERKPRAGGGGGGGFGGGGGGGG
                     FGGGGRGGGGRDRNDRGNRW"
     gene            3076..>5176
                     /locus_tag="VFQ35_00175"
     CDS             3076..>5176
                     /locus_tag="VFQ35_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929065.1"
                     /translation="MDLERHVGLRSDGTVVVRDRRTLSRSIALKLTAMGFRVPDSVAD
                     QELRDLERDLFARYREQMRLLSDHLCPADRRIQAFLDSLFARLALPYPIRLPSSTFIL
                     DRYGLARELSLPIDANEWHNEIISSYRLDNGVLHNPLNDRRTTQGVFHVADVGFPVPG
                     DKIGVPLVAYAHLLYHALHAPNHLLRLPFSAEWDEPAETMVSLLLRPLVCPAVPGVSP
                     EKRLEVRFFAPGGLVSNLDFLESIFGNSGDPFLPENDAGLDVEHWTGHTGCVILAPHL
                     TRLRKKDLGLPHASEASEAQKRDGMCWADPTELYNGGSPFKITARSMDGVMVTILADN
                     YFGYCKKEVKTQISFSANLYGLAEEEHAGGALAFATVSLGDAFAPERLAQSDHRFAEV
                     QELLGDSITLHPTGYATDKQYPEIHYLPEDMEIDLKRQDIKWTSRGEERHLKLLPGQI
                     YVHPSGYKVRMEKHPAAPSWRLIGTAAEGTFCHKPCTVSGGGKSEISKSLIDAVIYGP
                     IYVQNFEEDMAQVQAIFQKDYRQALRPELRGAVDNSRPLLSRDRSLGSVIKLLTPNPD
                     FTAEYNAWLEGVPNYIRALVFVIKRFHRPDWGNDWRKHFTVDIINGAPGHELKQDTRK
                     LVGSYLRVGLLGSGAWRTYKLRQDFIAADKVQMEDDITASVVVPAKALLGLPSEYDGH
                     PSLKLSENCEFRLFQRPD"
BASE COUNT          873 a         1698 c         1683 g          922 t
ORIGIN      
        1 aggggcgcgc tgaccgagat cggcgcgcgg tgcgctcgtg tccgtggaca caggcgattg
       61 aatcgtcggt gaacagagaa ggttggtgat gggatcccag agcgcggggg ttcgctcgtg
      121 tgcgcagcct tcgcgccttg ggacggtgag tgggacgaag cgccgctggg tgcgggaaaa
      181 tggtgggagg acccagcggc gcttccgagt ttcacaccct tcaccaatcg ggcgcggtca
      241 cctcttcgag cggtgtcggg ggcttccgcg gtagcgggtg agaacgtcgc ttggaggcgg
      301 cgcggcgatc gttgccttgg tcgtcgacct gcggcggagt cttcgtttcg atgcacgcga
      361 acgtgagcaa gttgcagctg gtcgtcagcg actctccggt cagcgcatct ccttgcgtct
      421 tgtcctcgcc gatgagctcg aagcggcgcc gctcggcgct ccagcgaaag cggctcgtca
      481 ccgacgagta ttcgcgcgag ccgatgtact gcgtcaccac cagtgagccg cgctggatct
      541 cgagctgtgg agagccgtcc ccgccgcgta cgcctccgca gtcgaggcag ttgatcagct
      601 tgtcgttctg cccgccgagg atgtagccgc cctcccggcg caaaaggatg acgagcgcgc
      661 gccttcgaaa ctcggtcttg tccgcgtcgt gctgcaacac gacgagtacc cgatcgcgag
      721 ctccgtcacc atccaggtcg gcatcgaagc ggcgctcgat cgcccaacct tcggtgacga
      781 acgttgcctc actgctcggc ttcggcggcg tgggcttcgg ccgaggctgg gccttggaag
      841 agcgccgcgc cttgtcgcta gacttcgcgg gaggcgtcgc gcgacgcact tcgctgcttc
      901 gggacgcgtg agggctcgct tcgcgcgcgt gcgcggtacc accgatcagc gccacgaacg
      961 ctacaacggt ccaagccgaa cgagtccgac ggctgtccac gacggatgcc cctgagctcc
     1021 ggaaagttct gctgcgacac tcgagcgccg aagcggcgaa ccagtgtcgc cgccgggacc
     1081 aaagcgccgc tcagctcgtg taagtcgcgt gcgcgacgac gcgcagcacg cgctggcacg
     1141 aggcgttgcc ggtctgaccg ccgcacgcgt cttcgacctc gaaggtcgtc gcgcgactct
     1201 cgagctggcg caaaatcgag tgagtcagat cgtgcccgcc gtctttgcaa aagctatcca
     1261 ggcacggaaa ctcgaaaagc gccgccgcgt gcggaaccgg gatgcggcgg acgtgagtgg
     1321 actctggaat gatggcacca gcgcgcagct cttgaacgtc gaacttcagg ctctcgagct
     1381 tgggcacgcg ctccgtgagg cgcggagcgg cttcttccct acgtcgccgt tcggcgactc
     1441 gctctgcgac ctcgcccctg cgctgattga atcgactcac gagactcctc ctagtcgtcg
     1501 ctgacgcgtt cgcttcgggc cagccacacc ttcttggctg tctcgtacgc gactcgagtc
     1561 aggacttcca aatcttcgct ggtcatgcaa acgccctgca agagcgcgca cgtgcggacg
     1621 cgctgacgcg cctcgctgat ggttcccggc cagccacccg ttacctggcg cccctcgtca
     1681 cggagagcgt ttgccgagtc gacggcccac gcctcaccgg cgccttgcac cgcacgctgg
     1741 agcgccgcac tgatcttgcg cgcggcaggc ttcgtcccgc tcgactggcg cggcgctttt
     1801 ggagcgctgg gcgccgtagc aggagcgact cgctccggat tcgcggttgt tgggtccatg
     1861 gctgcgcccg actcgcgtgc gggtgcgcct tgctcacccg tgacagtgga agaatcgggc
     1921 gcacccacgg cgtcgttgct caccagcggt ttccgcggtc gttgcggtca cggccgccac
     1981 cgccgcgccc accaccgccg aaaccaccac cgccgccgcc gccgccgaag ccgccaccac
     2041 cgccgccacc tgctcggggc ttgcgctctt cagcctcgtt cacgcggagg gggcgcccgt
     2101 cgaggtcggt cccgtccatc tcggcgatgg ctttcttcgc ctcgtcagca gtgcccatca
     2161 cgacgaagcc gaagccgcgc gaacggccgg tctcgcggtc caggacgacc ttggtctcga
     2221 tgacctggcc gtgctgagag aaggcgcgct ggagagagtc ctcggtggtg tggaacgcca
     2281 ggtttcctac gtacaatcga ttgttcatgg ctttttctca atcgcggtaa acggaccctt
     2341 tggtccctgt gaccgacact cgagaaaacc gcggaatcga aagtcgggtg cagcgaaaac
     2401 cgccgccaca ttgccgctgc cggacaagcg actttccgcc ctctcgaagc gagagcgcag
     2461 ccaggcgtta ccgaaaacga tgtgggcgaa gcttttagcc cctgggaggc gtggacgcaa
     2521 gcatcgggct ggtttcgtgc gctgaaacga cgggcacata gcgaccgcgc gtgaatgccg
     2581 ttatccgccg cccgcgcttc caaaacccga tggcgctcga tgtggggccg gtcgaccggt
     2641 agcggtgctg agcctcagcg gaatgtcggg ccgcaagatc gcgctcgggg cgcgatggtt
     2701 gcgaggcgga gtgctggacg aagtatcgcg ctcgggtcgc gacggttgcg gggcggagtg
     2761 ctcgacgaag cgcggtcgag gcgcgccgtc gtcgcggagt gaggacgacg gcagcgcgcc
     2821 tgtggtgagc gagactctgg tcggcgtcgg cgaaactcca ccgactggcg gagaggtcac
     2881 gagaagggtg ccgccgcgcg cagctgaaac tctgacacgt atgcaagtca ctcacactct
     2941 tcggagttca agtgagtgca aattggcgcg acgtttctcg tcgagtcgca cgagcagcgt
     3001 agcgcgctgt ccgtcgttcg ccggctcttc tcgcacgcgt gcgtccatgt tgagctcctg
     3061 cgcgaggtgc tgggaatgga tctcgaacgt cacgttgggc tgcgctcgga cggaactgtc
     3121 gtagtgcgtg atcggcgcac gctgtcgcgc tcgatcgcgc tgaagctgac tgccatgggc
     3181 tttcgagtgc cggacagcgt cgcggatcag gagctgcgcg atctcgagcg cgacctgttc
     3241 gctcgctacc gagagcagat gcgtttgctc tcggatcacc tgtgccccgc ggatcggcga
     3301 attcaggcgt tcctcgattc tctgttcgcg cggctcgcgc tgccgtaccc gattcgtctg
     3361 ccatcctcga ccttcatttt ggatcgctac gggctcgcgc gagagctctc tttgcccatc
     3421 gacgcgaacg agtggcataa cgagatcatc agcagctacc gcctcgacaa cggcgtgctg
     3481 cacaacccgc tcaacgaccg ccgcacgacg cagggagtgt tccacgtcgc agacgtcggc
     3541 ttcccggttc ccggcgacaa gattggggtg ccgctcgtcg cgtacgcgca cctgctctac
     3601 cacgcgctgc acgccccgaa tcatctgctc cgcttgccgt tctcggcgga gtgggacgag
     3661 cctgccgaga cgatggtatc gcttctctta cggcctctcg tctgcccagc ggtgcccggg
     3721 gtttccccag aaaagcggct cgaggtgcgc ttcttcgcgc cgggcggctt ggtctcgaac
     3781 ctcgacttcc tggagagcat cttcggcaac tcgggcgatc cgttcttgcc cgagaacgac
     3841 gcggggctcg acgtcgagca ttggacgggg cacaccggct gcgtcatctt ggcgccgcac
     3901 ctcacgcgtt tgcgcaagaa ggacctcggg ctgccgcacg cctccgaggc gagcgaggcg
     3961 cagaagcgcg atggcatgtg ctgggccgat ccgaccgagc tctacaatgg cggtagcccc
     4021 ttcaagatca ccgcgcgcag catggacggc gtgatggtga cgatcctcgc cgacaactac
     4081 tttggttatt gcaagaagga ggtgaagacg cagataagct tcagcgcaaa cctctacggt
     4141 ctcgccgagg aggagcatgc gggcggcgcg ctcgccttcg ccacggtgag tctcggtgac
     4201 gccttcgctc ccgagcggct cgcccaatcg gatcatcgct tcgccgaggt ccaagagctg
     4261 ctcggcgaca gcatcacgtt gcacccgacg ggctacgcga ccgacaagca gtatccagag
     4321 attcactact tgccggagga catggagatc gacctcaagc ggcaagacat caagtggacg
     4381 agccgcggtg aagagcgcca cttgaagctc cttcccggcc aaatctacgt gcacccgtcc
     4441 ggatacaagg tccggatgga gaagcacccc gccgcgccga gctggcgcct catcggtacc
     4501 gccgccgagg gcaccttctg ccacaagccg tgcacggtct cgggaggagg gaagtccgag
     4561 atcagcaaga gcctgattga cgccgtgatt tacggcccca tttacgtgca gaacttcgaa
     4621 gaggacatgg cccaggtcca ggccatcttc cagaaggact atcggcaagc gcttcgcccg
     4681 gagcttcgcg gcgccgtcga caactcgcgt ccgctgctct cgcgcgatcg ttcgctcggc
     4741 tcggtcatca agctgctcac gccgaacccc gacttcaccg ccgagtacaa cgcgtggctc
     4801 gagggagtgc cgaattacat ccgagcgctc gtgttcgtga tcaagcgctt tcatcgcccg
     4861 gactggggta acgattggcg caagcacttc accgtcgaca tcatcaacgg agcccctggg
     4921 cacgagctca agcaagacac gcgcaagctc gtcggaagct acctgcgcgt cgggctgctc
     4981 gggagcggcg cgtggcgtac gtacaagctc cggcaggact tcatcgccgc cgacaaggta
     5041 caaatggagg acgacatcac cgcctccgtg gtcgttcccg cgaaggcgct cttggggttg
     5101 ccgtcggagt acgacgggca cccgagcttg aagctcagcg aaaactgcga gttcaggctc
     5161 tttcagcgtc ccgacg
//
LOCUS       DASTKS010000010         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_669848, whole genome shotgun sequence.
ACCESSION   DASTKS010000010 DASTKS010000000
VERSION     DASTKS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669848"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1564)
                     /locus_tag="VFQ35_00180"
     CDS             complement(20..1564)
                     /locus_tag="VFQ35_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929066.1"
                     /translation="MLKRLACGIVCSYLVGCAVPTVEGGNNGNDRSNGGEQGEELLTG
                     AMIVSPDGKWAVMQRNQTSVLLDIEQMKPREMPSQVARFVFAETGEHGVALLMDRTLV
                     DYDLGTLAVRWKRALPFATPSMPSLLRLSPDGKQLLLADGPTLFVLDAASATLQKQLE
                     LRSPAAELTFVPDKRRALVVGSTRWSEHLPSTEVAEVDLEAFTLASIDVPNCTAPIAV
                     LPDASRALLSPTFCEEGVASTGQQTWTNPDPVSIVDLAAGGPSFVKNLPGFGPVALET
                     SGKYAVAYLDVKRMDASMFDDPAEVPSLTGPRYHVMRIDPKTLDFALFPIGEVLPRFA
                     LGRDGKTLLVDATVQQYRGQASVKASIDSSGHLTASFSVFGKVDSLFGALDLSSGTYA
                     PFGGSTAALDRFVQTRDGKRVFTLKLRADGSGGDLYRIDLDQGSTTALNKSLRDIGLL
                     ADGETLLLRERLPAAQITVGATVSWYRRERYCLSLDGLTCSVSVDFQDSTPFQSGPTC
                     TDYHDC"
     gene            1809..>2095
                     /locus_tag="VFQ35_00185"
     CDS             1809..>2095
                     /locus_tag="VFQ35_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9929067.1"
                     /translation="MAQLSGFKVAILVTEGFEQVELEQPKKALDEAGADTRIVSPAKE
                     HVRAWKHFDKADTFGVDVTLDEASAEDFDALLLPGGVANPDQLRALPKAVK"
BASE COUNT          400 a          655 c          680 g          360 t
ORIGIN      
        1 cgcgcggatc ggctcgggct caacagtcgt ggtagtcggt gcacgtcggt ccgctttgga
       61 acggcgtgct gtcttggaag tctacggaga cgctgcaagt gaggccgtcg agggagaggc
      121 agtagcgctc acggcggtac caagagacgg ttgcgcccac ggtgatctgc gcggcgggca
      181 agcgctctcg cagcagcagc gtctctccgt cggcgagcaa accgatgtcg cgcaggctct
      241 tgttcagcgc ggtcgtcgag ccctggtcga gatcgatgcg atagagatct ccgccgctcc
      301 cgtccgcgcg gagcttgagc gtgaacacgc gctttccatc tcgggtttgt acgaagcgat
      361 cgagcgccgc ggtcgagccg ccaaacggcg cgtacgtccc gctcgatagg tcgagcgcac
      421 cgaagagcga gtcgaccttg ccgaagacgc tgaagcttgc cgtaagatgc ccgctgctgt
      481 cgatcgacgc cttcacgctc gcttgacccc ggtactgctg caccgtcgcg tcgacgagga
      541 gcgtcttgcc gtcccgaccc agcgcgaagc gcggcaggac ctcgccgata ggaaacagag
      601 caaaatccag agtcttcggg tcgatgcgca tgacgtggta gcgcggcccc gtgagcgacg
      661 gcacttcggc agggtcgtcg aacatcgacg catccatgcg cttcacgtcg aggtaggcga
      721 ccgcgtattt tccactcgtt tctagtgcaa ccgggccgaa gccgggcagg ttcttcacga
      781 aggaggggcc ccccgcagcg agatcgacga tgctgaccgg gtccgggttc gtccacgttt
      841 gctgccctgt gctcgcgaca ccctcttcgc aaaacgtagg agagagcaac gcacgcgacg
      901 cgtcggggag taccgcgatc ggagccgtgc agttgggaac gtcgatgctc gccagcgtga
      961 acgcttcgag gtctacctcc gcgacttcgg tggacggcag atgctcgctc caacgagtgg
     1021 aaccgacgac gagagcgcgt ctcttgtcgg ggacgaaagt gagctccgcg gctggggagc
     1081 ggagctccaa ctgcttttgc aacgtcgccg acgccgcgtc caacacgaag agagtcgggc
     1141 cgtccgcgag cagcagctgt ttgccatcgg ggctcaacct gagcagactc ggcatcgacg
     1201 gagtcgcgaa cggcaacgcc cgcttccagc gcactgcgag tgttccaaga tcatagtcga
     1261 cgagcgtacg atccatgagc agcgcgaccc catgctcgcc ggtctctgca aaaacgaaac
     1321 gcgcgacctg cgagggcatt tcacgcggct tcatctgctc gatgtcgagc agcaccgagg
     1381 tctggttgcg ctgcatgacg gcccacttcc cgtccggcga gacgatcatc gcgccggtaa
     1441 ggagctcctc tccttgttcg cccccgttac tgcggtcgtt gccgttgtta ccaccctcga
     1501 cggttggaac ggcgcagccc actaggtacg aacagacgat tccacaagcg agtctcttaa
     1561 gcatgctcgc cacagaagca gagagcggac caacgtacgt tcgaacgcat tttcaggtgt
     1621 aagcctggat ttcccggagg aatcgacgtg atccggatgt catacgagaa ccatcgaacc
     1681 cagtgtggcc acctcgcctc agggtcgcgc gtggggttgg gcgcgcgaag ctctggtcgc
     1741 ggcatggcgg ttgcacccaa tgtggccgcg tcgcgcacac acgcggcgct gaaaggacac
     1801 ttggttccat ggcccagctc agcggattca aagtagcaat tctcgtcacg gaggggttcg
     1861 agcaggtcga gctcgaacaa cccaagaagg ccctcgacga ggcgggcgca gacacgcgca
     1921 tcgtgtcgcc ggcaaaagag cacgtgcgcg cctggaagca cttcgacaaa gccgacacgt
     1981 tcggcgtcga cgtcacgctc gacgaggcaa gcgccgagga cttcgatgcg ttgttgcttc
     2041 cgggcggcgt ggcgaacccc gatcagctgc gcgcactgcc gaaggcggtg aagtt
//
LOCUS       DASTKS010000011         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1115961, whole genome shotgun sequence.
ACCESSION   DASTKS010000011 DASTKS010000000
VERSION     DASTKS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115961"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1787)
                     /locus_tag="VFQ35_00190"
     CDS             complement(195..1787)
                     /locus_tag="VFQ35_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929068.1"
                     /translation="MRRGAGTAAPSLALCLAAFVGCSLMALDDFDAKPCVTDADCALA
                     NANYLGGRCGHYSCVAGLCTPPDGVERCGRGDEDCDGLIDEGDDLVTPVESGGAAEPV
                     PTIAWAFARNPPSSLLVVPGDEALGVTLDEGFARIGAVQPLTFSRDGDPTPCRNHNGQ
                     SACNAEEVALAVDDEHAVFAAINTLGCAAGQVRLAIGERSDPFSLLVDKSPESLSPAE
                     SNLALGVDTDEKDRCTGASRAADAEGVVARGATRPAIAVNSTASGASGALALWLAAPR
                     DANQSSVSGCASHDPITVEGIGVFVPLGEPPWLQGTDGGKPTSFGTTTSLSTPAVLAL
                     GESQGGGDYLVAFAGERDSRPGIVFVRAHVQGAHLSVSLDPLFIADDAPEQVTLSLAG
                     THAGQTEIGVAWSSGCGAGRMLRFTTRALETAASGREIAAFAAPNLASSAQLLHFDTG
                     FATSAPTGGWALTWLDAPATGPSSVRFARIRDTTLQKLNDVTLRTGAVGVPLMFPGGD
                     SGVRFVFVHDVNRIVSAPGWCP"
     gene            complement(1787..2680)
                     /locus_tag="VFQ35_00195"
     CDS             complement(1787..2680)
                     /locus_tag="VFQ35_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929069.1"
                     /translation="MAEDGQVDPRLARARVYAATAEGLFDQGHYAAALAEYTRVYEAL
                     AGHPRQYWVLYNLAACHERLFQYDLALARYEEYLTRAPESEKDRAEVAAITRTLRSLL
                     ATLVVQSSVPSEVWVDDRRMGTTPGHWLVGAGRHIVEVRAELYGSERREVQLKSGSSE
                     LLRFELQRLSTYAGPGRGFFWASAGLSAAAVIAGSAFGMMALSAHRDGRDRARASLDT
                     SAEARRARDWSLAADVGFGSAILFGATATVLYFVTDWRQPTTDRSRTAARSRRSAMLR
                     PRAELDFSPSGRAAATLAWEF"
     gene            complement(2865..>4125)
                     /locus_tag="VFQ35_00200"
     CDS             complement(2865..>4125)
                     /locus_tag="VFQ35_00200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929070.1"
                     /translation="VATARRFPVDRALNVLVQLCDALSAVHAAGIVHRDLKPDNIFLV
                     ERRGQPDFVKVLDFGVCTFAASDAERLTASGDAVGTPLFMAPEQLEARHAVDERADIY
                     ALGTILFFMLTGEAPFSGSTLLALGSKICHAPPPSLASRGCEAPPELEAILQKALRKK
                     PEARFQTCAELKSAIEEFLGPRSHVADTTLVSVPAPRIVLEESPDPHESTAIPRLAMD
                     RSNLIFTAGLGLVAVAGAAAALVHFVRSDPFPKAIVDGRAGNTSVARIASLHASKAAA
                     SASAGVPSAAPSGPSGSPVTAAVGSSDLSSNALPAGPTAELAASSTSVADAAPAASAP
                     AARMRPPASGALSSASGRSSKAERTAAEKRLPAAEESPVSAASTGSVPKATDETKSSA
                     KGDTSSPRDVPPNPNGVSLNHGPKRGL"
BASE COUNT          719 a         1371 c         1377 g          658 t
ORIGIN      
        1 cggctctacc accgtgggct gcagcgctgc ccccggtacc cgaagggact ccgtcgtcat
       61 cggcacagcc cgagagcgag ccgagcacca gccccaccaa cacggcggcg cggagtcgag
      121 gcagtcttcc tagccaattc atcacagagc tcccttttat gtcgggtttt gaaaatttcc
      181 catttcagac agtgtcaggg gcaccatccg ggcgctgaaa caatgcggtt cacgtcgtga
      241 acgaacacga accgcacgcc gctgtctcca cccggaaaca tcagcggaac gccgactgct
      301 ccggtgcgca gtgtcacgtc gttcaatttt tggagtgtgg tgtcgcggat ccgcgcaaac
      361 ctgacactcg acgggcccgt tgcgggcgca tcgagccacg tcagagccca gcccccggtg
      421 ggcgccgacg tcgcgaagcc ggtgtcgaag tgcaagagtt gggccgacga ggcgaggttt
      481 ggagcggcaa atgccgctat ttcgcggccc gatgcggccg tttcgagcgc gcgcgtcgtg
      541 aagcggagca ttcttccagc gccgcagcct gagctccacg ccacgccgat ctccgtttgg
      601 ccggcgtgcg tgcccgctag cgacaacgtc acctgctctg gagcgtcatc agcaatgaag
      661 agcggatcga gtgacaccga taggtgcgcg ccctgaacgt gcgctcggac gaatacgatc
      721 ccgggccgag agtcgcgttc acccgcaaac gcgacgaggt agtctccccc gccctgcgac
      781 tcgcccaacg cgaggaccgc cggcgtcgac aaactcgtcg tggtgccgaa cgacgtcggc
      841 ttcccaccgt cggtgccctg aagccacggc ggttcgccga gcggaacgaa cacgccgatc
      901 ccttccaccg tgatcggatc gtgcgacgcg catccgctca cgctcgactg gttcgcatct
      961 cggggagccg caagccaaag agcgagagcg ccgctggcgc ccgacgccgt gctgttgacc
     1021 gctatcgcgg gccgcgtcgc gccgcgtgcg acgacgccct ctgcgtccgc ggcgcgagaa
     1081 gctccggtgc agcggtcttt ttcgtcggtg tcgacgccga gcgcaaggtt gctctctgcc
     1141 ggcgagagcg actcgggtga cttgtcgacg agaagtgaga acggatcgct gcgttcgccg
     1201 atcgcgagcc gtacctgtcc cgctgcgcaa cccagagtat tgatggctgc gaaaacggcg
     1261 tgctcgtcgt ccaccgcgag cgcaacctcc tcggcgttgc aagcgctctg gccgttgtga
     1321 tttcggcacg gggtggggtc gccgtcgcgc gagaacgtga gcggttgcac ggcgccgatg
     1381 cgagcgaaac cctcatcgag cgtcacgcca agagcttcgt cgcctggcac gaccaacagc
     1441 gaagaaggag gatttcgcgc gaaggcccaa gcgatggtcg ggaccggctc ggccgcgcca
     1501 ccactctcga ccggggtcac gaggtcgtcg ccttcgtcga tcaacccgtc gcaatcttcg
     1561 tcaccgcgtc cgcagcgttc gactccatcc ggcggagtgc acagcccggc aacgcacgaa
     1621 taatgcccgc agcgaccgcc gaggtagttg gcgttcgcca aagcgcaatc agcgtctgtg
     1681 acacagggtt tggcgtcgaa atcgtcgagc gccatcaagc tgcagcccac gaatgcagcg
     1741 aggcaaagcg cgaggctcgg agcggctgtt ccggctccgc gtcgcatcag aactcccatg
     1801 cgagagtcgc cgccgcgcgg cccgatggac tgaaatcgag ctccgcgcga ggtcggagca
     1861 tcgcgcttcg tcgtgaacga gccgccgtgc gcgagcggtc cgtcgtaggc tgcctccagt
     1921 ccgtcacgaa gtagagaacg gttgccgtag cgccgaacaa gatcgcgctc ccgaagccga
     1981 cgtccgccgc gagcgaccag tcgcgcgccc ttcgtgcctc tgcgctcgtg tcgagcgatg
     2041 cgcgcgcgcg atcgcgccca tcgcgatgcg cggagagcgc catcattccg aacgcgctgc
     2101 ccgcaatcac ggccgcggcg ctgagaccag ccgaggccca aaagaaacct cggccagggc
     2161 cggcgtaagt cgagaggcgc tgtagttcga agcgcagcaa ttcgctgctc ccgctcttga
     2221 gttgcacctc gcgccgttca gagccgtaga gttcggcgcg cacttcgacg atgtgtcgac
     2281 ccgctcccac gagccagtgc cccggcgtcg tccccattct gcggtcgtcg acccagactt
     2341 cgctcggcac cgaactctgc acgaccagag tcgcgagcag cgagcgcagc gtccgggtga
     2401 tcgcggccac ttcggctcga tctttttcac tctctggcgc gcgcgtcagg tactcctcgt
     2461 aacgcgcgag cgcgagatcg tactggaaaa ggcgctcgtg gcaggctgcc aagttgtaaa
     2521 ggacccaata ttggcgcggg tgccccgcca gggcctcgta aacgcgcgtg tactcggcga
     2581 gagccgccgc atagtgacct tgatcgaaga gcccttcggc cgtcgcggcg taaacgcgcg
     2641 cgcgagcgag tcgtggatcc acctgcccgt cctcggccat ttcactgctc cgagcgggct
     2701 ccgcgtgagc tcccggaatc gggccaacgc aagcgcacgc gacgagcacg gcaactgcct
     2761 tcgtagtgaa agcgcggagc attcggccgc ccgtggagcc ccagacggtt gaagctctcc
     2821 atgaaccgag agtgaaacgc cgcgctgtca aatggatcgg cgttctaaag accacgcttg
     2881 ggaccgtggt tcaaggagac accgttgggg ttcggcggaa cgtcgcgcgg ggatgacgta
     2941 tcaccctttg cggatgactt cgtttcgtca gtcgccttcg gcacggagcc ggtggacgcg
     3001 gccgaaaccg gcgactcctc ggcagccggg agccgcttct cggcggcggt tcgctccgcc
     3061 ttgctggaac gacctgacgc ggagctcagg gctcccgaag cgggtggccg catgcgagca
     3121 gccggtgcgg aggcagccgg tgctgcgtct gccacgctcg tcgacgacgc tgcgagctcg
     3181 gcggttgggc cagctggtag agcgttgctc gaaaggtcgc tcgagccgac tgcggctgtg
     3241 accgggctgc cagacgggcc ggacggagcg gcgcttggaa caccagccga tgcggacgca
     3301 gccgccttcg aggcgtgtaa gctggcgatg cgcgcgacgg acgtgttgcc cgctcgaccg
     3361 tcgacgattg ccttcgggaa cgggtcgctc cgcacgaaat gcacgagcgc agcggctgcg
     3421 ccggccacgg cgacgagtcc caggccggcc gtaaaaatga ggttcgaccg atccatcgca
     3481 aggcgcggaa ttgcggtgga ctcgtgcggg tccgggcttt cttcgagcac gatccgcggt
     3541 gccggcacgc tcaccagggt cgtgtccgct acgtgagagc gcggccccag gaactcttcg
     3601 atggcgctct tgagctcggc gcaagtctga aagcgcgctt cgggcttctt gcggagcgcc
     3661 ttctggagaa tggcttcgag ctcgggcggc gcttcgcagc cgcggctggc gaggctcggg
     3721 ggcggggcat ggcagatctt cgaccccagg gcgagcaggg tcgaaccgga gaacggcgct
     3781 tctcccgtga gcatgaagaa caagatcgtg ccgagcgcgt agatgtcggc ccgctcgtcg
     3841 acggcgtgcc gagcctcgag ctgttctggc gccatgaaca ggggtgtgcc caccgcgtcc
     3901 cccgaagcgg tgagccgctc ggcgtcagac gccgcaaagg tgcaaacccc gaaatcgagc
     3961 accttgacga agtctggctg cccgcgtcgc tctacgagga aaatattgtc gggcttgagg
     4021 tcacgatgca cgatcccggc cgcgtgaacc gccgaaagcg cgtcgcagag ctggaccagc
     4081 acattcaggg cgcggtccac ggggaaacgg cgcgcggtcg cgacc
//
LOCUS       DASTKS010000012         6422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_893067, whole genome shotgun sequence.
ACCESSION   DASTKS010000012 DASTKS010000000
VERSION     DASTKS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893067"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VFQ35_00205"
     CDS             complement(<1..1363)
                     /locus_tag="VFQ35_00205"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET9929071.1"
                     /translation="MSLKYFARPIDPNHYVLPKTGSMRVDAHAFFSEQLYEASEESMW
                     QQLASAASYEGVIGAYLMPDAHSGYGVPVGCVIVTEDTIIQAGSGYDISCGVLYMRVP
                     GLGAEAIADWDKRERWVREVEDRVATGVGSHRPKQMPHFSHRKAGEILRYGAKAIGVR
                     AELCERQFIEVDEAVDLTSIERAYEKVVPQLGSVGGGNHFIEMQVDRDTGDVWVMIHC
                     GSRGYGWQTANHFFYAGAELRGLPRNRREDSWLRIDEPLGREYWAHHNSAANFAVANR
                     HIIVNGVQDALREVFQREGEVYYEISHNLVQEETLVLPDGTTQRGFVHRKGATRAFPA
                     GHPDLRGTAWEKTGHPCLIPGSMYHGAAILFAEPGASKSGFSVNHGSGRLLARGAAKR
                     SLEASHDAIDREMREVLRTLGGVKVRGIVGNTERTPLDECGHVYKDLDVVLAVLEAEG
                     IARV"
     gene            complement(2213..3784)
                     /locus_tag="VFQ35_00210"
     CDS             complement(2213..3784)
                     /locus_tag="VFQ35_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HET9929072.1"
                     /translation="MALKTLFASATKALTAKGKNEAGGTAYEFTPSHALAQYAATGTF
                     NRTFYATEGEQLERVLELCRAVDPVFVAKTAVYARERARMKDMPAFLLAYLAAVDVRL
                     LSLVFDRVIDNGKMLRNFVQIVRSGVVGRKSFGTAPKRLVRTWFDKRTPAALFRQSIG
                     NDPSMKDVIKMLRPSPVVNGVADAEREALYGYLIGKDVAFERLPAVVQTFEAFKSGSP
                     EVPDVPFEMLTALPLSREMWMQIARRMSFTQLRMNLNTLLRHGVFEHRDMIDFAAARL
                     SDPDAVRKARVFPYQLLAAYTAAQATLPNELALALQLALEVSIENVPAVPGKVYVCPD
                     VSGSMQSPVTGHRKGSSSKVRCVDVAGLVAAAFLRRNQTAEVIPFSDDVVSMSRRLNP
                     LDSVMTNAAFLASLPSGGTACSAPLRHLNKHKAEGDLVIYVSDNQSWADFGVSQRATP
                     MAEEWERFRKRSPNAKLVLIDVQPYGSTQVSERADVLNVGGFSDSVFEVVSLFAKGEL
                     DAEHWVGEIEKVALA"
     gene            complement(4468..6132)
                     /gene="ggt"
                     /locus_tag="VFQ35_00215"
     CDS             complement(4468..6132)
                     /gene="ggt"
                     /locus_tag="VFQ35_00215"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9929073.1"
                     /translation="MLPAWFGPLAAAAGLVALSPRLQGAPLAERGAVASEHPLATRAA
                     LEVLKQGGNAVDAAIAAAFAAGVVSPTSSGLGGGGFAMVLRPNDAEPSILDFRETAPR
                     GVSVEALERRPLPYEERGHLIGVPGESAGLFELSRRFGKRAWATLAAPAVRLAGQGFP
                     VGPHLARSLAGNAELARDKSLAQLFFPQGRAALEGRVVKNLPLSRTLARVAAEGPKAI
                     YQGAIATELVETARSAGSALAQSDLDDYAVKERKALHVSFAGHEVYTMPPPSAGGLLL
                     AETLGMLSASELGRLGYQSGAYQHLLAEAARSAFADRFRYASDPDFTPVDTAKLLAPE
                     RLAAKRRALSLDRTHSLPLLVSEEHGTHHIVVADSTGMVVSLTTTVNRLFGAKLVAKN
                     SGIVLNDELDDFTLARDAKALGIAASPNAPRAGARPVSSMTPTIALKDGRPVLAIGGS
                     GGMAISTNVTQLVVARLVFGKSAEELTHQQRFFVPLKGDTIWPEASAPPSLFEDLAYR
                     GERAKKAPDFATAVQVLTRDGALWQASADPRKHGAAADSSGEHKPE"
     gene            6238..>6422
                     /locus_tag="VFQ35_00220"
     CDS             6238..>6422
                     /locus_tag="VFQ35_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET9929074.1"
                     /translation="MRLLFVMDPFNGLTFGKDTSFAFMRGAARRGHEVWHCLPEDVTS
                     RGRDVFARATRLSVNQAA"
BASE COUNT         1172 a         2081 c         2051 g         1118 t
ORIGIN      
        1 ccacgcgcgc gatgccctcg gcttcgagca cggcgagcac gacgtcgagg tccttgtaga
       61 cgtgaccgca ctcgtcgagc ggagtgcgct cggtgttgcc gacgataccg cggaccttca
      121 cgccgccgag cgtgcgcagg acctcacgca tttcgcgatc gatcgcgtcg tgcgacgcct
      181 cgagcgaacg ctttgccgcg ccgcgcgcga gcaagcgccc cgagccgtgg ttcacggaga
      241 agcccgactt cgatgcgcct ggctcggcga agaggatggc ggcgccgtgg tacatcgacc
      301 ccgggatgag gcagggatga cccgtctttt cccaagccgt accgcggagg tccgggtgtc
      361 ccgccggaaa cgcgcgcgtc gcacccttgc ggtgcacgaa gccgcgctgc gtggttccgt
      421 cgggtaacac gagcgtctct tcctggacga ggttgtgcga aatctcgtag tagacctcgc
      481 cttcgcgctg gaaaacctcg cgcaacgcgt cttgaacgcc gttcacgatg atgtgtcggt
      541 tcgcgacggc gaagttcgcg gcgctgttgt ggtgcgccca gtactcgcgc ccgagcggct
      601 cgtcgatgcg caaccacgag tcttcacgac ggttacgcgg caagccgcgc agctccgcgc
      661 cggcgtagaa gaaatggttc gcggtttgcc agccgtagcc gcgcgacccg caatggatca
      721 tcacccagac gtcgccggtg tcgcgatcca cctgcatttc gatgaagtgg ttacctccac
      781 cgacggatcc aagttgcggc accaccttct cgtaggcgcg ctcgatactg gtgaggtcta
      841 ccgcctcgtc gacttcgatg aactgacgct cgcagagctc ggcgcgcacc ccgatcgcct
      901 tcgcgccgta tcgcaaaatt tcccccgcct ttcgatgcga aaagtgcggc atttgcttgg
      961 gtcgatgcga gcccacgccg gtcgcgacgc ggtcctcaac ctcgcgaacc cagcgctccc
     1021 gcttgtccca atcagcgatc gcttccgcac cgaggcccgg cacgcgcatg tagagaacgc
     1081 cgcaggaaat gtcgtagcca gagcccgcct ggatgatcgt atcttcggtc acgatgacgc
     1141 agccgaccgg cacgccgtag ccggagtgtg catcgggcat cagatacgcg ccgatcacac
     1201 cttcgtagct cgccgcggac gcgagctgct gccacatcga ctcttcgctc gcctcgtaca
     1261 gctgctcgga gaagaaggcg tgcgcgtcga cgcgcatgga ccccgtcttc ggcagcacgt
     1321 agtggttcgg gtcgatgggt cgggcgaaat acttcaagga catggtcgtt ctcgctcgta
     1381 gagcttaatc agcaaaagtg acccgacgag gtttggttcc gtcgaccgac tcgcgtcgat
     1441 tgcggccggc gcattttcac cttctgcgcg aaggtgccgc tcgccccgcg agcattcgtt
     1501 tccgtacgct cgccatcact agcgcgcccg cggctctcgc ctcacggacg cggttcgcta
     1561 ccttagggcc gttattgtag acggacccgg cattcgggtc ggtgctgtcg cgtaacaaga
     1621 ccaacgacgg caagggaagc ggagacgttt caaatgctct gccactgagc taccggcgtg
     1681 agccgggcga gattcgaact cgcgaccgtt tgattgggat gtagtccccg cagcatccgt
     1741 catcggagct tcggccacgc caggttgacg aggcgtgggt tgatagaggc cgacaagtat
     1801 gcgaacgaca gcacagtttc agaatgtagt cgttcgcgca tccggcccac agaacgatgc
     1861 gtgctcgagc tcggtgagcg gagcacgcta gagatggcgc cggcaagagt gcagagacat
     1921 cgttcccaca gcccttgcga gctctggaga accctgcttg taggtgtagt ccctgcggca
     1981 tccggcgttt gatcgattcg actagagcat ggcggcttct ttcgttgaga ataggggccg
     2041 gcaagagtgc agagacatcg ttctcggagt cctttcgaac tccggagaac cacttaacag
     2101 gtggtgtagt ccctgcggca tccggcccgt gcatttcaga ttgagccgcg gcgcggaccg
     2161 ctttcgagac gatccgcgcc cctgcatcag acgcgaaact cgttcgcatc attcacgcca
     2221 gcgcgacctt ttcgatctcg ccgacccaat gctcggcgtc gagttcgccc ttcgcaaaga
     2281 gcgaaacgac ttcgaaaacg gagtcggaga agccgccgac gttgagcacg tccgcgcgct
     2341 cgctcacctg agtcgagcca tatggctgga cgtcgatgag cacgagcttc gcgttcggac
     2401 tgcgcttgcg gaagcgctcc cattcctcgg ccattggcgt cgcgcgctgc gaaacgccga
     2461 aatctgccca agactggttg tcggagacgt agatgacgag atcgccctcg gccttgtgct
     2521 tgttcaagtg tcggagcggc gcgctgcaag ccgttccgcc gctgggcaag ctggcgagga
     2581 acgcggcgtt cgtcatcacc gagtcgagcg ggttcaaccg ccgcgacatc gagacgacgt
     2641 cgtcgctgaa cgggattact tccgccgttt ggttccggcg caggaacgcc gccgcgacga
     2701 ggcccgccac gtcgacgcac cgaaccttcg agctgcttcc cttgcggtgc cccgtcacgg
     2761 gcgactgcat cgaaccggac acgtccggac agacgtagac cttacccggg accgcgggca
     2821 cgttctcgat cgagacttcc aaggccaact gcagcgcgag cgcgagctcg ttcggcagag
     2881 tagcctgggc cgccgtgtaa gccgcgagca gctggtacgg aaagacgcga gccttgcgga
     2941 ccgcgtccgg atccgagagc cgcgccgcgg cgaagtcgat catgtcgcgg tgttcgaaaa
     3001 caccgtgacg gagcagcgtg ttcaggttca tgcgaagttg cgtgaaggac atgcgccgcg
     3061 cgatctgcat ccacatttcg cgcgagagcg gcaacgccgt gagcatctcg aacggcacgt
     3121 ccggcacttc gggcgagcca ctcttgaaag cttcgaaggt ctgcacgacc gccggcaggc
     3181 gctcgaacgc gacgtcctta ccgatcaagt aaccgtaaag agcctcacgt tcggcgtccg
     3241 cgacgccgtt caccaccggc gaaggacgaa gcatcttgat gacgtccttc atcgatggat
     3301 cattgccgat cgactgacgg aacaacgccg cgggcgtgcg cttgtcgaac caggtccgca
     3361 cgagccgctt cggcgccgta ccgaacgact tccggccgac cacgcccgag cgcacgattt
     3421 gcacgaagtt ccgcagcatc tttccgttgt cgatcacccg atcgaacacc agcgaaagca
     3481 ggcgcacgtc gaccgcggcg aggtacgcca acaagaacgc cggcatatcc ttcatgcgcg
     3541 cgcgttcccg tgcgtatacg gccgtcttgg cgacgaagac agggtcgacg gcgcgacaga
     3601 gctcgagcac tcgttcgagc tgctcgcctt cggtcgcata aaaggtacgg ttgaacgtac
     3661 cggtcgcggc gtactgcgcg agcgcgtgcg aaggagtgaa ctcgtaagca gttccgcctg
     3721 cttcgttctt gcccttggcg gtgagcgcct tggtcgcgga agcaaaaaga gtcttcaatg
     3781 ccatgttcat tctccggttt catgcgcgcc gatgcgcgcc aagatgcatg cgcgccgatg
     3841 cgcgccaaga tgcgtgcgcg ccgatgcgcg ccaagatgtg cggcgacgag agtgcgaaga
     3901 ggtcattgcc cccaaagtgg gcacgcctcc aaagtccgag tgcgctaacg gcacgagtgc
     3961 cgattagcgc cttgcggagg cgtctggaat cgaaccgatg tactcctcac ggcattcgcc
     4021 gcgtaaacca aactcgtatt cgagcgctcg cactcacggc gagcccgatc cacggccccg
     4081 gcacgacacg ccgcgaccca ctgagtttcc ctgcgagcaa cgaggaactc agactgttcg
     4141 accccgaaaa cgccgaaggc cgccttttct tacggggcgg ccttcgaaag cttcatcaac
     4201 ggttgaagct cagatccgcc tgtgatcggg atggccgtaa ttcagcgcgc acaacagccc
     4261 gcgctgggat gattttcggg gctgaatggc ggtgagcatg cggattccca cttcgtagat
     4321 gcacgggtgc gtcgtcccgt tgagcgagtt tatgaaggag cgcgctcgat tcgtcaagcg
     4381 cgaactcaga ctttgtgctt gcgcaaatcg ttgcgcaaac ctggcactga ctctgtatcg
     4441 aagacgatgc gcagtgttgg agtgagccta ttccggcttg tgttcgccgc tcgagtccgc
     4501 ggcggcgccg tgtttacgag gatctgcgga ggcttgccag agcgcgccgt cgcgcgtgag
     4561 tacttgcacg gccgtcgcaa agtccggagc ttttttggcg cgctcgccgc gatacgcgag
     4621 atcttcgaag agggagggcg gagcgctggc ttcgggccaa atggtgtcgc cttttagagg
     4681 cacgaagaag cgctgttgat gcgtcagctc ttccgcagac tttccgaaga cgagccgcgc
     4741 cacgacgagc tgcgtgacat tcgtcgaaat ggccatcccg ccagaaccgc cgatcgcgag
     4801 cacggggcga ccgtctttca gcgcgatggt cggcgtcatg ctcgacaccg ggcgcgcccc
     4861 cgcacgcggc gcgttcggcg aagcggcgat cccgagcgcc ttcgcgtcgc gcgcgagcgt
     4921 gaagtcgtcg agctcgtcgt tcaacacgat gccggagttc ttcgcgacga gcttcgctcc
     4981 gaacaagcga tttactgtcg tcgtgagcga aaccaccatc cccgtagagt cagcgacgac
     5041 gatgtggtga gtaccgtgct cctccgaaac cagcagaggg aggctgtggg ttcgatcgag
     5101 tgacagcgcg cgccgcttcg ccgccaagcg ctcgggagcg aggagctttg cggtgtcgac
     5161 tggcgtaaag tcggggtcgc tcgcgtaacg gaagcgatcg gcgaatgcgc tccgcgccgc
     5221 ttcggccagc aggtgctgat aggcgccact ctggtaccca aggcggccga gctcactggc
     5281 tgagagcatg cccaaggtct ccgccaagag gagcccgccc gccgaaggcg gcggcatcgt
     5341 atacacctcg tgcccggcga agctcacgtg cagcgctttg cgctctttga cggcgtagtc
     5401 gtcgaggtcg ctctgcgcca acgcgctgcc cgcggagcgc gcggtctcga cgagctcggt
     5461 cgcgatggct ccttggtaaa ttgccttcgg cccctccgcg gcgacgcgag ccagcgtgcg
     5521 cgacaacggc aggttcttga ccacacggcc ttcgagcgcg gcgcggcctt gtggaaaaaa
     5581 gagctgagcg agcgacttgt cgcgcgcgag ctccgcattc ccagcgagcg agcgggccag
     5641 gtgtgggccc accgggaaac cctggccggc gaggcgcacc gcgggcgctg ccagcgtcgc
     5701 ccaagcgcgc ttcccaaagc gacgcgagag ctcgaacaag cccgcagact ctcccggtac
     5761 gccgatcaaa tgcccgcgct cttcgtacgg caagggccgg cgctcgagcg cttcgacgga
     5821 gacgccgcgc ggtgcggtct cgcgaaaatc gaggatgctc ggctcggcgt cgtttgggcg
     5881 cagcaccatc gcgaagccgc cgcccccgag accgctcgag gtcggcgaga ccacgcccgc
     5941 ggcaaacgcc gctgcgatcg ccgcgtcgac ggcgttgcct ccctgcttca agacctcgag
     6001 ggccgcgcgg gtggcgagcg ggtgctcact cgcaaccgcg cctcgctcgg cgagcggcgc
     6061 cccctgcaaa cgcggcgaaa gcgcgacgag ccccgccgca gcagccaaag gaccgaacca
     6121 ggctggaagc atcgcgagta aacgcacgcc ttgtcttaca cccaagaaga cgggacgacg
     6181 gaaaaatcgg acgccgcagc ccgagcccaa ccccagcgcg gctgatatgc tgcgcgcgtg
     6241 cgcctcctat tcgtgatgga tccgttcaac ggtctgacct tcgggaaaga caccagtttc
     6301 gctttcatgc gcggcgccgc ccgtcgcggg cacgaggtct ggcattgctt gccggaagac
     6361 gtgaccagcc gagggcgcga cgtgttcgcg cgagcgacgc gcttgtcggt caatcaagcg
     6421 gc
//
LOCUS       DASTKS010000013         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1116103, whole genome shotgun sequence.
ACCESSION   DASTKS010000013 DASTKS010000000
VERSION     DASTKS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116103"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..316
                     /locus_tag="VFQ35_00225"
     CDS             98..316
                     /locus_tag="VFQ35_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HET9929075.1"
                     /translation="MTADSDDARVYTVLVNQEEQYSIWPADSEIPAGWSEVGKRGNKE
                     ECLAHIGRVWTDMRPLSLRKWLAERDAG"
     gene            521..1552
                     /locus_tag="VFQ35_00230"
     CDS             521..1552
                     /locus_tag="VFQ35_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929076.1"
                     /translation="MFLSPVTHAADATPAATGTSAEQPAPATPSPAQPANVKEAAARF
                     ERGLALYDDGDYEAALVEFSRAYELQPTYKILYNIAKIERVKNDYSTALNHYEHYLEQ
                     GGAEIPAERREEVEKEIAVLKERVALVTINANVEGAVVYIDGIPLCSARTLDPTCVGT
                     TPLRGPVIVNPGKRKITAVKQGYQNADVQLTLAGGDKNSVKLDLFDLRPKSVDTAPRD
                     RAIISWGVTGALAVGAGVMGVLTLSKKSDYDHDRDLPPCANQTVNSNSCLTDTARTLS
                     DDKDTTKTFALVTDVLAGAAVVGAVVSTYFTIKASKAGDAEQHAERFRIRNLRVGAGP
                     GSAVLMGSF"
     gene            complement(1620..>3109)
                     /locus_tag="VFQ35_00235"
     CDS             complement(1620..>3109)
                     /locus_tag="VFQ35_00235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929077.1"
                     /translation="RFVREGYVANRVEHDGAVAVLDDDITDDGSAFLVMELLHGVTLE
                     RLWEANGEHLPLECVLAIAEQLLDVLAAAHAQSIVHRDLKPANVFVTRHGVVKILDFG
                     IARLRDGDGNKTETGTTLGTPLFMPPEQAAGRSRDVDARTDLWSVGATMFSLLTGQYV
                     HDGENAAQVLIASATTKARSLATVMPALPPEIIEIVDRALAFHNEDRWESASAMRTAI
                     MNAQEILGVPKSRDFLGNIVRSTAPGLGEAQSTPVTTPAGTDRTERIPFTPVHGSPLD
                     ALPPPTPDKVAAQDLDITKRSTQAPAEKRPAGASQIELTESSARAVTSPTENPAARSR
                     SRSVAGGFLIAATAAVIGGVFAVRFVAGPSNAQAPSASAPAVVASPPAAAINQMAQPA
                     KPAEPAAATTEQGASSPAVVPVASAPPASAANVVPAPAANPKWGHKIVRGARTTPAPA
                     AASAPASATPVAAAPSPQPAAPPAPAPAPATTPVKKPVLDRTSPF"
BASE COUNT          543 a          969 c         1056 g          541 t
ORIGIN      
        1 gagttcggtt gcgttttcac gcgcgacaga atcacgagtt tgcggccggt tcgctgaggt
       61 cgcggccgaa cccgctcaaa tgctaaggtc gacggtgatg accgctgata gcgatgacgc
      121 gcgggtctac accgtcttag tcaatcagga agagcagtac tcgatttggc ccgctgactc
      181 ggaaattcct gctggttgga gcgaggtagg aaagcgcgga aacaaagagg agtgcttggc
      241 gcacatcggt cgagtttgga cggacatgcg accgctcagc ctccgaaaat ggttggccga
      301 gcgggatgca ggttgaaggc aacgcgtctc ccgcttcggc gtggtttcat tttcggcaaa
      361 gtcattcttg ttgctctaga gcttgcgacg aagctagtct ccacgatgct tcgcctcttg
      421 gtttggagcc gaagcaaggc agcctcccat gaacgcttct gaagttctca cgagacgcgc
      481 aggccggaac gttggaatcg cagcgttgtc ggcagcggcg atgttcctct cgcccgtcac
      541 tcatgccgct gacgccacgc ccgccgcgac ggggaccagc gcggagcagc ctgcgcccgc
      601 cacgccgagc ccagcccaac ccgccaacgt gaaagaggcg gcagctcgtt tcgagcgcgg
      661 cttggcgctg tacgatgacg gcgactacga agcggctctg gtggagttca gccgcgcgta
      721 cgagctccaa ccgacttaca agattctcta caacatcgcc aagatcgagc gcgtgaagaa
      781 cgactactcg accgcgttga accactacga gcactacctc gagcagggcg gcgcggagat
      841 cccggccgag cgccgcgagg aagtggagaa ggagatcgcg gtcttgaagg agcgcgtcgc
      901 cctcgtgacg atcaacgcga acgtcgaagg cgcggtggtc tacatcgacg gtattccgct
      961 ctgcagtgcg cggacgttgg acccgacgtg cgtgggcacg acaccgctgc gtggcccggt
     1021 catcgtgaac cccggcaagc gaaagatcac cgccgtaaag cagggctacc agaacgccga
     1081 cgtgcagctg acgctcgcgg gcggcgacaa gaacagcgtc aagctcgacc tattcgacct
     1141 tcgcccgaag agcgtggata cggcaccgcg tgaccgcgcc atcatctctt ggggcgtgac
     1201 cggcgcgctc gccgtcggtg cgggcgtgat gggcgtcctc actctctcga agaagagcga
     1261 ctacgatcac gatcgcgacc ttcccccgtg cgcgaaccag accgtgaact ccaactcctg
     1321 cctcacggac acggcgcgaa cgctgtcgga cgacaaagac accaccaaga ccttcgcgct
     1381 ggtcaccgac gtgcttgctg gtgccgccgt cgttggcgcc gtcgtttcca cgtactttac
     1441 gatcaaggct tccaaagccg gtgacgccga gcagcacgcc gagcggttcc ggataaggaa
     1501 cctgcgcgtc ggcgcgggac cgggcagtgc cgtcctgatg ggctcgttct agctcgtttt
     1561 gactgcgagt cgtcggcggg cacggctccg tgaagccgcc gtgcgttacc cggagcgctt
     1621 caaaacgggc tggtgcggtc gagcacgggc ttcttgacgg gagtcgtagc cggcgctggt
     1681 gcgggagcag gcggggccgc cggttgaggt gacggtgcgg ccgcgaccgg agtggccgac
     1741 gcaggcgcag aagcggccgc gggcgccggt gtcgtgcgcg cgccgcggac gatcttgtga
     1801 ccccatttcg gatttgctgc cggggcgggc accacgttcg cagccgatgc tggcggcgca
     1861 ctcgctaccg gcacgacggc tggactgctc gctccttgtt ccgtggtcgc cgccgcaggt
     1921 tcggcaggct ttgcgggctg cgccatttga ttgatcgcgg ccgcgggtgg gctagccacg
     1981 accgctggcg cagaggcgct cggggcctgc gcattcgacg gcccggcgac gaaacgcacg
     2041 gcgaaaacgc caccgatcac cgcggccgtg gccgcgatga gaaagccgcc cgcgactgat
     2101 cgagagcgag agcgagcggc aggattttcg gttggcgacg tgacggcccg cgcgctcgac
     2161 tcggtgagct ctatctgcga agcgcctgcg ggacgctttt cggcgggagc ctgagtgctg
     2221 cgcttggtga tatccaagtc ttgcgccgcg accttgtctg gcgtcggagg tggcaaggcg
     2281 tcgagcggcg agccgtgcac tggcgtgaac gggatgcgct cggtgcggtc ggtaccggcc
     2341 ggggtagtga cgggcgtgga ttgcgcctcg ccgaggcccg gcgccgtcga ccgcacgatg
     2401 ttgccgagga agtcacgtga cttcggaacg cccagtattt cctgcgcgtt cataattgcc
     2461 gtgcgcatcg cgctcgccga ttcccaacgg tcctcgttgt ggaaggcgag cgctcgatcg
     2521 acgatctcga tgatctcggg agggagcgct ggcatcacgg ttgccaacga gcgcgctttc
     2581 gtcgttgcgg aggcgatcag cacttgagcg gcgttctcac cgtcgtgtac gtactgaccg
     2641 gtgaggaggc tgaacatcgt tgcgccgact gaccagagat cagtccgggc gtcgacgtcg
     2701 cgcgacctgc cagccgcttg ctccggcggc atgaacaagg gcgtgccgag ggtggtgccc
     2761 gtctcggtct tgttgccgtc accgtcgcgc agccgcgcga tgccaaagtc caaaattttg
     2821 acgactccgt gccgtgttac gaacacgttg gcgggcttca ggtcgcgatg gacgatgctt
     2881 tgcgcatgag cggccgccaa aacgtcgagg agctgctcgg cgatggcgag gacgcattcg
     2941 agcggcaagt gctcgccgtt cgcctcccaa agccgctcga gcgtcacgcc gtgcaagagc
     3001 tccatgacga gaaacgccga gccgtcgtcg gtgatgtcgt cgtcgagcac cgcgaccgcg
     3061 ccatcgtgct cgacgcgatt cgcaacgtac ccctcgcgca cgaaacgtt
//
LOCUS       DASTKS010000014        13245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1562558, whole genome shotgun sequence.
ACCESSION   DASTKS010000014 DASTKS010000000
VERSION     DASTKS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562558"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFQ35_00240"
     CDS             complement(<1..242)
                     /locus_tag="VFQ35_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9929078.1"
                     /translation="MKILVPIKRVADPDNANKIKIASAGNAVETSGLEWKMNPFDEYA
                     LETALRLTENGKAPKTRLGEVVVVTFAPKDAETTLRA"
     gene            392..1999
                     /gene="nadB"
                     /locus_tag="VFQ35_00245"
     CDS             392..1999
                     /gene="nadB"
                     /locus_tag="VFQ35_00245"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET9929079.1"
                     /translation="MALKTDYLVLGSGIAGLTFALHAAAHGEVLLVTKRAAHDTATNY
                     AQGGVAAVLSPDDSFDKHAEDTKVAGAGLCHEVVVDICVREAPAAIHWLTSIGARFSE
                     GDPGKLDLGREGGHTERRIVHAGDITGNEIQRALWEAVRESPNIRVLEWHMGVDLITL
                     SAFGGPDRCVGAYVLDERRGVVETVLARATLLATGGAGKVYLYTSNPDVATGDGIAMA
                     YRAGAEIANMEFYQFHPTVLYEPKATSFLISEALRGEGAVLRLPDGEAFMTRHHPMGD
                     LAPRDIVARAIDYEMKRTGSEHVLLDITQKPADFVRSRFPNIHAYCLRFGIDMTTDPL
                     PVVPAAHYMCGGVTSDLHGRTTLPGLWVIGETACTGLHGANRLASNSLLEGLVFGRRA
                     AKRLETQIDELRSEGFPRVPDWQTGSAVPSHEAVVVTHNWDELRRTMWNYVGIVRSDA
                     RLRRAARRVALLEQEIREYYWRHLVTRDLLELRNIATVAELIINSALSRRESRGLHTT
                     LDYAETSPAFEADTVVKRGVLPHLRGR"
     gene            2006..2794
                     /locus_tag="VFQ35_00250"
     CDS             2006..2794
                     /locus_tag="VFQ35_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9929080.1"
                     /translation="MRDRLGVVLASSFAVLLTLASVIALGLGVSLASSWWPAGRGHLV
                     LAAAVEVVVYLAFSRALAGNLRFTALCPFTRPSWQTAVSAVLLGVFLHGPLDFVEAWV
                     QRWAPLPEHVLRERALGLQPEFALERVLLFVLVAGAVPVVEEVFFRGALFARLAPVLG
                     AFGTLLVTSVCFTASHGEPRSWPPLLAVAFVLGVLRQWYGSLWPSVLLHATFNATTLT
                     VVFLSPRGSFERPEPSFLAASIGTLVSAALFHRLWRSPAGARLA"
     gene            2791..3888
                     /locus_tag="VFQ35_00255"
     CDS             2791..3888
                     /locus_tag="VFQ35_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9929081.1"
                     /translation="MTARIALCLPGGGAMGGLYQIGALAAIEDAVEDFSANSFDMYVG
                     SSSGASLASVLAAGVPVQRLYRAFLDPADVYFGLERKHLLRVDLGEWRRTLITALKAM
                     RHAAGGVLSRDSSSALPSALWHELDRLYDSLPAGLFTLEGYEHFLEDFFVRRSVPNTF
                     SAMPRPLRILAHDLDTGAPACFGAPGLGNVPVTRACIASMAIPPFFSPVRIGDRHYVN
                     PGAGQISHLDVAVEAGAEVLFVIHSMVPVNVETVPTGHGHRDSLRDKGMMWVLNQAIR
                     IGMQRLIDEASQRVASSGSVAVLRIEPVPQDGILFMYNPTSFDARRRMLEHAYRSTRL
                     ELAQRFANADPTLARAGLRPRMAARSIPPSE"
     gene            3892..4389
                     /locus_tag="VFQ35_00260"
     CDS             3892..4389
                     /locus_tag="VFQ35_00260"
                     /inference="COORDINATES: protein motif:HMM:NF023145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tudor-knot domain-containing protein"
                     /protein_id="HET9929082.1"
                     /translation="MRTRWFDAALVVMALGLGACKKPYRVGEHVLVRWEDGSPQLYPA
                     YVLERVSQTRYKVHFEGYDARFDEDVGVDRIEGRVEGPVPTPPPPRKVARAVGSSNPT
                     DAGAALLVNPYKPSDRVRVRWRGSIYTATVLEVVSKDRVRIHYEGFENAWDEIVLIDR
                     IVARR"
     gene            complement(4395..4742)
                     /locus_tag="VFQ35_00265"
     CDS             complement(4395..4742)
                     /locus_tag="VFQ35_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929083.1"
                     /translation="MIQLRTGLAASALVLLGCASRLGSAMDAFEAGRLPEAAAEFRSL
                     EPQFPRLGERDQARYALFFGLTELALGDLDGAERWLLPLKVAVEHDPLRLSDAERGAL
                     FAALRSTGRMPGE"
     gene            4842..5396
                     /locus_tag="VFQ35_00270"
     CDS             4842..5396
                     /locus_tag="VFQ35_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET9929084.1"
                     /translation="MLDDLSARLAENLRRLREARGLTQQDLSAASGVPRPTLAHLESG
                     SANPTLSVIARVAHALSVPMEELVAQANANIEFRPSKSLPRHERSGARVVEVCPEPPS
                     GIVVERIELSHRAELVREGSRQADRIYVVCERGELDVGSGEEHCALKAGDLAMIRRGA
                     PHVITQRGRSAAVAYSVRIGAWSG"
     gene            5524..7338
                     /gene="typA"
                     /locus_tag="VFQ35_00275"
     CDS             5524..7338
                     /gene="typA"
                     /locus_tag="VFQ35_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET9929085.1"
                     /translation="MTSQRNDIRNIAIIAHVDHGKTTLVDAMLRQTGVFRENEAVVER
                     VMDSGDLERERGITILAKHCSVRFRDTLINIIDTPGHSDFGGEVERTLKMADGCLLLV
                     DAAEGPLPQTRFVLSKAIELELPIIVVINKIDRSDARPDEVLSEAFDLFCELEASDAI
                     ADFPVIYAIGKEGMAKRKLEEEGKGLTPLLETILERIPAPKVDLDGPLQFGVHNIEHD
                     EYVGRLAIGRVRRGKISVGQNVTCLGLNGNVTQRVSAIYMFEGTRRIKVERASAGEIV
                     ALAGFEDVQIGDTIAAVETPEPLPRIEVEEPTIKIRFVVNSSPFAGKVGKWVTSRHLR
                     DRLFREARRNLALRVEETEEPDTFVVLGRGELMLAILAETMRREGYEFALGMPEVIVR
                     EVDGVKSEPVERVVIDVPEQFIGAITQRLGERRGQMTKMTNLGFGRARMEFRVPSRGL
                     IGFRSAFLTETRGMGLLNSMFDGWEPYAGPMLRRPNGALVADRAGETTPYALFHLQPR
                     GVLFVEPGTKVYEGMIIGEHNRPNDLDVNATREKKLTNIRAAGRDENVILTPPHTLNI
                     ELALEFIDRDELAEITPDAVRIRKRILDTSRRPRRDDE"
     gene            complement(7370..9046)
                     /gene="hflX"
                     /locus_tag="VFQ35_00280"
     CDS             complement(7370..9046)
                     /gene="hflX"
                     /locus_tag="VFQ35_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9929086.1"
                     /translation="MTEIFGNTTGLPPSSLKTLERIYRRRVPNDRIVTHELVRSLVDA
                     SNETKRQVGALVHRSGEVDCVIVGHATGLLLPDIGRLRAAEGRFRALRLVHTHLFGEP
                     LTRDDLIDLTRLRLDLVAAIQLTPEGEPRALTWAYNVPSRDANARPYEIVPPQPYGKE
                     QPNFGELIASLEAEFGELRRARVVRGKDGRAILVHVADKRRAGALNRAKAELAELLSL
                     SETAGVDVADSVIQVREHPDPRFVVGRGKLDEIVMRAIDLDAETLIFDCELSPSQASS
                     LAKQTDLKIIDRTQLILDIFAQRAESRDGKLQVELAQLKYALPRLQQKDDSLSRLTGG
                     IGGRGPGETKLEIGRRRARERVHRLEKELESLEAQRRERRKRRREGDLPVVALVGYTN
                     AGKSTLLNTLTQAGVLAEDKLFATLDPRARRLMLPNGRSVVLTDTVGFIRRMPKDLFA
                     AFRATFEEATDADVIVEVVDVSDPEHEEHVQTTTEVLAELGLELTPRVRVYNKIDRLE
                     PSERDAFEHQAGSVCVSALEKPSVVPLLNALERLLPAPAAEHDALAVAAV"
     gene            9225..10187
                     /locus_tag="VFQ35_00285"
     CDS             9225..10187
                     /locus_tag="VFQ35_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET9929087.1"
                     /translation="MATLTPLSLADAQGLAARFGLDLAALEPLAAGSVNSNFALEMRD
                     GTRYFARLYEEQPLAGAQVEVALLATLARRGARVVAPCETAQGERVLLHAGKPFAVFP
                     WVDGDWLCLRRVNAAHCEAVGAELARVHAASSEVPPLSEGRFRPRDMLARLERVEREG
                     PPRLAPALAEIRARYAEYLPKRDPELPRGICHGDLFRDNVLWQGPRISALLDFESVYS
                     GSFAYDLMVTALAWCYTDSVVVPHVEAMFRGYASVRPLSAAEREALPVEGALACLRFA
                     TTRITDFELRAAPGQAPGRDYKRFLERLRALQSGAFDSAFQSLG"
     gene            10225..11604
                     /gene="argH"
                     /locus_tag="VFQ35_00290"
     CDS             10225..11604
                     /gene="argH"
                     /locus_tag="VFQ35_00290"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET9929088.1"
                     /translation="MTVARGGRLPQSIDAAMERLNTSVDIDSELWAEDIQGSIAHARG
                     LARAGVLSASEAETIVGGLESIAAEIRNGTFVWDREKEDVHMNIESRLTALVGSVGGK
                     LHTGRSRNDQVATDLRLYCRRRGQELLAAISDLGSAILERARGEIDTLMPAYTHLQRA
                     QPSRLSHHLMAWQEFLLRDRGRLRDALTRANESPLGAGAVAGTGFSLDRAGVARELGF
                     ERAMRNSIDATGSRDFLMEIASALGILGVHLSRIGEEIVLWSTVEFGFMTLSDAFSTS
                     SSMMPQKKNPDVAELVRGKAARLVGSLTSLLVLEKSLAFGYGRDLQEDKRPIFDAFEA
                     AEVSVRALTGAILTATFQRETMQRALGRGHLCATDLADFLVQRGLPFREAHHVVGGLV
                     REAESRGVQLGELPGDVLEGAHAALKGPELASALDPAAAVERRSLVGGPARARVLAAI
                     SEAERDWSA"
     gene            complement(11616..12641)
                     /locus_tag="VFQ35_00295"
     CDS             complement(11616..12641)
                     /locus_tag="VFQ35_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929089.1"
                     /translation="MNPTLPLWILSAATLTEPMSVPDSFANLPSLSRFSARWSVNFRE
                     VPHEIDTLGARADAEQAESVRRIEALFVAAKTAIGALRAAEADGALAEAEQLIRSHPE
                     LPQGAWLLAELHTTRAERVEGDDPILARELARRAAILEGERGQAFRDATRAGTPLALP
                     EARELAVTGPDSRARIEWDGEASALNVASRSGEHHVRVLRGERLVWAGWVTVGDTATT
                     VNLPVPAVTPCSADDLDGAVEAEPSPRAHPRTQCSDWAAARVRNGELELARCHAGSCG
                     AWEHASAPPPTRNVSNEAKSPLPRWLTYAVVGAGTALVAGIVLASSGAFSSSDPTRER
                     WVYSGFK"
     gene            12729..>13245
                     /locus_tag="VFQ35_00300"
     CDS             12729..>13245
                     /locus_tag="VFQ35_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HET9929090.1"
                     /translation="MQLDFVNRELTIKLVYYGPALSGKTTNLQALHNVAAPGSSGRLM
                     TLETRDDRTLFFDLLPLTFKSQGSLAVRLKVFTVPGQVIHASTRRLVVQGADGIAFIA
                     DSRVSETQHNAEAFLDLKQNLKEHGLALRKMPLIIQFNKRDMPAVRSDEEIAQLAAQG
                     REPVYMAIATRG"
BASE COUNT         2089 a         4365 c         4478 g         2313 t
ORIGIN      
        1 gcgcgcagcg tcgtctccgc gtccttcggc gcgaacgtga ccaccacgac ctcacccaga
       61 cgagtcttgg gcgccttgcc gttctccgtc agccggagcg cggtttcgag cgcgtactcg
      121 tcgaaggggt tcatcttcca ctcgagcccg ctggtttcga cggcgttacc agcgctcgca
      181 attttgattt tgttggcgtt gtcggggtcg gcgacgcgct tgatagggac gaggatcttc
      241 accgcgtttc tccttccacg gggctgtttc tttcattctg accgccaaag tcgcgtcgag
      301 taaagcgggc ctccccgaaa tcgctggccg aacggccgac accggagggc gcgccaatgg
      361 gcgccactgg gtggtatgag aaccgccgac gatggctctg aagaccgact acttggtgct
      421 tggcagcggg atcgctgggc tcacattcgc acttcacgcg gcggcgcacg gtgaagtgtt
      481 gctggttacg aagcgagcgg cccacgatac cgcaacgaac tacgcgcaag gtggcgtcgc
      541 ggcggtgctc tcgccggacg actcgttcga caagcacgcg gaagacacga aggtcgccgg
      601 ggcggggctt tgccacgagg tggtcgtcga tatctgcgtg cgcgaggcgc cagccgcgat
      661 tcactggctc accagcatcg gcgcgcgttt ctccgagggc gatccgggca agctcgactt
      721 gggtagggaa ggcggccaca ccgagcggcg catcgttcac gcgggcgaca tcaccggcaa
      781 cgagattcag cgcgcgctgt gggaagccgt tcgcgagtcg ccgaacatcc gcgtgctcga
      841 gtggcacatg ggcgtcgatc tcatcacgct ctcggccttc ggcgggcccg atcgctgcgt
      901 cggcgcgtac gtgctcgacg agcggcgcgg cgtcgtcgag acggtgcttg cgcgcgcgac
      961 gctgctcgcg acgggcggcg ccgggaaagt ctacctctac acgtcgaatc ccgatgtcgc
     1021 gaccggcgac ggtatcgcca tggcgtatcg agccggcgcc gagatcgcga acatggaatt
     1081 ttaccaattc catcccacgg tgctctacga gcccaaggcc acgagctttc tcatcagcga
     1141 agcgctgcgc ggcgaaggcg ccgtgcttcg cttgcccgac ggcgaagcgt tcatgacgag
     1201 acatcacccg atgggtgatc tcgcgccgcg cgatatcgtc gctcgcgcga tcgactacga
     1261 gatgaagcgc actggctccg agcacgtgct gctcgacatc acgcaaaagc ccgctgattt
     1321 cgtgcggagc cgcttcccca acatccacgc ctactgtctg cgcttcggca tcgacatgac
     1381 cacggatccg ctgccggtgg tgcccgcggc gcactacatg tgcggcggcg tcacctctga
     1441 tttgcacggg cgcacgacgc tgcctgggct ctgggtgatc ggggagacgg cgtgtacggg
     1501 cctgcacggc gcgaaccgtc tcgcatccaa ctctctcctc gagggtctcg tgttcggtcg
     1561 ccgcgcggca aagcgcctcg agacacaaat cgacgagctg cgctccgaag ggtttccgcg
     1621 cgtgccggac tggcaaacgg ggtcggccgt gccgagccac gaagcggtcg tcgtgacgca
     1681 caactgggac gagctccgcc gcaccatgtg gaactacgtc ggcatcgtcc gcagcgacgc
     1741 gcgcttgcgc cgcgccgcgc gccgcgtcgc gctcctggag caggagattc gcgagtacta
     1801 ctggcgccac ctcgtcactc gcgacttgct cgagctcagg aatatcgcga ccgtcgccga
     1861 gctcatcatc aactccgcct tgagtcgtag agagagccgg ggcctccaca cgacgctcga
     1921 ttacgcagag acttcgcccg ctttcgaggc cgacacggtg gtaaagcgcg gggtattgcc
     1981 gcacctccga ggtcgctgaa gccgaatgcg cgatcggctc ggggtggtcc tcgcgtcgtc
     2041 gttcgccgtg ctgctcacgc tggcgtcggt gatcgcgctc gggctgggcg tctctttggc
     2101 ctcgtcttgg tggccagcgg ggcgaggaca cctcgtgctc gccgcggcgg tcgaggtcgt
     2161 cgtttatctc gcgttcagcc gtgcactcgc ggggaacttg cggttcacgg cgctatgccc
     2221 attcacgcgg ccgagctggc agacggcggt gagcgcggtg ctgctcggcg tgttcttgca
     2281 cggtcctttg gacttcgtgg aagcgtgggt gcagcgctgg gcgccgttac cagagcacgt
     2341 cctgcgggag cgcgcgctcg gactgcaacc cgagttcgct ctcgagcgag ttctgctgtt
     2401 cgtgctcgta gcgggcgcgg tgcccgtggt cgaagaggta ttcttccgcg gggcgctgtt
     2461 tgcgcggctc gcgccggtgc tcggtgcctt cgggacgttg ctcgtcacat cggtttgctt
     2521 cacggcgagc cacggggagc cgcgttcatg gccgcctttg ctggcggtgg cgttcgtcct
     2581 cggtgttctt cgtcagtggt acggttcgtt gtggccgtcc gtcttgcttc acgcgacgtt
     2641 caatgcaacg acgctcaccg tggtcttttt gtcacctcgc ggcagcttcg aacggcctga
     2701 gccgtcgttt ttggctgctt ccatcggtac tctggtgagc gcggcgctgt tccatcggct
     2761 gtggcgttcc ccggccggcg cgaggctcgc atgacggcgc gcatcgcgct gtgcctgccg
     2821 ggcggtgggg cgatgggggg cctttaccaa atcggcgcgc tcgccgcgat cgaggacgcg
     2881 gtggaagact tcagcgccaa ctcgttcgac atgtacgtgg gcagctcgag cggcgcgagc
     2941 ctcgcgtcgg tgctggcggc gggcgtgccc gttcagcgct tgtaccgcgc gttcctcgat
     3001 cccgccgacg tctacttcgg tctcgagcgc aaacacctct tgcgggtcga cctcggggag
     3061 tggcggcgca cgctgatcac cgcgctcaag gcgatgcgcc acgcagctgg gggggtgctg
     3121 tcgcgtgact cgagcagcgc cttaccttcg gcgctgtggc acgagctcga tcgcctatac
     3181 gactcgctcc ccgcgggcct gttcacgctg gaggggtacg agcacttcct cgaagacttt
     3241 ttcgtgcggc gcagcgtgcc aaacacattt tcggcgatgc cgcggccttt acggatcttg
     3301 gcccacgacc tcgacaccgg cgcgccggct tgcttcggcg cgcctggtct cggcaacgtg
     3361 ccggtgacgc gcgcttgcat cgcgtcgatg gcgatcccgc cgtttttctc gcccgttcgc
     3421 atcggcgacc gccattacgt aaaccccggc gcggggcaga tctcccacct cgacgtagcg
     3481 gtggaagcag gcgccgaggt gctgttcgtg attcactcga tggtcccggt gaacgtcgag
     3541 acggtgccga cggggcatgg ccaccgcgac agcttgcgcg acaagggcat gatgtgggtt
     3601 ttgaaccagg cgatccgcat cggcatgcag cgcttgatcg acgaggccag ccaacgcgtc
     3661 gcgagcagtg ggtccgttgc cgtgctccgc atcgagccgg tgccgcaaga tggcatcttg
     3721 ttcatgtaca acccgacgag cttcgacgcg cgacggcgca tgctcgagca cgcctatcgc
     3781 tcgactcggc tcgaactcgc gcagcgcttc gccaacgcgg acccgacgct cgcccgcgcc
     3841 gggcttcgtc cgcgaatggc cgcccgctcg attccccctt cggaatgaac gatgcgaacg
     3901 aggtggttcg acgcggcgct ggtcgtgatg gccctgggcc tcggggcttg caagaagcct
     3961 taccgagtgg gcgagcacgt gctcgtacgc tgggaggacg ggagccctca gctttacccg
     4021 gcgtacgtgc tcgaacgcgt cagccagact cgctacaagg ttcacttcga gggatacgac
     4081 gcgcgcttcg acgaggacgt cggcgtcgat cgcatcgagg ggcgagtcga gggcccggtg
     4141 ccgactccgc caccgccgcg caaggtcgcc cgcgccgtcg ggtcgtccaa cccgaccgat
     4201 gcgggcgccg cgctgctcgt caacccctac aagccgtctg atcgcgtgcg cgtccgctgg
     4261 cggggctcga tctacaccgc gaccgtcctt gaagtcgttt cgaaggaccg cgtccgcatt
     4321 cactacgaag gcttcgagaa cgcctgggac gaaatcgttc tcatcgaccg gatcgtcgcc
     4381 cgacgctgag gcttttactc ccctggcatg cggccggtgc tgcgaagcgc cgcgaagagc
     4441 gcgccgcgtt cggcgtcgct cagcctgagc gggtcgtgtt cgaccgcgac tttcagggga
     4501 agcagccagc gttcggcgcc gtccagatcg cccagcgcga gctcggtgag cccgaagaac
     4561 agtgcgtagc gcgcctgatc tcgctcgcct agccgcggaa actggggttc cagagagcga
     4621 aactcggcgg ctgcctcggg taggcggccg gcctcgaatg cgtccatggc ggacccaagc
     4681 cgcgaggcgc agcccagaag caccagcgcg ctggcggcga gacccgtgcg gagctgaatc
     4741 atgagccgat ggtcggccgc cgcggggggc gagttgcgcg gatcattttc ggtccacgag
     4801 acgtgatgaa atgagcggtc gcccggtgtt acgttatcga catgttagac gacttgtcag
     4861 cgcggctggc cgagaacttg cgacgcctcc gcgaagcgcg cgggctcacc cagcaggacc
     4921 tgagcgccgc atcgggggtg ccccggccga cccttgcgca cctcgagtcc ggctcggcca
     4981 acccgacgct ctcggtcatc gcgcgcgtcg cgcatgccct gtcggtcccg atggaagagc
     5041 tcgtggcgca agcgaacgcg aacatcgaat ttcggccgag caagagtctg cctcgccacg
     5101 agcgctcggg ggcgcgggtg gtggaggtct gtcccgagcc gccgagtggc atcgtggtcg
     5161 agcgcatcga gctttcacat cgcgcggagc tcgttcgcga ggggagccgc caggcggatc
     5221 ggatttacgt cgtttgcgag cgtggtgagc tcgacgtggg ttctggggag gaacactgcg
     5281 cgctcaaggc cggcgatctc gccatgattc ggcgcggcgc gccgcacgtc atcacccaga
     5341 gaggtcgtag cgccgcggtc gcttacagcg tgcgaatcgg cgcctggtcg ggctgaaaag
     5401 taacgatttc ggggatttcc gggggcgcac gggcgtcaat tccgtgctcg cgagaattca
     5461 agcgaaaaat cggccgcaat tccttgccgg ttgccgggac cgagccaagc ttcggcccct
     5521 cgtatgacca gccagcgcaa cgacatccgg aacatcgcca tcatcgccca cgtcgaccac
     5581 ggcaagacga ccttggtcga cgccatgctg cgtcaaaccg gtgtgttccg tgaaaacgaa
     5641 gccgtcgtcg agcgtgtgat ggactccggc gatctcgagc gcgagcgcgg tatcaccatc
     5701 ttggcgaagc actgcagcgt tcgctttcgc gacaccctga tcaacatcat cgacacgccc
     5761 ggccactccg acttcggcgg cgaggtcgag cgcacgctga agatggcgga cggctgcttg
     5821 ctcctcgtgg acgcggccga aggtccgttg ccgcagacgc gcttcgtgtt gagcaaggcg
     5881 atcgagctcg agctgccgat catcgtcgtc atcaacaaga tcgatcgctc ggacgcgcgc
     5941 cccgacgagg tgttgagcga ggcgttcgac ctcttctgcg agctcgaggc gagcgacgcc
     6001 atcgcggact tccccgtcat ctacgccatc ggcaaagagg gcatggcgaa gcgcaagctc
     6061 gaggaagagg gcaagggcct caccccgttg ctcgagacca tcctcgagcg catcccggct
     6121 cccaaggtcg atctcgacgg gccgctccag ttcggcgtcc acaacatcga gcacgacgag
     6181 tacgtggggc gcctcgccat cggtcgggtg cgccgcggca agatctcggt cggccaaaac
     6241 gtcacgtgcc ttggcctgaa cggcaacgtc acccagcgcg tgagcgcgat ttacatgttc
     6301 gaaggcacgc gccgcatcaa ggtcgagcgc gcgtcggcgg gcgagatcgt ggcgctcgcc
     6361 ggcttcgaag acgtgcagat cggcgacacg atcgccgccg tcgagacgcc ggaaccgctg
     6421 cctcgcatcg aggtcgaaga gcccacgatc aagatccgct tcgtcgtgaa cagctcgccc
     6481 ttcgccggca aagttggcaa gtgggtcacg tcgcgccacc tgcgcgaccg cctgttccgc
     6541 gaggcgcgtc gtaacctcgc cctgcgcgtc gaggagacgg aggagccgga cacgttcgtg
     6601 gttctcggac ggggtgagct gatgctagcc atcttggccg agacgatgcg ccgcgaaggc
     6661 tatgagttcg cgctcggcat gcccgaggtc atcgtgcgcg aggtcgacgg cgtgaagtcg
     6721 gagccggtcg agcgcgtcgt catcgacgtg cccgagcagt tcatcggcgc catcactcag
     6781 cgcctcggcg agcgccgcgg ccagatgacg aagatgacga acctcggctt cggccgcgcc
     6841 cgtatggagt tccgtgtccc gtcgcgcggc ctcatcggct tccgctccgc gttcctcacc
     6901 gagacgcgcg gcatgggtct tttgaactcg atgttcgacg gctgggagcc ctacgccgga
     6961 ccgatgcttc gccgcccgaa cggcgcgctc gtcgcagatc gcgccggcga gacgacgccg
     7021 tacgcgctct tccatctgca gccacgcggg gtgctgttcg tcgagccggg gaccaaggtt
     7081 tacgaaggca tgatcatcgg tgagcacaat cggccgaacg atctcgacgt gaacgcgacg
     7141 cgagagaaga agctcacgaa catccgcgcc gccggccgcg acgagaacgt gatcctcacc
     7201 ccgccccaca cgctcaacat cgagctcgcg ctcgagttca tcgaccggga cgagctcgcc
     7261 gaaatcaccc cggacgcggt gcgcattcgc aagcgcatcc tcgacacgag ccgtcgtcca
     7321 cgccgcgacg acgagtgacc tcgaggcccg tagccgtcgc cgttcgtcgt tagaccgcgg
     7381 cgacggcgag cgcgtcgtgc tcggcggcgg gcgccggaag cagccgctcg agcgcgttga
     7441 ggagcggcac cacgctcggc ttctcgaggg cgctcacgca cacgctgccc gcttggtgct
     7501 cgaacgcgtc gcgttcgctc ggctcgaggc ggtcgatctt gttgtacacg cggacacgcg
     7561 gcgtgagctc gaggccgagc tcggcgagga cctccgtcgt cgtctgcacg tgctcttcgt
     7621 gctcgggatc gctcacgtcg acgacttcga cgatgacgtc ggcgtcggtc gcttcttcga
     7681 acgtagcgcg gaacgccgcg aacaggtcct tgggcatccg gcggatgaac cccaccgtgt
     7741 ccgtcagcac caccgagcgt ccgttcggca gcatcaggcg ccgtgcgcgc gggtcgagcg
     7801 tcgcaaacag cttgtcttcg gcgagcactc cggcctgcgt cagcgtgttc agcaaggtgc
     7861 ttttgccagc gttcgtgtag ccgaccaacg cgacgacggg caaatcacct tcgcggcggc
     7921 gcttccggcg ctcgcgccgt tgcgcttcga gcgactcgag ctccttctcg agccggtgca
     7981 cgcgctcgcg cgcgcggcgc cgcccaattt cgagcttggt ttcgccgggg ccccggccgc
     8041 cgatgccacc cgtgaggcgt gacagcgaat cgtctttttg ctgcaagcgg gggagggcgt
     8101 acttcagctg cgcgagctcg acctggagct tgccgtcgcg ggactctgcg cgctgggcga
     8161 agatgtcgag aatgagctgt gtgcgatcga tgatcttcag atccgtctgc ttcgcgaggc
     8221 tgctcgcctg cgaagggctg agctcgcaat cgaagatcag cgtttcggcg tcgagatcga
     8281 tggctcgcat cacgatctcg tcgagctttc cgcggccgac tacgaaccgc ggatcggggt
     8341 gctcgcggac ctggatcacg ctgtcggcga cgtcgacccc ggccgtctcg gagaggctca
     8401 aaagctcggc gagctctgcc ttggcgcggt tcaatgcgcc cgcgcggcgc ttgtcggcga
     8461 cgtgcaccaa gatcgctcgg ccatctttgc cgcgcacgac gcgggcacgc cgcagttcgc
     8521 cgaactccgc ctcgagcgag gcgatgagct ctccgaaatt gggctgctct ttgccgtagg
     8581 gttgaggcgg aacgatttcg tatgggcgcg cgttcgcgtc gcgcgaaggg acgttgtacg
     8641 cccaggtgag cgcgcggggt tccccttcgg gggtcagctg aatcgccgcg accaggtcga
     8701 gccgaaggcg cgtcaggtcg atcaaatcgt cgcgcgtgag cggctcgccg aagaggtggg
     8761 tgtgcaccag ccggagagcg cggaaccgtc cctcggcggc gcggagacga ccgatgtctg
     8821 gcagcaaaag gccggtggcg tgaccgacga tgacgcagtc gacctctccc gaacggtgca
     8881 ccagggcccc gacctggcgc ttcgtttcgt tggatgcgtc gacgagcgaa cggacgagct
     8941 cgtgagtcac gattcggtcg ttcggcacgc ggcggcgata aattcgttcg agcgtcttga
     9001 gcgagctcgg gggcagcccc gtcgtattgc cgaatatttc cgtcatgagc gcgagagaac
     9061 gcctggaatc tggcacagat cgagggcccg gtcaccccgc ccgaacgatg ggctcggttc
     9121 tcgtcggcac gggcccgcac cgacgcgaaa tctcggggga tgcaggggct cgcaccagac
     9181 gatgtcgtaa cttcactgaa gcggtggtaa cgggcgaccc cgacatggct actttgacgc
     9241 ctctctcgct cgccgacgcc cagggcttgg cggcccgctt tggcctcgat ttggcggcgc
     9301 tcgagccgct tgcggcgggt tcggtgaact cgaacttcgc cctggagatg cgagacggaa
     9361 cccgctattt cgcgcggctg tacgaagagc aaccgctcgc gggcgcgcaa gtcgaggtcg
     9421 cgctcttggc cacactcgcg cgacgaggcg cgcgcgtcgt ggcgccatgc gaaaccgcgc
     9481 agggtgagcg ggtgttgctc cacgcgggta agccgtttgc agtctttcct tgggtggacg
     9541 gcgattggct ttgtttgcgg cgcgtcaacg cggctcactg cgaagcggtc ggcgcggagc
     9601 ttgcccgcgt gcacgccgcg agcagtgagg tgcctccgct ttcggagggg cggtttcgcc
     9661 ctcgggacat gctggcgcgg ctcgagcgag tcgagcgcga aggcccgccg cggctcgccc
     9721 ccgccctcgc cgagattcgc gcgcgctacg cggagtacct gccgaagcgc gatccggagc
     9781 tgccgcgcgg catctgtcac ggtgacttgt ttcgcgacaa cgtgctctgg caaggcccgc
     9841 gcatctcggc gttgctcgat ttcgagagcg tctactcggg tagcttcgcc tacgacctga
     9901 tggtgacggc gctcgcctgg tgctacacgg attcggtcgt cgtgccccac gtcgaagcga
     9961 tgtttcgtgg ctacgcaagc gtgcgcccgc tcagcgcggc agagcgcgag gcgttgccgg
    10021 tcgaaggcgc gctcgcatgc cttcgtttcg ctaccacgcg catcaccgat ttcgagctgc
    10081 gcgcggcgcc gggacaggcg cctgggcgtg actacaagcg tttcctcgag cggctccgcg
    10141 cgttgcagag cggagctttc gactcggcat ttcagagtct ggggtagacc ctcggcgaag
    10201 catattcagc aggatggcaa gaacatgacg gtagcgcgcg gcggcagact ccctcagtca
    10261 atcgatgcgg cgatggagcg cctcaacacg agcgtcgata tcgactccga gctttgggcg
    10321 gaagacattc aaggctcgat tgcgcatgcg cgcgggctcg cgcgggccgg cgttctcagc
    10381 gcgagcgagg ccgagacgat cgtgggtggg ctcgagagca tcgctgccga aatcaggaac
    10441 ggcacgttcg tctgggaccg agagaaagag gacgtgcaca tgaacatcga gtcgcgcctg
    10501 accgcgctcg tcggtagcgt ggggggtaaa ctccacaccg gccgctcgcg caacgatcag
    10561 gtcgcgacgg atcttcgtct ctactgccgc cgtcgagggc aagagctctt ggccgcgata
    10621 agcgacctag ggtcggcgat cctggagcga gcgcgcggtg agatcgacac gctgatgccg
    10681 gcgtatacgc acctgcagcg ggcgcagccg agccggctct cgcatcatct gatggcttgg
    10741 caagagttct tgttgcgcga ccggggccgt ctgcgcgacg cgctcacccg cgcgaacgag
    10801 tcgccgctcg gagcaggggc ggtcgccggc accgggtttt cgctggatcg ggccggggtc
    10861 gcgcgcgagc tcggcttcga gcgggcgatg cgaaactcga tcgatgccac cggcagccgt
    10921 gacttcctga tggaaatcgc ttctgcgctc gggattttgg gggttcacct gtcccgcatc
    10981 ggcgaggaaa tcgtgctctg gtcgacggtc gagttcggtt tcatgacgct gagcgacgcc
    11041 ttctcgacga gctcgtcgat gatgcctcag aagaagaatc cagacgtcgc cgagctcgtg
    11101 cgcggcaaag cggcgcgcct cgtcggctcg ctcacgagtc tgctagtcct cgagaagagc
    11161 ctcgccttcg gctatgggcg cgatctgcaa gaggacaagc gccccatctt cgacgccttc
    11221 gaagcggccg aagtcagtgt gcgggcgctg acgggcgcga tcctcaccgc cacgtttcag
    11281 cgcgagacga tgcagcgcgc gctcggccgt ggacacctct gcgcgacgga tctcgcggac
    11341 ttcctggttc agcgcgggct tccgttccgc gaggctcacc acgtcgtcgg tgggctcgtg
    11401 cgcgaagcgg aaagccgagg cgtgcagctg ggcgaactac cgggcgatgt gctcgaaggc
    11461 gcgcacgccg cgctcaaggg cccggagctc gcgagcgctc tcgacccggc cgcggccgtc
    11521 gagcgccgct ctctcgtcgg tggcccagcg cgcgcacgcg tgctcgccgc gatcagcgag
    11581 gccgagcgcg actggtcggc ctgagccgcg ggctgtcact tgaagcccga gtagacccaa
    11641 cgttcgcgcg tcggatcgct cgagctgaag gctcccgacg aggcgagcac gatcccagcg
    11701 acgagcgccg ttccggcccc caccacggca taggtgagcc agcgcggcag cggcgacttc
    11761 gcttcgttcg acacgttgcg cgtgggcggc ggagccgaag cgtgctccca cgcgccgcac
    11821 gagccggcat ggcagcgggc gagctcgagc tcgccgttcc gaactcgtgc ggcggcccaa
    11881 tcgctacatt gagtcctcgg gtgcgcgcgc ggcgacggct ctgcctcgac ggcgccatcg
    11941 aggtcatcgg cgctgcaggg cgtgaccgcc ggcaccggca agttcaccgt cgtcgccgtg
    12001 tctcccaccg tgacccaccc cgcccacacg agtcgttcgc ctcgcagcac gcgcacgtgg
    12061 tgctcgccgg accgactggc gacgttgagc gcgctcgctt cgccgtccca ctcgatgcga
    12121 gctctcgaat ctggcccggt cacggcgagt tcgcgcgctt cgggcaacgc gagcggtgtt
    12181 cccgcgcgcg tcgcgtctcg gaacgcctgg ccgcgttctc cttcgaggat cgccgcgcgc
    12241 cgcgcgagct cacgcgcgag gatcgggtcg tcgccttcga cgcgttcggc acgcgtcgtg
    12301 tgaagctcgg cgaggagcca tgcgccttgt ggaagctctg ggtgacttcg aatcaactgc
    12361 tcggcttcgg cgagcgcgcc gtcggcttct gccgcgcgga gcgcaccgat cgcagtcttc
    12421 gcggcaacga acaaggcttc gatccgccgt acgctttctg cctgctcggc gtccgcgcgc
    12481 gctccgagcg tatcgatttc atgcggcact tcgcgaaaat tgactgacca ccgagccgag
    12541 aaacgcgaga gactcggcag gttcgcaaag gagtccggca ccgacattgg ctccgtgagc
    12601 gtcgccgcgg acaggatcca aaggggaagc gttgggttca ccgagcggcc atgttagatt
    12661 caatcgttgg acgcggatag ggctcaggca gcgcgccaag cggccgttca accccttgcg
    12721 aggcactagt gcagctggat ttcgtcaacc gcgagctcac catcaagctc gtttattacg
    12781 gcccggctct gagcgggaag actacgaact tacaggcgtt acacaacgtc gccgctccag
    12841 gttcgagcgg gcgcttgatg accctcgaga ctcgcgacga ccgtacgctc tttttcgatc
    12901 tgctcccgct gacgttcaag agccaaggca gcctcgcggt gcggctcaaa gtgttcaccg
    12961 tcccgggaca ggtgatccac gcttcgacgc ggcgactcgt cgtgcaaggc gctgacggca
    13021 tcgcgttcat cgccgactcc cgagtctcgg agacgcagca caacgccgag gcattcctcg
    13081 acctcaaaca gaacctgaag gagcacggcc tcgccctccg caagatgccc ctcatcattc
    13141 aattcaacaa gcgcgacatg ccggcggtgc gcagcgacga ggagatcgct cagctcgccg
    13201 cgcagggacg cgaacccgtt tacatggcga tcgcgacgcg cggcc
//
LOCUS       DASTKS010000015         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_448000, whole genome shotgun sequence.
ACCESSION   DASTKS010000015 DASTKS010000000
VERSION     DASTKS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448000"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFQ35_00305"
     CDS             <1..232
                     /locus_tag="VFQ35_00305"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929091.1"
                     /translation="VTLIRSYLSFAGGTLVRKNLDAAAFVVYQSVRGTMLAYLLEEPA
                     GVNDAAIVEELSSMLTGYLVGGERDSSVEVTG"
     gene            complement(222..680)
                     /locus_tag="VFQ35_00310"
     CDS             complement(222..680)
                     /locus_tag="VFQ35_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929092.1"
                     /translation="MGSKSRHASLGVALRRLLLTALFCIACTKETVRSPTAEYLSGIQ
                     EAIERMQPTRMPTLIVTFDKDALEMGQCRRIAGETIVYLHLGAIADRTSSPTEARALI
                     AEVLAHELGHAELTCSDADHGLLLPHARRLSRDEARLHRSYVWQRAPVSQ"
     gene            805..1779
                     /locus_tag="VFQ35_00315"
     CDS             805..1779
                     /locus_tag="VFQ35_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929093.1"
                     /translation="MRWALVASALSAFLLVARVAEARPGSIDLTWNAPIGCPDREQVL
                     ASIRELMGESVVASDVAVRIDVARIDARLVLRLALKTEGSSAARTMESTDCEELARGA
                     ALVIAFAAGAEPPKPPSTQAPPTPAAKVQVAAPPRAPAMRYDVRVPVQPTAPAPRRGG
                     ALAWSALFGLDSGTFPRTSLGGALAARFDVPPFSGGFEALVLLPQDIDTATGGGRFWA
                     AALGLRPCASLSYARLRILPCAVADIEMMVAEGRGVTFREEGVVWFPRFGGGAELGYV
                     FTPKLSVVTGAWVLGAPSRPTFVIDGTVPIHTPGIWSVRWETGLEFAL"
     gene            2036..>2357
                     /locus_tag="VFQ35_00320"
     CDS             2036..>2357
                     /locus_tag="VFQ35_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9929094.1"
                     /translation="MTSPAHSILLDPAGSLTDRAAVALDFDSVYREHARFVWRSLSRL
                     GVLPEHVPDAMQDVFIVVYRKLPTFEGRSSVRTWLFGIAMRVASDWARRIRRHPSEPL
                     LDTLQ"
BASE COUNT          375 a          740 c          796 g          446 t
ORIGIN      
        1 ggtgacgctc atccgcagct atctgtcctt cgcgggcggt accctggtcc gaaagaacct
       61 ggatgcggct gcgttcgtcg tttaccaatc ggtgcggggt acgatgctcg cctacttgct
      121 cgaggagccc gccggcgtga acgacgcagc catcgtcgag gagttgagca gcatgctgac
      181 ggggtacttg gtgggcggcg agcgcgactc atcggtcgaa gtcactggct gaccggcgcg
      241 cgctgccaga cgtaagagcg gtgaaggcga gcttcgtccc gtgagagccg ccgcgcgtgg
      301 ggcaacagca agccgtgatc ggcgtcgctg cacgtgagct cggcgtgccc gagttcgtgt
      361 gcgagtactt cggcgatgag cgcccgcgcc tcggtcgggg agctcgtccg atccgcaatg
      421 gcccccaagt gtaaatacac gatcgtttcg cccgcgatgc ggcgacactg acccatctcg
      481 agggcgtctt tgtcgaaggt gacgatgagc gtcggcatgc gtgtcggctg cattcgctcg
      541 atggcctctt ggatcccgct caggtactcg gccgtcggag agcgtacggt ctccttcgta
      601 caagcgatgc agaagagagc cgtcaaaagt agtcgccgga gggcgacgcc gagagaggcg
      661 tgacgagact tcgagcccaa gacgatagtg tgtggccgcg gatcaccgcg atcggtggcg
      721 cagctcgtac gaaagatgga cgctcgcccc caggttcgta taattccgtc ctgcgtgtgt
      781 gcctcttctt atgcggggtc gaggttgcga tgggcgctcg ttgcaagcgc gctgtctgcg
      841 ttcctgctcg tcgctcgcgt cgcggaagcg cggccaggga gcatcgacct cacctggaat
      901 gcgccgatcg gctgccccga tcgagagcaa gtgctcgcgt ccatccgcga gctcatgggc
      961 gagtcggtgg tcgcgtcgga cgtcgcggtt cgaatcgacg ttgctcgaat cgacgcgcgc
     1021 ttggtgctgc ggctcgcctt gaagaccgag ggaagctcgg cggcccgcac catggagtcg
     1081 acggactgcg aggagctcgc tcgcggtgcg gcgctcgtga tcgcgttcgc agccggcgcg
     1141 gagccgccca aaccgccgag cacgcaagcc ccgcctactc ccgcggcgaa ggtccaggtc
     1201 gcagctccgc cccgtgctcc cgcgatgcgc tatgacgttc gtgtgcctgt tcagcccacg
     1261 gcgccggcac cgcgtcgcgg aggggccctc gcatggtctg cgctgttcgg gctcgacagc
     1321 ggaactttcc ctcgcactag cctgggcggc gcgttggcgg cgcgcttcga cgtccctccg
     1381 ttctccgggg gcttcgaagc gctagtgctc ttgccccagg acatcgatac cgcaaccggc
     1441 ggggggcgct tctgggcggc ggcgctcggc ctgcgtccgt gtgcgtcact gagctacgcg
     1501 cgcttgcgaa ttcttccctg cgccgtcgca gacatcgaga tgatggtcgc cgaagggcgc
     1561 ggcgtcacct tcagagaaga aggagtcgtt tggtttccac gatttggggg cggggcagag
     1621 ctcggctacg tcttcacccc gaagctttcg gtggttacgg gcgcatgggt gctcggggcc
     1681 ccgtcgcgac ccaccttcgt catcgacgga accgtcccta tccacacccc tggaatttgg
     1741 agcgtgcgct gggagacagg gctcgaattc gccctgtagc ggaggaggcg gtctcggaac
     1801 gcgcgcccac ggccgtttcc cgctaaaaaa atccgatctc ggcccgaaaa ggcaacacaa
     1861 acgaggacat ccaaccgtcc cttcccagcg ggagtgaaaa cacccgccgt tatgtcgaac
     1921 gtttcgatca gcgatggcca agaacttcac cgggcagcgc gtgtgagcgt gtcaaagtgg
     1981 tgggggtgtg attttggttc accgaaaccg gggcagcgcc gacactcaca gtcgaatgac
     2041 ctctccggcg cactccatcc tgctcgatcc cgccggatct ctgacggatc gagcagcggt
     2101 cgccttggac ttcgattcag tttatcggga gcatgcgcgt ttcgtgtggc gctcgctctc
     2161 tcggctgggg gttctcccgg agcacgttcc tgacgcgatg caagacgtgt tcatcgtggt
     2221 ctaccggaag cttccgacgt tcgaggggcg ctcttccgtg cggacctggc tctttggcat
     2281 cgcgatgagg gtcgcgagcg actgggcgcg ccgcattcgg agacacccga gcgagccgtt
     2341 gctcgacact ttgcaag
//
LOCUS       DASTKS010000016         7414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_671174, whole genome shotgun sequence.
ACCESSION   DASTKS010000016 DASTKS010000000
VERSION     DASTKS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671174"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFQ35_00325"
     CDS             complement(<1..272)
                     /locus_tag="VFQ35_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929095.1"
                     /translation="MLNRSALVISVLPLGAALFAASGCGSDSSSSGSNGGAGGVGGSK
                     GSGGAPIVSTPIDEVDFAVRFATALCQGEKSCCMSIGGVIDAPMDCS"
     gene            700..2184
                     /gene="ilvC"
                     /locus_tag="VFQ35_00330"
     CDS             700..2184
                     /gene="ilvC"
                     /locus_tag="VFQ35_00330"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF003557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9929096.1"
                     /translation="MTRQNYFNTLTMAQKLEQLGRCRFMDRSEFNGVERLRGKKVVIV
                     GCGAQGLNQGLNMRDSGLDVSYALRADAIEQKRASYTNATSNGFKVGTYEQLIPTADL
                     VCNLAPDKQHADVVSKVMPLMKKGACLAYSHGFNIVEEGQAIRKDLTVVMVAPKCPGT
                     EVREEYKRGFGVPTLIAVHKENDPNGDGLEIAKAYAAATGGDRAGVLESSFVAEVKSD
                     LMGEQTILCGMLQTGSLLCFDRMVAEGIDRGYASKLVQYGWETITEALKQGGITLMMD
                     RLSNPAKIKAFELAEELRAIMRPLFRKHQDDIITGAFSSTMMKDWDNGDAQLLGWRAA
                     TNDTAFERTPAGPDKISEQEYFDNAVLMVAMVKAGVELAFECMVEVGMKPESAYYESL
                     HETPLIANLISRKKLYEMNKVISDTAEYGCYLFSHAAVPLLKEFMAKQKTDVIGKPFS
                     AGSQQVDNRRLLTVNQAIEGHPVEKIGATLRGYMTAMKQIAVGG"
     gene            2220..3605
                     /locus_tag="VFQ35_00335"
     CDS             2220..3605
                     /locus_tag="VFQ35_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9929097.1"
                     /translation="MGEAEAALELTTVGPGLTLVTGHNGAGKSSAARALAARVEGAQL
                     LSAETQQAFYEAELANDDSNFQEAVDTGTTVGELLGDAGRRHPLFVAFRLEALWDRGY
                     RLLSSGESRKVLLLHAVVQAPSLLVLDDPFDGLDRAACAELAESIVRAAEALPLVVVG
                     TFAATELPFSLDSLREVVVIEARRIVFRGSPQAFLARALSRVTHRSKPPVHFGSFHAA
                     LELSPGVPLVDLRKGEVRYGDLVVFAGLDFRVATGAHTLIEGPNGSGKSSLLDMLTGD
                     HPQAYANELYLFGRRRGTGETVWDIKRNVGAVSGRLHRDYRVGGSIEEVLLSGLYDSI
                     GVYEKPEPSHRARARAWLDWLEVGLAPTASFRELSFGQQRLVLIARAAIKLPPLLVLD
                     EPTSGLDSENRAHVLELVESLCTQRLSTVLMVTHRVDERDFWQQRIGGAILSLPMKTN
                     SVAADQLEPSG"
     gene            3785..>7414
                     /locus_tag="VFQ35_00340"
     CDS             3785..>7414
                     /locus_tag="VFQ35_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929098.1"
                     /translation="MGGAPNAETTKAVLAGGGEMGALMRSIDWAHTAVGPVEAWPQSL
                     RTALSILLDTGFPMYIAWGRDFTQFYNDGYRPILGSTKHPTAMGISTRETFAEIWDII
                     GPMFEGVMQGKTTTVVDFLLPLDRHGFVEECYFIFSYSPIREEGGRVGGVLVTVTETT
                     QRVLTERRLKTTQALAAETRQAKTVSEACALAGHVLEQNPADLPLARIYLVEADGASA
                     RAAATTAHDALPPELGEVVSLESGPLRAAMNSGRAEGLEDIAVPSVGKLLALPITPQG
                     AERPIGLLVAGTSARLILDDPYRDFLGLVSSQIATAIAGAQIVEQAQARAEELARLDR
                     AKTAFFSNVSHEFRTPLTLMLGPTQEALHSPDHALRGDDLDTVYRNELRLLKLVNTLL
                     EFSRIEAGRVDASFAPTDLAALTTDLASAFRSATTRAGLELIVDCPPLREHVYVNPEM
                     WETIVLNLLSNAFKFTFEGSITVELAARESHVELRIRDTGVGIAAEDLGRIFQRFQRV
                     ERAAARTHEGSGIGLALVQELVAIHGGSIEASSELGKGAEFCVKIPLGSDHLPAPRVR
                     SGPSSTAVANAAGAALPFVEEALRWLPGGSEEVPKSGARTSLTQPRILIADDNADMRD
                     YLSRVLSARFQVRAVGTGDAALDAALEQRPDLVVSDVMMPGLDGFQLIAALRGREETR
                     TVPVILLSARAGEEARVDGLKAGADDYLVKPFSARELVARIDSQLVRAKLRSLEEAHA
                     LRVADVFAHAPVGVAILRGPAHVYDFANERYRDLVGGRPLLGKPIREALPELEGQGIF
                     ELLDRVFETGQPHVGRSLRLLLKRRANGPEETYFDFTYQPLVDDEGHTTGITVVAVDV
                     TAITAARRQAESANRAKDEFLAMLGHELRNPLAPILTALQLMQLRGVVGGERERTVIE
                     RQVKHLMGLVDDLLDVSRIARGKVQLKRELVDIGEVTARAIEMTSPLIDDRRHVLDVA
                     APRGVIVYGDASRLAQVVANLLTNAAKYTDPGGRIAVTIEPVGSDMRLSVKDSGRGID
                     PEMLPRVFDLFSQERQEIDRSQGGLGLGLAIVKSLVEAHGGSVEVRSEGKGRGAEFIV
                     GLPLAELRANEAESLPKRGSSTANAAEGLRILIVDDNADAAEMLADSLRALGHRTQTA
                     FDGLRALEIAPEFRPEVALVDLGLPVMDGFEVARALVSSVGEQITLVAVTGYGQESDR
                     SATREA"
BASE COUNT         1363 a         2453 c         2372 g         1226 t
ORIGIN      
        1 gagcaatcca tcggcgcatc gatgacgccg ccaatgctca tgcagcatga cttttctcct
       61 tggcagagcg cggtcgcgaa gcgaaccgcg aagtccacct cgtcgattgg tgtcgaaacg
      121 atgggtgcac cgcccgagcc cttggagcca ccaacgccgc ccgcgccgcc gttgctccct
      181 gaagacgagg agtcgctccc gcaacccgat gcggcgaaaa gcgccgcgcc gagagggagc
      241 accgagatga ccaaagccga acgattcaac acgaccatcg aacctgcccg atgccagtac
      301 gccccgagta cgtcaacacc aactcatcac tcttccggaa atgactagcc gcttgcgagt
      361 aaccaaagcc cggccggctt cgcggctcgc gcgaatcgcg atatcttcgt tctcgcagtc
      421 gcgaggccgc acggacccaa gcttcgcgcg acgccgcgtg cacgaagcga cgtttgccgc
      481 ttcagcggga gggaggcccg tcccttcgaa ggtgacggcg cggtttctcg agatcgaggg
      541 ctgaccggcg gcgggccccc tcgccgcgcc tgttgcgttt tttgcaacct ctcgtcccgt
      601 taatagcacc aggcgcaaca ttggctgacc ggcttgggcg agctatcttc tcggtcctcg
      661 atcgccgata ggcgtcagaa cccgagaaag aggcgcagca tgacccgtca gaactacttc
      721 aacaccctca cgatggcgca gaagctggag cagctcggcc gatgtcgctt catggaccgt
      781 agcgagttca acggtgtgga gcggctgcgc ggcaagaagg tcgtcatcgt cggctgcggc
      841 gcgcagggcc tgaaccaggg cctcaacatg cgcgactcgg gcctcgacgt cagctacgcg
      901 ctccgcgccg acgccatcga gcagaagcgc gcgagctaca ccaacgcgac cagcaacggc
      961 ttcaaggtgg gcacctacga gcagctcatc cccaccgccg atctggtctg taacctggcg
     1021 cctgacaagc agcacgccga cgtggtctcg aaggtgatgc cgctcatgaa gaagggcgcc
     1081 tgtctcgcct acagccacgg cttcaacatc gtggaagagg gccaagccat ccgcaaagat
     1141 ctgaccgtcg tcatggtggc gccgaagtgc cccggcaccg aggtgcgcga agagtacaag
     1201 cgtggcttcg gcgtgccgac gctcatcgcc gttcataaag agaacgatcc gaacggcgac
     1261 gggctcgaga tcgcgaaagc atacgcggcc gcgaccggtg gtgaccgcgc cggcgtgctc
     1321 gagtcgagct tcgtcgccga ggtgaagagc gacctgatgg gcgagcagac gatcctgtgc
     1381 ggtatgctgc agacgggctc gctcttgtgc ttcgaccgca tggtcgcaga gggcatcgat
     1441 cgcggctatg cgagcaagct cgttcagtac ggctgggaaa ccatcaccga ggccctcaag
     1501 cagggcggca tcacgctgat gatggatcgc ctgagcaacc ccgccaaaat caaggctttc
     1561 gagctcgccg aagagctccg cgccatcatg cgcccgctgt tccgcaagca ccaagacgac
     1621 atcatcacgg gcgcgttcag ctcgaccatg atgaaagatt gggacaacgg cgacgcgcag
     1681 ctcctcggct ggcgcgcagc caccaacgac accgcgttcg agcgcacgcc ggccggcccc
     1741 gacaagatca gcgagcagga gtacttcgac aacgcggtgc tgatggtcgc gatggtcaag
     1801 gcgggcgtcg agctcgcctt cgagtgcatg gtcgaagtcg gcatgaagcc ggagtcggcg
     1861 tactacgagt cgttgcacga gacgcccctc atcgcgaacc tcatctcccg caagaagctc
     1921 tacgagatga acaaggtgat cagcgatacg gccgagtacg gctgctacct cttcagccac
     1981 gccgccgtgc cgctcctcaa agagttcatg gcgaagcaaa agaccgacgt gattggcaag
     2041 ccgttctcag ctggcagcca acaggtcgac aaccggcgct tgctcaccgt aaaccaagcc
     2101 atcgagggtc acccggtcga gaagatcggc gcgacgctcc gcggctacat gaccgcgatg
     2161 aagcaaatcg ccgtcggcgg ctgagaccgc gatagcgagc gctgacgagc tcgacctcca
     2221 tgggtgaagc cgaagccgcg ctggagctca cgaccgttgg tcccggactc acgctggtca
     2281 cgggacacaa cggcgcggga aaatccagcg cggcccgagc gctcgcggcg cgcgtagaag
     2341 gcgcgcagct gctatcggcc gaaacacagc aggcctttta cgaggccgag ctcgcgaacg
     2401 acgacagcaa tttccaggag gccgtcgata cgggcactac cgttggcgag ctgctcggcg
     2461 atgcgggccg ccgccacccg ctcttcgtcg cgtttcggct cgaagccttg tgggatcggg
     2521 gctaccggct actttcgagt ggtgagtcgc gcaaggtctt gctgctgcac gccgtcgttc
     2581 aggcgccgtc gctgctggtg ctcgacgatc cgttcgatgg cctagatcgc gcggcgtgcg
     2641 cggagctcgc ggagtccatc gttcgagccg cggaggcact tccgctcgtg gtcgtgggga
     2701 cgttcgccgc gaccgagctc cccttctcac tcgactcgct tcgcgaagtg gtggtgatcg
     2761 aagcccgacg aatcgtattc aggggcagcc cccaggcgtt tctagcgcgg gcgctgagcc
     2821 gcgtgacaca ccggagcaag ccccccgtgc acttcgggag cttccacgct gcgctggagc
     2881 tcagccccgg cgtgccgctc gtcgatctcc gaaaaggcga ggtgcgttac ggcgacctgg
     2941 tcgtctttgc agggctcgat ttccgagtcg cgaccggagc acacactttg atcgaggggc
     3001 ccaacggctc cggcaagtcc agcttgctcg acatgctcac tggcgatcat ccccaagctt
     3061 atgccaatga gctttacctg ttcggtcggc gccgcggcac cggcgagacg gtttgggaca
     3121 tcaagcgaaa cgtcggcgcc gtatcgggtc gattgcaccg cgactaccga gtgggcggta
     3181 gcatcgaaga ggtcctcctc agcgggctct acgacagcat cggtgtttac gagaaacccg
     3241 agccgagcca ccgagctcgt gcccgcgcct ggctcgactg gctcgaggtc ggattggcac
     3301 caaccgcttc gtttcgcgag ctttcgttcg ggcaacagcg gctcgttttg atcgcgcgcg
     3361 ctgcgatcaa gcttccgcca ctcttggtgc tcgacgaacc aacctccggc ctcgactccg
     3421 aaaaccgagc gcacgtcctc gagctcgtgg agtcgctctg tacgcaacgg ctcagcaccg
     3481 tgctgatggt cacgcatcgc gtcgacgagc gagacttttg gcagcagcgc atcggcggag
     3541 ccatcctctc gttgccgatg aagacgaact cggtcgcggc agaccaactc gagcccagcg
     3601 gatagcgcga ctcgagccct gagccgagag actcgacagc tcgggtcacg agcctgccag
     3661 ttacgaacat gcggttttcg agaggaaatc tcggcgaagt ccgggtgtgg ccgttttgcg
     3721 tcaggaggca ggacctacct tccttgatgt ccgagctcct tggtgcgaaa gcggtgagat
     3781 gaccgtgggc ggtgctccga acgcagagac aaccaaggca gtgctcgccg gcggcggcga
     3841 gatgggcgca ctcatgcggt ccatcgactg ggcccatacc gcggtcggcc ccgtagaggc
     3901 atggccccaa agccttcgca ccgcgctgag catcctgctc gacaccggct tcccgatgta
     3961 catcgcgtgg ggccgcgact tcacccagtt ttacaacgac ggctatcgac ccatcctcgg
     4021 ctcgacgaag cacccgaccg cgatgggcat cagcacgcgc gaaaccttcg cggaaatttg
     4081 ggacatcatc gggcccatgt tcgagggcgt catgcagggc aagacgacga ccgtcgttga
     4141 ttttctgctg ccgctcgatc gtcacggctt cgtggaagag tgctacttca tcttttctta
     4201 cagccccatc cgcgaagagg gtggacgcgt cggtggtgtg ctcgtcaccg tgaccgaaac
     4261 gactcaacgc gtcctcaccg agcggcgctt gaaaacgacg caagctctcg cggccgaaac
     4321 gcggcaggcg aagacggtct ccgaagcgtg cgcgctggcg ggtcacgtgc tcgagcagaa
     4381 cccagccgat ctgccgctcg ctcgaattta cctggtcgaa gccgatggag cgagcgctcg
     4441 cgcagcggcg acgaccgcgc acgatgcact cccgcccgag ctcggtgaag tcgtctcgct
     4501 ggagagcgga ccgctccgag cggcaatgaa ctccggccgg gccgaggggc tcgaagacat
     4561 cgccgtgccc tccgtcggca agctcctcgc gctgcccatc acgcctcaag gcgcggagcg
     4621 cccgataggt ctgctcgtag cgggcacgag cgcgcgcctg attctcgacg atccttatcg
     4681 agatttcttg ggactcgtct cgtcgcaaat cgcgaccgcg atcgccggcg cgcaaatcgt
     4741 cgagcaagcg caggccaggg ccgaagagct cgcgcggctc gatcgagcca agaccgcctt
     4801 cttcagcaac gtgagccacg agttcagaac cccgctcacc ttgatgttgg ggccgaccca
     4861 agaagcgttg cattcaccgg atcacgcgct gcgcggcgat gatctcgaca ccgtttacag
     4921 aaacgagctc cgcttgctca agctcgtcaa taccctgctc gagttttccc gcatcgaggc
     4981 gggccgggtc gacgcgagct tcgcgccaac cgacctcgcc gcgctcacga ccgacctcgc
     5041 gagcgctttt cgttccgcga cgacgcgcgc ggggctcgag ctcatcgtcg actgcccacc
     5101 cctccgagaa cacgtctacg tcaacccgga gatgtgggag acgatcgttt tgaacctcct
     5161 ctcgaacgcc ttcaaattca cgttcgaagg ttccatcacg gtagagctcg ccgctcgcga
     5221 gagccatgtc gagctccgca tccgtgacac cggggtcggc atcgcggcgg aggatctcgg
     5281 gcgcatcttc cagcgcttcc aacgggtcga acgcgctgcc gcgcgcacgc acgagggctc
     5341 gggcatcggc ctcgcgctgg tccaagagct cgtcgcgatt cacggcggca gcatcgaggc
     5401 gagcagcgag ctcggcaaag gtgccgagtt ttgcgttaag atccccctcg gatccgacca
     5461 tttgccggcc ccgcgggtgc ggagcgggcc ttcgagcacg gcggtcgcga acgcggccgg
     5521 cgcggcgctg cccttcgtgg aagaggcgct tcgttggcta cccggcggct cggaggaggt
     5581 gcccaagagc ggcgcgcgga cctccctaac ccagccccga atcttgatcg ccgacgacaa
     5641 cgccgacatg cgcgactact tgagccgagt gctctccgcg cgcttccaag ttcgcgccgt
     5701 gggtaccggc gacgcggccc tcgacgcggc cctcgagcaa cgacccgatc tcgtcgtgag
     5761 cgacgtgatg atgcccggcc tcgacggctt tcaactcatc gccgccttgc gcgggcgcga
     5821 agagacgcgc accgtgcccg tcatcttgct ctcggcgcgc gcgggcgaag aagcgcgcgt
     5881 cgacggcttg aaggcgggcg ccgacgacta cctcgtcaag cccttttcgg cgcgcgagct
     5941 cgtggcccgc atcgatagcc agctcgtccg cgccaagctg cgttccctcg aagaggctca
     6001 cgcgttgcgc gtcgccgacg tgttcgcgca cgcgccggtc ggcgtcgcca tcttacgcgg
     6061 ccctgcgcac gtgtacgatt tcgcgaacga gcgctatcgc gacctcgtcg gtggccgccc
     6121 gttgctcggc aagccgattc gcgaagcttt accggagctc gaagggcagg gcattttcga
     6181 gctcttggat cgcgtcttcg agacgggcca gccgcacgtc ggcaggtcgt tgcgcctctt
     6241 gctcaagcgc cgcgcgaacg ggcccgaaga gacctatttc gatttcacct atcagccgct
     6301 ggtcgacgac gaagggcaca cgacggggat cacggtggtc gcggtcgacg tgaccgccat
     6361 cacggccgcg cgccgacagg ccgagtcggc aaaccgagcc aaagacgagt tcttggcgat
     6421 gctcggccac gagctccgca acccgctcgc gccaattctt accgcgctcc agttgatgca
     6481 actgcgcggc gtcgtgggcg gcgagcgcga gcgaacggtg atcgagcggc aggtgaagca
     6541 cctgatgggc ctggtcgacg acttgctcga cgtttcgcgc atcgcgcgcg gcaaggtcca
     6601 gctcaagcgt gaactcgtcg acatcggcga ggtgacggcg cgcgcgatcg aaatgacgag
     6661 cccattgatc gacgatcggc ggcacgtctt ggacgtcgcg gcgccacgcg gtgtcatcgt
     6721 gtacggagac gcatcgcggc tcgcgcaagt cgtcgctaat ttgctcacga acgcggccaa
     6781 gtatacggat ccgggcgggc gcatcgccgt tacgatagaa cccgttggca gcgacatgcg
     6841 tctgagcgtg aaagacagcg gtcgcggtat cgatcccgag atgttgccgc gcgtgttcga
     6901 cctcttctcg caggaacgac aggagatcga tcgctcacag ggcgggctcg ggctcggact
     6961 cgcgattgtg aaaagcctcg tcgaggcgca tggcggcagc gtcgaggtgc ggagcgaggg
     7021 caaggggcgc ggcgcggagt tcatcgtcgg gctcccgctg gccgagcttc gcgccaacga
     7081 ggcggagagc ctgcccaaga gagggagctc taccgccaat gcggccgagg gccttcgcat
     7141 cctgatcgtc gacgacaacg ccgacgccgc ggaaatgctg gctgactcgc tgcgagcatt
     7201 gggacatcgc acccagaccg ctttcgatgg cctgcgcgct ctcgagattg ccccggagtt
     7261 ccggcccgaa gtcgcgctcg tcgacctcgg gctgccggtg atggacggct tcgaagtcgc
     7321 tcgcgcgctc gtgtcgtcgg ttggcgaaca aatcacgctc gtcgcggtga ccggctatgg
     7381 ccaagagagc gatagaagcg ccacgcgcga agcc
//
LOCUS       DASTKS010000017         5328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1786241, whole genome shotgun sequence.
ACCESSION   DASTKS010000017 DASTKS010000000
VERSION     DASTKS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786241"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(638..1423)
                     /gene="uppS"
                     /locus_tag="VFQ35_00345"
     CDS             complement(638..1423)
                     /gene="uppS"
                     /locus_tag="VFQ35_00345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET9929099.1"
                     /translation="MKDGNNGSNRVPQHVAIIMDGNGRWAKQRGLERVEGHAEGARAV
                     RDAVETSTRVGIKYLTLYAFSVANWARPRGEVEALMRLLVEFAKKERAELRQNGIRVN
                     VIGALDDLPTSTRHAVEDLIRYTADGDRMTLTLALSYGGRQDIVEAARSLAVRARAGL
                     VLPEEIDENFFHKQMTTHDLPQVDLLIRTGGETRLSDFLLFESAYAELLFLPIMWPDF
                     RPQTLLDAMQFYADKERRFGMISEQIQGRIPIQAFDPVDRIQN"
     gene            complement(1704..3083)
                     /locus_tag="VFQ35_00350"
     CDS             complement(1704..3083)
                     /locus_tag="VFQ35_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929100.1"
                     /translation="MLRLVVVAAAAIGLILFVAFVVSRLLGTRTRGMSIRMQVFLALA
                     VIVGAFAFGLGVMVIDRIEARAVGLARQAAEDEAAAIAGMMAGEMDRSGVSLGSIAAR
                     LELERARGADLRMVLREADGRIVFPRNANAELEPGMVVVDVPLVVRGERRGSVRVVKP
                     TVIMRNLLADMAPTVLVISLVLGAAAALAAAWIGREIAQPIEALGAFSERVSTGERSA
                     APPPVFGREVMRLSRSLDSMRRQLEGRPFVETFAADLSHELKNPVAAIRASAEVLEDS
                     ALEEPEQARRFVARIREATERIERLLSELLSLARIEARGAEAFEPLDLRALVERTVEA
                     FGEEAARVEFASQGDLRVRGDAVWLSRAVSNLIDNALAHSPAGSKVRVQLERREQVEL
                     SVQNQGEIPKYVRERVFRRFVTTRGDKGGSGLGLAIVRAVAEAHGGQAELVQPGPDAV
                     IFRIKLPAA"
     gene            complement(3092..3778)
                     /locus_tag="VFQ35_00355"
     CDS             complement(3092..3778)
                     /locus_tag="VFQ35_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9929101.1"
                     /translation="MSQRILVVEDEPAIAESVAYALTRDGFQVTIAATGSAAEKQVGD
                     VELVVLDLMLPDDSGFNLIAGWRKGGLSTPIIVLSSRDDEADRVAALEAGADDYVTKP
                     FSPREVVARVRAVLRRAPALKSGSVEVSIPLRVDPSTRRAFVDAKELELTRVEFDLLA
                     CLLETPGRVFTRSELIKRVWGDGFALTDRTVDSHVKALRRKVAEIGAAPSIIETVRGV
                     GFRVTDTPEE"
     gene            3991..4488
                     /locus_tag="VFQ35_00360"
     CDS             3991..4488
                     /locus_tag="VFQ35_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9929102.1"
                     /translation="MKTLLVLRHAKANRESDSAADHDRTLAKRGVKAAERIGALLRDE
                     KLLPELVLSSTAARARSTAELVAREAGVTDKLVSFLPQLYLAEPPAYIEALRKLPREA
                     ERVLVVGHNPGIETLVYRLTGETEHMPTAALAVCELPIDDWSELSGATSGHLARVVRP
                     KGLDE"
     gene            complement(4571..>5328)
                     /locus_tag="VFQ35_00365"
     CDS             complement(4571..>5328)
                     /locus_tag="VFQ35_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9929103.1"
                     /translation="RDDSTIFSWELGNEPRCKGTGTGASGWDNQTIVQWADEMSRYIH
                     SLDPNHMVSVGDEGFLDGGGEHWAYRANDGVDHEALTALSGVDFGTFHSYPEDWGATL
                     AWGDRWIVDHERVARRLGKPTILEEYGVKVTRNEQAVIVKNLDVRLKTYRRWNELTLE
                     RGGNASMIWMLGGKDTLYPAPNGLYKDYDGYTVFRGDESTSLLADIAKDFLEAAPACR
                     LGAQPSGAPSPFVRAQRSRASEPVAMGWGVSGG"
BASE COUNT          927 a         1777 c         1741 g          883 t
ORIGIN      
        1 catcgagcgc atcctagctg cacttgaaac cgacgcgcgg ctcgacaaac tcgttttcga
       61 tcatccggat gtcatcgaag aactgagacg catcgtcctt cagcgaagcc ctcgaagtag
      121 cgggacgttg cctcggggag aaccaaactc gagccgcgat tggagaaccg caactcgagc
      181 gaaaagccgc ttctcgaagt cgcccgaggc gcgctcctcg cgcacccgac aagccgacgt
      241 tggaggtgcg ccggcaaggc aagagttgca gggctcggac caacccgtgc gagagtctca
      301 cccgcctcta tccccttcgg agccgcctcg gagtggtgag agtgcttact cgacgcgacc
      361 cagtgcgcca tcgagtgcct cttcgaaccc tccgcccgtg tcgtccaatc accctggcgc
      421 ggtctcggcg atccacgcga cccaggccat ctcagcggct tccggagacg acaacgaacc
      481 gccgctctac cgcgaaaaga agtagctccc cacgtggcag agcacaccct cggcatgcat
      541 cctccccctg acggcgcgta gcgcagcgta gcgccggatt tgggggaggc cgccgcccgc
      601 agcgcgcagc gcgaggacgg acggggtggg ggcaacttca gttctggatt cggtcgaccg
      661 ggtcgaaagc ttggattgga attcgcccct gaatctgttc cgagatcatg ccgaagcggc
      721 gctctttgtc ggcgtagaac tgcatcgcgt cgagcagcgt ttgggggcgg aagtcgggcc
      781 acatgatggg caggaacaag agctccgcgt acgccgactc gaacaagaga aaatcgctga
      841 gccgcgtttc accgccggtg cggatgagca gatcgacttg cggcaggtcg tgagtcgtca
      901 tctgcttgtg gaagaagttc tcgtcgatct cttccggcag cacgaggccc gcacgggcgc
      961 gcacggcgag cgagcgagcg gcttcgacga tgtcctggcg accaccgtac gagagggcga
     1021 gcgtgagggt catgcgatcg ccgtcggcgg tgtagcggat gaggtcttcg acggcgtggc
     1081 gagtcgacgt cggtaggtcg tcgagggcgc cgatcacgtt cacgcgaatg ccgttctgtc
     1141 gcagctcggc gcgctccttc ttggcgaact cgacgagcaa gcgcatgagc gcttcgacct
     1201 cgccgcgcgg gcgcgcccaa ttggcaacgc tgaaggcgta gagtgtgagg tacttgatgc
     1261 cgactcgcgt cgaagtctct acggcgtcgc gaacggcgcg cgccccttcg gcgtgtccct
     1321 cgactcgctc gagccctcgc tgcttcgccc aacggccgtt gccgtccatg atgatggcga
     1381 catgttgcgg aacgcgattc gagccgttgt taccgtcttt cacactaagc tccgattcaa
     1441 tgctggcggc cttgcgggcg cctctgcgaa ccccttcctc ccacatatca acgtccactg
     1501 cgtgaggagc tttcgaggag aggcgtacga aaccctggtg ctcgggccgc acgggggcgt
     1561 taaaccccag taatttcgct aagatcgaca tacatcagaa attgggaagg aatctgggcg
     1621 acccgccggc cgcaaacgcg cggccgctcg atcgagttgt gactcccggg agtgacgaag
     1681 ctgcgcggct gacggacgtg cgttcaggca gccgggagct tgatgcggaa gatcacggcg
     1741 tccggacctg gttggacgag ctcggcttgt cctccgtgcg cctcggcgac ggcgcgcacg
     1801 atagcaaggc cgagcccgga gccgcccttg tcgccacgcg tcgtgacgaa gcgtcgaaag
     1861 actcgctcgc ggacgtactt ggggatttcg ccctggtttt gaaccgagag ctcgacctgc
     1921 tcgcggcgct ccagttggac gcgcactttg ctgccggcag gcgagtgcgc gagcgcgttg
     1981 tcgatgaggt tcgagacggc gcgcgaaagc catacggcgt cgccgcggac gcgtaggtca
     2041 ccttggctcg cgaattcgac gcgcgccgcc tcttctccga acgcctcgac cgtgcgctcc
     2101 accaaggcgc gtaagtcgag cggctcgaat gcttcggcgc cgcgcgcctc gatgcgcgcc
     2161 aagctgagaa gctccgagag gagccgctcg atgcgctcgg tggcctcgcg gatgcgggcc
     2221 acgaagcgcc gcgcctgctc cggctcctcg agcgcgctgt cttcgagcac ctctgcgctc
     2281 gcgcggatcg cggcgaccgg gttcttgagc tcgtgcgaca gatccgcggc gaaggtctcg
     2341 acgaagggtc gcccctcgag ctgacgtcgc atcgagtcga gcgaccgcga gagccgcatc
     2401 acttcgcggc cgaacacggg tggtggtgct gccgagcgct cgccggtgct gacgcgctcg
     2461 ctgaaggcgc cgagcgcctc gatcggctgc gcgatttctc tgccgatcca cgcggcggcg
     2521 agcgcggccg cggcgccgag cacgaggctg atgaccagca ccgtcggggc catgtccgcg
     2581 aggaggttgc gcatgatcac ggtcggcttg acgacgcgga cgctgccgcg gcgttcaccg
     2641 cgcacgacga gcggcacgtc gacgacgacc atgccgggct cgagctcggc gttggcgttg
     2701 cgagggaaaa cgattcgacc gtctgcctcg cgcaggacca tgcgcaagtc ggcgccgcgc
     2761 gcgcgctcga gctcaagccg agccgcgatg ctgccgaggg atactccgct gcgatccatc
     2821 tcgcccgcca tcatccccgc gatggccgcc gcctcgtcct cggcggcttg acgtgcgagc
     2881 ccgaccgccc gcgcttcgat gcggtcgatg accatgacgc cgaggccgaa cgcgaaggct
     2941 ccgacgatga ccgcgagcgc caagaacacc tgcatgcgga tgctcattcc gcgcgtccgc
     3001 gtgccgagca gacgcgacac gacgaacgcg acgaatagaa tcaggccgat cgcggcggcg
     3061 gcgacgacca ccaaacgaag catgagcccc cctattcctc gggagtatcg gtcacgcgga
     3121 agccgacgcc ccgcaccgtc tcgatgatgg aaggggccgc gccgatctcc gcgaccttac
     3181 ggcggagcgc tttcacgtgg gagtccaccg tccgatccgt gagggcgaag ccgtcgcccc
     3241 aaacgcgttt gatgagctcg ctgcgcgtga acacgcgacc gggtgtctcg agcaaacacg
     3301 cgagcaggtc gaactcgacg cgcgtcagct cgagctcttt ggcgtcgacg aatgcgcggc
     3361 gcgtgctcgg gtccacccgc aaaggaatgc tcacctcgac gctaccggac ttcagcgccg
     3421 gagcgcgccg caacaccgcc cggacgcgag ctacgacctc gcgcggagag aacggcttgg
     3481 ttacgtagtc atcggcgccg gcctccagcg ccgcgactcg atccgcctcg tcgtcgcgac
     3541 tcgacagcac gatgatggga gtcgagagcc cgcctttgcg ccagcccgcg atcaagttga
     3601 agccgctgtc gtcgggcagc atcaagtcga gaacgacgag ttcgacgtca cccacctgct
     3661 tctccgcggc gcttccggtg gcggcgatcg tcacctgaaa gccgtcccgc gtgagcgcgt
     3721 acgcgaccga ttcggcaatg gcgggctcgt cctcgacgac taagatgcgt tgactcattc
     3781 ggtgaacaat ctggggcgtg acgagcgcgt gacggctcga ctatagcgcc ccaggtgcgt
     3841 cgtgcccacg gcgccggcac ggctcaggag ccgcagggac tctgcgtccg gaacgctttc
     3901 cgagaccaga ggcgggacgg ctcgttacca cgaggaccgc taggatcctt cgcattgcgt
     3961 catgcgggcg gagcggggat gctccccgtc atgaagactc tcttggttct gcggcacgcc
     4021 aaggccaatc gcgagagcga ctccgcggcg gatcacgatc ggactttggc gaagcgcggc
     4081 gtgaaggcgg ccgaacgcat cggcgcgctc ctgcgggacg agaagctcct tcccgagctc
     4141 gtgctctcgt ccaccgctgc tcgtgcccgc agcaccgcgg agctcgtcgc gcgggaggcc
     4201 ggcgtcaccg acaagctcgt cagcttcttg ccgcagcttt atctcgccga gccgccggca
     4261 tacatcgaag cgcttcgcaa gctaccccgc gaagccgagc gcgtcctcgt cgttgggcac
     4321 aatccgggca tcgagacgct cgtttaccga ctcacgggcg agacggagca catgcccacc
     4381 gcggcgctcg cggtgtgcga gctccccatc gacgactggt cggagctcag cggcgcgacc
     4441 agcggtcacc tcgcacgagt cgtacgcccg aaggggttgg atgaatgagt tgcggaggaa
     4501 cggtctcgtt gttcggcctg ctgcgctcgt ccaccttgct gtgagttgaa gcaaggcgcc
     4561 cgaccagact tcatccgcca gagactcccc aacccatcgc gacaggctcc gaagctctgg
     4621 atcgttgcgc gcgaacgaac ggcgacggcg cgccgctcgg ttgcgcaccg aggcgacacg
     4681 ccggtgccgc ctcgaggaag tctttggcaa tgtctgcgag caacgaagtg ctctcgtcgc
     4741 cgcggaacac ggtgtagccg tcgtagtcct tgtaaaggcc gttcggcgcc gggtagagag
     4801 tatctttacc gccgagcatc caaatcatgc tcgcgtttcc gccgcgctcg agcgtcagct
     4861 cgttccaacg ccggtacgtc ttcagccgaa cgtcgaggtt cttcacgatg acggcttgct
     4921 cgttgcgcgt caccttgacg ccgtactctt ccaaaatagt gggcttgcca agccgccgcg
     4981 cgacgcgctc gtgatcgacg atccagcggt cgccccaggc gagcgtggcg ccccaatcct
     5041 ccggatagga gtggaaagtg ccgaaatcga cgccggacaa agccgtgagc gcctcgtgat
     5101 cgacgccgtc gttcgccctg taggcccagt gctcgccgcc accatcgagg aagccttcgt
     5161 caccgaccga aaccatgtga ttcgggtcga gtgagtggat gtagcgcgac atctcgtcgg
     5221 cccactgcac gatcgtttgg ttatcccatc cggaagcgcc agtgcccgtc cctttgcagc
     5281 gcggctcgtt cccgagctcc caagagaaga tggtggaatc gtcccgat
//
LOCUS       DASTKS010000018         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_225772, whole genome shotgun sequence.
ACCESSION   DASTKS010000018 DASTKS010000000
VERSION     DASTKS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225772"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..2595)
                     /gene="ppk1"
                     /locus_tag="VFQ35_00370"
     CDS             complement(328..2595)
                     /gene="ppk1"
                     /locus_tag="VFQ35_00370"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET9929104.1"
                     /translation="MSSAEPSRVDASRTPLSLPVETPVGVVPTRLDDPALYLNRELSW
                     LEFNARVLAEAADPAVPLYERLKFVAIFSSNLDEFFMVRAAGLQAQLYGEVEEVPPDG
                     QTPEQQLTSIATRVHELIKETYGIWNSTLRKELRAAGLAVVSPDELNQKEQAAIDEHF
                     VKNVYPVLTPIAIDPVHPFPHVRNKSINVGLMFEKRLHSTEPSFGLVQVPTMLPRFIR
                     VPVEGTRRAFVSLEDLIFRHTRMIFPQLKVIGQYAFRVTRNFDIELDEEEGEDLLLTL
                     QAELRRRERGNAVRIEVSGNAPEESIQWLCRELDLNPKTDVYLVDGPLFLGDMTVLTS
                     GDERRDLRDEAFTPQYVPPLRDTEDFFESIAERDVLLHHPYESFEAVIDFLWQAAEDP
                     RVLAIKQTLYRTGGESPLVKALQRAAELGKQVTALVELKARFDEESNIQWARALERSG
                     VNVMYGLLGFKTHAKVLMVIRRERNGLRRYVHLATGNYNQQTARLYEDISLFTAREDY
                     GEDATAFFNMLTGYSAPVRWNHWIVAPLGLHEAVLGLIARETAHALSGRPAHIIAKMN
                     SLVDADVIAALYAASQAGVEVDLLVRGICCLRPGVPGISERIRVKALIDRFLEHARIF
                     HFKNGGNEEVYCSSADWMPRNFRRRVEVMFPVLDPDLKTRVVTEILQTMREDNCKSWV
                     LRDNGKYERERPSEGATPRRSQQRFIEMARERARGNEAILGLSKAVPAPTPRALDKLR
                     RHGGKKRKRRHREEQ"
BASE COUNT          527 a          981 c         1019 g          592 t
ORIGIN      
        1 ccatcaggcg aatcaacagc gtcgctcttg gcgcgtgcgt atcgagcagc cttccaaagg
       61 tatctggtcg tgcagagatc agcgtcatgt tcgcgctccg tccatcgtcg cattgggggg
      121 gccgtaactc gcccgagtca gccgtttcaa ctgacgggac gcagtaccga cgggatgcag
      181 tatagaattc gccgacccgg agcagaagcg tcgctttgcg ccggttccac gagaacggtg
      241 cagagaagca ccccctcatg cctcgagctc gagtattcgc tggtcatcga tgcacgggcc
      301 tcgtccgtcg cggcttcggc gcagacgtca ctgctcttcg cgatggcgcc gtttgcgctt
      361 ttttccgccg tggcgccgca gtttgtcgag agcgcgggga gtgggcgccg gtacggcctt
      421 cgataggccc aagatggctt cgttgccgcg cgctcgctca cgcgccatct cgatgaagcg
      481 ctgttggctg cggcgtggcg tggcgccctc ggacggtcgc tcgcgctcgt acttgccgtt
      541 gtcgcgcagg acccagctct tgcagttgtc ctcgcgcatg gtctggagga tctcggtcac
      601 gacgcgcgtc ttgagatccg gatcgagcac ggggaacatc acctcgacac ggcgccggaa
      661 gttgcgcggc atccagtcgg cgctcgagca atagacctcc tcgttgccgc cgttcttgaa
      721 gtggaagatg cgcgcgtgct cgaggaagcg atcgatgaga gctttgacgc ggatgcgctc
      781 actgatgcca ggtacgcccg ggcgtaggca gcaaatgccg cgcacgagga ggtcgacttc
      841 gacccctgct tgcgaggcgg cgtacagggc ggcgatcacg tccgcgtcga cgagcgagtt
      901 catcttggcg atgatgtgcg cagggcggcc gctgagcgcg tgcgccgtct cacgtgcgat
      961 gagcccgagc accgcttcgt gcaagccgag cggcgcgacg atccagtggt tccagcgcac
     1021 cggcgcgctg tagccggtga gcatgttgaa gaacgcggtc gcgtcttcgc cgtagtcttc
     1081 gcgcgcggta aagagcgaga tgtcttcgta gaggcgcgcc gtttgctggt tgtagttgcc
     1141 ggtcgcgagg tgcacgtacc ggcggagccc gttgcgctcg cggcgaatca ccatgagtac
     1201 cttggcgtgg gtcttgaaac ccaagaggcc gtacatcaca ttcacgccgg agcgctcgag
     1261 cgcgcgcgcc cattggatgt tgctctcctc gtcgaagcgc gccttgagct cgacgagcgc
     1321 ggtgacctgc ttgccgagct ccgccgcgcg ctgcagggct ttgaccaacg gcgactcacc
     1381 gccggtgcgg tacagcgtct gcttgatcgc gagcacgcgc ggatcttccg cggcttgcca
     1441 cagaaagtcg atcacggcct cgaacgattc gtaggggtgg tgcaggagta cgtcgcgctc
     1501 cgcgatcgac tcgaaaaagt cctcggtgtc acgcagcggc ggcacgtact ggggcgtaaa
     1561 cgcctcgtcg cgtaggtcgc ggcgctcgtc gccagacgtg agcacggtca tgtctccgag
     1621 gaacagcggc ccgtcgacga gatagacgtc ggtttttgga ttcaagtcga gctcgcggca
     1681 gagccactga atcgactctt cgggcgcgtt gccactgacc tcgatgcgca cggcgttgcc
     1741 gcgctcgcgt cgccgaagct ccgcctgcaa ggtcagcagc aggtcctcgc cttcttcttc
     1801 gtcgagctcg atgtcgaagt tgcgcgtgac gcggaaggca tactgcccga tcaccttgag
     1861 ctgcgggaag atcatgcgcg tgtgccggaa gatcagatct tcgagtgaga cgaacgcgcg
     1921 ccgcgtgccc tcgaccggga cacggatgaa gcgcggcaac atcgtcggca cctgcaccaa
     1981 cccgaacgat ggctcggtcg agtgcagccg cttctcgaac atgagcccca cgttgatgct
     2041 cttgttgcgc acgtgcggga acgggtgcac gggatcgatc gcgatgggcg tgagcaccgg
     2101 atacacgttc ttgacgaagt gctcgtcgat ggcggcttgc tctttctggt tcagctcgtc
     2161 gggcgacacc accgcgagcc ccgccgcgcg cagctctttc cgcagggtcg agttccagat
     2221 gccgtaggtc tctttgatga gctcgtgcac ccgcgtcgcg atcgacgtca gctgctgctc
     2281 gggcgtctgc ccgtcgggcg gcacctcttc gacctcgccg tagagctggg cttgcagccc
     2341 tgccgcgcgc accatgaaga actcgtccag gttgctcgag aagatcgcga cgaatttgag
     2401 ccgctcgtag agcggaacag cagggtccgc cgcctccgcc agcacacgcg cgttgaactc
     2461 gagccacgag agctcgcggt tgagataaag agcgggatcg tcgagccgcg tcggaactac
     2521 accgacaggt gtttcaacgg gcaagctcag cggagtgcgg cttgcatcga cacgactagg
     2581 ttctgcagac gacatgggac ctgttgtttt ggaggtcggc atcctaacgc gaggctcctg
     2641 tcagacatcg tttcgaagca tcaattggtc gcactagtca caaactcgtc acacgagggg
     2701 gagccgcggg cactcgcccg agtgctgcaa gcttgctgca tgccgaccgc tcgcgtgaag
     2761 catcgcgcgg cttttgcgcg cttcgcgtaa cggtcgacgg gcctgctcgc gtgttcgaaa
     2821 ggcgagtttc ccgctcgatt gctgaacaag tgttcgtttg ggccgaagcg acctccgggt
     2881 gccaaaaatt cgggtggtac cagctcgcat cacgcctggg tcttcaacgg atgcccatcc
     2941 gactacgcgg tgccctcact gaccgaaagg ggccgctccc cgaatcccgg cgctcacgcg
     3001 cgtgctccaa agcttgcgcg tgcctccaaa ggttgcgcga gcctccgcag attgcgcgag
     3061 cctccacaga ttgcgcgagc ctccgcagat tgcgcgagcc tccacagatt gcgcgagcc
//
LOCUS       DASTKS010000019         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_3256,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000019 DASTKS010000000
VERSION     DASTKS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3256"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..82)
                     /locus_tag="VFQ35_00375"
     tRNA            complement(12..82)
                     /locus_tag="VFQ35_00375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Thr,seq:cgt)
     gene            complement(123..194)
                     /locus_tag="VFQ35_00380"
     tRNA            complement(123..194)
                     /locus_tag="VFQ35_00380"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Asn,seq:gtt)
     gene            complement(330..403)
                     /locus_tag="VFQ35_00385"
     tRNA            complement(330..403)
                     /locus_tag="VFQ35_00385"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Gln,seq:ctg)
     gene            1638..2132
                     /locus_tag="VFQ35_00390"
     CDS             1638..2132
                     /locus_tag="VFQ35_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9929105.1"
                     /translation="MSCPIALSLEEVGEAWSLLILRDVFMGVRRFHDLELRLGISAST
                     LTRRLAELCRKGLLHATLYEQKPKRYEYTLTDKGEDLLEVLLALGAWGNRWLTQVIVP
                     VDANTGARVDPVLVDRVTGRRLSAGQVALAAGRDASSKMREFLKPPRKLGRCAPTLVL
                     EASS"
     gene            2129..>3060
                     /locus_tag="VFQ35_00395"
     CDS             2129..>3060
                     /locus_tag="VFQ35_00395"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9929106.1"
                     /translation="MRRVVVTGLGLVTPLGVGVAHVWNELLAGRSGIRKITSFETSDL
                     SARIAGQVPRGASAGELDLDALFDAKERRRLDDFILYALASATEALADSGFTPSNDAQ
                     RERAGVLFGSGIGGLPAIAETSVKLEQQGPKRVSPFFIPSALINEASGLVAIRFGLRG
                     PCHAVVTACATGANAIGDAARMIQLGDADVMVCGGTEAATCRLSLAGFGAMRALSTAF
                     NDDPEHACRPWDEARDGFVLAEGAGSLVLEELEFAERRGARIYGEVTGYGIGGDGYHL
                     SAPDPSGSGAVRAMTSALARAGIAASEIGYVNAHA"
BASE COUNT          580 a          961 c          997 g          522 t
ORIGIN      
        1 gccagcgata gagctcccgc gcggaatcga accgcgtccg gcgcattacg aatgcgctgc
       61 tcgaccatcg agctacgaga gccacgaacg cgcggcgacg gtgtgagacc gatgccgcgc
      121 agctccgaag gctggaatcg aaccaacatc tcctcgctta acaggcgagc gcacgaccat
      181 tgtgctactt cggaatgcta tccggaggca gcgccgcttg acagcgccgc ctccatcaca
      241 cacacgcggg ggaccgcacg ccgcgctctt cgcgccgcgc cgtcgagccg cgttcgaatt
      301 gtcagagatc acggagcaca cgactccgat ggttccggga gttggagtcg aaccaaccca
      361 gcgagcttca gagactcgcg tcctaccact agacgacccc ggaatatgcg aatgaactaa
      421 cgagaacctc agtcgcgata cgacttcaga atgaatgacc acgaaccaag aactagacac
      481 acaactcgcc ccaacatcga ttgctttgaa tctcgaacct tgaattgcca atcagcagac
      541 cacgatgtcc gaccccgcgc acacgagtac tttggatgac tccgcactca gcccctgcga
      601 ggcacgaagc gccgtgagca cacaacgagc gcctcggtga cgtgccctct gcgcgacgac
      661 tagcccaaaa cgaaaaggcc gccgaggttt ccctgggcgg cctttcgcga tgaatcgcga
      721 gcagaatcgc ccacttacat agtattatcc gccagcaagc aacctaggag gcgcgccgcc
      781 gcgcactcgc atccctgccc ggacacgggc ccgcgccggc tgccgaatcg gcgcgagctc
      841 gggggttgca acatgacgaa cgtgaacatg acgagtgttt gaacaatata ctcgcgaccg
      901 agctcgcgca agaactaaat cgctttgatc ttcattttag atctcgagtc gattgcgcaa
      961 acgattgcgc aacgcgtggt gcgttgttcc cggtcgcgct tgcaccgtat caattgggag
     1021 taagtgccgc cttttcgtca gtgcgaggat gaggggcaga ggcaggctcg aagagcgatc
     1081 gccggttttg acgtatgcac gactcgagtc gactcgagtc gcgtgtgggc accgcccagc
     1141 gcaaaggcgt ttagatggtg gagctcgagc ccggtgcgtc tctcgacagc gttgccgcga
     1201 gccgtcgatg tacgtgcagc gcccctcgtc gcgttcgaag acggcgcgcc ggatggcggc
     1261 agggacatgt cggccgcgcc agcgctgacg gatccccgca ttcatcaagg cgaaccgcac
     1321 gccgaggcag cgacgagtga ccgcaggaag tttgagaagg cggctcggag aggccctacc
     1381 agcacttgtt tcattgagct ccgagcaaca atgagtttgg cgagaaagaa ggacgagaga
     1441 ggcgggcgcc cgagaagaga gcagcggagc ccgcccatgc gtggttcgga gcgaagtgcg
     1501 cgcccggaca aatgcgctcg ttgcgtcgtc gatcgcgccc gtccctacca atccgcaagc
     1561 cgcctctggg ctggtcactt gcatcatgaa agtcgatatg ccaacgtctc tgcgtgcaaa
     1621 ggaaatcgtt ttcgcgaatg tcgtgcccca tcgcgctctc gttggaagag gtcggcgagg
     1681 cgtggagcct cttgatcctg cgcgacgtgt tcatgggagt gcgtcgcttt cacgatttgg
     1741 agctgcggct cggcatcagc gcgagcacat tgacgcgccg gctcgcggag ctctgccgca
     1801 aaggcttgct gcacgcgacg ctctacgagc agaagcccaa gcgctacgaa tacacgctca
     1861 ccgacaaggg cgaggatttg ctcgaggtgc tgctcgcgct cggcgcctgg ggaaaccgct
     1921 ggttgacgca ggtgatcgtc ccggtcgacg ccaacaccgg tgcgcgcgtc gatccggtgt
     1981 tggtcgaccg cgtgacggga aggcgcttga gcgctgggca ggtggcgctc gcggccgggc
     2041 gcgatgcatc cagcaagatg cgagagttcc tgaagcctcc gcggaagctc gggcggtgcg
     2101 cgccgactct cgttctggag gcgtcgtcgt gaggcgcgtg gtggtcaccg ggctcggtct
     2161 cgtgacgccg ctcggtgtcg gtgtcgcgca cgtgtggaac gagctcttgg ctgggcgctc
     2221 cgggattcgc aaaattacga gcttcgaaac gagtgacctc tcggcgcgca tcgccggcca
     2281 agtcccccgc ggcgcgtcag cgggagagct cgacctcgat gcgttgttcg atgcaaagga
     2341 gcgacgacgg ctggacgact tcatccttta cgcgctcgct tccgccaccg aggcgctcgc
     2401 cgactcgggg ttcacgccga gcaacgacgc gcagcgcgaa cgcgcgggag tgctcttcgg
     2461 ctcgggcatc ggcgggcttc ctgcaatcgc cgagacgtct gtgaagctcg agcagcaagg
     2521 gccaaagcga gtgtcgccgt ttttcatccc gagcgccctc atcaacgaag cgtcagggtt
     2581 ggtcgcgatt cggtttgggc tgcgcggccc ctgccatgcg gtggtcacgg cgtgtgcgac
     2641 cggagccaac gcgatcggtg acgcggctcg catgattcag ctcggcgacg cggacgtcat
     2701 ggtatgcggc gggaccgagg ccgccacgtg ccgtctatcc ctcgcggggt tcggcgcgat
     2761 gcgggcgctc tctacggcgt tcaacgacga cccagagcac gcgtgtcgtc cgtgggacga
     2821 agcgcgcgac ggcttcgtcc tcgcagaagg cgcagggagc ctcgtgctag aggagctcga
     2881 attcgccgag cgccgtggcg cgcgcattta cggcgaggtt accggttatg gcatcggtgg
     2941 cgatggctat cacctgagtg ccccggatcc gagcgggagc ggcgccgttc gtgccatgac
     3001 atcggccctc gcgcgcgctg gcatcgccgc cagcgagatc ggctacgtga acgcccacgc
//
LOCUS       DASTKS010000020         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_226150, whole genome shotgun sequence.
ACCESSION   DASTKS010000020 DASTKS010000000
VERSION     DASTKS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226150"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFQ35_00400"
     CDS             complement(<1..483)
                     /locus_tag="VFQ35_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HET9929107.1"
                     /translation="MSRPSGDGGSAPSTAGSTSDPALDCASPKPGRAPLRRLTTREFN
                     NTVRDLLGDTTNPGDLLPPQVDSKDNWFGNDADFQSVPDTLVEKYQSIAESIAARATA
                     STTALGRLHSCAGKAVAAADEESCARSIAESIAPRAYRRATAKGDIDDLVALFRSVRA
                     L"
     gene            complement(955..1932)
                     /locus_tag="VFQ35_00405"
     CDS             complement(955..1932)
                     /locus_tag="VFQ35_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929108.1"
                     /translation="MTLRTLVLTTTLALTACGETLDAGSSRPHGSLPVDERNPIILTN
                     DNVGENWQGEYALLLANGGGPKLAGIVVNTSGPWPDIEANVEGWRALVTAARASGLDA
                     PDPLASVGATLEKPASGLIEDTVPNRSEGARFIVDTSLALSLPYRPVVITTGGRLTDI
                     ADAYLMDPSVAERIVVVSSLGSISESGAVMANPNGEMDSWADVIVTSRLRYVQVSAFY
                     DQLTDVPASRVSELPTNALGAWIADKQPHIWDLPEASDQVAIAAVGIPNFAATVVRAS
                     AAPSTAKPRQGPDLTTDPNGPIWLVREVKGSLASASFWQRLSELGAVSN"
BASE COUNT          423 a          784 c          835 g          443 t
ORIGIN      
        1 gagcgctcga accgaacgaa acagcgcgac cagatcgtcg atgtctcctt tcgcggtcgc
       61 gcgccgatag gcccgtggcg cgatgctctc ggcgatcgag cgcgcgcagc tctcttcgtc
      121 ggcggcggcc acggccttgc ccgcgcacga gtgcaggcgc ccgagcgcgg tcgtgctcgc
      181 ggtggctcgc gccgcgatgc tctcggcgat gctttggtac ttttcgacga gggtgtcggg
      241 gaccgattga aagtcggcgt cgttcccgaa ccaattgtct ttgctgtcga cttgcggggg
      301 aagcaggtcg cccgggttcg tcgtgtcgcc cagcaagtcg cgcacggtgt tgttgaattc
      361 gcgcgtggtc agccgacgca gcggagcgcg tccgggtttg ggcgaagcac aatcgagcgc
      421 cggatcgctc gtgcttcctg cggtggaagg agccgagccg ccgtcgccgg aaggtcgtga
      481 catcgtcgac ccccccgccc cggtgggccc agcgcccccg ctcgagcctt tgccgttggt
      541 gggcgttcct gcagctccgg aactaccggc gggggttgtg aaagctccgc cgctcgcggg
      601 cgagctgcca gcgtcgatga tctctccggt gcatccacaa aggacgagag cggtcaggca
      661 tctaccgaac cgaaggcgaa ctgagccgaa gcgagcgccg tcgacgcggc gtgcgcgata
      721 gtcgtgtcgc atggtgcgtg ctcagcgtgg aactgtcgcg acgctttcga tgttgtgaat
      781 caaactcacg catcgcgcgt ttttcatcag gagagccgcg gacagcgtaa agattcagcg
      841 tggcaccaaa gtcgcggtgt ctcggggact ctgctcgatg ctgcgtcggc gcgcgccctc
      901 cgaggcgcgc cgggagcgtc ctcgaagcga ttcgaaagga tcaattcgaa aagatcagtt
      961 cgaaacggct ccgagctcgg agagccgttg ccagaagctc gccgaggcca gcgagccttt
     1021 gacctctctc accaaccaaa tgggtccgtt cggatctgtc gtcagatccg gaccctgacg
     1081 gggctttgcg gtcgaaggtg cggctgatgc tctcactacg gtcgcagcga agtttgggat
     1141 gccgaccgca gcgatcgcga cctggtccga tgcctcgggt aaatcccaga tgtgcggttg
     1201 cttgtccgcg atccacgcgc caagtgcgtt ggtcgggagc tccgagacgc gggaagccgg
     1261 cacgtccgtt agctggtcgt aaaatgcgct cacctgtacg taacgtaggc ggctcgtcac
     1321 gatgacgtcg gcccatgaat ccatctctcc attcggattc gccatcaccg cgcccgattc
     1381 ggaaatgctg ccaagcgacg acacaacgac gattcgctcg gcgacgctgg gatccatcag
     1441 ataagcatcg gcgatgtcgg tgaggcggcc tccggtcgtg atcacgaccg gccgatacgg
     1501 aaggctgagc gcgagagacg tgtcgacgat gaagcgcgcg ccctcggagc gattgggcac
     1561 cgtatcttcg atgaggccac tcgccggctt ttcgagggta gccccgacgc tcgcgagcgg
     1621 atctggcgca tcgagcccgc tcgcgcgcgc cgccgtcacg agcgcgcgcc agccctcgac
     1681 gttcgcctcg atgtcgggcc agggcccgct cgtgttgacg acgattccag cgagctttgg
     1741 gccgcctccg ttcgcgagca acaaggcgta ctctccttgc caattctcgc cgacgttgtc
     1801 gttcgtgagg atgatcgggt tgcgttcgtc gaccggcagc gagccgtgtg gtcgcgacga
     1861 gccagcgtcc aaggtttcac cgcacgcagt cagcgcgaga gtcgtcgtca gcaccagcgt
     1921 tcgcagcgtc acgagcggga gtgtctcacg aatcgagctc ggacgcacag cattgccgct
     1981 cagcgcgctg cgcgttcggc cgcgaccgtc gcgcgcgcgc gcagcaattg gcgcgcatga
     2041 acggagcgcg actgacgcgg tatgaggcgt cccgcctcga gcagcgccag aaggaacggt
     2101 ggcacaaggt gagagcttac gctccgacga cgggcgctcg agtgcaccag gctgagatta
     2161 gcggctgcca tccaggtcga cttttattgt cgcgaagaag ggtggtgttc atcaaaagca
     2221 gcatcgatcg tcgaaaagca cttttcgtca caaccgagcc accttcgctg cagtagagga
     2281 agatggctgt cggacgaccc tccgtgtgca cccagaacgc gagcccctcc cgccgtgggc
     2341 gtggctgtct acgcgccaca acggcgggtc gacgcatgct cgttgcggtg ccgctgacgt
     2401 tggccgcgct cggctgttca gcgcagaaac aggacgaatc aggcggagcc ggcggtggca
     2461 atggcggcgc gctcggcggc tctcc
//
LOCUS       DASTKS010000021         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_226272, whole genome shotgun sequence.
ACCESSION   DASTKS010000021 DASTKS010000000
VERSION     DASTKS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226272"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFQ35_00410"
     CDS             <1..481
                     /locus_tag="VFQ35_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9929109.1"
                     /translation="GEVGIETVMPPRDPNSTTRARFAAHTSNPMCKSCHATIDPLGFS
                     FEGFDAAGRRRTREQGKPVVTAGTLNIGGKALNFADSAELSAALSGDPDSRRCFARHA
                     LRYLTGRRATATEDWFEQVVDGLPSERRDSLLEWVVAWVRSPEFIRRRANAESPSKP"
     gene            478..1878
                     /locus_tag="VFQ35_00415"
     CDS             478..1878
                     /locus_tag="VFQ35_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9929110.1"
                     /translation="MTRLPTKTWSRRATCGRRELLFALGAALLPRRVWADMAPQAPTP
                     APSTAKSENAPAKRFVGVYMPHGRAQELFLPGPDFRLDYEDAILQPFDDAARYGRSLR
                     GDLLVVDGLDLAAGFLTGNVGHDAPRVLFTGAGANGQNASLDQFLAVDQRLGDATSVP
                     SIVLAVGNDDRGPTSSVSYGRGGVPLPKLIHPAATFDALFGAALTGSPEVAALRRERS
                     MSALDFLGEDLARLRRVAPASSRQSLEHHQTALRDIERRLSQPATECTPPPRVQRDAF
                     PAYRAYGGGERSFDAITDLQIELVVRAMACDVTRFATLMLNDLSRTGLDPMLPNDVHQ
                     DVAHRYVARDEHKPGNPASWKALARQNRYTCSKVALLAAKLHEQHLLADTLVLASSDM
                     GDPGRHSSRSVPTLLLGAGVRGGRVLRVGDEHRVTAQNLKPNNRLLAHVCQRFGVMVT
                     RFGDSTPEISEGVLEV"
     gene            complement(1897..2394)
                     /locus_tag="VFQ35_00420"
     CDS             complement(1897..2394)
                     /locus_tag="VFQ35_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929111.1"
                     /translation="MLRYFVFVCGALVAACSSGDSGSNGGGDSTGKSEAQWPGDPCTL
                     LTSADFAGLPGSPTISKSEAHDLMSQQFSPTCYFYVKGDTVSDTLHLAVDPEADFDLQ
                     QTLFKAQPVSGIGKAAWHGNNGGVGNLTVGVLVDGYSFRIDSTSEYEYPELDVLAKLI
                     APRIK"
     gene            2661..>3702
                     /locus_tag="VFQ35_00425"
     CDS             2661..>3702
                     /locus_tag="VFQ35_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929112.1"
                     /translation="MTTAGDSPFNRRRFLLGGGVFGLGLLGASCSSDSSSGGAAPTPP
                     SEDVWSVLKTLRELARSSPDHLAARFAEAIAAKSPENLVRLVQERVRVLPTDGADVLW
                     SCRWGASGALRAAAGTPRERMDLLAAALKALGAVTRVVSASLPASVTADGLYRDASPV
                     FALDAAKLASLRGSKLAESLAKLPTSGTEPDPTATAEKLLRAIPVAQATAKHRAMQTL
                     PSKLPVVEFTLAGVTRWAYAIADLGIVTATPEGLTGELTEADIPSVSLRVLGALGDLP
                     SAAGSSSLTELVSGTWRADRVAGRKVVLTFPPPGDPRSGFGQKLESVPIRVPSLVLTE
                     PDPPGPGQAEPFS"
BASE COUNT          618 a         1269 c         1175 g          640 t
ORIGIN      
        1 gggcgaagtc ggcatcgaga ccgtgatgcc cccgcgggat cccaacagca cgacgcgcgc
       61 gcgcttcgcg gcccacacca gtaacccgat gtgcaagtct tgccacgcga cgatagatcc
      121 cctcggcttc agcttcgaag gcttcgatgc agctggccgc cgccgcaccc gtgagcaagg
      181 aaaaccggtg gtgaccgccg gaacgttgaa cataggcggc aaagcgctga acttcgccga
      241 tagcgcggaa ctctccgcgg cgttgtcggg agaccccgac tcgcgccgtt gcttcgcccg
      301 gcacgcgctg cgctatctaa cggggcgccg cgccaccgca accgaggact ggttcgagca
      361 ggtcgtggat ggccttccca gcgaacggcg cgacagtttg ctggaatggg tggtcgcctg
      421 ggtccgaagc cccgagttca ttcgaagacg ggcgaacgca gaaagtccct cgaagccatg
      481 acgagactcc ctactaagac ttggtctcgc cgcgccacgt gtggacgccg agagctcctg
      541 tttgcgctcg gcgctgcgct gctgccgcga agggtttggg cggacatggc tccgcaagcg
      601 ccgacgccgg ctccgagcac tgcgaaatcc gagaacgcac ctgccaagcg cttcgtcggt
      661 gtctacatgc cgcacgggcg cgcgcaggag ctgttcctcc ccgggcctga ttttcgcctc
      721 gactacgaag acgcgatcct ccaacccttc gacgatgccg cgcgctacgg gcgctcgttg
      781 cggggagact tgctcgtggt cgacgggctc gatctcgcgg ctggttttct caccggcaac
      841 gtcggtcacg acgcgccgcg cgtgctcttc acgggcgcag gcgcgaatgg tcaaaacgcg
      901 tcgctcgacc agttcttggc cgtcgaccag cggctcggcg atgcgacctc cgtcccgagc
      961 atcgtgctcg cggtcggcaa cgacgatcgc ggtcccacga gctcggtcag ctatggtcgc
     1021 ggcggcgttc cgctcccgaa attgattcat cccgccgcga cgttcgacgc gctgttcggg
     1081 gctgcgctca ctggttcacc cgaagtcgcc gcgctgcgcc gcgaacgctc gatgagcgcg
     1141 ctcgacttcc ttggggagga cctcgcccgt cttcgtcgag tcgcgcccgc gagctcacgc
     1201 cagagcctcg aacaccacca gaccgcgctg cgcgatatcg agcggcgcct ctcgcagccc
     1261 gccaccgagt gcacgccgcc tccccgtgtc cagcgcgatg ccttccccgc ctatcgcgcc
     1321 tacggtgggg gcgagcgctc gttcgacgcc atcacggatc tgcaaatcga gctggtggtg
     1381 cgcgccatgg cctgcgacgt tacgcgcttt gcgactctca tgctcaacga cctgtcgcgc
     1441 accgggctcg accccatgct cccgaacgac gtgcaccaag acgtggcgca ccgctacgtc
     1501 gcgcgcgacg agcacaagcc cggcaacccc gcgagctgga aagccctcgc gcgtcaaaac
     1561 cgctacacgt gctccaaggt cgcgctgctc gcggcgaagc ttcacgaaca gcacctgctc
     1621 gcggacacgc tggtgctcgc cagctcggac atgggagacc cgggccgcca cagctctcgc
     1681 tcggtcccga ctttactgct cggcgccggg gttcgcggcg ggcgcgtact ccgcgtcggg
     1741 gacgagcacc gcgttacggc ccagaacctc aagccgaaca atcgtctgct cgctcacgtt
     1801 tgccagcgct tcggcgtgat ggtgacgcgc ttcggagatt cgactccgga aatcagcgaa
     1861 ggcgtcctcg aggtgtgatt tcgaaagtgc tccgcctcat ttgatgcgcg gtgcgatcag
     1921 cttggcgagc acgtcgagct ccgggtactc gtactcgctc gtcgaatcga tgcgaaagct
     1981 gtagccgtcc acgagcactc ccactgtaag gttaccaacg ccgccgttgt tgccgtgcca
     2041 cgcggcctta ccgatgccgc tcaccggctg agccttgaaa agtgtctgct gcaagtcgaa
     2101 gtccgcctcg ggatcgacgg ccaagtgcaa cgtgtccgag acggtgtctc ccttcacgta
     2161 gaaatagcac gtgggagaga actgctgact catgaggtcg tgcgcctcgc tcttcgagat
     2221 ggttggcgag cccgggaggc ctgcgaagtc ggcgctggtg agcagtgtgc agggatcgcc
     2281 aggccattga gcctcgcttt tgccagtgct gtctccgccc ccgttgctcc cgctgtcgcc
     2341 gctcgagcaa gcggccacga gagctccgca cacgaacacg aagtatcgca acattgccat
     2401 cacgatagcg ccggcactca cttccaacta caatttttgc gaagacgcca tacgcgagta
     2461 ttcgcgctcg cagagctttg gtactttggt ctgccgagac tcggggcgcc ggccccaagc
     2521 gcggccacat cctgaatttt cgctgaaaac gagggaattc cctcagggcc ttggacccca
     2581 agcggctcgg gcagctggcg gcgagatgtg atgaatgacg ggctcggaca gctcaactct
     2641 catccgacgg ggtaattcgc gtgacgactg caggtgattc tccgttcaac cgccgtcggt
     2701 tcttgctcgg cggcggcgtt ttcgggcttg ggctcctcgg cgcgagctgt tcttcggact
     2761 cttcgtcagg aggagcagcg ccgacccctc ccagcgaaga cgtctggtcc gtcctgaaga
     2821 cgctgcgtga actcgctcgt tcgagcccag accacctcgc cgcgcgcttt gccgaagcga
     2881 tagccgcgaa gagcccggag aacctcgtgc gcttggtgca agagcgcgtg cgagtgttgc
     2941 ccaccgacgg agccgacgtc ctctggtcct gccgctgggg cgcttccggc gcgctgcgcg
     3001 cagcggctgg cacgccgcga gaacgcatgg acctcttggc ggcggcgctg aaggcattag
     3061 gcgccgtcac gcgcgtcgtc agcgcgagcc tccccgcatc ggtcaccgcc gacggactgt
     3121 atcgcgacgc ttcgccggtg ttcgcgttgg acgcggccaa gctcgcgtcg ctgcgcggga
     3181 gcaagctcgc agagtcactc gcaaagctgc ccacgagcgg caccgagccg gacccaaccg
     3241 ccacggcaga aaagctcctg cgggcgattc cggtggcgca agcgacggcc aagcatcgcg
     3301 cgatgcaaac gctgcctagc aagctccccg tcgtggaatt tacgctcgcc ggtgtcactc
     3361 gttgggccta cgcgatcgcc gatctgggca tcgttacggc gacgccggag ggactcacgg
     3421 gcgagctgac cgaagccgac attccgagcg tctcactgcg ggtactcggc gctttggggg
     3481 atttaccgag cgcggccggg agctcgagcc tgactgagct cgtgagcggt acttggcgag
     3541 ccgatcgcgt cgccgggcgc aaggtggttc tcacgtttcc cccacctgga gatccacgaa
     3601 gcggcttcgg ccaaaagctg gagtcagtgc caatccgagt gcccagcttg gtgctcaccg
     3661 aaccggatcc gccaggcccc ggccaagccg agccgttctc gc
//
LOCUS       DASTKS010000022         6717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_3455,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000022 DASTKS010000000
VERSION     DASTKS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFQ35_00430"
     CDS             <1..759
                     /locus_tag="VFQ35_00430"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9929113.1"
                     /translation="RVFYLPEPFRLVIDVMKEGLAPPGTSPQKPRVRRVVLDPGHGGH
                     DPGAIGPSGLREKDVALDIAHRAAPLIARELGISTLLTRDADEFVALDERTARANAFH
                     ADLFVSIHCNASEDIAARGVMTFVLDAGREELSARIAARENAASSEAAVELANSFSRV
                     TDPATRAASARFAELLQRSAMASLTQRYPEVQNGGVKRAGFYVLAGARMPASLFETSF
                     ISNPQGEARLNSGDFRQRMADAIVNAIRAYRDGL"
     gene            1022..1384
                     /locus_tag="VFQ35_00435"
     CDS             1022..1384
                     /locus_tag="VFQ35_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET9929114.1"
                     /translation="MTKVQLKKFKEMLEAKRDEIIKRAQQTLDEDMALDANDLPDEMD
                     LASSEYLQSFTFRLRGREKVFLDKIQKALEKIENGSFGVCDECGEKISVKRLEARPET
                     TLCIRCKEDQERVEKDFT"
     gene            complement(1504..4413)
                     /gene="uvrA"
                     /locus_tag="VFQ35_00440"
     CDS             complement(1504..4413)
                     /gene="uvrA"
                     /locus_tag="VFQ35_00440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9929115.1"
                     /translation="MQEVLVVRGASQHNLKGVSCELPRNRLVVITGPSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLDQLPKPRVESIEGLSPAIAIEQKGLGKSPRSTVGTV
                     TEIADYLRLMYARVGVPHCPKSGKVLRAYTVQEIVDAIADRGDGARIVLLAPVMRRFQ
                     GDVREELERLRREGFVRARLNGQQVDLGDDFTVQPEAEYDLDVVVDRIVVREGIKGRI
                     TDSVELALKLGQGTVLIDQTDGTEPVVMSERLVSWEYGITLPPLEPRLFSFNSPHGAC
                     PVCDGLGVRSAIDVNRVVPDPTRPLREGAIAAFGRRGSVATATEVARVVDALGVDPDV
                     PFQDLPEETRNAILFGTTAHPVKRRGRPREYDGVVPRLARMLETNELPDGEDEPEDGA
                     IGPEDLGRFVITQTCEACDGVRLRPEALSVRIGGKNIGELCRLPLRALKDFLGEYASS
                     SELLGRDATIALPLVRAVSERLGFLIEVGLDYLTLDRTAHTLSGGEGQRIRLATQIGA
                     SLVGVLYVLDEPSIGLHARDNERLLSALRRLVDKGNSVIVVEHDRDAILAADYVLDMG
                     PRAGAHGGEIVAQGTPQELAENPESVTGPYLSGKKRLPIPSSRKAPSGSQLRVVGARA
                     HNLREVTLEIPLGLITAVTGVSGSGKSSLVVDTLLSAVRAELYGASGWVGPCDRIEGL
                     EHIDKVISIDQAPIGRTPRSNPATYTGIFTHLRELYAGLPDARARGYKPGRFSFNVKG
                     GRCEACQGDGVLRIEMHFLPDVYVTCDTCAGRRYNRETLEVRYRGLSIADALDLTVDE
                     ADELFESIPRMRQRLSALRQVGLGYIQLGQPATTLSGGEAQRVKLATELARKATGRTL
                     YVLDEPTTGLHFQDIELLTHALFGLRDAGNSVLIIEHNIDLVACADWVVDMGPEGGEH
                     GGLIVAAGTPEEIAATEASYTGRYLKQVLETEAGAQASPRPTPTAERRSAGRRRR"
     gene            4895..4968
                     /locus_tag="VFQ35_00445"
     tRNA            4895..4968
                     /locus_tag="VFQ35_00445"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4929..4931,aa:Ile,seq:cat)
     gene            5108..5854
                     /locus_tag="VFQ35_00450"
     CDS             5108..5854
                     /locus_tag="VFQ35_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9929116.1"
                     /translation="MTIQAQIEVLEALAALDAELKELNDALSLEREGLRSKQSQLSSL
                     DERFERSRKSVEDMERLRNDLLGELRQMGTQVERSREKLSRCRTEREANAAQRELEEL
                     RKLYRDREVESEKLESLISQAREESGQLSTDRQKLSADLGASEGDVVGRLETSERSLS
                     EKLSARADLVKRVDPALYRRYELVRKRKGSAIAHTSTGTCSACHMAVQPMLFQKLLRS
                     DEFGQCPHCNRILYYRPAKAGESEDSSESA"
     gene            5904..>6717
                     /locus_tag="VFQ35_00455"
     CDS             5904..>6717
                     /locus_tag="VFQ35_00455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929117.1"
                     /translation="MKACAQCGRLYPDDAGFCPVDGAALGRPTSVPVPVDATDKRVGQ
                     LVCQRYQIRRVVADGGMGRVYEALDLRVKRNVALKILHAEVADDEVAVERFKREFEVS
                     KQLPHDYIVEVLDFQPTGDNTYALAMEYLYGEELRATLKREVVISPARLVRMLSQIAI
                     GLDEAHARQFVHRDLKPDNLFLGQTSDGDIVKILDFGSVKDKGESAKKLTVLGTTIGS
                     PFYMAPEQAQGLDTLDHRADVWALAAITYECATGQVPFQGNNGPSILLEILTK"
BASE COUNT         1144 a         2169 c         2230 g         1174 t
ORIGIN      
        1 cgcgtattct atttgccgga gccgtttcgc ttggtcatcg acgtgatgaa agagggcctc
       61 gccccgccgg gaacctctcc acagaagccg cgggtgcgtc gagtcgtgct cgacccgggc
      121 cacggcgggc acgatccagg cgcgatcgga ccgagcggac tgcgcgaaaa agacgtcgcg
      181 ctcgacatcg ctcatcgcgc cgcaccgctc atcgcgcggg agctcggcat ctcgacgctg
      241 ctcacgcgcg acgcggacga attcgttgcc ctcgacgaga gaaccgcgcg cgcgaacgcc
      301 ttccacgccg atctcttcgt ttcgatccac tgcaacgcga gcgaggacat cgcggcgcgg
      361 ggggtcatga ccttcgtgct cgacgctggg agagaagagc tctcggcacg cattgctgcg
      421 cgagaaaacg cagcctcctc cgaagcggcg gtcgaactcg cgaattcgtt ttctcgagtg
      481 accgatccag cgacacgcgc ggcgtcggct cgcttcgccg agctcctgca acgctcggcc
      541 atggcgtcgc tgacccagcg ctacccggaa gttcagaacg gaggcgtgaa gcgcgcgggc
      601 ttctacgtgc tcgcgggtgc gcgcatgccg gcgtcgctgt tcgagacttc tttcatctcg
      661 aatccgcagg gtgaggcgcg cttgaacagc ggcgatttca ggcagcgcat ggctgacgcg
      721 atcgtcaacg cgattcgcgc ttatcgcgac ggattgtgac tgtcgtttac gaacgtcggg
      781 ggcggacggc gcgcgaagct ctcgtcggga gtggccctgg gtcgattccg gtaaagcggt
      841 cgcgttcgcg ggaatgcaga agagagcgcc ggacccctgg ccgaagccgc cgcggggagt
      901 tcgctttttg gtcgactcga cgcacgggcg gcattgacgg agcggtactc cgttcctatt
      961 ttggtgggcc actctgaagt cgccgggcag tccggccact ttcagcctcc ccgagccgcc
     1021 gatgacgaaa gttcagctca agaaattcaa agagatgctc gaggcaaagc gcgacgaaat
     1081 catcaagcgc gcgcagcaga cgctcgacga ggacatggcg ctcgacgcca acgacctgcc
     1141 ggacgagatg gacctcgcgt cgagcgagta cctgcagtcg ttcaccttcc gcctgcgggg
     1201 acgtgagaag gtcttcctcg acaagatcca gaaggctctc gagaagatcg agaacgggag
     1261 cttcggtgta tgcgacgagt gcggagagaa aatctccgtc aagcgcctcg aggctcgacc
     1321 cgaaacgacg ctttgtattc gttgcaagga agaccaggag cgggtcgaaa aagacttcac
     1381 ctgagcagtc cctggtgcgg ccggttcgtc ctattcgagc cggccgcgcg atgggaaaag
     1441 cattagcgcc ggcagagtcc ggcgcttcgt cgtttctagt gcccggcgcg ccgctcgtcg
     1501 tgctcagcgc cgacgccgcc cggcgcttcg cctttcggcg gtcggcgtcg ggcgaggtga
     1561 agcttgtgct ccggcttccg tctcgagcac ctgcttcaag taccttccgg tgtagctcgc
     1621 ttcggtagcc gcgatctctt cgggggtgcc ggcggccacg atgagcccgc catgctcgcc
     1681 gccttccggg cccatgtcga cgacccaatc ggcgcacgcc acgaggtcga tattgtgctc
     1741 gatgatgagc accgagttgc ccgcgtcgcg caacccgaag agcgcatgcg tcagtagttc
     1801 gatgtcctgg aaatggagac cggtcgtggg ctcgtcgagc acgtacaagg tacggccggt
     1861 tgccttgcgc gcgagctcgg tcgcaagctt cacgcgttgc gcttcgccgc cggacagcgt
     1921 cgtcgccggt tggccgagct gaatgtatcc gagccccacc tggcgcaggg ccgagagcct
     1981 ctggcgcatg cgcgggatgc tctcgaagag ctcgtccgct tcgtcgacgg tcaagtcgag
     2041 cgcgtcggcg atcgaaagac cgcggtagcg cacctcgagc gtctcgcggt tgtaacggcg
     2101 gccggcgcac gtgtcgcacg tgacgtagac gtccggcaga aagtgcattt cgatgcgcaa
     2161 gacgccgtcg ccttggcacg cctcgcagcg cccgcccttc acgttgaacg agaagcgacc
     2221 cggcttgtat ccgcgggcgc gcgcgtccgg gagcccggcg tagagctcgc gtaggtgggt
     2281 gaagatacca gtgtacgtcg ccgggttcga ccgcggcgtg cggccgatgg gcgcttggtc
     2341 gatactgatg accttatcga tgtgctcgag cccttcgatg cgatcgcagg gaccgaccca
     2401 gccgctcgcg ccatagagct cggcgcgcac ggcgctgagc agggtgtcga cgaccaggct
     2461 gctcttgccg gagccgctca cgccggttac ggcggtgatg agcccgagcg gaatctcgag
     2521 cgttacttcg cgtaggttgt gagcgcgcgc gccgacgacg cgcagctggc ttccgctcgg
     2581 cgctttgcgc gaactcggga tgggcaggcg ttttttgccg gagaggtagg gcccggtcac
     2641 gctctcgggg ttctccgcaa gctcctgtgg cgtgccctgg gcgacgattt cgcctccgtg
     2701 agcgcccgct cgcggcccca tgtcgagaac gtagtcggcg gccaggatcg cgtcgcgatc
     2761 gtgctcgacg acgatgacgc tgttgccctt gtcgacgagc cggcgcaacg ccgacagcag
     2821 tcgctcgttg tcgcgcgcgt gcaaaccaat gctcggctcg tcgagcacgt aaagcacccc
     2881 gacgagcgac gcgccgatct gcgtcgcgag ccggatacgc tgcccttcgc cgcccgacag
     2941 cgtgtgagcc gtgcgatcca gcgtcaggta gtcgagcccg acttcgatca agaaaccaag
     3001 gcgctcgctc accgcgcgca cgagcggcag cgcgatcgtc gcgtcgcgcc ccaagagctc
     3061 gctgctcgac gcgtattcgc cgaggaaatc tttcaaagcg cgcagcggca agcggcagag
     3121 ctcgccgatg ttcttgccac cgatgcgcac cgagagcgcc tcgggtcgga gccgaacgcc
     3181 gtcgcaggcc tcgcaggtct gcgtgatgac gaagcgcccg agatcttctg ggccaatggc
     3241 gccgtcttcc ggttcgtcct cgccgtcggg cagctcgttg gtctcgagca tgcgggcgag
     3301 gcgaggcacg acgccgtcgt attcgcgcgg gcggccccgg cgcttcaccg gatgcgcggt
     3361 ggtaccgaac aagatcgcgt tgcgcgtctc ctccggtaga tcctggaagg gtacgtccgg
     3421 atcgacgccg agcgcatcga ccacgcgcgc gacctcggtc gccgttgcca ccgagccgcg
     3481 gcgcccgaac gcggcgatgg ctccttcgcg gagcgggcgc gttgggtcgg gcacgacgcg
     3541 attgacgtcg atcgccgagc ggaccccgag tccgtcgcaa acggggcaag cgccgtgagg
     3601 cgagttgaac gaaaacaggc gcggctcgag cgggggcaac gtgatgccgt actcccagct
     3661 cacgaggcgc tcgctcatga cgaccggttc agtgccgtcc gtctgatcga tcagcaccgt
     3721 gccctggccg agcttcaacg cgagctcgac cgaatcggtg atgcgaccct tgatgccttc
     3781 gcgcaccacg atgcggtcga ccaccacgtc gaggtcgtat tcggcttcgg gctgtaccgt
     3841 gaaatcgtcg ccgagatcga cctgctggcc gttcagcctc gcgcgaacga aaccttcgcg
     3901 tcgcagtcgc tccagctctt cgcgaacgtc tccctggaac cggcgcatga ccggcgcgag
     3961 caacacgatg cgcgcgccgt cgccgcgatc ggcaatcgca tcgacgatct cttgcacggt
     4021 gtacgcgcga agcaccttgc ccgacttcgg gcaatgaggg acgccgacgc gggcgtacat
     4081 gagtcgcagg taatcggcga tctcggtgac ggtgccgacg gtcgatcgtg ggctctttcc
     4141 gaggcctttc tgctcgatcg cgatcgcagg gctcaaccct tcgatgctct cgacgcgcgg
     4201 cttcggaagc tgatcgagga actgccgtgc gtacgcggag agcgattcga cgtagcgccg
     4261 ctgcccttcg gcatagatgg tgtcgaatgc gagtgacgac ttgccggagc cacttggccc
     4321 cgtaatcacc acgagccggt tacggggcag ttcacagctg acgcctttca ggttgtgctg
     4381 cgacgcacct cggacgacca ggacctcttg catgacccgc gagccggcac atctatcacg
     4441 ctttaattcg agcgcgtgtc tagtcgtacg attccctacc tggtgggcga gtccttttgg
     4501 gcccgcgcag ctcggcgttc ctgattcgtc gggctccagc ccgctccaat cgagaacgcg
     4561 ccgcgcggcc cgaagccgga gaaacgactg cgtccctctc ggttgattgg tcccgacgcg
     4621 aacggcttcg ccctttcggt agagcagcac ttgcagaatc ccggcctaac ggtcggtgcg
     4681 ggcagcgact gcggtttcgt cgcccgttct ggtctagggc gcagtccgta tggttcgact
     4741 ccgccgtcga gcgcgcgccc ctgttgccgg acgggcggcg ttcgactagg tttgcgcgcc
     4801 gtgccggtcg aaacccacgc ccttgcgtcg gtctctccgc ggatttcaga tccgtcggac
     4861 gggcccggcc agcagggggt gcgcggagct ccgcgggccc atagctcaat cggtcagagc
     4921 ccccggctca taaccgggtt gttcctggtt cgaacccagg tgggcccact ggccgtcgcg
     4981 gcctgcttcg tgtgaagctt gtgtagctga tgctgtcccc tgtcaccgtt accgatttcg
     5041 aaactcgctc gaggcgggca agccttcgag atcggcctcg cacagatcga aggagcccat
     5101 tcgaaacgtg actatccagg cgcaaatcga ggtgctcgaa gccctcgcgg ccctagacgc
     5161 agagcttaaa gaacttaacg acgccctcag tctcgagcgc gaagggctca ggagcaagca
     5221 gagtcagctc tcgagtttgg acgagcgctt tgagcggagc cgcaagagcg tcgaagacat
     5281 ggagcgcttg cggaacgatc tgctcggcga attgcggcag atgggcacgc aagtcgagcg
     5341 ctcgcgagag aagctctctc gctgtcgcac ggagcgcgag gccaatgccg ctcagcgcga
     5401 gctcgaagag ctccgcaagc tctatcgcga tcgcgaggtc gagagcgaaa agctcgagtc
     5461 gctcatctcg caggctcgcg aggaaagcgg gcagctctcc accgaccgcc aaaagctctc
     5521 tgccgacttg ggggcgagcg aaggggacgt cgtgggtcgc ctcgagacct ccgagcgctc
     5581 tctctccgag aagctgtcgg ctcgggccga tctggtgaag cgcgtggatc cggcgctcta
     5641 ccgccggtac gagctcgttc gcaaacgcaa ggggagcgcc atcgcgcaca ccagcaccgg
     5701 tacctgcagt gcctgtcaca tggcggtgca gccgatgctt ttccagaagc tcttgcgtag
     5761 cgacgagttc gggcaatgcc cgcactgcaa tcgcattctc tactaccggc ccgcgaaggc
     5821 tggtgagtcc gaggactctt cggagagtgc gtgacgtggc tcgcgcgttg tcgggggcgg
     5881 cgcgcttgcg ccgtggttga cggatgaaag cttgtgcgca gtgcggaagg ctgtaccccg
     5941 atgatgcggg attctgtcca gtcgacggcg ctgcgctcgg gcgcccgacg agcgtgccgg
     6001 ttccggtcga tgcgacggac aagcgcgtag ggcagctcgt ctgccagcgt taccagatcc
     6061 gccgggtggt ggccgatggc ggcatgggcc gcgtttacga ggcgctcgac ctccgcgtga
     6121 agcgcaacgt agcgctcaag atcctccacg ccgaggtggc cgacgacgag gtcgcggtcg
     6181 agcgcttcaa gcgcgagttc gaggtcagta agcagctgcc ccacgactac atcgtcgagg
     6241 tgttggattt tcagccaacg ggcgacaaca cctacgctct cgcgatggag tacctctacg
     6301 gggaagagct ccgcgcgacg ctgaagcgtg aagtagtgat ttctccggcg cggctcgtgc
     6361 ggatgttgag ccagatcgcg attggcctcg acgaagctca cgctcgccag ttcgtgcacc
     6421 gcgatctcaa gccggacaac ttgttcctcg ggcagaccag cgacggcgac atcgtcaaga
     6481 tcctcgactt cggttcggtc aaggacaagg gcgagagcgc gaagaagctg accgtgctcg
     6541 gcaccaccat cggctcgccc ttctacatgg cgccggagca ggcgcagggc ctcgacaccc
     6601 tcgatcatcg cgccgacgtg tgggcgctcg cggcgataac ctacgagtgc gccaccgggc
     6661 aggtgccgtt ccagggcaac aacggcccga gcatcctcct cgagatcctc accaaag
//
LOCUS       DASTKS010000023         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1118483, whole genome shotgun sequence.
ACCESSION   DASTKS010000023 DASTKS010000000
VERSION     DASTKS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118483"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1142
                     /locus_tag="VFQ35_00460"
     CDS             258..1142
                     /locus_tag="VFQ35_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929118.1"
                     /translation="MTSLDAFAPLGRRLSRRAASALILGIGVAALATGCRRSSSVSAA
                     QCKEHVTMLGAVAKQDLEEIRKGLPLGAKFLEEFAAQGKFDDAGASRDALERARNKVQ
                     DLRVAKASFFALVDSQGNVARSDNSPDLLAGKNLFDAFPELRQALSDKYVETRGSIEA
                     ASKIKGGPDAQWVAATPIHANGAVKAVYAAGWSWSAYAYRLQNHLQGKIRSALPERGK
                     EPLTYVYMVVGNSVFGAPISPAVNARAIADQHFLGAPGTEPVVRELEITEREFGLGFL
                     RMPELGSDVGIAVLRSET"
     gene            complement(1158..1400)
                     /locus_tag="VFQ35_00465"
     CDS             complement(1158..1400)
                     /locus_tag="VFQ35_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929119.1"
                     /translation="MTKASGAEVEVEGKPVNEANTRVLLKVGATLTVCGIALAVADDE
                     GIARWLALAGIALLVFGLHRFGRLGSDARWRRDAES"
     gene            complement(1397..1912)
                     /locus_tag="VFQ35_00470"
     CDS             complement(1397..1912)
                     /locus_tag="VFQ35_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929120.1"
                     /translation="MPLEPLLRLVSVPAYMLATFAMACGGPPPRTANPARPLEERRAL
                     DVIAEVFREENETPVAGGDVSLSVQGRLHVDVQAGGKRFGVAYVTGKERSELGRALPA
                     RDPNMGDALQLVSGLGTDGDARVCVLYDTDYLYDDQVGEAHEKSTTTAELKLRRDVRD
                     FLVIAHREHLR"
     gene            2059..2691
                     /locus_tag="VFQ35_00475"
     CDS             2059..2691
                     /locus_tag="VFQ35_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929121.1"
                     /translation="MALGPRWFVRFAGAAALLAGCQRQRVSDPSATIAAYAAAAKRGD
                     ADAIYALLSRRAQRDLGREGTRQRVLDARHELAAQADFFARPGVEPEVLAVVRYEDGE
                     HAELALEGGLFRVGSASALPAAARTPTEALAGLRQALARRSYVALVRVLSAETRGALE
                     SDVSSIVEGLQDPDTLDVQVSGDDAEVELPSGHSVRLKREAGVWRVEDLK"
     gene            2688..3449
                     /locus_tag="VFQ35_00480"
     CDS             2688..3449
                     /locus_tag="VFQ35_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET9929122.1"
                     /translation="MRFSRRACLTGAAAAVMSAAQRASAFGESSAFNPRMLSAGTRKV
                     LPWAESATGRWAWELLRRTSAPARLVPSRVSADRAELWSEPFAIWSGDADAGTLSNAE
                     LRGLTRFFRLGGVLLVDDAQPASGAFGRSARREIARVLPESPPVRLDPSHVLFKTYYL
                     LDKPVGRVLGAGRLDAIVRGKNAQVLFSDVDLLGALARNGEDWAFEVAPGGVEQRERA
                     IRLCVNIAMYLLCSDYKDDQVHAEWLMRRRASPRR"
     gene            3449..>5496
                     /locus_tag="VFQ35_00485"
     CDS             3449..>5496
                     /locus_tag="VFQ35_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET9929123.1"
                     /translation="MATHFSIAEDLHATALVLAVVVMTSWLALLVWEVLRQRSRRGLV
                     LGTGLIAFALVTLSVLRPVRVKSRASRVGPRVVVLADRSRRLLLPADDANRTREAVLR
                     SSLPSLMRALEGARLSVLGFGGGEPAPFASADQRPPSEKDSDLSQALESLARAPGERP
                     EAVIVLSDGRLLRPGEKASDAELEHLAGRLSVPIHVVRLAEQALPDASLRSVHNAGVA
                     VAHQPLALRLEIACSGGLRCEKIPVRIRELKQGEAPVLLADGVAEFGGKDVATLELGI
                     TLERAGTRIVDVDIQAPDGDRIPDNDRRLLTFNVTRERVRLLHVAGRPSYDVRSLRMW
                     LKGNDSIDLVAFFILRTHQSDPNTTDQSEELSLIPFPVHDLFTEHLPSFDAVVLQDID
                     AVAYDLAQYLPALERYVRSGGGLIMVGGPSSFAGGGYARSALERVLPTELGDPAHPFD
                     LAEFVPAYTDAGRVAPVLGPLRELVGGDLPKMVGANLLGRPKQSAIVLWERAARRAGE
                     APLPVLALNEAGDGRTISLAIDGTHQLAFSEFAERTAGRAYGALWDGLLGWLMRDPRY
                     EAARIELSSDCIAGEMARLRLIRLPGAEGDVDLDVESLSADSNPRLTKRAHVPASGVA
                     EIEVGPLATGGYTVRARVGQAPPARFDFACERGGEAFRDSRPDPELLNRIARASAGRA
                     V"
BASE COUNT          825 a         1837 c         1905 g          929 t
ORIGIN      
        1 aacgagatcc tcaagtcagc ggagggggat cctttagctc aacgcccgcg aatttttgct
       61 cgaaagcgtt cacctcggcg cactcgatcg tacatgccgg ggcgtgtccc actcgcgcga
      121 cggcgctcgc gaaagagtcg cgctcgtttc ggacggcgtc gtggggccgc gccgtcgacg
      181 cgttcgaatg gccaatttcg cggtgaaatg cgcaaaatct gtcgcggggc cgcgccccga
      241 ttatgctcgg cgcgaccatg acgagtctcg acgcattcgc cccactcggc cgccggctgt
      301 cacgccgcgc tgcctcggcg ctcatcttgg gcatcggtgt cgcagctttg gctacgggct
      361 gccgtcgctc gagcagcgtc agcgccgcgc aatgcaaaga gcacgtcacg atgctcggcg
      421 ctgtcgcgaa acaagacctc gaagagattc gcaaaggctt gcccctcggc gcgaagttct
      481 tggaagagtt cgcggcgcaa ggaaagttcg acgacgcggg cgcatcgcgc gacgcgctcg
      541 agcgggcgcg caacaaagtg caagaccttc gcgtcgcgaa ggcgagtttc ttcgcgctcg
      601 tcgattcgca agggaacgtc gcgcgcagcg acaacagccc cgacctcctc gcaggtaaga
      661 atctgttcga tgcgttcccc gagcttcggc aggccctgag cgacaagtac gtcgagacgc
      721 gcgggagcat cgaagccgct tcgaagatca aaggcggccc agacgctcaa tgggtcgcag
      781 cgacgccgat acacgcgaac ggcgcggtca aggccgtgta cgccgcgggc tggtcatggt
      841 ccgcgtacgc gtaccgcttg cagaatcatc tacagggcaa gatccgaagc gcgctgccag
      901 agcgaggcaa agagccgctc acctacgttt acatggtcgt tggcaacagc gtcttcggcg
      961 cgcccatttc tcccgcggtg aacgcccgcg cgatcgccga ccagcacttt ctcggcgcgc
     1021 ccggcaccga gccggtcgtg cgcgagctcg agatcaccga gcgcgaattc gggctcggct
     1081 tcttgcggat gcccgaactc gggagcgacg tcggcatcgc agtgctgcgg agcgagacgt
     1141 agcgagtgaa gcgcgactca gctctcggcg tcgcgacgcc agcgcgcgtc gctgccgagg
     1201 cggccgaacc gatgcaggcc aaacacgagc agcgcgattc cggccagcgc tagccaccgc
     1261 gcgatcccct cgtcgtcggc gaccgccagc gcgatgccgc aaaccgtcag cgtcgcgccc
     1321 accttgagca gcacgcgcgt gttggcctcg ttgacgggtt tgccctcgac ctcgacttcc
     1381 gcgcccgagg ctttggtcat cggaggtgct cgcggtgggc gatgaccaag aagtcgcgca
     1441 cgtcccgccg cagcttgagc tcggcggtgg tggtgctctt ctcgtgcgct tcgccgacct
     1501 gatcgtcgta gagatagtcc gtgtcataga ggacacagac gcgcgcgtcg ccgtccgtac
     1561 cgaggccaga gacgagctgc agcgcgtcgc ccatgttcgg gtcccgagca ggcaacgccc
     1621 ggccgagctc gctccgctcc ttgcccgtga cgtacgcgac gccgaagcgc ttgccccccg
     1681 cctgcacgtc gacgtgcagc cgcccttgga ccgagagcga aacatctccg cccgcgaccg
     1741 gcgtctcgtt ctcctcgcga aacacctcgg cgatcacgtc gagcgcccgg cgctcctcga
     1801 gagggcgagc tggattcgcg gtgcgcggag gcggcccgcc acacgccatt gcgaacgtgg
     1861 ccagcatgta agccgggacg gagaccagcc ggagcagggg ctcaagcggc attcgttggc
     1921 agaccttatc taagagctca tggcttcgcc agcttcgggt tgcgatcgtt gagctgcacg
     1981 ggcggccgcc gccggccctc cggacgggtc gctgcagcga gttcgccgtg ggcgcgcggc
     2041 tgctatagct tttgcgccat ggccctcggt cctcgttggt tcgttcgctt cgcgggcgcc
     2101 gccgctctgc tcgcggggtg ccagcgtcaa cgcgtttcgg acccgagcgc tacgatcgcg
     2161 gcgtacgcgg ccgcagccaa gcgcggcgac gccgacgcga tctacgcgct tttgtcgcgt
     2221 cgcgcgcagc gggatttggg gcgcgaaggg actcggcagc gagtactcga tgcgcgtcac
     2281 gagctcgcgg cgcaggcgga tttcttcgcc cgccccggag tcgagccgga ggtcttggcc
     2341 gtcgttcgct acgaggacgg cgagcacgca gagctcgccc tcgagggagg gctcttccgc
     2401 gtcggctccg cctcggctct gcccgctgcc gcacgaaccc cgaccgaggc cttggccggg
     2461 ctgcggcagg cgctggcgcg gcgcagctac gtcgcgctcg ttcgagtgct ctcggcggag
     2521 acgcgtggcg cgctcgagag cgacgtcagc tcgatcgtcg agggcttgca ggatccggac
     2581 acgctcgacg tgcaggtgag tggcgatgac gcggaggtgg agcttccttc tgggcacagc
     2641 gtgcgcctca agagggaagc gggcgtttgg cgcgtcgagg atttgaaatg aggttttcgc
     2701 gccgagcttg tctgactggc gcggcggcgg cggtgatgtc ggccgcgcag cgcgcgagcg
     2761 ccttcggcga gtcgtccgct ttcaatccgc gcatgttgtc ggccggtacc cgcaaggtgc
     2821 tgccttgggc cgagagcgct actggacgtt gggcgtggga gctcttgcgc cggacgagtg
     2881 cgcccgcgcg cttggtcccc tcgcgcgtct ccgccgatcg cgccgagctc tggagcgagc
     2941 cgttcgcgat ttggtccggc gacgccgacg ccgggacgtt gtcgaatgcc gagctgcgtg
     3001 ggctcacgcg attcttccgt ctgggtgggg ttctcctcgt cgacgacgct cagcccgcga
     3061 gcggcgcgtt cgggcgctcg gcacggcgtg aaatcgcgag ggtgttgccc gagtcaccgc
     3121 cggtacgact ggatccgagc cacgtcttgt tcaagaccta ctacctgctc gacaaaccgg
     3181 ttgggcgcgt cctcggggcg gggcgtctgg acgcgatcgt ccgcgggaag aacgcgcagg
     3241 tgctgttctc cgatgtcgac ctactcggtg cgctggcgcg aaacggtgaa gactgggcgt
     3301 tcgaagtcgc gccgggcgga gtcgagcagc gcgaacgcgc catccgcctg tgcgtgaaca
     3361 tcgcgatgta cctgctctgc tccgactaca aggacgacca ggtccacgca gagtggttga
     3421 tgcgccgtcg agcgagtccg cgtcgctgat ggccactcac ttttcgatcg cagaagatct
     3481 gcacgcgacc gcgctcgtgc tcgccgtcgt cgtgatgacg tcgtggctcg cgctgctcgt
     3541 ctgggaggtg ctacggcagc gctctcggcg cggtctggtg ctcggcacgg ggctcatcgc
     3601 gttcgcgctg gtgacgctca gcgtgctgcg cccggtgcgg gtgaagtctc gagcgagtcg
     3661 cgtcggccca cgcgtcgtcg tcctcgcgga tcgctcgcgg cgcttgttgc tgccggccga
     3721 cgacgcaaac cgaacgcgtg aggcggtttt gaggagctca ctgccgtcgc tcatgcgcgc
     3781 gctcgaaggg gcgcggctca gcgtgctggg ctttggggga ggggagcctg cgccgtttgc
     3841 cagcgccgat cagcgcccgc cgagcgagaa ggacagcgac ctctcgcaag cgctggagtc
     3901 gctcgcccgc gcgcccggcg agcgcccgga agccgtcatc gtcttgagcg acggacggct
     3961 acttcgcccg ggagagaaag cctccgatgc agagctggaa cacctcgcgg ggaggctcag
     4021 cgtgccgatt cacgtcgtgc ggctcgcgga gcaagcgctg ccggatgcgt cgctgcgcag
     4081 cgttcacaac gccggcgtcg ccgtcgcgca ccagccgctc gctttgcgcc tcgaaatcgc
     4141 ctgcagcggc ggtctacgtt gtgaaaagat cccggtgcgc attcgcgagc tcaagcaggg
     4201 cgaagcgccg gtgttgctcg cggacggcgt ggccgagttc gggggcaagg acgtcgcgac
     4261 gctcgagctc ggaatcactc tcgagcgcgc gggaacgcgc atcgtggacg tcgacattca
     4321 agctccggac ggagatcgca tccccgacaa cgaccggcgg ttgctcacgt tcaacgtcac
     4381 gcgcgaacgc gtgcgtctcc tgcacgtggc gggccgaccg agctacgacg ttcgctcgtt
     4441 gcgtatgtgg ctgaagggca acgactcgat cgacttggtc gcgtttttca ttctgcgcac
     4501 gcatcagagc gatccgaaca ccaccgatca aagcgaggag ctctcgctga tcccatttcc
     4561 tgtccacgac ctcttcacgg agcacctgcc gagcttcgac gcggtcgtgc tgcaggacat
     4621 cgacgcggtc gcgtacgacc tcgctcagta ccttccggcg ctcgagcgct acgtgcgctc
     4681 tggcggtggt ctcatcatgg tgggcggccc ttcttcgttc gccggtggtg gatacgctcg
     4741 ctcggcgctc gaacgcgtgc tgccgacgga gctcggagat cccgcgcacc cattcgacct
     4801 cgccgagttc gtccccgctt acaccgacgc agggcgcgtc gcgccggtgc tcgggccctt
     4861 gcgcgagctc gtgggcggcg acttgccgaa gatggtcgga gcgaacctac tcggaaggcc
     4921 gaagcaatcc gcgatcgtgc tgtgggagcg agccgcaagg cgggcgggcg aggcgccgct
     4981 ccctgttctc gctttgaacg aagccggtga cgggcgaacc atttcgctcg ctatcgacgg
     5041 aacgcaccag ctcgcgttca gcgaattcgc agagcgcacc gccgggcgcg cctacggcgc
     5101 cttgtgggac ggcttactcg gctggctcat gcgcgacccg cgctacgaag cggcgcgcat
     5161 cgagctttcg agcgactgca tcgcggggga aatggcgcgg cttcggttga ttcggctgcc
     5221 cggcgcggag ggcgacgtcg acttggacgt ggagtctctc tcggccgact cgaatccgcg
     5281 cctcacgaag cgtgcgcacg tgccggcctc gggcgtggcc gaaatcgaag tcggccccct
     5341 cgcgacgggt ggctacacag tccgcgcgcg tgtcggacag gcgccccccg cgcgcttcga
     5401 tttcgcctgc gagcgcgggg gcgaggcgtt ccgcgattca cgccctgatc cggagctcct
     5461 caaccggatc gcgcgggcga gcgccggtcg ggccgt
//
LOCUS       DASTKS010000024         7314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1564493, whole genome shotgun sequence.
ACCESSION   DASTKS010000024 DASTKS010000000
VERSION     DASTKS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564493"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFQ35_00490"
     CDS             complement(<1..796)
                     /locus_tag="VFQ35_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9929124.1"
                     /translation="MSDPELAESSAKKFTHPRSARDVYGAAVGVVALLGAFSYFAFAR
                     APQKPNGASGGAVRVERVHSFGNAARAEACAPSGTPCEKLVNGSSIPAGSHLRTDFQT
                     ELALSLGDKGHLILARGSELELLGASKGTARLRRGSLFGELEDTRGTFELELPHGTLS
                     ATRAKFAAHAESDASRVEVSRGNVRVENTDGHASPVHAGEVARFSKTRFDVEPGQVLG
                     SALAFDDDADSPRAERQTRGLGELSARKPGSREELGGAVHLVSHAVK"
     gene            928..1893
                     /locus_tag="VFQ35_00495"
     CDS             928..1893
                     /locus_tag="VFQ35_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9929125.1"
                     /translation="MSFRHLSTIAAAPRFSPTLSAVPELPDVTVYVERILALFGGRRL
                     DAVRIKSPFVVRTYEPPIQSLVGRTLTNTFSVGKRIVLEFEPELYVVVHLMIAGRFHL
                     KERSVALGKVTLLAFDFENQTMHLTEASSKKRASVHVFATREEVQSLARGGVEPLTAT
                     FAEFKEALDFENHTLKRTLTDPRIVSGVGNAYSDEILHRAQLSPMKRTKDLNEDDAQR
                     LYEAMQSTLTEWVDRLRADSQAGFPEKVTAFRPEMAVHGKYNHPCPVCGTKVQRIVYA
                     ENECNYCPTCQTGGKLLADRALSRLLKEDWPKTLEELEERKTPRV"
     gene            complement(1919..2368)
                     /locus_tag="VFQ35_00500"
     CDS             complement(1919..2368)
                     /locus_tag="VFQ35_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9929126.1"
                     /translation="MAFDRILVPVDYSAHSLKAVGYAAELAQRLGASLDIVHVWDRPT
                     YISDAILVGHGADQRSLAEMIRENAQNDMKDFLSKVKLATGVKTTDRLLSGDPATVIL
                     QELERDQYDLVVMGTHGRGGLAHLLLGSVAEKLVRHSKVPVLTVPRA"
     gene            2521..2901
                     /locus_tag="VFQ35_00505"
     CDS             2521..2901
                     /locus_tag="VFQ35_00505"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9929127.1"
                     /translation="MSQRKPDFELEATLVKDHSVIGGVTRESVPPRLELVRGPLAPRT
                     LPLGPDETVVGRSHQANICIESSLLSRRHVAIKKMGPEFRLSDLDSANGVFLNGVRVH
                     SASLHEGDTIQIGDVVFVFREGVA"
     gene            2906..3793
                     /locus_tag="VFQ35_00510"
     CDS             2906..3793
                     /locus_tag="VFQ35_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9929128.1"
                     /translation="MPAFVRFWGTRGSIPTPGHGTRRYGGNTSCVEIRFGETLFICDG
                     GTGLRELGLALQQRDTAGVEAHMFFSHMHWDHIQGFPFFVPAYAPKNHVHVYEVQESD
                     TRVQRLLHGQMRSEYFPVTFADLGATISAGFFERGELRIDGVTIRCLEQNHPGKSYAY
                     SFEKDGTKVVYATDSELDLVLQNRDESLRDLGRARELPKEILEFVDGADLLIADAQYT
                     DAEYPSRVGWGHARANTVVDLALQAGVKQCALFHHDPMQNDEAVEQKVAAARERASRV
                     ASSPVLFGAREGLELKLGG"
     gene            3978..4460
                     /locus_tag="VFQ35_00515"
     CDS             3978..4460
                     /locus_tag="VFQ35_00515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9929129.1"
                     /translation="MKVLLPAHCVSRHPIRVRFGETDLMGIVHHGTYISYFEVGRVEY
                     MRRRGLNYNSWTEMGFHLPVVEAYVRYRKSVRFDELVVLETRLTELMRVKVRFDYRLW
                     RPLNDGGDEELLAEGHTSLACVGRDHVLRRLPPEAEAVLTGPEVEETTPEVPRTAAVR
                     "
     gene            4514..6151
                     /locus_tag="VFQ35_00520"
     CDS             4514..6151
                     /locus_tag="VFQ35_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929130.1"
                     /translation="MAERRDPKQPRASLVDSGWASEPPPPVDVAPPAVSSPRAASRRP
                     PPPARPSSLPPPPPSLPPPPVPSVPPATPASLPPLPPAGRRLSQPPPKPSRPPAPGSV
                     PPPPPSPVASVPPPIAPAPIVTPVATSPVSASVPAGAPLTPAVQAPSPIPPPPPAPVT
                     LPSNTPPALEQPRAERRVATEPPVVLAGPSAASVHRPRELAAYVAESKPHIESTHVDR
                     TVEARARAMLATESGTAAYMAPDVRDMHDDEHEYGDRPVLPGFGAAMAVRVRFMGGEV
                     PLWSLFAPVVIVVALAAALAASAASHAPAQQSGDDAASRPLPSASSEPTTASAAQAER
                     AAPARSAENTTPSPEGFDLPAGSYTAKDVLALAAKRSARDIAAAQALAASLDRDPAAI
                     QEPRVLNDLRRYAENPDTAKLVLESMAKLPAPLAPDLLYEVWTGTPERNDVTELARAL
                     LFSKDVRPKASNSLAVALDLRTAETCEQNAQILPRAIKDADRRSLHLLIKLQRKYGCG
                     PNKRLDCYPCLRSGDQLESALKTSREHPEPRPFGGKR"
     gene            complement(6223..6669)
                     /locus_tag="VFQ35_00525"
     CDS             complement(6223..6669)
                     /locus_tag="VFQ35_00525"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9929131.1"
                     /translation="MEVRTEIEISAPPAQIWKVLLDFRRYPEWNPFIVHLAGEPNVGS
                     KLAMTLSLPDSNREREVTSRLLRCDTEQELRWLGHLWMKGLFDGEHFLRLEPRDDGTT
                     RIVHGEDFHGIFLRFMMKQVTETTRGFVYMNQALKKRVEQGARRAR"
     gene            6757..>7314
                     /locus_tag="VFQ35_00530"
     CDS             6757..>7314
                     /locus_tag="VFQ35_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929132.1"
                     /translation="MRLIRALIRGSGPFERVELPFADESGSPRLLTVIHGGGGVGKST
                     VIQAIASTRPGHAMAQLSRSESAGTPHVACDWLLGADDPTRPHPLTVVSPNARFYNEE
                     DREALRRREQVHYDRIAQDGGFVFLGIPSTRWFSRQPITISAPARGVARYDVRTPLAL
                     DDAARADLARETKQSLAYAAIAVALA"
BASE COUNT         1227 a         2461 c         2392 g         1234 t
ORIGIN      
        1 ccttgaccgc gtgactgacg aggtgcacgg cgccgccgag ttcttcacgg ctaccaggct
       61 tgcgagcgct gagctcaccg aggccgcgcg tttgccgctc ggcgcgcggc gagtccgcgt
      121 cgtcgtcgaa agcgagtgct gagcccaaaa cctgccctgg ctcgacgtcg aaacgcgtct
      181 tcgaaaagcg cgcgacctcg cccgcatgca cggggctcgc gtggccgtcg gtgttttcga
      241 cgcgcacgtt gccgcgcgaa acttcgaccc gcgaagcgtc gctctcggca tgggcggcga
      301 acttcgcgcg cgttgcgctg agggtgccgt gcggtagctc gagttcgaag gtcccccggg
      361 tgtcctcgag ctcgccgaac agcgagcctc gcctcagccg tgcggtcccc ttcgaggcgc
      421 cgagcagctc gagttcgcta ccgcgcgcga ggatcaggtg acccttgtcg ccgagagaaa
      481 gggcgagctc ggtttggaag tccgtgcgga ggtgcgagcc ggcggggatg ctcgagccgt
      541 tcaccagttt ctcgcatggc gtcccgctcg gcgcgcacgc ctccgctctg gcggcattgc
      601 cgaagctgtg cacgcgctcc acccgaaccg cgcccccgga ggcgccgttg ggcttttgcg
      661 gcgcgcgagc gaaagcgaag taactgaacg cgccgaggag cgccaccact ccgaccgcgg
      721 ctccatagac gtcgcgcgcg ctccgcggat gagtgaactt cttggcagac gattcggcga
      781 gctcagggtc cgacacgggg gtcctcctca acgggaacga tgagaatcgg cacgcacagg
      841 aacgctcgtc cgcgacggaa ctttcagagc cgcgtgaccg ttggcactcc acagcgctcg
      901 gatgagcgtc gtcttggtag aaatcccatg agtttccggc acttgtcgac gatcgcggca
      961 gccccgcgat tttcgcctac gctgagcgcc gtgcccgagc tccccgacgt tacggtctat
     1021 gtagagcgca ttctggcctt gtttggcggt aggcggctcg acgcggtgcg catcaagagt
     1081 cccttcgtcg tgcgaaccta cgagccaccg atccagtcgc tggtaggtag gacgctgacc
     1141 aacactttca gtgtaggtaa gcgcatcgtg ctcgagttcg agcctgagct ctacgtggtc
     1201 gtacacctca tgatcgcggg gcggtttcac ctgaaggagc gcagcgtcgc cctcgggaaa
     1261 gtgacgctgc tcgcgttcga cttcgaaaat cagacgatgc acctgacgga agcgagcagt
     1321 aagaagcgcg cctctgtgca cgtgttcgcg acgcgcgaag aggtgcagtc gctcgcgcgg
     1381 ggaggggtcg agccgctcac ggcgacgttc gccgagttca aggaggcgct cgacttcgaa
     1441 aaccatacgc tcaagcgcac gctcaccgat ccacgcatcg tgagcggcgt gggcaacgcc
     1501 tactcggacg agatcctcca tcgcgcgcag ctctcgccga tgaagcgcac caaagatctg
     1561 aacgaagacg acgcgcagcg gctctacgag gccatgcaga gcacgctgac ggaatgggtc
     1621 gaccgcctgc gcgccgacag tcaggctggc tttcccgaaa aagtcaccgc ttttcggccc
     1681 gagatggcgg tacacggaaa gtacaaccac ccttgcccag tctgcggcac caaagttcag
     1741 cgcatcgttt acgccgagaa cgagtgcaac tactgcccaa cgtgccagac gggagggaag
     1801 ctcctcgccg atcgcgcgct ttctcgtcta ctgaaagaag actggccgaa gacgctagaa
     1861 gaactcgagg aacggaagac gccgcgcgtg tgagcctaga cgtcgccccg cgatggcgtc
     1921 aggcgcgcgg caccgtgagc acgggcacct tcgagtgccg gacgagcttc tccgcgacgc
     1981 tgccgagcaa aaggtgggcg agcccaccgc gcccatgcgt acccatgacc actaggtcgt
     2041 actggtcgcg ctcgagctcc tgcaagatca cggtcgctgg atcaccggag agcaggcgat
     2101 cggtcgtctt cacgccggtc gcgagcttca ccttcgagag gaagtccttc atgtcgttct
     2161 gcgcgttctc gcggatcatt tcggcgagag agcgttggtc ggcgccgtgc ccgacgagga
     2221 tcgcatcgga gatgtaggta ggccggtccc agacgtgaac gatgtcgagg ctcgcgccga
     2281 gacgttgcgc gagttcggcc gcataaccga cggccttcag cgaatgagcc gagtagtcca
     2341 cgggcacgag gattcgatcg aaggccatga cttgctgaac gtagtgctca aagtggctgc
     2401 tttcaattga tgtaagtcac cgagcgcggc ttttccgggc ggccgcttcg tgcgcggacg
     2461 gaaaatcgcg ctcgtcgttt cagttcacca tggggaacga aggtgtaaac tagagggccg
     2521 gtgtcacagc gcaaaccaga cttcgaactc gaggcgactc tggtgaagga ccacagcgtc
     2581 attgggggag tgacgcgcga atcggtgccg ccgcgcctcg agctcgtccg gggtccgcta
     2641 gcgccgagaa cgctgccgct cggtcccgac gaaaccgtgg tcgggcgctc gcaccaggca
     2701 aacatttgca tcgagagcag ccttctctcg cgccgccacg tcgcgatcaa gaagatgggt
     2761 ccggaatttc gcttgagcga tctcgacagc gcgaacggcg tcttcctgaa cggcgtgcgc
     2821 gtgcactcgg cgagcctgca cgagggcgac acgattcaaa tcggcgacgt ggtgttcgtc
     2881 ttccgagaag gggtggcctg agcgcatgcc ggccttcgtg cgcttctggg gaacgcgcgg
     2941 ctctatcccg actccgggtc acggtacgcg acgttacggc ggcaacacct cgtgcgtcga
     3001 gattcgcttc ggtgaaacgc tgttcatctg cgacggcgga acgggtttgc gcgagctcgg
     3061 cctcgcactc cagcagcgcg acaccgcggg ggtcgaagcg cacatgttct tcagtcacat
     3121 gcactgggac cacattcaag ggttcccgtt cttcgtcccc gcgtacgctc cgaagaatca
     3181 cgtgcacgtt tacgaagttc aggagagcga cacgcgtgtt cagcggctgc tccacggcca
     3241 aatgcgctcc gaatacttcc ccgtgacctt cgcggatctg ggcgcgacga tcagcgcggg
     3301 cttcttcgag cgaggcgagc tccgcatcga tggcgtgacg attcggtgtc tcgaacagaa
     3361 ccaccctgga aagagctatg cgtactcgtt cgagaaggac ggcacgaagg tggtctacgc
     3421 gaccgacagc gagctcgacc tcgtgcttca gaaccgcgac gagtcgctgc gggacctcgg
     3481 gcgcgcgcgg gagttgccga aagagattct cgagttcgtc gacggagcgg acctgctgat
     3541 cgccgatgcg cagtacaccg acgccgaata tccttcgagg gttggttggg ggcacgctcg
     3601 cgcgaacacc gtcgtcgatc tcgcgttgca ggccggcgtg aagcagtgcg cgctgttcca
     3661 tcacgacccg atgcagaacg acgaggcggt cgaacagaaa gtggcggcgg cccgagagcg
     3721 ggcgagccgc gtggcgtcga gcccggtgct cttcggcgcc cgcgaggggc tcgagctcaa
     3781 gttgggtggg taggtcgccc cttcccagag gctgacttgg ggagctggcg ccctgacccc
     3841 actccgctcc tatagcgaca gggtggccgg ccgactttgc gcgaccgccc agtttggccg
     3901 aacccgagcg atcttcgcgt cacgttgaaa ctcggcttgc cggagtgcgt ggcacgcggc
     3961 tattttcgcg cgcctccgtg aaagtcctgc tacccgcgca ttgcgtttct cggcatccga
     4021 ttcgcgttcg ctttggcgag accgacctga tggggatcgt ccaccacggt acctacatca
     4081 gttacttcga ggtgggccgg gtcgagtaca tgcgacggcg cgggctcaac tacaattcgt
     4141 ggaccgagat gggcttccac ctgcccgtcg tcgaggcgta cgtgcgttac cggaagagcg
     4201 tgcggttcga cgagctcgtc gtgctcgaga cgcgtctcac cgagctgatg cgcgtgaagg
     4261 tgcgcttcga ttaccggctg tggcggccgt tgaacgacgg aggcgacgag gagctgctgg
     4321 ccgaggggca cacctcgctc gcgtgtgtcg gtcgggacca tgtcttgagg cgcctgcctc
     4381 ccgaggcgga agccgtgctc accggacccg aggttgagga aacgacccct gaggtcccac
     4441 gtacggcagc cgtacgttga aagccgtggg cgtccttccc aagccggcgc tgctcgcgcg
     4501 ataatcaaag cctatggccg aacgccgcga tcccaagcag ccccgggcgt cgctcgtgga
     4561 tagcggctgg gcgagtgagc cgccgccgcc cgtggatgtt gctccgcccg cggtgtcatc
     4621 gccgcgcgcg gcttcgcgtc gaccgccgcc gcccgcgcgc ccttcatcgt tgcctcctcc
     4681 gccgccttcg cttccgccac caccggtgcc ttcagtgcct cccgctacgc cggcgtcgtt
     4741 gcctccgttg ccgcccgcag gtcggcgctt gtcgcagcct ccgccgaaac cttcgcgacc
     4801 gccggcaccg gggtctgttc caccgccacc gccgtcgccg gttgcatcgg tgcctccgcc
     4861 gattgcgccg gcgcccatcg tcactccggt cgcgacctct cccgtgtcgg cgagtgtccc
     4921 cgccggcgct ccgctgacgc ccgcggttca agcgccatcg ccgattcctc ctccgccccc
     4981 cgcgccggtc acccttcctt cgaatacgcc accagcgctc gagcagccgc gtgcggagcg
     5041 gcgtgtcgcg acggagccgc cggtcgtgct ggcagggccg agcgcggctt cggtccatcg
     5101 tccgcgcgag ctcgcggcat acgtcgcgga atccaagccg cacatcgagt cgacgcacgt
     5161 cgatcggacc gtcgaagcgc gcgcgcgcgc gatgctcgcg acggagtcgg gcacggccgc
     5221 gtacatggcg ccggacgtgc gcgacatgca cgacgacgag cacgagtacg gcgatcgccc
     5281 cgtgttgccc gggttcggcg ccgccatggc cgttcgcgtt cgcttcatgg gaggagaggt
     5341 gccgctctgg agcctcttcg cgccggtcgt catcgtcgta gcgctcgcag ccgcgctcgc
     5401 cgcttcggct gcgagtcatg ccccggcgca acagagcggt gacgacgcag cgtcccgccc
     5461 gctgccgtcg gcgagtagcg agcccacgac cgcgtcggcg gcgcaagccg agcgcgccgc
     5521 gccagcacgc agcgccgaaa acacgacccc ttcacccgaa ggcttcgatt tgcctgccgg
     5581 gagctacacg gccaaagacg tgctcgcgct cgcggcaaag cgtagcgcgc gtgacatcgc
     5641 cgccgcacaa gcgctcgccg cgtcgctcga tcgcgacccg gccgcgattc aagagccgcg
     5701 cgtgttgaac gacctgcgcc gctacgcgga gaacccggac accgcgaagc tcgtgctcga
     5761 atcgatggcg aagctccccg cgccgctcgc tcccgatctg ctctacgagg tgtggaccgg
     5821 cactccggaa cgaaacgacg tgaccgagct cgcgcgcgcc ctgttgttca gcaaggacgt
     5881 tcgcccgaag gcatcgaact cactcgcggt ggcgctcgac ttgcgcaccg ccgagacctg
     5941 cgagcagaac gcgcagatcc tcccgcgcgc gatcaaggac gccgatcggc ggtcgttgca
     6001 tctgctcatc aagctgcagc gtaagtacgg ctgcggtccg aacaagcgcc tcgactgcta
     6061 cccgtgcctt cgcagcggcg atcaactcga gagcgctctc aagacctcgc gcgagcaccc
     6121 cgagccacga ccgttcggcg gcaagcgctg agcgccgacc gggccggggg cgccgagcgc
     6181 ccagaaggcg cacgggctcg gatgccgcag gtcgaccgtc ggtcagcgag cgcggcgcgc
     6241 gccctgctcg acgcgcttct tgagcgcctg attcatgtag acgaagccgc gcgtcgtctc
     6301 cgtcacctgc ttcatcatga agcgcaggaa gataccgtga aagtcctccc catgcactat
     6361 gcgggtcgtg ccgtcgtcgc gcggctcgag ccgaaggaag tgctcgccgt cgaaaagccc
     6421 cttcatccaa aggtgcccca gccagcgtag ctcttgctcc gtatcgcagc gaagcaagcg
     6481 cgacgtgacc tcccgctctc gattgctatc cggcaagctc aaggtcatcg cgagcttcga
     6541 gccgacgttc ggctcgcccg cgaggtgcac gatgaacggg ttccactccg ggtaccggcg
     6601 gaagtccaac agcaccttcc agatctgggc ggggggcgcg gaaatctcga tctctgtacg
     6661 cacttccatc gggccgctcc ggattctgtc gaattcccct ggcggccgct accggctttt
     6721 tcggctcgaa agctgtgata gcctttcggc gaaagcatgc ggctgattcg tgcgctcatc
     6781 cgtggctcgg gccccttcga gcgagtcgag ctgcctttcg ccgacgagag tggcagcccg
     6841 cggctcctca ccgtgattca cggcggtggt ggggtgggga agagcaccgt gatccaagcc
     6901 atcgcgagca cgcggcccgg ccacgcgatg gctcagctca gccgttcgga gagcgcgggc
     6961 acgcctcacg ttgcctgcga ttggcttctc ggagcggacg acccgacgcg cccgcatccg
     7021 ctcacggtcg tgagccccaa cgcgcgcttt tacaacgagg aggatcgtga agccctgcgg
     7081 cgccgcgagc aggtccacta cgaccgcatc gcgcaagatg gcggcttcgt tttcctgggg
     7141 atcccgagca cgcgctggtt ctcgcggcaa cccatcacga tcagcgctcc ggcgcgaggc
     7201 gttgcgcgtt acgacgtgcg aacgccgctc gcgctcgacg acgccgctcg cgccgatttg
     7261 gcccgcgaga ccaagcagtc gttggcctac gctgcgatcg cggtcgcgtt ggcc
//
LOCUS       DASTKS010000025         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1564702, whole genome shotgun sequence.
ACCESSION   DASTKS010000025 DASTKS010000000
VERSION     DASTKS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564702"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFQ35_00535"
     CDS             complement(<1..276)
                     /locus_tag="VFQ35_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929133.1"
                     /translation="MRKPLSAVGLCLFVLVGCAPQEPAVAPLAAPPPAQPAQPVATTP
                     AQEAPKEAPKPVELTAEQNAKAYADCWAAFNAKDWAKFSACFNEGATT"
     gene            458..934
                     /locus_tag="VFQ35_00540"
     CDS             458..934
                     /locus_tag="VFQ35_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9929134.1"
                     /translation="MQISRMKLSNKGRYAVRALFDIAFFNEGRPTQVKDIAERQNIPP
                     RFLEQIFQDLKRAGIVGSKRGPQGGYSLGRRAGEIRLGDVVRALEGPIVLGDRESAQR
                     KASPASEARRVTEAVFRDLSARVESCFDAVTIADICGRAEELGLKRPGTHRYVYVI"
     gene            918..1892
                     /gene="cysK"
                     /locus_tag="VFQ35_00545"
     CDS             918..1892
                     /gene="cysK"
                     /locus_tag="VFQ35_00545"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET9929135.1"
                     /translation="MSTSSESKLPPRSPHPLVFSNLLELVADTPLVEVRRLGRDAPRA
                     NLYGKLESANPGGSVKDRICLAMIERAEQEGKLGPGGTVVEPTSGNTGIGLALVCAAR
                     GYRCVLTMPESMSLERRQLLESFGAKLVLTPAQEQMEGAIRRAREIVAETPGAFMPQQ
                     FENSANPEVHARLTAREIIHAMGGERVDAFVAAVGTGGTVSGVGRVLKETFPGCRVIA
                     VEPESCATISRGERGPTKIQGIAAGFVPKNYDPSVPDEVRTVSDERAYQTKRELAQRE
                     GLLVGISAGANVAVALDVARELGEGKTVITVFCDTGERYFSLDEYFKP"
     gene            1889..2647
                     /locus_tag="VFQ35_00550"
     CDS             1889..2647
                     /locus_tag="VFQ35_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HET9929136.1"
                     /translation="MSRARPLRSSAGVLQGRRALVVGAGGLGTPALLALADSGATLVI
                     ADDDVVELSNLHRQILFSESDVGRHKLDAVRDALEKEGVSPTRIELVRSRFLPENAVE
                     LARGVDIVLEGSDNFATKFLAADAAHLAGRPVVHGAAVRLEGTVWCVPARGAPCYRCL
                     FEDVPGGAQQGCSEAGVLAPVVGICGALMAELALRALCGLDAYEGSLIAVNGRRNRLR
                     RVPVAARQSCPLCGEAPSIFEIVETRYTAPSCAA"
     gene            2666..2938
                     /locus_tag="VFQ35_00555"
     CDS             2666..2938
                     /locus_tag="VFQ35_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9929137.1"
                     /translation="MATVRIPTPLRTLTKGEEQVQASGDTVRAVIETLEKDHPGIRER
                     LLDEKGVRRFVNIYVGDEDIRFLDGLDTQLKSSDEISIVPAIAGGV"
     gene            2943..3221
                     /locus_tag="VFQ35_00560"
     CDS             2943..3221
                     /locus_tag="VFQ35_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929138.1"
                     /translation="MNERLDRQVRLREVGERGQANIERSYARVERGPAARIELTYLVR
                     AGVERASIAEFDEGPFAHESWFEFSGPRNVARGASDALDHLLSCLELP"
BASE COUNT          571 a         1122 c         1118 g          563 t
ORIGIN      
        1 ggtggtcgcg ccctcattga agcaagcgga gaacttcgcc cagtccttgg cgttgaacgc
       61 cgcccagcag tcggcgtacg ccttggcgtt ctgctctgcg gtgagctcga ccggcttggg
      121 cgcctccttc ggcgcctctt gcgcgggcgt agtagcgacg ggctgtgcgg gttgagcggg
      181 aggcggagcc gcgaggggcg ctaccgccgg ttcttggggc gcacacccca cgagcacgaa
      241 aagacacaat ccaacagcgc tgagaggttt tctcatcttc atttcccctt agttcggtga
      301 ccttggcccc ccccttcgac cccgaggcgg ggccgcccat ctagagccag tttgggtggg
      361 accagcaccg cgcatcagcg ccgcggctcc ccgcagcatc gcttctttgc tggttttttc
      421 gcgcctagcc ccggttcttg acaaatccga tgtataaatg cagatcagcc gtatgaagct
      481 gtcgaacaag ggccgctacg ccgtgcgagc gctcttcgac attgcttttt tcaacgaggg
      541 tcggccgacc caagtgaaag acatcgccga gcggcaaaat atcccacccc gcttcctcga
      601 gcagattttc caagacttga agcgcgctgg catcgtcggc tcgaagcgcg gcccgcaagg
      661 cggatacagc ttgggccgcc gcgccggcga gattcgcctc ggggacgtgg tgcgtgctct
      721 ggaagggccc atcgtgctcg gggatcgcga gtcggcccag cgcaaagctt cgcccgcatc
      781 cgaagcgcgc cgggtcaccg aggcggtgtt tcgcgatctg tcggcgcgcg tcgagtcctg
      841 tttcgacgcg gtcaccattg cagacatttg tgggcgcgcc gaggagctcg ggttgaagcg
      901 tccgggaacg catcgttatg tctacgtcat ctgagagcaa gcttccgccg cggtcgccgc
      961 atccgctcgt cttctcgaat ctactcgagc tcgtcgccga cacgccgctc gtcgaagtga
     1021 ggaggctcgg tcgcgacgcg ccgcgcgcga acctctacgg aaagctcgag tcggcgaacc
     1081 cgggcggctc ggtgaaggat cgcatttgcc tcgcgatgat cgagcgagcc gagcaagaag
     1141 gaaagctcgg gcccggcggc accgtggtcg aacccacgag cggcaacact ggcatcgggc
     1201 tcgcgctcgt gtgcgcggcg cgcggctatc gctgcgtgct caccatgccc gaaagcatga
     1261 gcctcgagcg tcgccagctg ctcgagtcgt tcggcgccaa gctcgtcctc accccggctc
     1321 aggagcagat ggagggtgcg atccgacgcg cgcgagagat cgtggccgag acgccgggcg
     1381 ccttcatgcc ccagcagttc gagaacagcg cgaaccccga ggtgcacgct cgcctcaccg
     1441 ctcgagagat catccacgcc atgggcggag agcgcgtcga cgcgttcgta gccgcggtcg
     1501 gcacgggagg aaccgtgagc ggcgtcggac gcgtcctgaa ggagacgttc ccgggttgcc
     1561 gagtgatcgc ggtcgagccc gagtcgtgcg cgacgatctc acgcggcgag cgcggaccaa
     1621 ccaagattca aggcatcgcc gccggcttcg taccgaagaa ctacgacccg agcgtgcccg
     1681 acgaggtgcg caccgtgagc gacgagcgcg cctaccagac caagcgagag cttgctcagc
     1741 gcgaagggct gctggtggga atcagcgccg gcgccaacgt cgccgtggcg ctcgatgtcg
     1801 cgcgggagct cggcgagggc aagacggtca tcaccgtgtt ctgcgatacg ggtgagcgct
     1861 acttcagcct cgacgagtac ttcaagccgt gagccgcgct cggcctctcc gctcgagtgc
     1921 gggcgtcctc caaggacgcc gggcgctcgt cgtcggcgca ggtggcctcg gaacgccagc
     1981 cctgctcgcc ctcgccgaca gcggtgcgac tctcgtcatc gccgatgacg acgtggtcga
     2041 gctcagcaat ctgcaccgac agatcctatt ttcggagagc gacgtaggtc gccacaagct
     2101 cgacgcggtc cgcgacgcgc tcgagaaaga gggtgtgagc cctacgcgca tcgagctcgt
     2161 ccgttcgcgc ttcttgcccg aaaacgcggt cgagctcgcc cgaggtgtgg acatcgtgct
     2221 cgaaggctcg gacaatttcg cgacgaagtt cctggccgcc gacgccgctc acctagccgg
     2281 tcgccccgtc gttcatggcg ccgcggtccg cctcgagggc accgtgtggt gcgtgccggc
     2341 gcgaggcgcg ccctgttacc gctgcttgtt cgaggacgtg cccggcggcg cgcagcaagg
     2401 gtgctcggag gccggagtgc tcgcgccggt ggtgggcatt tgcggcgcgt tgatggccga
     2461 gctcgccctg cgcgccttgt gcgggctcga cgcttacgag gggtcgctca tcgccgtgaa
     2521 cggcaggcga aaccgcctcc gccgcgtgcc ggtcgcggca cgccaaagct gtccgctttg
     2581 cggcgaggct cccagcattt tcgaaatagt agagacgcgc tacacagctc cgagctgtgc
     2641 tgcctgaacc ataaaggaga acaccatggc aaccgtccgc atccccaccc cgctacgcac
     2701 gctgaccaaa ggtgaagagc aagttcaagc cagcggcgac accgtccgcg ccgtcatcga
     2761 gacgctcgag aaagaccacc ccggcatccg cgaacgtctg ctcgacgaaa aaggcgttcg
     2821 ccgcttcgtg aacatctacg tcggcgatga ggacatccgc ttcctcgacg gcttggacac
     2881 ccagctgaag agcagcgacg aaatcagcat cgtccccgcg atcgccggcg gcgtgtgacg
     2941 tcatgaacga gcggctcgat cggcaggttc ggctccgcga agtcggtgag cgcgggcaag
     3001 cgaacatcga gcgctcgtac gcacgcgtcg aacgcggccc tgccgcgcgc atcgagctca
     3061 cctatttggt gcgggccggc gtcgaacggg cgagcatcgc cgagttcgat gagggcccgt
     3121 tcgcccacga atcttggttc gaattctccg ggccccgcaa cgtcgcgcgc ggggcgagcg
     3181 acgccctcga ccacctcttg agctgcttgg agctcccatg accgcacgtc ttcatcgttc
     3241 gcgaaagctc ggctcgatca tcgaggccgt tggaaacacg ccgctcttgc ggctcgaacg
     3301 cgttgggcgc tcggcgccga gcgtcgaggt ctacgtgaag ctcgagttcg cgaacccggg
     3361 cggctcggtc aagg
//
LOCUS       DASTKS010000026         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_449831, whole genome shotgun sequence.
ACCESSION   DASTKS010000026 DASTKS010000000
VERSION     DASTKS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449831"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFQ35_00565"
     CDS             <1..447
                     /locus_tag="VFQ35_00565"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET9929139.1"
                     /translation="FDAVCASIRHRYETDRPFAIVVVAEGAKPVGGAQIVARQELAKE
                     ATLGGVGAYVAQEIARRTGYETRSLVLGHLQRGGTPTAFDRVLALRYGAAAVRAAVEG
                     AWGHMVSYDPPHMRFVPIADAIAKMRSVPLDHDGFTTARELGICLG"
     gene            complement(503..958)
                     /locus_tag="VFQ35_00570"
     CDS             complement(503..958)
                     /locus_tag="VFQ35_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929140.1"
                     /translation="MKNLVVGLGFVLAVLLAPVRAEAKGIPIVYNTGQEAFEAGPLPP
                     PFDAVSELAGYKAGYLCDITGVLWSYFSVRNCKPVAFKDDSYADDAEIVKAISAKYTE
                     ADMKRGIWARFGWMLMAALVVLGAAVWVYEAVTGKSSDDEDDSTSTGQA"
     gene            complement(1369..>3446)
                     /locus_tag="VFQ35_00575"
     CDS             complement(1369..>3446)
                     /locus_tag="VFQ35_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9929141.1"
                     /translation="KWKFLPAEREGKPLAAKVRFLVRFTPPAVAPADEATPSAVPETT
                     TPSAPKGTPRVKRTGSEVSYEVVVVGTREPLRHQLGRAEVHDMPGAFGDPYRAIESLP
                     GVVPIVSGLPYFYVRGAPPGNVGYFFDGVPVPYLYHFAAGPGVLHPAFVDHVDLYPAA
                     YPARYGRFAGAIVAGEMAEPGYAFRGEASIRIIDSGAMLETPFADNRGSIMLGGRFSY
                     TGFVLSLIVPEVTLNYWDYQARIRYQLSNKDRIEVLTFGSRDFLSQKEREYTDSGATP
                     IERENTIVDVGFHRLDMRWDRRIEGGNLRTALLTGVDRTGADDGQVSVTNYLLGARSE
                     VEKTVSQGVLLRAGADVLFESLKQRVNQNDGRDCPEPQAPGEVPGNCFEDNDRDPNFN
                     FNSSRRDITAGLRADLVWDAAPRVQVVPGLRLDLYNSGDITRFAADPRLSVRYELSRK
                     LSVVHGLSLVHQAPSFVVPVPGFKPSLKGGLQRALQSSAGFTYKLPSDFTASLTVFQN
                     AFFNMTDFIGLAQLDQSGGGDLQDARSMGHAYGMEVMVRRSLSRDLGGFLSYTLSRSL
                     RTSGSAAGPATTDRTHVVNLGASYNLGRNWRLGGRVLFYSGIPSQVAYLAAAKSPPRT
                     PPFWRLDVKLQKRWYIVPNKSWWGLVFEVLNTSLNREVLNGDCNAYGCKYEDFGPVTV
                     PSIGAEGAF"
BASE COUNT          636 a         1131 c         1084 g          595 t
ORIGIN      
        1 ttcgacgcgg tgtgcgcgtc gattcggcat cgctacgaga cggatcgccc gttcgcgatc
       61 gtcgtcgtgg cggaaggcgc gaagccggta ggcggcgcgc aaatcgtcgc gcgccaagag
      121 ctcgcgaaag aggccaccct cggcggagtc ggcgcttacg tcgcacaaga gatcgcgcgg
      181 cgcaccggct acgagacgcg cagcctggtg ctcgggcacc tgcagcgcgg cggcaccccg
      241 accgcgttcg atcgcgtgct cgcgctgcgg tacggcgcgg cggcggtgcg agccgccgtg
      301 gaaggcgcgt ggggacacat ggtgagttac gaccctcccc acatgcgctt cgtcccgatc
      361 gccgatgcga tcgcgaaaat gcgctcggtc cccctcgacc acgacggctt caccaccgcg
      421 cgcgagctcg gaatttgtct gggctgatgc gccgcgcgct tccgcgctcg gcggcgacgc
      481 tcaccctata gggttcgagc cgtcacgcct ggcccgtcga cgtcgagtcg tcctcgtcat
      541 ccgaagactt ccccgtcacg gcctcgtaga cccacacggc ggcgccgagg acgaccagcg
      601 cggccatcaa catccacccg aagcgcgccc aaatcccgcg cttcatgtcg gcctcggtat
      661 acttcgcgga aattgccttc acgatctcgg cgtcgtccgc gtacgagtcg tccttgaaag
      721 cgaccggctt gcagttacgc accgagaagt aggaccacag tacgccggtg atgtcgcaca
      781 gatagcccgc cttgtacccg gcgagctcgc tcacggcgtc gaacggcggg ggcagcggcc
      841 cagcctcgaa cgcctcttgc cccgtgttgt aaacgatggg gatccccttc gcctcggcgc
      901 gcaccggtgc caacaaaaca gccaacacga accccagccc aaccaccaga ttcttcattt
      961 tctgcatatt accttgcggc gcgcgcgttc ttcccgaaaa atcttcgcta cccggcaagc
     1021 atgattcgag cttggaagtt catgagcgct ttcgctctct tcgtcctaac cgcctgccag
     1081 ggagaatacc cactcccacc tacgcgctgc gacgactggt gccacctgag cgaatcgacc
     1141 ggctgcagct tcttcagccc ttcgagttgt gttcgtgagt gcgaagcgca aggttttacg
     1201 accgactgtt ccgcagagtt cgacgtcgca ctccagtgtc tgaaagacca tccgcccaag
     1261 agctcggcgt gtggcttcct gtcgagtggg cagccggggc cgtgcgccac cgaacagggc
     1321 gaattacaga gctgcgccgg ctcgcacttc agagaatgag gggagcgctc agaaggctcc
     1381 ctcggccccg atgctcggaa ccgtgaccgg cccgaagtct tcgtatttac acccgtaggc
     1441 gttacaatcg ccgttcaaga cctctctgtt gaggctggtg ttgagcacct cgaatacgag
     1501 tccccaccag ctcttgttcg gcacgatgta ccagcgcttt tgcagcttca cgtccaagcg
     1561 ccaaaacggc ggcgtgcgcg gaggcgactt ggccgctgcg aggtacgcaa cttgcgaagg
     1621 gatccccgag tagaagagca cgcgaccgcc gaggcgccag ttgcggccca agttgtaaga
     1681 tgcgccgagg ttcacgacgt gagtgcgatc cgtcgtggct ggacctgccg cgctgccgga
     1741 agtgcggagt gagcgcgaga gcgtgtacga gagaaaccca ccgagatcgc gcgaaaggct
     1801 gcgacgtacc atcacttcca tgccgtaggc gtggcccatc gagcgcgcgt cttgcaggtc
     1861 tcctccgccg ctttgatcga gctgcgcgag cccgatgaag tcggtcatgt tgaagaaggc
     1921 gttttgaaaa acggtcagcg aagcggtgaa gtcggaagga agcttgtacg tgaaaccagc
     1981 gctcgattgc agggctcgct ggagtccgcc tttgagcgaa ggtttgaagc ccggcaccgg
     2041 cacgacgaag ctcggcgcct ggtgcaccaa cgacagcccg tgcacgaccg aaagcttgcg
     2101 gctgagctcg taacgaacgc tcaatcgtgg gtccgcggca aaccgggtga tatcgcccga
     2161 attgtagagg tctaggcgaa ggcccggcac cacttgtact cggggcgcgg cgtcccaaac
     2221 caagtccgcg cgcagcccag cggtgatgtc acgccgagag ctattgaagt tgaaattcgg
     2281 atcgcggtcg ttgtcctcga aacaattccc gggcacctct cccggagcct gcggctctgg
     2341 acaatctctt ccgtcgttct ggtttacgcg ctgcttcagc gactcgaaga gcacgtcggc
     2401 gccggcgcgc agcaggacgc cttggctcac ggtcttttcg acctcgctgc gcgcgccgag
     2461 caggtaattc gtgacgctga cctgcccgtc gtcggcgccg gtgcggtcga cgccggtgag
     2521 caacgcggtc cggaggtttc cgccttcgat gcggcgatcc caacgcatgt cgagccgatg
     2581 gaacccaacg tctacgatcg tgttctcgcg ctcgatcgga gtcgcgccgc tgtcggtgta
     2641 ctctcgctcc ttttgagaga ggaagtcgcg ggagccgaag gtcaagactt cgatgcgatc
     2701 cttgttcgac agctggtaac ggatgcgcgc ctggtagtcc cagtagttca gcgtgacttc
     2761 cggcacgatg agcgagagca cgaagccggt atacgagaac cggcctccca acatgatcga
     2821 gccgcgattg tcggcgaagg gcgtctccag catcgcgccg ctgtcgatga tgcgaatgct
     2881 ggcttcgccg cgaaacgcgt agccaggctc ggccatttcg cccgccacga tcgcgcccgc
     2941 aaagcgtccg tagcgcgccg gatacgccgc cgggtacaaa tcgacgtgat cgacgaacgc
     3001 cggatggagc acaccgggcc ccgccgcaaa gtgatagaga tacggcaccg gcacgccgtc
     3061 gaagaagtaa ccgacattcc cgggaggcgc gccgcgcacg taaaagtacg gcagcccgct
     3121 cacgatcggc acgacaccgg gcaacgactc gatcgcgcga tacggatcac cgaaagctcc
     3181 gggcatgtcg tgcacttcgg cgcgtccgag ctggtgtcgc agaggctcgc gcgtccccac
     3241 gaccacgact tcgtacgaaa cttcggagcc ggtacgcttc acgcgcggag tccccttggg
     3301 cgccgacggc gtcgtggtct cgggcacggc cgacggcgtc gcctcgtcgg cgggcgcgac
     3361 cgcgggagga gtgaaccgca cgaggaagcg caccttcgcc gcgagcggct tgccttcgcg
     3421 ttcggcaggc aaaaacttcc acttct
//
LOCUS       DASTKS010000027         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1118918, whole genome shotgun sequence.
ACCESSION   DASTKS010000027 DASTKS010000000
VERSION     DASTKS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118918"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="VFQ35_00580"
     CDS             complement(<1..2126)
                     /locus_tag="VFQ35_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9929142.1"
                     /translation="MATAVPPAVSAGSAELLGKSLLSATEEAQIASEALLRQFIKHTP
                     AAVAMFDCEVRYLQASDRWITDYRLEGRDLVGRSHYEVFPDIPLRWKEVHCRVLKGAV
                     ERCAEDPFPRADGSVEWLEWEVRPWFKADGTVGGLIMFTQAITERKRIAEALRASEER
                     LKSAMTHSPIGMAVVAPDGRFLEVNPALAKLLGYSPQELLARDFQSITHPDDLEADLD
                     LLRKTVEARSEGYQLEKRYLHRDGRAIWIQLNASIVFDERGKPRHFIAQIQDISERKR
                     SERRQRRLLETPILGIAFGQPDGRITAANDEYLRIVGYSRDELEAGKINFREVTPAEY
                     APQNELALAEIEAKGTARPWELELTRKDGARIPVLLGVSALDDDHSQDVAFVFDLSER
                     RDLERKLQHAQKMEAVGRLAAGVAHDFNNVLGAVSMCCDLLRDDLSPGHPGLDEVNEI
                     RKAAERAIGLTRQLLAFSRQQVLEPRVLDLNELVTNLDKLLKRLLGDDVALAMNLAPF
                     PCAVKADPGQLEQVIMNLAVNARDAMPHGGTITIETGVSHFEQPYTADNEDALPPGAY
                     VHVTVADTGIGMDEATKARLFEPFFTTKEVGKGTGLGLSTVYGIVRQSGGAIDVKSEV
                     GAGARFRVMLPHVNESVKSTPPTGLPESIGGRETLLLVEDDPAIRQTARKLLVRLGYK
                     VLESDGPAAALELGRVYVGSIDLLLT"
BASE COUNT          329 a          694 c          716 g          412 t
ORIGIN      
        1 tccgtgagga gcaaatcgat cgagccgacg tacacacgtc cgagctcgag tgcggcagcg
       61 ggcccatccg actcgagcac cttgtacccg agacgcacca agagcttgcg cgccgtttgg
      121 cggatcgcgg ggtcgtcttc gaccagcaac agcgtttctc gcccgccgat cgactcgggc
      181 agcccggtcg gcggcgtcga cttcacgctt tcgttcacgt gcggcagcat gacgcggaag
      241 cgcgctcccg cgccgacttc gctcttcacg tcgatcgcgc cgccgctctg gcgcacgatg
      301 ccgtagacgg tcgaaaggcc gagcccggtg cctttgccga cttccttggt cgtgaagaac
      361 ggttcgaaca agcgagcctt ggttgcctcg tccatcccga tgcccgtgtc ggcgaccgtg
      421 acgtgcacgt aggctcccgg cggcagcgcg tcttcgttgt cggccgtgta gggttgctcg
      481 aagtgcgaaa cgccggtctc gatggtgatc gttccaccgt gcggcatcgc gtcacgcgcg
      541 ttcacggcca agttcatgat cacttgctcg agttgcccgg ggtcggcctt caccgcgcat
      601 ggaaacggcg cgaggttcat ggcgagggcc acgtcgtcgc cgagcaggcg cttcagcagc
      661 ttgtccaggt tcgtgacgag ctcgttcaga tcgagcacgc gcggctcgag tacctgctgc
      721 cgactgaagg cgagtagctg acgggtgagc ccgatggcac gctcggcggc cttgcggatt
      781 tcattcacct cgtcgagccc aggatggccc ggggaaaggt cgtcgcgcag gagatcgcag
      841 cacatggaca ccgcgccgag cacgttgttg aagtcgtgag cgaccccggc cgcgagtcgc
      901 cccactgcct ccattttctg cgcgtgctgg agcttgcgct cgaggtcacg tcgctcgctc
      961 aagtcgaaca cgaaggcgac gtcttgactg tggtcgtcgt cgagcgccga gacgccgagc
     1021 agtacgggga tgcgcgcgcc gtccttgcgg gtgagctcga gctcccaggg gcgcgcggtg
     1081 ccctttgcct cgatttcggc gagcgcgagc tcattctgtg gcgcgtactc ggcgggcgtg
     1141 acctcgcgga agttgatctt tccggcctcg agctcgtcgc gcgagtagcc gacgatgcgc
     1201 aggtactcgt cgtttgcagc ggtgatgcgt ccgtcgggct gaccgaaggc gatgcccaag
     1261 atcggtgtct cgagcaggcg gcgttggcgc ctctcgctgc gtttacgttc gctgatgtcc
     1321 tgaatttgcg cgatgaagtg gcggggcttg ccgcgctcgt cgaacacgat cgaagcgttg
     1381 agctgaatcc agatcgcgcg tccgtcacgg tgcaggtagc gcttctccag ctggtagcct
     1441 tcgctgcgcg cctccacagt cttccgcaac aaatcgaggt cggcctccag gtcgtcgggg
     1501 tgcgtgatgc tctggaagtc tctggcgagg agctcttggg ggctatagcc gaggagcttc
     1561 gcgagcgcgg ggttcacctc gagaaagcgg ccgtccggcg ccacgaccgc catgccgatt
     1621 ggcgagtgcg tcatcgcgga tttcaagcgc tcttcgctcg cgcgcagcgc ttcggcgatg
     1681 cgtttgcgct ccgtgatcgc ctgcgtgaac atgatgagcc cgccgaccgt gccgtcggct
     1741 ttgaaccacg gccgaacctc ccattcgagc cactcgacgc tgccgtcggc gcgggggaac
     1801 ggatcctccg cgcagcgctc gaccgcgcct ttgagcacgc ggcaatgcac ttctttccag
     1861 cgcaggggga tatcggggaa gacctcgtag tgcgagcgcc cgaccaagtc gcgcccctcg
     1921 aggcgataat cggtgatcca acgatcgctc gcttggagat accggacctc gcagtcgaac
     1981 atcgcgacgg cggccggcgt gtgcttgatg aactggcgaa gcagcgcttc gctcgcaatc
     2041 tgcgcctctt ctgtggccga gagcagactt ttccccagga gctctgcgct ccccgcagac
     2101 accgcgggtg gcactgccgt ggccatggtt cagcgctggg tcaccagttg t
//
LOCUS       DASTKS010000028         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_226662, whole genome shotgun sequence.
ACCESSION   DASTKS010000028 DASTKS010000000
VERSION     DASTKS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226662"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFQ35_00585"
     CDS             complement(<1..811)
                     /locus_tag="VFQ35_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929143.1"
                     /translation="MTKTFASFASFSLAFSLFACGSGGEPAQNSGAGGSTAGTSGARG
                     GSANATGGATTASSGGSTASAGGNTNGTQGGSSSTSMGGASNGSGGSSAGGSGSAQGG
                     ATSGGVGGASASGGQSQSGGAAQGGGTASGGGMATGGDEPKLITSNEKSFWQTATPTE
                     VTSGTADVTVNDTAAQTFTGFGGAFNEMSWDALSVLSEADRTRALKLLFDAQDGANFI
                     YGRIPIGANDYSTIADGPYTLADTANDTAMANFSIARDQKRIIPFIKAAKAV"
     gene            complement(1236..2009)
                     /locus_tag="VFQ35_00590"
     CDS             complement(1236..2009)
                     /locus_tag="VFQ35_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929144.1"
                     /translation="MKASSVLGILLVGVCAGCSAANEEDVGSLDQAAKQTDFGVDFGS
                     CTEVAAIGNVPRANVRPLVPAKYDLAGDAQNAVLVARAVNCGAVSVDGKAARPTTLIQ
                     IGVSLVGAAVANPVADINNYTLWYVTDNSQLFAKLNSAGANAENSQHLSYTVTSGVFD
                     FSASPARAPAVNLHGPVTPGIFPPQLFRATWWSDGNHGTIRMHTPFPQIRFGVATTTL
                     TTPANSDLSRVIGGTSMTFSLLDSSNTFDSARMEVVSVP"
     gene            complement(2059..>2684)
                     /locus_tag="VFQ35_00595"
     CDS             complement(2059..>2684)
                     /locus_tag="VFQ35_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929145.1"
                     /translation="ENALPHVFEPFYTAKRRGKGSGLGLPISKRIIDSLGGTMSIQSV
                     LGRGTAVFIELDQAPPTLAEEGSAVAERVVERRVTPRARVLVVDDERPLARTLRRLLS
                     AHDVVLAYDGSAAYDILQSDTRFDVILCDLMMPGLSGADLYHRVSALHPELESRFIFM
                     TGGSMSELGDAFVESVRSRMLTKPFDPDRMLEWVETAARRASEITVK"
BASE COUNT          486 a          889 c          828 g          481 t
ORIGIN      
        1 tgaccgcctt cgcggccttg atgaacggga tgatccgctt ctggtcgcgc gcgatactga
       61 agttcgccat cgcggtgtcg ttggcggtgt cggcgagcgt gtagggacca tccgcgatcg
      121 tgctgtagtc gttcgctcca atcgggatgc gtccgtagat gaagttcgcg ccgtcttgcg
      181 cgtcgaacaa gagctttagc gctctcgtgc ggtcggcctc gctgagtacg gagagcgcat
      241 cccaactcat ttcgttgaac gcgccgccga agcccgtgaa ggtttgggcc gcggtatcgt
      301 tcacggtcac gtctgcggta ccgctcgtca cctcggtggg cgtcgccgtc tgccaaaacg
      361 acttctcgtt ggacgtaatg agcttcggct cgtctccgcc cgtcgccatg cctccacccg
      421 acgccgttcc gccgccctgc gccgctccac cgctctggct ctgcccgccg ctagcgctcg
      481 cgccgccaac tccaccagac gtggcgcccc cctgagccga gccactcccg ccggcagagc
      541 tcccgcccga accgttcgac gctccgccca tgctcgtcga ggagcttccg ccctgagttc
      601 cgttcgtatt tccgccagcg ctcgccgtgc tcccgccgga cgaagccgtg gtggcgccgc
      661 ccgtagcgtt cgcactgccg ccgcgcgcac ccgaggtccc tgcagtcgac cctccggcac
      721 cggagttttg agcaggctcg ccgccactgc cgcaagcgaa gagagagaaa gcgagtgaga
      781 agctagcgaa cgaagcgaat gtcttggtca tggcgagtga gggcaagatc tgcccccacc
      841 ctattcccag ttcgactccg accgatcagc gcagagtcga actctggcct cgatcggcgc
      901 tgcgcaacgc gacacgactg caaaagttcg acgtgcccgc ccaaacgatg agcgcgctta
      961 gcagggcgtt gaaaaacgcc ccgtcacgac agaccaatgc caggcacaca cgacgcgcag
     1021 aagcaagcat cacgaaagcc tcacattcgc tttggtttgt gtggttttcg gggccccggg
     1081 aaccccgaaa acggcgggcc gaggcgtggt tcggtgatcc gataaatcct gagactaaag
     1141 taggcggggg agcgcagcga cgagcccgtc cgccgcagca agaggatagg ctcgcgcagc
     1201 tatgacttat ccattcgagg gcccttagca ggctgttaag gaacgctcac gacttccatc
     1261 cgcgccgagt cgaaagtgtt ggagctgtcg agcaacgaga aggtcatgga tgtaccgccg
     1321 atgacgcgcg aaaggtcgct gttcgccggt gtcgtcagcg tcgtggtcgc gacgccgaag
     1381 cgaatttgcg gaaacggcgt gtgcatgcgg atcgtgccgt ggtttccatc cgaccaccac
     1441 gtggccctga agagttgagg agggaatatc cccggcgtaa ccgggccatg gaggttcacc
     1501 gcaggagctc gcgcggggct cgcgctgaag tcgaacacgc cggacgtgac cgtgtacgag
     1561 aggtgctggg agttctctgc gttcgcgccc gcggagttca gcttcgcgaa gagctgtgag
     1621 ttgtcggtca cgtaccacag agtgtagttg ttgatgtcag cgaccgggtt tgccaccgcg
     1681 gccccgacga gcgagacccc gatttggatc agggtcgttg gccgcgccgc tttcccatcg
     1741 accgacaccg ctccgcagtt gacggcgcgc gcgacgagca cggcgttctg cgcgtcaccc
     1801 gccaaatcgt acttggcggg gacgagcgga cgcacgttgg ctctgggaac gttgccgata
     1861 gccgccacct ccgtgcacga gccgaaatcg acaccgaaat cggtctgctt cgcggcctga
     1921 tcgaggctgc caacgtcttc ctcgttcgcg gcgctgcagc cagcgcaaac ccccaccaac
     1981 aaaatcccta gcaccgagct tgccttcatt tcggaccctc cattcatgtt cgaactcgcg
     2041 acgaaggagc agcccgagtc acttcaccgt gatctcactc gccctccgag ccgccgtttc
     2101 gacccactct aacatacggt ccggatcgaa cggcttcgtc agcattcggc tccgcacact
     2161 ttcgacgaac gcatcgccga gctcgctcat gctcccaccg gtcatgaaga tgaagcgcga
     2221 ttcgagctcc ggatggagcg cgctgacacg gtggtacaaa tccgcacccg agaggcctgg
     2281 catcatcaaa tcgcacagaa tgacgtcgaa gcgcgtatca ctctgtagga tatcgtaagc
     2341 ggcactccca tcgtaggcga ggacgacgtc gtgtgcgctg agcaagcgac gcagcgtgcg
     2401 cgcgagcggg cgctcgtcat cgacgacgag gacgcgtgcg cgcggcgtca cgcgtctctc
     2461 gacgacgcgc tcggcgactg cgcttccctc ttccgcgagc gtcggcggcg cctgatcgag
     2521 ctcgatgaat acggcggttc cgcgaccgag cacgctctgg atgctcatcg tgccgccgag
     2581 cgagtcgatg atgcgcttcg aaatgggcag cccgaggccc gaccctttgc cacgccgctt
     2641 cgcggtgtaa aacggctcga acacatgggg cagcgcgttc tcgg
//
LOCUS       DASTKS010000029         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1119174, whole genome shotgun sequence.
ACCESSION   DASTKS010000029 DASTKS010000000
VERSION     DASTKS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119174"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1393)
                     /locus_tag="VFQ35_00600"
     CDS             complement(146..1393)
                     /locus_tag="VFQ35_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9929146.1"
                     /translation="MAELTREEVLQRAQARQSFARVDLRGLDLSQANLEGLDFGRAEL
                     DGVNLSGAKLKGASFRNASLREAFLKQADLREANLDKADLEGANLEGADLSLANLNRA
                     TLEGAKLTQALVRGARLTGAQLDSSDLGKADLEGATLAHAELNDCYLGGANLKSAELA
                     NAVLSQANLEEANLEKANLREAELDAVKAPKANFSGALLVRANLSNARFGGANLEGSD
                     LRHANLNGADLTGALLTGAKVNGAATQNAKWSSPAADWLDASPGGDGTRQVRGDDIDA
                     LLSGKLAAPSRAARYFGKGDVLRDASLEFGDGSSIEIESRFENCSITLGNGTDLVIGE
                     AGVLSQCRIQGGGNITILGQFFERESPGIRGPKRLVVTGTGAVVGSVEQASGATEFAF
                     QPGCRLRMKILKARIGTERSGSE"
     gene            complement(1425..2552)
                     /locus_tag="VFQ35_00605"
     CDS             complement(1425..2552)
                     /locus_tag="VFQ35_00605"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9929147.1"
                     /translation="MPGPIRPAPSKPAPGEPPSSISEALADVAAGAPESALVDEKRAF
                     SQGAPRPPEPPPLLTAEKTPLPGTSKPAPNPTLDEIDKRFETIISEPPGPGQELTPEE
                     LREAQRLFLEISVNYLRPLRDLIVEIDLGEPTREWLSVCRPAVASLRRAAMDMALEDL
                     GADLGGLLTVIEQGERGIGSTLDARTREALKAAWGKLAVTLPQAFSVEQTRTRREPLI
                     VFSLLQQVPDVRKVTLDRIVAAGLTTLEQLYKAKPGDIADAAGIPKELAERIVARFQR
                     YRRELGTAPPSPSRQREKAQLDALAKKLEDQNNAFDASSRHWSHAEEKRRLRQERAAT
                     LLEIRLLLARLGEVELIDELEPLPFQKKAETLRRYLSAQNE"
     gene            complement(2968..>3252)
                     /locus_tag="VFQ35_00610"
     CDS             complement(2968..>3252)
                     /locus_tag="VFQ35_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9929148.1"
                     /translation="GELEALDEQAWVERATRGLERIFPLRAAPLHAWVSRWSHALPVS
                     NPAHSERVRHLEAALHGSRILLAGSAFHGSGIDAAVRSAQQTAERLRALV"
BASE COUNT          482 a         1080 c         1092 g          598 t
ORIGIN      
        1 tgcacggaac ccttttcgct caccgagagt gcaggcgtgt cgatttcgcc ttcgattctc
       61 ccgcggacga cgatggggca ggtcgagagc agggtgcctc gaaactcagt gccctcttcg
      121 acgatggtct gcttggcatt cgtgctcatt cgctacctga cctttcagtg cctatgcgcg
      181 ccttcaaaat cttcatgcgt agccggcatc ccggctggaa cgcaaactca gtagcccccg
      241 aggcttgttc gaccgagccc accaccgcgc ctgtaccagt caccacgagc cgcttggggc
      301 cacggatgcc ggggctctcg cgctcgaaga actgcccgag gatcgtgatg ttgcctccac
      361 cttgaatccg gcattggctc aagactcccg cctcgccaat gacgagatcg gtgccgttgc
      421 ccagggtgat ggagcagttt tcgaaccggc tctcgatttc gatactggag ccgtcgccga
      481 actcgagcga agcgtcgcgc aaaacgtcgc ctttgccgaa atatcgggcc gcgcgcgacg
      541 gagccgcgag tttgccggaa agcagcgcgt cgatgtcgtc accgcgcact tgccgggtcc
      601 cgtcgccgcc ggggctcgcg tcgagccaat ccgcggcagg cgagctccac tttgcgtttt
      661 gggtcgcggc gccgtttacc ttcgctcccg tgagcagggc gcccgtcaga tccgcgccgt
      721 tcaggttcgc gtgccgcaga tccgagccct cgaggttcgc gccgccgaag cgcgcgttgc
      781 tcagattggc gcgcacgagc agggcgccgc tgaagtttgc tttgggggct ttaaccgcgt
      841 cgagctcggc ttcgcggaga ttcgcctttt cgaggttcgc ttcttcgagg ttcgcttgcg
      901 aaagcacggc gttcgcgagc tcggcgctct tcaagttcgc tccgccgagg tagcaatcgt
      961 tgagctcggc atgggcgagg gttgcgccct cgaggtccgc tttgccgaga tccgaggagt
     1021 cgagctgtgc accggtgagt cgcgccccgc gcacgagcgc ttgcgtgagc ttcgctccct
     1081 cgagggtcgc tcgattcagg ttcgccaggc tgagatcggc gccttcgagg tttgcgcctt
     1141 cgagatccgc tttgtcgagg ttcgcctcgc gcaggtcggc ttgcttcaag aacgcttcgc
     1201 gtaggctcgc gttgcggaag ctcgcgccct tgagcttggc gcccgacaag ttcacgccgt
     1261 cgagctcggc tcgcccgaag tccaagccct cgaggttcgc ttgggacaag tcgagccccc
     1321 gcaagtcgac gcgcgcgaag ctttgcctcg cttgcgcgcg ctgcaacacc tcttcccgag
     1381 tcagctccgc catctagtaa gtcctccggt tttgcgctcg gtcgtcattc gttttgcgcc
     1441 gaaagatagc gcctcaaggt ttcggccttc ttttggaagg gcagcggctc gagctcgtcg
     1501 atgagctcga cttcgccgag ccgcgcgagc aaaagcctga tttcgagcag cgtcgccgct
     1561 cgctcctgcc gcagccttcg cttctcctcg gcgtgcgacc agtgccgcga gctcgcgtcg
     1621 aacgcgttgt tttggtcttc gagcttcttc gcgagcgcgt cgagctgagc cttttcgcgt
     1681 tggcggctcg gcgacggtgg ggcggtgccg agctcgcgcc gatagcgctg gaagcgggcc
     1741 acgatgcgct ccgcgagctc cttcgggatc ccggccgcat cggcgatgtc accgggcttg
     1801 gccttgtaga gctgctccag cgtcgtgagc cccgccgcca cgatgcgatc gagcgtgacc
     1861 ttacgcacgt cgggcacttg ctgcaacagc gagaaaacga tcagcggctc gcgccgggtg
     1921 cgcgtctgtt ccaccgaaaa cgcctgcggc aacgtgaccg cgagcttccc ccacgcggcc
     1981 ttcaaagcct cgcgggtacg cgcgtcgagg gtgctgccga tcccgcgctc cccttgctcg
     2041 atcaccgtga gcaacccgcc gagatcggca ccgagatctt cgagggccat gtccatcgcg
     2101 gcgcgtcgca acgacgccac ggcagggcga cacaccgaaa gccattcacg ggtcggctca
     2161 ccgagatcga tctcgacgat gagatcacgc aagggtcgca ggtagttgac cgagatctcg
     2221 aggaacagcc gttgtgcttc tcgcagctct tccggggtta gctcttggcc gggaccgggt
     2281 ggctcggaga tgatggtctc gaagcgcttg tcgatctcgt cgagggtcgg gttcggcgcg
     2341 ggcttcgatg tgccggggag cggggtcttc tcggccgtga gcaggggcgg aggctccggc
     2401 ggccgcggcg cgccctgcga aaaagcgcgc ttctcgtcca ctagcgcgct ctcgggtgct
     2461 cctgccgcga cgtcggcgag cgcctcgctg atggagctcg ggggttcacc tggcgccggc
     2521 ttcgaaggcg ctggtcgaat ggggcccggc aaggtcgacc ggcgcggagc cggcggacga
     2581 cggggtgcgg acgaattgaa agccggtgcg ggcgcggacg ggcgcggtgc catgggcgcg
     2641 gtgctgtgcg aggcgcgcgg gatctgcact ggggcttgtg gactcccgac cgggctgctc
     2701 ggtcgcgccg tttccacggg cggggcgggc tccaccacgg gcatgcgtcc gccgcattcg
     2761 acgcaaaatg cggctccctc gagtccgagc gcgccacaat ggggacatcg tgcggagagg
     2821 cccgccatgg tgggcacgtt actaccatca tccccgcgga agcgcgcgcg cgaaatgacc
     2881 tctcgctgcg actcggtgag tgcgcttgga ctcgtggccg gccctcacgg cgtctcccgc
     2941 gagcggccca tggggcgtga gaccctttca gaccaacgcg cgaagtcgct ccgccgtttg
     3001 ctgggcggag cgcacggcgg cgtcgatacc cgagccgtgg aacgccgagc ccgcgagcag
     3061 gatgcgcgac ccatggagag cggcttcgag atgccggacg cgctccgaat gcgccggatt
     3121 cgaaaccggc aacgcatgac tccagcgcga gacccaggcg tgcaacggcg ccgcgcgcag
     3181 cggaaaaatt cgctcgaggc cgcgcgtcgc ccgctcgacc caggcctgct cgtccagagc
     3241 ttcgagctcg cc
//
LOCUS       DASTKS010000030         5015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1565182, whole genome shotgun sequence.
ACCESSION   DASTKS010000030 DASTKS010000000
VERSION     DASTKS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565182"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2403)
                     /locus_tag="VFQ35_00615"
     CDS             complement(<1..2403)
                     /locus_tag="VFQ35_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929149.1"
                     /translation="MVGAAFDIESYPKTFNTSSPASVKFACGTSRCLGVHRMTLDSGA
                     WVLATRVEAGGRVVDFPRIQVAQDAFPLRVLARKDEFLVITAPTETGDTGPLGYFLVR
                     GSDGVVTPVTSQGLPSTRPVLGASTGESWVLVWREGSGARGQVFDAQLVPRGPGAALE
                     AATLGMMADPIPGPGQYLFGAYGNAIRLDEASGNPLDATPIVVSSLFQPPRMAGVYSD
                     GVYQLAFGTQTIYQTRVEAATGKVLEPDDLFNQVLGPKLIATLPARATGDVDVDLVAG
                     KVVVSEAMVSDRAGAVVVDPKTGLAAGNNSIYAVLQNSPLGDPTHEKHVLGDSVFLLD
                     TGNLLQIGSGTQPLSPSFPSAKLALGISGIDRFGCRLAASKAGYLVVFARGSSFAQQG
                     SEIVATRIDPTTGAFLDDPPLVVGTGENPSVASDGQNYLVSWLSTADARTHAHYRLVK
                     ADGSMTQDNSSQIFDSVQSFAPSLTFDGLYYGVSWGSRAARIDASGTFVAPAQTPSDG
                     VFYNTGLIPTAGDPSARVAADTNQPPDHRTFLLAGETWVGASDSRNIGIVGIRSGTGA
                     MISGGILAEKHRRPFSVSDGTRVLVLSQEEASGSWYGVFVDPVSVAAIPNTRKLMIEN
                     PTPTNPSVYVQALSFDGSSYYVVVSERLDSSTLRSTTHLRRFDGSLARLAEESGPGIS
                     LDGQFQPTWYMDATTFGVDHGLFVFRTSDVARLGFAIKGVFFTADGTQPTTPSTGGVA
                     NGGGAANGGGGTGNSAGGVSNGSGGKTASAGNSSAGAGAGSPAGGSAQTTAGGTGQTT
                     AGG"
     gene            complement(2722..3333)
                     /locus_tag="VFQ35_00620"
     CDS             complement(2722..3333)
                     /locus_tag="VFQ35_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9929150.1"
                     /translation="MVTLYDFLDSGNGYKVRLTLRELAQPFVYQEVNIMRGETREDWF
                     LAKNPAGEIPVLELDDGTCLCESTAILFHLAEGTHLLPIDRLLRTRVLQWMCFEQTHV
                     DGVISRARFRRLYPDAIPTRAEEFEAWWAEGTRTLRVIEQQLVSREYLVGGSYSIADI
                     CAFAYVHRAPEGGFDMRPYPSIAAWCERIQSRAAFIALDRVPT"
     gene            3742..>5015
                     /locus_tag="VFQ35_00625"
     CDS             3742..>5015
                     /locus_tag="VFQ35_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9929151.1"
                     /translation="MDHIEHVETAETGCVSAPAAALTVLFCDDEPDNLELFLQQFGRT
                     YRVRTASSAAEALTVLAREDVALVITDERMPQTSGIELLNKVVEQWPDAVRVIVSAYG
                     DAGRLLRAINHGHAHEYVLKPWDRTELAACIERCLAIARRRRDLVMRAARAGLAETEA
                     RAEYAAEQVVGAESGLKCALSLARRVARTDATVLVRGETGTGKEMVARLIHEHSARKD
                     GPFVRVNCAALAEGVLESELFGHEKGAFTGANSLRRGRFELACGGTIFLDEIGDVSPK
                     LQSSLLRVLQEREIERVGASAPVPVNVRVITATHRDLEGRVREGTFREDLFYRINVVP
                     IVVPPVRARPEDVGPLVRHFLVKHAPPGRPPAFVSDATIARLSEYAWPGNVREIENLV
                     QRAVILAVGSEITLEDFCLRLDIPEDLPVREAV"
BASE COUNT          899 a         1587 c         1643 g          886 t
ORIGIN      
        1 gccacccgcc gtggtttgcc cggtgccgcc ggcggtcgtt tgcgcgctgc cgcctgcggg
       61 ggagccagcg ccagcgccag cgctcgagtt gcccgcagaa gcggtcttac cgccgcttcc
      121 gttgctgact ccacctgcag agttgccagt gccgccgccc ccgtttgctg ctccgccgcc
      181 gtttgctact ccgcccgtgc tgggcgtcgt cggctgagtc ccatccgcag tgaagaaaac
      241 acccttgatg gcgaagccca gccgcgcgac gtcgctggtc cgaaacacga acagcccatg
      301 atcgacgccg aaggtggtgg cgtccatgta ccaagtcggc tggaactgcc catcgagcga
      361 gattccaggg ccgctctctt ccgccagtcg agcgaggctc ccgtcgaaac ggcgcaggtg
      421 cgtggtggat cggagagtcg atgaatccag gcgttcactg acgactacgt agtaactgct
      481 tccgtcgaac gacaacgcct gcacgtacac gcttgggttc gtgggggtgg ggttttcgat
      541 catcaacttg cgggtgttcg gaatggcagc gacgctcact ggatccacga agacgccgta
      601 ccaagagccg ctcgcttctt cttggctgag gacgagcacg cgcgtgccgt cgctgaccga
      661 aaatgggcga cggtgcttct cggccaagat gccgccggag atcattgcgc cggtgcccga
      721 acggatgccg acgatgccga tgttgcgcga gtcgctagct ccgacccacg tctcgccggc
      781 gagcaagaac gtgcgatgat ccggtggttg attggtatcg gcggcgacgc gcgcgctcgg
      841 gtcgcctgcg gtcgggatta ggcccgtgtt gtagaaaact ccgtcgctgg gggtctgggc
      901 cggcgccacg aacgtccccg atgcgtcgat gcgcgcagca cgcgagcccc agctcacgcc
      961 atagtagaga ccgtcgaacg tgaggctcgg cgcaaagctc tgcaccgaat cgaagatttg
     1021 cgacgagttg tcttgggtca tgctgccgtc cgcctttacc aagcggtagt gcgcgtgcgt
     1081 gcgcgcgtcg gcggtcgaca gccacgacac cagatagttc tgcccgtcag acgcgacaga
     1141 aggattctca ccggtgccca cgaccaaggg cgggtcgtcc aagaaagcgc ccgtcgtcgg
     1201 gtcgatgcgc gtcgcgacga tctccgagcc ttgttgagcg aacgaggagc cgcgcgcgaa
     1261 gacgacgagg tagcccgcct tgctcgcggc gagcctgcac ccgaaccggt cgatgccgct
     1321 gattcccaac gcgagcttgg cagaggggaa gctcgggctg agcggctgcg tgccgctccc
     1381 aatctgcagc agattgccgg tgtcgagcaa gaacacggag tcgcccaaga cgtgcttttc
     1441 gtgggtcgga tcaccgagcg gtgagttctg caacacggcg tagatagagt tgtttcccgc
     1501 ggcgagcccg gtcttgggat cgacgacgac ggcgccagcg cgatcactca ccatcgcctc
     1561 gctcacgacc accttaccgg cgacgagatc gacgtccacg tcgccggtgg cgcgcgctgg
     1621 cagcgtcgcg atgagctttg ggcccagcac ctgattgaac aggtcatcgg gctcgagcac
     1681 tttgccggtc gcggcttcta cgcgggtctg gtagatcgtc tgtgtgccaa aggcgagctg
     1741 gtacactccg tcgctgtaca cgcccgccat gcgcggcggc tgaaataggc tcgagaccac
     1801 gatgggcgtc gcgtcgagcg gattgccgct ggcttcgtcg agccgaatcg cgttgccgta
     1861 cgcgccgaac agatactgtc cagggccggg aatcgggtca gccatcatgc cgagggtggc
     1921 cgcttcgagc gcggcgccgg gtccgcgcgg cacgagttgc gcgtcgaata cttgaccgcg
     1981 cgccccggaa ccttcgcgcc aaacgagcac ccaagattcg ccggtggacg cgccgagaac
     2041 gggtcgcgtc gagggcaagc cctgtgaagt tactggcgtg acgacgccgt cgctgccccg
     2101 taccaagaaa taaccgagcg ggccggtgtc gccggtttcg gtgggcgccg taatgaccaa
     2161 gaactcgtcc ttgcgcgcga gtacgcgcag cggaaacgca tcctgtgcga cctgaatgcg
     2221 cggaaagtcg acgacgcgac caccggcttc gacccgagtt gccaacaccc acgcgccgct
     2281 gtcgagcgtc atgcgatgga ctccgaggca gcgcgacgtg ccacacgcaa acttcacgga
     2341 ggcgggtgag ctcgtgttga aggtcttcgg atagctctcg atgtcgaacg cagcgccgac
     2401 catcactgcc agtgccgcac tttgtctcga ggcacttggc tccccgccat gaccgcagcc
     2461 gaacagcagc gccgctgccg tgcccaaccc gcaccaagtc ttcatgctcg ccgtagtctg
     2521 aatggatctg gccattcgcg gcaatgctga gcaagccaag tgccagtaca gcgacccctc
     2581 gcctatctca caactccgcg aattcacgct tgcgcggcgt tcgaggagga cgaccggttt
     2641 cgaggccgaa cgtccgcgtg gacgtccagt ttcgttgcgc cgccagtggt ggttggacgg
     2701 actcgtgcct cgcaccttcc gttaggttgg aacccgatcg agcgcgatga acgccgctcg
     2761 cgactgaatg cgctcgcacc atgcggcaat cgacggatac ggtcgcatgt cgaagccccc
     2821 ctcgggagca cggtgaacgt aagcgaatgc gcagatgtcc gcgatcgagt atgaacctcc
     2881 aacgagatat tcgcggctga ccaactgctg ctcgatcact cggagcgtgc gcgtgccttc
     2941 cgcccaccac gcctcgaatt cctcggcgcg agtcgggatg gcatcgggat acaagcggcg
     3001 gaacctagct cgtgagatga cgccgtccac gtgggtctgc tcgaagcaca tccattgcaa
     3061 gacgcgcgtc cggaggagcc gatcaattgg cagcaagtgc gtcccttccg ccagatggaa
     3121 taggatggca gtgctttcgc aaaggcaggt gccgtcatcg agctcgagga cggggatctc
     3181 accggcgggg ttcttcgcaa gaaaccagtc ttcgcgcgtc tcgccacgca tgatgttcac
     3241 ctcctggtac acgaagggct gagcaagctc gcggagggtg agtcgaacct tgtagccgtt
     3301 cccggaatcg aggaagtcgt agagcgtgac catgacgcgc ttggagtcct agttgtgatg
     3361 cgtgcgtggc gccactgcca atgcacggcg gtgtttcacg acaggtctcc acggcgagca
     3421 gaacgtcgcc acctgaagca gtctcgatgt cgaaggaact cctactggct gcggctccga
     3481 gcgctcgtct cgacgaagac ctgcgcggcg ccgttcatct cgttgcgttc tcctcgaatc
     3541 ctcgcgcaaa tcaccgcccg caccgagtga tcgcgactcg acgcgaatgc tgcgggttcg
     3601 tttcagctcg ctcggcagat tgctcatcgc ccttcgtctg gcggaatgac catggaatga
     3661 caacgacgag cgatgtaaac ccgcggtagg cagttaatct caaggggaca gtgcaagcga
     3721 tctcacatcg taggcagggg aatggaccac atcgagcacg tcgagactgc ggaaactggc
     3781 tgtgtctctg ccccggcggc agcgctcacg gttctgttct gcgacgatga gccggacaat
     3841 ctcgagctct tcctgcagca gttcgggcgc acctaccgcg tgcgtaccgc atcgagcgca
     3901 gccgaggcgt tgaccgtgct cgcgcgggaa gacgtcgcgc tcgtcattac ggatgagcgt
     3961 atgccccaaa cgagcggcat cgagctcttg aacaaagtgg tcgagcaatg gcccgacgcg
     4021 gtgcgcgtca tcgtgtcggc gtacggtgac gcggggcgtc tcttgcgcgc gatcaatcac
     4081 ggtcacgccc acgagtacgt cctgaagccc tgggaccgaa ccgagctcgc cgcctgcatc
     4141 gagcgctgct tggcaatcgc tcggcggcgg cgggacctcg tgatgcgcgc cgcgcgcgca
     4201 gggctcgccg agaccgaggc acgcgccgag tacgcagcgg agcaggtggt cggcgcggag
     4261 tcagggttga agtgcgcgtt atcgctcgcg cgccgcgtgg cgcgcaccga cgcgacggta
     4321 ctcgtgcgcg gcgagaccgg caccggcaaa gagatggttg cgcggctcat tcacgagcac
     4381 agcgcgcgca aggacggccc tttcgtgcgc gtgaattgcg cggcgctcgc ggaaggggtg
     4441 ctcgaaagcg agctcttcgg tcacgagaag ggcgctttca cgggcgcgaa ttcgctccgc
     4501 cgcgggcgct tcgagctagc gtgcggcggc acgatattcc tcgacgagat cggcgacgta
     4561 tcgcccaagc tccaatcgag tctgcttcgc gtgctgcaag agcgagaaat cgagcgggtg
     4621 ggcgcgagcg ctccggtgcc cgtaaacgtg cgcgtgatca ccgccacgca tcgagatctc
     4681 gaagggcgcg ttcgcgaagg cacgtttcgt gaagatttgt tttaccgcat caacgtagtc
     4741 cccatcgtcg tgccaccggt gcgagcgcgg ccagaagatg ttggaccgct ggttcggcac
     4801 tttctcgtga agcacgcgcc gccgggtcgt ccgcctgcct tcgtgtccga cgcaaccatc
     4861 gcgcggctat ccgaatatgc gtggcccggc aatgttcgcg agatcgagaa cttggtgcaa
     4921 cgagccgtga ttttggccgt tggcagcgag atcacgctcg aagacttttg cctgcgcctg
     4981 gatatcccgg aagacttgcc ggtgcgggag gcggt
//
LOCUS       DASTKS010000031         7789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_672922, whole genome shotgun sequence.
ACCESSION   DASTKS010000031 DASTKS010000000
VERSION     DASTKS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672922"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1376
                     /locus_tag="VFQ35_00630"
     CDS             231..1376
                     /locus_tag="VFQ35_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929152.1"
                     /translation="MSQPNPPSAPSGQPNLQIQGGAAIVAPVSAFPGGVPGNALIVLP
                     LNQPNDELAQAMKNGPVALEIDKMWQLLGFNGPAVQVQDADGRPIAVGLEDLLSGLEK
                     HWQEDADDLNRGRMYAQELMKYERFEAAEKVLAKIVAKGGSGEDWLALGISQVRQEKW
                     EKAEGTLRGAQSLLPDNPFPALHLAKALEGKGDSAGARSLIEQAISIAPNSVDAWAFL
                     FSEVQRKDGDEAAERAVSELANTEANAKTAAPFIAVQGIYGASEEKRDKALVFAKKAV
                     DRNDNDPLALICLSALYGQNGDLDNVIALLSKHEAKMASDVRLAHNYFEALFQKRQLD
                     RVTKLLNALAGSQTREVKQFAIERSRMVAQYLQQQQQQLAAASQRRA"
     gene            1647..2870
                     /locus_tag="VFQ35_00635"
     CDS             1647..2870
                     /locus_tag="VFQ35_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9929153.1"
                     /translation="MLTLALVVLWSLLVSFACSVAESVVLSISHAQIQALGKSSAGRI
                     LRQFKREVDVPIAAIVAFHTVAHTVGASVCGAMYVDVFNEHTLWVFSLTFTLLVLVFS
                     EIVPKTLGVTFIPQFAVPVAYGVSALTVVLKPVIWLTNSFARLLRGGHQAPATSIDEI
                     RLLVAFGRTEGALGGRLADMIEGAARLRELTAYDVMVPRSQIIFVSGERTLEENLSII
                     KRSGHSRFPYTPDGNLDKVEGVVLTKELFFGLREKGDIVDFRDIAGPLVVVPTSAPLE
                     KVLRTFQDERRHMAIVVDEYGGTQGIITLEDVLEEIVGEIEDESDRVDPSIVRRGPGF
                     YSCRGSAETRKVFELLGIEEESEMVTLAGFVADLVGRVPRVGDSVEWEGHRFTVTRAS
                     ARRAERVEIRKLPVA"
     gene            2983..4188
                     /gene="recA"
                     /locus_tag="VFQ35_00640"
     CDS             2983..4188
                     /gene="recA"
                     /locus_tag="VFQ35_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9929154.1"
                     /translation="MSVLDDKQKGKALELAIASVQKEFGEGAIMRLKDGEKIGGEVSV
                     IPSGSLGLDLALGIGGYPRGRIVEIYGPESSGKTTLTLHAIASVQRRGGVAAFIDAEH
                     ALDVTYAKKLGVRTDELLISQPDYGEQALEIADMLVRSGAVDLVVVDSVAALVPKAEI
                     EGDMGDSHVGLQARLMSQALRKVTGSVSRSQCMIFFTNQIRMKIGVMFGSPETTTGGN
                     ALKFYASVRLDVRRVGAIKEAGAEGKDPVVVGNRTRVKVVKNKLAAPFREVEFDILYG
                     QGVSRSGEVLDLAVDGSMVQKSGAWFSLDGERIGQGRDNARLYLEQHPELIDKLEQRV
                     LAHHGVRRPGDPPLPTEAKGAEAKGGEPKGADAARDGSKGKAEGESASKPVNGAAGAP
                     RRPPGPRPS"
     gene            4787..5644
                     /locus_tag="VFQ35_00645"
     CDS             4787..5644
                     /locus_tag="VFQ35_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929155.1"
                     /translation="MTPQLGFPWISAPKATQKLRIRPWLAPRMATAVLRVPDPRVVPL
                     QLPLLTPTGPQIFVHEGARQALERRFMAAFGGAVQLAVTDNRRRMVTHTESRGTLRVR
                     VHMMFLGAPDRVRDALVAYVVHSDRDASQIVGDYIDQNLHRIRASRPVPGPLKTRGEV
                     HDLVAILAELNAEYFGGALSDVLVTWTRRTKRSTKARKSIKLGTYSAVERLVRINPVL
                     DKPWVPRYFVAFIVYHELLHHVVPAQRVGRRSLLHSPEFCRREREFRHYERAMAWERK
                     HIDRLLRSS"
     gene            complement(5960..6757)
                     /locus_tag="VFQ35_00650"
     CDS             complement(5960..6757)
                     /locus_tag="VFQ35_00650"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HET9929156.1"
                     /translation="MIAPRLRNRKLVRTASFAVAAAASAVAVLAQTAIAKADTHDELG
                     HYERWRRSTESPQDVAVELRFGRYVPNADEGLNGTPYKSTFGDKNRYYVGAEVDWQLL
                     RIPMFGTLGPGIGAGYTIASANARFSDGSAVSKQTTSLAILPMYLVGVLRADVVARET
                     LVPVVPYAKLGLGYALWWSKDQEDTARVDGKVGRGASYGYQFALGAMFLLDVIDRADA
                     KAADAAIGLNHSYIFGEWFKSDLDGFGAKDRLQVGTSTWTVGLAMEF"
     gene            complement(6808..>7789)
                     /locus_tag="VFQ35_00655"
     CDS             complement(6808..>7789)
                     /locus_tag="VFQ35_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929157.1"
                     /translation="SVCVEARAQLTQTVVIQGTPTRREGREPIGIKPNVINYADCLAD
                     DKITFKLNALTGSSNITLEVWVGTSGADCGNVNRSPATIPSQCWQVYIATPWPTASFD
                     IPVREILPHNASGPGTSSPDVCDTVANDGTGNGNPVMYFVPVAGQAVSGGTGATYNIV
                     YDLKGPTPPTNFNVGLGDTRLIASWTTSGDRDITGYKLFCEATDLCASEVLVPDTVPP
                     TTLPDNVKSSTPGPNSTEGDVSGLENGQEYVCSIAGTDAYGNVGKLSGIACGTPKSVD
                     SYYKVYRAAGGEAGGGYCSFSRSANMAFPPFVLAAGLAFVARRKRRRSIA"
BASE COUNT         1388 a         2397 c         2586 g         1418 t
ORIGIN      
        1 gtggaggcgg ggatccgact gtccatggtc accgaatgcg acaaagcgcg cgggcctgtc
       61 aaagtctcgg cggggcaccg ggccgattcg aaagcctagc aattcgcgct attgccggga
      121 tctggccccg ctgctagtac cgcctgcgct cccgattgac tccggttttc cggacgcgcc
      181 gagcgggccg cttcttcgcc ggggcggcgt aaacacgagg taattcgttc atgagccagc
      241 ccaatcctcc gagcgccccc agcggtcagc cgaatcttca aatccagggc ggtgccgcaa
      301 tcgttgcccc cgtcagtgca tttccgggtg gggtgccggg caacgcgctc atcgtgttgc
      361 ccctcaatca gccgaacgac gaactcgctc aggccatgaa gaacggcccc gtcgcgctcg
      421 agatcgacaa gatgtggcag ctgctcggct tcaacggacc tgcggttcag gtccaagacg
      481 ctgacggtcg tccgatcgcc gtcgggctag aggatttgct ctctggtctc gagaagcact
      541 ggcaagagga cgcggacgat ctcaaccgcg gtcgcatgta cgcgcaggag ctcatgaagt
      601 acgagcgctt cgaggcggcg gagaaggtcc tcgcgaagat cgtggcgaag ggcggaagcg
      661 gcgaagactg gctcgcgctc ggcattagcc aggtccggca agagaagtgg gaaaaggcgg
      721 agggcaccct gcgtggcgct cagtcgttgc tgccggacaa tccgttcccg gcgctccacc
      781 tcgcgaaggc tctcgaaggc aagggcgaca gcgctggtgc tcgctcgctc atcgagcaag
      841 cgatttcaat cgctccgaac agcgtcgacg cttgggcgtt cctgttcagc gaggttcagc
      901 ggaaggacgg cgacgaagcc gctgagcgcg ccgtgagcga gctcgcgaac acggaagcca
      961 acgcgaagac ggcggcgccg ttcatcgccg tgcagggcat ctacggcgcg agcgaagaga
     1021 agcgcgacaa agcgctcgta ttcgcgaaga aggcggtcga ccgcaacgac aacgatccgc
     1081 tcgcgctcat ctgcttgtca gccttgtacg ggcagaacgg cgatctcgac aacgtgatcg
     1141 ctctgctctc gaagcatgaa gccaagatgg cgagcgacgt gcgtcttgcg cacaactact
     1201 tcgaggcgct gttccagaag cgtcagctcg atcgcgtcac taagctcttg aacgcgctcg
     1261 cgggttcgca aacccgcgaa gtgaagcagt ttgccatcga gcgctctcgc atggtcgccc
     1321 agtacctcca gcagcaacaa caacagctcg cggccgcctc gcagcggcgg gcctaacgct
     1381 gcctttgacg gcgcaagggg cggcgcgggc agacctcgtc gctccttcgt tcgctggctc
     1441 ggcccgggcc gaatccgtgc gtaccgcggg cgcagggccg gcttgcgctt ctatgtaggc
     1501 gagacccggg cgaaaatcgc ggtgtcgcct cggccttcgt cgtctgcctt cgggctaaag
     1561 ccgtcgctta cgagtccctc ggcgaatctt cgggtccgtc cactcgggca ccggtgtaag
     1621 ctcccgggcg cgccccgcgc tgattgatgc tgacactagc gctcgtcgtc ctctggtcgc
     1681 tcctggtttc ctttgcgtgc tcggttgccg agtcggtcgt gctcagcatc tcgcacgcgc
     1741 agatccaagc gctcggaaag tccagtgccg ggcgcatcct gcggcagttc aagcgcgagg
     1801 tcgacgtgcc catcgccgcg atcgtggcgt tccatacggt ggcgcacacc gtgggcgcgt
     1861 cggtgtgcgg cgcgatgtac gtcgacgttt tcaacgagca cacgctctgg gtgttctcgc
     1921 tgaccttcac gctactcgtg ttggtctttt cggagatcgt cccgaaaacc ctcggcgtca
     1981 cgttcattcc ccagtttgcg gtgcccgtcg cttacggcgt gagtgcgctc accgtcgtcc
     2041 tgaagccggt gatttggctc acgaactcat tcgccaggct acttcggggg ggccatcaag
     2101 ctcctgcgac ctcaatcgac gaaattcgtt tgctcgtcgc gttcggccgc accgaagggg
     2161 cgctcggcgg ccgcctcgcc gatatgatcg aaggcgctgc ccggttgcgc gagctcaccg
     2221 cctacgacgt gatggtgccg cgctcgcaaa tcatcttcgt ctccggagag cggacgctgg
     2281 aagagaacct ctcgatcatc aagcgctccg gtcacagccg ctttccctac acgcccgacg
     2341 gaaacctgga caaagtcgaa ggcgtggtcc tcactaaaga gctcttcttc gggctccgtg
     2401 aaaagggcga catcgtcgac ttccgagaca tcgcgggccc gctcgtcgtc gtgccgacct
     2461 cagcgcctct cgagaaggtg ctgcggacgt tccaagacga gcggcggcac atggccatcg
     2521 tcgtcgacga gtacgggggg actcagggca tcattacgct cgaagacgta ctcgaggaaa
     2581 tcgtcggcga gatcgaagac gagagcgacc gggtcgaccc cagcatcgtg cggcgtggcc
     2641 ccgggttcta ttcgtgtcgc ggctccgcgg agactcgcaa agtgttcgag ctccttggaa
     2701 tcgaggagga gagcgagatg gtgactctcg ctgggttcgt cgccgatttg gttggacggg
     2761 tgccccgagt gggcgacagc gtcgagtggg aaggccaccg tttcaccgtg actcgggcga
     2821 gcgcccgtcg cgccgagcgc gtcgaaatca ggaaattacc ggtggcgtga ggggcgtttt
     2881 cggcccacgg gatcggacgg attcggtccc gccgcttcgt tgacatctga acaaccgaac
     2941 agtataaccc ctctcgaatt caccgactgg aaggcaaagg tcatgagcgt gctggacgac
     3001 aagcagaagg gcaaagcgct ggagctcgcg attgcgagtg ttcaaaaaga gttcggcgaa
     3061 ggcgcgatca tgcgcctgaa agacggtgag aagatcggcg gagaggtctc ggtcatcccg
     3121 tctggttctc tcggcctcga tctcgcgctg ggcattggtg gctatccgcg cggtcgtatc
     3181 gtcgaaatct acggtcctga gtccagcggc aaaactacgc tcacgcttca cgccatcgcg
     3241 agcgtccagc gccgcggcgg cgtcgcggcc ttcatcgacg ccgagcacgc gctcgacgtc
     3301 acctatgcaa agaagctcgg cgtgcgcacg gacgagctcc tcatcagcca acccgactac
     3361 ggcgagcaag cgctcgaaat tgccgacatg ttggtgcgct ccggcgctgt cgacctagtc
     3421 gtggtcgatt cagttgccgc gctcgtaccc aaggctgaaa tcgaaggtga catgggcgac
     3481 tcccacgtcg gtctacaagc gcgcctcatg agtcaggcct tgcgcaaggt gaccgggtcg
     3541 gtctcgcgct cgcagtgcat gatcttcttc accaaccaga tccgcatgaa gatcggcgtc
     3601 atgttcggca gcccggagac gactacgggc ggcaacgcgc tcaagttcta cgcctccgtg
     3661 cgcctcgacg tgcgccgcgt gggcgccatc aaagaggccg gagccgaggg gaaggatccg
     3721 gtggtggtcg gtaatcgcac gcgcgtgaaa gtggtcaaaa ataagctcgc ggcgccgttc
     3781 cgcgaggtcg agttcgacat cctttacggc cagggcgtga gccgttcggg cgaagtactc
     3841 gacctggcag tcgacggcag catggtgcaa aagagcggcg cctggttctc gttggacggt
     3901 gagcgcatcg gccagggtcg cgacaacgcg cggctttatc tcgagcaaca tccggagctc
     3961 atcgacaagc tcgagcagcg agtgctcgct caccacggtg tgcgtcgccc gggcgatccg
     4021 ccgctgccca ccgaggctaa gggcgccgaa gcaaagggcg gcgagcccaa gggtgccgac
     4081 gctgctcgcg atggaagcaa aggtaaggcc gaaggcgaga gcgcgtccaa gcctgtcaac
     4141 ggggcagcgg gcgcgccgcg aaggccacct gggccccgtc cgagctgaag ctcgccatgg
     4201 gtgaggtgcc ggaaggcgcc gtttcgaacg accgagagcc cacccgcggt cgcggcatct
     4261 cgccggttgc tcgcgtcaat gaacgccgct cgggaacact gacgcgagca acttcagcct
     4321 ggggcgcgca cgcgctctag ggcattgtcg ctcgactttc agtctggcga atcgattggg
     4381 atgtcgcgcg ccaggctgtc ttgatttcca aaaggggtac tgcgctgttg caccagcgcg
     4441 aggccgattg gcaattcggt gtgtctcgct ttcgccaatc ggttcgatgg gggcgtcggg
     4501 gagtgaggat cgtggcccgt ctcgggtgaa aggaggcgtg taacctgtgg ataaggtgcg
     4561 aggatttctg ggtttttgga cactgcaggg ggcgaacccc cagggagggt ttcccgcgat
     4621 gaatccgagc tcggcggcac ggaaccccat tcagaacggg tacttaggct gggcgaaatg
     4681 accggggggt caaatcgtta gaagtgtccg gaattaaacg caaaaatgca attccttgac
     4741 gctaagcaac ccaaccccta gtgcgaatgg agcaccccgt ttccccatga cccctcagct
     4801 cggtttcccc tggatctccg caccgaaagc gacgcagaag cttcgcattc ggccgtggct
     4861 cgcaccgcgc atggcaacgg ccgtgttgag ggtgccagat ccgcgtgtgg tgccgctgca
     4921 actaccgttg cttactccaa cggggccgca gatcttcgtt catgaggggg cgcggcaggc
     4981 gctcgagcgg cgcttcatgg cggcgttcgg cggggcggtg cagctcgccg ttaccgacaa
     5041 ccgacgtcgc atggtcaccc acaccgagag ccgcggcacg ctccgggtgc gggtgcacat
     5101 gatgttcttg ggtgccccgg atcgagtgcg cgacgccctg gtcgcgtacg tggtgcacag
     5161 cgatcgagac gcctcgcaga tcgtcggcga ctacatcgac cagaacctgc accgcatccg
     5221 agcgtcgcga ccggtgccgg ggccgctgaa gacccgcggg gaggtgcacg acctcgtagc
     5281 catcttggca gagctcaacg ccgagtactt cggtggcgcg ctatcggacg tgctggtcac
     5341 gtggacacgg cgcaccaagc gctccaccaa ggcacgcaag agcatcaagc tcgggaccta
     5401 cagcgccgtc gagcggctgg ttcgcatcaa tccggtcctc gacaagccgt gggtacctcg
     5461 ctacttcgtc gcgttcatcg tctatcacga gctcttgcac cacgtcgttc ctgcacagcg
     5521 cgtcggccgc cgctcgctct tgcactcacc ggagttctgc cgacgcgagc gcgaattcag
     5581 gcactacgag cgcgcgatgg cttgggagcg caaacacatc gaccggctcc ttcgctctag
     5641 ctgagctgtc ccagggcagg tgctcgcggg ttcgcacttg tcccagggca ggtgctcgcg
     5701 gtttggcact tgtccagggc aagtgctgga gggttcggca cttgtcccag ggcaagtgct
     5761 cacggtggtt actcgtctca gggcaagtac ctgtcccagg gcaggtgctc tctggcccgc
     5821 acttgtccca gggcaagtgc tcgcggagaa cggagcggtg gttacccgcc tcagcagagc
     5881 aagtacctgt cccagggcag gtgctgggct cggggacgcc gcagggttcg gttttcgagc
     5941 gagcggggag gctcttcgct cagaattcca ttgctagacc gacggtccag gtgctggtgc
     6001 cgacttgcag gcgatccttg gcgccgaagc catcgaggtc gctcttgaac cattcgccaa
     6061 agatgtagct gtggttgagg ccgatggcgg cgtcggcggc tttggcgtcg gcgcgatcga
     6121 tgacgtcgag caagaacatc gctccgagcg cgaattgata gccgtacgaa gcgccccgcc
     6181 cgaccttgcc gtccacgcga gcggtgtctt cttggtcctt ggaccaccaa agagcgtagc
     6241 cgagcccgag cttcgcatag gggacgacgg gtacgagcgt ttcgcgcgca acgacgtccg
     6301 cgcggaggac gccaaccaaa tacatcggaa ggattgctag ggatgtcgtc tgcttagaga
     6361 cggccgagcc gtccgaaaag cgcgcgttgg cactagcgat cgtgtagcca gcgccgatgc
     6421 cgggaccgag ggtgccgaac atggggatgc gcaagagctg ccagtcgacc tccgcaccga
     6481 catagtagcg gttcttgtcg ccgaaagtgc tcttgtaggg cgttccgttc aggccttcgt
     6541 cggcgttggg cacgtagcgg ccgaagcgga gctctaccgc gacgtcctgc ggtgactcgg
     6601 tcgagcgccg ccagcgttcg tagtgaccga gttcgtcgtg agtgtcggct ttggcaatcg
     6661 ccgtctgcgc gagcacggcg acagcgcttg cggccgcggc gacggcgaac gaggcggtgc
     6721 ggacgagctt ccggtttcgg aggcggggcg cgatcatcga aggggctcgg tccaaaggtt
     6781 gatgggggag ttcgtgctcg ctcggcatca tgcgatgctc cggcgacgtt tgcgacgagc
     6841 aacgaaagcg agccccgcag cgagaacgaa aggcgggaat gccatgtttg cgctgcgcga
     6901 gaaagagcag tagccgccgc ccgcctcccc acccgccgcg cggtaaacct tgtaataaga
     6961 gtcgaccgac ttcggcgtac cgcaggcgat gccggaaagc ttgccgacgt tgccgtaagc
     7021 gtcggtgccc gcgatcgagc atacgtattc ttggccgttc tcgagcccgc tcacgtcgcc
     7081 ctccgtcgag ttgggcccag gcgtcgagct cttgacgtta tccggcaggg tggtgggggg
     7141 gaccgtatct ggtacgagga cttccgacgc acagaggtcg gtcgcctcgc aaaagagctt
     7201 gtagccggtg atgtcacgat caccgctggt ggtccacgac gcgatgaggc gcgtgtcgcc
     7261 cagacccacg ttgaagttgg tcggtggcgt tggccctttc agatcgtaga cgatgttgta
     7321 cgttgcgccc gttccgccgg atacggcctg acctgcgaca gggacgaagt acatgacggg
     7381 gttgccgttg ccggtcccgt cgttcgcgac ggtgtcgcag acgtcggggc tactcgtgcc
     7441 gggaccgctc gcgttgtgcg gcaagatctc gcgcactgga atgtcgaagc tcgcggtcgg
     7501 ccacggcgtt gcgatgtaaa cctgccagca ctgactaggg atggtcgcgg gagagcgatt
     7561 gacgttgccg cagtctgccc ccgacgtacc cacccagact tccagagtga tgttgctgct
     7621 gccggtgagc gcgttgagct tgaaggtaat cttgtcgtct gcgaggcagt cggcgtagtt
     7681 gatgacgttc ggcttgatgc cgatcggctc tctcccctcg cgccgcgttg gcgtgccttg
     7741 gatgactacg gtctgagtga gctgagcgcg tgcctcgacg cacacggaa
//
LOCUS       DASTKS010000032         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_672945, whole genome shotgun sequence.
ACCESSION   DASTKS010000032 DASTKS010000000
VERSION     DASTKS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672945"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFQ35_00660"
     CDS             <1..1010
                     /locus_tag="VFQ35_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929158.1"
                     /translation="VAALALGLAVASAIASACGNGDAGPSRTANGAGGSPVIIISGSG
                     GTNVNVENPTTTVDGGRPMRCDGNGQCACINVASFGKPAHYGNANDNTDAFQTWLNTK
                     SSARVDLVRTHTPLTAELLRNYDVLVLQALEDNEGGPFWTFSADEIKAVSDWVNAGGG
                     IISLMGYGGDAREVEPTNALLAFSGISYNKDDILAQCPNNLCYCWGNTIPISGFLGDH
                     PIAKNLTQIGGFHGRSINAGGASVIAKSGDAVVAAGKTIGQGKVFVFADEWITYTSQW
                     LGSGQQQTVNVNDPCYDPAHDYMMTADKVFQVPQFWYNAIKWAAPPTQCFTIDNPTIV
                     K"
     gene            complement(1017..1547)
                     /locus_tag="VFQ35_00665"
     CDS             complement(1017..1547)
                     /locus_tag="VFQ35_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9929159.1"
                     /translation="METKITLRPYEPSDANDVFVAVQESMTELSRWMPWCHPGYSYAD
                     ALSWIRLTQEGRATGKMYDFGVFDATGRYVGGCGITSVNAVERFANLGYWVRTSRTGG
                     GLASAAARDVARWAFANTPLNRLEIVVAVGNVRSERVALKAGAEREGVLRQRLVLDGR
                     SRDAHMFSIVRSSSAL"
     gene            1913..3025
                     /locus_tag="VFQ35_00670"
     CDS             1913..3025
                     /locus_tag="VFQ35_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9929160.1"
                     /translation="MSTDVMIEASALTKRFGVARALDKVSFEVRRGEVVGFLGPNGAG
                     KSTTMRILTCFISPTSGSAKVHGHDVFDSPLAVRRAIGYLPQRAPLYTDMSVYDYLRF
                     VAEIRGLSDSTFKTRLKKVVEVCGLAQSLGKDIGTLSHGYRQRVGLGQALIHDPPILI
                     LDEPTSDLDPNEKAEVLKYIKEIGKDRTILLSTHNLGEVEEACARAIIVSKGRVVADG
                     ALDDIRGKTGRVRYVIAVDEQRAEGSHQPPSADAARSALEKISGARSVRELPTDEKAH
                     KFELVGAQDSDLRPEIFRLVVAKGWTLLELRRDAQSLDAVFRDLTRGDEKLDRGADWK
                     SEQGDEPEEEETDSDDDDEDDEDDADDDNHAPARKA"
     gene            3037..3777
                     /locus_tag="VFQ35_00675"
     CDS             3037..3777
                     /locus_tag="VFQ35_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929161.1"
                     /translation="MLTIAKREFRSYFDSPLAYVVICLSFFALGLMFFMLNGGFWQAD
                     RSSVERLFEYAPMGLSFLVIPVVTMRLVAEERRAGTLEMLITLPVSDSDVVLGKFFGA
                     LGLVLVLVLATLVYPIGMFKWPWVLGPLDSGPVVSGYFGLILFSAAATSIGLLVSALS
                     ESQAVAFFITFFVLAALWFFDNAAQYLGSASGGGTLPNLLQYMSFQTRLSGFWRGLVD
                     SRDVVFFLSVTILSLLVAFRALERRKWA"
     gene            3780..5633
                     /locus_tag="VFQ35_00680"
     CDS             3780..5633
                     /locus_tag="VFQ35_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET9929162.1"
                     /translation="MASQEKKRKDAAQAGLYLAVIVAIVVVANMLSSKLYVRSDRTQA
                     ERYTLSQGSGRLLQGLKGPVEVDAYIKTGLPQLDAFVRDLTDLLKEYERAGKGKFKFT
                     LIEPNTDELRQQAKDAGLQEQPFGEVSATGEDQASIAQGYLGLVFKYGSEKAVIPQLF
                     PNQTDGLEFWITNKIREIRDKADNIKHRIGVITGKDELKLTDTNLVPKQGRGGGSPSL
                     QAILEQAFPFYKIEEVDLKDGASEIDKELVGLIITQPQKDYSEKELRRIDEFLMRGGK
                     SLAVYASAVTLKANDASMKATLSTHGLDKLLAGYGITINKDAVLDYGAQFRIGAISPQ
                     TGAVTWVRHPGIAHVVNDPRLSEDEKALDTSFAGFFRMEEAIFPFPSSLTLDKKKQPA
                     DVKLYPVARTTPQAGVDTSESIDMKMRDRWLPKGKQEQRVIAAVAEGKLKSAFADAPS
                     EEVKPNATAPTPSRVLVVSSSEFLTNPFAYAGNGPELGAQFQMFGAVGGDPQLQQIGQ
                     FYTKHLTSTILSFKNTLDWISGDADLLAASAKLIGVPSLSYQSVAKPKFKEGDDEAEA
                     KRKDEEYKAMRKSMQFRVQWALTLGAPLLFALFGLFRWRQREAERENLKAA"
     gene            5654..>6040
                     /locus_tag="VFQ35_00685"
     CDS             5654..>6040
                     /locus_tag="VFQ35_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET9929163.1"
                     /translation="MATTETKLVVALGVLAVLGGGLYMTNKKQAEEAKAYSLNARAAD
                     LPKLELSDDDVNKISKVVLHRPAKEGAAAVDVTLVKKGEEWRVSAPVDAEANQSNVKS
                     MLDNLKTIKVSEVIEPGKADYAKYKVS"
BASE COUNT         1195 a         1945 c         1898 g         1002 t
ORIGIN      
        1 aagtggccgc attggcgctg gggcttgccg tcgcttctgc catcgcgtca gcctgcggca
       61 acggcgatgc ggggccctcg cgaacggcga acggcgccgg cggttcgccc gtcatcatca
      121 tatcgggttc gggcggaacg aacgtgaacg tcgaaaaccc gacgacgacc gtcgacggcg
      181 gccgccccat gcgctgcgat ggcaacgggc agtgtgcctg catcaacgtt gcgtcgttcg
      241 gcaagcccgc gcattacggc aacgcaaacg acaacaccga cgcgtttcag acctggctca
      301 acacgaagag cagcgcgcgc gtggacctgg ttcgcacgca cacgccgctc accgccgagc
      361 tcttgagaaa ctacgacgtg ctcgtgttgc aggcgctcga agacaacgag ggtggcccgt
      421 tctggacctt cagcgccgac gagatcaagg ccgtgtcgga ttgggtcaac gccggcggcg
      481 gcatcatctc gctcatgggg tacggaggtg acgcgcgcga ggtcgagcca accaatgcgc
      541 tcctcgcgtt ttccgggatc tcttacaaca aggacgacat cctcgcgcag tgcccgaaca
      601 acctttgtta ctgctggggc aacaccatcc cgatctcggg cttcttgggc gaccacccca
      661 ttgccaagaa cctcacccaa atcggcggct tccacgggcg atccatcaat gccggcgggg
      721 cgagcgtgat cgcaaagagc ggcgacgcgg tcgtggccgc ggggaaaacg atcggacagg
      781 gcaaagtctt cgtttttgcc gacgaatgga tcacgtatac gagccagtgg ctcggaagcg
      841 ggcagcagca gaccgtcaac gtcaacgacc cctgctacga ccccgcccac gattacatga
      901 tgacggcgga caaggtcttc caagtgccgc agttttggta caacgccatc aagtgggcgg
      961 caccgccgac gcagtgtttc acgatcgaca acccgactat cgtgaagtga gatagctcaa
     1021 agtgctgatg aagagcgcac gatcgagaac atgtgcgcgt cgcgcgaccg accgtcgagg
     1081 accaagcgct gacgcagcac gccttcgcgc tcggcgcccg ctttgagggc gactcgttcg
     1141 ctgcgcacgt tgccgaccgc gacgacgatc tcgagccgat tcagtggcgt gttcgcgaac
     1201 gcccagcgcg cgacgtcacg cgctgctgcg gacgctagtc ctccgccggt gcgcgaagtg
     1261 cgaacccagt agccaaggtt cgcgaagcgc tccaccgcgt tgacggaggt gatcccgcag
     1321 ccacccacgt agcgaccggt ggcgtcgaat acgccgaagt catacatctt gccggtcgcg
     1381 cggccttctt gcgtgagccg gatccaactc agcgcgtcgg cgtaggagta gccggggtga
     1441 caccacggca tccagcgcga cagctcggtc atcgactcct gcaccgcgac gaagacgtcg
     1501 ttcgcgtcgc tcggctcata aggacgaaga gttattttcg tctccatctg agactcgaat
     1561 ctagccgggg gcggccggcg ggactctgcc aaaccctccc agccgccgcg tcgcgcagag
     1621 agctgcgaat tcgcctcggg gcttggacat tggcttcgtg gccgagggag ggcgaattcg
     1681 cctgccgcgg acagaaagag tgaagagtcc gaaagggctc gggaatagcg cggcgcgccg
     1741 caggttgatg gcgtacccgc cgagacacac gcgagatgga gctgtggacc gttgacagag
     1801 atcgagccgc cgctaggatg ccgcggccca gcgcccccag agtgggtcgg acgtgggtgt
     1861 tctggttctc tcccgcctcg taacgggggc gttttttacg atgggaggtc ggatgtctac
     1921 cgacgtcatg atcgaagcga gtgcactgac caagcggttc ggtgtggctc gcgcactcga
     1981 caaagtgagc ttcgaagtcc gccgcggcga agtggttggg ttcttgggac cgaacggtgc
     2041 cggcaaatcc accacgatgc gcatcctcac gtgcttcatc tcgccgacca gcggctcggc
     2101 gaaggtacac gggcacgatg tgttcgattc gccgctcgcg gtgcgtcgcg ccatcggcta
     2161 cctgccccag cgcgcccctc tctatacgga catgtccgta tacgactacc tgcgcttcgt
     2221 cgcggagatc cgaggcctct cggacagcac cttcaagacg cgcttgaaga aggtggtcga
     2281 ggtgtgcggc ctcgcccagt ctctcggcaa ggacatcggt accctttcgc acggctatcg
     2341 gcagcgcgtc ggcctcgggc aagcgctcat tcacgacccg cccatcttga ttctcgacga
     2401 gcccacttcg gatctcgacc cgaacgagaa ggccgaggtg ctgaagtaca tcaaggaaat
     2461 cggcaaagat cgcacgattt tgctctcgac ccacaacctc ggtgaggtcg aagaagcgtg
     2521 cgcgcgcgcg atcatcgtca gcaagggccg cgtggtcgcg gacggcgcgc tcgacgacat
     2581 ccgcggcaag acggggcgcg ttcgctacgt catcgccgtc gacgagcagc gcgccgaagg
     2641 ctctcatcag ccgccatccg ccgacgcagc ccgctcggcg ctcgagaaga tctccggcgc
     2701 gcgctccgtt cgcgagctgc cgaccgacga gaaagcccac aagttcgagc tcgtgggcgc
     2761 gcaagactcg gatcttcgcc cggagatctt ccggctggtg gtcgcgaagg gatggacgct
     2821 gctcgagctc cgtcgcgacg cccagagcct cgacgcggtg ttccgcgacc tgacgcgcgg
     2881 cgacgagaag ctcgatcgcg gcgccgattg gaagagcgaa caaggcgacg agccagaaga
     2941 agaagagacc gattcggacg acgacgacga ggatgacgaa gacgacgcgg acgacgataa
     3001 ccacgccccc gcgcgaaagg cttagccatg tctccgatgc taactatcgc aaaacgcgaa
     3061 tttcgttcgt atttcgactc gcccctggct tacgtggtca tctgcctgag cttcttcgcg
     3121 ctcgggctca tgttcttcat gttgaacggt ggcttctggc aggcggaccg ctcttcggtg
     3181 gagcgcctgt tcgagtacgc cccgatgggg ctgagcttcc tggtgatccc ggtggtcacc
     3241 atgcgcctcg tcgcagaaga gcgccgcgcc ggaacgctcg agatgctcat cacgctgccg
     3301 gtcagcgaca gtgacgtggt gctcggcaag ttcttcggcg cgctcggcct cgtgctggtg
     3361 ctcgtcctcg ccacgctcgt ctaccccatc ggcatgttca agtggccgtg ggtgctcgga
     3421 ccgctcgatt cgggtccggt cgtgagcggc tacttcgggt tgatcttgtt cagcgctgcc
     3481 gccacttcga ttgggctctt ggtcagcgcg ctgagtgaga gccaggccgt cgcgttcttc
     3541 atcacgttct tcgtgctcgc ggcgctctgg ttcttcgaca acgcggctca gtacctgggc
     3601 agcgcgagcg gcggcgggac gctgccgaac ctgctgcagt acatgagctt ccagactcgc
     3661 ttgagcgggt tctggcgcgg tttggtggac agccgcgacg tcgtgttctt cctgtccgtc
     3721 accatcctct ctctgctcgt cgctttccgc gcgctcgagc gccgcaagtg ggcgtgaaca
     3781 tggctagcca agaaaagaag cgaaaagacg ccgcccaggc agggctctac ctggcggtga
     3841 tcgtggcgat cgtagtggtc gccaacatgc tctcctccaa gctctacgtg cgttcggatc
     3901 gcacgcaggc cgagcgctac accttgagcc agggcagcgg tcgcttgctg cagggcctca
     3961 agggtccggt cgaggtcgac gcctacatca agacggggct cccccagctc gatgcgttcg
     4021 tgcgtgatct caccgacctc ttgaaggagt acgagcgcgc cggcaagggc aagttcaagt
     4081 tcaccttgat cgagccgaac accgacgagc tgcgccaaca agcgaaggac gcgggcttgc
     4141 aagagcagcc cttcggcgag gtcagcgcga cgggcgaaga ccaggcgtcg atcgctcagg
     4201 gctacctcgg cttggtattc aagtacggaa gcgaaaaggc ggtcattccg cagctcttcc
     4261 cgaaccaaac cgacggcctc gagttctgga tcacgaacaa gatccgtgaa attcgcgaca
     4321 aggccgacaa catcaagcac cgcatcggcg tcatcaccgg taaggacgag ctgaagctca
     4381 ccgacacgaa cctcgtgcca aagcagggac gcggcggtgg ctcaccgagc ttgcaagcga
     4441 tcctcgagca ggctttcccc ttctacaaga tcgaggaagt cgacttgaag gatggggcga
     4501 gcgagatcga caaggagctc gtcggcctca tcatcaccca gccgcagaag gactactccg
     4561 aaaaggagct gcgtcgcatc gacgagttct tgatgcgcgg cggcaagtcg ctcgccgtct
     4621 acgcttcggc ggtaacgctg aaagcgaacg acgccagcat gaaggcgacg ctgagcaccc
     4681 acgggctgga caagctgctc gccggctacg gcatcaccat caacaaggac gccgtgctcg
     4741 actacggcgc ccagttccgc atcggcgcga tcagcccgca aactggcgcg gtcacctggg
     4801 tacgccaccc cggcatcgcc cacgtcgtga acgacccacg cctctccgaa gacgagaagg
     4861 cgctcgacac gagcttcgcc ggcttcttcc gcatggagga ggcgatcttc ccgttcccgt
     4921 cgagcctcac gctcgacaag aagaagcagc ccgccgacgt gaagctctac ccggttgcgc
     4981 gcacgactcc gcaagccggt gtcgacacga gcgaaagcat cgacatgaag atgcgtgatc
     5041 gctggctgcc gaagggcaag caagagcagc gcgtcatcgc cgccgtcgcc gaaggtaagc
     5101 tgaagagcgc cttcgccgat gcgccctcgg aagaggtgaa gccgaacgcc accgcgccga
     5161 ctccgtcgcg cgtgctggtg gtctcgtcga gcgagttcct cacgaacccg ttcgcctacg
     5221 ccggaaacgg gcccgagctc ggcgctcagt tccaaatgtt cggcgcggtg ggcggcgacc
     5281 cccagctcca gcagattggc cagttttaca cgaagcacct caccagcacg atcctctcgt
     5341 tcaagaacac actcgactgg atatcgggtg atgcggacct gctcgccgcg agcgccaagc
     5401 tcatcggcgt cccttcgctc agctaccaga gcgtcgcgaa gccgaagttc aaggaaggcg
     5461 acgacgaagc cgaggccaag cgcaaggacg aagagtacaa ggcgatgcgc aagagcatgc
     5521 agttccgcgt gcagtgggcg ctcaccctcg gcgcgccgct cttgttcgct ctcttcggcc
     5581 tcttccgctg gcgtcagcgt gaagccgaac gagaaaacct gaaggctgcc tgaggaacgg
     5641 ggaacaatcg aagatggcaa ccacggagac caaactcgtc gtcgcgctcg gcgtcttggc
     5701 agtgctcggc ggcgggctct acatgaccaa caagaagcag gcggaggagg cgaaagccta
     5761 ctcactcaac gcccgcgccg ccgatctacc caagctcgag ctcagcgacg acgacgtgaa
     5821 caagatcagc aaagtcgtcc tgcatcgccc cgccaaagaa ggcgccgcag ccgtcgacgt
     5881 gacgctggtg aagaagggcg aagagtggcg cgtctcggcg ccggtcgacg ccgaggccaa
     5941 ccagtcgaac gtcaagtcga tgctcgacaa cctcaagacc atcaaggtca gcgaggtcat
     6001 cgaacccggc aaagccgact acgccaagta caaagtctcg
//
LOCUS       DASTKS010000033         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1119308, whole genome shotgun sequence.
ACCESSION   DASTKS010000033 DASTKS010000000
VERSION     DASTKS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119308"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFQ35_00690"
     CDS             <1..642
                     /locus_tag="VFQ35_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929164.1"
                     /translation="TVSGQVLEQMDVDKYSYLRVGEPGSQGTWTAVPVTSQQVGKRVR
                     VTSAQMMSNFTSTTLKRTFDVIYFGVLEGNSGQRIAESQTAPPHEANEAPHVGAGSAA
                     DKVPVAKVKRAEGPLGHTVAELVEGKNGGQKVRVRGIVVKQTPGVFGKTFLHLRDGSG
                     SAENGTNDLAVTTDAEPAVGSEVLLEGTLTLDKDFGSGYRYHVLLADAVAVQP"
     gene            complement(658..1623)
                     /locus_tag="VFQ35_00695"
     CDS             complement(658..1623)
                     /locus_tag="VFQ35_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9929165.1"
                     /translation="MKVLKVIAVGVLALVLVALGGIFYFKAAAKQRLAKDYRVAAASI
                     PIPYPLSPDEIEKLAPEQRAPAALQALAQERAVARGKHYVESRAACAECHGTDFGGKV
                     IVDNPAMGRWIAPNITRGGVTKSYRAEDWDRIMRHGILPSGKPAVMPAADFAAFSDQE
                     VSDIASYIHSLPPVARVMPKSELGPIYSFLIVSGKLPLSAETIEHGTSHAALPPTLAV
                     SNELGKHLALTCMGCHGEGLSGGKIDAGDPAWPPARNITFDDTGLGKWSLEDFRKALK
                     EGVRPDGTKLDPAMPVAYTARLQLEEVDALYLYLKSVPKKSYGNH"
     gene            1821..2654
                     /locus_tag="VFQ35_00700"
     CDS             1821..2654
                     /locus_tag="VFQ35_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929166.1"
                     /translation="MTAAVLSSSTQPRPLVPRWARGLPLLLFALAGCRHKEKRVLRTE
                     PWAAPVAISSASGAALPSAPEGKPVRYIVDQARVTLELPAKKKKPSGSLAKVSGEIDL
                     DVLHPENTRAELRADLLSLSLGSNGRDESPELVAKAFDWLELSARKPPEEREKERYAK
                     IEILGFDPVGGPDAAPQGRSARVVARGNLTLHHFRVPIALELDVQLRGGTTDEPSRLT
                     IRTRRPLVVSLVAHDILPRDAAGSLVAGGVRSLGTEVGRDARVTAEISAVASGAPGQK
                     Q"
     gene            2730..3122
                     /locus_tag="VFQ35_00705"
     CDS             2730..3122
                     /locus_tag="VFQ35_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929167.1"
                     /translation="MTHPLSFHQFRNTLAALGATGLVACAGGNEPAQSPVEAKEVPAA
                     APAAASAAPTNDASSAEAAATAPAAEKPAADPAAAPAPAAAAEAKPAEAAPAAAAPAK
                     PAQDTKKAAAAKHKAAGAKGGCGAGTCS"
BASE COUNT          570 a         1079 c         1091 g          526 t
ORIGIN      
        1 accgtgagcg ggcaagtcct cgagcaaatg gacgtggata agtactcgta cttgcgcgtc
       61 ggcgagcccg gctcgcaagg cacctggacc gcagtgcccg tcacctcgca gcaggtcggc
      121 aagcgcgtgc gcgtcacgtc ggcgcaaatg atgagcaact tcacgagcac gacgctcaag
      181 cgaacgttcg acgtcatcta cttcggtgtg ctcgagggga actcaggaca gcgcatcgca
      241 gagtcgcaga ccgcgccgcc gcacgaggcc aacgaggcac ctcatgtcgg cgccgggtca
      301 gccgcggaca aggttcccgt cgccaaggtg aagcgcgcgg agggcccgct cggacacacc
      361 gtcgccgagc tcgtagaagg gaaaaacggt gggcagaagg tccgcgtgcg cggcatcgtc
      421 gtcaaacaga cgccgggcgt gttcggaaag accttccttc acctgcgcga cggcagcggc
      481 agcgccgaaa atggaacgaa cgacctagcc gtaaccaccg acgcggagcc ggcggtcggc
      541 agcgaggtcc tcctcgaagg aactctcacg ctcgataagg acttcggatc cggctaccgc
      601 tatcacgtgc tcttggccga cgcggtcgcg gtccagccgt aaggcgcgcc ttgggcttca
      661 gtgattgccg taactctttt tggggacgct cttcaggtag aggtagaggg cgtcgacctc
      721 ctctagctga agccgcgccg tgtacgcgac gggcatcgcg gggtcgagct tggtgccgtc
      781 cgggcgcacg ccctctttga gagccttgcg gaagtcctcg aggctccact taccgaggcc
      841 agtgtcgtcg aaggtgatgt tgcgcgccgg cggccaagct ggatccccgg cgtcgatctt
      901 cccacccgac aagccttcgc cgtggcagcc catgcaggtg agggcaaggt gctttccgag
      961 ctcgttgctc accgcgagcg tgggcggcag cgccgcgtgt gaggtgccgt gctctatcgt
     1021 ttccgcgctg agcggcagct tcccgctcac gatgagaaac gaatagattg ggccgagctc
     1081 cgacttgggc atcacgcgcg cgaccggcgg caggctgtgg atgtacgagg cgatgtcgct
     1141 cacctcttgg tccgagaacg cggcaaaatc cgcggctggc atcacggctg gcttccccga
     1201 cggcaaaatg ccgtgccgca tgatgcggtc ccaatcctcg gcgcggtagc tcttggtcac
     1261 gccgccgcgg gtgatgttgg gagcgatcca ccgacccatc gccgggttgt cgacgatgac
     1321 tttgcccccg aaatcggtgc cgtggcattc ggcgcacgca gcgcggctct cgacgtagtg
     1381 tttgccgcgc gcgaccgcgc gctcttgcgc gagcgcctgg agggcggccg gggcgcgctg
     1441 ctctggcgca agcttctcga tttcgtcggg cgagaggggg tacggtatcg ggatgctcgc
     1501 cgccgcgacg cgatagtcct tggcgagtcg ctgcttggct gccgccttga agtagaagat
     1561 cccgcccagg gctacgagga cgagagcaag tacaccgact gctatgacct tcaagacctt
     1621 cattcgcggc tccaagagat gtccaaggcg accgacggtt cgtcgcgtcg gccagggcgc
     1681 gctttatagc gaatcccctt caacgcttca tcctcagaag ttcttaagag ccgaaccttg
     1741 agaacatgat cagctcgccg atttacgaac agccggggag cgcggatcgc acgccgtcgc
     1801 cggtttcatc gagatgtggc atgacggcgg cagtgctgag ctcttcgacc cagccgcgtc
     1861 cgctcgtccc gcgctgggct cgcggtttgc cgcttctgct attcgcgctc gccggctgcc
     1921 gtcacaagga gaagcgcgtc ctccgtaccg agccctgggc ggcgcccgtc gcgatctcgt
     1981 ctgcttccgg agcggcgctg ccgagcgcgc cggagggcaa gccggtgcgc tacatcgtcg
     2041 accaggcgcg ggtcacgctg gagttgcccg ccaagaaaaa gaagccgagc ggctccctcg
     2101 cgaaggtatc gggagagatc gatctcgacg ttctgcaccc cgaaaacacg cgtgccgagc
     2161 tgcgtgcgga tctgctctcg ctttcgctcg gcagcaacgg gcgcgacgaa tcccccgagc
     2221 tcgtcgcgaa agctttcgat tggctcgagc tctctgcgcg caaacctcct gaggaacgcg
     2281 aaaaggagcg ttacgcgaag atcgaaatcc tggggttcga tccggtggga gggccagacg
     2341 ccgctcccca aggccgctcg gcacgggtcg ttgcgcgcgg aaacctgacg ttgcaccact
     2401 tccgagttcc gatcgcattg gaactcgatg tgcaactgcg gggcggaacc accgacgaac
     2461 ctagccggct cacgatccgg acgcgccgcc cgctcgtagt gtccctcgtg gcgcacgaca
     2521 ttttgccgcg ggatgccgcg ggttctctcg tcgcgggcgg cgtccggagc ttgggcaccg
     2581 aagtcggacg agacgcgcgg gtaacggctg agatttcggc ggttgcgagc ggcgccccgg
     2641 gccaaaaaca atgaaactcg aggacgcgct cggcgtaacc tggcgcacgc ggctacatcg
     2701 ccgcggagga tataaggagt cgcactgaga tgacgcaccc gctctcgttc catcagttcc
     2761 gaaacacgct cgctgcgctc ggagccaccg gtttggtggc atgcgccggt ggcaacgagc
     2821 ccgcgcaaag cccggtcgag gcgaaagagg ttcccgcggc tgcgcccgcc gcggccagcg
     2881 cagcgcccac gaacgacgca agctcggcag aagctgccgc gaccgcgccc gccgccgaaa
     2941 agccggcggc cgaccctgcc gcagcgcccg cgcccgccgc ggctgcagag gctaagcctg
     3001 ccgaagcagc ccccgcggcg gccgcacccg cgaagcccgc tcaggacacg aagaaggccg
     3061 ctgcggccaa gcataaagcc gccggcgcca agggcggctg cggtgccggg acctgcagct
     3121 gagctcccca tgattgacgt ggcccgcggt cgaaacggca tcggcctcgg gctgcgctgg
     3181 gaattcctcg aggaagtcgt cgatgggcct ttgctcgacg tcgccttctt cgaggtctcg
     3241 cccgaaaact acatgcgccg cggcgg
//
LOCUS       DASTKS010000034         7724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1565267, whole genome shotgun sequence.
ACCESSION   DASTKS010000034 DASTKS010000000
VERSION     DASTKS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565267"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFQ35_00710"
     CDS             complement(<1..292)
                     /locus_tag="VFQ35_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9929168.1"
                     /translation="MRRELAALAFMAACSARPSPPPRPTMAAPTSNSAVVAPANVPHE
                     PTRRQELREHLFGVAVDDPYRWLEAGDDPEVIQWVERQNAATQAYLAKSPARG"
     gene            complement(292..1302)
                     /locus_tag="VFQ35_00715"
     CDS             complement(292..1302)
                     /locus_tag="VFQ35_00715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929169.1"
                     /translation="MSYSSPPRSAPRRSSPVSVRPSQLTGTVLGDRYKIHGYLRRGVG
                     SRVFLAEDLESHGSVVIKMLSPAAANDMERRMRLEQARAKLTPIQHGNLVDLLGVDET
                     DAGIPYLVMEALSGETLDESLRRVGALPLDLALVVARQAAAGLIALHDAGIVHGNVSP
                     ENLMLLGAPTELYGVKVLNYGIAAEFRDDQSEQSFDPVRTAYRAPEQLLDERADAESD
                     VYALGVVLLHSLCGKLPFQGADEAECLRAKLETNTLDKVWLDLGQDPRLETIIVNSTR
                     KHPRNRYRSMRALLDALDVIVGLSALDVRALPLTQSPDGYEPGTKRGRERLAAFMATR
                     TP"
     gene            complement(1424..2764)
                     /locus_tag="VFQ35_00720"
     CDS             complement(1424..2764)
                     /locus_tag="VFQ35_00720"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9929170.1"
                     /translation="MPKDTLTITDNRTGKTYELPIAEGTIRATDLRQIRVDEQDFGMM
                     TYDPGFMNTCSCRSAITYIDGDQGILRYRGYPVDQLAENASYLEVAYLLINGELPTQS
                     ELDEFTINVTHHTFVHENVRTFIDGFRYDAHPMGMLVATVGALSTFYPEAKHVLDSEI
                     RRNQMVRLIAKMPTLAAWSYRHSMGMPFVYPSNELSYSENFLYMMKRIAEPKFEIHPE
                     IVRALDVLFILHADHEQNCSTNVMRGIGSSRADPYVAVAGAAAALYGPLHGGANEAVL
                     RMLREIGNVSNVPAFIKEVKGGVGSRLMGFGHRVYKNYDPRARVIKRLADQVFDIVGK
                     KSPLLSVALELERIALSDDYFVQRKLYPNVDFYSGLIYEALKIPVDMFPVLFAIPRTA
                     GWLAQWEELLLDKDQKIARPRQIYTGYDERKFVSINDRQSSRTEPVKSSPRETD"
     gene            2886..4493
                     /locus_tag="VFQ35_00725"
     CDS             2886..4493
                     /locus_tag="VFQ35_00725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9929171.1"
                     /translation="MLSGVFRLWCAALPLGALALACQNRGASDDPALIKLKPESGGPK
                     LAGEDWSRVPVPAADGPVLAPVAMAVPVRAKPAPDGEIIGVLRVGSRIARSDAPVTLR
                     DCAGGWYAVRPVGFVCVGNDVTLKLDHPVARAIDVEPDRSKPLPYRYAFLRAVAPNYM
                     RIPNKDEQFRYEMRLDRHLKNWKKFREKWDALELGANDVPLDESGIPSGGFAPNARPM
                     DERERFGARGDDHVPWWLEGERRIPNIASFKVPAYAVISNRIKRHGGVALIGSFVGPE
                     AAQSRRFAITTDGRLLPADKLKADPGSAFHGYDIRNVGLPVAFVRARDANAYRAENNR
                     FLEAKGPEFRSLVALTGNVRVQGGERFVETRDGKWLESDQLGTVAKPSSLPGWVKKTT
                     KWIDVSLANQTLTLWEGDRPVYATLVSTGRDGMGEPGKTLSTPQGIFRIFQKHVTTTM
                     DSDVADHEFELRDVPWVMYFKGGYALHGAYWHDDFGRPRSHGCVNLAPIDARYVFSWS
                     SPNVPEHWQAAYAGENTEPGTIVNIHP"
     gene            complement(4490..5221)
                     /locus_tag="VFQ35_00730"
     CDS             complement(4490..5221)
                     /locus_tag="VFQ35_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929172.1"
                     /translation="MEIQRGRLAYRSRRLELRGAAGVLEIWFHLRDDALKDLRKVAFG
                     PYDWTQANATEVLCRLAIEGVETTHTSEQIARALPTWRYEQIMRVCAFVAELSVRSPA
                     LRAAYEALIVNYLEGDPVDAFELIAALARFDPRAVRQRYGDFLLQLSEGTGLVGRTAF
                     DDGHVVTGDDGKSLVAKSGPTYPLLEDFHRIRAAVLRYELFPEDERTRKRLAEWSVQH
                     PDESVRSELAALLQRRTSASRESTR"
     gene            complement(5245..5961)
                     /locus_tag="VFQ35_00735"
     CDS             complement(5245..5961)
                     /locus_tag="VFQ35_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929173.1"
                     /translation="MKRLSEELCSGDAAEGRLKELVRSAQPFRANPFRKRKLLLQMSR
                     ASGARTHAISRATRSAVVLAVLGSAGIAGASYGWLKHTAAGAAPALASQSVVSPPPTT
                     AAPRAPKPAPEVVAAPAPAPETAADKPVENTQVRRLERPLSNKARAKPEGEDPSEVLE
                     AIRALRKQGDAARAQVLLDQYLKTHPGGALSEDALVLSIEAALARHDPRATDYAKRYL
                     KSFPKGRFRSLAERVVASHP"
     gene            complement(5958..6572)
                     /locus_tag="VFQ35_00740"
     CDS             complement(5958..6572)
                     /locus_tag="VFQ35_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929174.1"
                     /translation="MALKRSLTHEKLALELSAPDASSTAAPAAAPLEFEAIYEQWFEE
                     VLRWIRALGGPEADRDDLVQDVFMVVHRRLPFFDGKNVGGWLYQIARRKVRDFRRLFW
                     VRHLFGQSVPLTENTAQSVGSPYDSLETKEQGELLDELLTSLKPDQRAAFVLFEIEGY
                     SGEEIARLQGVPLNTVWARIHKARKKLQGRIAQRERAARVRRGE"
     gene            6712..7701
                     /locus_tag="VFQ35_00745"
     CDS             6712..7701
                     /locus_tag="VFQ35_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929175.1"
                     /translation="MWSDALRRAATFGALTLSVTTLARAQPASGALEVSGYGTCPEPS
                     TVRAAILQLTSASSRAALPAGSAVFVNDQGESFRVSITTEGSTAERTYSDPARSCERR
                     ARFAAVFAIVTLMPPDLGAEAEPEPAETPPPVAPEPEPPSPAMPTFGAPVSPRPVPSA
                     PWLRVALTGVAEQALDPEGHLALRGFGAELLSAIGRAWLTPSFSLSIAPKRELTLPDT
                     RCELTRVEAALGARASEPIGAYSLGADLGLAAAWSRVRGGELAHPAVGTAVDLGLRAR
                     GSVALSSARISPLLALEAAMFPFSRELRAVPQGELGRLPLLWLGVALGIQAGF"
BASE COUNT         1267 a         2467 c         2570 g         1420 t
ORIGIN      
        1 cgccgcgcgc gggcgacttt gcgaggtacg cctgggtggc ggcgttttgg cgctcgaccc
       61 actgaatgac ctcggggtca tcgcccgcct ccaaccaacg atacggatcg tcgaccgcca
      121 cgccgaaaag gtgctcgcgc agctcttgtc gtcgcgtcgg ctcgtgcggc acgtttgcgg
      181 gcgccacgac cgctgagttc gaggtcggcg ccgccatcgt agggcgaggc ggcggcgacg
      241 gacgagcgct gcacgccgcc atgaacgcga gcgcagcgag ctcgcgccgc atcacggagt
      301 ccgggtggcc atgaacgcgg ccagccgctc tcggcctcgc ttcgttccgg gctcgtaccc
      361 gtccggagac tgcgtcagcg ggagagcgcg gacgtcgagc gcagatagcc ccacgatgac
      421 gtcgagcgcg tcgagcagcg cgcgcatcga ccgatagcgg ttgcgcgggt gtttgcgggt
      481 ggaattgacg atgatcgttt cgaggcgagg atcttgcccc aaatcgagcc acaccttgtc
      541 gagcgtattg gtctcgagct tcgcgcgcag gcactcggct tcgtcggctc cttgaaatgg
      601 aagcttcccg cagaggctgt ggagcaggac gacgccgagc gcgtacacgt cgctctcggc
      661 gtccgcgcgc tcgtcgagga gctgctccgg cgcgcgatac gccgtgcgca cggggtcgaa
      721 gctttgctca gattggtcat ccctgaactc ggcggcgata ccgtagttca gcaccttgac
      781 gccgtacagc tccgtcggcg cgccgagcag catcaggttc tctggcgaga cattgccatg
      841 aacgatgcca gcgtcgtgca gcgcgatgag ccccgccgcc gcttggcgcg ctaccaccaa
      901 ggccaagtcg agcggcaacg caccgacgcg gcgcagcgac tcgtcgaggg tctcaccgct
      961 cagcgcctcc atcacgaggt acggaatgcc cgcgtcggtc tcgtcgacgc cgagcaggtc
     1021 cacgaggttg ccgtgctgaa tcggagtcag cttggcacgc gcctgctcga gccgcattcg
     1081 acgctccatg tcgttcgcgg ccgccggcga aagcatcttg atcacgacgg agccgtggct
     1141 ctccagatct tcggcgagga agacgcgcga gccgacgccg cgccgcaagt agccgtggat
     1201 cttgtagcgg tcgccgagca ccgttccagt cagctgcgac gggcgaacgc tgacgggcga
     1261 agagcgacgc ggagccgaac gcggcggtga agaataactc atcccctctt cctcgtgagc
     1321 gacctcgcga actcgggcgg ccagcggctg accgcaatga aaagggcccc ggcgcgagaa
     1381 cgaagtcgct cgtcctgctc gcagcggggc ccagggcctg cgctcagtcg gtctcgcgcg
     1441 gcgagctctt gacgggctcg gtgcggctgc tctgccgatc gttgatgctg acgaacttgc
     1501 gctcgtcgta acccgtgtag atctggcgcg ggcgggcgat cttctgatcc ttgtcgagca
     1561 agagctcctc ccactgcgcg agccagcccg cggtgcgggg aatcgcgaac agcaccggga
     1621 acatgtcgac cgggatcttg agggcttcgt aaatgaggcc cgaatagaag tcgacgttcg
     1681 gatagagctt gcgctgcacg aagtagtcgt cggagagcgc gatccgctcg agctcgaggg
     1741 cgacggagag cagcgggctc ttcttgccga cgatgtcgaa gacctgatcg gcgaggcgct
     1801 tgatgacgcg agcgcgcgga tcgtagttct tgtagacgcg gtgtccgaag cccatcagac
     1861 ggcttccgac gccgcccttc acctctttga tgaaggcggg cacgttgctc acgttgccga
     1921 tctcgcggag catgcgcagc acggcctcgt tcgcgccgcc gtgcagcggg ccgtagagcg
     1981 cggctgcggc gccggcgacc gcgacgtacg gatcggcgcg tgagctgccg ataccgcgca
     2041 tcacgttggt cgagcagttc tgctcgtggt cggcgtgcaa gatgaagagc acgtcgaggg
     2101 cccgcacgat ctcggggtgg atctcgaact tgggctcggc gatccgcttc atcatgtaga
     2161 ggaagttttc ggagtagctg agctcgttgc tcggatagac gaacggcatg cccatgctgt
     2221 ggcggtagct ccacgccgcg agcgtcggca tcttcgcgat gagccgcacc atttggttgc
     2281 ggcggatctc gctgtcgagc acgtgcttcg cttcggggta gaacgtcgac agcgcgccca
     2341 cggtcgcgac cagcatgccc atcgggtggg cgtcgtaacg aaagccgtcg atgaacgtgc
     2401 gcacgttctc gtgcacgaac gtgtggtggg tgacgttgat cgtgaactcg tcgagctcgc
     2461 tctgcgtggg gagctctccg ttgatgagca ggtacgccac ctcgaggtag ctggcgtttt
     2521 ctgccagttg atccaccgga tagccgcgat accgcaggat gccctgatcg ccgtcgatgt
     2581 acgtgatcgc cgagcggcac gagcacgtat tcatgaatcc gggatcgtaa gtcatcatcc
     2641 cgaagtcctg ttcgtcgacg cggatctgtc ggagatcggt cgcgcgaatc gttccttcgg
     2701 cgattgggag ttcgtaggtc ttgccggtgc ggttatcggt gattgttagg gtgtccttgg
     2761 gcatcgctct gtccggagtg ggggaccggg atactttccc gaagggtttg gccccgcaag
     2821 cccctcactg aacctttgat ggttttttgg cccgaccatt cggctttcgg catgaagatc
     2881 ccgccatgct ctccggcgtg tttcgtcttt ggtgcgccgc cctgcctctg ggtgctttgg
     2941 ccctcgcttg tcaaaatcga ggcgcttctg atgatcctgc gctcatcaag ctgaagccgg
     3001 aaagcggcgg tccgaagctg gccggcgaag attggtcccg ggtgccagtg ccagcggccg
     3061 atggtcctgt gctcgcgccg gtcgcgatgg cggtcccagt tcgcgcaaaa cccgctccgg
     3121 acggggaaat catcggcgtt ttacgcgtag gttcgcgcat cgcgaggtcc gatgctccgg
     3181 tcacactgcg cgattgcgct ggtggttggt acgccgtgcg accggtaggt ttcgtatgtg
     3241 tcgggaacga cgtcaccctc aagctcgatc atcccgtcgc gcgggccatc gacgtggagc
     3301 cggatcgcag caagccattg ccttatcgct atgcattctt gcgggccgtt gcgccgaact
     3361 acatgcgcat ccccaataaa gacgagcagt ttcgctacga aatgcggctc gaccggcatc
     3421 tgaagaactg gaagaaattt cgcgagaagt gggacgccct ggagctcggc gccaacgacg
     3481 tgccgctcga tgaaagcggg atcccgagcg gtgggttcgc gcccaacgcg cgccccatgg
     3541 acgaacgcga gcgtttcggc gctcgaggtg acgaccacgt cccttggtgg ctcgagggcg
     3601 agcgacgcat tcccaacatc gcaagcttca aggtgcccgc ctacgccgtg atctcgaatc
     3661 gcatcaagcg ccacggtgga gtcgcgctca tcggtagctt cgtcgggccc gaagccgcgc
     3721 agagccgtcg gtttgctatc acgaccgatg gtcgtctctt gcccgccgac aagctgaagg
     3781 cggatcccgg ctcggccttc catggctacg acatccgcaa cgtgggactc ccggtcgcgt
     3841 tcgttcgcgc gcgcgacgcc aacgcgtatc gcgccgagaa caaccgcttc ctcgaggcga
     3901 agggacctga gtttcgttca ctcgtcgccc tcaccggcaa cgttcgagtg cagggcggcg
     3961 agcgcttcgt cgaaacccgc gacggcaagt ggctggagag cgatcagctc ggaaccgtcg
     4021 cgaagccgag ctcgctccca ggttgggtga agaagacgac gaagtggatc gacgtttcgc
     4081 tcgcgaacca gactttgacg ctgtgggaag gtgaccggcc cgtttacgcg acgctcgtct
     4141 cgaccggccg cgatggaatg ggtgagccag gcaagacgct cagcactccg caaggcatct
     4201 tccgcatatt ccaaaagcac gtcacaacga cgatggactc ggacgtcgcc gaccacgagt
     4261 tcgagctccg cgacgtgcca tgggtcatgt acttcaaagg tggctacgcg ctccacggcg
     4321 cttactggca cgacgacttc ggtcgacctc gctcgcacgg ttgcgtcaac ctcgcgccga
     4381 tcgacgcgcg ctacgtgttc tcgtggtcgt ctcccaacgt ccccgagcac tggcaggcgg
     4441 cgtacgcggg tgagaacacc gagcccggca cgatcgtgaa cattcaccct tagcgggtgc
     4501 tctctcggct tgcgctcgtg cggcgctgta gcaacgccgc aagctcactg cggacgcttt
     4561 cgtcgggatg ctgcaccgac cactccgcga ggcgtttgcg ggtacgctcg tcctccggaa
     4621 agagctcgta gcgcaagacg gcggcgcgga tgcggtggaa gtcctcgagc agggggtagg
     4681 tgggcccgct cttcgcgacc aagctctttc cgtcgtcgcc cgttacgacg tggccgtcat
     4741 cgaaggcggt gcgtccgacg agcccggtgc cttcggacaa ttgcagcagg aagtctccat
     4801 accgctgcct gaccgcccgt ggatcgaagc gggcgagcgc cgcgatgagc tcgaaggcgt
     4861 cgacggggtc tccttcgagg tagtttacga tcaaagcctc gtacgcggct cgtagtgcgg
     4921 gggagcgcac actgagctcg gcgacgaagg cgcacactcg catgatttgt tcgtagcgcc
     4981 acgtgggcag cgcccgcgcg atctgttcgg aggtgtgcgt cgtctcgacg ccctcgatgg
     5041 ccaggcggca gagcacttct gtcgcgttcg cttgggtcca gtcgtaaggg ccgaacgcca
     5101 ccttgcgtag gtccttcagc gcgtcgtcgc ggagatgaaa ccagatctcg agaactccgg
     5161 ctgcaccccg aagctcgagg cgacgcgagc gatatgcgag ccgacctctt tggatctcca
     5221 aaacacgcct cgagcgtcga gcagctaggg gtgcgacgca acgacgcgtt cggcgagtga
     5281 acgaaagcgc cctttgggaa agctcttcag atagcgtttg gcgtagtcgg tcgcgcgagg
     5341 gtcgtgccgg gcgagagcgg cttcaatcga gagcacgagc gcgtcttcgc tgagcgcgcc
     5401 accaggatgc gtcttcaagt attggtccag gaggacctgg gcgcgcgccg cgtcgccctg
     5461 cttgcgcagc gcgcgaattg cctcgagcac ctccgaggga tcctctccct ccggcttggc
     5521 ccgtgccttg ttcgatagcg gccgctccag gcgacgaacc tgtgtgttct ctaccggttt
     5581 gtcagcggcg gtttctggcg cgggggctgg cgcggcgacc acttcgggcg ctggttttgg
     5641 cgcgcgtggc gctgcggtcg tcggcggtgg cgaaacgacg ctctgactcg ccagcgcggg
     5701 agcagcaccg gcagcggtat gcttcaacca accataggat gcgcccgcga tccccgcgct
     5761 gcccaagacc gcgagtacga ccgccgaccg cgtcgctctc gagatagcgt gtgtgcgcgc
     5821 gcccgaagcc cgcgacatct ggagcaacag cttgcgcttg cggaacggat tcgcgcggaa
     5881 cggttgagcg ctccgcacga gctctttgag ccgaccctcg gccgcgtctc cagagcagag
     5941 ttcttcgctc aagcgcttca ctcgcccctc cgtactcgcg cggcccgctc gcgttgggcg
     6001 atgcgccctt ggagcttctt gcgtgctttg tggatgcggg cccagacggt gttgagcggc
     6061 acgccttgca gccgcgcgat ctcctctccg ctgtaacctt cgatttcgaa gagcacgaat
     6121 gccgcccgct ggtctggctt caggctggtg aggagctcgt cgaggagctc gccctgttcc
     6181 ttggtttcga gcgaatcgta aggcgagccg acgctttgcg ctgtgttttc ggtgagggga
     6241 acgctttggc cgaagaggtg ccgtacccaa aacaaccgac gaaagtcgcg gacttttcgg
     6301 cgcgcgattt ggtagagcca gcctcccacg ttcttaccgt cgaagaacgg caggcgtcgg
     6361 tggacgacca tgaacacgtc ttgcaccaga tcgtcacggt ccgcctctgg cccgccgaga
     6421 gcacgaatcc agcgcaacac ctcctcgaac cattgctcgt agatcgcctc gaattcgagc
     6481 ggtgcggccg caggcgcagc cgtgctcgac gcatcgggtg cgctgagctc gagagcgagc
     6541 ttttcatggg ttagtgatcg tttaagggcc acgagccgca gaaaccttgc cggaaattct
     6601 taaaacgcga gaaccgagcc gaaaccgacg cacgcgagcc ggctagaacg gaacggccgg
     6661 cgtcgattat tcggggacac gagcagccga acctgatgta ttcacgggcg cgtgtggtcc
     6721 gatgccctgc gaagagctgc caccttcggc gcgcttaccc tgtcggttac cacgttggct
     6781 cgggcacagc cagcgtcagg ggcactcgag gtcagcggat acggaacatg tcccgagccc
     6841 tcgacggtgc gcgctgcaat tttgcagctc acctcggcga gtagtcgtgc ggcgttgccc
     6901 gcgggatccg cagtgttcgt caacgatcaa ggcgagagct ttcgcgtatc gatcacgacc
     6961 gaaggcagca ccgccgagcg cacctactcc gaccccgcgc ggagctgcga gcgcagggcg
     7021 cgctttgcgg cggtgttcgc gattgtcacc ctgatgccgc cggatctcgg cgccgaagcc
     7081 gagccggagc cagctgagac accgcctccg gtcgctcccg agccggagcc cccgtcaccc
     7141 gcaatgccca cgtttggtgc tccggtctcg ccgcggcccg taccctctgc gccgtggttg
     7201 cgagtggctc tgaccggggt cgccgagcaa gcgctcgatc ccgaagggca cctcgcgctg
     7261 cgtggcttcg gggcggagct cctcagcgcg atcggaagag cttggttgac gccgtcgttc
     7321 tcgctatcta tcgcgcccaa gcgtgagctt acgctgcccg atacgcgctg tgagctaacg
     7381 cgggtcgaag ctgccctcgg cgcgcgtgcg agcgaaccga ttggcgccta ttcgctcggc
     7441 gcagatctcg gccttgccgc cgcgtggtcc cgtgtgcgcg gaggagagct cgcacatccc
     7501 gcggtgggga cggcggtcga tctcgggctc cgcgcgcggg gcagcgtggc gctgtctagc
     7561 gcgcgtatca gtccgctcct cgcgttggaa gcggccatgt tcccgttctc gcgcgagctc
     7621 cgcgccgttc cccaaggtga gctcggccgc ctcccgttgc tctggctcgg cgtggccctc
     7681 ggaatccaag cggggttctg agcggcgtaa cggggcgctt attc
//
LOCUS       DASTKS010000035         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1565332, whole genome shotgun sequence.
ACCESSION   DASTKS010000035 DASTKS010000000
VERSION     DASTKS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565332"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VFQ35_00750"
     CDS             <1..1444
                     /locus_tag="VFQ35_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET9929176.1"
                     /translation="LDLSLMGEFLEHLLKLPIGFFQTRTQGDLVRRVQGNMALRDLFT
                     SRSVSALLDTFLLFAYAGLMLAYHVRLGVVVIAIALVRVAILLAMRSRIEQAATTELA
                     AAGREGGALVEALAVPEVTKAFGTEGRMLAHYSDKLVYRVNSELARRRLTNGVGYLML
                     FFDGASKAAIVWFGGREVLADRMTIGVLSAFMMLQLLFAGPLESIVNTFNDLQFFGRQ
                     LQRLDDVMETPREPSGERDPGRLQGEIALEDVEFRYGPSGRNVIDGVSIKVRRGEKVA
                     LVGRSGAGKSTLARLLLGMHWPTKGRVRIDGVDLKELDVAKVRAQFGVVLQEPFLFDD
                     TVRANIALNDEELPLENVREAARVACIEDAVDGMAEGFETRVGQRGIRLSGGQRQRIA
                     VARAIAHRPAVLLLDEATSSLDRDTERSLHANLAGIACTRIVIAHRLATVQDADRIFV
                     LDGGRIVDQGNYAELSERPGLFRDLVRALV"
     gene            1437..3482
                     /locus_tag="VFQ35_00755"
     CDS             1437..3482
                     /locus_tag="VFQ35_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9929177.1"
                     /translation="MSNAVAVFPGAHSSSDAVVALWRLMRARRIDVTKQQLREACGGV
                     VSASGFADELRTRGCPARVVRLREQSDLAHLELPTLVMLRDSGAALVEQVKHDRVLLR
                     FSDGGRKKVRFAEFSEITSGEALDLSEGLPSRTGYLRRLVAALGAHPVPLVQIAVVTL
                     LMHVANAGLPLLLRTAVDDALPNGAGNTLRLAAIAMLAVAASHAWLGWFRERALQFLN
                     ARGVSALARGILEHLVHLPLAYFQKTSLGNSLQAMAAGEQIARLVTDLALVPFVDGAL
                     SVVNFGVLFLLSPTAGAIVLAANIVLLFVAVVLGKREATLQAEELAAQAKQNGYLVEL
                     LSAAPTLKAAGAEMRAVRRWRELLASERWPSLRRQRVRLGVELTAEWLGHSVVALLLV
                     WGGKQSLDGHLSIGTLVAVLQLGRTLGETNYRLAEVCVKIWIAKPHSAILRELLQHTP
                     TAALRSGAGARSSAGDAIVLHDVWFRHGPELPWVVQGFHLRIGRGEHYKLTGPSGSGK
                     TTLLRLIAGLYQPERGAVMVHGVDAMASRRSVAYVPQDSYLFQGSILDNMRVLSGSAS
                     RESLMKAAKLTGLEAFVNTLPMSYETLLPPGGTNLSGGQRQLIVLTACVASSRPVLVL
                     DEAMSQLDRLTQAALLKAALFSGKTVVSVVHEPDGASASTVVTLDARDESRPTDAGE"
     gene            complement(3423..>4398)
                     /locus_tag="VFQ35_00760"
     CDS             complement(3423..>4398)
                     /locus_tag="VFQ35_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9929178.1"
                     /translation="RAGEDVAAADASTAELNAVLDAAVRYLTVAEDETSVTAFNAALE
                     TRLELQRVTHELVAQGLRPPIDEGRVQVEVDVARFDLMVAGYRLQQDTVELSTALGLD
                     PTTELRVEPIGDALVSVDDDPKRAAVAAEASRPELQAARAQLAQSQADIESARTARLP
                     TLAVLAQGQAGHTEVLSGIGVAGRNESGQVGISLSIPVLDPVRSAQRTVAEKASVVAA
                     ERLRAARLAIRNDAVRAALAVRGSRALLQQAERVRSGASANAATARERYARGMTPVID
                     ELIEAQARERSATSDAIRARLVLQVACARLLASIGGAALVASVQGDHR"
BASE COUNT          656 a         1431 c         1508 g          803 t
ORIGIN      
        1 cctcgatttg agcctgatgg gggagttctt ggagcacttg ctcaagctgc cgattgggtt
       61 cttccagacc cgcacccaag gagacctcgt tcggcgcgta cagggcaaca tggccctgcg
      121 cgatctgttc actagccgct ccgtttcggc gctgctcgat accttcttgc tgttcgctta
      181 cgcgggcctg atgctcgcct atcacgtgcg cctcggggtc gtcgtgatcg cgatcgcgct
      241 cgttcgcgtc gccatcctgc tcgcgatgcg gagccgaatc gagcaagccg ccactaccga
      301 actcgcggcg gccgggagag aaggcggcgc cctcgtcgaa gcactcgcgg taccggaggt
      361 gacgaaggcc ttcggcaccg aggggagaat gctcgcgcac tactcggaca agctcgttta
      421 tcgggtgaac tcggagctcg cgcggcgccg cctgacgaac ggggtcggct acctgatgct
      481 cttcttcgat ggcgcgtcca aggccgccat cgtttggttc ggcgggcgcg aggtgctcgc
      541 ggatcgcatg accatcggcg tgctgagcgc attcatgatg ctgcagctct tgttcgcggg
      601 cccgctcgag tcgatcgtca acacgttcaa cgatctacag ttcttcggcc ggcagcttca
      661 gcggctcgac gacgtgatgg agactccgcg agagccgagc ggcgaacgag acccgggacg
      721 actgcagggt gagatcgcgc tcgaagacgt cgagtttcgc tacgggccga gcgggcgcaa
      781 cgtcatcgac ggcgtcagca tcaaggttcg gcgcggggag aaggtcgccc tggtcgggcg
      841 ctcaggcgct ggcaaatcga cgttggcgcg cctcttgctc ggcatgcact ggccgacgaa
      901 gggccgtgtt cgcatcgatg gcgtcgactt gaaagagctc gacgtcgcca aggtgcgggc
      961 tcaattcggg gtcgtgctgc aggagccctt tctcttcgac gacaccgtgc gcgccaacat
     1021 cgcgctcaac gacgaggagt tgccgctcga aaacgtgcgt gaagcagcgc gcgtggcttg
     1081 catcgaagac gccgtcgacg gcatggctga aggcttcgag actcgcgttg gccaacgtgg
     1141 gattcgtctt tcgggtgggc aacggcagcg catcgccgtc gcgcgcgcca tcgcgcaccg
     1201 ccccgcggtg ctgttgctcg acgaagcaac gagctcgctg gatcgcgaca ccgagcgctc
     1261 cctccacgcg aacttggctg gcatcgcgtg cactcgcatc gtcatcgcgc accggttggc
     1321 gacggtccaa gatgcagacc gcatcttcgt gttggacggc ggtcgcatcg tcgaccaggg
     1381 caactacgcg gagctctccg agcgcccggg gctgtttcga gatctcgtga gggcgcttgt
     1441 ctaacgcggt cgccgtcttt cccggcgctc actcatcgag cgacgccgtc gtcgcgctct
     1501 ggcgcctgat gcgggcgcgg cgcatcgacg tgacgaagca gcagcttcgg gaggcgtgcg
     1561 ggggcgtcgt gtcggcgtcc ggcttcgccg acgagctccg aacccggggt tgcccggctc
     1621 gcgtcgtccg gctgcgcgag caaagcgatc tcgcgcacct ggagcttccg acgctcgtca
     1681 tgttgcgcga cagcggggcg gcgctggtcg agcaggtgaa acacgaccgg gtactactcc
     1741 gcttctccga cggcggtcga aagaaggtcc gtttcgcgga gttctccgaa atcacgtcgg
     1801 gcgaggcgct cgacttgtcg gaggggcttc cgtcgcgcac aggctacctc cggcggctcg
     1861 tggccgcgtt gggcgcgcat ccggtgccgc tcgtccagat tgccgtcgtg acgctcttga
     1921 tgcacgtggc gaacgcgggt ttgcccttgc tcctccgtac cgcggtcgac gacgccttac
     1981 cgaacggtgc cggaaacacg ttgcgtctcg ccgcgatcgc gatgctcgcc gtggccgcga
     2041 gccacgcttg gttggggtgg tttcgggaac gcgcgctgca attcctgaat gcacgcggcg
     2101 tgagtgcgct cgcgcgcggc attctcgaac acctggttca cctgccgctc gcctacttcc
     2161 agaagacttc gctcggtaac tcgctccaag cgatggcggc gggcgagcag atcgcgcgac
     2221 tcgtcacgga cttggcgctg gtgccgttcg tcgacggagc gctctcggta gtaaacttcg
     2281 gcgtcttgtt cctgctctca ccgacggcgg gagcgatcgt tctcgcggcc aatatcgtgc
     2341 tcctgttcgt ggcggtcgtc ctcggcaagc gtgaggcgac gcttcaagcc gaagagctcg
     2401 cggcgcaggc gaagcaaaat ggctacttgg tcgagttgct ctcggcggcc cccaccttga
     2461 aggccgccgg tgccgagatg cgcgcggtgc gaaggtggcg agagctcctc gcgagcgagc
     2521 gctggccgag tctgcgcagg caacgggtgc gcctcggggt cgagctcacc gcggaatggc
     2581 tcgggcactc cgtggtcgcg ctcctgctcg tttggggcgg caagcaaagc ttggatggtc
     2641 acctttcgat cggcaccttg gtcgcggtgc tgcagctcgg acggacgttg ggggaaacca
     2701 attatcgcct cgcggaagtc tgcgtgaaga tttggatcgc caaaccgcac tcggcgattt
     2761 tgcgcgagct cctgcaacat acgccgacgg ccgcgctccg gtcgggcgcc ggtgcgcgaa
     2821 gctcggccgg cgacgcgatc gtgctgcacg acgtgtggtt ccggcacggc cctgagttgc
     2881 cctgggtggt gcaggggttt cacttgcgca tcggtcgtgg cgagcactac aagctcaccg
     2941 gcccctccgg ctcgggaaaa acgacgctct tgcgcctgat cgcgggcctc tatcagccgg
     3001 agcgcggagc cgtgatggtc cacggggtcg acgcgatggc gtcacggcgg tcggttgctt
     3061 acgtccccca ggactcttac ctgtttcagg gctcgatcct cgacaacatg agagtattgt
     3121 cgggcagcgc ctcccgcgag agcctcatga aggcggcgaa gctaacgggc ctcgaagcat
     3181 tcgtgaacac gctgccgatg tcgtacgaaa ctctcttgcc tccaggcggg acgaacttgt
     3241 cgggtggcca acggcagctc atcgtgctca ccgcttgtgt cgcgtcgagt cgaccggtgc
     3301 tcgtgctcga cgaggcgatg tctcaattgg atcggctcac gcaggcggcg ctcctgaagg
     3361 cggcgctctt ctcgggcaaa acggtcgttt ccgtggtgca cgagcccgac ggcgctagcg
     3421 cctcaacggt ggtcaccctg gacgctcgcg acgagagccg ccccaccgat gctggcgagt
     3481 agacgagcgc aggcgacttg caagacgagg cgcgctcgga tggcatcgct cgtcgcgctg
     3541 cgttcgcgcg cctgcgcttc gatgagctcg tcgatgaccg gcgtcattcc gcgcgcgtag
     3601 cgctcgcgag ccgttgccgc gttggcgctg gctcccgagc gcactcgctc cgcctgctgc
     3661 aggagcgccc gcgagccgcg caccgcgagt gcggctcgaa ccgcgtcgtt tcggatggcg
     3721 agcctcgccg cgcgcagccg ttccgccgcc acgacgctgg ccttttcggc gacggtgcgc
     3781 tgggcactgc gcacggggtc gaggacgggg atgctcaagc tgatgcccac ctggccgctc
     3841 tcgtttctgc ccgcaacgcc gatcccggat agcacctccg tatgaccggc ttggccttgt
     3901 gccagtaccg cgagcgtcgg cagccgcgcc gtacgcgccg actcgatgtc ggcctgactc
     3961 tgcgccaatt gcgcccgcgc cgcttgaagt tcagggcgcg atgcctcggc cgcgaccgcg
     4021 gcccgtttgg gatcgtcgtc gaccgagacg agggcatcgc cgatcggttc gacccgaagc
     4081 tcggtcgtcg gatccaggcc gagcgcggtc gaaagctcta ccgtgtcttg ctggagtcga
     4141 tagccggcca ccatcaggtc gaagcgcgca acgtccacct cgacctgaac gcggccctcg
     4201 tcgatgggcg gacgtaggcc ctgggcgacg agctcgtgag tcacgcgttg tagttcgagg
     4261 cgcgtctcga gggcggcatt gaaagcggtg accgaggtct cgtcctcggc gacggtgagg
     4321 tacctcacgg ccgcatccaa gacggcgttg agctcggcgg tcgaggcgtc ggcggcggcg
     4381 acgtcctcgc cagcgcgg
//
LOCUS       DASTKS010000036         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_4960,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000036 DASTKS010000000
VERSION     DASTKS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4960"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFQ35_00765"
     CDS             <1..669
                     /locus_tag="VFQ35_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929179.1"
                     /translation="WGMTHAGVTGSQCSWSTCNGDSGISWGTEYDWRGGPAGQVTSYA
                     AVILGWHFSTIPADSGLPVMIGDHRAIDCSWSFNVKPTAPSTQNVAYDIWLSPDPAPT
                     GSTRPTDEVMVWLYRSGPASPIGGTVVDTLSLPSGDFALSEGRNGPSGDTFVAGVNGW
                     NVHSFVRTGSQPCSDLNLADFFDFLIEKYGVDPAKYVIGIEAGTEVFSGAGTLTTDHF
                     SCNL"
     gene            complement(799..2028)
                     /locus_tag="VFQ35_00770"
     CDS             complement(799..2028)
                     /locus_tag="VFQ35_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9929180.1"
                     /translation="MSSAEGEWLGAKEASALLGVRRETLYAYASRGLVRVRRVGSARV
                     YARADLEALRARSQARRGHAAVAAGALRFGEPVLDTRVSSIGADGPRYRGRSAVELAQ
                     QDVSAERVAELLWTEVLPEHEPVWPRAEKVELDALRERQRVELSPAGAMLLVLANTPH
                     DEGAASRSREDELDSARRLLRLVAAAPALGLPPRWLDKALSARTLAAALLSSLAKRSS
                     EPAEAALNQALTLIADHELNASTFVARVAASAGADMPRSLAAAFATLTGARHGGVCDR
                     LDTVLDGFSRPEQAIAWVRAELAQRRAVPGFGHPLYASGDPRAAPLLRASLELGADVP
                     RVRAVHVAIDAMALAGGEPATVDLGLVAIAAALGLRSGSAAAIFALGRALGYVAHVLE
                     QREQGFLLRPRAHYVGI"
     gene            2129..3280
                     /locus_tag="VFQ35_00775"
     CDS             2129..3280
                     /locus_tag="VFQ35_00775"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9929181.1"
                     /translation="MTSNFKGFSDSKASSLGLDDVTFANTEISHIDGAQGELWIRGYP
                     IEALSGHASFEAVCGLLWDGALPDAEREAEIRDSLGRARTAAYERRSRWLEPALRHSE
                     PMAGLRAGLALTEAPSDEFSEACLVTATAGLFLAAWQSRVRGSELSEPSPSESHARDL
                     VRLGNGSGDEERARALDAYLVTVAEHGMNASTFTARVVASTESDTVSAVTAAVGALKG
                     RLHGGAPGPVLDMLDAVGTEDRARAYLEAELAANRRIMGMGHRIYRVRDPRALVLERA
                     TQRLAGAQHTPRLNLARHVERVATELLAERHPERRLCANVEFFTAVLLEAVSVPRELF
                     TVTFAAARVAGYCAHIAEQRRRGRIVRPASRYVGPRPSGEAPTVLAVSA"
     gene            3462..>4129
                     /locus_tag="VFQ35_00780"
     CDS             3462..>4129
                     /locus_tag="VFQ35_00780"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9929182.1"
                     /translation="MTDFKQIQSLLGDKASYLLEHQSKTVSKDKLHLPSPSFVDDVWA
                     ASDRSPRVLRALQTLFNTGRLANTGYLSILPVDQGIEHSAGASFAKNPAYFDPENIVK
                     LAIEGGCSAVASTFGVLGICARKYAHKIPFMVKLNHNELLSYPNTYDQTLFGTVRQCF
                     DMGAQGVGATVYWGAPESRRELHEVSEAFAEAHRLGMFTVLWCYVRNSAFKTAQGDHS
                     LSADL"
BASE COUNT          703 a         1429 c         1354 g          643 t
ORIGIN      
        1 tggggcatga cccatgctgg cgtcacaggc agccagtgct cgtggagcac ttgcaacggc
       61 gacagcggca tctcctgggg caccgagtac gactggcgcg gaggtcccgc ggggcaggtc
      121 acctcttacg cggccgtcat cttgggatgg cacttcagca ccatcccggc ggattctggg
      181 ttgccggtga tgatcggcga ccaccgtgcg atcgactgca gctggagctt caacgtgaag
      241 ccgaccgccc catctacgca aaacgtcgcg tacgatatct ggctctcgcc cgatccggct
      301 ccgactggtt cgacgaggcc gacggacgaa gtcatggtct ggctctatcg gtccggcccg
      361 gcttccccca tcggcggcac cgtggtcgac acactctcac tgccgagcgg ggactttgcg
      421 ctgagcgagg ggcgcaacgg cccttccgga gacaccttcg tcgcaggcgt gaacggctgg
      481 aacgtgcact cgttcgtacg gactggctct caaccgtgca gcgatttgaa cctcgccgac
      541 ttcttcgatt tcctaatcga gaagtacggc gtcgatccag cgaagtacgt catcggcatc
      601 gaggcgggga ccgaagtctt ctccggtgct ggcacgctga cgacggacca cttctcgtgt
      661 aacctctagt ttcggcccgt cgcgagaacc gatacgtcgc gttcgaaaac acgctgagcg
      721 gcgcggtcga agagactccg cggctcacgc tgctactcgc cgagtcaagc cgagccaacg
      781 tcggctcgcg cgaaaagctc agatcccgac gtagtgtgcg cgcggccgca acaagaatcc
      841 ttgctcgcgc tgctcgagta cgtgcgcgac gtagccgagc gcgcggccca gcgcgaagat
      901 ggccgccgcg ctcccggaac gaaggcccag agcggctgcg attgcgacca ggccgaggtc
      961 gacggtggct ggttcgcctc cggccagcgc catcgcgtcg atcgcgacgt gcacggcgcg
     1021 cacacgcggc acatcagcgc cgagctcgag gctcgcgcgc agcaacggcg ccgcgcgagg
     1081 atccccagac gcatacagcg gatgaccgaa gccggggacg gcgcgccgtt gcgcgagttc
     1141 ggctcgcacc caggcgatgg cttgctcagg acgtgagaag ccgtcgagca ccgtgtcgag
     1201 ccgatcgcag acgccaccgt gtcgcgcgcc ggtgagggtc gcaaacgcgg ccgccagcga
     1261 gcgcggcatg tcggcccccg cgctcgccgc cacccgagcg acgaacgtgg aggcgttgag
     1321 ttcgtggtcg gcgatgagcg tgagcgcctg attcagcgcc gcctcggctg gttcgctcga
     1381 acgctttgca aggctcgaga gcagcgccgc ggcgagggtg cgcgcggaga gcgccttgtc
     1441 gagccaacgc ggcggtaacc cgagcgcggg cgccgccgcg accaagcgaa gcagccggcg
     1501 cgcagaatcg agctcgtctt cgcgcgagcg cgacgcagcg ccctcgtcgt gaggagtgtt
     1561 cgccaacacg agcaacatcg ctcccgccgg tgatagctcg acgcgctggc gctcacgaag
     1621 cgcgtcgagt tcgaccttct ccgcgcgtgg ccaaaccggt tcgtgctccg gcaacacctc
     1681 cgtccaaagt agctcggcga cgcgctccgc gctcacgtcc tgctgcgcga gctcgaccgc
     1741 cgaacgaccc cggtaccgcg gcccgtcagc gccgatcgag ctcacccgag tatcgagcac
     1801 cggctctccg aagcgcaacg cgccagctgc gacggccgca tgcccacgac gcgcctggct
     1861 tcgagccctc agcgcttcga gatcggctcg cgcgtacaca cgcgcagaac cgacgcgccg
     1921 gacacgtacg agcccgcggc tcgcgtaggc gtacagcgtc tcgcgacgga cgccgagtag
     1981 cgcgctggcc tccttggcgc cgagccactc gccttctgca gagctcattc cagcacattg
     2041 attgatgaat caacgttgat caatgcgctg cgacacctaa tttgaatcgt gcgaacgccg
     2101 acgcgagcgg cgcgccggga agaccgacat gactagcaat ttcaaaggct tcagcgacag
     2161 caaggcgagc agcctaggcc tcgacgacgt caccttcgcc aacacggaga tcagccatat
     2221 cgacggcgcc cagggagagc tctggatccg cggctacccg atcgaagctc tgagcgggca
     2281 cgcctcgttc gaagccgtct gcggtctact ttgggatggc gcgctgcccg acgcagagcg
     2341 tgaagcggag atccgcgaca gcctcggccg ggcgcgcacg gctgcttacg agcgtcgctc
     2401 gcgttggctc gagccggcgc tgaggcacag tgagccgatg gcggggttgc gcgccggtct
     2461 agcgctcacc gaagcgcctt ctgacgagtt ctcggaggcg tgtttggtca cggctacggc
     2521 gggactgttc ctggcggctt ggcagagtcg tgtgcgcggc tcggagctga gcgagccctc
     2581 accgagcgaa tcccacgcga gggatctcgt gcggctcgga aacggctctg gcgacgaaga
     2641 acgcgcccga gccctcgacg cctacttggt caccgtcgcc gagcacggca tgaacgcatc
     2701 tactttcacg gcgcgcgtgg tcgcttccac cgaatcggac accgtctctg ccgtgacggc
     2761 agcggtcggt gctctcaaag gccggctgca cggcggcgcg cctggcccgg tgctcgacat
     2821 gctcgacgcc gttggcaccg aagatcgagc ccgtgcctat ctcgaagcgg agctcgccgc
     2881 gaatcgacgc atcatgggga tgggccaccg catttatcgc gtgcgggatc cgcgtgctct
     2941 ggtgctcgag cgagcgacgc aacggcttgc cggagcgcaa cataccccgc gtttgaacct
     3001 ggctcggcac gtcgaacgag tcgcgaccga gctcctcgca gagcgacacc cggagcgccg
     3061 cctctgcgcc aacgtggagt ttttcacggc cgtgttgctc gaggccgtga gcgtcccccg
     3121 tgagctcttc acggtgacat tcgccgccgc tcgggttgcg ggctactgcg cgcacatcgc
     3181 agagcagcgg cgccgaggac gtatcgtgcg gccggcctcg cgttacgtcg gcccgcgccc
     3241 gagcggcgag gctcccaccg tgctcgccgt ctcggcctga atcggccgga accggcgaaa
     3301 ccttcgggaa accgcgccct gcggacgcct tccgacgcgt cgcggcgata atgatccagg
     3361 ccctcgcctt tccgagcgaa gcgggtacac tccgcccact tcgcgccggc ccgaccgcgc
     3421 ggagcagaac gcgccccgcg cggcacggag ttaagagcga catgaccgac ttcaagcaaa
     3481 tccaaagcct cctcggcgac aaggcctcct acctgctcga gcaccagagc aagaccgttt
     3541 cgaaggacaa gctccacttg ccttctccga gcttcgtcga cgacgtgtgg gcggcctcgg
     3601 atcgttcgcc gcgcgtgctg cgcgcgcttc agaccctttt caacacgggg cgactcgcga
     3661 acactggcta cctctcgatc ctccccgtgg accagggcat cgagcactcg gccggcgctt
     3721 cgttcgccaa gaaccccgcc tacttcgatc ccgagaacat cgtgaagctc gcgatcgagg
     3781 gcggctgcag cgcggtcgcg tcgacgttcg gcgtgctcgg gatctgcgcc cgcaagtacg
     3841 cgcacaagat cccgttcatg gtgaagctca accacaacga gctgctcagc taccccaaca
     3901 cctacgacca gacgctgttc ggcacggtgc gccagtgctt cgacatgggc gcgcagggcg
     3961 tcggcgcgac ggtgtactgg ggcgcgcccg agtcgcggcg cgagctgcac gaggtgtccg
     4021 aggcgttcgc cgaggcccac cggctcggca tgttcacggt gctgtggtgc tacgtgcgca
     4081 acagcgcgtt caagacggcg cagggcgatc acagcctgtc ggcggatct
//
LOCUS       DASTKS010000037         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_451074, whole genome shotgun sequence.
ACCESSION   DASTKS010000037 DASTKS010000000
VERSION     DASTKS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451074"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFQ35_00785"
     CDS             <1..228
                     /locus_tag="VFQ35_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929183.1"
                     /translation="TPCVSSDPPRKTYAWLAESPKPRAKPTASARAVSSAVRVERRPP
                     DDAGTKAALPDAGATSAPAREDAGAEPETSP"
     gene            225..1154
                     /locus_tag="VFQ35_00790"
     CDS             225..1154
                     /locus_tag="VFQ35_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9929184.1"
                     /translation="MTHAPTLALGVAALLPLLTLFPSPAMGARARLGQSRIVTRSLFD
                     VVSNTLFLRERGSLMVRIEKSTATFGSNEYEVVHAAALCADEPLPQRCTEHTFENELY
                     KRQEQVRAFWIDRYEVTVRDYQRCVSVGRCEAPPYANGAERFNRPDYPVSFVTHADAV
                     AYCRFRNARLPREAEFERAARGVSGRKFPWGELYNAHVANHGRLGVLETDASDGYPEL
                     APVGAFPDGKTPEVIFDLAGNVAEWQAEPYRENSNQEVPAESEAAPRVARGGGFLSSA
                     AFLRGAAREAVPANTRRPDLGLRCARSAQDSAP"
     gene            1151..1699
                     /locus_tag="VFQ35_00795"
     CDS             1151..1699
                     /locus_tag="VFQ35_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9929185.1"
                     /translation="MRDLGSRERVSLPIHANVCFVLVRPHYPENLGASARALKTMGFS
                     DLRVVKPSRLCVPEHEMAFKMAVKAWDVLDAVRTYDDIATAVADCAHVVATTARRGVS
                     GVFTPRTLCPDLLPRAQRGERTAIVFGNEKTGLASDEVELAHAALRIPMAADQPSINL
                     AQAVQLVAYELFVTALEARGVD"
     gene            1705..2727
                     /locus_tag="VFQ35_00800"
     CDS             1705..2727
                     /locus_tag="VFQ35_00800"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET9929186.1"
                     /translation="MRTIDLRSDTVTKPTPAMREAMARAEVGDDVFGEDPTVAGLESR
                     VATLLGKEAALFVPSGTMSNQLALLLHTRPGDEVIVAESAHTAWDESGAGAALAGVQF
                     REVGAQGAFGPDDISRARKPDRYVHPRTRLVCVENTHNRRGGRVFPAELLAAVAARTR
                     ELGLGLHLDGARLWNAACAAALSPLALAEPFDTVSVCFSKGLGAPVGSALCGSSEAIA
                     RARRLRKMLGGGMRQAGVLAAAALFGLEHHRERLGLDHEHARILAEAVASVPGFEVAK
                     TETNIVIARRIDRRATAFVQALASRDVLAIAFDDDTLRLVTHLDVSREDALRAAQEIR
                     ELGRHF"
     gene            2705..>3060
                     /locus_tag="VFQ35_00805"
     CDS             2705..>3060
                     /locus_tag="VFQ35_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929187.1"
                     /translation="MSSGAIFNARVWLTAWVLGACGPSFPPAYVASRTAAEHAYERGD
                     YRHAASLWADAARKAPPGREREEANYRRAVSLERAGDDSAADALYRSLESSPGERSER
                     AAYARAEIAERHVAKT"
BASE COUNT          495 a         1051 c         1033 g          481 t
ORIGIN      
        1 actccgtgcg tcagctcgga tccgcctcga aagacctacg cctggctcgc cgagtcgccg
       61 aaaccgcgtg ccaagccgac cgcgagcgcg cgagccgttt cgagcgccgt tcgcgtcgag
      121 cgccgcccgc cggacgacgc cggcacgaag gctgcgctcc ccgacgcggg agctaccagc
      181 gcgccagcac gcgaagatgc aggggccgaa cccgagacgt cgccgtgacg catgcgccga
      241 cgctcgcgct cggcgttgcg gccctactcc cgctgctgac actgtttccc tcccccgcga
      301 tgggagctcg ggcgcggctc gggcagagcc gaatcgtcac gcgctcgctc ttcgatgtcg
      361 tctcgaacac gttgttcttg cgcgaacgcg gctcgctgat ggtgcgaatc gaaaagagca
      421 cggcgacctt cggctcgaac gaatacgaag tggtgcacgc cgcagcgctc tgcgccgacg
      481 aacctctgcc gcaacgctgc accgagcaca ccttcgagaa cgagctgtac aagcgacagg
      541 aacaggtgcg agcgttctgg atcgatcgct acgaggtgac ggtgcgcgac taccagcgct
      601 gcgtgagcgt aggtcgctgt gaagcgccgc cgtacgcgaa cggcgccgag cgcttcaatc
      661 ggcccgatta cccggtgagc ttcgtcactc acgccgatgc ggtcgcgtat tgccgctttc
      721 ggaacgcgcg cctcccccgc gaagcggagt tcgagcgcgc cgcgcgcggc gtctccggcc
      781 gcaaattccc gtggggcgag ctctacaacg cgcacgtcgc aaaccacggc cgcctcggcg
      841 tgctcgaaac cgacgcgagc gacggctatc ccgagcttgc gccggtcggt gcctttcccg
      901 acggtaagac gccggaagtg atcttcgatt tggcgggtaa cgtcgccgag tggcaggcag
      961 agccgtaccg cgagaattcg aaccaagagg tgcccgccga gtccgaggcg gcgccgcgcg
     1021 tcgcgcgcgg gggtggcttt ttgagcagcg ccgcgttctt gcgtggagcg gctcgcgaag
     1081 cggtaccggc gaacacgcgc cgtcccgacc tcggcttgcg ctgcgcgaga agcgcgcaag
     1141 actcggcgcc gtgagagatc tcgggagccg cgagcgcgtt tcgttaccga ttcacgcgaa
     1201 cgtgtgtttc gtcctggtgc ggccgcacta cccggagaat ctgggagcgt cggcgcgggc
     1261 cttgaagacg atgggatttt cggacctgcg cgtagtcaag ccgagccggc tgtgcgtgcc
     1321 ggagcacgaa atggcattca agatggccgt caaggcgtgg gacgtgctcg atgccgttcg
     1381 cacctacgac gacatcgcga cggccgtcgc cgactgcgcc cacgtcgttg caacgacggc
     1441 tcgccgcggt gtcagcggag tcttcacgcc gcgcaccctc tgccccgatt tgcttccgag
     1501 agcccagcgc ggcgagcgca ccgcgatcgt tttcggcaac gagaagaccg ggctcgcgag
     1561 cgatgaagtc gagctcgccc acgccgcgtt gcgcatcccg atggcagcgg accagccctc
     1621 gatcaacctc gctcaggcgg tgcagctcgt agcgtacgaa cttttcgtca ccgcgctcga
     1681 ggctcgcggc gtagactgac cttcgtgcgc acgatcgatc tgcgttcgga caccgtaacg
     1741 aagccgacgc ctgccatgcg cgaggcgatg gcccgcgccg aagtcggtga cgacgtcttc
     1801 ggggaagatc cgacggtcgc agggctcgaa tcgcgcgtcg cgacgctgct cggcaaggaa
     1861 gcggctctct tcgtgccctc cgggaccatg agcaatcagc ttgcgctgct cttgcacacg
     1921 cgaccgggcg acgaagtcat cgtggcggag tctgcccaca ccgcttggga cgagtcgggc
     1981 gcgggcgctg cgctcgccgg agttcagttc cgagaagtcg gcgcgcaagg cgctttcggt
     2041 cccgacgaca tcagccgcgc tcgcaagccg gaccgctacg ttcacccccg cacccgcctc
     2101 gtttgcgtgg agaacaccca caaccgcaga ggcgggcgag tcttcccggc agagctcttg
     2161 gccgcggtgg cagcgcgaac gcgggagctc gggctcggtt tgcatctcga tggcgcgcgc
     2221 ctgtggaacg ccgcgtgcgc cgcggcgctg tcccccctcg cactcgccga accgttcgac
     2281 accgtgagcg tctgcttttc caagggcctg ggcgcgcccg tcggttctgc gctttgcggc
     2341 tcgagcgaag ccatcgcccg agcgcgacgc ttacgtaaaa tgctcggagg aggcatgcgg
     2401 caagcgggtg tgctcgcggc cgcggcgctg ttcggcctcg agcatcaccg cgaacggctc
     2461 ggcttggacc acgagcacgc tcgcattctc gcggaagcgg tcgcgagcgt gcctggcttc
     2521 gaggttgcca aaaccgaaac caacatcgtc atcgcgcgcc gaatcgatcg acgggcgacg
     2581 gcgttcgtgc aggcgctggc ttctcgcgac gtgctcgcga tagccttcga tgacgacacg
     2641 ctccgattgg tcacccacct cgacgtgagc cgcgaagacg cgctccgcgc cgcgcaggag
     2701 atccgtgagc tcgggcgcca tttttaacgc gcgcgtttgg ctcacggcct gggtgcttgg
     2761 agcctgcggt ccgagctttc ctcccgcgta cgtcgcaagc cgaactgcag ccgagcacgc
     2821 ctacgagcgc ggcgattacc gccacgccgc gtcactgtgg gcggacgccg cgcgcaaggc
     2881 tccgcctggg cgcgaacgag aggaggcgaa ttaccggcgc gcggtgagcc tggaacgcgc
     2941 cggtgacgat agcgccgccg acgctcttta tcggtcgctc gagtcttcgc ctggagagcg
     3001 ctcggagcga gccgcatacg cgcgggccga gatcgccgag cgccacgtgg cgaaaaccga
//
LOCUS       DASTKS010000038         5117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_896631, whole genome shotgun sequence.
ACCESSION   DASTKS010000038 DASTKS010000000
VERSION     DASTKS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896631"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VFQ35_00810"
     CDS             <1..1330
                     /locus_tag="VFQ35_00810"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET9929188.1"
                     /translation="DAPSIEKVVVYRRLGQERLPIEMKAGRDVEWKELVDGARDAAPS
                     ASAPEIVDAEHPLFILYTSGSTGKPKGVLHTSAGYLAGTHITSKYVFDLRDDDVYWCT
                     ADVGWVTGHSYIVYGPLSNGATCVLYEGAPNAPDWGRFWRIVEKHKVTILYTAPTAIR
                     AFIRQGDEWPRKADLSSLRLLGSVGEPINPEAWVWYYEHIGQKRCPVVDTWWQTETGS
                     ILLTTLPGAHRMRPGATGLPFFGVEPQVVDAQGTRVPAGSGGFLVLRRPWPSMLRTVW
                     GDDERFRRQYFSDIEGCYFTGDGAREDEHGYFHVVGRIDDVLNVSGHRIGTAEIESAL
                     VSHPAVAEAAAVGRPDDLKGQALVVFVTLKPPNEPSEEMKQKLMAHVAEEIGKFARPD
                     VVRFAESLPKTRSGKIMRRLLKDVAAGRDTQGDVSTLEDLNVLSKLRAEE"
     gene            1401..2183
                     /locus_tag="VFQ35_00815"
     CDS             1401..2183
                     /locus_tag="VFQ35_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET9929189.1"
                     /translation="MKAIVIGAGRGSRLGPLTEEIPKTLVPVLGRPMLDQILDALAAA
                     GFTRDRVVFVCGYRAEVVRQRYPELTFVHNAGWESNNILCSLLCAREHLGEGFVSTYA
                     DIVYEPGIVQKLVAAPEDLVLGCDTQYRRRYAGRTQHPESDAEKMTAAGRRITRLSRH
                     IPSEEACGEFIGVMKASPVGARSFMHAFDEAQRRFAGRTFREGRSFERAYLIDLLQDM
                     LEQGVAMHREDTPGGYMEIDTEQDVAFANEWWNAWQATASNP"
     gene            2144..2977
                     /locus_tag="VFQ35_00820"
     CDS             2144..2977
                     /locus_tag="VFQ35_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET9929190.1"
                     /translation="MVERVASDGIEPLSIDVAALLEKLSGACAREPKPVFAFDGDGTL
                     WSGDVGEDVFRRATRLGLLREESRAALLREAQRYAVETPDPSDANAVALRLFESYLSG
                     GYPEREACAMMTWCYAGLELEEFTPFVRATFDEVGLRGRLHQELGPVLELARSHHAEL
                     LVVSASPQRIVEEAAALWGIPAQSVLASQPAVRDGRIQAQLARPVPYAEHKPLTLAER
                     ANLAHLVAAFGDNVFDIELLTAARVGVAVRPKAALRTRLRELTGVYVLRAPVESGSHS
                     Y"
     gene            2994..3713
                     /locus_tag="VFQ35_00825"
     CDS             2994..3713
                     /locus_tag="VFQ35_00825"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9929191.1"
                     /translation="MITRSSLFVVLGAWLCVSCGGSEPPPETPDHAAHAPRQHRRGNG
                     AVGVASEIGGMNEERVDATFKRSLDALQECLNKGSERVEYLGGSVAFFVRVNSEGKAE
                     HVHLERSTLGDRQTEKCMLSTLRARRWPKPVGGMNGLARKSFEFDPPSDVRPPIDWDP
                     ENVKKGLDKISDKLTECKEGDSGKFEATMYVSAEGSVLAASVTPPDEAGESSVDCLVD
                     ALRSASYPSPGSWVAKVSFSL"
     gene            3710..4462
                     /locus_tag="VFQ35_00830"
     CDS             3710..4462
                     /locus_tag="VFQ35_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9929192.1"
                     /translation="MTPRLQGQRALVTGAATRLGRVIARALAAQGVDLVLHYHSNRAG
                     AEQARDELAALGVNVELASADLTDRRQARELVASAAATLGGLDLLVASAANFERVELA
                     AVDDEAWDRSLDLNLSSQFSLAHAALPHLRTSRGSIVFITCSSATTPFRNYLPYVVAK
                     GGLRHLMRTLALEAAPEVRVNAVAPGTVLPAEGTPPELLDRIKRRIPLARLGAPEDVA
                     EAVVFLASASFITGHEIVVDGGRSLAAVETFG"
     gene            4555..>5117
                     /locus_tag="VFQ35_00835"
     CDS             4555..>5117
                     /locus_tag="VFQ35_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9929193.1"
                     /translation="MEWLNYHHLLYFWTVAREGGLVPAGKVLHVSHPTLSAQIHKLEE
                     TLGEKLFLKQGRRLVLTDTGRTVYRYAEEIFSLGREMMDTVKGRATGKPMRLNVGVAE
                     TLSKLVVRRLIEPALAVPESIKLVCYEDRYPRLLADLAMHSLDIVLTDAPLPPGSSIK
                     AFNHFLGECGIAFFGTPSLARAHQRNFP"
BASE COUNT          900 a         1681 c         1671 g          865 t
ORIGIN      
        1 ggacgcgccc agcatcgaga aggtcgtggt gtaccggcgg ctcgggcaag agcgcctccc
       61 gattgaaatg aaagcgggtc gggacgtgga gtggaaggag ctggtcgacg gcgcgcgcga
      121 cgcggctccc agcgcttccg ccccggagat cgtggacgcc gagcacccgc tcttcattct
      181 ttatacgtcc ggttcgacgg gcaagcccaa gggcgtgttg catacgagcg cgggctacct
      241 ggcgggcacg cacatcacat ccaagtacgt gttcgacctg cgggatgacg acgtgtactg
      301 gtgcacggcc gacgtgggtt gggtcaccgg ccacagctat atcgtgtacg gaccactctc
      361 gaacggcgcc acctgcgtgt tgtacgaagg tgcgccgaat gccccagatt gggggcgctt
      421 ctggcgcatc gtcgagaaac acaaagtcac cattctgtat acggccccca cggccattcg
      481 ggccttcatc cgccaaggcg acgagtggcc gcgcaaggcc gacctctcga gtctgcgact
      541 gctcggcagc gtgggtgagc cgatcaaccc cgaggcatgg gtctggtact acgagcacat
      601 cggacaaaag cgctgccccg tggtcgacac ctggtggcag accgagaccg gctcgatcct
      661 gctcaccacg ttgccaggcg cgcatcgcat gcgtccgggc gcgacggggc tgcccttctt
      721 tggagtggag ccgcaggtcg ttgacgctca aggcacgcgc gtgcccgcgg gttccggcgg
      781 cttcctagtg ctgcgtcggc cgtggccgag catgctgcgc accgtgtggg gagatgacga
      841 gcgcttccgt cgccagtact tcagtgacat cgagggctgc tacttcactg gcgatggcgc
      901 tcgcgaagac gagcacggct acttccatgt ggtgggccgc atcgacgacg tgctgaacgt
      961 ctcgggtcac cgcatcggca ccgccgaaat cgagagcgcg ctggtctcac acccggccgt
     1021 cgccgaggcc gctgcggtgg ggcgtccgga cgatctcaag ggtcaggcct tggtggtatt
     1081 cgtcaccctg aagccaccca acgagccgag cgaagagatg aaacagaagc tcatggcgca
     1141 cgtagccgaa gaaatcggca agtttgcgcg cccagacgtc gtgcgcttcg ccgaaagcct
     1201 gcccaagacg cgcagcggca agatcatgcg gcgtctactc aaggacgtcg ccgcgggccg
     1261 cgacacgcaa ggggatgtgt caacgctcga agatttgaac gtgctttcga agctgcgtgc
     1321 ggaagaatag ggtcccttgg gtcgggcccg cgccggccgc cagtgaaacc gcgcgctggc
     1381 tcgaccaact agaccctccc atgaaagcca tcgtgatcgg cgcgggtcga ggtagccgcc
     1441 tcggcccgct cactgaagaa atccccaaaa cattggtgcc ggtgctcggc cgcccgatgc
     1501 tcgatcagat cctggacgcg ctcgccgccg ccggcttcac gcgagaccgc gtcgtcttcg
     1561 tgtgtggtta ccgagccgag gtcgttcgcc agcgctaccc cgagttgacg ttcgtgcaca
     1621 acgccggctg ggaatccaac aacatccttt gctcgctgct gtgcgcgcgc gaacaccttg
     1681 gtgaggggtt cgtctcgacc tacgcggata tcgtgtacga gcccggcatc gtgcagaagt
     1741 tggtggccgc acctgaagac ctcgtgctcg gctgtgacac gcaatatcgt cggcgctacg
     1801 cgggtcgcac ccagcacccc gagagcgacg ccgagaagat gacggcagcg gggcgccgca
     1861 tcacgcggct gtctcgtcac attccgagcg aggaagcttg cggggagttc atcggcgtca
     1921 tgaaagcgag ccccgtcgga gctcgctcct tcatgcacgc cttcgacgag gcgcagcgcc
     1981 gtttcgccgg tcgcaccttt cgcgaaggcc gcagcttcga gcgcgcctac ttgatcgact
     2041 tgctgcaaga catgctggag cagggtgtcg cgatgcatcg cgaggacact cccggcggct
     2101 acatggagat cgacaccgag caagacgtgg ccttcgcgaa cgaatggtgg aacgcgtggc
     2161 aagcgacggc atcgaacccc tgagcatcga cgtagcggcg ctgctcgaaa agctgagcgg
     2221 cgcgtgcgcg cgcgagccga aaccggtatt cgctttcgac ggcgacggca cgctctggtc
     2281 gggtgatgtc ggagaagacg tattccgccg cgcgactcgg ctcggcttgt tgcgcgaaga
     2341 gagccgcgca gctctgctac gggaagccca gcgttacgcc gtggagacgc ccgatccgag
     2401 cgacgccaac gccgttgccc tgcggctctt cgaatcgtat ctcagcggtg gctatcccga
     2461 gcgcgaggcc tgcgccatga tgacgtggtg ctacgcaggc ctcgagctcg aagagttcac
     2521 cccattcgtg cgagccactt ttgacgaagt cgggctgcgc gggcggctcc accaggagct
     2581 cggacccgtg ttggagctcg ctcgttcgca ccacgcggag ctgttggtgg tctcggcatc
     2641 gccgcaacgc atcgtcgagg aagcggcggc attatgggga atacccgctc aaagcgtgct
     2701 cgccagccag ccagccgtgc gcgacggtcg catccaagcg caactggccc gtccggtgcc
     2761 gtacgccgaa cacaaacccc tgacgctcgc ggagcgagcg aatctggcgc acttggtggc
     2821 ggccttcggg gacaacgtct tcgacatcga gctcctgact gcggcgcgcg ttggagtcgc
     2881 cgtgcgcccc aaagccgcct tgcgaacgcg cttgcgcgag ctgaccggtg tttacgtgtt
     2941 gcgcgcccct gttgagagcg gaagtcattc atattaaaga gcgaactctc gtcatgatca
     3001 ctagatcgtc gttgttcgtg gtgttgggtg catggctttg cgtgagctgc ggcggtagcg
     3061 agccgccgcc ggaaaccccg gatcacgccg cccacgcccc gcgccagcac agacgcggca
     3121 acggcgcggt gggcgtggcg agtgagattg gcggcatgaa cgaagagcgc gtggacgcga
     3181 ccttcaagcg ctcgctcgat gccctccaag agtgtttgaa caaaggctcc gagcgcgtcg
     3241 aatatctcgg cggttcggtg gcgttcttcg tgcgcgtcaa ctcggaaggc aaggccgagc
     3301 acgtgcacct cgaacgctcg acgctcggcg atcgtcagac cgagaagtgc atgctgtcga
     3361 ctttgcgcgc acggcgctgg cccaagccgg tcggcggcat gaacggcctc gcccgcaagt
     3421 ccttcgagtt cgatccgccg agcgacgtgc gcccgccgat cgactgggac ccggaaaacg
     3481 tcaagaaggg cctcgacaag atcagcgaca agctgaccga gtgcaaagag ggagattccg
     3541 gcaagttcga agccacgatg tacgtgtccg ccgagggctc ggtgctggcg gccagcgtca
     3601 ctccgccgga tgaagcgggc gagagctccg tcgattgcct cgtggacgcg ctgcggtctg
     3661 ccagctatcc gtctccgggc agttgggtcg ccaaggtatc tttctcgcta tgacgccccg
     3721 cctgcagggc cagcgagcac tcgtcacggg agccgccacc cggctcgggc gcgtgatcgc
     3781 gcgcgcgttg gcggcgcagg gggtggacct ggtgctccac tatcacagca accgcgcggg
     3841 agccgagcaa gcgcgtgacg agctcgcggc gctcggagtg aacgtggagc tcgcgagcgc
     3901 agacttgacc gatcgtcgac aagcgcgcga gctcgtcgcg agcgccgctg caacgctcgg
     3961 aggcctcgac cttttggtcg cgagcgcggc caatttcgag cgcgtggagc tcgcggccgt
     4021 cgacgacgag gcgtgggacc gttccttgga tttgaatctc tcgtcccaat ttagcctggc
     4081 ccacgcggca ttaccgcatc tgcggaccag ccgcggctcc atcgtgttca tcacctgctc
     4141 gagcgccacc acgccgttcc gaaactatct cccctacgtc gtggccaagg gggggttgcg
     4201 gcacctgatg cgcacgcttg cgctcgaggc cgcgccagaa gtgcgcgtga acgccgttgc
     4261 gccgggcacc gtactccccg ccgaaggaac gcctccggag ctactcgatc gcatcaagcg
     4321 gcgcatcccg ctggctcgcc tcggcgcgcc ggaagacgtg gcggaagcgg tcgtcttctt
     4381 ggcgagcgcg tcgttcatta cggggcatga aatcgtggtg gatggcgggc gcagcctggc
     4441 tgccgtcgag accttcggct aagctctaga cagcgccaac tccacgcagc tcgccgctcc
     4501 tcgggcaaac cgagccgtgc aacacgccag cccgggcgac atactacgcg cgccatggag
     4561 tggctcaact accaccacct gctctacttc tggaccgtgg cccgcgaggg cggcctcgtt
     4621 cccgcgggca aggtgctgca cgtctctcat cccacgctga gcgcgcagat ccacaaactc
     4681 gaagagacgc tcggggagaa gctgttcctc aagcaaggtc gtaggctcgt cctgaccgac
     4741 acgggacgca ccgtgtatcg ctacgccgaa gagatcttct cgctcggccg cgagatgatg
     4801 gacaccgtca aaggccgcgc cactggcaag cccatgcgct tgaacgtcgg cgtggccgag
     4861 accttgtcca agctggtcgt ccgtcgcttg atcgagccgg cgctggccgt cccggagtcc
     4921 atcaagctcg tctgctacga ggatcgctat ccgcgcttgc tcgccgatct agccatgcac
     4981 tcgctcgaca tcgtcctcac cgacgccccc ctcccccccg gcagcagcat caaagccttc
     5041 aatcacttcc tgggcgagtg cggcatcgcc ttcttcggaa ccccgagcct ggcgcgcgct
     5101 catcaacgca atttccc
//
LOCUS       DASTKS010000039         6755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_896786, whole genome shotgun sequence.
ACCESSION   DASTKS010000039 DASTKS010000000
VERSION     DASTKS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896786"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /gene="tyrS"
                     /locus_tag="VFQ35_00840"
     CDS             <1..1043
                     /gene="tyrS"
                     /locus_tag="VFQ35_00840"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9929194.1"
                     /translation="PSGKSAERQMMTLEQVEQNVAAIRPIYERLLDFSGSNAARLRNN
                     ADWLKGIGYLEMLRDVGKYFSVNMMIQKDSVRERLQNREQGISYTEFSYMLLQAYDFM
                     YLFEHEQVTVQVAGSDQWGNTVAGVDLIRRVHRTETFGLTTPLVTKSDGGKFGKTESG
                     AIWLTADRTSPYEFYQFWLNVADADIPVYLNTFTFFEQPRISELIASQTENPGARAGQ
                     RALAAHMTELLHGKEGLAQAETATQALFSGDVGGLDKSTLESVFRNVPTGTLTKSSLE
                     GEGIPLIDALVETGVAKSKRDARELLSKNAILVNGRKASLDDRLSLEWLLHGEMLLVR
                     RGKKEWHVVRVR"
     gene            1136..2380
                     /locus_tag="VFQ35_00845"
     CDS             1136..2380
                     /locus_tag="VFQ35_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9929195.1"
                     /translation="MRRSRRLLPLLALLSWAAPSVAAERRVPFREAVGAGDQNPRVVS
                     TRQASESLSRAEQKSPSLDANPVVTGAVGPRVAPASERGVDWGVGLSQSFSVHGAAAR
                     RREGLAAEAEWLRADADAELVARRIAAASAWLTLWEAERQVALTQEDVTNEERLLALV
                     KRLAEVQERTAADVATVETRLAESKLRERLAEGTLVEAKARLSAEMHAARDDDLVTDG
                     ALPELPMPERAEALQLIARAASLPAVRARQLLSRAEQARAREERAGREARLSFGIDFR
                     HESPGANIVQANAIVPLPIFAVGAREYASRMATSLRASGEAADEAMRAREAMRLAVHE
                     LEHSQEVFELLDARLVPSAERAIQLRERQLKGGEGTLLEVIDARRTFLDARLRWLSAA
                     KNRAWARIQVELLARAAAGNGK"
     gene            2377..3432
                     /locus_tag="VFQ35_00850"
     CDS             2377..3432
                     /locus_tag="VFQ35_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929196.1"
                     /translation="MKALPFLLLLPLVVACKQSDAPTTTGEAPRDVQPSSSFKWVPVR
                     PSSDAPPEEYPARLLSSAGTVAVSVAPLPARIISLAVKPGDDVEKGGAIATVVMPEAD
                     AAIATLRAAETSLGVLTKRRAQLGGLESEGLVKASDLAALDLDIARLKGEKVRAEAVL
                     QGAGLKAGGTVTLRSPVAGVVTEVAAIQGELRRPEDGPIARIRSRTGQRIEAMFPERP
                     PTDATFSFRLGTEPLVPVKLVNVVPQSPGVGFVAWFDTQGDISLGAGTEGRIAVSASA
                     SADAYLVPSSAVGSRGAQRFVVARTRTDVKSAELPVQVLRVTSSDSIVRGALSESSLV
                     ATEPYRAGSELAGEKRP"
     gene            3429..6551
                     /locus_tag="VFQ35_00855"
     CDS             3429..6551
                     /locus_tag="VFQ35_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET9929197.1"
                     /translation="MIEAMIRFAVTKRTWVVVIWLGAALGLLALAVRLKLDALPDITS
                     NQVQVLTRAPGLTPDEVELRVTRPLEATLGGLAGLSNSRSVSRFGLSAITLVFDDDVD
                     ILRARQLAAERITLVGGRLPHGVEAPELGPITGGLGEVFHFTVSAPTRTPYELLELVE
                     LRVAPIVKTVPGIVELNTWGGAERTLEVRVDANRMLLRHVSFEELRQALTRTIGSQPG
                     ASLDAGARHVLLRGSFLPTRARELADALVKMDGPNAIRVSDVATVGEGIAPRLGAATR
                     DGRGETVYVMAQMLIGANARDVTHAVRAKMSQVRAALPDDVRIQTVYDRSELVDATLR
                     TVAKSLVEGGLLVCVVLFLMLGSARAGIVVALTIPVAMLGATAALSLLGVSGNLMSLG
                     AVDFGLLVDGAVVLIEHVFRHDDDHRDLGWAERVTRACAAVARPSFFGVSVILLVYVP
                     VLSLTGVDGKMFRPMALTVVLALLVALLFTLTFIPAAAALFLRDRDIPSRPPLMQRTV
                     AAFHSAVLRRVVPRPGLVLALSLFALGASIHVMTQLGSELAPTLDEGSLVIQTTRSAD
                     LSVEGAIAEASKMELALKSGFPEVKAIVSRVGSPAVATDTMGLEQADVFVELAKPGEW
                     RAGMTRSKLLSELERRIETTTPGSEPAFTQPIQMRFNELLGGAPYDVVVSLIGQDATA
                     LRQSAREVVALVAKISGVQDPRMLAQDELPVQEVRPRPLAAAQRGFAVGDVLDLVGAM
                     RLGVPVGTTYDGPVQIPVTLRLGTAAPGALVLGDTLLPGPKSELVPLASVADVRQLSA
                     PAALYRWNGERRALVGFNVRDRELGEVVAEASTKVKSDIKLTEGTRIEWGGQYETLRA
                     AQQRLMLVIPAVLLLIVFVLFLHFGEAGPVAWVLSHVPFAAVGGTLALLARGMPLSIS
                     AGIGFIALWGIAVMNGTVLVTEILELERRGFAPREATLKAAEDRARPVSMTAMVAALG
                     FLPMAIAQGPGAEVQRPLATVVIGGLVSSTTLTLLVLPTLRAAFAQRFRRRRDAKVAA
                     APGVPEEIQS"
     gene            complement(6563..>6755)
                     /locus_tag="VFQ35_00860"
     CDS             complement(6563..>6755)
                     /locus_tag="VFQ35_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929198.1"
                     /translation="HAQKPDDLYLPPPTGFDIGLNIRPIPDVVVKVDFSLATWGKPDR
                     VGLSRYPLKQLGTQVAWAF"
BASE COUNT         1100 a         2265 c         2296 g         1094 t
ORIGIN      
        1 acccctcagg caagagcgcc gaacgccaga tgatgacgct cgaacaagtc gagcagaacg
       61 tggccgcgat tcgcccgatt tacgagcgct tgctggactt ttccggcagc aacgccgcgc
      121 ggctgcgcaa caacgccgat tggttgaaag gcatcggcta cctcgaaatg cttcgagacg
      181 tcggtaagta cttctcggtc aacatgatga tccagaagga ctcggtgcgt gagcggctgc
      241 agaaccgcga acagggcatc agctacaccg aattcagtta catgctgctg caggcctacg
      301 acttcatgta cctgttcgag cacgagcagg tcacggtgca ggtggcaggc tcggaccagt
      361 ggggcaacac cgtcgccggc gtcgatctga ttcgtcgcgt gcaccgtacc gagacgttcg
      421 gcctcacgac cccgctcgtg accaagtcgg acggcggtaa gttcggtaaa accgagagcg
      481 gcgccatttg gctgaccgcg gaccgcacgt caccgtacga attttaccaa ttctggctga
      541 acgtggccga cgcagacatc cccgtctacc tgaacacctt cacgttcttc gaacagccgc
      601 gcatctcgga gctcatcgcg tcacaaaccg agaacccggg ggcgcgcgcc ggtcagcgcg
      661 ctctcgccgc gcacatgacc gagctcttgc acggcaagga ggggctcgcg caggccgaaa
      721 cggcgacgca agcgttgttc tccggtgacg tcggcggtct cgacaagagc acgctagaga
      781 gcgtctttcg gaacgttccg accggaacgc tcacgaagag cagcctcgaa ggggaaggca
      841 tcccgctcat cgacgcgctg gtcgagaccg gcgtcgcaaa gagcaagcgc gacgcgcgcg
      901 agctgctctc gaagaacgcg atcctggtga acgggcgtaa agcgtcgctc gatgaccgct
      961 tgagcctcga gtggctgctg cacggtgaaa tgcttctcgt ccgccgcggc aagaaggagt
     1021 ggcacgtcgt acgcgtgcgc tgaaaaccct gacgaaacgc gcctccgtcg gggtctggcg
     1081 cgcggcgtgg cacggatcat gtcccgcgcg gcgaagatgc actatctatt gacccatgcg
     1141 tcgttcgcgc cgcctcctac ccctcctcgc tctgctgagc tgggcggcgc catccgtcgc
     1201 ggccgagcgc cgggtgccgt ttcgggaggc tgtcggcgcc ggcgaccaga acccgcgcgt
     1261 ggtgagcact cggcaggcga gcgagagcct gtcacgagca gagcaaaagt ctcctagcct
     1321 cgacgcaaac ccggtcgtca cgggagccgt aggtccgcga gtggcgcccg cgtccgagcg
     1381 cggcgtcgac tggggcgtgg gcttgagcca gtcgttcagc gtgcacggcg ctgcagcccg
     1441 tcggcgcgaa gggctcgccg ccgaagccga gtggttgcgc gccgatgcag acgcggagct
     1501 cgtggcgcgg cgcatcgcag cggcttcggc gtggctcacg ttgtgggaag cggagcggca
     1561 ggtcgcgctc actcaagaag acgtgaccaa cgaggagcgc ttgctcgcgc tcgtgaaacg
     1621 cctcgcagaa gtgcaggagc gaaccgccgc cgacgtcgcg accgtcgaga cacggctcgc
     1681 cgagtcgaag ctccgcgaac ggctggcaga aggcactttg gtcgaggcca aggcgcggct
     1741 ctcggcggag atgcacgcgg cgcgcgacga cgatctcgtg accgacggcg ccctgccaga
     1801 gctcccgatg ccggaacgag ccgaggcgtt gcagctcatc gcgcgcgcgg cgtcgctgcc
     1861 ggccgttcgc gcgcggcagc tgctctcccg agccgagcaa gcgcgagcac gagaagagcg
     1921 cgctggacga gaggcgcgct tgagtttcgg gatcgacttt cgccacgagt ctcctggcgc
     1981 caacatcgtg caagcgaacg cgatcgtgcc tttgccgatt tttgcggtgg gcgctcgaga
     2041 gtacgcctcg cgcatggcca cgtcactgcg agcgagcggc gaagccgcgg acgaggcaat
     2101 gcgcgcacgt gaggcgatgc gcctcgccgt gcacgagctc gagcacagcc aagaggtgtt
     2161 cgagcttctc gacgcgcggc tcgtcccgag cgccgagagg gccattcaac tgcgcgaacg
     2221 acagctcaag ggcggcgaag gcaccttgct cgaggtcatc gacgctcgcc gcacgttcct
     2281 ggacgcgcgc ctgcgctggc tgagcgcggc gaaaaaccgc gcctgggctc gcatccaagt
     2341 cgaattgctg gcgcgagccg cggcggggaa cggtaaatga aggctctccc cttccttctc
     2401 ctgctacccc tggtcgtcgc ctgcaagcag agcgacgcgc ccacgaccac cggcgaagca
     2461 ccgcgcgacg tccagccgag ctcctccttc aaatgggtgc cggtgcgccc ctcgagcgac
     2521 gctccccccg aggagtatcc agcgcggctc ttgagctccg cgggtacggt tgcggtcagc
     2581 gtcgcaccgc tgcccgcgcg catcatctcg ctggcggtga agcccggtga cgacgtcgag
     2641 aagggcggcg cgattgcgac cgtcgtcatg ccggaggccg acgctgcgat cgcgacgctc
     2701 cgcgccgcgg agacgtcact cggcgtcctc accaagcggc gcgcacagct cggtgggctc
     2761 gagtccgagg gcctggtgaa ggcgtcggac ctcgccgcgc tcgacttgga catcgcccgc
     2821 ttgaaaggcg aaaaggtgcg agcggaagcc gtgttgcagg gcgcgggcct caaggcgggc
     2881 ggcaccgtca cgctgcggag ccccgtagcc ggcgtcgtca ccgaagttgc ggctattcaa
     2941 ggcgagctac gtcgccccga ggacggaccc atcgcgcgca ttcgcagccg cactgggcag
     3001 cggatcgaag ccatgtttcc cgagcgccca cccaccgacg caaccttctc gttccggctc
     3061 gggacggagc cgctggttcc cgtgaagctc gtgaacgtcg tgccgcagtc gccaggcgtc
     3121 ggcttcgtcg cttggttcga cacgcagggc gacatctcgc tcggcgcggg caccgagggt
     3181 cgcatcgccg tgagcgcctc cgcgagcgcg gatgcttact tggtcccgtc gagcgccgtc
     3241 ggctcgcgcg gcgcccagcg cttcgtagtc gcgcgaacac gcaccgacgt gaaatccgcc
     3301 gagctgccgg tgcaagtgct tcgcgtcacg tcgagcgaca gcatcgtgcg gggcgctctg
     3361 agtgaaagct ctttggtcgc gaccgagccg tatcgcgccg gtagcgagct cgccggagag
     3421 aagcggccgt gatcgaggca atgattcgct tcgcggtgac gaagcgaacc tgggtcgtgg
     3481 tcatctggct cggtgcggcg ctcgggctcc tcgctctcgc ggtgaggctc aagctcgacg
     3541 ccttgcccga catcacgagc aatcaagtgc aggtgctcac gcgcgcgccg gggctcaccc
     3601 cggacgaagt cgagctccgg gtcacgcgtc ccttggaggc gaccctcgga ggcctcgctg
     3661 gcctctcgaa cagccgcagc gtctcgcgct tcgggttgtc ggcgatcacg ctcgtcttcg
     3721 acgacgacgt ggatattctg cgagctcgcc agctcgcagc cgagcgcatc accttggtgg
     3781 gcggacgctt gccacatggc gtcgaagcgc cggagctcgg gcccattacg ggcggcctcg
     3841 gcgaggtctt tcacttcacc gtttccgcgc ccacccgcac gccgtacgaa ctcttggagc
     3901 tcgtcgagct ccgagtcgcg ccgatcgtaa agacagtgcc gggcatcgtc gagctcaaca
     3961 cgtggggcgg cgccgagcgc acgctcgaag tgcgcgtcga cgcgaatcgc atgctactgc
     4021 gtcacgtttc gttcgaagag ctgcgacaag cgctcactcg aaccatcggc agccaacccg
     4081 gcgcgtcgct cgacgcaggc gcccgccacg tgctgctgcg cggctcgttc ttgcccactc
     4141 gagcgcgcga gctcgccgac gccctcgtaa agatggacgg cccaaacgcg attcgcgtga
     4201 gcgacgtggc caccgtcggc gaaggcatcg cgccgcggct cggcgcggcc acgcgcgacg
     4261 gtcgcggcga gaccgtctac gtcatggccc aaatgctgat cggagcgaac gcgcgtgacg
     4321 taacgcatgc ggtgcgcgcg aagatgagtc aagtgcgcgc cgcccttccc gacgacgtcc
     4381 gcatccagac cgtctatgat cgcagcgagc tcgtcgacgc gacactccgc accgtcgcca
     4441 agagcctcgt cgagggcggt ctcctggtgt gcgtcgttct cttcctgatg ttggggagcg
     4501 cccgcgccgg catcgtggtc gcgctcacga tccccgtggc aatgttgggg gcgacagcgg
     4561 ccctctccct acttggggtc tcgggaaatc tgatgagcct cggcgcggtc gacttcgggc
     4621 tcttggtcga cggcgcggtc gtgctcatcg agcacgtgtt ccgccatgac gacgaccata
     4681 gagatctcgg gtgggccgag cgcgtgacga gagcttgcgc ggcggtggcg cgcccctcgt
     4741 tcttcggcgt ctccgtcatt ctgctcgtct acgtgccggt gctctcgctc accggcgtcg
     4801 acgggaagat gtttcggccg atggcgctca cggtggtgtt ggcgctcctg gtcgcgctgc
     4861 tcttcacgct gaccttcatc cccgccgcgg cggcgctctt cttgcgcgac cgcgacatcc
     4921 cttcgcggcc accgctgatg cagcggaccg tcgccgcgtt ccactccgcc gttctgcgtc
     4981 gcgtcgtgcc gcgaccgggg ctcgtgctcg cgttgtcgct cttcgcgctc ggcgcctcca
     5041 ttcacgtgat gacgcagctc ggcagcgagc tcgcgccgac gctcgacgaa gggtcgctcg
     5101 tgattcaaac gacccgaagc gcggatctca gcgtcgaagg agccatcgcc gaagcaagca
     5161 agatggagct tgcgctgaag agcggcttcc cggaagtgaa agcgatcgtg agccgcgtcg
     5221 ggagccccgc cgtcgccacc gacacgatgg ggctcgaaca agccgacgtg ttcgtcgagc
     5281 tcgccaagcc cggcgaatgg cgtgccggca tgacccgcag caagctgctc tccgagctcg
     5341 agcggcgcat cgagaccacc actcccggct cggagccggc gttcactcag cccatccaga
     5401 tgcgcttcaa cgagttgctc ggcggcgcgc cgtacgacgt cgtcgtgagc ttgatcgggc
     5461 aggacgcgac tgctctacgg cagagtgcgc gcgaggtcgt cgccttggtc gcgaagattt
     5521 ccggcgttca agatccgcgc atgctcgcgc aagacgagct ccccgtgcag gaggtgcgac
     5581 cgcggccact cgcggcggcg caacgcggct ttgcggtcgg agacgtgctg gatttggttg
     5641 gcgcgatgcg gctcggcgtg ccggtcggta ccacgtacga cgggcccgtc caaatcccgg
     5701 tgacgctccg cctcggcacg gccgcaccgg gtgcgctggt gctcggagat acgctgctgc
     5761 ctgggccgaa gagcgagctc gtgccgctcg cgagcgtggc cgacgtgcgg cagctctcgg
     5821 cgccggccgc cctctaccgc tggaacggag agcggcgcgc gctcgtgggc ttcaacgttc
     5881 gcgaccgcga actcggggaa gtcgtcgccg aagcgtccac caaggtcaag agcgacatca
     5941 agctcaccga aggcacgcgc atcgagtggg gtggtcagta cgaaacgctg cgcgcggcgc
     6001 agcagcgact catgttggtc attccggcgg tgctcctgct gatcgtcttc gtattgttcc
     6061 tgcacttcgg cgaggcgggt cccgtcgcgt gggtgctctc gcacgtaccc ttcgcggcgg
     6121 tggggggaac cctcgccttg ctcgcgcgag gcatgccgct ctcgatctcc gctggcatcg
     6181 ggttcattgc gctgtggggc atcgcggtca tgaacggcac ggtactcgtc acagaaatct
     6241 tggagctcga gcgccgcggg ttcgcgccgc gcgaggcgac gctgaaagcg gccgaagaca
     6301 gagcgcgacc ggtttcgatg accgcgatgg tcgcggcgct cgggttcttg cccatggcca
     6361 tcgcgcaggg cccaggagca gaggttcagc ggccactggc gacggtcgtc attggcggcc
     6421 tcgtgagctc gaccacgctc acgctgctcg tactaccgac gctacgggcc gcgttcgcgc
     6481 aacgtttccg gcgacgacgc gacgcgaaag tcgcggccgc gcctggcgtg cccgaagaga
     6541 tccagagctg agctacgacg actcagaagg cccaggcgac ctgggtgccg agttgcttca
     6601 acggatagcg gctcaaaccg acccgatccg gcttgcccca cgtcgcgagc gaaaagtcga
     6661 ccttgacgac gacgtctgga atgggccgaa tgttgaggcc gatgtcgaag cccgtggggg
     6721 gcggcaggta gagatcgtcc ggcttctgcg cgtgt
//
LOCUS       DASTKS010000040         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1788671, whole genome shotgun sequence.
ACCESSION   DASTKS010000040 DASTKS010000000
VERSION     DASTKS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788671"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VFQ35_00865"
     CDS             complement(<1..1406)
                     /locus_tag="VFQ35_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HET9929199.1"
                     /translation="MKIETLASGTCNVGVHRTTLTPAEAYAMAGYSWAGRIGTPSGDE
                     DLFARALYLESAGECVALCFIDLMSASLRLVQAVAERLKKAGEATLAENFILSGTHTH
                     TGPGNFYGNSFYDVFAQNLFGAERKGFQAGLVAQMADAICRSILGAKQNARSGRLVVN
                     RAEVWGLSRNRSLPAYAANFFNGAGERITLGGTPPAELSEAECAIDPRLTTLTAVTDT
                     QVIGAFSLFGCHATALGPKWETYARDWPGVLVDTVEAGLRERGLCGEGTAVAALGQGA
                     GGDITPLPFDDDRGASQGPELAQRVGAALAKAALACVDQAMSEAALVPLVLSHESGIF
                     RVDAPSGERPWMIGLPVMGGAEDGRSVFYELGFVREGTAQRPDGSPQSPKVPALGVIQ
                     EMLAEVALDIAPYHPWHRLKLGAHVIFSVPGEPTATAAHELETALLSATGHASASVLG
                     YTGDYAGYFTTEAEYECQ"
     gene            1749..2057
                     /locus_tag="VFQ35_00870"
     CDS             1749..2057
                     /locus_tag="VFQ35_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929200.1"
                     /translation="MPRAASELPRSDNWPIPLVVRRQSGGGWREISADWFDAAERTLY
                     PPRNSDVSDDDDGYQLVSRRRLRLAHALFVLLALAVVALIAWEVHVYFGNGTSLPRLR
                     "
     gene            complement(2109..3179)
                     /locus_tag="VFQ35_00875"
     CDS             complement(2109..3179)
                     /locus_tag="VFQ35_00875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9929201.1"
                     /translation="MCSEDSLYKGQAGPKFFEMSALLPLGRVAYQAQQVALRLSLAAA
                     HLGLRPLLPPREKPSRRDLAFLRRRFSRLLARDLENVRAGLYPEELLFSLPVREYLSG
                     LPALAVEVARMARRARSGEVRDLPEEVNLADYPPYFRRNFHWQTDGYLSRRSAELYDL
                     SVEFLFLGAADVMRRQTLPAVTRFLRKNPGQQRILDVACGTGRLLYQLSLAHPTHAYT
                     GLDLSPFYIERARELLSAKPVSLVNGNAETLPFGENSYDAITSVFLFHELPVRARRNV
                     LAEMRRVLRRDGLLVIEDAAQLADSPELRVFLENFGKDMNEPFFLDYLNETLEPMLEE
                     AGFKVESVTPAFLSKVIVARAV"
     gene            complement(3306..>3768)
                     /locus_tag="VFQ35_00880"
     CDS             complement(3306..>3768)
                     /locus_tag="VFQ35_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929202.1"
                     /translation="TSSDASLGGYGYYFKPTKGHSYSLSGWIRAENLPSGAVAQIRLD
                     YIGSTTPVTARDATALAAPIDDYLSWGNRNGVPMYLGEFGVYRACFEGDKGGLNWVSD
                     MLDLLRERSLPFTYHAYHEDAFGIYYGDGTLPDPENANRPLIDLFTAKLGS"
BASE COUNT          688 a         1209 c         1179 g          692 t
ORIGIN      
        1 tgctggcatt cgtactcggc ttcagtcgtg aagtagcccg cgtagtcccc ggtatagccg
       61 agcacgctcg cgctcgcgtg gcccgtcgcc gagaggagcg ccgtctcgag ttcgtgcgca
      121 gccgtcgcgg tgggttcgcc cggaacactg aagatgacgt gagcgccgag cttgagtcga
      181 tgccacgggt gatagggcgc gatgtcgagc gccacctccg cgagcatttc ttgaatgacc
      241 ccgagcgccg gtaccttcgg gctctggggg ctcccgtcgg gtcgttgcgc ggtgccctcg
      301 cgcacgaatc cgagctcata gaagacgcta cgaccgtctt cggcccctcc catcaccgga
      361 agtccgatca tccaagggcg ctctccactc ggcgcatcga cgcgaaagat gcccgactcg
      421 tggctcagta cgagcggcac caacgccgcc tccgacatcg cttggtcgac gcacgctaac
      481 gccgccttcg ccagtgccgc gccgactcgc tgcgcgagct ccggaccctg agaagcgccg
      541 cggtcatcgt cgaaaggcag cggcgtaatg tcacctcccg cgccttgtcc cagtgccgcc
      601 accgcggtgc cctcaccgca gagcccgcgc tcgcgaagac ccgcttcgac cgtatcgacg
      661 agcactccgg gccagtcccg ggcgtacgtt tcccacttgg gcccgagcgc cgtcgcgtga
      721 cagccgaaca gcgaaaaggc gccgatgact tgcgtgtcgg taaccgcggt gagagtcgtc
      781 aatctaggat cgatggcgca ctcggcttcg ctcagttctg ccggaggagt tccaccgagc
      841 gtgatgcgct cgcctgcgcc gttgaagaag ttcgctgcgt acgcggggag gctacggttg
      901 cgcgagaggc cccagacttc cgcgcgattt acgacgaggc gaccgctgcg cgcgttctgt
      961 ttggcgccga ggatgcttcg acaaatcgcg tcggccatct gcgccacgag gcctgcctgg
     1021 aacccctttc gttcggcgcc gaacagattt tgagcgaaca cgtcgtagaa gctgttgccg
     1081 taaaagttgc ccggtcccgt gtgcgtgtgg gtaccagaca gaatgaagtt ttcagcgagc
     1141 gtcgcttcac cggctttttt gagtcgctcg gcgacggcct gcacgagccg gagcgatgcg
     1201 ctcatcaggt cgatgaaaca cagggcgacg cattcacccg cggattccag gtagagcgcc
     1261 cgtgcgaaca aatcttcgtc gccgctcgga gtaccaattc gccccgccca cgaatacccc
     1321 gccatggcgt aggcttccgc cggcgtcagc gtcgtccgat gcactcccac gttgcacgtg
     1381 cccgacgcta aggtttcgat tttcatcgcg attcttctcg gtatcgcagc gtcttcggcg
     1441 gcgcaatggc gataacttcg gcgaaacgcg gatcaaccag atacgaatcg aattccaagc
     1501 agcgtggacg gcctctacgc ggcatcgaat ctgcaagcgt gaatgggtgg cctttctgcc
     1561 agaaacgcgg caaaacttac gggcgctcga ccttggggcg agcgcgttgc ctgtgtggcg
     1621 ggccttaccg agtactagag tctgcgcaag ccgcgcgctt gccgataccg aaggggatgc
     1681 ttatgggccg accgaacact gaaacagcga ctttcggctg gttggggccg cctgaaggtt
     1741 cgagcgaaat gccgcgcgcc gcgagcgaat tgccgcgctc tgacaactgg ccgatccctc
     1801 tcgtcgtgcg acgacagagc ggcggcgggt ggcgagagat cagcgctgac tggttcgatg
     1861 cagccgagcg aactctgtat ccacctcgaa acagcgacgt gagtgacgac gacgacggct
     1921 atcagctcgt ctctcgccgg cgtttgcgcc tcgcgcacgc gttgttcgtt cttttggcgc
     1981 tggcggtcgt cgcgctcatc gcgtgggaag tgcacgtcta cttcgggaac gggacttcgc
     2041 ttcccaggct gcgctgaacg cgcctgtggc gcctcgcgcc tcacgcccag tggcgcgcgc
     2101 cgagtcgttt acacggcgcg cgcaacgatg accttggaga ggaacgccgg ggtcacgctc
     2161 tccaccttga accccgcttc ttcgagcatc ggctcgagcg tctcgttcag atagtcgagg
     2221 aagaacggct cgttcatgtc cttcccgaag ttctcgagga agactcgcag ctcgggcgaa
     2281 tcggcgagtt gtgccgcgtc ttcaatcacg agtagcccgt cgcggcgcag tactcgtcgc
     2341 atctcggcca aaacgttccg acgcgctcgg accggaagct cgtggaacaa gaagacgctc
     2401 gtgatggcgt cgtagctgtt ctctccaaat ggcagcgtct ccgcgtttcc gttgacgagc
     2461 gaaaccggct tcgcggagag cagctcacgc gcgcgctcga tgtaaaacgg gctcaaatca
     2521 agccccgtat acgcatgggt cgggtgcgcc aagctcagtt ggtagaggag cctgcccgtc
     2581 ccgcaagcga cgtccagaat tcgctgctgg cccgggttct tacgtagaaa gcgcgtgact
     2641 gccggcaacg tctgccgtcg catgacgtcc gccgccccga ggaacaggaa ctccaccgaa
     2701 agatcgtaca gctccgccga gcgccggctc aggtacccgt ccgtctgcca gtggaaattc
     2761 cgtcgaaagt acggagggta atccgcgagg tttacttctt ccggtaagtc gcgcacctcg
     2821 ccactccggg ctcgccttgc catgcgcgca acctccacgg caagcgccgg cagcccagac
     2881 aggtactcgc gtacgggtag gctgaacaac agttcctctg ggtagaggcc ggcccgcaca
     2941 ttctcgaggt cccgcgcgag caggcgagaa aagcggcgcc gcaagaacgc gagatcgcgc
     3001 cggctcggct tctcacgcgg gggtagcagc ggacgcaatc cgaggtgcgc cgcggcgagc
     3061 gataggcgca gcgcaacttg ctgtgcctgg taagcgactc gaccgagagg aagcaacgcc
     3121 gacatctcga agaacttggg gcctgcttgg cccttgtaaa gtgagtcctc cgagcacacg
     3181 gtgtgaactc atactgcgaa ctgcgctgcg cacctttttg tcgccgctct ggcgccacgc
     3241 gcatcgatac ggggctgtgt gacgccgaac ggcccggact aacgaccaac cccaggctaa
     3301 aggagctaag agcccaactt ggccgtgaat aggtcaatca atgggcgatt ggcattctct
     3361 gggtccggta acgttccgtc gccgtaatag atgccgaacg catcttcgtg gtaggcgtga
     3421 tacgtgaacg gtagggagcg ttcgcgaagg aggtcgagca tgtccgacac ccagttgagc
     3481 ccgcctttgt ctccttcgaa gcacgcccga tagacgccga actcacccaa gtacatcgga
     3541 acgccgttgc ggttacccca gctcaggtaa tcgtcgatcg gcgcggcgag cgccgttgcg
     3601 tcgcgcgcgg tgaccggcgt ggtactgccg atgtagtcga gtcgaatctg tgccactgca
     3661 ccgctgggca gattctccgc gcgaatccag ccgctcagcg aatacgagtg acccttggtt
     3721 ggcttgaagt aatagccgta acccccgagg ctcgcatcgc tgctggtt
//
LOCUS       DASTKS010000041         8345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1120286, whole genome shotgun sequence.
ACCESSION   DASTKS010000041 DASTKS010000000
VERSION     DASTKS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120286"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..4828
                     /locus_tag="VFQ35_00885"
     CDS             53..4828
                     /locus_tag="VFQ35_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9929203.1"
                     /translation="MAEDRYQSVQGILRDVDECERRLSAGDDGSFALARDEATPRLTL
                     PQRVVGRTEEQQRLMLALEQAAAGVPRFVFLSGAAGVGKTTLANQLEAPTLARGGCFV
                     SGKFDVLARSQPYSALADGLSRWFARLASEDGERCDAIARTLADTLGENAATLAALVP
                     GLGVSRGDGAAPPRLGPIEAQNRLALSVERLLAALARVGSPLVILLDDLQWADLATLR
                     LIGNVFANAEPLPILWVGAFRDNEVPVEHPVLRLRRELQRREGAIVDLNVGPLSSEAV
                     GELLREALHTKSSELDGLARLVLSKTLGNPFFVLQFVRSLERDGLLSFDRARGAWAFD
                     PVRIEALEMTDNVVELMTQKLGYLGPLTQRATQCAACIGATFSLDTLAEVLGRDQRSA
                     AQDLWEALLEGLVLPLHPNYELLLVSDTIEQEHIELRFLHDRVQQAAYDSLAEGARAE
                     LHLLIGRSLARRADAATRLFEIVGQLNRARSSMTQASEREQLAALNLEAGQRARAAAA
                     IPVALSLFLVGLELCGDRSSKTFHELTLGAAECEFITADAATAEERLDWLLASAAPLE
                     LRVEALRVRIVRYENVGRFAESVAVGVRALELLGVRLPAEPEGRSAALEDELAEISKR
                     LSSRGIASLFELPIAVDPHAHLVSGIIMAMWPSAFLTNPGGLTALLSASLVRRSLEHG
                     NTAESALGYVTHAITTNARTGDYASGRAFAELGLRLSAGRGHKPLALKVEHLYGSFLS
                     SLYEPLARGAEHAEVAHRAALECGDFTYAARAAFMGTWYDFFGGVPLDVFDTRVGTAL
                     DFMRRVRHEMIEHATSLLLQCSRCLQGLTRGTTSLSDSAFDEPRIAEQLQKVPIFTGF
                     LETVRAFLQYAFGDFAAAEQHATNARAALAGSAETLFHFELEFYSALTQAALATPESY
                     AQRVAAVDAVLTRLERRAATCNKNFGYQLELLLAERARILSEPDRAHRHYARALELTS
                     AEDLCAQASCRERFAEFLLQIGDAVAARRELSAALGAYEKWGAHAKVRQLRERHAPRL
                     TDEPAAPLTDFDVLSTLKAAQAIAAEIERNRLLPRLLRILIENAGAERGVLLEPSAEG
                     LVIVAEQRVGEPHSDSSVERIASSEEVPLELLEGVARTAEPLLARNASEDPALRDDPY
                     VRRRASRSLLCLPLVSQGSVRALAFLENSLASEVFSDERLSAARVLAGQAAISLENAR
                     LYEAMRKEVERRSAAERALTTALRDVDALKNRLQAENVYLQEELQSHHNFEEIVGKAP
                     DLLVALKLVESVANTDSTVLVLGETGTGKELFARAIHSRSARRERPLVKVNCGAIASG
                     LVESELFGHVKGAFTGALAKRVGRFELADGGTIFLDEVGELPLDTQAKLLRVLQEREF
                     EAVGSSTTQRVDVRVIAATNRDLEQAVRAGRFRADLLYRLNVFPIVVPPLRRRAEDIP
                     LLVSFFLTRLAGRLGKKLEGFSRAEMQLLRDYPWPGNVRELENVVERAAILAPGPLLS
                     LDAHFGRERGTAPEVRPDLRAGLRVADPAAPANIPRSGVRSMDEVERQHVLQVLESAS
                     WVIEGERGAAKLLGLHPNTLRSRMKKLGISRRAHSGSSG"
     gene            4979..5239
                     /locus_tag="VFQ35_00890"
     CDS             4979..5239
                     /locus_tag="VFQ35_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929204.1"
                     /translation="MLRISHSESPDGPHLRLEGRLVGEWVLVLAKELEALGRYPALVL
                     DLASVEFVNEAGVRVLLDARARGARFEACSPLLTSLLGDDPR"
     gene            5236..5865
                     /locus_tag="VFQ35_00895"
     CDS             5236..5865
                     /locus_tag="VFQ35_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929205.1"
                     /translation="MSAAERSAATPFGAPSEAESNSAIPLLDLAMSRYADGDASAFNA
                     VFRGLAPRIRAFLQRLSGRADVADDLTQETFLRMHRARGSFTHGRSVVPWAYAIARNC
                     YIDHARVKAKLSLSPTDISELEVAADPSSCAEEIAVAKQSARTVEQVLATMTVARREA
                     FILLRYEGMSVETAAAVLGISEGAVKLRAFHAYELLRAALNPSSREAAS"
     gene            5862..6563
                     /locus_tag="VFQ35_00900"
     CDS             5862..6563
                     /locus_tag="VFQ35_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9929206.1"
                     /translation="MSEPFDFSKLGELGEIPDPLSASPPEPHFRDFRSSSDGPDRTTT
                     RRRRWIALALSGAWLGGHLLVFGVRGDLSKLPVAYVVGQIALPFVLAVASLWVALTRG
                     RAGLGARVVALVTLSFVGPVAFWAVATLAPSPRPALAGEASGVRVLLCLDLTLLWTAV
                     PLLCAALVLRHAFPAASRWRSALLAVAAGLLASGVMNLHCPNVTKLHIAVGHGLPILI
                     ALVVGAILMPRATRA"
     gene            6706..>8345
                     /locus_tag="VFQ35_00905"
     CDS             6706..>8345
                     /locus_tag="VFQ35_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9929207.1"
                     /translation="MTSSAGTRDDEPIDVARSLRNSDQLFRVLVEQVSEYAIFLLDAE
                     GYVQTWNRGAERIKGYTLSDIVGKHFSTFYRPEDRWKCAEEIAVATREGRVEDEGIRV
                     RKDGSLFWANVVITCLRDPSGRIIGFAKVTRDMTERQRAAESLRQSEERFRLLVESVK
                     DYAIFMLDPSGVVATWNVGAQRIKGYRADEIVGQHFSRFYPEAEVRAGKCEFELVEAA
                     RVGRFEDEGWRIRKDGSRFWANVVITALRGPEGELRGFAKVTRDLTERRAAEQENIRL
                     AQVARERVHTLAALSESLAGALSVADVGRAVCSMGRDFAGGDTITLYLHDARDNSLEL
                     VAERGCNPALLARLRRIDADSGNPCHAIGLGTAESVWIRNEAEYRAFFPSLANTAVTG
                     DRARAFACVPLLAEGRTIGMLGLGFHQPHDFTQEQREFIDTFARQCAQALARARRVET
                     ERAAAELAERLRASLATTLRSIGDAVIATDPRGHVTLMNGVAEDLTGFKEEEAHGKPL
                     PEIFHIVNEHTRALVQNPVEKVLETGGIVGLANHTVLIA"
BASE COUNT         1359 a         2802 c         2737 g         1447 t
ORIGIN      
        1 cgcgagcgtt cccgagagcc tctcgcggct cgtcctcagg ctgctcgaga aaatggcgga
       61 ggaccgctat cagagcgttc agggcatttt gcgagacgtg gacgaatgcg agcgccggct
      121 gagcgcgggc gacgacggct cgttcgcgct agcgcgggac gaagcgactc cacgcctgac
      181 tttgccgcag cgggtggtcg ggcgcaccga agagcagcag cgtctgatgc tagcgctcga
      241 gcaagccgcc gcgggcgttc ctcgcttcgt ttttctcagt ggggcggcgg gcgtcggcaa
      301 gaccacgctc gcaaaccagc tcgaggcgcc gaccctcgcg cgcggcggtt gcttcgtctc
      361 tggcaagttc gacgtactcg cgcgcagcca gccgtactcc gccctagcgg atggcttgtc
      421 tcgttggttc gcacgactcg cgagcgaaga cggcgagcgc tgcgacgcga tcgcaaggac
      481 tctcgccgac acactgggcg agaacgccgc aaccctcgcg gcgctcgtgc ccggcctcgg
      541 cgtctcacgc ggagatggcg cggcgccgcc tcgactcgga cccatcgaag cgcagaaccg
      601 gctcgcgctc tcggtcgagc ggttgctcgc ggcgttagcg cgcgtcggct cgccgctcgt
      661 cattttgctc gacgatctgc aatgggcgga ccttgcgaca cttcgcctga tcggcaacgt
      721 gttcgccaac gccgaaccgc tcccgatttt gtgggtgggt gcgtttcgcg acaacgaagt
      781 tccggtcgag cacccggtgc tgcgcctgcg ccgagagctg cagcgtcgcg aaggcgcgat
      841 cgtggacctc aacgtcgggc cgctttcatc cgaggcagtg ggtgagctgc tgcgcgaggc
      901 gctccacacg aagagcagcg agctcgacgg gctcgctcgt ttggtcctgt ccaagaccct
      961 cggaaacccc ttcttcgtgc tccagttcgt gcgttctctc gagcgagacg ggctgctctc
     1021 cttcgatcgc gcccgcggcg cttgggcgtt cgaccccgtg cgcatcgaag cgctggagat
     1081 gaccgacaac gtggtcgagc tgatgacgca gaagctcggc taccttgggc ccctcacgca
     1141 acgcgctacc cagtgcgcgg cctgcattgg tgcgaccttc tcgctcgaca ccttggcgga
     1201 ggtgctcgga cgcgatcagc ggagcgccgc gcaagatctc tgggaagccc tgctcgaagg
     1261 gctcgtctta ccattgcacc cgaactacga gctcctactc gtctccgaca caatcgagca
     1321 ggagcacatc gagcttcggt ttctgcacga tcgggtgcaa caagctgcct atgactcact
     1381 tgccgagggt gcgcgcgccg agcttcacct cctcattggc cgtagcctcg cgcgccgcgc
     1441 agacgcagcc acgcggctgt tcgaaatcgt ggggcagctg aaccgcgctc gcagctccat
     1501 gacccaggcg agcgagcgcg agcagctcgc cgcactgaac ctcgaagcgg gtcagcgggc
     1561 ccgcgcggcc gcggcgattc ccgtagcatt gagcctattc ttggtaggac tcgagctgtg
     1621 cggcgatcgg tcctcgaaga catttcacga gctcacgctc ggggccgcgg agtgcgagtt
     1681 catcaccgcc gacgcggcta cggccgaaga gcggctcgac tggctccttg cctccgccgc
     1741 cccgctcgag ctccgggtcg aagcgctgcg cgttcgcatc gtccggtacg aaaacgtcgg
     1801 tcgctttgcg gagtcagtcg ccgtcggcgt tcgagctctc gagctcctcg gggtgcgctt
     1861 gccggccgag cccgaaggac gtagcgccgc gctcgaagac gagctcgccg aaatatccaa
     1921 gcgactctcg tcgcgcggca tcgccagtct gttcgagctc cccatcgccg tcgacccgca
     1981 cgcgcacctc gtttcgggca tcatcatggc gatgtggccg tccgcgttct tgacgaaccc
     2041 gggcggcctt accgcgctgc tctcggcaag cctcgtgcgg cgctcgctcg agcacggcaa
     2101 cacggccgag tcggcgctcg gctacgtgac tcacgccata acgacgaacg cgcgcaccgg
     2161 cgactacgcg agcggacgtg ctttcgcaga gctcgggctt cgattgagcg cggggcgcgg
     2221 gcacaaaccg ttggcgctga aggtggagca cctttacggt agcttcttgt cctcgctcta
     2281 cgagccgctc gctcggggcg cagagcacgc cgaggtcgcg catcgtgctg cactcgagtg
     2341 cggtgacttc acctacgccg ctcgcgccgc cttcatgggt acctggtacg actttttcgg
     2401 cggtgtaccg ctcgacgtgt tcgatacgcg tgtcggaacc gcgctcgact tcatgcggcg
     2461 cgtccgtcac gaaatgatcg agcatgcgac gtcgctgctc ttacaatgtt cgcgttgttt
     2521 gcaagggttg acacgcggca cgactagcct ctcggacagc gcgttcgacg agcctcgcat
     2581 cgccgaacag ctccaaaagg ttccgatttt cacgggtttc ctcgagaccg tgcgcgcgtt
     2641 cttgcagtac gcgttcgggg atttcgcggc ggccgagcag cacgcaacga acgctcgggc
     2701 ggccctggcc ggctcggccg agacgctctt tcacttcgag ctcgagttct actcggcgtt
     2761 gacgcaagcc gcgctcgcaa ccccggaaag ctacgcgcag cgcgtcgccg cggtcgacgc
     2821 cgtactcacg cggctcgagc ggcgcgcggc aacgtgtaac aagaacttcg gctaccagct
     2881 cgagctgctc ttggccgaac gagctcgaat cctttctgag cccgaccgag ctcaccggca
     2941 ctacgcgcgc gctctcgaac taacgagcgc ggaggatctc tgcgcccagg cctcgtgccg
     3001 cgagcgcttt gcggaattcc tgctccaaat cggggacgcc gtcgccgcgc ggcgggagct
     3061 ctcggcagcg ctcggcgcgt acgaaaaatg gggagcgcac gccaaggttc gacaactacg
     3121 tgaacggcac gcaccacggc tcacggacga gcccgccgca cccctcaccg atttcgacgt
     3181 gctgagcacg ctgaaggcgg ctcaagccat cgcagcggaa atcgagcgca atcgattgct
     3241 cccccgactc ctgcgaatcc tcatcgagaa tgcgggcgcg gaacgcggcg tgctcctcga
     3301 gccaagcgcc gaaggcctcg tcatcgtcgc cgagcagcgc gtcggcgagc cccactcgga
     3361 ctccagcgtc gagcgcatcg cgtcctccga agaagtcccg ctcgaactcc tggagggcgt
     3421 cgcgcgcact gccgagccgt tgctggcgcg caacgcgagc gaagaccccg cgctccgtga
     3481 cgacccctac gtgcgacgtc gtgcgtcgcg ctcgttgctg tgcttgcctc tcgtgtcgca
     3541 agggagcgtg cgcgctctcg cgttcttgga gaactcactc gcctcggagg tgttctcgga
     3601 cgagcgactt tctgcggcgc gcgtgctcgc tggccaagcc gcgatctcgc tcgagaacgc
     3661 tcgcttgtac gaggcgatgc ggaaagaagt cgagcgtcgc agcgccgccg aacgagcgct
     3721 caccacggcg ctgcgcgacg tggacgcact gaaaaatcgc ctccaggccg agaacgtcta
     3781 cctgcaagaa gagctgcaga gccatcacaa cttcgaagaa atcgtcggca aggcgcccga
     3841 cctcctcgta gccctgaaac tcgtcgagag cgtcgcaaac accgactcga ccgtgctcgt
     3901 gctgggcgag accggcactg ggaaagagct gttcgcgcgc gccatccaca gccgcagcgc
     3961 gcggcgggag cgaccgctcg tcaaagtcaa ttgcggagcc atcgcgagcg gactcgtcga
     4021 gagcgagctc ttcggacacg tgaagggcgc cttcaccggc gctctcgcga agcgcgttgg
     4081 tcgtttcgag ctcgccgacg gcgggaccat cttcctcgac gaagtcggcg agttgccgct
     4141 cgacacccaa gcgaaactgc tgcgcgtgct gcaagaacgg gagttcgagg cagtcgggag
     4201 cagcacgacc caacgcgtcg acgtccgcgt gatcgcagcg acgaaccgcg acctcgagca
     4261 agccgtgcgc gctgggcgct ttcgcgcgga tctgttgtat cgcttgaacg tctttcccat
     4321 cgtcgtcccc ccgcttcggc ggcgagccga agacattcca ttgctcgtgt cgttctttct
     4381 gactcgcctg gcgggccgtt tgggaaagaa gctcgaaggg ttctcgcgcg cggaaatgca
     4441 gctgcttcgc gactatccgt ggccgggcaa cgtgcgagag ctcgagaacg tcgtcgagcg
     4501 agctgcgatt ctggcccccg gcccgttgct gagcctggac gcgcacttcg gtcgcgagcg
     4561 gggcacggcg cccgaagttc gacccgactt gcgagcgggg ctccgcgtcg ccgatcccgc
     4621 cgcgcccgcc aatatcccga ggtctggggt tcgctcgatg gacgaagtcg agcgccagca
     4681 cgtcctacaa gtgctcgaaa gcgcgtcctg ggtcatcgag ggagagcggg gcgcggcaaa
     4741 gctgctcggg ctacatccca acacgctgcg aagccggatg aagaagctcg gtatcagccg
     4801 acgagcgcat tcgggctctt ccggctgagg ttcgtggcca cgacatttcg tggggcgcgc
     4861 tccgagacac ccacgaaata tcgtggcccg cctccgtccc cgagaactcg ttatccgttt
     4921 ccctacggat ttcagcgata ctctcggcgg cacgaggctt gcgtagcgct cgctcgccat
     4981 gcttcgcatc agccatagcg agagcccgga tgggcctcat ttgaggctcg agggacgcct
     5041 cgtgggggag tgggtgctgg tgttggccaa ggagctcgag gcgctcggac gctacccagc
     5101 tttggttttg gacttggcgt cggtcgagtt cgtgaacgaa gccggcgtgc gtgtgctgct
     5161 cgacgcccgc gcacgcggcg cgcggttcga agcgtgttcg cctcttctca ccagcctatt
     5221 gggagacgat cccagatgag cgcggcagag cggtcggcgg ctactccatt cggcgctccg
     5281 tcggaggcgg agtcgaacag cgccatcccg ctgctcgact tggcgatgag ccgctacgct
     5341 gacggggatg cttccgcctt caacgccgta ttccgcggac tcgcgccgcg catccgcgcg
     5401 ttccttcaac ggttgagcgg tcgagccgat gtggcggacg acctgacgca ggaaacgttt
     5461 ctgcgtatgc accgtgcgcg ggggtccttc acgcacggcc gcagcgtcgt gccctgggct
     5521 tacgccatcg cgcggaactg ttacatcgat catgctcggg tcaaagcgaa gctttcgctt
     5581 tcgccgaccg acatttccga gctggaagtg gcggccgacc caagctcttg cgcggaggaa
     5641 atcgcagtcg caaagcaaag tgcgcgaacc gtcgagcagg tgctcgcgac gatgacggta
     5701 gcgcgtcgcg aagccttcat cttgctgcgc tacgaaggca tgagcgtcga aaccgccgcg
     5761 gccgtcctcg gcatctccga gggcgcggtc aaactgcgcg ccttccacgc ttacgagctg
     5821 ctccgagcgg cattgaatcc atcctctcga gaggcagcgt cgtgagcgaa cccttcgact
     5881 tcagcaagct cggcgagctg ggcgaaatac cagacccgct ctcggcctcg ccccccgaac
     5941 ctcacttccg agactttcgc tcgagctcgg atgggcccga ccgaaccacg accaggcggc
     6001 gtcgctggat tgcgctcgca ctctcgggcg cgtggctcgg cggtcaccta ctcgtattcg
     6061 gggttcgtgg agatctgagc aagcttccgg tcgcgtacgt agtcgggcaa attgcgctgc
     6121 cgttcgttct ggcggtagcc tcgttgtggg tcgcgctcac acgcggtcgc gctggactcg
     6181 gagcgcgcgt cgtcgcgctg gtcacgctct cgttcgtcgg cccggttgct ttttgggctg
     6241 tcgcgaccct ggcgccctcg cctcgcccgg ccctcgccgg cgaagcatct ggggtccgcg
     6301 tcctgctttg cctggacctc acgctgcttt ggacggccgt cccgttgctt tgcgcggcgc
     6361 tcgtgctgcg gcatgcgttt ccggcagctt cacgttggcg tagcgctttg ctggcggtgg
     6421 cggccggact gttggcctcc ggcgtcatga acctgcattg cccgaacgtg accaagcttc
     6481 acatcgcggt tgggcacgga ctcccgatcc tgatcgcgct cgtagtcggc gcgatcctga
     6541 tgccgagagc gacgcgcgcc tgacctggca ccggggaacg ttggaatggg cgagtttgcc
     6601 ggctgtggca gatttgcccg cgccgtcgag tgacgcaccg cgcgtgacgc gcgacgctgc
     6661 tcgctagatg tcgcacggcg acaggtcgcg cactaaattg cggacatgac gagctcggca
     6721 ggcacccgcg acgatgaacc catcgacgtc gcgcgatcgc tccgcaacag cgaccagctg
     6781 ttccgggtgc tcgtcgagca ggtaagcgag tacgcaatat tcttgctcga cgcggaaggc
     6841 tacgtgcaga cgtggaaccg cggcgcggag cgaatcaaag gctacacgtt gagcgatatc
     6901 gtcgggaagc acttctcgac gttctatcgc cccgaagatc gctggaagtg cgcggaggaa
     6961 attgcggtcg cgacgcgcga gggccgcgtc gaagacgagg gcatacgcgt tcgcaaagac
     7021 ggttcgttgt tctgggccaa cgtcgtgatc acctgcctgc gggatccgag tggccgcatc
     7081 atcggcttcg ccaaggtcac gcgcgacatg accgagcggc agcgcgctgc cgagtcgctc
     7141 cgccagagcg aggagcggtt tcgcctcctc gtcgagagcg tcaaggacta cgcgatcttc
     7201 atgctcgacc cgagcggcgt cgtcgcgacg tggaacgtcg gcgcgcagcg catcaaaggc
     7261 taccgcgcgg acgagatcgt ggggcagcac ttctctcgct tttacccgga ggcggaagta
     7321 cgcgccggca agtgcgagtt cgagctcgtc gaggcggccc gcgtcggtcg cttcgaagac
     7381 gagggctggc gcattcgcaa ggacggcagt cgcttctggg ccaacgtcgt catcaccgct
     7441 cttcgcgggc cggagggtga gctccgaggg ttcgccaagg tcacgcgcga tctcaccgag
     7501 cgacgcgccg cggagcagga gaacatccgg ctcgcgcagg tcgcccgcga gcgcgttcac
     7561 acgctcgccg ccctctccga gagcctcgcg ggagcacttt cggttgcgga cgtcggccgc
     7621 gccgtgtgct cgatgggccg ggatttcgcg ggcggtgaca cgatcacgct ctacttgcac
     7681 gacgcgcgcg acaactcgct cgagctcgtc gcggagcgcg gctgcaaccc cgcgttactc
     7741 gctcggttgc gccgaatcga cgcagactcg ggcaatccgt gccacgcgat tgggctcgga
     7801 accgccgaga gcgtctggat ccgcaacgaa gccgagtacc gagccttctt tccctccctc
     7861 gcgaacacgg ccgtgacggg cgaccgcgcc cgcgctttcg cctgcgtgcc gctgctcgcc
     7921 gaaggccgaa cgatcgggat gctcgggctc gggttccacc agccgcacga cttcacgcag
     7981 gagcagcgag agttcatcga cacctttgcg cggcagtgtg cgcaagccct cgcgcgcgct
     8041 cggcgcgtgg agacagagcg cgccgccgcc gagctcgcgg agcggttacg cgcgtcactc
     8101 gcgacgacgc tgcgcagcat cggtgacgcg gtcatcgcga cggatccgcg cggccacgtc
     8161 acgctgatga acggcgtcgc ggaggacctc accggcttca aggaagaaga ggctcacggc
     8221 aagcccctgc ccgaaatctt ccacatcgtg aacgagcaca cgcgggcgct cgtgcaaaac
     8281 ccggtcgaga aggtgctgga aaccgggggg atcgtcgggc tcgcgaacca caccgtgctc
     8341 atcgc
//
LOCUS       DASTKS010000042         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1788917, whole genome shotgun sequence.
ACCESSION   DASTKS010000042 DASTKS010000000
VERSION     DASTKS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788917"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1055)
                     /locus_tag="VFQ35_00910"
     CDS             complement(177..1055)
                     /locus_tag="VFQ35_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9929208.1"
                     /translation="MLNFNHLYYFHVTAVEGSVKAAADRLGVTQPTVSEQIRMLERSL
                     GFQLFERTTGGLRLTERGRQVFEHTTAMFLAGERLVDALGQHNSEPISLRVGVSASVS
                     RTVAADFLMPVLSMEQCRPHIRTGDFNDLLRDLRAHEIDLLVGDTEPIEAARPDIELS
                     VLHRPVLVAIVHPEAEPNDNWQNLSLLEYRTASAYHWEVDNFLRDRGLQPTSMGELDD
                     AFLMLEAVARGGFVAFVPSSVARAGIRRGRVKGIAHFTPSSAGVHALFHKSDKSSLAR
                     SVVEKLILHAREHFDD"
     gene            complement(1103..1387)
                     /locus_tag="VFQ35_00915"
     CDS             complement(1103..1387)
                     /locus_tag="VFQ35_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929209.1"
                     /translation="MRVLVVLRDPIVVENIRERCSQPMDAGHSVGVCYVPSDQLGLQA
                     VLDAQRRVTQALRHALDKRAEGVPVFVASERDGDRVEDCARAWGATEVRP"
     gene            1848..2249
                     /locus_tag="VFQ35_00920"
     CDS             1848..2249
                     /locus_tag="VFQ35_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929210.1"
                     /translation="MLHQALVFGSMLTAGFALFATAAWIEADRAAFTSSGSNEITPLV
                     THVSALKSEEVKPPVEEEAPMMMLPPVEIVGRRGLAKRRGVPVAVNEPAIATLPCSDW
                     QEVGPQRVDEGTGIGTRRVRSLCLGPAVTER"
     gene            complement(2236..>2674)
                     /locus_tag="VFQ35_00925"
     CDS             complement(2236..>2674)
                     /locus_tag="VFQ35_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9929211.1"
                     /translation="PCEEAFVHQDETLFSTFWRRRIREKWLWTAWAGGPKAVTLAGLS
                     SKDRELAAIEALASALQTAPEVVHAEALALSSHDFSNDAFSRGAYSFIRPGGTGTVME
                     RFEGEPIVLAGEAFDHDYPGTVAGALASGERAAETVLTHLSAR"
BASE COUNT          469 a          831 c          859 g          515 t
ORIGIN      
        1 cgatgcgagt actcgtacgg agagctgcga tgcggtcgac attggcattc tttccgaaag
       61 cggtgaatgc cttggtgctt gctgatttca tgccatcgca ccgctcgtcg cgctgcggcc
      121 caaccacgtg gcacaaatgc aagtgactac aaccgagcct cccctgcgcg ttcggttcag
      181 tcgtcgaagt gttcgcgtgc gtgcaaaatg agcttttcta ctacgctgcg cgcgagcgag
      241 ctcttgtcgc tcttgtggaa cagcgcgtgg acgcccgcgc tcgacggcgt gaaatgagcg
      301 atgcccttca cgcggccgcg ccggatcccc gcgcgcgcga cgctgctcgg cacgaacgca
      361 acgaagcctc cgcgcgcgac cgcctcgagc atgaggaacg cgtcgtcgag ttctcccatc
      421 gaggtcggct gtagaccgcg gtctcgaagg aagttgtcga cctcccagtg ataggccgag
      481 gcagttcggt attcgagcag tgacaggttc tgccagttgt cgttcggttc tgcctcagga
      541 tgcacaatcg cgacgagcac ggggcggtga agcaccgaaa gctcgatgtc gggtcgcgcc
      601 gcttcgatcg gctccgtgtc gccgacgagc aggtctattt cgtgcgcgcg aagatcgcgc
      661 agcagatcgt tgaagtcgcc ggtgcggata tgcggccgac actgctccat gctgagtacg
      721 ggcatgagga aatccgccgc aaccgtgcgc gacaccgagg cgctcacgcc cacccgcagc
      781 gagatcggct cggaattgtg ctggccgagc gcgtccacca ggcgttcgcc ggccaggaac
      841 atcgcggtcg tatgttcgaa aacctgcctc ccccgttcgg ttagacgaag tccgcccgtc
      901 gttcgctcga aaagctgaaa cccgagcgag cgctcgagca tgcgaatctg ctcactcacg
      961 gtcggttgtg taacacccag tcgatctgca gccgctttga ccgaaccctc taccgccgtg
     1021 acgtggaagt aatataggtg attgaagttc aacacggtgg ggaacgtaac agtcgggcgc
     1081 tgtcacgtcg aattggttta ccctatggcc ttacttcggt ggcgccccaa gcgcgcgcgc
     1141 aatcttccac ccggtcaccg tcgcgctcgc tagccacgaa taccggcacg ccctccgcgc
     1201 gcttgtcgag cgcgtgcctc aacgcctggg tcacccgacg ttgcgcgtcc agaactgctt
     1261 gcagaccgag ctggtccgag gggacgtagc agacaccgac gctatgccct gcatccatcg
     1321 gctggctgca ccgctctcga atattttcga cgacgatggg atcgcggagc acgaccagga
     1381 cgcgcatcct ctcggatcaa gcaagaagca aaccgtgtgg ttttcttacg gacgagtttc
     1441 ggattctcgc gagcatcggc gttacgctcg cgaacaaagg ggcaatgttg tctcgcgccg
     1501 cgtcgtcgtt cacgagcgct tcgagcaatc ggcgagcgtc gcgatgtttc attctcgagt
     1561 gcttagatgg gtgcgcggtc gcgagcgcga gcggagcctg ggcgcgtcgc ttcccgcaca
     1621 ccgtgctcgc tagtttgaaa gcagagcggg ctgcacgaat cgccgccctg gcgaaaaccg
     1681 ctggaaaaaa gccgtgcaat tttgtccggc cggggttgcc ctgctgaaaa attgcgccca
     1741 gggttaattg atcgtggcgt ccgtggactg agttccgcgc gtcgaatccg gtcgtcgcgc
     1801 gacatttttt cgggggcgcc gcatcggtcc gtccgaagga gggcaccatg cttcatcaag
     1861 cgttggtttt cggttccatg ctcacggctg gcttcgcgtt gttcgcgacg gctgcctgga
     1921 tcgaagcaga tcgcgcggcg ttcacctcgt cgggttcgaa cgagattacg ccgctcgtca
     1981 ctcatgtttc tgcactcaag agtgaagaag tgaaaccgcc cgtcgaggag gaggcgccaa
     2041 tgatgatgtt gcctccggtc gagatcgtcg ggcggagagg gctcgcgaag cggcgcgggg
     2101 tgccggtggc ggtgaacgaa cccgccatcg caacgctgcc gtgctccgac tggcaagagg
     2161 tggggcctca gcgcgtagac gaaggtactg gcatcggcac gcgtcgcgtg cgttcgctct
     2221 gcctcggccc tgcagttacc gagcgctgag gtgggtgagc accgtttcgg cggcgcgttc
     2281 tccgctcgcg agcgcgccgg cgacggtgcc ggggtagtcg tggtcgaacg cctcgccagc
     2341 gagcacgatc ggttcaccct cgaagcgttc catcaccgtg ccggtgccgc cggggcggat
     2401 gaagctgtac gcgccgcgac tgaacgcgtc gttcgagaaa tcgtgtgaag agagcgcgag
     2461 cgcttcggca tgcactactt cgggcgccgt ctgcagtgcg gacgcgagcg cctcgatcgc
     2521 ggcgagctca cgatctttcg aggaaagccc ggcgagggtg acggctttgg gccctcccgc
     2581 ccacgccgtc caaagccact tctcccttat tcgccgtcgc cagaacgtgc tgaaaagcgt
     2641 ctcgtcttgg tgcacgaacg cctcttcgca gggg
//
LOCUS       DASTKS010000043         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_5984,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000043 DASTKS010000000
VERSION     DASTKS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5984"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..544)
                     /locus_tag="VFQ35_00930"
     CDS             complement(188..544)
                     /locus_tag="VFQ35_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9929212.1"
                     /translation="MLRIFVAGTSSLEPGEAKVFRFRRGRHALEGFVLRTPVGLTAFA
                     NECPHWHVDLDLGTQDFWDPSSGRIFCKNHAALFHPDTGICERGPCVGLSLERFEVDV
                     GAEGAHVSVPDAEPYE"
     gene            complement(550..2292)
                     /locus_tag="VFQ35_00935"
     CDS             complement(550..2292)
                     /locus_tag="VFQ35_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9929213.1"
                     /translation="MAQPAPQKTAGTSRSRRGDDPALGKVIAGRYRLEARIGEGGMGI
                     VYRARHVLIDRVVAVKLIRPDLRGETHLRAWMLREARAANRVDHAHIIDIHDIGETDE
                     GELYLVMEYLVGTPLSSELARGPLPLARALDILEQMGAALTRAHDLGVVHRDLKSDNI
                     LLTQRGGRKDFVKILDFGLAALAHDPRLAPKGAVFGTPEYMSPEQARGEQAGPHSDLY
                     ALGVLFFEMLTGQLPFRASDRETLLEMQRTAPAPRPSSIKKDCHPVAERMVLKLLEKD
                     PRKRYRDGHHLLEELKALQRSQPNTSWEKEGGEAAPVAPPLPPPPRSPVVGEWGTRAG
                     LFSRMVSRAYPSANAPAEVSQALTALWDVAAKANYLEGEVASHTRKLEALERRGRALR
                     AEIGRKVEELAQEESRVLREAAAYGEEEEAARRELTRAQQVAQERVALAEQAERAGKG
                     TRAIFEQAGAATAMVEAQRQWLKTRETRRTAREATGVDLRRQIEELRAQLARYAEALE
                     EDLASGREKVAGRTREGLKYEKTFLDSSHLLVGHLKGKPECRELMNELLSADGAEPEN
                     GNTNVPAARSADPS"
     gene            complement(2644..3375)
                     /locus_tag="VFQ35_00940"
     CDS             complement(2644..3375)
                     /locus_tag="VFQ35_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929214.1"
                     /translation="MRLLSSVLTCAVTFAWLANAGVAKAEEASPNGKGIAGGALLGAE
                     LVMDVEAALKVKSPWAYLGGGLAGAAAGGVGGYFVEQSVDSARIPMLMLAGGITLAIP
                     TTVAVLSASAYEPPATYVIDQQPADEPVADPARATTPAPAPAAPTPPPATAPNDTTPP
                     ATTPAPSAPPPGPGTPSGALRGRHIAKRSAPRRLPPALIDVDPNQVSISVPNVEVREV
                     FTRAEVAMGAPRATEVRIPVLNVLF"
     gene            3545..>4088
                     /locus_tag="VFQ35_00945"
     CDS             3545..>4088
                     /locus_tag="VFQ35_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929215.1"
                     /translation="MAVFKLLATYALVLLATLSISAWRLNELTLGSAARGAWLPFLRP
                     LIGAGFEVSLLLAVPAALLVARAAGLPRVASAIAFVLLAGVGVWGAVRFDPGAVAPGQ
                     LADALLRQARESCEQAPERKAEVPLLAMRWSCSSSPARLQGRAPIGKRAEFSAANVHL
                     APDLRTISLDELELRVGATLQ"
BASE COUNT          633 a         1350 c         1360 g          745 t
ORIGIN      
        1 ctcgcggacg acttcgccaa ggtcgcggag cctggcatgg cgatggaccc gaccgacagc
       61 acgagcgcat ggcagacggc ggtctcggct ctcagcactt acatcccgat gcgcgcttcg
      121 gccgcgcaga cttccatcaa cagcatctgt cagtgagcgg tcgggccgcg ccgagctcgc
      181 tcggcgctca ctcgtacggt tcggcgtcgg ggacgctcac gtgagcgccc tcggcgccga
      241 cgtcgacctc gaagcgttcg agcgagaggc cgacgcaagg cccgcgctcg caaatgcccg
      301 tgtcggggtg gaacagcgcc gcgtggtttt tgcaaaagat gcgtccgctg ctcggatccc
      361 agaagtcctg ggtgccgaga tccaaatcga cgtgccagtg ggggcactcg ttggcaaacg
      421 cggtgagacc gacgggtgtc ctcaacacga agccttccag cgcgtggcga ccgcgccgga
      481 atcggaatac tttcgcttcg cccggctcga gactcgaggt gccggcaacg aagattcgca
      541 gcacgtcgct cagctcggat ccgccgaacg cgccgcgggc acgttggtgt tgccgttctc
      601 tggctctgcg ccgtcggccg agaggagctc gttcatcagc tcccgacact cgggtttacc
      661 cttcaggtgg ccgacgagca ggtgcgacga gtcgaggaag gtcttttcgt acttcagccc
      721 ttcgcgcgtg cgcccggcga ctttttcgcg gccgctcgcc aggtcttctt cgagcgcttc
      781 ggcgtagcga gcgagctggg cgcggagctc ttcgatttga cgtcgcaggt cgacgccggt
      841 cgcttcgcga gcggtgcggc gcgtctcgcg cgtcttcaac cattgccgct gtgcctcgac
      901 catggccgtg gccgcaccag cttgctcgaa gatggctcgc gtccctttgc cggcgcgctc
      961 cgcctgctcg gcgagcgcga cccgctcctg cgcgacctgc tgcgcgcgcg tgagctcacg
     1021 gcgggcggct tcttcttcct cgccgtacgc ggctgcttcg cggagtacgc gcgactcctc
     1081 ttgcgcgagc tcctccactt tgcggccaat ttcggcgcgg agagcacgac cgcgtcgctc
     1141 gagggcctcg agctttcgag tgtgactcgc gacttcgcct tcgaggtagt tggccttggc
     1201 cgcgacgtcc cacagcgccg tgagcgcctg gctgacctcg gcgggcgcgt tcgcagaagg
     1261 atacgcgcgc gataccatgc gagagaacaa cccagcgcga gtgccccact ccccaaccac
     1321 cggtgagcgg gggggcggag ggagcggagg cgcgaccggt gctgcctcgc cgccctcttt
     1381 ctcccaggag gtgttgggct gcgaccgctg caacgccttc agctcttcga gcagatggtg
     1441 accgtcgcgg tagcgcttgc gcggatcctt ctccaggagc ttgagcacca tgcgctcggc
     1501 gactggatga caatccttct tgatgctcga gggacgcggc gccggagcgg tgcgttgcat
     1561 ctcgagcagc gtctcgcgat cgctcgcgcg gaacgggagc tgccccgtca acatctcgaa
     1621 aaacaacacg ccgagcgcgt agaggtccga gtgcggcccg gcttgctcgc cgcgagcttg
     1681 ctccggcgac atgtactccg gagttccgaa gaccgcgcct ttgggcgcga ggcgaggatc
     1741 gtgcgcgagc gcggcgaggc caaaatccag gattttgacg aagtctttac ggccgccgcg
     1801 ctgggtgagc aggatgttgt cgctcttcag gtcgcggtgc acgacgccga gatcgtgcgc
     1861 gcgcgtcaac gccgcaccca tctgctccaa gatgtcgagg gcgcgtgcga gcggcagcgg
     1921 cccgcgggcg agctcgctcg agagcggagt gccaaccagg tactccatga cgaggtagag
     1981 ttcgccctcg tcggtctcgc cgatgtcgtg gatgtcgatg atgtgcgcgt ggtcgacccg
     2041 gtttgcggcg cgggcttcgc gcaacatcca ggctcgcagg tgagtttcac cgcgcaaatc
     2101 gggccgaatg agcttgaccg cgacgactct gtcgatgagg acgtggcgcg ctcggtacac
     2161 gatgcccatg ccgccttcgc cgatgcgggc ttcgagtcga tagcggccag caataacctt
     2221 gccgagcgcg gggtcgtcac cccgccggct gcgcgaggtg ccagcggtct tctgcggagc
     2281 gggttgtgcc atgttcgtgt gcggtgaagc gctttatcaa cccacgcttc aacagaggat
     2341 gctaacggca ccacccacgc aaagcaaagc ccaaactccg ttttggcata cgcacttgca
     2401 tcggtctgcg agcaatcgtt cgtgtgggag aaatggcgct cacaggaaca ttccgtcgcg
     2461 gactacgtga gtgtgagtcc gttagcgaca tcgtgtgacc tcctcgccaa ttcgcctcgc
     2521 ggtcgcgcga gcccgtcgag gcgaacgcgc acctacgccg gcgcgcgacg ctgcgagcga
     2581 gatgactgcg atatctggcc gtcgctcagg atgcttcgcg cgagctgcgc ggagcgtcgc
     2641 cgctcagaac aacacgttga gcacgggaat gcgcacctcg gtcgccctgg gtgcacccat
     2701 ggcaacctcg gcgcgagtga acacttcacg cacttcgacg ttcggaacgc tgatgctcac
     2761 ctgattcgga tcgacgtcga tgagcgcagg aggaaggcga cgcggagcgg agcgctttgc
     2821 gatatggcgc ccgcgcaaag cccctgaggg cgttcctggt ccaggaggag gagcggaggg
     2881 cgctggcgtc gtcgccggcg gagtcgtgtc gttcggagcc gtggcaggag gcggagttgg
     2941 ggctgccgga gccggagccg gcgtggtcgc gcgcgccgga tctgcgacgg gctcgtccgc
     3001 gggctgttga tcgatgacgt aagtggcagg aggctcgtaa gcgctcgcgc tgagcaccgc
     3061 gacggtggtc ggaattgcga gcgtgattcc acccgccagc atcagcatgg gaattcgcgc
     3121 cgagtcgacc gactgctcga cgaagtagcc gccgacgccg ccagcagccg cacccgcgag
     3181 gccgccgccg aggtaagccc aaggcgattt caccttcagc gcggcttcga cgtccatcac
     3241 cagctcagcg ccgagcaatg ctccacccgc gatccctttc ccgttcggtg acgcctcttc
     3301 agccttcgcc acaccggcgt tagcgagcca ggcaaaggtg actgcacaag tcagaacgga
     3361 cgaaagcaat cgcatcggaa acctctctct acctgccgag gcctctttcg gtgccaagcc
     3421 gccctcggac attcgctgcc gggcgagcat agcatccctt cctctccgct tggccacatc
     3481 ttcccgatgc gctccgccac atcttccaga actggcgcgc gacgtgctca gataggccgt
     3541 tcgagtggcg gtttttaaat tgctcgcgac ttatgcgctg gttttgctcg cgacactctc
     3601 gatttctgcc tggcggctca acgagctgac tctcggttct gcagcacgtg gggcgtggct
     3661 gccgttcttg cgacctttga ttggtgcagg cttcgaggtc agtttgttgc tcgcggttcc
     3721 ggccgcgttg ctcgttgcgc gtgcggcggg tttgccgcgt gtggcctccg ccatcgcgtt
     3781 cgtgctcctc gcgggtgtcg gcgtttgggg tgccgtgcgg ttcgaccccg gcgccgttgc
     3841 ccctgggcag ctggccgatg cgctgctccg acaggcgcgt gaaagttgcg aacaagctcc
     3901 cgagcgcaaa gccgaagtgc cactgctcgc gatgcgctgg tcgtgctcct cgtctccggc
     3961 tcgcctacaa ggccgcgcgc cgatcgggaa acgcgccgag ttcagcgcgg ccaacgttca
     4021 cctcgcgccc gacctccgga ccatttcgct cgacgagctc gagctacgcg tgggcgccac
     4081 cctacagc
//
LOCUS       DASTKS010000044         6984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_674422, whole genome shotgun sequence.
ACCESSION   DASTKS010000044 DASTKS010000000
VERSION     DASTKS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674422"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VFQ35_00950"
     CDS             <1..1466
                     /locus_tag="VFQ35_00950"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HET9929216.1"
                     /translation="LGAVWSSCSPEFGVPSLLDRFRQIEPKLLIAVDGYTYGGRSFDR
                     RAALDEIRAGLPSLAATVCVGEGVAGTLSFAELSSETEPLEFEAVPFEHPLWILYSSG
                     TTGLPKAIVQSHGGILLEHLKALALHLDLGADDRFLWFTTTGWMMWNMLVSGLTVGAT
                     VVLYDGSPAHPNLYALWKLAEEEDVTYFGTSAPYLLACQKAGIEPAQRHRLGPIHTIG
                     TTGAPLPASGFSWVYEHVAPQALLGSLSGGTDVCTAFVLSCPLLPVYAGEIQCAGLGA
                     AVQAFDEHGRPLVDVVGELVLTTPLPSMPIYFWNDPGDARRLDSYFSTYPGVWRHGDW
                     VKHTVRGGYVISGRSDSTLNRGGVRMGTSEFYGVVEALDEIAESLVVDTGTIDDDASG
                     KLWLFVVLRQAVSLDGALTKKIKDAIRNQLSPRHVPDEIRAVPSVPRTLNGKKLEVPV
                     KRILLGVAPEKAAAAGTLLDPKSLEPFIALASELRSA"
     gene            1737..2393
                     /locus_tag="VFQ35_00955"
     CDS             1737..2393
                     /locus_tag="VFQ35_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929217.1"
                     /translation="MANLGVSFLTATVSLSTCLTASFAHAAVGADAAVPANVATSAPD
                     ARTDGEQVGANAKPESSRAAESFNLGGRAFMAPTLGVGALGVGFDETYSVLPNLALGG
                     QYVAYVVDQGADPQYCERCILDGKAALVFAEGRLWPEQWATPYARVGGGLSFVNGQRK
                     AYDSGYGETDVTLLAEAGVELHYRWFSARAFGFRHDILGTKLDRDPLMGFGTQLGVRF
                     "
     gene            complement(2435..3331)
                     /locus_tag="VFQ35_00960"
     CDS             complement(2435..3331)
                     /locus_tag="VFQ35_00960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9929218.1"
                     /translation="MTKKRVRFEAGSLHFGDCLEVLPTLPKATFDLVYVDPPFNIGRS
                     RAARVGNGDRATGTAAYRDNWGGIDGFLAMLEPRVAALAPLLSATGSLWVHLDHRAVH
                     DSKVLLDQVLGRAAYMGEVIWVPGNGARRRHAPSVTHQTILIYAPTGKMYWNSEDEAL
                     REPYAETSLRMHFSELDADGRAFRERVIGGKRYRYYADKGRQIGSVWSDCPAMRANTP
                     LIAETTGYPTQKPEKLLERIVRAASVVGSYVLDPMCGSGTTLAVAARLGRRFVGIDRG
                     EVAFGIAQKRLTKSGSRARAAE"
     gene            complement(3328..4185)
                     /locus_tag="VFQ35_00965"
     CDS             complement(3328..4185)
                     /locus_tag="VFQ35_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET9929219.1"
                     /translation="MIRLRSATALLALCSGCVGVPSPLAPALEGSVGVPHLGAQTSAV
                     ELPLSGPGFVRFRPYGQHHFALPRLVELVSAAAERVATEAPGGAPLVVGDLSARFGGK
                     IQGHNSHRSGRDVDLLLYLTTPSGAPVRSPGFVAIDGDGLGRPASGGQYVRLDVEREW
                     LLVKTLLSSDRAGVQFMFLSKKLEALLIDYAIARGEPAELIYRAETVLLQPTDSTPHD
                     DHIHLRIACAPSEAITGCSGGGPYWDWLPRAETPGDLGPAELLQIAADDPVSDESIAG
                     SSPMVPGGA"
     gene            complement(4182..6797)
                     /locus_tag="VFQ35_00970"
     CDS             complement(4182..6797)
                     /locus_tag="VFQ35_00970"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9929220.1"
                     /translation="MSGRELTKIYEPNPLLEALYRRFFDRIHVDDAWAQQVKTLCEQG
                     RVVYVLRSLNVIDFLALDYLTKRHGLPRIRFVNDLGLWILNPLGKGWLNAILPDSRVT
                     PTDELEDALAHGGSAALFLKRPPGVLDVAAGATGGRGLKEGDELVRVLFELQRKRDEP
                     ILLLPQVFVWSKFPDTRGTEPLDVLIGPREWPSSARTVAQFLYNYKHVTLKLGEPIDL
                     RAFLAESTGQPDSVVVRRVTYAVLRRLERERRVITGPAEKPPERVRQEIVRSPRLRTV
                     IDELADEHTDRYTLQARALEMLAELQATPERTTIKFLEVIFKRLFQRIYAGVEYDSAD
                     LDRLREAAKRGTLVLLPSHKSHIDYLILSYIFNMENLPLPLIAAGDNLNFFPVGGILR
                     RGGGFFIRRSFRGDRLYAAVVDAYVRRLIRDGYPMELFLEGGRSRTGKLIPPKFGILS
                     MIVDAALAVPEPPSLFVPVSIGYERVIEGESYERELQGGEKQKEDAAGLLKSRDVLRH
                     RYGRINIQVGQILSLSEVASELGLDLTSLSPAKRRVLVTRLGNRVMDEINRVTAMTPG
                     ALTALVLLSHDQRGLSHADLLSRSGRLLDVARRLGARVSPALATLNGTLRPESLREAV
                     QMFSDAGLVDMRFPDGLPPNSSGRAGNGAVYSVVEGKRLILDTTKNIIVHFFVERALV
                     ALAVLALGTPEAPLEAVRERVRELSRLFKYEFRFRADAPFEQIFAETIASMESLGELS
                     RTGDVLGPGVGREGWTGSEWLTQYAAILRNFLEGYRVAARSLGALLEKPLADKDMSKK
                     ALATGHHMFLDGEILRWEAVSKPIVASAYSALADLGYLVHREGRYHATPALTSQLVVD
                     EIEQRLVSFLPPRKS"
BASE COUNT         1157 a         2221 c         2375 g         1231 t
ORIGIN      
        1 gcctcggcgc ggtttggtcg agctgttctc ccgagttcgg cgtgccgagc ttgctcgacc
       61 gatttcgtca gatcgagccg aagctcctga tcgcagtgga tggatacact tacggaggtc
      121 gcagcttcga ccggcgcgcg gctctcgacg agattcgggc gggcttgccg tcgctcgccg
      181 ccaccgtctg cgtcggggaa ggcgtcgccg ggacgctctc gttcgccgag ctctcgagcg
      241 aaaccgagcc gctcgaattc gaagcggtgc cgttcgagca tccgttgtgg atcctttact
      301 cctcgggaac gaccggtctc cccaaagcca tcgtgcagag ccacggcggc attctgctcg
      361 agcatttgaa ggcgctcgcg ctgcacctcg acctcggcgc cgacgatcgt ttcttgtggt
      421 ttacgaccac gggttggatg atgtggaaca tgctcgtgag cggcctgacc gtcggcgcga
      481 cggtggtgct ctacgatggc agtccggcgc acccgaacct gtacgcgctg tggaagctcg
      541 cggaagaaga ggacgtcact tacttcggta ccagcgctcc atacctcctc gcttgccaaa
      601 aggcaggaat cgagccggcg caacggcacc ggctgggccc gattcatacg attggtacga
      661 ctggcgcgcc gctgccggcg agcggctttt catgggtcta cgagcacgtg gcgccgcagg
      721 cgctgctcgg ctcgctgagc ggcgggaccg acgtgtgcac ggctttcgtg ctctcgtgtc
      781 cgctgctgcc cgtgtacgcg ggagagattc agtgcgcagg gctcggcgcc gcggtgcagg
      841 ccttcgacga acacggtcgc ccgctggtcg atgtcgtcgg ggagctcgtg ctcacgaccc
      901 cgctgccttc catgccgatc tacttttgga acgaccccgg cgacgcgcgc cgcctcgaca
      961 gctacttctc gacgtacccg ggggtttggc ggcacggcga ctgggtgaag cacacggttc
     1021 gcggcggtta cgtgatctcc ggacgctccg attccaccct gaaccgcggc ggggttcgca
     1081 tgggaacgag cgaattctac ggcgtcgtcg aggctttgga cgagatcgcg gagagcctgg
     1141 tcgtcgatac gggcacgatc gacgacgacg cgagcggcaa gctgtggctc ttcgtcgtat
     1201 tgcggcaggc ggtgagcttg gacggtgcac tcaccaaaaa aatcaaggac gccattcgca
     1261 accagttgtc gccccgacac gtcccggacg agatccgcgc cgtgccgagc gtgccgcgca
     1321 cgctgaacgg aaagaagctc gaggtacccg tgaagcgcat cttgctcggc gtggctccgg
     1381 agaaggcggc ggctgctggt actttgctcg acccgaagtc gctcgagccg ttcatcgcgc
     1441 tcgcgagcga gctgcgcagc gcctgaagac accgccggcg tttgcttcga ttccggcgag
     1501 ggtgatgcag tcgtcgcgcc tcccacctgt gtaacagcgc cgtgccgttg cggcgcaaca
     1561 cagcgtggca gagcgaatac agtgtccgcg cggcgctccg tgacggacag ctgtccgctc
     1621 gaaagttgcg cgatcttcaa cgaaaagccg cgaggcatcg gcaccgtgat ggggcggccg
     1681 cttgcggcat gggcgttgct atcacccgtc cgtccgcatc gatcaaggca gtgctcatgg
     1741 caaacttagg agtttcgttt ctcaccgcaa ccgtgtccct ttcgacttgc ttgaccgcgt
     1801 cgtttgcaca cgcggctgtc ggcgccgatg ccgcggtgcc ggccaacgtg gccacgagcg
     1861 cgcctgatgc acggacggat ggtgagcaag ttggcgccaa cgccaagccg gagtcgtctc
     1921 gcgcggccga gtctttcaac ttgggtgggc gcgctttcat ggcgccaacg ctcggagttg
     1981 gcgcgttggg cgttgggttc gacgaaacct actcggtgct gccgaatttg gcgctcggcg
     2041 ggcagtatgt tgcttacgtc gtcgaccaag gcgccgaccc tcaatactgc gagcgctgca
     2101 tcctcgacgg caaggcggcc ttggtgttcg ccgaaggtcg gctttggcca gagcagtggg
     2161 cgaccccgta cgcgcgggtg ggcggcgggc tctcgttcgt gaatggtcag cgcaaagcct
     2221 acgacagcgg atacggcgag accgacgtta cgttgctcgc cgaagccgga gtcgagctcc
     2281 actatcgttg gttttcggcc cgcgcgttcg gcttccgtca cgacatcttg gggacgaagc
     2341 tcgatcgcga cccattgatg ggcttcggga cccagctcgg cgtgcgtttc taagcggggg
     2401 tcgcacccgc ggttttcgag cgccgaggcg gggactactc ggctgctcgc gcgcggctgc
     2461 cgcttttggt gagtcgtttc tgggcaatgc caaaggcgac ttcgccgcga tcgattccga
     2521 cgaaacgacg tccgaggcgg gctgcgaccg cgagcgtcgt gccggagccg cacatcggat
     2581 cgagcacgta gctgccgacc acgctcgctg cgcgcacgat gcgttcgagc agcttttcgg
     2641 gcttttgggt ggggtagccg gtggtctcgg cgatgagcgg cgtgttggcg cgcatcgccg
     2701 ggcagtcgct ccaaacgctg ccgatttggc ggcccttgtc cgcgtagtag cggtaccgct
     2761 tgccgccaat cactcgctcg cgaaacgctc ttccgtccgc gtcgagttcc gaaaaatgca
     2821 tgcgcaggct cgtctcggcg tatggctcgc gtagcgcttc gtcctcggag ttccagtaca
     2881 tcttgccggt tggcgcgtag atcaaaatgg tttggtgcgt caccgaaggc gcgtggcggc
     2941 ggcgagcgcc gttgccgggg acccaaatga cttcacccat gtaggcggcg cgtcccaaca
     3001 cttggtcgag cagcaccttg ctgtcgtgaa cagcgcgatg atctaggtgg acccagaggc
     3061 tgccagtcgc cgagagcaac ggcgcgagcg cggcgacgcg gggctcgagc atggccaaaa
     3121 agccgtcgat gccgccccag ttgtcgcggt aagcagcggt tccggtggct cggtcgccgt
     3181 tccccacgcg cgcggcgcgg gatcgtccga tgttgaacgg gggatcgacg tacacgaggt
     3241 cgaacgtcgc cttgggaagc gttggcagga cctccagaca atcgccgaaa tgaaggctgc
     3301 ccgcttcgaa gcggactcgt ttcttcgtca tgcgccgccg ggcaccatcg ggctcgagcc
     3361 cgcgatgctc tcgtccgaaa cgggatcgtc ggcggcgatt tgtaagagct ctgccgggcc
     3421 gaggtcaccc ggggtctcgg cgcgcgggag ccagtcccag taggggccgc cgccgctaca
     3481 gccagtgatg gcttcgctcg gcgcgcaagc gatgcgcagg tggatgtggt cgtcgtgagg
     3541 agtgctgtcg gtgggctgaa gcagcaccgt ttcggctcga taaatgagct ccgccggctc
     3601 gccgcgcgcg atcgcgtagt cgatgagcag cgcctcgagt ttcttgctca ggaacatgaa
     3661 ctgcactccg gctcgatccg atgagagcag cgtcttgacg agcaaccact cgcgctcgac
     3721 gtcgagccgg acgtactgcc cgcccgacgc cggacgcccg agcccatctc cgtcgatcgc
     3781 gacgaagccg gggctccgca ccggggcacc agaaggagtc gtcaggtaca agagcaggtc
     3841 cacgtcgcgc ccactgcggt gcgagttgtg tccctggatc tttcccccga agcgcgcgga
     3901 gagatcgccg accacgagag gggccccgcc cggtgcttcg gtcgccacgc gctcggcggc
     3961 ggcgctcacg agctccacca ggcgcggaag cgcgaagtgg tgctgcccgt agggacggaa
     4021 gcgaacgaat ccgggccctg agagcggcag ttcgaccgcg ctggtttgcg cgccgaggtg
     4081 cggcacgccg acgctgcctt cgagtgccgg cgcgagcggc gaggggaccc cgacgcatcc
     4141 actgcagagg gcgagcagag ccgtcgctga acgcaggcga atcatgactt gcgtggtggg
     4201 agaaacgaga ccagtcgctg ctcaatttcg tcgacgacga gctgcgaggt gagcgctggc
     4261 gtggcgtggt acctcccctc gcggtgcacc aggtagccga ggtccgcgag cgcgctgtac
     4321 gcactagcta cgatgggctt gctcaccgcc tcccagcgca ggatctcgcc gtcgaggaac
     4381 atgtggtggc ctgtcgcgag agccttcttc gacatgtctt tgtcggcgag gggcttctcg
     4441 agcagcgctc cgagcgatcg cgccgcgacg cggtatccct cgagaaaatt ccgcagaatg
     4501 gcggcgtatt gggtgagcca ctcgctgccc gtccagccct cgcggccgac tccgggcccg
     4561 agcacgtcgc cagtgcggga gagctctccg agcgactcca tgctggcgat ggtttcggca
     4621 aaaatctgct cgaagggcgc gtcggcgcgg aagcggaact cgtacttgaa gagccgcgag
     4681 agctcccgaa ctcgttcgcg caccgcttcg agcggggcct cgggcgtacc gagcgccaac
     4741 accgcgagcg cgacgagcgc ccgctcgacg aagaaatgca cgatgatgtt cttggtcgta
     4801 tcgaggatca ggcgcttgcc ctcgacgacg gagtaaacgg cgccgttgcc cgcgcggccg
     4861 ctcgagttcg gcggcaaacc atcgggaaag cgcatgtcga cgagcccggc gtcgctgaac
     4921 atttgcacgg cttcgcgcag ggattcgggg cgtagcgtgc cgttcagcgt ggcgagggcg
     4981 gggctcacgc gggcgcccag gcgccgcgcg acgtcgagca ggcgcccgga gcggctgagc
     5041 aaatcggcgt gtgaaaggcc acgctggtcg tggctcaaga gcacgagcgc ggtgagcgcg
     5101 cccggagtca tcgcggtaac gcggttgatc tcgtccatca cccggttgcc gaggcgcgtc
     5161 accagcacgc ggcgcttggc cgggctgagg ctagtcagat cgaggccgag ctcgctcgcc
     5221 acctccgaga gcgagaggat ttggcccacc tggatgttga tgcgaccgta acggtgccga
     5281 agcacatcgc gcgacttcaa gaggccggcc gcgtcttcct tttgcttctc cccgccctgg
     5341 agctcacgtt cgtacgactc gccttcgatg acgcgctcgt agccgatgct aaccggcacg
     5401 aacaaactcg gcggctcggg tacggcgagc gccgcgtcga cgatcatgct caaaatgccg
     5461 aacttgggcg gaatcagctt gccggtgcgg cttcgcccgc cttccaagaa gagctccatc
     5521 ggatagccat ctcggatcag cctgcgcacg taggcgtcca cgaccgcggc gtacagccga
     5581 tccccgcgaa aagagcgacg aatgaagaag ccgccgccgc gccgcagtat ccctcccacg
     5641 gggaaaaagt tgagattgtc gccagccgcg atgagcggca gcggcagatt ctccatgttg
     5701 aagatgtagc tcaggatcag gtagtcgatg tgcgacttgt ggctcggcaa gagcacgagg
     5761 gtcccgcgct tggccgcttc gcggaggcgg tccaaatccg cgctgtcgta ctcgacgccc
     5821 gcgtaaatgc gctggaacaa gcgcttgaag atgacctcca ggaacttgat cgtggtgcgc
     5881 tcgggcgtcg cttgcagctc cgcgagcatc tcgagcgcgc gggcttgcag cgtgtagcgg
     5941 tcggtgtgct cgtcggcgag ctcgtcgatg acggtgcgca ggcgagggct ccgtacgatc
     6001 tcttgccgca cgcgctcggg cggcttttct gcggggcccg tgatgacccg gcgctcgcgc
     6061 tccagacggc gcagcaccgc gtaagtgacg cggcggacga cgacgctgtc gggctgcccc
     6121 gtcgactcgg cgaggaacgc gcgcaggtcg atgggttcgc cgagcttcaa cgtgacgtgt
     6181 ttgtagttgt acagaaactg cgcgaccgta cgcgccgagc tcggccactc acggggcccg
     6241 atgagcacgt cgagcggctc ggtgccgcgc gtatccggaa acttcgacca aacgaacacc
     6301 tgcggcaaaa gcaggatcgg ctcgtctcgt ttgcgctgca gctcgaacaa gactcgaacg
     6361 agctcgtcgc cctccttcaa gccacgaccg ccggttgcgc cggccgcgac gtcgagcacg
     6421 ccgggcgggc gcttcagaaa cagcgccgcc gagcccccgt gggcgagcgc gtcttcgagc
     6481 tcgtcggtgg gcgtgacccg cgagtccggt agaatcgcgt tgagccaacc cttgccgagc
     6541 gggttcagga tccaaagtcc gaggtcgttg acgaagcgaa tccgcggcaa cccgtggcgc
     6601 ttggtcaagt agtcgagggc caagaagtcg atgacattca ggcttctcag cacgtaaacg
     6661 acgcgaccct gctcgcacag ggtcttcact tgctgcgccc aagcgtcatc gacgtggatg
     6721 cgatcgaaaa agcggcggta aagcgcctcc agcaagggat tgggctcgta gatcttcgtg
     6781 agttcgcggc cgctcatggg gtaggttccc gaggccgggg catcatactt gccggatccg
     6841 ccgcccgcga gtactgacgc ctcatgctct cgctttgggg catgaggaca gcttgccgcg
     6901 cgaccgagct cctcgtgctc ggctgtgttc tctccttcgc gctcgctggt tgtcggaaga
     6961 aatctcctgc accgagccac ggac
//
LOCUS       DASTKS010000045         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_6179,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000045 DASTKS010000000
VERSION     DASTKS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6179"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFQ35_00975"
     CDS             complement(<1..399)
                     /locus_tag="VFQ35_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044964987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET9929221.1"
                     /translation="MSLVLTTAWARLLFGTLARAGVSDVVISPGSRSTPFAWAALNTD
                     GLRCHAVIDERSAGFFALGQARVSGRPSVLVCTSGSAAAHYFPAIVEASQAFLPLIVL
                     TADRPFELMDCGAAQAMDQSKLYGGYVRHFF"
     gene            complement(396..1778)
                     /locus_tag="VFQ35_00980"
     CDS             complement(396..1778)
                     /locus_tag="VFQ35_00980"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HET9929222.1"
                     /translation="MTSTASAPRANSAHEQKLREALASARSEQRGERFVSVEVPAPNS
                     DAERLLDLDGASEARFWAEPTGYQFAGLGAVRVLTGAGPSRFREVSVAAQSLFSELGP
                     IASRIRLFGGFAFQPGRAKSPEWRSFGEGRFVLPRIGFERRADGARLSLLFEAAELTD
                     TSLVERTLALAESALEVLSSPVLPESAALEHAALEEREDSQFLELVDSVGAAITRGEF
                     EKIVLARRVALALPRPLSASRVERRLAEIAPECLRFAFRVGSATFLGATPERLVSKVG
                     RAFETEAVAGTVPISEVPAGRLMESTKNRAEQAIVVRELLRAFEPIAAALDHTPTPEI
                     HRLRHIAHLRTKIRGQLREPLHVLDLVERLHPTPAVGGAPTAAALAWIAENEPDERGW
                     YAGPIGWIDGAGDGAFAVALRSGLLERDRERATLYAGAGIVQGSDAQSELAETRWKLK
                     PLLGAFGVAP"
     gene            complement(1775..>2147)
                     /locus_tag="VFQ35_00985"
     CDS             complement(1775..>2147)
                     /locus_tag="VFQ35_00985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9929223.1"
                     /translation="SRTFDGVCIAFGIRNVPDRAKALREMARVTRPGGRIAILELSEP
                     KGGILAPIARFHVHTMVPTIGAWISGAHEYRYLQRSIAAFPPSEVFASLMQESGIDIV
                     NVSPLTFGACHLYVGTPRANT"
BASE COUNT          377 a          722 c          738 g          310 t
ORIGIN      
        1 gaagaagtgg cgaacgtagc caccgtagag cttactctgg tccatggctt gcgccgcgcc
       61 gcagtccatg agctcgaagg ggcgatccgc ggtgagcacg atgagcggca agaacgcctg
      121 cgaagcctcc acgatggccg ggaagtagtg agcggccgcg ctaccggacg tgcacacgag
      181 tacgctcggt cgtccgctga cgcgcgcttg gcccaacgcg aagaaaccgg cgcttcgctc
      241 gtcgatcacc gcgtggcagc ggagcccatc ggtattcagt gccgcccatg cgaacggcgt
      301 cgagcgggaa cccgggctga tgacgacgtc gctgactccg gcccgcgcca aggtgccgaa
      361 caggagccgg gcccaggccg tggtcagcac caagctcacg gagcgacccc gaaagctcct
      421 agcaatggtt tgagcttcca gcgggtctcg gcgagctcgc tctgcgcgtc cgagccttgg
      481 acgatgccgg cgccagcgta gagcgtcgcc cgctcgcggt cgcgctcgag caagccggag
      541 cgcagcgcca ccgcaaaagc tccgtcgccc gcgccgtcga tccacccgat ggggccggcg
      601 taccaacctc gctcgtcggg ttcattctcg gcgatccacg cgagtgcagc cgcggtcggt
      661 gcgccgccga cggcaggcgt tgggtgcaag cgctcgacca agtcgaggac gtgaagcggc
      721 tcgcggagct gcccccggat cttggttcga aggtgcgcga tgtggcgtag ccggtgaatt
      781 tccggagtcg gggtgtgatc cagcgccgcc gcgattggct cgaaggcgcg caacaactcg
      841 cgcacgacga tcgcctgctc cgcgcggttt ttggtgctct ccatcaagcg gcccgccggc
      901 acctcgctga tcggaacggt acccgccacg gcttccgttt cgaaggcgcg accgaccttg
      961 ctcacgagtc gctcgggcgt tgcgccgagg aacgtcgcgc taccgacacg gaatgcgaag
     1021 cgcaggcact caggagcgat ctcggcgagc cgccgctcca cgcgggaagc ggaaagcggc
     1081 cgcggtaggg cgagcgcgac tcggcgcgcg agcacgatct tctcgaactc accccgggtg
     1141 atggccgcgc caaccgagtc gaccaactcg agaaattgcg aatcttcacg ctcttcgagc
     1201 gccgcgtgct cgagcgcggc cgactccggc aaaaccgggg acgaaagcac ctcgagggcc
     1261 gactcggcga gagccaaggt ccgctcgacg agcgacgtgt ccgtgagctc cgctgcctca
     1321 aacaggagag acagccgggc cccgtcggcg cgtcgctcga agccgattcg aggcaaaacg
     1381 aagcggccct caccaaagct ccgccactct gggctcttgg cgcgaccagg ttgaaacgca
     1441 aagccgccga acagccggat gcgcgaggcg atgggcccga gctcgctgaa caggctctgc
     1501 gcggcgacgc tcacctcgcg gaagcgcgac ggtccggcgc ccgtgaggac acgcaccgcg
     1561 ccgagcccgg cgaattggta gccggtgggc tccgcccaaa accgcgcctc gctcgcgccg
     1621 tcgagatcga gcagccgttc ggcgtcgctg ttcggagccg ggacttcgac cgacacgaag
     1681 cgctccccgc gctgttccga acgagccgat gcgagcgctt ccctgagctt ctgctcgtgt
     1741 gccgagttcg ctcgcggggc cgatgccgtc gaagtcatgt gtttgcgcgc ggagtgccga
     1801 cgtagaggtg gcaggcaccg aaggtgaggg gcgacacgtt gacgatgtcg atcccactct
     1861 cttgcatcag ggatgcgaac acctcggacg gcgggaaggc cgcgatcgag cgttgcaagt
     1921 agcggtactc gtgggcaccg gaaatccagg cgccgatggt cggtaccatc gtgtgcacgt
     1981 gaaagcgcgc gatgggcgcg aggatcccgc ctttgggctc cgaaagctcg aggatcgcga
     2041 tgcggccacc cggccgtgtg acgcgcgcca tctcgcgcag cgccttcgcg cgatccggta
     2101 cgttgcgaat gccgaaggca atgcagactc cgtcgaaggt ccgcgac
//
LOCUS       DASTKS010000046         6036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_674695, whole genome shotgun sequence.
ACCESSION   DASTKS010000046 DASTKS010000000
VERSION     DASTKS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674695"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFQ35_00990"
     CDS             complement(<1..680)
                     /locus_tag="VFQ35_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9929224.1"
                     /translation="MAKSAERAPQGFTPDNHPSVLAVRARERAAPAPLTFEAIRDIAV
                     EAGADDAAIVSVDHPDLEEEKAHAQAAFPAARSFVSFVVGTHPENIRSPKRSVANLEF
                     HRAGHLVDEIGHRIAQALSSRGYPTLNPPMAFPMEMSEFPGRTWLIAHKRVAVAAELG
                     KMGLHRSVIHPRLGSFVLLGSVITSAEAERSPPPLSFDPCIGCKLCVAACPVGAIEPD
                     GQFRFSAC"
     gene            complement(718..1275)
                     /locus_tag="VFQ35_00995"
     CDS             complement(718..1275)
                     /locus_tag="VFQ35_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9929225.1"
                     /translation="MRYPEGHKEAVHEAIVRGAAKALREKGISGIGIPALMKAAGLTH
                     GGFYSHFRDRDALVGEAIRAAAEETSQGVFAEAKSVTEMLAHYLSLGHVEHPEMGCVV
                     AALAAESPRQSKAVRRVFGEVVRGLIALVEKKLNPRPGRTPSEAALRLTATMVGAVVL
                     ARSVDDAQLADRILKAARHTPELRE"
     gene            1456..3033
                     /locus_tag="VFQ35_01000"
     CDS             1456..3033
                     /locus_tag="VFQ35_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HET9929226.1"
                     /translation="MSTFRDALEAASRGTHENGRRWLFVPYDQLTDRLGPLSREPATS
                     LGIVLVESPAKAARRPYHKQKLALVMANLRTFALEQASRGVAVRHVVAHGGYAAALSP
                     LVAKLGPLRMMEPAERELRQELAPLVGSGALETLPHEGWLTTDADFEAASSDKQPPWR
                     MDRFYAAVRRRTGVLMKGGKPLGGRMSFDTENRERWRGEPPAPMPPRFEPSAISREVG
                     DLVQSRFADHPGTLDLSSLPTTHADAQSSLDWAMANCLPHFGPYEDAMSTRSTGLFHT
                     RLSPLINLHRLTPREVLARVLSVPIPLASQEGFVRQLLGWREFVRHVHEKTDGLRLRP
                     NGSGVEPLTPRRGSTLPDVFWGRASGLACLDRVVESVWSEGYSHHITRLMILSNIATL
                     LDVSPRELTDWFWVAYIDAYDWVVEPNVLGMGTYAVGELMTTKPYVAGSAYIHRMSDF
                     CGTCDFDPKRTCPLTPLYWSYLARNETELASNSRMLVPLAALRKRPESQRVRDERVFV
                     TVRDALARGEKLGPDVR"
     gene            3222..4328
                     /locus_tag="VFQ35_01005"
     CDS             3222..4328
                     /locus_tag="VFQ35_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HET9929227.1"
                     /translation="MTDKTLFDPVRVGRLTLANRLVMNPMTRCRADAEGRVGELTATY
                     YTQRSSAGLIVTEGIAPSPNGKGYARIPGLWSQDQVESWKPVTQAVHAAGGHIFAQVM
                     HTGRVSHPANFTAGAKVVAPSALAFEGQQIWTDGQGMQSYPVPEALDERGIQQAKDEF
                     QNAAKNAIAAGFDGVELHGANGYLLEQFLSPVTNQRTDGYGGSVANRIRFVVEVAQAV
                     AAAIGGDRLALRISPYGKNGGMSEYPEIDETYLALAKALAPLGLAYLHVADHFATSNG
                     GPGAHPGLEALKSKLREALPGSAFLVGGSLDKDAAERAVKEGKLDLVGFGRAFLANPD
                     LVRRFREGLELNAADPSTFFTPGVKGYTDYATAS"
     gene            complement(4391..4918)
                     /locus_tag="VFQ35_01010"
     CDS             complement(4391..4918)
                     /locus_tag="VFQ35_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9929228.1"
                     /translation="MLSPDERRVRYAEVKRRLESLLADENDWIAALATVACELHHAFD
                     YFDWTGFYRAVSSDLLVIGPYQGSHGCLRIPFSRGVCGAAARTRTTQRLDDVELFPGH
                     IACSSSTRSEIVVPVISPRGVLAVLDVDSDAEAAFDATDQLELESLCADLARRFPNGE
                     LPSVVNAASLGSANG"
     gene            5003..5782
                     /locus_tag="VFQ35_01015"
     CDS             5003..5782
                     /locus_tag="VFQ35_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9929229.1"
                     /translation="MTKRRTYQVKEVARLTGVSVRALHHYDEIGLLVPSSHTDAGYRL
                     YDDRDLLRLQQILIGRELGLPLEEIKRSLDDPHFDRQAALRKQRRELEKSAARAAAML
                     RAVDAALAAFATEGERTMTDMSKDEITALFEGFDPSQYESEVNERWGTTDAYEESARR
                     TSKYTQADWERYKAEADAILNHAARLFRSGVSPREAEAGAVAERHRLSIDRWFYPCSP
                     GMHAGLADLYEADARFTQNIDKYTPGLTAWWVAAIRENSSR"
     gene            5819..>6036
                     /locus_tag="VFQ35_01020"
     CDS             5819..>6036
                     /locus_tag="VFQ35_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929230.1"
                     /translation="MELGWISLLSLAALSLCTCRADPNSRRDPAGNVGSSTATNTDAV
                     WFSFRAPPTEAQGRSDATRRTQAFVVYA"
BASE COUNT         1143 a         1971 c         1897 g         1025 t
ORIGIN      
        1 tagcaagcgg aaaagcgaaa ctgaccgtcg ggttcgatgg cgcctactgg acatgccgcc
       61 acacacagct tgcagccgat gcaagggtcg aaggaaagtg gaggcgggga acgttcggcc
      121 tccgctgacg tgatcacgct accgagcagc acgaacgaac ccaatcgcgg gtggatcacg
      181 ctgcgatgca ggcccatctt cccgagctcg gccgcaactg cgacgcgttt gtgggcaatg
      241 agccacgtgc gccccggaaa ctcgctcatc tccatcggaa aggccatcgg cgggttcagc
      301 gtgggatagc ctcgcgaaga cagcgcctgc gcgatgcggt gcccgatctc gtccacgaga
      361 tgtccggccc ggtgaaattc gaggtttgcg acggaccgct tggggctgcg aatgttttcc
      421 gggtgtgtgc ccactacgaa agacacgaac gaacgcgctg cggggaacgc cgcctgcgcg
      481 tgcgccttct cttcctccag atcggggtgg tcgacgctca cgatggccgc atcgtcggcg
      541 cccgcctcca cggcgatgtc gcggatcgct tcgaaagtga gtggagccgg cgcggctctc
      601 tctcgagcgc gaaccgccag caccgacgga tgattgtccg gagtaaaacc ttgcggagct
      661 cgttcagcgc tttttgccat gatgaccatc atttcatcga acaaaaaaaa gattcgttca
      721 ctctcgaagt tctggggtgt ggcgcgccgc tttcaaaatc ctgtccgcga gctgcgcgtc
      781 gtccacggac cgcgcgagaa ccaccgcccc gaccatcgtc gcggtcaagc gcagggcagc
      841 ctcactgggc gtgcgccctg gccgcgggtt gagcttcttc tcgacgagcg cgatgagccc
      901 tcgcaccacc tccccgaaga cgcgtcgcac cgccttcgac tgccgcggac tttcggctgc
      961 gagcgcggca acgacgcagc ccatttccgg gtgctcgacg tgaccgaggg aaaggtaatg
     1021 cgcaagcatc tccgtcacgg atttcgcctc ggcaaagaca ccttgggaag tctcctcggc
     1081 ggcggctcga attgcctccc ccacgagcgc gtctcgatcc ctgaagtgcg agtagaaccc
     1141 cccatgcgtg aggccggctg ctttcatcag cgctggtatg ccgatccccg agatgccctt
     1201 ctcacgaagt gccttcgccg ccccgcgcac gatcgcctcg tgcacggctt ctttgtggcc
     1261 ttcggggtat cgcattatgc tcatcatgtc accgctcgcg acgaacggca agcgcggagc
     1321 gcacccgccg tcgaatcgct ggcgcaacac aaggccccga cgccgacccg agcgtcgcca
     1381 aagagcacac gcgggacgct cgtggtattg acgtcattcg agcatgcgag accgctcagc
     1441 aaaggagcga acgcgatgag cacctttcga gacgcgctcg aggccgcgag ccgtgggacg
     1501 cacgagaatg gtcgccgctg gttgttcgtc ccctacgatc aactcaccga tcgcctcggt
     1561 ccgctgtcga gagagccagc cacctcgctc ggcatcgtcc tcgtcgagag tcccgccaag
     1621 gcggcacgtc ggccttacca caagcaaaag ctcgcgctgg tgatggcaaa cctgcgcact
     1681 ttcgcgctcg aacaagcttc ccggggcgtc gcggtgcgcc acgtggttgc gcacgggggc
     1741 tacgctgcgg cgctctcgcc gctcgtagcc aagctcgggc cgcttcgcat gatggaaccc
     1801 gcagagcgcg agttacggca agagctcgcg ccgctcgtcg gaagcggagc actcgaaacc
     1861 ttgccacacg aagggtggct gacgaccgac gccgacttcg aggctgcaag ctccgacaag
     1921 cagccgccgt ggcgcatgga ccgcttctat gcggcggtgc gcaggcgaac gggcgtgctc
     1981 atgaagggcg gaaagccgct cggtgggcgt atgagcttcg acaccgaaaa ccgtgaacgc
     2041 tggcgcggcg aaccgcccgc tccgatgccg ccgcggttcg agccgagcgc aatcagccgc
     2101 gaggtcggcg acctcgttca aagccgcttc gccgaccatc ccggcacgct cgacttgagc
     2161 tcactgccaa ccacgcacgc cgacgcgcag agctcgctcg actgggcgat ggccaattgc
     2221 cttccccact tcggcccgta cgaggacgcc atgtcgacgc gctctaccgg cctatttcac
     2281 acccggctct cgccgctcat caaccttcat cggctcacgc cgcgcgaggt gctagctcgc
     2341 gtgctttcgg tgcccatccc gctcgcgagt caagagggct tcgtgcggca gctcctcggt
     2401 tggcgcgagt tcgtgcgtca tgttcacgaa aagacagacg gcttgcgcct gcggccgaat
     2461 ggctccggcg tggagccgct caccccgcgt cggggctcga ctttacctga cgtgttctgg
     2521 ggtcgggcgt cggggttggc ttgcctcgat cgcgtcgtcg agagcgtttg gagcgaggga
     2581 tacagccatc acatcactcg attgatgatc ctctccaaca tcgcgacgct gctcgacgtc
     2641 tcgccacgtg aactcaccga ttggttctgg gtggcgtaca tcgacgcata cgactgggtc
     2701 gtcgagccca acgtgctcgg catgggcacc tacgccgttg gcgaattgat gacgacgaag
     2761 ccctatgtag ccggttctgc ctacattcat cgtatgagcg acttttgtgg aacctgcgac
     2821 ttcgacccga agcgcacttg tccgttgacg ccgctctact ggagttacct cgcgcgaaac
     2881 gagacagagc tcgcgagcaa ctcacgaatg ctcgtgccgc tcgctgccct ccgaaaacgg
     2941 ccagagtccc agcgcgttcg cgacgaaagg gtatttgtca cggttcgcga cgcactcgcg
     3001 cgaggagaaa agctgggccc agacgtccgc taagagccag tcctaaatcc tggacgggag
     3061 ggatttggga ttggctctta agggccccgc aaatgcatga gtcagtcgcg aacgcccgcc
     3121 ccggcgaggc cggaaactgg aggcgttgga ccttcaacca atgcctttgg acaattcggc
     3181 atggatcgtc caacgctcgt cctgctagtg agctatccac cgtgacagac aagactcttt
     3241 tcgaccccgt tcgcgtcggc aggctcaccc tcgccaatcg tctcgtgatg aatccgatga
     3301 cccgctgccg ggccgacgcg gaaggtcgtg tcggcgagct caccgcgacc tactacaccc
     3361 agcgttcgag cgcgggcctc atcgtcaccg agggcattgc tccttcaccc aacggcaaag
     3421 gctacgctcg cattccgggt ctatggagcc aagaccaggt agagagctgg aagccggtca
     3481 cccaagccgt gcacgcggcg ggcgggcaca tcttcgcgca ggtcatgcac accggtaggg
     3541 tgagccaccc ggcaaacttc acggctggag ccaaggtcgt agcgccgagc gccctcgcgt
     3601 tcgaaggcca gcaaatttgg actgacgggc agggcatgca gtcctaccca gttccggagg
     3661 cgctcgacga acggggcatc cagcaggcca aggacgagtt ccagaacgcg gccaagaacg
     3721 ccatcgcggc cggattcgat ggggtcgagc tccacggcgc gaatggctat ctgctcgaac
     3781 agttcctgag tccggtcacg aaccaacgaa ccgatggcta cggtggctcg gtcgcgaatc
     3841 gaatccgttt cgtcgtggaa gttgcgcagg ccgtcgcggc cgctatcggc ggcgatcggc
     3901 tcgctctccg catttcgcca tacgggaaga acggcgggat gagtgagtac ccagagatcg
     3961 acgagacgta cctcgcgctc gccaaggccc tggctccgct cggcctcgcg tacttgcacg
     4021 tcgcagatca cttcgcgacc agcaacggcg gccccggtgc gcatccgggg ctcgaggcac
     4081 tgaagtccaa gttgcgcgag gccctaccgg gaagcgcatt tttggtgggc gggagcttgg
     4141 acaaggacgc ggctgaacgc gcggtgaagg agggcaagct cgatctagtc gggttcggaa
     4201 gagccttcct cgccaacccc gatctagtga gaaggttccg tgaagggctc gagctcaacg
     4261 cggcagatcc gagcactttc ttcacgccgg gcgtgaaggg ctacaccgac tacgcgaccg
     4321 cgagttgaag cgtcgccctt cctcgagcag agagccaatc cctgatccct ccagggtcta
     4381 ggactggctc tcatccattg gcggagccta gactcgcggc gttgaccacg gacggaagct
     4441 ccccgttggg gaagcgccgc gccaagtccg cgcacaacga ctcgagctcc agttgatccg
     4501 tcgcgtcgaa ggcggcttcc gcgtccgaat ccacgtcgag cactgcgagc acgccgcgtg
     4561 gcgaaatcac cgggacgacg atttcggagc gggtcgagct cgagcacgcg atgtgacccg
     4621 gaaagagctc gacatcgtcg aggcgttgag tggtccgggt ccgcgccgca gcgccacaca
     4681 caccgcgcga gaacggaatc cgaaggcaac cgtgcgagcc ctggtagggg ccgatcacga
     4741 gcaggtcgct cgataccgct cggtagaacc cggtccaatc gaaatagtcg aaggcgtgat
     4801 gtagctcaca cgccacggtc gcgagcgcgg cgatccagtc gttctcgtcg gccaggagag
     4861 attcgaggcg tcgtttgact tcggcgtagc gaacgcggcg ctcgtcgggg ctcagcatgg
     4921 gcgggcattt tagcccgttc gcggaaattg gcgatgcaat cggcttgacc ctcacgcagc
     4981 gtgaggccgt aggttcatcc caatgacgaa gcgccgcacg taccaggtca aggaggtcgc
     5041 tcgcctgact ggcgtcagcg tgcgcgcact ccaccactac gatgagatcg ggctactcgt
     5101 accgtcgagt cacacggacg cgggttaccg cttatacgac gaccgcgacc tgctgcggct
     5161 tcagcagatc ctcattggaa gggagcttgg ccttccgctc gaagagatca agcgatcgct
     5221 cgacgacccg cacttcgatc ggcaggcagc ccttcgaaag caacgtcgcg agctcgagaa
     5281 gagcgcagcg cgcgcggccg ccatgctgcg cgccgtcgac gccgcgctcg ccgccttcgc
     5341 gaccgaagga gaaagaacga tgaccgacat gagcaaggac gagatcaccg cgctgttcga
     5401 aggcttcgat ccatcgcagt acgagagcga agtcaacgag cgttggggca cgacggatgc
     5461 ctacgaggag tccgcgcgtc ggactagcaa gtatacgcaa gcggattggg aacggtacaa
     5521 ggccgaggcc gacgcgatcc tgaaccacgc tgcacggctg ttccgctcgg gggtttcacc
     5581 gcgcgaagcg gaagcggggg cggtcgcaga gcgtcatcgg ctctccatcg accgctggtt
     5641 ttacccgtgc tcaccgggaa tgcatgccgg cctcgcggac ctctacgagg ccgatgcgcg
     5701 cttcacccag aacatcgaca agtatacgcc cgggctcacc gcctggtggg tcgcggccat
     5761 tcgggaaaac tcgagccgtt gacgcacttt cgttctcgcg catgtcacag ttcgagaagt
     5821 ggaactcggc tggatctcgc tcctgagcct tgcggctctc tctctgtgca cgtgccgagc
     5881 agacccgaac tcgcgacgag atcccgcggg gaacgtcgga tcgagcaccg caaccaacac
     5941 ggatgcagtc tggttctcgt ttcgggcgcc gccaaccgag gcccaaggcc gctccgatgc
     6001 gactcgccgc acccaggcat tcgtggtcta cgcgag
//
LOCUS       DASTKS010000047         6710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1789629, whole genome shotgun sequence.
ACCESSION   DASTKS010000047 DASTKS010000000
VERSION     DASTKS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789629"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFQ35_01025"
     CDS             <1..855
                     /locus_tag="VFQ35_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9929231.1"
                     /translation="EYCTDVDYDCLKSWYDESNKKTVCEEFKPESRCVGKRVKKRFCI
                     DMYTWPNEKGARPEVMNRFHQAQLKCAAIGKRLCTESEWTMACEGPRLLPFPYGYVRD
                     SNKCLGDVEWDSPDMKKVAKRDPEELARLWKGVRNGSQPGCISEYGVADLPGNVDEVV
                     SSETFDGTWKAKYDSVHTGGPWYKGVRNQCRPKIYTHDEGFYYYFLGFRCCSEPDGKP
                     TDFRTARQIKENWKLSRVEGIAGMKLDEIKEKLLQKKKGECKCGEKDTRCKTICGTLL
                     GPNAKDYR"
     gene            complement(877..1338)
                     /locus_tag="VFQ35_01030"
     CDS             complement(877..1338)
                     /locus_tag="VFQ35_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET9929232.1"
                     /translation="MQKASPLARWLATWFGCGASPIAPGTVGSLGTLPLAYVLVGAGA
                     YAYWGVTLALTLLGTWAAERTAQALGDDDPSSVVIDEVVGTLLALGLAGPQISASIAA
                     FVLFRVFDITKPGPIDTVQHLRPAGLGIMADDVLAGLAAGALVRLGTLFFG"
     gene            complement(1350..2069)
                     /locus_tag="VFQ35_01035"
     CDS             complement(1350..2069)
                     /locus_tag="VFQ35_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929233.1"
                     /translation="MVTADGNEHEHDNERDDDDDDDVNVESSRPAKPKASKRPARPPE
                     EEEGVIGGRFLTLGIIMLVAVVIPPSQLRELLEPKEPESTHVEQWAPGVESTVRITLV
                     TADYGLLGCAAEQSFDGVHCAYKSQTEIWPRDPNEPNDDNNNKLNIVQPYRTWYDNKL
                     VFVAGLWATPNVAFRLHQEPSAGILPDKLARFAVECKVKFVGRFDSVKLRWNPQQSWV
                     DPDGPAWVGKALTCKLIDEPQ"
     gene            complement(2166..2990)
                     /locus_tag="VFQ35_01040"
     CDS             complement(2166..2990)
                     /locus_tag="VFQ35_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9929234.1"
                     /translation="MSTGTSYVFFALAVLVACRPEARAEPSATATASIPSPSSAPAPA
                     EAPAVLETAASSPPPPVAAATPSASATPVSRKAHRVVVLGDSLSDPRVHGGHYLDGLG
                     ACPGITIDNFAKGGFMVNQMRRRFESEVTPTLTKDHTDLIVFGGVNDLYSDETAGRTV
                     EKISADLTRIYDGAKAHGLRVIAITVAPWGGFTRYYNGRRGQATERLNAWIRQQQAEH
                     RVDVVVDAAPLLACGDVERLCPEYEPPFHDGLHFGRLGHEKLSRALLDAAFAACRP"
     gene            2989..3693
                     /locus_tag="VFQ35_01045"
     CDS             2989..3693
                     /locus_tag="VFQ35_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9929235.1"
                     /translation="MSFSEVYRKTRKVPDIAFNMYVCRPVAAVVVDWVKDTRVTPNQI
                     TLSAVLVSAVASACIALVPGYLGLLVGVLVFEASYVLDCADGMLARWRGVSSPEGHLL
                     DFLMDELKAFVLLAVVAYRLFAERNDERFLLLGLAGLVALASGIALTTFVRRPEIQGP
                     ERAPAKSPRGGALGLLEGGAKFVVHYPSYIWLAAALGRIEIYFFPYIFINALYAARMF
                     LCVSVRFGRFAPRVTR"
     gene            complement(3683..5245)
                     /locus_tag="VFQ35_01050"
     CDS             complement(3683..5245)
                     /locus_tag="VFQ35_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929236.1"
                     /translation="MTELAPPGYASRERGDGPDLAFPVGVSAFAAATLLTSFYEVCVA
                     APRAFARLSQPNWSLSIALGAIALGAWLTRRSRALGADTGFALLACFSAGSAHALALG
                     FAHGPFLDIFEVAVPLYGGLLFGATGLALARDVGRELAALEAVPYLMNPLRLAALSAG
                     WLGVLLTFPALGILRRGALVAALLTGAALLLPFVERLFGKTRRKEPRSLQALATFATV
                     ASLGVAHHSVPLSVVQSHPGDVVYALETGRGEHVIARSQGGLLLFSNDVLALTTNDST
                     RFAEALVHPALSLAARRARVLAIDDGTGPVAREALRWKDLESLTLVPHDPALAQLARE
                     GDWFSALTSEALNDRRVTWVDREAAPFVLESKELFDVVLLNASDPSDYRSGKYFSRYW
                     LEAVRMHLGADGVLAVQTTSPLRTPRAFASILETLNRAGFSVVSYRAALPTLGEWSFA
                     LAFTTSEPIASRLAHVHSIPTGTEYVSDRMLPTLLASSPEPPPHDTAPVNHLWDQPVV
                     ELYRREDRSLTE"
     gene            complement(5242..6645)
                     /locus_tag="VFQ35_01055"
     CDS             complement(5242..6645)
                     /locus_tag="VFQ35_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HET9929237.1"
                     /translation="MGSSISKVCEFCRHTVLRSDRGLSELGKVADLAETPSLIAVADE
                     GTLAGRPFRVMGRVQLDHGKGPWDEYYVAFDYGQSWGWLAYAQGRWFVTSPVSGIGVL
                     PFDALRPELELTLGAAGNFRVSEVRTGRIVSAEGELPGAFPAGFQRAYADLSGANNAF
                     ATLDYGDGSTPPVAFLGWEFPESALSVTQLGPRSANKVKVTALKCPNCGGDVPALLPG
                     RSERLGCPYCGAVSDLAERKIIAQQEKARAMPIIPIGTRGTLGGVEYTCIAALRRGSR
                     FDGELYEWEEFLLWSQSEGFRWLVLDPETGWLFVTPISMGELDLRESPAFVEFRGRRF
                     VQRNDNSAEVRYVLGEVYWKCEVGETVQVTDFVSGDDVVSREALPTEVNWSFCQKQSW
                     PDIAQAFGLPLAAHARRVAGSAKSSSGIGAGTLIVIVLIVLVLIILMASAPAGTSSVS
                     GGSVGSYRGGGVFSGGK"
BASE COUNT         1202 a         2143 c         2265 g         1100 t
ORIGIN      
        1 gagtactgca ccgacgtcga ctacgactgc ctgaagagct ggtacgacga gtcgaacaag
       61 aagacggttt gcgaggagtt caagcccgaa tcgcgctgcg tcggcaagcg ggtcaagaag
      121 cgattttgca tcgacatgta cacctggccg aacgaaaagg gcgcgcgccc cgaggtgatg
      181 aaccgctttc atcaggccca gctcaaatgc gccgcgatcg gcaagcgact ctgcacggag
      241 tccgagtgga cgatggcttg cgaggggccg cgcttgttac cgtttcccta cggttacgtg
      301 cgagacagca acaagtgcct cggcgacgta gagtgggatt cgcccgacat gaagaaggtg
      361 gccaagcgcg acccggaaga gctcgcgcgg ctttggaagg gcgttcgtaa cggaagtcag
      421 cccggctgca tcagcgagta cggcgtcgcg gatctacctg gcaacgtcga cgaggtcgtc
      481 tcgagcgaaa ccttcgacgg gacctggaag gcaaaatacg acagcgtgca caccggtgga
      541 ccttggtaca agggcgtgcg caaccagtgt cgccccaaga tttacacgca cgacgagggc
      601 ttctattact acttcctcgg ctttcgctgc tgctccgagc ccgacggcaa gccgactgat
      661 ttccgcaccg cgcgccaaat caaagagaat tggaagctct ctcgcgtcga gggcatcgcc
      721 ggcatgaagc tcgacgaaat caaagagaag cttcttcaga agaagaaggg cgagtgcaag
      781 tgcggcgaga aggacacccg ctgcaagacg atctgcggca cgttgctcgg cccaaacgcc
      841 aaggactatc gctgaggggc cgagcgcttg cgggccttag ccgaaaaata gggtaccgag
      901 ccgcacgagc gctcccgccg cgagaccggc caacacgtcg tccgccatga tgccgagacc
      961 agccgggcgg aggtgttgaa cggtgtcgat ggggccaggc ttggtgatgt cgaacacgcg
     1021 aaacagcacg aacgcggcaa tcgaagcgct gatttgaggg cctgcgagcc cgagcgcgag
     1081 cagcgtgccc acgacctcgt cgatcacgac cgagctcgga tcgtcgtcgc cgagcgcttg
     1141 cgccgtacgc tcagccgccc acgtcccgag cagcgttagc gcgagcgtga cgccccaata
     1201 cgcgtaggct ccggcgccga cgagcacgta ggcgagcgga agcgtgccga gcgaaccgac
     1261 cgtgccaggc gcgatcggcg atgcgccgca cccgaaccat gtggcgagcc agcgtgcgag
     1321 cggagacgct ttctgcacga cctcgtcttc tattgcggtt cgtcgatgag cttgcaggtg
     1381 agggcttttc cgacccaggc tgggccgtcc ggatcgaccc agctttgctg cgggttccag
     1441 cgcagtttga cgctgtcgaa tcgacccacg aacttcacct tacactcgac cgcgaagcgc
     1501 gcgagcttgt cgggaaggat ccccgccgac ggctcctgat gaaggcggaa agccacgttc
     1561 ggcgtcgccc aaaggccagc gacgaacacg agcttgttgt cgtaccaagt gcggtacggc
     1621 tgaacgatgt tcaacttgtt gttgttgtcg tcgttaggct cgttcggatc gcgcggccaa
     1681 atctcggttt gcgacttgta ggcgcagtgc acgccgtcga agctctgctc ggccgcacaa
     1741 ccgagcaagc cgtagtctgc cgtgaccaac gtgatccgca ccgtcgattc gaccccgggc
     1801 gcccactgct cgacgtgcgt cgactcgggc tctttgggct cgagcaactc gcggagctgc
     1861 gaaggcggga tcacgacggc cacgagcatg atgatgccca gcgtgaggaa gcggccgccg
     1921 atgacgccct cctcctcctc gggggggcgt gccgggcgct tgctcgcctt gggcttggcc
     1981 gggcgactcg attcgacatt gacgtcgtcg tcgtcgtcat cgtcgcgctc gttgtcgtgc
     2041 tcgtgctcgt tcccgtctgc cgtcaccatg ctgcgccccc aaatagcgca aattggtacg
     2101 ctggcgcagc ctgtcagttg aaccccgcgt tttttcgggg aatgtgcgca gaattatcgc
     2161 tgggctcagg gccgacaggc cgcgaacgcc gcgtcgagca gcgcccggga gagcttttcg
     2221 tgaccgagcc ggccgaagtg caggccgtcg tggaacggcg gctcgtactc ggggcagagc
     2281 cgctcgacgt ctccgcaggc gaggagcggc gccgcgtcga cgaccacgtc gacgcgatgt
     2341 tccgcctgct gctgccggat ccaggcgttg aggcgctcgg tggcttggcc gcgccgcccg
     2401 ttgtagtagc gcgtgaagcc gccccacggc gcgacggtga tggcgatgac gcgcaacccg
     2461 tgggccttcg ctccgtcgta gatgcgggtc aggtcggcgc tgatcttctc gaccgtgcgt
     2521 ccggcggtct cgtcgctgta gaggtcgttc acgcccccga agacgatgag atccgtatgg
     2581 tccttcgtga gggtcggtgt gacctcgctc tcgaagcgac ggcgcatttg gttcaccatg
     2641 aatccgccct tcgcgaagtt gtcgatggtg ataccggggc aggccccgag cccatcgaga
     2701 taatgaccgc cgtgcacgcg agggtcgctc agcgagtcgc cgaggacgac gacccggtgc
     2761 gccttccgag agacgggcgt cgcactggca ctcggcgtcg ccgctgcgac aggaggcggc
     2821 ggagacgaag ccgcggtttc gagaacggcg ggagcttcgg ccggcgcggg agcagacgat
     2881 gggctaggta tgctcgcggt ggcggtggcg ctcggctccg cgcgcgcctc gggccggcaa
     2941 gctaccaaga ccgctagcgc gaagaacacg taagatgtcc cggtcgacat gagcttctcc
     3001 gaagtttaca ggaagacgcg caaggttccc gatatcgcgt tcaacatgta cgtttgtcgt
     3061 ccggtggcgg cggtggtcgt cgactgggtg aaagacactc gcgtaacacc gaaccagatc
     3121 acgctttcgg cggtgctggt gagcgcagtg gcttcggcgt gcatcgcgct cgttccagga
     3181 tacttgggcc tgctcgtcgg cgtgctcgtg ttcgaggctt cgtacgtcct cgactgtgcc
     3241 gatggcatgc tggcgcgctg gcgcggggtt tcctcgccgg aagggcatct gctcgatttt
     3301 ttgatggacg agctcaaagc cttcgtgctc ctggcggtgg tcgcttaccg cctcttcgcg
     3361 gagcgaaacg acgagcggtt tttgctgctt ggtttggcgg ggctcgtggc gctcgcgagc
     3421 gggattgctc tcaccacctt cgtgcggcga ccggagattc aaggtccgga gcgcgcgccg
     3481 gcgaagtcgc cgcgtggggg cgcgctcggc ctgctcgagg ggggcgcgaa gttcgtcgtg
     3541 cactacccgt cgtacatttg gctcgcggcc gcgctcggac gcatcgagat ctactttttc
     3601 ccgtacatct tcatcaacgc cctttatgca gcgcgcatgt ttctttgcgt cagcgtgcgc
     3661 ttcgggcgct tcgcgcctcg ggtcactcgg tgagggagcg gtcctcgcga cgatagagct
     3721 cgacgacggg ctggtcccaa aggtggttca cgggcgcggt gtcgtgaggc ggcggttcgg
     3781 gagacgacgc cagcagagtg ggaagcatgc ggtcgctcac gtactcggtc ccggtcggaa
     3841 tcgagtggac atgtgccagg cgactagcga tcggctcgga ggtagtgaag gcgagagcaa
     3901 agctccactc accgagcgtc ggaagcgcgg cgcggtagct caccaccgaa aacccagcgc
     3961 gattcagcgt ctcgaggatg ctcgcgaaag cacggggggt acgcagtggc gacgtggttt
     4021 gaaccgcgag cacgccgtcg gcgccgaggt gcattctcac ggcttcgagc cagtaacggc
     4081 tgaaatactt cccgctgcgg tagtcgctcg ggtcgctcgc gttcaggagc accacgtcga
     4141 agagctcctt gctttcgagt acgaaggggg cggcttcacg atccacccag gtgacgcgcc
     4201 ggtcgttcag cgcctcgctc gtaagagccg agaaccagtc cccttcgcgc gcgagctgcg
     4261 cgagagcggg gtcatgcgga acgagcgtca gggactcgag atctttccag cgcagcgcct
     4321 cgcgagcgac gggcccggtt ccgtcgtcga tggcgagtac ccgcgcacgt cgagccgcga
     4381 gcgagagcgc cggatgcacg agcgcctctg cgaagcgcgt cgagtcgttg gtcgtgagtg
     4441 cgagcacgtc gttcgaaaag aggagtaagc cgccttgcga gcgcgcgatg acgtgctctc
     4501 cgcgccccgt ctcgagggcg tacacgacgt cgcccggatg gctctgaacg accgatagcg
     4561 gcacggagtg gtgcgccacg ccgagtgacg ccaccgtcgc gaacgtcgcg agcgcctgca
     4621 agctccgcgg ctccttcctg cgcgtcttgc cgaagagtcg ctcgacgaac ggcagcaaga
     4681 gcgcggctcc cgtgagcagc gcggcgacca acgccccgcg ccggagaatg ccgagggcag
     4741 gaaaagtcag caaaacaccg agccagcctg cgctcaaggc cgcgagccgt aggggattca
     4801 tcaggtacgg gaccgcctcg agcgcggcga gctcacgccc gacgtcccgc gccagcgcga
     4861 gcccggtggc tccaaagagg agccctccgt agagcggcac cgcgacttcg aaaatatcga
     4921 gaaagggccc gtgcgcgaac ccgagcgcga gcgcgtgcgc actgcccgcg ctgaagcatg
     4981 cgagcaatgc gaagcccgtg tctgccccga gcgcacgtga acgccgggtg agccaggccc
     5041 cgagagcgat cgcgccgagc gcgatggaaa ggctccagtt tggctgagag agccgcgcga
     5101 acgcgcgcgg agccgcgacg cagacctcgt agaacgacgt gagcagagtt gccgccgcga
     5161 acgccgagac gccaacgggg aacgcgaggt ccgggccgtc accgcgctcg cgagatgcgt
     5221 agccgggcgg cgcaagctcg gtcacttgcc gcccgagaag acgccgccgc cgcggtagct
     5281 cccgacactg ccaccgctca cggaagacgt gcccgcgggc gcgctcgcca tcaagatgat
     5341 gagcacgagc acgatcagca cgatgacgat cagcgtgccc gcgccaatac cgctgctcga
     5401 tttcgcgctc ccggcgacgc ggcgagcgtg cgccgcgagc ggtagaccga acgcctgcgc
     5461 gatgtcgggc cagctctgct tctgacagaa gctccagttt acttcggtgg gcaaggcttc
     5521 gcgggacacg acgtcgtcgc cactcacgaa gtcggtgact tgcacggtct cgcccacttc
     5581 gcacttccag tacacttcgc cgagcacgta gcgcacctcg gcggagttgt cgtttcgctg
     5641 cacgaaacgg cgcccacgaa actctacgaa cgccgggctc tcacgcaggt cgagctcgcc
     5701 catcgagatg ggggtgacga agagccatcc cgtttccgga tccaaaacga gccagcggaa
     5761 cccctcgctc tgactccaca gcaggaactc ttcccattcg tagagctcac cgtcgaagcg
     5821 gctaccgcgg cggagcgcgg cgatgcaggt gtattcgact ccgccgaggg tcccacgcgt
     5881 gccgatcggg atgatcggca tggcccgcgc cttctcttgt tgagcgatga tcttgcgttc
     5941 ggcgagatcg ctcacggcgc cgcagtaagg gcacccgagt cgttcgctgc gacccggcaa
     6001 gagcgccggc acgtcgccgc cgcaattcgg gcacttgagc gcggtgactt tgaccttgtt
     6061 cgcgctccgc ggcccgagct gcgtgacgct gagcgcgctc tctgggaact cccagcccaa
     6121 aaaggcgacc ggcggcgtcg acccgtcgcc gtagtcgagg gtcgcgaaag cgttgtttgc
     6181 gccgctgaga tccgcgtagg cgcgctggaa cccggcggga aaagctcccg gtagttcccc
     6241 ctccgccgag acgatgcggc cggtgcgcac ctcgctgacc cgaaagttgc ccgccgcgcc
     6301 gagtgtcaat tcgagctcag ggcggagcgc atcgaagggc agaacgccga tgccggaaac
     6361 cggagaggtg acgaaccagc gcccctgcgc gtaggcaagc cagccccagc tctgcccgta
     6421 gtcgaaggcg acgtagtact cgtcccaagg ccctttgccg tggtcgagct gcacacgacc
     6481 catcacgcga aaggggcggc ccgcgagcgt gccttcgtcg gcgacggcga tgagcgaggg
     6541 cgtctccgcc aagtcggcga ccttgccgag ctcgctgagc ccgcgatcgc tgcgcagcac
     6601 ggtgtgccgg cagaactcgc aaaccttgga gatcgacgag cccaccgaaa attcgatggg
     6661 cgcaccgcaa ctcgggcaat ttccttgagc ggcagccatt caggtgggcc
//
LOCUS       DASTKS010000048         5307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1344589, whole genome shotgun sequence.
ACCESSION   DASTKS010000048 DASTKS010000000
VERSION     DASTKS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344589"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFQ35_01060"
     CDS             complement(<1..1002)
                     /locus_tag="VFQ35_01060"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HET9929238.1"
                     /translation="MGEAIRRLPKASGALVFVSGELVASFEEVSSHVAEALGRVPALV
                     AAGHGVLTERGEIEGESALAAVVWEGGTATPISIDDTGGPDLGLSTAEALEAHAGSSS
                     TAFLFLRPRGVLPHMLDPLHSLRFGALVGAGTTGDGGVALVSSGKARVGNIGALVLKG
                     GTPPSVRPSPACRLLTPLIPITATRGPMVLELGGEKALEALSLATANLKDQPLLFVAL
                     AQKPTDESDVPPMLLRGIQGIDPVRQGIVVSEEVREGLLMAFAVRDPVAARSDLELAT
                     ARLARETGGAAARFGVYLSCAGRGTSLYGSPDVDTRVIRARFGDLPLAGMHSSFEIAP
                     "
     gene            1215..2024
                     /locus_tag="VFQ35_01065"
     CDS             1215..2024
                     /locus_tag="VFQ35_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9929239.1"
                     /translation="MPAEGLLTMRSYGGTDIGRRRAINEDAFLCDDELGLWVVADGMG
                     GHAAGEVASQESIDTIFGMVRRGKLSLKLDGEPTEEKARAACRVLEGAVQAATYLVFA
                     MAELDRGKAGMGTTISAMMRFGDSLVLGQVGDSRIYRVRQGRAQQLTEDHTLIAWQLK
                     QGLITEDEARVSKQRNVITRAVGSRDYVQVDTSVWVTNPGDSYLLCSDGLHGYLQPEE
                     VTHALHERGERAVRSLIERANLRGGKDNITAVIVEVTSPQAVTERPGPASS"
     gene            2149..3213
                     /locus_tag="VFQ35_01070"
     CDS             2149..3213
                     /locus_tag="VFQ35_01070"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9929240.1"
                     /translation="MKPRRIGVVMGGMSAEREISLSSGRAVAEALIEADYDVVPVLLG
                     DDIDPLEALREAQIEVAFLALHGRLGEDGCVQGMLEWLGIPYTGSSVLASALAMDKQK
                     SKELFRLHNVPTPPYYVLDRRSVADLEEVHGSFGFPVIVKPRREGSSLGVSIARNEAE
                     LARAAELALAYDEHALVERYIVGREVHVGILGDRVLGAIEVAPRGGFFDYAAKYTPGE
                     TEYFMPARLSGARYRGVLNLAERAAQALDTEGAVRVDLMVTEGQNEYVLEVNTLPGLT
                     PTSLLPKIANAAGFTFGDLCEAILDRARLHTASELRQVPASSPVLAVPEAELDADEES
                     EVAVVRRGGARSASARARAR"
     gene            complement(3262..>5307)
                     /locus_tag="VFQ35_01075"
     CDS             complement(3262..>5307)
                     /locus_tag="VFQ35_01075"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9929241.1"
                     /translation="PFLSRAIEVSESRSKDTRDELLDEEHRAERRAERRARRLTALYA
                     ANTFGGAGGALFAAYAIVPTLGVNGTLFASALASAVVGLTAAYRGRAFEVPELETRAK
                     VDGKEKALRGTVEVELRAPSSADRARLAALAFASGALVFAAEVVFTHLLALIIGNSAY
                     AFGLILAAFLSSLFVGATLAAPLERRLSSGALALGLVATTLALLGSLPVWDDLPRLFA
                     VSGKDVVTFEGREAVRGLTALAVLLIPTSLMGLTFPLLLLRVAKYSGMGGWVGRLTAV
                     NTLGAVVGALATGYVVLPWLGSQSSLAAIGLAFGVSALVTLSWTTPLVRTLTASGVAL
                     GLFAWIAAPRWDLANLTSGANVYFEGHKTTQELVSIREDVHGGVTTVALSDGVYTLYT
                     NGKFQGNTGWEMNAQRFFAHYPSIFVNAFDATLVIGLGTGTTLGTLATYPWKSIDVAE
                     ISPAIVEAARQYFRGPNRGALGDRRVNLTHADGRNFLLVSDRKYDLISMELSSIWFAG
                     ASSLYSDEFYRLVAAHMQPGAVFQQWVQLHHIYKKDFATIVHTLRKNFRHVALFYGGG
                     QGILVASDAPLRASRERLKELQQRPNLIEVLPFGRPLEGLVDDVLVMNEGIDRFLARA
                     AAERNESLADWVSTDENLYLEYATPRGNVLPWATREELVAELQSFRDEDQIDALLTP"
BASE COUNT          958 a         1855 c         1670 g          824 t
ORIGIN      
        1 cggcgctatc tcgaacgacg aatgcatgcc ggcgagcggc agatcaccga agcgcgctcg
       61 gatgacgcga gtgtcgacgt ccggcgaacc gtaaagggac gtgccacgcc cggcgcagct
      121 caaatagacg ccgaaacgcg ccgccgcgcc gccggtctcg cgggcgaggc gcgcggtcgc
      181 cagttcgaga tcgctgcgag cagcgaccgg atcgcgcact gcgaacgcca tgagcaggcc
      241 ttcacgcacc tcttcggaaa cgacgattcc ttgacgcacc ggatcgatgc cttgaatgcc
      301 gcgcagcagc atgggcggca catcgctctc gtcggtcggc ttttgagcca gcgcgacgaa
      361 gagcagcggc tggtccttca agttcgcggt ggcgagcgag agcgcctcga gcgccttttc
      421 gcctccgagc tccaagacca tcggcccgcg cgtcgccgtg atcgggatga gcggtgtcag
      481 caaccggcat gctggcgagg ggcgaaccga aggaggagtg ccgcccttca agacgagcgc
      541 gccgatgttt ccgacccgcg cttttccgct cgaaaccagg gcgacgccgc cgtcgccggt
      601 ggttccggcg ccgaccagcg cgccgaagcg gaggctatgc agcgggtcga gcatgtgtgg
      661 caaaacgccg cgcggcctca agaacaagaa cgctgtcgac gaggagccgg cgtgcgcttc
      721 gagcgcttcc gccgtcgaca gcccaagatc gggtccgccc gtgtcatcga ttgagatagg
      781 ggtcgcagtg ccaccttccc acaccaccgc tgcaagcgcg ctttcgccct cgatttcgcc
      841 ccgttcggtc agcacgccgt gtccggcggc gacgagcgct ggcacccgac cgagcgcttc
      901 tgcgacgtga ctcgacactt cctcgaatga cgcaacgagc tcaccgctca cgaacacgag
      961 cgcgccgctc gctttgggca atcggcggat cgcttcaccc aagccccgcg caacacgggc
     1021 aggactctcg cctcgagcga ggaagctgag tgctctcgcg ctcatctgcc ggcacgataa
     1081 gcccgaacgc cgccctcgtg gaggggaaag cggtgcgagt ggcgaagcgc gtcgggtctt
     1141 gtaggacgga tctcacggca gacgcgctcg gacccttccc cgttcgcgcg aagtccccta
     1201 tcctctggga ataaatgccg gccgaaggtt tgctgacgat gcgttcctat ggcggcacgg
     1261 atatcggccg ccgccgggcc atcaatgagg acgccttcct ctgtgacgac gagctcgggc
     1321 tgtgggtcgt cgcggacggg atgggcggac acgccgcggg cgaagtcgcg agccaagagt
     1381 ccatcgacac catcttcggc atggttcgcc gtggcaaatt gtcgctcaag ctggacggcg
     1441 agcctacgga ggaaaaggct cgcgccgcct gtcgcgtgct cgaaggggca gttcaagccg
     1501 ccacctacct ggtctttgcc atggcggagc tcgaccgggg caaagccggg atgggcacga
     1561 ccatcagcgc catgatgcgc ttcggggatt cgctcgtgct tggccaggtg ggtgacagcc
     1621 gcatctaccg cgtgcgccaa gggcgagccc aacagctgac cgaggatcac acgttgatcg
     1681 cctggcagct caagcaaggt ctcatcaccg aggacgaggc gcgcgtcagc aagcagcgga
     1741 acgtgatcac cagggccgtg ggtagtcgcg actacgtcca ggtggacacc agtgtttggg
     1801 tcaccaatcc cggcgacagc tacctgcttt gcagcgacgg cctgcacggt tacctccaac
     1861 ccgaggaggt gactcacgcg ctgcacgagc ggggagaacg cgccgtgcgg tcgctcatcg
     1921 agcgcgcgaa cctgcgcggc ggcaaagaca acatcaccgc cgtcatcgtc gaggttacct
     1981 cgccccaggc cgtaacggaa agaccaggcc cggccagtag ttagcgctca cgttgcaggt
     2041 aatgccaaag ctctcgggag tgcccctccg ctcgctttgc tcggttcaga ttcttgcccc
     2101 aatggcggcg actccggtac cgctcacagg gtcagcgagg tatcccgaat gaaaccgaga
     2161 cgtattggtg tcgtgatggg cggcatgtcc gcggagcgcg aaatctccct gagctccggc
     2221 cgggccgttg cagaggccct gatcgaggcc gactacgacg tcgtgcccgt attgctcggg
     2281 gacgacatcg accccctcga agctctgcgc gaggcgcaga tcgaggtcgc attcctcgcg
     2341 ttgcacggcc ggcttggaga agacggctgc gttcagggaa tgctcgagtg gctcggtatt
     2401 ccgtacacgg gctcgtcggt cctggcgagc gcgctcgcga tggacaagca aaagtccaag
     2461 gagctcttcc gtctgcacaa cgtgccgacg ccgccctact acgtgctcga tcgtcgcagc
     2521 gtggccgatc tggaagaggt gcacggctcg ttcggcttcc ccgtcatcgt gaagccgcgc
     2581 cgcgaaggct cgagcctcgg cgtctcgatc gcccgcaacg aagcagagct cgctcgggcc
     2641 gcggagctcg ctctcgcgta cgacgagcac gccctggtcg agcgctacat cgtcgggcgt
     2701 gaggtccacg tcggcatcct gggcgatcgc gtgctcggtg ccatcgaggt cgcgccgcgc
     2761 ggcgggttct tcgattacgc cgcgaagtac acgccgggcg aaaccgagta cttcatgccg
     2821 gcgcgtctca gcggcgctcg ctatcgcggt gtcttgaacc tcgccgaacg cgccgcccaa
     2881 gccctcgaca ccgaaggcgc cgtgcgcgtc gatctcatgg tcaccgaggg ccagaacgaa
     2941 tacgtgctcg aggtgaacac gctgcccggc ctcacgccga cgagcctctt gccgaagatc
     3001 gcgaatgcgg ccggcttcac cttcggcgat ctctgcgaag ccattttgga tcgcgcgcgc
     3061 ctgcacaccg cgtccgagct gcgccaggtt ccggcgagca gccccgtcct cgcggtaccc
     3121 gaggctgaac tcgacgccga cgaagagtcc gaagtcgccg tagttcggcg cggcggtgct
     3181 cgtagcgcat cggctcgcgc ccgcgcccgc tgagcgtcgc acccgcgccg agcgtcgcac
     3241 ccgcgcgcaa tgagcgtcgc gtcagggcgt gaggagcgcg tcgatctggt cttcgtcgcg
     3301 gaagctctgg agctccgcga cgagctcctc gcgcgtcgcc cacggcagca cgttgccgcg
     3361 cggcgtcgcg tactcgagat agaggttctc gtcggtggac acccaatcgg cgagcgactc
     3421 gtttcgctcc gccgccgccc gagcgagaaa acggtcgatc ccttcgttca tcacgagcac
     3481 gtcgtcgacc agcccctcga gcgggcgtcc gaacggcaac acctcgatga ggttcggacg
     3541 ctgctgcagc tccttgagcc gctcccgcga agcgcgcagc ggggcatccg acgcgaccag
     3601 gatgccctga cctccgccgt aaaacagcgc cacgtggcgg aagttctttc gcagcgtgtg
     3661 aacgatggtc gcgaagtctt tcttgtagat gtgatgcagc tgcacccact gctggaacac
     3721 cgcccccggc tgcatgtgcg cggcgacgag ccgataaaac tcgtcgctgt acaggctcga
     3781 agcacccgcg aaccagatgc tcgacagctc catgctgatg agatcgtact tgcgatcgct
     3841 caccaacaag aagttgcgac cgtcggcgtg ggtcaggttc acgcgtcggt ctccgagcgc
     3901 gccgcgattg ggtccgcgaa aatactgccg cgcggcctcg acgatggcgg gcgagatctc
     3961 ggccacgtcg atcgacttcc acggatacgt cgccaacgtg ccgagcgtcg tgccggtgcc
     4021 caacccaatc accagcgtcg cgtcgaacgc attcacgaag atcgaggggt agtgggcgaa
     4081 gaagcgctgt gcgttcatct cccagcccgt attcccctgg aacttcccgt tggtgtagag
     4141 cgtgtaaact ccgtccgaca gcgcgacggt cgtgactccg ccgtgcacgt cttctcggat
     4201 gctcacgagc tcttgtgtcg tcttgtgccc ttcgaagtag acgttcgcgc cgctcgtcag
     4261 gttcgccaaa tcccaacgtg gcgctgcgat ccaagcgaat agccccaaag ccactccgct
     4321 cgccgtcagc gtccgcacga gcggcgtagt ccagctcagc gtcacgagcg ccgacacacc
     4381 gaatgcgagc ccgatcgcgg cgagcgacga ctgtgagccg agccacggca gcaccacgta
     4441 tccggtagcg agcgccccga cgactgcgcc caacgtattc acggccgtga gccgtcccac
     4501 ccagccgccc atgcccgaat acttcgccac gcggagcaaa aggagaggga aggtgagccc
     4561 catcagactc gtcggaatca ggagcaccgc gagcgcagtc agcccgcgca ccgcttcgcg
     4621 cccttcgaac gtcaccacgt ctttgccgct caccgcaaac agccggggca gatcatccca
     4681 gaccggcaac gaccccagca gcgccagcgt ggttgccacc agcccgagcg ccaaagcgcc
     4741 cgacgacagc ctgcgctcga gcggcgccgc gagcgtcgct cccacgaaca acgaactcaa
     4801 aaacgccgcc aggatcagcc cgaaagcgta cgcgctgttc ccgatgatca gcgccaacaa
     4861 gtgcgtgaac accacctctg ccgcaaagac caacgcgccc gaagcgaaag ccaaggccgc
     4921 gagccgcgcc cggtccgcag acgaaggcgc acggagctcc acttccaccg tgccccgcag
     4981 tgccttctcc ttcccgtcca ccttcgcccg cgtctcgagc tccggcacct cgaacgcgcg
     5041 gccccgataa gccgcggtca gccccacgac tgcactcgcg agcgccgacg cgaacaacgt
     5101 cccgttcacg cccagcgtcg gcacgatcgc gtacgccgcg aacagcgctc caccagcccc
     5161 cccaaacgta ttcgccgcgt acaacgccgt cagtcgccgc gcgcgccgct ccgcacgccg
     5221 ctccgcgcgg tgctcctcat ccagcagctc gtcccgtgtg tctttggagc gcgactcgct
     5281 cacctcgatc gcgcgcgaca aaaacgg
//
LOCUS       DASTKS010000049         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1790214, whole genome shotgun sequence.
ACCESSION   DASTKS010000049 DASTKS010000000
VERSION     DASTKS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790214"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VFQ35_01080"
     CDS             <1..1445
                     /locus_tag="VFQ35_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9929242.1"
                     /translation="PSTEVNPALAARLGRPRTVLDWGCGAGLNGMLARSVGAKVVGVE
                     ANPQLAARARRVLEEVVELEPGDFEGLRGALGERRFDLVFLPTALERVAEPGRLLREA
                     ASYLEPEGRILCALRNPEAWTRRLGLSDEGFGFTRAGGGEARLHDVEAARGWVAAAGL
                     ELLEVALTPLVVRALRPLLPEELYTSLAKDEERASGFRDWSVYEAYRHSLLPLETKAA
                     ALAPGLLAYQYVLVLRKPVEKRPLSLTVGMLTMDEEPSVANMIQEIRRYAPDARILCV
                     DSSVKDQTPVIAERLGARVLRQLPPRGHGPAMELLMYEAARESDALIYLDCDFTYPAE
                     NIVKIREILQGGADVVNAARTRTRPQAMPLANYLANKTFVWCARALDGVPISDLHSGM
                     RGYRCSVIRSFAFDGSGDALPIDTLLWPARSGYRVVEIPIRYQERIGASKLRKLAGTA
                     WTFVRLARTLSVGQRSSGSYERWGSDEAEP"
     gene            1442..2038
                     /locus_tag="VFQ35_01085"
     CDS             1442..2038
                     /locus_tag="VFQ35_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HET9929243.1"
                     /translation="MNPCVILDRDGTLIDVVRDEETGTVNVAFHPSQLVLLPGVVSGL
                     QSLVAHGFKLGIATNQPGPAKGQFSRHAVQRTNDALVAMLRAEGIPIAAVAACLHHPT
                     GAPDGDRELITDCNCRKPKPGLLHALDESMHILRAESWMIGDSRADLEAAHAFGVRSA
                     LVFSTQRCELCPLRAGPSGAPDVVAPRFDDVVARLLSG"
     gene            complement(2062..3027)
                     /locus_tag="VFQ35_01090"
                     /pseudo
     CDS             complement(2062..3027)
                     /locus_tag="VFQ35_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
     gene            3115..4134
                     /locus_tag="VFQ35_01095"
     CDS             3115..4134
                     /locus_tag="VFQ35_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9929244.1"
                     /translation="MTTMLRMLRLFIVTWFETSFARLRGGRLLPSWNFQFEWVVRFLR
                     RDFIETSLWPYGLLRRELDGRRYPSRALPKVAAQDSALGGVPVVIFTPKHAEPARTLL
                     YFHGGSYIFGSARTTHADLLARIALGANLRVVAPNYRLAPEHPYPAALEDALAVFAEL
                     AQRTAPSEIVLMGDSAGGNLALVLQIALRDRARARGDAKAGADDHRQAQAKASILISP
                     WLDLSASLPSCRKNDRYDYGQTEFLLRHARDFAGQVALDDARVSPLYADLGGLAPVVV
                     LVGGAERLHDEAIAFVDRARSAGVRVALDVAPEMPHLAPALADFHPAAKRALETLIAH
                     ARDSS"
     gene            complement(4199..5005)
                     /locus_tag="VFQ35_01100"
     CDS             complement(4199..5005)
                     /locus_tag="VFQ35_01100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9929245.1"
                     /translation="MNAPSSLPGPGSAATFYDRVWTDWGHLDERSPAALHRRRALLRL
                     VREHCRSSRRMLEVGCGQGALLRDLAREFPASHVHGADVSAESLARARERCPQSTLFE
                     LDLDSPYFDEQASSRSGQFDLVVCSEVLEHLRNDRLALERLGTLLAPGGRLILSVPAG
                     ERTRFDRAIGHLRHYTEGQMAERLQDAGFEAERVFSWGFPFHSFYRWVVKWAARATLQ
                     ASGAPRNSNRGGPNALLSAAYSAFALGLRPLYLLNSARCGPQLFAVARKP"
     gene            5078..>5855
                     /locus_tag="VFQ35_01105"
     CDS             5078..>5855
                     /locus_tag="VFQ35_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929246.1"
                     /translation="MTRVTALLDALLSALEVRVADPDARIRMSAALLAWVILLSGFVA
                     LLWPMLRDFSTYGFHDWDAHATYRYITALSVRRYHEGPWWHPWLCGGVPAWGYVEGAP
                     NLISPYLPVYLFADLRTALRVEVVGNGLIGLAGAYVLARRSCESRAFAAFFAALFVLN
                     GRWALQAAVGHTWHLQYAVTPWVFYAFERALAAPWRAARFALLAGALLAYVCYAGGIY
                     PLPHTALFLCAYAFMRALFERTFKPIAVLALAGVSALGLAA"
BASE COUNT          886 a         1956 c         1988 g         1025 t
ORIGIN      
        1 acccgagcac ggaagtgaat ccagcgctcg cggcgcgctt gggtcggccg cgcacggtcc
       61 tcgactgggg ctgcggtgca gggctgaacg gcatgctggc gcgcagcgtc ggcgccaagg
      121 tggtgggggt cgaggcgaac ccgcagcttg ccgctcgcgc gcgccgcgtg ctcgaagaag
      181 tcgtcgagct cgagcccgga gatttcgaag ggcttcgcgg cgcgctcggg gagcggcgct
      241 tcgatctcgt atttttgccg actgcgctcg agcgtgtcgc ggagcccggt cggttgctgc
      301 gcgaagccgc gagctacctc gaaccggagg gtagaatcct gtgcgcgctg cgcaaccccg
      361 aggcgtggac gcgccggctg gggctctcgg acgagggttt cggcttcact cgcgccgggg
      421 gcggcgaagc ccggcttcac gacgtcgaag ccgcgcgtgg ctgggtcgca gcggcggggc
      481 tcgagctact cgaagtggcg ttgacgccgc tcgtcgtccg cgcgttgcgc cccctgctcc
      541 ccgaggagct ctacacgagc cttgccaagg acgaagagcg agcgagcggc tttcgcgatt
      601 ggagtgtcta cgaggcctat cgacatagct tgctgccgct cgaaaccaag gcggccgcgt
      661 tggctccggg gctgctggct taccagtacg tgctcgtgct gcgaaagccg gtagaaaagc
      721 gtccgctgtc gctcacggtg gggatgttga cgatggacga ggagccgagc gtcgcgaaca
      781 tgatccaaga gatccgtcgc tatgcaccag atgctcgcat cttgtgcgtc gacagctcgg
      841 tcaaagacca aactccggtc atcgccgaac gactcggggc gcgggtgctt cgtcaattgc
      901 cgccgcgcgg ccacggcccc gcgatggagc tcttgatgta cgaggcggcg cgcgagagcg
      961 acgcgctcat ctacctcgat tgcgacttca cttatcctgc cgagaacatc gtcaagatcc
     1021 gggagatttt gcagggcggc gccgacgtcg tgaacgcggc tcgtacgcgc acgcggccgc
     1081 aagcgatgcc gctcgccaac tacctcgcca acaagacgtt cgtttggtgc gcgcgagccc
     1141 tcgacggagt gcccatctcg gatctgcaca gcggcatgcg cggctatcgc tgcagcgtga
     1201 ttcggagctt tgctttcgac gggagtggtg acgcgctccc gatcgatacg ctgctctggc
     1261 cggctcgctc cggctaccgc gtcgtcgaga tcccgatccg ttatcaggag cgcatcggcg
     1321 cctccaagct ccgcaagctc gcgggaacgg cgtggacctt cgtacgtctc gcccgcacgc
     1381 tgagcgtcgg ccagcgctcg agcggcagtt acgagcgctg gggtagtgac gaggcggaac
     1441 cgtgaatccg tgtgtgatcc tggaccgaga cggtacgctg atcgacgtcg ttcgcgacga
     1501 ggagaccggc accgtcaacg tcgcatttca cccgtcgcag ttggtgcttt tgcccggtgt
     1561 cgtcagcggg ctccagagct tggtcgcgca cggcttcaag ctcggcatcg cgacgaacca
     1621 gccgggcccg gcgaagggcc agttttctcg ccatgccgtg cagcgcacca acgacgcgct
     1681 cgttgcgatg ttgcgcgccg aaggcatccc catcgcggcg gtcgcggcgt gccttcatca
     1741 cccgacgggc gctccggacg gcgaccgcga gctcatcacc gactgcaatt gccgaaagcc
     1801 gaagccgggg ctcttgcacg cgctcgatga gagcatgcac atcttgcgcg ccgagagctg
     1861 gatgatcggc gactcacgcg ccgatctcga ggctgctcat gccttcgggg tgcggtccgc
     1921 gttggtgttc tcgacgcaac gctgcgagct ctgtccattg cgagccggtc cttcgggagc
     1981 accggacgtc gtcgcgccgc gcttcgacga cgtcgtcgcg cgcttgctct ctggttgagc
     2041 tgtcggagca gcccagcgac gtcgcgcgcg agcgagcgcg ctcgggtcgg catagccgac
     2101 gcgcagggcg gcctcttttg cggccgcacc ttgctgcatg agttcctcgg ctcgggcgcg
     2161 gcgttcctcg tcgatgagct gttgaaagct ggtgccttcg cggcgaagga gccgctgcag
     2221 gctgcgcgag ctcacccgaa gccgctccga gagctccgcg agcgaggtgc gcaccgggtt
     2281 ctcggctagg ctttcgcgca gcgccgaacg aactcggccg acgaagtcga gccgctctgg
     2341 caaggcgctg agcgctcgct cgcactgcgt ttggatcgcc gaactcagca aatcgcgaag
     2401 cacgtggatg gggcggtcta ccacgtctgc ggagagcacg atgcggctct gagttcgccc
     2461 gaagagtgga gcgatgccga aatggcgagc gagtcgagag gtctcgccga gcggggcgtg
     2521 agcaaactcg aggctcaccg gcgtgaccgc ctcgtcgacc aggtagcgag cgccggcaag
     2581 cacctcggcg agggcgcact cggcgaagac cgcgcgagcg agcgcgctgg tccccgggtg
     2641 tttgaacgcg acgctgagcg gccctttgcc gggcgaggcg gtgaagcgct ctccgtcgcc
     2701 ccaaattcgc tggaattcga tcgagcgggt gagcccttgg cggtacgtgc cgctcaccag
     2761 cagcagtagc cctgcggcgc cgtaagcttg gtcgtcgcgt acggccgcaa gctcgagccc
     2821 tagctcgtgc gttgggaccg actcggcaag cgcctcgagc aggcgctcta cttcggagta
     2881 ggggatgcgc gcgttcggcg acttcaaggg cgcggacgga atgttcgcgc gccgcagcag
     2941 actctcggag tcgagcccac ggcgctcacc caggccgagc agccgcgcaa cgagccgcgc
     3001 ggacatggcg tcgcccgaag cgctcattcg gtggcctccc tcgttggcgg tcgcggcctc
     3061 gcgaagtgac gcgaaaggcc cgcttcggct tcggagccgc gctgtaggtt ctgaatgacg
     3121 accatgctgc ggatgctcag gctcttcatc gtcacgtggt tcgagaccag ttttgcacga
     3181 ctgcgagggg ggcgtttgct tccttcgtgg aacttccaat tcgagtgggt cgtgcgcttc
     3241 ctgcggcgcg acttcatcga aaccagtctt tggccatacg gactcttacg gcgcgagcta
     3301 gacggccggc gctacccgag ccgcgccctc ccgaaggttg cggcgcaaga ctctgcgctc
     3361 ggcggcgtgc cagtcgtgat cttcactccg aagcacgccg agcccgcgcg cacgctgctc
     3421 tacttccacg gcggctccta tatcttcggc tcggcccgca ccactcacgc cgacctgctg
     3481 gcgagaatcg ccttgggcgc gaacctgaga gtcgtcgcgc cgaactaccg gttggctccc
     3541 gagcacccct atccggcggc actggaggac gcgctagccg tcttcgcgga gctcgcccag
     3601 cgcaccgcgc ccagcgagat cgtgctgatg ggcgactctg ctggcgggaa cctcgcgctc
     3661 gtgttgcaaa tcgcactgcg cgatcgcgct cgcgcccgcg gcgacgcaaa ggctggcgcc
     3721 gacgaccacc gacaggctca ggcgaaagca tccatactca tctcgccgtg gctcgatctt
     3781 tcggcgagcc taccgtcgtg ccgaaagaac gatcgctacg actacgggca aaccgagttc
     3841 ctgctgcgtc acgcgcgtga ctttgccggc caggtggcgc tcgacgatgc gcgcgtctcg
     3901 ccgctctacg cagatctcgg gggccttgcg cccgtcgtag tgctggttgg cggcgccgaa
     3961 cgcttgcacg acgaggcgat cgcattcgtg gatcgcgccc gcagcgccgg ggttcgcgtc
     4021 gcgctcgacg tcgcgcccga gatgccgcac ttggctcccg cgctcgcgga ttttcaccct
     4081 gcggcgaagc gagcgctcga gacactcatc gctcacgcgc gagactcttc ctagttgcgg
     4141 ccgaacgtag cggcatcggt cgacctgcgc gcggacccgt gcgccggtgc tcgttcgttc
     4201 acggtttccg cgcgaccgcg aagagctgtg ggccgcagcg cgcgctgttc agcaagtaga
     4261 gtggtcgcag acccagcgcg aacgccgaat aagcggcgct caagagcgcg ttgggccccc
     4321 cgcggttcga gttacgcgga gcgccgctcg cttgcagcgt ggcgcgtgcc gcccatttga
     4381 cgacccagcg gtagaagctg tgaaatggaa agccccacga gaacacgcgc tcagcctcga
     4441 agccggcgtc ttgaaggcgc tccgccattt gcccttcggt gtagtgacgc aaatgaccaa
     4501 tggcgcgatc gaagcgcgtg cgctctcccg ccgggacgct caggatgagc ctaccgccgg
     4561 gcgcgagcag tgtgcccaac cgctcgaggg cgagacggtc gttgcgaaga tgctcgagca
     4621 cctcgctgca gacgacgaga tcgaactgcc cggaccgact cgacgcttgc tcgtcgaagt
     4681 aaggcgaatc gagatcgagc tcgaacagcg ttgactgcgg acagcgctcc cgagcccggg
     4741 cgagcgactc cgcgctgacg tcggcgccgt gcacgtgcga ggcgggaaac tctcgggcca
     4801 aatcgcgcaa cagcgcgcct tggccgcagc cgacctcgag catccgtcgg ctgctacgac
     4861 agtgctcccg aacgagtcgc agcagcgcgc gcctgcgatg cagcgccgcg gggctccgct
     4921 cgtcgaggtg tccccagtcg gtccacacgc ggtcgtagaa ggtcgcagcg gaacccgggc
     4981 cgggcagcga agagggcgcg ttcacgttcg aaatgcgtag ctcgcggccg ttgcgccgca
     5041 agtcgcctca cagcagattc ctttgctaca agggggcatg actcgggtca cggcgctgct
     5101 cgacgccctc ctttcggcgc tcgaagtgcg cgtcgcggac cccgacgcac ggatccggat
     5161 gagcgccgcg ctgctcgcct gggtgatttt gctcagtgga ttcgtcgcgc tgctctggcc
     5221 aatgctgcgc gatttttcga cttacggttt ccacgactgg gatgctcacg ccacttaccg
     5281 ctacatcacg gcgctcagcg tgcggcgcta tcacgaaggc ccgtggtggc acccgtggct
     5341 ctgcggaggc gtgccggcct ggggttatgt cgaaggcgcg cccaacctga tttcgccgta
     5401 cctgccggtg tatctattcg cggatcttcg tactgcactg cgcgtggagg tggtcggcaa
     5461 tgggctgatc gggctcgcgg gtgcgtacgt tctggcccgg cgctcgtgcg agagccgcgc
     5521 gttcgccgct ttcttcgctg cgctcttcgt cttgaacggt cgctgggcgt tgcaggctgc
     5581 cgtcgggcac acgtggcacc tgcaatacgc cgtcacgcct tgggtcttct acgccttcga
     5641 gcgcgcgctc gcggctccct ggcgcgctgc tcgcttcgcg ctgctcgccg gcgcgctcct
     5701 cgcctatgtc tgctatgcgg gcggtatcta tccgctcccg cacacggcgc tgttcctgtg
     5761 cgcctacgcc ttcatgcgtg cgctcttcga acgcaccttc aagcccatcg ccgtgctcgc
     5821 gttggcaggt gtctcggcgc tcgggctggc ggcgc
//
LOCUS       DASTKS010000050         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_229668, whole genome shotgun sequence.
ACCESSION   DASTKS010000050 DASTKS010000000
VERSION     DASTKS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229668"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VFQ35_01110"
     CDS             <1..1567
                     /locus_tag="VFQ35_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929247.1"
                     /translation="AARLASDRLLSAVESIHGMLALYDASDKLVLCNSACRELFARGI
                     DGPIVERTYAEIIDGSIAAGLFDLGTESPSDFRARCIGYHENPVGTLELKTTDGRNLR
                     VTDSRTLEGGIISTIWDVTDDVQYEADLKLARMQAEAASSAKSEFLASMSHELRTPLN
                     SILGFAQLLQRDKKTPLTERQGEKLDYVIKGGEHLLRLIDDVLDLSRIEAGHVAVSPE
                     PVAIAEVLAEVQTTLDPMAARAGISLSVEPLPTGPREVIADRTRFAQILINFGSNAVK
                     YGKQGGHATFSVTYNENYARVSLTDDGIGIPYEHQEKVFQPFHRAGQETGPIQGTGIG
                     LAITRRLAELMGSSVGFSSIPGQGSNFWIELPVHLAPSRRPAASNEQGLSTSALSGSE
                     GERYTVVYIEDNPSNIAFMQELIAELDRVTLISVPTAEVGIELVRARHPDVVIMDINL
                     PGMSGYEATRKLREWPETKEIPVIALTAAAMAGDRKRFADAGFYRYLTKPVRVPELLN
                     TLEELLSRPSAEL"
     gene            complement(1553..>2129)
                     /locus_tag="VFQ35_01115"
     CDS             complement(1553..>2129)
                     /locus_tag="VFQ35_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9929248.1"
                     /translation="RPPKATGQLMFNDGLGAICFPMPGQRFRYGVQLSEGLDAVPNLE
                     LLRSLLASRPPSKPDEVERVDWSTVIHFERRLARSFGRGRVWLAGDAAHVTSPFGGQS
                     MNVGLQEAHRLANRIADTLQGKNGHGGLEAYSAEFEREWHKLFGVNVRFDVAPNAPRW
                     LSTYARRIVPALPASGDDLEKMLAQLGLKLS"
BASE COUNT          395 a          714 c          691 g          329 t
ORIGIN      
        1 cgcggctcgc ctcgcgagcg accggttgct gagcgcggtc gagagcatcc acggaatgct
       61 cgcgctctac gacgcgagcg acaagctcgt gctgtgcaac agcgcgtgcc gagagctctt
      121 cgcgcgcggc atcgacggcc ccatcgtcga gcggacgtac gcggagatca tcgacggcag
      181 catcgccgcg ggcctgttcg atctgggcac cgagtcgccc tccgacttca gagcgcgctg
      241 catcggctat cacgagaacc cggttggcac cctcgagctc aagacgaccg acgggcgcaa
      301 cttgcgcgtc accgacagtc gaaccctgga gggcggcatc atcagcacga tttgggacgt
      361 caccgacgac gttcagtacg aagcggacct caagctcgcg cgcatgcagg ccgaggccgc
      421 gagcagcgcc aagagtgagt tcttggcttc gatgagccac gagctgcgca cgccgctgaa
      481 ctcgatcctc ggcttcgcgc agctccttca acgcgacaaa aagactccgc tcacggagcg
      541 gcaaggcgag aagctcgact acgtcatcaa gggcggtgaa cacctgctgc gcttgatcga
      601 cgacgtgctc gatctgtcgc gaatcgaggc gggacacgtc gcggtctcac cggagccagt
      661 cgcgatcgcc gaggtgctcg ccgaggtgca gacgacgctc gatccgatgg ccgcgcgcgc
      721 cgggatcagc ctttcggtcg agccgctgcc gacgggaccc cgtgaagtca tcgccgatcg
      781 cacgcgcttc gcgcagatcc tcatcaactt cggctcgaac gcggtgaagt acggaaagca
      841 gggaggccac gccacgttct ccgtgactta caacgagaac tacgcgcgcg tgagcttgac
      901 cgacgatggc atcggaattc cctacgagca ccaagagaag gtgtttcagc cgttccatcg
      961 cgcgggtcag gagacggggc cgatccaagg caccgggatc ggcctcgcca tcacgcgccg
     1021 cctcgcggag ctgatgggca gttccgtcgg cttcagcagc atcccaggac aaggctcgaa
     1081 cttctggatc gagctgccgg tccacctcgc gccgagccga cgaccggccg cgtcgaacga
     1141 gcaaggccta tcgacctccg cgctgagcgg gagcgaaggc gaacgctaca cggtcgtcta
     1201 catcgaggac aacccatcca acattgcgtt catgcaggag ctcatcgccg agctcgatcg
     1261 cgtgaccctg atcagcgtgc cgacggcaga ggtgggcatc gagctcgttc gagcgcgcca
     1321 ccccgacgtc gtgatcatgg acatcaacct gccgggtatg agcggctacg aggcgacgcg
     1381 aaagctccgt gagtggcccg agaccaagga aattccggtc atcgcgctca cggcagcggc
     1441 catggctggc gaccgcaagc gcttcgccga tgcagggttc taccgctact tgaccaagcc
     1501 ggtgcgggtc cccgagctcc tcaacacgct cgaggagctg ctatcgcgcc cgtcagctga
     1561 gctttaggcc gagctgcgcc aacatcttct cgaggtcgtc tccggacgcg ggaagcgcgg
     1621 ggacgatgcg acgcgcgtac gtcgacagcc agcgcggcgc gttgggcgcg acgtcgaagc
     1681 ggacgttcac cccgaagagc ttgtgccact cgcgctcgaa ctcggccgag tacgcctcga
     1741 gtccgccgtg gccgttcttg ccctgcagcg tatcggcgat gcggttcgcg aggcggtgag
     1801 cctcttgcag gccaacgttc atactttgtc cgccgaacgg gctcgtcacg tgcgccgcgt
     1861 cgccggcgag ccagactctt ccgcgaccga agctccgcgc gaggcggcgc tcgaagtgaa
     1921 tcacggtgct ccaatcgacg cgctcgacct cgtcgggctt cgacggtggc cgactggcga
     1981 ggagcgagcg aaggagctcc aggttgggca ccgcgtcgag cccctccgaa agctgcacgc
     2041 catagcgaaa cctttgcccc ggcatcggga agcaaatcgc gccgaggcca tcgttgaaca
     2101 tgagctgccc ggtcgccttc ggcgggcgg
//
LOCUS       DASTKS010000051         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1122081, whole genome shotgun sequence.
ACCESSION   DASTKS010000051 DASTKS010000000
VERSION     DASTKS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122081"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ35_01120"
     CDS             complement(<1..258)
                     /locus_tag="VFQ35_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929249.1"
                     /translation="MRLVGALGLALGLFVRSASAEPIVVDRAIARFWAPDTGGVRSPH
                     FIYERVLSFEARLEALSDSIQDTGGEPYRDRHLTAALERHVA"
     gene            complement(255..1058)
                     /locus_tag="VFQ35_01125"
     CDS             complement(255..1058)
                     /locus_tag="VFQ35_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HET9929250.1"
                     /translation="MSDPDKQSSEAGNAPVRDVPVEWEALEDAFENNAPEVHSYLHLV
                     TGEVLRVVDGIADPEMHTRIASDATYMRVEPVSSREQYRWMERFIPMVEEPALRDQLT
                     AAIDGKGAFRRFKDVLMNFSAERERWFTFRSERLRVFMEAWLATHGLKAVPRVNWTPE
                     PSRPREPMPISQEQLGARRSRSAEALRKQLRELADTLGARDLEKVTAFAEFVKARRAA
                     RVFPHRHEGGAGVVGEGEGEGEHDHGDSDDAGSDDEPSPSSRPRAAASK"
     gene            complement(1069..2628)
                     /gene="glpK"
                     /locus_tag="VFQ35_01130"
     CDS             complement(1069..2628)
                     /gene="glpK"
                     /locus_tag="VFQ35_01130"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET9929251.1"
                     /translation="MKGRSGAKSVRRGFLVLSSAVSRYLLAIDQGTTGSTALVMGTDG
                     VTLAHANFEFSQHFPQPSWVEHEPEQIWESVTRAVSEALRRAQIRASDIAAIGITNQR
                     ETTLLWDRESDRPLHRALVWQDRRTAPLCDALRARGHEARVRATTGLVLDPYFSGTKI
                     NWLLEHVPGARESAERGDALFGTVDSYLVWRLAGGARRSAPHVTDVTNASRTLLMNLA
                     TLAWDEAMCEMLSVPRAMLPRIVPSAAVVATTRDVPGLPDGIPIAGMAGDQHAALFGQ
                     ACFGLGEAKCTYGTGAFLLANTGARPVMSRFGLLTTLAWQIGNEVSYALEGSSFVAGA
                     AVQWLRDGLGIIESAPAVEALAATVESSDGVVFVPALAGLGAPYWDAAARGLITGITR
                     GTTRAHLARATLEAIAFQVDDLLRALAEDLGHPVAVLRVDGGAAANDLLMQLQADLAG
                     VSVERPNELESTARGAAMLAGVGAGIFANGRDAAKMVKIDRHFEVRMEASERQRRLVA
                     WQDAVRRARST"
     gene            2988..>3583
                     /locus_tag="VFQ35_01135"
     CDS             2988..>3583
                     /locus_tag="VFQ35_01135"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9929252.1"
                     /translation="MARILLSTPDGQQAVELRDQNSLGRHPSNSIQLLDKIVSKEHCR
                     IERRANGYVLRDLGSLNGTYVNGERVNGEQDLRHGDDIALGSTRGRFDDGTGRPVAPP
                     APYGSPIASPGAPPAWAPPPSVGTPQSLNVAQPVPSHVQAPPSLGYAAPYSQGPTAQF
                     AQTPAAPHQPGGPLGTVAMPQVGGPGQTRVGVDLNDRSR"
BASE COUNT          605 a         1279 c         1119 g          580 t
ORIGIN      
        1 ggccacgtgc cgctcgagcg ccgcggtcag gtgccgatca cggtacggct ccccccccgt
       61 atcttggatt gagtccgaga gggcttcgag gcgcgcctcg aacgagagca ctcgctcgta
      121 aatgaaatgg gggctgcgca cgccgcccgt atccggcgcc caaaagcggg cgatggcgcg
      181 atcgaccacg attggctcgg cgctcgcgct ccgcacgaac aacccgagcg cgagaccgag
      241 agcgccgact agcctcattt cgatgcggcg gcgcgtggac gcgacgacgg ggacggttcg
      301 tcgtcactgc cggcatcgtc cgaatcgcca tggtcgtgct cgccctcgcc ctcgccttcg
      361 ccgacgacgc ccgctccacc ttcgtgacga tgcggaaaaa cgcgcgccgc gcggcgagct
      421 ttgacgaact cggcgaaggc agtgaccttc tccaagtcgc gcgcgcccaa cgtgtcggcg
      481 agctcgcgca gctgctttct gagcgcttcg gcgctgcggc tacgccgggc cccgagctgc
      541 tcttgcgaaa tcggcatcgg ctcgcgtggg cgcgaaggct ccggcgtcca gttcacgcgc
      601 ggaacggcct tcaacccgtg cgtcgcgagc cacgcttcca tgaaaacgcg caggcgctcg
      661 ctgcgaaaag taaaccagcg ctctcgctcc gcgctgaagt tcatcagcac gtccttgaaa
      721 cgccggaacg cccccttgcc gtcgatggct gccgtgagct gatcgcgcaa agcgggctcc
      781 tcgaccatcg ggatgaagcg ctccatccag cgatactgct cgcgagagct cacgggctcg
      841 acccgcatgt acgtcgcgtc cgaagcgatg cgcgtatgca tttccggatc cgcgattccg
      901 tcgaccacgc gcagcacctc tccggtcacg aggtgcaagt agctgtgcac ttccggcgcg
      961 ttgttctcga aggcatcttc gagcgcttcc cactcgacgg gaacgtcgcg cacgggcgcg
     1021 ttccctgcct ccgagctctg cttgtccgga tccgacatgt tttctcctct acgtgctgcg
     1081 cgcccgacgc accgcgtcct gccaggctac caaacgcctc tgtcgctcgc tggcttccat
     1141 tctgacttcg aaatggcggt cgatcttgac cattttcgca gcatctcgtc cgttcgcgaa
     1201 aatacccgcg ccgacgcctg cgagcatggc cgctccccgc gccgtcgact ccaattcatt
     1261 cggccgctcg accgataccc ctgcgaggtc ggcctgcagc tgcatgagca ggtcgtttgc
     1321 ggccgctcct ccgtccacgc gcaggactgc caccggatgg cccaaatctt cggccaaggc
     1381 gcgaagcaga tcgtcgactt gaaacgcgat cgcctcgagc gtggcccggg cgagatgggc
     1441 gcgcgtcgtg ccgcgcgtga tgccggtaat gagaccacgc gccgcggcat cccagtatgg
     1501 cgcgccgaga cccgccagag cgggaacgaa cacgactccg tcggacgact ccacggtcgc
     1561 ggcgagcgcc tcgactgcgg gagcgctctc gatgatgccg aggccgtccc gtaaccactg
     1621 cactgccgcc cccgccacga acgagctccc ctcgagcgcg taactgactt cgtttccgat
     1681 ttgccaagcc aacgtcgtga ggagaccaaa acgactcatc accgggcgag cccccgtgtt
     1741 ggcgagcaaa aaagcaccag tgccgtaggt gcacttcgcc tcacccaagc caaaacacgc
     1801 ctgtccgaac agcgcggcgt gttggtctcc cgccatcccg gcgatcggaa taccgtcggg
     1861 caggcccggc acgtcgcgcg tcgtcgcaac cacggccgcg ctcggaacga tgcgaggcaa
     1921 catcgcgcgc ggcacggaga gcatctcgca catcgcttcg tcccaggcga gcgttgcgag
     1981 attcatcagc aacgtgcggg aagcgttcgt cacgtcggta acgtgcggcg ccgagcgacg
     2041 agcgccgccc gcgagccgcc acaccagata cgagtcgacc gtgccgaaca acgcgtctcc
     2101 gcgctcggcg ctctcgcgcg cgcccggcac gtgctcgagc agccaattta tcttggtgcc
     2161 agagaaatac ggatcgagga cgagaccggt cgtcgcacga acacgcgctt cgtgcccgcg
     2221 agcccgcaac gcatcgcaga gcggggccgt gcggcgatct tgccaaacga gcgcgcggtg
     2281 taggggtcgg tcgctctcgc gatcccaaag gagcgtcgtc tcgcgttgat tggtgatccc
     2341 gatcgccgcg atgtcgctcg ccctgatttg cgcgcgacgg agcgcttcgc tcacggctcg
     2401 cgtaacgctc tcccagatct gctccggctc gtgctcgacc cacgatggct gcggaaagtg
     2461 ctgtgagaat tcgaaattgg cgtgcgccag cgtgacgccg tcagtgccca tgacgagcgc
     2521 cgtcgagccc gtcgtgccct gatcgattgc gaggaggtag cgactcaccg cggacgacaa
     2581 taccagaaag ccgcgacgca ccgacttcgc acctgagcga cccttcattt cgttgcgtag
     2641 atgcgctccg aacccgcgcc gccgtttcac gccgaggcgt gggcgaggct ggaggatccc
     2701 ctcgatcgtc gagagagaga gtcccgatac ttggttcgtg tggcggtcgg ggcccggccc
     2761 gaattacacc gcttttgcgt tgtcgcggag aacgagggga tacatcgcac accctggagg
     2821 ccaatgtgtg agtgcccttt tttggccctg ggcacgccgc gcggcgcacc gagcgaccgg
     2881 cccacacgcc agacgagacc gctcgatctc cgcaagaatg tgcgagctcc gctcgcgggc
     2941 gatacccttg ctgacgcctg cgtcgaacgg tacgctcgtc ctccggtatg gcgcgcattc
     3001 tgctttcgac cccggacggc caacaggcgg tcgagcttcg cgaccagaac tcgctcggcc
     3061 ggcatccgag caactcgatc cagctcctcg ataagatcgt atcgaaggag cactgccgca
     3121 tcgagcgtcg cgccaacggc tacgtccttc gcgacctcgg cagcctgaac ggcacgtacg
     3181 tcaacggcga gcgcgtcaac ggcgagcaag atttgcggca cggcgacgat atcgcgctcg
     3241 gcagcactcg gggccggttc gacgacggta cgggtcgccc cgtcgcgccg cccgctcctt
     3301 acggctcccc tattgcctcg cccggcgcgc caccggcctg ggctccgccg ccgagcgtcg
     3361 gcacgccgca gagcctgaac gtcgcccaac ccgtgccgag ccacgtgcag gctccgccca
     3421 gcttgggtta cgccgcgccg tactctcagg ggcccacggc gcagttcgcg caaacgccag
     3481 ccgcgccgca tcagcccggc ggaccgctcg gaaccgtcgc gatgccgcag gtcggcggcc
     3541 ctggacagac tcgcgtcgga gtggatctga acgaccgctc gcg
//
LOCUS       DASTKS010000052         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_675771, whole genome shotgun sequence.
ACCESSION   DASTKS010000052 DASTKS010000000
VERSION     DASTKS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675771"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VFQ35_01140"
     CDS             <1..1341
                     /locus_tag="VFQ35_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HET9929253.1"
                     /translation="EQDQNELVRSLRPRVRNLVAAKPEKAVLSELPASPERVRVVYRR
                     AGDKYLLVEYGPLVLDLRLRFRVDALMSWIRQNELPGIIDLTPGIRSLQIHFDTAVLS
                     AERLLDALARAETELPALDSVEVPTRVVRLPLSWDDPATRLAIEKYTQTVRPDAPWCP
                     SNIEFIRRINGLESIDEVYRIVFGASYLVLGLGDVYLGAPVATPIDPRHRLVTTKYNP
                     ARTWTPENAVGIGGAYLCIYGMEGPGGYQFVGRTLQVYNRFRTTPLFSRDEPWLLRFF
                     DQIQFYPVSAAELLEMREAFLHGKVELDIRHDTFRLGDYERFLAENSTSIQSFKQKQQ
                     TAFEAERQRWVESGQLGFSESERTANGSERSDSGVPEGAQAVESHVPGSVWKLMVERG
                     ARVRKGDALVVVESMKMEVCVEAPEDGTVVELLVSEGKSVAPGQHIAFLRPEAR"
     gene            1338..3101
                     /gene="atzF"
                     /locus_tag="VFQ35_01145"
     CDS             1338..3101
                     /gene="atzF"
                     /locus_tag="VFQ35_01145"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HET9929254.1"
                     /translation="MTLVRHDPLRLAPAALVRDFRSGERDPREVLESIYAQIGRLGER
                     PVWISVQPFERALAALEAAERRGRNAPLFGVPFAVKDNIDVAGLPTTAACAEFSYVPD
                     ASAAVVERLVQAGAIVIGKTNLDQFATGLVGTRSPYGACSSVFDPRYISGGSSSGSAV
                     AVARGDVSFALGTDTAGSGRVPAAFNELVGLKPTRGLLSTRGVVPACRSLDCVSIFGR
                     TLDDAAFLLPLAAGYDAKDPFSRLAPTRPAALGGAFRIGVPSAPEFFGDTESERLFRA
                     SVAEHERRGATLVSVDMTPFHAAAALLYHGPWVAERLAAVGAFFERHEAALQPVLRGI
                     LNGAKRFSAKDAFEGSYRLAELRREIEPLWGQIDALLVPTAPSHYTHEEIEASPVELN
                     TRLGTYTNFVNLLDMCGLAVPAGRRDNGLPFGITYLAPAFEEARLFELARLARESNAS
                     LSHGAVGGIELAVAGAHLSGQPLNHELTSRGAKLLRTTRTAPTYRLFALATTPPKPGL
                     SYVGEKNGAAIEVEVWQLEAADFGSFVAGIPRPMGIGSTLLEDGSTVKGFICEPSSLE
                     GALDITSFGGWRSYRAATQRT"
BASE COUNT          537 a         1088 c         1040 g          553 t
ORIGIN      
        1 gagcaagatc agaacgagct cgtgcgctcg ctgcgtcctc gcgtgcggaa cctcgtcgcg
       61 gccaagcccg agaaggcggt gctctccgag ctccccgcga gccccgagcg ggtacgcgtc
      121 gtctaccggc gcgcgggcga caaatatctg ctcgtcgagt acggcccgtt ggtgctcgac
      181 ttgcgactcc gcttccgcgt cgacgcgctg atgagctgga ttcgccagaa cgagctcccc
      241 ggcatcatcg atttgacgcc cgggatccgc tcgctgcaaa tccacttcga tacggcggtg
      301 ctgtccgccg agcgcctgct cgacgcgctc gctcgtgccg agacggagct acccgcgctc
      361 gactcggtcg aggttccgac gcgcgtcgtg cgcttgccgc tctcgtggga cgatccggcg
      421 actcgcctcg cgatcgagaa gtacacccaa accgtgcgcc cggacgcgcc gtggtgtccg
      481 agcaacatcg agttcatccg tcgcatcaac gggctcgaat cgatcgacga ggtttaccgc
      541 atcgtatttg gagcaagcta cctggtgctg ggcctcggcg acgtgtactt gggggccccc
      601 gtcgctacgc ccatcgatcc gcgccaccgc ttggtgacca ccaaatacaa cccggcgcgc
      661 acgtggactc ccgaaaacgc ggtcgggatc ggcggcgcgt acctgtgcat ctacgggatg
      721 gaggggccgg gcggctacca gttcgtgggc cgaaccctcc aagtatataa ccgcttccgt
      781 acgacgccgc tcttcagccg cgacgagccc tggctcctcc gcttcttcga tcagatccag
      841 ttctatcccg tcagcgcggc ggagctcctc gaaatgcgcg aggctttctt gcacggaaaa
      901 gtcgagctcg acatccgcca cgacacgttt cggctcggcg attacgagcg cttcctcgcc
      961 gaaaactcga ccagcattca gagcttcaag cagaagcaac aaaccgcctt cgaggcggaa
     1021 cggcagcgct gggtcgagag cggacagctc ggcttctcgg agtccgagcg aaccgcgaac
     1081 ggcagcgaac gcagcgactc gggggtgcct gaaggagcgc aagcggtcga gtcgcacgtg
     1141 ccgggcagcg tctggaagtt gatggtcgaa cgcggagctc gcgttcgcaa aggcgacgcg
     1201 ctagtcgtgg tcgagtcgat gaaaatggag gtgtgcgtgg aggcgcccga agacggaacc
     1261 gtagtcgaac tcttggtgag cgagggcaag tcggtcgccc ccggccaaca catcgcgttc
     1321 ctgcgcccgg aggcgcgatg actttggttc gccatgaccc gctgcgactc gcccccgccg
     1381 ccttggtgcg cgactttcgc tccggcgagc gcgatccgcg cgaggtattg gaaagcatct
     1441 acgcgcaaat cgggaggctc ggagagcgcc ccgtctggat cagcgtgcag ccattcgagc
     1501 gtgccctcgc cgcgctcgag gccgcggagc gccgaggccg caacgcgccc ctctttggcg
     1561 tgcccttcgc ggtgaaggac aacatcgacg tcgcggggct tccgacgacg gccgcgtgcg
     1621 cggagttctc ctacgtgccc gacgcgagcg ctgccgtcgt ggagagactc gttcaggctg
     1681 gagcgatcgt catcggcaag acgaacctcg atcaattcgc gaccggcttg gtcggaacgc
     1741 gatcgcccta cggcgcctgc tcgtccgtgt tcgacccacg ctacatctcc ggggggtcga
     1801 gttcgggttc cgcagtggcg gtcgcccgcg gcgacgtgag cttcgcgctc ggaacggaca
     1861 ctgccgggtc gggccgagta ccggctgctt tcaacgagct cgtcgggctg aaaccgaccc
     1921 gaggcttgct gagcacgcgc ggcgtcgtgc cggcctgtcg ctcgctcgat tgcgtctcga
     1981 ttttcggccg aactctggac gacgcggcgt tcttgctacc gctcgccgcg ggttacgacg
     2041 caaaagatcc gttctcgcgc ctcgcaccga cgcgcccggc ggccttggga ggcgccttcc
     2101 gcatcggcgt accgagcgcg cccgagttct tcggcgacac ggagagcgaa cggctgtttc
     2161 gcgcatcagt agccgagcac gagcgccgcg gggcaacgct ggtctcggtc gacatgacgc
     2221 cgttccatgc cgcggctgcg ctgctctatc acggcccctg ggtggccgaa cgcttggcgg
     2281 ccgtcggcgc atttttcgag cgccatgaag ccgctctcca accggtgttg cgcggaatct
     2341 tgaacggggc gaagagattc agcgcgaagg acgccttcga ggggagctat cgtttggccg
     2401 agcttcgtcg cgaaatcgag ccgctttggg gccaaatcga cgcgctgctc gtgcccaccg
     2461 cgcccagcca ctacacccac gaagagattg aagcatcccc agtcgaactc aacacgcgcc
     2521 tcggcacgta cacgaacttc gtgaacctgc tcgacatgtg cgggctcgcg gtaccggctg
     2581 ggcggcgcga caacggcttg cctttcggca tcacttacct agcgcccgca tttgaagaag
     2641 cacgcctgtt cgaactcgct cgcctcgcac gggagtcgaa cgcatcgctt tcgcacggcg
     2701 cggtcggagg catcgagctc gcggtcgcgg gcgctcatct ctccgggcag cccctgaacc
     2761 acgagctcac gagccgcgga gccaagctgc tccgcacgac ccgcacggct cccacgtatc
     2821 ggctgttcgc gctcgccacc acgccgccca agccagggct cagctacgtc ggcgagaaga
     2881 atggcgccgc gatcgaagtc gaagtctggc agctcgaagc ggcggatttc ggcagtttcg
     2941 tcgctggcat cccgcggccg atgggcatcg gcagcactct gctcgaggac ggcagcaccg
     3001 taaaaggctt catttgcgag ccgagctcgc tcgaaggtgc gctcgacatc acgagcttcg
     3061 gcggttggcg aagctaccgc gccgcgactc agcgcacgta agactcatcc atggcgctcg
     3121 tgctcggtct cacgacggcg ttcgcatacg gaaccgccga cctcctcgct cgtgtttcta
     3181 cgcagcggct cggcgttttg agagctctgg tcgggacg
//
LOCUS       DASTKS010000053         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_454265, whole genome shotgun sequence.
ACCESSION   DASTKS010000053 DASTKS010000000
VERSION     DASTKS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454265"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFQ35_01150"
     CDS             <1..384
                     /locus_tag="VFQ35_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9929255.1"
                     /translation="LHVELPSDSITVHADRGAVSDALFNLLTNAYKYGGDPARIDVFA
                     EQKGSEVRITVRDNGQGIAPSEHKRIFQKFYRVDDRLSREREGSGLGLAIVKHVMRAH
                     RGRVELESTQGKGSAFTLVLPAMSR"
     gene            398..1096
                     /locus_tag="VFQ35_01155"
     CDS             398..1096
                     /locus_tag="VFQ35_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9929256.1"
                     /translation="MNGAAQKRILIVEDDESITLGLEMNLRAEGYDTVVAVDGDTGLA
                     RASEGGFDLIILDVMMPKMNGFEVLRALRERNKTVPVVMLSARGAELDKVMGLELGAE
                     DYITKPFGLAELLARVKAVLRRDGLLRQSASRVVRAGELEIKADAREVWRAGQLVDLT
                     ATEFDVLWCLVEAANLPLSREEILSRVWGEGHHGTLRTIDNFMLQLRNKLERDPTEPV
                     HLQTVRGVGYRFRA"
     gene            1214..1681
                     /locus_tag="VFQ35_01160"
     CDS             1214..1681
                     /locus_tag="VFQ35_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HET9929257.1"
                     /translation="MDSNLLRSYFATVYELPSDNGVVRVSMDGEAVRDIGTLPEILRK
                     DFAILTAYNPRSMLLPRRVNDVRHQVMRDLLIYGCYRVEHCVGYDDEPDGVWREPAWI
                     VHAIGRDEAIHFGRTFRQNTIVYCSAARPELVVTDPTCDDIGKTFQGNWRLRQ"
     gene            1731..2354
                     /locus_tag="VFQ35_01165"
     CDS             1731..2354
                     /locus_tag="VFQ35_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929258.1"
                     /translation="MVRVAASLLCLALPFVMVRQRVRAFAEGWATDTGRSLAALVPPM
                     PAPPPLSSADAAALAGFARAEVSALATPPPTPAAPQNQGKSRAKPQKPKEKAVFVPAS
                     TVLRLANGGATVPRAVYVPALGERPAGLRLLGVSALGVGMRDGDVLTRVLGAEVSSVG
                     EVVARVIAARNQRARSISGEFWRDGSRWALIVEQPYVDPEPAAPGGR"
     gene            2389..2673
                     /locus_tag="VFQ35_01170"
     CDS             2389..2673
                     /locus_tag="VFQ35_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929259.1"
                     /translation="MSRTLSLLNAGLFTLALALPAAAAPRDLGSNAPSCGGTDGTKKD
                     EKKNPSVADAQCDGPKTDGKGDKKNPSVADAQCDGPKTDGKGDKKNPSLG"
     gene            2721..2990
                     /locus_tag="VFQ35_01175"
     CDS             2721..2990
                     /locus_tag="VFQ35_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET9929260.1"
                     /translation="MAHMVQCVQLGREAEGLDRAPFKGDLGQRIYDSVSKEAWRRWLE
                     HSKMLINEYRMDLTSESGQRIWMTECEKYFFGEGSSAPSEYVPEK"
     gene            complement(3049..4185)
                     /locus_tag="VFQ35_01180"
     CDS             complement(3049..4185)
                     /locus_tag="VFQ35_01180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9929261.1"
                     /translation="MFWKKTSGEHKVGQPSSPAAALSSDDMALDTLASVVRAFGSGAF
                     DIDEVAAEQTRSECEAWAQRITVGEGRREGEGGGDFKRDWGGLRRYFSAHRTKEQDYV
                     GRSFMNLREAVQTFARCLAVAVGEDKTSDANVSQQLGKLVEAFRGNDPTIIRKEAENI
                     AAVVQAALDQRKQRQAEQFALLSNKIQKLRDELQHAREQATIDPLTQLHNRASLDGHL
                     ERVADLAFLLNSSPCLLMIDVDHFKSINDQYGHPMGDEVLRRIADCLVRQFLRREDFV
                     ARYGGEEFVVVIPDSTLQNAKKRAERVREAIEELAIECARGTLKVTISVGISSLAEGD
                     SGKSWLSRADAALYEAKASGRNAVQLANFPRHSFASLRPLSEKP"
BASE COUNT          746 a         1369 c         1414 g          773 t
ORIGIN      
        1 ctccacgtcg agctgccatc ggactccatc accgtgcacg ccgatcgcgg cgccgtctcg
       61 gacgcgctct tcaacctgct cacgaacgcc tacaagtacg gcggcgatcc ggcgcgcatc
      121 gacgtttttg ccgagcagaa aggctccgag gtgcgcatca cggtgcgcga caacggtcag
      181 ggcatcgcgc cgagcgagca caagcgcatc tttcaaaagt tttatcgcgt cgacgatcgc
      241 ctctcgcgcg agcgcgaagg ctcgggcctc ggtctcgcga tcgtcaaaca cgtgatgcgc
      301 gcgcaccggg ggcgcgtgga gctcgagagc actcaaggca aggggagcgc gttcacgctg
      361 gtgttgccag cgatgtcgcg ctgaggagca gcaatagatg aacggtgcgg cgcaaaaacg
      421 cattctcatc gtcgaagacg acgagagcat cacgcttggc ctggagatga acctgcgcgc
      481 ggagggttac gacaccgtcg tcgcggtcga cggtgacacg ggcctcgcgc gcgcttcgga
      541 ggggggcttc gacctcatca ttttggacgt gatgatgccg aagatgaacg gcttcgaagt
      601 gctgcgtgcg ctgcgtgagc gaaacaaaac cgtgccggtc gtcatgctga gcgcgcgcgg
      661 cgcggagctc gacaaggtga tgggcctcga gctcggagcg gaagactaca tcacgaagcc
      721 gttcggtctc gcagagctcc tcgcacgcgt caaagccgtg ctgcgtcggg atggcctcct
      781 gcggcaatcg gcgagtcgcg tcgtccgcgc tggcgagctc gaaatcaaag cggacgcgcg
      841 cgaagtttgg cgagccgggc agctcgtcga cctcacggcg accgagttcg acgtgctgtg
      901 gtgcctggtc gaagccgcga acctccccct ctcgcgtgag gaaatcttgt ctcgggtttg
      961 gggtgagggg caccacggga cgctccgcac gatcgacaat ttcatgctcc agctccggaa
     1021 caagctcgag cgggacccga ccgagccggt gcacctgcag acggtgcgcg gggtcggcta
     1081 tcgctttcgg gcgtagcaac agcgggcgaa gtcgagccgg ccgaagttgg cgggcgggac
     1141 gaaccgctaa aattccagcg ttcatgcgct ttcggtgcgg cttctacggg cgtgctataa
     1201 gcgccgccgc agcgtggact ccaacctact ccgctcctac ttcgcgaccg tttacgagct
     1261 tccctccgac aacggcgtgg tccgtgtttc gatggatggc gaagccgtgc gcgacatcgg
     1321 cactttgccc gagatcctgc ggaaggattt cgcaatcctg accgcgtaca acccacgcag
     1381 catgctgttg cctcgccggg taaacgacgt tcgtcaccaa gtgatgcggg accttttgat
     1441 ttacggctgc taccgcgtcg aacattgcgt tggatacgac gatgaacccg acggcgtctg
     1501 gcgcgagccc gcctggattg tgcacgccat cggacgtgac gaagcgattc actttggtcg
     1561 aacttttaga cagaacacca tcgtgtattg cagcgcggct cggcccgagc tggtcgtaac
     1621 ggatcccacc tgcgacgata tcggcaagac tttccaaggc aactggcgcc tccgccaatg
     1681 atcgtcggct cttagcgcgc gacgacgacg tgctacgatc agcgcccgca atggttcggg
     1741 tcgctgcgtc gctgctttgt ctggcgctgc cgttcgtgat ggttcggcag cgggtccgcg
     1801 cttttgcgga gggctgggcg acggataccg gccgcagctt ggctgctctc gtcccgccga
     1861 tgccagctcc gcctccgctt tcgtcagccg acgcggcggc tctagccggg ttcgcccgcg
     1921 ctgaggtttc ggcgctcgcg actcctccgc cgactcccgc tgctccccaa aaccagggga
     1981 aaagccgcgc aaagccccaa aagccgaaag agaaggcggt gttcgtcccg gcgagcacgg
     2041 tgcttcgcct tgccaatggg ggcgccaccg tgccgcgagc cgtctacgtc cccgcgctcg
     2101 gcgaacgccc cgcggggctg cggttgctcg gtgtttccgc gctcggcgtc gggatgcgcg
     2161 acggggacgt gctcacccgt gtgctcggcg ccgaggtgag ctcggtgggg gaagtcgtcg
     2221 cccgcgtcat cgccgcccgc aatcagcgcg cgcgaagcat ttcgggcgag ttctggcgcg
     2281 acggctctcg ttgggcgctc atcgtcgagc agccctacgt cgatccagag cctgcggcgc
     2341 ccggcggacg ttgacgacgg accggaacgg cctcagatct gccggaccat gagccgaacc
     2401 ctctcactgt tgaacgccgg tctcttcacg ctcgcgctgg ccttgccggc cgcggcggct
     2461 ccccgcgatc ttggcagcaa cgcgccgagt tgcggcggca cggacggaac gaagaaggac
     2521 gaaaagaaga acccgtcagt cgcggatgca cagtgcgacg gcccgaagac ggacggcaag
     2581 ggcgacaaga agaacccgtc agtcgcggac gcgcagtgcg acggcccgaa gacggacggc
     2641 aagggcgaca agaagaatcc gtcgctcggc tgacctcagc cggttgacgg cgcccttccg
     2701 gaacgtcaca ttcggcgccg atggcccaca tggttcagtg cgtgcagctc ggtcgcgaag
     2761 cggagggcct cgatcgggct ccgttcaagg gtgaccttgg gcagcggatt tatgacagcg
     2821 tctcgaagga ggcgtggcgt cgctggctcg agcactcgaa aatgctgatc aacgagtacc
     2881 gcatggatct cacgagtgag agcggtcagc gcatttggat gaccgagtgc gagaagtact
     2941 tcttcggaga gggctcgtcg gcgccgtccg aatacgttcc cgaaaagtga actcgaatac
     3001 gttcgcgaga aagcgagccc gcgcgcgctc gcgacgagtc ggaggagcct agggcttctc
     3061 cgacagcggc cgcagcgacg cgaacgagtg cctggggaaa ttcgcgagct gcaccgcgtt
     3121 gcgtccgctc gcctttgctt cgtagagcgc tgcgtcggcg cggctgagcc agctcttgcc
     3181 gctatcaccc tcggcgaggc tcgagatgcc gaccgagatc gtcactttca gagtgccgcg
     3241 agcgcactcg atggcgagct cttcgatcgc ttcgcgcacg cgctcggcgc gcttcttggc
     3301 gttctgtaac gtcgagtccg gaatcacgac cacgaactct tcgccgccgt agcgggccac
     3361 gaagtcttct cgccggagga actggcggac gagacagtcg gcgatgcgac gcagcacctc
     3421 gtcgcccatc gggtgcccgt actgatcatt gatgctcttg aagtggtcga cgtcgatcat
     3481 caagaggcaa gggctcgagt tcaacaagaa cgcgagatcg gcgacgcgct ccaagtggcc
     3541 gtcgagggac gctcggttgt ggagctgcgt gagcggatcg atggtggcct gctcacgggc
     3601 gtgctgaagt tcgtcgcgca gcttttggat tttgttactg agcagcgcaa actgctccgc
     3661 ttgccgttgc ttgcgctgat cgagcgcggc ttgcaccacg gccgcgatgt tctcggcctc
     3721 cttgcggatg atggtcggat cgttgccgcg aaatgcctcg acgagtttgc cgagctgctg
     3781 agagacgttc gcgtcgctcg tcttgtcttc gcccaccgcg acggcgaggc agcgcgcgaa
     3841 ggtctgcacg gcttctcgca ggttcatgaa gctgcgaccg acgtaatcct gctccttggt
     3901 ccggtgtgcc gaaaagtagc ggcgcagccc accccagtcg cgcttgaagt cgccgccccc
     3961 ttcgccctcg cggcgccctt cgcccacggt gatacgctgt gcccaagcct cgcactccga
     4021 gcgcgtctgc tccgccgcga cttcgtcgat gtcgaacgca ccgctgccga aggcgcgcac
     4081 gacgctagcc agcgtgtcga gcgccatgtc gtcgctcgag agggccgcgg ccggcgagga
     4141 cggctgacct accttgtgct cacccgaagt tttcttccag aacatgagcc ttccttttag
     4201 gaacggctgg aaccgggaga gcttcagttc tggtacggta actgacagct cgcagcactg
     4261 tggctcttag gatgacaggc ccctgtccgt tccgaatcct gc
//
LOCUS       DASTKS010000054         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1346543, whole genome shotgun sequence.
ACCESSION   DASTKS010000054 DASTKS010000000
VERSION     DASTKS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346543"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFQ35_01185"
     CDS             complement(<1..189)
                     /locus_tag="VFQ35_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929262.1"
                     /translation="MRLDVKAGWSLIKEAAEDWDADDASSLAAALACYTLLSIAPLTV
                     VAVSIGGMLFGKEAARGQI"
     gene            448..1344
                     /locus_tag="VFQ35_01190"
     CDS             448..1344
                     /locus_tag="VFQ35_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929263.1"
                     /translation="MTVARDTLVEYIQNPSTRSRLHTYMRRRGLADSADDLVQTVLCD
                     ALALTVEAVPVEPSELPRFVTGIARNKAADEHRRRARWNRKGDAEALEAVDGATTPDG
                     AAGFTPCASPTHEARDLLQRIERDFNQPREREALSCLLREHAGESLYELARERALEPD
                     TLRQRICRLRKELRARYLAPLALAVAFGVVGLNAWVRHVDTIALGVGSPLAAYAGEWR
                     LTHVEPASYAAMHLRVRIDARAIHVYSAAEPLGRELYVERIDGKHIRLRCGDRHWDGT
                     LRELSSRRIELRTARGAVVLER"
     gene            complement(1360..>2204)
                     /locus_tag="VFQ35_01195"
     CDS             complement(1360..>2204)
                     /locus_tag="VFQ35_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HET9929264.1"
                     /translation="IADDKDDAAGGIAVFDMQGKLLQLRKDGKIGNVDLRAGFPLGGK
                     EIVLVGANNRTNGSIEFWQLDTEARRLSAPLGESPTTNLPNYGFCLYHSAKSGKFYAF
                     VTQETGASTLEQYELSESSGKVSAKRVRSFDVGSITEGCVADDELGRLYVAQEDVALW
                     RYGAEPESGSERTQVAAVGDGHLVADLEGVGLAKGPGTSGYLVVSIQAENRFATYDRE
                     TNAYLRGFSVAANGAIDAVNQTDGLDISTENLGPGFPKGALVVHDGSNTNGDTSNLKF
                     VPLD"
BASE COUNT          379 a          745 c          695 g          385 t
ORIGIN      
        1 gatttgcccg cgcgccgcct ccttcccgaa gagcatgccc ccgattgaaa ccgccaccac
       61 cgtcagcggc gcaatcgaga gcagggtgta acacgcgagc gctgccgcga ggctcgaggc
      121 atcgtccgca tcccagtcct cggccgcctc tttgatcagc gaccaaccgg ctttgacatc
      181 gaggcgcatc caagacgccg attcaaaaag cgcgccagtg gtacatcgac gtgcgccccg
      241 cgttcgcagc tcggcgcccg catcgcgaac gcgccacgct gaggcagacg agcggcggct
      301 cgaggaccgt gttccaagtg aatcgcgcag ccggcaagca cgtccaaagc ggggtgggcg
      361 gtgcgtcatg aagattttct tggttttttg gtcacgcgcc ggcgcctcca gcgataagca
      421 ggaaagaagc ccccaaggag acctgacgtg accgtggctc gggacaccct cgtcgagtac
      481 atccaaaacc ccagcacccg ctcgcggctc cacacctaca tgcgtcggcg gggcctggcc
      541 gacagcgcgg acgacctcgt tcaaaccgtg ctgtgcgacg cgctcgcgct gacggtcgag
      601 gctgtccctg tggaacccag cgagttgccg cgcttcgtta ctggcatcgc tcgcaacaag
      661 gcggctgacg agcaccgccg ccgcgcgcgc tggaaccgaa aaggcgatgc cgaagcgctc
      721 gaggcagtcg atggtgccac gacaccggac ggcgccgcgg gctttactcc atgcgcgtcg
      781 cctacgcacg aagcgagaga tctgctgcag cgcatcgaac gcgacttcaa tcaaccgagg
      841 gagcgcgaag ccctcagctg cctcctccga gaacacgcgg gggaatctct ttacgagctc
      901 gcgcgcgaac gcgcactcga gccggacacg ctgcgccagc gcatttgccg actgcgaaag
      961 gagctccgcg ctcgctactt ggctccgctc gcgctcgcgg tcgcgttcgg cgtggtcggg
     1021 ctcaacgcgt gggtgcggca cgtcgacacc atcgcgctgg gcgttggctc accgctcgca
     1081 gcctacgccg gcgagtggcg cctcactcac gtcgagccgg cgagctacgc cgccatgcac
     1141 ttgcgcgtgc gcatcgacgc gcgagccatc cacgtttaca gcgccgccga gcccctcggt
     1201 cgcgaactct acgtcgagcg catcgacggc aagcacattc gcctccgctg cggagacagg
     1261 cactgggacg gcacgctgcg agagctctcg tcgcgacgca tcgagctcag aacggcgcgc
     1321 ggcgcagtcg tgctcgaacg ctgaacgacc ggtgctcgtt cagtcgagcg ggacgaattt
     1381 gagattcgag gtatcgccgt tcgtattgct tccgtcgtgc acgacgagcg cgcccttggg
     1441 gaagccgggg ccgaggtttt cggtcgagat gtcgaggccg tcggtttggt tcaccgcgtc
     1501 gatcgcgccg ttcgcagcga cgctgaaccc tcgcaggtaa gcgttcgtct cgcgatcgta
     1561 ggtcgcgaag cgattttccg cttggatcga gacgaccaag tagccgctcg tgccaggtcc
     1621 cttggcgagg ccgactccct cgagatcggc aacgaggtga ccatcgccca ccgccgcgac
     1681 ttgcgttcgc tccgagccgc tctctggctc ggcaccgtaa cgccagagcg cgacgtcttc
     1741 ctgcgcgacg tacaggcgcc ctagctcgtc gtctgcgacg catccctcgg tgatgctccc
     1801 cacgtcgaac gagcgcacgc gcttagccga aactttgccg ctgctctcgc tgagctcgta
     1861 ttgctccagc gtcgaagcgc cggtctcttg cgtgacgaag gcgtagaact tgccgctctt
     1921 cgcgctgtga tagaggcaaa agccgtaatt gggcaggttg gtggtggggc tctctccgag
     1981 cggtgcggac aagcgccgtg cttcggtatc gagctgccag aactcgatgc tgccgttcgt
     2041 gcgattgttc gcgcccacga gcacaatttc tttgccgccc aaggggaagc cggcgcgcaa
     2101 atcgacgttg ccgattttgc cgtctttgcg aagctgcaag agctttcctt gcatgtcgaa
     2161 cacggcaatt ccgccggccg cgtcgtcttt gtcgtcggcg atta
//
LOCUS       DASTKS010000055         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_8394,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000055 DASTKS010000000
VERSION     DASTKS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8394"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1464
                     /locus_tag="VFQ35_01200"
     CDS             235..1464
                     /locus_tag="VFQ35_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929265.1"
                     /translation="MLARAEELSSAGLSEDAARLSESAGDRLLSLGNSLGAAVAYQRA
                     LHHFPVEVAARSRKHHISERLSGALSELGMASGMNRERRIESLFRAAARYWSDQPAGG
                     HRAVCFECGELKPAGWIRCSSCAAVPKLSEQVAFGLLASERACSSERLREIGRRLQQG
                     DSPIPPDVQRLFATALYQCSAEREDAPGSLPPGTVVAVPSSEGRAAVLLEVSTSGEHV
                     SEKPECVELIEQVCARLLTSPDSVTLEDIRLLDGRNVAEAGGSVSRRLGSRIDFPRWR
                     RIEVPRGAVGVAVVGATISRPRDSRPHRVRACGNLAAAAVALHANSDGTEMVFDLAGQ
                     LAHQLVRAHVVDSEHAELAGERFALDPSLTVADALGVLSKALEHDVRLGVAACYVRHP
                     FADDEPPKFPGEAALRA"
     gene            complement(1516..2496)
                     /locus_tag="VFQ35_01205"
     CDS             complement(1516..2496)
                     /locus_tag="VFQ35_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929266.1"
                     /translation="MTDPATPLATEAELLGDPESPLSQAVRHFRRDRVSMTALWVLAL
                     QVLVALLGKFFTSGWVLFDPSVVRLVDKLRPPLSAPTRALLRPDQMPFFGVYLLGTDE
                     LGRDVFARMLQGGFVSLSIGILAVSISVTIGILLGGTAGYYGQRKLAFVSIDTLIMRF
                     TDVVLCFPTFFLILTVVALLPPSIFNVMIVIGATSWMSTARFVRAEFLAQREQDYVVA
                     AHALGLSERRIIFRHLVPNAMAPVFVSATIDVAFAVLTESALSFLGFGVQPPHATWGN
                     ILADGRVYIFDAPWLFLTPGVAILTLVLAFNLVGEGLREAMSPKLRLRSS"
     gene            complement(2493..>3107)
                     /locus_tag="VFQ35_01210"
     CDS             complement(2493..>3107)
                     /locus_tag="VFQ35_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929267.1"
                     /translation="GIHAAVKRGGVFDRTSTVIAYALISIPNFFLSYLVILAVVRVFQ
                     LPVLGLHTFGTARESLFVRGMDSVWHLAVPSVMSALTGIAVLSRYVRSQMVEVLGQDY
                     VRTARAKGLGEDEVVYGHALRNGLLPFVTMFGFLLPGLIGGSVIFENIFAWPGLGRLG
                     YDSIMARDFPMILTLNFIAAVLTLLGVLVSDILYALVDPRIRLE"
BASE COUNT          578 a          979 c         1051 g          499 t
ORIGIN      
        1 gcatggccgg ctacttcgtg gcggccttcg accaggaggt gcgagctctt caagaccacg
       61 tggccggtac tcgcgtcgtg cagaatgcat gatttttagc agcggcggcc gggcgttccg
      121 aagcgtttcc actgcgcttc caagcaggta aacaggcttc tcgcgttcgc gttcgacaac
      181 gccgggtcgc cgtggaattc tctcgaattc atgctggatg ccgaggcgaa tcagatgctc
      241 gcacgcgctg aggagctgtc ttccgctggg ctctccgagg acgccgcgcg cttgagcgag
      301 tcggccggag atcgcctgct ctcactcgga aactcgctcg gagccgcggt cgcttatcag
      361 cgcgcgctcc atcattttcc cgtcgaggtc gcggcgcgtt cgcgaaagca ccacatctcg
      421 gagcggctgt cgggtgcgct cagtgagctc ggcatggcaa gtggcatgaa ccgcgaacgg
      481 cgcatcgagt cgctattccg tgccgcggcc cgctattggt ccgatcaacc cgcagggggg
      541 caccgcgccg tgtgcttcga gtgcggcgag ttgaagcccg cggggtggat tcggtgctcg
      601 agctgtgctg cggttccgaa gttgtccgaa caggtcgcgt tcggtttgct cgcctcggag
      661 cgcgcctgtt cgagcgagcg cctgcgcgaa atcgggcggc gcctgcagca gggagattcg
      721 ccgattcccc cagacgtgca gcggctcttc gcgacggcgc tgtaccaatg ctccgccgag
      781 cgagaggacg ctcccggttc gttgccaccg ggaaccgtcg tcgccgttcc gagcagcgag
      841 gggcgcgcgg ccgtgctgct cgaggtgagc accagcggcg agcacgtgag cgaaaagccc
      901 gagtgcgtcg agctcatcga gcaggtctgc gcgcgtctcc tgacgagccc cgattcggtc
      961 acgctcgagg atattcgtct gctcgacggg cgcaacgtag cggaagcggg cggctccgtg
     1021 tcgcggcgcc tcggctcgcg catcgatttt ccgcgttggc gccgcatcga ggtcccgcgc
     1081 ggtgcggtgg gggtcgctgt ggtcggggcc accatttcga ggcctcgcga cagtcgtccg
     1141 catcgggtgc gagcgtgcgg caatttggcg gctgccgccg tcgcgctcca cgccaattcg
     1201 gacggtaccg agatggtctt cgatttggcg ggacagctcg cgcaccagct cgtgcgtgct
     1261 cacgtcgtcg attcggagca cgcggagctt gccggtgagc gctttgcgtt ggacccgtcg
     1321 ttgaccgtcg ccgacgcgct cggcgtgttg agcaaagcgt tggagcacga cgtgcggctc
     1381 ggcgtcgcgg cctgctacgt acgtcaccca ttcgccgatg acgagccgcc gaagttcccc
     1441 ggcgaggccg cgcttcgcgc ttgagcgatg ctcgccgcga cgtcgtgtcg cgaacgtagc
     1501 ccggcccttg gcgtattagc tcgagcgcaa acgaagcttg gggctcatcg cttcgcgcag
     1561 accttcgccg accaggttga aggcgagcac cagggtgagg atggcgacgc ccggggtgag
     1621 gaagagccag ggcgcgtcga agatgtaaac gcggccgtcg gcgaggatgt tgccccacgt
     1681 cgcgtgcggc ggttgcaccc cgaagcccaa aaaggagagc gccgactcgg tcaacaccgc
     1741 gaaggcaacg tcgatggtcg cggacacgaa caccggcgcc atcgcgttcg gcacgagatg
     1801 cctgaagatg atgcgccgtt cggataaacc cagcgcgtgc gccgcgacga cgtagtcttg
     1861 ctcccgctga gcgaggaact cggcgcggac gaagcgcgcg gtgctcatcc aactggtcgc
     1921 accgatcacg atcatcacgt tgaaaatgct gggcggcagc agcgccacga ccgtcaagat
     1981 caagaagaac gtggggaagc aaagcaccac gtcggtgaag cgcatgatga gcgtgtcgat
     2041 gctgacgaac gcgagcttgc gttgcccgta gtaccccgcc gttcccccca gcaagatgcc
     2101 gatcgttacg gagatgctga cggcgaggat gccgatcgag agcgaaacga agccgccctg
     2161 cagcatgcga gcaaagacgt cacgaccaag ctcgtccgtc ccgagcaggt acactccgaa
     2221 gaacggcatt tggtcgggcc gcaagagcgc gcgcgtcggc gccgacagcg gcggtcgcag
     2281 cttgtccacg aggcgcacga cgctgggatc gaagagcacc caaccggagg tgaagaattt
     2341 gcccagcaac gccaccaaga cttgaagtgc gagcacccaa agcgcggtca tcgacacgcg
     2401 atcacgccga aagtggcgca ccgcctggga aagcgggctc tcggggtcgc cgagtagctc
     2461 ggcttctgtc gcgaggggcg ttgcggggtc tgtcattcga ggcgaatgcg cgggtcgacc
     2521 agcgcgtaca gaatgtccga gacgagcacg ccgagcaggg tgaggacggc ggcgatgaaa
     2581 ttgagcgtca agatcatggg aaagtcgcgc gccatgatcg agtcgtagcc gagccgtccg
     2641 agcccgggcc aggcgaagat attttcgaag atgaccgaac cgccgatcaa ccccggaagc
     2701 aagaagccga acatcgtgac gaaggggagc aggccgtttc gcagcgcgtg gccgtacacg
     2761 acctcgtctt cgcccaagcc cttggctcgc gcggtgcgca cgtagtcttg ccccagcacc
     2821 tcgaccattt gcgagcgcac gtagcgcgac aacaccgcga ttccggtcag cgccgacatc
     2881 accgacggaa ccgcgaggtg ccagaccgaa tccatgccgc gcacgaagag cgactcacgc
     2941 gcggtgccga acgtgtgcaa ccccagaacc ggtagctgaa aaacgcgcac caccgccaaa
     3001 atcacgaggt acgacaaaaa gaagttcggg atggaaatga gcgcgtacgc gatgaccgtg
     3061 ctcgtgcgat cgaagacgcc acctcgcttc accgccgcgt gaatgcc
//
LOCUS       DASTKS010000056         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_676610, whole genome shotgun sequence.
ACCESSION   DASTKS010000056 DASTKS010000000
VERSION     DASTKS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676610"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2338
                     /locus_tag="VFQ35_01215"
     CDS             <1..2338
                     /locus_tag="VFQ35_01215"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HET9929268.1"
                     /translation="ARFRDERSDFIGLLKLWDFVREQDSRSTSQLRRACKEGFLSFIR
                     VREWREGYRQLEDLVREYRVVGGRSRADTPNRVEAPRNQRPQAQSKNAPRADPAEAIH
                     RALLTGLLSRVGQWNSEQRVYIGARQTRFALHPSSGLAKKPPPWVMAFELVETSQLFA
                     RTVAKVEPAWLAEVGAHLLKRSYSDPHWSEKSARASVREHSTLFGLTVVRDQSVDYAS
                     ISPVRARLMFIEHALVRGEYKSRGSFQEKNQRLLEEAARLRDKARQSDMLTDDEVLLE
                     FFDRRIPESVVNGKTFEDWREKAERANPNLLCFSLSHVLAREPGLSPERYPDEIIVRG
                     TALQALYRFDPSAADDGVTLNIPLAVLPQLSPEDLDGTIPAWREQKIEALLEALPRAL
                     RKDLGSVSELAARFCAALRSDEGSLLDALSRLVSLEAGVSVLPDVFRTDAIAAHLRFN
                     FRILDERGEVVEESRNLEELLHRHAGRARDAVRRAAPRTEIERAGITSWDFGDLPRSV
                     TRRVLGTELTSFVALADCQTSVAVQLFDTEAAAAAAHREGVRRLLQLATKGALASLAK
                     RVPPPFLRRVGLPSPRAEADAFRDAVLARVISETFELSEVALLPRSKAEFDTLLSAGT
                     SRLPRVFDAVVRLIAEVDTELHTTLRALDDAAKQKNGGGAVPDIRAQIEQLFPAELMQ
                     RIELLRLQQYPRYLRAARARLTRAVNDPRKDAAKAAPFAPIWQTFLAKRAKARDPRAA
                     ERVHYVLEELRVALFAPELKPAQPVTVATAALAVQSLQ"
     gene            2394..3113
                     /locus_tag="VFQ35_01220"
     CDS             2394..3113
                     /locus_tag="VFQ35_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929269.1"
                     /translation="MLPARTNLLFLRRSLGEAVADDYVAWALERLTAGADTPSLRILA
                     GLNTRLESDDVEPYFRKTCDELALAPPPVETSVREAVSLVRAAYESSELSPRETLEVV
                     ADIYHWDCRENAALRFLASMSAELPLAGSGGDGSCYPPTALNPLDAAVKREWALYDRY
                     ISLELPPAFDVFVMCEACHHVGEVSWRNNPLWKQLVTRVPGAQACCAVCGATRFRHMS
                     DPAVREEYFTRLEAARATERG"
     gene            complement(3122..>3496)
                     /locus_tag="VFQ35_01225"
     CDS             complement(3122..>3496)
                     /locus_tag="VFQ35_01225"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HET9929270.1"
                     /translation="VDEEPRRHRDDPDAPWPVRHGGLALGMYRHSLSAALFGLFALSF
                     ALHAFGGLRAANTSRALHGLSAQTAWEFVSSAEFWFQSFQNWQSEFLSVMSLVLLSIV
                     LREFGSPQSKPVAAPHAETGGA"
BASE COUNT          598 a         1144 c         1136 g          618 t
ORIGIN      
        1 cgcgcgcttt cgcgacgagc gctctgactt catcggcttg ctcaagctgt gggatttcgt
       61 gcgagagcag gacagccgga gcacttcgca gctgcggcgc gcctgcaaag aaggattcct
      121 ctcgttcatt cgggtgcgag agtggcgcga aggctaccgg cagctcgaag atctcgttcg
      181 ggagtatcgc gtggttggcg ggagaagccg cgccgacacg ccgaaccgcg tcgaggctcc
      241 gcgaaaccaa cgaccacaag cgcagtcgaa gaatgcaccg cgcgccgatc cggcggaagc
      301 gattcatcgc gctttgctga caggcttgct gtcgcgcgtg ggccagtgga actcggagca
      361 gcgtgtctac atcggtgctc gccagactcg gttcgcgctg catccttcct cgggtctcgc
      421 gaagaagccg ccgccgtggg tgatggcgtt cgagctcgtc gaaacatctc agctcttcgc
      481 tcgcacggtc gcaaaagtag agccggcctg gctcgccgag gtgggcgccc atctcctcaa
      541 aaggagctac tcggatccgc actggtccga gaagtcggcg cgcgcgtccg tccgtgagca
      601 ctccactctt ttcggtctca ccgtggtgcg cgatcagagc gtcgactacg cgagtatttc
      661 gcccgtgcgc gcgcgcctca tgttcatcga acatgcgctc gtgcgcggtg agtacaagag
      721 ccgcggctcg ttccaggaga agaatcagcg cctgctcgaa gaggccgcgc gtctccgcga
      781 caaagctcgt cagagcgaca tgctcaccga cgacgaggtg ttgctcgaat ttttcgatcg
      841 gcggatcccc gaaagcgtgg tcaacggaaa gaccttcgag gactggcgcg aaaaggccga
      901 gcgcgcgaac ccgaacctgc tttgcttctc tctctcgcac gttcttgctc gcgagccagg
      961 gctctccccg gagcgctacc cggacgagat catcgttcgc ggcaccgcgc ttcaggccct
     1021 gtaccgcttc gatccgtcgg cggcggacga cggcgtcacc ttgaacatcc cgctcgcggt
     1081 cttgccccag ctttcgccgg aagatctgga cggtacgata ccggcgtggc gcgagcagaa
     1141 gattgaggcg ctgctcgaag cgttacctcg tgcgttgcgt aaagatctgg gttcggtttc
     1201 agagctcgcg gctcggttct gcgccgcgtt gcggagtgac gaaggctcgc ttctcgacgc
     1261 gctgagccgc ctcgtttcac tcgaggccgg cgtcagcgtt ctgcccgacg tttttcgcac
     1321 cgatgcgatc gcggctcatc tgcggttcaa cttccgcatt ctcgacgagc gcggcgaagt
     1381 cgtcgaggaa agccgaaacc tcgaagagct cttgcatcga cacgccggac gggcgcgcga
     1441 tgcggttcgg cgcgcggcgc cgcgaacgga gatcgaacgc gcgggcatca cgtcgtggga
     1501 cttcggagac ctcccgcgca gcgtgacgcg gcgggtgctc ggtactgagc tgacgtcgtt
     1561 cgtcgcgctc gccgattgtc aaacgtcggt cgccgtccaa ctcttcgaca ccgaagcggc
     1621 ggctgcggca gctcatcgag agggggttcg ccgtttgctc cagctagcga cgaagggagc
     1681 cctcgcgtct ttggcgaagc gcgttccccc gccgttcttg cgtcgagtcg gtttgccctc
     1741 accgcgcgcg gaggcggacg cattccgcga cgccgtgctc gctcgtgtca tcagcgagac
     1801 cttcgagctc tcggaagtgg ctttgctccc gcgctccaaa gccgaattcg acacgctgct
     1861 ctctgcgggc acctcgcggc tgccgcgcgt tttcgacgcg gtggtgcggc tcatcgccga
     1921 ggtcgacacc gagctgcaca ctacgctccg cgcgctcgac gacgcggcga aacaaaagaa
     1981 cggcggcggc gcggtcccag acatccgcgc gcaaatcgag cagctctttc ctgcagagct
     2041 catgcaacgc atcgagctct tgcgtctgca gcagtatcct cgctacctgc gcgcggcgcg
     2101 agcgaggctg actcgcgccg tgaacgaccc ccgcaaagac gccgccaaag ccgcgccgtt
     2161 cgcgcccatt tggcagacgt tcctcgccaa aagagccaaa gcgcgcgacc cgcgcgcggc
     2221 ggagcgcgtt cactacgtgc tggaagagct tcgggtagcg ctctttgctc ccgagttgaa
     2281 gccggctcag ccagtgacgg tcgccactgc agcgctcgcc gtccagtcgc tgcaatgaat
     2341 cgcgcgagcg ctgcacggcg cggtcgccga tttgctaaga tgaggggcgc accgtgcttc
     2401 ccgccagaac gaacctactg tttcttcgtc gcagtctggg cgaggccgtg gcggacgact
     2461 acgtggcgtg ggcgctcgag cgactgacgg ccggcgcgga cactccgagc ctgcgcatct
     2521 tggcggggtt gaacacgcgg ctcgagtcgg atgacgtcga gccgtacttc agaaagacgt
     2581 gcgacgagct cgcgctcgcg ccgccgcccg tcgaaaccag tgtgcgcgaa gccgtctcgc
     2641 tcgtccgggc tgcctacgaa agcagcgagc tttcgccgcg cgaaacgctg gaggtggtgg
     2701 ccgacattta ccactgggac tgccgagaga atgccgctct gcgtttcttg gcgagcatga
     2761 gcgccgagct cccgctcgca gggtctggag gcgatgggtc ctgctatccg ccgaccgcgc
     2821 tcaatccgct agacgcggcg gtgaagcgcg aatgggctct ctacgaccgc tacatttcgc
     2881 tcgagctgcc gcccgccttc gatgttttcg tgatgtgtga ggcctgtcat cacgtgggcg
     2941 aggtgagttg gcggaacaat ccgctatgga agcagctcgt cacgcgagtg ccgggcgcgc
     3001 aggcctgctg cgccgtatgt ggcgcgaccc gctttcgcca catgagtgat cccgcggttc
     3061 gcgaagagta tttcactcgc ctcgaggcgg cgcgagcgac cgagcgcggg taagctcgag
     3121 ttcaagcccc accagtttcg gcgtgcggcg ctgccaccgg cttcgactgc ggcgacccga
     3181 actcgcgcag aacgatcgag agcagcacga ggctcatcac cgacaagaac tcgctttgcc
     3241 agttctggaa cgactgaaac cagaactcgg cggagctcac gaactcccac gccgtttgcg
     3301 cagagagacc gtgcaacgcc cggctcgtat tcgcggcgcg caggccaccg aaggcatgca
     3361 gagcgaagct caaggcgaac aacccgaaga gcgcggccga aagtgaatga cggtacatac
     3421 ccagagcgag tccgccatgt cgcacgggcc agggcgcgtc ggggtcgtcg cgatgtctgc
     3481 gcggctcttc gtccac
//
LOCUS       DASTKS010000057         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_454522, whole genome shotgun sequence.
ACCESSION   DASTKS010000057 DASTKS010000000
VERSION     DASTKS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454522"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(529..2454)
                     /locus_tag="VFQ35_01230"
     CDS             complement(529..2454)
                     /locus_tag="VFQ35_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9929271.1"
                     /translation="MSLSSSKLERLGGGALFEALRIAADRSKLGLALIFVDREPPEVL
                     LLNEYASELLGYSPEEVLKLGPWKLVAPEYVPDTRGPNAEWSRTVAGSQVEAVIVTKQ
                     GKHVPVEVTLSDVVIDARRAFIVFFRDLSERWRAINALQESEERFRLVVEGAPDGVAI
                     LNGPRIRFLNPRAARMLGVADSESAEGRLITEFLHPEDREAAAQRIRQLLQTGKAHRD
                     AAEYRSRSLEGDELVVEISAIAIEIGGERMVLAFARDVTERKAIQRRLAEAERLTALG
                     VLSAGVAHEINNPLAYVLLNLEYLRRELPRLATQPERLEDLMIRVRDACHGAERVATI
                     VRDLRTFARGDDSVRGPVDLQEVLEAAVNIAGNTLSQRARVVRDYSDAPPVEGNPNRL
                     EQVFLNLLLNAAQAMPNSDPSRDEIRIRIRHGNGRVWVDVEDTGEGIPDEILSRIFEP
                     FFSTKPVGVGTGLGLPICRSIIASHNGSISVESEPGKGATFRVELPASKQPRETGSNR
                     PSGSPSPDAPRGRILIVDDEVAVGSTLRLVLQGEHDAHVATSAAEALQMLSQVAYDAI
                     VCDVAMPGMTGMDLYDAIRVAYPGTESSMIFMTGGTLVPQSQEFLSKLENPLLEKPFD
                     TELLRQTLRRLVRRRRR"
BASE COUNT          445 a          761 c          960 g          492 t
ORIGIN      
        1 gggtagcgtg cgctggcgct agctggaagc ggggcgtggg agcgtgcgct ggcgccagtt
       61 ggcagccggg cgtcggcagc ccgcgccaag gggagtgggc tgcgccatcg taaggggagg
      121 gtcacgaccg aagacgtcga gctcggcaat gggtgcgcga tcatgagtac gtatacttac
      181 tctggtcgcg agatcgaact ttcatgattt cagatgctta cagcgccgcc cagagggtca
      241 accttgaatc gtcggcgctt cgcatcggag cgaaatctga agcagcacaa actccgcggt
      301 ttggctcatt gggataaact atgcatacat acatgaagtt gggcgacagg gagggttgga
      361 actagtccga tttcccggac acttggcgtc gcaggtggtg cgtcgcggct ttccgcaccg
      421 gcgctgggcg agtgagcgac gctggccttc ggagtgcgtg gagtgcgtgg agcgcgtggt
      481 gcgcttcgtg ggatcgggac gggggagtga gcgggagcgg gggtggggtt agcgtctccg
      541 acggcggact aggcgtcgga gggtttggcg cagtagttcc gtgtcgaagg gcttttcgag
      601 gagggggttc tcgagcttgg agagaaattc ctggctttgg gggaccagag tgccgccggt
      661 catgaagatc atggacgact cggtgccggg gtaggcgacg cggatggcgt cgtagagatc
      721 catgccggtc attccgggca tcgcgacgtc gcagacgatg gcgtcgtagg cgacttgcga
      781 aagcatttgg agggcttcgg cggcgctggt ggcgacgtgc gcgtcgtgct caccttggag
      841 tacgaggcgt agggtgctgc cgacggcgac ttcgtcgtcg actatcagga tgcggccgcg
      901 gggcgcatcc ggcgaggggg acccggaagg gcgattggag ccggtttcgc gcggttgttt
      961 ggaggcaggg agctcgacgc ggaaggttgc gcctttgcca ggctcgctct cgacggagat
     1021 ggaaccgttg tggcttgcga tgatgctgcg gcaaatgggg aggccgaggc ccgtaccgac
     1081 gccgaccggc ttggtcgaaa agaagggctc gaagatgcgc gagaggattt cgtcggggat
     1141 gccctcgccg gtgtcttcga cgtcgaccca gacgcggccg ttgccgtgac ggatccgaat
     1201 gcggatctcg tcgcgcgagg ggtcgctgtt cggcatggcc tgggcggcgt tcaggagcag
     1261 gttgaggaag acttgttcga ggcggttcgg gttgccttcg accggcggcg cgtcgctgta
     1321 atcgcgcacg acgcgcgcgc gttgactcaa ggtgttgccg gcgatgttca cggccgcctc
     1381 caggacctct tggaggtcga cggggccgcg cacgctgtca tcgccgcgcg cgaaggttcg
     1441 gagatctcga acgatggtcg cgacgcgctc cgcgccgtgg caggcgtcgc gcacgcgaat
     1501 catcaaatct tcgaggcgct cgggctgcgt tgccagacgc ggcaactcgc gacgcagata
     1561 ctcgaggttc aagagcacgt aagcgagcgg attgttgatc tcgtgcgcta cacccgccga
     1621 aagtacgccg agcgccgtga gccgctcggc ctcggcgagg cgccgttgga tggccttgcg
     1681 ctcggtgacg tcgcgcgcaa aggcgagcac catgcgctca ccgccgattt cgatcgcgat
     1741 ggccgaaatc tcgacgacga gctcgtcacc ctccagggag cgggagcggt actccgccgc
     1801 gtcgcgatgc gccttcccgg tttggagtaa ctgacggatg cgttgcgcgg ccgcttctct
     1861 atcttcgggg tgaagaaact cggtgatgag acggccttcg gcgctctccg aatccgccac
     1921 ccccaacatg cgcgccgcgc gggggttcaa gaaacgaatt cgcggaccgt tcaagatggc
     1981 gacgccgtcg ggggcgcctt caaccaccaa acgaaagcgc tcttcggact cttgcaaagc
     2041 gttgatcgcg cgccagcgct cgctgagatc cctgaaaaat acgatgaacg cgcggcgcgc
     2101 gtcgatgacc acgtcgctca gcgtcacctc caccggcacg tgcttgcctt gcttcgtgac
     2161 gattaccgcc tcgacttggc tgcccgcgac ggttcgactc cactccgcgt tcggaccgcg
     2221 cgtatcgggc acgtactcgg gcgcgacgag cttccacggc ccgagcttga gcacctcctc
     2281 cgggctgtag ccgaggagct cggaggcgta ctcgttgagg agcagcacct ctggcggttc
     2341 tcgatccacg aagatgagcg cgagacccag cttgctgcga tcggccgcta tgcgtagggc
     2401 ctcgaagaga gcgcctcccc caagtcgctc gagctttgag gacgacaaag acattcggca
     2461 ccgccgaggg tacaccgact ctctccgcag gggcagcgat tgcggtttcg agtggtgacg
     2521 cgcgcaggtg atctcgcggg cgtgagccgg gcatcgtggc cgcgaatttc ggcaaatttg
     2581 gccagttggt ccggcgtgtg cgacgtgctg actcatggcg aacacactgc gcgttctggc
     2641 tggaatgtta ttgctgcc
//
LOCUS       DASTKS010000058         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_899826, whole genome shotgun sequence.
ACCESSION   DASTKS010000058 DASTKS010000000
VERSION     DASTKS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899826"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFQ35_01235"
     CDS             complement(<1..189)
                     /locus_tag="VFQ35_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929272.1"
                     /translation="MTSTATAHGQRGRDTASSTGFSAANVAGGGDELEVLSEVELGAS
                     AASTLVGGVTFGGSACAGP"
     gene            complement(477..2237)
                     /locus_tag="VFQ35_01240"
     CDS             complement(477..2237)
                     /locus_tag="VFQ35_01240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929273.1"
                     /translation="MSLVLPSSGQSSSMDHSGEIESSSSRERPVRWVGKYRILSELGR
                     GGMANVYLAVARGPGGVAKLVVLKALLPELASEPGALAMFLDEARLAAQLNHANVVQT
                     YEVGTEGERQVIVMEYLEGQSLAQIIKRAELSGRPMPPGMAVRIVINALEGLHYAHDL
                     SSYDGAPLSLVHRDVSPQNIFVTYDGQVKVLDFGIAKAASSSTHTATGIVKGKIAYMA
                     PEQMVGDNVDRRADVFSIGCMLWAFATGKKLWKDTPDVHIMRRVINAEIPNPQSVNPA
                     VDDELDRIVMRALAAERNDRYQTALELQEDLERYLDERSLTIKQKEIGNFVSTLFADT
                     RAELKALIERQITLAASDEFIGTADFSRSEVRTSTSGSGSGASAGSVARTLQSVDGTG
                     LSGSQSTAVGNPRARWVMVAGLLLLGGGVVYALGKSRQDPPPVVAAPRAPAPANPTVA
                     PPAQPTLATMQLRASPPEAQLFLNDEPLPSNPTAKVLPINGSVFRLRAEAPGYATGVE
                     EFSPSRDAEVEIALQKVGAPATEKHATRPGVRGPRQPVVATPAPKPSAAAPAPAPKPN
                     CSQPFFVDSDGIKKLRAECR"
     gene            complement(2480..>2980)
                     /locus_tag="VFQ35_01245"
     CDS             complement(2480..>2980)
                     /locus_tag="VFQ35_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929274.1"
                     /translation="LASPGFPELALAVTKVFRLLNAQRFLLCVYAANQRQARVALESS
                     GRDVVFRNESQFFPVGQIVPQHFLKPAKPSRLWLEPLRIGDEQLGYFIIDAEMPDAQS
                     HLELRHHLSTALSRIGLTRELRRLYAAERAGRVAGSSSTTSSTPPTPSASPPVPASVI
                     SKPPRP"
BASE COUNT          496 a          927 c         1044 g          513 t
ORIGIN      
        1 ggggccagcg cacgccgagc caccgaaggt gacgccgccg acgagggtcg acgcggcaga
       61 agctccgagc tcgacttcgc tcaaaacttc gagctcgtct ccgcctcccg ccacgttcgc
      121 ggcggagaac ccggttgacg aagccgtgtc gcggccgcgc tggccgtggg cggtggcggt
      181 actggtcatc gctgcgtcgc ttgcgatgct cgccgtgcgt tcgcatcgcg ctcccgcgcc
      241 cgtgaatccg acgaacgtcg gcgcgcccgc tcgctgagga gccccatccg agggctgctg
      301 ctcgttcagc ggcggctgcc ctgttcaggg aagaattggg gctcgttcag ggccgctgcg
      361 ctcttcaggg cccgccggcg ctcgttgagc ggggttcgcg gcggaagtcg cgcggcgcga
      421 tcgtgcggtg ccgacaaagc ggcgcgcccc ccaattgaga agcgcgcccg cggcgctcag
      481 cggcactcgg cgcggagctt cttgatgccg tcggagtcga cgaagaacgg ctgggagcag
      541 ttcggcttcg gcgcgggcgc cggagcggct gccgaaggct tgggcgctgg cgtcgccacg
      601 acgggctggc gggggccgcg gacgcccggg cgagtcgcgt gcttctccgt ggcgggggct
      661 ccgacctttt gcagcgcgat ctcgacctcg gcgtcgcggc tcggtgagaa ctcctcgact
      721 ccggtggcat aaccgggcgc ttcggctcgg agcctgaaga ccgaaccgtt gatgggaagc
      781 accttcgcgg tcggattcga aggcaatggc tcgtcgttta gaaagagctg cgcctctggc
      841 ggcgatgccc gaagctgcat ggtggcgagc gtgggctgag ccgggggtgc cacggttggg
      901 ttagccggag cgggagcgcg cggcgctgcc acgacgggcg gagggtcttg gcgcgacttg
      961 ccgagcgcgt acacgacgcc gccgcccaac aaaaggagcc cggcgaccat gacccaacga
     1021 gcgcgcgggt tgcccaccgc cgtcgattgg gagccgctca ggccagtccc atcgaccgac
     1081 tgcaaggtgc gcgcgaccga acccgccgaa gcacccgacc cagagccgga agtggacgtg
     1141 cgaacttcgg agcgcgagaa gtcggcggtg ccgatgaact cgtcgctcgc cgcgagcgtg
     1201 atctgtcgct cgatgagggc cttgagctcg gcgcgcgtgt ccgcgaacag cgtggagacg
     1261 aagttgccga tctccttctg cttgatcgtg agcgagcgtt cgtcgaggta acgctcgaga
     1321 tcttcttgga gctcgagcgc ggtctgatag cgatcgttgc gctcggcggc gagcgccctc
     1381 atcacgatgc gatcgagctc gtcgtcgacg gcggggttca cgctctgcgg attcggaatt
     1441 tccgcgttga tgacccgacg catgatgtga acgtcgggcg tgtctttcca cagcttcttg
     1501 ccggtcgcga aggcccacag catgcagccg atcgagaaaa cgtcggcacg acgatcgacg
     1561 ttgtcgccca ccatctgctc gggcgccatg tatgcgatct tgcctttgac gatgccggtg
     1621 gcggtatgcg tcgaggagct cgcggctttc gcgatgccga aatcgagcac ctttacctgg
     1681 ccgtcgtagg tgacgaagat gttctgcggc gatacgtcgc ggtgcacgag cgagaggggc
     1741 gcgccgtcgt acgacgagag gtcgtgcgcg tagtgcaacc cttcgagcgc gttgatgacg
     1801 atgcgaaccg ccatgccggg cggcattggt cgacccgaga gctccgcgcg cttgatgatc
     1861 tgcgcgagag actgcccctc gaggtactcc atcacgatga cttgtcgctc gccctccgta
     1921 ccaacttcgt acgtttgcac gacgttcgcg tggttcagct gagcggcgag gcgcgcctca
     1981 tccaagaaca tcgcgagcgc gccgggttcc gacgcgagct ccggcaagag tgccttcagc
     2041 acgacgagct ttgccacccc tcctggcccg cgcgcgacgg ccaagtagac gttggccatg
     2101 ccgccacgtc cgagctccga gaggatgcgg tactttccga cccagcgaac cgggcgctcg
     2161 cgagaggagc tagattcgat ttcgcccgaa tgatccatgc tcgagctctg accagatgaa
     2221 gggaggacca aggacattct gcgtccttgg atagtagcag tcccgtgcgc gaagcggacg
     2281 ggtgattggc cgttgcactg cagtgcagtg agtacgattc atagctcgtg tgaatcgaaa
     2341 ccacgagtgg gtaattcgac ggggccgtcc caccctgtaa gtatggggca attgcgcagc
     2401 aaaggctctc ttttcggtcg aattcgctcg ctgggactga agcgggtctc gaacaccgcg
     2461 aatcgcgctc gctttcggct cacgggcgcg ggggcttgct aatgacggac gctgggaccg
     2521 gtggcgacgc cgacggcgtc ggaggtgtgg atgaagtggt cgagctcgac ccggcgaccc
     2581 ggccggcgcg ttcagcagcg tacagtcgcc gcagctcgcg tgttagcccg atccggctca
     2641 gcgcagtgct caagtgatgg cgaagctcga ggtgtgactg cgcgtcgggc atctccgcgt
     2701 cgatgatgaa gtaaccgagc tgctcatcgc cgattcgcag cggctcgagc cagaggcgcg
     2761 agggcttcgc gggcttcaag aagtgctggg gcacgatttg gccgacgggg aagaactgcg
     2821 actcgttgcg aaaaacgacg tcgcgcccgc tcgattcgag cgctacgcgc gcttgccttt
     2881 ggttcgcggc ataaacgcaa agcaaaaatc gctgtgcgtt caagagccgg aaaaccttgg
     2941 tgaccgccag cgccagctcc ggaaagccgg gcgacgcgag
//
LOCUS       DASTKS010000059        11448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1791893, whole genome shotgun sequence.
ACCESSION   DASTKS010000059 DASTKS010000000
VERSION     DASTKS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791893"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFQ35_01250"
     CDS             complement(<1..1115)
                     /locus_tag="VFQ35_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929275.1"
                     /translation="MMSRRSKWVRHTWLMATCTTLGMSAWGYDARAQATTPPEEPPVA
                     PEPGAEKPANTAAPEPAAPPPTAAPSASPVDAAPPVAAPAPQGPPPSAIPDGEAPTKP
                     IPPAFPPAIPNIDFGARMQAGLKLQNPSDPTKMNDIGEILTADLLTSGQIHRYLKWQV
                     SVTLSYPGTPGSPSSVNVQPLDVIARIEPLPEFNVYIGRMLVVVDRFAPSGPWGMDEF
                     FFPGFFPLVAAPALPKAGAVGRDVGVTVWGAPLKGLVKYYLGAFNLYDPALNPLLSGR
                     LQVSLLSGEPAFYHRTTYFGTKDLLSLGVGGQYQKQGSVQNVPVTTPPTVPQTDDYSN
                     VTADLTFEKNIGDAGTISVVGAYYKYNGDFQRWKDFW"
     gene            1442..3325
                     /locus_tag="VFQ35_01255"
     CDS             1442..3325
                     /locus_tag="VFQ35_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HET9929276.1"
                     /translation="MDTEQHVVIQTGSHPEGPFAPPAVELLRRKGDQLLRSRYTLRAH
                     ARCSGEYLEEWARRSPERPFLLERDGIGWRGLNYGEMLEQVRRVGAWLLGRELSSERP
                     VAILSENSVEHAVLTLACLHVGVPVVPISPAYSLVSKDFGKLKAIVKTVRPGLVYASD
                     LRRYAAALGMLREVYDGPIVASEGSPSDAAVPFEALKSERDEAAVQRAFAAITPDTIA
                     KVMFTSGSTSEPKGVINTQRMLCSNQQAIAQIWPFLSRPPVLLDWLPWHHTFGGNHNF
                     NMVLRNGGTLYIDRGRPLPGEFDATLANLCEVSPTLSLNVPRAYDMLTAALRADSALC
                     RRFFSRLELLFYAGAALPQHVWDALANLSRETLGRDVPMVSSWGLTETAPTATVGHFQ
                     PDRAGIIGLPLPGSELKLVPNGDKTEVRVRGPNVTPGYLARPDLTASCFDEEGFFKTG
                     DAVRLVDPEHPERGLLFDGRIGEDFKLSTATWVNVGALRLEAITALAPVAQDVVITGH
                     DRDEVGLLVFPNLAGCRTLCGGLGGDADRLAVLEHPSVRARVAAGLGALTRAGTGSST
                     CARHALLLIEPASIDAGEITDKGYVNQRAVLTRRAALVERLYAAPPDPSVITASDAPG
                     ERS"
     gene            3372..4628
                     /locus_tag="VFQ35_01260"
     CDS             3372..4628
                     /locus_tag="VFQ35_01260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9929277.1"
                     /translation="MYESSERHALVARYDDIWLLGGLRTPFADYMSVLASVSPTDLGI
                     HVARALFQRLDVPAADVETIVAGNVAQSSFDAFYLPRHIGLYSGVPLGVPAVLVHRLC
                     GTGFEAIMSAADQIALGRAKLALCVAAESMSRNPVAAYTHRSGFKMGQVEFKDFLWEA
                     TLDTASNTRMGETAENLARVYGISREEVDGFAAQSFERAVSAWEEGYYQDEVAPVTSQ
                     QWALQGYQPRGIALPRKVERFDRDEHVRPSPLEVLQKLRPAFGKDGVQTGGNSSAIVD
                     GAAAALVASGDYVRAHGLKPLARIVAGGSVGVPPEVMGIGPAPLIRAMLRQLGLNQSS
                     VARFEINEAFGAQYLAVEKELALDRAKVNVHGGAIAIGHPLAASGVRLTIAAARAVRR
                     AKGEFALASACAGGGQGVGILLQHAV"
     gene            4657..5424
                     /locus_tag="VFQ35_01265"
     CDS             4657..5424
                     /locus_tag="VFQ35_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET9929278.1"
                     /translation="MQLNGSTFIVTGGSSGLGAATARHFHAGGARVVIADVNEDLGQR
                     LAEELGSRCAFCKTDVSVEADGQSAVDVARERFGALHGLVNCAGIGPAERVNGKKGPH
                     RLETFARVIQINLIGAFNMIRLAAAAMASNTPNAGGERGVIINTASVAVWDGQIGQAA
                     YAASKGGIVSMTLPIARELASIGVRVCTIAPGIFHTPMLAGLPAEVQDSLAKSVPFPP
                     RLGRPEEYAALAQQIVENEMLNGESIRLDGALRMAPR"
     gene            5433..7280
                     /locus_tag="VFQ35_01270"
     CDS             5433..7280
                     /locus_tag="VFQ35_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9929279.1"
                     /translation="MASLVTVTREENIAVVALNNPPVNALSHPVRVALLAALEELFAD
                     ATLGAIVIACEGRTFVAGADIREFGKAPLLPDLPDVIEFLDKAPQLTVAAIHGTALGG
                     GLELALACHFRVATPSAKLGLPEVTLGILPGAGGTQRLPRLIGVRPALELIVSGVPIS
                     AAQAQRLGIVDELGSDDVKASAVAFARRALAEGRRARRASELSATEGDAGELAEYERG
                     IAQRWRGFLAPFRCVEAVRAAVELPFAEGLERERALFRELMASAESAAQRHVFFGERE
                     VAKVPGLPEETPTRPVKSVVVVGAGALASGLAKCFTDARMAVTLLGDSAEELAAASSA
                     DLLLEAVAENADEKRATLARLGSVAKPGAILASTTSFLDLDALAGATGRPSDFVGMRF
                     ATPLEQMKLLEVVPCQRTATDAYATVMKLGRALGKIAVPVRAHVANRLLTRRNREIGL
                     LLEEGASRGDIDAALVDFGFPTGPFAELGSSTLAGSHAEKGAAAVTDPSVAALLESPR
                     NGRGGRGRSVSAEEIRDRCLYAVVNEAARVLDEGLAARALDIDMIWVHGHAFPVYRGG
                     PLFFADQVGLSVVHQHIQRYRAEVGEEYWSPAPRIERLAKEQGRFYGKT"
     gene            7297..9378
                     /locus_tag="VFQ35_01275"
     CDS             7297..9378
                     /locus_tag="VFQ35_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9929280.1"
                     /translation="MASSRTNLERLFAPASVAVVGASASPEKAGYQAMLALSAFGGDV
                     FAINPKGGSVLGRTALPSLRALSRPVDLVVFAVPAPACVEAVREAIECGCGGGLIVSG
                     GFSESGAAGAALEAELERSCRQSGFRLLGPNTAGFVNKQLSLTASFLPSAERIPSGHV
                     AVVAQSAGINLTVSFLLAKLGYGVSCAVGLGNSVDVDAADVLEFVAGQPETKSIALHL
                     EGVRDGRRLYEAILRISPVKPITVFTVGKTDIGEFARSHTGNLIGSYALRSSALEQAG
                     AVLVESTEELAIAAAALSLHRLSPKPKPGVGVITAQAGAGLAMLDLLKSKGVAVPMLT
                     APTRARIGRHLSPMTYQENPVDTGRPGPSFAEVVTAVAQDDDIDAVITYALSEPAALL
                     PSECLPAIKRGVTTPILFGTMGPRDEVSETSVALRAQGFYVAESPEELARAGTALARD
                     AAQRARVARRKGHVRFPVATLPLSPPDEHTAKQTLDALGIPTPRRVVCATREDAREAL
                     RQLEKPVVAKILSPEIEHKTELGGVQLNVRDEAALDAAIARLDAIPLTSRRRYLIEEM
                     APAGLELIVSATRDVSFGPTVMVGLGGTFAEALRDTVTRLAPLGLEEAEEMLEQLRAA
                     PLLAGWRGGPKLDRTAVARCIVALGDFLNANGSVRELEINPLRVYPSGVLALDALLIL
                     ERSAALKANTP"
     gene            complement(9497..10468)
                     /locus_tag="VFQ35_01280"
     CDS             complement(9497..10468)
                     /locus_tag="VFQ35_01280"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9929281.1"
                     /translation="MPASPKKKNLRPPAQRRSGSVAKRGTTKRSEPEQVDSNAGPSDL
                     HQVLAQRVRDARARRFMTRKALAEESGISLAYLARVESGTGNISLGLLQRLALALNLP
                     IESFLASEQAPNADFTMIVEFLKRQSAERLALIRRQLFDSHDRAHRVALVGIRGVGKS
                     TLGPALAERLAVPFVELNREIEKEAGLGVSEIFTVYGQHGYRVIERRCLERIIVNHPK
                     VVLATAGGIVAETATYEVLLSSFFTIWLKASPEVMFERVLAQHDARIASPELRHEALE
                     NIERTLEARRHLYELAPAAFDTSGKSIEQIVRGLLTLLPGGVRPQAS"
BASE COUNT         1857 a         3669 c         3853 g         2069 t
ORIGIN      
        1 aaccagaagt ccttccagcg ctgaaaatcc ccgttgtact tgtagtaagc gccgaccacc
       61 gagatcgtgc ccgcgtcccc gatgttcttt tcgaaggtga ggtccgcggt gacgttgctg
      121 tagtcgtcgg tttgcggcac ggtggggggc gtcgtcaccg ggacgttctg taccgagcct
      181 tgcttctggt actgaccgcc gacaccgagc gagagcaggt ccttcgtccc gaagtacgtc
      241 gtacggtggt agaaggcagg ctcaccgctc agcaagctga cttggagccg cccactgagc
      301 agcgggttca acgcggggtc gtaaaggttg aacgcgccca ggtagtattt gaccagtccc
      361 ttcagcggcg cgccccacac ggtcacgccg acatcgcgcc ccaccgcgcc tgccttcggt
      421 agggctggcg ctgcaaccag cggaaagaag ccggggaaaa agaactcatc catgccccag
      481 gggccgctcg gcgcgaaccg gtcgacgacg accagcatgc ggccgatgta aacgttgaac
      541 tcgggcagag gctcgatgcg cgcgatgacg tcgagcggtt gaacgttgac cgagctcggc
      601 gaccctggag ttccaggata ggagagcgtt acgctcactt gccacttcag gtagcggtga
      661 atctgacccg aggtgaggag atcggcggtc aggatctcac cgatgtcgtt catcttggtg
      721 ggatcgctcg ggttctgcag cttgagcccg gcttgcatcc gtgctccgaa atcgatgttc
      781 ggaatcgcgg gtgggaaggc cggcggaatc ggtttcgtgg gcgcctcacc gtcggggatt
      841 gccgaaggtg gcggaccttg aggagcgggc gcggcgacgg gcggagcggc atcgaccggc
      901 gatgcgctcg gggctgccgt cggaggcggg gccgccggtt ctggggcagc ggtgttcgcg
      961 ggcttttcgg cgccgggctc tggcgccacg ggcggctctt ccggaggcgt cgtcgcctga
     1021 gctcgcgcgt cgtaccccca cgccgacatg ccgagcgtcg tgcacgtcgc catcaaccac
     1081 gtatgccgaa cccatttcga gcgtcgactc atcatccatt ctcccggccg caactccgtg
     1141 tccccacccg cctcgtttga tgctggttcg ccggtgctca agtgagagcc agcacagccg
     1201 tcgtcctcgg cgagcagccg agatgtacta tagtatacgt ctgctgggta ctctataata
     1261 atctttgtgg cgaaatcggc ggctccgccg tagcagaccg cgtgaaatgc ggacaaatgt
     1321 gaaaagttcc aatggggccg gttactgcgt cgcgctggac aggtagcatc tactatagta
     1381 catgttggtc gacggttgga ctgcgccgag gtgcgctccc accccgtgcg gtcatgagtt
     1441 gatggatacc gagcagcatg tcgtgatcca aactggctcc catcccgagg ggccgtttgc
     1501 gcctcccgcg gtcgagctcc ttcgccgcaa gggagatcaa ctgcttcgct cgcgctacac
     1561 gctccgggcc cacgcgcgat gtagtggtga gtatctggag gagtgggcgc gccgctcgcc
     1621 ggagcgcccg ttcctcttag agcgagatgg gattggctgg cgcggtctca attatgggga
     1681 gatgctcgag caggtgcgcc gcgtgggagc gtggctcctc gggcgggagc tttcttccga
     1741 gcgccccgtc gcgatcttgt ccgagaacag cgtggaacac gccgttttga cgctcgcgtg
     1801 tctgcacgtc ggcgttcccg tcgtcccgat ttcgcctgcg tactcgctgg tttccaagga
     1861 cttcggcaag ctcaaggcaa tcgtgaagac ggtgcgcccg gggctggttt acgcgagtga
     1921 cctccggcgg tacgcggccg cgctcggcat gctgcgagaa gtgtatgacg gtcccatcgt
     1981 cgcgagcgag ggctcgccga gcgacgcggc cgtgccgttc gaggcgctca agagcgagcg
     2041 cgacgaggcc gcggtgcagc gcgcgttcgc cgcgatcacg ccggatacca tcgcgaaggt
     2101 gatgttcacc tcggggtcga cgagcgagcc caagggtgtg atcaacaccc agcgcatgct
     2161 gtgctcgaat cagcaggcga tcgcgcagat ttggccgttt ttgagccgtc cgcccgtgct
     2221 gctcgactgg ctgccgtggc accacacttt tggcgggaac cacaatttca acatggtcct
     2281 gcgaaacggc gggacgctgt acatcgatcg aggtcgacca ctgccgggtg aattcgacgc
     2341 gacgctcgcc aacttgtgcg aggtctcgcc gacgctctcg ctcaacgtgc ctcgcgcgta
     2401 cgacatgttg accgccgcgc tgcgtgcgga ttcagcgctc tgccgccgct tcttcagtcg
     2461 cctcgagctc ctgttctacg ctggagcggc gctgccgcag cacgtgtggg acgctctcgc
     2521 gaatctctcg cgggagacgc tcgggcgtga cgtgccgatg gtctcttcgt ggggtctgac
     2581 cgagaccgcg ccgacagcga cggtcggcca ttttcagccc gaccgcgccg ggatcattgg
     2641 actgccctta ccggggagcg agctcaagct cgtgcccaac ggggacaaga ccgaggtgcg
     2701 cgtgcggggc cccaacgtta cgcccggcta cctcgctcgt cctgatttga cggcgagctg
     2761 cttcgacgaa gagggcttct tcaaaacggg ggacgcggtg cgactcgttg accccgagca
     2821 ccccgagcga ggcttgttgt tcgacggtcg catcggcgaa gacttcaagc tcagcacggc
     2881 tacgtgggtc aacgtgggcg cgctgcgcct ggaggccatc accgcactcg cgccagtcgc
     2941 gcaagacgtc gtcatcactg ggcacgatcg cgacgaagtc gggctcttgg tgttcccgaa
     3001 cctggctggc tgtcgcacgc tgtgcggtgg gctcggaggc gacgctgacc ggctcgcggt
     3061 gctggagcac ccctctgtgc gtgcgcgggt cgcggcgggt ctcggcgcgt tgactcgtgc
     3121 cggcaccgga tcgtcgacgt gtgctcgcca cgcgctcctg ctaatcgagc cggcatcgat
     3181 cgacgccgga gagataaccg acaagggtta cgtcaaccag cgagccgtct tgacgcgccg
     3241 cgccgcgctc gtcgagcggc tctacgccgc gccgcccgat ccgagcgtca tcaccgcgtc
     3301 cgacgcgccg ggagagcgca gctgacgatc ggcgggagcg ctttcccgta agcacgacga
     3361 tggagtctca catgtacgaa tcttcggaac gtcacgcgct cgtcgctcgc tacgacgaca
     3421 tttggctgct cggtgggcta cgcacgccgt tcgccgacta catgagcgtg ctcgcgagcg
     3481 tttctccgac ggatctcggc attcacgtcg cgcgagctct ctttcaaagg ctggacgttc
     3541 ctgcggccga tgtagagacg atcgtcgcgg ggaatgtcgc tcagtcgagc ttcgacgcgt
     3601 tctacttgcc gcgtcacatc ggcctctact ccggggtccc gctcggagtt ccggccgtgc
     3661 tcgtacatcg attgtgtggg accggcttcg aagccatcat gagcgcggcc gaccaaatcg
     3721 cgctcggcag agccaagctt gcgctctgtg tggccgccga atccatgtcg cgcaacccgg
     3781 tggctgccta cacgcaccgc agcggcttca agatgggcca ggtcgagttc aaggacttcc
     3841 tgtgggaggc gacgctcgac acggcttcga acacgcgaat gggcgagacg gccgagaacc
     3901 tcgcgcgcgt ttacggcata tcgcgcgagg aagtggacgg gttcgccgcc caaagcttcg
     3961 agcgcgcggt gagcgcgtgg gaggaaggct actatcaaga cgaggttgcg cccgtgacct
     4021 cgcagcagtg ggcgctgcag gggtaccagc cgcgcggcat cgcgctgcct cgaaaggtag
     4081 agcgcttcga tcgagacgag cacgttcggc ctagtccctt ggaggtgctg cagaagctgc
     4141 gcccggcgtt cggcaaagac ggtgtgcaga ccggcggcaa cagctccgcc atcgtcgacg
     4201 gcgctgcggc ggcgctcgtc gcctcgggcg actacgtccg cgcccatggc ttgaagccgc
     4261 tcgcacgtat cgtcgcgggc gggtcggttg gcgtaccgcc ggaagtgatg ggaatcggcc
     4321 ccgcgccgct gattcgcgcg atgctccgac agctcgggct aaaccagagc agcgtcgctc
     4381 gcttcgaaat caacgaggcg ttcggggctc aatacctcgc cgtggagaaa gagctcgcgc
     4441 tcgaccgcgc taaggtcaac gtccacggcg gcgccatcgc gattggccac ccactcgcgg
     4501 cctccggcgt acgtctaacc atcgccgccg cacgcgccgt gcgccgagca aagggcgagt
     4561 ttgcgctcgc ctctgcctgc gcaggcggag gccaaggcgt gggcattctg ctgcaacacg
     4621 ccgtgtgatc aacgttcaac tcatcgagaa caagacatgc aactcaacgg atcgaccttc
     4681 atcgtcacgg gcggcagttc aggcctcggc gccgcgacgg cgcggcactt tcacgccggc
     4741 ggagcccgcg tggtcatcgc cgacgtcaac gaggacctcg gtcagcgcct cgccgaagag
     4801 ctcggctcgc ggtgtgcttt ctgcaagacc gacgtcagcg tcgaagccga tgggcagtcg
     4861 gcggtggacg tcgcgcgtga acgttttggt gctttgcacg ggctcgtcaa ttgtgcgggg
     4921 atcggccccg ccgagcgcgt caacgggaag aagggccctc accggctcga aacgttcgcc
     4981 cgcgtgattc aaatcaactt gatcggcgcg ttcaacatga ttcgcctcgc tgccgctgcg
     5041 atggcgagca acactcccaa cgccggcgga gaacgcggcg tcatcatcaa caccgcgtcg
     5101 gtcgcggtgt gggacggtca gattgggcag gcggcgtacg cggcttccaa gggcggcatc
     5161 gtctcgatga ctctaccgat agcgcgtgag ctcgcgagca tcggggttcg cgtgtgcacg
     5221 atcgctcccg gcatcttcca cactccgatg ctggccgggc tcccggcgga ggttcaggat
     5281 tcgctcgcca agagcgtgcc gttcccgccg cggctcggcc gccccgagga gtacgcggcg
     5341 ctcgcccagc aaatcgtcga gaacgaaatg ctgaacggcg aatcgattcg tttggacggc
     5401 gcacttcgca tggcgccgcg ctgagctcag ccatggcaag cctagtcacc gttacccgcg
     5461 aggagaacat cgcggtcgtt gccctgaaca atccgcccgt caacgcgctc tctcatccgg
     5521 tgcgggttgc gcttctcgcg gcgctggagg agctcttcgc tgacgccact ctcggcgcca
     5581 tcgtcatcgc gtgcgagggg cgtaccttcg tcgcgggcgc ggatattcgt gagttcggca
     5641 aagcgcccct attgccggac ttgccggacg tcatcgagtt cctcgacaaa gcaccgcagc
     5701 tgacggtcgc ggctatccat ggaaccgcgc tcggcggtgg gctcgagctc gcgttggcct
     5761 gtcactttcg cgtagcgact ccgagcgcga agctcggctt gcccgaagtg acgctcggca
     5821 ttttgcccgg cgcgggtggc acacagcgcc tcccacgcct catcggcgtg cgtcccgcgc
     5881 tcgagctcat cgtatcgggg gttcccattt cggcagccca ggcgcaacgc ttgggcatcg
     5941 tcgatgagct cggtagcgac gacgtgaagg cctctgcggt cgctttcgca cggcgtgcgc
     6001 ttgccgaagg caggcgcgcg cgccgcgcga gcgagctcag cgcgaccgag ggagacgcgg
     6061 gcgagttggc cgagtacgaa cgaggcatcg cgcagcgctg gcgcggcttt ctggcgcctt
     6121 ttcgctgcgt ggaggccgtg cgcgcggccg tcgaactgcc ttttgccgaa ggcctcgagc
     6181 gcgaacgggc gctgtttcgc gagctgatgg cgtcggcgga gtcagctgcg cagcgtcacg
     6241 tgttcttcgg tgagcgagag gttgcaaagg ttcctggcct acccgaagaa acccccaccc
     6301 gacccgtgaa gtccgttgtc gtggtcggag ctggcgcgct cgcgagcggg ctcgcgaagt
     6361 gcttcaccga tgctcgcatg gcggttacgt tgctgggcga cagcgcggaa gagctggccg
     6421 ccgcgagcag cgcggatctg ttgctggaag ccgtcgccga gaacgcagac gaaaagcggg
     6481 cgacgttggc tcggctgggc agcgtcgcga agccgggcgc cattctcgcg agcacgacgt
     6541 cgtttttgga tctcgacgcg ctcgcgggcg ccacgggtcg accgagcgac ttcgtcggca
     6601 tgcgcttcgc gaccccgctc gagcagatga aattgttgga ggtcgtgccg tgtcagcgca
     6661 ccgcgaccga cgcttatgca accgtgatga agttgggtcg cgccctcgga aagatcgcag
     6721 tgccggtacg agcgcacgtt gcgaaccggc tgctcacgcg ccgcaatcgt gaaattggcc
     6781 ttctgctgga ggaaggcgca tcgcgtggtg acatcgacgc ggcgctcgtc gacttcgggt
     6841 tcccgacagg accgttcgct gagctcggct catccacgct cgcgggctcg cacgcggaaa
     6901 agggcgccgc ggcggtcact gacccgagcg tagcggccct gctcgaaagc cctcggaacg
     6961 gccgcggtgg gcgcgggcga agcgtctccg ccgaagaaat tcgggaccgt tgtctttacg
     7021 cggtcgtgaa cgaggcggct cgcgtactcg atgagggact cgcagcgcgc gcgttggaca
     7081 tcgacatgat ttgggtccac ggccatgcgt tccccgtata ccggggaggg ccgctcttct
     7141 tcgccgacca ggtcgggctc tccgtcgttc accaacacat ccagcgatac cgggcggagg
     7201 tgggcgagga atactggtca cccgcgccgc gcatcgaacg tttggccaag gagcaaggcc
     7261 gcttctacgg aaagacctga gaggtcgagc atcgcgatgg catcgtctcg cacgaacttg
     7321 gagcgcctgt ttgcgccggc gtcggtcgcc gtggtcggtg cgtccgcctc gcccgagaag
     7381 gccggctacc aggcaatgtt ggcgctatcg gcctttggtg gtgacgtgtt cgcgatcaac
     7441 ccgaagggcg gttcggtcct cggacgcacg gcgcttccct cgctgcgcgc gctgtcccgc
     7501 cccgtcgatt tggttgtctt cgccgtcccc gcgcccgcgt gcgtcgaagc cgtgcgcgag
     7561 gcgatcgagt gcggttgcgg cggcgggctc atcgtcagcg gaggattttc ggagtctggc
     7621 gcggccggcg cggcgttgga ggcggaactc gagcgctcgt gccggcagtc ggggttccgt
     7681 ctgctcggcc cgaacaccgc cggattcgtc aacaagcagc tgtcgctcac cgcaagcttc
     7741 ctcccttcgg cagagcggat tccctccggt cacgtcgccg tcgtcgccca aagcgcgggc
     7801 atcaatctca ccgtcagctt cttgctcgcc aagttgggct acggcgtttc gtgcgcagtt
     7861 gggctcggta actcggtgga tgtcgacgcg gcggacgtgc tcgagttcgt cgcgggtcaa
     7921 cccgagacca agtccatcgc gctgcacctc gagggtgtgc gagacggtcg tcgtttgtac
     7981 gaggcgatac tgcgcatatc gccggtcaag ccgattacgg tgttcacggt cggaaaaacg
     8041 gatatcggag aattcgcgcg ctcgcatacc ggcaacttga ttggttccta tgcgctccgc
     8101 tccagcgcgc tcgagcaggc gggcgcggtg ttggtcgagt cgaccgagga gctcgcgatc
     8161 gccgcggccg ccctgtcttt gcaccgactg tcacccaagc cgaagcccgg cgtcggcgta
     8221 atcaccgcgc aagcgggagc agggcttgcc atgttggatc tgctgaagtc gaagggggtg
     8281 gcagtgccga tgctcaccgc gccgacgcga gcgcggatcg gccgccacct gtctccgatg
     8341 acgtaccagg agaaccccgt cgataccggg cgcccaggtc catcgttcgc cgaagtcgta
     8401 accgctgtcg cgcaggacga cgacatcgac gccgtaatca cctacgcgct gagcgagccc
     8461 gcggcgttac tgccctccga gtgcttacct gccataaagc gcggcgtgac gacgccgatc
     8521 ctgttcggga ccatgggacc gcgcgacgaa gtgagcgaga cgagcgtggc gctccgagcg
     8581 cagggattct acgtcgccga gtcgcccgaa gagttggcgc gcgcggggac cgctctagcg
     8641 cgcgatgcgg cgcagcgcgc gcgtgtcgcg cggcgcaaag gtcacgtgcg ctttcccgtc
     8701 gcaaccttgc cgctgtcgcc gcccgacgag cacaccgcga agcaaaccct cgacgccctc
     8761 gggatcccga cgccgcgacg ggtggtttgc gcaacgcgcg aagacgcccg cgaggcccta
     8821 cgtcagctcg agaagccagt cgtggccaag atcctctcgc ccgagatcga gcacaagacc
     8881 gagctcgggg gcgtgcagct gaacgtccgc gacgaggccg cgctggacgc cgcgattgcc
     8941 cggctcgacg cgatccctct gacgtcgcgg cgccgctacc tcatcgaaga gatggcgcct
     9001 gctggcctcg agctcatcgt ctccgcgacg cgagacgtga gcttcggtcc gaccgtaatg
     9061 gtagggctcg gcggcacgtt cgcggaggct ttgcgcgaca cggtgacgcg gcttgctccg
     9121 ctcggtctcg aagaggccga ggagatgctc gaacaattgc gagccgcgcc gctgttggcg
     9181 ggctggcgag gcggacccaa actcgaccgg acggcggtcg cacgatgcat cgtcgcgctc
     9241 ggcgacttct tgaatgcgaa cgggagcgtc cgtgagctcg aaatcaatcc gctacgtgtc
     9301 tatccgagcg gcgtcctcgc gctcgacgcg ttgttgatcc tcgagcgcag tgccgccctc
     9361 aaggcgaata cgccctagtc tagcagggcg ttaaaaagcg ccctgcgtca cgacagattc
     9421 cgcgcagagt gcccgcgtgg ctggagtctc ttccgaattc attcggccat gacgagtcga
     9481 gcaccgggcg cgagcctcag gaagcctgag gacgcacgcc tccgggcagc aaagtcaaca
     9541 ggccgcgcac gatctgctcg atgctctttc cgctggtgtc gaaggcggcc ggcgcaagct
     9601 cgtacaggtg ccgccgcgcc tccaacgtcc gttcgatgtt ttcgagcgcc tcgtggcgaa
     9661 gctcggggct cgcgatgcgc gcgtcgtgtt gggcgagcac gcgctcgaac atcacctcgg
     9721 ggctggcctt gagccagatc gtgaagaacg aactcagcag gacctcgtag gttgccgttt
     9781 ctgccacgat gccaccggcc gtcgcgagga cgaccttggg gtgattgacg atgatgcgct
     9841 cgaggcagcg acgctcgatg acgcggtacc catgctgccc atataccgtg aagatttcgc
     9901 tcacgccgag gcccgcctcc ttctcgatct cgcgattgag ctcgacgaaa gggaccgcga
     9961 ggcgttcggc gagcgcgggt cccaaggtcg actttccgac tccgcgaatg ccgaccagag
    10021 cgacgcggtg cgcgcgatca tgactgtcga acagctgtcg ccgaatcaac gcgaggcgct
    10081 ccgcgctctg ccgcttcagg aactcgacga tcatcgtgaa gtcggcgttg ggagcttgct
    10141 ccgaagcgag gaagctctcg atcggtaagt tcagcgcgag tgccaaccgt tgcaggaggc
    10201 ccagcgaaat attgccggtt ccactctcga cccgagcgag gtacgcgagc gaaatccccg
    10261 actcttccgc gagcgctttg cgtgtcatga accgacgcgc tcgggcatcg cgaacgcgtt
    10321 gagccaagac ctgatggaga tcactcgggc cagcgttcga gtccacctgc tcgggctcgc
    10381 ttcgcttcgt cgtgccgcgc ttcgcgacgg acccgctgcg ccgctgggct gggggtcgga
    10441 ggttcttctt cttgggcgag gccggcatcg gcgtgcatcg taatggctga agcctgcgct
    10501 gtcctgtccc cttcgagcag atcgtgcggc tgtctgccgc ctggacgccg cgctgtacgt
    10561 gcagttttct cggttggtcg tcgaaggaga gaggggaggg cacacggccc tgggttcagt
    10621 cgtctttcag ggtgcgctcg ctccatgctt ggcgctgccg gacctccgca atcggcatgg
    10681 gcgtcggccg ccgccgctga gattgctgga agtcgtcttc ggggccaggc gtgcgggggt
    10741 cgagctcggt cgcggcctgc gcgccccacg cgcaccacga ctttgcagcg ccgcagagtc
    10801 cgcacggcgt cgcgttcgac gcaaacgcgg tgctgcgatc tcgatctcga tcgctgtcga
    10861 gcgactcgtc tttgacattg ctcgcattcg gcgagcgcgt tcctgcgcga gcccacccgt
    10921 gactgcgctt cgaccgttcg ttcgcgagca gtcgttgggc aggacgaact cttgctccga
    10981 gacgagcggg tcaccgcgag gttcgaagtt cgtcggtgtc gatcacgacg agattcggtc
    11041 agcgccgaac cgtcgttgag aacgagcgtt cgaaaacgag gtttccttaa ttgctgttcg
    11101 agatacagca tagaaagccg cgtatatcgc attcgcgatc atcgcgcgtc atttcgaatt
    11161 ggtgaatcga ggtcgtcgag agagtggcgc tcctcgtcgc ttcgtcgcga ttccacgacg
    11221 gaacgagccg ggtaacgcga ctgctagaaa gtctggcaga ggctcttcgt gcccaggacg
    11281 gcgcgagccc gccacgacca ggagcaccaa tgaagcccgt ttgcgtgttg gttgttgtgg
    11341 cactcgtcaa tgccgcttgc tcgactcaag acggttcgag cggttcgggc gggagcactg
    11401 gttcgggcgg agctcactcc acggcatcgg gcggttcgac gcagtcca
//
LOCUS       DASTKS010000060         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_899926, whole genome shotgun sequence.
ACCESSION   DASTKS010000060 DASTKS010000000
VERSION     DASTKS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899926"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1216
                     /locus_tag="VFQ35_01285"
     CDS             251..1216
                     /locus_tag="VFQ35_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET9929282.1"
                     /translation="MGTVRDFIQHHYRHFNAAALVDAARAWENHLAKGGKMLLTMGGA
                     MSTAEMGIVIAELIRKEKIHGISLTGANLEEDVFNLVAHESYRRVPNFRELRPEDEKA
                     LLDQGLNRVTDTCIPEHEAMRKIEKKMLALWSSAQRKGERYFPHEYFFQLLRTKELEP
                     EYEIDPKNSWLLAAAEKNLPLFVPGWEDSTLGNMFVAGVIRGEYGYQIMKGGTEYMGR
                     LIEWYKELDRQSSIGFFQIGGGISGDFPICVVPLMDQDLGWDVRTWGYFCQISDATTS
                     YGSYSGAPPGEKITWGKLAVDTPRFIIESDATIVFPLVAAYLLDM"
     gene            1304..2380
                     /locus_tag="VFQ35_01290"
     CDS             1304..2380
                     /locus_tag="VFQ35_01290"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET9929283.1"
                     /translation="MQTPSRSRFVGCLLGGALGDALGYPIEFEGPGQVLLDRYGSTPP
                     STLLYQGGEVALVSDDTQMTLFTAEGLIRARRAGEADPTRYVLSAYQRWYATQAMRPG
                     QAPLVRTPQTQGLLLADARLHARRAPGNTCLSALAASFAGRPQPSVENPPNYSKGCGA
                     VMRSAPFGLVSESAEAAFLRARDAGVLTHGHPSGYLSGAYFAALVFELARGTSPSLAL
                     ERCDALLAREAEHDELRAILRRARELLPDGLPSTEDIERLGEGWTGEEALAIALVCAL
                     TTGESEHEVREALWRSVAHGGDSDSTGSLTGNLLGAMYGAEALPKAWLSELEMTDLIE
                     RVAVDLWHADEAPAKLDSSAYPLG"
     gene            complement(2405..3511)
                     /locus_tag="VFQ35_01295"
     CDS             complement(2405..3511)
                     /locus_tag="VFQ35_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9929284.1"
                     /translation="MEVPDVLDTVVIGGGVIGLSVARALALAGREVFVLEAEAKLGMH
                     TSSRNSEVVHAGIYYEPGSLKATLCRAGRQLLYAYCERYGVPFARPGKLILATRDEEV
                     QRLEEIYSRAQANGVDDLAWLTSTDVSAREPNIVAVRALFSPSTGIVDSHALMAAFKR
                     DAVANGAHVITSAAVLRGHATDSGFVLELGGRNAGTVRCRALVNAAGLRAPSVSRSLT
                     GIPNSAIPGEHYAKGHYFVLDGAAPFRHLIYPVPVSGGLGIHVTLDLAGRVRFGPDVT
                     WIEGIDYSFDTRRVTSFYDAIRPYYPALADGSLSPGYTGIRPKVARGDTPHDFVIRGP
                     RDSGVSGFCALYGIESPGLTAALAIADHVRDELT"
BASE COUNT          688 a         1178 c         1190 g          584 t
ORIGIN      
        1 cccgctcaaa gaagcgggag gcgtcgagaa gcgatcaacc atcggaattc cgctgcgccc
       61 gaagaacccg aaaaaacgcc gccgagccta gcacggaatc gtcacagaaa accccgagcg
      121 aacgattgct ggcccttcgc cgaaaaaccg gcgtccgttt cgagaaatgg cttcgcgcgt
      181 gggcttcgct cgcttgactt tgctggggaa acgcggaaaa gcgtcccccg gaaccgcgag
      241 gagcatcacc atgggtacgg tccgcgactt cattcagcat cattaccgcc acttcaacgc
      301 cgccgcgctc gtggacgccg cgcgcgcctg ggaaaaccat ctggccaaag gcggcaagat
      361 gctgctcacc atgggcggcg cgatgagcac cgccgagatg ggcatcgtca tcgccgagct
      421 catccgcaaa gagaagattc acgggataag tctcactggc gcaaacctcg aagaggacgt
      481 gttcaacctg gtggcgcacg agagctaccg gcgggtaccg aatttccggg agctccgccc
      541 cgaggacgag aaggcgttgc tcgatcaggg cctgaaccgc gtgaccgaca cctgcatccc
      601 cgagcacgag gcgatgcgga aaatcgagaa aaaaatgctc gcgttgtggt cgagcgctca
      661 gcgtaaaggc gaacgctact ttccgcatga gtactttttt cagctcctgc gcacgaagga
      721 gctcgagccc gaatacgaaa tcgacccgaa gaactcgtgg ctccttgccg cggcggaaaa
      781 gaacctcccc ctgttcgttc caggctggga agactcgacc ctcggcaaca tgttcgtggc
      841 tggcgtgatc cgcggcgagt acggctacca gatcatgaag ggcggaaccg agtacatggg
      901 ccgcctcatc gagtggtaca aggagctcga ccgccagtcc tcgatcggct tcttccagat
      961 cggcggcggc atctccggcg acttcccgat ctgcgtcgtc ccgctcatgg atcaggatct
     1021 cggttgggac gtgcgcacct ggggctactt ctgccagatc tccgacgcaa cgacgtcgta
     1081 cggctcctac agcggtgcgc ctcccggaga gaagatcacc tggggcaagc tcgcggtgga
     1141 cacgccgcgc ttcatcatcg agtcggacgc aaccatcgtc ttcccgctgg tcgccgccta
     1201 cttgctcgac atgtgatagc ggtcacgaac gccggcgcgc gatggcaagg gtcgcgcgcc
     1261 gctcgtgagc cagccaggcc ggtgcgctag ggtgtaggcg ccgatgcaaa cgccatctcg
     1321 gtctcgcttc gtaggctgcc tcctgggagg cgctttgggc gacgcgctcg gttatccaat
     1381 cgagttcgag ggcccagggc aggtgttgct cgaccgctat gggtcgacgc cgccgtcgac
     1441 cttgctctac caaggtgggg aagtcgctct ggtgtccgat gacacacaga tgacgctatt
     1501 caccgcggag gggctcatcc gtgcgcggcg cgcgggggaa gccgatccga ctcgctacgt
     1561 gctcagcgca taccagcgct ggtatgcgac gcaagcgatg cggcctggac aggctccgtt
     1621 ggtgcgaacg ccgcaaaccc aggggttgct gctggcagat gcgcgcctgc acgcccgacg
     1681 cgcgccgggg aacacctgcc tttcagcgct tgccgcaagc ttcgcgggtc gcccccagcc
     1741 cagcgtggag aatccgccga actacagcaa gggttgcggc gccgtgatgc gctcggctcc
     1801 gttcggcttg gtgagcgaga gcgccgaagc agcgtttttg cgagctcgcg acgcgggcgt
     1861 cctcacccac ggtcatccga gcgggtacct ctcgggcgcg tacttcgcag ctttggtgtt
     1921 cgagctggct cgcggcacgt ctccgtccct cgccctcgag cgctgcgacg cgctgctcgc
     1981 gcgcgaagcc gagcacgacg agctgcgcgc gatcttgcgg cgcgcgcggg agctgctgcc
     2041 cgacgggctg ccgagcacgg aggacatcga gcggctcggc gaaggctgga ccggcgaaga
     2101 ggccttggcc atcgcgctcg tctgcgcgct caccaccggc gagagcgagc acgaagtgcg
     2161 cgaggcgctc tggcgttccg tcgcgcacgg cggtgacagc gacagcaccg ggagcctcac
     2221 cggaaacctg ctcggagcca tgtacggcgc cgaagccctg ccgaaggcct ggctcagtga
     2281 gctcgagatg accgacctga tcgagcgagt cgccgtcgat ctctggcacg ccgacgaggc
     2341 tcccgccaag ctcgactcga gcgcgtaccc actcgggtga cgcagagcgc gccgcggcga
     2401 cttttcatgt cagctcgtcg cgaacgtggt cggcgatggc gagcgcggcc gtaagtcccg
     2461 gtgattcgat gccgtacaac gcgcagaaac cggaaacgcc cgaatctcga gggccgcgaa
     2521 tgacgaagtc gtgcggagtg tcaccgcgcg cgacctttgg acggattccc gtgtaccccg
     2581 gcgagaggct gccgtccgcg agcgccgggt aataggggcg aatcgcgtcg tagaaggacg
     2641 tgacccgccg cgtgtcgaag gagtagtcga tgccctcgat ccaagtaaca tcggggccga
     2701 agcgcacccg ccctgccaaa tcgagcgtca cgtggatgcc caaaccacca ctcaccggaa
     2761 ccggatagat gagatggcga aacggtgcgg cgccgtccaa cacgaagtag tggcccttgg
     2821 cgtagtgctc gcccgggatg gcggaattgg gaatgccagt gagcgaacga gagacgctcg
     2881 gcgcacgcaa ccccgccgcg ttcacgagag cgcgacagcg aaccgttccc gcgttgcgcc
     2941 cgcccaattc gagtacgaat cccgagtccg tcgcatggcc gcgcaggacg gccgcggacg
     3001 tgatgacgtg cgcaccgttc gcgaccgcgt cgcgcttgaa tgcagccatg agagcgtggc
     3061 tatcgacgat gccggtcgac ggcgaaaaca aggcacgcac ggcgacgatg tttggctcgc
     3121 gagcgctaac gtcggttgac gttagccatg cgagatcatc gactccgttc gcctgagcgc
     3181 gcgagtagat ttcctcgaga cgctggacct cctcgtcgcg ggtggcgagg atgagcttcc
     3241 cgggtcgcgc gaagggaacg ccgtagcgct cgcaataggc gtagagaagc tgacgacccg
     3301 cgcggcacaa ggtggccttc agagagcctg gttcataata gatgccggcg tgcacgacct
     3361 ccgagttgcg agaactcgtg tgcatgccga gcttcgcctc tgcctcgagc acgaagactt
     3421 cgcgaccggc gagagcgagc gcccttgcga cagaaagccc gatgacgccc ccgccgatca
     3481 ccaccgtgtc gagaacgtcc gggacctcca cgtgtttcag ccctagcacg cggagcactt
     3541 ggagtgctcg gcgatcgagt tcgttccgat gctctggttc ctatgggttt cggcccccca
     3601 gaaccccgaa accgcggccg cgtagctact tccgagaggg
//
LOCUS       DASTKS010000061         6076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1792393, whole genome shotgun sequence.
ACCESSION   DASTKS010000061 DASTKS010000000
VERSION     DASTKS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792393"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFQ35_01300"
     CDS             <1..785
                     /locus_tag="VFQ35_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929285.1"
                     /translation="DANGKGDYQSCSEFNPALVLSKEEQKTLSQEDRDHENAASRRVV
                     LYIFERDTQIDLAKWPCPTVKEGTAGCRKRFWSDADTRRAPGDVRRSFDATADTFACR
                     FYQRFAGGSPCERPTGLTLFMEIYFDLATTQMVAPQAFTLSSSDSSFLQTLTTEATIR
                     VDEATLLLEFPGVRPGKSYSLSYQLADGSVVPIFTDVPFEGVDDFGSDTPEPKAVELA
                     QAEEPEPRVNEGADEPLLAFNSVDVDLDPSLTGDPNSGTAVA"
     gene            787..3108
                     /locus_tag="VFQ35_01305"
     CDS             787..3108
                     /locus_tag="VFQ35_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929286.1"
                     /translation="MSTIISRPFRFTEFQLFDAPSNDENGWKPVALPDNRPPPRLTGK
                     FVYAFQVLGADTQLFAEILADAQGKLAVTPGRVARTTERDKRAAPAATNTDVFTLPHT
                     INGKRAFYFFLVSRIRLHVSTINDLEVTRPPALVPLDLEKDGSFLLVGGSDGNSTTIE
                     TFLPVIDPITIGINLHTRYEQACNELIAFTMEFKEQSDAQRTRVRDRHKALFLAKLLQ
                     SVVEGDPSDKLGLKNEFKNDDPSAPKKFIETYDKTVEDLVKRRDRLVEPLVHFLAGKL
                     LETAENSYLVTEKDDYAAFLSHLEVMYDRINEAPLGKARIGKLIDNPTFAFREYVLRT
                     SPAPDAKFSVGRKANSAILGLWKEAVPGVLSKAPNAAEKIANALKFIARIELVKVEAK
                     EISYTYRKKNGRLRTVTKTVQETTAIAPATALAKWAQDGLPGRGLERLAQGIEIANLT
                     LALVSLANAEPGEDRGFALLNLFGSVLDGVSAFNAFLKIGERSIKIVGGVSAAVDTVL
                     AARDGINAYGRDDTSSAVGFGIVATGSVLTLAGCVCAAAGLAAGATVVGIPLAAFLEV
                     VGAVLVGAGWILAVFSADSEIELFVSHCMFGKNSNSGSKDKPKWASAAFGTWSQPGGL
                     DHQVKSLLTILAAFTVKATDYTGINVQLGLTMSGSKLRIKFDAGYNLGITHRPDLLID
                     IGQQSMQQFGGDPQDPSRLRVGESNGRPFVQITADWPPNVRPPKAIQHQYCMVEVQLL
                     LDDQGNTVPIQKALSYRVHELNSILSGPLSSHE"
     gene            complement(3199..3669)
                     /locus_tag="VFQ35_01310"
     CDS             complement(3199..3669)
                     /locus_tag="VFQ35_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929287.1"
                     /translation="MLSSSTPLRSAIRPSIRLAVLLLLAAGCARTRTASEQASPRPSN
                     EPQADICAALRQPGSLRQRFEKQLPLRVGGVVWALQGAQASDVERAFASLELSGTRVV
                     RCEAGEVVAWAPGVDDGAVQGLEAAMRSSAANLKVLDREVMPPEAAAAPKPKRD"
     gene            complement(3670..4317)
                     /locus_tag="VFQ35_01315"
     CDS             complement(3670..4317)
                     /locus_tag="VFQ35_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9929288.1"
                     /translation="MTATHTVREGEHLGTIAKKYGFVNFSVLWEHPENASLKELRKDP
                     MLLAPGDKVFIPDRVQLIFQRATHDSYDFKVRRDKLSLILKFFDFGGTPRKNTPVALN
                     VLAPDTGAASSGPPQTTDGDGSVSIDIATHVNTGTLELDGVELPLHIGALDPIETENG
                     VAQRLRNLGYPVPASPKKEPQQMRLALEEFQADYGQKVTGERADVEPKLKEIYGG"
     gene            complement(4318..5517)
                     /locus_tag="VFQ35_01320"
     CDS             complement(4318..5517)
                     /locus_tag="VFQ35_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929289.1"
                     /translation="MSSPLFSPITHYKDAPVVALATEFVSATPPRGTWPTQFGGGARI
                     SRIDEPLLQDTWRVRWQVELGDAERPRGLVLGEGRILVNGSERRGVWLLDGTPVGFFK
                     RNPGAAFLDAEGKHFLCADSGGIATYSLDGVREALLPLSLATFATVREVLAGPSALVF
                     VSNQKPPQASALLLAECMRLVDYGYRENGLLYGVEPVAGVMREGEHEIAATASDTGPV
                     LATSDGVCFLDWHLSIRHEWPLSTIPLAVSVDAAGRALLVSDDYGELKFRVLPPHPTD
                     PEREPLVELTLPSECRGAIPPLVTQNGQIYVCSEHALLALAPTGQLSWQQARSSLAPP
                     VLTQNGLLLMADDQLYAVVSDGTRVPLWRPPHRIRTEAILADGIIYVATDSELFALEP
                     AHAATEG"
     gene            complement(5514..>6076)
                     /locus_tag="VFQ35_01325"
     CDS             complement(5514..>6076)
                     /locus_tag="VFQ35_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929290.1"
                     /translation="SCRGATHLVASFVTWREASVAKSRALLVPLPCPPTGEASPAAGC
                     IGAGLTGAERRARARDSAKRLHADSEETVLTPGDGLSIYALAPDDVLAVDSLRRLRAS
                     DFALRAQVDWLKSQYVFEHKPGEELPALVLISRERAPRPRVFDRSLANEYCVPDPGAT
                     PGFAYCYPEHFIVDWQGEWKPLHRTP"
BASE COUNT         1129 a         2000 c         1914 g         1033 t
ORIGIN      
        1 ctgatgcgaa cggcaagggc gactaccaga gctgcagcga gttcaaccct gccctcgttc
       61 tctcgaaaga agagcaaaaa acgctcagcc aagaagatcg tgaccacgag aacgccgcga
      121 gccgccgcgt cgtgctctac attttcgagc gcgacacgca gatcgatctc gccaagtggc
      181 cctgcccgac cgtgaaggaa ggaaccgccg ggtgccgcaa acgcttttgg tccgacgccg
      241 atactcgccg agctccgggc gacgtccgtc gcagcttcga cgccaccgcg gacacgttcg
      301 cctgccgatt ttatcaacgc tttgccggtg gctctccgtg tgagcgcccg actggcctca
      361 cgctgttcat ggagatctat ttcgatttgg cgacgactca gatggtggcg ccacaagcgt
      421 tcacgttgtc gagtagcgac tcgagcttct tgcagacgct caccactgaa gcgaccattc
      481 gcgtcgacga ggccacgttg ctgctggagt ttcccggcgt acgccccggc aagtcgtatt
      541 cgctctcgta tcagctcgcg gacggcagcg tcgttcccat cttcaccgat gtgccgttcg
      601 agggcgtcga cgacttcggc agtgacacac ctgagccgaa ggccgtggag ctcgcgcaag
      661 ccgaagaacc agagccccgc gtgaacgagg gcgcggacga gccgctgctc gctttcaact
      721 cagtcgacgt cgatttggac ccttcgctca cgggcgaccc gaactcggga acggcggtgg
      781 cctgagatgt cgacgatcat cagccgcccc tttcgcttca ccgagtttca gctcttcgac
      841 gcgccgtcga acgacgaaaa cggctggaaa cccgtcgcgc tgccggacaa tcgcccgccc
      901 ccgcgcctca ccggcaagtt cgtttacgct ttccaagtcc tcggcgctga cacgcagctc
      961 ttcgccgaga ttttggcgga tgcgcaagga aagctcgcgg tgacgcccgg ccgcgtcgcg
     1021 cgcacgaccg agcgcgacaa gcgtgccgcg cccgccgcca ccaacacgga cgtcttcacg
     1081 ctgccgcaca ccatcaacgg caagcgcgcc ttctacttct ttcttgtgag tcgaattcgg
     1141 ctccacgtga gcacgatcaa cgatctcgaa gtcacgcggc cgcccgcgct cgtcccgctc
     1201 gatctcgaaa aggacggctc gtttttgctg gtcggcggca gcgacggaaa ctcgacgacg
     1261 atcgagacat ttttgccggt gatcgatccg atcacgatcg gaatcaactt acacactcgc
     1321 tacgagcaag cctgcaacga gctcatcgcc ttcacgatgg agttcaagga gcagagcgac
     1381 gcgcagcgca cgcgggtgcg cgaccgtcac aaggccctgt tcttggcgaa gctcctccaa
     1441 tcggtcgtcg aaggcgaccc gagcgacaag ctggggctga agaacgagtt caaaaacgac
     1501 gatccctcgg cgcccaagaa gttcatcgag acctacgaca agacggtcga agatctggtg
     1561 aaacgccggg accgcctcgt ggagccgctc gtgcacttct tggccggcaa actgctcgaa
     1621 acggccgaaa actcgtacct ggtcaccgag aaggacgatt acgcggcgtt cttgagccac
     1681 ctcgaggtga tgtacgaccg catcaacgag gcccccctcg gcaaggcgcg catcggtaag
     1741 ctcatcgata acccgacgtt cgccttccgc gaatacgtgc tgcgaacctc ccccgccccg
     1801 gacgcgaagt tctcggtcgg gcgcaaggcg aactcggcga tcctggggct ttggaaagag
     1861 gccgtccccg gcgtgctcag caaagctccg aacgccgcgg agaagatcgc gaacgcactc
     1921 aagttcatcg cgcgcatcga gctcgtcaag gtcgaggcga aagagatcag ctacacgtac
     1981 cgaaagaaga acgggcggct tcggacggtt accaagaccg tgcaagagac caccgcgatc
     2041 gccccggcga cggcgctcgc caagtgggca caggacggat tgcccggtcg tggcctcgag
     2101 cggttggcgc agggcatcga gatcgcgaac ttgacgctcg cgctcgtgag ccttgcgaac
     2161 gccgagcccg gcgaggacag aggctttgct ctactgaacc tgtttggctc ggtgctggac
     2221 ggggttagtg ccttcaacgc ctttttgaag attggcgagc gcagcatcaa aatcgtgggc
     2281 ggagtctcgg ctgcggtcga cacggtgctc gccgcgcgag acggcatcaa cgcctacggt
     2341 cgggacgata cgagcagcgc cgtcggcttc ggcatcgtcg ctaccggctc cgtgctcacg
     2401 ctcgcgggct gcgtgtgcgc ggcggcgggt ctcgcggcgg gcgcaaccgt ggtcggcatt
     2461 ccgctcgcgg catttctcga ggtggtcggc gccgtgctcg tcggagccgg ctggattttg
     2521 gccgtgttca gcgccgattc cgagatcgag ctcttcgtga gtcactgcat gttcggcaag
     2581 aacagcaact cgggctcgaa agacaaaccc aagtgggcgt ccgcggcgtt cggaacctgg
     2641 tctcaaccgg gcgggctgga tcaccaagtc aagtcgctgc tcacgatcct cgccgcgttc
     2701 accgtgaagg ccaccgacta caccggcatc aacgtgcagc tcggcctcac catgtcggga
     2761 tccaagctgc gaatcaagtt cgacgccggc tacaacctcg gcatcacgca ccggcccgat
     2821 ttgctcatcg atatcggaca acaatcgatg caacagttcg gcggcgatcc acaagatccg
     2881 agccggctcc gtgtcggcga gagcaatgga cgacccttcg tgcagatcac ggcggattgg
     2941 ccgccgaacg ttcgcccgcc gaaggcaatc cagcaccaat actgcatggt cgaggtgcag
     3001 cttctactcg acgatcaggg aaacaccgtg ccgattcaga aggccctgtc ctaccgagtt
     3061 cacgagctga actcgattct gagcggccct ctatcgagtc acgaatgacg cgcgccgctc
     3121 gcgggcaggc tccacgcagc cgcgaatcaa gcgcaccgct cgcgcgcatc agcgagcgcg
     3181 cttccgacct tcgctcgctc agtcgcgttt cggcttgggc gcggcggcgg cctcgggcgg
     3241 catgacctca cgatccagga ctttcaggtt ggccgcgctg gagcgcatgg ccgcctcgag
     3301 cccctgaacg gcgccgtcgt ccactccggg cgcccacgct acgacctccc ccgcctcaca
     3361 ccgaacgaca cgcgtgccgc tgagctcgag tgaggcaaag gcgcgttcga cgtcgctcgc
     3421 ctgcgcgcct tgaagcgccc aaacgacacc gccgactcgg agcggcagct gcttctcgaa
     3481 gcgctggcgc aacgaccccg gttgacggag cgcggcgcaa atgtcggctt ggggttcgtt
     3541 gctcgggcgc ggcgacgcct gctccgaagc ggttcgggtg cgggcgcagc ccgcggcgag
     3601 caggagcagc acggcaaggc gaatcgacgg acggatggcg ctccggagcg gagtcgacga
     3661 gctcagcatt cagcctccgt agatctcttt cagcttcggc tccacgtcgg cgcgctcgcc
     3721 ggtgaccttt tggccgtaat cggcttggaa ttcctcgagc gcgagacgca tttgctgcgg
     3781 ctcctttttc ggcgaggccg ggactgggta gccgaggtta cgcagccgct gggcgacgcc
     3841 gttttcggtc tcgattgggt cgagcgcgcc gatgtgcagc ggtagttcca ctccgtcgag
     3901 ctcgagcgtc ccggtgttca cgtgggtcgc gatgtcgatg ctgacgctcc cgtcaccatc
     3961 cgtcgtctgc ggcggccccg agctcgcggc gccggtgtcc ggtgcgagca cgttcagcgc
     4021 gacaggcgta ttctttcgcg gtgttccccc aaaatcaaaa aacttcaaaa tcaagctcag
     4081 cttgtcgcga cgtactttga agtcgtagct gtcgtgcgta gcgcgctgga aaatcagctg
     4141 cacgcggtcg gggatgaata ctttgtcccc tggcgcgagc agcatcggat ctttgcgcag
     4201 ctctttgagc gatgcgttct ccggatgctc ccacagcacc gaaaagttca cgaagccgta
     4261 cttcttcgcg atggttccga ggtgctcacc ttcccgaacg gtgtgcgtcg cggtcactca
     4321 accctccgtc gcggcgtgcg ccggctcgag cgcgaagagc tccgagtcgg tcgccacgta
     4381 aatgatccca tccgcgagga tcgcctcggt gcggatccga tgcggaggcc tccacagcgg
     4441 aacgcgcgtg ccatccgaca cgacggcgta aagttggtcg tccgccatca atagaagccc
     4501 attttgcgtc aacaccggag gcgcgagcga tgagcgggcc tgctgccacg agagctgtcc
     4561 ggtcggtgcg agcgcaagga gcgcgtgctc cgaacacaca tagatctgcc cattttgcgt
     4621 gacgagtggt gggatcgctc cgcggcactc ggacgggagc gtgagctcga cgagcggctc
     4681 gcgctcggga tccgtcggat gcggaggtaa gacgcgaaac ttgagctcac cgtagtcgtc
     4741 cgagacgagc agcgcacgac ccgccgcatc caccgacacc gcgagcggaa tggtcgagag
     4801 gggccactcg tgccgaatgg acaaatgcca gtcgaggaag caaacgccgt cgctcgttgc
     4861 gagcactgga cccgtgtcgg aagcagtcgc ggcgatctcg tgctcgccct cgcgcatcac
     4921 gccggcaacg ggctcgactc cataaaggag gccgttttcg cggtagccat agtcgacgag
     4981 gcgcatgcac tccgcaagca agagcgcgct tgcttgcggc ggcttctggt tcgaaacgaa
     5041 cacgagcgca gacggcccgg ccaagacttc gcgcacggtc gcgaacgtcg cgagtgagag
     5101 cggcagcaaa gcttcgcgaa cgccgtcgag cgaatacgtg gcgatgccgc cggagtcggc
     5161 gcacaaaaaa tgctttccct ccgcatccag gaaggccgct ccggggttcc gcttgaagaa
     5221 gccgacgggc gtgccgtcga ggagccacac gccgcgccgc tcgctgccgt tcacgagaat
     5281 gcggccctca ccaaggacga gcccccgtgg gcgctccgcg tcgccgagct cgacctgcca
     5341 gcgcacgcgc caggtgtctt gtagcagcgg ctcgtcgatg cgactgatgc gtgcgccccc
     5401 gccaaattgc gtcggccacg tcccgcgcgg aggcgtcgcg ctgacgaatt cggtcgcgag
     5461 cgcgacgact ggcgcgtcct tgtagtgcgt gattggcgaa aacaggggcg agctcatggg
     5521 gtccgatgga gcggtttcca ttcgccctgc cagtccacga tgaagtgctc ggggtagcag
     5581 taggcgaagc caggcgtggc gccagggtcc ggcacacaat attcgtttgc gaggctacgg
     5641 tcgaatacgc gcggacgcgg cgccctctct cgagaaatca gaaccagagc cggcagctct
     5701 tccccgggtt tgtgctcgaa tacgtattgc gacttcagcc aatccacctg cgcgcggagc
     5761 gcgaagtccg acgctcgcag ccttcgcaac gaatcgaccg cgagtacgtc gtcgggcgcg
     5821 agtgcataaa tgctgagccc gtcgccgggc gtgagcaccg tctcctcgct gtcggcatgg
     5881 aggcgcttgg ccgaatcgcg cgctcgcgct cggcgttccg cgccggtgag cccggcacca
     5941 atgcaacctg cagccggcga cgcttccccc gtgggagggc acggcaacgg aacgaggagc
     6001 gctcgggact tcgccaccga cgcttccctc cacgtcacga acgacgccac caaatgcgtt
     6061 gcgcctcgac acgacg
//
LOCUS       DASTKS010000062         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_900435, whole genome shotgun sequence.
ACCESSION   DASTKS010000062 DASTKS010000000
VERSION     DASTKS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900435"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFQ35_01330"
     CDS             <1..616
                     /locus_tag="VFQ35_01330"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HET9929291.1"
                     /translation="APNLAGTRACPSGYEEGGPWRQVSRLGLPLINEAVIGIQDKDKY
                     NRTTPANDVANFGAYFLNPILVRDVEAIGGYAQLGIDTVPDSLKYGRVDILQVINLND
                     IPSAGAHSVPIENGRTGDVLRVDVAVPSSFPNGRALSGPAVASGKEEDVTDTELSLLL
                     TQLTAKVTDYVSGPGDGRKLLPYFPYLATPFAGDIEGKGSPAKF"
     gene            1826..3940
                     /locus_tag="VFQ35_01335"
     CDS             1826..3940
                     /locus_tag="VFQ35_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9929292.1"
                     /translation="MFAGALCGAAMLTAPLARAEETTTATPEVVVTGTRPADAASERD
                     VGRDELAARPVRAAADLLRATPGLVTAQHSGGGKANQYFLRGFDADHGTDVALSFDGV
                     PINMVSHGHGQGYADTNFIIPEVVERLEIRKGPYFADLGDFATAGALNLVSRASVPHA
                     SMGVGFEASPGHGSPSYRALMLASPKLGSARALLAADIARENGPFQHPNHWDRYRLFD
                     KISFKSGKSALVTLSFAGYAGSWNGSGQLPARAVEDGYVSRFGSLDPSEGGASARHQA
                     SLTYRALLDGDAEFKALAYAARYRFNLFSNFTLYLDDPTLGDQIEQQDRRTILGAKLS
                     YRRPTRVFGMPFETTLGAEARRDDIEAGLWKSYQRQRVALRTRHEVNEQLLSLYVNER
                     WAPASFVRLDVGVRADWLVFDVTDRSSGAAGPKSDTAAQLSPKAGAVFDLFRKRELEL
                     QAFANYGRGFHSNDVRGAFASPRVSPLARAVGAELGARTRLFGRAELSMALFQLDSAS
                     ETVWSGDAGTTEAAGASRRQGVELMARYGLSDWLTADLDVTLTRARFRDGSEVPLAPR
                     QTWAGGLTARRDLGEGRARAALRFFGVGERPASDDGVLSATGFTELDLALGYTLRRFD
                     FALDVENLLNSEFRSAQFATVSRLANDPAVGQAVPPGFSCGRNARLAPSGASGGFGGC
                     EDVHFTPANPLTVRVMATVFLD"
     gene            3944..4507
                     /locus_tag="VFQ35_01340"
     CDS             3944..4507
                     /locus_tag="VFQ35_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929293.1"
                     /translation="MSASVTSPSDVELVQRMARGDRDALGALYERHAPRLSTLAQHIL
                     GRSNEAEDLLHDVFLEAWRHAANYTEARGSVWAWLCVRTRSRAIDRRRASPRNVSLEG
                     FPDRSPLEELVEGDLELSPERDKLRSALLEMSREEREVLALGYFEGMSSSEIADAIRI
                     PVGTVKSRTRSALAKLRERLLGKGGER"
     gene            4507..5079
                     /locus_tag="VFQ35_01345"
     CDS             4507..5079
                     /locus_tag="VFQ35_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9929294.1"
                     /translation="MQDLDWEDPWRDADIDAEALGSSLAGLLQPKPALAREELLRAVA
                     AFPLRYAPFFSRIAELWDLPVGDVEAVMQRAREPSNWRKPGLPGLTLIDVEGGERVRG
                     AQVNLVRFAAGMRFPAHSHPGPETLLILEGSYRDSEGVHVGPGDIHSMLPGSEHSFRV
                     GREGPCVAASVQYGRRFTGLVMRALIRLFG"
     gene            complement(5154..5564)
                     /locus_tag="VFQ35_01350"
     CDS             complement(5154..5564)
                     /locus_tag="VFQ35_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929295.1"
                     /translation="MSLPQPVSEALSPFYGASPRAVHRAKVELAKLVLAEAPPQAVRQ
                     LELAERFLSGEANDAELATEKTEVWTYIGSLACYCTVSDSASAQAVLSCLESEDRHHT
                     IESLFEQIERVSRCRVDAARIMSALRHAAEHLRC"
BASE COUNT          923 a         1884 c         1959 g          995 t
ORIGIN      
        1 cgcgccgaac ctcgccggta cccgcgcttg cccgagcggt tacgaagagg gcgggccgtg
       61 gcgtcaagtg tcgcgcctcg gcttgccgct catcaacgaa gccgtgatcg gcatccagga
      121 caaggacaag tacaaccgca cgacccccgc gaacgacgtc gccaatttcg gcgcgtactt
      181 cctgaacccg atcctcgtgc gggacgtcga agccatcggc ggttacgccc agctcggcat
      241 cgacaccgtg cccgattcgc tgaagtacgg ccgggtcgac atcctccagg tcatcaacct
      301 gaacgacatc ccgagcgccg gcgcgcactc cgtgccgatc gaaaacgggc gcaccggcga
      361 cgtgctgcgc gtcgacgtag cggtcccgtc ttcgttcccg aatggccgcg cactgagcgg
      421 gccagcggtt gcgtcgggca aggaagagga tgtcaccgac acggagctct cgctccttct
      481 cacccagctc acggcgaagg tgaccgacta cgtgagcggc cccggtgacg gccgcaagct
      541 gctgccctac ttcccctatc tcgcgacgcc gttcgcgggc gatatcgagg ggaagggcag
      601 cccggcaaag ttctgagccg tcgataagtg aggcgaggga agcggccggc ggcgtgagtc
      661 gtcggccgtt ttgccttggg gtcagtaagc gaggggaggg aagcggccgg cggcgtgagt
      721 cgtcggccgt tttgccttgg ggtcagtgtt gatcgcgagg gcgcagcgga agttgcagcg
      781 tcggtgtcgc gctcgagcgc agcgcggcca agctggccct gcatttcatg ccgcagcggc
      841 gggcgccatc gggctgttcg acgcatccgc cggcggagca atcgaggtca ctttcgcagc
      901 tctccccggc gcgggcttgg ggaaggcagc gctctttcac gcagcgcagc ccatcggcgc
      961 aaccagcctt gcagctttcc ccttcttgcc ccgcgcttgc gacgacgcac tggccctcga
     1021 tgcaacgttg agcttggccg caattcgcgc tggagtagca ggccgagcca actttgggcg
     1081 cctcgccaca acgatgctcc gagaagatgc agcgttcgcg gcaagtcggg tgcttgcgct
     1141 cagagggacc ctgccgtgtg taagtcgcga gggcgtcgac gctcgctccg cacgcttcgc
     1201 cgatttcacc cggtgcgcgg cacttgccga gcccgtgaat gctggcgcgg tcgcagtgaa
     1261 gtggctctcg gcactcgaga gaggacccgc acggttcgcc ctcagcgcgc ccgccgctga
     1321 ggaccccttc gcattctttc ggggttgctt ggctccacgg cacgacccag tcgcagccgc
     1381 tggtcgcccg tagcatcgcg ctacggcagg ccgaaaggcg ctctctgtcg atttgcgcgc
     1441 ctcgcgtcag agcggccccg acctcgacgc ggcaaatctc ggcgaggccg agctcggtcg
     1501 cgcccccgca gcacgcggcc ttccgcgtgg ccggaagctc gtggagcgtc tcgcaaagct
     1561 cacgggctag ctcgaggctc gcgaggcttt cccgtttgct ggcggcggaa cccgaggcga
     1621 gcggcgcggt aggcaccgct tgctcgcgct tgcaggcaga tgcaacggtt agggcaacta
     1681 ggctcgcgag caaagctctg cgagtaccgc gcccgaaatc caggtatgag gctgtctcgt
     1741 aagaggtgaa cggcacctga ctgctacgat gctcgaccct acctggatct actcggaaag
     1801 gcgacttccg cgaagcggag cggcgatgtt cgcgggcgcc ctctgcggcg cggcgatgct
     1861 cacggcgccg ctcgcccgcg cggaggagac cacgaccgct acgccggaag tcgtggttac
     1921 ggggacgcga ccggcggacg ccgcgtccga gcgcgatgtc ggtcgcgatg agctcgcggc
     1981 gcggccggtg cgcgcggccg cggatttgct ccgcgcgaca cctggactcg tcaccgcgca
     2041 gcattcgggg ggcggcaaag cgaatcagta tttcctgcgc ggcttcgacg cggatcacgg
     2101 cacggacgtc gcgctctcgt tcgatggcgt tcccatcaac atggtctcgc acggccacgg
     2161 ccaaggctac gcggacacga acttcatcat ccccgaggtc gtcgagcgcc tcgagattcg
     2221 caaggggcca tacttcgccg acctcggcga ctttgctacg gcgggtgcac tcaacttggt
     2281 gtcgcgcgcg agtgttcccc acgcatcgat gggggttggg ttcgaggcgt cgccggggca
     2341 tggatctccc agctaccggg ctctcatgtt ggcaagcccc aagctcggtt cggcgcgagc
     2401 gctgctcgct gccgacatcg cgcgtgaaaa cgggcctttc cagcatccga accactggga
     2461 tcggtatcgg ctgttcgaca aaatttcgtt caagtccggc aaatcggcgc tggtcacgct
     2521 gagcttcgcc ggttacgcgg gcagctggaa cggctccggg cagctgccgg cgcgcgccgt
     2581 cgaagacgga tacgtgtcgc gcttcgggtc gctcgatccg agcgaagggg gggccagcgc
     2641 tcgtcaccaa gcgtcgctga cctaccgagc tctgctcgat ggcgacgcgg agttcaaggc
     2701 gctcgcgtac gccgctcgtt accggttcaa tctgttctcg aacttcactc tctacctcga
     2761 cgatcccacc ctcggtgacc aaatcgagca gcaagaccgg cgcacgattt tgggcgcgaa
     2821 gctcagctac cggaggccga cgcgggtctt cggcatgccg ttcgagacca cgctcggcgc
     2881 cgaggcgcgt cgggacgaca tcgaggcggg actctggaag agctatcagc gccagcgcgt
     2941 cgcgcttcga acgcgccatg aagtgaacga gcagctcctt tcgctgtacg tgaacgagcg
     3001 ctgggctccg gcgtcgttcg tccgcctcga cgtcggtgtt cgcgcagatt ggctcgtttt
     3061 cgacgtcaca gatcgcagct ctggtgccgc tggcccgaag agcgataccg cggcgcagct
     3121 gagcccgaag gcgggcgctg tcttcgacct gtttcgaaag cgtgaactcg agctccaggc
     3181 gttcgcgaac tatggtcggg gttttcactc gaacgacgtg agaggcgcgt tcgcgtcgcc
     3241 gcgggtctcg ccgctcgcgc gcgccgtcgg tgccgagctc ggggcgcgga cgcggttgtt
     3301 cggtcgcgcc gagctctcga tggcgctctt ccaactcgac tcggcgagcg agaccgtttg
     3361 gagcggtgac gccggcacga ccgaagcggc cggagcgagc cggcgacagg gcgtcgaact
     3421 catggctcgc tacgggctca gtgattggct gaccgcagac ttggacgtga cgctcacgcg
     3481 cgctcgcttc cgagacggga gcgaggttcc gctcgcgcct cggcagacgt gggcgggagg
     3541 gcttacggct cgaagagatt tgggcgaagg tcgcgcccgt gccgcgctgc gctttttcgg
     3601 tgtcggcgag cgtcccgcga gcgacgatgg ggttctctcc gcgaccgggt tcaccgagct
     3661 cgacctcgcg ctcggctaca cgctccggcg cttcgatttc gcgctcgacg tcgagaacct
     3721 gctgaactct gagtttcgct ccgctcagtt cgcgaccgtt tcgcggctcg cgaacgatcc
     3781 tgccgtcggg caagcggttc cgccgggctt tagttgcggc cgcaacgcgc ggctcgcgcc
     3841 cagcggcgcc agcggaggtt tcggtggttg cgaggacgtg cactttacgc ccgccaaccc
     3901 gctcaccgtg cgcgtcatgg cgaccgtatt cttggattga gccgtgtcgg ccagcgtcac
     3961 ttctcccagc gacgtcgagc tcgtgcagcg catggcgcgt ggagaccgcg acgcgctcgg
     4021 ggcgctctac gagaggcacg cgccacgtct gtcgacgttg gcccagcaca ttttggggag
     4081 gtcgaacgaa gccgaagatc tgctgcacga cgtgttcctc gaagcatggc gacacgccgc
     4141 gaactacacg gaggctcgcg gcagcgtgtg ggcgtggctc tgcgtccgca cccgtagccg
     4201 cgccatcgac cgccggcgtg cgtcgccccg caacgtgagt ctggaaggct ttccggatcg
     4261 ctcgccgctc gaggagctcg tcgagggtga tctcgagctc tcccccgagc gcgacaagct
     4321 gaggagtgct ctgctcgaaa tgtcgcgtga ggagcgcgaa gtgctcgcgc tcgggtactt
     4381 cgaaggcatg agcagcagcg agatcgccga cgcgatccgg ataccggtcg gaaccgtgaa
     4441 gtcgaggacg cgatcggcac tggcaaagct tcgggaacga ctgctcggga aagggggcga
     4501 gcgctgatgc aagacctcga ttgggaagat ccttggcgag atgcagacat cgacgccgaa
     4561 gcgctaggaa gctcgctcgc agggctgctc caaccgaaac ctgccctcgc gcgggaggaa
     4621 ctgctccgag cggtcgccgc gttcccgctg cgctatgcgc cgttcttctc ccgcatcgcc
     4681 gagctctggg atttgcctgt cggtgacgtc gaggcggtga tgcaacgcgc gcgagagccc
     4741 tcgaactgga gaaagccagg gttaccgggg ctcacgctca tcgacgtgga gggcggagag
     4801 cgagtccgtg gcgctcaagt gaacctggtt cggttcgcgg cggggatgcg ctttcccgca
     4861 cattcgcatc ccgggcccga gacgctgctg attctcgagg gatcctaccg cgactcggaa
     4921 ggcgttcacg ttgggccagg ggacatccat tccatgctgc caggcagcga acactcgttt
     4981 cgagtcgggc gcgaggggcc ctgcgtggcc gcctcggttc aatacggtcg caggttcacg
     5041 gggctcgtca tgagagctct gatcaggctg ttcggatgac gccggccgtc ccgtcagatc
     5101 gctgagaaag tcggcctgac cccccccatg catccgcgtc gaaaacgacg ctctcaacat
     5161 cggaggtgct cagcggcgtg ccgcaacgca gacatgatgc gcgcagcgtc gacgcggcag
     5221 cgcgagactc tctcgatctg ctcgaagagc gactcgatgg tgtggtggcg atcttcgctc
     5281 tcgaggcagc tcaggaccgc ctgcgctgaa gccgagtccg aaacggtgca gtagcaggcg
     5341 agcgagccga tgtacgtcca gacctccgtc ttctcggtcg cgagctccgc gtcgttggcc
     5401 tccccggaca ggaatcgctc ggcgagttcg agctggcgta cagcctgcgg aggcgcttcg
     5461 gccagaacga gctttgcgag ctcgaccttg gcgcggtgca ccgctcgcgg ggatgccccg
     5521 tagaaagggc tcagggcttc actgaccggt tgaggaaggc tcattgggat cgagccgcgc
     5581 cactagcacg agagctcgag atggaggtcc accccgctcg cttttgtagg cgctggcttc
     5641 tcgattttgc cacacgcgga gcctgcgacg gtgcatgggc gcgacgaacg gtgccaactc
     5701 tctcaagaat tcgtgcaccc tgcggcggcg ctggctgata tcgacggcct ccgtcgccac
     5761 c
//
LOCUS       DASTKS010000063         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_231442, whole genome shotgun sequence.
ACCESSION   DASTKS010000063 DASTKS010000000
VERSION     DASTKS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231442"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..567)
                     /locus_tag="VFQ35_01355"
     CDS             complement(109..567)
                     /locus_tag="VFQ35_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929296.1"
                     /translation="MAREPRHSPRRSSPRQRPAAQPASFMIVVESGAHWPSWMSDTVG
                     KSPRRVLAQLDGEDCAAFAERVARGGKVPGELVALVCNQRADGRQMDARRRALANRSA
                     RAWLVVGPNTRDELLEQLVRLVSELPDTELRAGSASPLPPSERAPLSSVA"
     gene            651..1766
                     /locus_tag="VFQ35_01360"
     CDS             651..1766
                     /locus_tag="VFQ35_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929297.1"
                     /translation="MNNLRALGRPVSFALVLALVSASVPAFAQTDEQRAAARALATEG
                     ATAFNEGRYKDAADLFGRAESLVHAPPHLLFLARSFAKLGQFVRARETYIRITKEPVA
                     ANAPQAFRDAQSAAQEELKGVEPKIAKLTIQIANAQDAQNLAVTVDGNPVPPVLVGVP
                     QPVDPGDHRVEATAVGKRSRPQMVSLRDGERGAVNLVLESDAGAAGAPLPGTPAATPG
                     TPAATPATPGLGPAPMPPPAPAPAEQSSGNNGMRIGSYVGFGVGAVGLGLGTLFLLKG
                     ASKRSEANDAFDACKSDLNCRDLPATSPKKAKVDDLDSQATKAQRVGVAGMVVGGLGV
                     AAGVTLLVLSGKSSTETKTGRLVPIVSPNYIGVSGAF"
     gene            2011..3486
                     /locus_tag="VFQ35_01365"
     CDS             2011..3486
                     /locus_tag="VFQ35_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9929298.1"
                     /translation="MALEIGQLIENKYRITRLIGEGGMGAVYEGENIRINRRVAIKVL
                     HSAFTGNQEVMHRFEREAQAAGRIGNDHILEVLDLGSLNDGDHYIVMEFLDGEPLSNR
                     IKNRGRLSPQELTPILRQVLVGLGAAHVAGIVHRDLKPDNIFILREKAGTRDFVKIID
                     FGISKFQPLSGDGMKMTRTGAVMGTPYYMSPEQASGSHEADQRSDIYSMGVIMFEAVT
                     GKVPFDAATFNQLMFKIVLAEVPTVESVVPDIDPAYSSIIAKAMARDLTHRFQNIEEF
                     IRALDEWTARGTAVSVPPPNNAEDAGLVPRAHGRPAMGSHAGLEAGPVGARTAGNWAT
                     SQPGAEEIAGLPKKGSSSIIAIAAAAAIVVLGGGAFAAYSALGKGQTAAQSAPSDAKP
                     AAAAQAPAAPTAPPAAVTPSPAAPEVKPAEVKPAEVKPAEAKPAEVKPSGVKPTPSVA
                     AAPVAARPVAAAPRPQLPAQRPPARPKPEKSKPSASPDFGY"
BASE COUNT          757 a         1437 c         1424 g          721 t
ORIGIN      
        1 gcgaagacat cgcgcgctcg gcgcacgctc acccgacgct cgccgaggcg atgaaagaag
       61 ccgccctcgc agtgcaaggt cggccgattc acatctgacg cggcgagctc aggccacgct
      121 cgagagcggc gctcgctccg aaggggggag cggagacgcg ctacccgcgc gaagctcggt
      181 gtctgggagt tcgctcacga ggcgcacgag ctgctcgagc agctcatcgc gcgtgttcgg
      241 accgacgacg agccaggcgc gagcgctgcg gttcgcgagc gcgcgccgtc gcgcgtccat
      301 ttgccggccg tcggcgcgct gattgcacac cagcgcgacg agctctccgg gcacttttcc
      361 gccgcgcgcg acgcgctcag cgaaggccgc gcagtcctcg ccgtccaact gggcgagaac
      421 gcgtcgcggt gactttccga cagtgtccga catccagctc ggccagtgcg cgcccgattc
      481 gaccacgatc atgaacgagg cgggctgcgc ggcggggcgc tgacgaggag acgaacgacg
      541 cggagaatgg cgaggttcac gagccatgac gctgtcgtat cggacgcgcg ttcggagggc
      601 caagaaatgc ggcgcgcggg gagccggttg gtctaggatg acccgttgtc atgaacaact
      661 tgcgagcttt gggtcgcccg gtgagtttcg cgctggtgct tgctttggtg tctgcctcgg
      721 tgccggcatt cgcacagacc gacgaacagc gagccgccgc gcgtgcgctc gcgaccgagg
      781 gcgccaccgc cttcaatgaa ggccgttaca aagacgcggc agacctcttt ggtcgcgccg
      841 aatctttggt tcacgctccg ccccaccttt tgttcttggc tcgctcgttc gcgaagctcg
      901 gacagttcgt gcgcgctcgc gagacgtaca tccgcatcac gaaagagccg gtagccgcaa
      961 acgctccgca agcgttccgt gatgcgcagt ccgcggcgca agaggagctg aagggcgtcg
     1021 aacccaagat cgcgaagctt acgattcaaa tcgcgaacgc tcaggacgca cagaacctcg
     1081 ccgtaaccgt ggacggcaac ccggttcccc cggtgctcgt cggcgtgccg cagccagtcg
     1141 atcccggcga ccaccgcgtc gaggcgactg cggttgggaa gcggtcacgt ccgcagatgg
     1201 tttctttgcg cgatggtgag cgtggcgcgg tgaacttggt gctcgagagc gatgctggtg
     1261 ccgccggtgc gccgctgcca ggtacgccag ccgctacgcc gggtacgcca gcggctaccc
     1321 cggctacgcc cggtctcggc cctgcaccga tgccgccgcc agcacccgcg cccgccgagc
     1381 agagctccgg caacaatggc atgcgcattg gctcctacgt cggcttcggt gtcggcgcgg
     1441 tgggcctcgg tttgggaacg ctctttctct tgaaaggcgc gagcaagcgt tcggaggcga
     1501 acgacgcctt cgacgcctgc aaaagcgatc tgaactgcag agacctccct gcgacgagcc
     1561 cgaaaaaggc gaaggtcgat gacctagact ctcaggccac caaggctcaa cgcgtcggcg
     1621 tcgcgggcat ggttgtcggc ggccttggcg ttgccgcggg cgtcacgcta ctcgtcctga
     1681 gcggtaagag ctcgaccgag accaagacgg ggcgcctcgt tccgatcgtc agcccgaact
     1741 acatcggcgt ctcaggcgcg ttctgaggca ccggcttccc caccgggcac tcgccccggc
     1801 cgtaaccctg cagcgtgcga actgaccgag gttcggcgcc aagatacagc ttcactgcag
     1861 gtggcgcgcc gcccggcgtc agaagcggtt cgcacccact ctgctccgag gtggcgtgtt
     1921 gccttccaaa acttggggcg actgaccccg cttggtggga atcaagcaga cttttggcgt
     1981 acgctacgag agccctggag gactcttagg atggcgctcg agatcggtca gctcatcgaa
     2041 aacaagtatc gcatcactcg cttgattggt gagggtggga tgggcgccgt gtacgagggc
     2101 gaaaacattc gcatcaaccg gcgcgtcgcc atcaaggtgt tgcactcggc atttaccggc
     2161 aaccaagagg tgatgcaccg cttcgagcgc gaggcccaag cggcaggtcg catcggcaac
     2221 gaccacatcc tcgaggtgct cgatctcggc tctctcaacg acggtgacca ctacatcgtc
     2281 atggagttct tggacggtga gcctctcagc aaccgcatca agaaccgagg gcggctctca
     2341 ccgcaagagc tcacgcccat cttgcgccaa gtgctcgttg gcctcggcgc ggcacacgtc
     2401 gccggcatcg tgcatcgcga tctcaagccc gacaacatct tcatccttcg cgagaaggcg
     2461 ggcacgcgcg acttcgtgaa gatcatcgac ttcggcatct cgaagttcca accgctctcg
     2521 ggcgacggga tgaagatgac gcgtacgggt gcggtgatgg gcacgccgta ttacatgtcg
     2581 ccggagcaag cgagcggtag ccacgaggcc gatcagcgca gcgacattta ctcgatgggc
     2641 gtcatcatgt tcgaggcggt caccggcaag gtgcccttcg acgcggctac gttcaatcag
     2701 ctcatgttca agatcgtgct ggccgaagtg cccacggtcg agagcgtcgt gcccgacatc
     2761 gatcccgcgt actcgagcat catcgcaaag gcgatggcgc gcgacctgac tcatcgcttt
     2821 cagaacatcg aagagttcat tcgcgccctc gacgagtgga ccgcgcgcgg cacggcggtc
     2881 agcgttcccc cgcccaacaa cgccgaggac gctgggctcg ttccgcgagc gcatgggcgg
     2941 cccgcgatgg ggtctcacgc cgggctcgaa gccgggccgg tcggtgcgcg caccgccggc
     3001 aactgggcaa cgtctcagcc gggggccgaa gagattgcgg ggctgccgaa gaagggctcc
     3061 tcatccatca tcgcgatcgc cgcggctgcg gccatcgtcg tgttgggggg cggtgccttc
     3121 gccgcctaca gcgcgttggg gaaggggcag accgcggcgc agtcagcgcc gtccgacgcg
     3181 aagcccgcgg cggccgcgca agctcccgcc gcaccgaccg cgccgcctgc agcggtgacc
     3241 ccctcgccgg cagcgccgga ggtgaagccg gccgaggtga agcccgcaga ggtgaagccc
     3301 gccgaagcga agcctgctga ggtgaagccc tcgggggtga agccaacgcc gagcgtcgcc
     3361 gcggcccccg tggcagctcg tccggtagct gcagcgcccc ggccgcagct cccagcacag
     3421 cggccgcccg ctcgaccgaa gccagaaaaa tccaagccga gcgcttcacc cgacttcgga
     3481 tactgagccg gtcgcgcttt cgaaggtttc gcaaggcgtc gtttcgtttc gtcgcgcccg
     3541 cgccattcac cctatgggcg cgatgcgctt gtgctcgtgc tcgcgctcgc gctcgtgcgt
     3601 acgagtcggc gtgggacaac gacgcaggca gccgcgaacg cacgtgagcc gcccttttcg
     3661 tacgctcggc cccgcgttcc aaagctctgt cgtcggtggc gcttcgtcca cgtcggggtt
     3721 gggcgccttc gtcgccacgg aatcggcgac cgcgcctcgg cgccgtcgtc gcgacggaca
     3781 cggcgacacc gcagctaaat cataaagctc tttccaaatt cagaagagcc agaggcggga
     3841 atgagaacgg cgcgtgaact gcgtgcgtgc atctcccggc gaactcagcc gcctgggtgg
     3901 gtcgactcgg caccaggtct cgtcgcgaat cctcttcgcc tctggcagcg ctcgcaacat
     3961 cgaccgggac gcgccgcaca agccagctgg tttagccgcg atgggctcgg gcggagggcg
     4021 cgaggtgctc gcactcgctt cattttcggc ccgcgaagag cgagtgcaaa tcgtgcgtgg
     4081 agttgccgac gcgtcagata actccaccga ttcgtaatcc cgatctggga ccgggagtgc
     4141 aggagatcag aaacgaacaa gccccgattt cttccgcacc tttcgtatct gcgtgcgcct
     4201 agcccctcat tacgaaagcg tgatgcactc cgcgttttgt cgccggaata tctttgcgtc
     4261 gtcgcgtgtc cgttgcgagc tagcgccaaa caatgcgcgt tcggcggttc acgccgctgt
     4321 gctcaggtgt aacgcgctg
//
LOCUS       DASTKS010000064         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_9371,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000064 DASTKS010000000
VERSION     DASTKS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9371"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1209)
                     /locus_tag="VFQ35_01370"
     CDS             complement(58..1209)
                     /locus_tag="VFQ35_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929299.1"
                     /translation="MSLTSLQNGLLVLGLLEIATGCAGAVESSKFEDRARRTVPVPIC
                     LKPLERHGAEGVVSSLKPEDYWSLVLPSFDAGASTVDRSSPDCAGRPVFDNPELAQAE
                     GVRTGPLVVKLDDAVITPAADSLRIAWFRTHHFSDGTAAGPLALVRPREGYAEVYATG
                     FYRGRTAESHFSVERMGSRVLISASDEGCTGVKPNQSCQTLFSVFLMNAGYLNAAARF
                     PLDRVEYRSAPGVSGTAQYRLTATPVFQERAIRVQEQVIVRDSSQGVVRKSDLERIYT
                     LAGGQLAPSADSLWAQVAGDGSSPSMPSSIPSTTTTPGAARTPPSVPSAPRTPSVPSV
                     PHSPSVPSAPRAPSAPRLPSPPSPPQAPIPRAPSVPSPPSPPSGSLPKW"
     gene            complement(1212..1853)
                     /locus_tag="VFQ35_01375"
     CDS             complement(1212..1853)
                     /locus_tag="VFQ35_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929300.1"
                     /translation="MKSVVGFVVLVGLGSLGVGCSGAQDTATKNRTFYDWTVATGTSG
                     AVDFEQHYPPLDQAIAPPLPEYMGVTVVSGGVHLSRPKNWMLRDGVNTPGQSFVQYIS
                     PNAYSFAVYERPESTSELWRDVQQRFEDDAQSLGAKVVGKRVPMATLGSQGRAYTVER
                     QVEAAKAPFVSHSREYLLRGKTRIVLVQIVTDGEDLSAIDRELMRVVQTIEVL"
     gene            1947..>2159
                     /locus_tag="VFQ35_01380"
     CDS             1947..>2159
                     /locus_tag="VFQ35_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929301.1"
                     /translation="MLRVSCAAWFSLLLWGCGSDPGLRVTDGSDYGSYVRDRSGSAPS
                     TDLAAVRFKQGLCDGHDLRPETQLLTE"
BASE COUNT          371 a          681 c          751 g          356 t
ORIGIN      
        1 atcgcgcttg cggtttcgcg tagccgcgga tttctgcccg cgcttgcgct cgcgctctta
       61 ccacttaggc aagctgccgg agggcgggct tggcggggac gggaccgacg gagcgcgcgg
      121 gatgggcgcc tgaggcgggg aaggaggcga ggggaggcgc ggcgcagaag gcgcacgcgg
      181 ggccgatggg accgacggcg agtgcggcac ggagggcacc gagggcgtgc gtggcgcaga
      241 gggcaccgac gggggagtgc gggctgcgcc gggcgtcgtt gtcgtggagg ggatgctcga
      301 aggcatgctc ggcgagcttc cgtcgccggc aacctgagcc cacagcgagt ccgcgctcgg
      361 cgcgagttgt ccgcccgcga gcgtgtagat ccgctcgaga tcgctcttgc gaacgacgcc
      421 ttggctcgag tcgcgcacga tgacctgttc ctgcacgcga atcgctcgtt cttgaaagac
      481 gggcgtcgcg gtcagtcggt attgcgcggt gccggagaca ccgggcgcgg agcgatattc
      541 gacgcggtcg agcgggaagc gcgcggcggc gttcaggtag ccggcgttca tcaggaacac
      601 cgagaacagc gtctggcacg attggttggg cttgaccccc gtgcagccct cgtcgcttgc
      661 ggagatgagc acgcgtgagc ccattcgctc gaccgaaaag tgagactcgg cggtgcgccc
      721 ccgatagaag cccgtcgcgt agacctcggc gtaaccttcg cgagggcgaa ccaaggcgag
      781 gggacccgcc gccgtgccgt ctgaaaagtg gtgggtacga aaccacgcga tgcgcaatga
      841 atccgccgcg ggcgtgatca cggcgtcgtc gagctttacg accaatggac ccgtgcgaac
      901 accctcggct tgagcgagct ccgggttgtc gaagaccggg cgccctgcac agtctggcga
      961 tgagcgatcg acggtgctcg ctcctgcgtc gaagctcggc aacacgagag accagtagtc
     1021 ctcgggcttc agcgatgaaa cgacgccttc ggcgccatgg cgctcgagcg gcttcaaaca
     1081 aatcggcacc ggcacggtgc ggcgcgcgcg atcttcgaac ttggaggact cgacggcgcc
     1141 ggcgcagccg gtggcgatct cgaggagacc gagcaccaaa agcccgtttt ggagcgaggt
     1201 gagactcatt ttcacaggac ctcgatcgtt tgaacgacgc gcatgagttc gcggtcgatg
     1261 gccgaaagat cttcgccgtc cgtcacgatc tgaaccagaa cgatgcgggt cttgcctcgc
     1321 agcaggtact cgcggctatg actcacgaac ggcgccttcg ccgcttcgac ttgccgctcg
     1381 acggtatagg cgcgcccttg gctgccgagc gtcgccattg gtacgcgctt gccaactact
     1441 ttggcgccga gcgactgcgc gtcgtcctcg aaccgctgct gcacgtcgcg ccagagctcg
     1501 ctcgtggact ccggccgctc gtagactgcg aacgagtagg cgttcggtga aatgtactga
     1561 acgaacgatt gtccgggggt gttgacgccg tcgcgcaaca tccagttctt cggacgcgac
     1621 aggtggaccc ccccgctcac caccgtgacg cccatgtatt cgggcagcgg cggagcgatc
     1681 gcttgatcga gcggcggata gtgctgctcg aagtccacgg caccgctcgt gccggtggcg
     1741 actgtccaat cgtaaaatgt ccggttcttg gtggcggtgt cttgggcacc actgcaccca
     1801 acccccaacg agccgagccc cacgagaacc acgaacccaa cgaccgactt cacgacggcg
     1861 ggagactagc aaacccgggt tacgacccgc aagaacggcc gggcgagctt ctcttccgag
     1921 ggaacctttg ctagaaaccg gccgagatgc tgagggtgtc gtgcgctgcc tggttctcgt
     1981 tgctgctgtg gggctgtggg tcggatcccg gccttcgcgt cacggacggc tccgactacg
     2041 gatcttacgt acgcgaccgc tccggctcgg ccccgagcac cgacctcgcg gcggtccgct
     2101 tcaagcaggg cctgtgcgac gggcacgact tgcgcccgga gacgcagctc ctgaccgag
//
LOCUS       DASTKS010000065         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1792882, whole genome shotgun sequence.
ACCESSION   DASTKS010000065 DASTKS010000000
VERSION     DASTKS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792882"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(761..1990)
                     /locus_tag="VFQ35_01385"
     CDS             complement(761..1990)
                     /locus_tag="VFQ35_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9929302.1"
                     /translation="MELCSRERLASTLATLRPVLDIVEGRVEDVEPPSWCEQRGWSEF
                     LLSLSDADLQRCEAHGLDLCIAELDAAPASLRDLARAVTAAAEPPFFALGDARFEGDG
                     RQVSIRKRQQLAALLAAIDPMARRSARVVDVGAGRGALTRFCAVRFERTTLGIERSVE
                     RLLVATRNAPPGAALEFRTSDVLRDGLDLKPDDLAVGLHACGELGDALTASVAVARCH
                     LALIPCCLQKISAPFRAPLSRAAQGLAFSRSCLGLTNQTAQAEGVETSLERALDARMV
                     RYALSQLLRENGVEVATGEEMRGVNRRRAQRGLSALAARAFSLRGLAPPSAASVARHE
                     RSARARFDKIRRLALPRSMLSRLVELSIALDRASVLEERGLHTRVGTLFARSVSPRNL
                     AIFAGPDATSLPAYAAG"
     gene            complement(2036..>2439)
                     /locus_tag="VFQ35_01390"
     CDS             complement(2036..>2439)
                     /locus_tag="VFQ35_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HET9929303.1"
                     /translation="SIIPHSTGAARAIGKVLPELEGRLAGFSFRVPVPVVSVVDLTVQ
                     VQRVTDVAAVNAAFREAAASAALAGILGVSDEPLVSADFQGDARSSVVDAPLTMVSGG
                     HLVKVLAWYDNEWGFANRLIDTALHMASRAR"
BASE COUNT          433 a          822 c          779 g          405 t
ORIGIN      
        1 caccactggc gttcgacgaa ccgccactgc ccgtggagct accgcccgtc ccgaccgagg
       61 tcgagcctcc cgcgttgctc tttccgccgg agccggttgt gcccccggtg ccattgccgg
      121 aatccgatcc gctgcatccg acggccaacg ctgctgctgc cgcagcaaga acttgaagtg
      181 tgtaggtacg caacggttcc tccaatcggc gtattaccga tgtctgtatc gccttcgaga
      241 cggacggcct cgggggtgtt ttcgcgcaac cctctccagc gtgagggatt gcgtcgctcg
      301 aacttgtttc taaaccttgt ttcttaactt gtcgaccggc ttcagaagtg aatcactcgc
      361 gcggcaacct tgggcgttgc tcggcttctc agccttcggg cagtttcccc gcggggcgtg
      421 atccgttaca aaaagtatta gtgtcggcgt ggtcgagcct cattcgcggc gctgacacga
      481 acaaggcttc aacgcgccgc aacatctcat tgacccgcgt tgcgagcggt cgtgaaccga
      541 attcatgact gcaagaattt tcgaatatgt ataaactccg aagtctcgcg tagcgatccg
      601 gcggaccgtt cacgtgacgc gacgccgagc agcgacagcg tggcggcccc gggaccggat
      661 cggcgaggag cacgaccagc agcctctcga ctctgttcca caccgctcca cgcgcgagcg
      721 tgttcgaacc gcatttctcc ggccaaaggc ggagagccgt ctaccctgcc gcgtaggcgg
      781 gcaagctcgt cgcatcgggg cccgcgaata ttgcgaggtt gcgagggctg acagatcgcg
      841 caaacagagt tccgactcgc gtatgcaagc cgcgctcttc gagtacgctc gctctgtcca
      901 gcgcgatgct gagctcgacc aagcgcgaga gcatgctgcg cggcaacgcg agccgtcgaa
      961 tcttgtcgaa gcgggcccgc gccgagcgtt cgtgccgcgc gacgctggcg gcgctcggag
     1021 gggctaagcc gcgcagcgaa aacgcgcgtg cggctagagc cgaaaggcca cgctgggccc
     1081 ggcgccggtt gacgccgcgc atttcctcgc ccgtcgcgac ctctacgccg ttctcgcgca
     1141 aaagttgaga cagggcgtag cgcaccatgc gagcgtcgag tgctcgctcg aggctggtct
     1201 cgacgccctc ggcttgcgcg gtttgattgg tgagccccaa gcagctccgc gagaaagcga
     1261 gtccctgcgc cgcacgcgac agcggcgcac gaaagggcgc ggagatcttc tgcaaacagc
     1321 aagggatgag cgcgaggtgg caacgcgcta ccgcgaccga tgcggtcaac gcgtcgccga
     1381 gctcaccaca agcgtgcagg cctacggcta agtcgtcggg tttcagatcg agcccgtcgc
     1441 gcagtacgtc gctcgtccga aactcgaggg cggcgccggg cggtgcgttc cgcgtggcga
     1501 cgagcagacg ctcgacgctc ctttcgatgc cgagggtcgt tcgttcgaac cgtactgcgc
     1561 aaaatcgtgt cagcgccccg cgacccgcgc ccacgtcgac gacgcgcgcg ctgcgccgtg
     1621 ccatggggtc gatggccgcg agcagcgccg cgagctgttg ccgcttgcgg atcgacactt
     1681 gccgtccatc gccttcgaag cgcgcgtcgc cgagcgcgaa gaaaggtggc tcggccgcgg
     1741 ccgtgacggc tcgcgcgagg tccctcaaac tagccggcgc cgcgtcgagc tctgcaatgc
     1801 agaggtcgag cccgtgcgct tcgcagcgtt gcaagtcggc gtcgctgagc gatagcaaaa
     1861 actccgacca accgcgctgc tcacaccagc tcggcggttc gacgtcttcg acccgcccct
     1921 cgacgatatc gaggacgggg cgcaaagtag ccagcgtcga cgccaggcgc tcgcgtgaac
     1981 agagctccat ttccggccaa taccacgggc ggccgagcgc gtccgtcgag gcttttcagc
     2041 gcgcccgtga ggccatgtgc agcgcggtgt cgatgagtcg attggcgaag ccccactcgt
     2101 tgtcgtacca ggccagcact ttgacgaggt gcccgcccga taccatcgtc aacggagcgt
     2161 cgacgacgga cgatcgagcg tcgccctgaa agtcagcgga gacgagcggt tcgtcactga
     2221 cgcccaggat ccccgccagc gctgcactgg ccgcagcttc gcgaaacgcc gcgttcaccg
     2281 ccgccacgtc cgtcacgcgt tggacctgca ccgtgaggtc gaccaccgaa accaccggaa
     2341 ctggcacccg aaacgagaag cccgcgaggc gcccctcgag ctcgggcagc accttgccga
     2401 tggccctggc cgcaccggtc gaatgcggaa tgatcgaga
//
LOCUS       DASTKS010000066         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1792921, whole genome shotgun sequence.
ACCESSION   DASTKS010000066 DASTKS010000000
VERSION     DASTKS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792921"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..907
                     /locus_tag="VFQ35_01395"
     CDS             302..907
                     /locus_tag="VFQ35_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HET9929304.1"
                     /translation="MALPNRSLLTPLLMLLAVPLLVDCGGPRAVRGEEVAGLDDQAMS
                     TGLDKRDLDKLLHDNMQALQTSAVVRRWEGENRPQVSVVPLRNETSEHIDSSLDALIS
                     EIETTLVNAGHVRVISMMDQPKLVEEIKRQYSGAFDPTKVAAWGKQVGAKYIVTGKVF
                     SNDERQQGERRVQYFMFIQVLDVETGDILFQHKSGVTKAII"
     gene            924..2249
                     /locus_tag="VFQ35_01400"
     CDS             924..2249
                     /locus_tag="VFQ35_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929305.1"
                     /translation="MPSLLRVVCFALSLSACASHSDRTQAIRSALDAGDAKSALKYVN
                     ERLDVDNEKDVPNEAGSEKSLFLLDRAMILQQLDHYELSSRDLELADKQIDLLDLSRN
                     ALDSIGKYLFSDDVGRYKAPPYEKLLINTENMVNYLARGDLNGARIEARRLSVMQKYL
                     RDHEDPAQALTAAGSYLAGFTFEKSGKTSEALNYYDDALATGAYASLAAPVKRLLAQT
                     GSGSARLQKLAATTTEEIDETDAEILIVVGYGRVPFKIARRVPIGLALTYVSDALSPT
                     DRARANQLAAQGLVTWVNYPELGPAQGSIGIPRGRIDGKSAEFEPLATIDLEAKHAWD
                     EAKGAVVASAITRMLARVATGEVVRQGSGKDSLVGALLSLGVQATLTATDTPDTRSWE
                     ALPARLAVSRQRVKPGKHVIDVSVRGLKKQQEVELSSGGFAVVAHTVLF"
     gene            2349..>3116
                     /locus_tag="VFQ35_01405"
     CDS             2349..>3116
                     /locus_tag="VFQ35_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding EGF-like domain-containing
                     protein"
                     /protein_id="HET9929306.1"
                     /translation="MGSAHTKLGILALFSPLAVYACADGDFVDTEADGGSGGEFARGG
                     KSNGGTGGSVASGGVAHGGTGNEPNGGTGGATAGDAGNAGTGGDSGVSSGGTSSGGTS
                     SGGVSTGGAGGSGGDDLGDAGGFWGTPCALDCGRYGYCSDDGGGTRCVCLAGFAGERC
                     ELDVNECEPQNPCSGACTNTLGSFFCECPAGLAGIHCEHHLFQGMGVLPGHTNSHVSA
                     ISRDGKHLVGTSSSDSTRSAVSFDIASATLRAVNPNDA"
BASE COUNT          584 a         1015 c         1009 g          508 t
ORIGIN      
        1 cgcccaacgg ccgtcgagcc gagctagcca ctccgctctg aatcgggcgg aacttcgggc
       61 ggccacgcag ccaaagcgcg gcgcagagag atcgctgccg cgctttggtt ctgctcgtcc
      121 attgccggga aagacgccgt actttcaccc tgggtgtcga tgttttgctc gaaaacgtcg
      181 cccgcagcgg tttggcgcga ccccaccctg gcgagaaaat gatccgcggc ttaaatgatc
      241 ccgctgggtt gttccgtcaa accgagggat agctccgcac tggatcgaga aaggacttcc
      301 gatggctttg cctaatcgct cgttgctcac cccgctgcta atgctgctcg ctgtgccgct
      361 cctggtcgat tgcggcggcc cccgcgcggt tcgcggcgaa gaggtcgccg gcctcgacga
      421 ccaagccatg agcacggggc tcgacaagcg cgacctcgac aagctcctgc acgataacat
      481 gcaggcgctc caaacgtcgg cggtagtgcg gcgctgggag ggcgagaatc gcccccaggt
      541 ctcggtggta cctctgcgca acgaaactag cgagcacatc gacagctcgc tcgacgctct
      601 gatcagcgaa atcgaaacga cgctcgtgaa cgccggccat gtccgcgtca tcagcatgat
      661 ggaccagccc aagctcgtcg aagagatcaa gcgccagtac tccggtgcct tcgatccgac
      721 caaggtcgcg gcctggggca aacaagtcgg cgcaaagtac atcgtcaccg gcaaggtgtt
      781 cagcaacgac gaacggcagc agggcgagcg tcgcgtgcag tacttcatgt tcattcaggt
      841 gctcgacgtc gaaaccggcg acatcctgtt tcaacacaag agcggcgtga cgaaggccat
      901 catctgagct gcgggcgctg aacttgccgt cgttgctccg cgtcgtctgt ttcgcgcttt
      961 cgctttcggc gtgcgcgagc cattcggatc gcacccaagc aatccgaagc gcgctcgacg
     1021 ccggagacgc gaagagcgcg ctcaagtacg tcaacgagcg actcgacgtc gacaacgaga
     1081 aggacgtacc gaacgaggct ggcagcgaaa agtcgctgtt tttgctcgat cgggcgatga
     1141 ttttgcagca gctcgatcac tacgagctct cgagccgcga cttggagctc gccgacaagc
     1201 agatcgatct cttggatttg tcgcgcaacg cactcgacag catcggcaag tacctgtttt
     1261 cggacgacgt ggggcgctac aaagcgccgc cctacgagaa gctcctcatc aacaccgaga
     1321 acatggtgaa ctatctcgcg cgcggtgatt tgaacggcgc gcgcatcgag gcgcggcggc
     1381 tctcggtgat gcagaagtac ctgcgcgacc acgaagaccc ggcccaagct ctaaccgcgg
     1441 ccggcagtta cctcgccggc ttcactttcg agaagagcgg caaaaccagc gaggcgctca
     1501 actactacga cgacgcgctc gcgaccggtg cgtatgcttc gctcgcggcg ccggtaaagc
     1561 gcctcctcgc gcaaacgggc tccggttccg cgcgcctcca gaagctcgct gcgaccacta
     1621 cggaggagat cgacgagacc gacgcggaga tcctcatcgt ggtcggctac gggcgagtcc
     1681 ccttcaaaat cgcgcgacgc gtaccaatcg gcctcgcact cacgtacgtc tcggacgcgc
     1741 tgagcccgac cgaccgcgcc cgcgcgaacc agctcgcagc tcaagggctc gtcacgtggg
     1801 tgaactaccc agagctcggc cctgcccagg gcagcatcgg cattccgcgc ggccgcatcg
     1861 acggcaaatc cgccgagttc gagccgctcg cgacgatcga tctggaggcc aagcacgcct
     1921 gggacgaagc caaaggtgcc gtggtggcgt ccgcgatcac gcgcatgttg gcgcgcgtcg
     1981 ccaccggcga agtcgtgcgc caaggcagcg gaaaagactc cctcgtcggc gcgctgctca
     2041 gcctcggcgt gcaggcgacg ctcaccgcaa ccgacacccc cgacacccgt agctgggagg
     2101 ctttgccggc gcgactcgcc gtgagccgac agcgtgtcaa accgggtaaa cacgtgatcg
     2161 atgtctcggt tcgtggcctg aaaaagcagc aagaagtcga gctctcgtcc ggcggcttcg
     2221 cggtcgtagc ccacaccgtg ctcttctaat agctcgttcg cgagcgccac ccctggcacg
     2281 cagtggcgaa gcgcgacggc gtgatcgtcg tcattgcgaa cgcggccgcg aggcaaagac
     2341 ttccgtccat ggggtctgcg cacacgaagc tcgggatctt ggcgctgttc tcgccgctgg
     2401 ctgtctacgc ttgcgccgac ggcgacttcg tcgacacgga agccgacggg ggctcagggg
     2461 gtgagttcgc tcgcggcggc aagagcaatg gaggaacggg gggctcggtc gcttccggag
     2521 gagtcgctca cggcggcacc ggcaacgagc cgaacggcgg cacgggcgga gcgaccgccg
     2581 gtgacgcggg caacgcgggc acgggcggag acagcggcgt ttcgagcggc gggacttcga
     2641 gcggcgggac ttcgagcggc ggcgtttcaa ccggcggcgc cggcggatcg ggcggggacg
     2701 acctgggcga cgcgggcggt ttctggggaa ctccgtgtgc gctcgattgc ggccgctacg
     2761 gttactgcag cgacgatggc gggggaacgc gctgcgtgtg cctcgccggc ttcgctggcg
     2821 agcgctgcga gctcgatgtc aacgaatgcg agccgcaaaa tccttgttcg ggcgcgtgca
     2881 cgaacacgct cggcagcttc ttttgcgagt gccccgctgg gctcgcgggc atccactgcg
     2941 agcaccactt gttccagggc atgggcgtac tcccgggtca tacgaatagc cacgtctccg
     3001 cgatcagccg cgacggcaaa cacctggtgg gcacttcctc gagtgattcg acccgcagcg
     3061 cagtgtcgtt cgacatcgcg agcgccacct tgcgagccgt taatccaaac gacgcg
//
LOCUS       DASTKS010000067         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_10004,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000067 DASTKS010000000
VERSION     DASTKS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10004"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..632)
                     /locus_tag="VFQ35_01410"
     CDS             complement(132..632)
                     /locus_tag="VFQ35_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9929307.1"
                     /translation="MSDSSLFAPVCSITATRRRRFLWAAWWTRRPERSPFVKPDASEG
                     GARTREEALASAEKAAGCRLTLIDGSWARAWARVLVGDEPFSGRRDEAVPKPANHDKA
                     PESPHFALLGLTPRASDEEVKRAYRSRALETHPDRGGSAEAFRAVHAAFEHISKRRAR
                     APKKRR"
     gene            complement(724..1749)
                     /gene="hypE"
                     /locus_tag="VFQ35_01415"
     CDS             complement(724..1749)
                     /gene="hypE"
                     /locus_tag="VFQ35_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET9929308.1"
                     /translation="MTGILDPFNGRVRLAHGAGGRAMRDLILSVFLEGVSDPEARALG
                     DAAVLPLGDRFLVVTTDAHVVKPAFFPGGDIGRLAICGVVNDLAMLGCTEVAGVTSSV
                     IIEEGFPIESLQRIHRSMLKACEEGGTRVITGDTKVMQRGEIDGVVLSSSAFGVAECI
                     VRDSGLEPGDAIIVTGTIGDHGLAVLSARHELGLEGELTSDVAPINDLVRSVLRVAGP
                     ALHAMKDPTRGGVTSALVEMAEKAKVSIVLDERAVPISPAARAASELLGIDPLCVANE
                     GKALLGVQPGAVDDVLAALRAHPLGLHAARIGAVLEGERGAVVLDTGFGKRRLIERDD
                     TPLPRIC"
BASE COUNT          455 a          879 c          768 g          412 t
ORIGIN      
        1 gtttcttggc tctcgcgacg ggcaggcaac ttgggacacc ccctagtgca cgcttcggcg
       61 gccaagggca gcgccccgaa tcggagcaat ctcaccagcg ctacaccgcc aagcgcgtca
      121 gaagcgcgga atcagcgccg cttcttcggg gcccgtgcgc gtcgtttgct gatgtgctcg
      181 aaggccgcgt gcaccgcgcg aaacgcctcg gcagaaccgc cccggtctgg gtgcgtctcg
      241 agcgctctcg agcggtacgc gcgcttgacc tcctcgtcgc tcgcgcgtgg agttagcccg
      301 agcagggcaa agtgcgggga ttctggagcc ttgtcatgat tggctggctt tgggactgct
      361 tcgtctcgcc gcccactgaa gggctcgtca ccgaccaaca cccgcgccca ggctcgagcc
      421 cacgacccgt cgatgagcgt gagccgacaa cccgccgcct tctcggcgct cgcgagggcc
      481 tcttctcgcg ttcgcgctcc gccttccgaa gcgtccggct tcacgaaggg ggagcgctcg
      541 ggtcgtcgtg tccaccaagc tgcccacaag aagcgccgac gccgcgtcgc ggtgatcgag
      601 caaaccgggg cgaacagcga cgaatcggac atacgcccgc tcgccatagc gcgaggcgga
      661 tcccagggca aagggcgcct tctctcggca ccctcgggag tgcgaacgga tgcatgcgcg
      721 ccgtcagcag atccgcggta gcggtgtgtc gtcgcgctcg atgaggcggc gcttgccaaa
      781 gccggtgtcc agcacgacgg cgccgcgctc accttccagt acggctccga tgcgcgcggc
      841 gtggaggccg agcggatgag cccgcaacgc ggccaatacg tcgtctacgg cgcccggctg
      901 caccccgagc aacgccttgc cttcgttcgc gacgcagagc ggatcgatgc cgaggagctc
      961 cgaggcagcg cgtgcggcag ggctgatcgg caccgcgcgt tcgtcgagga ctatcgagac
     1021 cttcgccttt tcagccatct cgacgagcgc gctcgtgacc ccaccgcgcg tcggatcctt
     1081 catggcgtgg agcgccgggc ccgcgacccg aagcaccgag cgaacgaggt cgttgatggg
     1141 cgcgacgtcg gacgtgagct ccccctcgag cccgagctcg tgacgcgccg agagcactgc
     1201 gagcccgtgg tcaccgatcg tcccggttac gatgatggca tctcccggtt ccagccccga
     1261 gtcgcgcacg atgcactcgg cgacgccgaa tgcgctcgac gacaacacga cgccgtcgat
     1321 ctcaccgcgc tgcatgacct tggtgtcgcc ggtgatcacg cgggtgcccc cttcttcgca
     1381 cgccttcaac atggaacggt ggatgcgctg aagcgactcg atggggaacc cctcttcgat
     1441 gatgacactc gacgtaaccc ccgcgacctc ggtgcacccg agcatcgcga ggtcgttcac
     1501 gacgccgcaa atcgcgagtc gcccgatgtc accgccggga aaaaacgctg gttttacgac
     1561 gtgagcatcg gtggtcacga cgagaaagcg atcgcccagc ggcagcacgg cggcgtcgcc
     1621 cagcgcccgc gcctccggat cgctcacccc ctcgagaaaa accgaaagga tcaagtcgcg
     1681 catcgcgcgc ccgccggcgc catgggcgag ccgcacgcgt ccgttgaaag gatccagaat
     1741 gccagtcact tcgacgcctc gctcacaccg tgctcgtacc agctacggca cacaccctcg
     1801 ctgctcactg cacgctccac tggaacctcc ggacggcacg ctgcgccgaa caacttacaa
     1861 tcccgcggcg tcgctcggcc agtcatgatt gctccgcagc gacaatcgtc gcgagacacc
     1921 gagatcgcgg cgtgcccggc cgcgaaatat ccttcgactt cgctgtccgg actctctgct
     1981 acgacttcca tcgcggcggg gacccacttg tgcgcggaaa gcacgaagcg tgcagaatag
     2041 gaaggacgcg caagggcgcg cgcccgttct tcgcgagaat cgcctcccag tcgagcttcc
     2101 aagggcccgc gtcgcgcgcc gcttctccgc cttcaccgtt cggtttcaga agcatacgtt
     2161 tcacttgtta ccccacctga agcgattcca gaatgaactc gatcagtgcg actcgcgttt
     2221 ctgccagtct tcgttatggc gcggtaagaa aacctaaccc acacacgcag agcagctgga
     2281 tttcgtattc gccggcgatt ccccaatgcc gatcagcaac tagccggcga gttcgagacc
     2341 ggcgaatatc ggcatgaccc cgtcgtcaaa aagcaagtct gggcgatgtt ccacggcaat
     2401 cacgagcgac tcggctccat cgagctgccg agtacggggt agcggtgcgg acgcgctatg
     2461 gaaaatcgtt actctaccga gtcaccctcg acgattatct tcagcggcga accc
//
LOCUS       DASTKS010000068         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_456051, whole genome shotgun sequence.
ACCESSION   DASTKS010000068 DASTKS010000000
VERSION     DASTKS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456051"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFQ35_01420"
     CDS             complement(<1..854)
                     /locus_tag="VFQ35_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9929309.1"
                     /translation="MARRALAGRVRVGISGWSYKPWRGVFYPAKLRIADQLSFASRAF
                     STIELNGTFYSLQLPSSYRAWYEATPEDFVFAVKGGKYITHLKRLREPEQGLANFFAS
                     GVLELKDKLGPILWQLPPTLQFEPERLKQFFEFLPRTHADAAKLARRHSPRLSSRVAF
                     GTEGRARIRHALEVRHPSFVCPEAVELMRRFGVAPCIADSAGLFPRLEDLCADFVYVR
                     LHGAEELYTSGYSNAELANWASRIRAWRQGRDARAPYKGDPGARGDGKARDVFVYFDN
                     DVKVRAPF"
     gene            complement(915..1772)
                     /locus_tag="VFQ35_01425"
     CDS             complement(915..1772)
                     /locus_tag="VFQ35_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929310.1"
                     /translation="MRNSLVDRSSTSDEDSWFDEDARVALREAEARWGTAFGRASRKE
                     LARAATAALQGQSRGGATPQATLAGIEQCHVARHGKLALRAELEDEVERDAARRSDAA
                     LVTYEAKWSARLERMQRTQPGRWSVREFSAEELRAELTLSLIELVIARDDEFERYERA
                     GREFGFSFLLSARAALRRKYRLRVELTEPTVLADPVPTLEERLIAEQTRAVLGLAGAA
                     AERALSRPQRRWLSALKLSANAGAFFESSGRVNLAAASRLLQKNRSSAKRAFAELQRQ
                     FQAELEKLV"
     gene            1884..>2400
                     /locus_tag="VFQ35_01430"
     CDS             1884..>2400
                     /locus_tag="VFQ35_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929311.1"
                     /translation="MSELRADCARCEGLCCVVLAFDQSPAFAFTKPLNTPCPHLSASN
                     RCEIYPTRGEAGFHGCGHFDCYGAGQRATELVPGPSWREDPERARRLFAAFCVALELH
                     ELLLLLDATSHLALPTAHASERDALVEGLERDARRVATEAVDISQRRRRVHEFLRELA
                     PFVAPMHRRRLR"
BASE COUNT          416 a          802 c          763 g          419 t
ORIGIN      
        1 tcgaacggcg cccgtacttt cacgtcgttg tcgaaataca cgaatacatc gcgcgccttg
       61 ccgtccccgc gcgcgcctgg atcgcccttg taaggcgcgc gcgcgtcgcg cccttgtcgc
      121 cacgcgcgga tcctactggc ccaattcgcg agttcggcgt tgctgtatcc gctcgtgtaa
      181 agctcctcgg caccgtgcag ccgtacgtac acgaagtcgg cgcacaaatc ttccaggcgg
      241 gggaacagcc cggcggagtc ggcgatgcaa ggagcaaccc cgaagcggcg catgagctcg
      301 accgcctcag ggcagacgaa gctcgggtga cgaacttcga gcgcgtgacg tattctcgcg
      361 cgtccttccg tcccaaaggc gactcgcgac gaaagccgtg gtgagtgccg gcgcgcaagc
      421 tttgccgcgt cggcatgggt gcgcggcaag aactcgaaaa attgtttcag tcgctccggc
      481 tcgaactgaa gtgtcggggg cagctgccac aggatggggc cgagcttgtc cttgagctcc
      541 agcacccccg acgcgaagaa gtttgcgagc ccctgctctg gctcgcgaag ccgcttcaaa
      601 tgcgtgatgt actttccgcc cttgaccgca aagacgaagt cttcgggagt cgcttcgtac
      661 caggcgcgat aactcgacgg gagctgcagc gagtaaaacg ttccattcag ctcgatcgtc
      721 gagaacgcgc ggctcgcaaa gctcaactgg tccgcgatgc gtagtttggc ggggtaaaat
      781 acgccccgcc aaggcttgta gctccagcca gaaatcccga cgcgaacgcg accggcgagg
      841 gctctccggg ccatggctgc caagttgcaa gccgtgttcc agaaaacgct cgtacgtctg
      901 tcgcccaccg gacgctagac gagtttctcg agctcggcct ggaactggcg ctgcagctcg
      961 gcaaacgctc gcttggccga agagcgattc ttctggagta ggcgtgacgc tgccgcgagg
     1021 ttgactcggc cgctcgactc gaaaaacgca ccggcgttgg cactgagctt caacgcggag
     1081 agccagcgac gttggggccg cgacaacgcc cgctcggccg cggcgccggc gagaccgagc
     1141 accgcgcgcg tttgctctgc aatcagccgt tcctcgagcg tcggcacggg gtcggccaat
     1201 accgtcggct ccgtgagctc gactcggagc cggtacttgc ggcgcagggc cgcgcgcgcc
     1261 gagagcaaga acgaaaaccc aaactctcga ccggctcgct cgtagcgctc gaactcgtcg
     1321 tcgcgagcaa tgaccaattc gatgagcgat agcgtgagct cggcgcgcag ttcttccgcc
     1381 gaaaactcgc gcaccgacca acgaccgggc tgcgtacgct gcattcgctc gagccgtgcc
     1441 gaccacttcg cctcgtaggt aacgagagcg gcatccgagc gtcgcgcagc gtcgcgctcc
     1501 acttcgtcct ccagctccgc ccggagcgcg agcttcccgt gccgagcgac gtgacactgc
     1561 tcgatgcccg cgagtgtcgc ttgcggtgtt gcccctccgc gcgattggcc ctggagcgct
     1621 gcggttgcgg cacgagcgag ctctttgcgc gaagccctcc cgaacgccgt gccccagcgc
     1681 gcttccgctt cacggagcgc gacgcgagcg tcttcgtcga accacgagtc ttcgtcgctc
     1741 gtcgaggatc tatcgaccag tgagtttctc acgaaggtgt ccccttctcg tgactcaaat
     1801 gttgcgcatt ttgtcggacg gcgctgcatc ttctcgaaaa tgcgcaactt cgcgggctgc
     1861 gccgagctca cgaagcagcg aacatgagcg agctccgagc cgactgcgcc cgctgcgaag
     1921 gattgtgctg tgtcgtgctc gcgttcgacc aatcgccagc gttcgccttc acgaagccgc
     1981 tgaacacgcc ctgccctcac ctgtcggcgt caaatcggtg cgagatctac cctacccgag
     2041 gggaggccgg ctttcacggg tgcggtcact tcgactgtta cggagctgga cagcgggcga
     2101 cggagctcgt cccggggccg agctggcgag aagaccccga gcgcgcccgc cgattgtttg
     2161 cggctttctg tgtcgcgctc gagctccacg agctcctgct gctgctcgac gcgacgagtc
     2221 acctggcgct gcccacggcg cacgctagcg agcgcgatgc gctggtcgaa gggctcgagc
     2281 gcgacgcccg gagggtggcg acggaggccg tcgatatcag ccagcgccgc cgcagggtgc
     2341 acgaattctt gagagagttg gcaccgttcg tcgcgcccat gcaccgtcgc aggctccgcg
//
LOCUS       DASTKS010000069         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_232209, whole genome shotgun sequence.
ACCESSION   DASTKS010000069 DASTKS010000000
VERSION     DASTKS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232209"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFQ35_01435"
     CDS             complement(<1..970)
                     /locus_tag="VFQ35_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9929312.1"
                     /translation="MRWPLALLSSFALLGLGRFASAEPAVHTVASGQRLGSIAKRYGV
                     SIESICNANGFTRRDKLHPGQRLLIPDPSDKDGSRAAEEQNVAPAVAQETPKPSGALQ
                     ELELEGHQRAFYYEPTGPGRLGMRPVLVYLHGRGGRPEEDCRRWASVARNLGWLVCPA
                     GPSPHGNGRAWDNNWTSAHQITMAAIQGLRARYGRRVQLWGNTLVGFSEGAYAAMNVG
                     VREPRTFNRWLILAANTRYWGGPGLEALETARERVRRVYLITGEHDEVVDGTRQVEVW
                     LRKARVTTRISTPKDMGHELALDRKPFLYKQALRWLNGLDKKAVEAS"
     gene            complement(1048..1722)
                     /locus_tag="VFQ35_01440"
     CDS             complement(1048..1722)
                     /locus_tag="VFQ35_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929313.1"
                     /translation="MSGQKPRAIVSWSSGKDSAFSLHVVREQAELELVALFTTMNHVH
                     DRVAMHAVRRELVRAQAESAGLPLWEVEIPSPCSNEEYENAMRGLVARARDLDVEVVV
                     FGDLFLEDIRRYREERLQGTGLRPSFPLWGIETSTLATRMFDAGVSAIVTCIDPKKLS
                     HACAGKRWDPAFVAELPEGTDPCGENGEFHTFVTHGPMLRRPLEVVVGQTVERDGFVF
                     ADVLSG"
     gene            complement(1719..2246)
                     /locus_tag="VFQ35_01445"
     CDS             complement(1719..2246)
                     /locus_tag="VFQ35_01445"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HET9929314.1"
                     /translation="MRKILGIAALALAAASGCRSESGAGARVEKAVSTPSASSAAAKP
                     IQRFGDAISPVLEPTAITQVLAAPEKFQGRPVVVEGYVRRACSRRGCWMELSEGADPN
                     LEGCRVTFKDYGFFVPTDSAGSSARVAAAIEVASLTPATVEHMEREGAKITHKSGDGS
                     AREVRLVASGVELWR"
     gene            complement(2272..>3372)
                     /locus_tag="VFQ35_01450"
     CDS             complement(2272..>3372)
                     /locus_tag="VFQ35_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929315.1"
                     /translation="VAKVEKTKLTVTVYQSSDARVLITLQFKAAPGPKLESIIQKRLV
                     QKLYGAFGIESAEKSDEAEEEEAGEEESGGEEATEPADDAPASEEPAESSASESEAPA
                     PTGAGFAPKPLILRAGVHLSQRSFAFKDTLSQLTPGRPVTHPLRDYNGGTDLAVFIRG
                     EIYPGAFLKAEGFGSNLGIVGSFSYGIPSSTQYKPAGGAAQTLKTDVNEFTVGLRGRF
                     PFSAKAVLGFSGVYGQQRYFLKNDEKSALVPDIQYSYLRLGPDLLLEFGKFSVEAYVA
                     ARLVTGTGELENKSLWFANVGARGLDAGLTLGFAVTPSISVLGGADFTRYGFNFNPIP
                     DDSLYVAGGATDQYVGGWLGIGFHVPGTPASE"
BASE COUNT          548 a         1095 c         1127 g          602 t
ORIGIN      
        1 cgctcgcttc gacggccttc ttgtcgaggc cgttcagcca gcgtagcgct tgcttgtaga
       61 ggaacggctt gcgatcgagc gcgagctcgt ggcccatgtc cttgggcgtc gagatgcgcg
      121 tcgtgactcg cgctttgcga agccagactt cgacctgacg cgtcccgtcg acgacctcgt
      181 cgtgctcgcc ggtgatgaga tagacgcgcc gcacgcgttc acgcgccgtt tcgagcgcct
      241 cgaggccggg accgccccag tagcgcgtgt tggccgccaa gatcagccaa cggttgaagg
      301 tgcgcggctc gcgcacgccc acgttcatcg cggcgtaagc accctcgctg aagcccacga
      361 gcgtgttccc ccagagctgt acgcgtcgcc cgtagcgcgc gcgcaggccc tggatcgccg
      421 ccatcgtgat ctgatgggcg ctcgtccagt tgttgtccca agcgcggcca ttgccgtgcg
      481 gggacgggcc agcggggcac acgagccaac cgaggtttcg agcgacactg gcccagcgcc
      541 ggcaatcctc ttccgggcgg ccgccgcgac cgtggagata gacgagcact gggcgcatgc
      601 cgagccggcc gggaccggtg ggctcgtagt agaaagcgcg ctggtgcccc tcgagctcga
      661 gctcctgtaa tgcgccgctc ggcttcggtg tctcttgagc aaccgcgggc gctacgttct
      721 gctcttcggc ggcgcgcgat ccatccttgt ccgacggatc cgggatcaag agcctttgtc
      781 ccgggtgcag cttgtcgcgg cgggtgaagc cgttcgcgtt gcagatcgat tcgatcgaga
      841 cgccgtatcg tttcgcgata ctaccgagcc tctgcccgga ggccacggtg tggactgccg
      901 gttcggccga agcgaagcga ccaaggccta gtagtgcgaa agagctgagc agggcgagcg
      961 gccaccgcat gggtctagtc ggctagcata accgagcccc ggcggggaat tccccgagca
     1021 tccgcggttt cggatcgacg aatttggtca gccggacaag acgtcggcaa agacgaaccc
     1081 atcccgctcg accgtctggc cgacgaccac ctcgagcggc cttcggagca tcggcccgtg
     1141 ggtgacgaag gtgtggaatt cgccgttttc gccgcacgga tccgttcctt cgggcagttc
     1201 cgcgacgaaa gccgggtccc agcgctttcc ggcgcaggcg tgcgacagct ttttcgggtc
     1261 gatgcacgtc acgatcgccg agacaccggc gtcgaacatc cgcgttgcga gcgtgctggt
     1321 ctcgattccc cacagtggga acgaagggcg gagaccggtg ccctgcaggc gctcttctcg
     1381 gtagcgccgg atatcctcga gaaaaagatc gccgaagacg accacctcga cgtcgaggtc
     1441 acgcgctcgc gcgaccaggc cgcgcatcgc gttctcgtac tcttcgttcg agcaagggct
     1501 cgggatttcg acttcccaga gcggtagccc cgcgctttcg gcctgagcgc ggacgagctc
     1561 acgacggacg gcgtgcatcg cgacgcggtc gtgaacatgg ttcatggtcg tgaagagcgc
     1621 gacgagctcg agctctgctt gctcacgcac gacgtggagc gaaaacgcgc tgtctttccc
     1681 gctgctccaa gagacgatgg cgcggggttt ctggccgctc aacgccaaag ctcgactccg
     1741 ctcgcgacca accggacctc gcgggcgctc ccgtctccgg acttgtgcgt gatcttcgcg
     1801 ccttcgcgct ccatgtgctc gaccgtcgcc ggggtgaggc tcgcgacctc gatcgccgcc
     1861 gcgacccgcg ccgaagagcc tgcggagtcc gtgggcacga agaagccgta atctttgaag
     1921 gtgacgcggc agccttcgag gttcggatcg gcgccctccg agagctccat ccaacatccg
     1981 cgccgtgagc acgctcgccg gacgtagcct tcgaccacca ccggccgccc ctgaaacttc
     2041 tcgggcgcgg cgaggacctg cgtgatggcg gttggctcga gcaccgggct tattgcatca
     2101 ccaaagcgct gtatcggctt ggccgcggcc gatgaagcgc tgggagtcga aaccgccttt
     2161 tccacgcgcg cacccgcgcc cgattcgctc ctgcaacccg acgctgccgc gagcgccaat
     2221 gcagcgatac ccaagatctt ccgcatgcgg tcgagtatag ctgcggaacg gctactcgct
     2281 cgcgggggtc ccgggcacgt ggaaaccgat gccgagccag ccgccgacgt actggtcggt
     2341 agcgccgccc gcgacgtaca gtgaatcgtc gggaatggga ttgaagttga agccgtagcg
     2401 cgtgaaatcg gcgccgccga gcacgctgat gctcggcgtg acggcgaagc cgagcgtgag
     2461 tccggcatcg aggccgcgtg cgccgacatt ggcgaaccac aggctcttgt tttcgagctc
     2521 tccggttccg gtcacgagcc gggccgcgac gtaggcttcg acagagaact tgccgaactc
     2581 gagcaagaga tccgggccga ggcgaaggta ggagtactgg atgtccggca ccaacgcgct
     2641 tttttcgtcg ttcttcagga agtagcgctg ctggccatag acgccgctga acccgagcac
     2701 ggccttggcg ctgaatggga agcgcccgcg caggccgacg gtgaactcgt tcacgtcggt
     2761 cttcagcgtc tgcgccgctc ctcccgcagg cttgtattgc gtgctcgagg gaatgccata
     2821 ggagaagctg ccgacgattc cgagattcga gccaaagcct tcggctttga ggaatgcgcc
     2881 tgggtaaatc tcgccgcgga tgaacacggc gaggtcggtg ccgccgttgt agtcacgaag
     2941 agggtgagtg acggggcggc cgggcgtgag ctgcgagagg gtgtccttga aggcgaacga
     3001 acgctgcgag aggtgaacgc ccgcgcgcaa aatgagcggc ttgggggcga acccggcgcc
     3061 ggtcggagca ggcgcttcgc tctcgctggc cgagctttcg gcgggctcct cgctcgccgg
     3121 ggcgtcgtcg gccggctcgg tcgcttcctc gccgccgctc tcttcttcgc ccgcttcctc
     3181 ttcttcggcc tcgtccgact tctccgcgga ctcgatgccg aacgcgccgt agagcttctg
     3241 cacgaggcgc ttttgaatga tgctctcgag cttcggtccg ggggcagcct tgaactgcag
     3301 cgtgatgagc acgcgagcgt ccgaggattg gtagacggtg acggtgagct tcgtcttttc
     3361 gaccttggcg ac
//
LOCUS       DASTKS010000070         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_232503, whole genome shotgun sequence.
ACCESSION   DASTKS010000070 DASTKS010000000
VERSION     DASTKS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232503"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFQ35_01455"
     CDS             <1..936
                     /locus_tag="VFQ35_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HET9929316.1"
                     /translation="SGSTSSLKADPWDPRAGIGPADALHADFTVATDGSGSHSTIQAA
                     INASSGSTPRSILIKPGTYRETVSVPSSAPPITLFGLDRDASRVVIVSGKSSGDAGGT
                     AQSATLTAKSAGFRLLNLTVSNDFATPASGSNIQAVALYTTGDKTVLENVRLHGFQDT
                     LYVDSPSPTGIARVYVKSSFIEGDTDFIFGRATAVFEDTTIHYLSSRKGTGAGVHFAP
                     STHVDNPYGFLAIGCTFDADGASPSNKISLGRSWDQSSTTPTPNGQLVIRESVLGAHI
                     RKADPWTAAATSGRAYSASGNRFSEYCNSGPGAGG"
     gene            complement(1027..1623)
                     /locus_tag="VFQ35_01460"
     CDS             complement(1027..1623)
                     /locus_tag="VFQ35_01460"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HET9929317.1"
                     /translation="MTELPLRCACGKLKGSVSLSADSGVHVVCYCKDCQAFARFLGRG
                     DITDQWGGTDIFQMAPSAVRISEGADAIRCVRLSAKGMHRWYCGECKTPIGNTLGPKL
                     PFVGLISSIVDYASAGATPDSVLGKPVAYGFPEAALGGPPPHTKHNPKLKFLGKVSAK
                     LAKWWLTNAGWPSPFFDETTRAPRAVVQVLSPEERARL"
     gene            complement(1728..2096)
                     /locus_tag="VFQ35_01465"
     CDS             complement(1728..2096)
                     /locus_tag="VFQ35_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9929318.1"
                     /translation="MQFLIVIKLENNTDYEAGKPLPEGFDAKMGLLMGEWAKQGVMLS
                     AAGLKPTSRGSRVRLSSGKVSVTDGPFTEAKEVIGGFFMIEAKDKAAALELTRAFVDL
                     HRGSFPENFLLECELREVNH"
     gene            complement(2239..>2442)
                     /locus_tag="VFQ35_01470"
     CDS             complement(2239..>2442)
                     /locus_tag="VFQ35_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929319.1"
                     /translation="ASCASGSPSGSPAMTPRLIFADEETGLGVGFTMFQNADTDMHLF
                     KMYGGKVYGVSAILGAAGSSGWD"
BASE COUNT          448 a          819 c          717 g          458 t
ORIGIN      
        1 agcggctcga cgagctcact caaggccgat ccgtgggacc cccgcgcggg catcggtcct
       61 gccgacgctc tacacgccga cttcacggtc gcaaccgacg gcagcggttc tcactccacg
      121 atccaagctg cgatcaacgc gagttctggt tctactcctc gctccatatt gatcaaaccc
      181 ggtacgtatc gtgaaaccgt ttcggtaccg agctctgcac cgccgatcac tctgttcgga
      241 ctcgatcgtg acgcgagccg cgtcgtcatc gtcagcggca agtcgtccgg agacgccggt
      301 ggtactgcgc agagcgcgac cctcaccgcg aagtcagcgg gctttcggtt gctgaacctc
      361 actgtctcga acgactttgc gacaccggcg agcggttcga acattcaggc ggtcgcgctg
      421 tacaccaccg gtgacaagac cgtgctcgag aacgttcgct tgcacggctt ccaagacacg
      481 ctctacgtcg actcgccttc gccgaccggt atcgcgcgcg tctacgtcaa gagcagcttc
      541 atcgagggcg acaccgactt catcttcggg cgcgccactg ccgtcttcga ggacactacc
      601 attcactacc tgtcgtcgcg caaagggacg ggcgcgggcg tccacttcgc gccgagcact
      661 cacgtcgata acccatacgg tttcctcgcg attgggtgca ctttcgacgc ggacggcgcg
      721 agccccagca acaagatctc actcggtcgc tcgtgggacc aaagcagcac gactccgacg
      781 cccaacgggc agctcgtgat ccgggaatca gtgctcggcg ctcacattcg caaggccgac
      841 ccctggaccg cagcagccac cagcggacgc gcctacagcg cgagcggcaa tcgcttctcc
      901 gaatactgca acagcggtcc cggcgccggc gggtgaaccg gaatcgctcg cgtgacacgc
      961 tcgatcgcac cggggtacgc gatgcactcg aacgcacccg cgttgcgcga gcccgttcgc
     1021 gggcccttac agccttgctc gctcctccgg tgagagaact tgcaccaccg cgcgcggcgc
     1081 ccgcgttgtc tcatcgaaga atggcgacgg ccagcccgca ttggtgagcc accacttggc
     1141 aagcttagcg ctcacctttc cgaggaactt cagcttgggg ttgtgcttcg tatgaggcgg
     1201 aggaccaccg agcgccgcct ctggaaagcc ataggcgaca ggctttccga gcacgctatc
     1261 tggcgtcgcg cctgcgctcg cgtagtcgac gatcgagctg atcaagccca cgaagggaag
     1321 cttcgggccg agcgtattgc cgatcggcgt cttgcattcg ccgcaatacc agcggtgcat
     1381 cccctttgca gaaagacgca cgcagcggat cgcgtccgcc ccttccgaga tgcgcaccgc
     1441 cgatggcgcc atctgaaaga tgtccgtgcc gccccattgg tcggtgatgt cgccgcgccc
     1501 caagaagcgc gcgaaggctt ggcagtcctt gcagtagcaa acgacgtgga cgccgctgtc
     1561 tgcggagagc gacaccgacc ctttgagctt tccgcacgca caacgaagag gcaattcggt
     1621 cattgcctct tcgtcatatc actgatcagt ttcgccgccg aggcgtcatg tcgctctccg
     1681 cgcggctcac ctggtcagcg cggtttccgt gtcgattcgg agctcgctca gtggttgact
     1741 tcgcgcagct cgcactcgag gagaaagttc tcggggaacg agccgcgatg caagtcgacg
     1801 aacgcgcgag tcaactcgag cgccgcggct ttgtctttcg cctcgatcat gaaaaaaccg
     1861 ccgatgacct ctttcgcttc ggtgaatggt ccgtcggtga ccgacacttt gccgctcgag
     1921 agccgcactc tcgagccgcg agacgtcggc ttgagccccg ccgccgacaa catgacgcct
     1981 tgttttgccc actcgcccat gagcaagccc atcttcgcgt cgaacccttc aggaagaggc
     2041 tttccggctt cgtagtcagt gttgttctcg agcttgatga caatcagaaa ttgcatgggt
     2101 cgctcctcag gggcgaagtc accccagcgc gttggagggg ttctcgcgtc ctttcatggg
     2161 ggcgacgagc ggaccagctc ccgatcgaca gttcgcgcga tttatttttg ggggcatcga
     2221 ttggccccag ccggccgatc agtcccagcc ggagctaccg gcggcgccga ggattgcgct
     2281 cacgccatag accttgccgc catacatctt gaacaggtgc atgtcggtgt ccgcgttctg
     2341 aaacatagtg aaccctacgc cgagcccagt ctcctcgtcc gcgaagatca aacgaggggt
     2401 cattgcgggg ctaccgctcg ggctgccgga agcgcacgaa gc
//
LOCUS       DASTKS010000071         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1348827, whole genome shotgun sequence.
ACCESSION   DASTKS010000071 DASTKS010000000
VERSION     DASTKS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348827"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFQ35_01475"
     CDS             complement(<1..903)
                     /locus_tag="VFQ35_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9929320.1"
                     /translation="MSGSLRIARLFGIDIRAHVTFVLLLGLAALDFGQSGGARGAVFG
                     VLLVLALFGCITLHELGHSLVARRFGAVVREIVLLPIGGVARLQREPQSPFAELLTAL
                     AGPLVNVVIALGLGALWVARVGKLPEPAHLAEHLARPTPDTFLLWLMASNGLLAVFNM
                     LPALPMDGGRVLRALLSFFVGRARATTIAAGVGQALALGLIAFGLLARPPQLILTLIG
                     LFVFFAAGQERRAVRASDALRELKAGEVCHPAAEALGPSDDLGDAVDHALRTGQSVFP
                     VLYGKDLIGVVLRDEVLMAAGKLGL"
     gene            complement(1326..2039)
                     /locus_tag="VFQ35_01480"
     CDS             complement(1326..2039)
                     /locus_tag="VFQ35_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929321.1"
                     /translation="MHLRVHSAPGFEPAPAWLVSNGDLTVGPVATHLLVRGYLDGRIP
                     PDCLVQPEEGGAWRPLEAVREIRALLEPEASSSPFSLSPPVVVRWLSEARDVSEVMTC
                     ALHGACAFTQATIGALYRVRAPVDLPVLSAVLGEPSIELGEILPRRDPALCLAREGEP
                     TVLLPDSTLAARASALRLCPDRQPKGLAIVPIRAASDIAGMIELARFDHPFRGSEVRA
                     LVPLMAATIARLEELGWDR"
     gene            2229..2618
                     /locus_tag="VFQ35_01485"
     CDS             2229..2618
                     /locus_tag="VFQ35_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9929322.1"
                     /translation="MNSPARCASVEVQPPSGPLPYDRSRLLSQLGGSLEILAEVAELF
                     CLDAERKHAELRGYVLREDQHATGVAHQLKGVLLNVAADDAAATARELETLIRAGQWR
                     AALAATDRLGAQLRELVEAFGQEPKRG"
     gene            complement(2632..>3779)
                     /locus_tag="VFQ35_01490"
     CDS             complement(2632..>3779)
                     /locus_tag="VFQ35_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET9929323.1"
                     /translation="ARRRSFTEGRRLRVLEPSPERVTEACALADTCGGCDWQHISYDA
                     QLELKAGLIAEALGRTGGFRELPAIAVTASPHALGYRLRARFHIDEHGEIGFHEKRSH
                     RVIAVSRCPVAHPELERALSDFRRIASTVPRLLTEFETAELRVSPLTPERALCLLARG
                     EPREARRRASVLLERLEAEFAVAIGGLPANFTQSFPLAEELLLEVSPHGFVQVNWEVN
                     LALVDYVVRGATARGAKRFLDAYAGAGNFTLPLARAGLSGLSIEAQPEGALAAAQSFA
                     RYEFSKLEALADDAAHALARLARERASFDLVLLDPPRAGAQPLLEPLLALQPPHIAYC
                     SCDPVTLARDLRTLCQHGYAIEEIRGFDMFPGTHHVETVVWLGRRCE"
BASE COUNT          720 a         1260 c         1226 g          573 t
ORIGIN      
        1 caagccgagc tttcccgccg ccatcaaaac ctcgtcgcgc aataccactc caatcaagtc
       61 tttgccgtac aacaccggaa agacgctctg cccggtgcgc agggcgtgat ccacggcgtc
      121 acccaagtcg tcactcgggc cgagcgcctc cgccgctgga tgacaaactt cgccggcttt
      181 cagctcccga agcgcgtcgc tcgctcgaac cgcgcgtcgc tcttgccccg ccgcgaagaa
      241 cacgaacaaa ccgatcagcg tcaagatgag ctgcggcggc cgcgccaaga ggccaaacgc
      301 aatgagcccg agcgcgagcg cctgtcccac gcccgccgcg atcgtcgtcg cgcgcgcccg
      361 accaacgaag aacgaaagca gcgcccgcag cacccgaccg ccgtccatcg ggagcgcggg
      421 gagcatgttg aagacggcca agagaccatt cgacgccatc agccaaagaa ggaacgtgtc
      481 cggcgtcggc cgtgcgaggt gctccgctag gtgcgccggc tcgggcagct tgccgacccg
      541 tgctacccac agagccccga ggccgagcgc gatcacgacg ttcaccaacg gacccgcgag
      601 cgcggtcaag agctcggcga aagggctctg cggctcgcgc tgcaatcgag ccacgccccc
      661 gattgggagc aggacgattt cgcgcaccac ggcgccgaaa cggcgagcga ccaagctgtg
      721 gccgagctcg tggagcgtaa tgcaaccgaa cagcgcgagc accaagagca cgccgaagac
      781 agcgccccga gcaccgccgc tctgcccgaa atcgagtgct gccaagccga ggagcagtac
      841 aaaagtgacg tgcgctcgaa tgtcgattcc gaacaggcgc gcaatgcgca gcgagccgct
      901 catgtttttc gcgctcgcgt cgtacggtag gcagtcatct tgcatggacc gtgtggctga
      961 acgcaggacg gcgcaagctg gcccttagag gagccccctg cagacgaagc cgtggcgttt
     1021 gcgtttcggg ttcgaacgct cacttgcaga attgaaagac aatccagccc gcgtgacgtg
     1081 catctagctg aagtctgttc aaaaacacgc ggcacgctcc gtgcactaat tgggggtgca
     1141 ggcggcgccc ttggtgcccc cccctccgcc tcggtcgccg cctccctaat ctcctcagcg
     1201 gcttggagtg cccccctcgc tccaggccgt tgtctttttt gtcgtacgcc acgagatcag
     1261 agctccgctg acgtagcgtc ccatgcgcca ccggcttccc gctgtcgttt acgctcgcga
     1321 cgtgctcagc ggtcccaacc gagctcctcg agccgggcta tcgtggcggc catgagcgga
     1381 accagagccc gcacctcgct gccccgaaag gggtggtcga agcgcgcgag ctcgatcatt
     1441 cccgcgatat ccgaggcggc gcgaatcggt acgatggcga gccctttggg ctgccgatcc
     1501 ggacataggc ggagtgcgct cgcgcgagcg gccagcgtcg aatccggaag caaaacagtg
     1561 ggttcgccct cgcgcgccag gcacaacgct ggatcgcggc gcggcaggat ctcgccgagc
     1621 tcgatcgatg gttctcccaa cactgccgaa aggaccggca ggtcgaccgg agctcgcact
     1681 cgatagagcg ccccgatcgt tgcttgagtg aaagcgcacg ctccgtggag ggcgcacgtc
     1741 atcacctcgc tcacgtcgcg agcctccgaa agccaccgca cgacgacggg cgggctgagc
     1801 gagaacggtg acgacgaagc ttccggctcc aagagcgcgc ggatttcccg caccgcctcg
     1861 agtgggcgcc acgcgccgcc ttcctctggt tgcactaggc agtctggggg gatgcgaccg
     1921 tcgaggtatc cgcgcaccaa caggtgcgta gccacaggcc caacggtgag atccccgttc
     1981 gagaccagcc aagccggggc gggctcgaac ccaggtgcgg agtgaacgcg cagatgcatt
     2041 gaggacagcc tccttgactc gaacgagccg gactttcaac cacagcggtt gggctggcca
     2101 agttccctca ctccactgaa agccaatcca cgagacaggt tagcgtcact ggaattttct
     2161 cggattccac cgctcggcga cgccccctta cctttcatgc gccgcgtccg ctttgacggc
     2221 gagccctgat gaactccccc gcccgttgcg cttcagtaga agttcagcct ccgagcgggc
     2281 cgctgcccta cgaccggtcg cggttgctct cgcagctcgg aggaagcctc gagatcctcg
     2341 ccgaggtggc ggagctgttc tgcctggacg ctgagcgaaa gcacgccgag cttcggggtt
     2401 acgtactgcg cgaagaccag cacgcaaccg gcgtcgctca tcagctcaaa ggggtcctgc
     2461 tgaacgtggc cgctgatgac gctgccgcga ccgcgcgcga gctcgaaacg ctcatccgcg
     2521 cgggacagtg gcgcgcagcg ctcgcggcga ctgaccgtct cggcgcacag ctccgcgagc
     2581 tcgtcgaagc tttcgggcaa gagccaaagc gcgggtgacc cgcggctgcg gtcactcgca
     2641 gcgtcgaccg agccacacca ccgtttcgac gtggtgagtt cctgggaaca tatcgaaacc
     2701 tcgaatctct tcgatcgcgt atccgtgctg gcagagtgtg cgaaggtcgc gcgcgagcgt
     2761 gacggggtcg cacgagcagt aagcaatgtg cggcggctga agggcgagca gcggctcgag
     2821 aagcggttgc gcgccggcgc gcggcgggtc gagcagtacg agatcgaagc tcgctcgttc
     2881 ccgcgcgagg cgagcgaggg catgcgcggc gtcgtccgcg agcgcctcta gcttggagaa
     2941 ctcatatcgg gcaaaggact gggctgcggc gagcgccccc tctggttggg cttcgatcga
     3001 aagaccagag aggccggcgc gggcaagcgg caaggtgaag ttgccggcac ctgcgtacgc
     3061 gtcgaggaac cgcttggcgc cgcgagctgt ggcaccccga acgacgtagt ccacgagcgc
     3121 cagattgacc tcccagttga cctgcacgaa gccgtgggga gaaacttcga gcaagagctc
     3181 ttctgcgagc ggaaaagatt gggtgaagtt cgcagggaga ccgccgatgg ccaccgcgaa
     3241 ctccgcctcg aggcgttcga gcaagaccga agctctccta cgtgcttcac gtggctcgcc
     3301 gcgcgcgagg aggcagagcg cacgctccgg agtcaatgga gagacgcgga gctcggccgt
     3361 ctcgaattcg gtgagcaacc ggggcacggt gctcgcgatc cggcgaaaat cagagagcgc
     3421 tcgttcgagc tcggggtgcg cgaccggaca gcgactgacc gcaatcacac ggtggctacg
     3481 cttttcgtga aagccgatct cgccgtgctc gtcgatgtgg aaacgtgctc gcaaacgata
     3541 accgagagca tgaggcgatg cggtgacggc gatggccggg agctcgcgaa aacctccggt
     3601 gcgccccagc gcttctgcga tgagcccggc cttgagctcg agctgtgcgt cgtacgagat
     3661 gtgctgccag tcacagccac cgcaggtgtc ggcgagcgcg cacgcctcgg tgacacgctc
     3721 aggactaggt tccagcacgc gcagacgccg gccctcggtg aagctacgcc gacgcgccg
//
LOCUS       DASTKS010000072         6330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_901990, whole genome shotgun sequence.
ACCESSION   DASTKS010000072 DASTKS010000000
VERSION     DASTKS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901990"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1010)
                     /locus_tag="VFQ35_01495"
     CDS             complement(45..1010)
                     /locus_tag="VFQ35_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9929324.1"
                     /translation="MIPATAHFIWIGTEFPWLNYAAIASALANGGFSRVVLHHTHDLS
                     GATWWRALARHPAVERRRIIPEEVLRRVGCHGLVELYRELSAPAALTNMLRLTVLLEE
                     GGVYLDLDTVTARSLSALREASFFCGAERIAFPASLERTRNPARWGAAFLRTGVRDVA
                     RRSAHGVHWFRKLEHLYPAAANNAVIGAERGHPFLAELLRRVASMPRKHALQRYALGT
                     SLLQHALGEIDSNALVVHAPEVFYPLGPELSQHWFRLNCRARLDDVLRPATRVVHWYA
                     SVRTRHLIPEITPLWVERNRDRQLLSALLFNALEKEFRSVGEPSA"
     gene            complement(1007..1753)
                     /locus_tag="VFQ35_01500"
     CDS             complement(1007..1753)
                     /locus_tag="VFQ35_01500"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HET9929325.1"
                     /translation="MAVHVSIHDVCPRYTAEIEHAMLLCRARDLAPALLVVPNMHGHA
                     PLDGDPSFVERLLDHERAGSQILLHGLYHLTEVAPDRVRHGALAEVPRYLRQRVASAG
                     EAEFGELSIAEAAERLDRGLSMFSRLGLPVHGFVPPAWILPRRLMPALAARKIRYTES
                     HFGVHDPVSGRSRTSAVMNFATRTHARLLTSVSYVRAARALRGTLPLRIAIHPPDLRS
                     PLALRETIRLLDWARGDFVQRVEDLFEVQL"
     gene            complement(1756..2937)
                     /locus_tag="VFQ35_01505"
     CDS             complement(1756..2937)
                     /locus_tag="VFQ35_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9929326.1"
                     /translation="MKVVDLTEFWSERGGGVRSYLTTKAQSFAELGVEHRVFASGPRS
                     EEITLASSERSSSRLLRFRGPALPYDPTYHLFAQLGAVRRKLGDERPDVLEIHSPQLA
                     ALAGLAAAKRSFRVRTLVWHSDFIDTHLSWKVAAHSSERTARALTEPLWAWVRAVARQ
                     CSATLVASRWQASKLREHGVPRVTEMPFGIDTRVFRPEARDDAFRERFLGRRNAALLV
                     SVGRLAVEKRADVLIEGFRHLRARRDALLLVYGDGPERRDLEARARGLADVVFLGFER
                     DRRELARALASADALVHSGPFETFGLVVGEARACATPVVVADSGAAGELVSPACSEKY
                     AACDPQALAAAIERLLARAPAELAAHARESSRQVLSVSQHFTGLLSFYRELIERSQEA
                     N"
     gene            3272..4702
                     /locus_tag="VFQ35_01510"
     CDS             3272..4702
                     /locus_tag="VFQ35_01510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9929327.1"
                     /translation="MSSPTFVPLAASLRTPHPRHIGIINDYVKIPYANGSSFASQFLY
                     REFTRRGHEVTVFGPRDPKAVGEELPRKAVLFDSLPLHNHPGLFLALPSREALARAVA
                     AKLDVVLAQTGSGLLDLGVWLRAHAGVPLLCVNTIHLPSVYNVLLPDALHARPEVRQL
                     FEAELIPRVERLTASAYNLSDGLVVLSSGLEQYWRERGVTVPIHVIPRSVEPKVFDAP
                     TGADPFPAAARRGARLLCVCRHTREKEVERLIRTFAERIAPNCPEASLTLVGDGPDHD
                     AFVALARRLGVAERVHFPGEFPLTEVTQFYRHADVFVYASLSETYGQVVSEALWNALP
                     VVAFADRMGVSHQVSPGENGELVEPGPDTERADSRFASLVVRLLRDPAQRGAYGQRAR
                     LLARERSNPELAVARYGAAFMSAREHCARTFKPGGASARLVPLARWAAFHTLLAGLGR
                     VRAPAVLNRHGRRQPGWERVTPATAA"
     gene            4711..5607
                     /locus_tag="VFQ35_01515"
     CDS             4711..5607
                     /locus_tag="VFQ35_01515"
                     /inference="COORDINATES: protein motif:HMM:NF039471.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HET9929328.1"
                     /translation="MDAVLPLTWRDLPRARILLRSLEKKFEKLGEIWVVCPDHERSVI
                     ERGLASHPLALTVLEESALVPEFRLTPWLGGWYRQQLVKLAIYEKVKSELYLTLDADV
                     VCAKAVSPELIAPRGLGPCHTVFRDLHPDWYRHSAAVLDMRAKRRGISHNVTPAVLHR
                     VAVGALARYLEERARLRQFARGFRGFKQRLGFAAGGSSSIYEPWRLFLALGAPWTEYA
                     LYYTFLEATGAFERFHEHVDTCLYDVEGSIWRADRRDFAGWRLGANMQHAGAPWFIVV
                     QSNTGISPAAIEAKLFGELSNA"
     gene            5600..>6330
                     /locus_tag="VFQ35_01520"
     CDS             5600..>6330
                     /locus_tag="VFQ35_01520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9929329.1"
                     /translation="MREPQSALRVLARPAFSNRGENPYNWLLYTGLSRLGVQVDEYGI
                     GRSLVGRYDICHVHWPESTFNSSLIEALATTESLLCSLDALRARGTRVVWTVHNLRAH
                     ERRFPRAERAFFDRFVRRVDAFVALSRGSRELAQSTFPALESRPGFIVPHPHYRSEYP
                     DGVARSQARVRLTLSQDEHVLLFFGRVLEYKNVLELIRFVRALPPVVAGKKLCLVVAG
                     RPHGARLERAIYEAAASDPRVRLHL"
BASE COUNT         1068 a         2060 c         2109 g         1093 t
ORIGIN      
        1 gagaagtgag ggcgagtgat ggagaatgtg gacgatgcgc ctcgtcatgc gctcggctcc
       61 ccgacgcttc gaaattcctt ctcgagcgcg ttgaaaagca gcgcgctcaa cagctgtcga
      121 tcccggtttc gctccaccca gaggggagtg atttcgggaa tgaggtgccg ggttcgcacc
      181 gaggcatacc agtgcacgac gcgcgtcgct ggtctcaaaa cgtcatcgag gcgcgctcgg
      241 cagttcaggc ggaaccagtg ctggctgagc tctggcccga gcgggtaaaa gacctccggc
      301 gcatgcacga cgagcgcgtt cgagtcaatc tcgccgaggg cgtgctgaag gagcgaagtg
      361 ccgagcgcgt agcgctggag cgcgtgtttg cgaggcatgc tcgccactcg ccgcaagagc
      421 tccgccagaa agggatggcc gcgctctgcg ccgatgaccg cgttgttggc ggctgcgggg
      481 tagaggtgct cgagcttgcg aaaccagtgc acgccgtggg cggacctccg cgcgacatcg
      541 cgcacgcccg tccgcaagaa ggcagcgccc caccgcgcgg gattgcgggt gcgctcgagg
      601 ctggccggaa acgcgatgcg ctcggcccca cagaagaagc tcgcctcgcg cagcgccgag
      661 aggcttcggg ccgtgacggt gtcgagatcg aggtaaaccc cgccctcttc gagtagcacg
      721 gtcaagcgca gcatgtttgt gagcgctgcc ggggcactga gctcgcgata gagctcgacc
      781 agtccgtggc aaccgacgcg acggaggact tcttcgggaa tgattcgacg gcgctcgacc
      841 gcaggatgcc gcgccagcgc ccgccaccac gtcgcgccag acaagtcgtg agtatggtgc
      901 agtacgacgc gcgaaaaccc gccgtttgcc agcgccgacg cgatggcggc gtagttcaac
      961 cacgggaatt cggtgccgat ccagatgaag tgcgcggtcg ctgggatcac agctgcacct
     1021 cgaacaagtc ctccacgcgc tggacgaagt cgcctcgcgc ccagtcgagc aagcggatgg
     1081 tctcgcgcag cgcgagtggc gaacgcagat ccggcgggtg aatcgcgatg cggagcggca
     1141 gcgtgcctcg caaggctcga gctgcgcgta cgtaggaaac gctcgtcagc aggcgcgcat
     1201 gggttcgcgt cgcgaagttc atcacggcgg aagtgcggct gcgccccgag acggggtcgt
     1261 gaacgccgaa atggctctcg gtgtaacgga ttttgcgcgc cgccaacgcc ggcatcaaac
     1321 ggcgcggcaa gatccaagcg ggcggaacga acccgtgcac cgggagcccg agccgcgaga
     1381 acatcgacag gccacgatcc agtcgttcag cagcctcggc gatgctgagt tcgccgaact
     1441 ctgcctcgcc agccgacgcg acgcgctgac gcaaatagcg aggcacctct gcaagcgctc
     1501 cgtgccggac gcggtcgggg gcgacttcgg tcaagtggta gagcccatgg agcaggattt
     1561 gagagcccgc cctttcgtgg tcgaggagac gttcgacgaa gctcggatcg ccgtcgagcg
     1621 gggcgtggcc gtgcatgttg ggcaccacga gcagggccgg tgccaaatcg cgtgctcggc
     1681 atagcagcat cgcgtgctcg atctccgccg tatatcgcgg gcaaacgtcg tgaatggaaa
     1741 cgtgcacggc catgatcagt tcgcctcttg cgagcgctcg atgagctcgc gatagaacga
     1801 gagtagcccc gtgaaatgtt ggcttaccga aaggacctgg cgggacgact ctcgggcgtg
     1861 cgcggcaagc tccgccggag cgcgagcgag gagccgctcg atcgccgccg ccagtgcctg
     1921 cggatcgcac gccgcatact tttcggaaca cgcgggggaa accagctctc ccgcggcgcc
     1981 ggaatctgcg accacgacgg gcgtcgcgca cgcacgcgct tcgccaacca ccagaccgaa
     2041 ggtctcgaaa gggcccgagt gcacgagtgc atcggcgctc gcgagcgccc gcgcgagctc
     2101 tcggcggtcg cgttcgaaac ccaagaacac gacgtccgcc aagccgcggg cgcgcgcctc
     2161 gagatcgcgt cgctccggtc cgtctccgta gacgagcagc agcgcgtcgc gccgagcacg
     2221 aaggtgccga aagccttcaa tcaagacgtc cgctcgcttt tcgacggcca gtcgcccaac
     2281 actgacgagc agagccgcgt tgcgtctgcc gagaaagcgc tcgcgaaagg cgtcgtcacg
     2341 cgcctcgggg cgaaacactc gcgtgtcgat gccaaaaggc atttccgtga cgcgcggcac
     2401 gccgtgctcg cgcagcttgc ttgcttgcca gcggcttgcg acgagcgtcg cggagcactg
     2461 ccgagccacc gcgcgcaccc aagcccagag cggctcggtg agcgcgcgcg cggtgcgctc
     2521 cgagctgtgc gcggcgactt tccaggaaag gtgagtgtcg atgaagtcgg agtgccacac
     2581 caaggtcctt acgcgaaagc tgcgcttcgc cgcagcaagc ccggccaagg ccgcgagttg
     2641 cggggaatgg atctcgagca cgtccggtcg ctcgtcgccg agcttgcggc gcacggcgcc
     2701 gagctgggca aagaggtgat aagttgggtc gtaggggagt gcagggccgc gaaatcgcag
     2761 cagccgcgag ctcgagcgct cgctactcgc cagagtgatc tcctcgctgc gcgggccgga
     2821 agcgaacacg cggtgctcga caccgagctc ggcgaaggac tgcgccttgg tggtcaggta
     2881 ggagcgcacg ccgcctcctc gttcggacca gaattcggtt aagtcgacga ctttcatgtg
     2941 acaccagcac tgaagccccg ttgcgacgaa agcagcacgg acacgtcgcg gtctcgaggc
     3001 gttgacgcga acgatgcgtt cctaccgtgc acgaagcgtg cgagatgcga acgggcgcgt
     3061 cggtctcgcg ttcctgtcgc cggagctgca cacgaacgag cgccgttcgt agctaaacgt
     3121 gaaatgggag cacaccgtga aatgctgctc tggggtgaga aaggcacata agggcgcggg
     3181 ctcgtcgcgt tttgcggcaa ctcagcggtt ttggttttgg ccgagcattt gcaatccgcg
     3241 tcgtggtctt tcgatcgcga ggaaccgatt catgtcatct ccgaccttcg ttccgttggc
     3301 ggcttcattg cgaacgcctc atccacgaca catcggcatc atcaacgact acgtgaagat
     3361 tccgtatgcg aacggctcct ctttcgcgtc gcagtttctc tatcgcgaat tcacacgacg
     3421 cggccacgaa gtcacggtgt ttggcccgcg cgaccccaag gcggtgggtg aagagctgcc
     3481 acggaaagcg gtcttgttcg actcgctccc gttacacaac cacccggggc tgttcttggc
     3541 tttgccttcg cgcgaagcgc tggcgcgagc cgtcgccgcc aaactcgacg tcgtcttggc
     3601 ccaaaccggc tccgggctct tggacctcgg agtttggttg cgtgctcatg cgggggtgcc
     3661 gctcttgtgc gtcaatacga ttcacctgcc gagtgtctac aacgtgctcc tgcccgacgc
     3721 gctgcacgcg cgccccgagg ttcgacagct cttcgaggcc gagctcatcc cgcgggtcga
     3781 gcgcttgacc gcgagtgcgt acaacctcag tgatggactc gtagtgctct cgtccggcct
     3841 cgagcagtat tggcgtgagc gcggcgtgac cgtgccaatc cacgtgatcc cgcgttcggt
     3901 cgagcccaag gtattcgatg cgccgaccgg cgcggatccg ttccccgctg cagctcgacg
     3961 cggggcgcgg ttgctctgcg tgtgccgcca cacccgtgag aaggaggtcg aaaggctaat
     4021 ccgcaccttt gccgagcgaa tcgcccccaa ctgtcccgag gcgtcgctga cgctggtggg
     4081 ggacggtccc gatcatgacg cgttcgtggc gctcgcgcgg cgactcggtg tcgctgagcg
     4141 cgtgcacttc cccggcgagt tcccgctgac cgaagtgacc cagttctacc gacacgccga
     4201 cgtgttcgtg tacgcctccc tctccgaaac ctacgggcaa gtcgtcagcg aggcgctgtg
     4261 gaacgcgctc ccggtagtgg cgttcgccga tcgaatgggc gtttcgcatc aggtcagtcc
     4321 cggcgagaac ggggagctcg tggagcccgg gccggacacg gagcgggccg actcccgctt
     4381 tgcgagcctc gtagtgcgcc tgctccgcga ccccgcgcag cgtggggcat acggtcagcg
     4441 cgcgcgactc ctggcgcggg agcgctcgaa ccccgagctt gcggtcgcgc gctacggtgc
     4501 ggcgttcatg tcggcgcgcg aacactgcgc gcggaccttc aagcccggcg gggcatccgc
     4561 gcgcctcgtg ccgctcgcgc gctgggcagc attccatacg ctgctcgccg ggttgggccg
     4621 tgtgcgtgcc cctgccgtgc tcaatcggca cgggcgacgc cagcccgggt gggagcgcgt
     4681 tacgccggcg accgcagcct aggaggagcg atggacgccg tattaccgct cacttggcgc
     4741 gacctaccac gcgcccgcat tctccttcgt tctctggaaa agaagttcga aaagctcggg
     4801 gagatctggg tggtgtgccc cgatcacgag cgctctgtga tcgagcgtgg gcttgcgagc
     4861 cacccactcg cgctgacggt gctcgaggag tcggcgctag tgcccgagtt tcggctgact
     4921 ccttggctcg gaggatggta ccggcagcag ctcgtcaaac tcgcgatcta cgagaaggtg
     4981 aagagcgaac tttacctgac cctcgatgcc gacgtggttt gcgccaaagc agtgagtccc
     5041 gaactcatcg cgccgcgggg cctcgggccc tgtcatacgg tcttccgcga cttgcacccc
     5101 gattggtatc ggcactccgc cgcggtgctc gacatgcgcg ccaaacgtcg tggcatctca
     5161 cacaacgtaa cgccggcggt gctacaccgc gtcgcagtag gcgcgctcgc tcggtacctc
     5221 gaagagcgcg ctcgcctgcg ccaattcgca cgcggatttc ggggcttcaa gcaacggctc
     5281 gggttcgcgg cgggaggctc ttcgtcgatt tacgagccgt ggcgactgtt cctcgcgctc
     5341 ggcgcgccct ggaccgagta cgcgctctat tacacgttcc tcgaggcgac cggcgccttc
     5401 gagcggttcc acgaacacgt cgacacctgt ctctacgacg tcgagggttc gatctggcgc
     5461 gccgatcgcc gcgatttcgc gggatggagg ctcggcgcga acatgcagca cgcgggcgct
     5521 ccgtggttca tcgtggtgca atcgaacacc gggatcagtc cggctgccat cgaggccaag
     5581 cttttcgggg agctctcgaa tgcgtgagcc gcagtctgcg ctccgggtgc tcgctcgccc
     5641 ggcgttctcg aaccggggag agaaccccta caattggctg ctctatactg gcctctctcg
     5701 tctcggcgtc caggtcgacg aatacgggat cgggcgcagc ctcgtcgggc gctacgacat
     5761 ctgccatgtg cactggccag aatcgacttt caattcttcg ctcatcgagg cgttggcaac
     5821 gaccgagtcg ctgctctgtt cgctcgacgc gctccgcgcg agaggcacgc gcgtcgtctg
     5881 gacggtgcac aaccttcgcg ctcatgagcg gcgtttcccg cgcgccgagc gggcgttctt
     5941 cgaccggttc gtacgtcggg tagatgcctt cgtcgcgctt tctcgcggct cgcgcgagct
     6001 cgcccagagc accttcccgg cgctcgaatc gcggccgggc ttcatcgtgc ctcatccgca
     6061 ctaccgctcc gagtatccgg acggcgtcgc gcgctcgcaa gcccgtgtcc ggcttacact
     6121 gagccaagac gagcacgtgc tgctcttctt cgggcgagtg ctcgagtaca agaacgttct
     6181 ggagctcatc cgattcgttc gtgctctgcc gccggtcgtc gctgggaaga agctttgcct
     6241 cgtcgtggcg gggcggcctc acggagcacg gttggagcgg gcgatctacg aggctgccgc
     6301 ttccgaccct cgcgtgcgtt tgcacctcaa
//
LOCUS       DASTKS010000073         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1571932, whole genome shotgun sequence.
ACCESSION   DASTKS010000073 DASTKS010000000
VERSION     DASTKS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571932"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFQ35_01525"
     CDS             complement(<1..230)
                     /locus_tag="VFQ35_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929330.1"
                     /translation="MAINLKRVLVGGLVAGLTVAVCGAALVPIVGDEMREALSRLRLP
                     PLTPGAMAYFGLVSLVIGLVLVWLYAALQPRR"
     gene            468..1304
                     /locus_tag="VFQ35_01530"
     CDS             468..1304
                     /locus_tag="VFQ35_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929331.1"
                     /translation="MVRAPISSPASIFRTLRSITIWVGSAVALALVGLCLVRANVPNE
                     LLKSSSDAVGNYLQTLGTIYAVLLAFVVFVVWQQFNDARAHIEGEANELLDLARTVHG
                     LPETLWRPFLSHVKRYVELVLAEEWPVMAGRGNATFARGSQLLDEMWELLVEYEPCSE
                     CHKSLYDEALARFNDLSDRRMSRLVSSRLRIPLALRLLLYTGAVITAASMWLFYVESF
                     AVHAVMTSALAGCISHVLYVISDLDDCFSGNWQVPSSQFERVGAQVEARLERAPEKGR
                     SR"
     gene            1416..>3664
                     /locus_tag="VFQ35_01535"
     CDS             1416..>3664
                     /locus_tag="VFQ35_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9929332.1"
                     /translation="MIDGALARSRDGLGALVLFTGEAGIGKSRLVREAAERAAAAGLR
                     VAWGNCWEVGGAAPYWPWVQVFRSLERAPFDVPLPETHDFIQQRFKLFDSATRELAEL
                     ARREPLVILLDDLHAADLPSLHFLSFVARQLAALPVALIGSARIPEFYAAPGVNDLLL
                     GIMREGERVSLARLSREAVAKWVAGTADSEAVYAATEGNPLFVKEVLRLGAVARGPND
                     TIAKVLDAHLRLLPADAHGLLAMASVFGRQISVQELAALAGTSSDALREHLNTACSLG
                     VLEPLDGSRLQFTHVLLRERLYEALRERSALEWKAGLLLETRGADPALVAHHLLAGVD
                     AGDVARAGRAALRAIEHAQTTLAFEFAAAFAEEVLARLGTSLPAPLACELEVAFGEAL
                     MRAGSFSGGRAQCVRAAERARSLGHAEMQARAALAYGSEMLAASVDPVMVRLLEDAKS
                     ALGTGDSALGARVGARLAAALIPPATPEEAKRVQALSHAALATARRIGDRSALLWTLL
                     FVRAALIALIHDDEVFALTEELVDLARSLEQRDVLMIVGPKHAVALLERGRRAEARAA
                     LAELVETAAEVRYPLLAWRVPMLRAAFAMFDGRLDEARELGEEALALAQKAGTQQANV
                     VWAQQRIAIAIARREPASIAPDADRVLSMLRTNPLLSPIRAWVLAACGSPREALQELE
                     SLSERDQAFGLVFAAETCLLLESCPRDGEAMPDSLAALAARVRPLLSERWHGTRFFFG
                     ALAAGFVVGP"
BASE COUNT          554 a         1153 c         1245 g          712 t
ORIGIN      
        1 ttgcgtcgag gttgcagggc agcgtacagc cataccaaca cgaggccgat caccagcgat
       61 acaagtccga agtaggccat tgcgccgggg gtgagcggcg gcaagcgaag acggctcagc
      121 gcctcacgca tctcgtcgcc gacgatgggt accagtgctg cgccgcacac ggctaccgtc
      181 aacccggcga cgaggcctcc gacgagcacg cgcttcagat tgattgccat gatatcgctc
      241 cccgtttcgg cccgctgctt tggtgcggtc aagcagcgcc caacgttagg gtttcccggc
      301 gtgttcggat ccgtagagag caaggaacgc accgcgcgtg attgagctgt ggttcgcggt
      361 ggcgcggcgc agaagctgca gtgaagtcgg tgcggtcgtg caccgcgtgc gtgggtggac
      421 gcgacggaac gcgttgcccg accggttcgg actatactcg catcgaaatg gtccgcgctc
      481 ccatctcttc tcccgcgagc atctttcgaa cgcttcggtc gattacgatt tgggttgggt
      541 ccgcggtggc gctggctctc gtcggcctgt gcctcgtgcg agctaacgtt cccaacgagc
      601 tcttgaagag ctcgagcgat gcggtcggca actacttgca aaccctcgga accatttacg
      661 cggttctgct cgccttcgtg gtgttcgtcg tgtggcaaca attcaacgac gctcgcgctc
      721 acatcgaagg ggaggcgaac gagttgctcg acctggcccg caccgtgcac ggcttgcccg
      781 agaccctgtg gcgacctttc ttgagccatg taaagcgcta cgtcgagctc gttctggcgg
      841 aggagtggcc ggtgatggct ggacgcggca acgccacgtt cgcgcgcgga agtcagctgt
      901 tggacgaaat gtgggagctc ttggtcgaat acgagccctg ctcggaatgc cacaaatcgc
      961 tctacgacga ggccttggct cgtttcaacg acttgtccga ccgacgcatg agccgcctgg
     1021 tttcgagccg cttgcggatc ccactcgcgt tgcgcctctt gctctatacc ggcgcggtca
     1081 tcaccgccgc gtcgatgtgg ctcttttatg tcgaaagctt tgcggtgcac gcggtcatga
     1141 cgtcggcgct cgctggatgt atctcacacg tgctctacgt catttcggac ttggacgatt
     1201 gcttcagcgg caattggcag gtgccgtcgt cccagttcga acgggtggga gcgcaggtcg
     1261 aagctcgcct cgagcgcgct cctgagaaag gccgctcgcg ttgatggtca actcgatgca
     1321 gaaccaggta ttggtgccgc gcctgaggta acctagagtt cacgtcggac gggagctcga
     1381 cccaaatgct ttttggccgc gaaccagagc tgagtgtgat cgacggcgct ctcgctcgtt
     1441 cgcgcgatgg tttgggtgcc ctcgtgctct tcacggggga ggcgggcatc ggcaagtcgc
     1501 gtctggtgcg cgaagcagca gagcgagcgg ccgcggcggg cctgcgagtc gcgtggggga
     1561 actgctggga ggtgggcgga gcggcaccat actggccttg ggttcaagtc ttccgttctc
     1621 tcgagcgcgc tcccttcgac gtgccgctcc cggaaacgca cgactttatc cagcagcggt
     1681 tcaagctgtt cgatagcgca acgcgagagc tcgccgagct cgcacgtcga gagccgctcg
     1741 tgattctgct cgacgacctg cacgcggccg acttgccgtc tctgcatttc ctgtcgttcg
     1801 tggcgcgtca actcgctgcg cttccggtcg cgctgatagg ttctgccaga atcccggagt
     1861 tttacgccgc gccgggcgta aatgatctgc tgctcggaat catgcgagaa ggggagcgcg
     1921 tctcgctggc acggctgtcc cgcgaagcgg tggcgaagtg ggtggcgggt acggcagatt
     1981 cggaggcggt gtatgcggcg acggagggca accccctctt cgtcaaagag gtgctccgct
     2041 tgggcgcagt cgctcgcgga cccaacgata ccatcgcgaa ggtactcgac gcgcatcttc
     2101 ggctgttgcc cgccgatgcg catgggttgc tcgcgatggc ttcggtcttc ggccggcaga
     2161 tttcggttca ggagctcgcg gcgctcgccg gaacatcgag tgatgcgctg cgcgagcacc
     2221 tcaacacggc gtgttcgctc ggcgtgctcg agccgctcga cggatcacgc ttgcagttca
     2281 ctcacgttct cttgcgcgag cgcctctacg aagcgcttcg cgagcgctcc gctctcgagt
     2341 ggaaagcggg gctgctcctg gaaacgaggg gcgccgatcc ggctctcgtt gcgcaccatc
     2401 tactcgccgg cgtcgacgcc ggcgacgtcg ctcgggccgg gcgcgcggcc cttcgtgcga
     2461 tagagcacgc gcagacgacg cttgcattcg agtttgcggc tgcgttcgcc gaggaggtgc
     2521 tcgcgcgcct cggtacctct ctgccggcgc cgctcgcctg cgagctagag gtcgcgttcg
     2581 gagaggcact gatgcgcgcg ggttcgttca gcggtggacg agctcagtgc gttcgcgcgg
     2641 cagagcgcgc tcgtagcctc gggcacgccg aaatgcaggc gcgcgccgcg ctcgcctacg
     2701 gctcggagat gctcgcggcg agcgtcgacc cggtgatggt ccgccttctc gaggacgcga
     2761 agagcgcttt gggaactggt gactcagccc tcggggcgcg ggtcggtgcc cggctcgcgg
     2821 cggcgttgat tccgcccgcg acgccagaag aagcgaaacg cgtgcaggcg ctctcccacg
     2881 cggcgctcgc caccgcgcgc cgcatcggtg accggagcgc gttgctttgg accctgctct
     2941 tcgtgcgcgc cgcgctgatc gcgctgattc acgatgacga ggtgttcgca ttgaccgaag
     3001 agctcgtcga cttggcgcgt tcgctcgagc agcgcgatgt cctgatgatc gtcgggccaa
     3061 agcacgcggt cgcattgctc gagcgcgggc ggcgggccga ggctcgcgct gcgctcgcgg
     3121 agctcgtcga aacggcggct gaagttcggt atccactgct ggcatggcgc gtccccatgc
     3181 ttcgcgctgc gtttgccatg ttcgacgggc gcctcgacga agctcgggag ctcggggagg
     3241 aggcgctggc gctcgctcag aaggcaggca cgcagcaagc gaacgtcgtg tgggcccaac
     3301 aaaggatcgc gattgcgatc gcgagacgtg agccggcgag catcgcgccc gacgccgacc
     3361 gggtgctctc gatgctccgg acgaacccgc tcttgtcacc gatccgggct tgggtgctcg
     3421 cggcctgcgg gagtcctcgc gaagcgttgc aggagctcga gagtctttcc gaacgggatc
     3481 aggctttcgg cctcgtcttt gccgcagaga cttgcctatt gctcgaatcg tgcccgcgcg
     3541 atggagaggc catgcctgat tcgctcgccg cgttggccgc gcgcgttcgc cccctcctga
     3601 gcgaacgctg gcacggcacg cgcttcttct tcggcgctct cgcggctggg ttcgtcgtcg
     3661 gacc
//
LOCUS       DASTKS010000074         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_679702, whole genome shotgun sequence.
ACCESSION   DASTKS010000074 DASTKS010000000
VERSION     DASTKS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679702"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFQ35_01540"
     CDS             <1..988
                     /locus_tag="VFQ35_01540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9929333.1"
                     /translation="GAVKALKAQHRICLTGTPVENHLGELWSLFDFLMPGFLGDNERF
                     RVKYRTPIERAGNQAVLESLRHVVAPFILRRMKEHVARELPPKTQIVRPVELEGDQRE
                     LYESIRVAAHGEVRNLIRKKGIAGSTILILDALMKLRQVCCDPRLVSSSAAGDVQSSA
                     KYQLFFDLLELQLSQGRKILVFSQFTRMLALLSAGLNERGIPFVDITGATQNRERQVD
                     RFQGGEVDVFLISLKAGGTGLNLTRADTVIHYDPWWNAAAQSQATDRAYRIGQTQPVF
                     VYDLIVSGSVEERMLRLQQRKSELSRTLLGGPGPTSALSEDDLEDLFAPLAD"
     gene            1241..2713
                     /locus_tag="VFQ35_01545"
     CDS             1241..2713
                     /locus_tag="VFQ35_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929334.1"
                     /translation="MTTDLPGVSAMSLYVPRFRVPLDAWCGWTGNNWDKVRAVVGHSF
                     RVCGQHENVYTMAANAALRLILQNDIDPRRVGFFGLGTESSTDNAAGAVIVRGMLDRA
                     LEALGLPRLSRNLEVPEFKHACLGGIYGLKNALRYVAFDGRDKLAIVVSADVAEYERG
                     STGEQTQGAGAVAMLVERKPRLFAVDLFHAGSASDYRGPDFRKPFARHFTEGYAAKTK
                     RLSDFPVFSGDYSTYSYIDETVHAVEDMLQRLGVSAGSYYRGVHSLFFHRPYHLMPVR
                     AMSFLYVRGLARGDHHHDELRELCAEAKVNFEDVMREASSSPDLFAHVLSGNIQVDPY
                     AVTSAVAAVLRKRQGFRELLAQKMSLGGDVTRSLGNLYSAALPAWVAAGLEEGAEKSM
                     PLASRDMVAVGYGSGDAAEAIPITPVEGYEQAALRIGMRRALEAPIDLTQAQYESLHD
                     HRELRDMDYRPESEFVISRVGTSYETAFQDLGVEYYDYVR"
     gene            complement(2810..3631)
                     /locus_tag="VFQ35_01550"
     CDS             complement(2810..3631)
                     /locus_tag="VFQ35_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929335.1"
                     /translation="MKANRLSACLLGFAVASVSSLAVAAPPDVPPPPSEPPSAPPPET
                     PATAAAAEPPPAAVAPTAPPEAIADRPPLASPGRKIDLSTPPPEGSNPRSYHMHDGFY
                     LRASVGFGSLRATFADDRVSNDSLEGAGASLHLNLLIGGSPSPGLALGGALLAESAAS
                     VDYDYNGRHAYDRSTSLLVLGPFVDGFPKPHRGWHLGGSLGLAQLSLGKTSLDDRRQT
                     YGVGGSFWLGGDFWVAPDWSVGPLLKFTGAVTGASDPDITAQSFSVALLFTVLYH"
     gene            complement(3796..>4356)
                     /locus_tag="VFQ35_01555"
     CDS             complement(3796..>4356)
                     /locus_tag="VFQ35_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9929336.1"
                     /translation="PQTRRSGTGDPSSARAKSRRGVHLAVALGGVAATLGLLWQLGKR
                     QTARTAPVSSATVAPAPPRAAVISIESNPAGARVTEQGRELGVTPLKLSVPNADFERP
                     RTFVLTLAGYRAHVFERAPGPEATYVHVDLVREAPVEAAPPISTPSAPPAQRPVAGPP
                     SPRVRAPRPAAQPSKPESAPDIRLVR"
BASE COUNT          773 a         1468 c         1420 g          695 t
ORIGIN      
        1 cggggccgtc aaggcgttga aggctcagca ccgcatctgt ctgaccggaa ctccggtcga
       61 gaatcatctc ggggagctct ggtctctgtt cgacttcttg atgccgggct tcttggggga
      121 caacgagcgc ttccgagtga agtaccgcac gccgatcgag cgcgcgggta accaagccgt
      181 cctcgaatcg ctgcgtcacg tagtcgcgcc gttcatcttg cggcgcatga aggagcacgt
      241 cgcgcgcgag ctcccaccga agactcaaat cgtgcggccg gtggagctcg aaggcgatca
      301 acgcgagctc tacgagagca tccgagtcgc tgcgcacggc gaggtccgaa acctcatccg
      361 caagaagggc atcgccggct cgacgatcct catcctcgac gcgctgatga agctccgtca
      421 ggtctgctgc gatccacggc tcgtgagcag cagcgccgct ggcgacgtgc agagctcggc
      481 gaagtaccag ctcttcttcg acttgctcga gctgcagctg agccaaggcc gcaagatctt
      541 ggtgttctcg cagttcacgc gcatgctcgc gttgctctcg gcggggctca acgagcgagg
      601 gatccccttc gtggacatca ccggcgcgac gcaaaaccgc gaacgtcagg tcgaccgctt
      661 ccagggcggt gaagtcgacg tcttcctcat tagcctcaag gcgggcggta ccgggctgaa
      721 cctcacgaga gccgacacgg tgatccacta cgatccttgg tggaacgcgg cggcccagtc
      781 gcaagcgacc gatcgcgcct atcgcatcgg tcaaacgcaa ccagtgttcg tgtacgatct
      841 catcgtatcg ggtagcgtcg aagagcgaat gctgcgtctt cagcagcgaa agtcggagtt
      901 gtcgcggacg ctcctcggcg gccccggccc aacgagcgcg ctcagcgaag acgacttgga
      961 agacctgttc gccccgctcg cggactaaga aagctcccga gtttcgccct cgcgcgtggc
     1021 aacgaacgcg cgcgagcgcg cgcccggcag actcaagggt tcgccaccgc ccgccgcagc
     1081 gcagcaatcc ctcgcgtccc ctcgatttcg catccccaca cgagccccct ccccgcgcga
     1141 tgagccgcgt cggaggcttc gcactgccgt cgaccgtgct gcgacccgct ctgtccaact
     1201 tgccggagcc gagcctcggc attagacatc tcccaactcg atgactaccg atctgcccgg
     1261 cgttagtgcc atgagcctgt acgtgccccg gtttcgcgtc ccgctcgatg cgtggtgcgg
     1321 gtggaccgga aacaactggg acaaggtgcg ggccgtggtc ggccatagct tccgcgtctg
     1381 cggccagcac gaaaacgtct acaccatggc cgcgaacgcc gcgttgcgcc tcatcctgca
     1441 aaatgacatc gatccaaggc gagtcgggtt cttcggtctc ggtacggagt cgagcaccga
     1501 caacgccgcc ggcgccgtca tcgtgcgcgg catgttggac cgcgctctcg aagcgctcgg
     1561 gctgccgcgc ctgtcacgca acctcgaagt gccggagttc aagcacgctt gcttgggcgg
     1621 catctacggg ctgaaaaacg cgcttcgcta tgtcgcgttc gatgggcgcg acaagctcgc
     1681 catcgtggtc agcgccgacg tcgccgagta cgagcgaggg agcaccggcg agcagacgca
     1741 aggcgccgga gcggtggcga tgctggtgga gcgaaagccg cggctcttcg cggtggacct
     1801 cttccacgcc ggcagcgcat ccgactaccg cggccccgac ttccgcaagc cgttcgcgcg
     1861 gcacttcacc gaaggttacg cggccaagac caagcgcctg agcgacttcc ccgtgttcag
     1921 cggcgactac tcgacgtact cgtacatcga cgaaacggtc cacgccgtcg aggacatgct
     1981 gcagcggctc ggcgtctccg ccggcagcta ttatcggggc gttcatagcc tttttttcca
     2041 tcgcccctat cacctcatgc cggtgcgggc gatgtcgttc ctctacgtcc gcggcctcgc
     2101 gcgcggcgat catcatcacg acgagctgcg cgagctctgc gccgaggcga aggtgaactt
     2161 cgaggatgtc atgcgcgaag cgagctcctc gccagatctc ttcgcgcacg tgctgagcgg
     2221 caacatccaa gtcgatcctt acgcggtgac gagcgcggtc gcggcagtgc tgcgcaagcg
     2281 ccaaggcttc cgcgagctcc tcgctcagaa gatgagcctt ggcggtgacg tcacgcgctc
     2341 cctcggcaac ctctacagcg ccgccctccc cgcgtgggtc gcagccgggc tcgaagaagg
     2401 cgccgaaaag tcgatgccgc tcgcgagccg cgacatggtc gcggtgggtt acggcagcgg
     2461 cgacgccgcc gaggcaatcc caatcacccc cgtggaaggc tatgagcaag ccgcgctccg
     2521 gatcgggatg cgacgcgccc tcgaagcgcc gatcgatctg actcaagccc agtacgagtc
     2581 actgcacgac catcgcgagc tccgcgacat ggattatcgc cccgagtcgg agttcgtcat
     2641 cagccgcgtc ggcacgtcct acgagaccgc ctttcaggat ctcggcgtcg agtactacga
     2701 ctacgtgcgc tgaacgcgcg cgcgtaccct cgggccgctt gggcccgtca tctcgctcac
     2761 ttctggcaat tcgagcggtg caacgcggaa gcgtccgcgt tgcgttcgct cagtggtaga
     2821 ggaccgtaaa caagagcgcg accgagaagc tttgagccgt tatgtcggga tcactcgctc
     2881 ccgtaacggc acccgtgaac ttcaagagcg gccccaccga ccaatcgggc gcgacccaaa
     2941 agtccccacc gagccagaac gacccgccaa cgccgtaggt ttgtcggcga tcgtcgaggc
     3001 tcgtcttgcc gaggctcagt tgagccaaac cgagcgagcc gcccaaatgc caaccgcggt
     3061 gcggcttggg aaagccatcc acgaatgggc ccaagaccag gagcgacgtg ctgcgatcgt
     3121 aggcgtgacg gccgttgtag tcgtagtcga ccgaggccgc gctctccgcg agcaacgcgc
     3181 caccgagcgc gagccccggg ctcggcgaac cgccaatcag caaattcagg tgcaacgagg
     3241 cgcccgcacc ttcgagcgag tcgttggaga cgcgatcatc cgcgaatgtc gcgcgcaacg
     3301 aaccaaaccc gacgctcgcc cggagataga agccgtcgtg catgtggtag ctgcgcgggt
     3361 tcgacccctc ggggggaggc gtcgagagat cgatttttcg ccctggtgag gcgagcggag
     3421 gtcgatccgc gatcgcctcg ggtggagccg taggcgcgac cgcggcaggc ggcggctcag
     3481 cagcagcggc agtagccggt gtctccgggg gcggagcgga cggcggctcg gaaggcggcg
     3541 gcgggacgtc gggcggagcg gcgacagcca agctcgaaac ggacgcgacc gcgaacccca
     3601 ataagcaagc cgaaaggcga ttggctttca tgacggcacc caagagttag caccttttga
     3661 gtcggcgccc ggccgcgacg caacggcacc acggacgcca ctccacgaag tcgcggaaaa
     3721 ctacctcgag tacccttccg ctcggcgttc gacgcttggg cgccctcgcc tattcatggg
     3781 ccgcggccta gtcggtcagc gaaccaagcg gatatcgggc gccgactcgg gcttgctcgg
     3841 ttgagccgca gggcggggcg cgcgaactcg cggagagggc ggtccagcga ccggacgctg
     3901 cgccggggga gcgctcggcg tcgagattgg cggcgctgct tcgaccggcg cctcgcggac
     3961 gaggtcgacg tgcacgtatg tcgcttcggg ccccggggct cgctcgaaca cgtgcgctcg
     4021 gtaaccagcc aaggtgagca cgaacgtgcg cggtcgctcg aagtcggcgt tcggaaccga
     4081 aagcttgaga ggagtaaccc cgagttcacg cccctgctcg gtcacgcgcg cccccgccgg
     4141 gttgctctcg atcgagatga cggcggctcg aggcggtgcc ggcgcaaccg tcgcggatga
     4201 cacgggagcc gtccgtgcgg tttggcgttt tccgagctgc cacaacaggc cgagcgtcgc
     4261 cgcgacgccg ccgagcgcga cagccaggtg cacgccgcgc cggctctttg ctcgagcgct
     4321 gctcggatcg ccggtccccg aacggcgcgt ctgcgg
//
LOCUS       DASTKS010000075         6919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1794776, whole genome shotgun sequence.
ACCESSION   DASTKS010000075 DASTKS010000000
VERSION     DASTKS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794776"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFQ35_01560"
     CDS             complement(<1..1008)
                     /locus_tag="VFQ35_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9929337.1"
                     /translation="MGKSLFQKVWDQHTVQVLPNGQAQIFIGLHLIHEVTSPQAFGMM
                     RDLGLRVTHPERTFATVDHIIPTDTRLRPFRDNLAEGMIVALEKACKDFGIRFFDVSS
                     GHQGIVHVIGPELGLTQPGMTIACGDSHTSTHGAFGAIAFGIGTTQVRDVLATQTLSL
                     APMKVRRIEVKGKLGPGVYAKDLVLHIIRVLGVNGGTGYAYEYAGEAVHALSMEERMT
                     LCNMSIEGGARVGYVNPDQVTFDYLKGREYAPKGEAWDRAVEYWKSIASDADASYDDV
                     VRIDAKDIGPTVTWGINPGQAISVKENVPSAEDGKTAAEQASIREALEYMMLTPAAPI
                     EG"
     gene            1123..2064
                     /locus_tag="VFQ35_01565"
     CDS             1123..2064
                     /locus_tag="VFQ35_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9929338.1"
                     /translation="MDPALIPALYDVMMVARVGSVAVAARRLNKTPSAVSQQIRRITD
                     ALGVVLFERRGRGLALTQAAEHVFPAATRLFDEAEAVFGLFGELSGSAVTTLRIAASD
                     YLGKPLLVPVLRDLAAEGVPLHFEISTVHSEESLALLERGAVEMAIVSQAGATDRSGL
                     SGSTLLEQDLFWVSPKRAPIKRGARRLPLSERLTKGPVLRLAPGSLGRKLLDRHLEQH
                     AIRPASTVDVPSVSLLLAYATGGVGIGLVPALSLDGHDLGTLLVEPSELARLPVQLVT
                     RSGRPLVPVVERFTARLLQQAERERTRLDQILASRAR"
     gene            2233..2309
                     /locus_tag="VFQ35_01570"
     tRNA            2233..2309
                     /locus_tag="VFQ35_01570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2267..2269,aa:Arg,seq:acg)
     gene            complement(2516..2896)
                     /locus_tag="VFQ35_01575"
     CDS             complement(2516..2896)
                     /locus_tag="VFQ35_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9929339.1"
                     /translation="MAGLTLDTGALIGFERSDRRLLLHLKTAQMRGHELTVPTPVVAE
                     AWRGGARSARIDRLLDVCVIEPLFEDLARLAGEAIASISGAGVIDAIVMASAARRGDR
                     VLTADFNDLARLRAHFPTVRLVTV"
     gene            complement(2884..3057)
                     /locus_tag="VFQ35_01580"
     CDS             complement(2884..3057)
                     /locus_tag="VFQ35_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929340.1"
                     /translation="MQQEAKRSGRTLSAEVTEALGRELRRRRLTQLIAEYEAEHGQIT
                     AEELASIDAEWQD"
     gene            4073..5191
                     /locus_tag="VFQ35_01585"
     CDS             4073..5191
                     /locus_tag="VFQ35_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9929341.1"
                     /translation="MPHSANATTVKSSSQGKDGSLRRRAAFTQRPSFTQRASSGESEQ
                     RAQRSKAKEGTTESSKESRRTASDTLRTHRVKPGETLDGIALHYDTSAALLASANGLK
                     DNDVIRSGQVLVVVHESEALPARSESNSAASSSGGVTIATAAVPPRVDRNASWARYVK
                     APKTRGWVDLSTTMARFSGATLDRHGRLEPEAVRALNNLLNAGGAHPRVPERLLYLLV
                     RVSDTFGGRPIRIVSGYRTSSYYEDSRHKHAAAVDFSITGVPNAVVRDFIREFDDVGV
                     GFYPNSSFVHLDVRTQAAYWVDYAGPGEPPRSTPDAPTRLPRSGARKLLAELDGLFEH
                     ATKTMERIRAKPSRDTSGELARGSESERDQPERDTTSL"
     gene            complement(5234..>6919)
                     /locus_tag="VFQ35_01590"
     CDS             complement(5234..>6919)
                     /locus_tag="VFQ35_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9929342.1"
                     /translation="PLVFDLLVYLVRHRERVVPKEELLSKLWEGTTVTDGSLQRAISL
                     LRATLREGGMEQAVQTFARRGYRFCETPEVPAATLAAERPQDDEALRATRLIAAGQWD
                     QALEAFEASPGKSTLTAQDLEAWGNSALCAGLPERAISPLERAVAAFETAERRSDAAR
                     VALLLANVKLEGRELAIAKGWYQRALSYLRGEPEGKQHGIAEWLASRLALFEGQLDEC
                     RIRARSAMDIGDRIGDPDLHCLGLVYQGHILIAQSDVRRGLSLHDEAGAAALAGRVSP
                     WVGGIVFCSVIWAYLHLGDHHRAGQWTDEFTRWCERHAAYCYPGLCRLHRGEVLAIRG
                     ELETAEAEVRRARQQLATAGPFCEGDACRVLGEISLARGDLDAAESAFREAHRLGWNP
                     QPGLSLLLAARGEHEPAIKQLERALSQPSWTDGQRRGSILAVLARIAATSGRVERARA
                     ALTELASLPELTASRANAAELSRAEAELLWADGDKAHAEPSFRASIAHWLEMNAPLHA
                     ADVRLRLFELLLDAGDPTAAELELSSAERAFEQVGATPLIDRCRALRARIARR"
BASE COUNT         1180 a         2286 c         2238 g         1215 t
ORIGIN      
        1 gccctcgatc ggcgcggccg gcgtcagcat catgtactcg agcgcctcgc gaatcgaagc
       61 ttgctcggcc gcggtcttgc cgtcttcagc gctcggaacg ttctccttca cgctgatggc
      121 ctgaccgggg ttgatgcccc aggtcaccgt ggggccaatg tccttcgcgt cgatgcgaac
      181 gacgtcgtcg tagctcgcgt ccgcgtccga tgcgatcgac ttccagtatt cgacggcgcg
      241 atcccaggct tcgcccttcg gggcgtactc gcggcccttc aggtaatcga acgtcacctg
      301 atcggggttc acgtaaccga cgcgcgcgcc gccctcgatc gacatgttac agagcgtcat
      361 gcgctcttcc atgctgagcg cgtgcacggc ctcgcccgcg tattcgtagg cgtagccagt
      421 gccgccgttc acgccgagca cgcgaatgat gtgcagcacc aaatccttcg cgtaaacgcc
      481 gggcccgagc ttccctttga cctcgatgcg gcgcaccttc atcggcgcga ggctcaacgt
      541 ttgcgtcgcg agcacgtcgc gcacctgcgt cgtgccgatg ccgaacgcga tcgcgccgaa
      601 cgcgccgtgc gtcgacgtgt gcgagtcgcc gcacgcgatg gtcatgcccg gctgcgtgag
      661 cccgagctcc ggaccgatca cgtgcacgat gccttggtgc cccgacgaca cgtcgaagaa
      721 gcgaatgcca aagtctttgc acgccttctc cagcgccacg atcatcccct cggcgaggtt
      781 gtcgcggaac ggtcgcaggc gcgtgtcggt cggaatgatg tgatccaccg tcgcgaacgt
      841 gcgctccgga tgcgtcacgc gcagcccgag gtcacgcatc atcccgaacg cttgcgggct
      901 cgtgacttcg tgaatcaagt gcagcccgat gaagatctgc gcctgcccgt tgggcagcac
      961 ttgcacggtg tgttggtccc agactttctg aaataggctt ttgcccaagg tggcctcctg
     1021 gtgccgcggg gagtgggagt atcacgcgag ccaacactta agtccaatga aacttactgg
     1081 attgtaacat tagcaatgct aaatcgagcg agaggctaac ccgtggatcc agcgctgatt
     1141 cccgctctgt acgacgtgat gatggtggcc cgcgtgggct cggtcgcggt tgcggcgcga
     1201 cgactgaaca aaactccatc tgccgtcagc caacagattc ggcgcatcac cgacgcgctc
     1261 ggcgtcgtcc tgttcgagcg ccgaggtcgc ggactcgcac tcacgcaagc cgccgagcac
     1321 gtgttccccg cggcgacgcg cctcttcgac gaagccgaag ccgtgttcgg cctcttcggc
     1381 gagctctctg gctccgccgt cacgacgttg cgcatcgccg cgagcgacta cctcggcaaa
     1441 ccattgctcg tccccgtcct tcgtgatctc gcggcagaag gcgtgccgct ccacttcgaa
     1501 atctccaccg ttcactccga agaatcgctc gcgttactcg aacgcggcgc ggtcgaaatg
     1561 gcgatcgtct cgcaagcagg cgcaaccgac cgctctggcc tcagcggttc gacactgctc
     1621 gagcaggatt tattttgggt ttccccaaag cgcgcaccga tcaaacgcgg ggcacgccgc
     1681 ctgccgcttt ccgagcggct taccaaaggc cccgtgctcc ggctcgctcc gggcagcctt
     1741 ggtcgcaaac tcctcgatcg gcatctcgaa cagcacgcca tccgccccgc ttccaccgtc
     1801 gacgtcccga gcgtctccct ccttctcgcc tacgccaccg gcggcgtcgg cattggtctc
     1861 gtccccgccc tctcgctcga cggccacgat ctcggcaccc tcctcgtcga gccctccgag
     1921 ctcgcgcgcc tccccgtcca actcgtcact cgctccggtc gccccctcgt ccccgtcgtc
     1981 gagcgcttca ccgcgcgcct cctccagcaa gccgagcgcg aacgcacccg cctcgaccaa
     2041 atcctcgcct cacgcgcgcg ctgaatcctc ggctcgctga atcctcgcct cacgcgcgcg
     2101 ctgagcgcgg tcgaggccgc aaccgaaacc gccggccctt ggcccagtat cgttgaaatc
     2161 accgcgggtc ttgacagagc gcagccgatg agggaaaccc cggcaccctt gccggctagc
     2221 tccggcggga acgcacccgt agctcagctg gatagagcat cggtctacgg aaccgagggt
     2281 cggaagttcg aatcttcccg ggtgcgccat ccccttcggg tctgtcgatc ccgtgaagct
     2341 ttagcgatgt cgcccgcgca agcgcgtgtt tggcgacgaa tcttcccggg tgcgccacga
     2401 acgaaggcag ccaaagtgcc tgggcttttt cgggctggtg gaagagaacg ctcagctcgt
     2461 gggcttctac tgacgcggaa gttcagctgc tctttcgggt tggggagcaa tcggtttaga
     2521 ccgtcacgag ccgaacggtg ggaaagtggg cgcgcaggcg agccaggtcg ttgaagtctg
     2581 ctgttaggac gcggtcacct ctgcgcgcgg cggatgccat gacgatggca tcgatcacgc
     2641 cggcgcccga gattgaggcg atggcctcgc cagcaaggcg cgcgaggtct tcgaacagcg
     2701 gctcgatgac gcacacgtcg agcagtctgt cgattcgagc agaacgagca ccgcctctcc
     2761 acgcctcggc gacgacaggc gtaggaacgg tcagctcgtg cccgcgcatc tgagcggtct
     2821 tcagatgcag caacaaccgt cggtcggaac gctcgaagcc gatcagcgct ccagtatcta
     2881 aagttagtcc tgccattcgg catcaattga ggcgagctct tcggcagtta tctgcccgtg
     2941 ctcagcttca tattcggcga tgagctgggt cagccggcga cgacgcaatt ctcggccaag
     3001 agcttcggtc acctccgcgc tcagagtacg tcccgagcgc ttggcttctt gctgcattgc
     3061 tcgaagtacg ccttcgtcta cggtcagaga aactttcttg gcccggccac gcggcgaaga
     3121 gcgcacagaa cgccgcgctc ctgctggctc tcggccagaa gctgaccgtt tcctcgcagt
     3181 agacttaagc acccatttat cctaccggag gtaggatgct gcggcaaccg caagtccgaa
     3241 gccgcgaacc gagcgtgctg aacgtcaacc cgccaggacg tgcgcagttc taaattcatc
     3301 tgcttgggtg cgccatccgc ttcgggtctg tcgattcggt gaagctttag aggcttcgct
     3361 ctcgctgcgg gcacgaatgg gaacgaatct tcccgggtgc gctaggaacg agagccaacg
     3421 gctcttggga ttttggccgg tgcgtggaag cggcgggcgg cttccgctaa acgtcctgca
     3481 tctgttgggc tcggcacatg aggcgccgct cacttggtgg tgctcgtgcg cgaaggtgaa
     3541 gggggtttcg gcaagggatc gttgagcggc gacggttcgt cctgcgtctc aggacacgca
     3601 tggcgcgcag ctcggcactc ttcgtacgtc cgccaggaag gcgtggcgtg ctggccatcg
     3661 cattcgcata cggtgtccgc caggcagcgc gcgccgtccc agtagtaacc tctaataacg
     3721 ccaaacccgc agcacgaagc gacaatcccc cgatgcggag gggtcgatgg cgcgcagtcc
     3781 gacctgcctg accggcaccc cgacgcagtc gcaatcagtg cgatgccagt cacgatgatc
     3841 gtgaccatgc acttcattcg ctgcccgcac ccagacgttc ccatgccgct aggttagagc
     3901 agcgaggacg agggcagttc aagtggtggt tagcctgaag ctttccgcga taggtgcgcg
     3961 ccgctgctcc aaacgcgcca gcaaagttgg cccaatgtag gtaattgtgt gagctggcgg
     4021 ggtgaggtct cggcggcttt tcaaactcgt gtgccttgcg ggcctgctcg ctgtgccgca
     4081 ttcggcgaat gcgacgacgg taaagagctc gtcacagggc aaggatggct cgctgcggcg
     4141 gcgcgcggcg ttcacgcagc gcccgtcgtt cacgcaacgt gcgtcgagcg gggagagcga
     4201 gcagcgcgcg caacgctcga aggcgaagga agggacgacg gagtcgtcca aggaatcgcg
     4261 gcgcaccgca agcgatacgc tgcgcacgca ccgagtgaag cccggggaaa cgttggacgg
     4321 catcgcgctt cactacgaca cgagcgcggc gctactagcg agcgccaatg gcctcaaaga
     4381 caatgacgtg atccgctcgg gacaggtgct cgtcgtggtt cacgagagcg aggcgttgcc
     4441 ggcgcgctcc gagtcgaaca gtgcggcgag ctcgagcggt ggggtgacca ttgcgaccgc
     4501 ggccgtgccg ccccgggtcg atcgaaacgc gagttgggcg cgctacgtga aggcgccgaa
     4561 gacgcgagga tgggtcgatc tctcgaccac gatggcgcgc ttttcgggag ccacgctcga
     4621 ccgacacggc aggttggagc ccgaggcggt gcgcgcgctg aacaatctgc tcaacgccgg
     4681 cggtgcccac ccgcgcgtcc ccgagcggct tttgtacttg ctggtgcgag tgagcgacac
     4741 gttcggcggg cgcccaattc gcatcgtgag cggctaccgc acgagctcct actacgaaga
     4801 ctcgcggcac aagcacgcgg ccgcggtcga cttttcgata acgggcgtac cgaacgcggt
     4861 cgtgcgcgac ttcattcgcg agttcgacga cgttggcgtg gggttttatc cgaactcgtc
     4921 cttcgtccat ctcgatgtgc ggactcaggc ggcgtattgg gtcgactatg ccggtccagg
     4981 tgagccaccg cggtcgacac ccgacgcgcc gacgcggctc ccgcgcagcg gcgcccggaa
     5041 gctactcgcc gagctcgatg gattgttcga gcacgcgacc aaaacgatgg agcgcattcg
     5101 cgcaaagccc tcgcgcgata cttccggtga gcttgcgcga ggaagcgagt ccgagcggga
     5161 tcagccggag cgcgatacga catcactgtg atgagcttgc gcgaggaagc gagttcgagc
     5221 gtccggacgc gcgctagcgt cgagcgatac gcgcacgcag cgcgcgacac cggtcgatga
     5281 gcggagtcgc gccgacttgt tcgaaagcgc gctcggccga gctgagctcg agctcggccg
     5341 cggttggatc gcccgcgtcg agcaagagct cgaacaggcg cagtcgcacg tcagcggcgt
     5401 gcagaggcgc gttcatttcg agccaatgcg cgatgctcgc gcggaacgag ggttcggcgt
     5461 gagctttgtc gccgtccgcc cacaagagct cggcttcggc gcgcgaaagc tcggccgcgt
     5521 tggcgcgcga agcggtgagt tcgggaagag acgcgagctc ggtcaaggcg gcccgagccc
     5581 gctctacgcg gccgctcgtg gccgcgatgc gcgccagcac cgccaaaatc gagccgcggc
     5641 gttgtccgtc ggtccagctc ggttgtgaga gagctctctc gagctgcttg atcgcgggtt
     5701 cgtgctcgcc gcgggccgcg aggagcaagc tcaagcccgg ctgcggattc catccgaggc
     5761 gatgcgcttc gcggaaggcg ctctccgcgg cgtcgagatc gccgcgcgcg agagagatct
     5821 cgcccaagac tctgcaagcg tcaccttcgc agaaggggcc ggccgtagcg agctgctggc
     5881 gagcgcgtcg aacctctgcc tcggcggtct cgagctctcc gcggatcgcg agcacttctc
     5941 cgcggtgcaa gcggcagagg ccggggtagc agtacgcggc gtggcgctcg caccagcggg
     6001 taaactcgtc agtccattgg ccggcgcggt ggtggtctcc caagtgcaaa taggcccaaa
     6061 tcaccgagca gaagacgata ccgccgaccc agggactcac gcggccggca agggccgcgg
     6121 ctccagcttc gtcgtgcagc gagaggccgc gacggacgtc ggactgcgcg atcaggatgt
     6181 ggccttggta gacgaggccc aagcagtgca agtcggggtc gccgatgcgg tccccgatgt
     6241 ccatcgccga tcgagcgcga atgcggcact cgtcgagttg tccctcgaag agcgccaaac
     6301 ggctcgccaa ccattcggcg atgccgtgct gcttgccttc gggctctccc cgcaagtagc
     6361 tgagcgcgcg ctggtaccag cccttcgcga tcgcgagctc tcggccttcg agcttgacgt
     6421 tggcgagcag aagcgcgacg cgcgcagcgt cgctgcgtcg ttcagcggtt tcgaaggcgg
     6481 cgaccgcgcg ctcgaggggc gagatggcgc gttccggcaa gccagcgcaa agcgccgaat
     6541 ttccccaagc ttccaagtct tgcgcggtca gcgtcgactt gcccggcgag gcctcgaacg
     6601 cctcgagcgc ctggtcccac tgccccgccg cgatcaagcg cgtcgcgcgg agcgcttcgt
     6661 cgtcttgggg tcgctccgcg gcgagcgtcg ccgccggcac ctccggcgtt tcgcagaagc
     6721 gataaccgcg tcgcgcgaag gtttgaaccg cttgctccat ccctccctcg cgcagcgtcg
     6781 cgcgtagcag gctgatcgcg cgctggagcg aaccgtcggt gacagtggtg ccttcccaga
     6841 gcttcgacag gagctcttct ttgggcacga cgcgttcgcg gtgccgcacc aggtacacga
     6901 gcaggtcgaa gacgagggg
//
LOCUS       DASTKS010000076         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_233180, whole genome shotgun sequence.
ACCESSION   DASTKS010000076 DASTKS010000000
VERSION     DASTKS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233180"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFQ35_01595"
     CDS             <1..744
                     /locus_tag="VFQ35_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9929343.1"
                     /translation="EELSTVNDELQARNAQLTELNSDLGNLLASVQIPIVMVSADLRI
                     RRFTPAAERMLNLIPSDVGRPIAHLKPNISCPDLEDLIRHVVETVSVREREVSDVDGN
                     TFLLRVRPYKTVDNRIDGAVLALFELSTSLRLARETGEALMARLLEPALLVDARLIVR
                     SANPAFLTLIGGEARDVENHCLFDIDASWDMPELRALLERDLPSIQGFRLTLTSPRFG
                     RRELLLDARYVAHVHSGITLIIFREPGSS"
     gene            complement(722..2350)
                     /locus_tag="VFQ35_01600"
     CDS             complement(722..2350)
                     /locus_tag="VFQ35_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929344.1"
                     /translation="MSHKSRVSPSSPTHEAQSARSEESEAQRLRRLTERLVDERRAAE
                     LTRDEYVDLFDLAPLPGLTLDAAYITRRMNQAMCALLGVVKERCIDQSFRGFVLPDDR
                     RLLSEHLARTPYAPTARCRLHLLPSSSEPIPVELWSNVMGRSGLYQLCVVDLRQEVLS
                     AFEAGRLVEAERSARSESAAKDVFIAMLSHELRTPLTPALAVASFFRESHASRDARRA
                     FSIIERNLHAEVRMIDDLLDLNRVVRGKMAVTCRALSVHDVLCEAAEELRPLANAKHQ
                     TLALDLRAENQYASVDGLRLRQVFGNLIRNAVKFTDQAGNICVRSWNQGSRLVVEVED
                     DGIGIAPEELHRLFTPFDQLAVRPGAVGGLGLGLAISRGLLELQGGRLSVQSAGLGRG
                     SRFVVELETVPEPEATGPSRAEAQGVSPVLARVRRILLVEDDPDSAEVLAELLVASGF
                     EVQTAASVRAARAVDMTMVDLIVSDIGLPDGSGLDLIRELQADRHRPAVALTGFGRES
                     DVVAAVEAGFDAHLTKPIDIHRLLEMLIKMSQAL"
     gene            complement(3235..3420)
                     /locus_tag="VFQ35_01605"
     CDS             complement(3235..3420)
                     /locus_tag="VFQ35_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929345.1"
                     /translation="MAALTSVDLLILDDFALEPMSKEESRDVYQLFLERTGRASMIVT
                     SNRDTGEWIAACPRWRS"
     gene            3572..>3827
                     /locus_tag="VFQ35_01610"
     CDS             3572..>3827
                     /locus_tag="VFQ35_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929346.1"
                     /translation="MSAMYADPIEAAETPSARASAHAAEVEGILSSDEMIRKSHSDLE
                     AMLDGQGKEWARLMLEENLRLRAQLERETAVVGADGVERKS"
BASE COUNT          663 a         1250 c         1258 g          656 t
ORIGIN      
        1 gaagagctca gcacggtgaa cgacgagctc caagcacgga acgcgcagct gacggagctg
       61 aacagcgacc tcggcaacct gctcgcgagc gtgcagatcc cgatcgtgat ggtgagcgcc
      121 gaccttcgca ttcggcgctt cacgcccgct gcggaacgca tgctcaactt gatcccgtcc
      181 gacgtggggc ggccgattgc ccatctcaaa cccaacatca gttgcccgga cctcgaggac
      241 ctcatccgcc acgtcgtcga gacggtcagc gttcgcgagc gcgaggtctc cgacgtcgac
      301 ggcaacacgt tcctcctgcg cgtgcgtccg tacaagacgg tagacaatcg catcgacggc
      361 gccgtgctcg cgctcttcga gctctcgacg agcctaaggc tcgcgcgcga gaccggcgaa
      421 gcgctcatgg cgcggctgct cgaaccggcc ctgctggtcg acgcgcgcct gatcgtgcgc
      481 agcgccaacc ccgccttttt gacgctcatc ggcggtgagg cgcgtgacgt cgagaatcat
      541 tgcctgttcg acatcgacgc gagctgggac atgccggaac tgcgggcctt gctcgagcgg
      601 gatctgccga gcatccaggg cttccgcctg actctcacgt caccccgctt cggacggagg
      661 gagctcttgc tcgacgctcg ctacgtggct cacgtacatt cgggcatcac gctaatcatc
      721 ttcagagagc ctggctcatc ttgatgagca tctcgagcag gcggtggatg tcgatgggct
      781 tcgtgaggtg cgcgtcgaag cccgcttcga ccgcagccac gacgtcgctc tcgcgtccga
      841 agccggtcag cgcgaccgcc ggacggtggc ggtcggcttg gagctcgcgg atcaaatcca
      901 gcccgctgcc gtccggtagg ccgatgtcgc tgacgatgag atccaccatc gtcatgtcca
      961 cggccctggc ggctcgaacg gacgcggccg tttggacctc gaagccgctc gcgaccagga
     1021 gctcggcgag tacttcggca gagtccggat cgtcctccac gagcaggata cgcctgaccc
     1081 tagccagcac cggggatacg ccctgagctt cggcacgcga gggtccggtc gcttccggct
     1141 cggggaccgt ctcgagctcc acgacgaagc gactgccacg accgagcccc gcgctctgca
     1201 ccgaaagcct cccgccctgg agttctagca gaccacgact gattgcgagg cccagaccta
     1261 ggccgccgac cgcccccgga cgcacggcga gttggtcgaa cggcgtgaac aggcgatgaa
     1321 gctcttccgg tgcgatgccg atcccgtcgt cctcgacttc gacgacgagg cggctcccct
     1381 ggttccacga ccgcacgcag atgttcccag cctgatccgt gaacttgacg gcgttgcgaa
     1441 tcaagtttcc gaacacctgg cgcaggcgca acccatcgac gctcgcgtac tgattttcgg
     1501 cgcgtaaatc gagcgctagg gtctggtgct tggcgttcgc gagcgggcgc agctcttccg
     1561 ctgcttcgca aaggacgtcg tggaccgaca aagcacggca agtgaccgcc atcttgccgc
     1621 gcacgacgcg gttcagatcg agcaggtcgt cgatcatgcg cacttcggcg tgcagattcc
     1681 gttcgatgat ggagaacgcc cggcgagcgt cccggctcgc gtgcgactcg cgaaagaagg
     1741 aggcgactgc gagcgccggc gtgagcggcg tgcgcagctc gtggctgagc atcgcgatga
     1801 acacgtcctt cgcggcactc tcgctgcgcg ccgaacgttc tgcctcgacg agtcggcctg
     1861 cctcgaacgc gctgagcacc tcctgccgca ggtcgacgac gcaaagctga tacagaccgc
     1921 tgcgccccat cacgttgctc cagagctcca ccggtatcgg ctcgctcgaa ctcgggagca
     1981 ggtgcaagcg gcaacgcgcc gtcggagcgt acggggtgcg cgcgaggtgc tcggagagca
     2041 gccgcctatc atcggggagc acgaagccgc gaaagctctg gtcgatgcac cgctccttga
     2101 ctacgccgag gagcgcgcac attgcctggt tcatccggcg cgtgatgtac gccgcgtcca
     2161 acgtgagccc tggcaaaggc gccaaatcga acaaatcgac atactcgtcg cgcgtcaact
     2221 cggccgctcg gcgttcatcg acgaggcgtt cggtcagacg tctgagccgc tgggcttcgg
     2281 attcctcgga gcgggcgctc tgcgcctcgt gcgtcggcga cgaaggcgaa acgcgcgatt
     2341 tgtgactcat gatcggtcaa ccaaggtttg caccgccccg aatgacgagc gctgagcaat
     2401 gcccatcacc gaatttttcc tgcaccattc ggttgctcct ctgctgccgc ctgcgtacca
     2461 cgcgtcctac tcagtggaaa cccgaggaaa ctccaagttc gctcgctcgc gcactcaagt
     2521 tctgtatatt cgactctgac ccctgtcggg acaatttgca ccggcccgcc gtagagcggc
     2581 tctttgcttc gaacccctca gcgtgcacac tggccatcgc gcggtccgtt cgacgctggt
     2641 cgcgagagaa ccgcctgacc gaactatgag ctgcgcgggc ttttgagcga tcccaagaac
     2701 tggtgcgcgc cggggcgtgg cgccggttcc gtcgagaatc atcgacagag aaagtccaat
     2761 ggagaacgac atgaagaagt ccgaacgagc cggcgtgccg cccgcagcaa ctcggaccgc
     2821 ccggtcttcc gctaccggcg cgcgcgcgct accgggatcg ttcggcgaag cggcgagcgt
     2881 cgcgcaggtt ttcggcgccg agcgctggtc cttcgacgac gttctgcggc gtgtcctcgg
     2941 tttctctcgc gcggcggaga gcgaacgcga cgttctcaac cggcgcgctc gccaggcgtc
     3001 atcgcggtcg ccgaagcgga ccggaagcga tgtcgggacg ccgagtccgg cattcgaggc
     3061 acttcgtatg ttcgttcgat cgctcgaccc cgaaacgcag tacaagctgc gcgcgttgtc
     3121 gcgtgccggt cgcgaggcac gagcgctgcc tggagctttg gctgccctgg ctgccgaacg
     3181 ggcgagcggt tccgcgggcg cgtccgagct gttcgtggag ggcgcgacgg agcttcagga
     3241 cctccagcga ggacacgccg cgatccactc gccggtgtcg cggttgctgg taacgatcat
     3301 cgaagcgcgg ccggtgcgct cgaggaagag ctggtaaacg tcgcggctct cctccttgct
     3361 catgggctcg agcgcgaagt cgtcgaggat cagcaagtcg acggaagtga gcgcggccat
     3421 ctcggcgtcg cgggagttgt cgaagcggct cgggtgcaga tcgaccgcgg cggcctgaaa
     3481 tcgtgtaatt ctctggggtt cggggaatta acgacgacgg cgtgaggttt tggcggcgag
     3541 ggcgtttttt gccctcgaag ggagccgtgc catgagcgcc atgtacgccg atccaatcga
     3601 ggccgctgag acgccgtccg cgcgcgcgag cgcgcatgcc gcggaagtag agggtatcct
     3661 gtcgagcgac gagatgatcc gcaaatccca ctcggacttg gaggcaatgc tcgacgggca
     3721 gggcaaggaa tgggcgcggc tgatgctcga ggagaatctg cggcttcggg cccagctcga
     3781 gcgggagacc gcggtcgtcg gtgcggacgg ggtggagcgc aagtcgg
//
LOCUS       DASTKS010000077        12404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1126241, whole genome shotgun sequence.
ACCESSION   DASTKS010000077 DASTKS010000000
VERSION     DASTKS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126241"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFQ35_01615"
     CDS             complement(<1..397)
                     /locus_tag="VFQ35_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9929347.1"
                     /translation="MNTRRPQEEAVPSLLDVVARASRALKASIQVPLERHGVHAGQNF
                     ILERLWEHDGQTPGEIAEAIGVEGPTVVRALQRMAASGLVTRKKHPSDGRQVVIHLTP
                     AGARLRAQIPRAMRRVEEQALAGFSNAERA"
     gene            1023..1340
                     /gene="tnpB"
                     /locus_tag="VFQ35_01620"
     CDS             1023..1340
                     /gene="tnpB"
                     /locus_tag="VFQ35_01620"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9929348.1"
                     /translation="MIEAFIPASATRVVVYLQPVNVRWGVDKLRVFCRDEMRMEPNDS
                     TCFLFVNRARDTLLLYHLGYSGEQTLIKKLDKGSFVLPAPEENGRRFVILRPVMLGRL
                     FRS"
     gene            complement(1690..1881)
                     /locus_tag="VFQ35_01625"
     CDS             complement(1690..1881)
                     /locus_tag="VFQ35_01625"
                     /inference="COORDINATES: protein motif:HMM:NF040165.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929349.1"
                     /translation="MDALLAHDREHARQLTPSEKLQQALEVMRTGIRMKRSTLRRQFP
                     EVEEAEIDRLLAEWLRADG"
     gene            complement(2143..3054)
                     /gene="sucD"
                     /locus_tag="VFQ35_01630"
     CDS             complement(2143..3054)
                     /gene="sucD"
                     /locus_tag="VFQ35_01630"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9929350.1"
                     /translation="MAILVNKNTRVVVQGITGSAGSFHAKQCIAYGTQIVAGCTPGRG
                     GETFASEGPGGKAISVPVFDTVAQAVKQTGADTSCIFVPALGAADAILEAVDAGCKLV
                     VCITEGIPVMDMIRVRRAIDGKTVGGQPVRLIGPNCPGIITPEACKIGIMPGHIHRAG
                     NIGVVSRSGTLTYEAVGQLTALGLGQSTCIGIGGDPVGGMDFVDVLTLFNEDPDTHAI
                     MMMGEIGGNAEERAAEYIKKNVKKPVAAFIAGTTAPPGKRMGHAGAIISGGKGTASDK
                     IKALENAGVKVAPSPAEMGSTLKSLLK"
     gene            complement(3070..4269)
                     /gene="sucC"
                     /locus_tag="VFQ35_01635"
     CDS             complement(3070..4269)
                     /gene="sucC"
                     /locus_tag="VFQ35_01635"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9929351.1"
                     /translation="MKIHEYQGKQIFAKYGIAVPSGIPCFNGGDVEAAATKLLAETGG
                     SVVVVKAQIHAGGRGKAGGVKVVKGGPAEARKAAEAMLGMQLVTHQTGPEGQKVRRLY
                     VEQGLDIARELYLGLVVDRETRRIAVMASTEGGVEIERVAEETPEKILIEHIDPVIGL
                     SGFQARKIGYGLKITDGVPNPKETFAEFIKTLTKLVELFEKEDCSLCEINPLIVTKQG
                     KIVALDSKINFDDNASGRHREWNELMDRDEEDPVELEAKEAGLNYVALDGDIGCLVNG
                     AGLAMATMDIIQHHGGSPANFLDVGGGATKEQVTKAFKMILSSPKVKGIFVNIFGGIM
                     KCDVIAEGVVAATRELGLKVPLVVRLEGTNVEKGKQILRDSGLAIQPADGMADGAKKI
                     VSATRAA"
     gene            complement(4356..5294)
                     /gene="mdh"
                     /locus_tag="VFQ35_01640"
     CDS             complement(4356..5294)
                     /gene="mdh"
                     /locus_tag="VFQ35_01640"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9929352.1"
                     /translation="MARKKISLIGAGNIGGELANLAVTKSLGDVVLFDIPAKENFAKG
                     KALDLEQASAISGADSRVVGTSKWEDVAGSDVLIITAGIPRKPGQSRDDLVATNLPII
                     RDVADAAKKHCPDAFVIVISNPLDAMVYEYKRRTGGSRQKIVGMAGVLDSARFSLFLA
                     REAGCSVKDLRTMVLGGHGDDMVPVLSHCTVNGVPIQKFVKQEALDKIVDRTRKGGGE
                     IVGLMGTSAYYAPAISAIAMAEAYLLDQKRVLASAVYLEGEYGYKDLYMGVPAVIGSG
                     GVEKIIELELNDAEKAMLKKSADSVQKVVDIVKAST"
     gene            complement(5312..6700)
                     /gene="icd"
                     /locus_tag="VFQ35_01645"
     CDS             complement(5312..6700)
                     /gene="icd"
                     /locus_tag="VFQ35_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET9929353.1"
                     /translation="MALFEGPKSGDAITLASGKLSVPDQPIIPFIEGDGTGPDIWRAS
                     QAVLDASVQKAYGGKKKIAWYEVYAGEKSKKKFDSWLPDETVEAYRKYLVGIKGPLTT
                     PVGKGIRSLNVALRQQLDLYVCLRPVRWFTNVPSPVKRPGDVDMVIFRENTEDIYAGI
                     EWASGTPEAKKLLTFLQTEMGVKKIRFPETSGFGIKPVSKEGTERLVRAAIQYALTFG
                     RKSVTLVHKGNIMKFTEGAFRDFGYEVAKREFRSQIVTEREAWILGNLEEKPSLSAEE
                     NAKLIEPGYDMAPPETQAELRAEVDAAAALKATHGDGKWKKLLRVKDSIADITLQQVL
                     TRPKDFDVIATLNLNGDYLSDALAAQVGGIGIAPGGNINYDTGHAVFEATHGTAPKYA
                     NLDRVNPGSVILSGEMMLRHMGWVEAADLIIKGMDGAIGAKTVTYDFARLMEGAKEVK
                     CSEFGRAIIDHM"
     gene            6894..7529
                     /locus_tag="VFQ35_01650"
     CDS             6894..7529
                     /locus_tag="VFQ35_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929354.1"
                     /translation="MFRTDRDRARLLRLGLAATLSCCACTGAPLPRSDAAQRAAGEPN
                     QIKVDPCRNEAGKPPPAALARAYQGLLATARCVAEVDVMMQEVSAALGVRCDYCHVGT
                     DYARETEKSRIANFMATELVPRLDAKDHTEVTCVRCHRGVAKILGAPRSEPRALEWMT
                     AELSPNFVTKDDRPLRCKSCHGADWGTPGFHRTVILTHELDGLPRAAAKSP"
     gene            7526..7879
                     /locus_tag="VFQ35_01655"
     CDS             7526..7879
                     /locus_tag="VFQ35_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9929355.1"
                     /translation="MTERARLFVYGSLKRGFANHRVLRNADFLGECRTAPHYRLFDLG
                     AYPALATDGALSITGELYSYPVEHFSELDAFEGPAYRRGTVALSDGSTAEAYLLVTAE
                     CDEAIELHTDTWRAR"
     gene            7933..8367
                     /locus_tag="VFQ35_01660"
     CDS             7933..8367
                     /locus_tag="VFQ35_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929356.1"
                     /translation="MTSPIQSMVIPRPMHVVIVDDDPEVVTGLRRVLHSAEPEWLIST
                     ANNGKEALSLMSGEKVDVVITDLHMPEMDGFQLLGQLERAHPETIRIVHSSHTATLAT
                     QLMRYLAHNVLTKPTPARDIVAMARWAVHAAGAVRRYEACEA"
     gene            complement(8386..10125)
                     /locus_tag="VFQ35_01665"
     CDS             complement(8386..10125)
                     /locus_tag="VFQ35_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929357.1"
                     /translation="MQRVDFFGLERPIQERFVAAARGTAPPIPLAIKRQLMPRVGVIC
                     LALAALALVTMLALTRVGYAKLDSSFALQPTGALVLYVVLGVACGALLGVAFRIQRAR
                     SRWPIAPAVYAFPAGVVDARSLNMEVHSLRDLVEATASGRKLNLAFSDGARFQFDTTD
                     ANRALELARAVNGFREYFMPDAAPVSARERAVWDPLADNGFSNPFSPRERIKKPKSDL
                     SWVAVPLAAIVLAGFGALVFTVRNSLGERALYAVARKVDTRAGYEAYLERGGSNLDVA
                     TLLLPRAQLAEVVKKQSIDELVQFVKAHAGEPVDVEAQSALLKQLLAALEDAKRAGSL
                     RALKEFRARFADQPAILGEVTRAINARTEAALARFVEKAHPKPEVLDAFRRLVAYGVE
                     HDGVVQVRSKRRLPDSVAKSEELLQKAHWYGGKASLPGQYFDAAHAAQRERPTQKQII
                     EALSQAFDEDVLKFESGPAYDDNTDEDPKVTVPTILIIHRTEMSGAYLMKNPRAAITG
                     VGVLFRISMLLPGAGSVHSFKYSAWNSPDVKSMMEGRPFEEIYDDMADKSFAKLTKKY
                     LAEVVPGLAPKAQ"
     gene            10324..11679
                     /gene="gor"
                     /locus_tag="VFQ35_01670"
     CDS             10324..11679
                     /gene="gor"
                     /locus_tag="VFQ35_01670"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET9929358.1"
                     /translation="MDALYDFIVIGGGSGGLAAARRARRYGARTVVVEPHPLGGTCVN
                     RGCVPKKILWNASELADKLVDLPDYGFEPRPRGAFDYARLREASRAYVSRMNANFAVR
                     LDEEGVELVRATARFLEPTVVETSDGRVLRAPHVLVATGSRPAWPRVPGAELGISSDD
                     WFGLDQLPRRLLVVGGGYIGVELAGIAHSLGSDVVLTFRGEAPLARFDDMLRTTLADE
                     MQRVGITQVAGFRPARVSRSGPGGLQVEGEDGRLLEDFDCVLWATGRVPNVESLALEK
                     AGVALNAAFAIGTDAFQNTSMPGVYAVGDVTGVSMLTPVAITAGRKLADRLFGGQPDA
                     RLDYEDIPTVVFSHPPIGTVGLSEAMARARYGDQVKTYVTRFTGMYYGITQRKPRTAM
                     KLVTLLPDERVLGIHAIGLGADELIQGFAVALRMGARKSDLDRTVAIHPTAAEELVTM
                     R"
     gene            complement(11713..>12404)
                     /locus_tag="VFQ35_01675"
     CDS             complement(11713..>12404)
                     /locus_tag="VFQ35_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929359.1"
                     /translation="SGPWQAMDAVSFAWSWVTKDFGGVALPLAVGWFVMLLPQSLLGG
                     ARGFAQSFLLTSGAIDADTATLIGLIMTPISAAVGVLSQAYFLGGITRFALTVARGRK
                     PEFGEVFAGGSTFGAMFVGQLLFTLAIGIGFMLCLVPGVIIAIACQFYALFIVDKQLS
                     GVEGLKASWEITKGHRGNLLVLSLVLAGVMLLGAAACCIGFLLIAAPMQVLAQSYVYL
                     KLHSEEPSPAA"
BASE COUNT         2038 a         3986 c         4078 g         2302 t
ORIGIN      
        1 tggctcgctc ggcgttcgag aagcccgcga gcgcctgctc ttccacccgc cgcatcgccc
       61 gcgggatctg cgcccgaagc cgcgccccag ccggggtcag atggatgacg acttggcgac
      121 catcgctcgg atgcttcttg cgagtcacga gccccgacgc cgccattctc tggagggcac
      181 ggacgacggt gggaccctcg accccgattg cctccgcgat ttctcctggc gtctgcccgt
      241 cgtgctccca gagccgctcg aggatgaagt tctgccccgc gtgaacgccg tggcgctcga
      301 gcggcacctg tatcgatgct ttcagtgctc gcgaggcgcg cgcgaccacg tcgagaaggg
      361 acggaacggc ctcttcttgc ggccggcggg tgttcattgg gagagtttta catcgtctgg
      421 gggacgactg tggtggctct gttcggctcg tcttcggggt gctctcgcgc aaccgtgaga
      481 ttaccgactc tggttgccga ccgcgcgcgc cgcgccgttc gcagtggata tgcggcgagc
      541 gtcagcgcct atgggctcgc gcgctcgaag agaagggcaa agtggacgag tggcgggggc
      601 atcggctcgt gacctcggat cccgcgaaca tccggcggct cgatcccgcg ctgccggtga
      661 ttaccgtcta ggtgcccgaa cgccgccgca tcgttcgggc acagccacgc gagggcgcgt
      721 ctcgggcaca cccaaaagcc gaaaccgcgg cgccccgctg cgatgcgaga ggccgcggtt
      781 ctgttcccgc tggtggtgcg tagcgcttac ttgagcagcg acttcaaagt gctgcccatc
      841 tccgcggcgc tcggggcgac tttgacgccg gcgctttcga gcgccttgat tggcgtcggt
      901 aggaggccgg tagcgttcca aggtgatggc agtatcgctg ctaggacgga tcctgtccgc
      961 tctatcgctg ctaggacgga tcctgtccgc tctgctgaac gtcgctgtcg tatgggtggc
     1021 tcatgatcga agcgttcatc cccgcgtctg ccactagagt cgtcgtctac ctccagcccg
     1081 tcaacgtgcg ctggggcgtg gacaagctcc gagtattctg ccgggacgag atgcgcatgg
     1141 agccgaacga cagcacctgc ttcctattcg tgaaccgcgc gcgagacact ttgctgctgt
     1201 accatctggg gtattccggt gagcagacct tgataaagaa gttggacaag ggttcgttcg
     1261 tgcttcccgc tcccgaagag aacggacgcc gattcgtaat tctacggcca gtgatgctgg
     1321 ggcgcttgtt ccgatcgtga cgtcgcctcg ggttatcgcg cacgccggca tcgctctcgg
     1381 gagctgagct atggcaagga aacatgggag cgatccccgc gcactcccgg ctcgcactcc
     1441 agggcgtttg ctgcgcctgg ggtgtcccaa cgcgcgcgac ggccgtcgac gtcagcgggg
     1501 cacgccggcc gaagccagcc gctcgcgcaa acgcgcgcgg acgggagcat cgtcgtcgag
     1561 cgcttcgtcc caaccgaaat cccggagaga cagcatcagt cgctcaatat cagcgagctt
     1621 ctcttcaatg gtaggcgctg gcgcgactcg tcgctgcaga tagcggctag cgccaactcg
     1681 ggcgccggtt caaccatcgg cgcgtagcca ctcggcgagg aggcgatcta tttcggcctc
     1741 ttcgacttcg ggaaactggc gacgcagagt tgagcgcttc attcggattc ccgtgcgcat
     1801 gacctcgagc gcttgctgca gcttctcgga gggcgtgagc tggcgagcgt gctcacgatc
     1861 atgggcaagg agcgcatcca ttcgcccaag tcgactcgag ccggagcacc cagtcatccc
     1921 gcaggtccac gccccggctg ccaagagcgt ttctacgcag cgggacggag cgccaggtgc
     1981 aggttacagg caagctcgcg gtacagcggt agtatcgccc ctcgattgga acctccgccg
     2041 ctccgcgtac caaaaagccg aaaccgcggc gccccgctgt gatgcgagag gccgcggctt
     2101 cgagtacctt tccggcgcga cgtaaacggc cgcgcgtgtg ttttacttga gcagcgactt
     2161 caaggtgctg cccatttcgg cggggctcgg agcgactttg acgccggcgt tttcgagcgc
     2221 cttgatcttg tcgctggcgg tgcctttgcc accgctgatg atggcgccgg cgtggcccat
     2281 gcgcttgccg gggggagcgg tggtgccggc gatgaaagcg gcgaccggct tttttacgtt
     2341 tttcttgatg tactcggcgg cgcgctcttc ggcgttgccg ccgatttcgc ccatcatcat
     2401 gatcgcgtgg gtgtcggggt cctcgttgaa gagggtgagc acgtcgacga agtccatgcc
     2461 gccgacgggg tcgccgccga tgccgatgca cgtggactga ccgaggccga gcgcggtgag
     2521 ctggcccacc gcttcgtacg tgagggtgcc ggagcgcgag acgacgccga tgttgccggc
     2581 gcggtggatg tggcccggca tgatgccgat tttgcaagcc tcgggggtga tgatgccggg
     2641 gcagttcggg ccgatgaggc gcacgggctg gccgccgacg gtcttgccgt cgatggcgcg
     2701 acgcacgcgg atcatgtcca tcaccggaat gccctcggtg atgcagacga cgagcttgca
     2761 gcccgcgtcg acggcttcga ggatcgcatc ggcggcgccg agggcgggca cgaagatgca
     2821 cgaggtgtcc gcaccggttt gcttcaccgc ctgggcgacg gtgtcgaaca ccgggacgga
     2881 gatcgctttg ccgccggggc cttcgctcgc gaacgtctca ccgccgcgac ccggggtgca
     2941 acccgcaacg atttgagtgc cgtacgcgat gcactgtttc gcgtggaacg agccggccga
     3001 gccggtgatg ccttgaacga cgacgcgcgt gttcttgttg accagaatcg ccatgaaact
     3061 ttgttcctct cacgcggcgc gggtggccga aacgatcttc ttggcgccgt cggccatgcc
     3121 gtcggcaggt tgaatcgcaa gcccgctgtc gcgcaggatc tgcttgccct tttcgacgtt
     3181 ggtgccttcg aggcgcacga cgagcggcac cttcaagccg agctcgcgcg tcgcggcgac
     3241 cacgccctcg gcgatgacgt cgcacttcat gatgccgccg aagatgttga cgaagatgcc
     3301 cttcaccttg gggctcgaca ggatcatctt gaaggccttg gtgacctgct ccttcgtcgc
     3361 gccgccgccg acgtcgagga agttcgccgg gctgccgccg tggtgctgga tgatgtccat
     3421 cgtggccatg gcgaggccgg cgccgttcac gaggcaaccg atgtcgccat cgagcgccac
     3481 gtagttgagg ccggcttcct tggcttcgag ctcgaccgga tcttcttcgt cgcggtccat
     3541 gagctcgttc cactcacggt gacggccgga ggcgttgtcg tcgaagttga tcttggagtc
     3601 gagcgcgacg atcttgccct gcttcgtgac gatgagcggg ttgatctcgc aaagcgagca
     3661 gtcttccttt tcgaagagct cgacgagctt ggtcagcgtc ttgatgaact cggcgaaggt
     3721 ctccttcggg ttcgggacgc catcggtgat cttgaggccg tagccgatct tgcgggcttg
     3781 gaagccgctc agaccgatga cgggatcgat gtgctcgatc aggatttttt cgggggtctc
     3841 ttcggcgacg cgctcgatct cgacgccgcc ttcggtcgat gccatcaccg cgatgcggcg
     3901 cgtctcgcga tcgacgacga ggccgaggta gagttcgcgc gcgatatcga gcccttgctc
     3961 gacgtacagg cgccgcacct tctgaccttc gggtccggtc tggtgcgtga cgagctgcat
     4021 gccgagcatc gcctcggccg ccttgcgcgc ctcggccggc ccgcccttga cgaccttgac
     4081 gccgcccgct ttgccacgcc caccagcgtg aatttgcgcc ttcaccacga ccaccgagcc
     4141 gccggtttcg gcgaggagct tggtggcggc cgcttcgacg tcaccgccgt tgaagcacgg
     4201 gatacccgac ggcacggcga tgccgtactt cgcgaagatt tgcttgccct gatactcgtg
     4261 aattttcatg agaaatatcc tgtcgatgcc gcggcgaaga tgcgctcgcg aacgcggccc
     4321 gaagcgagga tgccccgggc cgcgtcgccg tcagctcacg tggatgcctt gacgatgtcg
     4381 acgaccttct gaacgctgtc ggcgctcttc ttcaacatgg ccttttcggc gtcgttgagc
     4441 tcgagctcga tgatcttctc gacgccgccc gagccgatca cggcgggcac gcccatgtag
     4501 agatccttat agccgtactc accctcgagg tagacggccg acgcgagcac gcgcttctgg
     4561 tcgaggaggt aggcctcggc catggcgatc gcgctgatgg cgggcgcgta atacgcgctc
     4621 gtgcccatca agccgacgat ctcgccgccg cccttgcggg tgcggtcgac gatcttgtcg
     4681 agcgcctctt gcttcacgaa cttctggatc ggcacgccgt tcacggtgca gtggctgagc
     4741 accggcacca tgtcgtcacc gtggccgccg agcaccatcg tgcgaagatc tttcacgctg
     4801 cagcccgctt cgcgcgcgag aaagagcgag aagcgcgcgc tgtcgagcac gccggccatg
     4861 ccgacgatct tctggcgcga gccgccggtg cggcgcttgt actcgtagac catcgcgtcg
     4921 agcgggttcg agatgacgat cacgaacgcg tccgggcagt gcttcttcgc ggcgtcggcg
     4981 acgtcgcgga tgatcggcag gttggtcgcc acgaggtcgt cgcgcgactg gcccggcttg
     5041 cgcggaatgc ccgcggtgat gatgaggacg tcggaaccgg ccacgtcttc ccacttgctg
     5101 gtgccgacca cgcgcgagtc ggcgccggag atcgcgctgg cttgctcgag atcgagcgcc
     5161 ttgcccttgg cgaagttctc cttggcgggg atgtcgaaca gcacgacgtc gccgaggctc
     5221 ttcgtgacgg cgaggtttgc gagctcaccg ccgatgttgc cggcaccgat cagactgatt
     5281 ttctttcttg ccatggcttt tccttcgcct ctcacatgtg gtcgatgatc gcgcgaccaa
     5341 actccgaaca tttcacttct ttggcgcctt ccatcaggcg cgcaaagtcg taggtgaccg
     5401 tcttggcgcc gatggcgccg tccatgccct tgatgatgag atccgcggct tcgacccaac
     5461 ccatgtgccg gagcatcatc tcgccgctca ggatgaccga gccagggttc acgcggtcga
     5521 ggttcgcgta cttcggcgcg gtgccgtgag tcgcctcgaa cacggcgtgg ccggtgtcgt
     5581 agttgatgtt gccgccagga gcgatgccga tgccacccac ctgcgccgcg agcgcgtcgc
     5641 tcaggtagtc gccgttcagg ttcaacgtgg cgatcacgtc gaagtccttc gggcgcgtga
     5701 gcacctgctg cagcgtgatg tcggcgatcg agtccttaac gcgcaggagc ttcttccact
     5761 tgccgtcacc gtgcgtcgcc ttgagagcgg ccgccgcgtc gacctcggcg cggagctcgg
     5821 cctgagtttc tgggggcgcc atgtcgtagc ccggctcgat gagcttcgcg ttctcttcgg
     5881 cgctgaggct cggcttctct tcgaggttgc cgaggatcca agcttcgcgc tcggtgacga
     5941 tctgcgaacg gaactcgcgc ttcgcgacct cgtacccgaa gtcgcggaac gcgccctcgg
     6001 tgaacttcat gatgttgccc ttgtgcacca gggtgacgct cttgcgaccg aaggtgagcg
     6061 cgtattgaat cgcagcgcgc acgagccgct cggtgccttc tttggacacg ggcttgatgc
     6121 cgaagccgga cgtctccggg aagcggatct tcttcacgcc catttcggtc tggaggaagg
     6181 tgaggagctt cttcgcttcg ggcgttccgg aggcccactc gatgcccgcg tagatgtctt
     6241 cggtgttctc gcggaagatc accatgtcga cgtcgccggg gcgcttcacc gggctcggta
     6301 cgttggtgaa ccagcgcacg gggcgcaggc agacgtacaa gtcgagctgc tgacgaagtg
     6361 ccacgttcag cgaacggatg cccttgccca ccggcgtggt gagcggacct ttgatgccaa
     6421 cgaggtactt gcggtaggcc tcgaccgtct cgtcgggcaa ccagctgtcg aacttctttt
     6481 tgctcttctc gccggcgtag acttcgtacc aggcgatttt cttcttaccg ccgtacgctt
     6541 tttgcacgga cgcatcgagc acggcctgcg aggcccgcca gatgtcgggg ccagtgccgt
     6601 cgccttcgat gaaaggaatg atgggttggt cgggaacgct cagtttgccc gacgccaggg
     6661 tgatggcgtc gccactcttg ggaccttcga agagagccat attcggatgc ctcacacgca
     6721 cgcggccggc ttcgttgccg gccgtgagcg aagtagtcca gcgggctgtc cgtggcaact
     6781 tggcccgctg gtttccccgt gtttcggcca ctccagaaaa aaaaccgaca cccgcgtcgg
     6841 aaacgcggcg gactcacaac gagcgggcgg ccccgcggtg tatggtcctc ggcgtgtttc
     6901 gtacggatcg ggaccgcgct cgcttgctgc gcctgggact cgcggcgacg ctgtcttgct
     6961 gcgcttgtac gggagccccc ctaccgcgta gcgatgcggc ccagcgcgcc gcgggcgaac
     7021 ccaaccaaat caaggtcgac ccctgccgaa acgaagccgg caaaccaccg cccgccgcgc
     7081 tcgctcgtgc gtatcagggt ctgctcgcga ccgcgcgctg cgtggccgag gtcgacgtga
     7141 tgatgcagga agtgtcagcc gcgctcggcg tacgatgtga ctactgtcat gtgggcaccg
     7201 actatgcccg cgagaccgag aagagccgca tcgcgaactt catggccacc gagctcgtgc
     7261 ctcgactcga cgccaaggac cacaccgaag tcacctgcgt tcgctgtcac cgcggcgtcg
     7321 cgaagattct cggcgcacct cgctcggagc cgcgcgcgct cgagtggatg acggcggagc
     7381 tctcgccgaa cttcgtgacg aaggacgatc gaccgcttcg ctgcaagtcg tgtcatggcg
     7441 cggactgggg cacgcccggg tttcaccgca cggtgatctt gacgcacgag ctcgacgggc
     7501 taccgcgcgc agcggcgaag tcgccgtgac ggagcgcgcg cgtctgttcg tgtacggaag
     7561 cttgaagcgc ggcttcgcaa accaccgcgt gctccgaaac gcggatttcc tcggcgagtg
     7621 tcgcactgca ccccactacc ggctcttcga tctcggagcg tacccagcgc tggccacgga
     7681 tggtgcgctc tcgatcaccg gcgagctcta ctcgtatccg gtggagcact tctcggagtt
     7741 ggacgctttc gaaggtcccg cgtatcgacg tgggactgtc gcattgtcgg acggctcgac
     7801 cgcggaggcg tatttgctgg taactgcaga atgtgatgag gcgattgagc ttcacaccga
     7861 tacgtggcgc gcgcgctgat tcgaattttc gtggccttct tcaatggacc gctacttgct
     7921 ttggtgagca gcgtgacttc gccgatccaa tccatggtga tcccacgacc gatgcacgtc
     7981 gtcatcgtcg acgacgatcc ggaagtggtc acagggttac gtcgtgtact ccactctgcc
     8041 gaacccgaat ggctcatttc cacggcgaac aacgggaaag aggcgctctc cctgatgtcg
     8101 ggcgagaagg tcgacgtggt gatcaccgac ctacacatgc cggagatgga tggcttccag
     8161 ctcctcggac agctcgaacg cgctcatccc gagacgatcc gcatcgtcca ctcgtctcat
     8221 actgcgacac tcgccacgca gttgatgcgg tacctggcgc acaacgtgct cacgaagccg
     8281 acacccgcgc gagatatcgt cgccatggct cgctgggccg tgcatgcggc gggagcggtg
     8341 cggcgctacg aagcttgcga agcctagcgc gcagcgtgtt cgccctcact gcgccttcgg
     8401 agccaaaccc ggtaccactt cggcgaggta cttcttcgtg agcttcgcga acgacttgtc
     8461 ggccatgtcg tcgtagatct cttcgaaggg gcgcccctcc atcatgctct ttacgtccgg
     8521 cgagttccac gccgagtact tgaagctgtg aaccgagcct gccccaggca agagcatgct
     8581 gatgcggaac agcacgccga cgccagttat tgcagcgcgt gggttcttca tcaagtaagc
     8641 gcccgacatc tcggtgcggt ggatgatgag gatggtcggg acggtgacct tcggatcttc
     8701 gtcggtgttg tcgtcgtagg cgggccctga ctcgaacttg agaacgtctt cgtcgaaggc
     8761 ctgagagagc gcttcgatga tctgcttctg cgtgggccgc tcgcgctgcg cggcgtgcgc
     8821 ggcgtcgaag tactgccccg gcaacgacgc cttgccaccg taccaatggg ccttctgcag
     8881 tagctcctcc gatttcgcga ccgagtcggg taggcggcgc ttggagcgca cttgaacgac
     8941 gccgtcgtgc tcgacgccgt acgcgaccag tcggcgaaac gcgtcgagca cctcgggctt
     9001 gggatgagcc ttttcgacga agcgagcgag cgcggcctcg gtgcgcgcgt tgatggcgcg
     9061 cgtgacctct cccaagatcg cgggctggtc ggcgaagcgc gcccggaact ccttcagtgc
     9121 gcgcagcgag ccggcacgct tcgcgtcctc gagcgcggcg agcagctgct tcaagagcgc
     9181 gctctgcgct tcgacgtcca cgggctcgcc ggcgtgggcc ttcacgaact gcacgagctc
     9241 gtcgatcgac tgcttcttca cgacctccgc gagctgggca cgcggcaaga gcagggtcgc
     9301 gacgtcgaga ttgctccccc cacgctcgag atacgcctcg tagccggcgc gcgtgtcgac
     9361 cttacgggcg accgcgtaca gagctcgctc acccaggctg ttgcgcaccg tgaagaccaa
     9421 cgcgccaaac ccagcgagca ctatcgccgc gagcggcacg gccacccacg aaagatccga
     9481 cttcggcttc ttgatgcgct cacggggcga gaatggattc gagaagccgt tgtccgcgag
     9541 cggatcccac accgcgcgtt cgcgcgcgct gaccggcgcg gcgtcgggca tgaagtattc
     9601 tcggaagccg ttcacggcgc gcgcgagctc gagggcgcgg ttcgcgtcgg tggtgtcgaa
     9661 ctgaaagcgc gctccgtcgc tgaacgcaag gttcaacttg cgcccagacg cggtcgcttc
     9721 gacgaggtcg cgcagcgaat gcacttccat gttgagcgag cgcgcgtcga cgactccggc
     9781 ggggaaggcg taaaccgcgg gcgcgatcgg ccagcggctg cgagcgcgtt ggatgcggaa
     9841 cgcgacgccg agcagcgcgc cacacgcgac gccgagcacg acgtacagga cgagcgctcc
     9901 cgtaggttgg agagcgaagg agctgtcgag ctttgcgtac ccgactcgcg tgagggccag
     9961 catcgtcacg agcgcgagcg cagccaatgc gagacagatg acgccgacgc gcggcatcaa
    10021 ctgtcgcttg atggcgagcg gtatcggcgg cgccgtccca cgggcggcgg ctacgaagcg
    10081 ctcttggatg ggtcgttcca gtccgaaaaa atcgacacgc tgcatgctgg cgacgccatc
    10141 tgaccggtcc aaacgagagg acttgcgccc gtcgctttgg gcaggtggcc tcgaaacctt
    10201 agaacaagtc gcgctgcaac acacgaggcc gttgtgacgg tcttcgtttc cgtcgatttc
    10261 tcggaggatc cccaagggaa ttgcgccacc gccccgcccc gggcggggta tgcaagggcc
    10321 cacatggacg ctctctacga ctttatcgtg atcggtgggg gctcgggcgg cctcgccgcg
    10381 gcgaggcggg cacgtcgtta cggagctcgg acggtcgtcg tcgagccgca tccgctcggc
    10441 gggacctgcg tcaaccgcgg ctgcgtcccg aagaaaattc tatggaacgc gtccgagctc
    10501 gccgacaagc tcgtcgatct gccggactat gggttcgaac cgcgaccacg gggtgccttc
    10561 gactacgccc gccttcgcga ggcgagtcga gcgtacgtct cgcgcatgaa cgcgaacttc
    10621 gcggtgcggc tcgacgaaga gggggtcgag ctcgtgcgtg cgacggctcg cttcctcgag
    10681 cccacggtcg tcgagacctc tgacggacga gtcctccgcg caccgcacgt gctggtggcg
    10741 acgggttcgc gtcccgcctg gcctcgagtc cccggcgccg agctcggcat cagctcggac
    10801 gattggttcg ggttggatca gctgccgcga cgcttgctgg tcgtaggcgg cggttacatc
    10861 ggcgtcgagc tcgcaggcat cgcgcattcg ctcggcagcg acgtcgtgct gacgttccgc
    10921 ggagaggcgc cgctcgctcg cttcgacgac atgctgcgca ccacgctcgc cgacgagatg
    10981 cagcgcgtcg gtatcacaca ggtcgcgggg ttcaggccag cgagggtgtc gcgctcgggg
    11041 ccagggggat tgcaggtcga aggagaagac gggcgcttgc tcgaagactt cgattgcgtg
    11101 ctctgggcaa ctggacgagt gcccaacgtc gagtccttgg cgctcgagaa ggccggcgtc
    11161 gcgctcaacg ccgcgttcgc gatcggcacg gacgcttttc agaatacgtc gatgccaggc
    11221 gtctacgcgg tgggcgacgt gaccggggtg tccatgctca cgccggtcgc gatcaccgcc
    11281 ggccgaaagc ttgccgatcg cttgttcggc gggcaacccg acgcgaggct cgattacgaa
    11341 gacattccga ccgtggtctt cagccatcca cccatcggaa cggtcgggtt gagtgaggcc
    11401 atggcgcgtg ctcgctacgg cgaccaggta aagacgtacg tcacgcgctt caccggcatg
    11461 tactacggga ttactcagcg caagccgcgc accgccatga agctcgtcac gttgttaccg
    11521 gacgagcgcg tactcggtat tcacgcgata ggcctcggcg ccgacgagct gatacaaggc
    11581 ttcgcggtcg cgctgcgcat gggggcgcgt aagtcggatc tggatcggac agtcgcgatt
    11641 cacccgaccg ccgccgaaga gctcgtcacg atgcgctgac cgagctatcg gcggcaggaa
    11701 gctgcgaagg atctacgcgg cggggctcgg ctcctcggag tgtagcttca ggtacacgta
    11761 cgactgcgcg agcacctgca ttggcgcggc gatgaggagg aagccgatgc agcacgccgc
    11821 cgcaccgagc agcatgacgc ctgccaacac cagcgagagc accaggaggt tgccacgatg
    11881 gcccttcgtg atttcccagc tcgccttcag accttcgaca cccgagagct gcttgtcgac
    11941 gatgaagagc gcgtagaact ggcacgcgat cgcgatgatg acgccaggta ccaggcagag
    12001 catgaagccg atgccgatgg cgagcgtgaa cagcagctgc cccacgaaca tcgcgccgaa
    12061 ggtcgagcca cccgcgaaca cctctccgaa ctcgggcttc cgcccgcgcg cgaccgtgag
    12121 cgcaaaccgc gtgataccgc ccaggaagta cgcctgggag agcacgccca cggctgcgga
    12181 tatgggcgtc atgatgaggc cgatgagcgt cgccgtgtcc gcgtcgatcg ctcccgacgt
    12241 cagcaagaag ctctgagcaa agccgcgcgc gccgcccaac aagctctgcg gtagcaacat
    12301 cacgaaccag ccgacggcca gcggcaatgc gaccccgccg aaatccttcg tcacccaaga
    12361 ccaggcgaac gagaccgcgt ccatcgcttg ccacggaccc gagc
//
LOCUS       DASTKS010000078         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_233569, whole genome shotgun sequence.
ACCESSION   DASTKS010000078 DASTKS010000000
VERSION     DASTKS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233569"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFQ35_01680"
     CDS             complement(<1..389)
                     /locus_tag="VFQ35_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929360.1"
                     /translation="MAAPSLNFPAPETTDQKAVASALEAGGVEWRRGDFREAVRWLHR
                     AADAAQASGDERRGVGLARVAAQLMAQLEMPPESSPRDDVSALASFDDFNDQTIVDAP
                     VLTTRTAQQSGVGLVDSEPAAAPKPEPP"
     gene            555..1997
                     /locus_tag="VFQ35_01685"
     CDS             555..1997
                     /locus_tag="VFQ35_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET9929361.1"
                     /translation="MNATSPEVEQRPVPLTAFERRFGHPLGLVVLFFTEMWERFSYYG
                     MRGLLKLYMANYLFVTAREALQGCRGHTPPCALVPGDPNTVLFWSSLQHLLPSNPTEA
                     ASILYGAYTGLVYFTPFIGGIIADAVLGQRKTVVIGALLMAAGHFVMALENSFFIALS
                     LLIIGNGAFKPNVSTQVGSLYPKGDPRRDRAYTIFYMGINLGAFICNLICGTLAALYG
                     WHYGFAAAGFGMLLGLVVYLAGQKHLATDLLTREKREHKAHEPLSKDDMRRTIALVVV
                     CALTIVFWAVYEQQGNTMQSWADEHTRWPTVFGFQIPSTWFQSWNPLLIIMLAPLLDM
                     FWAWQSRARKEPSTLSKMSLACFILGSSFIVMIVGAELVGDGKGSLFWPLFCTFLLTI
                     GELYLSPIGLSLVSKVAPARIVSLMMGLWFFSSFLGNFLSGYIGVFYTRWSKEAFFLL
                     LTGLGFAAGLAMLTLKKPMSAAIEQRGTSP"
     gene            2055..>2693
                     /locus_tag="VFQ35_01690"
     CDS             2055..>2693
                     /locus_tag="VFQ35_01690"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9929362.1"
                     /translation="MRIRRLARVALGSCTFVLACSGSSSSGGEGGATTSPHGGANNAA
                     GGATGGNDLARGGDVSRGGSGDGGANSSGAGFGGRVNGTGGSTTLSAGGKTSGTGGTS
                     ARGGNASTGGMSAGGASTGGTSAGGKSSGAGGSMGGAANTACKRGIAANTAPGAAFSN
                     AISWWYNWAMTGTGAGSGIEFTPMAWGSGSVKTALPSGAKYLLGFNEPNFHAQ"
BASE COUNT          431 a          889 c          883 g          490 t
ORIGIN      
        1 ggcggctccg gcttcggcgc ggccgcgggc tcgctgtcga cgagacctac cccgctctgc
       61 tgcgccgtcc gcgtcgtgag caccggcgca tcgacgatgg tctgatcgtt gaaatcgtcg
      121 aaggaggcga gcgccgaaac gtcgtcacgc ggagacgatt cgggcggcat ctcgagttga
      181 gccatcagtt gagccgccac ccgagcgagg ccgaccccgc gacgctcgtc acccgacgcc
      241 tgagcagcgt ccgccgctcg gtgcagccag cgcacggctt cgcggaagtc cccgcggcgc
      301 cattcgactc cgccggcctc gagggcgctc gccaccgcct tctggtcggt ggtctccggt
      361 gcgggaaagt tcaggctagg agctgccata gaggacgcga tcttagccgc ctggtgccat
      421 cacgaaaggg ggttctctgc gcacttttcc ccacgtcgcg cgcccgcctc gtccaatttc
      481 gctcgtgagt tcccccgttt acgggctcgg gtgctctggc cgctcagccg agaggcggtg
      541 taacgtcgcc cgcgatgaac gcaacctccc ccgaggtcga gcagcgcccg gtccctctga
      601 cggctttcga acgtcgcttc ggacaccccc tcggactcgt cgtcctgttc ttcaccgaga
      661 tgtgggagcg cttcagctac tacggcatgc ggggcctgct gaagctctac atggcgaact
      721 acctgttcgt cacggcgaga gaagcgctgc agggatgtcg aggacacact cccccttgcg
      781 cgctcgtccc gggcgacccg aacacggtgc ttttctggag ctcgctgcaa catctgttgc
      841 cgtcgaaccc gaccgaagcc gcgtccatcc tctacggcgc gtacaccggc ctcgtctact
      901 tcacgccctt catcggcggg atcatcgcgg atgcggtgct cggacagcgt aagacggtgg
      961 tcatcggggc gctgttgatg gccgccggtc acttcgtgat ggcgctcgag aactcgttct
     1021 tcatcgcgct cagcctgctg atcatcggca acggtgcctt caagccgaac gtctcgacgc
     1081 aggtgggcag cctgtacccc aagggcgatc cgcggcgcga ccgcgcttac acgatcttct
     1141 acatgggcat caatttgggc gccttcatct gcaacttgat ttgcggcacc ctcgcggcgc
     1201 tctacggctg gcactacggc ttcgctgccg ccggcttcgg catgctcctc ggcctcgtcg
     1261 tgtacctcgc agggcagaag cacctggcga cggatctttt gacccgcgag aagcgagaac
     1321 acaaggcgca cgagccgctg tccaaagacg acatgcgaag gacgatcgcg ctcgtcgtgg
     1381 tgtgcgcgct cacgatcgtg ttttgggctg tgtacgagca acagggcaac acgatgcagt
     1441 cttgggccga cgagcacacc cgctggccaa ccgtgtttgg ctttcaaatc ccgtcgacct
     1501 ggttccagtc gtggaatccc ctgctcatca tcatgctcgc accgctgctc gacatgtttt
     1561 gggcctggca aagccgcgca agaaaagagc cgtccacgct ttcgaagatg tcgctcgctt
     1621 gcttcatcct cggctcgagc ttcatcgtga tgatcgtcgg ggccgagctc gtgggggacg
     1681 gcaaaggcag cctgttctgg ccgctgttct gcactttcct cctcaccatc ggcgaactct
     1741 acctctcccc gattgggctt tcgctcgtga gcaaggtggc gcccgcgcgc atcgtttcgt
     1801 tgatgatggg cctctggttc ttctcctcgt tcctcgggaa cttcttgagc ggctacatcg
     1861 gagtcttcta cacgcgctgg tccaaagaag cgttcttcct cttgctcacc gggctcgggt
     1921 tcgcggcagg ccttgccatg ctcacactga agaagccaat gagcgcggcg atcgagcagc
     1981 gcggcacatc tccgtgattg tcccacacga ggaacgggcg aggtcacggc tcgcgtcgtg
     2041 ctaagctcac gcgaatgcgc attcggcgac tcgcgcgggt agcgctgggt tcatgtacgt
     2101 tcgtcctcgc ctgctcgggg tcgagctcga gtggtgggga aggcggcgcg acgacgagcc
     2161 cgcacggcgg agcgaacaac gccgcaggcg gcgcgaccgg aggcaacgac ttagcgcgcg
     2221 gcggcgacgt atctcgcggc ggtagcggcg atggcggcgc caattcgagc ggcgcgggct
     2281 tcgggggccg ggtgaacggc accggcggga gcacgacgct cagcgctggc ggcaaaacca
     2341 gtggcaccgg cggcacctcg gcgcgcggag gtaacgcgag caccggtggc atgagcgcag
     2401 gcggcgcgag caccggtggc acgagcgcag gcggcaagtc gagcggcgct ggcggttcga
     2461 tgggcggcgc cgcgaacacg gcgtgcaagc gcggcatcgc cgcgaatacc gctcccgggg
     2521 ccgcgttctc gaacgcgatc tcgtggtggt acaactgggc catgacgggc accggtgcgg
     2581 ggagcggcat cgagttcacg ccgatggctt gggggagcgg tagcgtgaag acggcgctcc
     2641 cgagcggcgc caagtacttg ctcggcttca acgaaccgaa cttccacgcg cag
//
LOCUS       DASTKS010000079         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_458154, whole genome shotgun sequence.
ACCESSION   DASTKS010000079 DASTKS010000000
VERSION     DASTKS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458154"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..700)
                     /locus_tag="VFQ35_01695"
     CDS             complement(59..700)
                     /locus_tag="VFQ35_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929363.1"
                     /translation="MRQSSMLVVVPLLATACPREQRAPSDRSCASACAGLVEVECPLA
                     AHQSCLRACDAERASAAQARCGVEYEAFLSCLSRAKAACPDSPAESVRLGAGLSSCQR
                     QYTSYSRCARACREHGVVRTGARLLEVDERQVSVQAELVTSGCGRALPLEPRGAPPGS
                     RCEYQSVCAPVTCSCPSATEGYRARVCVDGACAGAESACRLAPRAVGHQVCRP"
     gene            complement(704..2074)
                     /locus_tag="VFQ35_01700"
     CDS             complement(704..2074)
                     /locus_tag="VFQ35_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9929364.1"
                     /translation="MQEFGGAAGANRGGSLPGFVLLYAEPFSLLPAAFPFTRQRLVIG
                     REPPADLVLPVSAVSRVHAEVNWAEGRFVIRDLGSRNGVLVNGRRVREDALEAFDEVR
                     VGDALFKFVEREAEEYARYRIDGSMVPGARRYAQHPTSLVGGYQMDRIVADLERIAAA
                     ELNVMLLGPSGTGKEVVAGELHRLSRRPGPFCAVNCAAIPANLLESELFGYKRGAFSG
                     AERDKPGIIRAAHQGTLLLDEIGDMPLDAQAKLLRVIQSKEVLPLGATQAERIDVRFV
                     SATHRDLRRLQQENRFREDLFARLNEYQLRLPPLHERKEDVYALTRAFLNRLNRPELG
                     LSVPLMVALLHHDFPYNVRELEAAIKRAVALARTTLLDVELLPDAIRESLVEYGRPAE
                     GDASRADPANAHSDAGQAPGEDELRALLAAHAGNVAAVGRVLGKARMQVHRWMKRYNI
                     EVDDYR"
     gene            2247..3083
                     /locus_tag="VFQ35_01705"
     CDS             2247..3083
                     /locus_tag="VFQ35_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9929365.1"
                     /translation="MSNSENPVSPVRVAALQLSSQESVSQNLERVDALVTRARQGGAQ
                     LVVLPENFAYMGDEAGKRAIAERIGDREAPIQSALRRIAQRERVALVAGGFPERSSDP
                     ERPYNTSACIDPAGELVATYRKIHLFDVDLRSHGTLCESANTTPGKEIVVAPLLGLSV
                     GLSICYDVRFPELYRALVDHGADLITVPAAFTLYTGKDHWHPLLRARAIESQCYVVAA
                     AQWGKHPGNRATFGHALIVDPWGTVVAEASDTIGIASADVDLAFLREVRARVPSLAHR
                     RL"
     gene            complement(3241..>3625)
                     /locus_tag="VFQ35_01710"
     CDS             complement(3241..>3625)
                     /locus_tag="VFQ35_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929366.1"
                     /translation="AEQLYRRAVELDPWLTIAYTNLGNIRFRRQDTEGAEQLYRKALE
                     IDARQPEAQYNLGYVMLERGQPELSVPLFLGAIAADPKFSDAYFNLAMAYEQIGETQK
                     ARPYWKSYISLEPSGTWTEIAKRHL"
BASE COUNT          599 a         1264 c         1173 g          589 t
ORIGIN      
        1 ccccccggtt tctcggccgt tttggccttg gcctccagag gcgaggagag tctccgattc
       61 acggccgaca gacctgatgg ccaacggctc gtggggccag ccggcaagca ctttcggcgc
      121 cggcgcaggc gccatcgaca caaacgcgag ctcgatagcc ttcagtcgcc gaggggcacg
      181 agcacgtcac cggagcgcac acgctctggt attcgcagcg cgatccgggc ggagcgccgc
      241 gcggctccag cggaagcgcc cgtccacagc cgctcgtgac gagctcggcc tggacgctga
      301 cttggcgctc atccacctcg agcaggcgcg ctccggtgcg aaccacgcca tgctcgcggc
      361 aggcccgcgc gcagcggctg taagacgtgt attgccgttg acagctcgaa aggccagcac
      421 caaggcgtac gctctccgcc ggcgaatccg ggcacgcggc cttcgcacgg ctcaggcaag
      481 agaggaaagc ctcgtactcg acaccgcagc gcgcttgggc ggcgctcgcg cgctcggcgt
      541 cgcacgcacg aaggcagctt tggtgagcgg cgagggggca ctccacttcg acgagcccag
      601 cgcacgcgga cgcacacgac cgatcgctcg gcgcgcgctg ttcgcgcgga cacgccgtcg
      661 cgagcaacgg aacgacgacg agcatcgagc tctgccgcat ggctcagcgg tagtcgtcga
      721 cttcgatgtt gtagcgcttc atccagcggt gcacttgcat gcgcgccttg ccgagcacac
      781 gcccgacggc ggcgacgttg ccggcgtggg cggcgagcag cgcgcggagc tcgtcttcgc
      841 ccggggcctg acccgcgtcg gagtgcgcgt tggccgggtc cgcgcgcgaa gcgtcgcctt
      901 cagctggacg accatactcg acgagcgact cgcggatcgc gtcgggcaag agctccacgt
      961 cgagcagcgt ggtgcgcgcg agcgcgacgg ctcgcttgat ggctgcctcg agctcccgca
     1021 cgttgtacgg aaagtcgtgg tgcaagagcg ccaccatcaa cggcacgctg agccccagct
     1081 ccgggcggtt cagccggttc aaaaacgcgc gggtgagggc gtaaacgtct tctttgcgct
     1141 cgtgcagcgg cggaagccgc aattgatact cgttcaggcg cgcgaacaga tcttcgcgaa
     1201 agcgattctc ttgttgcagc cgccgcaaat cgcgatgcgt ggccgacacg aagcgcacgt
     1261 cgatgcgctc ggcttgggtc gcgccgaggg gcaggacctc tttgctctga atcactcgca
     1321 agagcttggc ttgcgcgtcg agcggcatgt cgccgatctc gtcgagcaag agcgtgcctt
     1381 ggtgtgcggc gcgaatgatg cccggtttgt cgcgctcggc gccggagaac gcgccgcgct
     1441 tgtagccgaa cagctcgctc tcgagcaggt tcgccggaat cgccgcgcag ttcacggcgc
     1501 agaatggccc cgggcgtcga ctcagccgat gcaactcgcc ggccaccacc tctttgccgg
     1561 ttccgctcgg tccgagcagc atcacgttga gctcggcggc ggcaatgcgc tcgagatccg
     1621 cgacgatgcg gtccatttgg taaccgccga cgagggaggt cgggtgttga gcgtagcggc
     1681 gcgcacccgg caccatgctc ccatcgatgc gatagcgcgc atactcctcg gcctctcgct
     1741 cgacgaactt gaagagcgca tcgcccacgc gcacctcgtc gaaggcctcg agcgcgtctt
     1801 cacgcacgcg gcgcccgttc acgagcacgc cgtttcgact cccgaggtcc cggatcacga
     1861 atcgcccctc ggcccagtta acttcggcgt gcacgcgcga gaccgcgctc actggcagca
     1921 ccaaatccgc gggcggctca cgaccgatga ccaagcgttg gcgagtgaac ggaaaggcgg
     1981 ccggaagcag gctgaacggc tcggcgtaga gcaacacgaa gccaggcaag ctaccgccgc
     2041 ggttcgcgcc cgccgcgccg ccgaactcct gcatggcgcc tgtagtcata ctcacgcccg
     2101 aagcttagcg tgcggcgggc caatccgcgg caattttccg cgacttgcga cgccggctcg
     2161 atgtatcgcc gcatctggat cgagcggccc catcacggcg agtgcatctg agggcggggg
     2221 tcgcgtgaat ccgctataaa cagcggatgt ccaactcgga aaatcctgtc tctccggttc
     2281 gcgtcgcggc actccagctc tcgagccaag agtccgtttc gcagaacctc gagcgagtgg
     2341 acgccctcgt cacgcgcgcg cgacaaggcg gggcccagct agtcgtcctt cccgagaact
     2401 tcgcgtacat gggcgacgaa gccggcaagc gcgccatcgc cgagcgcatc ggggatcgcg
     2461 aagcaccgat ccagtccgcg ttacgccgca tcgcccaacg cgagcgagtc gcgctcgtcg
     2521 cgggcggctt ccccgagcgc agcagcgacc cggagcgccc gtacaacacg agtgcctgca
     2581 tcgatcccgc gggcgagctc gtcgcgacct atcggaagat tcacctgttc gacgtcgacc
     2641 tccggagcca cgggaccctg tgcgaatccg cgaacacgac gccgggcaag gagatcgtcg
     2701 ttgccccgct gctcgggctc tccgtcggtc tctcgatttg ctacgacgtg cgctttcccg
     2761 agctctaccg cgcgctcgtc gaccacggcg ccgacctcat caccgtgccc gctgccttca
     2821 cgctctacac cgggaaagac cactggcacc cgttgctccg cgcgcgcgcc atcgaatcgc
     2881 agtgctacgt cgtcgcggcc gcgcaatggg gcaagcaccc tggcaaccgc gcgaccttcg
     2941 ggcacgcgct catcgtcgat ccctggggca ccgtcgtcgc cgaagcctcc gacaccatcg
     3001 gcatcgccag cgccgacgtc gatcttgcgt tccttcgcga ggtccgcgct cgagtaccat
     3061 cgctcgcgca tcgaaggctg tagggctccc ttaattttcg gggtcgcctc gccgcagccc
     3121 atttgcgaag cgcacgtgcg tcgtcgggtt ccaccgagcg gcacgcacgt cagcgcgcag
     3181 cgctacgcat tcgccgaacc atccttgcgc ccccaaaccc agatcggtcg gaggcagggc
     3241 tcagaggtgt cgcttcgcga tctcggtcca cgtcccgctc ggctcgagcg agatgtagct
     3301 cttccagtac ggtcgggcct tttgagtttc gccgatctgc tcgtaggcca tcgcgaggtt
     3361 gaagtacgca tccgaaaact tgggatccgc ggcgatggct ccgaggaaga gcggcacact
     3421 gagctccggc tgcccgcgct cgagcatcac gtagccgagg ttgtattgcg cctcgggctg
     3481 acgcgcgtcg atctcgagcg cctttcggta aagctgctcg gctccctccg tgtcctgtcg
     3541 acggaagcgg atgttgccga ggttcgtgta ggcgatggtc aaccaagggt cgagctccac
     3601 cgcccggcgg tagagctgct ccgcc
//
LOCUS       DASTKS010000080         6125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_904165, whole genome shotgun sequence.
ACCESSION   DASTKS010000080 DASTKS010000000
VERSION     DASTKS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904165"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFQ35_01715"
     CDS             <1..753
                     /locus_tag="VFQ35_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9929367.1"
                     /translation="FKGGLLDPDLRRRPAQQLYNWCVRRVDNIQCVSEYTRDVLRAAG
                     YREESLLMIPNAVDLTRFSPRPRPERGTGEAARVAYVGRLRQVKGVSVLMEAWPHVAR
                     SSAARLTIAGDGPEREGLERAARESGVWESVAFAGEVSDVPAVLAEADIYVQPSFQEG
                     LPNSVLEAMAMGLPIVATRVSGNEDVVVDGENGLLVPPGDPKSLAAAIQKLVDDPALA
                     TRMGRRSREIIESRFSLAAVMRQLCDAYRGRA"
     gene            1490..2554
                     /locus_tag="VFQ35_01720"
     CDS             1490..2554
                     /locus_tag="VFQ35_01720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9929368.1"
                     /translation="MSFDAFPLRGESDRSAVLQLWRDNFGDQRIKTKIDPRWHWLTEA
                     NPSGPAKICVVTKRETGEVIGSAGAMPRVLRIGGSPVLAAVLCDFVVDKRHRVAGAAL
                     ALQRKLADACFADGISVLYGFPNKNAFPVFARIGYKTVGAATMMARPLRAGPHLRDHV
                     PALLAETAGFFVDCVLQAYDLQLYLRRARNLDDTQRPAADDSFQTLWDEVKVARAIVA
                     ERTPAHLNWRYAKHPVDDCTFFCLTEKSTGRLRGYVAYTVASGKVNVLDAFWASPEVQ
                     KALFVRFVWRMRALGHASISVCYAGDASVAATLRELGFIQSKESRNFVCKVAPHELDR
                     VGPAAYDLSQWSLFDGELDI"
     gene            2599..3402
                     /locus_tag="VFQ35_01725"
     CDS             2599..3402
                     /locus_tag="VFQ35_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929369.1"
                     /translation="MLALLRKVASLAGVGLTALGCASPVPEEQTSLARSPGVDPGKPS
                     RPISMVWLHHSTGDRLLRGGLFDALKADGIQFHDINYKEAVVDGFVIGDHTDVDDWPK
                     TFNTPKFFDTVKRWELGADKQAHDIVMFKSCYPNSDIKTDAQLEDYKRYFDSLVPTFQ
                     ANPDILFIAMSTPPLVKRNTSVAAASRAREWARWLTSEYSTRLRNVKVFDLFDALAIA
                     EGNPDANTLVPQFASGRGDSHPSPEGARAVTRLFIPWLNRTAREAGLSD"
     gene            3424..3684
                     /locus_tag="VFQ35_01730"
     CDS             3424..3684
                     /locus_tag="VFQ35_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9929370.1"
                     /translation="MPAPNIESRIREFVASNFGFRGASLNMDGNLDLIQQGILDSTGV
                     LEVVAFLENDLGVKVEDDEMIPENLGTMSRMISFVSRKKGSS"
     gene            3698..5242
                     /locus_tag="VFQ35_01735"
     CDS             3698..5242
                     /locus_tag="VFQ35_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET9929371.1"
                     /translation="MIRDSRSDEFLIDNRLRVHAARKPDHVALRIGERSFSYAQLNEA
                     ADSWAKLLRGLGVARGDRVLIALDNVYETVLGFYGALRADAVPSIVGAGRRFKRLERI
                     IALAEPRVLLTNSKVAQGLGLGVTRSCRVVLVDDVELEGAISLAAAMRECGSEAAPPA
                     RQSIELDLATLCWTTGSTGEPKGVMLTYQNLRNSASAIASYLEHGDDDVVMCVLPLLH
                     TYGLFQLLVTHFTGGTLVLEAGFSLAVPILKRLQEMRVTGFAGVPTIYASILALKDLD
                     RFDLSRLRYLTNAGYALPAAHLRRLQELCPETRIYAMYGQTECTRVCYLPPEQALERP
                     ASVGISMPNQELWLVREDGTRAEKGEIGELVVRGPNVMRGYWRDPEGTARALRPGRLP
                     GEVVLYTGDLFRTDEEGYLYFVARADDIIKTRGEKVVPCDVEQAISSLSDVVEVAVLG
                     IPDPIHGVAVKAVVVKRPGSSLTTDDVKRVVFTSIDEVAVPRVVEFVEALPRSEGGKV
                     MKSSLL"
     gene            5242..>6125
                     /gene="asnB"
                     /locus_tag="VFQ35_01740"
     CDS             5242..>6125
                     /gene="asnB"
                     /locus_tag="VFQ35_01740"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9929372.1"
                     /translation="MCGVAGLLNLGTSQPPPSRELALRMSSMLHHRGPDGYGIYRDRH
                     AVLAHRRLSIIDLEGGWQPMTDTDEQVYICANNEIFNYIELRQELEAKGRRFHSHCDT
                     EVIVQAYLEYGAEVAQHLNGQWAFALWDTRTQELVLSRDRAGILPLFWTVHGNVLRFA
                     SEVKALFADPEVPREFSPEGLDEALTFWAAVAPLTPYRGVQQLPPGVTAIFKAGSNVP
                     KIVDRVWPMLSGPARRASENAPQEQRVEAVRESLEQAAQLRLRADVPVGAYLSGGLDS
                     TILTSLIAAHRDVPLRTFS"
BASE COUNT         1053 a         1950 c         1990 g         1132 t
ORIGIN      
        1 ttcaaaggcg gcctcttgga tcccgactta cggcgtcggc ccgcgcagca actctataac
       61 tggtgcgttc ggcgtgtgga caacatccaa tgcgtcagcg agtacacccg cgacgtcctg
      121 cgagcggcag ggtatcgcga agagtcgctt ctcatgatcc ccaacgcggt ggatctcacg
      181 cgattttctc cgaggccgcg gcccgagcgc ggcacgggtg aggcggctcg cgtagcgtac
      241 gtgggtcgac tacggcaggt gaaaggcgtt tcggtcttga tggaagcgtg gccccacgtc
      301 gcgcggtcga gcgccgctcg cctgaccatc gctggcgatg gccccgagcg cgagggcctc
      361 gagcgcgccg ctcgcgagag tggggtctgg gagtcggtgg cgttcgcggg ggaggtcagc
      421 gacgtacccg ccgtattagc ggaagcggac atttacgttc agccttcgtt tcaagaaggc
      481 ctcccgaact ccgtgctcga agcgatggcg atgggtctac cgatcgtcgc tacccgtgtg
      541 agtgggaatg aagacgtggt cgtagacgga gaaaacggcc tgctcgtgcc gccaggcgac
      601 cccaagtcgt tggcggcggc catccaaaag ctcgtcgatg acccggcgct cgcgacgcgc
      661 atgggcaggc gctctcgcga aatcatcgag tcgcgcttta gcctggcggc ggtgatgcgg
      721 cagctgtgcg acgcgtaccg cggccgtgcc tgacgcgctt cctcgcggcg ttgcggtcgc
      781 acctcgcccg aacgagccgt cattcgtcgc ctgaatccac gctcggcgca cgtggtcgcc
      841 cggacgttcc ggtcctccgg gcggttggcg ctgcgcagcg gagatgacgt aaagcagctc
      901 gtcttcggcc cgcgcagtac gactcagcca ttagcggtca ttagagatag cggctctctc
      961 tgccagcacc ccgaaccgcc gggcccacgt gtggttccgc gacctgatga atgctgcgac
     1021 ttcggcaggt gaaccggtgc tcacgcgaac gcgccgcctc gtcccgtttg cgactgactc
     1081 agccactcgc cggcgccaag ttacgattcc gcgatttgct gagatttccg ggttcggagg
     1141 gccgggcaca ttgccactct gaatatcgag cgcgggtgaa accagaccgc gcgctgaacg
     1201 cgcaaatctt gcggggaatc gcgccgcggc gcagcgcggt cacatcgtcg caccatcaaa
     1261 acaaatgccc attcactcgc tgcacgcgaa gcgtcatttt ggggaaggta gttcgcgaac
     1321 gtatatgtcc cccatgtatc gttccggagc gcgttggccg ccgcgttcga ctcgcgcctt
     1381 cgatctccga gcgacgcgtt cttggcaaca cacataacgg tggcttccgg aacgtggtaa
     1441 aagagaaatg cgggtcttgt gtcatgcacc gtgaagggaa gatagaccga tgagcttcga
     1501 cgcttttccg ctgcgcggcg agagcgaccg ctcggcggtt ctacagctct ggcgagacaa
     1561 cttcggtgac cagcgcatca agaccaagat cgatccgcgc tggcactggc tgaccgaggc
     1621 gaacccctcg ggccccgcga agatttgcgt cgtaacgaag cgcgaaacgg gcgaggtcat
     1681 aggcagcgcc ggcgcaatgc cgcgcgtgct gcggatcggc gggagccccg tcttggcggc
     1741 tgttctctgc gatttcgtgg tcgacaagcg gcaccgcgtc gccggcgccg cgctcgcgct
     1801 tcagcgaaag ctcgccgacg cctgcttcgc cgacggcatc agcgtgctgt acggctttcc
     1861 gaacaagaac gcgtttccgg tgttcgcccg catcggttac aaaacggtcg gggcggccac
     1921 gatgatggcg cggccgctgc gcgccgggcc gcacctgcgc gatcacgtgc ccgcgctctt
     1981 ggccgagacg gccggcttct tcgtcgactg cgtattgcag gcctacgacc tgcagctcta
     2041 tctgcgccgc gcgcgcaacc tggacgatac tcagcggccg gccgcagacg actcgttcca
     2101 gactctctgg gacgaggtca aagtcgctcg ggcgatcgtc gccgagcgca cacccgcaca
     2161 cctgaactgg cgctacgcaa agcaccctgt cgacgactgc accttcttct gcctgaccga
     2221 gaagtcgacg ggccgcctgc gcggctacgt ggcctatacg gtcgcttccg ggaaggtcaa
     2281 cgtgctcgat gcgttctggg cctcgccgga ggtgcaaaag gccctgttcg tccgtttcgt
     2341 ttggcgcatg cgggcgctcg gccacgcctc catttcggtc tgttacgcgg gcgacgcgag
     2401 cgtcgcggcg acgctgcgag agctgggctt cattcagtcg aaggagagta ggaatttcgt
     2461 ttgcaaggtg gcgccccacg agttggatcg agtgggcccg gccgcctacg acttgtcgca
     2521 gtggtcgcta ttcgacggcg aactcgacat ttgagttcgg ctttgtgacc ggctgcggat
     2581 cgtggcaaga atccagatat gctagcccta ctgcggaagg tcgcgagtct cgcgggcgtc
     2641 ggcctgacgg cgctcggatg tgcctcgccg gttcccgaag agcagacgag tctggcccgc
     2701 tctcccggcg tggatccggg caagcctagc cgcccgattt cgatggtttg gcttcaccac
     2761 agcaccggcg ataggctgct gcgcggtggt ctgttcgatg ccctcaaggc cgacggtatc
     2821 caatttcacg acatcaatta caaagaggcg gtggtcgatg gcttcgtgat tggcgaccac
     2881 acggacgtgg acgactggcc caagactttc aacacgccga agtttttcga tacagtgaag
     2941 cgctgggagc tcggagccga caagcaggct cacgacatcg tgatgttcaa gagctgttac
     3001 ccgaattcgg atatcaagac cgacgcgcag ctcgaagact acaagcgcta cttcgactct
     3061 ctcgtgccca ctttccaagc caaccccgat attctgttca ttgcgatgtc cacgcccccc
     3121 ctggtcaagc gcaacaccag cgtggccgct gcgtcgcgtg cgcgcgagtg ggcgcgttgg
     3181 ctgacgtccg agtactcgac tcggctccga aacgtgaagg tgttcgactt gttcgatgct
     3241 ctcgcgatcg cggagggcaa ccccgatgcg aatacgttgg ttccacagtt cgcgagtgga
     3301 cgcggcgact cgcacccctc ccccgaaggc gcgcgcgccg tgacgcgtct gttcattccc
     3361 tggctgaacc gcacggctcg tgaagccggc ctgtccgact gatcataagg agcgtctcta
     3421 tccatgcccg cacccaacat cgaatcccgc atccgcgagt tcgtcgcctc caatttcgga
     3481 tttcgcggag ccagcctgaa catggatggg aacctagatc tgatccaaca ggggatattg
     3541 gattccacgg gcgtgctcga agtcgttgcg ttcctggaaa atgatctcgg tgtcaaagtc
     3601 gaagacgacg agatgatccc ggagaacctg gggacgatga gcaggatgat aagcttcgtg
     3661 tctcggaaga agggcagcag ctgaccttgg ctcagcgatg attcgcgaca gccgcagcga
     3721 cgaatttctg atcgacaacc ggctacgcgt gcacgccgcg cgcaagcccg accacgtcgc
     3781 tctccgcatc ggcgagcgct cgttcagcta cgcgcagctg aacgaggcgg ccgactcttg
     3841 ggcgaagctc ctgcgcgggt tgggcgttgc ccgcggcgat cgcgtcctca tcgcgctcga
     3901 caacgtctac gagaccgtcc tcggcttcta cggcgcgctg agggcggacg cggtgccgtc
     3961 gatcgtgggg gcgggccggc gcttcaagcg cctcgagcgc atcatcgcgc tcgcggagcc
     4021 gcgcgtgctc ctcaccaact cgaaagtggc gcaagggctc gggctcggcg tcactcggtc
     4081 gtgccgagtc gtgctcgtcg acgacgtcga gctcgaaggc gccatctcgc tcgccgcggc
     4141 gatgcgagag tgcggctccg aagcagcgcc gccagcgcgg caatccatcg agctcgatct
     4201 cgcgacgctg tgctggacca cgggctctac cggtgaaccc aaaggggtga tgctcaccta
     4261 ccagaacctg cggaacagcg cgtcggcgat cgcgagctat ctcgagcacg gcgacgacga
     4321 cgtcgtgatg tgcgtcttgc cgctgctgca cacttacggg ctgttccagc tgctcgtcac
     4381 gcatttcacg ggcggcacgc tggtgctcga ggcgggtttc tcgctcgcgg tgccgatttt
     4441 gaagcgcctg caagagatgc gggttacggg cttcgcgggt gtgccgacca tctacgcgtc
     4501 catcttggcc ctgaaggatt tggaccgctt cgacctgtcg aggctgcgtt atttgacgaa
     4561 cgccggttac gctttgccgg cggcgcacct ccggcgtttg caggagctct gtcccgagac
     4621 gcgcatctac gcgatgtacg gccagacaga gtgcacgcgc gtctgttact tacctcccga
     4681 gcaagcgctc gagcgaccgg cgtcggtcgg catctcgatg ccgaaccaag agctatggct
     4741 cgtccgtgaa gacgggaccc gggccgaaaa gggcgaaatc ggtgagctcg tggtgcgcgg
     4801 cccgaacgtc atgcgcggct actggcgcga tcccgagggg acggcgcgcg cgctgagacc
     4861 cggaaggttg cccggtgaag tggtgctgta cactggcgat ctgttccgca ccgacgagga
     4921 gggctacctc tatttcgtcg cgcgcgcgga cgacatcatc aagacgcgcg gggagaaggt
     4981 ggttccctgc gacgtcgagc aagcgataag ctctttgtcc gacgtggtgg aagtggccgt
     5041 gctcgggatc cccgatccga ttcatggagt cgccgtgaag gcggtcgtgg tcaagcgccc
     5101 gggctcgagc ctcaccaccg acgacgtcaa gcgcgtagta ttcacgtcga tcgacgaggt
     5161 cgcggtgccg cgggtggtcg agttcgtcga agcgttgccc cgatccgaag gtggaaaagt
     5221 catgaagagc agtcttctct gatgtgcggt gtcgcaggcc ttctgaacct cgggacgagc
     5281 cagccgccgc ccagccgcga gctcgctctg cgcatgtcgt ccatgctgca ccaccgcggt
     5341 cccgacggtt acggcatcta tcgtgaccgg cacgccgtgc tcgcgcaccg tcgcctctcg
     5401 atcatcgatc ttgagggcgg ctggcagccg atgaccgaca ccgacgagca ggtgtacatc
     5461 tgcgccaaca acgagatctt caactacatc gagctccgcc aagagctcga ggcaaaaggt
     5521 cggcgcttcc acagtcactg cgacaccgaa gtgatcgtgc aggcgtacct cgagtacggc
     5581 gcggaggtgg cgcagcacct caacgggcaa tgggccttcg cgttgtggga cacccgcacg
     5641 caggagctcg tgttgtcgcg cgatcgcgcc ggcatcctcc cgctcttttg gaccgtgcac
     5701 ggtaacgttc tgcgcttcgc gagcgaggtc aaagcgctgt tcgcggatcc cgaagtgccg
     5761 cgcgagtttt cgccggaggg cttggacgag gcgctcacgt tttgggccgc cgtcgcaccg
     5821 ttgacgccct atcgtggcgt gcagcagctg ccgccggggg tgaccgccat cttcaaagca
     5881 ggctcgaacg ttccgaagat cgtcgatcgc gtttggccca tgctttccgg tccggctcgt
     5941 cgcgcgagcg agaacgcgcc gcaggagcag cgggtcgaag cggtgcgcga gtcactcgag
     6001 caggcggcgc agcttcgcct gcgagcagac gtgccggtcg gcgcttatct ttcgggtggg
     6061 ctcgactcga cgatcctcac gtcactcatc gcggcgcacc gcgacgtgcc actccgaacc
     6121 ttctc
//
LOCUS       DASTKS010000081         4163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1351106, whole genome shotgun sequence.
ACCESSION   DASTKS010000081 DASTKS010000000
VERSION     DASTKS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351106"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFQ35_01745"
     CDS             <1..187
                     /locus_tag="VFQ35_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929373.1"
                     /translation="GAVAVSSLRSASALATGGVVLARPFQRGAHTRLERRFELLGSAR
                     APASSGTELAAGLSVMG"
     gene            complement(177..773)
                     /locus_tag="VFQ35_01750"
     CDS             complement(177..773)
                     /locus_tag="VFQ35_01750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9929374.1"
                     /translation="MNLALFDFDGTITTRGTYLDFIRFAVSRRRQVIGSAALSPCMAA
                     YRLGLVRERSLRPLVARVAFGGTPATTLRERGERFANIAIPPLVRDEAKRRIDWHKQQ
                     GDTIVVVSASLDVYLESWCRTHSLALICTQLEVRNGRATGRYVDGDCTGAEKKRRVLQ
                     RYEPERYDLVYAYGDTAEDDEMLSLAHRKFFRWQEVNP"
     gene            896..2200
                     /locus_tag="VFQ35_01755"
     CDS             896..2200
                     /locus_tag="VFQ35_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9929375.1"
                     /translation="MVDVLSDVERRELLAKPRPRELPIAPNAPKARRKLPLFERARDV
                     DREARPIYAVWEITLRCDLACRHCGSRAGHARSDELSTAECLNLVEQMAELGVLEVTL
                     IGGEAYLREDFPEIVRALVRHGMTVSMTSGGRGITPAMAALCKEAGMDSVSISLDGLR
                     ETHDRLRGVKGSFDSALEAMKNLRAAGLPVSCNSQINRLSLPDLPGVLELIAEHGGHG
                     WQLAMTVPMGRAVDEPEVLFQPYELLEVFPVLAELAERSKALGVRLWPGNNVGYFGPH
                     EGLLRKAYLRGHGTGCGAGRIVLGIEADGAIKGCPSLPTEAWTGGNIRDDSLRDIWER
                     SAPLRYTRDRTVDDLWGFCRTCYYADECRAGCTWTSFVFFGRAGNNPYCHHRALEMQR
                     AGKRERVVRRASAPGTPFDHGLFEIIEEDLSGAELAPMETSR"
     gene            2197..2877
                     /locus_tag="VFQ35_01760"
     CDS             2197..2877
                     /locus_tag="VFQ35_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929376.1"
                     /translation="MSHLVQCPGCQRHLRAEEKACPFCGAAVDFSDVAPPRLPAQRLS
                     RAAQIAFSATLAGSLAATACGAAEGGDGEQGGSGTLGGATGNAGHPNSGGQTPVPVYG
                     APIRGGTTGAGGAFPSGGTPNGGSPSGGAASGGRIGAGGRVGTGGDTAVPVYGAPIRG
                     GTTGAGGSGGASSGGRTGSGGATIMPVYGAPPRGGAPSTGGTSGKGGGSGNDAGGAGG
                     VSGAGDGA"
     gene            2928..3263
                     /locus_tag="VFQ35_01765"
     CDS             2928..3263
                     /locus_tag="VFQ35_01765"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HET9929377.1"
                     /translation="MNKRLRLRPEELKPLATQYGSCRASDKIVVDGEKVGFMFREEPD
                     EPHDSGWRFLSGTETEDYINQPGRLARYDVNFVANCDPEVVPHLGAAVGVAFERAGEA
                     FVETLAELP"
     gene            complement(3300..3740)
                     /locus_tag="VFQ35_01770"
     CDS             complement(3300..3740)
                     /locus_tag="VFQ35_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929378.1"
                     /translation="MAGGDDDGSGQVPVADVLAKRPGRARRVAAIVLAAVFFWSEGPK
                     LFGLAEAGWDVLWQQPKWKVHGEPPPPGDDGDARFMAKYGVPIESVGSCIVGRGTADY
                     AATYDAVIAVVKRIPHRYQDEYWFITVGAWSLGLLAVRLWKRAH"
     gene            3837..>4163
                     /locus_tag="VFQ35_01775"
     CDS             3837..>4163
                     /locus_tag="VFQ35_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929379.1"
                     /translation="MRAALLAGVAFPLSARAQSISIDEEHKKPREMPVAPPPSATVAK
                     APPAPVRPPSPLETPVEYPEGAQGEAEVVLILTVAVDGSVTNAEIASGAPPFAARAIE
                     SAKKWKF"
BASE COUNT          646 a         1279 c         1510 g          728 t
ORIGIN      
        1 gggcgcggtg gcggtgagtt cgttgcgctc ggcgtctgcg ctagcgacgg gcggcgtcgt
       61 gcttgcgcgc ccttttcagc gaggcgccca cactcgactc gaacgccgtt tcgagttgct
      121 cggcagcgct cgcgctccgg cgagctcagg tacggaactc gccgctggtc tatcggtcat
      181 gggttgactt cttgccagcg gaagaacttt cgatgagcaa gggagagcat ctcgtcgtct
      241 tcggcggtgt cgccgtatgc gtagacgagg tcgtagcgct cgggctcgta gcgttggagg
      301 acgcgtcgct tcttttcggc gccggtgcag tcgccgtcga cgtagcgacc ggtggctctt
      361 ccgtttctga cttcgagctg ggtgcaaatg agtgcgagtg agtgggttcg acaccagctc
      421 tccaagtaga cgtcgagcga agccgagacg acgacgatcg tgtcgccctg ttgcttgtgc
      481 caatcgatgc gacgtttggc ttcgtcgcgg acgaggggag ggatggcgat gttcgcgaag
      541 cgttcgccgc gttcgcgcaa cgtcgttgcg ggagtgccgc cgaacgcgac gcgcgcgacg
      601 agggggcgca agctgcgttc acggacgagg cccaaccgat acgcggccat gcaggggctc
      661 agggccgcgc ttccaatgac ttggcggcgg cgactcacgg cgaagcggat gaagtcgagg
      721 taggtgccgc gcgtcgtgat ggtgccgtcg aagtcgaaga gcgcgaggtt catgcgacgg
      781 cgatagattt agccggtcga gccgtgccga gggcgcattt ttccgctcgg gcgagttctt
      841 cgcgcgcgac gcgctggcga aggggaccgc ggtctgccag agtctgtgcg ggcgcatggt
      901 ggacgtgttg agcgacgtcg agcggagaga gcttttggcg aagccgcgtc cgcgcgagct
      961 gcccatcgcc ccgaatgcgc cgaaggcccg gcgcaagctg ccgctgttcg agcgcgcgcg
     1021 cgacgtcgat cgcgaggccc gaccgattta cgcggtgtgg gagatcaccc tgcgttgtga
     1081 tctggcttgc cggcactgcg ggtcgcgcgc ggggcacgcg cgcagtgacg agctcagcac
     1141 ggcggagtgc ctgaacttgg tcgagcagat ggccgagctc ggcgtgctcg aggtcacgct
     1201 gatcggcggc gaagcgtact tgcgcgaaga ctttccggag atcgtgcggg cgctcgttcg
     1261 tcacggcatg accgtgagta tgacgagcgg cggtcgcggc atcacgcctg ccatggccgc
     1321 gctctgcaaa gaagccggaa tggattcggt gagcatttcg ctcgatggtc tgcgcgagac
     1381 gcacgatcgt ttgcgcggcg tgaagggctc gttcgacagc gcgctcgagg cgatgaagaa
     1441 cctgcgtgcg gcgggcctgc cggtgtcgtg caactcgcag atcaatcggt tgagcctccc
     1501 cgacttgcca ggggtcttgg agctcattgc cgaacacggc ggacacggct ggcagctcgc
     1561 gatgacggtg ccgatggggc gcgccgtcga cgaacccgag gttctctttc aaccgtacga
     1621 gttgctcgag gtgtttccgg tcttggctga gctcgcggag cgctcgaaag cgctcggggt
     1681 gcgactctgg cctggcaaca acgtcggcta cttcggcccg cacgaagggc tcttgcgcaa
     1741 ggcgtacctg cgcgggcacg gcaccggctg tggcgcggga cggatcgtgc tcggcatcga
     1801 ggcggacggc gccatcaaag gttgcccgtc gctgccgacc gaggcgtgga ccggcggcaa
     1861 cattcgggac gatagccttc gcgacatttg ggagcgctcg gcgccgctgc gctacacgcg
     1921 cgatcgcacc gtcgacgacc tttggggatt ttgccggact tgctactacg cggacgaatg
     1981 ccgcgcggga tgcacctgga cgtcgttcgt gttcttcggt cgcgctggca ataaccctta
     2041 ttgtcatcat cgcgcgctcg agatgcagcg cgccggcaag cgcgagcgag tggtgcgtcg
     2101 ggcgagcgcc cctggcacgc cgttcgatca cggacttttc gagattatcg aagaagactt
     2161 gagtggcgca gagctcgcgc caatggagac tagccgatga gtcatctggt tcagtgcccg
     2221 ggttgccaac gtcacttgcg cgccgaagaa aaggcgtgcc cgttttgcgg cgcggccgtc
     2281 gacttcagcg acgtggcgcc gccgcgcttg ccggcgcaac gtctctcgcg cgcggcgcag
     2341 atcgcgttca gcgcgacgtt ggcgggttcg ctcgcggcga cggcctgtgg agctgccgag
     2401 ggtggcgacg gtgagcaggg cggtagcggg acactcggcg gtgctaccgg caacgccggg
     2461 catccgaata gtggcgggca gactccggtg ccggtctacg gcgctcccat ccgcggcggc
     2521 acgactggcg cgggcggcgc gttcccgagc ggcggcacgc cgaatggcgg cagcccgagc
     2581 ggcggtgcgg caagcggcgg ccgtatcgga gccggtggcc gcgtcggtac tggcggcgat
     2641 acggcggtgc cggtttacgg cgctcccatt cgcggcggca cgactggcgc gggtggttcg
     2701 ggtggcgcga gcagcggtgg tcgcaccggt tctggtggag ccacgatcat gcccgtttac
     2761 ggagctccgc cgcgcggcgg cgcgccaagc accggcggaa cttcgggcaa aggcggcggc
     2821 tcgggcaacg acgccggtgg tgccggtgga gtgagtggcg cgggcgacgg ggcctgagag
     2881 ccgagtcgtt ctggcctgcg cgccaatctg ctctatcgtt cgcgccgatg aacaagcgtc
     2941 tgcgcctgcg acccgaagag ctcaagcccc tggcgaccca gtacgggtcc tgtcgcgctt
     3001 cggacaagat cgtggtcgac ggcgaaaagg tcggcttcat gtttcgcgag gagccggacg
     3061 agccgcacga tagcggttgg cgcttcttgt cggggaccga gaccgaggac tacatcaacc
     3121 aaccgggtcg cctcgcgcgc tacgacgtga acttcgtcgc gaactgcgat cccgaagtgg
     3181 tgccgcacct gggcgcggcg gtgggcgtag cgttcgaacg cgctggcgag gccttcgtgg
     3241 agacgttggc ggagcttccc tgacgatagg cagtgcgagc ggcgcgacgc ggctcgcgct
     3301 cagtgagcgc gtttccagag tcgaaccgcg agcaatccta gcgaccaagc gccgacggtg
     3361 atgaaccaat attcgtcctg gtagcggtgt ggaatgcgtt tcactaccgc gatcaccgcg
     3421 tcgtacgtcg cggcgtagtc ggcggtgccg cgtccgacga tgcacgagcc gaccgattcg
     3481 atgggaacgc catacttcgc catgaatcgg gcgtcgccgt cgtccccggg agggggcggc
     3541 tcaccgtgca ccttccattt gggttgctgc cacaagacgt cccaacccgc ctccgccagg
     3601 ccgaagagct tcggcccttc tgaccaaaag aagaccgctg cgaggacgat ggcggcgacg
     3661 cgacgtgcgc ggcccggcct tttggcgagc acgtctgcca cgggcacttg cccgctgccg
     3721 tcgtcgtcgc cgcctgccac cgtgcggcca gtttggcagt ggttctgcga cctcgtaaag
     3781 cagaaagtcc tggtccatgg taaacggcag gttggatggt cgttggtcgg tgggcaatgc
     3841 gcgctgccct gcttgctgga gtggcgtttc ccctctccgc gcgagcccag tccatctcga
     3901 ttgacgagga gcacaagaag ccgcgagaga tgccggtcgc tccgccgcct tcggcgacgg
     3961 ttgctaaagc gccaccggct ccggttcgtc cgccgtctcc actcgaaacc cctgtcgagt
     4021 atccggaagg agcgcaaggc gaagccgagg tcgtgctcat cctcacggtt gccgtcgacg
     4081 gcagcgtgac caacgcagag atagcgagcg gcgcgccgcc cttcgccgcg cgcgccatcg
     4141 agtccgcgaa gaaatggaag ttt
//
LOCUS       DASTKS010000082         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1127948, whole genome shotgun sequence.
ACCESSION   DASTKS010000082 DASTKS010000000
VERSION     DASTKS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127948"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFQ35_01780"
     CDS             <1..884
                     /locus_tag="VFQ35_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET9929380.1"
                     /translation="AIESLLSATIADGMTGKKHDPDAELIGLGIGNIVVPFFGGIAAT
                     GALARTATNVRAGARSPIAAVVHSIVVLLAVLLFAPLVAYVPMASLAGLLLLVAWNMS
                     EVRHFANIVRIAPKSDVFILLQCYVLTVVFDMVIAVSVGVVMAALLFMRRTAELTHSR
                     ILEGKQSESEHRLAPGVALYEIAGPLFFGAAQNAMGAIGNIGADVRVIVLALGQVPSI
                     DATGFVALESSLNRLRVLGKQVILAGPLPQPHSVFDRANLPKHHNHVHFAASLDEALE
                     RARALAESNSPHQLQPA"
     gene            1465..3192
                     /locus_tag="VFQ35_01785"
     CDS             1465..3192
                     /locus_tag="VFQ35_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HET9929381.1"
                     /translation="MPYIDSATGEVVIRVVYGGVPGGGKSANLERLGEMVSAQRRGAI
                     VTGSADGAAEFDWLDFAGGYVDGRRVRCQLLALVGPTRIDERALLLKSADVFVFVVDS
                     RASETSRARILYSETVELLRTLGQAPELAVIVQANKQDEPGALSPQLVAAALGVRDAS
                     LVLPAVASTGSGVMETVILAARIATERVRALSIDALGELPSDESDPSRLQAKIEGMRE
                     AERKTKEEAERKVQEAKEEAERKAREAKEEAERKVKQEAERKARDEAERKARAEAERK
                     AQEAKEQGERKAQEAKEEAERKAKQEAERKARDEAERKARAEAERKAKQAQEETARRV
                     RAEAERQAKEAQEVAERKARAEAEHQAKEAQEAAERKARAEAERQAKEESQRPSKREE
                     PSARPLASSGPEPRTAIPAGRSSEPRLRPPMKPAQRSQPPAASPTAPDRSAPTGSSSQ
                     ARTNKDRAPKEREDDLHNEPTMPGWMPPAGALELQGGPAGSPAAQADDVGKSGAAEQT
                     AAASEQAAPAGEGEPLVAGGRAFEFTSDVAAETSLLAPAAGARAGFWSWLANFLAKLF
                     RAGSASRLV"
BASE COUNT          606 a         1010 c         1094 g          507 t
ORIGIN      
        1 gcgcgatcga gtcgctgctc tcggccacca tcgcggacgg gatgacggga aagaagcacg
       61 atccagacgc cgaattgatt ggtctcggta tcggcaacat cgtcgtcccg ttcttcggcg
      121 gaatcgccgc gaccggcgcg ctcgcgcgca ccgcgaccaa cgtgcgcgcg ggtgctcgct
      181 ctccgatcgc ggcggtcgta cactcgatcg tcgtcttgct cgcggtgctg ttgttcgcgc
      241 cgctcgtcgc ctacgtgccg atggcgtcgc tcgcggggct cctgttgctc gtcgcgtgga
      301 acatgtccga ggtgcgccac ttcgccaaca tcgtccgcat cgcaccgaag agcgacgtct
      361 tcatcttgct ccagtgctac gtgctcaccg tcgtcttcga catggtcata gccgtgtccg
      421 tcggcgtagt catggcggcg ctcctcttca tgcggcgcac cgccgagctc acccactccc
      481 gcatcctcga gggcaagcaa agcgagtccg agcatcggct cgcgccgggt gtcgcgctct
      541 acgagatcgc cggccctctc ttctttggcg cggcccagaa cgcgatgggc gccatcggca
      601 acatcggcgc ggacgtacgc gtgatcgtgc tcgcgctcgg gcaggttccc tccatcgacg
      661 cgaccggttt cgtcgccctc gagagctcac tcaatcgtct gcgcgtcctc ggcaagcagg
      721 tcatcctggc gggaccgctc ccgcagccgc actccgtgtt cgatcgcgcg aacttaccga
      781 agcaccacaa ccacgtgcac ttcgcggcga gcctggacga agccttggaa cgcgcccgcg
      841 ctttggccga gagcaactcc ccgcaccagc tccagcccgc gtgagcgagc ctcgtcgcgc
      901 tcgctgaacc tcccttggcc gaaaccgtcc gaccttcgtc cttttgccac gtccctcgcg
      961 cgctaccttc agcgcatgct ggagctccgt ccaacctgcg agcattgtaa taaaccgcta
     1021 cctccagagt cggccgaagc gcgtatctgc agcttcgagt gcaccttctg cgccgactgc
     1081 gtcgacaacg ttctcctcgg cgtttgtccc aactgcgctg gaggtttctg cgtacgcccg
     1141 actcgacccg ttcgcgagtg gcgccccggc gaatctctcg cgcagtttcc cgctagccag
     1201 aaggttcgac atcgccccgt cgatgtgcgc gcccacgcgg atttcgctga accaattcgc
     1261 accatttctc cgaccgcgcg ctaggtcgcg cgtcttttcg cggctcgagg ccatttatcg
     1321 ccacggccga ggaggtcgga ggacgtggcg gaaacattgt ttctcactct agcctacacc
     1381 ccgtaggtag gcttcgcccc aacctggcgg gatgttggat gacgcgtgac cgggatcgtg
     1441 agacagtcat cgctgggaga ggcgatgcct tacatcgatt cggcgactgg cgaggtggtc
     1501 atccgcgtcg tttatggcgg ggttcccggt ggcggcaaaa gcgccaacct cgagcgtttg
     1561 ggagagatgg tctcggcgca gcgacgcggc gcgatcgtga ccgggagcgc ggacggcgcg
     1621 gcggagttcg attggctcga cttcgccgga ggatacgtcg atgggaggcg tgtgcgctgc
     1681 cagctgctcg cactcgtggg cccaacgcgc atcgacgagc gagcgttgtt gctgaagtcc
     1741 gcggacgtct tcgtgttcgt ggtcgattca cgagcgagtg agacgagccg tgcacgaata
     1801 ctctactcgg agacggtcga gcttctcaga actctcggac aggcgccgga actcgccgtg
     1861 atcgtgcaag ccaataagca ggatgagccg ggcgcgctct ctccgcagct ggttgctgcc
     1921 gcgcttggag tccgtgacgc gagtttggtc ctgccagctg tagccagcac tggcagtggg
     1981 gttatggaga cggtgatttt agccgctcga atcgcgaccg agcgcgtgcg cgcgctgtcg
     2041 atcgacgcac ttggcgagct gccgagcgac gagtcggatc cgtccaggct gcaggcgaag
     2101 attgaaggaa tgagggaagc ggagcgaaag acgaaggaag aagccgagcg caaagttcaa
     2161 gaagcgaagg aagaagccga gcgcaaagcc cgagaagcga aggaagaagc cgagcgcaaa
     2221 gtgaagcaag aggctgagcg caaggcgaga gacgaagccg agcgcaaggc gcgagcggag
     2281 gccgagcgca aagctcaaga agcgaaggaa caaggggagc gcaaagccca agaagcgaag
     2341 gaagaagctg agcgcaaagc gaagcaagag gctgagcgca aggcgaggga cgaagccgag
     2401 cgcaaagcgc gagcggaggc tgagcgcaag gcaaagcaag cgcaggaaga gactgctcgc
     2461 agagtgcgcg cggaggccga gcgccaagcg aaggaggcgc aggaagtggc cgagcgcaaa
     2521 gcgcgcgcgg aggccgagca ccaagcgaag gaggcgcagg aagcggccga gcgcaaagcg
     2581 cgagcggagg ctgagcgcca agcgaaggag gagtcgcaac gcccgtcgaa gcgagaggag
     2641 ccgtccgctc gcccgctggc gtcgagtgga cccgagccgc ggacggccat tcccgcaggg
     2701 cgatcaagcg agcctcgatt gcggccgcca atgaagcctg cgcaacgcag ccagcctcca
     2761 gcggcgtctc ctactgcccc ggaccgctca gcgcccactg ggagcagctc gcaggcccgc
     2821 acgaacaagg accgagctcc gaaggaacgc gaagacgacc tacacaacga gccaacgatg
     2881 ccgggctgga tgccgccagc aggagcgttg gaactccaag gggggccagc gggtagtcca
     2941 gcggcgcagg cggatgacgt tgggaagtcc ggtgcagccg agcaaacggc ggccgcttcc
     3001 gagcaagcag caccggccgg agaaggggaa ccgctcgtgg ctggcggtcg ggcattcgag
     3061 ttcacgagcg acgttgctgc cgagacttca ctgctcgctc cggcggcggg ggcgcgcgcg
     3121 gggttttggt cgtggctggc gaactttctg gcgaagttgt ttcgcgccgg aagtgcgagt
     3181 cgactcgttt agcgggagtg ctctgcgcga cgtgtgt
//
LOCUS       DASTKS010000083         6232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1128070, whole genome shotgun sequence.
ACCESSION   DASTKS010000083 DASTKS010000000
VERSION     DASTKS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128070"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..776)
                     /locus_tag="VFQ35_01790"
     CDS             complement(144..776)
                     /locus_tag="VFQ35_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929382.1"
                     /translation="MKKKRTRIVPEIVLLSTVTSCVVPAVATGCGGDDDSGSQHGGAA
                     GASGAAGSSAGGQSNQGGQSNKGGVTGFGGFSVAAPFGGHTSNGGAPSGGSANGGATS
                     GGVSNGGTSNGGTSNGGTSSGGKQNQGGGAGGRTGGGFGGFTFSVAAPFGGRSESGGR
                     PGTGGGVAAGAFGNPGRGGTSGSGGTSGTSGNGGNAGKGGGTSGGGGTKP"
     gene            1033..2400
                     /locus_tag="VFQ35_01795"
     CDS             1033..2400
                     /locus_tag="VFQ35_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HET9929383.1"
                     /translation="MIGCLEATLDQSERDAVREELEGLLAFQRDLAVWRPLRPGSPVL
                     RGLTPIVSLYVHGELRGCQAHGGGAPEQRLARAFLAASADQRFRALTPDEREQIVAQV
                     SYARRLRQISVKHVKAEFVAGVHGLMLVAAEGPVLLVPDVARDLALDEEGFVGVLEQK
                     ANSPRDAWGKLELFAFETERVVARTDGYWARQPALPAVLNWLAQQVELDGRVRFGLDS
                     RSGESLHRGFLRHARTASVVQALSVDSTTAPTAARARTWLTNELERGHSGQTVDDWPD
                     DAAGIAATWALASLAGIRCEAVLGELARAPALHAEPWHAAQVVAALGQDAPTALWQVC
                     VDSLAREPFAPWTAIAAHRRSDEATFERAIRMLVERLPPREFGFVAEPALAGLTLEAL
                     ALSSNSEVGSRRAEALELLRQSQLWADSDPAPLASWVHGAFPLAANQSLLRCDASAHA
                     ALALR"
     gene            complement(2417..3703)
                     /locus_tag="VFQ35_01800"
     CDS             complement(2417..3703)
                     /locus_tag="VFQ35_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929384.1"
                     /translation="MIRAQRFGRGMVLAIGVGAVLTAASCAGLERNADDDGFGTGGVF
                     SPPAGGSLSVSGGATNSAEGGHAPSGSGGASGAASGGQSSAGAAGTGSGGAPTSSGGA
                     QSTGGATQGGSGGAASTCSDLPPNNGDTCAHAVEWGWCKESWLGNSCLRSCGKCNGSS
                     SGAGGSTTNGGASSSGGASNGSSGGSTGSVEQPPRIDGGSPGWASRYWDCCKPACGWK
                     GNVPGGSTPVSSCTAQGQSMGGNYDARNACESGGSAYMCQDFGPWAVSDTLAYGFAAV
                     SRSDYCGRCYQLQFTGKSHNATTDAGSASLSNKTMIVQAINNGGVGSDQFDLLIPGGG
                     VGDFDACSSSWGTSSLGERYGGFFLACQKQNGFNHDVSKACAKDKCQSVFANKPDLLR
                     GCLFFTDWFNAPDNPALVFKEVTCPAAITNKSGLKR"
     gene            complement(3895..5184)
                     /gene="hisD"
                     /locus_tag="VFQ35_01805"
     CDS             complement(3895..5184)
                     /gene="hisD"
                     /locus_tag="VFQ35_01805"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET9929385.1"
                     /translation="MNDVLFKSVESGSSEFDAALERLARRGESDLDRVEPVVREILET
                     VRREKDSALRRYVEKFERRTPGRLFVRDYGGREALAELDPVVRSALELGHARIKRYHE
                     VQREKQLAGFEYEEQGVTLGSRVSPLERVGVYAPGGKALYPSSVLMCAVPARVAGVTE
                     VYLACPQVNATVRAACYLAGVTGIVDAGGAQAIAAMAYGTESVPQVDKIVGPGNLYVT
                     AAKRLVFGEVDIDSLAGPSEILVVADHTADPRHVAADLLSQAEHDEAAYALLLTTSRE
                     LVERVRPELERQLASLPRRDIAEQSLRNNGLALVVSDRQALAAVANRIAGEHVAVQTE
                     SARELTSKIARAGALFIGPMTPEAAGDYVAGPSHVLPTGGAARFGAPLGVYDFVSRSS
                     VIEYTREALAAQTDAIAAFARAEGLEAHARAVEVRTR"
     gene            complement(5181..5789)
                     /locus_tag="VFQ35_01810"
     CDS             complement(5181..5789)
                     /locus_tag="VFQ35_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HET9929386.1"
                     /translation="MDSDLAESISALKNAFESRQVPVRFGKAEPAAVEALRSALRVPR
                     RYREFLLAVDPVDVETRTPVERVRLIPSSRLLEEQRGYALLETGEPRRGPAPNGWRPS
                     WVLIGHSTLLGDPYFLDVSSPDPEGDCPVYTAMSGTDQWKPKLCASSFAMFLRILAVS
                     MEVARGFAEEGLDMDDEQLFRESLGPKIRQYDPAAVKAGHWT"
     gene            complement(5955..>6232)
                     /locus_tag="VFQ35_01815"
     CDS             complement(5955..>6232)
                     /locus_tag="VFQ35_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929387.1"
                     /translation="PFGEHFGGYLEGSGGLARVSDDLLALYGYADATHLKPYFGGRLG
                     LEWFAPNPHLAVALSFGGRSYQQGLSRERSNEAAIALLGNIGIRYTF"
BASE COUNT          972 a         2110 c         2028 g         1122 t
ORIGIN      
        1 ggcgagcgcg agcgcctcgg gcgcgtactg ctctttggcg aactcttgga gcttgatgac
       61 gcgcgtacgg ccgcgcacgg ttgcaagctc gcggcgttgc tttcgcgcca agggaccttc
      121 cccgaaccga aacgcctggt ccgtcatggc ttcgtccctc caccgccgct cgtccctccg
      181 ccctttcccg cattaccgcc gttgcccgaa gttccgctag taccccccga gccgctagtc
      241 ccaccgcggc ccggattacc gaacgcgccc gccgcgacac ctccgccggt accgggccga
      301 ccaccgcttt cgcttcgtcc accgaagggc gcagcgacgc tgaaggtgaa tccaccgaat
      361 ccacctcctg tgcgcccgcc cgcgccgccg ccctggttct gctttcctcc gctagaggtg
      421 ccaccattcg acgtgccgcc gttcgacgtg ccgccattcg agacgcctcc gctcgtagca
      481 ccaccattcg cgctgccgcc gctcggagcg ccgccattcg acgtatgccc tccaaacgga
      541 gcggcgacac tgaatccgcc aaatccggtg acaccccctt tattcgattg tccgccttga
      601 ttcgattgtc cgcctgccga actccctgcc gcccccgaag ctccagcagc tccaccatgc
      661 tggcttccgg agtcgtcgtc gccgccgcag ccggtggcca ctgcgggtac gacgcagctc
      721 gtcaccgtgg aaagcaggac gatttcggga acgatgcgcg ttcgcttttt cttcatcggt
      781 tacctgtggc ccggagtgca aagcagggtt cgtgcccagc gtgatcgagc ggtattcagc
      841 ggaaatgagc cagtcccgtc ccgctgtgtg gcccagtctg tgccattggc gacgttccga
      901 cacgctgttg cccgggtgtc tggcgcagag tccacgctcg atgcacgtgg tcggcggtag
      961 aggtcccgaa aaggctttgc atccgagttc ccttttgcga atgcagttac tggtgcgagg
     1021 acttggggtc tgatgattgg ttgtctcgag gcgacgctag atcagagcga gcgagatgcg
     1081 gtccgcgagg agctggaagg cttgctcgca tttcagcgcg acctcgcggt ttggcgcccg
     1141 ctgcgccctg gctcgccagt gctgcgtggg ctcacgccga ttgtgtctct ttacgtgcac
     1201 ggtgagttgc gtggctgcca ggcccacggc ggtggggccc ccgagcagcg gttggcgcgt
     1261 gcgttcttgg cggcgagcgc ggaccaacga ttccgcgcgt tgactcccga tgagcgtgaa
     1321 caaatcgtag ctcaagtcag ctacgcgcga cggctgcgcc agataagcgt gaagcatgtc
     1381 aaagccgagt tcgttgcggg tgttcacggt ctcatgctcg tagctgcgga gggcccggtg
     1441 ctgctcgtcc ccgacgtggc acgcgacctt gcgctcgacg aggagggctt cgtgggcgtg
     1501 ctcgagcaaa aggcgaactc gccgagagac gcttggggaa agctcgagct cttcgccttc
     1561 gaaacggagc gcgtcgtcgc gcgcactgac ggttattggg cgcgacagcc cgcgctgccc
     1621 gcagtgctga attggctcgc acagcaggtc gagttggatg gaagggtgcg ctttgggctc
     1681 gactcgcgga gcggcgagag tctgcatcgt ggcttcttgc gtcacgcgcg tacagcaagt
     1741 gtcgtccagg cgttgtcagt cgactcgacc acggccccga ccgcggcgcg ggctcggacg
     1801 tggctgacga acgagctcga gcggggccat tcgggccaga ccgtggacga ctggcccgac
     1861 gacgccgcgg gcatcgctgc cacgtgggcg ctcgcgagtc tcgccggtat tcgctgtgag
     1921 gcggtattag gcgagctggc gcgcgcacct gcacttcacg ccgagccgtg gcacgcggcg
     1981 caagtcgttg ctgcgctggg gcaagacgcc ccgaccgcgc tttggcaagt gtgcgtggat
     2041 tcgctcgcgc gagagccgtt tgcaccctgg actgccatcg cggcccatcg gcgaagtgac
     2101 gaggcgacgt tcgaacgagc cattcggatg ttggtcgaac ggcttccgcc tcgcgaattc
     2161 ggcttcgtcg ccgaacccgc gctggctggg ctcacgctcg aagcgctcgc gctgtcttcg
     2221 aactccgaag ttggctcgcg gcgcgccgaa gcgctcgaac tcttacgcca gagtcagctt
     2281 tgggccgatt cggatccggc tccgctcgcg agctgggtgc atggagcctt cccgctcgcg
     2341 gcgaaccagt cactgttgag gtgtgacgcg tccgcgcacg ctgctctcgc cctgcgctga
     2401 aagcgtcggt tcacgctcag cgcttcaacc cggacttatt ggtgatcgcc gcggggcagg
     2461 tgacttcctt gaacacgagc gcggggttgt cgggcgcgtt gaaccagtcg gtgaagaaca
     2521 agcagccacg caagaggtcg ggcttgttgg cgaacacgct ctggcacttg tctttggcgc
     2581 aggccttcga gacgtcgtgg ttgaagccgt tttgcttttg gcacgcgagg aagaagccac
     2641 cgtagcgctc gccgaggctc gatgtgcccc acgagctcga gcacgcgtcg aagtcgccga
     2701 cgcctccgcc cggaatcaag agatcgaact gatccgagcc gaccccgccg ttgttgatgg
     2761 cttgcacgat catcgtcttg ttggagagcg aggcggagcc agcgtcggtc gtggcgttgt
     2821 gcgacttgcc ggtgaattgc agctgatagc agcgtccgca atagtcacta cggctcacgg
     2881 ccgcgaaccc gtaggcgagg gtgtcgctca ctgcccaagg cccgaagtcc tggcacatgt
     2941 acgcgctgcc gccgctctcg caggcgttcc gcgcgtcgta gttgccgccc atgctttgcc
     3001 cttgagcggt gcagctcgag accggtgtcg agccgccggg gacgttgccc ttccagccgc
     3061 acgcgggttt gcagcagtcc cagtaacgac ttgcccagcc cgggctgccg ccgtcgatgc
     3121 ggggcggctg ctccacgctg cccgtcgagc cgccgctcga gccgttgctc gcgccgccgc
     3181 tgctgctcgc gccgccgttc gtggtgctcc ctccggcgcc gctgctgcta ccgttgcatt
     3241 tgccgcacga gcgcaggcag gaattgccga gccagctctc tttgcaccaa ccccactcga
     3301 ccgcatgcgc gcaagtatcg ccgttgttgg gaggcaagtc gctgcacgtc gaggcggccc
     3361 cgccgctgcc gccttgggtc gcgccgcccg tgctctgcgc gccgccgctg ctcgtgggcg
     3421 cgcccccgga accggttcca gcggcgcccg cgctcgattg gccaccgctt gccgcgccgc
     3481 tcgcgccgcc gctgccggag ggtgcgtgtc cgccttccgc gctgttcgtg gcgcctccgc
     3541 tgacgctcag cgagccgcct gccggtggag aaaagacacc gccggtgccg aagccgtcat
     3601 catcggcgtt ccgctccagc cccgcgcacg aagccgcggt cagcacggcg ccgaccccga
     3661 tagccaacac cattccacgc ccgaaacgtt gcgctcgaat catcggtgct agccgaacta
     3721 acaccgccag cccacgtcgg cgagcctgtg ccgaaataac ttttcgtaat ctcgaccggt
     3781 taggaaacta tatttccggc cgcttctgtt gagttcgaaa aactacgagc tcggtgcgtg
     3841 tcgcttgctc ggcttgaacg cgattcgggt ctcgagcgac ctgcgtccga gcgctcagcg
     3901 ggtgcgcact tcgacggcgc gggcgtgcgc ctcgagccct tccgcgcgcg caaaagccgc
     3961 gatcgcatcg gtttgagccg cgagcgcctc gcgcgtgtac tcgatgaccg agcttcgcga
     4021 cacgaagtcg taaacgccga gcggtgctcc gaaccgcgct gcgccgccag tgggaaggac
     4081 gtgcgatggc ccagccacgt agtcgcccgc agcttcgggc gtcatcggtc cgatgaagag
     4141 cgcgccagca cgagcgatct tgctcgtgag ctcgcgagcg ctctcggttt gcacggcgac
     4201 gtgctcgccc gcgatgcgat tggcgacggc ggcgagcgcc tgacgatcgc tcacgacgag
     4261 cgcgagcccg ttgttgcgca acgactgctc ggcgatatcg cggcgaggaa gggaagcgag
     4321 ttgccgctcg agctcggggc gcacgcgttc gacgagttcg cggctggtcg tgaggagcag
     4381 cgcgtacgcg gcttcgtcgt gctccgcttg ggagagcaag tcggctgcga cgtgacgcgg
     4441 gtcggcggtg tgatccgcga ccaccaagat ttcgctcggg cctgccaggc tatcgatgtc
     4501 cacctcgccg aacacgaggc gcttggccgc ggtgacgtac aggttgccgg ggccgacgat
     4561 cttatcgact tgtggcacgc tctccgtccc gtacgccatc gccgcgatcg cttgggctcc
     4621 gcccgcgtcg acgatgccgg tgacgccggc gaggtaacaa gccgcccgca cggtcgcgtt
     4681 cacttgcgga cacgcgaggt acacttcggt gacgcccgcg acgcgagccg gcacggcgca
     4741 catcagcacg ctcgatgggt acagcgcctt gccgccggga gcgtagacgc ccacgcgctc
     4801 gagagggctc acgcggctgc ccagtgtcac gccctgctct tcgtattcga agccggcgag
     4861 ctgcttctcg cgttgcacct cgtggtagcg cttgatgcgc gcgtgcccga gctcgagcgc
     4921 cgaacgcact accgggtcga gctcggcgag ggcctcgcgt ccgccgtagt cgcgcacgaa
     4981 gagccgccct ggcgtgcgcc gctcgaactt ctcgacgtag cgacgcaggg cggagtcttt
     5041 ctctcgccgc accgtttcca agatctcgcg cacgaccggt tcgacgcgat cgagatcact
     5101 ttctccgcgc cgcgccaggc gttcgagcgc agcgtcgaac tcgctcgagc ccgactcgac
     5161 cgatttgaag agcacgtcgt tcatgtccag tggcccgctt tgacggcggc gggatcgtat
     5221 tggcgaatct tgggcccgag cgactcacgg aacagctgct cgtcgtccat gtcgaggccc
     5281 tcctccgcga agccgcgcgc gacctccatg ctgacggcca agatccgcaa aaacatcgcg
     5341 aagctcgagg cgcagagctt gggcttccac tggtcggtgc cactcatcgc cgtgtaaacc
     5401 ggacaatcgc cttccggatc ggggctgctc acgtccaaaa agtaaggatc cccgagcaag
     5461 gtgctgtggc cgatcaagac ccagctaggg cgccaaccgt tcggagcagg cccgcgtcgc
     5521 ggctcccccg tctcgagcag cgcgtaacca cgctgctctt cgagcaaacg cgacgaagga
     5581 atcagtcgca cgcgctcaac cggggtccgc gtctccacgt ccaccgggtc gaccgccaac
     5641 aggaactccc gataccttcg gggcactcgc agggcgctgc gcaacgcttc gacggcggcg
     5701 ggttctgctt tgccgaagcg gacggggacc tgtcgcgatt cgaaggcgtt ttttagagca
     5761 gatatcgatt ccgcaagatc gctgtccaag tcgcgaccct catttcattc cccgacgagc
     5821 gcagggacgg aaccggaccg gattgccggc cggaaagcca aaaacctaga gtggcgtccg
     5881 ttttggctcg ggcaaggggg cgacgcaagc caaagtttga cgctttttac gggttcgacg
     5941 tcgagtcgcc ctcttcaaaa ggtgtagcgg attccgatgt ttccgagcag tgcgatggct
     6001 gcttcgttgc tgcgctcacg gctgaggccc tgctggtagg agcggccccc gaacgagagc
     6061 gccacggcca aatgcggatt gggggcgaac cattcgaggc ccaaccgccc tccgaagtac
     6121 ggcttcaagt gggtcgcgtc ggcgtacccg tacagggcta gcaggtcatc gctcacgcgg
     6181 gcgagcccgc cgctcccctc gaggtaaccg ccaaagtgct ccccgaacgg ga
//
LOCUS       DASTKS010000084         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_904959, whole genome shotgun sequence.
ACCESSION   DASTKS010000084 DASTKS010000000
VERSION     DASTKS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904959"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFQ35_01820"
     CDS             <1..392
                     /locus_tag="VFQ35_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929388.1"
                     /translation="MLIDMAKTLGAAPTPEATVVASFAPPAGLIANTSITNALKFVAT
                     SERQAAVRTNQTLASIAAPELRALASSIEGMQTQHFVLLAALVTGVVAPGPTLDKTRA
                     SALFPSTLVLSHASHAGLDQAPPDYYR"
     gene            523..1788
                     /locus_tag="VFQ35_01825"
     CDS             523..1788
                     /locus_tag="VFQ35_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929389.1"
                     /translation="MAILALHALIAVAMFELLPRGFPISHPRFYANEAAPTLLLAISA
                     TIGAGLARGHPAAIALLAVFPGAWLGVAVAASVSFPITGGRVVVAAAVVAFILGALWL
                     NLARRVRRQWALTAIALSLGFGGGAALVRSQRAPAPSTRPHALTSPLPPELPSIDPKL
                     PAGVSVDAANGTIQLRSGAVELALLPLLEFESRSPDRFWTLFSPLLSERLAPPPSSER
                     RIRVESPDGSRLQVHASRALRGEVFSHLASFTRFSLRGHARLGISFSPCPAAVIDFVH
                     ADYPSGAPARFAYLDEASTFHVVEASSAEKGPFSPLATGPLRRGEPLSIRLLELSNAS
                     RRTLFELTFEDWSAELSTALSPTAGFGVAENAIEFGLASPAANSDAYVILNLAATSVG
                     RGWDSVGHAAGLYENRISVQSFETELHRE"
     gene            complement(1864..>2018)
                     /locus_tag="VFQ35_01830"
     CDS             complement(1864..>2018)
                     /locus_tag="VFQ35_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HET9929390.1"
                     /translation="GGVFEVLLDDALVFSRAERRRFPDPKELKQLVRDVVAPGRSLGH
                     SDAEVP"
BASE COUNT          294 a          715 c          649 g          360 t
ORIGIN      
        1 cgatgttgat cgacatggca aagacgctcg gcgccgctcc gacgcctgag gccaccgtcg
       61 tagctagctt cgcgccgccg gcaggtctca tcgcgaatac gagcatcacg aacgccctca
      121 aattcgtcgc gacgagcgag cgccaggccg cggttcgcac gaaccaaacc ctcgcctcga
      181 tcgcggcgcc cgagctccga gcactcgcga gctccatcga gggaatgcag actcagcatt
      241 tcgtcctact cgccgcgctc gtcaccggtg tcgtcgcacc tggcccgacc ctcgacaaga
      301 cccgcgcgag cgcgctgttt ccctcgactc tcgtgctctc gcacgcaagt cacgcggggc
      361 tcgatcaagc gccgcccgac tactaccgct agcgcgccac gcgagccgcc ttgatggcgc
      421 gcaagagcgc gtctaacctc gcgcggtgcg agcgatggcg tttagcgagg cggcatagtg
      481 cgagagccgg ccggagctcg acttctcgag gctcaaagcg cggtggcgat cctcgccctg
      541 cacgcgctca tcgccgttgc gatgttcgag ctattgccgc gcggctttcc catctcgcac
      601 ccgaggttct acgcgaacga agccgcgccc acactcttgc tcgccatttc ggctaccatc
      661 ggggccgggc tcgcgcgagg acatcccgcg gctatcgcgt tgctggccgt ctttcccgga
      721 gcgtggctcg gagtcgctgt ggcggcgagc gtgtcctttc cgatcacggg cgggcgggtc
      781 gtcgtcgctg ctgctgtggt tgcgttcatt ctcggcgcgc tctggctcaa cctcgcgcgc
      841 cgagttcgaa ggcagtgggc actcactgcg atagcgctca gcctcgggtt cggcggcggc
      901 gcggcactcg ttcgctcgca gcgagcgccc gctccgtcga cgcgaccgca cgcacttacg
      961 tcgccgctac cgccggagct cccgagcatc gaccccaagc tcccggcagg ggtttcggtg
     1021 gacgctgcga atggaaccat tcagctgcga agcggcgcgg tcgagctcgc gctgcttccg
     1081 ctgctcgagt tcgagagccg ctctccggat cgcttttgga cgctgttctc gccgctgttg
     1141 agcgagaggc tcgcgccacc tccgtcttct gagcggcgga ttcgcgtcga gtcgcccgac
     1201 ggctcgaggc tccaggttca cgcgagccgc gcgctgcgcg gcgaagtctt ttcgcacctc
     1261 gcctcgttca cgaggttctc gctgcgtggg cacgctcggc tgggcatctc cttctcgccc
     1321 tgccctgcgg ccgtgatcga tttcgtgcac gccgactacc cttcgggcgc acccgcgcgc
     1381 ttcgcgtacc tcgacgaggc gtccaccttt cacgtcgtcg aagccagctc agccgagaag
     1441 ggaccgttct cgccgctcgc gaccgggcca ctgcggcgcg gggagccgct ctcgattcga
     1501 ctgctcgagc tctcgaacgc ttcgaggcga acgctgttcg agctcacctt cgaggactgg
     1561 tcggccgagc tctcgaccgc gctctctccg accgcgggct tcggggtggc ggagaacgcg
     1621 atcgagttcg ggctcgcctc gcccgccgcg aactcggacg cgtacgtcat cttgaacttg
     1681 gccgcgacat cggtaggcag agggtgggac tcggttggtc acgcagccgg tctgtacgaa
     1741 aaccgaatca gcgtgcaaag cttcgagacc gaacttcacc gtgagtgagt cgtctcgttg
     1801 ctcgcccgtg caccggcgcg aagcgctcga tgggtactgc ttcgcggtga ggggctcgaa
     1861 ccctcagggc acttcggcgt cgctgtggcc caacgagcgg ccgggagcca cgacgtcgcg
     1921 aaccagctgc ttgagctcct tcggatccgg gaaacgccgg cgctcggcgc gcgagaagac
     1981 gagcgcgtcg tcgagcagca cttcgaagac accgccga
//
LOCUS       DASTKS010000085         7579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1128416, whole genome shotgun sequence.
ACCESSION   DASTKS010000085 DASTKS010000000
VERSION     DASTKS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128416"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFQ35_01835"
     CDS             <1..751
                     /locus_tag="VFQ35_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9929391.1"
                     /translation="HTVEVYRRTEASEHETQFLGFDFGGGQLLAPPPPPTRHLEIVGD
                     SITCGYGNEGADMNCHYSADTENHYLSYAAIAARNVNAELITVAWSGKGVVYNYGDDD
                     SSKVDPMPTYYERTLPERSNSQFDFSTWQADAVVVNLGTNDFSTDIDPSQSEFETAYK
                     TFVTRIRSKHPNAWILLTNGSMLSGTDLTTVRTYISNVVKAVNDPKVKSFEIQPQDGA
                     DGYGCDWHPSKKTHVKMADQLTSVLKSTLNW"
     gene            1005..2105
                     /locus_tag="VFQ35_01840"
     CDS             1005..2105
                     /locus_tag="VFQ35_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929392.1"
                     /translation="MQAFGTGALRGRAEPEDNREQAVWMPAFPLGSPGWRQSAEPLCV
                     QDRSGLGVAGELNSFGVWADARGVWTSASWGSVNNGGPTKVRFNDGHRWVTWLQDEVG
                     GRDTPIPATNQLSGFPNGPLMLLNYRSYDFSPSIAPADSVALLDSSSLTFEPPLGTSS
                     DSFAVAAFGIGATDGYALIQERVANPSSTVHRYRGEHTWSLVGPIYTSASAIWASDTQ
                     VLVATDRWLFRHAPSDSQFLPMPLAPVTEYHSVWAFSDDDIWAGTGDGQLAHFDGVGW
                     TLQSTGLNTPITRLWGSDGELFYISEAHFGRVRNGLPETLIADTAQVRFWDLWGRSSK
                     EVFITLTDPTLAKYRCGPKRILWFDGELFHTF"
     gene            complement(2122..3399)
                     /locus_tag="VFQ35_01845"
     CDS             complement(2122..3399)
                     /locus_tag="VFQ35_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929393.1"
                     /translation="MSVPDTHSAIRAVFAIESPKLIAKLARMLGDVGLAEELSQDALV
                     AALEQWPESGVPDNPGAWLMTVAKHHALNALSRRKRLERKHEELGYVVESEKRPGEVE
                     LVEALDDDIGDDLLRLVFTACHPVLPTEARVALTLRMLGGLTTEEIARAFLAPAPTIA
                     QRIVRAKRTLAEAQVPFEVPSGAELAPRLDSVLEVIYLIFNEGYAATAGDDWLRPELC
                     EDALRLGRTLARLAKTQPEVHGLVALMELTASRGAARVGPGGEPIRLLEQDRSRWDRL
                     YIQRGLDALALAQASLEGRPAGPYVVQAAIAACHARAVVAENTDWSRIAELYGELAAL
                     RPSPVVELNRGVAVAMASGPAAGLAIIDPLREEPSLANYHFLPSARGDFLEKLGRLPE
                     AREEFLRAASLTQNARQRTLLLERAERCAERLG"
     gene            complement(3581..3931)
                     /locus_tag="VFQ35_01850"
     CDS             complement(3581..3931)
                     /locus_tag="VFQ35_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9929394.1"
                     /translation="MRFMMTYTQDPNVVPTPEQMAEIGKFTEEMIKSGVVVMTGGLVR
                     PTKGTKVRYSGGKFSVTDGPFAETKELIDGFAVVEVASHEEAVKTAERFMRVAGDGTG
                     EILQMFVPGGPPPR"
     gene            complement(3958..4518)
                     /locus_tag="VFQ35_01855"
     CDS             complement(3958..4518)
                     /locus_tag="VFQ35_01855"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9929395.1"
                     /translation="MMRKVLIGSAALVAALGMAVVVQPATYHVERSLLVHAPAEAAHA
                     LVNDFHAWRQWSPFEDKDLAMRRTYGGAPAGVGATYAWASDGQVGEGKMTILQSSTRG
                     VNIELSFLKPMRATSTATFTFTKVGDDTKVTWAMDGENTLPSKVAHLFFNMDKALGAE
                     FERGLDKLKSAAERSVAANLETAKLK"
     gene            complement(4608..5675)
                     /locus_tag="VFQ35_01860"
     CDS             complement(4608..5675)
                     /locus_tag="VFQ35_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9929396.1"
                     /translation="MSIRWGIVGCGKVCEVKSGPAFQKAEGSELVAVMRRDVEQARDF
                     ARRHGVPTVHATAEALISDPNVDAVYIATPPGSHCELALQVAAAGKPAYVEKPMARNH
                     AECTRMVEAFARARQPLFVAYYRRALPRFLKVKEVLASGVLGTLRSVDVRYTSNGQSR
                     LDRTNLPWRVCAEQSGGGLFLDLASHTLDVLDFLLGPLESVRGDAWNVSRTSLVEDRV
                     SLEFVTPSGAKGAGLWDFTSSAYEDRIVFNGALGTLTLSTFGDGPIQLSARAGTETWS
                     LPNPPHIQQPLVQSIVDALVGRGACSSSGESAARTSLVMDRALAGYYGSREDEFWATP
                     SRWSERRGSGQVPDFKTDSSG"
     gene            complement(5678..6733)
                     /locus_tag="VFQ35_01865"
     CDS             complement(5678..6733)
                     /locus_tag="VFQ35_01865"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET9929397.1"
                     /translation="MTSRKLTRRRFGGLLATGGLLVAGSGCRKAAAAEVWVSAEDGGE
                     LVCLNAETGEVSARVHVGKRPRGIKVSRDHKSIFVALSGSPRGGPNVDESKLPPAERS
                     ADGVGVVDIAGKKLLRTHASGQDPESFDLSLDGKLLYISNEETAQLSVLDLAAGKVVQ
                     QVDVGEEPEGVTLRPDGKVVYVTCEGDKVVVAVDTKTLKEVARVPTGLRPRSITFDKV
                     GRTGFVCDELSAEVTVFSTDSHAVLSTIKIEEPNKMPRPMGGVLSPDGKWLYVSTGRG
                     GSVAVIDVAARKLDGIVSDVGARPWGIGVSPDGKRVFTANGPSNDVSIIDVASRRVEK
                     RVTVGGLPWGLVVTGGH"
     gene            complement(6785..7273)
                     /locus_tag="VFQ35_01870"
     CDS             complement(6785..7273)
                     /locus_tag="VFQ35_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9929398.1"
                     /translation="MSSSYKRRLLDTLVQKLTEERDRATSQANEAASGATHEENRAES
                     DKDMRSTEASYVARGQAERVAKLEQAITLLSRMELRDFSKGAGIEASALVTLSQAGKE
                     VTYFLVVAAGGERLTFEGKNLQTLATSSPLGQALLGLAPGDEAEVESPQGARYYSVTS
                     VT"
     gene            complement(7336..>7579)
                     /locus_tag="VFQ35_01875"
     CDS             complement(7336..>7579)
                     /locus_tag="VFQ35_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929399.1"
                     /translation="PLYNQGNFEACFHIYEGAAMDLERRLPKRCTGPRRALGEGRARA
                     AKLDDPSAQAWALRDTFDALFEVVARHDDAGERKSK"
BASE COUNT         1306 a         2444 c         2461 g         1368 t
ORIGIN      
        1 gcacacggtc gaggtttatc gtcggaccga ggcaagcgaa cacgagacgc agtttcttgg
       61 tttcgacttt ggaggaggtc agctgctcgc cccaccaccg ccgccgacac gccacctgga
      121 gatcgtcggg gattcgatca cctgtggata tgggaacgaa ggcgcggaca tgaactgcca
      181 ctacagcgcc gacacggaga atcattacct gagctacgcg gcgatcgccg cgcgcaacgt
      241 gaacgcggag ctcatcacgg ttgcgtggtc gggtaagggg gtcgtgtaca actatggaga
      301 cgacgactcc agcaaggtgg atccgatgcc gacatactac gagcgtacgc tgccggaacg
      361 ctccaatagc cagttcgact tctcgacttg gcaggcagac gcggtggtgg tgaacctcgg
      421 cacgaacgac ttctcgaccg acattgaccc gagccagagc gaattcgaga ccgcttacaa
      481 gacgttcgtc acgcgcattc gctcgaagca cccgaacgct tggattttgc tgactaacgg
      541 ctcgatgctg agcggaacgg acctgacgac ggtgcgcact tacatcagca acgtcgtgaa
      601 ggcggtgaac gatccgaagg tgaagtcgtt cgagatccag ccgcaagacg gggccgacgg
      661 ctacggttgt gattggcacc cgagcaagaa gacgcacgtg aagatggcgg atcagctgac
      721 gagcgtgctg aagtctacgc tgaactggtg acgccgtgag taactggggc aatcgggtag
      781 tcgcgcgtgg ttggccactg gtcacgttgt gggcgactgc gagcgcatgc ggtggtgagg
      841 ccagttcctc gaacaaaggc gccgggggtg cgagcgtagc gaccgcgggg gcgggggcga
      901 acgcttcgag ctgggatgcg acttctctcg gaggtgcgaa cgcttcgagt gggggtgcga
      961 cttctttcgg gggcgacgcg tcgcggggtc ggcgaacgac tgcggtgcaa gcgtttggga
     1021 cgggcgcttt gcgcggacga gccgagccgg aagacaatcg cgaacaagcg gtgtggatgc
     1081 cggcttttcc tctcgggagc cctggctggc gacagagcgc ggagcctctc tgcgttcaag
     1141 atcggagcgg gttgggggtt gccggcgagt tgaactcatt tggagtttgg gcggatgcgc
     1201 gcggagtatg gaccagcgcc tcgtggggct cggtgaacaa tggcggcccg accaaggtgc
     1261 gcttcaacga cgggcatcgc tgggttactt ggctccagga cgaagtgggc gggcgggaca
     1321 ctcctattcc tgcgacgaac cagttgagcg gatttccgaa tggaccgctc atgctgctca
     1381 attatcgctc ctatgacttc agcccgtcca tcgcaccagc ggattcagtt gcgctgctcg
     1441 actcctccag tctcactttc gagcctccct tgggcactag ctcggattcg ttcgccgtcg
     1501 cggccttcgg gatcggcgcg acggacggct acgccctgat tcaagagcgc gttgccaacc
     1561 caagcagcac cgtccaccgt taccgaggtg aacacacttg gagcctggtc ggcccgatct
     1621 acacgtccgc cagcgcaatc tgggcgagcg acacgcaggt tctggtcgcc acggatcgtt
     1681 ggctgtttcg gcacgcgccg tcggatagcc agtttttgcc catgccgctc gcgccggtaa
     1741 cggagtatca cagcgtttgg gcgttttcgg acgacgacat ttgggccggc accggcgatg
     1801 gccagctcgc gcacttcgac ggggtgggct ggactctgca aagcaccggc ttgaatacgc
     1861 ccatcacgcg cctgtggggc agcgatggtg agctcttcta catttccgaa gcgcacttcg
     1921 gccgcgtgcg gaatgggttg cccgaaacgc tcatcgccga cacggcgcag gttcgttttt
     1981 gggacttgtg gggtcgatca tccaaagagg tcttcatcac gctgaccgac cctacactcg
     2041 cgaagtaccg atgcgggccg aagcgcatcc tgtggttcga cggcgaactg ttccacacgt
     2101 tttgagctcg aattacgaac ttcagccgag gcgttccgcg cagcgctcgg cgcgctcgag
     2161 gagcagcgtg cgctggcgag cattttgggt cagcgacgcc gcgcgcagaa attcttcgcg
     2221 cgcttcgggg agccgcccga gcttctcgag gaagtcgccg cgcgcgctcg gcaggaagtg
     2281 gtaattcgcg agcgacggct cttcgcgcag cgggtcgatg atggcaagcc ccgcggcggg
     2341 cccgctggcc atggcgacgg cgacgccgcg gttgagctcg acgactggcg agggacgcaa
     2401 cgccgcgagc tcgccgtaga gctcggcgat gcgcgaccaa tcggtgttct cggcgaccac
     2461 cgcgcgcgcg tgacacgctg cgatcgcggc ttgcacgacg tacggccctg ccggtcgccc
     2521 ttctagcgat gcttgcgcga gagcgagcgc gtcgaggccg cgttggatgt agagccgatc
     2581 ccaacgggag cggtcttgtt ccaacaagcg aatcggctca ccgccgggac cgacccgcgc
     2641 ggctccgcgc gaagcggtaa gctccatcag cgcgaccaag ccgtgaacct ccggctgagt
     2701 tttcgccagg cgcgcgagcg tgcgcccgag gcggagcgcg tcttcgcaaa gctcgggacg
     2761 aagccagtcg tcgccggcgg tcgcggcgta gccttcgttg aagatgaggt aaatgacttc
     2821 gagcaccgaa tccaggcgcg gcgcgagctc ggcgccgctc ggcacttcga agggaacctg
     2881 cgcctctgcc agcgtgcgct tggcacgcac gatgcgctgc gcgatggtcg gcgctggcgc
     2941 caagaaggcg cgcgcgattt cctcggtggt gaggccgccc agcatacgca gcgtgagcgc
     3001 gacgcgggcc tcggtgggca gaaccgggtg acaggcggtg aagacgagcc gcaagaggtc
     3061 gtcgccaatg tcgtcgtcga gcgcctcgac gagctcgacc tcgcctggac gcttttctga
     3121 ctcgacgacg taaccgagct cttcgtgctt tcgctcgagc cgcttgcggc gtgacagcgc
     3181 gttcagcgcg tggtgcttcg cgacggtcat gagccacgcc ccagggttgt cgggcacgcc
     3241 agactcgggc cattgctcga gcgcggcgac gagcgcatct tgggagagct cttcggcgag
     3301 gcctacgtca ccgagcatac gggcgagctt ggcgatgagc ttgggcgact cgatcgcgaa
     3361 gacggcacga atggcgctgt gagtatccgg gaccgacacg agacccggat gacacagcac
     3421 gttcgcgcgc cggcgcaagg cctctcgacg agcgacgcga atttagctcc gctcgggacc
     3481 ggtcgagctc ggatcgctcg cgtccgagct cacaccgctt gcgtcggagc cgggcgccct
     3541 cgccgatcgc accgcctcga atttctccga ggagcaacgt tcagcgcgga ggcgggccac
     3601 ctggcacgaa catttgcaag atctcgccgg tgccgtcgcc ggcgacgcgc atgaagcgct
     3661 cggcggtctt cacggcttct tcgtgtgagg cgacctcgac gaccgcgaag ccgtcgatga
     3721 gctctttggt ctccgcgaac ggtccgtccg tgacgctgaa cttgccgccc gaatagcgca
     3781 ccttggtgcc cttcgtgggc cgcaccaagc cccccgtcat caccacgacg cctgacttga
     3841 tcatctcttc ggtgaacttg ccgatttctg ccatctgctc gggggtgggc accacgttcg
     3901 gatcttgggt gtaggtcatc atgaatcgca tggctgttcc tttcaggctt tcacggctca
     3961 cttcaatttc gcggtttcta ggttcgccgc gacggagcgc tccgccgcgc tcttcagctt
     4021 gtcgagccca cgctcgaact cggcgcccaa cgctttatcc atgttgaaaa agaggtgggc
     4081 gaccttgctg ggcagcgtgt tctcgccatc catggcccac gtcaccttgg tgtcgtcgcc
     4141 gaccttcgtg aacgtgaagg tcgcggtgct agtggcacgc atcggcttca agaacgaaag
     4201 ctcgatgttc acgccacgcg tgctgctctg caatatggtc atcttgccct cgccgacttg
     4261 gccatcgctc gcccaagcgt aggtagcgcc gactcccgcc ggtgctccgc cgtacgttcg
     4321 ccgcatcgcg agatccttgt cctcgaacgg cgaccactgc ctccaagcgt ggaagtcgtt
     4381 caccaaagcg tgggcagcct cggcgggagc gtgcacgagc agcgagcgct cgacgtggta
     4441 cgtagccggc tgaactacca ccgccatacc gagcgcagcc accagagccg ccgaaccaat
     4501 tagaactttg cgcatcatgt cgtcacctgc ttcgcgagcc cgacttgggc ttccgagagg
     4561 acgtcaactg acgcaggctt ggatcgacac gaggccgacg atttccttca tccggaagaa
     4621 tctgtcttaa aatcgggcac ttggcctgag ccgcgccgct ccgaccaacg agacggtgtc
     4681 gcccagaact cgtcctcgcg ggaaccgtaa tagccggcca gcgctcggtc catgacgagc
     4741 gacgtccggg ccgcgctctc gccgctgctg ctgcatgctc cgcgcccgac gagagcgtcg
     4801 acgatgctct gaaccaacgg ctgttggatg tgtggggggt tcggcaatga ccaagtctcg
     4861 gtcccggctc gagccgagag ctggatgggc ccatcaccaa acgtcgacaa cgtcagcgta
     4921 ccgagcgctc cgttgaatac gatgcggtct tcgtacgcgc tgctcgtaaa gtcccagagc
     4981 cccgcgccct ttgctccgga gggcgtcacg aactcgaggc tcacccgatc ttcgacgagg
     5041 gaggtgcgcg atacgttcca ggcgtctcct cgcaccgact cgagcgggcc caagagaaag
     5101 tccagcacgt cgagcgtgtg gctcgccaga tcgaggaaga gcccaccgcc ggattgctct
     5161 gcgcaaacgc gccaaggcag gttcgttcgg tcgaggcggc tctggccgtt gctcgtgtag
     5221 cgcacgtcga cgctgcggag cgtcccgagc acaccgctcg ccagtacttc tttgaccttc
     5281 agaaaacgcg gcagcgcgcg tcgataatag gcgacgaaca agggctgacg cgcccgcgcg
     5341 aacgcttcga ccatgcgcgt acactccgcg tggttgcgcg ccatcggctt ctcgacgtac
     5401 gcgggcttac cagcggctgc cacttgcaag gcgagctcgc aatggctgcc cgggggcgtc
     5461 gcgatgtaga cggcatcgac gttcggatcc gaaatcagtg cttcggcggt ggcgtgaacg
     5521 gtcggcacgc cgtgccgtcg agcaaaatcg cgcgcctgct ccacatcgcg ccgcatcacc
     5581 gcgacgagct cactgccctc ggccttttga aacgcgggcc cgctcttcac ttcgcagacc
     5641 ttgccgcacc cgacgatgcc ccaacgaatc gacatggcta gtggccgccg gtcacgacca
     5701 aaccccaggg cagcccaccg acggtcacgc gcttttcgac gcgccggctc gcgacgtcga
     5761 tgatggacac gtcgttcgac ggaccgttcg cggtgaacac tcgcttgcca tccgggctga
     5821 cgccgatgcc ccaaggacga gctccgacgt cggagacgat gccatcgagc ttgcgggccg
     5881 cgacgtcgat gacggcaacg gagccgcctc gaccagtgct cacgtagagc cacttcccgt
     5941 ctggagagag cacgccgccc atcggtcgcg gcatcttgtt cggctcttcg attttgatgg
     6001 tggagagcac ggcgtgagag tcggtggaga acacggtgac ctccgcgctg agctcatcgc
     6061 aaacgaagcc agtacggccc accttgtcga aggtgatcga gcgcggacga agaccggtgg
     6121 gaactcgagc gacctctttc agggtcttcg tgtccaccgc gacgacgacc ttgtcgcctt
     6181 cgcacgtgac gtacacgact ttgccgtcgg gcctcaaagt gacgccctcg ggctcttcgc
     6241 ccacatccac ttgctgaacc actttgcccg cagcgaggtc gagcacgctg agttgcgccg
     6301 tttcttcgtt ggagatgtaa aggagcttgc cgtcgagcga gaggtcgaac gattccggat
     6361 cttgcccgct cgcgtgggtc cgcaaaagct ttttcccagc gatgtcgacg actcccacgc
     6421 cgtccgcgga gcgttcggct ggcggcagct tggactcgtc cacgttcggg ccaccgcgcg
     6481 gcgagccgct caacgcgacg aagatcgact tgtggtcgcg cgagaccttg atgccgcgcg
     6541 ggcgctttcc gacgtgaacg cgcgcgctca cctcgcccgt ttcggcattg aggcacacga
     6601 gttcgccgcc gtcttcggcg ctgacccaga cttctgccgc ggccgctttg cggcaaccgc
     6661 tgcctgccac caagagaccg cccgtcgcga gcagccctcc gaatcgtcga cgtgtcagct
     6721 tgcgagatgt catgggtttc ggcgccgcgt ccgacggagt tatcagggcg atgcgcggcg
     6781 cgcgtcaagt caccgaagtt acgctgtagt agcgggcacc ttgaggcgac tccacctcgg
     6841 cttcgtcgcc gggggccaag ccgagcagcg cctggccgag cgggctcgag gtcgccagcg
     6901 tttgtaggtt cttcccctcg aacgtcaagc gctcaccccc cgccgccacc accaagaaat
     6961 aggttacttc tttgcctgcc tgcgaaagcg tcacgagcgc ggacgcttcg attccggcgc
     7021 ctttcgagaa atcgcgcagc tccattcggg ataaaagggt tatcgcctgc tcgagcttcg
     7081 cgacgcgctc ggcctgccca cgcgcaacgt acgacgcctc ggtcgagcgc atgtccttgt
     7141 cgctctcggc gcggttctcc tcgtgagtgg cgccgctcgc ggcttcgttg gcttgcgacg
     7201 tcgcgcggtc ccgctcctcc gtgagcttct ggacgagcgt gtcgagcagc ctccgtttgt
     7261 aagacgaact catgctggcc gagagcaaag cagagtcgtg agggccgctc aaggccccga
     7321 gggcgacggc gagacctact tgctcttgcg ctcgccggcg tcgtcgtggc gcgcgacgac
     7381 ctcgaacagc gcgtcgaacg tatcgcgtag cgcccacgcc tgagcgctcg ggtcgtcgag
     7441 cttggccgcg cgcgcccgac cttcgccgag ggctcgcctc gggcccgtgc aacgctttgg
     7501 caggcgccgc tcgaggtcca tcgccgcgcc ctcgtagatg tgaaagcacg cctcgaagtt
     7561 gccctggttg tagagcggc
//
LOCUS       DASTKS010000086         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1574811, whole genome shotgun sequence.
ACCESSION   DASTKS010000086 DASTKS010000000
VERSION     DASTKS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574811"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFQ35_01880"
     CDS             complement(<1..297)
                     /locus_tag="VFQ35_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929400.1"
                     /translation="MHSQQSLADAARPAPEPETSGVEGLSNRCLGVLDLPRFHLASSE
                     WRGGEPLPTRSTADGDGSPPPLIWDRVPGDPRSFVLICEDPDAPRPTPFVHWLVY"
     gene            531..1964
                     /locus_tag="VFQ35_01885"
     CDS             531..1964
                     /locus_tag="VFQ35_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9929401.1"
                     /translation="MDQDLNVDVAIIGGGITGVTAAWLLANAGKSVALLEGRTIAGGV
                     TERSTAHATEAIDTRYASLERRGGDVARLVRESSRAAIERIFELARLCGAEASVRRVP
                     GYLFSEDPSESDALKAEAEAARRAGARVEVCAVPLPLAKAAGVRFDDQAELDPFAYTT
                     ALAARIPSSTAQIYEESMVVNVKPGDTCRLELEHGPSVLARDVIVATHYPATKTTFQT
                     KLAQYRSYVVAAATEKPLDALFWDTADPYHYVRSARVGETSYLIVGGEDHKTGQDARG
                     GPDGAFERLETYARRFDVNPSVRWSAQVVESVDGLPYIGQPTRGERIYVATGYGGNGT
                     TFGTLGAMLLTDALLGRDNPYAELYRATRFKPLTSLPPLVEENVDFPLHLLRDRLHAS
                     PKGSPATLSNGQAAVFHVDGERVAVYRDDDGRLHAVSAICTHFGCQVNFNSSEKTWDC
                     PCHGSRFDVDGAVLDGPATKRLARRLL"
     gene            1977..>2591
                     /locus_tag="VFQ35_01890"
     CDS             1977..>2591
                     /locus_tag="VFQ35_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET9929402.1"
                     /translation="MATRSKFPGAEEFLPKRRTLNTLREASKACRGCDLYIPATQTVF
                     GEGPSSARVVFVGEQPGNSEDLAGRPFVGPAGALFDRALAAAKIDREQVYVTNAVKHF
                     SFEPRGKARLHKRPRPGEVRACAPWLRAELSLIEPEVLVLLGSTAAQSLFGPSFRITQ
                     ERGKKLESELAPFVLATIHPSAVLRAPDEAAREQAFASLVDDLKV"
BASE COUNT          444 a          876 c          859 g          412 t
ORIGIN      
        1 gtaaacgagc caatgcacga aaggcgtcgg acgcggagca tccgggtcct cgcaaatcag
       61 gacgaatgag cgcggatcgc cgggcacgcg atcccaaatg agcggcggcg gggacccatc
      121 gccgtccgcg gtcgagcgag tgggaagcgg ctcccctccg cgccactcgg agcttgccag
      181 gtgaaagcga ggcaaatcga gcacgccgag gcaccgattc gagagacctt cgactcccga
      241 cgtctcgggc tcgggggcgg gacgagcggc gtcggccaga gattgctgag agtgcatggt
      301 gcctggctca gcaacgatcg tgccccggcg cgagagtcgc cgtgcacacc ggcgagctcg
      361 gtttgagaca gacaacactg ccccagtgcg gcggcgcgtc gtccggatta ctgcgagtct
      421 ccgcgcgtca acgcgactcg gttgcggtcc gcgcgttgca gggcgctcag gcgatgcgag
      481 caaccgatcc ggtgtggcgc aatgcgacgc gcgcgcctcg ttacccccgc gtggaccaag
      541 acctgaacgt cgatgtcgcc atcatcggcg gagggatcac cggcgtgacc gccgcttggt
      601 tgctggccaa cgcgggcaag agcgtcgcgt tgctcgaagg gcgaaccatc gccggaggcg
      661 tcacggagcg cagcaccgca cacgctacgg aggcgatcga cacgcgttac gcgagcctag
      721 agcgtcgcgg cggcgacgtg gcgcgcctcg tgcgagagtc gagccgcgcc gccatcgagc
      781 gcattttcga gctggcgagg ctgtgtggtg ccgaagcgag cgtccggcgc gtacccggct
      841 acctgttcag cgaggatccg agtgagagcg acgccttgaa ggcggaggcc gaagccgcgc
      901 gtcgcgccgg cgctcgcgtc gaggtctgcg cggttcctct accgctcgcc aaagcggccg
      961 gcgtgcgctt cgacgatcag gcggagctcg acccattcgc ttacacgacc gcgctcgcgg
     1021 cgcgcatccc gagctcgacc gcgcagatct acgaagaatc gatggtggtg aacgtcaagc
     1081 ccggagacac ttgtcggctc gagctcgagc acggtccgag cgtgctcgcc cgagacgtca
     1141 tcgtggcgac tcactacccc gcgaccaaga cgacctttca aacgaaactc gcgcagtatc
     1201 gttcgtacgt ggtcgcagcc gccaccgaaa agccgctcga cgcccttttc tgggataccg
     1261 ccgaccctta tcactacgtg cgctcggccc gcgtcggcga gacttcttac ttgatcgtag
     1321 gtggagagga tcacaagacg ggacaggacg cccgcggagg tccggacggc gccttcgagc
     1381 gcctcgagac gtacgcgcgc cgcttcgacg tgaacccgag cgtgcgctgg tcggctcagg
     1441 tcgtcgagtc cgtcgacggc cttccctaca tcggacaacc aacgcgcggc gagcgcatct
     1501 acgtcgcgac cggctacggc ggtaacggca cgaccttcgg cacgctcggc gcaatgctct
     1561 tgaccgacgc gctgctcggc cgagataacc cttacgccga gctctatcgc gcaacccgct
     1621 tcaagccact cacctcgttg ccgccgctcg tcgaagagaa cgtcgacttc ccgctgcacc
     1681 tactgcgcga ccgtttgcat gcttcgccca agggctcgcc cgcaacgctg tcgaacgggc
     1741 aggccgcagt ctttcacgtg gatggggagc gggtggccgt ctaccgcgac gacgacgggc
     1801 ggcttcatgc cgtttcggcc atctgcactc acttcggctg ccaagtgaac ttcaactcga
     1861 gcgaaaagac gtgggactgt ccgtgccacg gctcgcgctt cgacgtcgac ggtgcggtgc
     1921 tcgacggacc ggcaaccaag cgcctcgcgc gacgcttact ctgaattggt ggcgccttgg
     1981 cgacgcgcag caagttcccg ggcgccgagg aattcctccc caagcgccgc acactcaaca
     2041 ccttacgcga ggcatcgaaa gcttgtcgcg gttgcgactt gtacattccc gcgacgcaga
     2101 ccgtcttcgg cgaagggccg agctccgcgc gcgtcgtgtt cgtcggtgag caaccgggca
     2161 actcggaaga cctcgcgggc cgtccgttcg tcggccccgc cggcgcgctt ttcgaccgcg
     2221 cgctcgcggc cgcgaagatc gatcgtgagc aggtgtacgt cacgaacgcg gtgaagcact
     2281 ttagcttcga gccgcgcggc aaggcgcgct tgcacaagcg cccgcgcccg ggtgaagttc
     2341 gagcctgcgc gccgtggctg cgggcagagc tttcgctcat cgagccagag gtgctcgtcc
     2401 tcttgggcag caccgcggcg cagtcgttgt tcgggccgag ctttcgcatc actcaggagc
     2461 gcggaaagaa gctcgagagc gaactcgcgc ccttcgtgct cgcgacgatt catccgtctg
     2521 ccgtactccg cgcacccgac gaggccgctc gtgagcaggc gttcgcgtcg ctcgtggacg
     2581 atctgaaggt c
//
LOCUS       DASTKS010000087         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_14062,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000087 DASTKS010000000
VERSION     DASTKS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14062"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1673)
                     /gene="hutH"
                     /locus_tag="VFQ35_01895"
     CDS             complement(156..1673)
                     /gene="hutH"
                     /locus_tag="VFQ35_01895"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET9929403.1"
                     /translation="MSSDLILTGGPLQLADVERVARGRAAVRLSPTAIEKLQRSREAL
                     EQSIRAGGAIYGVNTGFGSLARQRLEGAQLQEVQRNLILSHAAGVGEFLPEDVVRAML
                     LLLAASLARGLSGVRPIVAERVVALLNGGVTPLVPAVGSVGASGDLAPLAHAALVLLG
                     EGEATLAGQRLSGAEAISELGLLPLVLEAKEGLALINGTHLMAAQGALFFADVERLQR
                     AALAASAMSIDACRGTDAFLDARVHAARGQVGQRDVAERVARLIEGSQIVPSHLHDDP
                     RVQDPYSLRCVPQVVGAAFDVIAFARSLVERELGAVSDNPLVFSEEGARAVVSAGNFH
                     GMPLAIALDTAVLGLCHIAGIAERRVYYILAASDAQNPLNPHLSPIPGLHSGLMIAQY
                     VAAACCNEIIGLTLPSSASNLPTSAGMEDYNSFGATSAAKARRALERATDVIAIELLC
                     AAEALEYQRPLKSGPGVERAHQTVRSVVSRRTADRPPSPDIAKIRALIQEGAFDG"
     gene            complement(1681..>2266)
                     /locus_tag="VFQ35_01900"
     CDS             complement(1681..>2266)
                     /locus_tag="VFQ35_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929404.1"
                     /translation="GSTGSLRAVEVRLPVPQVASPSVPEKPELRAVPRRGKLVSALLG
                     LGLLGGAVYVQVSHARQPALPMRAAVAPPPVIARSVELSPAAVASPAPTPSDVVAPEA
                     TPVVHDERALGFAMRWGTKQAELCHQRGGSAGRTVRVDLTFEPNGKVSRAALNGEEAP
                     NSAEDRCILGQFRGVMIPRFAGEAVAITREIKLR"
BASE COUNT          392 a          799 c          745 g          330 t
ORIGIN      
        1 ccgcgcatcg atcgacgcgc cgacctgttc aagctcgttt cgctgttgcc cgccgaccag
       61 gcgtcgagcg gccccggcga cggaccgctc ggtcctcagc cgggaaacag ctgaaatcgc
      121 cacccgtacc agcgtcgatg cgttgcgacg gcgcgtcagc catcgaacgc gccttcctga
      181 atcaacgcgc gaatcttggc gatgtcaggc gacggcggac gatccgcagt gcgccgcgaa
      241 acgacgctcc gcaccgtctg atgcgcgcgc tcgacgcctg gccccgactt cagcggtcgc
      301 tggtactcga gcgcctccgc cgcgcacaag agctcgatcg cgatgacgtc ggtcgctcgc
      361 tcgagcgcgc gccgggcctt cgccgccgag gtcgccccga acgagttgta gtcctccatc
      421 cccgcgctgg tcggtaagtt ggatgcgctc gagggaagcg tcagcccgat gatttcgttg
      481 cagcacgccg cggcgacgta ctgcgcgatc atcagcccgg agtgcagccc tggaatcggc
      541 gacaaatgcg ggttgagcgg gttctgcgcg tcggaggcgg cgaggatgta atagacgcgc
      601 cgctcggcga tccccgcgat gtggcacagt ccgagaacgg ccgtatcgag cgcgatggcg
      661 agcggcattc cgtggaaatt gcctgccgag accacggcgc gcgcgccttc ttccgaaaag
      721 acgagtgggt tgtcgctgac ggcgccgagc tcgcgctcga ccagtgaacg cgcgaacgcg
      781 atcacgtcga acgccgcgcc cacgacttgg ggcacgcagc gcagcgagta aggatcctgc
      841 acgcgtgggt catcgtgcaa atgactcggt acgatctgac tgccctcgat caagcgcgcg
      901 acccgctctg cgacgtctcg ttgtcccacc tgtccccgcg cggcgtgcac gcgagcatcg
      961 agaaatgcgt cggtgccgcg gcaggcatcg atcgacatcg ccgaggcggc cagcgccgcg
     1021 cgctgcaagc gctcgacgtc ggcaaagaac agcgctccct gcgcagccat caggtgcgtt
     1081 ccgttgatga gcgcgagtcc ctccttggcc tcgaggacga gcgggagaag gccgagctcg
     1141 gagatcgcct ccgcgccgct caaacgctgc ccggccagag ttgcctcgcc ctcgcccaac
     1201 agcacgagcg cggcgtgcgc gagcggcgcg aggtcaccgg acgctccaac cgagccgacc
     1261 gcaggcacga gcggggtgac gcctccgttc agcaacgcga cgacgcgctc cgcgacgatc
     1321 ggacgcaccc cggaaagccc gcgcgcgagc gaggccgcga ggagcaacaa catcgcgcgc
     1381 accacgtcct ccggcaggaa ctcaccgacg ccggccgcgt gcgacaagat gaggttccgc
     1441 tgcacctctt ggagttgcgc cccctcgagc cgctgccgag cgagtgagcc aaagccggtg
     1501 ttcactccgt agatcgcgcc gccggcacga atcgattgct cgagcgcctc acgcgagcgc
     1561 tgaagcttct cgatggcagt cggggaaagg cggaccgccg ctctcccacg cgcgacgcgt
     1621 tcgacgtcgg cgagctgcag agggcctccc gtgaggatca aatccgagct catgctcgac
     1681 ttatcgtagc ttgatttcgc gggtaatagc gaccgcttcg ccggcgaagc gcgggatcat
     1741 gacgccgcga aactgcccca ggatgcagcg atcttcggcg ctattcgggg cttcctcacc
     1801 gttcagtgcg gcgcggctca ctttgccgtt cggctcgaac gtaaggtcta cgcgaacggt
     1861 ccgacccgcg ctgccgccgc gctgatggca aagctccgcc tgcttcgtac cccaacgcat
     1921 cgcaaacccg agcgcccgct cgtcgtgaac aacgggagtt gcttcgggcg ctacgacgtc
     1981 gctcggcgtc ggggcgggac tcgccaccgc ggccggtgaa agctcgaccg accgagcgat
     2041 cacgggcggc ggagcgacgg ccgcacgcat cggtaacgct ggttgccgcg cgtgcgacac
     2101 ctgcacgtag accgctccac caagcaaccc gagaccgagc agcgcgctca cgagcttgcc
     2161 ccttctcggt acggcacgga gctcgggctt ctctggaacc gaaggcgacg cgacttgcgg
     2221 caccgggagt cgcacctcga cggcacggag cgaaccggtc gaaccc
//
LOCUS       DASTKS010000088         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_235771, whole genome shotgun sequence.
ACCESSION   DASTKS010000088 DASTKS010000000
VERSION     DASTKS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235771"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFQ35_01905"
     CDS             complement(<1..1002)
                     /locus_tag="VFQ35_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929405.1"
                     /translation="MPASAQTETIRIDYKAVPGCPSAEDFNAEVMKRTQSARLSESDA
                     RVRTFIVEIEQRRGGLIGSLRVREGRTETVAREVQGDRCADVASTLALATALAIDPHA
                     STSPAASASNGSAANPPTTGGAGATGDADAGPQAAGASRDKDGKAGEKSETRSPPRAP
                     EDSFDFDPSVMRSNGEHHVAFELGPSLLVGVTPGVAFGGSISAQMSNSRSGALLSAYG
                     ADLTLLRAPTSETAGASATFQLLYARPIACGIQLGHYDGISLSPCLGADVGVISGWGS
                     DLPHEESHTKLWAALELFARLRIGGKNDWSLEAQAGPTVPLTRYDFVFQEPRTQVHTV
                     "
     gene            complement(1313..1834)
                     /locus_tag="VFQ35_01910"
     CDS             complement(1313..1834)
                     /locus_tag="VFQ35_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9929406.1"
                     /translation="MSREFPSFTHTSAPEAARPALSSSLRSFGAIPAPLARYASSPLM
                     LNTALANLRAFEESSLSELEREILAVTMGRANGCQFCVSMHLNQLGRLRADPELVRAL
                     RAGDALPSPRLEALRQFVLNALEKRGDVDESAFASFREHGFDHRQALDTLLGIAAYTL
                     TTFANRLTETSEV"
     gene            complement(1921..2373)
                     /locus_tag="VFQ35_01915"
     CDS             complement(1921..2373)
                     /locus_tag="VFQ35_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9929407.1"
                     /translation="MKDQFQGDRIVLENALPFVIHGLYQQIRSVTYREFNEQGLELTP
                     EQWIVLVRLWERDRQSPSALSESTLRDRPTMTRILDTMEGNGLIVREVDEKDARARIV
                     CLTREAKALQKRLVPIAKRLVDRLEEGVPERDLEITRRTLRRMFENLR"
     gene            complement(2520..>4420)
                     /locus_tag="VFQ35_01920"
     CDS             complement(2520..>4420)
                     /locus_tag="VFQ35_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929408.1"
                     /translation="VALVGSDPYVHEGSDVTLIFRVRNKAAFETGLALSLAKRAERRG
                     ELEQSSLVHEGAKIEVTRSRDGRIRRHRASLNDLELVSNSPNAIKRVLSTIAGHHASL
                     ANEPDFRYLTARDAEVRTDAFAYVSDEFVAAVTGPAQKIGEARRQIQLAELSAPGYAA
                     LVAGWLDGRAPSSTKALQTAKWLSGSELRHADGAPIHFEPGAEASSSHGTPARLEPLL
                     DSPAVTRVTAEERRAYESFARGYTNLWTDRVDPIALSVDLEDGASGRTLTADLRILPL
                     LRREYREWLDVVGNARVSVPNLASGARIVAAIGQDARVRALLQGFGEDFLEDHKLRFD
                     WLGDYVVIGASNRNELANPLLSQVQDEIERPRRAAERETRDFEPAKLPLYAIVSLKSS
                     LAAGLALTALRQKASEAAPGAISWGRAAAYRGHEVVLVAAHERDEKINLYYALLPSAF
                     VIALNEASLHEAMDQVLDHPPVAEGTPKGSVSRAGQVVVELASEPSSALHRVAAAVGT
                     AALVHGRRSGCALAEALLRGAPETQNSPERLRALSRAYFGVVPLTPDGREYEYAPEGA
                     RDPVRGTDFAPTYPDVPVPGSPLQRVLAALRFARSDQSFDPEPGHTQEVPLQSLHLRA
                     TVTTARAK"
BASE COUNT          729 a         1398 c         1486 g          807 t
ORIGIN      
        1 taccgtgtgg acctgggtgc gcggttcttg aaacacgaag tcgtagcgag tgagaggcac
       61 cgtcgggccc gcttgcgctt ccagcgacca gtcgttcttc ccgccgatgc gcaggcgagc
      121 gaagagctcg agcgcagccc aaagcttcgt gtggctctct tcgtggggta gatccgagcc
      181 ccaccccgag atcacgccga cgtctgcgcc gaggcacggg ctcagcgaaa tcccgtcgta
      241 gtgcccgagc tggatcccgc aagcgatggg gcgcgcatac aggagctgga aagtggcgga
      301 ggcgcccgcg gtctcgctgg taggtgcgcg cagcagcgtg aggtcagcgc cgtacgcgct
      361 cagtaaggcg ccgcttcgcg agttactcat ctgcgccgaa atggaaccgc cgaatgcgac
      421 ccccggtgtc acgccgacga gcagggacgg tccgagttcg aaggcgacat ggtgttcgcc
      481 gttactgcgc atcacgctcg gatcgaagtc gaatgaatct tcgggcgcgc gcggcggcga
      541 gcgcgtctcc gatttttctc ccgctttgcc gtccttgtcg cgcgacgcgc cagcggcttg
      601 cggcccagcg tctgcgtctc ccgtcgctcc ggctccgccc gtggtaggcg ggttggcggc
      661 actgccattg ctcgcggacg cagctggcga agtactcgcg tggggatcga tggcgagggc
      721 agtggcgagc gccagcgtag aagcgacgtc ggcgcagcga tcaccctgga cttcgcgcgc
      781 gaccgtctcc gttcgccctt cgcgcactcg cagactgccg atcaagcccc cgcggcgttg
      841 ctcgatttcg acgatgaaag tcctcacccg agcgtcgctc tccgagaggc gcgcgctctg
      901 ggtccgcttc atgacctcgg cgttgaagtc ctctgcgctg ggacagcctg gaaccgcctt
      961 gtagtcgatg cgtatcgtct cggtttgagc ggacgcgggc attgcccaca agccacagta
     1021 caaaagtccg gcctggacga cccgtcgacc acgcgtgcgt cgcggcttgc gctcactgct
     1081 ctcaggtact ccgacgacgg tgcttgccct ttccagatga ggtttttatc cccgaggacc
     1141 gagcgcgaca accccgagct cgaccgcggc gaggcgtccc gccgtccgga gagcgtcggg
     1201 gcgcctcgcc tggcgcgtgt cagcagactt ttgtggcgta ccaattctcg cgcggggcga
     1261 gaaaccgaga gaaatttcct cgatttgcga cactgcgggc gcccgccaca cctcacactt
     1321 cggaggtttc ggtcaaccgg ttagcgaagg tcgtgagcgt atatgcggcg atcccgagca
     1381 gagtgtcgag cgcctgacgg tgatcgaagc cgtgttcgcg aaagctcgcg aacgcactct
     1441 cgtcgacgtc gccgcgcttt tcgagcgcgt tcaacacgaa ctgccgcagc gcctccaatc
     1501 tcgggctcgg caaagcgtcg ccggcccgca acgcgcggac gagctctgga tcggcgcgga
     1561 gtcgaccgag ctggttcaag tgcatcgata cacagaactg gcaaccgttc gctcgtccca
     1621 tggtgacagc cagaatttct cgctcgagtt cggatagcga gctctcttcg aatgctcgca
     1681 agttggcgag cgcggtgttg agcatcaagg gagagctcgc atagcgcgcg agcggtgcgg
     1741 ggattgcccc gaacgaacga agtgaactcg atagggcggg ccgagcggct tctggcgcgc
     1801 tcgtatgcgt gaaggagggg aactctctgg acatactgct ttcttccttt ctcgttgtga
     1861 ttgtcattgc aactattccg gtgaagaaga tcggcgctcc cggaaggggc ctcgccgcgc
     1921 tcagcgtaag ttctcgaaca tgcgccgaag cgtgcgccgc gtaatctcga gatcgcgttc
     1981 gggcacgcct tcttcgagac gatccaccaa gcgtttcgcg atcgggacga gccgcttctg
     2041 tagagctttc gcttcgcggg tgagacagac gatgcgcgct cgcgcgtctt tttcgtcgac
     2101 ctcccgaaca atcaggccat tgccctccat cgtatccaag atccgcgtca tcgtgggccg
     2161 atcgcgcagg gtactctcac tcaaggcgga gggactctgc cgatcacgtt cccataaacg
     2221 tacgaggacg atccattgtt cgggcgtaag ttcgagcccc tgctcgttga actcccggta
     2281 cgtcaccgag cgaatctgct ggtacagccc gtggatcacg aacggcagcg cattttctag
     2341 gacgatgcgg tcgccttgga actgatcttt catgagctcg tgacggaact ataagccgga
     2401 cggaacagtt gtcaatgcaa tcaaaacttc ggcgtcgcgg tcgagcgggc ggcacccgag
     2461 ccgtcggtgg cggcggactc gcgagcggct cggaggttgc gcgggggcgc gctcggctct
     2521 cacttcgcgc gggcggtcgt gaccgtggct cgcagatgca ggctttgcag cgggacctct
     2581 tgcgtgtgac caggctctgg gtcgaacgat tggtcgctgc gggcaaagcg tagggccgcg
     2641 aggacgcgct ggagaggaga gcccggaacg ggaacgtcgg gataggtcgg cgcaaagtcg
     2701 gtgccgcgaa cggggtcgcg agcgccctct ggcgcgtact cgtactcccg cccgtcgggg
     2761 gtcagtggga ccactccgaa gtaggctcgt gagagcgcgc gcaggcgctc gggcgagttc
     2821 tgagtttccg gagcgccgcg cagcagtgcc tccgccaagg cgcaacccga acgacgaccg
     2881 tgaacgagag cggcggtgcc cacggctgcg gcgacgcggt gcagggcgct tgaaggctcg
     2941 ctcgcgagtt cgaccacgac ttgcccagcg cgtgagacac tgcccttggg tgtgccctcg
     3001 gcaaccggcg ggtggtcgag cacctgatcc atggcttcgt ggagtgacgc ctcgttcaag
     3061 gcgatcacga aggcagacgg tagcagggcg tagtagaggt tgatcttctc gtcgcgctcg
     3121 tgagcggcga ccagcacgac ttcgtggcct cggtaggccg cggcccgtcc ccagctgatt
     3181 gctcctggcg cggcttcgga cgccttttga cggagcgcag tcagggcgag ccccgccgcg
     3241 agcgagctct tcagcgagac gatcgcgtag agcgggagct ttgcgggctc gaaatctctc
     3301 gtctcccgct cggcggcacg tcgtgggcgc tcgatttcgt cctggacttg cgacaacagc
     3361 gggttcgcga gctcgttgcg attcgacgcg ccaatcacca cgtagtcgcc gagccaatcg
     3421 aagcgtagct tgtgatcttc gaggaagtcc tccccgaacc cttggagcaa cgctcgcacc
     3481 cgcgcatcct gaccaatggc cgcgacgatc cgcgcgccgc tcgccaggtt cggtacggag
     3541 acgcgcgcgt tccccacgac gtcgagccac tctcgatact cgcgacgaag cagcggcaat
     3601 atgcgcaagt cggcggtcaa cgtgcgcccg ctcgcgccgt cttccagatc taccgagagc
     3661 gcgatcgggt cgacgcggtc cgtccacaga ttcgtgtacc cgcgcgcgaa cgattcgtag
     3721 gcgcgtcgtt cctccgcggt gacgcgcgtt acggcgggag agtcgagcag gggctccaag
     3781 cgagccggag tcccgtgcga cgagctcgct tcggcaccgg gctcgaagtg aatcggcgcg
     3841 ccatccgcgt gacggagctc gctcccagac agccacttgg ccgtttggag agctttggtg
     3901 gacgagggcg ctctgccgtc caaccagccc gcgacgagcg cggcgtagcc aggcgcggag
     3961 agctcggcga gctggatttg tcggcgggct tctccaatct tttgggcggg gccggtgacg
     4021 gcggcgacga actcgtccga tacataggcg aacgcgtcgg ttcgcacttc ggcgtcgcgc
     4081 gcggtgagat agcggaagtc cggttcgttc gcgaggctcg cgtgatgtcc ggcgatcgtg
     4141 gacaggactc gcttgatcgc gttcgggctg ttcgaaacga gctcgaggtc gttcaggctc
     4201 gcgcggtgac gacggatgcg gccgtcgcgg ctccgcgtga cttcgatttt cgcgccttcg
     4261 tggacgaggc tcgactgttc gagttcgccg cgtcgctccg cgcgcttggc caacgaaagc
     4321 gcaagccccg tttcgaacgc ggctttgttc ctcactcgga agatcagcgt gacgtcgctg
     4381 ccctcgtgca cgtaagggtc gctgccgacg agcgcgacgt
//
LOCUS       DASTKS010000089         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1128973, whole genome shotgun sequence.
ACCESSION   DASTKS010000089 DASTKS010000000
VERSION     DASTKS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128973"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..506
                     /locus_tag="VFQ35_01925"
     CDS             147..506
                     /locus_tag="VFQ35_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929409.1"
                     /translation="MKEATRTAWSARVAEWKQSGKTADEFAADKPYKGSTLVWRAWQL
                     RPGKGDGVRKERVRARPMPNRTAPEIKLAEVVRRAPKKTTTSGVTVEVVGARISVDRG
                     FDPTLLREVVRALRGEG"
     gene            503..865
                     /gene="tnpB"
                     /locus_tag="VFQ35_01930"
     CDS             503..865
                     /gene="tnpB"
                     /locus_tag="VFQ35_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9929410.1"
                     /translation="MIPSGVQVFVALEPVDMRWSFDKLSGAALERIGYDARSGALFIY
                     FGKRKTSVKVLFFDGSGMCCLYKRLDRGVFRLPEACAPDARYVELEEGLLDALLDGVD
                     LGEPPKPKRRRKPRVPVH"
     gene            926..>2136
                     /locus_tag="VFQ35_01935"
     CDS             926..>2136
                     /locus_tag="VFQ35_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9929411.1"
                     /translation="MSGELDPTSVRVEELQALLQRMELQLATTNAKLASVTTERDNLR
                     RAYRQLMEQFELLRRRIFVAKAERVDATQLELEFEATKQKLAALAEKLGADAPSSDAT
                     DTDADSDTDAETSEQSGSNGVGSRPAGKPPGTGSSQPKGRRNLANLKHLKERRVDIRD
                     AELEESGAEFIGWELSYKLGYQRPEPVRVVIARAKYKVPAAVPEAKPLPDDATVVEQD
                     GKRFTIITAPLPKLLIRRGLLAPSMLARIIVHKFRFGMPYYRQEEQFAADRIELDRGT
                     MARSVEEVGACLGAIVLACADDARKHAFCLNTDATGVAIRPEPLPDGKRQACAKGHFF
                     VVLADKKHVFFEYQPKHTSAAVCEMFRGFSGYIQADAHAIYDALFRGEAVDDESVPPT
                     EVACWSHARRR"
BASE COUNT          416 a          656 c          696 g          368 t
ORIGIN      
        1 tggctcgcga cttgggtgag gtgggcgcgc acgtgggacg tgtccgacat cgcccagctg
       61 ctagcggagt tcgatgagcg cattccgtat ccggtgcacc gaccccgtcg ttgaagagag
      121 cggcttagaa atgcgactcc gagcgcatga aggaagcgac gaggacggcg tggtcggcgc
      181 gggttgcgga gtggaagcaa agcggaaaga cggcggacga gttcgcggca gacaagccgt
      241 acaagggttc gacgttggtt tggcgcgcgt ggcagctgcg accgggcaaa ggtgacggcg
      301 tgcggaagga gcgtgtgcga gctcgaccga tgccgaatcg taccgcgccc gagatcaaac
      361 tggccgaggt ggtccgacgc gcaccgaaga aaactacgac gtcgggcgta accgtcgagg
      421 tggtcggggc gcgcatctct gtagaccgcg gattcgatcc gacgctgttg cgagaagttg
      481 ttcgagcgct tcgaggtgag gggtgattcc gagcggcgtg caggtgttcg tggcgctcga
      541 gccggtagac atgcgttggt cgttcgacaa gctttccggc gcggctctcg aacgcatcgg
      601 ttacgatgcg cgctcgggcg cgttgttcat ttacttcggc aagcgcaaga cttccgtgaa
      661 ggtgctgttc ttcgacggct ccggcatgtg ttgcctctat aaaaggctcg atcgcggcgt
      721 atttcggctg cccgaagcct gcgctccaga cgcacgctac gttgagctcg aggaaggact
      781 gctcgacgcg ctgctcgatg gagtggatct cggtgagcca ccgaagccga agcgacgccg
      841 caaaccgcgc gtgcccgtgc attgatgcat ggcgcgctgt tcaggctcga caagtcgatc
      901 tcagttcggt acagccaggc tcgtcgtgag cggcgagttg gacccaacct cagtgcgcgt
      961 agaagagctc caagctctgt tgcagcgcat ggagctccaa cttgcaacaa cgaatgcgaa
     1021 gctcgcgagc gtaaccaccg agcgcgacaa tttgcggcgc gcttatcgac agctgatgga
     1081 gcagttcgag ctcctgcggc ggcgcatctt cgtcgccaag gccgagcgcg tcgacgcgac
     1141 gcaactggag ctcgagttcg aagcgaccaa acagaagctc gccgcgctcg cggaaaagct
     1201 cggagccgat gcgccctcga gcgacgctac cgacaccgac gccgactccg acaccgacgc
     1261 tgaaacgtcg gaacaatccg gctcgaatgg cgtgggatcg cgccctgccg gtaagccccc
     1321 agggacaggg tcgtcccagc cgaaaggccg tcgaaacctc gccaacctga agcacctgaa
     1381 ggaacggcga gtcgacattc gcgatgccga gctcgaggag agcggcgcag aattcatcgg
     1441 ttgggagctc agttacaagc tcggctacca acggcctgag cccgtccggg tcgtcatcgc
     1501 acgcgccaaa tacaaggtgc ctgcggcggt gcccgaggcg aagccattgc cggacgacgc
     1561 caccgtcgtc gagcaagatg gtaagcgctt caccatcatc actgcgccgc tacccaaact
     1621 gctcattcgt cgcggcttgc tcgcgccgag catgctcgct cgcatcatcg tgcacaagtt
     1681 tcgcttcggc atgccgtact atcggcaaga ggagcagttc gcggcggacc gcatcgagct
     1741 cgatcgcggc accatggctc gctcggtcga agaggtgggc gcatgcctcg gcgccatcgt
     1801 gctcgcttgt gccgacgacg cgcgcaaaca cgccttctgc ctcaacaccg acgccaccgg
     1861 agtcgcgatc cgaccggagc cacttccgga tggtaagcgg caagcgtgcg ccaagggcca
     1921 cttcttcgtc gtccttgccg acaagaagca cgtcttcttc gagtaccagc ccaagcacac
     1981 gagtgctgct gtctgcgaga tgtttcgcgg cttctccggt tacattcaag ccgacgcaca
     2041 cgctatctac gacgcgctct tccgcggcga agccgtcgac gacgaaagcg tcccgcctac
     2101 cgaagtcgcc tgttggagtc atgcgaggcg acggtt
//
LOCUS       DASTKS010000090         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1352705, whole genome shotgun sequence.
ACCESSION   DASTKS010000090 DASTKS010000000
VERSION     DASTKS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352705"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFQ35_01940"
     CDS             <1..716
                     /locus_tag="VFQ35_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HET9929412.1"
                     /translation="AGVPGARTNRPDAEDGKAATATPAAGGGTMRRSHTRNWEVERVT
                     QKTLTPPGELHRLSVAVLLNGRWEQRGPKSVFVPRSPEEVKVLEEVVKRAVGFNAERG
                     DNVLVQAAQFSRVEEPTVAAEPRWVVATKTYWPVAAGVAALLGIATVVLVWRRSSKQA
                     AKALELKAASSTAALNGVAGAPELGAGVTPAELPAAPGMSFEDIRAQALELAAKDPAT
                     AAVVLRQWLGSAPAAAAAP"
     gene            778..1773
                     /locus_tag="VFQ35_01945"
     CDS             778..1773
                     /locus_tag="VFQ35_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET9929413.1"
                     /translation="MEVVTTMDLSGPQKAVLMLLSMDEAAATPILAELDAEEVKRLRE
                     AAAGLRSVPTNALEGVYTEFIQRSQEAVAVPGGGVPYLRRLAGRALGEQVAQEIFLEG
                     VEGPMERLANADPSALASVLEHEHPQLIAAVVSQLDNERAARVLEQLPVAMRPAVLER
                     LGNMREIPGALLEEVARAISAELPNSNPEAVRSVDGVARSAALVRRMDRQVGEALLGT
                     LEEGNSELAVEIRRAMYTFEDLGALDARALRSLLEAVPVEKLTVALKTASDSLKSHVF
                     TSMSKRAAERIREDMESMGGVRLAEVEAAQREIVEVALRLDAEGTISLENDNAMV"
     gene            1760..2512
                     /locus_tag="VFQ35_01950"
     CDS             1760..2512
                     /locus_tag="VFQ35_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HET9929414.1"
                     /translation="MRWFDPSYPHGAQAARFADISAGKRKIPSWVPSRPEGPRRNESS
                     LRPPKLPSEFVDAVRQELDQEYAPRMRASMVPDAIQARLSERPAAAPASVAEPHPQPI
                     IEPAAPVVDPALILAFERGVVDLASERERVFAETAAQIAELAVLIARRVIGRELSLNP
                     SIVRGLVREGLSALGQHDRAQVRLGPGFESQRERLEADFSAAGTRADVRFDPTLEAYG
                     CYVETELGQVDESIESRLETLLLALKPDPENS"
     gene            2514..>3113
                     /locus_tag="VFQ35_01955"
     CDS             2514..>3113
                     /locus_tag="VFQ35_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HET9929415.1"
                     /translation="MLDRLIDRCRSAPVHSPEGVVLDVVGLIVEIGGLRAAVGDTLAL
                     KESFGTTLEVEVVGFKNGRLLTTPLGSLSGVRPGMRVVQTHRGGAVPVASSLLGRVID
                     PFGQPLDGGPRISPERMQPVNAAPPPAFARRPIDHAFRTGVRAIDGLLPLGVGQRMGI
                     FAGAGVGKSTLLGMICRSSQAEVNVVGLVGERGRELNDFV"
BASE COUNT          490 a         1000 c         1114 g          509 t
ORIGIN      
        1 tcgctggcgt gccgggcgcg cgcaccaatc gtcccgatgc cgaggacggc aaagccgcga
       61 cggcgacgcc cgcggctggg ggcgggacga tgcgccgtag ccatacgcga aactgggagg
      121 tcgagcgcgt cacccagaag acgctgactc ctcccggcga actgcatcgg ttgagcgtcg
      181 cggtgctctt gaatggtcgc tgggagcagc gcgggccaaa gtccgtgttc gtgccgcgca
      241 gcccggaaga agtgaaggtc ctcgaagagg tcgtgaagcg cgcggtgggc ttcaacgcgg
      301 agcgcggtga caacgttctc gtacaagccg cgcagttctc gcgagtcgag gagcccacgg
      361 ttgctgccga gccgcgttgg gtcgtcgcga cgaagacgta ctggccagtc gccgccggtg
      421 tcgcagcgct gctcggaatt gcgaccgtgg tcttggtgtg gcgtcgctcg agcaagcaag
      481 cggcgaaggc gctggagctc aaggccgcgt cgtcgacggc ggcgctcaac ggagtcgctg
      541 gtgcgcctga gctcggcgct ggggtgactc cggcagagct cccggctgca ccgggcatga
      601 gcttcgaaga catccgcgcg caagcgctcg aactcgcggc taaagaccct gccaccgcgg
      661 ccgtcgtgct ccgtcagtgg ctcgggtccg ctccggctgc cgcggccgca ccgtagctag
      721 caatcgctcg agtcgcacgg cgacgctcgc cagtatacgg cacgccgctc gcatgacatg
      781 gaggtcgtga cgacgatgga tctctccgga ccgcagaagg cagtgctgat gttgctctcg
      841 atggacgagg cagcagcgac tccaattctc gccgagctcg acgccgaaga ggtgaagcgc
      901 ttacgcgaag ccgccgccgg tctgcgctcg gtgcccacca acgcgctcga aggggtgtac
      961 acggagttca ttcagcgctc gcaggaggcg gtggcggtgc cggggggtgg cgtgccctac
     1021 ttgcggcgcc tggccgggcg cgcgctcggc gaacaagtgg ctcaagagat ctttctcgag
     1081 ggcgtcgagg gtccgatgga gcggctcgcc aacgccgacc cgagcgcgct cgcctccgtg
     1141 ctcgagcacg agcatccgca gctcatcgcg gcggtggtct cacagctcga caacgagcgc
     1201 gccgcgcgcg tgctcgaaca gctgccggtc gcgatgcgtc cggccgtact cgagcgcctc
     1261 ggcaacatgc gcgaaatccc gggggcgctg ctggaagaag tggctcgtgc catcagtgcg
     1321 gagctgccga acagcaatcc cgaagcggtt cgctccgtgg acggcgtcgc gcgttcggcc
     1381 gcgttggtgc ggcggatgga ccgccaagtg ggcgaagcgc tgctcggaac gctcgaagaa
     1441 ggcaactcgg agctcgcagt cgagattcgg cgcgcgatgt acaccttcga ggatctcggc
     1501 gcgctcgacg cgcgcgcgct gcgctcgctg ctcgaggcgg tgccggtcga aaagctcacg
     1561 gtggccctca agacggcctc cgattcactg aagtctcacg tctttaccag catgtcgaag
     1621 cgcgccgcag agcgcattcg ggaggatatg gagtccatgg gcggcgttcg cttggccgaa
     1681 gtcgaagccg cgcagcgcga aatcgtcgag gtcgcgttgc ggctcgacgc agaaggcacc
     1741 atctccctcg agaacgacaa tgcgatggtt tgatccttcg tatccgcacg gcgcgcaggc
     1801 ggcgcgtttc gccgacatca gcgctggcaa gcgcaagatc ccgtcgtggg ttccgagccg
     1861 tccggaggga ccccggcgca acgagtcctc gctgcggccg ccgaagttac cgagcgagtt
     1921 cgtggacgcc gtccgccaag agctggacca agagtacgcc ccgcgcatgc gagcgagcat
     1981 ggtccccgac gcgatccagg cgcgcctctc ggagcgcccg gccgcggcgc ccgcgagcgt
     2041 agccgagcct cacccgcagc cgatcatcga gcccgcggct ccggtagtgg atccggcgct
     2101 gatcctggcc ttcgagcgcg gggtggtcga cctcgcgagc gagcgagagc gggtgttcgc
     2161 ggagaccgca gcgcaaatcg cggagcttgc agtgttgatc gcgcggcgcg tgatcgggcg
     2221 cgaactgagc ctcaatccga gcatcgtgcg cgggttggtg cgcgaaggct tgtcggcgct
     2281 cggtcagcac gatcgggccc aggtgcggct cgggccgggc ttcgagtcgc agcgagaacg
     2341 tctcgaagcc gacttttcgg cggccgggac tcgcgccgac gtgcgcttcg atccgaccct
     2401 cgaagcgtac ggttgctacg tcgagaccga actcggtcaa gtcgacgaat cgatcgagtc
     2461 tcgcctcgag acgctgctct tggccctgaa gcccgacccg gaaaactcgt agcatgttgg
     2521 accgtctgat cgatcggtgt cgcagcgcgc ccgttcacag ccccgagggc gtggtgctcg
     2581 acgtcgtggg tctcatcgtc gagattggcg gtttgcgcgc cgccgtgggc gatacgctgg
     2641 ccttgaagga atcgttcggt acgacgctcg aggtcgaggt cgtcggcttc aagaacgggc
     2701 gattgctgac gactccgctc ggatcgctgt cgggtgtgcg acctgggatg cgagtcgtac
     2761 agactcatcg tggcggcgcg gtgccggtgg cgtcgagcct gctcggtcgc gtgatcgatc
     2821 ctttcgggca gcccctcgat ggagggccgc gcatttcccc cgagcgcatg cagccggtca
     2881 atgccgcgcc accgccggcg tttgcgcggc gtccaatcga tcacgccttc cgcaccggcg
     2941 tgcgcgcaat cgacggtctc ctgccgctcg gcgttggaca gcgcatgggt attttcgcgg
     3001 gtgctggcgt cggcaagagc accttgctcg ggatgatttg ccgctcctcg caggcggagg
     3061 tgaacgtcgt ggggctggtc ggagagcgtg gtcgcgagct caacgacttc gtg
//
LOCUS       DASTKS010000091         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_460768, whole genome shotgun sequence.
ACCESSION   DASTKS010000091 DASTKS010000000
VERSION     DASTKS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460768"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..171
                     /locus_tag="VFQ35_01960"
     CDS             10..171
                     /locus_tag="VFQ35_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET9929416.1"
                     /translation="MVDELPLAQSTVSQHLKILKDSGLIRGDVDGPRVCYCIEPRALR
                     RLKALTASL"
     gene            232..618
                     /gene="arsD"
                     /locus_tag="VFQ35_01965"
     CDS             232..618
                     /gene="arsD"
                     /locus_tag="VFQ35_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HET9929417.1"
                     /translation="MAVVRVFDPPMCCATGVCGPGVDPELARFSADLEWLKRQGIVVE
                     RFNLAQQPGAFVENALVKQALTERGNACLPLVLVDERVAVEGMYPSRDTLAALAGVTV
                     RKLMITGDGGCCGPASADPDKKTSCC"
     gene            629..2383
                     /gene="arsA"
                     /locus_tag="VFQ35_01970"
     CDS             629..2383
                     /gene="arsA"
                     /locus_tag="VFQ35_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HET9929418.1"
                     /translation="MIPFASAPRFLLFTGKGGVGKTSVACATAVALADAGKRVLLVTT
                     DPASNLSEVLQTEIGTKATPVVGTRGLAALRVDPVEAAAAYRERMVGPYRGVLPDASV
                     RSIEEQLSGACTVEIAAFDEFAKLIGDRGLALEYDHVVLDTAPTGHTLRLLELPAAWS
                     EHIVQNPGGVSCVGPLSGLTSQRTLYESARRALADEAQTQIVLVSRLETEALKEAERA
                     RRELAALGMRRLHLVLNGVFQARDLADPIAVALEQRQTAARESLSPALSALPRTELQL
                     MSRSLLGIARLRSFSSEPEKAVELAESVPGGDFPSLSSLLPELERSGSGVILLLGKGG
                     VGKTSIAIRIASALAERGHAVQLTTTDPAHDPEAFVALEEAGVRVGYIDRHREVRAYV
                     EETLATAGAGLDDAGRALMAEELAAPCTEEIAVFRAFAREVDAGQRGFVVIDTAPTGH
                     TLLLLDAAAAYHREVLRSPGGVSEEVRNLLPRLRDPEFAKLVLVTLPEATPVHEASQL
                     KDDLGRASMRPVAWVVNQALSPLRVSDPILRARQAAEAVYISEVRALAERMAVVPFEP
                     LTRGAASVAPASPSRPHE"
BASE COUNT          402 a          807 c          849 g          434 t
ORIGIN      
        1 tgcggagaga tcgtcgacga gctcccgctg gcccagtcga ccgtttccca gcacctgaaa
       61 atcttgaagg atagcgggct aattcgcggc gacgtagacg gaccgcgagt ctgctactgc
      121 atcgagccgc gcgcgctgcg gcgattgaag gcgttgaccg ctagtctctg agagcccgtc
      181 gctctcgcct gcatcgagct ctgcatcgag cccgaactcg aggaaaagaa catggccgta
      241 gttagagtgt tcgacccacc catgtgttgc gccaccggcg tctgcggacc cggcgtcgac
      301 cccgaactcg cccgcttcag cgccgatctc gagtggctga agcgtcaagg catcgtcgtg
      361 gaacgattca atctcgctca acaacccggc gcgttcgtgg agaatgcgct cgtgaagcaa
      421 gccctcaccg agcgaggcaa cgcttgcttg ccgctcgtgc tggtggacga gcgtgtcgcg
      481 gtcgaaggaa tgtacccttc gcgagatact ttggccgcgc tcgccggcgt tacggtgcgg
      541 aagttgatga taacgggcga cggcggttgt tgcgggccgg ccagcgccga tcccgacaag
      601 aagacgagct gctgctgagg agcgaaccat gattcccttc gcaagcgctc ctcgcttttt
      661 gctgttcacg ggcaaaggcg gcgtcggaaa gaccagcgtg gcctgcgcca ctgcggtcgc
      721 gctcgccgac gcgggtaagc gggtgctctt ggtcacgacg gatcccgcgt cgaacttgag
      781 tgaggtgctg cagacggaga tcggcacgaa ggcgacgccg gtcgttggca cgcgcgggct
      841 cgcggccctt cgagtggacc cggtcgaagc tgcggcggcg tatcgcgagc gaatggtcgg
      901 gccctatcgc ggcgtcttgc cggacgcttc agttcgcagc atcgaggagc agctttccgg
      961 cgcctgcacg gtggagatag ctgcgttcga cgagttcgcg aagctcatcg gcgaccgcgg
     1021 actcgcgctc gaatacgacc acgtggtgct cgatacggct cccactggcc acacgctgcg
     1081 gttgctcgag ctgccagcgg cttggagcga gcacatcgtc caaaatccgg gcggggtctc
     1141 gtgcgtggga cctctgtctg gcctcacttc gcagcgcacg ctctacgaat ccgcgcggcg
     1201 cgcgctggcc gatgaagcgc agacgcaaat cgtgctggtc tcgcggctcg agaccgaggc
     1261 gctgaaggag gccgaacggg cgcggcgtga gctcgcggcg ctcgggatgc gacgccttca
     1321 tctcgtgctc aacggcgtgt ttcaggctcg ggatctggcg gatccgatcg cggtcgcgct
     1381 cgagcaacgc cagacggcag cgcgcgagtc gctcagcccc gcgctctcgg ctttgccgcg
     1441 caccgagctc cagttgatga gtcggtcgtt gctcggcatc gcgcgcctgc gctccttctc
     1501 gagcgaaccc gagaaagccg tggagctcgc ggagtcggtc ccaggcggcg acttcccgag
     1561 cctttcgtcg ctgctgcccg agctcgagcg ctctggctcg ggggtgatcc tcctgctcgg
     1621 caagggcggc gtcggaaaga cgagcatcgc cattcggatt gcgagcgctc tggcagagcg
     1681 cggtcacgcc gtgcagctca cgaccaccga ccctgcccac gatcctgagg cgttcgttgc
     1741 tctcgaagaa gctggcgttc gcgtcggcta catcgatcgc caccgcgagg tgcgcgctta
     1801 cgtggaagaa acgttggcca ccgctggcgc ggggctcgat gacgcgggtc gcgcgctcat
     1861 ggcagaggag ctcgctgcgc cgtgcaccga ggagatcgcg gtcttccgag cttttgcccg
     1921 cgaggtagac gctgggcagc gcggcttcgt ggtgatcgac acggcgccga cgggacacac
     1981 tttgttgctg ctcgacgccg cggccgccta tcaccgcgag gttctgcgtt cgccgggcgg
     2041 tgtttcggaa gaagtgcgaa acctgctgcc tcgcttgcgc gaccccgaat tcgcgaagct
     2101 cgtgctcgtg acgctaccgg aggccacgcc tgtgcacgag gcgagccagc tcaaggacga
     2161 cctcgggcgc gcctccatgc gtcccgtcgc ctgggtagtc aatcaagcgc tctcgccgct
     2221 gcgtgtcagc gatccaatcc tccgcgcccg ccaggccgcc gaagccgtgt acatctcgga
     2281 ggtacgagcg ctcgcggagc ggatggccgt cgtcccattc gagccgttga cgcgaggtgc
     2341 cgcgagcgta gcaccggcgt cgccgagtcg gcctcatgag tagccaaggt cgaacgacgc
     2401 cgcaaaagct cgtcgccgaa gcgttgagca cggcgctact cctcgcgatc gtcgtcggct
     2461 cgggtgtaat ggccgaagag ctagcgggcg ga
//
LOCUS       DASTKS010000092         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_906176, whole genome shotgun sequence.
ACCESSION   DASTKS010000092 DASTKS010000000
VERSION     DASTKS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906176"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFQ35_01975"
     CDS             <1..764
                     /locus_tag="VFQ35_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929419.1"
                     /translation="PESELFVSGAAWDARCSTNRTSCASGYDVLAMGSLLEWTAGDVA
                     SEGSARRAGVRGAPANAPGPLHRCAARRALDPDNRSDEVGFRCCAGAPNATGMVEPKL
                     DVTFKKTRIQADELAKLFARDPATQELAKDIRFFKDPDSAETVVSRGPGDRKGFTFTV
                     APLLWNPVAGAEFLLVTGRSGENTSFVAAFYVISDNEYRLASSFVLRSEPGPIAFAYD
                     AGIRPRVHFSTCWGCPGETGKILFRKPERAVILQP"
     gene            complement(831..1541)
                     /locus_tag="VFQ35_01980"
     CDS             complement(831..1541)
                     /locus_tag="VFQ35_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9929420.1"
                     /translation="MAEADAKASVGALLALVAHDLRNPLSALHSNVGFLESSLGPKEQ
                     DSIDALADVVASCSSLKHIIDNLELLGLSVLDEPPHFERFASSLWELAVEAVQRLEPI
                     AKSYGARVSLEGDRFAAPRVVVQRDMFVRALGNLLFNAIQSGGSQGQVVVVVSGNGSR
                     GIVTVSDSGPVLATALRGSAFTASGQLDCKGDPRGRYSRGLGLFAAATAARFGGAEVR
                     GVDGPNGKNSFEITAPLA"
     gene            complement(1550..2371)
                     /locus_tag="VFQ35_01985"
     CDS             complement(1550..2371)
                     /locus_tag="VFQ35_01985"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9929421.1"
                     /translation="MRQRAGHGRTLFLLCDGIVCDGYIYKYLWDDLSEFGAVAHWNYR
                     GHGRSALPSNPNCIRVIDHARDLDAVRRHLGDPPVVLVGHSFGTQVALEGYRLRPEGV
                     RALMLLSGSFGRVTYTFKGTDVLANVLPQIIDFVERHPRLARGLWSRIPVSLGLALAR
                     LSGDVNLESVRADDMRPYFEHAAHVDFEMFLRMLREAGEHSAEDLLPHVDVPALIVTG
                     EKDSFTPPSLSQAMAQAMPRAEFALVSGGTHILPLEEHDQLKQIIAAFLARFPVE"
     gene            complement(2427..2648)
                     /locus_tag="VFQ35_01990"
     CDS             complement(2427..2648)
                     /locus_tag="VFQ35_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HET9929422.1"
                     /translation="MAENDSNKAAGSDKRKQSLYFPESMLQEIKDEAARLDRSLSWVV
                     QRAWKMARLEIKKIPSVNDVEDDGEEAAG"
     gene            2872..>3611
                     /locus_tag="VFQ35_01995"
     CDS             2872..>3611
                     /locus_tag="VFQ35_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9929423.1"
                     /translation="MRRSPRALHVIAGTALALITLHADRAARATTQRENPYALLDQLG
                     RVLSFVENEYVDPVSHQTLLEGALEGMVAKLDPHSAYLPPEDYSIFQSDTEGRFGGVG
                     VEVDFGEDWVTVIAPIEGSPAERAGVRPGDRIVAIDHVSVRGKRPAELVRQMRGTPGG
                     KVLLTISRPGSDKYLYFTLTREVINVASVAGKMLSEGVGYLRIKSFQMGTHTELLSEF
                     GELRKKAKGTPAGIILDLRNNPGGLVNEA"
BASE COUNT          701 a         1168 c         1120 g          622 t
ORIGIN      
        1 gaccggagag tgagctcttc gtgagcggcg cggcgtggga tgcacgctgc agcaccaatc
       61 ggactagctg cgcttcggga tacgacgtcc tcgcgatggg ctcactgctc gagtggacgg
      121 ccggcgacgt cgcgagcgaa gggtccgcac ggcgcgcggg cgtgcgcgga gctccagcca
      181 atgcgcccgg gccgcttcac cgatgcgcag cacgacgcgc gctcgacccc gataaccgtt
      241 ccgatgaagt cggcttccgt tgctgcgcag gagccccgaa cgcgaccggc atggtcgagc
      301 ctaagctcga cgtcacattc aagaagacgc gtatccaggc cgatgagttg gcgaagctgt
      361 tcgcgcggga tccggcgacg caagagctcg cgaaggatat ccgcttcttc aaagaccccg
      421 actccgcgga aacggtcgtt tcgcggggac ccggcgaccg caaaggcttc acgttcaccg
      481 tcgcgccgct gctctggaac ccggtcgcag gtgcggagtt tttgctggtg acgggccgct
      541 cgggcgagaa cacgtcgttc gtcgcggcgt tctacgtcat ttcggacaac gagtaccgac
      601 tcgcctcgag cttcgttctg cgcagcgaac caggcccgat cgcgtttgcc tacgacgccg
      661 ggatcaggcc tcgggtgcac ttcagcacgt gctggggctg ccctggcgaa accgggaaaa
      721 tcctgttccg caagcccgag cgcgcggtga ttctgcagcc gtagacaccg ctaacgagcc
      781 cacgattcta gagccgctgc gctcgcacaa aactcggagc gaggctgaag tcaggcgagc
      841 ggagcggtga tctcgaacga gttcttgccg ttcggaccgt cgacgcctcg gacctcggcg
      901 ccgccgaaac gggccgccgt cgccgcggcg aacaagccga gaccgcggct gtagcgacca
      961 cgcggatcgc ctttgcagtc gagctggccg ctcgcagtga acgcgctgcc gcgcagcgcc
     1021 gtggcgagga cggggccgga atcagaaacc gtcacgatgc cgcgcgaacc attcccgctc
     1081 acgacgacca cgacttgccc ctgacttccg ccgctctgaa tcgcgttgaa gagcaggttc
     1141 ccgagagcgc gcacgaacat gtcgcgttgc acgaccaccc gcggcgcagc gaatcggtcg
     1201 ccctcgagcg agacgcgcgc gccgtaactc ttcgcgatgg gctcgaggcg ctgcactgct
     1261 tcgaccgcga gctcccacag agaactcgcg aagcgctcga agtgcggagg ctcgtcgagg
     1321 accgacaacc ccaaaagctc gagattgtcg atgatgtgct tcagggagct gcacgacgcg
     1381 acgacgtcgg cgagcgcgtc gatcgaatct tgctctttcg ggccgagcga gctctccaga
     1441 aaaccgacgt tgctgtgcaa cgcggagaga ggattgcgca ggtcgtgcgc aaccagagcg
     1501 agcagcgctc cgacgctggc tttcgcgtct gcttcggcca ttccgtctac tattccacgg
     1561 ggaaccgcgc gagaaaagcc gcgatgatct gctttagttg atcgtgctct tcgaggggca
     1621 agatgtgggt gcccccagac accaaggcga actccgcgcg tggcatggct tgcgccattg
     1681 cctgcgagag cgacggcggg gtgaacgagt ccttttcgcc cgtgacgatc agggccggaa
     1741 cgtcgacgtg cggcagcaga tcttctgcgg aatgttcgcc agcttcgcga agcatccgca
     1801 aaaacatttc gaagtcgacg tgggccgcgt gctcgaaata cggccgcatg tcgtcggcgc
     1861 gcaccgactc gaggttcacg tcgccgctca gacgagccaa cgcaagcccg aggctcaccg
     1921 gtatacgtga ccagaggcca cgcgctagcc gcgggtgccg ctccacgaaa tcgatgatct
     1981 gtggaagcac gttcgcgagc acatcagtgc ccttgaacgt gtaggtcacc ctaccgaagc
     2041 tgcccgaaag cagcatcaac gcgcgcaccc cttcgggacg caaccgatag ccttctagag
     2101 ctacttgcgt cccgaaagag tgccccacca aaacgacggg cgggtcgccc agatgccgcc
     2161 gaaccgcgtc caaatcgcgt gcatggtcaa tgacgcgaat acagttcggg ttcgacggca
     2221 gcgcactgcg accgtgtccg cggtagttcc agtgagctac cgcgccaaac tccgacaggt
     2281 catcccataa atacttatag atgtacccgt cgcagactat cccatcgcag agcaggaaca
     2341 gagtcctacc gtgcccggca cgctgcctca cataaaggcg cgtgccgtcg ggccccgatg
     2401 caaacgactc gaggcccgaa gctgcctcag ccggcggctt cctcgccgtc gtcttcaacg
     2461 tcgttcacgc tcgggatctt cttgatctcg agccgcgcca tcttccatgc gcgttgcacg
     2521 acccaggaca gcgaccggtc gagtcgcgcc gcctcgtcct tgatttcctg aagcatcgat
     2581 tctggaaaat agaggctctg tttgcgcttg tcgcttccag cggctttgtt ggagtcgttt
     2641 tcggccacgc ttacctcgga gcagaaatgg gtggtcggac cctgccgacc ctccgatttg
     2701 ttaatggtta ccttagtagc acccgcagac cctgggaagc aatggggtat tgtggcgaac
     2761 gacatgaccg ttacgcgtgc gcgcttctcc cgcctttccc gaatcgttcc acctcgtgaa
     2821 cccacaacaa tcccgccaaa accgggagat tttggctcga gtcgacggcg gatgcgacgc
     2881 tctccccgag cgctccatgt gatcgcagga actgcactcg cgctcatcac cctacatgcg
     2941 gaccgcgctg cgagggccac gacacagcgg gaaaacccgt atgctttgct cgatcagctc
     3001 ggtcgagtgc tcagcttcgt cgagaacgaa tacgtcgatc cggtgagtca ccaaacgctg
     3061 ctcgaaggcg cgctcgaggg catggtggcg aaactcgacc cgcattcggc ctacttgccg
     3121 cccgaggatt actcgatttt ccagagcgat accgaagggc gtttcggcgg cgtcggcgtg
     3181 gaggtcgatt tcggagagga ctgggtcacc gtgatcgcgc cgatcgaagg atccccagca
     3241 gaacgcgcag gagttcggcc aggtgaccgc atcgtcgcga tcgatcacgt gagtgtgcga
     3301 ggaaaacgcc ccgcagagtt ggtgcgtcag atgcgtggca cgcccggcgg caaggtgctg
     3361 ctcacgatca gccggcccgg cagcgacaag tacctctact tcacgctcac ccgcgaggtc
     3421 atcaacgtgg cctccgtcgc gggcaagatg ctgagcgaag gagtcgggta cctccggatc
     3481 aagtcgtttc aaatgggaac gcacaccgag ctgctctccg agttcggcga gctccggaag
     3541 aaggccaaag gaacgcccgc cggcatcatc ttggacctcc gcaataaccc cggtggctta
     3601 gtcaacgagg c
//
LOCUS       DASTKS010000093         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_14974,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000093 DASTKS010000000
VERSION     DASTKS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14974"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1589)
                     /locus_tag="VFQ35_02000"
     CDS             complement(108..1589)
                     /locus_tag="VFQ35_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9929424.1"
                     /translation="MRIAFGCALAALTAVLATLSFAPYDVWWVVWFAFVPMIVAQYRV
                     LPARWSSLGPAIGVGGFVFGYVGGAFPAHAAWYMKALPLVFFIVLFASARGGRVRQDR
                     VGYRLLPIAAATAWVVVEFVRIPFIATWGYFGYALYEQSWLLQPVRVFGIFGLDLLIA
                     LVNYALALGIMAALDRRQSFEAPVRIESRQALGWCAIALGATAGWACASLMWPRAALP
                     TVRVAALQPGVRPFELGKTNEERDRRMLERLSAQTRMAAGRAARVVVWPEAALSKDPA
                     LAYRAELGELARETGAYLVVGYGVVEPHGFRNEVVTVTPSGEIVGRYGKNHPVSFLGG
                     SSIWRGVYPTVDTPYGKIGSVICYDMDFTDTPRELALRGAKLVAVPSSDWRAIASKHY
                     TFAVFRALETGAAFAKSEYSRDSAIVDPYGRIVASVVTPNGSDDVLVADVSLRSGVPL
                     AALWGDWVAWLCVIAVAVRAIRSVAHRIGRTRSRDLEAHLGQG"
     gene            complement(1782..4694)
                     /locus_tag="VFQ35_02005"
     CDS             complement(1782..4694)
                     /locus_tag="VFQ35_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9929425.1"
                     /translation="MQGAGRRRAVGVLLNYTSFFSEGIEGRLRRAFDVECTRRDLDLY
                     LIYGRAIEEPDPSCAAYNAVFDLVHPDRIDALILVSNLLAGSCGSARLDEYAQRFSHL
                     PRCSLGMVLESMPSVDVDNRPGMAALVAHLIEAHGHRRFAFMGGQENHPHSRLRLEVC
                     RSVLAQHGLSLDPALVVYGDFMTHAAEVAMDELLAARADFDVVVAANDAMALGVVASL
                     RKHGIGVPEQCAVTGYDDLIHARLGNPPLTSVAQPLEALALHALDSVEAQLAGKQVAR
                     ATLLTGELCLRDSCGCSARATFAAAEATRPASTALEHLRRSFTHLHAQVERLLGEPRS
                     AERDTATELLRGLEAELSAERPALVQVMRGLLSSVRDDPEHFRAWYEVVEFLAGEFQP
                     FSSARLDAALRGVQRELVLGLTASHLEQQLAMDFTYVQLLGSEDNVFGALKLGGLQDS
                     LRRHLPTAGIGTVALSEYVAGESGELQTLVYFVDGEGDSSRRERFPAHWLFPPSAKPK
                     GRKTFVVMPLVFESHGLGMSVLEYAPGPIPYHLLRDQLSAAVGTYKLQKEIEHQSALR
                     ERSVQERLAAQQRMESLSLLAGGVAHDLNNVLGPLVALPDLMLPELEAASTNSGTIDE
                     LRKDLELIKVAGLRASQTIKDLLTLSRQGRTAKAALDLNRLVLECARDGATQELRVAN
                     HEVRVHTTIAAEPLIMEASEAQIARAILNLLQNAVDAIDGPGEVHLRSYALDLVAAYD
                     AHETIPPGSYVVFEIRDTGRGLPIADRRRIFEPFFSTKPLGQRSGTGLGLAIVHGVVK
                     EHGGFIDVVSQVGDGTSFSLYFPRSAREPVSARVPLAQRAGGERILVVDDEPIQLRTC
                     RRVLTHFGYEVEITSSGSEACERFARVQAGTCKGYDLVLLDVQLAAGEDGLEIYERIR
                     ELSPNQKAVLVSGHAPTHRIKAALERGIPWLQKPYTADALADTVRSALNGTIETRC"
     gene            complement(5058..>5627)
                     /locus_tag="VFQ35_02010"
     CDS             complement(5058..>5627)
                     /locus_tag="VFQ35_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929426.1"
                     /translation="AWRVREALCDLTRACCETAGFFDDPFDHCLAQSPGDADQYRASI
                     TAGRLSIDDAALRSCADHIRATYLGCDSLLSVLDTFATFSDCVDGLAGHVPIGAACTL
                     NEECVGAGTGRVKCVDGFCADGVTNISDDAACTLDDHCGAKSYCDGTCRPKKAVGANC
                     TSGHECTSLHCNQLTRTCATLPDQAECEG"
BASE COUNT         1024 a         1832 c         1847 g          924 t
ORIGIN      
        1 agttccggtc ctccttaccc gtcccaatgc cggtggtttt gatactgcct ttcgtcgtgt
       61 tggcggcggg tctcgcattg cgactcgcgc actgactcga cccgttgcta cccctggccg
      121 agatgtgctt cgaggtcgcg tgacctggtg cggccaatcc tgtgcgcgac gcttcgaatg
      181 gcgcgaaccg cgactgcgat gacgcaaagc caggccaccc agtcgcccca gagcgccgcg
      241 agaggaacac cgcttcgaag cgagacatcc gcgacgagca cgtcatcgct tccgttcggc
      301 gtcaccacgc tcgcgacgat acgaccgtat ggatcgacga tcgccgaatc gcggctgtac
      361 tctgattttg cgaaggccgc tcccgtctcg agcgcgcgga agacggcgaa ggtgtagtgc
      421 ttcgacgcga tcgcgcgcca atcggaggac ggcactgcga cgagcttggc gcctcggagc
      481 gcgagctccc gcggcgtatc ggtgaagtcc atgtcgtaac aaatgacgga accgattttt
      541 ccgtacggcg tatccaccgt cggataaacc ccgcgccaga tgctggatcc gccgaggaag
      601 ctcacggggt gattctttcc gtagcgaccg acgatctcgc cgctcggagt cacggtgacc
      661 acttcgttgc ggaagccgtg aggctcgacg acgccgtaac cgacgaccaa gtaagcgccc
      721 gtctcgcgcg ccagttcgcc gagctcggct cgatacgcga gcgctggatc cttcgacagc
      781 gccgcttccg gccagaccac gacccgcgcc gcccgccccg ccgccatgcg cgtttgcgcc
      841 gaaagccgtt cgagcatgcg tcgatcgcgc tcctcgttcg tcttgccgag ctcgaacggc
      901 cgcactcccg gctggagcgc ggcgacgcgc accgtcggaa gcgccgcgcg gggccacatg
      961 aggctcgcgc aggcccagcc cgcggtggcg ccgagcgcga tcgcgcacca cccaagcgcc
     1021 tgtcgtgatt cgatacgaac tggcgcctcg aaactttggc gccgatcgag cgccgccatg
     1081 atgcccaagg cgagcgcata gttcacgagc gcgatcagga ggtcgagccc gaagatgccg
     1141 aacacgcgca ccggttggag caaccacgac tgctcgtaga gcgcgtagcc aaagtagccc
     1201 cacgtcgcga tgaacgggat gcgcacgaat tcgacgacaa cccacgcggt cgcagccgcg
     1261 atcgggagta gtcgataccc gacgcgatct tggcgcactc gcccgccgcg agcgctcgcg
     1321 aagagcacga tgaagaagac gagcgggagc gccttcatgt accaagcggc gtgagcggga
     1381 aatgcgccac cgacgtaccc gaatacgaag ccaccaactc cgatggcggg gccgagcgac
     1441 gaccaccgcg ccggaagcac gcgatattgg gccacgatca tcggcacgaa cgcgaaccag
     1501 acgacccacc acacgtcgta cggtgcgaag ctcagcgtcg cgagtaccgc agtgagcgcg
     1561 gcgagagcgc agccaaaagc gatcctcatg tgtgctttcg ccgatggggt tcagcgttcg
     1621 cgcgaccggg cagggtttcc cgttcgggct tcggggccat ggctgcggct tttatagtga
     1681 ctatttagtc gaggagcaac gagccaaccg ggtgcgaagt tacccggttc ggccgttttt
     1741 acgccggaag ggcacacaag cagaccgtgt gccgagccga ctcagcagcg ggtctcgatc
     1801 gtgccgttca aggcggagcg cacggtgtct gccaacgcgt cggcggtgta gggcttctgc
     1861 agccacggga tgccgcgttc gagagcggcc ttgatgcgat gcgtgggcgc gtgcccgctt
     1921 accaggaccg ccttctggtt gggcgatagt tcccgaatcc gttcgtaaat ttcgagcccg
     1981 tcttcgcccg cagccagctg cacgtcgagc agcaccaagt cgtacccttt gcaagtacca
     2041 gcctgcacgc gcgcgaagcg ttcgcaagcc tcgctgccgc tcgaggtgat ctcgacctcg
     2101 tacccaaagt gggtgagcac acgccggcaa gtgcgcagtt gaatcggctc gtcatccacg
     2161 accagaatgc gttcgcctcc cgcccgctgc gcgagaggca cccgggcact cacgggttcg
     2221 cgcgcgctgc gggggaaata gaggctaaag ctggtgccat ctccgacttg gctgacgacg
     2281 tcgatgaacc caccgtgttc tttcacgacc ccgtgtacga tcgcgagccc aaggcccgtt
     2341 cccgagcgct gtccgagggg cttggtgctg aagaagggct cgaagatgcg tcgtctgtcg
     2401 gcgatcggga gcccgcgccc cgtgtcgcgg atctcgaaga ccacgtagct gcccggtgga
     2461 atcgtctcgt gtgcgtcgta agcggcgacg agatcgagag cataggagcg caggtgcacc
     2521 tcgccgggtc cgtcgatggc gtcgacggca ttctggagca agttgaggat cgcgcgcgcg
     2581 atctgcgctt cggaagcctc catgatcaac ggttcggccg cgattgtcgt gtgcacgcgg
     2641 acttcgtggt ttgcgacgcg gagctcttga gtcgcgccgt cgcgggcgca ctcgagaacc
     2701 agacggttga ggtcgagcgc agccttggcg gttcggcctt gccggctcaa cgtcagaagg
     2761 tccttgatcg tctgggaagc acgcaacccc gcgaccttga tgagttcgag gtctttgcga
     2821 agctcgtcga tggttcctga attggtcgag gccgcttcga gctcgggcag catcaggtcg
     2881 ggtaaagcca cgaggggtcc cagcacgttg ttcaagtcgt gggccacgcc gccggcgagc
     2941 aggctcaagg actccatgcg ctgctgcgcc gcgagacgtt cctggacgct gcgttcgcgc
     3001 agcgccgact ggtgctcgat ttccttttgc agtttatagg ttccgacggc agcgctgagt
     3061 tggtcgcgca acaagtggta gggaatcgga ccgggcgcgt actcgagcac gctcatgccg
     3121 aggccatgcg attcgaagac caacggcatc acgacgaagg tcttgcggcc tttcggcttt
     3181 gcgctcgggg gaaacaacca gtgagcggga aagcgctcgc gccgcgacga gtcgccttcg
     3241 ccgtcgacga agtagaccaa ggtttgcagc tcacctgatt cgcccgcgac gtactcggag
     3301 agcgccaccg tgccgatgcc tgcggttggc aagtgacgtc ggagcgagtc ctgcaaacct
     3361 ccgagtttga gcgcgccaaa cacgttgtct tcactgccga gcagttgcac gtacgtgaag
     3421 tccattgcca gctgttgctc gagatggctc gccgtcagtc ccaacacgag ctcgcgctgc
     3481 acgccgcgca atgccgcatc gagccgtgcc gacgagaaag gctggaactc gcctgccagg
     3541 aactccacca cctcgtacca ggctcgaaag tgttcggggt cgtcacgaac cgagctcaac
     3601 aatccacgca tgacctgcac gagcgcgggt cgctccgcgg agagctcagc ctccaggccg
     3661 cgcaagagct ccgtggccgt atcgcgctcc gccgagcggg gctcgccgag tagtcgctcg
     3721 acctgggcgt gaaggtgcgt gaaggagcgt ctcaggtgtt cgagcgctgt cgatgccggg
     3781 cgcgtggcct cggcggccgc gaacgtcgcg cgcgccgagc agccgcacga atcgcgcaga
     3841 cagagctcgc ccgtcagcag cgtcgcgcgc gcaacctgtt tgccagcgag ctgggcctcg
     3901 acggagtcca gtgcgtgcag ggcgagcgcc tcgagcggct gggccacgct cgtgagcggc
     3961 ggattgccga gccgcgcgtg gatcaagtcg tcgtagcccg tgacggcaca ctgctcggga
     4021 actccgatgc cgtgcttgcg gagcgaagcg acgacgccga gtgccatggc atcgtttgcc
     4081 gcgacgacca catcgaagtc ggcgcgcgcg gcgagtagtt cgtccatcgc cacctcggcg
     4141 gcgtgggtca tgaagtcgcc atacacgacc agcgcgggat cgagggagag tccgtgctgc
     4201 gcgagcacgc tgcggcatac ctcgagacgc aggcgtgagt gggggtgatt ctcctgcccc
     4261 cccatgaagg cgaagcgccg gtggccgtgc gcctcgatca agtgcgccac cagcgccgcc
     4321 atgccgggcc gattgtcgac gtcgacgctg ggcatgcttt cgagaaccat ccccagactg
     4381 cagcgcggca gatgggagaa gcgctgcgca tactcgtcca ggcgcgcgct cccacaactg
     4441 cccgcgagta ggttcgagac caagatcaag gcatcgatgc gatccggatg cacgagatcg
     4501 aacaccgcgt tgtaggcggc gcagctcggg tctggctcct cgatcgcgcg gccgtagatc
     4561 aaatacaaat cgagatcacg ccgcgtacac tcgacatcga aggcgcgccg caagcgccct
     4621 tcgataccct cgctgaagaa gctcgtgtag ttcagcaaca ccccgaccgc ccgacgccgg
     4681 cccgccccct gcatcacggc atctccgcgc tctgcgtcgg acgcgcactg ggaccgtgcc
     4741 ccctacccag gcctacttgg cccgagcacc acagcgaggt ctctcgtacg cagcacaccc
     4801 gggtctggag taagacctgg tgatcccccg aactcgggac ccgagcgcgc atcgtcggtg
     4861 agcgtcgaag cggctgcgag accatcgcct gacagcaacg accgcgcgac cgcaggggtt
     4921 tacccgtcgg gtagaagaac tacacggggg actactggac gaaagcctcg aacaaccgtg
     4981 ctcgcgtcgg gtaggttcgc acatcggcgc gcaggctttg cgcgtcgagt tcgagccagg
     5041 cagtgcggtc gcgaggctca gccctcgcac tcagcctgat ccggcagcgt cgcacaggtt
     5101 cgggtaagct gattgcagtg gaggctcgta cactcgtggc cgctcgtgca attggcgccg
     5161 accgctttct tgggccggca agtcccgtcg cagtaggact tcgctccgca gtgatcgtcg
     5221 agcgtgcaag ccgcgtcgtc ggagatgttg gtgacgccgt ctgcacagaa gccgtcgacg
     5281 cacttaacgc ggccggtacc ggcgccgacg cactcctcgt tgagggtaca ggccgcgccg
     5341 atcggaacgt gtccggcgag gccgtcaacg cagtcggaga aggtcgcgaa cgtgtcgaga
     5401 accgagagca atgaatcgca gccgagatac gttgcgcgaa tgtgatcggc acaggatcgg
     5461 agcgcggcgt cgtcgatcga taagcgaccc gcggtgatac tcgcgcgata ctggtcggcg
     5521 tcgccgggag attgcgctag gcaatgatca aacgggtcgt cgaagaagcc cgccgtttcg
     5581 cagcaagcgc gggtcaagtc gcacagcgcc tcccggacgc gccacgc
//
LOCUS       DASTKS010000094         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_236710, whole genome shotgun sequence.
ACCESSION   DASTKS010000094 DASTKS010000000
VERSION     DASTKS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236710"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFQ35_02015"
     CDS             complement(<1..1176)
                     /locus_tag="VFQ35_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9929427.1"
                     /translation="MDARQFEAVLDCVFDCVVTLDGAGKIGATNLATQETFGYTREEL
                     QGKDLKELVHGGVDDLLTGTQRKTPRRQGYVGCRYDGSLFPLELALTRVDHAGEPLTV
                     VTLRDVSEYTSHTDINEGFDDAPLSRQDELEALVHEKTRDIERERATIELLLSVAYCA
                     NYSTSLREALAATLELLCNHLGWELGHAASFDACSGTFAANELWFGPWPQTLIEAHRT
                     SALATAAVRNRAPVVLTDRTLVDEAPLTLAGMANGLALPILVGNEVVAVLEAYALDPS
                     EAEHSPVALAKLIVAQLGLVAQREEREHELRTAAAVAEAATRQKSEFLSNMSHEFRTP
                     MHAIINYTNLALRGVDRTEPERLKHYLNNVSVSSRRLLAMLNDILDLAKMDSGRFQCE
                     "
     gene            complement(1290..2420)
                     /locus_tag="VFQ35_02020"
     CDS             complement(1290..2420)
                     /locus_tag="VFQ35_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929428.1"
                     /translation="MESIRSARAGLDESSLKPARNPWITVGFRELIAEPTRSVASTAF
                     LFAWLSAGHTAGASPKVSAEAQGHFRNGVELLQSEAPNYQDAYDQFKLAYEKSHSWKV
                     LGNLGLCALKLERDGEALADFEEYLKVGGRNVAKSERAAIERDMLLARGNAANVELVA
                     SSEGEVIDSRAMSTAGAQHYALEFGTTTLLVRAGTHTFVARDKSGRALTWAVTLGPGS
                     RVTHRFDFDVSAKDHEAKQRPPTEPTVRPALQTESAPKGRADSPLRAVGFVALGASVV
                     TIGAGAVFAVFAKGHERSALQRCSSDYVCDPSARPDFETATSQMRVANVLVVSGAVIG
                     VAGLALVLFGGPGQSEARAEHAFRVTPTFGTGSAGLVARGHF"
BASE COUNT          428 a          796 c          794 g          474 t
ORIGIN      
        1 ttcgcattgg aaccggccag agtccatctt ggcgaggtcg aggatgtcgt tcagcatcgc
       61 gagcagtcgg cgggaagaga cggagacgtt gttcaggtag tgtttgaggc gctcgggttc
      121 ggtgcggtcg acgccacgta acgcgaggtt cgtgtagttg atgatcgcgt gcatcggcgt
      181 gcgaaactcg tgcgacatgt tggacaggaa ctcggacttc tggcgcgtcg ccgcctcggc
      241 cacggccgcg gcggtgcgaa gctcgtgttc gcgttcttcg cgctgcgcga cgagtccgag
      301 ctgcgccacg atgagcttcg cgagcgcgac gggcgaatgc tcggcttcac tcggatccag
      361 cgcataggcc tccaacactg caacgacctc gttccccacc aaaatgggta aggcgagccc
      421 gttggccatt cccgcgagcg tcagtggagc ttcgtccacg agcgtgcggt cggtgagcac
      481 gaccggagcg cgatttcgga ccgccgcggt cgcaagggcc gacgtgcggt gcgcctcgat
      541 caatgtctgt ggccatggcc cgaaccacag ctcgttggca gcgaaagtcc cggaacaggc
      601 gtcgaacgac gcggcgtggc cgagctccca gcccaggtga ttgcacagga gctcgagcgt
      661 ggcggcgagc gcttcacgca gcgacgtgga gtagttcgcg cagtacgcga cgctcagcaa
      721 gagctcgatg gtggcccgct cgcgctcgat atcccgggtc ttttcgtgga ccagagcctc
      781 tagctcgtcc tgtctggaaa gcggcgcgtc gtcgaacccc tcatttatgt ctgtatggct
      841 cgtatattca ctcacgtcgc gcagcgtgac gaccgtgagc ggttcgccgg cgtggtctac
      901 gcgcgtgagc gcgagctcga gaggaaacag gctaccgtcg tagcgacatc cgacgtaccc
      961 ttgcctgcgc ggcgtcttcc gctgggtgcc cgttagtagg tcgtcgaccc cgccgtgcac
     1021 taactccttg agatctttgc cttgcagctc ttcgcgcgtg tagccaaagg tctcctgagt
     1081 agcgaggttg gttgcgccga tcttgccggc cccgtccaag gtcacgacgc aatcgaagac
     1141 acagtccaac acagcctcga attgccgtgc gtccatcgtc gcgagcaaaa ggacgacgac
     1201 tgagagggag tgcttaaggt ggcggctctc gtagattgcg ttttctcacg acggagcgac
     1261 cgagtattcc cgtacaaaat ccccgaatct tagaagtgcc ctctcgctac gagaccggcg
     1321 ctgcctgttc cgaaagtcgg tgtaacgcga aaggcgtgct ccgcgcgcgc ctccgactgg
     1381 ccaggaccgc cgaaaagaac caaggcgagg cccgcgacgc cgatgaccgc gccgctcacc
     1441 accaagacgt tcgcgacgcg catttgcgaa gtagcggttt cgaagtcggg gcgcgcgctc
     1501 gggtcacaga cgtagtcgct gctgcagcgc tgcaacgccg aacgttcgtg ccccttcgcg
     1561 aacacggcga atacggcgcc tgcgccaatg gtcaccaccg acgcgccgag cgccacgaac
     1621 ccaactgcgc gcagcggcga atccgctcgt cccttcgggg cagattcggt ttgaagcgcg
     1681 gggcgcacgg tcggctccgt gggcggccgc tgcttggcct cgtggtcttt cgcggagacg
     1741 tcgaagtcga agcgatgagt gactctgctg ccaggtccga gcgtcacggc ccaggtgagc
     1801 gcgcggcccg acttgtcgcg tgcgacgaag gtgtgcgttc ccgcgcgcac gagcaacgtc
     1861 gtcgtcccga actcgagcgc ataatgctgc gcgccggcgg tcgacatcgc acgcgagtcg
     1921 atcacctcgc cttccgagct ggcaacgagc tcgacgttgg ccgcgttgcc acgagcgagc
     1981 agcatgtcgc gctcgatcgc cgcgcgctcg ctcttggcga cgttgcggcc cccgaccttc
     2041 aaatactctt cgaagtcggc gagggcttcc ccgtcgcgtt cgagcttcaa cgcgcagagc
     2101 ccgaggttgc ccaacacttt ccagctatgg ctcttttcgt atgcgagctt gaattggtcg
     2161 taggcgtcct gataattcgg cgcttcgctt tgcagtagct cgacgccgtt tcggaaatgt
     2221 ccctgagctt ctgcgctgac tttgggtgag gcacccgccg tatgccccgc cgagagccac
     2281 gcaaatagga acgcggtgct cgcgacgctc cgagttggct ccgcgataag ttctcgaaat
     2341 cctaccgtaa tccaaggatt tcgcgccggc ttcaggctcg actcgtcgag tccggcgcgc
     2401 gccgatcgaa tcgattccat ccacgtatcc tcctcccgtg cgaccaccgt gcgctcggat
     2461 cgttactcgc gcgttggtgt cttggcaacg aa
//
LOCUS       DASTKS010000095         5344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_906531, whole genome shotgun sequence.
ACCESSION   DASTKS010000095 DASTKS010000000
VERSION     DASTKS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906531"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /gene="xdhB"
                     /locus_tag="VFQ35_02025"
     CDS             complement(<1..2012)
                     /gene="xdhB"
                     /locus_tag="VFQ35_02025"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HET9929429.1"
                     /translation="MSALGKAVNHESARLHVTGTAAYVEELAMRQAGLCFAWPVLSPH
                     AHARVLHIDVDAALALPGVLTCLLASDVPGENDVGPVRHDEPLFPSEVCFHGQPVLWV
                     VGESFERARLGAERVQVEYEPLPALLGIEAAIHAESFHMPPERLRRGEPERALAAAPE
                     SLSGEIFIGGQEHFYLETQAALALPDDGASVLVHSSTQHPTETQEVVARVLGLDKHRV
                     TVQCTRMGGAFGGKETQANAWAAVAALASHKLNRPALVRLTRYQDMVATGKRHPFLAR
                     FKVGFEASGRITALFVELFSDGGFSLDLSSPVLFRAMFHVDNCYLLPNVQIDGRICKT
                     NTASNTAFRGFGGPQGMLVIEEILARIARHLELAPDVVRERNFYREGDTAHYGQPIQD
                     ADRVQRIWNELKASSDFAERAQAIEADNAKRRHLKRGIAITPVKFGISFTTAFFNQAG
                     ALVLIYKDGSVQVNHGGTEMGQGLHTKIAQVAAHSLGLPLGSIRVMATRTDKVPNTSA
                     TAASSGSDLNGAAVQNACEILKERLTEVAAQRFGASRESIVFEAARVFPRSREAEAVP
                     FRDIVSQAYLERVPLFASGYYRTPNIHFDRDSGRGKPFHYFAYGAAVSEVEVDGFTGQ
                     FRLLRVDILHDVGTSLSPLVDRGQVEGGFAQGVGWLTSEQLSWTNEG"
     gene            complement(2009..3415)
                     /locus_tag="VFQ35_02030"
     CDS             complement(2009..3415)
                     /locus_tag="VFQ35_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9929430.1"
                     /translation="MAIRFVLNGSPIEVNEAPLHSTLLQWLRANGYAGTKEGCAEGEC
                     GACAVALIASDPGAAGSRFEPVNSCLLPLCAVDGREVVTVEGVARSAEQLHPVQEAMV
                     AQGGSQCGYCTPGFVVSLFCEYYRPGRTEFDPESISGNLCRCTGYRPILDVGRNLPSP
                     EPHDERLVQLQRSTPAMSSVRNGAGGFERPTSLTELWQAFSARPDAVVLSGGTDLLVY
                     ANQRHQRWRALVSIEAIAELQELSSTDEEIVLGAAVPLTRVERWISRNAPELRLLEEL
                     FPLFSSRLIRNRATLGGNLATASPIGDSASALLALGASVELVSRAGARRVPLAEFFTG
                     YRRTVLQAQELIRAICIPRPVPEVQRFYKVSKRVLDDISTVAAAFALSLEADGLVRRF
                     GAAFGGIAATPLAAPSLERLALGKPWDEATLDVLLEEAPHIGTPQDDFRGSADYRRAM
                     VGKLLEKFFWETSGEASE"
     gene            3510..4337
                     /locus_tag="VFQ35_02035"
     CDS             3510..4337
                     /locus_tag="VFQ35_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET9929431.1"
                     /translation="MRDIVQALADLFENRRRGALATVVRTSGSVPQQPGARLLLREDG
                     TTLGTVGGGAIEREVLTALQRALETGGSELLVRDLGRDLGMCCGGRMEVFVEAIEPVP
                     RLWLLGAGHVARPTAALARSVGFEVVVVDEREELNTSERFPGCELCLDDPSDRLRRER
                     LGTSDWVVIVTHDHQLDERALEAAAANQSGYIGLVGSRRKVFRLVERVLARREGDIPY
                     ERIFAPIGLDIGAVTPEEIAVSIVAELVALRRGHHATHLRALTDEHLQRILAEEPIP"
     gene            4334..>5344
                     /locus_tag="VFQ35_02040"
     CDS             4334..>5344
                     /locus_tag="VFQ35_02040"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9929432.1"
                     /translation="MNAPNPSGLSELSQQIERDLMLLDYPRRPWLTPRVTRQGSPVYD
                     VIIVGAGQSGLATAFGLSRERVGNVLVIDQNPLDRAGPWLTFARMNTLRTPKYLTGPD
                     LGIPSLTPRAWYEAQFGIGSWEALGLLPKEMWASYLAWYRSTLKIPVRPETRVLELAY
                     DDDEKCFVVPCDGAKRERLFARRVVLATGIDGSGEWRVPESVSRALPRELYAHTHDPI
                     DFDALQGRRIAVLGAGASAFDNAATALEHGAREVHLLFRRRELVNVNAYRWAEFVGFL
                     KHHADLPDPEKWRFISQILRMGQLPPADTLARAKALPGFHLHPGSSWKRIAKQGSSVV
                     VET"
BASE COUNT          956 a         1769 c         1710 g          909 t
ORIGIN      
        1 ccctcgttcg tccacgaaag ctgctcactc gtgagccaac caacgccctg agcaaagccg
       61 ccctctacct gtccccgatc gacgagcggc gacagagacg tccccacgtc gtgcaggatg
      121 tcgactcgaa ggaggcggaa ttgccccgtg aacccatcga cttcgacctc gctcacggcc
      181 gcgccgtagg cgaagtagtg gaagggtttg ccgcgcccgc tgtcgcggtc gaagtggatg
      241 ttcggggtcc gatagtaacc actcgcaaag agcggcacac gttcgagata cgcctggctc
      301 acgatgtctc gaaacggcac cgcctccgct tcgcgcgagc gcgggaagac gcgggcggcc
      361 tcgaacacga tgctctcgcg actcgccccg aaacgctgag ccgccacttc ggtcaagcgc
      421 tccttcaaga tctcacaggc attttgcacc gcggcgccgt tcaagtcgga gccgctcgac
      481 gccgcggtgg cagaggtatt cgggactttg tcggtgcgag tcgccatcac ccggatggag
      541 cccaatggaa gcccgagcga atgcgcggcg acttgcgcga tcttggtgtg gaggccctgg
      601 cccatctccg ttccgccgtg gttcacctgc acactgccgt ccttgtagat gaggacgagc
      661 gcaccggcct ggttgaagaa cgccgtggtg aacgaaatac cgaactttac cggagtgatc
      721 gcgatgccgc gtttgagatg ccggcgtttc gcgttgtcgg cttcgatggc ttgcgcgcgc
      781 tcggcgaagt cgctcgaggc tttgagctcg ttccaaatgc gctgaacccg gtcggcgtct
      841 tggattggtt gcccgtagtg cgcggtgtct ccctcgcggt agaagttccg ctcgcggacg
      901 acgtcgggcg cgagttcgag gtgccgcgcg atgcgcgcca agatctcttc gatgacgagc
      961 atgccttgcg ggccaccgaa gccgcggaac gccgtgttgg acgcggtgtt cgttttgcag
     1021 atgcggccat cgatttggac gttgggcaat aggtagcagt tgtccacgtg gaacatcgcc
     1081 cggaacaaca ctggcgagct caaatcgagg ctgaagcctc cgtcgctgaa caactccacg
     1141 aagagggccg tgatgcgccc gctcgcctcg aagcccacct tgaagcgcgc gagaaatggg
     1201 tggcgcttac ccgtcgcgac catgtcttga tatcgcgtga gacggaccag ggccgggcgg
     1261 ttcagtttgt gcgaagccag cgcagcgacg gcggcccatg cgttggcttg cgtctctttt
     1321 ccgccgaacg cgccccccat gcgcgtgcac tgcacggtca cgcgatgctt gtcgaggccg
     1381 agcacgcgcg cgacgacttc ttgcgtctcg gtcggatgct gggtcgacga gtgcacgagc
     1441 accgaagcgc catcgtccgg cagcgcgagc gcggcctgag tttcgagata gaagtgctcc
     1501 tgtccgccga tgaagatttc gcccgagaga gactccggcg cggccgcgag ggctcgctcg
     1561 ggttcgccgc gtcgaaggcg ctcgggcggc atatgaaagc tctccgcgtg gatcgccgcc
     1621 tcgatgccga gcaatgcggg cagcggctcg tactcgacct gcactctttc agccccgagc
     1681 cgcgctcgtt cgaagctctc ccccaccacc cacagtaccg gctggccgtg aaagcaaacc
     1741 tcgctcggga acagcggctc gtcgtgacga accgggccga catcgttttc gccgggcacg
     1801 tcgctcgcca agaggcaggt gagcacgccg ggcagagcga gcgcagcgtc cacgtcgatg
     1861 tggagcaccc gcgcgtgagc gtgaggactc aacacgggcc acgcgaagca gagccccgcc
     1921 tgcctcatcg cgagctcctc gacataagcg gcagtgccag tgacgtgcag ccgcgccgac
     1981 tcgtgattta cggccttccc gagcgcgctc attcagacgc ctccccgctg gtctcccaaa
     2041 agaacttctc gagtaacttg ccgaccatgg cgcgcctgta atcggcgcta ccgcgaaaat
     2101 cgtcttgcgg ggtgccgatg tgtggagctt cctccagcag tacgtcgagg gtcgcttcgt
     2161 cccagggctt tccgagcgct aggcgctcga gcgaaggcgc ggccagtgga gttgcggcga
     2221 tgccaccgaa cgcggcccca aagcgccgta ccaaaccgtc ggcctcgagc gacaaagcga
     2281 acgctgcggc caccgtggaa atgtcgtcga ggacgcgctt gctaaccttg tagaagcgct
     2341 gcacctccgg caccggacgt ggaatgcaga tcgcgcggat gagctcttgc gcctgcaaga
     2401 ccgtgcgccg gtagccagtg aaaaactccg cgagcggcac tcgtcgagcc ccggcacggc
     2461 tcacgagttc gacgctggcg cccaacgcga gcaacgccga cgccgaatcg ccgattggag
     2521 acgccgtcgc caaattccct ccgagcgtcg cgcgatttcg tatcaagcgc gagctgaaga
     2581 gcggaaacag ctcttcgagc agccggagct ccggagcgtt acgcgagatc cagcgctcga
     2641 cgcgggtgag cggcaccgcg gcccccagca cgatctcttc gtccgtcgac gagagctctt
     2701 gaagctcggc gatcgcctcg atcgagacca gcgctctcca tcgctgatgc cgctggttcg
     2761 cgtataccaa caaatcggtc ccaccgctga gcacgaccgc gtcgggacga gccgaaaaag
     2821 cttgccacag ctcggtgagc gaagtcggtc gctcgaatcc gcccgcgcca ttgcggacgg
     2881 aggacatcgc aggagtgcta cgctgcagct gaacgaggcg ctcgtcgtgc ggctccggag
     2941 atggcaggtt gcgtccgacg tcgagaatcg gccggtaccc ggtgcaacga cagaggttgc
     3001 cactgatgct ctcgggatcg aactcggtgc gccctggccg gtagtattcg cagaacaagc
     3061 tgacgacgaa acctggagtg cagtacccac attgggagcc gccctgcgca accatggcct
     3121 cttgcaccgg gtgcaattgc tcggcgctgc gcgccacccc ttcgaccgtt acgacctcac
     3181 gaccgtcgac cgcgcacagg ggaagcaagc agctgttcac cggttcgaaa cggctgcccg
     3241 cggcgccggg atcgctcgcg atgagcgcga ctgcgcacgc accacattcg ccctcggcac
     3301 agccctcctt cgtccctgcg tagccgttcg ctcggagcca ctgcaaaagg gtcgagtgaa
     3361 gaggcgcctc gttcacctct atggggcttc cgttcagcac gaagcggatc gccatcgtga
     3421 ccatgctagc atgccatgtc gttggtttcg tccgaagctc ttggacgaaa cctcggcgac
     3481 ttcggtcgtc ttggaagctt gggaaccgag tgcgagacat cgttcaagcc ctcgctgatt
     3541 tgttcgaaaa ccgaaggcgc ggtgcgctcg cgaccgtggt gcgcacatcg ggttcggtgc
     3601 cacagcaacc gggcgcccgg ctgctgctgc gggaagacgg gacgacgctc ggcacggtgg
     3661 gcggcggagc catcgagcgt gaagtgctca cggctttgca acgtgccctc gaaacaggtg
     3721 gcagcgagct cctggtgcgc gacctcggtc gcgacctcgg catgtgctgc ggcgggcgca
     3781 tggaagtttt cgtcgaagcg atcgagccgg tgccgcgcct ctggctcctc ggcgccggcc
     3841 acgtcgctcg gccaaccgcg gccctcgcgc gcagcgttgg gttcgaagtg gtcgtcgtcg
     3901 acgaacgaga agagctgaac acctccgagc gctttcccgg ctgtgaactg tgcctggatg
     3961 atccgagcga ccgtctgcgc cgcgagcgac tcggcacgag cgattgggtc gtcatcgtca
     4021 ctcacgacca tcagctcgac gagcgcgcgc tcgaagccgc cgccgccaac caaagcggct
     4081 acattggcct cgttggcagt cgccgcaaag tctttcgctt ggtcgagcga gtcttggcga
     4141 gacgcgaggg cgacatcccc tacgagcgca ttttcgctcc catcgggctc gatatcggcg
     4201 ccgtcacgcc ggaggaaatc gcggtgagca tcgtggcgga gctcgtcgcg ctccgcagag
     4261 gacaccacgc cacccacctt cgagctttga ccgacgagca cctgcaacgg atcctcgcgg
     4321 aagagccaat cccatgaacg caccgaaccc gagtggactc agcgagctct cgcagcaaat
     4381 cgagcgcgat ttgatgctgc tcgactaccc gcggcggccc tggctgactc cgcgcgtcac
     4441 tcgtcagggc agcccggttt acgacgtcat catcgtcgga gcggggcaaa gcggcctcgc
     4501 aacggcgttt gggctgtcgc gcgaacgcgt cggcaatgtt ctcgtcatcg accaaaaccc
     4561 gctcgatcgc gcggggccgt ggctcacctt cgcgcgcatg aacacactgc gcacgcccaa
     4621 gtacctgacc gggccggatc tcggcatccc gagcctcacc ccgcgggcct ggtacgaagc
     4681 acagttcgga atcggcagct gggaggcgct gggactgctc ccgaaagaga tgtgggcgag
     4741 ctacctcgct tggtaccgga gcaccctgaa gatcccggta cgcccggaga cgcgcgtgct
     4801 cgagctcgcg tacgacgacg acgagaagtg cttcgtggtt ccttgcgacg gggcaaagcg
     4861 cgaacggctc ttcgcgcgcc gggtcgtcct cgcgactggg atcgacggct cgggcgaatg
     4921 gcgagttccc gagtcagtaa gccgcgcttt gcctcgcgag ctctacgctc acacccatga
     4981 cccaattgac ttcgacgcgc tgcagggccg acgcatcgcg gtgctcggcg caggcgcctc
     5041 cgcgttcgac aacgcggcca ccgcactcga gcacggcgca cgcgaggtgc acctgctttt
     5101 ccgaagacga gagctcgtca acgtcaacgc ctatcgctgg gcagagttcg ttggcttttt
     5161 gaagcaccac gcagacctgc ccgaccccga gaagtggcgc ttcatctcgc aaatactgcg
     5221 gatgggtcag ctccccccgg cggatacgct cgctcgggcg aaggcgctgc ccgggttcca
     5281 cttgcatcca ggctcgtcgt ggaagcgaat cgcgaagcaa ggttcatccg tcgtcgtaga
     5341 gacc
//
LOCUS       DASTKS010000096         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_683742, whole genome shotgun sequence.
ACCESSION   DASTKS010000096 DASTKS010000000
VERSION     DASTKS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683742"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /gene="galE"
                     /locus_tag="VFQ35_02045"
     CDS             complement(<1..655)
                     /gene="galE"
                     /locus_tag="VFQ35_02045"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET9929433.1"
                     /translation="MNILVTGAAGYIGSHAVRALLAASHRVVALDNLSRGHRVAVPAD
                     ATFAELDVRDTAKVTQLLGDERIECVMHFAAFANVGESVENPLLYYDNNTGGTLSLLR
                     ALEQSKCRRFVFSSTCATYGEPDSMPIREETAQNPINPYGASKLFSERMVRELSRKLP
                     SLSCAMLRYFNVAGAAADGTLGEHHDPETHLIPVILQAALGARDKVIVFGDDYPTPDG
                     "
     gene            complement(741..2192)
                     /locus_tag="VFQ35_02050"
     CDS             complement(741..2192)
                     /locus_tag="VFQ35_02050"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929434.1"
                     /translation="MTFELEQREREAFLDLLSRLVAPSGALAGFFSSEAGIGVARAPG
                     RLDVMGGIADYSGSLVLELPLAEGACAAAQLHPDPELWVASVAAEAPHDLREARLPIG
                     PLFHADYEQAARLLRRAGLPDFLPYLAGLVVPLRSKGLPAAGGVRLVVSSRVPEGRGV
                     ASSAAIEVAGFFALAAAAGLHVEASDAAELCQRVENLVVGAPCGIMDQMAVACGRREA
                     LLELLCQPASMLGFLRVPPELAFFGIDSGVRHSVSGREYRTVRSAAFMGYRLLVEHLG
                     FGATRNAEGALLIDDARFRGYLANVTPSELDSEFRDVLPERLSGAEFIERFGDITDSA
                     TRLEPTVSYPVRAATEHPIREHHRVRCFAELLGAASSASAAHLMGELMYQSHASYSAC
                     GLGSAATDRLVRLVREAGIDSGLYGAKITGGGAGGTVAVLARRDASRALAHIAERYAE
                     ESGQKPRIFSGSSAGAAEFGTMNLPGATLAPSV"
     gene            complement(2189..>2794)
                     /locus_tag="VFQ35_02055"
     CDS             complement(2189..>2794)
                     /locus_tag="VFQ35_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929435.1"
                     /translation="IQSELPLLKQRYRDQLVARRGHGVEAIRVDMVDGARQPLSLRPS
                     RARELSHAFATFCRFFPGVRDEIAALDDELMFALMGPPPEKKALCFEDQYTSTGGQFY
                     ELMAGHDRFIADLRPLFAPLLAERGLPAPLCCHPYDVATALIAEELGVCLRSPWGEPF
                     DVRLDIDSDVAWVGYANAELMRSVEPVLISVLERRGHRRSG"
BASE COUNT          536 a          925 c          906 g          427 t
ORIGIN      
        1 ttccgtccgg tgtcgggtag tcgtctccga acacgatgac tttgtcgcgg gcgccgaggg
       61 ccgcttgcaa aatcaccggg atgaggtgcg tttcggggtc gtgatgctcg ccgagcgtcc
      121 cgtccgcggc ggcgcccgcg acgttgaagt agcgcagcat tgcgcacgac aagctcggga
      181 gctttcgcga tagctcacgg accatgcgct cggaaaagag cttggaagcg ccgtacggat
      241 tgatcgggtt ctgcgccgtc tcctcgcgga tcggcatcga atccggttcg ccgtacgtgg
      301 cacacgtcga gctgaacacg aagcgtcgac acttgctctg ctcgagtgcg cggagcaggc
      361 tcaacgtccc gccggtgttg ttgtcgtagt ataagagagg gttctcgacc gactcgccca
      421 cgttggcgaa cgcggcgaaa tgcatcacgc actcgatgcg ctcatcgccc aggagctgcg
      481 tcaccttcgc cgtgtcgcga acgtccaact ccgcgaaagt cgcgtctgcg ggtacggcga
      541 cgcggtggcc acggctcaga ttgtcgaggg cgacgactcg atgagaagcc gcgaggagcg
      601 cccgcacggc atgggagccg atgtaaccgg cggcgccggt aaccaagata ttcatcccta
      661 agtgtcctga gtcagcgagt ccgccccaga cgaaggttca tcagctcgcg tgatgtaacg
      721 aatctcgagc gtaagcagac ctaaaccgaa ggagcaaggg tcgcgccggg taaattcatg
      781 gttccaaact cggccgcgcc ggcagaggac cccgaaaaga tccgaggttt ctggcctgat
      841 tcttccgcgt aacgctcggc aatgtgcgct agggcgcgcg acgcatcccg ccgagccagc
      901 acggccacgg tgcctcccgc cccaccaccg gtgatttttg ccccatagag tccgctgtcg
      961 atccccgctt cgcgcaccaa tcgaaccagt cggtcggtcg cggcagagcc gagcccgcac
     1021 gctgaataac tcgcgtggga ttgatacatg agctcaccca tcaggtgggc cgcactcgct
     1081 gacgaagcag cgccgagtag ctcggcgaag caccgaacgc ggtggtgttc acggattggg
     1141 tgttcggtcg ccgcccgcac cgggtagctc acggtcggct cgagccgcgt tgccgaatcg
     1201 gtgatgtccc cgaagcgttc gatgaattcg gcgccgctga gccgttctgg caagacatcg
     1261 cggaactcgg agtcgagctc cgagggagtg acgttcgcca gatatcctcg aaagcgagca
     1321 tcgtcgatga ggagcgcgcc ctcggcgttt cgcgtcgcgc cgaagcccaa gtgctcgacg
     1381 agcaagcgat agcccatgaa cgccgccgag cgaacggtcc tgtactctcg cccgctcacc
     1441 gagtgacgca cgcccgagtc gataccgaag aacgcaagct ctggcggcac gcgcaaaaag
     1501 ccgagcatcg acgcgggctg acaaagcagc tcgagcaacg cttcacgccg cccgcacgcg
     1561 accgccatct gatccatgat gccgcacggg gcccccacga cgaggttctc cactcgctgg
     1621 cagagctccg cggcgtcgct cgcctcgacg tgaagccccg ctgccgccgc aagtgcgaaa
     1681 aagcctgcga cttcgatcgc tgcggacgac gcgacgcccc gcccctcggg tacgcgcgac
     1741 gacacgacga gtcgcactcc gccggcggct ggcaagccct tcgaacgcag cggcacgacg
     1801 aggccggcca agtatgggag gaaatcgggg agacccgcgc gacgcagcaa gcgcgcagct
     1861 tgctcgtagt cggcgtggaa caacggccca atcggaaggc gcgcctctcg gaggtcatga
     1921 ggcgcttcgg ccgcgacgct cgcaacccac agctcggggt cggggtgcag ctgcgcagcg
     1981 gcgcaggctc cctcggcgag cggcagctcg agcaccaaag aacccgagta gtcggcgatt
     2041 ccgcccatca catcgagccg gccgggagct cgcgcaacgc cgatgcccgc ctcgctcgag
     2101 aagaagcccg cgagcgcgcc actcggagcg acgagccgac tcagcagatc gaggaacgcc
     2161 tcacgttcgc gctgttcgag ttcgaaagtc acccagacct cctgtggccg cggcgctcga
     2221 gcaccgagat caataccggt tccacgcttc gcatgagctc cgcgttcgcg tatcccaccc
     2281 aagcgacgtc ggagtcgatg tcgagtcgta cgtcgaacgg ctcaccccag ggtgaacgca
     2341 gacacacccc gagctcttcg gcgatgagcg cggtggcaac gtcgtacggg tgacaacaga
     2401 gcggcgccgg cagcccgcgc tccgcgagca acggcgcgaa caatggccgt aaatcggcga
     2461 tgaagcgatc gtgcccggcc atgagctcgt aaaattgtcc gccggtcgag gtgtactgat
     2521 cctcgaagca gagcgccttc ttttccggcg gcgggcccat caacgcgaac atcagctcgt
     2581 cgtcgagggc ggcaatctcg tcgcgaacgc cggggaaaaa tcggcaaaat gtggcgaagg
     2641 cgtgcgagag ctcgcgcgcg cgcgagggcc gcagcgaaag cggctggcgg gcgccgtcga
     2701 ccatgtccac gcgaatcgct tcgacgccgt gccctcgccg cgcgacgagc tggtcgcggt
     2761 agcgttgctt caagagcggc agctcgctct gaat
//
LOCUS       DASTKS010000097         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1576716, whole genome shotgun sequence.
ACCESSION   DASTKS010000097 DASTKS010000000
VERSION     DASTKS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576716"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VFQ35_02060"
     CDS             <1..337
                     /locus_tag="VFQ35_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929436.1"
                     /translation="LKRVTPELAPELYELASADVRGKGKDATQDLAQIEALKAHAERL
                     IAEGAALSVRDLKVDGRTIMQAFDRRPGPWLGELLRTLLDEVVENPSLNERERLLERA
                     RSLVAANPN"
     gene            549..1883
                     /locus_tag="VFQ35_02065"
     CDS             549..1883
                     /locus_tag="VFQ35_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929437.1"
                     /translation="MKLRLAFGVVLAAACGGRAVDGDVPLGCNTDLECKGDRICVAGR
                     CEDAALGASGGRTGAGGSAGFPMFGGASFGGSPFGMGGASFGSGGSRVSAQGGATSGT
                     GGASIGATPTRLDIDASALIADPLRARLYATVRGNAASHGNEVVVIDARLDTVLTSLF
                     VGSDPDSLALSDDGTRLWVGLHGALSIREVDLTQFPPVAGAQYVVPPGDFAFDAVHAG
                     PMVVLPGEPESVAISLHYDGLSPSFAGLAVIDSGNPREKQTPSHTGASRLTGGPPGFL
                     FGFNDQHTGFEFFSIAVDASGVTQSETRGLVDGFSSDIRYDGGYVVATSGQVVDVTKP
                     SAPVRAGKFPFTGQVVPHGANSRVLMLSSVSAGTSSSSGSGNNMLVLRNLNLSTFRED
                     GETPINGRFNSVQDFVEAAPGVFAFIDANTASFGSTPSPSNVYVFSVPEFAE"
     gene            1897..3297
                     /locus_tag="VFQ35_02070"
     CDS             1897..3297
                     /locus_tag="VFQ35_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929438.1"
                     /translation="MAIHRKARRVALRVDVLTRARRRLPTFALPLTLVVSCGARNIHG
                     SDSDGCETDFDCKGERVCIAGACQNLSRVGGGGQANLGGFGAGASSGGTALSSGGAFS
                     SGGAPLNMGGFTTAFGGSTGVGSSGSGSIVLQAPKEVAVEAKALVADPLRGKLYAVVS
                     SSASRYANELVVIDAARGVVDTSVAVGSDPDTLAISDDGSRLWVGLSGALSIREVDLT
                     VSPPLPGSQFALPPSRSFGNATRAGKIVVLPGTPQSVVATLLFASGNSIAGVAAFDDG
                     VPRSTSLNDYPMPYQLTGGPPGFLFGFNDHDTGFEFVTLAIDAMGVRATRRSGLIEGF
                     ETTIAYDAGLVLASSGHVMTVADPTAPARAGKFAFVGLVIPHVAESMAVMLSSVAVSQ
                     TFDSDNRLVLRRLNIGTFREDFEASLPGKYGDVRDFVEPIRGTFAFLAPAIGDFDFGT
                     PRTTYVNIFKAPKFAQ"
BASE COUNT          580 a         1224 c         1165 g          615 t
ORIGIN      
        1 gctcaagcgc gtcactcccg agctcgcgcc cgaactctac gagctcgctt ccgccgacgt
       61 acgcggcaaa ggcaaagacg cgacccaaga cctcgcgcag atcgaagcgc tcaaagctca
      121 cgccgagcgc ctcatcgccg aaggcgccgc cctcagcgtg cgcgacctga aggtcgacgg
      181 tcgcaccatc atgcaagcat tcgatcgccg ccccgggccg tggctcggtg agctcttgcg
      241 cacgttgctc gacgaagtcg tcgagaaccc gtcgctcaac gagcgcgagc ggttgctcga
      301 gcgcgcgcgc tcgctggtcg cggcgaaccc gaactgagcc atggtttggg gcaagcactg
      361 aaacgagcgc gaaaacgcgc gcggtgcggc gatcgccacc cgagcgcggc tcctcacggg
      421 tccggagccg cgcccgcccc gccccgtagt tcacggccca cccatcctcc tcgcgaaggc
      481 cgacgcacgt ccgccgcgaa gccgacgcac cttgagaaaa gggtcgattt catgctgaac
      541 ttccaggcgt gaaactgcgc ttggcgttcg gggtggtgct cgccgcggcc tgcggcgggc
      601 gcgcggtaga cggcgatgtg ccgctcgggt gcaacacgga tctcgaatgc aagggtgacc
      661 gcatttgcgt cgcggggcgc tgcgaagatg ccgcgctcgg cgcgagcggc ggacgcaccg
      721 gcgctggcgg ctccgccggg ttcccgatgt tcgggggcgc gagcttcggc ggatcgccct
      781 tcggaatggg cggtgcgtcg ttcggcagtg gcggctcgcg cgtcagcgcg caaggcggag
      841 ctactagcgg cacgggcggc gccagcatcg gcgcgacgcc aacccggctc gacatcgacg
      901 cgagcgcgct catcgccgat ccgctgcgcg cgcgcctgta tgcgacggtg cgggggaacg
      961 cggcgtcgca cggcaacgaa gtcgtcgtca tcgacgcacg gcttgacacg gtgctgacgt
     1021 cgctgttcgt gggctccgat ccagactctc tcgccctttc ggacgatggc acccgacttt
     1081 gggtgggcct tcacggcgcg ctatccattc gagaggttga tctaacgcag tttccaccgg
     1141 tcgcaggcgc tcagtacgtc gtccctccag gcgattttgc attcgacgca gtccacgcag
     1201 gcccgatggt cgtcttaccg ggcgagcccg aaagcgtcgc catctcgctt cactacgacg
     1261 gcctcagccc gagcttcgcc ggcctcgccg tgatcgactc cggcaacccg cgcgagaagc
     1321 agactccctc gcacacgggc gcctcgcgcc tgactggcgg gcccccgggg tttctctttg
     1381 gattcaacga ccaacacacc ggcttcgagt tcttcagcat cgcggtggac gcgagcggcg
     1441 taactcaaag cgagacccgt ggcttggtgg acggcttcag ctccgacatt cgttacgacg
     1501 gcggctacgt cgtggccacc agcggacagg tcgtcgacgt cacgaagccg agcgcgcccg
     1561 tgcgcgcagg taagttcccg ttcacgggcc aggtcgtgcc gcacggcgcg aactcgcgcg
     1621 tcctgatgct ctcctccgta agcgctggaa ccagctcttc gagcggcagc ggcaacaaca
     1681 tgctcgtctt gcgcaacttg aacttgagca ctttccgaga agacggcgaa acccccatca
     1741 acgggcgttt caactccgtg caagacttcg tcgaggccgc gcccggggtc ttcgcgttca
     1801 tcgacgcgaa caccgcgagc ttcggcagca ccccgagccc gagcaacgtt tacgtcttct
     1861 cggtgcccga attcgccgag taggttggtg ctcgacgtgg caattcaccg aaaagctcgc
     1921 cgcgtcgcgt tgcgggtcga cgtactgacc cgggctcgtc gacgactgcc cacgttcgcg
     1981 ctgccgctca cgctcgtagt gagctgcggt gcacgcaaca tccacggctc ggattcggat
     2041 ggctgcgaga ccgatttcga ctgcaaagga gagcgcgttt gcatagccgg ggcgtgccaa
     2101 aatctttctc gcgttggcgg cggcggccaa gcgaacctgg gcggcttcgg tgcgggagcg
     2161 agctccggtg gcaccgcgct gtcgtccggc ggcgcgtttt cttcgggcgg cgcacctttg
     2221 aacatgggcg gcttcacgac cgcgttcgga ggttcgaccg gggtcgggtc gagcgggagc
     2281 ggctcgatcg ttctgcaagc gccgaaagag gtcgccgtcg aggccaaggc gctcgtggcc
     2341 gatccgttgc gcgggaagct gtacgcagtc gtctcgagca gcgcgagtcg ctatgcgaac
     2401 gagctcgtcg tcatcgacgc ggcccggggc gtggtcgata cgagcgtagc cgtcggctcg
     2461 gatccggata cgctcgccat ctccgacgat ggttcacgac tctgggtcgg gctgagcggc
     2521 gcgctgtcga tccgcgaagt cgacttgacg gtttcgccgc cgctgccggg ctcacagttc
     2581 gcgcttcccc cgagccgatc gttcggcaat gcgacccgcg ccggtaagat cgtggtgttg
     2641 ccgggaacac cgcaaagcgt ggtcgcgacc ctgctctttg cttcgggcaa tagtattgcg
     2701 ggagtcgcag cgttcgacga cggggttccg aggtcgactt cgctgaacga ctaccccatg
     2761 ccttaccaac tcacgggcgg tcccccgggc tttcttttcg gcttcaacga ccacgacacc
     2821 ggcttcgagt tcgtcaccct cgccatcgac gcgatgggag ttcgcgcgac tcggcgctcc
     2881 gggttgatcg agggcttcga aacgacgatc gcttacgacg cggggctcgt cctggcctcg
     2941 agcgggcacg tcatgaccgt cgccgatccc accgcgcctg cgcgcgcggg aaagttcgcg
     3001 ttcgtcgggc tcgtcattcc ccacgtcgcc gaatccatgg ccgtgatgct cagtagcgtc
     3061 gccgtgagcc aaaccttcga ctccgacaac cggctcgtgc tgcgccgact gaacatcggc
     3121 acttttcgcg aagacttcga agcgagcctc cccggcaagt acggcgacgt ccgagatttc
     3181 gtcgagccga tacgtgggac attcgccttt ttggcacccg ccatcggaga cttcgacttc
     3241 ggcactccga gaacgaccta cgtgaacatt ttcaaagcac cgaagttcgc acaatgaaag
     3301 cgccgcacca ggactcgagc ctccctcgac gttttcctcc cccggcgtga gcgcgttcgc
     3361 tgcaagcggt gcgatgcgca gcgtctcccg cgccgcaccg ctcgacgttt cctcccgcag
     3421 cgtgagcgcg tccgctgcaa gcggtgcggt gcgcagcgtc ttccgcgccc cgccgcttgc
     3481 agcgccttca ccaccagcct gacttagggc ccgcgaatga acaagtcagt cgcgagcggg
     3541 ccgagaccgc agcgaggcgg gcgcgtgagg agagagcacc gcag
//
LOCUS       DASTKS010000098         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_237340, whole genome shotgun sequence.
ACCESSION   DASTKS010000098 DASTKS010000000
VERSION     DASTKS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237340"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1754)
                     /locus_tag="VFQ35_02075"
     CDS             complement(99..1754)
                     /locus_tag="VFQ35_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929439.1"
                     /translation="MATAAIVGKEFEVAVLVGASAGSEAAVRLALSEALTASFVLPTR
                     ERGQAAFVFAHDEIAEVLVDSVPREQLQQLHRRVARSLERLHADRPAEIALHYDAAGE
                     AAEAYRWTQRAAIAAERVYAYATARSLLHVAARNATTAAELAEVRVSLAHVCETGGRF
                     DEVEELCDLAIEWFEGQGDERRALTLRRMRERARMELGQPARVTLEALTVLDAEAQRL
                     GFDRERVSILLLLSQIQGRLGDKRTAERMAADGVEMAETLGDPALLADAVTRLGNCVS
                     SEAPGRAYPIFRRALSLYESIGDIRGQARMYGNIGVAAQWESRLDEARNAYGRGIAMA
                     RAGGMPDIWGVMAVNLGVLLQKCGEYDRAKELFSEALELFAAVKHSEFQLAALFNLAH
                     AERELGAWEAATELYETTIPLAQRIGQADIEIGAIAGAGLCALELDKLDAARVAASDL
                     VSRMRNRTDWFQGRELVEALFIRIDAVEERHVQALTRFATAVSLAEAADVYSAAWLVA
                     VCSESLTEADPLTVHRFVDEYDGRVKKLGYAEMTRRFDVLAHR"
     gene            1866..>2119
                     /locus_tag="VFQ35_02080"
     CDS             1866..>2119
                     /locus_tag="VFQ35_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929440.1"
                     /translation="MPDRTGLDERTEELCDRQRVAFRLPIDIREELATHLLPVERRLE
                     PLLHFVARETRDRHLAHQLGACREATFCVAVVHGYGVPRDA"
BASE COUNT          366 a          773 c          639 g          341 t
ORIGIN      
        1 aatacgattc gagccggacg ggtgaccggc gtcgagaccg cgaagatagg ccgtattgag
       61 agctatcgcc aggaataacc agaaggccgt gacaggagtc accgatgcgc gaggacgtcg
      121 aaccggcgag tcatctcggc atagccaagt ttcttgacac gcccgtcgta ttcgtccaca
      181 aaccggtgca ccgttagcgg gtccgcctcg gtgagactct cagaacatac ggccacgagc
      241 caagcggcgc tgtaaacgtc cgcggcttcc gcgagggaca ccgcggtcgc aaagcgcgtg
      301 agcgcctgca cgtgccgctc ctcgaccgca tcgatacgga tgaacaacgc ctcgacaagc
      361 tctcgtccct gaaaccagtc cgtccggttg cgcatgcgcg agacgagatc ggacgcagcg
      421 acacgagcgg catccagctt gtccaactcc agagcgcaca gacctgcgcc ggcaatcgcg
      481 ccgatctcga tgtcagcttg gccgatcctc tgggcgaggg ggattgtcgt ttcgtagagt
      541 tcggtcgctg cttcccaagc gccgagctca cgctccgcgt gcgccaaatt gaataatgct
      601 gcgagctgga attcgctgtg cttcactgcg gcgaagagct cgagcgcttc actgaacagc
      661 tctttcgcac ggtcatactc gccgcacttc tgaagcagca cgccgaggtt aacggccatg
      721 acaccccaga tgtcgggcat gccgcccgct cgggccatcg cgatgccgcg gccataggcg
      781 ttgcgcgcct catcgagcct tgactcccac tgtgccgcaa cgccgatatt gccgtacatc
      841 cgcgcctggc cacgaatgtc accgatgctc tcgtacaacg agagcgcacg ccgaaagatc
      901 ggatatgcac gtccgggtgc ctcgctcgaa acgcagttcc ccaaccgcgt caccgcatcc
      961 gccaacagcg ccggatcacc aagcgtttcc gccatttcca ccccgtccgc cgccatccgc
     1021 tcggccgtcc gcttgtcccc cagccggccc tggatctgcg acagcaggag cagtatcgac
     1081 acacgctctc gatcgaaccc gagccgctgc gcctcggcgt ccagcaccgt cagcgcctcg
     1141 agcgtcacgc gcgccggctg cccgagctcc atccgcgccc gctcccgcat tcggcggagc
     1201 gtcaacgccc gccgctcgtc gccctggccc tcgaaccact cgatagccag atcgcatagc
     1261 tcttcaacct cgtcgaaccg cccgcccgtc tcgcagacat gcgcgagcga cacgcgcacc
     1321 tcggcgagct ccgccgcggt cgtcgcgttg cgcgccgcga cgtgcagcaa cgagcgcgcg
     1381 gtggcgtacg cgtacacgcg ctccgcggca atcgccgctc gctgcgtcca gcgatacgcc
     1441 tccgccgcct cgcccgccgc atcgtagtgc aaggcgatct cggccggtcg atccgcgtgc
     1501 agccgctcga gcgagcgcgc gacccggcgg tgaagctgct ggagttgctc gcgcgggacg
     1561 ctgtcgacca gcacctcggc gatctcgtcg tgggcgaaca cgaacgccgc ctggcctcgc
     1621 tcgcgcgtcg gaaggacgaa gctcgcggtc agcgcctcgc tcagcgccag tcgcaccgcg
     1681 gcttcgctgc ccgccgacgc gcccacgagc accgcgacct cgaattcctt tccgacgatc
     1741 gcggccgtgg ccaagacggc ttgcgtgctc gacgagaatc ggctcaagcg atgcgcgatc
     1801 aacgcgtcgc gaccggttcg cagtcgcagc tcggagacgg gcgtccactc ccaacgcgtt
     1861 ccgttgtgcc agatcgcacc ggtctcgacg agcgcacgga ggagctgtgc gatcgtcagc
     1921 gggttgcctt ccgtctgccg atagacatac gcgaggagct cgcgacccac ctgttgccgg
     1981 tggaacgccg cctcgagcca ctgcttcact tcgtcgcgcg tgagactcga gatcgtcatc
     2041 tcgcgcacca actcggggcg tgccgcgagg caacgttttg cgtcgctgtc gtgcacggct
     2101 acggcgtgcc gcgcgacgc
//
LOCUS       DASTKS010000099         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_462186, whole genome shotgun sequence.
ACCESSION   DASTKS010000099 DASTKS010000000
VERSION     DASTKS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462186"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFQ35_02085"
     CDS             complement(<1..267)
                     /locus_tag="VFQ35_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929441.1"
                     /translation="MVQKNVFSPTRSWIAAVCGALALGALSPRALAEGATSGAAVVHD
                     LLSQPDALRNRLSQVSPEVGAARSRIAQAEADASTTRLIPNPIVD"
     gene            complement(467..1741)
                     /gene="eno"
                     /locus_tag="VFQ35_02090"
     CDS             complement(467..1741)
                     /gene="eno"
                     /locus_tag="VFQ35_02090"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9929442.1"
                     /translation="MPEILQVSAREILDSRGNPTVEAEVHTTSGWGRAAVPSGASTGA
                     HEANELRDGDKKRYLGKGVKQAVENVNQKLGPAVIGLSALDQAAIDHRLLELDGTPNK
                     AKLGANAILAVSMATARAAADTVGLPLWRYLGGVQARVLPTPLMNIINGGVHAVSGLE
                     IQEFMIVPLGLATFGEALRAGVEIFHNLKSILKKEGMATSVGDEGGFAPRLASNEEAI
                     KRVVQAIEAAGYTPGQDVAIGLDCAASEFFGEDGKYTFDKKKVTPAELAGIYGELCEK
                     YPIVSIEDGFAEDDWDGWKLITDKLGARVQLVGDDLFVTNKERLAKGIERKIANSILI
                     KVNQIGSLTETLDTIRLASTAAYTSIISHRSGETEDAFIADLAVATNAGQIKTGSLSR
                     SDRVAKYNQLLRIADELGDQQVYSGRTPFTRR"
     gene            2239..2991
                     /locus_tag="VFQ35_02095"
     CDS             2239..2991
                     /locus_tag="VFQ35_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9929443.1"
                     /translation="MSSEAEPKSAPPAAAASSGASVSGTSASNTGVVVNGVRYVRAPV
                     PVRFPDSELVPETKRHLEQRSLLYEILKLHFAESAIIGCDQFVYWDPTDPKQCLAPDA
                     FVRLGAPDCEFRSWKVWERGAPDVAVEIISHADARDKQWEPKFEKYRRLGVRELVRFE
                     GDHEGSISLRIWDSIDDDLVEREGVQSVAASHVLPGAWVAIIDSHGVPRLRLSRDADG
                     KDLYPTPTERAELAEARIRELEAELARRGERP"
     gene            complement(3305..>3507)
                     /locus_tag="VFQ35_02100"
     CDS             complement(3305..>3507)
                     /locus_tag="VFQ35_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929444.1"
                     /translation="LSFSTESADEPRDELSPSVLSAASGDHLALGFKSGFVRFRISDL
                     GIFWVQQFADPEVPRYAFYRKF"
BASE COUNT          583 a         1179 c         1099 g          646 t
ORIGIN      
        1 gtcgacgatc gggttcggga tgagccgcgt cgtcgatgcg tcggcctccg cctgagcaat
       61 ccgcgagcga gcagcgccga cttccgggct cacctgggag aggcggttgc ggagcgcgtc
      121 tggttggctg aggaggtcgt gaacgacggc agcgccactc gtcgcccctt cggctagagc
      181 gcgcggactc aacgcgccga gtgcgagcgc gccgcacacg gcggcgatcc aggagcgggt
      241 tgggctgaat acgttttttt gaaccactga gaagccgcgc tctcctcagc aggtttcgcg
      301 ccatgacctg cctcactcga aaacctcgag aaacaggcgg tgcctacatt tggctacccg
      361 ggcacgcgtt gaattaatat ttcagcgcgg cccagcgaaa gcacaaaaga ctttcgccgc
      421 cccagactgc tccgggacgg cgaaaggctg acggtgacgc ggattttcag cgacgggtga
      481 acggggtgcg gcccgagtac acctgctggt cgccgagctc gtcggcgatg cggagcagtt
      541 ggttgtactt cgcgacgcgg tcgctgcggc tcaagctgcc cgtcttgatc tgaccggcgt
      601 tcgttgcgac ggcgagatcg gcgatgaagg cgtcctcggt ctcgcccgag cgatgcgaga
      661 tgatcgacgt gtacgccgcc gtgctcgcga ggcgaatcgt gtcgagcgtt tccgtcaggg
      721 agccgatctg gttcaccttg atcaagatgc tgttcgcgat cttgcgctcg atgcccttcg
      781 cgaggcgctc cttgttcgtg acgaagaggt cgtcgccgac gagctgaacg cgcgcgccga
      841 gcttgtcggt gatgagcttc caaccatccc agtcgtcctc ggcgaagccg tcttcgatgc
      901 tgacgatcgg gtacttctca cagagctcgc cgtagatgcc cgcgagctcg gcgggggtca
      961 ccttcttctt gtcgaaggtg tacttgccgt cctcaccgaa gaactcgctc gcagcgcaat
     1021 cgaggccgat cgcgacgtct tggcccggcg tgtaaccggc ggcttcgatg gcctgaacga
     1081 cgcgcttgat ggcttcctcg ttcgaggcga gacgcggcgc gaacccgccc tcgtctccca
     1141 ccgacgtcgc catgccttcc ttcttcagga tcgacttcag gttgtggaaa atctccacgc
     1201 cggcacgcag cgcttcgccg aacgtcgcga ggccgagcgg cacgatcatg aactcttgga
     1261 tctcgaggcc gctgaccgcg tggacgccgc cgttgatgat gttcatcagg ggggtcggca
     1321 gcacgcgcgc ctgaacgccg ccgaggtagc gccagagcgg caagcccacc gtgtccgccg
     1381 ccgcgcgggc cgtcgccatc gacaccgcca agatcgcgtt cgcgccgagc ttcgccttgt
     1441 tgggcgtgcc gtcgagctcg agcaaacggt gatcgatcgc cgcttggtcc agcgccgaca
     1501 agccgatgac ggcggggccg agcttctgat tgacgttctc gacggcctgc ttcacgcctt
     1561 tgcctaggta gcgcttcttg tcgccatcgc ggagctcgtt cgcctcgtgc gcgccagtgg
     1621 aggcgccgct cgggacggca gcgcggcccc agccactcgt cgtgtgcact tcggcttcga
     1681 cggtcgggtt cccgcgggaa tcaagaattt ctcgggcact gacttggagg atctccggca
     1741 tgggcgccgc ttaacatgga tttcgcgggc atgcgagacc tggcgcagcc cgtttctgcc
     1801 aggtttcgcc tccttccgcg aaccagcgat tcacgccggc accgttactg ctcaaacgct
     1861 ctcgctttcc gcccgcgccc cgccgccgct ctcctttagt gccgacgaac gctcggaatg
     1921 atcatggttg gggggcctca ccgtaaaggg cgcgcaagac gtcactgatc gatgtcagcg
     1981 cagactccga gcgccccata cgtctctccc accgcgcccg ccccccaccg aaaagcccgc
     2041 accgaaaagt caccgcgtcg ccctcgccgc gccgcctcgc cgcgtcgccc aagcggtgat
     2101 gctcgctgcc cgctcgcgtc acccgagcgg tgatgctcgc tgcccgctcg cgtcacccgt
     2161 ctgggcgaaa ccgcgcaact cgcgctgcct cccgcccgac ctgcgctcat cggatgcgaa
     2221 aggtatattc gaatgacgat gagctcggag gcagaaccca aatcggctcc tccggccgct
     2281 gcggcttcat ccggcgcgtc ggtttcgggc acgtccgctt ccaacacggg cgtcgtcgtg
     2341 aacggcgtcc gctacgtgcg cgcaccggtg cccgttcgct ttccggactc cgagctcgtg
     2401 cctgaaacca agcggcacct cgagcagcgc tcgcttcttt acgagatcct gaagctccac
     2461 ttcgccgaga gcgcgatcat cgggtgcgac caattcgtct attgggatcc aacggatccg
     2521 aagcagtgcc tcgcccccga cgcgttcgtc cgcctgggcg cgccagattg cgagtttcgc
     2581 agctggaaag tctgggagcg cggagcgccc gacgtcgccg tcgaaatcat cagccacgcc
     2641 gacgctcgcg acaaacagtg ggaacccaag ttcgaaaagt accgccggct cggcgtgcgc
     2701 gagctcgtcc gcttcgaagg cgatcacgag ggcagcattt cgctacgcat ctgggactcc
     2761 atcgacgacg acttggtcga gcgcgaaggc gtccaaagcg tcgctgcgag tcacgtcctg
     2821 cccggcgctt gggtcgccat catcgattca cacggcgttc cacgcttgcg cttgagtcgc
     2881 gatgctgacg gaaaagatct gtacccaact cccaccgaac gcgccgagtt ggcggaagcg
     2941 cgcatccgtg agctcgaggc cgagctcgcg cggcgcggcg aacggccctg agccgtaggt
     3001 tggcgaagga gtgctcgggg cgaggaacac gccggtcccc gagcgtcatg atttgccggc
     3061 gttcgtacgt tttcgttcac gcctctcacc gattcgagtc gccgccccgc ccccgctgct
     3121 ttgccccgcg ctcctaccac cgaaaacgag caatcgactc ccttgtaatc taccggtggc
     3181 ggacgagcct tcgactcttc tgttcatcga cgactctgag tgagtgctcg cgtcgtccgg
     3241 tttcatcacg caatcgactg gcgccgtcga attgcggctc tttgccgtcc cgccgggggg
     3301 taagttagaa cttccggtaa aacgcgtagc ggggaacctc gggatccgcg aactgttgaa
     3361 cccagaagat accgaggtcc gatatacgaa acctcacgaa gcccgacttg aagccgagcg
     3421 cgaggtggtc gccgctcgcc gcgctcaata ccgacgggga gagctcgtcg cggggctcgt
     3481 ccgcagattc ggtcgaaaag ctgagca
//
LOCUS       DASTKS010000100         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_684986, whole genome shotgun sequence.
ACCESSION   DASTKS010000100 DASTKS010000000
VERSION     DASTKS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684986"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFQ35_02105"
     CDS             complement(<1..255)
                     /locus_tag="VFQ35_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929445.1"
                     /translation="MSSSAARREAPVLAHFPRVVREDLPEIAEQLAEFSERFAGRHVF
                     VTGASGMLAAYLADTLAYLNDVGALSRTCRLTLLVRSAERA"
     gene            complement(252..1607)
                     /gene="rfbH"
                     /locus_tag="VFQ35_02110"
     CDS             complement(252..1607)
                     /gene="rfbH"
                     /locus_tag="VFQ35_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HET9929446.1"
                     /translation="MPNRAAELREQILKLVGEYSALAFQEKPFFPGITPVPVSGRVFD
                     AEEVRALVASSLDFWLTAGPYAATFEQRFARIFGVRGASLVNSGSSANLLALTCLTSP
                     SLGDRRLSPGDEVLTVASGFPTTLNPIIQNRLIPVFVDVDVPTYNVNVAELEAAVGPR
                     TRAIMLAHTLGNPFNLSAVEALAKKHDLYLIEDCCDAVGSLYDGKPVGTFGDVATTSF
                     YPAHHITMGEGGAVLTGSPKLKKIVESLRDWGRDCWCEPGKDDTCGKRYGHKLGELPC
                     GYDHKYTYSHIGYNLKVTDMQAAIGVAQLDKLPAFVAARRQNFALLHEALRELDEHLI
                     LPEPTPNSDPSWFGFPITVRDGAPFTRDELVQHLEHHKIGTRLLFAGNLLRQPAYAGI
                     EHRRASSLETADYITRATFWIGVYPGIDGKKLDYMVSTLTSFVRARRESRRSLAAGAD
                     G"
     gene            1789..>2786
                     /locus_tag="VFQ35_02115"
     CDS             1789..>2786
                     /locus_tag="VFQ35_02115"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9929447.1"
                     /translation="MSANFWQDRPTLVTGATGLLGSWLTRRLVEAGADVVALVRDWVP
                     ESELVLSEHVSRVKIVRGDVTDGALLERVMGEFEIDTVFHLAAQTIVSIANRNPLSTF
                     ETNVRGTWLLLEAARRSPRVRQVVVASSDKAYGSCPTLPYTEDTPLRGEHPYDVSKSC
                     ADLIAQSYAKTYQLPVVVTRCGNFYGGGDLNWNRIVPGTIRSLYRKQAPVIRSDGKYV
                     RDYIHVDDGAAAYMLLAERLAEQRELAGLAFNFSTHERFNVLELVERIRARMMSPLSP
                     VVMGQATGEIVEQSLNADFARQRLGWRPLLSLEQGLDRTIEWYEKFFARDPASRRRP"
BASE COUNT          490 a          890 c          923 g          483 t
ORIGIN      
        1 agcgcgctcg gccgaacgca cgagcagcgt cagccgacac gtgcgcgaca gtgcacccac
       61 gtcgttcagg taggccagag tatcggcgag gtaggcggcg agcatgccgc tcgcgccggt
      121 gacgaacacg tggcggcccg cgaaacgctc ggagaattca gcgagttgct cggcaatttc
      181 cggtaggtct tcccgcacga cgcgcggaaa atgagcgagt acgggcgcct cgcggcgagc
      241 ggcggaggag ctcacccgtc cgctcctgcg gcgagcgagc ggcggctttc acgcctcgct
      301 ctcacgaagc tagtcagcgt gctcaccatg tagtcgagct tcttgccgtc gatgcctggg
      361 tagacgccga tccagaacgt cgcacgcgtg atgtagtcgg cggtctcgag cgacgacgcg
      421 cgacgatgtt cgatgccggc gtaggcgggc tgccgcagta agttgcctgc gaacaagagc
      481 cgggtaccga ttttgtggtg ctcgaggtgt tgtacgagct cgtcgcgcgt gaaaggcgct
      541 ccgtctcgca ccgtgatggg gaagccgaac cagctcggat ccgagttcgg cgtcggctcg
      601 ggcaagatca gatgctcgtc gagctcccgc agcgcttcgt gaaggagagc gaagttctgc
      661 cggcgcgccg cgacgaaggc gggaagcttg tcgagctggg cgacgccaat ggcggcctgc
      721 atatcggtaa ccttcaggtt gtagccgatg tgcgagtacg tgtacttatg gtcgtagccg
      781 cacggcagct cgccgagctt gtggccgtag cgctttccgc aggtatcgtc tttacccggc
      841 tcgcaccagc agtctcgccc ccagtcgcgc aacgattcga cgatcttctt cagcttggga
      901 gagccggtga gaaccgcacc cccctcgccc atcgtgatgt ggtgcgccgg gtaaaagctc
      961 gtggtcgcga cgtccccgaa ggtgccgacc ggctttccgt cgtagagcga accgaccgcg
     1021 tcacagcagt cttcgatcaa gtagaggtcg tgcttctttg cgagcgcttc gaccgctgac
     1081 agattgaatg gattgcccaa cgtgtgcgcg agcatgatcg cgcgcgtgcg cgggcccacc
     1141 gccgcttcga gctcggcgac gttcacgttg tacgtcggga cgtcgacgtc gacgaacacc
     1201 gggatcagtc gattttggat gattgggttc agggtcgtgg gaaagccgct cgcgaccgtg
     1261 agcacctcgt cgcccggcga gagccggcga tcgccgagcg aaggtgacgt gaggcacgtg
     1321 agcgcgagca agttcgcaga agagcccgag ttcacgagcg aagcgccgcg cacgccgaat
     1381 atgcgcgcaa aacgttgctc gaaagtcgcc gcatatggcc ccgcggtgag ccaaaagtcg
     1441 agcgacgaag cgacgagggc gcggacctct tctgcgtcga acacacgccc cgacaccggc
     1501 accggcgtga tccccggaaa gaacggcttt tcttggaagg cgagggcaga atactccccg
     1561 acgagcttca ggatttgttc gcggagctcc gccgcgcggt tcggcatggc cgccgttttg
     1621 caactcacga gccaagtgcg ggcgccgcga tccggccgag ctgcgaagcg cacgtgacga
     1681 cttctcaacg ctcggctgcc agtcgcttga cgccgccgcc gccgcggacg tggttggccg
     1741 cgttatcgag gcgcggcata cggcttgctc ggcgctccca aagagctgat gagcgcgaac
     1801 ttttggcaag accgcccgac gctggtgacc ggagctaccg gccttttggg gagctggctc
     1861 acacggcggc tggtcgaagc cggcgccgac gtcgtagctc tcgtgcgcga ttgggtgccg
     1921 gaaagcgagc tcgtgctgtc ggagcacgtc tcgcgcgtga aaatcgtgcg tggcgacgtc
     1981 accgacggcg cgctgctcga gcgcgtgatg ggcgagttcg aaatcgacac cgtgtttcac
     2041 ctggctgcgc aaacgatcgt ttcgatcgcg aaccgcaacc ctctgtcgac attcgagacc
     2101 aacgtgcgcg gcacctggct cctgctcgaa gccgcgcggc gctcgccccg ggtgcgacag
     2161 gtcgtcgtgg cgtcttctga caaggcgtac ggctcgtgcc ccacgctgcc ctacaccgag
     2221 gatacgccgc tccgcggtga gcatccgtac gacgtgagca aatcgtgcgc ggacctcatc
     2281 gcgcagagct acgcgaagac gtaccagctg cccgtcgtcg tgacccgctg cggcaacttt
     2341 tacggcggcg gtgatctcaa ctggaatcgc atcgtgccgg gcacgattcg ctcgctctat
     2401 cggaagcaag cgcccgtgat tcgctcggat ggcaaatacg tgcgcgacta catccacgtg
     2461 gacgacggcg cggccgccta catgctgctc gccgagcggc tcgcggagca gcgcgagctc
     2521 gctgggctcg ccttcaattt ctcgacccac gagcgcttca acgtgctcga gctcgtcgaa
     2581 cgaattcgcg cgcgcatgat gagcccgctt tctccagtcg tgatggggca agccaccggc
     2641 gaaatcgtgg agcagtcgct caacgccgat ttcgcgcggc agcgcctcgg ctggcggcca
     2701 ctgctctcac tcgaacaagg gctcgaccgc accatcgaat ggtacgaaaa gttcttcgcg
     2761 cgcgatcctg cgtcgcgacg ccgccc
//
LOCUS       DASTKS010000101         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_685042, whole genome shotgun sequence.
ACCESSION   DASTKS010000101 DASTKS010000000
VERSION     DASTKS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685042"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFQ35_02120"
     CDS             <1..588
                     /locus_tag="VFQ35_02120"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929448.1"
                     /translation="EIFSREELVRLFDWERVNKSDGKFDEKKFADVAFEHLKQNELTS
                     LDEYVAWVKPFLSTRGIVEPNEALLRAAIPGIRERARTLADAAVDLEFYFREPPEFDP
                     KAQQKFLTADSGPRLAALRDAIASVEPWNAEALEAAGKRYAETQGIELKAFAQAARVA
                     LTGRSASPPLFEVMAVLGREKTLSRLGRGAELSGG"
     gene            598..1599
                     /locus_tag="VFQ35_02125"
     CDS             598..1599
                     /locus_tag="VFQ35_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9929449.1"
                     /translation="MLAFVGKFADWVGKAPLHSAARALPEVPLTRLNEPSWWAALINY
                     PPIKALIPVPVLAALAPLVWWFFHQTWEELDREATLERARNTEVDYRPAACFVIVAVV
                     LTMQEYYGGRGFYDQVIRPSIESMNLGPVGRARFMRYDELYGYLWWSASRIIGYVLVP
                     LPLWKLLFPKDSLLDMGLRVRGFMSHIWIYGLCLAVVMPLLVVVAYQPDFGSYYPFYK
                     QASRSYVDFAAWELMYFLQFFALELFFRGWFLGALRHSLGSSAIFAMALPYCMIHYGK
                     PYLEANGAIVAGVVLGSLSMRTRSIYAGFLVHITVALSMDFLSLFRRGALPTVLWPV"
     gene            1793..>3274
                     /gene="speA"
                     /locus_tag="VFQ35_02130"
     CDS             1793..>3274
                     /gene="speA"
                     /locus_tag="VFQ35_02130"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET9929450.1"
                     /translation="MNGAKLSASTLDAALDVSRRPPSLEKQNGSSDWTLERAAQRYQI
                     QGWGAPYYHVNARGQIDVTPDPERGQSINLYDLAEELRARGLNLPLLIRFPDIVGHRI
                     KRINESFAKAIAEYDYQGTYRGVFPVKVNQQRHLVEEIVEVGRKWNFGLEAGSKPELL
                     IALACMSDVGGLIICNGYKDRQYIETALLAQKFDKTVIVVLERLEELDIVFRASEKLG
                     IKPLLGVRAKLTARGVGRWADSAGDRAKFGLNSSEIVEVVERLTERNMLDSLQLLHFH
                     IGSQISSIIPIKNAMQEASNIYVELAKMGCRMGYLDVGGGLAVDYDGSKTDFHASKNY
                     QLDEYAADVVAHIHAACTKADIPVPTIVSESGRAIAAHHSVLVFEVVGRNEVRFAEPA
                     EPKPEAHRLLKELYETYRGIQPKNVQESYHDASQAKEEAQSLFKYGYFTLRERSQVER
                     LYWNCCEKIQQTLRRLNFVPEELKHLDRTLSAIYYCNFSLFQSA"
BASE COUNT          600 a         1085 c          989 g          600 t
ORIGIN      
        1 gagattttca gccgcgagga gctcgtgcga ttgttcgact gggagcgcgt caacaagagc
       61 gacggcaaat tcgacgagaa gaaattcgcg gacgttgcct tcgagcacct gaagcaaaac
      121 gagctcacgt cgctcgacga atacgtcgcc tgggtgaagc cgttcctcag cactcgcggt
      181 atcgtcgaac cgaacgaggc gctcttgcgg gcggcgatcc ccggcatccg tgaacgtgcc
      241 cgaacgctcg ccgacgccgc ggtggatctc gagttttact tccgcgaacc gccggaattc
      301 gatcccaagg cacagcaaaa gttcctcacc gccgactccg ggccgcgcct cgcagcgttg
      361 cgcgacgcca tcgcgagcgt cgagccgtgg aacgccgaag ctctcgaggc cgctggcaag
      421 cgctacgccg aaacgcaagg catcgagctc aaggcctttg cgcaggcggc gcgcgtcgcg
      481 ctcaccgggc gctcggcgag cccgcccctg ttcgaggtga tggccgtgct cggccgtgaa
      541 aagaccctct cgcgcctcgg acgaggcgcc gagctatcgg gcggctgagt cgggcgaatg
      601 ctcgctttcg tcggcaaatt cgcggattgg gtcggcaaag ctcccctcca ctcggcggct
      661 cgcgccctgc cggaggtccc actgacgcga ctcaacgagc cgtcctggtg ggcagccctc
      721 atcaattatc cgccgatcaa ggcgctcatc ccggtcccgg tgttggcagc cctggcgccc
      781 ctcgtctggt ggttctttca tcaaacttgg gaggagctag acagagaggc gacgctcgag
      841 cgcgctcgaa acaccgaagt cgattaccgc cccgcggcct gcttcgtcat cgtggcggtc
      901 gtgctcacga tgcaggagta ctacggcggc aggggtttct acgaccaggt gatccgcccg
      961 agcatcgagt cgatgaactt gggacccgtc gggcgcgcgc gattcatgcg ctacgacgaa
     1021 ctgtacggtt acctctggtg gtcggcctcg cgcatcatcg gctatgtcct cgtccccctt
     1081 ccgctctgga agctcttgtt cccgaaggac agcctgctcg acatgggcct gcgcgtgcgc
     1141 ggcttcatga gccacatttg gatctacggg ctctgcctcg cggtcgtgat gccgctcctg
     1201 gtcgtcgtcg cctaccaacc cgacttcggc tcgtactatc cgttctataa gcaggcttcc
     1261 cgcagctacg tcgattttgc ggcgtgggag ctgatgtact tcctgcagtt ctttgcgctg
     1321 gagctgttct ttcgaggctg gttcctagga gccctccgac actcgctcgg ctcctccgcg
     1381 atcttcgcca tggctcttcc gtactgcatg atccactacg gcaagcctta tctggaggcg
     1441 aacggcgcga tcgtcgcggg cgtggtgctc gggtcactct cgatgcgcac gcgcagcatc
     1501 tacgcgggtt tcttggtcca catcactgtg gcgctctcga tggacttcct ctcgctcttc
     1561 cggcgaggcg cgctccccac cgtcctctgg ccggtctgaa tcggcacgaa cgccgctcgc
     1621 gcgcgcagcg ctgagcccga ccgagagaat gatcacttga gcagttgtgg gcgcccgcct
     1681 cgggtggcgt cgaaaggcct cgcgccgcgg cgtcacccaa aaggcaccca cgaggcgcgg
     1741 atcgtccggg tgttgtcggc gaagtcagct atattcggcg cgacgaaaga tcatgaacgg
     1801 cgccaaactc tctgcctcca ccctggacgc ggcactcgac gtgtctcgtc gccccccctc
     1861 tctcgaaaaa cagaacggta gcagcgactg gacgctcgag cgcgccgcgc agcgctatca
     1921 gattcaaggc tggggcgcgc cgtactatca cgtcaacgcg cgcggacaga tcgacgtcac
     1981 ccccgatccc gagcgcgggc aaagcatcaa cctgtacgac ctcgccgaag agctgcgcgc
     2041 gcgcggcttg aaccttccgc tcttgattcg cttccccgac atcgtcggtc accgcatcaa
     2101 gcgcatcaac gaatcgttcg cgaaggcgat cgccgagtac gactaccagg gcacgtaccg
     2161 cggcgtgttc ccggtgaagg tcaatcagca acgccacctc gtcgaagaga tcgtcgaggt
     2221 cggccgcaag tggaacttcg ggctcgaagc gggctcgaag ccggagctac tcatcgcgct
     2281 cgcttgcatg agcgacgtcg gcgggctcat catctgcaac ggctacaaag accggcagta
     2341 catcgaaacc gcgctcctcg cgcagaagtt cgacaagacc gtcatcgtcg tgctcgagcg
     2401 cctcgaagag ctcgacatcg tgttccgcgc ctcggaaaag ctagggatca agccgctgct
     2461 cggcgtgcgc gcaaagctca ccgctcgcgg cgtcgggcgt tgggctgact cggccggcga
     2521 tcgcgcaaag ttcgggctca actcttccga aatcgtcgag gtggtcgagc gcttgaccga
     2581 gcgcaacatg ctcgactcgc tgcagctctt gcacttccac atcggcagtc aaatctcgag
     2641 catcatcccc atcaagaacg ccatgcaaga ggcgtcgaac atctacgtcg agctcgcgaa
     2701 gatgggttgt aggatgggct acctcgacgt cggcggtggt ctcgccgtcg actacgacgg
     2761 gtcgaagacc gacttccacg cgtcgaagaa ctaccaactc gacgagtacg ccgccgacgt
     2821 ggtcgcgcac atccacgctg cgtgcaccaa ggccgacatc ccggttccga cgatcgtgag
     2881 cgagagcggt cgtgccatcg ccgctcatca ttcggtgttg gtgttcgagg tcgtcgggcg
     2941 caacgaggtt cgcttcgccg aacccgcgga gcccaagccc gaagcgcatc gactcttgaa
     3001 ggagctctac gagacgtacc gcggtattca gccgaagaac gtgcaggaga gctaccacga
     3061 cgcgagccaa gccaaggaag aggcgcagag ccttttcaag tacggctact tcacgctacg
     3121 cgagcgctcg caggtggagc gcctgtattg gaactgctgc gagaagatcc agcaaacact
     3181 gcgccgcttg aacttcgtac ccgaggagct caagcacctc gatcgcacgc tctcggccat
     3241 ctactactgc aacttctcgc tgttccagtc ggcg
//
LOCUS       DASTKS010000102         5235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_685059, whole genome shotgun sequence.
ACCESSION   DASTKS010000102 DASTKS010000000
VERSION     DASTKS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685059"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFQ35_02135"
     CDS             complement(<1..1380)
                     /locus_tag="VFQ35_02135"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET9929451.1"
                     /translation="MSVAVSIASPLTLDFDNSFVRELPGESGPPHTPRQVRGACWSHV
                     PPTPVKGPRLLAHSREVAELVGIPEGAIASPEFAGVFGGNALLPGMQPYSACYGGHQF
                     GNWAGQLGDGRAITLGEVVNARGERWELQLKGAGRTPYSRTADGRAVLRSSLREFLLS
                     EAMHHLGIPTTRALCLVETGESVIRDMLYDGHPRAEPGAIVCRVAPSFVRFGNFEIFA
                     SRDELPLLKQLTDYTLRTHYPELGTPSKDAYLALLREVSRRTAQLMVHWMRVGFVHGV
                     MNTDNMSILGLTIDYGPYGMLDDFDPNFTPNTTDAHGRRYRYANQPLIARWNLQRLAE
                     ALLPLIADVRAVEAALETYAEAYEAEQHIVLAAKLGLAPFKNDSAMAAEDPASDGAFV
                     DGLFEVLAGVETDFTLFFRCLATLPLENSNDEAAWLAALRPAYYDASPLPETARLRTL
                     AWLRNYAERA"
     gene            complement(1441..1986)
                     /locus_tag="VFQ35_02140"
     CDS             complement(1441..1986)
                     /locus_tag="VFQ35_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929452.1"
                     /translation="MQGSSWRVGLGIAVACSACDEIPSETVGRASTALGADCADAIPL
                     QTFTGTVDYVAVPPYTASGCENASVVRVDSILRSEHSGGGGGANSPWGSKVEFSVVYN
                     HKTYGPCAPSLAAYAFLHDTTNETWIPGKHGTTSAFPVSDNSPCPALSLELDVVSGKD
                     FKFAFAGKNDDQTLVRIRLLP"
     gene            complement(2065..3087)
                     /locus_tag="VFQ35_02145"
     CDS             complement(2065..3087)
                     /locus_tag="VFQ35_02145"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HET9929453.1"
                     /translation="MSSVLRPARLLDPGACLALRPLKYPAFYDMYRNAVRNTWTVDEV
                     DFSKDVDDIHRRLNPGEKHLIERLVAFFATGDSIVSNNLVLNLYKHVNAPEARMYLSR
                     QLYEEALHVEFYLTLLDNYVPDPVQRAQAFAAVEHVPSVAHKANFCFRWIDSVHGLNR
                     LDLRSERRSFLRNLIAFAACVEGLFFFGAFAYVYYLRSRGLLHGLATGTNWVFRDESA
                     HMAFAFEVVRIARQEEPELFDAEMERDVRSMLREAVDGETLFAQDLVGNGLPGLSLRD
                     VRGYLEFVADQRLATLGLSPEFRSKNPLSFLELQDVQEVTNFFERRVASYQVGVSGEV
                     VLDGGF"
     gene            complement(3084..>5235)
                     /locus_tag="VFQ35_02150"
     CDS             complement(3084..>5235)
                     /locus_tag="VFQ35_02150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET9929454.1"
                     /translation="LDELSIQTAAGLIVDQPEYSQLAARLLATYVDKEVAGQDVHSFS
                     QSIAFGHRLGIINDRLLEFVTRAARKLNDAVDPSKDQLFEYFGLRTVYDRYLLRHPST
                     RRVFETPQYFFLRVACALSQTVMEALSLYRLISNLEYLPSSPTLFNAGTRHEQLSSCF
                     LLDSPRDHLEDIYKKYADVAALSKFSGGIGISYTRVRSRGSLIESTNGHSSGIVPWLK
                     TLDASVAAVNQGGKRKGACAVYLEPWHADIEEFLELRDGTGDHQRRTHNLNLANWLPD
                     LFMQRVEADAQWSLFDPKTVPDLVDTWGEAFESAYLAAESAGTAVRSVKARDLYARMM
                     KTLAETGNGWICFKDKSNRACNQTGNGGTVHLSNLCTEILEVTSNDETAVCNLGSLNL
                     VRYVENGAFDFEKLARSVHIAVRHLDRVIDLNYYAIPSARSSNQRWRPVGLGIMGLQD
                     LFFELRLPFDSDEARALSRRIAEEIYFHALQTSCDLAEELGAHAAFAETRAARGELQF
                     DAWGVAPENQERFAALAERIQRVGLRNSLLIAIAPTATIAAIAGTSECIEPQVSNLFK
                     RETLSGEFLQVNRWLVRDLEALGLWNEAVRDKLKVAEGSVQGFQELSPELRHLYRTAW
                     EVPMRALIDMAADRGAFIDQSQSLNLFVETPNIGRLSSMYFYAWKRGLKTTYYLRSRP
                     ATRIAKATVAAPAEEAVQAVSCSLENPETCEACQ"
BASE COUNT          978 a         1561 c         1766 g          930 t
ORIGIN      
        1 cgcgcgctcg gcatagttgc ggagccacgc aagagtgcgg aggcgcgctg tctctgggag
       61 cggtgatgcg tcgtagtagg cgggacgcag ggctgcgagc caggcggctt cgtcgttcga
      121 attttcgagc gggagagttg ccaagcagcg gaagaagagc gtgaagtcgg tttcgacgcc
      181 ggcgaggact tcgaagagcc cgtcgacgaa ggcgccatcg ctcgcggggt cttcggccgc
      241 catggcgctg tcgtttttga aaggagcaag gccgagcttc gcggcgagga cgatgtgctg
      301 ctcggcttcg taggcctcgg cataggtctc gagcgcggcc tctacggcgc ggacgtcggc
      361 gatgagaggg agcagggctt cagcgaggcg ctgcaggttc cagcgcgcga tgaggggttg
      421 gttcgcgtag cgataacgcc ggccgtgcgc gtcggtggtg ttgggggtga aattcggatc
      481 gaagtcgtcg agcatgccgt acgggccgta gtcgatggta aggcctagta tcgacatgtt
      541 gtcggtgttc atcacgccgt ggacgaagcc gacgcgcatc cagtgcacca tgagctgcgc
      601 ggtgcggcgc gagacctctc gcagcaacgc gaggtaagcg tctttcgacg gagtgccgag
      661 ctccggatag tgcgttcgca gcgtgtagtc ggtgagctgc ttcagcagcg gaagctcgtc
      721 gcgcgatgca aagatttcga agttgccgaa gcgcacgaac gacggtgcga cgcgacacac
      781 gattgcaccc ggctccgcac gcggatgccc gtcgtagagc atgtcgcgga ttacgctctc
      841 gcccgtttcg accaggcaga gcgcgcgggt cgtcggaatg ccgaggtggt gcatcgcctc
      901 gctcagcaag aactcgcgca gcgacgagcg cagaaccgca cgaccatccg cggtgcgaga
      961 gtacggcgtg cgaccggcgc ctttgagctg gagctcccag cgctcgccgc gcgcgttgac
     1021 gacctcgccg agcgtgatgg cacgcccgtc accgagctgt cccgcccaat tgccgaactg
     1081 gtgcccgccg taacacgcgg agtacggctg cattccgggc aacagcgcgt tgccaccaaa
     1141 gacccccgcg aattctggcg aagcgatggc tccctccgga atacccacca gctcggcgac
     1201 ttcgcgtgaa tgcgcgagca ggcgcggccc tttcaccgga gtcggaggga cgtgcgacca
     1261 gcaggcgccg cgcacctggc gcggtgtgtg cggcgggccg gattcacccg gcaattcgcg
     1321 cacgaagcta ttgtcgaaat cgagcgtgag aggggacgcg atggaaacgg cgacggacat
     1381 gcgcgagagc gcagtgtagt ccgccggccc gatgccgcta gctacggtac cgttcggagg
     1441 ctaaggaagg aggcgaatgc gcacgagtgt ttgatcgtcg ttctttccgg cgaaggcgaa
     1501 cttgaaatct ttcccgctca ccacgtcgag ctcgaggctg agggccgggc aaggcgagtt
     1561 atccgatacg gggaacgcag aggtcgtgcc gtgcttgccg gggatccagg tttcgttggt
     1621 cgtgtcgtgc aagaaggcgt aggcggcaag actcggggcg caggggccat atgtcttgtg
     1681 gttatagacc acagagaatt caaccttgct gccccagggg gagttcgctc cgccgccacc
     1741 tccgctgtgt tccgaacgca ggattgagtc tacgcggacc accgaagcgt tctcgcatcc
     1801 agacgccgtg tatgggggga cagcgacata gtcgaccgtg ccggtgaacg tctgcaacgg
     1861 tatcgcgtca gcgcaatcgg caccgagcgc cgtgctcgct cttccgaccg tttctgaagg
     1921 aatctcgtcg catgcggagc acgctactgc gatccccaag ccaactcgcc acgaagaacc
     1981 ttgcatctca cacctccagg cactcgaggc tatcgtccaa cttcgggcgt cgcaaggggg
     2041 agcgagaagg gtatccgagg ctcactaaaa gccgccgtct agtacgactt cgccgctcac
     2101 gccgacttga tacgacgcga cccggcgctc gaagaagttc gtgacctctt ggacgtcttg
     2161 tagctccagg aacgaaagcg gattcttgct gcgaaactcg ggggaaaggc cgagcgtcgc
     2221 aaggcgctga tcggccacga actcgaggta gcctcggacg tcgcggagcg agagccccgg
     2281 caggccattg ccgacgagat cttgcgcgaa cagggtttcg ccatcgaccg cctcgcgcag
     2341 catgctgcgc acgtcgcgct ccatttcggc gtcgaagagt tcgggctcct cttgccgcgc
     2401 gatgcgcacc acttcgaacg caaaggccat gtgcgcgctc tcgtcgcgaa aaacccaatt
     2461 ggtgccggtg gcgagcccgt gcagcaggcc tcgtgaacgg aggtagtaaa cgtacgcaaa
     2521 agcaccgaag aagaagagcc cttcgacgca cgccgcgaac gcgatgaggt tccgcaagaa
     2581 ggagcgacgt tcgctgcgaa ggtcgagccg attcagacca tgaacgctgt cgatccagcg
     2641 gaagcaaaag ttcgccttgt gcgcgaccga cggcacatgc tcgaccgcgg cgaacgcttg
     2701 cgcacgctgg acggggtcgg gcacgtagtt gtcgagcaac gtgaggtaga actcgacgtg
     2761 tagcgcctct tcgtagagct gtcgcgacag gtacatgcgc gcttcgggcg cgttcacgtg
     2821 cttgtagagg ttcagcacca ggttgttcga gacgatgctg tcccccgtcg caaagaaggc
     2881 gacgagccgc tcgatcaggt gcttttcacc ggggttgagg cggcggtgaa tgtcgtccac
     2941 gtctttgctg aagtcgactt cgtccaccgt ccacgtgttg cgcacggcgt tccggtacat
     3001 gtcgtaaaac gccgggtatt tgagcggtcg cagcgcgagg caagctccgg ggtcgagcag
     3061 gcgcgcgggg cggagaacgg agctcattgg catgcctcac aggtttcggg gttctcgagc
     3121 gagcacgaca ccgcctggac ggcttcttcc gcaggcgccg cgacggtcgc cttggcgatg
     3181 cgcgtggctg gacgcgaacg caggtaatac gtcgttttga ggccgcgctt ccaagcgtag
     3241 aagtacatcg aggagaggcg accgatgttc ggcgtctcga cgaagaggtt gagcgattgg
     3301 ctctggtcga tgaaggcgcc gcgatctgcc gccatgtcga tgagagcgcg catcggcacc
     3361 tcccacgccg tgcggtacaa atgccggagc tccgggctca gctcctgaaa gccctgaacg
     3421 gaaccttcgg cgaccttgag cttgtcgcgc acggcttcgt tccagagccc gagcgcttcg
     3481 agatcgcgca ctaaccagcg gtttacctgc aggaattcgc cggacagggt ctcgcgtttg
     3541 aacaggttcg agacttgcgg ctcgatgcac tcggaggtgc cagcaatcgc ggcgatggtg
     3601 gcggtaggcg ctatcgcgat gagcagcgag ttgcgaagcc cgacgcgctg aatccgttcc
     3661 gcgagcgcgg cgaagcgctc ctggttttcg ggcgcgacgc cccacgcatc gaactggagc
     3721 tcgccgcgcg cggcgcgtgt ctcggcgaag gcggcgtgcg cgccgagctc ttctgccaag
     3781 tcgcaagaag tttggagcgc atgaaagtag atctcttccg caatgcgccg cgaaagcgcg
     3841 cgcgcttcgt ccgagtcgaa cggaaggcgc agctcgaaga agagatcttg caagcccatg
     3901 atgccgaggc cgaccgggcg ccagcgctgg ttcgagcttc gagcgctcgg gattgcgtag
     3961 tagttgaggt cgatgacgcg atcgaggtga cgcacggcga tgtgcacgct gcgcgcgagc
     4021 ttctcgaagt cgaaggcgcc gttctcgaca tagcgcacca ggttcaacga accgaggttg
     4081 cacaccgcgg tttcgtcgtt cgacgtcacc tcgaggattt cggtgcacag gttcgagaga
     4141 tgcacggtgc cgccgtttcc ggtctggttg caggcccggt tcgacttgtc tttgaagcaa
     4201 atccagccgt ttccggtctc ggcgagtgtc ttcatcatgc gcgcgtagag atcgcgagct
     4261 ttcacgctgc gcaccgcggt gccagcgctc tccgcggcga ggtaggcgct ttcgaaagct
     4321 tcgccccagg tgtcgacgag gtcgggcacc gtcttcgggt cgaacaggct ccactgcgcg
     4381 tccgcttcga cgcgctgcat gaacaagtcg ggtagccaat tcgcgagatt caggttgtgg
     4441 gtgcggcgct ggtgatcgcc cgtgccgtca cgcagctcca agaactcttc gatgtcggca
     4501 tgccacggct cgaggtacac cgcgcacgcg cctttgcgct taccgccctg gttcacggcc
     4561 gcgaccgaag cgtcgagcgt cttcagccac ggcacgatgc cgctcgagtg tccgttggta
     4621 gactcaatca gcgagccgcg cgagcggacg cgcgtatacg agatgccgat gccacccgag
     4681 aacttcgaga gcgccgcgac gtcggcgtac ttcttgtaga tatcctcgag atggtcccgc
     4741 ggcgaatcga gcaagaagca agacgagagc tgctcgtggc gagttccggc gttgaatagc
     4801 gtgggggagc tcggcaggta ctcgaggttc gagatcagcc ggtacaacga gagcgcctcc
     4861 atcaccgtct ggctgagcgc gcaggcgacg cgaaggaaga aatattgcgg cgtttcgaac
     4921 acgcggcgcg tcgacggatg ccgcaacaaa tagcggtcgt acaccgtgcg caacccaaag
     4981 tactcgaaca gctggtcttt gctcggatcg accgcgtcgt tcagctttcg cgcggcgcgc
     5041 gtcacgaact cgagcaagcg atcgttgatg atgccgaggc gatgtccgaa cgcgatcgat
     5101 tgggaaaacg agtgcacgtc ttggcccgcg acttccttgt cgacgtacgt cgccaacagg
     5161 cgcgccgcga gctgcgaata ttccggttga tcgacgatga gccccgccgc cgtttgaatc
     5221 gagagctcgt cgagc
//
LOCUS       DASTKS010000103        14356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_16786,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000103 DASTKS010000000
VERSION     DASTKS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14356
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16786"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="VFQ35_02155"
     CDS             complement(<1..2281)
                     /locus_tag="VFQ35_02155"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HET9929455.1"
                     /translation="MWQSLPEFGTGVIYAVLVAAAYTFAISLAAGRGQPRLLRAARLG
                     AYATSALVLFSVLLLTYAFVTHDFRIRYVSHYSDRSMPKLYLITSLWGGQDGSLLWWS
                     LLLSGYTAGAVAWMKGRYRQLQPYVIATLMVITSFFMVLMLFAANPFEASVAGTRPDG
                     DGLNPLLQNYWMVIHPPCLYTGFVGCAVPFAFCVAALVTGRLDNEWINAVRKWMLFAF
                     MFLSIGNVLGMAWAYEELGWGGFWAWDPVENAACLPWFTAAAYVHSTMIQERRNMLKV
                     WNVFLISLTFLLTLFGTFLTRAGIITSVHSFAQSDIGKYFLWFIGIVAATSIGLSVWR
                     ARELRSRAQIESVASREAMFVVNNWALLGGMTFIFCATMYPKLSELWGENTTVGPSFF
                     NRWMAPIGLVIFALMGLAPLFGWRKTSDDALKRSTRGPLITFAVAFALHVAIGRSLGY
                     PAFVEKDPMFGGFLGTVLQKIASSGPVITISLAAFNVAVIVQEFYRGIVARQNASEKK
                     GEREGGLAAFLNLVDKSRRRYGGYIVHLGITCMFIGFVGTAWNIDREASMTPGQTTTL
                     GRFTLVYKGSRMCPGNPRCSPEEQSDLNKRMIFADLDVLRDGKPVGMVSPAKFIYHKS
                     PESPTTEVALMRSFREDLYTIVGTIDPQSKRATFKFHVNPFVSWIWVGLLVLIGGASI
                     SLWPEVAFSSSRAWSFARAGAGVATGTMLSVWLAMTPATAYAKERPRSHPTFSVASPL
                     PRLFDSGAWRPLALGLLLGT"
     gene            complement(2384..3115)
                     /gene="pyrH"
                     /locus_tag="VFQ35_02160"
     CDS             complement(2384..3115)
                     /gene="pyrH"
                     /locus_tag="VFQ35_02160"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9929456.1"
                     /translation="MEPDPKLKFRRVVVKLSGEVLSGKEGGQGIDTATLKATANELAE
                     VHATGVQIGIVVGGGNIFRGLRAASEGMDRAQSDYMGMLATVINSLALQDALERSGVP
                     TRVMTAIEIGKVAEPYIRRRAMRHLERGHVVVFGAGTGNPYFSTDTAAALRAMEIRAD
                     GLLKATKVDGIYDRDPRRHPDAKMLERVSYDRFLADHLGVMDSTAVTLCRENGLPIRV
                     FKMTPGNIRRVCLGENVGTTVSLSS"
     gene            3208..4272
                     /locus_tag="VFQ35_02165"
     CDS             3208..4272
                     /locus_tag="VFQ35_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9929457.1"
                     /translation="MLLARARRKALSFVASCTLLAAFASACGKRDRGVAPVPSSSAVP
                     EAPTAKALTPEQAQQVLAQVGDVKITLGDYAASLERMDSFERLRYQSPERRRLLLNEM
                     IDLELLAQEARRRGLDQQPETQERLRQLLREELLRQVRKEVPAPNDVAPADVRKYYDE
                     HKADFDEPERRRVAHISVASRAQAEALLEKAKNASAADWGKLVVDNSLDKGSRAFGAA
                     PPELAGDLGIVGPPGHARGANPRVPEPLRTAAFKIDKVGSVYPEVVADGGVFHIVRLT
                     SRTDARERTYEAAERTIRISIVQELVKQREASFEKELRAKYPVNVDEAALAKVHVPKP
                     SPSAGAGVVLPTPLPGRARP"
     gene            4269..5354
                     /locus_tag="VFQ35_02170"
     CDS             4269..5354
                     /locus_tag="VFQ35_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET9929458.1"
                     /translation="MIELAVPGDWRWQLRHAVRSVAELERHLSLTPEELEGARHAEDQ
                     GFPLAITPYYLSLCDREDPNCPIRRQVVPLAAEQRSVAGDLRDPLGEEAHEVAPELVQ
                     RYPDRALLLVSDRCAVYCRFCTRSRMVGDGGGPRSDARLAAAFTYLETHPEVRDVIVS
                     GGDPLTMATERLLRIIERLRTIPSVETIRLATRVPVALPQRITDELLGALKPHHPLWV
                     MTHFNHPKELTEESRAALCRLADNGFPVMNQTVLLRGVNDDSRVLTELFRGLVKVRAR
                     PYYLLQADPVRGSGHLRTPLATGIRIAEELQGRLSGIALPKFIVDTPGGFGKVPVGPE
                     WVVHRAPGVTRFRTPRGVEVDYVDPPE"
     gene            complement(5367..6926)
                     /locus_tag="VFQ35_02175"
     CDS             complement(5367..6926)
                     /locus_tag="VFQ35_02175"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9929459.1"
                     /translation="MSEPKSRPSLDALAERDARALNAGGTERVKKQHAAGKLTARERI
                     DALLDPGSFVEIGRLVAQRHHEFGAEQAPIPGDGVVTGHGTVEGRPVFVYAQDFTVFG
                     GSLGTAHAEKIVRLMDHALRLGAPVVGLCDSGGARIQEGVASLAGYADLFLKNTLASG
                     VVPQISCIMGPCAGGAVYSPAITDFIFMVENTSYMFITGPDVIRAVTHEEVTKEALGG
                     ASAHAAKSGVCHVTARDDYACLAQVRELLSYLPQNNTEDPPRHATNDPSEREIPELDN
                     LIPEESHKPYDMGALIKKVVDDERFYEIHAEFARNIIVGFARIGGQPVGIVANQPLVL
                     AGVLDIDASIKAARFVRFCDCFNIPIVTWVDVPGFLPGVEQEHRGIIVHGAKLLYAFA
                     EATVPKLTVITRKAYGGAYDVMSSRHIRADVNLAFPTAEIAVMGPEGAVNIVYRSAIE
                     SAPDKEAARREFIEDYRARFANPYKAAELGFVDEVIRPREVRTRLATYLRLLRNKRDK
                     NPPRKHGNIPL"
     gene            complement(6992..8347)
                     /locus_tag="VFQ35_02180"
     CDS             complement(6992..8347)
                     /locus_tag="VFQ35_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929460.1"
                     /translation="MKGRKSEPGFEIPDLDLPPPKPSASQARLAAVRFSESGERQRAP
                     MVTGLSELPAGPPLDLDLNSPISSVRGSEIPAHEVTGRAGFQLDSMLPEVAGITVAPE
                     GRANWPSGVTEERALLEFDPKQVEALARYGSPSSLGVLNVLYALRVFAQRRRLRRELL
                     QCEAELARVESARDDLLARLAEQKREALSGNPGFQRFVHEVAEMDAQGSARAQLQNAA
                     AQEQAAELNRLDAELSTVLPELRSAESALGELSAVQAEREQTLLRLEARHKRGFIELR
                     AMEQRGAGVEEAEHVRAQQQALVPQLEAAKAAYEATLGELAVRAKHQREVRYRVNEIE
                     RKKRDLRARFQQRLDSKKKEFGAVEKRQHRALADLGRAVLAARGGIPLEPALLGPLRD
                     ADAAVSRALAESEMRVRALDACDRDKLATGYSWMWAIAAAIVAIVCYRSFVRAESPTL
                     H"
     gene            8470..8557
                     /gene="ffs"
                     /locus_tag="VFQ35_02185"
     ncRNA           8470..8557
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFQ35_02185"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8617..9312)
                     /locus_tag="VFQ35_02190"
     CDS             complement(8617..9312)
                     /locus_tag="VFQ35_02190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9929461.1"
                     /translation="MSEPLLAVVTGTSRGIGRATALSLSKRGLRLVAITRDAEDRSGL
                     REACRAVGAPSVDIVPLDLNDLDSVERVGADVAGRFGAPAVVIHNAGEVVRARVDELS
                     RAEWMRQLNVNLSAPFVLTRALLPHMLEKRRGRIVFVASISSSIGTPRHSAYCASKWG
                     IVGFMKSLAAELTDSGLMTAAVLPGSVDTRMLEGSGFPPRMSAEDVAHTLTHFALDAP
                     LAHNGAVIEMFGV"
     gene            complement(9309..9887)
                     /locus_tag="VFQ35_02195"
     CDS             complement(9309..9887)
                     /locus_tag="VFQ35_02195"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET9929462.1"
                     /translation="MRAPDLKAAAQGIAIFLRALGYEERSELVGTPDRVAEAFAHELL
                     RGERVDVAELLREGSEPATAATSGLVVVRDIAVVAVCPHHLLPAVGRATVAYLPGERL
                     LGLGTVARLAQACSQRLALQEAIGDAVVEALLTHARARGAFCRLSLLHTCLATRGAEQ
                     PDARLVTIARGGSLATPDGALELSLALGEAAK"
     gene            complement(9933..10370)
                     /locus_tag="VFQ35_02200"
     CDS             complement(9933..10370)
                     /locus_tag="VFQ35_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9929463.1"
                     /translation="MDSARVFTISEVNALIPTLSVFVAQQLREQSNIEQGLAELTRLE
                     GETPNSLEHREGDSAEVRRLKADLRRRVGRYEAGWSKVRELGGVVKDPQIGLIDFYGR
                     VDGRMVWLCWRYGEDTLGYYHELGGGYFRRRPLRADVRKGLLN"
     gene            complement(10385..10903)
                     /locus_tag="VFQ35_02205"
     CDS             complement(10385..10903)
                     /locus_tag="VFQ35_02205"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9929464.1"
                     /translation="MSDVREADSQSEPARGLLTTGEMARRTNSTLRTVRFYEEAGLLC
                     PTARAEGGHRLFPESELEKLKLASELRAAGLSLPEIKHLFAIKREATSGADAAAAIDG
                     ALERYAERINERMALLQRLAIELESARSVARRCRACTDNHLFPESCGECRTMKDAGVL
                     TPAATVLWDLKR"
     gene            complement(11196..12056)
                     /locus_tag="VFQ35_02210"
     CDS             complement(11196..12056)
                     /locus_tag="VFQ35_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929465.1"
                     /translation="MTISSPNSSDGADRPSRTSLAPPPPPTWAARRVDAGIAFFSHLG
                     VIAEMTVRTLRAGFRRPFEWKAILTQLEVLGVASVGIVTVTSVFIGMVMATQFAFGLR
                     KFGGMEYTGRVIALSFARELAPTLTAVIVGGRIGSGMAAEVGAMSVTEQIDAVRALGA
                     DPLKKLVWPRLVASVIVMPMLGAFALVLGFTGAMLITDVQFDIPSAFFLRTALSAVTL
                     ADFVSGMLKTPFFGAIIALVGCHFGMTTTGGTAGVGNSTTRTVVAISIAILIADFFLT
                     KVGIIFWPTG"
     gene            complement(12145..12654)
                     /locus_tag="VFQ35_02215"
     CDS             complement(12145..12654)
                     /locus_tag="VFQ35_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929466.1"
                     /translation="MKARFLALGLLAWALAATPGCGGPTFDGKVYRSSEIAFRVGPVP
                     QTWRSIEVEGAALSFRDDPSGATVAVNGRCGVDGDDVPLAALTHHLFLNFTNRVVGSQ
                     KELVIDGRGALRTELDAHLDGVPKHFVVYVLKKDGCVYDFMWIGAEGAPAASVEDFDH
                     FVAGFSTST"
     gene            12776..13528
                     /locus_tag="VFQ35_02220"
     CDS             12776..13528
                     /locus_tag="VFQ35_02220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9929467.1"
                     /translation="MLPISDQNPTRTFPFVNYLLIALNVLVFGWQYLLFSTGGEAAVS
                     GLGLVPARFSQNPVGELGNLFASMFMHGSLGHIAGNMLYLYIFGDNVEDAMGHFRYIV
                     FYLACGFIAALAQVVVAPMSPLPMVGASGAIAGALGGYILLYPRAPVTVLNPILPLWL
                     LFGLFFQLPAWLAIGIWFVPNILNALVEESTAGGIAFFAHIGGFVAGMLLVRVATRGR
                     RRIDRGRWSGWRAPPRRPTRGSWSANTSRSWY"
     gene            13542..>14356
                     /gene="cysS"
                     /locus_tag="VFQ35_02225"
     CDS             13542..>14356
                     /gene="cysS"
                     /locus_tag="VFQ35_02225"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9929468.1"
                     /translation="MPLVLYNTLTRNNEELNPKAGRVKMYCCGPTTYDVPHVGHARAA
                     LVPDILVRRLRDLGLDVTYVRNITDVDDKILARAKQNGEQPLALSHRMALVYQEQMAM
                     VGCEPPTHEPRVSENIAEICSLIERLIANEAAYVVDMPGGKTDVYFAVRKFESYGKLS
                     RRRIDDLVVGARVAEGENKRDPLDFALWKGTGPEDWGWDSPWGRGRPGWHIECSAMSG
                     RYLGHGFDVHTGGMDLIFPHHENEIAQSESLTGKPFVRYWVHNGMLQLGGEKMS"
BASE COUNT         2473 a         4669 c         4777 g         2437 t
ORIGIN      
        1 cggttcctag caaaagtcca agggcgaggg gacgccaggc gccggagtcg aacaagcgag
       61 gcagcggaga ggccacgctg aaggtcgggt gtgaacgcgg gcgctctttt gcgtaggcgg
      121 tcgcaggcgt catcgcgagc caaaccgaga gcatggtgcc ggtagcgacg ccagcgccgg
      181 cgcgcgcgaa cgaccaagcg cgagaactcg agaacgcgac ttcgggccaa agagaaatcg
      241 aggcgccgcc gatcaagacc aagagaccca cccaaatcca agacacgaat ggattcacgt
      301 ggaacttgaa ggtggcgcgc ttgctctgcg gatcgatggt cccgacgatc gtgtagaggt
      361 cttcacggaa cgagcgcatg agcgcgacct cggtcgtcgg gctctcgggg ctcttgtggt
      421 agatgaactt cgcgggcgac accattccga ccggcttgcc gtcgcgcaag acgtcgaggt
      481 cggcgaagat catgcgcttg ttcaggtctg actgctcttc ggggctacag cgcgggttgc
      541 ccgggcacat gcgcgagccc ttgtagacca gggtgaagcg gccgagggtc gtggtctgac
      601 cgggcgtcat cgaggcttcg cgatcgatgt tccaggcggt gccgacgaag ccgatgaaca
      661 tgcacgtgat gccgaggtgc acgatgtaac cgccgtagcg gcggcggctc ttgtcgacca
      721 agttgagaaa tgcggcgagg cctccctcgc gttcgccctt cttttcggag gcgttctggc
      781 gggcgacgat gccgcgatag aactcttgca cgatgaccgc gacgttgaag gcggcgagcg
      841 agatggtgat gacggggcca ctcgaggcga ttttctgcaa tacggtgccg aggaagccgc
      901 cgaacatcgg gtccttctcg acgaacgcgg ggtagccgag gctcctgccg atcgcgacgt
      961 gcagcgcgaa ggccacggca aaggtgatga gcggaccgcg cgtcgacctc ttcagagcgt
     1021 cgtcgctcgt cttgcgccaa ccgaagagcg gggcgagccc catcagcgcg aagatcacga
     1081 ggccgatcgg cgccatccag cggttgaaga acgaagggcc gacggtcgtg ttctcgcccc
     1141 agagctcgct gagcttcggg tacatcgtcg cgcaaaagat gaaggtcatg ccgccgagca
     1201 aggcccagtt gttcacgacg aacatcgcct cgcggctcgc gacgctctcg atttgagcgc
     1261 ggctccggag ctcgcgcgcg cgccaaacgg agaggccgat gctcgtcgcc gcgacgatgc
     1321 cgatgaacca gaggaagtac ttgccgatgt cgctctgcgc gaagctgtgc acgctggtga
     1381 tgatgccggc gcgggtgagg aacgtgccga acagcgtgag caggaacgtg agcgagatga
     1441 ggaacacgtt ccagaccttg agcatgttgc gccgctcttg gatcatcgtg gagtgcacgt
     1501 acgccgcggc cgtaaaccag ggcaaacacg ccgcgttctc caccgggtcc cacgcccaga
     1561 aaccgcccca accgagctcc tcgtaagccc acgccatgcc gagcacgttc ccgatcgaga
     1621 ggaacatgaa cgcgaagagc atccacttgc ggaccgcgtt gatccactcg ttgtcgagtc
     1681 gacccgtcac cagggcggcg acgcaaaacg cgaacggcac cgcgcacccg acgaaccccg
     1741 tgtagagaca gggcgggtgg atgaccatcc agtagttttg cagcagcgga ttcaggccgt
     1801 cgccgtcggg ccgagtgccg gcgacgctcg cctcgaaggg gttcgccgcg aacagcatca
     1861 gcaccatgaa gaagctggtg atgaccatca gcgtggcgat gacgtagggc tgcagctgcc
     1921 gataacggcc tttcatccag gccaccgcgc ccgcggtgta accggagagc aacagcgacc
     1981 accagagcag cgagccgtcc tgaccacccc acaaagaggt gatgaggtag agcttcggca
     2041 tcgaccggtc gctgtagtgc gacacgtaac ggatgcgaaa gtcgtgcgtg acgaacgcgt
     2101 acgtgagcag cagcaccgag aagagtacga gcgccgaggt cgcgtaggcg cccagccgcg
     2161 ccgcccgcaa gagccttggt tgaccgcgac ccgctgcgag cgaaatcgcg aaggtgtaag
     2221 ccgccgccac gagcacggcg tagatcacac ctgttccgaa ttcagggagc gactgccaca
     2281 tcggggcgga gcttaccccc aaaatctccc caagccgaca cacacccaat cttccggccc
     2341 cgcggttgga attttcccac cgcggagcgc aggaaggagt cgctcagctc gacagcgaaa
     2401 cggtggttcc cacgttttcg ccgagacaga cgcggcggat gttgccgggt gtcatcttga
     2461 aaacgcgaat gggaaggccg ttctcgcggc acaaagtgac tgcggtcgag tccattaccc
     2521 cgaggtgatc ggcgaggaag cggtcatagg acacgcgctc gagcattttc gcatcggggt
     2581 gccgtcgcgg gtcgcgatcg taaatgccgt ctaccttcgt ggctttgagc aaaccgtcgg
     2641 cgcgaatttc catcgcgcgg agcgccgcgg cggtgtcggt ggagaagtag gggttgccag
     2701 tgccggcgcc gaagacgacg acgtggccgc gctcgaggtg acgcatcgcg cggcgacgaa
     2761 tgtacggttc cgccaccttt ccgatttcga tggccgtcat cacgcgggtc ggcacgccgc
     2821 tgcgctcgag cgcgtcttgc agagcgagcg agttgatcac ggtcgcgagc atgcccatgt
     2881 agtcgctctg ggcgcggtcc atgccctcgc tggcagcgcg caaaccgcgg aaaatattgc
     2941 cgcccccaac tacgatgcca atttgtacgc cggtcgcatg gacctcggcg agttcgttgg
     3001 cggtggcttt gagtgttgcg gtgtcgatgc cctggccgcc ttctttgccg ctcagcacct
     3061 cgccgctgag cttgacgacc acacgtcgga atttgagctt tggatcgggt tccacgatcg
     3121 attcccgtac ccttcccttc cccggttttc aacgccgaag gcgggtttcc ttggctaagc
     3181 gcgagatgcc gtagtaatcc gagccgcatg ttgctcgctc gggcgaggcg taaagcgctc
     3241 tctttcgtgg cctcttgcac gcttctcgct gcgtttgcga gcgcttgcgg caagcgcgac
     3301 cgcggtgtgg cacccgtgcc gagctcgagc gccgtaccgg aggccccgac cgcgaaagcg
     3361 ctgacgccgg agcaggccca gcaggtcctg gcccaggtcg gcgacgtgaa gatcacgctg
     3421 ggggactacg ccgcgtcgct cgagcgcatg gatagcttcg agcgcctccg ctaccagtcg
     3481 ccggagcggc gccggctgct cttgaacgag atgatcgatc tcgagctttt ggcgcaagag
     3541 gcgcggcgcc gcgggctcga ccagcagccc gagacgcaag agcggcttcg tcagctgctg
     3601 cgcgaagagc tgctgcgtca ggtgcggaaa gaggtgcccg cgccgaacga cgtggcgccc
     3661 gccgacgtgc gaaagtacta cgacgagcac aaggccgact tcgacgagcc ggagcgccgg
     3721 cgcgtggctc acatcagcgt cgcgagccgg gcgcaagccg aggcgctgct cgaaaaggcg
     3781 aaaaacgcga gcgccgccga ttggggcaag ctcgtggtcg acaactcgct cgacaagggc
     3841 tcgcgggcgt tcggggccgc gccgcccgag ctcgcgggtg acttgggaat cgttgggccg
     3901 ccaggacatg cacgcggcgc gaatccgcgc gtgccggagc cgctgcggac ggcggcgttc
     3961 aaaatcgaca aggtcggctc cgtgtatccg gaggtcgtcg ccgacggagg ggtgtttcac
     4021 atcgtgcggc tcacgagtcg taccgacgcg cgcgagcgca cttacgaggc ggccgagcgc
     4081 acgattcgca tttcgatcgt gcaagagctc gtgaagcaac gtgaagcgag cttcgaaaag
     4141 gagctccgcg ccaagtaccc cgtcaacgtc gacgaagcgg cgctcgccaa agtccacgtg
     4201 ccgaagccga gccccagcgc aggcgccggc gtggtcttac ctactccgtt gcccggtcgc
     4261 gcgcggccgt gatcgagctc gcggtacccg gcgactggcg ttggcagctg cggcacgcgg
     4321 tgcgcagcgt cgccgagctc gagcgtcacc tttctctcac ccccgaagag ctcgaaggcg
     4381 cgcgccacgc cgaagatcaa ggctttcccc tcgcgatcac gccctattac ctgtctcttt
     4441 gcgatcgcga agatccgaat tgcccaatcc gccggcaggt cgtgccgctc gccgcggagc
     4501 agcgcagcgt cgccggtgac cttcgcgacc cgctcgggga ggaagcgcac gaggtcgctc
     4561 cagagctcgt gcagcgctac cccgatcgcg cgctgctctt ggtcagcgat cgctgcgccg
     4621 tctactgtcg tttctgcacg cgcagccgga tggtgggcga tggaggaggt ccgcgctcgg
     4681 acgcacggct cgcggctgcg ttcacgtatc tcgagaccca tcccgaggtg cgcgacgtca
     4741 tcgtgagcgg cggcgatccc ctcacgatgg cgaccgagcg cctgctgcgc atcatcgagc
     4801 ggctccgtac gatccccagc gtcgagacca ttcgcctcgc gacgcgcgtg ccggtcgcgt
     4861 tgccccagcg catcaccgac gagctcctcg gcgctctcaa gccgcaccat ccgctctggg
     4921 tgatgaccca cttcaatcac ccgaaagaac tcaccgaaga gtcgcgcgcg gcgctctgcc
     4981 gcctcgccga caacggcttt ccggtgatga accagacggt gctgctcagg ggcgtaaacg
     5041 acgatagccg cgtgctcacc gagctgttcc gcggcctcgt gaaggttcgc gcgcggccgt
     5101 actatctcct ccaggccgac ccggtgcgcg gcagcggaca ccttcgcacg ccgctcgcaa
     5161 cggggattcg gatcgccgaa gagcttcagg gcaggctctc cgggatcgct ctgccgaagt
     5221 tcatcgtcga cacgccgggc ggcttcggta aggtgccggt ggggcccgaa tgggtcgtgc
     5281 accgcgcgcc cggagttacg cgcttccgca cgccgcgcgg ggtcgaagtc gactacgtcg
     5341 atccgcccga atagcgagcg cgccggctac aaagggatgt tgccgtgctt gcgcggcggg
     5401 ttcttgtcgc gcttgttgcg gagcaagcgc aagtacgtgg cgagccgtgt gcgcacctcg
     5461 cgcgggcgaa tgacctcgtc gacgaagccg agctccgccg ccttgtaggg gttcgcgaag
     5521 cgcgcgcgat agtcttcgat gaactcgcgg cgagccgctt ccttgtcggg ggcgctctcg
     5581 atcgcgcttc gatagacgat gttgacggcg ccttccgggc ccatcaccgc gatttccgcg
     5641 gtggggaagg cgaggttcac gtccgcgcgg atgtgccggc tgctcatgac gtcgtaggcg
     5701 ccaccgtaag ccttgcgggt gatcacggtg agcttcggca cggtcgcctc cgcgaacgcg
     5761 tagaggagct tcgcgccgtg cacgatgatg ccacggtgct cttgctccac tcccggcaag
     5821 aagcctggca cgtcgaccca ggtcacgatc gggatgttga agcagtcaca gaaccgcacg
     5881 aagcgcgcgg ccttgatgct cgcgtcgatg tcgagcacac ccgcgagcac gagcggctgg
     5941 ttggcgacga ttcccactgg ctgaccgccg atgcgcgcga agccaacgat gatgtttcgc
     6001 gcgaattcgg cgtgaatctc gtaaaagcgc tcgtcgtcca cgaccttttt gatgagagcg
     6061 cccatgtcgt agggcttgtg gctctcttcc gggatgaggt tgtcgagctc tgggatttcg
     6121 cgctcgctcg gatcgttcgt ggcgtgtcgc ggcggatctt ccgtgttgtt ctgggggaga
     6181 taagagagca gctcgcgcac ctgggccagg cacgcgtagt cgtcgcgcgc ggtgacgtga
     6241 cagacgccgc tcttggccgc gtgtgccgat gcgccgccga gagcctcttt cgtgacttct
     6301 tcgtgcgtca ccgcgcggat cacgtcgggc ccggtgatga acatgtagct cgtgttctcc
     6361 accatgaaga tgaagtcggt gatcgcaggc gagtacaccg cgccgccggc gcacggcccc
     6421 atgatgcagc tgatttgagg gaccaccccg ctcgcgagcg tgttcttcaa gaataggtcg
     6481 gcgtagcccg cgaggctcgc gacgccttct tgaatgcgag cgccccccga gtcgcacagc
     6541 ccgaccacgg gggcgccgag ccgaagagcg tggtccatca gccgcacgat cttctcggcg
     6601 tgcgcggtgc cgagcgagcc gccgaacacg gtaaaatctt gagcgtagac gaagacaggg
     6661 cgcccctcga cggtgccgtg cccggtcacc acgccgtctc cggggatcgg cgcttgctcg
     6721 gccccgaact cgtggtggcg ctgcgcgacc aagcgtccga tttcgacgaa gctccccgga
     6781 tccagcaggg cgtcgatgcg ctctcgcgcg gtgagcttgc cggcggcgtg ctgcttcttg
     6841 acgcgctcag tgccgccagc gttgagggca cgcgcgtcgc gctcggcgag ggcatcgagg
     6901 gacggacgag acttgggctc gctcatgggc ggcggacctt accaccgagt cagggccgag
     6961 tgacgcggct catcgattcg cggcccgcgc ctcagtgcag agtcggcgac tccgctcgga
     7021 cgaacgagcg atagcagacg atggcgacga ttgcggccgc gatcgcccac atccaggagt
     7081 agccggtcgc gagcttgtcc cggtcgcacg cgtcgagcgc ccgaactcgc atttcggatt
     7141 cggcgagcgc tcgcgagacg gcggcgtcgg cgtcgcgtag cgggccgagc agagccggct
     7201 cgagcggaat accaccccgc gcggcgagca cggcgcgacc caaatcggcg agcgcgcgat
     7261 gctggcgctt ctcgaccgcg ccgaactcct tcttcttcga gtcgaggcgc tgctgaaagc
     7321 gagcgcggag gtcgcgtttt ttgcgctcga tttcgttcac gcgatagcgc acttcccgct
     7381 gatgcttggc gcggacggcg agctcgccga gcgtcgcttc gtacgccgcc ttcgccgctt
     7441 cgagctgcgg cacgagcgct tgctgctgcg cgcgcacgtg ttcggcttct tcgacgccgg
     7501 caccgcgctg ctccatcgcg cgcagctcga tgaagccgcg cttgtggcgc gcctcgagcc
     7561 gcagcaacgt ctgctcgcgc tcggcttgca ccgcgctgag ctcgccgagg gcgctttccg
     7621 cacttcgaag ctccggcaaa accgtcgaaa gctcggcgtc caggcgattg agctcggcgg
     7681 cttgctcctg cgcggccgcg ttttgaagct gcgcgcgagc gctgccctgc gcgtccattt
     7741 ccgcgacctc gtgcacgaag cgctggaacc cggggtttcc cgagagcgcc tcgcgcttct
     7801 gctcggcgag gcgcgccagc aagtcgtcgc gcgcgctttc gacacgggcg agctccgctt
     7861 cgcattggag aagctcgcgc cgcaagcgtc gccgctgcgc gaagactcgc agcgcgtaca
     7921 agacgttcag gacgccgagc gacgacggcg agccgtagcg agcgagcgcc tcgacctgct
     7981 tcgggtcgaa ctcgaggagc gctcgctcct cggtgacgcc gctcggccaa ttcgcgcggc
     8041 cttcgggcgc gaccgttatc cccgcgactt cgggcaacat cgagtcgagc tgaaaccctg
     8101 ctctgcccgt gacttcgtga gcggggatct ccgagcctcg cacgctcgag atcggcgagt
     8161 tcaaatccaa atccagcgga gggccggcgg gaagttcgga gagcccagtg accatcggcg
     8221 cgcgctggcg ctcaccgctt tcgctgaagc ggaccgcggc gagtctcgcc tgggacgcgc
     8281 tcggcttggg tggcggcaaa tcgagatcgg ggatctcgaa ccctggctcg ctcttgcgac
     8341 ccttcactgg gcccgtctcc gatctcgtcg ttgcacgatt ctttcagcgt atccgctcgg
     8401 agttcgagcg tcgaggcgac gcctttacat gtcgctggcc ctctgctacg acccctccgc
     8461 gattcgtttt acgggtccgc caacagcgca gcttgaaccc cgccaggccc ggaagggagc
     8521 aacggtagag aaaagcgggt gtggcgggcc ctccaatcgc accaagccgg gactacgcga
     8581 gtgacgtagc ggcgacatga ggccgcgcgc ggcccgctaa acgccgaaca tttcgatgac
     8641 ggcgccgttg tgggcgagcg gcgcgtcgag cgcgaagtgt gtcagcgtgt gggcgacgtc
     8701 ttcggcgctc atgcgcggtg ggaacccgct gccctcgagc atgcgcgtat cgacggaacc
     8761 agggagcacg gccgccgtca tcagcccgga gtcggtgagc tccgcggcca ggctcttcat
     8821 gaagccaacg atgccccact tgctggcgca gtacgcgctg tgccgcggcg taccgatgct
     8881 cgaagagatg ctcgcgacga agacgatgcg cccccggcgc ttttccagca tatggggcag
     8941 gagcgcgcgc gtgagcacga acggggcgct caagttcacg ttgagctggc gcatccattc
     9001 ggcgcgcgaa agctcgtcca cgcgcgcccg aacgacctcg cccgcgttgt ggatcacgac
     9061 cgcaggcgcg ccaaagcgtc ccgcgacgtc cgcacccacg cgctcgacgc tgtcgagatc
     9121 gttcaagtcg agcggaacga tgtccaccga cggcgctccc acggcgcggc acgcctcgcg
     9181 taacccgctt cggtcttcgg cgtcgcgcgt gatcgcgacc aagcgcaagc ctcgcttcga
     9241 aagcgacagc gcggtcgcgc gcccgatccc tcggctcgtt ccggtgacga ccgcgagcag
     9301 cggttcgctc actttgctgc ctcaccgagc gcgagcgaaa gctcgagcgc gccgtccggc
     9361 gtagcgaggc ttccgccgcg cgcgatcgtg acgagccgcg cgtcgggttg ctcggcgccg
     9421 cgcgtggcaa ggcacgtgtg gaggagcgag aggcgacaga aagcgccgcg cgctcgggca
     9481 tgagtcaaaa gggcctcgac caccgcgtcg ccgatcgcct cttgcagcgc gaggcgttga
     9541 ctacaggctt gtgcgagcct cgctacggtg ccgagcccga gcagtcgctc gccgggaagg
     9601 tatgcaacgg tggcgcgccc aaccgccggc agcaggtggt ggggacacac cgcgacgacg
     9661 gcgatgtctc gcaccacgac gaggccgctc gtcgccgcgg tcgcgggctc ggaaccttcg
     9721 cgcaagagct ccgcgacgtc gacgcgctcg ccgcgcaaga gctcgtgcgc gaatgcttcg
     9781 gcgactcggt cgggagtgcc gacgagctct gatctttctt cgtatccgag ggcgcgcagg
     9841 aagatggcta tgccttgcgc cgccgctttc aaatcgggcg ctcgcatcgc gctcctctcc
     9901 actccgcgtc ccaaaactct agacgccgag cactagttca gtagcccttt gcgcacgtcg
     9961 gcgcggagcg ggcggcgacg gaagtagccc ccgccgagct cgtggtagta gccgagcgtg
    10021 tcttcgccgt agcgccagca gagccacacc atgcgtccgt cgacgcgacc gtaaaaatcg
    10081 atgaggccta tttgggggtc tttgacgacg ccaccgagct cgcgcacctt gctccagcca
    10141 gcttcgtagc ggcctacgcg gcgcctcagg tcggccttca agcgccggac ctcggcgctg
    10201 tcgccttctc ggtgctcgag tgagttcggg gtttcacctt cgagccgcgt gagctccgcg
    10261 aggccttgtt cgatgttgct ctgttcgcgc agctgctgtg cgacgaagac gctcagcgtc
    10321 ggaatcagcg cgttcacttc ggagatggtg aacacgcgag cgctgtccat cgagagatcg
    10381 ccgttcaacg cttgagatcc caaagcacgg tcgctgccgg cgtcagaacg ccggcgtctt
    10441 tcatggttcg acattctccg cagctctccg ggaacaggtg gttgtcagta caagcgcgac
    10501 agcggcgggc aactgaccgc gcgctctcca attcaatagc aagccgttgc aggagtgcca
    10561 tgcgttcgtt gatgcgctcg gcgtatcgct cgagagcgcc gtcgatagcc gccgcagcgt
    10621 cggcgccgct cgtcgcttcg cgtttgatcg cgaagaggtg ctttatttcc ggaagcgaca
    10681 gccccgcggc gcgaagctcg gaggcgagct tgagcttctc gagctcactc tccggaaaca
    10741 accgatgacc gccctcggcg cgcgcggttg ggcacaaaag gccggcttct tcgtaaaagc
    10801 gcacggtgcg cagcgtgctg ttcgttctgc gcgccatttc accggtcgtg agcagcccac
    10861 gcgcgggttc gctctggcta tctgcttccc taacgtcaga caagcgcatg gagcttatga
    10921 agcagatccc gatcggtcaa ggcgaccgcg acgattccga ctccgtcgcg agaacgcacg
    10981 ccgccgccgg tccgaaatcg atgcttcacg cttgctacac taatgcgctc cgcgtgtcag
    11041 aggctcgccg caggcgtgtc gaaggttcgt tcacgcagct ctgcgatccg tcacgtcggc
    11101 gccgcggtta ggcaaaactt cgttttttcg cttgcgcccg aggcgccggg aaatatcgct
    11161 attttgcgcg tcgtgagttc tctcgggatt cgggatcacc ccgtaggcca aaagatgatt
    11221 ccaactttgg tcagaaaaaa gtccgcgatc aaaattgcga tggaaatcgc gacaaccgtg
    11281 cgagtagtgc tgttgcctac accggcggtg ccgccggtcg tcgtcattcc gaaatgacac
    11341 ccgaccagcg caatgattgc gccgaagaac ggtgtcttca gcattccgct gacgaagtcg
    11401 gcgagtgtga ccgcactcag cgcagtacgc aggaagaacg cggacggaat gtcgaattgc
    11461 acgtccgtga tcaacatcgc gcccgtgaag ccgagcacga gagcgaacgc gcccaacatc
    11521 ggcatcacga tcaccgacgc gacgaggcgc ggccacacga gcttcttcaa aggatcggcg
    11581 ccgagcgcgc gcacggcgtc gatctgctcc gtcaccgaca tcgcgccgac ctcggcggcc
    11641 atgcccgacc cgatgcgtcc acctacgatg acggcggtga gcgtcggcgc gagctctcgc
    11701 gcgaacgata acgcgatcac gcggcccgtg tactccatgc cgccgaactt acgcagaccg
    11761 aacgcgaact gcgtggccat gaccatgccg atgaacacgc tcgtgaccgt gacgatgccg
    11821 accgacgcga ccccgagcac ctcgagctga gtgagaatcg ctttccactc gaagggacga
    11881 cggaaccccg cgcgcagtgt gcgcaccgtc atctctgcga tcacgccgag gtgcgagaag
    11941 aaggcgattc ctgcgtccac gcggcgcgcg gcccacgtcg gtggaggcgg gggcgcgagg
    12001 ctcgtgcggc tcggtcgatc ggcgccgtcg ctgctgttcg gcgagctgat ggtcatgggc
    12061 gtcgctcctc gagccgcgat gctttcgaat gagcagggag gctcgcaggg aaggtcaggg
    12121 cgctcaggct catgtccgct ccactcaggt gctcgtcgag aagcccgcga cgaagtggtc
    12181 gaaatcctcg acgctcgcgg cgggggcgcc ttcggctccg atccacatga agtcgtagac
    12241 gcagccatcc ttcttcagca cgtacacgac gaagtgcttc ggcacgccgt cgaggtgggc
    12301 gtcgagctcg gtgcgcagcg cgccgcgccc gtcgatgacg agctccttct gacttccgac
    12361 gacgcgattc gtgaagttca agaacagatg atgggtgagg gccgcgagcg gaacgtcgtc
    12421 gccgtcgacg ccgcagcgcc cgttcaccgc gacggtggct ccgctcggat catcgcgaaa
    12481 cgagagcgcg gcgccttcga cctcgatgct gcgccaggtt tggggaactg gcccgacgcg
    12541 aaacgcgatt tcactgcttc ggtagacctt gccgtcgaag gtagggccgc cgcagcccgg
    12601 tgttgcggcg agagcccagg ccaggagacc gagcgcaagg aagcgagcct tcatgtttcg
    12661 cggcgacgtt agcacgcccg cttgcagtgg catgaaatgc tctcccgccc cgctgtgtca
    12721 gcgccgaacc gctcgctcgc gagcgcggaa tgtgggtgct agcctgacct cggtcatgct
    12781 gccgatcagc gatcagaacc cgacgcgaac tttcccgttc gtgaattacc tgctgatcgc
    12841 gctgaacgtg ctggtcttcg gctggcaata cctactgttc tcgaccggcg gtgaggcggc
    12901 cgtttccggc ctcgggctcg tgcccgcgcg cttcagtcaa aacccagtgg gcgagctcgg
    12961 caacctgttc gcgagcatgt tcatgcacgg gagcctcggt cacatcgccg gtaacatgct
    13021 gtacctctac atcttcgggg acaacgtcga agatgcgatg gggcacttcc gctacatcgt
    13081 attttacctg gcgtgcgggt tcatcgcggc gctggcgcag gtggtggtcg cgccgatgag
    13141 cccccttcca atggtgggag cttccggcgc catcgcgggt gcgctcggcg gatacatcct
    13201 tctttatccg cgcgcgcccg taacggtcct gaacccgata ctgccgctct ggctgctctt
    13261 cggtttgttc tttcagctcc cggcttggct cgccatcggc atctggttcg tgccgaacat
    13321 cctgaacgct ttggtcgaag agagcacggc gggcggaatc gcgttcttcg cgcacatcgg
    13381 cgggttcgtc gcgggcatgc tcttggtgcg tgtcgccacg cggggtcggc ggcgcatcga
    13441 tcggggccgt tggtcgggct ggcgcgcgcc gccgcgcagg cctacgcgcg gttcatggtc
    13501 ggcgaatacg tcgcgatcat ggtactgatc gcggcggttc aatgccgctc gtcctctaca
    13561 acacgctgac ccgcaacaac gaagagctca accccaaagc agggcgggtc aagatgtact
    13621 gttgtggccc gacgacgtac gacgtgccgc acgtcggcca cgcgcgcgcc gcgctcgtgc
    13681 cggacatctt ggtgcgaagg ctgcgcgatc tcggcctcga cgtgacgtac gtccgtaaca
    13741 tcaccgacgt agacgacaag attctcgcgc gcgccaagca aaacggcgaa caaccgctcg
    13801 cgctctctca ccggatggcg ctggtttacc aagagcagat ggcgatggtc ggctgtgagc
    13861 ctccgactca cgagccgcgg gtcagcgaga acatcgccga gatctgctcg ttgatcgaac
    13921 ggctcatcgc gaacgaggcg gcgtacgtcg tcgacatgcc gggcggcaag accgacgtct
    13981 acttcgcggt gcgcaaattc gagagctacg gaaagctgtc gcggcgtcgt atcgatgact
    14041 tggtggtcgg cgcgcgcgtg gccgaaggcg aaaataagcg cgacccgctg gatttcgcgc
    14101 tgtggaaagg gacggggccc gaggactggg gctgggacag tccgtggggc cgcgggcgcc
    14161 cgggctggca catcgagtgc tcggcgatga gcggtcgcta cctcggccat ggcttcgacg
    14221 tgcacaccgg tgggatggat ttgatcttcc ctcaccacga aaacgagatt gcccagtccg
    14281 agagcctgac cggcaagccc ttcgtgcgct actgggtgca caacggcatg ctgcagctgg
    14341 gcggcgagaa gatgtc
//
LOCUS       DASTKS010000104         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1131486, whole genome shotgun sequence.
ACCESSION   DASTKS010000104 DASTKS010000000
VERSION     DASTKS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131486"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2211
                     /locus_tag="VFQ35_02230"
     CDS             <1..2211
                     /locus_tag="VFQ35_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9929469.1"
                     /translation="EGIARIRGLNAGSYDLRAVRGGDVSDPVLGFELARGAQETLTLR
                     LAPGRMVTAWVTDGAADDARSVPNADVVLVEGGLSAFPLRGRSGADGRVTLGPISRAA
                     ATLAASAPDFVPGQLVAVPDVLDGPVRIPLLRGGTLRGEVVDARGFPIAGASIEVVGT
                     DRSGLPIAQSPESQNFRAAHFEWALGGPPGLIAVGELGVMPGPVPPIPPPGAFSNAAT
                     SGGNALAQLETPSPALLAPWVTSDHGEFVARPVMPGRVRALVRHPEFVEGSSPLVSLS
                     SGAEAFVKVVLLRGGSLEGRVIDERGFPVPGVEVEVVAERGTFERSTITASDGAFAFA
                     AVPENVSVSVARPEDRARIAARKRVRVAEGGRERIELVIPPPREPIQLRVVDDHDAPV
                     DLAEIHASSLDPQISLRSTVFTNASGTAELADALDVPLRIAVEAPRFARAEKTFDKAP
                     KQIQITLDPGVLVTGRVTAVRGRRSVQGAVVTLRVGSRRTSSMTDADGNYRFASVGVG
                     HAEISVSHPDFAGATASADIQRTTRLDRPFELPTIDLSEAGEVSGEVVDERGNAVEGA
                     RVAVGLSPAFLPTGALPEGVALTDSRGAFTLRGLAEGTVKLSAYAPTVGRGSVGGVTV
                     NAGRTTRGVTIRLTEKAEADAAAPATLAVTLAERGGGDALEISIVHVATGSEAERAGL
                     AVGDVVQRIDGERPRSLADARTRLSGREGSDLVLELSRGGAPLRLRVTREPIRN"
     gene            complement(2235..3023)
                     /locus_tag="VFQ35_02235"
     CDS             complement(2235..3023)
                     /locus_tag="VFQ35_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929470.1"
                     /translation="MRVVHCVVVGVLLAGFGCREKQPEHLAPTASALAPVAPPPAEQA
                     VRMAVSSESSKVSFLMQAPIEKISGDAPGSMSGELFVNPSELAKSTGLVKVDLDKLTL
                     YQEKRENEKAAFSERAKNETQNQHARTWLEVSSDTPEDVRSANRYVEYRITRLDAPSA
                     PSLAALSGPERKVTATAVGDFRLHGRKAEKSVKVELTFSYQGDKLGSVRVRTLAPLQI
                     GLEEFDVRPREAFGKLAQKTLSALGSKVAEVAPIEIDFSATPAS"
     gene            complement(3119..3259)
                     /locus_tag="VFQ35_02240"
     CDS             complement(3119..3259)
                     /locus_tag="VFQ35_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929471.1"
                     /translation="MEPGLVTLAKIVGTLLFGMGVIPILFLLSGGDMTEPQDRLDSEH
                     KG"
     gene            3376..3819
                     /locus_tag="VFQ35_02245"
     CDS             3376..3819
                     /locus_tag="VFQ35_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929472.1"
                     /translation="MQSSIPASGPQAASALRRLLLIEDDPMVSRSLERAIGALRPELE
                     IRTAGSAERALALLAESAFDAAITDLNMPGLSGLVWLEKLRRECPSLVCVTHSSQLES
                     FGEDEVRRLSHGTFEKPAHASAMLDAIDRLLLQRGVAAQAGLRSA"
     gene            complement(3795..>4196)
                     /locus_tag="VFQ35_02250"
     CDS             complement(3795..>4196)
                     /locus_tag="VFQ35_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9929473.1"
                     /translation="GLASGAFEHALGYMHERQQFGKAIADFQGMQFQYARVAMEIEAA
                     RLLVYNAARLKDSGLDFVKEAAMAKLFASEVAERAASQAIEFMGGVGFTRDYPVEKFY
                     RDAKIGKIYEGTSNMQLQTIAKLLQAERKPA"
BASE COUNT          646 a         1444 c         1432 g          674 t
ORIGIN      
        1 gagggcatcg cgcggattcg cggactcaac gccggcagct acgatttgcg ggccgttcgc
       61 ggcggggacg tctcggaccc cgtgctcgga ttcgagctcg cgcgaggcgc tcaggagaca
      121 ctcacgctgc gcctcgctcc cgggcgaatg gtgacggcgt gggttacgga tggtgcggcc
      181 gacgacgcgc gctcggtgcc caacgccgat gtggtgctgg tcgaaggagg actcagcgcc
      241 ttcccgcttc gtgggcgcag cggcgccgac gggcgcgtca cgctgggccc gatttcgcga
      301 gccgccgcca cgctcgcggc aagcgcgccc gatttcgtgc cgggacagct cgtcgcggtg
      361 cctgacgtgc tcgacggccc ggtgcgcatc ccgctgctac gcggtggaac gctgcgcggc
      421 gaagtcgtcg acgcgcgcgg gtttccgatc gccggcgcgt ccatcgaagt cgtcgggacg
      481 gatcgttcgg ggctgccaat cgcgcaaagc cccgagtctc agaacttccg cgccgctcac
      541 ttcgagtggg cgctcggcgg accacccgga ctcattgcgg tcggcgagct cggcgtgatg
      601 ccggggccgg tgccgccgat cccgccgccc ggcgccttct cgaacgcggc tacgtcaggc
      661 gggaacgcgc tggcgcagct cgaaacgccg tcgcccgcgc tgctcgcgcc ctgggtgacg
      721 agcgaccacg gcgagttcgt tgcgcgcccc gtgatgcccg ggcgggtgcg ggccctcgtg
      781 cgacaccccg agttcgtaga aggctcgagc ccgctcgtgt cgttgagctc gggcgcggag
      841 gctttcgtca aagtcgtgct gctgcgcggc ggcagcctcg aaggccgcgt catcgacgag
      901 cgcgggttcc cggtgcctgg ggtcgaagtc gaggtcgtcg ccgagcgcgg caccttcgag
      961 cgctcgacga tcaccgcgag cgacggcgcg ttcgctttcg cggccgtacc agagaacgtc
     1021 agcgtcagcg tggcgcgccc agaagaccgt gcgcgcatcg ccgcgcggaa gcgggtgcgc
     1081 gtcgcggaag gcggccgcga acgcatcgag ctcgtgattc cgcctccgcg cgagcccatt
     1141 cagctgcgcg tcgtcgacga tcacgacgcg ccggtcgacc tcgcggagat ccacgcgagc
     1201 tcactcgacc cgcaaatttc gctgcgcagc accgtgttca cgaacgcttc gggaacggcg
     1261 gagctcgccg acgcgctcga cgtgccgctg cgcatcgcgg tcgaggcgcc gcgcttcgcc
     1321 cgcgccgaaa agacgttcga caaagcgccg aagcaaatcc aaataacgct cgaccccggc
     1381 gtgctcgtca ccggcagggt aaccgcggtg cggggacgcc gcagcgtaca gggcgccgtc
     1441 gtcacgctgc gcgtcggatc gcgccgcacg agcagcatga ccgacgcgga cggaaactac
     1501 cgcttcgcga gcgtcggcgt gggtcacgcc gagatttcgg tgtctcaccc cgatttcgcg
     1561 ggtgccaccg cgagcgcgga catccagcgc acgacgcgac tcgatcgacc gttcgaactg
     1621 cccaccatcg atttgtcgga agccggagag gtgagcggcg aagtggtcga cgagcgaggc
     1681 aacgctgtcg aaggagcgcg cgtcgcggtc gggctctctc ccgcgttttt gccgaccggc
     1741 gcgctccccg aaggcgtcgc actcaccgat tcgcgcgggg ccttcacgtt gcgggggctc
     1801 gccgaaggga ccgtgaagct cagcgcgtac gcgcccaccg tcggacgcgg cagcgtcggg
     1861 ggcgtgacgg tgaacgcggg gcgcacgacg cgcggcgtga ccattcgact caccgaaaag
     1921 gccgaggcag acgcggcggc accggcgaca ctggccgtga ccctggccga gcgcggcggc
     1981 ggggacgccc tcgaaatctc catcgttcac gttgcgaccg gcagcgaggc ggagcgcgcc
     2041 ggcctcgccg tgggcgacgt cgtccagcgc atcgacggcg agcgcccgcg ttcgctggcc
     2101 gacgctcgaa cgcgcctctc gggccgtgaa ggcagcgatc tcgtgctgga gctatcgcgc
     2161 ggcggcgccc cgcttcggct ccgggtaacg cgcgaaccga tccggaattg aagcaaaccg
     2221 cgagttcgcg gcgactacga ggctggcgtc gccgaaaagt cgatctcgat cggcgcgact
     2281 tcggcgacct tgctgccgag cgcgctcagc gtcttttgag cgagcttgcc gaacgcttca
     2341 cgcggccgca cgtcgaactc ctcgagaccg atttgcagcg gcgcgagggt gcgcacgcgc
     2401 accgagccga gcttgtcgcc ctggtacgag aacgtaagct ctaccttcac gctcttttcg
     2461 gctttacgac cgtgcaatcg gaagtcgccc accgcggtcg cggtgacctt gcgctcgggc
     2521 ccactgagcg ccgcgaggct cggggccgac ggcgcgtcga gccgagtgat gcggtattcg
     2581 acgtagcgat tggcggagcg cacgtcctcg ggcgtgtcgc tcgaaacctc gagccaggtg
     2641 cgcgcgtgtt ggttctgcgt ctcgttcttg gcccgctcgc tgaacgccgc cttctcgttt
     2701 tcgcgcttct cttgatagag ggtcagcttg tcgaggtcga ccttcacgag cccggtggac
     2761 ttcgcgagct cgctcgggtt cacgaacagc tcgcccgaca tcgagccggg cgcgtcaccc
     2821 gagatcttct cgatcggcgc ctgcatcaga aacgagacct tgctcgattc ggagctcacc
     2881 gccatgcgca ccgcttgttc ggcgggaggc ggcgcaaccg gagccagcgc cgacgcggtg
     2941 ggagcgagat gctccggctg cttctcgcgg caaccaaagc cagccaacag gacgcccacg
     3001 accacacaat gaacgactcg catgttgatc cctcgcggct acacagccgc tcaccttcgc
     3061 cccctctacg gtgcgaggtc gctctggtta cacgcgcccg cggcggacgc gcgtcgggtc
     3121 agcccttgtg ctcggaatcc aagcgatctt ggggctcggt catatcgccg cccgacaaca
     3181 agaacagaat cgggatcacg cccatcccga aaagcagcgt tcccacgatt ttggcgagcg
     3241 taacgaggcc aggttccatg acgagcggga cgataccgag atcccgagcg ggtgcaaggc
     3301 gtcgcgtccc ttcaggttgg agtcgcgcaa ctctgtagca gaccggtcgg cctgggactt
     3361 gcacaacggc ccggcgtgca gtcatcgatt ccggcatccg gcccccaggc cgcctcggct
     3421 ctgcggcgtc tgctgctgat cgaggacgac ccgatggtca gccgcagcct cgagcgcgcc
     3481 atcggcgcgt tgcggcccga actcgagatt cgcaccgcgg gcagcgctga gcgcgctctc
     3541 gctctgcttg ccgagagcgc tttcgacgca gcgatcaccg acttgaacat gccggggctc
     3601 tccgggctcg tctggctcga gaagttgcgc cgcgaatgtc cgtccctcgt ctgcgtgacg
     3661 cattcgtctc agctcgagtc gttcggcgag gacgaagtga gacggctgtc tcacggcacg
     3721 ttcgagaagc cagcgcacgc gagcgcgatg ctagacgcga tcgatcggct cctgctgcag
     3781 cgcggcgtcg cggctcaggc gggcttgcgc tctgcctgaa gcagcttcgc gatggtttga
     3841 agctgcatgt tgctggtgcc ctcgtaaatc ttgccgatct tcgcgtcgcg gtagaacttc
     3901 tccaccgggt agtcgcgcgt gaagccgacg ccgcccatga actcgatggc ctgcgaagcc
     3961 gcgcgctccg cgacctcaga agcgaagagc ttcgccatcg ccgcctcctt gacgaagtcg
     4021 agcccggagt ccttcagtcg cgcggcgttg tagacgagca accgcgcggc ctcgatttcc
     4081 atggcgacgc gcgcgtactg gaactgcatg ccttgaaaat cggcgatcgc cttcccgaac
     4141 tgctgccgct cgtgcatgta accgagcgca tgttcgaagg cgccactggc gagccc
//
LOCUS       DASTKS010000105         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1577642, whole genome shotgun sequence.
ACCESSION   DASTKS010000105 DASTKS010000000
VERSION     DASTKS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577642"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFQ35_02255"
     CDS             <1..472
                     /locus_tag="VFQ35_02255"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HET9929474.1"
                     /translation="PPALRGAALGYLWSLRALDTLTAETLATSALRRAALPTTLGDFL
                     AGLFALAREEVVTAAESELLAALDDVLGEMTPEDFLIAVPALRMAHAYFPPRERERIA
                     QRVLALHGRANASARDFLKLSAGADTLAAARSLEARVDELERRFGLEPSEEKDD"
     gene            465..1595
                     /locus_tag="VFQ35_02260"
     CDS             465..1595
                     /locus_tag="VFQ35_02260"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9929475.1"
                     /translation="MTDAKGDDLLERWRLVLGAPAARTMPNLSQRGQAMDAALEWLYG
                     RDPELAERGVQRSGGSGESQLTTPDWVNEVHRLFPKETIERLERDAVERFRIDDIVTN
                     LEVLERIEPSEALLRAVLHTKHLMNPEVLAKARELVAKVVEDLMRRLANDVRVSFSGV
                     RDRRRPSLHKSAKNFDLRRTIQTNLKHYQPELKRISLEKPRFFSRTRRFTERWQIILV
                     VDQSGSMVGSVIHSAVTAACLWGLPGMRTHLVAFDTEVIDLTADVADPVDLLMKVQLG
                     GGTDIAKAIDYAASLIEAPRRAIVVLISDFYEGGDAHRLVRATAALCEQGSKVLGLAA
                     LDADANPVYDRDLAQKLVDVGASVGAMTPGALASWIAEQVHK"
     gene            1592..3628
                     /locus_tag="VFQ35_02265"
     CDS             1592..3628
                     /locus_tag="VFQ35_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929476.1"
                     /translation="MSRDDLRALTPDALATLSNRGLVKRAQKELDSGQAPTLEELADG
                     TVVGRFGDVVTKLPPRVALSDAPCTCGAAGICRHKLMVALAYARAPTPSADAKSSESE
                     SPTANSIDAWSPGTFNDEELARVLGKRAWERARAVLRQGYVAEVRRATTKDQAPSAVL
                     PTCSVRFLVPRDLSYAHSDGGERAAEAIALAVWAFRAADLKDARAKRLELEVRDESAP
                     SAVVADELSPALGLAQELLLEGIVHARASMASQFERVRQTLTGARMLWPALVLEDLEQ
                     ELSAYHQRSARYSPAGAAALISELFARARAVERPGALPAARILGTEIAAETPLDHLRL
                     SSLGARIRGDAGQRLAEVVLAHPDTATLLVMRKEYPLASGRPAMLPSELGRKPFAPRA
                     TLSAIATGEVVTASARRAANRLLVLGQGRVARTSVVPSNGDWSGLPNGLRVRDTTKLA
                     NELAELPPRLVRARVLADGVYVLEIAEVRGLSYAPGSQRLSAVLVPHEGPNVRLVRTY
                     DAAAPGALEELAHALSGNSGPPRFVSGSVRHTGSGLELEPIGVVTDRFHVPDLADPSD
                     RPQFPGVTAPDVSDSGHVDPIMHTLDNAASVLDDAAHHGLRRASPSYAQQLAQRADEL
                     RRIGFTRLASRFEVLKSALADSQASGDADSEAAVVERWADAAIRLRLSLERRSV"
BASE COUNT          688 a         1377 c         1294 g          672 t
ORIGIN      
        1 gccgccggcg ctgcgcggcg ccgcgctagg ctacttgtgg tcgctgcgtg cgctcgacac
       61 cctcaccgca gaaacgctgg caacttctgc gctgcgcagg gcggcgctgc ccacgactct
      121 gggcgacttc ctggctgggc tcttcgcgct cgctcgggaa gaagtcgtga ccgcggctga
      181 aagtgagctg ctcgccgcgc tcgacgatgt cttgggggag atgacgcccg aagacttttt
      241 gatcgcggtg ccggcgttgc gcatggcgca tgcttacttt ccaccgcgtg agcgcgaacg
      301 catcgcgcag agggtgctcg cgttgcacgg tcgagcgaac gctagcgccc gggactttct
      361 caagctgtcg gccggggccg acacgctcgc ggcagcgcgt tcgctcgaag cgcgggtgga
      421 cgagctcgag cgacgcttcg gcttggagcc tagcgaggag aaggatgact gacgcgaagg
      481 gcgacgacct tctcgagcgt tggcggctcg tgttgggtgc accggcggcg cgcacgatgc
      541 cgaacctatc ccagcgcggc caggccatgg atgccgcgct ggagtggctg tatggtcgcg
      601 atcccgagct cgccgagcgc ggggtccaac gctccggcgg cagcggtgag tcgcaactca
      661 cgacgcccga ctgggtgaac gaggtgcacc ggctgttccc caaagagacc atcgagcggc
      721 tcgagcgcga cgccgtcgag cgctttcgaa tcgacgatat cgtgaccaac ctcgaggtcc
      781 tagagcgcat cgagccgagc gaggcgctgc tgcgcgccgt tctccacacc aagcacctga
      841 tgaaccccga ggtgctggcg aaagcccgcg agctcgtggc caaggtcgtg gaggacttga
      901 tgcgccgctt ggccaatgac gtgcgcgttt ccttcagcgg cgtgcgtgac cgacgtcgcc
      961 catctcttca caaatcggcg aagaacttcg acctccgccg caccatccag accaacctga
     1021 aacactacca gcccgagctc aagcgcatca gcctggagaa acctcggttc ttctcacgca
     1081 cgcgacgttt caccgagcgt tggcagatca tcctggtcgt ggaccaaagc ggcagcatgg
     1141 ttggctccgt gattcattcg gcggtcaccg cggcttgctt gtgggggctg cccggtatgc
     1201 gcacccacct cgtggcgttc gacaccgagg tcatcgactt gaccgcggac gtcgcggacc
     1261 cagtcgactt gctgatgaag gtgcaactgg gaggcggcac cgacatcgcc aaggccatcg
     1321 actacgccgc ttccctgatc gaagccccgc ggcgcgcgat cgtcgtgctg atcagtgact
     1381 tctacgaggg gggagatgcg caccgcctcg tgcgcgcgac tgccgcgcta tgcgaacagg
     1441 gaagcaaagt gttggggctc gccgcgctcg atgccgacgc gaacccggtc tacgatcgcg
     1501 acctcgcgca aaagctcgtc gacgtgggag cgagcgtcgg cgccatgacg cccggagcac
     1561 tagcgagctg gatcgccgag caggtgcaca agtgagccgc gacgacctac gcgcgctcac
     1621 gccggacgcg ctcgcgacat tgtcgaatcg gggtttggtg aagcgcgctc aaaaagagct
     1681 cgactcgggc caagcgccga ctctcgaaga gctagccgac ggcaccgtcg tcggccgttt
     1741 cggcgacgtt gtgaccaagc tacccccgcg ggtggccctg agcgacgcgc cgtgcacctg
     1801 cggcgccgcc ggaatctgcc gacacaagct catggtcgcg ttggcgtacg cgcgcgcgcc
     1861 gactccctcc gccgacgcga aatcgagcga gagcgaatcg ccgaccgcca actcgatcga
     1921 cgcttggtcc ccgggtacct tcaacgacga agagctcgcc cgcgttttgg gaaaacgcgc
     1981 ctgggagcgc gctcgtgcgg tgctccgtca gggctacgtc gccgaagtgc gtcgcgcgac
     2041 gaccaaagac caagcgccct cggccgtact gccaacctgt agcgtgcgct tcctcgttcc
     2101 gcgtgatttg agctacgcgc actccgacgg cggtgagcgt gcggccgaag ccatcgcgct
     2161 cgcggtgtgg gcctttcggg ccgcggacct caaagacgct cgggccaagc gcctcgagct
     2221 cgaagtccgt gacgagtccg ctccgagcgc ggtcgtcgcg gacgagctct cccccgccct
     2281 tgggctcgcg caagagttgc tgctcgaagg catcgtccac gcgcgcgcct cgatggcgag
     2341 ccaattcgag cgtgttcgtc agaccctgac cggcgcccgc atgctctggc cggcactggt
     2401 gttggaggac ctcgaacagg agctttcggc gtaccaccaa cgcagcgccc gttacagccc
     2461 cgccggtgcc gccgcgctca tcagtgagct attcgcacgc gcacgcgcgg tagagcgacc
     2521 tggcgcgctt ccggccgccc gcattctcgg caccgaaatc gcggccgaaa ctcctctcga
     2581 tcacctgcgg ctctcttcac tcggcgcacg catacgcggt gacgcgggcc agcggctggc
     2641 ggaggtcgtg ctcgcccatc ccgacacggc caccctgctc gtcatgcgca aagagtatcc
     2701 gctcgcgagc ggacgaccgg cgatgctccc gagtgaactc ggtcgcaagc cctttgcgcc
     2761 gcgtgcgacc ctctccgcga tcgcaaccgg agaagtcgtg actgcgagcg ctcgtcgcgc
     2821 cgccaatcga ctgctcgtcc tcgggcaagg ccgagtggca cgaacgagcg tcgtcccctc
     2881 caacggcgat tggagcggct taccgaatgg cttgcgggtg cgcgacacga ccaagctcgc
     2941 gaacgagctc gccgaactcc cgccgcgcct cgtgcgcgca cgcgttttgg cggacggcgt
     3001 ctacgtcctc gagatcgccg aggtccgagg gctgagctac gcgcccggct cccagcggct
     3061 gagcgccgtg ctcgtgccgc acgaggggcc caacgtgcgc ctcgttcgca cgtacgacgc
     3121 cgccgctcct ggcgcgctcg aagagctcgc tcacgcgttg agcggcaatt ccggcccgcc
     3181 gcgcttcgtg agcggcagcg tgcgccacac gggctcgggg ctcgagctag aaccaattgg
     3241 cgtcgtgacg gatcgttttc acgtgccgga tctcgcggat cccagcgatc gaccccagtt
     3301 cccaggcgtt acggcaccgg acgtctcgga ctccggtcac gtggacccaa tcatgcacac
     3361 gctcgataac gccgcgagcg tcctcgatga cgcagctcac cacgggttgc gtcgagcgag
     3421 cccaagctac gcgcagcagc tagcgcaacg cgccgacgaa ctgcgtcgca ttggattcac
     3481 gcggctcgcg tcgcgcttcg aagtcctcaa gagcgccctc gccgacagcc aagcgagcgg
     3541 cgatgccgat tccgaggcgg cggtggtcga gcgttgggcg gacgcggcca tcaggctccg
     3601 attgtctcta gaacgccgat cagtttgact gcgcggttct agctttggtt tgtgggggcg
     3661 gcacgcgcag ccccctcttc cgaaatgaat tcggaagaga ctccccccac gcggtcactc
     3721 cgtgcggagc cgttttccgc taccttcggc gaattcctaa ctagaaagcg gccagagtta
     3781 gggccgcgcg aacgttccca cgttcatcct cggtgcggtt cgctcaacct ttcgacttca
     3841 gcgagcgacg catacaaaaa ccaaccaagt ctcgacaaac gacctccgcg acgcgctgct
     3901 atccagtcaa tattgcttgg tattttacca agcacagcat tctaacgaat accaacggta
     3961 tgctgaattt cactagcaca cgtagccgag aacgccctcg tgaggcatgc tcggttcatg
     4021 ggtgttcggg c
//
LOCUS       DASTKS010000106         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1800457, whole genome shotgun sequence.
ACCESSION   DASTKS010000106 DASTKS010000000
VERSION     DASTKS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800457"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..355
                     /locus_tag="VFQ35_02270"
     CDS             182..355
                     /locus_tag="VFQ35_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929477.1"
                     /translation="MRIGRPKKGTKAVGTTAKSLRLANDQWAALKLLAEERHMTLHAI
                     MREAVLSVLRKSA"
     gene            461..1468
                     /locus_tag="VFQ35_02275"
     CDS             461..1468
                     /locus_tag="VFQ35_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929478.1"
                     /translation="MANREKKSSRTARPPAPARKRTRRASPDHVRGCSEEEANELVRR
                     LNEKLVLERVPGRVNRLRSALRSTDHINVEALGRHESWSVPLHSCFVSLSHGSLNSKT
                     TALLKMRAPARCRMEDVRAEVAELEPSLVYEGASQLLLSDRRPRYPRDKKQWDVRDSF
                     ELAQDLANLPDINMLGRAPVVSLFSRIGKECAWLDRMSKPDGRRPALRLQTAIARDLC
                     TVKKDAFNDDVEIDSERFSLDEAVEIVRLPKDATNRAFRASLNKRLERFAESKALEKA
                     ALRTKLMKLDERAAKSGRDSLVVKIQERLELRERRARQEREAPQSNEAAHGDADRRSR
                     N"
     gene            1795..>2036
                     /locus_tag="VFQ35_02280"
     CDS             1795..>2036
                     /locus_tag="VFQ35_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929479.1"
                     /translation="MIESGTAQGIDPIETRLTLAELDQLVLARLAAATSMPEAYAALR
                     EGAVHAARELHGVDVSGAAELRTAYRALAATLERVQR"
BASE COUNT          413 a          649 c          660 g          314 t
ORIGIN      
        1 aaccaccgtc tcctcgtggt cgtcttcgcc gaggtgatca atgacaaaga aatccgcatc
       61 atcagcgcgc gcaaagcgaa ccgcgcggac cgcaagaagt acgagcaaga gcgcgaaacc
      121 tgagcggcgc gtatttcgcg gccccggcgc gctgcaacgc gcgctctccg cggcgcgaga
      181 gatgcgcata gggcgcccca agaagggcac caaggccgtc ggcactactg ccaagtcgct
      241 gcgtctggca aacgaccagt gggccgcgct caagctcctg gccgaggaac gccatatgac
      301 gctgcacgcg atcatgcgcg aagcggtctt gagcgtgctg cggaaaagcg catagcgcgc
      361 agcggaacgg cagaggccga agcagaggcc ctcgcgtggg cgtgtgcaaa ggcgaacgcc
      421 gcggagggta gcccgcggcg ttcaggaggt gggttgggag atggccaacc gcgagaagaa
      481 gagtagcagg actgcgagac cgccggcccc cgcgcggaag cggacgcgtc gtgcgagccc
      541 cgatcacgta cgtggatgca gcgaagagga ggcgaacgag ctcgtgagac ggctcaacga
      601 aaagctcgtt ctcgagcgcg tcccggggcg tgtgaacagg ctacggtccg cgctcaggtc
      661 aacggaccac atcaacgtcg aggctctcgg gcgccacgaa tcttggagcg tgcccctaca
      721 ttcgtgcttc gtttcgcttt ctcatggttc actcaattcg aagaccacag cgctactcaa
      781 gatgagagcc cccgcgcgtt gtcgtatgga agacgttcgg gccgaggttg ccgaactcga
      841 gccctcgttg gtctatgagg gggcttcgca gctcctgctc tcggatcgtc ggccgagata
      901 cccgcgagac aagaaacaat gggacgtgcg cgacagcttt gagctggcgc aagacctcgc
      961 gaaccttcca gacatcaaca tgctcggtcg tgcgccggtg gtttcactat tctcccggat
     1021 tgggaaagag tgcgcgtggc ttgacaggat gtccaagccc gacggcagga ggcccgcgct
     1081 acgactgcag accgccatcg cgcgcgacct ctgcacggtg aagaaggacg cgttcaatga
     1141 cgacgtcgag atcgactccg agaggttctc ccttgatgag gctgtcgaga tcgtccgcct
     1201 tcccaaggac gcaacaaacc gcgccttccg ggcgagtcta aacaaacgac tcgagcgatt
     1261 tgccgaaagc aaggcgctgg aaaaggcagc gctccgcacg aagctcatga agctcgacga
     1321 gagagcggcg aaatcagggc gcgactcgct tgtggtgaaa atccaagagc gcctcgagct
     1381 gcgcgagcgc cgcgcacgac aagagcgcga ggcaccacag agcaacgagg ccgctcacgg
     1441 tgacgccgat cgacgttccc gaaactgatc caagtaatcg cttttgatta caggcgcttg
     1501 ggcgagcccc gcagttgggt cctatgcggt cgtagaccgc cccaagatgc cacatcaaat
     1561 ccaagcgtcc gcacagcatg actctgcggc cgaaatcgcc cgcacggtcg ttgcggactt
     1621 gcctccgctc gtaccgttcg ccgtcgccgc caaggtcctg catcgttcgc cccgccagct
     1681 ccatcgagac cgccgtgcaa agcggtttcg ctcggttcaa ttcgcggacg gcggatcgag
     1741 ccgaatcctg attccgcgtt gcgaagtgca gcgcctcatc caggaggctc tctcgtgatc
     1801 gagtcaggca cggcgcaagg tatcgatccg atcgagacgc gcctcacact cgccgagctc
     1861 gatcagctcg tgctcgcgcg gctcgccgcg gccaccagca tgcccgaggc ctacgcggcg
     1921 ctgcgggagg gtgccgtcca tgcggcgcgc gagctgcacg gcgtcgacgt gtcgggtgcg
     1981 gccgagctgc gcacggcgta ccgagcgctc gccgcgacgc tcgagcgcgt gcagcg
//
LOCUS       DASTKS010000107         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_17337,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000107 DASTKS010000000
VERSION     DASTKS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17337"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFQ35_02285"
     CDS             <1..609
                     /locus_tag="VFQ35_02285"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9929480.1"
                     /translation="LALGLALVAVAFIDLEFMLVPFSLTLGATALGLATAGLRGVDFV
                     HVLWGAAFGFLLILVPFYWLYGLWRGYPGMGFGDALLLLVAGAWFGWEGVVFAFVAGC
                     AQATVVTLAAYFSGGHIELPDAVLEERKKLEEEIQAATGEERAELERERDKDTVLVEL
                     PRTFGKMRIPLGPFLALGILEYALFERVGLGALLDGIFHPEL"
     gene            606..989
                     /locus_tag="VFQ35_02290"
     CDS             606..989
                     /locus_tag="VFQ35_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929481.1"
                     /translation="MRVSLLPLALVLACARRPEPPRPVGNEFYEVEYPPPPAQIEIRD
                     EELAGRPECRFVDGHYEWRARHWQWQPGSWAVPPADCYYAPPVVEWGAPPNARLYYTP
                     PRWYRADGKSCQAPAPCLSRPQPSG"
     gene            986..1867
                     /gene="prmC"
                     /locus_tag="VFQ35_02295"
     CDS             986..1867
                     /gene="prmC"
                     /locus_tag="VFQ35_02295"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9929482.1"
                     /translation="MSTTDDASWSIGRVIRWAAEDFARKGFASPRLDAELLLGYVLGV
                     DRIRLIVDAGKPLEERELTAYRELIKRRRRGEPIAYIRGEREFYGLTFKVDKRVLVPR
                     PDTETLVETALGRTKERSLYGRALDLCTGSGCVAIAFARTRPTWRVTASDISPEALSV
                     ARENSERLGSVFNTSFVQGDLFEALPNDARFELVTGNPPYIARPEIAELQVDVRDFEP
                     RLALDGGEDGLDLVRRIVDQAPNFLEPQGVLALEIGFDQASAVAELFAARGFRDVVRD
                     RDYAGIERVVSGVYGPP"
     gene            complement(1852..2481)
                     /locus_tag="VFQ35_02300"
     CDS             complement(1852..2481)
                     /locus_tag="VFQ35_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929483.1"
                     /translation="MKRFAFLLGAAALLSPSLALAENEGWDGGYGIKAERRSGFVAAV
                     GLGMGLGVANGYPSALSKQGNPLYESSIDGGLGSSWSLWAGGALRDWFIFGLGTTSFS
                     AASGDLKARGGAFILHIETFPAWSLGGRFRDLGVFTEFGAGGMTIEGGPEKADGGLVS
                     VLGLGANYELFRWGHIELGPVISSNYMYSDSMKAFGVFAGVRSTFYGGP"
     gene            complement(2549..2872)
                     /locus_tag="VFQ35_02305"
     CDS             complement(2549..2872)
                     /locus_tag="VFQ35_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9929484.1"
                     /translation="MSFGREVRRRRGALGLTLEQLAERSGLTPNYIGTVENGRRDPSL
                     STVLALAKGLGVAPSALMGGVGEFSAEATEAAALVESLPAEVQVAMLELLRALGRRRR
                     GEPTR"
     gene            3166..4500
                     /locus_tag="VFQ35_02310"
     CDS             3166..4500
                     /locus_tag="VFQ35_02310"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9929485.1"
                     /translation="MARRLSGLSRSIELALMLAMVSACGSRGSSLSKVEGDGAIPDVT
                     PPPANGPRLGAIANATPVLERPTANARWIGALHAGALVPRSENALRKSKECEAGYYPV
                     FPRGVVCLNQGATLDLSHPTLAAMAIQPALEQPLPYTYGRVRKDGALYERDPARPDGV
                     RETQKLPRGSAMAVVGSWTARIGDGETERFGLLTNGRFVRASELEAARGSSFSGYAID
                     AEHELPVAFVVKRGVSNFKFDGDRIAKDEPLEYHQIIPLGGKNKNYNQSKIWAVDDEH
                     WLREQDVTIVHRRLRYPDFAKDGVRWLDVSVICNTLVAYEGKKPMFATLVSTGRDRVL
                     DNPPPDAAVTKLGTFEIANKHVTWLGAPPERAGERYSLFDLPWAIELTSGQALLGAYW
                     HDRFGVERGPGDIALSPADARWVFDFVGPELPKSWHEAAADPNQKTFVVVHK"
     gene            complement(4517..5530)
                     /locus_tag="VFQ35_02315"
     CDS             complement(4517..5530)
                     /locus_tag="VFQ35_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9929486.1"
                     /translation="MLVSPDAENPASAASFPMPGSIPPPSSTPRILGPLQALSPAMQR
                     VLAVLERLAPTELTVTLIGETGTGKDVLAHALHDHSGRAQAPFVVFDCGAVAANLAES
                     ELFGHERGAFTGAHSAHAGAFERADGGTLFLDEVGELALDLQPRLLRALENRRVRRVG
                     GNQYRSVDLRVVAATNRDLKSLVAAGRFREDLYFRLAAAVVAVPALRERTEDIPLLVR
                     RLLEEAGSPGIAVSEACYASLRAHAWPGNVRELKNALACALAFVDAAGVLEPQHLQLL
                     TPPPDSSSVERLPLGGQPLARIERAAIKQTLVYTRGNKVLAAQVLGIAVSTLYEKLKK
                     YGL"
BASE COUNT         1107 a         2048 c         1942 g         1032 t
ORIGIN      
        1 ctcgcgctcg ggctcgcgct cgtggcggtc gcgttcatcg acctcgagtt catgctggtg
       61 cctttctctc tcacgctcgg tgcgacggcg ctggggctcg ccacggcggg tctacgcggc
      121 gtcgacttcg tgcacgtgct ctggggcgcg gcgttcggct tccttttgat cttggtgccg
      181 ttctattggc tctacggttt gtggcgtggc tatccgggca tgggcttcgg cgacgctttg
      241 ctcttgctcg tcgcgggcgc gtggttcggg tgggaaggcg tcgtcttcgc gttcgtcgcc
      301 ggctgcgctc aagcgacggt cgtgactctc gccgcctact tttcgggagg gcacatcgag
      361 ctcccggatg ccgtgctcga agagaggaag aagctcgagg aggaaatcca agcggccacg
      421 ggtgaagagc gcgcagagct cgaacgcgag cgcgacaaag acaccgtgct cgtcgagctg
      481 ccacgcacct tcgggaaaat gcgcatcccg ctcggcccct ttttggcgct cggcatcctc
      541 gagtacgcgc tcttcgaaag ggtcgggctc ggcgcgctcc tcgacggaat ctttcacccc
      601 gagctatgag agtctcgctc ctacctctcg ccctcgtgct cgcttgcgcg cgcaggcccg
      661 agccgccgcg cccggtcggc aacgagtttt acgaggtcga gtaccctcct cccccagctc
      721 agatcgagat tcgcgacgaa gagctcgcgg gaaggccaga gtgccgcttc gtcgacggcc
      781 actacgaatg gcgcgcccgg cactggcagt ggcagcccgg cagctgggcg gtgcctcccg
      841 ccgactgcta ttacgcccct cccgtcgtgg aatggggagc gccacccaat gcgcggcttt
      901 actacacgcc cccccgctgg taccgagccg acggaaagtc gtgtcaagct cccgcacctt
      961 gcctgagccg tccccaaccg agcggatgag caccaccgac gacgcctcgt ggtcgatcgg
     1021 tcgcgtgatt cgctgggcgg ccgaagattt cgcgcgcaag ggtttcgcga gtccgcggct
     1081 cgatgcggag ctcttgctcg gctacgtgct cggggtcgat cgcattcggc tgatcgtcga
     1141 cgccggaaaa ccgctcgaag agcgcgagct caccgcctac cgcgagctca tcaagcgccg
     1201 gcggcggggc gagcccatcg cgtacattcg gggagagcgc gagttttacg ggctgacttt
     1261 caaggtcgac aaacgcgtgc tggtgcctcg gcccgacacc gaaacgctgg tcgagactgc
     1321 cctcggacga accaaggagc gctcactcta cggtcgtgcg ctcgaccttt gcactggctc
     1381 cggctgcgta gcgatagctt tcgcgcggac gcgcccgacg tggcgcgtga ccgcgagcga
     1441 catttctccc gaagcgttga gcgtggcgcg cgagaattca gagcgcctcg gctccgtctt
     1501 caacacgagc ttcgttcagg gcgacttgtt cgaagcgctc ccgaacgacg cgcgcttcga
     1561 gctcgtcacg ggcaacccgc cttacatcgc gcgccccgag atcgcggagc tccaagtcga
     1621 cgtgcgtgat ttcgagccgc gccttgcgct cgacgggggc gaggacgggc tcgatttggt
     1681 gcgccgcatc gtcgaccaag cgccgaactt tctcgagcca caaggggtac tcgcgctcga
     1741 aattggcttc gaccaagcga gcgcggtggc cgagctgttc gccgcgcgcg gctttcgcga
     1801 cgtcgttcgg gaccgggact acgccggcat cgagcgcgtg gtgagcggcg tctacggccc
     1861 gccgtaaaag gtcgatcgca ccccggcgaa gacgccgaac gccttcatcg aatccgagta
     1921 catgtagttg ctcgagatga cgggcccgag ttcgatgtgg ccccagcgaa acagctcgta
     1981 gttggcgccg agcccgagca cgctcacgag accgccgtcc gccttctcgg ggccgccctc
     2041 gatggtcatg ccgcctgcac cgaactcggt gaaaacgccg aggtcacgaa aacgcccgcc
     2101 gagtgaccaa gctggaaatg tctcgatgtg caagatgaag gcgccgccac gcgcttttag
     2161 atctcccgaa gcggccgaga agctcgtggt gccgaggccg aagatgaacc aatcgcgcag
     2221 cgcgccaccg gcccacagcg accatgagga accgagcccg ccgtcgatcg acgactcgta
     2281 caaagggttg ccctgcttcg agagggcgct cggatagcca ttggcgacgc cgaggcccat
     2341 gccgaggccc accgccgcga cgaagccaga acggcgctcg gctttgatcc cgtagccgcc
     2401 atcccaccct tcattttcgg cgagagccag gctcgggctg aggagggcgg ccgccccgag
     2461 caggaaggca aagcgcttca tggcccctgt tttcgggtca agcccggagc cggtcaagct
     2521 cgaaggcgca gcggcccaaa aaccgaggtt agcgagtggg ctcgccgcga cggcgacggc
     2581 cgagcgcgcg cagcagctcg agcatcgcga cctgcacctc ggccgggagc gactccacga
     2641 gcgcggccgc ctcggtggcc tcggcgctga actccccgac gccgcccatc aacgcgctcg
     2701 gtgcaacgcc gagtcccttc gccaacgcga gcacggtgct gagcgacgga tcgcgtcgac
     2761 cattctccac cgtgccgatg tagttcggcg tcaaacccga tcgctctgca agctgctcga
     2821 gagtcagacc caacgcgcca cggcggcgcc gaacctcgcg tccgaacgac actggaacca
     2881 gacttcttct cgttcctgag cctgacatga accactgata gtgttgctac tcaacacgat
     2941 cagataccga aaacgcgatc ggctcgccag tcgcaaggcc gggccgcgct gcagtccggt
     3001 aaagcgcggc attcttggac tgggcggccc gagaaaatga ctactcagat tttctgagcg
     3061 cgagctcggt tcgaccccgc ccccacactc tccggcatgg cgcccgtgta cgagtatcgt
     3121 ccacaaattt ggccgttcgc cctgatgtct gagagggaga gcgtcatggc tcgccgcctc
     3181 tccggtctca gcagaagcat cgagctcgca ttgatgctcg cgatggtgag cgcatgcggg
     3241 tcccgcggct cgtccctcag caaagtggaa ggtgacggcg caattccgga cgttacgccg
     3301 ccgcctgcga acgggccgag actcggcgcc atcgccaacg cgaccccggt gctcgaacgt
     3361 ccaactgcca acgcgcgctg gatcggcgcc ttgcacgcgg gcgcgctcgt cccgcgttcc
     3421 gagaacgccc tgcgcaagag caaggagtgc gaggcgggct actaccccgt ctttccgcgg
     3481 ggcgtcgtgt gcctcaatca aggcgcgacc ctcgacctct cccacccgac cttggccgcg
     3541 atggcgattc agccggcgct cgaacagcca ttaccgtaca cgtacggccg agtgaggaaa
     3601 gatggggcgc tctacgagcg cgatccagct cgcccggacg gggtccgaga gacacaaaaa
     3661 ttgccgcgcg gttccgccat ggcggtcgtg ggctcatgga cggcgcggat cggcgacggc
     3721 gaaacggagc gcttcggcct cctcaccaac gggcgcttcg ttcgcgcgag cgagctcgag
     3781 gccgcgcgcg ggtcgagctt ctcgggttac gcgatcgacg ccgagcacga gctgccggtc
     3841 gcgttcgtcg tcaagcgcgg cgtatcgaac ttcaagttcg acggagatcg catcgcaaag
     3901 gacgagccgc tcgagtacca ccagatcatc ccgctcggcg gaaagaacaa gaactacaac
     3961 cagagcaaga tctgggcagt cgacgacgag cactggctcc gcgagcagga cgtgaccatc
     4021 gtgcaccgac gcctgcgcta tcccgacttc gcgaaagacg gcgttcgctg gctcgacgtc
     4081 agcgtcatct gcaacacgct cgtcgcgtac gaaggcaaga agcccatgtt cgcgacgctc
     4141 gtgagtacgg gacgcgatcg ggtactcgac aatccgccgc ccgacgccgc cgtcacgaag
     4201 ctcgggacct tcgagatcgc gaacaaacac gtcacttggc tcggcgctcc gccggagcgc
     4261 gcgggcgagc gctacagcct gttcgacttg ccctgggcca tcgagctcac ctccggacaa
     4321 gcgctcctcg gagcgtactg gcacgatcgc ttcggcgtgg agcgcggccc gggcgatatc
     4381 gcgctctctc ccgcggacgc gcgctgggta ttcgacttcg tgggcccgga gctaccgaag
     4441 tcgtggcacg aagccgcggc cgacccgaac cagaagacgt tcgtggtcgt tcacaagtag
     4501 acgagcgaga cgcgattcag agcccgtact tcttcaactt ctcgtacaac gtcgacaccg
     4561 caatacccaa gacttgggcc gcgaggacct tgttgccgcg ggtatagacc agagtctgct
     4621 tgatcgctgc acgttcgatt cgcgccagag gctgacctcc gagcggtagg cgctcgacgc
     4681 tggacgagtc tggtggtggg gtgagaagct gtagatgctg tggttcgagc acgccggccg
     4741 cgtcgacgaa cgccagggca cacgcgagcg cgttcttgag ctctcgcacg ttgcctggcc
     4801 aagcatgcgc tcggagggaa gcgtaacagg cctcactcac cgcaataccc gggcttccag
     4861 cctcttcgag cagacgcctc acgagtagcg gaatgtcttc cgttcgctct cgcaaggccg
     4921 gaaccgcgac aacggcggcg gcgagacgaa agtatagatc ttcgcgaaag cgaccggcgg
     4981 cgacgagcga cttcagatcg cgattcgtcg cggccacaac acgcaagtcc accgagcgat
     5041 actggttgcc accaacccga cgcactcgac ggttctcgag cgcccgcagt agccgcggct
     5101 gcagatcgag tgcgagctcc ccgacctcgt cgagaaacaa cgtcccacca tccgcgcgct
     5161 cgaacgcgcc ggcgtgtgcc gaatgcgcac ccgtaaacgc gccgcgctcg tgaccgaaca
     5221 actcgctttc ggccaagttg gcggcaactg ctccgcaatc gaatacgacg aacggtgctt
     5281 gggctcgacc gctgtgatcg tgcagcgcgt gggccaagac gtccttgccg gtacccgtct
     5341 cgccaatcag cgtcacggtg agctcggtcg gagcgaggcg ctcgaggacg gccagcacgc
     5401 gctgcatcgc aggcgagagc gcctgcaagg gccctaaaat ccgcggcgtg ctcgagggtg
     5461 gagggatcga cccgggcatc gggaagctcg ccgctgaagc gggattttct gcatcggggg
     5521 aaaccaacat tcgttaccgt cggctgctcg gtgaggaccg gtgagccgca aactaccgcc
     5581 tcatccacga aaatgagttc cgcgcgctcc acggcgcagg atcatcgctg cacacgcaat
     5641 aacgagtcag cttgtagcag cagtcgtgta ttgaacggtg ttacattttg gcctccacgc
     5701 ttgccgcgaa cccgcggaag atctggctca ttttcctcta atcagagctc gaatgtgacg
     5761 tgcctttgac gggaatcgcg agaccgtcgc tgaaacgcca tcggtcgagg acttcgaaca
     5821 aatcggggcg cggacgcaac tccctccgcc cccgtgttgc cgcagcgttc gaccccgccc
     5881 ccccggctac gagcccatcg ctccggcagc ctcgcttgct ccacacgcgc accgccgacc
     5941 aagtgcagtt ctttttaagg ctgtgtgtcg gattttttgg cgaaacatca ttgcaattga
     6001 cgaaaaatcg gttgagctgc ggccgctgct gcgacgcggc ggagcacgaa aaactcggtc
     6061 aaacgctgca ctgattcgac tggcgcggaa gctgctttga ggcggggctt cctcgatgct
     6121 cgaaaagct
//
LOCUS       DASTKS010000108         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_17748,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000108 DASTKS010000000
VERSION     DASTKS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17748"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFQ35_02320"
     CDS             <1..349
                     /locus_tag="VFQ35_02320"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HET9929487.1"
                     /translation="FASSRLDWTERATEDSQRTLTLYTQALELRADDPVLSGSGREGL
                     SARAMGDVLCVERRLETERRVLLVNFGRRDCALSELGLNLGETRPLLRSDPTPHDDVL
                     PAEGALLLAATAS"
     gene            361..>2995
                     /gene="treS"
                     /locus_tag="VFQ35_02325"
     CDS             361..>2995
                     /gene="treS"
                     /locus_tag="VFQ35_02325"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET9929488.1"
                     /translation="MSEADWYKDAIIYELRVRSFYDSDGDGIGDLQGLTQKLDYLRDL
                     GVSAIWLLPLYPSPLKDDGYDIADYHDVHSAVGTLDDFKAFLEQAHARGLKVITELVL
                     NHTSDQHPWFERARRAPAGSRERNFYVWSDDPHKWSEARIIFQDFETSNWTWDPVAKA
                     YYWHRFFSHQPDLNFESEDVRRAMFEVVDFWFEMGVDGMRLDAVPYLYEREGTTCENL
                     PETHAFLRMLRAHIDAKFQNRMLLAEANQWPEDAVAYFGKGDECHTAFHFPVMPRLFM
                     ALRMEDSYPIIDILEQTPPIPDNCQWALFLRNHDELTLEMVTDEERDYMIARYAADRQ
                     TRINLGIRRRLAPLLDNDRRRIELMNMLLLSLPGTPVIYYGDEIGMGDNAYLGDRNGV
                     RTPMQWSPDRNGGFSRANPQRLILPTIIDPEYSYETVNVEVQQGNPSSLLWWMKRLLA
                     LRKKSLAFGRGSLQMLAPENRKVLAFLRRHHTDVILVVMNLSRFPQWVELDLSEFKSQ
                     SPVELFGNGAFPPIDGNPYRLTLGAYDGLWLSLTSATSSGGSQHPSLVPRRHLAFNGD
                     YAQLLKQESAALDIALTAYIPSQRWFRSKTLRVERARLREALPLGPRLFLAFIEVTFA
                     EGAPELYVLPLGIVEQTKEPDLPLVSIIKDADSPPHAWVYDASNDADTALALQSLALG
                     GRQLETRFVSIEGRRVVSGEAPEVPSAKALRAEQSNTSYLYADRYVGKLVRKVEIGES
                     PEVELLGALQRGPKGASVPPLVAHIQAELAGGVATLMTLLGYVPNEGNAWDLAVDHAQ
                     HFYEQVLAKHRDEPPPGTVSLFDEATNPTAISSDFLALAKLLGTRTAELHSALLANLS
                     GTPLGPRPYTTLTSRAFYQSVR"
BASE COUNT          566 a         1011 c          889 g          529 t
ORIGIN      
        1 cttcgcgtcg tctcggctcg attggaccga acgcgcaacc gaggattcgc agcgaacgct
       61 cacgctctac acccaggcgc tcgagctgcg ggccgacgat ccagtactca gcggttcggg
      121 ccgcgagggg ctctccgcga gggcgatggg cgatgttctg tgcgtggagc ggcgcctcga
      181 gaccgagcgt cgcgtgctcc tcgtgaactt cggccgacgc gattgcgcgc tttcggagct
      241 cggactgaat ctcggcgaaa cgcgcccctt gctacgaagc gaccctacgc cccatgatga
      301 cgtgttgccg gcagagggcg cgctgctgct cgcggcaacg gctagctgac acgaggacca
      361 atgagcgaag ctgattggta caaggatgcg atcatttacg agctgcgcgt gcgctcgttc
      421 tacgactccg acggcgacgg catcggagac ttacagggcc tgacccagaa gctcgactac
      481 ttgcgagatc tgggtgtatc ggcgatttgg ctcttgccgc tgtatccgtc gccactcaaa
      541 gacgacggct acgacatcgc ggattaccac gacgtccact ctgcggtcgg caccttggac
      601 gacttcaagg cgttcctcga gcaggcgcac gcgcgcggcc tcaaggtgat cacggagctc
      661 gtgctgaacc atacgtcgga tcagcacccc tggttcgagc gagctcggcg agcccccgcc
      721 gggagccgcg agcgcaactt ctacgtttgg agcgacgacc ctcacaagtg gtcggaagcg
      781 cgaatcatct ttcaggattt cgagacctcc aactggacct gggacccggt tgcgaaggcc
      841 tactactggc accgcttctt ttcgcaccag ccagacctga acttcgagag cgaagacgtc
      901 cgccgcgcca tgttcgaggt cgtcgacttc tggttcgaga tgggcgtcga tggaatgcgc
      961 ctcgacgccg ttccttacct gtacgagcgg gagggaacga cctgcgaaaa cctgcccgag
     1021 acgcacgcct ttttacggat gttgcgcgcg cacatcgacg cgaaattcca aaaccgaatg
     1081 ctgctcgcgg aggcgaacca gtggcccgag gacgcggtcg cgtacttcgg caagggcgat
     1141 gagtgccaca ccgccttcca cttcccggtg atgccgcgcc tcttcatggc gctccgcatg
     1201 gaggacagct accccatcat cgacatcctg gagcagacgc cgcccattcc ggacaattgc
     1261 cagtgggcgc tgttcctccg taatcacgac gagctcaccc tcgaaatggt gaccgacgaa
     1321 gaacgcgatt acatgatcgc gcgctacgcc gcagatcgac agacgcgaat caacctcggc
     1381 attcggcgtc ggctcgcgcc gctcctcgac aacgaccgcc ggcgtatcga gctcatgaac
     1441 atgctcttgt tgtcgctccc cggcaccccc gtcatctact acggggacga aatcgggatg
     1501 ggtgacaacg cctacctcgg cgaccgcaac ggcgtgcgca cgcccatgca atggagcccg
     1561 gaccgcaacg gcggcttttc gcgcgcaaac ccacaaaggc tgattttacc gacaatcatc
     1621 gatccggaat acagctacga gacggtcaac gtcgaggtcc aacaaggtaa cccgagctcg
     1681 ctcctctggt ggatgaagcg cttgctcgcc ttgcgcaaaa agagcctcgc gttcgggcgc
     1741 ggaagcctgc aaatgctcgc tcccgagaac cgcaaagtgc tcgcatttct gcgccgtcat
     1801 cacaccgacg tgatcctcgt cgtgatgaac ctttcgcgct ttccgcagtg ggtcgagctc
     1861 gatctctcgg agttcaagtc acagtccccc gtcgagctgt tcggcaacgg cgctttcccg
     1921 cccatcgacg gcaaccccta tcgcctcacg ctcggagcgt acgacggttt gtggctctcg
     1981 ctgactagcg ccacgtcttc agggggaagt cagcatccct cgctcgtacc gcgccgacac
     2041 ctcgcattca acggcgacta cgcccagctg ctcaaacaag agtcggcggc gctcgacatc
     2101 gccctgaccg cgtacatccc ctcgcagcgc tggtttcgca gcaagacgct gcgcgtcgag
     2161 cgagcgaggc tgcgcgaggc gttgccgctc ggcccccggc tattcctcgc gttcatcgaa
     2221 gtcacgttcg cggagggtgc gcccgagctc tacgtgctgc cgctgggaat cgtcgagcag
     2281 acgaaagagc cggacttgcc actcgtctcg atcatcaaag acgccgactc ccctccccac
     2341 gcttgggtct acgacgcttc caacgacgcg gacaccgcgc tcgcgctcca gtcgctcgcg
     2401 ctcggtggcc gtcagctgga aacgcgcttc gtatcgatcg aggggcgccg cgtcgtcagc
     2461 ggcgaggcgc cagaggttcc gagcgcaaaa gcgctgcgtg ccgagcagag caacacttcg
     2521 tacctctacg cggatcgcta cgtcggcaag ctggttcgca aagtcgaaat tggagaaagc
     2581 ccggaggtag agctcctcgg ggcgctgcag agaggaccga agggcgcgag cgtcccgccg
     2641 ctagtcgcgc acatccaagc agagctcgcg ggcggcgtcg caaccttgat gacgctcttg
     2701 ggttacgtgc cgaacgaggg caacgcctgg gatttggctg tcgatcacgc tcagcatttc
     2761 tacgagcagg ttctcgccaa gcaccgcgac gagcctccgc cggggacggt cagcctcttc
     2821 gacgaggcga ccaaccccac cgcgatttcg agcgacttcc tcgcgctagc aaagctgctc
     2881 ggcacccgca ccgccgagct ccattcggca ttgctcgcga acttgagtgg gaccccgctc
     2941 ggtcctcgac cctacacgac cctcacgagc cgcgccttct accagtccgt acgca
//
LOCUS       DASTKS010000109         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1132251, whole genome shotgun sequence.
ACCESSION   DASTKS010000109 DASTKS010000000
VERSION     DASTKS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132251"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFQ35_02330"
     CDS             complement(<1..1164)
                     /locus_tag="VFQ35_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929489.1"
                     /translation="MRAGRFSSTRRLPAILGVVLLGSLGVSTPAWAVESTPSMAGESS
                     KIRPAGERQIGARARPTIKKKAEDKAKNQRALRVSLQIPEKLRAALALRIDDRINRNI
                     AAAKKLRGEALSLLTKFVAESNPDSAEMPEALLRVGELEWEEARDRFVSDFEKWEKTP
                     TDQRGEPPVPNYEKPRAHIRLVLTRYKSFPKYDLALYVDGFLANEEGKFDQAVKRFDK
                     IIEWFPKSRFIPDAHMVRAEYEFTRDAPNYETAYREYEEVLKYKDSPLYDLALFKSAW
                     TLWRLGKTAEAARRFLTVFKSTAEGAPTRPGQSQSDLDELQNEALKNLVAVFVEDEKN
                     RAEDMHRFLVQAGGDKFAGRIVRALAEAFFDQSHYERGIEAYRLLLKLEPTSPE"
     gene            1519..1881
                     /locus_tag="VFQ35_02335"
     CDS             1519..1881
                     /locus_tag="VFQ35_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929490.1"
                     /translation="MSEDILVVDDDPVVTRMLSRVLSAHRVVVAHSGEDALARIGTQS
                     FALILCDLSMPGMSGMAFYESVRAISPELAKRVVFMTGGAFTAESHEFLVRHENSWLE
                     KPFDIGALRKLIARAGEL"
     gene            1954..2844
                     /locus_tag="VFQ35_02340"
     CDS             1954..2844
                     /locus_tag="VFQ35_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET9929491.1"
                     /translation="MFRLDLNESVPREIRRLLLDELKDGEAALLGTEGSLAERVHEAR
                     KRIKRVRAVLALVDGGGPGAELAEACRRAAARLAGPRGHAALLHSYERLVSLEPECEN
                     AALRQELHARAASVSEGAPDFLEATARCLHDARAFAKQVEVQGNGFDAIARGFRATYR
                     RARRALHLARASGKATDFHAFRKPCKRHFYQLRLLEDVWPAVMETRTAELDRTGELAG
                     EHHDLSLLRDELKKLVPGEPGQGLDAIVARRERQLEQQILALGELCFAERGRAITSRV
                     RRYWQATAENGIVAKPTPEV"
     gene            complement(2846..>3336)
                     /locus_tag="VFQ35_02345"
     CDS             complement(2846..>3336)
                     /locus_tag="VFQ35_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929492.1"
                     /translation="RASGANKAGVEALAKELEALELRARGLRAGLNAGSSGGSGDGAD
                     LGALLARDRDRATALYGETEKLSSDLSKQQIALAQAAFQRLDKRLTRLVNRARLGRIE
                     TVLGRKRALEIEVEALSQGLLPSTIVDSLKAERYLGDNEEYWPFEGEDWADEYVGGEG
                     IR"
BASE COUNT          547 a         1095 c         1082 g          612 t
ORIGIN      
        1 ctcggggctc gtgggctcga gcttcaacaa caaccgatac gcttcgatgc cacgctcgta
       61 gtgcgactga tcgaagaagg cctccgcgag cgcccgcacg atgcgtcccg cgaacttgtc
      121 gccccctgcc tgcaccaaga agcggtgcat gtcttcggcg cggttctttt cgtcttcgac
      181 gaacaccgcg accaggttct tcagcgcctc gttctggagc tcgtcgaggt cactctgact
      241 ttgccccggc cgcgtcggcg cgccctccgc ggtcgacttg aacacggtga ggaaccgccg
      301 cgccgcctcg gccgtcttgc ctagccgcca aagggtccaa gcgctcttga acagcgcgag
      361 atcgtagagg gggctgtctt tgtacttgag gacttcttcg tactcgcgat acgcggtctc
      421 gtagttcgga gcgtcccgag tgaactcgta ctccgcccgc accatgtgcg cgtcgggaat
      481 gaagcggctc ttcgggaacc actcgatgat cttgtcgaag cgcttcaccg cctgatcgaa
      541 cttgccctcc tcgttcgcga ggaagccgtc gacgtagagg gccagatcgt attttggaaa
      601 gctcttgtag cgcgtcaaca ccaagcggat gtgcgcgcga ggcttctcgt agtttgggac
      661 cggcggctcg ccgcgctggt ccgtcggcgt cttctcccac ttctcgaaat cgctcacgaa
      721 gcgatctcga gcttcttccc actcgagttc gccgacgcga agtaacgcct ccggcatctc
      781 ggccgagtcg gggttcgact ccgcgacgaa cttcgtgagc agcgagagcg cttcgcccct
      841 caacttcttc gcggccgcga tgttacggtt gatgcgatcg tcgatccgca gcgcgagcgc
      901 ggcccggagc ttctccggga tttgcaggct gacgcgcagc gcccgctggt tcttggcctt
      961 gtcttcggcc ttcttcttga tggtgggccg ggcccgcgca ccgatctggc gttcaccagc
     1021 gggacgaatc ttcgacgact cgcccgccat actcggcgtc gattcgacgg cccaagcggg
     1081 cgtactaacc ccgaggctgc ccaacagaac cactcccaag atcgccggca gacgtcgagt
     1141 gctcgagaac cgaccagctc gcatgtgaag ccgagaataa ctgacgggtg agaccaggag
     1201 caatcggccg cgcacgaagt cgggaaccgc tcgcgctgct cgctcgatcc agcttcgcgc
     1261 tccacgggaa aatcggccgg accgaccccg aagtactgtg cgcggagtca ggcagcgaca
     1321 atgtcgtgtc gcgtctcacc ctcaccaaag tccggcctgt cgaggcattc gcgcccgcgt
     1381 cgtgtctcga tcgaagacgc gttctcgtcg ggacgagatt gccggaaaca gcggcgggag
     1441 gcttccttca gctcgagcgt tggcctatgc ttgaccttag cgtgaagcgg ttcggaccgg
     1501 cggcacgcgg aggaggccat gagcgaggac atcctcgtcg tcgatgacga cccagtcgta
     1561 acgcggatgc tgagccgcgt cctctccgcg caccgggtcg tggtcgctca ctcgggcgaa
     1621 gatgcattgg cgcgcatcgg gacgcagtct tttgcgctga ttttgtgtga cttatcgatg
     1681 ccgggaatga gcgggatggc gttctatgag tccgtgcgtg ccatctcccc cgagctagca
     1741 aagcgcgtcg tgttcatgac cggcggggcc ttcacggccg agagccatga gttcttggtg
     1801 cgtcacgaga actcttggct ggagaagcca ttcgacatcg gagccctgcg gaaactcatc
     1861 gcacgcgccg gtgaactctg agggcggcga gttgcgacgc cggtgcggtg tcgtatggtc
     1921 ggagggctct cctggcttgg ccggggtctc tccatgtttc ggctcgacct gaacgaatcg
     1981 gtaccccgcg aaatccgccg gctcttgctc gacgagctga aggacggtga agcggcgctg
     2041 ctcggtaccg agggctcgct cgccgagagg gtgcacgagg cacgcaagcg tatcaagcga
     2101 gtgcgcgcgg tgctcgcgct ggtcgacggc ggtggcccag gggcagagct cgccgaagcc
     2161 tgccgacgcg ccgcagcacg gctggcaggc cctcgcggcc atgcggcgtt gctgcacagc
     2221 tacgagcgac tggtctcgct cgaacccgag tgcgaaaacg cggctctccg ccaggagctt
     2281 cacgctcgcg cggcttcggt cagcgagggc gcgcccgact ttctcgaagc tacggcccgc
     2341 tgtctgcacg acgcgcgcgc gttcgcaaag caagtcgagg tgcaaggcaa cgggttcgac
     2401 gcaatcgcgc gcggctttcg agcgacgtac cggcgcgcgc gtcgagcact gcacctcgcc
     2461 agagccagcg gaaaggcgac tgactttcac gcttttcgca agccgtgcaa acggcacttc
     2521 taccagctcc gcttgctcga agacgtgtgg cccgcggtga tggagacgcg caccgccgag
     2581 ctggatcgca cgggagagct cgcgggagag caccacgacc tcagcctcct gcgcgacgaa
     2641 ttgaagaagc tcgttccggg cgagccgggg caaggcctcg acgcgatcgt cgcgcgccgc
     2701 gagcgtcagt tggaacagca gatcttggca ctcggcgagc tttgtttcgc cgagcgcggt
     2761 cgcgccatta cgtcgcgtgt acgccgttac tggcaggcga cggccgagaa cggcatcgtc
     2821 gcgaagccga cgccggaagt gtagctcagc ggatcccttc gccgccgacg tactcgtccg
     2881 cccagtcttc gccttcgaag ggccaatatt cttcgttgtc gccgaggtaa cgctcggctt
     2941 tgagcgagtc gacgatcgtg ctcggcaaga gaccttggga gagcgcttcg acctcgattt
     3001 ccagggcgcg cttccgcccg agcaccgtct cgatgcgccc caagcgcgcg cgattgacga
     3061 gccgagtcag gcgcttgtcg agacgctgga acgcggcctg agccagcgcg atctgctgct
     3121 tggataggtc cgacgagagc ttttcggttt cgccgtagag cgcggtcgcg cggtctcgat
     3181 cgcgagcgag caacgcgccg aggtcggcgc cgtcgccgct ccccccactg ctgccagcgt
     3241 tgagcccagc gcgcaagccg cgcgcccgga gctcgagcgc ctcgagttcc ttcgcgagcg
     3301 cctcgacgcc ggctttgttg gcgccgctcg ctcgct
//
LOCUS       DASTKS010000110         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_463855, whole genome shotgun sequence.
ACCESSION   DASTKS010000110 DASTKS010000000
VERSION     DASTKS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463855"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1200
                     /locus_tag="VFQ35_02350"
     CDS             355..1200
                     /locus_tag="VFQ35_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9929493.1"
                     /translation="MCPRDIHRSFEQGASADATSRHNVVFWGSGSKPLVFAHGFGTDQ
                     RVWRFVEPEFRADYRTVLFDHAGCGRALPSAYDSRRHADLRGYAMDLVDICRAVASEP
                     VVFVGHSVATMIGILASIEAPELFGRLVLVAGSARYLNEPPHYHGGFDESDVKSLLDM
                     MDQNFLGWATTFAGIAAKDPSTAEELFTSFCSADPRTIRHFAEVTFRGDVRDELCRVT
                     VPSLLMQCEYDDIVPAGVSEYMRERLDRCELEVLPIAGHCPQVSAPELVVSALKRFLL
                     NSECA"
     gene            1191..2354
                     /locus_tag="VFQ35_02355"
     CDS             1191..2354
                     /locus_tag="VFQ35_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929494.1"
                     /translation="MRLNSPIYGFVGYVVCDDRNLIVEVNAEASRLLEYAPEELRGKP
                     LASILDKPAQAFFQMQVWSTLAQQGRLEEAYVSLLTKDGSRLPVLLNAGRVAQDSVSE
                     NHLVFFPMRRRNLFERELVEAQAAAERARQAEQAALARVHGMQVKLALQERLATIGTL
                     VAGVIHELNNPLLYLGSNLELIEEELRAPRADAASVLEMLKDAREGAAQIGKLVKSLR
                     VYAGGNGGSVGPVELSNVVAAAVRMTTHAVKRRALTSVQIEAPAATVLGDETRLTQVL
                     INLLLNASQAFERADPQRNSIRVRVQRDGARYAEVCVADNGPGVPPELRRRIFEPFFT
                     TKPVGEGTGLGLAICADIVASMSGELDVESEPGEGACFRIKLPLLEKSVRSEG"
     gene            complement(2444..>2633)
                     /locus_tag="VFQ35_02360"
     CDS             complement(2444..>2633)
                     /locus_tag="VFQ35_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929495.1"
                     /translation="QEVYEREGDVNNVVFPCGYTIGADGDALNLYYGGADSVIALCQT
                     SIRELLGWLTEYGVTEGL"
BASE COUNT          472 a          807 c          861 g          493 t
ORIGIN      
        1 tgagcgaata ggcgacccgc ctgaaatcgc ggcattttgc ggctcggagg cagacggagg
       61 ggcccttgtc gacccgggtg gggcatctcg cggcccgcag tggtctcccg tgatttacga
      121 cgggcgcggg gcggagtgtc cgcgagcggc gcaggggttc gtcatcaatt cgacgggctc
      181 gtgttctgga cgaagtggac ggcgggccag cgagcacgca gcggtacagg ccggcagccg
      241 tggcccgctc tgaagaactt ggcggctctc gccactgcaa aggcgatcgc atcgctcgag
      301 ggtgtgcagt agacttccaa tcccggcacg cattcgcatt cgcgagggga aacgatgtgc
      361 ccgcgagaca ttcatcgatc attcgagcag ggagcatcgg cggacgctac gtcacggcac
      421 aatgtggtgt tttggggcag cggcagcaag cccttggtgt tcgcgcacgg gttcgggacc
      481 gatcagcgcg tgtggcgctt cgtcgagccc gaattcaggg ctgactacag aaccgtcctc
      541 ttcgaccacg ccggttgtgg gcgcgctcta ccgtcggcat acgattcgcg aaggcatgcg
      601 gaccttcgtg ggtacgcgat ggacctagtc gatatttgcc gcgccgtcgc gtcggagccg
      661 gtggtcttcg ttgggcactc ggtggcgacc atgattggga tcctagcctc tatcgaggct
      721 cccgagctgt tcgggcgctt ggtgctcgtc gctggttccg caaggtacct gaacgagccg
      781 ccgcactatc atggaggctt cgacgagtcg gacgtgaaat ctcttctcga catgatggac
      841 cagaatttct tgggctgggc cacgacgttt gcaggcatcg ccgcgaagga cccttcgaca
      901 gcggaagagc tcttcacgtc gttctgtagc gcagatcccc gcacgattcg tcattttgcc
      961 gaggtgacct ttcgaggcga cgtccgcgac gagctttgcc gggtcaccgt tccgagcctg
     1021 ctcatgcaat gcgagtacga cgacatcgtc cccgctggcg tgagcgagta catgcgcgag
     1081 cggctcgatc gctgcgagct cgaggtgctg ccgatcgccg gacactgtcc tcaagtgagc
     1141 gctcccgagc tcgtcgtctc ggccctgaag cgcttcttac tgaactcgga atgcgcttga
     1201 attccccgat ttacggcttc gtgggctatg tcgtttgcga cgaccgtaac ctcatcgtcg
     1261 aggtgaacgc cgaagcgagc cgcctactcg agtacgctcc cgaagagctc cgcggaaagc
     1321 ctctcgcctc gatcctcgac aaaccagcgc aggcattctt ccaaatgcaa gtatggagca
     1381 cgctcgcgca gcagggacgg ctcgaggaag cctacgtctc gctcctgacg aaagacgggt
     1441 cgcggcttcc cgtgctcctg aacgcgggac gggtcgcgca ggattccgtt tccgaaaacc
     1501 acctcgtgtt cttcccgatg cggcgcagga accttttcga gcgcgagctc gtcgaggccc
     1561 aggctgcagc ggaaagggct cggcaagccg aacaggctgc gctcgcgcga gtgcacggca
     1621 tgcaagtcaa attggctcta caagagcggc tcgcgacgat tgggacgttg gtggcgggcg
     1681 tgatccacga gctcaacaat ccgctcttgt atctgggcag caacctcgag ctaatcgaag
     1741 aggagctgcg cgcgcctcgg gccgacgcgg cttccgtcct cgagatgctg aaggacgcgc
     1801 gtgaaggtgc cgcgcagatc ggcaagctcg tcaagagcct gcgcgtgtac gcaggcggta
     1861 atggcggatc ggtgggtccg gtcgaactct cgaacgtcgt cgctgccgcg gtgcgaatga
     1921 cgacccacgc cgtcaaaagg cgcgcgctca cctcagtgca aatcgaagcg ccggccgcga
     1981 ccgttctcgg agacgagacg cgtctgactc aggtcttgat aaaccttctc ttgaacgcgt
     2041 cgcaagcgtt cgagcgtgcc gacccgcaac gaaatagcat tcgagttcgc gttcaacgtg
     2101 acggcgcccg ctacgcggag gtttgcgtcg ccgacaacgg ccccggggtg cctcccgagc
     2161 tccgtcgacg catcttcgag ccgttcttca ccaccaagcc ggtcggcgaa ggaaccggcc
     2221 tcggcttggc gatttgcgcc gacatcgtcg cgtcgatgag tggcgagctc gacgtggaga
     2281 gcgagcctgg cgagggcgct tgctttcgca tcaagttgcc attgctcgag aagagcgttc
     2341 ggagcgaagg ttgagcgaag aggggcttcg ttcaatcgcg agcgcgggcg gcgtcgtgtc
     2401 gcgcgagcac gagggatgac gaacacggag cgttcgccgg cgctcagagc ccctcggtaa
     2461 cgccgtactc ggtgagccag ccgagtagct cgcggatgct cgtctggcac aaagcaatga
     2521 cgctgtccgc cccgccgtag tagaggttca aagcgtcgcc gtcggccccg atcgtgtacc
     2581 cacacggaaa gaccacgttg tttacgtcgc cttcgcgctc gtaaacctcc tgt
//
LOCUS       DASTKS010000111         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_238875, whole genome shotgun sequence.
ACCESSION   DASTKS010000111 DASTKS010000000
VERSION     DASTKS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238875"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFQ35_02365"
     CDS             <1..449
                     /locus_tag="VFQ35_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HET9929496.1"
                     /translation="PVWRALEGRRGTVLEVCGSLENVELAEYVHTFLNHTAVALWKDY
                     KAKRRVRGDTGHHSYVSGVMSGFRDKLASEKRRHNEEGLIWLGDPELGRYLRERHPRI
                     RHTSYKSHGRTEAYAEGRAAGRNIVLHRGVNSGPSGGKTRLLGPAR"
     gene            524..904
                     /locus_tag="VFQ35_02370"
     CDS             524..904
                     /locus_tag="VFQ35_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HET9929497.1"
                     /translation="MQRRVLPESRVHPSIHDKLGSQGSSVVDEVEALIQKHPVVVVGM
                     SQNPFPRRARKLLDSQKIAYEYVGYGSYLSQWRKRLPLKLWTGWPTFPMIFVNGTFIG
                     GYEDLRRLVSSGELRALLTGPRPS"
     gene            966..2102
                     /locus_tag="VFQ35_02375"
     CDS             966..2102
                     /locus_tag="VFQ35_02375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9929498.1"
                     /translation="MLRAIGALSLLSLLLLPACSSSESSGNANGGRGGVSGAGGNGGS
                     GGASTCAAPPSLGVRWVGRVDGCDPRGARYAWSGSGFVGRFSGTGLGVRLLDAGQQHT
                     VLIDGALQPTLKTQIADAMYPLASGLPQGEHTFEVYRRTEASFGETIVASFAVTGGEL
                     LSPPEPPARRIEVVGDSITCGYGNEGTLPCMFSADTENHYLSYAAIAARDLGAELSTV
                     AWSGKGVVFNYNGDPNSPLPTLYSLSSPTDRESTWSFAWQPDAVVINLGTNDYSTGKN
                     PTDAMFVEAYTGLLARIRGVYPKAFVLCTMGPLLNGSGLATARKNISGAVEARHAAGD
                     QQVKYFEFATPNGMPPGCDYHPNLATHDAMAKELAAELRADLSW"
     gene            2222..>3479
                     /locus_tag="VFQ35_02380"
     CDS             2222..>3479
                     /locus_tag="VFQ35_02380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929499.1"
                     /translation="MPELTDSLPEQEDLVGSVLAGRYRVVALLGAGGMGSVYRAEHVH
                     MRKAVAVKVLHREMTSVPEVVRRFEREAVAAARIEHPNVATATDFGSLENGSFYLVLE
                     FIEGQSLGALIKAEGALDEARTLRIGRQIADALVAAHAAGIVHRDLKPDNVMLVDREH
                     ASDFVKVLDFGIAKVKLEASSDQPLTQMGMVFGTPEYMSPEQARGLDVDARSDLYALG
                     LILYELLSGASPFRHEDLVAVLTRQITMNPDPLPGHVSAGTSELVMRLLRKDPAERLQ
                     TALEVREALDSLLAGSSGLRAQAPTEPVTAPKSAPQVAAVTPPPAVAPAKNLVVLADG
                     RTALALPALTQAPLRVLAAWVRRLGLERRVRIGRYGVPLGTVLGLSLLVLVAFGLSLV
                     LLAVHPTATPEVGTLPPRPQDPDVLTL"
BASE COUNT          584 a         1155 c         1156 g          584 t
ORIGIN      
        1 taccggtttg gcgggcgctc gagggacgcc ggggcacggt gctcgaggtc tgcggctcgc
       61 tcgagaacgt cgaactcgcg gaatacgtgc acacctttct gaaccatacc gctgtcgcgc
      121 tctggaagga ctacaaggcc aagcggcgcg tacgaggcga cacgggacac catagctacg
      181 tgtcgggcgt aatgagcggt ttccgcgaca agctggcgag cgaaaagcgc cgacacaacg
      241 aagagggcct gatttggctc ggcgatcccg agttggggcg gtacctgcgc gagcgccacc
      301 cacggattcg gcacacctcg tacaagagcc acggccgcac cgaagcttac gccgaggggc
      361 gcgccgcggg caggaacatc gtgctgcacc ggggcgtcaa ctccgggccg agcggcggca
      421 agacacggct actcggaccc gcgcgctgag gtgtgcgccg cgtggtcgga tcgcgagagt
      481 gaggccttgc gggctcttcg cttcgaccac atatcgctgc cccatgcagc gcagggtctt
      541 gcccgagtca cgtgtccatc cttcaatcca cgacaaactg gggagccagg gctcgagcgt
      601 ggtcgacgag gtcgaggccc tcatccaaaa gcacccggtt gtggtcgtgg gcatgagtca
      661 gaacccgttt ccgcgccggg ctcgtaagct gctcgacagc cagaaaatcg cctacgaata
      721 cgtgggatat ggaagctacc tttctcaatg gcgcaagcgc ctgccgctga aattgtggac
      781 aggttggccg acgttcccga tgatcttcgt gaacggcacg ttcatcggcg gatacgagga
      841 cctgaggcgc ctcgtctcga gcggcgagtt gcgggctctg ctcaccgggc cgcgcccgag
      901 ctgatgttgt cgggaaccgc gagctttgct ttacgcgcgc gagtgagtcg cctagaagct
      961 cacccatgtt acgggctatc ggtgcgcttt cgcttctatc tctcctccta cttccggcgt
     1021 gcagctctag cgagtctagc ggcaacgcga acggcggccg cggcggcgtg agcggagcag
     1081 gcggtaacgg cggcagcggc ggcgcgagca cgtgcgcggc gcctccttct ctgggcgtgc
     1141 gctgggtagg tcgagtcgat ggctgcgacc cccgcggggc acgctacgcc tggtcgggta
     1201 gcggcttcgt cggccggttc agcggtacgg ggctcggcgt tcgcctgctc gatgctgggc
     1261 aacagcacac cgtgctcatc gacggcgcgc ttcagcccac tttgaagacc caaatcgcgg
     1321 acgcgatgta cccgctcgct agcggcctgc cccaaggcga acataccttc gaggtatatc
     1381 gtcgaaccga ggcgtcgttc ggcgagacca tcgtcgcgag cttcgcggtc accggtggtg
     1441 agctcttgag ccctccagag ccacctgcgc ggcgcatcga ggtcgtcggc gattccatca
     1501 cttgcggcta cggcaacgag ggtacgctcc cgtgcatgtt cagcgcggac acggagaacc
     1561 actatctctc gtacgcggcc atcgccgcgc gcgacctcgg cgccgaactc agcacggtgg
     1621 cgtggtctgg taagggcgtc gtcttcaact acaacggcga tccgaactca ccgcttccga
     1681 ccttgtactc gctgtcttcg ccgaccgatc gcgaaagcac ctggtctttc gcgtggcagc
     1741 cggacgcggt ggtgatcaac ctcggcacca acgactacag cacgggaaag aatccgaccg
     1801 acgcgatgtt cgtcgaggca tacaccgggc tcctcgcgcg aattcgcggc gtctatccga
     1861 aggcgttcgt gctctgcacg atgggtccgc tcttgaacgg cagcggtctc gccaccgcgc
     1921 gcaagaacat cagcggcgcg gtcgaggcgc ggcacgccgc cggtgaccag caggtgaaat
     1981 acttcgagtt cgcgaccccg aacggcatgc ctccgggttg cgactatcac ccgaacctcg
     2041 caacccacga cgcgatggcg aaggagctcg cggccgagct tcgcgccgac ctgagctggt
     2101 gacagcgtcg cgtccggcgt aaccgcgcgt cgccgagagc cgaacggctt tggctgacgg
     2161 gccgcccgaa gcctttttcg gccctcgacg ctcgtaccaa cgccccgcag aatggagggc
     2221 cgtgccagag ctcaccgaca gcctacccga gcaagaggac ctggtcggca gtgtgctcgc
     2281 cgggcgctac cgcgtcgtcg ccttactcgg cgcgggaggg atgggctccg tctatcgcgc
     2341 ggagcacgta cacatgcgca aggcagtcgc ggtgaaggtg ctgcatcgcg aaatgacgag
     2401 cgtcccggag gtggtgcgcc gcttcgaacg cgaggcggtg gccgcggcgc gcatcgagca
     2461 cccgaacgtc gcgaccgcga ccgatttcgg tagcctcgag aacggctcct tctacctcgt
     2521 gctggagttc atcgagggcc agagcttggg cgctctcatc aaggccgaag gcgcgctcga
     2581 cgaagcgcga acgctgagga tcggccgaca gatcgcggac gcgctggtcg ccgcgcacgc
     2641 agcggggatc gtgcatcgcg atctgaaacc cgacaacgtc atgctggtcg atcgtgagca
     2701 tgcgagcgac ttcgtgaagg tcctcgattt cggcatcgcc aaggtcaagc tcgaggcgag
     2761 cagcgaccag ccgctcacgc aaatgggcat ggtgttcggg acccccgagt acatgtcgcc
     2821 agagcaggca cgcgggctcg atgtcgacgc gcgctcggat ctgtacgcgc tcggtctcat
     2881 cctctacgag ctgctgagcg gtgcctcgcc gttccgccac gaggatctgg tcgccgtgtt
     2941 gacgcggcag atcacgatga accccgatcc gctcccgggg cacgtctcgg cgggcacttc
     3001 ggagctcgtg atgcggctcc tacgaaaaga tccggccgag cgcttgcaaa cggcgctcga
     3061 ggtgcgcgag gcgctcgact ccctgctcgc cggctcgtcg ggccttcgag cgcaggcgcc
     3121 gacagagccc gtaaccgcgc ccaaatctgc gccgcaggtc gctgccgtca cgccaccacc
     3181 agccgtcgcg ccggcgaaga acctcgtcgt gctcgccgac gggaggacgg cgctcgcgct
     3241 gcctgcgctg acacaggcgc cgctgcgagt gctcgcggct tgggttcggc gcttgggtct
     3301 cgagcggcgc gttcgaatcg gtcgttacgg agtgccgctc gggacggtgc tcggcctctc
     3361 gctcctcgtg ctggtcgcct ttggtttgtc gctcgtgttg ctcgccgtgc acccgaccgc
     3421 gaccccagag gtcggaacgc tgccgccacg cccccaagat cccgatgttc tcaccctca
//
LOCUS       DASTKS010000112         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_239159, whole genome shotgun sequence.
ACCESSION   DASTKS010000112 DASTKS010000000
VERSION     DASTKS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239159"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..219)
                     /locus_tag="VFQ35_02385"
     CDS             complement(31..219)
                     /locus_tag="VFQ35_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929500.1"
                     /translation="MTSIPPSYTWIAAFAVGFGLAALSRLWEKHRGARKAPAHCKADA
                     YARTALTPPRCREAPSCS"
     gene            complement(370..1392)
                     /locus_tag="VFQ35_02390"
     CDS             complement(370..1392)
                     /locus_tag="VFQ35_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929501.1"
                     /translation="MRRAESNKKPGISYAFTNDGLELPLIDVTHPAFALPRGTELQRK
                     AVQKFVRDQKQFQKLPTLAQKLLLRLFLRGSLHTRALRDAASTFLPGLPTYLLKIPPD
                     LLGEYARPIDRKLAEGAPILSVRYRLQDMATLAAEALAPALLAERERPLVLVNIAGGP
                     AFDSLNALLLLRRDHPELLRSRSTRIRVLDADSAGPQFGARSLEVFASPGAPLEGLDI
                     RFDYERFDWRDRAGLRAVLESAATQNAVTLGQSEGGLFEYGSDADIVAVLSEFREAAR
                     GFLGFVGSVTRNDEFVAFAKSISKTTTRPRGLSVFEPLCRAAGYEIHRALERPLSDNV
                     WIRPAE"
     gene            1557..2447
                     /locus_tag="VFQ35_02395"
     CDS             1557..2447
                     /locus_tag="VFQ35_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HET9929502.1"
                     /translation="MRSMRAGQNARSPTEVAPRRRERYTTTGFTGVSEALSRLLVSTA
                     PEAQLLSKQTSSTHSRVRMAMLSGIAGFVDAAGFASVAGLFPAHITGELVGAVAGLSS
                     GSWQREAARIAVLIVFVMTVVLGALISRSLRKRGQSPLAALFGLMSLGLLVASVSDAF
                     GSLLGAEAAAFSSAAMKCGLVGAMALQNVVMRHALSSSCPTTVMTGNLTQFIIEFVEL
                     IVGRGEKTERSLVRSKSEIRLRVLGTALGAFLASAALSVFLMTKIGSLGILIPTFAIG
                     SLALRELKYPTAVSRTGTLY"
     gene            2560..3726
                     /locus_tag="VFQ35_02400"
     CDS             2560..3726
                     /locus_tag="VFQ35_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929503.1"
                     /translation="MKAAPTHRDELPMLERWGLAVLRGAPREPPVASADDPIHVLNPD
                     ERRALRRIERDAVLRAALAGSLSGLVSASAAAWAHKFLGPDGAPHSLEHALKFWGTVG
                     VATAFASIFEIGFLYWDALRSVHRMAGAAGLALTEQTVSREQRDVALALARAALELPN
                     PPHPELPLDPRRESVRWLAALAALLYKAKVALTNVALKAGLRAFIGRSITLVALEFVV
                     VPVTAIWNAVVCWSVVREARLRAMGPSAAIELVSLALADISPTDAGREAALRAVASAV
                     VRTRDLHPNHLAIIRALGAQFEPSGCAELDDSNRFLARLAELPATDQRLALRLLVIAA
                     ILDGRLTRAEKRLLEQAFAACGRTVSLASVERLRKALAAGDEFEFSEVRALTDG"
     regulatory      3825..3993
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4135..>4426
                     /locus_tag="VFQ35_02405"
     CDS             4135..>4426
                     /locus_tag="VFQ35_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929504.1"
                     /translation="MKALTLGFLAVALCVSVGCGDDDDGNGAAGGNGGAAQGGKAGAG
                     SGGKTSGGANAGGDGGVGGSNSDGGVAGAPITPPPVMCEPPEPPLFGAGGASS"
BASE COUNT          686 a         1414 c         1524 g          802 t
ORIGIN      
        1 ccgacttcag cggcttcggc gaccccaagc tcacgagcaa gacggcgctt cccggcatct
       61 tggcggggtg agcgcagtgc gggcgtaggc gtcggccttg cagtgggccg gcgccttgcg
      121 cgcgccgcgg tgcttctccc agagccgaga gagcgcagcg agcccgaagc cgaccgcgaa
      181 ggcggcgatc caggtgtagc tcgggggaat tgacgtcatg gcttcgcaca tcggtcgtct
      241 gacagtccgt gcccggtgac gttgcgaccg ttcaacgcgc cggttccttt tcgagtcgca
      301 cacggacggc tggtccacaa tcccgcgatg gaggggttga ctttcgcggg cggcctcggg
      361 acgacgaggc tactccgcgg gccgaatcca gacgttgtcg ctcagcggcc tctcgagtgc
      421 gcggtgaatc tcgtatcccg cggcgcggca gagcggttcg aatacggaga gaccgcgcgg
      481 tcgcgtcgtg gtcttgctga tcgatttggc gaacgcaacg aactcgtcgt tgcgcgtgac
      541 gctccccacg aagccgagga aaccgcgcgc ggcttccctg aactcgctga gcaccgcgac
      601 gatgtcggcg tcggagccgt actcgaacaa gccgccctcc gattgaccga gcgtgacggc
      661 gttctgcgtc gcggcgcttt cgagcacggc gcgcaagccc gcccgatccc gccagtcgaa
      721 gcgctcgtag tcgaaccgga tgtccaaccc ctctagggga gcgccgggcg acgcaaaaac
      781 ttcgagagag cgcgcgccaa attgcggacc cgcgctgtcg gcgtccagca ctcgaatgcg
      841 ggtgctccgc gagcggagca actccgggtg atcgcgacgc aacaacaaga gcgcattcaa
      901 gctgtcgaat gccgggccgc ccgcgatgtt gacgagcacg agcggtcgtt cacgctctgc
      961 aagaagcgcg ggtgccaacg cttcagccgc gagggtcgcc atgtcctgca ggcggtatcg
     1021 caccgacaaa atcggtgcgc cctcggcgag cttgcggtcg attgggcgcg catattcgcc
     1081 caacagatcc ggcgggatct tgagcaggta cgtcggcaag cccggtagga aggtcgatgc
     1141 cgcgtcgcgg agcgcacgcg tatgcagcga gccgcgcaag aagaggcgta gcaatagctt
     1201 ttgcgcgagc gtggggagct tttgaaactg cttctggtcg cgcacgaact tctgcacggc
     1261 cttgcgttga agctcggtgc cgcgcggcaa cgcgaacgcg gggtgcgtca cgtcgatcag
     1321 cggaagctcg agtccgtcat tcgtgaaggc gtacgaaatg cccggcttct tgttcgattc
     1381 ggcgcgtcgc atgccgcgga acgttacgca aacgagtggg tttagtaaaa ccgcgcgtca
     1441 ctctctcgag tgtcgatgct gtccagggtg aactcgtgtg acacggtaac ccccgcggca
     1501 tcccatgttg ggaagcgtga tttcacacta aagcgtgggg tggaacgagc cgttctatgc
     1561 gctccatgag ggcagggcag aacgctcgct caccgaccga agtcgcgcca agacgacgtg
     1621 agcgctatac gacgaccgga ttcacgggtg tttctgaggc tctttcgcgg ctcttggtgt
     1681 cgacggcgcc agaggcccag cttctctcga agcagacgag cagcacgcac agtcgcgtgc
     1741 gaatggcgat gttgagcggc attgccggtt tcgtggatgc ggcgggattc gccagcgtcg
     1801 ctggtctctt tcctgcgcac atcaccggag agttggtagg ggccgtggcc ggcttgtcat
     1861 ccggcagctg gcagcgcgag gcggcgcgaa tcgccgtctt gatcgtgttc gtgatgacgg
     1921 tcgtgctcgg cgctctgatc tcgcgctcgt tgcgcaagcg cgggcagtcc ccgctcgcgg
     1981 cgttgttcgg tttgatgtcg ctcgggcttt tggtggcgag cgtgagcgac gccttcggtt
     2041 cgctgctggg ggccgaggcg gcagcgttct ccagcgctgc catgaagtgc gggctcgtcg
     2101 gagcgatggc gcttcagaac gtcgtcatgc gccacgcgtt gagcagctcg tgcccgacga
     2161 cggtcatgac cggcaacctg actcagttca tcatcgagtt cgtcgaactc atcgtgggcc
     2221 gcggcgaaaa gacggagcgc tccctggtcc gctcgaagtc ggagattcgt ctgcgcgttc
     2281 tcgggacggc gctcggcgcg ttcctcgcgt cggcggcgtt gagcgtgttc ctgatgacga
     2341 agatcggctc gctcggcatc ttgatcccca cgtttgcgat cggcagcctc gcccttcgcg
     2401 agctgaagta tccgaccgcg gtgagtcgga ccggcacgct gtactgactt ttcgtccccg
     2461 ttggcggcct cggcgcagcg tacgcgccgg aggcacgctg gtcttcccgc tagatctgct
     2521 ctaagaggtc gaacgcgcct tggcccgcgc ccccgaccca tgaaagctgc acctacccac
     2581 cgcgacgagc ttcccatgct cgagcgttgg ggactcgcgg tgttgcgcgg cgctcctcgg
     2641 gagccgccgg tcgcgagcgc cgacgatccg attcacgtgc tgaacccgga cgagcgtcgc
     2701 gcgctccgtc gcatcgagcg cgacgcggtg ctcagggccg cgctggcggg ctcgctctct
     2761 ggtctggtga gcgccagcgc cgcggcgtgg gcacacaagt tcctgggccc tgatggggcg
     2821 ccgcactcgc tcgagcacgc gctcaaattt tggggtacgg tcggcgtcgc gaccgcgttc
     2881 gcatccatct tcgaaatcgg tttcttgtac tgggatgcgc tgcgttcggt gcaccgcatg
     2941 gccggcgccg cgggcctcgc gctgacggag caaacggtat cgcgcgagca acgcgacgtc
     3001 gcgctcgcct tggcgcgcgc agcgctggag cttccgaatc ctcctcatcc ggagctaccg
     3061 ttggaccctc ggcgcgagag cgttcgctgg ctcgccgcgc tcgccgcgct tttgtacaag
     3121 gccaaggtgg cccttacgaa cgtcgcgctg aaggcgggcc tgcgcgcgtt catcgggcgg
     3181 tccataaccc tggttgcgct cgagttcgtg gtcgtgcccg tgaccgcgat ttggaacgcg
     3241 gtggtgtgct ggtcggtcgt acgcgaagca cgtctgcgcg cgatgggtcc ctcggcggcg
     3301 atcgagctcg tgagcctcgc cctcgccgat atctcgccga cggacgccgg acgcgaagcg
     3361 gcgctgcgag cggtagcgag cgcggtcgtt cgcactcgcg acctgcatcc gaatcacctc
     3421 gccatcattc gcgccctcgg cgcgcagttc gagccttccg gttgcgctga gctcgacgac
     3481 tcgaaccggt tcctcgcgcg gctcgccgaa cttcccgcga ccgatcagcg gctcgcgctg
     3541 cgcttgctcg tcatcgctgc catcttggac ggtcggctca cgcgtgccga aaagcgcttg
     3601 ctcgagcaag ccttcgccgc gtgtggtcgc acggtttcgc tcgcctcggt cgaacgtctt
     3661 cgaaaggcgc ttgcggcggg ggacgagttc gagttctccg aagtccgagc gctcaccgac
     3721 ggctgagccg acgtgtggct agtttgcaga tgcgcgcgcg cggagcggac ggttttcgct
     3781 ggctaagctc ataaccccgt gttatgcgcg ctccgacggt cgattcaggc cgtcactcga
     3841 gcagagccaa gggaagccgg tgtgataccg gcgcggcccc cgccactgta accgccgacg
     3901 aacgcagtca aatccactgg gcgaagagcc tgggaaggga ctgccgagga tgacgcggaa
     3961 gccaggagac cttctgctcg agtcgagtcc ggtttgccgg acgcttttgc acccgcgggg
     4021 cgcggggtcg caaatcgaga gcgtcgtctt gcgtgactgc gaggcgactg ccggagccgt
     4081 tcctcgagag cggctctcag ctgtcgcgcc ccgactttca cataggactc gttcatgaaa
     4141 gctctgacgc tcggatttct cgcggtagca ctttgcgttt cggttgggtg cggcgacgac
     4201 gacgatggca acggtgcggc cggtggaaac ggcggcgcag cgcaaggcgg caaggccggc
     4261 gcggggagcg ggggtaaaac ttcgggtggc gcaaacgcgg gcggcgatgg cggcgtcggc
     4321 ggatcgaaca gcgacggcgg cgtcgcgggc gcgcccatca ctccgcctcc cgtcatgtgt
     4381 gagccaccag aaccgcctct cttcggcgcg ggcggtgcat cgagct
//
LOCUS       DASTKS010000113         8411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1133107, whole genome shotgun sequence.
ACCESSION   DASTKS010000113 DASTKS010000000
VERSION     DASTKS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133107"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..556
                     /gene="kdpF"
                     /locus_tag="VFQ35_02410"
     CDS             464..556
                     /gene="kdpF"
                     /locus_tag="VFQ35_02410"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HET9929505.1"
                     /translation="MSTLYALAGIVALGLLVYLSIALLKPETFS"
     gene            553..2295
                     /gene="kdpA"
                     /locus_tag="VFQ35_02415"
     CDS             553..2295
                     /gene="kdpA"
                     /locus_tag="VFQ35_02415"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HET9929506.1"
                     /translation="MTTNDLLQIGIFFAVLLALALPLGLYMARVYEGRRLALEKVFGP
                     VERLIYRLSGVKPDSEMDWKTYAVAMLAFNMVGLLAVYALQRVQGALPLNPQGLPGTT
                     PDLAFNTATSFATNTNWQSYGGESTLSYLTQMLGLTVQNFVSAASGMAVLVALIRGFT
                     RRESTTIGNFWVDLVRGNLYILLPLSVVTALLLVSQGVVQTFAPYREVPLLQALQGEA
                     GKAPLTQLIALGPAASQIAIKQLGTNGGGFFNVNSAHPFENPTAFSNLVEVVAILLVA
                     VALCFTFGKMVKDMRQGWALLAAMMLIFVPLLLLCVGAEQAGNPALRALGVDQLASAL
                     HAGGNMEGKEVRFGIANSGLWTAATTAASNGSVNAMHDSLTPLGGLAPLWLIQLGEIV
                     FGGVGSGLYGMLVFAIVAVFVAGLMVGRTPEYLGKKIEVYEMKMAALVILLPATIVLL
                     FTAIGVSTAAGKAGIANPGAHGFSEVLYAFSSAANNNGSAFAGLAANTPFYNTLLGVA
                     MFAGRYYIKIAVLALAGALAQKRLVPASSGTLPTHTPLFVGWLTATIVIVGALTFIPA
                     LALGPVVEHLRLHS"
     gene            2304..4454
                     /gene="kdpB"
                     /locus_tag="VFQ35_02420"
     CDS             2304..4454
                     /gene="kdpB"
                     /locus_tag="VFQ35_02420"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HET9929507.1"
                     /translation="MATTTSSPTASAGALAPAPNHSVELSGLSARQARPLFDPALVRR
                     AIRASFTKLDPRHQVRNPVMFVVEVGSVFTTGLFVAALGGKLSESPWFILGVSGWLWI
                     TVLFANFAEAMAEGRGKAQADTLRATRKDVLAKRLSAPRRDATVQPLSANALRKGDHV
                     LVEAGDTIPGDGEVVVGVASVNESAITGESAPVIRESGGDRSAVTGGTVVLSDWLIVR
                     ITTNPGETFLDRMISMVEGAKRQKTPNEIALDVLLAALTIVFLLATATLLPYSLFGVQ
                     AAGHGAPVTITVLVALLVCLIPTTIGGLLSAIGIAGMDRMIQANVIAMSGRAVEAAGD
                     VDVLLLDKTGTITLGNRQATAFAPAPGVSAAALADAAQLASLADETPEGRSIVVLAKD
                     RYGIRERDVHALGATFVPFTAQTRMSGVDLRERQIRKGAADTIEAYVKSHGGALPREV
                     RNSVESIAKQGGTPLVVAEGARALGVIQLKDIVKGGIKERFLELRRMGIKTIMITGDN
                     PLTAASIAAESGVDDFLAEATPEAKLALIREHQTGGRLVAMTGDGTNDAPALAQADVA
                     VAMNSGTQAAKEAGNMVDLDSNPTKLIEIVQIGKQLLITRGALTTFSIANDVAKYFAI
                     IPAAFATTYPVLDRLNVMRLHSPASAILSAVIFNALIIVCLVPLALRGIRYKAREAAA
                     LLRHNLLVYGVGGLLLPFVGIKLIDVLLVFMHLA"
     gene            4471..5040
                     /gene="kdpC"
                     /locus_tag="VFQ35_02425"
     CDS             4471..5040
                     /gene="kdpC"
                     /locus_tag="VFQ35_02425"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HET9929508.1"
                     /translation="MISQIRSALAMLLSLSALTGLGYPLLVTGVARAAFPRQASGSLL
                     IEAGRAKGSRLIGQPFDAPRYFWSRPSATTPAYNAAASSGSNLGPSNPALADAVKARV
                     KALREADPGNDDPVPVDLVTASGSGLDPHLSPAAALYQVRRVARARALDEAKVRTLVQ
                     NHVEKRVLGVLGEPVVNVLELNLALDELD"
     gene            5146..7866
                     /locus_tag="VFQ35_02430"
     CDS             5146..7866
                     /locus_tag="VFQ35_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HET9929509.1"
                     /translation="MQDSRPNPDDLLRRVVDEEKRRSRGKHTIFFGAAPGVGKTYTML
                     EVARSELEREKRDVVIGVVETHGRYETGALVLGMELLPRRTSSYRGIELSEFDLDAAL
                     ARRPELILMDELAHTNAEGGRHPKRWQDVDELLDAGIDVFSTMNVQHLESLNDVIAKI
                     TGVHVRETVPDSVLEAAHDIKLVDLPPDDLLERLREGKVYVPAQAQRAIENFFRKGNL
                     IALRELALRHTAERVDAQMRAYRSAQGIEQTWAATEQLVVAVSPSPYSARLIRAARRM
                     ASALHARWFAVYVEPERVARLPKAAQQRLSENLRLATQLGAEVVTLLGNDASVEILRF
                     ARERNITKIIVGKPVAHGALQWLRGSLVDRLVRESGDIDVYVTAGDVEAEPTTRDAPT
                     LPKPRASGMAAAVVTVVLATGVSWALFGRQQLADVVMIYLLGIVLVSVRTGLTTSLFA
                     ALLSVAAFDFIFIPPYFTFAIADFRHAVTFVVMFLVAVLLSGLTQRVRHQAARARERE
                     QRTLALYALTRELAGAQDSAAVTEITARHLEQAFQCRVSVFTPGASGKLERNYASKGS
                     EGELDRDASISQWVFYNKQEAGLGTRTLPSSSTLYVPLMASGSLVGVLGLTPEAPDRF
                     DSVEQRRQVDALAVQMALALERARLADETERARREVEAEQLRSALLSSVSHDLRTPLA
                     VIRGAADALLDARTPYEGTTRIELTRTILDEALRLTRLIKNLLDMTRLQAGAMSVKKE
                     WVPLEELIGGALERLSAAIGKREIQVQLPPELLLVHCDASLLEQALINLLENALKYSE
                     GAIEVSASQQEGEVLAAIADRGPGIPVGAETQVFEKFHRAGREGSPGGVGLGLAICRG
                     IIAAHGGRIWVQNREGGGAVFKFTLPNDARAPEVEPEGTSAIAAEPQTPQ"
     gene            7863..>8411
                     /locus_tag="VFQ35_02435"
     CDS             7863..>8411
                     /locus_tag="VFQ35_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929510.1"
                     /translation="MSSPLILTIEDEPPMRKLLRVSLENNGYRWLGADTGKEGLALAA
                     SQSPDLVLLDLGLPDLDGLSVTERLREWSRVPIIVISARGKEDDKVKALDAGADDYLT
                     KPFGVNELLARIRVALRHASSAGRSTPVVELGALKLDLERREVTVRGAEVHLTPLEYK
                     LLAFLAQHAGKVVTHRQILNAVW"
BASE COUNT         1447 a         2777 c         2728 g         1459 t
ORIGIN      
        1 tgaacgcgag cgtcgcctcg gcgccgaaga cctcgtggtg agccacggcg agcgcaacgc
       61 gagcgaagct tgcggcggtg agcaagagcg cgatcgggac gagatcgaaa tcgtcgagag
      121 gcggcggcgc gagagtccga gctccgagcg gacgagggaa gcgaatgacc tgggccattc
      181 acccaaggtg ctcccgcgaa agtcagcccg acgtaaacat gtgccctgtg tgagtcaaga
      241 aagagtcagc gcagggcccc taccgcggcg cggcgtcgct tcttgatcat ttcttgatac
      301 gccgaggcgg agtctttaca tcgcgcgagc gcgcccggcg atagatccta gaggtacgga
      361 ttcaccatgg acgccgttta catcctgttg gtgttcgcgt tgttttcatc tgcgctcggc
      421 ctgattcgca tttgcgaacg cgtctgagtg cccgcggaga aacatgtcta cgctctacgc
      481 cttggccgga atcgttgcgc tcgggctctt ggtctacttg agcatcgcgc tcctcaagcc
      541 ggagacgttc tcatgacgac gaacgacctc ctgcaaatcg ggatcttctt cgccgtgctg
      601 ctggcgctcg ctttgcctct cgggctctac atggcgcgcg tctacgaagg gcggcgcttg
      661 gcgctcgaga aggtctttgg gcccgtcgag cggctcatct atcgcctttc gggggtgaag
      721 cccgactccg agatggactg gaagacgtac gcagtcgcga tgctcgcctt caacatggtc
      781 ggcctgctcg cggtgtatgc gctccagcgc gtgcagggcg cactgccgtt gaacccgcag
      841 gggctccccg gtacgacacc ggacctcgcg ttcaacaccg ccacgagctt tgccacgaac
      901 acgaactggc aaagctacgg cggagaatcg acgctcagct acctcacgca gatgctgggc
      961 ctcaccgtcc agaacttcgt ctcggcggcg tcgggtatgg cggtattggt agccctgatt
     1021 cgcggcttca cgcggcgcga atcaacgacc atcggcaact tctgggtcga cctcgtgcgc
     1081 ggcaatctct acatcttgct tccgctctcg gtcgtaacgg cgctgctctt ggtttcgcag
     1141 ggcgtagtac aaacgttcgc accttatcgc gaggtgccat tgctgcaggc tctccaggga
     1201 gaggcgggaa aggcgccgct gactcagctc atcgcgctcg gacccgccgc ctcccaaatc
     1261 gcgatcaagc agctcgggac gaacggcggc ggcttcttca acgtgaactc ggctcatcct
     1321 ttcgagaacc cgaccgcctt ctcgaacctc gtcgaggtgg tcgcgatctt gctcgtcgcg
     1381 gtcgcgctct gtttcacgtt cggaaagatg gtgaaggaca tgcggcaggg ctgggccctg
     1441 ctcgcggcga tgatgctgat tttcgttccc ctcctcctcc tgtgcgtcgg cgccgaacaa
     1501 gccggcaacc cagcgctccg cgccctcggc gtcgatcagc tggcgagcgc cctgcatgcc
     1561 ggcggcaaca tggaaggcaa ggaggtgcgc ttcggcatcg cgaactcggg gctctggacg
     1621 gcggcaacca cggccgcatc gaacggttcg gtcaacgcca tgcacgattc gctcactccg
     1681 ctcggcggtc tcgctccgct ctggttgata cagctcgggg aaatcgtctt cgggggagtc
     1741 ggttcgggtc tgtatggaat gctcgtcttc gcgatcgtcg cggtgttcgt cgccgggttg
     1801 atggtgggcc gcacgccgga atacctcggc aagaaaatcg aggtctacga gatgaagatg
     1861 gcagcgctcg tcattttgct gccggccacc atcgtgttgc tattcaccgc catcggcgtg
     1921 tccaccgccg cgggcaaagc cggtattgcg aaccccggcg cgcacggctt cagcgaagtg
     1981 ctctacgcct tctcttcggc cgcgaacaac aacggaagcg ccttcgccgg gctcgccgcg
     2041 aacacaccgt tttacaacac gctgctcggc gtcgcgatgt tcgcggggcg ctactacatc
     2101 aagatcgcgg tcctcgccct cgccggcgcc ctcgcccaga agaggctcgt ccccgcgagc
     2161 agcggcactc tcccgacgca tacgccgctc ttcgtcggtt ggctcaccgc aaccatcgtg
     2221 atcgtcggcg cgctcacttt cattcctgcg ctcgcgctcg gcccagtcgt cgagcacctg
     2281 cggctgcatt catgagcttt cccatggcaa ccacgacgtc atccccaacc gcctcggccg
     2341 gcgcgctcgc tcctgcgcca aaccactccg tcgagctctc cgggctgagc gctcggcaag
     2401 ctcgcccact gttcgatcca gcgctcgtga gacgcgcaat ccgagcctcc tttacgaagc
     2461 tcgatccgcg gcatcaggtg cgtaaccccg tgatgttcgt ggtggaggtc ggcagcgtgt
     2521 tcacgaccgg cctcttcgtt gcggccctcg gcggaaagct gtccgagagc ccttggttca
     2581 tcctcggggt gagcggctgg ctgtggatca cggtgctgtt cgcgaacttc gccgaagcca
     2641 tggcggaggg gcgcggcaag gctcaagccg atacgctccg ggcgacacgc aaggacgtgc
     2701 tcgccaagcg actctccgcg ccgcgtcggg acgcgacggt ccagccgctg tcggccaatg
     2761 ccctccgcaa gggtgaccat gtactggtcg aggcgggtga caccattccc ggggacggag
     2821 aggtggtcgt tggcgtcgcg tcggtgaacg agagcgccat caccggagaa agcgcgccgg
     2881 tgatccggga gagcggcggg gaccgcagcg ccgtgaccgg ggggaccgtc gtcctctccg
     2941 attggctcat cgtgcgaatc acgaccaacc ccggcgagac cttcctcgac cgtatgattt
     3001 cgatggtcga gggcgcgaaa cgccagaaga cgccgaacga gatagcgctc gacgtcctac
     3061 ttgcggcgct gaccatagtg ttcttgctcg cgacggcgac gctgctccct tactccctct
     3121 tcggcgtgca ggcggcgggg cacggcgcac cggtgacgat aacggtgcta gtcgcgttgc
     3181 tcgtctgcct cattccgacc acgatcggcg gtcttctctc ggcaatcggc atcgcgggca
     3241 tggatcggat gatccaagcc aacgtcatcg cgatgtcggg gcgcgccgtc gaagcagcgg
     3301 gggacgtcga cgtcctcttg ctcgacaaga ccggaacgat caccctcggc aaccggcaag
     3361 ctaccgcgtt cgcgccggcg cccggtgtgt cagcggccgc gctcgccgat gctgcgcagc
     3421 tcgcgtccct cgccgacgag acccccgagg ggcgcagcat cgtcgtcctc gcgaaggatc
     3481 gctacggcat tcgcgagcgg gacgtgcacg cgctcggcgc gaccttcgtg ccgttcacgg
     3541 cgcagacgag gatgagcggc gtcgacctgc gagaacgtca gattcgcaag ggagccgccg
     3601 ataccatcga agcttacgtg aagtctcacg gcggcgcgtt gccacgcgag gtacgaaact
     3661 cggtcgagag catcgcgaag caaggcggta cgccgctggt ggtcgccgaa ggagcccgcg
     3721 cactcggggt gattcagctg aaagacatcg taaaaggcgg catcaaggag cgcttcctcg
     3781 agctccgccg catgggcatc aagaccatca tgattacggg cgacaacccg ctcacggccg
     3841 cgtcgatcgc ggcggagtcg ggcgtcgacg actttctcgc cgaagcgact cccgaagcga
     3901 agctcgcgct gatacgcgag caccaaaccg gcgggcggct cgtcgcgatg acgggtgacg
     3961 ggaccaacga cgctccggcg ctcgcccaag ccgacgtcgc cgtggccatg aacagcggca
     4021 ctcaagccgc caaagaagcg ggcaacatgg tcgacctcga ctcgaacccg accaagctca
     4081 tcgagatcgt gcagattggc aagcaactct tgatcacacg cggcgcgctc accacgttca
     4141 gcatcgcgaa cgacgtcgcc aagtacttcg cgatcatccc cgcggcgttc gcgacgacgt
     4201 atcccgtgct cgaccgcctg aacgtgatgc gcctgcactc gcccgcgagc gcgatcttgt
     4261 cggcggtgat cttcaacgcc ctcatcatag tatgtctggt gccgctcgca ttgcgcggca
     4321 tccgctacaa ggcgcgagaa gcggccgcgt tgctgcgaca caatctgctc gtctacgggg
     4381 tgggcgggct gctcttgcca ttcgttggca tcaagctcat cgacgtgctc ttggtgttca
     4441 tgcacctcgc gtgagcccga ggatccgacc atgatttcac aaattcgttc cgccctcgcc
     4501 atgctgctct cgctcagtgc gctcactggg ctcggctacc cgctgctcgt caccggcgtc
     4561 gctcgagccg cgttcccgcg gcaagcgagc ggaagcttgc tgatcgaagc cggccgagcc
     4621 aagggctcgc gcctcatcgg gcaaccattc gacgcgcccc gctatttctg gagccgtccg
     4681 tcggcgacga cgcccgccta caacgcggcc gcttcgagcg gctcgaacct aggcccttcg
     4741 aacccggcgc tggccgacgc cgtcaaggct cgcgtcaaag cgttgcgtga agccgatcca
     4801 ggcaacgacg accccgtccc ggtggacttg gtgaccgcct ctggcagcgg actcgaccct
     4861 cacctctctc cggctgcggc gctctatcag gtccgccgcg tcgccagggc gcgggcgctc
     4921 gacgaggcca aagtgcgcac actcgtgcaa aaccacgtcg aaaagcgcgt tttgggcgtg
     4981 ctcggagagc cggtggtcaa cgtgctcgag ctcaacctcg cgctcgacga actcgactga
     5041 gaatgcgcgc cgactcttcg atgccgtcgt ccatccgagt ggcttcggaa gggctcaaaa
     5101 agtactcgca aggacgctcg ttccgagatc tgatgcgcag gtagcatgca ggattcgcga
     5161 cccaaccccg atgatctcct gcggcgcgtc gtggacgaag aaaagcgtcg ttcgcgcggc
     5221 aagcacacga tcttcttcgg cgctgcgccc ggggtgggca agacctacac gatgctcgaa
     5281 gtcgcgcgct cggagctcga gcgcgaaaag cgcgacgtcg tcatcggcgt ggtcgagacg
     5341 cacgggcgct acgaaaccgg agcgctcgtg ctgggcatgg agctcttgcc gcggcggacg
     5401 tcgagctacc gcggcatcga gctctcggag ttcgatctcg acgcggcgct cgcgcgcagg
     5461 cccgagctca tcttgatgga tgaactcgcg cacacgaacg ccgagggcgg ccgccatccg
     5521 aagcgctggc aagacgtcga cgagttgctc gacgccggca tcgacgtgtt ctcgacgatg
     5581 aacgtgcagc acctcgagag tttgaacgac gtcatcgcga agatcacggg cgtgcacgtg
     5641 cgcgaaacgg tgccggactc ggtgctcgaa gcggcgcacg acatcaagct cgtggatttg
     5701 ccgccggacg acctgctgga gcgtctacgc gaagggaaag tgtacgtgcc ggctcaagcc
     5761 cagcgagcca tcgagaactt ctttcgtaag ggcaacctca tcgccctccg cgagctcgcg
     5821 cttcggcaca cggccgagcg ggtagacgcg cagatgcgcg cctatcgcag cgcccaaggc
     5881 atcgaacaga cgtgggccgc gacggagcag ctcgtggtcg cggtgagtcc gagcccgtac
     5941 tcggcgcgtc taatccgcgc ggcgcggcgc atggcctcgg cgttgcacgc gcgttggttc
     6001 gccgtctacg tcgagcccga gcgcgtggcg cgcctgccca aggccgcgca gcaaaggctc
     6061 tcggagaacc tgcgcctcgc gacgcaactc ggcgcggagg tcgtgacgct gctcgggaac
     6121 gacgcgagcg tagaaatcct tcgcttcgcg cgcgaacgca acatcacgaa gatcatcgtc
     6181 ggtaagcccg tcgctcatgg cgcgctgcaa tggctccgcg gctccttggt ggatcggttg
     6241 gtgcgggaga gcggtgatat cgacgtgtac gtgaccgcgg gcgacgtcga agccgaacca
     6301 acgacgcgtg acgcgccgac gttacccaag ccgcgcgcgt cggggatggc cgcggccgtg
     6361 gtcacggtcg tgctcgcgac gggcgtatcg tgggcgctgt tcggccggca gcagctcgcg
     6421 gacgtggtga tgatctatct gctcggcatc gtgctcgttt cggtgcgcac cgggctcacg
     6481 acctcactgt tcgctgccct gctcagcgtg gccgccttcg acttcatctt cattccgcct
     6541 tacttcacct tcgccatcgc cgactttcgc cacgccgtga cgttcgtggt gatgttcctg
     6601 gtggcggtgc tactgagcgg tctcacgcaa cgcgtacgcc accaagccgc gcgcgcgcgc
     6661 gaacgggagc agcgcacgct ggccctttac gctttgactc gcgaactcgc gggcgcgcag
     6721 gacagcgccg ccgtcaccga aatcacggcg cgtcacctcg agcaggcgtt tcagtgtcga
     6781 gtgagcgtgt tcacgcccgg ggcgagcgga aagctcgaac gaaactacgc ctcgaaaggc
     6841 tccgagggag agctcgaccg cgacgcgagc atcagccagt gggtatttta caacaagcag
     6901 gaggccgggc tcggaacgcg gactctgccg agttcgagca cgctgtacgt gccgttgatg
     6961 gcatccggca gcttggtggg agtcttgggg ctcacgccgg aggcgccaga tcggttcgat
     7021 tcggtcgaac agcgccgcca agtcgacgcg ctcgcggtcc agatggccct cgcgctcgag
     7081 cgcgctcggc tcgccgacga gaccgagaga gcgcgacgcg aggtcgaggc cgagcagctt
     7141 cgcagcgcgc tcttgagctc ggtgtcccac gatctacgaa ccccgctcgc ggtgattcgg
     7201 ggcgcggccg acgctctctt ggatgcgcgg acgccatacg aggggacgac tcgtatcgag
     7261 ctcactcgca cgatcctcga cgaagcgctg cgcttgaccc ggctcatcaa gaatctgctc
     7321 gacatgactc gcctgcaagc cggggccatg agcgtcaaga aggagtgggt gcccctcgag
     7381 gagctgatcg gtggcgcgct cgagcggctg agcgccgcga tcggcaagcg ggaaattcag
     7441 gtgcagctcc ctccggagct tctgctggtt cactgcgatg cgagcctgct cgagcaagcg
     7501 ctcatcaatc tgctcgagaa cgcgctcaag tacagcgagg gcgccatcga ggtgagcgcc
     7561 tcgcaacagg agggcgaggt gctcgccgcc attgcggacc ggggcccggg gatcccggtc
     7621 ggcgcagaaa cccaggtttt cgagaagttt catcgcgccg ggcgcgaggg ctctcccggc
     7681 ggtgtcggcc tcgggctcgc catctgtcga ggcatcattg cggcgcacgg cgggcgaatt
     7741 tgggttcaga atcgagaggg cggcggcgcc gtcttcaagt tcacgctacc gaacgacgct
     7801 cgggcgcccg aagtcgaacc ggaaggcacc tcggcgatcg ccgccgagcc ccagactccg
     7861 caatgagctc acccttaatc ctgacgatcg aagacgagcc gccgatgcgc aagctgttgc
     7921 gtgtctcgct cgagaacaac ggctaccgat ggctcggcgc ggacaccggc aaagaggggc
     7981 tcgcgctcgc cgcctcgcag agccccgatt tggtattgct cgatttgggc ttgccggatc
     8041 tcgacgggct ctccgtgacc gagcgactgc gtgagtggag ccgcgtaccc atcatcgtga
     8101 tctcggcgcg cggcaaagaa gacgacaagg tgaaggcgct cgacgcgggt gccgacgact
     8161 acctcacgaa gccattcggc gtgaacgagc tcctagcgcg catccgggta gcgctgcgtc
     8221 acgcgagctc cgccgggcgt tcgactccgg tggtcgagct cggagccctg aagctcgacc
     8281 tcgagcgccg cgaagtcacg gtgcgcggcg ccgaggtgca cctcacgccg ctcgagtaca
     8341 agctgctcgc atttctggcc cagcacgccg gcaaggtcgt gacccatcgg cagattttga
     8401 acgcggtgtg g
//
LOCUS       DASTKS010000114         5423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1579625, whole genome shotgun sequence.
ACCESSION   DASTKS010000114 DASTKS010000000
VERSION     DASTKS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579625"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFQ35_02440"
     CDS             complement(<1..958)
                     /locus_tag="VFQ35_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9929511.1"
                     /translation="MPPEPPQSPARASGSSDGLLFDWNGTERPPLTPGVVDETLRDGL
                     QSPSAKDPPIADKLELLERAASLGIDCFALGFPASHRRQFDDALRLAQEVAASRLPIE
                     VCCAARTLESDITPIIELSQRTGIRLLVGMFIGSSPIRRATEGWTLEQTLKLSERALE
                     LAISNGLEVLFVAEDSTRSEPDTLKALYETAVRSGAQRVCVADTVGHSTPEGAARIVG
                     FVRELVHALDPRVAVEWHGHRDRGLDVANCLAAWRAGAARCHGTALGIGERVGNAPIE
                     LLLVNLALLGWRELDLRALPAYVRVAARALGFEVGPQQPVVGD"
     gene            complement(988..1842)
                     /locus_tag="VFQ35_02445"
     CDS             complement(988..1842)
                     /locus_tag="VFQ35_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9929512.1"
                     /translation="MRFSHDGAFLRRLAHAGARFGPRPLLKYSPPAFGVLSALMLPEK
                     RRRVAETLSWVHGEGWESSQREVFTTFMEYARCLAEALASGRPEAKDATCHVQGEAFL
                     RDVLEQGRGVIVGTAHTGPWDAAAARLKQAFSRDVVVVMREEPDAAARALHDRVRAEA
                     GVRVVHVGSHPLDAMPLVRELRRGSIVAFQLDRSPPNSRELEVTLFGRRASLPEGPFR
                     LAALANAPILPLFARRRAFFEYDLVVYRPIELSRDASRAELERAAQAAADAMQSFIRE
                     RPTQWFNF"
     gene            complement(1903..2796)
                     /gene="xerD"
                     /locus_tag="VFQ35_02450"
     CDS             complement(1903..2796)
                     /gene="xerD"
                     /locus_tag="VFQ35_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET9929513.1"
                     /translation="MALESLVDAYLSHLRVERALSPHTLAAYGRDLSKFVAFAEEQGV
                     SEPRELNLGVVSAWLGSLAKAGLGPRSAARHLSAARGLMRFLVRENELNDDPTRLAAR
                     PRIGRRLPHPLSEDEMLSLIEAPDPSTLRGLRDRAMLSVTYAAGLRVSELVGLKLGDV
                     DLERGIVSAFGKGQKRRLVPLGEVALQHLEAYLTARAAEEKRRAPSEVLFRSPRGGAL
                     TRQGFWKIVRQHALAAGLRNRVHPHQLRHSFATHLLRGGADLRSVQTLLGHANIATTE
                     VYTHVSRDHVRLAHSRAHPRA"
     gene            complement(2841..3614)
                     /locus_tag="VFQ35_02455"
     CDS             complement(2841..3614)
                     /locus_tag="VFQ35_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HET9929514.1"
                     /translation="MAVLESILAAKRSFVRELAGRPLPAPPPLRPFSLKRPPGGELGL
                     IAEIKRRSPSAGALSTKLSVEERAAAYEGAGARMVSVLTDTPFFDGAYEHLTRARAAC
                     KLPLLCKDFVIDERQLDAARAYGAEAVLLIVRCLDTASLSRLHRAALERELVPFVEVA
                     NEEETRVAIDVGATLVGVNARDLDTLQMDRERAARVLELLPTNITRVHLSGLGSAAAV
                     GAIAASAVDAALIGEALMRADDPAPLLSAMIAEARRHAA"
     gene            complement(3620..4663)
                     /gene="trpD"
                     /locus_tag="VFQ35_02460"
     CDS             complement(3620..4663)
                     /gene="trpD"
                     /locus_tag="VFQ35_02460"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9929515.1"
                     /translation="MSDSVTFAEVFSELGAKRSLSPERVHQVFAAIFEGRWTAAQIAG
                     FLVALRLVGESAEIIAAAASAMRQAMLRVEHSFPLVLDTCGTGGDHSRTLNLSTGAAV
                     IAAASGVPVAKHGNRAATSKSGSADVLEALGIPVDVAAASQKDVLTRANITFLFAQAH
                     HPSMRHAMPVRRELGIRTLFNCLGPLANPAGATHQLLGAFDHDIRPVLAGALKALGAR
                     RAWVVRSVDGMDEISPYAPTRVTALDGGSIEEFEIAPEDFGITPSAPGAVDGGEPKDN
                     ARVLEAVLRGEPHPSSDAFVLNAAAALVVAEGLAPRAATDKARELIASGAALRTLNTW
                     REAVAAVRATQGG"
     gene            complement(4660..5244)
                     /locus_tag="VFQ35_02465"
     CDS             complement(4660..5244)
                     /locus_tag="VFQ35_02465"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET9929516.1"
                     /translation="MNRTIVVVDNYDSFTYNLVQYVASLGANCQVRLNDRVTTDEIRS
                     MAPHGILLSPGPGTPDDAGVTLDVIRELSSETPVFGVCLGLQSIGQAFGGKVIRAPRL
                     MHGKTSPIEHDGRGVFTGVPSPFQATRYHSLVVEEATLPACLEVSARTPEGEIMGIRH
                     RTLPVEGVQFHPESILTEHGLDLVQNWLNSLGKS"
BASE COUNT          911 a         1769 c         1878 g          865 t
ORIGIN      
        1 catcgcccac gaccggctgc tgcggaccga cttcgaaccc gagcgccctc gccgcgaccc
       61 gcacgtacgc gggtagcgca cgcaaatcga gctcgcgcca gccgagcaag gcgaggttca
      121 cgagcagaag ctcgatgggc gcgttgccca cgcgttcacc gatgccgagc gcggtcccgt
      181 gacaacgcgc agccccggcg cgccatgccg cgagacagtt cgcgacatcg agcccccgat
      241 cgcggtgacc gtgccactcg accgccacgc gcggatcgag ggcgtgcacc agctcgcgta
      301 cgaacccgac gatgcgcgcg gcgccctccg gagtcgagtg cccaaccgtg tcggctacgc
      361 aaacgcgctg cgcgccgctt cgtaccgccg tttcgtacag agccttcagc gtatccggct
      421 ccgaacgcgt gctgtcttcg gccacgaaga gcacctcgag cccattgctg atcgcaagct
      481 cgagtgcgcg ctcgctgagc ttcaacgtct gctcgagggt ccacccttcg gtggcgcgtc
      541 ggatcggact gctaccgatg aacatgccga ctaggagccg aatgcccgta cgttgggaga
      601 gctcgatgat gggagtgatg tcgctctcga gggtgcgcgc tgcgcagcac acctcgatgg
      661 gtaaccggct cgcggcgacc tcttgagcga ggcgcagcgc gtcgtcgaac tgccgacggt
      721 gcgatgcagg aaagccgagc gcaaagcagt cgatgccgag cgacgcggcg cgctccaaaa
      781 gctcgagctt gtcggcgatg ggagggtcct tcgccgacgg cgattggagc ccgtcccgca
      841 acgtctcgtc gacgacgccc ggcgtcagag gcgggcgctc ggtgccgttc caatcgaaga
      901 gcaggccatc ggacgagccg gaggctcgag cggggctctg ggggggctcg ggcggcattc
      961 ggcgactcgg atgcaaatag cgtggaatca gaagttgaac cactgggtgg gccgctcgcg
     1021 gatgaagctc tgcatcgcgt ccgccgcggc ttgagcggct cgctcgagct cggcgcgtga
     1081 agcgtcgcgc gagagctcga tgggccgata caccaccaaa tcgtactcga agaacgcgcg
     1141 gcgtcgcgca aacaagggca aaatcggagc gttcgccagc gccgccaagc gaaacggacc
     1201 ttcgggaagg ctcgcgcggc ggccaaacag cgtcacctcg agctcgcgac tgttcggcgg
     1261 gctgcgatct agctgaaacg cgacgatgct gccccggcgg agctcacgca cgagcggcat
     1321 tgcgtcgagc ggatgacttc cgacgtgaac cacgcgcacg cccgcttctg cgcgcacgcg
     1381 gtcgtgtaag gcgcgcgcgg cggcgtctgg ctcctcgcgc atcaccacca ccacgtcgcg
     1441 tgaaaacgcc tgcttgaggc gcgctgcggc tgcatcccat ggcccggtat gcgctgttcc
     1501 cacgatcacg ccacgacctt gctcgaggac gtcgcgcaag aacgcctcgc cctgaacgtg
     1561 gcatgtggcg tctttggcct cgggccgtcc gctcgccagc gcttctgcca ggcagcgcgc
     1621 gtattccatg aacgtggtga agacctcgcg ctgcgagctc tcccatcctt cgccgtgaac
     1681 ccagctcagg gtttcggcca cgcggcgtcg cttttccggc agcatcagag ccgaaagcac
     1741 gccaaacgcg ggagggctgt atttcagcaa gggcctcggc ccaaaccgcg cgcccgcgtg
     1801 cgctagccgt cgaaggaacg cgccgtcatg agaaaaacgc atcgaagcca gtcacgcttc
     1861 tttcaccatc cgccgccgag agcaagcgcg gcgcaacgcc gatcacgcgc gcggatgagc
     1921 tcgggagtgg gcgaggcgca cgtggtcgcg cgagacgtgc gtgtagacct cggtcgtggc
     1981 gatgtttgcg tggccgagga gtgtttgaac gctgcgcagg tccgcgcccc cgcggaggag
     2041 gtgagtcgcg aaggaatgac gcagctgatg tgggtggacg cggttgcgga ggcccgcggc
     2101 cagcgcgtgc tggcgtacga tcttccaaaa accctggcgg gtgagcgcac cgccgcgcgg
     2161 cgagcgaaac aggacttcgg agggagcgcg gcgcttttcc tcggcggcgc gcgcggtgag
     2221 atacgcttcg agatgctgga gcgcgacttc accgagtggc acgaggcggc gcttttgccc
     2281 cttgccgaag gccgagacga tgccgcgctc gagatcgacg tctccgagct tcaagccgac
     2341 gagctcgctg acgcgcaggc ctgcggcgta agtgacgctc agcatggcgc ggtcgcgcag
     2401 gccgcgcagc gtgctcgggt cgggtgcctc gatgaggctc agcatctcgt cttcactcaa
     2461 cggatgaggc aggcgtcgcc cgatgcgagg gcgcgccgcg aggcgggtag ggtcgtcgtt
     2521 gagctcattt tcccgtacca ggaaccgcat gagtccgcgc gcagccgaga ggtgccgcgc
     2581 ggcgctgcgc ggcccgagcc cagccttcgc gagcgagccg agccacgcgc tcacgacgcc
     2641 gaggttcagc tcgcgcggct cgctcactcc ttgctcctcg gcaaaggcga cgaacttcga
     2701 gagatcgcgc ccgtacgcgg ccagagtgtg gggcgataaa gcccgctcga cacgcaagtg
     2761 actcaggtat gcgtcgacca gactttcgag cgccatctcg ggccactaga gtagctcgag
     2821 agggagcgct cggccgaatt tcaagcggcg tggcgtcgcg cttccgcgat catggccgag
     2881 agcagcggcg ccggatcgtc ggcgcgcatc agggcttcgc cgatgagcgc cgcgtcgacg
     2941 gcgctcgcgg cgatggcgcc gacggcggcg gcggacccga gaccggagag gtgcacccgc
     3001 gtgatgttgg tcggtaggag ctcgaggacg cgcgcggctc gctctcgatc catctgcagg
     3061 gtgtcgagat cgcgcgcgtt cacgccgacg agagtggctc cgacgtcgat ggcgacgcgc
     3121 gtctcttctt cgttcgcgac ttcgacgaac ggcacgagct cccgttcgag cgcggctcgg
     3181 tgaagccggg aaagcgacgc cgtgtcgagg cagcgtacga tgagcagcac cgcctcggcg
     3241 ccgtatgcgc gggccgcatc gagttgtcgc tcgtcgatga cgaagtcttt gcagagcagc
     3301 ggcagcttgc aggcggcgcg ggcgcgggtg aggtgctcgt aggccccgtc gaagaacggc
     3361 gtatcggtga gcacgctgac catgcgcgcg ccggcaccct cgtaagcagc cgcgcgctcc
     3421 tcgacgctga gcttcgtcga gagcgcgccc gccgacggag agcgccgttt gatctcggcg
     3481 atgagcccga gctcgccgcc ggggggacgt ttcagagaga acgggcgcag cggcggcgga
     3541 gcggggaggg gacggcccgc gagttcgcgg acgaaggacc gcttcgcggc aaggatggat
     3601 tcgagcacgg ccacggcgtt cagcctcctt gcgtcgctcg tacggcggca acggcttcgc
     3661 gccaggtgtt caacgtgcgg agcgccgcgc ccgaagcgat gagctcacgc gccttatcgg
     3721 tggcggcgcg cggcgcgagc ccttcggcaa ccacgagcgc ggcggcggcg ttgaggacga
     3781 aggcgtcgct cgaggggtgg ggttcgccgc gcagaacggc ttcgagcacg cgcgcgttgt
     3841 ctttgggttc accgccgtcg acggcgccgg gcgcgctcgg cgtaatgccg aagtcttctg
     3901 gcgcgatctc gaactcttcg atgctcccgc catcgagcgc ggtgacgcgc gtcggggcgt
     3961 aggggctgat ctcgtccatg ccatccacgc tgcgcaccac ccacgcgcga cgcgccccga
     4021 gcgccttcag cgcgccggcg agcacgggtc gaatgtcgtg gtcgaacgct ccgagcagct
     4081 gatgggttgc gccggctggg ttcgcgagcg gtcccaagca attgaacagg gttcgaatcc
     4141 cgagctcgcg gcgcacgggc atcgcgtggc gcatcgaggg atggtgcgcc tgcgcgaaca
     4201 agaaggtgat gttggcgcgc gtgagcacgt ccttttgcga agccgcggcg acgtcgacag
     4261 ggatgccgag cgcttcgagc acatcggcgc tgcccgactt gctcgtggcg gcgcggttgc
     4321 cgtgtttcgc taccggcact ccgctcgcgg cggcgatgac ggccgcgccg gtggagaggt
     4381 tcagggtgcg cgagtgatcg ccgccagtgc cgcacgtgtc gagcacgagc gggaacgaat
     4441 gctccactcg caacatcgct tgtcgcatcg cgctcgccgc ggctgcgatg atctcggcgc
     4501 tctctccgac caagcgcagc gccaccaaga acccggcgat ttgcgccgcc gtccagcggc
     4561 cctcgaagat cgcagcgaag acttggtgca cccgctctgg gctcaagctg cgtttcgcgc
     4621 cgagctccga gaacacctcc gcaaacgtga ccgagtcgct catgactttc ccaaagagtt
     4681 cagccaattc tgcaccaagt cgagcccatg ttcggtgagg atcgattcgg gatgaaactg
     4741 cacgccttca acgggcagcg tgcggtgtcg aatccccatg atttcaccct cgggagtgcg
     4801 ggcactgacc tcgagacacg cgggcaacgt cgcttcctcc actaccaaag aatggtaacg
     4861 agtcgcctga aacgggctcg ggacgccggt gaacacgccg cgtccgtcgt gctcgatggg
     4921 agaggtcttg ccgtgcatca agcgcggggc acgaatgact ttgccgccaa atgcttgacc
     4981 tatcgactgt aggccgaggc acacgccgaa caccggagtt tcgctcgaaa gttcacgaat
     5041 tacgtcgagc gtgacgcccg catcgtcggg ggtgcctggc cccggcgaca gcaagattcc
     5101 atgcggagcc atggacctga tctcgtcggt ggtgacccga tcgttcaagc gcacctggca
     5161 atttgcgccg agcgaggcaa cgtactgcac caagttgtat gtgaatgagt cgtagttatc
     5221 gacgacgacg atagtgcggt tcacgagtgt cccgggttct actacgccgc ggccttccag
     5281 gcacatgcgc gagaaactcg caggatttgc gccttggccg cggcgcgtgg ccgattccgt
     5341 ttcgctctcg ggcgattatt tcgccattgt ggggctcgac atcgttgacg agtctcgctt
     5401 tttcaatctt gcccgcgggt tag
//
LOCUS       DASTKS010000115         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_465191, whole genome shotgun sequence.
ACCESSION   DASTKS010000115 DASTKS010000000
VERSION     DASTKS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465191"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..3320
                     /locus_tag="VFQ35_02470"
     CDS             27..3320
                     /locus_tag="VFQ35_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929517.1"
                     /translation="MPRSVPARPRTSWLFLGGALALGAVVVLCAMAAFGYVTASRWVD
                     HTLSVQSEIDQWVSSLDRAHHAAVVYVTTGSEEALHLYDSSLAQSRREEARVEALVGD
                     NTQQLQRVSRARFAASSVLGRLHAQVVQAQAGHRSEATRELSSGVDAELVSEFRQSAR
                     RIRTEEAQLLNERRAHADSRAWIVLIGAGVLAAVSLALLVFAWRREAAHEQHVSNMAR
                     EARQRLRILSELATALSSARAPDEVGEVVVQHGMQAAGGDTCSLYLLNSSKTELRLIA
                     ARGVSPELVDKLRRVSATEGFSGIFQAVEAGRSIWVQNEREYAATYPDLASLKVDGLR
                     PKAFWSVPMIVEGSTLGLFGVGYYEPRNFSADERAFAETLVQQCAQALLRATRLQAEE
                     AARRWFVTTLRSIGDAVIATDSDGRVMFVNPVAERLTGYAESEAVGKPLTEVFQILSE
                     LTRAPAEDPVAKVLREDRVIGLANHTLLRQRNGAEIPIDDSGAPIRDENGSVVGVVLV
                     FRDVSEKKRAELQNEYLAKAGEALASSLDYRTTLATIAEFAVPKFADWCAVDLVEPGT
                     GTPKQVAVAHVDPNKVAFARQLGERYPPDPNARNGVPEVVRTGESELYVDIPPEMLEA
                     AAQDEEHRRVIRELKLRSAMVVPLRARGRTFGAMTFVYAESGRHYEREDLSFAEDLAR
                     RAAMAIENAMALRDADEARSLERWMRGEAERANRAKDEFLATVSHELRTPLNAILGWA
                     VTLRSRKPPEDIDRALAVIERNARSQAKLIDDVLDVSRIISGKLSLTLTPTNVATALR
                     KAVETVTPAAQAKNISVSVDVSDDNLSILADGERLQQVAWNLLSNAVKFTPKQGHVSV
                     SLKSDGSEIVIRVTDDGEGIRSDVLPLVFEPFQQADASTTRRHGGLGLGLSIVKQLIV
                     AHGGSVSVESDGPGRGATFTVRLPARQSSSAVADAEPSSQDGAAVKGQLDMLAGLRVL
                     IVDDEPDALQLLRAVLTEAGAEVHAAGSASEALQRFLEVRPDVLVSDIGMPNEDGYSL
                     IRKVRAQPVDRGGRTPAVALTAYARPQDVQRAFVAGFQMHVVKPVEPAALRSVVANLG
                     GRSLETSSA"
     gene            3376..>3888
                     /locus_tag="VFQ35_02475"
     CDS             3376..>3888
                     /locus_tag="VFQ35_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9929518.1"
                     /translation="MASAWALSFLGYALYYVGRKGFSVSKKTLVREEGLSEAALGGID
                     SAYLAAYALSQFASGVLGDRWGARRLVTFGLLGSALACACFGSLHTALGFTLAFLANG
                     WFQATGWPGTTRVMADFTTPRNRGTVMAFWSTCYQLGGILANVLCGVLLVRFGWRSAF
                     WVPALVMALMA"
BASE COUNT          636 a         1237 c         1334 g          681 t
ORIGIN      
        1 ggagcggagg tactctggga cggaacatgc ctcgatccgt cccggctcgc ccgcgaacga
       61 gctggctctt tttgggcggc gcgctggccc ttggagcagt cgtcgttctc tgcgcgatgg
      121 ccgcgttcgg gtacgtcacc gcgtcgcgtt gggtggatca caccctgtcg gtacagtccg
      181 agatcgacca atgggtgagc tcgctcgatc gagcgcacca cgccgcggtc gtgtacgtga
      241 ccaccggcag cgaggaggcg ctccacctct acgattcttc ccttgcgcag tcgcggcgcg
      301 aggaggctcg ggtcgaggcg ctggtcggcg acaacacgca gcagctgcag cgggtttcac
      361 gtgcgcgctt cgcggcgagc tcggtgcttg ggcgccttca cgcgcaagtg gtgcaggcgc
      421 aggcggggca ccgtagcgaa gcgactcgtg agctttcgtc gggggtcgac gccgagttag
      481 tgagcgagtt tcggcagagc gcgcgccgta taagaaccga agaagcccag ctcctgaacg
      541 agcgacgtgc tcacgccgac tcgcgtgcgt ggattgtgct catcggcgcg ggcgtcttgg
      601 cggcggtttc actcgcgctg cttgtcttcg cgtggcgacg tgaagccgcg cacgagcagc
      661 acgtatccaa catggcccgg gaggctcggc aacgcctgcg aatcctctcg gagctcgcga
      721 cggcgctgtc gagcgcgcgc gcgcccgacg aggtgggcga agtcgtcgtg caacatggca
      781 tgcaagccgc tggcggcgac acgtgcagcc tctatctcct gaactcttcg aagaccgagc
      841 ttcgtctcat cgcggcgcgc ggcgtgagcc cggagctcgt ggacaagctt cgccgcgtga
      901 gcgctaccga ggggttctcg ggcatctttc aggccgtcga agccgggcgc tcgatctggg
      961 tgcaaaacga gcgcgagtac gccgccacct atccggatct cgcgagcctg aaggtcgatg
     1021 gcttgagacc caaggcgttc tggagcgtgc ccatgatcgt cgagggcagc actctgggtc
     1081 tgtttggcgt cggctactac gagccgagaa acttctccgc cgacgagcgc gctttcgccg
     1141 aaaccttggt gcagcagtgc gctcaggcgc tgctgcgggc cacacggctg caggcagaag
     1201 aagcggcgcg gcgttggttc gtgaccacct tgcgcagcat cggtgacgcg gtgatcgcga
     1261 ccgactcaga cggccgcgtc atgttcgtca accccgtcgc cgagcgcctc actgggtacg
     1321 ccgagagtga agcggtcggt aagccgctga cggaagtgtt tcagatattg tccgagctga
     1381 cgcgcgcgcc cgccgaagac cccgtagcca aggtgttgcg ggaagatcga gtgatcgggc
     1441 tcgccaatca cacgctgttg cggcagcgaa acggcgcgga gatccccatc gacgacagcg
     1501 gcgcgcccat tcgcgacgag aacggaagcg tcgtgggcgt cgtgctcgtg tttcgcgacg
     1561 tcagcgagaa gaagcgcgcc gagctccaaa acgagtacct ggccaaggcg ggggaagcgc
     1621 tcgcctcgtc gctcgactac cgcacgactc tcgccaccat cgccgagttc gcggtgccga
     1681 agttcgccga ctggtgcgcg gtcgatctcg tcgagcctgg cacggggaca ccgaagcaag
     1741 ttgcagtcgc ccacgtagac ccgaacaagg tcgcgttcgc gcgccagctc ggcgagcgct
     1801 atccgccaga cccgaacgcg cgcaacgggg ttcccgaggt cgtgcgcacc ggcgagtccg
     1861 agctctacgt cgacatcccg cccgagatgc tcgaggcggc cgcacaagac gaagagcacc
     1921 gacgcgtgat ccgcgagctc aagctgcgtt cggcgatggt ggtgccgctg cgcgcgcgcg
     1981 ggcgcacttt cggcgccatg actttcgttt acgccgagag cggccgccat tacgagcgcg
     2041 aggacctgag cttcgccgag gacctggcac gccgcgccgc catggcgatc gaaaacgcga
     2101 tggcgttgcg tgacgccgac gaggctcgct cgctcgagcg ttggatgcgt ggcgaggcgg
     2161 agcgcgccaa ccgcgccaag gacgagttcc tcgcgacggt gtcgcacgag ctccggacac
     2221 cgctgaacgc gatcctcggg tgggcggtga cactgcgaag tcgcaagcct cccgaggaca
     2281 tcgaccgcgc cctcgccgtc atcgagcgca acgcgcgctc gcaggcgaag ctcatcgacg
     2341 acgtgctcga tgtttcgcgc atcatcagcg gaaagctttc gctgacgctc actccgacca
     2401 acgtcgcgac cgcgctgcgc aaagccgtcg agacggtcac tcctgcggct caagcaaaga
     2461 acatcagcgt ttcggtcgac gtttcggacg acaatttgtc gatcctcgcg gacggcgaac
     2521 gcttgcagca agtcgcctgg aatctgctct cgaacgcagt caaattcact ccaaagcagg
     2581 gtcacgtcag cgtcagcctg aaaagtgatg gatccgagat cgtcattcgg gtgaccgacg
     2641 atggggaagg aattcgcagc gacgtgctac cgctcgtgtt cgagccgttt cagcaagcgg
     2701 acgcttcgac tacccgccgt catggtggcc ttggcctcgg cctttccatc gtgaagcagc
     2761 tcatcgtggc tcacggcggc tccgtcagcg tggagagcga cgggcccggt cgtggcgcga
     2821 cgttcacggt gcgactgccg gcgcggcagt catcgagcgc cgtcgccgac gccgaacctt
     2881 cgagccaaga cggcgccgcc gtaaagggcc aactggacat gctcgctggg ttgcgcgttc
     2941 tcatcgtcga cgacgagccg gatgcgcttc agttgctgag ggccgtgttg accgaagcgg
     3001 gagcggaagt gcacgcggcc ggttcggcga gcgaagcgct gcagcgtttc ctcgaagtgc
     3061 gccccgacgt gctcgtgagc gacatcggta tgccgaacga ggatgggtac tcgttgattc
     3121 gtaaagtacg cgcgcagccg gtcgatcgag gcggtcgcac gccggcggtc gcgctcacgg
     3181 cttatgcacg gccgcaggat gtccagcggg cgttcgtcgc tggctttcaa atgcacgtcg
     3241 tgaagccagt cgagccagcc gcgctccgaa gcgtcgtcgc gaacctcggc ggacgcagct
     3301 tggaaacgtc gtccgcttga ccctctccgc tgcgcgggct ctgatcgccg ggtgtcgacc
     3361 ctcgagcggc cgcgagtggc gagcgcgtgg gcgctgagct tcctcgggta cgcgctctac
     3421 tacgtcggta gaaaaggctt ctcggtctcg aagaagaccc tggttcgtga agaagggctc
     3481 agcgaagccg cgctgggcgg catcgattcg gcgtacctcg ccgcttacgc gctgtcgcag
     3541 ttcgcgagtg gcgtgttggg cgatcgttgg ggcgcgcgcc gcctcgtcac gtttggcctg
     3601 ctcggctcgg cgctcgcgtg tgcctgcttc ggctcgctgc acacggcgct cggattcacg
     3661 ctcgcgttcc tcgcgaacgg ctggtttcag gcgaccggtt ggcccggcac gacgcgagtg
     3721 atggccgatt tcaccacacc gcgaaaccgc gggaccgtga tggccttttg gtcgacctgc
     3781 tatcagctcg gtgggatttt ggcgaacgta ttgtgcggcg ttctattggt ccgcttcggt
     3841 tggcggtctg cgttctgggt cccggcgctg gtgatggcgt tgatggca
//
LOCUS       DASTKS010000116         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1133521, whole genome shotgun sequence.
ACCESSION   DASTKS010000116 DASTKS010000000
VERSION     DASTKS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133521"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1544
                     /locus_tag="VFQ35_02480"
     CDS             294..1544
                     /locus_tag="VFQ35_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET9929519.1"
                     /translation="MSAAGRRSRAPKRAPARRKFLRASLIALATLGGAGAALWGAVHR
                     YDWMGPLVANTLRSIVGVDAVAKLEDFTYAIEDGVNRLTRRHDAPKAYWSVPSNQVDA
                     KAAAPEPEQTEEHAPVPLVRFHPKDPGPALKQWSAPGDGVWVPIRDARYPEEEPFLYK
                     TLLHPDAGRSWSEVFVVALDVRRVDLYVVAGSQEPKLDDPKTVYERKARIPDEHQETV
                     LAAFNGGFMTEHGGYGMKVDGVVLVHPKPTACTLALYKDGSIRIATWSKLASTEPDMV
                     WYRQAPQCMYEGGKIHPGLTAGSGLKWGATLDGDTVIRRSAVGLSQDGQVLYVSITNH
                     TTARALADAMRHAGAVDIAQMDVNWSYPKFVTFEAAAPGEKRKAVALASGFEFSEDEY
                     IRKRSRRDFFYAVRRKSARTLPTP"
     gene            1875..>2392
                     /locus_tag="VFQ35_02485"
     CDS             1875..>2392
                     /locus_tag="VFQ35_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9929520.1"
                     /translation="MIRDLSRRVFLRGLGGACVAAPFLGSLMDRSVKAKSPAAPRRLI
                     VMFTHFGCITTRFFPKRSHGQLTADDLQPTTLKHLVPLVDKLLMPRGIRSMNEWTTDM
                     SRGQGNDPYLQAVGSFLTCQPLTPNSDDPFSFDQATKFRAMPMGPSLDHVMAQQLSPE
                     GTPLVMQVGGLAA"
BASE COUNT          439 a          771 c          784 g          398 t
ORIGIN      
        1 gctcgtccgc ggccgaaagc accttgacca tgcttgccca aggaggctgg ctcgtttcgg
       61 ggagcaatga gacccccggg cccggtgtgt ggggcaaccg gcgacttttg gtacgctcgg
      121 tcaacgtcgc gatatccgtg cgactctcgg ccttgttagc cattcgagcc atgccggtgg
      181 cgactagccc ggctctcggt gctcgagcgg cagtctccga tagcttgcgt catcgccgcc
      241 agggtgcgag aagtcggcgg gaaccagggc gaacaccaag gatgagccag agcatgagtg
      301 cggccgggcg tcgctcgcga gccccaaaac gagcgccggc gcggcgcaag tttttgcgag
      361 cgagtctgat cgcgctcgcc acgctcggtg gtgcaggcgc agccttgtgg ggcgccgtgc
      421 atcgctacga ttggatgggg ccgctcgttg cgaacacatt gcgctcgatc gtcggggtcg
      481 acgccgtagc caagctcgaa gacttcacgt acgcgatcga agatggcgtg aaccgcctga
      541 cgcggcgtca tgacgcgccg aaggcgtact ggtcggtgcc gtcgaaccaa gtggacgcca
      601 aagcggcggc ccccgagccc gagcaaaccg aagagcacgc gcccgtgccg ctcgtgcgct
      661 ttcacccgaa agatccgggc cctgcgctca agcagtggtc ggcgcccggc gacggcgttt
      721 gggtgccgat ccgcgacgct cgttacccgg aggaggaacc ctttctttac aagaccttgc
      781 ttcacccgga tgcggggcgt tcgtggagcg aggttttcgt agtcgcgttg gacgtgcgcc
      841 gggtcgacct gtacgtcgtc gccggtagcc aagagccaaa gctcgacgac ccgaaaaccg
      901 tttacgagcg caaagcgcga atccccgacg agcaccaaga aacggtgctc gccgccttca
      961 acggcgggtt catgaccgag cacggcggct acggcatgaa ggtcgacggc gtcgtgctgg
     1021 tgcacccgaa acccaccgcc tgcacgctcg cgctctacaa agacggctcc atacgcatcg
     1081 cgacctggag caagctcgcg tcgaccgagc ccgacatggt ttggtaccga caagcgccgc
     1141 aatgcatgta cgagggagga aaaatccacc ctggcctgac cgcaggcagc ggcctgaagt
     1201 ggggagcaac gctcgacggc gacacggtga ttcgacgctc cgcggtcggg ctgagccagg
     1261 acggtcaggt cctctacgta tccatcacga accacaccac cgcgcgcgct ctcgcggacg
     1321 cgatgcgcca cgccggcgcg gtcgacatcg cgcaaatgga cgtgaactgg tcgtacccca
     1381 agttcgtgac gttcgaagcg gcggctccgg gggaaaagcg caaggccgtc gcgctcgcga
     1441 gcggcttcga gttctcggag gacgagtaca ttcgtaagcg ttctcggcgc gacttcttct
     1501 acgctgtccg acgcaaatcc gcgcgaacgc tcccgacgcc ttgacgttcg agtctcggtt
     1561 accagcctga acgactcggg ccccagccga cctgaacgaa agtctcctgc gtcacccgaa
     1621 cggccacgct ctccagagcg tacgcgcgaa aagggtgcgc cgcgcaaata caggcacggg
     1681 tcggatgggc atagcccaat ggatagcgag gcttcaatgt gtgcgtgtat gcgggcgcac
     1741 gccggcggca gtggccagcg gcgccaccgt gggtcgtcct ctgggccctt gaattgagca
     1801 atccgcggcg cacgcaaggg cggtgagtgg ctcggacgag cgcgcgccgc gtgtcgaacg
     1861 agtcgaggac gagcatgatc agagatttgt cgcgacgagt attcctgcgc gggcttggcg
     1921 gcgcgtgcgt ggcggctccg tttctcggct ccttgatgga tcgctccgtc aaggcaaagt
     1981 cgccggcagc gccgcgacga ctgatcgtga tgttcactca ttttgggtgc atcacgacgc
     2041 gattcttccc gaagaggtct cacgggcagc tcaccgccga cgacttgcag ccgacgacgc
     2101 tcaaacactt ggtgccgctc gtcgacaagc tcctcatgcc gcgtgggatc cgctccatga
     2161 acgagtggac cactgacatg tcccgcggcc agggcaacga tccctacctg caagcggtcg
     2221 ggtctttcct cacgtgccag cccctcaccc cgaacagcga cgatccgttc agcttcgatc
     2281 aggcaaccaa gttcagggca atgccgatgg gtccctcgtt ggatcacgtg atggcgcagc
     2341 agctgagtcc ggagggcacg ccgctcgtca tgcaggttgg aggcctggcc gc
//
LOCUS       DASTKS010000117         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1133688, whole genome shotgun sequence.
ACCESSION   DASTKS010000117 DASTKS010000000
VERSION     DASTKS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133688"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFQ35_02490"
     CDS             <1..280
                     /locus_tag="VFQ35_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929521.1"
                     /translation="DQQLALGGTYMEYQVVGSAPEGRLEVEVDVADHYFWSPSEKERP
                     THCLHVCGARLVTAGKATEFFQFGEGRLIVDDPLVAAKKAEEEARNGL"
     gene            270..620
                     /locus_tag="VFQ35_02495"
     CDS             270..620
                     /locus_tag="VFQ35_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929522.1"
                     /translation="MASESNSDQEAWSRSALTVALWFAYTPAFLFSPDAAKRAFRALP
                     DHELWALALYAVVSSVCVFLALVVLTVLVRRVAPRTRKKLSLFLLISWLPHTGVVWLL
                     WFLDVIRQPGAWID"
     gene            complement(643..1116)
                     /locus_tag="VFQ35_02500"
     CDS             complement(643..1116)
                     /locus_tag="VFQ35_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929523.1"
                     /translation="MNQPRLYLVCGLPGAGKTTRARKIADSIHALPLCADDWVLGLGQ
                     SMIDYEFRVKLQDCLLAHAATLLRHGVSVVVEFGSWHRAEREAIHHVAAREGATTELH
                     FVNAPPAELAERIRKRGGPEAEALVTVLLEDHEKFEPPSPAEIALFDLYVGPEDE"
     gene            1180..1815
                     /locus_tag="VFQ35_02505"
     CDS             1180..1815
                     /locus_tag="VFQ35_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929524.1"
                     /translation="MAKARTNRTAHVGVAENGNSAVLVTLSAAGELLDRRSVDLTEPG
                     TPTHPHHHEGSWAVGRYLDSPWARKVSLSEAVALVERVREAAERGARERLEELANAVS
                     RPITSIAIRACPALPATVEECIADNRAQSMADSVMYRKALASAAEARGWSLYWYEREQ
                     VSRDAALALGGADLEALLRAMGRSAGPPWQAKHKLAATAAIAAAGSGARRR"
     gene            1992..>2354
                     /locus_tag="VFQ35_02510"
     CDS             1992..>2354
                     /locus_tag="VFQ35_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929525.1"
                     /translation="MRLISWGLWGAMLVSVTVAGCGCDCKSGSILELPKIDASTAGEV
                     RVSWQHDTDAEPRIVCSWQATTSTNSGWSCSPTPLSADDYGTTVRFSYDDTTPSAGWH
                     VAIEAPGGSLENSIPSYST"
BASE COUNT          402 a          697 c          801 g          454 t
ORIGIN      
        1 cgaccagcaa ctcgcgctcg gcggaacgta tatggaatat caggtcgtgg ggagcgcacc
       61 agaaggtcgg ctcgaggtcg aagtcgatgt agccgaccac tatttctgga gcccttcgga
      121 gaaggagcgg ccgacccatt gcctgcacgt gtgcggagct cgcttagtca ccgctggcaa
      181 agcgaccgag ttttttcaat tcggcgaagg ccgattgatc gtcgatgatc ctttggtcgc
      241 agcgaagaag gcggaagaag aggcgcgaaa tggcctctga aagcaatagc gaccaggaag
      301 cttggtcgag atcggctctc accgtggccc tctggttcgc ttacacccct gcgtttctct
      361 tttcacccga cgcagcgaag cgtgcgttca gagctttgcc cgatcatgag ctctgggcgc
      421 ttgcgcttta cgcggtggtt tcgagtgtgt gcgtgttcct cgcgttggtc gtgctcaccg
      481 tgttggttcg gcgggtcgcg ccgcgtacac gcaagaagct gtctctgttc ctcctcatca
      541 gttggttacc ccacaccggg gtggtgtggc tcttgtggtt tctcgacgtc atccgtcagc
      601 cgggagcttg gatcgactag caggcctccg tcactcgggc tgtcactcgt cttcaggacc
      661 gacgtacaga tcgaacagcg cgatctccgc gggggagggc ggctcgaact tctcgtggtc
      721 ttcgagaagc acggtgacta gcgcttctgc ctccgggccg ccgcgcttgc gaattcgctc
      781 ggcgagctcg gccggcggcg cgttcacgaa gtgaagctcg gtagttgctc cttcgcgcgc
      841 ggccacgtga tggatcgctt ctcgctcggc gcggtgccat gagccgaatt cgacgacgac
      901 actgacgcca tgacggagga gcgtggcggc gtgtgcgagt aggcaatcct gtagcttcac
      961 ccggaattca taatcgatca tgctctgccc gagcccgagc acccaatcgt cggcgcagag
     1021 ggggagggcg tgaatcgagt ctgcgatctt tcgcgcgcgc gtggttttgc cggcgccggg
     1081 caacccgcac acgaggtaaa ggcgcggctg gttcattctg cggagtgtga cagctggttt
     1141 cggttgacgc attcgcgcaa ttgcctgaaa aagcgaggcg tggcgaaggc acggaccaat
     1201 cgcaccgctc atgtcggcgt cgccgagaac ggaaattcgg cggtgctcgt cacgctctcc
     1261 gcggctggcg aattgctcga ccgacgcagc gtcgatctga ccgaacccgg aacaccgact
     1321 cacccgcatc accacgaagg atcgtgggcg gtggggcgct atctcgatag cccgtgggcg
     1381 cgaaaggtct cgctgtcgga ggcggttgcg ctcgtcgagc gtgtgcgaga agcggctgag
     1441 cgcggcgcgc gagagagact ggaagagctc gcgaatgcgg tgtcgcggcc catcacttcg
     1501 attgcgattc gcgcctgccc cgcactgccc gcgaccgtcg aagaatgtat cgcggacaac
     1561 cgcgctcaga gcatggcgga ctcggtgatg taccgtaagg cgctcgcgag cgccgctgag
     1621 gcgcgcggct ggtcgctata ttggtacgag cgcgagcagg tatctcgcga tgcagcgctc
     1681 gcgctgggtg gcgccgattt ggaagccctt ctgcgtgcga tgggccgctc ggcggggccg
     1741 ccctggcaag cgaaacacaa gctcgcggcg acggcagcca ttgctgcagc cgggagcggc
     1801 gcgcgtcgca ggtaagtcgg tccgcgaccg agtttggcaa cgctgcgttc ggccccggag
     1861 gttacgtgcc tcgtcccgtt cgaagggtct cggatatgac gcgggtgtca gggggcaata
     1921 cacaatctcg cgcagaatct cggtgactcg cgcaggcgcc agggtggcac gggcattgct
     1981 gagggcgtcg catgcgcttg atttcgtggg gtctctgggg agcgatgttg gtcagcgtca
     2041 cggtcgctgg ctgcgggtgt gactgcaaat cgggcagcat cctcgagttg ccgaagatag
     2101 atgcgagcac cgccggtgag gtccgcgtga gctggcagca cgacaccgat gccgagccgc
     2161 gcatcgtctg ttcctggcag gcgacgacct cgaccaattc cggttggagc tgcagtccaa
     2221 cgccgttgtc ggcagatgac tacggcacga ccgtgcgctt cagttacgac gatacgactc
     2281 cgagcgcggg gtggcacgtc gcgatcgagg ctcccggcgg atcgctcgaa aacagcatcc
     2341 cttcgtattc gacg
//
LOCUS       DASTKS010000118         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1357368, whole genome shotgun sequence.
ACCESSION   DASTKS010000118 DASTKS010000000
VERSION     DASTKS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357368"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFQ35_02515"
     CDS             <1..347
                     /locus_tag="VFQ35_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929526.1"
                     /translation="SAVVGALQSGALSYFSNGCPLIVGLDGVVDPNAAAAVRARTLSD
                     YAAARGVTHFADWQYNRSAFAALSEHSKWTPGALELVTAASPQGPDRFELMSVTWPEG
                     APVASTKPKCAP"
     gene            complement(371..2371)
                     /locus_tag="VFQ35_02520"
     CDS             complement(371..2371)
                     /locus_tag="VFQ35_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929527.1"
                     /translation="MDNRRAVRFCDFVTQPEARIDATLEERSQAPRPWLIALLVCSLT
                     LVAYLPVVEGPFIWDDYQLIFDSPRVQELKPLSAYWGTSFWTRGDIEPSGRNYYRPLA
                     IFSLALDHAVHGENPAGYHVTNALFHGANTLLLFGLLRRRKLAAPQAGLLALGWGLFP
                     RLTEAAAWISGRTDVLAGFFSLLALFLAARDRVWTRWAAAASALLGLFAKEVALAALV
                     GVALSEWFAARGASPPKRVLRIVPAALSGVAYFGTRLWAMGIGIRSNNLSWPERIISG
                     TEALGRYVAMLFDGWRPCIQIGFLGAPSWPHVALGGAVALAGVGFVAHHRRKLDAQSF
                     SALAVFALSLGLVLHIVPITVNIVAADRFMYLPLAALVLLLSPLANRPLRHPACIGLG
                     VLCVSYGVATFHRARTWADEVDFWTTAFREERDHNATSRLELGNVYGRAGLHNYAIAL
                     QMSADTGDYQNFLLASHNAAMRFVVLGRYAEARAILEQLVREMPQIPKFHYALAALCV
                     AEHKFEEADKHLTVAERLYPDSTSVQKLRRAMRELRAEEHAPVPDLSTLNGKLENASR
                     IAVYGRAKEALDLLVAAAASPEITAAQLTDPLLYAFDWGTPRQLTALYERYRAVGGDS
                     PTFAENYRLRMERVARLKALWPSLADNSRAPLPAAEPSARER"
     gene            complement(2411..2992)
                     /locus_tag="VFQ35_02525"
     CDS             complement(2411..2992)
                     /locus_tag="VFQ35_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9929528.1"
                     /translation="MIAKLKRLKRGFTLVELMIVVAIVGILAALAIYGVSKYVKNAKT
                     AEARDALGRMAKDATGAYQREAMSPKVMALGSSSGISHKLCESATKKVPTSVPPAKKY
                     QSKPSDWMETGASAATVGWTCLKFSMDDPQYFQYEYTAVAGGQSTFSCLAHGDLDGDT
                     KASTFYLAGGVKTEGSKKVAVAAPNIGEILPDE"
     gene            complement(3076..3681)
                     /locus_tag="VFQ35_02530"
     CDS             complement(3076..3681)
                     /locus_tag="VFQ35_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9929529.1"
                     /translation="MCAPPPSCTRADRAFAARLCSGKARGFTLIELMIVVVIVGILGT
                     LAVYGTRRYIASSKTGEAIQLIGSIKAGEEAYKDETFSYLDVTGSLKTFYPANSTPGQ
                     AKVMWGGGNADMLAKWNSLGVQPTAPVLFVYACRAGGPDDTVESPTDGGDITIGSWPT
                     AAPGTAWYVVKAKADLDSGGRLSTYVAPSFTNQIFSANEGE"
BASE COUNT          691 a         1247 c         1351 g          720 t
ORIGIN      
        1 gctcggccgt cgttggtgcg ctgcaaagcg gagccctttc gtacttctcc aacggttgtc
       61 ccctgatcgt cggcctcgat ggggtggtcg atccgaacgc agctgccgcc gtccgcgcgc
      121 ggactctttc ggactacgcg gctgcgcggg gggtcacgca ttttgcggat tggcaataca
      181 atcgttccgc attcgcggcg ctttcggagc actcgaagtg gacacctggc gcgctcgagc
      241 tcgtgaccgc tgcgagtccc caaggtcccg accgcttcga gttgatgagc gtcacctggc
      301 cggagggcgc tccagtggcc tcgacgaaac cgaagtgcgc cccgtgaacg tgctcagcga
      361 cgcgtgagcc tcagcgttcg cgcgcgctcg gctcggccgc ggggagcgga gcgcggctgt
      421 tgtcggccag gctcggccag agcgccttca agcgggccac ccgctccatg cggagccggt
      481 agttctctgc gaacgtcggg gaatcgccgc cgacggcgcg atagcgctcg taaagagcgg
      541 tgagctggcg cggcgtgccc cagtcgaagg cgtagagcag cgggtccgtc aactgcgcgg
      601 ccgtgatttc gggtgatgcg gccgccgcga cgagcaagtc gagcgcttcc ttggcgcggc
      661 cgtagacggc gatgcgggat gcgttctcga gctttccgtt cagcgtcgag aggtcaggaa
      721 ccggagcatg ctcctcggcg cggagctcgc gcatggctcg gcggagcttc tgcaccgaag
      781 tcgaatcggg gtacaagcgc tccgcaacgg tcaggtgttt gtcggcttct tcgaacttgt
      841 gttccgcgac gcacagggcc gcgagtgcgt agtgaaactt gggaatctgc ggcatctcgc
      901 gcacgagctg ctcgagtatc gctcgcgcct cggcgtaacg gccgaggacc acgaaacgca
      961 tcgcggcgtt gtgcgaggcg agtaagaagt tttggtagtc gcccgtgtcc gcgctcatct
     1021 ggagcgcgat ggcatagttg tggagcccgg cgcggccata cacgtttccg agttcgagcc
     1081 gcgaagtcgc gttgtggtcg cgctcctccc gaaaggcagt cgtccaaaag tcgacctcgt
     1141 ctgcccaggt gcgcgcgcgg tgaaacgtgg cgactccgta gctgacgcac aagacgccga
     1201 gcccaatgca cgcggggtgt cggagcggcc ggtttgcgag cggcgacagc aacagcacga
     1261 gagccgcgag cggcaggtac atgaaccggt cggcggcgac gatgttgacg gtgatgggaa
     1321 cgatgtggag aactagcccg aggctcagcg cgaagaccgc gagtgcgcta aagctctgcg
     1381 cgtcgagctt gcgtctatgg tgagccacga accccacgcc cgcgagagcg acggcgccgc
     1441 cgagcgcaac gtgcggccaa gaaggcgcgc cgagaaaccc aatctggatg cacggacgcc
     1501 acccgtcgaa cagcatcgcg acgtagcggc cgagcgcttc ggttcccgag atgattcgct
     1561 cgggccagct caagttgttc gagcggatgc cgatgcccat tgcccagagc cgcgtgccaa
     1621 aataggcgac gccgctcaag gcggctggga cgattctcag gactctcttc ggcggtgacg
     1681 cgccgcgcgc cgcgaaccat tcgctgagcg cgacacccac caaagcggcc agcgcgacct
     1741 ccttggcgaa gagcccgagc agagccgacg cggcggcggc ccagcgcgtc cagactcgat
     1801 cgcgggcggc gaggaacaag gcgagcagcg aaaagaagcc ggcgaggacg tcggtgcgcc
     1861 ccgaaatcca cgcggccgct tcggtgaggc gcgggaacaa tccccagccg agcgcgagga
     1921 gccccgcttg cggcgccgcg agctttcgcc ggcgcaagag accgaagagt agcagcgtgt
     1981 ttgccccgtg aaacaacgcg ttcgtgacgt ggtacccggc ggggttctcg ccgtgcacgg
     2041 cgtggtcgag ggcgaggctg aagatcgcta gggggcgata gtaatttctg cccgaaggtt
     2101 cgatgtcgcc gcgcgtccag aacgaggtgc cccagtaggc gctcagcggc ttgagctcct
     2161 gcacgcgcgg agagtcgaag atgagctgat agtcgtccca gatgaagggc ccctcgacga
     2221 cgggtaggta cgcgacgagc gtcaacgagc agaccaagag cgcgatcagc catgggcgcg
     2281 gggcctgcga ccgttcttcc aaagtggcgt cgatgcgagc ttcgggctgc gtcacgaaat
     2341 cacaaaagcg aacagcgcgc cgattgtcca tcgacgcgct gcatttgtta ccgctatgac
     2401 ctgcgtacct tcattcgtcg ggcaggattt cgccgatgtt cggagctgcg accgcgacct
     2461 tcttgcttcc ttccgtcttc acgccgccgg cgaggtagaa cgtggaggcc ttggtgtcac
     2521 cgtcgaggtc accgtgcgcg aggcacgaga acgtgctttg gccgcccgcg acggcagtgt
     2581 actcatattg gaagtactgc ggatcgtcca tcgagaactt caagcaagtc cagccgacgg
     2641 tggccgcaga cgcgcccgtc tccatccagt cgctcggttt cgactggtac ttcttcgctg
     2701 gcgggaccga agtcgggacc ttcttcgtcg ccgactcgca gagcttgtgg ctgatgcccg
     2761 agctgctgcc gagcgccatc accttggggg acatcgcttc gcgttggtat gcccccgtcg
     2821 cgtccttcgc catgcgtccg agcgcgtcgc gcgcctcggc ggtcttcgcg ttcttcacgt
     2881 acttgctgac gccgtagatc gcgagcgccg cgaggatgcc gacgatggcg accacgatca
     2941 tcaactccac gagcgtgaag ccgcgtttca gacgtttgag cttggcaatc atgagtttct
     3001 atttctcctt gaggggcttt cagaggttgt cgggattcgt ttgacggcag atcgaggcga
     3061 gaggacgagg aagtgtcatt cgccttcgtt tgccgagaag atttggttgg tgaagctcgg
     3121 tgcgacgtaa gtgctcagcc ttccgccgga gtccagatcg gccttggcct tcaccacgta
     3181 ccaggctgtt ccgggagcgg cggtcggcca gctgccgatc gtgatgtcgc cgccgtccgt
     3241 cgggctctcg acggtgtcgt cggggccgcc ggcgcggcac gcataaacga agagcaccgg
     3301 cgcggtcggc tgcacgccga ggctgttcca tttggcgagc atgtccgcgt tgccgccgcc
     3361 ccacatgact ttggcttgcc cgggcgtgga gttggcagga taaaaggtct tcagcgagcc
     3421 ggtgacgtcg aggtaactga aggtctcgtc cttgtaggcc tcttcaccag ccttgatgga
     3481 cccgatcagt tggatcgctt caccggtctt cgacgacgcg atgtagcgac gggttccgta
     3541 cacggcgagc gtccccaaaa tgccgacgat gacgacgacg atcatcagct cgatcagcgt
     3601 gaagccacgc gctttgccgc tgcagaggcg cgccgcgaag gcgcggtcag cgcgggtaca
     3661 agaaggtggc ggggcgcaca ctcgcatgct cgtccgcttg cctaagcaac ccgcgtgcct
     3721 ctggccaaat taacggaatt ttcgacgttt ctggcgggtg ccggccagct ccaggggtga
     3781 cactttttgt cactcgaccc aggcagcgag cccgaaaacg tcagtcgcct gggagggtct
     3841 ttgtgctcgc aagtgttcgt cttcagccca gatctcgccg aaaatcgtaa ctccgagcgg
     3901 gctcgcgtga ctcgtgtggg cgcagctgct cgctttcgtc cgagccgtcg ctatgatgcg
     3961 acctcggcat gcgaagcctc acaccgctcg aactttggac cgccgcgtc
//
LOCUS       DASTKS010000119         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_688227, whole genome shotgun sequence.
ACCESSION   DASTKS010000119 DASTKS010000000
VERSION     DASTKS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688227"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1221
                     /gene="pheA"
                     /locus_tag="VFQ35_02535"
     CDS             139..1221
                     /gene="pheA"
                     /locus_tag="VFQ35_02535"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET9929530.1"
                     /translation="MSIDDLRRRIDGIDDELLDLLDRRAHVTREIAQEKWLHGVVAHD
                     PERERKIVERLENALEARADAAFPKSSVAPVFREVMSACLSLEEPQTVAYMGPSGTFS
                     HIAAHSAFGLAARYIETPTIGAVLEAVARGSANYGVAPIENSTEGGVTATLDAFLELD
                     VMIQREFVIEVTQCLLGRERDLGKIRRVYSHPQPLAQCRAWLSRHLPHAELVTSPSTA
                     VAAREAASEEYTAAIGSRLSAQLYSLEIIQESIQDRAENATRFVVVAKSDAERTGRDK
                     TSVAFSAAHVRGALRRALEIFDDEGLNLTRIESRPAAGRRWEYVFFTDFEGHRTDANV
                     AKAIDRLRHACSTVRVLGSYPRNDSP"
     gene            1290..1874
                     /locus_tag="VFQ35_02540"
     CDS             1290..1874
                     /locus_tag="VFQ35_02540"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET9929531.1"
                     /translation="MQLRKLVGYFLRGALVTAPLGLTLYLVLTVLTTLDRVLPVGIPG
                     LGLLITLLLITAIGAFTSNVVGRSVLESTERFMSRVPLVKLVYNSIKDLIGAFVGDRK
                     SFDKPVAVRPQRDSELRLLGFVTRQGLGVLGFPSHVAVYLPQSYNFAGQLVIVPSELV
                     EPIDVASGELMTFIVSGGVSGLGVGQSLLPPPPR"
     gene            complement(1844..2224)
                     /locus_tag="VFQ35_02545"
     CDS             complement(1844..2224)
                     /locus_tag="VFQ35_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET9929532.1"
                     /translation="MSPSEKNIVKSLIAVAWADGRMEASETQVIEGLLSGFDATPEEE
                     EELLEYARTRRTLEDDIPLEELSPEDRELLFSNAALLIHADGSEAASERWVLARLGEI
                     LSLSPQVMAEVLGSVQRGGGGNND"
     gene            complement(2292..>2938)
                     /locus_tag="VFQ35_02550"
     CDS             complement(2292..>2938)
                     /locus_tag="VFQ35_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929533.1"
                     /translation="ALCDEEAVFARIPEIIRRRDARTRELLERDGEPRPAPGVSDELP
                     LFLMALRDVVLARDLAIDAAPGAGEQLDVLAPIFRRVLAFEPKQAARPTLAELRARER
                     GYENVTFVATDASELKLRTELPASADLVLYVRRSEGPNLSLSSLGRLLKCGGRFVVID
                     GEAPAQGDWLSTELEGFERTRVESLDVTRLLGRRDRPAWRVLTGVRTKEEGARR"
BASE COUNT          501 a         1012 c          909 g          516 t
ORIGIN      
        1 gtccgtaagc tgaggaacga gggagactcc tgggccgcag tcgtcgaagg ctcgagcgaa
       61 gtgctcgggt ggaaagacta cggccggatg cacgccgccc tccttctccg aattctgatc
      121 gagcaggcac ggtaaagaat gagcatcgat gacctccgcc gtcgcatcga cggtatcgac
      181 gacgaactcc tggatttgct cgaccggcgc gcccatgtca cgcgcgaaat cgcgcaggaa
      241 aagtggctgc acggggtggt cgcgcacgat cccgagcgcg agcggaaaat cgtcgaacgc
      301 ttggagaacg cgctcgaagc gcgcgccgac gccgcgtttc ctaaaagcag tgtagcgccc
      361 gttttccgtg aggtgatgag cgcgtgtctg tcgctcgaag agccgcaaac ggtggcttac
      421 atgggacctt ccggcacgtt ctcccacatt gcggcgcact cggcgttcgg gctcgccgcg
      481 cgttacatcg agaccccgac gatcggcgcc gtgctcgagg cggtcgcgcg cggctcggcc
      541 aactacggag tcgctccgat cgagaactcc acggagggcg gcgtcaccgc gaccctcgac
      601 gccttcctcg aactcgacgt catgatccaa cgcgagttcg tgatcgaggt cacgcagtgc
      661 ctcctcgggc gcgagcgcga cctcgggaag atccgtcgcg tctactcgca ccctcaacct
      721 ctcgcgcagt gtcgcgcgtg gctctcgcgt cacctgcccc acgccgagct cgtcacttcg
      781 ccgtctactg cggtcgcggc gcgcgaggcg gcgagcgaag agtacaccgc cgcgattggc
      841 agccgcttgt cggcccagct ctactcactc gagatcatcc aagagagcat ccaggaccgc
      901 gccgagaacg cgacgcgctt cgtggtggtc gcgaagagcg atgccgagcg taccggccgg
      961 gacaagacga gcgtggcgtt ctcggcggcg cacgtgcgcg gggcgctgcg tcgtgcgctc
     1021 gaaatcttcg acgacgaagg actgaacctg actcgcatcg agtcacgccc cgcggccggc
     1081 cggcgctggg aatacgtgtt tttcaccgat ttcgaaggcc accgcaccga cgcgaacgtt
     1141 gccaaggcga tcgaccgcct gaggcacgct tgctcgacgg tccgggtgct cggcagctac
     1201 ccgcggaacg actcgcccta ggcggtgtcc ggcggcgggg ctgcgctctg ccggcgcggc
     1261 gcccgctcat cacgtagact cccgcggtca tgcaattgcg gaagttggtc ggctatttct
     1321 tgcgcggcgc tctggtcacg gcgccgctcg ggctcacgct gtacctcgtg ctcacggtgc
     1381 taacgacgct cgatcgcgtc ctaccagtgg gcatcccggg cctcggcttg ctcattacgc
     1441 tgctgctcat caccgccatc ggcgctttca cctcgaacgt ggtcggccgc agcgtcctcg
     1501 agtcgaccga gcggttcatg agtcgcgtgc cgctcgtgaa gctcgtctac aattcaatca
     1561 aagatctaat cggcgcgttc gtcggcgatc gaaagagctt cgacaagccg gtcgcggtgc
     1621 gacctcaacg cgactccgag ctgcgactgc tcggcttcgt cacgcgccaa gggctcggcg
     1681 tgctcggctt cccctcacac gtcgccgtat acctgccgca gtcttacaac ttcgccggcc
     1741 aactcgtcat cgtcccatcc gagctcgtcg aacccatcga cgtggcgagc ggcgagctga
     1801 tgaccttcat cgtttcgggc ggggtgtctg ggctcggtgt gggtcagtcg ttgttgccgc
     1861 caccgccgcg ctgaacggag ccgaggactt cggccatcac ctgcggtgag agcgacaaga
     1921 tctcgccgag acgagcgagc acccagcgct cactcgcggc ctccgacccg tccgcgtgaa
     1981 tcaagagggc tgcgttcgag aagaggagtt cgcgatcttc cgggctgagc tcctcgaggg
     2041 gaatgtcgtc ttcgagagtg cggcgagtgc gcgcgtactc caagagctcc tcctcttctt
     2101 ccggggtcgc atcgaacccg gacaggagcc cctcgatcac ctgggtctcc gaagcctcca
     2161 tccggccgtc ggcccacgcc accgcgatga gggactttac gatgttcttc tcgctcggcg
     2221 acacgattcc atcctcgtcg cgccctcgta cgaacgcaag tccattgcgc ctacaccccc
     2281 agcgcaccgg gtcaacgccg cgcgccttct tctttcgttc gaacgccggt cagaactctc
     2341 cacgcaggtc ggtcacggcg cccgagcaga cgagtaacgt ccaagctttc gacccgtgtc
     2401 cgctcgaaac cttcgagctc cgtcgagagc caatcccctt gcgctggcgc ttcaccgtca
     2461 atcaccacga agcgcccccc acatttcagc agccggccga ggctcgacag tgacaggttg
     2521 ggcccttcgc tacgacgaac atagagcacc aagtcggcgc tcgcggggag ctcggtgcgg
     2581 agcttgagct cgctcgcatc cgtcgcgacg aatgttacgt tttcataacc gcgctctcga
     2641 gcgcgtagct ccgcgagcgt cgggcgagcc gcttgttttg gctcaaacgc gagcacgcga
     2701 cgaaaaatcg gagcgagcac atcgagctgc tctcccgcgc ccggggcggc atcaatcgct
     2761 aggtcgcgcg ccagaacgac gtcgcggagc gccatcaaga acagcggcaa ctcgtcgctg
     2821 actcctggtg ctggccgcgg ctccccatcg cgctccaaca gctctcgcgt gcgcgcgtcg
     2881 cgccgacgaa tgatctctgg gatacgcgcg aacaccgcct cttcgtcgca gagagcac
//
LOCUS       DASTKS010000120         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_240827, whole genome shotgun sequence.
ACCESSION   DASTKS010000120 DASTKS010000000
VERSION     DASTKS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240827"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFQ35_02555"
     CDS             complement(<1..193)
                     /locus_tag="VFQ35_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929534.1"
                     /translation="MTSALHFVPVRGATKRVMGRPKARAAWRACLLWGGIYGASFVTN
                     PALRAAGPLFAIGFIVNGMR"
     gene            complement(389..769)
                     /locus_tag="VFQ35_02560"
     CDS             complement(389..769)
                     /locus_tag="VFQ35_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929535.1"
                     /translation="MLQFALGAIVVYGLFLVAGLWLRPKLALHRHQARLRAMSVSDLA
                     LEARRCADSAGYLKQFPDARLFAQSEAWTSASLAEALDELYASASSADRLANEAGPGS
                     FVYSWYDFGLASIREALEVTRPDS"
     gene            complement(1153..1371)
                     /locus_tag="VFQ35_02565"
     CDS             complement(1153..1371)
                     /locus_tag="VFQ35_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929536.1"
                     /translation="MSMRKFAALIPVCALFAACGGDATPEAKAPEGGAAAAEESKPAD
                     AKPEGDAAKPADGAPAEGGDAKPADEAK"
     gene            1600..2325
                     /locus_tag="VFQ35_02570"
     CDS             1600..2325
                     /locus_tag="VFQ35_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929537.1"
                     /translation="MARPQESPKLRLVGGGRGEARAFESEPPESSARASDGIGTDQGA
                     DHGAADEETSDAQLVALTASGQRDAFEGLYRRHSAFALGLAIRIQGGASDVEDVVHDA
                     FVQAHTRLADLKEPSAFRAWLGSIVVRLVRTRLRKRRLLNALGLASPDPVDLDSIASS
                     DATTEQRVLLAQVYALLQTLPASDRIAWTLRYIEKHRLEAVAELMGCSLATAKRRILR
                     AQRFLAGHFVPAFSKDSGDSDDS"
     gene            2322..3344
                     /locus_tag="VFQ35_02575"
     CDS             2322..3344
                     /locus_tag="VFQ35_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929538.1"
                     /translation="MIDDEVSKLQKSALRDYADDPRLARVWQRLHTGLAAKPARSRAA
                     LWLAPAFGVALFAAGVLVGRQSAPPGEMAPSVAAESPQANETIESTPAPLPAAAQEPR
                     SASVANPPRARVVRGRSAPALGVPVQNEPYAVEPAPYASAPAQLPEWQQKADAGDFVG
                     ARAALERSGGWDVALAGASPDQLMTLVDLGRASSERSQAIRALRRLIDAFPGAPEAPL
                     AAWTLGNQLEQSGDHAGAAEAYALYRRLSPTGDFAEDAAVRQADAALAQGDAARATEL
                     VDQYAKDFPNGRRLTELREELEKLNAESRKESDEKPAAATQAPPSAPAAPDAAPPSAE
                     GPEVTK"
     gene            3421..>3703
                     /locus_tag="VFQ35_02580"
     CDS             3421..>3703
                     /locus_tag="VFQ35_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929539.1"
                     /translation="MIEIDPAAEAILDARAVRRQVSLELSEIDVPSFGAGGGGAASAA
                     PSPPLFVRVLGRVDRQVEVELWARGELSGRRVVSGAESGQHLLVRRVGLA"
BASE COUNT          596 a         1139 c         1333 g          635 t
ORIGIN      
        1 ctcgcatccc gttcacgatg aacccgatcg caaacagggg tccggcggcg cgtagggccg
       61 ggttcgtgac gaacgaagct ccgtaaatac cgccccagag caaacacgcg cgccatgctg
      121 cgcgcgcctt gggcctaccc atgacgcgct tggtcgcgcc tcgtactgga acgaagtgca
      181 aggccgaggt catcgtctac ggcccgtcaa tttagcagag ccttcgatgt gtgctcgtga
      241 ccgtcggatg cttcgtcgcg gctctggtcg cttgcacgct aagggcccgc gaatgggtaa
      301 atcatactgc gcgagccgat cctcttgctg ctcgcgtatc tttgcgggca tgggtccagg
      361 atttgggact gtctcttggc cgagaacgtc atgaatccgg tctcgtgacc tcgagcgcct
      421 cacgaatgga tgcgagcccg aaatcgtacc aagaatagac gaagctcccc ggaccagctt
      481 cgttcgcgag gcgatctgcg gagctagcgc ttgcgtagag ctcgtcgagc gcctccgcga
      541 gcgatgccga agtccaagct tcgctttggg cgaagagacg cgcgtcggga aactgcttta
      601 gatagccggc tgagtcggcg cagcgacgcg cttcgagcgc gaggtcactg acggacatgg
      661 cgcgcaggcg cgcctgatgt cgatgcagcg cgagctttgg gcggagccag agcccggcga
      721 cgagaaagag gccgtagaca acgattgctc cgagagcaaa ctgcagcatc ggtcctttgg
      781 aagtcgcggt ccggagaggc ttaccggtac gggcttattg gtgcgcggct tgtcggtggg
      841 agcgcggctt gtcggtggga gcgcggctaa tcggtgggcg gcttgtcggt gggagcgcgg
      901 cgtatcggtg cgcggcttat cggtgcgggc gcgggcgcgg tggcggctgc gtgaggcgag
      961 cggcggagtc tgcgctgatg tcgagaagcg ccggtctgcg cgctctttac ggtgaggcgc
     1021 acctacccaa aagtgactgg agcgagcttg gaggcagagg gtgcgagcgc acaaaagcaa
     1081 acggccggag cgagtgaacg ctccggccgc tctgtggacg gtcgctacct cggggaggca
     1141 gcgacccaag tctcacttgg cctcgtccgc gggcttggcg tcgccacctt cggccggagc
     1201 gccgtcggcg ggcttcgccg cgtcgccttc gggcttcgca tcggccggct tcgactcttc
     1261 agcagccgcc gcgccgcctt ccggagcctt ggcttcggga gtggcatcgc caccgcaagc
     1321 agcaaagaga gcgcaaaccg ggatgagggc agcaaactta cgcatcgaca tttgggttcc
     1381 tcctgccgaa cccaatgggc tcggaccgac tgacagacgc cgaagtacca gaaagtggct
     1441 cacaggcgaa gagaactttg tcagatcgac ttcgctaagg tgaattattc caggtgcttg
     1501 taaaataaga acggctggta ggcaggtctt tgtgacgggc gcgtgagccg aagtggctag
     1561 tccgagcgtc caactgagtc gaagagtatg gtgtcggccg tggcccgccc gcaggagtct
     1621 ccgaagcttc gcctggtcgg tggaggacgg ggagaggcgc gtgctttcga gtcggagccg
     1681 ccggagtcgt cggcgcgtgc gtcggacggt atcggcactg atcagggcgc ggaccacggc
     1741 gctgcggacg aagagacgag cgacgcgcag ctggtcgcgc tgacggctag cgggcagcgc
     1801 gacgctttcg aagggctgta tcgtcggcat tctgcgttcg cgctgggtct tgcgattcgc
     1861 attcaaggcg gggcgagcga cgtggaagac gtcgtgcacg acgcgttcgt gcaggcacac
     1921 acacggcttg cggacttgaa ggagccttcg gcctttcgcg cttggctcgg cagcatcgtc
     1981 gtgcggctcg tgcgcacacg gctccgcaaa cggcggttgt tgaacgcgct cgggctggcc
     2041 tcgccggatc cggtggattt ggattcgatt gcttcgagtg acgcgaccac ggagcagcgg
     2101 gtgctcttgg cgcaggttta tgcgctgctg caaacgctgc cggcgagtga tcgcatcgcg
     2161 tggacgcttc gctatatcga gaagcaccgg ctcgaagcgg tcgcggagct gatgggctgc
     2221 tccctcgcga ccgcgaagcg ccgaattttg cgggcgcaac gttttctcgc cgggcacttc
     2281 gtgccggcgt tctccaaaga ttcgggcgat tcagacgact catgatcgac gacgaggttt
     2341 cgaagctgca aaagtcggcg ttgcgcgatt acgccgacga cccgcgtttg gctcgggtgt
     2401 ggcagcgctt gcatacgggc cttgctgcca agccggcgcg gagccgggcc gctttgtggt
     2461 tggcgccggc cttcggcgtg gcgctgttcg cggcaggcgt gttggttggt cgccagagcg
     2521 cgccgcccgg tgagatggcg ccttccgtcg cggccgagtc gccgcaggcg aacgaaacga
     2581 tcgaatcaac accggcgccg ttgccggcgg ccgcgcaaga gcctcgctct gcaagcgtcg
     2641 cgaatccgcc ccgagcgcgc gtggtccgtg gtcgcagcgc acccgcgctc ggcgtgcccg
     2701 ttcagaacga gccgtacgca gtcgagccag cgccctacgc ttccgcaccc gcgcagctgc
     2761 cggagtggca gcagaaagct gacgcgggcg acttcgtcgg ggcgcgggcg gcgctcgagc
     2821 ggtcgggcgg ctgggacgtt gcgctcgcgg gcgcatcgcc agaccaactc atgacgttgg
     2881 tggacttggg gcgcgcgagc tccgaacgct cgcaagcgat tcgcgcgctg cgccggttga
     2941 tcgatgcgtt ccccggcgcc cccgaagcgc cactcgcggc gtggactctc ggcaaccagc
     3001 tggagcagag cggcgaccac gcgggcgcgg ccgaagcgta cgcgctgtat cggcgcttgt
     3061 ctccgaccgg cgacttcgcc gaagatgcgg cggttcgcca agccgacgct gccctcgctc
     3121 agggggacgc ggcgcgagcg accgagctcg tcgatcagta tgcgaaagac ttcccgaacg
     3181 gtcggcggct cacggagttg cgtgaggagc tcgagaagct caacgccgag tcgcgcaaag
     3241 agagcgacga gaagcctgct gccgcgaccc aagctccgcc gagcgcgccc gcggcgcccg
     3301 acgcggctcc gccttccgcc gaaggtccag aagtcaccaa gtgattggtg ggcgctcgtc
     3361 gcgctcgtcg ccaccgtact tttcgctccc cagcgcgtca ccgcgcaacc gaaccagtcg
     3421 gtgatcgaaa tcgatccggc ggcggaggcg atcctcgatg cgcgcgcggt gcgccgccag
     3481 gtgtcgctgg aactctcgga gatcgacgtg ccttcgtttg gcgccggagg cgggggcgcg
     3541 gcgagcgcgg ctccgtcacc gccgttgttc gtgcgggtgt tgggacgcgt ggaccgacag
     3601 gtcgaggtcg agctctgggc gcgcggcgag ctgagcggtc ggcgtgtcgt ctccggtgcg
     3661 gagagcggtc agcacctgtt ggtgcgccga gtcgggctcg ccg
//
LOCUS       DASTKS010000121         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_911171, whole genome shotgun sequence.
ACCESSION   DASTKS010000121 DASTKS010000000
VERSION     DASTKS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911171"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..2212
                     /locus_tag="VFQ35_02585"
     CDS             104..2212
                     /locus_tag="VFQ35_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929540.1"
                     /translation="MKRLQALLTKLARRAQRSVSLEEALLTSRGFAYVWYRARFLLLR
                     VPFGMLLHAAEVVAFSAVYDLGLIGPMLTIRSLPAVAAALWWGALEELRQRVRDAAAK
                     GEWGRAERAVRSFLGLAVDVGLAALAVAFVYVEFVPSVFGGFDIFDAYAIACSLRFAA
                     ETVARTYHAGVFALRRVYRPWWSLLLPDFCEVFVALVLWPSLGPWGFSFSTLVGGLLG
                     ALLQLHYSRLAYNEQGQFRPSLQRLHKARGDIVRRDFVELARHALANLASHVDALLVV
                     LLSRSLQSGLPFVVLLHAGRPLFSAVSSYARLFYFDFQRLELGGSRFFVQRFARLVSR
                     FAVALAGVAIALTFVLYGVAFTELEGKLLIDFALLAIYVVVRSTLALEQIRSFTAGRY
                     RPLAQGAVALIAAAVVLRLTISRERLLIATLVASLALLATWLWYKNRGTRPGAPQTNV
                     SFLRWLQALRERIEPVEIGYAVVDLHSGASVNAVQHALSEELSKCAAAHPDLGSQSPV
                     LTRASKRLLVWYAPPNANHAELRHRLLEATAGCVEALELSGPHSADRALSAALALPPL
                     ARVVSGSDVPTGSEPVDSDSTQDLASIADLRAHFENTFVRGATLELDRAPTRSFLPNR
                     TARSFVARSVFAACAGRPAPSRRPEFPRVAVYCPSAEPQLVFLAPPACNPADFRRFRD
                     SVDALSLRETLSPSVTSRRA"
     gene            2589..>3068
                     /locus_tag="VFQ35_02590"
     CDS             2589..>3068
                     /locus_tag="VFQ35_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929541.1"
                     /translation="MKTPVGRRCAATLVVLLLAGTSSIGCKRTEATTSQTTQAEAAAT
                     AGAVPAAAPAGAALTGFDPCLVGSFEAEKVTLKLDQLSAEGGSKVALEIAVNGASTID
                     FTPMSEIHAKASRGLAFDFKYAGKATSTLKTPTRGTIVSESTNLDALRVNATIKLPGA
                     "
BASE COUNT          470 a         1071 c          949 g          578 t
ORIGIN      
        1 gccctgcctg ctacgctccc gcgccgcgcc cagcgatgcg cccggtgaaa cgcctccaag
       61 ccttgcctgc tacgctcccg cgccgcgccc agcgaggcgc ccagtgaaac gcctccaagc
      121 cctgctcaca aagctcgccc gtcgcgcgca acgcagcgtg tcgctcgagg aggcgctgct
      181 cacttcacgt gggttcgcgt acgtctggta tcgcgcgcgt tttctcttgc tgcgcgttcc
      241 gttcggcatg ctgttgcacg ccgccgaggt ggtcgcgttc tcggccgtgt acgatctcgg
      301 tctaatcggc ccgatgctca cgattcgcag cctacccgcg gtcgcggcgg cgctctggtg
      361 gggcgcgctc gaagagctgc gccaacgcgt gcgcgacgcg gcggcaaagg gcgagtgggg
      421 gcgcgccgaa cgcgccgtcc gtagctttct gggtctcgcc gtcgacgtcg ggctcgccgc
      481 gctcgcggta gcgttcgtgt acgtcgagtt cgtgccttcg gtattcggag gcttcgacat
      541 cttcgacgcc tacgccatcg cgtgcagctt gcgcttcgcc gccgaaaccg tcgctcgcac
      601 ttatcacgcc ggggtcttcg cgttgcgccg cgtgtatcgc ccctggtgga gcctgttgct
      661 ccccgatttt tgcgaagtct tcgtggcgct cgtgctctgg ccatcgctcg ggccctgggg
      721 tttctccttc tcgacgctcg tgggcggcct gctcggggcg ctgcttcaac tgcactactc
      781 gcgcctcgcc tataacgagc agggccaatt ccgcccgagc ctgcagcgcc tgcacaaggc
      841 tcggggcgac atagtgcgtc gcgacttcgt cgagctcgct cggcacgcgc tcgcaaacct
      901 tgcatcgcac gtggacgcgc tgctcgtcgt gctactcagc cgctcgctcc aaagtgggct
      961 tccgttcgtc gtcttgctgc acgcggggag gccgctgttc tcggcggtca gcagctacgc
     1021 gcggctcttc tatttcgatt tccagcgctt ggagctcggc gggagccgct ttttcgtgca
     1081 gcgcttcgct cgcctcgtct cccgctttgc ggtggcgctc gccggcgtcg ccatcgcgct
     1141 caccttcgtg ctctacggcg tcgctttcac ggagctcgaa ggcaagctcc tcatcgactt
     1201 cgcgctgctc gccatctacg tcgtcgtgcg ctcgaccttg gcgttggagc agattcgttc
     1261 attcaccgcc ggccgttacc gaccgctcgc gcaaggcgcc gtcgcgctca tcgccgccgc
     1321 ggttgtcttg cggctcacca tctcgcgaga acggctgctc attgcgacgc tcgtcgcgtc
     1381 actcgcgctc ctggcgacgt ggctctggta caagaatcgc gggacgcgcc cgggtgcacc
     1441 ccaaaccaac gtctcgttcc ttcgttggtt gcaagccctg cgcgaacgaa tcgagcccgt
     1501 cgaaatcggc tacgccgtcg tcgatttgca cagcggcgcg agcgtcaacg ccgttcagca
     1561 cgcgctctcc gaagagcttt cgaaatgcgc cgcggcgcac ccggacctcg gatctcagag
     1621 cccagtgctc acccgcgcta gcaaacgtct actcgtttgg tacgcgcctc ctaacgcgaa
     1681 ccacgcagag ctgcgccacc ggttgctcga agcgacggcc ggctgcgtcg aagctctgga
     1741 gctcagcggt ccccactccg cagaccgagc gttgtccgcc gcccttgcgc taccgccgct
     1801 cgctcgcgtg gtctcgggtt ccgacgttcc gacagggagc gaaccggtgg atagcgactc
     1861 aacgcaggat ttggcctcga ttgcggatct ccgcgcacat ttcgaaaata ctttcgttcg
     1921 cggtgccacc ctcgagctcg atcgcgcgcc gactcgaagc ttcctaccga accgcaccgc
     1981 gcgctcgttc gtcgcgcgca gcgtcttcgc cgcatgcgcc gggcgccctg cgccgtcgcg
     2041 ccgacccgag tttccacgcg tcgccgtgta ctgcccctct gcagaaccgc agctcgtctt
     2101 cttggcgccg cccgcctgca atcctgctga ttttcggcgc tttcgcgact cggtcgacgc
     2161 gctctcgctg cgcgaaaccc tttctccgag cgtcacttct cgtcgcgctt agcacggcgt
     2221 tgaaaaacgc cctgcgtcaa gacagaccaa tgccaggcac acacgacgcg cagaagcaag
     2281 catcacgaaa gcctcacatt catgggcttt ggttgacgcg cagccgctcg gtggtgccgg
     2341 tcagcattct agcagggcgt ttttcaacgc cctgttaggg cccgcgaatg aacaagtcag
     2401 tcgcgagcgg gccgagaccg cagcgaggcg ggcgccgtga ggagagagca ccgcaggcct
     2461 gctgaagcag gtcgaggagc gcaacgacga gcccgcacgc cgcagcaaga ggatcggccc
     2521 gcgcagtatg acttgtccat tcgcgggccc ttacccattc gcgcgccata aagtgataaa
     2581 aggaaccgat gaagacacct gtggggagac gatgtgcagc gacgctcgtg gtgctattgc
     2641 tggcggggac atcgagcatc ggttgcaaga gaacggaggc gacgacgtcg cagacgacgc
     2701 aggccgaagc tgcggccacg gccggagccg tacccgcggc cgcgccagct ggcgccgcgc
     2761 tcactgggtt cgatccgtgc ctcgtcggta gcttcgaggc cgaaaaggtg acgctgaaac
     2821 tcgatcagtt gagcgcagag ggcggctcta aagtcgcgct cgagatcgca gtgaacggcg
     2881 cgagcaccat cgatttcact ccgatgagcg agatccacgc gaaggcgagc cgcggcttgg
     2941 ctttcgactt caagtacgcg ggcaaggcga cgtcgaccct caagacgcca acgcgcggca
     3001 caatcgtgag cgagtcgacc aacctcgatg ccttgcgcgt caacgcaacg atcaagctgc
     3061 cgggcgct
//
LOCUS       DASTKS010000122         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1134484, whole genome shotgun sequence.
ACCESSION   DASTKS010000122 DASTKS010000000
VERSION     DASTKS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134484"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1086)
                     /gene="cheB"
                     /locus_tag="VFQ35_02595"
     CDS             complement(16..1086)
                     /gene="cheB"
                     /locus_tag="VFQ35_02595"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HET9929542.1"
                     /translation="MSKTRVLVVEDSLTVRQRLLEVLRSDPDLEVVGDAGDGRTAIEL
                     CEKLRPDIITLDMMLPVMTGLAATEHIMAFCPTPILIVSSSTNRGELFKTYQALAAGA
                     VEVLEKPRGDEGEGVWERDFLRLVKLVARIKVITHPRARLSLSRGDSVRAPQTASPGG
                     RARCVAIGASTGGPAAVARVLQSLPADFPVPILLVIHIGAIFASGLAEWLDTQSPLRV
                     VHAKHGEPLPPPGEGRVLMAPPDRHLVVRRGAIELVSGAERHSCRPSIDVLFESLAVE
                     NGAQVVACLLTGMGRDGAAGLLALRQAGGTTIAQDEATSVIYGMPREAVVLGAAQEVL
                     PLDRIGPALVAATTRRGSHRPI"
     gene            complement(1093..3177)
                     /locus_tag="VFQ35_02600"
     CDS             complement(1093..3177)
                     /locus_tag="VFQ35_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929543.1"
                     /translation="MADDPYKYFRIEARELCEQIGSGVLELERAFDPELIARLLRLSH
                     TLKGAARVVKQGDIAREAHTLEEALLGKRAAQASASVEEVRSLLALVDRVSRLVNALS
                     DAGESERRVQSVAPQAAEAQRDPASRPIESAQMLRVDPQQVEELVRSTSNASQCMAAL
                     EQELGGFQRIASLASELAEACSPRFERAASGAALGRLRSLVSELGASVERLQRGTLGA
                     AAEAAAELDAVREAAHQLRLLSASTVFPALERAVYDAAAELGKRAVIETFGGDIRLEG
                     QVLTALRDCLLHVVRNAVAHGIETPSERLLAGKPERGVVRIEVRRTGSRVVFRCRDDG
                     RGIDLRAVKRAVVERGLVPEGEAQTLSESQVMRLLLQSRISTSDSVSEIAGRGIGLDA
                     VSETVRGLRGAIRLDSVSGQGATVELDVPLSVAAISALLVEVNGATVAIPLDAVEATA
                     RIKAQELVRTDRSESILFDEELLPYAPAQRALKFHSTAVKQANVLALVLLRSARGRAA
                     ISVDRVLTTGKVVLRALPRSVCAAPVVAGGALDAAGNALIVLDADALVDFVTHAPLAE
                     PATELRAPAPVLVIDDSLTTRMLEQSILESAGYVVELAQSAEEGLEKARSKTYGVYIV
                     DVEMPGMNGFEFVAATRADPALSQTPAILVSSRDGSEDKARAARVGARAYIVKGEFEQ
                     EHLLSTIRSLMS"
     gene            complement(3189..>3990)
                     /locus_tag="VFQ35_02605"
     CDS             complement(3189..>3990)
                     /locus_tag="VFQ35_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9929544.1"
                     /translation="SAELQAAANQQATGAREQATAMSEITTTISELLATSKQIAESSQ
                     RVAAIATDTRTAAQTGDQLVQRTTDSIGGMRRQIDAVVAHMLDLGRKSQQIGGILEII
                     NELAEQTNILAINATIEAAGAGDAGKRFGVVADEVRRLADRVGSSTREIRGLIDEVRA
                     AVNTTVMATEGGTKAVEAGARSFSDAAQAFSQIVALVSTTSEAAREIELSTKQQSTAV
                     EQVNQAAASVAQATKENEASSSQTLQTASELAGLSRELLKVVQSGTTA"
BASE COUNT          663 a         1364 c         1307 g          656 t
ORIGIN      
        1 cggctcgagg ccacctcaaa tcggacggtg cgatccgcgt cgcgtcgtcg ccgctacgag
       61 cgccggaccg atgcgatcga gcggaagcac ctcttgcgcc gcgccgagca ccaccgcctc
      121 gcgcggcatt ccgtagatga ccgaggtcgc ttcgtcctgg gcgatcgtcg tgccgcccgc
      181 ctgacgcaac gccaagagcc ccgcggcgcc atcgcgcccc atgccggtca gcaggcacgc
      241 gaccacctgc gcgccatttt cgaccgccaa tgactcgaac aagacgtcga tggatggccg
      301 gcaagaatgc cgctccgcgc ccgagacgag ttcgatggcg ccccgacgaa cgaccaaatg
      361 cctatcggga ggagccatca agacgcgtcc ctctccgggc ggcggcagcg gctcgccgtg
      421 cttcgcgtga acgacgcgta gcggcgattg ggtatcgagc cactcggcga gcccagacgc
      481 gaagatggcg ccgatgtgaa tgacgagcag gatgggtacc ggaaagtccg cgggcagaga
      541 ctggagcacg cgagcgaccg cggcaggccc accggtagaa gcaccgatcg ccacgcatcg
      601 cgcgcggccg ccgggggaag cggtctgcgg cgcgcgcacg gagtctcccc gcgagagtga
      661 cagccgggcg cgcgggtgtg tgatcacctt gatgcgcgca acgagcttga cgaggcgcaa
      721 gaagtcacgc tcccagaccc cctcgccttc gtcaccacgc ggcttctcca gcacttccac
      781 tgcgccggcg gcgagggcct gataagtctt gaagagctca ccgcggttcg tggacgacga
      841 gacgatgagg atcggcgtcg ggcagaacgc catgatgtgt tcggtcgcag ccaggcccgt
      901 catgaccggc agcatcatgt ccagggtgat gatgtccggc cgcagcttct cgcagagctc
      961 gatcgcggtg cgaccgtccc ccgcgtcccc gacgacttcg agatcggggt cactccgcaa
     1021 gacttcgagc aggcgctgcc gcaccgtcag cgaatcttcg acaacgagaa cgcgcgtctt
     1081 cgacatcgcc gttcaactca tgagtgaccg aatggtagac aacaagtgct cctgctcgaa
     1141 ctcacctttg acgatgtagg cgcgcgcacc gacgcgggcc gcgcgcgcct tgtcctccga
     1201 gccatcccgc gagctcacca agatcgcagg cgtctgcgac agcgccgggt cggcgcgggt
     1261 cgccgcgacg aactcgaacc cgttcatacc cggcatctcg acgtcgacga tgtagactcc
     1321 gtaggttttc gagcgggcct tttccaagcc ttcctccgca gactgcgcca gctcgacgac
     1381 gtagccggca gactcgagaa tactctgttc gagcattcgt gtcgtgaggg agtcgtcgat
     1441 cacgaggacc ggagccggcg cacggagctc ggtggccggc tccgcgagtg gggcgtgcgt
     1501 gacgaaatcg actagggcgt cggcgtcgag cacgatcagc gcgttcccgg ccgcgtcgag
     1561 cgcaccgccg gccactacgg gtgcggcgca caccgaacgg ggcagagcgc gcaggactac
     1621 cttgcccgta gtgagcaccc gatcgaccga aatggcggcg cgcccacgcg cggagcgcag
     1681 cagcaccaac gctagaacgt tcgcttgctt cacggccgtg ctgtgaaatt tcagcgcacg
     1741 ctgggccgga gcgtagggga gaagctcctc atcgaacaga atcgattccg acctatcggt
     1801 gcgcacgagc tcttgcgcct tgatgcgagc cgtggcctcg acggcgtcga gcggaatcgc
     1861 gacggtcgcg ccgttgacct cgaccaacag agccgagatc gccgccaccg atagcggtac
     1921 gtccagctcg accgtggctc cctgccccga aacgctatcg aggcggatcg ccccgcgaag
     1981 cccgcgcacg gtctcgctga ctgcgtcgag gccgatccct cgcccggcga tctcgctcac
     2041 gctatcgctc gtgctgatgc gcgactgcag caagagccgc atgacttggc tctccgagag
     2101 cgtctgcgct tcaccttcgg ggacgagccc tctttcgacg acggcgcgct tcaccgccct
     2161 gagatcgatg cctcggccgt cgtcgcgaca acgaaagact actcggctgc cagtgcggcg
     2221 aacctcgatt cgaacgacgc cacgctccgg cttgcccgcg agcaggcgct cggaaggtgt
     2281 ctcgatgccg tgcgcgacgg cgttgcgcac gacgtgcaga agacaatcgc gaagggccgt
     2341 gagcacctgg ccttcgaggc ggatgtctcc gccaaaggtc tcgatgaccg cgcgctttcc
     2401 gagctccgcg gcagcatcgt aaacggcccg ctcgagcgcg ggaaatacgg tcgacgccga
     2461 gagcagtcgc agctggtgcg cggcttctct caccgcgtcg agctcggccg cggcctcggc
     2521 cgcagcgccg agggtgccgc gttgcagtcg ctcgacggaa gccccgagct cactcacgag
     2581 agaccgtagg cgaccgagcg cagcgccgct cgcggctcgc tcgaagcgcg gcgagcacgc
     2641 ttccgctagc tccgaagcta gcgaagcgat gcgctggaag ccgccgagct cttgctcgag
     2701 agccgccatg cactgcgagg cgttactggt ggagcgcacg agctcctcca cctgctgcgg
     2761 gtcgacgcgc aacatctgag cgctctcgat gggtcggctc gcggggtcgc gctgcgcttc
     2821 ggcagcctgc ggcgccacgc tctgaacccg gcgctcgctt tcgccggcgt ccgaaagcgc
     2881 gtttacgagc cggctcactc gatccacgag cgcgagcagc gagcgaacct cctcgacgct
     2941 ggccgaggcc tgggccgcgc gcttgccgag cagcgcctct tcgagagtgt gcgcttcgcg
     3001 cgcgatgtcg ccctgtttga cgacacgagc tgcacccttc agcgtgtgag agagccgcaa
     3061 gagccgcgcg atcaactccg gatcgaaagc gcgctcgagc tcgagcacac cgctcccgat
     3121 ctgctcgcaa agctcccgag cttcgatgcg aaagtacttg taagggtcgt cggccacgcg
     3181 tggcgagcct acgcggtcgt ccccgattga acgactttca ggagctcgcg agagagcccc
     3241 gcgagctctg aagcggtttg cagcgtctga ctggagctcg cctcgttctc cttggtggcc
     3301 tgtgcgacgc tcgcggcggc ctggttgacc tgttctaccg cggtggattg ttgtttcgtg
     3361 ctgagctcga tctcacgagc ggcctcgctg gtcgtcgaga cgagcgcgac gatttgcgag
     3421 aaagcttggg cggcgtcgga aaacgagcgc gcccccgctt cgacggcttt cgttccaccc
     3481 tcggtcgcca tcacggtcgt gttcaccgcc gcacgcacct cgtcgatgag gcctcgaatt
     3541 tctcgcgtcg agctgccgac gcgatcggcg aggcgccgca cctcgtcagc gacgacaccg
     3601 aagcgtttcc ccgcgtcgcc cgcacccgcc gcctcgatgg tggcgttgat cgccaaaatg
     3661 ttggtttgct cggcgagctc gttgatgatc tcgaggatgc cgccgatttg ctgcgacttt
     3721 cttccgaggt ccagcatgtg cgcgacgaca gcgtcgattt gtcgacgcat tccgccgatc
     3781 gagtcggtcg tacgctgcac cagctgatcg ccggtttgag ccgcggtgcg cgtgtcggtg
     3841 gcaatcgcgg cgacgcgctg cgagctctcg gctatctgct tggaggtcgc caagagctcg
     3901 ctgatggtcg tcgtgatctc gctcatggcg gtcgcttgct cgcgagcgcc ggtcgcctgt
     3961 tgattcgcgg cggcctgaag ctccgccgag
//
LOCUS       DASTKS010000123        10200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1803735, whole genome shotgun sequence.
ACCESSION   DASTKS010000123 DASTKS010000000
VERSION     DASTKS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803735"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFQ35_02610"
     CDS             complement(<1..570)
                     /locus_tag="VFQ35_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9929545.1"
                     /translation="MTRTPEAPSALPPEWPELLSELQALGMRWRSSDGPGLFRRGGAG
                     PSDHKAVTLASHTVMVPIFTHASARSPYSAEVEPGSERATLLRNERPVAEISFPKAPR
                     FYALSTPDGIPYWKIATLHASDVLASTVLQTCTRYGHRETSCQFCAIGKSLAAHATIP
                     EKTPEQLAAVAAAAVALDGVKHAVLTTGTP"
     gene            complement(521..1570)
                     /locus_tag="VFQ35_02615"
     CDS             complement(521..1570)
                     /locus_tag="VFQ35_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitrilase"
                     /protein_id="HET9929546.1"
                     /translation="MEEKPKVVRAAAVQLSPVLYSCAGTTQKVCEAIAEAARNGAELI
                     VFPETVIPYYPYFSFIKAPALIGADHLLLFEQSVTIPGPVTEALSAAAQRAGAVVVIG
                     VNERDHGTLYNTQLIFDADGRLVQARRKITPTYHERMIWGQGDGSGLVAVDTRVGRVG
                     ALACWEHYNPLARYALMVDHEEIHVAMFPGSLVGDIFREQIEVTIRHHALESGCFVVN
                     ATAFLDEGQVAEIAGNTNLEKALRGGCFTAIVSPEGTLLSKPITEGEGIAYADLDMRL
                     VLKRKRMMDSVGHYSRPELLSLSIDRTEYAHAREASSNAAALVVTAPRNFDASNSADR
                     DTNARSAERAAARVA"
     gene            complement(1611..2093)
                     /locus_tag="VFQ35_02620"
     CDS             complement(1611..2093)
                     /locus_tag="VFQ35_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HET9929547.1"
                     /translation="MPKIEAPLNKSGDFLVDYEEKVFEDVKAEPGEKALVTFHTVAFE
                     GSIGLVNLLQAIRLQRKGFETSVLLYGPGVTLGVQRGFPKLGDEAFPGHLNFNGQIKK
                     FMAEGGKVYACRFALQALYGHGEKALIEGIRPINPLDVLDIILLHRKQNAFILDTWTV
                     "
     gene            complement(2346..3911)
                     /locus_tag="VFQ35_02625"
     CDS             complement(2346..3911)
                     /locus_tag="VFQ35_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HET9929548.1"
                     /translation="MLGPGMQLEALQSLALSVAQARSPDSVLTEMVQGLGMWEGVALA
                     RVWLLREDDRDGAYLELKASIGASIVDLDRRWNRIDGSHRRIPLSFGKVGHIAASNRP
                     LLLQRGPGNWIVEPEWAEAEGIQSFAGQPLCFRGQVLGVVAFFSRRRLGKSDLDWLRV
                     FADHAAVAIANARAFAEIERLKESAERERDYLREAVRSALQHGDIIAESPGMRRVLEQ
                     LRAVAAADASVLILGESGVGKELIAAAVHDLSQRRDGPLVKVNCASVPRELFESEFFG
                     HVRGAFSGAARDRQGRFLLADGGSLFLDEVGEIPIELQGKLLRVLQERTFEAVGDDRT
                     RRVDVRVIAATNRPLETEVEAGRFRRDLFYRLNVLPIEVPPLRERREDILPMARRFLE
                     TTAHRLHLAVPELSKADEAALLAYDYPGNVRELQNVLERALVLMQRDGVLRLGLGRPM
                     SVPPPESARRPVQDDVLPEHVMREFERKNLQRALERCGYQISGAAGAAKLLGISPSTL
                     AYRMKQLGLERPR"
     gene            complement(4080..4484)
                     /locus_tag="VFQ35_02630"
     CDS             complement(4080..4484)
                     /locus_tag="VFQ35_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929549.1"
                     /translation="MHTSKNRQDEQLERAVDSAPGAVAPTGGAVPEDAVPKRAAPGHD
                     LDELVDTLGLLLRAARQLDPERRETKLRALEKEAESVITAIEEIDGTGADEVRHQTAA
                     RVAESTRRELMPVIEHTLERLRSNLRAETSPD"
     gene            complement(4519..4773)
                     /locus_tag="VFQ35_02635"
     CDS             complement(4519..4773)
                     /locus_tag="VFQ35_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929550.1"
                     /translation="MTTNSSGNSHSNVIESIAQLRDEIRLKVHLAGLDAKEKWQALEE
                     QVQNLEHRVSADGGSLMDATATLARDLKQSLEHFRERLAH"
     gene            complement(4981..6570)
                     /locus_tag="VFQ35_02640"
     CDS             complement(4981..6570)
                     /locus_tag="VFQ35_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9929551.1"
                     /translation="MDADVLLSDLRRWEAYLPVLGGDARERPVTLIETHISRVFLIGE
                     HVFKLKKPVDFGFLDFTTLELRRRACEAEVLLNRRLAEGVYLGLVSVTRVAGGHHELG
                     GGGELVDYAVHMKRLPDERRADRLLVAGAFEHRELDDVAQRLADFHAELSAEPERRHW
                     GSVEAVRGNVEENFAQTREAIGRYLEPEQAAELERFQKDFLRERAELFTRRMNEGRIR
                     DGHGDLRLEHVYMLGDGVAIIDCIEFNERFRFADVCADVAFLSMDLAEHGRVDLAERL
                     LARYALLSNDFGLYALVDFYQSYRAHVRAKVAGFQALSPDVDVMTRERSDAEARRHYL
                     LALSAARRPLARTALIVVCGVIASGKSHVAEALAAELALPVIVADRVRKAHAGVSAET
                     PLSDPSFASNYSSEATRRVYEELREGATTVLASGRSVILDASFRSRSERLAVRELAQR
                     VGAAFVLVECRASREVCLARLQKRAEGRSVSDGRIEIFDDFARSFDPIEELGPSEHVV
                     LDTELALAENVRRVRACLPFG"
     gene            6757..7413
                     /locus_tag="VFQ35_02645"
     CDS             6757..7413
                     /locus_tag="VFQ35_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9929552.1"
                     /translation="MDKPSKPFVAVVGYDFSELADLALDQCLDVVSRHPEAEVHVVYV
                     VPAVALTANPLAAALPSAVSGDVLDDTAARLKKAVDDRLAHYVKTHQPLTCRVVSHLR
                     VDETASCIAQLAADLESDILVVGTHSRRGVARLLLGSVAQSVIALAPCPVLVVRPKRI
                     EAHSPAIEPPCPRCVETRQATQGAEFWCEQHRERHGRRHTYHQDDRTSSDSTLPLVVH
                     "
     gene            7565..9814
                     /gene="ccoN"
                     /locus_tag="VFQ35_02650"
     CDS             7565..9814
                     /gene="ccoN"
                     /locus_tag="VFQ35_02650"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HET9929553.1"
                     /translation="MTTHVEKFSYDDATVRKFAFATAIWGVVATLAGLWLAVMLVAPS
                     LSFGLPQLTFGRLRPLHTNAAIFAFAGNAVFAAVYYSSQRLLKARMFSDALSKFHFWG
                     WQAIIVAAALTLPFGITQSKEYAELEWPIDLAIAVVWVAFGINFFGTVAKRRERHIYV
                     AIWFYMATVVTVAILHIFNNISIPAGALKSYSVYAGVQDAFMQWWYGHNAVAFFLTTP
                     FLGLMYYFLPKAANRPVFSYRLSILHFWSLVFIYIWAGPHHLHYTALPEWASTLGMLF
                     SVMLWMPSWGGMINGLLTLRGAWNRVSDDPVLKFFVVGITFYGMSTFEGPMLSIKSVN
                     ALSHYTDWTIAHVHSGALGWNGFMTFGMLYWLAPRIFQTKLWSKKLAEVHFWLGTLGI
                     LLYIVAIYAAGVTQGLMWRAFDQTGRLAYPDFVETVVRILPMYWVRALGGLLFVTGVV
                     LGLVNIVMTWKTRPATYTEPTDEAPALTPYTGETTRFVIPRHSTISNFGHAVRYLAEA
                     RWHRVWERKPLKFALWVAASVIIASLFEIIPTFLIRSNVPTIASVKPYSPLELLGRDI
                     YVSEGCYNCHSQMVRPIRAETERYGEYSKPGEFVYDHPFQWGSRRIGPDLHRVGGKYP
                     HLWHVRHMQDPRSTTPQSIMPAYPWLASNDLDFSVVQKRVDVMAMLGVPYGDAVNHAE
                     ELAQAQAHRIASEIEAQGGPRGLEQKQIIALVAYLQRLGTDIKAPVNGDKPARIARSE
                     GAATGEVIK"
     gene            9811..10011
                     /locus_tag="VFQ35_02655"
     CDS             9811..10011
                     /locus_tag="VFQ35_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929554.1"
                     /translation="MKLSDIVSALHLSIFAEVPLIICFGIFLGVVLPVMRSREEFEQM
                     RTLPLRNDEPETDPTRRQGGSP"
     gene            10008..>10200
                     /locus_tag="VFQ35_02660"
     CDS             10008..>10200
                     /locus_tag="VFQ35_02660"
                     /inference="COORDINATES: protein motif:HMM:NF026065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HET9929555.1"
                     /translation="MNEPVSTSSRPPSKAPPAEEHRADTASVLDHEYDGIREYDNPLP
                     RWWVNIFWGSFVFAIGYFFH"
BASE COUNT         1785 a         3414 c         3141 g         1860 t
ORIGIN      
        1 cggagtgccc gtcgtcagga ccgcgtgctt cactccgtcg agcgcgaccg cggccgcggc
       61 gactgcggcg agctgctccg gtgtcttctc cgggatcgtt gcgtgcgcgg cgagggactt
      121 gccaatcgcg cagaattggc aggacgtctc gcgatgcccg tagcgagtgc aggtttgcag
      181 cacggtgctc gcgagcacgt cggaggcgtg cagcgttgca atcttccagt atgggatgcc
      241 gtccggagtg ctcagcgcgt agaagcgcgg agctttgggg aagctgattt cggcgacagg
      301 ccgttcgttt ctgagcagcg tggctcgctc gcttcctggc tcgacctcgg cgctgtaagg
      361 cgagcgcgcc gacgcatgcg tgaagatcgg caccatgacg gtgtgcgagg cgagggtgac
      421 ggctttgtga tccgacgggc cagcgccgcc gcgccggaac aagccgggcc catccgatga
      481 gcgccagcgc attccgagcg cttgcaactc cgagaggagc tcaggccact cgggcggcag
      541 cgcgctcggc gcttcgggcg ttcgtgtcac gatcggcaga gttcgatgca tcgaagttcc
      601 tcggtgcggt cacgacgagc gcggcagcgt tgctcgaagc ctcgcgcgcg tgagcgtatt
      661 cggtacggtc gatcgagagg gaaagtagct ccggacggct gtagtgaccg acgctgtcca
      721 tcatgcgctt tcgcttgagg acgaggcgca tgtcgaggtc cgcatacgct atcccttccc
      781 cttcggtgat tggcttcgag agcagagtgc cttcgggcga gacgatcgcc gtgaagcacc
      841 caccccgcag cgccttttcg aggttcgtgt tccccgcgat ctcggcgacc tgtccctcgt
      901 cgaggaacgc ggtcgcgttg accacgaagc agccggactc gagcgcgtgg tgacgaatcg
      961 tcacctcgat ttgctcgcgg aagatgtccc cgacgaggga tccggggaac atcgcgacat
     1021 gaatctcctc gtggtcgacc atcagcgcat aacgcgcgag tggattgtag tgctcccaac
     1081 aagccagcgc gccgacgcgg ccaacccgcg tgtccaccgc gacgagaccg gagccgtcgc
     1141 cctgccccca gatcatccgc tcgtgatacg tgggggtgat cttgcggcgc gcttgcacga
     1201 gccgaccgtc ggcgtcgaag atgagctgcg tattgtacaa ggtgccgtga tcgcgctcgt
     1261 tcacgccaat cacgaccacg gctcccgcgc gctgcgcggc tgcggacagg gcctcggtga
     1321 cgggccctgg aatcgtgacc gactgctcga atagaaggag gtggtcggcc ccgatgagag
     1381 caggcgcctt gatgaacgag aagtacggat aataggggat caccgtctcg ggaaagacga
     1441 tgagctctgc cccgttccgt gctgcctccg cgatcgcttc gcagactttt tgggtcgtgc
     1501 ccgcacagct gtacaaaact gggctgagct gcaccgcggc cgctcgcacg actttcggtt
     1561 tctcttccat tgacgtcatc ctggctcccg ctccgtccgc aaccgccggc tcaaaccgtc
     1621 caggtgtcga ggatgaaggc gttctgcttc ctgtggagca agatgatgtc gagcacatcg
     1681 agcggattga tgggtcgaat cccctcgatc aacgcttttt cgccgtgtcc gtagagtgcc
     1741 tgcagcgcaa accggcaggc gtagaccttg ccaccctcgg ccatgaactt cttgatttga
     1801 ccgttgaagt tcaagtgtcc ggggaacgcc tcgtccccga gtttcgggaa gccgcgctgc
     1861 acgccgagag tgacgcccgg cccgtagagc agaacggacg tttcgaatcc ttttcgctgc
     1921 aagcgaatcg cctgcagaag gttcacgagg ccgatcgagc cctcgaacgc gaccgtgtga
     1981 aacgtaacca gagccttttc ccccggctcc gctttgacgt cttcgaaaac cttctcttcg
     2041 taatcgacca agaagtcacc cgacttgttc aacggagctt cgatcttcgg catgtgcgtc
     2101 tccaaagtga gcggagcggc gagctcgccg cgcggcgcga accgttttgc gagacgcatg
     2161 ccacgcgcaa ttcatgcgcc aaatcgagct atttgcgcat tgaacgcggg gcagcgcgct
     2221 ctctccagaa acacaccgaa tcctgcgact cgcgaatcct cgagagcagg ctcgcaattt
     2281 cctgcgagct acgagtctgg gagatttcgg cgcgctcgcg tgcacgcgac cgaaacgttc
     2341 cgccgctatc gcggtcgctc gaggccgagc tgcttcatgc ggtacgcgag cgtgctcgga
     2401 ctgatgccga ggagcttcgc cgcgccggcc gcgccggaga tctggtagcc acagcgctcg
     2461 agcgcacgct gcagattttt ccgttcgaac tcacgcatga catgttccgg caacacgtcg
     2521 tcctgtactg ggcgacgcgc cgactccggt gggggaacgc tcatcggtcg ccccaggccg
     2581 agacgcagga cgccgtcacg ctgcatcaag accaacgcgc gctcgagcac gttctgaagc
     2641 tcgcggacgt tgccgggata gtcgtaggcg agtagcgctg cttcgtcggc tttggagagc
     2701 tcgggaaccg ccaaatgcag gcgatgagcc gtggtttcga ggaaccggcg cgccatcggc
     2761 agaatgtcct cgcggcgctc tcgcaacggg ggcacctcga tgggcagcac gttgagccga
     2821 tagaagagat cacgccgaaa acgacccgcc tcgacttcgg tctccaaggg acgattcgtg
     2881 gccgcgatga cgcgcacgtc gacgcgccga gttcggtcgt caccgacggc ctcgaaggtc
     2941 cgctcctgca ggactcggag cagcttgccc tggagctcga tcgggatctc gccgacctcg
     3001 tcgaggaata gcgagccgcc gtcagcgagc agaaagcgtc cctgccgatc tcgcgcggcc
     3061 ccgctgaagg caccacgcac gtggccgaag aactcgctct cgaagagctc ccgcggcacg
     3121 ctcgcgcagt tcactttgac gagcggcccg tcgcgccgtt gactgaggtc gtgcacggca
     3181 gccgcgatga gctccttgcc cacgccgctc tcacccaaaa tgaggactga ggcgtccgca
     3241 gccgcgaccg cgcgcagctg ttcgagcacg cgacgcatgc ctgggctctc ggcgatgatg
     3301 tcgccgtgct ggagagcgct ccgcaccgct tcgcgcaagt agtctcgctc gcgctcggcg
     3361 ctctccttca agcgctcgat ttcggcaaac gcgcgggcgt tcgcgatcgc cacggccgcg
     3421 tgatcggcga aaacccggag ccaatcgagg tccgacttgc cgagccggcg ccggctgaag
     3481 aatgcaacga ctcccagcac ctggccgcga aagcaaagtg gttgcccggc gaagctctgg
     3541 atcccctccg cctccgccca ctcgggctcc acgatccagt taccggggcc ccgctgcaaa
     3601 agcagcgggc gattgctcgc ggcgatgtga cccaccttgc cgaagctcag cgggatccgt
     3661 cgatgcgacc catcgatgcg gttccagcgc cggtccaggt cgacgatcga ggcgccgatg
     3721 ctcgctttga gttcgaggta ggctccgtcc cgatcgtctt cgcgcagcag ccaaactcgg
     3781 gccagagcta ccccttccca catccccaga ccctgcacca tctcggtcag gactgagtcc
     3841 ggcgaacggg cttgagcgac ggagagcgcg agcgactgga gcgcttcgag ctgcattcca
     3901 ggcccaagca tcggccgcgc gggagcactt cgcgttttcg cgaatgtttc cggtcttcgc
     3961 tcgcactgac ggccagctcg ctgggcggta gtcccggtgt tgcccgctcg cgacgcgcgc
     4021 tcagtccgtg gaagagcatc gcgcgggcac gacgagcgcg ccgtgcccgc gccacgactt
     4081 caatcgggag atgtctccgc tcgcaggttg gagcgaagtc gctcgagcgt gtgctcgatg
     4141 accggcatca actcgcggcg cgtcgactcg gcaactcgcg ctgcggtttg gtggcgcact
     4201 tcatctgcgc cggtgccgtc gatctcttcg atcgccgtga tcaccgactc ggcttccttc
     4261 tcgagcgcac ggagcttcgt ctctcgacgc tcggggtcga gctgccgtgc agcacgcaag
     4321 aggagcccga gagtgtctac cagctcgtcg agatcatgtc cgggagctgc tcgcttcggt
     4381 acggcatcct cgggcacggc gccgccagtt ggggccaccg cgccgggcgc ggagtccacg
     4441 gcccgctcga gttgctcgtc ttgtcgattc ttcgaggtgt gcacatttcc ctcctggcca
     4501 gggcttcgac tggcgcgctc aatgcgccag tcgctcacga aaatgttcga gcgactgctt
     4561 cagatcgcga gccaaagtgg cggtcgcgtc catcaaggag ccgccgtccg ccgaaacgcg
     4621 gtgttccagg ttttgtacct gctcctccaa cgcttgccac ttctctttgg cgtcgaggcc
     4681 cgcgagatgc accttgaggc gaatttcgtc tcgcagctgt gcgatggatt cgatgacgtt
     4741 cgagtgcgaa ttgccagatg agttcgtggt catgacttcc cgaattgcat agatcgcgcc
     4801 acccggttgc cagtggaaat acaggcattt cgcgcgcaac taccgtcaaa atcgtcgcgc
     4861 acggggcgat ttagtgcgcg gccggagctc tcggcgctaa tgatgcgcgg caacggggat
     4921 gacgagcatc cacggcacgt ccctggtccg agtcgcgctt ttcactgaca cgctttcagc
     4981 tcaaccgaag ggcaagcacg cgcgtactcg gcgcacgttc tccgccagag caagctccgt
     5041 atcgagcacg acgtgctccg aagggccaag ctcttcgatg gggtcgaagc ttcgcgcaaa
     5101 gtcatcgaag atttcgatgc ggccatccga aacgctccgg ccctccgcgc gcttctgcag
     5161 tcgcgcgagg cacacctcac ggctcgccct gcattcgacc agcacgaaag cagcgccaac
     5221 tcgctgcgcg agctcgcgca cggccaacct ttcggagcga ctgcgaaacg atgcgtcgag
     5281 gatcacggag cgacccgatg ccaggacggt agtcgcgccc tccctgagct cttcgtaaac
     5341 ccgtcgcgtc gcttcgctcg agtagttcga agcgaacgag gggtccgaaa gcggcgtctc
     5401 ggcagaaacc ccggcgtgcg ccttgcggac gcgatccgcg acgatcaccg gtagcgcgag
     5461 ctccgccgca agcgcctccg cgacgtggct cttgccagac gcgatcacgc cgcacacgac
     5521 gatgagcgcc gtgcgcgcca gcggacgcct cgccgcggag agcgccaaca agtagtgccg
     5581 gcgagcctcc gcatcgctgc gctctcgggt catcacgtcc acgtcgggcg atagggcttg
     5641 aaagcccgca accttcgctc gcacgtgcgc gcggtagctc tgatagaagt ctacgagcgc
     5701 gtaaagaccg aagtcgttcg acaagagcgc gtagcgggcg agcagtcgct ccgcgagatc
     5761 cacgcggcca tgctccgcca agtccatcga gaggaaggcc acgtcggcgc atacgtcggc
     5821 aaagcggaag cgctcgttga actcgatgca atcgatgatg gcgacgccgt cgccgagcat
     5881 gtagacgtgc tcgagccgca agtccccgtg accgtcacgt attcgcccct cgttcatgcg
     5941 ccgggtgaag agctcggcgc gctctcgaag aaagtccttt tgaaagcgct cgagctccgc
     6001 tgcctgttcc ggctcgaggt aacgcccgat cgcttcgcga gtctgtgcga agttctcctc
     6061 cacgttcccg cgcacggcct ccacgctgcc ccagtgcctg cgctcgggct cggcggagag
     6121 ctccgcgtgg aagtcggcga gccgctgcgc gacatcgtcc aactcgcggt gctcgaaagc
     6181 accggccacg agcagccgat cggcgcgtcg ttcgtccggt aggcgcttca tgtgcacggc
     6241 gtagtcgacg agctctccgc cgcccccgag ctcgtggtga ccgcccgcga ctcgcgtcac
     6301 ggagaccagg ccgaggtata cgccttcggc caaacgccga ttcagcaaca cctcggcttc
     6361 acaggcgcgg cgccggagct ccagcgtcgt gaagtccaga aagccgaaat cgaccggctt
     6421 cttcaacttg aagacgtgtt ccccgatcaa gaagacgcgc gagatgtggg tctcgatgag
     6481 ggtcacgggc cgctcgcgcg cgtctccgcc gagcacgggg aggtacgctt cccagcgcct
     6541 caggtcggag agcaacacgt cggcgtccat gtccgcgcgc agtatggccc cctgcggata
     6601 ccgcggctag cgcttcgcgc aaaggctgcg gcgaaacgca atggctgcac tcgccacgac
     6661 cgattggcgc gcggaagtcg cacggaaaca cagcaccgac accgacgccc gtatggcacg
     6721 ggcagtgcac ttaactaatt caacgaagga gtgatcatgg acaagccctc gaagccgttc
     6781 gttgcggtag ttggatacga cttttctgag ctcgccgacc tcgcgctcga ccaatgtctc
     6841 gacgtcgtgt cacggcatcc agaagccgag gttcacgtcg tctacgtcgt gccagccgta
     6901 gcgctaactg caaatcctct cgctgctgcg ctcccttctg cggtgagcgg tgacgtactc
     6961 gacgatacgg cagcgcgctt gaaaaaggcc gtcgacgacc gtctcgcgca ctacgtaaag
     7021 actcatcagc cgctcacctg ccgcgttgta tcacacctca gggtggacga aacagcatcg
     7081 tgcatcgcgc agctggctgc cgatctggaa agcgacatct tggttgtcgg aactcatagc
     7141 cgtcgcggcg ttgcgcggct gctgctcggc tctgtcgccc agtcggtgat cgccctcgcg
     7201 ccctgccccg ttctcgtcgt acgccccaag cgcatcgagg cgcacagccc cgcgatcgag
     7261 ccaccttgcc cgcgttgcgt cgaaacgcgg caagctacgc aaggcgccga gttctggtgc
     7321 gagcaacatc gcgaacgcca tggtcgtcgt cacacctacc atcaggacga tcgcacctcg
     7381 agcgactcca cgcttcccct agtagtgcac tgaaggagac cacggagaga ccgcggacgc
     7441 agagcatgca cgctcccgca aatcctgcgc gcgtcgtgga acccgaagga aagaacctgg
     7501 gctttcacgc cgcgcgcagc gtcgtttcag gggcggcatg cgtcttgcca agagagcgcc
     7561 tcggatgacc acccacgtcg aaaagttctc ctacgacgac gccaccgttc gcaagttcgc
     7621 gtttgcaaca gcgatttggg gcgtcgtcgc tactctggcc ggtctctggc tcgcggtgat
     7681 gctcgtggcg ccatcgctga gctttggact tccacagctc accttcgggc gcctgcgccc
     7741 gttgcacacg aacgctgcga tcttcgcctt cgccggcaac gcggtgtttg ccgcggttta
     7801 ctactcgagt cagcgcctgc tcaaagcgcg gatgttcagc gacgcgctca gcaagttcca
     7861 cttctggggc tggcaagcca tcatcgtcgc tgcggcgttg acgctgccct tcggcatcac
     7921 tcaatccaag gagtacgcgg agctagagtg gccgatcgac ctcgccatcg cggtggtgtg
     7981 ggtcgccttc ggcatcaact tcttcggcac cgtcgccaag cgacgtgagc ggcacatcta
     8041 cgtcgcgatt tggttctaca tggcgacagt cgtcacggtc gccattctgc acatcttcaa
     8101 caacatctcg atccccgcgg gggcgttgaa gagctattcg gtctacgccg gcgtgcagga
     8161 cgccttcatg cagtggtggt acgggcacaa cgccgtcgcg ttcttcctga cgacgccgtt
     8221 cttgggcttg atgtactact tcctgcccaa ggccgcgaac cgccccgtct tctcgtatcg
     8281 gctttcgatc ctccacttct ggtcactcgt cttcatctac atctgggcgg gaccgcacca
     8341 cctccactac acggcgctcc cggagtgggc ttcgacgctc ggcatgttgt tcagcgtgat
     8401 gctctggatg ccgtcgtggg gcggcatgat caacggcctg ctcacgttgc gcggcgcttg
     8461 gaaccgcgtc tccgacgacc cggtgctcaa gttcttcgtg gtcggcatca cgttctacgg
     8521 catgtccacc ttcgaaggcc cgatgctttc gatcaagagc gtgaacgcgc tctcgcacta
     8581 caccgactgg acgatcgcgc acgtgcacag cggcgcgctc ggctggaacg gcttcatgac
     8641 gttcggcatg ctctattggc tcgcgccgcg catcttccag acgaagctct ggtccaagaa
     8701 gctcgccgag gttcacttct ggctcggaac gctcggaatc ctgctctaca tcgtggccat
     8761 ctacgccgcg ggcgtgaccc agggcttgat gtggcgcgcg ttcgatcaga ccggccgcct
     8821 cgcttacccg gacttcgtcg agacggtcgt gcgcatcctg ccgatgtatt gggtgcgcgc
     8881 gctgggcggt ctgctcttcg tcacgggcgt ggtgctcggg ctcgtcaaca tcgtgatgac
     8941 ctggaaaacg cggccggcca cctacaccga gccgaccgac gaggctcccg cgctcacccc
     9001 gtacacgggc gagacgactc gcttcgtcat cccacgccac agcacgatct cgaacttcgg
     9061 ccacgccgtg cgctacctgg ccgaagctcg ctggcaccgg gtctgggagc gcaagccgtt
     9121 gaagttcgcg ctgtgggtcg ccgcgtcggt gatcatcgcg tcgctcttcg agatcatccc
     9181 gaccttcctc atccgctcga acgttcccac catcgcttcg gtgaagcctt actctccgct
     9241 cgagctcctg ggtcgtgaca tctacgtgag cgagggttgc tacaactgcc actcgcagat
     9301 ggttcgccca atccgggccg aaaccgagcg ctacggcgag tattcgaagc cgggcgagtt
     9361 cgtctacgac cacccgttcc agtggggctc tcgccgcatc gggcccgacc tacatcgcgt
     9421 cggcggcaag tacccgcacc tctggcacgt gcgccacatg caggacccgc gctcgacgac
     9481 gcctcaatcg atcatgcccg cttatccctg gctcgcctcg aacgatctcg atttcagcgt
     9541 cgtccagaag cgcgtcgacg tgatggcgat gctcggcgtt ccgtatggcg acgcagtcaa
     9601 ccacgccgaa gagctcgcgc aggcgcaagc gcaccgcatc gcgtccgaaa tcgaggccca
     9661 gggcggaccg cgcggcctcg aacagaagca aatcatcgcg ctcgtcgcgt atctgcaacg
     9721 gctcggcacc gacatcaagg cgccggtcaa cggcgacaag ccagctcgca tcgcccggag
     9781 cgaaggcgcg gcaaccggag aagtcatcaa atgaaactca gtgacatcgt ctcggctcta
     9841 cacttgtcca tcttcgcgga agttccgctg atcatctgct tcggcatctt cctcggagtc
     9901 gtgctgccag tcatgcgttc gcgcgaagag ttcgagcaaa tgcgaaccct cccgctccgc
     9961 aacgacgaac ccgagaccga ccctacgcgt cgtcaaggag ggtcgccgtg aacgagcccg
    10021 tttcgacctc gagccgcccg cccagcaagg cgccgcccgc ggaagagcac cgcgccgaca
    10081 ccgcctccgt gctcgatcac gagtacgacg gcatccgcga gtacgacaac ccgctcccac
    10141 gctggtgggt caacatcttc tggggctcgt tcgtcttcgc gattgggtac ttcttccact
//
LOCUS       DASTKS010000124         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1581005, whole genome shotgun sequence.
ACCESSION   DASTKS010000124 DASTKS010000000
VERSION     DASTKS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581005"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFQ35_02665"
     CDS             <1..270
                     /locus_tag="VFQ35_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929556.1"
                     /translation="YLGGDVVGYWAPHFRDTDEYETLVQPRAYFGVRILKPLSVFAGA
                     SYNVLLASRRANHWAPDYAQRVDGTGEPYVRLWPGAIVGVRLLPE"
     gene            313..1863
                     /locus_tag="VFQ35_02670"
     CDS             313..1863
                     /locus_tag="VFQ35_02670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9929557.1"
                     /translation="MRFEELAIASREIGDTRSRLKKIERLSVLFTALAPIEIEPAVAL
                     LSGEPRQGRIGVGGALLSRLFSQPTSSTASVSVEEVDAALQRFSELNGPGSARERELV
                     LGALFSRLTELERAFLAGVLGGGLRQGALEGVVLEAVAKAARVPMEALRRALLFAGNV
                     HVVAREALTNGGAGLGGFALTLFQPLRPMLADTAPTLAEALEQEPRAALEYKLDGARI
                     QLHKRGQDIAVFSRHGQDVTARVPEIVASAREFPANELLLDGEAIAFAANGRPLPFQT
                     TMQRFGRQKNVETARESIPLSQVYFDCLYKDGKLLVDRPNEERFAALRDSIAAPLVVP
                     RQVVDDAKLAERFVSSALEAGHEGVLVKSLRASYDAGRRGSTWLKLKPVHTLDLVVLA
                     AEWGSGRRKGYLSNIHLGARDPVGGGFVMLGKTFKGMTDAMLQWQTKRFQELVEEPGE
                     YVVKLRPEVVVEIAFDGVQESSQYEGGLSLRFARVKSYREDKRPEEADTIARVREIFE
                     RSRGVVTA"
     gene            complement(1952..>2368)
                     /locus_tag="VFQ35_02675"
     CDS             complement(1952..>2368)
                     /locus_tag="VFQ35_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9929558.1"
                     /translation="PTSPGAERSFTPEQLGEALALADHVVSLLPGDASTQGFFGASAF
                     SKMKPSAYFYNLGRGSTVDEAALVTALGNGTIAGAFLDVTAEEPLRPESVLWRTENLY
                     ITPHSSAIAAQYLDLYFDELAPMLRAAAKGTPSIAP"
BASE COUNT          378 a          741 c          829 g          420 t
ORIGIN      
        1 tacctgggcg gcgacgtggt cggctactgg gcgccgcact ttcgcgacac cgacgagtac
       61 gagacgctgg ttcagccgcg cgcctatttc ggcgtgcgga tcttgaaacc gctgtcggtg
      121 ttcgctgggg catcttacaa cgtgctgctc gcgagccggc gcgcgaacca ctgggcgccg
      181 gattatgccc aacgcgtcga cggcaccggg gagccctacg tgcggttgtg gcctggcgcg
      241 atcgtcggtg tgcgattgct tccagagtga cccgcttgac ggggcagcac atgcgtccca
      301 acctgtgcct cgatgcgctt cgaggagctt gcgatcgcgt ctcgcgagat cggcgacacc
      361 cggtcgcgcc tgaagaagat cgagcggctc tcggtattgt tcacggctct cgctccgatc
      421 gagatcgaac cggccgtcgc gttgctctcc ggtgagccgc gacaaggccg catcggggtc
      481 gggggcgctc tgttgagccg gctgttttct cagccgacct cgagcaccgc gagcgtttcg
      541 gtcgaagagg tagacgcggc cctgcaacgc ttttcggagc tcaatggacc gggttcggcg
      601 cgtgagcgcg agctcgtgct gggcgccttg ttctcgcggc tcaccgagct cgagcgcgcg
      661 ttcttggccg gcgtgctcgg cggcgggttg cggcaaggcg cactggaagg ggtcgtgctc
      721 gaggcggtcg ccaaagcggc gcgcgtgccg atggaggcgc tgcgtcgcgc gctgctcttc
      781 gcgggcaacg tgcacgtggt tgctcgcgag gcgctgacca acggcggcgc ggggctcggc
      841 ggctttgccc tgaccttgtt tcagccgctg cgcccgatgc tcgccgacac tgccccgacg
      901 ctcgccgaag cgctggagca agagccgcgc gctgcgctcg agtacaagct cgacggcgcg
      961 cgcattcagc tccacaagcg cgggcaagac atcgcggtgt tctcgcgcca cggtcaggac
     1021 gtgacggcgc gcgtgcccga aatcgtggcg agtgcgaggg aattccccgc caacgagctc
     1081 ctgctcgacg gcgaggcgat agcattcgcg gcgaacggaa ggccgctccc gttccaaacg
     1141 acgatgcagc ggttcggtcg gcagaagaac gtggagaccg cgcgcgagag catccccctc
     1201 tcgcaagtgt acttcgattg tctctacaag gacgggaagc tactcgtcga tcggccgaac
     1261 gaagagcgct tcgccgcgct gcgcgacagc atcgcggcgc cgctcgtggt gccgcgccag
     1321 gtggtcgacg acgcgaagct cgccgagcgc ttcgtttcga gcgcgctcga agccgggcac
     1381 gaaggcgtac tcgtgaagag cttgcgcgct tcttacgacg ctggtcgtcg cggctcgact
     1441 tggctcaaat tgaagccggt gcacacgctc gacctcgtcg ttctcgccgc cgagtggggc
     1501 agcggcaggc gcaagggcta cttgagcaac atccatctcg gcgcgcgcga cccggttggt
     1561 ggcggcttcg tcatgctcgg taagaccttc aagggcatga ccgacgcgat gctgcagtgg
     1621 cagaccaagc gcttccaaga gctcgtcgag gagccgggcg aatacgtcgt gaagctacgt
     1681 ccggaagtgg tggtggagat agctttcgac ggagtgcaag agagctctca gtacgaaggt
     1741 gggctttcgc tccgctttgc gcgcgtaaag agctaccgcg aggacaagcg ccccgaagag
     1801 gccgacacca tcgcgcgtgt acgcgaaatc ttcgagcgaa gtcgtggtgt cgtcacggcg
     1861 tgaagctcgg tgcgcgccct gacttcgtcg cggatcggca tgcgcgtgtc ggtcgcggcg
     1921 gcgtggcgcg cggcatttgg cgatggcgtc tttacggcgc gatgctcggg gtgcctttgg
     1981 cggccgcgcg taacatcggg gcaagctcgt cgaagtacaa gtccaagtat tgcgccgcga
     2041 tcgccgacga gtgcggcgtg atgtagaggt tttcggtacg ccacaacacc gactcgggcc
     2101 gcaaaggctc ctcggcggtc acgtccaaga aggcgccggc gatggtcccg ttcccgaggg
     2161 ccgtaacgag cgcggcctcg tccacggtgc tgcctcgtcc gaggttgtag aagtacgcgc
     2221 tgggcttcat tttcgagaac gccgacgctc cgaaaaatcc ctgcgtgctc gcgtcgccgg
     2281 gcaacagcga aaccacgtga tccgcgagcg ccaaagcttc gccgagttgc tcgggcgtga
     2341 aactacgttc tgctccgggg cttgtggg
//
LOCUS       DASTKS010000125         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_689366, whole genome shotgun sequence.
ACCESSION   DASTKS010000125 DASTKS010000000
VERSION     DASTKS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689366"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..>3323
                     /locus_tag="VFQ35_02680"
     CDS             330..>3323
                     /locus_tag="VFQ35_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HET9929559.1"
                     /translation="MPCADNSVVPPRSSRSGRLRGVRTAIALFAALATTTANVKQPLA
                     KGVVHPDLDTALAELDRAKGAESYAALRRVWDTWDRADPAQVEEALILASKSNRLSAS
                     AQTYAATLAAYARLRRGDLKAAHDELHALGYIDHWLAVGPFDNDGKAGLAAESGPELD
                     LAQPLSTSKAYTGRERAVRWRALPDAFSYGFLDFGSVVRPESKVCAFAASFVKATGEK
                     PAARDVSVWVGSGGAFRLFWNGREVLESDIYSRHDFERMAARVRLEPGPNLLTLKVCG
                     DDQAPTISVRVGDAKGAPDPTLSFSNDVADAEAAATLVAQLAKSKAPAKAAATGLEGP
                     VQYFTKLTSKANASSAALEAHARYLDETDGDDPAQHLARDLARRAAEREPSVERFLLA
                     GRLAEDRNKAGEWIEKAEKLTRHKPHRDVLLARAWQRRHGPNYREALPYFDGALRLDP
                     TNVEALRGITELYDLAGLPRTALARVEKALEQNPNSVGLLALYAAQLRTLGRTSDAAD
                     VEARYHGLRFDDSSYVNSELELALSRRDKVGATHWAERLTATQPHDIWALGIAARAYQ
                     RLGEPERAVATHRQALALAPEDVGALRALADLQGDLGHRGEQLSLLQEILRVRPQAKD
                     VREYVENLEPEKPRADEAYAWSKEKFLYLRHAPPAGENRRVLRDLNVTTVFENGLSSQ
                     FHQVVFQPLTDAAAAQDRQYAFGYEADRQVVQLRGAKVYRKNGKVDEAIEWGEGPADD
                     PTIAMYTSARTFYVQLPRLDAGDVVELRYRIDDVTPRNEFKDYFGDVEYMGGRDPLQN
                     SEYVLITPNSRKIFVDAHVPGLEQSTQKGERATIHRFFVKQAPRIQPEPAMPPPSEVL
                     PFIHVSTYENWKDLGRWYWGLVKDQFDLDDETRQLAHKVVNGKKTELEKVQAIYDWVT
                     RNTRYVALEFGIYGHKPRRCVQTVARGWGDCKDKATVIVSLLQELGIDATIVILRTQM
                     RGRFDSKVASLAPFDHAIAYVPSLNL"
BASE COUNT          587 a         1112 c         1094 g          530 t
ORIGIN      
        1 ttgtgcgcgg ccgacgtcgc ttcgctcctc gacagccggg tttgtgcgcg gccgacgtcg
       61 cttcgctcct cgacagccgg gtttgtgcgc ggccgacgtc gcttcgctcc tcgacagccg
      121 ggtttgtgtg cggccgacgt cgcttcgctc ctcgacagcc gggtttgtgc gcggccgacg
      181 tcgcttcgct ccttgacagc cgggtttgtg cgcggccgac atcgcttcgc tcctcggccg
      241 actggctcag agtgtcgcgc ggataggtgc gcacggagtg tgcacttgcg ccgcgtgccg
      301 ccaaaagcta cccgatgacg gtgaaccgca tgccgtgcgc ggacaattcg gtcgttcccc
      361 cgcgctcctc acgtagcggt cgtcttcgcg gagtacggac agccatcgcg cttttcgccg
      421 ccctcgcgac gaccaccgct aacgtcaaac agccgctcgc caagggcgtc gtgcaccccg
      481 acctcgatac cgcgctcgcc gagctcgatc gagcaaaggg cgctgagtcc tacgcggcgc
      541 tgagacgcgt gtgggacacc tgggatcgag ccgatccggc gcaggtggaa gaggcgctga
      601 tactggcttc gaagtcgaat cgtctcagcg cgagcgcgca gacctacgca gcgacgctcg
      661 cggcgtacgc acgcctgcga cgcggcgact tgaaggcagc tcacgacgag ttgcatgcgc
      721 tcggctacat cgaccactgg ctcgcggtcg gtcccttcga caacgacggc aaggccgggc
      781 tcgccgcaga gtcgggtccg gagctcgatc tcgctcaacc gctatccact tcgaaggcct
      841 acaccgggcg tgagcgcgcc gtgcgctggc gcgccttgcc cgacgccttt tcttacggtt
      901 tcctggattt cggctcggtc gtgcgccccg aaagcaaggt gtgcgctttc gccgcgagct
      961 tcgtcaaagc aaccggggag aaacctgccg cgcgcgacgt gagcgtttgg gtcggtagcg
     1021 gcggcgcgtt tcgattgttt tggaacggcc gcgaagtact cgagtccgac atttattcgc
     1081 gccacgattt cgagcgcatg gcggccaggg ttcggctcga gccggggccg aacttgctca
     1141 cgctgaaggt gtgcggcgac gatcaggcgc cgaccatatc ggtgcgcgtg ggcgacgcaa
     1201 aaggcgcgcc ggatccgacg ctttcgtttt cgaacgacgt ggcagacgcc gaggccgcgg
     1261 cgacactggt ggcgcagctc gcgaaaagca aggctccggc gaaggccgcg gcgacgggtc
     1321 tcgaaggtcc cgttcagtac ttcacgaagc tcacctccaa ggcgaacgct tcgagcgctg
     1381 ccctggaagc gcacgcgcgc tacctcgacg aaaccgacgg tgacgatccg gcgcagcacc
     1441 tggcccgcga cttggctcga cgcgccgccg agcgcgagcc ctcggtcgag cgcttcttgc
     1501 tcgcggggcg actcgccgag gaccgcaaca aggcgggcga gtggatcgaa aaggccgaaa
     1561 agctgacgcg ccacaagccg caccgcgacg tgctcttggc gcgcgcctgg cagcgccgcc
     1621 acggccccaa ctatcgagaa gcactgccct atttcgacgg tgcgctccgg ctcgatccga
     1681 ccaacgtcga agcgctccga ggcatcaccg agctctatga tttggcgggt ctgccgcgca
     1741 cggcgctcgc gcgcgtcgag aaagcactcg agcaaaaccc gaactcggtg gggctgctcg
     1801 cgctgtacgc tgcgcagctc aggaccctcg gtcgcacgag cgacgccgcg gacgtcgagg
     1861 cgcgctatca cgggctgcgt ttcgacgaca gcagctacgt caacagcgag ctcgagctcg
     1921 ccctgagccg acgcgacaaa gtcggggcga ctcactgggc cgaacggctc accgcgacgc
     1981 agccccacga catttgggcg ctcggcatcg ccgcgcgggc ctatcagcga ctcggcgagc
     2041 cggagcgcgc cgtcgcaacg catcgccagg cgctcgccct cgcgcccgaa gacgtcggtg
     2101 cgctgcgagc gctcgctgat ctgcaaggcg acctcggtca ccgcggagag cagctgagcc
     2161 tgttgcagga aatcttgcga gtgcgcccgc aagccaaaga cgtgcgcgaa tacgtcgaga
     2221 acctggagcc ggagaagccg cgcgccgacg aggcgtatgc gtggagcaaa gagaagtttt
     2281 tgtacctgcg ccacgcgccg ccggcgggag aaaaccggcg cgtgctgcgc gacctgaacg
     2341 tgaccaccgt gttcgaaaac ggcctgtcgt cacagttcca ccaggtcgta ttccaaccgc
     2401 tgaccgacgc ggcggctgcg caagatcggc agtacgcctt cggttacgag gcggatcgcc
     2461 aagtcgtgca gctgcgcggc gccaaggttt atcgcaagaa cggcaaggtc gacgaagcca
     2521 tcgagtgggg cgaaggaccc gcggacgatc cgacgatcgc gatgtacacc tcggcgcgga
     2581 ccttctacgt gcagctgccg cgcctcgacg ccggtgacgt ggtggagctc cgctatcgca
     2641 tcgacgacgt cacgccccgc aacgagttca aggactactt cggcgacgtc gagtacatgg
     2701 gcggtcgcga cccgcttcag aactccgagt acgtgctgat aaccccgaac tcccgcaaga
     2761 tcttcgtgga cgcgcacgtg cccggcctcg agcagagcac ccaaaagggc gagcgcgcga
     2821 ccattcaccg cttcttcgtg aagcaggcgc cgcgcattca gccggagccg gcgatgcctc
     2881 cgccgagcga ggtgctgccc ttcatccacg tctcgactta cgaaaactgg aaggacctcg
     2941 gacgctggta ctggggcctc gtgaaagatc agttcgatct cgacgacgag acgcgccagc
     3001 tcgcgcacaa ggtcgtgaac ggcaagaaga ccgagctcga gaaggttcag gccatctacg
     3061 actgggtcac gcgaaacacg cgctacgtcg cgctcgagtt cgggatctac ggacacaagc
     3121 cgcggcgctg cgtgcagacg gtggcgcgcg gctggggtga ctgcaaggac aaagcgacgg
     3181 tgatcgtctc gctgcttcag gagctgggta tcgacgccac gatcgtgatt ctgcgcaccc
     3241 agatgcgcgg tcgcttcgac tccaaggtgg cgagtctcgc gccgttcgac cacgcgatcg
     3301 cgtacgtgcc aagtctcaat ctg
//
LOCUS       DASTKS010000126         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1804710, whole genome shotgun sequence.
ACCESSION   DASTKS010000126 DASTKS010000000
VERSION     DASTKS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804710"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1159
                     /locus_tag="VFQ35_02685"
     CDS             155..1159
                     /locus_tag="VFQ35_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9929560.1"
                     /translation="MRNFDGGLARLGAYPRYSLCAWPTPVERHELRNGPAIWVKRDDL
                     SGWGRGGAKARKVEHLLGHLLAHGYTDLVTVAGNVTNLAFDLLPALDRAGIAGHLHII
                     DDPPVTAAERAQIFSGIAERVTLLGPSRREAFRRTWQRWFELRRQGRKPLWVMPGVSH
                     PAGILGNAAGYLELVEQLELAGHALPTTVFITAATGTTVAGFLMAERLRRQRGGRPIR
                     IVGVQIYEGRTRAATRWLMRWASLSTGQRLPFAPPVEIASDALSGGFGRFTPELAELC
                     ERVQSETGLLIDPIFGGKTWASMERESARRKPRDGEFLYWHCGYTPEWRTLGKMVNAT
                     "
     gene            1156..2652
                     /locus_tag="VFQ35_02690"
     CDS             1156..2652
                     /locus_tag="VFQ35_02690"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET9929561.1"
                     /translation="MSADHALSIPKAALRPVRSIVRSAIIVIAYVLGFFGVLPITLVR
                     FGLRVDAALGLPEVSGAVGRWLGAPLSLLGWVLLSRAMLELSQRGRGLPISHLPPRRL
                     VVSGWFARLRHPIYVGYSLSLLGAGLALRSVGVALVATPLLVGAWCAYAAFIEEPVLV
                     RRFGASYEAHLARTQRLPHPGELEGLAALLWRLLRPAAEFVAARPVLFRLGPGVWVGY
                     GAFVALGAASAAALAVVFFAKALPSLLVYEYVLGLALCMLVGGRLAWLGYERRALFTA
                     PKETLRRVGFVSFGAYAAMLVFASAWPKLRASELGSLWFLDRTMLACLVCSGFGRLGC
                     LSYGCCYGKPWSHGMRCTHPKSKVRRERGPDGALPRAPTQVLSALLAFGSAALLAALL
                     VQGAPRGFSAAFTAVVYGLSRFGLEGLRDEPRFFDGRLTRGQILAALIGVSALALLAF
                     APFGSRTGLEFVTSLDPSALRAFPWLPALAALPVFTVCGYHRRDVGRW"
BASE COUNT          356 a          904 c          904 g          533 t
ORIGIN      
        1 gatcctcgtt tccgcgtccc cgacgcgttc gtagcgaaga tcctgtaaac gacgctgcac
       61 caggatgtga gcagcggact gcacacatcg cagtttcgcg ccgcgaaacg tccgctgaac
      121 ctaggcggca cggttttggc agccgcgttc gtcgatgcgc aatttcgacg gagggttggc
      181 tcgcctcggc gcctacccgc gctattcgct gtgtgcctgg ccaacgccgg tcgagcgcca
      241 cgagctccgc aacggtcccg ccatttgggt caagcgcgac gacctctcgg gctggggccg
      301 tggcggagca aaggctcgca aggtcgaaca cctgctcggg cacctgctgg ctcacggtta
      361 taccgatctc gtcacggtcg caggcaacgt aacgaacctc gccttcgatc tgctccccgc
      421 gctcgatcgc gccggcatcg ctgggcatct tcacatcatc gatgatccac cggtgacagc
      481 cgccgagcgc gcgcaaatct tctctgggat tgcggaacgc gtcacgttgc tcggaccgag
      541 ccgccgcgag gcgtttcgac gcacttggca gcggtggttc gagctccggc gccaaggcag
      601 aaaaccgctt tgggtcatgc cgggggtgag ccacccggca ggcatcttgg ggaacgccgc
      661 cggttacctc gagctcgtgg agcagctcga gctcgcgggg cacgcgctgc ccacgaccgt
      721 gttcatcacg gccgcgacgg gcacgacggt ggccggattt ctgatggcag agcggctgcg
      781 acgacagcgc ggcggtcggc caatccgaat cgtcggcgtt cagatttacg aagggcgcac
      841 gcgcgccgcg acacgatggc tgatgcgctg ggcctcgctg agcacgggcc aacgcttgcc
      901 cttcgcgccg cccgtcgaga tcgcgagcga cgctttgagc ggtggctttg gcaggttcac
      961 gcccgagctc gccgagctct gcgagcgagt ccagagtgaa accgggctct tgatcgaccc
     1021 gatcttcggc gggaaaacgt gggcgtcgat ggagcgcgag agcgctcgca gaaagccgcg
     1081 cgacggcgag ttcttgtatt ggcactgcgg ctacaccccc gagtggcgca ctctcggaaa
     1141 gatggtgaac gccacatgag cgccgatcat gcattgtcga taccaaaggc cgctctccga
     1201 ccggttcgct cgatcgtgcg ctcggcgatc atcgtgatcg cgtacgtgct gggcttcttc
     1261 ggagtcttgc ccatcaccct cgttcgcttt ggcctgcgcg tcgacgcagc gctcgggctg
     1321 cccgaagtct cgggagcggt ggggcgctgg ctcggcgctc cgctttcgtt gctcggctgg
     1381 gtcctgctgt cccgagcgat gctcgagctc tcgcaacggg gtcgagggct accgatttcg
     1441 catctgccgc cccggcgttt ggtcgtttcg gggtggttcg cgcggctgcg tcaccccatc
     1501 tacgtgggct actcgctttc gctgctcggc gctgggctcg cgctgcgatc cgtcggtgtg
     1561 gcgctcgttg cgacgccgct gctggtcggt gcttggtgcg cgtacgccgc gttcatcgaa
     1621 gagccggtgc tagtgcgtcg gtttggcgcg agctacgaag cgcatctcgc ccgcacccaa
     1681 cgcttgccgc acccaggaga actcgagggc ctcgcggccc tgctgtggag acttctgcgc
     1741 cccgcggccg agttcgtcgc ggcacgaccc gtgttgttca ggctcggacc gggcgtttgg
     1801 gtgggatacg gcgccttcgt cgcgctcggc gccgcttccg ccgcggccct cgcggtggtg
     1861 ttcttcgcga aagcgctgcc ctcgttgctc gtgtacgaat acgtgttggg cttggcgctc
     1921 tgcatgttgg tcggcgggcg attggcctgg ctcggctacg agcgacgcgc cctcttcacc
     1981 gcacccaagg aaacgttgcg ccgggtaggg ttcgtttcgt tcggcgccta cgcggcgatg
     2041 ctcgtgttcg cgtcggcttg gcccaaactc cgggcgagcg agctcggttc gctatggttc
     2101 ctggaccgca ccatgctcgc gtgcctcgtg tgttccgggt tcgggcgact cggctgcctc
     2161 agctacggat gctgttacgg gaaaccctgg tcccacggca tgcgctgcac gcacccaaag
     2221 tccaaagtcc ggcgcgagcg cggccccgac ggcgcgctcc ctcgcgctcc gacccaggtg
     2281 ttgtccgcgc tgctcgcatt cggcagtgct gcgctgctcg cggcgttgct agtacagggc
     2341 gcgccgcgcg gattttccgc tgcgttcacc gccgttgtct acggcctttc gcgtttcggg
     2401 ctcgaagggc tgcgcgacga gccacgcttc ttcgacgggc gactgacgcg cgggcagatc
     2461 ctcgcagccc tcatcggcgt gagcgcgctc gcgttgctcg ccttcgctcc gttcggctcg
     2521 cgcaccgggc tcgagttcgt gaccagcctc gaccccagcg ccctgcgcgc gtttccttgg
     2581 ttgccggcgt tggcggcgct gccggtgttc acggtgtgtg gctaccaccg ccgtgacgtg
     2641 gggcgctggt gaccatgttc tcgtatcgca agctctacgc cgatttgatc accgact
//
LOCUS       DASTKS010000127         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_467487, whole genome shotgun sequence.
ACCESSION   DASTKS010000127 DASTKS010000000
VERSION     DASTKS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467487"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..606
                     /locus_tag="VFQ35_02695"
     CDS             193..606
                     /locus_tag="VFQ35_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET9929562.1"
                     /translation="MTTPHAPKLRILIAKPGLDGHDRGAKVIARALRDAGCEVVYTGL
                     HQTPDMIAAAALQEHVDAVGMSIMSGAHNTLFPAVLAELRTRGLDDVVVFGGGIIPEA
                     DAETLKAQGVRAVFGPGTPLEVIVAWVRDNVRPRI"
     gene            987..1448
                     /gene="mraZ"
                     /locus_tag="VFQ35_02700"
     CDS             987..1448
                     /gene="mraZ"
                     /locus_tag="VFQ35_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9929563.1"
                     /translation="MRWKHMFRGQFLHTVDAKGRVSLPSRFRDVLTAGGDARFVLTPA
                     PFDSCLHLYPMRAWEEFEQKVSELPSMDPNAVRFRRLYVSAAVECELDKAGRVLVPPA
                     LRERAHLERDVLWAGMGRILELWSKADFDRALTMTADEAAQFRAAMEQLKL"
     gene            1451..2383
                     /gene="rsmH"
                     /locus_tag="VFQ35_02705"
     CDS             1451..2383
                     /gene="rsmH"
                     /locus_tag="VFQ35_02705"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9929564.1"
                     /translation="MPATILEEPSFTHVTVMPDEVVQAFAGTTGLVIDATAGGGGHSH
                     ALLSRYPELRLLAFDRDETAVRAASERLSEFGLRARVEHLSFSRIGERLDELGIEEVS
                     GVIADLGVSSQQLALADRGMSFRLEGPLDMRMDTSTGETAAELIERVSQEELADLIYD
                     LGEERGSRRIARCIKQALARGELTTTLDLRRAVVKAIGPRRTGGVDPATRTFQALRIA
                     VNAEIDELSSLLDFSRKRLMDGGVAAFISFHSLEDRRVKREFMKRDFWQRLTPKPIVA
                     SEGEQAQNPRSRSAKLRVGRRHSPLSFDIDAEEE"
BASE COUNT          406 a          784 c          801 g          440 t
ORIGIN      
        1 gtcgacgagc cagtggcctt gctggtaggt cccgagggcg gactcagcga cgctgaactc
       61 gagctcgcgg cggcatccgg gttcgtcgac gcgtcactcg gaccctttac gttgcgcacc
      121 gagctcgctg ccatcgcggc gctcgcgtgc ttcgtcggca aactgccgag cgcatgaagt
      181 agactgagcg cgatgacgac accgcacgcg ccgaagctcc gtattctcat cgcgaagccg
      241 ggactcgacg gtcacgatcg cggcgccaag gtcatcgctc gagcgctgcg cgatgcagga
      301 tgcgaggtgg tctacaccgg gcttcatcaa acgccggaca tgatcgccgc tgcggccctg
      361 caagagcacg tcgacgcggt gggtatgtcg atcatgagcg gcgcgcacaa tacgcttttt
      421 ccagccgtgc tcgccgagct tcggacgcgt ggtctcgatg acgtagtggt gttcggcggt
      481 gggatcattc cggaagcgga cgcggagacg ttgaaagcgc agggcgtgcg cgccgtgttc
      541 ggtcccggca cgccgctcga agtgatcgtg gcgtgggtgc gcgacaacgt gcggcctcgc
      601 atctgagtcg ttgggtccac ggcggcgtgt gcgttcttcc acgccgcgcg ccgcgcaaag
      661 ctaacgaaat tagccagatt tcgagctttt ctcgggagac ccgagctttg caaacagagc
      721 gcgcatggtc accgtcgcgc tgctctcgag tgtgctctcc gttctcttgc tgatcggaac
      781 gctccgcgtg cgcccgggcg tacgtcgttt gcgtgcccca ctttgtccca cgctgaacac
      841 tggatcagtg aaccgcgccc gctaatacga aagttgaagc ggattctcaa gaatttggcg
      901 atccgcattg ttgacatgac ggccccgcgg cccgtaggtt gggccatcca tgtgtgaaac
      961 cgtgggaaaa agtgtcgcgg taacccatgc ggtggaagca catgttccgc ggccaattcc
     1021 tccacaccgt cgacgcgaaa ggacgcgtga gcttgccctc acgttttcgc gacgtgctga
     1081 cggcaggtgg cgacgcgcgc ttcgtgctga cccccgcgcc gttcgacagc tgcctgcatc
     1141 tctatccgat gcgcgcttgg gaggagttcg agcagaaggt ctcggagctg cccagcatgg
     1201 acccgaacgc ggtgcgcttt cggcgcttgt acgtttcggc ggcggtcgaa tgtgagctcg
     1261 acaaagcagg ccgcgtgcta gtgccgcccg ccctgcgtga acgagcacat ctggaacgcg
     1321 acgtcctttg ggccggaatg ggccgaatcc tcgagctctg gtcgaaggcc gacttcgacc
     1381 gtgcgctcac catgaccgcc gacgaggcgg ctcagtttcg cgccgcgatg gagcaactca
     1441 aactatgacc atgcctgcca cgattttgga ggaaccgagc ttcacccacg tcaccgtgat
     1501 gccggacgaa gtcgtccagg cttttgcggg gacgaccgga ctcgtcatcg acgccacggc
     1561 gggcggtggt gggcattcgc acgctttgct ctcgcgttac ccggagctcc ggctcctcgc
     1621 gttcgatcgc gacgagaccg cggtgcgcgc agcgtcggag cgcctctcgg agttcggtct
     1681 gcgtgcccgc gtcgagcacc tgtcgttctc tcgcatcggg gagcggctcg acgagctcgg
     1741 catcgaagag gtgtcgggcg tcatcgccga tctcggcgtg agctctcagc agctcgcgct
     1801 cgcggatcga ggcatgagct ttcgtctcga aggcccgctc gacatgcgca tggacacgtc
     1861 gacgggcgag accgcggccg agctcatcga gcgggtgtca caagaagagc tcgccgacct
     1921 catctacgat ctcggagaag agcgcggctc gcggcgcatc gctcgttgca tcaagcaagc
     1981 gctcgcgcgc ggtgagctca cgaccacgct cgaccttcgc cgcgcggtgg tgaaagccat
     2041 cggcccgcgc cggaccggcg gcgtcgatcc ggcgacgcgc accttccagg cgctgcgcat
     2101 cgccgtgaac gccgaaatcg acgagctctc gtcgctgctc gacttctcgc gcaagcggct
     2161 catggacggt ggcgtcgcgg ctttcatcag ctttcactcg ctcgaggatc gccgggtgaa
     2221 gcgcgagttc atgaagcgcg acttttggca gcgcctgacg ccgaagccca tcgtggcgag
     2281 cgagggcgag caagcgcaga acccgcgctc acgtagcgcg aagctgcggg tggggcgccg
     2341 ccacagcccg ctttccttcg acatcgacgc cgaggaggaa tgaaagacga acgcaccttc
     2401 ctcgtcctgt ggacgctcgc cgtcgcggcc g
//
LOCUS       DASTKS010000128         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1135554, whole genome shotgun sequence.
ACCESSION   DASTKS010000128 DASTKS010000000
VERSION     DASTKS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135554"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..478
                     /locus_tag="VFQ35_02710"
     CDS             341..478
                     /locus_tag="VFQ35_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929565.1"
                     /translation="MPNRQLSTPPWLFLERQPELQAAAFLELRELEQADGRHSEHAAG
                     A"
     gene            complement(512..>2661)
                     /locus_tag="VFQ35_02715"
     CDS             complement(512..>2661)
                     /locus_tag="VFQ35_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9929566.1"
                     /translation="SRGTMAVTMRERGDTVMSVFSRFPERLQHAIANHLGFSSLRPVQ
                     ELAGQAILDRKNAVVLAPTAGGKTEASIFPILAQLLENPPSGVGAIYVAPIKALLNNQ
                     EVRLGDYTQMVGLDRFVWHGDAAQSQKEHFCREPTALLMTTPESLEVMLMSPRVPVQR
                     LFGELQSVVIDEVHAFAGTDRGAHLMCVIERLVALSKHDVQRIGLSATVGNPEHIAEW
                     LRGTSRREAVVIDPPKPKAQRQIKILLREDVSDFAREASRESLGKKSLFFCQSRALTE
                     AVADEMRGESIEVFVHHGSVSKEERRAAEEKFARGTNACIVATSTLELGIDVGGLDLT
                     FQANAPSTVSSFLQRMGRTGRREGTVANMTFLCENAVSVVQNIALVLLASRGWVESVR
                     DETRAWPVLVHQLLALTHSHGGISPERCWELLHRVPDFRGISEQEYRALVEHMKREGY
                     LFEAGGLLSMGQKAERVFGRKNFLELYAVFSSPVLYQVETETGRALGSLEQNFVDRLV
                     EEMSSFLLGGRAWVVLSLNHEERVVRVREAPRGKKPSWGGFVPQFLGFEVCQEMKRVL
                     ATDDDYPFVDTKGKAALAEWRDDLGKLLSSTDSLLADGAQITWWTFAGGRINQTLKYA
                     LEWQNGWKVVPDNFSIRIEGDGVSHETVRAALAKIAVPGFWSNSETRTKLRSLVPNYR
                     LSKFQQALPDAWQTEMLGAYLLDFDGAARVLTA"
BASE COUNT          455 a          881 c          836 g          489 t
ORIGIN      
        1 gccgccgagt cgctgcccgc gggagccacg taatagtccg ccgcctttgc ggcgtgcgcc
       61 caaccgagcg cggctcccac gacgagggcg ctgcgccaac accgctgccc gccgcgacgc
      121 gccgagccac cgtcgttctc gagccacttt tttttcatgc agtttctctg gtaggagccg
      181 cttcgtccca catgtttgca ttgccacgga gctctgggca ccgtctccga aagttgccgc
      241 tctgccgaag acatcgtcac atttcgacct tcgggtccgt cctttgcgcg gtcggtcgac
      301 ttttgcgctt cagccggatt ctttcacgga tcagcacagg gtgcctaatc ggcagctgtc
      361 cacccctcct tggttattcc tcgaacggca gccggagctt caggcggccg cgttcctcga
      421 gctgcgcgag ctcgaacagg cagacggaag gcactccgag cacgccgccg gcgcgtgagg
      481 cgcgtttgac gacgcacctg gcgtcgtcga actaggccgt cagcacccga gccgcgccgt
      541 cgaagtcgag caaataggct ccgagcatct ccgtctgcca cgcgtcgggc agcgcctgct
      601 ggaacttgct gagacgatag ttcggcacga gcgagcgcag cttcgtgcgc gtttcgctat
      661 tggaccaaaa tcccggaacc gcgattttcg ccagcgctgc gcggacggtt tcgtgcgaaa
      721 cgccgtcccc ttcgatgcga atcgagaagt tgtcggggac gaccttccag ccgttctgcc
      781 actcaagggc gtacttgagg gtttggttga tgcggccgcc ggcgaaggtc caccacgtga
      841 tctgcgcgcc gtcagctagc agggagtcag tcgagctgag gagcttgccg agatcatcgc
      901 gccactcggc caatgcggct ttgcccttcg tgtcgacaaa ggggtagtcg tcgtcggtcg
      961 ccagcacgcg cttcatctcc tggcagactt cgaagcctag gaactggggg acaaagccgc
     1021 cccagcttgg tttcttgccg cgcggagcct cgcggactcg gacgacgcgc tcttcgtggt
     1081 tcaaggacag gaccacccag gcgcgaccgc cgaggagaaa agagctcatt tcctcgacca
     1141 gacgatcgac gaagttctgt tcgaggctgc cgagggcgcg ccccgtctct gtttccacct
     1201 ggtagagcac ggggctcgag aagacggcat agagctcgag gaagttcttg cgcccgaaca
     1261 cgcgctcggc cttctgcccc atgctgagta gcccgcccgc ctcgaatagg tagccctcgc
     1321 gcttcatgtg ctcgacgagc gcgcgatact cctgctccga aattccccgg aagtcgggta
     1381 cgcggtgcaa gagctcccaa cagcgttcgg ggctgatgcc gccatgcgag tgcgtgagcg
     1441 cgagaagctg atggaccagc accggccacg cacgggtctc gtctctcacg ctctcgaccc
     1501 agccgcgcga agcgaggagc accagcgcga tgttttgaac gactgagacc gcgttctcgc
     1561 agaggaacgt catgttggcg acggtgcctt cacgccggcc ggtgcgcccc atgcgctgca
     1621 agaaggacga gacggtgctt ggtgcgttcg cctgaaaggt gaggtcgagc ccgcccacgt
     1681 cgatcccgag ctccagcgtg gaggtcgcca cgatgcaggc attggttccg cgcgcgaact
     1741 tttcctctgc cgcccggcgc tcctccttgg acaccgagcc atggtgcacg aacacctcga
     1801 tcgactcgcc gcgcatctcg tcggccacgg cttcggtgag agcgcgcgac tgacagaaga
     1861 agagactctt cttccccagc gactcacggc tcgcttcacg ggcaaagtcg ctcacgtcct
     1921 cgcgaaggag gatcttgatc tgccgctggg ccttgggttt cggcgggtcg atgacgaccg
     1981 cttcgcggcg tgaggtgccg cggagccact ccgcgatgtg ctccgggttg ccgaccgttg
     2041 cgctgaggcc gatgcgctgc acgtcatgtt tcgacagcgc caccagacgc tcgatgacgc
     2101 acatgaggtg cgcgccgcga tcggtacccg caaatgcgtg cacctcgtcg atgaccacgc
     2161 tttggagctc gccgaagagg cgctgcaccg ggacgcgcgg agacatcagc atcacctcca
     2221 gcgactctgg cgtcgtcatg agcaacgccg tcggctcgcg gcagaagtgc tctttctgac
     2281 tctgtgccgc atctccgtgc cacacgaagc ggtcgagccc gaccatctgc gtgtagtcgc
     2341 cgagtcgcac ctcttgattg ttgagcagag ccttgatggg cgcgacgtag atcgctccga
     2401 cgccgctcgg cggattctct agaagctgag cgaggatcgg aaagatcgaa gcttccgtct
     2461 tgccgcccgc tgtcggcgcc agcaccactg cgttcttcct gtcgaggatc gcctggcccg
     2521 ccagctcctg caccgggcgg agcgaggaga aaccgagatg gttcgcgatg gcgtgctgga
     2581 gacgctcagg gaagcgggag aaaacgctca tgaccgtgtc cccgcgctca cgcatcgtaa
     2641 cggccatcgt cccgcgcgag a
//
LOCUS       DASTKS010000129         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_242354, whole genome shotgun sequence.
ACCESSION   DASTKS010000129 DASTKS010000000
VERSION     DASTKS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242354"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFQ35_02720"
     CDS             <1..291
                     /locus_tag="VFQ35_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9929567.1"
                     /translation="ERGENVDGLPGTEGAFLACSFWLVDAYVLSGRREEGERLFRRLL
                     GLANDVGLLSEQYDTQKKRLIGNFPQAFSHVALVNSARNLTAELRPAEQRSL"
     gene            complement(299..1195)
                     /locus_tag="VFQ35_02725"
     CDS             complement(299..1195)
                     /locus_tag="VFQ35_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9929568.1"
                     /translation="MAAKPEDLEWEPLRHFLAAARERSLSGAARRLGVEHSTVGRRIA
                     SLEQSLGAALVERGPAGLELTRLGTRVARLAKEMEHAAQRIAKLASAKRSTVRLVAPT
                     GFLALLSPGLARLRKAEPGVALEIVSAGRRVDLRNGAADLAIRVGPSEDEHLVTRKVG
                     EVAFALYAARSYLDRHGRPADLDDLSEHSVVGFQRGLSKMPAAEWLASRSSKATVVMR
                     SREAVDLLTAVQSGAGLGVLPCFLARGDASLERLTAKPVALRPVSLVYRREARLSPQV
                     RAVMRFVVEALRARARELNGER"
     gene            1298..2158
                     /locus_tag="VFQ35_02730"
     CDS             1298..2158
                     /locus_tag="VFQ35_02730"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET9929569.1"
                     /translation="MIVVLGASGRTGQVVARQVRAGSEAVRLVTRSAAIRADEPGVEV
                     VRARLSDGEELRRAFAGARASYVLLPEELGAQEIHAERRRWVETLVSALRRAAVEHVV
                     LLSSRAAALGETAESGFARELALLERRLFESVPAVTVLRASYFQDNVLQAVPTAARDG
                     VYLNCLSETASVDMTAACDVGEVAARALLRQPRVEKEVVDVVGPAYSPVEVASALGDL
                     VGRPVSVTFVPPDAREGLFQRWMSREAARALRETLDSVSASVALRGSRCERGATRLEQ
                     VLRAAREVTK"
     gene            2155..2625
                     /locus_tag="VFQ35_02735"
     CDS             2155..2625
                     /locus_tag="VFQ35_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929570.1"
                     /translation="MIAHVSLPALDCAFVANVLARMLGGGALRFPPGGPDAWNCWSRE
                     NDFQIVVTPRGRVIVPGEHGQEWSALPERERPEPLGYESHVAIAVPLEAREVVELARS
                     VGWRVGVFSRGGMFEVVEVWVENVYLVELLDPRQMADYRRTMTVDNWRRAFGLE"
     gene            2671..3153
                     /locus_tag="VFQ35_02740"
     CDS             2671..3153
                     /locus_tag="VFQ35_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929571.1"
                     /translation="MNAVTRAEAIRTRIDFERIRAYIVGFGLVGAVVAPFFWERGKDS
                     FPISSYPMFARPRPKTVVSFADAIDASGRAQRLSPEFVANDEVMQAAHIVRNAIEAGP
                     ERLAALCGRAAQRVAADPLLSSLVKVRLVQARFDSVGYFVRANEPEACTVFYECPVPR
                     "
     gene            3135..4058
                     /locus_tag="VFQ35_02745"
     CDS             3135..4058
                     /locus_tag="VFQ35_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9929572.1"
                     /translation="MSRSEMSVFGAIDRYWFSPAPAQRLALLRIAVGGFALGYLIWRF
                     DTLTSAAHFHPNEFAPVGVVKLLPRPLAPGSVSAVAIGAILAGIGFVLGYGYRLTGPL
                     FALSFLWVASYRNSWGMIFHVENLASLHLLLLGAAPAADALALGARRNVDVAASHGRY
                     GWPIRAMVVVTVVTYVLAGVAKLRFAGLDWADGHFLREQIAHDNLRKIELGSTHAPFG
                     AALVQYSLPFSVLAVLSLCLELGAPIALLGRGVARAWVSAAWGFHLGVAVMMAITFPY
                     QLTLIAFLPFFELERWRWVGWVTARFEAWRR"
     gene            4196..4801
                     /locus_tag="VFQ35_02750"
     CDS             4196..4801
                     /locus_tag="VFQ35_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9929573.1"
                     /translation="MKIYQIARCPFAHRARVVLNEKRLEYSIAYFESKHRPSELVDVS
                     PDARSPTIFDDEYQSWVWDSSVVAEYLEERYPAVPLLPSDPALRARVRLLMREVDSKL
                     GPIAGPLSEEFVHKPPEARDLSNADALLSRLHRALEAWERRAQTQSFFSSESLTLADV
                     WLYTQLFSVSGLVGWDRVLPEGLPHLRGWRDRLAARPSTVY"
BASE COUNT          740 a         1513 c         1712 g          921 t
ORIGIN      
        1 gagcgcgggg aaaacgtgga cggcttaccg gggacggaag gcgcctttct cgcgtgttcg
       61 ttctggctcg tggacgccta cgtgctcagc gggcgacgtg aggagggcga gcggttgttt
      121 cggcgtttgc tcgggctcgc gaacgacgtg ggcctactct ccgagcagta cgacacgcag
      181 aaaaagcgcc tgatcgggaa ctttccgcag gccttctccc acgttgcgct cgtgaatagc
      241 gcgcggaacc tgacggcgga gcttcgaccc gccgaacaac gctcgctgtg actttacgtc
      301 agcgctctcc gttgagctca cgggcgcgcg cgcgcaacgc ctcgacgacg aagcgcatca
      361 ccgcgcgcac ctgcggggac aagcgcgctt cacgtcgata gacgagtgac acgggccgga
      421 gtgccaccgg cttcgccgtc aaacgttcga gcgatgcgtc gccccttgcc agaaagcagg
      481 gcagcacacc gaggcccgcg ccgctctgta ccgccgtgag caggtcgacc gcttcgcggc
      541 tgcgcatcac caccgtcgcc ttcgacgagc gggaggcgag ccactctgcg gcgggcattt
      601 tcgagagacc acgctgaaaa ccgacgacgc tgtgttcgct gagatcatcc aagtcggcgg
      661 gcctgccgtg gcgatcgagg tacgagcgtg ctgcgtagag cgcgaacgct acctcgccca
      721 ctttccgcgt cacgaggtgt tcgtcttcgc tcgggcccac gcggatggcc agatcggcgg
      781 cgccgttacg caagtcgacg cgtcgcccgg cgctgacgat ctcgagcgcg acccccggct
      841 ccgccttgcg cagtcgcgcg agccccggcg agagcagcgc gagaaagccc gtcggtgcga
      901 ccaaacgcac ggtcgaccgc ttcgcgctcg cgagctttgc gatgcgctgc gccgcgtgct
      961 ccatctcctt tgcgaggcgc gcgacgcgag tcccgagccg cgtgagctcg aggccggccg
     1021 gacctcgctc gacgagcgcg gccccgagcg attgctcgag gctagcgatg cgccgaccaa
     1081 ccgtgctgtg ttctacgccg aggcggcgcg cggcccccga caagctgcgt tcgcgcgccg
     1141 cggcgaggaa gtgacgcagg ggctcccact cgaggtcttc gggttttgcg gccatgggta
     1201 tgagtgtagc gcgatgtgca tttttgcaca gcgctgtgcg cggctgcgga ctgccgccac
     1261 gtacgcctgc gccgcatgct gagcgcagga ggtcgcgatg attgtagtct tgggagcatc
     1321 gggaagaacg gggcaggtag tggcgcgcca agtgcgagcg gggagcgaag cggttcggct
     1381 cgtcacgcgg agcgcggcga ttcgagcgga cgagccgggc gtcgaggtag ttcgggcgag
     1441 gctttccgac ggcgaggagc tcagacgagc gtttgccggc gcgcgagctt cgtacgtgct
     1501 gttgcccgag gagctcggcg cgcaggagat tcatgccgag cggcgtcgtt gggtcgagac
     1561 actggtgagc gcgttgcgcc gcgcagccgt ggagcacgtg gttctgcttt cgtcgcgcgc
     1621 cgccgcgctc ggtgaaacag cggagagcgg gttcgcgcga gagctcgcgc tgctcgagcg
     1681 ccggttgttc gagagcgtgc ccgcggtgac cgtgctgcga gcctcgtatt ttcaggacaa
     1741 cgttctgcaa gcggtgccga cggcggcgcg tgacggcgtt tacctgaact gcctttcgga
     1801 gacggcatcg gtggacatga ccgcggcgtg tgacgtgggg gaggtcgccg cgcgcgcgtt
     1861 gctgcggcaa ccccgggtgg aaaaggaagt cgtcgacgtc gtcggcccgg catactcgcc
     1921 ggtggaggtt gcgagcgcgc tgggcgacct tgtgggcaga ccggtttcgg tcacgttcgt
     1981 cccgcccgac gcaagggagg gactatttca gcgctggatg tcgcgagaag ctgctcgcgc
     2041 gcttcgagaa acgttggatt cggtcagcgc gtccgtcgcg ctgcgaggaa gtcgctgcga
     2101 gcggggagcc acgcggctcg agcaagtgct tcgggccgcc cgcgaggtga ccaaatgatc
     2161 gcgcacgttt cgctaccggc gctcgactgc gctttcgtgg ccaacgtcct ggcgcgcatg
     2221 ctaggtggag gcgcgctgcg cttccctccg ggagggccgg acgcgtggaa ctgctggtcg
     2281 cgcgagaacg actttcaaat cgtcgtcacg ccgcgcggtc gcgtcatcgt tccgggtgag
     2341 cacgggcaag aatggtcagc gctcccggag cgggaacggc cggagccatt gggttacgag
     2401 agccacgtcg cgatcgccgt cccgctcgaa gcgcgggaag tcgtggagct ggcacgttcg
     2461 gtgggatggc gcgtcggtgt cttcagtcgc ggcggcatgt tcgaagtggt cgaagtgtgg
     2521 gtggaaaatg tctacctggt ggagctgctc gacccacgcc aaatggccga ctaccgacgc
     2581 acgatgacgg tcgacaattg gaggcgcgct ttcggcctcg aatgaacgtg tcgacgtcga
     2641 ggatttgttg tagcttccgc cccggcgacg atgaacgcgg ttacgagggc tgaggcgatt
     2701 cggacgcgca tcgatttcga gcggatccgc gcttatatcg tgggtttcgg cctcgtcggg
     2761 gccgtggtcg cgccgttctt ttgggaacgc ggcaaggatt cgttcccgat ctccagctac
     2821 ccgatgttcg ctcgaccgcg gccgaagacg gtggtgtcgt tcgcggacgc aatcgacgcg
     2881 agcggtcgcg cccagcgcct ctctccggag ttcgtcgcga acgacgaggt catgcaggcg
     2941 gctcacatcg tgcgcaacgc gatcgaggcg gggccggagc gcctggccgc gctctgtggc
     3001 cgggcggcgc agcgggtagc ggcggacccg ctgctgtcgt ccctcgtgaa agttcgtttg
     3061 gttcaagctc ggttcgactc ggtggggtac ttcgtgcgcg cgaacgagcc cgaagcttgc
     3121 accgtgttct acgagtgtcc cgttccgaga tgagcgtgtt cggggccatc gatcggtatt
     3181 ggttctcgcc cgcgccagcg cagcggctcg cattgctgcg gatcgcggtt ggggggtttg
     3241 ctctcgggta tctgatttgg cgtttcgaca ccttgacgag cgcggcgcac tttcacccga
     3301 acgagtttgc tcccgtcggc gtcgtgaagc tgcttccgcg accgctcgcg ccgggctcgg
     3361 tttctgccgt cgcgatcggg gcaatactag ccggaatcgg cttcgttctc gggtacggtt
     3421 atcggctcac cggtcccttg ttcgcgctct cgtttttgtg ggtggcaagc tacagaaatt
     3481 cgtggggcat gatcttccac gtcgaaaacc tggcttcgct gcacctgctc ttgctcggtg
     3541 ccgctcccgc cgcggacgcg ttggcgctcg gcgcacggcg caatgtcgac gtcgcggcga
     3601 gccacggccg ctacggatgg ccaatccgcg cgatggtcgt agtcacggtg gtgacgtacg
     3661 tgctcgctgg cgttgccaag ctcaggttcg ccgggctcga ttgggcggac gggcattttc
     3721 tgcgcgagca aatcgcgcac gataacttgc ggaagatcga gcttgggagc acccacgcgc
     3781 cgttcggcgc cgcgctcgtg cagtatagct taccgttcag tgtgctcgcg gtgctctcac
     3841 tctgcctaga gctcggcgcg ccaatcgctc tgctcgggcg aggcgtggca cgcgcgtggg
     3901 tttcggcggc ttgggggttt cacctcggtg tcgcggtgat gatggcgatc acgtttccgt
     3961 atcaactgac cctcatcgca ttcttaccct tcttcgagct cgagcgttgg cgctgggtgg
     4021 gctgggtaac cgcgcgattc gaagcttggc ggcgctgatc ggcgctcgta actcggacga
     4081 cgctccctat tccaagcgcc cgccgcgcaa gccaagaagc ccggtaaaag ccaacgagca
     4141 agagtggcag aagcgccgca tggctaaagc gcgttgggcg tgctgaggtt cgcccatgaa
     4201 gatttaccaa atcgcgaggt gcccgttcgc gcatcgggcg cgcgtggtgt tgaacgaaaa
     4261 gcggctcgag tattcgatcg cctacttcga gtccaagcac cgcccgagcg agctcgtcga
     4321 cgtgagcccg gacgcgcggt ccccgaccat tttcgacgac gagtaccaat cctgggtttg
     4381 ggattcgtcg gtcgtcgccg aatacctcga ggagcgttac ccagcggtgc cgctgctgcc
     4441 ttcggacccg gccctgcgcg ctcgcgtgcg gcttttgatg cgcgaggtgg actcgaagct
     4501 cggacccatc gccggccccc tcagcgagga gttcgttcac aagccgcccg aagcgcgcga
     4561 tctcagcaac gccgacgcgc tgctctcacg cttgcatcgc gctctcgaag cgtgggagag
     4621 gcgagcgcaa acgcagagct ttttctcgag cgagagtctc acgctcgccg atgtgtggct
     4681 ctacacgcag ctgttctcgg tttccggcct ggtcggttgg gaccgtgtgt tgcccgaagg
     4741 gctgccgcat ttgaggggct ggcgcgaccg gctcgcggcg cgtccgagca ccgtctactg
     4801 attcgatgat agaaatgcag caatttcgag tgtttgcgcg aatttttcag ggcctgtcga
     4861 tctccggagc gtctgttcgt cgcacc
//
LOCUS       DASTKS010000130         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1804997, whole genome shotgun sequence.
ACCESSION   DASTKS010000130 DASTKS010000000
VERSION     DASTKS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804997"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFQ35_02755"
     CDS             <1..507
                     /locus_tag="VFQ35_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929574.1"
                     /translation="VTCLAELPTSMTGSARGVTTRFDISSSEYPLASPTAMVVSSAKP
                     FCTHVSPDSGGVCLGGLWRTLQGKELLAELVLRVFQAFNHQDPNTSEPGYQPHARALR
                     ERLNGRPFNPKFVLPAVPDDVFHDDGPRVVRARVTIVGMPARASGSRVQLPSSVRPRI
                     RIVSGGGL"
     gene            504..1424
                     /locus_tag="VFQ35_02760"
     CDS             504..1424
                     /locus_tag="VFQ35_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HET9929575.1"
                     /translation="MTSRVVLVSARQEPPVRERGLARDGWRVFRSPNTGFEIALPAYQ
                     VSRVEAIAKREHTEWYALAVGELLRDDKGVHAVVRDFVPNDWAERGRAHVRMSATAEA
                     KVRELARELHPNSHQLGNLHTHPHFTTEPSSTDRQEFWSDPNSVSIIVDPFDSPTIAI
                     YRGREGEQLVEVTGGDEPRPPTPAPAATPTAPSAPVAPTCPIAPHLTPPCVLAVAPPV
                     VTERFDARALAGALVLSALFLGCFAVLGYSVERRLADVAAMNVDVASTNASLRERVRA
                     LEVQVRLSGATKKPEDGDEDTWDSEYEPAP"
     gene            1428..1724
                     /locus_tag="VFQ35_02765"
     CDS             1428..1724
                     /locus_tag="VFQ35_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929576.1"
                     /translation="MFRRLETALVAGAHALVVAMVLMVALQAVVLPSVPGGGGAVCLV
                     GVPLWALGRFSWSRRHAPVREERLKPRRRALPPAPMAGDEDVALPQIRPDRRTP"
     gene            1681..3291
                     /locus_tag="VFQ35_02770"
     CDS             1681..3291
                     /locus_tag="VFQ35_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929577.1"
                     /translation="MSLFRRFARIVARRDRRIAFGRERERVRAYVGASNPADDAPAGS
                     DLLDERGLPRDGEAERHFVVGARADDSERLVTLARNEVVASHSITFGATGAGKGVAWL
                     MLLDQELRRMILREELGLPPDTAILILEPKHDIAPAFLALLEDRLRSASPSVRERMLT
                     RLTTFNPLGRYVVPLPLLTPARGVAPELHATALSGLVGRLSGSPFGPKQRPILDVVLL
                     CWILARKVLPEGVELLGNWDRLRVLAQRSPSPVVRGFFEENARVPAGSLDGIRARLLR
                     LVFAPNLRAMFSSHTGLDFDEILTPGNIAVFDNGGDQGDEDLTAFFNGITVLEIGRAI
                     RRRANGAAPLIMLVDEFQRVLLGDGDVAEQTATLLETARSRGAAVHLLTQSPASVSAV
                     SPRLLRSIQTNAAVEILGATDDAAALAGILPVTGRRRRPENAPWEAAPSSPWLSREEE
                     QRALIEATQALPSRHFWVRPKRRLPKAVLTKTLEFHVPRSHGDELARRIEQGRWGRLP
                     EGDGGLRCAPRVTVISEAHAPPRRPRGL"
     gene            3589..4479
                     /locus_tag="VFQ35_02775"
     CDS             3589..4479
                     /locus_tag="VFQ35_02775"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HET9929578.1"
                     /translation="MNAHKVAKSRARRGVPQPTSLTRPGRKERRALLLTVWAGFLTTA
                     QIAEAAGYSSLRRAQRRLRTLLDRGHLRVELQSCALHLPSLHVPTRRAIETLIEDGEL
                     PAGYRPPRLPRAQKRPHALLIRTVFIAFAAAETRGALRLTDFRFDSHLTREEPFHGIG
                     LIPDAIATLALPDGSYATIAVEADAGTETTTTLRKKFAKWRAILDMWQPPRFTLLVVA
                     EREGRRRTLARLMTEARIGDVGLAVVAAEVRGFVASLAEPHGPAARSDRAERRTRVLE
                     PREVAGDRHVVGAAFRVIVR"
     gene            4781..5116
                     /locus_tag="VFQ35_02780"
     CDS             4781..5116
                     /locus_tag="VFQ35_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929579.1"
                     /translation="MTQTSPIPAEPWMKDASLTWYALVLLAMMELGGQRVPVQDIYEL
                     LRHHPKAAGTKHWQARIRATLRRNQAAVSSGGRGTWSLCCSPQDQREFLRALPPEAKP
                     TSRSRTVDR"
BASE COUNT          759 a         1753 c         1754 g          946 t
ORIGIN      
        1 gtgacgtgcc tggccgagct ccccaccagc atgacgggga gcgcaagggg cgtcacgacg
       61 cgtttcgaca tctcgagcag cgagtatcct ctcgcgtcgc cgactgcgat ggtggtcagc
      121 agcgcgaagc cgttttgcac gcacgtctcg ccggacagcg gaggtgtgtg cctgggtgga
      181 ctctggcgca cgcttcaggg caaagagctc ttggcggagc tcgtgcttcg cgtcttccag
      241 gcgttcaatc atcaagaccc caacacctcg gaacccggat accagcctca cgcgcgagcc
      301 ctgcgcgaac ggctgaacgg ccgtcccttc aacccgaagt tcgttcttcc agcggttccg
      361 gacgacgtgt tccacgacga cggtccacgg gtggtccggg cgcgggtcac gatcgtcggt
      421 atgcctgcgc gagcatccgg gtctcgcgtt cagctgccga gctcggttcg gccgcggatc
      481 cgcatcgtct cgggaggtgg actgtgacct cgcgggttgt cttggtctct gcgaggcaag
      541 agccgcccgt tcgcgagcgc ggcttggcgc gtgacggctg gcgcgtgttc aggtcaccga
      601 acacgggctt tgagatcgct ctcccagcct accaggtctc gcgggtcgaa gcgatcgcga
      661 agcgcgagca caccgagtgg tatgcgctcg cagtcgggga gctcctccgt gacgacaagg
      721 gcgttcacgc cgtcgtgcgc gacttcgttc cgaacgactg ggcagagcgc ggccgggccc
      781 atgtgcgcat gagcgcgacc gccgaggcga aggtccgtga gctcgcgcgc gagctccatc
      841 cgaactcgca ccagctcggg aacctgcaca cgcacccaca cttcacgacg gagccctcgt
      901 cgacggatcg ccaggagttc tggagcgacc cgaacagcgt ctccatcatc gtggacccgt
      961 tcgattcgcc gaccatcgcg atctatagag gccgtgaggg tgagcaactg gtcgaggtga
     1021 ccggtggcga cgagccgcgg ccgccgacgc ccgcgcccgc tgcgactccg acagcaccta
     1081 gcgcgccggt tgctccaact tgtccgatcg ccccacatct gacgccgccg tgcgtcctcg
     1141 ccgttgcgcc gccggtcgtg accgagcggt tcgacgcacg ggcccttgcc ggtgctctcg
     1201 tgttgtcggc gctgttcctc gggtgcttcg ctgtgctcgg ctacagcgtc gagcggaggc
     1261 tcgctgacgt tgcagccatg aacgtggatg ttgcatccac gaacgcgtcg cttcgcgaac
     1321 gggtgagggc gctcgaggtc caagtgcgct tgagcggcgc taccaagaag cccgaggacg
     1381 gggacgaaga cacctgggac tccgagtacg agccggcgcc gtgatcgatg ttcaggaggc
     1441 tcgaaactgc tctggtcgcg ggcgcgcatg cgctcgtcgt cgcgatggtt ctcatggttg
     1501 ctcttcaggc cgtcgtgttg ccgtccgtgc ctggaggtgg gggcgcggtg tgcctcgtcg
     1561 gcgtgccgct ttgggcgctc gggcgcttct cgtggagccg gcgccacgcc cccgtgcgtg
     1621 aagagcggtt gaagccgcgt cggcgggcgc tgccgccggc gcccatggcc ggagacgaag
     1681 atgtcgctct tccgcagatt cgcccggatc gtcgcacgcc gtgaccggcg catcgccttc
     1741 gggcgcgagc gggagcgcgt ccgcgcgtac gtcggagcct cgaaccctgc cgacgacgcg
     1801 ccggctgggt ctgacctgct cgatgaacga gggcttcctc gcgacggtga agctgagcgg
     1861 catttcgtcg tcggcgcgcg cgctgatgat tccgagcgtc tcgtaacgct cgcccgcaat
     1921 gaggtcgtcg catctcactc gatcacgttc ggcgcgaccg gtgcaggcaa aggcgtggcc
     1981 tggctcatgc tgctcgacca ggagctccgc cggatgatcc tgcgcgaaga gctcgggctt
     2041 ccgcccgaca cggccatcct gattctcgaa ccgaagcacg acatcgctcc ggcgtttctc
     2101 gccctgctcg aggaccgtct tcgctctgcg tcgccctcgg tacgcgagcg catgctcacg
     2161 aggctcacga ccttcaatcc gctcggccgc tatgtcgtgc cgcttccgct cctcacgccc
     2221 gcgcgcggcg tggcgccgga gctccacgcg acggcgctct ccggcctcgt cggtcgtctg
     2281 tcgggctcac cgttcgggcc caagcagcgg ccgatcctcg atgtggttct gttgtgctgg
     2341 atcctcgcgc gaaaggtcct tcccgaggga gttgagcttc tcggcaactg ggaccggctg
     2401 cgtgtgcttg cgcagcgctc gccatcgccc gtcgtacgcg gcttcttcga ggagaacgcc
     2461 cgcgttcccg ccggcagcct tgatggaatc cgcgcgcgcc tccttcgtct cgtctttgcc
     2521 ccaaacctcc gagcgatgtt ttcgtcgcac acgggcctcg acttcgacga gatcctcacc
     2581 cccggcaaca tcgcggtgtt cgacaacggg ggcgatcagg gcgacgagga tctcaccgcc
     2641 ttctttaacg gcatcaccgt gctcgagatc ggccgtgcga tccgccgccg agcgaatggc
     2701 gcggcgcccc tgattatgtt ggtcgacgag tttcagcggg tgcttctggg ggatggcgac
     2761 gtagctgagc agaccgctac gctgctcgag accgcccgtt cgcggggcgc cgcggtccat
     2821 ctgctgactc agagtcccgc ttcggtttca gccgtctccc cacgcctgct ccgctcgatc
     2881 cagacgaacg cagccgtcga gatcttgggt gcgaccgacg acgcggccgc gctcgccgga
     2941 atccttcctg tgaccggtcg tcgtcgccgt ccggagaacg cgccgtggga ggcggcgccg
     3001 tcgtcgccct ggctctcgcg cgaggaggag cagcgcgcac tcatcgaggc gacccaagcg
     3061 cttccttcgc gtcacttctg ggttcggccc aagcgtcggc tgcccaaggc cgtgctcacg
     3121 aagacgctcg agttccacgt tccacgaagc catggtgacg agctcgcccg gcggatcgag
     3181 caaggacgtt ggggtcgcct gccagagggc gacggcggcc tacgctgcgc gccgcgggtg
     3241 accgtgatca gcgaggctca cgcgccaccg cggcggccga gggggttgta ggacgatgtc
     3301 cctcgcacgt gagttcgtgc tcgcccccgc ctacgcagcc tatggcgtgt gggttgtcct
     3361 tcgtgctgcg cggcgtggca gccgcgaact tcgcgcgctc agggggctct tcgatacgca
     3421 cgtcgtctgc cgcacgtgtg gcttcgagaa cgcggtgcac gaccgctgga gctgtgccga
     3481 ctgcggtgct gagtacgccg gcgccgtgca cacctgtggc ctttgcggtg ccggggcgtc
     3541 gtggtttccg tgcgcgcggt gccgcgcgtc catcccgctg ggactcccgt gaacgctcac
     3601 aaggtcgcga agtcgcgagc ccggcgcggc gtaccccagc cgaccagttt gacgcgccct
     3661 ggtcggaagg agcgccgcgc ccttctgctc acggtctggg ccgggttcct cacgacggca
     3721 cagatcgccg aggcggccgg gtactcgagc ctccgaaggg cgcagcgccg gctccgcacg
     3781 ttgctcgatc gcggtcacct tcgcgtcgag ctacagagct gtgcgctcca ccttccttcg
     3841 cttcacgtgc cgacgcgtcg cgcgatcgag acgttgatcg aagatggcga gcttcctgcc
     3901 ggctaccggc ctccacgcct gccgagggcg cagaagcggc cccacgcgct cctcatccgg
     3961 acggtcttca tcgccttcgc ggcggctgag acgcgaggtg cccttcggtt gaccgatttt
     4021 cgatttgatt cgcacctgac gcgcgaggaa ccgttccacg ggatcggcct gatcccagat
     4081 gcgattgcca cgttggcgct accggatggg tcgtacgcca cgatcgccgt cgaagcggat
     4141 gccgggaccg aaaccacgac gacgttgcgc aaaaaattcg ccaagtggcg agccattctc
     4201 gacatgtggc agccaccacg cttcacgttg ctcgtcgttg ccgaacgaga aggccgacgg
     4261 cggacgctcg cgcggctcat gacggaggcc cggatcggcg acgttggtct tgccgtggtc
     4321 gcggcggagg tgcggggatt cgtggcgagc cttgccgagc cgcacggccc cgccgcgcga
     4381 agtgaccgtg ccgaacggcg tacgcgcgtt ctcgaacccc gcgaagttgc aggggatcgt
     4441 cacgtcgtcg gcgcggcgtt tcgggtcatc gttcgatgat ccggttagtt tgcgggaggg
     4501 tgggaatgaa cgcacgaggc gcgtcgtcat ggctgagcgg taggtcctgc tggtgcagca
     4561 cctctcttgt tcgtgaactt cggaagaacc gctcgcgcag gcgtcgtcat ggctctcggc
     4621 atcgtcccct tgccgaccca cgcggagatg gggttcgttt caaggcgtgc aacgacgcgc
     4681 cgaaaggagg attgggcact cttcgtgatc tgttggtcga tcgccttggt cgttcggctt
     4741 cgtcatgcgc tggtccgtgc cgaccccggc ttgaaccccc atgactcaaa catcgccaat
     4801 accggcagaa ccgtggatga aggacgcctc cctcacgtgg tacgcgctcg tcctgctcgc
     4861 catgatggag ctcggtggtc aacgggtgcc tgtgcaagac atctacgaac tcttgcgcca
     4921 ccacccgaag gctgcgggca cgaagcactg gcaagctcgc attcgcgcca cgctccgaag
     4981 gaatcaggcg gccgtttcct cgggtggccg cggaacttgg tcgctgtgct gcagccccca
     5041 ggatcaacgc gagttcttgc gcgcgttgcc tccggaggcg aagcctacgt ctcggtcgag
     5101 gacggtagac cgctaacgga tcatgtcagg gtaaccattc cacggaggtg tgcggcgcgg
     5161 ccggggtcgg tttgctcgag ccgtcgcatg agtcgcgaac gcctgaggca ga
//
LOCUS       DASTKS010000131         6455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1135680, whole genome shotgun sequence.
ACCESSION   DASTKS010000131 DASTKS010000000
VERSION     DASTKS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135680"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..521)
                     /locus_tag="VFQ35_02785"
     CDS             complement(3..521)
                     /locus_tag="VFQ35_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9929580.1"
                     /translation="MAITRKTTYALRALAGMAKAPNELHSVTSMVEVEGIPKAYLSAI
                     LNTLVRYGLILSRRGPSGGYVLAKDPTEISLARVVEVLEGPLFPVGCLGPDSEPCREC
                     GSGCAARWALGAAARAAWQALESICVADLTHCPSAKIDRHVYLPVFAEKHAPARSLLV
                     PLPSSPHRDPKP"
     gene            complement(625..948)
                     /locus_tag="VFQ35_02790"
     CDS             complement(625..948)
                     /locus_tag="VFQ35_02790"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HET9929581.1"
                     /translation="MHIGLVGGVERGERRYASLAEASGHSFEVHSGDMAGRGGEVLEA
                     LVERSDIVIVITDVNSHAAVLAARHHARAMGRRCLLVRRFGFSRFRSLLLELSAPAPA
                     VAKSA"
     gene            complement(961..1359)
                     /locus_tag="VFQ35_02795"
     CDS             complement(961..1359)
                     /locus_tag="VFQ35_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929582.1"
                     /translation="MQIATLSAVPRRRAPRLLARPDERRLCVIVDVHAEAARERLVAE
                     LERLPLPASVEMLRADEAVGLSPRVRIAADAEIEGELELHAETHGSGRDAALVFELVQ
                     ALAQGSFAVHVTFSETRSPRQLGFSSTNRR"
     gene            2555..3031
                     /locus_tag="VFQ35_02800"
     CDS             2555..3031
                     /locus_tag="VFQ35_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929583.1"
                     /translation="MTLDSELASAKALDSKEALVSGEEFALGAGLDSEEELASGVGLD
                     SGEELASGVGLDSGEDLASGVGLDSGEELASGVGLDSGKVDSSDELDSDEIDSADALA
                     LARALAFGMSPGRTEGTARSPRFFPFLPFLRIIPCASQYPCDRGAKAKAPARSNQA"
     gene            3510..4214
                     /locus_tag="VFQ35_02805"
     CDS             3510..4214
                     /locus_tag="VFQ35_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HET9929584.1"
                     /translation="MNHALRIFRNLRLDKIWLACCLLAAPSARGDSPRVQAWEKFDED
                     DGIAVYRRDVAGTPVVALRGEGIVDAPLLRVASVLVDTKRATEWIDSLAEARTVRRVS
                     ETEYVEWDHIATPFVLKDRDFVFDAKLEFKPAQKQVVLNYHSVNDKDAPKTDYIRGEF
                     IYGTFALTSVDGGKRTRVLAEVLCDPKGSVAKWIVNLFQKSWPHNTVVALREQVKKAD
                     IKDDPRLKAELARLGY"
     gene            4331..5953
                     /locus_tag="VFQ35_02810"
     CDS             4331..5953
                     /locus_tag="VFQ35_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9929585.1"
                     /translation="MIRLDSVTKAHGRQVLFVDASCALFRGEKVGLVGPNGAGKSTIF
                     RLIVKEETPDAGQVAIDRGTTIGYFSQDVGDMKGMSVVAATLQGAGPVAEVAERLKVL
                     EHALGDPAQADDLERLVTEFGEAQARFEELGGYALDAKAREILAGLGFSSEMIDGDVG
                     ALSGGWKMRVALARILLMKPDVMLLDEPTNHLDIESILWLEKFLQNFDGALMMTSHDR
                     EFMNRIVSKIVEIDGGELTSFSGDYEFYVRQRAQLDAQAESEFLRQKAMLAKEEAFIA
                     RFKARASHAAQVQSRVKKLEKIERVEPPKQRQRLRFDFPEAPRSGEDVVKFEGVSKGY
                     GQRVIYDGFDWLVRRKERWCVMGVNGAGKSTLLKLAARAITPDGGRVTLGASVELGYF
                     AQHSMELLNAESTVWETLVARFPQASIGSLRTLAGVFGFSGDAVEKGCRFLSGGEKAR
                     LVLALMLYNPPNFLVLDEPTNHLDMDTKEMLVEALANYEGTMLFVSHDRHFLRQLSNR
                     VLEVNPEGVRLYGGGYGEYVEETGQEAPGVRT"
     gene            complement(5968..>6455)
                     /locus_tag="VFQ35_02815"
     CDS             complement(5968..>6455)
                     /locus_tag="VFQ35_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929586.1"
                     /translation="AVAAASPQLSATIAAAQPDSALAEPSSVSASAAEEPRPAASAVD
                     AAAQVEGPSLPTAPPGKRAVVIEVVPWDAKVLLAGVAQPGLPFVVMIPEGRRVAFEVA
                     RRGYIPRRVVVDGSEPRLSVGLVRPRGARGEVPPPPPRPSEGEAEEGPKDSRLRVRSG
                     L"
BASE COUNT         1241 a         1999 c         2151 g         1064 t
ORIGIN      
        1 tttcatggtt tcggatctcg atgaggactg gacggaagtg ggacgagcag agagcgcgcc
       61 ggcgcgtgct tctccgcaaa cactggcaag tagacgtgcc gatctatctt cgctgatggg
      121 cagtgtgtga gatcggcgac gcagatggat tcgagcgctt gccaggcggc gcgcgcagcc
      181 gcaccgagcg cccagcgagc agcacaaccc gaaccgcact cgcgacaagg ttcgctatcg
      241 ggaccgaggc agcccaccgg aaacagtggt ccctcgagca cttcgacgac gcgagccaga
      301 ctgatctcgg tcgggtcttt agccagcacg taaccgccgc tcgggccgcg ccggctgagg
      361 attaagccgt aacgaacgag agtattcaga atagccgata gataggcttt tggaattcct
      421 tccacctcga ccatcgaagt caccgaatga agctcgttcg gcgccttggc catacctgcg
      481 agcgcgcgca gtgcataggt ggtcttcctc gtaattgcca aaagcctagt aaaacgatgg
      541 actaaattac tttggcgaca atgtcaacta cgtctgcgct gccccctctc attccgcagc
      601 ccgaagtgca gacccccgcg ggattcacgc cgacttggcg actgcaggag cgggggcaga
      661 gagctccagc aacaaggaac gaaagcgtga gaagccgaag cggcgcacca acaggcatcg
      721 gcgtcccatc gcgcgcgcgt ggtgtcgcgc cgcgagaacc gctgcgtggc tgttcacgtc
      781 cgtgatgaca atgacgatgt ccgagcgctc gacgagcgct tccagcacct cgccgccgcg
      841 gccagccata tcgccactgt ggacttcgaa cgaatgtcca cttgcctctg cgagcgatgc
      901 gtaacggcgt tcgccgcgct ccactccacc gacgagaccg atgtgcatgg catctcctgt
      961 ttagcgacga ttcgtgctcg aaaagcccag ttggcgcggt gagcgcgttt cggagaaggt
     1021 gacgtgaacg gcgaatgagc cttgggcgag cgcttgaacg agctcgaata cgagcgcagc
     1081 gtcccggcca gaaccgtgag tctcagcgtg aagctcgagc tcaccttcta tctcggcgtc
     1141 ggctgcgatc cgcacgcgcg gggaaagccc taccgcttcg tcggcgcgca acatctcgac
     1201 cgaagcgggc agaggcaatc gctcgagctc agccacgagc cgctcgcgtg ccgcttcggc
     1261 atgcacgtcg acgatgacgc acagccggcg ctcgtccggc cgggccaaga gacgaggtgc
     1321 gcggcgtcgc ggcaccgcag acaacgtggc tatttgcatg gaggcctcaa gttcgccacg
     1381 tgaaacgcgg ccgaattcag acatcttacg aacgaatgtt tgtctaatat aacagacacg
     1441 aagctcagcg caagccccac cctggctgca gcagagagaa aggctccgca cggcccgtcc
     1501 cgcaggggcg ctaaaggtcc gtgagggcgc taaaggtggc gagcgccacg accgacagtt
     1561 cagtcgctgc ggaaccagtc gagaattgat ccaatcgacg agtcgcgcga ggcgagcgac
     1621 ttcgagccgc tacggcgtga tggggaagcc agcttgagcg ggcgcttgcg gcttcgaagc
     1681 aggcgccgtc gcggcgctag aacctggcgc ggtcgacgat gccggtcgcg aactcgccgc
     1741 agcacgaagg gtgcgagttc gggaagccgc agatggcttg ggcgcgactt ttcgcttcaa
     1801 gggctttgcg cgaggtgcgg tacgcgccgg agcgctgcgg gtgttccccg aaggcgcaac
     1861 ggaaggtgcg aggggagcgg ctcgagtcgc gttcgtcgcg ctggacgccg ccgcggacgg
     1921 ggcagcaact gaaggcaaag cgctcggggc cgactgcgca ctggctgcgg agctcagagt
     1981 cgaactggtt tccgaattcg aaagctctgc ggttcctggc gacgacgccg ccattggatt
     2041 tacgtcgacg gacgctgccg ttgggggagg agcaatgatt gcaggtgctt cgactcgagc
     2101 tgggatgtcc ggcatcagct cgtcgggcgt cgcagcccga accgtcagcg cgttcacgtc
     2161 gatcgcatgc gccgcgactc cggctggcgc ctcctcgagg ggggacgaag cgaagaacgc
     2221 ttgcttcgcg aagatcgcga cgctcgccgc cccgagtacg accatggtcc gcgcgaccca
     2281 gcggcgaaac cgcgcccgcc gttcgagttg cgaagcggtg atcaggggcg gctcatcgaa
     2341 ttcttcgggc tccgactggt gctcgatatt ctcgatgctg atccgctgcc cgatagcgaa
     2401 aaagtcgtcg tactcgagcc attcgctcgg cggtggcggt gtgatggcgt caaccgcgaa
     2461 ttcgagcttc agcccatcac catcgccatc gccatcgcca tcgcggtcgc gcgctggtgc
     2521 ggggctcgac tcagaagagg agctcgaagc ggaagtgacg ctcgattcgg agctggcatc
     2581 agcaaaggcg ctcgattcga aagaggcgct cgtgtcagga gaagaattcg cgttgggtgc
     2641 agggctcgac tccgaagagg aactcgcctc gggtgtgggg ctcgactccg gagaggaact
     2701 cgcctcgggt gtggggctcg attccggaga ggacctcgcc tcgggtgtgg ggctcgattc
     2761 cggagaggaa ctcgcctcgg gtgtggggct cgactccgga aaggtcgatt cgtcggacga
     2821 gctggattcg gacgagatcg attcagcgga cgcactcgcg ctggcgcgcg cactcgcgtt
     2881 cgggatgagc ccggggcgaa cagaggggac agcccgtagt cctcgattct ttcccttcct
     2941 gcccttctta cgaatcatcc catgtgcctc gcaataccca tgcgatcgcg gcgcgaaagc
     3001 caaagctccc gcccgtagta accaagcctg aacgaggcaa aatcacgcga cacttggggc
     3061 agagtcaccg ctcgactcgt caatcaggca gcgctcggcc atttgaccgc gccagaaagc
     3121 cgcaacgcag cggcggtgaa ggttgccatc gctcggtttc ctagggtgag cgcaaagcgc
     3181 tctccagtcc agctccagcc cggagtccga acgagcgctc ggtacgctgc gagctggacc
     3241 gccgcgaggg acgcgagtca gctcgcgctg caagccagac aacggctcgc cttttcacgg
     3301 catggcgacg cagctcgact cgcccggacc cgccggggag ccgcaaactg aagcccaggg
     3361 ccacgagcga gttctcaccg cagaggattt tttgactgtc gacagctcag ccgtttccac
     3421 catgtatccg cacgatcgga ttgccatcgg ccgggagggg atcgcgttgc gggagctcgc
     3481 gcggcttcgt gtatcgtccc aaggcacccg tgaaccacgc acttcgaatc ttccgaaacc
     3541 ttcggctgga caagatctgg ctcgcctgct gcctgcttgc ggcgccgagc gctcgaggtg
     3601 actcgccgcg cgtgcaagct tgggagaagt tcgacgaaga tgacggcatc gccgtgtatc
     3661 gccgtgacgt cgcgggcacg ccggtggtgg ccttgcgcgg agaaggaata gtcgacgctc
     3721 cgcttctacg ggttgcttcc gttttggtgg acacgaagcg cgcgaccgaa tggatcgaca
     3781 gcctggccga agctcgaacc gtgcggcgag tgagcgaaac cgagtacgtc gagtgggacc
     3841 acatcgcgac gccgttcgtg ttgaaggatc gggacttcgt gttcgacgcg aagctcgagt
     3901 tcaagccggc gcaaaagcag gtagttttga actaccactc ggtgaacgac aaggacgccc
     3961 ccaagaccga ctacatccgc ggcgagttca tctatggcac cttcgccctc acttccgtcg
     4021 acgggggcaa gcgcacgcga gtcctagcgg aggtgctgtg cgatccgaaa ggctccgtcg
     4081 cgaagtggat cgtgaacctg tttcaaaaga gctggccgca caacacggtg gtcgcgcttc
     4141 gagagcaggt gaagaaggcc gacatcaaag acgacccgcg gctcaaagcg gagctcgcgc
     4201 ggctcggcta ctgatcacgt cgacgccggc gccgcccgcg gcggccgcat gccccggaaa
     4261 cgccgcggaa tttgccgaac gagcggctcg gacgtgcgac tcgaagcgcg ctctgtgcta
     4321 acgggggcgc gtgattcggc tcgattcggt taccaaagca cacggccgcc aagttctgtt
     4381 cgtcgacgct tcgtgcgccc tgttccgggg tgagaaggtt ggactcgtgg ggccgaacgg
     4441 cgccggaaag tcgaccatct tccgcctcat cgtgaaagaa gagacgccgg acgcagggca
     4501 agtggcgatc gatcgcggca cgacgattgg ctacttcagc caagacgtcg gcgacatgaa
     4561 gggcatgagc gtcgtcgccg ccactctaca aggagccggg cccgtcgcgg aggtggctga
     4621 gcgcttgaag gtgctcgaac acgcgctcgg cgacccggcg caagcagacg acctggagcg
     4681 gctcgtcacc gagttcggcg aagcgcaggc gcgcttcgaa gagctcggcg gctacgcgct
     4741 cgacgcgaag gcgcgagaga tcctggctgg gctcggcttc agtagcgaaa tgatcgatgg
     4801 tgacgtgggc gccctctctg gcggttggaa gatgcgcgtc gcgctcgctc gcatcctctt
     4861 gatgaagccg gacgtgatgc tgctggacga gccgacgaac cacctcgaca tcgagtcgat
     4921 cctatggctc gaaaagttcc ttcagaattt cgacggcgcg ttgatgatga cctcgcacga
     4981 tcgcgagttc atgaaccgca tcgtgagcaa gatcgtcgag atcgacgggg gtgaactcac
     5041 gagcttcagc ggcgactacg aattttacgt acgccagcgc gctcagctcg acgcacaagc
     5101 cgagagcgag tttttgaggc aaaaggccat gctcgcgaag gaggaggcat tcatcgcgcg
     5161 cttcaaagcg cgggcgagtc acgcggcgca ggttcaatca cgcgtgaaga agctcgagaa
     5221 gatagagcga gtggaaccgc ccaagcagcg gcaaaggctg cgcttcgatt tcccggaggc
     5281 gccccgctcg ggcgaggacg tcgtgaaatt cgagggcgtc agcaaaggct acgggcagcg
     5341 agtcatttac gacggcttcg attggttggt tcggcgcaag gagcgctggt gcgtcatggg
     5401 agtgaacggc gccggaaagt cgacgttgct caagctggca gcgcgggcca tcacgccgga
     5461 cggcggacga gtgacgctcg gcgcgagcgt cgagctcggc tatttcgcgc agcattcgat
     5521 ggagctcctg aacgccgaga gcaccgtttg ggagaccctg gtcgctcgct tcccgcaagc
     5581 ttccatcggc tcgctccgga ctctcgctgg agtcttcggc ttcagcggtg acgcggtgga
     5641 gaagggctgc cgcttcctat ccggcggaga gaaggcgcgg ctcgtcctcg cgctgatgct
     5701 ctacaacccg ccgaactttt tggtgctcga cgagccgacg aaccacctcg acatggacac
     5761 caaggagatg ctcgtcgagg cgctcgcaaa ctacgagggc accatgttgt tcgtctcgca
     5821 cgaccgtcac ttcttgcggc agctatcgaa ccgcgtgctc gaagtgaacc ccgagggcgt
     5881 cagactctac ggtggcggct acggggagta cgtggaagag acgggccaag aggcgcccgg
     5941 cgtccgcacc tgagcgtcgt cgcgccctca gagcccgctg cgcacgcgca accgcgaatc
     6001 cttcgggccc tcctcagcct ctccttcact cgggcggggc ggaggagggg ggacctcgcc
     6061 gcgcgcacct cggggacgca cgagcccgac cgatagcctg ggctcacttc cgtccacgac
     6121 gacgcgacgc gggatgtagc ctctgcgcgc aacctcgaaa gcgacgcgcc tgccctcggg
     6181 gatcatgacg acgaacggga gacctggctg cgcgacgccg gccaagagca ccttggcgtc
     6241 ccacggaacg acctcgatga cgactgccct cttccccggc ggcgcagtcg ggagtgacgg
     6301 accctcaacc tgcgcggcag cgtcgacggc gctcgcagcg gggcgaggct cctcggcggc
     6361 gctcgcggac accgacgacg gctctgccaa cgcgctgtcc ggctgcgctg cagcgatcgt
     6421 cgcggagagc tgtggagagg cggcggctac ggcag
//
LOCUS       DASTKS010000132         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1359262, whole genome shotgun sequence.
ACCESSION   DASTKS010000132 DASTKS010000000
VERSION     DASTKS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359262"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VFQ35_02820"
     CDS             <1..1852
                     /locus_tag="VFQ35_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9929587.1"
                     /translation="ISNLRQLQPDSRFLLVTGYADAAQRGSRLPGVSGVLVKPWHDRE
                     LVDLVSSAVEQAKAGRTTRETLRPAGASLVLMLEDSDVDALVLSRSVETASDGDFQVV
                     RFTRLADACASLKTRDYAVVLSDLSLPDAEGLAALSELSAVSPSTPVLVVTSADDEAQ
                     ALRAVQAGAQDYLLKGKFDGEGVLRAIRYAIERKATEKRLSELAHYDQLTHLANRTLL
                     NDRLAQALARSSRSGAGVALLLVDLDHFKAANDSYGHEFGDLLLVEVAERLTASTRRE
                     DTVARIGGDEFAVLLEELEDPEEARRVAQRILNAFATPLIVEGKVLPVTASIGVALYP
                     EHESDEAGLLRAADRALYRAKDLGRNCYAVTGEAGFDRGTRRAEIERALLSHVSDERF
                     GLALQPVVNLDDLNVVGHELLLRWTADDGEPVTAREFIALLEQTGDLLRVGRWVFRRA
                     CALAERDAPMCPLSVNVSLREFCDRDFLENVKGILKTHDIAHGNLALELSEGTLTKNL
                     AGARTMLPQLVALGVSITIDDYGSGRSSLVELGELPVSTIKLAGSLLHGASRPSGKAT
                     LSAVIAAARALGWTVIAKSVETADDVAMLRALGCTLAQGWMLGAPSLITR"
     gene            1912..>2673
                     /locus_tag="VFQ35_02825"
     CDS             1912..>2673
                     /locus_tag="VFQ35_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9929588.1"
                     /translation="MAATGRIRKRLALAIVLTALIPVLVSIWLAEKTMRNTVARLYLP
                     DTESHLVRSLEVYKELSRSVKSLMRQEAAAVAERESLRRAVRDGDRAAIERELTRAFA
                     EHPTLVTLSARDTDGRELGRVERARPLDPARENGLEVVRQLADGPEDDSPSLLAVFAA
                     NRARFDEHQEMNQFLETYRTLERRREADEQAYVLGFALLLAITIVFAIGVGVLLARGV
                     SERIAQLAQATHQVALGDLNIRVPEQGGDELSDLAA"
BASE COUNT          422 a          893 c          913 g          445 t
ORIGIN      
        1 gatttccaac ttgcggcagc ttcaacccga ttcgcggttc ctcctggtaa cggggtatgc
       61 ggatgcggca cagcgtggca gccgcttgcc cggcgtctcg ggggttctgg tcaagccgtg
      121 gcacgatcgc gagctcgtcg atctcgtgtc gagcgccgtg gaacaagcca aggcggggcg
      181 tacgacgcgc gagacgttgc ggcccgcggg ggcgtctctc gttttgatgt tggaggacag
      241 cgacgtcgac gcgctcgttc tatcgcgcag cgtagaaacc gcgtccgatg gtgactttca
      301 ggtcgtgcgc ttcacgcgcc tcgccgatgc gtgcgcgagc ttgaagacgc gcgactacgc
      361 ggtggtgctc tcggatttga gcttgcccga cgcggagggg ctggcggcgc tgagcgagct
      421 ctctgccgta tcgccgagca cgccagtgct cgtcgtgacg tcggccgacg acgaggcaca
      481 agcgctgcgc gcggtgcaag cgggcgccca ggattacctt ttgaagggca agttcgatgg
      541 ggaaggggtg cttcgcgcga tccgctacgc catcgagcgc aaggccaccg aaaagcgcct
      601 gagcgagctc gcccactacg accagctgac gcacctcgcg aaccgcaccc tgctcaacga
      661 ccgcctggcg caagcgctcg cgcgctcgtc gcgctccggc gcgggggtcg cgctcctgct
      721 cgtcgacttg gaccatttca aggccgcgaa cgacagctac gggcacgagt tcggcgatct
      781 gttgctcgtc gaggtcgccg agcgactcac cgcgagcact cgccgcgaag acaccgtcgc
      841 gcgcatcggc ggcgacgaat tcgccgttct gctggaagag ctcgaggatc ccgaggaggc
      901 gcgacgcgtc gcgcaacgca tcttgaatgc cttcgcgact cctctcatcg tggaaggcaa
      961 ggtgctcccc gtcaccgcga gcatcggcgt cgcgctgtat cccgagcacg aaagcgacga
     1021 ggcgggcctg ctgcgcgccg cggatagagc tttgtaccgc gcgaaggatc tcggtcgaaa
     1081 ttgctacgcc gtaacgggcg aggccggttt cgatcgcggc acccggcgcg ccgaaatcga
     1141 gcgcgcgctt ttgtcgcacg tgagtgacga gcgcttcggg ctcgccctgc agccggtcgt
     1201 gaacctcgac gacctcaacg tcgtcggtca cgagctcctg ctgcgctgga ccgcggacga
     1261 cggcgagccc gtgaccgcgc gcgaattcat tgccctgttg gagcaaaccg gagacttgct
     1321 gcgcgtcggt cgctgggtgt ttcgccgcgc ctgcgcgctc gccgagcgcg acgcgccgat
     1381 gtgtccgctc tccgtgaacg tatcgcttcg agagttttgc gaccgcgact ttctcgagaa
     1441 cgtcaaaggg atcttgaaga cgcacgacat cgcgcacggg aacctggccc tcgagctctc
     1501 ggaaggaacg ctgaccaaga acctcgcggg ggcgcgcacg atgctgcccc agctcgtcgc
     1561 gctcggggtg tcgatcacca tcgacgacta cggcagtggc cgctcgtcgt tggtcgagct
     1621 cggggagctc cccgtctcga ccatcaagct ggcgggctcc ctacttcatg gcgcgtcgcg
     1681 cccgagcggt aaggcgaccc tctcggccgt gatcgcggcg gcgagggcgc tcggctggac
     1741 cgtcatcgcg aaatcggtcg agacggcgga cgacgtcgcc atgctccgtg cgctcgggtg
     1801 cacgctggcg cagggctgga tgctcggcgc gccgtcgctc atcacgcgct gatctcggca
     1861 gaaaaggcgc cggcttgctg cttcgtcgcc tcatcgggca agatgcgctc gatggccgcc
     1921 acgggccgca tccgaaaaag gctcgcgtta gccatcgtcc tcacggcgct gatcccagtc
     1981 ttggtttcga tctggctcgc cgaaaagacg atgcgaaata ccgtcgcgcg gctgtacctg
     2041 ccggacaccg agtcgcattt ggtgcgctcg ctcgaggtct acaaggagct atcgcgcagc
     2101 gtaaagtcgt tgatgcgaca agaggcggcc gcggtcgccg agcgcgagtc gctgcgtcga
     2161 gcggtgcgtg acggtgaccg cgccgcgatc gagcgcgagc tcacgcgcgc cttcgccgag
     2221 cacccgacgc tcgtgacgct ctcggcacgc gacacggacg gccgcgagct cgggcgcgtc
     2281 gaacgagcgc ggccgctcga tccggcgcgg gagaacggcc tcgaggtcgt gcggcagctc
     2341 gcggatggcc ccgaagacga ctccccctcg ctcttggccg tgttcgcggc gaaccgcgcg
     2401 cgcttcgacg agcaccaaga aatgaatcag ttcctcgaga cgtaccgcac gctcgagcgc
     2461 cgccgcgagg cggacgaaca agcgtacgtc ctcggcttcg cgctgctgct cgcgatcacg
     2521 atcgtcttcg cgatcggtgt cggcgtgctc ctcgcgcgcg gtgtgtccga gcgcatcgca
     2581 cagctcgcgc aggcgacgca ccaggtggcg ctcggcgatc tcaacatccg cgtgcccgaa
     2641 caggggggcg atgagctctc ggacttggcg gcc
//
LOCUS       DASTKS010000133         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_689964, whole genome shotgun sequence.
ACCESSION   DASTKS010000133 DASTKS010000000
VERSION     DASTKS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689964"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ35_02830"
     CDS             <1..432
                     /locus_tag="VFQ35_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9929589.1"
                     /translation="PLEIEALSRVFAARFCAEHGRARLNITAECLRALERYSWPGNLR
                     ELRNAIERAVVLCQGDALLPEHLPPKVRAGKPTDIASEPPGDRRALLQKEFDDLERTR
                     IQAALDKAGGNQTTAAKLLGISRRTLVYRLTALQLPRPRKG"
     gene            556..1293
                     /locus_tag="VFQ35_02835"
     CDS             556..1293
                     /locus_tag="VFQ35_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929590.1"
                     /translation="MTMEVALVSMVTRAEGTQGNPGVKHDTVHEDLELLRRVRSGEPS
                     AREAFLVRVTDVVRVRVGRVLARASRNRAQADLRRRDLLDLMQDVFVVLLDRDAKVLA
                     AWEPERGLSLDGFVGLVAERETLAFLRSGRRSAWAEHPSEDIVDEGVEDVSPELQASA
                     KEELSLLLDYLSDRLSPQGALLFQALYVDHASLEDVCERFSMSPGAVYSFRTRLKRLL
                     ACFRAQALQSYDAPSAPPANLSSGGVD"
     gene            1296..2051
                     /locus_tag="VFQ35_02840"
     CDS             1296..2051
                     /locus_tag="VFQ35_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929591.1"
                     /translation="MDRDPEELLRELGTLHAARADELARAQPDDASPSLWGPLSEAES
                     RAVIAAALGRLNAESCVVPVGPKSASRARRHGRELVVLAFAAVAAGVLLLLSTPRALP
                     RYEISSPNSDSVARDVSNVTEPRRAARAGDPKHYELGRELRFVLRPAHAVKGPVRVSL
                     FSTRGAIRSLPLAQIEQLPGGSVAGTLLTGPSGYAPVPGPDTLVFAIGVETPRSADVE
                     RGVSTSSLQLFRLNVVWHAANGEGKTDGAGERR"
     gene            2029..>3704
                     /locus_tag="VFQ35_02845"
     CDS             2029..>3704
                     /locus_tag="VFQ35_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929592.1"
                     /translation="MARESAAESSSNEDPRRRLSTWPALLFALTAAALPPLDTPPTHV
                     VSGMAISGCSAVVELDVCELPREQVRTISVWIPRTGAKRAVTLRLVGATQSAPTRYTE
                     LASGTRIDASLRLDAAEGSLTVATGTDLVTRVGLKPSPVWRWQSQVAAASASETLSRL
                     APLLAGTVGAEHSQMARAVAESAIGLGEHRRGFEVLADGARVALAARRYSEARSHLLR
                     LAFHQSHAHDFAAAETALERARAVPSSRMLDADGEAAIAYVSAVEAANVRDLRRAAQA
                     YEDGQSWVERGAVLMAPQIAMGSALIAEEQGRLRDALALLESALKSKLGTASPCFRAL
                     ATTRAGWYALELYPRDDVSPGVVAARERLRQASAIYKSECTKAPPAEPAAAGLYLGFA
                     ALRFGELEEARAELARAGEFASSPRLRLWANLLGAEIHLAQGRVEEAERSFRELELRA
                     QGLLSGDLAWRASEGLARTFVARHWLEAAVTEFRRAESLLISSIEWVSIGAGALQFLD
                     AHREPTEGLAATLIAVGRISEGYFALRRARRRLVVELARNLNATESSRGR"
BASE COUNT          589 a         1185 c         1272 g          658 t
ORIGIN      
        1 cctctcgaaa tcgaggcgct gagtcgcgtg ttcgcggctc gcttctgcgc ggagcacggc
       61 cgggcgcgct tgaacatcac cgccgagtgt ctgcgcgcgc tggagcggta ctcgtggccg
      121 ggcaacctgc gcgagctccg caacgcgatc gagcgcgcgg tggtgttgtg tcagggcgac
      181 gctctcttac ccgagcacct cccgcccaag gttcgcgccg gcaagcccac cgacatcgct
      241 tcggagccgc ccggagaccg ccgcgcgctg ctccagaagg aattcgacga ccttgagcga
      301 acgcggattc aggccgcgct cgacaaggca ggtggcaatc agacgacggc cgccaaactc
      361 ctcggcatct cgcggcgcac gctcgtgtat cgcctcaccg cgctgcagct gccgcgacct
      421 cgaaaaggct agaccaagcc cgcgcgagtc gagcaaagcg ttcacgagcg ttcaggcttg
      481 aaaaatctca ctcgcccgct ggcgaaagcc gcgtgtcgat gactgaaaga agccgccccg
      541 ctgtcccgta ccgcaatgac tatggaagtc gcgctcgtct cgatggtaac tcgagcagag
      601 ggcactcagg ggaaccctgg cgtgaagcac gacaccgttc atgaagatct ggagctcctg
      661 cggcgcgttc gttcgggaga accctctgct cgcgaggcgt ttctcgttcg agtcaccgat
      721 gtagtgcgag tccgcgtcgg acgcgtgctg gcgcgcgctt cgcgcaaccg cgcacaggcc
      781 gatttgcgcc gtcgagatct gctcgatctg atgcaggacg tattcgttgt gctgttggac
      841 cgagacgcga aggtgctcgc ggcctgggaa cccgagcgtg gactatctct cgacggcttc
      901 gtgggcctcg tcgctgagcg cgagaccttg gccttcttgc gcagcgggcg acgaagcgca
      961 tgggccgagc atccgagtga ggacatcgtc gacgaggggg tggaggacgt ctccccagag
     1021 ctgcaggctt cggccaagga agagctgagt ctcctgctcg actatctttc cgaccgcctg
     1081 agcccgcagg gcgcactgct ctttcaagct ctgtacgtcg atcatgcctc gctcgaagac
     1141 gtgtgcgaac gcttttccat gagcccgggt gcggtgtaca gtttccgaac tcgcttgaag
     1201 cgcctgctcg cgtgctttcg agcgcaagct ctgcagagtt acgacgcccc ttcagccccg
     1261 cccgccaatc tttcctcggg gggcgtagac tgaccatgga tcgggatccg gaggagctct
     1321 tgcgcgagct cggaacgctc cacgcggcgc gtgccgatga gcttgcgcga gctcaacccg
     1381 atgatgcttc tccctcgctt tggggtcccc tctccgaggc ggagtcgcgc gccgtcatcg
     1441 cggccgcgtt gggtcggctg aacgccgagt cctgcgtggt cccggtgggg ccaaagagcg
     1501 cgagccgcgc tcgaagacac gggcgcgagc tcgtcgtgct tgccttcgcg gcggtggcgg
     1561 cgggagtgct ccttcttctg agtacgccgc gcgcccttcc tcgatacgaa atttcgagtc
     1621 cgaactccga ttccgttgcg cgtgacgtgt ccaacgtgac cgagcctcga cgcgcggcac
     1681 gagctggaga ccccaagcac tacgaactcg gacgcgagtt gcggttcgtg cttcggccgg
     1741 cgcacgccgt aaaggggccg gtgcgcgtga gtctcttctc cacccgtggc gcgattcgtt
     1801 cgctgccgct cgcgcaaatc gagcaactac cgggtggcag cgtggcgggg acgttgttga
     1861 cggggccctc gggctacgcg ccggttcccg gcccggacac gctcgttttt gcgatcgggg
     1921 tcgagacgcc tcgaagcgcc gacgtcgagc gcggtgtctc gacttcgtcg ttgcagctct
     1981 tccggttgaa cgtggtttgg cacgctgcga acggggaggg gaagacggat ggcgcgggag
     2041 agcgccgctg agtcttcatc gaacgaggac cctcgccgac gtctctcgac gtggccagcg
     2101 ctcctatttg cgctgacggc ggcggcgctg ccaccgctcg acacgcctcc gactcatgtg
     2161 gtctcgggga tggcgatatc tggatgctcg gcggtcgtgg agctcgacgt ttgtgagttg
     2221 ccgcgggaac aggtcaggac gatctcggtg tggataccac gcaccggcgc gaagcgagct
     2281 gtgactttgc gtttggtcgg agcaactcag agcgcgccca cgcgctacac cgagcttgcc
     2341 tcgggaactc gcatcgatgc gtcattgcgt ttggatgccg cagaaggcag cctgacggtc
     2401 gcgacgggca ccgacctggt gacgcgcgtc ggtttgaaac cctcgccagt ttggcgctgg
     2461 cagtcccaag tcgccgctgc ctctgcgagc gaaacgctct cccgactcgc cccgctgctc
     2521 gccggaaccg tgggggccga gcactcgcag atggcgcgag ccgtcgccga gtcggccatt
     2581 gggctcgggg agcacaggcg cggcttcgag gttctcgcgg atggcgcacg cgtggcgttg
     2641 gcggcgaggc gctactccga agcacggagt cacttgctcc gcctcgcttt tcatcagagt
     2701 cacgcccacg atttcgcggc cgcagagacc gcgctcgaac gtgcgcgcgc ggtaccctcc
     2761 tcgcgcatgc tcgacgccga tggggaagcg gcaatagcgt acgtgagcgc ggtggaagcc
     2821 gcgaacgtgc gcgatctcag gcgcgcggcg caagcatacg aagacgggca gagctgggtg
     2881 gaacgaggag cggtgctgat ggcgcctcag atcgcgatgg ggagcgcctt gattgcggaa
     2941 gagcaagggc gccttcgcga cgcgctggcg ctgctggaat ctgcactcaa gagcaagcta
     3001 ggcacggcgt cgccatgttt tcgtgcgctc gcaacgacgc gagccggctg gtacgcgctg
     3061 gagctctacc cgcgcgacga cgtgtcgccc ggcgtcgtcg ccgcacgcga gcggctgcgg
     3121 caagcgtctg cgatctacaa aagcgagtgc accaaggcgc cgcccgcgga gcctgcagcc
     3181 gcagggcttt acctcggctt tgcggcgctt cggttcggcg agctcgaaga ggcgcgcgcc
     3241 gaactcgcac gcgcaggcga gttcgcatcg tctccgcgac ttcgcttgtg ggctaacttg
     3301 ctcggcgccg agatccactt ggcgcagggc cgcgtcgaag aagcggagcg ctcatttcgc
     3361 gagctcgaac ttcgggcaca ggggcttctg tccggggatc tcgcgtggcg ggcgagtgag
     3421 ggcctcgcgc gcacgttcgt ggcgcggcac tggctggagg ctgcggtcac cgagtttcgg
     3481 cgggcggagt cactgctcat aagttcgatc gaatgggtct cgatcggcgc tggcgcgctt
     3541 caatttctgg atgcacatcg ggagccaacc gagggattgg cggcgacctt gatcgccgtg
     3601 ggtcggatct cggaaggcta cttcgcgctg cgtcgagctc gacgaaggct cgtggtcgaa
     3661 ctcgcgcgca atctgaacgc aaccgagagc tcgcgcgggc gaaa
//
LOCUS       DASTKS010000134         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_912921, whole genome shotgun sequence.
ACCESSION   DASTKS010000134 DASTKS010000000
VERSION     DASTKS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912921"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFQ35_02850"
     CDS             <1..681
                     /locus_tag="VFQ35_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9929593.1"
                     /translation="SPPVSERLPLADLWAGVRAGTHFVQEGYYSEERCFLGIELRPTP
                     SPRRKPLRMDVFERILSGEGYKRVAFEDKLAVSTISVLCGDCLDAIGAARTTSRVPLL
                     LLMAVHAHRGVSLPPARMYRWRNDGKEQWVVTAERPDRTLPRDLTGSEASVVRSLVEG
                     LSHQQIGELRSTSRRTVANQLAQAFRKLGVSGRTELIAMLVRRGASVTLSPPLEAEMP
                     SLSGPAPW"
     gene            780..1412
                     /locus_tag="VFQ35_02855"
     CDS             780..1412
                     /locus_tag="VFQ35_02855"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET9929594.1"
                     /translation="MERVRARTELVEALSRESGVTDERILNAFARVPRHLFVPQNADD
                     SAYSDVALPIGFRQTISQPSMIAIMLDALAIRPDDRVLEIGGGSGYAAALMAELGAEV
                     FTLEIVPELAARATECLSALGYERVHVVNANGREGLPEHAPYDVVMVSAAASDVPDEL
                     VRELAPGGRIAIPVGGTDEQELLVGRKDSRGAVHWQRSVPCVFVPLVGDA"
     gene            complement(1492..2052)
                     /locus_tag="VFQ35_02860"
     CDS             complement(1492..2052)
                     /locus_tag="VFQ35_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET9929595.1"
                     /translation="MSVLAPGFLVAAPPLGDPNFDRSVVLVAAHGDEGAFGWIINGRE
                     VMTLSELLVQADVSQSPPPLAGSVRVGGPVSPEQVWLVYREQHRFRDIDEQFDVGPGV
                     VACASRRVLEAIARGDAPPSILGLVGYAGWAPGQLENEIRVGAWLPVDLDVTLLFDEP
                     RDTLWQRAYERAGTTPMAFTSRVGLA"
     gene            2229..>2762
                     /locus_tag="VFQ35_02865"
     CDS             2229..>2762
                     /locus_tag="VFQ35_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9929596.1"
                     /translation="MHVTFYGVRGSVPAPGPLTARYGGNTSCVEVRLADGTILALDAG
                     TGLRTLGNTLVREGRQAQVHLLLSHTHWDHVLGLPFFAPLWNKANHIIVYPLANDAQE
                     RFQRNIFDDIHFPVSVNDIPAKLELAKPAGAAWRIGSAMVRPVQLNHPGGAQGFRIDD
                     DDGSSLAYLTDNELNAAS"
BASE COUNT          484 a          952 c          882 g          444 t
ORIGIN      
        1 tccccgcccg tatccgagag attgccgctc gcggacctct gggccggcgt ccgcgcggga
       61 acgcacttcg ttcaagaagg gtactactcc gaggagcgct gctttctcgg gatcgagctg
      121 cgcccaacgc cgtctccccg acgcaaaccg cttcgcatgg acgtgttcga gaggatattg
      181 agcggcgaag gctacaagcg cgtcgccttc gaagacaaac tcgcggtctc gacgatttcc
      241 gtcctatgtg gagactgcct ggacgcaatc ggtgcggcac gcaccacctc acgggtaccg
      301 ctgctgctcc tcatggccgt tcacgctcac cgcggcgtct ccttgccgcc ggcgcggatg
      361 taccgatggc gcaacgacgg caaagagcaa tgggtggtca ccgctgagcg ccccgaccgt
      421 acgctgccgc gcgacctcac cggatccgag gcctcggtcg ttcgctcgct cgtcgagggc
      481 ctcagccatc agcagattgg cgagctccgc agcacctcac gtcgaaccgt cgctaatcag
      541 ctggcgcagg cgttccgcaa actcggtgtc tccggacgca ccgagctcat cgccatgctc
      601 gttcgacggg gggcgagcgt tacgctttcg ccaccgctcg aggcggaaat gccgagcctc
      661 agcggtcccg cgccctggtg atcgcgccca tctctgttgc caaacgctcg gcgcggaccc
      721 actctgttcc ttcgatggga ctcaccgagc gccgcgcgca gaccgatccg cctcaaagca
      781 tggagcgagt tcgcgcgcgc accgagctcg tcgaagcgct gagtcgagaa agcggcgtta
      841 ccgacgaacg catcctcaac gctttcgccc gagttccgag gcacctgttc gttccgcaaa
      901 acgccgacga cagcgcttac agcgacgtcg ccctcccgat cggcttcagg cagaccataa
      961 gccaacccag catgatcgcg atcatgctcg acgcgctcgc gattcgcccg gatgatcgcg
     1021 tcctcgaaat cggtggcggc tccggttacg ccgctgcgtt gatggccgag ctcggcgcgg
     1081 aggtgttcac actggagatc gtgcccgagc ttgccgcgcg cgcgaccgag tgtctcagcg
     1141 cgctcgggta tgagcgcgtc cacgtcgtga acgcaaacgg ccgcgagggc ctccccgagc
     1201 acgcgcccta cgacgtggtg atggtctccg cagccgcgtc cgacgtcccc gacgaactcg
     1261 tccgggagct cgcgccgggg ggtcgcatcg cgatccccgt cggcggcacg gatgagcaag
     1321 agctactcgt cgggcgcaaa gactcgcgtg gcgccgtcca ctggcaacgc tcggtcccct
     1381 gcgtcttcgt tccgctcgtc ggcgacgcct gatcgtcggc cactcggcac cagcgggctg
     1441 cgacatcgag cgctgtgaat cggacgcgcg agagcgaatc agtgaacggg atcaggcgag
     1501 gccgacgcgc gatgtgaacg ccatcggcgt ggtgccggcg cgctcgtagg cccgttgcca
     1561 aagagtatcg cgcggctcgt cgaacagcag ggtcacgtcg agatcgacgg gcaaccaagc
     1621 accgacgcga atctcgttct cgagttgccc cggcgcccag ccggcgtacc cgaccaaacc
     1681 caggatgctc ggcggcgcgt cgccccgtgc gattgcttcg agcacgcgtc gagaggcaca
     1741 cgcgacgacc cccggcccga cgtcgaattg ttcgtcgatg tcacgaaacc ggtgctgctc
     1801 gcggtaaacg agccacacct gctccggaga caccggtcct cccacgcgca cactgccggc
     1861 gagcggcggc ggagactggg agacgtccgc ctgcaccaaa agctcggaca gggtcatgac
     1921 ctcgcgaccg ttgatgatcc agccgaacgc gccttcgtct ccgtgcgcgg cgacgagcac
     1981 gaccgagcgg tcgaagttcg gatcaccgag cggcggagcc gcgacgagga agccgggggc
     2041 aaggactgac atcgcgggtc cctcgagtat agtcgctctt cccgcgccgc gtcacgccgc
     2101 tcgggagcgt attgccgccc cgccggccgg gcaccgcgat cggcaccgag aacaggctag
     2161 ccgcgcggga acggggaaga gcggggaccg tttgtcgtcc tacctcggtt tggatagagt
     2221 gaggagcgat gcacgtgacg ttctatgggg tccgcgggtc cgtacccgcg cctggaccac
     2281 tgactgcccg ctacggaggc aatacgtcat gtgtcgaggt acggctcgca gacgggacga
     2341 tcttggcgtt ggatgccggc acggggctgc gcactctcgg aaacacgctc gtgcgcgagg
     2401 ggcgacaggc acaagtgcac cttctgctgt cgcacacgca ctgggatcac gtgctcgggc
     2461 tgcccttctt cgctcccctt tggaacaaag ccaatcacat catcgtctat ccgctcgcga
     2521 acgatgccca ggaacgtttc cagcgcaaca tcttcgacga cattcatttc ccggtcagcg
     2581 tgaacgacat cccggcaaag ctcgagctcg caaagccggc gggagcagcg tggcggatcg
     2641 gtagcgcgat ggtgcgcccg gttcagctca accatcccgg gggtgcccaa ggcttccgca
     2701 tcgacgacga cgacggctcg tcgctcgcat acttgactga caacgaactg aacgcggcgt
     2761 cc
//
LOCUS       DASTKS010000135         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1806101, whole genome shotgun sequence.
ACCESSION   DASTKS010000135 DASTKS010000000
VERSION     DASTKS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806101"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..867
                     /locus_tag="VFQ35_02870"
     CDS             187..867
                     /locus_tag="VFQ35_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929597.1"
                     /translation="MMGRHSHRRWALAVLVAASCAKREPSVGSYPLRLVVTNVPGEPL
                     SGVAARIDGRSLGKTDEQGVLALTLRGRDGQRLNLHLECPPNHKPPKAPLEVALFRYA
                     AGSTPELTAVCEQETVAEAVVVRTPGARDVPLYARGERVGQTDAQGVAHLLLRGAPGE
                     NVDLAFDTSERPELRPASPSVRVTLANQDDVLLAEQRFEQRPKPRVKASTVPRAGTTP
                     VKGPVRIR"
     gene            909..1115
                     /locus_tag="VFQ35_02875"
     CDS             909..1115
                     /locus_tag="VFQ35_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9929598.1"
                     /translation="MLQRIRDIVEKNRFARDTRGASSVEYLIVVGLIAFVVFASYQKF
                     GNNVKTKVDSAATTVDGMTIGSGP"
     gene            1336..1509
                     /locus_tag="VFQ35_02880"
     CDS             1336..1509
                     /locus_tag="VFQ35_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929599.1"
                     /translation="MQTKSILSLSKSERGQITIEYLVTAAAVSLIVYLSCSNFGQATI
                     AGLKLAARDLLGR"
     gene            1521..2315
                     /gene="cpaB"
                     /locus_tag="VFQ35_02885"
     CDS             1521..2315
                     /gene="cpaB"
                     /locus_tag="VFQ35_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9929600.1"
                     /translation="MPQSKALIVGLVAGVTAGALGWMYMERFESDVSGGTQVSVLTTS
                     KDIAPGTEVLPSMLAVRHVPAAYVDDRAVLARDQQKVVGIEVVTPLKAQEVLQWTDLA
                     VRSDARNVSQLVAPGKRAMTIRAKASDNAGNGLVQPGDYVDVFAVVDGHGDRTSALLL
                     QRVLVIAVGNETEQARDGKPVDKGRRDERLLTLSLDVDEAQLLSLAAEQGRLAVALRN
                     PTDQRVFDDMPDLGASALVAARDKVQARKSRVRVPASAPVKLTEAR"
BASE COUNT          463 a          804 c          828 g          394 t
ORIGIN      
        1 cccaggcgcg acctcgagaa atcacgctgc tgcgcgcgtg gagggggcgt ccggtgccaa
       61 accgcgtttg gcagtcgcgg cttgcggtcg cggcccacgc aagcggcgct ttgcggttcg
      121 tgcggcgcaa attcgagcca atccgcgcgc ttacggagtg gcactcggct tgctctgggc
      181 gaaggcatga tgggccgaca tagccaccgc cgctgggcac tcgccgtgct cgtcgccgcg
      241 agttgcgcaa agcgcgagcc atccgtcggc agttacccac ttcgtttggt ggtcacgaac
      301 gtaccaggcg agccgctctc gggagtcgca gctcgcatcg atgggcgctc gctcggcaag
      361 acggacgaac agggtgtgct cgccctcact ctccgcggcc gcgatgggca gcgcctgaac
      421 ctgcacctcg agtgcccacc caaccataag ccacccaaag caccgctcga agtcgcgttg
      481 tttcgctacg ccgccggttc gactccggag cttaccgccg tgtgcgagca agagactgtc
      541 gcggaagcag tggtggtgag aacgccgggc gcgcgcgacg ttccgctcta cgcgcggggt
      601 gagcgggttg ggcaaacgga tgcgcaaggc gtcgcccacc tgctgctgcg tggcgcgcca
      661 ggcgaaaacg tcgacctcgc cttcgacacc tccgaacggc ccgagctgcg acccgcgagc
      721 ccgtcggtgc gcgttacgct cgcgaaccaa gacgacgtgc tgctcgcgga gcagcgcttc
      781 gagcagcgcc ccaaaccgcg cgtgaaggcg agcacggtac cacgcgccgg gacgacgccc
      841 gtcaaaggac cagtccgtat tcgttgaaca cggatacgag caggaacgca caacgaacag
      901 gagatcgcat gcttcaaaga attcgcgaca tcgtcgaaaa gaaccgattc gcccgtgata
      961 cgcggggcgc gtcgtccgtc gagtacctca tcgtagtcgg cctcatcgcg ttcgtcgtat
     1021 tcgcctcgta ccaaaagttc ggcaacaacg tgaaaaccaa ggtggacagc gccgcgacca
     1081 cggtcgacgg catgaccatc ggcagcggtc cataacggcc gagggcgtac gcgatcgagg
     1141 caagcgcgca gccggagctc acatgctcac acgcatcgct ccggtgttcg caaaagcccg
     1201 gccaaagccc gagactgggc ggggggctcg tgcttctgcg cgtcaggcgc gggcctcgat
     1261 cgcgattcac tcgaacctgg gggtgacagg cggtctcgcg tgagcggtca cgtgagccac
     1321 aggagcaaat gggtcatgca aactaagtcg atattgtctc tctcgaagag cgagcgcggt
     1381 caaatcacga tcgagtatct ggttaccgcc gccgccgtca gcctgatcgt ctacttgtct
     1441 tgctcgaact tcgggcaagc caccatcgcg gggctgaagc tcgccgcgcg tgacctcttg
     1501 gggcgttgag gagccgcgcg atgcctcaat caaaagcgct catcgtcggt ctggtcgcag
     1561 gcgtcaccgc cggcgcgctc ggctggatgt acatggagcg gttcgagtcg gacgtatccg
     1621 gcgggacaca agtctcggtt ctcaccacga gcaaagacat cgcgcccggg accgaggtcc
     1681 tgccgagcat gctcgccgtt cgtcacgttc cagcggctta tgtcgacgac cgcgccgttt
     1741 tggcgcgcga tcagcaaaag gtcgtcggga tcgaggtggt gacaccgctc aaagcgcaag
     1801 aggtgctcca gtggacggat ctcgcggtgc gcagcgacgc gcgcaacgtg agccagctcg
     1861 ttgcgccggg caagcgcgcc atgaccatcc gcgcgaaagc ttctgacaac gccggcaacg
     1921 gcttggtcca gcctggcgac tacgtcgacg tgttcgccgt ggtcgacggg cacggcgatc
     1981 gcacttcggc gctgctgttg cagcgcgtgc tcgtgatcgc ggtcggcaac gagaccgagc
     2041 aagcgcgcga cggcaagccg gttgacaaag ggcggcgcga cgagcggctc ctgactttga
     2101 gcctcgacgt cgatgaagcg cagttgttgt cgctcgccgc ggagcaaggg cgtttggcgg
     2161 tggcgttgcg taacccgacc gatcaacgcg tgttcgacga catgccggac ctcggcgcgt
     2221 cggcgttggt cgcggcgcgc gacaaagtgc aagctcgcaa gagccgcgtc cgcgtgcccg
     2281 cgagcgcgcc cgtgaagctc acggaggcgc gatgaaagcc gtgaagtcac tcgcgacggc
     2341 gctgctcgga gcgctgttcg ttaccgccag cccgagcctc gcccaaggcg aaaaaggccg
     2401 ggaattcacc caaaccgaga accgcgacgt gagcttgtcg gtgggtgaga atcagaccat
     2461 ccccgcgagc gacgtgaaga gctactccg
//
LOCUS       DASTKS010000136         8645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_243452, whole genome shotgun sequence.
ACCESSION   DASTKS010000136 DASTKS010000000
VERSION     DASTKS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243452"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..637
                     /locus_tag="VFQ35_02890"
     CDS             44..637
                     /locus_tag="VFQ35_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9929601.1"
                     /translation="MKVTRRAARLSRKHERLALPSEGALRLGVVADTHSSPHPRTLEL
                     LRTVRPAAILHAGDIGDFAVLRDLATVAPVIAVRGNIDPIDAAAPDVFVLDLGNGELP
                     VLRVLLTHIAVNGPVLRADAARLARDEKAGLVICGHSHVPFIGRDRGIAVFNPGSVGP
                     RRFMLPIVFGLLEVSPVTTRMSHIDCETGQPWFPPAF"
     gene            742..1755
                     /locus_tag="VFQ35_02895"
     CDS             742..1755
                     /locus_tag="VFQ35_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET9929602.1"
                     /translation="MSTKVTLIRGDGIGPEVVDAAVRAIDATGAAIQWEPHEAGAGAI
                     PKHGAPLPESTVASIRENRWCLKGPLATPIGGGYRSVNVALRQEFDLYANLRPARTYE
                     GIKSRYDHVDLVVVRENTQGEYSGIEHYIDPSRSAAEAISIITRHGSDRILRFAFEFA
                     RANQRKRVTLVHKANILKLTSGLFLETGREIARSYGDIEFNDIIVDNCAMQLVRDPTR
                     FDVIVTTNLFGDILSDLTAGLVGGLGLVAGANIGHEASIFEAVHGTAPDIAGKGIANP
                     TAVMLAGAMLLDRMGERARAQRLELSIRRVIAEGRDTTPDIGGNGTTTSFTERVISAL
                     AHS"
     gene            1940..3268
                     /locus_tag="VFQ35_02900"
     CDS             1940..3268
                     /locus_tag="VFQ35_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929603.1"
                     /translation="MSTPDTPSGPPPPPRRGGSASTPPAADPVKAESASDPSISVDAS
                     TPSEPATRDSEPDSGPDSDALPTIVRSEPPMLSMMSLVAHGEQREAALPANPPLPSAQ
                     VTSSPPPPEEAPESATVVQRPGPQEKKGPTRSRDGLRAIGQDVQGAIARAIEYLDVRD
                     GWVPIRWRGRVRGSVLVLSGALLTIAVIGGVVAIVKASSSARSSDVVASAPSVETPAT
                     PAVFAATPAPTAAPVKEVEVKTAPPAAPRSTLATAPMSESDEGALLLESARSFTWQGR
                     DSEAVTMVERALLRNPELRTDARVREVLLRTAHSDDKATSAATFALLEGPMVETGAEL
                     MYEISLARVGKEAARKRAQSFLRSKQFAKNAALPLFIAVRLRQAKSCEDKHALLTLAQ
                     GGGKQTLDYLRELQAHTACTPDDLVNCYPCLRADGKLSEAIAAIEKRTKG"
     gene            3281..4060
                     /locus_tag="VFQ35_02905"
     CDS             3281..4060
                     /locus_tag="VFQ35_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929604.1"
                     /translation="MRAAWLSSLALLWFGCAPPSARPESEAVAERVSPARVDLSRIRL
                     GPGVTESTLELAGGGARSVLLWQPEGEPNSVIVFLHGAVPVGAPRPWRAPAQTRGLVE
                     CLAVPAFAALRPIILLPRSERGEWWSRADAEFVLGLLEAAVQRWPTAAHRKAIVGYSN
                     GGIGTWYFARAYPTYFDAAIPMASNDTVVGATRLPVFAIHGENDELFSIERVRSAIEA
                     LKSEGQDVTLLAKYRGTHLAPCGYVDELGTAARWLSSHVWH"
     gene            complement(4167..7016)
                     /locus_tag="VFQ35_02910"
     CDS             complement(4167..7016)
                     /locus_tag="VFQ35_02910"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9929605.1"
                     /translation="MSRSRVVGFVLFALTGCAAPAPRSAPSPVVAPPTSALATAPVDE
                     LDKALPLDARLTKGRLDNGLTYYVLAHHKPEHRAQVWLVVNAGSVLEDEDQRGLAHFT
                     EHMAFNGTRRFPERALTDFLEKSGVRFGADLNAYTNFDETVYMLQVPTDNPELFKQSF
                     SVLRDWADGISFDPKEVEQERGVVLEEWRLGRGAGMRLFDKQAKVVFAGSKYADRITI
                     GKPEIIQHASRDTLVRYYRDWYRPDLMAVIAVGDFAPSDAEAQIKAEFSTLAPAKSPR
                     PRPVVTLPSHPDTLVSVETDPEMPAASVSILSEFPHRPEASARDYRRKIGEHLFNTML
                     NERFEELSRKPDAPFLGANSSTSGLTRSTDVFRQSAAIRDDAALTGFSALLEEVLRVE
                     RHGFTQGELERAKSQLLRRFQHAVKERDKTDSRDFAAEISRNFLQEETMPGAEAELAL
                     VERFLPTFSLPELNQLGKTMGAGSRVILVNGSEHLKKPHPEALLALSHELEATQIAAY
                     SEANQDLTLMAQNPAPGSVLKTSTVADLDITRWTLKNGVEVIAKPTSFANDDVRLTAF
                     SPGGSSLVKDADFASARFADSVVALGGLGAFDAIQLRKALAGKLASVTPHIGELDEGL
                     SGRASSGDLELLFQLTYLTMTAPRRDESTFNAWRAREVERTSHRLLSPELTFFEELQT
                     LTSQNHSRRRPVTPDLLQQIDLDKAFAIYRDRLGDASDFTFVFVGNFSLDELKPLAET
                     YLGSLPARGRKEKWRDTHVTWPRGIQRKVVVKGHEPKSLVTLNFHGKQPFSRDAADDL
                     EILQDVLRHRLREILREDMGGVYGVQVNGRIARRPREEYTLSINFGCQPENVDKLTSA
                     VFSAISELQQRGIADDYLLKVKEERRRAHETELLDNGYWLHELERVYTFGDDPHTIVD
                     LSKELERATSERVRASAKRYLDTKEYVLGVLKPEG"
     gene            complement(7060..7383)
                     /locus_tag="VFQ35_02915"
     CDS             complement(7060..7383)
                     /locus_tag="VFQ35_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929606.1"
                     /translation="MLERLRHKWQRLKQAPPGERFERFHDEERNRPAWVRVIYLVLAI
                     ALIPVGVLFAFIPGPAVLFFALSAGLFAVQSAWVAARLDRLEVAIRSQIRRLRAAYRR
                     RHPAH"
     gene            7521..8192
                     /locus_tag="VFQ35_02920"
     CDS             7521..8192
                     /locus_tag="VFQ35_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929607.1"
                     /translation="MVEALSGVADRGCLYVSAVLGLCGCSTLTQISAGPTFLPSSERD
                     SRAGAEVLARAASGFGSEKGFGGIEAVGRAFALGEQQGFSAGLGPAWVQTWGKGWLMF
                     DADAMLGVAYTKHEPWVLGTFRAGLGGGVELSSSTSARPHRLLFGGEPMPGLVEIFHD
                     ATALTIDLGPAVDVSGGQSPAFSLGVRIGIAWIELRYTDRDPLDAFTPYGRGRIPFRA
                     MPAPR"
     gene            complement(8203..>8645)
                     /locus_tag="VFQ35_02925"
     CDS             complement(8203..>8645)
                     /locus_tag="VFQ35_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9929608.1"
                     /translation="VAFAEPEEIDRLNIYWAGLAAMRRAVEMLGLVPEHLLIDARKLR
                     DVPIEQTRIVKGDERSLSIAAASIIAKTERDARMLEYDQEYPHYGFARHKGYPVREHV
                     AALKKHGACPIHRRSFAPVRSALGLPPLPKPESRAKVAPRRKKS"
BASE COUNT         1440 a         2714 c         2885 g         1606 t
ORIGIN      
        1 agcgcgcgtg gacgagcggc actcgagctc gtagattcgg cggatgaagg ttacgcgccg
       61 cgccgctcga ttgagccgca agcacgaacg gctcgcgctc ccctccgaag gggctttgcg
      121 cttgggcgtg gtcgcggaca ctcactcgag cccccatccg cgaaccctgg agcttctgcg
      181 taccgtccgt cctgccgcga tccttcacgc tggtgatatc ggtgacttcg cggtgctgcg
      241 cgatttggcg acggtcgcgc ccgtcatcgc cgtgcgcggt aacatcgacc cgatcgatgc
      301 agcggcgcca gacgttttcg tcttggatct cgggaacggc gaattgcccg tgcttcgagt
      361 cctgctcacg cacatcgccg tgaatgggcc ggtgctgcgc gccgacgccg cacgactcgc
      421 tcgcgacgag aaagctgggc tggtgatctg cggccactca cacgtgccgt tcatcgggcg
      481 cgatcgaggt atcgcggttt tcaacccggg ctcggtcggc ccacggcgct tcatgctgcc
      541 gatcgtgttc ggcctgctcg aggtgagccc cgtcacgacg cgcatgagcc acatcgactg
      601 cgagaccgga cagccctggt ttcctcctgc tttctagcca tgaagacctg cggtgcagag
      661 tggcgactct gcgcgctgtg gcgagtttgg ccaactccga gatgaatacc gtgtgctcga
      721 gaggcggtgt acgatgcgcg catgtcaacc aaggtcacgt tgatccgagg ggatgggatc
      781 gggcccgagg tcgtggatgc cgcggtgaga gccatcgacg cgacgggcgc cgccattcag
      841 tgggagcctc acgaggccgg cgcaggcgcg attccaaagc acggcgcgcc cttaccggag
      901 agcaccgtcg catccatccg tgaaaaccgt tggtgcctga aggggccgct cgccacgccg
      961 attggtggcg gctatcgcag cgtcaacgtg gcgcttcgcc aagagttcga cctttacgcc
     1021 aatcttcgcc ccgcgcgcac gtacgaggga atcaagagcc gctacgatca cgtcgatctc
     1081 gtggtggtgc gtgaaaacac gcaaggcgaa tactccggga tcgagcacta catcgacccg
     1141 agccgaagcg ccgccgaagc catctcgatc atcacgcgcc acggttcgga tcgcatcctc
     1201 cgcttcgcct tcgagtttgc gcgtgcgaat caaagaaagc gcgtgacgct cgtgcacaag
     1261 gccaacatcc tcaagctcac ttccggcctg ttcctcgaaa cggggcgtga gatcgcgcgg
     1321 agctacggcg acatcgagtt caacgacatc atcgtcgaca actgtgccat gcagctcgtg
     1381 cgcgatccga cgcgcttcga cgtgatcgta acgaccaacc tcttcggcga tatcctgtcg
     1441 gatcttacgg ctgggctggt tggtggcctg ggtctcgtag cgggggcgaa catcggccac
     1501 gaagcgtcga ttttcgaggc ggtccacggc accgctccgg acatcgctgg caaggggatc
     1561 gctaacccca ccgccgtcat gctcgcaggc gcgatgctcc tcgatcgaat gggtgagcgc
     1621 gcaagggctc agcggctcga gctctcgatt cggcgcgtca tcgccgaggg gcgcgatacg
     1681 acgccggaca tcggcggaaa cggaacgacg acgtccttta cggagcgcgt catttcggcg
     1741 ctagctcatt cgtagactcg cactgaacgc gctggcgcgc agtgaggcgc ggctgtcctt
     1801 ccaactcggc tcgaactgag gcaagttgca gtttgaatcg gagttttcgc gatcaggcgg
     1861 aattggttca aataactttg ctgacgaact ccatggtttg cgagctcgac ggctcgggac
     1921 tgcactaaac tcgctcgtca tgtctacgcc cgatacgccc tccggccctc cgccccctcc
     1981 gcgtcgaggt ggaagtgctt cgaccccgcc ggctgcagat cccgtcaagg ccgagagcgc
     2041 ttcggaccct tcaattagcg tggatgcttc gacaccgtct gaaccggcaa ctcgcgactc
     2101 ggagcccgac tcgggcccag acagcgacgc gcttccgacc atcgtgcgca gcgagcctcc
     2161 gatgctttct atgatgagct tggttgcgca cggcgagcag cgcgaggcag cgctcccggc
     2221 gaaccctccg ttgccgtctg ctcaagtcac gtcgtcacca ccgccgcccg aagaagcgcc
     2281 ggagtcagcc acggtcgtgc aacgccctgg gccccaagag aagaagggac cgactcgctc
     2341 ccgggatggt ttgcgagcca ttgggcagga cgtgcagggc gcgatcgcgc gcgcaatcga
     2401 gtacctggac gttcgcgacg ggtgggtgcc cattcgttgg cgagggcggg ttcgaggttc
     2461 ggtgttggtg ctctcgggcg ccttgctgac gattgcggtg atcggaggag tcgttgcgat
     2521 cgtcaaggcg agctcttctg cgcgatcgag tgacgtggtt gcctctgccc ccagtgtcga
     2581 gacgcccgca acccccgccg tgtttgcggc caccccggcg ccaacggctg cccccgtgaa
     2641 ggaagtcgaa gtaaagactg ctccgcctgc ggcgcctcgc tccactctgg ccaccgcgcc
     2701 gatgagcgaa agcgatgaag gcgcgctgct gctcgagtcg gcgcgctcgt tcacttggca
     2761 agggcgcgat tcggaagcgg tgacgatggt cgagcgtgcg ctgctccgga acccggagtt
     2821 acgtaccgac gctcgcgtac gcgaagtgtt gctgcgtacg gctcactcgg acgataaggc
     2881 tacttcggcc gcgacgtttg ctttgctcga ggggccgatg gtagagaccg gcgcggagct
     2941 gatgtacgag atctcgctcg cgcgggtcgg gaaagaagcc gcgcgaaaac gagcgcagag
     3001 cttcttgcgc agcaagcagt tcgccaagaa cgcggcgttg ccgctcttca tcgccgttag
     3061 gcttcgccaa gcgaagagct gcgaagacaa gcacgccttg ttgaccttgg ctcagggcgg
     3121 cggaaagcag acactcgact acctgcgaga gttacaggct cacactgctt gcacgcccga
     3181 cgacttggtc aattgctacc cgtgcttgcg cgccgatggc aagctgagcg aggccatcgc
     3241 ggcaatcgaa aagcgaacca aaggctgaag cggagcgttc gtgcgcgcag cctggctgag
     3301 ctcgctcgcg cttttgtggt tcgggtgcgc gccgccgagc gctcgtccgg agagtgaggc
     3361 ggtcgccgaa cgcgtatcac ccgcccgggt ggacctgagt cggatccggc tcgggcccgg
     3421 tgttaccgaa tctacgctcg agcttgcggg cggcggagcg cgttcggtcc tgttgtggca
     3481 gcccgaaggg gagccaaact cggtcatagt ttttctgcac ggggccgttc cggttggcgc
     3541 accgcggccc tggcgcgctc cggctcagac gcgcggcttg gtcgagtgcc tcgccgttcc
     3601 cgcgttcgcg gcgctgcggc ccatcattct gctgccgcgc agcgagagag gcgaatggtg
     3661 gtcgcgcgct gacgcggagt tcgtgctcgg cctgctggaa gccgcggtgc agcgctggcc
     3721 aaccgccgcg caccgcaaag cgatcgtcgg gtacagcaat ggcggcatcg gtacgtggta
     3781 cttcgcgcgg gcctacccaa cgtacttcga cgctgcgatc ccgatggcgt cgaacgacac
     3841 ggtggtcggt gccacgcgct taccggtctt cgcgattcac ggcgaaaatg acgaactttt
     3901 ctcgatcgaa cgggttcgct ccgcgatcga agcattgaag agcgaggggc aggacgtcac
     3961 cctgctcgcg aagtatcgcg gcacccacct ggcgccttgc ggctacgtgg acgagctcgg
     4021 caccgcagcg cgctggctct cctcgcacgt ctggcactag ggttccaagt tctccgccct
     4081 gctgcgagcc aagatccggc ggccgtgaac gtggctcgcg tgacgagtaa aaaactcggc
     4141 acaacgccga gtggtcgagc tctggttcag ccctcgggtt tgagtacgcc gagcacgtac
     4201 tccttggtgt ccaggtagcg cttcgcggac gcgcgaaccc gctcgctcgt tgcgcgctcg
     4261 agctctttcg acaaatccac gatggtgtgc ggatcgtcgc cgaaggtgta cacgcgctcg
     4321 agctcgtgca accaatatcc gttgtcgagg agctcggtct cgtgggcacg tcgacgctct
     4381 tccttcacct tcagcaggta gtcgtcggcg atgccgcgct gctggagctc cgaaattgcg
     4441 gagaagacgg cgctcgtgag cttgtccacg ttttcgggct ggcacccgaa gttgatcgac
     4501 agcgtgtact cctcacgagg ccggcgcgcg attcggccgt ttacctggac tccgtaaacg
     4561 ccgcccatgt cctcgcgcag gatctcgcgc agccggtgac gaagcacgtc ctgcaagatc
     4621 tcgaggtcgt ccgcggcgtc gcgcgaaaac ggctgcttgc catgaaaatt gagggtcacg
     4681 aggctcttgg gttcatgccc cttcacgacg accttgcgct gaattcctcg tggccaggtg
     4741 acgtgggtgt cgcgccactt ttccttacgg ccgcgcgccg gcaagcttcc aaggtacgtc
     4801 tccgcgagcg gcttgagctc gtcgagggag aagttgccga cgaacacgaa cgtgaagtcg
     4861 gaagcatccc cgaggcgatc ccgataaatg gcgaacgcct tgtccaagtc gatctgctgc
     4921 aacaggtcgg gggtgacggg acgcctgcgt gaatgatttt ggctcgtcag ggtctgcagc
     4981 tcttcgaaga acgtgagctc gggtgagagc agacgatggc tcgtgcgctc cacctcgcgc
     5041 gctcgccagg cgttgaacgt cgattcgtcg cggcgcgggg ccgtcatcgt caggtaggtg
     5101 agttgaaaca agagctcgag atctccgctc gacgcgcggc ccgaaagccc ctcgtcgagc
     5161 tcgccgatgt gcggagtcac cgaggcgagc ttgccggcca gcgctttccg gagctggatc
     5221 gcgtcgaacg cgcctagccc gcccaaggcg acgacactgt ccgcgaagcg cgccgatgcg
     5281 aaatcggcgt ctttgaccag ggagctgccg cctggtgaaa acgccgtgag gcgcacgtcg
     5341 tcgttggcga agctggtcgg cttcgcgatg acttccacgc cgttcttcaa ggtccaacgg
     5401 gtgatgtcga gatccgcgac ggtgctcgtc ttgagcacgc tgccgggcgc cggattttgc
     5461 gccatcaacg tgagatcttg attcgcttcc gagtaggcgg cgatttgcgt cgcctcgagc
     5521 tcgtggctca gcgcgagcaa tgcctccgga tggggctttt tcaagtgttc ggagccgttc
     5581 accaggatga cgcggctgcc ggcacccatc gttttgccga gctggttgag ttccgggagc
     5641 gagaacgtcg gcaagaagcg ctcgaccaaa gcgagctcgg cttcggcgcc gggcatcgtc
     5701 tcttcttgca ggaagttgcg cgaaatttcg gcggcgaagt ctcgcgagtc ggtcttgtcg
     5761 cgctccttca ccgcgtgctg gaagcgccgg agaagttggc tcttggctcg ctcgagctcg
     5821 ccttgcgtaa aaccgtgccg ctcgacgcgc agcacctcct cgagcagcgc cgaaaagcca
     5881 gtgagcgcgg cgtcatctcg gatcgcggcc gattgcctga aaacgtccgt cgaacgagtc
     5941 agcccgctcg tcgacgagtt cgccccgagg aagggcgcgt ccggttttcg gctgagctcc
     6001 tcgaaacgct cgttgagcat cgtgttgaag aggtgctcgc cgatcttacg acggtagtct
     6061 cgcgcggacg cttccggtcg gtgcggaaat tcgctcaaga tcgagacgct cgcggccggc
     6121 atttcggggt cggtctcgac actgacgaga gtgtcggggt ggctcggcag cgtcacgacg
     6181 ggccgcgggc gcgggctctt ggcaggggcg agcgtcgaaa actcggcctt gatctgcgct
     6241 tccgcgtcag acggcgcgaa gtcccccact gcgatcaccg ccatcaggtc ggggcggtac
     6301 cagtcgcgat aatagcggac gagcgtgtcg cgcgacgcat gctggatgat ctcgggtttt
     6361 ccgatggtga tgcgatcggc gtacttcgag ccagcgaaca ccaccttggc ctgcttgtcg
     6421 aagaggcgca tccccgcgcc gcgcccgagg cgccactctt cgagcacgac gccccgttct
     6481 tgctcgacct cctttggatc gaagctgatc ccgtcagccc aatcgcgcag cacgctgaac
     6541 gactgcttga agagctccgg gttgtcggtg gggacctgga gcatgtacac ggtctcgtcg
     6601 aagttcgtgt aggcgtttag gtcggcgccg aagcgcaccc cgctcttctc caagaagtcg
     6661 gtgagcgcac gctctgggaa gcgccgtgta ccgttgaagg ccatgtgctc ggtgaaatgg
     6721 gcgagcccgc gctggtcctc gtcctcgagc acggagcccg cgttgaccac cagccagacc
     6781 tgcgcgcgat gctcgggctt gtgatgcgcg agtacgtagt acgtgagccc attgtcgagg
     6841 cgccccttgg tgaggcgcgc gtcgagcggc aatgccttgt cgagctcatc gacgggagcc
     6901 gtggcgagcg ccgacgtagg cggcgcgacg accggagacg gcgccgagcg cggcgcggga
     6961 gccgcgcagc cggtgagcgc aaacaagacg aaaccaacga ccctcgagcg actcatggca
     7021 aaccacttag cgtttgccgc gagtgatgtc gagcatcagt caatgcgcgg gatgtcgtcg
     7081 acggtacgcc gcgcgcaagc gccgtatctg gcttcgaatc gcgacttcga gtcggtcgag
     7141 gcgagccgcc acccaggccg attgcaccgc gaacaatccg gcgctcagtg cgaagaacag
     7201 cactgccggc cccggaatga aagcgaacag caccccaacc gggatgagcg caatcgcgag
     7261 caccaggtag ataacgcgca cccacgccgg cctgttccgc tcttcgtcat gaaagcgttc
     7321 gaagcgctcg ccgggtggcg cttgcttcag tcgttgccac ttgtgccgga gtcgctccaa
     7381 catgttgcgt gagggatgca tcaacggtgc cgtgccagtt ttttcgccga aacgcgcgga
     7441 tttaggcgcg gcgcgcggga caagctcgcc ccgctgtgcg cccgtgctgc ggcacgaaac
     7501 tcggcatgat agggtgagtg ttggtggaag cgctgtcggg cgttgcggat cgtgggtgcc
     7561 tttacgtgtc cgcggtcctt ggactgtgcg gttgctccac gctcactcag atttcggcgg
     7621 gacccacgtt cttgccttcg tcggagcgcg actcgcgggc gggtgccgag gtgctagcgc
     7681 gagccgcgtc ggggttcggc tcggagaagg ggtttggggg gatcgaggcc gtggggcgag
     7741 cgttcgctct cggggaacag caggggttca gcgctggcct cgggcctgca tgggttcaaa
     7801 cctggggcaa gggttggctg atgttcgacg ccgacgccat gcttggtgtc gcctatacga
     7861 agcatgaacc gtgggtgctc gggacgttcc gcgccggtct cgggggcggg gtcgagctca
     7921 gttcgtcgac gtcggctcga ccgcaccggc tacttttcgg cggggaacca atgcccggcc
     7981 tggtcgagat cttccacgac gcgacagcgc tgaccatcga cctcggtcct gcagtcgatg
     8041 tcagcggagg gcagtcgccg gctttctcgc tcggtgttcg gattggcatc gcttggatcg
     8101 aactgcggta cacggaccga gatccgctcg atgcgttcac gccgtacggt cgcgggagga
     8161 ttccgttccg cgccatgcca gcgccgcgct agctcgtgcg cgtcaactct tcttgcgccg
     8221 cggcgccacc ttggcgcgcg actctggttt cgggagcggc ggtaggccca gcgcggagcg
     8281 gacgggggcg aacgagcgtc gatgaatcgg gcaggcgccg tgcttcttca gtgccgcgac
     8341 gtgttcgcgt acggggtagc ccttgtgacg cgcgaaccca taatgcgggt actcttggtc
     8401 gtattcgagc atgcgcgcgt cgcgctccgt ttttgcgatg atggacgccg cggcaatgga
     8461 caagctgcgc tcgtctccct tgacgatgcg cgtctgctcg atgggcacgt cgcgaagctt
     8521 tcgggcgtcg atgagcaggt gctccggcac caagccgagc atctcgacgg cgcgtcgcat
     8581 cgcagcgagc cccgcccagt agatattgag ccggtcgatc tcttccggct cggcgaaggc
     8641 gacgg
//
LOCUS       DASTKS010000137         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_691095, whole genome shotgun sequence.
ACCESSION   DASTKS010000137 DASTKS010000000
VERSION     DASTKS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691095"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2062)
                     /locus_tag="VFQ35_02930"
     CDS             complement(<1..>2062)
                     /locus_tag="VFQ35_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9929609.1"
                     /translation="EDRKHGLRLALKSLRRVTPSSVLQLKREFRALADVSHSNLVALH
                     ELFCEHDEWFFTMELVEGVPLTGPRGPLPLCERAGEVDALVRRFSELAQGISLLHAHG
                     ILHRDLKPGNVLLTSEGRVVILDFGLVRRLDDEPPLRHDSISFSGTLAYVAPEQVLSH
                     QLTTACDWYSFGSMLYEALVGRQPFSGRAILRDKLNGPPALPGDFSGRAKALAELCLG
                     LLAPAPERRPSGDAVLEALGAPASIAPRSGPVSGERETAIIGRDLEVAELTEAMCRTP
                     ETPTRVVLVGGASGTGKTTLVRRVLRDLSERPSTLLLSARCHPYETVPYNALDGLVDE
                     LARALSVRGVELGELVAPRDVAALFQLFPALKTAPGLLARGEPIDDPAELRRRGCSAL
                     RELLTKLGQHFELVLFIDDLHWGDADSGWLMREILLPPSAPELLFVGTFRDDERHTPL
                     VSTLLTEARLAQCRRAVSLTALSEESATRVIRGLGDFDDEVTSVILKQANGNPFLLCE
                     LSRGVSEIGAKDVPRVSLANVLSQRLGQLSPRANELMQLVVLPGRAIPVEIALDALDF
                     GSAELTDLASLRSAGLVRIRSAGATRRVEPYHDRIRETLSAELDERHKRALFARLVAA
                     LERRPNNDVELLADALQGAGQASRAATAAEKAADVAESALAFSRAADWYRTALRLGRH
                     SADAR"
BASE COUNT          383 a          693 c          691 g          295 t
ORIGIN      
        1 tgcgcgcgtc ggccgagtgc cttcccaagc ggagcgcggt tcgataccag tcggcagcgc
       61 gcgagaaggc gagcgcagac tcggcgacgt cggcggcttt ctccgcggcc gtcgccgcgc
      121 gcgatgcttg accagcgccc tggagcgcgt ctgcgaggag ctcgacgtcg ttgttgggcc
      181 ggcgctcgag cgcggccacg aggcgcgcga acaaggctcg cttgtgacgt tcgtcgagct
      241 cggcggagag cgtctcgcga atgcgatcgt ggtacggttc cacacgccgg gtcgcgccag
      301 cgctgcggat gcgaacgagc cccgccgagc gcaggctcgc gaggtcggtg agctcggcgg
      361 acccgaagtc gagcgcgtcg agggcgatct ctaccggaat ggcgcgcccg ggcaacacca
      421 cgagctgcat gagctcgttg gcgcgcggag agagctgacc caagcgctgc gagaggacgt
      481 tggctaggct caccctcggc acgtctttcg cgccgatttc ggagacgccg cgcgagagct
      541 cgcagagcag aaacggattg ccgtttgcct gcttcaaaat cacgctcgtt acttcatcgt
      601 cgaagtcgcc gaggccgcgg atcactcgcg tcgcgctctc ttcggagagc gccgtcaacg
      661 acactgcgcg ccggcattgc gcgaggcgcg cttccgtcaa cagcgtgctc acgagcggcg
      721 tatggcgctc gtcgtcacga aacgtcccca cgaacaggag ctcgggagcg ctcggcggca
      781 gcaagatttc gcgcatcagc cagccgctat ccgcatcacc ccaatgcaag tcgtcgatga
      841 acagcacgag ctcgaagtgc tggcccagct tggtcaagag ctctctcagc gccgagcagc
      901 cgcgccgtcg aagctccgcg ggatcgtcga tcggctcacc gcgcgctaac agtcccggcg
      961 ctgtcttcaa cgctggaaag agctgaaaca gggccgccac gtcgcgcgga gcgacgagct
     1021 caccgagctc tacgccgcgc acagacaagg cgcgagcgag ctcatccacg aggccatcga
     1081 gagcgttgta aggcaccgtc tcgtacgggt ggcaacgtgc cgacaagagc agcgtgctcg
     1141 gacgctccga caggtctcgg agcacccgcc tcacgagcgt cgtcttgccc gtccccgaag
     1201 cgccgccgac cagcacgacg cgcgtcggcg tttcaggggt tcggcacatc gcttcggtca
     1261 gctcggcgac ttcgaggtcc cgcccgatga tcgcggtttc gcgctcgcca gaaaccgggc
     1321 ccgagcgcgg cgcgatcgag gcgggcgcac cgagcgcttc caacaccgcg tcgcccgagg
     1381 gacggcgctc cggagccggc gcaagcagcc cgaggcacag ctcggcgagc gccttcgcac
     1441 ggccggagaa gtcccccggc agcgcgggcg gcccgttcag cttgtctcgc aagatcgcgc
     1501 gacccgaaaa cggctgacgc ccgacgagcg cctcgtagag catcgaaccg aacgaatacc
     1561 agtcgcaggc cgtcgtcagt tgatgagaga ggacttgctc tggcgcgacg taggcgagcg
     1621 tgccggaaaa gctgatgctg tcgtgacgta aaggaggctc gtcgtccaag cgccggacga
     1681 ggccgaaatc gaggataacg acgcggccct ccgaagtcag aagcacgttg cccggcttca
     1741 gatcgcggtg aaggatcccg tgtgcgtgca agagagagat cccttgagcg agctccgaaa
     1801 agcgccgcac gagcgcatcg acctcgccgg cgcgctcaca gagcggtagc ggaccgcgcg
     1861 gaccggtgag cggcaccccc tcgacgagct ccatcgtgaa gaaccactcg tcgtgttcac
     1921 aaaagagctc gtgcagtgcc accaagttcg agtggctcac gtcggccaaa gcacgaaact
     1981 cgcgcttcag ctgcagcacg gagctcggag taacccgccg caacgacttc aaggcgaggc
     2041 gaaggccgtg ctttcgatcc tc
//
LOCUS       DASTKS010000138         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_243843, whole genome shotgun sequence.
ACCESSION   DASTKS010000138 DASTKS010000000
VERSION     DASTKS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243843"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..612)
                     /locus_tag="VFQ35_02935"
     CDS             complement(4..612)
                     /locus_tag="VFQ35_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929610.1"
                     /translation="MSGQILLVEDEFDTLELLGRSLERAGHRCLLAQSLSEALGHVAK
                     HPELALVITDIVLGKDEHGGLELLKALGARSPHVPVIVTTAYADVEKLKVALNEGAAH
                     FLEKPFRASELLTAVDRALGARRTRDVDSPEQRVERVFTQAKLTEKERAVARHLLEGR
                     TSGEIAIIENNSPKTIRQHVSQIYAKCDVASRAEFFRLVYLK"
     gene            complement(609..1556)
                     /locus_tag="VFQ35_02940"
     CDS             complement(609..1556)
                     /locus_tag="VFQ35_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9929611.1"
                     /translation="MPPSRGIGGSFYVAVGSLLGAAYVAFDLWAERRLAHGTLTGALA
                     STHDLVDHLLPLLLGALLGLCVYQLRLGKRLAQAEEEAARAEALRVRLSRVERDQAVW
                     VLAASLLHEMNNPLHALGLLLDEQAACTEDPERRSELSARARSQMNRILASLSALRTL
                     RAAGEPDMQELALDRLVKSVADEVASLASARNVEVLVEGTAQVSATGDATYVRTILEN
                     LLDNSLHAVGASERGRVSIRLEVEHERAVIRVEDDGPELAQDVRAALFDPLRSTKSHG
                     LGLGLPIARALARAMRGELTLDPAARKAFRLELPMKARA"
     gene            complement(1635..2213)
                     /locus_tag="VFQ35_02945"
     CDS             complement(1635..2213)
                     /locus_tag="VFQ35_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929612.1"
                     /translation="MISSRVVTNGSPFFDVTLGGLFLEQRFGDPLVVGIQGGAYFADI
                     VRLSLRAEMPSSEATDQAGSSYDYQSSYTLQRVDADPARFIFGGTLGISVVNTQNFAF
                     SPGVLVLRSDVADYGTVLGVSLPFEWITSRGLRFGLEVDLGRAFGGTTRIGCYSAGGC
                     PPSEVGDKDREAGRALGLRFSIGYGIGFTRAR"
     gene            2332..2604
                     /locus_tag="VFQ35_02950"
     CDS             2332..2604
                     /locus_tag="VFQ35_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929613.1"
                     /translation="MVAAGAVGGVAAVVAGAGAVGATVAGAVGAGVVTAAPGAGAGGG
                     GTAGGGVGAAGAVAGARAGAGGTGKAPVCACETDASSTSDDASKAW"
     gene            complement(2814..3506)
                     /locus_tag="VFQ35_02955"
     CDS             complement(2814..3506)
                     /locus_tag="VFQ35_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9929614.1"
                     /translation="MLVGSRARAALTLSLLALASACSKTSAVEHRAAAGTASSAPARP
                     LVAQQARPKVSRATPAAATIPAAWPEASTLTAVRADQLIARIRASGKKGTLVNAWASW
                     CGPCREELPMLTSLAKNLAAQSIDVVLVSVDEPEDRPKAEAFLKSLDIKLPAYLAARP
                     LGDFKVGLNPRWPGMLPASFLFDSNGTLRYFWGGEAFENEIVTVIDGFLAGKPIDGES
                     DFALAPGKTNSP"
     gene            complement(3510..3686)
                     /locus_tag="VFQ35_02960"
     CDS             complement(3510..3686)
                     /locus_tag="VFQ35_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929615.1"
                     /translation="MPVCKGCSEEVDTLVSVKVGGKTKKLCEDCADRAREESEIAESS
                     ESVVQNMMGFKGRR"
     gene            3799..4548
                     /locus_tag="VFQ35_02965"
     CDS             3799..4548
                     /locus_tag="VFQ35_02965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9929616.1"
                     /translation="MAGRTFAIGDIHGETQHLFRLMSCLPKLDERDTLVFLGDYLDRG
                     PHSREVVEYLMELPRQTKAKVVCLRGNHEDSWLRVCKSGWDEFVLPPGNGCFAAYRSF
                     IRGPVPEDGQQPSQADLGAMCTGSFFPEAVVSWLEELLYWYEDEHAIYVHAGLPRAGD
                     RFAHPSEVADPLILLWIRTEEFFRKYRGKPVVFGHTRTEFLPQELSSYTPEDPTDLFA
                     GENAYGVDTGCGQGGFLTALEFPALRVYESR"
     gene            4663..>6003
                     /locus_tag="VFQ35_02970"
     CDS             4663..>6003
                     /locus_tag="VFQ35_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET9929617.1"
                     /translation="MSSVYPILALRGASALTPFRQARRLAELRRLVPGLRELRALYWY
                     FVELSRDLGADERSRLLAIVEAEPLVDAKGALRVIVTPRLGTISPWSSKATDILGVCG
                     LDAVRRIERATAYEFVGDLTQGDLATLATHLHDRMTESVLPSVEHAAQLFRHEAPRSL
                     TSVDVLGEGRAALVRANSELGLALAEDELDYLAQSFRELGRNPNDVELMMFAQANSEH
                     CRHKIFKATFEIDGAEQPASLFQMIQNTHAKSPNGTLSAYRDNAAVFEGSLAQRFFPS
                     PANAVYGSEREPAHILIKCETHNHPTAISPYPGASTGSGGEIRDEGATGRGAKPKVGL
                     AGFSVSNLRIPGFVQPWEEKDYGKPERIVSALSIMIEGPLGGAGFNNEFGRPAIAGYF
                     RTFECTERTGGGEVLRGYHKPIMLAGGLGNVRPVHVSKAEIPEGALLVVLGGPAL"
BASE COUNT         1001 a         1944 c         2032 g         1026 t
ORIGIN      
        1 cgcttacttc aggtacacga ggcgaaaaaa ctctgcgcgc gaagcgacgt cgcacttggc
       61 gtaaatctga ctcacgtgct ggcggatcgt cttcgggctg ttgttctcga tgatcgcgat
      121 ttcaccgctg gtgcgacctt cgagcagatg gcgagcgacc gcgcgttcct tttcggtgag
      181 cttggcttgg gtgaacactc gctcgacccg ctgctcgggg gaatcgacgt cgcgcgtgcg
      241 gcgagccccg agcgctcggt ccacggccgt gaggagctcg ctcgcgcgga agggcttctc
      301 gagaaaatgc gccgcccctt cgttgagtgc gacttttagc ttttcgacgt ccgcgtacgc
      361 cgtcgtcacg atgacgggca cgtgcggcga ccgcgcgccg agcgccttca agagctcgag
      421 ccctccgtgc tcgtctttgc cgagcacgat gtcggtgatc accagcgcga gctccggatg
      481 cttcgcgacg tggcccagcg cttcgctcaa gctctgcgcg agcagacagc gatgaccggc
      541 gcgctcgagc gagcggccga gcagctccaa ggtgtcgaat tcatcctcga cgaggagaat
      601 ctgtccactc acgcgcgcgc cttcatcgga agttcgagcc gaaacgcttt gcgcgcggcc
      661 gggtccaaag tcagctcgcc ccgcatggct cgcgccaacg cccgcgcgat gggcaggccg
      721 aggccgagcc cgtggctctt cgtcgagcgt aggggatcga agagcgcggc gcgcacgtct
      781 tgagcgagct cggggccgtc gtcctcgacg cggatgacgg cgcgttcgtg ctcgacttcg
      841 agccgaatcg agacgcgccc gcgctcgctc gcgccgactg cgtgcaggct gttgtcgagc
      901 agattctcca ggatggttcg cacgtacgtc gcgtcgcccg tcgccgagac ctgcgccgtt
      961 ccctcgacga gcacttctac gttgcgcgcc gacgcgagcg acgcgacctc gtccgcgacg
     1021 ctcttcacca aacgatccag cgcgagctcc tgcatgtcgg gctcgccggc ggcgcgcagc
     1081 gtgcgaagcg cggagagcga cgcgaggatg cgattcatct gggagcgagc gcgcgcgctg
     1141 agctccgaac gccgctcggg atcctccgtg cacgccgcct gctcgtcgag cagcaagccg
     1201 agcgcgtgca acgggttgtt catctcgtgc aacaagctcg cggccaggac ccatacggct
     1261 tggtcacgct ccacgcgcga caggcgaacg cgcagggcct ccgcgcgcgc ggcctcttct
     1321 tcggcttgcg caaggcgctt gccgagccgc agctggtaga cgcacaaccc gagcagagct
     1381 cccaaaagca acgggagcag gtgatcgacg aggtcatgcg tactcgccaa ggcgccggtc
     1441 agcgttccgt gggccaagcg gcgctctgcc cacaggtcga acgcgacgta cgccgcgccc
     1501 agcaacgacc caacggctac atagaagctt ccgccgatgc cacgtgaagg aggcatatcg
     1561 taggcattag cacgcgtccg aacgcgccgc tccgcggctc atcgaagcgt cgaggcggct
     1621 tccggtcggg atactcagcg cgctcgcgtg aaaccgatgc cgtacccgat cgagaagcgt
     1681 agcccgagag cacgccctgc ttcgcgatct ttgtcgccca cctccgaggg agggcatccg
     1741 ccggcggagt agcagccgat gcgcgtggtg ccaccgaagg cgcgccccaa atcgacttcg
     1801 agtccgaagc ggagcccgcg cgaagtgatc cattcgaacg ggagcgagac accaagcacg
     1861 gttccgtagt ccgccacgtc ggagcggaga acgaggacgc cgggtgagaa ggcgaagttt
     1921 tgcgtgttca cgacggagat ccccaaagtt cctccgaaga tgaagcgcgc cggatcggcg
     1981 tccactcgct gcaacgtata cgagctctgg tagtcgtagc tcgaccccgc ctggtcggtc
     2041 gcttcggacg acggcatctc ggcccgcagc gagaggcgga cgatgtcggc gaagtaggcg
     2101 ccaccttgga tacctacgac gagcggatcg ccgaagcgtt gttcgaggaa caggcctccg
     2161 agagtgacgt cgaagaaggg tgaaccgttc gttacgacgc gcgagctgat cacgcgagcg
     2221 tcactcggcg gagccgtcga aagatctatg tttgcgggcg ggctggacgt tggcgcgccg
     2281 ggatcgatcg gagtcgccgt tgcgggggcg actgcttcag gtgcggcggg cgtggttgct
     2341 gctggcgcgg tcgggggcgt tgcggccgtc gttgccgggg cgggagcagt gggcgcgacg
     2401 gtggcaggtg ccgtaggggc cggggtcgtg actgcggcgc cgggcgctgg agcgggaggc
     2461 ggcgggaccg ctggcggtgg cgtgggggca gccggtgcag tcgctggcgc gcgcgcagga
     2521 gcgggcggta ccggaaaggc gcccgtttgc gcctgtgaga ccgacgcgag cagcacgagc
     2581 gacgatgcga gcaaagcttg gtgaagcgaa gttaacccca tgccgatatg ctgatcgctc
     2641 gccgtcagtg ccgtcaagtg tgccccaaaa gcacacggga gaacgcctga aacgagcgcg
     2701 attcgcgctg agccgcgtct ccccgcccgc gcgcccgcct cgctgcggtc ttggctccct
     2761 cgcgaccgac tcatccgttc gcgggccttg gcagggcgtt gaaaaacgcc ctgctagggc
     2821 gagttggttt tgcctggcgc gagcgcgaag tccgactcgc cgtcgattgg cttgccggcg
     2881 aggaagccgt cgatcacggt aacgatctcg ttttcgaacg cctcgccacc ccaaaaataa
     2941 cgaagcgtgc cgttcgagtc gaagaggaag ctcgcgggga gcattcccgg ccaacgcgga
     3001 ttcagcccaa ctttgaaatc accgagcggt cgagccgcca agtaggcggg cagcttgatg
     3061 tcgaggctct tgaggaacgc ttcggctttg ggacgatctt cgggctcgtc gacggaaacg
     3121 agcaccacgt cgatcgactg ggcggcgagg ttcttggcga ggctcgtgag catgggcagc
     3181 tcttcgcggc acggcccgca ccagctcgcc caggcgttga cgagcgtacc cttcttgccg
     3241 ctcgcacgaa ttcgagcgat tagttggtcg gcgcgaacgg cggtgagcgt gctcgcctcc
     3301 ggccacgccg cagggatcgt ggcggcggca ggcgtcgcgc gcgacacttt cggacgggct
     3361 tgctgagcga cgagaggtcg cgccggggcg gagctcgcgg tgccggcggc ggctcgatgc
     3421 tcgaccgcgc tcgttttgga gcaagcgctc gccagcgcga gcagtgacag agtgagcgca
     3481 gcgcgcgctc gagaacctac gagcatgcgt cagcgccgac ctttgaatcc catcatgttt
     3541 tgaacgacgc tttcgctgct ttcggcgatc tcgctctctt cgcgagcgcg gtccgcgcag
     3601 tcttcgcaaa gcttcttggt ctttcccccg acctttaccg aaaccagagt atcgacttct
     3661 tcggagcagc ctttgcagac gggcatgggg ccgagtgtaa cctctcccgc ggcgtcaggc
     3721 tttcgttccc aatttgggcg aaaagccgag gcattcgggg ctccggggag cgcggccgcg
     3781 tgctatcgtc ggcggaaaat ggcagggcgt acgtttgcga tcggcgacat ccacggggag
     3841 acgcagcacc tgttcaggct gatgagctgc ctccccaagc tcgacgagcg cgacaccctc
     3901 gtcttcttgg gcgactactt ggaccgaggg ccgcattcgc gcgaggtggt cgagtacctg
     3961 atggagttgc ctcgccaaac caaggccaag gtcgtgtgct tgcgcggcaa ccacgaagat
     4021 tcctggttac gcgtttgtaa gagtggttgg gacgagttcg tacttccgcc cggcaacggt
     4081 tgtttcgctg cgtatcggag cttcatccgc gggccggtac ccgaggacgg acagcagccg
     4141 agccaggcgg atctcggcgc gatgtgcacg ggatccttct ttccggaggc agtggttagt
     4201 tggctcgagg agctgctcta ctggtacgag gacgaacacg cgatttacgt gcacgcgggg
     4261 ctgccgcgcg cgggtgatcg cttcgcgcac ccgtcggagg tcgccgatcc attgattttg
     4321 ctgtggatcc gcacggaaga gttcttccgg aagtaccggg gcaagccggt cgtcttcggt
     4381 cacacgcgga ccgagttttt gcctcaggag ctctcgtcgt acacgcccga agacccaacc
     4441 gacctcttcg ccggcgagaa tgcctacgga gtcgacactg gctgcggcca gggcgggttt
     4501 ttgactgcac tcgaattccc cgcgttgcgc gtctacgaat cccggtagcg gcggccgacg
     4561 ccgacgaaac gtcgcgctca cgggctccag atcggcagct tttcggcgca tccggcgcgt
     4621 cgcttgcggc gatggtcccg ctctgcaaat aagcgcctcg gagtgagcag cgtgtacccg
     4681 attttggcat tgcgcggcgc ctcggcgctg accccttttc ggcaggcacg acgcctcgcc
     4741 gagcttcgtc gactcgtccc tggcctgcgc gagctgagag cgctgtactg gtacttcgtc
     4801 gagctctcgc gcgacctcgg tgccgacgag cgctcgcgct tgctcgccat cgtcgaggcg
     4861 gagccgctcg tcgacgcgaa aggggcgctt cgcgtcatcg tcacgccccg gctcggaacg
     4921 atttcgccgt ggtcgtccaa agccaccgac atcctcggcg tgtgtggcct cgacgccgtg
     4981 cgccgaatcg agcgcgccac cgcctacgag ttcgtcggag acctcaccca aggtgatctc
     5041 gcgacgctcg cgacgcatct ccacgaccgc atgaccgaga gcgtgctgcc gagcgtcgag
     5101 cacgcggctc agctcttccg ccacgaagcg cctcgcagcc tgacgagcgt cgacgtgctc
     5161 ggcgaagggc gcgccgcgct ggtgcgcgcg aattcggagc tcgggctcgc gctcgccgaa
     5221 gacgagctcg actacttggc gcagagcttc cgtgagctcg gtcgcaaccc gaacgacgtc
     5281 gagctcatga tgttcgcgca ggcgaacagc gagcactgtc gccacaagat cttcaaggcg
     5341 actttcgaga tcgacggcgc cgaacagccc gcgtcgctgt tccagatgat tcagaacaca
     5401 cacgcgaaga gcccgaacgg cactttgtct gcgtatcgcg acaacgcggc ggtgttcgaa
     5461 ggctcgctcg cgcagcgctt cttcccgtca ccggccaatg cggtctacgg ctccgagcgg
     5521 gagccggcgc acatcctgat caagtgcgag acccacaacc acccgaccgc gatttcgccg
     5581 tatcccggag cctcgaccgg atccggcggc gagattcgag acgaaggggc gaccgggcgc
     5641 ggcgcaaagc cgaaggtggg cctcgccggc ttctcggtct cgaaccttcg tatcccgggc
     5701 ttcgtccagc cctgggaaga gaaggactac ggaaagcccg agcgcatcgt ctcggctctc
     5761 tcgatcatga tcgagggccc ccttggcgga gccggcttca acaacgaatt cgggcggcct
     5821 gccatcgccg ggtacttccg caccttcgag tgcacggagc gcaccggcgg cggcgaggtg
     5881 ctgcgcggct atcacaagcc gatcatgctc gcgggcgggc tcggcaacgt gcgccccgtt
     5941 cacgtgagca aagcggagat tccggagggc gcgttgctgg tggtgctcgg cggtcccgcg
     6001 ctc
//
LOCUS       DASTKS010000139         5365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1583723, whole genome shotgun sequence.
ACCESSION   DASTKS010000139 DASTKS010000000
VERSION     DASTKS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583723"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFQ35_02975"
     CDS             <1..1142
                     /locus_tag="VFQ35_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929618.1"
                     /translation="HHEEEDLPGFVYDRLKAAVAEPTSTKVTTKMETPILYFYSKDAL
                     DVSVRVDFPTGILTQWYPGVVDFRPQILWEQPQSVTSPPKDPAFDLSVALPTLECEDK
                     NRRNGWLDWGSFRVLGRSETAPMPPSAPKDRFTWSYARDVDANFVKFPNGESERFLFY
                     RGLSNAIPPVVVRALRADGQGQRLSLVNASRARVPSAMVLRVDENGGAFTELPTGIPA
                     TGELGVTAPEPAQFLPLGTYVDALGAAMRSTLEAQGLFGDEAAAMVNTWSKQWFTTPG
                     LRVLYFAPPEWTEATIPLRLAPAPRELVRIMVMRVEVLTPELEDRDLAALNGADDSAM
                     RSYFAALGRFAEPRLRRALSQTPSVAGNAFLGELAQSVSRAGTAE"
     gene            1179..>5365
                     /locus_tag="VFQ35_02980"
     CDS             1179..>5365
                     /locus_tag="VFQ35_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9929619.1"
                     /translation="MTVLHPTQPYVLSHPRYRSLGELGRGSQGVVVRVTDAEAPERAL
                     VAKVYLRGELDRESFRSEFRLLARLDAPGLVRVRDLCVDEATNRPFLVEDFVAGRTIE
                     EEVQSAPEAQRSQSLARLLASAAHALATLHAAGFVHGDLKPAHVKINHQGEAVLLDLG
                     SAVAQASAAPPSATAGYLAPERTPGSRATVASDLYALGAVAFKAATGDLPEPGERGLR
                     LRATWLDARVADAIEALVARGAHERPSDAADVIARVGRAVPEPGDLRRHFGTVGREQE
                     LAELASGVRRGVTYLCGPSGIGKSHLLRELWVGALLAGRAARLLAFGEVAEAELGKVI
                     AFLHGRALDLFLHSSEQRGLLLLDGLDEAPREVRAAVEAFRCREREPNVDIVVATRTA
                     PTRVAVMELGPLSEPSAAELAQSFGAAPPREREHALEMSRGNPGWLIAALGGAAVTRE
                     MAAERLQALPHGAARVVFAVALANGELSEAALRSFLLWRSGGAPLELTQALDSGLIAR
                     RATPRGTLYVVAVPAFARELVAAPMDSCRDVVEPLVRALLAQPDAGAQQLLRCAEALP
                     SSDLREAALARAREQAERCAMPSVEMAALGLLLGDAEPRDPKRLLRLERLTRDTGRQA
                     DFPSLVEWLERAVRDEPSIEPLVLRRRAETAARQGLHEQAAELVARALTRAAGAPVGE
                     ALVHATAGALFLYRADWRAAQAEFDVADALLRAVPEADGPDPEELARLEHNRGVVALY
                     AGRLTEAERRFRAAIARKRALGDLPGVRACLLNLGLTATKLKAYDEAESALDEAVALA
                     RSLSQPVGEAWSLCARCELELERKMPAAAEHWLRQGSELAGSAPLLIQTELRLLAARL
                     ALLRGDSKTALALLDELEPRLASADAAQRGRALLIRGEAALLDLPAAPRRAARLAARS
                     LRVAREAALAELEQSARDLLRRARPQRHEPKASYPESVNREDAEPEWRWVKELVRAQD
                     QASVLIELCRLAVARTNAERALVLCLDAEGALLQAWGSDLDGFALSNAEERFTDELAA
                     ALSAHGSWFYQREVRGGSRVAARAPVGADLHVAIVLEHRFRSAQFDQLSSSELERWTT
                     LAGLALRLFAPSEGVARVSVSSVEPDEATRLPLRAARRHFPDIVGVSAALEPALQRLD
                     AAIDGEQPVLIRGETGAGKELFARALHDHGVRRARPFVAINCAAIADSLFEAELFGHS
                     RGAFTGAERARPGLLAEAEGGTLLLDEIGELSLARQATLLRLLETGRYRSVGSDKEQV
                     STARIVTATNRELERAVSEGEFRRDLFFRINVLEIRIPPLRERPEDVPLLAQTFLRRA
                     NQPIEISDSAMAQLAAYSWPGNVRELAHVIERLIALGARRIEVEDLPRAIRRTALAKL
                     EAPSGRPAPRELSERDEV"
BASE COUNT          820 a         1810 c         1852 g          883 t
ORIGIN      
        1 tgcatcacga agaggaagac ctgccgggct tcgtttacga ccgactgaag gctgccgtcg
       61 cggagccgac gagcaccaaa gtcacgacca agatggagac gccgattctc tatttctatt
      121 ccaaggacgc gctcgacgtg agtgtccgcg tggactttcc aacgggcatt ttgacgcagt
      181 ggtatccggg cgtcgtcgac tttcgtcccc agatcttgtg ggagcagccc cagtcggtta
      241 cttcgccgcc caaggatccg gcgttcgacc tcagcgtcgc ccttccgacg ctcgagtgcg
      301 aagacaagaa ccgtagaaac ggctggctcg actgggggag cttccgcgtg ctcggacgct
      361 ccgaaacggc tccgatgccg ccgagcgcgc ccaaggatcg attcacctgg agctacgcac
      421 gcgacgtgga cgcgaacttc gtgaaattcc cgaacggcga gagcgagcgt ttcctctttt
      481 atcgagggct ctcgaacgca attccacccg tcgtcgtccg tgcgctgcgc gcggatgggc
      541 agggtcagcg tttgtcgctc gtcaatgcga gccgcgcccg agtgccgagc gcgatggtgc
      601 tgcgtgtgga tgagaacggc ggggctttca ccgaactgcc aactggtatt ccggccaccg
      661 gtgagctcgg cgtgaccgcc cccgaaccgg cgcagttcct ccccctcgga acttacgtgg
      721 acgcgctcgg agcggccatg cgctcgaccc tcgaagcgca ggggctgttc ggtgacgagg
      781 cggccgcgat ggtgaacacg tggagcaagc agtggttcac gactcccggg cttcgggtgc
      841 tctatttcgc tccgcccgag tggaccgaag ccacgatacc gcttcggcta gcgccagctc
      901 cgcgcgaatt ggtgcgcatc atggtaatgc gagtagaagt cctgactccg gagctcgaag
      961 atcgcgattt ggcggcgctg aacggcgccg atgacagcgc catgcggagc tacttcgctg
     1021 ccctcgggcg cttcgccgaa ccgcggttgc gtcgcgccct ttcccaaacc ccgagcgtcg
     1081 ccggtaacgc tttcctcggc gaactcgccc agagcgtgag ccgcgcgggc acagccgagt
     1141 agggagcgcg gaaaggcatt atagtcgagg gctcccgagt gacagtgttg cacccgaccc
     1201 agccttacgt gttgagccac ccgcgctatc ggtcgctcgg cgagctgggc cgcgggagcc
     1261 agggcgtcgt cgtacgcgtg accgacgcgg aagcgccgga gcgggctttg gtcgcgaaag
     1321 tctatctgcg cggcgagctc gaccgcgaaa gctttcgctc ggagtttcgg ctgctcgcac
     1381 ggctcgacgc gcccggcctc gtgcgggtgc gcgatctctg cgtcgacgag gcgacgaatc
     1441 gccccttctt ggtggaggac ttcgttgcgg gacgaacgat cgaagaggag gttcagtctg
     1501 ctcccgaggc gcagcgttcg cagtccctcg cgcgcctctt ggcgagtgcg gcccacgcgc
     1561 tcgcgacgct gcacgccgcc ggtttcgtgc acggcgatct caagcccgca cacgtgaaga
     1621 taaaccatca gggtgaagcc gtgctgctcg atctcggctc ggcggtggcg caggcgagcg
     1681 ccgcgccgcc gagtgcaacg gcaggctatc tcgctccgga gcgcaccccg ggttcacgcg
     1741 ccaccgtcgc gagcgacctt tatgcgctcg gcgcggttgc gttcaaggcg gctaccggcg
     1801 accttcccga gccgggagag cgcggtcttc gtttgcgagc gacctggctc gatgcgcgtg
     1861 ttgcagacgc gatcgaggcg ctggtcgcgc gcggagcgca cgaacgacct tcggatgcgg
     1921 ccgacgtcat cgcgcgcgtc ggccgcgcgg tgccggagcc cggcgacctg cgccgtcact
     1981 tcggaacggt agggcgcgag caggagctcg ccgagttggc gagcggcgtt cggcgcgggg
     2041 tcacgtacct gtgcggacca agcggcattg gcaaatcgca cttgctgcgg gagctgtggg
     2101 tcggcgcgct cctcgctgga cgtgccgccc gcttgttggc ctttggcgag gtggcggagg
     2161 ccgagctcgg caaggtcatc gctttcttgc atgggcgcgc gctcgacctc tttctgcatt
     2221 cgagcgagca gcgcggcctg ctcctgctcg acggcctcga cgaggcgccg agggaggttc
     2281 gggccgcggt cgaggccttt cgttgccgtg aacgcgagcc gaacgtcgac atcgtcgtcg
     2341 ccacccgcac cgcgccgacg cgcgtcgcag tgatggagct cggcccgcta tcggagccga
     2401 gcgcggcgga gctcgctcaa tcattcggcg cggcgccacc acgagagcgc gagcacgcct
     2461 tagaaatgag ccgcggcaat cctggctggc tcatcgccgc gctcggcggg gccgcggtca
     2521 cccgcgagat ggcggcggag cgcttgcagg cgcttccgca cggcgccgcg cgcgtggtgt
     2581 ttgcggttgc cctcgccaat ggagaactct ccgaagccgc gctccgctct tttttgctgt
     2641 ggcgttcggg aggcgctccg ctcgagctca cgcaagcact cgacagcggg ctcatcgcgc
     2701 gccgggcgac cccgcgcgga acgctctacg tcgttgcggt ccccgcgttc gcgcgagagc
     2761 tcgtggccgc accaatggat tcgtgtcggg acgtcgtcga gccgctcgtg cgcgcgttgc
     2821 tggctcagcc cgacgcgggc gcgcagcaat tgctccgctg cgccgaagca ttgccgtcga
     2881 gcgatttgcg cgaagcagcg ctggcgcgcg ctcgagagca ggcggagcgt tgcgcgatgc
     2941 cgagcgtcga gatggccgcg ctcggcttgt tgctcggcga tgccgagccg cgcgatccca
     3001 aacggctcct tcggctcgaa cgactgactc gcgacacggg ccgtcaagcg gacttcccga
     3061 gcttggtcga atggctggaa cgagccgtgc gcgacgagcc cagcatcgag cctctagtgc
     3121 tgcgccgccg cgcggagacc gccgcgcggc aagggttgca cgaacaagcc gcagagctcg
     3181 tcgcgcgggc cctcacgcga gcggcggggg ctccggttgg ggaggcgctc gtccacgcga
     3241 ccgcgggcgc gctgtttctg tatcgtgccg actggcgcgc tgctcaggca gagttcgacg
     3301 ttgccgatgc cctgctgcgc gcggtacctg aagccgacgg cccagatcca gaagagctcg
     3361 cgcgtctcga acacaaccgc ggcgtggtcg cgttgtacgc ggggaggttg accgaagcgg
     3421 agcgccgctt tcgagcggcc attgcccgaa agcgcgccct cggggatctg cctggcgtgc
     3481 gggcgtgtct gctgaacttg gggctgaccg cgacgaagct caaggcctac gatgaagccg
     3541 aatcggcgct cgacgaggcg gtggcgctcg ctcgctcgtt gagtcagccc gtcggtgagg
     3601 cctggtcgct ctgcgcccgc tgcgagctcg agctcgagcg aaagatgccg gccgccgccg
     3661 agcactggct ccgccaaggg agcgaactcg ccggctctgc accacttctc attcaaacgg
     3721 agctgcgact cctcgcggcg cgactcgcgc tcttgcgcgg tgactcgaaa acagcgctcg
     3781 cgctcctgga cgaactcgag cctcgcctgg cgagcgctga cgcagcccaa cgcggtcggg
     3841 cgctcctcat tcgcggcgag gcagccttgc tcgatttacc ggccgcgccg cgccgcgccg
     3901 ctcgcctagc ggcgcgaagc ctgcgcgttg cacgcgaagc cgcgctcgcc gagctcgagc
     3961 agtcggcgcg ggatttgctc cggcgcgcgc gcccccaacg acacgagccc aaagcgagct
     4021 atcctgaatc ggtgaatcgg gaggatgcgg aaccggaatg gcgatgggtg aaggagctcg
     4081 tccgcgccca agaccaagct tccgtgctga ttgagctctg ccggctcgcc gtcgctcgca
     4141 cgaacgccga acgggcgctc gtgctctgcc tcgacgctga gggagcgttg cttcaagcgt
     4201 ggggaagcga tttggacggt ttcgcgctct cgaacgcgga ggagcgcttc accgatgagc
     4261 tcgcggcggc gctctccgcg catggctctt ggttttatca gcgcgaggtc aggggcggtt
     4321 cgcgcgtcgc cgctcgggcg ccggtcgggg ccgaccttca cgtcgcgatc gtgctcgaac
     4381 accgcttccg cagcgctcag ttcgaccagc tgagctcgag tgagctcgaa cgctggacga
     4441 cgctcgccgg gctcgcacta cggcttttcg cgccgagcga aggcgtggcg cgggtctccg
     4501 tcagcagcgt cgaacccgac gaggcgaccc gcttgccgct ccgtgccgca cggcgccatt
     4561 tcccggacat cgtcggcgtg agcgcggcgc tcgagcccgc gttgcagcgc ctcgacgcgg
     4621 cgatcgacgg agagcagccg gtgctcatac gcggcgaaac gggcgccgga aaagagctct
     4681 tcgcgcgcgc gctgcacgac cacggcgtcc gacgcgcgcg gccgttcgtg gcgatcaact
     4741 gcgccgcaat cgccgacagc ttgttcgagg ccgagctctt cggccacagc cgcggggcgt
     4801 ttaccggtgc cgagcgggca cgccccgggc tgctcgccga agccgaaggc ggcaccctct
     4861 tgctcgatga gataggcgag ctctccttgg cgcgacaagc cacgctgctc cgtctgctcg
     4921 agacgggtcg ctatcgctcg gttggctccg acaaggagca agtcagcacc gcgcgcatcg
     4981 tcacggccac gaaccgcgag ctcgaacgag ccgtgagcga gggggagttc cggcgcgatc
     5041 tcttctttcg catcaacgtg ctcgaaatcc gcattcctcc cttgcgcgag cgcccggagg
     5101 acgtgccgct cctcgcgcaa acgttcttgc ggcgcgcaaa ccaacccatc gaaatcagcg
     5161 attccgccat ggctcagctc gcggcgtact cgtggcccgg taacgtccga gagctagccc
     5221 acgtgatcga gcgactcatc gcgctcggcg cgcgacgcat cgaggtcgaa gatctgccgc
     5281 gagccattcg gcgcacggct ttggctaaac tcgaagctcc gagcgggcgt cccgcgccga
     5341 gagagctttc ggagcgagac gaagt
//
LOCUS       DASTKS010000140         7012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_914082, whole genome shotgun sequence.
ACCESSION   DASTKS010000140 DASTKS010000000
VERSION     DASTKS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914082"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="VFQ35_02985"
     CDS             complement(<1..1612)
                     /locus_tag="VFQ35_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929620.1"
                     /translation="MMRRPSLGSAAVVALALALHAGSAFALDSGAARSQAETAIQHIE
                     TEVVRGTATGGRITVPQATPAERVAAGDMLLRTKDYDRAVAELSKVLELHRQGKVPEA
                     AYADATFMIGEAYFQSRQYLSARRHFREIVDQGNKGIFEGYAGRAVSRLVDVALRTGD
                     IESLDYVFARLDRLPASDRSGSLAYARAKALFAKKDFAAARQAVNLVPAGSDYALQSQ
                     YLLGVVLVREAAPVIPPSAPTVATPGAVAKPTPGQPPRFAAAIEQFRKVTRMPAPTDA
                     QKHVVDLAWMAIGRLFHENDAYLDAAEAYSHIDRASPEFATMLYELAWVYVRLGDFMR
                     AQRALEVLAILDPRNIEAADGSLLRADLMLRSGQFDKALALYKSVQGKFDPVREQVDR
                     FLQSTNDPAVYYDKLTADQTISGDEKLPAIVIDWAREQAEDEHVFGMIEDVNRSRELI
                     RNGRKLAARLNAVLNGPTRVRALPEIQAKLQQALTLLNRTSKARRTLAEGLDDVSPDA
                     SGELASVRRERRDLMKRMGWLPVADVDFA"
     gene            complement(1609..2226)
                     /locus_tag="VFQ35_02990"
     CDS             complement(1609..2226)
                     /locus_tag="VFQ35_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929621.1"
                     /translation="MRDLEQRIDELKEQIRRSHTRLSLLSDTILSGGVGGARAEIAFK
                     NDISNAFRLTGAVFVLDGVVQYNKQDDSESGLLASQKEIPIFSGSIPPGDHSLQVVLH
                     LHGYGYGVFSYLRSYKFDVRSSHTFTVTEGKTVSLDVIAWEKGDVTTPLEQRPAIRFT
                     ENMKTGGGPAAPAAATPAAAPAAPASPALPSGSVSGGVSVGTGAK"
     gene            2261..2770
                     /locus_tag="VFQ35_02995"
     CDS             2261..2770
                     /locus_tag="VFQ35_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929622.1"
                     /translation="MAPAPAELPADVLEGVVVVVVVVVPEGPAVAVLVAVVVAALPAA
                     VALAPVDLVVSALVGVEALAPTVAFASVAFAPELLPAAGAAGAAGFFASCASSNGVEE
                     KHAAKAATVQALLKRIMTLSNGETGGPSCTVLLASRQKQLSRVSATSSERQHLPLKHV
                     ARQHESFGF"
     gene            2995..3618
                     /locus_tag="VFQ35_03000"
     CDS             2995..3618
                     /locus_tag="VFQ35_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929623.1"
                     /translation="MPAVQGDATDEMLMVRYQRGEAPAFAELVRRHSRAVYNFVLRQL
                     RDANLSEDVTQEVFLRVVQRASEFKHEARFSTWLYSITRNLCVDHLRRLSHRKHASLD
                     APRGGAADAEASPLVEQIADPHPRASAERGAQASQMQTSLLEALHALPDDQREVFLLR
                     EIANLPFKEIAEITDTPENTVKSRMRYALDRLRETLREFEEYARALR"
     gene            3636..4694
                     /locus_tag="VFQ35_03005"
     CDS             3636..4694
                     /locus_tag="VFQ35_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929624.1"
                     /translation="MDCEKFDRIVLDLLYDELDEITSAAGRRHVEHCTRCRDILNRMR
                     ATREVGALPLVEVPEGLELRILEAEHRARRELPTRQRLGRVVSMLAGYAMRPQLAMAA
                     LLLLVIGSSLLLLRTRPGEQKNVLVTERGVPEGEPEAVAVVPRASAERPKAKAEAKPE
                     EPKDERRAEARPSERAASAPRADSREDAQPASAPLGQSGADRGYEDALSAYRDRKYDE
                     AQRRFSEVAARGGENAASAALYAAQSARARSGCPTAAPLFDEVFRKYGDSQPGREAAW
                     QAASCYRMLGDFERARQDYEALISTPGYADRAQAALASLGDAEPSEVAAKRAAAASSS
                     APHRARPPAAAVRRTDSP"
     gene            complement(4748..5140)
                     /locus_tag="VFQ35_03010"
     CDS             complement(4748..5140)
                     /locus_tag="VFQ35_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9929625.1"
                     /translation="MSSDDPKASERPPSSNRRNAERIDVTWSVDCETEDTFLYANITN
                     ISEFGIFVMTHEPLEVGTQLTLKFAPPGSTEPFVMEGQVQWVNPVRMLADNPNPGMGI
                     RFINLTTEARERLVDMVHTIAYIRSASN"
     gene            complement(5229..>7012)
                     /locus_tag="VFQ35_03015"
     CDS             complement(5229..>7012)
                     /locus_tag="VFQ35_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929626.1"
                     /translation="GSTLVTGAGLSIAGALVLARTPVDVFSFWLGATTAAVRPQPTFD
                     VVLRDIGHAFFPWSALALPAFARLAQARDDGSLARVGLRLTLLTTALTAGVAQTALAP
                     ALGLQSFAAPAALAGACALALDELDEPGGVGRWLGLAVAASLTLLLLDFKNFPEKGLV
                     AFGVNDAVFPAAYAEDGFVAFALVALPLALSFVVLAGERRPEPRRGAFVRRSYFQWFR
                     VLRRVWSGTLWFCVLALGSACLGLDALIVSSRAFSSSLFASMSQVARALVRAGWLTSL
                     GIVFGPPLVKLVQDGIALLLASRVAQRFRSAPRGVVAALAFSAGGVGLSFGYYTALAA
                     ELSPKRAFDEYERLGASAPLGLLGVSAAVARYEAGVTGRSLVDADEAAAFLLADVSRR
                     FIALRANELAGLNAVFRSKVTPRRNLPVLDSHAGEIVLASNRLDASRSSDNPFDRWVT
                     SSPPAPAHPLDVDLGGRLTVLGWDVFDARGARVSALSPSVTYEFAIYYRVDQRVAANW
                     DTFLHIDGFQRRFNGDHPTLGGHYPFALWLPGDFVVDRHEFHLGPEFSRGRYDVYFGL
                     YSGSRRLEVRRGAHQDDRIIAGKLDVK"
BASE COUNT         1288 a         2297 c         2338 g         1089 t
ORIGIN      
        1 gtgcaaaatc gacgtccgcg acgggcaacc agcccatgcg cttcatcagg tcccgccgct
       61 cgcgccgtac gctcgcgagc tcacccgatg cgtctggcga aacgtcgtcc aacccttcgg
      121 caagcgtgcg gcgcgctttg gaggtgcggt tcaacagcgt gagcgcctgc tgcagcttgg
      181 cctggatctc cggcagggcc cgaactcgag tcggcccgtt gagcacggcg ttcaagcgag
      241 cggcgagctt gcgcccgttg cggatgagct cgcgcgagcg gttgacgtct tcgatcatgc
      301 cgaacacgtg ctcgtcttcg gcttgctcgc gcgcccagtc gatgacgatg gcggggagct
      361 tctcgtcgcc ggagatggtc tgatcggcgg tgagcttgtc gtagtaaacg gcggggtcgt
      421 tggtcgattg gaggaagcga tcgacctgct cgcgcactgg gtcgaacttg ccctggaccg
      481 acttgtagag agcgagcgcc ttgtcgaact gaccagagcg cagcatcaag tccgcgcgca
      541 gcagcgagcc atcggcggct tcgatgttgc gtgggtcgag gatggccaag acctcgaggg
      601 cgcgctgcgc gcgcatgaag tcgccgaggc gcacgtagac ccaagcgagc tcgtagagca
      661 tcgtcgcgaa ctcgggcgaa gcgcggtcga tgtggctgta agcctctgcc gcgtcgaggt
      721 acgcgtcgtt ctcgtgaaac aggcgaccaa tcgccatcca ggcgaggtcg acgacgtgct
      781 tttgcgcgtc ggtcggggcc ggcatccgcg tcaccttccg gaactgctcg atggcagcag
      841 cgaaccgcgg cggctggcct ggagtcggtt tggcgaccgc acccggtgtg gcgaccgtcg
      901 gcgcgctcgg cggaatgacg ggagcggcct cgcgaacgag gacgacgccg agcaggtatt
      961 ggctctggag agcgtagtcg gagcctgctg ggaccagatt gactgcttga cgcgctgcgg
     1021 cgaaatcctt cttggcgaac agcgccttgg cgcgagcgta ggcgagcgag ccggagcggt
     1081 cggaagcagg aaggcgatcg agccgagcga agacgtagtc gaggctctcg atgtcgcccg
     1141 tgcgaagcgc cacgtcgacg aggcggctca ccgctcgccc agcgtaacct tcgaagatcc
     1201 ccttgttgcc ctgatcgacg atctcccgga aatgccgtcg cgctgacaga tactgacgtg
     1261 actggaagta cgcctcgccg atcatgaacg tggcgtccgc gtacgcagcc tcgggcactt
     1321 tgccttgacg gtggagctcg agcaccttcg agagctccgc gacggcacgg tcgtagtcct
     1381 tcgtgcggag gagcatgtcg ccggccgcga cgcgctcggc tggggtcgct tgcggcacgg
     1441 tgatgcgacc acccgtcgcg gtgccacgca cgacctccgt ttcgatgtgc tggatcgccg
     1501 tttcggcttg gctccgagcc gcgccggagt cgagcgcaaa cgccgacccg gcgtgcagag
     1561 ccagggcgag cgccacgacg gcagcgctgc ccaaacttgg tcgtctcatc atttggctcc
     1621 ggttcccacc gacacgccac cgctgacgct acccgatggc aacgcgggtg acgcaggtgc
     1681 ggccggtgca gccgccggag tcgctgccgc aggcgccgct gggccgccac ccgtcttcat
     1741 gttctcggta aagcgaatcg ccggacgctg ctcgagcggc gtcgtgacgt cgcctttctc
     1801 ccaagcgatg acgtcgaggc tcaccgtctt gccttccgtg acggtgaacg tatggctaga
     1861 gcgaacgtcg aacttgtagc tccggaggta ggagaagacg ccgtaaccgt agccgtgaag
     1921 atgcagcacc acttgcagtg agtggtcgcc gggagggatg gaccctgaga aaatcgggat
     1981 ctctttctga gaagcgagca accccgactc cgagtcgtct tgcttgttgt actggacgac
     2041 gccgtcgagc acgaaaaccg cgccggtgag gcggaaagcg ttgctgatgt cgttcttgaa
     2101 cgcgatttcg gcgcgcgcgc cccccacgcc gccggagagg atcgtgtcgc tcagcagact
     2161 gagccgcgtg tgactgcgtc ggatctgctc cttcagctcg tcgatacgct gctcgaggtc
     2221 gcgcaacctg acgacgtagg tcgacccgtc gagggtaccc gtggcacccg cccccgcgga
     2281 gctcccggcc gacgtgctcg agggcgtcgt cgtggtggtc gtggtcgtgg ttccagaggg
     2341 ccccgccgtc gccgtgctgg tcgctgtcgt cgtcgcggca cttccagcag ccgttgcgct
     2401 ggcgccagtc gacttggtcg tgtcggcctt ggtaggggtc gaggcgcttg cacctacggt
     2461 agctttcgca tccgtggcct tcgccccaga gctgctgcct gcggcgggcg cggccggcgc
     2521 agcgggcttc ttcgcgtcct gcgcaagcag caacggggtc gaggagaaac acgccgcgaa
     2581 agcggcaacc gtccaagctc tattgaagcg catcatgacc ctctctaatg gggagaccgg
     2641 gggaccgagt tgcacagtcc tgcttgcgtc acgtcaaaaa cagctgtcgc gagtgagcgc
     2701 aacctcctcc gaacggcagc atcttcccct caagcacgtc gctcgccaac atgaaagttt
     2761 cggcttctag tccctcatga aagaccggcc aatcgaccca catctcggcc tcgtggccat
     2821 tgtcaaaacg tagccatctt gcgggcttcg gggtgacggc gtaccgtcca aagcacgcga
     2881 gcggagtgga gatcgatctc cgttgccgaa cgtcagaaac ccatcggcag cgcgagctcg
     2941 ggatccgtgg tcacgcatct tttcccctcc cccgccgcgc actggtcacc ccctatgccg
     3001 gccgtgcagg gcgatgcaac ggacgaaatg ctcatggtcc gctatcagcg gggcgaagct
     3061 ccagcatttg ctgagctcgt ccgacgccat agccgcgccg tatacaattt cgttctacgc
     3121 caactccgag acgcgaacct ttccgaagac gtcacgcaag aggtgttctt gcgagtcgtg
     3181 cagcgcgcga gcgaattcaa gcacgaagcg cggttttcga cgtggctcta cagcatcacg
     3241 cggaacttgt gcgtcgatca cctgaggcga ctctcgcatc gcaagcacgc ttcgctcgac
     3301 gcgccccgtg gcggcgccgc cgatgccgaa gcgtcgccgt tggtcgagca gatcgccgac
     3361 ccgcatccgc gcgcgagcgc cgagcgcggc gcccaagcgt cgcaaatgca aacctcgctg
     3421 ctcgaagcgc tgcacgcgct ccccgatgat caacgcgagg tgttcctctt gcgggagatc
     3481 gcgaacctcc ctttcaagga gatcgccgag atcaccgaca cgccggaaaa caccgtaaaa
     3541 agccgcatgc gctacgcgct cgaccgcttg cgcgagacgc tgcgggaatt cgaagaatac
     3601 gcgcgcgcgc tgcgctgact tgacgccgag gccccgtgga ttgcgagaag ttcgaccgaa
     3661 tcgtgctgga cctgctctac gacgagctcg acgaaatcac gagcgcggcc gggcgtcgtc
     3721 atgtggagca ttgcacgcgc tgccgcgaca tcctgaatcg catgcgcgcg acgcgcgaag
     3781 tcggtgcgct gccgctcgtc gaagtgccgg aaggcctcga gcttcgcatc ctggaggcgg
     3841 agcaccgcgc gcgtcgggag ctgccgacga ggcaacggct cggccgcgtg gtgagcatgc
     3901 tagccggcta cgcaatgcgc ccgcagctcg ccatggccgc gctcttgctg ctggtcatcg
     3961 gctcgagcct gctccttctg cgcacgcgcc caggcgaaca gaagaacgtg ctcgtcaccg
     4021 agcgcggcgt gccggagggt gagcccgagg cggtcgccgt cgttccgcgc gcttctgccg
     4081 agcgtccgaa agcgaaggcc gaagcgaagc cggaagagcc gaaggacgag cgtcgggccg
     4141 aggcgcgacc gagcgagcgc gccgcgagcg cgccccgcgc cgattcgcga gaggacgcac
     4201 aacccgcgag cgcgccgctc ggacagagcg gcgccgatcg cggctacgag gacgcgctca
     4261 gcgcctaccg cgatcgcaaa tacgacgagg cgcagcgtcg cttttcggag gtcgcggcgc
     4321 gcggaggaga gaacgcagct tcggccgcac tctacgccgc tcagtcggcg cgcgcccgct
     4381 cgggctgtcc tacggccgcg cctctattcg acgaggtctt ccgcaagtac ggcgactcgc
     4441 agccgggccg cgaagcggcg tggcaagcag cgagctgcta ccgcatgctc ggcgacttcg
     4501 agcgcgctcg gcaagactac gaagcgctca tcagcacacc gggctacgcc gatcgtgctc
     4561 aagcggcgct cgcatcgctg ggcgacgcgg agccgagtga agtcgcagca aagcgcgctg
     4621 ccgccgcgag ttcgagcgcg ccgcatcgcg cacgccctcc cgccgccgcg gttcgccgca
     4681 ccgactcgcc ctgagtctcg cggctcagag tcaggagcca agcgaactcc gccgagcaaa
     4741 cgctctctca gttcgacgcg ctacggatgt acgcgatggt atggaccatg tcgacgagcc
     4801 gctcgcgcgc ttctgtcgtc aggttgatga agcgaatgcc catgcccgga ttggggttgt
     4861 ccgcgagcat gcgaaccggg ttcacccact gaacctgacc ctccatcacg aacggctccg
     4921 tgctgccggg cggcgcgaac ttcagcgtga gctgcgtgcc cacctcgagc ggctcgtgtg
     4981 tcatcacgaa gatcccgaac tcggagatgt tcgtgatgtt ggcgtagagg aaagtgtcct
     5041 ccgtttcgca gtcgaccgac caggttacgt cgatgcgctc ggcatttcga cgattggagc
     5101 tcggcgggcg ctcggaagct ttggggtcgt cgctgctcac cagaactcac ctagcaccgc
     5161 tctcgggcgg atgggtagca gaggacgcag cgcactcgca tgtttgcagg ataaggtcgc
     5221 actcgcgact atttcacgtc tagtttccct gcgatgatgc ggtcgtcttg atgagcgccg
     5281 cgccggacct caagccggcg cgagcccgag tacagcccga aatacacatc gtagcgtcca
     5341 cgagagaact cgggaccgag gtgaaactca tgccgatcga cgacgaagtc tcccggcaac
     5401 cacaacgcaa acgggtagtg accgccgagc gtaggatgat cgccgttgaa gcgccgctgg
     5461 aaaccatcga tgtgcaagaa ggtgtcccaa tttgcggcga cgcgctgatc gactcgatag
     5521 tagatcgcaa actcgtaagt caccgagggc gacaatgcgc tgacgcgcgc gccgcgtgca
     5581 tcgaacacgt cccaaccgag cacggtgagg cgcccaccca agtcgacgtc gagtggatga
     5641 gcgggcgcag gcggagagct cgtgacccat cgatcgaacg ggttgtcgct gcttcgcgaa
     5701 gcgtcgagcc gattgctcgc gagcacgatt tcgcccgcgt gcgagtccag caccgggagg
     5761 ttacgccgcg gcgtgacctt cgagcggaat acggcgttca agcccgcgag ttcgttcgcg
     5821 cggagggcga tgaagcgacg agagacatcc gccaaaagaa aggcggccgc ctcgtcggcg
     5881 tcgacgagag agcggccggt gacgccagct tcgtatcggg ccacggccgc gctcacgccg
     5941 agcaagccga gaggagccga tgcgccgagg cgctcgtatt cgtcgaaagc gcgcttcggc
     6001 gagagctcgg ccgcgagcgc ggtgtagtaa ccgaaagata gaccaacgcc tccagcgctg
     6061 aacgcgagcg cggcaacgac gccgcgcggc gcgcttcgaa atcgctgggc tacacggctt
     6121 gcgaggagca gcgcgatgcc atcctgcacg agcttcacga gcggcggtcc gaacacgatg
     6181 ccgagcgacg tcagccatcc tgcccgcacg agggcgcgcg cgacttgcga catcgacgcg
     6241 aacagcgacg acgagaacgc gcgagacgaa acgatgagcg catcgagccc gagacacgcg
     6301 ctaccgagcg cgagcacgca gaaccacaag gtgccggacc acacccggcg gagcacgcga
     6361 aaccactgga agtagctgcg ccgcacgaac gctccacgcc gcggttcggg ccttcgctcc
     6421 ccggcgagca cgacgaacga aagcgccaac ggcagcgcga cgagagcaaa ggcgacgaaa
     6481 ccgtcctcgg cgtaagcggc tgggaatacg gcgtcgttca cgccgaaggc gacgagcccc
     6541 ttctccggga aattcttgaa atcgagcaac agcaacgtga gcgacgcggc gacggcaagg
     6601 ccgagccaac gcccgacgcc acccggttcg tcgagttcgt cgagcgcgag tgcgcaagcg
     6661 ccggcgagcg ccgcaggcgc tgcgaacgac tggagaccga gagcgggcgc gagagccgtc
     6721 tgcgcgacac ccgctgtcaa cgccgtcgtg aggagggtga gccgcaaacc gacccgcgca
     6781 agcgagccat cgtctcgcgc ctgagcgagc cgggcgaaag ctggcagcgc aagcgcactc
     6841 cagggaaaga aggcgtgacc gatgtccctc aacacgacgt cgaaagtcgg ttgcggtcgc
     6901 accgcagcgg tcgtcgcacc gagccaaaat gaaaacacgt cgactggagt ccgcgcgagc
     6961 acgagcgcgc ctgcgatcga gagcccggcg cccgtcacga gcgtactgcc cc
//
LOCUS       DASTKS010000141         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1806992, whole genome shotgun sequence.
ACCESSION   DASTKS010000141 DASTKS010000000
VERSION     DASTKS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806992"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFQ35_03020"
     CDS             complement(<1..292)
                     /locus_tag="VFQ35_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929627.1"
                     /translation="MSVPTLLAATARAESPSAHDPRFDVIEQLLESADFEGAEAASIR
                     LLASGALSRSEVARAHLELGIVDGARGRMKEAELEFLRAIALDATAALPATVG"
     gene            473..2848
                     /locus_tag="VFQ35_03025"
     CDS             473..2848
                     /locus_tag="VFQ35_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HET9929628.1"
                     /translation="MTVEMELHAATISARLGLERWDVLRARKDAERARALASTLGDSN
                     AVRLSDALLARTRLLCSSDPRQIELASMPAAEALASDPGLHAAVWRLKAELAAATTDP
                     NFRVEPITPQQLSLLDAAAAFAIGGVLALQSERHVPRIYGPTPALDTLSLEGWQHVLI
                     ALSLETSGGSGWSAIEQALTFALAQDRKALAWTALRLRLVMSEQRTLRAEARVTRERL
                     ARLCDEWMLSLPKAEALSARMRADRLELTERDADDGKPGLDLGSLADTAIALAHERDI
                     DTLVNLTLDAAIAATHAERGLLILRSEDGSYRVGATRYIDHVDDERATWGLSSTIVRA
                     ALDRAEAVVSNDVRRDPRFADSASAANSITSVLCVPIHARAEIEGALYLDRRVGRRPF
                     ERASISAARALGSLLAGSLLNSRVLAALESRAKELEAARAELVHALASRTAERDDMNR
                     QLAKGAIAPRGSSALIGSSPTMKRLRQHIDTVAVSDAPILIAGETGSGKELVARAVHA
                     ASPRRDRPFVAINCGALSESLLAAELFGSSRGAYTGSTTARPGLLVTADRGTVFLDEV
                     GDMPPSMQTVLLRVLETSEVRPVGSHESRKIDVRILAASHRDLLELVRQGKFRDDLRY
                     RLEVVRIEVPPLRARLDDLPELCAALLEDVRARYSLPDRQLSSAALHALRTRQWAGNV
                     RELRHALASAALASRGPLIEPADLPAERIASNLPEVEVAAESEEVDGHALRAESIRRA
                     LKATAGHRGRAAKLLGMSRSSLYRYCETYGIEIDSVAQSSSALIGRKLSAT"
     gene            complement(2893..>3206)
                     /locus_tag="VFQ35_03030"
     CDS             complement(2893..>3206)
                     /locus_tag="VFQ35_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929629.1"
                     /translation="LQHRMFANGAWYDWFNHGVKVYPETSPAVTASQPDDLDIYIKGQ
                     DGHLWGRFWVGYWTSFVDLGGILVGGPAAFQETNGRVHVIAPIDNGRHDGIWYRHWQP
                     W"
BASE COUNT          520 a         1057 c         1099 g          530 t
ORIGIN      
        1 gccctaccgt cgcggggagg gcggccgtcg cgtcgagcgc gatcgcgcga aggaattcga
       61 gctccgcttc tttcatccgc ccgcgcgcgc catcgacgat cccgagctcg aggtgcgctc
      121 gcgcgacttc gctgcgtgac aacgcaccag acgcgagaag gcgaatgctt gccgcctctg
      181 cgccttcgaa gtctgcgctc tcgagcagct gctcgatgac gtcgaaccgc gggtcatgtg
      241 ccgacgggga ctcggcgcgc gcggtggccg cgagcaaggt gggcacgctc atgagcgagc
      301 agagcgccaa ggtcgcgcgc gccggacgag cgagcgcgct ccgtgtgcgg gcggcagagc
      361 gagtccgatg acaacactct tcgattgtgc ccggacgccc gcgacgaggt cctagtaaag
      421 agggaagtat cgagtagtac cgcacggtga acgttgctaa catctaccgg agatgactgt
      481 cgagatggag ctccacgcgg caacgatcag tgcgcgactc ggtctcgaac gctgggacgt
      541 gctgcgagcg cggaaggacg ctgaacgcgc acgcgcgttg gcgagcacgc tcggcgactc
      601 gaacgcggtg cggctgagcg acgcgctgct cgcgcgcacg cgactccttt gcagttcgga
      661 tccgcggcag atcgaactcg cgtcgatgcc cgcggcggag gcactggcgt cggacccagg
      721 gctccacgcg gccgtttggc ggttgaaggc agagctcgcc gcggccacca cggatcccaa
      781 ctttcgcgtc gagccgatca cgccgcaaca gctctcgctg ctcgacgcgg cagccgcgtt
      841 cgcgattggc ggcgtcctcg ctttgcagag cgaacggcac gtcccgagga tctacgggcc
      901 aacacccgcg ctcgatacgt tgtccctcga aggctggcag cacgtcttga tagcgctttc
      961 gctcgagacc agcggcggca gcggctggag tgccatcgag caagcgctga ctttcgcgct
     1021 ggcgcaagat cggaaggctc tcgcctggac cgcgctgcgt ctccggttgg tcatgagcga
     1081 gcagcgaacg ctgcgcgcag aggcgcgcgt cacgcgggaa cggctggcgc ggctctgcga
     1141 cgagtggatg ctgagcttac ccaaggcgga ggctctttcc gcgcggatgc gggcggatcg
     1201 gctcgagctt acggagcgcg acgccgacga cggtaagccc gggctcgatc tcggctcact
     1261 cgcggatacg gcgattgcgc tagcccacga gcgcgacatc gacacgctcg tgaacttgac
     1321 gctcgacgcg gccatcgcgg cgacgcacgc ggagcgcggg cttttgatct tgcggagtga
     1381 ggacggaagc tatcgcgttg gagccacccg ctacatcgac cacgtcgacg atgagcgagc
     1441 cacttggggc ctcagctcga ccatcgtgcg tgcggcgctc gaccgcgccg aggcggtagt
     1501 atcgaacgac gttcgccgtg atccgcgctt cgccgacagc gccagcgccg cgaactctat
     1561 aacgagtgtc ttgtgcgtgc cgattcacgc gcgcgccgag atcgagggcg cgctctatct
     1621 cgatcgacgc gtcgggcggc ggcccttcga acgcgcgagc atctcggcgg cgcgcgcgct
     1681 cgggtcgctc ttggcggggt ctctcctgaa ctcacgggtc ctcgccgcgc tcgagtctcg
     1741 cgccaaagaa ctcgaggccg cgcgcgccga gctcgtgcac gcgctcgcgt ctcgaaccgc
     1801 cgagcgtgac gacatgaacc ggcagctcgc caagggcgcc atcgcgccac gtggttcgtc
     1861 ggcactcatc ggtagctccc ccacgatgaa gcggctgcgt caacacatcg ataccgtcgc
     1921 cgtttcggac gcgccgatat tgatcgcggg agagaccggt tccggcaagg agctcgtcgc
     1981 gcgcgccgtt cacgcggcga gcccgcgacg tgatcgccct ttcgtcgcca tcaattgtgg
     2041 ggcgctgagc gagtccctgc tcgccgccga gctgttcggt tcgtcgcgcg gtgcctatac
     2101 gggttcgacc accgcgcgac cgggcctttt ggtcactgct gatcgaggca cggtgttctt
     2161 ggacgaggtc ggggacatgc cgccgagcat gcaaacggtg ctgctgcgcg tactcgagac
     2221 gtcggaggta cggccggttg gctcccacga aagccggaag atcgacgtgc gcatcttggc
     2281 tgctagtcac cgcgatctct tggagctcgt gcgccaaggc aagttcaggg acgacctgcg
     2341 ctaccggctc gaggtcgtgc gcatcgaggt tccaccgttg cgtgcccgcc tcgacgactt
     2401 gccggagctc tgcgcagctc tgctcgagga cgtgcgcgcc cgttatagcc tgcccgaccg
     2461 ccagctgagc tcggccgcgc tgcacgcgtt gcggactcgt cagtgggctg gcaacgtccg
     2521 cgagttgcgg catgcgctcg ccagcgcggc gctcgcgagc cgcggcccgc tcatcgagcc
     2581 ggcggatctg ccggcggagc gcatcgcgtc gaatctgccc gaagtcgagg tcgcagcaga
     2641 gtcggaagag gtggacggcc acgctctccg agccgaatcg attcgacggg cgctgaaggc
     2701 gaccgcgggc catcggggtc gcgcggccaa gctactcggc atgtctcgct cgagcttgta
     2761 ccgctactgc gagacgtatg gcatcgagat cgacagcgtc gctcaaagct cgtccgcgct
     2821 gatcgggcgc aagctttcag cgacgtagtg gtggcgcgcc atccggcgcc gattggctgg
     2881 caggctcgcc ggtcaccatg gctgccagtg ccggtaccaa atgccgtcgt gtcgtccgtt
     2941 gtcgatgggg gcgatgacgt ggacgcgccc gttggtctct tggaaggcgg caggaccacc
     3001 gacgaggatc cctcccaagt cgacgaacga cgtccaataa ccgacccaaa acctgcccca
     3061 gaggtgaccg tcttggccct tgatgtagat atcgagatcg tcgggctgcg aggcggtcac
     3121 ggctgggctc gtctccgggt aaaccttgac cccgtgattg aaccagtcgt accaagcccc
     3181 gttagcgaac atgcggtgtt gcagaa
//
LOCUS       DASTKS010000142         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_914358, whole genome shotgun sequence.
ACCESSION   DASTKS010000142 DASTKS010000000
VERSION     DASTKS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914358"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFQ35_03035"
     CDS             complement(<1..1334)
                     /locus_tag="VFQ35_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9929630.1"
                     /translation="MLARGGHGIVFVAEHLPTERLVALKVLWPHIPESDVAVAWLALE
                     AKVAGRVKSEFIVQVLDAGVDAATYTPFLAMELLEGRSLLRVVREEGALTASQALGLL
                     SQVAQGLDHAHGFIDKAGRATPIIHRDLKPENLFLTTRDNGEPLVKILDFGVAKVLDG
                     SVTATGSIRGTPAFMAFEQISGGAVSRETDVWAFGLVAFFLLTGQNYWLAAARPELGL
                     TALCAEVLHQPLVVPSQRARALGLPSPWPSDFDAWFLRCVARDPKARFATAGSAFSAL
                     TAAFAGPLSGTARLVLGDARPAEAVMVPRREASSARRLRGEVTALARGNAATLGANLF
                     GDGVTFALFSQHASGVELCLFDAEEPSRESRRLPVSDCSNHVWRIHVPNVGAGQLYGY
                     RVHGPWDPERGHRFNPNKLLVDPYARRLVGELTWHPSVFGYDSGDPREDLSFS"
     gene            1948..>2159
                     /locus_tag="VFQ35_03040"
     CDS             1948..>2159
                     /locus_tag="VFQ35_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929631.1"
                     /translation="MPNNLTSVATAKTIKTALTPSQRSLSTTAASAANTPFAFPRFDE
                     FARRNPFVGAKVGAPQALATGNWAQE"
BASE COUNT          400 a          732 c          687 g          340 t
ORIGIN      
        1 gagaagctca gatcctcgcg cgggtcgccg gaatcgtacc cgaatacgga cgggtgccac
       61 gtgagttcgc cgaccaacct tctcgcgtaa ggatcgacga gcagcttgtt cggattgaag
      121 cgatggccgc gctcgggatc ccacgggccg tgcacgcgat agccgtacag ctgcccggcg
      181 ccaacgttcg gaacgtggat acgccaaacg tgattcgagc agtcgctcac cgggagcctt
      241 cggctctcgc gcgacggctc ttcggcgtcg aacaggcaca gctcgacgcc gctcgcgtgc
      301 tgcgaaaaga gggcgaaagt gacgccgtca ccgaagaggt tcgcgccgag ggtggccgcg
      361 ttcccgcgcg cgagtgccgt gacttctcca cggagcctgc gcgccgagct cgcttcgcgc
      421 cgcggcacca tgacggcctc ggcgggcctt gcgtcgccga ggacgagcct ggccgtcccg
      481 ctgagcggcc ccgcgaacgc tgccgtcaaa gcggagaagg ctgacccagc ggtcgcgaag
      541 cgtgctttcg ggtcacgcgc aacacagcgc aggaaccacg catcgaaatc cgatggccag
      601 ggcgacggca ggccgagagc gcgagctcgc tgcgacggaa ccacgagcgg ctggtgcaat
      661 acctcggcgc aaagcgcggt gagcccgagc tccgggcgcg ccgccgcgag ccaatagttc
      721 tggcccgtga gcaaaaagaa cgcgactagc ccgaatgccc aaacgtcggt ttcgcgactg
      781 accgcgccgc ccgatatttg ctcgaatgcc atgaacgcgg gcgtgccgcg gatgctaccc
      841 gtagcggtaa cgctgccgtc gagcaccttg gcgacgccga aatccaggat ttttaccagc
      901 ggctcaccgt tgtcgcgcgt ggtcaagaat aggttttcgg gcttgagatc gcgatggatg
      961 atcggcgtcg cgcgcccggc tttgtcgatg aacccgtgtg cgtgatcgag cccctgcgcg
     1021 acttgagaga gcagccccag cgcttggctc gcggtgagcg caccctcctc tcgcaccacg
     1081 cgcagcaggg accgaccttc caacaactcc atggcgagga acggcgtgta agtcgccgcg
     1141 tccacgcccg cgtcgagcac ctgcacgatg aactcgctct tcacgcggcc ggcgaccttc
     1201 gcctccaacg cgagccacgc gacggcaacg tcggactcgg ggatgtgagg ccagagcacc
     1261 ttcagcgcca cgagccgttc cgtcggcagg tgctcggcga cgaagacgat cccgtgcccg
     1321 ccccgggcga gcaatcgttc aacacgatat cgctcgccga aaacgtaccc ctgccggaaa
     1381 ggaaacagcg attcggccaa ctcgattcca ctaccccatt cctcacacgc gagcagctcg
     1441 gttcgagcgc ccgccgattc accggcgggc tcgaactgac tttttgagcc ccgtcgtcgt
     1501 cgcggggggc caaactcgat gcgcgcgcgc gtgatcgcag gtccgtgagg catgcgatct
     1561 tctcagcatt gcgcgtgcca gctctcgacc cgcggagttc ggccgttcga gcttatccga
     1621 ggggccaatg ctctcgctgc tcggatctgg ttgagcaggg cagtgctcga gcgcgagcgt
     1681 ggtgagccgt ttggatctca atcgcaccca tgaagtacta ggggtcagtc tgcccgagcg
     1741 ctcggcgcag cgcccttcgt gccgtaacgg cacgcgaccg cgaagtcacg cggcgctcat
     1801 tgacgtgaac gcgacggcaa ctcgagcggg catggttgct gcaatcgact cgggcagcgc
     1861 aggaaaatgt cgaaggggcc tgcgccgtct cgaatcagct ccttccacgc caaccgcagc
     1921 tgacctcacg tcggaacagg actattcatg cccaacaatc tcacgagtgt cgcgaccgca
     1981 aagacgatca agacggcgct cacgccttcc cagcgcagcc tctcgaccac ggccgcgagc
     2041 gcagcgaata caccttttgc tttcccccgc ttcgacgagt tcgcgcggcg caacccattc
     2101 gtcggggcga aggtcggcgc cccgcaggcg ttggcaaccg gcaactgggc gcaagaatt
//
LOCUS       DASTKS010000143         3417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_469791, whole genome shotgun sequence.
ACCESSION   DASTKS010000143 DASTKS010000000
VERSION     DASTKS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469791"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1989)
                     /locus_tag="VFQ35_03045"
     CDS             complement(115..1989)
                     /locus_tag="VFQ35_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9929632.1"
                     /translation="MKSGWRLFLYALGQALLAGGLAAPAYAADPPPTATGRAAAPLGE
                     YERRVLDVELAKRRAEIDDAPEGKLISEVEIVTLEVFDESDPFPDFFNVFHVVSRERV
                     IRRELLLHRGDPYRRRRADESVRNLRSLPQLSLVVLVALKDPDPRRVRLLLIAKDIWS
                     LRLNWNAQLGPGGLTYLILNPSERNFLGTHLLVGGTFELFRSRYGVSGTLAHGRLFGT
                     RLRSAAQVGAYVNRASGSTEGSFGQFSFGLPKYSEAQRWSYGVSLQWDDGITRVNVAL
                     PHEPYPTSTSLPYDSGGVRLFSYRREAYYEVLQTTRSFGLRHKLDLTFGLEASRRVYR
                     SLVPDSIDPVQRQKFIDREIPIGDTRFAPFFQVSRYENDFLKTIEIEALGLQEEFRLG
                     PQAMLRAYVAGTEFGSTRDIAGLFSGLAYTTPLGNGLMRAVGTSVIEYANDGKHQGML
                     ALAARIASPRFPLGRLHFDAVLENRYQNYLRDRSFLGGDGRLRGYAASDTEVSGSLRG
                     SDLVAMNLEFRSRGINVLSFQCGLAAFYDAGGTASRFDALAVKHSAGVGLRVLFPTFD
                     RTVFRADWAFPLNPGYDHFPGALFFTAGQAFGVPKLGDCLSCVGELRPVTYGLSSASW
                     "
     gene            2115..>3417
                     /locus_tag="VFQ35_03050"
     CDS             2115..>3417
                     /locus_tag="VFQ35_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9929633.1"
                     /translation="MQVHPTPSATHLSIAGSVACVLGVAIGSGAVVAWGGALLVGLAI
                     ARAVTEVSVARIRAAGFEMLWRDSARSRRLGRGEVIRIEAELRNREGRAARFAMLRAV
                     ASPELAVRVEPEHGEVPGGGRLRVVVEIAARRVGYHAVHGLSLELRAGPGLFEIPLTF
                     ANPFGVEVLPRPYTRLLRSARGGRSARAAEQTRSKRGAGESLELRELRQHMPGDPFRR
                     IAWKASARRGQLLVREHEREEHEIVFLLVDAAVELGAGRMGDSALDHSIDEAASVAMR
                     HLARGDRVGLGIVAGRVLAWIPPGRGPSHGFKLLEALAHAATPVHSDRSALDESEVAF
                     RVLEHMRPLDPEAVGNVGPYEVDRIARRADQLRPRAPFPQAAVAATSARELALRSYLA
                     AFGIGSPLRPGPERSETDELLGQALTRLRSERPEPSLVYLWS"
BASE COUNT          599 a         1114 c         1151 g          553 t
ORIGIN      
        1 cacgttccaa caaagcacga cacacggccg tgccaagctt gccggtggcg ccggtcacca
       61 gaatcgctgt cgtatcgctc atggcactca agatgaacta cgagccgcgg gcatctacca
      121 cgacgcgctc gagagtccat acgtcacggg gcggagctca cccacgcagc tcaagcaatc
      181 accgagcttt ggcacgccga aggcttgccc ggcggtgaag aacaacgcgc ccgggaaatg
      241 gtcgtagccc gggttgagcg gaaatgccca gtctgcgcga aaaacagtgc gatcgaacgt
      301 gggaaacaaa acgcgtaagc cgacgcctgc ggagtgcttg accgcgagcg cgtcgaagcg
      361 cgaggccgtg ccacccgcat cgtagaacgc tgcgagccca cattgaaacg agagcacgtt
      421 gatgccgcga ctgcgaaact cgagattcat cgcgacgaga tccgagccac ggagggagcc
      481 gctgacttcg gtgtcggacg ccgcgtaacc tctcaaccgg ccatcgccgc ccaagaagga
      541 gcgatcgcgc aggtagtttt ggtagcggtt ctcgagcacg gcatcgaagt ggaggcgccc
      601 gagcggaaac cgcggcgatg cgatgcgcgc ggcgagcgcg agcatgccct gatgcttgcc
      661 gtcgttcgcg tactcgatga ccgacgtgcc gaccgcgcgc atcaagccgt ttccgagcgg
      721 cgtcgtatac gcgagtccgc tgaagaggcc ggcgatgtcg cgagtcgagc cgaactcggt
      781 acctgcaacg tacgcgcgca gcatcgcttg cggaccgagc cgaaactcct cctgaagacc
      841 gagcgcttcg atctcgatcg ttttcagaaa gtcgttttcg tagcgcgaca cctgaaagaa
      901 cggcgcgaac cgagtgtcgc cgattgggat ctctcggtcg atgaacttct ggcgctggac
      961 cgggtcgatc gagtcgggga cgagcgagcg atagacacgg cgactcgcct cgagcccgaa
     1021 cgtgagatcg agcttgtggc gtagcccgaa cgagcgcgtc gtctggagca cctcgtagta
     1081 agcctcgcga cgataggaaa agagcctgac gccgccgctg tcgtatggga gggacgtgct
     1141 cgttgggtat ggctcgtgcg gcaaggccac gttgacgcgc gtgatcccgt cgtcccactg
     1201 cagagaaacg ccgtacgacc agcgctgcgc ttcgctgtac ttcggcaagc cgaagctgaa
     1261 ctgaccgaag gacccctcgg tcgagccgct cgctcgattg acgtacgccc ccacctgcgc
     1321 cgcgctacgg agccgcgtcc cgaagaggcg cccgtgcgcc agcgtgccgc tcacgccata
     1381 acggcttcga aatagttcga acgtcccccc caccaacaaa tgagtgccga ggaagtttcg
     1441 ctcgctgggg ttcaggatga ggtacgtgag cccgcccggc ccaagctgcg cattccagtt
     1501 gagtcgcagg ctccaaatgt ctttcgcgat taggagaagc cgcacccgcc tcggatcggg
     1561 atctttcaac gcaaccaaca cgacgagcga gagctgcgga agcgagcgca agtttcgaac
     1621 gctctcgtcg gcgcggcgcc ggcggtacgg gtcgccccga tgcagcaaaa gctcgcgccg
     1681 gatgacgcgt tctcggctca cgacgtggaa gacgttgaag aagtccggaa acggatcgct
     1741 ctcgtcgaac acctcgagcg tcacgatttc gacttcgctg atgagcttgc cttccggggc
     1801 gtcgtcgatc tcggctcggc gcttggcgag ctccacatcg agcacgcgcc gctcgtattc
     1861 acccagcggg gcggcggctc tgcccgtggc agtcggcggc gggtcggcgg cgtacgcggg
     1921 cgcagcaaga cccccagcga gcagcgcttg cccgagcgcg tataggaaca atcgccaacc
     1981 cgatttcatg gctcgggtcg gccgcttggc ggggactgcc ccggagtgca tcgcaaagcc
     2041 aaatcaggct acaccgatgg cgaggaaaac gcgcgtgaat tccacttcgc gcttcggtgg
     2101 tattcttcgc cccggtgcag gtccatccga ctccctcggc cacgcatctc tcgatagcgg
     2161 ggagcgttgc gtgcgtgctc ggggtggcga tcgggagcgg cgccgtcgtt gcctggggag
     2221 gcgcgttgct cgtcggcctc gccatcgcgc gcgcggtgac cgaggtgagc gtggcgcgga
     2281 ttcgcgcggc ggggttcgag atgctctggc gcgattccgc gagatcccgc cgcctcgggc
     2341 gcggagaggt aattcgcatc gaggccgagc tacgcaatcg cgaaggccga gccgcgcgct
     2401 tcgcaatgct tcgcgcggtg gcctcaccgg agctcgcggt gcgcgtcgaa ccggagcacg
     2461 gcgaggtgcc tggcggcggc aggttgcgag tcgtcgtgga aatcgcggct cgacgcgtcg
     2521 ggtatcacgc cgtgcacggg ctttcgctcg agctccgcgc ggggcctggc ttgttcgaga
     2581 ttcccctcac cttcgcgaac ccgttcgggg tggaggtgct gccgcggccg tacacgcgcc
     2641 tcttgcgctc ggcgcgcggc ggacggagcg cgcgagcggc cgagcaaacg cgctcgaaac
     2701 gaggtgccgg cgagagcctg gagctgcgcg agttgcgcca acacatgcca ggcgatccgt
     2761 tccgtcgcat cgcctggaag gcctcggcgc gtcgcggtca gctgctcgtc cgcgaacacg
     2821 agcgcgaaga gcacgaaatc gtctttctgc tcgtggacgc cgccgtcgag ctcggcgcgg
     2881 gacgaatggg cgactcggcg ctcgaccata gtatcgacga ggccgcgtcg gtcgcgatgc
     2941 gccacctcgc tcggggcgat cgcgtcgggc tcggtatcgt cgccggacga gtgctcgctt
     3001 ggatcccgcc ggggcggggg ccgagccatg gcttcaagtt gctcgaggcg ctggcgcacg
     3061 ccgcaactcc ggttcacagc gaccgaagtg cgctcgacga aagcgaagtc gcgttccggg
     3121 tgctcgagca catgcgaccg ctcgatcccg aggcggtcgg aaacgttggg ccctacgagg
     3181 tcgaccgcat cgcgcgtaga gcagatcagc tccggccgcg cgctcccttc ccgcaggctg
     3241 cggtcgcggc aacttctgcg cgcgagctcg cccttcgctc ttacctcgcc gcttttggta
     3301 ttggctcacc cttgcgccca gggccggagc gcagcgaaac cgacgaattg ctcggccagg
     3361 cgctcacccg cttgcgcagc gagcgacccg agccctcgct ggtctatctg tggtcgc
//
LOCUS       DASTKS010000144         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_23708,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000144 DASTKS010000000
VERSION     DASTKS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23708"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFQ35_03055"
     CDS             <1..805
                     /locus_tag="VFQ35_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929634.1"
                     /translation="ATLDHMDKGVDISDAVDVLKSCRAANINFHIFSMIGFPEETEES
                     AETTFRFFVDNARVIDHPGKTFDIHPFGLELRTRYFSEAEQMGVFVPAAALGKEFTIG
                     VASGEWQNTRGLGAARVEELISSYISRLHRVYRTHHNCPGHLWPGFEEYAVLYADKYE
                     ARRFPYMTRLPDESDDRAFRVVRSGLTYVEAGRDRVTLLGHYGKLETSRTNFALLDVE
                     EEQVFAELAATLRGAVPAEHASTVVDLYREYVHTLVGQGFVTVQLSEAP"
     gene            complement(858..>2072)
                     /locus_tag="VFQ35_03060"
     CDS             complement(858..>2072)
                     /locus_tag="VFQ35_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA domain-containing protein"
                     /protein_id="HET9929635.1"
                     /translation="GVHVYGASSMGALRAAELSAFGMRGVGQVFEALASGELEDDDEV
                     AVAHAPASRGFQPLSEAMVNLRVGLRRAEREGIISTATHEALLPLVKAQFYPERSWAS
                     VMSLGEAAGLPRSELRALVHFARTKQPNAKREDAVALLELIGAELEQARRSPHRPNFT
                     FESSVFWERTRENMASHGVSDADERVHRISREAVRTHVRLTDSKFRELQRSALLDVLA
                     AREAARMEIEVDEARVRREAERFRRARRLLRADEARSFLESNHVSKEEFSDAMQREAL
                     VQALTERFDEELGASIERELKLQGRFGVALEELAQKLDGVGETGLRHPAPSDLNIDED
                     ALVTWYQARYQPILGSLDVHAVEAGFASRRELVNEILALYLAERFARSGRNKVKADER
                     ADFAGPLSERPE"
BASE COUNT          366 a          656 c          661 g          389 t
ORIGIN      
        1 ggcgacactg gatcacatgg acaagggtgt cgacatcagc gacgcggtgg atgtcctgaa
       61 gagttgtcga gcggcgaaca tcaacttcca catcttctcg atgattggct tccccgagga
      121 gacggaggag tcggccgaga cgaccttccg cttcttcgtc gacaacgcgc gtgtcatcga
      181 tcatccaggc aagaccttcg acatccatcc cttcggcctc gagctccgga cgcgctattt
      241 cagcgaagcg gagcaaatgg gtgtgttcgt gcccgccgca gcgttgggca aggaattcac
      301 gatcggggtt gcgagcggcg agtggcagaa tacgcgtggc ctcggtgcgg ctcgagtcga
      361 agagctgatt tcctcttaca tcagtcgtct ccaccgagtt tatcggactc atcacaactg
      421 ccccgggcac ctttggccgg ggttcgagga gtacgccgtc ttgtacgcgg acaagtacga
      481 ggctcgccgt ttcccttaca tgacgcgctt acccgacgag tccgacgacc gggcgtttcg
      541 ggtcgtgcgg agcgggctca cttacgtcga ggcggggaga gaccgggtca cgctacttgg
      601 acattacggc aagctcgaga cgtcgcgcac caatttcgcg ctcctcgacg tggaagagga
      661 gcaagtcttc gccgagctag cggctacgct ccggggcgcg gtgccggccg aacacgcgag
      721 caccgtcgtc gacctctacc gcgagtacgt tcacacgctc gtcggacaag gcttcgtgac
      781 ggtgcagttg agcgaagcgc cgtagcgcgg cgggcgtggg acgaagcgcc attgaatgga
      841 tggcgcctcg tccgcgttca ctccggtcgc tccgaaagag gacctgcgaa atcggcacgc
      901 tcgtccgctt tgactttgtt gcgcccactc cgggcgaagc gttcggccaa gtagagagcc
      961 aagatttcgt ttacgagctc ccggcggctc gcgaagccgg cctcgaccgc gtgcacgtcg
     1021 agcgagccca ggatgggctg gtaacgcgcc tgataccaag tgacgagcgc gtcttcgtcg
     1081 atgttgagat cgctcggggc agggtggcgt aggccggttt cgccgacgcc gtcgagcttt
     1141 tgcgcgagct cttcgagagc gactccaaac ctgccctgga gcttgagttc gcgttcgatg
     1201 gacgcgccga gctcttcatc gaatcgctcg gtcagcgctt gcacgagcgc ctcgcgctgc
     1261 atggcatcgc tgaattcttc tttcgagacg tggttgcttt cgagaaacga gcgggcctcg
     1321 tccgccctca aaagacggcg agcgcgacga aatcgctccg cctcgcgccg aacgcgcgcc
     1381 tcgtcgacct cgatttccat tcgcgccgcc tcgcgggccg ccagcacatc gagcagcgcc
     1441 gaacgctgca gttcgcgaaa cttcgagtcg gtcaagcgga cgtgggtgcg cacggcctca
     1501 cgtgaaatcc gatgaacgcg ctcgtctgcg tcgctcacgc cgtgcgatgc catgttctcg
     1561 cgtgttcgct cccagaacac ggaggactcg aaagtgaagt tcggacggtg tgggctgcga
     1621 cgtgcctgct cgagctccgc tccgatgagc tctagcagcg cgacagcgtc ttctcgcttc
     1681 gcgttcggct gcttggtgcg agcgaagtgg accaacgctc gtagctctga acggggcagc
     1741 cccgccgctt ctccgaggct catgaccgaa gcccaggagc gctcgggata gaattgcgct
     1801 ttgaccagag gaagcagcgc ctcatgtgtc gcggtcgaga tgattccctc gcgctcggcg
     1861 cgccgaagac cgacgcgcaa gttgaccatc gcctcggaaa gcggctgaaa tcctcgcgag
     1921 gctggagcgt gcgcgaccgc gacttcgtcg tcgtcttcga gctcgccgga tgcgagcgct
     1981 tcgaagactt gtccgactcc gcgcatcccg aacgcgctga gctccgcagc gcgcagcgcg
     2041 cccatgctcg aagccccata gacgtgtacg cc
//
LOCUS       DASTKS010000145         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_23827,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000145 DASTKS010000000
VERSION     DASTKS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23827"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFQ35_03065"
     CDS             complement(<1..437)
                     /locus_tag="VFQ35_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929636.1"
                     /translation="MALSEAQSGAAARPVAGRSAPSWIFFLYVVGLVLVYAGERILSG
                     LPKGAGAVSLVGLLCMLAATVLRFSPRFRSGGERRSIESLLAVLSVVGLVGVTVYFAT
                     SDFGVTKLGLDRLATEKRARVEEFARVLWMSLVVVSTLPMLFA"
     gene            complement(428..1147)
                     /locus_tag="VFQ35_03070"
     CDS             complement(428..1147)
                     /locus_tag="VFQ35_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929637.1"
                     /translation="MNQVLLIARRELFAYLRSPLGAVIIAGALLIDGLWFYYQATQKL
                     VSADILQQFIWVASGTTMAASLFLSMRLFAEERQTGTLVLLNTSPVRDWEIVLGKYLS
                     AFSVVLVLTACTGYLPALILVHGKISLGHVLVGYAGILLIGSAAISIGLFGSALARSQ
                     VVAIIVSAAILGALILLWVVARSVDPPLNRFFSALALHHENFRPFMAGRLELGAVAYY
                     VAMTYFFLLASMKVLEARRWR"
     gene            complement(1144..2166)
                     /locus_tag="VFQ35_03075"
     CDS             complement(1144..2166)
                     /locus_tag="VFQ35_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9929638.1"
                     /translation="MIQIQELYKYYGDRRAIGPLSIEVNKGEIVGLLGLNGAGKTTTL
                     RVLACDLMPTSGTVKVGGFDVVANPDEVRARVGYLPDRPPLYDDMAVGEYLRFAARLR
                     KVPSGEVERRVGDVLELTELGDVRGQMIGTLSHGYRQRVGIAQAIVHRPEFVVLDEPI
                     SGLDPVQIVEMRELVQSLRGDHTVIVSSHILSEISETCDRLLVIDNGRISWSGTEKQL
                     FAELGQGMALNVTVRGASQRALEVIGAVPGVQGVEGVPPSEAGDGILTLQVSANSDVR
                     DAVCRALVAANLPVLELVRRQGLESMVLKLLGGDDAAARRKNRKKRDVSLPETAESAE
                     VEAEER"
BASE COUNT          461 a          805 c          737 g          357 t
ORIGIN      
        1 tccgcgaaca acatcggcag cgtcgagacg acgacgagcg acatccacaa gacgcgcgcg
       61 aactcttcga cgcgcgcgcg cttttcggtc gcgagccgat ccaacccgag cttcgtgacg
      121 ccgaaatcgc tcgtcgcgaa atacacggtg acgccgacga ggccgacgac cgacaaaacc
      181 gcgagcaggc tttcgatgct tcgccgctca ccgccgctcc ggaagcgcgg agagaagcgg
      241 agcaccgtcg ccgcgagcat gcagagtaag ccgaccaggc tcaccgctcc cgcgcccttc
      301 ggcaacccgg acaggatgcg ctcgccagcg tagacgagca cgaggccgac cacataaagg
      361 aaaaagatcc agctcggcgc gctgcgacct gcgaccggtc gcgccgccgc gccagattga
      421 gcttcgctca gcgccatcgg cgtgcctcca gaaccttcat cgaagcgagc aggaagaagt
      481 atgtcatcgc gacgtagtac gcgaccgcgc cgagctccaa acgaccagcc atgaacgggc
      541 ggaagttctc gtggtgcagc gcgagcgccg aaaagaagcg attgagcggc ggatcgaccg
      601 agcgcgcgac cacccagagc agaatcaacg cgccgagaat cgcggccgac acgatgatgg
      661 cgaccacctg ggagcgggcg agcgcggagc cgaagagacc gatcgagatc gcggcgctcc
      721 cgatcaagag aattcccgcg tacccgacga gcacgtgacc caagctgatc ttgccgtgca
      781 cgaggatgag cgcgggcaga tacccggtgc acgcggtcag aacgagcacc acgctgaagg
      841 ccgacaggta ctttccgagc acgatctccc agtcgcgaac cggggacgtg ttgagcagca
      901 cgagggtgcc ggtttgacgc tcctcggcga acaggcgcat cgagaggaag aggctcgcgg
      961 ccatcgtcgt cccgctcgcg acccagatga actgttgcaa gatgtccgcc gagaccagct
     1021 tctgggtggc ttggtagtaa aaccaaaggc catcgatcag gagcgcaccc gcgatgatca
     1081 ccgccccgag cggcgagcgc aggtacgcaa agagctcgcg gcgagcaatt aagagcactt
     1141 gattcatcgt tcctccgctt cgacctcggc gctctccgcc gtctcgggca gcgaaacgtc
     1201 gcgcttcttt cgatttttac ggcgcgcggc cgcgtcgtcc ccacccaaga gcttgagtac
     1261 catgctctcg agcccttgcc gtcgcacgag ctcgaggacg ggcagattcg cggcgacgag
     1321 ggcacggcaa acggcgtcgc gcacgtcgct gttcgccgag acttgcaggg tgaggatgcc
     1381 gtctccggct tcgctcggcg gaacgccttc gacgccctgc acgccgggca ccgcgccgat
     1441 cacctcgagc gctcgctggg acgcgccccg caccgtaacg ttcagcgcca tcccctgtcc
     1501 gagctccgca aagagctgct tttcggtgcc ggaccaagaa atgcggccgt tgtcgatcac
     1561 gagcagccga tcgcacgtct cgctgatctc actcaagatg tgactcgata cgatgacggt
     1621 gtggtcgccg cgcaagctct gcacgagctc gcgcatctcg acgatttgca ccgggtcgag
     1681 gccagagatt ggctcgtcga gcaccacgaa ttcggggcgg tgcacgatgg cttgggcgat
     1741 accgacccgc tgccggtagc cgtgcgagag cgtgccgatc atctgcccgc ggacgtcgcc
     1801 gagctccgtg agctcgagca cgtcgccgac gcgccgctcg acctcgccgc tcgggacttt
     1861 gcgcaaacgg gccgcgaacc gcaggtactc gcccaccgcc atgtcgtcgt agagcggagg
     1921 ccggtctggc aagtagccga cgcgcgcccg cacctcgtcg gggttcgcga cgacgtcgaa
     1981 gccgccgacc ttcacggtgc cgctcgtggg catcaagtca caggccaaca cgcgcagagt
     2041 cgtggtcttg cccgcgccgt tcaggcccag caagccgacg atctccccct tatttacttc
     2101 gatcgagagg ggcccgatcg cccggcgatc tccatagtac ttatagagtt cctgaatttg
     2161 gatcacggcg ctcgcccttc cggtcccgcg gttaccacaa agtttcggcg ggtcgtacca
     2221 ggaactgcgc cggtttattc ccggaaagcg ccagtctccg tgcgcggcgc tcgtcccacc
     2281 ggccggcgcg gctgcgcgtc tgctacgact cgcgcttcca gccgcaaaac gacgacgagc
     2341 caactttgga cgatgaaccc
//
LOCUS       DASTKS010000146         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_244848, whole genome shotgun sequence.
ACCESSION   DASTKS010000146 DASTKS010000000
VERSION     DASTKS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244848"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..769)
                     /locus_tag="VFQ35_03080"
     CDS             complement(353..769)
                     /locus_tag="VFQ35_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9929639.1"
                     /translation="MVPSVRPQPLLAVRDVRASARWYATLLGAQRTSELMHSDHDHLY
                     DRLLVDGTLVLQLHAWDAEHHPNLVDPDGGRNGHGVLVWFELDDLDAAVARARSLHAR
                     VEEELHVNPAPGHREIWLRDPDGYLVVLCSPDGEAR"
     gene            1377..1751
                     /locus_tag="VFQ35_03085"
                     /pseudo
     CDS             1377..1751
                     /locus_tag="VFQ35_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
     gene            1811..2272
                     /locus_tag="VFQ35_03090"
     CDS             1811..2272
                     /locus_tag="VFQ35_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929640.1"
                     /translation="MQVSKLIHEEGGTLEQDLVRARELLRDAMAKIADFVTVLQESTA
                     HARELSQLSKESEQAGRLTQVLDSIQSEATSAILGLQVEDVLGQLIEQTQRRIAALSR
                     LSTTVAKVIDERPSLHPKDGKIHAHIAELEAERARVCVAQASLDSGDAELF"
     gene            complement(2301..3158)
                     /locus_tag="VFQ35_03095"
     CDS             complement(2301..3158)
                     /locus_tag="VFQ35_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET9929641.1"
                     /translation="MQSPALRRTMTSALLFDLDGTLTDPREGITRCVRHALESVGAPV
                     PNEEELARWIGPPLQDSLRAHLERQTVRNSLNVAENGSARSDRVGGVSSEFISEEGGG
                     ACRPHKPRLERRAVKLVGVLGPSGEGLVARALSEYRARFATTGMFENAVYPNIPEALE
                     DLQRQGLPLFVATSKPTPFAERIVEHFGLSRYFAGVYGSELSGERSDKGELIAHVLRA
                     ENLAPSRVSMVGDRAHDIVGARRNGVRALGVLWGYGDAAELVEAGADAIFATVRDLSL
                     ALRELHSQS"
     gene            complement(3158..3886)
                     /locus_tag="VFQ35_03100"
     CDS             complement(3158..3886)
                     /locus_tag="VFQ35_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HET9929642.1"
                     /translation="MAVPSVFEVVVPDSIWVCERPVWFGGVRLRSRTTVVRLAGGALW
                     VHGPSTPSEDLCAALDSLGEVRWLVVPNRFHHLETPATSARYPSATVVGPKSAESRNP
                     QVKLTMATNDGAYVRATPELTPIQLAGVPFLDETVFFHPATGSLIGADIVISACPRDH
                     WTWRTAARIWGRYQKVRTPPDVRWNTRASAAAAESIARMRALPLQRILVAHADPITER
                     PAEQLAEAWDFAIPSNLRQSSAVH"
BASE COUNT          734 a         1253 c         1271 g          663 t
ORIGIN      
        1 gccgagcttc acgtggggga acatcgcgct cagatcgtga atgtaaagct ccagcagatt
       61 gccgagcaac accgcgtgcg cggcggtcgc gcgctccagc gctacgtcaa tcgatcgcgt
      121 cattgaacag tgctactgtc gtcgacccct gccgccagct gctcgagaac agcaggatgc
      181 gcgcgccggt gtgctgcata tgccgccgca acttcgctcg tgtaaacgat tgtgctcatc
      241 gagtgcggcc cattcatcgc ttggccttcc gcgccgcgct ccgcgccgaa ccgtggacag
      301 cggaacacga ataagcgtat cgcgcaatcg cactgcctgc gcgtgttctc ctctaacgtg
      361 cctcgccgtc ggggctgcac aggactacga ggtagccgtc cgggtcccgt agccatatct
      421 cccggtggcc gggagccggg ttgacgtgaa gctcttcttc tactcgagcg tgcagtgagc
      481 gagctcgggc gaccgctgca tcgaggtcgt cgagctcgaa ccataccaag acgccatgac
      541 cgttacgccc gccgtcggga tcgaccaggt tcgggtggtg ctcggcatcc caagcgtgga
      601 gctgtaggac cagggtgcca tctaccagga gacgatcgta gaggtggtcg tggtcggaat
      661 gcatgagttc gctcgtgcgc tgcgcaccga gtaaagtcgc gtaccagcgc gcgctcgctc
      721 gaacgtcgcg tacggcgagc agaggctgag gtcgtacaga gggaaccatg cgcggatgct
      781 agcgcgtgcg agtcgtcgga tgaagcgccg tatcggctgc gccggcatgc gataatggct
      841 cgatctcgta cgatatattc actccaacgt attacagggc gctccccgtg gcggtacgag
      901 accactcgat tgttccttcg cgcccagcgt ggtgaagggc aaacaactac cttggctgct
      961 cgtgcgcgca gaccgtccag gcgagtccga gcgacaccgc gtcgagggac cacgcaaact
     1021 gacgcatgat ccgttaatgc ctctctgcca cctgccgtac gagaggcgaa gctgatgtca
     1081 gcgatttcgg cagcaatggc gtcggacatc aatgagcctc ggttggcgat gtcgggccgc
     1141 gaacctggcg cagccacctc ggtcgcgagc gctcccggcg ccctcgcgtg cggcggagcc
     1201 cgagcccgaa cagatcccgc accggcgtat cgtgctcggc gagtgggagg tcgccatcga
     1261 gcctaccatc atcagcacgt tgctcggctc gtgcgtgagc gcgtgcctgt tcgacccaca
     1321 gaagcgcatc ggtggcatga accacttcat gctcccgagc gggcggcggc cacagcgatt
     1381 ccgcagactt cggagtgaac gcgatggagc tcctcatcaa cgcgctcatg acgcgcggtg
     1441 ccgagcgtcg caacctgcgc gccaaggtct tcggtgcggg ggcagtgacc agcgcgctgc
     1501 cgccgaccgt cggcgacgcg aacgccgcct tcattcgcgc cttcctcgcg aaagaggcca
     1561 tccccctggt cgtggagcga ttgggcggga cccggccgcg cgaggtgttt ctccgaacgc
     1621 atacgggtga ggtgttggtg cgcgccatca acccgcaacg tgcccgagct gtcgaggaac
     1681 gcgagatcaa cgcgtggaat cggaaggaag cgaacgaacc tgcgttctcc gccgatgatg
     1741 cgctctttta gtgaaagaac ccatggcgaa ccccgagata gagaaaccga gcccaccgga
     1801 ctcggccata gtgcaagtca gcaaactcat tcacgaggaa ggcggtaccc tcgagcagga
     1861 tctcgtgcgc gcccgcgagc tgttgcgcga cgcgatggcc aaaattgcgg atttcgtcac
     1921 ggtcctgcaa gagagcaccg cgcatgcccg cgagctcagc cagctcagca aggagtcaga
     1981 gcaagcgggt cgcctgactc aggtgctcga ctccatccaa agcgaagcga cctcggccat
     2041 tctcgggctc caggtggagg acgttctcgg gcagctcatc gagcagacgc agcgccgcat
     2101 cgccgcgctg tcgcgcctca gcacgaccgt cgcgaaggtc atcgacgagc gcccgagctt
     2161 gcacccgaaa gacggcaaaa tccacgccca catcgccgag ctcgaggcgg aacgtgcgcg
     2221 cgtgtgcgtc gctcaggcct cgctcgattc gggcgacgcc gaactcttct gacgcaaagt
     2281 tgctgcagaa gggtgcaggc ctagctctgc gagtgaagct cgcgcaaagc gagggacaga
     2341 tcgcgaacgg tggcgaagat cgcgtcggct cccgcttcga cgagctcggc cgcgtcacca
     2401 tatccccaga gcactcccag cgcgcgtacg ccattgcgcc gcgcgccgac gatgtcgtgc
     2461 gctcgatctc cgaccatcga tacgcggctc ggcgcgaggt tttcggcgcg cagcacgtgg
     2521 gcaatcagct cgcctttgtc ggagcgctcg cccgataatt cggacccgta gacaccggca
     2581 aagtacctcg acaagccgaa gtgctcgacg atgcgctccg caaagggagt cggtttggag
     2641 gtcgcgacga acaggggtaa gccctgcctt tgcaggtcct cgagggcctc gggtatgttc
     2701 gggtagacgg cgttttcgaa catacccgtc gtcgcgaagc gagcccggta ttccgaaagc
     2761 gcgcgcgcaa ccaaaccctc gccggaaggt cctagaacgc cgaccagttt gactgctcgg
     2821 cgttctagcc ttggtttgtg ggggcggcac gcgccgcccc cctcttccga aatgaattcg
     2881 gaagagactc cccccacgcg gtcactccgt gcggagccgt tttccgctac gttcagcgaa
     2941 ttcctaaccg tttgtcgctc taggtgggct cgcaacgagt cttggagtgg cggaccaatc
     3001 cagcgcgcga gttcttcttc gtttggaacg ggcgcgccga cggactcgag tgcgtgtcga
     3061 acgcacctcg taatgccctc ccgaggatcc gtcagcgtcc cgtcgaggtc gaatagcagc
     3121 gccgacgtca tcgttctgcg caaagcgggc gactgcatca gtgcacggcg ctcgactgac
     3181 gcaggttgga ggggatggcg aagtcccagg cctccgcaag ctgctcggcg gggcgctccg
     3241 tgattgggtc ggcgtgcgct accagaatcc gctgaagcgg cagggcgcgc attcgcgcga
     3301 ttgattcggc cgcggccgcg ctcgcgcgag tattccagcg gacgtccggc ggcgttcgga
     3361 ccttttggta gcgaccccag atgcgagcgg ccgttcgcca agtccagtgg tcgcgcgggc
     3421 aggcggaaat gacgatgtca gcgccaatga gcgagccagt cgccggatgg aagaagacgg
     3481 tctcgtcgag gaaaggaacg cccgcgagtt gtatgggtgt aagttcggga gtcgcgcgga
     3541 catacgcccc atcgttcgtg gccatggtga gcttgacctg ggggttccgc gactcggcag
     3601 actttggtcc aacgacggtg gcgctggggt atcgagccga cgtggccgga gtctcgagat
     3661 ggtggaatcg attgggcacg acgagccaac gcacttcgcc gagcgaatcg agggccgcgc
     3721 acagatcttc gctgggggtg gacgggccat gaacccaaag cgcaccgccc gcgagtcgaa
     3781 ccacggtagt cctcgaacgc aggcgaacac cgccgaacca caccggacgc tcgcacaccc
     3841 atatggagtc cggcaccacc acctcgaaca cgcttggaac cgccatggca ggaccttact
     3901 ctagcctgcg gagcctgcga a
//
LOCUS       DASTKS010000147         6203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_24088,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000147 DASTKS010000000
VERSION     DASTKS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24088"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFQ35_03105"
     CDS             <1..625
                     /locus_tag="VFQ35_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929643.1"
                     /translation="PFASGVGDSPSVNPAFVFDVPAELDAVETPRFALLGSVCANGVG
                     SATGCASGDELPVSLDLFFAATETTNENPSIPGDAIALDDVTWSPGEPPTGACSGLGL
                     PEVSLSAGQHQIQISLPESVREALPQHSSADAPREEIRFSHFASAGELERPFSAVLPG
                     DASVTTKLSWKPPKSVASSGELVRFWFVVRDLRGGSDFTERALCLRP"
     gene            657..1292
                     /locus_tag="VFQ35_03110"
     CDS             657..1292
                     /locus_tag="VFQ35_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9929644.1"
                     /translation="MDITRITSLAQFGASWVLWLLVALSIVAVAVAIERAVLFLSSRD
                     DIVRLRDELRALLSRGDTGAAQRRLEESPSFEARVAAAGLNADGSASAEERIAGETEL
                     VRLTMEKNLTVLGTLGNNAPFVGLLGTVIGIVRAFRELEHSSGQVSAGLMSEIGEALV
                     ATAVGLVVALPAVAFFNFYQRVIRARLARAGALGHEILAFRKSELSPTAAE"
     gene            1296..1718
                     /locus_tag="VFQ35_03115"
     CDS             1296..1718
                     /locus_tag="VFQ35_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9929645.1"
                     /translation="MAHASSHNDEPISGINVTPLVDVVLVLLVVMMVTATYIASRSIP
                     VDLPSGKTGEAAQAPLAISVDKSGALYLDGTKVDEPSLRTRLAAARSKNKELRAVIAA
                     DGSVAHRQVVRVIDLLRQEHIVRFGINVNPDDLKPATR"
     gene            1726..2514
                     /locus_tag="VFQ35_03120"
     CDS             1726..2514
                     /locus_tag="VFQ35_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9929646.1"
                     /translation="MRWPSLPWLFWAFSSSFVLHGVAYASLSFAPRHARVASLQSEVT
                     FEVNEPPPPPLPLKEPEQEAPEEPPAKAEPKLARVEPSRPTPALRTPVATAPANEPKP
                     SAAPLDLSGVTLTNDEGPSFGMALGNGRAFEGPIGNGRAPVAAREAAMPSQPPLSNTA
                     PALVAAADLSQKPAPPALDAALRNNYPRDARERGIGGSARVRARVEPSGAVRQIRVES
                     ESFSGFGDACRKTLEGSRWSSPRDREGRAVATEIRYTCRFVVEP"
     gene            2511..4157
                     /locus_tag="VFQ35_03125"
     CDS             2511..4157
                     /locus_tag="VFQ35_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929647.1"
                     /translation="MSFRVKSLGRSFGAIAGALLVLAAAPARAEEPAPTPVPAPATKP
                     SALRVFVDVGASDLDPQRIRGAIARELSSNVELAESGDTPLRIVCTSDQRVSVFYKND
                     SGEELSRVVDLPQDAERKVEVIALMVGNLTRNEAAELLAALQPKPEAKPPEPAAPAPP
                     PPPARAAPKGPDTADPELGSGYLYAKQPGANASLYFPTAVLRDSDRRVLALEASAAYG
                     RVGALQGIGFSLGVLRVEHQVQGLALALGATRVDGPVRGVQLGAFYNEGYGNQVGLNL
                     SSGLIYQRATVTGIVASGLVALTKDVDGIGVAGVLDVGRNLRGVHVAGVAAIATGRVD
                     GVQIAGALQSSEEINGIAIAGAVNRAAATRGFAVSGAVNVTGDLDGVAISLVNVGGRV
                     RGAQVGLVNVATKDVRGYQLGLVNYAHDNGHVQLQAFGDTLLPLNAGLKFTTGFGYSE
                     FGFGTSGSANSGHGYVGLGVHFPVGRLSFDVGGRVSASTRDDPSTGGPERTDVHYLGQ
                     VGLRIVRGIELFGGGGARHGVLGKGAGQADPEFLGGVAFF"
     gene            4315..4914
                     /locus_tag="VFQ35_03130"
     CDS             4315..4914
                     /locus_tag="VFQ35_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929648.1"
                     /translation="MSVSDALLAPPTSFQGASDDAEAELIQRLLRAERAAVAEVYDEH
                     HSAVRAFARRLVGEATAAEDLVHEVFVSLPKAVKNFRGQSSLRTFLISIAVNHARHHV
                     RAATRRRSAFGKLEKEPNPEVQNPEQATERKQLAEALTRALDTLPLDQRVAFVLCEVE
                     ERTSVDAARIVGVPEGTMRTRLFHAKKKLRSELEKEGFR"
     gene            4911..5702
                     /locus_tag="VFQ35_03135"
     CDS             4911..5702
                     /locus_tag="VFQ35_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929649.1"
                     /translation="MSDDLLRRAAQALREESEDGDAGERFTRARVMAGLQQNAGRRRL
                     RLAFALPLAACFVAATAFGMKGQTTELWHKVTQALGFTHAEPAPRTKTTAAPQAAPKP
                     IQKPPVVEPPAPVEPPTVETPAPAIEAPAAVEPPRAAPSAVHVPRVAPSAPPPAAEPA
                     PAPVNPPPAPPEANDPAHELYRVAHQAHFVEHDFASALRAWDAYLAAAPSGRFAPEAR
                     YNRALCLVRLGRKDDAERALRPFAEGAYGTYRRDEAKALIEALQR"
     gene            complement(5725..>6203)
                     /locus_tag="VFQ35_03140"
     CDS             complement(5725..>6203)
                     /locus_tag="VFQ35_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9929650.1"
                     /translation="FGGRYDASVNLREVLEFVRAEALAVQASVTPTNTPQAAVIGIVV
                     SDAFEVFFDTLESSRKCQNLRENPRIALVVGWDEKVGRTVQIEGVADEPTGAELERLK
                     RPYFERFPDGVVRQSWPGITYVRVRPTWLRYSDFSGAEPRVVEYDASDLVAFAHAR"
BASE COUNT         1032 a         2169 c         2012 g          990 t
ORIGIN      
        1 gccgttcgcg agcggagtcg gcgactcgcc aagcgtgaat cccgcgttcg tgttcgacgt
       61 ccccgccgag ctcgacgccg tcgaaacccc gcgttttgcg ctgctcggca gcgtgtgcgc
      121 gaacggcgtg ggctcggcca ccggatgcgc ttcgggagac gagctcccgg tgagcctcga
      181 cttgttcttc gcggcgacgg agacgaccaa cgaaaatccg agcatccccg gcgacgcaat
      241 cgcgctcgac gacgtgacgt ggagccccgg tgaaccgccg accggcgcgt gcagcgggct
      301 cggactgccg gaagtctcgc tcagcgctgg ccaacaccaa atccaaatca gcttgcccga
      361 atccgttcgc gaagcgctcc cgcaacacag ctccgccgac gcgccccgcg aagaaatccg
      421 cttttcacac ttcgcgagcg cgggcgagct cgagcgaccg ttctcggcgg tgttgccggg
      481 cgacgccagc gtgaccacca agctcagctg gaaaccgccg aagagcgtcg cgtcgagcgg
      541 cgagctcgtg cgcttttggt tcgtcgttcg cgacctgcgc ggcggcagtg acttcaccga
      601 gcgggcgctt tgcctgcgcc cctgaccgta tgttcgagaa aggactgatt tagaccatgg
      661 atatcactcg tatcacgtcg ctcgcgcagt tcggcgcgag ctgggttctc tggctcttgg
      721 ttgcgctctc gatcgtcgcg gtcgcggtcg cgatcgagcg agcggtcttg tttctgtcat
      781 cgcgtgacga catcgtgcgg cttcgcgacg aactgcgcgc cctgctgagt cgaggtgaca
      841 ctggcgcggc ccagcgccgc ctcgaagaga gcccgagctt cgaagcgcgc gtggccgcgg
      901 cggggctgaa cgccgacggc agcgcgagcg ccgaggagcg catcgccggc gaaaccgagc
      961 tcgtgcgcct gacgatggaa aagaacctga ccgtgctcgg caccttgggc aacaacgcgc
     1021 cgttcgtggg gctgctcggc accgtgatcg gcatcgtgcg cgcgtttcgc gagctcgaac
     1081 actcgagcgg ccaagtctcg gcgggactga tgtccgagat tggcgaggca ctggtcgcga
     1141 ccgcggtcgg cctcgtggtc gcgctgcctg cggtggcctt cttcaacttc taccagcgcg
     1201 ttattcgcgc ccgcttggct cgcgctggcg cgctcgggca cgaaattctg gcgttccgta
     1261 aatccgagct ctcgcccacc gccgcggagt gaaccatggc tcacgcttct tcgcacaacg
     1321 acgaaccgat ttcgggcatc aacgtgacgc cactcgtgga cgtggtgctg gtgctcttgg
     1381 tggtgatgat ggtgaccgcg acgtacatcg cctcgcgctc catccccgtg gacctaccga
     1441 gcggcaaaac cggtgaggcc gcgcaagcgc cgctcgcgat cagcgtcgac aagagcggcg
     1501 cgctctacct cgacggcacg aaggtcgacg agccttcgct ccgaacgcgc ctcgcggccg
     1561 cgaggagcaa gaacaaggag ctgcgcgccg tgatcgccgc cgacgggagc gtggcgcacc
     1621 gacaagtcgt ccgcgtgatc gatctcttgc ggcaagagca catcgtgcgc ttcggaatca
     1681 acgtcaatcc cgatgacctc aaacccgcta cccgctgagc aactcatgcg ctggccgagc
     1741 ttaccctggc tattttgggc gttttcgtcg agcttcgtgc tgcacggcgt cgcgtacgca
     1801 tcgctctcgt tcgcgccacg tcacgcgcgc gtggcttcgc tccaaagcga ggtcacgttc
     1861 gaagtcaacg agccacctcc gccgccgttg cctctgaaag agcccgagca agaggctccg
     1921 gaggagccgc cggcaaaagc ggaacccaag cttgcgcgcg tcgagccctc gcgcccgacg
     1981 cccgcgctgc gaacacccgt cgcgaccgcg cccgcgaacg aaccgaagcc gagcgcagcg
     2041 ccgctcgact tgagcggcgt cacgctgacg aacgacgaag ggccgagctt cggcatggcc
     2101 ctcgggaacg gtcgcgcttt cgagggtccg atcggcaatg gccgcgcgcc cgtcgcggcc
     2161 cgcgaagccg cgatgccgtc gcaaccgccg ctctcgaaca cggcgcccgc gctggttgcc
     2221 gctgccgatc tgtcgcaaaa gccggcgccg ccagcgctcg acgccgccct ccgcaacaac
     2281 tatccgcgcg acgcccgcga gcgcggcatc ggcggcagcg cgcgggtcag agctcgcgtc
     2341 gagccgagcg gcgcggtccg ccaaatccgc gtcgaaagcg agtcgttcag cgggttcggc
     2401 gacgcctgcc gcaaaacgct cgaaggatcg cgctggagct cgccgcgcga tcgcgaaggc
     2461 cgcgccgtcg cgaccgagat tcggtatacg tgccggttcg tagtcgaacc atgagttttc
     2521 gtgtcaaaag cctcggccgc agcttcggcg caatcgccgg ggcgctgctc gtcctcgcgg
     2581 cggcgcccgc gcgagcagaa gagccggctc cgactccggt tccggctccg gcgacgaagc
     2641 cgagcgctct ccgcgtcttc gtcgacgtcg gcgcgagcga cctcgacccg cagcgcatcc
     2701 gcggagccat cgcacgcgaa ctcagctcga acgtcgagct cgcggagagc ggcgacacac
     2761 cgttacgcat cgtttgtact tctgatcagc gcgtttcggt cttctacaag aacgattctg
     2821 gcgaagagct ttcgcgcgtg gtcgacctgc cgcaggacgc cgagcgaaag gtcgaagtca
     2881 tcgcgctgat ggtcggcaac ttgactcgca acgaagccgc cgagctactc gccgcgcttc
     2941 aacccaaacc cgaagcgaag ccacccgaac ccgccgcccc tgcgccgccc ccgccccccg
     3001 cgagagctgc gccaaaaggc cccgacaccg cggatcccga gcttggcagc ggctacttgt
     3061 acgcgaagca gccgggcgcg aatgcttcgc tctactttcc caccgccgtg ctgcgcgaca
     3121 gtgaccgtcg cgtgctcgcg ctcgaagctt cagccgccta cgggcgcgtc ggcgcgcttc
     3181 aaggaatcgg cttttcgcta ggggtgctgc gcgtcgagca tcaggtgcag ggcctcgcgt
     3241 tggcgctcgg cgcgacgcgc gtggacggac cggtccgcgg cgtccaactc ggcgcgttct
     3301 acaacgaggg ctacggcaac caagtcgggc tgaacctgag ctcgggtctc atctatcaac
     3361 gcgcgacggt gaccggcatc gtcgcctcgg gcctcgtcgc cctgacgaag gacgtggacg
     3421 gcatcggcgt cgcgggcgtg ctcgacgtgg ggcggaacct tcgcggggtt cacgtcgctg
     3481 gcgtcgccgc catcgcgacc ggccgagtcg acggcgtcca aatcgccggc gcgctccaat
     3541 ccagcgaaga aatcaacggg atcgcgatcg cgggcgccgt caatcgcgcc gccgcgacgc
     3601 gtgggttcgc cgtcagcggt gccgtgaacg tgacgggaga tctcgacggc gtcgcgatca
     3661 gcctcgtgaa cgtcggcgga agggtacgtg gcgcgcaggt cggcctcgtc aacgtcgcaa
     3721 ccaaggacgt gcgcggctat caactcgggc tcgtgaacta cgcgcacgac aatgggcacg
     3781 ttcagctcca agccttcgga gatacgttgc tgccactgaa cgcgggactg aagttcacga
     3841 ctggcttcgg ctattcggaa ttcggatttg gaacgtcggg ctccgctaac tctggccacg
     3901 gttacgtggg gctcggagtg cactttcccg ttggtcgctt gtcgttcgat gtcggaggcc
     3961 gcgtctccgc gagtacgcgc gacgacccca gcacgggcgg ccccgagcgc accgacgtgc
     4021 actacctggg gcaggtcggg ctgcgcatcg tgcgcgggat cgagctcttc ggcggtggag
     4081 gtgcgcgtca cggcgtgctc ggaaaaggcg ccggacaagc cgacccagag tttctcggcg
     4141 gcgtggcgtt cttttgagaa acccccgtaa tgtcgcaaag ttacacgaat acggcggacc
     4201 gattgaacgc tcacgcggct gccgggcact cagattcgga gtcgtgcgtt gctcttcggc
     4261 gttctcttcc aatcatcaca ttcagccgct gcccaggcgg gattggattg ggctatgtct
     4321 gtgagcgacg ccttgctcgc tccccccacg tctttccagg gcgcttcgga tgacgctgaa
     4381 gcagagctca tccagcgctt gttgcgcgcc gaacgcgcgg cggtagcgga agtgtacgac
     4441 gaacaccaca gcgccgtgcg cgccttcgcg cggcgcttgg tcggtgaagc gacggcagcg
     4501 gaagacttgg tgcacgaagt gttcgtctcg ctccccaaag cggtgaagaa cttccgtggc
     4561 cagtcgtcgc tccgaacatt tctgatctcg atcgcggtga accacgcgcg tcatcacgta
     4621 cgtgccgcga ctcggcgccg ctccgcgttc ggcaagctcg agaaagagcc gaaccccgag
     4681 gtgcaaaatc cggagcaagc caccgagcga aagcagctcg cggaggcgct gacccgcgcg
     4741 ctcgacacgc tgccgctcga tcaacgcgtc gccttcgtgc tgtgtgaggt cgaagagcgc
     4801 acctcggtcg acgcggcgcg catcgtgggc gtgccggaag ggacgatgcg cacccggctg
     4861 ttccacgcca aaaagaagct ccgcagcgag ctcgagaagg aggggttccg atgagcgacg
     4921 acctcctccg ccgcgcggcg caagccctgc gagaagaaag cgaagacgga gacgccggcg
     4981 agcgcttcac gcgagcgcgc gtgatggcgg gcttacagca aaacgccgga cggcggcgct
     5041 tgcgcttggc cttcgcgctg cctctcgccg cttgcttcgt cgccgccacg gccttcggca
     5101 tgaagggcca aacgaccgag ctctggcaca aggtcaccca ggcgctcggc ttcactcacg
     5161 cggagccagc gccgcgaacg aagaccaccg ccgcgccgca ggccgcgccc aagccgatcc
     5221 aaaagccgcc cgtcgtggag ccaccggctc cggtcgagcc gcccaccgtc gaaacgcccg
     5281 cgcccgccat cgaggcgccc gcagccgtcg agccgccgcg cgcagcgcca agcgccgttc
     5341 acgtgccgcg cgtcgcgccg tctgcgccgc cgcccgctgc ggaaccggct cccgcgcccg
     5401 tgaatccgcc gcccgctccg cccgaagcga acgaccctgc tcacgagctc taccgagtcg
     5461 ctcatcaagc gcacttcgtg gagcacgact tcgcttctgc gctgcgagcc tgggatgctt
     5521 atctcgccgc cgccccgagt ggccgcttcg ctcccgaagc gcgctacaac cgcgccctct
     5581 gcctcgttcg gctcgggcgc aaagacgacg cggagcgtgc gctgcgtccg ttcgcggagg
     5641 gcgcgtatgg tacctatcgt cgcgacgaag cgaaggccct catcgaagcc cttcagcgct
     5701 gagtctatgc gcgaccgcgc ggcgctaacg cgcgtgcgca aacgcgacga gatcgctcgc
     5761 gtcgtactcg acgacgcgcg gctccgcacc actgaagtca ctgtaacgga gccaggtcgg
     5821 tcgcactcgc acataggtga tgccaggcca gctttgccgc accaccccgt cgggaaagcg
     5881 ctcgaagtag ggccgcttca agcgttcgag ctccgctccc gtcggctcgt ccgcgacgcc
     5941 ttcgatttgc acggtgcgcc ccaccttctc gtcccatccc acgacgagcg cgatgcgcgg
     6001 gttctccctc aggttctggc attttcgtga gctctcgagc gtgtcgaaga acacctcgaa
     6061 cgcatcgctc accacgatgc cgatcaccgc tgcttgcggc gtgttggtcg gcgtgactga
     6121 agcctgcacc gcgagcgcct cggcgcgaac gaactcgaga acttcacgga gattcacgct
     6181 cgcatcgtag cgcccgccaa aac
//
LOCUS       DASTKS010000148         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_692625, whole genome shotgun sequence.
ACCESSION   DASTKS010000148 DASTKS010000000
VERSION     DASTKS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692625"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFQ35_03145"
     CDS             <1..877
                     /locus_tag="VFQ35_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9929651.1"
                     /translation="HRDEGRHQGIDGREIEEGARLDLEDDALILRLSQRLRGPLTRNS
                     ASKEPVLYEHVLIDEAQDLSPVELAVVFGTVSKAQSITLAGDVQQRLLMDNGFTNWSE
                     VLGELGLSHVEVEPLKLSYRSTHEIIEFARDVLGPLSTPDAPEATRSGAPVELFTFAH
                     TGDAVAFLAEALRELLGEEPNASIAVITRFPEQADAYYDGLHRGEVPRLRRIADQDFP
                     FKAGVDVTDVRQVKGLEFDYVVLAEVNENSYPNEDESRHLLHIGATRSAHQLWVIATG
                     KPSPLLPETLLERSL"
     gene            880..1839
                     /locus_tag="VFQ35_03150"
     CDS             880..1839
                     /locus_tag="VFQ35_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET9929652.1"
                     /translation="MTRSLPRLASALFLCLACSPARAPSTPASLSGDEPRDVPEEVHE
                     PVPDDVVARAEGPVRVIRLSDGVELSSNAVARELLQYDAVCAGEEHTSAPQHYAELWL
                     LDRLGTQAPYLGLELGAGFEMWQTKDQGLVDAFADGTISEKRLLKRTDYKNTWGYDFA
                     YYRPILLRARELSLPVIALNASKTTTAKIRKGGLAALGEWKTSQLPELDLSDREHRAD
                     FERRMKKHPGVEAENLDNYYAAQVVWDETMANTSVRWLNRHTPVRRIFIVAGQAHCQR
                     SAIPKRVVRRGVPKAAALLLTTKRPSAEASKQYDYAVVVEASE"
     gene            complement(1864..2715)
                     /locus_tag="VFQ35_03155"
     CDS             complement(1864..2715)
                     /locus_tag="VFQ35_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET9929653.1"
                     /translation="MCRLFGFRSVIPSQVHRSLLAAENALGVQSNQHRDGWGVAFYVD
                     GAPHVTRSPLTAVGDALFHRLSGVVASQTVLAHVRRATHGEKTVFNCHPFQYGRWVFA
                     HNGDVARFGETREALLQAVSPALRRFILGETDSEVVFFIFLTELSAHGPLGGRFALDT
                     ALNAMRSTVQRVRSVCDRPDASLHSSLTLIATDGETLVATQGGKELFVSTYKTRCADR
                     DRCASLSASCEAPSTGTVNHLVLSSEPLHGENVWLPLEPGEMIGIDAGMRPLRSHIER
                     SRLPLAV"
     gene            2895..3611
                     /locus_tag="VFQ35_03160"
     CDS             2895..3611
                     /locus_tag="VFQ35_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9929654.1"
                     /translation="MRKQAVNGDLERLRARREFVKRAASGTALVALGGISYVISDDLT
                     RVARAETLPDGRSRVPPGQRVIHALKPMGGEPGDPDPARLKLRVHGAVEKPLELGFAE
                     FLKLPQVERALDVHCVTGWTVLGARFEGVLLKTLLDLARPTPKARYLIWEGAHGYTSN
                     MPLREALAPNVMVAQRLAGAALPRAHGGPLRAVVPQLYFWKSAKWLEGIRLSERDEPG
                     YWEVRGYNNHADPWREERYA"
     gene            3604..4182
                     /locus_tag="VFQ35_03165"
     CDS             3604..4182
                     /locus_tag="VFQ35_03165"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET9929655.1"
                     /translation="MPEPQKRRASVRPWLRALHRDIGYLAVGFTFIYALSGLAVNHIA
                     DWDPNFRQVSRVHELHLNLDGGDEPVSKRVLDALSIRELPRELYRASDSRLEIVLAQR
                     TLHVDTQRGVVEEEGQKPRFFLRLANFLHLNRGKKAWTYVADTYAVFLLFLASSGLFM
                     IPGKKGIWGRGAVFLAVGVLVPALYVVLAKTP"
BASE COUNT          820 a         1477 c         1446 g          731 t
ORIGIN      
        1 ccaccgcgac gagggccgac atcaaggtat cgacggacgg gagatcgaag aaggcgctcg
       61 cctcgacctc gaggacgacg cgctgatcct tcgcctcagc cagcgtttgc gcggccccct
      121 cacccggaac tcggccagca aagagcccgt gctctacgag cacgtcctga tcgacgaagc
      181 gcaagatttg agcccggtcg agctcgcggt cgtgttcggg acggtcagca aggcgcagag
      241 catcacgctg gcgggcgacg tgcagcagcg gctgctgatg gataacggct tcacgaactg
      301 gagcgaggtg ctcggcgagc tcgggctgtc gcacgtcgag gtcgagccgc tcaagctgag
      361 ctaccggtcg acccacgaga tcatcgagtt cgcgcgcgac gtactcggtc cgctcagcac
      421 ccccgacgcg cccgaagcga cccgcagcgg cgctccggtc gagctcttca cgttcgcgca
      481 cacgggcgac gcggtcgcct tcctcgccga agccctgcga gagctcttgg gagaagagcc
      541 caacgcctcg atcgcggtga tcacccgctt tcccgagcag gccgacgcct actacgacgg
      601 cttgcaccga ggcgaagtgc cgcggctgag gcgcatcgcc gaccaggact tccccttcaa
      661 ggcgggcgtc gacgtcaccg acgtgcggca ggtgaagggc ctcgagttcg actacgtggt
      721 gctggccgaa gtgaacgaga actcgtaccc gaacgaggac gagtcgcgtc acctcctcca
      781 catcggcgcg acgcgctccg cgcatcaact ctgggtcatc gccaccggaa agccgagccc
      841 gcttttgccc gagacgctct tggaaagaag cctgtaatca tgacccgatc gttgccgagg
      901 ctcgcctcgg cgctcttcct ttgtcttgca tgcagtccgg cgcgcgcacc gagcacgccc
      961 gcgtcgctct ccggagacga accgcgtgac gtcccggagg aggttcacga gcccgtgccg
     1021 gacgacgtcg tggcgcgcgc ggaaggcccc gtacgcgtca ttcggctgtc cgatggcgtc
     1081 gagctatcga gcaacgcggt cgcgcgcgag ctcttgcagt acgacgcggt ctgcgcgggc
     1141 gaagagcaca cctcggcccc gcagcactac gcggagctct ggctactcga tcgactcggg
     1201 acgcaagctc cctacttggg gctcgagctc ggtgccggct tcgagatgtg gcagacgaaa
     1261 gatcaagggc tcgtcgacgc cttcgcggac ggcacgatca gcgaaaagcg gctcctcaag
     1321 cgcaccgact acaagaacac gtggggctac gacttcgcgt attaccggcc gattttgcta
     1381 cgcgcccgcg agctctcgct tccagtcatc gcgctcaacg cgtcgaagac gacgaccgcc
     1441 aagatccgca agggtgggtt ggcagccctc ggtgagtgga aaacgagtca gcttcccgag
     1501 ctcgatctgt cggaccggga gcaccgcgcc gacttcgagc gccgcatgaa gaagcacccc
     1561 ggcgtcgagg ccgagaacct cgacaactac tacgccgctc aggtggtgtg ggacgagacg
     1621 atggccaaca cgagcgtgcg ttggttgaac cgtcacacgc cggtgcgccg catcttcatc
     1681 gtcgcggggc aagcgcattg ccagcgcagc gcaatcccga agcgcgtcgt acggcgcggc
     1741 gtacccaaag ccgcggccct gttgctcacc accaagcgac cgagcgccga agcgtcgaag
     1801 caatacgact acgcggtcgt ggtcgaagcg agcgagtagc ccgcgaaaag agctgccctc
     1861 gcctcaaacc gcgaggggaa gccggctgcg ctcgatgtgc gagcggagcg gtcgcatacc
     1921 cgcgtcgatg ccgatcattt cgccgggctc gaggggtagc cacacgtttt cgccgtggag
     1981 cggctcgctc gaaagcacga ggtggttcac ggtcccggtg ctcggcgctt cgcacgaggc
     2041 ggagaggctc gcgcagcgat cgcgatccgc gcagcgagtc ttgtaagtcg acacgaacag
     2101 ctctttaccg ccctgagtcg cgaccagcgt ctcaccgtcg gtggcgatga gcgtgagcga
     2161 agagtgcagg cttgcatccg ggcgatcgca caccgagcgc acgcgttgca cggtgctgcg
     2221 catggcgttg agcgcggtat cgagcgcgaa gcgcccgccg agaggcccat gggcagagag
     2281 ctcggtgagg aagatgaaga acaccacttc gctatcggtc tcgccgagaa tgaagcggcg
     2341 cagcgccggc gaaacggctt gcagcaacgc ttcgcgcgtc tctccgaagc gcgcgacgtc
     2401 tccattgtgc gcgaacaccc agcgaccgta ctgaaacggg tggcagttga agaccgtttt
     2461 ctcgccgtgt gtcgcgcgac ggacgtgagc gagcaccgtt tgggacgcaa cgacgccgct
     2521 caatcggtga aacaacgcat cgcccaccgc cgtgagcggg ctgcgcgtga cgtgcggtgc
     2581 gccatcgacg tagaacgcca ctccccaacc atcgcggtgc tggttgctct gtacgccgag
     2641 cgcgttctca gccgccaaaa gcgagcgatg gacctgactc gggatcacac tgcgaaagcc
     2701 aaaaagccga cacatgaatt agaagccggt cgaaacttct gttcagcctg ccacaacggc
     2761 cgcgccgccg catacttgat acttgctcgc gaattgcgaa ttctctcgct cgcgagtgcg
     2821 aattccgacc tgggggccgg cacccccgag agtaaccgtt tgagcgagtg ccgcgtagtg
     2881 gactccacga agccatgcgg aaacaagcag tgaatggtga cctggagcgg ctccgcgccc
     2941 gccgtgagtt cgtgaagcgc gctgcgagcg gcaccgcgct cgttgctttg ggcggcatca
     3001 gctacgtgat ctccgatgat ctcactcgcg tggcgcgcgc agagactctg cccgatgggc
     3061 gcagccgcgt gccacctggg cagcgcgtca tccacgcgct caaaccgatg gggggtgaac
     3121 ccggagatcc cgatcccgcg cggctcaagc tgcgtgtgca cggcgcggtg gagaagccac
     3181 tcgagctcgg ctttgcggag ttcttgaagc tgccgcaggt ggagcgagcg ctcgacgtgc
     3241 actgcgtaac gggatggacg gtgctgggag cgcgcttcga aggcgtgctc ctcaaaacgt
     3301 tgctcgacct cgcgcgaccg actccgaagg cgcgctacct gatttgggaa ggcgcgcacg
     3361 ggtacacgtc gaacatgccg cttcgcgaag cgctcgcccc gaacgtgatg gtcgcgcagc
     3421 gcctcgcggg agccgcgttg ccgcgagccc atggcggacc gttgcgcgcc gtggttcccc
     3481 agctctactt ttggaagagc gcgaagtggc tcgagggcat tcgcctgagc gagcgcgacg
     3541 agcccggcta ctgggaagtc cgcggctaca acaatcacgc cgatccctgg cgcgaagagc
     3601 gctatgcctg agccgcagaa gaggcgggcg agcgtgcggc cgtggctgcg ggcgttgcac
     3661 cgagacatcg gctatctcgc cgtcggcttc acgttcatct acgcgctctc ggggctcgcg
     3721 gtaaaccaca ttgccgattg ggacccgaac ttccgtcagg tgtcgcgcgt ccacgagctc
     3781 cacctgaatc tcgacggcgg cgacgagccc gtttccaagc gcgtgctcga cgcgctctcg
     3841 atccgagagc tgccgcgcga gctctaccga gcgagcgact cgcgcctcga aatcgtgctc
     3901 gcgcagcgga cgctccacgt cgacacgcag cgcggcgtgg tcgaagaaga agggcagaag
     3961 ccgcgctttt tcctgcggct cgcaaacttc ttgcatctca atcgcggcaa gaaggcttgg
     4021 acctacgtcg cggacacgta cgccgtgttc ctgctcttct tggcgagttc gggcctattc
     4081 atgatccccg gcaagaaggg gatctggggg cgcggagcgg ttttcctcgc cgtgggcgtg
     4141 ctcgtcccag ccctgtacgt ggtgctcgca aagacccctt gagcgcgccg cactgcgcca
     4201 atcgcgcgcg agggtcgcgt aaaatcacgg ccaacaggct tcgagcgctc atccgtgctc
     4261 aaattcatcg ggaaaaccaa tgtggaactg ttcccacatt tgcagcttcc ctgttgctgt
     4321 agaccctggt atgaaggcgc ccgcgtttgc ctaccttacc tccattcggc ggtcaggacg
     4381 gcacgaccca cggaagcgag ctacctacga tgcgcagcag caccctaccg atccttatct
     4441 ccggattgtc tggcttgttc gtcgccctcc ccgc
//
LOCUS       DASTKS010000149         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_470476, whole genome shotgun sequence.
ACCESSION   DASTKS010000149 DASTKS010000000
VERSION     DASTKS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470476"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFQ35_03170"
     CDS             <1..354
                     /locus_tag="VFQ35_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929656.1"
                     /translation="ASKGPRLVELTSYHGSGDTLSTATFRASPGRSCTAEHDGVLTAC
                     DRARRSAMLAAFVSQRQVLRVFGNEREPLRCAPQYDFTEPPAAPRHYERLPFGWTGER
                     FAHLARGALSQLGLA"
     gene            424..2577
                     /locus_tag="VFQ35_03175"
     CDS             424..2577
                     /locus_tag="VFQ35_03175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9929657.1"
                     /translation="MRRLTVLSIAYPFAPVSRDSPGGAEQILSILDQELVRAGHRSIV
                     IAREDSNVHGELLALPREAEPLDANAVARAHATLMRQTAGVIEGERVDLTHLHGIDFA
                     DYLPPPGHPVLATLHLPPTWYPRAALAPTHPELELCCVSDAQHAAMPLWAADARVVPN
                     GVRLDAFRARRRKSKFCLMLARICPEKGVHDALDAATEAEVPLWLAGEISSWPAHRSY
                     FEQEVAPRLRGGHRYLGVVEGARKRRLLSAARAVLIPSRVPETSSLVAMEALASGTPV
                     IAYRSGALPSVVDHEVTGFVVDDVQSLANGIRRAAELDPAVCRSAAESRFDARAMSAK
                     YLSLYEELAPARPRSTSSPSGNELRLERVAGLRALELLAPDWSRLCQRVPGASPFQTP
                     EWLLAYASTFCAEDGDAEPWALVARSREALVGVLPLCTRRTGNMVTTGLLGDGLSDYL
                     DMVAERTLAIRCAESFFEELVSELGPNARLVFDQLRKDSPLRATRAPAGVREQVAERD
                     VCPVVHFQTGLDPIPPNLRERIRYCRRRAERMGEVTIVAPRGSELTSWLETLFELHGS
                     RWSLRGESGVLATEEVRRFQRLAVPALVDVDAARIFGMSIGAERAAALLVLTDRSAAY
                     YYIGGFAPRFAALSPGTLLIAHVMDWARERGLLELDFLRGREPYKYRFGAHDRSSRSR
                     TFEGSDAREAASGTLKAEAAPGTLASSGAEIRHSA"
     gene            2631..>3040
                     /locus_tag="VFQ35_03180"
     CDS             2631..>3040
                     /locus_tag="VFQ35_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929658.1"
                     /translation="MNRRFRALTSSFSLALALGCQPLPANGPGPGADSGPPPIGVPPG
                     LPEGSWLALDASSDGPRPEQMKLDDAVVATFFGDDIPRASGGYTYAYGGKTANRVLAS
                     TTANNRGVFATYLDNDYSGVNISLGSTFVDLRPYR"
BASE COUNT          476 a         1019 c          993 g          552 t
ORIGIN      
        1 gcctccaaag ggccgaggct cgtggaactg acttcctatc acggttcggg cgataccctc
       61 tccaccgcaa cgtttcgcgc ctcgccgggg cgttcttgca ccgccgaaca cgatggagtc
      121 ctcacggctt gcgatcgtgc gaggcggagc gccatgctcg ccgccttcgt atcgcaacgg
      181 caggtgcttc gggtcttcgg caacgagcgc gagccgctgc gctgcgcgcc gcaatatgat
      241 ttcactgaac cgcccgcggc gccgcgacac tacgaaagat tgccgttcgg ttggaccggc
      301 gagcgctttg ctcatctagc tcgaggcgcg ctgtcccagc tcgggttggc gtgattcggc
      361 tcggccgagc gtgacgtcga ggccaccggt gcagaggcat gcggcgtgca cgcgcgcgac
      421 gcgatgagaa ggcttaccgt tctctcaatt gcgtatccgt tcgcccccgt atcccgcgac
      481 agccccggtg gggcagagca gatcctgtcc atcctcgatc aggagctcgt gcgggccggt
      541 caccggtcga tcgtgatcgc ccgcgaagac tcgaacgtgc acggcgagct cttggccctg
      601 ccacgcgaag cagagccgct cgacgcgaat gccgttgcac gggctcacgc gacgttgatg
      661 cgacagacgg cgggagtaat cgagggcgaa agagtcgatc tcacccacct gcacggcatc
      721 gatttcgccg actacctacc tccgccggga cacccggtgc tcgcgacgtt gcatctgccg
      781 cccacctggt accctcgggc agcgctcgcg ccgacgcacc cagagctcga gctgtgctgc
      841 gtttcagacg cccagcatgc cgcgatgccg ctttgggccg cggacgctcg cgtcgttccc
      901 aatggagtgc gcctcgatgc ctttcgagcg cgccgcagaa aaagcaagtt ctgtttgatg
      961 ctcgcgcgca tctgtcccga aaagggggta cacgatgccc tcgacgcggc gaccgaagcg
     1021 gaggtgccgc tctggctcgc gggagaaatc tcttcttggc cggcgcatcg aagctacttc
     1081 gaacaagaag tcgcgccgcg tttgcggggc gggcaccgct acctcggggt cgtcgagggc
     1141 gcacgcaagc gacgccttct gtcggcagcg cgggccgtgc tcattcccag tcgcgtgccc
     1201 gaaacgtctt cgttggtcgc gatggaggcg ctcgcgagcg gaacgccggt aatcgcgtat
     1261 cgctcgggcg cgctgccgag cgtcgtcgac cacgaggtga ccggctttgt cgtcgacgac
     1321 gtccaatccc tcgcgaacgg cattcgacga gcggcggagc tcgatcccgc tgtctgccgc
     1381 tccgcggccg agtcgcgctt cgacgcgcgc gccatgagcg ccaagtacct ttcgctttac
     1441 gaggagctcg cccccgcgag gccgcgatcg actagctcgc ccagtggcaa cgagctccga
     1501 ctagaacggg tcgcggggct ccgtgcgctc gagctcctgg ctcccgactg gagtcgcctc
     1561 tgtcagcgag ttcccggtgc ctcccccttt cagacaccgg agtggctcct ggcgtacgcc
     1621 tcgacgtttt gcgccgagga cggcgacgcg gagccctggg cgctggtagc tcgcagtcgg
     1681 gaagcgctcg ttggcgttct gcccctctgc acacgccgca ccggcaatat ggtcacgacc
     1741 gggctcttgg gcgacgggct cagtgactat ctcgacatgg ttgctgaacg cactctggcg
     1801 attcgatgcg cggagtcgtt tttcgaggag ctcgtctcgg aactcgggcc gaatgcgcgc
     1861 ctcgtcttcg atcagttgcg aaaggattct ccgctccgcg cgacgcgagc gccggcgggc
     1921 gtgagagagc aagtcgccga gcgcgatgtc tgtcctgtgg ttcacttcca gaccgggctc
     1981 gaccccattc cacccaacct ccgcgagagg attcggtact gtcgacgacg cgcagagcga
     2041 atgggcgagg tgacgatcgt cgcgccccga gggtcggagc ttacctcctg gctcgaaacg
     2101 ctgttcgagc ttcatggttc gcgctggtcg ttgcgcgggg agtccggggt gctcgctaca
     2161 gaagaagtcc gacgttttca gcgcctggcc gtaccggctc tcgtcgacgt ggacgcggca
     2221 aggattttcg gtatgagcat cggcgcggaa cgcgctgcgg cgctgctcgt gctgacggat
     2281 cgcagcgcgg cttactacta catcggtggg tttgctccgc ggttcgcggc tctgagccca
     2341 gggactctct tgattgcaca cgtcatggat tgggctcgcg agcgggggct tctcgagctc
     2401 gacttcctgc gcggaaggga gccttacaaa taccgtttcg gagcgcacga ccggtcgagc
     2461 cgaagccgaa ccttcgaggg gagcgacgct cgggaggccg cgtccggcac gctgaaagcg
     2521 gaagcggctc ctggcacgct cgcaagctcc ggcgcagaga ttcgtcactc ggcgtagcgc
     2581 cacggttgat cgcgcgatca gaactccgag cgcgtcccat ccaaggcgcg atgaatcgac
     2641 gttttcgcgc tctgacttcc agcttctcgt tggctctggc gctcggctgc cagccattgc
     2701 cggcgaacgg ccctggccca ggtgccgact cggggcctcc gcccatcggt gttcccccgg
     2761 gcttaccgga ggggagctgg ctcgccctcg atgcctcctc cgacggccct cggccggagc
     2821 agatgaagct cgacgacgcc gtggtcgcga ccttttttgg tgacgacatc ccgcgggcaa
     2881 gcggcggcta cacgtacgca tacggcggca aaactgcgaa ccgcgtgctc gccagcacga
     2941 ccgcgaacaa tcggggcgtg ttcgctacgt acctcgacaa cgactattcg ggcgtgaaca
     3001 tctcgctcgg cagcaccttc gtcgaccttc gcccgtaccg
//
LOCUS       DASTKS010000150         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1585124, whole genome shotgun sequence.
ACCESSION   DASTKS010000150 DASTKS010000000
VERSION     DASTKS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585124"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFQ35_03185"
     CDS             <1..506
                     /locus_tag="VFQ35_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929659.1"
                     /translation="MAVELKTLKRGKHESIGGVTFAAAPKHFALKVTRQNEALYDGDV
                     VPHYSPYPTKKEENKRFCGEQAFVQPKCVRGSSECAPFPTSCDGPEDCGEHEICCGSP
                     ESARDYGKDFATECSGRRRCLERYGHVACHKDSDCRSDETCADDGTAADFKPPLLACR
                     PRAEGAH"
     gene            699..1196
                     /locus_tag="VFQ35_03190"
     CDS             699..1196
                     /locus_tag="VFQ35_03190"
                     /inference="COORDINATES: protein motif:HMM:NF025077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HET9929660.1"
                     /translation="MTKAVSLSLAALLSVGCVFNSVAKLDPKAESVVVVHETDKPTDC
                     KYLGKIVGRSHADNEKQAKQGAENDLRNQAAKLKANFALIETQRGGPVGTTSQREVVM
                     NGKALFCKTLEMQQAEEAKHEQEMKEKEDREIREQAEKERKAEEEKEKKEREEQERKD
                     KKSKD"
     gene            complement(1293..1949)
                     /locus_tag="VFQ35_03195"
     CDS             complement(1293..1949)
                     /locus_tag="VFQ35_03195"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9929661.1"
                     /translation="MSKDNSHSDAGVPPAMRPRKVPNQERSQSTVDALLDGLARILVE
                     AADAPESATTNAIAARAGVSVGSLYQYFPNKEALVAALLRRQARAEANFVLGRMAAAG
                     AHDLRSALRVAIDAAFEFRASDPALQDALLRELDRVPQFPALVEEARQAARGLGELLS
                     AFRAEIRRSDLELALHVVCNAAFSLTHRGLFPRPADVDEGRYRDEVVSLLTAYLTHPE
                     "
     gene            1975..2859
                     /locus_tag="VFQ35_03200"
     CDS             1975..2859
                     /locus_tag="VFQ35_03200"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9929662.1"
                     /translation="MSTVLVAGASGTLGRRVVSAAREQGFKVRALGRLRSSLESLGAD
                     EIALAEPVVGAGLDEAMAGVETVISALGASVSPEFGKGYRGFEAVDRPANLALIRAAE
                     RARVRRFVYVSMAGHREGAHLAYVRAHEAVVQALAASKLDHAVLRPTGFFAAFGAMVE
                     MAAKGPLPILGDPEVKTNPIDEADLAERCIELARQPELAGADIELGGPEIFSRRQIAE
                     LAFHVHGRPSKLRRLPNWVVSTMAFLMRPLNPRVSDLLRFVLSVSNRDCVAPSYGNRR
                     LEDYFARLVRTSPLRISG"
     gene            complement(2856..>3511)
                     /locus_tag="VFQ35_03205"
     CDS             complement(2856..>3511)
                     /locus_tag="VFQ35_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9929663.1"
                     /translation="FVERTIDRTLSRVHEEFPGIAVDAFCERAAWSLEDCRRLLERAA
                     SLGHPLRLHVDQFTRLGGLGLAKELGLRSVDHLEASDETELEAMAKQPIFGVMLPSTA
                     FHLRTPYPRARRFLDAGGRLVLASNVNPGSAPCSSLPFTVALAVRHCGVRVDEAIECI
                     TERAAELLGFADRGRIAVGQRADLVLLRHRDLRLLGMEFGGNPVDAVIVAGGLVSGG"
BASE COUNT          692 a         1099 c         1170 g          550 t
ORIGIN      
        1 agatggcggt cgagctgaag acgctcaaac gcggcaagca cgagagcatc ggcggcgtca
       61 cgttcgccgc ggctccaaaa cacttcgcgc tgaaggtgac gcgccaaaac gaggctctct
      121 acgacggcga cgtcgttccg cactactcgc cctatccgac caaaaaagaa gagaacaagc
      181 gcttctgcgg ggagcaagcc ttcgtgcagc cgaagtgcgt ccgcggcagc agcgagtgcg
      241 ctccgttccc gacgagctgc gacggcccgg aggactgcgg tgagcacgaa atctgctgtg
      301 gtagccccga gtcggcgcgc gattacggca aagatttcgc caccgagtgc tccgggaggc
      361 gacgctgctt ggagcgctac gggcacgtgg cttgccacaa ggattccgac tgccgctccg
      421 acgagacttg cgccgacgac ggcactgcgg ccgacttcaa gcccccgctg ctcgcatgtc
      481 gtccccgcgc cgagggcgcg cactaagggc tcgccgttat ggaatcctat cgggaggctg
      541 caaaggcagg cgcccggact ccattgcgct tcggctcggg attccctggt tttcgggcca
      601 cgcacgtcgt cgacgtcact cgcgcgggcg gtgtgatgaa tgatcgcgtc ctgtgaagcg
      661 cacacggtgt aatctccccg cgcggagaac aactcagaat gaccaaagcg gtatccctct
      721 cgctcgccgc gctcttgagc gtcggctgcg tgttcaacag cgtggcaaaa ctggatccca
      781 aggccgaaag cgtcgtcgtc gtgcacgaga cggacaagcc gaccgactgc aagtacctcg
      841 gcaagatcgt cggtcgctcg cacgccgaca atgaaaaaca ggccaagcaa ggcgcggaga
      901 acgatcttcg caatcaggct gccaagctaa aggctaattt cgccttgatc gagacccagc
      961 gtggcgggcc cgttggaacg acgagccaac gagaggtcgt gatgaacggc aaggcgctct
     1021 tctgcaagac gctcgagatg caacaggcgg aagaggccaa gcacgagcaa gagatgaaag
     1081 aaaaggaaga tcgcgagatt cgcgagcaag ccgagaagga gcgcaaggca gaagaagaga
     1141 aggaaaagaa agagcgagaa gagcaggagc gcaaagacaa gaagagcaag gactgaccgc
     1201 tgctcggcgc tcccggcttg gcgctgaatc gacgggcgag gaccgagggt ttctcgcccg
     1261 tgcgccgacg ttctcgcgtt gggagcgagc cgttactccg ggtgagtcag gtaggcggtc
     1321 agcagtgaca ccacttcgtc tcggtaacga ccttcgtcga cgtccgcggg acgcgggaac
     1381 agcccgcggt gagtgagact gaaagccgcg ttacatacga cgtgaagcgc gagttcgaga
     1441 tccgagcgac gaatctcggc gcgaaatgcc gagaggagct caccgagccc tcgagccgct
     1501 tgccgcgcct cttcgaccag cgcgggaaac tgcggaacac ggtcgagctc tcgcaatagc
     1561 gcgtcctgca gcgccggatc gcttgcccga aattcgaacg ccgcgtcgat cgccactcgc
     1621 agcgccgaac ggaggtcgtg cgcgcctgcg gccgccatgc gacccagaac gaagttggct
     1681 tcggcgcgcg cctgcctgcg caggagggct gccactagcg cctccttgtt gggaaaatac
     1741 tggtacaggg agccgacgct cacccccgcg cgagccgcga ttgcgttggt cgtcgcgctc
     1801 tctggagcgt ccgcggcctc gaccaaaatg cgagcgaggc catcgagcaa ggcgtctacc
     1861 gtgctctgcg atcgctcctg attcggcact ttccggggcc gcattgccgg cggtacgcca
     1921 gcgtcggaat gcgagttgtc cttggacatg ctgaaccggc aagatagccg agccatgagc
     1981 acggtgttag tcgcgggggc gagtggaaca ctcggtcgac gggtcgttag cgctgcccga
     2041 gagcagggct tcaaagtgcg cgctttgggc cgactcagga gctcgctcga gtcgctgggc
     2101 gcagatgaga tcgcgctcgc cgagccagtg gtcggcgcgg gcctcgacga agcgatggcg
     2161 ggggtagaaa ccgtaatttc ggcgctgggg gcgagcgtct cgcccgaatt cggaaagggg
     2221 taccgaggct tcgaagcggt agacaggccc gcgaacctcg cgttgattcg agccgcggag
     2281 cgagctcggg tacggcgctt cgtctacgtt tctatggcag gccaccgaga aggagcccac
     2341 ctcgcctacg tgcgagcgca cgaagcggtc gtccaggcgc tcgccgcgtc caagctggac
     2401 cacgcggtgc tgcgacccac cggatttttc gccgcgttcg gcgcaatggt ggagatggct
     2461 gcaaaggggc cgctacccat cctgggcgac ccagaggtca agacgaaccc catcgacgag
     2521 gcggatttgg cagagcgctg catcgagctc gctcgccagc cggagctcgc gggggccgac
     2581 atcgagctcg gcgggccgga gatcttcagc cgtcggcaga ttgcggagct cgcctttcac
     2641 gtgcacggcc ggccttcgaa gcttcggcga ctgccgaact gggtcgtctc gacgatggcg
     2701 ttcctgatgc gcccgctgaa cccgcgcgtg tcggatctct tgcgattcgt gctctccgtt
     2761 tcgaaccggg actgcgtggc tccgagttac ggcaatcgac ggctcgagga ctacttcgct
     2821 cgcctcgtcc gaacctcgcc gctgcgaatt agcggttagc ctcccgagac gagccctccc
     2881 gcgacgatga cggcgtccac tgggtttcct ccgaattcca tgccgagtag gcgtaagtcg
     2941 cgatgccgta gcaggactag atcggcgcgc tgtccgactg cgatgcggcc tcgatccgcg
     3001 aagcccagta gctccgccgc tcgctcggtg atgcactcaa tcgcttcgtc gacgcgaacc
     3061 ccgcagtgtc ggaccgcgag cgcgaccgtg aacggcagcg agctgcacgg cgcggagccg
     3121 gggtttacgt tgctcgccaa aacgagccgg ccgcccgcgt cgaggaagcg ccgcgcccgc
     3181 gggtacggcg tccggaggtg aaacgcggtg gatggcaaca tcacgccgaa gatcggctgc
     3241 tttgccatgg cttcgagctc ggtctcatcg ctcgcctcca aatggtcgac gctgcgaagg
     3301 ccgagctcct tcgcgagacc gagaccgccc aagcgcgtga actgatcgac gtgcagacgc
     3361 aagggatgtc cgagcgaagc ggcgcgctcg agcaggcgac gacaatcctc gagagaccag
     3421 gcggcgcgct cacagaaggc atcgaccgca atcccaggga actcctcatg aacgcgcgac
     3481 aacgtgcggt cgatggttcg ttcgacgaag t
//
LOCUS       DASTKS010000151         7781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_245396, whole genome shotgun sequence.
ACCESSION   DASTKS010000151 DASTKS010000000
VERSION     DASTKS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245396"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFQ35_03210"
     CDS             <1..1170
                     /locus_tag="VFQ35_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929664.1"
                     /translation="FLANMSHELRTPLNSLLILSQVLAENRDQNLTPEQVKFAQTVYT
                     SGHELLSLINEILDLSKVEAGKMPIVPKSFVLGDLREYLEQNFRHVAAQRALSFDIKM
                     DEGLPTTMFTDLGRLQQILKNLLSNAFKFTSKGGVTVRISSRQDERGTRGVAFAVQDS
                     GIGIPSDKQRLIFEAFQQADGTTSRKYGGTGLGLTISREIAKLLGGSIEVRSTPGEGS
                     TFTLTVPEAYSGQEAIQRDDSATGEDRDVPPLPEDVDFAGTKVLLVDDDLRNIFAIRT
                     VLEARGIQVLHAGNGRLALEMLLKHPEVNLVLMDTMMPELDGLTATRKIREDLALSKL
                     PIISLTAKAMKGDREKAIEAGASDYVTKPVDPKKLLSVIHQWRERSKLEEARTES"
     gene            1167..3665
                     /locus_tag="VFQ35_03215"
     CDS             1167..3665
                     /locus_tag="VFQ35_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929665.1"
                     /translation="MSDIQELTDTAPVLLVDDTAANLLALQAVLSPLGVATVAVRSGR
                     EALAEVEQRAFAVALVDVQMPEMDGFQLTRRLRETVNGRELPVLFVTALHRDEEYVRR
                     GYASGGADYITKPYDPQVIRARVKAFIDLYRQRDSLRREQVHQRTVERDEAIRRLMAL
                     ERISTAALETNDLGALLNELLATFIEAADAADSATILLRDGEWLTVVASVGSEHESSE
                     KVRVGEGFSGLIAGERKPLEVNRESISSVIQSPVLEGGARALYGVPLLHEGQVLGVAY
                     IGSTRAGQFSSAEKRLFLAAAERAALAVGRQLTASRLNEVLTSAPAFIAIASCLTGEF
                     VFVNPLMRELCAGPPGVAHPSRFGPEALKAMREVETSGKTVEIAQLRLPQTSDTEAPM
                     YVRFTAQPLRNLSGAVDRVLVFAVDITPQVNAHQELEEGQAVRARLLERERAARRAAE
                     LASAAKDEFLATVSHELRTPLNAILGWASIARAQPQSEVDRALAVIERNARAQARIVE
                     DVLDFSRMAKGKMRLSLQPVDLAEIIGSVLESVRPAAEAKGIRIQVDLKLARPLLADP
                     ERLQQVVWNLLSNAVKYCGASGEVTIHASSGDDAVLLSVADTGQGIDPAFLPHVFEPF
                     RQADGSTTRRHGGLGLGLAIVRQIVHAHGGTIEALSEGAGKGATFKVLLPIHASQSMA
                     LDDARAATDRASAPPSVRLDGVRVLLVDDDEDGREVMAQALIAHGADVTLVASAKKAL
                     AELRRARPHVLVSDIAMPDIDGYELIRRVRELPAVDGGATPALAVTAHAGKDVPARAL
                     ASGYHRYAAKPMDIESFISTVAALARDGAEARAS"
     gene            3795..4253
                     /locus_tag="VFQ35_03220"
     CDS             3795..4253
                     /locus_tag="VFQ35_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929666.1"
                     /translation="MSTEHQRDTEEGDAVFVVIEKGVGAPLALDHGKQAAEQTHVLSR
                     IDEEGGVEFARRVIGRLRRVRRAGLKIRSVAYVVPDTLPASAQRKNRLLSTLVRALEP
                     GARVTLIASYLSAGSALGWSQSLRAAARPGVTCDIVWQPSQQIDSDRTQV"
     gene            4371..5519
                     /locus_tag="VFQ35_03225"
     CDS             4371..5519
                     /locus_tag="VFQ35_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929667.1"
                     /translation="MQSSPVGKILVVDDSPLNLAAFSALLEPLGARVVCATSAAEAMP
                     LATGSDLALILMDVRMPDVDGYEAARLLREREGARDVPLIFVTAAEDEGLHAFRGYSA
                     GAVDFIVKPVDPLILRRKASVFLGLHRKSREVRDRAERMLEETRARDAVLGKLCHDFR
                     TPLNAIVGWAHLIRKGELDSVQVERALQSIHRNARGLTKLVVELGDVAGVSPGTAETL
                     SELTPISTSWRSQDSDPKAPSEGAKEDRESDGDPRPILTGIHALVVEDDPDGSDLIEA
                     VLKGFGASVTTAGTARDAWSALSQHPPDIIVSDLGLPDEDGFSLMRRIRRHALLSDLP
                     AIALSAYTSKRDVTEALAAGFQAHLAKPVEPRLLGDTIVRVSRREMQQ"
     gene            5614..6006
                     /locus_tag="VFQ35_03230"
     CDS             5614..6006
                     /locus_tag="VFQ35_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929668.1"
                     /translation="MSSEGAVPALPRVLVVEDDDDSREMLMELIASLGCTSIGAANAD
                     EASEHGDNGGWDLALIDLGLPDVDGCEVARRLRARDSGRRRKLVALTGFSDNATRAAA
                     REAGFDDFLVKPVFPEKLAQLLGASARS"
     gene            complement(6008..6514)
                     /locus_tag="VFQ35_03235"
     CDS             complement(6008..6514)
                     /locus_tag="VFQ35_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929669.1"
                     /translation="MIKRCSPKVFAMSALLALVGYAQGARADEVVVTRERSTVTGPNR
                     ALLHSGVWTLGLSYVPALVVAISSSESYDKKLYIPVAGPWMDFATRDCKYNCSHETVN
                     KALLVTDGIFQGIGALQLIGSFLFPETRSAVVASGKERRVAKRSNFQISPSRLGTDAY
                     GLTAFGRF"
     gene            6953..7117
                     /locus_tag="VFQ35_03240"
     CDS             6953..7117
                     /locus_tag="VFQ35_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET9929670.1"
                     /translation="MLYWAVVLFVIALIAAFLGFGGIAASAAGMAKILFFVFLVLAVV
                     ALIFGRRIPT"
     gene            7148..7519
                     /locus_tag="VFQ35_03245"
     CDS             7148..7519
                     /locus_tag="VFQ35_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9929671.1"
                     /translation="MNTKQTLVSLGMALASTKIAKRISNLEADDFWSLVGLERRRSHA
                     LSAVGLIGLGAVVGAGVALLFAPDSGTETRKRIMRQADKLGQAAGEFARDVQSEAPQL
                     LNRVANRGHENSVGEHSHQHS"
BASE COUNT         1361 a         2452 c         2586 g         1382 t
ORIGIN      
        1 ttcttggcga acatgtcgca cgagcttcgg acgccgttga acagcttgct catcttgtcg
       61 caagtgctcg ccgaaaatcg cgatcagaac ctgacccccg agcaggtgaa gttcgcgcaa
      121 acggtgtaca cgtccggcca cgagctgctc tcgctcatca acgagatctt ggatctctcc
      181 aaggtggaag ccggcaagat gccgatcgtg ccgaagagct tcgtgctcgg cgatctgcgc
      241 gagtacctcg agcaaaactt ccggcacgtc gccgcgcagc gcgcgctcag cttcgacatc
      301 aagatggacg aaggtctgcc gacgaccatg ttcacggatc tcgggcgctt gcagcagatt
      361 ttgaagaacc tgctctcgaa cgccttcaag ttcacctcga agggcggtgt gacggtccgc
      421 atctcgtctc gccaggacga gcgcggtacg cgcggcgtcg cgttcgcggt gcaggacagc
      481 ggcatcggca tcccgagcga caagcagcgt ctcatcttcg aggcattcca gcaggcagac
      541 ggcaccacga gccgaaagta cggaggcaca ggcctcgggc tcacgatcag ccgcgagatc
      601 gcgaagctgc tcggaggcag catcgaggtg cgcagcacgc ccggcgaggg cagcaccttc
      661 accctcaccg tccccgaggc gtactcgggg caggaggcga ttcagaggga cgactcggcg
      721 accggcgaag atcgcgacgt tcctccactg cccgaagacg tggattttgc gggcacgaag
      781 gttctcctcg tggacgacga cctacgcaac atttttgcaa tccgcaccgt gctcgaggcg
      841 cgcggcattc aagtgctgca cgccggcaac ggccgtttgg cgctcgagat gctgctgaag
      901 caccccgagg tgaacctggt gttgatggac accatgatgc cggagctcga cggtctcacc
      961 gcgacccgaa agattcgtga agatttggcg ctatcgaagc tgcccatcat ctcgctcacc
     1021 gcgaaggcaa tgaagggcga tcgcgaaaaa gcgatcgagg caggcgcctc ggactatgtt
     1081 acgaagccgg tagatccgaa aaagctgctc tccgtcattc atcaatggcg tgagcgatcc
     1141 aagctcgagg aggcgcggac cgagtcgtga gcgacattca ggaactcact gatactgctc
     1201 cggtcttgtt ggtcgacgac acggccgcga acttgctcgc gctgcaggcg gtgttgagtc
     1261 cgctcggcgt cgcgacggtc gcggtgcgct cggggcgcga ggcgctggcg gaggtcgagc
     1321 agcgggcctt cgcggttgcg ctcgtcgacg tgcagatgcc cgagatggat gggttccagt
     1381 tgacgcgccg tctgcgagag accgtcaacg ggcgcgagtt gccggtgctg ttcgtgaccg
     1441 cgctgcatcg cgacgaggag tacgtacggc gcggctacgc gagcggcggg gccgactaca
     1501 tcacgaagcc ctacgatcca caggtcatac gggcccgcgt caaggcgttc atcgacctgt
     1561 atcgtcagcg tgacagcttg cgtcgcgagc aagttcacca gcgcacggtc gagcgcgacg
     1621 aagcgattcg gcgcctgatg gcgctcgagc gcatttcgac cgcagcgctc gagacgaacg
     1681 atctcggcgc gctcctgaac gagctcctcg ccactttcat cgaagcggcc gacgcggctg
     1741 attcggcgac tattttgttg cgcgacggcg agtggctcac ggtcgtcgcc tcggtaggct
     1801 ccgaacacga gtcgagcgag aaggtgaggg tgggggaggg cttctccggg ctcatcgcgg
     1861 gcgaacgcaa gccgctcgag gtgaaccgag agtcgatttc gagcgtgatt caaagcccgg
     1921 tgctcgaggg tggggcgcga gctctgtacg gcgtgccgct cctgcacgag ggtcaggttc
     1981 tgggcgttgc atacatcggc tcgactcggg ccggccagtt cagctccgcc gagaagcgac
     2041 tgttcctggc cgcggccgag cgcgcggcgc tcgcggtcgg gcgtcaactc acggcgagtc
     2101 gattgaacga agtgctgacc tcggcgcccg ccttcatcgc catcgcgagc tgcttgacgg
     2161 gtgagttcgt gttcgtgaat ccgctgatgc gcgagttgtg cgccggtcca cccggcgtcg
     2221 cgcatccgag ccgcttcggc cccgaagcct tgaaggcgat gcgcgaggtg gagacctctg
     2281 gcaagacggt cgaaatcgct cagctcaggc tgccgcagac cagcgacacg gaggcgccga
     2341 tgtacgtgcg cttcactgcc caaccgctgc ggaacttgag cggcgcggtg gatcgcgtgc
     2401 tcgtgttcgc ggtcgacatc accccccaag tcaacgcgca ccaagagctc gaagagggac
     2461 aggcggtccg cgcgcgcctg ctcgagcgtg aacgcgccgc tcggcgcgca gccgagctcg
     2521 cgagcgcggc caaggacgag tttttggcga ccgtttcgca cgagctgcgc accccgttga
     2581 acgccatctt gggatgggca tccatcgccc gagcccagcc ccagtccgag gtggaccgag
     2641 cgctcgcggt gattgagcgc aacgcgcgcg cccaagcgcg catcgtggaa gacgtgctcg
     2701 acttttcgcg catggcgaag ggcaagatgc ggctctcgct gcagcccgtc gatctggccg
     2761 agatcatcgg gtccgtcctc gagtcggtgc gccccgcggc ggaggccaaa gggattcgca
     2821 tccaggtcga tctgaaattg gcgaggccgc tgctcgcgga tccggagcgc ctgcaacaag
     2881 tcgtgtggaa cctgctctcg aacgccgtga agtactgcgg cgcgtccggc gaggtgacca
     2941 ttcacgcgag tagcggtgac gacgcggtgc tgctgagcgt cgcggatacc gggcagggca
     3001 tcgatcccgc ctttctgccg cacgtcttcg aaccgttccg ccaagccgac ggctccacga
     3061 cgcgccgcca cggcgggctt gggctggggc tagcgatcgt ccgccagatc gtgcacgcgc
     3121 atggcggcac gattgaggcg ctgagcgaag gcgcgggcaa gggcgcgacg ttcaaggtgc
     3181 tgttgccgat ccacgcgagc cagagcatgg cgctcgacga cgcgcgcgcg gcgacggatc
     3241 gcgcttccgc gccaccgagc gtgcggctgg acggcgttcg agtactactg gtcgacgatg
     3301 acgaggacgg ccgcgaggtg atggctcaag cgctcatcgc gcatggagcc gacgtcaccc
     3361 tggtcgcctc ggccaagaag gcgctcgccg agctgcggcg cgcgcggcct cacgtgctcg
     3421 tgagcgacat cgcgatgccc gacatcgacg gttacgagct catccggcgc gtacgcgagc
     3481 tccctgccgt cgacggcggc gcgacgcccg ccttggccgt caccgcgcac gccggcaaag
     3541 acgtgcccgc tcgtgcgctc gcttcggggt accatcgcta tgccgccaag ccgatggaca
     3601 tcgagagctt catctcgacg gtggcggccc tggcccgcga cggagccgag gcgcgcgcca
     3661 gctgaggaag cgcacgcgcc gcacctgagg cgacgctgct gctcggagcc tggagtgcgt
     3721 gtgcgaatgt cgcctccttt cagcgcgttc cctgggaatg aaggtggcac accgcgtgct
     3781 taggtggatg cagcatgtca accgagcacc aacgcgacac ggaagagggc gacgccgtgt
     3841 tcgtcgtgat cgagaagggc gtcggcgccc ctctggctct agatcacgga aagcaagcgg
     3901 cggaacagac gcacgtgctc agtcgaatcg acgaagaggg cggggtcgaa ttcgcgcggc
     3961 gcgtgatcgg gcgcctacga cgggtccgac gagctggtct caagattcgg agcgtggcct
     4021 acgtagtgcc cgatacgctc ccggcgagcg ctcaacgcaa gaaccgcctg ctcagcacgc
     4081 tggtccgtgc gctcgagcct ggagcccgcg tgaccctcat cgcttcctac ctcagcgccg
     4141 ggagcgcgct cggctggtcg cagtcgctac gcgccgccgc gaggcctggg gtgacctgtg
     4201 acattgtgtg gcagccaagc cagcaaatcg attccgaccg tacccaggtg taacaagttc
     4261 gccacggtgt ggcgatcatg tatgcgtaaa tcgccacgtt ggggacgtat ttcggctgaa
     4321 tgttgcgatc atgaaatcgg cgtggcgctt gcagagaaga ctacaggcgc atgcagagct
     4381 cgccagtcgg aaagatcctc gtcgtcgacg acagcccgct gaacttggcg gcgttctcgg
     4441 cgctgctcga gccgctcggg gctcgggtag tctgcgcgac ctctgcggcg gaggcgatgc
     4501 ccctggcgac cggctcggat cttgcgttga ttttgatgga tgtacgcatg cctgacgtcg
     4561 atggctacga ggcggcgcgc ctcttgcgcg agcgcgaggg cgctcgcgac gtgccgctga
     4621 tcttcgtgac cgctgcggag gatgagggct tgcacgcatt tcgaggttac tcggctggcg
     4681 cggtcgactt catcgtaaag cccgtcgatc cgctcatttt gcgccgcaag gcgtcggtct
     4741 tcttgggact gcaccgcaag tctcgcgaag tgcgcgaccg tgccgagcgc atgctcgagg
     4801 agacccgcgc gagagatgcg gttttgggga agctctgtca cgactttcga acgccgctca
     4861 acgctatcgt cgggtgggcg catctgatta ggaaaggcga gctggattcg gtccaagtcg
     4921 agcgggccct ccaatccatt catcgcaacg ctcgcggtct taccaagctc gtcgtcgagc
     4981 tgggggacgt cgcgggagtt tcgccgggaa ccgcggagac attgtcggag ctcacaccga
     5041 tctcgacgag ctggcgcagc caagattcgg atcccaaagc gccttccgaa ggagccaagg
     5101 aggatcggga atctgacgga gatccgcgtc ccatcttgac tggaatccac gcactcgtcg
     5161 tcgaggacga tccggacggt agcgacctga tcgaggccgt gttgaagggc ttcggcgcga
     5221 gcgtaacgac ggcgggcacc gcgcgcgacg cgtggagcgc gctctctcag cacccaccgg
     5281 acatcatcgt gtccgatttg gggcttccgg acgaggatgg cttctcgttg atgcgccgta
     5341 ttcggcggca cgcgctgctc tcggacctgc cggcaatcgc gctgagcgcg tacacctcca
     5401 agcgcgacgt gaccgaagcg ctcgcggctg gatttcaggc acacctcgcg aagccggtgg
     5461 agccgcgcct cctcggagat accatcgttc gggtatcgcg ccgcgaaatg cagcagtgat
     5521 cgcacgatca gctttgtacg gggcggccgt ttggtcagcc tcggagacgc gctctagcgc
     5581 cgccgaccca catgggtaaa cttcgtgctt cgaatgtctt ccgagggagc cgtaccagca
     5641 ctgccgcgtg ttctcgtcgt ggaagacgac gatgactcgc gcgagatgtt gatggaactc
     5701 atcgcgtcgc tcggctgtac gagcatcggc gcggcgaacg cggacgaagc gagcgagcac
     5761 ggcgacaacg gcggctggga tttggcgctc atcgatttgg gcctccccga cgtcgacggt
     5821 tgcgaggttg cgcggcggct tcgcgctaga gattcgggtc ggcgacgaaa gctcgtcgcg
     5881 ctgactggct tttccgacaa cgcgactcgc gccgccgctc gagaggccgg gttcgacgac
     5941 ttcttggtca agcccgtgtt ccccgagaag ctcgcgcagc tgctcggggc ttctgcacgg
     6001 agctgagtta gaacctaccg aacgcagtga gcccgtaggc gtcggtgccg agccgcgacg
     6061 gcgaaatttg aaagttcgaa cgcttcgcga cgcgccgctc cttgccgctc gcgacgaccg
     6121 cgctcctcgt ttcagggaac aaaaaggagc cgatgagttg cagcgcgcca atgccctgga
     6181 agatgccgtc cgtgacgagc agcgccttgt tcactgtctc gtggctgcag ttgtacttgc
     6241 agtcgcgggt cgcgaagtcc atccacggcc ccgcgacggg aatgtagagc tttttgtcgt
     6301 aagactccga gctgctgatc gcgacgacta gcgcgggcac gtagctgagc ccgagcgtcc
     6361 aaacgccaga gtgcagcagt gcgcggttcg gccccgtcac agtgctgcgt tcgcgcgtaa
     6421 cgacgacttc gtcagcgcgt gcgccctgcg cgtagcccac caaggcgagc aacgcgctca
     6481 tcgcgaatac ttttggtgag caacgtttga tcatgacttc ctcgtttgca cttggaacta
     6541 cgctggctcc gcacccgccg atcgggctcg attcccggat atcttcgagt tgtcggttcg
     6601 gtgcgtgcgg agcacagcgt tcgttccaat caaccctcta cgcaaaggga gtgccaagcg
     6661 cggtgaggac ggagtgcgcc acgcgactcc agaattcgcg gagaatgtcc gtcgagatct
     6721 cggttctgct cgctcgggtg catagtcgcc tccgaggcgc agcattgcca caaagaggca
     6781 cgcgcgcccg caccgaaaac gccttcgacg cggtcgtgcc gggtcgctat taacgagtca
     6841 gggccgtgtg tccgcgggga tgagttggct gttcgcgtct ggcacgcctt tcgctctaga
     6901 cgactacagc gtcacgaaga cgcaactcgc ataacgcaat tggagtagcg ccatgttgta
     6961 ttgggcagtc gtattgttcg taatcgcgct catcgcggcc ttcttgggct tcgggggcat
     7021 cgccgccagc gccgctggca tggccaagat cctgttcttc gtgttcctgg ttcttgcggt
     7081 ggtcgcgctc atcttcggcc gtcgcatccc gacctaacgc gtagaccgca acttcaggag
     7141 aatcgaaatg aacactaagc agactctggt ttctctgggt atggccctcg cttcgacgaa
     7201 gatcgccaag cgcatctcga accttgaagc ggacgacttc tggtcactcg tcggcctcga
     7261 gcgccgccgc agtcatgcgc tgagcgcagt tggcctgatt ggcctgggcg ccgtcgtcgg
     7321 cgctggcgtc gcgctcctgt tcgctcccga tagtgggacc gaaactcgca agcgcatcat
     7381 gcgtcaagcg gacaagctcg gtcaggcggc gggcgagttc gcgcgcgacg tgcagtccga
     7441 ggcgccgcag ctcttgaacc gcgtcgccaa ccgcgggcac gagaacagcg tcggcgagca
     7501 ctcgcatcag cattcctgag ccgccgagcg gctcggtttc gtcttctcgc gggcgcggtc
     7561 gcgtgtgttt ccgcacgtcg cgaccgccgc atgcgaagac gttcggtaaa ctggttgact
     7621 tatgtcgctt tcgagcgagc cttttcttag ttcgcaaccg ggcggggcac acaacgcccc
     7681 gccatgtgcg cccaccgtcg agaggcgaaa cgccgccgtt accgcaggcc gccctgccgc
     7741 gcacggaacc gtgcaagaac gcctcgttgc gttttcacaa t
//
LOCUS       DASTKS010000152         6321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1808220, whole genome shotgun sequence.
ACCESSION   DASTKS010000152 DASTKS010000000
VERSION     DASTKS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808220"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFQ35_03250"
     CDS             complement(<1..328)
                     /locus_tag="VFQ35_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929672.1"
                     /translation="MHSMTLMRTLSVCVLSVWLAACASSQSPATAQVRRPNIPEVYTA
                     NGQVLGVERPLPEDAVTNVHLVFQARGEEPVRVELGPGWYLDEGKLHFDPKDDILVEG
                     RREVHNG"
     gene            531..992
                     /locus_tag="VFQ35_03255"
     CDS             531..992
                     /locus_tag="VFQ35_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9929673.1"
                     /translation="MKVRELMSHNVCTCSPETSLHEAAHIMWTCDVGCLVVTDDDRRP
                     IGMITDRDITMAAYTQGVTLHDARVSSAMARTIVTCSVDASSHEVELLMNRAQVRRVP
                     VVDALGRLMGIVALADLARAAQHPLHIAEMPGLTKTLAAITQRRLDGMTGI"
     gene            complement(989..1168)
                     /gene="ccoS"
                     /locus_tag="VFQ35_03260"
     CDS             complement(989..1168)
                     /gene="ccoS"
                     /locus_tag="VFQ35_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HET9929674.1"
                     /translation="MTILFLVLPLTIGISAISVAAFAWATRKGQFDDLETPALRILHD
                     DTPRSTPTRSPSEGA"
     gene            complement(1165..3630)
                     /locus_tag="VFQ35_03265"
     CDS             complement(1165..3630)
                     /locus_tag="VFQ35_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9929675.1"
                     /translation="MTISAFPTTPELVASADVACSHCGLPVPSARVESAARFQFCCDG
                     CRSVHTILNTTGLESYYALRGAQGTAPARVSQRRYAEFDDAAFIERHVRSPAPNVSQS
                     ELFLEGIHCSACVWLVERLPRVVPGVVEARLDLGRSELSVVWERDRVKFSEIARGLAS
                     LGYPPHASAASQAEAERRATDRTLLLRIGIAGAAAGNVMLMALALYSGLFSHMDAEYA
                     ELFRWGSFAIATPTVAWTGNVFFRGAWASLRTKTPHMDLPITIGILAGYVGGAVNTLR
                     GSGEVYFDSLCTLIFLLLVGRWLQRKHHRRAANASDLLTALAPSVAEIEFEGERREVP
                     AESVTTGSIVVVRAGERIPVDGVVTLGSSSVDSALLTGETAPVSVSIGDRVYAGTINQ
                     ASEIRLRCESSGEETRVGRLMQSVEQAQRERAPIVRLADRVAGYFVWVILTLAAVTLV
                     IWAHVDPAHAVDHTVALLVVTCPCALGMATPLAVSAALSRAARQGLLFKGGEFVEELA
                     RPKLVVFDKTGTLTEGRLELVARSGDERAFELARAAEAQSNHPIARAFQRALPELPGS
                     IAEGMTETAGGGLVAEVSGHAVCVGSLGFVEPRVTSVPEWAREFTDAQAALGRTPVLV
                     AIDGELRAAAAFGDRLRADAATALTRLRKLGVQVALLSGDHQRVVDAVVGELGGAWLL
                     ASGGTSPEQKLARVREYCRAQRGVVMVGDGVNDAAALSSANVGIALHGGAEASLAAAD
                     VFTTEPGLMRVVEAVEGSRRTLRVIQRGVAISLAYNLVGVGLAVAGVLSPLWAAVLMP
                     LSSVSVVTLALRSRTFAPERASS"
     gene            complement(3627..4349)
                     /locus_tag="VFQ35_03270"
     CDS             complement(3627..4349)
                     /locus_tag="VFQ35_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9929676.1"
                     /translation="MTGAVLLSIIAASALGSAHCAAMCGGFVVAYAGSDANGVERRVH
                     LAYHGGRLVAYTALGALAGLLGRAIDLAGAAAGYATAAAVVAGSLMVLWGLAAMLESQ
                     GVRLPKLNLGALAGHSARLIGGLRVHSRGARALSLGLCSALLPCGWLYAFVVSAAGTA
                     SPLWGALLMGAFWSGTVPALLGMGVLAARFARPFRRHLPFLSACVILCVGLFTVVSRA
                     NMPSFAAPIDAKHKPACACHHR"
     gene            complement(4352..4846)
                     /locus_tag="VFQ35_03275"
     CDS             complement(4352..4846)
                     /locus_tag="VFQ35_03275"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET9929677.1"
                     /translation="MNTQTLRDNRWAFVPVGLLAAMFVGLGWMARVATNDPGFALERD
                     YYAKAVRWDQHRAEEENDARLGYRLELSSVSSLSSEHKAEVTLRLVDAYGGALRGAHL
                     NAEAFAVARSADVRALRFQEISPGTYRTELDRARAGIWELRCVAARTGERFTHTLRLE
                     LIEK"
     gene            complement(4843..6231)
                     /gene="ccoG"
                     /locus_tag="VFQ35_03280"
     CDS             complement(4843..6231)
                     /gene="ccoG"
                     /locus_tag="VFQ35_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HET9929678.1"
                     /translation="MSAPISPERVLPTLNADGSRFLIRPRLYPGRFHRRRRWVAWALM
                     ALFIGLPFVKVGGKPAMLLDVAERHFTFFGHTFLATDGVLLMFFMLSVFATVFFVTAL
                     VGRGFCGWACPQTVYMEFLFRPIERLFEGKREDQLRIDKNGFSYRRLAKNGVFLVLAF
                     ALGNIFLSYFVGVDRLVHWMSHSPVEHWGGFLVMAVTTGLVFFDFSYFREQMCTVICP
                     YARLQSVLLDRRSLVVGYDKKRGEPRSKGKPKPGNGDCIDCSACVVACPTGIDIRKGL
                     QLECIACAQCVDACDSIMDKIKKPRGLIRYGSQNSLDHAGKTKILRTRVFIYAGLLVV
                     LFSALALTGRAKQKTDVTVLRGLAAPFTLSGDEVTNLLRLKIQNRDPVAHHYSLTLLD
                     APGAKVIAPELPLEVPPMEQRSTSFFVIEKTSAFVNGERRVRLRVRDEAGFSSDSAYK
                     LLGPTNTSAPSP"
BASE COUNT         1139 a         2042 c         2172 g          968 t
ORIGIN      
        1 tcccgttgtg cacttcgcgg cggccttcga ccagaatatc gtccttcgga tcgaagtgga
       61 gcttgccctc gtccaggtac cagcccggac cgagctcgac ccgaaccggc tcttcgccac
      121 gcgcctgaaa caccaagtgc acgttcgtca ccgcgtcctc gggtagtggc ctctcgacgc
      181 cgaggacctg gccgttagcc gtgtacacct cggggatgtt ggggcgacga acctgtgccg
      241 tcgctggcga ctgcgacgac gcgcaagccg ctagccaaac cgagagaacg catacggaaa
      301 gcgtgcgcat gagcgtcatt gagtgcatgg ctcgtaccaa taccttcaag gtgcagggat
      361 gggcgtttgt tccgcgggag cgagcgtttt cgccgcacca gtgaccattt ggagcgtgca
      421 ccttttgcat cggctgcata aaccagcgaa atggacgacc tcggcgttgg actagggttt
      481 gcagctcggg gcgcgctcgt cggagtgcga gcctgcgtgg aggttcgacc atgaaagtgc
      541 gagagttgat gtcccacaac gtttgcacgt gctcgccgga gacgtcactg cacgaggcgg
      601 cgcacatcat gtggacgtgc gacgtcggct gcctcgtcgt caccgacgac gatcgcaggc
      661 ccattggaat gatcacggat cgcgacatca cgatggcggc gtacacccaa ggtgtcactc
      721 tgcacgatgc gcgcgtgagc agcgcgatgg cgaggaccat cgttacgtgt tcagtcgatg
      781 cctcgtcgca cgaggtggag ctcttgatga accgtgctca ggtccggcgg gttccggtcg
      841 tcgacgcgct cggacgcctg atgggcatag tagccctggc ggatttggcg cgagcggcac
      901 agcatcccct ccacatcgcg gaaatgcctg ggctcaccaa gacgctcgcc gcaatcactc
      961 agcgccgctt ggacggcatg accgggatct aggcgccctc gctcggcgat ctcgtcggag
     1021 tcgaacgtgg cgtgtcatcg tgcaggatcc gcaaggcggg cgtttcgaga tcgtcgaact
     1081 gtccctttcg ggtcgcccag gcgaatgccg ccacggaaat ggccgagatc ccgatcgtga
     1141 ggggaagcac gagaaatagg atggtcatga cgaggctctt tccggggcga acgttcggga
     1201 gcggagcgcg agcgtgacta cgctgactga gctcaacggc atcagcaccg ccgcccaaag
     1261 ggggctcaag actcccgcta cggcgagccc caccccgacc aggttgtacg cgagtgaaat
     1321 ggcgacgccg cgctggatca cgcgcagcgt gcggcgggac ccctcgaccg cctcgacgac
     1381 ccgcatcaag ccgggttcgg tggtaaaaac gtcggccgcg gcgaggctcg cctcggctcc
     1441 gccgtggagt gcaatgccga cgttcgccga agaaagcgcg gcggcgtcgt tcacgccgtc
     1501 tccgaccatg accacgccgc gctgcgcccg gcagtattcg cgcacgcgcg cgagcttctg
     1561 ctcggggctg gtgccgccgc tcgccaagag ccacgcgccg ccgagctcgc cgacgaccgc
     1621 gtcaacgacc cgctgatggt ctcctgacaa gagcgccacc tgaacgccga gcttgcgcag
     1681 gcgggtgagg gccgtggccg catcggcgcg caatcggtcg ccaaacgccg ccgccgctct
     1741 caactcgccg tcgatggcga cgagcacagg ggtgcgcccc agcgcggctt gcgcatccgt
     1801 gaactcgcga gcccactccg gcacgctggt gacgcgcggt tcgacgaagc cgagcgaacc
     1861 gacgcaaacg gcgtggccgc tgacctccgc gacgagcccg cctcccgcag tctcggtcat
     1921 cccttcggcg atcgagccag gaagctcggg caaggcgcgc tggaacgcgc gtgcgatcgg
     1981 gtggttcgat tgtgcctcgg ccgcgcgggc cagctcgaac gcgcgctcat cgccgctccg
     2041 cgcgaccagc tcgagtcgcc cctccgtcag cgtgcccgtt ttgtcgaaga cgacgagctt
     2101 cggacgcgcg agctcttcga cgaactcgcc gcccttgaac aagaggccct gtcgagcggc
     2161 gcgcgagagc gccgcactca ccgcgagcgg cgtggccatg ccgagcgcgc acgggcacgt
     2221 gacgaccagt agcgcgacgg tgtggtctac cgcgtgagct ggatcgacgt gcgcccagat
     2281 caccaacgtg acggcggcga gcgtgaggat tacccaaacg aagtagccgg cgacccggtc
     2341 ggcgagccgc acgatgggcg cgcgttcgcg ctgcgcttgc tcgacgctct gcatgaggcg
     2401 accgacgcgc gtctcttccc cggagctctc gcaacgcagg cgtatctcgc tcgcctggtt
     2461 gatggtgccg gcgtagacgc ggtcgccgat cgagacgctg accggcgcgg tctcaccggt
     2521 gaggagcgcc gaatcgacgc tgctcgaccc gagcgtgacc acgccgtcga cggggatgcg
     2581 ttcgcccgca cgtacgacga cgatggagcc ggtcgtgacg ctttcggccg gcacctcacg
     2641 gcgctcgcct tcgaactcga tttcggcaac cgacggggcg agagcggtga gcaggtccga
     2701 ggcgttcgcg gcgcggcggt ggtgctttcg ttggagccag cgcccgacga gcaggaggaa
     2761 aatgagcgta cagagcgagt cgaagtagac ttcgccgctg ccgcgcagcg tgttcaccgc
     2821 accgccgacg tagccggcga ggatcccgat cgtgatcggc aaatccatgt gcggcgtctt
     2881 ggtgcgcagt gaagcccacg cgccgcggaa aaacacgttc ccggtccaag cgacggtcgg
     2941 ggtcgcgatc gcgaagcttc cccaccggaa gagctccgcg tactccgcat ccatgtgcga
     3001 gaagagcccg ctgtatagcg cgagcgccat cagcatcacg ttgcccgcgg ctgcaccagc
     3061 gatgccgatg cgcaacagca acgtgcgatc cgtagcgcga cgctcggcct cggcctggct
     3121 cgccgccgat gcgtgcggcg ggtagccgag agaggcgagg ccgcgagcga tttcgctgaa
     3181 cttcacgcgg tcgcgctccc agaccaccga cagctcggag cggccgaggt cgaggcgcgc
     3241 ctcgacgacg ccggggacga ctcgcggcaa gcgctcgaca agccagacgc aggcgctgca
     3301 gtggatgccc tccaagaaga gctcgctttg gctcacgttc ggggcggggc ttctcacgtg
     3361 ccgctcgatg aacgcggcgt cgtcgaactc ggcgtagcgg cgctggctca cgcgggctgg
     3421 ggccgtgcct tgagcgccac gcagggcgta atagctctcg agcccggtgg tgttcaaaat
     3481 cgtgtgcacc gaacggcagc cgtcgcagca gaattgaaag cgcgcagcgc tctcgacgcg
     3541 tgcgctcggc acggggagcc cgcagtgcga acaggcgacg tctgcggaag cgacgagctc
     3601 aggcgtggtt ggaaaagcgc ttatcgtcat cgatggtggc acgcacaggc cggcttgtgc
     3661 tttgcgtcga tcggtgcggc gaacgacggc atgttggcgc gcgacacgac cgtgaagagg
     3721 cccacacaca agatgacgca ggcgctcaag aaggggaggt ggcgacggaa tggccgtgcg
     3781 aagcgggcgg cgagcacgcc cattccgagc agcgccggta cggtgccgga ccagaacgcc
     3841 cccatgagca gtgcgcccca gagcgggctc gcggtgccgg cagcggagac gacgaaagca
     3901 tacagccagc cgcacggaag cagcgcggaa cacagcccca aggacaacgc gcgcgccccg
     3961 cgggagtgga cgcggagccc gccgatcaag cgggcgctgt ggcccgcgag cgcgccgaga
     4021 ttcagcttgg ggagccttac accttggctc tctagcatcg ccgcgagccc ccacagcacc
     4081 atcaacgagc ccgcgacgac tgcggcggcg gtcgcgtagc ccgcggcggc tcccgcgaga
     4141 tcgatggcgc ggccgagcaa gccggcgagc gcgccgagcg ccgtgtaggc gacgagtcgt
     4201 cccccgtgat aggcgaggtg aactcgacgc tcgaccccgt tcgcgtccga acccgcgtac
     4261 gctaccacga agccgccgca catggccgcg cagtgggcgc taccgagcgc gctcgcagcg
     4321 atgatcgaaa gtagcacggc gcccgtcatg actacttctc gatgagttcg aggcgcaggg
     4381 tgtgcgtgaa acgctcgccg gtgcgcgcgg cgacgcagcg gagctcccaa atcccggcgc
     4441 gcgcccggtc gagctcggtt cggtaagttc ccggggagat ctcctggaag cggagcgcgc
     4501 gaacatctgc cgagcgagcg accgcgaagg cttccgcgtt caggtgagcg ccacgcagcg
     4561 ctccgccgta agcgtcgacc aagcggagcg tgacctcggc cttgtgctcg ctcgagagcg
     4621 agctcaccga cgagagctcg agccggtacc cgagccgagc atcgttctcc tcttcggcgc
     4681 ggtgctggtc ccagcgcacc gctttcgcgt agtagtcacg ctcgagtgcg aaccccgggt
     4741 cgttggtggc gacccgcgcc atccagccga gcccgacgaa cattgcagcg agtagtccta
     4801 cgggaacgaa agcccagcgg ttgtcgcgga gggtttgagt gttcatggag acggggcgct
     4861 cgtgttggtt gggccgagca gcttgtaggc agaatcagag ctgaagcctg cttcatcgcg
     4921 cactcgcagg cgaacgcggc gctcgccgtt gacgaaggcg gacgtcttct cgatgacgaa
     4981 gaacgacgtg gagcgctgct ccatgggcgg cacctcgagc gggagctcgg gcgcgatcac
     5041 cttggcgccg ggcgcgtcga ggagagtgag gctgtagtga tgcgcgaccg gatctctgtt
     5101 ttggatcttg aggcgcagga ggttcgtgac ttcgtcgccg ctcagggtga acggagcggc
     5161 gaggccgcgg agcaccgtca cgtctgtctt ttgctttgcg cgaccggtga gggcgagcgc
     5221 gctgaacaag actacgagca ggcccgcgta gatgaacacg cgcgtcctca gaatcttcgt
     5281 cttgccggcg tggtcgagcg agttttgcga gccgtagcga atgagcccgc gcggcttctt
     5341 gatcttgtcc atgatcgagt cgcaggcgtc cacgcactgc gcgcacgcga tgcactcgag
     5401 ctggagtcct ttgcggatgt cgatgccggt cggacacgcg accacacagg cgctgcagtc
     5461 gatgcagtcg ccgttgccgg gcttcggctt ccccttgctg cgcggctcgc cgcgcttctt
     5521 gtcgtagccg acgacgaggg agcgccggtc gagcaacacc gactggagcc gcgcgtaggg
     5581 gcagatgacg gtgcacatct gctcgcgaaa gtacgaaaag tcgaagaaca cgagccccgt
     5641 cgtcacggcc atgacgagga acccccccca gtgctcgacg ggcgagtggc tcatccagtg
     5701 aacgagccga tcgactccga cgaaatacga aaggaagatg tttcccagcg cgaacgcgag
     5761 aaccaggaag accccgttct tcgccaggcg acggtagctg aacccgttct tgtcgatgcg
     5821 cagctggtct tcgcgcttac cttcgaacag ccgctcgatt gggcggaaca agaactccat
     5881 gtacacggtc tgcggacaag cccagccgca gaagcctcgc ccgacgagcg cggtcacgaa
     5941 gaagacggtc gcgaagacgc tcagcatgaa gaacatcagg agcaccccgt cggtcgcgag
     6001 gaacgtatga ccgaagaagg tgaagtgacg ctccgcgacg tcgagcagca tcgcgggctt
     6061 gccgccgacc ttgacgaagg gcaatccaat gaagagggcc atcagcgccc atgccaccca
     6121 gcgccggcgt cgatggaagc ggccggggta gagacgaggg cgaatcagaa accgcgagcc
     6181 gtccgcgttg agggtgggga ggactcgctc tggggaaatg ggcgctgaca tggaaggtgg
     6241 gtccttgcgc gtcattgaac gcgcgtgccc tccgcgggtt tacctgggac gttgctgttt
     6301 cgaatcgtgg tgatgaaagc t
//
LOCUS       DASTKS010000153         4529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1808452, whole genome shotgun sequence.
ACCESSION   DASTKS010000153 DASTKS010000000
VERSION     DASTKS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808452"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFQ35_03285"
     CDS             complement(<1..209)
                     /locus_tag="VFQ35_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HET9929679.1"
                     /translation="MKGRVDWFLVALLSVSLLAFVWPGPGSQGGALHPELANKLGVAL
                     IFLLHGASLSLAALRAGLLQVRVHLL"
     gene            complement(206..1681)
                     /locus_tag="VFQ35_03290"
     CDS             complement(206..1681)
                     /locus_tag="VFQ35_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET9929680.1"
                     /translation="MSKFSIADLMARSGVKFGTSGARGLVRDLTDRIAFAYTAGFLNH
                     LAQSGQLGGAHAVVVGGDLRPSTERIMSAVARAVHTRGLEVVHAGRVPSPAVMLHGLA
                     HRLPSIMVTGSHIPDDRNGIKFNTGLGEISKADEIGIRSQTVELPDLFDEEGQLRAGS
                     SSLPTADPEPARAYVQRWLRAFPRSLAGLRVGVYGHSAVGRELLVEILEQLGAEVTRL
                     GWSERFIPVDTEAIRAEDVKLAAEWAREHRLDALVSTDGDSDRPLIADESGTFLRGDV
                     ACVLAARYLGATFVAAPVSCNSVLEKSSWFSARRTRIGSPFVIEAMLEAKAGGESCVV
                     GYEANGGFLHVSELSVTGGGKLSPLPTRDPVVVQLALLTEAKRRGVPLSRLALDLPQR
                     FTASDRIENFAPELGQKLCAELASGGAAAAEKAFPALGRFKEKNELDGVRITFENDEV
                     VHVRPSGNAPELRCYVEASSVARGDELLRYAMARLAEVARR"
     gene            complement(1761..4468)
                     /gene="aceE"
                     /locus_tag="VFQ35_03295"
                     /pseudo
     CDS             complement(1761..4468)
                     /gene="aceE"
                     /locus_tag="VFQ35_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
BASE COUNT          760 a         1518 c         1476 g          775 t
ORIGIN      
        1 aggaggtgaa cgcgcacctg aaggagaccc gctcgcagcg ccgcgagcga aagactcgcg
       61 ccgtggagca agaagatgag cgcaactccg agcttgttgg caagctctgg atgcagcgcg
      121 ccgccctgac tccccggccc gggccacacg aacgcgagga gactgaccga gagcagcgcg
      181 acgaggaacc agtcgacgcg ccccttcatc gacgcgcgac ttcggcgagc cgcgccatgg
      241 cgtagcgcaa aagctcgtca ccgcgcgcca cgctgctcgc ctcgacgtaa cagcgaagct
      301 ccggcgcgtt ccccgacggg cgcacgtgca cgacttcgtc gttttcgaag gtgatgcgca
      361 cgccgtcgag ctcgttcttc tccttgaagc gaccgagcgc gggaaacgcc ttttcggccg
      421 cggcagcgcc gccgctcgcg agctccgcgc agagcttttg gccgagctct ggcgcgaaat
      481 tttcgatgcg atcactcgcc gtgaagcgct gagggaggtc caacgccaga cgagacagcg
      541 gaacgccccg tcgtttcgcc tcggtcagca acgcgagttg cacgacgacc gggtcgcgtg
      601 tcggcagcgg cgagagcttg ccgcctccgg tcacggagag ctcgctcacg tgaaggaagc
      661 ccccgttcgc ctcgtagccg actacgcagc tttcaccgcc cgccttcgct tcgagcatcg
      721 cctcgatcac gaagggtgaa ccaatacggg tgcggcgggc cgagaaccag ctgctcttct
      781 cgagcacgct attgcagcta accggggccg cgacgaaggt cgcgccgaga tagcgcgccg
      841 cgagcacgca agcgacgtcg cctcgcaaga acgtgccgct ctcgtcggcg atgagcggcc
      901 tgtcgctgtc cccgtcggtc gaaaccaagg cgtcgagccg gtgctcgcga gcccactcgg
      961 cggcaagctt gacgtcctcc gcgcggattg cctccgtgtc gacgggaatg aagcgttcgg
     1021 accaaccgag acgcgtcacc tcggcgccga gctgctccag gatttccacc aagagctcgc
     1081 gcccgacggc ggagtgcccg taaacgccga cgcgtagccc ggcgagcgaa cgcggaaacg
     1141 ctcgcagcca gcgctgcacg taggcgcgag cgggctccgg atcggcggtg ggcagagagc
     1201 tcgagcccgc gcggagctga ccctcttcgt cgaataggtc tggcagctcc accgtctgcg
     1261 accgaatccc gatctcgtcg gctttcgaga tctcgccgag gccggtgttg aacttgattc
     1321 cgttgcgatc gtcggggatg tgacttcccg tgaccatgat cgacgggagc ctgtgcgcga
     1381 ggccgtgcag catcacggcg gggctcggta cgcgcccggc gtgcaccacc tcgagccccc
     1441 gcgtgtgaac ggcgcgcgcg accgccgaca tgatgcgctc ggtgctcggc cgcagatcgc
     1501 caccgacgac gacggcgtgc gcgccaccca gctgccccga ctgtgccaag tggttcaaaa
     1561 accccgcggt gtacgcgaac gcgatgcgat ccgtgaggtc gcgtaccagg ccgcgcgcac
     1621 cgctggttcc aaacttcacg cccgagcggg ccatcaagtc ggcgatgctg aacttgctca
     1681 tgggcgtgac ctcgactgga gttgctgtag cgcgagccgc ccggcacgca cgctgggggc
     1741 ggctcgcgtg tcggcaattc tcagatgctg gcgggctcga ccttgccggc gtcgacgccg
     1801 agctccgcga tggctcgggc aagctccgaa gcgctgatct tgccatcggc gcgcagccct
     1861 tcgagcgcgc cgatcgcgac gttctccgcg tccacttcga agtgacgacg cagcgcctcg
     1921 cgcgagtcgc tcatgccgaa accgtcggtt ccgagcggga tgacacgccc cgggatccag
     1981 cgtgagagca tgtccggcag cgccttcatg tagtcggagg cgaagacgaa cggcccttcg
     2041 accccggcga gggcttggct gacgtacggc acacgctgct cggcgtccgg atgcaggcgg
     2101 ttgtagcgct cgcaggcgag gccgtcggcg cggagctttt ggtagctcgt gacgccccac
     2161 acgtcagcgg agacgccgaa cttcttcagg agctcggccg cgcgcagcac ttcacgcatg
     2221 atcgagccgc tgccaaagag ctggacgtgg tgggccaggc gttgctctgc cttctggaag
     2281 cgatacatac ccgccaaaat cccgggcttc gcgccttcgg gcatgggcgg catcgcgtag
     2341 ttctcgttct ggagagtgag gtagtagtag acgttctctc cgtcgccgaa catgcgcttc
     2401 atgccgtctt cgatgatggc ggcgagctcg aacgcgaagg tgacgtcgta ggcgcggcaa
     2461 gccgggacgg tctgcgcaac gagctggctg tggccgtctt cgtgctgcaa accttcgccg
     2521 ttcaaggtcg tgcgcccggc ggtgccgccg aggacgaagc cgcgcgccat ttgatcgccg
     2581 gcggcccaga gctggtcgcc cgtgcgctgg aacccgaaca tcgaatagaa gatgtagaac
     2641 gggatcatcg gctcgccgtg ggtcgagtac gacgttgccg ccgagatgaa cgacgccatc
     2701 gagcccgcct cggtgatgcc ctcttcgagc acctgaccgt ccttgctctc gcggtagaag
     2761 atgagcttgc ccttgtcgat gggctcgtag agctgcccct gcgacgcgta gatgccgacg
     2821 gagctgaaca ggccctccat accgaaggtg cgcgcctcgt ccgggatgat cggcacgacg
     2881 cggcgaccga tgttcttgtc gcgaatcagc ttcgacagca ggcgaccgaa caccatcgtg
     2941 gtggacgcct cgcccttcga catccccttg tagaactctt cgtagagatc atccttcggg
     3001 agctcgagct ttacgtccac gctcacacgg cgctcgggca acatgccgcc gagcgcgcga
     3061 cggcgctccg cgagatacct cacctcgggg ctgttcggac ccgggtgata atacggcgga
     3121 tcctcgatga gatctttgtc gtcgacgggc acctcgagca cgtcgcggaa cgccttcagc
     3181 tcttcgttct tctcgaactt cttgcgcgag tgcgtgacgt tgctgccctc gaagccctgt
     3241 ccgaggagcc aacccttcac ggtgtgagcg aggatgacga cggggcggcc ctgacccgaa
     3301 agctcgaccg cgcgcttgaa cgccgcgtac accttgcgct ggctgtgccc accgcggcgt
     3361 agcttgccga gctcctcgtc ggacaagtgc ttgaccttct cgagcaagcg cggatccttc
     3421 gcgaagaact ccttgcgaac gacgtcgccg gtcgcggtcg tgaggcgttg ccactggccg
     3481 tcgacgacca tgttgagcgc ttcgcgcagc acaccgtcgt tgtcgcgcga gaagacgccg
     3541 tcccactcgg ggccccagat caccttcacg acgtgccagc cggcgccgcg gaacacgccc
     3601 tccagctctt gaatgatttt gccgttgccg cgcaccggtc cgtcgaggcg ctgcaagttg
     3661 cagttcacga cccaaatcag gttgtcgagg tgctcgcgcg tcgccgcggt caacgccccg
     3721 agcgtctccg gctcgtcgct ctcgccgtcg ccgacgaagc accacacgcg ctgctgcgag
     3781 gtgtctttga ggccgcgcgc ctgcaagtag cggttgaagc gcgcctgata gatcgagccg
     3841 atcggaccga ggcccatgct gaccgtgggg aactcccagt agttcggcat gagacgagga
     3901 tgcgggtacg acgacagccc cttgccgcgg tctgcttctc gtcggaagtg ttcgagctgg
     3961 tcgacgctga ccttccttcg aggaaggagc gcgcgtacat gcccggcgcc gcgtgaccct
     4021 ggtaataaac ctggtcgcca cggcccggcg cgtccttgcc gcggaagaaa tggttgaagc
     4081 ccgtttcgta gagcgttgcg ctcgacgcat aagtcgagat gtggccaccg atgccgtcgg
     4141 aagcgtggtt cgctcggtgc accatcacgg ccgcgttcca gcggatgatc gcactgatgc
     4201 gtcgctcgag atcgcgatcg cccgggaacg gcgcttcgtc ctcgacccga acggtgttca
     4261 cgtagtcggt cgtgagcggc agtagcggct cgacaccgct tcgtcgcgcg gactcgagca
     4321 ccctcgcaag cagatatcga gcgcggtcgc gcccttgttg ctcgatgacg gcgtcgagag
     4381 attcgaccca ctcgcgggtc tctagcgggt cacgatcgga gtcaggcctg tagctgtcca
     4441 cttcggagtc ctttggcggt gtgcgcatac ctatgccgga aagtgcctcc cttcaaggca
     4501 tcccgtactg tcggcccctc gaccgtcag
//
LOCUS       DASTKS010000154         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_693396, whole genome shotgun sequence.
ACCESSION   DASTKS010000154 DASTKS010000000
VERSION     DASTKS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693396"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..901
                     /locus_tag="VFQ35_03300"
     CDS             119..901
                     /locus_tag="VFQ35_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929681.1"
                     /translation="MKAPMPLSEDAIRLFASSLPESYRKNFEIGAIGQHARIALERES
                     APANAGLFLSARGQGDAICVCADDRPGLLATISAALVMCGLDVIEAEAYTRRLPDQHD
                     EAIDVFWVRHEALDRRNVRVSREEVDQLRQTLIGLLEGKIERRRLGDSARPGKPGPTP
                     GASETVVRFIENADGNFSTLEVETGDRSGLLLALSQALFNQRVQIIGSQVKTVKGRVY
                     DRFHIVEFDNSPISASRRLDIQVAVLSAVDPVIERQHAVSSV"
     gene            complement(915..>2522)
                     /locus_tag="VFQ35_03305"
     CDS             complement(915..>2522)
                     /locus_tag="VFQ35_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929682.1"
                     /translation="TGRKLELVERAAIRGALAPRALVASTRKLYIADQVTNALITVDK
                     GELSEQHQSPTCRRPSSLALAGAQLVVNCLFEHAISVRSLDALGLPGRESARIENAGP
                     FWAIAALEADGASWIAASGVEDRALERFDGAFGYVDSFASLFRVSGSRWERVWSANVS
                     ELSVLVPKALLLERTSPSHFAITVSSTGTDRLLRFDVDAHARTSSFESAPFAPGVTDL
                     LRVHERLYAASTLLDAWIAAASEPSGEPSLKQIASAPVAVNVDRRLGEALAFTSLIAP
                     HATSAGQNSRFTCETCHFEGGVDARVHHTGRGNVHVVTRPLFGLLRNAPHFSRALDPD
                     LTSVSHNEFRVAGLGNPDDPWFTLRTNEFPWLAALGVRTDVGPEALRRALLEFLAGYE
                     FPKNPRTRGRHRFSPDQARGAELFRDHCETCHAARLASDDSATHVGFEGWEPSLFELD
                     GPLVWARGDYERTGVLPYVNEKGTRVPSLRRVADKFPYFTNGSARSLEAVVARSARVG
                     GYFSHAAPDAEFEGDDARALVAFLELL"
BASE COUNT          461 a          817 c          824 g          420 t
ORIGIN      
        1 ccccctgcta cctcgggctc gctgcgttca aagtaactcc tagaagccaa gtaacgctcc
       61 tcatgaaaat tcacccggtc gaaaagaacc ggaaatcgca cgagctggag agtaagacgt
      121 gaaagcaccc atgccgttat ctgaagatgc cattaggctg tttgcgagct ctctccccga
      181 gagttaccgc aagaacttcg agatcggcgc gattggccag cacgcgcgca tcgcactcga
      241 gcgcgaatcg gctccggcca atgcggggct ctttctctcg gcgcggggtc aaggcgacgc
      301 gatttgcgtt tgtgccgacg acaggcctgg cttgctggcg accatcagcg cggcgctcgt
      361 catgtgtggg ctcgacgtca tcgaggccga agcgtacacg cggcgccttc ctgaccaaca
      421 cgacgaagcg atcgacgtgt tctgggtgcg tcacgaagcc ctcgatcgac gcaacgttcg
      481 ggtcagccgg gaagaggtcg atcagctgcg tcaaacgctg attggcttgc tcgaaggcaa
      541 gatcgagcgg cgccgactcg gtgactcggc gcggcccgga aagccggggc cgacgccagg
      601 cgcgagcgag acggtggttc gcttcatcga gaatgcggac ggcaacttca gcacgctcga
      661 ggtcgagacg ggcgatcgct cggggctgtt gctcgcgctt tcgcaggcgt tgttcaacca
      721 gcgggtgcag atcatcggct cgcaagtgaa gacggtgaaa ggtcgcgtct acgaccgctt
      781 tcacatcgtc gagttcgaca atagccccat cagcgcgtcg cgccggctag acattcaagt
      841 cgccgtgctc tccgcggtcg atccagtgat cgagcggcag cacgcggttt catcggtctg
      901 aagcgagtcg ggcgctacag gagctcgagg aaggcgacga gcgctctcgc gtcgtcacct
      961 tcgaattcgg cgtcgggcgc ggcgtgagag aagtagccgc ccacacgcgc cgaacgcgcc
     1021 accactgcct cgagcgagcg cgcgcttccg ttcgtgaagt acgggaactt gtccgcgact
     1081 cgtcgcagcg acggcacgcg cgtgcccttc tcgttcacgt aagggagcac gcccgtgcgc
     1141 tcgtaatcac cgcgagccca aaccagcggc ccatcgagct cgaacagaga tggctcccag
     1201 ccttcgaacc caacgtgagt cgccgagtca tcgctcgcga gccgcgctgc gtgacacgtc
     1261 tcgcagtggt cccggaagag ctccgcgccg cgcgcctggt cgggcgagaa gcggtgtctg
     1321 cctcgcgtcc tcgggttctt gggaaactcg tagcctgcca agaattcgag cagcgcgcgc
     1381 cgtagcgcct cggggccgac gtcggtccgc acaccgagcg cggcgagcca cgggaattca
     1441 ttggtgcgca gcgtgaacca agggtcgtcg gggttgccga gcccggcgac ccggaattcg
     1501 ttgtgactga cgctcgtgag atccgggtcg agcgcgcgag agaaatgcgg tgcgttgcgg
     1561 agcaagccga acaacggacg cgtgacgacg tggacgttac cgcggccagt gtggtgcact
     1621 cgcgcatcga cgccgccctc gaagtggcac gtctcgcacg tgaagcggct gttttgccca
     1681 gcgctggtcg cgtgcggcgc gatcaacgaa gtgaacgcga gggcctcccc caggcgtcgg
     1741 tcgacgttga ccgcaaccgg cgcggacgcg atttgcttca gcgacggctc accgcttggc
     1801 tcactcgcag ccgcgatcca agcgtcgagc aaagtgctcg cggcgtagag ccgctcgtgc
     1861 acgcgcagca agtcagtgac ccccggcgcg aacggcgcac tttcgaagct cgatgtccgc
     1921 gcgtgcgcgt cgacgtcgaa tcgaagcaag cgatccgtcc cagtgctgct caccgtgatc
     1981 gcgaagtggc tcggcgacgt gcgctcgagc aggagcgcct tcggaaccag aacggagagt
     2041 tcggagacgt tcgcggacca aacgcgctcc cagcgcgagc cgctcacgcg aaagagtgac
     2101 gcaaacgagt cgacgtagcc gaaagcgcca tcgaagcgct cgagcgctct gtcttcgact
     2161 ccgctcgccg cgatccacga cgcgccgtcc gcttcgaggg ccgcgatcgc ccaaaatggt
     2221 cctgcgtttt cgatgcgcgc tgattctcgc cccggcaagc ccaaagcgtc gagcgaccgc
     2281 acgctgatcg cgtgctcgaa gagacaattg acgaccagct gcgcgccggc gagcgcaagc
     2341 gagctcggtc gcctacacgt gggcgattga tgctgctcgc tgagctcacc tttatcgacc
     2401 gtgatgagcg cgttcgttac ttggtcggcg atgtagagct ttcgcgtcga ggccacgagc
     2461 gcgcgcggcg cgagcgctcc ccgtatcgcg gcgcgctcga cgagctccag tttcctcccc
     2521 gt
//
LOCUS       DASTKS010000155         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_471349, whole genome shotgun sequence.
ACCESSION   DASTKS010000155 DASTKS010000000
VERSION     DASTKS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471349"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFQ35_03310"
     CDS             complement(<1..1395)
                     /locus_tag="VFQ35_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929683.1"
                     /translation="MNRVGLASLTVLGCLAVTTLSAAQAGEPPPPPPPPPPPAANPAP
                     PAPAGAAPTAPAPAPTAPPTTPAPGATAPTESAAPVAPTMVAPTASASGNVTLDANAD
                     VEESDGKSKDTAAHSAQNWRKDSLQLQTGINASTGVLHVSQAGSGAPGTFRFSLLTGF
                     YSGTGFLCNSSSPCPAYGTDTATTADDVSAMSAHLGISATILPFLEGYIGFHNRATSN
                     SRGRPQLLEVLGDTNIGVKGFMPASPDQLFFFGGEAELWLLNGTGSVGLDGGGTSFTL
                     RGLATIDANNRINPEDRIPVRGHVNLGWRFDNSAALVDKLEHEPPPTGRGQRITRVER
                     FGLGINRVDFLQLGFGAEYVHSVIRPFLEWSMDIPANRQDYVCNIDVAKAHGESCLKL
                     ESGASTAPSRLTLGARLFPWEGRGLSVLAAFDVATSGSSVFIDETVPELPWNLWLGVA
                     YAVDTEPPKPIIKTV"
     gene            1732..2679
                     /locus_tag="VFQ35_03315"
     CDS             1732..2679
                     /locus_tag="VFQ35_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9929684.1"
                     /translation="MLIAVDQLRKEFRQGLFMRRVVAVKRVSFEVERGDIFGFLGPNG
                     AGKTTTIKMLTGLIRPSGGSARLFGELVPSAKARARLGFLPESPYIYPYLTPLEFVEL
                     SGRLAGMPGRLVRERAKKVLEQVGIAYAADRRVGRLSKGMLQRTGLAAALVSDPELLI
                     LDEPMSGLDPVGRKEVRDLILDERKKGRTIFFSTHILSDVEMLCDRVTILRRGEVVVS
                     GRLTELLKREVRRTDVAVAGAPPELVAELEAENHNVRRVGERIVIELEGEGTLPHVLQ
                     RTLSCGARVLEVVPRHESLEDLFVREAIEQGAPTRVTES"
BASE COUNT          438 a          799 c          955 g          502 t
ORIGIN      
        1 aaccgtcttg atgatgggct tcggcggctc ggtgtcgacg gcgtaggcga ccccgagcca
       61 caggttccac ggtagctccg gtacggtttc gtcgatgaag acgctgctgc cgctggtcgc
      121 cacgtcgaag gcggcgagca cgctgagccc tcggccctcc cacggaaaga ggcgcgcgcc
      181 gagcgtgagc cgcgatggcg cggtcgacgc gccgctctcg agcttcaagc acgactcgcc
      241 gtgggccttg gccacgtcga tgttgcagac gtagtcctgg cgattcgccg ggatgtccat
      301 gctccactcg aggaacgggc ggatgacgga gtgcacgtat tcggcgccga acccgagctg
      361 caggaagtcg acgcgattga tgccgagccc gaagcgctcg actcgcgtga tgcgctggcc
      421 gcggccagtg ggcggcggtt cgtgctcgag cttgtcgacc agcgcggcgg agttgtcgaa
      481 gcgccagcca aggttgacgt ggccgcgcac cggaatgcga tcttcggggt tgatgcggtt
      541 gttcgcgtcg atggtagcga ggccgcgcag cgtgaagctc gtcccgccgc catcgaggcc
      601 gaccgagccg gtgccgttca acagccagag ctcggcttct ccaccgaaga agaagagctg
      661 atccggcgag gccggcatga aacctttgac accgatgttc gtgtcgccga gcacctcgag
      721 cagctgcggg cggccgcgtg agttcgaggt cgcgcggttg tgaaagccaa tgtaaccctc
      781 gaggaagggt aagatcgttg ccgaaatgcc gaggtgggct gacatcgcgc tcacgtcgtc
      841 ggcggtcgtg gcggtgtcgg tgccgtaggc ggggcagggg ctcgacgagt tacacaggaa
      901 cccggtgccg ctgtagaacc ccgttagtag agagaaccgg aaggtgccag gcgctccgga
      961 acccgcttgg ctcacgtgga gcacgccggt cgaagcgttg atgccggttt gaagctgcag
     1021 cgagtctttg cgccaattct gtgcggagtg cgctgcggtg tctttcgact ttccgtcaga
     1081 ctcttcgacg tcggcgttcg catcgagcgt gacgttgccg cttgccgacg cggtgggcgc
     1141 gaccatcgtc ggcgcgactg gggccgcact ttcggtcgga gccgtagcgc ccggtgctgg
     1201 cgtcgttggg ggtgccgtcg gcgcgggtgc tggtgcggtg ggcgcagcgc cagcgggcgc
     1261 tggaggcgcc gggttcgctg cgggcggcgg aggcggtggt ggtgggggcg gcggctcgcc
     1321 ggcttgagct gcagagagcg tggtgacggc gaggcagccg agcaccgtaa gagaggcgag
     1381 accgacccta ttcatcgaga tcctcatagc cgtggtttgc ccgttagttc gggccccatg
     1441 acacccatgc tcttttagca tctttagcca gagtggtaac cttaaccgtt cggattcggg
     1501 ccgaagttgc gaacgcaaag cactcgcgct tcctcggacg aaaacacgag ggagtgctcg
     1561 gccggcgcgc cggctcggtt tgggctgatt tttgcgctgg tgcgagcgtt ttcgcgatga
     1621 aagccggctc tcggcgccaa tgcaagcgct ctcgcttttt tgcgaaatcc cgcgccttct
     1681 ataagctgtt cgcgaacggt agctcgatgc tcgagggttc tcttggctga agtgctcatc
     1741 gcggtcgatc agttgcggaa agagttccgc caaggcctct tcatgcgacg cgtcgtcgcg
     1801 gtgaagcgcg tctcgttcga ggtcgagcgc ggcgatattt tcgggttttt ggggccgaac
     1861 ggggcgggca agaccaccac catcaagatg ctcacggggc tcatccgtcc gagcggcggc
     1921 agcgcgcgct tgttcggtga gctcgtgccg agtgcgaagg ctcgggctcg cctcggcttc
     1981 ttaccggaga gcccgtatat ctacccgtac ctgacgccgc tcgagttcgt cgagctctcg
     2041 ggacgtctcg cgggcatgcc ggggcggctc gtccgcgagc gcgcgaaaaa agtgctggag
     2101 caggtcggca tcgcgtacgc agccgatcgc cgtgtcggtc gactctctaa aggaatgttg
     2161 cagcgtaccg gtttggcagc ggcgctcgtt tcggatccgg agctcctgat attggacgag
     2221 ccgatgagcg gcctcgaccc agtcggtcgc aaagaggtgc gcgacctcat cctcgacgag
     2281 agaaaaaagg gacgcaccat cttcttttcg acgcacatcc tcagcgacgt cgagatgctg
     2341 tgcgatcggg ttacgatttt gcgtcgcggt gaagtcgtcg tcagcggtcg cctgaccgag
     2401 ctcttgaagc gcgaggtgcg ccgcacggat gtcgccgtcg cgggcgctcc gccggagcta
     2461 gtggccgaac tcgaggcaga aaatcacaac gtgcgacgcg tcggcgaacg catcgtcatc
     2521 gagctcgaag gggagggtac gttgccccac gtgctgcagc gaacactcag ctgtggggca
     2581 cgagttctcg aagtcgttcc ccgtcacgag agcctcgagg acctcttcgt gcgcgaggcg
     2641 atcgagcagg gcgcgcccac ccgagtgacc gagagctagg ttcgcgctcg gctg
//
LOCUS       DASTKS010000156         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1808933, whole genome shotgun sequence.
ACCESSION   DASTKS010000156 DASTKS010000000
VERSION     DASTKS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808933"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFQ35_03320"
     CDS             complement(<1..287)
                     /locus_tag="VFQ35_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929685.1"
                     /translation="MLRPRYLFRLLTLCSSLVACGGSAAPEPKSTSVGSEDAPPRKVS
                     KAKAKELDASQVVLGLRGHEPELHECFSLAGEQSPVFVELEWDVDADGVVG"
     gene            538..879
                     /locus_tag="VFQ35_03325"
     CDS             538..879
                     /locus_tag="VFQ35_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9929686.1"
                     /translation="MSKFMYLFRTQSEFKPASPEEMERMMKAWTTWIDGLRAQGHLVQ
                     TGERLDRAGQVVRGKAKTVTDGPYAEAKDIVGGYMLVQANDLAQAVELSKGCPIFGID
                     GLVEVRPVIGM"
     gene            916..2184
                     /locus_tag="VFQ35_03330"
     CDS             916..2184
                     /locus_tag="VFQ35_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929687.1"
                     /translation="MTKVEPSLVDHLFRREAGKMVSYLTKLFGPSHLDLAEDIVQDAL
                     CRALQVWPVHGYPENPSAWLMRVAKHRALDLMRRARSLRAVTPELEVLLNEGAAHVDE
                     LALDREIQDDQLRMMFSCCHPALSSDAQVTLILKTLCGFSVAEIANALLVSEDSVEKR
                     LGRARKVLRDSKSLMTIGTPGEIAGRLEAVYQAIYLLFNEGYHGSQSEDTVREELCYE
                     ALRLGMLLTEHPSSARPKTFALLALFCFHAARLPGRVDQDGCLLQLAMQDRAKWDREL
                     IGEGLRYLARAAVESELSDFHVEAAIASLHSRARTYEETDWLRIKELYDLLYGMRRSP
                     VVALNRAIALGHLEGPERGLEALGVLESRERLASYPFFFAALGEFQRALGRWDEAERS
                     FQRAAALARNPAEQRFLQARADAAQNRARG"
BASE COUNT          405 a          722 c          749 g          397 t
ORIGIN      
        1 tcgccgacga ctccgtccgc gtccacgtcc cattcgagct ccacgaagac ggggctttgc
       61 tcgccggcca gggagaagca ctcgtgcagc tccggttcgt ggcctcgtag tccgagcacg
      121 acctggctcg cgtcgagctc cttggctttc gccttcgaaa ccttccgcgg cggagcatcc
      181 tcggatccga ccgaggtgct cttaggttcc ggagccgcgc tcccgccgca ggcgactaga
      241 ctcgagcaga gcgtcagcag ccggaagaga tacctcgggc gcaacatcgg aacgctgaga
      301 tagcctcgac ggttcacgcc agcaaacgca ggaacgcgca aaatctcacc gaccagtgcc
      361 taagtttcag tgcgagcgaa cgcttccgcg aaggacggta gcggcgtcgt tcgcaaaaag
      421 atgtctgggt tcgcctggcc cacacgtcgt gggggtggag atggcaaacc gagccgctct
      481 gccagcccgg tccggcgccg gccctggggc attggttcga gccggaggag agttcgtatg
      541 tcgaaattca tgtacctttt tcgaacccag agtgagttca agccagcctc gcccgaggaa
      601 atggagcgga tgatgaaggc gtggaccacg tggatcgacg gcctgcgagc ccaaggacac
      661 ctggtccaaa cgggagagcg cctggatcgc gcgggccagg tcgtccgggg taaagccaag
      721 acagttaccg atggtcctta cgccgaagcc aaagacatcg tgggcggcta catgctcgtg
      781 caagccaacg acctggcgca ggcggtcgag ctctccaagg gctgccccat cttcggcatc
      841 gacggcctcg tcgaggtgcg acccgtcatc ggcatgtgag cgcgtgtcga ccagacgatt
      901 ttgccaactt ccgacgtgac gaaggttgag ccatcgctcg tggaccatct tttccgtcgc
      961 gaggcgggga agatggtctc ctatttgacg aagctcttcg ggccgtctca cctcgacctg
     1021 gcggaagaca tcgtgcagga cgcgctctgt cgagcgctcc aggtttggcc ggttcacgga
     1081 tatccggaga acccgtcggc ctggttgatg cgcgtcgcga aacaccgcgc cctcgacctg
     1141 atgcgccgcg cgcggagctt gcgtgccgtg acgcccgagc tcgaggtgct gctgaacgaa
     1201 ggcgccgcgc acgtcgacga gctcgcgctc gatcgcgaga ttcaagacga ccagctccga
     1261 atgatgttct cgtgctgcca ccctgcgctg tcgagcgacg cgcaagtcac cttgatcctg
     1321 aagacgctct gcggcttcag tgtggcggag atcgcgaacg ccctcctggt cagcgaagat
     1381 tcggtggaga agcgcctggg tagggctcgc aaagtgctgc gcgactcgaa gagcctgatg
     1441 acgattggga cgccggggga aatcgccggg cgcctcgagg cggtctatca agccatctac
     1501 cttttgttca acgagggcta tcacgggagc caatccgagg acacggtgcg tgaggagctc
     1561 tgttacgagg cgcttcgcct cgggatgttg ctcaccgagc acccgagcag cgcgcgcccc
     1621 aaaaccttcg ccttactcgc gctgttctgc tttcacgcgg cgcgtctgcc cgggcgcgtc
     1681 gaccaagacg gttgcttgct ccaactcgcg atgcaggatc gagccaagtg ggaccgagag
     1741 ctcatcggcg agggattgcg ttacctcgcg cgcgcggcgg tcgagagtga gctgagcgat
     1801 tttcacgtcg aggccgcgat cgcttctctg catagccgcg ctcgaacgta cgaagagacg
     1861 gactggctcc gcatcaagga gctctacgat ctgctgtacg gcatgcgccg ttcaccggtg
     1921 gtcgcgctga accgagccat cgcgctcggg cacctcgaag ggcctgagcg cggactcgag
     1981 gcgctcggtg tgctcgagag ccgcgagcgc ctcgcgagct acccattctt tttcgctgca
     2041 ctcggcgagt ttcagcgcgc gctcgggcgt tgggacgagg cggaacgtag ctttcagcgc
     2101 gcggccgcgc tcgctcgaaa tcccgctgag cagcgcttct tgcaggcgcg cgccgacgcg
     2161 gcacagaacc gcgcccgggg ctgagtcgac cgacgctctc gagtcgtcac gctgcgcgct
     2221 cgggagcgcg gggcgcgagc gataggctcc gacgcgcgcc gtctcgcaag agg
//
LOCUS       DASTKS010000157         4680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1808963, whole genome shotgun sequence.
ACCESSION   DASTKS010000157 DASTKS010000000
VERSION     DASTKS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808963"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..551
                     /locus_tag="VFQ35_03335"
     CDS             99..551
                     /locus_tag="VFQ35_03335"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET9929688.1"
                     /translation="MSPDRIDWLEAKTMVWRILMASGVGLALSWVAAVLFLAFMRPRG
                     SLLQEALRLLPDTLRLLRRIATDSSLPVGIRVRLWLLFGYLAFPFDLVPDFIPVIGYA
                     DDAIIVVLVLRSVVRRAGAEAVRRHWPGSPQGLAALWRAAQLPGAPEA"
     gene            complement(773..1075)
                     /locus_tag="VFQ35_03340"
     CDS             complement(773..1075)
                     /locus_tag="VFQ35_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929689.1"
                     /translation="MLKNALFFALTCSCAGVLACSRDRTNDSMVPAGSSAEPRPVGID
                     ETTTPRGAARAQNTEPLPGNTGTPTPPAGITSGTTTGGSGGATGLTSGGHGGQPVR"
     gene            complement(1087..1635)
                     /locus_tag="VFQ35_03345"
     CDS             complement(1087..1635)
                     /locus_tag="VFQ35_03345"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HET9929690.1"
                     /translation="MASESREPGFIERATGTLVLGASAGIVATLAMSALMYGEQRLGL
                     LGRAPPRHIVEHALARFGLRRSQIPKSVRAGLTALAHLGFGASQGALYALLQDVASRR
                     ISALKTPSAITGVPFALAVWAASYAGWIPALDILPKPRNDRPMRPTSMVLAHIAYGVT
                     LARVLRLSRERRTRSRVNLRTT"
     gene            1942..3849
                     /locus_tag="VFQ35_03350"
     CDS             1942..3849
                     /locus_tag="VFQ35_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9929691.1"
                     /translation="MRNTRAFKGVPNGPAAIRGALLGSLLFGTLVFTVTGCSDENGGT
                     SGGTGGKGGAGAAGGNGGASPLPSANGELEVVGHILKPEQLSPPDVSKLRVPPGFVIT
                     KAAENLGNARMLAVGSDGGIYVTRREQGDVLLLKDGGNGELLPPVRAASRSGVHGLAL
                     YQGKAYLATPNEIFQGDILPDGTFGPLEMLIHDLPDAGQHNTRTVQIGPDEMMYISVG
                     STCNECNEPNPENATILRASLDGKQRAIWASGLRDTIGWGWHPQTGELWGMDQGIDWL
                     GDGIPPEELNRIVRGNRYGWPYIWGDNQINPRLDPPEGLTKEEWRKTSTPLVLGYTSH
                     AAPMQMSFYDGAQFPLEYRGDAFVSMHGSWNRKPPAGYEVVRVHFNQGEAQSIEPFVT
                     GFATAEGEHGRPCGNAVAPDGSLLFTDDRNGVLYRVSYTGAPNGLSPSAIPPDAMLEQ
                     AARGHDVPLAMERPETSADAAIMVASAAFTQGGPIPATYSEYEQGASFPVAWSAGPDT
                     TVSYAVIMEDPDAKMPKPFVHWVVWNIPAALLELREGLQEQDILTDPRGLRQGVTSRG
                     NVGYLGPRPPAGDPPHTYHVQVFALDTILEVPISGASRDTLLGAMAGHVVARGELVGT
                     FARPATQVSRP"
     gene            complement(3893..>4680)
                     /locus_tag="VFQ35_03355"
     CDS             complement(3893..>4680)
                     /locus_tag="VFQ35_03355"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929692.1"
                     /translation="EVAVGLALLRLSSTQSVDGVELALAGQAAEHEYVGTLCGIMDQF
                     AASLSRADSALLIDCRSLAARPVPMELGTACILICDTRVKHRLADSAYNQRRAECNRG
                     VELLQQRFPHVRSLRDASLGDLAAVPLPDVIARRCRHVVSENERTLQAADALVEGRLA
                     DMGALMSKSHDSLRDDYEVSCDELDEAVRAAQGEARVYGSRMTGGGFGGCTITLLERD
                     AVGRVREAIERRFAERFSTKPAFFTSRAAEGAREHAATESTLA"
BASE COUNT          789 a         1494 c         1572 g          825 t
ORIGIN      
        1 cgtgcttcgc agccgcagga tcttggctcg cagcagggcg gggaactcgg gacaccccct
       61 aggcgcggtt tcttgattcg ctccagctgg tggagcagat gagtcccgat agaatcgact
      121 ggctcgaagc caaaaccatg gtctggcgaa tcttgatggc gagtggggtc ggcctagcgt
      181 tgagctgggt ggccgcagtg ctttttctcg cgttcatgcg accgcgcggt tcgctgttgc
      241 aggaagcgtt gcgtctgctg ccggatacgt tgcgtctact gcgacgcatc gcgacggact
      301 cgagcctccc ggtgggtatt cgtgttcggc tctggctctt gtttggctac ctcgcgtttc
      361 cgttcgatct cgttccggac ttcatccctg tcatcggtta cgcggacgat gccatcatcg
      421 tcgtcctcgt gctgcgctcg gtcgttcgcc gcgccggcgc agaagcagtg agaaggcact
      481 ggcctggctc accccagggg cttgcagcgc tttggcgcgc agcgcaattg ccgggtgcac
      541 cggaagctta gcagggcgtt gaaaaacgcg ccctagcgag gcgggccgcg ttaggagaga
      601 gcaccgcagg cctcctgaag caggtcgagg agcgcaacga cgagcccgcc cgccgcagca
      661 agaggatcgg cgcgcgcagt atgactgccc attcgcgggc tcttaggcgc ccgaaggtgt
      721 gcgagccgct ccacttgcta cggcggacgc ttgctcgcga gctcactggc gctcagcgca
      781 ccggttgccc gccgtgaccg ccgctcgtga gaccggttgc gccccccgac ccgccggtcg
      841 tcgtgccgct cgtaattccc gcaggcggcg tgggtgtgcc ggtgttgcct ggcaacggct
      901 ccgtgttttg cgctcgggcc gctccgcgcg gcgtcgtggt ctcgtcgatt ccaaccggtc
      961 gcggttcggc gctggagcct gcgggaacca tgctgtcgtt cgtgcggtcg cggctacacg
     1021 cgagcacacc agcgcatgag caggtgaggg cgaagaagag agcgttcttg agcatgtcgt
     1081 gttctttcaa gtcgttcgaa ggttgactcg agagcgcgtg cgccgttcac ggctcagcct
     1141 gagcactcgc gcgagcgtca cgccgtacgc gatgtgcgcg aggaccatcg acgtgggtcg
     1201 catcggtcga tcgttgcgcg gcttcggtag gatatcgagc gccggtatcc agcccgcata
     1261 gctcgctgcc cagaccgcca gcgcgaacgg gacgccggtg atcgccgacg gcgttttgag
     1321 cgctgagatt cggcggctcg cgacgtcttg gagcaatgcg tagagcgcgc cttgggaggc
     1381 gccgaacccg aggtgtgcga gcgccgtgag cccggctcgt accgacttcg gaatctggct
     1441 cctgcgaagt ccgaagcgcg cgagggcatg ctcgacgatg tgccgcggcg gcgcgcgacc
     1501 gagcagtccg agccgttgct cgccgtacat gagggccgac atggcgagcg tcgcgacgat
     1561 gccggcgctc gcgccgagga ccagagtccc agtggctcgt tcaatgaacc ccggctctct
     1621 cgattcagat gccatggctg cagcggagca cgcaccgtgc cactgcagcg ttcgtaagcg
     1681 tccgtcgacc gactggttcg aggcggcaaa tcagcgtgcc cggcgctccc caccgcgcgt
     1741 gtggactaaa gggcgttgcg cgcactgcgg ctgcatctac atctttggcg cgctgccaca
     1801 tgcaaagcga tcacacgctc gacgacactt cagtttgtcc aaccgtggct ctcgaacggc
     1861 atcgaagttg cagcccacgc cagccgctcc cagcgcgatg cactgccgcg ccgtcgacgg
     1921 agcgttggag caggtggagc gatgcggaat acacgagcgt tcaagggagt tccgaatggt
     1981 ccggccgcga ttcgcggcgc tctgttggga agcttgttat tcggcacgct cgtcttcacc
     2041 gtcacgggat gcagcgatga gaacggtggg accagcggcg gaaccggtgg gaagggcggt
     2101 gccggagcag cgggcggcaa cggtggtgcg agccctctgc cgagcgcgaa cggagaactc
     2161 gaagtcgtcg ggcacatttt gaagccggaa cagctgtcgc cgccggatgt gtcgaagctc
     2221 cgcgtgcctc ccggtttcgt gatcaccaaa gccgcagaga acctgggaaa tgctcggatg
     2281 ctcgcggtgg gcagcgatgg cggcatctac gtcacgcgcc gtgagcaagg ggatgtcctg
     2341 ttactcaagg acggaggaaa cggcgagctc ctgccgccgg tgcgcgctgc ttcgcgctcg
     2401 ggcgtccacg ggttagcgct ctatcagggg aaggcatacc tcgccacgcc gaacgaaatc
     2461 ttccagggcg acatccttcc cgacgggacg ttcggaccgc tcgaaatgct cattcacgac
     2521 cttccggatg ccggtcagca caacacgcgc acggtgcaga ttggtcccga cgagatgatg
     2581 tacatcagcg tcggctcgac gtgtaacgag tgcaacgaac ccaacccgga gaacgcgacc
     2641 attctgcgtg cgtcgctcga cggcaagcag cgcgcgatct gggcttctgg gttgcgcgac
     2701 acgatcggct ggggctggca cccacagacg ggggagctgt ggggcatgga tcaaggtatc
     2761 gattggctcg gggatggcat tcccccggaa gagctgaacc ggatcgtgcg cgggaaccgt
     2821 tacggctggc cctacatctg gggcgacaac caaatcaatc cacgcctcga cccgcccgaa
     2881 gggctcacga aggaagagtg gcggaagacg agcactcccc tcgtcctcgg ctacacctcg
     2941 cacgccgcgc cgatgcagat gtcgttctac gacggcgcgc aatttccgct cgagtatcgc
     3001 ggggacgcgt tcgtctcgat gcacggttct tggaatcgaa agccgcccgc cggctacgag
     3061 gtcgtgcgcg tccacttcaa tcagggcgag gcgcaatcga tcgaaccgtt cgttacgggc
     3121 ttcgcgacgg cagagggcga acacgggcga ccgtgcggta acgcagttgc tccggatggc
     3181 tcgctcctct tcacggacga ccgcaacgga gtgctctacc gagtttcgta cacgggtgcg
     3241 ccgaacgggc tgtcccccag cgcgatccca cctgacgcca tgctcgagca agccgcgcgg
     3301 ggacacgacg taccgctcgc gatggagcgc cccgaaacca gcgcagacgc tgcgatcatg
     3361 gtggcttccg cagcgttcac ccagggcggc ccgatcccgg cgacctattc cgaatacgag
     3421 cagggcgcga gttttccggt tgcgtggagc gcggggccgg atacgaccgt ctcctatgca
     3481 gtcatcatgg aagacccaga cgccaagatg cccaagcctt tcgttcattg ggtggtttgg
     3541 aacatccccg cggccttgct cgagttgcga gaaggtttgc aagagcagga cattttgact
     3601 gacccgcgtg ggctgcggca gggggtcacg tcgcgaggca atgtcggtta ccttgggccg
     3661 cgtccaccgg ctggcgatcc gccgcacact tatcacgtgc aggtgtttgc tctggacacc
     3721 atcttggagg tgcccatcag cggcgcgagc cgcgacacgt tgcttggtgc catggccggg
     3781 catgtcgttg cgcgcggtga attggtcggg acttttgcgc gaccagcgac gcaggtgtcg
     3841 cgaccttgag cacgcgagac gcgcgctcgg cggctccctg cacggagaac tcctaggcca
     3901 gcgttgactc ggtcgccgcg tgctctctgg cgccttcggc agcgcgcgaa gtgaagaacg
     3961 ccggtttggt cgagaagcgc tccgcgaagc gccgttcgat ggcttcgcgg acccttccaa
     4021 ccgcatcgcg ttccaagagc gtgatggtgc agccgccaaa gccgccgccc gtcatccgcg
     4081 aaccgtacac ccgagcttcg ccctgggccg cgcgaacggc ttcgtcgagc tcgtcgcagc
     4141 tcacttcgta gtcgtcccgc agcgagtcgt gcgacttcga catgagcgcg cccatgtcgg
     4201 cgaggcgtcc ttcgacgagc gcgtccgcgg cttgtagcgt gcgctcgttt tcactgacga
     4261 cgtggcgaca gcgccgcgca atcacgtccg gcaacgggac cgccgccaag tcgcccaggc
     4321 tcgcgtctcg cagacttcgg acgtggggaa accgctgctg caagagctcg acgcctcgat
     4381 tgcactcggc gcggcgctgg ttgtaagccg agtccgctag ccgatgcttg acgcgcgtgt
     4441 cgcagatcaa gatgcacgcg gtgccgagct ccattggaac cggccgagct gcgagtgaac
     4501 ggcagtcgat gaggagcgcg ctatcggcgc gagacagcga agcggcgaat tggtccatga
     4561 tgccgcacag ggtccccacg tactcgtgtt ctgccgcctg gcctgcgagc gcgagctcga
     4621 ccccatcgac cgactgcgta ctcgacaaac gcaggagcgc gaggcccacc gcgacctcca
//
LOCUS       DASTKS010000158         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_471614, whole genome shotgun sequence.
ACCESSION   DASTKS010000158 DASTKS010000000
VERSION     DASTKS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471614"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFQ35_03360"
     CDS             <1..735
                     /locus_tag="VFQ35_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929693.1"
                     /translation="VCVVYVAELELLGTRSSSAGYECYTPDGRRRVLRASGAVAVALE
                     PRHITLSFDTQAGPFSYRLERTNDAFAQHRPSPRLGWQILTAMSPARATGVLDEGELL
                     GFGYSDRVELGAPPRALGLSELAWGRGRTGSENFVFTEARFRDRSVFRRAVLNGASLD
                     DFALARGERSLELALGASRFELEEERCLHAGNAVDEQRFPSALERSLSRVLSGNVHEM
                     RALACVRGKNHESGLALHERVYFGAH"
     gene            736..1407
                     /locus_tag="VFQ35_03365"
     CDS             736..1407
                     /locus_tag="VFQ35_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9929694.1"
                     /translation="MSRWARWRYFAWTLLAYALWAGCFELVGNVAARLPTHDLRTSWD
                     SRIPLVPAAIWPYEACYLLPFVIPLVARDAQRVRVGLVAAVVANLAAFFVYLAYPVAF
                     PRPSLGSSLSERVLALEYALDFQPGANNLPSLHVAMSWLILITCHRQGLSRAAFCALT
                     LLTLAITASTLLVKQHLVYDAIVGVVWAFGSFALAQSLHRAWTSKRAALIRPDLEAGA
                     HFHKQ"
     gene            complement(1389..1892)
                     /locus_tag="VFQ35_03370"
     CDS             complement(1389..1892)
                     /locus_tag="VFQ35_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929695.1"
                     /translation="MTRGGTAAGGTSSSRGGAASGGNRAEGGAGVVAGRSGFGGASGG
                     IPGLPSMAGFPAMLPPPAQSNGNSPYLRPCHGDTSSCVDVANLRCLGIRDTEVRGYSC
                     SNPCSDSSDCSTAPTLVAARAECVDFTTQKHCLLVCLENGEQRECPAGMTCYNYPGNP
                     VGYCLWK"
     gene            2278..>3359
                     /locus_tag="VFQ35_03375"
     CDS             2278..>3359
                     /locus_tag="VFQ35_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9929696.1"
                     /translation="MSAFCIIGCSSGSDQDGSSGAGGSSTSPIALDGAGRPNGGGTQV
                     GGGNGASCGTGDPLYDCTGVSFESESIPLDIYILFDQSGSMLNDVGGITRLAAVQAAV
                     ASFLRDPKSQGIGVGIGYFGYQPIGQVECDAARYVPDVAVSNDHDLVIDSLDSRMPTG
                     ETPTPAALEGACRYASSYKSKNPGRNVVILLVTDGKPEAPVSCASGGCCPTLDGAITA
                     ATRCRQGTPQISTYVLGVGPNLDNLHAIAQAGGTKTAYLVGDQDVSSNVLGALNDIRR
                     DAQIPCNIEIPPAAPNRVVDYDQVNVSSSKGGCSSTIYRVASAAECTPQGGWHYDDPT
                     RPSHIELCPASCALVNSASVGLKVLVG"
BASE COUNT          551 a         1135 c         1051 g          622 t
ORIGIN      
        1 gtgtgcgtcg tgtatgtcgc ggagctcgaa ctcttgggga cgcgctcgag ctccgcgggc
       61 tacgaatgct acacgccgga tgggcgccgt cgcgttctgc gagcaagcgg cgccgttgcg
      121 gtcgcgctcg agccgcgtca catcaccctc tccttcgaca cgcaggcggg tcccttcagc
      181 tatcgcctcg agcgaacgaa cgacgctttc gcgcagcatc gcccctcgcc tcgtcttggc
      241 tggcagattt tgaccgcgat gtctcccgct cgcgcaaccg gcgtcttgga cgaaggcgag
      301 ctgctcgggt ttggctactc ggatcgggtc gagctcggcg cacccccgcg ggcactcggc
      361 ctctccgagc tcgcctgggg gcgtggacgc accggctccg agaacttcgt cttcaccgag
      421 gcgcgtttcc gcgaccggtc cgtgtttcgg cgcgcggtcc tgaacggcgc ttcgctcgac
      481 gatttcgcgc tggctcgcgg cgaacgcagc ctcgaactcg ccttgggcgc atcgcgtttc
      541 gagctcgaag aagagcgctg ccttcacgcg ggcaacgctg tcgacgaaca gcgctttccg
      601 agcgcactcg agcgcagcct gtcgcgagtg ttgagcggga acgtccacga gatgcgcgcg
      661 ctcgcctgtg tgcgcggcaa gaaccacgaa agcgggctcg cgctgcacga gcgcgtatac
      721 ttcggagccc actaggtgtc gcgctgggcg cggtggcgct acttcgcgtg gacgttgctc
      781 gcgtatgccc tttgggccgg ctgcttcgag ctcgtcggca acgtcgcagc gcgcctgcca
      841 acgcacgacc ttcgcacctc gtgggatagt cgaatcccgc tggtccccgc cgctatttgg
      901 ccctacgagg cgtgctacct gcttccgttc gtcattcctc tcgtcgcacg cgacgctcag
      961 cgggtgcggg ttggcctcgt cgcagccgtg gtcgcaaacc tcgcggcatt tttcgtctac
     1021 ctcgcgtacc cggtcgcttt tccgcgaccg agcctcggga gctcactgtc ggagcgcgtg
     1081 ctcgcccttg agtacgctct ggattttcaa ccgggcgcca acaacctgcc gagcctacac
     1141 gtcgcgatga gttggctcat tctcatcact tgtcaccggc aaggtctctc gcgcgcggcg
     1201 ttttgcgctc tcaccctgct cacactcgca atcaccgcct cgacgctgct cgtgaaacag
     1261 cacctcgtct acgacgccat cgtcggcgta gtctgggcat tcggctcttt cgcgctggcg
     1321 caaagcctgc atcgcgcttg gacctcgaag cgagccgcgc tgattcgccc cgacctcgag
     1381 gcgggcgctc acttccacaa gcaatagccg acggggtttc ccgggtagtt gtagcaagtc
     1441 atgcccgccg ggcattcgcg ctgctcaccg ttctcgagac agaccagcaa gcagtgcttt
     1501 tgcgtggtga agtcgacgca ttcggctcga gccgcgacga gcgtgggcgc cgtggagcag
     1561 tcgctcgaat cgctgcacgg attcgagcag ctatagccgc gaacctcggt gtcgcggata
     1621 ccgaggcaac gcaagttcgc gacgtcgacg cacgagctcg tgtcgccgtg acacggccga
     1681 aggtagggtg agttcccgtt cgactgcgcc ggcggcggga gcatcgcagg gaacccagcc
     1741 atggatggca atccgggaat gcctccgctc gcgccgccga aacccgatct gccggcgacc
     1801 actcccgcgc cgccttcggc tcggttgcct ccgcttgcag ccccgccacg actcgatgaa
     1861 gtgccgccag cggccgtgcc gccgcgtgtc atcgtcgtgg agctcgtgac ccctccggag
     1921 gcggtccccc cgtcgctcgg atttgcgtcg ctgtctcgcg gcccactact cgagcagccc
     1981 agggcgagca cgagcgttga aagagcagtt cgtcgacgcg aaaggggtag gggcatcgtt
     2041 cctcacactc gtcgcgagtc aaaagtccgc gccgcgctcg cgcaaaaagc gagcggatat
     2101 cggcacgatt tccactatgg gggcgcccgg accgatttct gatcgattcg gtctccgacc
     2161 gacaaaaaaa taaaatcgtc aggttggtca gaaatgggtc gggccatggc cattcagcaa
     2221 gcgaaggagc gccacccctt gctgagccat gtcctaagtc gatcgctcgt tgtcctcgtg
     2281 agcgcttttt gcatcatcgg ctgctctagc ggatccgatc aggacgggag ctcgggagcg
     2341 ggtggctcct cgaccagtcc aattgcgctc gacggtgctg gaagaccgaa cggaggagga
     2401 acccaggttg gcggcggcaa cggtgcgagc tgcggcacgg gcgacccact ttacgactgc
     2461 accggtgtca gcttcgagag tgagagcatt cctctcgaca tctatatctt gttcgaccag
     2521 tcgggctcga tgctcaacga tgtcgggggc atcacgcgcc tcgccgccgt tcaggcagcg
     2581 gtcgcgtcgt tcctgcgcga tccgaagagc cagggaatcg gtgtcggcat cggctacttc
     2641 ggctatcaac cgatcggcca agtcgagtgc gacgctgctc gatacgtccc cgacgtggcg
     2701 gtatcgaacg accacgatct cgtcatcgac tcgctcgatt cgcgaatgcc gaccggagaa
     2761 acgccgactc ccgccgcgct cgaaggcgcc tgccgctacg cttcgagcta caagtcgaag
     2821 aaccctggcc gcaacgtggt gatcctgttg gtcaccgacg gcaagcccga ggcgcccgtt
     2881 tcttgcgcgt cgggcggttg ttgcccgacc ctggacggcg cgatcacggc tgccacgcgc
     2941 tgcaggcaag gcaccccgca gatctcgact tacgtgctcg gcgtcgggcc caacctcgac
     3001 aatctgcacg ccatcgccca ggccggcggc acgaagaccg cctatctcgt cggtgaccag
     3061 gacgtgagca gcaacgtgct cggtgcgctc aacgacattc gtcgcgacgc gcagatcccc
     3121 tgcaacatcg agatcccacc cgccgccccg aaccgcgtag tcgactacga ccaagtgaac
     3181 gtctcttcct cgaaaggggg atgttccagc acgatctacc gcgtggcatc ggcggcggag
     3241 tgcactcccc agggtggctg gcactacgac gacccaacga ggccgtcgca catcgagctc
     3301 tgtccggcaa gctgcgcact cgtgaacagc gcgagtgtcg gtttgaaagt tttggtcgg
//
LOCUS       DASTKS010000159         7675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_246436, whole genome shotgun sequence.
ACCESSION   DASTKS010000159 DASTKS010000000
VERSION     DASTKS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246436"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFQ35_03380"
     CDS             <1..244
                     /locus_tag="VFQ35_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929697.1"
                     /translation="VSSGTVLHEDTLDPWRVASWTRVRSGLDTARVRPTPQRAARSSP
                     AVSSGLDVPATSATDEAHSWIHSDIIDPWAGRALRQ"
     gene            complement(437..1180)
                     /locus_tag="VFQ35_03385"
     CDS             complement(437..1180)
                     /locus_tag="VFQ35_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929698.1"
                     /translation="MSRRFFFIVAGLALLACEPRRRDALRVVGPKAEVAAAPAPDGLF
                     SRAEGRLHRQLPERPRQEHQRCPDETLAHGQSEQSSVLVRLRDDRLDRKNIMPLELLQ
                     TLGADPLARFRDSPRRTKAEDEALESLSERRYLAELVVRVFSPPTHFRRLNALHSEWS
                     GGRFDAELVVYDLENDSVLCSAKVQARGDATGAPLSRRLRESTRADLERKLYARALRE
                     VNGALSSITRVLRWPEEALALSRNEEPSN"
     gene            2014..4245
                     /locus_tag="VFQ35_03390"
     CDS             2014..4245
                     /locus_tag="VFQ35_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9929699.1"
                     /translation="MNTPSFPPGPDAARWIRGWRLDRLIHVGKTACVYRATSEAGVVG
                     AVKALRPAAIVHRTAAERFAREIAALSGLTHPAIPRLLDQGIDDEGLPWFVTEWIVGW
                     DLETHRVRLGGRLLVEEVFDVGVRLLEALAHAHAVGFIHRDVKPANLFLTETGELKVL
                     DFGLCRGLVAASDFGITGNVVIGTPAFMAPEQAGRRWAQVDARTDIWSVGATLFLLAS
                     GQPVHLGDPEQQRKASYRADARSLASVDASLPFGLVTVIDRALRRDPLERFQNATEMH
                     EALLEARTPGKPKLRTAPVMASTANDPHRHTTEQDRELYAPSQRFLSSALRLVSSPDQ
                     TTLRERTLSQQSELLIGRDAGNSGWSIGDTRVSRLHARIAWDDRQGCYRAADLQSTNG
                     MRVNGLRRTTTTLSHGDVVRVGDSVLLLTERTTMQDLERAAEHAARSTATLLLHGETG
                     VGKEVLARQIHAWSERRGAFVAVNCGALPRDLAASELFGHAKGAFSGAANARRGVFPA
                     AQGGTLLLDEVGELPLELQPLLLRALQERAIRPVGADMEMPIDVRVLAATNVQLEQAV
                     NEGKFRADLYARLAQFPINIPPLRERREQILDLARVFARGAGHELTLAADAAEALLLW
                     HWPFNVRELENLMRRWCMLARPGAVLGLDFLHSVNPAMTSTLQDRASTPPAASSSSGT
                     QRNPLSDRTQLESLLHACDGNVSEVARRLNTTRAQVYRWLERFGIEPSRARGDGGRRR
                     GND"
     gene            4294..4905
                     /locus_tag="VFQ35_03395"
     CDS             4294..4905
                     /locus_tag="VFQ35_03395"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929700.1"
                     /translation="MKQLHRADLYGWSRFDESRNIDFHSLLWRRPEGNVVIDPLPLSE
                     HERGRLQEFGGVKHVVITNSDHVRATAELVALTGARTYGPRGERGSFPLQCDVWLGDE
                     DTVVEGLVALALEGSKTDGELALLLERTTLITGDLVRAHAGGRLDSLPEAKLRDRAAA
                     RRSLERLAGIASVVAVLPGDGWPMFRDGHGALTDLVRRFGLEA"
     gene            5212..6843
                     /locus_tag="VFQ35_03400"
     CDS             5212..6843
                     /locus_tag="VFQ35_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929701.1"
                     /translation="MQALSLPEPLPDDPEVVATALETAEIFGVQGDVREAVRWVRRAA
                     ELAGDAGNDDRALVLARAAADLTATMNDRESAAAPPPAAPPPPPQAEASPADAAPAEP
                     ARAAPPPPPPPPPPPPPPPPPPPITAGSTRPSTRPASDSRAPQAVSVSPIVSLNSESD
                     TSSSEVRAPALDANGSNGASASPEPPREVVAAQASGSPSSSSAKPAVSHPHDGLDDLF
                     DEPPTGEHRAAAAAAPVAPAPVAASVALGGLPSLSATTGGIGPAGALGTLQSHTVSRE
                     ALANAATVEAPVLRPLLKPPSKPPRPVTSTVLGGAAASVSAPNPAPVAVPPASAPPIV
                     GSPVIAPPLAAPVVAPPVAAPAPIVASAPAPEPSAERDAPPVTVAAPRIAEAPIHTRQ
                     TAIHPPFTPPADEPAPEPAAAAPPPPPPPPPPVLAPPVLAQGTNGAAAQGNSPGAVMA
                     PPVLAPPVVTATATTPAKSPLAAMLPDPSTVAGAPAVANRPAQRQAFRAYIELSQESG
                     TYLLRMLPSGETPPYGTYEAMVVLTDPDSDFFNVS"
     gene            complement(6856..7065)
                     /locus_tag="VFQ35_03405"
     CDS             complement(6856..7065)
                     /locus_tag="VFQ35_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HET9929702.1"
                     /translation="MTSEATLDPSRCPLCGNLNSCAVVEGSTEPCWCSAITIPRETLE
                     KIPLSAKDRACLCPRCAGLTQARSE"
     gene            complement(7192..>7675)
                     /locus_tag="VFQ35_03410"
     CDS             complement(7192..>7675)
                     /locus_tag="VFQ35_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929703.1"
                     /translation="SQLGVGSRFIVELCIVGDEAAESARSDELQSLPPAGLRVLVLDD
                     DLSVRTMLSRMVADVGGAAVAASRGEEAISLFRTARDMGQGFDVVILDLTIVGGLGGK
                     DTLQRLLEIDPTVRAIASSGYASDPILASPSLYGFVGVLPKPYTRQALRTALRLALAS
                     "
BASE COUNT         1273 a         2702 c         2458 g         1242 t
ORIGIN      
        1 agtctcctcc ggcaccgtgc tccacgagga cacgttggat ccctggcgag tggcatcgtg
       61 gacgcgcgtc cgctcgggcc tcgatacggc aagggtgcga cccacaccgc agcgcgccgc
      121 tcggtcgagt cctgctgtca gctcgggcct cgacgtgccc gcgacgagcg caaccgacga
      181 agcgcacagc tggattcact cggacatcat cgatccgtgg gcagggcgag ccctccgcca
      241 atagccgcgc agcatcgccg tcactcggag gggtgagcgc ggcgcgccgc acacgttttt
      301 cgcgctgctc gcgcgccagg aactttcgcg ggaattgcgc gcccgaaccg ccggtttgaa
      361 gtaataccta ttgcggtttc gcggcgttcg tggtgcttcg cgggggggac gggtgtgcgc
      421 gggggtttgc cgcccttcag ttcgaaggct cctcatttct cgaaagagcg agcgcttcct
      481 ccggccagcg gagtacgcgc gtgatgctgg agagggctcc gttcacctcg cgcaacgcgc
      541 gggcgtagag tttgcgttcg aggtcggcac gcgtcgattc gcgcaagcgc cgcgagagcg
      601 gcgcgccggt cgcgtctccg cgcgcttgga ccttcgccga gcagagtacg ctgtcgttct
      661 ccagatcgta cacgacgagc tcggcgtcga agcggcctcc gctccactcg gagtgcaacg
      721 cgttcagtct gcgaaagtgg gtgggcgggg agaacacgcg aacgacgagc tcggctagat
      781 agcggcgctc gctgagcgac tcgagcgctt cgtcctcggc tttggttcgt cgaggcgagt
      841 cccgaaatcg ggcgagcgga tccgccccca acgtctgcag gagctcgagc ggcatgatgt
      901 tctttcggtc gagccggtcg tcgcgcagcc gcacgagcac cgagctctgc tcgctctgcc
      961 cgtgggcgag cgtctcgtcg ggacaacgct gatgctcttg gcgcggccgc tcgggaagct
     1021 gacgatgcaa gcgcccctcg gcgcgggaga acagcccatc gggcgcgggc gcggcggcga
     1081 cctctgcttt gggtccaacc acgcgcaacg catcgcgccg acgcggctca caggcgagca
     1141 acgcgagccc ggcaacgatg aaaaaaaacc ggcgcgacat ggttcccacc cgcgttacct
     1201 cgtcgcgccg aaaattgaga aatttgtggt cactgaaggc gctcggcgcg cctcttcgaa
     1261 aaagagcgtc tctggggggt ccgaagcatt actcccaccc ccgcagtgcc agcctatcgc
     1321 gcagacccca ccccgaaccc ctaatcccca cccaaccgtg gtcccgaacc ttcccaaccc
     1381 tactgcgcga cccgaccctt ccttctgctt cgacccccca gagaccgctc gctcagactg
     1441 ccgctatcgg ccaccgaacc ccaccgggcg gtggccgaga ccgcaccgcg ccacgtccca
     1501 accacctcgt ggccccgcag cggtccgagc tcagcaaacc aaccttgcgc aacgcgccgg
     1561 cttcccgtgc tgggcaagcc tgagaaccgg aggtgcgcca ttcgctcacg aaggaccact
     1621 ttgcacgcga tgtgccagac ctcgcagtgg tgcccttcgg ggaagcgagg agccgcctaa
     1681 gcgcgtggtg aagtcgaaca cgctggtgca caagccactg aaatcaatgc aatttttctg
     1741 aggtcgcgag acgagactcg gcgcgccggt tccccctacg tccagaacct cagcccgagg
     1801 caggtgtatc tcgtccgtga tacaccccgt tggacagata cagtcaccgg gcagcttcgg
     1861 acgcctcgac ctccagcgct agatcgcccg ttaactaccc gtatttccga gagaactccc
     1921 aacccgctgg accgcagctt gtggtcccat gctcgcaacg ctaaccgctc aagcacacga
     1981 gcgcgcgctc actcgttgtt cggcggcttc accgtgaaca ccccctcatt tccacctgga
     2041 ccagacgcgg cgcgttggat tcgcggctgg cggctcgacc gactcattca cgtcggcaag
     2101 accgcttgtg tttatcgcgc gaccagcgag gccggtgtgg tcggggcggt caaggcgctc
     2161 cggcccgcgg ccatcgttca ccgcaccgcg gcagagcgct ttgctcgcga gatcgcggcg
     2221 ctgagcggcc tcacgcatcc ggccattccg cgattgctgg atcaaggcat cgacgacgaa
     2281 gggctcccct ggttcgtcac cgagtggatc gttggctggg acttggaaac gcaccgcgtt
     2341 cgcttgggtg gccgtttgct cgtcgaagaa gtgttcgacg taggcgttcg cctcctcgag
     2401 gcactggcgc acgcgcacgc ggtcggcttc atccaccgcg acgtcaagcc ggcaaacttg
     2461 ttcctcaccg aaaccggcga gctgaaggtg ctcgactttg gcctctgccg cggtctcgtc
     2521 gccgcatccg atttcggcat cactggcaac gtggtgatcg gaacccctgc cttcatggcg
     2581 cctgaacagg cgggccggcg ctgggcgcaa gtagacgcgc gtacggacat ttggtcggtt
     2641 ggagcaacct tgttcttgct cgcatcgggt cagcccgttc acctggggga ccccgagcag
     2701 cagcgtaagg cgtcgtatcg cgccgatgct cgctcgctag cgagcgtcga tgcgagcctc
     2761 ccattcggtc tcgttaccgt gatcgaccga gcgctgcgca gagatccgct cgagcgcttt
     2821 cagaacgcga cggagatgca cgaggcgctg ctcgaggcgc gcacgcccgg taagccaaag
     2881 ctcaggacgg cgccggtgat ggctagcacc gccaacgacc ctcatcgcca caccaccgag
     2941 caagatcgcg agctctacgc gccgagccag cgatttctga gcagcgcgct tcgcctcgtc
     3001 tcgagccccg accaaacgac gctccgcgag cgcaccctct cgcagcagag cgagctcttg
     3061 ataggtcgag acgccggtaa cagcggctgg tccatcggcg acacgcgcgt gtctcggctg
     3121 cacgcgagaa tcgcctggga cgatcgacaa ggctgttacc gcgccgcgga cctgcagagc
     3181 accaacggaa tgcgcgtgaa cggcctgcgc cgaacgacca cgacgctctc acacggcgac
     3241 gtggtccgcg tcggcgacag cgtgctcctc ttgaccgagc gcacgacgat gcaggacctc
     3301 gagcgcgctg ccgagcacgc cgcacgctcg accgcgaccc tcctgctcca tggcgagacg
     3361 ggcgtcggta aggaggtact ggcgcgccaa attcatgcgt ggagcgaacg tcgaggggcg
     3421 ttcgttgccg tcaactgcgg cgcgctcccg cgcgatctcg cggcgtcgga gctgttcggc
     3481 cacgcgaagg gcgcgttttc gggcgcggcc aacgcgaggc gcggagtctt tccggccgcg
     3541 cagggcggca cgctgctcct cgacgaagtg ggcgagcttc ctttggagct ccagccgctg
     3601 ctcttgcgag ctctgcaaga gcgcgcgatt cgccccgtcg gcgccgacat ggagatgccc
     3661 atcgacgtgc gcgttctcgc ggcgaccaac gtccagctcg agcaggcggt gaacgaggga
     3721 aagttccgcg ccgacctcta tgctcgcctc gcccagttcc cgatcaacat cccgccgctg
     3781 cgcgagcgcc gtgaacaaat cctcgacttg gcgcgagtct ttgcgcgggg cgctgggcac
     3841 gaacttacgc tggccgcgga cgcggccgaa gcgctcttgt tgtggcattg gccattcaac
     3901 gtgcgagagc tcgaaaatct catgcgccgc tggtgcatgc tggcgcgccc aggagccgtg
     3961 ctcggcctcg acttcttgca cagcgtgaac ccggcaatga cgagcacgct ccaagatcga
     4021 gcctctacgc cccccgccgc gtcgagcagc tcggggaccc agcgcaaccc gctgagcgac
     4081 cgcacccagc tcgagtcact gctgcacgcc tgcgacggca acgtttccga agttgcgcgg
     4141 cggctgaaca cgacgcgagc tcaagtctac cgatggctcg agcgctttgg catcgaaccg
     4201 tcgcgcgctc gcggcgacgg tggtcgccgg cgcggaaacg attgagcgcg gcgtacgccc
     4261 agctcgagcg ccgggcgtgg tatttcagga cccatgaagc agctgcaccg cgcggatctc
     4321 tacggctggt ctcggttcga cgaaagtaga aacatcgact ttcattcgct gttgtggcgc
     4381 cggcccgaag gcaacgtcgt catcgatccg ctaccgctca gtgagcatga acgaggccgg
     4441 ctccaggaat tcggcggcgt gaagcacgtc gtcatcacga attccgatca cgtccgcgcg
     4501 acggcagagc tcgtggcgct caccggcgct cgcacctatg gtccccgcgg cgaacgcggc
     4561 tcatttccgc tccaatgtga cgtgtggctc ggagacgagg atacggtggt cgaagggctg
     4621 gtcgcgctcg cgctcgaagg gtcgaagacc gacggggagc tcgcgctgtt gctcgaacgg
     4681 acgaccctca tcacgggcga tctagtgcgc gcgcacgctg gcggtcgcct cgactctctc
     4741 cccgaagcga agcttcgcga ccgcgcggcg gcacgacgtt cactggagcg cttggcgggg
     4801 atcgctagcg tcgttgcggt gttgccggga gacggttggc cgatgtttcg agatggacac
     4861 ggcgccctca ctgatctcgt ccgtcgcttc ggactcgaag cctagtcgcc tctcaccgag
     4921 agccgctccg ggttcccgcg cgttgcctct gcggttgcga tcacccctcc agagttgcca
     4981 caggtacaat ttcgcaacgg attgcgcggc cggggctact gctctcttga gtcgagctca
     5041 ttggcgacct gaaaagaatt ggccagtgtc gacgtaaggc aaaggcccaa gtgccggggg
     5101 gcgggtagcc cccgagcgaa atctcgcgaa gcggaccgtc gcctgaggcg acacgttccg
     5161 ctacgctgcg cgcgttggga ttctgagtgt gaggagacga ttcgaatgac catgcaggcg
     5221 ctttcgcttc cggagccgct ccctgacgac cccgaggtcg tcgcaacggc gctggagaca
     5281 gcagagattt tcggggtgca gggtgacgtt cgcgaagcgg tgcgctgggt gcgccgcgcc
     5341 gccgagctcg cgggcgatgc gggcaacgac gatcgcgcgc tcgtcctcgc gcgcgctgcc
     5401 gccgatctca ccgcgaccat gaacgatcgc gagagtgccg ccgcccctcc gccggctgcc
     5461 ccgccgcccc cgccgcaagc cgaagctagc cccgcggacg cggcgccggc cgaacccgcg
     5521 cgtgcggcac cacctcctcc gcctccaccg ccgcccccac cacctcctcc gccgccccca
     5581 ccgccaatca cggctgggtc gacgcgtccg tccacgcgcc ccgccagtga ctcacgcgcg
     5641 ccgcaggcgg tctcggtatc tccgatcgtg tcactcaact cggagagcga cacttcctca
     5701 tccgaagtgc gcgcacccgc gctcgatgcg aacggttcca acggcgcttc ggcgtcgccc
     5761 gagcctccgc gtgaagtcgt tgcggcgcag gcgagcggtt caccttcctc gagctcggcg
     5821 aagcccgctg tttcgcaccc acacgatggg ctcgacgatc tgttcgatga gccgcccact
     5881 ggggagcatc gcgcggcagc cgccgcagct ccggtcgcgc cagcaccagt cgccgcgagc
     5941 gttgcgctgg gcggcctccc gagtcttagt gcgacgaccg gcggcatcgg ccccgccggc
     6001 gctctcggca cgctgcagtc acacacggtg tcgcgcgagg cattggcaaa cgcggccacc
     6061 gtggaagcgc ccgtgttgcg accgctactg aaaccgccgt ccaagccgcc gcgtccagtc
     6121 acctcgaccg tgcttggagg agccgccgcg tccgtgtcgg caccgaatcc ggcgcccgtc
     6181 gcggtgccgc ccgcctctgc gccgcccatc gttggttccc cagtgattgc gccgccgctc
     6241 gcagcgccag tcgttgcgcc cccggtggcg gcgccggcgc ccatcgttgc ttccgccccc
     6301 gcgcccgagc caagcgcaga acgcgatgca cccccggtga ccgtcgcggc accgcgcatc
     6361 gcagaggcgc ccattcatac tcgtcaaacc gcgattcatc cgcccttcac gccccccgca
     6421 gacgagccgg cgcccgaacc cgcggctgcg gcgccaccac caccacctcc tccccccccg
     6481 ccggtgctcg ctccgccggt gctcgcgcaa ggaacgaacg gagccgcggc gcagggcaac
     6541 tctccgggag cggtgatggc tccgccggtg ctcgccccgc cggtagtcac ggcgactgcc
     6601 acgaccccgg cgaagagccc gctcgcggcc atgctgccgg atccttcgac ggtggctggc
     6661 gcgcccgcgg tcgcgaaccg ccccgctcag cgtcaagcgt ttcgcgcgta catcgagttg
     6721 agtcaggagt cgggaactta cttacttcgc atgttgccga gcggggagac tccgccgtac
     6781 ggcacttacg aagcgatggt cgttctcacg gatccagata gcgacttctt caacgtcagc
     6841 tgagaagcga ccgcttcatt cggatcgcgc ctgggtgagg cctgcacaac gcgggcacag
     6901 gcaggcgcga tccttggcgc tgagcggaat cttctcgagc gtttcgcgcg gaatcgtgat
     6961 ggcgctgcac caacacggtt cggtcgagcc ttcgaccacc gcgcaagagt tgaggttccc
     7021 gcagagcggg cagcgagagg gatcgagggt cgcttcggac gtcaccgacc aagcgttgca
     7081 cccttgctgg ctagggtcga agggtagcgg cgaagaccct agatttgccg gcgctgcgct
     7141 tagctgggcg ttgaagaacg ctctgcgtca ggcttaccca ttcgcgggcc ctcagctcgc
     7201 gagagcaagg cggagcgcgg tgcgaagcgc ttgccgcgtg tacggcttgg gcagaacccc
     7261 gacgaaaccg tagagcgaag ggctggcgag gattgggtcg ctcgcgtacc cactgctcgc
     7321 gatcgcccgc accgtcgggt cgatctccaa gaggcgctgg agcgtgtcct tcccgccgag
     7381 cccgccgacg atcgtcagat cgagaatcac gacgtcgaag ccctgtccca tatcgcgcgc
     7441 cgtccggaac agcgaaatcg cttcttctcc gcgcgaagcc gcgacggccg cgccaccgac
     7501 gtcagcgacc atccgcgaaa gcatcgtgcg gacgctcaag tcgtcgtcca aaaccaacac
     7561 acgtagaccc gctggtggta acgactgcag ctcgtcggag cgagcggact cagcggcttc
     7621 atcgccgacg atgcacagct cgacgatgaa gcgactgccc acgccaagct gcgac
//
LOCUS       DASTKS010000160         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1809261, whole genome shotgun sequence.
ACCESSION   DASTKS010000160 DASTKS010000000
VERSION     DASTKS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809261"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFQ35_03415"
     CDS             complement(<1..468)
                     /locus_tag="VFQ35_03415"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9929704.1"
                     /translation="MTEQVDVEGARMDAARLSEVVARFRRQHAEGVFPGGQLVVRRRG
                     RLVVDEAVGIARGFRETEDEPRTPFTTTQRSCLFSAGKPLVGIAIAVLEQARAIDVER
                     PVAFYFPEFAAAGKADITVLDVLLHRSGLYLREIERDFRRFGEWDSIIARIAST"
     gene            complement(537..1460)
                     /locus_tag="VFQ35_03420"
     CDS             complement(537..1460)
                     /locus_tag="VFQ35_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9929705.1"
                     /translation="MSLMRLRAAVHELHLDEHAWVETLFDEGRELLESGNGVFAYSYR
                     VRGGRGIELGHVAGRQSAPEFWRALSAWGADNQAALARCYSTGAALASFVIESAARAG
                     VAVTDFRAPFEAYGVGDLFTVVGHDRSGFGVFLTAPRAASGLKAFGNRRALERLAAEL
                     AAAMRLREQRRRAHVARLSASEELVARRLLEGASDKSIAGELGVSVSTVSTFARRLRK
                     KLGCRPGEEALLLSRARSGVQLRRRVVLFERLSAAECEVASELLVGRSYLEIARRREV
                     SLRTVASQCAAIFRKCGVSGQRELAAVLLGA"
     gene            2316..>2792
                     /locus_tag="VFQ35_03425"
     CDS             2316..>2792
                     /locus_tag="VFQ35_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9929706.1"
                     /translation="MNAHQFLVALTTVLTVAAITTVIFHRLRQPVVLGYILAGFIIGP
                     NVPVPLVADREVIQTLSEVGVILLMFSLGLEFSLSKLVKLGPVAALTAALQSGMMLWL
                     GFIVGRAFGWTNLESLFAGATVAISSTTIIAKTFDEQKVRGSLRELVVGILIVEDLI"
BASE COUNT          500 a          945 c          864 g          483 t
ORIGIN      
        1 cgtggatgcg atacgcgcaa tgatcgagtc ccattcgccg aagcggcgga agtcccgctc
       61 gatttcgcgt aagtagaggc ccgatcgatg cagaagcacg tcgaggacgg tgatatcggc
      121 tttgccagca gcggcgaact cggggaaata gaaggccacc ggccgttcca cgtcgatggc
      181 tcgcgcctgt tcgagaaccg cgatcgcgat cccaaccagg ggctttccgg cgctgaacag
      241 gcaagagcgc tgtgtggtcg tgaacggcgt ccgcggctcg tcctccgttt ccctgaagcc
      301 ccgagcgatt ccgaccgctt cgtccaccac gagtcgtccg cggcgccgca cgaccagctg
      361 accgccggga aacacgccct ctgcatgctg ccgccggaac ctcgctacca cttccgaaag
      421 ccgagccgcg tccatccgcg ccccttcgac gtcgacctgc tctgtcatgc gagggctagc
      481 atcgcgcgac tgcgcggctg cgcacatccg cattttcctg acgtcgagtc gcggtgtcag
      541 gcgccgagca agaccgccgc gagctccctt tgaccagaga cgccgcactt tcgaaagatg
      601 gccgcgcact gagaggcaac tgtccgcaac gaaacctcac ggcgtcgagc gatttccagg
      661 tacgagcgcc ctaccaggag ctcggaggcg acttcacact ccgcggcgct caaccgctcg
      721 aacagcacga ctcggcgccg aagctgaacg ccgcttcgtg cacgactcag gaggagcgcc
      781 tcttcgccag gccgacaacc gagcttcttt cgcagccgcc gcgcgaaagt cgagacggtc
      841 gagaccgaaa cgccgagctc gccggcgatg ctcttgtccg atgcgccttc gagcagccga
      901 cgcgctacca attcctccga agcactcaag cgggcaacgt gtgctcgccg acgctgctcg
      961 cgaaggcgca tcgctgccgc gagctccgct gccagccgct cgagcgcacg cctgttaccg
     1021 aaagccttca ggcccgaagc cgcgcgaggc gccgtcagaa aaacgccgaa cccagaccgg
     1081 tcgtggccga caaccgtgaa caggtcaccc accccgtacg cttcgaaggg tgcgcggaaa
     1141 tcggtaacgg cgacaccggc tctcgctgcc gattcgatga cgaaggaggc gagcgcagcg
     1201 ccggtcgagt agcagcgggc gagcgcagct tggttatcgg cgccccacgc cgacaatgct
     1261 cgccagaatt cgggcgcgct ttgccgcccc gcaacgtggc cgagctcgat gcccctgcca
     1321 ccacgaacgc gatacgaata tgcgaatacg ccgttgccgc tctcgagcaa ctcccgaccc
     1381 tcgtcgaaga gcgtctcgac ccaggcgtgc tcatccaagt ggagctcgtg aaccgccgcg
     1441 cggagccgca tcaagctcat cccctgaact ttacgcggtt cccatggagg tgagtggcga
     1501 aactgcgcgc agcgccagaa tcgctgtcgc gaagctccga aatggctcgg gagagcctcg
     1561 aacggactcg cgtgctcgaa tgatgaaacc gtgcgcacac cgagaaaccg cgttgcggag
     1621 gcgcccctca cgtgcttgca ttcaacgctc agtgatttcg cgcccgcgct ccgcacccgt
     1681 cttcgcgcca ctttcgtggc tgcagtggtg cgccgccttg ctgctcgtgc tggcggcttg
     1741 ggcagtgcgg ctttcgccgt tcccttattt tgcctccggt tacgagcccg cgacggtcgg
     1801 cgcaaaagtg ctgcgcagcg acgaagccgg attcgccagc agcgagaagt cggttcggct
     1861 cgatcagcgc gccgtcgcgc cgacagcgaa gccgaagcgc tcggctccct cgaagcggtt
     1921 tcacgagcca tcgtgcgcca ggctcgcctc atggggcggc agcgcgtcgg ccgaacggat
     1981 cgattccgtc tctgcgaacg ttcgcgaggc gaggcacgtc actccgattc ggaaacgcat
     2041 cccgcggctc ggcaccgacg aaccgccttg gtccgtgtag aaagctcgct gaaagagcgg
     2101 tcaacctagc ctcctgccgt ttcgatgcgg gagcgcgggc ggtgagactc gttcgaaggc
     2161 gaggcacgcg gtccggttgc atcacgtgca cgagcgcggg aacgctttcc gcatcgtgct
     2221 caccgcgcgc ggagcttcgg tcactgcacg cgccgcaggc tccgtctttt ccacgacttc
     2281 caacgtttcg aatacgacct cagtgggagg gtcgaatgaa cgcgcatcaa ttcctggtag
     2341 ctttgacgac cgtgctgacg gtcgcagcga tcacgaccgt catctttcat cggcttcgcc
     2401 agccagtcgt gctcggctac atcttggccg gcttcatcat cggcccgaac gtacccgtgc
     2461 cgctggtggc ggatcgggag gtgattcaaa cgctttcgga ggtcggcgtc attctgctga
     2521 tgttctcgct cggcctcgag ttcagcctga gcaagctcgt caagctcggt cccgtggccg
     2581 cgctgaccgc ggcgctgcag tcgggaatga tgctgtggct cgggttcatc gttggccgcg
     2641 cttttggttg gaccaacctg gagagcctgt tcgctggggc gacggtggcc atctcgagca
     2701 caacgatcat cgcgaagacc ttcgacgaac agaaagttcg cggctcgctg cgagagctcg
     2761 tcgtgggcat cctcatcgtc gaagatctga tc
//
LOCUS       DASTKS010000161         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_693921, whole genome shotgun sequence.
ACCESSION   DASTKS010000161 DASTKS010000000
VERSION     DASTKS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693921"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFQ35_03430"
     CDS             complement(<1..488)
                     /locus_tag="VFQ35_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9929707.1"
                     /translation="MGAISEYLASRVLAEHESFVVVSDVHLEQLGSKNTARALAELVR
                     DHPGREIVLGGDVFYLSHDSEDDDAVDSVLRILRAYPELEAALREHLAGGGRLTFIAG
                     NHDASLAEPRMQDALRRHLADAQASLSVARWFIRRGAVHVEHGHMWDPDNAPAHPLAG
                     WS"
     gene            695..2485
                     /locus_tag="VFQ35_03435"
     CDS             695..2485
                     /locus_tag="VFQ35_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9929708.1"
                     /translation="MRAYSERFPGGRITPPWAPGPLQRKTERTFPTLGWPRQTDSLCP
                     VCIREARSAVLGGNLDLRRFIDEHPGEIRANIVERDGKIVMQKSCPRHGDFEDVMSVD
                     PAFLKRIEQLFPGRDFEAPKHRLRDHGSSTIKYGRGSVLTVDLTNRCNMMCDPCFMDA
                     NQVGYVHELTWPDIKKILDDALDIKPRRQLSVQFSGGEPTLSPHFLEAVRYARKIGYF
                     CVQCASNGIRFAQEPEFCREAKAAGLRLCYLQFDGVTNEANSHRKVGNLFDVKLRAIE
                     NLAAAGIDVVLVVTVVKGVNDDQVGAIVRFAIENADKITVVSFQPVSFTGRDEEIGDE
                     ERHAKRYTLSHLAHDLHDQLGVTEPLRDWFPLSAMNPLSDVVDLMLGQSAEFGALKCG
                     CHPNCGIGTVLLVHKRTKKMVPVAEFLDIEQLLRDAQAVADAGQSRAQTVLNLALALL
                     RNFRPEKAPPGYGVSELFRQFLSQIGANGEKVGATEGDAAEFEWRFLFVAGMWFQDLY
                     NYDFRRTEMCIIPYGTQMGEISFCAYNTGAGFRQIIEKMKMTATVAEWFRTQGRHPVY
                     AKNQVLELPEGPLSVVVPEAHSRRRLPVLA"
     gene            complement(2498..3154)
                     /locus_tag="VFQ35_03440"
     CDS             complement(2498..3154)
                     /locus_tag="VFQ35_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929709.1"
                     /translation="MRSSSSTWSFAAITLMLGGCSSSTPSPAQSSPPQAEVRASNQRA
                     RVSKSAAAEDEAESVPSELAAMLEPRTCQPTLLNGTHFRELKDGKGRPHRAFLISPGE
                     ELCLIGSGGGDERNGSLSDLELSEDPVPLEKARERLISVELRTLEMGSVLVVKNHHRA
                     TLQFHGSMLVPHQLDLVSTSVCPVMPGRFSVEHWPDPLSSLLLTDFTLHDPSEQLGCE
                     "
     gene            3947..>4310
                     /locus_tag="VFQ35_03445"
     CDS             3947..>4310
                     /locus_tag="VFQ35_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9929710.1"
                     /translation="MTRREPIKSGRKPPQQPRSRATVSAILDAAIRVLDREGPDAATT
                     SRVAEVAGVSVGTLYQYFSNRDAIIDALQDREFVRATETINRVLLGGAASPVPDKQLA
                     QVVIEELLKLYRAAPALHR"
BASE COUNT          789 a         1359 c         1384 g          778 t
ORIGIN      
        1 tgagaccagc cagccagtgg gtgcgcgggg gcgttgtccg gatcccacat gtggccgtgc
       61 tcaacgtgca ccgcgccgcg tcgaatgaac cagcgcgcca cactgaggct cgcttgcgcg
      121 tccgctagat gccgacgcaa cgcatcctgc atgcgtggct cggccagcga cgcgtcatgg
      181 tttccagcga tgaacgtcaa ccgcccgccg cccgcgagat gctcgcgtag cgccgcttcg
      241 agctcaggat aggcgcgcaa tattcgcaag accgagtcga ccgcgtcgtc gtcctccgag
      301 tcgtgcgaga gataaaagac gtcgcctccg aggacgattt ctcggccggg atgatcgcgc
      361 acgagctcgg ccagcgctcg agcagtgttc ttgcttccta gctgctcgag atgaacatcc
      421 gaaacgacta cgaacgactc gtgctcggcc aagaccctgc ttgccagata ctccgagatc
      481 gctcccacgt cgaaggggcg agcagcgtcg ccggccggtg ggtcgattgt atgcgcggtg
      541 tgttgcagag ccgcttggca tcgcagtgag gtgaggaaga cctttggcgt ttctcgagta
      601 tgctagccgg ccaatgaacg aacttatccg ccgccgcgtg accgtgggcg cggaaacagc
      661 cttgcgaact ctggccgcgt gggcgtggcc cgttatgaga gcgtacagcg agcgcttccc
      721 cggcggacgc atcaccccgc cctgggcgcc ggggccgctt cagcggaaga ccgagcggac
      781 ctttccaacc ttgggctggc cgcggcagac cgactcgctc tgcccggtgt gcattcgcga
      841 agcgcgctcc gcggtactcg gcggaaacct ggatcttcgc cggttcatcg acgagcaccc
      901 gggagagatc cgcgcgaaca tcgtcgagcg tgacggcaag atcgtcatgc agaagtcctg
      961 ccctcggcac ggtgacttcg aagacgtgat gagcgtcgat cccgcgttcc tgaagcggat
     1021 cgagcagctg tttccggggc gcgacttcga agctcccaag caccgtctgc gcgatcacgg
     1081 ctcgtcgacc atcaagtacg ggcgcggttc cgtgctcacc gtggatctca ccaaccgctg
     1141 caacatgatg tgcgatccgt gcttcatgga cgcgaaccaa gttggttacg tccacgagct
     1201 cacgtggccc gacataaaga agattttgga tgacgcgctc gacatcaagc cgcgtcgtca
     1261 gctgagcgtg cagttctccg gcggcgagcc cacgctgagc ccgcactttc tcgaagccgt
     1321 gcgctacgcg aggaagatcg ggtacttttg tgtgcagtgc gcctcgaacg gcatccgctt
     1381 cgcgcaggag cctgagttct gtcgcgaagc gaaagcagcg ggcctgaggc tctgttacct
     1441 ccagttcgac ggcgtcacca acgaggcgaa ctcgcaccgc aaggtcggca atctgttcga
     1501 cgtaaagctc cgcgcgatcg agaacttggc ggcggctggt atcgacgtgg tcttggtcgt
     1561 cacggtcgtg aagggcgtga acgacgacca ggttggcgcg atcgtgcgct tcgcaatcga
     1621 aaacgcggac aagattacgg tcgtctcctt ccagccggtg agcttcacgg ggcgggacga
     1681 agagatcggc gacgaagagc gtcacgccaa gcggtacacg ctgagccatc tcgctcacga
     1741 cctccacgac cagctcggcg tgaccgaacc gctgcgagat tggttcccac tcagcgcgat
     1801 gaacccgctg agcgacgtgg tcgatctgat gctgggacag tccgcggagt tcggcgcctt
     1861 gaagtgcggg tgccatccta actgcggcat cggcaccgtg ctgctcgtgc acaagcgcac
     1921 gaagaagatg gttccggtcg ctgagttcct cgatatcgag cagctgctgc gcgacgcaca
     1981 ggcggtcgcc gacgcgggtc agagtcgtgc ccagaccgtg ctcaacctcg ctctcgctct
     2041 actcagaaac ttcaggcccg agaaggcccc gcccggctac ggcgttagcg agctatttcg
     2101 gcagtttttg agccaaatcg gcgcgaacgg cgaaaaggtc ggcgccaccg aaggcgacgc
     2161 cgccgagttc gagtggcgct tcctgttcgt cgccggcatg tggttccaag acctctacaa
     2221 ctacgatttt cggcgcaccg agatgtgcat catcccgtac ggcacgcaaa tgggagagat
     2281 ctcattctgc gcgtacaaca ccggcgcggg gtttcgtcaa atcatcgaga agatgaagat
     2341 gacggccacg gttgccgagt ggttccgcac tcaaggtcgg cacccagtct acgccaagaa
     2401 ccaagtgctc gagcttcccg aagggccgct cagcgtcgtc gttccggaag cgcattcgcg
     2461 aagacggctg ccggtgctcg cctgagcgcg tccccgctca ctcgcagccg agctgctcac
     2521 tgggatcgtg cagagtgaag tcagtgagca gcagggagct cagcggatct ggccaatgct
     2581 ccacgctgaa gcgtccgggc atgaccgggc acacgctggt gctcactagg tcgagctggt
     2641 gcggcaccaa catggagccg tggaactgca gggtcgcgcg gtggtgattc ttcaccacga
     2701 gcacggaccc catctccagc gtgcgtagtt cgacggaaat cagccgctct cgcgcttttt
     2761 cgagcggcac cgggtcctcc gagagctcga gatccgacaa agaaccgttg cgctcgtccc
     2821 cccctccgga gccgatgagg caaagctcct cgcccggcga aatcaagaag gcgcgatgcg
     2881 gtcggccctt tccgtctttg agttcgcgaa aatgggtgcc gttgaggagc gtcggctggc
     2941 acgtgcgtgg ctccagcatg gccgcgagct cggacgggac gctctccgcc tcgtcttcgg
     3001 cggcggcgct ctttgaaacg cgcgcacgct gattcgaagc gcggacctct gcttggggtg
     3061 gggagctttg ggcagggctc ggcgtcgagc tcgaacaacc accgagcatc aacgtgattg
     3121 ccgcgaacga ccacgtggac gaactggagc gcatcgtcga acattcagta tcgtacggag
     3181 gcgacaaaac ggaagcgcca aagtgcgcct acatcgtttt ggtgcgacga gatacgtccg
     3241 agcgcgacgg tagtcgccgc acgaggcgcc cgatgtaggc gctcatcctc catcttttcg
     3301 tcatcgaagc gcaacggagc gaagcgcggc gcgcggacgg atgctagagg ggcaggtcac
     3361 tccgcgctgc accgagccga aaacctttcg cgtgcgaggt ttgcttcgac gcttcgctcc
     3421 tcgagacgct tcggcctttc gtcgttgttc actcagcgcg cgcttctcgc ttcgcgttgc
     3481 tctgcactcg cgagtgtgtc gaacgcgacg gtcatcttgg ttgcgcggtg tggaacgagc
     3541 gcgacgacga tcgttgctcg taaggttcac gccaggctcg gctctgcgca cggcgcgagc
     3601 gaacgatgca ttggagaagt cgtggtctga cgcgcttgcg ccggcgttcg aaacgcgtcc
     3661 cgcagtcgca gcgacgagcg atcgagcgcg acgcgcgggc ggagcgccgt ggacgtggct
     3721 tcgacgcgcg gcgctcgtta aatacactgg tttcggccgt tgaaacgacg tgcgggcggc
     3781 gccgcagaga ccgatggacc gaggccgttg agcggcgatc tttgccgaaa cgaggctgct
     3841 tttcccgaat ccgtggtacg ttcgcgctcg agatgagcgc gattcctgct gaacagtctg
     3901 ttggctgccc tcgacgcctt ccactcgcgt aggattcgga tccaccgtga cacgccgcga
     3961 acccatcaaa tcaggcagaa agccgccgca acagccgcgc tcccgcgcga ccgtcagcgc
     4021 gattttggac gccgcgattc gggttctcga tcgtgagggg cccgacgccg ctaccacctc
     4081 tcgagtggcc gaggtcgccg gcgtcagtgt cggtacgctc tatcaatact tctcgaaccg
     4141 cgacgccatc atcgatgcct tgcaggatcg agagtttgtg cgcgcgacgg agacgatcaa
     4201 tcgagtcctg ctgggcggcg ccgcgagccc cgtgcccgac aagcagctcg ctcaggtcgt
     4261 gatcgaagag ctcttgaagc tctatcgagc cgcgccggcg ctgcaccgtc
//
LOCUS       DASTKS010000162         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1363218, whole genome shotgun sequence.
ACCESSION   DASTKS010000162 DASTKS010000000
VERSION     DASTKS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363218"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFQ35_03450"
     CDS             complement(<1..275)
                     /locus_tag="VFQ35_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929711.1"
                     /translation="MTTLLVMTAGQTDVQLVVNEQRHKLDGNTCGTLHDAVKERSWSV
                     VDAPTGRSRDLIKLLPEGQLPLCTPKLDAVLEHFGPSLPTSALILETT"
     gene            complement(272..2656)
                     /locus_tag="VFQ35_03455"
     CDS             complement(272..2656)
                     /locus_tag="VFQ35_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929712.1"
                     /translation="MPGKPDKQHRIRVKTLDGKPRDQSAINKLKSNQLGMPHLDIVGT
                     AVATELIICASKDTHIEQVRSKLGGAGLEAIDDGDTIIAATGAPRPAQPQPRQSGSAN
                     NAQPLPGKPYGFVALPKELKTSAPVWHDGTSSNGRLSGEIRFELGTLTPLLVGWERQP
                     VVAQDGEVPEWPIESQAEGDLASMNLNGRWSAPIAPGKSVLCPLRAPWGNRPVVIPGD
                     SLKGLLRHELGALLGAPMERVAERSYSYRPNQKFPYDQRDQRYRLGTASLEPRLARVR
                     TTTTTRIGDKEWPVPEQLDVLGMATRDQQAYFPRREGRGRDAVVIPPTREAEPYRGGL
                     GGGHALPDQILAEDARRKTIHTHIDVAQLQVEHRAVRPSAEVVTQYSATLRHLLDVNE
                     GHFSARHPHTGTDQQKQRTGQEAVEGGATRAFQPGDLVWVEWDTEKKCIVSLGWHYYY
                     RWAYTDSIRKTGGKLERHGLFPLDKEREKDGDAPKELSLVRRLLGYAFDKENPGVAGI
                     GKENTDHSQLMGRVFVNAGLEVVGDNDTAEQRFLDPTFLKELGMPRPSAVEYYLNQNL
                     DAQGRPQKRPSDNATLVTYGDAAGYDAPGELAGRKFYLDRQDAYRPHKTEDGKPPWED
                     ARETNRQNDRSTLALEASKPGRKFRFTVRFRDLDAAELAAIVVALSPDQFKGALGGTH
                     VDGYCSKLGYARPLGWGSVRIEAKQLLLLDEAGEGPTLNLESELAAWVTRNHRKTSTQ
                     DEWLAIHRRNHPNAADYPRPTQADKNGERHIYSFHTALRAEHSRNRRYKSGRSS"
     gene            complement(2656..>4430)
                     /locus_tag="VFQ35_03460"
     CDS             complement(2656..>4430)
                     /locus_tag="VFQ35_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929713.1"
                     /translation="LVGRGIKGAAVAMARRFFDPLPRSVSDDIKHGALRRSAWEFADA
                     RTAGVPRIRAGVGIRHKTGARAGGVLYEREVIPVGTTWQLAFRVDRSFALDDAEFTEA
                     EGILGYVLAEHWAKGRCWLGGGAARGLGWCHLDGLKAYRFDEAAYELWVKSGRTTLPA
                     AMATVPTVSPTRSWCFRTLDVNVSFGEYKPQPDEPAWGLDMFAVGPHDTERTLQPTGD
                     GQWAKPAWAISTQTPDALSTDRALLMAGDRPLLPGASVRGPIRHAFSRLERAAGHDVK
                     DPHLVEGDIGVDDLAGRAFGTVSKSSRILIRDARAEGDWVAAKLHMHAEDEFSAGSYG
                     SAMRDAVRVLKGTFPVRIVVESATLSEVEPLVEIVDREVALGALGHLPIGGHKTRGAG
                     AGHWQAKPWVVDDVAKARDWTALKEPTASATLPQRSFIERPDGADAWVKTTNGTITEP
                     LTLGAAAKIAKASLGEKLVAWWCDPTIDLDLHTPPATFGTSWPGEDADLKVDEIAFYA
                     QRAGWRAVRTSNGARFVFIQETAENEGERKARIVYTPARLHGFSQGRRPRFSSAQTGQ
                     NNVLLREWHVGSNVLGFTITREER"
BASE COUNT          665 a         1446 c         1488 g          831 t
ORIGIN      
        1 gtagtctcga ggatgagcgc cgacgttggc agtgaggggc cgaagtgctc gagtacggcg
       61 tcgagcttcg gcgtgcagag gggcagctgg ccttcgggta acagcttgat gaggtctcga
      121 ctcctgcccg tcggagcgtc gaccaccgac cacgagcgct ctttgacggc gtcgtgcagc
      181 gtgccgcagg tgttgccgtc gagcttgtgc cgctgttcgt tcacgacgag ctgcacgtcg
      241 gtttggcccg ccgtcatcac gagcaacgtc gtcacgatga ccttccgctc ttgtagcgac
      301 gattgcgcga gtgctcggcg cgaagggcgg tgtggaagga gtagatgtgc ctctcgccgt
      361 tcttgtcggc ctgtgtcgga cgcgggtagt ccgccgcgtt cggatggttg cgacgatgga
      421 tggcgagcca ctcgtcctgt gtcgatgtct tccggtgatt ccttgtcacc caggctgcga
      481 gctcagactc aagattcagc gttggcccct caccagcttc gtcgagcagc aggagctgct
      541 tcgcctcgat gcgcacgctg ccccagccga gagggcgcgc gtagcctagc ttcgagcagt
      601 agccgtcgac gtgcgtgccg ccgagcgcac ccttgaactg gtcggggctg agcgcaacga
      661 cgatcgccgc cagctcggcg gcgtcgagat cgcgaaagcg cacggtgaag cggaacttgc
      721 gcccaggttt cgacgcctcg agtgcgagcg tactgcggtc gttctgccgg ttggtctcac
      781 gagcatcctc ccaaggcggt ttcccgtcct cagtcttatg aggtcggtag gcgtcctggc
      841 ggtcgaggta gaacttgcgg cccgcgagct cgcctggcgc gtcgtagccg gcggcgtcgc
      901 cgtaggtcac gagcgttgcg ttgtccgacg gccgcttctg ggggcggccc tgagcgtcaa
      961 ggttctgatt aagatagtac tccaccgcac tcggccgggg catgccgagc tccttgagga
     1021 aggtcgggtc gaggaaacgc tgttcggcgg tgtcgttgtc gccgacgacc tcgagtcctg
     1081 cgttgacgaa gacgcggccc atgagctggg agtggtcggt attctccttt ccgatccctg
     1141 caacgcccgg gttctccttg tcgaacgcat atccaaggag ccgccgtacc aacgagagct
     1201 ccttcggcgc gtcaccgtcc ttctcgcgct ccttgtcgag cgggaagagt ccatgccgtt
     1261 cgagcttgcc acccgtcttg cgaatcgagt cggtgtaggc ccagcggtag tagtagtgcc
     1321 agccgagcga gacgatgcat ttcttctcgg tgtcccactc gacccagacg agatcgccgg
     1381 gttggaaggc gcgcgtagcg cctccttcaa cggcttcttg ccccgtgcgt tgcttctgct
     1441 ggtccgtgcc ggtgtgcgga tgtcgcgcgc tgaagtggcc ttcgttcaca tcgagcaagt
     1501 gtcgaagcgt cgcagagtac tgggttacga cctcagccga agggcgcacc gctcggtgtt
     1561 cgacctggag ctgggcaacg tcgatgtgcg tgtgaatcgt cttgcgtcga gcatcctcag
     1621 ccaagatctg gtccggcaat gcgtggccac ccccgagccc gccacgataa ggctcagcct
     1681 ctcgcgttgg aggaatgaca acagcgtcgc gcccccgacc ctcacgacga gggaagtagg
     1741 cttgctgatc tcgcgtcgcc attccgagaa catctagctg ctcagggacc ggccactcct
     1801 tgtctccaat tctcgtagtc gtggtcgtgc gtacgcgcgc aagtcgcggt tccagagaag
     1861 cggtgcccag tcgatagcgt tggtcgcgtt ggtcgtatgg aaacttctgg ttcggcctgt
     1921 acgagtacga gcgctccgcg acgcgctcca tcggcgcgcc aagcagcgcg cccagttcgt
     1981 ggcgcaggag gcccttcagc gagtcgccag ggatgacgac ggggcgattg ccccacggcg
     2041 cgcgcagcgg gcagagcacg gacttgcccg gcgcgatcgg cgcgctccag cgaccgttga
     2101 ggttcatgct agcgaggtca ccttcggcct gggactcgat cggccactct ggtacctcgc
     2161 catcctgggc cacaacgggc tgccgctccc agccgacgag gagcggcgtg agagtcccga
     2221 gctcgaagcg gatctctccc gagaggcgcc cgttgctgct cgtgccgtcg tgccagacag
     2281 gcgcggaggt cttcagctct ttcgggagtg cgacgaagcc gtacggcttg cccgggagtg
     2341 gctgcgcgtt gtttgccgat cctgactgtc gaggttgcgg ctgcgccggt ctcggggcgc
     2401 ccgtcgccgc gatgatcgta tcgccatcgt cgatcgcttc gagcccagct cctccaagct
     2461 tcgaacgtac ctgctcgatg tgcgtgtcct tcgacgcgca aatgatgagc tcggtggcga
     2521 cggcggtgcc gacgatgtcg agatgcggca tgccaagctg gttgctcttc aacttgttga
     2581 tcgcgctctg atcgcgcggc ttcccgtcga gcgtcttgac gcggatgcgg tgctgcttgt
     2641 cgggtttgcc gggcatcagc gttcctcccg agtgatggtg aaaccgagta cgttgctgcc
     2701 cacgtgccat tcgcgcagga gcacgttgtt ctgcccggtc tgggctgagg agaagcgcgg
     2761 gcgtcgtccc tgagaaaaac cgtgcaaacg tgcaggcgtg tagactatgc gggcctttct
     2821 ttcgccctcg ttctcggccg tttcttggat gaatacgaag cgcgcgccgt tcgacgtacg
     2881 cacggctcgc cagcccgcgc gctgggcgta gaacgcgatc tcgtcgacct tgaggtcggc
     2941 gtcttcacct ggccacgagg tgccgaaggt cgcgggcggt gtatgcaagt cgaggtcgat
     3001 ggtcgggtcg caccaccacg cgacgagctt ctcgccgagg gaggctttcg cgatcttcgc
     3061 ggctgcacca agcgtgagcg gctccgtgat cgtgccgttc gttgtcttca cccaggcatc
     3121 ggcaccgtca ggccgttcga tgaaggagcg ctgcggcagc gtggcgctgg ccgtcggctc
     3181 cttcagtgcc gtccagtccc gcgccttcgc gacgtcatcg acgacccagg gcttcgcctg
     3241 ccagtggccc gcgcctgcgc cgcgcgtctt gtggccgccg atcggaaggt gcccaagcgc
     3301 accaagcgcg acctcgcgat cgacgatctc taccagcggc tcgacctcgc tcagcgtcgc
     3361 gctctcgacg acgatgcgca ccggaaacgt tcccttgagc acgcgcaccg cgtcgcgcat
     3421 ggcgctccca taggagcccg ccgagaactc gtcctcggcg tgcatgtgca gcttcgcggc
     3481 gacccaatcg ccctcggcgc gcgcgtcgcg aatgaggatg cggctactct tgctgacggt
     3541 accaaaagct cgcccagcga ggtcgtctac gccgatatcg ccctccacga gatgcgggtc
     3601 cttcacgtcg tgacctgccg cgcgctccag gcgcgagaag gcgtggcgaa tcggaccgcg
     3661 cacgctcgcg ccggggagca gagggcggtc gcccgccatc agcagcgcgc ggtcggtgct
     3721 cagtgcgtcg ggtgtctgcg tgcttatcgc ccacgcgggc ttggcccatt ggccatctcc
     3781 ggtcggctgc aaggtgcgct cggtgtcgtg cgggccgacg gcgaacatgt cgaggcccca
     3841 cgcgggctca tcgggctggg gcttgtactc gccgaacgag acgttcacgt ccagcgtgcg
     3901 gaagcaccac gagcgtgttg gcgagacggt cggcacggta gccattgcgg cgggcaacgt
     3961 cgtgcggccc gacttcaccc acagctcgta ggccgcctcg tcgaagcggt acgccttcag
     4021 cccgtcgagg tgacaccagc caagaccacg cgcggccccg cccccgagcc agcagcgccc
     4081 cttggcccaa tgctcggcga gcacgtagcc gagaatgccc tcggcctcgg tgaactcggc
     4141 atcgtcgagt gcgaaggagc ggtcgacgcg aaaggcgagc tgccacgtcg tgcctacggg
     4201 gatgacctcg cgctcgtaga gcacgccgcc tgcacgggct cccgtcttgt gacggatgcc
     4261 gacacctgca cggatgcgcg gcacgcccgc cgtcctcgcg tcggcgaact cccaggccga
     4321 gcggcgcagc gcaccgtgct tgatgtcgtc cgacacggag cgcggcagcg ggtcgaagaa
     4381 gcgcctggcc atcgcgacgg ctgcgccctt gatgccgcgc cctacgagcg
//
LOCUS       DASTKS010000163         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_916382, whole genome shotgun sequence.
ACCESSION   DASTKS010000163 DASTKS010000000
VERSION     DASTKS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916382"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..661)
                     /locus_tag="VFQ35_03465"
     CDS             complement(203..661)
                     /locus_tag="VFQ35_03465"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9929714.1"
                     /translation="MSVEARLHSLGIVLPEASNPAANYANYVRTGNLLFMAGKGPLPV
                     DGKLPKGKLGSAFSTQEGYAFARSVGLDILAAVRSAVGDLDRVARVVKLQGFVNATPE
                     FEEHAQVLNGCSDLMVEVFGPKGVHARSVFGAASLRANLPIVIDSIFEVA"
     gene            complement(714..1853)
                     /locus_tag="VFQ35_03470"
     CDS             complement(714..1853)
                     /locus_tag="VFQ35_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9929715.1"
                     /translation="MKWSKLLPFGSLACLLASSCSSEPTIEGFEVRAATFNAALAPAF
                     APLVSERTEPVLGALTASSSSLDLLCVQEFWLEQHWQALAASVANELPHTLRLPPRPG
                     SGTCTPEELGALGQCLQAACPDTTGENLVACATTSCAAEVTALRPGCLSCIMNHISED
                     FSTCVGPEVAADPAIYGGAFDVGMLSRLPIAEHDSKELSSYFVRAAVLYAKLEVPGAG
                     PVHAFCTHLGSPLDIVPYGGSYGSWHDEQREQVKELLAFIHEKTQDKGRVLVLGDMNN
                     GPAHQSIQGEWADNYAALTAEGLQDRYLLQTDAACTWCDDNSYHPGATPTLVDHILER
                     GFEDASATISRTYVDPVTFNLEDGSITANLSDHYGLRGVIKREAR"
     gene            1991..3463
                     /locus_tag="VFQ35_03475"
     CDS             1991..3463
                     /locus_tag="VFQ35_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929716.1"
                     /translation="MDAHGFISVIACAGHLGFARLAWSRRGKSPVAPWLGLLFLDTFV
                     WNFADLAFALSSDPLWHTIDRVFSSLMPAFALHVVVAFVGRARSLRELIALVYVAFFA
                     VALLSGFEVWWQLLLAGGSVTMLLALVLLVRYRQSLSEPVERARTDLIGWAIALGTVF
                     SSTDLWFNEIAGFTAPRLGNLATLIAMALFAVATLRLGLLGGKVPQLLLVYAVFAGAL
                     AVVAGVAAVNLLENSWAIGLVSGSALVLVLTASARELGRHAALNRERTRRLSLLGRLS
                     EQLAHDLKNPLAALKGALQFLEAERAAGRSLDAHAEFLQLMLDQVERVGRTVSEYQRL
                     AKVEPVLRRASLNQVVEGVLALGRFATSPNIEVKSELSPSLPECQLDPELIAAALDNL
                     LRNACEALLEGGVVTVRSERVRDHLALRVEDSGVGMHAREIERATDEFYTTKASGTGL
                     GLSFAQRVARAHGGNLELSSEPGKGTSVTIFIPCDQSSSL"
     gene            3466..>4653
                     /locus_tag="VFQ35_03480"
     CDS             3466..>4653
                     /locus_tag="VFQ35_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9929717.1"
                     /translation="MERERERERERVLVVDDDAAVRQVLAALLTQAGFEVLRAGSGDE
                     ALRVLERQPVDVIVSDVRMPGLDGMALLARVRREWPEIPVILLTAHASVTLAVEAMKA
                     GAHDFVEKPFEREGVLFVVRKALRATSAGRDAPPRNESSVDLLGVTEALDEVRERIRR
                     AAASNATVLIRGETGTGKELVARSIHTQSARRGGPFVALNCAAVPENLLESELFGYEK
                     GAFTGAASRKPGRVELSEGGTLFLDEVGDVPLSTQVKLLRVLQERAIERLGGTASVKV
                     DVRFLAATHRNLEDLMAQGKFREDFFYRLNVIPIEVPPLRERSRDIEALARHFCQIAS
                     RANNKTLAELEPGVSQLLASQPWPGNVRQLENFVERLVVLSDGPTITLADAQRELARE
                     SARR"
BASE COUNT          776 a         1533 c         1496 g          848 t
ORIGIN      
        1 gaggtcatag gcgcgataaa caatgccgac gccgccgcgg cccacctcgt gctcaaccac
       61 gaagcggccg cccagcttgt gtccggaagg cgcgataatc gacactccca gcgcttttac
      121 tccgcgcgct cgacggcggc gaggggctcg tcaacggagg ggcactccgg ccggcggcct
      181 cggggccggc gcagcccagc cctcaagcga cttcgaagat cgaatcgatg acgatgggaa
      241 ggttcgcgcg cagcgacgcg gctccgaaaa ccgagcgcgc atgcacgcct ttgggcccga
      301 acacttcgac catcagatcc gagcagccgt tcagcacttg cgcgtgctcc tcgaattcgg
      361 gggtcgcgtt cacgaagccc tgcaacttga ccacccgcgc cacgcgatcg aggtcgccta
      421 ccgcggagcg gaccgccgcg aggatatcca aacccaccga gcgagcaaaa gcgtagccct
      481 cttgagtgga gaaggcgctc cccaacttgc ctttcggaag cttaccgtcg acggggagcg
      541 gccccttgcc ggccatgaag agtaggttcc cggtgcgcac gtagttcgca tagttcgcag
      601 caggattgct ggcctctggc agcacgatgc cgagcgaatg caggcgagcc tcgacggaca
      661 tgcgcgagaa catggtgagg tgcgggccac gcgtcaatcg gcccgaggct acgttagcgc
      721 gcttctcgct tgatcacgcc gcggaggccg tagtggtccg agaggttcgc ggtgatcgag
      781 ccgtcttcga gattgaacgt gaccgggtcg acgtacgtgc gtgagatcgt cgcgctcgca
      841 tcttcgaatc cccgctccag tatgtgatcg accagcgtcg gagtggcccc tggatggtag
      901 ctgttgtcgt cacaccaggt gcaagccgcg tcggtttgca ggagatagcg gtcctggagg
      961 ccttctgcgg tcagcgcggc gtagttgtcg gcccattcac cttgaatcga ctggtgggcg
     1021 ggaccgttgt tcatgtcgcc gagcacgagg actcggccct tgtcttgcgt cttttcgtgg
     1081 atgaaggcga ggagctcctt tacttgctcg cgttgctcgt cgtgccatga gccgtaactg
     1141 ccgccgtacg gcacgatgtc gagtggtgag cccaagtgag tgcagaaggc atgcacgggc
     1201 cccgcgccag gaacctcgag cttcgcgtac agcaccgcgg cgcgaacgaa atacgacgag
     1261 agctctttgc tgtcgtgttc ggcgatgggc aagcggctca gcataccgac gtcgaaggcg
     1321 ccgccgtaaa tcgctggatc agccgccacc tcgggcccca cacaggtcga gaagtcctcg
     1381 ctgatgtggt tcatgatgca gctcaggcag cccggacgga gcgccgtgac ttctgccgcg
     1441 cagctcgtgg ttgcgcacgc gacgaggttc tcgccggtcg tgtcggggca cgctgcttgt
     1501 agacattgcc cgagcgctcc gagctcttcg ggcgtgcaag tgccgctccc agggcgcggc
     1561 ggaaggcgca gagtgtgcgg cagttcgttt gcgacgctcg ccgcgagcgc ttgccagtgc
     1621 tgttcgagcc agaactcctg cacgcagagc aaatcgagag agctcgagct cgccgtgagc
     1681 gctccgagta cgggttcggt ccgctcgctc acgaggggcg cgaatgccgg cgcgagcgcg
     1741 gcgttgaagg tcgcggcccg cacctcgaag ccttcgatgg tcggctcgct ggagcacgag
     1801 gacgcgagga gacacgcgag cgaaccgaac gggagcagct tggaccactt catgttcgct
     1861 ccgagagcaa cccgcgtgcc attccgcgat tggcgtcgga atcgctgttc gcgaagtcac
     1921 gagcgccgcc cgttgtgcag gattctgcac agcgtgcgct tgcacatgcc cgctatactc
     1981 gactgaaccg atggacgccc atggcttcat cagcgtgatc gcctgcgccg gtcacctggg
     2041 ttttgctcgg ctcgcctggt cgcggcgagg gaagagccct gtcgccccgt ggctcgggtt
     2101 gttgttcctc gatacgttcg tttggaattt cgcagacctc gcgtttgcgc tgagtagcga
     2161 cccgctttgg cacaccatcg atcgggtctt ctcgagcttg atgccggcgt ttgctctgca
     2221 cgtcgtggtc gccttcgttg gtcgagcgcg ctcactgcgg gagttgatcg ccctcgtgta
     2281 cgtggcgttt ttcgcggttg cactgctttc agggttcgag gtttggtggc agctgctgct
     2341 cgcgggcggc agcgtaacga tgttgctggc gctggtgctt ctggttcgct accgccagtc
     2401 actctcggag ccagtcgaac gggcgcgcac ggatctcatc ggttgggcca tcgcgctcgg
     2461 cacggtgttc agttctaccg atctctggtt caacgaaatc gccggattca ccgctcctcg
     2521 gctagggaac ctggcgaccc tgatcgcgat ggcgctattc gccgtggcga cgctgcgact
     2581 cggtttgctc ggtggaaagg tcccgcaact cttgctggtg tatgcagtgt ttgctggcgc
     2641 ccttgcggtc gtggcgggcg ttgcggcggt gaacttgctc gagaactcgt gggccatcgg
     2701 tctcgtctcg ggttcggctt tggtgttggt gctcaccgcg tcggcgcgcg agctcggtcg
     2761 ccatgccgcc ttgaatcgcg aacgcacccg ccgcctctcc ttgctcgggc ggctctccga
     2821 gcagctcgct cacgacctga agaatccgct ggccgcgttg aagggagcgc ttcagttctt
     2881 ggaagccgaa cgcgccgcgg gtcgctcgct cgacgcacac gccgagttcc tgcagctcat
     2941 gctggaccag gtcgagcgcg tcggccgaac cgtgagcgag taccagcgtc ttgccaaagt
     3001 cgaaccggtg cttcgccgcg cgtcgctcaa tcaggtcgtc gaaggggtcc tcgcccttgg
     3061 tcgctttgcc acgagtccca acatcgaagt gaagagcgaa ctgtcgccct cactgcccga
     3121 gtgccagctc gaccctgagc tcatcgcggc ggcactcgac aacctgctcc gcaacgcttg
     3181 cgaagcactg ctcgagggcg gcgtcgtcac cgtccggagc gaacgtgttc gcgaccacct
     3241 agcgctccga gtcgaggata gcggggtcgg catgcatgcc cgcgaaatcg agcgcgcgac
     3301 tgacgaattc tataccacca aggcaagcgg caccgggttg ggcctgagct tcgcgcagcg
     3361 agttgcgcgc gctcacgggg gcaacctcga actttcgagc gagcccggca agggtacgag
     3421 cgtcaccatc ttcattccgt gcgaccagtc gagtagcctc tagacatgga acgggaacgg
     3481 gaacgggagc gggaacgggt actggtggtg gatgatgacg ccgcggtcag gcaggtgctt
     3541 gcggcgctcc tcactcaggc cggcttcgag gtcctgcggg cgggctcggg ggacgaggcc
     3601 ttgcgagtgc tcgaacgaca accggtcgac gtcatcgtca gcgacgtgcg catgcccggt
     3661 ctcgatggca tggcgctgct cgcgcgggtg cgccgcgagt ggccggagat tcccgtaatt
     3721 ctcctcaccg cgcacgcttc ggtcacgctc gcggtcgaag cgatgaaagc cggcgcccac
     3781 gacttcgtcg aaaagccttt cgagcgcgag ggcgtgctct tcgtcgtgcg aaaggctctt
     3841 cgcgcgacgt ctgcaggaag agacgcgccg ccgcgcaacg aatctagcgt ggatctgctc
     3901 ggcgtcacgg aggcgctcga cgaagtgcgc gaacgcatcc gtcgcgccgc cgccagcaac
     3961 gccaccgtgc tcattcgcgg cgagaccggc acgggcaaag agctcgtagc gcgctccatc
     4021 cacactcaga gcgcgcgccg cggtggcccc ttcgtcgcgc tcaactgcgc ggcagtgccc
     4081 gaaaacctgc tcgagagcga gctctttggc tacgaaaagg gcgccttcac cggcgccgcc
     4141 tcgcgcaagc cgggccgcgt cgaactcagc gagggtggca ccctgttcct cgacgaagtc
     4201 ggcgacgtcc ccctctccac gcaggtcaag ctcttgcgcg tcttacagga acgcgccatc
     4261 gaacgcctcg gaggcaccgc cagcgtcaaa gtcgacgtcc gcttcctcgc cgccacccat
     4321 cggaacctcg aagacctcat ggcccaaggc aagttccgcg aggacttctt ctaccgcctc
     4381 aacgtcatcc ccatcgaagt tccacccctc cgcgagcgct cgcgcgacat cgaagccctc
     4441 gctcgccact tttgccaaat cgcgtcccga gccaacaaca aaaccctcgc cgaactcgaa
     4501 cccggcgtct ctcaactgct cgcctcccaa ccctggccag gcaacgttcg gcaactcgaa
     4561 aacttcgtcg agcgcctcgt cgtcctcagt gacggcccca ccatcaccct cgctgacgcc
     4621 caacgcgaac tcgcgcgcga atccgcccgc cgt
//
LOCUS       DASTKS010000164         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1586697, whole genome shotgun sequence.
ACCESSION   DASTKS010000164 DASTKS010000000
VERSION     DASTKS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586697"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VFQ35_03485"
     CDS             <1..422
                     /locus_tag="VFQ35_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929718.1"
                     /translation="LPEATLALARYVRLLELHMRHEEELLLPIYARETPSKRWPVVLY
                     TGQHQRMRELLASTTERVAALARREDVRGRDIIELLDHERTYKHLAEHHDGAEREGFF
                     PALDKLTTASECSELIESCWKEWLAAELTSPELGSRD"
     gene            complement(525..2309)
                     /locus_tag="VFQ35_03490"
     CDS             complement(525..2309)
                     /locus_tag="VFQ35_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9929719.1"
                     /translation="MRRHRFTLFGWAMAALVAGCGSDKEKNDGAGGSTSAGGDAATGL
                     GGLAGASNAMGGTVNGHGGASSGHGGGTNAAGGAVTSASGGAGAAGGPTAAVCTKAPG
                     GGSSTVQKPELIKTLFDRWHEAWLGSPAVADLDGDGVNEIVVPRHDLLMIWHLDGTLV
                     KKQQLPGRIWAPPVVADLVPSRPGLEVAVASRQQIAAWDAAGNALPGFPVTFRDEMRS
                     LAAEDIDGDGSLELVAVTTSDLKQGGRTDILIAYHNDGSVVAGFPPNTTGASGCDDAC
                     YVHAGFDQNIALGDVDGDGIADLLAPQDNAYVSLHHGTGVAFNAASIFRGRTKFLGIR
                     FLLDYALAQQGYADDEEASNQAHFTDTAPAIVDVDGDGKNELVMVSSVQNAAQTDRER
                     GVALWVVRNDGTRPAAWVKPFHAPNFIDGLWDPGDNIVATTNQVTVADIDAASRGFEF
                     IFAGFDGKLHAVSSDNRELWSYSFTTSKAVLTGGVVAADLSGDGIPELVFNTYSTAAD
                     ASALYVLGADGKLLHRVALPDRGAMPVPTIADVDGDGDLEIVVSLKDGVDKMRQVFVY
                     SVAGSSTNCMPWPTGRGNLRRTGFVASP"
     gene            complement(2475..3062)
                     /locus_tag="VFQ35_03495"
     CDS             complement(2475..3062)
                     /locus_tag="VFQ35_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9929720.1"
                     /translation="MPTDAPNPPSVHPPPRVFDELWKARTLLLFGEITMQLAEVVSAQ
                     ILALASASDAPIRLLINSPGGHVEAGDTIHDVIRFVDARVVAVGTGWVASAAALVYVA
                     APRERRLSLPNTRFLLHEPRGGASGPASDVEIEASQVLSMRARLNRIFAEATGQPIEK
                     IARETQRNHWMNAEAARDYGLVGRIVSRVGELGID"
     gene            3186..3716
                     /locus_tag="VFQ35_03500"
     CDS             3186..3716
                     /locus_tag="VFQ35_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9929721.1"
                     /translation="MKKSDSRVRTVHLGEARILLSSPESILLVIGTGCILRTAAGTQT
                     GLPSGTLLVREHQERRPFDGAVATAIELMKQKLEQPLRVSALAKAVGVSRAAFARRFV
                     RATGFSPLRFLHTLRLERAAQLLVETDLTLSDIASRVGYVSEFAFSRAFKRHRGLPPS
                     VFRRSSAAPRAMALAA"
BASE COUNT          689 a         1364 c         1276 g          660 t
ORIGIN      
        1 ggttgccgga agcgacgctg gcgctggcca ggtacgtgcg cctgctcgag cttcacatgc
       61 ggcacgaaga agagctgctg cttcccattt acgcgcgcga gacaccgtcc aagcgctggc
      121 cggtggtgct ctacaccggc cagcaccagc gtatgcgcga gctcctcgcg tcgaccaccg
      181 agcgagtagc cgccctagct cgacgagagg acgtgcgagg gcgagacatc atcgagctct
      241 tggaccacga gcgcacctac aagcacctcg ccgagcacca cgacggcgcc gaacgagaag
      301 gtttcttccc cgccctcgac aagctcacga ccgcgagcga atgctcggag ctcatcgaga
      361 gctgttggaa ggaatggctc gcggcagagc ttacgtcccc tgagctcggc tcgcgcgatt
      421 gagtagcgcg attcgaggcg cttgcgggcc cgcgcaatgc tgggtcattc gcgagcgagc
      481 cgagaacgca gattacccct tagctcaggg gctcgcgacg aagctcaggg gctcgcgacg
      541 aagcccgtgc gccgcaagtt tcctcgcccc gtcggccatg gcatgcaatt ggtcgaagag
      601 cccgccacgg agtacacgaa cacttgacgc atcttgtcga cgccgtcctt caggctgacc
      661 acgatctcca agtctccgtc tccgtccacg tcggcaatgg tcggtaccgg catcgcgccg
      721 cgatccggca acgcgacccg gtgtagcaac ttgccgtccg cgccgagcac atagagcgcg
      781 ctcgcgtcgg cggcggtcga gtaggtgttg aacacgagct ccggaatgcc gtcgcccgag
      841 agatctgccg cgaccacgcc gccggtcaac accgctttgc tggtcgtgaa cgagtaactc
      901 cagagctcgc gattgtcgga gctcacggcg tgcagcttgc cgtcgaagcc cgcgaaaatg
      961 aactcgaagc ctcgagaggc cgcgtcgata tcggcgacgg tgacttggtt cgtggttgca
     1021 acgatgttgt cgcccgggtc ccacaagccg tcgatgaagt tgggcgcgtg aaaaggcttt
     1081 acccacgcgg ccgggcgcgt cccgtcgttg cgcacgaccc acagcgcgac gcctcgttcg
     1141 cgatccgttt gggccgcgtt ttgaaccgag ctcaccatca cgagctcgtt tttgccgtcg
     1201 ccatcgacgt cgacgattgc gggcgcggtg tcggtgaagt gcgcttgatt ggaagcctct
     1261 tcgtcgtcgg cgtagccctg ctgagcgagc gcgtagtcga gcaagaagcg aatccccaag
     1321 aacttggtgc ggccccgaaa aatcgaggcg gcgttgaacg ctacaccggt gccatggtga
     1381 agactcacgt aggcgttgtc ctgcggcgcg agcaagtcgg cgatgccgtc gccgtctacg
     1441 tctccgagag cgatgttctg gtcgaacccg gcgtgaacgt aacacgcgtc gtcgcaaccg
     1501 ctcgccccgg tggtgttcgg agggaagccg gcaacgacgc ttccgtcgtt gtgataggcg
     1561 atgagaatat cggtgcggcc gccttgtttc agatcgctgg tcgtcaccgc gacgagctcg
     1621 agcgagccgt ccccgtcgat gtcctcggcc gcgagcgaac gcatttcatc gcggaacgtg
     1681 acggggaagc cgggtagcgc gttgccggcg gcgtcccagg ccgcaatctg ttggcgcgaa
     1741 gcgaccgcga cttcgagacc ggggcgcgac ggcaccaaat cagccacgac cggcggcgcc
     1801 cagatgcgac cggggagctg ctgcttcttc accaaggtgc cgtccaggtg ccaaatcatc
     1861 aagaggtcgt ggcgcggcac cacgatttcg ttcactccgt cgccgtcgag atcggctact
     1921 gcaggtgagc ccaaccaggc ttcgtgccag cggtcgaaca acgtcttgat gagctccggc
     1981 ttttgcaccg tgctcgagcc gcccccggga gctttcgtgc agaccgccgc cgtggggccc
     2041 cccgccgcgc ccgcgccgcc agacgcgctc gtgacggctc cgcccgccgc gttcgtgccg
     2101 ccgccgtgac cgctcgaagc accaccgtgc ccattcaccg tgccgcccat tgcgttgctc
     2161 gctccggcga gcccgccgag cccggtcgcg gcgtcgccac cggcgctcgt cgagcctccg
     2221 gcaccgtcgt tcttctcctt gtcactgccg caccctgcga ccaacgcggc catggcccaa
     2281 ccgaacaatg tgaatcgatg tctgcgcatg gttctcagcg acccgctcac gcgctcgccc
     2341 caaaccaaca ttgtcgcctt ccaccgccgg gggcgcaacg gtttcgacgc ttcgcgggcg
     2401 cacacatcga cgcgcgaagt agcgaaccgt gcgcgcccgt gcgagtcgcg aacgctcccc
     2461 ttctgctacg gaagtcagtc gatgccgagc tcacccacgc gcgagacgat gcgtccgacg
     2521 agcccgtagt cccgcgcggc ctcggcgttc atccagtgat tgcgctgcgt ctcccgcgcg
     2581 atcttctcga tgggctggcc cgtcgcctcg gcgaagatgc gattcaaccg cgcgcgcatc
     2641 gaaagcacct gagaagcttc gatttcgacg tcagacgccg gaccggacgc tcccccgcgg
     2701 ggctcgtgta acagaaagcg ggtattcggt agcgacaagc gacgttcacg cggagccgcg
     2761 acatagacca gcgccgccgc gctcgcgacc caaccggttc cgactgcgac gactcgggcg
     2821 tcgacgaagc gaatcacgtc atggatggtg tcgccggcct cgacgtgtcc gcccggcgaa
     2881 ttgatgagaa ggcgaatcgg agcgtcgctc gcgctggcga gcgccagaat ctgcgcactg
     2941 actacttccg ccagctgcat cgtaatttcg ccgaacaaca gcaaagtcct ggctttccag
     3001 agctcgtcga acacgcgggg cggcggatga accgagggcg ggtttggggc atcggtgggc
     3061 atgactcgtg tttgatgtcg ctgcgccgga tctcccatga ccgaaacgct cgaaaacatg
     3121 acccgatgtg cgcgagccca atttcgcgtg gactcccgag cgcgggatcg gtgaaacttc
     3181 cgtcgatgaa gaagtcggac tcgcgcgttc gaacggtgca ccttggcgag gctcggatcc
     3241 tcctgtcctc gcccgagagc attctgctcg tcatcggcac ggggtgcatt ctgcgtaccg
     3301 ccgccggtac tcaaacgggc cttccgagcg ggacgttgct cgtgcgcgag caccaggagc
     3361 ggcggccgtt cgacggcgcc gtcgcgacgg caatcgaact gatgaagcag aagctcgagc
     3421 agccactgcg agtcagcgcg ttggcaaaag cggttggcgt ctcccgagcg gcgttcgcgc
     3481 ggcgcttcgt gcgcgcaacc ggcttttcgc cgttacgttt tctgcacacg ctgcgcctcg
     3541 agcgcgcggc gcagctcctc gtggagacgg accttacgtt gtccgacatc gcgagccgcg
     3601 tgggatacgt ttcggagttc gcgttcagcc gcgccttcaa gcgtcaccgc gggctgccgc
     3661 cgagcgtatt tcgcaggagc tcggccgcgc cacgcgcaat ggccctcgct gcctaatttg
     3721 tgtccgagct tcgacgaagt cgccgagcgt tacggcgaag ccgtggccgt tacggcgaag
     3781 gcgctgcccg acggcgctcg gcctgtgaca cgcggcggcc cgcctcgccc aaccatgccg
     3841 caaacagggc gaccgcgagg agagtcaaga ggacgccagc agggacccac atgaccgact
     3901 gcgacccgca acgaccccgc gcgcaaccgg cgcgcgaccg actgcgaccc gcgacgaccc
     3961 cgcgcgcaac ccgcaacgac cccgcgcgc
//
LOCUS       DASTKS010000165         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_25786,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000165 DASTKS010000000
VERSION     DASTKS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25786"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFQ35_03505"
     CDS             <1..264
                     /locus_tag="VFQ35_03505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9929722.1"
                     /translation="ILLPHTGKREAFVVGERLRNAIKNVEFHFGGDSLRISASIGISG
                     NIDVPTGNAEDLVKAADLALYEAKRSGRDRCMLFDDRNSLPPR"
     gene            complement(316..927)
                     /locus_tag="VFQ35_03510"
     CDS             complement(316..927)
                     /locus_tag="VFQ35_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929723.1"
                     /translation="MTRKLASLGFSIAAFVLGLSATREAHAVEYYRAGKVSVSLERVF
                     GLHYWHSSVEQANGLDSVSSGTVVGLGWYAAQSPYHLGRAAVDGFIIDRLSLGGSVGF
                     YSASGDNDDSGVLLYPRVGYVIPISRVFSFWPRGGFSFIKGDSSSLFGLAAEGMFVAS
                     PSDDWHILFGPTFDFGLTGHVNGDRDWSHFAIGFPAVGLMGTF"
     gene            995..1999
                     /locus_tag="VFQ35_03515"
     CDS             995..1999
                     /locus_tag="VFQ35_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9929724.1"
                     /translation="MRRQFLKGLLGFGALWLPVACKKGSDATPSANARLRIAVIPKGT
                     THEFWKSVHAGAVKAARELDVDIVWKGPLKEDDLKGQVDVVQSFTAQKVSGIVLAPLN
                     DKALVSSVNAAITAKVPVVIFDSAIAGGAITSFVATDNRAAGRLAGDRMAELLSGKGK
                     LILLRYQEGSASTHEREEGFLEAVKKHPGLELVSDNQYGGATTETAFAASERLLLSKN
                     AAAESALGVFTPNESTTFGMLLALQKAKLAGKAKFVGFDASDKLVQAVRAGEIDALVM
                     QNPFNMGYLSVKALVTALRGGQVEARTDTGAKLVDKANLDTPEIRELVKPDLEKWLGK
                     "
     gene            2007..>2858
                     /locus_tag="VFQ35_03520"
     CDS             2007..>2858
                     /locus_tag="VFQ35_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9929725.1"
                     /translation="MRLRVTGVDKSFGGTRVLEGVHLEVAPGSIHALLGENGAGKSTL
                     MKIVAGAIEPDRGTLELDGAPFVPASPAHAREQGVAIVYQELSVCPHLSVAENVCLGG
                     EPAKYGFVRRSELIERTKRALAPLTRGERRLDLDAPVSELSTGDRQLVEIARALAAEK
                     TRLLILDEPTSSLDGGSAERLFEALRELAASGIAILYISHFLEEVRRIADSFSVLRDG
                     QHVKSGRVADVSNAELVEAMAGRSVKSLERARRSPGEPLLSVRELAGRKLPVSASIEV
                     HRGEVLGI"
BASE COUNT          562 a          926 c          918 g          452 t
ORIGIN      
        1 attttgcttc cccacaccgg caaacgcgaa gcgttcgtgg tcggtgagcg gctcagaaac
       61 gcgataaaaa acgtcgaatt ccacttcggc ggcgactcgc tgcgaatcag cgccagcatc
      121 gggatttcgg gcaacatcga cgtgcccacc ggcaatgccg aagacctcgt caaggccgcc
      181 gacctcgcgc tctacgaagc caagcgctcc ggccgcgatc gctgcatgct gttcgacgac
      241 cgcaacagct tgccaccccg ctgaggcgcg cgtcgcaggg gacagcgcgc ctcacccgtc
      301 gggcgttcgc aatcgctaga aagttcccat caggccaacc gcggggaacc cgatcgcgaa
      361 atgcgaccag tcacggtcac cgttcacgtg cccggtgagc ccgaaatcga aggtgggtcc
      421 aaacaagatg tgccagtcat cgctgggcga ggccacgaac atgccttcgg cagccagccc
      481 gaacagcgaa ctgctgtcgc ccttgatgaa ggagaagcca ccgcgcggcc agaagctgaa
      541 gacgcgggag atgggaatca cgtagccgac gcgcggatac aaaaggaccc cgctgtcgtc
      601 gttgtcgccc gaggcgctgt aaaatccgac agagccgccg aggctcaggc ggtcgatgat
      661 gaagccgtcc accgcggcgc gcccgagatg atacgggctt tgcgccgcat accagccgag
      721 cccaaccacg gtcccgctac tcacgctgtc gagaccgttc gcttgctcga ccgagctgtg
      781 ccagtagtgc aagccgaata cgcgttctaa ggagaccgag actttgccgg cccggtagta
      841 ttcgaccgcg tgcgcttcgc gtgtggccga gagccccaag acgaatgcag cgatcgagaa
      901 gccgagagat gcaagctttc gcgtcatgcc gccagttatc ctccggctcg cgggcgctgt
      961 tcaaccagaa ctagaaccgt gttaggaaat tccggtgcga cgccagtttt tgaagggatt
     1021 gctcggcttc ggtgcgcttt ggctacccgt tgcgtgcaaa aagggcagcg acgccacgcc
     1081 ttcggccaac gcgcgactcc gcatcgcggt aattcccaag ggcaccacgc acgagttttg
     1141 gaagagcgtg cacgcaggcg cggtcaaggc ggcgcgcgag ctcgatgtcg acatcgtctg
     1201 gaaagggccc ttgaaggaag acgatctcaa ggggcaggtc gacgtcgttc agtccttcac
     1261 cgcgcaaaaa gtgtcgggca tcgtgctcgc tccgctgaac gacaaggccc tcgtgagctc
     1321 ggtgaacgcg gcgataacgg ccaaggtccc ggtggtcatt ttcgactcgg cgatcgcggg
     1381 aggagcgatc acgagcttcg tcgcgaccga taaccgcgcc gccggccgtc tcgcgggcga
     1441 tcgcatggcg gagctcctgt ccggcaaggg caagctcatc ttgcttcgct accaagaagg
     1501 ttcggcgagc acccacgagc gcgaagaggg cttcctcgag gcggtcaaaa agcacccggg
     1561 cctcgagctc gtgagcgaca accagtacgg cggcgccacc accgagaccg ccttcgccgc
     1621 atcggagcgc ttgttgttgt cgaagaacgc ggcagccgag agtgcgctcg gtgtcttcac
     1681 cccgaacgaa tcgactacct tcggcatgtt gctcgctttg caaaaagcga agctcgcggg
     1741 caaagccaag ttcgtcggct tcgacgcttc ggacaagttg gtgcaggcgg tgcgcgcggg
     1801 cgaaatcgac gcgctcgtca tgcaaaaccc gttcaacatg ggctacctct cggtgaaggc
     1861 gctcgtcacg gcgctgcgcg gcggtcaggt cgaagcgcgc accgacacgg gcgcaaagct
     1921 cgtcgacaaa gcgaacctcg acacgcccga aatccgcgag ctcgtgaagc ccgacctcga
     1981 gaagtggctc ggcaaatagg cccctgatgc gcctccgcgt gacgggtgtc gacaagtctt
     2041 tcggcggcac gcgcgtgctc gaaggcgtcc atctcgaagt cgcgcccggc tccattcacg
     2101 cgctgctcgg tgaaaacggc gccggcaaga gcacgctgat gaagatcgtg gccggtgcca
     2161 tcgagccaga tcgcggcacg ctcgagctcg acggcgcgcc cttcgtcccc gcgagcccag
     2221 cgcacgctcg cgagcaaggc gtcgccatcg tctaccaaga gctctccgtg tgcccgcacc
     2281 tgagcgtcgc ggagaatgtt tgtttggggg gtgagcccgc gaagtacggc ttcgtgcggc
     2341 gttccgagct catcgagcgc acgaagcgag cgctcgcgcc gctgacgcgc ggtgagcgcc
     2401 gactcgacct cgacgcgccc gtcagcgagc tctcgaccgg agaccgtcag ctcgtagaaa
     2461 ttgcgcgcgc cctcgccgcc gaaaagacgc gcttgctgat cttggacgag ccgacctcga
     2521 gcctcgacgg gggttcggcg gagcggctct tcgaagcctt acgcgagctc gcggcgagcg
     2581 gcatcgccat cctctacatt tcgcacttcc tcgaagaggt gcggcgcatc gcagatagct
     2641 tcagcgtgct gcgcgatggc cagcacgtaa agagcggccg cgttgccgat gtgagcaacg
     2701 ccgagctcgt agaggccatg gctggccgca gcgtaaaaag cctggagcga gcgcggcgca
     2761 gccccggcga gccattgctc agcgtgcgag agctcgcggg aagaaagctg cccgtctcgg
     2821 cgagcatcga ggtccaccgc ggagaggtgc tcggcatc
//
LOCUS       DASTKS010000166         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1140417, whole genome shotgun sequence.
ACCESSION   DASTKS010000166 DASTKS010000000
VERSION     DASTKS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140417"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VFQ35_03525"
     CDS             <1..1495
                     /locus_tag="VFQ35_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929726.1"
                     /translation="VFYDAEEQQLMSVAVAPDGTVYAGASDKAKLYRVTGPGRASVLY
                     DFGRTEVRAIAVAKNGDVYAIANEIKPGSQLPSRQPRTSDSAPAGPITPASPAKGKGT
                     LYRFSPDGRPDQLLDDGDEHYTSLVLGEDGRPYVGTGVEGRVYSVDQNHNSVLVADIE
                     ERQVTAIVFNGKQGLVIGSDPAVVHPITGVGGADAIWTSKVLDAGIRARFGRMDWEAT
                     GALQFSTRSGNTREPDETWSAWSKEVDVPSKVESPPARYFQVRARFAKDPKAVLTDVD
                     VSFVTDNLRAVVTEITAKPGGDRGNNEEGLKSSGGPVQRKPETTLNITWKVDNPDKDE
                     LRYRLKYRLVGSNTWYDLTKPTEKVSKENYTWDTSDLPEGRYRLKVVATDELANPADR
                     VTTDQMESGVVLVDNTPPIVEGLKLVGRRLQATVIDGVGPVSRVEISVAGSDEWRPLA
                     ASDGIFDEQRESVDADLSQVLPAGPALVSVRAYDGADNFVVRSLPLR"
     gene            1509..2417
                     /locus_tag="VFQ35_03530"
     CDS             1509..2417
                     /locus_tag="VFQ35_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9929727.1"
                     /translation="MPSRPTDHLGRGAASMVGAALVFAVMSACVKVAARELPNTMVVF
                     ARNLAGLLWLAPWVLGSKRPSLRTERMGEHLLRAVAGIASMYCFFFAISRLGLAEAML
                     LNYSLPLFVPLVERVWLGEPIPAKIWPALGLGSVGLALILKPGASMFQPAALIGALSA
                     LLSAIAQVGVRGLTRSEPVRRIVFYFTLFSTAIALFPASYQWVTPRIQLLPLLGLLGF
                     CAIGGQLLLTRAYSLAPAVRVGPFMYSAVVFAALFDWLLFRKVPDALTWGGAACVVVA
                     GVLALRLRMAAPASAPPPLDAIGSER"
     gene            2427..>2776
                     /locus_tag="VFQ35_03535"
     CDS             2427..>2776
                     /locus_tag="VFQ35_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929728.1"
                     /translation="MGVVERVIPAAALGYGIFAAWFSLAHGVPGILVTVIAVLPLGLI
                     ALALERVFPERTDYSRADQPVRVELAHYLFDYNVGYALALGACALLDWGLARYSTFHP
                     WPNEWPLVVQIAVAA"
BASE COUNT          429 a          918 c          927 g          502 t
ORIGIN      
        1 ggtgttttac gacgccgaag agcagcagct gatgagcgtc gcggtcgcgc cggacggcac
       61 cgtgtatgcg ggcgcgagcg acaaagccaa gctttatcga gtgaccggac cggggcgtgc
      121 gagcgtcctc tacgacttcg gtcgcacgga ggtacgcgcc atcgccgtcg ccaagaacgg
      181 cgacgtgtac gccatcgcga acgaaatcaa gcccggctcg cagctgccgt cacgacagcc
      241 gcgcacctcc gactcggcgc cggctggccc catcactccc gcgagccccg cgaaaggcaa
      301 gggcacgctc taccgctttt cgcccgacgg caggcccgat cagctgctcg atgatggcga
      361 cgagcactac acgagcctcg tgctcggtga agacgggcgt ccttacgtcg gcacgggcgt
      421 ggaggggcgc gtctacagcg tcgatcagaa ccacaactcg gtcctcgtcg ccgacatcga
      481 agagcgtcag gtcaccgcga tcgtcttcaa cggcaagcaa ggactagtca tcgggagcga
      541 ccccgccgtc gtgcacccga tcacgggcgt gggcggcgcg gacgcgattt ggaccagcaa
      601 ggtcctcgac gccgggattc gcgcgcgctt cggtcgcatg gattgggagg ccaccggcgc
      661 gcttcagttc tcgacccgca gcggtaacac tcgcgagccc gacgagacgt ggagcgcttg
      721 gagcaaagaa gtcgacgtcc cctccaaagt cgagagcccg ccggcgcgct acttccaggt
      781 gcgcgcgcgc ttcgcgaaag atccgaaggc ggttctcacc gacgtcgacg tctccttcgt
      841 caccgacaac ctgcgcgccg tggttaccga aatcaccgcc aagccgggcg gcgatcgcgg
      901 caacaacgaa gagggcctga aatcctcggg cggccccgtg cagcgcaagc ccgagaccac
      961 gctcaacatc acctggaaag tcgacaaccc cgacaaggac gagctccgct atcggctcaa
     1021 gtatcgcctc gtcggctcga acacctggta cgatctcacg aagcccaccg agaaggtctc
     1081 gaaggagaac tacacgtggg acacctcgga tttgcccgag gggcgttacc ggctcaaggt
     1141 cgtcgctacc gacgagctcg cgaacccggc ggatcgagtc accaccgatc agatggagag
     1201 cggcgtcgtg ttggtcgaca acacgcctcc catcgtggag ggcttgaagc tcgtggggcg
     1261 tcgcttgcaa gcgaccgtca tcgacggtgt gggtccggtg tcgcgcgtcg aaatcagcgt
     1321 cgccggcagc gacgagtggc ggccgctcgc ggcgagcgac ggcatcttcg acgaacagcg
     1381 cgagtccgtc gacgcggacc tttcgcaggt tttgcccgcg ggtccggcgc tcgtgagcgt
     1441 acgcgcctac gacggagccg ataacttcgt cgtgcgcagc ttgccgttgc gctgagcgct
     1501 ggcccgcggt gccaagtaga ccgacggatc acctcgggcg cggagcggcc agcatggtcg
     1561 gcgcagcgct ggtgttcgcg gtgatgagcg cttgcgttaa ggtcgcggcg cgcgagctac
     1621 cgaacacgat ggtcgtgttc gcccgcaatc tcgccgggct gttgtggctc gcgccgtggg
     1681 ttttggggag taagcggccg agcttacgta ccgagcgcat gggcgagcac ctcttgcggg
     1741 cggtcgcggg catcgcctcg atgtactgct tcttcttcgc gatttcacgg ctcgggctcg
     1801 cggaggcgat gctgctcaac tactcgctcc cgcttttcgt tccgctggtg gagcgtgttt
     1861 ggctcggcga gcccatcccc gcgaagattt ggcctgccct cggtctcggc tcggtcggtc
     1921 tcgcgctcat cttgaagccc ggtgcctcga tgtttcagcc ggcggcgctg atcggcgccc
     1981 tctccgcgct gctgtcggcg atcgctcaag ttggggtgag ggggctgacg cgctcggaac
     2041 cggttcggcg catcgtcttc tatttcacgc tcttctcgac ggccatcgcg ctctttccgg
     2101 cgagctacca atgggtaacg ccccggatcc agttgctacc gctgctcggg ctgctcgggt
     2161 tttgcgcgat cggcgggcaa ctcttactga cgcgcgccta ctcgctcgct cccgcggtac
     2221 gtgtgggccc gttcatgtac agcgccgtcg tgttcgcggc gctgttcgac tggctacttt
     2281 tccgtaaggt tcccgatgcg ttgacttggg gtggtgccgc gtgcgtcgtg gtagcgggcg
     2341 tgctcgcgct gcgattgcgc atggcggcgc cggcgtcggc gccaccgccg ctggacgcca
     2401 tcggctccga aaggtaagca ccacgaatgg gcgtggtcga gcgggtgatc cccgcggcgg
     2461 ctctcgggta cggcatcttt gccgcttggt tttcgctcgc gcacggcgtc cccgggattc
     2521 tggtcaccgt gatcgcggtg ctgccgctcg ggctcatcgc gctcgcgctc gagagggttt
     2581 tcccggaacg caccgactac agccgggccg accagcccgt tcgggtcgag ctcgcccact
     2641 atttgttcga ctacaacgtg ggttacgcac tggcgctcgg cgcttgcgcg ctgctcgact
     2701 gggggctcgc gcggtattcg acgtttcacc cgtggcccaa cgaatggccg ctcgtggtgc
     2761 aaatcgccgt ggccgc
//
LOCUS       DASTKS010000167         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_26026,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000167 DASTKS010000000
VERSION     DASTKS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26026"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VFQ35_03540"
     CDS             <1..1208
                     /locus_tag="VFQ35_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HET9929729.1"
                     /translation="AALAVQGEAADARLVAYVVERRPLGDDALREFLRQVLPEYMLPA
                     HFRALSSLPTNANGKLDRKALPKVDSRPSAGVLPRDELERTLASAFAGILRTESVGIF
                     DNFFELGGHSLSAAELAFRLSDELGVELPVKALFESPTVAGLAATLRRGDSSEFGRGI
                     VVARRGSGTPWFCFPALAGTSAPYLAAITPVGAPVFLLEAPGVDSGSPLTSVESLAQH
                     FRTLVESAAPSGPVRLLGWSFGAVTAFETAAALASHGREVAELVLVDPALPGARAPRD
                     LAETFVMDAAESLDEVGALEPLSALSRERIEPADLFARARDIGVFSKRVGLQEIERRF
                     SVYVASWRALEEYRCAQRYAGRTVILAATQGNGRSAERWLEVLPRAELSSIEANHYTI
                     LGQLRGLLR"
     gene            1251..>2161
                     /locus_tag="VFQ35_03545"
     CDS             1251..>2161
                     /locus_tag="VFQ35_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929730.1"
                     /translation="MNLKRLSGVALLLGSAGLISVEPSCSLVVNDFKEQCQTTQDCID
                     LANRQPKLPNGQPSVTGLVCSDKHVCEPEKGCHSNAECQAAHNGEPYICRASDRTCQS
                     LILKSTTEPDKTICDVLADPADFKNENTIWLGASVFFKEASYQGLELVRQDFNKFAMG
                     LPPATATSTERRPLGFVYCEADGPLEEGAQHLVNDLELPVVITSLDTTSEITLLTKYS
                     IANGKKVFQLSTSAGGPLLKTVDNKGQLIDLVLINENYDKETVELVQDYYVPLLRKSG
                     GPLTAQQSPRVAVIHSSTPTYANTASK"
BASE COUNT          390 a          702 c          661 g          408 t
ORIGIN      
        1 acgcggctct ggcggtgcag ggcgaagccg ccgacgcgcg cctcgtcgct tacgtggtgg
       61 aacgtcgtcc gctcggcgac gatgcactgc gcgagttcct gcgccaggtg ctccccgaat
      121 acatgctccc ggcgcatttt cgcgcgctct cgagcttgcc gaccaacgcc aacggcaaac
      181 tcgatcgcaa agcgctaccc aaggtggact cgcgaccctc ggcgggcgtt ttgccgcgcg
      241 atgagctcga acggacgcta gcttcggcct tcgccggcat cctgcgaacc gaaagtgtcg
      301 gcattttcga caactttttc gagctcggag gacactcact gtccgccgcg gagctcgcgt
      361 ttcgcttgag cgacgagctc ggtgtcgagc tccccgtgaa ggcgcttttc gagtcgccca
      421 cggtcgcggg gttggcagca actctcaggc gaggggactc gagcgagttc ggccgcggca
      481 tcgtggtcgc gcgccgtggt tcgggcacgc catggttctg ttttcctgcg ctcgccggaa
      541 cctctgcgcc gtacctcgca gcgatcactc ccgtcggggc tccggtgttt ctgctcgagg
      601 ctcctggcgt cgacagcggg agcccgctca ccagcgtcga gagtcttgct cagcactttc
      661 gcactctcgt ggaaagcgca gccccgagcg ggccggttcg gctgctgggt tggtcgtttg
      721 gcgctgtaac ggccttcgaa acggcggcgg cattggcttc acacggtcgc gaagtagccg
      781 agctcgtcct cgtcgaccct gcgttgcccg gtgcgcgcgc gccgcgcgac ctcgccgaaa
      841 cattcgtgat ggatgccgcc gagtcgttgg acgaggtagg tgctttggag cctctcagcg
      901 cgctctcgcg agaacggatc gagcccgctg acctgttcgc tcgcgcgcgc gacataggag
      961 ttttttcaaa gcgcgttggg ctgcaagaaa ttgaacgacg cttttcggtg tacgttgcaa
     1021 gctggcgcgc gctggaggaa tatcggtgcg ctcaacgcta cgccggccga acggtgattt
     1081 tggccgcgac gcagggtaac ggtcgaagtg ccgagcgctg gctcgaggtt ctgcctcgcg
     1141 ctgagctctc gagtatcgag gcgaaccact acacgattct tggccaactg cgcgggcttc
     1201 tccgctagag ttccgcgcgg ggcatcaagc gcacacttcg aggaattgga atgaatctta
     1261 agcgactttc cggcgtggct ctcctgctcg gttccgcggg gctcatcagc gtagagccgt
     1321 cgtgctcact ggtagtgaac gacttcaaag agcagtgcca gaccacgcag gactgcatcg
     1381 atctcgccaa ccgccaaccc aagcttccca acggccagcc cagcgtcacc ggcttggttt
     1441 gctcggacaa acacgtgtgc gagcccgaaa agggctgcca ctcgaacgcg gaatgccaag
     1501 ccgcgcacaa cggcgagcca tacatctgcc gcgcttcgga ccggacttgt cagtcgctga
     1561 ttctcaagtc gaccactgag ccagacaaaa ccatttgcga cgtcttggcc gacccagccg
     1621 acttcaagaa cgaaaacacg atctggctcg gcgcgagcgt tttcttcaag gaggcgtcgt
     1681 accaaggcct cgagctcgtc cggcaagatt tcaacaagtt cgcgatgggt ctcccgccgg
     1741 cgacggcgac gtcgaccgag cggcggcccc tcgggttcgt ctactgcgaa gccgacggcc
     1801 ccctcgagga gggagcgcaa cacctcgtca acgatctcga gctgccagtc gtgatcactt
     1861 cgctcgacac gacctcggag atcacgctcc ttaccaagta ctcgattgcg aacggcaaga
     1921 aggtcttcca gttgtctacg tccgcgggcg ggcccctgct caagacggtc gacaacaaag
     1981 ggcagctcat cgatctggtc ctcatcaacg agaactacga caaggagacg gtcgagctcg
     2041 ttcaagacta ttacgttcct ctactccgca agtcgggtgg gccgctgacc gcgcagcaat
     2101 caccgcgcgt tgccgtcatc cactcgagca cgccgacgta cgcgaatacc gccagcaaaa
     2161 t
//
LOCUS       DASTKS010000168         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_917182, whole genome shotgun sequence.
ACCESSION   DASTKS010000168 DASTKS010000000
VERSION     DASTKS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917182"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFQ35_03550"
     CDS             <1..902
                     /locus_tag="VFQ35_03550"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HET9929731.1"
                     /translation="KGSMQPALEELLAELVLGAELPPTEPRRLVAWLEQRGVSSPDAQ
                     AIAETELTGLLTYRELVRQTLRGAVERAIPRSMARLGDTFDVFFERFLAEQGPRTNYL
                     RDVTRELLDFIDGSDRAGLPGYWLDLARHESLHIEVAAAPAATRGRPGAALDLEAGLG
                     FSESVRLCQYDYAVHELPDDLADRTLPVREPTHILAYRDAEHDVRYLALSAFAATLLR
                     GLLAGKSLGESLLGASREHGVPLDEANLNATAALLANLSDRGVLMGPCAIGSARADDP
                     ANLGTGRAAVPHPSPEPREAKRS"
     gene            899..>2703
                     /locus_tag="VFQ35_03555"
     CDS             899..>2703
                     /locus_tag="VFQ35_03555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HET9929732.1"
                     /translation="MTELDPLHIVGQIVADKYRIEKLVGEGGFAVVYRAQHTIWNKPV
                     AIKFFNGLSSAPMSQRDLFQQAFVQEGALLTELSSQTAGIVQARDIGTYTTPDGSWMP
                     FMVLEWLEGSSLEGVLEREQRAKAPPWTVEQVVRLLGQAARALDVAHTRGIAHRDVKP
                     ANLFVLGNDARSPDATLKVLDFGVAKMVTDNTQLQAALAKTGTSVTSFTPQYGAPEQF
                     SRNLGATGPWTDVFALALVATELLTGRPALDGDDVVQLAVSASQPNNRPTPRSLGATV
                     SNQVEAVFQKALAVKPTDRYSRAAEFWNALEAASGNLTRTQTQPNSAVIAPTELAENP
                     LAAEAALRVTGANPTTGAHSTIATEARPSTRSNAPWLIAAAAAIVAVGAGVALSKNRD
                     SASTQAPSAQVAAVSAAPTPPAPPPEPTCPERMVKIPRGQFFQGSDAKDARDNEKPAH
                     QVSLDAFCVDLYEVTAGDYKACSDSGKCKRASTEVEWPGITPADKKVYGPLCTIADPA
                     RASHPINCVTWEMANLYCKAQDKRLPTESEWEYATRGPDGRVYPWGDDAPTAKHLNAC
                     GSECVAWGKDHKVALEALYPESDGFPTTAPVGQYEA"
BASE COUNT          494 a          953 c          860 g          396 t
ORIGIN      
        1 cgaaaggaag catgcagccg gcgcttgaag agctgctcgc cgagctcgtg ctcggcgccg
       61 agctgccgcc gaccgaaccg cggcggctcg tggcttggct cgagcagcgc ggcgtcagct
      121 ctcccgatgc gcaggcgatc gccgagacgg aactgacggg gctccttacg tatcgcgagc
      181 tcgtgcgcca gacgctgcgc ggcgcggtcg aacgcgccat cccacgcagc atggctcggc
      241 tgggggacac cttcgatgtc ttcttcgagc gcttcttggc ggagcagggg ccgcgtacga
      301 actacttgcg cgacgtgacg cgtgagctcc tcgacttcat cgacgggagc gaccgcgccg
      361 gtctccccgg gtactggctc gatctcgccc ggcacgagtc gctgcacatc gaagtggcag
      421 ccgcccctgc cgcaactcgc ggccgccccg gagccgcgct ggatttggag gcggggctcg
      481 gcttctccga gtccgtccgg ctttgccagt acgactacgc agtgcacgag ctgcccgacg
      541 acctcgccga ccgcacgtta ccggtgcgcg agcccaccca catcctcgct tatcgggacg
      601 cggagcacga tgtgcgctac ctcgctctca gcgcgttcgc ggccacgctg ctcagggggc
      661 tgctagcggg aaaatcgctg ggagagagcc tgctcggcgc ctcgcgcgag cacggtgttc
      721 cgctcgacga ggcaaacttg aatgccacgg cagcgctgct cgccaatctg agcgaccggg
      781 gggtgctcat gggaccctgc gcaatcggtt ccgctcgcgc agacgatccg gctaacctcg
      841 ggaccgggag agcagccgtt ccccacccct cacccgaacc tcgagaagca aagcgttcat
      901 gaccgagctc gacccgctcc acatcgtcgg ccagatcgtc gcagacaagt accgcatcga
      961 aaagctcgtc ggcgagggcg gcttcgccgt cgtctatcga gcccagcaca cgatttggaa
     1021 caagcccgtc gcaatcaagt tcttcaacgg cttgtcgtcg gcgcccatga gtcagcgcga
     1081 cctgtttcag caggcgttcg tgcaagaggg cgcactgctc acggagctgt cgagccagac
     1141 cgcaggcatc gtgcaagcgc gcgacatcgg gacgtacacg accccggacg gaagctggat
     1201 gccgttcatg gtgctcgagt ggctcgaggg cagctcgctc gaaggcgtgc tcgagcgcga
     1261 gcagcgggca aaggccccgc cttggaccgt cgaacaagtc gtccgcctgc tggggcaagc
     1321 ggcgcgcgcc ctcgacgtcg cgcacacgcg gggcatcgcg caccgcgacg tgaagcccgc
     1381 gaacctcttc gtgctcggca acgacgctcg ctctccggac gctacgttga aggtgctcga
     1441 cttcggcgtc gccaaaatgg tgaccgacaa cacccagctc caagccgcgc tcgccaagac
     1501 aggcaccagc gtcacgtcgt tcacgccgca gtacggagcc cccgaacagt tcagccgaaa
     1561 cctcggcgcg acgggcccgt ggacggacgt cttcgcgctc gcgctcgtcg caacggagct
     1621 cttgacgggg cgccccgcgc tcgacggtga tgacgtcgta cagctcgcgg tgagcgcgag
     1681 tcagcccaac aatcgtccca cgccgcgctc cctcggcgcg acggtttcga accaggtcga
     1741 agccgtattt cagaaggcgc tcgccgtaaa gcctacggat cgctactcgc gcgcggccga
     1801 attctggaat gcgctcgaag cggcgagcgg gaacctcacc cgcacgcaga cgcagccgaa
     1861 ctcggccgtc atcgccccga ccgaactcgc ggagaacccg ctcgcggctg aagccgcgct
     1921 ccgcgtcact ggcgccaacc cgaccacggg tgcgcactcg accatcgcca ccgaagcgcg
     1981 tccgtcgacc cgcagtaacg cgccctggct cattgcagcc gccgccgcga tcgtagcggt
     2041 aggcgctgga gtggcgctga gcaaaaatcg agactcggcc tcgacgcagg ctccgagcgc
     2101 tcaggtcgcg gccgtgtcgg cagcgccgac gccacctgct ccgcctcccg agcccacctg
     2161 ccccgagcgc atggtgaaga ttccgcgcgg gcagttcttc caaggctcgg acgcgaagga
     2221 cgcgcgcgac aacgagaaac cagctcacca agtctcgctc gacgctttct gcgtggactt
     2281 gtacgaggtg acggccggcg actacaaagc ctgttcggac agcggcaaat gcaaacgtgc
     2341 ttcgaccgaa gtcgagtggc cgggcatcac tcccgcagac aaaaaggttt acggcccact
     2401 ctgcacgatc gccgacccgg cgcgcgcgag ccatcccatc aactgcgtta cctgggagat
     2461 ggcgaacctc tactgcaaag cgcaagacaa gcgccttccc accgaatcgg agtgggagta
     2521 cgcaacgcgc ggccccgatg gccgagtcta cccgtgggga gacgacgctc cgacggcgaa
     2581 acacctgaac gcttgtggtt cggagtgcgt ggcctggggc aaagaccaca aagtcgcact
     2641 cgaggcactc tatccagaga gcgacggctt cccgaccaca gcgccggtcg gccaatacga
     2701 ggc
//
LOCUS       DASTKS010000169         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1140859, whole genome shotgun sequence.
ACCESSION   DASTKS010000169 DASTKS010000000
VERSION     DASTKS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140859"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFQ35_03560"
     CDS             complement(<1..811)
                     /locus_tag="VFQ35_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929733.1"
                     /translation="MRNPLNLRNLPSRSYVPSYVENVFAEENPFRAKPRRNVERWPSF
                     EEVRAALPEPHYPARPEAVECYYRCWQIAFSNFRRATEVNGFVSDFSSTMFEDSLFLW
                     DSVFITLFGRYGDRAWKFQNTLDNFYAKQHPDGGICRQFLEEDGSECFSRFDPAGTGP
                     NVLGFSEWEYFLFTRDEARLAEVFPALVAYGQWCRRYRTWPNGAYWGTGWSSGMENQP
                     RTPAGAHKLFDHAHRTWVDTNMQQVLANRIVLDMAEVLDRKNDVADFAEENS"
     gene            complement(995..1807)
                     /locus_tag="VFQ35_03565"
     CDS             complement(995..1807)
                     /locus_tag="VFQ35_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9929734.1"
                     /translation="MKPDRRSSLGPVGLVIYNLFYWPYLLATVILAFFPALALYAGTF
                     WQRPRRWLNTYTAVWGAHYLSWAPFAGVRVEGRERAFTNGPAVFVVNHQSMVDILAVY
                     ALRAPFLWVSKVENFYVPFLGWNMWLNGYVPLRRGHLPSIRRMLRDCESRLRQGASLC
                     VFPEGTRSRDGNLRSFYRGAFWIAARYRVPVIPIVVDGTFSILKKGHFGIRPRPVTLR
                     VLEPLLPEDFGHDDRRLRDAVYERMERELASMRSPEKAEATALARIELRSES"
     gene            1900..2484
                     /locus_tag="VFQ35_03570"
                     /pseudo
     CDS             1900..2484
                     /locus_tag="VFQ35_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            2587..2862
                     /locus_tag="VFQ35_03575"
     CDS             2587..2862
                     /locus_tag="VFQ35_03575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9929735.1"
                     /translation="MHPKPTDTVSVHYSGWTTDGQLFDSSVQRGAPTSFALNQVIKGW
                     TEGVQLMVEGEKTRFWIPGNLAYGDKPARPGAPAGMLVFDIELLQIR"
     gene            complement(2902..3591)
                     /locus_tag="VFQ35_03580"
     CDS             complement(2902..3591)
                     /locus_tag="VFQ35_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9929736.1"
                     /translation="MAPVAGLELERASFRYGELWVLRETSLVVERGQNLVVTGDNGVG
                     KSTFLYVCAGLLPATTGRVLLGGLPANPRRPSDLFRSGVRRGVVFYSGGLLSNSSALN
                     NVILPLRYHADVLGIDEREIDQRARELLSDLRVSQSDFHTLPAHLSVGVRRRVSLARA
                     LAIRPNFLFFDDPDANLDGTTRRIVYELLERFRDDVGVTLVLTTTSRELIDRLGIQVR
                     ELAHGHLLERV"
     gene            complement(3593..>3870)
                     /locus_tag="VFQ35_03585"
     CDS             complement(3593..>3870)
                     /locus_tag="VFQ35_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929737.1"
                     /translation="QSVLGPSPVELRSGIARALLPHDLVLFVSKGVGLGTILGWFACH
                     YGLKVKSSPTEVPRQASAAVVKTLLGCVAYDTALTVLFYWLAGPPLR"
BASE COUNT          775 a         1237 c         1259 g          599 t
ORIGIN      
        1 ccgagttttc ttcagcgaaa tcggcgacgt cgttctttcg atccagcacc tcggccatat
       61 cgagcacgat gcgattcgcg agcacctgct gcatgttggt gtcgacccaa gtgcggtgcg
      121 cgtgatcgaa cagcttgtga gcgcccgccg gcgtacgcgg ctggttctcc ataccgctcg
      181 accagccggt gccccagtaa gcgccgttcg gccaggtgcg atagcgacgg caccattggc
      241 cgtacgcgac gagtgccggg aacacctccg cgaggcgcgc ctcgtcacgc gtgaagagga
      301 agtactccca ctcgctgaag ccgagcacgt tgggcccggt gccggcgggg tcgaagcgcg
      361 agaagcactc gctaccgtct tcttccaaga actgtcggca aatccccccg tcggggtgct
      421 gctttgcgta gaagttgtcg agcgtgttct ggaacttcca cgcccgatca ccgtaacggc
      481 cgaacagcgt gatgaagacc gagtcccaca ggaacagcga gtcttcgaac atcgtcgacg
      541 aaaagtcgga gacgaagccg ttcacctccg tcgcgcgacg gaaattcgag aacgcgatct
      601 gccagcaacg gtagtagcac tcgaccgctt cgggacgggc tgggtagtgc ggctccggta
      661 acgcggcccg cacctcttcg aatgacggcc agcgctcgac gttccggcgc ggcttggcgc
      721 ggaacggatt ctcttctgcg aaaacgttct ccacatagct cggcacgtaa gaccggctgg
      781 gcaggtttcg caggttgagc gggttccgca tgctggtagc gcagggtact cattatcacg
      841 cgaccgttcg aaccaaaagt cgccgtgaat tcgactgatc aatccgacat ggtcacattt
      901 cgccgtggct gctcggagaa agcgcggtcg agcaagtcct ccgcagtgac gccggaacga
      961 ggcagactcg ccagtgcccg ccgcgccatc gaactcagga ttcggagcga agctcgatgc
     1021 gggcgagcgc ggtagcctct gccttctcgg ggctcctcat gctcgcgagc tcgcgctcca
     1081 tgcgctcgta caccgcgtct cgcagcctgc gatcgtcgtg accgaagtcc tcgggcaaga
     1141 gcggctcgag cacgcgcagc gtgaccgggc gcggccgaat tccgaaatgc ccctttttga
     1201 gaatggagaa ggttccgtcc acgacgatag ggatcacggg aactcgatag cgagccgcga
     1261 tccaaaatgc gccgcggtag aacgatctca ggttgccgtc ccgcgaccgc gtaccttccg
     1321 gaaacacaca cagactcgcc ccttgccgca gccgtgactc acagtcgcgc agcatccgcc
     1381 gaatgctggg caagtgaccg cgacggaggg gcacgtagcc attgagccac atgttccagc
     1441 cgaggaacgg aacgtaaaag ttctcgactt tactcaccca gaggaatgga gcgcgcagcg
     1501 catacactgc gaggatgtcc accatgctct ggtggttcac cacgaacacg gccgggccgt
     1561 tggtaaacgc tcgctcacgc ccctcgacgc gcacgcccgc gaagggtgcc cacgaaaggt
     1621 agtgagctcc ccacaccgcc gtgtacgtgt tcaaccagcg acggggacgc tgccagaaag
     1681 tccctgcgta cagcgcgagc gcgggaaaga acgccaaaat gaccgtcgcc aagaggtacg
     1741 gccaatagaa caggttgtag atgacgaggc cgacggggcc gagcgaagaa cgccggtcag
     1801 gcttcacgcc gcaaagctag cgcgtttttc tggcgccacc gagcgtccga gcgaagcggg
     1861 gttgaaaact cgacgagttt ccgggaagct tcgcccgcca tgaagtctgc tcgttgcctg
     1921 gtactttcgg cgttggttct ctcggttggc ctcggctgca acaagaagga gccggaaccc
     1981 accgcctcgg cgcccgagca accccctgcc gcggcgcctc cgccagcagc atctgtcccg
     2041 gcgcccgctg ccgaagccac accttcggcc gctgccgaag tcgcggccgc accgagcgcc
     2101 gccgcagccc cggctggaat ccccgccccc gatgacgtcg ccgctccgcc caaggacgca
     2161 aagaagacgg cctcggggtt ggccagcaag gtcctcaagc cgggcacggg caaagagcgc
     2221 ccgggcgacg tcgaccgggt gaaggtgcac tacacgggct ggaccaaaga cgggaagatg
     2281 ttcgactcgt cggtgcagcg cggtgagccc acgacgttcg gcgtgacgca agtaatccca
     2341 ggttggaccg aagcgttgaa gctgatggcg gtgggagaga agcgtcgcct ttggatcccc
     2401 gccaagctcg cctacggcga gaaagctccc cccggcgcgc ccgccggcga cctgaccttc
     2461 gacgtggagc tactcgaaat cgtcgcagcg ccgaagacgc ccgaagacgt gaaggcagcg
     2521 ccaaagacgg caaagaagac ggagtcgggg ctcgcctacc gcattttgaa gcccggtacg
     2581 ggcaaggtgc atccgaagcc gaccgatacg gtcagcgtgc actacagcgg ttggaccacc
     2641 gacgggcagc tcttcgattc gtccgtgcag cgcggcgccc cgacgagctt cgccctgaat
     2701 caggtgatca agggttggac cgaaggcgtg cagctgatgg tggaaggcga gaagacgcgg
     2761 ttctggatcc ccggcaatct cgcttacggc gacaagccgg cgcgcccggg cgcccccgcg
     2821 ggcatgctgg tgttcgacat cgagctcctg cagatccgct gagcctcgag gccagttcga
     2881 cctcactcga gagcgcgtcc ttcagaccct ctcgagcagg tggccgtggg cgagctcgcg
     2941 tacctgaatc ccgagcctat cgatgagctc gcggctcgtc gtggtgagca cgagggtgac
     3001 gccaacgtcg tcgcgaaagc gctcgaggag ctcgtaaacg atgcgccgcg tcgtgccgtc
     3061 gaggttcgcg tccggatcat cgaagaacaa gaagttcggg cgaatcgcga gtgctcgagc
     3121 gaggctcacg cggcgacgca cgccaacgga gaggtgcgcc ggcaaggtgt gaaaatcgct
     3181 ttgcgacacc cgaaggtcgg aaaggagctc gcgcgcgcgt tggtcgatct ctcgttcgtc
     3241 gatgccgagc acgtcggcgt gataacggag cgggaggatg acgttgttga gcgcggacga
     3301 gttcgagaga agtcctccgc tgtagaagac gaccccgcga cgcacgccgc tgcggaaaag
     3361 gtcgctcggg cgccgtgggt ttgcgggaag accgccgagc agcacgcgcc cggtagtcgc
     3421 cggtaagagc ccggcgcaca cgtagaggaa ggtcgattta ccgacgccgt tgtcacccgt
     3481 cacgactagg ttttgaccgc gctcgacgac gagcgacgtc tcacgcaaaa cccagagctc
     3541 tccgtagcga aaactcgcgc gttcgagctc gagaccggcg acgggagcca cgtcagcgca
     3601 gaggcggccc agcgagccag tagaagagca cggtgagcgc ggtgtcgtac gcgacgcagc
     3661 cgagcaacgt ctttacgacg gccgcgctgg cttgacgagg cacctccgtc ggcgagctct
     3721 tcactttcaa gccgtagtgg caagcgaacc agccgagaat ggtgccgagc ccgaccccct
     3781 tcgagacgaa gaggacgaga tcgtgaggca agagggcgcg agcgatcccg gagcgcagct
     3841 cgaccgggct cggaccgagc acgctctgac
//
LOCUS       DASTKS010000170         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_26931,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000170 DASTKS010000000
VERSION     DASTKS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26931"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..801
                     /locus_tag="VFQ35_03590"
     CDS             10..801
                     /locus_tag="VFQ35_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9929738.1"
                     /translation="MATTVAEDRHAPDSSSSTSSSRSDVTFVVKAPTQGDGERIASEA
                     ATLASPFPSVSGPKRRRAFAVAALATGLCGAALWLVFAQPRSSSSALPSTPPAPTARG
                     LALHSPGSTSSDRALTRAGEDSCGELTPGITLCTTKGTEVDRVELGDSERVLYLNEGR
                     AIASVAQQPSGASFRVVTAAGSITATSAVFSVQVDESGTTARVSRGAVALRTSQNDGG
                     RPLLAGQMLRLGTLESSRLPPEEASKDRTILERLGRLSFHPDERE"
     gene            906..1508
                     /locus_tag="VFQ35_03595"
     CDS             906..1508
                     /locus_tag="VFQ35_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929739.1"
                     /translation="MTLTLRDAGSTSSSPIESNAEDIDLVRRVAAGDTGAQRLMVSRL
                     MRRVQRLCGSLARNREDANDASQASLIAILRSASCFRGESSLERWADRIAVRTTLRQL
                     RERRRDSLSLAGCPPQAVYLSADPAIAATEYLESLPEVQRVALILRCGFEYSVEEIAE
                     LTHSSPNTVKDRLKRARVNVRRQLERETLKTSVGVAIRRR"
     gene            1505..2452
                     /locus_tag="VFQ35_03600"
     CDS             1505..2452
                     /locus_tag="VFQ35_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929740.1"
                     /translation="MMDRCQAWINISDRVLLNERVTSAERAFLREHEAVCKTCAREAS
                     AWSDLRPTALYSVPDPDEVEAVIESAAVTTPNHPAFAIMASKPPWLRRAAAALAAAVA
                     LVAAAMFLRPGADDADAASETSSRRVAPAVLGATATPRGVVASAEDTCGEVADGVTVC
                     VTAGSEIPTVALDTARPSLALKRGRVIASLECRAMRTSFGIITEKGSVAGGPLFSVEV
                     AEDGAVTARVLRGNVLVQGAAGERGHALLAGQLLRLGERYSLRLTDDDRERDLSLLER
                     FAPAAFIRQYTSAFGERGSGRTPASFDPEMLRAQRHDAL"
     gene            2567..>3339
                     /locus_tag="VFQ35_03605"
     CDS             2567..>3339
                     /locus_tag="VFQ35_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET9929741.1"
                     /translation="MNALPAPQFDTFEEISSLRRLPVDWPEPGPIELSVHDLPHASAS
                     LEWWSVNCHVETVDGRDLSLFAAFGRERFPMAPGRRDLAIAHSVVWAICDPRQSLYSA
                     KVSVDGLAPALALAKLDATTDTTGSRLDRALREVLMRGQVPAPTQLLSAPVRVDQSSL
                     GLDYGGDTLFKRGAREYFLSLYDDRTKVGCDLFFVPQKRPVRYGANGIARGARDEVMF
                     HYFIPRCSVSGHVTIDRKTRAVGSGTGWLDHEFGFSHEA"
BASE COUNT          567 a         1105 c         1087 g          580 t
ORIGIN      
        1 gttttgcgga tggcgacgac cgtcgcagaa gaccggcacg cgccggattc atcgtcgagc
       61 acctcgagct ctcgaagtga cgtgacgttc gtcgtcaagg ctccgacgca aggcgacggg
      121 gagagaatcg cttccgaagc cgccaccctc gcctcgccct ttcccagcgt atcggggccc
      181 aagcggcggc gcgctttcgc ggtcgcggcg ctggccacag ggctttgcgg cgcggcattg
      241 tggctggtct ttgcgcaacc gaggtcttcg tcgagcgcgt tgcccagcac gccccccgcg
      301 ccgaccgccc gcggcctcgc gcttcacagt cccggttcta ccagttcaga tcgagcattg
      361 actcgcgcag gtgaggacag ctgcggagag ctcacacccg gcattacact ctgtacgacg
      421 aaagggacgg aggtcgaccg agtggagctc ggcgattcgg agcgcgtact ctacttgaac
      481 gaggggcgcg ccatcgcttc tgtcgcgcaa cagccttcgg gtgcttcatt cagagtcgta
      541 acggccgcgg ggagcatcac cgcgacgagc gccgtgttct cggtgcaagt ggacgaaagc
      601 gggactaccg cgcgcgtttc ccgaggcgcg gtcgcactcc gaacgagcca gaatgatggc
      661 ggtcgtcccc ttctggcggg acaaatgctg cgcctcggaa cgctcgaatc gtcccgcctc
      721 ccgccggagg aagcgagcaa agatcgcacc atcctcgagc gcttggggcg actctcgttc
      781 caccccgacg aacgagagtg atcccgaccc tccgagcgcg agccgattag tcagggcccg
      841 tcccgattgc ccgaaatgtg ttcggcaccc gcccgttctc atcgcggctg cccacctgga
      901 ggatcatgac cctaaccctc cgtgacgccg gttcgacctc gtcgtcgccg atagagagca
      961 acgctgagga catcgatctc gtgcggcgag tggccgcagg agacacgggc gctcaacgcc
     1021 tgatggtgag ccgtctgatg cgacgcgttc agcgcttgtg cggttcgctc gctcgcaacc
     1081 gcgaggacgc gaacgatgcg agtcaggcga gcctgatcgc gatattgcgc tcggcgagtt
     1141 gctttcgcgg cgagagcagc ctcgagcgct gggcagaccg catcgcggtg cgaaccaccc
     1201 tgcgacagct gcgggagcga cggcgagact cactgagcct ggccggctgt ccaccgcagg
     1261 cggtgtacct cagcgcagac cccgccatcg ccgcgacgga gtacctcgag tctctgccgg
     1321 aggtgcagcg ggtcgccctg attctgcgtt gcggcttcga gtactcggtc gaagagatcg
     1381 ccgagcttac gcattcttcg ccgaacacgg tcaaggatcg actcaaacgg gctcgcgtca
     1441 acgtgcgtcg tcaactcgag cgagaaacgc tcaagacgtc cgtcggcgtc gccatacgaa
     1501 gaagatgatg gatcgctgtc aggcatggat caacatctcg gatcgcgtgc tgctgaacga
     1561 gcgtgtcacg agcgccgagc gcgcgtttct gagagagcac gaggcggttt gtaagacttg
     1621 cgctcgtgag gcgagtgctt ggagcgacct ccgccccacc gcgctctatt ccgtgccaga
     1681 ccccgacgag gtggaggccg tcatcgagag cgcagcggtt acgacgccca atcaccccgc
     1741 gttcgcaatc atggcgagca agccgccgtg gctgcgccgg gccgcagcgg ctctggcggc
     1801 agccgtggcc ctcgtggctg ctgcgatgtt cctgcgaccc ggcgctgacg acgccgacgc
     1861 ggccagcgaa actagttcgc gtcgcgtcgc gccagcagtt ttgggggcaa ccgcgacccc
     1921 acgcggcgtc gtcgcgagcg ccgaagatac ctgtggcgag gtcgcggatg gcgtcaccgt
     1981 gtgtgtaacc gcgggctccg agattccaac cgttgcgctc gacacagcgc gtcccagcct
     2041 cgcgctgaag cgagggcgcg tcattgcttc actcgagtgc cgcgcgatgc ggacgtcgtt
     2101 cggcatcatc acggagaaag gaagcgtggc gggcggtccc ctgttctcgg tcgaggttgc
     2161 cgaggatgga gccgttaccg ctcgcgtgct gcgcggcaac gtcctggtcc agggagccgc
     2221 aggagagcgt ggccatgcgc tgttggcggg gcagctgctg cggctcggcg agcggtattc
     2281 gctgcggctc acggatgatg atcgcgagcg cgacctctcg ctcttggaac gcttcgcacc
     2341 ggccgccttc atccgacagt acacgagcgc gttcggcgag cgcggctctg gcaggacgcc
     2401 agcgtcgttc gacccggaga tgctgcgtgc gcagcgccac gacgcgctgt gagccgctat
     2461 cacgccgcct cacccgagca gcgcagagcg cgccccagcg cgcaggcggc gcgactgtcg
     2521 ccacccgaat tcacgaatgg cggacactcg aactgcacca gcatccatga acgctctacc
     2581 tgccccccag tttgacacat tcgaagaaat ctcttcgttg cgccggctgc cggtcgattg
     2641 gcccgaacct ggcccaatcg agttgagcgt acacgatcta ccgcacgcct cggcttctct
     2701 cgagtggtgg agcgtcaatt gccacgtaga gaccgtggac ggccgcgacc tgtctctgtt
     2761 cgccgcgttc gggcgcgaac gctttccgat ggctcccgga cggcgtgacc tagcgatcgc
     2821 acattcggtt gtatgggcga tttgcgaccc gcgtcagtcg ctatattcgg ccaaggtgtc
     2881 tgtcgatggg ttggcgcccg cgctcgctct cgccaagctc gacgcaacca cggacaccac
     2941 cggctcgcgt ctcgaccggg cgctccgaga agtgctgatg cgcggtcagg taccggcacc
     3001 gacgcagctc ttgagcgcac cagtgcgagt cgatcagtct tcgctgggtc tcgactacgg
     3061 gggggacacc ctattcaagc gcggagcgcg cgagtacttc ctctcacttt acgacgaccg
     3121 gacgaaggtc ggctgtgacc tctttttcgt cccgcagaag cgtccagttc gctacggtgc
     3181 caacggcatc gcgcggggcg ctcgagacga agtcatgttc cactacttca tcccgcgctg
     3241 cagcgtttct ggccacgtga cgatcgaccg aaagacgcga gccgtcggat ccggaacggg
     3301 ctggttggat cacgagttcg gcttctcaca tgaagccaa
//
LOCUS       DASTKS010000171         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1811128, whole genome shotgun sequence.
ACCESSION   DASTKS010000171 DASTKS010000000
VERSION     DASTKS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811128"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFQ35_03610"
     CDS             complement(<1..590)
                     /locus_tag="VFQ35_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9929742.1"
                     /translation="MSSSSGSQALTFLKRHARAIVASVALAGGLGWILHKGALPLTPP
                     PGTLDNVIWFKVALMTVGLLASMLLRFARYGLLIAPIARVPMRRIMSISSIAIGLITF
                     LPFRLGEFVRPAMLREKGKISGWAVTGTVGAERIMDGLVFGAMLMTGLVFAKPHEPLP
                     DHIGDLPVPAALVPRAAQLFTLVFAVAFVVMAAFFW"
     gene            complement(587..1651)
                     /locus_tag="VFQ35_03615"
     CDS             complement(587..1651)
                     /locus_tag="VFQ35_03615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9929743.1"
                     /translation="MNDKSPAARGKELSALSVENELLDLSVVVPVYNEEESLPHLVEE
                     LHRALAPTGLAYELVLVDDGSRDRSFTILKEMAKRDGSLHIVRFRRNFGQTAAMQAGL
                     DVSRGAKVVTIDADLQNDPADIPAMLQKLDEGYDMVAGWRRDRKDAFINRKLPSLIAN
                     RIISLATKVKLHDYGCTLKAMTSEVAKELRLYGEMHRFIPAVASWVGVRILEMPVNHR
                     ARQFGTSKYGIGRTLRVVLDLMTVRFMQTYLVRPMQVFGLAGLASLGAGVLLCAWLVL
                     EKLVFSARLADRPLLLLGVLLIVVGVQLLSLGLVADVVSRTYHESQGKKPYYVRERVV
                     GPGSTRASHVSAARVAHEVS"
     gene            1952..2836
                     /locus_tag="VFQ35_03620"
     CDS             1952..2836
                     /locus_tag="VFQ35_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HET9929744.1"
                     /translation="MKQRSVRVGLVQMSTTADKAQNLQKAADRIVEAGRRGAELVCLQ
                     ELFASPYFCQREDAELFDLAEPIPGPTIEAMARAARQAKVSVVVPLFERRAPGLYHNS
                     LVVLGPDGESLGTYRKMHIPDDPLFYEKYYFTPGDLGFKVFETERAKLGTLICWDQWY
                     PEAARLTALAGAELLVYPTAIGWHPSEKAETGAAQVSAWQTMQRAHAIANGCFVIAVN
                     RVGHEGPSDGGLEFWGHSFVADPFGIVLAEAGEGEEILVVECDLTRMETVRRNWPFLR
                     DRRIDAYGALTQRFLDPR"
     gene            2849..4021
                     /locus_tag="VFQ35_03625"
     CDS             2849..4021
                     /locus_tag="VFQ35_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET9929745.1"
                     /translation="MPKTSKRARRAPGPEMATQPNETPRSLGYRMPAEWEPHAATWLA
                     FPHEASDWPGKFEVVPWIFAELAKKLTEGERVRLIVRDAAMKKHAQRVFQQTGVDMSQ
                     VDFLSAPTDRSWTRDFVPSFVLKERARVPRRARELGARGADGVAAVKWRFNGWARYPN
                     HRRDEAAGTLVKDWLGVPAFTPEAAGAGRRKAARLVLEGGAIDVDGEGTLLTTEECLL
                     DGKQARNRELGKEGTERALSANLGIDKVLWLKQGIAGDDTGGHVDDFARFAGPARVVL
                     CEERRKSDPNHRILESARERLEGARDARGRRVQVVRLPMPSPLSFAGQRLPASYANFY
                     VCNAYVLVPTFNDPADRIALGILSELFTDRPVVGVHAVDLVLGLGTIHCSTQQEPL"
BASE COUNT          779 a         1299 c         1311 g          636 t
ORIGIN      
        1 caccaaaaga aggcggccat gacgacgaag gcgaccgcaa aaaccagcgt gaagagctgc
       61 gcggcgcggg gaaccaaggc cgccggcacc ggcaggtcac cgatgtgatc cggaagcggc
      121 tcgtgcggct tcgcgaacac gaggcccgtc atcaacatcg cgccgaagac gagcccgtcc
      181 atgatgcgct cggcgccgac agtcccggtg accgcccacc cagaaatctt ccccttttcg
      241 cgcaacatcg cggggcgcac gaactctccg aggcgaaacg gcaagaacgt gatgagcccg
      301 atcgcgatgc tcgaaatgct catgatgcgg cgcatcggca cgcgtgcgat gggcgcgatg
      361 aggaggccat accgcgcgaa gcgcagcaac atgctcgcca agagcccaac cgtcatcaag
      421 gcgaccttga accagatgac gttgtcgagc gtgcccggcg gcggcgtcag cggcagcgcg
      481 cccttgtgca aaatccaccc gaggcctccc gcaagcgcga ccgaagccac gatggcgcgc
      541 gcgtgtcgct tgaggaaggt cagcgcttgc gatcccgaac tcgaactcat gacacctcgt
      601 gcgcgacgcg cgcggccgaa acgtgcgagg cacgtgtcga accgggaccg acgacgcgct
      661 ctcgaacgta ataaggcttt ttgccctgcg actcgtgata ggtgcgcgac accacgtccg
      721 cgaccaagcc gagcgagagc agctgcacgc cgacgacgat gagcagcacc ccgaggagca
      781 agagcgggcg gtcggcgagg cgcgccgaga atacgagctt ttcgagcacc agccacgcgc
      841 agagcaggac cccggccccg agcgacgcga gccccgcgag gccgaacacc tgcatggggc
      901 ggacgaggta cgtctgcatg aatcgcaccg tcatcaagtc gaggacgacg cgcagggtgc
      961 gaccgatacc gtatttcgaa gtgccgaact gacgcgcgcg gtggttgacc ggcatctcca
     1021 agatgcgcac gccgacccag ctcgccaccg ccgggatgaa acgatgcatc tcgccataca
     1081 ggcggagctc tttcgcgacc tcggaggtca tcgccttcaa ggtgcagccg taatcgtgga
     1141 gcttcacctt cgtcgcgagg ctaatgattc gattcgcgat cagggacggc agcttccggt
     1201 tgatgaaggc gtctttacga tcgcgacgcc accccgcgac catgtcgtag ccctcgtcga
     1261 gcttctgcag catcgctggg atgtccgcgg ggtcgttctg gagatcggcg tcgatagtga
     1321 cgaccttggc gccgcgagaa acgtcgagcc ccgcttgcat cgccgccgtt tgtccaaaat
     1381 tcctacgaaa gcggactatg tgcaggctgc catcgcgctt agccatctct ttcagaatgg
     1441 taaagcttcg gtcccgcgat ccgtcatcga cgagaactaa ttcgtaggcg agacccgtag
     1501 gagcgagcgc gcgatgcaac tcttcgacga ggtgaggcaa gctctcttct tcgttgtaga
     1561 cgggcacgac gaccgagagg tccagaagct cgttttccac actcaacgcg gacaactcct
     1621 tgccacgggc ggccggggac ttatcgttca tcacgtctcg cgaccaggtg aaccttcggc
     1681 tcggtcgatc ctcgaagagt cggcataaaa agggttcatt tccacgtctt cttgcgaaaa
     1741 aagttcgttc cgatgctcga ggacgagacc gtggcccttt agcacgcccc cggactggac
     1801 ggcgtttgac gcgcgaacgc cacgcttttg caggagaaaa gccatggttt tggaggggcc
     1861 tcccagccgg acgaagccgg cggcagcggt aggcggcggc gcggcggtct ccttttgaaa
     1921 gctccagaag tcccgctacg ctccggccgc catgaaacaa cgaagcgtgc gggtcggcct
     1981 cgtgcagatg tcgaccactg cggacaaagc acagaacctc cagaaggccg ccgatcgcat
     2041 cgtcgaagcc ggccggcgcg gcgccgagct cgtatgcctg caagagctct tcgcgtcgcc
     2101 gtacttttgt cagcgtgagg acgccgagct gttcgacttg gccgagccga tccccggacc
     2161 cacgatcgag gcgatggctc gcgcggcccg gcaggcgaaa gtcagcgtgg tagtcccgct
     2221 gttcgagcgc cgcgccccgg ggctctatca caacagcctg gtcgtgctcg gacccgacgg
     2281 cgagtcgctc ggcacgtacc ggaagatgca catccccgac gacccgctgt tttacgagaa
     2341 gtactatttt acgcccgggg acctcggctt caaggtgttc gagaccgagc gagcgaagct
     2401 tggcacgctc atttgttggg accagtggta cccggaggca gcgcggctca cggcgctcgc
     2461 gggggccgag ctcctcgtct acccgaccgc gatcggatgg catccctccg aaaaagcgga
     2521 gaccggtgcc gcacaagtga gcgcctggca gacgatgcag cgcgcacacg caatcgcgaa
     2581 cggctgcttc gtcatcgccg tcaaccgagt cgggcacgaa gggccgagcg acggcgggct
     2641 cgagttctgg ggccactcct tcgtcgcgga tccgttcggg atcgtgctcg ccgaggccgg
     2701 cgaaggcgaa gaaattctgg tcgtggagtg cgacttgacg cgaatggaga cggtgcgacg
     2761 caactggccg ttcctgcgcg atcggcgcat cgacgcgtac ggcgccctca cccaacgctt
     2821 cctcgacccg cgctgaccga gccgaaagat gccgaaaacg tcgaagcgcg cccgtcgcgc
     2881 accgggcccc gaaatggcga ctcagccgaa cgagactccg cgaagtctcg ggtatcgcat
     2941 gccggcggag tgggagccgc acgcggccac gtggctcgcg tttccgcacg aagcgagcga
     3001 ctggcccggc aagttcgagg tcgtgccgtg gatcttcgcc gagctcgcga agaagctgac
     3061 cgaaggcgag cgcgtgcggc tcatcgtgcg cgacgcggcg atgaagaagc acgcgcagcg
     3121 cgtgttccag cagaccggcg tcgacatgtc gcaggtcgat ttcttgtcgg cgccgaccga
     3181 tcgctcttgg acgcgcgatt tcgtcccgag cttcgtgctc aaagagcgag cgcgtgtccc
     3241 tcggcgtgcc cgagagctcg gtgcgcgcgg cgcagacggc gtcgcggccg tcaaatggcg
     3301 attcaacggc tgggcccggt acccaaacca ccgccgagac gaggcggcgg gaacgctggt
     3361 gaaggactgg ctcggtgtgc cagcgttcac tcccgaagcg gcaggcgcag ggcggcgcaa
     3421 agcagcgcgc ctggtgctcg aaggtggagc tatcgacgtc gatggcgaag gcacgctgct
     3481 caccaccgaa gagtgcttgt tggacggcaa gcaggcgcgg aaccgggagc tcggcaaaga
     3541 gggcacggag cgagcactct cggcgaacct cggtatcgat aaggtcctgt ggctgaagca
     3601 gggaatcgcc ggagacgaca ccggcgggca cgtcgacgat ttcgctcgct tcgccggacc
     3661 tgcgcgtgtc gtgctctgtg aggagcgccg caagagcgat ccgaatcacc gcattttgga
     3721 gtctgcgcgt gaacgcctcg aaggcgcgcg cgacgcccgc ggacgcagag ttcaggtcgt
     3781 tcggctaccg atgccttctc cactttcgtt cgccgggcaa cgcctgccgg cgagttatgc
     3841 aaacttctac gtctgcaacg cctacgtgct cgtaccgacg ttcaacgacc ccgccgatcg
     3901 catcgcgctc ggcatcttgt cggagctctt cacggaccgg cccgtggtcg gtgtgcacgc
     3961 cgtcgaccta gtgctcggac tcggaacgat ccactgcagc acgcaacagg agccgctcta
     4021 gcttc
//
LOCUS       DASTKS010000172         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_27343,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000172 DASTKS010000000
VERSION     DASTKS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27343"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFQ35_03630"
     CDS             <1..1037
                     /locus_tag="VFQ35_03630"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET9929746.1"
                     /translation="PLKSDFAGTVGTRLPLANAPIGTASCPELAAAVSNAGALGMMAV
                     SFLSPSGVRAAIRRVRELTDRPFAANLLLEWDQHERLEVCLEEGVELISLFWGDPAPY
                     AERIRRGGARFIQTVGSVEEAVHAVDAGASAIIAQGWEAGGHVRGEISTLALVPQVVD
                     AVRPTPVAAAGGIANGRGVAAAMMLGASAAVLGTRFVCSKEAAVPPIYQELVQKADSS
                     QTVFGETFDKGWPDSNMRTLLNSTLRRWIEAGRPAIGSRPGETDIVARAGDGSDIRRY
                     SVTQPATGMKGDLEALALYAGQSAGMVNDVRSAGEIVRDLWREAVTALRGAEPLMRKG
                     SGHAALRRNP"
     gene            1095..1718
                     /locus_tag="VFQ35_03635"
     CDS             1095..1718
                     /locus_tag="VFQ35_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929747.1"
                     /translation="MLGVRDATVDDSEAFVNYWHYSGDQIKEALRIDLVRLGTPEDTR
                     NRFLRMLRRPGVRSPSVVFSLTLDDEVIGYTNVNMHGPLENYPHFHTYVHTDTRVKKK
                     IAEALAHTPRADTSKTGAGIAAVMIGFGIGSITKHLTHIERLVLQTRVTSVAINRALD
                     LYMPADETRFIENPDGVASAGEFHMRYVPRSRAAVFLERAESLARGG"
     gene            1723..>2717
                     /locus_tag="VFQ35_03640"
     CDS             1723..>2717
                     /locus_tag="VFQ35_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929748.1"
                     /translation="MTMETDFSLDSRRQIIDHTASFDDAVVFLGLVAAGRIKSEECAE
                     AFHKGKRIERFIGQVVSQRLIRTEEWTALQQLWYRLRVPFGVASLGYWQLFCVAYGQT
                     GQIAAACDRAAAWIEAIAGAPPESRVRVYSMQTGMSAERGVVAMQHDMTSLDCLARSA
                     FDELPLVPLQSADELALLTRDGTRVVLTDVARDLESAASNRLPQPSRSVASVREFCGE
                     RPFSVARGSREMREFWCELTPEDRTYILVHLEQNIQALEIVRFSYGNQVMDNESQAAV
                     LNKCRATRPEEWSQRELVLASLLWGWTQAGFMVHEFNQSELSLHGFRIFLERRIH"
BASE COUNT          498 a          833 c          905 g          481 t
ORIGIN      
        1 gcccactcaa gtcggatttc gctggcacgg tggggacgcg gttgcctctc gccaacgccc
       61 cgatcggtac ggcttcctgc cctgagctcg cggcggcggt ttcgaacgcg ggagctctcg
      121 ggatgatggc ggtgagcttc ctgagcccgt ccggggtgcg cgccgcgatt cgccgggtgc
      181 gcgagctgac ggatcggccc ttcgccgcga atctactgct cgaatgggat cagcacgaga
      241 gactcgaagt ttgtttggaa gagggagtgg agctcatctc cctgttctgg ggtgatcctg
      301 cgccgtacgc ggagcgaatt cgacgcggcg gcgcgcgctt cattcagacc gtcggcagcg
      361 tggaagaggc cgttcacgcc gtggacgccg gggcgtcggc gatcatcgcc cagggctggg
      421 aggccggcgg ccacgtgcgc ggcgagatct cgacgctcgc gctcgtgcca caggtggtgg
      481 atgccgtccg gccgactccg gtcgcagcgg cggggggcat cgccaacggc cgtggtgtcg
      541 ccgcagcgat gatgctcggc gcgagcgcgg ccgtgctcgg aacccgcttc gtgtgctcga
      601 aagaagcggc cgttccgccg atctaccaag agctcgtcca gaaggcagat agcagccaga
      661 ccgtgttcgg cgagacgttc gacaaaggtt ggcccgattc caacatgcga acgctgctga
      721 acagtacact acggcgttgg atcgaggcgg ggcgtcccgc gatcggtagc aggcctggag
      781 agacggacat cgtggctcgg gcgggggacg gttcggacat tcgacggtac agcgtcacgc
      841 agcctgcgac tgggatgaag ggagacctcg aggcactcgc cctctacgct gggcaaagcg
      901 cgggtatggt gaacgacgtg cgaagcgcgg gcgaaatcgt gcgagatctt tggcgcgaag
      961 ccgtgacggc gcttcgaggc gccgaaccac tgatgcgaaa gggctctgga catgcagcac
     1021 tacgaagaaa tccataagga cgactagggt ggggggccgg gcgcttctcc gcccaaatac
     1081 gggcgcgcgt cggcgtgctc ggtgttcgcg acgcgaccgt cgacgacagc gaggcgttcg
     1141 tcaactactg gcactacagc ggggaccaga tcaaagaggc gctgcgcatc gacctcgtgc
     1201 ggctcggcac gcccgaagac acgaggaatc gcttcctgcg catgctgcgt cgccccggcg
     1261 tgcgctcccc ctcggtggtg ttctcgctga cgctcgacga cgaggtgatc ggttacacga
     1321 acgtgaacat gcacgggccg ctcgaaaact acccgcactt tcacacgtat gtgcataccg
     1381 acacgagggt gaagaagaag atcgccgagg ccctcgccca cacccctcga gccgacacca
     1441 gcaagacggg ggccggcatc gcggcagtga tgattggctt cggaatcggc tcgattacca
     1501 agcacctcac gcatatcgaa cgattggtac tgcaaacgcg tgtcactagc gttgcgatca
     1561 accgcgcgct cgacctgtac atgccagcgg acgaaacccg attcatcgag aacccggacg
     1621 gcgtcgcctc cgccggcgaa tttcacatgc gctacgttcc gcgctcgagg gcggcagtat
     1681 ttctcgagcg cgccgagtcc ctagcacggg gaggatgact aaatgacgat ggagactgat
     1741 ttttcactcg attcgcgccg acaaatcatc gaccacactg cttcgttcga cgacgcggtg
     1801 gtcttcttgg ggctggtcgc ggccggcaga atcaaaagcg aggagtgcgc cgaagcgttt
     1861 cacaagggaa agcgcatcga gcgtttcatt ggtcaggtgg tttctcagcg cctgatccgg
     1921 acggaagagt ggacggcgct gcagcaactt tggtaccggc tacgagtacc attcggggtc
     1981 gcttcgctcg gctattggca gctgttttgc gtcgcctacg gtcaaaccgg gcagattgcg
     2041 gccgcatgcg accgcgccgc ggcttggatt gaagccatcg ccggagcgcc gcccgaatcg
     2101 cgagtacgcg tgtattcgat gcagactggc atgagcgccg agcggggcgt cgttgcgatg
     2161 cagcacgaca tgacttcgct cgactgcctc gctcgctccg cattcgacga actgccgctc
     2221 gtcccgctgc aatccgccga cgagctcgcg ttgctcactc gggatggcac acgcgtcgtg
     2281 ttgaccgacg tcgctcgtga cctggagagc gctgcttcga ataggctccc gcagccgagt
     2341 cgttcggtcg cgagcgtgcg agagttttgc ggtgagcggc cgttctccgt ggcgcgtggc
     2401 tcgagagaga tgcgcgagtt ctggtgcgag ctcacgcccg aggaccgcac atacattctc
     2461 gtgcacctcg agcaaaacat ccaagccctc gagatcgttc gcttcagcta cggcaaccaa
     2521 gtgatggaca acgagagcca agctgcggtt ctcaacaagt gccgcgcgac gcgccccgaa
     2581 gagtggtcgc agcgagagct cgtgctggct tccttgctct ggggttggac ccaagccggc
     2641 ttcatggtgc acgagttcaa ccaatccgag ctcagcttgc atgggtttcg gatcttcctc
     2701 gagcgccgca tccacga
//
LOCUS       DASTKS010000173         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_473755, whole genome shotgun sequence.
ACCESSION   DASTKS010000173 DASTKS010000000
VERSION     DASTKS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473755"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(645..2138)
                     /locus_tag="VFQ35_03645"
     CDS             complement(645..2138)
                     /locus_tag="VFQ35_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9929749.1"
                     /translation="MTRPFDVLVIGAGFGGLASALTLGELGARVCLCEMLKYPGGCAS
                     TFTKAGFRFDAGATLLSGLAEPQLFGRWLSKYSPETRIEWSDRLIDFRSGPLRLDVGR
                     SRERLVDALGSLPGAPEQRIREFFSVQKVVADALWPLLDDATLLPPISAPMLARHARR
                     LREYARIAPYLGRPLVNVMRAHRVDDFLPLRLYVDALCQITVQCGAAEAEAPIALSAM
                     DYVFRGTAHVHGGVGNLAEALLAAARRVGVEVRMPNRVSALRFDPRYGYRAITRGGEI
                     RARAVVSNLLPSAVSRLLGDPTLPPELRDMQTAVDDGWGAAMVYGVASDPTGAPPEAH
                     HLQLVDDERAALCEGNHVFLSISGRDETQRAPLGKRTFTASTHVPLSAVRAIGSNDEV
                     GRYISEIQSRMLRTVRRRAPLWADAVERFWTASPRTFERFVGRPEGAVGGARRVAGLH
                     NYHHMGPSELRPNLWLVGDTVFPGQSALATAVGGVRTAHVTARRLNI"
     gene            2248..>2510
                     /locus_tag="VFQ35_03650"
     CDS             2248..>2510
                     /locus_tag="VFQ35_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HET9929750.1"
                     /translation="MHTPTPEFLFEVFYDGDCPLCVREIAMLRRRDKAQRIRFTNIAE
                     PEFDPSALGVTWETLMKRIHGRLPNGELVEGVEVFRQLYAAVG"
BASE COUNT          478 a          807 c          816 g          409 t
ORIGIN      
        1 gccctgacgt tgcgcggcgg ggcgtcctgc atcgagttcg cggggctgcg actggaggtc
       61 aacttctttg cggtcgaaag cgacctcctc ttgccggtga gctcgcacat cacccgcaat
      121 caggcccggc gcgcggaacg ctattcgctc atgctacccg atccactgag cgcgactcgc
      181 gtcgcgttcc aaccttcggt gcgaacgccg ggatggctcc cgaagagagc tccgaagtca
      241 ggcgccacgc gtcctcgtga tgatgagctc gcgtgcccaa cccctgcccc tcgtgctctg
      301 cggagagaag agctcgcgaa cgagatgcgg gtgttcagcg gcgaatgcct cgaattccct
      361 tcggcgcgcg aaatcggcgc tctggccgat ccccgatcgg ttgcgatcga gcgacgggaa
      421 gtcctcgaga tcaacggtga agagcgccgg gggcgccgtc gccgaatctc tccctcgcgg
      481 ttggttgcgg cggctagagc gtggttggca aagtgctttc gaccgcgtcg agtctcattg
      541 gagggcgtca cgggagcaca atcgatcacg cgaagcgaac gatgagggtt ccagcgcggt
      601 cgtctgatcc ggacacgagc tatcggtgcc ggcgcgggtg cgattcaaat gttcaatcgc
      661 ctcgcggtga cgtgcgcagt tcggacgccc ccaacggcag tcgcgagcgc gctctgacct
      721 gggaacaccg tatcccccac gagccacaag ttggggcgga gctcgctcgg tcccatatgg
      781 tgatagttgt ggaggcctgc gacgcggcgg gctccaccga ccgctccctc cggccgcccc
      841 acgaagcgct cgaacgtgcg gggggacgcc gtccaaaagc gctcgaccgc atcggcccac
      901 agtggcgcgc gccgtcgcac ggtgcgcagc atgcgcgact ggatttcgga gatgtagcgg
      961 ccgacttcgt cgttcgaacc gatggcacga acagcggaca ggggcacgtg cgtcgacgcg
     1021 gtgaaggttc gctttccgag gggcgcgcgc tgtgtttcgt cgcgtccgct gatcgataga
     1081 aagacgtgat tgccttcgca cagggcggct cgctcatcgt cgacgagctg aaggtgatgc
     1141 gcctcgggag gcgctccggt ggggtcgctc gcaacgccat agaccatggc tgcgccccac
     1201 ccgtcgtcca ccgctgtctg catatctcgg agctcgggcg gcaaagtcgg gtccccgagc
     1261 agccgcgaaa cggccgacgg cagaaggttc gagaccacgg cgcgagcacg aatctcgccg
     1321 ccgcgcgtga tcgcacgata accgtacctc gggtcgaagc ggagcgcgct cacgcggttc
     1381 ggcatccgaa cctcgacgcc cactcggcga gccgcggcga gcagtgcttc ggccaagttg
     1441 ccgacaccgc cgtgaacgtg cgccgtgccg cggaaaacgt agtccatcgc cgaaagcgcg
     1501 atgggtgctt cggcttcggc cgcgccgcat tgcaccgtga tttggcagag cgcgtcgacg
     1561 taaaggcgaa gcggtagaaa atcgtcgacg cggtgagcgc gcatgacgtt caccaagggc
     1621 cggccgaggt acggagcgat gcgcgcatac tcacggagcc gacgggcatg gcgcgcaagc
     1681 atcggcgcgc tgattggcgg gagcagagtc gcgtcatcca acagtggcca aagcgcgtcg
     1741 gcaacgacct tctgcacgga gaagaattca cgaatgcgct gctccggtgc gcctggcaac
     1801 gaccctaagg cgtcaaccaa gcgctctcgc gaccgcccca cgtcgagtcg aagcggaccg
     1861 cttctgaagt cgatcaatcg atcgctccat tcgatgcgcg tttcgggaga atacttggac
     1921 agccagcgtc cgaacagctg cggctcggcg agcccactca gcaaagtcgc acccgcatcg
     1981 aaacggaaac ctgctttggt gaacgtgctg gcgcaccctc ccggatactt gagcatctcg
     2041 cagaggcaca ctcgtgcgcc tagctccccc aaggtgagag cagatgcgag gccgccaaaa
     2101 cccgcgccaa tcacgagcac atcgaagggg cgggtcatcc cgcaaggctc cctaggaggg
     2161 ttgacataag ttggacagaa cctagacata acttagacag cacgccgcgt aaccgcaagg
     2221 gacgaggcgt catgtggaag agttcgcatg cacacaccca ccccagagtt cctgttcgag
     2281 gtcttctacg acggagactg tccgctctgc gtgcgagaga tcgcaatgct ccgccgccgt
     2341 gacaaggcac aacgcatccg tttcacgaac atcgcagaac ccgagttcga cccgagcgcg
     2401 ctcggcgtga cttgggaaac cttgatgaag cgcattcatg gccgcttgcc gaacggcgag
     2461 ctggtggaag gcgtcgaagt cttccgtcaa ctgtacgccg ccgtcggctt
//
LOCUS       DASTKS010000174         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_918793, whole genome shotgun sequence.
ACCESSION   DASTKS010000174 DASTKS010000000
VERSION     DASTKS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918793"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1029)
                     /locus_tag="VFQ35_03655"
     CDS             complement(106..1029)
                     /locus_tag="VFQ35_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929751.1"
                     /translation="MSEFWSKLLAKPWLRVQACIASMGAILLAGSATYATYRVKDSAV
                     RLGSEIFRAVELEGGEVIEFNGARFHFAANVVTDELDAVAERAARLCEQDAAELASEL
                     APALSRLPAAAMRGHELDARKLLTLESAKAPSVREIGCFRRPTGSARGFMQRVKRFAE
                     TGDFSEFGTMSYVRVERHGDKTLVRALWSEGRLAVSSLFPAEGDVPGADLRDVPRPRT
                     STRILAARIVGTDRRVVGYDTELGRAELNEFYGRELLRLGWKELDLGAKEPSNERPSR
                     AFERGERRALLALTPSAHGTSATFVELVANR"
     gene            complement(1050..1781)
                     /locus_tag="VFQ35_03660"
     CDS             complement(1050..1781)
                     /locus_tag="VFQ35_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929752.1"
                     /translation="MEKRVQVLRRRRFQRGAVAVEGIIVAMLLVGIVLGTWFMHHVLV
                     AKWQTMQQSRSAAWISALQGCVDDSASHLYEPLHEKSKDPNVKVCDDPANCEGSSVEG
                     LDNGGEGTPPGWFPQSDGKDSSFSKLVEVISFAPRTLTTRRQFSCNEKPSGELQFGSS
                     GALDGVADIARKIVDEEVQPDESAKKCQRRYGIPLQWHDVSCDEGLYKFGAGGADPLH
                     WYTRKVDLDIPPPPADLTPPPPPQK"
     gene            complement(1789..3384)
                     /locus_tag="VFQ35_03665"
     CDS             complement(1789..3384)
                     /locus_tag="VFQ35_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929753.1"
                     /translation="MNLGKLLHDRRGGIAVMGILMGAVLVAVLFHLTNCGFGILWREN
                     AQDAADATAYEAALWNARGMNVIVAVNIFIALVMAILIFWRMVILFMMALTVLLAIAC
                     PFEPEFCEFVPLTANAARAMIQQDERVAGRVYQVISMLHDAQKIVAAATPVMGTLSSV
                     VEAKQYDADFGIGFGTQLIPTVGFVKGKKPDKSCSQKASRWSSKTGSTGDLLGKPISL
                     PVVEADDLSKLCEEGGKMQFVVLWYIVGKVGEKLGIGFLKDLGSQQGGGEFGKAWGSI
                     TGHASDFFCGDFMGIVHEVADDAIDNKCGKDNDCRDKANKGKNKDPLPDPKKSSSIDS
                     SQVRWTRVWDLAANGNLFMQSWSYVKIDRKQIEPLDKILRVADLFRRSTSSGDGVTKE
                     ASTYAQAEMYFDCSSQWSDASCQELAPWAMRWRARLRRVHDPVDMIASDLEATMVSAL
                     YGGLEGHFGGAVASKFAPLTQLLSEHGGRLIQIQTSVDFRSERDQLVRNLPGVSGDGS
                     DTARNAASWVSEQAENGAAEMIH"
     gene            complement(3381..>4025)
                     /locus_tag="VFQ35_03670"
     CDS             complement(3381..>4025)
                     /locus_tag="VFQ35_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929754.1"
                     /translation="DPHIPARPLHKPEAAPEMNHFQETRVFDLSDLGLELPEVPAKAQ
                     APAAKLRLAPPPQLKPEELTEARRLEVKSLDEELIEAFEGEFAELEPTPTQAPQPKLK
                     LAAYSLLGVAAVISGIGMLNPAAAETEARAATPQRAKGAPAASAAVRAPSRPVEPTLS
                     GPPSSKRAADYYATGDWMNALAEYRALAASSADPAYSVIVRALERRVARAQVKR"
BASE COUNT          674 a         1341 c         1267 g          743 t
ORIGIN      
        1 ctccattgag accgagcgtg ggtcgcgcgc gctctcgggt aggcgttgcg gtggtgccga
       61 ttgcggtgtt gttcatcagc ggcagcggtg acatggggtc gagcctcaac ggttcgccac
      121 gagttccacg aaggtcgcgg aggttccatg cgcgctcggc gtcagcgcga gcaaggcgcg
      181 ccgctcgccg cgttcgaagg cgcgcgaagg ccgctcgttc gagggctcct tcgcgccgag
      241 gtcgagctcc ttccaaccga gccggaggag ctcgcgaccg tagaactcgt tcagctccgc
      301 gcgcccgagc tccgtgtcgt agccgacgac acgccggtcc gtacccacga tgcgcgccgc
      361 gaggatccgc gtcgaggtcc gcggacgtgg cacgtcgcgc aagtccgcac ccggcacgtc
      421 gccttccgcc ggaaagagac tcgagaccgc gaggcgacct tcgctccaca aggctcgcac
      481 caaggtcttg tcgccgtggc gctcgacgcg gacgtaagac atcgtgccaa actcggaaaa
      541 gtctccggtc tccgcgaagc gtttcacccg ttgcatgaag cctcttgcgc tgccggtcgg
      601 tcgccggaag cagccgatct cgcggaccga aggagccttc gcgctctcga gagtcagcaa
      661 tttgcgcgca tcgagctcgt gacccctcat cgcggccgcg gggaggcgcg acagggccgg
      721 cgcaagctcc gacgcgagct ctgcggcatc ttgctcgcag agccgggccg cgcgctccgc
      781 taccgcgtcg agttcgtcgg tgacgacgtt cgcggcaaag tgaaagcgtg ccccgttgaa
      841 ctcgatgact tcgccgccct cgagctcgac agctcggaag atctccgaac ccaacctcac
      901 cgcagagtct ttgacgcggt aggtcgcgta ggtcgcgctc ccagccagga gaatcgcgcc
      961 catcgaagcg atgcacgcct gaactcggag ccagggcttg gctaacaact tcgaccaaaa
     1021 ctcggacatc ggagtctgcc tttcgctagt cacttctggg gcggtggagg cggcgtcaaa
     1081 tcggcgggag gcggcgggat gtcgaggtcc actttgcgtg tgtaccaatg cagtggatcc
     1141 gccccgcccg cgccgaactt gtacaggcct tcgtcgcacg agacgtcgtg ccactggagc
     1201 gggatgccgt aacggcgctg acacttcttc gcggactcgt cgggctgtac ttcctcgtcg
     1261 acgatcttcc tggcgatgtc ggcgacacca tcgagcgcgc cgctcgagcc aaactgcagc
     1321 tcaccgctcg gcttttcgtt gcaggaaaac tgacgtcgcg tcgtgagcgt tcgcggcgcg
     1381 aacgagatga cctccacgag cttcgaaaac gaggagtctt tgccgtcgct ttggggaaac
     1441 cagcccggcg gagtcccttc gccaccgttg tcgagccctt cgaccgagct gccttcgcaa
     1501 ttggcaggat cgtcgcagac cttgacgttc ggatctttgc tcttttcgtg caacggttcg
     1561 tagaggtgag acgcgctgtc gtccacgcag ccctgcagcg ccgagatcca cgccgccgaa
     1621 cggctctgct gcatggtttg ccacttcgcg acgagcacgt ggtgcatgaa ccaagtgccg
     1681 agcacgatgc cgacgagcag catcgcgacg atgatgccct cgaccgccac ggcgccgcgt
     1741 tgaaagcgcc tccgtctgag aacttgtacg cgcttttcca tcgacgcctc agtgaatcat
     1801 ctcggccgcg ccgttctcgg cctgctccga gacccagctc gcggcgttac gcgcggtgtc
     1861 ggagccatcg ccagaaaccc ctggcaagtt gcgcaccaat tggtcgcgct cactgcggaa
     1921 gtcgaccgac gtctgaatct ggatcaagcg gccgccgtgc tcgctgagca gctgcgtcag
     1981 cggtgcgaac ttgctcgcga ccgcgccgcc gaagtgaccc tcgagcccac cgtagagcgc
     2041 gctcaccatc gtcgcctcca ggtcggaggc gatcatgtcg actggatcgt ggacgcgccg
     2101 tagccgcgct cgccagcgca tagcccacgg cgcaagctcc tggcagctcg cgtcgctcca
     2161 ctgggaagag caatcgaagt acatttcggc ctgagcgtag gtgctcgcct ctttggtgac
     2221 cccgtctcca gaagacgtgg agcgccggaa caggtccgcg acgcgcaaga tcttatcgag
     2281 gggctcgatc tgcttgcggt cgattttgac gtaactccag ctttgcatga acaggttgcc
     2341 gttcgccgcg agatcccaaa cccgcgtcca gcgcacctgg ctgctatcga tgctcgacga
     2401 cttcttgggg tccgggagcg gatccttgtt cttccccttg ttcgccttgt cgcgacagtc
     2461 gttgtccttg ccgcacttgt tgtcgatggc gtcgtccgcg acctcgtgca cgatgcccat
     2521 gaagtcgccg caaaagaagt cgctcgcgtg gccggtgatc gagccccacg cttttccgaa
     2581 ttcgcctccg ccctgctggc ttccgaggtc cttcaagaag ccgatgccga gcttctcgcc
     2641 gaccttgccg acgatgtacc agagcacgac gaactgcatc ttgccgccct cttcgcagag
     2701 cttcgagagg tcgtcggctt cgacgacggg caagctgatt ggtttgccca gcaagtcacc
     2761 agtgctcccg gtcttgctgc tccagcgact cgccttctgt gagcagctct tgtccggctt
     2821 cttgcctttg acgaaaccca cggtggggat gagctgcgtg ccgaagccga taccgaagtc
     2881 ggcgtcgtac tgcttggcct cgacgaccga gctcagcgtg cccatcaccg gcgttgccgc
     2941 cgccacgatc ttctgcgcgt cgtgcagcat ggaaatcact tgatagacgc gaccggcgac
     3001 gcgctcgtct tgctgaatca tcgcgcgcgc ggcgttcgcc gtaaggggta cgaactcgca
     3061 gaactcgggc tcgaacgggc acgcaatcgc gagcagcacc gtgagcgcca tcatgaacaa
     3121 gatcaccatc cgccagaaaa tgaggatggc catgacgagc gcgatgaaga tattgaccgc
     3181 gacgatcacg ttcatgccgc gcgcgttcca gagcgccgcc tcgtacgcgg tagcgtccgc
     3241 cgcatcttgc gcgttctctc gccagaggat gccaaagccg cagttggtga ggtgaaacag
     3301 gaccgcgacg aggaccgctc ccatcaggat gcccatcacc gcgatccccc cgcggcggtc
     3361 gtgcagcagc ttaccgaggt tcatcgcttc acctgagctc gcgcgacgcg tcgctcgagc
     3421 gcgcgcacga tcactgaata agcggggtcg gcgctgctcg cggcgagggc gcggtattca
     3481 gccagcgcat tcatccagtc gccggtcgcg tagtagtcgg cggcgcgctt cgagctcggc
     3541 ggcccgctca gcgtgggttc gaccgggcgc gacggcgccc gaaccgccgc gctcgctgcg
     3601 ggtgcgccct tcgcccgctg cggagtcgcc gcgcgagctt cggtctctgc cgcggcgggg
     3661 ttgagcatac caatacccga aatcacggcc gcgaccccga gcaacgagta cgcggcaagc
     3721 ttcagcttcg gctggggcgc ctgcgtcggt gtcggctcga gttccgcgaa ttctccctcg
     3781 aaagcttcga tgagctcctc atcgagggac ttcacctcca gccgccgcgc ctcggtcagc
     3841 tcctccggtt tgagttgcgg aggaggtgcc aaacgcagtt tggcagccgg cgcttgcgct
     3901 ttggcgggca cctcgggcaa ctcgagccca aggtccgaca aatcgaagac gcgagtctct
     3961 tggaagtggt tcatttcagg cgcagcctct ggcttgtgca gaggtcgtgc cggaatgtgg
     4021 gggtc
//
LOCUS       DASTKS010000175         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1588711, whole genome shotgun sequence.
ACCESSION   DASTKS010000175 DASTKS010000000
VERSION     DASTKS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588711"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VFQ35_03675"
     CDS             complement(<1..689)
                     /locus_tag="VFQ35_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929755.1"
                     /translation="MSASLPSPKPNSAEQKKLRDRAVQRELRDPLAPIQALGRRDLRI
                     GAILGIAGTLTVHGAPAADAITSFGDMRDFSERVVTVIHDRLKAEIDLDSEKPPPPPP
                     PPPPPEPEPPPQKEAPPPPRAAANEPPPPPPAPAQAGKVLTQEPDPDEPVDLTGEGFV
                     TGNADRFAGGVTHAAGTSTSAVRSTAATPSGVPGGRGTAPAPPPPPAEDRSRPATVDT
                     SVRWNCGFPPEA"
     gene            complement(694..1140)
                     /locus_tag="VFQ35_03680"
     CDS             complement(694..1140)
                     /locus_tag="VFQ35_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9929756.1"
                     /translation="MAGGASNDDDNGLIAGINVTPLVDVTLVLLIIFMVTAKIIVSQG
                     MPMDLPKAASGESLQTVFSVELTQDGKTRVDSQAVPSDEAIAQLAKDAHAKNKDLRAV
                     IRADRKVEHGRVIHVLDLLKRSGVAKIAFAVSPVAEPTPVEAPAGK"
     gene            complement(1144..1893)
                     /locus_tag="VFQ35_03685"
     CDS             complement(1144..1893)
                     /locus_tag="VFQ35_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9929757.1"
                     /translation="MNLVQWLQKLMTNFGAGWVMVLMIGLSVVSVAIMLERGWFYWSL
                     RDDITRLAQKLREELRRGDYDAARQALERSPSAEAAVVVAGLLEADRGPKAAEEAMEG
                     AIALQRMKLERRLAFLGTLGNNAPFIGLFGTVIGVVEAFEQLGKQSTVAVKAAAVAAP
                     QEVMAAIAEALVATAVGLAVAIPAVAMYNYYQRLAKATLANTSALSHVLLSYLVSPVE
                     QPPRRAEKSKLDAEPESRGAKSKPAHDEGEA"
     gene            complement(2242..>3573)
                     /locus_tag="VFQ35_03690"
     CDS             complement(2242..>3573)
                     /locus_tag="VFQ35_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HET9929758.1"
                     /translation="DMDYTLAVYRQDEMDRLSVELTVERLIRRGYPSYLNEIKYDPRF
                     PIRGLLVDKRYGHVLKMDRYKVVHCGYHGLKRIPREELQELYHDKRIRPHTPRYHWID
                     TLFALCEVTSYAAIVDALEQRGERIDFDKLFSDVRAAIDEAHADGTVYASLTADFPRY
                     VERDPELARTLHKLRSAGKHLFLLTNSPFHFTDQMMSYLLGGAMAEYPSWRHYFDIVM
                     CAAGKPRWFQEGRPMMEREGDVVKPVKAQLERGRIYEGGNLADFERLAGVQGSSVLYV
                     GDHIYGDILRSKKESSWHTAMIIPELDAEIEAHEVCTVDLSRQRALEEARDRLEDELR
                     YYQTRFKELSKAQPDTFLDAERARVKRALERIRGDLRANLSEHEILAERISRRFHPYF
                     GSLLKEQSEMSSFGLQVDLYADIYMRRVSCLKAYSPQQFFRSPYDLMPHEL"
BASE COUNT          568 a         1113 c         1232 g          660 t
ORIGIN      
        1 gcttcgggag gaaatccgca attccacctg acgctcgtgt cgaccgtcgc aggacgcgag
       61 cgatcttcgg cgggcggcgg gggtggcgcg ggagcggtgc cgcgacctcc aggtacgccc
      121 gacggcgtcg ccgcggtcga acggacggcg ctcgtcgaag tgccggcggc gtgagtgact
      181 cctcccgcaa aacgatcggc gtttccagtt acgaagcctt cgccggtgag atcgacgggc
      241 tcgtcggggt cgggttcttg cgtgagaacc ttcccggctt gggccggcgc agggggcggc
      301 ggaggaggtt cgttcgccgc ggcgcgaggt ggtggcggcg cttctttctg cggcggcggt
      361 tcgggctcgg gcggtggagg aggcggcggc ggaggagggg gcttttcgct gtcgagatcg
      421 atctcggcct tcagtcggtc gtggatgacg gtgacgactc gctccgagaa gtctcgcatg
      481 tcgccgaagg aggtgatcgc gtcggcggcg ggagcgccgt gcaccgtgag cgtgccggcg
      541 atgcccagaa tcgcgccgat gcggagatcg cgccgtccga gcgcttgaat cggcgcgagc
      601 gggtcccgta gctcgcgctg aacggcgcga tcgcgcagtt tcttctgctc ggcgctgttc
      661 ggcttcgggc tcgggaggga cgcagacatc aggttacttt cctgcgggcg cttcgaccgg
      721 cgtgggctcg gcgacgggcg agaccgcaaa ggcgatcttc gcgacgccgg agcgcttcaa
      781 caagtcgagc acgtggatga cgcgcccgtg ctccaccttg cggtcggcgc ggatgactgc
      841 ccgcaagtct ttgttcttcg cgtgcgcgtc tttcgcgagc tgcgcgatgg cttcgtcgct
      901 cggcaccgct tgcgaatcga cgcgcgtctt gccgtcttgc gtgagctcca ccgagaacac
      961 cgtttgcagc gactcaccgc tcgccgcttt cggtaggtcc atcggcatgc cctgcgaaac
     1021 gatgatcttc gcggtgacca tgaaaatgat gagcaacacc agcgtgacgt cgacgagcgg
     1081 cgtcacgttg atgccggcga tcaggccgtt gtcgtcgtcg ttggacgctc caccggccat
     1141 gtcttacgcc tcgccttcgt cgtgcgcggg cttcgatttc gccccgcgac tctcgggctc
     1201 ggcatcgagc ttgctctttt ccgcgcgacg cggcggctgc tcgacggggg acacgaggta
     1261 cgacaagagc acgtgcgaga gcgcagaagt gttcgcgagc gtggccttcg cgaggcgctg
     1321 atagtagttg tacatggcga ccgcggggat cgcgacggcg aggccgaccg cggttgcgac
     1381 gagcgcttcg gcgatcgcgg ccatgacctc ttgaggcgct gcgacggcgg cggccttgac
     1441 ggcgaccgtg ctctgcttgc cgagttgctc gaaggcctcg acgacgccga tgacggtgcc
     1501 gaacaaaccg atgaagggcg cgttgtttcc gagcgttccg aggaaggcga ggcggcgttc
     1561 gagcttcatg cgctgcagcg cgatcgcacc ctccatcgct tcttccgcgg ctttggggcc
     1621 gcgatccgcc tcgagcaagc cggcgacgac cacggcggcc tcggcgctcg gcgagcgctc
     1681 gagcgcttga cgagcggcgt cgtagtcgcc tcggcgcagc tcctcacgca gcttttgggc
     1741 gagccgagtg atgtcgtcgc gcagcgacca atagaaccag ccccgctcca gcatgatggc
     1801 gacggacact acgctcagac caatcatgag gaccatgacc caacccgcgc cgaagttggt
     1861 catgagcttc tgcagccact gcactaaatt catcggggcc ttccttgcct ttctacgagc
     1921 agtctcgaca tcgagccgtg gaggacattc cgcaacggcc gaggtgacgg ctttcttccc
     1981 gcggcacgag cccggcctca ccgccctctg ggcggcgttc ttcagaactc cgtgagaatg
     2041 actgggtgtt caccgggccc cgcgcggccg gtgaattagc acaaaagttt cgcggagcgc
     2101 tgaggagaaa caggtcggtg ggccggtttc ggcttctggc cgtgagacga cggaagagcc
     2161 gcaggtcagg gcagaatgac cgactgagcg ccccgatggg ccctcgtttc gggaccggag
     2221 cgcacccgcc ccccgatcaa ctcagagctc gtgcggcatg aggtcgtacg gagaccggaa
     2281 gaactgctgc ggcgaataag ccttcaggca ggagacgcgt cgcatgtaga tgtcggcgta
     2341 gaggtccacc tggaggccga agctcgacat ttcgctctgc tccttgagca gcgagccgaa
     2401 gtagggatgg aaccttcggg agatacgttc cgcgaggatc tcgtgctccg aaaggttcgc
     2461 tcgcaagtcg ccgcggattc gctcgagcgc acgtttgacc ctggctcgct cggcgtcgag
     2521 aaaggtatcc ggctgggcct tcgagagttc cttgaagcgc gtctggtaat aacgaagctc
     2581 gtcctcgagg cggtcgcgcg cctcttcgag cgctcgttgc cgcgacaggt ccaccgtgca
     2641 gacctcgtgc gcctcaatct cggcgtcgag ctctgggatg atcatcgccg tgtgccacga
     2701 cgactccttc ttcgagcgca agatgtcgcc gtagatgtgg tcgccgacgt agagcacgct
     2761 cgagccctgc acgccggcga gccgctcgaa atcggctagg ttgccgccct cgtagattcg
     2821 accgcgctcg agctgagcct tcaccggctt cacgacgtcg ccctcgcgct ccatcatcgg
     2881 ccggccctct tggaaccagc gcggcttgcc tgccgcgcac atgacgatgt cgaagtagtg
     2941 ccgccagctc gggtactcgg ccatcgctcc gccgagcaga taactcatca tctggtcggt
     3001 gaagtgaaac ggcgagttcg tcagcaagaa gaggtgcttg ccggccgaac gcagcttgtg
     3061 cagcgtgcgc gcgagctcgg gatcgcgctc cacgtagcgg gggaagtcgg cggtgagcga
     3121 ggcgtacacg gtgccgtccg cgtgcgcttc gtcgatcgcc gcgcgtacgt cgctgaagag
     3181 cttgtcgaag tcgatgcgct cgccgcgttg ctcgagcgcg tcgacgatgg ccgcgtagct
     3241 cgtcacttcg cacagggcga agagtgtgtc gatccaatgg tagcgcggcg tgtgcggccg
     3301 aatgcgcttg tcatggtaga gctcctgcag ctcttcacgg gggatgcgct tcaggccgtg
     3361 gtagccgcag tgcacgacct tgtagcgatc catcttgagg acgtgcccgt agcgcttgtc
     3421 gacgagcagg ccgcggattg gaaagcgcgg gtcgtatttg atctcgttca ggtagctcgg
     3481 gtaaccgcgc cggatgagcc gctcgaccgt gagctcgacg ctcaaacggt ccatctcgtc
     3541 ctgacgatac accgcgaggg tgtagtccat gtc
//
LOCUS       DASTKS010000176         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_696528, whole genome shotgun sequence.
ACCESSION   DASTKS010000176 DASTKS010000000
VERSION     DASTKS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696528"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..875
                     /locus_tag="VFQ35_03695"
     CDS             48..875
                     /locus_tag="VFQ35_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929759.1"
                     /translation="MAPSSPLSSRSRLLIYGAVATLSAASLALLKLAPFMLVGSFIAY
                     LALPRERGRALTVLTSAAALLSLAGFMRFLVVEAMPGIVQGGNRATEDSAVSRLRQVL
                     FAEDALRKRADVDPDHDGIGSAGLLSELAGYVGLRGGARLVPPLLERFPEPLDSPIGP
                     VMELSGYLFVVCLPAKNGGFTARPGEIVDEEAAERRFVAYAWPAADQRGLLNSFFMDE
                     HERILFAGNGAPAGKRPRIGLEHPPACDEALNPKTSEEWQTWKGKRPRQKLPGDKLP"
     gene            875..2077
                     /gene="dusB"
                     /locus_tag="VFQ35_03700"
     CDS             875..2077
                     /gene="dusB"
                     /locus_tag="VFQ35_03700"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9929760.1"
                     /translation="MFGTTVPTTPSYVSALNHSVPLAQPGEFKELHIGPLRVWPPVVL
                     APMAGVTDYPFRAICRRYGAGLYVSEMITARPLAEGNQKTEKLARFGPDENPRSLQLY
                     GVDPYFVGEAVKRLVGESWVDHIDMNFGCPVRKVTSRGGGAALPLKRRLLAAIVRAAV
                     SNAGRIPVTIKFRLGVNDELITFVETGRIAQEEGCAAVGLHARTAAQLYSGEARWDRI
                     AELKQALRIPVLGNGDIWEAHDALRMMRQTGCDGVIVGRGCLGRPWLFRELAEVFDGK
                     SPSVPPNLGEIADVMREHAERLAAWFGEKPALRSFRRHATWYTKGFRGSASLRERVMQ
                     METLDDLRRILEGIDRAEPFPVSAVRVQRGKSGGSQVVSLPQGYLDDLDDTSVPEDEV
                     GCEEMSGG"
     gene            complement(2346..2963)
                     /locus_tag="VFQ35_03705"
     CDS             complement(2346..2963)
                     /locus_tag="VFQ35_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929761.1"
                     /translation="MGAEDGLGAAGIAGAGGAGEAFGGPAAGGAPFAGAPLGGGALGG
                     GALGEAPGGAAGFGGAPLGTATGTGTAMAAFAGAPCGKTCVAGMGGGAAAAGAGAGGS
                     ATGMGGGGGGGGGGGGSGRSSGGLKPRRGKWAASRAGAGGALRTAPPLGGSVALSLSV
                     TLGEMDAANEVARWGLMLARRLLGPDEIGAPLPIWSGIATTAKST"
     gene            complement(3081..3458)
                     /locus_tag="VFQ35_03710"
     CDS             complement(3081..3458)
                     /locus_tag="VFQ35_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET9929762.1"
                     /translation="MSSSESEQIRIDRWLCAARIYKSRTQAHDACEGGLVKINDESAR
                     ASQAVRVGDEVRAHAPRGLVILKVLKLAPKRLGPQPARELYEDHSPPPPPREERIAVR
                     ERGAGRPTKADRRAMERLRTRDD"
BASE COUNT          575 a         1243 c         1177 g          587 t
ORIGIN      
        1 gaccagggcg tgccgtttcg cccaacggcc gctaagctcg cgccgccgtg gcaccgtctt
       61 cacccctgtc atcacgctcc cgtctcttga tttacggggc cgtcgcgacg ctttcggcgg
      121 cgtcgctcgc gctgctcaag ctcgccccgt tcatgttggt gggctcgttc atcgcctacc
      181 tcgcgctacc gcgggagcgg ggccgggcgt tgaccgtgct cacctcggcc gccgcgctgc
      241 tctcgctcgc gggcttcatg cgttttctgg tggtggaagc gatgccgggc atcgtgcagg
      301 gcggcaatcg cgcgaccgaa gactcggcgg tctcgcgcct ccgacaagta ctcttcgcgg
      361 aggacgcctt gcgcaaacga gccgacgtcg acccagacca cgacgggatt ggttcggcgg
      421 ggctcctttc cgagctcgcc ggctacgtgg gtttgcgcgg tggggctcgg ctcgtgccgc
      481 ccctgctgga gcgctttcca gaaccgctcg actcgccgat cgggcccgtg atggagcttt
      541 cggggtacct tttcgtcgtg tgtttgccgg ctaaaaacgg ggggttcacc gctcgcccgg
      601 gcgagatcgt cgacgaagag gctgcggagc gccgcttcgt cgcgtatgcc tggcccgccg
      661 ccgaccaacg cgggctgttg aactcctttt tcatggatga gcacgaacgc atccttttcg
      721 caggcaacgg cgctcccgcg ggcaagaggc cgcgcatcgg gctcgaacac ccccccgcct
      781 gcgacgaagc gctgaatccg aagacgagcg aagaatggca aacctggaag ggcaagcgcc
      841 cgcgacagaa actgccggga gacaagctcc cgtgatgttc ggtaccaccg tcccgacgac
      901 gccgagctac gtcagcgcac tgaaccattc ggtcccgctc gcgcaaccgg gagagttcaa
      961 agagctgcac atcggaccgt tgcgtgtttg gcctcccgtc gtcttggcgc cgatggctgg
     1021 agtcaccgac tacccgttcc gagccatctg ccggcgctac ggcgcggggc tctacgtgag
     1081 cgaaatgatc acggcgcgac ctctcgccga gggcaaccaa aagaccgaga aactcgcgcg
     1141 cttcggcccc gacgaaaacc cgcggagctt gcagctctat ggggtcgatc cctacttcgt
     1201 cggtgaagcg gtcaagcggc tcgtcggcga aagctgggtc gatcacatcg acatgaactt
     1261 cggatgcccg gtgcgcaaag tgacttcgcg cgggggcggc gcagcgctcc ctctcaagcg
     1321 gcgtttgctc gcggcgatcg tgcgcgcggc ggtcagcaac gcgggccgca ttccggtcac
     1381 catcaagttc cgactcggcg tgaacgacga gctcatcacg ttcgtggaga cgggccgcat
     1441 cgcgcaagaa gagggctgcg cggcggttgg gcttcacgcg cgcacggccg cgcagctcta
     1501 ctcgggcgag gcacgctggg atcgcatcgc cgagctcaag caggcgttgc gcatcccggt
     1561 gctgggcaac ggcgacattt gggaagcgca cgacgcgctc cgcatgatgc ggcagaccgg
     1621 ctgcgacggc gtgatcgtgg ggcgcggctg cctcgggcgg ccctggctgt ttcgcgaact
     1681 cgcggaggtg ttcgacggca agtccccgtc ggtgccaccg aacttgggag agatcgccga
     1741 cgtcatgcgc gagcacgccg aacggctcgc ggcgtggttc ggagagaaac cggcactccg
     1801 atcctttcgt cgtcacgcga cttggtacac gaagggattc aggggcagcg cgagtctccg
     1861 tgagcgcgtc atgcagatgg aaacgctcga cgatttgcgg cgaattttgg aagggatcga
     1921 ccgagcggag ccgttcccgg tctcggcggt ccgcgtgcaa cgcggcaaga gcggcggcag
     1981 ccaggtcgtg agtttgcccc agggctatct cgatgatttg gacgatacga gcgtgcccga
     2041 agacgaggtc ggctgcgagg agatgtccgg tgggtaagcc tccccagggg cgcccatcac
     2101 cgagccgcac gagggccgag gcgcgatgga aaatcggggc gaaatagccg cgaaatccga
     2161 cgctcatgcg cccgtcatat aaaaaaatcg cgctccgaga cgcatgagac acgagactcc
     2221 ccgatcgtct cggtgtaaag tgaccatacc tcggggacgc tgatgcgcgc gctcatcaga
     2281 ctgtggacag agattgcatc gtggcgctgg tcgccggcga ttgcattggc tttgggtgcc
     2341 ctggtttacg tgctcttcgc ggtcgtcgcg attccggacc aaataggcaa cggcgcgccg
     2401 atctcgtcgg ggccgagcag tcgcctagcg agcatcaggc cccagcgcgc cacctcgttc
     2461 gcggcgtcca tttcgcccag cgtgacggat agcgaaagtg cgaccgagcc gccgagcggc
     2521 ggcgcagtgc gcagcgcgcc accggcgccg gccctcgacg cggcccattt tccgcggcga
     2581 ggtttcagcc cgccactcga gcgcccggag cctccgcctc cgccgcctcc tccccctcca
     2641 cctcccatgc cggtggcgct ccctccggcc ccggctcccg cggccgccgc gccgccaccc
     2701 atgccggcca cgcaagtctt gccacagggt gcgccggcga acgcagccat cgccgtgccc
     2761 gtcccggtcg cggtgcctaa cggtgcgccc ccaaatcctg cagcccctcc gggcgcttcg
     2821 cccaacgcgc cgccgcccaa cgcgccgccg cccaacggcg ctcccgcgaa tggcgccccg
     2881 cccgctgcgg gccccccgaa cgcctcgcca gccccgccgg caccggcgat gccagcggcg
     2941 cccaacccgt cttcagcacc caacgtgacg ccgccgcaat cgggcccacc aggagcagcc
     3001 ccgactcccc ccgctcccta accgcggcga gccagagcgt tccgcgcagg ggtgttcgta
     3061 gaaagacgta gacgccgaag ttagtcgtct cgggtgcgta aacgctccat cgcgcgacgg
     3121 tctgccttcg tggggcgccc ggccccgcgc tcgcggacgg cgatgcgctc ctcgcgggga
     3181 ggcggcggcg gcgaatggtc ttcgtaaagc tcgcgcgcgg gctgcggtcc caagcgcttc
     3241 ggcgcaagct tcaacacttt caaaatcacg aggccgcgcg gcgcgtgtgc tcgcacttcg
     3301 tcacccacgc gaacggcttg gctcgcgcga gccgactcgt cgttgatttt gacgaggccg
     3361 ccctcacaag cgtcgtgggc ctgcgtacgt gatttgtaaa tgcgcgcggc gcaaagccag
     3421 cgatcgatcc ggatttgttc gctttcggac gaactcacct tcttagcgtg cctgaaatcg
     3481 gcgcaacgtc cacgcgcctc gcgattggcc cgaggagttc gtcgcgtcgc cacacggccg
     3541 ctaagattcc aagcttgatt cgaggttcgt gagaacccgg ag
//
LOCUS       DASTKS010000177         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_28063,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000177 DASTKS010000000
VERSION     DASTKS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28063"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFQ35_03715"
     CDS             <1..570
                     /locus_tag="VFQ35_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929763.1"
                     /translation="QLTPAGCDLLNACTGDPAVRYGSVVARAERATFRRLLRSAWDLY
                     SQIVYPIYWAFRFIVARGDSSMVPAPVAEQRERLLEHYGALPSERDNDGIVPTNSQVW
                     GELIYATRADHLDVVGQFGRVDPDSWSGDWIPSYSGFDKQEFNALWSAICDFILRAPA
                     ALAAKPGVGSERTERDMLSESSATRPSLP"
     gene            681..1478
                     /locus_tag="VFQ35_03720"
     CDS             681..1478
                     /locus_tag="VFQ35_03720"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9929764.1"
                     /translation="MLPKLSPHQAETVRRTQEHFAREQGVTALLLGGSLAHGFATPAS
                     DVDVMIVVSDDEHSDRLSTARSCFFSKELCTYESGYVDGKYISEGFIRKVNERGSEPA
                     RFAFKDAVVLFSKSETLPPLLEEVSRYPVAEKAERIRRFRAQFEAWFWYSGEALKKDD
                     WPLFRTSIAKLSLFAGRAILARNELLYPYHKWFLRVLATATDKPHGFDERLVALAHSP
                     TRADVEEFASMTRDFMGWEFEHVTWPPQFMLDSELNWLRQEPPVDDI"
     gene            complement(1661..2167)
                     /locus_tag="VFQ35_03725"
     CDS             complement(1661..2167)
                     /locus_tag="VFQ35_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929765.1"
                     /translation="MNLAPPSSSSAVPPAIEHFLLERVATHEQLQSLLALFNIADAAV
                     TAVELEATVGLPEGEGQEALEALVDAQLVMRSGENPPRYRYSPARSSLDALVRLLALI
                     YSQHRYDVVRVMGENAIKRMWSAARLAFLVALSSESRSLARSPYADSRRRPKSRGARN
                     ADTRGDNV"
     gene            complement(2319..2738)
                     /locus_tag="VFQ35_03730"
     CDS             complement(2319..2738)
                     /locus_tag="VFQ35_03730"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HET9929766.1"
                     /translation="MQTGWSCPRCRRKFTRLNQRHACGTGDTREVLRDRPESLVRLYE
                     SLETFVKALGEVEVVARERYVLFRSVRIFADLVVTTDALRIAVHLPDAVDDAIFFKVV
                     RGERAVAHVAKLRSSKDLSVVESYVRSAYRFSLSKRE"
     gene            3122..>3657
                     /locus_tag="VFQ35_03735"
     CDS             3122..>3657
                     /locus_tag="VFQ35_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9929767.1"
                     /translation="MDRSDVSGEQPAPKIAMDLPIKEHILMVNDLPRGQAYLDAMREK
                     IRPGDVVLEVGTGAGLLSCMAANLGAKRVYTVEQSPELQRVATRVFEANGVADRVTLI
                     NAHSRDLVPKSLVPEPVDVFVTETIGTQGLDEAILQIFEHVKPLLAPGARTIPENVVF
                     KHCLVNMVGIRERFEVMHP"
BASE COUNT          709 a         1182 c         1143 g          623 t
ORIGIN      
        1 cagctgacgc ccgcgggctg tgatttgctc aatgcgtgca ccggcgaccc ggccgtacgc
       61 tacggctcgg tcgtggcgcg cgccgagcgg gcgacgttcc gtcggttgct gcgcagcgcc
      121 tgggatctct actcgcaaat cgtttacccc atctattggg cttttcggtt catcgtggcg
      181 cgcggggaca gctcgatggt gccggctccg gtcgccgaac aacgagagcg gctactcgag
      241 cactacggcg cactgccgag cgaacgcgac aacgacggca tcgttccgac caactcccaa
      301 gtttggggcg agctcatcta tgcgacgcgc gccgaccacc tggacgtcgt gggccaattc
      361 ggccgcgtcg atcccgacag ctggtccggc gactggatcc cgagctactc cggcttcgat
      421 aagcaggagt tcaacgcgct ttggagcgcg atctgcgact tcattctgcg tgcccccgcc
      481 gcgctcgccg ccaaaccagg cgtcggctcg gagcgaaccg agcgcgacat gctgagcgag
      541 agcagcgcca cgaggccgag ccttccctga gcggcttcgc aaacggaacc ggtagccggg
      601 cttcgcttcc gagcttcacg aggccgcgat gattgcgagc gcaagcgcgc ccctcggtat
      661 taaactgctc gccctccccg atgctcccca agttatcgcc acatcaagcc gagacggtac
      721 gcagaaccca ggagcacttc gctcgagagc agggcgtcac ggcgctcctg ctcgggggct
      781 cgctcgcgca cggcttcgcg acacccgcgt ccgacgtaga cgtcatgatc gtcgtatccg
      841 acgacgaaca cagcgaccgc ttgagcaccg cgcggagctg cttcttcagc aaagagcttt
      901 gcacttacga gagcggctac gtcgacggga aatacatctc cgaaggattc atccgcaagg
      961 tgaacgaacg cggcagtgaa ccggcacgct tcgccttcaa agacgcggtc gtgctcttct
     1021 cgaagagcga gacgctgccg ccgctcctcg aggaagtatc gcgctaccca gtcgccgaga
     1081 aggcggagcg catccgccgc ttccgcgccc aattcgaggc gtggttttgg tacagcggcg
     1141 aagcgctgaa gaaagacgac tggccgctct tccgtacctc catcgcgaaa ctctcgctct
     1201 tcgctggccg cgccatcctc gctcgcaacg agctcttgta tccgtaccac aagtggtttc
     1261 tcagagttct cgcgaccgcg accgacaagc cgcacggctt cgatgagcgt ctcgtggcgc
     1321 tcgctcattc accgactcga gccgacgtgg aagagttcgc ctcgatgacg cgcgacttca
     1381 tgggatggga gttcgagcac gtcacgtggc cgccccaatt catgctcgac agtgagctca
     1441 actggttgcg ccaagaaccg cccgtcgacg atatctgaac acctcgctgc gcggggttcg
     1501 cttgcgctga ctggtaggtg aacccagcga tcacactggg cagctgtccg gtgtttccgc
     1561 gaatttactt ctcgatccgc gaagcgcgcc gagcgtgcgg agcgcgtcgt tcacggagag
     1621 ggctcgagcg tactaccgtg agacgctgaa gccgcaactg tcacacattg tcgccgcgcg
     1681 tgtcggcatt gcgcgccccg cgtgacttgg gccggcgccg cgagtccgcg tacggcgagc
     1741 gcgccaagct acgcgactcg gaagagagcg cgaccaggaa cgccaaacgc gcggcgctcc
     1801 acatccgctt gatcgcgttc tcgcccatga cccgcacgac gtcgtatcga tgctgcgagt
     1861 agatgagagc caagagccgg accaaagcat ccagcgacga gcgggcgggc gaatagcggt
     1921 agcgcggggg gttttcgccc gagcgcatga cgagctgggc gtcgacgagt gcctcgagcg
     1981 cttcctgacc ctcaccttcg ggcaggccca ccgtcgcttc caactcgacc gccgtaaccg
     2041 ctgcgtcagc gatattgaac agcgcgagca agctctggag ctgctcatgc gtcgccactc
     2101 gctcgagcaa gaagtgctcg atggcgggcg gcacggcgct gctggaggaa ggtggagcga
     2161 gattcatggc tcagcgaagg ttggtcacgg aattcatgtg tacaagaagc atgccgggat
     2221 gctgaaaagc gctgcgggta caatccgagc caatggacct tcgccatgtc cggtggacac
     2281 tggagatctt cgccagtcag agacgtacgg tggcctcgct attcgcgctt cgaaagcgag
     2341 aaacggtacg cggaccgtac gtacgattcg acgacgctca ggtcctttga cgaccgcagc
     2401 ttcgcgacgt gcgcaaccgc gcgctcgccg cgcaccacct tgaagaatat ggcgtcgtcg
     2461 accgcgtcgg gcaaatgaac cgcgattcga agcgcgtcgg tggtcacgac gagatccgcg
     2521 aagatgcgca cgctgcgaaa caggacgtaa cgttcgcgcg cgacgacctc aacctccccg
     2581 agcgccttca cgaaggtctc cagagactcg tacaggcgaa cgagtgactc gggcctatcg
     2641 cgtagaacct cacgagtgtc tccggtgccg caagcgtgtc gctggttcag tcgcgtgaac
     2701 ttgcgacgac aacgagggca gctccaaccg gtttgcatga tacggattcg agagtgatgc
     2761 cgactcgagg gtgggtgttc gatgacgagc ctaccctcgg gagtaacgtc gctgccctgc
     2821 cccggggctc acgattgatg agtccccggg gcctcccgtt cgaactcgag acgggcgggc
     2881 cggaggctca gggattagcg ctgccagcgc cgcgatcgca cgtcaatcgc aatctgcgac
     2941 tgcctggtgc cgacggtgag cggtgactca atacgactgc agtgaggacc gacatcgccc
     3001 gaacgaaggc tcgaacccct ccgcgtgacg gtcgattaga ctgcgcttga ccgaagcgga
     3061 gccttgccgc agtgtgatga ccaagaaacc cgaggccggc ccgacctcga aagaagcgtg
     3121 gatggatcga agcgacgttt ccggcgaaca acccgcgccg aagatcgcga tggacctccc
     3181 catcaaggaa cacatcttga tggtgaacga cctgccgcgt ggacaagcct acctggacgc
     3241 catgcgagaa aaaatccgcc ctggcgacgt cgtgctggag gtgggaaccg gcgcaggtct
     3301 cttgagctgc atggccgcga acctcggcgc caagcgcgtt tatacggtcg agcagtcgcc
     3361 cgagcttcag cgagtcgcga cgcgcgtgtt cgaggcaaac ggcgtcgccg accgagtaac
     3421 gctcatcaac gcgcactccc gcgacctcgt gccgaagagc ctcgtgccgg agccggtcga
     3481 cgtgttcgtc accgagacca tcgggacgca gggcctcgac gaggcaattt tacaaatttt
     3541 cgagcacgtt aagcctttgc tcgcgcccgg agcccgcacc atccccgaaa acgtggtgtt
     3601 caagcactgc ctcgtgaaca tggtcggtat ccgcgagcgt ttcgaggtga tgcaccc
//
LOCUS       DASTKS010000178         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_474336, whole genome shotgun sequence.
ACCESSION   DASTKS010000178 DASTKS010000000
VERSION     DASTKS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474336"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFQ35_03740"
     CDS             complement(<1..789)
                     /locus_tag="VFQ35_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929768.1"
                     /translation="MTRATSVHRAARLAKLPAVPPATVAEKGSLPPAGAANAGQLVFE
                     AAAPDARWVTVCQARNDTNGDGRVSTTVSERGEWLGDRMQRYLELATGAEQAIEDLLL
                     SSPDGRWLVLRLDGHVELYDTLTGARRDLSALGADSRPEPNDFTNHRALAFSTDSLFY
                     LRNGDGGSELVRLSLLDGNELVLLKSSDPIARLRLDPAGKLITLSIARAENARNGRFV
                     WPHRLDDAPRPCRGPFARFQGPNPNADPLTSAVVDVDGAHAEFVD"
     gene            939..1421
                     /locus_tag="VFQ35_03745"
     CDS             939..1421
                     /locus_tag="VFQ35_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929769.1"
                     /translation="MNRQLVRRFLTRRAIAFAGAFAALGCGDKGAVTLTAQILNPTLS
                     TQSVALGTSLNGSFELRLDLGPEASNSTTVTPGAFAVRNASGEVTKVSVSFDSPPPYE
                     LSKGGSKSVTGTLDATKLVDAQLRDALCAGQVWYAATLTDTLSGNTTAQSAAFSPTCN
                     "
     gene            1435..3024
                     /locus_tag="VFQ35_03750"
     CDS             1435..3024
                     /locus_tag="VFQ35_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9929770.1"
                     /translation="MTKPKHFETWSGVEVPAVARPEDAGGDPGRDLGEPGAYPFTRGP
                     HASMYREKPWTMRMFAGFGTPADTNQRFRFLLAEGQTGLSTAFDFPTLMGYDSDSPRA
                     LGEVGVCGVAVDTLRDFEVLFDGIPLDRVTTSMTINGPALVLLAFYVALADLRGVDRR
                     ALGGTIQNDCLKEFIAQHAWLVPPRPAMRIVSDMIEFCSREMPRYNSVSISGYHIREA
                     GSTAAQELAFTLANGLAYVEECRARGLDVDDFAPRLSFFFDVHNDFFEEIAKFRAARR
                     MWARFMKERFGAKREQSLKLRTHAQTAGVSLTAQQPYNNVVRVALQALAAVLGGTQSL
                     HTNSLDETYALPTEEAVMLALRTQQIILHESGVANSVDPLAGSYHVEWLTNRLEADAL
                     AYIERIDQMGGMVRAVESGYPQREIARSAYEFEQKLGRGERVVVGVNAFQRAEGETIP
                     TLRVDESVGRNKALELAKLKAERDQAAVQAALVKVRRAAETSENLVPPVVEAARAYAS
                     LQEICDVLRSVFGTYTDPAVF"
     gene            complement(2949..4703)
                     /locus_tag="VFQ35_03755"
     CDS             complement(2949..4703)
                     /locus_tag="VFQ35_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9929771.1"
                     /translation="MKTRSITHRRAEALRFTLLTALGALPLNACGGNTSESGGAGGRA
                     GSGGTAANGGGYAKGGGFSNGGGYTEGGAQASGGATGLGGALATNGGASPTGGAQPFG
                     GANTTGGAQPFGGASTTGGASGGANTTGGASSKFPCENPMPVPGDTTGTFEVCAGGWK
                     HRTAVRECPSSLPRSRVLPPSRVPNVDACTSDADCTAQANGHCEPPTLVGADLPVNSC
                     WYGCVRDADCGASGAICECGDPVGTCVAARCVSDADCGGAGLCATYDSEPGCDIIAYA
                     CQTPMDVCAADADCPNLQECSIKAGSSARVCQSQNCAIGRPFLVGGAIRCAAAIERAD
                     WAERSADSDDVLRPEAREALAHYWARIGSLEHASIAAFARFNLELLSFGAPAELIELT
                     NRALADETLHAKLAFSLASRYQGALLGPGPLDMRGAVIHATLLESVLAAFEEGCIGET
                     VAALEAARALEGSTDEAVRAALTRIADDEARHAELAYRYLRWALPQLGPEIARELVRR
                     VERECENAAQPGRSVTHDPTLSRHGLLSPEESQGVRRAALVEVVLPCVRALAQNTAGS
                     VYVPNTERSTSQISCRLA"
BASE COUNT          776 a         1656 c         1642 g          783 t
ORIGIN      
        1 gtcgacgaac tcggcgtggg ccccgtcgac gtccacgacc gcgctcgtca gcgggtcggc
       61 attgggattc ggcccctgaa agcgcgcgaa gggcccgcga caggggcgcg gcgcgtcgtc
      121 gagccgatga ggccaaacga agcgaccatt ccgcgcgttc tcggcgcgcg caatgctcag
      181 cgtgatgagc ttgcccgcgg ggtcgagccg cagccgcgcg atgggatccg agctctttag
      241 cagcacgagc tcgttcccgt cgaggagcga gaggcgcacg agctcgctgc cgccgtcgcc
      301 gttgcgcagg taaaagagcg agtccgtcga aaacgcgagg gcgcgatggt tggtgaaatc
      361 gttcggctcc gggcgactat cggcgccgag cgcgctgaga tcgcggcgcg caccggtgag
      421 tgtgtcgtag agctcgacgt ggccatcgag gcgcaacacg agccagcggc cgtccggcga
      481 gctcagcaag agatcttcga tcgcttgctc tgctccggtc gcgagctcca ggtaccgttg
      541 catgcggtcg ccgagccact cgccgcgctc cgacactgtc gtgctgacgc gaccatcgcc
      601 gttggtgtca ttgcgcgcct ggcacacggt gacccaacgc gcgtcgggag cagcggcctc
      661 gaacaccaac tgccccgcgt tcgcggcccc cgcggggggc aacgagccct tctccgccac
      721 cgtcgcggga ggaactgcgg ggagcttggc cagccgagca gcgcggtgca ccgaggtcgc
      781 tcgtgtcacc gcgctacctg gcgccgtgat aggagcgggc ggtttcgagc acgcgagcac
      841 gatcgaggcg atggcgggaa gtacggagac gcgcaacgcg gggaccatag ccgagttccc
      901 cgccgcggag cgacaaagcg atgctacgct ctctcgccat gaatcgtcag ctcgtgcgcc
      961 gtttcttgac tcgccgcgca atcgctttcg ccggtgcttt cgccgcgctc ggctgcggtg
     1021 acaagggtgc tgtcaccctc acggctcaaa ttttgaaccc caccttgagc acacaaagcg
     1081 tagcgctcgg aacgagcctg aacgggtcgt tcgaactccg actcgatctc ggccccgagg
     1141 cgtcgaactc gacgacggtg acgcccggcg cgttcgcggt tcgcaacgcg agcggcgagg
     1201 tcaccaaggt gagcgtctcg ttcgactcgc ccccaccgta cgagctatcg aagggcgggt
     1261 cgaagagcgt caccggcacc ctcgatgcga ccaagctcgt agacgcgcaa ttgcgcgacg
     1321 ccttgtgcgc gggccaggtt tggtacgcgg cgacgctgac cgacaccttg agcggcaaca
     1381 ccacggcgca gagcgccgcg ttctcgccga cgtgcaactg aggcgaagca gccggtgacg
     1441 aagcccaaac acttcgaaac ctggagcggc gtcgaggtgc ccgccgtggc gcgccccgaa
     1501 gacgcgggcg gcgatcccgg gcgcgacctc ggcgagcccg gcgcgtaccc cttcacccgc
     1561 gggccgcacg ccagcatgta ccgggaaaag ccctggacca tgcgcatgtt cgcggggttc
     1621 gggacgccgg cggacacgaa ccagcgcttt cgcttcttgt tggcagaagg ccagacgggg
     1681 ctctctaccg ccttcgattt cccaaccctg atgggttacg actctgactc gccccgcgcg
     1741 ctcggcgagg tcggggtgtg cggtgtcgcg gtcgatacat tgcgagactt cgaggtgcta
     1801 ttcgatggca tcccgctcga tcgcgtcacg acgtcgatga ccatcaacgg tcccgcgctc
     1861 gtgctgctcg ctttttacgt cgcgctcgcc gatctacgtg gcgtcgatcg gcgcgccctc
     1921 ggcggcacga ttcagaacga ttgcctcaag gagttcatcg cgcagcacgc ctggctcgtg
     1981 ccgccgcgcc cggccatgcg catcgtgagc gacatgatcg agttctgctc gcgcgagatg
     2041 ccgcgctaca acagcgtttc gatcagcggc taccacatcc gcgaagccgg ttcgacggcg
     2101 gctcaagagc tcgcgttcac cctcgcgaac ggccttgcct atgtcgaaga gtgtcgcgcg
     2161 cgcgggctcg acgtcgacga cttcgcgccg cggctcagct tcttcttcga cgttcacaac
     2221 gatttcttcg aggagatcgc gaagtttcgc gcggcgcggc gcatgtgggc gcgcttcatg
     2281 aaggagcgct tcggcgcgaa gcgcgagcag agcctcaagc tccgaaccca cgcgcagacg
     2341 gcgggcgtga gcctcaccgc gcagcagccg tacaacaacg tcgtgcgggt ggccctgcag
     2401 gcgctcgcgg ccgtgctcgg cggaacgcag tcgctccata cgaactcact cgacgagacc
     2461 tacgccctac ccactgaaga ggcggtgatg ctcgccttgc gcacgcagca gatcatcttg
     2521 cacgagtcgg gcgtcgcgaa cagcgtcgac cccctcgcgg gttcgtatca cgtcgagtgg
     2581 ctcaccaatc ggctcgaggc cgacgcgctc gcgtacatcg aacgcatcga tcagatgggg
     2641 gggatggtgc gcgcggtcga gtccggctac ccgcagcggg agatcgcgcg cagcgcttac
     2701 gagttcgagc agaagctcgg tcgcggcgag cgtgtcgtcg tcggcgtcaa tgccttccaa
     2761 cgcgccgaag gcgagaccat cccgacgctg cgcgtggacg agagcgtcgg ccggaacaag
     2821 gcgctcgagc tcgccaagtt gaaagcggag cgcgatcagg ccgcggtgca agcggccctg
     2881 gtgaaggtgc gccgtgccgc tgagacctcg gagaacctcg tgcctccggt cgtcgaagcg
     2941 gcgcgagcct acgcgagcct gcaggagatc tgcgacgtgc tgcgctcggt gttcggcacg
     3001 tacacggatc cggccgtgtt ctgagccaag gcgcgcacgc acgggagtac gacttcgacg
     3061 agcgccgcgc gtcgaacgcc ctggctctcc tcgggtgaga gcaagccgtg ccggctgagg
     3121 gtcgggtcgt gcgttaccga gcgtcccggt tgcgcagcgt tttcgcactc gcgctccaca
     3181 cggcggacca gctctcgagc gatttccggg cccagttgcg gcaaagccca gcgcaagtag
     3241 cggtaggcga gctcggcgtg acgggcttcg tcgtcagcga tgcgggtcag cgccgcacga
     3301 acggcctcgt cggtcgaccc ttcgagcgcc cgcgccgcct ccagcgccgc gacggtctcg
     3361 ccgatgcatc cctcctcgaa cgccgcgagc acgctctcga gcaaagttgc gtggatgacg
     3421 gcgccgcgca tatcgagcgg gccagggccg agcaacgcgc cctgatatcg gctcgccaac
     3481 gagaacgcga gcttcgcgtg caacgtctcg tcggcgagag cccggttcgt gagctcgatg
     3541 agctctgccg gcgctccgaa cgagagcagc tcgaggttga agcgcgcgaa cgcggcgatc
     3601 gaggcatgct cgaggctgcc gatgcgcgcc caatagtggg cgagggcttc ccgcgcttct
     3661 ggccgaagca cgtcgtccga gtcagcggat cgctccgccc agtccgcgcg ctcgatcgcg
     3721 gcggcgcacc ggatggctcc gcccaccaga aatggtctcc cgatcgcgca gttctggctc
     3781 tggcaaacgc gcgccgaaga gcccgctttg atgctgcact cctggaggtt cgggcaatcg
     3841 gcgtcggcgg cgcaaacgtc catgggcgtt tggcacgcat aggcgatgat gtcgcagccc
     3901 ggctccgagt cgtaggtcgc gcaaagcccg gcgcctccgc agtccgcgtc gctcacgcag
     3961 cgcgcggcta cgcacgttcc gacggggtcg ccgcactcgc agatcgcccc cgatgcgccg
     4021 caatctgcat cgcgcacaca accgtaccag cacgagttta cgggcaggtc ggcgccgacc
     4081 agcgtgggcg gttcgcagtg accgttcgct tgcgcggtgc agtcggcgtc gctcgtgcag
     4141 gcatcgacgt tcggcacccg tgaggggggg agcaccctgc tacgcggcaa gctcgaaggg
     4201 cattcgcgca cggccgtgcg gtgcttccaa cctccggcac agacctcgaa ggtgccggtg
     4261 gtgtcgcccg gcacgggcat cggattttca catgggaact tggagcttgc gccaccggtg
     4321 gtgttcgcgc ccccacttgc gccgccggtc gtactcgctc cgccgaaggg ttgcgcaccg
     4381 ccggttgtgt tcgctccgcc gaagggttgc gcgcctccgg tagggctcgc gccgccattc
     4441 gtcgcaaggg ctcctccgag acccgtggcg ccgccgctcg cctgagctcc gccctcggtg
     4501 tatccgccac cgttcgagaa gcctccgccc ttggcgtatc cgccaccgtt ggctgcggtc
     4561 ccaccgctgc ccgctctgcc gccggcgcct ccgctctcgg aggtgttccc tccgcaagca
     4621 ttcaatggca aggcgccgag cgcggtgagg agcgtgaagc ggagtgcctc ggctcggcga
     4681 tgggtgatcg atcgcgtttt caaattgttg ctcctcgaag ttcgcagacg ccgtgctcga
     4741 ggcttggggt ccaaccgatt gggcgctccc gatcgtcgag caacgcgcgc acacagggca
     4801 aaattacgtc tttcaatgcg gcctgacgca ccgctcggcg cgcgttcgtc gagagta
//
LOCUS       DASTKS010000179         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1366324, whole genome shotgun sequence.
ACCESSION   DASTKS010000179 DASTKS010000000
VERSION     DASTKS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366324"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VFQ35_03760"
     CDS             <1..1605
                     /locus_tag="VFQ35_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET9929772.1"
                     /translation="DALRSQLRAALRESTTRVVMVSVRTVAEKARRIVLSLTSDYLRN
                     PSRPAPVLLRLMPQPVEFRDLVATAFDARRVPFSRFDFPGLLAEGAFLPVFTVDALRS
                     ALNPSSGAKAALQVLARGGKVVLVTSEEVKPEELARHWGMRASNIRTLPRTAPPTGRG
                     KGPLPPPSGGQPVLPPPPGDRFDRGRALLVGVANYPRVSQLPESVLNDARDIAALLRS
                     PIRCGYPESNVEVLLDQQATAQRFRFALQRLAQTTSPDDTVVIYFSGHGAREINQGRV
                     EAYLLPFDSDPDDLERTALSASELSRLLAAIRASRLVVLLDACHAAGAAHLKTAKSAT
                     FKSGLDDKTYEALGRGTGRVVMASSRADEPSRLLPGMKNSLFTAYLLEALDGTAKNHG
                     DDVIRVLDVFHHIAENVPRRAPQHPILKAQDVENNFAIALNLRTKQASPLPSAPSHEV
                     PYRVASPLPPKARLAVSRGLVRRWQELAVYLGVPLQDKVTFDRGRESHGVLEWLEERG
                     RLGQLRDAFIDLGWTDLVDELDRHSS"
     gene            1665..>5090
                     /locus_tag="VFQ35_03765"
     CDS             1665..>5090
                     /locus_tag="VFQ35_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9929773.1"
                     /translation="MTQQMWFCRGGERVREAFDAALAWCEGCCVVTESPTAADASDEL
                     WLQLGRASDRVRAAILANIEAAESDLIAPFFNAGRIRRLVDATHHDHFIWFERDGEVR
                     FLLAPAALRKSTMDGSGLWMYCEGQRGALPEGLQTAIESVWSASVLSEPAEVLGGAAS
                     VGAPAAYSRPIPSVREVLGIRTPRDYFELEEADQERALWSTLIGEGPVDLEQGIRLCA
                     ERLRAQGFLDYQRLHQDGRVYSALEERLLAARRRLNLFDRPRSGFVRAIQSEIGSVTA
                     EQWRDCLMGALEQGAHVDRDEAVRLGFEYAQATYGVNVQRLRNGGRADQALRSAINSS
                     VRQGYLRRDGAAYLLRVALQTEPTLRGAIGVGADAPNPAQETASSPLGDGIEAAPVAM
                     NVETDASTPAASEATPSSPNTEAESLPLDRDVQSLPLPARTLNWCARCEVSTLRELVA
                     WQPAAFENEKNVGPSTVRETREVIESLLGCTWEQAHSSVHDRAVAIREQAQDTDTSED
                     DAPADSVTAGGAAAWVRLAAQLSDEARMTPLVDIQLPTRMRNYAQSEGLERLGDLFSL
                     PYAALSTAANLGRKSLNDTMEAVREYVNGGTAPLMEATFLQAWQAELSALEPIPRMIV
                     TRRAGMHGERETLEELGAMLGVSRERVRQIEARVLEHFARRSRWRRLVEAKLLAAFGS
                     ARALPLDLLAEDAWWSGIDEQELLLDYVVRRIFEDELFVLEAPSGRRYLTKFGPNEFV
                     SHLESAKNRVAKLEYPVEVSVIDAILRAEIEALDPVLLEELREAVDALLLRDTANPSV
                     ILGYGRYRADDVIAFLNGQSEPVPVDVLEAHCGRGALPEEVLYFKRGYVGLKRHFPDF
                     DDWSHRLVPVALELMQERPSGRQWLVPEIHEALRERGLLPEWLGHWHLASLLRLSQQV
                     DYLGRLRVALKAGGHEQRVQYLELLQQIVEDAGEPLALDELLARARRRTDVPDMSAMS
                     RLNGPPFVRIDDARIGLVVRDIPGGPQAVAAAIEAVVRTLTESQVGLTPHRATVHVNG
                     ISDTHATWSRQLVTSVLRNESTIRIDRTRNIGLDAWDDARCPTRPEFIRREVQRAGGS
                     LTLSELCNSMNAFYGRVPDRGSLGLMAQQVGLGIVGETIQRTSDRGPVEAIVER"
BASE COUNT          898 a         1653 c         1631 g          908 t
ORIGIN      
        1 gacgcgctgc gctcgcagct ccgagccgca ctgcgtgaga gcacgacgcg cgtcgtgatg
       61 gtcagtgtgc gcacggttgc cgaaaaggcg cgccggatag tcttgtctct aacgagcgac
      121 tacctgcgca atccttcgcg gccggcgcct gtcttgctac gactcatgcc acagccagtc
      181 gagttccgag acctggtagc caccgcgttc gatgcgcgcc gagtcccctt cagccgcttc
      241 gacttcccgg gccttctcgc cgaaggcgcg ttccttcccg tctttactgt cgacgcgctt
      301 cgttctgccc tgaatccgag ttcgggcgcc aaagcagcgc ttcaggtgtt agcaaggggc
      361 ggcaaggtgg ttctggtcac ctcggaggag gtcaagccgg aggaactcgc gcgtcactgg
      421 ggcatgcgcg ccagcaacat tcgaacgctt cctcgcacgg cgccaccgac cggacgcggc
      481 aagggccccc tgccaccgcc ttccggaggc cagcccgtcc tccccccgcc acccggcgat
      541 cgcttcgacc gcgggagagc attgctcgtc ggcgtcgcga actatccgcg cgtttcgcaa
      601 cttcccgagt ccgtattgaa tgacgcgcgc gacatcgcgg cattgctccg gtcgccgatc
      661 cggtgcggct accccgaaag taacgtcgaa gtcttgctcg accagcaagc gaccgcccag
      721 cgattccggt tcgccttgca gcgcctggcg cagacgacga gcccggacga tacggtcgta
      781 atctacttct caggtcacgg agcacgcgaa atcaaccagg gtcgcgtgga ggcttacctg
      841 cttccgttcg acagtgaccc cgacgacctc gagcgcaccg cgctcagcgc gagcgagctc
      901 tcgaggcttc tggctgccat cagagcttcc aggctcgtcg tgctcctcga tgcctgccac
      961 gccgcgggcg ccgcccatct gaaaacagcg aaatccgcaa cgttcaagtc gggcctcgat
     1021 gacaagacat acgaagcgct cgggcgcggc accggacgcg tggtcatggc ttcgtcccgc
     1081 gctgacgaac cgtcgcgcct actccccgga atgaaaaata gtctcttcac ggcgtacctc
     1141 ctcgaagcgc tcgacggcac cgccaagaat cacggcgacg acgtcattcg cgtgctcgac
     1201 gtgttccacc acatcgcaga aaacgtgccg cgccgcgctc cccagcatcc gattttgaag
     1261 gcgcaggacg tcgagaacaa ctttgcgatc gccctcaact tgagaacgaa gcaggcgtca
     1321 cctttgccca gcgcgccgag ccacgaggtg ccgtatcgcg tcgcatcgcc attgccgccg
     1381 aaggcccgac ttgccgtcag caggggtctg gttcggcgct ggcaagaact cgccgtctac
     1441 ctcggcgtcc cgctacagga caaggtcacc ttcgatcgtg gcagggagag ccacggggtc
     1501 ctcgaatggc tagaggaacg gggtcgcttg ggtcagctga gagacgcctt tatcgatttg
     1561 ggttggaccg acttggtgga cgaattggat cgccactcga gctaacgaat ggtttggcag
     1621 agtggggatc gcgagatgtg cagcctacgc tttcggagca agacatgact cagcaaatgt
     1681 ggttttgccg cggtggggag agggtccgtg aagccttcga cgccgcgctc gcttggtgtg
     1741 aaggctgttg cgtggtgacc gagtcgccaa ccgccgccga cgcgagcgac gagctctggt
     1801 tgcagctagg tcgagcgtct gaccgcgttc gcgccgcgat cctcgcgaat atcgaagccg
     1861 cggagtccga cttgatcgct ccattcttca acgctgggcg catacgtcgt ctcgtcgatg
     1921 cgacgcatca cgaccacttc atttggttcg agcgcgacgg cgaagtgcgc tttttgctag
     1981 caccggctgc cctgcgcaag agcacgatgg atggctcggg attgtggatg tactgcgagg
     2041 gccaacgggg cgcgttaccc gaaggcttgc aaacggcgat cgaatcggtg tggtcggcgt
     2101 cggtcctgag tgagccggct gaggttctgg gcggagccgc gagcgttggg gcccccgccg
     2161 cgtattcgcg gccaattccg tctgtgcgtg aggtactcgg aattcgcacc ccgcgcgact
     2221 atttcgagct cgaagaggca gaccaagagc gcgcgctttg gagcactctc atcggggagg
     2281 ggccggtcga tctcgagcaa ggcattcggc tttgcgccga gcgtctccgg gctcagggct
     2341 ttctcgacta ccagcgcttg catcaggatg gccgcgttta ctctgcatta gaggagcgat
     2401 tgctcgccgc gcggcgccgg ctcaatttgt tcgaccgacc gcggagcggc ttcgttcgcg
     2461 cgattcaatc cgagatcggc agtgtcaccg cagagcagtg gcgagattgc ctcatgggcg
     2521 cgctggagca aggtgcccac gtggatcgag acgaggcggt gcgcctcggc ttcgaatatg
     2581 ctcaggctac ctacggtgtg aacgtccaaa ggctgcggaa cggcggccgc gccgaccagg
     2641 cgttgcgcag cgccatcaac agttcggttc gccagggata cttgcgacgg gacggcgcgg
     2701 cttacctctt gcgcgtggcg ctccagacgg agccgacgtt gcgcggggcg atcggcgtgg
     2761 gtgccgatgc gccgaacccg gcgcaggaga ccgccagctc tccgctcggg gacggcatag
     2821 aagcagctcc cgtcgcgatg aatgtcgaga cggacgcttc gacccccgct gcatccgagg
     2881 ccaccccgag ctcgccgaac acggaagcgg aatcgctccc gctcgatcgc gacgtccaat
     2941 cgttaccgct acccgctcgg accttgaatt ggtgtgcacg gtgcgaggtt tccactttgc
     3001 gtgagctcgt ggcatggcaa cccgcggcgt tcgaaaacga aaagaacgtc ggaccgagca
     3061 cggttcgtga aacgcgcgag gtcatcgagt cgctcttggg ctgcacgtgg gagcaagcgc
     3121 actcgagcgt tcacgatcga gccgtcgcga ttcgtgagca agcccaagac accgacactt
     3181 cggaggatga cgcgcccgcg gattcggtga ccgccggcgg agcggccgct tgggtccggc
     3241 tcgcagcgca gttgtctgac gaagcgcgga tgacaccgct cgtggacatt cagttgccta
     3301 ctcgcatgag gaactacgcg cagagtgagg ggctcgaacg actcggagat ttgttcagtc
     3361 tcccttacgc tgctctgtcc acggccgcga acctcggtcg caagtcgctc aacgacacga
     3421 tggaagcggt tcgagagtac gtgaacgggg gcacggctcc gctcatggaa gcgaccttcc
     3481 ttcaggcgtg gcaagccgag ctcagcgcgc tcgagcccat cccccgcatg atcgtgacac
     3541 gccgcgccgg catgcatggt gaacgcgaga cgctcgaaga gctgggggcc atgctcggcg
     3601 tgtctcgcga acgcgtgcgg cagatcgaag ctcgggtact cgaacacttc gcccgacgct
     3661 cgaggtggcg caggctcgtc gaagccaagc tgctcgcggc gttcggttct gcgcgcgccc
     3721 tgccgctcga ccttctcgcg gaagacgcct ggtggtcggg catcgacgaa caagagctcc
     3781 tactcgacta tgtcgttcgc cgaatcttcg aggacgagct cttcgtgctc gaagccccca
     3841 gcggccggag gtacctgacc aaattcggcc cgaacgagtt cgtctcccac ctggaaagtg
     3901 cgaagaatcg cgtcgccaaa ctcgagtacc ccgtcgaggt gagtgtgatt gacgcgatcc
     3961 tgcgcgcaga gatcgaggcg ctcgacccgg ttcttctcga ggagctgcgg gaggccgtcg
     4021 acgcgcttct cttgcgcgac accgcgaacc cttccgtaat cttgggctac gggcgctatc
     4081 gcgccgacga tgtcattgcg ttcctcaatg gtcagtcgga gcccgtgcca gtcgacgtgc
     4141 tcgaggctca ctgcggccgc ggcgcgctcc cggaagaggt actctacttc aagcggggtt
     4201 acgtcgggct caagcgtcac ttccccgact tcgacgactg gagccaccgc ctcgtgccag
     4261 tggccttgga gttgatgcag gagcgcccat caggccgtca atggctcgta cccgagattc
     4321 acgaagcgct gcgagagcgc ggcttgcttc cggagtggtt gggacactgg catctggcaa
     4381 gcttgctgcg actgagccag caggtcgact acctcgggcg gttgcgagtg gctttgaaag
     4441 ctggcggcca cgagcagcga gtccagtacc tggagctcct ccagcaaatc gtcgaggacg
     4501 ctggcgagcc cctcgctctc gatgagctcc tggcgcgcgc gcgccggcgc accgatgttc
     4561 cagacatgtc cgcgatgtct cggctgaacg ggccgccatt cgtgcgtatc gacgacgctc
     4621 gcatcggcct cgtcgtgcgc gacatacccg gtggcccgca agcggtcgca gccgcgatcg
     4681 aagcggtggt gcgcaccctg accgagtctc aggttgggct cactccgcat cgagccaccg
     4741 tccacgtcaa cggcatttcg gatactcacg cgacttggtc gcggcagctc gtcacgtccg
     4801 tcctgcgcaa cgagagcacc atccgcatcg atcggaccag gaacatcggc ctcgacgcat
     4861 gggacgacgc tcgctgtccg actcgccccg aattcatccg ccgagaagtt caacgcgcgg
     4921 gcggaagcct caccctgtcc gagctctgca acagcatgaa cgccttttac ggacgcgtgc
     4981 cggaccgagg gagcctcggc ctcatggcgc agcaggtagg gctcgggatc gtgggcgaaa
     5041 ccatccagcg aaccagcgat cgcggccccg tcgaagcgat cgtcgaacgc
//
LOCUS       DASTKS010000180         4998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_475032, whole genome shotgun sequence.
ACCESSION   DASTKS010000180 DASTKS010000000
VERSION     DASTKS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475032"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFQ35_03770"
     CDS             complement(<1..294)
                     /locus_tag="VFQ35_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET9929774.1"
                     /translation="MAEYREFQDGELIAGTRYQVQRLIGVGGMGTVYEVEHIELGKRF
                     VLKALLRELSRREDLVIRLRNEWRALGRLEHPSIVTVTDAGTSATGVPFYVMER"
     gene            complement(472..993)
                     /locus_tag="VFQ35_03775"
     CDS             complement(472..993)
                     /locus_tag="VFQ35_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET9929775.1"
                     /translation="MTPPSVAVRASQGGPWYPGGGAPPPGAPAGGGGPGGYGGGGYGG
                     PGGGGYGGGGYGGGGYGGGPPPKPITGSPETMALHAMTIDPRTGLPRGEKPPASTASV
                     VALVCGILLCLGPLTGLSAIIAGFVGLKAARTRPQEVGGAGLASVGIGLGTLNLLISI
                     IAAVYVALSLVSR"
     gene            1072..1365
                     /gene="gatC"
                     /locus_tag="VFQ35_03780"
     CDS             1072..1365
                     /gene="gatC"
                     /locus_tag="VFQ35_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9929776.1"
                     /translation="MAITRETVLHVARLSRLDLAEDEVERMQRDLDRILEYVEELNTL
                     DTSQVAETAHAAVDAAPLREDAVRPGVQNERAMAEAPRSSGGAFAVPAFVDES"
     gene            1362..2858
                     /gene="gatA"
                     /locus_tag="VFQ35_03785"
     CDS             1362..2858
                     /gene="gatA"
                     /locus_tag="VFQ35_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET9929777.1"
                     /translation="MSDLDLSIPALVDRVKSGAVSPVALAEESFARIRKHENLNAFLN
                     LSEESALRAAREVESAQKKGVSLGKLAGVPIAIKDALCTIDAPTTCASKILTRASADA
                     SGGTSPERGFRPPFDATVVARLRAAGAILVGKTNMDEFAMGSSGENSAFGPPRNPHDP
                     TRTGGGSSGGSAVAVAAGMAAGSLGSDTGGSIRQPAALTGCVGVKPTYGRVSRYGLIA
                     FASSLDQVGPFARDVRSAARILEVIAGRDPNDSTSSARPVGDYEGACEREAKGLRLGV
                     PEEYFGEGLDPDIKHSIKDAIARLEREGVSVRPVALPHTRYGIATYYIIATAEASSNL
                     ARFDGVRFGLRLEPTRGDLNSMYGRTRDRGFGAEVKRRILLGTYVLSAGYYDAYYRKA
                     QRVRTLIKQDFEKAFENVDIIAVPTTPSPAFRFGEKVDDPLAMYLADVYTLPASLAGI
                     AGLTVPVAPTPARADRPSLPVGLQLLAPAFEEERLFTLASHVERLVRG"
     gene            2855..3463
                     /locus_tag="VFQ35_03790"
     CDS             2855..3463
                     /locus_tag="VFQ35_03790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9929778.1"
                     /translation="MSTLTFGPAAPSDAAALEAFFERCEVACRCRYWHFTGDKNAWLN
                     RCFHEPADNARELRDDVEHSRNLLGIVARDGDSIRGWMKITPASAVDKLYQQRLYKNL
                     PCFAGNRDRIFTIGCVLIEPDFRHRGIANRLLAAAIEAARAENARGIEAFPRRGEHLG
                     DAELWMGPISIFERAGFQVVNDFAPYPVLRLELGEPHPTTST"
     gene            complement(3480..4307)
                     /locus_tag="VFQ35_03795"
     CDS             complement(3480..4307)
                     /locus_tag="VFQ35_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9929779.1"
                     /translation="MPLPSKYEVDPADPRAPSVEVWHSLSRSEQNRIVEQLPSEVELG
                     PPEGDPHRIPKQRGIEALDAFFRRAGRRVYLSSELPVYYPAERMFAPDLIAVCDVEPH
                     ERLRWVVTHEGRGLDFALEVTLSGSRKKDLEENVARFARLGIPEYFVFDRAQRRIFGW
                     QLVAPGRPYEPIVPQQGRWRSGVLGLDLAVEGDRFRFYWGTAVVPELAELVARANSLV
                     DELQDKLEHLDEQFAREQQRAEQEQQRAEQERQRAEQAEAELERLRAELERLRRERQ"
     gene            4543..>4998
                     /locus_tag="VFQ35_03800"
     CDS             4543..>4998
                     /locus_tag="VFQ35_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9929780.1"
                     /translation="MAGAKHSHSGTISSRRFLPLLVAPTADGKVSNSIRQGLVPVGCF
                     TVAAPGFDFDSSLLLPKMAKEFGKLGALLASLPEHPVSVFGHADPTGSDDYNKELAGR
                     RALSVYGLLTKDPDVWEKLYASGSKAKGDHWTQRQLLVMLSALESAETGE"
BASE COUNT          865 a         1713 c         1579 g          841 t
ORIGIN      
        1 gcgctccatc acgtagaagg gtacgcccgt cgcggaggtg cctgcatcgg tgaccgtgac
       61 gatgctggga tgctcgagac gaccgagcgc gcgccactcg ttgcgaaggc gaatcaccag
      121 gtcttcgcgc cgcgaaagct cgcgcagcaa ggccttgagc acgaagcgct tgccgagctc
      181 gatgtgctcg acctcgtaga cggtgcccat cccgcccacg ccgatcaggc gctgcacctg
      241 atagcgggtt ccggcgatga gttccccgtc ttgaaactca cgatactcag ccacgttgtt
      301 tggaaccctc ggcgtacatc tcggacgcgg tgtcggcagc gtgggcgcga gcataacgtc
      361 gggcatctcg gccgtcgagg cagtgttttc ttccgcgtgc actcacagtc gtcggcacgg
      421 atgccgcgcc ggttgcgcgc gtacgcaaat ctctcgcaat ggcgagggaa ctcagcgcga
      481 caccaagctg agggctacgt acacagccgc gattatcgaa atgagaagat tgagtgtacc
      541 gaggccgatc cccaccgaag caaggcctgc gccgccaact tcttgaggtc gcgtccgcgc
      601 ggccttgagc ccaacgaagc cggcaatgat tgcagagagc cccgtaagcg gccccaaaca
      661 cagcagaatc ccacagacca gcgcgaccac cgaggccgtg ctcgcgggcg gcttctcgcc
      721 acgcggtaat cccgtacgcg gatcgatcgt catggcgtgc aaggccatgg tctcgggcga
      781 acccgtaatc ggtttgggtg gaggtccgcc tccgtaacct ccgcctccat aacctccgcc
      841 tccgtaacct ccgcctccgg gacctccgta acctccgcct ccgtagccgc ccgggccacc
      901 gcctccggcc ggagcaccag gaggaggggc accaccgccc gggtaccagg gcccgccctg
      961 agaggcccga accgcaacgg agggaggcgt cacgccggcg aggataactg aaaggaggca
     1021 gtgacgtggg cgtttgtcga atcggcctcg ctgatttagg ctgcgccgcc catggccatc
     1081 acccgcgaaa cggttctgca cgtcgcgcgc ctttcgcgac tcgacctcgc cgaagacgaa
     1141 gtcgaacgca tgcagcgcga tctcgaccgc atcctcgagt acgtcgaaga gctcaacacc
     1201 ctcgatacgt cgcaagtggc ggagacggcg cacgctgccg tcgacgcggc accgctgcgt
     1261 gaagacgcgg tgcgccccgg cgttcagaac gaacgggcca tggctgaggc tccgcgcagc
     1321 agcggcggag cgtttgcggt tccagctttc gtggacgagt catgagcgat ctcgatcttt
     1381 cgattccggc cttggtagac cgcgtgaaaa gcggcgcggt gagccccgtc gcgctcgccg
     1441 aagaatcctt cgcgcgcatc cgtaaacacg agaacctgaa cgcgtttctc aacctcagcg
     1501 aagagagcgc gctccgtgcc gcgcgtgagg tggagagcgc ccaaaagaag ggcgtttcgc
     1561 tcggcaagct cgccggggtg cccatcgcca tcaaagatgc gctctgcacg atcgatgcgc
     1621 ccacgacgtg cgcttcgaag atcctgacgc gcgcttctgc cgatgcttcg ggcgggacgt
     1681 caccggagcg cggtttccgt ccgcccttcg atgcaaccgt ggtcgctcgt ctgcgcgccg
     1741 ccggcgccat cctcgtcggc aagacgaaca tggatgagtt cgcgatgggg tcgtcgggcg
     1801 aaaacagcgc gttcggtccg ccgcgcaacc cgcacgatcc gacgcgcacg ggcggaggct
     1861 cgagcggcgg cagcgccgtg gccgtggccg ctggcatggc cgcgggctcg ctcgggagcg
     1921 acaccggcgg ctccatccgt caacccgccg cgctcaccgg ctgcgtcggc gtcaaaccga
     1981 cgtacggtcg cgtttcgcgc tacgggctca tcgccttcgc gtcgagcttg gaccaagtgg
     2041 gccccttcgc gcgggacgtg cgcagcgccg cccgcattct ggaagtcatc gcgggacggg
     2101 atcccaacga ttccacgagc tcggcgcgcc cggttggcga ctacgaaggc gcctgcgagc
     2161 gcgaagccaa gggcctgcgc ctcggcgttc ccgaagagta cttcggcgaa gggctcgacc
     2221 cggacatcaa gcacagcatc aaagacgcga tcgcgcggtt ggaacgcgaa ggtgtcagtg
     2281 tccgccccgt cgccctcccc cacacgcgct acggcatcgc gacctactac atcatcgcga
     2341 ccgccgaagc ctcgagcaac ctcgcgcgct tcgacggcgt gcgcttcggc cttcgcctcg
     2401 agcccacccg cggtgatctc aactcgatgt acggacgcac ccgcgaccgc ggcttcggcg
     2461 ccgaggtgaa gcgacgcatc ttgctcggca cctacgtgct cagcgccggc tactacgacg
     2521 cctactatcg caaggcgcag cgcgttcgca ccctcatcaa gcaagacttc gagaaggcat
     2581 tcgagaacgt cgacatcatc gccgtcccga cgaccccgag ccccgctttc cgcttcggcg
     2641 aaaaggtcga cgacccgctc gcgatgtacc tcgccgacgt gtacacgctc ccggcgagcc
     2701 tcgccggcat tgccgggcta accgtccccg ttgcgccgac tcccgcgcgc gccgatcgtc
     2761 cgagcctccc ggtggggctc cagctcctcg cgccggcctt cgaagaggaa cgcctcttca
     2821 ccctcgcttc gcacgtcgag cgcttggttc gcgggtgagc acactcacgt tcggccccgc
     2881 ggcaccttcc gatgcggcag cgctcgaagc ctttttcgag cgctgcgaag tcgcgtgccg
     2941 ttgccggtac tggcacttta ccggcgacaa gaatgcgtgg ctgaaccgct gctttcacga
     3001 gccggccgac aacgctcgcg agctccgtga cgacgtcgaa cactcgagga accttctcgg
     3061 catcgtcgcg cgagacggtg actcaatccg cggctggatg aagatcacgc ccgcgagcgc
     3121 cgtcgacaaa ctctaccagc agcgcctcta caaaaacctt ccgtgcttcg ccgggaaccg
     3181 cgaccgaatc ttcacgatcg gctgcgtgct catcgaaccg gactttcgtc atcgaggcat
     3241 cgcgaatcgg ttgctcgcag ccgccatcga agccgcccgc gccgagaacg cccgcggcat
     3301 cgaagccttc ccccgccgag gggaacatct cggcgacgcc gaattgtgga tgggcccgat
     3361 ctccatcttc gagcgcgccg ggttccaagt cgtgaacgac ttcgctccgt acccggtgct
     3421 gcggctcgaa ctcggcgaac cccacccgac cacttcgacc tgacctcccg tccgcgcgcc
     3481 tattgccgct ctctccgcag tcgctcgagc tcggcccgca gccgctcgag ctccgcctcg
     3541 gcctgctcgg cgcgttgccg ctcctgctcg gcgcgttgct gctcctgctc ggcgcgttgc
     3601 tgctcccgtg cgaactgttc gtcgaggtgt tcgagcttgt cttgaagttc gtcgacgagc
     3661 gagttcgccc gagcgaccaa ctcggcgagc tccggcacga ccgcggtacc ccaatagaag
     3721 cgaaagcggt ctccctcgac cgccaaatcc aacccgagga cacccgaacg ccaacgcccc
     3781 tgttgcggaa cgatcggctc gtagggacgc cccggcgcga cgagctgcca gccgaagatg
     3841 cgtcgctgcg cgcgatcgaa aacgaagtac tccggtattc cgagacgcgc gaagcgcgct
     3901 acattctcct cgagatcctt tttgcgactt ccggagagcg ttacctcgag cgcgaaatct
     3961 agcccccgcc cctcgtgcgt gacgacccac cgtagtcgct cgtgaggctc gacgtcgcac
     4021 acagcgatca gatcgggcgc aaacatgcgc tccgccgggt agtagactgg cagctcgctc
     4081 gagaggtata ccctgcgccc cgcgcgccga aagaaggcat cgagcgcctc gatgccgcgc
     4141 tgcttgggga tccggtgggg gtctccttcc ggcggcccca actcgacctc cgacggcaat
     4201 tgctcgacga tgcggttttg ctcgctgcgg ctcaggctat gccaaacctc gacgctaggc
     4261 gctcgtggat ccgccgggtc gacctcatac ttcgacggaa ggggcattgc gaaatcctaa
     4321 catgccccac ttgcgaggcc agagccgctc cgcgtcgcat gcggcgccga ccacggcgca
     4381 gttcccgccg gctcgggttt agcggaagga ttcggtagca tttgacgccg agatcgcgag
     4441 atgaaatcgt ggggctggga tggagttcgg tatgagtgag gcctgggtaa tggctggggt
     4501 acgggaccgt ggcgctcggg tggcgaggat caggaggttc tgatggcggg ggcaaagcat
     4561 tcgcacagcg ggacgatttc ttctcgtcgt ttcttgccgc tcttggtggc gccaacggcg
     4621 gatggaaagg tttcgaattc gattcgccag gggctcgttc cggttgggtg cttcaccgtc
     4681 gcggcgccgg ggttcgattt cgactcgtct ttgttgttac cgaagatggc gaaggagttc
     4741 ggcaagctcg gagcgctctt ggcgagtctc ccggagcatc ccgtttcggt gttcgggcac
     4801 gcggatccga ccgggtccga cgactacaac aaggagctcg cagggcgtcg ggcgctgagt
     4861 gtctacggct tgctcacgaa ggatccggac gtttgggaaa agctttacgc ttcggggagc
     4921 aaagcgaagg gcgaccactg gacgcagcgg cagctcctcg tgatgttgag cgccctcgag
     4981 agcgccgaga ccggcgag
//
LOCUS       DASTKS010000181         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_250068, whole genome shotgun sequence.
ACCESSION   DASTKS010000181 DASTKS010000000
VERSION     DASTKS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250068"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFQ35_03805"
     CDS             <1..259
                     /locus_tag="VFQ35_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929781.1"
                     /translation="CSGIPTSTQPEVIVQLPRAPGTYSLSLQRNATFAIQRSTTTDNL
                     VATKGKLVIDEVTATTIKGGAYIEYNAENSVNGTFEASICP"
     gene            454..1032
                     /locus_tag="VFQ35_03810"
     CDS             454..1032
                     /locus_tag="VFQ35_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET9929782.1"
                     /translation="MSVFFIAYSTILVAELIGDKTLYTLSALATRFRLAPIIAGAALA
                     FALKMAAAVLLGHAISRLPVWLVTALSGATFIGMGIGIFLSLSKPASEPPSSSGKSEP
                     FTRGSVTAFTAIFLPEWGDPGQLAAALLAAQGHSSALVWGAGTLAMTTKALLAGVLGL
                     GLRRVVPITKMRWASAVMFVTLGILALFRVEL"
     gene            1037..1390
                     /locus_tag="VFQ35_03815"
     CDS             1037..1390
                     /locus_tag="VFQ35_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9929783.1"
                     /translation="MKRRQRYVFVCTKRRPDDHKKGSCAVHGSEELLRTLKQAAVAAK
                     VDAKVTSSGCFDLCWVGASVAVMPDNVFLKRIELSDVERVVGALALETKAELEEALAE
                     HVAGERDFIDPNKAG"
     gene            complement(1468..2346)
                     /locus_tag="VFQ35_03820"
     CDS             complement(1468..2346)
                     /locus_tag="VFQ35_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929784.1"
                     /translation="MFRVDGRGSLLLILAAACVGLRAEPALAAAGSDATTSTSAPTPM
                     SSTLMARPDERRANETTPLEQPGQRALDEVPRVGVVESAFGSPRRTIFAGAEGLLIPK
                     ASESGQGGFFFGGSPLPRLTLYGMLGRDGEGRFAPSATAQYAFLGAEGAQHALAALVQ
                     YKTEGFTEAGGELELGLLTGFHRARWLLNVGAVAGFGLEEEDEGEMDAEGKLRVAYDV
                     LDSVRVGVLARFRERLAGERRLTGDRNWDFIGGPEVAGSFGPFMLSASGGPTTFGVPD
                     GVGAYGMLTLAAVSRL"
BASE COUNT          462 a          842 c          797 g          402 t
ORIGIN      
        1 atgctcaggc atcccgacgt ccacccagcc cgaagtcatc gtccagctcc cacgcgcacc
       61 tgggacgtac tcgctcagct tgcaacgcaa cgcgaccttc gcaatccagc gctccactac
      121 gacggataac ctcgtcgcga ccaagggcaa gctcgtgatc gacgaagtca ccgcgaccac
      181 aatcaagggc ggcgcgtata tcgagtacaa cgccgagaac agcgtgaacg gcaccttcga
      241 agccagcatt tgcccctagg aactgccgca aggatcggga cgcgggcgcg ccttgccgac
      301 acgattgcct ccgcccaaaa ggatggtaac ttcgggctga gttgggaggt tggccgtcca
      361 cgtgggcaac atcgggagtg atcgggctga aggaacgcgg cggagcttgc gtcattgcga
      421 cgggcgcccc tgcgcactag ggtcgtgacg cgcatgtcgg tgttcttcat cgcttattcg
      481 acgatcctgg tcgcggagct catcggagac aaaacgctgt acacgctgag cgccctcgcg
      541 acgcgctttc gtttggcgcc gatcatcgcc ggcgcagcgc tcgcgttcgc gttgaagatg
      601 gcggccgcgg tgctgctcgg tcacgcgatt tcacggctgc ccgtttggct cgtcaccgcg
      661 ctgagcggcg cgaccttcat cggcatgggg atcggcatct tcctttccct atccaagccg
      721 gcgagtgaac cgccctcttc cagcgggaag tccgagccct tcacgcgcgg ctccgtcacg
      781 gccttcacgg cgatcttcct gccggagtgg ggcgacccgg gacagcttgc agcggcgctg
      841 ctcgccgcac aaggccactc gagcgcgctc gtgtggggcg ctggcacgct cgcgatgacg
      901 acgaaagcct tgttggcggg agtgctcggg ctcggcttgc gccgcgtcgt cccgatcacc
      961 aaaatgcggt gggcctcagc cgtgatgttc gttacgctgg ggattttggc cctgttccgc
     1021 gtagagctct gagctgatga aacgacgaca acgctacgtg ttcgtttgca ccaaacgccg
     1081 acccgacgac cataaaaagg gctcgtgcgc cgtacacggt agcgaagagc tcttgcgaac
     1141 gttgaagcaa gcagcggtgg ccgcgaaggt ggacgccaaa gtgacctcga gcgggtgctt
     1201 cgatctgtgt tgggtgggcg cgtccgtcgc ggtcatgccc gacaacgttt ttctgaagcg
     1261 catcgagcta tcggacgtgg agcgcgtcgt cggcgcactc gcactcgaga ccaaggccga
     1321 gctcgaagag gcgctcgcgg aacacgtcgc cggtgaacga gacttcatcg accccaacaa
     1381 ggccggctaa ggcaaccggc taaggcgtgg ctaagtttcg ggcgcgggcc gccccacgct
     1441 cagcgctgtc ggaggttcag ctcggattca caggcgtgac accgcagcca gcgtgagcat
     1501 gccgtaggca cccacgccgt ccggaacgcc gaaggtggtg gggccacccg acgcgctcag
     1561 catgaagggg ccgaagctcc cggcgacctc cgggccaccg atgaagtccc agtttcggtc
     1621 gccggttaac ctgcgctcgc cggccaggcg ctcgcgaaac cgagccaaaa cgccgacgcg
     1681 cacgctgtcg agcacgtcgt acgcgacgcg cagctttccc tccgcgtcca tctcgccttc
     1741 gtcttcctct tcgagaccga acccggcgac agcgccgacg tttagcaacc aacgggcccg
     1801 atgaaacccc gtgagcagcc cgagctcgag ctccccgccg gcttcagtga agccctcggt
     1861 cttgtactga accagagctg ccaaggcgtg ctgcgcccct tcagcgccga ggaaggcata
     1921 ctgcgccgta gcgctgggag cgaagcggcc ctctccatcg cggccgagca tgccgtacag
     1981 tgtgaggcgt gggagcggcg aaccgccgaa aaagaaccct ccctggccag actccgacgc
     2041 ctttggaatc aatagtccct cggcgccagc gaaaatggtg cgccgaggcg agccaaaggc
     2101 gctctccacc acgccgacgc gcggcacttc gtcgagggcg cgctgcccgg gctgctcgag
     2161 cggcgtggtc tcgttcgccc gccgttcatc gggccgagcc atcaaagtcg acgacatcgg
     2221 cgtaggcgcc gaagtgctcg tcgtggcgtc cgaaccggcc gccgccaaag cgggctcggc
     2281 acgaagaccg acgcacgccg ccgccaaaat caacaacaag cttcctcgac cgtccaccct
     2341 gaacatcgtc atcgcatata gcagtatcgg cttgcggctt ctagcgcctc gaggagcgag
     2401 gggggcgccg cgccgtcgac aggagaccgc agcgcgactc cacgcaatgt ctgcggttcc
     2461 gcgcgaaagc tagggcgcac accggaaagg cgacgcgagt cgg
//
LOCUS       DASTKS010000182         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_919925, whole genome shotgun sequence.
ACCESSION   DASTKS010000182 DASTKS010000000
VERSION     DASTKS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919925"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFQ35_03825"
     CDS             complement(<1..764)
                     /locus_tag="VFQ35_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9929785.1"
                     /translation="MSTALLDADFLRRLEALRRRLTSEARSGESGESVAPRRGTGAEF
                     REHRPYAPGDDPRRIDWMAYARSGEPLIKLFHHEEDRILRLLVDASASLGFGTPSKLE
                     LSQRLAAALGYLALSGSERTQVFVARNEESKPLGAIGNSHRGRRAFGELCRELAALRP
                     EGKADLSRAIESTVARSVRPGVLAVLSDFLDPGGVLVALSRARAAGHDVLLVQILSQD
                     ELEPSFEGDLALQDSETGVVLDITADASAISAYLDRL"
     gene            complement(761..2404)
                     /locus_tag="VFQ35_03830"
     CDS             complement(761..2404)
                     /locus_tag="VFQ35_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929786.1"
                     /translation="MSVQTHTSSLPPPQGGLGARISWLFARLLSNPNPVWIRELRQSA
                     RLTRTPVILAAITSTMTLLIASIGGVLSATSEPAQVGIGVFHTFFSLAFAVVTWVGPA
                     VAAATIASERSGRTWETLLLTGLSPAAIARGKFLAALTYVALYIVMLAPVGGLSFLFG
                     GVTPAEVVLAFALLLLIAGLSVAFGLAVSSKLSSPSVSILVTLLVAVPLSIVVYTAGG
                     VGLSLVARDQWPGITGGAPVWLPTAYVRADFSLAYLVFLVLFPLIAAVVPAWLFYEVT
                     IANMASASDDRSTRLRVWLLACGPLLGIANVLAALAMTKSEWCFAGQITCWLFSLFGA
                     FLVAGEPLGPSLRVETHWGRAHASALRRAVGPGVGRAAGAVVLLTLVCQTAVTAVGVL
                     LARSRDDRLALLGFGAYAIGFSLFCVGFAAWARSRTSAGSLPRVLLISATLLALLGPY
                     IAMAIGGMFAEDNLKMRLVAAPSPAFAFAAYERFQSPGGSAELYGLAGAGAALGWALI
                     GLGLLALGATRARQRWARERAVAANPAPAAAPVANAAGA"
BASE COUNT          501 a          842 c          875 g          339 t
ORIGIN      
        1 agccggtcga ggtacgccga aatcgcgctc gcgtccgccg ttatgtcgag gacgactcct
       61 gtttctgagt cttgcagggc gagatccccc tcgaaggacg gctcgagttc atcctgactc
      121 agaatttgga ccaagagcac gtcgtgcccc gcggctcgtg cacgtgacag cgcgacgagc
      181 acgccgcccg ggtccaaaaa atcactcagg actgcgagca ctccgggacg aaccgagcgg
      241 gccacggtgc tctcgatagc gcgcgagagg tcggctttgc cttcgggacg caacgccgca
      301 agctcgcgac acaactcgcc gaatgcgcgg cgccctcggt gtgagttgcc gatggctccg
      361 agtggcttgc tctcttcgtt tcgcgcgacg aagacctgcg tgcgctccga cccagatagg
      421 gccaggtacc cgagcgctgc cgccaagcgt tgcgacagct cgagcttgct cggcgtgccg
      481 aatccgagcg acgccgacgc atcgacgagc aggcgcaaga ttcggtcctc ttcatggtgg
      541 aaaagcttga tgagcggttc gccagagcgc gcataagcca tccagtcgat gcggcgggga
      601 tcgtcgccag gtgcatacgg acggtgttcg cggaactcgg cgccggtgcc tcgccgcgga
      661 gcgacgcttt cgccgctttc cccggagcga gcttccgagg tcaggcgccg tcgcagtgcc
      721 tcgaggcgcc gcagaaaatc agcgtcgaga agggcggtgc tcatgctccc gcagcgttgg
      781 cgacgggtgc cgctgcaggc gccggattcg cggctacggc gcgttcccga gcccagcgct
      841 ggcgtgcccg agtcgcgccc aaagcgagca gaccaaggcc gatgagcgcc cagccaaggg
      901 cagcgccggc gcccgcaagc ccatagagct ccgcgcttcc gcccggggac tggaagcgct
      961 cgtaggcagc gaaggcgaac gccggagagg gcgccgcgac caatcgcatc ttcagattgt
     1021 cctcggcgaa catgccgcca atcgccatgg cgatgtacgg cccgaggagc gccaacaagg
     1081 tcgcgctgat gagcaggacg cggggcagtg agcccgcgga cgtccgcgag cgggcccagg
     1141 cggcaaatcc gacacagaaa agcgaaaagc cgatcgcgta agcgccgaac ccgagcaggg
     1201 ccaagcggtc atcgcggctt ctcgccagca agaccccgac cgccgtcacc gccgtttgac
     1261 agaccagcgt gaggaggacg acggcacctg ccgcgcgccc cacgccgggg cccaccgcgc
     1321 ggcgcagcgc cgaagcgtgg gcgcgccccc agtgggtctc gactcgcaac gaggggccca
     1381 atggctcgcc cgcaaccaga aaggctccga agagcgagaa cagccagcag gtgatttggc
     1441 ccgcgaaaca ccactccgac ttcgtcatcg cgagtgcagc gagaacgttc gcgattccga
     1501 gcagtgggcc gcacgcgagc agccacactc gcagtcgcgt cgagcggtcg tcgctcgcgg
     1561 aagccatgtt cgcgatcgtg acttcgtaga agagccaggc ggggacgact gccgcgatga
     1621 ggggaaacag aacgagaaag accaggtacg cgaggctgaa gtcggcgcgc acgtacgcgg
     1681 tcggtagcca cacgggcgcg ccgccggtga tgcccggcca ttggtcgcgg gcgacgaggc
     1741 tcagtccgac gccccctgcc gtgtagacga cgatcgagag cggaacggcg acgaggagcg
     1801 tgacgaggat ggaaaccgat gggctgctca gcttggagct cactgcgagc ccgaacgcca
     1861 cggagagacc ggcaatgagc aagagcagtg cgaacgccag caccacttcg gcaggcgtca
     1921 cgccgccgaa aaggaacgac agcccaccga ccggcgcgag catgacgatg taaagcgcca
     1981 cataggtaag cgctgccaag aactttccgc gtgcgatggc agccggcgag aggcccgtga
     2041 ggagcagagt ctcccaagtg cgcccactgc gctcgctcgc gatggtggcc gcggcgacgg
     2101 caggccccac ccaggtgacg acggcaaacg cgagcgagaa gaacgtgtga aatacgccga
     2161 tgccaacctg cgccggctcg gacgtcgcgc tcaagacgcc gccgatcgac gcgatgagca
     2221 gcgtcatcgt gctcgtgatc gcggcgagga tcaccggggt gcgcgtcaga cgagcggact
     2281 gtcgaagctc ccggatccac accggattcg ggttgctcag gagtcgcgcg aagagccacg
     2341 aaatccgtgc cccgagcccc ccctgtggag gcggcaacga ggacgtgtgc gtttggacgc
     2401 tcattgcagc tctccgcggg tgacttcgag gaagatgcgc tcgagctcgt tgcgctcggg
     2461 ctccactccc accacgccga cgccgttcga gacgaggtgt cggactacgt cggcaacgaa
     2521 ctggtcggtc cccgtgtaaa ggacggtgag ctgcccg
//
LOCUS       DASTKS010000183        12676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_475100, whole genome shotgun sequence.
ACCESSION   DASTKS010000183 DASTKS010000000
VERSION     DASTKS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475100"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..963)
                     /locus_tag="VFQ35_03835"
     CDS             complement(397..963)
                     /locus_tag="VFQ35_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929787.1"
                     /translation="MKQTSLAPSLSSCSSRLGARCAGALFAALAVFVAAPSTAHAAPG
                     SRLGVDLDYINDINEDFSEAGKGVALRYGYKLDLLVLSVTPEVGLGGYWFGGAADPRI
                     LQGFVGGRATFGKVLEPGVFAHLGYGSMKVLDESRGGTSIDAGVTLDLTMLPFIDLGV
                     HAAYDAFLVKDEDAFDWFRVGAHAALAF"
     gene            complement(1039..2199)
                     /locus_tag="VFQ35_03840"
     CDS             complement(1039..2199)
                     /locus_tag="VFQ35_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929788.1"
                     /translation="MTDTRLQRLEALLTRVRRRAAEARGAFGAAPPAPGAPAPHAAPT
                     PVPPAEDPVTIPPPFSRQAPAPAARSLPVQAPESGWPEAPEQVADVMVARRVSDVAEL
                     EVVENGPADALGEEDLLEIPSDMLESIPPGAVVEEIDEEPPASSKRPRVAHLDELDEG
                     VALAADEAREVPIKTPPPESGPQASPPASLGAPPAPDVEHLLDQEISPPPAAAADQFT
                     APFDSLEQPFGTRPPPPASGPTVEQLGQTIDLEESGGPELELDRPLISAAPLESHPDD
                     LEAALPAQESAGAYHEALMPPPQAERELEEHRERHGAPLPEMPSAIPEVSVVGAPPLA
                     TMPAASTAGELNQLIQRPALGALDVVTVSGVASKPEPRSFLQLLDASIALGG"
     gene            complement(2196..2756)
                     /locus_tag="VFQ35_03845"
     CDS             complement(2196..2756)
                     /locus_tag="VFQ35_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929789.1"
                     /translation="MPTLPFDPTAAVRFDLARGQIAVPNSGERVLIPADALLTLCDAV
                     DTDARKDFARRLGTEAGRRTAERLGSTDSVNPESVVEHLGGDLALMGLGSLELERWGR
                     ALVLAFRGSPFGARGDDLLAAALEGAIQRAFGRDVGVVRLARDDDRARFLVTSRAGAE
                     RVKSSIESGRAWGEVLAELDSKTGAA"
     gene            2844..4208
                     /locus_tag="VFQ35_03850"
     CDS             2844..4208
                     /locus_tag="VFQ35_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929790.1"
                     /translation="MPRAAAIAAWVTAALVSMTASGAMPRREPPLILGGALPRPKAEV
                     IVDPRLPTGARVGAAPRPTATASACSSRRPVCVHAFSQQGARLEAALDALERAYERLV
                     YALGMPAPLSDLGLGGTDALDAYVGGEALLVEADPPKLGPFTESSGWCELPSVDGALL
                     DRAATQCVGEAIALRLDASEPPHLRRAFATWLWWLVGAPTSLDLEAIDLVQAHPERPI
                     ASRELSAESEGDAMFFEYLESARSTRPWGELSAALFGTAVSDNRKLGLYYDNEPDLFD
                     VLRHSLNEERDRFAALMVDFAVARAFVGSRDDGLHLPNLAFSGDFGRVRYDWLVKFST
                     LPRRVLVNPPIDSTGAVVIWLDLDEVGLGAALGFHADWEPPVSFHWQLVKVGPNGDEI
                     ARIDVPFQERGHEVDARLTNLDGASAILIVGTNLEGIDLSHPFDPDVSPFEPHGATVY
                     VVRM"
     gene            complement(4212..5114)
                     /locus_tag="VFQ35_03855"
     CDS             complement(4212..5114)
                     /locus_tag="VFQ35_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9929791.1"
                     /translation="MLPRSRVLLALSVLAASCSPKPSVLARPTGPMSLEEAREYVLAL
                     VNRDRRDNGLAEVEHDAAAERAGQVHAEDMATFSYTAHWGTDGSVPEERYTLAGGEDF
                     VQENAGCFADNQKRALVQNPSFSAEDLEKIEAAFMAERPPADGHRRNILKDTHTHFGV
                     GLARPEGVPGACMSQEFVDERGDYDDLPKSARVGSKITVEGEVKDPVEFGGVGLSRIE
                     PRKPRSARELNRTNTYPIPEPYILYFPKGFKTPKEVKLDGKHFSIEVPLGAGRGRYGV
                     SIWGKYPGSGNQLVMVSLRTIDVR"
     gene            complement(5171..6604)
                     /locus_tag="VFQ35_03860"
     CDS             complement(5171..6604)
                     /locus_tag="VFQ35_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929792.1"
                     /translation="MTSPLRTTDKLDTHQKALAVNLDARRYGTFAEIGAGQEVVRWFF
                     RVGGAAGTIAKSISAYDMAVSDAIYGRTQRYVCRQRLESMLAYEHKLNLERLRDARGD
                     TTSFFVFADTVAAKSFKGTNECHGWLGVRFQAHPRDQDSQVIIHVRLLDNEMALQQEA
                     LGVVGVNLLYGAFFMHHDPDRLIESLLDNLSTDRIEIDMIEFSGIEFRHVDNRVMALK
                     LVQLGLSGAAIFDPKGEVLQPSELLRKSPVLVERGAFLPVTRVHIDMLTSARKKFVKE
                     GASGDERIVELMEINMRNLIESGQGGVDLRDFLARADVLAAAGKTVLISDYFEYYRLA
                     GYLRRYTQRPIALVLGAGSLSSLFDEKYYTNLDGGILEAFGRLFKNDTKLYVYPSLDP
                     GREKLVTASNFTPNPELARLYGHLLDRGCIVELDEFDRDCLNIFSNDVLRMIRSGDAN
                     WERQVPPEVIDVIKKGSFFGHRRARDS"
     gene            complement(6810..7985)
                     /locus_tag="VFQ35_03865"
     CDS             complement(6810..7985)
                     /locus_tag="VFQ35_03865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9929793.1"
                     /translation="MGFPLNVVVASAARTAVGRALKGTLRNTRPDDLAAYAMVAAVER
                     VKGLDPAALDDVVLGCAMPEGEQGLNVARNAVFLARFPNSVPAETINRFCSSGLQALV
                     HGAMAIQTGLAEVVLAGGVESMSMVPMSGNKVSLNPKLMDTYPEAYISMGHTAERVAA
                     RFSVSRADQDAFAVLSHERALEAITAGRFTQEIVPVPARVFEGNGVAEMTFAVDELPR
                     AGTTLDVLAKLKPAFSQAGSVTAGNSSPLSDGAAAAVLMSEKRASDLGVRPLGRLRAY
                     AVAGVDPDIMGIGPIPAIRKVLKLTGLDIKDIDLFEINEAFAAQAVYCQRELGIPKEK
                     LNVNGGAIALGHPLGCTGAKLTATLLHELGRRGGRYGIVSMCIGGGMGAAGLFEVGG"
     gene            complement(7996..10401)
                     /locus_tag="VFQ35_03870"
     CDS             complement(7996..10401)
                     /locus_tag="VFQ35_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9929794.1"
                     /translation="MAEPLHDPVNMAAERIRTAGVIGAGVMGSAIAAHFAGAGIRTHL
                     LDIVPPNLKPEERTQPKARNRFAEGGLERALKAKPALFFDPDAARLVQTGNLEDHLER
                     LRECDLVVEAVVENLDVKQKLFATIQPYLSEQAILASNTSGLSVAALSNVLPEALQSR
                     FVILHFFNPVRYMRLLEVAPGPHTEPTVLARAAAFGEFLGKGIVYAKDTPNFVANRIG
                     VYAMMLTLHSMLDAGLNIEQVDKITGPPMGRPKSAAFGTADLVGIDTLAHVAKNCFDS
                     LPNDEERQVFALPDFVRELIEKGRLGRKSDGGFYKKVGADLLVYDVKTRDYRPQEKVR
                     FDSLGAIRNESDAGKRLKMLIAADDPAGRFAWKLLSRTLIYSARRVGEIADDVLNIDR
                     AMRWGFNWDMGPFEAWDAIGVAASVERMKAEGLAVPEWVSAMLASGRTRFYGEEPAKP
                     SYYDPRSKRLEAFPSDPREISLVALKSDPARVVKRNAGATLVDIGDGVLCVEFHTKLN
                     TLDGDVIGMLGEAVKLGEQSFDAIVIGNQGEHFCAGANLMLIAMAAQQKAWPQIEQIV
                     SAMQQSLLALRYSRIPVVAAPFQYTLGGGAEVAMAADARQAHAETYMGLVEVGVGLVP
                     AGGGCVRVLERYTAGLSALGADLLPFVGQASLNIATAKVATGAEEAKALRYLSATDGV
                     SLNASHHLYEAKQRALGLARAGYRPPLPPLLPAAGYDAARTISARIWGMAEGGYATAH
                     DALIANKVAFILCGGKVAAGTLLPEQHYLDLEREAFLSLCGEEKTMLRIQHMLMQGKP
                     LRN"
     gene            10522..10713
                     /locus_tag="VFQ35_03875"
     CDS             10522..10713
                     /locus_tag="VFQ35_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929795.1"
                     /translation="MRLRFGIGLALAGAASALFWALPHGGALAWTATLGLLASGAFAI
                     FEARKGWCAVRALGIKTPV"
     gene            complement(10697..>12676)
                     /locus_tag="VFQ35_03880"
     CDS             complement(10697..>12676)
                     /locus_tag="VFQ35_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9929796.1"
                     /translation="LDENGATRVRWLIDFTRPPAGLPGGAALETALDCDGARQCAVYS
                     KSDLSALLSDDVEGNEPRDEVGHGTHVASIATSNGRSSSTRQYAGVAPEADLVIARVT
                     TGESGITDPIILDATRFVFERAKELGEPAVVNLSLGSDLGAHDGSSALERALGSLVGP
                     SEPGRAIVVAAGNSGGLYRSVRKGFPDPFGIHTEVHVPRESTTRVSVITPVGSGISTL
                     GRVSVWIMSQPDDELSIGVARRGKLVGGMVPRGEAAQMTRGDLNFVIFNTNSATNPNT
                     AIVMIDGEWTSGEVFDLVFEGHGTASLWMEGAGDVDPAISIGPLFPLAQKEGTINVPA
                     ASPELIAVGATFNRADWIDIAGVAISRPSNGALQVSPPDTIAYFSAAGPNALGVIKPD
                     IVAPGSNVIAAMAAAADPRVNSHSSFAGTVTCGSAPLCLVVDDFHAVASGTSMAAPMV
                     SGVVALLLQQNPTLTQPELRALLQAGARRLEGAESFEPQVGPGAVDAHGALDALTVSA
                     AQTRLPGSASWISLATSYAHPDPTWPVRGYAELRDDDGHVADGFDPGRLLLVVRGGVV
                     SSPLQRILPGLYEFAFAAPNESGGTSLELSVRFDENTVAHRTVPIAVDRGALNGLAVP
                     RGGCGFATTNRGFDGAHWLLALVALLRRRRVKPAS"
BASE COUNT         2122 a         4175 c         4219 g         2160 t
ORIGIN      
        1 tggccacgac cggcgtcgcg cgctcggcgc gagcgaagcg cggcgcgagg tgcttgcgcg
       61 ccatgagcca gtcgtgaaga aaggcgccgc gatccccggc gatctcccgc gagcgcgacg
      121 ccggaaccac cagcgccacg ctgctcggcg acgtgggccg ggctaaagac ggccgacagc
      181 agaccgcggc tggggggctt acgctcggtc ttactgccgg ccgtcggaaa tcatcttaga
      241 gcgactttcg ctctccgtaa cgacaaaccg cagcatcgat gctcgaagca gcatcacccg
      301 cgaggttcac atatcgatgg cgaagcgctc tcgtgtccgg cggggtcgcc cgatgccgcg
      361 agggctcgca ggaacttacc cctggggatt gttgttctag aaggcgaggg cggcgtgagc
      421 cccgacgcgg aaccagtcga acgcgtcttc gtccttgacg aggaaggcgt cgtaagccgc
      481 gtggacaccc aaatcgatga agggcagcat cgtcaggtcg agcgtgacgc cggcgtcgat
      541 gctcgttccg ccccggctct cgtcgagaac tttcatgctc ccgtaaccga ggtgagcgaa
      601 aactccgggc tcgagcacct tgccaaaggt tgcgcgcccg ccgacgaagc cctgcaaaat
      661 tcggggatcc gcagcgccgc cgaaccagta accgccgagg cctacctcag gggttacgga
      721 cagcacgaga aggtcgagct tgtatccgta acgaagcgcg acgcctttgc cagcctcgga
      781 aaagtcctcg ttgatgtcgt tgatgtagtc gaggtcgact ccaagtcgcg atccgggagc
      841 ggcgtgagcg gtactgggcg cggcgacgaa gacagcgagc gctgcgaaca gcgctcccgc
      901 gcagcgcgca ccgaggcgtg aagagcagga cgacagcgaa ggagccaagc tcgtctgttt
      961 catggaaatt tccgtacgtg gcgaatggta ccggcagtgc cggcgagttc gagcaacaac
     1021 gggagacgtt gccgaagtct agccgccgag cgcgatcgag gcgtcgagca attgcaaaaa
     1081 gctgcgcggc tcgggcttgc tcgcaacgcc gctcaccgtc accacgtcca gggcgccgag
     1141 ggccgggcgt tggatgagct ggttgagctc tccggccgta ctcgcggccg gcatcgtcgc
     1201 gagcggtgga gcaccgacga cggagacttc cggaattgcg gacggcattt cgggcaacgg
     1261 cgccccatgc cgctcgcggt gctcttcgag ctcccgctcc gcctgagggg gcggcatcaa
     1321 cgcttcgtga tacgcgccgg cggactcctg tgcgggcaga gcggcctcga gatcgtccgg
     1381 atgtgactcg agcggtgcag cagagatcaa cggacggtcg agctctagct cgggccctcc
     1441 gctctcttcg agatcgatgg tttgaccgag ctgctcgacc gtcggccccg atgcaggcgg
     1501 aggcggacgg gtgccgaacg gctgctcgag cgaatcgaag ggagccgtga actgatccgc
     1561 tgccgcagcc ggcggcggac tgatctcttg atcgagcagg tgctcgacgt ccggagccgg
     1621 cggcgcgccg agtgaggcgg gcggcgaagc ttgcggcccc gactcgggcg gcggagtctt
     1681 gattgggact tcgcgagctt cgtcggcagc gagcgcaacg ccttcgtcga gctcatcgag
     1741 gtgcgcgacc ctgggccgct tcgaagacgc gggcggctct tcatcgatct cttcgaccac
     1801 cgcgccaggc gggatcgact cgagcatgtc ggacgggatc tcgagcagat cctcttcgcc
     1861 gagagcatcg gcggggccat tctcgaccac ctcgagctcg gcaacgtcgc ttacacgacg
     1921 cgcgaccatc acgtctgcga cctgctccgg agcctctggc cacccagact cgggagcctg
     1981 caccggaagc gagcgagctg ccggagccgg cgcctgccgc gaaaagggcg gcggaatggt
     2041 tactggatct tcggcgggcg gaaccggcgt gggcgccgcg tgcggagcgg gtgcgccggg
     2101 cgcgggcgga gccgccccga acgcgccacg tgcttctgct gcgcgtcgcc ggaccctcgt
     2161 gagcagagcc tcgaggcgtt gaagtcgcgt atcggtcacg ccgcacctgt tttgctgtcg
     2221 agctcggcga gtacctcgcc ccaggctcgg cccgattcga tcgaagattt cacgcgctcg
     2281 gcgccggcac ggctcgtcac gaggaaccga gcgcggtcgt cgtcgcgggc gagccgaacg
     2341 acgccgacgt cccgcccgaa ggcgcgctga atggctccct cgagagccgc ggccagcaag
     2401 tcgtcgccgc gcgcgccgaa gggcgaacca cgaaacgcca aaacgagcgc tcgtccccaa
     2461 cgctccagct cgagagagcc gagccccatg agcgcaagat cgccgccgag gtgctcgacc
     2521 accgactcgg ggttcaccga atccgtactg ccgaggcgct ctgcggtgcg ccgcccggct
     2581 tcggtcccga ggcgccgcgc gaaatccttg cgagcgtccg tatctaccgc gtcacatagc
     2641 gtgagcagtg cgtccgcggg gatgagtacg cgttcgccgc tgttcggaac ggcaatttgg
     2701 cctcgcgcga ggtcgaagcg cacggcggcc gtggggtcga agggaagcgt tggcatgagc
     2761 agacgactca gtccgaggag cgcagtatag gccgggcgcg gtgaatactg tcaaatctcg
     2821 ggagcgtgcc gcatcataga cccgtgccga gagccgcggc catcgccgct tgggtaaccg
     2881 ccgcgctggt ctcgatgacg gcgtccggcg cgatgcctag acgagaaccg ccgttgattc
     2941 tcggtggcgc gctgcctcga ccgaaggccg aggtcatcgt ggatccgcgg ctcccaacgg
     3001 gcgctcgggt aggtgccgca ccgagaccga ctgccaccgc gagcgcgtgt tcgtcgcggc
     3061 gcccggtatg tgtgcacgcc ttttcgcagc agggagctcg gctcgaggcg gcattggatg
     3121 cgctcgaacg cgcgtacgag cggctggtct acgcgctcgg gatgccggcg cccctttccg
     3181 atctggggct cggcggaacg gacgcgctcg acgcttacgt aggaggagag gcgctcctcg
     3241 tcgaggcgga tccgccgaag ttgggtccgt tcacagagag ctctggctgg tgcgagctcc
     3301 cgagcgtcga cggcgcgctc ttggatcgcg ccgcgactca gtgtgttggc gaggcaatcg
     3361 cgcttcgact cgacgcgagt gaaccgcctc acttgcgccg tgcgttcgcg acttggttgt
     3421 ggtggctggt gggcgcgccg acttcgctcg acctcgaggc aatcgatctg gttcaagcgc
     3481 accccgagcg ccccatcgcg agccgcgagc tctcggcaga gagcgaaggc gacgcgatgt
     3541 tcttcgagta cctcgagagc gcgcgcagca cacggccgtg gggagaactc tcggcggcgc
     3601 tcttcgggac cgccgtatcc gacaatcgca agctcgggct ctactacgac aacgaacccg
     3661 acttgttcga cgtgctgcgc cactcactca acgaggagcg cgatcgcttc gcggcgctga
     3721 tggtcgactt cgcggtggcg cgcgccttcg tgggttcgcg cgacgacggg ctgcatttgc
     3781 cgaacctcgc cttcagcggt gacttcgggc gcgtccgcta cgattggctc gtcaaattct
     3841 ccacgttgcc gcggcgggtg ctggtgaacc cgcccatcga ctcgactggc gccgtggtca
     3901 tttggctcga tctcgacgag gtggggctcg gggccgctct cggctttcac gccgattggg
     3961 agcctcccgt gagcttccat tggcagctcg tgaaggtcgg tccgaacggc gacgagatcg
     4021 ctcgcatcga cgtgccattt caagagcgcg ggcacgaggt cgacgcgcgc ctcacgaacc
     4081 tcgacggcgc aagcgcgatc ctcatcgtcg ggaccaacct cgaaggcatc gacctgtcgc
     4141 atcccttcga tcccgacgtg tcgccgttcg agccgcacgg cgcgaccgtg tacgtcgtgc
     4201 gcatgtgatt ctcagcgcac gtcgatggtc ctcaagctca ccatcacgag ttggttcccg
     4261 gagccggggt actttcccca aatgctgacg ccgtaacgtc cgcgcccggc accgagcggc
     4321 acttcgatcg agaagtgctt gccgtcgagc ttcacttctt tcggcgtctt gaagcccttc
     4381 gggaagtaca gaatgtacgg ctccggaatc gggtacgtgt tggttcggtt cagctcgcgc
     4441 gcgctgcgcg gcttgcgcgg ctcgatgcgc gacagcccga ccccgccgaa ctcgaccgga
     4501 tccttcacct cgccctcgac ggtgatcttg ctcccgaccc gcgcgctctt cggaaggtcg
     4561 tcgtagtctc ctcgctcgtc gacgaactct tgcgacatgc acgcgcccgg aacgccctcg
     4621 ggccgcgcga gaccgacccc gaagtgggtg tgcgtatctt tcaggatgtt gcgccggtgt
     4681 ccgtcggcag gagggcgctc ggccatgaac gcggcttcga ttttctcgag gtcctcggcc
     4741 gagaagctag ggttctgcac gagcgctcgc ttctggttgt cggcgaagca ccctgcgttt
     4801 tcttggacga agtcttctcc tcccgcgagc gtgtagcgct cttccggcac cgagccgtcg
     4861 gtgccccaat gcgccgtgta gctgaacgtg gccatgtcct cggcgtgcac ctggcccgcg
     4921 cgctcggcgg ccgcatcgtg ctcgacctcg gcgagcccgt tgtcgcgccg atcgcggttc
     4981 acgagggcga gcacgtattc gcgcgcctct tcgagtgaca tcggtcccgt ggggcgcgcg
     5041 agcaccgagg gcttcggtga acacgacgcc gccaacaccg agagcgcgag gagcacgcgg
     5101 ctgcgcggaa gcatccgtaa ttattctctg gcgccagagc gcccgccagg gctccgcgcc
     5161 cgcgacgcgc tcaggagtcc cgcgcgcgtc gatgtccgaa gaagctaccc ttcttgatga
     5221 cgtcgatgac ttcgggcgga acctgacgct cccagttggc gtcgccgctc cgaatcatgc
     5281 gcaagacgtc gttcgagaag atgttgaggc aatcgcggtc gaactcgtcg agctcgacga
     5341 tgcagccgcg atccaagagg tgaccgtaaa gccgcgcgag ctccgggttc ggcgtgaagt
     5401 tgctggcggt gacgagcttc tctcgcccgg ggtcgagcga tgggtatacg tagagcttcg
     5461 tgtcgttctt gaagagacga ccgaacgcct cgaggatgcc gccgtcgagg ttcgtgtagt
     5521 acttttcgtc gaacagcgag ctcaggctgc cggcgccgag caccaacgcg atgggccgct
     5581 gcgtgtaccg ccgcaaatac ccggcgagcc ggtagtactc gaagtaatcc gaaatcagca
     5641 ccgttttacc ggccgccgcc agcacgtccg cgcgcgccaa gaagtcgcga agatcgacgc
     5701 cgccctgacc gctctcgatc aggttgcgca tgttgatctc catgagctcg acgatgcgct
     5761 cgtcgcccga ggctccctct ttcacgaact tcttgcgtgc cgacgtcagc atgtcgatgt
     5821 gcacgcgcgt caccggcaaa aaggctccgc gctcgacgag cacggggctc ttccgcaaaa
     5881 gctccgaagg ctggagcacc tcacccttgg gatcgaaaat ggccgctccg gagagcccga
     5941 gctggacgag cttcagcgcc atcacgcggt tgtccacgtg ccggaactcg atgcccgaaa
     6001 actcgatcat gtcgatctcg atgcggtccg tgctcaggtt gtcgagcaac gattcgatca
     6061 agcgatcggg atcgtggtgc atgaagaacg cgccgtagag caggttcacc ccgacgacgc
     6121 cgagcgcctc ctgctgcagc gccatctcgt tgtcgagcaa gcgcacgtgg atgatgactt
     6181 ggctgtcttg atcgcggggg tgcgcctgga agcgcactcc gagccagccg tgacactcgt
     6241 tcgtaccctt gaagctcttc gcggcgaccg tgtccgcgaa aacgaagaac gacgtcgtgt
     6301 cgccgcgcgc gtcgcgaagg cgctcgagat tcaacttgtg ctcgtaggcg agcatgctct
     6361 cgaggcgctg acgacagacg taacgctgcg tgcgcccgta gatggcgtcg ctcaccgcca
     6421 tgtcgtaggc ggagatgctc tttgcaatgg ttcccgcggc gcctccgacg cggaagaacc
     6481 agcggacgac ttcttggcct gcgccgatct cggcgaaggt gccgtagcgc cgggcgtcga
     6541 gattcaccgc cagggctttt tgatgcgtgt cgagtttgtc cgtcgtccgg agaggtgaag
     6601 tcatggaagc gccgatagcc acgcgagcct taccttaatc gactgcgagg cgttctagcc
     6661 ctagcaaggt tgcccagcac acaccacgtt ggtccggctt cctagccgac gaagcttacc
     6721 ggaaacaatg cgcgaagggc cgggacttag agccagtcct tgaggccctg ctctgccgga
     6781 aggcgacggg agagattcag ggcccgcgtt tagccgccga cctcgaagag cccggccgcg
     6841 cccatgccac caccgataca catcgagacg attccgtaac gaccacctcg ccgcccgagt
     6901 tcgtgcagca gcgtcgcggt gagcttcgcg ccggtacacc ctagggggtg accgagcgct
     6961 atcgcgccgc cgttcacgtt gagcttctct ttcgggatgc cgagctcgcg ctggcagtag
     7021 acggcttgcg cggcgaaggc ctcgttgatt tcgaacagat cgatgtcttt gatgtcgagg
     7081 ccggtgagct tcaacacctt gcgaatcgcc gggatcgggc cgatgcccat gatgtcgggg
     7141 tccactcccg cgacggcgta agcgcgcagg cgaccgagcg ggcgtactcc caagtcggac
     7201 gcacgcttct cggacatcag caccgcggcc gcggcgccat cgctcagcgg cgagctgttg
     7261 ccggcagtca cgctgcccgc ctggctgaac gctggtttca gcttcgcgag cacgtcgagg
     7321 gtcgtgcccg cgcgcggcaa ttcgtcgacc gcgaaggtca tctccgcaac gccgttacct
     7381 tcgaagacgc gcgccggcac cggcacgatc tcctgggtga agcggcccgc cgtgatggct
     7441 tcgagggcgc gctcgtggga gagcacggca aaggcgtctt gatccgcccg cgaaaccgag
     7501 aaacgcgcgg cgacgcgctc ggcggtgtga cccatcgaga tgtacgcctc cgggtaggtg
     7561 tccatcaact tcgggttcag gctcaccttg ttgccgctca tcggcaccat cgacatcgac
     7621 tcgacgccgc cggcgagcac gacctccgcg agcccggttt ggatcgccat cgctccgtgc
     7681 acgagcgctt gcaggccgct cgagcaaaag cgattgatcg tctcggccgg aacgctgttc
     7741 ggaaagcgcg ccaagaacac cgcgttccgc gcgacgttga ggccttgctc gccttccggc
     7801 atcgcgcagc cgagcacgac gtcatcgagc gcagcggggt cgagcccttt cacgcgttct
     7861 accgcggcga ccatcgcgta cgccgcgaga tcgtcggggc gcgtgttgcg caaagttcct
     7921 ttgagagcgc gccccaccgc ggtgcgcgcg gccgaagcca caactacgtt gagcggaaat
     7981 cccatgtgcg tcctctcagt tgcggagggg cttgccctgc atgagcatgt gttgaatgcg
     8041 aagcatcgtc ttctcctcgc cgcagagcga caagaaggcc tcgcgctcga gatcgaggta
     8101 atgctgctcg gggagcagcg tacccgcggc gaccttgccg ccgcacagga tgaaggcgac
     8161 cttgttcgcg atgagcgcgt cgtgtgcggt ggcgtatccg ccctcggcca tgccccaaat
     8221 acgagccgaa atggtgcgcg cggcgtcgta accggcggcc ggcagcagcg gcggtaaagg
     8281 cggccgatag ccggcgcgag cgagcccgag agcgcgctgc ttggcttcgt acaagtgatg
     8341 cgaggcgttc agcgacacgc catcggtggc ggaaagataa cgtaaggcct tcgcctcctc
     8401 cgcgccagtc gccaccttcg cggtcgcgat gttgagcgac gcctgcccga cgaacggcaa
     8461 caggtcggcg ccgagcgcgc tgagcccggc cgtgtagcgc tcgaggacgc ggacgcagcc
     8521 gccgcccgcc ggcacgagcc cgactccgac ctcgacgagg cccatgtacg tctcggcgtg
     8581 ggcttgtcgc gcatcggccg ccatcgcgac ctccgcgccg ccgcccagcg tgtactggaa
     8641 cggcgccgca accaccggga ttcggctgta acgcagcgca agcagcgact gctgcatggc
     8701 gctcacgatt tgctcgattt gcggccacgc cttctgttga gccgccatcg cgatcaacat
     8761 caagttcgcg ccagcacaaa agtgctcgcc ctggttaccg atcacgatcg cgtcgaagct
     8821 ttgctcgccg agcttcaccg cttcgcccaa catgccgatg acgtcgccgt cgagggtgtt
     8881 gagcttcgtg tggaactcga cgcacaacac gccgtcgccg atgtcgacca acgtcgcgcc
     8941 ggcgtttctc ttcacgacgc gcgccgggtc gctcttcaac gcgaccaagc tgatctcgcg
     9001 cgggtcgctc gggaacgcct cgagccgctt gctgcgcgga tcgtagtagc tcggcttcgc
     9061 gggctcctcg ccgtaaaagc gagtgcgccc ggaggcgagc atggcgctca cccactctgg
     9121 cacggcgagg ccttcggcct tcatgcgttc gaccgaagcg gcgacgccaa tcgcgtccca
     9181 agcctcgaac ggacccatgt cccagttgaa gccccagcgc atggcgcggt cgatgttcag
     9241 gacgtcgtcc gcgatttcgc cgacgcggcg agcactgtag atcaacgtgc gcgagagcag
     9301 cttccaggcg aagcgccccg caggatcgtc cgccgcgatc agcatcttca gccgtttgcc
     9361 agcatccgac tcgttccgga tcgcgccgag cgaatcgaag cgcaccttct cctgcgggcg
     9421 gtagtcgcgc gttttgacgt cgtaaacgag cagatcggcg ccgacctttt tgtagaagcc
     9481 gccgtcgctc tttcgaccga ggcgtccttt ttcgatgagc tcgcgcacga agtcaggcag
     9541 cgcgaacacc tgccgctctt cgtcgttcgg cagcgagtcg aaacagttct tcgcgacgtg
     9601 tgccagcgta tcgatgccga cgagatcggc ggtgccaaaa gcggcgctct tcggtcgacc
     9661 catgggcggc cccgtgatct tgtcgacctg ttcgatgttg agccccgcgt cgagcatcga
     9721 gtgcagcgtc agcatcatcg cgtacacgcc gatgcggttc gcgacgaagt tgggcgtgtc
     9781 tttcgcgtag acgatgccct tccccaaaaa ctcgccgaac gccgctgcgc gcgcgagcac
     9841 cgtcggctcc gtgtgcggac ccggcgcgac ctcgagaagg cgcatgtagc gcaccggatt
     9901 gaagaaatgc aaaatcacga agcgcgattg gagcgcttcg gggagcacgt tcgagagcgc
     9961 cgcgacgctg agtccgctcg tattcgacgc caaaatcgcc tgctcgctca ggtacggctg
    10021 aatcgtggcg aagagcttct gcttcacgtc gaggttctcg accaccgctt cgaccacgag
    10081 atcgcactca cgcagccgct cgagatgatc ttcgaggttg cccgtctgaa ccaggcgcgc
    10141 cgcgtccgga tcgaagaaca aagcgggttt cgctttcagg gcacgctcga gcccgccctc
    10201 ggcgaagcga ttgcgcgcct tgggttgcgt gcgctcctct ggcttcaggt tgggaggcac
    10261 gatgtcgagc agatgcgtgc ggatgccggc gccggcgaaa tgcgcagcga tggcgctgcc
    10321 catcaccccg gcaccgatta ctcccgcggt tcggattcgt tcggcggcca tgttgacggg
    10381 atcgtggagc ggttcggcca cgaatgccag cgccaaagca gagtgggata tggtagccaa
    10441 agtcgcactt gacgctgagt ttgccggccc gaggtttgga cagtggctct cacgtgtaat
    10501 atcgacgccc gaggaaaagc ggtgcgactc cgctttggga ttggcctcgc gttggcgggc
    10561 gccgcgagcg cgctgttctg ggcgcttcct cacggaggcg cgctcgcctg gaccgcaacg
    10621 ctgggtttgc tcgcgagcgg agcgtttgcg atcttcgaag cgcgcaaagg ctggtgcgcc
    10681 gtgcgcgcgc tcggcatcaa gacgccggtt tgacgcgccg gcgccgcagc agcgcgacga
    10741 gggcgagcaa ccaatgggca ccgtcgaatc cgcggttcgt cgtcgcgaat ccgcaaccac
    10801 ctcgcggaac cgcgagaccg ttcagcgcgc cgcggtcgac cgcgatcggt accgttcggt
    10861 gcgcgaccgt gttttcgtcg aagcgcacgc tgagctcgag gctcgtcccg ccgctctcgt
    10921 tcggcgcggc gaacgcgaac tcatagagcc ccggcaggat ccgctgcagc ggtgagctga
    10981 cgactcctcc tcgaacgacg aggaggagcc gtcccgggtc gaagccgtca gcgacgtgac
    11041 cgtcgtcgtc ccgaagctct gcatagccgc ggacgggcca cgtggggtcg gggtgggcat
    11101 acgacgtcgc gagcgaaatc cagctcgcgg agccgggcag acgtgtttgc gccgcgctca
    11161 ccgtcaatgc atccagcgca ccatgcgcgt cgacggcgcc gggaccgacc tggggctcga
    11221 acgattctgc accttccagg cggcgcgcgc cggcttgcaa aagggcgcgc agctccggct
    11281 gagtgagcgt cggattttgt tggaggagca acgcgacgac accgctcacc atcggagctg
    11341 ccatcgacgt gccgctcgcg acggcgtgaa agtcgtcgac cacgaggcag agcggcgcgg
    11401 agccgcacgt cacggtgccg gcaaacgagc tgtgagagtt cacgcgcgga tccgccgcgg
    11461 ccgccatcgc cgcgatcacg ttcgagccgg gggcgacgat gtcgggcttg atgacgccga
    11521 gcgcgttcgg acccgcggcg ctgaagtagg cgatcgtatc cggcggcgag acctgcaacg
    11581 cgccgttcga agggcgcgaa atggcgacgc ctgcgatgtc gatccagtcg gctcgattga
    11641 aggtcgcccc gaccgcgatg agctccggcg aggctgcggg cacgttgatg gtgccttcct
    11701 tctgagccag tgggaacaag ggtccgatgc tgatcgcggg gtcgacgtcg ccggctcctt
    11761 ccatccagag cgaagcggtg ccgtgccctt cgaacaccaa atcgaagact tcgccgctcg
    11821 tccattcgcc gtcgatcatg acgatggcgg tgttggggtt cgtcgcgctg ttcgtgttga
    11881 aaatcacgaa atttagatcg ccgcgggtca tctgcgctgc ttcgccgcgc gggaccatgc
    11941 cgcccaccaa tttgcctcgg cgcgcgacgc caatcgacag ctcgtcatcg ggctgcgaca
    12001 tgatccaaac cgagacgcgg ccgagcgtgc tgatgccgct cccgaccgga gtgatgaccg
    12061 acacgcgcgt ggtcgattcg cgcggcacgt gcacttcggt gtgaataccg aacggatccg
    12121 gaaagccctt ccggacgctg cggtaaaggc ctccgctatt tccggcggcg accacgatcg
    12181 cgcggccggg ctcgctcgga ccgacgaggc tcccgagcgc tcgctcgagc gcagaagagc
    12241 cgtcgtgcgc gccgagatcg ctgcccagac tcaggttgac gacggctggc tcgccgagtt
    12301 ctttcgcgcg ttcgaacacg aagcgcgtcg cgtcgaggat gatcggatcc gtgatgccgc
    12361 tctccccagt ggtcacgcgc gcgatgacga gatcggcttc cggcgcgacg ccggcgtact
    12421 gccgcgtcga cgaagagcgc ccgttgctgg tcgcgatcga agcgacgtgc gtgccgtgcc
    12481 cgacctcgtc gcgcggctcg ttgccctcga cgtcgtcgct cagcaacgcc gagagatcac
    12541 tcttcgagta gacggcgcac tgacgtgctc cgtcacaatc gagcgcggtc tcgagcgctg
    12601 cgccgcccgg cagacccgcg ggcgggcgcg tgaaatcgat gagccagcgc acgcgtgtcg
    12661 ctccgttttc gtcgag
//
LOCUS       DASTKS010000184         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_250220, whole genome shotgun sequence.
ACCESSION   DASTKS010000184 DASTKS010000000
VERSION     DASTKS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250220"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="dnaX"
                     /locus_tag="VFQ35_03885"
     CDS             complement(<1..1126)
                     /gene="dnaX"
                     /locus_tag="VFQ35_03885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9929797.1"
                     /translation="MSYLVLARKWRPMRFEDLVGQDHVARTLANAIKTDRVAHAFLFT
                     GVRGVGKTTSARILAKALNCMGDPETRVAGVDPGPTVTPCLKCPACLEIAQGTDVDVR
                     EIDGASYNGVDEVRKLQDSIPYRPARDRFKIFIVDEVHMLSQSAWNAFLKTLEEPPPH
                     VKFIFATTEVHKVPVTILSRVQRFDFKLIPAQVVAARLRFVLAEEKIEADDAAVSIVA
                     REAAGSMRDAMSLLDQLIAWGGNQLRGDEVARVLGVASHAVLYEMARALVQGNAARCL
                     EVVADLSDMGQDIAHAARDLLALIRNLVVAKVSADPARLLDLPDSEAEDIKRLAKEAD
                     QDDLLRLHHGFTQGYDDVVKSGQPRAAFEMLLVRLARRPPL"
     gene            1383..2237
                     /locus_tag="VFQ35_03890"
     CDS             1383..2237
                     /locus_tag="VFQ35_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929798.1"
                     /translation="MLLKVRGVRPRERCAFVVAVASLSACAERGAATPTTPPLSNPAV
                     VASAKVPTASAAPVDTHGVSLEPLAAAALPAVLPHVEIVEPAFGDRLDVELARRTAIK
                     LRLENAPLSAEREGVLVALDGGRPRRFVETRPLTLGDLLPEDAELSTGAHTLLAVAVG
                     SDGRALRAEGAAKKPLSLVAFFIGVRPASAPAAAPASVFCLSPVGTFYTKPDEPVVLD
                     VLSFGLEKPLSTVRVRTRSFSFVAPFDSSRAFAIRGLPRGDVWLSVGENPRSECAVTL
                     NPPLEARP"
     gene            2243..3739
                     /locus_tag="VFQ35_03895"
     CDS             2243..3739
                     /locus_tag="VFQ35_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929799.1"
                     /translation="MFLVGVLSAWSVAHLGLALFFGLAYLLRPREREYLLFALLCTAF
                     SVLTASVAVEFSSSVVERQYSADQFTHAAAIMAAVFNVHFALSFARRGQPLPIMRWLY
                     AGAIAFVAAVFLELLWVPSSYRPRTAHVLGMAIAHGDGEVTPVGILFYVAGASATCAA
                     IWGFWQAYRSGRREVWAMLIASPLIAGATLHDVCLAAGAIDNSVVLLPHVFMLYALAA
                     GGTLLVRYRAAAGALEQTATNLKQRTEELRHSHAELVQVQNELVTKKQLAAVGELAAA
                     IAHEVRNPLAVIVNAVAGLRRPGLRPEDGTMLLDIVEEEAGRLNRLVTDLLRFARPVN
                     VKRTPVSLIELAKRCRTNIASGFQVEVQASDDPELQSVDVDPNLFRLVFDNLVANACQ
                     AMGDAGTVEIRVEKAELRGESAARVEIRDRGHGMDQHVLGRALDPFFTTRPSGTGLGL
                     PIVQRIVEAHGGELMLDSLEGEGTRVVLVVPMHASAEKGESAPRELMA"
     gene            3736..5025
                     /gene="bioA"
                     /locus_tag="VFQ35_03900"
     CDS             3736..5025
                     /gene="bioA"
                     /locus_tag="VFQ35_03900"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET9929800.1"
                     /translation="MTRRDRIVELDKRHVWHPYTPMQEYLNETNPLVIERASGARLFD
                     VDGRSYIDANSSWWSSVLGHDHPRLIAALNAQLSSLPHVALAGIAHEPAAELGAALAA
                     RAPGLPRVFFSDDGSTSIEVALKLSIQYWQQNGRPKRSRFIALEGAFHGDTLGATALG
                     GVEVFRRPFASVVLDCVHVTPEPSGYEAAFERLSQIVEREGDQIAAVVLEPMVQGAAG
                     MRIYDPALLRAAREVTRRYDTFLILDEVFSGFGRTGRFWAAEHAGVSPDLLCTSKGLS
                     GGLLPFAATLATERIFEGFLGGADRAFYYGHTFCGNPLGARVALEVLRVFDDEQILEV
                     AGQRARVIEQRFRRLAEVPGVLRTRSLGMIGALDLAGGDGYLQRRGQRVYLEALRRGA
                     YLRPLGNTVYVTPPLNISEADLSELLDVVEQSVIATN"
     gene            complement(5029..5379)
                     /locus_tag="VFQ35_03905"
     CDS             complement(5029..5379)
                     /locus_tag="VFQ35_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929801.1"
                     /translation="MTEKQPAESPCAPFASATTAEGLRVACYRVALDAIPVDFFADPD
                     GLWSFESLARAAGFRTDEGVAIGALRRPFGQHPDGAAVVTLNSEQRPYVAIIECPIAF
                     SCVDAQGASLAGAA"
BASE COUNT          825 a         1820 c         1816 g          992 t
ORIGIN      
        1 cgaggggagg gcggcgcgcg agccgcacga ggagcatttc gaaggcggcg cggggctgcc
       61 cgctcttcac gacgtcgtcg tagccttggg taaagccgtg gtgcagacgc aaaaggtcgt
      121 cttggtctgc ctctttggcg aggcgcttga tgtcttccgc ctcggagtcg ggcaaatcca
      181 agagccgcgc cggatcggcg ctcaccttgg cgaccactaa attccgaatc agcgccagca
      241 gatcgcgcgc ggcgtgcgcg atgtcttggc ccatgtccga gaggtcggcc acgacctcga
      301 ggcagcgcgc agcgttgcct tggacgagcg cgcgcgccat ctcgtacagc acggcgtggc
      361 ttgcgacgcc cagcacccga gcgacctcgt cgccgcgtag ctggttgccg ccccaggcga
      421 tcagctgatc gagcaggctc atggcgtcgc gcatgctgcc cgccgcctct cgcgcgacga
      481 tcgagaccgc ggcgtcgtct gcttcgattt tctcctcggc gaggacgaag cgcagccggg
      541 cggccacgac ctgggccggg atgagcttga aatcgaagcg ctggacgcgc gacaaaatcg
      601 tgacggggac cttgtggacc tcggtcgtcg cgaagatgaa cttcacgtgg ggcggcggct
      661 cctcgagcgt cttcaagaag gcgttccagg cgctctgcga gagcatgtgc acttcgtcga
      721 cgatgaagat cttgaagcga tctcgcgccg gtcggtaagg gatgctgtct tgcagcttcc
      781 tcacctcgtc gacgccgttg tagctcgcgc cgtcgatctc ccgaacgtcg acgtcggtgc
      841 cttgagcaat ttcgaggcac gccggacatt tcaggcacgg cgtgacggtc ggccccgggt
      901 cgacgccggc gacgcgcgtc tccggatcgc ccatgcagtt cagcgccttg gccaaaatgc
      961 gggcgctcgt ggtcttgccc acgcctcgca ccccagtgaa caaaaacgca tgcgcgacgc
     1021 gatcggtctt gatggcgttc gccagcgtgc gcgcgacgtg gtcctgacct accagatctt
     1081 cgaaccgcat cggccgccac ttgcgggcca ggaccaggta gctcattgca cggttcagct
     1141 agcagatcgg acccggtgcc gaaagtctca tcgggcgcgc cgccgagcca gcgaccgaac
     1201 tgcccctgcc gacactgatt tcgcggcttt cagcgccgcg acgggcgaat ggagccgtcg
     1261 ccaaaccgga gcctgtgcgc tagaacgccg attggtttga ctgctcggcg ttatagcctt
     1321 ggtttgcagg gacggcacgc gccgctcccg ttgactccgg gcccttaccg gctgactttg
     1381 ccatgctcct gaaagttcgc ggcgttcgcc cgcgtgagcg gtgcgcgttc gtggttgcgg
     1441 ttgcctcgct gagtgcgtgc gccgagcgag gcgcagcgac accgacgaca ccaccgctct
     1501 cgaaccctgc ggtcgtggcc tcggcgaagg tgccgaccgc gagtgcggcg cctgtcgaca
     1561 cgcatggtgt gagcctcgag cctctcgctg cggcggcgct gccggccgta ttaccccacg
     1621 tagagatcgt cgagcccgcg ttcggcgatc gactcgacgt cgagctcgcg cgtcgtaccg
     1681 cgatcaagct gcgactcgaa aatgctcctc tttcggcgga gcgcgagggt gtcctcgtgg
     1741 cgctcgacgg tgggcgtccg cgacgcttcg tcgaaactcg cccgctcacg ctcggcgatc
     1801 tcttgcccga agacgcggag ctttcgacag gagcgcacac gctgctcgcg gttgcggtgg
     1861 gctcggatgg gcgcgcgctg cgggccgaag gagctgcgaa gaagccgcta tcgctcgtcg
     1921 cgtttttcat cggggtgcgg cccgcgtctg cgcccgccgc ggcgccggcg agcgtctttt
     1981 gtctgagccc ggtcggcacc ttctacacga agccggacga gccggtcgtg ctcgatgtgc
     2041 tctcgttcgg cttggaaaag ccgctgtcga cggtccgcgt gaggacgcgg agcttctcgt
     2101 tcgtcgcgcc attcgactcg tcgcgcgctt tcgcgatacg cggtctgccg cgcggggacg
     2161 tgtggctttc cgtcggcgag aacccgcgct cggagtgcgc cgtgacgctc aaccctccgc
     2221 tcgaggcgcg gccgtgacgt cgatgttcct cgtcggagtg ctgagtgcgt ggtcggtggc
     2281 tcatctgggg ctcgcgttgt tcttcggcct cgcgtacctg ctgcgacccc gcgagcgtga
     2341 gtatctgctg ttcgcgctgc tttgcaccgc attcagcgtg ctcacggcca gcgtggcggt
     2401 cgagttctcg agctccgtcg tcgaacggca atattcggcg gatcaattca cgcacgccgc
     2461 cgccatcatg gccgcagtgt tcaacgtcca cttcgcgctg tcgttcgcgc gccgcgggca
     2521 gccgttgccg atcatgcgct ggctctacgc tggcgcgatt gcattcgtcg cggcggtgtt
     2581 cctcgagctc ttgtgggttc cgagctcgta tcgccctcgc accgcgcacg tgctcggcat
     2641 ggcgatcgcc cacggcgatg gcgaggtcac gccggtcggc atcttgttct acgtggcggg
     2701 cgcgagcgct acttgcgccg ctatttgggg gttctggcag gcatacagga gcgggcggcg
     2761 cgaagtctgg gccatgctca tcgcgtcacc cctcatcgcg ggggcgacgc tccacgacgt
     2821 ctgcctggcc gccggcgcca tcgacaacag cgtcgttctc ctgccgcacg tcttcatgct
     2881 gtacgcgctc gccgccggcg gaacactgct agttcgatat cgggcggccg cgggcgcgct
     2941 cgagcagacc gcgacgaacc tgaagcagcg caccgaggag ctgaggcact cgcatgccga
     3001 gctcgtgcag gttcaaaacg aactcgtcac caagaagcag ctcgcggccg tcggtgagct
     3061 cgccgcggcg atcgcgcacg aagtccgcaa cccgctcgcg gtcatcgtca acgcggtggc
     3121 cggcttgcgc cgccccggct tgcgcccgga agacggcacg atgttgctcg acatcgtcga
     3181 agaagaggcc gggcgcttga atcgcctcgt gacggatctg ttgcgcttcg cgcggccggt
     3241 caacgtcaag cgcactcccg tctcgctgat cgagctcgcc aagcgttgcc gcacgaacat
     3301 cgcgtcgggt tttcaagtcg aggtacaagc gtcggacgat cccgagctgc agagcgtcga
     3361 cgtcgatccg aacttgtttc gcctcgtctt cgacaacttg gtcgcgaacg cctgccaagc
     3421 catgggcgac gctggcaccg tggagatccg cgtcgaaaaa gcggagcttc gcggtgaatc
     3481 ggctgcgcgc gtcgaaattc gggaccgcgg ccacgggatg gatcagcacg tcctcggccg
     3541 cgcgctcgac cccttcttca ccacgcggcc gagcgggacg gggctcgggc tacccatcgt
     3601 gcaacgcatc gtggaagcac acggaggaga gttgatgttg gacagtttgg agggtgaggg
     3661 gactcgcgta gtcctcgtcg ttccgatgca cgcttcggca gaaaagggcg aatcggcacc
     3721 gcgggagctc atggcatgac acggcgcgat cgcatcgtcg aactcgacaa gcggcacgtg
     3781 tggcacccct acactccaat gcaggagtac ctcaacgaga ccaacccgct cgtgatcgaa
     3841 cgcgcgagcg gcgcgcggct cttcgacgtc gacggtcgca gctacatcga cgccaactcg
     3901 tcgtggtgga gctctgtgct cgggcacgac catccacggc tcatcgccgc gctcaacgcg
     3961 cagctttcga gcttgcctca cgtcgcgctg gccggcatcg cccacgagcc agcggccgag
     4021 ctcggcgccg ccctcgccgc gcgcgcgccc gggctcccgc gcgtcttctt cagcgacgac
     4081 ggctcgactt cgatcgaagt ggcgctgaag ctctcgattc aatactggca gcagaatggc
     4141 cgcccgaagc gctcgcgctt catcgcgctc gaaggcgcgt tccacggtga cacgctgggg
     4201 gccaccgcgc tcggcggcgt cgaagtgttt cgtcgcccgt tcgcgtcggt cgtgctcgac
     4261 tgcgttcacg tcacgccgga gccgagcggg tacgaagctg cattcgagcg cctcagccaa
     4321 atcgtcgagc gagagggcga ccagatcgcg gcggtggtgc tcgagccgat ggttcaaggc
     4381 gccgcgggca tgcgcatcta cgatcccgcg ctgctccgcg cggcgcgcga ggtgacgcgt
     4441 cgctacgata ccttcttgat cttggacgag gtgttcagcg ggttcggccg aaccggacgc
     4501 ttctgggcgg ccgagcacgc cggtgtgtcg cccgatctgc tctgcacctc gaagggcttg
     4561 agcggtggcc tgctgccgtt cgccgccacg ctcgcgacgg agcgcatttt cgagggattt
     4621 ctcggaggtg cggaccgcgc cttctattac ggtcacacct tctgtggcaa tccgctcggg
     4681 gcccgcgtcg ccctcgaagt gctgcgcgtc ttcgatgacg agcagattct ggaggttgcc
     4741 ggtcaacgcg ctcgcgtgat cgagcagcgg tttcgtcgct tggccgaagt gccaggcgtc
     4801 cttcgcacgc gctcgctcgg gatgattggc gcgctcgacc tcgcgggcgg tgacggctac
     4861 ctgcaacgac gcgggcagcg cgtttacctc gaagcgttgc gccgcggtgc ctacctgcgt
     4921 ccgctcggca acaccgtgta cgtcactccg ccgctcaaca tcagcgaggc ggatctttcc
     4981 gagctactcg atgtcgtcga gcagtcggtc atcgcaacca actgatgctc acgcggcccc
     5041 ggccaggctc gccccctgcg cgtcgacgca gctgaatgcg atggggcact cgatgatggc
     5101 gacgtacggc cgctgctcac tattcagggt gaccacggct gcgccatcgg gatgctggcc
     5161 aaacggtcgc cgcagcgcgc cgatggcgac cccttcgtcc gtgcgaaagc ccgccgccct
     5221 ggccaacgac tcgaagctcc agaggccgtc tggatctgca aaaaagtcga ctggaattgc
     5281 gtcgagcgcg acgcggtaac acgcgacgcg cagtccttcg gcggtggtcg cgctcgcgaa
     5341 aggcgcgcaa ggcgactctg ctggctgttt ttccgtcatg ggacgtctcg ctacgtaatg
     5401 ggcttgcggg ccttcgatga gttcgaccgc ggtgcgcaag actcagttcc gac
//
LOCUS       DASTKS010000185         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_697514, whole genome shotgun sequence.
ACCESSION   DASTKS010000185 DASTKS010000000
VERSION     DASTKS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697514"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFQ35_03910"
     CDS             <1..466
                     /locus_tag="VFQ35_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9929802.1"
                     /translation="LFETEWTLLLRDSGGFGGPRPPKSDAPKPGAGQAPSFSITLPTS
                     PEQALLYRLSGDLNPLHADPDFAARAGFPEGPILHGLCTFGVMTRALIKHLGGDERKI
                     RALGAQFRKPVWPGDPIRTEGYALDGGRFALEAFAADRPDAVISGAWAEVSS"
     gene            479..1297
                     /locus_tag="VFQ35_03915"
     CDS             479..1297
                     /locus_tag="VFQ35_03915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9929803.1"
                     /translation="MSDDKTNKVQSVPPPATDDIDHEWGGTPAPPGVAASKAAKTPDA
                     AVSAAAPHQPVKGRDEEEEEEDEEEEEDGEDEEEEEEEERHGHAASARPVSTSSHRPK
                     SGDDWLPDWAPWAVLGGLVIIGLVGGLGGLTKPSAERVARVQAESEQAHAAAQPAAAQ
                     VAAAPETIEASHLLVAYQGAMRANQTITRTKEQAKQRAEEAAAKAKKGVPFDKLVAEY
                     SDEPGAAARGGKLGSFSRQQMVKPFADAAFALKPNAISGVVETQFGFHVIQRTK"
     gene            1294..1767
                     /locus_tag="VFQ35_03920"
     CDS             1294..1767
                     /locus_tag="VFQ35_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9929804.1"
                     /translation="MKRLLPLLLLSSGCVVTTLEGPGDPPRVAPPPGRAQELKAANEA
                     PIPTGPETIGARHLLVSYRGAMRASPTVTRTKEEARARVAEALEKARGGAEFEDLVKE
                     YSDEPGAAERGGDLGKFKRTMMVKPFADAAFKLAPGELSDVVETPFGFHVIQRTE"
     gene            complement(1769..2914)
                     /locus_tag="VFQ35_03925"
     CDS             complement(1769..2914)
                     /locus_tag="VFQ35_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9929805.1"
                     /translation="MDFELPESHKLLRETVRDFALREVKPRARDWDREERFPIEMMPK
                     LAELGLLGIKIPEAYGGAGLDMLSYALCVEEIARVDGSLALTVASHNGLGTGHILAVG
                     TEEQKRKYLTRAAAGEWLAAWALTEPGSGSDSAAMKTTARRDGDHWVLRGSKMFITQG
                     SVGGFCVVLARTRFDVPAQRGITAFIVETSTPGYSASKKLEKYGCRSSDTVEITLDDV
                     RVPDSERLGEVDAGFRDTMRILDGGRVSIAAMALGLGRGALEMAVNYAKERQAFGRPI
                     AEFQALQFMLADAKTELEAAALMTYRSAWLADQGRRFTEEASMAKLFASEVATRACNA
                     ALQIHGGYGYTREFDVERHLRDVKLCEIGEGTSQVQRMVIAKNLLRE"
     gene            complement(3010..>3291)
                     /locus_tag="VFQ35_03930"
     CDS             complement(3010..>3291)
                     /locus_tag="VFQ35_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929806.1"
                     /translation="TLKKVEGAKSIKDSPIDAVIVNLHESEGAPKVEWLVGRSGALVA
                     GVGDEAFVAAKSGGAKATFSDQDRLTLLRSVLSGSKSPASPAASGSAAP"
BASE COUNT          572 a         1122 c         1055 g          542 t
ORIGIN      
        1 gcttttcgag acggaatgga cgctgctcct gcgcgactcg ggtggcttcg gaggtccgcg
       61 cccgccaaag agcgacgcgc cgaagccggg tgcggggcaa gctccgagct tttcgatcac
      121 gctgcccacc tcgccagagc aggcgctttt gtatcggctt tcgggcgatc tgaatccgtt
      181 gcatgcggat cccgatttcg ccgcgcgtgc tggttttccc gaagggccca ttttgcacgg
      241 gctttgcact ttcggggtca tgactcgcgc gctcatcaag cacctcggcg gcgatgagcg
      301 aaagattcgt gctctcgggg cgcagttcag aaaacccgta tggccaggtg accccatccg
      361 gacggagggc tacgctctcg acggaggtcg ctttgcgctg gaggccttcg ccgccgaccg
      421 tcccgacgcc gtcatcagcg gcgcatgggc cgaggtaagt tcctgaggag tgtcagcgat
      481 gtccgacgat aaaaccaaca aggtccaatc cgttcccccg ccggccaccg acgacatcga
      541 tcacgagtgg ggcggcaccc ctgccccgcc gggagttgcc gcttccaaag ccgcaaaaac
      601 tccggacgcc gccgtctctg ccgccgcgcc tcatcaaccc gtgaagggtc gagacgaaga
      661 ggaagaggaa gaagacgaag aagaagaaga ggacggggaa gacgaagaag aggaagaaga
      721 agaagagcgg cacgggcacg ccgcgtcggc gcgacccgtt tcgacctcga gtcaccgtcc
      781 gaagagcggc gacgattggc ttcccgattg ggcgccgtgg gcggtcttgg gaggcctcgt
      841 gatcatcggc ctcgttggcg ggctcggtgg gttgaccaag ccgagcgccg aacgcgtcgc
      901 tcgcgtgcag gccgagtccg agcaggctca cgccgccgcc cagcccgcgg ctgctcaggt
      961 ggctgccgct cccgagacga tcgaggcgag tcaccttctc gttgcgtacc aaggagcgat
     1021 gcgcgcgaac caaaccatca cgcgcacgaa ggagcaggcc aagcagcgag ccgaagaagc
     1081 cgccgccaag gccaagaagg gggtcccctt cgataaactc gtcgctgagt actccgacga
     1141 gccaggagcc gcagctcgcg ggggcaagct cggttcgttc agtcgccagc aaatggtaaa
     1201 gccgttcgcc gatgcggcct tcgccttgaa gccgaacgcg atttccggag tcgtcgagac
     1261 tcagttcggc tttcacgtca tccagcggac caagtgaaac gcctcctccc cctgttgctg
     1321 ttgtcgtccg ggtgcgtcgt caccacgctc gaaggaccgg gtgacccgcc gcgggtcgct
     1381 cccccacccg gacgcgctca agagctcaaa gccgcgaacg aagcgccgat cccgaccggc
     1441 cccgagacga tcggagcgcg ccacctcttg gtctcttatc ggggcgcgat gcgcgcgagc
     1501 ccgaccgtca cccgaaccaa ggaagaagcg cgcgcccgcg tggcggaggc gctcgaaaaa
     1561 gcacgcggcg gcgcagagtt cgaagatctc gtgaaggagt attcggacga gcccggcgcg
     1621 gccgagcgcg gcggcgatct cggaaagttc aagcgcacga tgatggtgaa gccattcgcc
     1681 gacgctgcct tcaagctcgc ccccggcgag ctatccgacg tggtcgagac gcccttcggc
     1741 ttccacgtca tccagcgcac cgagtagctc actcgcgcag caggttcttg gcgatgacca
     1801 tgcgctggac ctggctcgtc ccctcgccaa tttcgcagag ctttacgtcg cgcaggtgtc
     1861 gctcgacgtc gaactcgcgc gtgtagccat agccgccgtg gatttgcagc gcggcgttgc
     1921 aagcgcgggt cgcgacttcg ctcgcgaaga gcttcgccat cgaggcttcc tccgtgaatc
     1981 gccgcccctg gtcagcgagc cacgcggagc gatacgtcat caacgcggcc gcctcgagct
     2041 ccgtcttcgc gtccgcgagc atgaactgca gcgcttggaa ctcggcgatg ggtcgaccga
     2101 acgcctggcg ctccttcgcg tagttcacgg ccatttcgag cgcgccgcgg ccgaggccga
     2161 gggccatcgc ggcgatcgaa acgcgccccc cgtcgaggat acgcatcgtg tcgcgaaagc
     2221 ccgcatcgac ttccccgagg cgctcgctgt ccggcactcg cacgtcgtcg agcgtgatct
     2281 cgaccgtatc gctcgaacgg cagccgtact tctcgagctt cttgctcgcc gaatagcccg
     2341 gcgtggaagt ctcgacgatg aacgcagtga tgcctcgttg cgcgggtacg tcgaagcgcg
     2401 tgcgagcgag caccacgcag aacccaccca ccgagccctg ggtgatgaac atcttcgagc
     2461 cgcgcagcac ccagtggtcg ccgtcgcggc gcgcagtggt cttcatcgcg gcgctgtccg
     2521 accccgagcc aggctcggtc agcgcccagg ccgcgagcca ctcacccgcc gcggcccggg
     2581 tcagatactt gcgcttctgc tcctcggtcc cgaccgcgag gatgtgcccc gtgccgagcc
     2641 cgttgtgact cgcgaccgtg agcgcgagcg agccgtcgac gcgcgcgatt tcctcgacgc
     2701 agagcgcata cgagagcatg tcgaggccgg ctccgccgta cgcctctggg atcttgatgc
     2761 cgagaaggcc aagctccgcg agcttcggca tcatctcgat cgggaagcgt tcttcgcggt
     2821 cccagtcgcg tgcgcgcggc ttcacctcgc gcaacgcaaa gtccctcacc gtttcccgca
     2881 acaacttgtg tgactcaggc agctcgaaat ccaatcggca cgctcccgtc tcgcatcagg
     2941 tgggtgcttc cattgtgacc cgcgctgccg aggccggtcg agccctcgac tgtgggagaa
     3001 tgtacgcgtt cagggagcag cgctgcccga ggcggccggg ctcgccgggg atttgctccc
     3061 gctgaggacg ctccggagca gcgtgaggcg atcctggtcc gagaaggtcg ccttggcgcc
     3121 gccgctcttc gcggccacga aagcttcgtc ccctacgccg gcgaccagcg ctccgctccg
     3181 cccgaccagc cactcgacct tgggcgcccc ctcgctctcg tgcaggttca cgatcacggc
     3241 atcgatggga gaatctttga tcgatttggc gccctcgacc tttttcaagg t
//
LOCUS       DASTKS010000186         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_920129, whole genome shotgun sequence.
ACCESSION   DASTKS010000186 DASTKS010000000
VERSION     DASTKS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920129"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1664
                     /locus_tag="VFQ35_03935"
     CDS             51..1664
                     /locus_tag="VFQ35_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9929807.1"
                     /translation="MQLELYVQSPNDTFVHALDLEGRSITLGRDEGCSVRLNDEHVSR
                     RHVTLWLENGKLRVQDSSRNGTWAGKAFLRNEDTAVPFGTPIRLGQTVISLSPARPTT
                     EPPPPPPSRDGDPSSVPVELRREAHRLLLEHLDLGSGDLLGMDDVSLRPRVLQSLRRI
                     VTSLASRLPEGVDIDRLVGELADEALGLGPLEELLADPSVSEIMVVDPQTVYVERRGR
                     CERASQRFTDDDRVRAVVERIVTPLGRRIDESSPLVDARLRDGSRVNAIIRPLAIRGT
                     CITIRKFPKYQLTLEKMIDLGSIDERVVRFLQRSVRARKNIVVAGGTGSGKTTLLNAL
                     SAAIPEHERVVTVEDSAELQLTQPHVVSLETRPANMEGKGAYTIRDLVKNALRMRPDR
                     IVVGECRGGEALDMLQAMNTGHDGSLTTVHANSPREAVGRLETLCLMAGLELPVKAIR
                     EQIASSVNLFVQQSRYQDGTRRVSAVSEVCGIDSKGMIVQRPIYEFVQTGVDESGRVV
                     GEFLATGYLPSYLNEFIVRGMIPEGEPYL"
     gene            1661..2527
                     /locus_tag="VFQ35_03940"
     CDS             1661..2527
                     /locus_tag="VFQ35_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9929808.1"
                     /translation="MTHDILALKYGCLLSASLAAGVAAVSLARAPDSAVRVHWSRYVQ
                     KLDESFEVLRLPFSGARIARLQALVLLALTAFTATFRWEVKQAIEFAAFTLFAPMLLV
                     SHALRKRFARIERDTTGLLVGLANSLKASPSLGKALGRVETILSGPLATEIAEVTRDI
                     RLGASVDQALLALGARVKSPSLDSALTGILIGRQVGGNLPEILETTASTLREMERLDG
                     VVRSKTSEGKAQVVVIALAPPIVFLAFDNLEHGYFDPFTTSFAGMGLLALAGTMWIAA
                     VIMAKHILTVDI"
BASE COUNT          421 a          851 c          855 g          435 t
ORIGIN      
        1 cgggcagcgt gcagggagat ccgcacggcc acccaggagc acgctgactc atgcaactgg
       61 aactctacgt tcagtcgccg aacgacacgt tcgtgcacgc gctcgacctc gaggggcgca
      121 gcatcacgct cgggcgagac gaaggttgct cggttcgctt gaacgacgag cacgtctcgc
      181 gccgccacgt cacgctctgg ctcgaaaacg gcaagctccg cgtgcaggat tcgtcgagga
      241 acggcacctg ggcgggcaag gctttccttc gcaacgaaga caccgcggtt ccgttcggca
      301 cgccgattcg cctcggtcag acggtgattt cgctctcacc ggcgcggccg acgactgagc
      361 cgccgcctcc accaccatca cgcgacgggg acccgagcag cgtgccagtg gagcttcgcc
      421 gcgaggctca ccgcttgttg ctcgagcacc tcgatctcgg ttcgggcgac ctgttgggca
      481 tggacgacgt gtcgctgcgg ccgcgcgtgc tgcagagctt gcgacgcatc gtgacgagcc
      541 tcgcgtcgcg gctgccggag ggcgtggaca tcgatcgcct ggtcggagaa ctcgcggacg
      601 aggcgcttgg gctcggaccc ctcgaggagc tgctcgcgga tccgagcgtc tccgaaatca
      661 tggtggtgga cccgcagact gtctatgtcg aacgacgcgg gcgctgcgag cgggcgtcgc
      721 aacgatttac ggacgatgac cgcgtgcgcg cggtggtcga gcgcatcgtg acgccgctcg
      781 gccgccgcat cgacgagtcg agcccgctcg tcgacgcgcg cttgagggac ggctcgcgcg
      841 tgaacgccat cattcggccg ctcgcgattc ggggcacctg catcacgatc cgcaagttcc
      901 cgaagtatca gctcacgctc gagaagatga tcgacctcgg tagcatcgac gagcgcgtcg
      961 tcaggttttt gcagcgcagc gtcagggcgc gcaagaacat cgtcgtcgct ggcggcaccg
     1021 gcagcggcaa gacgacgctg ctgaacgcgc tctcggccgc gatccccgag catgaacgcg
     1081 tcgtcacggt cgaagactcc gccgagctcc agctcacgca accgcacgtg gtttcgctcg
     1141 agacgcgccc cgccaacatg gagggtaaag gcgcctacac gatccgcgat ctcgtcaaaa
     1201 atgccctccg catgcgcccg gatcgcatcg tggtgggcga gtgccgcggc ggcgaggcgc
     1261 tcgacatgtt gcaggccatg aacacgggtc acgacggttc gctcacgacc gtccacgcga
     1321 actcgccgcg cgaagccgtg gggcgcctgg agacgctctg cttgatggcg gggctcgagc
     1381 taccggtcaa ggctattcgc gagcaaatcg cgagcagcgt caacctgttc gtccagcaga
     1441 gccgctatca agacggcacg cgccgggtga gcgccgtgtc ggaggtgtgt gggatcgact
     1501 cgaagggcat gatcgtgcag cgcccgattt acgagttcgt gcagaccggt gtcgacgaga
     1561 gcgggcgcgt cgtgggcgag ttcctcgcga cgggttacct gccgagttac ctcaacgagt
     1621 tcatcgtgcg gggcatgatc cccgaggggg agccgtacct gtgacccacg atatcctcgc
     1681 cctcaagtac ggctgcttgc tttcggcgtc gctcgcggcc ggcgtcgccg cggtgtcgct
     1741 ggcgcgtgcg ccggacagcg cggtgcgcgt gcactggtcc cgctacgtgc agaagctcga
     1801 cgaaagcttc gaggttctgc ggctgccctt ctccggcgct cgcatcgcgc ggctgcaagc
     1861 gctggtccta ctcgccctca ccgcgttcac ggcgacgttt cgttgggagg tgaagcaagc
     1921 catcgagttt gccgccttca cgctgtttgc gccgatgctg ctcgtgtcgc acgcgctgcg
     1981 caaacgcttc gcgcgcatcg agcgcgacac caccggtcta ctcgtcgggc tcgcgaacag
     2041 cttgaaggcg agccccagcc tcggaaaggc gctcgggcgc gtcgaaacca tcctgagcgg
     2101 cccgctcgcc accgaaatcg cggaggtgac gcgcgacatt cggctcggcg cgagcgtcga
     2161 tcaggcgttg ctcgctctcg gggcgcgcgt aaagagcccc tccctcgact ccgcgctgac
     2221 cggcatcctc atcggacgtc aggtcggcgg aaaccttccg gagatcctcg agacgaccgc
     2281 gagcacgctg cgcgagatgg agcgcctcga cggcgtcgtg cgctcgaaga cgtcggaggg
     2341 caaagctcag gtcgtcgtca tcgcgctcgc tccgccgatc gtgttcctcg ccttcgacaa
     2401 cttggagcac ggctacttcg acccgttcac gactagcttt gcagggatgg ggctcttggc
     2461 gctcgcgggc acgatgtgga tcgcggccgt gatcatggcg aagcacattc tcacggtgga
     2521 catctgatgg cgtcgctcga atcgctcaag ctcgccttcg tg
//
LOCUS       DASTKS010000187         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_29427,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000187 DASTKS010000000
VERSION     DASTKS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29427"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFQ35_03945"
     CDS             <1..429
                     /locus_tag="VFQ35_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9929809.1"
                     /translation="ILHRDLKPRNIFVLERGGVRLLDFGFAKFTRMRTVTQAGLIAGS
                     PNYIAPEIWRGALHFDHRIDVYSFGALIFRALSGHMPFVAEGMKEILRAVTTAERPSL
                     QALRPDLPAAIDDWVQLALAIDPNHRFERVQAMWNALSSL"
     gene            complement(431..874)
                     /gene="dut"
                     /locus_tag="VFQ35_03950"
     CDS             complement(431..874)
                     /gene="dut"
                     /locus_tag="VFQ35_03950"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9929810.1"
                     /translation="MKVTISVQRTGAVRVNLPSYRTAGSAGLDLEAALEAAVVLAPGE
                     RRLIPTGLCMEIPAGFEGQVRPRSGLALKHGIGMVNAPGTIDSDYRGEIGVLLINHGH
                     EPFAITPGARIAQLVICPVAHAELVEVERLGGTERAAGGYGSTGT"
     gene            complement(891..>2917)
                     /locus_tag="VFQ35_03955"
     CDS             complement(891..>2917)
                     /locus_tag="VFQ35_03955"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929811.1"
                     /translation="RGKLLVVCNTRPEFLSRHTEWAALAGSRHTLLEVGPVAEDDAIA
                     IMQSLLARCQGGPPPRLVEAAVSMAGGAPGLLEHMVSVFRDAGVLLEDPNDVGGGWRV
                     DLDRLASVRLPLTVEDAVQVRIAALAPHERRLLEHAATMGSVFWLSALVALGRIDREP
                     PELWAESGTDDVSAVENALSELTARDYVLKVPDSAFPGEVEYVFKHNLERETIARLTA
                     APSARRYHQTIADWLAQKDNVRNQEEYSAMLARHLEHAGSLTRAAFTYIESGNTAREH
                     FAAKRADEYYRKGLELLGEDDARRRIDALHNHGDVLVLLGRTDEALAAFREMLGIAYR
                     LGLRSKGGAAHNRIGRLYRDTGSLGPAQRHFETALALFESSGDQRGVASCRDDIGRLL
                     WMRGEYQEALDAMRNALEMRKSHGDGRSIALSLNNIGLVWRDHGKVNEASEALESALK
                     IRRELNDPLGIVESLNDLGELALDQNDPNQALQLFQEARDVATDIGERNRIAVVLTNM
                     GAAYLRLGDADRSVAVLTQAEELCDELGDKLHLAEAKRGLAKAYLAKGELKRARDSIK
                     RSVDLFGQVRSKPHLAMALRTLGEVTAAGAWGDGHEGRAVDYFLRSIAICQEIGNEVE
                     VAKSYRAFSSYVEGSPHYRHNADIVREAKKLSHMADEIFKRHKLGTLSRP"
BASE COUNT          442 a          964 c          983 g          528 t
ORIGIN      
        1 attttgcacc gtgacctgaa gccgaggaac attttcgtct tggaacgagg cggcgtgcgt
       61 ctcttggatt tcgggttcgc gaagttcacg cgaatgcgca cggtgacgca ggcggggctc
      121 atcgccggtt cgccgaacta catcgccccg gagatatggc gcggcgcgct ccatttcgat
      181 caccgcatcg acgtctactc gttcggcgcg ctcatttttc gcgcgttgag cgggcacatg
      241 ccgttcgtcg ccgaaggcat gaaggagatc ttgcgcgccg tgacgacggc agagaggccg
      301 agcctgcaag ccctgcggcc cgatttgccg gccgccatcg acgactgggt gcagctcgcc
      361 ctcgccatcg atccgaacca ccgcttcgag cgagttcagg cgatgtggaa cgccctgagc
      421 tcgctctgat tcaggtaccg gtcgagccgt aaccgccagc ggcgcgctcg gtgccgccca
      481 ggcgctcgac ttcgacgagc tccgcgtgcg ccaccgggca aatcacgagc tgggcgatgc
      541 gcgcccccgg cgtgatcgcg aacggctcgt ggccgtgatt gatgagcaaa accccaattt
      601 caccgcggta gtcgctgtcg atcgttcccg gtgcgttcac catcccgatc ccatgcttca
      661 acgcgagccc ggagcgcggc cgaacttggc cttcgaaccc cgcggggatt tccatacaaa
      721 ggcccgtcgg gatcaagcgc cgctcacccg gtgcgagcac gaccgcggcc tcgagcgcgg
      781 cctcgagatc gagccccgct gagcccgcgg tgcgatagct cgggaggttg acgcgcacgg
      841 cgccggtgcg ttgaacggaa atggtgactt tcatgtgagg cgttcctcgt tcacgggcgg
      901 ctcagcgtgc cgagcttgtg gcgtttgaag atctcgtcgg ccatgtgcga gagcttcttc
      961 gcctcgcgga cgatatctgc gttgtggcga tagtgtggag agccctcgac gtagctcgag
     1021 aacgcgcggt aactcttcgc gacctcgacc tcattgccga tctcctggca tatcgcgatc
     1081 gaccgcagga aatagtcgac ggcgcggccc tcgtgtccgt cgccccaagc gccggcggcg
     1141 gttacttcgc cgagcgtccg gagggccatc gcgaggtgcg gcttgctcct cacttgaccg
     1201 aagaggtcga cgctccgctt gatcgagtca cgcgcacgct tgagctcgcc cttcgcgagg
     1261 tacgccttgg cgaggccgcg cttggcttcg gcgaggtgca gcttgtctcc gagctcgtcg
     1321 cagagctcct cggcttgagt gagcacggcg accgagcgat ctgcgtcgcc gaggcgaaga
     1381 taggccgcgc ccatgttcgt gagcacgacg gcgatgcggt tgcgctcgcc gatgtcggtc
     1441 gcgacgtcgc gcgcttcctg aaatagctgc agcgcctgat tgggatcgtt ctggtcgagg
     1501 gcgagctcac cgagatcgtt caagctctcg acgatgccga gcgggtcgtt gagctcgcgg
     1561 cggatcttga gggcgctctc gagcgcttcg ctcgcctcgt tgaccttgcc gtgatcgcgc
     1621 cacacgaggc cgatgttgtt caagctgaga gcgatggagc gaccatcccc gtgcgatttg
     1681 cgcatttcca gcgcattgcg catggcgtcg agggcctctt ggtactcgcc gcgcatccag
     1741 agcagccgcc cgatgtcgtc tcggcagctc gcgacgccgc gctgatctcc ggagctctcg
     1801 aacaacgcga gcgcggtttc gaagtgcctt tgggccgggc cgagcgagcc ggtgtcgcgg
     1861 tacaggcgcc cgatgcggtt gtgcgcggcg ccccccttcg agcgcagtcc gaggcggtaa
     1921 gcgatgccga gcatctcgcg gaacgcggcg agcgcctcgt cggtgcgccc gagcagcacg
     1981 agcacgtcgc cgtgattgtg gagcgcgtcg atgcggcgtc gcgcgtcgtc ttcgccgagc
     2041 agctcgaggc cctttcgata gtattcgtcg gcgcgcttcg ctgcgaagtg ctcgcgcgcc
     2101 gtgttgccgg actcgatgta cgtgaacgcg gcgcgcgtga gggagcccgc gtgctcgagg
     2161 tgtcgcgcga gcatcgcgct gtactcctct tgattgcgca cgttgtcctt ctgcgcgagc
     2221 caatccgcga tggtttggtg gtagcggcgc gccgacgggg ccgcggtcaa ccgcgcgatc
     2281 gtctcgcgct cgaggttgtg tttgaagacg tactcgacct caccagggaa ggcactgtcc
     2341 ggcaccttga gcacgtagtc gcgcgccgtg agctcgctca gcgcgttttc gaccgcgctg
     2401 acgtcatcgg tgccgctctc ggcccagagc tccggcggtt ctctgtcgat gcgaccgagg
     2461 gccacgagcg cgctcagcca gaatacgctg cccatggtcg ctgcgtgctc gagcagccga
     2521 cgttcgtgcg gtgcgagcgc ggcgatgcga acctgcaccg cgtcttcgac ggtgagaggg
     2581 agccgtacgc tcgcgaggcg gtccaagtcg acgcgccagc cgccgccaac gtcgttcgga
     2641 tcctcgagca gcacgccggc gtcgcgaaat acgctgacca tgtgctcgag cagccccggc
     2701 gcgccgcccg ccatgctgac ggcggcttcg accaaccgcg gcggcggccc cccctgacag
     2761 cgagcgagca acgactgcat gatcgcgatc gcgtcgtctt cggcgacggg ccccacctcg
     2821 agcagagtgt gccgcgaacc agcgagggcc gcccactcgg tgtggcgact cagaaactcg
     2881 gggcgcgtgt tgcagacgac gagcaacttg ccgcgca
//
LOCUS       DASTKS010000188         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_920744, whole genome shotgun sequence.
ACCESSION   DASTKS010000188 DASTKS010000000
VERSION     DASTKS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920744"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFQ35_03960"
     CDS             <1..449
                     /locus_tag="VFQ35_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9929812.1"
                     /translation="KAKLTQMGIEPTHAEIAKRLNVEESEVIEMDRRLSRADASLDAP
                     VGEGDDKSTTRLDLLPATAESPDTLTEDAEMQQILREKLGAFRTTLTGKDVAIFDKRL
                     VADEPLTLQELGDEFGVSRERVRQLEARLTGKLRAYLQQELGDGVA"
     gene            453..1319
                     /gene="rsmI"
                     /locus_tag="VFQ35_03965"
     CDS             453..1319
                     /gene="rsmI"
                     /locus_tag="VFQ35_03965"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9929813.1"
                     /translation="MAGTLYLVATPIGNLQDITLRALETLRAASLIAAEDTRRTRALL
                     THFAISGKHVVACNAHASPEELAGLVERLRAGENVALVTDAGTPSVSDPGTPLVRSAV
                     AAGIGVVPIPGVSAVTTAVAASGLVDGPFRFVGFLPSKTSRRSALLSEVALSREPCVL
                     FEAPHRIAETLRDLATLAPEREAVVCRELTKLHEELLRGSLATLAAKAESAPLRGEIT
                     LVVAAAPESELKAPLDEAAIDESILSGLRAGESPRSVVEALAAACPMPRRELYRRVTE
                     LAQKLSAEAVDE"
     gene            1410..1955
                     /locus_tag="VFQ35_03970"
     CDS             1410..1955
                     /locus_tag="VFQ35_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9929814.1"
                     /translation="MVVLIFAHPYPDRSRANRTLLAAVRDLPEVEVRSLYDLYPGFDF
                     DVPAEQAALLRSKIVVWQHPIYWYAPPGLLKHWFDKVLLRGWAYGEGGDALHGKRCLW
                     VATTGGETSAYGPEGMHGHPFSAFVPAVEQTARFCGMRWESPIVLYGAHRVSDAALGR
                     AAEEYRARLVELIEQVKRSEP"
     gene            1952..3790
                     /gene="kefC"
                     /locus_tag="VFQ35_03975"
     CDS             1952..3790
                     /gene="kefC"
                     /locus_tag="VFQ35_03975"
                     /inference="COORDINATES: protein motif:HMM:NF002924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HET9929815.1"
                     /translation="MNEHSTLTDVIVYLIAAVVCVPLASRSKLGSVLGYLAAGCIIGP
                     FGLGLIQDAQETMHFAEIGVVLMLFVIGLELDPKQLWTMRRSVFGGGGLQLVAAAIPL
                     GTGAFALGLPWQAAVVTGLALGLSSTAVSMQTMQEKGLLGSGVGKTAFSILLFQDIAA
                     IPLLALVPLLAEPQAKQGGGGWLAAVKILSAVLAVFVIGRFLTRPVLRAIARTGLREV
                     FTAFTLLLVLGIAQIMATVGISMGLGAFLAGVLLAGSEYRHALETDIQPFKGLLMGLF
                     FIAVGMSINFGLFAQKPLLVAVLVVGFQILKGVSLRVVAGPLGITKPQRWLFAALLTQ
                     GGEFAFVVFGVARTARLLPDDWDPLLTLAVALSMAFTPLLLIVHDRLMLRAQTGEREP
                     DSIEDAHAPVIVAGFGRFGQIVGRLLFASGIKATVLDHDADQIELLRRFDFKVYYGDA
                     TRLDLLHAAGAEHARLLVVAIDDVESSLRLVEAAREHFPNLPIIARARNVSHWSALRR
                     HGVEIVERETFEGALSLGQKALQALGVAPYEARERADSFRRHNVRMLEEILPHWADQE
                     RRTSIARSAREQLERQMDRDRAELDRHGAPGWQGDSDLVDQEHTGS"
     gene            3978..>4268
                     /locus_tag="VFQ35_03980"
     CDS             3978..>4268
                     /locus_tag="VFQ35_03980"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9929816.1"
                     /translation="MRGQEKKQTSMLVLRSPEDFVPKQHPIRRIQKLADDVLRALSPT
                     LEAMYAQGGRPSIPPERLLKATILMALYTVRSERQFSEQLNYNLLFRWFLDMD"
BASE COUNT          675 a         1380 c         1427 g          786 t
ORIGIN      
        1 agaaggcgaa gctcacgcaa atgggcatcg agcccaccca cgccgagatc gcgaaacgcc
       61 tgaacgtcga ggagtcggag gtcatcgaaa tggaccggcg cctctcacgc gccgacgcat
      121 cgctcgacgc tcccgtgggc gagggcgacg acaagtccac gacgcgcctc gaccttctgc
      181 ccgcgacggc cgagagcccc gatacgctca ccgaagacgc cgaaatgcag caaatcttgc
      241 gggagaagct cggcgccttc cgcacgacgc tcaccggtaa ggacgtcgcc attttcgata
      301 agcgcttggt ggcagacgag ccgctcacgc tccaagagct gggggacgag ttcggagtgt
      361 cgcgcgagcg cgtgcgccag ctcgaggccc gcttgacggg caagctccgc gcgtacttgc
      421 agcaagagct cggcgacggc gtggcgtgag tcgtggccgg tacgctctat ctggttgcga
      481 cgcccatcgg caacctgcaa gacatcacgt tgcgcgcgct cgagacgctg cgggccgcgt
      541 cgctcatcgc agcagaagac actcggcgca cccgggcgct gctcacgcac ttcgcaatca
      601 gcggtaagca cgtagtcgcg tgcaacgctc acgcgagccc cgaagagctg gcaggtttgg
      661 tcgaacggct gcgcgctggc gagaacgtag cgttggtgac ggacgcgggt acgccgtccg
      721 tgagcgaccc tggcacgccc ctcgtgcgct ctgcggtcgc ggcaggcatc ggcgtggtcc
      781 ccattcccgg cgttagcgcc gtcaccaccg ccgtcgccgc gtcggggctc gtggacgggc
      841 catttcggtt cgtcggcttt ttgccgtcga agacgagccg ccgcagcgct ctactcagcg
      901 aagtcgcgtt gagccgtgag ccgtgcgtgc tgttcgaggc tcctcaccgt atcgcggaga
      961 cgctgcgcga tttggcgacg cttgcgcccg agcgcgaagc cgtcgtttgc cgcgagctca
     1021 cgaagctcca tgaggagctc ttgcgtggca gtttggccac gttggcggcg aaggccgaga
     1081 gcgccccgct gcgcggcgaa atcaccctgg tcgtggccgc tgctcccgag tcggaattga
     1141 aggctcctct cgacgaagca gcgatcgacg agagcatctt gtcggggctc cgcgccgggg
     1201 agtctccgcg tagcgtggta gaagcgctgg cggctgcttg cccaatgcct cgacgtgagc
     1261 tctaccggcg cgtgacagag ctcgcgcaga agctctccgc cgaagccgtc gacgagtagc
     1321 gtcgagggct cgtacgactt cgctcccgcg cgctccccgg atggctggtt gtcgcccgaa
     1381 cgcggctgtg cgcacttaag cgcagagcca tggtcgtctt gatcttcgcg cacccgtatc
     1441 cggaccgctc tcgggccaac cgaaccttgc tcgccgccgt gcgcgacttg cccgaggtcg
     1501 aggtgcgttc gctctacgat ctttatcccg gcttcgactt cgacgtgccc gcggagcaag
     1561 cggcgttgct tcgctcgaag atcgtcgtct ggcagcaccc gatatattgg tacgctccgc
     1621 ctgggctctt gaagcattgg ttcgacaagg tgctcttgcg cggttgggcc tacggagagg
     1681 gcggtgatgc gctgcacggc aagcgttgct tgtgggtcgc gaccactggg ggcgaaacgt
     1741 ccgcgtacgg tcccgagggc atgcacggcc atccgttctc ggctttcgtg cccgcggtcg
     1801 agcagacggc gcgattctgc ggaatgcgtt gggagtcgcc catcgtcttg tacggagcgc
     1861 atcgggtatc agacgccgcg ctcgggcggg ccgcggaaga gtatcgcgcg cgcctcgtcg
     1921 agctcatcga gcaagtgaag cggagcgagc catgaacgaa cactcgacgc tcacggacgt
     1981 catcgtctat ctcatcgcgg ccgtcgtttg cgtgccgctc gcgagtcgca gcaagctcgg
     2041 ctcggtgctc ggctacctag ccgcgggctg catcatcggc ccgttcgggc tcggcttgat
     2101 tcaagacgca caagagacga tgcacttcgc cgagatcggc gtcgtcctga tgctgttcgt
     2161 gattgggctc gagctcgacc ccaagcagct ctggacgatg cgccgttcgg ttttcggtgg
     2221 gggagggctg cagctcgtcg cggccgcgat tccgctcggg acgggtgcgt tcgcgctcgg
     2281 gttgccctgg caggcggcgg tcgtgactgg gcttgcgctc gggctctcgt cgaccgcggt
     2341 gtccatgcaa accatgcagg agaaggggct cctcgggagt ggcgtcggca agacagcgtt
     2401 ttcgatcctg cttttccagg acatcgccgc gatcccgttg ctcgcgctcg tgccgttgct
     2461 cgctgagccg caggccaagc aaggtggcgg gggttggctc gcggcggtga agatcctatc
     2521 cgccgtgtta gcggtgttcg tcatcgggcg ctttctcacg cggccggtgt tgcgagccat
     2581 cgcccgcacc ggcctacgcg aggtgttcac cgcgttcacg ctgctgctcg tgctcggcat
     2641 cgcgcaaatc atggcgacgg tcgggatctc gatggggctc ggcgcgttct tggcaggcgt
     2701 cttgctcgcc ggctcggagt accggcacgc gctcgagacc gatattcagc cgttcaaggg
     2761 ccttttgatg ggactcttct tcatcgcggt tgggatgagc atcaacttcg ggctgttcgc
     2821 gcaaaagcct ctgctcgtcg ccgtcttggt cgtcggcttc caaatcttga agggcgtttc
     2881 gctgcgcgtc gtcgcgggcc cgctcggaat caccaagccg cagcgctggc tcttcgcggc
     2941 gctgctcact caagggggag agtttgcgtt cgtagtgttc ggagtcgccc gcaccgcgcg
     3001 tctcctgcca gacgactggg acccactgct cacgctcgcg gtcgcgctgt cgatggcgtt
     3061 cacgccgctg ctgctcatcg tgcacgaccg gctcatgctg cgcgcccaaa ccggtgaacg
     3121 cgaacccgac agcatcgaag acgctcacgc gcccgtcatc gtcgcgggct tcgggcgctt
     3181 cggtcaaatc gtcggtcgat tgctcttcgc gagcggcatc aaggcgacgg tgctcgacca
     3241 cgacgccgat cagatcgagc ttttgcggcg cttcgacttc aaggtctact acggcgacgc
     3301 gactcggctc gatctgttgc acgcggccgg cgcggaacat gctcgcctct tggtggtcgc
     3361 catcgacgac gtggagtcca gcttgcggct cgtcgaggcc gcgcgcgagc actttcccaa
     3421 cctgccgatc atcgcgcgcg cacgcaacgt gtcgcactgg tcggcgctcc ggcgccatgg
     3481 cgtcgagatc gtcgagcgcg agaccttcga gggtgcgctc agtctcggcc aaaaggctct
     3541 ccaagcgctg ggcgttgcgc cttacgaagc acgcgaacga gcagactcgt tccgccgcca
     3601 caacgtccga atgttggaag aaatcctgcc gcattgggcg gatcaagagc gacgcaccag
     3661 catcgcccgg tccgctcgcg agcagctcga gcgtcaaatg gaccgcgacc gcgcggagct
     3721 agatcgccac ggggcgcccg gctggcaggg agacagtgat ctggtcgatc aagagcacac
     3781 cggctcttag cagggtgttg aaaaacggca gcggctcggg atcgtataac ggcagcattc
     3841 ggttgtgatg cccacccccg tccgtgctcg gcctacggcc tgcgcgcggc cgacccctcc
     3901 caaatcgcgc gcaagcgcgc tcagggaggg gtgagcgctg gatcgacatc cggatctgga
     3961 atggataggg tctggtcatg cgcggccagg aaaagaagca aacgtcgatg ctggtgcttc
     4021 ggtcaccgga ggacttcgtg ccgaagcagc atccgattcg gcggatccag aagctggccg
     4081 acgatgttct tcgtgcgctg tcgccgacgt tggaggccat gtacgcgcag ggcggccgac
     4141 cgtcgatccc gccggagcgc ttgctcaagg cgacgatctt gatggcgctc tatacggtgc
     4201 gcagcgagcg gcagttctcc gagcagctca actacaacct gctgtttcgc tggtttctcg
     4261 acatggac
//
LOCUS       DASTKS010000189         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_250871, whole genome shotgun sequence.
ACCESSION   DASTKS010000189 DASTKS010000000
VERSION     DASTKS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250871"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFQ35_03985"
     CDS             <1..238
                     /locus_tag="VFQ35_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929817.1"
                     /translation="EGTDRGLVPLRELRTRVAQRARLEHPSWSPTWVTPVDRARRAAT
                     SHPTPLATRTATLVSARLAGEPTETPKPRRYERP"
     gene            375..1490
                     /locus_tag="VFQ35_03990"
     CDS             375..1490
                     /locus_tag="VFQ35_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9929818.1"
                     /translation="MAARPTVCLNMIVKNEAKVIARSLASTRPFIDAWAIVDTGSSDG
                     TQDVIRQELRDVPGELFERPWRDFGTNRSEALELAKGRADYTLIIDADEVLAPSPGFG
                     LPELTLDAYQLRTEFGGVRYYRTQLVRSSLPWRYEGVLHEVLACDASFTQGKLEGLVN
                     RPQSDGARSADPQKYVKDAAVFEQALKREPHNTRYVFYLAQSYRDAGLDEAALVNYAR
                     RAKLGGWDEEVWYSMFQVASLLERLARPEHEVVIAYLDAHQFRPQRAESLCELARYLR
                     TKKKFALAYVFARQAADTPMPSDTLFVHEGTYSWRARDELSIAAYYVGKHEEAITLTT
                     ALLDGATLPEAERARVMKNREFSLNALKPNQKGTAGG"
     gene            complement(1875..>2364)
                     /locus_tag="VFQ35_03995"
     CDS             complement(1875..>2364)
                     /locus_tag="VFQ35_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929819.1"
                     /translation="FYAYSMQIAPPNFMNVGGTLIVATYSSGVYSVNVTTGAYTQIAP
                     NTIGSALNAMDFALDGTLYISAWGTNALVSISPTGVASSVVAIQRPSSVQVDDARNRV
                     LVYSEQTSGGAIQAVDRTTFAVTTLASSLGSNAGSAQTSLISDGTNHAVLRTSSSITL
                     LP"
BASE COUNT          467 a          764 c          763 g          370 t
ORIGIN      
        1 cgaggggacc gaccgcgggc tcgtccccct tcgcgagttg cgcactcgag ttgcacaacg
       61 tgcgcgactc gagcatccaa gttggtcacc cacatgggtg actcccgtgg accgagcgcg
      121 ccgcgccgcg acctcgcacc ccactccact cgcgactcgc acggcgacgc tcgtctcggc
      181 gcgcctcgcg ggtgagccga ccgagacccc gaagccgcgc cgttacgaaa gaccgtgagc
      241 gcagcgcgct cgttcgagtc agtgcgtgct tttgctgggg cctcgacgcg ctccgactcg
      301 gcatttggcc cagcgttcga ggagccgaga gacatcgcgc ctacactggc gcaggtttcc
      361 gatatgctcg ccgaatggcg gcgcgtccga cggtgtgcct gaacatgatc gtaaagaacg
      421 aagccaaagt gatcgcgcga tcgctcgcct cgacccggcc gttcatagac gcctgggcga
      481 tcgtcgatac ggggtcgagt gacggcactc aagacgtgat ccggcaggaa ctgcgggacg
      541 tccccggcga gctcttcgag cggccctggc gagacttcgg tacgaatcgc agcgaagcgc
      601 tcgaactcgc gaaggggcgc gccgactaca ctttgatcat cgacgctgat gaggtgctcg
      661 cgccgagccc tggtttcggc ttgccggagt tgacgctcga cgcatatcag ctgcgcaccg
      721 agttcggggg ggtgcgctat taccgcaccc aattggttcg ttcgagtttg ccttggcgtt
      781 acgagggggt actgcacgag gtcctcgcgt gcgacgcctc gttcacccaa ggcaagctcg
      841 agggcttggt gaaccggccg caaagcgacg gcgcgcgctc cgccgacccg caaaagtacg
      901 tcaaggatgc ggccgtattc gagcaagcgt tgaagcgcga gcctcacaat acgcgctatg
      961 tcttctatct cgcccagagc tatcgtgacg ccggcctcga cgaagccgcg ctcgtcaact
     1021 acgcgcgacg ggccaagctc ggcgggtggg acgaggaagt gtggtactcg atgtttcagg
     1081 tcgcctctct gctggagagg ctcgcgaggc ccgagcacga ggtcgtgatt gcgtacctgg
     1141 acgcccatca gttccgcccc caacgcgccg agagcctatg cgagctcgcc cgctacttgc
     1201 ggacaaagaa gaagttcgcc ctcgcctacg tgtttgcacg gcaagccgcc gacacaccaa
     1261 tgcccagtga caccctgttc gtgcacgaag gcacctattc gtggcgggcg cgcgacgaat
     1321 tgtccatcgc agcgtactac gtgggcaagc acgaagaagc gatcaccctc acgaccgcgc
     1381 tgctcgatgg cgcgacacta cccgaagccg agcgcgcccg cgtgatgaag aatcgcgagt
     1441 tcagtctcaa cgcgctgaag ccaaaccaga agggaaccgc tggaggctaa gggcccgcga
     1501 atggataagt cagtcgcgag cgagccgaga ccgcagcgag gcgggcgcgt gaggagagag
     1561 caccgcaggc ctgctgaagc aggtcgagga gcgcgacgac gagcccgccc gccgcagcaa
     1621 gaggatcggc ccgcgcagta tgacctgccc attcgcgggc cctaacgcca cgaccagcga
     1681 agaaccgaac cccgcgcgac cggccggacg agccccttca agcgccagcc gagcgaagaa
     1741 cgaacgcaag ccctaacccg tcggagacga cgcctcgggt tggaacggcg actctctcca
     1801 gagcgaccgt cccagcccgg acgacgatgc catcacggtg ccgtgagccg caccgcgaca
     1861 gttccgtaaa gacactacgg aagcagggtt atcgacgagc tcgtgcgcag caccgcgtgg
     1921 ttcgtaccgt ccgagatcaa cgacgtttgc gctgaacccg cgttcgagcc gagagagctc
     1981 gccagggtcg tgacggcgaa agtggtgcga tcgactgcct gaatcgcgcc accgctcgtc
     2041 tgctcggagt aaacgagcac gcggttcctg gcgtcgtcga cctggaccga cgatggacgc
     2101 tgaatggcga cgacgctact cgcgacgccg gtgggagaga tggagaccaa cgcgttcgtt
     2161 ccccatgcgc tgatgtagag cgtgccatcg agtgcgaagt ccatggcatt gagcgcgcta
     2221 ccgatcgtgt ttggcgcgat ctgcgtgtac gcccccgtcg tcacgttgac ggaatagaca
     2281 cccgaggagt acgtagcgac gatcaacgta ccaccgacgt tcatgaagtt cggtggcgcg
     2341 atctgcatgg aataggcgta gaag
//
LOCUS       DASTKS010000190         6104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1367674, whole genome shotgun sequence.
ACCESSION   DASTKS010000190 DASTKS010000000
VERSION     DASTKS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367674"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(578..1159)
                     /locus_tag="VFQ35_04000"
     CDS             complement(578..1159)
                     /locus_tag="VFQ35_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9929820.1"
                     /translation="MAISFPLALHLDDKPCLVVGSDVEARDRAAALRDAGALVEIIAQ
                     TPCPELVELAATRSVRLEQREFSPDDLNGKWLAVLVDRNNELGARLAALCDDRRILFC
                     AVDQQQKNSFSHMALVRRGVITIAISTMGRVPALGRRLREELERVFDESGLAAFADRL
                     AALRDATPPEKRRTVLGEVTRAVRFEGRLKLEP"
     gene            complement(1260..1856)
                     /locus_tag="VFQ35_04005"
     CDS             complement(1260..1856)
                     /locus_tag="VFQ35_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9929821.1"
                     /translation="MTKVSASDYRALAAFRYEIRKFLAFSEVAARAAQIEPQQHQLLL
                     AVQGLPPDQRPTIRAISERLCVRHHTTVALVDKLEFHGLLQRERSQQDRREVLLRLTP
                     QGSAKLLALSILHKDQLRAVGAHMLTALGEILGELEPSTPRSKPQAPRAKSKLAKADT
                     NGGSAAPRSPRGRESKSSALLTKGSNGKRPSGRSKAPA"
     gene            complement(2119..2766)
                     /locus_tag="VFQ35_04010"
     CDS             complement(2119..2766)
                     /locus_tag="VFQ35_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HET9929822.1"
                     /translation="MIALGALLRTPVVVAQQQPPPRAVSLEWDTDEKLLYLSVGFRDV
                     IDDKIQEKLSRGLPTTIVLTAALYRANAQEAISTTAQTCKVTWHVWEEAYRVEITRPG
                     GSQVRWTTTIEGVLRRCGEAHRLLAGDRSQIPEGGPLRVVGKIQVNPISPEVLTKIQR
                     WVTRPASTGTAAPGDALFSTFTGLFLQRIGDAERDVKFATRPLLPSVFRSRREDR"
     gene            complement(2851..4578)
                     /locus_tag="VFQ35_04015"
     CDS             complement(2851..4578)
                     /locus_tag="VFQ35_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9929823.1"
                     /translation="MLRSIVVFRGRSVSSIALLAASLALSALVRAQTAPPEPEAPASP
                     TAPDAEPRSQPDAAAPDTWGTQPAPASEPERPPAATPAPPPSAPSAAEVLASRPCNDC
                     SPRARDESSVRYTLENIEVRGNTRTSTRVILRYVRFKAGDVLDVDDPEVQLTRFRLLG
                     TGFFRDVQFSLRKGSERGRVVLVVDVVERNTIVVNDIAMGLAADADAKGHARHLAAYA
                     GLDVAETNLGGSGITLGGAAAVGGAATNVAPQIALRVRFLDPAFLGGPWMTSGVLLFN
                     DAQDSFGYGVQKWNHPGGPEYADGPAIVRYKRFGGSIGVGRDLSISTQFWATYRLETI
                     DANLPLAASQFRGFLEEPVDFHLVRGRSVLSTLSGTLQLDTRDHPFLPTRGWFVSFRG
                     ELSLLPAALDYDYQKFELDASHWWKLPFHDHVLKLGVFAGGISGHAPFFEQFYVGDYS
                     DLLPDRVLGLNFDRRPAPNFLGTDIVEVRYGDFALKLNAEYRIPIYRGRRSIYGVDFF
                     ATTGIYGVAGRRDLTEPPTGYSGLARFPLDVTANVGLRMDTSAGGFAFAFSNVLSFVP
                     VRGQGPAGQ"
     gene            complement(4681..>6104)
                     /gene="malQ"
                     /locus_tag="VFQ35_04020"
     CDS             complement(4681..>6104)
                     /gene="malQ"
                     /locus_tag="VFQ35_04020"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET9929824.1"
                     /translation="QSWWQMLPVGPLGGGESPYDSPSTFAGNPLLVSLEFLARDGLLD
                     RHDLVAHPRLVNAKRCLWPSVRRYRQARLRRAFERFVPRSRGDLAHEFENFRHLSRDW
                     LRGYALFRALKRAHGERPWTEWERPLALRETEAIERAERQLADEVAFQEFVQFAFHRQ
                     WEGLRARCRELGIRLLGDVPMFVAHDGADVWQHREIFQLDDRGHRRVVAGVPPDYFCA
                     EGQLWGNPIYDWPALEQRGYDWWLARMRTTLSRFDAVRLDHFIAFHRYWEIPASAPTA
                     REGRFVSVPGVDFFTKLEQAVGGLPFIAEDLGLVTPEVHALRERFNLPGMRVLEFGFA
                     GDFRDYQPHRYPKDTVVYTGTHDNDTLVGWLSAHARATDPDERRALVAERERALRYAG
                     SGNEEEHWRFIRLALSSVANTALFPLQDVLGQGSEARMNVPGTASGNWCYRFQAEALT
                     PEIAERLHSLSDTYERIPRRHPR"
BASE COUNT         1033 a         1898 c         2113 g         1060 t
ORIGIN      
        1 tcgctgctcc gggttcgctg ctccgggttc gctgctccgg gttcgccgct ccgggttcgc
       61 cgctcgcgat gcttctgtgt tgctcgtcgg ttgggggagc taccgcagcg cattgtgacg
      121 ttttgacata atttgtcaca atgttccaag cgggtggaaa ggcacagctc cagccatctc
      181 gccggtgtcg aggtaagtcg aggcgtgctc ggtccctgtc tcagaacctc gaggcggcgc
      241 gtttggaatt cgtgcgtttg gatccgagga attcaaggcg tgcgcgtttt ggaattcgtg
      301 cgcgtttgga aggcgtgcgt ttggaaccgt ggaatccaat gcgtgcgcgt ttgccggcta
      361 gttggggggt tcttttccga ttgcgacgtt ttgacaaaaa atgtcacaac gtcgcgacgg
      421 cgtagggagt atcactgctc cagcagtttc gttgtcgccc agccactcgc ggcgcgcggc
      481 gggcacgcgc cgcccagcca ctcgcggcga gcggcgcgcg gcgggcacgc gggtcgtgcg
      541 atggggctga cgggggaggc ggcgtgcgtg tcgttcgcta tggctcgagc ttgaggcgac
      601 cttcgaagcg cacggcgcgg gtcacttcgc cgagcacggt gcgacgcttt tccggtggcg
      661 ttgcgtcgcg cagcgccgcg agtcgatcgg cgaacgctgc gagcccggac tcgtcgaata
      721 cgcgctcgag ctcttcacgc aaacgccgtc cgagcgccgg gacgcgcccc atcgtgctga
      781 tggcgatcgt gatgacccct cgtcgcacta gcgccatgtg cgaaaaggag ttcttttgct
      841 gctggtccac cgcgcaaaac aaaatccgac ggtcgtcgca gagtgccgcc agccgcgctc
      901 cgagctcgtt gtttcgatcg accaacaccg cgagccattt cccattcaag tcgtccggtg
      961 aaaactcgcg ctgctcgagg cggacgctac gagtcgcggc gagctcgacg agctccggac
     1021 acggcgtctg cgcaattatt tcgacgagcg ctcctgcgtc ccgaagcgcc gccgctcgat
     1081 cacgcgcctc tacgtcggag cccactacga gacatggctt gtcgtcgagg tgtagagcga
     1141 gggggaacga aattgccacc cgccaagcct agccggggat gaaaaggatt catcccatgt
     1201 cgagccgccc aggcgctgcc ggtccgggcg gggcgcgccg tttcgtcgtt tctcgctgac
     1261 taggccggtg ctttgctgcg accgctcggt cgtttgccgt tcgaaccctt cgtgagaaga
     1321 gcgctcgact tgctctcgcg gcccctcggt gagcgcgggg cagcgctacc gccattggta
     1381 tcggcttttg cgagcttgct cttggcgcgc ggcgcttgcg gcttgctgcg gggcgtcgaa
     1441 ggttctagct cgcccagaat ttcgccgagc gcggtcagca tgtgggcgcc gactgctcgc
     1501 agctggtcct tgtgaaggat ggagagtgcc agcagcttcg ctgagccttg gggggtgagg
     1561 cgcagcaata cttcgcgtcg atcctgctgg ctccgctcgc gctgcaagag cccatgaaac
     1621 tcgagcttgt ccacgagggc taccgtggtg tggtgacgca cgcaaagacg ctcagaaatc
     1681 gcgcggatcg tcgggcgctg gtccggcggc agcccctgca cagccaaaag cagctggtgt
     1741 tgttgcggct cgatttgcgc ggctcgcgct gcaacctcac tgaatgccag gaattttcgg
     1801 atttcgtagc gaaacgcggc gagcgcgcgg tagtccgagg cggaaacctt cgtcacccaa
     1861 attctcgtag cgggctcgcc ccgtctggtc aacccgttcg gccggaggag atgacgcaat
     1921 gagtgcaagg aaccgcagaa acgacaagtg tcgtcattcg cgacgggcgc acttcgcatt
     1981 tcgctggatg aattgccgag gcgacttcgg gggcgccctc ggttttccct ccgtggatag
     2041 cggttggaca caccgttccg tgggcccatt ccttcatggc gccaaaacat gctttaggcc
     2101 ggagtgcgtc tcgggcggtc agcggtcttc gcggcgcgag cggaacacgc tgggtagcaa
     2161 agggcgtgtt gcaaacttga cgtcacgctc ggcgtcgccg atgcgctgca ggaacaagcc
     2221 ggtgaacgtg ctgaagagcg cgtctccagg ggcggcggtg cccgtgctcg cggggcgcgt
     2281 aacccagcgc tggatcttgg tgagcacctc ggggctgatc gggttcacct ggatcttgcc
     2341 gaccacgcgt agggggccgc cctcggggat ttgggagcga tcgccggcga gcaaccggtg
     2401 cgcttcaccg cagcggcgaa gcacgccttc gattgtcgtg gtccagcgta cttggcttcc
     2461 gccgggtcgc gtgatttcaa cacggtacgc ctcttcccaa acgtgccaag tgaccttgca
     2521 ggtctgagcg gtcgtcgaga tcgcctcctg cgcgttcgcg cggtagaggg cggcagtgag
     2581 cacgatggtc gtcggcagtc cgcggctcag cttctcctga atcttgtcgt cgatgacgtc
     2641 acggaaccca acgctcagat aaagtagctt ttcgtcggta tcccactcga ggctcactgc
     2701 gcgcggaggc ggctgctgtt gcgcgactac gactggagta cggagcagcg ctccgagcgc
     2761 gatcacgact ccgagaagcc aggcttgaaa acctggcgac ggtcgaagcc tgtgagcagg
     2821 tcgtcgcgcc acggtgctca tccggcgggc ctattgcccc gctggcccct gaccccgaac
     2881 gggcacgaaa ctgagcacgt tggagaacgc gaacgcgaag ccacccgcgc tggtatccat
     2941 ccgcaacccg acatttgccg tcacgtcgag ggggaagcgc gcgaggccgg aatagcccgt
     3001 gggcggctcg gtgagatcgc ggcgaccagc gactccgtag atgccggtcg tggcgaagaa
     3061 gtcgacgccg taaatcgagc gacgccctcg atagatcggg attcgatact ctgcgttgag
     3121 cttcaacgcg aaatcgccgt aacgaacttc gacgatgtcg gtgccgagga agttcggcgc
     3181 cggacgtcga tcgaagttca acccgagcac gcgatcgggg agcagatcgc tgtagtcgcc
     3241 gacgtagaac tgctcgaaga aaggggcgtg cccagagatg ccgcccgcga acacgccgag
     3301 cttcaacacg tgatcgtgga agggcagttt ccaccagtgc gaggcgtcga gctcgaactt
     3361 ttgataatcg taatcgaggg cggccggcaa gagcgagagc tcaccgcgga acgacacgaa
     3421 ccaaccgcgc gtgggcaaga agggatgatc gcgagtgtcg agttgcagcg tgccgctgag
     3481 tgtcgagagc acgctccgtc cgcgcacgag atggaagtcg accggctcct cgaggaagcc
     3541 gcgaaattgc gaggcggcga ggggcaggtt cgcgtcgatg gtttcgaggc ggtaagtcgc
     3601 ccaaaactgc gtcgatatgc tgagatcgcg cccgacgccg atcgagccgc cgaagcgctt
     3661 gtagcgcacg atggcggggc cgtcggcgta ttcgggcccg cccgggtggt tccacttctg
     3721 cactccgtag ccgaacgaat cctgggcgtc gttgaagagc agcacgccgc tcgtcatcca
     3781 cggtccgccg aggaaggctg gatcgaggaa gcggacgcgc agcgcgattt gcggcgcgac
     3841 gttggtcgcg gcaccgccca cggcggccgc accgccgagc gtgatgccgc tgcctccgag
     3901 gttcgtctcg gcgacgtcga gccctgcgta cgcggccaag tgccgcgcgt gccctttcgc
     3961 gtcggcgtcc gcggcgagcc ccatcgcgat gtcgttgacc acgatggtgt tgcgctccac
     4021 cacgtcgacc acgagcacca cgcggccccg ctcgcttccc ttgcggagcg agaactgaac
     4081 gtcgcggaag aaccccgttc cgagcaggcg aaagcgcgtg agctgcactt ccggatcgtc
     4141 gacgtcgagc acgtcgccgg ccttgaagcg cacgtagcga agaatcactc gagtcgaggt
     4201 gcgcgtattg cctctgacct cgatgttttc gagggtgtag cgcacgctgc tctcgtcacg
     4261 tgctctcggc gagcagtcgt tgcagggccg agacgcgagc acctccgccg cgctgggtgc
     4321 gctcggcggg ggggcggggg tagcagcagg ggggcgctcc ggctcgctcg caggagccgg
     4381 ctgcgtgccc caagtgtccg gagccgcagc gtcaggctgg ctgcggggtt ctgcgtccgg
     4441 agcggtcggg ctggcaggag cctccggctc cggcggggcc gtctgggcgc gcaccaacgc
     4501 cgagagcgcg agcgatgcgg cgagcaaagc aatggacgaa accgagcgcc cgcggaacac
     4561 gacgatggag cgtagcactg gcgccgggtt caacgcgcca ggaatttcga ggcgcgcggt
     4621 gaggagccgt cgcgcaggga gcggtgaaga gccgtcgcgg cgggagcggt gaagagccgc
     4681 tcagcgcggg tgccggcgtg ggatgcgctc gtaggtgtcg gagagcgaat gcagccgctc
     4741 tgcgatttcg ggcgtgagag cctcggcctg gaagcgatag caccagttgc cgctcgcggt
     4801 gccgggcacg ttcatgcgtg cctcactgcc ctgaccgagc acgtcttgca gggggaaaag
     4861 tgcggtattg gcgaccgagc tcaacgcgag ccggatgaag cgccagtgct cctcctcgtt
     4921 cccggagcct gcgtagcgga gtgcgcgctc acgctcggcg acgagggcgc ggcgctcatc
     4981 gggatcggtt gcgcgcgcgt gggcggagag ccaaccgacc aacgtgtcgt tgtcgtgcgt
     5041 ccccgtgtag accaccgtgt ccttcggata gcgatggggc tggtagtcgc gaaaatcccc
     5101 ggcaaacccg aactccagca cgcgcatgcc gggcaggttg aagcgctcgc gaagcgcgtg
     5161 gacctcgggc gtcacgagcc ccagatcttc agcgatgaaa ggcagccctc ccaccgcttg
     5221 ctcgagcttc gtgaaaaagt ccacgccagg tacgctgacg aagcgtcctt cgcgcgcagt
     5281 cggcgccgaa gccgggatct cccagtagcg gtggaacgcg atgaagtggt cgagccgcac
     5341 ggcgtcgaaa cgcgaaaggg tggtgcgcat gcgcgcgagc caccagtcgt agccgcgctg
     5401 ttcgagggcg ggccaatcgt agatggggtt tccccacagc tggccttcgg cgcaaaagta
     5461 gtccggcggt accccggcca cgacgcggcg atgtccccgg tcgtcgagtt gaaagatctc
     5521 tcggtgctgc cacacgtcgg cgccgtcgtg cgctacgaac atgggcacgt cgccgagcaa
     5581 acgaatgccg agctcgcggc agcgcgcgcg cagcccctcc cattgtcggt gaaaggcgaa
     5641 ttgcacgaac tcctgaaagg cgacctcgtc ggcgagctgg cgctcggccc gttcgattgc
     5701 ttcggtctcg cggagcgcga gcggtcgctc ccattcggtc cacggtcgct ccccgtgcgc
     5761 ccgcttcagt gcgcggaaca gcgcgtaccc ccgcaaccag tcgcgtgaca ggtggcgaaa
     5821 attttcgaac tcgtgtgcaa gatcgcctcg tgatcgcggc acgaagcgct cgaacgcgcg
     5881 acgcaggcgc gcttggcggt agcgccgcac cgacggccag aggcagcgct tcgcgttgac
     5941 gagccgtgga tgggcgacca gatcgtggcg atcgagcaag ccgtctctcg ccaggaactc
     6001 gaggctgacg agcagagggt tgccggcaaa cgtggacgga ctgtcgtacg gcgattctcc
     6061 ccctccgagc gggccgactg ggagcatctg ccaccaggac tggc
//
LOCUS       DASTKS010000191         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_698522, whole genome shotgun sequence.
ACCESSION   DASTKS010000191 DASTKS010000000
VERSION     DASTKS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698522"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFQ35_04025"
     CDS             <1..731
                     /locus_tag="VFQ35_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030701 - NAD+-dinitrogen-reductase
                     ADP-D-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HET9929825.1"
                     /translation="SHEFQSHPRALHIVGVRRENRSLFKLLATMEDPIERAQLFDEYM
                     TAKFRLDEWQAQTGVSRRSIRNSYLRFLRGWGFDSNSIEGAVLKGWVESRLGIAPSYH
                     GRRIEPGRQSYLEFASQRVRGQACTNAIDAQLDLLFEFCQYELRRRQLLRITLYRGVQ
                     EADEIHTLARRGDGARLVRLNNLCSFTSEVERAWEFGSTVFQAILPAQRVFYFSGLLP
                     KSILRGEDEYLVVGGECWINLVRC"
     gene            complement(797..1237)
                     /locus_tag="VFQ35_04030"
     CDS             complement(797..1237)
                     /locus_tag="VFQ35_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9929826.1"
                     /translation="MSDELLDFLLANGFDVADIDAPAEHGLTPLMRAALLGRLDMLDA
                     LLERGAKLGARNDDGNNALWLACVSGRTEVVRRLHRAGIDLDNKNETGATALMYTASS
                     GKAELVRLLLELGADPHVQNLDGAKAGDMAATLDCLKLLRHTIS"
     gene            complement(1234..>2356)
                     /locus_tag="VFQ35_04035"
     CDS             complement(1234..>2356)
                     /locus_tag="VFQ35_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9929827.1"
                     /translation="KYGERAFRYCELDIGHALAALGYAARLLGWRLTDASHVGWGALF
                     GALGLDREQDFPKRVRNYTELEEPELLVEVVRDASHPPISAAELARLTASASFAGTPS
                     VIDAHPMYAWPAIHDVAAATRWPSREHSRAPAPEAALQHARSDMPAARLISSRRSAQR
                     FDSRFVMEREAFLRLIAAAAANPACAALFPSPMVDLVLWVHRVEGVEPGVYVLPRTSE
                     RPLLTWLSRKFEVRPVASGEVGVELFRVAAVAPRELSRIARTLCCHQDIASSCAFAVA
                     MVADIERALALDAFAYRLVHREAGAVGQALYLEAEAAGVRGTGIGCFLDDPTAEFLGV
                     ADTPARSVYHFSVGKPIDDPRIETTRAGVLAKANGEEDK"
BASE COUNT          428 a          772 c          762 g          394 t
ORIGIN      
        1 cttcgcacga gttccagagc cacccgcgtg cgttgcacat cgtcggcgtg cgtcgcgaga
       61 accgctcgct tttcaagcta ctcgccacga tggaagatcc catcgagcga gcgcagctct
      121 tcgacgagta catgaccgcg aaattccggc tcgacgagtg gcaggcgcaa accggcgtga
      181 gccgccgcag cattcgcaac agctacctca ggttcctgcg tgggtggggg ttcgattcga
      241 actccatcga aggcgccgtg ctgaaaggct gggtagagag tcgtctcggt atcgcgccgt
      301 cctatcacgg ccgacgcatc gaacccgggc gacagagcta cctcgagttc gcatctcagc
      361 gcgtgcgcgg acaggcgtgc acgaacgcca tcgacgccca gctcgatctg ctattcgagt
      421 tctgtcagta cgagctccgg cgccgtcaac tgctgcgcat cacgttgtac cgcggagtgc
      481 aggaagccga cgagatccat accctggctc ggcggggtga cggcgcgcga ctcgtccgat
      541 tgaacaatct gtgttcgttc acgagcgagg tcgaacgggc ttgggaattt ggttccacgg
      601 tgttccaggc gatcttgccg gcgcagcgcg tcttctactt ctctgggctc ctgccgaaga
      661 gcatcttgcg gggcgaagac gagtacctgg ttgtcggcgg ggagtgctgg atcaacttgg
      721 tgcggtgctg aacgcgagcg attcgaggct accagcttca acgcaacctc gagaccctgc
      781 ctcgttgcgc ggtcgctcac gagatcgtgt gccggagcaa cttcaagcag tcgagcgtcg
      841 cggccatgtc gcccgccttc gccccgtcga ggttttggac gtgcgggtcg gcgccgagct
      901 cgagcaagag ccgaacgagc tccgccttgc cactcgaggc ggtgtacatc agcgcggtgg
      961 cgccggtctc gttcttgttg tcgagatcga tgccagcgcg gtgcaaacga cgcacgacct
     1021 cggtgcgccc ggagacacaa gcgagccaca gcgcgttgtt gccgtcgtcg ttccgagcgc
     1081 cgagctttgc tcctcgctcc aagagcgcgt ctagcatgtc caaacggccg agcagcgccg
     1141 cgcgcatcaa cggagtcagg ccatgctcgg cgggggcgtc gatgtcggcg acgtcgaagc
     1201 cgttagccaa aagaaagtcc aagagttcgt cgctcatttg tcctcctcgc cgtttgcctt
     1261 cgcaagaact ccagcgcgcg tcgtttcgat ccggggatcg tcgatgggtt tgccgacgct
     1321 gaagtgataa accgagcgcg ccggcgtgtc ggccaccccg aggaactccg cggtgggatc
     1381 gtccaagaag caaccgatcc cggtgcctcg aacacccgcc gcttccgcct cgaggtagag
     1441 agcctggccc acggctcccg cctcgcgatg cacgagccga tacgcgaacg catcgagcgc
     1501 caacgcacgc tcgatgtcgg ccaccattgc caccgcgaag gcacagctcg acgcgatgtc
     1561 ttgatggcag cacaaggtcc gcgcaatgcg cgaaagctca cgcggggcca ccgcggcgac
     1621 tcgaaagagc tcgactccga cttcgccgct cgcgaccggg cgaacctcga acttgcgcga
     1681 gagccaagtg agcaacggcc gctcgctggt tctcggcagt acgtacacac cgggctccac
     1741 cccctcgacc cgatgcaccc acaggaccaa gtcgaccatg gggctcggaa agagtgctgc
     1801 acacgccggg ttggcagcgg cggcagcgat gagacgcagg aacgcttcgc gctccattac
     1861 gaagcgcgag tcgaagcgtt gcgcgctcct gcgcgacgag atgaggcgcg ctgcaggcat
     1921 gtcgctgcgc gcgtgctgaa gagccgcctc gggcgccggc gctctcgaat gctccctgga
     1981 aggccagcgc gtcgccgcgg cgacgtcatg aatcgctggc cacgcgtaca tcgggtgagc
     2041 gtcgatgacg ctgggagtgc cggcaaagct cgcagaagcc gtcaggcgcg cgagctccgc
     2101 cgcggaaatg ggagggtggc tcgcgtcgcg caccacctcg acgaggagct ccggctcttc
     2161 gagctcggtg tagttgcgga cgcgcttcgg gaagtcctgt tcccggtcca aaccgagcgc
     2221 gccgaagagc gctccccaac cgacgtgcga agcatccgtg aggcgccacc ccagtaagcg
     2281 cgcggcatag ccgagggcgg cgagcgcgtg tccgatatct agctcgcaat accgaaacgc
     2341 gcgttcacca tacttc
//
LOCUS       DASTKS010000192         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_476392, whole genome shotgun sequence.
ACCESSION   DASTKS010000192 DASTKS010000000
VERSION     DASTKS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476392"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VFQ35_04040"
     CDS             <1..1701
                     /locus_tag="VFQ35_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9929828.1"
                     /translation="VELTTSRLLQFARKLQHAATFRDLLNVAGSEATEALGFAHVWFM
                     VADTEDAQELRLIQVAGERSDVVWDVAPTLKVKGDRFLEALMSADEPVVIYDARVDPR
                     TDKAIVEKLQNRTLINIPLRLLEKPLGLFGLGTFGDEGCRQVTDAELGYLVGMASQIA
                     VAASRIRFLESQAYAERERRELERRLLQIQKLESLGMLAGGIAHDFNNLLTVIMASAG
                     LAQQLSNTEDLSAEIDAVLGAAKRASDLTRQLLAMSREQELSLRPLDMNGQLSQLLDL
                     ARRVLPENISIDLIEGHGLPLVEGDSSQIDQVFMNLFVNARDAMAEGGRLTVETEQVL
                     INGRYTEAHPWARAGRYVLVTVTDTGTGMPREVLDRVFEPFFTTKGTRKGTGLGLAVA
                     YGIVRQHRGMLHCYSEVGVGTTFKIYLPAVERLASEVGTKLQAMPVLGEERILVAEDD
                     ELVRGVAVRILERAGYRVVAVEDGDAACRAISRAEFDLVLLDVVMPGMPCRDVIERIL
                     TFDPRARILLSSGYTAGANVAALTTRTGFELLRKPYDPDQMLIAVRRTLDSNRPPPAK
                     "
     gene            complement(1768..2493)
                     /locus_tag="VFQ35_04045"
     CDS             complement(1768..2493)
                     /locus_tag="VFQ35_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929829.1"
                     /translation="MSAAIHAKAGIARLGAQVVVHGRDLHAECRELGFVHYLLFCVTG
                     RTFEPSVARVLEQAWVCTGYPDARIWCNRIAGYLGSARVDPGLSMSAAIAASNSVAYG
                     FKALSLAYRVQREIPLDLGERGEWLRGALGARRVLHGYGRPQHGHDERIVVALESLAE
                     AGLRAGPQLARCFWLHEELQREKGIEMNVAALWAALAIDFGIEQREYEQFMLLMFTPG
                     YAAVYADQRERPPFAFLAGHQTR"
     gene            complement(2490..3131)
                     /locus_tag="VFQ35_04050"
     CDS             complement(2490..3131)
                     /locus_tag="VFQ35_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929830.1"
                     /translation="MSCVCRAALANELAAEALFDEGVEAARNACVAGATLAAAAITAS
                     AWYDNPLTGARALRDCLEKLGALPLSVDAWRAAFAPGVHPETGDAFAPGFGYVEPRQE
                     SWLLASASRLAECPRAEKLRFYLSHCHPMPSSRLNVAGLAALVYCDHGLSLDDAEREF
                     LLCRVDVALREAARARTLGIARFPFFSDAYFYEGASPPARTRSLSEAMRRVGL"
     gene            complement(3128..>4355)
                     /locus_tag="VFQ35_04055"
     CDS             complement(3128..>4355)
                     /locus_tag="VFQ35_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9929831.1"
                     /translation="QLAHKLLRAISMAFTAPRGPVHLSIASDVLAGKPVAFPDLDLGR
                     QMPAPVDQQALLGMIEALLVANAPVFYVGDDAGADAQRALLLAEGLGARVVASPAGKR
                     WLSHTHRAYRGVLGFSGNSEAARVIGDADLIVAFGATFDELSTNAWSTLPARVPLFSV
                     DGHANHAYRLPHAVPVLASVGRALDAIAECLPRSGPRPVLSLPPPSPVRSTEGGPVHP
                     ADLMRWLSHELPADVVVHVDAGNSFSWSTRDLTRARPDTYRVAMGASSMCWAIAASVG
                     AAIADPRRTICIVGDGSMLMSSLELTVAVEHKLPVTYVVLNDSSLGMVRHGQKLAGAE
                     PIAHDIPVVRFDRLARACGARGMRISELSQLGRVPRAWLASDKGGPALIDVCIDRSAV
                     PPMADRVRGLAVGVPK"
BASE COUNT          783 a         1487 c         1443 g          642 t
ORIGIN      
        1 gttgagctca cgacgagtcg cctgctccaa ttcgcgcgca agctccaaca cgcggctacg
       61 ttcagggatc tcctcaacgt tgcggggagc gaggcgaccg aggcgctcgg cttcgcgcac
      121 gtttggttca tggtggcgga caccgaagac gcgcaggagc tccgcctcat tcaggtagcc
      181 ggagagcgca gcgacgtggt ctgggacgta gcgcccacgc tgaaggtcaa gggcgaccgc
      241 ttcctcgaag cgctgatgtc agcagatgaa ccggtcgtga tctacgacgc gcgcgtggat
      301 ccgcgcaccg acaaggcgat cgtcgagaag cttcagaatc gcactctgat caacattccg
      361 ctgcgcctct tggaaaagcc gctcggcctg ttcggtctcg gcacgttcgg cgacgagggc
      421 tgccggcaag tgaccgatgc cgagctcggt tacctggtgg gcatggccag ccaaatcgcc
      481 gtggccgcca gccggattcg cttcttggag agtcaggcct acgccgagcg ggagcgccgc
      541 gaactcgagc gacgcctgct ccagatccaa aagctcgaga gcctcggcat gttggcgggc
      601 ggcatcgcgc acgacttcaa caacctgctc accgtgatca tggcgagcgc ggggcttgcc
      661 caacaattga gcaatacaga ggacctctcg gcggagatcg acgcggtgct cggcgcggcc
      721 aagcgagcga gcgatctgac ccggcagctc ctcgccatga gccgcgagca ggagctctcg
      781 ctccggccgc tcgacatgaa cgggcagctc tcgcagttgc tcgacctggc gcgccgcgtg
      841 ttgcccgaaa acatcagcat cgacctcatc gaaggccatg gcttgcccct cgtcgagggc
      901 gattcgtccc agatagatca ggttttcatg aacctgttcg tcaacgcgcg cgacgccatg
      961 gcggaagggg gccgactcac ggtcgagacc gaacaggtcc tcatcaacgg acgctacacc
     1021 gaggcgcacc cctgggcgcg cgccgggcgc tacgtgctcg tcaccgtaac cgacaccggc
     1081 accggcatgc ctcgggaggt tctcgaccgg gttttcgagc cgttcttcac gaccaaagga
     1141 acccgcaagg gcaccggact cgggctcgca gtggcctacg gcatcgtgcg ccaacaccgc
     1201 ggcatgctgc actgctacag cgaggtcggc gtaggcacca cgttcaaaat ctacttgcct
     1261 gcggtcgaac gcctcgcaag cgaggtaggc acgaagctcc aagcaatgcc cgtgctgggg
     1321 gaagaacgca tcctcgtggc cgaagacgac gagctcgtgc gcggcgtcgc agtgcgtatt
     1381 ctggagcgag ccgggtatcg cgtggtcgcg gtcgaagacg gggacgcggc gtgccgcgcc
     1441 atcagccgcg ccgagttcga cctcgtgctg ctcgacgtcg tcatgccagg aatgccttgc
     1501 cgcgacgtga tcgagcgcat cctcacgttc gacccacgcg cgcgcattct gctttcgagt
     1561 ggctacacgg cgggcgcaaa cgtcgccgcg ctcacgacac gcaccggttt cgagctgctg
     1621 cgaaagccat atgatccgga ccagatgctc atcgcggtgc gccgaacact cgatagcaac
     1681 cgcccaccgc cggcgaagta gcgacgaaac gttcgaggcg ccacgcgcac acagcgaaga
     1741 cgtttcgtcg cggtggcgcg ccaagcgtca gcgggtttgg tgaccggcga ggaacgcgaa
     1801 cggcggccgt tcccgttgat ccgcgtagac cgcggcataa cccggcgtga acatgagcaa
     1861 catgaactgc tcgtactcgc gctgctcgat gccgaagtcg atcgcgagcg ccgcccacaa
     1921 cgcggccacg ttcatctcga tgcccttctc gcgctggagc tcctcgtgga gccagaagca
     1981 gcgcgcgagc tgcggtcctg ctcgcaggcc tgcctcggcc aggctctcca gcgcgaccac
     2041 gatgcgctcg tcatgtccgt gctgggggcg tccgtagccg tgcagcactc ggcgcgcgcc
     2101 gagcgcgccc cgaagccact ccccccgttc cccgaggtcc aaggggatct cacgttgcac
     2161 gcggtaagct agcgagagcg ctttgaagcc gtaggcgacg ctgttgctcg cggcgatcgc
     2221 ggcgctcatc gaaagccccg ggtcgacccg cgccgagccc aggtagccgg cgattcgatt
     2281 gcaccagatc ctcgcgtcgg ggtaacccgt gcacacccag gcctgctcga gcactcgcgc
     2341 aacgctcggc tcgaatgtcc ggcccgtcac gcaaaacaaa aggtagtgga cgaagccgag
     2401 ctctcgacac tcggcatgca ggtctcggcc gtgtacgacg acttgcgcgc cgagcctcgc
     2461 aatcccggcc ttcgcgtgga tcgcggcgct cacaacccta ccctccgcat cgcctcggac
     2521 aagctgcgcg tgcgtgctgg cggcgacgca ccttcgtaaa agtatgcgtc actgaagaag
     2581 ggaaagcggg cgatgccgag cgttcgagca cgcgccgcct cgcgcaatgc aacgtcaacg
     2641 cggcagagca agaactcccg ctcggcgtcg tccaagctca gcccgtgatc gcagtagacc
     2701 agcgccgcga gcccggcgac gttcaagcgc gagctcggca tcgggtggca atgcgaaaga
     2761 taaaaccgca gcttttctgc gcgggggcac tcggcgaggc gactcgccga cgccagcaac
     2821 caggactcct gtcgaggttc gacgtagccg aagcccggcg caaacgcgtc gcccgtctcc
     2881 ggatggacgc ccggcgcgaa cgcggcccgc cacgcgtcga ccgagagcgg cagcgctccg
     2941 agcttctcca agcagtcgcg cagcgcacgg gcgccggtca gcggattgtc gtaccacgcg
     3001 ctcgcggtga tggcggccgc ggcgagcgtc gctcccgcca cgcaggcgtt ccgcgcagct
     3061 tcgaccccct cgtcgaagag tgcctcggcg gcgagctcgt tcgcgagtgc ggcgcggcaa
     3121 acgcagctca cttgggtacc cccaccgcga gcccgcgcac gcggtcagcc atgggaggca
     3181 cggcgctccg gtcgatgcaa acgtcgatta gcgcggggcc gcctttgtcg ctcgcgagcc
     3241 aggcgcgcgg cacacgaccg agctgggaaa gctcgctgat tcgcatcccg cgagccccgc
     3301 acgcccgcgc cagccggtcg aagcgcacca ccgggatgtc gtgcgcaatg ggctcggcgc
     3361 cggccagctt ctgtccgtgg cgcaccatgc cgaggctcga atcgttcaac acgacatagg
     3421 tgacgggcag cttgtgctcc accgccacgg tgagctcgag gctcgacatc aacatcgacc
     3481 cgtcgcccac gatgcagatg gtgcgacgcg ggtcggcgat ggcggctccc accgacgccg
     3541 cgattgccca gcacatgctc gaggcgccca tcgcaacgcg gtacgtgtcc ggccgtgcgc
     3601 gcgtcaaatc ccgcgtcgac cacgagaagc tgttcccggc atcgacgtgc acgaccacgt
     3661 cggcgggcag ctcgtgactg agccagcgca tcaagtcggc agggtgaacc ggccctccct
     3721 cggtgctgcg aaccggtgac ggaggaggca acgaaagcac cggacgaggg ccgctgcgcg
     3781 gcaagcactc cgcgatggcg tcgagcgcgc gcccgaccga cgcgagtacc gggacggcgt
     3841 gcggcagacg gtaagcgtgg ttcgcatggc cgtcgacgga gaagagcggg acgcgagccg
     3901 gcaacgtgga ccaagcattc gtcgagagct cgtcgaaggt tgcgccaaag gcgacgatga
     3961 gatcggcgtc tccgatcacc cgtgcggcct ccgagttccc ggagaagccg agtacgccgc
     4021 gataggcgcg gtgcgtgtgc gaaagccagc gcttacccgc ggggctcgcc acgacgcgcg
     4081 ccccgagtcc ctccgcgagc aacaaggccc gctgagcgtc ggcgcctgcg tcgtcaccca
     4141 cgtagaatac cggcgcgttc gcgacgagca gcgcttcgat catgccgagc agtgcttgct
     4201 ggtcgacagg cgctggcatt tgtcggccga ggtcgaggtc cgggaaggcg actggctttc
     4261 cggcgagcac gtcggaggca atcgacaagt gcaccggccc gcgcggcgcc gtgaatgcca
     4321 tgctgatggc gcggagaagc ttgtgcgcga gctgc
//
LOCUS       DASTKS010000193         5434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_251608, whole genome shotgun sequence.
ACCESSION   DASTKS010000193 DASTKS010000000
VERSION     DASTKS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251608"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFQ35_04060"
     CDS             complement(<1..321)
                     /locus_tag="VFQ35_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929832.1"
                     /translation="MKLRRLGFSGRGFWSVSLALLTSVGCVARSPSGAAAPTANNAVT
                     DAPSGPEGLTAPEPLGAAAARTTGKYRNVFAERGKPQAEIDEKIKRAYETYFSTTDPN
                     RLYWE"
     gene            complement(383..1663)
                     /locus_tag="VFQ35_04065"
     CDS             complement(383..1663)
                     /locus_tag="VFQ35_04065"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HET9929833.1"
                     /translation="MESSSITTLSDIRAAANRIRGNVRVTPCVESGRLSALTGTNLFL
                     KFENLQETGSFKERGAANLLELLSAEERARGVVTASAGNHAQAVACHAQRLGIAAYVV
                     MPETTPLVKIMSTRRFGAHVELAGKGYDEAAERAAAIGAERNLVYVHPFDDARVIAGQ
                     GTLGLELLEQQPDLEAIVVPVGGGGLIAGIACAVKETAPHVAVYGVESVAFPGMKRHL
                     DTDAPPSLRGGKTIADGIAVRHVGALTTPLVRKYVDDVVLVDEEDIAEAILVLLEQEK
                     TVVEGAGAVGFAALLQGALPVINKKVAVLLSGGNIDVNLVSRIIERGLVKTGRLTRIE
                     LTVADVAGTLARIADAIGATRANILEIQHDRTFTDAELGETRMELVLETFGFDHVEKV
                     REGLERAGYQPTVLAGPPSLGSAPPVSVRPPRRP"
     gene            1683..2099
                     /locus_tag="VFQ35_04070"
     CDS             1683..2099
                     /locus_tag="VFQ35_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET9929834.1"
                     /translation="MALETLVLSLHVVANLFWIGSIVAVGLLVSAPKPDLPVRGGLAR
                     LIYRRIAAPAFAVSFVTALLRLALSPSYYFVATHFMHAKILLGLGVIGLHHVLGARAR
                     RAERGEASAMGGVPALTAALAVLAAGTAWLAIAKPF"
     gene            2468..4963
                     /locus_tag="VFQ35_04075"
     CDS             2468..4963
                     /locus_tag="VFQ35_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929835.1"
                     /translation="MPIDREKVLQAAQKYVEKKRYDGAIAEYQKIIQEDPNDARTLLK
                     IGDLQARLQAYPEAIATYDRVGQYYATQGFSLKAIAVYKQIREIIKKHAPDLGDRYAH
                     IGPRLAEIYTQLGLTSDALSAWDEVATRMQRAGRDREAVDIFRKMVELDANNPLPHLR
                     LAEACCRVHALDDAIESFWTAADLLLNLGRQEDALKVIERILHFRSDPRFARVAAELY
                     LGRATREDGLQALAKLQICFQADPKDLDTLGLLGQAFTLIGQADKAIEVYKEMARVAR
                     DQGRGDVYDGLVDHLRSVAPDDDQVRAFESFLPPNAPIESVPAQAVDDDVELIEAESV
                     RTEVEEIHSSPDLLVVDDTFEAVEEIPASSAFDASAHARKAIVDAESFRKLRLFPKAI
                     ETLHIALEIDPRSLEIRGKLREILLESGDGSGALAESVNLAALHIDTGDLASAEPLLR
                     QVLEEQPEHEGAHALWEQLGKGPLVRRSSPSNAYQSYEQYDNDPSAPLPSYDLEEISA
                     DQAMLHQGRHEEFTEDPLPSFPLAGETTDTAMVEDLDDVTDIEELPPVDTDALEQALE
                     EAEFFVVRGLYEDARAILEEQLVRTPNHPLVYERLHEIDEHLGRRSGSQTIERSSLGA
                     VAPKSLDVSASLDALDALELAPNAHPEPAPAPSAKVDADKIFAKFKAGIRAQVSENDS
                     ATHYDLGVAYKEMGLIADAVHELEVAARDSERECMCHAMIGLIHLEQGALDQAAESYI
                     KALAAPTKTVEQEMNLYYDLGNVYEMKSKAQDALYYFQKIARRDPGYRDVADRIAALL
                     QEKPISEPSGTRAVNDDDEFERVFDDLFESKSK"
     gene            4976..5299
                     /locus_tag="VFQ35_04080"
     CDS             4976..5299
                     /locus_tag="VFQ35_04080"
                     /inference="COORDINATES: protein motif:HMM:NF039368.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HET9929836.1"
                     /translation="MLKLAFRRIKPECEERLRRWFAELESRRAEVLETLVGERVRHEQ
                     AFVLETSDGPVLVWAAELDDPEHAAATFAQSPHAIDREHARVLRECLDQTPPVTRVFD
                     VAVAP"
BASE COUNT          942 a         1882 c         1700 g          910 t
ORIGIN      
        1 ctcccagtac aaccgattgg gatccgtggt cgagaagtag gtctcgtaag cgcgcttgat
       61 cttctcgtcg atctccgctt gcggctttcc gcgctctgcg aacacgttgc gatacttacc
      121 cgtcgtgcgg gcggcggccg ccccgagcgg ctctggcgcg gtgagccctt ccgggccaga
      181 cggggcgtcg gtcacggcgt tgtttgccgt cggcgccgcg gctccgctgg gagagcgcgc
      241 gacacaaccc accgaagtga gcagcgcgag cgaaaccgac caaaaccccc gtcccgaaaa
      301 cccgagtcta cgaagcttca tgaggcactc cagctgattg gcgcgcggac gatagcacgc
      361 tcgagtcgcc cggagcttcg gttcacgggc gtcgcggcgg ccgaaccgat actggcggag
      421 cgctgccgag tgagggcggg cccgccagca ccgtgggctg gtacccggcg cgttcgaggc
      481 cctctcgtac cttctccacg tggtcgaagc cgaacgtctc gagcacgagt tccatccgcg
      541 tctcgccgag ctcggcgtcg gtgaaggttc ggtcgtgctg aatttcgagg atgttggctc
      601 gggttgcgcc gatcgcgtcc gcgatgcgcg ccaaggtgcc cgcgacgtcc gcaaccgtga
      661 gctcgatgcg cgtgagccgt ccggtcttga cgagaccgcg ttcgatgatg cgcgatacga
      721 ggttgacgtc gatgttgccg ccgctcagca gcaccgcgac cttcttgttg atgaccggca
      781 gcgcgccttg caggagcgcc gcgaagccga ccgcgcccgc tccttcgacg accgtcttct
      841 cttgttcgag caagaccagg atcgcctctg cgatgtcttc ttcgtccacc aacacgacgt
      901 cgtctacgta cttgcggacg agcggcgtcg tgagcgcgcc gacgtgccgt accgcgatgc
      961 catcggcgat ggtcttgccg ccgcgcagcg acggtggcgc gtcggtgtcc aggtggcgct
     1021 tcatccccgg aaaagcgacc gactcgacgc cgtacaccgc gacgtgcggc gccgtctctt
     1081 tcacggcgca cgcgatgccg gcgatcaagc cgccgccgcc gaccggcacc acgatcgctt
     1141 cgaggtcggg ctgttgctcc aagagctcga gcccgagcgt gccctggcca gcgatgacgc
     1201 gcgcgtcgtc gaacgggtgc acgtacacga gatttcgctc cgcgccgatc gcggccgcgc
     1261 gctcggcggc ttcgtcgtaa cccttcccgg cgagctcgac gtgcgctcca aagcgccgcg
     1321 tactcatgat cttcacgagc ggcgtcgtct ccggcatcac gacgtaggcc gcgatgccga
     1381 ggcgctgcgc gtgacaagcg accgcctgcg cgtggttacc ggcgctcgcc gtcaccacgc
     1441 cgcgcgcgcg ctcctcggcg ctcaaaagct cgagcaagtt cgcggccccg cgctctttga
     1501 aagagccggt ctcctgcagg ttctcgaatt tcaggaagag gttcgtgccc gtcagcgccg
     1561 agagccgccc gctctccacg cagggggtta cgcgcacgtt acctcggatg cgattcgccg
     1621 ccgctcggat gtccgacaac gtggtgattg acgacgactc cattccgaag aatgtacacc
     1681 gcgtggcgct agaaacactg gtgttgagtt tgcacgtcgt ggcaaacctg ttttggatcg
     1741 ggtcgatcgt cgcggttggc ctactcgtct ccgctcccaa gccagacctg cccgttcgag
     1801 gtggcctggc tcggctcatc taccgccgca tcgcggcgcc ggcattcgcc gtctcgttcg
     1861 tcacggcgct gctccgcctc gcgctcagcc cgagctacta tttcgtcgcg acgcatttca
     1921 tgcacgcgaa gatcctcctc gggctcgggg tcattggcct tcaccatgtg ctcggcgcac
     1981 gagctcggcg cgccgaaagg ggcgaagctt ccgcgatggg gggcgttccg gccctgaccg
     2041 ccgcgcttgc cgtcctcgcg gccggcaccg cttggctcgc gatcgcaaag ccgttctgag
     2101 ctcgttcggg cccggcgtcg gtccgcagcc gctcggagcg cgcggtttcg ccccgatgtg
     2161 agcgcgcggt gtcacgcgtg ggcaggaaac ggcacatgtc tcgacctgcg cgcggacgtg
     2221 ggggacggac ccagctgtgc ccaggcagga gccgcgggtg ttttgcgcgt ttcagagggg
     2281 tttgcggtgt gccgccgaag gtacgaaatc gatggcacac acgtgtctcc gaaagcgccg
     2341 gtttgtgcag gatttcccga gggttttctt gacggagccc cccctcaccc ctagactttc
     2401 caggcggtga acgcggcgtt ttcaacggca tcgggccgac cgacttgccc gctcacctgg
     2461 cgacacagtg cccatagatc gcgagaaagt tctccaagcc gcccagaagt acgtcgagaa
     2521 gaagaggtac gacggggcga ttgcggagta ccaaaagatc atccaggagg atccgaacga
     2581 tgcgcggacc cttctgaaga tcggcgattt gcaagcgcgt ttgcaggcct accctgaggc
     2641 catcgcgacc tacgatcgcg taggtcagta ctacgcgact caaggttttt cgctgaaggc
     2701 catcgcggtc tacaagcaaa tccgcgagat catcaagaag cacgcgccgg atctcggcga
     2761 tcgctacgcg cacatcgggc cgcgtttggc ggagatttac acgcagctcg gcctcaccag
     2821 cgacgcgctc tccgcctggg acgaggttgc gacgcgcatg cagcgcgccg gccgcgaccg
     2881 tgaagcggtc gacatcttcc gcaagatggt cgagctcgac gcgaacaatc cgctgccgca
     2941 cctgcgcctg gccgaagcgt gttgtcgcgt tcacgccctc gacgacgcga tcgagtcttt
     3001 ttggacggcc gccgatttac tgctgaatct cgggcgacaa gaagacgcgc tcaaggtcat
     3061 cgagcgcatc ctgcacttcc gctccgatcc acgctttgcg cgcgtcgcgg ccgagctcta
     3121 cctcgggcgg gccacgcgcg aagacggcct gcaggcgctc gccaaactcc aaatctgctt
     3181 ccaggcagat ccgaaggatc tcgatacgct cggtttgctc ggtcaagcct tcacgctgat
     3241 aggtcaagcc gacaaagcga tcgaggtcta caaagagatg gcgcgcgtgg cgcgcgacca
     3301 agggcgcggc gacgtgtacg acggcctcgt cgatcacctg cgctcggtcg cccccgacga
     3361 cgatcaggtg cgcgccttcg agagcttcct gccgccgaac gcgccgatcg agtcggtgcc
     3421 ggcgcaggcc gtcgacgacg acgtcgagct catcgaagcc gagtcggtgc gaaccgaggt
     3481 cgaagagatc cacagctcgc ccgatttgct ggtcgtcgac gacacgttcg aggccgtcga
     3541 agagatcccg gcgagcagcg cgttcgacgc gagcgcgcac gcccgcaagg cgatcgtcga
     3601 cgccgagtcg tttcgcaagc tgcgcctctt cccgaaggcc atcgagacgc tgcacatcgc
     3661 gctcgaaatc gatccgcgct cgctcgagat tcgcggcaag ctgcgggaga ttctgctcga
     3721 gtcgggcgat ggctccggtg cgctcgccga gtcggtgaac ctcgccgcgc tccacatcga
     3781 cacgggggat ctcgcctcgg ccgagccgct gcttcgccaa gtcctcgaag agcaacccga
     3841 gcacgaaggc gcgcacgccc tctgggagca gctcggcaaa ggtccgctcg tccgccgctc
     3901 ctcgccctcc aacgcctacc agtcgtacga gcagtacgac aacgacccga gcgctccgct
     3961 cccttcgtac gatctcgagg agatctcggc ggaccaggcg atgctccacc aagggcgaca
     4021 cgaggagttt acggaggatc ccctcccgag cttccccctc gctggcgaaa ccaccgacac
     4081 ggccatggtc gaggacctcg acgatgtcac cgacatcgaa gagctcccgc cggtcgacac
     4141 cgacgccctg gagcaagcgc tcgaagaggc cgagttcttc gtcgttcgcg gcctgtacga
     4201 agacgcgcgc gcgatcctcg aagagcagct cgtgcgcacg ccgaaccacc ccttggttta
     4261 cgagcgccta cacgaaatcg acgagcacct cgggcggcgc agcggcagtc aaaccatcga
     4321 gcgctcgtcg ctcggcgcgg tcgcgcccaa gtccctcgac gtcagcgctt cgctcgacgc
     4381 gctcgatgcg ctcgagctcg cccccaacgc ccaccccgag ccagcgcccg ccccgagcgc
     4441 caaggtcgac gccgacaaga tcttcgcgaa gttcaaggct ggcatccgcg ctcaggtctc
     4501 cgagaacgac agcgcgaccc actacgatct cggcgtcgcc tacaaggaga tgggactcat
     4561 cgccgacgcc gtgcacgagc tcgaggttgc cgcgcgcgac tccgagcgcg agtgcatgtg
     4621 ccacgcgatg atcggcctca tccacctcga gcaaggtgcg ctcgatcaag ccgccgagtc
     4681 gtacatcaaa gccctcgccg cacccaccaa gacggtcgag caagagatga acctctacta
     4741 cgacctcggt aacgtctacg agatgaagag caaggcgcaa gacgcgctct actacttcca
     4801 aaagatcgcc cgccgcgacc ccggctatcg tgacgtcgcc gatcgcatcg cagccctcct
     4861 ccaagaaaag cccatcagcg agccgagcgg cacccgcgcc gtcaacgacg acgacgaatt
     4921 cgagcgcgtc ttcgacgatc tcttcgagag caagagcaaa tagggcccga tcgccttgct
     4981 caaactcgca tttcgccgca tcaaacccga gtgcgaagaa cggcttcggc gatggttcgc
     5041 cgagctcgag tcccgccgcg ccgaggttct agagacgctc gtcggtgagc gagtgcgaca
     5101 cgagcaggcg ttcgtcctcg aaacctcgga cggccccgtt ctcgtgtggg ctgccgagct
     5161 cgacgatccc gaacacgccg ccgccacgtt cgcgcagtcg ccccacgcca tcgatcgcga
     5221 gcacgctcgt gtcctgcgtg agtgcctcga ccaaaccccg cccgtcacgc gcgtattcga
     5281 cgtcgccgtc gcgccgtagc cgaaccctca gcgatcgacg atcgacgcga cgagctcggc
     5341 gctggcggcg agaatgccgt cgacggtggc ttcgccgctg aacttccagg tgttggtgcg
     5401 gtgctgcacg acctcgatgc gtaagtcgag tcga
//
LOCUS       DASTKS010000194         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_251859, whole genome shotgun sequence.
ACCESSION   DASTKS010000194 DASTKS010000000
VERSION     DASTKS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251859"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFQ35_04085"
     CDS             complement(<1..749)
                     /locus_tag="VFQ35_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9929837.1"
                     /translation="MGEFDEIIEEFLIESNEGLDQLDQDLVALEERPNDRELLARIFR
                     CAHTIKGTSGFIGLSKLEALTHAGETLLSKMRDGELSLTPDITTLLLEMVDAIRKMLE
                     SVRAAGNDGESDYAALIELLKLAALGSSGAAQVARANASKKPRASAAPRKVSRAPASA
                     PRAATENASSPVSVPLPSVELSRPVELVELPVAAIELPAVAVAPRSEPSHSAEPEPEV
                     TAEPVEPAPVSLQAQHAAPPASTRGSREASAT"
     gene            complement(805..>2320)
                     /locus_tag="VFQ35_04090"
     CDS             complement(805..>2320)
                     /locus_tag="VFQ35_04090"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET9929838.1"
                     /translation="GFDWRRSTAASLDDPNAPRGRGIALARKLAFDSLDYFGSGNRLA
                     ARVDFDRLVVSTSLVPSMRFDRAVAHDRFVSQDLPEAERAHIQREAQLVQSQKSADQL
                     LHEEALLAAKLLGGRKACIGLLTPDGCLEAFVVDTCGEREVQMFSSADKLPEVWSRVL
                     SLEAYLIINEPRSVAALGLVQQSVLVPIRHRLAVIGFLHVAENGADWTQLDALRLAEL
                     AQHFAPLFAARTVAEVAARQARTAAAEQARALEDQEAARYLVGCLLREGCMAEPGIRH
                     YATSLEVFNGDIALAARTPKGALRWMLGDFVGHGLSAAIGGIPLASIFYATARKGVPL
                     IEVVVTMNQMLRDVLPPGYFCAAILLELGDGQLSYWNGGMPPGLLRSSVNHDVRELPS
                     GDFPLGIVGEESLELSLQTLTVHEGDRVLCYSDGLIEARSPDGILFGVERAIRAYADA
                     DAEDAFEHLSSTLAEFRGVADASDDLSIVEITVGLADRGAPEEWSQAQRSTRGG"
BASE COUNT          404 a          782 c          771 g          363 t
ORIGIN      
        1 gtcgcagaag cttcgcgaga tccccgcgtc gaggcgggcg gcgcggcgtg ctgcgcctgc
       61 agcgaaaccg gcgccggctc taccggttcg gccgtgacct cgggctccgg ctcggcggag
      121 tggctcggct cgcttcgggg cgctaccgca accgcgggca gctcgatcgc cgcgacgggc
      181 agctcgacga gctcgacggg gcgactcaac tccaccgacg gcaacggcac ggacaccgga
      241 ctcgaagcgt tctcggtagc ggcgcgaggc gccgacgctg gcgcgcgcga aacttttcgt
      301 ggcgccgccg atgcgcgagg cttcttcgac gcgttcgcgc gggcgacctg ggccgcgccc
      361 gaagagccga gcgcagccag cttcaggagc tcgatgagcg cagcgtagtc cgactcaccg
      421 tcgttcccgg ccgcgcgaac gctctccagc atcttgcgga tagcgtcgac catctcgagc
      481 aagagggtcg tgatgtccgg ggtgaggctg agctcgccgt cgcgcatctt gctgagcagc
      541 gtctcgcctg cgtgcgtcag cgcttcgagc ttggagaggc cgatgaaccc ggacgtgccc
      601 ttgatggtgt gcgcgcagcg gaagatccgg gcgaggagct cgcgatcgtt cggccgctcc
      661 tcgagcgcga cgagatcctg atcgagttga tcgaggcctt cgttgctctc gatcaagaac
      721 tcttcgatga tttcgtcgaa ttcgcccatc gtcgtctcgt gatacgggac gcctgaattc
      781 gatctttagg gaggcgtgcc agacctaccc acctcgggtg ctccgctggg cctgggacca
      841 ctcctcgggg gcgccgcgat cggcgagccc gaccgtgatc tccacgatcg aaaggtcgtc
      901 cgacgcatcg gcgacaccgc ggaattcagc cagcgtcgac gagaggtgct cgaacgcatc
      961 ttctgcatcg gcgtccgcgt acgctcgaat cgcgcgttcg acaccgaaca gtattccgtc
     1021 cgggctgcga gcttcgatga ggccgtcgga gtaacagagc acgcggtcac cttcgtgcac
     1081 cgtcaatgtc tgcaacgaca gctcgagcga ctcctcgccg acgattccca gcggaaagtc
     1141 gccggatggc agctcgcgaa cgtcgtggtt gacggatgac cgcaagagcc cgggcggcat
     1201 tccgccgttc caataactga gctgcccgtc accgagctcg agtaggatgg cggcacagaa
     1261 gtaccctggc ggcagtacgt cgcgcagcat ctggttcatc gtgacgacca cctcgatgag
     1321 cgggacgccc tttcgcgcgg tcgcgtaaaa gatggaagca agcggaattc cgccgatcgc
     1381 cgcggagagc ccgtgcccca cgaagtcgcc gagcatccag cgcagcgcgc ccttcggggt
     1441 gcgcgcggcg agcgcgatgt ctccgttgaa cacctcgagc gatgtcgcat agtggcggat
     1501 gccaggttcc gccatgcacc cttcgcgcaa caggcacccg accaaatagc gggccgcctc
     1561 ctgatcctcc aaagcgcgcg cctgctcggc cgcggcggtg cgagcctgcc gcgccgcgac
     1621 ctccgctacc gttcgagccg cgaacagagg cgcgaagtgt tgagcgagct cggcgagacg
     1681 cagcgcgtcg agctgcgtcc aatccgcgcc gttttccgcc acgtgcaaga agccgatgac
     1741 cgcgagtcgg tgccggatcg gcaccaacac cgactgctgg acgagcccga gcgccgccac
     1801 ggagcggggc tcgttgatga tcaggtacgc ctccaacgac agcacgcgtg accagacctc
     1861 cggcagcttg tctgcgctcg agaacatctg gacttcgcgc tcgccgcagg tatcgaccac
     1921 gaaggcctcg aggcagccgt caggcgtcaa aagaccgatg caggccttac gtccgccaag
     1981 caacttcgcc gccagcagcg cctcctcgtg taacagctgg tccgccgact tctgcgactg
     2041 gacgagctgc gcctctcgct ggatgtgggc gcgctccgct tcgggcagat cctgggagac
     2101 gaagcgatcg tgcgccaccg cgcggtcgaa gcgcatcgac ggcaccagcg aagtcgatac
     2161 gaccaggcga tcgaagtcga cgcgcgcggc caaccggttt cccgaaccga agtagtcgag
     2221 ggagtcgaag gcgagcttgc gcgcgagagc gatccctcgg ccccgcggag cattggggtc
     2281 gtcgagcgac gccgcagtgg agcgacgcca atcgaagcct
//
LOCUS       DASTKS010000195         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1368647, whole genome shotgun sequence.
ACCESSION   DASTKS010000195 DASTKS010000000
VERSION     DASTKS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368647"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1222
                     /locus_tag="VFQ35_04095"
     CDS             164..1222
                     /locus_tag="VFQ35_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929839.1"
                     /translation="MKQKAYISSRRAATWAAALCAALLLLAGCGWWKGPINASPGLRW
                     WLFSNFGAQRMCPEMLKRSAALRLAPGGSAVGRFFPEQCRTQINEQAQTVTLDFSGTG
                     YAWTPVAGRVGFWAGASVDYRMDFFLDDDAVYVWALTARIAHGPEFKLGSVEYKLANW
                     AAQGPAGYLLNTFGSQLMGSQLANGFTVVHTDSGDEFSLGHLSPPQRPPTPFVKGKDR
                     FTFANEVTEVKSGQVDFLGPFEVVEQGQGLFLRMNVTGPAVDVLVVTRSSGDVWRNSL
                     QLGAALAPPSIPPVASWAVQPGGEQQQMLRVPPGQYYVVVDNSARVGTVNPPWSPLGV
                     VGGSTAVIAYIAELGQVD"
     gene            1283..2323
                     /locus_tag="VFQ35_04100"
     CDS             1283..2323
                     /locus_tag="VFQ35_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929840.1"
                     /translation="MSDDSPKPPTPTRVKPPLPPSARARSPTLSDGQKEQLALRALGL
                     LNEQIEARIVHYKADLETSPELDAITAQVVSQLKAMQAALSQQTEVKISPDEIEAKQT
                     KTLTQLLARVFNKDSPFPTRVLTPVGRRVAKLFFESELHEKTKGDKEKRIFHAEQALY
                     YVLQRYRNRLRNELEGFEYDSTEVREATFELLTKTERDLQVGFLSRRSPELNRVMTIF
                     AGVFSDFVTLHLPPRLEQMAKVTIRSGRTARRPNSVPYKILADAFVDFRHEWERLAVQ
                     QMVFYCGDELLSRLTEDGGEFREETVKFFSDPHVFGESCAVLCSELYEYLCLEGFLDL
                     PVDWRVSLQAAE"
BASE COUNT          426 a          761 c          797 g          440 t
ORIGIN      
        1 aggcgcgcgg ctgggcaaaa gagctcggcc tcgagttcgt ccgggttccc gccttgaata
       61 cggcagcttc gctgatttct gcgctcggtg cggtcgctcg ccgcgcgctt ttgggttgag
      121 accgcgcgct gaactctgcg agcatgcccc catcgtggga ccggtgaaac agaaagctta
      181 catttcgtcc cgtcgcgctg ccacttgggc agccgctctg tgcgccgcgt tgctcctcct
      241 tgcggggtgc ggttggtgga aagggcccat caacgcgagc ccgggcctgc gctggtggtt
      301 gttctccaat ttcggagcac agcggatgtg cccagagatg ctgaagcgca gcgcggcgct
      361 gcggctcgcc ccgggcggga gcgcggtcgg ccgcttcttc ccggagcaat gccgcactca
      421 gatcaacgag caggcgcaaa ccgtgacgct cgacttctcc gggacggggt acgcctggac
      481 gccagtcgcg ggacgcgtcg gattctgggc gggcgcgtcg gtcgactacc gcatggactt
      541 cttcctggac gacgacgcgg tttacgtgtg ggcgctaacc gcgcgcatcg cgcacggccc
      601 cgagttcaag ctgggtagcg tcgagtacaa gctcgcgaac tgggcagcgc agggcccagc
      661 cggctacctg ctcaacacgt tcggctctca gctcatggga agccagctcg cgaatggctt
      721 caccgtggtg cacacggaca gcggcgacga gttttcgctc ggccacttga gtccgccgca
      781 gcgaccgccg acgcctttcg tgaagggcaa agatcgcttc acgttcgcga acgaagtcac
      841 cgaagtaaag agcggacagg tcgattttct cggcccattc gaggtcgtgg agcaggggca
      901 gggcctcttt ctccgcatga acgtgacagg gccggcggtc gacgtgttgg tcgtgacgcg
      961 cagctcgggt gacgtgtggc gcaactcgct gcaactcggc gctgccctcg cgcctcccag
     1021 cattccgccc gtcgcgagct gggccgtgca gcctggggga gagcagcagc aaatgctgcg
     1081 ggtgccgccc gggcagtact acgtcgtggt cgacaacagc gcgcgggtcg gcacggtaaa
     1141 tccgccgtgg agtccgctcg gcgtggtcgg cggcagcact gccgtaatcg cgtacatcgc
     1201 cgaactcggc caggtcgact gagctcctgt acggcgaaag cctacatcga tcgcggcaac
     1261 ggctcccgct acgctcgctc gggtgagcga cgactcgccg aagccaccga ctccgacgcg
     1321 cgtgaagccg cctctaccgc ccagcgcgcg agcgagatcg ccgacgttga gcgacggcca
     1381 gaaggaacag ctggccctgc gagcgctcgg gctcttgaac gagcaaatcg aagcgcgaat
     1441 cgtccactac aaggccgatc tagagaccag tccggagctc gatgccatca cggctcaggt
     1501 cgtctcgcag ctgaaggcga tgcaggccgc gctttcgcag cagaccgagg tgaagatctc
     1561 tccggacgaa atcgaggcca agcagacgaa gactctgact cagctcttgg ctcgcgtgtt
     1621 caacaaggac tcgccgtttc cgacccgtgt gctcaccccg gtcgggcgcc gcgtcgcgaa
     1681 gctcttcttc gaaagcgagc tccacgaaaa gacgaagggc gacaaagaga agcgaatctt
     1741 tcacgcggag caggcgctgt actacgtgct gcagcgctac cgtaaccggt tgcggaacga
     1801 gctcgaaggt ttcgagtacg actcgaccga ggtgcgcgaa gcgactttcg agctcttgac
     1861 gaagaccgag cgcgaccttc aagtcggctt tttgtcgcgc cgctcgcccg agctgaaccg
     1921 ggtcatgacg atcttcgcgg gcgtcttttc cgatttcgtg acgctccacc tgccgccgcg
     1981 cctcgagcag atggccaaag tgaccatccg ctccggaagg accgcgcggc gaccgaacag
     2041 cgtgccttac aagattttgg ccgacgcctt cgtcgacttt cggcacgagt gggagcgcct
     2101 cgccgtacag cagatggtgt tctactgcgg tgacgagctg ctctcgcgcc tcacggagga
     2161 cggcggtgag ttccgagaag agacggtcaa gttcttcagc gatccgcacg tgttcggcga
     2221 gtcctgcgcc gtgttgtgct cggagctgta cgaatatttg tgcctcgaag gcttcttgga
     2281 tttgcccgtc gactggcgcg tgagcttgca agccgcggag tagctgaccg gtcggtggag
     2341 ttgacagctg gggtttccga cgtatcgttc ccgagcgtgt ttccgcccga agacctgaaa
     2401 ttgcggctga aagaggcgtt tcca
//
LOCUS       DASTKS010000196         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1814941, whole genome shotgun sequence.
ACCESSION   DASTKS010000196 DASTKS010000000
VERSION     DASTKS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814941"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..709)
                     /locus_tag="VFQ35_04105"
     CDS             complement(17..709)
                     /locus_tag="VFQ35_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9929841.1"
                     /translation="MNTNIVERSRELLLGVGASPILYLMIALSVVSVAIMVERAIFFV
                     RARVDLESLAGELAARLGRGDVEYARQLVVHSRSPEAAVVVAGLNRIEQGAAAVEEAM
                     SAARVIQKMRLERRLGFLGTLGANAPFVGLLGTVIGIVQAFQSLESAGATGAAGTNGV
                     MGAIAEALVATAIGLIVAIPAVVIFNHFKGLLKSRLGNAEFLTRIVVAQLKQRPTAQA
                     FELEEAAIAAEE"
     gene            1078..>2768
                     /locus_tag="VFQ35_04110"
     CDS             1078..>2768
                     /locus_tag="VFQ35_04110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9929842.1"
                     /translation="MALACVALSAASTTARAQPSDTPSAAPTVTPPKLVQSREPTYPE
                     SKKDSGESASVGLILTIDADGHVSNAEVSRSAGADFDEAALSAARQLVFEPATRDGKA
                     TAARIPFEFSFTYTPSAPPVEPVATPPAVLPKEASAPITAASDEPPAEGGELDVAVAG
                     EKPPREPTLRTLQAEEVSKIPGTNGDALRSVTNMPGVARPAGLDGLIIVRGSAPQDTQ
                     IFVDGTSVPLIYHFGGLSSVIPSEVLERIDFYPGNFGPQYGRGMGGIVDVGVRSPKKD
                     RFHGLLQFDSIDGRFLAEGPIDESTRIMVAGRRSWLDAWLGPVLRQAGVGVTVAPRYY
                     DYQAMVERDLSKKTTLRVFAFGADDRTELTLNQPDASDPALGGSALNRDTFWRVQGRV
                     DTRPTDAIRWTSTVSVGKSRQEQVIGELFVDVEALTLEGRSDVRAKLSPAVTAVGGID
                     IVHTAFDAAVRLPPVDFGSDENPGPLFARPATTLKATGGVVRPGAYAMLELTPLPGLK
                     IMPGTRADYNQDTKQWTMDPRVAVRYDVHRGFPRTTLKGGVGIFHQPPEPYESLEP"
BASE COUNT          504 a          946 c          869 g          449 t
ORIGIN      
        1 cggaggcggc catggcttat tcctctgctg cgattgcagc ttcttcgagt tcgaaggctt
       61 gagcggtggg gcgctgcttg agctgcgcaa cgacgattcg cgtgaggaac tcggcgttac
      121 ccagtcgcga cttgagcaga cccttgaagt ggttgaagat cacgaccgcg gggatcgcga
      181 cgatgaggcc gatggcggtc gcgacgagcg cttccgcgat cgcccccatc acaccgttcg
      241 tgccagcggc gccggtagct cccgcgctct ccaggctctg gaaagcttgc acgatgccaa
      301 tcaccgttcc gagtagaccg acgaacggag cgttcgcacc gagcgtcccc aagaagccga
      361 ggcgccgctc gagccgcatc ttttggatca cacgagcagc tgacatcgcc tcttcgaccg
      421 cggccgcgcc ctgctcgatg cggttcaacc ccgcgacgac gaccgctgcc tctggagagc
      481 gcgagtgcac cacgagctgg cgcgcgtact cgacgtcacc gcgaccgagc cgcgccgcga
      541 gttcgcccgc gagcgactcc aggtcgaccc tcgcgcgaac gaagaatatc gcccgctcga
      601 ccatgatcgc gacgctcacg acgctgagcg cgatcatcaa atacagaatc ggtgaagcac
      661 ccactcctag caaaagctct ctggaccgtt cgacgatgtt cgtattcatg acgtgttctc
      721 ccgaactcgc gcggggccga cggggcctcc gtgagcaagt cgcagtcgcc cttcagcacg
      781 gcgcgtgcca gcccgcgaag cgctagaaat tcggcaattt cgagcaactc gacccccgtc
      841 tggacaggtg tccacgcggc cgcacaccaa gcgtccggga gcgacgcgga cggttggtac
      901 gcgccccgac ggctcgtgac acccccgagg catgaccgcg aggagccgct tcaccgttcg
      961 aaacagcgta tttcggagac tgagctcggc gatcattttc actcacgacc tgcttggcgc
     1021 ggcgtttgct gaagggcgca ccgagacacg atgaactcgt caaagagact tcgattcatg
     1081 gctctcgctt gtgtcgcgct cagcgcggca tcgactactg cgcgggccca acccagtgat
     1141 acaccgagcg cggctcccac cgtcacgccg ccgaagctcg ttcaatctcg agaaccgacg
     1201 taccccgagt cgaaaaagga ttcgggagag agcgcgagcg ttggtctcat cctcaccatc
     1261 gacgccgacg gtcacgtgag caacgcagaa gtgagccgtt cggcaggcgc ggacttcgac
     1321 gaggcggccc tcagcgctgc gcggcagctc gtcttcgagc ccgcgacgcg cgatggtaaa
     1381 gcgactgcgg ctcgcatccc gttcgagttc tctttcacgt acacgccgag cgccccgccg
     1441 gtcgagccgg tggcgacgcc gcctgccgtt ctgccgaaag aagcgtccgc gccgattacc
     1501 gccgcgagcg acgaaccgcc cgccgaaggg ggcgagctcg acgtcgcggt ggcaggagag
     1561 aaaccgccgc gcgagccgac cttgcgcacg ctgcaagccg aagaagtctc gaaaatccca
     1621 ggaacgaacg gcgacgcgct ccgctccgtc accaacatgc ccggcgtcgc gcgcccggcc
     1681 gggctcgacg ggctcatcat cgtgcgcggc tctgcgccac aagacacgca aatcttcgtc
     1741 gacggcacga gcgtcccgct catctatcac ttcggcggtt tgagctcggt gataccgagc
     1801 gaggtgctcg agcgcatcga cttctatccc ggcaacttcg gcccccagta tggccgcggc
     1861 atgggcggca tcgtcgacgt gggggtgcgc tcacccaaga aggatcgctt ccatggcctc
     1921 ttgcagttcg attcgatcga cgggcgattc ttggcggaag gccccatcga cgagtcgact
     1981 cgcatcatgg tggcgggccg gcgttcctgg ctcgacgctt ggctcggccc ggtgctgcgg
     2041 caggccggcg tcggcgtgac ggtggctccg cgctattacg actaccaggc gatggtcgag
     2101 cgcgacctct cgaagaagac taccctgcgc gtcttcgcct tcggcgcgga cgatcgcacc
     2161 gaactcaccc tgaatcagcc cgacgcgagc gatccggcgc tgggcggcag cgcgctgaac
     2221 cgcgacacgt tttggcgagt tcaaggtcga gtcgatacgc gaccgaccga cgcaatccgc
     2281 tggacgagca ctgtttcggt cggcaagtcg cgacaagagc aggtgatcgg agagctattc
     2341 gtcgatgtcg aggcgctcac tctcgaaggt cgctccgacg tccgggccaa gctcagtccc
     2401 gcggtcaccg ccgtcggcgg gatcgacatc gtgcacacgg cgttcgacgc cgccgtccgc
     2461 ttgcctccgg tcgacttcgg ctccgacgag aacccgggac cgctcttcgc gcggcccgcg
     2521 acgacgctca aagccactgg cggcgtcgtg cgcccgggcg cttacgcgat gctggagttg
     2581 acgccgcttc ccggactaaa aatcatgccg ggcactcgcg ccgactacaa tcaagacacg
     2641 aagcagtgga cgatggatcc acgcgtcgcg gtccgttacg acgtgcaccg gggattcccg
     2701 cgcacgacgt tgaagggcgg cgtgggcatc tttcaccagc caccagagcc ttacgaaagc
     2761 ctcgagcc
//
LOCUS       DASTKS010000197         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_252380, whole genome shotgun sequence.
ACCESSION   DASTKS010000197 DASTKS010000000
VERSION     DASTKS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252380"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFQ35_04115"
     CDS             <1..277
                     /locus_tag="VFQ35_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929843.1"
                     /translation="YREPSASQSRLLVISGAAIVAARDEPSSYRPAQRTLARAVPPRF
                     DRKSYDRLRVLSTELKRIARDGGEVQVHWGPARSVSQPALASVLALV"
     gene            296..928
                     /locus_tag="VFQ35_04120"
     CDS             296..928
                     /locus_tag="VFQ35_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929844.1"
                     /translation="MENPSDPDLDRAARVMLLSPVRLSGARGRLLLDGTSEASLARQL
                     RSAEGAPLGEVFSFVSGLYFRGKASYASRFAHASGAPAAAFVMCAGAGLCRLEERVDL
                     ERLRGWARVSIREDNPHFTAPLYRHASELLAQHDESARFVLLGSVATNKYVTPLLDVF
                     GERLWFPREFLGRGDMSRGSLLLRAVKDGRELEYRTVQSLVSAEISVREP"
     gene            complement(933..1901)
                     /locus_tag="VFQ35_04125"
     CDS             complement(933..1901)
                     /locus_tag="VFQ35_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9929845.1"
                     /translation="MSLLSPELSPLLRFLPVEHVGTTERIEPIRAGLSGAGVYAVTTS
                     QGSFVLRVQDRNLDENYFAQQLRVLRRAEQAGVAPRIAHVDEAARAIVSARISGKPLG
                     AALADPAQRNSVLASAVEHLRALHSLDSSGVADRDPMRYADETYRAVRERSGFPVWAS
                     DLETEFGHIRRLLARDPRKVVNHNDVNPGNLLWDGERVWLVDWEVTGLDHPHYDLATL
                     ALFLRLDDDAALGLAAMHDGAPLSPDARVNFRALRKLSGLLCGLTFLSLVQDLSIRPA
                     PGIEHAPTLSEVYQGMQRGEHSLQSPLGQTMMGLALLAMSINLKLE"
     gene            1975..2589
                     /locus_tag="VFQ35_04130"
     CDS             1975..2589
                     /locus_tag="VFQ35_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9929846.1"
                     /translation="MTRRPPTNVAPRWQIVSEEGEPAPGTLGIRLLAGGGFGDGTHPT
                     TALCLQALSALAPRGRAFRLLDFGSGSGILAIAAALHLGARVDAVEISPAAIEHAERN
                     FSANGVSDRIRQLTALPPGVAQYDFVIANILRPVLIANARELTAELAPGAVLVLSGLV
                     STDVPEVRAAYAAAFPGSRGEVYASESWRALVFHNALSNHERYE"
BASE COUNT          442 a          884 c          920 g          520 t
ORIGIN      
        1 gtaccgggaa ccgtcggcgt cgcagtcgcg gctgctggtc atctccggcg ccgccatcgt
       61 cgcggcgcgc gacgagccgt cttcctatcg gcctgcacag cgaacgctcg cgcgcgcggt
      121 cccgccgcgg ttcgaccgta agagctacga ccggctcagg gtgctcagca ccgagttgaa
      181 gcgaatcgcc cgtgacggcg gcgaggttca ggtgcattgg ggccctgctc gctcggtgtc
      241 gcagccagcg ctcgcgagcg tgctcgcgct cgtctagcgc actcggagcg tggacgtgga
      301 aaacccttcc gatcccgatc tggaccgcgc cgctcgtgtc atgttgctct cgccggtgcg
      361 gctgagcggc gctcgcgggc gtttgctgct cgatggcacg agcgaggcta gtttggcgcg
      421 acagctacgt tccgccgaag gcgcgccgct gggggaggtt ttcagcttcg tgagcggcct
      481 ctactttcgc ggaaaggcta gctacgcgag ccggtttgct catgcgagcg gcgctcctgc
      541 cgccgcgttc gtgatgtgcg cgggcgcggg gctgtgccgg ctcgaggagc gcgtcgactt
      601 ggagcgcctg cgcggctggg cgcgggtctc gattcgagaa gacaaccccc actttactgc
      661 gccgctctac cggcacgcga gcgagctctt ggcgcagcac gacgagagcg ctcgcttcgt
      721 gctgctcggg agtgtggcca cgaacaagta cgtgacgccg cttctcgacg tgttcggcga
      781 gcggctttgg tttcctcgtg agtttctcgg tcggggggat atgagtcgag gctcgctgtt
      841 gctccgtgcc gtgaaagacg gccgagagct cgaataccgg accgtgcaga gcctcgtgtc
      901 cgcggagatc agtgtgcgcg agccctgagc tctcactcga gtttcaggtt gatgctcatt
      961 gccagcagcg cgagccccat catggtttgg ccgagcgggc tctgcagcga atgctctccg
     1021 cgttgcattc cctggtagac ctcgctcagg gtcggagcgt gttctattcc gggcgccggt
     1081 cgtatcgaga gatcttgaac gaggctcaag aaagtgagcc cgcacagaag ccccgagagc
     1141 ttcctaagcg cccgaaaatt gacacgagcg tccggcgaaa gcggagctcc gtcgtgcatc
     1201 gcggcgaggc cgagcgcggc gtcgtcgtcg aggcgcagga acaacgcgag cgtcgccaga
     1261 tcgtaatgag gatgatcaag cccggtgact tcccaatcga ccagccaaac gcgttcgcca
     1321 tcccacagta agtttccggg gtttacgtcg ttgtggttca caactttcct cgggtcgcgc
     1381 gcgagaagcc tccggatgtg gccgaattcg gtttccaagt cgcttgccca cacgggaaac
     1441 cccgatcgct cgcgcaccgc tcggtaagtc tcgtccgcgt atcgcatcgg gtcgcgatcg
     1501 gcgactccag acgaatcgag cgaatgcaaa gcgcgcaagt gctcgaccgc ggacgcgagc
     1561 accgaattgc gctgagccgg atctgcgagc gccgccccga gcggcttgcc cgagatgcga
     1621 gccgatacga tcgcgcgcgc ggcttcgtcc acgtgtgcga tgcgcggcgc gacgccggct
     1681 tgctcggcac gccgcagcac gcgaagttgc tgcgcaaagt agttttcgtc gaggttccgg
     1741 tcctgcactc gaagaacgaa cgaaccttgg gacgtcgtga cggcgtaaac gccagcgccc
     1801 gagagcccgg ctctgattgg ttcgattcgt tcggtcgtgc cgacgtgctc tacgggcagg
     1861 aagcggagga gaggcgaaag ttctggcgag agcagcgaca ttcgcctctt ctacccaaaa
     1921 aagcgcgtga aggaacgttc cccagtcgct ggcatggagt tatgacggtg ctcggtgaca
     1981 cgccgcccgc cgacgaacgt cgctccccgc tggcagattg tctccgagga aggggaaccg
     2041 gctcccggga ctctcggcat tcgtctcttg gctgggggtg gtttcggaga tggcacgcac
     2101 ccaaccaccg ccctttgctt gcaggcgctc tccgcgctcg cgccccgtgg aagagccttc
     2161 cggctcctgg acttcggcag cggctccggc attctggcaa tcgcagccgc ccttcatctg
     2221 ggtgcccgag tcgacgcggt cgagatctcc cccgctgcaa tcgagcacgc cgaacgcaac
     2281 ttctccgcta acggggttag cgatcgcatc cgccagctga ccgcgctgcc gcccggtgtt
     2341 gcgcagtacg atttcgtgat cgccaacatt ttgcgcccag tgctcatcgc aaacgcgcgc
     2401 gagctgacgg cggagctcgc tccgggcgcc gtcttggtgc tctctgggtt ggtctcgacc
     2461 gacgttccgg aggtgagggc ggcttacgcg gcggcgttcc cagggagccg cggcgaagtc
     2521 tatgcgagcg aatcgtggcg ggcgctcgtc tttcacaacg ctctaagcaa tcatgagcgt
     2581 tacgagtgag gcgtatttgc ttcgcgaccc gctggcggcg gttgagtcgg gacctagact
     2641 caagccatgt cgagcgaact cacccgcgcc gaagtcgacg cactgccagg tgctacggtc
     2701 ctcgagttcg gtgctagctg gtgtccgatc tgccaagggg ctcgtcccct gatcgattcc
     2761 gcgctc
//
LOCUS       DASTKS010000198         7811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1145579, whole genome shotgun sequence.
ACCESSION   DASTKS010000198 DASTKS010000000
VERSION     DASTKS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145579"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFQ35_04135"
     CDS             <1..706
                     /locus_tag="VFQ35_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929847.1"
                     /translation="NPPVQRFEAASAPSPKAAKTPDKIPDTPVAKADAAAPPPDAEVA
                     KKVDQTLLEAPDSAAPTVEGEGSPDGVKEGTETDPLKARAISAYQAKLIGWFNARFRP
                     PESGIPCAELKKLSSGVTVSVGGDRHVAGFSVGSPSGNAIFDERVRTTLERVVGEELP
                     PPPPLYPDILGSTLSFRLSGSKASCDDKPATPSPSPPATPAPEGPAAPEAPAPPPSPP
                     PAPPPAPESPAPDSPP"
     gene            787..2001
                     /locus_tag="VFQ35_04140"
     CDS             787..2001
                     /locus_tag="VFQ35_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolB protein"
                     /protein_id="HET9929848.1"
                     /translation="MLVTGTTQERLPRIAVLPSLSPDIEDVVVRGVVRRDIELTGLFD
                     VIPDSKAPMGLYGFDDPIDVDAWKALGAEAIVKVAARADNSPGKIQVLGIAYFLNVGK
                     DPVYSKKIVVSKNDARVTAHRVTDALLGALTGRPGSFASELTFSARWAKNRRIFKVDA
                     DGNALTPLTVENQHAIAPTWGPGHAIYYLLSENYGPYGLYQWNAGINKPVSVPFKTSL
                     YGVAFDKTNTKMALAVSENATSGIYLGNVDGSGMQRISNTDLATHPVFSPSGKVAWVG
                     GDAKQGSQRIFVNGKAVSPAGFTAASPAFCDTEDGVRLVYSVAVGNDKQDLVISSEDG
                     RGIGRLTQNQGSNTSPACSPDGRLLAFFSTRNKQPGIYLMSLKRYRTQQISSQLGEAL
                     RWAALPPTPGAP"
     gene            complement(2026..2613)
                     /locus_tag="VFQ35_04145"
     CDS             complement(2026..2613)
                     /locus_tag="VFQ35_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9929849.1"
                     /translation="MSSVPNEPAEFFGRWLPEAFEPLAARIGAKNSPGALVFQVGEDA
                     PLAIRIEAGRLSVAEGVPSDTIAQVRLTAGAFEPIIVRGAELLSAEQKSSETHLLVLR
                     ALTLDAERVGIIRGVPGSVAFVLKSDAEEHRLLLTPGTQQPNVQAPECSVRCQLDDFL
                     AMQRGETNPFELMMNGKIQISGDAQIPMALSSLFV"
     gene            complement(2623..2949)
                     /locus_tag="VFQ35_04150"
     CDS             complement(2623..2949)
                     /locus_tag="VFQ35_04150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9929850.1"
                     /translation="MSQRTNKIVVEKRGHPRTAIATVVTCHRKDGTSFEGRSKDISIG
                     GMYLESNEPVAFGAELTIEVTLPRTKGSLKLPAVVRWTDKAGFGVQFGLLGARETHAI
                     SLLIRS"
     gene            3020..3916
                     /locus_tag="VFQ35_04155"
     CDS             3020..3916
                     /locus_tag="VFQ35_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929851.1"
                     /translation="MGPGSSSAGWRRHLEGWELGLLAVVFAMLAVWLALPRPVEPRLV
                     PLPEVDRRVLARSVALDVERAVRAHERPLAYEVRALGEFVRRHGAAVARHDTGAATEA
                     RDEARRLCRSLAARGLFEELQVLRATQTRLFLDAVERFRQGEKDDRELSELGANFVER
                     GQRSGWLDADGRVLLSTEELRALYRMRWAELTGALEIGRLRPSLDEFRLYYRVLLEHP
                     EGLSPPEQDERRLAYVTALARIDSEFPTDLARGVLLARLGRVRSAYDAFSAFVVSHEQ
                     GPWVLRARNYALWALAQASPTE"
     gene            3930..7535
                     /gene="mfd"
                     /locus_tag="VFQ35_04160"
     CDS             3930..7535
                     /gene="mfd"
                     /locus_tag="VFQ35_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9929852.1"
                     /translation="MSEAEPLDDPLAALSLTSDRRLVTLRALATDVLAARRVHHVVDA
                     QGAAPALAAHALALSQSRQVLYVAADADAARRAQSDLAGLSQGLPLPNALGAPSGSEG
                     SLLLATSENTPYAEVHPDRRTAMQRAAGLFRIAAGLPFRFVVVTAAALVRRVVHPAEV
                     RKASVLVEVQADFDVERTSRILVNAGYLRVPVVEDPGTFAVRGGLVDIWPSGAELPLR
                     IELYGDMVSTLRQFDPDDQRTLESVERAWLPPAREAWIDEEQEQRARLALRALCDEVD
                     FPSSKARAFIDDVASGHAYFGLEGYLPAFHELVPLSEYFSHDLPIVVEDPPSVVRALR
                     EELERGRAGAAARSGAPHFAFERLYLEEDELARTLSDRSVLCVHRVSVQGTSEPATMV
                     EALEAVPEGAPALSIRDHEDLARASKTARTGKGKAASLDPLLKRLAIWRESGLSVTIA
                     ARTRTQAERLGALLGHRGIGVRAPESTEAPGPGSVSLVVAPSARGAIAPAEGFVLITE
                     EEIFGQRAHRPAAKPRATKALLEDLRALAPGDYVVHVDHGIGRYLGLEKKDVGGVNVD
                     LIVVEYAGGKLYLPVYRLNQIEKYAASDTTPKLDRLGGQTFAKTKARVEQRVRQMADE
                     LLRLYAERNALTKLPLPAPDDDYAAFEASFPYDETRDQAAAITDVIGDLTAERVMDRL
                     VCGDVGFGKTEVALRAAFLCANAGRQVALLCPTTVLAQQHYMTFSSRLEDYPITVRVM
                     SRFQSKSEHVATLKGLKEGSVDIVVGTHRLLSKDVHFKNLGLLVVDEEQRFGVAHKER
                     IKQLRNAIDVLTLSATPIPRTLQLAVGGLRNMSIITTAPVDRRAIRTITSQFDPELVR
                     TAVERELERGGQVFYVYNRVEGIYERAERLQQLVPRARIAVGHGQLSEEALERTMLNF
                     VAGEYDILVATAIVESGLDIPRANTIIVDRADLFGLSQLYQLRGRVGRSSERAYCYLL
                     VPPPSQMTDEARWRLEALERHTELGSGFHIAALDMELRGAGDVLGADQSGFVASVGFD
                     LFCHMLEEATHELRGETVVHEVDPELSVDVEALLPESYVADVGVRLSLYKRLASATED
                     GEVSDIANELNDRFGEPPIEARRFIELMRLKTELRRLRVLGCEASARSVTLHLRDDTP
                     LDPQKISALIARKKSPYRLSPDGRLTRRALENEALADGLVLADRMLNELAECMRV"
BASE COUNT         1290 a         2589 c         2655 g         1277 t
ORIGIN      
        1 gaaccctccc gtgcagcggt tcgaagccgc gagcgcgcct tcgcccaagg ccgcgaaaac
       61 cccggacaag atcccggata ctccagtcgc caaagcggac gccgccgcgc cgccgcccga
      121 cgccgaagtc gcgaagaagg tcgaccagac gctgctcgag gcgcccgact ccgcggcccc
      181 caccgtcgaa ggcgaaggtt cacccgacgg tgtgaaggag ggaaccgaga cggacccgtt
      241 gaaggcgcga gcgatcagcg cctatcaggc caagctcatc ggctggttca acgcgcgttt
      301 tcgccctccg gagtcgggaa ttccgtgcgc ggagttgaag aagctgagct cgggcgtcac
      361 cgtgagcgtc ggtggcgacc gtcacgttgc cggattttcg gttgggtcgc cgagcggaaa
      421 cgcgattttc gacgagcgcg tacgtacaac attagagcgc gtggtcggcg aggagctccc
      481 tccgcccccg ccgctctacc ccgacatcct cggctcgacc ctttcgtttc gtctttctgg
      541 aagcaaagcc tcatgcgacg acaagccagc gacaccctcc ccgtctcctc cggccacgcc
      601 tgcgcccgaa ggaccggccg cgcccgaggc accagctcct ccgccaagcc cgccgcccgc
      661 gcctccgccc gcgcccgaat cgccggcgcc ggactcgccg ccctgagcct tttcgggctc
      721 gcacagacgg cgctcgccca agagcagggc ggagcggcgc acccggacga gagcgtcctc
      781 ggcgagatgt tggtgacggg gacgacgcaa gagcgcttgc cgcgcatcgc ggtgctcccg
      841 tcgctttcgc cggacatcga agacgtcgtg gtgcgcggcg tcgtgcggcg cgacatcgag
      901 ctcaccggtc tcttcgacgt catccccgac tcgaaggcac cgatgggcct ctatgggttc
      961 gacgatccga tcgatgtcga cgcgtggaag gcgctcggcg ccgaagcgat cgtgaaggtc
     1021 gcggctcgcg ccgacaacag ccccggcaag atccaggtgc tcggcatcgc gtacttcctg
     1081 aacgtcggga aagacccggt gtactcgaag aagatcgtcg tttcgaagaa cgacgcgcgc
     1141 gtcaccgcgc accgcgtgac cgacgcattg ctcggcgcgc tcaccgggcg gcccgggagc
     1201 ttcgccagcg agctcacgtt ttccgcgcgt tgggccaaaa accggcgcat tttcaaggtc
     1261 gacgccgacg gtaacgcgct cacgccgctc acggtggaga accaacacgc catcgccccg
     1321 acgtggggcc ccggccacgc catttactac ttgctgagcg agaactacgg gccctacggg
     1381 ctctaccagt ggaacgcagg gatcaacaag cccgtgagcg tgccgttcaa aacgtcactc
     1441 tacggagttg cgttcgacaa gaccaacacg aagatggccc tcgccgtgag tgagaacgcg
     1501 acgagcggga tctacctcgg caacgtcgac ggcagcggga tgcagcgcat ctcgaacacc
     1561 gacttggcga cgcacccagt gttcagccct tcgggtaagg tcgcgtgggt gggcggcgac
     1621 gcgaagcaag gctcgcagcg tatcttcgtg aacggcaaag cggtgtcgcc ggcggggttc
     1681 accgcggcct cgccggcctt ttgcgacacc gaagacggcg ttcgtctcgt ttactcggtg
     1741 gcggtgggca acgacaagca ggacctcgtc atctcctcgg aggatgggcg cggcatcggg
     1801 cggctcacgc agaaccaggg ctcgaacacg tcgccggcgt gcagcccgga tggtcgcttg
     1861 ctcgccttct tctcgacgcg caacaagcag ccgggcatct acttgatgtc gctcaagcgt
     1921 taccgcaccc agcagatctc gagccagctc ggtgaggcgc tgcgctgggc cgcgttgccc
     1981 ccgacgccgg gcgcaccata gcggcgccct tttcgaaccc gcggatcaga cgaacagcga
     2041 gctcagagcc atcgggatct gggcatcgcc cgagatctgg attttgccgt tcatcatgag
     2101 ctcgaagggg ttggtttcgc cgcgctgcat ggcgaggaag tcgtcgagct gacaccgcac
     2161 gctgcactcg ggcgcctgca cgttgggctg ctgggtgccg ggcgtgagca gcagccggtg
     2221 ctcctcggcg tcggacttga gcacgaaggc gacgctaccg gggacgccgc gaatgatccc
     2281 gactcgctcg gcgtcgagcg tgagcgcgcg caacacgagg agatgtgttt cgctcgactt
     2341 ctgctcggcg gagagcagct cggcgccgcg cacgatgatc ggctcgaaag cgcccgcggt
     2401 caggcgcacc tgagcgatgg tgtcgctcgg aacgccctcc gccacgctga ggcggccggc
     2461 ctcgatgcga atcgcgagcg gcgcgtcttc acccacttgg aacacgagcg ctcccggtga
     2521 gttcttcgcg ccgatgcgcg cggcgagcgg ttcgaaggcc tcgggcaacc agcggccgaa
     2581 aaactcggcg ggttcgttgg ggacggacga catgacggcg gctcagctcc ggatgagcaa
     2641 gctgatagca tgggtctcgc gcgcgccgag cagcccgaac tgaacgccga agccggcctt
     2701 gtcggtccag cgcacgacgg ccggcaattt cagcgagcct ttggttcggg gcaaggtgac
     2761 ctcgatggta agctcggccc cgaaggcaac cggctcattc gactcgagat acatgccgcc
     2821 gatgctgatg tctttggagc gaccctcgaa gctcgtgccg tccttgcgat ggcaagtgac
     2881 gacggttgcg atggctgtcc gaggatgacc gcgcttttcg accacgatct tgttcgtacg
     2941 ctgcgacaca ggtcgtggca acgtccttca ttgattggcc tcgtagtcgg gacgtgcgcg
     3001 aaaggtgccg aggtgcgaca tgggacccgg ctctagctcc gctggttggc gtcgccacct
     3061 cgaaggttgg gagctgggcc tcctggccgt ggtcttcgcg atgctcgcgg tgtggttggc
     3121 tctcccgagg ccagtcgaac cacgtctcgt tccgttgccg gaggtcgatc gccgagtgct
     3181 ggctcgcagc gtcgcgctgg atgtggagcg tgccgtacgt gcgcacgagc gcccgcttgc
     3241 ctacgaggtg cgcgcgttgg gtgagttcgt gcgtcgacac ggcgccgcgg tcgcgcggca
     3301 cgatacgggc gccgccaccg aagcgcgcga cgaagcgcgc aggctttgcc gttcgctcgc
     3361 agcgcgaggc ctcttcgagg agctccaggt gctgcgcgcg acgcagacac ggctcttcct
     3421 cgacgcggtg gagcgctttc gccagggcga aaaggacgac cgagagcttt cggagctcgg
     3481 cgcgaatttc gtggagagag gtcagcgttc gggctggctc gacgcggacg gccgcgtgct
     3541 gctgtcgacc gaagagctgc gcgcgctcta tcggatgcgt tgggccgagc tcacgggggc
     3601 gctcgagatc gggcggctgc ggccgagcct cgacgaattt cgcctctatt atcgcgtctt
     3661 gctcgaacac ccagagggcc tatcgccacc ggagcaggat gagcgccgcc tagcgtacgt
     3721 tacggcgctc gcacgcatcg attcggagtt tccgaccgac ttggcgcgcg gggtgctctt
     3781 ggcgcggttg gggcgggtgc gctccgccta tgacgctttc tccgcgttcg tggtgagcca
     3841 cgagcagggg ccgtgggtgc tgcgagcgcg aaactacgcc ctttgggcgc tcgcccaagc
     3901 tagcccgacg gagtaacact cgccgcgcga tgtccgaagc cgagcctctc gacgacccgt
     3961 tggcggcgct gtcgctcact tccgaccggc gcctcgtgac tttgcgtgcg ctcgcgaccg
     4021 acgtgcttgc ggcacgccgc gttcaccacg tcgtcgacgc gcagggagcg gcgcccgcac
     4081 tggctgcgca cgcgctcgcg ctgagccaat cccggcaagt cttgtacgtg gcggccgacg
     4141 cagacgcggc gcggcgcgct cagtcggatt tggcgggcct gagccaaggt ttgccgctgc
     4201 cgaacgcgct cggggcccct tctggctcgg aggggtcgct gctcctcgcg acgtcggaga
     4261 acactccgta cgccgaggtg cacccggatc ggcgcacggc gatgcaacgc gcggccgggc
     4321 tttttcgcat cgcggcgggg cttccgttcc ggttcgtggt ggtgacggcg gcggcgctcg
     4381 tacggcgcgt ggtgcacccg gcggaggttc gtaaggcgtc tgtgctggtc gaggttcaag
     4441 ctgacttcga cgtcgagcgc acgagccgga tcttggtgaa cgccggttac ctccgagttc
     4501 cggtggtcga agatccgggc acgttcgcgg tgcgcggcgg gctcgtcgac atctggccga
     4561 gcggcgcgga gctcccgctg cgcatcgagc tctacggcga catggtgagc acgctgcggc
     4621 agttcgatcc cgacgaccag cgcacgctcg agtcggtgga gcgagcctgg ttgcctccgg
     4681 cgcgcgaggc ttggatcgac gaagagcaag agcagcgcgc gcggctcgcg ttgcgcgctc
     4741 tgtgcgacga ggtcgacttt ccgtcgagca aggcgcgcgc cttcatcgac gacgtggcga
     4801 gcgggcacgc ctatttcggg ttggagggct atttgccggc gtttcacgag ctcgtgccgc
     4861 tgagcgaata cttttcgcac gacctgccga tcgtcgtcga agacccgccg agcgtggtgc
     4921 gcgccttgcg cgaagagctc gaacgcggac gcgctggtgc ggcggcccgc tccggggctc
     4981 cgcactttgc cttcgagcgc ctctacctcg aagaagacga gctcgcgcgc acgttgagcg
     5041 atcgctccgt tctctgcgtt caccgcgtca gcgtgcaagg cacgagcgag ccggccacga
     5101 tggtcgaggc gctcgaagcc gttccggaag gcgcgccggc gctcagcatt cgcgaccacg
     5161 aagatttggc gcgcgccagt aagaccgcgc gcaccggcaa aggtaaggcg gcgtcgctcg
     5221 acccgctgct caaacggctc gcgatttggc gggaatctgg cctcagcgtg acgatagcgg
     5281 cgcgcacacg cactcaggcc gagcggctcg gcgcgctgct cgggcatcgc ggcatcggcg
     5341 tgcgagctcc agaaagcacg gaggcacccg ggcccggttc ggtgtcgctc gtcgtcgcgc
     5401 cgagcgcgcg cggggcgatc gctcccgccg aaggcttcgt gctgatcacg gaagaggaga
     5461 tcttcggtca gcgcgcccac cgacccgccg cgaagccgcg cgcgaccaag gcgctgctcg
     5521 aagatctgag agcgctcgcg ccaggcgact acgtggtgca cgtcgaccac ggcatcgggc
     5581 gctatctcgg cctcgaaaag aaggacgtcg gcggggtcaa cgtcgatctc atcgtcgtcg
     5641 agtacgcggg cggaaagctc tacctgcccg tctatcggct caaccaaatc gagaagtacg
     5701 ccgcgagcga cacgacgccg aagctcgatc gtttgggagg ccaaaccttc gccaagacga
     5761 aggcgcgcgt cgagcaacgc gtgcgacaaa tggcggacga gctcttgcgg ctctacgccg
     5821 agcgcaacgc gctcaccaag ctgccgctgc cggcgcccga cgacgactac gccgcgttcg
     5881 aggcgagctt cccgtacgac gagacgcgcg accaggcggc ggccatcacc gacgtcatcg
     5941 gcgacctcac cgccgagcgc gtgatggacc ggctcgtgtg cggagacgtc ggcttcggca
     6001 agaccgaggt cgcgctgcga gccgcgtttt tgtgcgcgaa tgcaggccgt caggtggcgt
     6061 tgctgtgccc gactacggtg ctcgcccagc agcactacat gaccttttcg tcgcggctcg
     6121 aggactaccc catcacggtg cgggtgatga gccgcttcca gtcgaagagc gagcacgtcg
     6181 cgaccctcaa gggcttgaag gagggcagcg tcgacatcgt cgtcggcacc caccgcctgc
     6241 tttcgaagga cgtccatttc aagaacttgg gtttgttggt ggtcgacgaa gagcagcgct
     6301 tcggcgtcgc tcacaaggag cgcatcaagc agctgcgcaa cgccatcgac gtcctcacgc
     6361 tgagcgcgac gccgatcccg cggacgctcc agctcgcggt cggcggcttg cgcaacatgt
     6421 ccatcatcac gaccgctccc gtggatcggc gcgcgattcg cacgattacc tcgcagttcg
     6481 acccggagct cgtgcgcacg gccgtcgaac gggagctcga gcgcggcggg caggtttttt
     6541 acgtctataa ccgcgtcgag ggcatctacg aacgcgccga gcggctccag cagctcgtgc
     6601 cccgtgcccg catcgccgtc ggccacggcc agctcagcga agaggcgctc gagcgcacga
     6661 tgctgaactt cgtggcgggc gagtacgaca tcctcgtcgc gaccgcgatc gtcgagagcg
     6721 gcctcgacat cccccgcgcg aatacgatca tcgtcgatcg cgcggacttg ttcggcttgt
     6781 cgcagctcta tcagctgcgc ggtcgcgtcg gtcgtagcag cgagcgcgct tactgctact
     6841 tgctggtgcc gccgcccagc caaatgaccg acgaagcacg ctggcgcctc gaagcgctcg
     6901 agcgacacac ggagctcggt tccggctttc acatcgccgc gctcgatatg gagctgcgcg
     6961 gcgcgggcga cgttttgggc gcagatcaaa gcggcttcgt cgcgagcgtc ggcttcgacc
     7021 tgttctgtca catgctggaa gaagcgacgc acgagctgcg cggcgagacg gtggtgcacg
     7081 aggtcgaccc cgagctcagc gtggacgtgg aggcgctctt gccggaaagc tacgtggccg
     7141 acgtcggcgt gcggctcagc ctgtacaaac gcctcgcgag cgcgaccgag gacggcgagg
     7201 tcagcgacat cgcgaacgag ctcaacgacc gcttcggcga gccgccgatc gaggcgcggc
     7261 gcttcatcga gctcatgcgc ttgaagaccg agctccggcg gttgcgggtg ctcggatgcg
     7321 aagcttcggc gcgctcggtc acgttgcact tgcgcgacga cacgccgctc gacccgcaaa
     7381 aaatcagtgc gctcatcgct cgcaagaagt cgccctaccg gctcagtccg gatggccgct
     7441 tgactcgacg cgcgctcgag aacgaagcgc tcgccgacgg cctagtcctc gccgatcgca
     7501 tgctcaacga gctcgcggag tgcatgcgcg tctagcgggg agcggaaggc acgcacggca
     7561 tcgtcgcaaa acgaaacagt gactccgctg aacggacgtt cgcctgcgcg cccgtgctcg
     7621 cgaaacggag agatacattc cgtctcaccc gagatcttct cctcaaactg aatcagccag
     7681 agcatccgcg agggtgggtt tcagcgtggc cggagtgggg ctctaccgct aagctgtagt
     7741 gcgcgactga gcgtgcgtgg tcacgatgct aggcgtgtcg actctgcaag ctaagtgcgg
     7801 cgacttggtc g
//
LOCUS       DASTKS010000199         7736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_252577, whole genome shotgun sequence.
ACCESSION   DASTKS010000199 DASTKS010000000
VERSION     DASTKS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252577"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFQ35_04165"
     CDS             <1..201
                     /locus_tag="VFQ35_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9929853.1"
                     /translation="YTFYVLTGLHAAHVVGGFVPAGIVFARARRREYSSSRHEGVLLC
                     TQYWHYLGVVWLALFLVLELSS"
     gene            198..416
                     /locus_tag="VFQ35_04170"
     CDS             198..416
                     /locus_tag="VFQ35_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929854.1"
                     /translation="MNTFYWDFFGPRAEPTAVHFRKHLEQFLTENALVGCETGVESQQ
                     PGHFAAFCRTPSEHHDALVAGLRPQRML"
     gene            complement(431..1021)
                     /gene="pdxH"
                     /locus_tag="VFQ35_04175"
     CDS             complement(431..1021)
                     /gene="pdxH"
                     /locus_tag="VFQ35_04175"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9929855.1"
                     /translation="MRDAPNKDPLQAVVDWHREAQTVMARDPDAMTLATATRDGAPSA
                     RIVLFKGVDAGEILFVTNYSSRKGQQIDENPAVALVFYWPELMRQIRVEGIARRSPVD
                     ESEAYFRSRPRESQLGAWASAQSTPIASRAELEQRFAAAAERFGDRDVERPPYWGMYR
                     VRPQRIELWLGGAHRLHDRFQYDRNDESWSVTRLSP"
     gene            1234..2448
                     /locus_tag="VFQ35_04180"
     CDS             1234..2448
                     /locus_tag="VFQ35_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET9929856.1"
                     /translation="MRLGPRLLAFCIVAATSSCVPSAGKVRLSDGSPSNPPTAPAASA
                     PPSETATADDEQPADAADSDSDGEAHSDEPEVVDDESEEEPAAPTVEVKRPHPLDGWS
                     QQKIRAAVHENPASLGSMSIGSPNAGALLNGVRADANELFVPVDPSHAYGTVETVKYL
                     SDAVRKVHAAFADTPALSLGHISAQRGGPLRPHVSHQAGRDVDISFYYVGGARWYTRG
                     TESNLDLPRTWALVRALVTETDVDMILIDHSIQQWLWRYAREHGEDAAWVDSLFQGKG
                     AGRPIVRHAPGHATHLHIRFFNPEAQETARRAHPALVAEGIVPPLVRFVVHHAKHGDT
                     LGKIAKKYAVSVAAIREANGLRSNRIREQRDYRIPLRSETKIALAPELRFPPRRLPPA
                     SASALGPVSARP"
     gene            complement(2452..3600)
                     /locus_tag="VFQ35_04185"
     CDS             complement(2452..3600)
                     /locus_tag="VFQ35_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929857.1"
                     /translation="MGADLLSSQSSPSRLVCRRGRWTLELTRLVAVDNHVFQPRKQRI
                     DGSDLILSHLKRSAEGATLAQGALQLMRAARGDDERSFEAIEGEWVPVQENRRDAEVA
                     FQESAQAALAELRAELLILRASHQRLKERVVSLEARLAGGSVPAPRQPAPAAPGPDPV
                     AAQFSSVGHPNAGKAAASSSPLAPEPAPLFSQAQPPASPTLATGQDPEPARPYGEVVA
                     AAGNQSVAGHQIATGDSNEILNAIKELCGGDPGYVKSEEPLPDSALELAALYASLLVD
                     DDGQALGAVLADIRATAGLGGKLAGLPPTVIEEQAKTGVLNEAVTSAMSEVCNTVSSV
                     LSRVPRNGNLRSTPLESFPADRLRWVNNPSSVVAFEKRRAGVFWIVTR"
     gene            complement(3716..4651)
                     /gene="argF"
                     /locus_tag="VFQ35_04190"
     CDS             complement(3716..4651)
                     /gene="argF"
                     /locus_tag="VFQ35_04190"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9929858.1"
                     /translation="MKDLIRTSDLSPADFKYLLARAQKFKQNPHARRSLLAGESVCLY
                     FAKPSTRTRVSFETAVTRLGGAPISLGPNDVQLGRGESIEDTAQVWSRFAKAIVIRTF
                     KDDDVDRLAKAASIPVINALTDGHHPCQSLADMMTLVEHRGSLKKCRIAYLGDGNNVA
                     ISLMQAAALCGSHIVLGCPKDYSVRPELVEAAQRLAEKTGGSVNVTEDPEAAANGADA
                     LYTDVWLSMGDADAEKEVRQRVLAPYQVSERLMARAKSDAIFLHCLPAHRGEEVSAGV
                     MDGPRSVVWEQAENRLHTAVAVLYALIERKLGRDE"
     gene            4801..5319
                     /locus_tag="VFQ35_04195"
     CDS             4801..5319
                     /locus_tag="VFQ35_04195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9929859.1"
                     /translation="MPNPTATLNTSLGDITVELFTDKMPITAQNFIKLAKSGFYDGLH
                     FHRVIDGFMLQFGCPHSRDPQSPRAGTGNGPDGTIQDEHPADAKLSNEPGTLSMANTG
                     RPNSGSCQFFINTVHNAYLDWFSPGPSKHPVFGKVTAGMDVVKKIEKAETDARDRPIK
                     PVQMIKVTVNDA"
     gene            5746..7470
                     /locus_tag="VFQ35_04200"
     CDS             5746..7470
                     /locus_tag="VFQ35_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9929860.1"
                     /translation="MALPALVACDGQSTIEDIGSGGASGPGNSAGGVAGAASGGASTA
                     GASNGGSASGGMPASGGAPLDCSSAVPPNSPLALLTRVQYDNTVADLLGDSSNPSKVF
                     PPENQVEGFKNNAVANLASPLSVEKYLAAAETLSATARGRINDFAPCASGQDQAECGR
                     TFVRTFGLKAFRRPLTDSEQKVFNDLFETVRARSGYETAFELTLQALLQSPQFLYRAD
                     SLAGPTEQTGAVALGPYELASRLSYFLTGSMPDSTLFTAAAENRLVSDADVEREARRL
                     LTTPRAHGVVREFHHQWFMMDALPSVLRAFPTSFGSDTAWVAKDWLESFDRFIDHVYW
                     ESGSVSELFTSKRVYVNSRLARLYGVTGGGDDFVSAELPDRAGVITQPALMALLAHTD
                     QTAPVQRGVFMLERLMCSAVPPPPPTVKAVPPDPDPNATTRERFDVHTKDPACNGCHR
                     LIDGVGFGFEAYDQLGRYRTTENGLAIDASGEVLISDAELAGSFTGAQELAARLAASS
                     RARDCVATNWYRFALGRLEGEGDRCSLDDVKHRFADSKGDFRELLVAITRSVAFRYRP
                     AIPSDGGP"
     gene            7470..>7736
                     /locus_tag="VFQ35_04205"
     CDS             7470..>7736
                     /locus_tag="VFQ35_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9929861.1"
                     /translation="MSQRVNRRELKARLTRRQLLYGLGGISVGLPLLSSLSARADAMP
                     APKRLVLMYTPNGVINDSWWPTNVTSETVFELNKIHQPLAPYRDK"
BASE COUNT         1360 a         2599 c         2460 g         1317 t
ORIGIN      
        1 tatacgtttt acgtgctcac ggggctgcac gccgcgcacg tggtgggcgg cttcgtcccc
       61 gcgggcatcg tcttcgctcg ggcgcgccgt cgcgaatact cgagctcgag gcatgagggg
      121 gtgcttctct gcacgcaata ctggcactac ctcggcgtgg tctggctcgc gctctttctc
      181 gtactggagc tgtcttcgtg aacacgttct attgggactt cttcgggcct cgcgctgagc
      241 cgacggcggt tcacttcaga aagcacctcg agcagttctt gaccgaaaat gctctggtcg
      301 gctgcgaaac gggagtcgaa agccaacagc cgggccactt tgcggcgttc tgtcggacgc
      361 cgagcgagca ccacgatgcg ctcgtggccg gcctccgtcc tcagcgcatg ctttgagtca
      421 gctcaccgcc tcacggcgag agccgcgtga ctgaccagct ctcatcgttg cgatcgtatt
      481 gaaagcgatc gtgcaggcgg tgcgcccccc cgagccacag ctcgattcgc tgcgggcgaa
      541 ctcggtacat cccccaatag ggaggtcgct ccacgtcgcg gtcaccaaag cgctcggcgg
      601 cagccgcaaa gcgctgctcg agctccgcgc gcgacgcgat cggcgtgctc tgagcggagg
      661 cccacgcacc gagctgactc tcccgcgggc gggaccgaaa gtacgcctcc gattcgtcta
      721 ccggcgagcg ccgggcaatc ccttcgacgc gaatctgccg catgagctct ggccagtaga
      781 agacgagggc gaccgccgga ttctcatcga tttgctgccc tttacggctc gaatagttgg
      841 tcacgaacaa aatctcgccg gcgtcgaccc ccttgaagag gacgatacgc gcggacggag
      901 cgccgtcgcg cgtcgccgtg gcgagcgtca tcgcgtcggg atcgcgcgcc attacggtct
      961 gagcttcgcg gtgccagtcc accaccgcct gcaacggatc tttattcgga gcgtctcgca
     1021 tcgggattcg agcgtgcgcc cacacgccag aacgcgcaac gtgaaaaact ccacaaaagc
     1081 cggacgagac tgcgcaaaat cacgggattc gccgccggct gtgccgcgac atggcggcga
     1141 catcggctat aaccgcgtcg tgggatttca ggtcagccac gtcgcccatg ctgtccaagc
     1201 aagcggcgtc tcgcgccaaa gggtgacggc ggcgtgaggc tcgggcctcg gctcctcgcg
     1261 ttctgcatcg tagccgcgac ctcgagctgc gtcccgagcg cgggcaaagt acggctctcc
     1321 gatggctcgc cgagcaaccc tccgacggcc ccggctgcat cggcgccgcc ttcggaaacg
     1381 gcgacggccg acgacgagca acctgcggac gcggccgaca gcgacagcga cggcgaggcc
     1441 cattctgacg agcccgaggt ggtcgacgac gagagcgaag aagagcccgc ggcgccgacg
     1501 gtcgaagtca aacgccctca cccactcgac ggttggtcgc agcagaagat ccgcgcggcc
     1561 gttcacgaga atcccgcatc gctcggctcg atgtcgatcg gctcgccgaa cgcgggcgcg
     1621 ctcttgaatg gggtgcgcgc ggacgcgaac gagctcttcg ttcccgtcga tccatcccac
     1681 gcttacggca ccgtcgagac ggtgaaatac ctgagcgacg ccgtacgcaa ggtgcacgct
     1741 gccttcgccg atacaccggc gctttccctc ggccacatca gcgctcagcg cggcgggccc
     1801 ctgcggcccc acgtgagcca ccaagccggg cgcgacgtcg acatctcctt ctactacgtg
     1861 ggcggggcac gttggtacac ccggggcacc gaaagcaacc tcgatctgcc gcgcacttgg
     1921 gcgctagttc gagcgctcgt taccgaaacc gacgtcgaca tgatcctcat cgatcattcg
     1981 attcagcagt ggctgtggcg ttacgcgcga gagcatggag aagacgcggc gtgggtggat
     2041 agcctgttcc agggcaaggg ggcaggtcga ccgatcgtgc gtcatgcccc agggcacgcg
     2101 acacacttgc acatccggtt cttcaaccca gaagcacaag agaccgcgag gcgcgcgcac
     2161 cctgccctcg tggccgaagg gatcgttccg ccgctcgtgc gcttcgtcgt gcaccacgcc
     2221 aagcacggcg acaccttggg taagatcgca aagaagtacg cggtgagcgt cgcggcaatt
     2281 cgcgaggcga acggcttgcg ttcgaaccgg atccgagagc agcgcgacta ccgtatcccg
     2341 cttcgctccg aaaccaagat tgcgctcgct cccgagctca ggtttccgcc gcggcggctg
     2401 cctccggcgt cggcgagcgc gctcggtccc gtgagcgctc gcccctgaac gtcaccgcgt
     2461 gacgatccag aagacgccgg cacggcgctt ttcgaaagca acgacgctgc tcgggttatt
     2521 cacccagcgc aggcggtcgg cgggaaacga ctcgagcggt gtgctgcgga ggttgccgtt
     2581 gcgcgggacg cggctcaaca ccgagctgac ggtgttgcac acctcgctca ttgcgctcgt
     2641 gaccgcctcg ttcaggacgc ccgtcttcgc ctgctcctcg atgacggttg gagggagccc
     2701 agcgagcttg ccgccgagcc ccgccgtcgc gcggatgtcg gccagcacgg ctccgagcgc
     2761 ttgtccgtcg tcgtcgacca agagagaagc gtagagcgcg gcgagttcga gcgccgaatc
     2821 tgggagcggc tcttcggatt tcacgtagcc gggatcgcct ccgcagagct ccttgatggc
     2881 attcagaatc tcgttcgaat ctccggtcgc gatttgatgc ccggcgacgc tttggttgcc
     2941 agccgcagcc acgacttcgc cgtaggggcg cgctggctcc ggatcctgac cggtcgccaa
     3001 ggtgggagaa gctggaggtt gcgcttgcga gaacaacggg gccggctccg gcgcgagcgg
     3061 cgacgacgac gcggccgcct ttcccgcgtt cgggtgtccc accgacgaga actgcgccgc
     3121 gaccgggtcg ggccccggcg cagccggcgc tggttggcgc ggtgccggga cgctcccacc
     3181 ggcaaggcgc gcttcgagcg aaaccacacg ctccttcaat ctttggtgcg atgctcgcag
     3241 tatcaggagc tcggcgcgca actcggcgag cgcggcctgc gcgctctcct ggaaggcgac
     3301 ctctgcgtcg cggcggttct cctgaaccgg aacccactcg ccctcgatcg cttcgaacga
     3361 acgctcgtca tcgccgcgcg ccgcgcgcat caattgcaga gctccttgcg caagggtcgc
     3421 accttccgcg ctgcgtttca ggtgggacag aatcaaatcc gacccatcga tccgctgctt
     3481 gcgcggctga aagacgtggt tgtctaccgc taccaggcgc gtcagctcga gcgtccagcg
     3541 cccacgtcgg cacactagcc gcgacggcga agattgactc gagagtaggt cagcgcccac
     3601 gccatccctt aacggcagcg actcgcgtgt ctaaaacaga gattttgcgg caccgatgtg
     3661 ggtcccccag ctcacccaca tcggccgtcg aatccgcccg tcgggcggcc gccgcctact
     3721 cgtcgcgacc gagcttgcgc tcgatgagag cgtagagcac cgcaacggcg gtgtggagcc
     3781 gattctccgc ctgctcccac acgacggagc gcggcccgtc catcactccc gccgatactt
     3841 cttccccgcg atgggctggc aagcaatgca ggaagatggc gtccgacttc gcgcgagcca
     3901 tcagccgctc cgagacctga tacggagcga ggacgcgctg ccgaacctct ttctcggcgt
     3961 cggcgtcgcc catcgagagc cacacgtcgg tgtagagcgc atccgcgccg ttcgccgccg
     4021 cttcggggtc ttcggtaacg ttcacgctgc cgcccgtctt ttctgcgagg cgttgcgccg
     4081 cttcgacgag ctcgggacgc acgctgtaat ctttggggca gccgagcacg atgtgcgagc
     4141 cgcagagcgc cgccgcctgc atcaagctga tggcgacatt gttcccgtca cctaggtacg
     4201 cgatgcggca cttcttcaac gagccgcgat gctccaccag tgtcatcatg tccgccaagc
     4261 tctgacaggg gtgatggccg tccgtcagcg cgttgatgac cggaatgctg gcggctttgg
     4321 ccaggcgatc cacgtcgtcg tccttgaagg tgcgaatcac gatggccttc gcgaagcggc
     4381 tccacacctg agccgtatcc tcgatgctct cgccgcggcc gagttgtacg tcattggggc
     4441 cgagcgagat cggcgcgccg ccgagccgcg tgaccgcggt ttcgaaagag acgcgggtgc
     4501 gggtcgaggg tttggcaaaa tagaggcaga cgctctcacc cgcgagcaac gagcggcgag
     4561 cgtgggggtt ctgcttgaac ttctgtgctc gggcgagcaa atacttgaag tcggcggggc
     4621 tgagatcaga ggtgcgaatc aggtccttca tgaaggggct cccgagcagg gcgggcctgc
     4681 gaaggccgga gaatctccta cggcgctagg aaaacgcaag cccgaggcgc ccggttggac
     4741 aggaattcgc gcaataagac cggattgcca ggcggcgatg gcagaactag cgtcccccgc
     4801 atgccgaatc cgaccgcaac tctcaatacc tcgctcggcg acatcaccgt cgagttgttc
     4861 accgacaaga tgccgatcac ggcacagaac ttcatcaagc tcgcgaagtc tggcttttac
     4921 gacggcttac atttccacag ggtgatcgac ggcttcatgc tgcagttcgg ctgtccccac
     4981 agccgtgacc cgcagagccc gcgcgccggc accggcaacg ggccagacgg aaccatccaa
     5041 gacgagcacc cggccgacgc gaagctctcg aacgagcctg gcaccctgtc gatggcgaat
     5101 acgggccgtc ccaacagcgg cagttgccaa ttcttcatca acacggtcca caacgcttac
     5161 ctcgattggt tcagcccagg gccgtcgaag caccccgtat tcggcaaagt cacggccggc
     5221 atggatgtcg tgaagaagat cgagaaggca gagaccgacg cgcgtgatcg tccgatcaag
     5281 ccagtgcaaa tgatcaaggt gaccgtcaac gacgcgtgat cgcctagcaa cgcggactcg
     5341 ccaagtcatt cgaggcggcg agcagcgtca cccgcgcgac atattgggga tgtctcaccg
     5401 aggcatcccc tcatcccctc gcaatctcga agtgggcgcg agcggcgcgt cgccaaaagg
     5461 cgcaggggca gtcacgtgat cgcgccgcga aaagggcaag ttcgcccgca actcgggcgc
     5521 ttcggcaaag cgaacgcaag cagcgatgag ctcgcgactc agcgctcgaa agttgtcgtt
     5581 ctccggagaa ggcgccattc cgagcggaat cttacctttt ggcaggaagc accacctcac
     5641 cggcgctgat gaatttcaac ggctgaaaat tgtgtcgctt gcgaacgttc gagccgaacg
     5701 taagctcagg gtcgcttgcg cgggttgcgc cgtattctcc ttgccttggc cttaccggcc
     5761 ttggtcgcgt gcgatggtca gtcgaccatc gaggacatcg gttcaggcgg cgcgagcggt
     5821 cccggcaact ccgccggagg tgtggcggga gctgccagtg gtggcgcgag cacggcagga
     5881 gccagcaatg gtggttccgc gagcggcgga atgccagcga gcggaggcgc cccgctcgac
     5941 tgcagcagcg cggttccgcc caattcgcca ctcgctctcc tcacccgagt gcaatacgac
     6001 aacacggtcg ccgatctgct cggtgattcg tcgaatccat cgaaggtatt ccccccggaa
     6061 aaccaggtcg agggcttcaa gaacaacgcc gtcgccaatt tggcaagccc gctctcggtc
     6121 gagaaatacc tcgccgcagc cgaaaccctc tctgcgacgg ctcgggggcg catcaacgac
     6181 ttcgctccct gtgcgagcgg ccaagatcaa gcggagtgcg ggcgcacctt cgtgcgaacg
     6241 ttcgggctca aggcgtttcg ccgtcctctc accgactccg aacaaaaggt cttcaacgat
     6301 cttttcgaaa ccgttcgggc tcgctcggga tatgagactg cgttcgagct gacgctgcag
     6361 gcgctgctcc agtctcctca gttcctctat cgggcggact cgctcgcggg accaaccgag
     6421 cagacgggtg ccgtcgcgct cggcccttac gagctcgcct cgcggctctc gtatttcctc
     6481 accggctcga tgccggactc gactctgttc actgctgcgg ccgagaaccg gctcgtgagc
     6541 gacgccgacg tagagcgtga agcgcggcgc ttgctgacga cgcctcgcgc ccacggcgtg
     6601 gtgcgcgagt tccatcacca gtggttcatg atggacgcgc tgccctcggt gctgcgcgcg
     6661 tttcccacga gcttcgggag cgacacggcc tgggtcgcca aggattggct cgagtcgttc
     6721 gatcgcttca tcgaccacgt gtactgggag agcggctccg tttcggagct cttcacgtcg
     6781 aagcgcgtgt acgtgaactc gcggctcgcg cgcctgtacg gcgtgaccgg cggtggcgac
     6841 gacttcgtga gtgcagagct gccggatcgc gcgggcgtga tcacgcagcc cgcgttgatg
     6901 gcgttgctcg cgcacacgga tcagaccgcg ccggtgcagc gcggcgtgtt catgctcgag
     6961 cgcctcatgt gcagcgcggt ccccccgccg ccgccgaccg tgaaggcggt cccgccggat
     7021 ccagatccca acgcgacgac gcgcgagcgc ttcgacgttc acacgaagga tccggcgtgc
     7081 aacggctgtc accgcctcat cgacggcgtt gggttcggct tcgaggcgta cgatcagctc
     7141 gggcgctacc gcacgaccga gaacggcctt gccatcgacg cgagcggcga agtgctgatc
     7201 tcggacgccg agctcgctgg ctcgttcacc ggggcgcaag agctcgcggc gcggctcgcg
     7261 gccagcagtc gagcgcgcga ttgcgtcgcc accaattggt accgcttcgc cctcgggcgg
     7321 ctcgaaggag agggcgatcg ctgcagcctc gacgacgtga agcaccgctt cgcggattcg
     7381 aagggagact tccgcgaatt gctcgtagcc atcacgcgct cggtcgcctt ccgctaccga
     7441 cccgccattc ctagcgacgg aggtccctga tgtcccagag ggtcaaccgc cgcgagctca
     7501 aggctcgcct cacccgccgc cagcttctgt acggcctcgg aggcatcagc gttggcctgc
     7561 cgctgctgtc gtcgctctcg gcgcgcgccg acgccatgcc cgcgccgaag cggctcgtct
     7621 tgatgtacac gccgaacggc gtcatcaacg attcctggtg gcccaccaac gtcacgagcg
     7681 agaccgtctt cgagctcaac aagattcatc agccgctcgc gccctaccgc gacaag
//
LOCUS       DASTKS010000200         9842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1145697, whole genome shotgun sequence.
ACCESSION   DASTKS010000200 DASTKS010000000
VERSION     DASTKS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145697"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1241
                     /locus_tag="VFQ35_04210"
     CDS             363..1241
                     /locus_tag="VFQ35_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9929862.1"
                     /translation="MNLERLELFLKIVDTGSVSAASREAHLTQPAVSRSLKLLEEDIG
                     SDLFDRQGRGLLLTAAGRALIPWARQLVREAERARLQVQRVAKSACYDLRLGAVDSIG
                     THVLPNCLAEVQERHPDIKLKLWMGRTQTLLERVERGTLDLALVAYSAPPSEGAQRLG
                     SYGLKYYGSKERYPELATLTSELELRRFPIVELEALPGQPSLIHDDAEAFAVTQNLSA
                     VKALVLAGLGVGQLLPFMLSAAENDALVCAERVQHDPDCGIYAVRSPRALSDVEAAIQ
                     QDLVTTLKHTFAHRGM"
     gene            1491..2624
                     /locus_tag="VFQ35_04215"
     CDS             1491..2624
                     /locus_tag="VFQ35_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929863.1"
                     /translation="MIGEGKGSTATESSLPTCFEPSEGGGVRVAPEAALPELAKSAAK
                     APDSIVNVIAKLAALDEQRGCGPDSDYFWPRQDCAKAKYIELELEAGSRERTRAAAKA
                     SWFGGGLEVGREYRWSLQVCAGANPGVRPVAIVLGYEATGRAAGGKPEECKTDAWRAW
                     PERDLRQLSVNEVLPGGFRTKGFVATCFKPQPCPPHALCKPQAAPNALFAADASLKGP
                     FLRVDGACDGRYRVGAGYELAVVLHSRDHLGFVNSGYACAAQALSADATDAPSAKAPE
                     TKVADEAAPKNPEQAWVGLSAAEVSRAATGERGGWGPRDVSYSGGPPRVTRRGDMWTL
                     EWVPGGCSDFSSMLTLHFVQGRVRRVEFEPRHRHTGMECALNE"
     gene            3127..4401
                     /locus_tag="VFQ35_04220"
     CDS             3127..4401
                     /locus_tag="VFQ35_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9929864.1"
                     /translation="MPIETSFVACNTGWLHRPKAEACPSFLPRDQGSPSTPKPEDECA
                     RDEDCTARPHGYCGAAQYSQLSPASYRACHYGCTQDSDCGEGLCVCSYPVGECTSHRD
                     CRVDADCASGLCVGVRTAASACGESFYQFACHSPNDACPATASCGSGGACLWAADGRY
                     CENIGICGRPFLVDGVARLATAAVGRGWASDDSVALAALTDIERQRLAAHYERNALME
                     HASVAAFARFALELLAFGAPAELLRETQRAMADEIRHAELCFGLASEYAGHAVAPGPL
                     SVNGAVTTPELETALLTAFLEACIGETQAAAEATAALLGATEPAVVAALGQIAEDERR
                     HAELGWRFVQWALTRLSAPRRVALSQRLVQEVRIALERASEPLSPVGSAAEMAHGLLA
                     PATLQEARYAALAEVCLPCAEALAYPALQVAC"
     gene            complement(4448..5404)
                     /gene="nadA"
                     /locus_tag="VFQ35_04225"
     CDS             complement(4448..5404)
                     /gene="nadA"
                     /locus_tag="VFQ35_04225"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET9929865.1"
                     /translation="MATLASPSPDLASLDLDEEIRRLKRERNAVFLAHYYQESEIQDL
                     ADFIGDSLQLAQAAQRTQADVILFAGVHFMAETAKILNPTRTVLVPDMKAGCSLADGC
                     PVDRFKAWKARHPGSVVISYINCSAAVKAESDYICTSSNAEKIVRAIPADKKILFAPD
                     KNLGRYLVKKTGRDMVLWQGSCIVHETFSLRKLEALMVEHPEAKIIAHPECEEALLER
                     AHFIGSTAALLKFVQADPAREFIVVTEPGILHQMHKAAPDKVLIPAPPEANCACNECP
                     FMRLNTPEKVYLALRDLSPELTMPEDLRERAKLPIERMLALS"
     gene            complement(5511..6506)
                     /gene="thyX"
                     /locus_tag="VFQ35_04230"
     CDS             complement(5511..6506)
                     /gene="thyX"
                     /locus_tag="VFQ35_04230"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9929866.1"
                     /translation="MNETKRPVSAGAEELLGRYFPVLDHGFVALVDYMGTDECIERAA
                     RVSYGYGTRKTSVTRGLLRYLRRHLHTTPSEMVELKFHCCMPMFVARQWIRHRTANVN
                     EYSGRYSLMPMLFYTPSAEQLQTQSATNNQGRSGDAVDVELHRDAVRRWNEIRKHSRD
                     TYEWMVGQQMARELARIDLPLSTYTQWYWKVDLHNLLHFLRLRVDPHAQWEIQEFGRV
                     MAGMLKRVAPLSYEAWIDYDVCGARLSRMELDALRRLVRVEGSSLTTAGGERIDEAGL
                     EGLGLARREVRELLNKLSQSSVPDFELDLSRSEPPETFAARFASAVPKVDTTPKE"
     gene            6684..9548
                     /locus_tag="VFQ35_04235"
     CDS             6684..9548
                     /locus_tag="VFQ35_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929867.1"
                     /translation="MHKFARLGSRVALGAIVTFACSAFAEGRACAYARLVSPIEGPDV
                     NSLGIEADGTRRTVSHGLRALVHSDGSVEMAEEVFPLARGVGLVELPRRFGGGFLFAM
                     SGSGRASLWFAKTFTGKLRPFAALDFEIERIVPGFDRLYVQARRSSEWVALEADTGAG
                     TDRGSLPAAANYGALAFADAWFGAVEVPIQGVVVSFDAGGTWHSLGGKMRLIGEERGE
                     IMLMAPDGAKKLSSDGTLRSVEGAEAKSSAVRRNTVEGPAGANPLANAMLRGIADSST
                     TALVLYRGALSRVRIADGRVLDSRPRVVPPSAVCSGLPLGKGLGFACQEPRGKMHLLA
                     VKTGLSVEVVQTFESPRDVISSGNGALVIRGGCGPTPSEASRTDVHCVRPPSGASFEV
                     AHGERAVRVIGLRDGGAALIVPPERDAPGTLERVSPSGKRGSPVTLTPDDHDDGVKGL
                     ITKGFWLDAWIENEKGELSGWVAGQSSFAGVRVRSDGHLQVGVMRRRIERALVSGDRV
                     LLIAAAGIAEQSIDGGKTYTDIDLPPELMLEPQKVQGFGLPLEQGCSPLGCAFAGWAR
                     VGWDGPEGGKPLAVAATPKPASFPQPGGGRWALRCAPTGDVSAPAIAVAPASGTGEGQ
                     VAPWLPLWEQAPPARPRDSLAFDVGSEGQLRAYVWTPRGADFGKAGRFAASILDPYRV
                     QGGVWRTLVGPSPWSDPTQVGEIFGYEGSVSSSWRLNLDASGHAGVLSVSARGTTELF
                     AVEENRGIVPLLNASRAGVGTIASAVEMETGFYVAALEEPRNLRVFALEGGDARLLGQ
                     YSDLSQGRGLPVLVRSTRRDSLAIWTRGAGWFIFPIDPRTGAASSAIEVTPRSLSRFP
                     RVCEPDEDGYLLEGPVGVEPYAEFVAGAEDVVASGFSGRFVVSERGVCVVSLAARADD
                     SIERKVSIKAKASAGDGFRIPLVVSDRSERGRRWGFRCGK"
BASE COUNT         1552 a         3135 c         3395 g         1760 t
ORIGIN      
        1 cgaccaagta ggagccggcc gaagtctgca cagtggtgat ggtggtcacc ttgaccactt
       61 cgagcggtat gctcggcggg tcttcggcct tcgtgcccgt gctcccaccg ctgctcccgc
      121 cgctgcacgc ggaggtcacg ataaaaagcc cgatcgcggc tagccgaagg gcacccggct
      181 tcgataacgg cctccgcgac ccgcagcgtg tgcttggcat gggaagtgat agtctcatcg
      241 ttcgtcacac gtcgccagcg agtggaccat cgtcaccttg ctcgtcgaaa tttcgccaat
      301 agtaacaccg ccctcccaag cgacgtatgc aaccttcgca tggacatcca tagacgtttt
      361 gcatgaacct cgaacgcctc gagcttttct tgaaaatcgt cgacaccggc tcggttagcg
      421 cggcgagtcg agaagcccac ctgacccaac cggcggtgag tcgcagcctc aaactcttgg
      481 aggaggacat cggcagcgat cttttcgatc gccaagggcg tgggctcttg ctcactgcag
      541 ccgggcgggc gctgatcccc tgggcgcggc agctcgtcag agaggcagag cgcgcacgcc
      601 tgcaggtgca gcgtgtagcg aagagcgcgt gttacgacct acggctcggg gccgtcgaca
      661 gcatcggtac gcatgttctg ccgaactgtc tcgcggaggt tcaggagcgg cacccggaca
      721 tcaagctcaa gctctggatg ggccgaacgc agacgctcct cgagcgtgtc gagcgcggca
      781 ccctcgacct tgcgctcgtt gcgtattcgg cgccaccgtc cgagggcgcc cagcggctcg
      841 gcagctacgg cctgaagtac tacggctcga aggagcgcta cccggagctc gcgacgctga
      901 cctccgagct cgagctccgg cgctttccca tcgtcgagct cgaagcgttg ccgggtcagc
      961 cttcgctcat ccacgatgac gcggaggcct tcgccgtgac ccagaacttg agcgccgtga
     1021 aggcgttggt gttggctggc ctgggcgtcg gtcaattgct gccgttcatg ctcagcgcgg
     1081 ccgaaaatga cgcgctcgtg tgcgctgaac gagtgcagca cgatcccgat tgcggcattt
     1141 acgcggtgcg ctcgccgcgc gcgctcagcg acgtcgaagc cgcgatccag caagatctcg
     1201 tcaccaccct gaagcacacc tttgctcatc gcgggatgta aacgtccggg ctttgcggag
     1261 cgcgagtgcc ttgcttggat ggagtgaagc gtcgcgctaa agtgcgaggg atgagtgggg
     1321 aaagcgtatc gtgtcgacat ccggggcgtc cggggagatc tgtattcgcc gtggcaatgg
     1381 tggttgcttg cgctcggtcg tcgccgccga ttgaaacgcc ggctgtaccg agcgcgacag
     1441 ccaacgttgc gactcgaagc gttagctcgt ctgcgcagcc tgcggcggta gtgatcggag
     1501 aggggaaggg ttcgacggcc acggagtcgt cgttacccac ctgtttcgaa ccgagtgaag
     1561 gcggcggggt tcgcgtcgct ccggaagccg ctctcccgga gctcgcgaag agcgcggcga
     1621 aggcgcccga ctcgatcgtc aacgtgatcg cgaagctcgc ggcgctcgac gagcaacgcg
     1681 gctgcgggcc ggacagcgac tacttttggc cgcgtcagga ttgcgccaag gcgaagtaca
     1741 tcgagctcga gctcgaagct gggtctcgcg agcggacccg cgccgcggcg aaagcgtctt
     1801 ggttcggggg cgggctcgag gttggacgcg agtatcgttg gtcactgcag gtttgcgctg
     1861 gtgcgaaccc gggtgtgcgc ccggtagcaa tcgtgctcgg ctacgaggca accggcaggg
     1921 ccgctggtgg caagcccgaa gagtgcaaga ccgacgcttg gcgcgcgtgg cccgagcgcg
     1981 accttcgaca gctgagcgtc aatgaagtcc tgccgggcgg ctttcgcacg aaaggcttcg
     2041 tcgccacctg cttcaagccc cagccgtgtc cgccccacgc gctttgcaaa ccgcaagccg
     2101 caccgaacgc gctcttcgcc gctgatgctt cgctgaaggg gccatttttg cgcgtcgacg
     2161 gagcttgcga tggtcgctac cgtgtgggcg ccggctacga actcgcggtc gtgctgcact
     2221 cgagggatca cctggggttc gtgaactccg gatacgcctg tgcggcccag gcgctttcgg
     2281 ccgatgcaac cgatgcaccg agtgcgaagg cgccggagac gaaagttgcc gatgaggctg
     2341 cgccaaagaa tccggagcaa gcgtgggttg gtctcagcgc cgcagaggtc tcgcgcgccg
     2401 cgacgggcga gcgcggcggc tggggtccca gggacgtttc gtactccggc ggaccgcctc
     2461 gcgtcacgcg acggggcgac atgtggacgc tcgaatgggt cccggggggg tgcagcgact
     2521 tctcctcgat gctgactctt cacttcgtcc aaggtcgagt gcggcgagtc gagttcgagc
     2581 cacgccatcg ccataccggg atggaatgcg cgctgaacga gtaacgtctt cgaccctctc
     2641 gagctggctc aacttggcac tgccgattgc gattctcgaa gattcgtagt cggcatgaga
     2701 ggtgcatcgc tagctgccat gaggacgcgt tgggtcgagt ggagactcgg gctactggca
     2761 acgttgggat tgggagcagt ggctggctgc ggcggttcga ccgagagcca ttcgtcgagt
     2821 tatggcggtg gcgctggtgc agggggcact gccggtgctg caaagggcgg ctcttcagcc
     2881 gcgggaagcg gcggaatctc gaccgcggga agtggcggct cgccgaacgg cggctcgccg
     2941 aacggcggag cgtcaagcgc aggaagcggc ggagcgtcga gcgcaggaag cggcggagcg
     3001 tcgataggcg gaggtggcac cggacagggc ggagcgcttt ccggcggagc accaccgaca
     3061 gcgggtgctc caacttgtgc gggtgcagct ccgatgacgg tgcgggctta cggcgcggcg
     3121 ggatccatgc cgatcgagac cagcttcgtc gcatgcaata cgggctggct tcaccggccc
     3181 aaagccgaag cctgtccgtc gttcttgccg cgcgaccaag gctcgccttc gacccccaag
     3241 ccagaagacg agtgcgcgcg ggacgaggac tgtacggcgc gcccgcacgg ctattgcggc
     3301 gcggcgcaat acagccagct cagccctgct tcctaccgcg cttgtcatta tgggtgcacc
     3361 caagattccg actgcggcga ggggctctgc gtttgcagct accccgtcgg cgagtgcacg
     3421 agccaccgcg actgtcgcgt cgacgctgat tgcgcgagcg gtctctgcgt cggagtcaga
     3481 accgccgcga gtgcgtgtgg tgaatcgttc taccaattcg cgtgtcactc gccgaatgac
     3541 gcttgccccg ccaccgcgag ctgtggaagc ggcggagcct gcctttgggc agcagacggt
     3601 cgatattgcg aaaacattgg catttgcggt aggccgtttc tggtggacgg cgtcgcacgc
     3661 ctggctacgg ccgcggtcgg gcggggctgg gcgagcgatg attcggtagc gctcgcggca
     3721 ctgaccgaca tcgagcggca gcgcctcgcc gctcattacg agcgcaatgc gttgatggag
     3781 cacgcctcgg tggctgcctt cgcgcgcttc gcgttggagc ttttggcatt cggagcaccg
     3841 gcggagttgc tgcgcgaaac gcagcgcgcc atggcggacg agatccggca cgccgagctc
     3901 tgctttggcc tggcgagcga gtacgctgga cacgccgtcg cgccgggacc tctctcggtc
     3961 aacggagccg tgaccactcc cgagctcgag acagctctcc tcacggcctt cctcgaggcc
     4021 tgcatcggtg agacccaagc ggctgccgaa gcgacggctg cgctgctcgg tgcgaccgag
     4081 cccgccgtag tcgccgcgct aggccaaatc gccgaggacg agcgccgtca cgcggagctc
     4141 ggctggcgtt tcgtgcaatg ggcgctcacc cggctcagcg cgccgcgtcg cgtcgcgctc
     4201 tcgcagcgct tggtgcagga agtgcgaatc gctttggagc gagcatccga gccgctctct
     4261 cccgtgggat cggcggcgga gatggctcac ggcctcctgg cgccagcgac tctgcaagag
     4321 gcacgttacg ccgcgctcgc ggaggtctgc ttaccctgcg cggaggcgct cgcgtacccc
     4381 gcgctgcaag tcgcttgctg agccgcggtt tcgcggcggc tcgaaaagcg acgaccgctc
     4441 gggtgcctca gctgagcgcg agcattcgct cgatcgggag cttcgcgcgt tcgcgcagat
     4501 cttccggcat ggtgagctcg ggggacagat cgcgcagtgc gaggtacacc ttctccggtg
     4561 tattcaggcg catgaagggg cactcgttgc acgcgcagtt ggcctcgggc ggggccggaa
     4621 tcaggacctt gtccggagcc gccttgtgca tctggtgcaa gatgcccggc tcggtcacga
     4681 cgatgaactc gcgcgccgga tccgcctgaa cgaacttcaa gagagccgcg gtcgagccga
     4741 tgaagtgcgc tcgctcgagc agcgcttctt cgcactcggg gtgcgcgatg atctttgcct
     4801 ctgggtgctc gaccatgagc gcctctagct ttcggaggct gaacgtctcg tggacgatgc
     4861 agctgccttg ccagagcacc atgtcacgcc cggttttctt gacgaggtag cggcccaggt
     4921 ttttgtccgg cgcgaacaag atcttcttgt cggcggggat cgcgcgaacg atcttttcgg
     4981 cgttgctcga ggtgcagatg tagtcgctct cggccttcac cgcggcgctg cagttaatgt
     5041 agctgatgac gaccgagccg gggtgccgcg ccttccaggc cttgaagcga tccaccgggc
     5101 agccgtcggc gagcgagcag cccgctttca tgtccggcac caacacggtg cgcgtcgggt
     5161 tcaagatctt cgccgtctcg gccatgaagt gcacgcccgc gaacaagatg acgtcggcct
     5221 gggtgcgctg cgccgcctgc gcgagctgca gcgagtcacc gatgaagtcg gcgaggtcct
     5281 ggatctcgct ctcttggtag tagtgcgcca ggaagacggc gttccgctcg cgcttcaggc
     5341 gccgaatctc ttcgtcgaga tccaatgagg cgagatcggg cgagggacta gcgagcgtgg
     5401 ccatgtcgcc tatttagcgc tgcaggtgcg cgttcgccat cccgcgatcg gcgaggaaag
     5461 cgaggagcct tcgggcggcg gcgagcgaag cgcacctaaa tggctcgcgc ctactccttc
     5521 ggagtcgtgt cgactttggg caccgccgaa gcgaagcgcg ccgcaaaggt ctcgggcggc
     5581 tcggagcggc tcaggtcgag ctcgaagtcg ggtacgctgc tctgcgagag tttgttcaag
     5641 agctctcgta cttcgcgtcg cgctagacca agcccttcga ggcccgcctc gtcgatgcgc
     5701 tctccaccgg ccgtcgtgag cgatgagcct tcgacgcgaa cgaggcgccg cagcgcgtcg
     5761 agctccatgc ggctcagacg tgcgccgcag acgtcgtagt cgatccaagc ctcgtagctg
     5821 agcggcgcga cccgcttcag catgcccgcc atcacccggc cgaactcctg aatctcccac
     5881 tgggcgtggg gatcgacccg tagccgcaaa aagtgcagca agttgtggag atcgaccttc
     5941 caataccatt gcgtatacgt cgaaagcggg aggtcgatac gcgcgagctc gcgcgccatt
     6001 tgttgcccga ccatccactc gtacgtgtcg cggctgtgct tgcggatttc gttccagcgt
     6061 cgcacggcgt cgcgatgcag ctcgacgtcg accgcgtcgc cgctgcggcc ctgattgttg
     6121 gtggcgcttt gggtctgcag ctgctcggcg ctcggcgtgt agaagagcat cggcatgagc
     6181 gagtaacgtc ccgagtactc gttgacgttg gcggtgcggt gacgaatcca ctgacgggcg
     6241 acgaacatcg gcatgcagca gtggaacttg agctccacca tctcgctcgg tgtcgtgtgc
     6301 aggtgacggc gcaagtagcg taacaagccg cgagtcacgc tggtcttgcg ggtgccgtag
     6361 ccgtagctga cgcgcgccgc gcgctcgatg cactcgtccg tccccatgta gtccacgagc
     6421 gccacgaagc cgtgatccaa gacggggaag tagcgtccca gaagttcttc ggcgccggcg
     6481 gagacgggtc gtttggtttc gttcatgcgg gggcctcggt atcaccgcgt cgtggcggtt
     6541 cggcaagctc tgccacgcct cggccgaacg gccgcgcttc cttcgcgccg caatcgttgg
     6601 ggaatcgttc cgaggcgacg tgcgctatgt cacgtcgcgg gtcctgtcca cgtcgccaaa
     6661 aaccgctacg ctccccaggc tcgatgcaca agttcgcgag gctcggcagc agagtcgcgc
     6721 tcggcgcgat cgtgacgttc gcgtgctcgg cgttcgccga gggccgcgcg tgcgcatacg
     6781 cgcgcctcgt ttcgccgatc gaaggccccg acgtcaactc gctcggcatc gaagcggacg
     6841 ggacgcgccg taccgtatcg cacggccttc gcgccttggt gcactcggac ggctcggtcg
     6901 aaatggccga agaggtgttc ccgctcgcgc gcggtgtcgg gctcgtcgag ctgccacgcc
     6961 gcttcggcgg aggcttcttg ttcgcgatgt cgggctccgg ccgcgcgtcg ctttggttcg
     7021 ccaagacctt caccggcaag ctcaggccgt tcgccgcgct cgacttcgaa atcgagcgca
     7081 tcgtgcccgg tttcgatcgc ttgtacgttc aagctcgtcg cagctcggag tgggttgcgc
     7141 tcgaggccga cacgggcgcc ggcacggatc gcggcagcct tccggcggct gcgaactacg
     7201 gcgcgctcgc cttcgcggac gcgtggttcg gcgccgtcga agtgccaatc caaggagtgg
     7261 tcgtcagctt cgacgctggg ggcacctggc actccctcgg cgggaagatg cgcctcatcg
     7321 gcgaggagcg cggcgagata atgttgatgg cgcccgacgg agcaaagaag ctgtcgtcgg
     7381 acggcaccct gcgctcggtc gaaggcgcgg aggccaagag ctccgccgtg cggcgcaaca
     7441 cggtcgaagg gcccgcgggc gcgaacccgc tcgccaacgc catgttgcgc ggtatcgccg
     7501 actcttccac gaccgcgctc gtgctctacc gaggcgcgct ctctcgtgtg cgcatcgccg
     7561 acggacgcgt gctcgattcg cgtccccgcg tggttccacc ttcggcggtg tgctccggat
     7621 tgccgctcgg caaaggcctc ggcttcgcct gccaagagcc gcgcggcaaa atgcacctct
     7681 tggcggtaaa aacagggctc tctgtagaag tcgtgcagac tttcgagagc ccgcgcgacg
     7741 tcatctcgag cggaaacggc gcgctcgtca ttcgaggcgg ctgcggcccg actccgagcg
     7801 aagcctcgcg gaccgacgtg cactgcgtgc gcccaccgtc cggcgcttcg ttcgaagtcg
     7861 ctcacggtga gcgcgcggtg cgcgtcatcg gcctgcgtga cggcggtgcg gcactcatcg
     7921 tcccgcccga gcgcgacgcc ccggggacgc tcgagcgagt gtctccgagc ggtaagcgcg
     7981 gcagcccagt gacactcaca ccggacgatc acgacgacgg agtgaagggg ctgatcacga
     8041 agggcttctg gctcgacgcc tggatcgaga acgaaaaggg tgagctctcg ggttgggtcg
     8101 cagggcagag ctcgttcgcg ggagtgcgag tgcgcagcga tggtcacctt caagtcggcg
     8161 tcatgcgtcg gcgcatcgag cgtgcgctcg tttccgggga tcgcgtgctc ctcatcgccg
     8221 cggctggcat cgccgagcaa agcatcgacg gcggcaagac ctacaccgac atcgatttgc
     8281 cgccggagct catgctcgag ccgcagaagg ttcaagggtt tggcctgccg ctcgagcagg
     8341 gctgttcgcc gctcggctgc gctttcgcgg ggtgggctcg cgtcgggtgg gatgggccag
     8401 agggcggcaa gccgctcgcc gtcgcggcaa cgcccaaacc tgcatctttt ccgcagcctg
     8461 gcggaggacg atgggcgctc cggtgcgcgc cgaccggcga cgtatcggcg cccgctatcg
     8521 cggtggcgcc cgcctccggc actggagagg gacaggtcgc gccttggttg ccgctttggg
     8581 agcaagcgcc gccggctcga ccgcgtgact cgctcgcgtt cgacgtgggc tccgaaggcc
     8641 aactgcgcgc gtacgtatgg acaccgcgcg gtgccgactt cggtaaagcg ggtcgatttg
     8701 cggcatccat tctcgacccc tatcgagtgc agggcggcgt ctggcgaacg ctggtaggac
     8761 cgagcccgtg gtccgacccg acgcaagtcg gggagatctt cggttacgag ggctccgtat
     8821 cgtcgtcgtg gcgcttgaac ctcgacgctt cgggacacgc aggcgtcctc agcgtgagcg
     8881 cgcgtgggac gaccgagctc ttcgccgtcg aagagaaccg cggcatcgtt ccgctgctga
     8941 acgcctcgcg agcgggcgtg ggaaccatcg cctccgcggt ggagatggag accgggttct
     9001 acgttgcggc gctcgaggag ccgcggaacc ttcgcgtgtt cgcgctcgag ggcggcgacg
     9061 ctcgcttgct cgggcagtac tcggatttgt cgcaagggcg cgggctcccg gtcttggttc
     9121 ggagcacgcg gcgcgactca ctcgcgattt ggacgcgcgg cgcgggctgg ttcatctttc
     9181 cgatcgaccc gcgcaccggc gcggcttcga gtgccatcga ggtcacgcct cggtcgctgt
     9241 ctcgcttccc acgggtttgc gagcccgacg aagatggcta cttgctcgag ggcccggtgg
     9301 gcgtcgaacc gtacgccgag ttcgtcgcgg gcgctgaaga cgtggtcgcg agcgggttca
     9361 gtgggcgctt cgtggtgagc gaacgtggcg tctgtgtcgt gtcgctcgcg gcgcgcgcgg
     9421 acgactccat cgagcgcaaa gtttcgatca aggcgaaggc atctgccggg gacggcttcc
     9481 gcattcccct ggtcgtgagt gatcgctccg agcgtgggcg gcgctggggc tttcgttgtg
     9541 gaaagtgagc ttctcgcccg agagacgtac acggctcttc tggctcgcat atcaccccct
     9601 ggagataagg gcagaaccgc caagagcgcg aatccgcgct agccctcggc gcataaagtg
     9661 aggttgcttt tcggaggtcc ggctgctacc acagtcgggt ccgcgtggag cgaggctgtc
     9721 ccccccccgg ccccttcacg cggacgctta cgtctcgccc gtccgagtcg ctgcggctcg
     9781 ctcgcgacag acttatccat tcgcgggccc tgagcgcaat gccgttgacg cgacagactt
     9841 at
//
LOCUS       DASTKS010000201         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_478024, whole genome shotgun sequence.
ACCESSION   DASTKS010000201 DASTKS010000000
VERSION     DASTKS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478024"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..621
                     /locus_tag="VFQ35_04240"
     CDS             223..621
                     /locus_tag="VFQ35_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929868.1"
                     /translation="MLNRRAFLLALAACAACRGESGPKDPIWGKQPCAHCNMLVSDPL
                     YAAQAESARGDRLFFDDVGCMASYLTEHRGTTKAWVRDAKGNWLEAERARFRLGARTP
                     MDFGVVVDAAGSLDFAAVKERVAAKARGES"
     gene            621..1460
                     /locus_tag="VFQ35_04245"
     CDS             621..1460
                     /locus_tag="VFQ35_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9929869.1"
                     /translation="MFSGRRCGALLRLELADAVRSRWLSFVLVSYTLVFGAFGWLGLR
                     ESSVLGFTGLSRVVLNAANAVVVLLPLVALVATSQAVVRARSSGLFELVLSQPCRVSE
                     WFAAVVAARLLVLAGPLLLVLSALAVSGQLTNEPETLGVVARAALVSATLIACFTGLG
                     LCVSAYARSSERAVVAALLVWLWVGALHDLALIGVLLGLRVEAHAVFALAALNPVEAA
                     RLALLSSVDPELSVLGPVGFWLSNELGAKRTLLVGTLWPLVLTAGSLLVALRRLQRAD
                     LVG"
     gene            1491..1919
                     /locus_tag="VFQ35_04250"
     CDS             1491..1919
                     /locus_tag="VFQ35_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9929870.1"
                     /translation="MSVRNRLIVPCFSLLVVFGCAKKEAPSQQAEPAAAAPAPAAAAP
                     TAEDPAREARNLFKTRCVVCHGEEGKGDGPGAAALNPKPRDYTDAAWQASVTDEQIHK
                     TIVFGGAAVGKSPNMPGNPDLESKSAVVDELVKRVRSFKK"
     gene            1933..2439
                     /locus_tag="VFQ35_04255"
     CDS             1933..2439
                     /locus_tag="VFQ35_04255"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET9929871.1"
                     /translation="MRVLYLASVWLHILAATVWIGGMAFLVLVVVPWLRQRGSADAGK
                     LLRETGARFRTVGWSCFGIVVITGAFNLWCRGVRLSDFARAEWLGSPFGHTVLWKLSV
                     FALVLVLSAIHDFRTGPRATAALERDPTSSEAQRLRRIASLFGRVNALLGLLLVAIGV
                     ALVRGLPW"
     gene            2433..>3141
                     /locus_tag="VFQ35_04260"
     CDS             2433..>3141
                     /locus_tag="VFQ35_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET9929872.1"
                     /translation="MVRPATSAEPLIAPALLRRSVRTETAPGGNAWLRKGFRPFFALA
                     AAYGALIIPLWLLILNGLVRVPGLRDPLLWHAHEMLFGYTTAVVAGFLLTAVGRWSGK
                     ETAVGGPLLALAVLWLLGRVAMLSPFVPLGLAALLDAAFLPALAFAVGRPLILARNRR
                     NYGILGVILLLALANVGTHLDAFGVGGWARQSYLFALDLMVLLMVVVGARVIPMFTRN
                     ALGDSSVRSDVLWDRAAL"
BASE COUNT          408 a          994 c         1101 g          638 t
ORIGIN      
        1 gccgaccgct aacctcgacc tgcgcgctcg cgagcggttc ttccaattgg tggatgcgcg
       61 cgaacgtgaa cggaccttga tcctgtgttc gcatcgggcc gacgacgtgg ctcgcttggt
      121 gcatcgggtc atcgagctcg acgagggccg cgtgacgtcc gacaaatata tcgaagatag
      181 atataatgag ccggtgccag agtggcgcga ggagaggctc ccatgttgaa tcgacgcgct
      241 tttctgctgg ctttggccgc ctgtgcggct tgtcgcggcg agtcggggcc gaaggatccg
      301 atctggggta agcaaccatg cgcccactgc aacatgctcg tgagcgaccc tctttacgcc
      361 gcgcaggccg aaagcgcgcg cggcgaccgg ctgttcttcg acgacgtggg ctgcatggcg
      421 agctacctca cggagcaccg cggcaccacg aaggcgtggg tgcgcgacgc gaagggcaac
      481 tggctcgagg ccgagcgcgc ccgcttccgc cttggcgcgc gcacgccgat ggatttcggt
      541 gtcgtcgtcg atgccgcggg ctcgctcgac ttcgcggccg tgaaggagcg ggtcgcagcc
      601 aaggcccggg gggagtcgtg atgttctcgg gaaggcgttg cggggcgctc ttgcggctag
      661 agctagcgga cgcggtgcgc tcgcgctggc tttcgttcgt gctggtttcg tacacgctgg
      721 tgttcggcgc gttcggctgg ctcggcctac gcgagtcatc ggtgctcggc ttcacgggct
      781 tgtcgcgcgt ggtgctgaac gcggcgaacg cggtcgtcgt cttgttgccg ctcgttgcgc
      841 tggtcgccac gtcgcaggcg gtggtgcggg cccggagcag cggcctgttc gagctcgtcc
      901 tgtcgcagcc gtgtcgagtc agcgagtggt tcgcggccgt cgttgcggcg cgcctgttgg
      961 tgctggcggg gccgcttttg ttggttcttt cggccctcgc cgtctcgggt cagctgacca
     1021 acgagcccga gacgctcggc gtcgtcgcgc gggctgcgct cgtcagcgcg acgttgatcg
     1081 cgtgcttcac cggcctcggc ttgtgcgtct cggcttacgc gcgtagcagt gaacgcgccg
     1141 tcgtcgcggc gctgctcgtt tggctctggg tcggcgcgct acatgacctc gcgctcatcg
     1201 gagtcttgct cggcttacgg gtagaggctc acgcggtgtt cgcgctcgcc gcgctgaatc
     1261 cggtcgaggc cgcgcgtctc gcgctattgt cgagcgtcga cccggagctc tcggtgttgg
     1321 gtccagtcgg cttctggctt tcgaacgagc tcggtgcgaa gcgcacgttg ctcgtcggta
     1381 cgctttggcc gctcgtgctg acggccggca gtttgctcgt agcgttgcgt cgactgcagc
     1441 gcgccgatct cgtaggttga gcccccgacg tctggaagaa tggagaggtc atgtccgtaa
     1501 gaaatcgctt gattgttccg tgtttctcgc tgctcgtcgt gttcggttgc gccaagaagg
     1561 aagcgccgag tcagcaagcg gaaccagccg cggccgctcc ggcgccggcc gccgccgcgc
     1621 cgaccgcaga ggacccagca cgcgaggccc gaaatctatt caagacccgc tgcgtcgtct
     1681 gtcacggcga agagggcaaa ggcgacggcc cgggcgcggc tgcgctcaac cccaagccgc
     1741 gcgactacac cgacgcggcg tggcaggcat cagtgaccga cgagcaaatc cacaagacaa
     1801 tcgtcttcgg tggcgcggca gtgggaaaga gcccgaacat gccgggcaac cctgatctcg
     1861 agagcaagtc ggccgtcgtc gacgagctcg tcaagcgagt gcgctccttc aagaagtgat
     1921 acgcgaagag ccgtgcgcgt cctctatctc gcctcggtgt ggctgcacat cctcgccgcc
     1981 acggtgtgga ttggcggcat ggcttttctc gtgctcgtcg tggtgccttg gctacgccag
     2041 cgaggctcgg cagacgccgg taagctgctg cgcgagacgg gggccaggtt tcgcacggtg
     2101 ggctggagtt gcttcgggat cgtggtgatc acgggcgctt tcaacctgtg gtgccggggt
     2161 gttcggctca gcgactttgc ccgcgccgag tggcttggct cgccgttcgg tcacaccgtg
     2221 ctctggaagc tttcggtgtt tgcactggtg ctcgtgctga gcgccatcca cgactttcgc
     2281 acggggccac gcgcgaccgc cgcgctcgag cgcgacccca cttcgagtga agcgcaacga
     2341 ctgcgccgca tcgcatcgct gttcggccga gtcaacgcgc tgctcggtct cctgctggtt
     2401 gcgatcggcg tggcactggt tcgagggttg ccatggtaag gcctgctacg tccgctgaac
     2461 cgttgattgc gccggcgctc ctgcgtcgaa gcgttcgcac cgagaccgcg cccggcggga
     2521 acgcgtggct gcgaaaaggc ttccgcccgt tcttcgcgct cgcggccgcg tacggcgcgc
     2581 tcatcattcc gctctggctt ctcatcctga atggcctcgt tcgcgtgcct gggcttcgcg
     2641 atccgctgct ctggcatgcc cacgaaatgc tcttcgggta cacgaccgca gtggtcgcgg
     2701 gcttcttgct cacggccgtg ggccgctgga gcggcaaaga gaccgcagtc ggcgggccct
     2761 tgcttgcgct cgccgtgctc tggttgctcg ggcgggtcgc catgttgtcc cccttcgtgc
     2821 cgctcggctt ggcggcgctg ctcgacgcgg cgtttttacc cgcgctcgcg ttcgcagtgg
     2881 ggcgcccgct gatactggcc cgcaatcggc gcaattacgg cattctgggc gtaattctgc
     2941 tgcttgcgct cgcgaacgtg ggcacgcacc tcgacgcgtt cggggtcggg gggtgggcgc
     3001 ggcagagcta cttgttcgcg ctcgacttga tggtgctctt gatggtcgtc gtcggcgctc
     3061 gggtgatacc gatgttcacg cgcaatgcgc tcggtgactc gagcgtgcgc tcggacgtac
     3121 tctgggatcg cgccgcgctc g
//
LOCUS       DASTKS010000202         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1370264, whole genome shotgun sequence.
ACCESSION   DASTKS010000202 DASTKS010000000
VERSION     DASTKS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370264"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFQ35_04265"
     CDS             <1..1007
                     /locus_tag="VFQ35_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET9929873.1"
                     /translation="GERLDARARLSQEIRALSESEPVADGGEAEWLEKLVASSSGDMP
                     AESLRTIFREVRAAGRAIEQPARVAIVGPEGGFCYQSALEAFGATTQTVETASAAEAL
                     SEVIRSRAAFAMIPFESSSEGFSQPSLMALAETDLVLVGERFASATYNLMSLASDKAQ
                     VEKVYMTAAAHAACQRFLDAELPRASIIDVRSPRVAADLAKESNTAAAVVPERAGRAV
                     GLESLRPNVGDVAEARNRYGIAGLRPAPRSGHDISCLLFSTDNQPGSLYDTLRHFAER
                     GINLRKLQSLPARRDGFEYIFYAEISGHASDRAVVTAIEAIKRSVGYFRLLGSFPTAT
                     "
     gene            1048..2193
                     /gene="hisC"
                     /locus_tag="VFQ35_04270"
     CDS             1048..2193
                     /gene="hisC"
                     /locus_tag="VFQ35_04270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9929874.1"
                     /translation="MQPTVKLDSRPDSQFPTATESRHGLVLPTVETLAPYEGGKPLEE
                     LAREYGVTDAVKLASNENPLGPSPRAIEAARGVLDSAHLYPDGAAFRLRERLARAHGV
                     GMDELILGNGSNELLDLFVKAFATREHHIVFAEPSFVVYRISALAYGVPFTAVPLTAL
                     THDLPAMAAAVTPRTRLLFIANPNNPTGTHVGKAAVEKLLREVPEDVIVVMDEAYIEY
                     ADAPDFPDSLTLRKLRERLIVVRTFSKIYGLAGLRVGYAIGPALLINYLNRVRAPFNV
                     STIGQVAAIAALDDVEHVERSRTLNRKERARVSDVLTRAGLDVAPSQANFVLVDVKRP
                     ARPVYEALLRKGVIVRPFASLPTHLRITVGTERENDRLLTALAEALR"
     gene            2190..>3192
                     /locus_tag="VFQ35_04275"
     CDS             2190..>3192
                     /locus_tag="VFQ35_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929875.1"
                     /translation="MKHRRSLRALVVALSVALGLAACAKKQDVIVESGEGKALGAKAI
                     DADPIALLPGNAVGVYTLDAKALVASPFGARLVAIAKARSPLPESSGFDPARDLERVY
                     GGIYSMQGADSVGVAVGQFKPEQIERAAQAAMQPGGSPGNVPITRSEYAGRVFYTSRG
                     LGFSVLTDHTLLIGDETGLRRALDRIQEGRVKRQLPEWMVKTLENKSVPLVFSADFTS
                     QPLPSAARDQLAFVDGLRTVALLGNFADPGLNLAGTLTYGEPDAATRGAQNLQQLHGR
                     LSTFAPLMAMLGIPQPVRSLQADAHEKEVRFVAGVDGGAVAALLERAESYLSSLTPAQ
                     "
BASE COUNT          501 a         1064 c         1075 g          552 t
ORIGIN      
        1 ttggcgaacg cctcgatgct cgcgcccggc tctcacagga gattcgcgcc ctcagcgagt
       61 ccgaaccggt cgcggacggc ggcgaagcgg agtggctcga gaagctcgtc gctagctcga
      121 gcggcgatat gccggcggag agcttgcgga ctatcttccg cgaggtgcgc gcggcgggtc
      181 gcgccatcga gcagccagct cgcgtcgcca tcgtcgggcc cgaaggcggc ttctgctacc
      241 agtcggcgct agaggctttc ggggcgacga cgcaaacggt cgagacggcg agcgcggcgg
      301 aagctctctc ggaggtgatc cgttcgcgcg ctgccttcgc gatgatcccg ttcgaatcgt
      361 cttccgaagg cttttctcag ccgtcgctga tggcgctcgc ggagacggac ttggtgctcg
      421 ttggtgagcg attcgcgtcc gcgacctaca acctcatgtc gctcgcctcc gacaaggcgc
      481 aggtcgagaa ggtgtacatg acggccgcgg cgcacgccgc gtgccagcgc tttctcgatg
      541 ccgagctgcc ccgtgccagc atcatcgacg tgcgctcgcc gcgtgtcgcg gcggatctcg
      601 cgaaggagtc gaacaccgct gccgcggtcg tgcccgagcg cgctggtcgc gctgtcgggc
      661 tcgagagtct gcgcccgaac gtcggggacg tcgccgaggc gcggaatcgc tacggcatcg
      721 cgggtctgcg gccggcgccg cgcagtggcc acgacatctc gtgcctcctt ttcagcaccg
      781 acaaccagcc cggctcgctc tacgatacgc tgcgccactt cgcggagcgc gggatcaacc
      841 tgcgcaagct tcagtcgttg cctgcgcgtc gcgacggctt cgagtacatc ttctacgcgg
      901 agatcagcgg gcacgcatcc gatcgcgccg tcgtcaccgc gatcgaagcc atcaagcgtt
      961 ccgtcgggta cttccgtctg ctcggttcat tcccgaccgc gacctgaacg ggcttgcctc
     1021 gccgggaagc gtggtaggct gcgcgtcgtg cagcccaccg tgaagctcga ttctcgtccc
     1081 gatagccaat ttccaacggc caccgagtcg cgtcatgggc tcgtgctccc caccgtcgag
     1141 acgctcgcac cgtacgaggg aggcaagccg ctcgaggagc tcgcgcgcga atacggcgtg
     1201 accgatgccg tgaagctcgc atcgaacgag aaccctctgg ggccgagccc gagggcgatc
     1261 gaggcagcgc ggggcgtact cgacagcgcg catctctacc cggacggcgc ggcatttcgt
     1321 ctgcgcgagc ggctcgcgcg ggcgcacggc gtcgggatgg acgagctcat tctcggaaac
     1381 ggctcgaacg agctgctcga cttgttcgtg aaggcgttcg cgacgcgcga gcaccacatc
     1441 gtgttcgcgg agccgagctt cgtcgtgtat cgcatctcgg cgctcgctta tggagtgccg
     1501 ttcacggcgg tgccgctcac cgcgctgacc cacgacttgc cggcgatggc ggcggcggtc
     1561 acgccgcgca cccggctcct gttcatcgcg aacccgaaca atccgacggg aacgcacgtc
     1621 ggtaaggcag cggtcgaaaa gctcctgcgc gaggttcccg aagacgtcat cgtcgtgatg
     1681 gacgaggcgt acatcgagta cgccgacgct ccggatttcc cggattcgct cacgcttcgg
     1741 aagttgcgcg aacggctcat cgtcgtccga acgttctcga agatttatgg tctcgccggg
     1801 cttcgcgtcg gttacgcgat cgggccggcg ttgctcatca attacctgaa ccgagtgcgc
     1861 gcaccgttca acgtgagcac gatcggccaa gtcgctgcga tcgccgcgct ggacgacgtc
     1921 gagcacgtcg aacgcagccg aaccctgaat cgcaaggagc gagcgcgcgt gagcgacgtg
     1981 ctgacgcgcg ccggcctcga cgtcgcgccg agccaggcga acttcgtgct cgtcgacgtc
     2041 aagcgccccg cacgcccggt gtacgaggcg ttgctccgta agggtgtcat cgtgcggccg
     2101 ttcgcgagtc ttccgacgca tctgcgcatc accgtcggca ccgagcgcga aaacgatcgc
     2161 ctgctcacgg cgcttgccga ggctttgcgg tgaaacaccg gcgttcgtta cgggcgttgg
     2221 tcgtcgcgct ctcggtggcg ctcggcctcg ctgcctgcgc aaagaagcaa gacgtcatcg
     2281 tcgagagcgg cgaaggtaaa gcgctcgggg cgaaagccat cgacgccgat ccaatcgcgc
     2341 ttttgcccgg caacgcggtc ggtgtgtaca cgctcgacgc gaaggcgctc gtcgcttcgc
     2401 ccttcggcgc tcggctcgtc gcgatcgcca aggcacgctc gccgctcccc gagtcgtcgg
     2461 ggttcgatcc agctcgcgat ctagagcgcg tgtacggcgg catctactcg atgcaaggcg
     2521 ccgacagcgt gggcgtcgcg gtgggacagt tcaagccgga acaaatcgag cgcgcggcgc
     2581 aggcggcgat gcagccgggc ggttctcccg gcaacgtgcc gataacgcgc agcgagtacg
     2641 ccggtcgggt tttttatacg tcgcgcggcc tcggcttcag cgtcctcacg gatcacacgc
     2701 tgctcatcgg cgacgagacc gggctccgcc gcgcgttgga tcgcattcaa gaggggcggg
     2761 tgaagcggca gcttccggag tggatggtaa agacgctcga gaacaagtcc gttccgctcg
     2821 tcttcagcgc cgacttcacg tcgcagccgc tgccttccgc cgcgcgcgac cagctcgcgt
     2881 tcgtggacgg gcttcgcacc gtcgcgctgc ttggtaactt cgcggatccg ggcctgaatt
     2941 tggcgggcac gttgacgtac ggggagccgg acgcggcgac tcgaggcgca cagaacttgc
     3001 agcagctgca tggtcgcttg tcgacgttcg cgccgctcat ggcaatgctc ggcatcccgc
     3061 agccggtgcg ctcactgcaa gccgatgcgc acgagaaaga agtgcgtttc gtcgcgggcg
     3121 tcgacggtgg ggccgtcgcc gcgctgctcg agcgtgccga gtcgtacttg tcgagcctca
     3181 ctccggcgca gc
//
LOCUS       DASTKS010000203         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1147097, whole genome shotgun sequence.
ACCESSION   DASTKS010000203 DASTKS010000000
VERSION     DASTKS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147097"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2347
                     /locus_tag="VFQ35_04280"
     CDS             <1..>2347
                     /locus_tag="VFQ35_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929876.1"
                     /translation="DSDFVQLAKPLLRNHFEKDQLLGWQLCPVDERIQQFLDDYLGNV
                     WPDGAPRLPARTFVLDRPGIARELSLPPSADRFASPYLTSYRIAQGVLHNPKSDRRTT
                     QGVFHVAEGGLPVPVDKQEVPKRAFAALLRAALTPPKDVLTIPFCAEQSRPVELFVSL
                     LLRPIVCPSTGRDPEKRMEVRFFAPGTLVSNLDFVESIFGNGGDPFLPENDAALDCSG
                     WTGHTGCVILAPHLVGLRKKDLGLPHYDHATPRQRRDGMCYRDEAEPYNGGNAFKITC
                     RDTRGVIVTIIADNYYGYCKKEVKTQISYAANLYGACEEEHAGGVIAFPSYVLGNRFS
                     RELVADLGPAKFEEAIALLGARVEVKPGRYAVDKLYPTIVYLPEDAEFNAREGFVRWR
                     DGEQAQERPLRVEETYVLPSGYRVRLEKHPGGTSWRLVGTRAEGVLCHKPCTVSGGGK
                     SEISKSLRPMIHPSPVFVKDFRNDFERVAQITKMDFSKCFKVAPADERARRPLLSPER
                     SLGSVVKLLTPSDNYTDEHNTWLKALPQTIRELVFVVKRNYRHEWGEDFQSHFKVDSV
                     NGYAGHELKFDDQRLLTGQLRVGFEPGSSVWRMHKLRPDFRPADKVQVEDDITASVII
                     PRALGRGLSSEYANQSLKVVTNCEEYLFQRPDDAIHRGFDAQAEADMSTPGTFITNFE
                     PLDRGAAQKLVDRVADLDCFTAPMKSLLTDFVTKGSTDYVVSSAHPRIVDGKPSKNPR
                     YLQHRSDHVDHEQTYVAEISARLNGKISDEQVWLQLVQAVLA"
BASE COUNT          449 a          771 c          725 g          402 t
ORIGIN      
        1 agattcggac ttcgttcaac ttgcgaagcc gttgctccga aaccacttcg agaaggacca
       61 gctactcggc tggcaactct gcccagtcga cgagcgcatt caacagtttc tcgacgacta
      121 cctcggcaac gtttggcccg atggtgctcc gcgtctgccg gcgcgcacgt tcgtcctcga
      181 tcgccccgga atcgcccgtg agctctcgct ccctccgagc gccgaccggt tcgcctcgcc
      241 gtacctgacg tcgtaccgaa tcgcgcaggg cgtgcttcac aacccgaaga gtgatcggcg
      301 cacgacccaa ggcgtgtttc acgtagccga gggcggcttg ccggtcccgg tcgacaagca
      361 agaggtgccg aagcgcgcgt tcgcagcgtt gttgcgcgcg gcactcacgc cgccgaaaga
      421 cgtgctgacg atcccgtttt gcgcggagca aagccgccct gtggagctct tcgtctcgct
      481 gctgctccgg ccgattgtct gcccgagcac cgggcgcgat ccggagaagc gcatggaggt
      541 tcgcttcttc gcgcccggca cgttggtgag caacctcgac ttcgtcgaga gcatcttcgg
      601 gaatggaggc gatccgttcc ttccggaaaa cgacgccgcg ctcgactgca gcggctggac
      661 tggccacacc ggctgcgtga tcttggcgcc gcacttggtc gggctgcgca agaaggattt
      721 ggggctgcct cactacgacc acgcgacccc ccggcagcgc cgcgacggca tgtgctatcg
      781 cgacgaggcc gagccctaca acggcggcaa cgctttcaag atcacgtgcc gcgacacccg
      841 cggtgtcatc gtcacgatca tcgcggacaa ctactacggc tactgcaaaa aagaggtcaa
      901 gacgcagatc agctacgcgg caaacctcta cggcgcgtgc gaagaagagc atgccggtgg
      961 cgtcatcgcc tttccgagct acgtcctcgg aaaccggttc agcagagagc tcgtggcaga
     1021 tctcggcccc gcgaagttcg aggaagcgat cgccttgctg ggggcgcgcg tcgaggtcaa
     1081 acccggccgc tacgcagtcg acaaactcta cccgaccatc gtttatctcc cggaagacgc
     1141 cgagttcaac gctcgagaag gcttcgtccg ctggcgggac ggcgaacaag cgcaagaacg
     1201 acctttgcgc gtcgaggaga cgtacgtgtt gccctccggg tatcgggtgc ggctcgaaaa
     1261 gcatccgggc ggcacgagct ggcgtctggt gggcacgcgc gcggagggcg tcctctgcca
     1321 caagccgtgc accgtgtccg gcggcggcaa atccgaaatc agcaagtcgc tgcgcccgat
     1381 gattcacccg tcgcccgtgt tcgtgaagga cttccgcaat gacttcgaac gagtcgcgca
     1441 gatcacgaag atggactttt cgaagtgctt caaggtcgcg cccgcggacg agcgcgctcg
     1501 ccggccgcta ctgagcccgg agcgttcgct cggctcggtg gtgaagctcc tcacgccttc
     1561 ggacaactac acggacgagc acaacacctg gctcaaagcg ctaccgcaga ccattcgcga
     1621 gctcgtgttc gtcgtgaagc gcaattaccg gcatgagtgg ggtgaggact ttcaaagcca
     1681 cttcaaggtc gacagcgtca acggctacgc gggtcacgag ctcaagttcg acgaccagcg
     1741 cctgctcacg gggcagctcc gagtcgggtt cgagcccggg agctcggtgt ggcgcatgca
     1801 caagctgcgt ccggattttc gaccggcaga caaggtgcag gtcgaggacg acatcacggc
     1861 gtcggtcatc atcccgcgcg cgctcggccg agggctctcg agcgagtacg cgaaccagag
     1921 cctcaaggtc gtcacgaact gcgaggagta cctgttccaa cggcccgacg acgcgattca
     1981 ccgcggcttc gacgcccagg cagaggcaga catgtcgacg ccggggacct tcatcacaaa
     2041 cttcgagccg ctcgatcgcg gcgcggcgca gaagctcgtc gatcgggtcg ccgacctcga
     2101 ttgcttcacc gcgccgatga agagcctgct caccgatttc gtaaccaagg gttctaccga
     2161 ctacgtggtt tcctcggctc acccgcgcat cgtcgacggg aaaccatcca agaacccgcg
     2221 ctatctgcag caccgttcgg atcatgtcga ccacgagcag acctacgtcg ccgagatttc
     2281 ggcccgcttg aacggaaaga tcagcgacga gcaagtctgg ctccagctcg tgcaggcggt
     2341 gctcgct
//
LOCUS       DASTKS010000204         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_254393, whole genome shotgun sequence.
ACCESSION   DASTKS010000204 DASTKS010000000
VERSION     DASTKS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254393"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3151)
                     /locus_tag="VFQ35_04285"
     CDS             complement(<1..3151)
                     /locus_tag="VFQ35_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9929877.1"
                     /translation="MFESTASASDPELPARYEAVRRLGQGGGGEVWAVRDRTSGGIFA
                     LKVLAEDASDREMAALVREAAALSGLEGLGVPRVQGFGRLPRTGRAYMLRELVEGTSL
                     DAWIARGATLESALRALASASDQLTVLHRAGLLHGDVKPANVIVHADGSATFVDLGLA
                     APFREGGAQVEGLSPKYAAPELFEGRPLTARAEVYALGVTLQELLETHDRSISESARA
                     ELARVAEVATSESLNDRYPSADEFASALRRGIGLGPSSEDSSETAALWPVVGIDAISA
                     HISKAADALGESGLLVLTGETGAGRTALLRRLSWSLGARGEPVVLFDRKASASEVDKE
                     LAQYAHLRGVFVLIDDADEFDEAALARLREARVAGARLVVVGKAELGVHDREIEVPPL
                     DTHAARDLVRRAIPSLGDAVLTRVIELSGGRPGELRRIVRLIAKEPLASTTEVERLIG
                     RSEAPPDLPSDPLERALLLLARGRYIDAAAALDELPADVGERAAAVAIARARLDLGLG
                     DAEGAHQKLAPFVSDPQAMRDDLLLYWGRAKLGTCDYAGALEILGALAERESPLGAEA
                     KAFYGLALSLVGRQEEAQSAVGSAVERARALGDARSEAVTLASQGLVEQRADRTDEAR
                     AAYEAAITAAERANDVGTLALVRANLAGLLKVRGDIAGAIEQFEAALDMGRRSGRRQT
                     ARQALLNLANLDVYLGRIARARARIEALEEQRAQLPQLMRAQITGLTAELLLKEGSVE
                     RAVKEYEATAREYEALGLAVDASEARLEAVLAGVRANEPDTHELRRAFELGRTGLGDT
                     KAHRPLLLLAAAGIASAVGDEAEARARLDEALVAAREASQREWLWRALAARAELEETG
                     GQPLLAKRDREEAVAVLEEIGARLPRDLREVYWNDARRNQLRTLVANTLASAATEFSA
                     FSSGALPERAGNNTLSRTTNLSQLSTTPLERRLARLLEVNAELAAEHDLERLTARITD
                     YAVELARAERGLVILRNPDGTLVIKSSRARGADQPDAEFSRSIAEEVMESHKPVVALN
                     ARSDARMKNYRSVHQFSVES"
     gene            complement(3200..>3848)
                     /locus_tag="VFQ35_04290"
     CDS             complement(3200..>3848)
                     /locus_tag="VFQ35_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929878.1"
                     /translation="GKTYNFVGARYRLIVVPKFIIGMFADGGTTVAVHAGGPEFGVRK
                     AGFEFNFALWLAGYGMDPTPFKAKSDGESAWELVESNIKVLYLTSDLLWSHSFSHELA
                     INYGMGAGLGVVWGPLHRNQAYRSGSGFTKCPSNAAGNGPGPGAPGVCGTDNNHYGNY
                     EEPAWTSGGSKPIVFPWLAVQTGLRYKPHRRFVGRLDLGFGLSGFFVGIGADYGL"
BASE COUNT          607 a         1390 c         1247 g          604 t
ORIGIN      
        1 ccgactccac cgagaactgg tgcaccgaac ggtagttttt catacgcgcg tcgctacgcg
       61 cgttgagggc caccaccggc ttgtggcttt ccatcacctc ctcggcgatg gagcgcgaaa
      121 attcggcgtc gggctggtcg gcgccgcgcg cgcgcgagct cttgatgacc agcgtaccgt
      181 cggggttccg caagatcacg agccctcgct cggcgcgcgc gagctctacc gcgtagtcgg
      241 tgatgcgagc cgtgagtcgc tcgagatcgt gctcggcggc gagctcggcg ttcacctcca
      301 agaggcgcgc caggcgccgc tcgagcggcg tcgtcgagag ctgcgagagg ttcgtggtgc
      361 ggctcagcgt gttgttaccc gcgcgctcgg gcagggctcc gctgctgaag gcagagaact
      421 cggtcgcggc gctcgcgagc gtgttcgcca ccagcgtgcg aagctgattt ctgcgcgcgt
      481 cgttccagta cacctcgcga aggtcacgcg gcaggcgcgc gccgatctcc tccagcactg
      541 ccactgcttc ctcgcggtcg cgcttggcga gcagcggctg accgcccgtc tcttcgagct
      601 cggcacgcgc ggcgagcgct cgccacagcc actcgcgctg gctcgcttcg cgcgcggcca
      661 ccaacgcttc gtcgaggcgc gcacgcgctt cggcctcgtc gccgaccgcg gaagcgatgc
      721 ccgcggccgc caggagcaag agcgggcgat gtgccttggt atcgccgagc ccggtgcgac
      781 cgagctcgaa agctcggcga agctcgtgcg tgtcgggctc gttggcgcgc acgcccgcga
      841 gcaccgcctc caggcgagct tcggaagcgt cgacggcgag gcccagcgcc tcgtactcgc
      901 gcgcggtagc ctcgtactct ttcaccgcgc gctcgacgct gccctccttc aagagcagct
      961 cggcggtgag gcccgtgatt tgagcgcgca tgagctgcgg caactgcgcg cgctgctcct
     1021 cgagcgcctc gatgcgcgcg cgcgcccgcg cgattcggcc caggtaaacg tcgaggttcg
     1081 caaggttcag cagcgcctgg cgcgcggttt gacggcgacc ggagcggcgg cccatgtcga
     1141 gcgcggcctc gaattgctcg attgcgcccg cgatgtcacc gcgcactttg agcaggccag
     1201 cgaggtttgc gcgaaccagc gcgagagtgc cgacgtcgtt ggcccgctcc gccgccgtga
     1261 tcgcggcctc gtaggcggcg cgagcctcgt cggtgcgatc ggcgcgctgc tcgaccaacc
     1321 cttgcgatgc gagcgtcacc gcctccgagc gcgcatcgcc gagcgcgcga gcgcgttcga
     1381 ccgcgctgcc caccgcgctc tgcgcttcct cctgacgacc gactagcgac aaggcgagcc
     1441 cgtaaaaagc cttggcttcg gccccgagcg gagactcgcg ctcggcgagc gctcccaaga
     1501 tctcgagcgc gcccgcgtag tcgcacgtcc cgagctttgc gcggccccaa taaaggagca
     1561 gatcgtcgcg catcgcctgc gggtcgctca cgaacggcgc aagcttttgg tgcgcgcctt
     1621 ccgcgtcgcc caagccgaga tcgagccgcg cgcgcgcgat tgcaacggcg gcggcgcgct
     1681 cgcccacgtc ggcgggcagc tcgtcgagcg ccgcggcggc gtcgatgtag cgacctcgcg
     1741 cgagcaacaa gagcgctcgc tccagcggat cgctcggcag atccggcggt gcctccgagc
     1801 gtccgatcag gcgctcgacc tctgtcgtgg aagcgagcgg ttccttggcg atgaggcgca
     1861 cgatgcgccg cagttctccc ggcctacctc ccgagagctc gatgacccgc gtgagcaccg
     1921 cgtcgccgag cgaagggatc gcgcggcgca ccaggtcgcg cgcagcgtgc gtatccagcg
     1981 ggggcacctc gatctctcga tcgtgaacgc cgagctcggc tttgccgacc accacgagtc
     2041 gggccccagc gacgcgcgct tcgcgaagcc gcgcgagcgc cgcctcgtcg aactcgtcgg
     2101 cgtcgtcgat gagcacgaac accccgcgca ggtgggcata ttgggcgagc tccttgtcga
     2161 cctcgctcgc gctcgctttt cggtcgaaca gcacgacggg ttcaccccgc gcgccgagcg
     2221 accacgagag ccgtcgcaag agagcggtgc ggcccgcccc cgtttcgccc gtgagaacca
     2281 acaaccccga ctccccgagc gcgtcggccg ccttcgagat atgcgccgag atcgcgtcga
     2341 ttccgacgac tggccatagc gccgcggtct cggaagagtc ttcagagctc ggcccaagcc
     2401 caatccctcg tcgcaacgcg ctcgcgaact cgtccgccga tggatagcga tcgttcaggc
     2461 tctccgatgt cgcgacttcg gcgacacgcg caagctccgc ccgcgccgac tccgaaatgg
     2521 agcggtcgtg tgtttcgagc aactcctgca acgtgacgcc gagcgcgtac acctccgcgc
     2581 gcgcggtgag cggccttcct tcgaagagct cgggcgcagc gtacttcggg ctcagaccct
     2641 cgacctgagc tcccccttca cgaaacggcg cggccaagcc gaggtcgacg aaggttgcgc
     2701 taccgtccgc gtgcacgatg acgttcgcgg gcttcacgtc gccgtgcagc aggccggcgc
     2761 ggtgaagcac ggtcagctga tccgaagcgc tcgcgagcgc gcgcaacgcg ctctccaacg
     2821 ttgccccgcg agctatccag gcgtcgagac tcgtgccctc gaccaactcg cgaagcatgt
     2881 aggcgcgacc cgtgcgcggc agccgaccga agccctgcac gcgaggcaca ccgagccctt
     2941 cgaggcccga gagcgccgcc gcttcccgca cgagcgctgc catctcgcgg tcgctcgcgt
     3001 cttcggccaa aaccttcaac gcgaagatgc cgccactcgt gcgatcgcga accgcccaca
     3061 cttcaccgcc gccgccctgc cccaggcgac gcacggcttc gtatcgagca ggtagctccg
     3121 gatcgctcgc tgacgccgtg gactcgaaca cgctcaaaac agcccgcctt ttcccctcgg
     3181 ctccgttcga gccgtgccct cagagtccgt agtcggctcc gatgccgacg aagaacccac
     3241 tcaggccaaa cccgagatcg aggcgcccca cgaaccgacg gtgcggcttg taacgaagcc
     3301 ccgtttgaac ggcgagccac ggaaatacga tcggcttcga accgccgctc gtccaagcgg
     3361 gctcttcgta gttgccgtag tgattgttgt cagtgccgca cacaccagga gcaccgggcc
     3421 caggaccgtt acctgccgcg ttgctcgggc acttggtgaa gccactgccg ctgcgatacg
     3481 cctggtttcg gtggagggga ccccaaacca cgccgagacc ggcgcccatg ccgtagttga
     3541 tcgcaagctc gtgagagaac gaatggctcc agagcagatc cgacgtcagg tagagcacct
     3601 tgatgttgct ctcgacgagc tcccacgcgc tttcgccgtc gctcttggct ttgaacggcg
     3661 tcggatccat tccgtacccc gcgagccaga gcgcgaagtt gaattcgaag ccggccttgc
     3721 gaacgccgaa ctctggtccg ccggcgtgca cggcgacggt cgttccgcca tcggcaaaca
     3781 tgccgatgat gaacttcgga accacgatga gccggtagcg cgcgcccacg aagttatagg
     3841 tcttgccc
//
LOCUS       DASTKS010000205         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_32938,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000205 DASTKS010000000
VERSION     DASTKS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32938"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFQ35_04295"
     CDS             <1..318
                     /locus_tag="VFQ35_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929879.1"
                     /translation="ACALFEVGQLDASGAVKIDPGGVVNPPEAVNPELLDAPKLLWIG
                     GGAAIALDVALGRRLTLEVAATSLIVAKRARFVEASAAGTQLNEVPRAIVGLSITALT
                     WIY"
     gene            592..1191
                     /locus_tag="VFQ35_04300"
     CDS             592..1191
                     /locus_tag="VFQ35_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929880.1"
                     /translation="MTALGLTAIATPSAASDVDCDEAEEFHVSQADAERIRAALENEH
                     ELVWRSLRRFGVDPTLVDDATQSVFVTFARRIRDVTVGRERSFLLGVCCRTAANLRRL
                     VARRREVPGDPLVQEPDDELNPEELLDYKRRRRLLDLALEDLPTEQRTVFVLYELEGW
                     TLPELAESLQLPLGTVTSRLRRARARFERWVLQHDGGVR"
     gene            1197..>2017
                     /locus_tag="VFQ35_04305"
     CDS             1197..>2017
                     /locus_tag="VFQ35_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929881.1"
                     /translation="MPRLLDTGPPEVRELLAVGLEDAPGKHLLPALRSALGASLVVSG
                     AAVAGSAATSATSMVASASTSGTFAKSTATGAAVVAKTSVSLIAAKALASGLLVGSAL
                     CGGAILTGVGQRPALVHRSARSELSAPSAEKGSPASHAQRQLGAAAPFEAPNQTQVLP
                     RNAPGSEPLPLVEAELAEAPSALQRSRKQRDAGNGKRTHAEPASTAVTSGEALPVLPA
                     EAPAPKPTAQPEAPNLLALEIARVDEARALLNSGKPDAALAALKSYDPTSPSAVLG"
BASE COUNT          334 a          661 c          684 g          338 t
ORIGIN      
        1 gcatgcgctc ttttcgaggt ggggcagttg gacgcttcgg gcgcggtaaa gatcgacccg
       61 ggcggcgtgg tgaaccctcc cgaggcagtc aaccccgagc ttttggacgc tccgaaattg
      121 ttatggattg gtggcggtgc cgccatcgct ctcgatgtgg cgctcgggcg acgcctcacc
      181 ctcgaagtcg cggcgacgtc gctcatcgtc gctaagcgcg cgcgattcgt cgaggcgagc
      241 gcggcgggca cgcagctgaa cgaagtgcca cgagcgatag ttgggctttc gatcaccgcc
      301 ctcacttgga tctactgacg agcgcggggg cgctttttcg gtgactcgac acgctcacga
      361 cttcgattgg tgagaattgc cagcgagatc agtcgaccac cgatacaagt tcgcgacgag
      421 cttcagcgaa tcggtctttc ggtccgggcg ctgaccccga tttttggcga acccgaggat
      481 cgatcccttc gtggctggac atcttggggg gacagtgagt agctgcgtct cacctcgact
      541 ggatgagtct ttgggccgtc gccaagcgct catcgggtgg cggaatccgc tatgacagcg
      601 cttggactga ccgcgatcgc gactccgagt gcggcttcgg atgtcgattg cgatgaagcc
      661 gaagagtttc acgtttcgca ggcagatgcg gaacggattc gcgcggctct cgaaaacgag
      721 cacgagctcg tctggcgctc gctgcgacgc tttggggtcg acccaacgtt ggtggacgac
      781 gccacgcaaa gcgtgttcgt cacgttcgca cggcgaattc gcgacgtaac ggtcggacgc
      841 gaacgctcgt tcttgctcgg cgtatgttgc cgcaccgctg ccaacctgcg gcggctcgtc
      901 gcacgacggc gagaggtacc tggcgatccg ctagttcaag agcctgacga cgagttgaac
      961 ccggaggaac tcctcgacta caagcgccgg cggcggctgt tggacctcgc gttggaggat
     1021 ctgccgaccg agcagcgcac ggtattcgtg ctgtacgagc tcgaggggtg gaccctcccc
     1081 gagcttgcag aaagcctgca gctgcccctg ggaaccgtga cgtcgcggct gcggcgcgcc
     1141 cgggcgcgct tcgagcgctg ggtgctgcaa cacgatggtg gtgtacgatg aaaggcgtgc
     1201 cgcgcctgtt agacacaggg ccgcccgagg ttcgcgaatt gctcgcggtc ggcctcgagg
     1261 atgcgcccgg caagcatctg ctgcccgcgt tgcgctcggc gctgggggca tcgctcgtcg
     1321 tcagcggcgc ggcggtcgcc gggagcgccg ccacttccgc gacctcgatg gttgcgagcg
     1381 cgagtaccag cgggacgttc gccaagagca cggccaccgg cgctgccgtc gtggcgaaga
     1441 cgtccgtgtc gctcatcgca gccaaggccc tcgcctcggg gctcttggtc ggcagcgcgc
     1501 tctgcggtgg agcaatcctg accggcgtcg gccagagacc cgcgctggta catcgctccg
     1561 cgcgctcgga gctgagcgcc ccgtcggccg agaaaggctc gcccgccagc catgcgcagc
     1621 gccagctcgg tgccgcggcg ccgttcgaag cgccgaacca gacccaagtt ttaccgcgga
     1681 acgctcccgg gagcgaacct ctgccgctcg tagaagccga actcgcggag gccccgtccg
     1741 cgttgcagcg ctcgcgaaag caacgagacg ccggcaacgg taagcgcacg cacgccgagc
     1801 ccgcaagcac ggccgttaca tccggtgaag ccctgcccgt actgccagct gaagcaccgg
     1861 cgccgaagcc gaccgcccag cccgaagcac ccaacctcct cgcgctcgaa atcgcgcggg
     1921 tcgacgaggc gcgcgcgctg ttgaactcgg gtaagccgga cgcggctctc gcggcgttga
     1981 agagctacga cccgacgagc cccagcgccg tcctggg
//
LOCUS       DASTKS010000206         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1594239, whole genome shotgun sequence.
ACCESSION   DASTKS010000206 DASTKS010000000
VERSION     DASTKS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594239"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2906)
                     /locus_tag="VFQ35_04310"
     CDS             complement(<1..2906)
                     /locus_tag="VFQ35_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9929882.1"
                     /translation="MPKTIAVLIDHADYPVGGYESQLRAGFEGVCKRLGLNLALFFGR
                     ALDAPLYSAQNAIYELARPDCVDGVVLVAAGLGTFTGAQGVTALAQRLSPLPLVSLGL
                     RIPGVPSIVVDNEPGIHALLDHLIEVHQCRTPAYIGGPELNPDAALRSRAFRDALARH
                     GLPFEPLRFHAGDFTLPSGSSAMATILERGVEFDSVIAANDGMALGAIEMLKSRGVRV
                     PHQVRVCGFDDLEIARLVNPPLTTVRQPLSEMAARAAELLYAQMQGIRVPENNELGVE
                     LVRRGSCGCSRFTRASSPGVRPSQKPASIVVAERAERLVARIARQLHEAGEAAREMAL
                     TMVKGVRDQLDGQPNAFELALEGVIDQVGDRRELYDQLQDVISLLRVELADCGPPAEG
                     LWHEGRALLALASTRASGRQRLHTEIQHQHLVRVGERLLTAFDLPTLKRILAEELPEM
                     SVLNALFALYQDGRKAELAPFLYLRGGVPVELPADKYPATKLVPEGVDVGRRGSTFFV
                     WPLAAEQEVFGIAVIEVTELTNAPEMLREQIAAALRSAALHREIVHRTQLHERSVQER
                     LATAKRMSALNVLAGGVAHDLNNALGPLVALPDLIVRELELVAQNPGLSVNQACSDVL
                     AIKAAALRAVQTIRDLLTSARQGRTQKDVIDLNRSVEALLQSEAPALSASEGIEVSVN
                     LSREPLYVLASESHLQRALSNLLRNAAEAIDPPGRISVRTAQVEVLEPYMGYETVEPG
                     EYAMLAITDTGRGIAHQELGRIFEPFFTKKRVGENSGSGLGLAIVHGVVKEHGGFVNV
                     ESEPGRGTTFTLFLARSKPPARAIETPPDVPHEHGKRILVVDDDPMQLRTARRVLERS
                     GHTVTPYASGREALALFESAHQSSAQTSPFDLVVLDMLLNEADDGLVLAERITALFPD
                     QRCVIVSGHAPTERGLRAVERGLTWLSKPYTADQLTRTVDAAFGPKPEESANSEA"
     gene            4145..>4657
                     /locus_tag="VFQ35_04315"
     CDS             4145..>4657
                     /locus_tag="VFQ35_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929883.1"
                     /translation="MSSVSRWVRVSCAISVGVPLAAIACSHDKPAETGGYQQGQTQPN
                     GYPVAGWGGQPAVQPTTPGGAYGAPTGYGQPTATVPVTPPAAGGVPGTVPPPATPAAG
                     APAASGTAQPLDAAAASVVAPVMNGLAKSETVAGAKPVGAPLVGNFQTGQTLTGQVQL
                     QPNKCYTVVAT"
BASE COUNT          756 a         1579 c         1538 g          784 t
ORIGIN      
        1 gcttcggaat tggcgctctc ttcgggcttt ggcccgaacg ccgcgtcgac cgtgcgagtc
       61 agctggtcgg cggtgtaggg cttgctgagc catgtgagcc cacgctccac cgcgcgcagg
      121 ccccgctcgg tcggtgcgtg gccgctcacg atgacgcagc gctgatctgg aaacaacgcc
      181 gtgatgcgct cggcgagtac caggccgtcg tccgcttcgt tcagcaacat gtcgagcacg
      241 accagatcga atgggctcgt ctgagcgctg ctttggtgcg cgctctcgaa cagcgcgagc
      301 gcttcgcggc cgctcgcgta cggcgtgacg gtgtgcccgc tgcgctcgag gacgcggcgc
      361 gcggtccgca gttgcatggg gtcgtcgtcc acgacgagga tccgcttgcc gtgttcgtga
      421 ggtacgtcgg gcggtgtctc gatcgcgcgc gccggcggct tcgaccgagc gaggaacagg
      481 gtaaaggtgg tgccgcgccc gggctcgctc tccacgttga cgaagccgcc gtgctccttc
      541 acgacgccgt gcacgatcgc gagcccaagc ccgctgccgc tgttttcacc cacgcgcttc
      601 ttcgtgaaga acggctcgaa gattcgcccg agctcctggt gcgcgatgcc gcggccagtg
      661 tcggtgatcg cgagcatggc gtactcaccg ggttcgaccg tctcgtaacc catgtacggt
      721 tcgagcacct cgacctgggc ggtgcggacc gaaatgcgac ccggagggtc gatcgcttcc
      781 gcggcgttgc gcagcaagtt cgagagcgcg cgctgcaggt gcgactccga cgcgagcacg
      841 tagagcggct ctcggctcaa gttcacgctg acctcgatgc cttcgctcgc ggagagcgca
      901 ggggcctcgc tctgaagcaa ggcttccacc gagcggttca ggtcgatgac gtctttttga
      961 gtgcggcctt ggcgcgccga cgtcaacaga tcgcggatcg tttgcactgc gcgcagcgcc
     1021 gccgccttga tcgcgagtac gtcggagcag gcttgattta cgctgagtcc agggttttgg
     1081 gcgacgagct cgagctctct cacgatgaga tcagggagcg cgacgagcgg cccgagggcg
     1141 ttgttcaaat cgtgggcgac gccgcctgcg agcacgttga gcgcgctcat ccgcttggcg
     1201 gtcgccaggc gctcttgcac gctgcgctcg tggagctgcg tgcgatgaac gatttcgcgg
     1261 tgcagcgcgg cgctccgcag cgcggccgcg atttgctcgc ggagcatctc tggagcgttg
     1321 gtaagctccg tgacctcgat gacggcgatg ccgaacacct cttgctctgc cgccaacggc
     1381 cacacgaaga aggtgcttcc acggcgcccc acgtccacgc cctctggaac gagcttcgta
     1441 gccgggtact tgtcggcagg gagctccacc ggcacgccac cccgaagata gaggaagggg
     1501 gcgagctccg ccttgcgccc gtcttgatag agcgcgaaga gcgcgttcag caccgacatt
     1561 tcgggcagct cttcggccag gatccgcttc agcgtcggca agtcgaacgc cgtcagcaag
     1621 cgctcgccga cgcgcaccag atgctggtgt tggatctcgg tgtgcaggcg ctgccgcccg
     1681 ctcgcgcgcg tgctcgccaa cgccagcagc gcgcggcctt cgtgccacag cccctccgcg
     1741 ggcggcccgc agtcggcgag ctcgacgcgt agcagtgaaa tcacgtcctg aagttggtcg
     1801 tagagttcgc gccggtctcc gacttgatcg attacgccct cgagcgcgag ctcgaaagcg
     1861 ttgggctgtc cgtcgagctg atctcgaact cccttgacca tcgtcagggc catctcgcgc
     1921 gcggcctcgc ccgcctcgtg tagttgccgt gcgatgcgcg ccacgagccg ctccgcgcgc
     1981 tcggcgacca cgatcgacgc tggcttctgg ctcgggcgca cgcccggcga tgacgcccgc
     2041 gtgaagcgcg aacagccgca cgagccgcgc cgcacgagct ctacgccgag ctcgttgttc
     2101 tcgggcacgc ggatcccctg catctgagcg tacaagagct cggcggcgcg cgcggccatc
     2161 tcactcaacg gctggcgcac cgtcgtgagc ggcgggttca cgaggcgcgc gatctccaag
     2221 tcgtcgaagc cgcaaacgcg cacctggtgt ggcacgcgaa cgccgcgcga tttcagcatc
     2281 tcgatcgcgc cgagggccat gccgtcgttt gccgcgatga ccgaatcgaa ctcgacgccg
     2341 cgctccaaga tcgtcgccat cgcgctcgat cccgacggca gcgtgaagtc cccggcgtgg
     2401 aagcgcaacg gctcgaacgg aagcccgtgt cgtgcgagcg cgtcgcggaa cgcccgcgac
     2461 ctgagcgctg cgtccggatt cagctccggg cccccgatgt acgccggcgt gcggcattga
     2521 tgcacctcga tcaaatgatc gagcagggca tgaatgccag gctcgttgtc gacgacgatc
     2581 gaaggaacac cagggatgcg caggccgagg ctcacgagcg gcaaaggaga cagccgctgc
     2641 gcgagcgcgg tgacgccttg agctcccgtg aatgtgccga gccccgctgc caccagcacg
     2701 acgccgtcga cgcaatccgg gcgcgcgagt tcgtaaatgg cgttctgcgc gctgtagagt
     2761 ggagcatcga gagcgcgccc gaagaacagc gcgaggttca agcccaaccg tttgcacacg
     2821 ccttcgaagc ctgcccgcag ctgactctcg tagccgccca ccgggtagtc ggcgtggtct
     2881 atgagaacgg caatggtctt gggcatcgtt ccggtcgtgt cggcaacgcg cacggctcga
     2941 gcgcagtgcg cgctggggtt caaaccaacg aagctagcag tgtttgccct cgcttcaata
     3001 cggccatgat gtcgcgcact ccctcgggag tagccattgt aaggtcacta cacccaagct
     3061 cgcgggtgaa tggaatcagg tcagacaccg agcgacagat caggccaaag gactgcgaga
     3121 tttgcgtcct tgcccaacgg ggccgctggt tttgcggtcc gttcgagcgt caccggtgta
     3181 gcagacttcc cgatgcgcca gtcgttcgtg ccgaactcga aacacgatgc gacggcaggg
     3241 cacgacgaca cgacacacac gccttcgtca ggtcgctctg cccgttcttt tcgtgggggc
     3301 ctcgctgaaa agggcgcgca aggcgaccct ggatgcggca tccaggcctg agctcgctca
     3361 gcgggtccag agccgcgccc gccccgcgga gttggttcac gccgccgggt aagggcggtt
     3421 cgtaaagcac tcgtccaaca cgccatgccg acgaaatctt ggcggggttc gcaggtcacg
     3481 gctcgtgcgc atttgagcag cgtcgctagg gcagcgcgtg tgggatggcg tcaacttctc
     3541 cagccgctcc acttgcggcg cacacgaagc ggatgctccg gtctcgggcg gagacccggc
     3601 cacgacaact tgggaaggtg cacgctgcgc gcgggacaag cattgtccgt gatggctgcc
     3661 gtagctcata cgcggtgcac ggcaccggcg cccactcgga acgaccgcaa gattcgcgat
     3721 ccactgccgg cgacgaccgc aaatctcgcg gtccttttgg gcgatccgtc gccaggtgtt
     3781 tggtctcgtt cgacacaccg gagcgagctc gcctgaagaa ctcgtggctc ctcgccggag
     3841 cccgtcgctt cgagcgacga gaatgaaacc gcgtgcagga ccacctcagt tcggcgcgac
     3901 gcaagctcgc gcgctgaccc tggcgacggc tcgacgctgc aatggccggc tctggtcctc
     3961 acgtcccgcg cgacggacca tgttgcaagc ccagcgcacg gtgccgtcgc ccgccgcgcc
     4021 cgcgagatct gatgagcggt caggccgatg ccccacaatg ggcgccattg ggaatccgcc
     4081 gttgcctccg cgcgcctgac cgggctactg atcggctcgt cgcccttggc gagaaggagt
     4141 ccgcatgtcg tctgtgtctc gatgggttcg cgtcagctgt gcgatttcgg tcggcgtccc
     4201 gctcgccgcg atcgcttgtt cgcatgacaa gcccgctgag accggcggct atcagcaagg
     4261 tcaaacgcaa cccaacggct atccagttgc cggctggggc ggacaacccg ccgttcagcc
     4321 aacgactcct ggcggtgcct atggcgctcc taccggctac ggccagccta cggcgaccgt
     4381 gccggttacg cccccggccg ccggcggcgt tcccggaacc gtgccgccgc ccgccacgcc
     4441 cgctgcgggc gctccagccg cgagcgggac ggcgcagcct ctcgacgctg ccgccgcgag
     4501 cgtcgttgcg cccgtcatga acggtctcgc gaagagcgaa acggtcgcag gggccaagcc
     4561 ggtcggcgcg cctctcgtcg gcaacttcca aaccggtcaa accctgaccg gccaagtcca
     4621 gcttcagccc aacaagtgct acacggtggt tgcgacc
//
LOCUS       DASTKS010000207         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_33320,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000207 DASTKS010000000
VERSION     DASTKS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33320"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1159)
                     /locus_tag="VFQ35_04320"
     CDS             complement(170..1159)
                     /locus_tag="VFQ35_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929884.1"
                     /translation="MGKKTKMTHHRWWVLATSVLLAGCGEDTDRYETLDRLRVLAIRS
                     EAPDLAVGETAKLSARVYEPTEGPLRYEWSWCPSRGDADVAFACNIPEQELARAWVAA
                     ELDGEPPTYALGTEREAELTHMLTPELVTALCQAPDLDERIQTACFEGFEASVQLTVS
                     STEAQVTAIKSVPLLMDEEAERNRNPTSDFTLSVSDKDGSGAISEGEPLRAGHQYVVT
                     ANIDEGVAESFMPAAREGEPPPRERRETLVMSWFITVGELDDPDGDDGELNDDFVRTT
                     FVDGRNTVEDLARNGWKVPLTAGPSAELHVVLRDERGGVGWATRRFEVVGGEP"
     gene            complement(1164..2153)
                     /locus_tag="VFQ35_04325"
     CDS             complement(1164..2153)
                     /locus_tag="VFQ35_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9929885.1"
                     /translation="MSQSVNWGAPSERVRPDVARFSRARNAKRQARRELIHARVVHPI
                     DTPRLSFDPLERRESRALSALSAVRLVVSAVMIHVAVILVFAVVGYLLRRERTIHAPE
                     HLTVKIVETQRPVLPPPVAEEPPVSPAPIVPEFESVTPPPKRRDAPVREPPKPRDTPS
                     PAPEPQAAPRRIVGLSLESTVEGSGPAFATGTSRLGRTDPQAVDPLQARRAPAASAPA
                     AAPQPDGTQRIASHIPTRDTQFEKPKRLKPNRPEFPAELKAQGIEGDVTVRVDIAASG
                     AVTSVTIVQSSGYPAFDEAARRAAASERFAPAVRDGNAVPFSLSYSYRFRIED"
     gene            complement(2156..2566)
                     /locus_tag="VFQ35_04330"
     CDS             complement(2156..2566)
                     /locus_tag="VFQ35_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9929886.1"
                     /translation="MAAKVFEDDEEVIAEINIVPFVDIVLVLLITFMLTSSVIAKASF
                     TVELPTAASAGSAVNSTVNVVIDANGKLFLDGAETTDEKLGAFVARASWKDKDVQAVI
                     SADKKVDYGHVVRVIDVIKANGIKSFALNIQREL"
     gene            complement(2573..3241)
                     /locus_tag="VFQ35_04335"
     CDS             complement(2573..3241)
                     /locus_tag="VFQ35_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9929887.1"
                     /translation="MPQESSNLVSMLLGLPIFQAEWVLWVLIGLSFVSVGIMAERFIF
                     YRKHAIDASAVRKKFAELLAAGNMQGAADYLSDYDSLETNTVLFGLRDYARGPEAVED
                     LLAAAARKERARYELRLNFLATIASNAPFVGLFGTVLGIIRAFKDLSGNIAEASSAVM
                     GGIAEALIATAVGLLVAIPAVVAFNLLKAKVKHIADDANLLAATLLASLKSVDLHRHP
                     QAAE"
BASE COUNT          560 a         1101 c         1158 g          597 t
ORIGIN      
        1 ccctgctcga gcgccgccgg cgggtactcc ggctgcacgt ctttgcgcag cttgggcggc
       61 acgaggggct ccggtgtggg ttcgctcggc ttctcggcgc tctgggcccg gcagggcgag
      121 gagcaggtgc cgagcagcag cgcgcaggcg aggagggaat gagctctgtt cacggctctc
      181 ctccaaccac ttcgaagcgt cgcgtcgccc agcccacgcc gccgcgctcg tcgcgcagca
      241 cgacgtgaag ctccgcgctc gggccagccg tgaggggcac cttccagcca ttgcgagcga
      301 gatcctcgac ggtgttgcgg ccgtccacga acgtcgttcg cacgaagtca tcattcagct
      361 cgccgtcgtc accgtcgggg tcatcgagct ctccgaccgt gatgaaccaa ctcatcacca
      421 aggtttcgcg acgttcgcgc ggcggtggct cgccctcgcg cgcggccggc atgaacgatt
      481 cggcgacgcc ttcgtcgata ttcgcggtga cgacgtattg gtgaccggcc cgcaacggct
      541 ctccctcgga aatggcgccg ctcccatcct tgtcgctcac ggaaagggtg aagtcggagg
      601 tagggttgcg gttgcgctcc gcctcctcgt ccatcaggag cgggacggat ttaatcgcgg
      661 tcacctgtgc ctcggtgcta cttacggtca actggacgct ggcctcgaac ccctcgaagc
      721 acgccgtttg gatgcgctcg tccaaatccg gggcctggca cagagccgtc acgagctctg
      781 gcgtgagcat gtgggtcagc tcggcctcgc gctcggtacc gagcgcgtaa gtcggtggct
      841 cgccgtcgag ctcggccgcc acccaggcgc gggcgagctc ttgttcgggg atgttgcagg
      901 cgaaggcgac gtcggcatcg ccgcgcgacg gacaccacga ccactcgtag cgtagcggcc
      961 cctcggtggg ctcgtagact cgagcgctca gcttcgccgt ctcgcccacg gccaggtccg
     1021 gagcctcgga acgaatggcg agcactcgaa ggcggtcgag cgtctcatag cgatcggtgt
     1081 cctccccgca gccagcgaga agcacactcg tagcgagcac ccaccagcgg tggtgcgtca
     1141 tcttcgtttt cttacccatg acttcagtct tcgattcgga atcggtagga gtacgagagg
     1201 ctgaaaggga ccgcgttgcc gtcgcgcacc gccggcgcga agcgttcgct cgcggcggcg
     1261 cgccgggcgg cttcgtcgaa ggcggggtac cccgagctct gcacgatggt gacgctcgtg
     1321 accgcgccgc tcgccgcgat gtccacgcgc accgtcacgt cgccctcgat gccttgcgcc
     1381 ttgagctcgg ccggaaactc cgggcgattt ggcttcaggc gcttgggctt ttcgaactgc
     1441 gtatcgcgcg tggggatgtg actcgcgatg cgctgcgtgc cgtcaggttg aggcgctgcg
     1501 gccggagccg atgccgccgg agcgcgccga gcttgcaacg gatcgaccgc ctgcgggtcc
     1561 gtgcggccca agcggctcgt gccggtggcg aacgcaggtc cgcttccctc cacggtcgac
     1621 tcgaggctca agccgacgat gcgccgaggt gccgcctggg gttcgggcgc tggggagggg
     1681 gtgtctcgag gcttgggcgg ctctcgtacg ggcgcgtccc gtcgcttggg gggcggcgtg
     1741 acggactcga actccggcac gatcggcgcg ggcgaaacgg gcggttcctc ggccacgggt
     1801 gggggcaaga cgggccgctg cgtctccacg atcttcacgg tcaagtgctc gggcgcgtgg
     1861 atggtacgct cccgacgcaa gaggtaaccc acgacggcga agaccaggat caccgccacg
     1921 tgaatcatca ccgcagagac gacgagccgc accgcggaga gcgcggagag cgcgcgggat
     1981 tcgcgccgct cgagcggatc gaacgagagc cgcggcgtat cgatggggtg caccacgcgc
     2041 gcgtggatga gctcgcgccg agcttgccgt ttggcattgc gagcgcgcga gaacctcgca
     2101 acgtccggcc gtacgcgttc cgacggcgcg ccccagttga ctgactgcga catgctcaaa
     2161 gctcgcgttg gatgttcagc gcaaaactct tgatgccgtt ggctttgatg acgtcgatga
     2221 ctcgaacgac gtgcccataa tccacctttt tgtcggcgga gatgaccgcc tgcacgtctt
     2281 tgtccttcca ggaggcgcgc gccacgaagg cgcccaattt ctcgtccgtc gtctctgcgc
     2341 cgtccaggaa cagcttgccg ttggcgtcga tcacgacgtt gaccgtactg ttcacggccg
     2401 agccagcgct ggcggcggtc ggaagctcca ccgtgaagct cgccttggcg atcacggacg
     2461 acgtgagcat gaaggtgatg agcagcacga gcacgatgtc cacgaacggg acgatgttga
     2521 tctcggcgat gacttcctcg tcatcttcga agactttggc ggccatgttc gctcactccg
     2581 cggcttgggg gtgacggtga aggtcgacgg acttgagcga cgcgagcaac gtcgcggcca
     2641 agaggttggc gtcgtcggcg atgtgcttca ccttggcttt gagcaggttg aaggcgacca
     2701 cggccgggat cgcgaccaac aaaccgactg cggtcgcgat cagcgcctcg gcgataccgc
     2761 ccatcaccgc ggagctcgcc tccgcgatgt tgcccgagag gtctttgaag gcgcgaatga
     2821 tgccgagcac cgtgccgaac aggccaacga acggcgcgtt gctggcaatg gtcgcgagga
     2881 agttcaagcg cagctcgtag cgggcgcgct ccttgcgggc ggccgcggcc aacaagtcct
     2941 ccaccgcttc gggcccgcgc gcgtagtcgc ggaggccgaa caggaccgtg ttggtctcca
     3001 ggctgtcgta gtcggacagg tagtccgccg cgccctgcat gttgcccgcg gctaacagct
     3061 ccgcgaactt cttgcggacg gcgctcgcgt cgatcgcatg tttccgatag aaaatgaagc
     3121 gctcggccat gattccgacc gaaacgaagg acaggccgat gagcacccag agcacccact
     3181 ccgcttggaa gatgggtaaa ccgagcagca tactgacgag gttggacgac tcttgaggca
     3241 tgaacttcct gacgccaaaa gtagggggtt gccgccggcc cttccaccgc tgtttcgctt
     3301 cgttaccttc tgttacaaac caccgctgga cagcgcacgg gctcagcacg aggatgggta
     3361 acgtcatgga tccgcacggt gcgcgagtgc tgctggtcga ggacgacgaa gagctc
//
LOCUS       DASTKS010000208         6973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1147716, whole genome shotgun sequence.
ACCESSION   DASTKS010000208 DASTKS010000000
VERSION     DASTKS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147716"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFQ35_04340"
     CDS             complement(<1..189)
                     /locus_tag="VFQ35_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929888.1"
                     /translation="MGGSIERLLVATRKGLFTVQRTGSGWAVGDVAFEGDDVSMLTYD
                     SASGDIYVAVRRGSECVLA"
     gene            343..933
                     /locus_tag="VFQ35_04345"
     CDS             343..933
                     /locus_tag="VFQ35_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929889.1"
                     /translation="MAARRNSIPSDVHATDEDSLPGSEDLGRLALRAAAGDARAFELL
                     VEQTSDTLVRLGARLMGSLPDGEDVVQEAYVKAYRGLIEGRFDGRAQVRTWLGRIVVN
                     TAIDALRVRKRAPSPRDEAYEAVDASASAETLVALAELDDWLSALPPEQRAAVVLKSI
                     EGHTSAEVADLLGCSEGAVEQKLVRARAALRQRMKS"
     gene            930..1202
                     /locus_tag="VFQ35_04350"
     CDS             930..1202
                     /locus_tag="VFQ35_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929890.1"
                     /translation="MTPEDVEARLARLARQTSAISARAGFSRGVVARVGAQAARSFEQ
                     QLARSARPVVVAAALLALAVLALSLSSQQSLDRSLATEVGAPGAEW"
     gene            1196..1603
                     /locus_tag="VFQ35_04355"
     CDS             1196..1603
                     /locus_tag="VFQ35_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929891.1"
                     /translation="MVIPTRLRVYGAFAAVFLLGAAAGASAAHAYVTRSLLGAEDAGE
                     LRDRRRLEALRRELGLDREQSDHIWTILSRRRPGHRALVRAAFERCGNELRDEREKLD
                     TEIRKELKPSQQQRFDELVRTAHERFPLGREPR"
     gene            1666..3678
                     /locus_tag="VFQ35_04360"
     CDS             1666..3678
                     /locus_tag="VFQ35_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9929892.1"
                     /translation="MGTRAGLQHHSRLRVPKWLALIALSFAPRLAHADPTPEVVVEGE
                     RAQPEAPNRAREVAGSVVRGERLRAPGAGTKEVLRSEPGVQLVELGGLGAPATASLRG
                     ATATQTPIYLAGVRLNDEVGGTANLSDVPAFMLDRIEVYRSHAPLAGDQQGIGGAIYL
                     EPRRVVPNERRAALGGMVGSYGSRSLSAFGGVSGAESNVAAGVEFAATANDYAFYSGN
                     GTIFTPGDDRSARLPNADVSSTSVWVAAAHDTPKARLRLFFHTANREQGAPRFALSPT
                     YHPRVRLSRQLLALTATVPLDSLNGSVELSTSGISSTTRIDDPGNDLTLARPHVATPG
                     ERVEQSVLAREATRGGFRFVEQLTVSEERLRRFEGSASADAEQVAAERRRARLSVGAE
                     APIATGGRVFVNGVVGCLGTTALGRPSCDWEPVTGRLGAAYRVGNFELSANLGSYTRP
                     PTLSELYGTSLLVRGNDELSPEHGTTLEALIRYQLVREGRRLLFFDVSAFGRKSRDLV
                     TFLRTSQNYFVPVNRAKTRVLGSEFIVGATPLAGLEVSGNASLLDPRDESPDRAPAND
                     MLPFLSRLTVGAAVSGRHALGGPKLGTLALTLRATYQSSRYADFAGQGVIPEQASVDV
                     EAGLLEVARLLTTRLRVSNLFDARRFDVVGFPLPGRSLFLSLELGL"
     gene            3675..4916
                     /locus_tag="VFQ35_04365"
     CDS             3675..4916
                     /locus_tag="VFQ35_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929893.1"
                     /translation="MKKILLPLCAAVACNVENTTPDTGGVGVAGAGGCSPGISVVLSD
                     YASTQVALSTLEGQTLSRSFISTGSAKTDGLSFALSGDVVLPSSRPASGRVVLVDRFG
                     TNVLTWLDPATARVLGQLAVGTGFDSNPSDYLELDDQFAFVSRYGQNPEPGRQPFDIG
                     GDLLLVDSRAQEIVGSLPFEATEDLPPRPSGMMRLGDEILVSLVRNARDFSTTGDSEL
                     AGVSVSTRRLDFRMRYEGLKHCGRPALSPSGDVLAVACTGALDIRGVVADVSQSALLL
                     LDARELPPRELRRFSAAELGNQPIQSSATFARDALLLLKTQTSTVGTENNRWLALDLE
                     SGKVTTLLEASPDAQGKGRGVLYGGMSCSPGCSEVCLLADADRGALQRVRFDPLGAPE
                     LLPPIVVDAKSGLPPRDIVER"
     gene            complement(4926..5531)
                     /locus_tag="VFQ35_04370"
     CDS             complement(4926..5531)
                     /locus_tag="VFQ35_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9929894.1"
                     /translation="MSDPETRPSDGDRALLDAADRAMSRYAEGDARAFETVFSAIGTR
                     IYKFLRRLSGSDELARDLLQETLLRMHQARGAFRSGSAVLPWAYTIARNVFLDSARAR
                     RRRPRGVPLEEPGNELPVPADAESSLLARQSAAVVERALAQMTEARREAFVLLRFEGL
                     SVATAAEILGVSENAVKLRAFQAYEILRDELARLEAGRRAR"
     gene            complement(5579..>6973)
                     /locus_tag="VFQ35_04375"
     CDS             complement(5579..>6973)
                     /locus_tag="VFQ35_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HET9929895.1"
                     /translation="LTHPPKRIETVRFRQEILRELAERPELRSAVEKLWLQIGEVLSR
                     LEVAERGKRYDAIARRIDILRDVKYAVDLLESGFKTAKSPLSRIRELGVGVRESKGYK
                     DLCDLLDHEGHLATVELRVRLGQDGRIRSFDIVRASENTKNPFYVAPLRRFLGRLRWL
                     IGGYKVREAEIVGRLANQVFDGMQDVVVALLELGLQLEFYLAALALRDRAAAAGLDMC
                     LPELVDAGREPDTKDELVALFNPLLLLETRAPRPSNLTFAPGAIAIITGPNSGGKTRL
                     LEAVGLAQLLAQAGMFVPARRARLRFREGLFVSLAQEVTADAQEGRLGTELLRIRRMF
                     ERLDFNSLVILDELCSGTNPSEGEGIFELVVALLAELEPQAFITTHFLEFAARLARER
                     PLPGLEFMQVELDENKDPTYGFVPGVAPTSLAGKTAERLGVTREALEALVREKKREKK
                     TSLPPPAEPAVLRE"
BASE COUNT         1162 a         2346 c         2305 g         1160 t
ORIGIN      
        1 agcgagcaca cattcggagc cgcgccgcac cgcgacgtaa atgtctccgc tcgcggagtc
       61 gtaggtgagc atcgaaacgt cgtcgccctc gaatgcgacg tcgcccacgg cccaaccaga
      121 gcccgtgcgc tggaccgtga agagcccctt gcgggttgca accaagagtc gttcgatcga
      181 gccgcccatg cgccggaccc ctcacccgaa tctaggtacg gcagaccgcc ggcctgagct
      241 tcgggcctcg agtctacagc cgttccgcca gcgccggcga accttgcgtg cagtcgcaac
      301 cctacgaaat gagctacgtt ctcgccgggc gaatcaagtt tcgtggcagc gcgtaggaat
      361 tccatccctt cggacgtcca tgcgacggac gaggactcgc ttccggggtc ggaagatctc
      421 gggcgcctgg cgctgcgggc cgctgcgggc gacgcgcgcg cgttcgaact cctggtcgag
      481 caaacgagcg acaccttggt tcggttggga gcacggctca tgggcagcct cccggatgga
      541 gaagacgtag tgcaagaagc gtatgtgaaa gcataccgag ggttgatcga agggcgcttc
      601 gacgggcgag cacaggttcg aacttggttg ggtcgcatcg tggtcaacac cgccatcgac
      661 gcactccgag tccgtaaacg tgctccgtct ccccgagacg aagcgtacga agccgtcgac
      721 gcgagcgcgt ccgccgaaac gcttgtcgct ctggccgagc tcgatgattg gctctccgcg
      781 cttccacccg aacagcgagc ggcggtcgtc ttgaagagca tcgaaggaca tacctccgcc
      841 gaggtcgcgg acttgctggg gtgcagcgag ggcgcggtcg aacagaagtt ggtgagggcc
      901 cgcgcggcgc tccgccaaag gatgaagtct tgacgccgga agacgtcgaa gcgcgcctcg
      961 cccgactcgc gcgtcaaacc agcgctatca gcgctcgtgc tggcttctcg cggggggtcg
     1021 tcgcgcgcgt cggggcgcag gcggcgcgca gcttcgagca gcagctcgct cgttccgcgc
     1081 ggcccgtggt cgtcgcggcg gcgctcctgg cgctcgcggt gctcgcccta tcgctctcga
     1141 gtcaacaatc gctcgatcgt tcgctcgcga cggaggtcgg agcgccagga gccgaatggt
     1201 gatcccgact cgtctgcgtg tatacggcgc tttcgcagcc gtgttcttgc tcggcgcggc
     1261 cgcgggcgct tcggcggcgc acgcctacgt cacgcgcagc ttgctcggcg cagaagacgc
     1321 aggtgagctg cgcgatcgtc gccgcctcga ggcgttgcgc cgggagctcg ggctcgaccg
     1381 cgagcaatcc gaccatatct ggactattct ctcgcgccgc cgcccaggcc accgagcctt
     1441 ggtgcgcgcg gcgttcgagc gctgcggaaa cgagctccgc gacgagcgtg agaagctcga
     1501 cacggagata cgcaaggagc tgaaaccgag ccagcagcag cgattcgacg aactcgtacg
     1561 caccgcgcac gagcgcttcc cgttgggccg cgagccgcgc taggggtgag ctccgccggg
     1621 cagcagcaat cgtttgcgcc aaatgacttg ctgcgagcgg ccgacgtggg tacgcgcgcg
     1681 gggttgcagc accactctcg cctccgcgtt ccgaaatggc tcgcgctcat cgcgctgtca
     1741 ttcgcgccgc ggctcgcgca cgcggatccg acgcccgagg tcgtggtcga aggcgaacgc
     1801 gctcagccgg aagcgccgaa ccgcgcgcgc gaagtcgccg gctcggtcgt ccgcggcgaa
     1861 cgattgaggg ctcccggagc cggcacgaaa gaggtgctcc gcagtgaacc cggcgtgcag
     1921 ctcgtcgagc tcggcgggct cggggccccc gcgacggcat cgctccgagg agcgaccgca
     1981 acgcagaccc ccatttatct cgccggcgtg cgtctgaacg acgaagtcgg cggcaccgcc
     2041 aatttgtcgg acgtccccgc cttcatgctc gaccgaatcg aggtgtaccg gagccatgca
     2101 ccgctcgcgg gcgaccagca aggcatcggc ggagcaatct acctcgagcc acgtcgcgtg
     2161 gtgccgaacg aacgtcgcgc agcgctgggc ggcatggtgg gcagctacgg cagtcgctcc
     2221 ctttctgctt tcggcggagt ctctggtgcg gagagcaatg tcgctgccgg cgtagagttc
     2281 gccgccaccg cgaacgacta cgccttctac agcggaaacg ggacgatatt cacgcccggg
     2341 gacgaccgtt ccgcgcggtt acccaacgcg gacgtctcga gcaccagcgt ttgggtcgcg
     2401 gccgcgcacg acacgccgaa ggctcggctt cggttgttct ttcacaccgc caatcgggaa
     2461 cagggagcgc cccgctttgc gctcagcccc acgtatcacc cccgcgtacg cctttcgcga
     2521 cagctgttgg cgttgacggc gacggtgccc ctcgactcac tcaacggcag tgtagagctc
     2581 tcgacttcgg gcatttcatc gacgacgcgc atcgacgatc cggggaacga cctcacgttg
     2641 gcgcgtcccc acgtcgcgac tccgggcgag cgcgtcgaac agagcgttct cgctcgcgaa
     2701 gcaacgcgag gggggttccg attcgtcgaa cagctcaccg tcagcgaaga acgactgcgc
     2761 cgcttcgaag gctccgcttc ggcggatgcc gagcaggtcg cagcggaaag gcggcgagct
     2821 cggctcagcg tgggtgccga agctcccatc gccacaggtg gtcgcgtatt cgtgaacggc
     2881 gtcgtcggct gccttggcac gaccgcgctc gggcgcccga gctgtgactg ggaaccagtg
     2941 accggacggc ttggcgccgc atatcgcgtc gggaacttcg agctctcggc aaacctcggt
     3001 tcgtacacgc ggccgccaac gctgagcgaa ctctacggca cttcgctcct cgtgcgcggc
     3061 aacgacgagc tctcgcccga gcatggaacg acgctcgaag cgttgattcg ctaccagctc
     3121 gtccgagaag gccgcaggct cttatttttc gacgtgtctg cgttcggccg caaaagccga
     3181 gatctggtga catttcttcg tacgtcccaa aactacttcg tgccggtgaa ccgcgccaaa
     3241 acgcgcgttc tcggcagtga gttcatcgtc ggcgcgacgc cgctcgcggg gctcgaggtg
     3301 agcggcaacg caagcttgtt ggacccgcgt gacgagagcc ccgatcgtgc gccggcgaac
     3361 gacatgctcc cgttcttgag ccggctcacc gtcggagcgg ccgtgagtgg ccggcacgcc
     3421 ctcggtgggc ccaagctcgg cacgctggcg ctcacgttgc gagcaactta ccagtcgagc
     3481 cgctacgccg atttcgccgg gcagggcgtc atcccagagc aagctagcgt cgacgtcgag
     3541 gcagggctac tcgaagtagc tcgcttgctc accacgcggc ttcgcgtctc caatttgttc
     3601 gacgcgcggc gcttcgacgt cgtagggttc ccgctgccgg gacgaagctt gtttctttcg
     3661 ctggagctcg gattgtgaag aagattttgc tgcccctttg cgcggcggtc gcgtgcaacg
     3721 tcgaaaatac gacgccagac acgggaggcg tcggggtcgc cggcgccggc ggctgctcgc
     3781 ctgggatcag cgtcgtgctc tcggattacg cgtccacgca agtcgcgttg agcacactcg
     3841 aagggcagac actttcgcgc tccttcatct cgaccggttc ggcgaagacc gatggcctgt
     3901 cgttcgcgct ctcgggcgac gtcgtactgc cgagttcgcg cccggcttcg ggcagggtcg
     3961 ttctggtcga ccgcttcggg acgaacgtgc tcacctggct cgatccggcg acggcgcgtg
     4021 tgctcggaca gctcgcggtg ggtactggct tcgactcgaa tccgagcgac tacctcgagc
     4081 tcgacgacca attcgccttc gtcagtcgct acgggcaaaa tcccgaaccg ggtcgccaac
     4141 cgttcgacat cggtggcgat ctcctgctcg tcgactcgcg agcccaagag atcgtgggga
     4201 gcttgccgtt cgaagcgacc gaggatctac ctccccgccc ctcggggatg atgcgcctcg
     4261 gagacgagat cttggtctct ctagtacgaa acgcgcgcga tttttcgacg acgggcgaca
     4321 gcgaactcgc cggagtctcc gtctcgaccc gaaggctcga ctttcggatg cgctacgagg
     4381 ggctcaagca ctgcggccgc cccgcgctgt ctccgagcgg agacgttctc gccgtcgcct
     4441 gcacgggcgc gctcgacatt cgaggcgtcg tcgccgacgt gagccagagc gcgttgctgt
     4501 tgctcgacgc gcgcgagctg ccaccccggg agctccggcg cttctccgcc gctgagctcg
     4561 gtaaccaacc catccagagc tcagccactt ttgcacgcga cgcgctcttg ctcttgaaga
     4621 cccaaacctc caccgtcgga acggagaaca atcgctggct cgcgctcgat ctcgagagcg
     4681 gcaaggtgac caccctgctc gaagcgagtc cggacgcgca aggcaaaggc cgcggcgtgc
     4741 tgtacggagg catgagctgc tctccgggct gcagcgaggt ctgcttgctc gccgacgcgg
     4801 accggggcgc gctccagcgg gtgcgcttcg acccgctcgg agcgcccgag cttctgccgc
     4861 cgatcgtggt cgacgcgaag tcggggcttc cgccgcgcga catcgtcgag cgctgaggtc
     4921 gtcggctagc gagcgcggcg ccccgcctcg agccgtgcga gctcgtcgcg caggatttcg
     4981 tacgcctgaa aagcgcgcag ttttaccgcg ttttcgctca ccccgaggat ctcggccgcg
     5041 gtggctacgc tgagcccttc gaagcggagc agcacgaagg cttcgcgccg agcctcggtc
     5101 atctgcgcga gcgcgcgctc gacgaccgct gcgctctgtc gggcgagcag gctgctctcg
     5161 gcgtccgccg gaacgggcag ctcgttaccg ggctcttcga gcggaacgcc gcgcggacga
     5221 cggcgccgag cccgcgcgga gtcgaggaac acgttgcgcg cgatcgtgta agcccagggc
     5281 aacaccgccg agccgctgcg aaaggcaccg cgcgcctggt gcatgcgcaa gagcgtctct
     5341 tgcaggaggt ctcgcgcgag ctcgtccgag ccggacaatc ggcgcaaaaa cttgtagatg
     5401 cgcgtgccta tcgccgaaaa gacggtctcg aaagcacgtg cgtcgccctc cgcgtagcgc
     5461 gacatggctc gatcggcagc gtcgagtagg gctcgatcgc cgtcgctcgg gcgagtctcc
     5521 gggtccgaca cgggtcgcag cgtagagcga gcgcgcgccg cctcaaagcg tgaaggcgct
     5581 attcgcgcag cactgccggc tcggcgggag gcggaagtga agtctttttc tctcgctttt
     5641 tctctcgcac gagggcctcc agcgcttcgc gcgtcacccc gagccgctcg gctgtcttgc
     5701 cggccagcga agtcggcgcg acaccgggga cgaagccgta cgtcggatcc ttgttctcgt
     5761 cgagctcgac ctgcatgaac tcgaggccgg gcagaggccg ctcgcgagct agacgagccg
     5821 cgaactcgag gaagtgcgtc gtgatgaagg cttgcggctc gagctcggcc aaaagcgcga
     5881 ccacgagctc gaagatgcct tcgccttcgg acgggttcgt gcccgagcag agctcgtcca
     5941 ggatgacgag gctgttgaag tcgagccgct cgaacatgcg ccgaattcgc aagagctccg
     6001 ttccgaggcg gccctcctgc gcgtcggcgg tgacctcttg cgcgagcgaa acgaacaacc
     6061 cttctcgaaa gcggagccgc gcgcgacgcg ccggcacgaa catgcccgcc tgcgcgagca
     6121 gctgcgcgag cccgaccgct tcgagcaagc gcgtcttgcc gcccgagttc ggccccgtga
     6181 tgatcgcgat cgcacccggc gcgaaggtca gattcgaagg tcggggcgcg cgcgtctcca
     6241 agagcaacag cggattgaag agcgcgacga gctcgtcctt ggtgtccggc tcgcgaccgg
     6301 catcgacgag ctcgggcaag cacatgtcga ggccagccgc tgcggcgcga tctcggagcg
     6361 cgagcgcggc gaggtaaaac tcgagctgca acccgagctc cagcagcgca acgacgacgt
     6421 cttgcatgcc atcgaacacc tggttcgcga gccgcccgac aatctccgcc tcgcgcactt
     6481 tgtagccgcc gatgagccag cgcagacgcc ccaagaaccg acgcagcgga gccacgtaga
     6541 agggattttt cgtgttctcc gacgcgcgta cgatatcgaa gcttcgaatg cgcccgtctt
     6601 gcccgaggcg cacgcggagc tcgaccgtcg cgaggtgccc ctcgtggtcg agcaggtcgc
     6661 agaggtcctt gtagcccttg ctctcgcgga cgccgacgcc gagctcgcga atgcgcgaca
     6721 acggtgactt cgccgtcttg aatccgctct cgagcaagtc gaccgcgtat tttacgtcgc
     6781 gcaaaatgtc gatgcgccga gcgatggcgt cgtagcgctt accgcgctca gccacctcga
     6841 ggcggctcag cacttcccct atttgcagcc aaagcttctc caccgcgctg cgaagctccg
     6901 gtcgctcggc gagctcgcgc aaaatctcct gacggaagcg aaccgtttcg attcgcttgg
     6961 gcgggtgcgt gag
//
LOCUS       DASTKS010000209         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_254721, whole genome shotgun sequence.
ACCESSION   DASTKS010000209 DASTKS010000000
VERSION     DASTKS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254721"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFQ35_04380"
     CDS             <1..463
                     /locus_tag="VFQ35_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9929896.1"
                     /translation="SHGKVRAAAALLLAHFGDTSGEAELLRVLAGDVPDVGPDDEQAA
                     IEAAARLGIRAAIPLLERRAFSWFGRDPQGYHARIALARLGDARARAAILRGLDAWTF
                     GARSLSVAAAGKAGLVEARRRLETFLSRPDRADPAVVREALSLLDAAESAR"
     gene            460..1569
                     /locus_tag="VFQ35_04385"
     CDS             460..1569
                     /locus_tag="VFQ35_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9929897.1"
                     /translation="MNAPGTTASLRRALDDEGRLLDGVEVPDMARVKALYMHMRRVRR
                     LDERMQALQRQGRIGFYGSCTGQEAAPVAAALCVSPQDWVFPALRESSLALVRGLSLE
                     RYLAQLFGNGADTLKGRQMPSHHASRAIHHVSWSSAIGTQLPHAVGAAMAAKRLRRDA
                     VMVAFLGDGATSHPDFHAALNFAGVFAAPVLFICQNNQYAISLPAARQTASATFAQKA
                     SAYGVFADRVDGNDALVVLDGVERALTRARAGLGATLLECLTYRVGPHSSSDDPSRYR
                     EESEVRSWQARDPISRLAQYLQCVDPFALASLDADERAWDAEIDKKLGEIEGLPPPPR
                     ESLFHDVYAEVPWHLREQRDALLSSIPEAPTTRAL"
     gene            1603..>2735
                     /gene="lpdA"
                     /locus_tag="VFQ35_04390"
     CDS             1603..>2735
                     /gene="lpdA"
                     /locus_tag="VFQ35_04390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9929898.1"
                     /translation="MDAAYDAIVLGGGPGGYVCAIRLAQLGVKTACVEEEEYGGVCLN
                     WGCIPSKALIANAHYVEKSKHIGDHGVVVGNVSVDVSRMQDWKNGIVKKLTSGVRGLL
                     KSNGADVIEGRGKLVGPRKLEVTAKDGSKRTIEAKKGIVIATGSATIQVPGFEFDGER
                     IIGAREAVSLRKLPKRLVVIGGGVIGLELGMVYQSFGSELTVVELTPNLLPGIDPDCV
                     RLVERTITKRGGRILKSTRADGYERRGEEVVVKITTAEKSEELACDAVLVAVGMKPRS
                     KGIGLEEVGVAIDPRGFVTTNERCETNVPGVYAIGDVSGRPMLAHKASKEGEVCAEVI
                     AGHPAAKDWVTVPGIVFTEPEIATAGLTEQEAKEQGHQVKVGR"
BASE COUNT          453 a          879 c          949 g          454 t
ORIGIN      
        1 gtcgcacggc aaagtgcgcg cggccgcggc ccttttgctc gcgcacttcg gagacacgag
       61 cggtgaagcc gagctcctgc gggtgctcgc aggtgacgtc cccgacgtgg gccccgatga
      121 tgaacaggcc gcgatcgaag ccgccgcgcg gctcggtatt cgcgccgcga tccctctgct
      181 cgaacgccgg gcgttctctt ggtttgggcg agatccccag gggtatcatg cgcgcatcgc
      241 gctggctcgg ctcggggacg cacgggcgcg cgcggcgatt ttgcgaggcc tcgacgcttg
      301 gactttcggc gcgcggtcgc tcagcgtcgc cgccgctgga aaggcgggct tggtcgaggc
      361 gcggcgccgg ctcgagacgt ttttgtcgcg gcccgatcgg gccgatcctg cggtcgttcg
      421 cgaggcgctt tcgctcctcg acgcggcgga gtctgcgcga tgaatgcgcc cggaacgacc
      481 gcgagcttgc gccgcgcact cgacgacgag gggaggctcc tcgacggagt cgaagtgccg
      541 gacatggccc gcgtgaaggc cctctacatg cacatgcgac gcgtgcgccg gctcgatgag
      601 cgcatgcagg cgctgcagcg ccaagggcgc atcggctttt acggctcgtg cacgggacaa
      661 gaggcggcgc cagttgccgc tgcgctctgc gtgtcgccac aggactgggt ttttccggcg
      721 ctgcgcgaaa gctcgctcgc gttggtccgc ggcctctcgc tcgaacgtta cctggcccag
      781 ctgttcggca acggcgccga tacgctgaag ggccggcaga tgccgagcca ccacgcgagc
      841 cgagccattc atcacgtttc ctggtcgagc gcgatcggca ctcagctgcc ccacgccgtc
      901 ggtgccgcca tggccgccaa gcgcttgcgc cgcgacgcgg tgatggtcgc tttcctcggc
      961 gatggggcga ccagccaccc cgatttccac gcggccctga acttcgcggg cgtcttcgcg
     1021 gcgccggtcc tcttcatctg ccaaaacaac cagtacgcga tcagcttgcc cgcggcgcgt
     1081 cagaccgcgt cggcgacctt cgcccagaag gcaagcgcct acggcgtctt cgcggatcgc
     1141 gtggacggca acgatgctct cgtcgtcctc gatggtgtcg aacgagcgct cacacgcgcg
     1201 cgtgcgggtc tgggtgcgac cctgctcgaa tgcttgacgt accgcgtggg tccgcactct
     1261 tcgagtgacg acccttcgcg ctaccgcgag gagagcgagg tgcggtcctg gcaggcgcga
     1321 gatccaatct ctcgacttgc gcagtacctg cagtgcgtcg accctttcgc gctcgcgtcg
     1381 ctcgacgccg acgagcgcgc gtgggacgcc gaaatcgaca agaaactcgg cgagatcgag
     1441 gggctgccgc caccgccgcg ggagagcctg tttcacgacg tttacgccga ggtgccgtgg
     1501 cacttgcgcg aacagcggga cgcgctcctc tcgagcattc ccgaggctcc gacaacgcgc
     1561 gccttgtgaa gcggggaaga ctccgctatg cgagccatcg tcatggatgc agcgtacgat
     1621 gcgatcgttc taggcggtgg tcccggcggt tatgtttgcg ccattcggct ggctcagctc
     1681 ggcgtgaaga cggcctgtgt cgaggaagag gagtacggcg gggtttgcct caactgggga
     1741 tgcatcccct ccaaggcgct gatcgcgaac gcgcactacg tcgagaagtc gaagcacatc
     1801 ggtgaccacg gcgtcgtcgt cggcaacgtg tcggtcgacg tcagtcgcat gcaggactgg
     1861 aagaacggca tcgtcaagaa gctcacctcg ggcgtgcgcg gcctcttgaa gtcgaacggc
     1921 gcagacgtga tcgaggggcg cggcaagctc gtcggtccgc gcaagctcga agtgacggcg
     1981 aaggacggca gcaagcgaac gatcgaggcg aagaagggca tcgtcatcgc cactggttcg
     2041 gcgacgatcc aggtgccagg tttcgaattc gacggcgagc gcatcatcgg tgcgcgcgag
     2101 gcggtgagct tgcgcaagct cccgaagcgt ctcgtcgtga tcggcggcgg cgtcatcggc
     2161 ctcgagctcg gtatggtcta ccagagcttc ggcagtgagc tcacggtcgt ggagctcacc
     2221 ccgaacctac tccccggtat cgatcccgat tgcgtgcggc tcgtcgaacg cacgatcacg
     2281 aagcggggtg gtcgcatttt gaagagcact cgcgcggacg gctacgagcg ccggggcgaa
     2341 gaggtggtcg tcaaaatcac cactgccgaa aagagcgagg agctcgcctg cgacgcggtg
     2401 ctcgtcgcgg tcggcatgaa gccgcgttcg aagggcattg gcctcgaaga ggtgggcgtc
     2461 gccatcgatc cgcgtggttt cgtcaccacc aacgagcgct gcgaaacgaa cgtacccggc
     2521 gtttacgcga tcggcgacgt cagcggtcgc ccgatgctcg cccacaaggc gagcaaggaa
     2581 ggcgaagttt gcgccgaggt gatcgcggga caccccgccg caaaggattg ggtcacggtg
     2641 cccggtatcg tgttcaccga acccgagatc gcgaccgcgg ggctcaccga gcaagaggcg
     2701 aaagagcagg gccaccaggt caaggtcggt cgctt
//
LOCUS       DASTKS010000210         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_924771, whole genome shotgun sequence.
ACCESSION   DASTKS010000210 DASTKS010000000
VERSION     DASTKS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924771"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VFQ35_04395"
     CDS             <1..1780
                     /locus_tag="VFQ35_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET9929899.1"
                     /translation="GQMATEELAFLGALGKFGMGMVHGDGNTRQCMATAAVAYKQSFG
                     FDAPPFSYADFEESDVVVFVGANPCIAHPIMWERVCRNKNQPAIVVVDPRKTETAMAA
                     THHYAVRPKSDLSLLYAVANILIERGWLDERFIAEHTSGFDEFKAHVARFTLAGAEAS
                     TGISAERIEALAKLIYEGKRVSFWWTMGVNQSHEGVRTAQAIIALALMTGNIGRPGTG
                     ANSITGQCNAMGSRVFSNTTNLLGGREFTNEAHRNEVAELLKIDAARIPSEPSLAYDQ
                     IVEAILAGKIRGLWIVATNPAHSWINQAHLRDVFKRLDCLVVQDMYNDTETAQLADIM
                     LPAAGWGEKEGTFINSERRIGVIKRVCRAPGDALADFHIFQLVAEAWGVGEMFREWSS
                     PEAVFQIMKRLSRGRPCDITGIEDYAMLDREGGIQWPLPEGQKLEARERRLFADGRFF
                     TDDGRAKFYFADPRPLPEPTTAAYPLLLLTGRGSSSQWHTQTRTKRSEVLNKLSPKEL
                     YVEVSALDARELGVRPDEEVNVISQRGSVRARAFITNSVRPGEVFMPMHYDTMNRLTF
                     AAFDPYSRQPAYKACAVKITKANEVD"
     gene            2176..3936
                     /locus_tag="VFQ35_04400"
     CDS             2176..3936
                     /locus_tag="VFQ35_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET9929900.1"
                     /translation="MASPKPTSLSLLDRQKPESRRSARFDRRLWHRFGAIAKPYWTSE
                     ERWGARGLLVLLVLLLLGETEFGVLFNEQSGEFTSALAARDGARFWRSIRVFFALLVV
                     AVPINGFYYYVRDTLAIHWRRWLTFHLLGKYFRHRAYYELGADSAVDNPDQRIAEDIN
                     TFTQRSLSFLMIAISALLQLLAFSRVLWSISRVLVLFLVVYAFVGTLTTFLVFGKTLV
                     RLNFLQLRREADFRFSLVRVRENAESIAFYRGEGEEASQVKRRFREVFSNFKRLIAAT
                     RNLNLFQYGYGLITLVLPSVIIAPRVLAGELEVGHVVQAAGAFAAILAALTIFVDNFE
                     SLSKFAAGIERLDTFRRSLRACPAHHEPEQIQRVEGAALLLEHVTLQTPGAGRTLVKD
                     LSLGVVAGAGLVIVGPSGCGKSSLLRAIAGIWSSGAGVIVHPPERDMMFLPQHAYMVL
                     GDLRSQLLYPNRERPVRDEELLQVLSTVNLPDVVARFGGLDAELDFAKVLSLGEQQRL
                     AIARVLLTKPRYAILDEATSALDAENEEVLYRNLRESGTTLVSVSHRPSLLKYHEQVL
                     ELTGDGAFRLSSAKDYRFTA"
BASE COUNT          748 a         1296 c         1302 g          785 t
ORIGIN      
        1 cgggcagatg gcgaccgagg agcttgcgtt cttgggcgcg ctcgggaaat tcggcatggg
       61 gatggtgcac ggtgacggaa acacccgtca gtgcatggcg accgcggccg ttgcctacaa
      121 gcagtcgttt ggtttcgacg cgcccccttt cagctatgcc gacttcgaag agtcggacgt
      181 cgtcgtgttc gtcggcgcga acccgtgcat cgcgcacccg atcatgtggg agcgcgtgtg
      241 tcgcaacaag aaccagcccg cgatcgtcgt cgtcgatccg cgcaaaaccg agacggccat
      301 ggccgctacg catcactacg cggtgcgccc gaagagcgac ctctcgctcc tgtacgccgt
      361 cgccaacatc ctcatcgagc gcggatggct cgacgagcgc ttcatcgcgg agcacacgtc
      421 cggcttcgac gagttcaagg cccacgttgc gcgcttcacg ctcgccggcg ccgaggccag
      481 caccggaata tctgcggaac gaatcgaggc tctcgcgaag ctcatctacg aaggtaagcg
      541 cgtcagcttt tggtggacga tgggcgtcaa ccagagccac gagggcgttc gcactgccca
      601 agcgatcatc gccctcgcgc tgatgacggg caacatcgga cgacccggca ctggcgccaa
      661 ttcgatcacc ggacagtgca acgcgatggg ctcgcgcgtc ttcagcaata cgaccaacct
      721 gctcggcggc cgcgaattca cgaacgaagc gcaccgcaac gaggtcgccg agctcttgaa
      781 gatcgacgcg gctcgtatcc cgagtgagcc gagcctcgcc tacgatcaaa tcgtcgaggc
      841 gatcttggcc ggcaagatcc gcgggctctg gatcgtcgcg accaacccgg cgcattcttg
      901 gatcaaccag gcgcacctac gggacgtgtt caagcgcttg gactgcctcg tcgtgcagga
      961 catgtacaac gacacggaga cggcgcagct cgccgacatc atgctcccgg ccgctggctg
     1021 gggagagaag gagggcacct tcatcaactc cgagcgtcgc atcggagtga taaagcgcgt
     1081 gtgccgcgcg cccggcgacg cgctcgcgga ttttcacata ttccagctcg tggcggaggc
     1141 gtggggcgtc ggggagatgt ttcgcgagtg gtcgtcgccg gaagcggtgt ttcagatcat
     1201 gaagcggctg agccgcgggc gcccgtgcga catcactggg atcgaagact acgcgatgct
     1261 cgaccgcgag ggcggcatcc agtggcccct gcccgaaggg caaaagctcg aggcgcgcga
     1321 gcgtcgcctg ttcgctgacg gtcgcttttt tacggatgac ggccgagcga aattctactt
     1381 cgccgatccg cggcctctgc cggagccgac gacggcggcg tacccgctct tgttgctcac
     1441 cggacgcggg agctcgagtc agtggcacac gcagacgcgc acgaagcgct ccgaggtgct
     1501 gaacaagctc tcgcccaaag agctctacgt cgaagtttcg gcgctcgacg cgcgcgagct
     1561 cggcgtgcgt ccggacgaag aggtgaacgt aatttctcag cgtggcagtg tgcgcgctcg
     1621 cgcgttcatc acgaattcgg tgcgcccggg cgaagtgttc atgcccatgc actacgatac
     1681 gatgaaccgg ctcacgttcg ccgcgttcga tccctactca cgacagccgg catacaaagc
     1741 gtgcgccgtg aagatcacga aggcgaacga ggtcgattag cgggctaggg ggtgtcccaa
     1801 gttcctgccc gtcgcgagag ccaagaaacg cgagcgagaa gcgcgcccga ggagagagca
     1861 ccggaggaag gctgaagcct ttcggggagc ggagcgacga ggccgtgctt cgcagccgca
     1921 ggatcttggc tcgcagcagg gcggggaact tgggacaccc cctagtatca atccggaaaa
     1981 ttccagggaa tccaggcgat gaatcgccaa ttattgctcc gagaatcgtc gttggattgg
     2041 cgactgagaa ggtcgctccc gcggcgcaag agtcgcgtgg acatcggaga tacgcagtct
     2101 gtgcgccgtt cgtccatgga gttgctcgaa agctcgcgcc gttcgcgcgg cagacacttt
     2161 gcatgagacc atcgcgtggc ttcaccgaag ccgacttcgc tctcgctcct cgaccgccag
     2221 aaacccgagt cgcggcgctc cgctcgcttc gacagaagac tctggcatcg cttcggtgcg
     2281 atcgcgaagc cgtattggac ttccgaagag cgttggggag cccgtggcct gctcgtgctc
     2341 ctcgtcttgc ttctgctcgg agagacggag ttcggggttt tgttcaacga gcagagcggc
     2401 gagtttacct ctgcgctcgc ggctcgagac ggcgcccgct tttggcgatc cattcgtgtg
     2461 ttcttcgcgc tgctggtcgt cgcggtgccg atcaatggct tttattacta cgtgcgtgac
     2521 acgttggcga tccattggcg acgctggctc acgtttcact tgctcggcaa atactttcgc
     2581 catcgcgctt actacgagct cggggcagac agcgccgtcg ataaccccga ccagcgcatc
     2641 gccgaggaca tcaacacctt cactcaacgc tcgctgagtt ttctgatgat cgcgatcagc
     2701 gcgttgctcc agctcttggc cttcagtcgg gtgctgtggt cgatctcccg agttctagtg
     2761 ctgtttctgg tggtctacgc gttcgtcgga acgctcacga cttttctcgt tttcggcaag
     2821 acgctcgtgc gcctgaattt cctgcagctg cgacgagaag cggacttccg tttcagcttg
     2881 gtgcgggtga gggagaacgc cgaatcaatc gctttttacc gcggagaagg cgaggaggcg
     2941 tcccaggtga aaaggcgctt tcgtgaggtg ttctccaatt tcaaacgact aatcgccgcg
     3001 acccggaact tgaacctctt ccagtacggc tatggcctca tcacgctcgt gttgccgagc
     3061 gtcatcatcg cgccacgcgt gctcgcgggc gagctggagg tcgggcacgt cgttcaggcc
     3121 gccggagcgt tcgccgcgat cttggcggcg ctcaccatct tcgtcgacaa cttcgagtcg
     3181 ctcagcaagt ttgcggctgg catcgaacgt ctcgacacct ttcgaaggtc actcagggct
     3241 tgtccagctc accacgagcc cgagcaaatc cagcgagtcg agggcgccgc gctgttgctc
     3301 gaacacgtca cgttgcagac acccggcgcg gggcgaacgc tcgtgaaaga tctctcgctc
     3361 ggcgtcgtcg cgggcgcagg gctcgtcatc gtcggcccga gcggctgcgg caagagctct
     3421 ctgcttcgcg cgatcgccgg aatctggagc tcgggggcgg gcgtgatcgt tcacccgccc
     3481 gagcgcgaca tgatgttctt gccgcagcac gcatacatgg tgctcggcga tcttcggagc
     3541 cagctcctct acccgaaccg ggagcggccc gtgcgcgacg aggagctcct gcaagtgctc
     3601 agcaccgtga atctgcctga tgtcgtcgcg cgctttggtg gcctcgacgc ggagctcgac
     3661 tttgcgaagg tcctctcgct cggagagcag cagcgcctgg ccatcgcgcg ggtcctcctc
     3721 acgaagcctc gctacgccat cctcgacgag gccacgagcg ccttggacgc ggagaacgag
     3781 gaggtacttt accgaaacct gcgagaaagc gggacgacgc tggtcagcgt tagccaccgt
     3841 ccgagcctcc tcaagtatca cgagcaggtt ctcgaactga cgggcgacgg agcgttcagg
     3901 ctttcgtcag cgaaagacta ccgcttcact gcttgaccat cgggcctacg tctccgactg
     3961 agccgtcgcg aacgtttcgc cggcgtgaac agctccaacc cgccgctcgc caatgctcgt
     4021 tcgaatgcct ctcgagggtg gcgggcccca cgagacagtg caagttcaca ttgtaagtcc
     4081 ctgtcctgaa gggtagtgcg ttcctcgccc atgagtctct tgccgttgtt c
//
LOCUS       DASTKS010000211         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1594984, whole genome shotgun sequence.
ACCESSION   DASTKS010000211 DASTKS010000000
VERSION     DASTKS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594984"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFQ35_04405"
     CDS             complement(<1..568)
                     /locus_tag="VFQ35_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929901.1"
                     /translation="MAVADPVAQAMSALHRIESSGGAAMAEAAPTALNFVRQALAQLQ
                     SAQVSHPAVDRGIEAIAGSLGLVHQLAQSVASATPAPAGAPAVSPYAGTMMDPAAAMA
                     AAQPPATPGYVKPAATVASPPPVQARPAPPAAGIAYASPPAAAPAPPVRPAAPMPGGM
                     QRFEAALGAHSPTNFYKGLSGNDVIESGG"
     gene            complement(695..1633)
                     /locus_tag="VFQ35_04410"
     CDS             complement(695..1633)
                     /locus_tag="VFQ35_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET9929902.1"
                     /translation="MPLRIAYFGLPLGALLLDRDGHELSFCTVPPLDAPGLRRLKRRF
                     EGRVLEAFELGDALEDEVAMRLAEEPPDILVSWFWTRKLPLEWLRVPRLGAIGVHPSL
                     LPRHRGPNPYFWAIDGGDVETGVSVHRLEAEYDTGAILEQERIVIGERNAWQLARALD
                     RPSLRCLRRTVAAATRGLPVGIEQTPELATLAPEPQGELLRADFKWPTERILRRIRAL
                     SPVPGLALSIADVAFFVTRAHPSAEFTRALLPGEAEIARGRLVIRTGDAAIAVETGVL
                     VGEDDDSEEVSGAELAEHMAQAISLARGTSLKGDGG"
     gene            1744..2532
                     /gene="ftsE"
                     /locus_tag="VFQ35_04415"
     CDS             1744..2532
                     /gene="ftsE"
                     /locus_tag="VFQ35_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET9929903.1"
                     /translation="MDLSGKTRSFRPALRGQVRFDPLAANRPILVFEGVHKAYRADAP
                     VLRGLSLEIARGEFVFITGPSGAGKSTLLRLLYAAEAADSGRILFLGREIGRLTETSI
                     PFLRRNIGVVFQDFKLVGNWTVFDNVAIPLEVLGLPQRVIRQRVGEALERVGLGGRGS
                     DIARHLSGGEQQRVAIARAIVGEPALVLADEPTGNLDPQLAVDILGLLEDINATGVSV
                     IFATHDRTLLDVRPRRVVVLDEGKALDVRNGLDEESSLNGLQVA"
     gene            2552..3421
                     /locus_tag="VFQ35_04420"
     CDS             2552..3421
                     /locus_tag="VFQ35_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET9929904.1"
                     /translation="MTPLERAWRGARSDIRLYALSVFSVAVAFVCLAAAVLVVVNVHG
                     VRARWADSGRASVFLRAAAAPDQVDALERALKKTPGIAVVRRVTSDQARRELSGGSTD
                     PVFDALPSDVFPASLELELEPGLLPTRLEKLKTELSTLPVVDTIQTYEAWSDRLGALL
                     SGGVTASLLLAAVVLGAVVSVVSSTIRLSLQRRHIEVEVLKLVGATDAYVRRPFVVEG
                     AAQGGIGAGLAVLLLGALFLTVRSQVDPSLFTLLGIEPVFLPLPLAFGLIVLGCALGA
                     LSALFSLRKLLVV"
     gene            3418..4374
                     /locus_tag="VFQ35_04425"
     CDS             3418..4374
                     /locus_tag="VFQ35_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9929905.1"
                     /translation="MKAKLAAAALTSLFCSSLALADATRGSDREVPHAAADIEKLVTT
                     LEREQRDALARFDALGKEAEAAGARAAVRGRAYARLARAGFLPVGGGFRSFIDHATRL
                     ERLRRGLKADVTLQHSATVERAKLARRLEELKLRLVPLQAEHAALARAEDALLSAQDR
                     ERAFQRAFESSSNGHTAVYGAQGPSDPSEVSIGFASLRGRLPFPIAGRSEIKSARRSG
                     ADGPALEMRAPLGTVVRAVYPGRVAFADTYAEYGKTVIVDHGNRHYTVSANLGSIDVA
                     VGDEVVLNTRVGTVGDSGHGALLYFEIRVGTETVDPAEWFGI"
     gene            4826..5245
                     /locus_tag="VFQ35_04430"
     CDS             4826..5245
                     /locus_tag="VFQ35_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929906.1"
                     /translation="MPAGYHVEHRAARGLIGTGIATAAIGYVAGLGVAMSHDFDGSLG
                     WMAVPVVGAWPAVAGSKVTCTAQDVPAAKQCLSDAYNQATTIAIVAVDGMVQATGVVL
                     LVAGLLSGHSELVRDDVQVSARQRPEGGFDLSVRGSF"
     gene            5316..5798
                     /locus_tag="VFQ35_04435"
     CDS             5316..5798
                     /locus_tag="VFQ35_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929907.1"
                     /translation="MTTRIDSNGPAPLVATLPSAARTTSTPSRPFRQFVAASANVVIQ
                     GAEQAVTRLPGGSLLAASVRPAGAPPSLASQLPEGASFAPGLAPSAIGGSSAPISAGV
                     AGQQTGGVEGVLAQGVDQNMYFLELQERISQESRTYSALSNVLKARHETLKNAIGNIR
                     "
     gene            5878..6240
                     /locus_tag="VFQ35_04440"
     CDS             5878..6240
                     /locus_tag="VFQ35_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929908.1"
                     /translation="MSIDGIGRPPGGPGGAGGVGSLGGAAKPTPGGESFQVQRSAAAP
                     SASSDPLSRLQSGEITVDQYLDARVDEAVAPLVSKLPAEALEHIRSSLREQLESDPTL
                     VELVRRATSAAPPGSTET"
     gene            6245..>7096
                     /locus_tag="VFQ35_04445"
     CDS             6245..>7096
                     /locus_tag="VFQ35_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929909.1"
                     /translation="MPGSRGYRELTLEQAKELEGVWVTAGLSDAPSVPPTPLGWDLAL
                     DDVEILGDALERRGASLPRPGRRFASVAGYAYHALVPLVRAARELVPTDAVALSHAVA
                     CEALPAARSTLPAQRRPRQSLALLSARISRGLTRLDARVLAHERDASQHYRWLVEMDL
                     GILPDDALKTTLAECLAVYRSSRSLELEATLDLVGCHAALLALARNLPDSEREAFACA
                     ALVPDALDLATATPSLALFSSSRRAQSQSAALPSSADFLGGFGERSSRESEPYAPRWS
                     EVPWLLDR"
BASE COUNT         1058 a         2437 c         2418 g         1183 t
ORIGIN      
        1 tgccgccgga ctcgatgacg tcgttgccgc tcagaccctt gtaaaagttc gtcgggctgt
       61 gggcgccgag cgcggcctcg aagcgctgca tgccgcccgg catgggggcc gcgggacgaa
      121 ccggcggagc gggtgcggct gcgggcggac tcgcgtaagc gatgccagcc gcgggcggcg
      181 cgggccgagc ttgaaccgga ggcgggctcg ccacggtcgc cgccggcttc acgtatccgg
      241 gggtcgccgg gggttgggcc gccgccatcg ccgcagcggg atccatcatg gtgcccgcgt
      301 acggcgaaac cgcgggagca cctgcgggag cgggcgttgc cgaagcgacc gattgcgcga
      361 gctgatgcac caagccgagc gaccctgcga tcgcttcgat gcctcgatcg acggccgggt
      421 gcgagacctg cgcgctctgc agttgcgcga gcgcttgccg tacgaagttc agcgcggtcg
      481 gcgcggcctc ggccatcgcc gctccgccgc tcgactcgat gcgatgcagc gcgctcatcg
      541 cttgcgccac cggatcggcg accgccatga gctgcggagg taggccgggg ctttgaagcg
      601 cagctagccc tttcgcaaga ttttcacgtg cagaccgagc ggcgccgagc gcgtccattc
      661 tccatgacct ccaaaagatt gtcgctcgag tctatcaccc gccgtcacct ttgagggaag
      721 ttccccgcgc gagcgaaata gcctgcgcca tgtgctcggc gagctccgct ccgcttacct
      781 cttcggagtc gtcgtcttcc ccgacgagca cgcccgtttc gaccgcgatc gccgcgtctc
      841 ctgtcctgat gaccaagcgc ccgcgggcga tctccgcctc acctggcaaa agtgcgcggg
      901 taaactctgc gctcgggtgg gcacgcgtca cgaagaaggc cacatctgct atcgacagcg
      961 cgagcccggg cacgggggag agcgcgcgga ttcgccgcaa gatgcgctcg gtcggccact
     1021 tgaagtcggc gcgcaggagc tcgccctgcg gctcgggggc gagcgtcgca agctccgggg
     1081 tctgctcgat gccgacaggc aggccacgcg tggctgcggc gacggtgcgc cgcaaacagc
     1141 gcaaactcgg ccgatccaga gcgcgggcga gctgccaggc gttgcgctca ccgatgacga
     1201 tgcgctcctg ctccaggatc gcgcccgtgt cgtactcggc ctcgagtcgg tgaacgctga
     1261 cgccggtctc gacgtcgccg ccatcgatgg cccaaaaata cggattcggg ccgcggtgtc
     1321 gcggcaacag cgacggatgc actccaatcg cgccgaggcg gggcacccga agccactcga
     1381 gcggcagctt gcgagtccaa aaccagctca ccaaaatatc cggcggctct tcggcaagtc
     1441 gcatcgccac ctcgtcttcg agcgcgtcgc cgagctcgaa cgcctcgagg acgcgcccct
     1501 cgaaccggcg cttgagtcga cgcaaccccg gcgcgtccaa cggcggcacc gtgcagaacg
     1561 agagctcgtg cccatctcga tcgaggagca gcgcgccgag cggtaaccca aagtacgcga
     1621 tccgaagcgg caccggcctc atcttagagc tccgcgccgc ccgggcgcca tcggccttcg
     1681 ggcctgtcga cgtccggccg aaaacttgtt cagggcgaat gctccctgtt acctgggagc
     1741 cacatggact tgagcggcaa gaccaggagc tttcgtcccg ccttgcgggg tcaggtccgc
     1801 ttcgacccgc tcgccgccaa tcggccgatc ttggttttcg agggcgttca caaggcttac
     1861 cgcgccgacg cgccggtgct gcgcggccta tcgctcgaga tcgcgcgggg cgagttcgtg
     1921 ttcatcaccg ggccttcggg cgcgggcaag agcacgttgc tccggttgct ctacgccgcc
     1981 gaggctgccg acagtggtcg aatcctattt ttggggcgcg aaatagggcg cctcacggaa
     2041 acgtcgatcc cgtttttgcg ccgcaacatc ggcgtcgtgt tccaagactt caagctcgtc
     2101 ggcaattgga cggtgttcga caacgtcgcg atcccgctcg aggtgttggg gctcccgcag
     2161 cgagtgattc ggcagcgcgt cggcgaggcg ctcgagcgcg tcggcttggg cggccgaggc
     2221 agcgacatcg cgcgacactt gtcgggcggt gagcagcagc gcgtcgcgat cgcgcgagcc
     2281 atcgtcgggg agccggcgct cgtgttggcg gatgagccca cggggaacct ggatcctcaa
     2341 ctcgcggtag atattttggg tttgctcgag gacatcaacg ccactggcgt ctcggtgatc
     2401 ttcgcaacgc acgatcgcac gttgctcgac gtgcggccgc gtcgcgtggt cgtgctcgac
     2461 gaagggaagg ccctcgacgt gcgcaatggt ctcgacgagg agtcgtcgct caacggcttg
     2521 caggtagctt gatgaagcgg agcgagaatt catgacgcct ttggagcggg cgtggcgcgg
     2581 cgcgcgcagt gacattcgcc tgtacgcgct cagcgtgttc tcagtcgcgg tcgccttcgt
     2641 gtgcctggcc gcggcggtgc tcgtcgtggt caacgttcac ggcgttcgcg cgcgctgggc
     2701 ggacagcggc cgcgcttcgg tatttctgag agcggcggcg gcaccggacc aggtcgacgc
     2761 gctcgaacgc gccttgaaga aaacgccggg aatcgccgtg gttcgccgcg tgacgagcga
     2821 ccaggctcgc cgggaactgt cgggcggctc gacggatccg gtcttcgacg cgctcccttc
     2881 ggacgtgttc ccggcgtcgc tcgagctcga actcgagccc ggcctcttac cgacccgtct
     2941 cgaaaagttg aagacggagc tttccactct gcccgtcgtc gacaccattc agacgtacga
     3001 agcctggagc gaccgcctcg gcgcgctgct gagcgggggc gtcacggctt cgttgctgct
     3061 cgcggccgtg gtgctcggtg ccgtcgtcag cgtcgtctcc tcgacgattc gcttgagcct
     3121 ccagcggcgt cacatcgagg tcgaggtgct gaagctcgtc ggcgcgaccg acgcctacgt
     3181 gcgacgcccg ttcgtcgtcg aaggcgccgc gcaaggcggc atcggcgcgg gcctcgcggt
     3241 cctgctcttg ggcgcgctgt tcctcacggt gcgcagccag gtcgacccgt cgctgttcac
     3301 tctgctcggc atcgaaccgg tgtttttgcc gcttccgctc gcgttcgggc tcatcgtgct
     3361 cggctgtgcg ctcggagcgc tatcggcgct atttagcctg aggaagctct tggtcgtatg
     3421 aaagcgaagc ttgctgcggc cgcgctcacg tcgctcttct gctcgagcct cgcgctcgcg
     3481 gacgcgaccc gtggctccga tcgagaggtg ccgcatgccg ccgccgacat cgagaagctc
     3541 gtgaccacgc tcgagcgaga gcagcgtgac gcactcgcgc gcttcgacgc gctcggcaag
     3601 gaagcagagg cagctggagc gcgcgccgcg gtccgcggac gtgcctacgc gcgcctcgca
     3661 cgcgctggat ttctccccgt gggcggaggg ttccggtcgt tcatcgatca cgcgacgcgc
     3721 ctcgagcgct tgcggcgcgg gctcaaggcg gacgtaactc tgcagcacag cgcgaccgtc
     3781 gagcgcgcga agcttgcgcg gcgcctcgaa gagctgaagc ttcggctcgt gccgctccaa
     3841 gccgagcacg ccgcgctcgc gcgcgccgag gacgcgctgc tgtcggcgca agaccgagag
     3901 agagcgtttc aacgcgcgtt cgagtcctcc tcgaacgggc acaccgccgt gtacggcgcg
     3961 caaggtccga gcgacccgag cgaagtctcc atcggctttg cctccctacg cggacggctg
     4021 ccgttcccca tcgcgggccg ctccgaaatc aagagcgcac gccgcagcgg cgccgacggc
     4081 cccgcgctcg agatgcgcgc cccgctcggt acggtagtgc gcgcggtgta ccccggacgg
     4141 gttgcctttg cggatactta cgccgagtac ggaaagaccg tcatcgtgga tcacggtaac
     4201 cggcactaca cggtcagcgc gaatctcggc tcaatcgacg tcgcggtggg cgacgaggtc
     4261 gttttgaaca cccgcgtcgg caccgtcggc gactcaggtc acggcgcgct cctctacttc
     4321 gagatccgcg tcggcaccga gaccgtcgat cccgcggagt ggttcggtat ctgacggctt
     4381 cccacacgcg aggggtcgga gtgcgtaacg ctcgcaagtg cttgcggtgc gtagctcagg
     4441 gcgctagcgt tttcttgtgt ctgggcggtg tctactcgag ccgtgtgctc ggttttgttt
     4501 tgggttttgc gtagcgctcg cgtggggagc cccggcagcg gcacaaagcg ctggttttcc
     4561 tatcgaccca ccgccgaacg ccgcgccctc cgcgccgcct ccggccccag cacctgcggc
     4621 acctgcgcca gcgcagccac gaccaagcgt cgtagctccc cccgcgcaac gcagcgcgcc
     4681 ggtcgcacca accacggcgc cgcccgcgcc tgtcgcgccc gtcgcaccag cggcaccctc
     4741 tctggcagaa gagccggcag cgcctccgcc gagcgtcgtt gcgttgggag cgccggcgat
     4801 ccttccgtac cgggatggct ttccggtgcc cgccgggtac cacgtcgaac atcgcgcagc
     4861 gcgagggctc atcggaaccg gcatcgcaac cgcagcgata ggatacgtgg ccgggctcgg
     4921 cgtggcgatg agccacgact tcgacggcag cctgggctgg atggccgtgc ccgtcgttgg
     4981 cgcgtggcca gcggttgcgg gcagcaaagt gacgtgcacg gcgcaggacg ttcccgccgc
     5041 gaagcaatgt ctcagcgacg cctacaacca ggccacgacg atcgccatcg ttgccgtcga
     5101 cgggatggtg caagcgaccg gcgtggtgtt gctcgtcgct gggctcctga gcggtcactc
     5161 cgagttggtt cgcgacgacg tccaggtgag cgcccgccag cggcccgagg gagggttcga
     5221 tctcagcgtg cgcggcagct tctgatcgag accgcttttt tcgagcaacc ggctccgggc
     5281 cgtgaccgaa ggagtgggtg cctaaggagg catccatgac tactcgcatc gattcgaacg
     5341 gtccggctcc cctcgtcgca accctcccga gcgcggcgcg caccacttcc accccgtcgc
     5401 gcccttttcg ccaattcgtc gccgccagcg cgaacgtcgt gatccaaggc gcggagcaag
     5461 cggtcacgcg cttgccagga gggtcgctgc tcgctgcatc cgtgcgcccg gccggcgccc
     5521 caccgagcct cgcgtctcaa cttcccgagg gagcgagctt tgctccaggg ctcgcgccat
     5581 ccgcaatcgg cggcagctcg gcgcccatca gcgcgggggt ggcaggccag caaaccggcg
     5641 gcgtcgaagg cgtgctggcg cagggagtgg accaaaacat gtactttctc gagctgcagg
     5701 agcgcatcag ccaggaaagc cgaacctatt cggcgctttc taatgtgctg aaggcacgcc
     5761 acgagacttt gaagaacgcg atcggcaaca tccgctgagg gctccttttg caccacccct
     5821 tcgaaggatt gcggccattc tccgccgaca tctggcatca ttgaggcagg aagagcgatg
     5881 agcatcgatg gcatcggtcg ccccccgggc gggcccggcg gcgccggcgg cgtagggtcg
     5941 ctcggcggcg cggcaaagcc gaccccaggc ggggaaagct ttcaagttca gcgcagcgcg
     6001 gccgcgccga gtgcctcgag cgatcccctg tcgcgcctcc agagcggcga aatcaccgtc
     6061 gatcagtacc tcgacgcgcg cgtcgacgag gcggtcgcgc cgctcgtgtc gaagctgccg
     6121 gcggaggcgc tggagcacat tcgcagctcg ttgcgcgagc aactcgagag tgatccgacg
     6181 ttggtcgagc tggttcgacg ggctacgtcg gcggcgccgc cgggctcgac cgagacctga
     6241 agggatgccc gggtcgcgcg gctatcgcga gctcaccctc gagcaagcga aggaactcga
     6301 aggagtgtgg gtgacggcgg ggctctccga cgcgcccagc gtaccaccca cgcctctcgg
     6361 ctgggacttg gcgctcgatg acgtggagat tctcggcgac gcgctcgagc gccgaggcgc
     6421 gtcgctgccg cgacccgggc ggcgcttcgc ctccgttgca ggatacgcgt atcacgcgct
     6481 agttcctctc gtgcgcgccg cccgcgagct cgtccctacc gacgcggtcg cgctctccca
     6541 cgccgtcgcc tgtgaggcgc tgcccgcggc gcgctcgacg ctcccggcgc aacgtcgtcc
     6601 ccgccagagt ctcgcgctgc tcagcgcgcg catcagtcgc gggctcaccc gactcgacgc
     6661 gcgcgtactg gcgcacgagc gcgatgcttc gcagcattac cgctggctcg tcgagatgga
     6721 tctcggcatc ttgccggacg acgcgttgaa gacgacgctc gcagagtgtc tcgcggtgta
     6781 tcgctcgtcg cgttcgctcg agctcgaggc gacgctcgat ttggtcgggt gtcacgcggc
     6841 cttgctggcg ctcgcgcgca accttccgga ctccgagcgc gaagcgttcg cgtgcgccgc
     6901 gctcgtgcca gacgcgctcg acttggccac ggcgacgccg tcgctcgccc tcttcagctc
     6961 gtctcgtcgc gcgcagagcc agagcgcggc gctcccgagc tcggcggatt ttttgggcgg
     7021 tttcggcgag cgatcctcgc gcgaaagtga gccctacgcg ccgcgctggt ccgaggtgcc
     7081 gtggctgctc gatcga
//
LOCUS       DASTKS010000212         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_701709, whole genome shotgun sequence.
ACCESSION   DASTKS010000212 DASTKS010000000
VERSION     DASTKS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701709"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFQ35_04450"
     CDS             complement(<1..1241)
                     /locus_tag="VFQ35_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET9929910.1"
                     /translation="MKNEPKPRATNTSTDSPDSGVVEAVPNSGLRTLEPSRGSDERGL
                     GSTKTESSATREQLTRVTESERASLLLELVARVTLELSRADGPRFDADAPWRQLGIYQ
                     QAARELCTRLSAEVAHPLGATLLFDYPTPRCLARHLSIQLLGESAAFEPPVPERSLLH
                     DDPIAIVGMGCRYPGGVRTPADLWTLVSEGRDAVSELPTNRGWDLERIYDPEAGKPGK
                     TYVRNGAFLHDADAFDAGFFGIGPREAKALDPQQRLLLETSWEALERAGIDPSSLRGT
                     RTGVYVGIAYQDYGPNWHEAPPELSGQLLMGSLTSAASGRVAYTLGLEGPALTIDTAC
                     SSSLAAIHLGCQALRAGECSLALAGGATVMATPGVFLEFASKRGLSPSGRCKSFSADA
                     DGTGWGEGAGVLVLERLSDARR"
     gene            complement(1288..2835)
                     /locus_tag="VFQ35_04455"
     CDS             complement(1288..2835)
                     /locus_tag="VFQ35_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929911.1"
                     /translation="MKRVYSGWAVLALVLAATACSGSDGGSPSGHDPSLPTSPNANAA
                     APSTPSAPAAPNTPTSEATPTAMNDGAVDVATNAPGAPASEPSTPAAPGPDNTAIPAA
                     PALENSILGTITDLGTGNPADGLGGVGTLAKAATVRVSQLVGGTLALLGETNVGANGT
                     YSLAIPVQAGTLVAEALDAVGNVLGAVVVDASNNAAKNVVAPPMTSETSLEANVVVNS
                     TSCAVAPAPLNSQVDLSLVQNVLSLIDTDLTKAITSAIAAGVDPAQIVEALVNAVTAA
                     VHAQTGVVSAVDITATGDPAHVRAQVAVRLAFSSTLANSLANVAQADKIVFAAIHSAA
                     QLEAKLTADAVKNIIAAAGATQELVNQATQIGDALIAAVAKATNIPALKQAQLDFVSD
                     LLGSVNGTANGGLIGNLLTQTTGTVNSLLGSVLGKVETLASTLDSNLTGNLPALAGSN
                     VCVDVNAAAKLDLNVQALTGSLTQFVGDIAALGPDLLKGGNGKLPVSALTDLLSTAQM
                     LLRGLLP"
     gene            3063..>3367
                     /locus_tag="VFQ35_04460"
     CDS             3063..>3367
                     /locus_tag="VFQ35_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9929912.1"
                     /translation="MRSPAEDATTRIRAEVRGLRVLSFDLVVVDGPSRGARVHIAGGV
                     ARVGSAPGNDLALSDATVSRVHCELRAQSDGVVVKDCGSTNGTFVEGVRLREGDVKPG
                     "
BASE COUNT          508 a         1060 c         1193 g          606 t
ORIGIN      
        1 cggcgagcgt ccgagagccg ctccaagacg agcacgcccg cgccttctcc ccagccggtc
       61 ccgtccgcgt ccgccgagaa ggatttgcag cgcccgctcg gcgaaagtcc acgcttggac
      121 gcaaactcga ggaacacgcc cggtgtggcc atcaccgtgg ctccgccggc aagcgccaac
      181 gagcactctc ctgcacgcaa cgcctggcaa ccgagatgga tcgcggccaa cgacgacgaa
      241 cacgcggtat cgatggtgag cgctgggcct tcgagaccca gcgtgtatgc gacgcggccc
      301 gaggcggcgc tcgtgaggct acccatcaag agctgtccgc tgagctccgg cggcgcctcg
      361 tgccagttcg gtccgtagtc ttggtaggcg atcccaacgt agacccccgt gcgagtgccg
      421 cgcagcgacg aaggatcgat cccggctctt tcgagcgcct cccacgaggt ctcgaggagc
      481 aaacgctgtt gcgggtcgag cgcctttgcc tcgcggggcc cgatgccgaa gaagcctgca
      541 tcgaaggcgt cggcgtcgtg caaaaaggct ccgtttcgca cgtaggtctt gccgggcttg
      601 cccgcctcgg gatcgtaaat gcgctccaaa tcccagccgc ggttcgtcgg gagctccgaa
      661 actgcgtccc ttccttcgct caccagtgtc cagagatccg cgggagtacg aacgccgccc
      721 gggtatcggc agcccatccc cacgatcgcg atcggatcgt cgtgtaagag ggagcgttcg
      781 ggtacggggg gttcgaacgc ggcgctttcg ccgagtaatt ggatcgagag gtggcgggcg
      841 aggcagcgcg gggtcgggta gtcgaagagc agggtcgcgc cgagcggatg ggcgacctcg
      901 gccgagaggc gcgtacacag ctcgcgcgct gcctgctggt agatgccgag ttgccgccaa
      961 ggcgcgtccg cgtcgaagcg cggcccatcc gctcggctga gctcgagcgt cacccgggcg
     1021 acgagctcga gcagcaagga cgctcgctcc gactccgtca ctcgggtgag ttgctcgcgc
     1081 gttgcgctcg attcggtctt cgtcgagccc aggccgcgct catcggaacc gcgggacggt
     1141 tccaaagtgc gcagcccaga attgggcaca gcttccacga cgcccgagtc gggcgaatca
     1201 gtcgaggtgt tcgtggctct gggcttgggc tcgttcttca tgcgactcct cgttcttccg
     1261 ttggctcttc gggggacacg cggggggtta cgggagcagg ccgcgcaaga gcatctgcgc
     1321 ggtggagagc aggtccgtga gcgcggagac gggcagtttg ccgttgccgc ccttcagcag
     1381 gtctggcccc agcgccgcga tgtcgccgac gaactgcgtg agcgaaccgg tgagggcttg
     1441 cacgttcagg tcgagcttgg cggcggcgtt cacgtcgacg caaacgttgg agccggcgag
     1501 ggcgggtagg ttccccgtga gattggagtc gagggtgctc gcgagtgtct cgactttgcc
     1561 caacaccgag cccagcaacg aattgacggt gccggtggtc tgggtgagga gattgccgat
     1621 gagtccgccg ttggcggtcc cgttgacgct tcccaacagg tcgctcacga agtcgagctg
     1681 cgcctgcttc aacgccggga tgttcgtcgc tttcgcgacg gcagcgatga gcgcgtcgcc
     1741 gatctgcgtc gcctgattca cgagctcttg ggtcgctcca gcggccgcga tgatgttctt
     1801 cacggcgtcc gcggtgagct tcgcttcgag ctgcgcggca gagtggatcg ccgcgaagac
     1861 gatcttgtcg gcttgggcca cgtttgcgag cgagttcgcg agcgtggagc tgaaggcgag
     1921 acggaccgcg acctgagcgc gaacgtgcgc cggatcaccc gtcgccgtga tgtccaccgc
     1981 gctcacgacg cccgtttgcg cgtggacggc cgcggtcact gcgttcacca gcgcctcgac
     2041 gatctgcgcg gggtcgacgc cggccgcgat cgccgacgtg atcgccttcg tcaaatcggt
     2101 gtcgatcagc gagagcacgt tttggacgag cgataggtcg acttgggagt ttagcggggc
     2161 cggtgcgacc gcgcagctcg tcgagttgac gacgacgttg gcctcgagtg acgtctcgct
     2221 cgtcatcggc ggagccacga cgttcttcgc cgcgttgttc gatgcgtcga cgacgacggc
     2281 gccgaggacg ttgcccaccg cgtccagcgc ttcggctacg agcgtgcccg cttggaccgg
     2341 gattgcgagg ctgtaagtgc cgtttgctcc tacattggtt tcgccgagca gggcgagagt
     2401 gccaccgacg agctgtgaga cacgcacggt cgcggctttc gccaaggtcc cgacgccgcc
     2461 caagccgtcg gcggggttgc ccgtgccaag atccgtgatt gtccctagaa tcgagttctc
     2521 gagcgctggt gcagccggga tcgctgtgtt gtcgggcccc ggagcggcgg gtgtgctcgg
     2581 ctcgcttgcc ggtgcgccgg gcgcgttcgt ggccacgtct accgccccat cgttcatggc
     2641 ggtcggcgtc gcttcactcg tgggtgtgtt cggcgcagcg ggtgcagacg gcgtgctcgg
     2701 tgctgccgca ttcgcgttgg gtgatgtggg cagtgacgga tcgtgaccag agggcgagcc
     2761 tccgtcgctc cccgagcaag cggtcgctgc gagaaccagt gcaagtacgg cccatcccga
     2821 gtaaactcgt ttcatctcag tcatcccttt cggtgctgtc gcaaggtttt tcggtcgtcg
     2881 cgccaactcc acatgagcaa ctcccgatcc aaggggccgc ggggcccgag aggcacgaaa
     2941 tcagctgaaa ttggcgttgc cgcccggcgt tgcgcgacgc gctcgtcacg cttcggcggg
     3001 cttcggtgcg acgcccgcgt cgcggcagag cgctggcttg gccagcaggt ggaaagggcg
     3061 tcgtgagatc acccgcggaa gatgcaacga cgcgtattcg cgccgaggtg cgtgggctgc
     3121 gcgtgctgtc gttcgatctg gtcgtcgtgg acggtccgtc gcgcggcgcg cgcgtgcaca
     3181 tcgcgggagg agtggctcgg gttggcagcg ctccgggaaa tgacttggcg ctttcggacg
     3241 ccaccgtgtc ccgcgtccat tgcgagctcc gcgcgcagtc ggacggcgtg gtcgtgaagg
     3301 attgtgggag cacgaacggt accttcgtcg aaggtgttcg cctgcgtgag ggcgacgtga
     3361 agcccgg
//
LOCUS       DASTKS010000213         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1595275, whole genome shotgun sequence.
ACCESSION   DASTKS010000213 DASTKS010000000
VERSION     DASTKS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595275"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFQ35_04465"
     CDS             complement(<1..516)
                     /locus_tag="VFQ35_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9929913.1"
                     /translation="MSNVEKASETGIRFAYWVPNVSGGLVISKIEQRTSWDLDYNLKL
                     ARLAEKNGFDYALSQIRFTAGYGAEYQHEPVAFSHALLAGTERLKLIVALLPGPWNPT
                     LAAKQLATIDILTKGRVAVNIVSGWFKGEFRAIGEPWLEHDERYRRSEEFIRALKGIW
                     TEDSFTFKGDFY"
     gene            complement(520..1119)
                     /locus_tag="VFQ35_04470"
     CDS             complement(520..1119)
                     /locus_tag="VFQ35_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9929914.1"
                     /translation="MSRLSAVAITGNLHSPSRSAALGRVVLRALESQHAFDTSLFELV
                     HVARSLGQALNRAEVSAPVELVLQAAERADVLVVVTPVHRASFSGLFKHFFDLLSLES
                     LRDVPVILAAAGGTERHSLVLEHALRPLFSFFGAQTVPTAIFATERDFDAERRATPMI
                     EERAERAAKQATALLHPPFARRLGREASVALEPNLTLEN"
     gene            complement(1116..1895)
                     /gene="ribB"
                     /locus_tag="VFQ35_04475"
     CDS             complement(1116..1895)
                     /gene="ribB"
                     /locus_tag="VFQ35_04475"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET9929915.1"
                     /translation="MSFESVREAIRAIARGELVVVADDDDRENEGDLILAASAATPEK
                     LSFMVRHTSGVICVAASSERLAELELPLMVPRNEDSHQTAFTVSVDYRHGTTTGISSA
                     DRSATIRALADTNTSPRDLTRPGHVFPLRAQPNGVLARAGHTEAAVDLAKLAGLPALG
                     ALAEVVNEDGSMARRPELVRFAREHGLVYTTIAELVIYRQWLEEAARCISSVAQAAVT
                     QQRSPIGASLAGLSTRESPSPARSAASARLLHEAALSLETP"
     gene            complement(2046..>3302)
                     /locus_tag="VFQ35_04480"
     CDS             complement(2046..>3302)
                     /locus_tag="VFQ35_04480"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9929916.1"
                     /translation="WPGNIRELENAVHHALLVCRNGRIEPSDLRLTSLPLRARQSEAT
                     LTSNIDPLESLAGVLRDLFEVGMPDLWDNIERTVMRSAYDYCERNQVQTARLLGVSRN
                     VVRARLLDYGEIPGSVRSPPPTLAAADPPAIARELRSSPRVRIGYQRFGLLPLMKAQG
                     GLDAVFAARGVRIEWLEYPGGIQLVEGFQSSGLSLGGVGEGPPVFAQAANVPIVYFAA
                     EAPAPEDEALVVPTGSNIRNVRELAGKRVALNRGANVHYLLIRALEEAGLAYEDVEVV
                     YLAPDEARSAFDRGAVDAWAIWAPFLTELVLSGSARVLRDASGLADNAVFYIAARDFV
                     TQWPELANLFFSQLAGVAATFAPHHIPRRVDESLFEGQQVVADAFHRHKLIQRAITVR
                     AAHASSTHALPSARLQSPGHSRGVAL"
BASE COUNT          564 a         1049 c         1131 g          558 t
ORIGIN      
        1 gtaaaaatcg cctttgaagg tgaagctatc ttcggtccaa atgcccttca gcgcgcggat
       61 gaactcttcg gagcggcggt agcgctcgtc gtgttcgagc cagggctcac cgatggccct
      121 gaactcgcct ttgaaccagc cgctcacgat gttcaccgcg acgcgtccct tggtgagaat
      181 gtcgatcgtc gcgagttgct tcgctgcgag cgtcgggttc cacggaccag gcaggagcgc
      241 gacgatgagc ttgagccgct cggtgcccgc gagcagggcg tgagaaaacg ccactggctc
      301 gtgctggtac tccgcgccgt agccggcggt gaagcggatt tggctgagcg cgtagtcgaa
      361 gccgttcttc tcggccaagc gcgcgagctt gaggttgtag tcgagatccc agctcgtacg
      421 ctgctcgatc ttgctgatga cgagcccgcc gctcacgttg gggacccagt acgcaaagcg
      481 aatgcccgtt tcagatgctt tttccacgtt gctcatcgtt caattctcca gggtgagatt
      541 cggttcgagc gcgacgctcg cctcgcggcc gagacgtcgc gcgaagggcg ggtgcaagag
      601 agcggtcgct tgctttgcgg cgcgctcggc gcgctcttcg atcatgggcg tcgctcgtcg
      661 ctcggcgtcg aagtcacgct ccgtcgcgaa aattgcggtc ggcaccgttt gcgcgccgaa
      721 gaacgagaac agcggtcgca gcgcgtgttc gagcaccagg ctatggcgct cggtcccgcc
      781 cgccgcggcg aggatcaccg gcacgtcgcg caacgactcg aggctcagga ggtcgaagaa
      841 gtgcttgaag agcccagaga acgaggcgcg gtgcacgggg gtgaccacga cgaggacgtc
      901 ggcacgctcc gccgcctgca acacgagctc gactggggcc gagacctctg cgcggttcag
      961 cgcctgaccg agcgagcgcg ccacgtgcac gagctcgaac agcgacgtgt cgaacgcgtg
     1021 ctgagactcg agcgcccgca acacaacgcg cccgagggcc gcgctgcgcg aggggctgtg
     1081 caggtttccg gtgatggcga cggcgctcaa tcggctcatg gcgtctccag actgagcgcg
     1141 gcctcgtgca gcaaccgtgc cgacgctgca gaacgcgctg gagacggact ttctcgcgtc
     1201 gagagtccag cgagcgacgc accgattgga ctgcgttgtt gggtgactgc tgcctgagca
     1261 acactcgaga tgcagcgcgc tgcctcttcc agccattgtc ggtaaatgac gagctcggcg
     1321 atggtcgtgt agacgaggcc gtgctctcga gcgaaccgga cgagctccgg ccggcgcgcc
     1381 atgctgccgt cctcgttcac gacttcggcg agagcgccga gcgcgggaag cccggcgagc
     1441 tttgcgaggt ccaccgccgc ttcggtgtgg ccggcgcgtg ccaacacgcc gttcggctgc
     1501 gcgcgcagcg ggaacacgtg ccccggtcgc gtcaaatcgc gcggcgatgt gttcgtgtca
     1561 gcgagagcgc gaatcgttgc gctacggtcc gcgctcgata ttcccgtggt ggtgccgtgc
     1621 cgatagtcga ccgaaacggt gaaggcggtt tggtgcgaat cttcgttgcg aggcaccatc
     1681 agcggcaact cgagctcagc gagacgctcg gaggaggcgg cgacgcagat cacgccgctc
     1741 gtgtggcgca ccatgaacga cagcttctcg ggcgtggccg cactggccgc gagaatcagg
     1801 tcgccttcgt tttctcgatc gtcgtcatcg gcgacgacga ctaactcgcc ccgagcgatg
     1861 gctcggatgg cttccctcac ggattcgaag ctcatgcgcc atggccctac gcacactccg
     1921 tgccgccgaa ttgcgccccg atgcgagcct cctcagcggg cttggaagcc cgaactgttg
     1981 ctgtagcaac atcccccgcg cagcttgttc ttcccgcagc aacgcggcgg cgttcccgtg
     2041 gccgatcaga gcgcgacgcc cctcgagtga ccaggggact gcaagcgcgc gctcgggagc
     2101 gcgtgtgtgg atgaggcgtg cgccgcgcgg acggtgatgg cgcgttggat caatttgtga
     2161 cggtggaatg cgtcggcgac gacctgctgc ccttcgaaaa gggattcgtc cacgcgccgg
     2221 gggatgtggt ggggtgcgaa cgtggccgcg acccccgcaa gctgcgaaaa gaacaaattg
     2281 gcgagctcgg gccattgggt gacgaagtca cgagcggcga tgtagaacac tgcgttgtcg
     2341 gcgagcccgg aggcatcgcg cagcacgcgc gcgcttccgg aaaggacgag ctccgtgagg
     2401 aacggcgccc atatcgccca agcgtctaca gcgcctcggt cgaacgcact gcgcgcctcg
     2461 tccggcgcaa gatagacgac ctcgacgtcc tcgtacgcga ggcccgcttc ctcgagcgcg
     2521 cggatcagca gatagtgaac gttggcgcca cggttcagcg ccacgcgctt gccagccaac
     2581 tcgcgtacgt tgcgtatgtt cgagccggtg ggcaccacga gcgcctcgtc ctcgggggct
     2641 ggcgcctctg ccgcgaagta cacgattggc acgttcgcag cctgtgcgaa taccggcggt
     2701 ccctccccaa cgccgccgag cgagaggccg ctcgactgga agccttcgac tagctgaatg
     2761 ccaccggggt actcgagcca ctcgattcgg acaccccgcg ctgcgaacac cgcgtcgagc
     2821 ccgccctgcg ccttcatgag cggcaaaagg ccgaaacgtt ggtagccaat gcgcacgcgg
     2881 ggagacgaac gcaattcacg tgctatcgcc ggcgggtcgg cggcggcaag agttggcggc
     2941 ggcgaacgca ccgagccggg gatctcgccg tagtcgagca agcgagcgcg caccacgttt
     3001 cggctgaccc cgagcaggcg cgcggtctgc acctggttgc gctcgcaata gtcgtaggcg
     3061 gagcgcatca cggtgcgctc gatgttgtcc cacagatctg gcatcccgac ctcgaacaag
     3121 tcgcgcagca cgccggcgag gctctcgagc gggtcgatgt tggaggtgag cgtcgcctcg
     3181 gattgcctcg ctcgcagggg gagggaagtg agtcggagat cggagggctc gattcgaccg
     3241 ttgcggcaga cgagcagggc gtggtgcacc gcgttctcga gctctcggat gtttcctggc
     3301 ca
//
LOCUS       DASTKS010000214         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_34035,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000214 DASTKS010000000
VERSION     DASTKS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34035"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1031)
                     /locus_tag="VFQ35_04485"
     CDS             complement(36..1031)
                     /locus_tag="VFQ35_04485"
                     /inference="COORDINATES: protein motif:HMM:NF008218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HET9929917.1"
                     /translation="MSKHPREHTYRPAWWVPGAHAQTLWAKFARPRPRLPTRIERWPT
                     PDEDFVDILRLDAAADRPRLFLLHGLEGTVRSHYVGGLFQMAWQRGWGADMLVFRSCG
                     DEPNRARRFYHSGETGDLGYCIDRVLGDFTQAPLVLCGVSLGGNVLLKWLGEHGGQLP
                     PRIRAAAAVSVPFDLERGSRHVSRGFSRIYERHFLRTLRQKAVAKHERFPDLFDLNTM
                     RRARTLYEFDDAVTAPIHGFRSAQDYYSRSSSLGYLDRIRVQTLLLSARDDPFLPAAV
                     LDEVSRIALRNASLVPEFHDRGGHVGFVGGTPWSPRYFVELRVGAFFDEALAGDR"
     gene            1082..1441
                     /locus_tag="VFQ35_04490"
     CDS             1082..1441
                     /locus_tag="VFQ35_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HET9929918.1"
                     /translation="MEVQIFGVKKSADTRKALRFFSERRIRTHFVDFAERPASTGELK
                     RFAQRFGIDALIDRESRAFADLGLRHAALSEDRWLDKLIDEPLMLRMPLVRQVGPGAK
                     GLTIGLAEDEWRQWTSN"
     gene            1455..>2104
                     /locus_tag="VFQ35_04495"
     CDS             1455..>2104
                     /locus_tag="VFQ35_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9929919.1"
                     /translation="MTQLQVSNVAVEFGATTLFSDVTFTVSAGERWGIIGRNGTGKTT
                     LFRLITGEAEPSRGAVTMQAGLRISLLEQHRDFGDATTVWEAAAGGLADLLRLEGSLL
                     EQAHALAENSSDEALARYGRDLERFEREGGYEATARIDAVLHGLGFDPQQARTTPVGQ
                     LSGGEQGRLALARELVRGGDVLLLDEPTNHLDLETTRWLEQFLREAATTVLLISHDR"
BASE COUNT          407 a          644 c          721 g          332 t
ORIGIN      
        1 cgatgctgct ccatcatcgt cgagtcggtc gcgttctacc ggtcgccggc gagtgcttcg
       61 tcgaaaaagg cacccactcg cagctccacg aaataccgcg ggctccacgg agtcccaccg
      121 acgaagccga cgtgaccgcc acggtcgtga aactccggca caagcgacgc attgcgaagg
      181 gcgatccgtg aaacctcgtc gagcaccgcg gcgggcagga atggatcgtc gcgagcactc
      241 agcagaagag tttggacgcg gatccggtcg aggtatccca gtgagctcga gcgagagtag
      301 tagtcctgag cactccgaaa cccatgaatc ggggctgtca ccgcatcgtc gaattcgtag
      361 agcgttcgcg cccggcgcat cgtgttcaga tcgaagagat cgggaaaacg ctcgtgcttc
      421 gcgacggcct tctggcggag tgtgcggaga aaatgccgtt cgtagattcg cgagaagccg
      481 cgtgacacgt ggcgcgagcc gcgctcgaga tcgaacggca ccgaaacggc tgcggcggcg
      541 cgtatccgcg gtgggagctg cccgccgtgc tctcccagcc acttcagcag cacgttgccg
      601 ccaagggaga cgccgcagag tacgagcgga gcctgggtga agtcgccgag aacgcggtcg
      661 atgcagtacc cgagatcgcc cgtttcgccg gagtgataga accgtcgggc acgattcggc
      721 tcgtcgccgc agcttcggaa aacgagcatg tcggcacccc agccgcgctg ccacgccatc
      781 tgaaacaggc cgccgacata gtgcgagcgc acggtgcctt cgagcccatg gagcaggaac
      841 agtcgcggac ggtcggccgc ggcatcgagc cgcaggatat cgacaaagtc ctcgtcaggc
      901 gtcggccacc gttcgatgcg cgtcggaagc ctgggccgag gccgggcgaa cttggcccac
      961 aacgtctgtg cgtgggcacc cggtacccac cacgcgggac ggtacgtatg ttcgcgaggg
     1021 tgcttggaca tcgaatccga gagagcgagt cgaatcgttt agccaccgag caaggtagcg
     1081 gatggaagtc cagatcttcg gtgtgaagaa gagcgccgat acgagaaagg cgctgcgttt
     1141 cttctccgag cgccgtatcc gaacacattt cgtcgacttc gccgagcgac cggcgtcgac
     1201 cggcgagctg aagcggttcg cgcagcggtt tgggatcgac gcgttgatcg accgcgagtc
     1261 gcgcgccttc gcggacctcg ggttgcggca tgcggcgctc tcggaagatc gctggctgga
     1321 caagctcatc gacgagccat tgatgctgcg gatgccgctc gtgaggcagg tcggtccggg
     1381 tgcgaaaggg ctcacgatcg gactggccga agacgaatgg cgacaatgga catcgaactg
     1441 atggcggcgg cgcgatgaca caacttcagg tgtcgaacgt cgccgtcgag ttcggagcga
     1501 cgacgctttt ctccgacgtg accttcaccg tgtcggccgg cgagcgttgg gggatcatcg
     1561 gtcgcaacgg aacggggaag acaaccctct tcaggctgat cacgggcgaa gcggagccga
     1621 gccgcggcgc cgtgacgatg caggcggggc ttcggatatc gctcctggag caacatcgcg
     1681 acttcggcga cgcgaccacg gtctgggaag ccgcggcggg aggactcgca gacctgctgc
     1741 gtctcgaggg ctcgctcctc gagcaggcgc atgcgctcgc cgagaattcg tcggacgaag
     1801 ccctggcgcg ctacgggcgc gacctcgagc gcttcgagcg agaggggggc tacgaggcga
     1861 cggcgcgaat agacgcggtg ctgcacggtc tgggattcga cccgcagcaa gcccgaacga
     1921 cccccgtcgg acagctgagc ggtggcgaac agggaagact ggccctcgcg cgcgagctcg
     1981 tgcgaggcgg cgatgtgcta ttgctcgacg agcccacgaa tcacctcgat ctcgagacta
     2041 ctcgctggct cgagcagttc cttcgggaag ctgccaccac cgtgctgttg atcagccatg
     2101 atcg
//
LOCUS       DASTKS010000215         5369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1148358, whole genome shotgun sequence.
ACCESSION   DASTKS010000215 DASTKS010000000
VERSION     DASTKS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148358"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VFQ35_04500"
     CDS             <1..1524
                     /locus_tag="VFQ35_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9929920.1"
                     /translation="SMNSEPVKFLLVDDIEENLTALEGLLRRESLELLKARSGAEALE
                     LLLVHEVALAILDVQMPEMDGFELAELMRGAEITKHVPIIFVTAGGRDPQRMFKGYET
                     GAVDFLFKPIEPHVLKSKADVFFELSRQRKELANVLRLNEMFVGILGHDLRNPLGAVL
                     TGAQLLEAQLEDATQLRVVRRMLSAGERMKAMVEQLLDLTRARLAGGLGFVGARRLID
                     VGALAKRAVEELSPSHPERPLKLELRGDAVTAGDPDRLLQLLSNLISNALHHGEHAAP
                     VWVEVEGDERHVELRVRNQGAIPAERIPTLFDPFRTGQGHGGGSKGLGLGLFISQQIA
                     LAHGGAIAITSNSGQTEVRVSIPRVAPASAPAPPPHARRLLVVEDEEQVRESLREAFQ
                     REGYDVSTAKNGREALELLAQPATRPDLVVLDLVMPVVDGGSVYRAMQQDPALSQIPV
                     VVSTSSPGRAPAGAVVVPKPLNLERLLAAVRSFWTQAASSERTDRNPLSTQSHAGKI"
     gene            1529..1819
                     /locus_tag="VFQ35_04505"
     CDS             1529..1819
                     /locus_tag="VFQ35_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9929921.1"
                     /translation="MFIIELTYKVALEKIDAQMRPHMAFLNKHYDDGTFIISGRKIPR
                     DGGIILAVGKTREELESIVREDPFVSLGLADYRIIEFRASQRAKDIPARVEG"
     gene            2179..3006
                     /locus_tag="VFQ35_04510"
     CDS             2179..3006
                     /locus_tag="VFQ35_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HET9929922.1"
                     /translation="MNLAAPRVPSFAAESTPPSSKKLKPSEEKPASSRAPATPARMAS
                     DRLRVGLLSGVAGYVDAAGFVSLLGLFPAHLTGELVGLSISLASGQPLKLRMLMLPVF
                     VFAVALSVIVVRFFRRRGQPTLLPLLTMMALALLLFSLSGFVWPELGHASHPVPILLL
                     SAVAAMAFQNTFMREAVGTSCPTTVMTGNLTAFVIELVDLFVVRAWTREPRSAGDRAK
                     ADARLKLVATALFGFTGGATLGGWLTGVVGPRSMALPTLAVIFLAESARRDAKVQDR"
     gene            complement(3024..3770)
                     /locus_tag="VFQ35_04515"
     CDS             complement(3024..3770)
                     /locus_tag="VFQ35_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929923.1"
                     /translation="MARLVPLDLLMVSTSAHRMGVVGALGTLAGIALSGPIAVGIVSA
                     TRPQPPWSGADVFSAHYHPLQLLPYAGGLLLVVGLLVVVACAHALADEGSKAHTSLGI
                     LFASVFATMIFGNYTIQSAFIPVLVREGGAGNAALIAALSMSNPRSLAWALEMWGWGF
                     CGAATWCIAPVFASRWLRRVAFANAVVSIAGALLTSAAPGWVLTAAGIAAFLFWNALL
                     CLLALLWLAAFRGASRSTGASLARAVPRGV"
     gene            3982..>5369
                     /locus_tag="VFQ35_04520"
     CDS             3982..>5369
                     /locus_tag="VFQ35_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929924.1"
                     /translation="MTQFVPVKSIARHVSPLALLALLSAPLQGCGSDEGGGNSEGGAT
                     ALGGKASGGALNGGSPGSGGATSGGTGSGASNGGAMSAGGTTSSAGSANGGAKSSGGT
                     SNNGSGGSSSKGGASATAGAGGASKGGAAGAGGKTASGGVTGSGGNSSGGAASGGTKS
                     SGGATGSGGSSGSCSAPPTFDGVVGWAATSASGVTTTTGGGNATPVTVTTLAALQEAV
                     KGTNAAVIYVKGSLSAGNINVGSNKTIAGVCGGEIHGHLEMGGSVNVILQNIKVVGYG
                     VGNCSLDPSYDPSVGCSSGQDAISIQKNAHHVWIDHADISDGTDGNLDITNGANYVTV
                     SWTKFHYTARTDDSGDDSTGAAGHRFSSLVGGTDNPSSYDDANALNVTWHHNYWADRV
                     VERQPRVRFGKNHLFNNLWASSGDNYCVRAGKQAKLLLENNVFQGVKNPQQFNNSTDE
                     GTAFITATGNVYT"
BASE COUNT         1046 a         1714 c         1758 g          851 t
ORIGIN      
        1 agtatgaatt cggagccagt caaattcctt ctggtcgacg atatcgaaga gaacctgacc
       61 gcgctcgaag ggttgctccg acgggagagc ctcgagctct tgaaggcgcg ctccggtgca
      121 gaggcgctcg agctactctt ggtacacgaa gttgcgctcg caattttgga cgtgcagatg
      181 ccggagatgg acggcttcga actcgcggag ctgatgcgtg gagccgaaat cacgaagcac
      241 gtgccgatca tcttcgtgac cgcgggcggc cgggacccgc agcgcatgtt caaaggctac
      301 gagaccggcg cggtcgattt cctcttcaag cccatcgagc cgcacgtatt gaagagcaaa
      361 gccgacgtct tcttcgagct ctctcggcaa cgcaaggagc tcgccaacgt gctccggttg
      421 aatgaaatgt tcgtcggcat cttggggcac gatcttcgaa acccgctcgg agccgtgttg
      481 accggagcgc agctgctcga ggcgcagttg gaggacgcga cccagctccg cgtcgtgcga
      541 cgcatgctct ccgcggggga gcgaatgaag gcgatggtcg aacagctgct ggacctgact
      601 cgggctcgcc tcgcgggggg actcggtttc gtcggggcgc gtcgcctcat cgacgtcggc
      661 gcgctcgcca agcgcgcggt cgaggagctc tcgccctcac atcccgagcg gcccttgaag
      721 ctcgagctgc gcggcgacgc ggtgacggcg ggcgaccccg atcggctgct ccagctgctc
      781 tcgaacttga tctcgaacgc gcttcaccac ggcgagcacg ccgcgccggt ttgggtcgaa
      841 gtcgaaggcg acgagcgcca cgtcgagctt cgggttcgaa accagggcgc tattcccgcc
      901 gagcgcattc cgactttgtt cgatccgttt cggactgggc aggggcacgg aggcggctcg
      961 aaagggctcg gactcggact cttcatttcg cagcaaatcg cgctcgcaca cgggggcgcc
     1021 atcgccatca cgtcgaactc cggacagaca gaggtccgcg tgagcatccc ccgcgtggcc
     1081 cccgcgagcg cgccggctcc gcctccgcac gcgcgccggt tgctggtagt ggaagacgag
     1141 gagcaagtgc gcgagtcgct gcgagaggca ttccaaagag agggttacga cgtcagcacg
     1201 gcgaagaatg gccgcgaggc gctagagctg ctggcgcagc ccgcgacacg cccggacctc
     1261 gtcgtcctcg atctcgtcat gcccgtcgta gacggaggct ccgtctaccg cgcgatgcag
     1321 caggacccag ccctgtcgca aatccccgtc gtcgtatcga cttcgagccc ggggcgggct
     1381 cccgcaggcg cagtcgtggt cccgaaaccg ctgaacctcg agcgcctgct cgcggccgtg
     1441 cgtagcttct ggacacaagc ggcgagctcg gagcggacgg accgaaatcc actgtcaacc
     1501 cagagccacg ctggcaagat atgaggccat gttcatcatc gagctcactt acaaagtcgc
     1561 cctggagaag atcgatgcgc agatgcgccc gcacatggcc tttctgaaca agcactacga
     1621 cgacggcacc ttcatcatct ccggccgcaa aatccctcgt gacggcggca tcatcctcgc
     1681 ggttggcaag acccgtgaag agctcgaaag catcgtgcgc gaagatccgt tcgtcagcct
     1741 cggcctggcc gattaccgga tcatcgagtt tcgcgcgagt caacgcgcaa aagacatccc
     1801 cgcacgcgtc gaagggtgag agtcgggcga acgcgttgaa agcgcgtgta atacgcgagt
     1861 acgaagatcg ccgcgtaacg aaactcacaa tcgcgcgggt atacgaaact cgatcggatt
     1921 agaacgcacg aagccactta agctcgaccc gcccctcgcc gttcactccg gagaggtaag
     1981 ggagtggcac tgagttctcg ctggtcgcgg agaatcaaag cctcgagcgc gaggccgctc
     2041 cctgccggcg cgccgaccga gcggcgatcc tcgggcgtga tggcgatacg cgcgaggcgt
     2101 cacgcgactt tgagtgttcg tcccagttct tggcctgcgc cgcgcgcgag cgattcagaa
     2161 agatcgctgc ctatcggggt gaatttggcc gcaccccgtg tccccagctt cgccgcggag
     2221 agcacgcccc caagctcgaa gaagctcaag ccgagcgaag agaagccggc gagctcacgc
     2281 gcgcccgcaa cccctgctcg gatggcgagc gatcgcctgc gcgtcggact gctgagcggc
     2341 gttgcaggct acgtcgacgc ggcgggattc gtcagccttt tgggtttgtt ccccgcgcat
     2401 ctaaccggtg agttggtagg gctcagtata tcgctcgctt cggggcaacc gctcaagttg
     2461 cgcatgttga tgctgccggt gttcgtgttc gcggtcgcgc tgtccgtcat cgtcgtccgc
     2521 ttctttcgac gacgagggca accgaccctg cttcctttgc tgacgatgat ggcgctcgcg
     2581 ttgctcctct tttcgttgag cggtttcgtg tggccagagc tcgggcacgc ctcgcacccg
     2641 gttccgattc tgctgctcag cgcagtcgcg gcgatggcgt ttcaaaatac gttcatgcgc
     2701 gaagcggtcg gcacgagttg tccaaccacg gtgatgaccg gcaacttgac cgccttcgtg
     2761 atcgagctcg tcgacctctt cgtcgtccgc gcctggacgc gagagccgcg cagcgccgga
     2821 gatcgagcca aggccgacgc gcgactgaag ctcgtcgcca cggcgttgtt cggcttcact
     2881 ggcggcgcga ccctcggcgg gtggctcacc ggggtcgtcg gtccgcggag catggcgcta
     2941 ccgacgctcg ccgtaatctt cctggccgag agtgctcgac gcgacgccaa ggttcaggat
     3001 cgctagggtc ggtcgcggcg acttcagacg ccgcgcggca ccgctcgcgc gagcgacgcg
     3061 cccgttgacc tcgatgctcc tcgaaacgcg gctagccaga gcagggcgag caggcagagc
     3121 agcgcgttcc agaacaaaaa agccgcgatc cccgcggcgg tcaggaccca gccgggcgcg
     3181 gcgctggtca ggagcgcccc cgcgatgctc acgaccgcgt tcgcgaacgc cacgcgtcgc
     3241 aaccagcgcg acgcgaacac cggcgcgatg caccacgtcg ccgcgccgca aaatccccaa
     3301 ccccacattt ccaaagccca ggcgagcgac cgtgggttcg acatcgagag cgcggcgatg
     3361 agcgccgcat tcccggcgcc gccttcacga acgaggactg gtatgaacgc ggactgaatc
     3421 gtgtagttgc cgaagatcat cgtcgcgaac acgctggcaa acaagatgcc cagactcgtg
     3481 tgtgctttcg agccctcgtc ggcgagcgca tgcgcgcaag cgacgacgac cagcaaaccg
     3541 acgacgagca gtaagcctcc cgcataaggc aggagctgca gcgggtgata atgcgccgag
     3601 aagacgtcgg caccactcca gggcggctga ggccgggtcg cgctcacgat gccgacggcg
     3661 atggggcccg agagcgcgat gcctgccaga gtgccgaggg cgccgaccac tcccattcga
     3721 tgtgcggacg tgctcaccat gagcaggtcg agaggcacca atcgcgccac ccgaaacctc
     3781 cgaaaagctc agctttcgca gagaaatgcg gatgcaagga aatcgcgctt ccgcaaacgt
     3841 tgcgctcggc cgcacgcgcc gatcggattg aaaattcttg cctcgacggt gtccggttcc
     3901 gttcgagctg ggcccggtga gccgcggcgc gagttccagg caactggtat gtcgcgcgct
     3961 cgagcgcaga gcaccgtaga catgacacag ttcgttcccg tgaaatccat cgctcgacac
     4021 gtgagtcccc tcgccttgtt ggcgcttctt tcggcgccgc tccaaggctg cgggtcggac
     4081 gagggaggcg gcaactccga aggcggagcg acggcactcg gaggaaaagc ctctggagga
     4141 gctctgaatg gcggctcgcc gggaagcggc ggcgcaacga gcgggggaac gggcagcgga
     4201 gcaagcaacg gtggagcgat gagcgctggc ggcacgacga gcagcgccgg cagcgcgaac
     4261 ggcggcgcga agtcgagcgg aggcacgagc aacaacggga gcggcggctc gtcgagcaaa
     4321 ggcggagcga gtgcgaccgc gggcgcaggc ggagcgagca aaggtggagc agcgggcgca
     4381 ggcggaaaaa ccgcgagcgg aggcgtgacc ggcagcggcg gaaacagcag cggtggtgct
     4441 gcgagcggcg gaaccaaatc gagcggagga gcgaccggaa gtggcggcag cagcggctct
     4501 tgcagcgcgc ctcctacttt cgacggtgtc gttggttggg cggccacgtc cgcgagcggc
     4561 gtgaccacca cgaccggagg tggcaacgca acgccggtca cggtgacgac gctcgctgcg
     4621 ttacaagaag cggtgaaggg caccaatgcg gcggtcattt acgtgaaggg ctccctcagc
     4681 gcgggcaaca tcaacgtcgg ttcgaacaag acgatcgctg gtgtttgcgg cggggagatc
     4741 cacggacacc tcgagatggg cggctcggtg aacgtgatcc ttcaaaacat caaagtggtg
     4801 ggatacggcg tcggcaactg ctcgctcgat ccgagctacg acccttcggt tgggtgctct
     4861 tcgggccaag acgcaatttc gatccagaag aacgcgcacc acgtctggat cgatcacgcc
     4921 gatatctccg acggcacgga cggcaacctg gacatcacga acggagcgaa ctacgtcacg
     4981 gtctcgtgga ccaagttcca ctacacggct cgtaccgacg acagcgggga cgattcgacg
     5041 ggcgcggcgg gtcaccgctt cagcagccta gtcggcggaa ccgacaaccc cagcagctac
     5101 gacgacgcca acgcgctgaa cgtcacctgg caccacaact attgggccga tcgcgtggtc
     5161 gaacgtcagc ctcgcgtgcg cttcggtaag aaccacctct tcaacaacct ttgggcttct
     5221 agcggcgaca actactgcgt ccgggccggc aaacaagcca agctcctcct cgagaacaac
     5281 gtgttccaag gagtgaagaa cccgcagcag ttcaacaatt ccaccgacga aggcaccgcc
     5341 ttcattaccg cgaccggaaa cgtttacac
//
LOCUS       DASTKS010000216         8589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1372025, whole genome shotgun sequence.
ACCESSION   DASTKS010000216 DASTKS010000000
VERSION     DASTKS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372025"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFQ35_04525"
     CDS             complement(<1..936)
                     /locus_tag="VFQ35_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9929925.1"
                     /translation="MPDFIEIAKLDATAQAELVRSGALSGDELLSACEARVRLLEPTV
                     RAFSSINFEKARARLSAEPVRTGTFAGVPLAIKDVAPYPGFPWALGSRLLAKNLVTAP
                     SPLVQRLEEAGFVVFAKSTTSEFGLLGSTETLLAGVTHNPWDLSRSALGSSGGAAAAV
                     AAGLIPLAHASDGGGSIRIPASACGVFGFKPSNGRNVSPSIAGAGGGSDFLKLTPDHC
                     ISRSVRDSARFLAVLERTELEPSLPRVGEVTRPIERRLKLGYYSRNLFQVEPSEPIRI
                     ALDRTVALCASLGHEVVEIPAPVVDAPGISEAFFTV"
     gene            complement(1136..3178)
                     /locus_tag="VFQ35_04530"
     CDS             complement(1136..3178)
                     /locus_tag="VFQ35_04530"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET9929926.1"
                     /translation="MKSSLSPSAFRLGSRAVGLLGVTAFFFALGTGLVRSSTFSTAYF
                     WRNEVAQSERLALLAYALALPVIAALAGAAAFIYSYPRGVAAAEKLYRVAVRLSPLGP
                     LAMVPWLLRWDIWVGHDLAFLCAAMLVTLALGRALTKAIETLPLHEASPLYLLARRRH
                     SVHAGTGNFISREPFLSAAVLAALLYITWFSYYTVVFHLSCRSGWDTSIEDNILWNIS
                     HGGPFFKASPALGPTGAHFRRHATLISYLLAPFYLLKPGAATVLVIQSTLQGLAAVPL
                     FLFARRHVGQGFALLVSIAYLFHPALQESNLFEVHYLKFGPVFLFTALWLLDSGRNRL
                     ALLAAVLTMLVREDVASWVILLGLWGLMTGRDPRYSGGIAALGCVYVGLIKFVAMPAA
                     GHGQDELTFIYSELVPKGSQGFGAVLGTVFGNPAFTLESLFDPAKLLYFLQLFLPLAF
                     IPLRRTIGWLALIPGGIYCFISTHYSPFVDIHFQYSAHILTFAFPALVLVLADSTPVV
                     ERPDTDSLPALSRAAALRHSLARRYGFAASMLVATLIGTYQYGAILQQNTSRGGPIPY
                     TFGWNDEGRARRHSIDELLKVIPPDAKVTASAFTVPQISARSDAYSLSISIYDADFIL
                     APSGPKEHLPDEKNRIREVLKSGEFGVLKVVPPFFVAKRGAPTKDNESLLRDINAL"
     gene            complement(3186..4268)
                     /locus_tag="VFQ35_04535"
     CDS             complement(3186..4268)
                     /locus_tag="VFQ35_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9929927.1"
                     /translation="MSSEEAAPAPLPAPEGSAPVASAPAAQGPAPVAPRRPRPVWEWF
                     WRGHALGEARREIDGQRSRYGVFEARARAAAEFGRRALDPGEARAAGTADALASELYV
                     QAAYWALLALAGTDANTPASKLPFDLTAAMALVDREVLLRAAGSALALDAIENAAALS
                     DYFEAELTDQAHVALTLRNFTESLLTLLSRNEQRVWLLRVQRLTRVGALFVVGVGLLV
                     TALLVADWAEQRRDLARDKAWHASSVGASSCNSPAQFCDESPDFFFHTAEERNPWLEI
                     DLGAPTQFSAVRLINRRDCCRDRASPIVIEASDDQKAWREIARHEGTFSKVKLSFPSV
                     RARYLRVRIPPPKLTNLHLAGVRVLP"
     gene            complement(4358..6355)
                     /locus_tag="VFQ35_04540"
     CDS             complement(4358..6355)
                     /locus_tag="VFQ35_04540"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET9929928.1"
                     /translation="MKAAQPAAENRIAVLDPSLSLALFVRTLGLLFGEGFSLGLLVWS
                     LAMGGGVIAYAADNRASLLERSVLIASMFGTGALFTIVGSAWLVLGKRPDLARLNGIA
                     RRLSPLMLSGFLPFLFQWQLWVARELTYAVFASFLGFGVHAAVLTSLKAPPLFGAHST
                     ELSRAWQRLGRKLEGFLPITLVLLGIVLYASFFSFYTLRNHWALRTASLDLGLEANLV
                     WNLIHGGQFMKSSPLVGPVGSHFGYHATIFAYVIGIFYAAFPRPETLLVFQSIMIGAA
                     ALPLYLLGRRYVGAWTACVLALCYIFYPPVHGSNLYDFHYLPLGIFFIWLTLFLVESG
                     RYRWAVLAAILTLSVREDVASGLGLVGAYLLLSGRKPRAGLVLAIVGFGYTLVMKALV
                     MPRALHGDTSFVYMYQGLVPAGEHGFGGIMKTALANPIFTLTSLLDRDKLLYVAQIGA
                     PLCFFPWRRPIGLLCSVLGIFFTLLSTGDLPLIQISFQYTAHWTTFLFVAVLSNLVWI
                     RKPRYEGDVEGPTRQRAWVISIALLTLFTSYQYGAIFQQNTARGGFGPYNFGLSAEER
                     LRHEQLMKLVAKVPPMAKIVSSENIVPQVCSRPDSYTLRIGIFDAEYLLFSLPAGGEE
                     AANLRKAFADGFGVMEVEGPFVLAKRGYPPTKNASVVSRIR"
     gene            complement(6352..7338)
                     /locus_tag="VFQ35_04545"
     CDS             complement(6352..7338)
                     /locus_tag="VFQ35_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9929929.1"
                     /translation="MPKAPRPRPVDLAWAASSRSEQTLNLERAARLALELGDQALHPR
                     RRGESKELALACDLYRRSAACSLRALALVPSSNAEVNDAIAREPRSSEAELQNVVEVI
                     PPALLIEAAGSEERAHALSQVVAFRKFRSFATSDAAELTALAPELANFARKLLAALEA
                     RHWRIERPAVLRWLKLAALVVLATLAVVALVFGPEKWEASRDLAAGAPWKASSFYTGG
                     CDSPTQDCAAGTTFFVHTLEEENPWLEFDLGNARSVSGVRVVNRTDCCIERARPLVVE
                     VSSDHQHWREVARNTEKFTDWKTSFPTTQARWVRFEIPKRTNLHLRRVRIFP"
     gene            7618..8328
                     /locus_tag="VFQ35_04550"
     CDS             7618..8328
                     /locus_tag="VFQ35_04550"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9929930.1"
                     /translation="MLASEPTPASVSRLVAAYWSFRGAAARHRVLPDGSIDFLFDLES
                     GSGFVVGPMRSAQVLELPASTSLFGVRFVPGAASAFVDVEASALLDLDAPLGELTRAP
                     EFHLAERVAEARSDAERAALVTRVLCDTSFRLRPLDARVQSAVARLATPAHGVSTVAD
                     QIGIGERQLERLFRERVGLGPKRYARIARLQRTLSLAERERSTHANLARRAGYSDESH
                     FVREVRELAGVAPRELFP"
BASE COUNT         1617 a         2667 c         2980 g         1325 t
ORIGIN      
        1 gaccgtgaag aaggcctcac tgatgcctgg cgcgtcgacg acgggcgcgg ggatctctac
       61 gacctcgtgg cctaggcttg cacacaaagc aaccgtccga tcgagcgcga tccggatcgg
      121 ctcgctcggt tcgacttgaa acagattccg cgaatagtag ccgagcttca gccggcgctc
      181 gatgggccga gtcacttcgc cgactcgcgg tagcgacggt tcgagctcgg tacgctccag
      241 caccgccaag aaccgcgcgc tatctcgcac gctgcggctg atgcagtggt cgggcgtcag
      301 cttcaggaag tcggagccac cgccggcgcc agcgatcgag ggagagacgt ttcgaccgtt
      361 gctgggcttg aagccgaata caccacacgc cgacgcggga atgcgaatcg agccaccgcc
      421 gtcgctcgcg tgagcgaggg gaatgagacc cgccgcgacg gcagccgcgg ccccgccgga
      481 agacccgaga gcggagcgcg acagatccca cggattgtgc gtgacgcccg ccagcaacgt
      541 ttcggtgctg ccgaggagcc cgaactcact ggtggtgctc ttcgcgaaaa ccacgaaacc
      601 tgcctcttcg agccgctgga cgagcgggga cggcgcagtc accaggttct tggcgagcaa
      661 tcgggaaccg agcgcccacg gaaagcctgg ataaggggcg acgtctttga tcgcgagagg
      721 cacccccgca aaggtaccgg tgcgcacggg ctcggccgag agccgggctc gcgccttctc
      781 gaagttgatg ctcgaaaacg cgcggaccgt gggctcgagc aagcgcacgc gcgcttcgca
      841 cgcgctcaag agctcgtccc cgctgagtgc tccggagcga acgagctcgg cctgcgcagt
      901 cgcgtcgagc tttgcgattt cgatgaagtc cggcattcgc gttctcctca aaaccgaacg
      961 tagcattcgg ttttggggca gcaagcgaga aaccaaaacg gcgccaggac cgcgattcag
     1021 gcgcactgat tcgaggcggt gagccggcac agggaagggg cgagcgcagc gttcttggcg
     1081 ctgagagcgg aattgcggtg gccgaagccg cggtgggcga acgtcgtcgc ccgtttcaga
     1141 gagcgttgat gtcgcggagc aagctctcgt tgtctttggt gggcgcgccg cgcttggcga
     1201 cgaagaaggg cgggacgacc ttgagcacgc cgaactcgcc actcttgaga acttcgcgga
     1261 tgcggttctt ctcgtcgggg aggtgctctt tgggaccgct cggcgccagg atgaagtcag
     1321 cgtcgtaaat gctgatcgag agactgtaag cgtccgaacg cgccgagatc tgcgggacgg
     1381 tgaacgcgga ggcggtgact ttggcgtcgg gcggaatcac cttgaggagc tcatcgatgg
     1441 agtggcgtcg agcccgcccc tcgtcgttcc agccaaacgt gtacggaatt ggcccgccgc
     1501 gcgacgtgtt ctgctgcagg atcgcaccgt actgatacgt accgatgagc gtcgcgacga
     1561 gcatcgacgc ggcgaaccca tagcggcgcg ccagggagtg gcgtagcgcg gctgctcgcg
     1621 acagggcggg gagcgaatcc gtgtccggcc gttcgacgac gggcgtcgaa tcagccagca
     1681 ccagcacgag cgccgggaac gcgaacgtca ggatgtgggc ggagtattgg aagtggatgt
     1741 ccacgaacgg cgagtagtgc gtggagatga agcagtagat tccacccggg atgagcgcga
     1801 gccagccgat cgtgcggcgc agcggaatga acgcgagcgg caaaaacagc tgtaagaagt
     1861 aaaggagctt tgcgggatcg aagagcgact cgagcgtgaa ggctggattg ccgaataccg
     1921 tcccgagcac cgcgccgaag ccctggctgc ccttcggcac gagctcactg tagatgaagg
     1981 tgagctcgtc ctggccgtgg ccggcggccg gcatcgcgac gaacttgatg agcccgacgt
     2041 agacacaccc gagcgcggcg atgcctccgc tgtagcgggg gtcgcgcccg gtcatcaatc
     2101 cccacagccc cagtaagatc acccagctcg cgacgtcctc tcgaaccagc atcgtgagca
     2161 ccgccgccag caacgcgagc cggttacggc cgctgtcgag tagccagagc gcggtgaaaa
     2221 ggaagacagg tccgaacttg aggtagtgga cctcgaatag gttgctctcc tggagcgcgg
     2281 ggtggaacag gtatgcgatg ctgacgagca gcgcgaagcc ttgcccgacg tggcgccgcg
     2341 caaagaggaa caggggaacg gcggcgagcc cttgcagcgt ggactgaatg acgagcaccg
     2401 tcgccgcgcc gggcttcagc agatagaacg gtgcgaggag atagctgatc agcgtcgcgt
     2461 ggcgccgaaa gtgcgcgccg gttggcccga gcgcagggct cgctttgaag aatggcccgc
     2521 catgcgagat gttccacagg atgttgtcct cgatggacgt gtcccacccc gagcggcacg
     2581 acaggtggaa caccaccgtg tagtagctga accaggtaat gtagaggagc gcggcgagaa
     2641 cggcggcgct gaggaagggc tcacgggaga tgaagtttcc ggtacctgcg tgaacggagt
     2701 gccttcgccg agccagcaga tagagcggcg aagcttcgtg cagaggtaac gtctcgatcg
     2761 ctttggtcag ggctcgtccg agcgcgagcg tgaccaacat cgccgcgcac aaaaaggcga
     2821 ggtcgtgtcc gacccaaatg tcccagcgga ggagccacgg caccatcgcg agcggcccca
     2881 agggcgagag gcggaccgcg actcggtaga gcttctctgc cgcggcgacg ccgcgagggt
     2941 acgagtagat gaacgcggcg gcgcccgcga gcgcagcgat gaccggcagc gcgagcgcgt
     3001 acgcgagcaa cgcgaggcgc tccgactgcg cgacctcgtt cctccagaag tacgcggtcg
     3061 agaaggtcga ggagcgaacg agccccgtcc cgagagcaaa gaagaaggcc gtaacaccca
     3121 aaagcccgac ggcgcgcgag cctagccgga acgcgctggg ggatagcgag ctcttcatgg
     3181 ccgagttagg gcaggacgcg aacgccagcg aggtgtaggt tcgtgagctt gggcggcgga
     3241 atgcgaacgc ggaggtaacg cgcgcgcacg ctcgggaagc tcaacttgac cttggagaag
     3301 gtgccttcgt gccgtgcgat ttctcgccaa gccttttggt cgtcgctcgc ttcgatcacg
     3361 atgggcgagg cacggtcacg gcagcagtca cgacggttga tgaggcggac cgccgagaat
     3421 tgggtcggag ccccgaggtc gatctcgagc caagggtttc gctcctccgc cgtgtggaag
     3481 aagaagtcgg ggctctcgtc gcaaaactgc gcgggcgagt tgcaggacga cgcgcccacc
     3541 gagctcgcgt gccaggcctt gtcacgcgcg aggtcgcgcc gttgctcggc ccagtcggcg
     3601 acgagcagcg ccgtcacgag caggcctacc cccacgacga acagcgcgcc gacgcgcgtc
     3661 aagcgctgca ctcgcaggag ccaaacccgt tgctcgttgc ggctcaggag ggtcagcagg
     3721 ctctccgtga aattccgcag cgtgagggcg acgtgcgcct gatcggtgag ctcggcttcg
     3781 aaatagtcgc tcagcgccgc cgcgttttcg atcgcgtcga gcgcgagggc gctgccggcg
     3841 gcgcgcaaca gaacctcccg atcgacgagc gccatcgcgg ccgtgagatc gaacggcagc
     3901 ttgctcgccg gcgtgttcgc gtccgtaccc gcgagcgcga ggagcgccca ataagccgcc
     3961 tgcacgtaga gctcactcgc gagcgcgtcg gccgtgcccg ccgcgcgagc ctctcccgga
     4021 tcgagggcgc gtctgccaaa ctcggccgcc gcgcgcgcac gcgcctcgaa cacgccgtag
     4081 cgcgagcgct ggccgtcgat ttcgcggcga gcctcaccga gcgcgtgccc gcgccaaaac
     4141 cactcccaga ccggtcgcgg tcgacgggga gcgactgggg cagggccttg ggcagccggt
     4201 gcggaagcga cgggagcgct cccttcgggt gccggtagcg gcgcgggtgc ggcctcttca
     4261 ctagacatcg tagttacgct cgcccgacat ggttcacccg gtcagcgtca ttagcaaaga
     4321 tcgcgcgcgg cggctagcaa gcccctccat cagcccatca gcgaatgcgt gaaaccacgc
     4381 tcgcgttctt ggtgggtggg tagccccgtt tggcgaggac gaacgggcct tcgacctcca
     4441 tgacaccgaa gccatccgcg aacgccttgc gcaaattggc ggcttcctct ccgcccgcag
     4501 gaagggaaaa tagcaggtac tccgcgtcga agatgccgat gcgcagcgtg tatgaatcgg
     4561 ggcgactgca gacctgcggc acgatgttct cggagcttac gatctttgcc atgggaggca
     4621 ccttggcgac gagcttcatg agctgctcgt ggcgcaaccg ctcttctgcg ctgagaccga
     4681 agttgtaggg cccgaagccg cctcgcgccg tattctgctg gaagatggcg ccgtactgat
     4741 acgaagtgaa cagcgtgagc agcgcgatcg agatgaccca cgcgcgttgt cgagtgggtc
     4801 cctcgacgtc accttcgtat cgaggctttc ggatccagac caggttcgag agcaccgcaa
     4861 cgaagaggaa cgtcgtccaa tgcgccgtgt actgaaaaga aatctggatg agcggtaggt
     4921 cgccggtcga gagcagcgtg aagaagatgc cgagcaccga gcagagcagg ccgatcggac
     4981 gccgccaggg gaagaagcaa agcggagccc cgatctgcgc gacgtagagg agcttgtcgc
     5041 ggtcgagcaa gctcgtcagc gtgaagatcg ggttcgcaag cgcggtcttc atgatgccgc
     5101 cgaagccgtg ctcgccggcg ggcaccagcc cctggtacat gtagacgaac gaggtgtcgc
     5161 cgtgcagcgc tcgcggcatg accagcgcct tcatcaccag cgtgtaaccg aacccgacga
     5221 tcgcgagcac gagccccgcg cgcggtttgc ggccagacaa cagcaggtac gccccgacga
     5281 ggccgagccc cgacgccacg tcctcacgca ccgagagggt caggatcgcg gcgagtacgg
     5341 cccagcggta tcgccccgac tccaccaaaa acaaggtgag ccagatgaag aagataccga
     5401 gcggtaagta gtgaaagtcg tagaggttcg agccgtggac cggcggatag aagatgtaac
     5461 agagcgcgag cacgcacgcg gtccacgcgc ccacgtagcg ccggccgagt agatagagag
     5521 gcagcgccgc ggctccgatc atgatcgact ggaagacgag cagcgtctcg gggcgcggaa
     5581 acgccgcata aaagatgccg atgacgtagg cgaagatcgt cgcgtggtac ccgaagtgcg
     5641 aaccgacggg gcccacgagc ggcgagctct tcatgaactg accgccgtgg atcaggttcc
     5701 aaacgaggtt cgcctcgagg ccgagatcga ggctcgcggt gcggagcgcc cagtgattgc
     5761 gcagcgtgta gaaagaaaag aagctcgcgt agaggacgat gccgaggagc acgagcgtga
     5821 tgggcaggaa tccctcgagc ttcctaccga ggcgttgcca tgcgcgcgag agctcggtcg
     5881 agtgcgcgcc gaacaggggc ggtgccttca gcgacgtgag cacggccgcg tgaacaccga
     5941 agccgaggaa cgacgcgaac acggcgtagg tcagctcgcg cgcgacccag agctgccact
     6001 ggaacaggaa cggcaaaaag ccgctcaaca tcagcggcga gagacgtcga gcgatgccgt
     6061 tcaagcgcgc gaggtccggg cgcttgccga gcacgagcca ggcgctgccg acgatggtga
     6121 agagcgcgcc ggtcccgaac atcgacgcga tcagcaccga gcgttcgagc agggaggcac
     6181 ggttgtcggc cgcgtacgcg atcacgccgc cgcccatcgc gagcgaccaa acgagcaagc
     6241 cgagcgagaa gccttctccg aagaggaggc ccaacgtgcg cacgaacagg gcgagggaca
     6301 gcgaggggtc gagtacggcg atgcgattct ccgccgccgg ttgagctgct ttcatgggaa
     6361 aatccgaacg cggcgaaggt gcaggttggt ccgcttgggg atttcgaatc gcacccagcg
     6421 cgcctgagtg gtcggaaaac tcgtcttcca gtcggtgaac ttttcggtgt tgcgcgccac
     6481 ctcacgccag tgctggtgat cgctgctgac ttcgacgacg agcgggcgag cgcgctcgat
     6541 gcagcaatcg gtgcggttta cgacgcgcac cccggaaacg gaccgggcgt tgccgaggtc
     6601 gaactcgagc catgggttct cctcctcgag cgtgtgcacg aagaaggtag tcccggcagc
     6661 gcagtcctgc gtcggcgagt cgcatccgcc cgtgtagaac gagctcgctt tccacggagc
     6721 tcccgcggcg agatcccggc ttgcttccca cttctccggc ccgaacacga gggcgacgac
     6781 ggcgagagtg gcgagcacga ccaacgccgc gagcttcaac cagcgcagca cggctggacg
     6841 ctcgatgcgc cagtgacgag cctcgagggc ggccagcagc ttgcgcgcga agttcgcgag
     6901 ctcgggcgcg agcgcggtca actcagccgc gtcgctcgtc gcgaagctac gaaatttccg
     6961 aaacgccacc acctgcgaga gcgcatgggc gcgctcctcg ctgccagcgg cctcgatcaa
     7021 gagcgcgggc gggatgactt cgacaacgtt ctggagttct gcttcgctgc ttcggggttc
     7081 acgggcgatc gcgtcgttga cctcggcgtt gctcgagggg acgagcgcga gggccctcag
     7141 agagcaagcc gccgaacgcc gatacaggtc gcaggcaagg gcgagctcct tggactctcc
     7201 acgccttcta gggtgcagcg cctgatcacc gagctcgagg gcaagccgcg ccgctcgttc
     7261 gaggttcagg gtttgctccg agcgagagga agcggcccaa gccagatcga cagggcgcgg
     7321 tcgaggcgct ttcggcacgg ctcgcggttc gggcagcgag gcggccgtgg tgggtgacgc
     7381 gccttcgctc gggatggccg cttgggcagc aacttggctt gcaacctcag acgacggctc
     7441 agtatttccg ttcgggtcca cgggggcgcg cagtctagcc caaggtgagt gcccggggca
     7501 tacgcttttc gcctttggtg gaacggtgac cgttcccaag cttaagggca tgaagtccct
     7561 cgggaatcac agcggcagcg cgccgggcgc ggctcgtgtc actctggtcg acaggcaatg
     7621 ctggctagcg agcccactcc tgcatccgtc tctcgcctcg tcgcggctta ctggagcttt
     7681 cgcggcgcgg cggcgcggca tcgggttttg ccggacggga gcatcgactt ccttttcgac
     7741 ctcgagagcg gctccgggtt cgtcgtcggg ccaatgcgtt cggcgcaggt gctcgagctc
     7801 ccagcgtcga cctccctgtt cggcgtgcgc ttcgtgcccg gcgccgcgag cgccttcgtc
     7861 gacgtcgagg cgagcgcctt gctcgacttg gacgcgcccc tcggtgagct cacgcgcgct
     7921 cctgagtttc acctagccga gcgagtcgcg gaggctcgga gcgacgccga acgcgccgcg
     7981 ctcgtcacgc gggttctgtg cgacacgtcg ttccggcttc gcccgctcga cgcgcgggtc
     8041 cagtccgcgg tcgcccggct tgccacgccg gcgcacggtg tgtcgacggt tgccgaccaa
     8101 atcggaatcg gcgagcgcca gctcgagcgc ttgtttcgtg agcgggtcgg attggggccc
     8161 aagcgctacg cgcgcatcgc tcgcctgcag cgcacacttt cgctcgctga gcgggagcgt
     8221 tccacgcacg caaacctcgc tcggcgcgct gggtactcgg acgagtcgca cttcgtgcgc
     8281 gaagtgcgcg agctcgcggg cgtggcaccg cgcgagctct tcccttagca gggtgttgaa
     8341 aaacggcagc ggctcgggct cgtcgttgcg ctcctcgacc tgcttcagcc tcaggattcg
     8401 tcacatgtcg cgagcaggta ggccttcgtc tcgttcgctc gcagagggct cggcacgaag
     8461 gagttggttc cgacccgtgc ttcgaaagag ttcgttccga tgctttggtt tgtgtggttt
     8521 tcggggcccc gggaaccccg aaaacggcgg gccgtcgcat ggttcggaga tccgatgaat
     8581 cctgaggct
//
LOCUS       DASTKS010000217         4424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_702293, whole genome shotgun sequence.
ACCESSION   DASTKS010000217 DASTKS010000000
VERSION     DASTKS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702293"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFQ35_04555"
     CDS             complement(<1..361)
                     /locus_tag="VFQ35_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929931.1"
                     /translation="MKISMLGVLAFVALSGCGSTSDGASSGNDGTLATMGMLGVPSPT
                     LVGQNVMLVDAELNGHAGGRLLVDTGSPLTFVNAASFPGAMLPAQTQVNVDIGIGALT
                     IDDVPSIQTTGGAMDQLH"
     gene            complement(393..3272)
                     /locus_tag="VFQ35_04560"
     CDS             complement(393..3272)
                     /locus_tag="VFQ35_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9929932.1"
                     /translation="MSDALCVVFAEAPVEHGHRERTCRALAAAGQRILDCSRRPLVEA
                     PLSDLRGPLWFVRAGTWPARRFPSAPERFPPPSATGRAVCALGAVVSELGDTPLSAEA
                     AEWSEVLQATGGDFSRALRGIASLPSLASAYVDAELAARVLSDLAAGLALGQSLFAQS
                     SRERLRVLRYAPLDVHSSERLRVAQLVTSLQRGGAERIGLDLASELPQAGVSSRLYAI
                     YSPTRAPFEAPAGAAELAKWLPSSIDRDARFDFLTRELALDGTDLVHAHLLDGRDLER
                     LGARGYRSVVTVHNTSPGFPAGLASVDPAHLALLVGCSRAVEGDLARSGLQAPLRTVW
                     NGIRVESPSSEVGERRRDSPWRAELGLGDDDVLLLALANPRPQKRLHLLPSILAALRC
                     ELSTSRRAAKLVIAGATSPVNELARQAEARLRAEVERLDVTPHVQWAGLVSDVRGLLE
                     ASDVVLSPSEHEGLSLAHLEALSFGVPVIATGSGGTEEIAAEATALRHLELDSSATVW
                     ARAILDVLRMSPLEKKAARDAVARHFNVKRMAANYARLYPRALARTRPAEAGGGIVLV
                     TNNFSTGGAQSSARRLLLGLRSQGVRARAVVIEEQTAYPTPGRRALCDAGVSVLALPP
                     PRELEPGRAVERLLGELERDSPEAVVLWNVIPEYKLLIADGLLDVPLFDVSPGEMYFR
                     SLERYFASTRRPSLPYRTAREYGARLAGVIVKYAAEASRAGQMLGAPVHVIPNGVPLG
                     PSRASAKRRAPGPIVFGTSARIHPDKKLGELLAALRVAAERLPPHVLRIAGAVDGRAE
                     NEAAELRRASVGLNVEWLGEVADTGAFLESLDVFVLIAEPAGCPNASLEAMERGLPVI
                     ATDVGGMSEQIVDGVTGRLIPRGDVEGLALAMLELARRPELAAGFGRAGRERIERQFS
                     LERMVRDYMSVCLRAARNCGHVLERSSKIAAESAHTAVERCAPIR"
     gene            complement(3290..>4424)
                     /locus_tag="VFQ35_04565"
     CDS             complement(3290..>4424)
                     /locus_tag="VFQ35_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9929933.1"
                     /translation="EKGTSGGFGFEGGRLVQRPEFTWKNPGFVQAPTHPVTLVTFGDA
                     LAFTSWLSEKGGRRFSLPTEAEWEYAYRAGTSTPFYQPGDADAALNIGWFKANAANGT
                     HPVAQKTPNVFGLYDMAGNVYEWCRDWYGPYALDATSDPEVSVAPSGDKPRRVLRGGS
                     WLKDAKNLRAAARYRNDPGSRNADNGFRVSASTEVSPAAAEQPKTDSGVGKGTSAAGT
                     DEPSRSTSLVFSAFVALASFALLGLVVAVIRKSRRPRAMRGAPPGVTYRRVKDGFWLV
                     APSPLHGSVLHYRANVVGRAVRASVPIEPAADGQFVYTGGAPVDVEAEQIVGMSAAQA
                     YAAAMAMDARAQRGRPQRGYDPDESESEDVDSSPAFRGYPGAY"
BASE COUNT          781 a         1547 c         1416 g          680 t
ORIGIN      
        1 agtggagctg gtccatggct cccccggtcg tctggatcga gggcacgtcg tcgatggtta
       61 gcgccccgat cccgatgtcg acgttcacct gggtttgcgc cggcagcatt gccccgggga
      121 acgaagccgc gttcacgaac gtgagcggtg agccggtatc cacgaggagc cgtccccccg
      181 cgtgaccgtt caactcggcg tccaccagca tcacgttctg gccgactagc gtcggcgatg
      241 gcacgcccaa catacccatc gtcgcgagcg ttccgtcgtt tccgctcgac gcaccgtcgc
      301 tggtgcttcc gcatcccgaa agcgcgacga acgcgagcac ccctagcatc gatatcttca
      361 tgtgcttcct ccacccacta ggcacctcga ccttatcgga tgggagcgca ccgttccacc
      421 gccgtgtgcg cggactctgc cgcaattttg ctcgaacgtt ccaaaacgtg accgcaattt
      481 cgtgcagctc gcaggcagac gctcatgtaa tcccgtacca tgcgttcgag cgagaactgc
      541 cgctcgatgc gctctcgccc agctcgaccg aagcccgcag cgagttcggg ccgccgcgcc
      601 aattcgagca tcgccagcgc gagcccttcg acgtcgccgc gcggaatcaa gcggcccgta
      661 acgccgtcca cgatctgctc gctcatgccg cccacgtccg tcgcgatcac cggcaacccg
      721 cgctccatcg cctccaacga ggcgttcgga cagcctgcgg gctccgcgat cagaacgaac
      781 acgtcgagcg actcgaggaa cgcgccggtg tcggcgacct cgccgagcca ctcgacgttc
      841 aagccgacgg aggcccggcg cagctcggcc gcctcgttct cggcgcgccc gtcgacggct
      901 ccggcgatgc gcagcacatg aggaggcagc cgctccgccg cgacacgcag agccgccaga
      961 agctcgccga gcttcttgtc ggggtggatg cgcgccgacg tcccgaacac gatgggcccg
     1021 ggtgcgcgac gcttggccga agctctcgac ggcccgagcg gaacaccgtt gggaatcacg
     1081 tgcacgggcg cgccgagcat ctgccccgcg cgcgaagctt cagccgcata cttcacgatg
     1141 acgccagcga ggcgtgcgcc gtactcgcgc gcagtgcgat agggcagact gggcctgcgt
     1201 gtgctcgcga agtagcgctc caacgagcga aagtacatct cgcctgggct gacgtcgaac
     1261 agcggcacgt cgagcaagcc atccgcaatc aagagcttgt actcggggat gacgttccac
     1321 aaaactacgg cttcgggaga gtcgcgttcg agctctccca gcaagcgttc gaccgcgcgc
     1381 cccggctcga gctcacgggg cggcggcagc gcgagaacgc tcactccggc gtcacatagc
     1441 gctcggcgac cgggcgttgg atatgcggtc tgctcttcga tgacgaccgc tcgggcccga
     1501 actccttggg agcgcagccc gaggagcagt cgacgtgcgc tcgattgagc accgcccgtc
     1561 gagaagttgt tcgtaacgag cacgataccg ccgcctgctt cggccggtcg cgtccgagct
     1621 agcgcgcgcg gatagagccg cgcgtagttc gcggccatgc gtttgacatt gaagtgacgc
     1681 gccactgcgt cgcgagcagc ctttttttcg agaggtgaca tccgaaggac gtcgaggatc
     1741 gccctcgccc acaccgtcgc ggagctgtcg agttcgagat ggcggagcgc tgtggcctcg
     1801 gcggcgatct cttccgtgcc gccgcttccg gtggcgatca ctggaactcc aaatgaaagc
     1861 gcctcgaggt gcgccagact gagtccctcg tgctcgctcg gggagagcac gacgtcgctc
     1921 gcctcgagca atccgcgaac gtcgctcacg agccccgccc actgcacgtg aggcgtcacg
     1981 tcaaggcgct cgacctccgc acgcaggcgc gcctcggctt ggcgcgcgag ctcgtttacc
     2041 ggtgatgtgg ccccagctat aacgagcttc gccgctcgcc gcgaggtcga cagctcgcag
     2101 cgcaacgccg cgagaatact cggcaagagg tgcagtcgct tttgcggccg cgggttggcg
     2161 agtgccagca acagaacgtc atcgtcgccg agcccgagct ctgctcgcca gggcgagtcc
     2221 ctgcggcgct cgccaacctc cgagctgggc gactcgacgc gaatgccatt ccaaaccgtg
     2281 cgcagcggcg cctgcagccc cgagcgagcg agatcgcctt caacggctcg tgaacacccg
     2341 acgagcaacg cgaggtgcgc tggatcgacg ctcgcgagcc cggcggggaa tccgggcgag
     2401 gtgttgtgca ccgtcacgac cgaacggtag ccgcgggctc ctagccgctc gagatcgcgt
     2461 ccgtcgagca agtgagcgtg cacgagatcc gtcccgtcga gagccagctc gcgcgtcaaa
     2521 aagtcgaagc gcgcgtctcg atcgatggac gaagggagcc actttgccag ctcagccgcg
     2581 cccgcgggag cctcgaacgg cgcgcgcgtg ggtgaataga tcgcgtacag ccgtgacgag
     2641 accccggcct gcggcagctc cgacgcgagg tcgagcccaa tgcgttccgc gccgccacgc
     2701 tgcagactcg tgaccaactg cgcgacgcga aggcgctcgc tcgagtgcac gtcgaggggc
     2761 gcataacgca gcacccgcaa acgctcgcgg gacgactggg cgaacaggga ctgacccaac
     2821 gcgagccctg cggcgagatc gctcagcacg cgagcggcga gctcggcgtc gacatacgcg
     2881 ctcgcgagcg acggcagcga ggcgatgccc cgcaaggccc gggaaaaatc gccaccggtc
     2941 gcttgcaaaa cttccgacca ttcggcggcc tccgcggaaa gcggcgtgtc acccaattcg
     3001 gatacgaccg cgcccaacgc gcacacggct cggcctgtgg cactcggcgg gggaaagcgc
     3061 tccggcgcgc tcggaaagcg ccgagctggc cacgtgccgg cgcgcacgaa ccagagcggg
     3121 cctcgtaggt cgctgagcgg cgcctcgacg agcgggcgtc gagagcagtc gagtatgcgc
     3181 tgtccggcgg cggcgagcgc gcgacaggtg cgctcccgat gcccatgctc gaccggagcc
     3241 tctgcgaaca cgacacacag tgcgtcgctc atcgccgcct ccccaaccct cagtacgctc
     3301 ccgggtagcc gcggaatgca ggcgatgaat cgacgtcctc cgactcggat tcatccggat
     3361 cgtagccgcg ctgagggcgg ccgcgctgag ctctagcgtc catcgccatc gcagcggcgt
     3421 atgcctgcgc cgcgctcatt ccgacgattt gctccgcctc cacgtcgacg ggggcgccgc
     3481 ccgtgtaaac gaattgacca tctgcagcag gttcgattgg aacggatgcg cgcacggcgc
     3541 gcccgaccac gttggcgcga tagtgaagca ccgagccatg aaggggcgac ggcgcgacca
     3601 gccaaaaacc gtctttcacg cgcctatacg tcacgccggg cggagcccct cgcatcgcgc
     3661 gcggtcggcg cgacttacga atgacggcga cgaccagccc caagagcgca aagctcgcga
     3721 gcgccacgaa cgccgagaag accagcgacg tgcttcggct cggttcgtcc gtgcctgcgg
     3781 cgctggtgcc cttccccacc ccagaatctg tcttcggttg ttccgcggca gctgggctca
     3841 cctcggtcga agcgctcacg cgaaagccat tgtcggcgtt gcggctgccc ggatcgtttc
     3901 gatagcgcgc ggctgcccga aggtttttgg catctttcaa ccaagaaccg ccccggagga
     3961 ctcgacgcgg cttgtctccc gaaggtgcaa cactgacctc gggatcggag gtcgcgtcga
     4021 gcgcgtaagg gccgtaccaa tcgcgacacc actcgtagac attgccggcc atgtcgtaca
     4081 ggccgaaaac gttgggcgtc ttctgcgcaa cgggatgcgt gccgttcgcg gcatttgcct
     4141 tgaaccagcc aatattcagc gcagcgtcag cgtcgcccgg ctgatagaag ggcgtcgacg
     4201 tcccagcgcg gtacgcgtac tcccattcgg cttcggtcgg cagcgaaaac ctgcgccccc
     4261 ctttctctga gagccacgac gtaaaagcga gcgcgtcgcc gaaggtgacc agcgtgacag
     4321 gatgagtcgg cgcttgtacg aagcccggat ttttccaagt gaactccggc cgctgaacga
     4381 gcctcccgcc ctcgaacccg aacccgcccg acgtgccctt ctcc
//
LOCUS       DASTKS010000218         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_926336, whole genome shotgun sequence.
ACCESSION   DASTKS010000218 DASTKS010000000
VERSION     DASTKS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926336"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFQ35_04570"
     CDS             complement(<1..686)
                     /locus_tag="VFQ35_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929934.1"
                     /translation="MSARPAGSQNGPGKWLITPPQTHIPREVIASAAVFRGLAHAAWL
                     IFLARPASATLFAELSVARTESAADCPSASALTERVERIRHATVRPPAAAPTGDVVFV
                     EVGFGRAAGAYSARLAFRGPKRGERELVDSSATCEALAEAVSLAIALALDQSEEPEGS
                     TPSAPATERARPPAARDAAERTGSQEATQPSGHDERLGLGASLESGAAFAFGAPVSLS
                     LGARARGSYRG"
     gene            1323..>2062
                     /locus_tag="VFQ35_04575"
     CDS             1323..>2062
                     /locus_tag="VFQ35_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929935.1"
                     /translation="MTYSPKKLSRSLFGIWSVFAAIGLTHCGSDDSNGGTGGAGGTAG
                     GSAHSGNGGMAGNAGHAGSGADAGAGAGGLGATAGEAGAAGANTGGTGGTAGGTTATG
                     GAGGALGGAANEGGSGGEVTSPFEHFFTGEFVKKVVPARAEKGYFALLEKAGVLHVDY
                     GMPRRRLVKVSGSGEVLPWIATLPWMTPSDDVSLLDFALHPSGEVTALFASSAGYHLV
                     RIAASGELISETALSDTKIALDPPSLPE"
BASE COUNT          361 a          676 c          703 g          322 t
ORIGIN      
        1 cctcgataag aaccgcgcgc tctcgccccg agtgacaacg aaaccggcgc gccgaatgcg
       61 aatgcggcgc cgctctccaa cgacgcacca agcccgagcc gctcgtcgtg gcccgacggt
      121 tgcgtcgctt cctgcgagcc cgtgcgctct gctgcgtcgc gagcagctgg cggtcgggct
      181 cgttcggtcg cgggcgccga tggcgtcgag ccctccggtt cctcggactg atccagcgcg
      241 agcgcaatcg cgagactcac ggcttcggcc aacgcctcgc aggtcgcgct gctgtcgacg
      301 agctcgcgct ctccgcgctt cggtccgcga aaggcgagcc gggccgagta cgcacccgcc
      361 gcgcgcccaa agcccacctc cacgaagact acgtcgccgg tcggtgctgc agccggcgga
      421 cggaccgtcg cgtgccgaat gcgctccacc cgttcggtga gcgcgctcgc gctgggacag
      481 tccgcggctg actcggtgcg tgcgaccgaa agctctgcga acaacgtggc cgacgctggc
      541 cgagccagga agatcagcca cgccgcgtga gctagacctc gaaacaccgc tgccgacgct
      601 atcacctccc gagggatatg ggtttgcggc ggcgtgatga gccatttccc gggcccattc
      661 tgcgaaccgg ctggtcgagc actcatcgcg tccgcgccaa cgggtaggtg cgcgacttag
      721 cagggcttga aaagcgtcct gcgtcacgac agaccaatgc caggcacact attggggccc
      781 gcgaatgaac aagtcagtcg cgagcgggcc gagaccgcag cgaggccggc gcgtgcggag
      841 agagcaccgc aggcctgctg aagcgtcagg atttatcgga tctccgaacc acgcatcggt
      901 ccgccgtttt cggcgtttct ggggccccga aaaccacaca aaccaaagca tcggaacgaa
      961 ctctttcgaa gcacgggtcg gaaccaactc tttcgtgccg agccctctgc gagcgaacga
     1021 gacgaaggcc tacctgctgg cgacatgcga cgaatcctga ggctgatgca ggtcgaggag
     1081 cgcaacgacg agcccgcccg ccgcagcaag gggatcggcc cgcgcagtat gacttgtcaa
     1141 tgcgcgggcc cttagagcac gcgagtgcgc tgcactcggc acggacccga ccgcgatgac
     1201 cacgtgtccg gacgattgtc cgccgagaac gctcgccgtt tcgctgaacc gcgcaagaat
     1261 ggcgcgtggc acgcatcgtg aatctggctc attcctttcg cttgctcctt cgagaggtct
     1321 gcatgactta ttcgcccaag aagctgtcac ggagtctgtt cgggatctgg tccgtcttcg
     1381 ctgccattgg cttgacccac tgcggctcgg acgattcgaa cggggggact ggaggcgccg
     1441 gtggaaccgc gggcggaagc gcgcacagcg ggaatggcgg catggccggc aatgcgggcc
     1501 acgcaggaag tggtgcagat gcgggcgcag gcgcaggcgg gcttggcgct accgctggcg
     1561 aggccggcgc ggccggagcg aatacgggag gcaccggcgg aacggcgggc ggcacgacgg
     1621 ccacgggggg cgcgggtggt gcgctcggcg gtgccgcgaa cgagggcggc tcgggcggcg
     1681 aagtcacgtc gccattcgaa cacttcttca ccggcgagtt cgtcaagaag gtggtgcccg
     1741 ctcgagctga aaagggctac ttcgcgctgc tcgagaaagc gggcgtgctg cacgtagact
     1801 atgggatgcc gcgacgacgt ctcgtcaaag tgagcggcag tggcgaggtg ctgccttgga
     1861 tcgcaaccct cccctggatg acgccgagcg acgacgtctc gctgctcgat tttgctcttc
     1921 acccatccgg agaggtgacg gcgctgttcg cttcgtccgc gggctatcac ctggtgcgca
     1981 tcgctgcgag cggcgagctc atcagcgaga ccgcgctttc ggacacgaag atcgcgctcg
     2041 atcctccgag cttgcccgag ga
//
LOCUS       DASTKS010000219         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_702998, whole genome shotgun sequence.
ACCESSION   DASTKS010000219 DASTKS010000000
VERSION     DASTKS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702998"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFQ35_04580"
     CDS             complement(<1..686)
                     /locus_tag="VFQ35_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET9929936.1"
                     /translation="MPAVKVPAPVIGRLAPSPTGLLHLGHARTFLVAWWSARSQHGRV
                     VLRVEDLDGARSEQKYIDAALRDLEWLGLDWDGAPLIQSSGLPRLSAAIDGLVARGLA
                     YPCTCTRGDIRNAQSAPQQGVTEPRYPGTCRGRYASLAEAEAQSGRPAGIRLRVPEGY
                     VEFVDRLAGPQRFDVQAEVGDFLIARRNGAPAYQLAVVVDDAHQSVNEVVRGNDLLSS
                     TARQIVLERAL"
     gene            complement(674..1291)
                     /locus_tag="VFQ35_04585"
     CDS             complement(674..1291)
                     /locus_tag="VFQ35_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929937.1"
                     /translation="MKSWGARSGIAAGLLTLAFVGCATSSETRSAPEPAPQSAAPVVA
                     PAPPPAKPEAPKSADKADDAGTDFATLDEAEAALERARTDLNRLALLDRPGGFAEAPA
                     AAAPPAPSRAGRSAGAARDEAPREKKATRPHEAKAAPAESEAAEAAAAKGENPCDTGC
                     KAFASLMRAKAAVCRLDSPGGARCSRAEDIAREAEGRVRSCGCQQ"
     gene            complement(1405..2229)
                     /locus_tag="VFQ35_04590"
     CDS             complement(1405..2229)
                     /locus_tag="VFQ35_04590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9929938.1"
                     /translation="MPPRRPRSPQSKPRFTAKTADKHDLYQRSVQDADAEAAFIERVY
                     KQLRGRAPQSLREDFCGTALLTATWVKRGPDRRGIGVDLDPSVLAWGREHNIAPLGEA
                     TERVELYCQDVRDKVAEKVDVVGAFNFSYWIFFTREEMKNYFSLVRRGLNKDGVFLLD
                     AYGGWESQEPMLEERAIPGGFTYVWDQDQFDPITHRIRNYIHFHFKDGTKLEKAFTYE
                     WRYWTLPELTELLGEAGYSDVRVYWDTSDDDEHEAYKPRKRADNQPGWLAYITATV"
     gene            complement(2261..3577)
                     /locus_tag="VFQ35_04595"
     CDS             complement(2261..3577)
                     /locus_tag="VFQ35_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929939.1"
                     /translation="MLARSLVQTAFATLALQAVACHEKSATSSATPSAKPLIPSTAAP
                     SAAPRTSAPAAAPLEAKTNPAPPAGSATPVAPGTGPFVIDEAFDVGPAGPAAAHESGV
                     VLVDKLGHVALAKRGPLPTGSVASKGSFSAVDRPRDEFLPYARGPALVGGFAYWIKDE
                     KLVRARVTGNDADPEEIASDARKGARVSGAEMPDGTPVVAFITKPDSSGAPHAKVWFR
                     GKVFDLTPEGAGASSVALVRSGPRIMAISLDGRSGMTPLHARLLSVEQSGALALGPDV
                     VAWVGASAQATTEVFATAAEGDVWAFVPIEQDLTHFGLAQIHLGSEPRLDAKVTFLTY
                     QAGTNSAPVAAGIACGRSAVAFAEPEEAAPKSPQRLVVAAVGAEGLEPGEVVARGRSF
                     ANVSLGAAGPGALLVYTADHRTWARSVRCRDRARPHSELPGALEKR"
     gene            3705..4055
                     /locus_tag="VFQ35_04600"
     CDS             3705..4055
                     /locus_tag="VFQ35_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929940.1"
                     /translation="MRRSAGFLLALWVGALSACAPTRVAAAPGSHDRPVPADEPRAQL
                     KLELDLPKTASCEEDFDLRLYANPAVDLIEWRGNGQKCSGRRVTISYLSRRTDADKVL
                     MQVKGLAANARVMP"
     gene            4129..6228
                     /locus_tag="VFQ35_04605"
     CDS             4129..6228
                     /locus_tag="VFQ35_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9929941.1"
                     /translation="MISTKKSRWFLAAVSLLAILPSLGVFTRAFAQEEGEAEVEDGEA
                     GVGAGLGKRQRAILEALTKDSNALVNVSFADMPASPDILNLGRRGTEALARCVADNVD
                     DGVRSTCAMVLGRLGDRRGLSALQEALEAWAPEVRAAAITALARMPDPSSYPALAALF
                     AREDETPENRLAVLNALGMLSDARAVQLLRKVLHDPNESGLHAPAFQAAWKSRHLFAR
                     PTLVADVKYALGGNDYQLILSATYAAAELRDPALVPALVPLMNNSDSHIRNRAVFALG
                     RIGDRAASSALVAQIPKVREARMLNNIAFALERLDPKAFFAAIDRLAQHKQASIRMNA
                     AFVLGDVRRPEGLPLLARALKDQNDFVRVSAVAALGKLDAPEAIPLVSPFVSDKNRTL
                     KKTAIFSLLSLSNGQKRDVVYDALVAVPPGDAFTEQSRMEGVLALAELGDPRVLDPVV
                     ECVEQRRCSRGTVEPFLLQHRSPALGGRMLLAWAKGRSDLTDLVAALRPTGAGLLAAS
                     EVRALGPNGFWSRLESAVDLAGAVGEKQAVDVLTSFAKHQNTALRMHASVALARLAQP
                     NADAAVFGDFDNLPSSWLPRFSRLLSSVDDATVRARWQPTLLQREQGTDRSLALASAA
                     VRMAWWPDPGVFRFLDGLASSSAEERELTMRYLQRDQRSVTTSLLRRAYARETRPFVR
                     DLLRKLLDSRRGAPGVP"
     gene            6225..>7079
                     /locus_tag="VFQ35_04610"
     CDS             6225..>7079
                     /locus_tag="VFQ35_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929942.1"
                     /translation="MSPRLERLLPWALALVCLIFDPSALADAPKRTLALLLSLGLLVR
                     ALGRGEALRLPRATSCLLVIVGLVGVSALYGSGARWLLLGNWWLIAALAVVLGELELE
                     VARGVMARTALLVSLGTSAWLLASWLCGARGMLLHAGQGNPNWAGLVLAIAWPLSLRD
                     ASFARARSVLVLGYGVACATALALSESRVAWLAVGVAWVGLTFRSTSRRHAFAQLALL
                     VGAISLTALRSPIGASSARPATNVSAAHPASHAATRAAPELDHSARTSLSGRIWIQRV
                     ALRQAIARL"
BASE COUNT         1048 a         2365 c         2422 g         1244 t
ORIGIN      
        1 agcgcgcgct cgaggactat ctgccgagcc gtactcgaca acagatcgtt gccgcgcacg
       61 acctcgttga cgctttgatg ggcgtcatcg accacgaccg cgagctggta ggcgggagcg
      121 ccgtttcggc gcgcgatgag aaagtcgccc acttctgcct gaacgtcgaa gcgctgcggc
      181 cctgccaagc gatcgacgaa ctcgacgtac ccttctggca cgcgcaggcg aatgccagca
      241 gggcgccccg attgggcttc ggcctccgcg agcgaagcgt agcgcccgcg gcaggtcccc
      301 ggatagcgcg gctccgtcac cccctgctgc ggcgcactct gcgcgttgcg aatgtcgccg
      361 cgcgtgcagg tgcaagggta ggcgagccca cgcgcgacga gcccgtcgat ggccgctgaa
      421 agccggggga gcccgctcga ttggatgagc ggcgcgccat cccaatcgag ccctagccac
      481 tccagatcac gcaacgccgc gtcgatgtac ttctgctccg aacgagcgcc gtcgagatct
      541 tcgacacgca gcacgacgcg gccatgttgc gagcgcgccg accaccacgc caccaagaac
      601 gtgcgagcgt gcccgagatg caagagcccc gtcgggctcg gcgccaaacg accgatgacg
      661 ggggcgggga cctttactgc tggcatccgc agctgcgcac gcgtccctcg gcctcgcgcg
      721 cgatgtcttc ggcgcggctg cagcgcgcgc cgccgggcga atccaagcga cacactgccg
      781 ctttcgcgcg catcagggat gcgaatgcct tgcaaccggt atcgcagggg ttctcgccct
      841 tggcggctgc tgcctccgca gcttcgcttt cggccggcgc cgccttggct tcgtgggggc
      901 gtgtcgcttt cttctcccgg ggcgcctcgt cccgtgcggc gccggcgcta cgtcctgcgc
      961 ggctgggtgc tggcggagcc gccgcggcgg gcgcttcggc aaaaccgccg gggcgatcga
     1021 gcagggccag ccgattcaaa tcggtgcgcg ctcgttcgag cgcggcctcg gcttcgtcga
     1081 gcgtcgcgaa gtcggtgcct gcgtcgtccg ctttgtcggc cgacttgggc gcctcgggct
     1141 tggcgggcgg cggcgccggc gcgacgacgg gagcggcact ctgcggcgcc ggttcagggg
     1201 cgctgcgcgt ttcgctcgaa gtagcgcacc cgacgaatgc cagggtgagc agcccggctg
     1261 cgattccgct ccgcgctccc cacgatttca ttgcgattga cccaggcctg cgcaacttcg
     1321 ccatctcgct cttagacgca cgagcctacc ctgtggctca atgctttggt gatttttccg
     1381 agctcgggcg ggctcttgcc gcgctcagac ggtcgccgtg atgtaggcga gccagcccgg
     1441 ctgattgtcg gcgcgcttac ggggcttgta ggcctcgtgc tcgtcatcgt cgctggtgtc
     1501 ccaatatacg cgcacgtcgg aataacctgc ctcgccgagc aactcggtga gctccggcag
     1561 cgtccagtag cgccactcgt aggtaaacgc cttctcgagc ttggtgccgt ctttgaagtg
     1621 aaagtgaatg tagttacgga tgcgatgcgt aatggggtcg aattgatctt ggtcccagac
     1681 gtaggtgaag ccgcccggga tggcgcgctc ttcgagcatg ggctcctgcg actcccagcc
     1741 gccataggcg tcgagcagaa agacgccgtc cttgttcaga ccccgacgca cgagcgagaa
     1801 gtagttcttc atctcctcgc gtgtgaagaa gatccagtaa ctgaagttga aagcgccgac
     1861 cacgtcgacc ttctcggcca ccttgtcgcg cacgtcttgg cagtagagct cgacgcgctc
     1921 ggtagcctcg ccgagcggcg cgatgttgtg ctcgcgaccc caagcgagca cgctcggatc
     1981 gagatcgact ccgattccac ggcggtccgg ccctcgcttg acccaggtcg cagtgagcaa
     2041 agcggtgccg cagaagtcct cgcgcaggct ttgcggagcg cgtccgcgga gctgcttgta
     2101 gacgcgttcg atgaaagccg cctcggcatc ggcgtcttgc accgagcgct ggtagagatc
     2161 gtgcttgtcg gcggtctttg ccgtgaagcg cggcttggat tgaggcgagc gggggcgacg
     2221 gggtggcatc ggttttcttg atcctcgaga ggcgccgctt ttagcgcttt tcgagggcgc
     2281 cgggcagctc cgaatgtggc cgcgctcgat ctctgcagcg tacgctgcgc gcccaggttc
     2341 ggtggtcggc cgtatagacg agcaaggcgc cggggccggc ggctccgagg ctcacgttgg
     2401 cgaacgagcg cccgcgtgcg acgacctccc cgggttcgag cccctcagct ccaaccgccg
     2461 cgaccacgag gcgttgcggc gactttggcg ccgcctcttc gggttcggcg aaggcgaccg
     2521 cgctgcgtcc acacgcgatg cccgcggcga ccggggccga gttcgtcccg gcttgatacg
     2581 tgagaaaggt cactttggcg tcgagccgcg gttcgctccc gaggtgaatt tgcgcgagcc
     2641 cgaagtgcgt caggtcttgt tcgatcggaa cgaacgccca cacgtctccc tccgccgcgg
     2701 tcgcgaatac ctcggtggtc gcttgcgccg aagccccgac ccaagcgacg acgtcgggac
     2761 ccaacgcgag cgctccggac tgctcgacgc tgaggagacg agcgtgcaaa ggcgtcatgc
     2821 cgctccgccc atcgagcgaa atcgccatga tccgagggcc cgagcggacg agtgcgacgc
     2881 tgctcgcgcc ggcaccttcg ggcgtcaaat cgaacacttt gccgcgaaac caaacctttg
     2941 cgtggggcgc gccgctcgag tcgggcttcg tgatgaaggc gacgacgggc gtaccgtctg
     3001 gcatttcagc tcccgatacc cgagcgccct tgcgagcgtc gctcgctatc tcctccgggt
     3061 cggcgtcgtt cccggtcacg cgcgctcgta ccaatttctc gtccttgatc cagtaggcaa
     3121 agccgccgac cagggccggt ccgcgcgcgt agggcaagaa ttcgtcgcgc gggcggtcga
     3181 ccgcgctgaa gctccctttg gaagcgacgc ttccggtcgg cagcggcccg cgtttggcga
     3241 gagcgacgtg tccgagcttg tcgaccagga ccacgccgct ttcgtgagcc gcggcaggtc
     3301 ccgcaggacc tacgtcgaaa gcctcgtcga tgacgaacgg ccccgttccc ggggccaccg
     3361 gagtcgccga gccggcaggc ggtgcagggt tcgttttggc ttccagcggg gcagcggcgg
     3421 gtgccgacgt tcgcggcgct gcgctcggag cggcggtgct cgggattaag ggctttgccg
     3481 acggcgttgc agagctggtg gcggactttt cgtggcaagc gactgcttgg agtgcgagcg
     3541 tggcgaaggc agtctggacg agcgagcgag cgagcattgg cgagtatctt agacccgagg
     3601 ccgttccgtc gacgcgagtt tccgcgagtt cgggagcgaa ggtttggagc gctccgtgcc
     3661 ggccccgctt ctggctgcgg aatgggcgtg ctaacggacg aggtgtgaga cgctctgcag
     3721 ggtttctatt ggcactttgg gttggtgcgc tgagcgcttg tgcgcccacg cgcgtcgcgg
     3781 cggctccggg cagccacgat cggcccgtac cggcagacga gccgcgagcc caattgaagc
     3841 tcgagctcga cctgcccaag accgcgagct gtgaagagga cttcgacttg aggctctacg
     3901 cgaaccccgc ggtggatctc atcgagtgga gaggcaacgg gcagaagtgc agcggtcgcc
     3961 gcgtgacgat tagctatctg tcgcgccgca ccgacgcgga taaagtgctc atgcaggtga
     4021 aaggtttggc ggcgaacgcg cgggtgatgc catgagccgc ctcattgctc gtcgagttac
     4081 gcgagggctt gcgccgagct ttcgcggcga aggcggaggg tgcctccaat gatcagcacg
     4141 aagaagtcgc gatggttctt ggcggccgtg tcactactcg cgatcctgcc gagcctcggc
     4201 gtcttcacgc gcgctttcgc gcaggaagaa ggcgaggcgg aggtagagga cggtgaagcg
     4261 ggagtcggcg ctgggctcgg caagcgacag cgagccatct tggaggcgct caccaaagac
     4321 tcgaacgcgc tggtcaacgt atcgttcgcg gatatgcccg cgagccccga catcttgaac
     4381 ctgggccgcc gcggcacgga ggcactcgca cgctgcgtcg cggacaacgt cgatgatggc
     4441 gtccgctcca cctgcgcgat ggtgctcggg cggctcggcg atcggcgcgg tctttcggca
     4501 ctgcaagagg cgctcgaggc gtgggccccc gaggtgcgcg cggccgcgat cacggcgctc
     4561 gcccgcatgc ccgacccgag ctcgtatccg gcgctcgcgg cgctctttgc gcgtgaggac
     4621 gaaacgcccg agaaccgact cgccgtgctg aacgcgctcg gcatgctctc cgacgcgcgt
     4681 gccgttcaac ttttgcgcaa agtgttgcac gacccgaacg agtctgggct gcacgccccg
     4741 gcgttccagg cggcatggaa gagccggcac ttgttcgcgc gaccgacgct ggtcgccgat
     4801 gtcaagtacg ctctcggggg caacgactat caactgatct tgtccgcgac gtacgcggcc
     4861 gcggagctgc gcgatccggc cttggtgccc gcgctcgtgc cgctcatgaa caactccgac
     4921 tcgcacatcc gcaaccgtgc cgtcttcgcg ctcggtcgca tcggggaccg ggccgcgagc
     4981 agcgcgctcg tcgcacagat cccgaaggtg cgcgaggccc gcatgctgaa caacatcgcc
     5041 ttcgcgctcg agcggctcga cccgaaggcg ttcttcgcgg caatcgatcg gctcgcgcag
     5101 cacaagcaag cgtcgattcg catgaacgcc gccttcgtgc tcggcgacgt gcgtcgtcca
     5161 gagggtttgc cgctattggc acgcgcgctc aaagaccaaa acgacttcgt gcgcgtgagc
     5221 gcagtggccg cgctcggcaa gctcgacgcc cccgaggcca tccccctcgt cagccctttc
     5281 gtgagcgaca agaaccggac gctgaagaag acggcgatct tctcgttgct ctcgttgagc
     5341 aacgggcaga aacgcgacgt cgtctacgac gctctcgtcg cggtcccgcc tggcgatgcg
     5401 ttcacggaac aatcccggat ggaaggggtg ctcgccctcg ctgagctcgg agacccccgc
     5461 gtcttggatc cggtggtcga gtgcgtcgag cagcgccgtt gctctcgcgg cacggtcgag
     5521 cctttcctgc tccagcatcg aagcccggcg ctcggcgggc gcatgctgct cgcgtgggcc
     5581 aaaggtcgca gcgacctgac cgacctcgtc gcggcgttga gaccaaccgg cgctggcctg
     5641 ctcgccgcga gtgaggtgcg cgcgctcggg ccgaacggat tttggagccg cctcgagagc
     5701 gccgtggatc tcgccggcgc cgtcggtgaa aaacaagcgg tcgacgtgct gacgtcgttc
     5761 gccaagcacc agaacaccgc gcttcgcatg cacgcgagcg tcgcgctggc tcgcctggcg
     5821 cagccgaacg ccgacgcggc cgtgttcggc gatttcgaca acctgccgag ctcgtggctg
     5881 ccccgcttct cgcggctcct atccagcgtc gatgacgcaa ccgtacgcgc gcgctggcaa
     5941 cctacgttgt tgcagcgtga gcaaggcacc gatcggtcgc tcgcgctcgc ctccgccgcc
     6001 gtccgcatgg cgtggtggcc ggacccgggc gtgtttcgct tcttggacgg tcttgcctcg
     6061 agtagcgcgg aggagcgcga gctcaccatg cgctatttgc agcgagatca gcggtcggtg
     6121 acgacgagct tgctgcggcg cgcctacgct cgtgagacgc gcccgttcgt gcgggacctg
     6181 ctgcgcaagc tgctcgactc tcggcgcggc gcgcccggcg ttccgtgagc ccgcgtctcg
     6241 agcgcctttt gccgtgggcg ctcgcgctgg tctgtctgat cttcgatccc agcgcgctcg
     6301 cggatgcgcc caaacgcacg cttgcgctgc tgctgagcct cggactgctc gtgcgcgcgc
     6361 tcggccgagg cgaagcgctg cgattgcctc gagcaacgag ctgcctcttg gtcatcgtcg
     6421 gcctcgtcgg tgtgagcgcc ctgtacggct ccggcgcacg ctggcttttg ctcggcaact
     6481 ggtggctcat cgcggcgctc gcggtcgtcc tcggtgaact cgaactcgag gtggcgcgcg
     6541 gggtcatggc tcggaccgcg ctcttggtgt cgctcggaac ctccgcctgg ctgctcgcta
     6601 gctggctctg cggagcgcgg ggaatgctcc tgcacgcagg gcagggaaac ccgaactggg
     6661 cgggcctcgt gctcgcgatc gcgtggccgc tctcactgcg cgatgcttcg ttcgcgcgtg
     6721 cgcggagcgt gctcgtcttg ggatatggcg tcgcctgcgc cacggcgctc gccctcagtg
     6781 agtcgcgcgt tgcctggctc gccgttggcg tcgcctgggt tggcctcacg ttccggagca
     6841 cttcgcggcg acacgccttc gcgcagctcg cgctgctcgt gggagccatc agcctgacgg
     6901 cgcttcgctc accaatcggg gcatcgagcg cgcgccctgc gacgaacgta tcggcggctc
     6961 accctgcttc gcacgcagcg acccgcgccg cgccggagct cgaccattcg gcgcgcacct
     7021 cactgtcggg gcggatttgg attcagcggg tggcacttcg acaggcaata gcgcgcttg
//
LOCUS       DASTKS010000220         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_481598, whole genome shotgun sequence.
ACCESSION   DASTKS010000220 DASTKS010000000
VERSION     DASTKS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481598"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..608)
                     /locus_tag="VFQ35_04615"
     CDS             complement(120..608)
                     /locus_tag="VFQ35_04615"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET9929943.1"
                     /translation="MPSFDVVSKVDWAEFQNALQQAQREIAQRFDFRGTETSIERNEN
                     SLTILANAEDRARAALSVLEEKLIRRKVSLKHLDPKPPERGPKGSTRLPVGVKEGIEQ
                     DKAREIVKLIKDAKLKVQASIHEDSVRVSGKKRDDLQEVIALLRGAESLGLDLQFNNF
                     RD"
     gene            complement(658..1890)
                     /locus_tag="VFQ35_04620"
     CDS             complement(658..1890)
                     /locus_tag="VFQ35_04620"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET9929944.1"
                     /translation="MTIDLRSTLERLYALIPQGKRLGLERMQAACARFGNPERSFTSV
                     HVAGTNGKGTVCAFVSSMARSAGLRVGTYTSPHLHRFAERIAIDGAPIEDDLLVSVLN
                     RVMDAATDLTFFEVATLAAFLAFKEAGVQLAVLEVGLGGRLDATNVIPPPRVAAITRV
                     AFDHMAELGDTLGKIAFEKAGIIKPGSAVVLGKLHPEARAVIEPRVAEVGARLVALGS
                     PEPIAGAPLAYPRIALLGSNLAVAVTIGRELGWAPEVLAQGVENTVWPGRNELLHRNG
                     QDLTLLDCAHNPDGAVALSHALDPTVLAEVESRREIALVFGALSTKNWRAMLRRLEQV
                     AGQRIYVAAPISKAVDPREMLAVSNGEVAASVEQALTRARELVGPKGVVVVAGSTFLV
                     GAARALLLNLPTDPPVAL"
     gene            complement(1887..2735)
                     /gene="accD"
                     /locus_tag="VFQ35_04625"
     CDS             complement(1887..2735)
                     /gene="accD"
                     /locus_tag="VFQ35_04625"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET9929945.1"
                     /translation="MTLPERTPPQSTSAEKRSVGKGLFTKCAGCGAVSTAEELNAKWH
                     VCSACGHHHRMSAEQWRRLLLDDGRLEVWDDHIATTDPLKFEDGKPYPERVQRARRSA
                     STSEAVETGSARVEGLRIAYGSFIFSFMGGSMGSVVGERITRLFERATSERLPVVLLH
                     SSGGARMQEGILSLMQMAKTVSALRRYREVRRPFLSVLLDPTTGGVAASTAFLGDVNL
                     VEPNALVGFAGPRVIESTLRTTLPEGFQRAEFLLSHGMVDAIVSRMEMKATIARLIRH
                     LGGGIT"
     gene            3059..>3647
                     /locus_tag="VFQ35_04630"
     CDS             3059..>3647
                     /locus_tag="VFQ35_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9929946.1"
                     /translation="MSSAEAQLAGIPLIGRDDELAQLRRAYYEVLEGGGARIVTLVGP
                     TGIGKRRLARDLIAEVRRAAPATRVYEGRPGPQTTAYGMFARLLRSRFGLTEHMDSDT
                     ARTTLHSQVSAVLGDRRVGDVCYFLGELMGLAFAESPMTRAVAEDPSQARHLRRAVVR
                     SFVEADAAKSPVCLVLDGLHATDEDSVELLGYLVEH"
BASE COUNT          601 a         1188 c         1214 g          644 t
ORIGIN      
        1 ccggtgtcat gggggccgtg ttggcgcgcc cgcgaatgcc gtccgtcccg aatagctttc
       61 tttcagtcat tggtgtctca gtgctgtgtt tcggttcgct cgcggcgctc ggcccgagat
      121 caatcccgga agttgttgaa ctgtaggtcg aggcccaggc tctcggcgcc gcgcaagagc
      181 gcgatcacct cttggaggtc gtcgcgcttt ttcccgctca cgcgcacgct gtcttcgtga
      241 atcgatgcct gcaccttgag cttcgcgtct ttgatgagct tgacgatctc tcgagccttg
      301 tcctgctcga tgccctcctt aacgccgacg ggtaggcgcg tcgagccttt aggtccgcgc
      361 tcgggaggct tcggatcgag gtgcttcagg ctgactttgc ggcgaatgag cttttcttcg
      421 aggacgctca gcgcggcgcg ggctcgatct tccgcgttcg cgaggatggt cagagagttc
      481 tcgttccgct cgatgctggt ttcggtgccg cgaaaatcga agcgctgcgc gatctctcgc
      541 tgggcttgtt gcagggcatt ttgaaactct gcccagtcga ctttcgagac gacatcgaac
      601 gaaggcatag gaactccttt gcgaagcggc gcgcccctcc gacccgagcg cgacccttca
      661 gagcgccacg ggcggatcgg tcggtaagtt caggagcagc gcgcgagccg ctcctaccaa
      721 gaaggtcgaa cccgcgacga cgacgacgcc cttcggcccg accagctccc gagctcgcgt
      781 gagcgcttgt tcgacgctcg cggcgacctc cccgttcgag accgccagca tctcgcgcgg
      841 atccaccgcc ttcgagatcg gcgccgccac gtagatccgt tggcctgcga cctgctcgag
      901 tcgccgcagc atggcgcgcc aatttttcgt actcagcgcg ccgaagacga gcgcgatttc
      961 gcggcgcgat tcgacctccg cgagcaccgt cggatcgagc gcgtgcgaca gcgcgaccgc
     1021 gccgtcagga ttgtgcgcac agtcgagcag cgtgagatct tgcccgtttc ggtgcaggag
     1081 ctcgttccgc cccggccaca ccgtgttttc gacgccctgc gcgagcacct cgggtgccca
     1141 gccgagctcg cgaccgatcg tcacggcaac ggcgaggttc gaccccaaga gcgcgatgcg
     1201 tggataggcg aggggcgcac ccgcgatcgg ctcggggctg ccgagcgcaa ccagccgagc
     1261 cccaacctcg gcgacccgcg gctcgatcac ggcgcgcgcc tcggggtgga gcttgccgag
     1321 cacgacggcc gagcccggct tgatgatgcc ggccttctcg aaggcgatct tgccgagggt
     1381 gtccccgagc tccgccatat ggtcgaaggc gacgcgggtg atcgcggcca cgcgcggcgg
     1441 cgggatcacg ttggtcgcat cgaggcgacc gccgagccca acctccaaaa ccgccagctg
     1501 aaccccggcc tctttgaaag ccaagaaggc ggcgagcgtc gcgacctcga aaaaggtgag
     1561 atccgtcgcc gcgtccatca cgcggttgag caccgaaacc agcaggtcgt cttcgatcgg
     1621 agcgccgtct atggcgatgc gttcggcgaa gcgatgcagg tgcggcgagg tgtacgtacc
     1681 gacgcgcaaa ccggcgctgc gggccatcga cgatacgaac gcgcagacgg tgcccttgcc
     1741 gttggtgccg gcgacgtgga ccgaggtgaa cgaacgctcc ggatttccga agcgcgcaca
     1801 cgcggcctgc attcgctcca aaccaaggcg cttgccctgc gggatgagcg cgtaaagccg
     1861 ctcgagcgtg gacctgagat cgatcgtcac gtaatgccgc cccctaaatg ccggattaga
     1921 cgggcgatgg ttgccttcat ttccatgcgg gaaacgatgg catcgaccat gccgtgcgag
     1981 agaagaaact ctgcgcgctg aaagccctcg ggcaacgtcg tgcgcaaggt gctctcgatc
     2041 acgcgcggac ctgcgaaccc gacgagcgcg ttgggctcga ccaggttcac gtcgccgaga
     2101 aacgcggtgc tcgccgcgac gccaccagtg gtcgggtcga gcagcacgct caagaaaggt
     2161 cgacgcacct cacggtaacg cctgagcgcc gaaacggtct tcgccatttg catgaggctc
     2221 aagatgccct cctgcatgcg tgcaccgccg gaagaatgca gcaacaccac tggcagtcgc
     2281 tcggaggtgg ctcgctcgaa gagccgcgtg atgcgctcgc cgacgaccga gcccatgctt
     2341 ccccccatga aggagaagat gaaacttcca taagcgatcc gcaggccctc gacacgagcg
     2401 ctgccggtct cgacggcctc gctcgtcgac gcgctccgcc gagcccgctg aacgcgctcg
     2461 gggtaaggct taccgtcctc gaatttcagc gggtccgtcg tcgcgatgtg gtcatcccac
     2521 acctcgaggc gaccgtcgtc gagcagtagt cgccgccact gctcggcgct cattcgatgg
     2581 tgatggccgc aagcgctgca tacgtgccac ttggcgttca gctcttccgc ggtcgacacg
     2641 gcgccgcaac cggcgcactt ggtgaatagc cccttgccga cggaacgctt ctcagcgctg
     2701 gtcgactggg gtggagttct ttcaggcaga gtcacgaggg agcagagggt agcaaaaccc
     2761 gtgtgactcc tctaatagat cgcctcgtcc ggctctcgcg ccgcatttct accgcgcggc
     2821 gcaccgcggc aatcagggtg atgcctgcgt cgagccgagc ctgaaggcga ggcgctgagg
     2881 agggtcttgc cgtcggtttg cgccccgggc gggccgactt ggctcgcgtc cgggtgagca
     2941 gatgcgcttg gtcgagaatc gggctggccg accgatgttt cggctcttgc tcggaaacat
     3001 tgcactgagc tggggttcgg cttgaggcgg cgttggctcc cgagacatta tgcgcgccgt
     3061 gagttcggct gaggcccagc tggccggcat tcccctgatc gggcgcgatg atgagctcgc
     3121 ccagcttcgg cgagcctact acgaggtgct cgagggggga ggcgcgcgca tcgtcacgtt
     3181 ggtcgggccg acaggcatcg gaaagcgtcg gctggcccgc gacctcatcg cggaggttcg
     3241 gcgcgcggca cctgccacac gcgtgtacga gggacgaccg ggcccgcaga cgacggcgta
     3301 cgggatgttc gcgcgactct tgcggtcgcg ctttggcctc accgagcaca tggattcgga
     3361 cacggcgcgg acgacgttgc actcgcaggt gagcgcggtg ctcggcgacc gacgcgtcgg
     3421 cgacgtttgt tacttcctcg gtgagctcat gggcctcgcg ttcgcggaga gccccatgac
     3481 gcgcgcggtc gccgaggatc cgtcccaagc gcgacatctg aggcgcgcgg tggtgcgaag
     3541 cttcgtcgaa gccgacgccg cgaagagccc ggtgtgcctc gtgctcgatg ggctgcacgc
     3601 gacggacgag gattcggtcg agctgctcgg ctatttggtg gagcacc
//
LOCUS       DASTKS010000221         5072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1596541, whole genome shotgun sequence.
ACCESSION   DASTKS010000221 DASTKS010000000
VERSION     DASTKS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596541"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            686..1603
                     /locus_tag="VFQ35_04635"
     CDS             686..1603
                     /locus_tag="VFQ35_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HET9929947.1"
                     /translation="MTCASPWACPVCACNVYASRAAEITKAIERWNETPGHAVSLLTL
                     TVRHALGDDLREVRRVLTDAFRALWRGQSGGRLRRELGVTHFVRSLEVTHGANGWHPH
                     LHVLLFQSRKPAADAVDKLKTRWRKIFEHQARRLRTRALGLTGELRAEAERAAATLEH
                     ATPSIEHGADLRLSRNARYIEKLGLEIASIATKRSQARTSLTPWEIAWRAADGDRRAQ
                     TIWTAYTEAMHGARQLTWSRGARAALGLSMSDEDERHAGEHHGELVASVPAAVWDRCS
                     RVAGWTIELLRRARGERAGEECAVLIKAS"
     gene            1817..2044
                     /locus_tag="VFQ35_04640"
     CDS             1817..2044
                     /locus_tag="VFQ35_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929948.1"
                     /translation="MRRSRESPPQKHHTPPPKEPRAPKTVAALVAPEPTVEKKRKPRV
                     HKVTADDILKGAKARTLLRRTCGEATRKAIF"
     gene            2058..2798
                     /locus_tag="VFQ35_04645"
     CDS             2058..2798
                     /locus_tag="VFQ35_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929949.1"
                     /translation="MASETTSLSIARAAKFLNVSRSRVNQFVASGQLTKSGPPGRQTI
                     PLVELRELLDARTAEAAGDESEASATRFERELRVQREKELADWERRQALDLEEAVFDE
                     RKRATREREQLERDALHEMKKHTKVLERVAKSLDSLQLVLGGALAAALVPNEKKAEAW
                     ANLFGESQPRKQAESPEPVRERLGELSALVARGSGEPLRAVPKDEHPGPPLPTAEEVK
                     DPANWEKVFSTWREILEARSRGKQGVTS"
     gene            2795..3601
                     /locus_tag="VFQ35_04650"
     CDS             2795..3601
                     /locus_tag="VFQ35_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929950.1"
                     /translation="MRFSGEEFGLVDTTFERKVAEMEPAHPPLSVVDSQPKKGPWTGN
                     NNLGYRLKFAPDADNRQTILKLPEWGFPEVWTVSLALSGFPGGLFDGFGVTGHLEFGV
                     GGATENIAVDWANGTQVSLAMNALNVIAEFSNLDVTEEGQGLELIVQLARGNRPGGSR
                     PPTLTMLRDEPFGAGADTGNLEIPAFASRIIAVPSDPTVAGVTNFYSSDIVMDLVTGN
                     VGSGHVVSAVSGAQLASVRGLDVVGDARFARLINHGTNATRVTVYAELAA"
     gene            3601..3909
                     /locus_tag="VFQ35_04655"
     CDS             3601..3909
                     /locus_tag="VFQ35_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929951.1"
                     /translation="MARVFAKRVPYSYTRAAPHVPCAGSAPVVLPRDSGPVEAVEHTE
                     APSPVPRPAERPCAGTVATALAPDSPPLESRPKKPRWEPEQRPRHSHVPCAGTVATKL
                     "
     gene            3923..4633
                     /locus_tag="VFQ35_04660"
     CDS             3923..4633
                     /locus_tag="VFQ35_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929952.1"
                     /translation="MSSVNVSYPCAGTVPTPIPNVIPTGGSIDASRSPGPTEFLAVPP
                     NPTQEPTPGANAAQHSGPSQAGPSPNAPSFSGAVAPRSDGSNLGASQATAGPNDPTHY
                     GPTQASPNPNAPTQEAPSEPSPNPNAPAALPPQEPTPGANAAQHYGPSENLSDASELP
                     PNRIVGQDPTNTTTHEVSGIVSAPVGNQYYTDRQGAQYGFVVAPAVDASPPPNAETFA
                     GDPSAAILANKQGAPVVR"
     gene            4630..4950
                     /locus_tag="VFQ35_04665"
     CDS             4630..4950
                     /locus_tag="VFQ35_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929953.1"
                     /translation="MTTRLEHDTRFAGVRTFTGQPETPSNVPAMNVSRHPVRELAAPS
                     PAAVVEVREAPRPVSAASTTPLEPRVAVPGTFVSTETADGRRVPIEQARELDEPIVIV
                     ETGD"
BASE COUNT          877 a         1727 c         1715 g          753 t
ORIGIN      
        1 gctgagtctc gatgggggtg ctgatgacga gcttgcggac ctgccggcgg gcgttcgcaa
       61 ctcgaaaatc agtttaggcg ccaagcgtcg cgccgcacgg atgcggatgg ggcggaagct
      121 gaggcgtatt gagcaaagcg agcgagttga agcgtcaggc tatccagctc tcgacgctgc
      181 cctgtccgag ctacggtcgt gcgcgtcacc actagacacg cgaggttccc ttccgtgtct
      241 ggtgggcgac gttcacaacg ggtttgcgct cgatcccgcg aacgcccgcg atcgcgccgg
      301 ccttcgcggc cggacccttt gcatgtgcgg acagccgttg tctgcccacg ctccgcattg
      361 gccgtttgac agcgccggct gcaacggctg gcagcagacg cccaccacgc ttcggggcga
      421 gcgcgatgtg aagcggcagc gaggcagact tccccgcggc gagggcggcc cggtgcccgc
      481 cggggcgccg gacgatgctc ccctcgggaa gatcgcggtt tgtgtaaccc ccctgagtag
      541 cgacgcgtgg gagcggcggt ttcggttgct ggacgcgctg cggccgatct ctccgcgccg
      601 taccgcgcgg tgtcgaaaac gccggtgctc gtcctctgtc caggtgcacc ggagtcaaga
      661 aggtgcggtc accctctcgg gggtcgtgac gtgcgcgtcg ccgtgggcat gcccggtttg
      721 tgcgtgcaac gtctacgcga gcagagcggc cgagatcacc aaggccatcg aacgctggaa
      781 cgagacgccc ggccacgctg tgtccctctt gacgctcaca gtacgtcacg cgttgggtga
      841 cgacttacgc gaggtgcggc gcgttctcac ggacgcgttt cgcgcgctat ggcgaggaca
      901 atccggcgga cgcctccgtc gagagctcgg ggtcacgcac ttcgtccgct cgctcgaggt
      961 cacgcacggc gcgaacggtt ggcatcctca tctgcacgtc ttgctcttcc agtcgcggaa
     1021 gcctgccgcg gacgccgtgg acaagctgaa aacacgctgg cgcaaaatct tcgagcatca
     1081 agcgagacgc ctccgcacgc gagcgctcgg actcacggga gagcttcgtg ctgaagccga
     1141 gagagccgcc gccacgctag aacacgcgac gccttcaata gagcacggcg cggatctccg
     1201 actgtcgcgc aacgctcgtt acatcgagaa gcttgggctc gagattgcaa gcatcgcgac
     1261 aaagagatcg caagcgagaa catcgcttac gccttgggag attgcctggc gggctgccga
     1321 cggtgatcgg cgcgcgcaga caatctggac cgcgtacacc gaagcgatgc acggcgcgcg
     1381 tcagctcacg tggtctcgtg gcgctagagc ggcgctcggg ttgtcgatgt cggacgaaga
     1441 tgaacggcac gctggagagc accacggcga gctcgtcgca tccgtaccag ccgcagtctg
     1501 ggatcgatgt tcgcgtgtag ctggttggac gatcgagctc ttgcgccggg cgcgtggaga
     1561 gcgcgctggc gaggagtgcg ctgtgttgat caaagcgtcg tgaggcggtc cgcggggtag
     1621 tgcgctccgg atgttccctt aggttccctt cgattccctt cggttccgct cgcgcggcgg
     1681 ccagctgcgc ccccactatc cgtccctttc cattcgttcg tcggcgtggc attctgtatg
     1741 tgtccgagac ggaaaaaagg gtgtgcgcag cggttctggc gcgtctcgca gtgaaccgag
     1801 aaagccgaag cacaccatgc gacgatctcg tgagtcccct ccccaaaagc atcacacgcc
     1861 gccgccgaag gagccgcgcg caccgaagac cgtggccgcg ctagtcgccc cggaaccgac
     1921 ggtcgaaaag aagcgcaagc cacgcgtcca caaggtgacc gcagacgaca tcctcaaggg
     1981 cgcgaaagcg cgcaccctcc tccgccgaac gtgcggcgag gcgacccgca aagccatctt
     2041 ctgagcaggg tcagaacatg gcgagcgaga ctacctcgct ctcgatcgct cgggccgcga
     2101 agtttctgaa cgtctcgcga tcgagggtca accagttcgt agcgtccggg cagctcacca
     2161 agagcgggcc gcccggacgc caaactatcc cgctcgtcga gctccgcgag cttctagacg
     2221 cgcgcaccgc ggaggctgca ggcgacgagt cggaggcgag tgcgacacgc ttcgagcgcg
     2281 agctacgggt ccagcgcgag aaggaacttg ccgactggga gcggcgtcag gctctcgacc
     2341 tcgaggaggc cgttttcgac gagcggaagc gagcaacgcg agagcgcgag cagctcgagc
     2401 gcgacgctct ccacgagatg aagaagcaca cgaaagtact cgagcgcgtc gcgaagtccc
     2461 tcgactctct ccagctcgtt ctcggcggag cactcgctgc cgctctcgtg ccgaacgaga
     2521 agaaggcgga ggcgtgggcg aatttgttcg gcgagtcgca gcctcgaaag caggcggaga
     2581 gccccgaacc ggtccgcgag cggctcggtg agctgagcgc cctcgtggcg agaggcagcg
     2641 gcgagccgct ccgagcggtg ccgaaggacg agcatccggg cccgccgctc ccgactgctg
     2701 aggaagtgaa ggacccggcc aattgggaaa aggtcttctc cacgtggcgg gagatcctcg
     2761 aggcacgctc tcggggaaag cagggagtca catcatgagg ttctccggcg aagaattcgg
     2821 actcgtagac acaacgttcg agcggaaggt ggccgaaatg gagccggcgc accctccgct
     2881 ttcggtcgtc gactcgcagc cgaagaaagg gccttggacg ggcaacaaca acctcggcta
     2941 tcggctcaag ttcgcgcccg acgcggacaa caggcagacg attctcaagc tccccgagtg
     3001 gggcttcccc gaggtgtgga ccgtttcgct cgcactctcc ggctttccgg gcggactctt
     3061 cgacggcttc ggcgtaaccg ggcacctcga gttcggcgta ggcggcgcga ccgaaaacat
     3121 cgccgtcgac tgggcgaacg gcacccaagt ttcgctcgcg atgaacgcgc tcaacgtgat
     3181 cgccgagttt tcgaacctcg acgttacgga ggagggtcaa gggctcgagc tcatcgttca
     3241 gcttgcgcgc ggcaaccgcc ctggcggctc gcgtcctcct acgctcacga tgcttcggga
     3301 cgagcccttc ggggccggcg ctgacacggg caacctggaa atcccggcgt tcgcctcgcg
     3361 catcatcgcc gtaccgagcg acccgaccgt cgcgggcgtc accaacttct actcgagcga
     3421 catcgtcatg gatctcgtga caggaaacgt tggttcgggt cacgtggttt ccgcggtctc
     3481 cggcgcgcag ctcgcgagcg tgcgcgggct cgacgtggtg ggggacgcgc gcttcgcgag
     3541 gctcatcaac cacgggacga acgcgacgcg cgtcaccgtc tacgcggagc tcgcggcctg
     3601 atggcgcgcg tcttcgcgaa gcgagttccg tacagctaca cgcgcgcggc gccgcacgtg
     3661 ccgtgcgccg gctcggctcc cgtcgtgctg ccgcgcgact cgggccccgt cgaggcggtc
     3721 gagcacaccg aagcgccctc gccggttccg cgacccgcgg aacggccgtg cgccggcacc
     3781 gtcgcaactg cactcgcgcc cgactcgccg ccgctcgaga gccggccgaa gaaaccgcga
     3841 tgggagcccg agcagcgtcc gcgccactcg cacgttccgt gcgcgggcac cgtcgcgacg
     3901 aaactgtgaa ggagctgacc gaatgtcctc agtcaacgtg agctacccgt gcgccggcac
     3961 cgtgccgacg ccgatcccga atgtgatccc gacgggcgga tcgatcgacg cgtcgcggtc
     4021 gcccggcccg accgagttcc tcgcggtgcc tccgaacccc actcaggagc cgacgcccgg
     4081 cgcgaacgct gcgcagcact cgggcccctc gcaggcgggc ccgagcccga acgcgccgag
     4141 cttctcggga gccgtggcgc cgcgctcgga cggctcgaac ctaggcgcgt cgcaggcgac
     4201 ggcgggcccg aacgacccga cgcactacgg ccccacgcag gcgagcccga acccgaacgc
     4261 acccacgcag gaggccccct ccgagccgtc gccgaacccg aacgcgccgg ccgcgctgcc
     4321 gccccaggag ccgactccgg gcgcgaacgc cgcgcagcac tacgggccgt ccgaaaacct
     4381 ctcggacgcc tcggagctgc cgccaaaccg catcgtcggg caggacccga cgaacacgac
     4441 cacgcacgaa gtgagcggca tcgtgagcgc gccggtcgga aaccagtact acacggaccg
     4501 gcaaggtgct cagtacgggt tcgttgtcgc gccggccgtc gacgcgtctc ccccgccgaa
     4561 cgcggaaacc ttcgcgggag acccgagcgc cgcaatcctc gccaacaagc agggagcccc
     4621 ggtggtgcga tgacgacgcg actcgagcac gacacgcgct ttgcgggggt gaggacgttc
     4681 accgggcagc cggagacgcc ctcgaacgtg cccgcgatga acgtcagccg acaccccgtg
     4741 cgcgagctgg cggctccctc gccggcagcc gtcgtcgagg tgagagaggc gccgcgaccc
     4801 gtgtccgccg cgagcacgac gcctctcgag ccgcgcgtgg cggtgcccgg aacgttcgtg
     4861 agcaccgaga cggcggacgg acgccgcgtg ccgatcgagc aggcgcgcga gctcgacgag
     4921 ccgatcgtca tcgtcgagac gggcgactga gctacactca ctttcgccat tcgtcggccc
     4981 ggtcgcgctc ttcacgcttg gcggcgcggc cggggagctg aacccctcgg gggtcgtttc
     5041 ctctctcgcg tttcgcggcg cgagtgcggc cc
//
LOCUS       DASTKS010000222         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1149827, whole genome shotgun sequence.
ACCESSION   DASTKS010000222 DASTKS010000000
VERSION     DASTKS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149827"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..425
                     /locus_tag="VFQ35_04670"
     CDS             180..425
                     /locus_tag="VFQ35_04670"
                     /inference="COORDINATES: protein motif:HMM:NF018545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HET9929954.1"
                     /translation="MDKETQTEVEAAAFRRLVQHFRERTDVQNIDVMNLAGFCRNCLA
                     KWYRAAAEERGVELSDSDAREAIYGMPYAEWKARYQK"
     gene            422..739
                     /locus_tag="VFQ35_04675"
     CDS             422..739
                     /locus_tag="VFQ35_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HET9929955.1"
                     /translation="MKSDGASGHRVERLEQLMLEELRALFEDELSDPRLAGVQPMAVV
                     LSPDYRHARVHCIAPNGGPGRHELEQAFLRASSVLRRRLADAIEFKRVPDLRFIVDAE
                     RTE"
     gene            910..1281
                     /locus_tag="VFQ35_04680"
     CDS             910..1281
                     /locus_tag="VFQ35_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9929956.1"
                     /translation="MRKLTTLVCRASWLSVCLTSIACGGAAVPQEKLTSAQAALKGAE
                     VGGAAEDPQAALYLKRSREELEKANALIADSMNEEAARVLDRSLADSELALALATEAH
                     QKKEAAQLKEQADELKSRIQK"
     gene            1278..2192
                     /locus_tag="VFQ35_04685"
     CDS             1278..2192
                     /locus_tag="VFQ35_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9929957.1"
                     /translation="MTKHAMTMRGALWMACAGAFSMACGAAVPPKELLDARAAYSRVE
                     KGPAAQLAPAELDTAKQSLARAEGRFADDGDSPETRDLAYIAERKALVAQAQAGIVQA
                     TREKEEIDKDFKKAQLATIDKTRAELEAERRAKEASNAQVQRTQDEVARTKADLAKEQ
                     KAREEAEKRAAAAMASLQEIAKVKEESRGVVITLSGAVLFATGKSELLPIARDKLTDV
                     AKAVKDQGFKEIVVEGHTDSVGSATKNEELSRQRAESVRSHLISQGIPADKIRAVGLG
                     SSRPVAENSTAEGRANNRRVEIIVTPIK"
BASE COUNT          449 a          712 c          757 g          359 t
ORIGIN      
        1 gtcactcgcc aatattcgtc taccgaaccg tttcgccccg cgtgaccgtt tgtaggtttg
       61 gcaggtttcg cgccggcttc gttcatgacc gctcggagca ataatgcggt ccccgtcggg
      121 ctatttcgat gtaggcagcc cgagccaagg gcgttcctcc gatgtagtct gcaatgacca
      181 tggacaaaga aacgcaaaca gaggtggaag ctgcggcctt tcgtcgactg gttcagcact
      241 ttcgagaacg gaccgacgtg caaaacatcg acgtgatgaa tctcgccggt ttctgccgaa
      301 actgcctcgc caagtggtac cgcgccgcgg ccgaggagcg gggcgtcgag ctcagcgact
      361 cggacgcacg agaagccatc tacggtatgc cctacgcgga gtggaaggca cgctatcaaa
      421 aatgaagtcg gacggggctt ccggtcatcg cgtcgagcgc ctcgagcaat tgatgctcga
      481 agagctccgc gcgctcttcg aggacgagct ctcggaccct cgtttggccg gcgtccagcc
      541 gatggcggtc gtactctctc ccgactaccg ccacgcgcgc gtccactgta tcgcgccgaa
      601 cggaggcccc ggccggcatg agctcgagca ggcattttta cgcgcttcga gcgtcctgcg
      661 aaggcgcctc gcggacgcca tcgagttcaa gcgcgtcccg gacctgcgct tcatcgtcga
      721 tgccgagcgg accgagtgac caagtccggg aatccggatc cgcacgaggt gcgagggtgt
      781 gagagaaagt gcaggcgcta tttttcgctg agctcgccgc gcgcctcggc gagcccgctg
      841 gcccgttgga cgaggcgcga ggcaccacct ttacctcgtt gccgggttga gctttgatgg
      901 cgcctagtca tgcggaaatt aaccactctg gtttgccggg cctcatggct ctcggtgtgc
      961 cttacttcaa tcgcttgcgg cggagccgct gtcccgcaag agaaactgac ctcggcccaa
     1021 gccgcgctca agggcgcgga agtgggcggt gcggcggaag atccccaagc agcgctctac
     1081 ctgaagcgct ctcgcgagga gctcgaaaag gcgaacgcgc tcatcgccga ttcgatgaac
     1141 gaagaagccg cgcgggtgct cgatcgctcc ctcgctgact cggagctcgc gctcgcgctc
     1201 gcgaccgaag cgcatcaaaa gaaggaagcg gcccaattga aggaacaggc ggacgagctc
     1261 aagtcgagga tccaaaaatg acgaagcatg cgatgacgat gcgtggcgcg ctgtggatgg
     1321 cgtgcgccgg tgctttctcg atggcgtgtg gcgccgcggt tccgccgaag gagctgctcg
     1381 acgcgcgtgc cgcttactcg cgcgtcgaaa agggtccggc ggcgcagctc gctcccgcgg
     1441 agctcgacac ggcgaagcag tcgttggctc gcgccgaagg gcgcttcgcc gacgacggcg
     1501 attcccccga gacccgcgat ctagcctaca tcgccgagcg caaggcgctc gtcgcgcagg
     1561 cgcaggccgg tatcgtgcag gcgactcgcg aaaaagaaga gatcgacaag gacttcaaga
     1621 aggcgcagct cgcaaccatc gacaagacgc gcgccgagct cgaggcagag cgccgcgcga
     1681 aggaagccag caacgcgcag gtgcagcgca cgcaggacga agtcgctcgc accaaggcgg
     1741 atctcgccaa ggagcagaag gcgcgcgagg aagccgagaa gcgcgcggcc gcggcgatgg
     1801 cgagccttca ggaaatcgcg aaggtcaaag aggagtcgcg cggtgtggtc atcacgctct
     1861 ccggcgccgt gctgttcgcg accggcaaga gcgagctact cccgatcgct cgcgacaagc
     1921 tcaccgacgt cgccaaggcg gtcaaggatc agggcttcaa ggagattgtc gtcgaagggc
     1981 acaccgactc cgtcggcagc gccaccaaga acgaggagct gagccgccag cgtgccgaat
     2041 cggtgcgcag ccatctgatt tcgcaaggca tccccgccga caagatccgc gcggtgggcc
     2101 tcggttcgtc ccgccccgtc gccgaaaact cgacggcgga agggcgcgcg aacaaccggc
     2161 gcgtcgagat catcgtcacg ccgatcaagt agcgcttact tgcggtcgcg atagaggtcc
     2221 gcgacgtcgt gttgaaccgg aggtggagga ggcggcggtg ctgaagaagg cgctgcc
//
LOCUS       DASTKS010000223         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_35434,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000223 DASTKS010000000
VERSION     DASTKS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35434"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFQ35_04690"
     CDS             <1..957
                     /locus_tag="VFQ35_04690"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET9929958.1"
                     /translation="REQVQSMGADFLEVALKEEGSGVGGYAKEMSPEFIAAEMALFLA
                     QAKEVDIIITTALIPGKPAPLLINEETVAAMKPGSVIVDLAAESGGNCAFTRPGEVVR
                     QNGVTIIGYVDMPSRLAPLASQLYGSNLVHLLSDMGGAEKWRIDLDDQVVRGAIVLKE
                     GELMWPPPRIEPSPAAAPKKAADKPVPTPSAGKAGGHGHGASTSDEPKSPIGLLVVAA
                     LVTALGAVAPREFLSHFTVFVLACFVGWQVVWNVTPALHTPLMSVTNAISGIIVVGGM
                     LQLTSQPRPEVTWIGAAAVLLACINIAGGFLVTQRMLKMFRR"
     gene            966..2390
                     /gene="pntB"
                     /locus_tag="VFQ35_04695"
     CDS             966..2390
                     /gene="pntB"
                     /locus_tag="VFQ35_04695"
                     /inference="COORDINATES: protein motif:HMM:NF006974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HET9929959.1"
                     /translation="MTESLSTVAYLIAGVLFILSLSGLSTQLTARRGNLYGMIGMLAA
                     LLVTAASNAERMNFGVLAVAIGVGGAIGAMLALRVEMTAMPELVAILHSFVGLAAVLV
                     GFGTYLMPGPEGGAERLIHELEVFIGVFVGALTFTGSVVAFGKLRGTFRSKPLLLPGR
                     HLLNLVLVLGCVALGVHFVGNDVDSGIVALGAQAVIAGVIGVHLVMAIGGADMPVVVS
                     MLNSYSGWAAAAAGFMLSNDLLIITGALVGSSGAILSYIMCRAMNRSFLSVIFGGFGA
                     DEGTAPARDASAPQGEVTPIQAVETADLLREAKSVVVVPGYGMAVAQAQYPLYEIAEW
                     LRQRGKVVRFAIHPVAGRLPGHMNVLLAEAKVPYDIVLEMEEINDDFPETDVVLVIGA
                     NDIVNPSALDDTSSPIYGMPVLEVWKSKVTVVMKRSMASGYAGVENPLFFLENTRMLF
                     GDAKKNVDAILTSLRSEAGAAAAE"
BASE COUNT          496 a         1037 c          949 g          558 t
ORIGIN      
        1 cgcgagcagg tgcagagcat gggcgccgat ttcctcgagg tcgcgctcaa ggaagaaggc
       61 tccggcgtcg gcggctacgc gaaggagatg agccccgagt tcatcgccgc cgagatggcg
      121 ctctttctcg cgcaggcgaa agaggtcgac atcatcatca ccaccgcgct catccccggt
      181 aagcccgcgc cgctgctcat caacgaagaa accgtcgccg cgatgaagcc cggctccgtg
      241 atcgtcgatc tcgccgcgga gagcggtggg aactgcgcgt tcacccgccc cggtgaagtc
      301 gtgcggcaga acggcgtcac catcatcggt tacgtcgaca tgccgagccg cctcgctccg
      361 ctcgcgagcc aactctacgg ctcgaacctg gtgcacctgc tgagcgatat gggcggggcc
      421 gaaaagtggc gcatcgatct cgacgaccag gtcgtgcgcg gcgccatcgt gctgaaagaa
      481 ggcgagctca tgtggccgcc gcctcggata gaaccgagcc ccgcggccgc tccgaaaaag
      541 gccgccgaca agccggttcc gacgccgtca gcagggaaag cgggcggcca cggccacggc
      601 gcttcgacct ccgacgagcc gaagagcccg attgggttgc tcgtcgtcgc ggcgctggtc
      661 acggctctcg gcgcggtcgc tcctcgtgag ttcctttcgc atttcacggt cttcgtgctc
      721 gcttgtttcg tcggctggca agtggtctgg aacgtcaccc cggcgctcca tacgccgctc
      781 atgagcgtta ccaacgccat cagcggcatc atcgtcgtgg gcggcatgct gcagctcacg
      841 tcgcagcccc ggcccgaggt cacctggatc ggcgctgcgg ccgtgttgct cgcgtgcatc
      901 aacatcgcgg gcgggttcct agtcactcag cgcatgctga agatgttccg tcgttaggag
      961 ccgcgatgac cgaaagcctt tcaaccgtcg cttacctcat cgctggcgtg ctcttcatcc
     1021 tgagcttgtc ggggctcagc actcagctca ccgcgcgccg cggcaacctc tacggcatga
     1081 tcggcatgct cgccgcgctg ctcgtcaccg ccgcgagcaa cgccgagcgc atgaacttcg
     1141 gagtgctcgc ggtcgccatc ggcgttggcg gcgccatcgg cgcgatgctc gcgctgcgcg
     1201 tcgaaatgac ggccatgccc gagctcgtcg cgatcctgca tagcttcgtg gggctcgcgg
     1261 ccgtgctcgt cggtttcggc acttacctga tgcccggacc cgagggcggc gccgagcgct
     1321 tgatccacga gctcgaggtg ttcatcggcg tattcgtcgg cgcgctcacc ttcaccggct
     1381 cggtcgtcgc gttcggcaag ctgcgcggca ctttccgcag caagcccttg ctcctgccgg
     1441 gtcgccacct gctgaacctc gtcctcgtcc tcgggtgcgt cgctctgggc gtgcacttcg
     1501 tcggcaacga cgtcgacagc ggcatcgtgg cgctcggcgc gcaggcggtg atagccggtg
     1561 tcatcggcgt tcacctcgtg atggcgatcg gcggcgcgga catgcccgtc gtcgtctcca
     1621 tgctgaacag ttactcgggt tgggccgcgg ctgccgccgg cttcatgctc tcgaatgacc
     1681 tcttgatcat cacgggcgcg ctcgtcggct cgagcggcgc catcctcagc tacatcatgt
     1741 gccgcgcgat gaatcgctcg ttcctgagcg tcatcttcgg cggctttggc gcggacgaag
     1801 gcacggcccc ggcgcgcgac gcgagcgctc cgcaaggcga ggtcaccccg attcaagcgg
     1861 tcgaaaccgc cgatttgctg cgtgaggcga agagcgtcgt cgtcgttccg ggctacggca
     1921 tggccgtcgc tcaagcgcag tacccactct acgaaatcgc ggagtggttg cggcagcgcg
     1981 gcaaggtcgt gcgcttcgcg attcaccccg tcgcggggcg cctacccggg cacatgaacg
     2041 tgctgctcgc cgaagccaag gtcccgtacg acatcgttct cgagatggaa gagatcaacg
     2101 acgacttccc cgagaccgac gtcgtactcg tgatcggcgc gaacgacatc gtgaacccga
     2161 gcgctctcga cgacacgtcg agtcccatct acggcatgcc cgtgctcgag gtgtggaagt
     2221 cgaaggtcac cgtcgtcatg aagcgcagta tggcgagcgg ctacgcgggc gtggagaacc
     2281 cgctgttctt cctcgagaac acccgcatgc tcttcggcga cgcaaagaag aacgtcgatg
     2341 ccatcctcac gagcctccgc agcgaggccg gcgccgcagc cgccgagtaa ctcggtgtgc
     2401 ccatgctccg ccgtccgagt cacgaatcgt ggcgaccggg cgcgaagcta tgtcgaataa
     2461 cccgagtcgt cccacgcctc ggcccccggt ggccgtcagg ccgagaccgc atcgatcgaa
     2521 gaatgctttc acccgcgacc ggttaccgct tgatcggtgc ttcgacgtgt caccgcactc
     2581 atgcatctcg tcagatccgt tgagttcgaa accgcgctgc attcgcgcgt gtaggactcg
     2641 gcacgtaagg gttctttccc ctcgtgcggc ggatggctta gtcccacccg taagcggcct
     2701 cttttggggc ccgagagccc cagagcaata tttcgtccct gaggaccaaa tcatgaggcc
     2761 tcccggccgg ccaaatgttt gtttgcgtgc ccccctcgct gagatatctt gagccaagcc
     2821 cttagcggtc gttgaatcga cccaacgggt aaagcttcga gcgtcagctt cgaagcgcgt
     2881 ctcgcggact ctatggattc gagcaagccc gacaatcacc tcctcgacga gctgctcgga
     2941 atcgatccac gcgttttcga cgaggaggag cgcaccatcg tgctccctcg ctccgagccg
     3001 ccgccgccgg atccgcacgc ctcgtcgcct tcgcgcatgc
//
LOCUS       DASTKS010000224         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1373372, whole genome shotgun sequence.
ACCESSION   DASTKS010000224 DASTKS010000000
VERSION     DASTKS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373372"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFQ35_04700"
     CDS             complement(<1..709)
                     /locus_tag="VFQ35_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET9929960.1"
                     /translation="MAAAITSQLLPTRPEPGRSPDAASVTAEEKVAFARALGRVESHP
                     GGTLVSPVRTRLDAGQASEALRTAWRAVTGEEPSDKTVRVLTAQWAHETAHGASMFNY
                     NFGGIKGVGPSGLSVSQRTREGFGETERTITDRFRAYQTAEEGATDYVRLLSKRFPEA
                     VQAARDGDPSAFVHALKNRGYFTGDLGAYTRSVVALSGIELPSSASPAPPRLNAAPLV
                     LAAANKVEPSELTPLSTT"
     gene            complement(753..1070)
                     /locus_tag="VFQ35_04705"
     CDS             complement(753..1070)
                     /locus_tag="VFQ35_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929961.1"
                     /translation="MRSLNPGGPLRVSRNECRVRPEHLKDLGKIQDLLTALNDPYANI
                     GKLVAAIPVLSARCVILAGDRSKLEINSVDHALTLIGNKGLESELLTLLEDLTILKAE
                     LET"
     gene            1415..2155
                     /gene="flgF"
                     /locus_tag="VFQ35_04710"
     CDS             1415..2155
                     /gene="flgF"
                     /locus_tag="VFQ35_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HET9929962.1"
                     /translation="MSTGIWSAASGAVAQSASLDTAANNVANASTPGFRAETAIFRQT
                     LVNAIGQNSGTQSQRFAVTRTTSPDMRQGQIVHTGRNLDVAISDDRGFFAVSTPNGER
                     FTRAGSFRVSVDGTLLTADGHTVLGPSRRPVQVSPTATSLEIGPDGAISASGEPTGAT
                     IGVFTFGNLAGLEKEGDILFRARPDAGAPRIHDGKLESHAIEQSNANAVSSMTTLVNA
                     SRQFEMVAKVIEAFSQIERRAATDIMKR"
BASE COUNT          507 a          867 c          902 g          492 t
ORIGIN      
        1 gagtagtcga cagcggggtg agctcgctcg gctcgacctt gttcgcggcc gcgagcacga
       61 gcggagctgc gttcagtcgt ggtggcgcgg gcgacgcgct gctcggcagc tcgataccgg
      121 agagcgcgac gacagaccgc gtgtaagcac caaggtcgcc agtgaagtag ccgcggttct
      181 tcagcgcgtg cacgaaggcg gaaggatcgc cgtcgcgcgc cgcctgcacc gcctcgggaa
      241 aacgtttcga gagcagccgc acgtaatcgg tcgccccttc ttccgcggtc tggtacgcgc
      301 gaaagcgatc cgtgatggtg cgctcggtct cgccgaagcc ctcgcgcgtt cgctgcgaaa
      361 cgctgagccc gctcggcccg actcccttga tgccgccgaa gttgtagttg aacatcgacg
      421 cgccgtgcgc ggtctcgtgc gcccactggg cagtgagaac gcgcaccgtc ttgtcgctcg
      481 gctcttctcc ggtcacggcc cgccacgccg tgcgcagcgc ttcactcgcc tgccccgcgt
      541 cgaggcgtgt acggactgga cttaccagcg tgccgcccgg gtgcgactcg acgcgtccga
      601 gcgcgcgcgc gaacgcgacc ttctcctccg ccgtcacact ggccgcgtcc ggggagcgtc
      661 ccggttcggg ccttgtcggc aagagctggc tggtgatggc ggctgccacg ctccttgaaa
      721 cggcaagaag cgggccagcg gtgtttacgc cttcacgtct cgagctctgc cttcaaaatc
      781 gtgagatctt cgagaagcgt caacagctcg gattccaatc ccttgttccc aatgagcgtg
      841 agagcgtgat cgaccgaatt gatttcgagc ttcgagcgat cgcccgcgag gatgacgcag
      901 cgagcggaga gaactgggat cgcggccacg agcttgccga tgttcgcgta cggatcgttc
      961 aaggcggtga gcagatcctg aatcttgccg agatctttca aatgctctgg gcgtacgcga
     1021 cactcgttgc gactcacgcg caaggggccg ccagggttca gggagcgcat cgctttcttg
     1081 agtgtgcgag atcgaggcca gcgagcaagt gaagtcgcgg aagcagacat cggcgagaca
     1141 accgcggagc ttggctagta gcgtcaacta gctgacgagc tgccgtcaag cgattcgcag
     1201 agagcgcctg cgcgtcgcct ccgcatcgtg tgtagttccg cgtgcgaggc gcgcgctcat
     1261 ttctccatcc cgatcggcgg cgagcggcgc tcgacggccg tagagcgcga cgcgcggtcg
     1321 agttcgagcg cgacgagacc ggcacgcgag gcggcgcggc gctcctgagc gtgcgagccc
     1381 gagttggcgc ggggcttgct gtccctctag cgcaatgagc accggaatct ggtccgccgc
     1441 gtcgggcgcc gtcgcgcagt ccgcgtcctt ggacactgcc gcgaacaacg tcgcgaacgc
     1501 ttcgacgcca gggttccgag cagagacggc catcttccga cagactttgg tcaacgcgat
     1561 cggccagaac agcggaacac aatcccagcg cttcgccgtt acgcgtacga cgagcccgga
     1621 catgcgccag gggcagatcg tgcatacggg ccgtaacctc gacgttgcga tatcggacga
     1681 tcgggggttc ttcgccgtga gtacgccgaa cggggagcgc ttcactcgcg ccggaagctt
     1741 ccgggtgagc gtggacggca cattgttgac cgctgacgga cataccgtgc tcggacccag
     1801 ccggcggccg gtccaggtct cgccaaccgc gacatcgctc gaaatcggcc cggacggcgc
     1861 aatctctgcc tcgggagagc cgactggggc aacgatcgga gtctttacgt tcggtaacct
     1921 ggcaggcctc gagaaagagg gcgacatcct atttcgcgct cgcccggacg cgggcgcccc
     1981 ccgcattcac gacggcaagc tcgagtccca cgcgatcgag caatcaaatg ccaacgccgt
     2041 gtcttccatg acgacgctgg tgaacgcctc gcggcaattc gaaatggtcg ccaaagtgat
     2101 tgaagctttt agtcaaatcg agcggcgcgc agctacggat atcatgaagc ggtagttggg
     2161 ccgccgattt cgcggaattc tccgggtttt ccgcgcgacc ggcgaaaatc cctgcttcgt
     2221 gctcgtgatt gtggcggatt gagtgtttga aacgcctact tcgcggtgag ttcgtcacgc
     2281 ccgccgccgc attcgcgccg tcttccctga ttttgcggca atttctgcgc aaatgggctc
     2341 gcgggagcgc tgctatcggc aagcagaaaa tgtgaagatg ctgaattggt gatggccgca
     2401 gcggcacaca ccttcgccac agcggacacc ccgacgacgg gtgccgctta tgtctcgcga
     2461 cggaaaagcc gcttggtttg acggatagcg cacgcccaga cggcgtcaag cgattgggca
     2521 tgcgcgaggc acgcgaacga gctaaccttg gcttgggcct gagagaaata gggttccaag
     2581 tcgaaacacg agagttcgtc ttgacgaccc acatcaaggt atgagacagg ggcccgagca
     2641 gactaatggc gcacggtcgc gatttacgac gtgccatcga tcgcctcgtc gccgacttga
     2701 cccgcatcgc tgaacgagcg attgcgatcg agatcgagcg agcgatcgcg ctggcaattg
     2761 acccaatc
//
LOCUS       DASTKS010000225         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_927398, whole genome shotgun sequence.
ACCESSION   DASTKS010000225 DASTKS010000000
VERSION     DASTKS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927398"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFQ35_04715"
     CDS             <1..903
                     /locus_tag="VFQ35_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9929963.1"
                     /translation="GPASHSVAELSRLMIGDEPPQIARRPPEYGSTALRVEGLELPKQ
                     HEFGVALRAVSLQVRAGEILGVAGVTGNGQSELLAALAGETLLASASHLELFGVDLRK
                     LGPAQRRALGLRYVPEERLGVATVPSLSLSENVLLTRRPREKSSGFVPWGALRALTSE
                     LLRRFGVKASGPEANAASLSGGNLQKYVVGREVGARPTLLMLAQPTWGVDVGAAASIK
                     GELFALRDAGAALLIVSEDLDELFELSDTLCVMANGSLSPLVDASIATRSQIGEWMSG
                     LWQGALAAASTDERSELARAHAKN"
     gene            878..1960
                     /locus_tag="VFQ35_04720"
     CDS             878..1960
                     /locus_tag="VFQ35_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929964.1"
                     /translation="MLELTPRTEAPAWLRLASPIAALVVTLSFGILLFHFVGKEPFAA
                     LELFFWEPLKNARALGELALKASPVLLIALGLSLCFRTNVWNIGAEGQFILGAIGASW
                     VSLHARGESGPWLVAAALLAGALGGIAWAALCAFFREHFHASEILTSLMLVYVAEQLL
                     SYLVHGPWKDPNGYNFPQTITFLDAARLPRLGSGSRAHAGLLLVPLAIAGFWLFQRHS
                     HAGYRLRVVGLSPRAALYAGFAKSRAIYTALLASGATAGLAGAMEVCGPVGQLTPHVP
                     LGYGFAGIIAAFVGRLHPIGVTLSSLLMSLFYIGGELSQSRLGLPKSVTGVFEGVLLF
                     ALLACDTLVQNRVRLTWPFGSRSITP"
     gene            1957..2877
                     /locus_tag="VFQ35_04725"
     CDS             1957..2877
                     /locus_tag="VFQ35_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9929965.1"
                     /translation="MNELALFLSASLASGTPLALAALGLLLNERAGVINLGTEGLMLV
                     AAVTGFATTVHSHHPALGLLAGAAASALLSALLGALVIFLDTNQYATGLALSLFGAGF
                     SAFVGVPYTQESIPAHASTGIPVLAHVPFIGPALFSHHPLVYGAMILAAAETWFFFRS
                     KPGLVVRAIGEAPEAAQALGYRVRSIRFKTVVLGGALVGLAGAYVSVVATPLWVEGMI
                     AGKGWIALALTTFATWRPMRVLLGAYLFGAVTMLQLHLQGRGISLPVEFLTALPYLST
                     ILVLVVISRNPRFIAANIPASLGKPFHSGA"
     gene            2882..>3161
                     /locus_tag="VFQ35_04730"
     CDS             2882..>3161
                     /locus_tag="VFQ35_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929966.1"
                     /translation="MLNPLATTRALAFLFVPSLVAVSCSKKPTNEAAPSASIAAAVPA
                     KETGPMKVAFAYVGPVGDAGWTFAHDKARKQAEAALAGKVKTSFVENVP"
BASE COUNT          466 a         1050 c         1006 g          639 t
ORIGIN      
        1 ggtccggcga gtcacagcgt cgcggaactt tcgcggctga tgatcgggga tgagccacca
       61 caaatagcgc gtcggccacc cgaatatggg tcgactgccc tacgcgtcga aggcttggag
      121 ctgccgaagc agcacgaatt cggagtggcc ctgcgcgcgg tgtccttgca ggtgcgcgcg
      181 ggcgagatcc tcggcgtggc cggggtcact gggaacggac aaagcgagct tctcgcggcg
      241 ctcgccggcg aaactctgct cgcaagcgcg agccacctcg agctttttgg cgtggatctt
      301 cgaaagcttg gccccgcgca acgccgcgct ctcggtcttc gttacgtccc cgaagagcga
      361 ctcggggtcg cgacggtgcc ttccttgtcg ctcagcgaga acgtgctcct cacgcggcgc
      421 ccgcgcgaga agtcttcggg gttcgtccct tggggcgccc tgcgagccct gacttccgag
      481 ttgctccggc gcttcggcgt caaagcgagc ggcccggagg cgaacgcggc gagcctctcc
      541 ggtggtaatc tgcaaaagta cgtggtcggg cgagaagtgg gcgctcgacc cacgctactg
      601 atgctcgctc aacccacctg gggcgtggac gtcggcgcgg ccgcgagtat caagggcgag
      661 ctcttcgcgt tgcgcgacgc cggggcggcc ctgctcatcg tgagcgaaga tctcgacgag
      721 ctgttcgagc tcagcgacac gctctgcgtc atggcgaacg gctcattgtc gccgctggtc
      781 gacgcctcga tcgccacgcg ctcccaaatc ggtgaatgga tgagcgggct ctggcaaggc
      841 gcgttggcag cggcctctac cgacgaaagg agcgagcttg ctcgagctca cgccaagaac
      901 tgaggcgccg gcgtggcttc gtctcgcgtc gccgatcgca gcgctcgtcg tcaccttgtc
      961 gttcggcatt ctgctgtttc acttcgtcgg caaagagccc ttcgccgcgt tggagctgtt
     1021 cttttgggag ccattgaaga acgcgcgcgc tctcggcgag ctggcgctca aagcgagccc
     1081 agtcctcctc atcgcgctcg gcctttcgct gtgctttcgc accaacgtct ggaacatcgg
     1141 cgcagaaggt cagttcatcc taggcgccat tggtgcaagc tgggtctcgc ttcacgctcg
     1201 tggcgagagc gggccatggc tagtcgcggc tgccctgctc gccggagcgc tcggaggcat
     1261 cgcctgggcg gccctgtgcg cgttctttcg cgagcacttt cacgcgagtg agatcctcac
     1321 gagcttgatg ctggtgtacg tcgccgagca gcttctgagc tacctcgtgc acggcccgtg
     1381 gaaggaccca aacggctaca acttcccgca aaccatcacc ttcctcgacg cggcgcggtt
     1441 gccgcgattg ggcagcggct cacgcgctca cgcgggtctc ttgctcgtcc cattggcgat
     1501 cgcagggttt tggctgtttc agcgccatag tcacgcgggc tatcggctgc gcgtcgtcgg
     1561 gctctcgccc cgtgcggcgc tctacgccgg gttcgcgaaa tcccgcgcca tctacacggc
     1621 gctcctcgcc agtggcgcca ccgcagggct cgctggcgcg atggaagttt gcggccccgt
     1681 cggccaactc acgccgcacg tgccgctcgg atacggcttc gcagggatca tcgccgcctt
     1741 cgtgggccga ttgcacccga tcggagtcac gctgtcgtct ctgctgatga gcctttttta
     1801 catcggtggg gagctctcgc aatctcgtct cggtctaccg aaatcggtga ccggcgtctt
     1861 cgagggagtc ttgttgtttg cgctgctcgc gtgcgacacg ctggtacaga atcgagttcg
     1921 cctcacctgg ccgttcggct cgaggagtat caccccgtga acgagctcgc gctcttcctc
     1981 tctgcctcgc tcgcgtcggg aactccattg gcgctcgccg ccctcggact gttgctgaac
     2041 gagcgcgcgg gggtcatcaa cctcggtact gaaggcttga tgctcgtcgc ggccgtcacc
     2101 gggttcgcaa ccacggtgca ctcgcatcat cctgctctcg ggttgctcgc gggcgcggct
     2161 gcttcggccc tgctctcggc gctgctcggc gcgctcgtca tttttctgga cacgaaccag
     2221 tacgccacgg ggctcgcgct gtcgctgttc ggcgccggtt tctcggcgtt cgtcggtgtt
     2281 ccgtacactc aggagagcat cccagcccac gcttccacgg gcattccggt gctcgcgcac
     2341 gtgccgttca tcggacccgc gctgttttca caccacccgc tcgtgtacgg cgcaatgatc
     2401 ctcgctgccg ccgagacctg gttcttcttt cgaagcaagc cagggctcgt cgtgcgcgcg
     2461 atcggggaag ccccggaggc ggcgcaggcg ctgggttacc gcgtgcgctc gattcggttc
     2521 aaaaccgtag tcctcggcgg agctttggtc gggctcgccg gtgcctatgt gtcggtggta
     2581 gcgacccctc tctgggtgga aggcatgata gccggcaagg gttggatcgc gctcgcgctg
     2641 acgacgttcg cgacatggcg tcccatgcgt gtgctgctcg gcgcttacct gttcggagcc
     2701 gtcacgatgc tgcagctgca cctccaaggt cgcggcatca gcttgcccgt cgagtttctt
     2761 accgcgctgc cctatctctc gaccattctc gttctcgtcg tcatctcgcg aaacccccgc
     2821 ttcattgcgg cgaacatccc ggcctcactc ggcaagcctt ttcactcagg agcttgaaac
     2881 catgctgaac ccgctcgcaa ccactcgcgc gctcgctttc ctcttcgtac catcgttggt
     2941 cgcagtgtct tgcagcaaga agccgacgaa cgaagctgcc ccgagcgcca gcatcgcggc
     3001 cgcggttccg gcaaaggaaa ctggacccat gaaggttgcg ttcgcttacg tcggtccggt
     3061 cggcgatgcg ggctggacgt tcgcgcacga caaggcgaga aagcaggcag aagccgcgct
     3121 cgccggaaag gtgaagacga gcttcgtcga aaacgtgccc g
//
LOCUS       DASTKS010000226         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_257542, whole genome shotgun sequence.
ACCESSION   DASTKS010000226 DASTKS010000000
VERSION     DASTKS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257542"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1759
                     /locus_tag="VFQ35_04735"
     CDS             323..1759
                     /locus_tag="VFQ35_04735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9929967.1"
                     /translation="MDAGRVLAGRYRLVSKLGSGGMGTVWSAHDLHLNADVAVKLIDP
                     KLMSSPEALVRFRREAQAAATIRSTHVVQIFDHGVDGQEPFIAMELLHGESLAERLTR
                     VGRLPPEVTARLLGQVARALALAHQRNIVHRDMKPDNIYLVHEGDEDIAKVLDFGIAR
                     AQGLAEGGPAEVATRTGAVLGTPFYMSPEQANAHALDARTDIWAFGVIACECLTGRRP
                     FSADTLAALFHAICMAPPPVPSSLGAVPEGFDAWFARATARNLGERYASIREAADDLK
                     HVCLSVAVHAERASNSASPNVSTSSAGNVSTAGEVGTVTAVQLGTPDARPSHRLRNGI
                     AAALFIVVVLGTGAAFRRYVASGVASGAEPRVTVASARDTERVENGVSSSAPSSALPI
                     NGDATASGAGEVPKPTTSTTEEPAPPTRPAPTRAATARAGANAVASSTAVPTPVGAPK
                     PDRTGSKVAPAKRAPNPSPKRDDNASGI"
     gene            1761..2828
                     /locus_tag="VFQ35_04740"
     CDS             1761..2828
                     /locus_tag="VFQ35_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929968.1"
                     /translation="MRIRTRLFAVAFASSLLGTAPALALSDSDKEAIRVLSNQAAADF
                     DQGRYEAARDKFERAYALASVPKLAVWLARSQEKLGRWVEAYELYRQAVSLQPNELWK
                     GTVQQNAQSDARAAIDALYARIPRLTLSVEGAPLAEVKVTIDGAEVPSGLLSVERLLN
                     PGEHDVVARFGEQSLNEHVSLSERDVKALALRFTSTQPTPAAAAPIAATAVSSSPLPA
                     PPAIVPPQTQTQTQTPAPGALAGSAARRPGAGQRTVGWVSVGLGAAGIGLGAVTGVLL
                     AGKYSDLTSKCELRVCPAERKSDVESYDRLRTLSSVGFIAGGALTALGVTLLLTAPKS
                     GGTTGIELVVSARDATLLGVF"
     gene            2828..>3643
                     /locus_tag="VFQ35_04745"
     CDS             2828..>3643
                     /locus_tag="VFQ35_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929969.1"
                     /translation="MSTRRLSFAAALLGVGCAFPSFEVREQSGGAGGASRGGSTAGGA
                     GGKLGAGGAPSGGATGAPGGKSGGASSSQGGASSSQGGASTSGGGRGGSSSGGKSNGG
                     ATSGGAPGGGAPGGGTTSNGGSHGGAPTAGAPGNCGGASSTARGPFTCGSTPATCNQI
                     SSFPLDGAWGSGDFTGGLVVYGKGLSRDMTQTEVLHITGTVDDYVGFAFWFSTCSSLD
                     TFKGVQFKLSNMLGAGKTVRFQLQTNADFPWQSDPNAGKGACTATTCDGVWGEC"
BASE COUNT          651 a         1154 c         1199 g          639 t
ORIGIN      
        1 cggtctcctc cactcgctga cagcctagcg tagaagaccc gagcgcgcag aggacgcgcg
       61 tcacttccgg cgcacctcgc gagcgcttgc tgctacaggt gcacacgctc gccttcgtga
      121 ccgcactccg tcggcaacgc cgatgttttg gggagtcaat ctcaatcttt ccgccactga
      181 gaacggtcac catggctgtc cacgcaattc gatgattgcc atcgctcgaa aacggactgc
      241 gcggattcca atagtcattt tcttggacac agagtccatt cgcggttcta ggccttcgcc
      301 aaacggtgct aaaagagcac gaatggacgc gggacgcgtt ttggcagggc gttatcggct
      361 cgtctccaag ctgggaagtg gagggatggg gaccgtctgg agcgcgcacg atttgcacct
      421 gaacgccgac gtagccgtca agctgatcga tccgaagctg atgtcatcgc cggaagcgct
      481 cgtgagattc cggcgcgaag cgcaagccgc ggcgacgatt cgcagcaccc acgtggtgca
      541 aatattcgat cacggagtcg acgggcaaga gccgttcatt gccatggagc tcttgcatgg
      601 cgagagtctt gccgagcgac tgacgcgtgt gggtcgacta ccgccggaag taaccgctcg
      661 cttgcttggg caggtagcgc gcgccctcgc cctcgctcat cagcgcaaca tcgttcaccg
      721 cgacatgaag ccggacaaca tctacctggt tcacgagggt gacgaggaca tcgccaaggt
      781 gctcgacttc ggaatcgcgc gcgcacaggg cctcgctgaa ggtggtcccg ctgaggtcgc
      841 cactcgcacg ggcgcggtct tgggaacgcc cttttacatg agccccgagc aggcgaatgc
      901 gcatgcgctc gatgcgcgca cggacatttg ggccttcggc gtcatcgctt gcgaatgcct
      961 cactgggcgg cgccccttca gcgccgatac gctggcggcg ctgttccacg caatctgcat
     1021 ggcacctccg cccgttccgt cttctctcgg cgcggttccg gaggggttcg atgcgtggtt
     1081 cgcgcgagcc acggcgcgca acttgggcga gcgctacgct tcgattcgcg aagctgcgga
     1141 cgatttgaag cacgtgtgtc tcagtgtagc ggttcacgcc gaacgtgcgt cgaactccgc
     1201 gtcgccgaat gtctctacgt cctcggcagg caacgtttcg accgcgggtg aggtgggcac
     1261 ggtgacggcg gttcaactcg gcacgcccga cgcgaggccg tcgcatcgct tgcgcaatgg
     1321 gattgcggcc gcgctcttca tcgttgtggt cctcggtacc ggagcagcgt ttcgacgcta
     1381 cgttgcgtct ggagttgcgt ccggggccga accgagagtc acggtcgcta gtgcgaggga
     1441 caccgaacga gtcgagaacg gtgtctctag ctcggctcca tcgagcgcgc ttcccatcaa
     1501 cggcgatgcg actgcttccg gcgccggcga agtccccaag ccgacgacct cgaccacgga
     1561 agagccggct cccccaacgc ggccagcgcc gacacgcgcc gcaactgcgc gtgcgggcgc
     1621 gaatgcggtc gcttctagca ccgccgttcc gacgcccgta ggcgctccaa agcccgacag
     1681 aactggaagc aaggtcgctc cagcaaagcg cgcccccaat ccttctccga agcgcgacga
     1741 caacgcatcc gggatctgac atgagaatcc gtacacgact attcgcggtc gccttcgcga
     1801 gctcgttgct aggtaccgca cccgccttag cgctgagcga cagtgacaaa gaagcgattc
     1861 gcgtcctttc gaaccaagcg gccgccgatt tcgaccaggg ccgctacgaa gccgctcgcg
     1921 acaaattcga acgagcgtat gcactagcca gtgttccgaa gcttgccgtg tggctagctc
     1981 gctctcaaga gaagctcggg cgttgggtcg aggcctatga gctctatcgc caagcggtga
     2041 gcttgcagcc gaacgaactc tggaaaggca ccgtccagca aaatgctcaa agtgatgccc
     2101 gcgccgcgat cgacgcgctc tacgctcgca tcccaaggct cacgctcagc gtcgaaggcg
     2161 cgccgctcgc agaagtgaaa gtgaccatcg acggagccga agtgccgagc ggactgctat
     2221 ccgtcgagcg cctgctcaac cccggagagc acgacgtcgt cgcccgcttt ggcgagcaat
     2281 ccctgaacga acacgtatcg ctgtcggaac gtgacgtgaa ggcgctcgcg ctacgcttta
     2341 cgtcgacgca acccacaccg gccgccgccg cgcctattgc cgcaaccgct gtttcctcat
     2401 cgccgctccc ggcgccacct gcgattgtcc cgccgcagac gcagacgcag actcagactc
     2461 cagcgccggg cgcgctcgca gggtcggcgg cgcgccgacc aggggctggg cagcgcacgg
     2521 ttggttgggt gagcgtcggt ctcggcgcgg ctggaattgg gctcggcgcc gtgacggggg
     2581 tgttgcttgc gggaaagtat tcggatctca cttcgaagtg cgaactccgc gtgtgtccgg
     2641 cagagcggaa gtccgacgta gaaagttacg atcgactgcg cacgctctcg agcgttggct
     2701 tcatcgcggg cggagcgctc acggcgctcg gtgttacgtt gctgctgacc gcgccgaaat
     2761 cggggggaac caccggcatc gagctcgtgg tttctgcacg cgacgcgact ttgctcgggg
     2821 tgttttgatg tccacgcgac gactctcgtt cgcggcggcg ctacttggtg tcggctgcgc
     2881 atttcccagc ttcgaagtga gagagcaatc cggcggtgct ggaggcgcgt ctcgcggcgg
     2941 gtccactgcg ggtggcgctg gcggcaagtt gggcgcaggc ggcgccccga gcggtggcgc
     3001 aactggcgcg cccggcggca agagcggcgg cgcgtcctct tcgcagggag gcgcgtcctc
     3061 ttcgcagggt ggcgcatcga cgagcggcgg cggccgcggc ggctcgtcga gcggaggtaa
     3121 gtcgaacgga ggtgcaacca gcggaggtgc gccgggtggc ggtgcgccgg gtggcggcac
     3181 gacgagcaac ggtggctctc acggtggagc accgacggcc ggagcaccag gcaattgcgg
     3241 tggcgcttcg agcaccgcac gcggaccgtt tacatgcggc tcgacgccag caacgtgcaa
     3301 ccagatctcg agcttcccgc tggacggagc gtggggcagc ggtgacttta cgggagggct
     3361 cgttgtttac gggaagggat tgagccgcga catgacgcaa accgaggtgc tccacataac
     3421 gggcaccgtc gacgactacg tgggattcgc attctggttt tcgacgtgca gcagcttgga
     3481 cacgttcaaa ggcgtgcaat tcaagctatc gaacatgctt ggcgcgggga aaaccgtgcg
     3541 cttccaactg cagaccaacg ccgacttccc ttggcaatcc gatcccaacg cgggcaaagg
     3601 cgcttgcact gccacgacgt gcgatggagt gtggggcgag tgc
//
LOCUS       DASTKS010000227         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_36347,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000227 DASTKS010000000
VERSION     DASTKS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36347"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1119
                     /locus_tag="VFQ35_04750"
     CDS             172..1119
                     /locus_tag="VFQ35_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HET9929970.1"
                     /translation="MNANTLPLGADEHLFFEMVTALVRSVQAGEPLRDAGYVLDGAAW
                     VESTREEALFWVDREHEPAFGVRGSLPAAIKQLLELYVPLCLGGGARRLTIAHLGQSL
                     DGRIATETGASRYVTGQENLIHVHRLRALCDAVLVGARTVAADDPQLTTRLVSGRSPV
                     RVVIDSQRRLSAESRIFHDGAAPTCVICARGASRRAPLPARVEVIELDSDAGAISPEN
                     VLGALHERGLRWVFLEGGGLTVSRFLLAGVLDRLHLTVAPIVLGAGVSALSLPGLRDV
                     TQALRPRTRRFFLGDDVLFDCALERGRTFGGARAASDGA"
     gene            1116..2120
                     /locus_tag="VFQ35_04755"
     CDS             1116..2120
                     /locus_tag="VFQ35_04755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9929971.1"
                     /translation="MKPWVFVVPDRSGHPSGGTRYNTALLRELGAQGAELVIVPSNEL
                     GPISASHAEWIWVDSLYLSLVAQLAARARGDWRARVGILLHYLPSLLSSGNAALSGAE
                     HEALQACDALVVTSEYMKAELSRRGYGAKPCAVVEPGCELTLAATAPDVTSLRALMVA
                     HVVENKGVYELLRALATELSGSDELSLEIAGSLEVAPEYSRRCRGLLDDAPLLARSVT
                     WLGTLDEDSTRRAYLRNNLFVSASRSESYGMALAEARTVGLPVIANAGGNVSALVNRR
                     SGGRLCANADEVAVACVELARSASARAEVTALARAEAIPPRPWARVAEEFTERVAQFR
                     "
     gene            complement(2127..>2558)
                     /locus_tag="VFQ35_04760"
     CDS             complement(2127..>2558)
                     /locus_tag="VFQ35_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9929972.1"
                     /translation="SSAFGPALLAILALDGLDGALARRLEQSSAFGAHFDMETDALLV
                     AIASLELWSRGLTGAWVLSSGALRYLYVVVLWLFPAPGGDAPRSRWGRYSFLILVLGL
                     SAPWLLGSPLGAPLAAVGCLVVVLSFARSFAYSYGLLRAAN"
BASE COUNT          411 a          824 c          887 g          436 t
ORIGIN      
        1 agcggttccg attcacgtga tcagccgccg cagcgatctc gttcagactt ggctcgagcg
       61 cggctatcgg cccaccgtgc gtccggatct cggctctccc gtcgaaccgc tcgcgagttt
      121 ccttccgacg ttgctcgaga gcctcaccat ccgaaagcag ggagcgccgg gatgaacgca
      181 aatacgctac cgctgggcgc ggacgagcac ctcttcttcg aaatggtaac ggcgttggtt
      241 cgctccgttc aggcgggtga gccgctgcgt gacgctggct acgtcctcga cggcgctgca
      301 tgggtcgagt cgacccgcga agaagcactg ttttgggtcg accgcgagca cgagccggcc
      361 ttcggtgttc gaggctcgtt gccggccgcg atcaagcagc tgctcgagct ctacgttcct
      421 ctgtgtttgg gagggggagc tcgtcggctc acgatcgcgc acttggggca gagcctcgat
      481 ggtcgcattg ccaccgaaac cggagcctct cgctatgtga cgggacagga gaacctcatc
      541 cacgttcacc gcttgcgggc cctttgcgac gccgtcctcg tcggtgctcg caccgtggcg
      601 gcggacgacc cccagctcac gactcggctc gtttctggac ggagcccagt gcgcgtcgtg
      661 atcgactcgc agcggcgcct ctcggcggaa agtcggatct ttcacgatgg cgcggcgccc
      721 acgtgcgtaa tttgcgcgcg cggcgcctcc cggcgagcgc ctttgcccgc gagggtcgag
      781 gtcatcgaac tcgactccga tgccggcgcg atctctcccg agaatgtcct cggcgctttg
      841 cacgagcgtg gcttgcgttg ggtgttcttg gaggggggag gcctgacggt gtcgcgcttc
      901 ttgctcgccg gcgtgctcga tcggttgcat ctgaccgtcg cgcccatcgt actcggcgcg
      961 ggcgtgtcgg ctctcagttt gccggggctg cgcgacgtca cccaggcgct ccgtccacgg
     1021 acgcgtcgct tcttcttggg cgacgatgtg ctcttcgatt gtgcactcga gcgggggcgg
     1081 acgtttggcg gcgctcgcgc ggcgagcgac ggcgcgtgaa gccctgggtc ttcgtagtcc
     1141 cggatcgttc cgggcacccg agcgggggaa cgcgctacaa caccgctctg cttcgagagc
     1201 taggagcgca gggagcggag ctcgtgattg tgccgtcgaa cgagctcggg ccgatcagcg
     1261 caagccacgc cgagtggatt tgggtcgaca gcctctatct ctctctcgtg gctcagctcg
     1321 cggctcgagc aagaggcgac tggcgcgctc gggtcgggat tctgttgcac tacctgccgt
     1381 cgttgctttc gtcgggtaac gcggcgcttt cgggcgcaga gcacgaggcg ctgcaggctt
     1441 gtgatgcgct ggtcgtgacg agcgagtaca tgaaggcgga actttcgcgg cgcggatatg
     1501 gcgccaagcc ttgcgccgta gtcgaacctg gatgcgagct cacgctcgcg gcaacggcgc
     1561 ccgacgtgac atcgttgcgg gcattgatgg tcgcgcacgt cgtagaaaat aagggcgtct
     1621 acgagctctt gcgcgcgctt gccaccgagc tctcggggag cgacgagctt tcgctcgaaa
     1681 tcgcgggctc gctcgaggta gcgccggagt attcgcggcg ctgcagggga ttgctcgacg
     1741 acgcgccgct gcttgcccgc tctgtcacgt ggctcggcac gctggatgag gactcgacga
     1801 ggcgcgccta cctacgcaac aacttgttcg tttccgcctc gaggtcggag tcttatggaa
     1861 tggcgctcgc cgaggcccgc acggtcggac ttccggtcat cgcgaacgcg ggcggaaacg
     1921 tcagcgcgct cgtgaaccgc cgctccggcg ggcgactctg cgcgaacgcg gacgaagtcg
     1981 cggtggcctg cgtcgaactc gcgcggagtg catcggcgag ggcggaggtg acggcgctgg
     2041 ctcgcgccga ggccattcct cctcggccct gggcgcgagt cgccgaagag ttcacggagc
     2101 gcgtcgcgca gtttcgttag gggcccttaa ttcgccgccc ggagcaatcc gtacgagtac
     2161 gcgaaagagc gggcgaacga caggacgacg accaagcagc ccacggccgc gagcggagcg
     2221 ccgagcgggg agccgagcag ccacggcgcg ctgagcccca gcacgagaat cagaaaagag
     2281 tagcgccccc agcgcgaacg cggcgcgtcg cctccgggcg ccggaaacaa ccaaagcacg
     2341 acgacgtaga ggtagcgcaa agcgccggac gacagcaccc aggcgccagt gagcccgcgc
     2401 gaccagagct cgagcgacgc gatggcaacg agcaaggcat cggtctccat gtcgaaatgc
     2461 gcgccaaagg cggagctctg ctcgagtcgc cgcgcgagcg caccatcgag gccatccaaa
     2521 gccaagatgg cgagcagcgc gggcccgaac gccgacga
//
LOCUS       DASTKS010000228         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_927832, whole genome shotgun sequence.
ACCESSION   DASTKS010000228 DASTKS010000000
VERSION     DASTKS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927832"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2050
                     /locus_tag="VFQ35_04765"
     CDS             <1..2050
                     /locus_tag="VFQ35_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9929973.1"
                     /translation="PEGTHRTFAGLGLHLAEGYGLTEASPVLTVAEGRPGAKPGHVGV
                     PIPGVEIRIESPNNEGVGEILARGPNVMLGYMGDPEATRGSITAEGWLRTGDLGKLDR
                     RGRLSIVGRAKDVIVASNGENVYPDDVEAKLGRVEHVAEFAIVGISDGRGGERVALAG
                     VPESESGLPRTERHARARRALEAEVNKLPQAMRPAVVTLFDSKLPRTATRKVKRAELR
                     RMLERTLDTSTPVGLASSQLDAAAVSVRAAVGAISRRDPARVQASQSLRGDLGFDSLM
                     LLELLVALENQVGGSVDAERLNACVTVADAEALVREAREHKRVASSTQSIEKKEVSFE
                     IPSELRAAAMEWMGRAQLGFYDKVLSTKVSGRAFIPHNRNTLVAANHASHLDMGLVKY
                     ALGRYGDGIVSLAAQDYFFEGPAWRRFYFENFTNLVPMTRSGALRQALRQAGDLIEQG
                     KTLLIFPEGTRGTDGAVHEFKPAVGYLALHHGIDVLPVYLGGTFAALPKGATVLRRRD
                     VEARIGPPLEIAELRRLTQGMAPADAARAVARLIERAVTALSKGEVLDTQKLSPADVR
                     EAPEPEDDSLENVFKELEGRFVRGSVANPLSFYFSLGDTERWTVRITENSCEVRPGKV
                     TGSADCVLKTSPQMFTRIVRERYTPSPAEFMAGAIKSNNIALLFTFQKAFQLDTPSAE
                     "
     gene            2047..3114
                     /locus_tag="VFQ35_04770"
     CDS             2047..3114
                     /locus_tag="VFQ35_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9929974.1"
                     /translation="MTRMENGTARGKKFWVGGATGFLGSHLVRVLRAAGHDVVAVSRG
                     GGDVDGVPVARVDVLDEDAVAESARGVDGAFLATGKVARDKDAAGELHRLHVVGTKTA
                     LAGLKRVGVPRVVVASTSGTIAVGTDPDRVYDESVSAPLEIIAPWPYYRSKLYAEREA
                     LAANEPGFEVVVVNPSLLLGPGDLRESSTADVRLFLSKSVPAIPAGGMAFVDVRDAAE
                     GMRLAFERGRPGERYLLNGKNLSVAAFFARLERISGVKGPSLPLPRSRPVAIGIGKLY
                     RRAVKAMGGEPPVDETTLEMGQYFWYCDSSKAERELGFSARDPGETLRDTIDDLVARH
                     VVFLKREARTGESAERGTIVS"
     gene            complement(3199..3774)
                     /locus_tag="VFQ35_04775"
     CDS             complement(3199..3774)
                     /locus_tag="VFQ35_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929975.1"
                     /translation="MSVVSAHLRVVRSAAEGDDEALAVLIRMHHDRVYRFGRRVCRDG
                     ADADDAVQEAFIKLAKRPDVIVDRGALSWLLSVVRRACLRLLRPFARRRRALGEPHEA
                     DDAPSTTLDPERAFERFELARRVHAAIAKLPQPCREVLILRDLEGLSGEETSSALGID
                     LAAMKSRLHRARTMMRESLREAPRANDRFTA"
     gene            complement(3771..4034)
                     /locus_tag="VFQ35_04780"
     CDS             complement(3771..4034)
                     /locus_tag="VFQ35_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9929976.1"
                     /translation="MNPSFESEPSQLERVLDMVKNVGTMDRLARGIGAFTLLLAAWFA
                     PLSTTMRFAVLAPMAVYMAFTALGGTCFGYRLLGKSSCGLSSR"
     gene            complement(4665..>4883)
                     /locus_tag="VFQ35_04785"
     CDS             complement(4665..>4883)
                     /locus_tag="VFQ35_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929977.1"
                     /translation="GAQNCRCSNSSGMWSCNNAPGGGGGAGGMGGGPRGGAGNVAGTS
                     GGGASNGGAGAGGASNGGASTAGVSGSN"
BASE COUNT          847 a         1688 c         1602 g          746 t
ORIGIN      
        1 gccagagggc acgcaccgca ccttcgctgg cctcggcctc cacttggcag agggctatgg
       61 tctcaccgaa gcctcgcccg tgctcaccgt tgccgagggt cgccccggcg cgaagcccgg
      121 tcacgtcggc gtgccgatcc cgggcgtcga gattcgcatc gagtccccca acaacgaagg
      181 cgtgggagag atcctagcgc gcgggccgaa cgtgatgctc ggctacatgg gcgacccgga
      241 agcgactcgc ggcagcatca cggccgaggg ctggctccgc accggcgacc tcggcaagct
      301 cgatcggcgc ggtcgcctct ccatcgtcgg gcgcgcgaaa gacgtgatcg tcgcctcgaa
      361 cggcgagaac gtgtaccccg acgacgtcga agcaaagctc ggccgcgtcg agcacgtcgc
      421 cgagttcgcc atcgtcggga tctccgacgg acgcggcggc gagcgcgtgg ctctcgccgg
      481 cgtgcccgag agcgaatccg ggctgcctcg caccgagcgc catgctcgcg cccggcgcgc
      541 gctcgaggcc gaggtgaaca agctcccgca agccatgcgg cccgcggtcg tgaccctgtt
      601 cgacagcaag ctgccgcgca ccgcgacgcg caaggtcaag cgcgcggagc ttcgacgcat
      661 gctggagcgc acgctcgaca ccagcactcc cgtggggctc gcttcgtcgc agctcgatgc
      721 agccgcggtt tcggtgcgcg cggcggtggg agccatcagc cggcgcgacc ccgcgcgcgt
      781 gcaggcgagc cagagcctgc gcggcgatct cggcttcgac tcgctgatgt tgctcgagct
      841 cctcgtggcg ctcgaaaatc aggtgggtgg cagcgtggac gccgagcggc tcaacgcctg
      901 cgtgacggtt gcagacgcag aggccctcgt gcgcgaagcc cgcgagcaca agcgcgtcgc
      961 ctcgagcacg cagagcatcg agaagaaaga ggtatcgttc gagatcccga gcgagctccg
     1021 cgccgcggcc atggagtgga tggggcgagc ccaactcggc ttctacgaca aggtgctctc
     1081 gaccaaggtc agcggccgag ctttcatccc tcacaatcgg aacacgctgg tcgccgccaa
     1141 ccacgcgagc cacctcgaca tgggactcgt gaagtacgcc ctcggccgct acggagacgg
     1201 catcgtgtct ctcgccgccc aggactactt cttcgagggt cctgcctggc gccgcttcta
     1261 tttcgagaac ttcacgaacc tggtgccgat gacgcggagc ggtgcgctgc ggcaagcatt
     1321 gcgccaagcg ggcgacctca tcgagcaggg caagacactg ctcatcttcc ccgagggcac
     1381 gcgcggcacc gacggcgccg tacacgagtt caagcctgcg gtcggctacc ttgcgctgca
     1441 tcacggcatc gacgtcttgc cggtgtacct cggcggtacg ttcgcggcgt tgccaaaggg
     1501 cgccaccgtg ctgcgccggc gcgacgtcga agctcgcatc ggtcctccgc tcgaaatcgc
     1561 ggagctacgg cgcctcactc aaggcatggc gcccgccgac gccgcacgcg cggtggcccg
     1621 gctcatcgag cgcgcggtga cggcgctcag taagggcgag gtgctggaca cccaaaagct
     1681 ctcaccggcc gacgtgcgcg aagcccccga gcccgaggac gattcgctcg aaaacgtctt
     1741 caaggagctc gaaggtcgtt tcgtgcgagg cagcgtcgcg aacccgctca gcttctactt
     1801 ctcgcttggc gatacggagc gttggacggt gcgtatcacc gagaattcct gcgaagtgcg
     1861 accgggcaag gtgacgggta gcgccgactg cgtgctgaag acctctccgc aaatgttcac
     1921 gcgtatcgtg cgagaacgct acacgccatc tccggccgag ttcatggcgg gcgcgatcaa
     1981 gtcgaacaac atcgcgctcc tcttcacctt ccaaaaggcc ttccagctcg ataccccgag
     2041 cgccgaatga cacgcatgga aaacggaacc gctcgaggta agaagttctg ggtggggggc
     2101 gcaaccggct tcctcggctc gcacctggtg cgcgtcttgc gcgccgcggg ccacgacgtc
     2161 gtggctgttt cgcgcggcgg cggcgacgtc gacggcgttc cagtggcgcg cgtggacgtg
     2221 ctcgacgaag acgccgtcgc cgagagcgcg cgcggtgtgg acggcgcctt cctcgcgacg
     2281 ggcaaggtcg cgcgcgacaa agacgcggcg ggagagctcc accgcctgca cgtggtgggc
     2341 accaaaaccg cgctcgccgg gttgaaacgc gtcggcgtac cgcgcgtcgt cgtcgccagc
     2401 acgagcggca ccatcgccgt cggcacggat ccggatcgcg tctacgacga gagcgtgagc
     2461 gcgccgctcg aaatcatcgc tccgtggccg tattatcgca gcaagctcta cgccgaacgc
     2521 gaagcgctcg ccgcgaacga gccgggcttc gaagtcgtcg tcgtgaatcc gagcttgctc
     2581 ctcggcccgg gtgacctccg cgagtcatcc acagccgacg tgcggctctt tctcagcaag
     2641 tcggtgccgg caatccccgc gggaggtatg gctttcgtcg acgtgcgaga cgccgccgaa
     2701 gggatgcgcc tcgcattcga gcgcgggcgc cccggtgaac gctatttgct gaacggaaag
     2761 aacttatcgg tcgcagcgtt cttcgcgcgc ctcgaacgga tcagcggcgt gaaaggtccg
     2821 agcctcccct tgccgcgctc gcgccccgtc gcgatcggca tcggaaagct ctatcggcgc
     2881 gcggtcaaag ccatgggtgg cgagccgccc gtcgacgaga ccacgctcga gatgggccag
     2941 tacttctggt actgcgactc cagcaaggca gagcgcgagc tcggcttctc ggcgcgcgac
     3001 ccgggcgaaa cgctccgcga cacgatcgac gatctcgtcg cacgccacgt cgtgttcctc
     3061 aaacgagaag cgcgtacagg cgaaagcgcc gagcgcggga ctatcgtttc gtaaatccgt
     3121 cggtcgtccc tcagcgctgg tcgagtgcct gcaagcggag acaccggagc aggcactcgg
     3181 actcgccgcg ggtcgcgact acgccgtgaa gcggtcgttg gcccgcggcg cttcgcgcaa
     3241 gctctcgcgc atcatggtgc gagcccggtg gagacgcgac ttcatcgctg cgaggtcgat
     3301 accgagcgcg ctgctcgtct cttcaccgga cagaccctcc agatcgcgca aaatgagcac
     3361 ttcgcgacac ggctgcggca gcttggcgat ggcggcatgt acccgacggg cgagctcgaa
     3421 gcgctcgaac gcgcgctcgg gatccagcgt cgtcgagggc gcgtcgtccg cctcgtgagg
     3481 ttcgccgaga gcgcgccgac gcctcgcgaa cgggcgcaac aagcgaaggc atgcccgacg
     3541 cacgaccgag agcaaccagg agagcgcccc tcgatcaacg atgacatccg ggcgcttggc
     3601 gagcttgatg aacgcctctt gcacggcgtc gtcggcgtcc gcgccatctc gacagacccg
     3661 gcgaccaaac cggtacacgc ggtcgtggtg cattcgaatc aggaccgcga gcgcctcgtc
     3721 gtcgccctcg gccgccgacc gaaccactcg caggtgcgcc gagacgacgc tcatcgggac
     3781 gacagcccgc agctcgactt gccgagaagg cgatagccga agcaggttcc tccaagagcc
     3841 gtaaacgcca tgtaaacggc catcggcgcc agtacagcga agcgcatcgt ggtcgacaag
     3901 ggggcgaacc aggccgcaag cagcaaagtg aatgcaccaa tgcctcgagc caggcgatcc
     3961 atcgttccga cgtttttgac catgtccaat accctttcga gctgagaagg ctcggactcg
     4021 aaggaaggat tcatggttca gccacttttt tcggcagggg ctggttacag cttttcagga
     4081 cgacaccgcg ttgacatcct gcgtcgtacg ccacgagcag gtggcgcgca cgctcgagct
     4141 cgggatcgtc gcaccgaaag caagtgaagc ccggaccgat tccaaaatcg gcgcagtcga
     4201 cggggcgcca cgacatcgga gaggtttcgg ggcaggcacc ggcgaaagac ggcatctcgc
     4261 ccgcgctgcg tccttccctc gcgaggaact ttcggcacac gtcaggcttg ggcttgccta
     4321 ccgcgcggaa ttgatagcgg cactcctgaa agctcgtgtc ctccccttcg cagctctctg
     4381 tctcgagaag cgcgcttcga cactgttccg gctcgctgag acgcactcgc gaaaacggcc
     4441 cgacctgcat gccgtccctg cgcagtagcc accagccggc ggcgacgagg gcgccaaagc
     4501 ccccgattag accgagcagt gctcgccgtc gccatccgtg tccacgcacg ctggcaagct
     4561 tagcgtggct cggcaccggc gggcccgcag cgctcggcac cgacgtgcgc aaggcgcgcc
     4621 gcgagccggc tgacgtttgg ctcgcggcgc tcgcgcggta ttactcagtt cgagccgctg
     4681 actcccgcgg tgctcgcgcc gccgttgctg gcaccaccgg cgcccgctcc gccgttgctg
     4741 gcgccgccgc cgctcgttcc agcgacgttg ccggctccgc cgcgcggacc gcctcccatt
     4801 ccgccggcac caccaccacc accgggggca ttgttgcagg accacatgcc gctcgagttg
     4861 ctgcaacggc agttctgggc gcc
//
LOCUS       DASTKS010000229         8596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_704528, whole genome shotgun sequence.
ACCESSION   DASTKS010000229 DASTKS010000000
VERSION     DASTKS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704528"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(686..1345)
                     /locus_tag="VFQ35_04790"
     CDS             complement(686..1345)
                     /locus_tag="VFQ35_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET9929978.1"
                     /translation="MSSKRTSRMPARPYFMGLVVSLLGCATTPLDVLEAASTNGGDTS
                     NGGANEGGNGGARGGNGGSGGGATCAPAPAALAGTYRLRAATSGKCVGVGMSTLVGSL
                     PARLTVMVNDCSARGEIFQLIADSGFGSFQLRSATTGYNLDIEMLGSSDGTRVILFSM
                     NGLANQRFTFQTRRERVFALIPGNADSSCVTEVTPQPEIFACKNDLQTQEWELVPVNC
                     D"
     gene            1920..2537
                     /locus_tag="VFQ35_04795"
     CDS             1920..2537
                     /locus_tag="VFQ35_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9929979.1"
                     /translation="MPILVSPKSDDPLLESEELHSGVSTRGSDWQREAFEQYHGLVHG
                     LVLKCLGPNAETMDLVSEVFVKFFENAHRIRSQSAIRSYLVSITMNAIRGEVRRRKRR
                     ELLLKLSGLIPDLEHEPGQDDPKAKAALIQLSRILDELGVNARMAFVLHSLEGLHMAE
                     IAEALKVSESTAKRWVRRANEHVLKRVSRNALLADYIQDRRPNRA"
     gene            2530..3825
                     /locus_tag="VFQ35_04800"
     CDS             2530..3825
                     /locus_tag="VFQ35_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9929980.1"
                     /translation="MRSRRDSLTSSDVGSAFRALADEHAEKRANSERWATWQLVQRRL
                     VASEGLGTEQSSRRMLWPAVGFSIAVAAGVAFAVLPQGLRYEVHGADVAAGWIQSKSS
                     AGSVDFSDGSQVAVQPGSSLAIEVVGAHAARTRLAQGDLDVSVKHEDNTDYRFLAGPY
                     QVHVVGTKFHLVWHPEQQLFSVQMREGRVQVTGPNGLDRAVVAGETLRISGAEEQAAL
                     ARQTENAPPRVPQLPSAQKAAEQPVAELAPLQRNAGATGTALGSAARSNVVASATRVE
                     PTPAWSVLVAKGQFTDVVKAAEAVGTERALRERDAADLGALAHAAHYTGRSALAISAW
                     SSLRERFAGQKSARQAAFFLGRVFDQQGRRAEALRWLNTYLSEAPADVYASEALGRKL
                     SLVNHLEGALAARNVAREYTKRFPHGAYAQTARAILDED"
     gene            3829..4905
                     /locus_tag="VFQ35_04805"
     CDS             3829..4905
                     /locus_tag="VFQ35_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929981.1"
                     /translation="MAKARRLRSRPLLVLASALVACGVTALPASVHAEPRVLIEVPDT
                     SDPVLTEALNRVRGELSAVGLDADVHPFHGEAPEPVLESGVEGALSLERENSIIRIRA
                     WGPLSPEPVVQELDTRASDVTAEVVSVRAVEALRAVTVAFRKREPKPVEPPKPPPRPE
                     PPPPPPPKPVPRKVARPALAPRGEHGALTFTLAPNSFYDVDSRTLGVGGEVGAYYGRF
                     PCFVGPQLTSTLYRPALRVDAGTVDTKRLSAALRAGCSLELGGAFELWVSLGGGLARY
                     DVEGHPASGFRGNRVQHSSPFLTSGVGASLWFSTHVGVFARVDASVATDTAAIRVLGA
                     EIASLEKPLLWGAVGIALQVPSVL"
     gene            4974..5684
                     /locus_tag="VFQ35_04810"
     CDS             4974..5684
                     /locus_tag="VFQ35_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929982.1"
                     /translation="MRLWMLVLLVFVGCSNGRLDAFTREGEGAGGAPSGGSASGGAAR
                     GGAPSTGGTGMARGGTSNSGGGMTGPLVLDDFEDGDNQTLPPMGWWYVETDMTPNAIM
                     MDTKAPNAHGGARSLRVYGSGCRDWCFLGVDLPGEEPFDARAYSRISFWARAEPSSVV
                     HTLSVDVLDSPNGVHFRNDIDLSAQWGNYTVNFAELVPTESSSTAVLDRSHLIQVEFW
                     IFSPEAFDFYIDDVVFLP"
     gene            complement(5734..6153)
                     /locus_tag="VFQ35_04815"
     CDS             complement(5734..6153)
                     /locus_tag="VFQ35_04815"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9929983.1"
                     /translation="MRKANSAQQQAAPAPAHAPEPDHGGAHRRNHPRYSVDLDVSLGS
                     EHNFYSGFAENLSVGGVFVATHQVRPVGEKVEVSIHLPDGTEVRAVGEVRWTRLFNAE
                     SDMPPGMGLRFTELNAGADTAIERFLRQREPLFYDDE"
     gene            complement(6336..8129)
                     /locus_tag="VFQ35_04820"
     CDS             complement(6336..8129)
                     /locus_tag="VFQ35_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929984.1"
                     /translation="MRQLWPLGLVLLALGCGSGDRAPHLIDLQEVQPRHLEVGDRMRL
                     TGSGFPEGRPATVTFRGETHRAGEVPRTSTSVSADAEQVAPHALELPISAELAAKLCG
                     AGAPRHATFRGDVEVSFSPQTASGLPVIGRLDGVVIDFVPALRDDAELGALRGDGQRF
                     ARFLGVGVIAGASGLQVTQVESQGRAARANLTVGDVIQELDGIVVRGVADFIPPPNAK
                     SSLLVVRRGPDSMALRVDSSGFRYSAPETLLPALFVALLLLGAFGLAASPIGRWLSLF
                     ERRVGERLRASRSNGSQPRIRDALGSLLAEQLPESFLPYLSIVGFCALFSMLSLSKSV
                     IWIELDVLLLPAASLSALAVSAFLAGRGERWTLRGALQELGAVLLFNAPIALLILVTG
                     WWAGSLRVSDVVGLQGPWPWQWAVFRNPVLGVVALMSILSRVASARDRSVGRALQSNA
                     RQRALSLTAFVHTLCVCGIIALVGLGGPALPFTQGSLWVLGLSVVLTLAKASSLVVIV
                     ALLRWTLGVTDIVSGARGALFGLVLPSVAAVAAMAGLSALGAGPVAGTLSQALPASLF
                     SACALVSAWVAARVARGARNPTAELRIQSWL"
     gene            8287..>8596
                     /locus_tag="VFQ35_04825"
     CDS             8287..>8596
                     /locus_tag="VFQ35_04825"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HET9929985.1"
                     /translation="MADLENLIERLRAVQTEVLRLAPFRDIGLVPNPGASAQAIDAAE
                     ARLGIPLPPSYRAFLARHDGWPRCFDGATLLGTACLGNRTYAEFAQAAFAAAETPEPL
                     G"
BASE COUNT         1539 a         2922 c         2773 g         1362 t
ORIGIN      
        1 ccagatgtcg acgaattgcc cgcaacgggg gccccgcctc cggccgcggc gttcgagccg
       61 ccggaaccgt cgtcgttatt gccgttgccg ggactcgtca cggtgccctg acatccgagc
      121 agcagacctc cgacaaccca gtactgcgcg atgacgctgg agcgcttcat gcctctacct
      181 caagtcgtct gagagcccct tcagaaggcc tcgcagcgca ctttcgcgcg gcgagataaa
      241 aatccggcgg cgctgctcac cgagcgcaac tctttgcaac tttgcttgtc catgttttag
      301 cgagcaagtc accgcctggt agacgctgag cccgcccgcg agaagcacgg gtcaaaaaag
      361 tcttcacggg tgccacagag tgacccacgc acttccgcgc acctactcga tgcgcgaatt
      421 cgcgcaattc tagctcgaat tccgagcaaa aagacgcgtc ccccgagggt atcgccccca
      481 cgagtcgcga aaacgaaatt ccgcgaacga ggtgaaacac aattcgtgcc ttcggctact
      541 cgatcgaatt ggaagatcga gattaggagt gcgcgcaaag ccacacgcgt ggtcgcggcg
      601 gagctctgcg tgacgcctca cgcatcgacg cagctacggg ctcagttgaa tggacgcgat
      661 cctcggttcc gtagcgaccg atgccctaat cgcaattcac aggaacgagt tcccactctt
      721 gggtttgtag gtcgttcttg caagcgaaga tctccggttg aggagtcacc tccgtaacgc
      781 aactcgagtc tgcgttaccc gggatcagcg cgaacacgcg ctcgcgtctc gtctggaacg
      841 tgaagcgctg gttcgctaac ccgttcatcg aaaacaggat cacgcgcgtc ccgtccgagc
      901 tccccaacat ctcgatgtcc aagttgtagc cggtcgtcgc gctgcggagc tgaaatgagc
      961 caaagccgct atcggcaatg agctgaaaaa tctcgccccg cgcgctgcaa tcgttgacca
     1021 tcacggtaag gcgagccggc aagcttccga cgagcgtcga catgccgacc ccaacgcatt
     1081 tcccgctcgt agcagcgcgc aagcgatagg tcccggcgag cgccgctggc gctggcgcgc
     1141 acgtcgcgcc cccgcccgaa ccgccgttgc cgccgcgagc gccgccgttc cctccctcgt
     1201 tcgcgccccc gttgctcgtg tcgcctccat tcgtcgaggc ggcctcgagc acgtcgagcg
     1261 gcgtcgtcgc gcaaccgagc aacgaaacca cgagtcccat gaaatacggg cgcgcaggca
     1321 ttcggctcgt tcgcttcgaa gacatcggcg gatcgctttc tcacgctagc gctctctgct
     1381 tcgattgcaa aacactcagc gtccgctcga ggagaacacc tgcgagatcc ctcgtctggc
     1441 tgcgcgagat ggttggttcg agccgaagcg tcgattggag gcaggggcgt cgcctcgatt
     1501 cgctgcgaag gagcgatcgg atgcttggtt cgtcgtagcg ttcgggcccg gccgcaaccg
     1561 cggcaaccag ccgagtcgtg aaggatcctt cagggcgaac acgcaagacg ccggtcattt
     1621 tcctcgcgtg cggagtttct cgatctggag cagacatttc gctcggacgc tcgcgaacga
     1681 tcttttttgc gacgcgaaag cgacctccga cgtctgctcg ggtgttctcc accgaccgct
     1741 ccatccgctc gcgcggggga tcacgggttt acgctccggc acgctcacga gcggcgctcg
     1801 aggcacgaaa ccccggtcgc cccaaacgcc acaccgatca acccggacca cgactcgaag
     1861 agaacgcccg acgcgatctg agttcgccag agagagaaag ctgctaagct gcgacccgag
     1921 tgccgatcct cgtttccccc aaatccgacg acccgctgtt ggagagcgag gagcttcact
     1981 ctggcgtctc cacacgcggc tccgattggc aacgcgaggc cttcgagcag taccacggcc
     2041 tcgtacacgg cctcgtgttg aagtgcctgg gaccgaacgc cgaaacgatg gacctcgtca
     2101 gcgaggtctt cgtgaagttt ttcgagaacg cacatcgcat ccgctcgcag agtgcgattc
     2161 gcagttacct ggtgtcgatc accatgaacg cgattcgcgg cgaggtgcgc cgtcgcaagc
     2221 gccgcgagct cttgctcaag ctatccgggc tcattccgga tctcgagcac gagccgggac
     2281 aagacgatcc caaagccaag gccgcgctca ttcagctgag ccgcatcctc gacgagctcg
     2341 gggtgaacgc gcgcatggcg ttcgtgctcc acagcctcga aggcttgcac atggccgaga
     2401 tcgctgaagc gctcaaggtt tcggagtcga ccgccaagcg ctgggtgcgg cgagcgaacg
     2461 aacacgtctt gaagcgcgtc agtcgcaacg cgctgctcgc tgattacatc caagaccgga
     2521 ggccgaatcg tgcgtagccg gcgcgacagc ttgacttctt ccgacgtagg ttcggcgttc
     2581 cgcgcgctcg cggacgaaca cgcagagaag cgcgcaaact ccgagcgctg ggctacctgg
     2641 cagctggttc agcggcggct cgtcgcgagc gaggggctcg gcaccgagca atcctcgcgt
     2701 cgcatgctgt ggcccgccgt cggcttctcc atcgcagtcg ccgctggcgt cgcgttcgcg
     2761 gtgttaccgc agggcctccg gtacgaggtg cacggcgccg acgtcgccgc cggttggatt
     2821 caatcgaaga gcagcgccgg cagcgtcgac ttctccgacg gcagccaagt cgccgttcag
     2881 ccgggcagct cgctcgctat cgaagtcgtt ggcgcgcacg ctgcgcgcac ccgcttggcg
     2941 caaggcgacc tcgacgtgag cgtcaagcac gaggacaaca cggactaccg cttcttggcg
     3001 gggccgtacc aagtgcacgt agtcgggacg aagttccatc tcgtctggca cccggagcag
     3061 cagctcttct ccgtgcaaat gcgagagggg cgagtgcaag tgacgggccc gaacggcctc
     3121 gatcgggccg tggtcgccgg cgaaacgttg cggatctccg gcgcggaaga gcaagctgcg
     3181 ctcgcccgac agaccgaaaa tgcgccgccc cgcgtgccgc agctacccag cgcgcaaaag
     3241 gccgccgagc agccggtcgc ggaactcgcg ccgctgcagc gcaacgctgg cgcgacaggc
     3301 actgcgctcg gttcggcagc gcgctcgaac gtcgttgcga gcgccacgcg cgtcgagccg
     3361 acgccggcgt ggtcggtgct cgtcgccaaa ggtcagttca ccgacgtcgt caaagctgcc
     3421 gaggcagtgg ggaccgagcg agcgctgagg gagcgcgatg ccgccgacct cggcgcgctg
     3481 gcccacgccg cgcactatac cggccgcagc gctctcgcga tcagcgcgtg gagctcgctc
     3541 cgagaacgct ttgctgggca gaagtcggcg cgccaagcag ccttctttct cggtcgcgtt
     3601 ttcgaccagc aaggtcggcg ggccgaagcg ctgcgttggc tcaacaccta cttgagcgaa
     3661 gcgccggcag acgtctacgc ttccgaggcc ctcggccgta agctctcgct cgtcaaccac
     3721 ctcgaggggg ctcttgcagc gcggaacgtc gcgcgcgagt atacgaagcg attcccccat
     3781 ggggcgtacg cgcaaacagc gcgagcgatt ctcgacgaag attgagcaat ggcgaaagcg
     3841 agacgcctgc ggagccggcc tctgctggtg ctcgcgagtg ccctcgttgc gtgcggagtc
     3901 accgcgctcc ccgcttcggt gcacgccgag ccgcgggtct tgatcgaggt tcctgataca
     3961 tccgacccgg ttctgaccga ggcgctcaat cgcgtgcgcg gtgagctctc ggcggtcggg
     4021 ctcgacgctg acgtacaccc ctttcatggc gaagcgccag agcccgtgct cgagtcgggc
     4081 gtcgaagggg cgctgtctct cgagcgcgaa aacagcatca ttcgcatccg cgcctggggt
     4141 ccgctctcgc cggagccggt cgttcaagag ctcgatacga gggcgtcgga cgtcacggcc
     4201 gaggtcgttt cggtgcgcgc ggtcgaagct ttgcgcgccg ttacggtagc gtttcgaaag
     4261 cgcgagccga agccagtgga gccgcccaag ccgccgcctc gcccggaacc gccgcctcca
     4321 ccgcctccca aaccggttcc acgaaaagtg gcgcgaccag cgctcgcacc gcgcggagaa
     4381 cacggcgcgc tcacgttcac gctcgccccg aactcgttct acgacgtcga ctcgcgaacc
     4441 ctcggagtgg gcggggaggt cggcgcttac tacggtcgct tcccgtgctt cgtcggcccg
     4501 cagctcacga gcaccctgta ccgtccagcg ctccgcgtcg atgccggcac cgtcgacacg
     4561 aagcgcctca gcgcggcgct gcgcgcaggt tgctcgttgg agctcggcgg cgccttcgag
     4621 ttgtgggtct cgctcggcgg tgggctcgcg cgttacgacg tcgaggggca tccggcgagc
     4681 ggcttccggg gaaaccgcgt gcagcactcc tcgcctttct tgacgagcgg ggtcggcgca
     4741 agcctttggt tctccactca cgtcggcgtg tttgcgcgcg tcgacgcgag cgtcgcgacc
     4801 gacaccgccg ccatccgcgt gttgggcgct gagatagcgt ccctggagaa gccgctcttg
     4861 tggggggccg tcgggatcgc gctgcaggtg ccctcggtct tgtgatcgca gcccgaaccg
     4921 tcgtcgaaac ttgcgagcgg gccgcgggtt gatacgttga gccgctcgcg ggaatgcgtc
     4981 tctggatgtt ggtgctgctc gtcttcgtcg gctgctcgaa cggccgcctc gacgcgttca
     5041 cgcgcgaagg tgagggcgcc ggaggtgctc cgagcggagg ctcggcgagc gggggagccg
     5101 cacgcggcgg cgcacccagc actggcggca ccgggatggc tcgcggcggc acgagcaact
     5161 cgggtggcgg catgaccgga ccgctcgtgc tcgacgattt cgaggacggc gacaaccaaa
     5221 ctcttccgcc gatgggctgg tggtacgtcg agaccgacat gaccccgaac gcgatcatga
     5281 tggacacgaa agcgcccaac gcgcacggcg gcgctcgctc gcttcgtgtc tacggcagcg
     5341 gctgtcggga ctggtgcttt ctcggcgtcg acttgccggg agaggaaccc ttcgatgctc
     5401 gcgcgtactc gcgcatcagc ttctgggcgc gcgccgaacc gagctcagtg gtgcatacgc
     5461 tgagtgtcga cgtgctcgac agccccaacg gcgttcactt ccggaacgac atcgatctgt
     5521 cggcgcagtg gggtaactac acggtcaact tcgcggagct cgtcccgacc gagagcagct
     5581 ccacggcggt gctcgaccgg agccacctga tccaagtcga gttctggata ttctcgcccg
     5641 aagcgttcga cttttacatc gacgacgtgg tgttcctacc gtagcgggcg cgtgctcggc
     5701 tcagacgcca gcctgtgggc gccttttcag ccctcattcg tcgtcgtaaa acaacggctc
     5761 gcgctggcgc aaaaatcgct cgatggccgt gtctgcgccc gcgttcagct ccgtaaaacg
     5821 caaccccatg cccggcggca tgtcgctctc ggcgttgaac aaccgcgtcc aacgcacctc
     5881 gccgaccgcg cgcacttcgg tcccatccgg taagtgaatg gagacctcaa ccttttcgcc
     5941 gactgggcgc acctggtgcg tcgcgacgaa tactccaccg accgaaaggt tctcggcgaa
     6001 acccgagtag aagttgtgct cgctgccgag gctcacatcg aggtcgacag aatagcgcgg
     6061 gtgattgcgg cgatgcgctc ccccgtgatc cggttccggt gcgtgcgccg gagcgggggc
     6121 agcttgctgc tgggcgctgt tggctttcct catggctaga cgcgacggct cgacgcaatg
     6181 gctaaagacg caaccgcgga gtcggcctcg aagagtcaaa cgaccgtcgc cggatcgggt
     6241 ttagccagcg ggagtacgaa aggagacgcc ctcgccccgg cgtccgagta accacggcga
     6301 actctgccag tttacggaag cttcgtacgt tcgctctaca accacgactg aattctgagc
     6361 tcggcggtgg ggttgcgcgc gccgcgggcg acacgtgctg cgacccacgc cgacacgagc
     6421 gcgcaagcgc tgaacaggct tgcgggtagg gcctgcgaca acgttcccgc cacaggccct
     6481 gctccgagag cgctgaggcc ggccatggcc gcgaccgcag ccacgctggg caagacgagc
     6541 ccgaacagtg ccccgcgcgc tccgctgacg atgtcggtga cgccgagggt ccaacgcaag
     6601 agcgcgacaa tcaccaccag cgatgacgcc ttcgccaatg tcagcaccac cgacaggccg
     6661 agcacccaca gcgacccctg cgtgaaagga agcgcggggc ccccgagccc gacgagcgcg
     6721 atgatgccgc aaacgcacag cgtatgaacg aaggcggtga gcgacagcgc gcgttgcctc
     6781 gcgttcgact gaagggcgcg cccgacggag cgatctcggg cgctcgcgac gcgggacaag
     6841 atcgacatca gcgcgacgac gccgagcacg gggtttcgaa acaccgccca ctgccacggc
     6901 cacggcccct ggagacccac gacgtccgac actcgcaagc tgcccgccca ccagcccgtg
     6961 acgaggatga gcaacgcgat cggcgcgttg aacaggagca ccgccccgag ctcttgcagc
     7021 gcgccgcgca gagtccagcg ctcaccgcga ccggcgagaa acgcgctcac cgcgagggca
     7081 gacagcgaag ccgcaggcaa cagcaacacg tcgagctcga tccaaatcac gctcttcgaa
     7141 agcgacaaca tcgaaaacag cgcgcagaag ccgacgatcg aaaggtaggg caagaaggac
     7201 tcgggcagct gttccgccag gagagatccg agcgcgtcgc gtattcgcgg ctgcgacccg
     7261 ttcgagcggc tcgcgcgcaa acgctcaccg acgcggcgct cgaatagaga gagccaacga
     7321 ccgatgggtg acgccgccag cccgaaggcg ccgagcaaga ggagggcgac gaacagagcc
     7381 ggaagcagag tctccggcgc gctgtagcgg aagccgctcg agtcgacgcg gagcgccatc
     7441 gagtcgggac ctcttcgaac cacgagaaga gagctcttcg cgttcggcgg tggaatgaag
     7501 tcggcgacgc cccgcacgac gatcccgtcg agctcctgga tcacgtcgcc caccgtgagg
     7561 ttcgcgcgcg cagcgcggcc ttgcgactcg acttgcgtca cctgcaagcc cgaggcgccc
     7621 gcgatcacgc ccacccccag gaaccgcgca aaccgctggc cgtcgccgcg cagcgcgccg
     7681 agctcggcgt cgtcgcgcaa cgcgggaacg aagtcgatga cgacgccgtc gaggcgcccg
     7741 atcacgggaa ggccgctcgc cgtttgcggc gagaacgaaa cctcgacgtc accgcgaaat
     7801 gtggcgtggc gcggagctcc ggcgccgcag agcttcgcgg cgagctctgc gctgatcggc
     7861 agctcgagcg cgtgcggcgc cacctgctct gcgtcagcgc tcacgctggt ggaggtgcgc
     7921 ggcacctctc cggcgcggtg cgtctccccg cgaaacgtca ccgtcgcggg gcgcccctcg
     7981 ggaaaccccg agccggtgag gcgcatccga tcgccgacct cgaggtggcg cggctgcacc
     8041 tcttgcaggt cgatcagatg cggagcccgg tcacccgagc cgcaacccaa cgcaagcaga
     8101 acgagcccga gtggccatag ctgtcgcacg cttcgcgtat acgcccatcg cggaaaacgg
     8161 cccaaaaagg cgccggttgc ggctcatccc gcgccgtcgc gggcgtcccc ggggcgcgaa
     8221 cggttcgcaa agaattgggc cgttctcggc gcgctgattt aggtcctggg gcaaccggag
     8281 gcacccatgg ccgacctcga gaacttgatc gaacgcttgc gcgccgtgca aacagaagtg
     8341 ctcaggctcg cacctttccg cgacatcggc ctcgttccga atccgggcgc gtccgcccag
     8401 gccatcgacg cggccgaagc acggctcggg atccccctcc ctccttcgta ccgagccttc
     8461 ttggcgcgcc acgacggttg gccacgctgc ttcgacggcg ccacgctgct cggtacggct
     8521 tgtttgggca atcggaccta cgccgagttc gcacaggcgg cgttcgcggc ggcggagaca
     8581 ccggagccgc tcggtc
//
LOCUS       DASTKS010000230         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_928160, whole genome shotgun sequence.
ACCESSION   DASTKS010000230 DASTKS010000000
VERSION     DASTKS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928160"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFQ35_04830"
     CDS             <1..849
                     /locus_tag="VFQ35_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9929986.1"
                     /translation="GRITEGTGRIKRIVQVDQKPIGRTPRSNLATYTGLFDEVRRLFA
                     STKAARARKYDAGRFSFNVAKGRCDTCQGEGFVSVELLFLPSVYAPCSACQGTRYNEK
                     TLEIKYRDKNIAEVLGMNVDAAFEFFADDASLRRSLDVLREVGLGYLRLGQPATELSG
                     GEAQRIKLATELQRSQRANTLYVLDEPTTGLHPADVERLMLQLSKLVDSGATVIVVEH
                     DMRVVAGSDFVIDMGPGAGDEGGRLVACGSPAEIARNPESRTAPYLARWLQGRDAGGA
                     RRASGA"
     gene            complement(939..1082)
                     /locus_tag="VFQ35_04835"
     CDS             complement(939..1082)
                     /locus_tag="VFQ35_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929987.1"
                     /translation="MSSKNDGSEVRQEYLPVSRGRKLVDTIVWVVIPLGFAVVLAVKY
                     WPH"
     gene            complement(1158..1571)
                     /locus_tag="VFQ35_04840"
     CDS             complement(1158..1571)
                     /locus_tag="VFQ35_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929988.1"
                     /translation="MTRTRPKRLKAREARLLSLLLVWAATALLITLEGAGATTRSATA
                     STRASQQDERTLQKPALFDAVALLAGHDELSEPQAGARSRSASRPQPHFAHERTELFA
                     AFGSDRLARTTPMPTVSHAVVFRKRIPRFSSDEPP"
     gene            complement(1568..>3119)
                     /locus_tag="VFQ35_04845"
     CDS             complement(1568..>3119)
                     /locus_tag="VFQ35_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9929989.1"
                     /translation="TMAATSGKLALFLAGLVVVGLLVVPRGVRAVLRLQRPETTLVAA
                     VGFCFAVSLLAHELGYSVALGAFIAGSLIAESGEHETIEHLVQPVRDMFAAIFFVSVG
                     VLIDPALIAQHWLAVLVLTVVVIVGKIVGVGVGAFLTGNSVRTSLRSGMSLAQIGEFS
                     FIIASLGLSLKATGEFLYPVAVAVSAITTLTTPWLIRASDPVANFVDRKMPRPIQTFV
                     TLYATWLEQLRSGPRSNTRAADTRRLILWLALDAALLVGLAIGFSLALPRLSSYVEHR
                     FGMGTRLVQGLRLGGALILTLPLSAAIVGVARRLAISIASAALPEPEEGSVDLSAAPR
                     RAFVATLQIAIVLLTGVPILVVTQPVLGGAYGAFLLAVLLLWLGVSFWRGATNLHGHV
                     QAGARALLEALMSQARQGPSDEPAPPGSERSQLEQLKTVLTGLGTPVAVKLDEQSRSV
                     GKTLAQLNLRGVTGATVLAITRPGAGIVIPTAREVLHAGDLLALTGTNDAIGAAKLRL
                     TAGGEADE"
BASE COUNT          571 a         1020 c         1044 g          484 t
ORIGIN      
        1 ggacgcatta ccgaaggcac tgggcgtatc aagcgcatcg tgcaagtcga tcagaaaccc
       61 atcgggcgca cgccgcggtc gaatcttgcg acctacaccg gcctgttcga cgaagtacgc
      121 cggcttttcg cgagcaccaa ggcggcacga gcgcgcaagt acgacgccgg gcgcttctcc
      181 ttcaacgtcg cgaaggggcg ctgcgacacg tgccagggcg aaggcttcgt gtcggtggag
      241 ctcttgttct tgccgagtgt ctacgccccc tgcagcgctt gtcagggcac tcgttacaac
      301 gagaagacgc tcgagataaa atatcgcgac aagaacatcg cggaggtgct cggcatgaac
      361 gtcgacgcgg cgttcgagtt cttcgccgac gacgcttcgc tgcgtcggtc gctcgacgtg
      421 ctacgcgagg tcgggctcgg ctacttgcgg ctcggacaac cggcgaccga gctgtccggc
      481 ggcgaagcgc agcgcatcaa gcttgcgacc gagctccagc gaagtcagcg cgcgaacacg
      541 ctgtacgtgc tcgatgagcc gaccaccggt ctgcaccccg ccgacgtcga acgcttgatg
      601 ctgcagctat cgaagctcgt cgactctggg gcgaccgtga tcgtcgtcga acacgacatg
      661 cgcgtcgttg ctggcagcga cttcgtcatc gacatggggc ctggggctgg tgacgagggc
      721 gggagactcg tggcctgcgg cagcccagcc gaaatcgcgc gaaaccccga gagccgtacc
      781 gctccgtacc tcgcgcgttg gctccaaggt agagatgcgg gaggagctcg acgcgctagc
      841 ggagcctgag ccggacggcg cgggtcatcg agcgctgcgg aagcttcccg atgcaaatgg
      901 cgggcgagat cgtccaaacg cgatctcgcc cgttcggttc agtgtggcca gtacttcacg
      961 gctaacacga ccgcgaagcc cagcgggatc accacccaaa cgatggtgtc tacgagcttg
     1021 cgtccgcgtg aaaccggtag gtactcctga cgcacctctg agccgtcgtt cttcgagctc
     1081 atttcggctc tcctcgaggc tcgtcgaaga gcctcggcaa aatggagttt ggttcacgtg
     1141 catcgtgccg acggagatca gggcggttcg tcggagctga accgtggaat gcgtttgcgg
     1201 aagacgaccg catgcgagac ggtcggcatc ggagtcgttc gcgcgagccg gtcagagccg
     1261 aacgccgcga agagctcggt gcgctcgtga gcgaaatgcg gctgcgggcg cgacgcactg
     1321 cgggagcgcg ccccggcttg cggctcgctg agctcgtcgt gccccgccag caacgctact
     1381 gcatcgaaaa gcgccggctt ttgcagcgtg cgttcgtcct gctgcgacgc tctggtcgaa
     1441 gcggtcgcgc tgcgagtcgt ggcgccggcc ccttcgagcg tgatgagcag agccgtggct
     1501 gcccaaacca gcagcaggga caagaggcgc gcctcgcgcg ctttcaggcg ctttgggcgg
     1561 gtccgcgtca ttcgtcggct tcgccgcccg ccgtgagcct gagcttggcg gctccgatcg
     1621 cgtcgttagt gccagtgagc gcgagtagat ctcccgcgtg gagcacttct ctcgcggtgg
     1681 gaatgacgat ccctgcgccg ggccgcgtga ttgcgagcac ggtcgctccg gtgacgccgc
     1741 ggaggttcag ctgagcgagc gtttttccga cgctgcggct ctgctcatcg agcttcaccg
     1801 cgactggcgt gccgagcccg gtgagcacgg ttttcagctg ctcgagctgc gagcgttcgc
     1861 ttccgggcgg cgccggctcg tcgctcggac cttgccgcgc ttggctcatc agcgcttcga
     1921 gcagcgcgcg cgcaccagct tggacgtgcc cgtgcaggtt cgtcgctcct cgccagaacg
     1981 aaacgccgag ccagagcagc agcacggcga gcaagaacgc gccgtacgcg cccccgagca
     2041 ccggctgtgt gaccacgagg atggggacgc ccgtcaagag cacgatcgca atttgcaggg
     2101 tcgcgacgaa cgcacgccgc ggcgcggcgc tgagatcgac gcttccctcc tctggctccg
     2161 gcaaggcagc gctcgcgatg ctgattgcga gccggcgcgc cactccgacg atcgcggcgc
     2221 tcagaggaag ggtcaagatc aacgcgcccc cgagccgcaa gccctgaacg aggcgagtcc
     2281 ccatcccgaa ccggtgctcc acgtaggagc tcaggcgcgg gagggcgaga gaaaacccaa
     2341 tcgcgagtcc gactagcagc gcggcgtcga gcgcgagcca caggatgagc cggcgagtat
     2401 cggcggcgcg ggtgttgctg cgcggaccac tgcgcagctg ctcgagccac gttgcataca
     2461 acgtgacgaa ggtctggatc ggccgtggca tcttgcgatc cacgaaattc gcgaccgggt
     2521 cggacgctcg aatcagccac ggcgtggtca gggtagtaat ggccgagacc gcgaccgcga
     2581 cggggtacag gaactcgccc gtcgccttca acgacaggcc gagactcgcg atgatgaacg
     2641 aaaactcccc gatttgagcg aggctcatcc ccgaccggag cgaagtgcga acgctgtttc
     2701 cggtgagaaa cgcgcccacg cccacgccca cgattttccc cacgatcacg acgaccgtga
     2761 gcaccaagac cgcgagccag tgctgcgcga tcaacgcggg atcgatcagc acgcccaccg
     2821 agacgaagaa gatcgcggcg aacatgtcgc gcaccggctg caccaggtgc tcgatcgtct
     2881 cgtgctcacc tgactcggcg atgagcgaac ccgcgatgaa tgcgccgaga gcgaccgagt
     2941 agccgagctc gtgcgcgagc agcgaaacgg cgaagcaaaa gccgaccgcg gcgacgagcg
     3001 tggtctccgg gcgctgcagt cgcagcaccg cgcggacccc ccgaggcact acgagcaacc
     3061 cgaccacgac gagccccgcg aggaagagcg ccagctttcc cgaagtggcc gccatcgta
//
LOCUS       DASTKS010000231         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_36882,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000231 DASTKS010000000
VERSION     DASTKS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36882"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VFQ35_04850"
     CDS             complement(<1..1532)
                     /locus_tag="VFQ35_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9929990.1"
                     /translation="MIRLALKNPIAVLMLSVSLIVFALVVTPRLSVDTFPELTPPVLV
                     IGTQVPGMGPKDVEKTISWRLEKYVSATPGVDHVQSVSRSGLSVIYVWLKWGTDLNAA
                     QSLVQQQVAFAMSSIPKSLGVVPPFVLQYDPTNAPVVQIAVYGGGLSGPQLYDYAANV
                     IEPLIEGIPGVASASPNGGRERQINVVVDPSRASSRGVTSSDVSNAVARANALLPSGH
                     FIANAFDANVYTNAVPKRIEEIGNSVVKLVDGKPILIRDVARLEDGGSPDTQAVGIDG
                     REAVYLNVLRVPGGNVIQIVDSIEAALKTLRDLPPGLKVTPVFEQSTFVKNTYDGLKR
                     EMLQAFCLVAAVILIFLQSPRSVLIAGIAVPISFAAILLVLYVTGQTLNAFTLGGLTL
                     ALGPLVDISVVVLESIHREKAKGKGPVQAALDGAGAVALPALAATLSTVAVLLPVVLL
                     SGLAKKLFAPLALTVASGMLAGYLVSMSVTPVACRYLLKHTHGGRLARAFERAIDGFA
                     ES"
     gene            complement(1529..>2688)
                     /locus_tag="VFQ35_04855"
     CDS             complement(1529..>2688)
                     /locus_tag="VFQ35_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9929991.1"
                     /translation="PKATPPAARRDFRVPLVLALSTLGIVAAGLTLLRRASASTNGVA
                     LAASAKGVSAVAARGARFRERRNYVGTLQPWLAAKVGPQLVSAYVSTVLVRPGMTVRA
                     GDVVATLDCRNSSAANDAVLSQARAAEARQRALASETARFQNLLDGGFVSPNEVEQRV
                     AQTAAETAQLEALRAQSVGKRLEVNDCILRAPFDGEVATRAADPGSYVRPGTAIVSIV
                     DRHLLRLVGDVPETDFEAVAPKTPVHFRLLSNGLELQGAVSRRAPAADDTTRTVRFEV
                     DIEPQKRDLPVGTSAQIELEVGEAADAVEIPLTSARVRGKSATLFVVDGNTARKREVK
                     VLGERGGSLFVTGEVAPGNLVVTEGRGLLANGDAVIVKDVSKEKPGAEAAP"
BASE COUNT          457 a          936 c          868 g          427 t
ORIGIN      
        1 tagctctcgg cgaagccgtc gatcgcccgc tcgaaagcgc gcgcgaggcg cccgccgtgc
       61 gtgtgcttga gcaagtagcg acacgcgacg ggcgtgacgc tcatgctcac gaggtagccc
      121 gcgagcatcc ccgaggcgac cgtcaaggcg agcggagcga acagcttctt ggcgaggcca
      181 gacagcaaga ccaccggcaa caacaccgcg accgtcgaga gcgtcgccgc gagcgcgggc
      241 aacgccaccg ctcccgcgcc gtcgagcgcc gcttgcacgg gacccttccc cttcgccttc
      301 tcgcgatgta tcgactcgag cactacgacc gagatgtcga ccagcggccc gagcgcgagg
      361 gtcagcccgc cgagggtgaa ggcgttcagc gtctgccccg tgacgtacag aaccagcaag
      421 atcgccgcga acgaaatggg caccgcgatg ccggcgatca acaccgagcg ggggctttgc
      481 aagaagatca ggatgacggc ggcgacgagg cagaaagcct gcagcatctc gcgcttcaac
      541 ccgtcgtagg tgttcttcac gaaagtcgac tgctcgaaga cgggcgtcac cttcagcccc
      601 ggtggcaggt cgcgcagcgt cttcaaggcg gcctcgatcg agtcgacgat ttgaatcacg
      661 ttgccgcccg gaacgcggag cacgttcagg taaacggctt cgcgaccgtc gatgccgacc
      721 gcctgcgtat ccggcgaacc accgtcctcg agccgcgcga cgtcgcgaat gaggatcggt
      781 ttgccgtcca cgagcttcac caccgaattg ccgatttcct cgatgcgctt cgggaccgcg
      841 ttcgtataaa cgttggcgtc gaacgcgttc gcgatgaagt ggcccgaggg caggagcgcg
      901 ttcgcgcgcg ccacggcgtt gctcacgtcg gacgaggtca cgccccgcga gctcgctcga
      961 gatgggtcga ccacgacgtt gatctggcgc tcacgtccgc cgttcggcga ggcgctcgcg
     1021 acgccgggga tcccctcgat gagcggctcg atgacgttgg ccgcgtagtc gtagagctgc
     1081 ggcccggaga gcccgccgcc gtacaccgcg atctgcacta cgggggcgtt ggtggggtcg
     1141 tactgcagca cgaagggtgg cacgacgccg aggctcttcg ggatggacga catcgcgaac
     1201 gcgacctgct gctgcaccaa cgactgcgcc gcgttgaggt cggtccccca cttcaaccaa
     1261 acgtagatga cgctgaggcc gctgcgcgaa acgctctgaa catggtcgac gcccggagtc
     1321 gcgctgacgt acttttcgag tcgccagctg atcgtctttt cgacgtcctt cgggcccatc
     1381 ccgggcactt gggtgccgat caccagcacc ggcggcgtca gctccgggaa cgtgtcgacg
     1441 ctgagcctcg gggttacgac gagcgcgaag acgatgagcg agacgctcag catcaagact
     1501 gcgatcgggt tcttgagcgc gagccgaatc acggcgctgc ctccgccccc ggcttctctt
     1561 tcgacacgtc cttgacgatc accgcgtcgc cgttcgcgag cagcccgcgg ccttcggtca
     1621 cgaccaggtt gcccggcgcc acctcgcccg tcacgaacag cgaaccgccg cgctcgccga
     1681 gcaccttcac ctcgcgcttg cgcgccgtgt tgccgtcgac gacgaacaac gtcgcgctct
     1741 tgccgcggac gcgcgcgctc gtgagcggga tttcgaccgc gtcggcggcc tcgcccactt
     1801 cgagctcgat ttgagcgcta gttcccacgg gcaagtcgcg cttttgaggc tcgatgtcga
     1861 cttcgaagcg tacggtgcgc gtcgtgtcgt ccgcggccgg cgctcggcgg ctcaccgcac
     1921 cctggagctc caacccgttc gagagcaacc ggaaatgcac cggcgtcttc ggggctacgg
     1981 cttcgaaatc ggtttcgggg acgtctccga ccagccgcaa caagtgccga tcgacgatgc
     2041 tcacgatggc agtgcccgga cgcacgtaag agcccggatc ggcggcgcgc gtcgccacct
     2101 caccgtcgaa gggcgcccgc aaaatgcagt cgttcacctc caagcgcttg cccaccgact
     2161 gagcgcgcag cgcctcgagc tgcgccgtct cggccgccgt ctgcgccacg cgctgctcga
     2221 cctcgttcgg tgacacgaac ccaccgtcga ggagattttg aaagcgcgcc gtttcgctag
     2281 cgagcgcgcg ctgacgtgct tccgcggcgc gcgcctgcga caacactgcg tcgttcgcgg
     2341 cgctgctatt gcgacagtcg agcgtcgcga cgacgtctcc cgctctgacc gtcattcccg
     2401 gccgcaccaa caccgtgctc acgtaagccg agacgagttg aggcccgact ttcgccgcga
     2461 gccaaggctg cagcgttccg acgtagtttc tgcgctcgcg gaagcgagca ccgcgcgccg
     2521 ctaccgcgct cacacctttg gccgacgccg cgagcgccac gccgttggtg ctcgccgaag
     2581 cccgccgcaa caacgtcaga ccggcggcga cgatcccgag cgtgctcagg gccaaaacga
     2641 gagggactct gaaatcgcgc cgagcggctg gtggggtcgc tttgggcg
//
LOCUS       DASTKS010000232         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_37225,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000232 DASTKS010000000
VERSION     DASTKS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37225"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..431)
                     /locus_tag="VFQ35_04860"
     CDS             complement(27..431)
                     /locus_tag="VFQ35_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929992.1"
                     /translation="MDKELVAVAQKCLSSAYDGSLTFPSIVGRLVTAGFESYGVDYRS
                     AKATYFLPNGESVELALPKSDGGVAAEFNPAVVERAVREAQANAPGYTYAGFCAKVKA
                     AGCAGYMVSFLGRRVVYFGRTGETHVEHFPKN"
     gene            complement(892..1674)
                     /locus_tag="VFQ35_04865"
     CDS             complement(892..1674)
                     /locus_tag="VFQ35_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9929993.1"
                     /translation="MAESVELAVSVPGLRASRCTTETLNTGLKSYYAVGRIERGRPEY
                     WSRGKVWRGAPGSIQVKEPGDVHRDLNHDGPTTFTVVVLPPEEVARVRTKGKVFAMPH
                     IDADDERAAPFQRLHDAVCAGADRLSLEVAVAEAISSFALFGNATPERTRPVRRAMEY
                     LREQLTEPISLDDLARHAALDKFHLCRAFRAQVGMPPHAYLTHLRVQRAKELLAQGMR
                     ASELAPHVGLYDQSQLTRHFRRIVGMTPARYATAQVGQSPAP"
     gene            1824..2699
                     /locus_tag="VFQ35_04870"
     CDS             1824..2699
                     /locus_tag="VFQ35_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9929994.1"
                     /translation="MNNPLVALVTGANKGIGLQIAKDLVARGVTVLLGSRDLERGEAA
                     AKSISTNLPSAAQPERSASAPGSPTASSAQPERSASAPGSPPPQRIGSVRAIQLDVTN
                     ADSIAAAAERIRREFGRLDILINNAGISQSGTWNHESFEQKVAAGRPSVAPLSEVRAI
                     FETNVFGLIAVTQAMLPLLREAPAGRIVNVSSEVGSLTLQTDPKNPYRIPSASYVPSK
                     TAVNAITVAFANELEKTRIKVNAACPGFTATDLNNFRGTRSVEQAAREPVRLALLDDD
                     GPTGTFSNEFGPLPW"
     gene            complement(2733..>3477)
                     /locus_tag="VFQ35_04875"
     CDS             complement(2733..>3477)
                     /locus_tag="VFQ35_04875"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9929995.1"
                     /translation="FSQRFKRDGCKEGGAGIGTWLSLEAPDELAREPAQVGLWTTRGV
                     RLFAIGGTTDNGLASSATPTGPGAITGLTAAGRDVVKRILEAGALVDVSNASELSIDE
                     VTQMAEKAGVPVVAGHSNARALADAAWNLSDPQIRGIAKTGGVVGVTARHGMLATGRN
                     ATMRHLVEQIRHMVRVAGPDHVAIGSSFEDGVGPVADFKSAADYPRLARALSDAGLSQ
                     DVIERVFYKNALRVLCPTEAPRAKRALER"
BASE COUNT          657 a         1228 c         1012 g          580 t
ORIGIN      
        1 ccgcgactga cgacgttctg cgcgattcag ttcttcggga aatgctcgac atgcgtctca
       61 ccagtccgcc cgaagtagac aacgcgcctc ccgagaaaag agaccatgta cccggcacaa
      121 cccgcggcct tcacttttgc gcaaaagcct gcgtatgtgt agccgggagc atttgcctgc
      181 gcctcgcgca ccgctcgctc gactacggct ggattgaatt cagccgcgac gcccccatcg
      241 ctctttggaa gcgcgagctc gacgctttca ccattcggga gaaagtacgt cgccttggcg
      301 cttcgatagt cgaccccgta actctcgaag cctgccgtga ctagcctccc gacgatggaa
      361 gggaaagtca acgagccgtc gtacgcagac gacaagcact tctgagctac cgccactaat
      421 tccttgtcca ttgttgctcc tgttctacga tgcgaaacca gaacgcccaa gccgaaggga
      481 gggcgctggc tgaagagaaa ggcgctggct gaagagaagg cgctggcctg aagagaaggc
      541 actggcctga agagaaggcg ctggctgaag agaaggcgct ggctgaagag aaggcgctgg
      601 cctgaaggtc gcctaaaaac gaccatccca acgcgcgcaa agagccgccg cgaccagctc
      661 acagtcaaca gcctaccgca ggatagtttc ctaggcaacc attctctgct cacgcgccgc
      721 gcggtcgccc gagtcgcgcc tttgcgacgg ctgacgtctt gcctggctgc gcgcggagtt
      781 ctccgaagcg cggagttctc cgaagcacgg cggttccgac gcggcgaacg gagcaattcg
      841 gcgggagctc gtgcggcgac gcgcctgaaa gtgcacgaac ctcggccgga gctagggggc
      901 ggggctttgc ccgacctgcg cggtggcgta gcgcgcgggc gtcatcccga cgattcggcg
      961 gaagtgacgt gtcagctggc tttggtcgta aagcccgacg tggggagcaa gctcggaggc
     1021 tcgcatgcct tgcgccaaga gctctttcgc ccgctgcacc ctcaagtgcg tgaggtaagc
     1081 gtgcggcggc atcccaacct gcgcgcgaaa ggcacggcac aggtggaact tgtcgagcgc
     1141 cgcgtggcgc gcgaggtcat ccagcgagat gggctcggtc aactgctcgc gcagatactc
     1201 catcgcgcgc cgtactggcc gcgtgcgttc cggggttgcg tttccgaaca aagcaaagct
     1261 cgagatcgcc tctgctactg ccacctcgag cgacaaccga tccgcaccgg cgcagaccgc
     1321 gtcgtgcagt cgttggaacg gtgccgcgcg ctcgtcatcg gcgtcgatgt gcggcatcgc
     1381 gaacaccttg cccttcgttc gcacacgcgc cacctcctcc ggcggcagca ctacgactgt
     1441 gaaggtcgtc ggaccgtcgt ggttcagatc gcgatgcacg tcgccgggct ccttcacctg
     1501 aatcgaaccc ggcgcgcctc gccacacttt gccgcgactc caatactccg gccgcccccg
     1561 ctcgatgcgc ccgaccgcgt agtagctctt gagccctgta ttcagcgtct cggtcgtgca
     1621 acgcgacgcg cgcagcccag gcaccgaaac ggcaagctcg acagactcgg ccatcccccg
     1681 aggatagctc ccggcgtcgg ctgccgcttg gcgattcttg gcagaacttc gcatcccgca
     1741 cggcgtcgaa actgcgagaa tcacctgaca aatcgccaag aaacgccaag tgcatccgct
     1801 ccgccgcccc caccttttgg cgcatgaaca acccactcgt tgccctcgtc acgggggcaa
     1861 acaaaggcat cggcctccaa atcgccaagg acctcgtcgc ccgcggcgtc accgtccttc
     1921 tcggctcacg cgacctcgag cgcggcgaag cagccgcgaa gagcatctcg accaaccttc
     1981 cgagcgcggc tcaacctgag cgttcggcct ccgcgcccgg ctcacctacc gcttcgagcg
     2041 cacaacccga gcgttcagcc tccgcgccgg gttcacctcc gccgcagcgc atcggcagcg
     2101 ttcgtgcaat ccaactcgac gtcacgaacg ccgactcgat cgccgccgcg gcggagcgca
     2161 ttcgaagaga gttcggtcgc ctcgacatcc tcatcaacaa cgccggaatt tcccaaagcg
     2221 gcacgtggaa ccatgagtcc ttcgagcaga aggtcgcagc cggacgcccg agcgtcgctc
     2281 cactttcgga agtgcgagcc atcttcgaaa ccaacgtctt cggcctcata gccgtcaccc
     2341 aagccatgct tccgcttctg cgcgaagcgc cggcgggtcg tatcgtcaac gtgtcgagtg
     2401 aagtgggctc cctgaccctc caaaccgacc ccaagaaccc ttatcgcatc ccgagcgcga
     2461 gctacgtccc ctccaaaacg gccgtcaacg cgatcaccgt tgctttcgcg aacgaactcg
     2521 agaaaacgcg catcaaagtc aacgcggcct gccccggctt caccgcgacc gatttgaaca
     2581 actttcgcgg cactcgctcg gtggagcaag cggcgcgcga acccgtgcgc ctggccctgc
     2641 tcgacgacga cggacctacg ggcaccttct cgaacgagtt tgggccactc ccctggtgaa
     2701 ttgctcgcga ggctcaaaag gccggcgccg tttcaccgct cgagtgcacg cttcgcacgc
     2761 ggcgcttccg tcgggcagag cacgcggagc gcgttcttgt aaaagacgcg ctcgatgacg
     2821 tcttggctca acccggcgtc ggagagagcg cgcgcgagcc gcggataatc ggcggcgctc
     2881 ttgaagtcgg cgacgggacc gacgccgtcc tcgaagctcg agccaatcgc gacgtgatcg
     2941 gggcccgcaa cgcgcaccat gtgccggatc tgttccacga gatggcgcat ggtcgcgttg
     3001 cgcccggttg cgagcatgcc gtgtcgcgcc gtgactccaa cgacaccgcc ggtcttcgcg
     3061 atgccacgaa tttgtggatc gctcaggttc catgcagcgt cggcgagcgc gcgagcattc
     3121 gagtgaccag cgaccaccgg cacgccggcc ttctctgcca tttgagtcac ctcgtcgatg
     3181 ctcagctccg acgcattcga aacatcgacc aacgcgccag cctcaaggat ccgcttcacc
     3241 acatcgcgcc ctgccgccgt gagccccgtg atcgcgcccg gtcccgtcgg tgtcgccgaa
     3301 ctcgcgagcc cattgtcagt cgttcccccg atcgcaaaca accgcacccc gcgcgtcgtc
     3361 cagagcccaa cctgcgctgg ctctcgcgcc aattcgtcag gcgcctccag actcaaccac
     3421 gtcccaatcc ccgcccctcc ttccttgcac ccatcgcgct tgaaccgctg agaaaac
//
LOCUS       DASTKS010000233         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_706071, whole genome shotgun sequence.
ACCESSION   DASTKS010000233 DASTKS010000000
VERSION     DASTKS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706071"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..2468)
                     /locus_tag="VFQ35_04880"
     CDS             complement(135..2468)
                     /locus_tag="VFQ35_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9929996.1"
                     /translation="MARILKVFAQGAEQNELSSYGDVFATYDAFAIVAPKHGYARALM
                     RKFPAQDITSQYELSLRGRKVPLPSDARRRSRIAPRHRAPSRAKHHYLVQFAGPVKES
                     WLRQIKKLGAEPRDLYRDFVYVVRADERTAQRVLELPFVLWVGHLPHEDRLSPGLKGE
                     SEPKVPRTRLRTGVYTVQVFDKRLVASVSSAAKRLGFSVITKAARGRSFVIQSRDDLA
                     KRTRMLRTLATVHGVRFIRARPIRRTSNDVASRLMGTKRVGRDNQSGLSGRGEIIAIC
                     DTGLDTGDAATIHADFKGRIVAIKSYPVSDEFRGDAANVGADDGPADFDSGHGTHVAG
                     SVLGDGAQSGFQKRPIRGLAHRAKLVFQAVEQEMKWRPHVAPSDRERYLLAGIPPNIE
                     ELFAYAYRKGARVHSNSWGGGDPGDYDEQCEQLDRFIWKHQDFTVMVAAGNDGTDSDA
                     DGRINLRSVTPPATAKNCITVGASENSRPDLNVETYGGWWPRDYPANPLRDDPMADNP
                     NQVAAFSSRGPTRDGRVKPDVVAPGTFILSTRSTMIAENNVAWRKFRDSNYYFFMGGT
                     SMATPLAAGAAALVREYLRTRQGVKRPSAALVKATLIAGAKRLPSLEVSTGVADNHQG
                     YGRIDLDAVLTARTHFVDVSRGLKTGQRYSLRLSVRAGSPALRVALAYSDYPGPSLVN
                     DLNLILTGPSGRKFTTAGLSVHGEIVLDTKNNTELVLVESPQRGAWALDVVASNVPQG
                     PQRFALVILGATGRGGCSPRRVRPRRRAAIAVRARSR"
BASE COUNT          452 a          848 c          858 g          508 t
ORIGIN      
        1 cgagaagcca tgaaacgtcc tttctggccg agctatcaaa acatacaccg cgagagttcg
       61 acatagtcaa ttcttctcgc caagctccga gtcgggaatg actctctatc agaagactgg
      121 gtgattcgac ctgctcagcg agagcgcgcc cgaacggcaa tggcagcgcg cctgcgcgga
      181 cgtacgcgcc gcggagaaca ccctccgcga ccggttgcgc cgaggatcac cagcgcgaag
      241 cgttgcggtc cttgtgggac gttcgaagcg acgacgtcca acgcccaggc acctcgttgc
      301 ggtgattcga cgaggacgag ctccgtattg ttcttggtgt cgagcacgat ctcgccgtga
      361 accgacagac cagccgtggt gaacttccgt ccactcggac cggtgaggat caagttgaga
      421 tcgttcacca gagacggccc cgggtagtcg ctgtaggcga gcgctacgcg cagcgcgggg
      481 ctgcccgcgc gcacgctgag gcggagtgaa tagcgctgac ccgtcttgag cccgcgcgac
      541 acgtcgacga agtgagtgcg cgccgtgagg actgcgtcga gatcgatgcg accgtagccc
      601 tggtgattgt cggcgacccc agtggacacc tcgagactgg ggaggcgttt tgcgccggcg
      661 atcagcgtgg ccttgacgag ggctgcgctc ggccgcttca ccccctgacg agttcggagg
      721 tactccctga cgagcgccgc tgctccggcc gcgagcggag tcgccatgct cgtcccaccc
      781 atgaagaagt agtagttgct gtcgcggaat ttccgccagg ccacgttgtt ctctgcgatc
      841 atcgtggaac gcgtcgagag gatgaaggtt cccggcgcga ccacgtcggg cttcacgcgg
      901 ccgtcgcgcg ttggcccgcg gctgctgaac gccgcgactt gatttggatt gtcggccatc
      961 gggtcgtcgc gcagcgggtt cgcaggatag tcgcgtggcc accagccccc gtaggtctcg
     1021 acgttcaggt ctgggcgcga gttctccgaa gcacctaccg tgatgcaatt cttcgcggtc
     1081 gccggcggcg tgacgctccg caagttgata cggccgtcgg cgtcactatc cgtaccgtcg
     1141 tttccggccg ccaccattac ggtgaaatcc tggtgcttcc aaatgaaccg atcgagttgc
     1201 tcgcattgtt cgtcgtagtc tccggggtca ccgccgcccc acgagttgga gtggacgcga
     1261 gcgccctttc ggtaggcata ggcgaagagc tcctcgatgt ttggggggat ccccgcgagc
     1321 aggtagcgtt cgcggtcgga gggtgcgacg tgcggccgcc acttcatctc ctgctccacc
     1381 gcttgaaaca cgagcttggc ccggtgtgcc agcccgcgga tgggccgctt ttgaaagccg
     1441 ctctgcgcac catcgccgag caccgaaccg gccacgtgcg tcccgtgacc tgaatcgaaa
     1501 tccgcggggc cgtcgtcggc tccgacgttg gctgcgtcgc cccgaaattc gtcgctgact
     1561 ggatagctct tgatcgcgac gatgcgccct ttgaaatccg cgtgaatggt cgcagcgtcg
     1621 ccggtatcga gcccggtgtc gcagatggcg atgatttctc cgcgccccga gagcccgctc
     1681 tggttgtctc ggccgactcg cttcgtcccc atcagcctac ttgcgacgtc gttactggta
     1741 cggcggatgg gccgcgctcg aatgaagcgt acgccgtgca cggtcgcgag cgtccgcagc
     1801 atcctcgtgc gcttggcgag gtcgtcgcga gactggatca cgaaggagcg accacgggcc
     1861 gccttcgtga tcacgctgaa gccgagacgc ttcgccgcgc tcgagacgct ggcgaccaag
     1921 cgcttgtcga acacttgaac cgtgtagacg cccgtgcgga gccgggtgcg cggcactttc
     1981 ggctcgcttt ccccttttag ccctggcgag aggcgatctt cgtgcggcag gtgtccgacc
     2041 cagagcacga aaggcagctc caagacgcgc tgcgcagtgc gttcgtcggc gcggacgacg
     2101 tagacgaagt ctcgatacaa gtcgcgcggc tcggcgccga gcttctttat ctgacgtagc
     2161 cagctctcct tcaccgggcc tgcgaactga accaagtagt ggtgcttcgc ccggctggga
     2221 gcgcgatggc gtggcgcgat ccgagagcgc cggcgcgcat cgctcggcag cggcaccttg
     2281 cgaccgcgga gagagagttc gtactgtgaa gtgatgtctt gcgccgggaa cttgcgcatg
     2341 agcgcgcgcg cgtaaccatg cttcggcgcg acgatcgcga atgcgtcgta cgtcgcgaag
     2401 acgtcgccgt aactcgaaag ctcattctgc tccgctccct gggcaaacac cttcagtatg
     2461 cgcgccacct tgcgacctcc acgttactcc tcgaactcga gcataagcag gttctggcgt
     2521 cgttggagag cattccccct cgctcgcgaa gaaatgacta aagcggagga cgtgcctccg
     2581 gaagtagtaa gtgtgaccgt gaagcgatct ttctggagcc accgcgtggc tccttgcgtc
     2641 gcgctggcgt tcaccgctct tctaac
//
LOCUS       DASTKS010000234         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1822925, whole genome shotgun sequence.
ACCESSION   DASTKS010000234 DASTKS010000000
VERSION     DASTKS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822925"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFQ35_04885"
     CDS             <1..663
                     /locus_tag="VFQ35_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9929997.1"
                     /translation="VFKAASKNYRCWMLFVTYGACFGIELFVHNVAASYYVDRFKLDL
                     QSAGLAAGSFGLLALFARALGGIISDRLARTRGLDARTALLFALMVGEGAGLLWFAKM
                     SSAGMAVFAMIVFGLFTHMACGATYALVPFIDRKSLGGVAGIVGAGGNVGAVAAGFLL
                     KAVGDVPQCFEILGVVALLSSAGALAIRFSASHKARETEAYEQARAESAPRPMVVAEP
                     VA"
     gene            complement(749..2188)
                     /locus_tag="VFQ35_04890"
     CDS             complement(749..2188)
                     /locus_tag="VFQ35_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929998.1"
                     /translation="MASPLAFWLMIMLIHAMSGLAPEGDVRARRAVRVVVVKAIFVTS
                     GSLVQARAGWGSPGFVLGLIIYLFPGFFLERVILPLRWPRVAYWFSRATWPLSFSDSD
                     QVGSCYYAARALGSGENPRLVEKFEKALAKGERLHGAGVVALGFLAVARREPEQALQF
                     FTVAQHLHERFISRSVRAVARDYLVKEAMRTADYRTALELGIDQQLRWSYFVARACQR
                     LLDDPAAPSNLALWFWWLLAPRRLRSYSLLQKAIQGGARENPPAPRTAREGLPGALGA
                     LASLLRRPKYVDTDGLKSVVAEVDRELGSTRTLASLEQRILDLGATAGADAVLRRLRD
                     ELVDCLVPLIEQSTRLRGTDDLEGTLAEAFLRAKGAALRELSTLRADYGRRADSEDTF
                     DPATEWALWAKFRALGERVLRVDPSAEAALFAEAFNPVLNFAVFQHNSRKQRALAHSM
                     YDWLHQLADSKAPDSDLLSKNMRAALSRP"
     gene            complement(2173..2529)
                     /locus_tag="VFQ35_04895"
     CDS             complement(2173..2529)
                     /locus_tag="VFQ35_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9929999.1"
                     /translation="MCLKHQDLLTRWRHNPFFVLEVATGSTRVEIERAAQKLLALMAV
                     GSPKANRCETPLGPIERDADQVRAALAALRDPNTRVVHEFWANVSLEEATGTSTEQGW
                     EEGERVAKVREPWPRR"
     gene            2682..>2907
                     /locus_tag="VFQ35_04900"
     CDS             2682..>2907
                     /locus_tag="VFQ35_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930000.1"
                     /translation="MRSLALLLGLAAVSCVSKPNNLFLGQPVSTPMSVHLHVSADAGE
                     TDELGGTAALIEAVTHELDERGIQNRLYTSD"
BASE COUNT          491 a          948 c          948 g          520 t
ORIGIN      
        1 gtcttcaagg ctgcgagcaa gaactaccgc tgctggatgc tgttcgtgac ttatggcgcg
       61 tgcttcggca tcgagctctt cgtccacaat gtcgcggcga gctactacgt ggaccgcttc
      121 aagttggatc ttcagtccgc cggtctcgcg gcgggcagct ttgggttgtt ggcgttgttc
      181 gctcgcgctc tcggcggcat catctcggat cgactggctc gtactcgtgg gctcgacgcg
      241 cgcactgcac tgctgttcgc gctcatggtc ggcgagggtg ccggtctgct ttggttcgca
      301 aaaatgagca gcgcgggcat ggcggtgttc gcgatgatcg tcttcggcct gttcactcac
      361 atggcttgtg gcgcgaccta cgccctcgtt ccattcatcg atcgcaagtc gctcggcgga
      421 gtggctggta tcgtcggcgc gggaggaaac gtcggcgccg tggcggcagg cttcctgctg
      481 aaagccgtgg gcgatgtgcc gcaatgcttc gagattttgg gagtggtcgc gttgttgtcc
      541 tcggccggcg cgctcgcaat ccgcttctcg gcgagccaca aggcgcgcga gacggaggcc
      601 tatgagcaag cgcgcgcgga gagcgctcct cgccccatgg tcgtggccga gcccgtcgct
      661 tgagctgcgt gaccacgcgg tgctctgctc cgcgcccttc tcaccgtgcc gatctcccgc
      721 cagtctcggt ccaggtgatg acgcggggtc atggtcgaga gagtgccgcg cgcatgttct
      781 tcgagagcag gtccgagtcc ggggctttac tgtccgcgag ctggtgaagc cagtcataca
      841 tggagtgagc gagggctcgt tgcttccggc tgttgtgctg gaagacggcg aagttcaaga
      901 cgggattgaa ggcttcggca aacaacgccg cttccgccga gggatcgacg cgcagcactc
      961 gctcgccgag cgccctgaac ttcgcccaga gtgcccactc ggtggcgggg tcgaaggtgt
     1021 cctcgctgtc ggcacggcgc ccatagtcgg cgcgtaacgt agacagctcg cgaagcgccg
     1081 cgccctttgc tcgaaggaaa gcttccgcca gcgttccttc gaggtcgtct gtcccgcgca
     1141 ggcgagtcga ctgctcgatg agcggcacga gacagtcgac gagctcgtcg cgcaggcgcc
     1201 gcagcaccgc gtccgcgccc gctgtcgcgc cgagatcgag gatgcgttgc tccaggctcg
     1261 ccagcgtcct tgtgctgccg agctcgcgat cgacctcggc gacgacgctc ttcagaccgt
     1321 ccgtgtcgac gtactttggg cgtcgcagaa ggctggcgag agcaccaagc gctccgggca
     1381 gaccctcgcg cgccgttcgc ggcgctggcg ggttctctcg cgcgcccccc tggattgctt
     1441 tctgtagcag ggagtagctg cgcagtcgcc gcggcgccaa cagccaccaa aaccacaggg
     1501 cgaggttcga aggcgccgcg gggtcatcga gcaaacgctg gcaagcgcgc gctacgaaat
     1561 aggaccagcg aagctgctga tcgatgccga gctcgagtgc cgttcgatag tcggcggtgc
     1621 gcatcgcctc cttgaccaag tagtcgcgtg ccacggcgcg caccgagcgc gagatgaagc
     1681 gctcgtggag gtgctgagcc accgtgaaga attggagcgc ttgctcgggc tcgcgccgag
     1741 cgacggcgag gaaccccaaa gcgacgacgc cagcgccgtg caggcgctca ccctttgcta
     1801 gcgccttttc gaacttctcg accaaccgcg ggttctcccc ggatccgagc gcgcgagccg
     1861 cgtagtagca ggagcccacc tggtccgaat cggagaacga cagcggccac gtcgctcggc
     1921 tgaaccaata cgcgacgcga ggccagcgca gcggaagaat cacgcgctcg aggaagaatc
     1981 ctggaaacag gtagatgatc aaccccagca cgaagcccgg cgacccccag ccggcccgcg
     2041 cttggaccaa gctgccgctc gtcacgaaga tcgctttcac gacgaccacg cgcacggcgc
     2101 gacgcgcacg cacatcgcct tcgggcgcca ggcccgacat cgcgtgaatg agcatgatca
     2161 tgagccagaa ggctagcggc gaggccatgg ctctcgcact ttggcgactc gttcgccctc
     2221 ttcccatcct tgctcggtgc ttgtgccggt cgcctcttcg agcgagacgt tcgcccaaaa
     2281 ctcgtggacg acgcgagtgt tcggatcccg cagtgcggcc agcgcagcgc gaacctgatc
     2341 cgcgtcgcgc tcgatcgggc cgagcggagt ttcacagcgg ttggcctttg gactgccaac
     2401 tgccatcagc gcgagcagtt tttgggcagc gcgctcgatc tcgacgcgag tcgagccagt
     2461 cgctacttcc agcacgaaga aggggttgtg gcgccagcgg gtcagcaagt cttggtgctt
     2521 caaacacacc tcgagctcga gccaaaaccg tgcacgtggt cctcacgatc tgccaaacgg
     2581 ggcgggcgta gcaccccgcc gagagcctgg ccagaacgcg ccgtcagctc tcgaacgcag
     2641 gtttgctcgg agcccacgag cgctgtatcg tgcgggaaat catgcgttcg ctcgcactgc
     2701 ttctcgggct cgcagccgtg agctgcgttt ccaaacccaa caatctcttc ctcgggcagc
     2761 ccgtgagcac gcccatgtca gtgcatcttc acgtctcggc ggacgcggga gaaacggacg
     2821 agctcggcgg caccgcggca ctcatcgagg ccgtgactca cgaactcgac gagcgcggta
     2881 tccaaaaccg cctctacacc tcggatg
//
LOCUS       DASTKS010000235         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_930112, whole genome shotgun sequence.
ACCESSION   DASTKS010000235 DASTKS010000000
VERSION     DASTKS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930112"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFQ35_04905"
     CDS             complement(<1..379)
                     /locus_tag="VFQ35_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930001.1"
                     /translation="MSSNPSSSRTRIHTPSQFPPGSVIAQRYRIDGVLAEGGMGIIYK
                     GWHLTLDQPIAIKVVRPEYADNAEAVSRFLNEARAVAALRAVNVAQVLDVGRIKGGVL
                     YMVMEYLEGADLRDVLDGEGQPLA"
     gene            639..959
                     /locus_tag="VFQ35_04910"
     CDS             639..959
                     /locus_tag="VFQ35_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930002.1"
                     /translation="MNQLDRSEQAETFDIMKMGRQVVAKQRRKRAGQVALGGVLITAG
                     ALLRGWLGVIGIGAGTYVIVRAVRDGVNSAIASRPRLDRNDAVDEASSLSFPASDPPA
                     MTAS"
     gene            994..1707
                     /locus_tag="VFQ35_04915"
     CDS             994..1707
                     /locus_tag="VFQ35_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9930003.1"
                     /translation="MSGASHTVQQNAARNAISEALRELTVVSDEARFQIHLFSLEAKR
                     RWNEIESKLRHLEARMAQGTDETDATKELSQAIRELLTHRRSAESPPTPVRALMARQV
                     ATCRSDQTLSDAAGAMARAQCNALAVVDEGGRFVGVLSEHDVCMGAYSSGTALSETPI
                     AATMRGDVETLKPDDTVANAAELMRFQKLEYVPVVDEEGSLAGMLWLRDLARRVEAED
                     EPSFDPAWLAATVVDAYAR"
     gene            complement(1695..>2442)
                     /locus_tag="VFQ35_04920"
     CDS             complement(1695..>2442)
                     /locus_tag="VFQ35_04920"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET9930004.1"
                     /translation="SIPENRVKWARIEAAKRRLLFTFAELGLPVKSKTEDPEQGLAFD
                     FKADTQDSVVLTGHDDGIVTLKLDEADPVLREQARVSLRERYRSLLGHFRHEVGHYYF
                     DRLVANGPAIEEFRKLFGDERQDYSEALKQHYARTAEGWCADFVSHYAQAHPWEDWAE
                     SFAHYLHMIDTLETAEAFTTSSKETRLYSRKDGPLIERWLELSIVLNALNRSMGLEDA
                     YPFQIGDGARKKIEFVQRVVEDHASGAQRA"
BASE COUNT          425 a          753 c          829 g          435 t
ORIGIN      
        1 cggcgagagg ctggccttcg ccgtcgagca cgtcgcgtag atcggcgcct tccaggtact
       61 ccatcaccat gtagagaacg ccgcccttga tgcggcccac gtccaggacc tgagccacgt
      121 tgacggcgcg gagcgccgct accgcgcgcg cttcgttcaa gaaccgggac accgcttcgg
      181 cgttgtctgc gtattcgggt cgcaccacct tgatcgcgat gggttgatcc aaagtcaggt
      241 gccaaccctt gtagatgatc cccattccgc cctcggcgag gactccgtcg atccggtagc
      301 gctgcgcgat gaccgagcct ggcggaaatt gcgaaggggt gtggatgcgc gtgcgcgacg
      361 acgacggatt tgaggacatg atctctcgct ggctcggcag taaggcggtg caagcgccgg
      421 accaggcgat gatcgcctgc ttacgcggac ttccagatcg cccgacacgc gcaaaccaac
      481 cagagcgtca cacctgaggc gacgctgccg cgagggtcgc cgatgttctg gagtttctcg
      541 gggtcgatcg cgaagcggcg cgccgttccc caatggcctg cgctttgcaa tctcttcgaa
      601 aacgcgccct ttcggggcga gcacaaggga gtgttgcaat gaaccaactc gaccgctcgg
      661 aacaagcgga gaccttcgac atcatgaaga tgggccgaca agtcgtggcg aagcagcgcc
      721 gtaaacgcgc ggggcaagtc gccctgggcg gcgtgctcat caccgccggc gcgctgttgc
      781 gcggttggct cggcgtgatc ggaatcgggg cggggactta cgtcatcgtt cgtgcggtgc
      841 gcgacggtgt gaacagcgcg atcgcctcgc gacctcgctt ggaccgcaac gacgcggtgg
      901 acgaagcgtc ctccctgtct ttccctgcca gcgacccgcc tgccatgacc gcgtcgtgag
      961 gcgccgagcg gagcgacgac gaaggagagc ccgatgtccg gagcgagcca taccgttcaa
     1021 cagaatgccg cgcgtaacgc gattagtgag gccctgcgcg agctcacggt cgtgagcgac
     1081 gaggcgcgat ttcagattca cctgttctct ctcgaagcga agcgtcgctg gaatgaaatc
     1141 gagagcaagc tcaggcatct cgaagcgcgc atggcccaag gcaccgacga gacggacgcg
     1201 accaaggagc tctcgcaggc gattcgtgag ctcctgaccc atcggcgtag cgcggagagt
     1261 ccgccgaccc ccgtgcgagc gctgatggcg cggcaggtcg cgacgtgtcg ttcggatcag
     1321 accttgagtg acgcagccgg cgcgatggcg cgcgcccagt gcaacgcgct cgccgtcgtg
     1381 gacgaggggg gccggttcgt gggcgtcctc agtgaacacg acgtgtgtat gggtgcctat
     1441 tcgagcggca cggcgctgtc ggaaactccc atcgcggcga cgatgcgcgg tgacgtggag
     1501 accctcaaac cggacgacac cgtcgcgaac gcggcggagc tcatgcgctt ccaaaagctc
     1561 gagtacgtgc cggtcgtgga tgaagagggg agcctcgccg ggatgctctg gctgcgtgac
     1621 ctcgcacgtc gggtcgaagc ggaggatgaa cccagcttcg acccggcttg gctcgccgcg
     1681 accgtcgtcg acgcctacgc gcgctgagcg ccgctagcgt ggtcctcgac gactcgctgc
     1741 acgaactcga tcttcttgcg cgcgccgtcg ccgatctgga acgggtaggc gtcttcgagg
     1801 cccatgctgc gattgagggc gttgaggacg atgctgagct ccagccaacg ctcgatgaga
     1861 gggccgtctt ttcgcgaata gaggcgggtc tctttcgagc tcgtggtgaa agcttcggcc
     1921 gtctcgagtg tgtcgatcat gtggaggtag tgcgcgaagc tttcggccca gtcttcccag
     1981 gggtgggctt gggcgtaatg gctgacgaag tcggcgcacc agccctcggc cgtccgcgcg
     2041 tagtgctgct tgagggcctc ggagtagtct tgtcgctcgt cgccgaacag cttgcgaaac
     2101 tcttctatcg cggggccgtt cgcgacgagc cgatcgaagt agtagtgccc gacctcgtga
     2161 cggaagtgcc cgagcagact gcggtagcgt tcgcgtagcg acacgcgcgc ttgttcacgc
     2221 agcactgggt cggcctcgtc gagcttgagg gtcacgatac catcgtcgtg tccggtgagg
     2281 acgacggagt cttgagtgtc ggccttgaaa tcgaaagcca gaccttgctc ggggtcttcc
     2341 gtcttcgact tcaccggcaa gccgagctcg gcgaacgtga acaacaggcg gcgcttggcg
     2401 gcctcgatcc gcgcccactt cacccggttt tcggggatgg ag
//
LOCUS       DASTKS010000236         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1153351, whole genome shotgun sequence.
ACCESSION   DASTKS010000236 DASTKS010000000
VERSION     DASTKS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153351"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFQ35_04925"
     CDS             complement(<1..777)
                     /locus_tag="VFQ35_04925"
                     /inference="COORDINATES: protein motif:HMM:NF036911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HET9930005.1"
                     /translation="MDYSLATRLALLLVCATTWNVACTQQVDDIGEPPQGSAPEESAR
                     HHDLAPGLQPTHARDSLACDSIEQEEEGDFTPPEAQPYFLRHSIAELPVALLPAWQRA
                     DLAPAFERIRDERFQCDPAHPNFARRLTWLAPAVSCEARAELVSAKLAEWGYPRPGKV
                     FVFGSPDLVVSTPNSESGRVSWPWHVAAAVRVGDEAYVFDAALEPRRPLTVLEWASRM
                     APSVESLTAAVCDAAAFQPSSYCFGEPVSKTRTALAYERNH"
     gene            complement(899..1549)
                     /locus_tag="VFQ35_04930"
     CDS             complement(899..1549)
                     /locus_tag="VFQ35_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930006.1"
                     /translation="MTKFGTLVGFLSVPLLSCSSETAIVATLDVAGAAGASAGGKAGS
                     GAGGAAGAIQSMSGGAMQQSGGTAASSGGASNSSGGVGGGGAASGGAGGSSGGGVAGF
                     GGDTGGAVGDGMTLDAGAGGILVFPSSQTYCSCSETSVVCDSDGVSHLCLSNRCPQIT
                     IICAHECPCGAGDSTVGNPGVWYPEECFDPSTCSETYFCVSNSPEGPALIGDCSAL"
     gene            complement(1546..2694)
                     /locus_tag="VFQ35_04935"
     CDS             complement(1546..2694)
                     /locus_tag="VFQ35_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930007.1"
                     /translation="MARAELGGRAFAWCVAVAALGPTAARAAAPVNDVSRVDAPPRIE
                     VVLVGAAERDPALFERLRTLFPPPSLVILRKETGVVSSAVLTPERADTLYIWIRSSSR
                     NVARVYLAAREQSEESARYLYREVALESGLDEIGSESLAQIAHSAAEALWSREQEISK
                     LEVLRALAKESEAASSRALPASEAPSVTPALRPREAPPPARAPANEAVHVALGASFGT
                     HSGGDEGWLSEPGAFVMGSYGRASLRLGANYLMPAEFELLPARVRLSGGSAELRAGWL
                     AFAGRRARVRLEAGIGAWAGRWMAMIVADEPKAHANSASFLRGYGLAAGAFEWSLGSG
                     WIAARAELRTRFRKTDYEVEGVATSASSSYLNPGAALELGVVLDGGAR"
     gene            complement(2681..>3618)
                     /locus_tag="VFQ35_04940"
     CDS             complement(2681..>3618)
                     /locus_tag="VFQ35_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930008.1"
                     /translation="REMSRGDASDLLCRARREELSGEEHRRLEGLLESSAEARLMSAM
                     LSEFERESQVRAGDDVLLARIGARALRKSGGGFGEAPFGRRLTVRLRSRVALLVAAAV
                     LLVGSIAFGWRLTVSRRAASTPAPATSMQTPTSQSIVRAQTRKLDPPRVEEATALDVT
                     PLQEHAAPLTTTRKSAARPAPSAPVNGRSNEASELFANANLLRRQGNNDEAARMYQQL
                     ISEYSQSREAAPARLALGKLLRGRDPARALVQFRVLAEQRGALRPEALWGMAEASRSL
                     GQSAVESRALDDLVREFPDSPYADAARARIADGAR"
BASE COUNT          615 a         1307 c         1165 g          531 t
ORIGIN      
        1 atggttgcgc tcgtaagcga gcgccgtgcg ggtcttggac accggctcac caaagcagta
       61 gctcgaaggt tggaacgcgg ccgcgtcgca caccgccgcg gtcagcgatt cgacgctcgg
      121 ggccatgcgc gaagcccact cgagcacggt gagcgggcgt cgcggctcga gcgcggcatc
      181 gaagacgtac gcctcgtcgc cgaccctcac cgcagcggcg acgtgccacg gccagctgac
      241 gcggccgctt tcggagttcg gcgtggacac gactagatcg ggtgaaccga aaacgaacac
      301 cttgccgggt cgcggatagc cccactcggc gagcttggcg ctcacgagct ccgcgcgcgc
      361 ctcacagctc acggcgggcg cgagccaagt gagccggcgc gcgaaattcg gatgcgcggg
      421 atcgcactga aagcgctcgt ctcgaatccg ctcgaacgcg ggcgcgagat ccgcgcgctg
      481 ccacgcggga agcaacgcga ccgggagttc tgcgatggag tgccgcaaga agtacggctg
      541 agcctccggc ggcgtaaaat cgccttcttc ctcctgctcg atcgagtcac acgcgagcga
      601 atctctcgcg tgcgttggct gaagacctgg agcgaggtcg tggtgtcgcg ccgactcttc
      661 gggtgcgctt ccttgcgggg gctctccaat gtcgtcgact tgctgtgtac aagctacgtt
      721 ccaagtcgtc gcgcacacga gcagcagcgc gaggcgcgtc gccagcgaat agtccatcct
      781 cgaatactcc gtgagtcgcg cgtccttgcg cgctccaccc ctaccgtgtg ctcgaggcgc
      841 gaaacgggtg aagccaggag aacgaagtgc tcgacgtcgc ccgctctggg cggttcagtc
      901 acagcgccga gcagtcgccg atcaaggccg ggccttcggg cgaattcgac acgcagaagt
      961 aggtctcgct gcacgtgctc gggtcgaagc actcctcggg gtaccaaacg cctggattcc
     1021 caaccgtcga atcacccgct ccgcacgggc actcatgagc acagatgatg gtgatctgcg
     1081 gacaacgatt gctcaaacac aagtgcgaca ctccgtcgct gtcgcacacg acgctggtct
     1141 cggaacaaga gcagtacgtc tgcgagctcg gaaagacgag gatgccaccg gcgccagcat
     1201 ccaaagtcat tccgtcaccg acggcgccgc cggtgtcgcc gccaaaacca gccacgccgc
     1261 ccccggaaga tccgcccgcg ccgccggaag ccgcgccgcc tccccccact ccgccggacg
     1321 agttgctcgc gccgccgctc gacgccgccg taccgcccga ctgctgcata gcgccgccgc
     1381 tcatgctctg aatcgcgccc gcagcgccgc ccgcgccgga accggctttt ccgccggcgc
     1441 ttgcgccagc cgcacccgcc acgtccagcg ttgcgacgat tgcggtctcg ctcgagcagg
     1501 acaagagcgg cacggagagg aaacctacga gcgtcccgaa cttcgtcatc gcgcgcctcc
     1561 gtcgagcacg acgccgagct cgagcgccgc ccccgggttc aagtagctgc tcgaggcgga
     1621 cgtcgccacg ccttcgacct cgtagtccgt tttccggaac cgcgtccgaa gctccgcgcg
     1681 cgctgcgatc catcccgagc cgagcgacca ctcgaaagcg cctgcggcga gcccatagcc
     1741 ccgcaggaac gacgccgagt tcgcatgcgc cttcggctcg tcggcgacga tcatcgccat
     1801 ccaacgccct gcccaagcgc cgatcccggc ttctaggcga acgcgcgcac ggcgccccgc
     1861 gaacgccaac caccccgcac gaagctcagc cgagcctccg ctcaaacgaa ctcgcgctgg
     1921 caacagttcg aactcggccg gcatcaagta gttcgcgccg aggcgcagcg aggcacggcc
     1981 gtacgagccc atcacgaatg cgcctggttc actcaaccaa ccctcgtcac cgccgctgtg
     2041 agtgccaaag ctcgcgccga gcgcaacgtg gaccgcttcg ttcgcggggg cgcgcgcggg
     2101 cggcggcgcc tcgcgagggc gtaacgcggg agtaacgctc ggcgcttcgc tcgcgggtag
     2161 cgcgcgactc gaggccgctt cgctctcttt cgcgagcgcc cgcagcacct cgagcttgga
     2221 aatctcctgc tcgcgagacc acaaagcctc cgccgcggag tgggcaattt gcgcgaggct
     2281 ttcactgccg atttcgtcga gccccgactc gagtgcgacc tcgcggtaaa ggtagcgcgc
     2341 gctctcctcg gactgctcac gtgcggcgag gtaaacgcga gcgacgttgc gcgagctcga
     2401 acggatccag atgtagaggg tatcggcgcg ctcgggcgtg agcaccgcgc tcgagaccac
     2461 gccggtctcc ttacgaagaa tcacgagtga aggcggcggg aacaaagtgc gaagccgctc
     2521 gaagagcgct ggatcgcgct cggcggcgcc gaccagcacc acctcgatgc ggggaggcgc
     2581 gtccacgcgc gaaacgtcgt tcaccggtgc cgcggcccgc gctgcagtcg gaccgagcgc
     2641 ggccacggcg acgcaccagg cgaacgcacg accaccgagc tcagcgcgcg ccatcggcga
     2701 tccgagcccg ggccgcatcg gcgtaaggcg agtccgggaa ctctcggacg aggtcgtcga
     2761 gcgcccgtga ctcgaccgcg ctctgaccaa gactccggct cgcctcggcc attccccaga
     2821 gagcttccgg gcgaagcgcc ccgcgctgct cggcgaggac ccgaaactgc acgagggccc
     2881 gcgctgggtc cctacccctc aacaattttc cgagcgcgag ccgcgccggc gccgcctcgc
     2941 gcgattgcga gtattcgctg atgagttgct gatacatccg cgctgcctcg tcgttgttcc
     3001 cctgacgacg gagcaagttg gcgttggcga agagctcgct cgcctcgttg ctgcggccgt
     3061 tcaccggcgc actcggggcc ggacgcgcgg cgctcttgcg cgtcgtcgtc agcggggcgg
     3121 cgtgctcctg aagcggcgtc acgtccaagg ccgttgcttc ttcgacgcgt ggagggtcga
     3181 gcttccgagt ctgggcacgg acgatgctct gactggttgg cgtttgcatc gaagtcgcgg
     3241 gcgcgggtgt cgacgcggct cgcctcgaaa ccgtcaggcg ccaaccaaac gcgatgctgc
     3301 ccacgagcag cacggccgcc gccaccaaaa gagccacgcg cgagcgcaac cgcaccgtga
     3361 ggcgccgtcc gaacggcgcc tcgccgaagc ctcccccgct ctttcgcagc gcgcgcgcgc
     3421 cgatgcgcgc caaaaggacg tcgtcgcccg cacgcacttg gctctcgcgc tcgaattccg
     3481 agagcattgc gctcatcagc cgagcctctg cggacgattc gagcaagccc tcgaggcgcc
     3541 ggtgctcttc accgctcagc tcttcgcggc gagcccggca gagcagatcg ctcgcgtcgc
     3601 cgcggctcat ctcgcgtc
//
LOCUS       DASTKS010000237         7813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1377024, whole genome shotgun sequence.
ACCESSION   DASTKS010000237 DASTKS010000000
VERSION     DASTKS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377024"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..738)
                     /locus_tag="VFQ35_04945"
     CDS             complement(178..738)
                     /locus_tag="VFQ35_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930009.1"
                     /translation="MPLSVRLFWWRGIPIHGHITLVVGLGLASRLAFRPLAWVALLLI
                     VFIHELGHAFLLRRYGIPVLSITLHGFGGECESPDLHMSVWQRSVVAWGGVGAQAALF
                     CVTSALAELGLFAKSAVAAQFLVDLSAFNVATVVVNLLPFGGLDGRLAWRLPWFAFRR
                     AQHAWLRSRIVRIRSREERARREDLH"
     gene            complement(771..2165)
                     /locus_tag="VFQ35_04950"
     CDS             complement(771..2165)
                     /locus_tag="VFQ35_04950"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET9930010.1"
                     /translation="MNPRHLSELLVVLCLASCEPATVTVTPHGSAPPIRKVRLGLCED
                     YPEESRSLAEVRRDLGVLKAARVDVLRVSMGWDELEPKRDSYDFAFWDAMVDMAVGEF
                     KIQLIPYVAYTPEWNSDGTPTDFWKTPPRETREFGELMGILARRYRGRIHSWELWNEP
                     DNRDYWLGSAADYAQLLEAGATAVHASDPDARVVFGGLAGGVDFLREVFDVHHGGERL
                     DVVNVHSYYETWNPEPLETLPEYLERVAQIIEAHGGRQSLWMAEAGYSDFRGEGLDGA
                     RRAKSYEHTPDYQAVMLTKTLALALSHPKVELIAWYELKDARPSDAVIGDAHNRHLGV
                     DFADYTPKPAFSALGFMSQLFRPGFRSIDSELVLLSAPNADLTLRGFLTERAVVLIAW
                     LSTAAGAAAPLRVRLPYAARGAARRFDASGRARGTVPLREVGRGRELELEARPGEVEI
                     VRIGLGGRVALPAN"
     gene            2370..4202
                     /locus_tag="VFQ35_04955"
     CDS             2370..4202
                     /locus_tag="VFQ35_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9930011.1"
                     /translation="MSEDFAPRPLFRSIRKLAPLCREQWPELLRVALLGGASATLSAL
                     EPLSLKALFDRFVAKEGLIAALAPLGAFVAILTAREVCSLVQDRAFWRARLGISFGLL
                     QAMIERLHLLPLDYHRDHGVGATMTKIERGMTGVTGAFSEFAQQLFPAIVYLAVSLVV
                     MFRVDPRLSLVVLLFAPLPALIGVFASREQTQRESSLMQRWTRIFARFNEVLSGILVV
                     KSFVMEEREKRRFLGGVNEANQIVLRGVATDSKFNTIRNGVIAVARIIALAIGGTLVM
                     KGEIGVGTLVAFLAYLSGVFQPVQALTGIYQALRRATVSAESVLSILEAQVSLGDAPD
                     ARDAGSLRGDVEFKDVSFGYRRDKLLLQHVDFRARPGEVIALVGPSGAGKSTLMSLLQ
                     RLYAPTSGSVLLDGEDIGSFEQRSVRAQIGVVLQEGMLFSDSVRDNIAFGRPGATQGE
                     IEAAARAANAHEFICKLEHGYDTAVGERGCKLSGGERQRIAIARALLKDAPILILDEA
                     TSALDSENEEKVQEALARLTKGRTTFVVAHRLATVMSADRILVLRDGTIVESGNHAQL
                     MQAGGYYATLARKNFLPSSSGRSSRPPGPRSTPPRTLEGYVAQA"
     gene            complement(4219..7059)
                     /locus_tag="VFQ35_04960"
     CDS             complement(4219..7059)
                     /locus_tag="VFQ35_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9930012.1"
                     /translation="MSRRKVIAFLTNNAGMRSDYQGLLRQGIEQACIERDIDLWVYAG
                     RSDWRSWGAAQARVYEMVSPNRVDGVVVAASCIAAALSEETVLELVTRRSIVPTCAVG
                     LHCPTVASVVVDNASGSAQLARHFASAHGARRFVYVAGPAGHNESEARLRGTRSALAE
                     HGLELPATSVIHANFAFSAGEDAAKEFLRRGVHFDALIAANDDMAVGALGALKGAGLR
                     CPQDVLIGGFDDAASSRAASPPLTTVRQPIVELGASAVARVLRAWEGKPDVELLTLPT
                     EAVFRESCGCGGIDLWSEESAASGSEERIAESLTPLIGDRARREEWARALCRSLKGER
                     GELAGSFSRTVRALLERLGDEAPVHELQHVIVQLRDAVPEQEVTRELETAMRRTLVDL
                     GQAMHRREEKRRVHDDDLTERLRANWDRLSASAFDLPSLRSCLEIQLPSLSICNAFVG
                     VYARDDLDSLQPLVCVMNGEPVALKEQSYPASLLLPHGALMTSKRCSLAVVPLTFEWQ
                     NLGIAVLELPKSHELYAVLREQIGSAVRTMRLHEDILQQARLSAQAEEEKRVTAERLR
                     SLSLVAGGVAHDLNNVLGPLVGLPETISSELQRAIGDAAPPGIFEDIETLRLAGLRAA
                     DTIQDLLTLGQQNAAPKQVLDLNRLLASECRSFIALGDRSPRISICVVTGDEPLPVRA
                     SKAHLMRAISNLVLNAIDAIEGSGSVTVRAASRAFTEPHRGVETIDPGQYVVIEVEDT
                     GAGIAPENMSRILEPFFSSKKSNERRGGRGLGLAIVHRIAKDSLGFVGIKSEVGRGTT
                     FSLYLPLERDRPVRVSSRPAPPMRGTERILVIDDEPVQLRTARRVLENLGYTVVTAPS
                     GEIGLGLLSEGDEPTEFDLVMVDMMMPGLDGIATLERIRAVRPNQKALIVTGYTPLEA
                     DRGNDPWLRKPYTQQSLSRAVRGALDGVTARE"
     gene            7273..7551
                     /locus_tag="VFQ35_04965"
     CDS             7273..7551
                     /locus_tag="VFQ35_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9930013.1"
                     /translation="MATVEITKHLWEFLPVLRDKTLNVEASTVAEVIREVERIAPGFT
                     YYICDERGCLRTHVNIFVGQERICDRTRLSDPVNDDSRVLIMQALSGG"
     gene            7617..>7813
                     /locus_tag="VFQ35_04970"
     CDS             7617..>7813
                     /locus_tag="VFQ35_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930014.1"
                     /translation="MRVLVGTRKGTFFVEKQAGSWKPRLVGHRGVGVNFVARDPHRGT
                     LWAALGHGHWGAKLSRSDDDGA"
BASE COUNT         1321 a         2531 c         2613 g         1348 t
ORIGIN      
        1 ctagggggtg tcccaagttc ctgcccgtcg cgagagccaa gaaacgcgag cgagaagcgc
       61 gcccgaggag tgagcactgg aggaaggctg aagcctttcg aggagcggag cgacgaggcc
      121 gtgcttcgca gccgcaggat cttggctcgc agcagggcgg ggaacttggg acacccccta
      181 atgcaggtct tcgcggcgag cgcgctcttc tctcgagcga atgcgaacga tgcgggagcg
      241 aagccaagcg tgttgggctc ggcggaaggc gaaccagggc aagcgccacg cgagccttcc
      301 gtcgaggcca ccgaaaggga gcagattgac gacgaccgtt gcgacgttga acgcgctcaa
      361 gtcgacgagg aactgcgcgg cgacagcgga cttcgcgaac agcccgagtt cagccagcgc
      421 agaggttacg cagaagagcg cggcctgggc accgactccg ccccaggcga cgacggagcg
      481 ttgccagacg ctcatgtgca agtcggggct ttcgcactct ccaccgaagc cgtgcagcgt
      541 gatgctgagc acgggaatgc cataacggcg cagcagaaac gcgtgcccca actcatggat
      601 gaacacgatg agaaggagcg cgacccatgc cagaggacga aacgcaaggc ggctcgcgag
      661 gccgagcccg accaccagcg tgatgtgccc gtgaatcgga atcccacgcc accagaacaa
      721 cctgacgctg agcggcatcg cgagcagaat atctgctctt gcgaggcagc tcaattcgca
      781 ggcagcgcga cgcgaccgcc gagcccaatt cgcacgatct cgacttcacc aggtcgcgct
      841 tcgagctcga gttcgcggcc gcgccctacc tctcgcaacg ggacggtgcc gcgcgctcga
      901 ccgctcgcgt cgaagcgtcg tgcggccccg cgcgccgcgt acggcaggcg aacgcgtagc
      961 ggcgcggccg cgcccgccgc cgtgctcagc cacgcgatca agacgacggc gcgctcggtg
     1021 agaaatcccc gcagcgtgag gtccgcgttc ggcgcggaca gaagcacgag ctccgaatcg
     1081 atcgagcgaa agccagggcg aaacagttgg ctcatgaacc cgagtgcgga aaaggcgggc
     1141 ttcggcgtgt agtcggcgaa gtcgacgccg aggtgccggt tgtgagcgtc accgatgacc
     1201 gcgtcgctcg ggcgcgcgtc tttgagctcg taccaggcga tgagctccac tttggggtgg
     1261 gagagggcga gcgcgagagt cttggtcaac atcaccgctt ggtagtcagg cgtgtgttcg
     1321 tagctcttcg cgcgccgagc gccgtcgagc ccttcgccac gaaagtcgct gtagcctgcc
     1381 tccgccatcc agagcgattg cctccccccg tgagcttcga ttatctgcgc gactcgctcc
     1441 aagtactcgg gcaatgtctc gagtggctcc gggttccaag tctcgtagta gctgtgcacg
     1501 ttcacgacgt ccaggcgctc gccgccatga tggacatcga agacctctcg taggaagtcg
     1561 acgccgcccg cgagcccccc aaagacgact cgagcgtcgg gatcggaggc gtggacggcc
     1621 gtcgcccccg cttcgagcaa ctgcgcgtag tccgccgcgc tgccgagcca gtagtcacga
     1681 ttgtccggct cgttccagag ctcccaagag tggatgcggc cgcgataacg tcgcgcgaga
     1741 atccccatca gctccccgaa ctcgcgtgtc tcgcgtggcg gagtcttcca gaagtccgtc
     1801 ggtgtaccgt cggagttcca ctccggcgta taagcgacat aggggatcag ctggattttg
     1861 aattcgccga cggccatgtc gaccatcgcg tcccaaaaag caaagtcgta cgagtcgcgt
     1921 ttgggctcga gctcgtccca acccatcgac acgcgcaaca cgtcgacccg agctgccttc
     1981 aagacgccca aatctcgccg cacctccgcg agtgatcggc tctcctccgg gtagtcttca
     2041 cacaagccga gccgcacttt gcgtatcgga ggagcgctgc cgtgaggagt gaccgtgacc
     2101 gtcgcgggtt cgcagctggc gagacaaagc acgaccagga gctcggacaa gtgtcgcggg
     2161 ttcacggtgg agctcagact acacccgagc tcgcttcgtt ggatggaacg agacaaaacg
     2221 gatctgctgc gcgcgctgac atgggattgc gccttgttac gctagggcac aatcctgact
     2281 ttcggcagtt gtgctcactc tttgcctcgc cgattgcaca ttggggctat cggcgcgtcc
     2341 gcttccgccg ttgtcgcttt gcgcagacga tgtccgagga tttcgcgccg cgcccgcttt
     2401 tccgttcgat tcgcaagctc gccccgctct gtcgagagca gtggccggaa ttgctgcgcg
     2461 tcgcgctgct cggcggcgcc tcagcgaccc tttctgcgct cgagccattg agcttgaagg
     2521 cgctgttcga tcgcttcgtc gcgaaggagg gcctcatcgc ggcgctcgcg ccgttgggcg
     2581 cgttcgtcgc gattttgacg gcgcgcgagg tgtgctcgct cgttcaagat cgtgcctttt
     2641 ggcgggcgcg tctcggcatt tcgttcgggc tgctgcaagc gatgatcgag cgattgcact
     2701 tgttgccgct cgactaccac cgcgaccacg gagtcggcgc gacgatgacc aagatcgagc
     2761 gcgggatgac gggcgttacg ggcgcgttca gtgagttcgc ccagcagctg ttccctgcga
     2821 tcgtgtacct cgcggtgtcg ctcgtggtga tgttccgggt ggacccgcgc ctgtcgctcg
     2881 tggtgctgct cttcgcgccc ttgccagcgc tgatcggtgt gttcgcgtcg cgtgaacaaa
     2941 cccaacgaga gagcagcttg atgcagcgct ggactcgaat cttcgctcgc ttcaacgagg
     3001 tgctgagcgg tatcttggtg gtgaagagct tcgtcatgga agagcgcgag aagcgccgct
     3061 ttctcggtgg tgtcaacgag gcgaaccaga tcgtgctgcg aggcgtcgcg accgactcca
     3121 agttcaacac gattcggaac ggcgtcatcg cggtcgcgcg catcatcgcg ttggccatcg
     3181 gcggtacgtt ggtgatgaag ggcgagattg gggtcgggac gctcgtcgcc ttcctcgctt
     3241 acttgagcgg tgtgttccag ccggtgcagg cgctcacggg catctatcag gctctgcgcc
     3301 gcgcgacggt gtcggccgag tcggtgcttt cgatcctcga agcgcaggtt tctctgggcg
     3361 acgctcccga cgcgcgcgac gcgggttctt tgcgcggcga cgtcgagttc aaggacgtga
     3421 gcttcggcta ccgccgagac aagctgctgc tgcagcacgt cgacttccgg gcgcggcccg
     3481 gcgaagtcat cgcgttggtc gggccgagcg gagccggaaa gtcgaccttg atgtcgctct
     3541 tgcagcgcct ctacgctccc acctccggct ccgtgctgct cgatggcgaa gacatcggta
     3601 gcttcgagca gcgctcggtg cgggctcaaa tcggagtcgt cttacaagag ggcatgctct
     3661 tcagcgatag cgtgcgcgac aacatcgcgt tcggccgccc gggcgcgacg caaggggaga
     3721 tcgaagcggc ggcgcgcgcc gcgaacgcgc acgagttcat ctgcaagctc gagcacggct
     3781 acgatacggc cgtcggtgag cgcggttgca agctctcggg cggtgaacgc cagcgcatcg
     3841 ccatcgcgcg cgcgctgctg aaagatgcgc cgatcctcat cctcgacgag gcgacttcgg
     3901 ctctcgactc cgagaacgaa gagaaggtgc aggaggcgct cgcgcggctc accaagggcc
     3961 gcacgacgtt cgtcgtggcc caccgcctcg cgaccgtgat gtcggccgat cgcatcttgg
     4021 tcttgcgcga tggcaccatc gtcgagagcg gcaaccacgc ccagctgatg caagcggggg
     4081 gatactacgc aacgctcgcg cggaagaact ttttgccgtc ttcgtccggg cgttcgtcgc
     4141 gcccgccagg gcctcggtcg accccgccgc gcaccttgga agggtacgtc gcgcaggcgt
     4201 gaacgagccg gagtggcgct actctctggc ggttacgccg tctagcgcgc cgcgcaccgc
     4261 tcgactcaag gactgctggg tgtacggttt tcgcagccac gggtcgttgc cgcgatccgc
     4321 ctcgagcggc gtgtagccgg tcacgatgag cgccttttga ttgggccgca cggcgcggat
     4381 gcgctcgagg gtggcgatcc cgtcgagccc cggcatcatc atgtcgacca tcacgaggtc
     4441 gaactccgtc ggttcatcac cttcactgag aaggccgagg ccgatctcgc cggacggcgc
     4501 ggtgacgacg gtgtatccga ggttctcgag gacgcgccgc gcggtgcgga gctgcaccgg
     4561 ctcgtcgtcg atcaccaaaa tgcgctcggt gccccgcatg ggaggcgcgg gccgggagct
     4621 gacacggacc ggccgatcgc gctcgagcgg cagatagagc gagaacgtcg tgcctcggcc
     4681 gacctcgctc ttgattccga cgaagccgag cgaatctttc gcgatgcggt gcacgatcgc
     4741 gaggccaagc ccgcggccgc ctcggcgctc gttgctcttc ttggagctga agaacggctc
     4801 gaggatccga ctcatgttct ccggagcgat gccggccccg gtgtcttcga cctcgatgac
     4861 cacgtactga ccggggtcga tggtttcgac cccgcggtgc ggctcggtga acgctcgcga
     4921 cgccgcgcgc acggtcacgc tgccggagcc ttcgatggcg tcgatcgcgt tgagcacgag
     4981 gttcgaaatg gcgcgcatca ggtgcgcttt cgaggctcgc accggcaggg gttcgtctcc
     5041 ggtgacgacg caaatcgaga tccgagggct ccggtctccg agcgcgatga agctacggca
     5101 ctcgctcgcg agcaggcgat tcaaatcgag gacctgcttc ggtgcggcgt tttgctggcc
     5161 gagggtgagc agatcttgaa tggtgtcggc ggcgcgtagg ccggcgaggc gcagcgtctc
     5221 gatgtcctcg aaaatgccgg gcggagccgc gtcgccgatc gcgcgctgga gctcgctcga
     5281 gatggtttcg ggaagaccca cgagcggacc gagcacgttg ttcaggtcgt gagcgacgcc
     5341 gcccgcgacg agactgagcg aacgcaggcg ctccgcggtg acgcgcttct cctcctccgc
     5401 ttgcgcgctc agccgggcct gttggaggat gtcctcgtgc aagcgcatcg tgcgcacggc
     5461 gctgccgatt tgctctcgca agacggcgta aagctcgtgg ctcttcggca gctcgagcac
     5521 cgcgatcccg aggttctgcc actcgaaggt gagggggacg accgcgagcg agcagcgctt
     5581 gctcgtcatc agcgccccgt gcggtaagag caagctcgct gggtacgact gctctttcag
     5641 cgcgactggc tcgccgttca tcacgcagac gagcggctgg agcgagtcca gatcgtcgcg
     5701 ggcataaact cccacgaacg cgttgcagat ggacaagctc ggcagctgaa tctcgaggca
     5761 gcttcgcagg gacggaaggt cgaaggcgct cgccgacaag cggtcccaat tcgcgcgcag
     5821 ccgctcggtg aggtcgtcgt cgtggacgcg acgcttctct tcgcggcggt gcattgcttg
     5881 gccgaggtcg accagggtgc ggcgcatggc ggtctcgagc tctcgcgtca cctcctgctc
     5941 ggggacggcg tcgcgcagct gaacgatgac gtgctgcagt tcatggacgg gcgcttcgtc
     6001 tccgaggcgc tcgagcaacg ctcgcaccgt gcgcgagaac gagcccgcga gctcgcctcg
     6061 ctcacccttc agcgagcgac acagcgctcg cgcccactcc tcgcggcgcg cgcggtcgcc
     6121 gatgagcggc gtcagcgatt cggcgatgcg ctcttcggag ccgctcgcgg cagactcttc
     6181 cgaccacaag tcgattcccc cacagccaca cgactcgcgg aacaccgctt cggtcggtaa
     6241 cgtcaggagc tccacgtcgg gcttgccttc ccacgccctg agcactcgcg cgacggcgct
     6301 cgcgccgagc tccacgatgg gttgccgcac ggtcgtcagt ggtggagacg ccgcgcgcga
     6361 gctcgccgca tcgtcgaagc cgccgatgag cacgtcttgc gggcagcgca accccgcccc
     6421 cttcaaggcg ccgagcgcgc ccaccgccat gtcgtcgttc gcggcgatga gggcgtcgaa
     6481 atgcacgcct cggcgcaaga attctttggc cgcgtcctca cccgcgctga acgcgaagtt
     6541 cgcgtggata accgacgtgg cagggagctc gagcccatgt tcggcgagcg cgctgcgcgt
     6601 tcctctcagg cgagcttccg attcgttgtg ccctgcgggg ccggcgacgt agacgaaacg
     6661 ccgcgcgccg tgcgcgctcg cgaagtggcg ggccagttgc gctgaaccag atgcattgtc
     6721 gacgacgacg ctcgcgacgg ttggacaatg caaaccgact gcgcacgtcg gcacgatgct
     6781 gcggcgcgtg acgagctcga gcaccgtctc ttccgagagc gccgccgcaa tgcagcttgc
     6841 cgccaccacg accccgtcga cgcggttcgg cgacaccatc tcgtaaacgc gggcctgcgc
     6901 ggctccccac gatcgccagt cgctgcgacc cgcgtacacc cagaggtcga tatcgcgttc
     6961 gatacacgcc tgctcgatgc cctggcgcag aaggccctgg tagtccgagc gcatgccggc
     7021 gttgttggtc aaaaatgcga ttactttgcg ccgggacatc gagttgctcg cggcgccttc
     7081 ggcgagacgc gggcgacact ccgaaccccg caccggcagc gcgaaccagc ttccatcgaa
     7141 tacgtcatct gcgccgcgtc gtgaagggag tcgttcgacg ccctttcacg gcgtttgcct
     7201 actcgcgccc acttcgcccg acgctggcgc gcggggctgg tatcacgccg gaaaatcggg
     7261 caaacgaggg gcatggccac cgtggagatc acgaagcacc tctgggagtt cctgcccgta
     7321 ttgcgtgaca agacgttgaa cgtagaggcc agcaccgtcg cggaggtgat ccgcgaggtg
     7381 gagcgcatcg cgcccgggtt cacgtattac atctgcgacg agcggggctg ccttcgtacg
     7441 cacgtcaaca tcttcgttgg tcaagagcgt atttgcgatc gcactcgctt gagcgacccc
     7501 gtaaacgacg actcgcgcgt gctcatcatg caagccctga gcggcggctg accgagaagc
     7561 acagcgcgcg tacgaaaggg ttgcagcgcg agctggttcg aggctagagc cgagcgatgc
     7621 gcgttctcgt tggcactcga aagggcacgt tcttcgtcga aaagcaggct gggagctgga
     7681 agcctcgcct cgtaggccac cgcggtgtcg gcgtgaactt cgtcgcccgc gatccgcatc
     7741 gcggcacgct gtgggcagcg ctcgggcacg ggcactgggg cgcgaagctt tcgcgctcgg
     7801 acgacgacgg cgc
//
LOCUS       DASTKS010000238         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1600578, whole genome shotgun sequence.
ACCESSION   DASTKS010000238 DASTKS010000000
VERSION     DASTKS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600578"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFQ35_04975"
     CDS             <1..349
                     /locus_tag="VFQ35_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET9930015.1"
                     /translation="VVDVRASSRLLDSPACLVQPEGTLPPHIERLLRAQKRDVPGARR
                     ILELNLSHPLVKSVTTLDEREPSSERVREWLELLHDQALLAEGSPIEDPAGFAKKLTR
                     LLTRAAESEISAP"
     gene            482..1474
                     /locus_tag="VFQ35_04980"
     CDS             482..1474
                     /locus_tag="VFQ35_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9930016.1"
                     /translation="MKVLFVGGTGIISSACSALAVERGIELHLLLRGKTAHRSPPAGA
                     RLLHADIHDPRAVDSALGNARFDAVVNFIAFTPEHVQRDVELFKGRTQQYVFISSASA
                     YQTPPARLPVTESTPLFNPHWQYSRNKIACEEVLVKAYREQGFPMTIVRPSHTYDKTL
                     LPAHGGYTVIDRMRRGKPVIVHGDGSSLWTLTHHRDFARAFVPLLGHDGALGEAIHIT
                     SDEAISWDRIHELMAHAAGAEARIVHVPSDLIAGYDAEWGASLLGDKTHSMIFDNTKV
                     KRLVPEFRAEIPFCRGIQEILAYYDGDPSRRVVDHEVDELMDRIIEAQNSIHPV"
     gene            complement(1602..>2089)
                     /locus_tag="VFQ35_04985"
     CDS             complement(1602..>2089)
                     /locus_tag="VFQ35_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930017.1"
                     /translation="PPPPAPEPTRRQAPSAMLTARDVAFLVEYGSSDARERADADCEK
                     ESKGDAAAQAACMSKAREKFLPDVLRFLKDDKGKVTLLVYKRTGSSLREVYLGAVEFK
                     NESEDKVQLRFTGREKGTRPIFRRAEPTISVPNDYSIEIDDGELGKLTYSAKIGLVAS
                     P"
BASE COUNT          337 a          714 c          669 g          369 t
ORIGIN      
        1 agtggtggac gtgcgtgcgt cgagtcgcct cctcgattcg ccggcgtgcc tcgtccagcc
       61 cgaaggcacc ctgccgccgc acatcgagcg tctgctccgc gcccaaaagc gcgacgtacc
      121 gggggcgcga cgcatcctcg agctgaacct ttcgcatccg ctggtaaaga gcgtgacgac
      181 gctcgacgaa cgcgagccga gctccgagcg cgtgcgcgaa tggctcgagc tcttgcacga
      241 ccaggcgctg ctcgccgagg gcagcccgat cgaagacccc gccggcttcg cgaagaagct
      301 cacccgcctt ttgacgcgcg cggccgagag cgaaatctcg gcgccttagc cggcgttgaa
      361 cgccccgcta aggcgttctt gctcaggctc tttgcaattc ttctcgacag cgcctgagcg
      421 ccaatccgac tgacgaactt tcgtggaaac gggctacgct gcgcggcgag gtacgtagcc
      481 catgaaagtt ctgttcgtcg gtggcacggg aatcatcagc tcggcatgct cggcgctcgc
      541 cgtcgagcgc ggcatcgagc ttcacctctt gctgcggggc aagaccgcgc atcgctcgcc
      601 gccggcgggg gcgcgcctgt tgcacgcgga cattcacgat ccgcgcgcgg tcgattcggc
      661 gctcggcaac gcgcgcttcg acgcggtcgt gaatttcatc gcgttcacgc cggagcacgt
      721 ccagcgcgac gtcgagctgt tcaagggccg cacgcagcag tacgtcttca tcagctcggc
      781 ctcggcgtac cagacgccgc ccgcgcgcct gccggtgacc gagtcgaccc cgctcttcaa
      841 cccgcactgg cagtattcac gcaacaaaat cgcgtgcgaa gaggtgctcg tcaaagcgta
      901 ccgggagcaa ggcttcccga tgacgatcgt gcggccgtcg cacacgtacg acaagacgct
      961 gctcccggcg cacggcggct acaccgtcat cgatcgcatg cgccgtggca agccggtcat
     1021 cgtgcatggc gacgggagct cactctggac gttgacgcac caccgcgact tcgcgcgcgc
     1081 cttcgtcccg ctgctcggcc acgacggcgc gctcggtgaa gccattcaca tcacctccga
     1141 cgaagcgatc agctgggatc gcatccacga gctcatggcc cacgccgccg gcgccgaggc
     1201 gcgcatcgtg cacgtcccct cggatctcat cgcgggttac gacgcagagt ggggcgcgag
     1261 cctgctcggc gacaagacgc acagcatgat cttcgacaac accaaggtaa agcgcctggt
     1321 tcccgagttc cgcgccgaaa tccctttctg ccgcggcatt caagagatcc tggcgtacta
     1381 cgacggcgac ccgtcacgcc gcgtcgtcga tcacgaagtc gatgagctca tggatcgcat
     1441 catcgaggcg cagaactcga tccatcccgt ttgatggggc aatgcaggca gcccggcgcg
     1501 ggaacgcgcg agcaacggcg tggtgcgatc gattttggtt ggttgcccac gccgcgcggc
     1561 gccgttgcgc gttccgagcc cactgcggcg cgtcgcttcc gttacggtga cgcaaccagc
     1621 ccgattttgg cgctgtaggt gagctttccg agctcgccgt cgtcgatttc gatgctgtag
     1681 tcgttgggca cgctgatggt cggctcggcg cggcggaaga tcgggcgcgt gcccttctcc
     1741 cgaccggtga agcgaagctg taccttgtct tcgctctcgt tcttgaactc gaccgcgccc
     1801 aggtagactt cgcgcagtga cgagcccgtt cgcttgtaga cgagcagcgt gaccttgccc
     1861 ttgtcgtctt tgagaaagcg gagcacgtcg ggcaaaaact tttcgcgcgc cttgctcatg
     1921 cacgcagcct gcgcggccgc gtcgcccttc gactccttct cgcagtcggc gtcggcgcgt
     1981 tcgcgcgcat cggagcttcc gtactcgacg aggaacgcga cgtcgcgcgc ggtgagcatc
     2041 gcgctcgggg cttgacggcg cgtcggctcg ggtgcgggcg gcggcggcg
//
LOCUS       DASTKS010000239         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1600619, whole genome shotgun sequence.
ACCESSION   DASTKS010000239 DASTKS010000000
VERSION     DASTKS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600619"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFQ35_04990"
     CDS             <1..859
                     /locus_tag="VFQ35_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930018.1"
                     /translation="MPSLDHELLIELFRNQPELAAQLLRDALHLELPAYTEARLASSD
                     LTELVPTEFRADAVVLLVDGKPVLGVIVEAQLSRDERKRFTWPAYVSVLRARHQCPVE
                     LLVVTPDRAVATWASTPISLDLTGSAALRPRVLGPETIPIVTDAERAAREPEFAVLSL
                     MAHGRDDTETAVSIALAAGHAVQFLSEDQRVLYLALIESALSDEAKKAFEMHPQSGKL
                     ISSWQRAPFEKARAAEKAADIIAVLEARGLVVSDAQRQRITETSDLDTLSRWVRRAAT
                     VTSTDALFE"
     gene            complement(1381..>2321)
                     /gene="xerC"
                     /locus_tag="VFQ35_04995"
     CDS             complement(1381..>2321)
                     /gene="xerC"
                     /locus_tag="VFQ35_04995"
                     /inference="COORDINATES: protein motif:HMM:NF002331.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerC"
                     /protein_id="HET9930019.1"
                     /translation="ERYQRWLFHFRREDGRALSFQTQHKRLVQVRMYFKWLTRQNVLL
                     WNPASELELPKLERRLPRHVLTETEAEKVMLQPDTTEPMGIRDRAILEVLYSTGIRRM
                     EVVNLRVFDVDGERGTLIVRQGKGKKDRTVPVGERAALWVEKYLTEVRPELVMPPDEG
                     VLFLTEDGRSLPANRLTQLVREYVTAAETGKKGACHLFRHTMATVMLENGADIRYIQE
                     MLGHVELSTTQIYTQVSIRQLKAVHALTHPSAKLERLRRAERGSAPTLPMVATPDGDD
                     ALARPELAKTVSTREELFSLLAAEAEEERLAEDGSN"
BASE COUNT          380 a          846 c          680 g          415 t
ORIGIN      
        1 gatgccctcg ctcgaccacg agttgctcat cgagctcttt cgcaatcagc ccgagctagc
       61 cgctcagctt ctgcgggatg cgcttcacct cgagctgccc gcctacaccg aggcgcgcct
      121 cgcttcctcc gatctcaccg agctcgtgcc caccgagttc cgcgcagacg cggtcgtcct
      181 gctcgtcgat ggcaagcccg tcctgggcgt tatcgtcgaa gctcagctct cgcgcgacga
      241 gcgcaagcga ttcacgtggc ccgcttacgt ctccgtcctt cgcgctcgtc accaatgccc
      301 cgtcgagctc ctggtggtga cgcccgatcg cgcagtcgcc acttgggctt cgactccaat
      361 cagtctcgat ttgacgggct ccgcggcgct tcgtcctcga gtgctcggtc ccgagacgat
      421 cccgatcgtc accgatgccg agcgtgcagc gcgcgagccc gagttcgccg tgctctcgct
      481 catggcgcac ggcagagacg acaccgaaac cgctgtttcg atcgcactgg ctgccggtca
      541 cgccgtccag ttcctgtccg aggaccagcg ggtgctatac ttggccctca tcgagtccgc
      601 gctcagcgac gaagcaaaaa aggccttcga aatgcacccc caatcaggaa aactcatcag
      661 cagttggcaa cgtgcaccct tcgagaaggc tcgcgctgcc gagaaggctg cggacatcat
      721 cgctgttctc gaagcccgag gcctcgtcgt ttccgacgca caacgacagc gcatcactga
      781 aacctccgat ctcgacacgc tctcccgttg ggttcggcgc gctgcaacgg tcacttcgac
      841 tgacgccctg ttcgagtgag ccgcgccgtc cggtggtggg ggcactgacg cactgaggcc
      901 gcgggtgggg cggggccaca cgcggccgag tcgctcagcg cgggggcggg tgggcaagcg
      961 acgtccagcg ccgtaacgcc cagcgccgcg cctgagcacg gtgtcgacgg cgcatgcgcc
     1021 gtccgtccca ttcacgtcgc tattcgccgc tggcgccggc gacacggtgc gcgccgctca
     1081 gcgcgcccaa gccgcacagc gacgcgcgtc ccttgagcgc agccggcccg gcggaggaga
     1141 cataacgttc tgttatgtct aggtcggctc gggaagcacg cgggccgggg cgtcgcttcg
     1201 cattgtgcga cctcgagcca gtagccccgg cacgcgggcc gcttcgcata acccgggtta
     1261 tgcgaggcgc cgagacgacc gactccgccg ggcgggcgga gcgtctctgc cgctcagcgc
     1321 gagccgcaaa gcgcggtcca acgccgtgct gccgcacaac gacgatcagc gcgcaggcgc
     1381 tcagttcgag ccgtcttcag cgagccgttc ctcttcggcc tcggcagcta aaagagaaaa
     1441 gagctcctcc cgcgtactca ccgtcttcgc gagctcgggc ctcgccaatg cgtcgtctcc
     1501 gtcaggggta gcgaccatcg ggagcgtagg ggcggagccc ctctctgccc gccgcaggcg
     1561 ttcgagcttg gcggacggat gcgtcagcgc gtgcaccgcc ttcagctgcc gaatcgatac
     1621 ctgcgtgtaa atctgcgtcg tcgacagctc cacgtgcccc agcatctctt ggatgtaccg
     1681 gatgtctgcg ccgttctcga gcatcaccgt cgccatcgtg tgccgaaaca agtggcacgc
     1741 gcccttcttc cccgtctccg ccgccgtcac gtactcgcgc accagctgcg tcagtcggtt
     1801 cgctggcagc gagcgaccat cctccgtcaa aaacaacacg ccttcatccg gcggcatcac
     1861 gagctcgggc cgcacctccg tgagatactt ctccacccac agagcggcgc gctcgccaac
     1921 cggcaccgtc cgatctttct tgcctttgcc ctgcctcacg atcagcgtcc cgcgctcgcc
     1981 atcgacgtcg aagactcgga ggttcacgac ctccatccgc cggatccccg tcgagtaaag
     2041 cacctctaaa atggctcgat cccgaattcc catcggctcc gtcgtgtccg gctgcagcat
     2101 caccttttcc gcttccgtct ccgtcagcac atgccgcggt aagcgccgct caagcttcgg
     2161 gagttcgagc tcgctcgctg gattccagag aaggacgttc tggcgcgtca gccacttgaa
     2221 atacatccgc acctgcacga gccgcttgtg ctgcgtctga aagctcagcg ctcgcccatc
     2281 ctcgcgccga aaatgaaaca gccatcgctg gtaccgctcc a
//
LOCUS       DASTKS010000240         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1377312, whole genome shotgun sequence.
ACCESSION   DASTKS010000240 DASTKS010000000
VERSION     DASTKS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377312"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFQ35_05000"
     CDS             complement(<1..368)
                     /locus_tag="VFQ35_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9930020.1"
                     /translation="MAKEKFVRTKPHVNVGTIGHIDHGKTTLTAALVKVQSKKNLAKE
                     IKYSDIAKGGIVRDETKTVTIAVSHVEYETATRHYAHVDCPGHADYIKNMITGAAQMD
                     GAILVVSALDSVMPQTREHVL"
     gene            complement(431..503)
                     /locus_tag="VFQ35_05005"
     tRNA            complement(431..503)
                     /locus_tag="VFQ35_05005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(468..470),aa:Thr,seq:ggt)
     gene            complement(607..679)
                     /locus_tag="VFQ35_05010"
     tRNA            complement(607..679)
                     /locus_tag="VFQ35_05010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:Gly,seq:tcc)
     gene            complement(820..902)
                     /locus_tag="VFQ35_05015"
     tRNA            complement(820..902)
                     /locus_tag="VFQ35_05015"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(866..868),aa:Tyr,seq:gta)
     gene            complement(919..991)
                     /locus_tag="VFQ35_05020"
     tRNA            complement(919..991)
                     /locus_tag="VFQ35_05020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(956..958),aa:Thr,seq:tgt)
     gene            complement(1718..>2812)
                     /locus_tag="VFQ35_05025"
     CDS             complement(1718..>2812)
                     /locus_tag="VFQ35_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930021.1"
                     /translation="EGAKVPRTENIEVPRTIVTPGDAKSIPELYAQASKLAASGEHAR
                     AAREFARVAALDPNGELADDALFQSASEHDEASEFGDAAATYRELARRYPKSEFRSRA
                     MLSATRLYAHLEQYREAGELAEHLLAEKQELGPFALVVLYGASALSRLDAGDEKSAAD
                     YIEKGREIIDARQLDAAGQISRDLSSLYFALGELRRRRAERIVFQPTPPNFGAVLEQR
                     CQLLLDAESAYSDAMRAYDAHWSTMAGFRVGELYEKLHEDLMKVTPPASADTVQKRQL
                     FEGAMRLRYAILLEKALKMMEHTLAMAARTGEHSGWVAKSEQAKKAIEQAMKREDAAL
                     AALPYSRGTLQMALDMLEKKHSSESANPYK"
BASE COUNT          458 a          968 c          828 g          558 t
ORIGIN      
        1 agaacgtgct cacgggtctg cggcatcacg ctgtccagcg cgctcaccac caggatcgcg
       61 ccgtccatct gcgccgcgcc cgtgatcatg ttcttgatgt agtcggcgtg gccggggcag
      121 tcgacgtgcg cgtagtgacg cgtcgccgtc tcgtactcca cgtggctcac cgcaatcgtc
      181 accgtcttcg tctcgtcgcg aacgatgccg cccttggcga tgtcggagta cttgatctcc
      241 ttcgccaggt tcttcttcga ctgaacctta accagggccg cggtcagagt cgtcttgccg
      301 tgatcgatgt gtccgatcgt cccgacgttc acgtggggct tggtgcgaac gaatttctcc
      361 ttggccatgg caacctttca ggatctactt gtcgatgttc ggtacgatga gagaagaggg
      421 gatttgctcg agcccacaac caggattgaa ctggtgacct cgtccttacc aaggacgcgc
      481 tctgccatct gagctatgtg ggcaaaggga atccgagggg ggcggagcct tgcacgaagc
      541 tcgcgcattg caaccccctt accgcttggt tccgaattgc cgcgaggctg aaacgaagcg
      601 acttcgagcg ggagaaggga ttcgaaccct cgacgttcag cttggaaggc tgacgctcta
      661 ccaactgagc tactcccgcg atggactccc aacgcgcacc acggcttggc gtgctttcga
      721 gtgactgccg ggctgcaccc ggactggcca accgaatcac tccgccgaac cttgaacgcg
      781 cgctccgaca gcggcctcga accgagacca ctgaacgggt ggagggtgtt ggattcgaac
      841 caacgtaggc tgacgccggc aggtttacag cctgctccct ttggccgctc gggcaaccct
      901 ccatgacttt cgtcttagag ctagcgaggg gacttgaacc cctaaccacc tgtttacaaa
      961 acaggtgctc taccgattga gctacgccag ccaagcctcg acggatggac ctttcgctca
     1021 cgacccgtga ggggcggcct ttagacgaca ctcttagaga gccgttagcc tgcatgagca
     1081 agttccgacg gagaatcgat gcggagccgg caagcgccgc agagtgcgac gccgctcaaa
     1141 aggggcaccc cacctgtcct ccagccgccc cagccggcgc gcgggccgat aagacagtaa
     1201 aggattagtg gggaccgacg aggaccgcgc tctggtagcg cctggtctca ggggtgtcaa
     1261 gcatatttca taccgactcg tgggcggctt tgccccacgg gccagctgac gccgcttttc
     1321 caataaattc cgccgactta atgcgcgctt tcttcgccac ggcttgaacc agaatcgcga
     1381 gaattttttg gggagtgggt tggcccgcag cgctcgactg tgcgcgcggc ccgcgcccac
     1441 tgcacgcttc gtcctcttcg tccccttcgt cccccgcccg acagcgccgc ttcctctctc
     1501 ttccccgccc cgcgccaatg cgagcggatt cgtctccgcg ccccgcgcca ctgtgcacgg
     1561 ttctggtgtt ccccgctccc ctcgccgacg cgcgtcgctt cgtctccgcg ccccgcgcca
     1621 ctgcgcacgt cgttctagtg tttccccgcc ccgcgccgac gcgagcggat tcgtctccgc
     1681 gcccccgcgc cgtgcgcgcg tcgttctcgc ggtcgttcta tttgtacggg ttggcgcttt
     1741 cgctcgagtg cttcttctcc agcatgtcga gcgccatctg cagtgtgccg cgcgaatacg
     1801 gcagcgctgc gagcgctgcg tcctctcgct tcatcgcttg ctcgatggcc ttcttcgctt
     1861 gctcggattt cgcgacccac cccgagtgct cgccggtgcg cgccgccatc gcgagcgtgt
     1921 gctccatcat cttcagcgcc ttctcgagca gaatcgcgta acgcaatcgc atcgcgcctt
     1981 cgaacagctg gcgcttttga acggtgtcgg cgctcgcggg cggcgtcacc ttcatcaagt
     2041 cctcgtgcag cttttcgtag agttcgccga cgcgaaagcc cgccatcgtc gaccaatgtg
     2101 catcgtacgc gcgcatcgcg tccgagtagg cactctcagc gtcgagcaaa agctgacagc
     2161 gctgctcgag caccgcgccg aagttcggcg gcgttggttg aaacacgatg cgctcggcgc
     2221 gccgtcgccg aagctctccg agcgcgaagt acaaagacga aaggtcccgc gagatctgcc
     2281 cggccgcgtc gagctgacgc gcgtcgatga tctcgcgacc cttctcgatg taatcggcgg
     2341 cgctcttttc gtcgcccgcg tcgagccgtg aaagcgcgct cgccccgtac aacacgacga
     2401 gcgcgaaagg cccgagctcc tgcttctccg cgagcagatg ctccgcgagc tcgcccgcct
     2461 cgcgatactg ctcgagatgc gcgtagagac gcgtcgcgct gagcatcgcg cggcttcgaa
     2521 actcgctctt cggatagcgc cgcgcgagct cgcgatacgt cgcggcggcg tcgccgaact
     2581 cgctcgcctc gtcgtgctcc gacgcagact gaaacagcgc atcgtcggcg agctcgccat
     2641 tcgggtcgag cgccgccacc cgcgcgaact cgcgagccgc gcgcgcatgc tcgccactcg
     2701 ccgcaagctt actcgcttgc gcatagagct cgggaatact cttcgcgtca ccgggcgtta
     2761 cgatcgtgcg tggcacctcg atgttctcgg tacgcggaac cttcgcgccc tc
//
LOCUS       DASTKS010000241         7135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_485614, whole genome shotgun sequence.
ACCESSION   DASTKS010000241 DASTKS010000000
VERSION     DASTKS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485614"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..844
                     /locus_tag="VFQ35_05030"
     CDS             110..844
                     /locus_tag="VFQ35_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930022.1"
                     /translation="MRHPVKSSAGLAIALFVAVSCNGLVGNDPPYLVGGASGTVNEAG
                     AGADEGAGQSGGGLTSGGSSSRGGARSGGGSPSGGGRATGGASTTGGAGGDAGAAGAG
                     GSSPCPGECMPGTVDRPMQKCGVCSKGTQTRQRTCGSDCRWGAYSAWSTCALGTVECM
                     PGAVDSDSQQCGACNKGSQSRSRTCGDQCTWGAWGAWGACAEKAQCKPDHYDCCGTNK
                     WKWCLPDTCTWSDQCESCSSPNCDCP"
     gene            860..1639
                     /locus_tag="VFQ35_05035"
     CDS             860..1639
                     /locus_tag="VFQ35_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9930023.1"
                     /translation="MLLYKTFLWTHSYLRWLIVIATAILIVATARGWRRGSKFSTPHE
                     RLQRAVLGLVDTQFLLGLALFLWLSPISKGFFTAPAIGMKDSALRFFGMEHPLGMLLA
                     IGVLHGMHRRSRLAPSDAVRHRRACLGAVSFLVLAVASIPWPGTAHGRPLFRRPEAAS
                     GAAPSAVEPSCPSTYGARCAACHGDRGRGDGMVGRYLKPPARNFAEPGFLQARSRAGI
                     RAVIRDGGAAHGLSAAMPAHVDLSEQEVDALVACVQSLGQR"
     gene            1692..3071
                     /locus_tag="VFQ35_05040"
     CDS             1692..3071
                     /locus_tag="VFQ35_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930024.1"
                     /translation="MQSVAPAFARLRRFRPAYAALSLGLGSALLLPGNARASNEDDFF
                     LGNRAAMTAGAVTASVGDGSAAFNNPAGLASADRSSIDVSGSVYCLRFYRASHFLGLD
                     GGPSKDASVTEFLAIPTAIAYLTRLADGVSLGLGYFVPRSSDFVLRENLDVNDGKQSS
                     NFGLNLRQSRSDYLLIAALGARVTPRLRVGVSVAAALENVVSAAALFGSVSRGLDTQK
                     SLQSDEFSTRWAVGMELDVGAQWDVTPALTLGVALRGPRLRVWQTVDGRTNTSAAAVE
                     PGLDPILLTGSTAVEPNGNTVSLVRAGRYIAGMALQLGAGTLSVDADVQPGLRLPGAG
                     VDRRLTFNARVGYVYEITPHLLLGAGVFSDRATEPQGFSLVGAQGDFYGGTLGLEINN
                     VHRLAPGDEAQSMQLSTVFAFRYATSSGSTTKLRAHPDQQVPLLFEEARGGIAVHEIG
                     FHVGSALHF"
     gene            complement(3110..4690)
                     /locus_tag="VFQ35_05045"
     CDS             complement(3110..4690)
                     /locus_tag="VFQ35_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9930025.1"
                     /translation="MTTSTASLDALAVPDKTRRWLIAVSVALGALLEIVDTSIVNVAL
                     PQMQASLGVTLSEVGWVVTSYAIANVIILPLSAWLGDRFGKKRFFVFSLIAFVGASIL
                     CGLSHSLPMLVLSRVLQGLFGGGLLSKAQSFLWETFPPEERGSAQALFGVVAIAGPAI
                     GPTLGGYIVTNLDWRWIFFINLPIGVLAVFMAATFLPDDRPTQSSGSVDWVSLGFLAA
                     GLGALQTFLEEGYSDSWFDSTFICICAALAVICLVAFVHRQLTTESPVVDLRVLRYRS
                     LWAGCIVSAVIGMGLYGAMFAVPIFAQTVMRYTSQQTGMLMLPGALASAFAMPVMARL
                     LRRFDPRVLVAAGSIIVITALMMLAQLNPQTNESELFWPLIIRGFGTVAMFLPMSMAT
                     FASVPVKDVAKATGLYNLTRQLGGSIGIAVLTTVLSSRTAFHRSVLVEKLGISDVGTL
                     ERVRAIAGSFMAKGFDAESARRQALTLLDGNVNLQASVMSFADSFWLVAVVFLCTMPL
                     LFLLGGKAKGRAAVADAH"
     gene            complement(4701..5996)
                     /locus_tag="VFQ35_05050"
     CDS             complement(4701..5996)
                     /locus_tag="VFQ35_05050"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET9930026.1"
                     /translation="MSAQSVAKHPATADSVPPRSQASSAAVGPAAPAPAEAPAKPAGK
                     GRMIMLGLLAALVVGGAGYWLQHRGLESTDDAQLDADVVAVPARVGGTVTALHFADNQ
                     RVKAGELLIELDDAQAKAKFAEAEAELLAAKASADAAEATAELTEKNVSAGQNIARAS
                     LSGAAASMTASADQIAEAKANLASATTLREKAKLDLERTQRLAQMNAISRAELDSAQA
                     SFDTAQSAMDQAQARIVTLQSSATQARARVTEASARLGQASDTQTAQIAEARAKATSA
                     RARVATAEAMRDLARLDLSYTKIFAPRDGIVSKRSVAVGQLVSPGQSVVMVVPDARAG
                     GPVWVTGNFKETQLAHMHVGQPATLTIDAYGRELHGHVESFSGATGARFALLPPDNAT
                     GNFTKVVQRVPVRVKLDDAPGDMMLLPGLSVELSVNTRL"
     gene            6109..6750
                     /locus_tag="VFQ35_05055"
     CDS             6109..6750
                     /locus_tag="VFQ35_05055"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9930027.1"
                     /translation="MKNQEAEQSQSLSSAAGPAARRTGGRSARVVRDVLAAALEVFAE
                     QGYAGFSIDDVATRASVNKTTVYRRWPTKADLVGAALISLRDQDPEPPDTGSLRADLV
                     ALLHQRATQMGTPARRAIMQALLVSNSEPELLALTQKLRRERPGIPRVLFQRAVERGE
                     LPADVDVALMTEALFGAVLSRTWKRERVSHSFLTRLVDLVVAGAAAGAGRLRS"
BASE COUNT         1196 a         2319 c         2412 g         1208 t
ORIGIN      
        1 aatcgggctc ggctaggcga ggctccacga gagcctgacg tttcccgccg ggtcgtgcgt
       61 gccgacgcgt cgcgtggttc aatgcgcccg gagccggcga ggtagaagca tgcgtcatcc
      121 tgtgaagagt tcggcggggc ttgcgattgc cttgttcgtc gccgtgtcgt gcaacggact
      181 cgtgggcaac gacccgccgt acctcgtggg cggagcgagc ggaaccgtca acgaagcagg
      241 cgcgggcgcg gacgaaggcg ccggccagag cggcggaggc ctcacgagcg gcggctcctc
      301 atcgagaggc ggcgctcggt cgggcggggg atccccgtcg gggggcggtc gcgcgaccgg
      361 gggcgcgtcc accacgggcg gtgccggcgg cgatgccggt gcggccggtg ctggggggag
      421 ctcgccgtgc ccgggtgagt gcatgcccgg cacggtagat cgcccgatgc agaagtgcgg
      481 agtctgcagc aagggcacgc agacgcgtca gcggacctgc ggcagtgact gccgctgggg
      541 cgcttacagc gcttggagca cttgtgcact cggcaccgtg gaatgcatgc ccggcgcggt
      601 cgactccgac tcgcagcaat gcggcgcctg caacaaggga agccagtctc gttcgcgaac
      661 ttgcggcgac caatgcacct ggggcgcgtg gggggcctgg ggcgcgtgcg cggagaaggc
      721 gcaatgcaag ccggaccact acgattgctg cggaaccaac aagtggaagt ggtgcttgcc
      781 cgacacttgc acgtggtcgg atcagtgcga gtcatgcagc tcgccgaact gcgactgccc
      841 ctgagtcgcc ccgcgccaca tgctgcttta caagactttt ctgtggaccc actcctatct
      901 gcgttggctc atcgtcatcg cgaccgcgat tctgatcgtc gcgacggcgc gcggatggcg
      961 gcgcggaagt aaattttcga caccccacga gcgcctgcag cgagcagtac tcgggctcgt
     1021 cgacacccag ttcttgttgg ggctcgcgct cttcctatgg ctgagcccca tctcgaaagg
     1081 tttcttcact gcgcccgcga tcggcatgaa ggactcggcg ctccgtttct tcggaatgga
     1141 gcatccattg ggcatgctgc tcgcaatcgg cgtgctgcac gggatgcatc ggcgctcgcg
     1201 ccttgctccg agcgatgcgg ttcgccatcg ccgagcctgt ctcggggcgg tctccttcct
     1261 cgtcctcgcg gtcgcctcga tcccgtggcc cggaactgcg cacggccgcc cactatttcg
     1321 gcgccccgag gcggcaagcg gagccgcgcc atccgccgtc gagccgtctt gtccgagcac
     1381 gtatggggcg cgctgtgcgg catgccatgg cgatcgcggc cgcggagacg gcatggtggg
     1441 ccgctacttg aagcccccgg cgcgaaactt cgccgagcct gggtttttgc aggctcgtag
     1501 ccgcgcaggg attcgggctg tgatccggga cgggggcgcc gcgcacggcc tgagcgccgc
     1561 gatgcccgcc cacgtcgact tgtccgagca agaagtcgac gcccttgtgg cgtgcgtgca
     1621 gagcctcggt cagcgctaaa ttcgcgccgt gaggcccggc gagcgcgccc tggcgcgtgt
     1681 atgctgcgca cgtgcaatcg gtcgcgcccg catttgcgcg cctacgtagg tttcggcctg
     1741 cgtatgccgc gctgtctctc ggtctcggga gcgcgctact gctcccgggg aacgcgcgcg
     1801 ccagcaacga ggacgacttc ttcttgggca atcgcgccgc catgacggcg ggagccgtga
     1861 ccgcgagcgt cggtgacggc agcgccgcgt tcaataaccc ggcagggttg gcgagcgccg
     1921 accgcagcag catcgacgtg agcggcagcg tgtactgcct tcgcttctat cgggcgtcgc
     1981 acttcctcgg actcgacgga ggaccttcca aagacgcgag cgtgaccgag tttctcgcga
     2041 tccccaccgc gatcgcctat ttgacgcgtc ttgcggacgg cgtttcgctc ggtttgggat
     2101 acttcgtccc gcgctcgagc gatttcgtgt tgcgcgaaaa cctcgacgtc aacgatggca
     2161 agcagagctc gaacttcggc ctgaacttgc gccagagccg cagcgactac ctgctcatcg
     2221 cggcgctcgg cgcgcgcgtg acgccccgcc tccgagtcgg ggtcagcgtc gcggcggcgc
     2281 tcgaaaatgt ggtgagcgcg gccgcgttgt tcggctccgt gtcgcgcggg ctcgatacgc
     2341 aaaagtcgct tcaaagcgac gagttcagca cgcgctgggc ggtcgggatg gagctcgacg
     2401 tcggagctca atgggacgtt accccagcgc tcacactcgg ggtcgcgctg agaggtcccc
     2461 ggttgagagt ctggcaaacg gtggatggcc gaaccaacac ctccgcggcc gcggtcgagc
     2521 cagggctcga tcccatcctg ctgacgggct caaccgcggt cgagccgaat ggaaacaccg
     2581 tcagtctcgt gcgggcgggt cgatacattg cggggatggc tcttcaactc ggcgccggca
     2641 ccctcagcgt ggacgccgac gtgcagcccg ggctgcgcct gcccggtgcg ggcgtcgatc
     2701 ggcgtctgac tttcaacgcg cgcgtgggct acgtttacga gatcacgccg cacctattgc
     2761 tcggggcagg cgtcttcagt gatcgagcga cggagccgca ggggttcagc ctcgtcggcg
     2821 cgcagggtga tttctacggc ggcacgctcg gcctcgagat caacaacgtg catcggttgg
     2881 cgcccggcga cgaggctcag tcgatgcagc tgtcgaccgt cttcgctttc cgctacgcga
     2941 cgagctcggg cagcacgacc aagctgcgcg cgcacccaga ccaacaggtg ccgctcttgt
     3001 tcgaagaggc tcgcggcgga atcgccgtcc acgagatcgg cttccacgtg gggtccgcat
     3061 tgcacttctg atcagggccg gactcgcccc ggcgcgctcc ctcgcgttct cagtgggcgt
     3121 ccgccaccgc cgcgcggccc ttggctttgc ctccgagcag aaacaagagc ggcatggtgc
     3181 agaggaacac caccgcgacg agccagaacg aatcggcgaa cgacatcacg gacgcttgaa
     3241 ggttgacgtt gccgtccagc aacgtgagcg cctgacgacg cgccgactcc gcgtcgaacc
     3301 ctttcgccat gaacgagccg gcgatggcgc ggacccgctc gagcgtgccg acgtcgctga
     3361 ttcccagctt ttcgacgagc accgagcgat ggaacgcggt gcgtgacgac agcaccgtcg
     3421 tgagcacggc gatgccgatg ctaccgccga gctggcgcgt gaggttataa agcccagtcg
     3481 ccttcgccac gtctttgacc gggacgctcg cgaaggtggc catgctcatg ggcaagaaca
     3541 tcgccaccgt gccgaagccg cgaatgatga gaggccagaa tagctcgctc tcgttggttt
     3601 gagggttgag ctgggccagc atcatcaggg cggtgatcac gatgatggac ccggccgcga
     3661 ccagtactcg cgggtcgaat cgtcgcagca gtcgcgccat gaccggcatc gcgaacgccg
     3721 aagcgagcgc gccggggagc atcagcatgc ccgtttgctg cgacgtgtac cgcatcaccg
     3781 tttgcgcgaa gatgggcacc gcgaacatgg cgccgtacag gcccatcccg atcaccgcgg
     3841 agacgatgca cccggcccag agcgaacggt accgaagcac gcgtaaatcg acgacagggc
     3901 tttcggtcgt cagctgacgg tgcacgaagg cgaccaagca aatgacggcg agcgccgcgc
     3961 agatgcagat gaaggtcgag tcgaaccaac tgtccgaata gccctcttcc aaaaaggtct
     4021 gcagcgcgcc gagccccgcg gcgaggaacc cgagtgaaac ccaatcgacc gagccgctcg
     4081 actgggtcgg gcgatcgtcc ggcaagaagg tcgcggccat gaagacggcg agcactccga
     4141 tcggcaaatt gatgaagaag atccatcgcc aatcgaggtt cgtgacgatg tagccgccga
     4201 gcgtcgggcc aatcgcgggg cccgcaatcg cgaccacacc gaacagcgcc tgagcgctgc
     4261 ctcgctcttc gggcggaaac gtttcccaca gaaacgactg cgccttcgag agcaggccgc
     4321 ctccgaagag gccttgcaac acgcgcgaca gcaccagcat cgggagcgag tgcgagaggc
     4381 cgcacaaaat cgacgcgccc acgaacgcga tcagcgaaaa tacgaagaac cgcttcttgc
     4441 cgaagcgatc gccgagccac gcggagagcg gcaaaatgat gacgttcgcg atcgcgtagg
     4501 aggtgaccac ccagccgact tcgctgagcg tcactccgag cgacgcctgc atctgcggga
     4561 gcgctacgtt gacgatggag gtgtcgacga tttcgagcag cgctccgagc gcgacgctga
     4621 ccgcgatgag ccagcgccgc gtcttgtcgg gcacggcgag cgcgtcgaga gaggcggtgg
     4681 acgtggtcat tcgcgctcgc tcacaaccga gtgttcacgc tgagctcgac ggagagaccc
     4741 gggagcaaca tcatgtcgcc gggcgcgtcg tcgagcttca cccgaaccgg aacgcgctgc
     4801 acgaccttgg taaagttacc ggtggcgttg tcgggcggca acagggcgaa gcgcgcgccg
     4861 gtcgcccccg aaaagctttc gacgtggccg tgcagttcgc gaccataagc gtcgatagtg
     4921 agcgtcgctg gttgacccac gtgcatgtgc gcgagctggg tctctttgaa gttccccgtc
     4981 acccaaaccg gaccgccagc gcgcgcgtcc ggcacgacca tgaccacgct ttgcccgggc
     5041 gaaacgagtt ggcccaccgc gacgctgcgc ttcgagacga tgccgtcgcg cggagcaaaa
     5101 atcttcgtgt aggacaaatc gaggcgcgcc aagtcacgca tcgcctccgc tgtcgcaaca
     5161 cgcgcccgcg ccgaggtggc cttcgcacgc gcttccgcga tctgcgcggt ttgggtgtcg
     5221 ctggcttgcc cgagtcgagc cgaagcttcg gttacacgag cgcgggcttg ggttgcggag
     5281 ctctgcaaag tcacgatgcg cgcctgtgct tgatccatcg cggactgggc cgtgtcgaag
     5341 cttgcctgcg cgctgtcgag ctcggctcgc gagatcgcgt tcatctgtgc caggcgctgc
     5401 gtgcgctcga gatcgagctt tgccttttcg cgcagcgtag tcgccgacgc caggttcgct
     5461 ttggcctcgg cgatctgatc ggcgctcgcc gtcatcgagg cggccgcgcc cgagagcgaa
     5521 gcgcgcgcga tgttttggcc ggcgctcacg ttcttctcgg tgagctcggc ggtggcttcg
     5581 gcagcgtcgg cggatgcctt ggccgcgagg agttcggcct cggcctcggc aaacttcgcc
     5641 ttcgcctgag cgtcatcgag ctcgatcaga agttcaccag ccttgacccg ttgattgtcg
     5701 gcgaagtgaa gcgccgtgac cgtgccgccg actcgagccg gcaccgcgac gacgtccgcg
     5761 tcgagctgag cgtcgtcagt cgattcgagc ccccgatgct gcaaccagta gcctgcgccg
     5821 ccgaccacca gggctgcgag aagccccagc atgatcatgc ggccctttcc cgcaggcttc
     5881 gcaggggcct cggcaggcgc gggagccgcg ggcccaacgg ctgcggaaga agcttgcgaa
     5941 cgaggaggca cggagtcggc ggttgccggg tgcttggcta cggattgagc ggacatggtg
     6001 atcgctgaag agctttctgt cggacgttaa cgcaactatc agttgcttta gataacaccc
     6061 ggcgctataa aagcaacaca tcgttgcgtt aaggcgtatg aagtcttcgt gaagaaccaa
     6121 gaagccgaac aatctcagag ccttagctca gcagcagggc cagccgcgcg ccgcacgggt
     6181 gggcgcagcg cgcgcgtcgt tcgcgatgtg ctcgcggcgg cgctcgaggt ttttgccgag
     6241 cagggctacg ccggtttctc catcgacgac gtcgcgacgc gcgcgtccgt gaacaagacc
     6301 accgtctatc gccgttggcc gaccaaggca gatctggtcg gcgccgctct catcagcctg
     6361 cgcgatcaag accccgagcc gccggacacc ggctccttgc gcgccgatct cgtggcgctg
     6421 cttcatcagc gtgcgactca aatgggcacc ccggcgcgaa gggcgatcat gcaggcgctg
     6481 ttggtgagca actccgagcc ggagctcctg gcgttgactc agaagctgcg gcgtgagcgg
     6541 cccggcatcc cgagggtgct gtttcaacgc gccgtcgagc gaggcgagtt gcctgcagac
     6601 gtggatgtag cgctcatgac cgaggcgttg ttcggggccg tgctgtcgcg cacctggaag
     6661 cgcgagcgcg tttcgcactc cttcctcacg cggctagtcg atctcgtcgt cgcgggcgcg
     6721 gccgccggtg cgggccgtct tcggagctga gcgctccccg cgccctcggt gcccaaccct
     6781 gcccgaactg cctgcgcacg cctcgggagc gccggcaaac tcgccgcatt gagctcggag
     6841 ctgaggcgtt ttgccgcgct tttcccagga gcgaacacac tggatttgcg gttttcacgc
     6901 ttctgtcggg ttcggtctct gttttgcctt agggcgcttg tctcgtgcct tggtgcacgg
     6961 ggccatgcac ggaaacccgg aggagcgaat gaagacgacc gcccacgctt tggctttctc
     7021 ttttggtctc gcctgcgcgg cgagcgctat cagctgtccc gctcaagccg aggagctcac
     7081 cgggccgccg ttggatacca agggtaagcc ggtaaaccca ccggcgccgg tcgag
//
LOCUS       DASTKS010000242         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_707232, whole genome shotgun sequence.
ACCESSION   DASTKS010000242 DASTKS010000000
VERSION     DASTKS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707232"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VFQ35_05060"
     CDS             <1..1237
                     /locus_tag="VFQ35_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9930028.1"
                     /translation="ARAQAGDVRAFESLVEATQERSYALARRILRDDAGARDALQEAY
                     LTAFRRLAELDDAGAFIAWLRRIVTTTALNARRGARTTWLPLDEESAPPVLDAEESRW
                     SAAQQRGLSRALLTLSEDERRLCELHYHGGWTAERLARHAGVPPTTVRKRLQRLRDKL
                     RKEIEMEERSLLGGAAPPADLPVRIAELLARPRLVDLPENPVGAVLAELRAVFHEYRE
                     VGLPEQIDLEAAARQLGGDAVYIDRAKLQRIEGERVLRYDLTLPLLLNVRWSGQPSRL
                     LAAGKVYRQELESATHLEAFHQAELFALDVRGAFDVWTFAGRILAAVDQLFPRAEVRV
                     TPTEYPMCRRAWSLDVLREGDWVEVLAWGEYADWVLTGIGADPVRHTGFGAGIGLERF
                     AGLKYRIDDVRKLATAAIS"
     gene            complement(1657..>3123)
                     /gene="gnd"
                     /locus_tag="VFQ35_05065"
     CDS             complement(1657..>3123)
                     /gene="gnd"
                     /locus_tag="VFQ35_05065"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HET9930029.1"
                     /translation="EDAQMSEPRADIALIGLAVMGQNLVMNMNDHGFTVSVYNRTASV
                     TEEFLSTTARGSKVIGHTDLPALVASLKKPRRVFILVKAGAAVDAVIDQLTPLLEPGD
                     IVIDGGNSHFSDSERRFAALAKKGLRFIGTGVSGGEEGARRGPSIMPGGDPEAWPHVQ
                     QILQSVAARVDDAPCCDWVGKGGAGHYVKMVHNGIEYGDMQLIAEAYQLLHEGLGLGA
                     DELKAVFDQWNRGVLDSYLIEITRDVFGVRDDDGKPLVDKILDAAGQKGTGKWTSNSA
                     LDLGMPVTLIGEAVFARALSSLKDERTRASQVLPGPTGTFSGDKKAFVADVERALYAS
                     KIVSYAQGYMLLREASKEYGWDLNFGSIALMWRGGCIIRSRFLGDIKKAFDTNPKLEN
                     LLVDEFFQRALSESQAGWRRTLSQAITLGIPTPAMSSALAFYDGYRRERLPANLIQAQ
                     RDYFGAHTYERTDKARGEFFHTNWTGRGGNVSSRSYNA"
BASE COUNT          511 a          987 c         1069 g          556 t
ORIGIN      
        1 tgcgcgcgcg caggcgggcg acgttcgcgc gttcgagtcg ctcgtcgaag cgactcaaga
       61 gaggagctac gcgctggcgc ggcggatcct gcgagacgac gcaggggcgc gcgacgcgct
      121 ccaagaggcc tatctcacgg catttcgtcg cctcgccgag ttggacgatg caggggcctt
      181 catcgcttgg ttgcgccgca tcgtgacgac cacggcgctc aacgcgcgcc gcggtgcgcg
      241 cactacttgg ctgccgctcg acgaagagtc cgcgccgccg gtgctcgatg ccgaagaatc
      301 gcgctggagc gcggcccaac agcgcggact ttcgcgcgcg ttgctcacgc tgagcgagga
      361 cgagcggcgg ttgtgcgagc tccattacca cggcggttgg accgcagagc ggctcgctcg
      421 gcacgcaggc gtccctccca cgacggtacg caaaagactc caacgccttc gcgacaaact
      481 gagaaaggag atcgaaatgg aagaacgaag tttgctcggc ggcgcggctc cgcccgcgga
      541 tctgcccgtc cgaattgcag agctcttggc gcgtccgcgg ctcgtcgacc ttcccgagaa
      601 cccagtcggc gcggtcctcg cggagcttcg cgcagttttt cacgagtacc gcgaagtcgg
      661 cttgcccgag cagatcgacc tcgaggcggc tgcgcgacaa ctcggtggag acgccgtcta
      721 catcgaccgc gcgaagctgc agcgcatcga aggcgaacga gtgctgcgct acgatctcac
      781 gctgccgctc ttgctgaacg tgcgttggtc ggggcagccg tcacgcctgc tcgcggccgg
      841 aaaagtctat cgccaggagc tcgagagcgc gacacacctg gaagcgtttc atcaggcgga
      901 gttgttcgcg ctcgatgttc gcggcgcgtt cgatgtgtgg acgttcgcgg ggcgcatcct
      961 ggccgcggta gatcagctgt ttccccgcgc cgaagtgcgc gtgacgccga cggagtaccc
     1021 gatgtgcagg cgggcttgga gcctcgatgt gctgcgcgaa ggcgattggg tcgaggtgct
     1081 ggcgtggggg gagtacgcgg actgggttct gaccggtatt ggcgccgatc cggttcggca
     1141 caccgggttc ggtgcgggca tcgggctcga acggttcgca gggctcaaat accgaatcga
     1201 cgacgttcgc aagcttgcga ccgccgcgat cagttagccg agcgatccgt tgtcggaagt
     1261 gactaacgcg gctggcggcg gtgaggtgag ggcgcacgcg gagcccccct cccacggccg
     1321 cccgcggcga agcgaggacg gacgtttggg aggggcggcg acacgcgcag cttgctcgtc
     1381 gcggcgctcg tcattcaaca acacgctgtt ggctcttctt cggcgcgtgc cgtggcgtct
     1441 gcagatctcc gaacacgcat gggcctgccg ttttcgggtt ccggggcccc gaaaaccaca
     1501 caaaccacag cgtcggaacg aactctttcg aagcgcgagc gacaagccac tctttcgtgc
     1561 cgagccctct gcgagcccac gaaacgaagg cttttctgct cgcgacatgc gacgaacccg
     1621 gaatgtggca ggcaggttct tcgaacttcg acccgctcag gcgttgtagg agcgcgagct
     1681 cacgttgccg ccgcgcccgg tccagttcgt gtggaagaac tcaccgcgcg ctttgtcggt
     1741 gcgctcgtag gtgtgcgctc cgaagtagtc gcgctgcgcc tggatcaggt tggccgggag
     1801 ccgttcgcgc cggtagccgt cgtagaaggc gagggcagag ctcatggcgg gcgtggggat
     1861 gccgagcgtg atcgcttgcg acagcgtgcg acgccaaccc gcttgcgact cgctgagcgc
     1921 gcgttggaag aactcgtcga cgagtaggtt ctcgagcttc gggttggtat cgaaggcctt
     1981 cttgatgtcg cccaagaatc ggcttcggat gatgcagccg ccgcgccaca tgagcgcgat
     2041 ggagccgaag ttgagatccc agccgtactc cttgctcgct tcgcggagca gcatgtaccc
     2101 ctgggcgtag ctgacgatct tggaggcgta gagcgcccgt tcgacgtcgg caacgaaggc
     2161 cttcttgtca ccgctgaagg tgccggttgg tccgggcagc acctgcgagg cgcgcgtacg
     2221 ctcgtctttg agcgacgaca gcgcgcgcgc gaacacggcc tcgccgatga gcgtcactgg
     2281 catcccgagg tcgagcgccg aattgctcgt ccacttgcca gtgcccttct gaccggcggc
     2341 gtccaagatc ttgtcgacga gtggcttgcc gtcgtcgtcg cgcaccccga atacgtcgcg
     2401 cgtgatctcg atgaggtagc tgtcgagcac gccgcggttc cactggtcga agacggcctt
     2461 gagctcgtcg gcgccgagac cgagcccttc gtggaggagc tgatacgcct ccgcgatgag
     2521 ctgcatgtcg ccgtactcga tgccgttgtg caccatcttc acgtagtgac cggcgccgcc
     2581 cttgccgacc caatcgcaac aaggtgcgtc gtcgacgcga gccgccacac tctgcaagat
     2641 ctgttgtacg tgcggccacg cctccggatc gccgccgggc atgatcgacg ggccgcggcg
     2701 cgcgccttct tcgcccccgg agacgccggt gccgatgaag cgcaacccct tcttggcgag
     2761 ggcagcgaag cgccgctcgc tatcgctgaa gtgcgagttg ccgccgtcga tgacgatgtc
     2821 tcccggctcg agcaggggag tgagctggtc gatgacggcg tcgacggctg ctccggcttt
     2881 gacgaggatg aagacgcggc gtggcttctt cagcgaagcg acgagcgcgg gcagatccgt
     2941 gtgaccgatg accttcgagc cgcgcgcggt cgtggacaag aactcctcgg tgacgctcgc
     3001 ggtgcggttg taaaccgaga ccgtgaagcc gtggtcgttc atgttcatga cgaggttttg
     3061 acccatcacg gccagcccga tgagcgcgat atctgcgcgg ggttccgaca tctgtgcgtc
     3121 ctc
//
LOCUS       DASTKS010000243         6685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_39503,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000243 DASTKS010000000
VERSION     DASTKS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39503"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            636..3356
                     /locus_tag="VFQ35_05070"
     CDS             636..3356
                     /locus_tag="VFQ35_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9930030.1"
                     /translation="MHDDVFEQIFEGHPDAWSVVELDRDQIFVVRCNQAQARRFGMTP
                     SELQMTDLRQLVAPEHYAGWLSMLESVAVAGEAQTFEYQGPPLVGGLVRATMMPLRAG
                     ASGLARFFVHIQDETSRQLAQRALVRSEARLAQAQRIGKTGDWELDLVTGTGTWSREM
                     FRIMGWHGAEPPTLEQFEALAHLDDRKSLARFHRDVLEADSPQEFVFRLVTPSGDTRR
                     IAVSAVCTRDPSGKPVTITGVNRDITGLEESLESARRHGDALEVLFEENVNGVYFSLL
                     DEPVDWDNAADKDVLADWALEHARPVRVNRALCAIFGAEAHELIGRPLSNFFPDRLEG
                     RKQFRALYDAGRLHLSRTSRLRADGTRWLADLDLVVTRDQRGRITGQFGTLRDVTEQE
                     RAAEALRRSEARLALALESAELYVWTYDLRTQRIESDPRWPARFGYTIEEFADREFAR
                     SLVNPTDQAATDRAFADLLAGNVPSIAVTYRLATRSEGFRWIEIFGKISERDASGAPL
                     QISGICRDVTEQRTLEQRLLAAERMASLGTLAAGIGHEINNPLTYLSANLALMDDALD
                     GFAKQAVTSTNVDELRRMLRDARDGVMRIVRIVKDLRSLSRGSAVVRQRVDLKTVLER
                     CLELAEHEIRHRANVQRNFEALPEIVADEGRLIQLFLNLLVNAAQAINEGAADSNFIR
                     VSSKVSSSGGVVVEVEDTGKGMEPDVLSRIFDPFFTTKNVGEGIGLGLPICHSIVRDL
                     GGSIEAESVPGRGSLFRVRLPRGELAEEPVPAPAPPEPPPAPKSKRLRILVIDDEATV
                     GRLLTRILSGHEVVVETRAGDALVRLKNGEGFDRILCDVMMPETSGVDFYELVKKLRP
                     ELLSRILFMTGGAFSARAAAFLAAIPNVTLEKPFDPVLLRATVEKPPPGS"
     gene            complement(3362..3571)
                     /locus_tag="VFQ35_05075"
     CDS             complement(3362..3571)
                     /locus_tag="VFQ35_05075"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET9930031.1"
                     /translation="MDRLSVQSTSVVSVGYDPEAHFLEVEYHGGRVYRYDSVPHAAYR
                     LLLKAPSVGQYINAVIKPRFRATRL"
     gene            complement(3672..4394)
                     /locus_tag="VFQ35_05080"
     CDS             complement(3672..4394)
                     /locus_tag="VFQ35_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930032.1"
                     /translation="MTPSARARLFVIALLGGACTYDFQDFVSNESTGGGTAEAGSSGG
                     GARGGTSGSSTSGGSGSSEGGSSAQGGSASAGASSHAGAGSGGASSATGGKSGSAGAA
                     SGGRTSSGGATSSTGGANSSGGGGNKACAGTTLNSICWYLGAANQSCSDVCTSHGGVN
                     AAAEKMVGVPAQGGSVTSCALVLAMLGTRDVPAEATRSDGNGLGCHVLGGRSNTAYWL
                     SSPRFSATAHLSNASIACGCNQ"
     gene            complement(4394..5365)
                     /locus_tag="VFQ35_05085"
     CDS             complement(4394..5365)
                     /locus_tag="VFQ35_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930033.1"
                     /translation="MNLRLGLPWILLACGALLAPTPARGDDATPPDANDSAKVFADAV
                     KTFKAGRAKDALPSFERVAAATKSPNAELYVGYCLAELGRHREAHKAFSLTIRYALAL
                     KDPRYEAARESAQDQVLGLNLRLARLVLSFVDAPAGLNVLVDEQPVPADELGSPIVLD
                     PGAHHIRATADGAQPIERDETIEAGGSKTLTLSFAKNTANPEKTVVVLPSRSSESTLR
                     MLGFVTGGVAVAGLSVFAITGLKARSIHSQLESECARGCSDASHLDMVGRGKSMQTVA
                     NVSLAIGAVSAAASATLLYFGYRQTSTPEAAVAVEPGGLSLHYRGTF"
     gene            complement(5366..>6685)
                     /locus_tag="VFQ35_05090"
     CDS             complement(5366..>6685)
                     /locus_tag="VFQ35_05090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930034.1"
                     /translation="IARLMGREGFSRVVAAKRMPRHYLADPEFKRMFLEEALLAARVR
                     HPNVVPIVDVLSHESELIIVMEYVHGESLLALMRLAHSSGGFPVPIAASIAVGALQGL
                     HAAHEAKDERGEPLGIVHRDVSPHNVLVSTDGVARVVDFGVAKAVRAKEDTKPGVLKG
                     KFSYMAPEVVRGAPTTRQADVFSAAVVIWEMLAGRKLFAGASEQERLIAIVGGKYPSP
                     RQFNSAVSPALERIVMKGLMPDPAARYASALEMAIDLEREVSLASQRVVGEWVSSRAA
                     DELDRRAMLLHDIETSAVIPAAAVHDRHSMFGVGHSVTAPSAVISALPPTEPRRRPFP
                     LIVSAAALVAFGIIFAFRGGHRTTEVTLPSANPGAVASPPTSAPAPAPSAREAQAPAA
                     PPPPTAPAPVESATTKASTPQPPRPTQTPKPAARPKSRGKHFLPSEL"
BASE COUNT         1082 a         2184 c         2256 g         1163 t
ORIGIN      
        1 ccgtgaaacg agaatgctcg cagtaagtat cactctaaag tgaggaactc ggggcgtgcc
       61 gaattatccg ccctgctgcg agccaaggcc cgtcccgctg ccgcggcgcc ggctcggtca
      121 ctccgctccg cgagggcttc aaggcttcct tggtcgatcg gttccccgga acgcgccggt
      181 ctctcttgct tcctcgctct cgcgacctgg cagaagcttg cagcgaacgc gggtcagtgt
      241 cgacgcgcgg ccgcaaacga gcgtgttcgt gcgcggaagt cggagcaaca tatcgtttaa
      301 cgatgtgtgt ggacgcttgc gcgcgccacc gggagcgtgc ctcgagcggg tcgacaggat
      361 gctgccaaga ccggaactct cgagtcgtgg cgatattcgc cggcgcggcc cgtgctcggc
      421 cctgcgccac acggagccgt gagatttctc gcttcgcgat gcaacaaagt acggcgccgc
      481 gcggagcccg agtgagcgcg ctccgtcgaa agtggcgagc gcaaagtgtc acgccgctgc
      541 cgctctccgc gccatcggct tcgccgcttg ccgactcgaa gcctcccgcc taatctcgga
      601 atttccgcga cccgggtttc gagagacgaa ctcacgtgca cgacgacgtg ttcgagcaga
      661 ttttcgaagg gcaccctgac gcgtggtccg tggtcgagct cgatcgcgac cagatcttcg
      721 tagtccgctg caatcaggct caggctcgtc gtttcggcat gaccccgagc gaattgcaaa
      781 tgacggatct ccgtcagctg gtcgcgccgg agcattacgc gggatggctc tccatgctcg
      841 agagcgtcgc cgttgccgga gaagcgcaga ctttcgaata ccaggggccg ccgctcgtcg
      901 gcggcttggt gcgcgccacc atgatgccct tgcgcgcggg cgcgagcggg ttggcacgat
      961 ttttcgtgca tatccaagac gagacttctc ggcaattggc gcagcgagcg ctcgttcgca
     1021 gcgaggctcg cctcgcgcag gcgcaaagga taggcaagac gggcgactgg gagctcgacc
     1081 tcgtgacggg gacgggaaca tggtcgcgag agatgttccg aatcatgggc tggcacggcg
     1141 cggagccgcc caccctcgag caattcgaag ctttggcgca cctcgatgat cgcaagtcgt
     1201 tggcgcgttt tcatcgcgac gtgctggagg cggattcccc gcaagagttc gtgttccgac
     1261 tggtgacgcc gtccggcgac acacgccgga tcgccgtgag cgcggtgtgc accagggacc
     1321 cgtccgggaa gccggtcacg atcacgggtg tcaatcgcga catcacgggg ctcgaagaat
     1381 cgctcgagtc ggcgcggcgg cacggcgatg cgctcgaagt tctgttcgag gagaacgtca
     1441 acggcgtcta cttctcactg ctcgacgagc cagtcgattg ggacaacgcg gccgacaagg
     1501 acgtgctcgc cgattgggcc ctcgagcatg ctcgcccggt gcgggtgaat cgggctctct
     1561 gcgcgatttt cggggctgaa gcccacgagc tcatcgggcg accgctctcc aatttcttcc
     1621 cggatagact ggagggacgc aaacagtttc gagccttgta cgacgcgggt cggctgcatt
     1681 tgtcgcgcac ttcgcgactg cgcgccgacg gcactcgttg gctggccgat ctcgacttgg
     1741 tcgtcacgcg cgaccagcgg ggtcgcatca ccggtcagtt cggaacgctg cgcgacgtca
     1801 ccgagcagga gcgggccgcc gaggcgctca ggcgcagcga agctaggctt gcgctcgctc
     1861 tggagagcgc cgagctctac gtctggacgt atgacttgcg aactcagcgc atcgaatcgg
     1921 atccgcgctg gcccgcgcgt ttcggttaca ccatcgagga gttcgccgat cgagagtttg
     1981 cgcgtagcct cgtcaacccc accgaccagg ccgcgaccga ccgagccttc gcggaccttc
     2041 tcgctggcaa cgtgccgagc atcgcggtga cgtaccggct cgcgacgcgt tcggaggggt
     2101 tccgctggat cgagatcttc gggaagattt cggagcgcga cgcgagcggg gcgcccctcc
     2161 aaatctccgg aatctgtcgt gacgttaccg agcaacgcac gctcgaacag cgcctgttgg
     2221 cagccgagcg catggcttca ctgggcactc tcgccgcggg tatcggccac gagatcaata
     2281 accctctcac gtatttgagc gcgaacctcg ctctgatgga cgacgccttg gacggtttcg
     2341 ccaagcaggc ggtaacctcg acgaacgtcg acgagctccg acgcatgctg cgtgacgcgc
     2401 gagacggggt gatgcgcatc gtccgtatcg tcaaagatct gcgctcgctc agccggggca
     2461 gcgccgtcgt tcggcagcga gtcgacctga agaccgtgct cgagcgctgt ctcgaactcg
     2521 cggagcacga gattcgtcac cgcgcgaacg ttcaacgcaa cttcgaggct ttgcccgaga
     2581 tcgtcgccga cgagggccgg ctgattcagc tctttttgaa cctgctcgtg aacgcggccc
     2641 aagcgatcaa cgaaggtgcg gcagattcga acttcattcg ggtaagctcg aaggtgagct
     2701 cgagtggtgg ggtcgtcgtc gaagtggagg acacgggcaa ggggatggag cccgatgttc
     2761 tttcgcgcat cttcgatccg ttcttcacga ccaagaacgt cggggaaggc attgggctcg
     2821 ggttaccgat ctgccattcg attgtcaggg atcttggcgg cagcatcgag gccgagagcg
     2881 tccccggacg cggctcgctg ttccgagtgc gcctcccgcg cggcgagctc gctgaagagc
     2941 ccgtcccagc gcccgcgccg ccagaacctc cgcctgcgcc gaagtcgaag cgcctgcgta
     3001 tcctcgtcat cgacgacgag gcaacggttg ggcgacttct cactcgaatt ctgagcggcc
     3061 atgaggtcgt ggtcgagacc cgcgccggcg acgcgctcgt ccggctgaag aacggtgaag
     3121 ggttcgaccg aatcctctgc gacgtgatga tgccggagac cagcggcgtg gacttctatg
     3181 aactcgtgaa gaagcttcgc cccgagctgc tgtcgcgcat cctgttcatg actggcgggg
     3241 ccttcagcgc gagggcggcc gccttcctcg ccgcgatacc caacgtcact ttggaaaagc
     3301 cgttcgaccc ggtgctgctg cgcgcgacgg tcgagaagcc gccaccaggc tcttgaggcg
     3361 cttacagccg tgtcgcgcga aagcgtggct tgatcacggc gttgatgtac tggccaaccg
     3421 agggcgcttt gagcaacaag cgatatgcgg cgtggggcac gctgtcatag cgatatacgc
     3481 gcccgccgtg gtactcgact tccaaaaagt gagcctcggg atcgtaccct accgagacga
     3541 cgctggtcga ttgcacgctg agacgatcca tcgcttcccg ggagagtcga cgcccggggc
     3601 gaacttgtca acggaaagcc aatccgcgac gaggtcgcga gaagtggtcc gcgcttcacg
     3661 ggcggaccgt ttcactgatt gcagccgcag gcgatgcttg cgttcgacaa gtgagcggtc
     3721 gcgctgaagc gcggtgagct cagccaatac gcagtattcg agcgcccgcc gagcacgtgg
     3781 cagccgagcc cgttgccgtc gctgcgcgtg gcttcggcgg ggacgtcgcg agtgccgagc
     3841 atggcaagaa ccagcgcgca gctcgtgacg ctaccgcctt gtgctggcac ccccaccatc
     3901 ttctcggcgg cggcgtttac cccaccgtgc gacgtgcaga cgtcactgca gctttggttc
     3961 gccgcgccca ggtaccagca gatgctgttc aaggtcgtgc ccgcgcaggc tttgttgcct
     4021 ccgcccccgc tcgagttggc gccgcccgta gaactcgtag cgccgccgct cgacgttcga
     4081 ccgccggagg ccgcgcctgc gcttccgctc ttgccgccgg ttgcgctcga ggcgccgccg
     4141 ctccctgcgc ctgcatggga cgacgcgccc gcgctcgcgc ttccgccctg ggccgagcta
     4201 ccgccctcgc tgcttcccga accgccgctc gtcgagctgc cgctcgtccc tccgcgcgct
     4261 ccgccgccgc tcgacccagc ctccgcggtg ccgccgcccg tggactcgtt gctcacgaaa
     4321 tcctggaagt cgtaggtgca ggcgccgccg aggagcgcga tgacgaagag ccgtgcgcgt
     4381 gcgctcggag tcatcaaaac gttcctcggt agtgcagcga gagcccgccg ggctcgacgg
     4441 ccaccgccgc ctccggagtg ctggtctgcc gatacccgaa gtagagcagc gtggcgctcg
     4501 cggcagcgct caccgcgccg atcgcgaggc tcacgttggc gaccgtctgc atggacttgc
     4561 cgcggccgac catgtcgagg tgactcgcgt cgctgcagcc tcgagcgcac tcgctttcga
     4621 gctgcgaatg gatgctgcgc gccttgagac cggtgatcgc gaacaccgaa agccccgcta
     4681 ccgcgacgcc gcccgtcacg aagccgagca tacgaagcgt gctttcgctg ctacgcgagg
     4741 gcagcacgac cacggtcttc tccgggtttg cggtgttctt cgcgaagctc agggtgaggg
     4801 tcttgctgcc gccggcctcg atcgtttcgt cgcgctcgat aggttgcgct ccgtccgccg
     4861 tcgcccggat gtggtgcgcg cccgggtcca gcacgatggg cgagccgagc tcatcggcag
     4921 ggacgggttg ctcgtcgacg agcacgttca ggccagcggg ggcgtcgacg aacgagagga
     4981 cgagcctcgc gaggcgcagg ttcaagccga gcacttggtc ttgcgccgac tcacgagctg
     5041 cctcgtagcg tggatctttc aacgcgagcg cgtagcgaat ggtgagagag aacgccttgt
     5101 gggcttcccg atggcggccg agctcggcca agcaataacc gacgtagagc tcggcgttgg
     5161 ggctcttggt ggcggcggcg acgcgctcga agctcggtag agcgtccttc gccctgccgg
     5221 ctttgaatgt ctttacggcg tcggcgaaga ccttcgcgct gtcgttggcg tcgggcggcg
     5281 tcgcgtcgtc gccgcgggcg ggcgtcggag cgagcagcgc gccgcacgcg aggaggatcc
     5341 agggcaagcc gaggcgtagg ttcaactaaa gctcgctggg gagaaaatgc ttgccgcggc
     5401 tcttcggtcg cgcggcgggc ttgggagttt gcgttggacg tggcggttgg ggagtcgacg
     5461 ctttggtcgt tgcgctctcg acgggagccg gggccgtggg cggtggcggc gcggcgggcg
     5521 cctgagcctc gcgcgcgctc ggggctgggg ctggtgcgct cgttggcggg ctcgcaaccg
     5581 cgcccggatt ggcgctcggc agcgtgacct ccgtcgttcg atgtccgcct cggaaagcga
     5641 agatgatgcc gaacgcgacg agagcggccg ccgacacgat gagcgggaac ggacgtcggc
     5701 gcggctcggt cggtggtagc gccgaaatca ccgcgctcgg tgccgtcacc gaatggccca
     5761 cgccgaacat gctgtgccga tcgtgaaccg ctgccgccgg tatcaccgca gacgtttcga
     5821 tgtcgtgcag gagcatcgct cggcgatcga gctcgtccgc ggctcgagaa gagacccact
     5881 cgccgacgac gcgctgggaa gcgaggctca cttcgcgctc gaggtcgatc gccatttcga
     5941 gcgcgctcgc atagcgcgcc gccggatccg gcatcaggcc tttcatcacg atgcgctcga
     6001 gcgcagggct cacggctgaa ttgaattggc gcggcgaggg gtactttcca cccactatcg
     6061 cgatcaagcg ttcctgctcg ctcgcgccgg cgaagagctt gcggcccgcg agcatctccc
     6121 aaatcacgac cgccgccgag aacacgtcgg cctgtcgcgt cgtcggcgca ccgcgcacga
     6181 cctcgggcgc catgtacgag aacttcccct tcagcacgcc gggcttcgtg tcttctttcg
     6241 cgcgcaccgc cttggccacg ccgaagtcca cgacgcgcgc gacgccgtcg gtgctgacga
     6301 gaacgttgtg cggcgacacg tcgcggtgca cgatgccgag cggctctccg cgctcgtcct
     6361 tggcctcgtg cgcggcgtgc aagccctgca gcgcgccgac cgcgatcgac gcggcgatcg
     6421 ggaccgggaa gccgcccgac gagtgggcga ggcgcatcag cgcgagcagc gactcgccgt
     6481 gcacgtactc catcacgatg atgagctcgc tctcgtgaga cagcacgtcc acgatcggca
     6541 cgacgttggg gtggcgcacg cgcgcggcga gcagcgcctc ttcgaggaac atgcgcttga
     6601 actcggggtc tgccaggtag tggcgaggca tgcgcttcgc cgcgacgacg cgggagaagc
     6661 cttcgcgccc catcagtcgc gcgat
//
LOCUS       DASTKS010000244         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_486045, whole genome shotgun sequence.
ACCESSION   DASTKS010000244 DASTKS010000000
VERSION     DASTKS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486045"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VFQ35_05095"
     CDS             <1..1344
                     /locus_tag="VFQ35_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9930035.1"
                     /translation="EPSILSTLKKAISLEGYNVDVAGGIGIAEERIARKNYDVLLLDV
                     ALPDGDGLEFLSRVRAANNDAAVIMMSGHATIDAAVRATKLGALDFLEKPLSTDRLLV
                     MLENALRLVRAEAEARELRSAAGYFDELLGDSRVMRDLREKVLRAARSNASVLITGER
                     GTGKELVSRAIHRASPRASAPLERLNCAAVPAELIESELFGHEAGAFTGATKQRRGKF
                     ERASGGTLFLDEVGDMPLAMQAKLLRVLQESEIERVGGNETLRIDVRVVAATNKDLRA
                     ATANGSFRADLFDRLNVVPLELPPLRARREDIPSLADAFLDSARSSNGRSGVRLTDDA
                     RAALTRLPFPGNVRELKNLVERLVILNSDDVVTAADVERFSGMGATPSVNGLYRPGVP
                     FRVLSEEAERVIIEEALAQNGGQMAATARALGLERSHLYKKAKALGLRGDGSDDD"
     gene            complement(1409..>3399)
                     /locus_tag="VFQ35_05100"
     CDS             complement(1409..>3399)
                     /locus_tag="VFQ35_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930036.1"
                     /translation="ARLPDPHFVLAGIALRDMELESADRELDAGLRAAPADLKLLSLR
                     AVVRFLADDRDGFARAKRGVLERNPEFSRLFAILGEYADWEHRYDEIVVLMREALTID
                     QDDAGAHAQLGLNLIRAGDEKDGVSELDRAFKLDPYNVRVYNTLGLYETLIPKAYVSV
                     STPRFNLRYHKDDRAVLERYVPELLNRAFETMRVSYDFTPETPIGIELYAERQSFAIR
                     TSGLPQTAIQGVCFGHTLAAMSPQQESFNLGMTLWHELSHVFHIQLSKAHVPRWFTEG
                     LAEYETLVTRPEWSRQHDPELYEMMRVGKLPSVGKMSRAFTRAEALSDVATAYYASTK
                     IVEMLVRDHGRPKIAKMLRLWGEGRRTEQVFQEALGTTPEAIDQAFRTYLGQSLARYD
                     KQFMPIARARPRDVLEAAARSAPSDVSLSLELALARMNSGDLEATAKSLAAVLSRDPK
                     NAQARFMSARLASERDDKQRATRELRALVKDGVDGYAIEVALGDLARAHNDSAGARAA
                     FEQAYRFDPTQAEPLLALADIAASEKVSDDELDKLEKVAPLSAHSAAVHRRLLTALLE
                     KKRFADAVRAGEAALYADINGLETHVLFARALEENGDLKRARFELESALACPGEPPQL
                     ADVHTRLAELLKRLKAPAEARRHEAEAKKLRAVAPKLP"
BASE COUNT          553 a         1149 c         1107 g          590 t
ORIGIN      
        1 gagccctcga tcctctccac actcaagaag gcgatttcgc tcgagggcta caacgtcgac
       61 gtcgccggcg ggatcgggat cgccgaagag cgcattgctc gcaagaacta cgacgtgctc
      121 ttgctcgacg tcgcgctgcc cgatggtgac ggcctcgagt ttctgtcgcg agtccgcgct
      181 gcgaacaacg acgcggccgt gatcatgatg agcgggcacg cgacgatcga cgccgcggtg
      241 cgcgcgacga agctcggcgc gctcgacttc ctcgagaagc ccctctcgac cgatcgcctc
      301 ctcgtgatgc tcgaaaacgc tctccgcctg gtgcgcgccg aggccgaagc gcgcgagctc
      361 cgcagcgcgg ccggctactt cgacgagctg ctcggagaca gccgcgtgat gcgtgatttg
      421 cgcgagaagg tgctgcgcgc cgcccgctcg aacgcgagcg tgctcatcac cggcgagcgc
      481 ggtaccggaa aggagctggt ctcccgggcg atccaccgtg cttcaccgcg cgcctcggcg
      541 ccgctcgagc gactcaactg cgcggccgtt cccgcggagc tcatcgaaag cgagctgttc
      601 ggccacgagg cgggcgcatt cacgggagcc accaaacagc gccgcggaaa gttcgagcgc
      661 gcgagcggag gcaccctgtt tctcgacgag gtcggcgaca tgccgctcgc catgcaggcc
      721 aagctcctcc gggtactgca ggagagcgaa atcgagcggg taggcgggaa cgagacgctt
      781 cgcatcgacg ttcgcgtcgt cgccgcgacg aacaaagatc tgcgggccgc tactgcgaac
      841 ggctcatttc gcgccgattt gttcgatcgc ttgaacgtcg ttccgctcga gctgccgccg
      901 ctgcgcgctc gccgtgaaga catcccgagc ctcgccgacg cattcctcga tagcgctcgc
      961 agcagcaacg ggcggagcgg cgtgcgcctc actgacgacg ctcgcgctgc gctgacgcgc
     1021 ttgccctttc ctggcaatgt gcgcgagctc aaaaatctcg tcgagcgcct cgtgatcttg
     1081 aactccgacg acgtcgtgac cgcggcggac gtcgagcgct tctccggaat gggcgcgacc
     1141 cccagcgtga acggcctcta ccgacccggg gtgccatttc gggtgctctc ggaggaggcc
     1201 gaacgcgtga tcatcgaaga ggctctggct caaaacggcg gccaaatggc ggcgacggcg
     1261 cgggctctgg ggctcgagcg gagccatctg tacaagaagg ccaaggcgct cgggttgcgc
     1321 ggcgacggca gcgacgacga ctaggtctcc ggactttctc ctcgaagcct cgccctcgcg
     1381 gtacacacga gcgcgcccag tccctccttc agggtaactt cggcgcgacc gccctgagtt
     1441 tcttggcttc ggcttcgtgg cgccgcgcct cggccggcgc cttcaatcgc ttcaggagct
     1501 cagccaaccg cgtgtgcacg tctgccaact gagggggctc gccggggcag gcgagcgcac
     1561 tctcgagctc gaagcgcgct cgcttgagat ctccgttctc ctcgagcgcc cgagcgaaca
     1621 agacatgggt ctcgagtccg ttgatgtccg cgtacagggc ggcttctccg gcgcggacgg
     1681 cgtcggcgaa ccgcttcttc tcgagtagcg ccgtcagtag gcgccggtgc accgccgccg
     1741 agtgcgcgga aagcggcgcg accttctcga gcttgtcgag ctcgtcgtcg ctcaccttct
     1801 cgctcgcggc gatgtcggcc aaagccaaaa gaggctcagc ttgcgtcggg tcgaagcgat
     1861 acgcctgctc gaacgccgcg cgcgcgccgg cgctgtcgtt atgcgcccgc gcgagatccc
     1921 cgagcgcgac ttcgatcgcg tagccgtcga ctccatcttt gaccagcgcc cgaagctctc
     1981 gtgtcgcgcg ctgcttgtcg tcccgctccg acgcgagccg cgccgacatg aagcgcgcct
     2041 gcgcgttctt ggggtctcga gacagtacgg ccgcgagcga cttggccgtc gcctccagat
     2101 ccccgctgtt cattcgagcc agcgcgagct cgagagacag cgacacgtcg ctcggcgcgc
     2161 tccgggccgc cgcctccaac acgtcgcgcg gtcgagcccg tgcgatgggc atgaactgct
     2221 tgtcgtagcg agccaatgac tgcccgaggt acgtcctgaa cgcctgatcg atcgcctccg
     2281 gagtcgtccc gagcgcctct tggaatacct gctcggtgcg gcggccttcg ccccaaaggc
     2341 gcaacatctt cgcgattttg gggcgtccgt ggtcgcgcac cagcatttcc acgatcttcg
     2401 tcgaggcata gtacgcggtc gcgacgtcgc tcagcgcttc ggcccgggtg aaagcgcggc
     2461 tcatcttacc gacgctcggc agctttccga cccgcatcat ctcgtagagc tccgggtcgt
     2521 gttggcgcga ccactccggg cgcgtcacga gcgtctcgta ctcggcgagg ccctcggtga
     2581 accagcgcgg cacgtgcgct ttggagagct gaatgtgaaa gacgtgcgag agctcgtgcc
     2641 agagcgtcat gccgaggttg aacgactctt gctgcgggct catcgcggcg agcgtgtgcc
     2701 cgaagcaaac gccttgaatc gccgtctgcg gtaagccgct ggtacggatc gcgaagctct
     2761 gccgctccgc gtagagctcg attccaatcg gagtttccgg cgtgaagtcg taactcacgc
     2821 gcatcgtttc gaacgcgcgg ttcaggagtt ccggcacgta gcgctcgaga accgctcggt
     2881 cgtctttgtg ataacggagg ttgaagcgcg gcgtgctgac gctgacgtac gctttcggga
     2941 tgagcgtctc gtaaaggccg agcgtgttgt aaacgcgcac gttgtacgga tcgagcttga
     3001 aggcacgatc gagctcgctc acgccgtcct tctcgtcgcc ggcgcggatc agattcagac
     3061 cgagctgcgc gtgcgcaccc gcatcgtcct gatcgatggt gagcgcctcg cgcatcaaca
     3121 cgacgatctc gtcgtaacgg tgctcccagt cggcgtactc ccccaagatc gcaaaaagcc
     3181 ggctaaattc gggatttcgc tcgagcacgc cgcgcttcgc gcgcgcgaag ccgtcgcggt
     3241 cgtcggccaa gaagcgcacg actgcgcgca ggctgagcag cttcaagtcg gccggcgcag
     3301 cgcgcagccc cgcgtcgagc tctctgtcgg cgctctcgag ctccatgtcg cgcaaggcga
     3361 tgcccgcgag cacgaaatgc ggatcgggta agcgcgcgt
//
LOCUS       DASTKS010000245         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1154384, whole genome shotgun sequence.
ACCESSION   DASTKS010000245 DASTKS010000000
VERSION     DASTKS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154384"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1246
                     /locus_tag="VFQ35_05105"
     CDS             137..1246
                     /locus_tag="VFQ35_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9930037.1"
                     /translation="MKSMFRDPRPRERARVAIVGGGVSGVTAAFRCAQYGYDTTVFEA
                     DARLGGSLWSEQDQGFLIEHGAESFDAGSEAVRALAEDLRLTSEIVPAATARSYGFDG
                     HSLFVLDPGSAGASGASASSPLGRSVATFGSGMGRLVEGLASALATRGEIHLNTPIKR
                     LEPTTAGWRLHSARGPGGDFDAVVIASDARTASRLLSELFGSPALALRSSRSVPIVSV
                     SLAFAREAIAHPLDATGFITGFGVELDDCVKCSFVSSTFHGRAPDGSVLVRIFLRPNE
                     ADLEAPLELFRERATKVAMRVLEANAAPQRAWEARWREGFAVLDEEQLRRVALLEYTL
                     GRMPIALAGTAFHGDGVDGAVRSADNAARKLRRLC"
     gene            complement(1238..1993)
                     /locus_tag="VFQ35_05110"
     CDS             complement(1238..1993)
                     /locus_tag="VFQ35_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9930038.1"
                     /translation="MTTKPISGKFGGIDRLLAVSHAFSGSDPALLLQLGAQTLRARRV
                     RGTALFEQGTPADALCLVTSGIVKLTRATPDIELNILGLFGPRECVGMLSLAPGATHQ
                     GTAYAASDSVEYLRIPVNVLHEGIARDPRLAAALLRASSNYVDVLTTKVDVLSAGSVS
                     RRLARLFLTLGERFGDELEEGSLVIPVSLSRRELSLLVGSCMESVIRVVSAWQKQGLL
                     RTSPSGFELITPSAFRELAARGDAERDFAAWSA"
     gene            2368..>2889
                     /locus_tag="VFQ35_05115"
     CDS             2368..>2889
                     /locus_tag="VFQ35_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930039.1"
                     /translation="MNDETNVPPSVEPQLNARVGVRHESLEAEPPAEPHSRIWERWAL
                     KLTSPASVLVSVPLSAFVVGSSLVLLSYQRFVSLDERAALDRAELRRDQAELALQRSL
                     ATADPLLDRVRDVARAAATSSIETSALALANLAIQREGLTWLSISLPDGSFRGVFRDK
                     DGSLSFQESRIVDG"
BASE COUNT          513 a          941 c          930 g          505 t
ORIGIN      
        1 tcgcaaccga gccgaatttg gctgtgttcg tcgcacggcg cctggttcaa cgcaaatctt
       61 tcgctcgacg cgccgtcaat gacgaacgtc atgcgcgagg tactcgctca gtgccgccaa
      121 tttatcgaca ggttagatga aatcaatgtt ccgcgaccca cgcccccgcg aaagagcgcg
      181 cgtcgcgatc gtgggtgggg gggtgagcgg cgtgacggcg gcgtttcgct gcgcccaata
      241 cggctacgac accaccgttt tcgaagccga cgctcggctc ggcggcagtc tctggagcga
      301 acaggaccaa ggcttcttga tcgagcacgg cgcagagagc ttcgacgccg gaagcgaggc
      361 cgtgcgagcg ctcgccgaag acttgcgcct caccagcgag attgtcccgg cggccactgc
      421 gcgctcgtat gggttcgacg ggcactccct gttcgtgctc gatcccggca gcgcgggcgc
      481 gtcgggcgcg tctgcatcga gcccgctcgg aaggagcgtc gcgacttttg gttcagggat
      541 gggtcgactc gtcgagggct tggccagcgc gctcgcgacc cgcggtgaga ttcacttgaa
      601 cacgccgatc aaacgcctcg agcctacgac cgcgggctgg cggttgcatt cggcgcgcgg
      661 ccccggcggt gacttcgatg ccgtcgtcat cgcgagcgat gcgcgcacgg cgagccggct
      721 gctctcggag ctgttcggct ctcccgctct cgcgctgaga tcgagccgca gcgttcccat
      781 cgtatcggtg tcactcgcgt ttgctcgcga ggcgatcgcg catccactcg acgcgaccgg
      841 gttcatcacc ggattcgggg tcgagctcga cgactgcgtg aaatgcagct tcgtatcttc
      901 tacttttcac ggccgcgcgc cggacggcag cgtgctcgtg cggatctttc tgcgccccaa
      961 cgaagccgac ttggaagcgc cgctcgagct ctttcgcgaa cgcgcgacga aggtggcaat
     1021 gcgtgtccta gaagcgaacg ctgccccgca gagagcctgg gaagctcgct ggcgggaggg
     1081 attcgccgtg ctggacgaag agcaactgcg ccgagttgct cttctcgagt acacacttgg
     1141 aagaatgccg atcgcgctcg ctggtacggc cttccacggg gacggcgtcg acggcgccgt
     1201 ccgctccgcc gacaacgccg cgcgcaagct ccgccgccta tgctgaccag gcagcaaagt
     1261 ctcgttctgc atcgccacgc gccgcgagct cgcgaaacgc gctcggcgtg atgagctcga
     1321 acccgctcgg gctcgtccgg agcaagccct gcttctgcca agccgaaacg acgcggataa
     1381 cgctctccat gcacgagcca acgagcaagg acagctcgcg gcgcgagagc gaaaccggga
     1441 tgaccagaga gccttcctcg agctcgtcgc cgaagcgctc tccgagcgtc aagaacaggc
     1501 gagcgagtcg ccgcgaaacc gagcccgcgc tcagcacgtc gaccttggtc gtaaggacgt
     1561 ccacgtagtt cgaactcgcg cgaagaaggg cggcggccaa gcgcgggtcg cgcgcaatac
     1621 cttcgtgcaa gacgttgact ggaatccgca gatactcgac ggagtcactc gccgcgtacg
     1681 ccgttccctg gtgtgtggcg ccaggggcca acgacaacat gccgacacac tcgcgcggac
     1741 cgaacaatcc caagatgttg agctcgatgt ctggagtcgc gcgggtgagc ttcacgatgc
     1801 ccgacgtgac caagcaaagt gcatcagccg gcgttccttg ctcgaagagc gcagttcccc
     1861 gcactctcct cgcgcgcaag gtttgcgcgc cgagctgcag gagtagcgct ggatcgctgc
     1921 cggagaaagc atgcgagacc gccagcaacc tgtcgatccc gccaaatttg cctgagatgg
     1981 gcttcgtagt catttcgatg gaaacgaaag tgcaagcggt gtgcctcgtg acgggcgttg
     2041 atttggcgag ggttcgacga atgggggcgg gggcctgagt tcgggtcgat tcgtctcgtt
     2101 cggcctcgcg cggatgccgc gcctcgctag agaatggctc gatttggggc agcgctcgat
     2161 tcgaggccgc gctcgaggcg tcccaagctc tccgccctcg accaactgca tcgatcgtgg
     2221 attcagcgct cgcttgggag cgccgaacgc ggaatctgcg gccttctaag cagatccagc
     2281 gcgaggtatg ttcccgcttg aatccactcg agcgagctcg gatgtaagcg cccgaatctg
     2341 ctcaccctcg gtggttccga gaccgcgatg aacgacgaga cgaatgtccc cccaagcgtc
     2401 gagccgcagt tgaacgctcg ggtcggcgtc agacacgagt cgctcgaagc cgagcctccc
     2461 gcggagccgc acagtcgcat atgggagcgc tgggccctca aactcacttc gccggcgagc
     2521 gttctcgtca gcgttccgct ctcggccttc gtcgtcggca gctcgctggt actcctgagc
     2581 taccagcgct tcgtgagcct agacgagcga gcagcactcg atcgggcgga gctccgccgg
     2641 gaccaagcgg agcttgcgct gcagcgttcc ctcgccaccg cagatcctct gctcgatcgc
     2701 gtccgcgacg tcgctcgcgc cgcggcaacg agcagtatcg aaacgtcagc gttggccttg
     2761 gcaaatctgg caatccagcg cgaaggtctc acctggctga gcatcagcct ccccgacgga
     2821 agctttcgcg gcgtgtttcg agacaaggac ggctcgctca gctttcaaga gagccgaatc
     2881 gtcgacggt
//
LOCUS       DASTKS010000246         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1154408, whole genome shotgun sequence.
ACCESSION   DASTKS010000246 DASTKS010000000
VERSION     DASTKS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154408"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1389)
                     /locus_tag="VFQ35_05120"
     CDS             complement(178..1389)
                     /locus_tag="VFQ35_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9930040.1"
                     /translation="MQVLLAAVGTRGDVQPALALALELRELGHGVRLCTSPNFLGWAE
                     SLGIDAVPMGVEMRQPAKGASTMPKLTPEELRRLRASLPDLVTDQFDTLAAAAKGCDL
                     IVGANAHQYAAPSIAERARIGYVTTVYAPVAVPSLELAPPPLPGQEASAGSITERWRD
                     VAAAWNERALERVNANRVRLELPPVEDVLEYVLTKHTWLAADPVLAPLPTTPGRTLYQ
                     TGSWVLRDPRPLPPELETFLERGEPPIFVGFGSMPAAAEVSTALVGAARAVGRRLVVS
                     KGWANLELAHEAADCITVEDVSHERLFPRVAAVVHHGGAGTTAAAARAGVPQVITPMF
                     SDQFYWANRVVELGVGATTPHASLSEASLADALASVLAPPIRERARSLTDCLSSDGAR
                     RAARALADEYG"
     gene            complement(1497..1796)
                     /locus_tag="VFQ35_05125"
     CDS             complement(1497..1796)
                     /locus_tag="VFQ35_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930041.1"
                     /translation="MATPDDALNDVYDHVGGTELSSKSATKAIGHESDFVKEQVVLAL
                     RNEARLRPAGDIAVSVTEDEIVLSAPWTDEEHRALSERIARSLAVGRKVRSEPAR"
     gene            complement(1780..2592)
                     /locus_tag="VFQ35_05130"
     CDS             complement(1780..2592)
                     /locus_tag="VFQ35_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930042.1"
                     /translation="MNDEFQSWLLPATPPSFEELLASVRFDDLAKGRRGAVLVNPDSS
                     GVPIVRTTTSYRASAQTFQSIHLRLAESIRERGSLPQRFNNALVEHYTHAYSTMKRHS
                     DQAQDLADGSFIAVYSCYRDPERPSRQLLVQSKEHGDHFAAPLDHGRVVAFSLATNRR
                     FTHAIRLRALAPDNDWLGITFRTSKTLLCFVDGHPVLPSGAHLTLATEDQRRELFQMR
                     RRENEESGFVYPSLSYTLSESDLLPPSTGTAVYPPGTGVASCWSEASNGNSG"
     gene            complement(2589..3770)
                     /locus_tag="VFQ35_05135"
     CDS             complement(2589..3770)
                     /locus_tag="VFQ35_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930043.1"
                     /translation="MGETRSEATLFDAGNLLVAAAGERLGFDFFGEVLDQLPTDVSGK
                     TLYLTGDVANFRGRLDGAARVFAVEKLAWNHGSLETVRLGRLPVRIGDVGVLYRRFFD
                     ETDLFHRITSEHTFQTLTESVKPSKAHRTGIYLTPVTQRGEELHFKLLRCSTNLSGPT
                     ENFCATDWHIVNALNQEANAIFRGHAPLNHVLAQLYPSTPATEDRKQTKARISAHADK
                     TKDMRENGVMAFCTFYDGMGELTPLSADSFDCGYKGRSGLTKLHFRLKEPVRQRVDLR
                     SEFSITLYPGSVFFMPLSTNRLYTHELQPSILNAEMLPTRLGYVVRCSKTTAVHVHGR
                     TFLDVDGERVPMERPTQEGMDALRELYAEENRTDAFVDYGNRFRFSMNEGDYKAPDPK
                     A"
     gene            complement(3859..>4242)
                     /locus_tag="VFQ35_05140"
     CDS             complement(3859..>4242)
                     /locus_tag="VFQ35_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930044.1"
                     /translation="GLGLTADEREAGVAVGMPWTTGGGFGFQLTPNLHVLLEAKVHRY
                     EVRDAFGGELGYTSLTLGPGVFYDLYLWKGLFLQPNVRWWPTVASTYDAPRALRAENG
                     TEYRHARHDLMPFVNMNLGWTFAGK"
BASE COUNT          767 a         1351 c         1354 g          770 t
ORIGIN      
        1 ttcacgtcgt gcgagcggcg aggtcggcgc tggactgtag ctcaccgctc gcggggcgca
       61 gcgtgatcga tgacccgatg cccgttccga aggccgccca ggatgctctt tggggcgaac
      121 tcgacggacc agaaatcacg ctgtggacac cgcatccgtc gcgacggctg tgctccgtca
      181 tccgtattcg tccgcgagcg ctcgcgctgc tcttcgagcc ccatccgagc tcaagcaatc
      241 ggtgagcgag cgggcgcgct cccgaatcgg cggcgcaagc acacttgcaa gcgcgtcggc
      301 gagcgacgct tcgctcagcg acgcgtgtgg agtcgttgct ccgacgccca gctcgaccac
      361 acgattcgcc caatagaatt gatcgctgaa catcggggtg atgacctggg gcactccggc
      421 gcgcgccgcc gccgcggtcg tgccggcgcc tccgtgatgc acgacggcag cgacgcgcgg
      481 gaacaagcgc tcgtggctca cgtcctctac cgtaatgcag tccgccgctt cgtgcgcgag
      541 ctcgagattg gcccatcctt tcgaaacgac gagccgacgc cccaccgcgc gcgcggctcc
      601 gaccaatgcc gtgctcacct ccgccgccgc gggcatgctg ccaaatccca cgaaaatggg
      661 cggctcgccg cgttcgagaa aggtctcgag ctccggggga agcggtcgcg gatcgcggag
      721 cacccaactg ccggtttgat agagcgttcg tccaggggtc gtgggcagcg gcgccaggac
      781 cggatccgca gcgagccagg tatgcttggt gagcacgtac tcgagcacgt cctcgacggg
      841 tggcagctcg agccgaacgc ggttcgcgtt gacgcgctct agcgcgcgct cgttccaggc
      901 ggcggcaacg tcacgccagc gctcggtgat gctccccgcg ctcgcttcct gacccggtaa
      961 gggaggcgga gcgagctcga gcgatgggac ggcgactggg gcgtagacag tggttacgta
     1021 gccaatgcgc gcgcgttcgg cgatcgacgg cgccgcgtat tgatgcgcgt tggcgccgac
     1081 gatcaggtcg cagcccttcg ccgcggccgc gagcgtgtcg aactggtccg ttacgaggtc
     1141 gggcagggat gcgcgcaagc gccggagctc ctccggtgtc agcttcggca tcgtgctcgc
     1201 gccttttgcg ggctgccgca tctcgacgcc catcggcacc gcgtcgatcc cgaggctctc
     1261 cgcccaaccg aggaaattcg gtgaggtgca gagccgcacg ccgtggccca gctcacgcag
     1321 ctcgagcgcc aatgcgagcg ccggctgaac atcgcctcgc gttcccaccg ccgccaacag
     1381 gacttgcatg cattcctcca gtgcttcgcc ctactcccaa aaaaagcagt cgaagcgcac
     1441 accgtagcag aatggcgcgt cgtgcagctt gcgtgcggag ctcgttggaa cggccgctac
     1501 cgagcgggct cggagcgtac cttccttccc accgcgaggg agcgagcaat gcgctcgctg
     1561 agagcacggt gctcctcgtc ggtccaaggc gcgctcagca cgatctcgtc ttccgtcacg
     1621 ctcaccgcga tatcgcccgc gggtcgcagc cgagcctcgt tcctcaacgc gagtacgact
     1681 tgctctttaa cgaagtcgct ctcgtggccg atcgcctttg tggcggattt gctagatagc
     1741 tcggtcccac ccacgtggtc gtagacatcg ttcagagcat catccggagt tgccattcga
     1801 agcctccgac caacaggatg caactcccgt tcccggaggg tagaccgcgg tgcccgtcga
     1861 cggcgggagt aggtcacttt cgctcagcgt gtacgaaagc gatgggtaca cgaagccgct
     1921 ctcctcgttt tcgcgtcgcc tcatctgaaa cagctcgcgg cgttggtctt cggtcgcgag
     1981 cgtcaggtgg gcaccgctgg gcagaaccgg gtgtccatcg acgaaacaga ggagggtctt
     2041 cgacgtccga aacgtaatcc caagccagtc gttgtccggc gcgagagcgc gcagcctgat
     2101 cgcgtgagtg aagcgacggt tggtcgcgag tgagaacgcg acgacgcggc cgtgatccag
     2161 cggagccgcg aaatggtctc cgtgctcttt cgactgcacc agcaactgac gcgacgggcg
     2221 ctcgggatcg cggtagcacg agtagaccgc gatgaacgag ccgtcggcga ggtcctgcgc
     2281 ctggtcggag tggcgcttca tcgtcgagta cgcatgggtg taatgctcga cgagggcgtt
     2341 gttgaacctc tgcggcagtg agcccctttc gcggatcgac tccgcgagcc gaaggtgaat
     2401 ggactgaaat gtctgggccg aagcgcggta cgacgtcgtg gttcggacga tcggaacgcc
     2461 gctcgaatcg ggatttacga gcaccgcgcc acgccgtccc ttcgcgaggt catcgaaacg
     2521 caccgaagcc agcagctcct cgaacgaagg gggagttgcc gggagcagcc aggactggaa
     2581 ctcgtcgttc atgctttggg atccggcgcc ttgtaatcgc cttcgttcat gctgaatcgg
     2641 aagcggttgc cgtagtcgac gaaggcgtcg gtgcgattct cttccgcgta gagctcgcgg
     2701 agcgcgtcca ttccctcctg tgtgggtcgc tccatcggga cgcgctcgcc gtcgacatcg
     2761 aggaaggtgc gaccgtgcac gtgaacggcc gtcgtcttcg agcaccgcac gacatagccg
     2821 aggcgggttg gcagcatctc cgcgttcagg atcgatggct ggagctcgtg ggtgtaaagc
     2881 cggttggtcg agagtggcat gaagaacacc gagcccggat acagagtgat cgagaactcg
     2941 gagcgaaggt cgactcgctg ccgcacaggc tccttcaagc gaaaatgaag cttcgtgagg
     3001 ccgctcctcc ccttgtagcc acaatcgaac gaatcagccg aaagtggggt cagctcgccc
     3061 atgccatcgt agaatgtgca aaacgccatg acgccgttct ctcgcatgtc tttggttttg
     3121 tcggcgtgcg cggagatcct tgctttggtc tgcttgcggt cttcggttgc gggcgtactg
     3181 ggatagagct gcgctagcac gtgattcagc ggcgcgtgcc cacggaagat ggcattggct
     3241 tcctgattca aggcatttac gatgtgccaa tcggtcgcac agaagttctc ggtcggtccc
     3301 gagaggttcg tggagcagcg gagcagcttg aagtgcagct cttccccgcg ttgggtcacc
     3361 ggcgttaggt agatgcccgt ccgatgggcc ttggagggct tcacggattc ggtgagggtc
     3421 tgaaaagtgt gctccgaggt gatgcgatga aataagtcgg tttcatcaaa gaagcggcga
     3481 tacaagacac ccacgtcgcc gattcgaacg ggcaggcgcc cgaggcgcac cgtctcgagc
     3541 gagccatgat tccacgccag tttttcgacc gcaaagacgc gggcagcgcc atcgaggcga
     3601 ccacgaaagt tcgcaacgtc gccagtgaga tagagcgtct ttcccgacac gtccgtgggt
     3661 agctggtcca acacctcacc gaagaagtcg aacccgaggc gctccccagc ggccgcgacg
     3721 agaagattgc ccgcgtcgaa cagagtagcc tcgctccgtg tctcacccat gcttcattca
     3781 ggtagcactg atcgtgagct tcgctaccga gcgtttgcgc gaaaagcgtc cccattcgtg
     3841 cgccccgtgc cgtgtccatt acttccccgc gaatgtccag cccaagttca tgttcacgaa
     3901 cggcatgagg tcatgccgcg cgtgccggta ttcggtcccg ttctccgctc tcaaagcccg
     3961 cggcgcatcg tacgtcgacg caaccgtcgg ccaccaacgc acgttcggct gcaagaacag
     4021 tcccttccac aggtagaggt cgtaaaacac accgggcccg agcgtgagcg acgtgtaacc
     4081 gagctctccg ccgaacgcat cccgaacctc gtagcgatgc acctttgcct ccagcaatac
     4141 gtgcagattg ggagtgagct ggaaaccgaa gccgccgccg gtcgtccatg gcattccgac
     4201 tgcgacgcct gcctctcttt catcggcagt cagccccaaa cc
//
LOCUS       DASTKS010000247         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1154485, whole genome shotgun sequence.
ACCESSION   DASTKS010000247 DASTKS010000000
VERSION     DASTKS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154485"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFQ35_05145"
     CDS             <1..708
                     /locus_tag="VFQ35_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930045.1"
                     /translation="DVKGRELGADIQRLSQHAGSRTLRLIEVEGIISPFCGIGADAQV
                     LTDYNRVKDRLARTPLKRIAPGPLSYGVAAVTRSLPHQLFGKMPHCRIVNMGGPALRL
                     GHKGSVVGRPIERGEVVYEGPIRLAALSTIPYYGYGFRMFPYAEERPDRMHLRATTIS
                     PFAFVTHFKDVWKGRYEDANAVFDYLVEDVQISVDPATPFQIGGDLQGERSELRVGLC
                     SDPIELVDLYAPPSVDA"
     gene            701..1132
                     /locus_tag="VFQ35_05150"
     CDS             701..1132
                     /locus_tag="VFQ35_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930046.1"
                     /translation="MRERSAGGLLGAALLLAALSARADTEQLGVKLNCEPALSVGRIR
                     CALDVSSPEGSRLAWVDALVVETPPFARALRTRVAQPIGVDRTKSELLLGLVASGEGV
                     GTLGVRARAVVCPISGKARCRPLSRLVTLELKVGASPPPPP"
     gene            1186..1890
                     /locus_tag="VFQ35_05155"
     CDS             1186..1890
                     /locus_tag="VFQ35_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9930047.1"
                     /translation="MNLPGRLRSTTLGDLFGQLHRARADGILELVEATGAGAGRAHRV
                     FFAAGLVDDVETSLPAPRLGELLSREGVLAREGLSRLARRLVTEPHRRSGEILVEEKL
                     ASGDDVVSGLRRQLCLRIEALFRLSEALIRFHVRAPRNAAERRPAALGPEEFLHDRPR
                     ARKTRGVRSVPNEAAFRALGLGPGADDEAVQRAFRRLARDVHPDRHPGASAEERARLL
                     SRFAELSQAYHTLTRQ"
     gene            complement(1877..>2410)
                     /locus_tag="VFQ35_05160"
     CDS             complement(1877..>2410)
                     /locus_tag="VFQ35_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930048.1"
                     /translation="HERMVVTLGWAKAGASAGAALVFPTKELAQTVASCGSTMIFSGP
                     MQPAQLGASIASARIFLSPELTTLQQDVEARIALFDGEAAREGLNLRAHGTSPIRFVE
                     TGSEEGAINLAAQLQRSGYFVNAAVCPAVPRGKAGIRMMLTRQQTFEDVRGLVREIGN
                     RTRNRTSRSREMPATGA"
BASE COUNT          363 a          796 c          825 g          426 t
ORIGIN      
        1 gacgtgaaag gccgagagct cggcgccgac atccagcggc tgtcgcagca cgccgggagc
       61 cgtaccctgc ggctcatcga ggtggagggc atcatctcgc cgttttgcgg gatcggcgcg
      121 gacgcccagg tgctcaccga ctataaccgc gtcaaagacc ggctcgctcg cacgccgctg
      181 aagcgcatcg ctcctggccc gttgagctac ggcgtcgctg ccgtgacgcg ttcgttgccg
      241 catcagctgt tcggcaagat gccgcactgt cgcatcgtca acatgggagg gcccgcgttg
      301 cggctcggtc acaagggcag cgtcgtcggg cgtcccatcg agcgcggcga ggtcgtctac
      361 gaggggccga ttcggctcgc ggcgctctcg acgattccct attacggcta tggttttcgg
      421 atgtttccgt acgccgaaga gcggccggat cgcatgcatc ttcgcgcgac taccatctca
      481 ccgttcgcgt tcgtcacgca cttcaaggac gtctggaagg gccgctacga agacgcgaat
      541 gcggtgttcg actacttggt cgaggacgtg cagataagcg tcgatcctgc gacgcctttt
      601 cagatcggcg gggatctcca gggcgaacgc agcgagctgc gcgtcggcct gtgctcggat
      661 ccaatcgagc tcgtcgacct ctatgcgccg ccgagcgtcg atgcgtgagc ggagcgcggg
      721 ggggctgctc ggggctgcgc tcttgctcgc ggcgctctcc gcacgcgccg acaccgaaca
      781 actcggcgtg aagctcaact gtgagccggc gctctcggtc ggccggatcc gctgcgcgct
      841 cgacgtgagc tcgcccgagg ggtcgcgctt ggcttgggtc gatgcgctcg tcgtggagac
      901 accgccgttc gctcgcgcct tgcggacacg ggtcgcgcag cccatcggcg tcgatcgaac
      961 gaagtcggag ctcctgctgg gtctagtcgc gagcggtgag ggggtcggaa cgctcggggt
     1021 gagggcacgc gccgtggtct gcccgatttc cggcaaggca cgctgtcgcc cgctctcgcg
     1081 gttagtgact ctggagctga aagtgggggc ttcgccaccc ccgccgccct gaacggaacg
     1141 gaatttggcc gttcagcggc cgccgggtac ggtggccggg ccccgatgaa tttgcctggc
     1201 cgcctccgct ccacgacact cggcgacttg ttcgggcagc tccaccgagc tcgtgcggac
     1261 gggattctag agttggtcga ggcgacgggc gctggcgccg ggcgagcaca ccgggtgttc
     1321 ttcgcggcgg ggctggtcga cgacgtcgaa accagtctgc ccgcgccgcg gctcggagag
     1381 ctcttgtcac gagagggcgt attggcgcgc gaggggttga gccggctcgc gcgaaggctc
     1441 gtcaccgagc ctcatcgccg ctcgggtgag atcctggtcg aagagaagct agcctccggt
     1501 gacgacgtcg tctcggggct gcgacgtcag ctctgtttac gcatcgaggc gctatttcga
     1561 ctttcggagg ccttgattcg ctttcacgtg cgagcgccgc gcaacgccgc cgaaagacgt
     1621 ccggcagcgc tcggccccga agaatttctg cacgatcgtc cacgggctcg caagacgcgc
     1681 ggcgtgcgta gcgttccgaa cgaagccgcg tttcgcgcgc tcgggctcgg ccccggcgcc
     1741 gacgatgaag cggtgcagcg cgcgtttcga cgtctcgcgc gcgacgtgca ccccgatcgt
     1801 catcccgggg cctctgcaga agagcgagcc cggctgctct cgcgtttcgc agagctcagc
     1861 caggcctacc acaccctcac gcgccagtag ccggcatctc acggctccgc gaggtgcggt
     1921 ttctcgtccg attgccaatt tcacggacga gaccgcgtac gtcctcgaaa gtctgttggc
     1981 gcgtcagcat catgcgaatt ccggctttgc cgcgcggcac cgcaggacac accgccgcgt
     2041 tgacgaaata gccgctgcgc tgcagctgcg ccgcgaggtt gatggcgccc tcttcggagc
     2101 cggtctcgac gaagcggatc ggtgatgtgc cgtgggctcg caggttcagg ccctcgcgcg
     2161 cggcctcacc gtcgaacagg gcgatgcgcg cctccacgtc ttgttgaagt gtcgtcagtt
     2221 caggcgatag aaagatgcgc gccgacgcga tgctcgcgcc gagctgagcc ggctgcatcg
     2281 gacccgaaaa gatcatcgtg ctgccgcagc tcgcgaccgt ttgagcgagc tccttcgtcg
     2341 gaaacacgag cgccgcgccg gcggaagcgc ccgccttcgc ccagccgaga gtgacgacca
     2401 tgcgctcgtg
//
LOCUS       DASTKS010000248         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1824405, whole genome shotgun sequence.
ACCESSION   DASTKS010000248 DASTKS010000000
VERSION     DASTKS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824405"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1649
                     /locus_tag="VFQ35_05165"
     CDS             162..1649
                     /locus_tag="VFQ35_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET9930049.1"
                     /translation="MTAAEVTRGFHSVSPIGSATSIVLPVALSDEELLVATRRLVGRS
                     NQLLALLLGHLAEVEARGLHRIRACSSLYAYCIYELRFSEDEACRRVAAARLVRRFPV
                     LLDVVAAGELHLTGLLMLGPHLTSENLSEVLARSKHRTKKEIARLVRQLDPLPDVPAR
                     IEPLGPAPARTIPMTPTFSQFVESLCPVRELRAGDRPRDWIGDGAKQAGHVEQVEQAG
                     QVEQVEQAEQAEQVEQVEQVEHWREGSNDIGVPDVHDAAPAWLEPQRYRVQFTAGEEY
                     VKLVEAAQALLSHSAERIGLDELQLRAMRALVAELQKRKHAAKKRPTSATAANVSATA
                     LPVSAATGATVSATALPGNSERAARSPRLIPESRAQPVRAGSSEPPGSTVCEIGNCGS
                     FRKARLRQRSRHVPAAVRCAVFERDQGRCAYIDGSGRRCRETRRLELHHVKAFALGGE
                     HTLENLALRCRAHNALAAEQDFGAEFIQRTRDSVRHESRRHQSTR"
     gene            2236..2826
                     /locus_tag="VFQ35_05170"
     CDS             2236..2826
                     /locus_tag="VFQ35_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930050.1"
                     /translation="MAAVSRKPASRASVSSGALLSLQEVDEFQGSDSVGAFWQKLLEL
                     FVGPICDSFSVGGCQAMADLAKVEIFADWQPALKDSTVVRFQWDVAAWERTRDLRLGR
                     FALGGDVVERLGRLPFAGFEREMGCSFARLFFFSRQRLILEAEPGEQSLVFYEMTPAR
                     RELLHDIEPGAARALNDSGNWTATTLISVAALQARA"
     gene            complement(2950..>3435)
                     /locus_tag="VFQ35_05175"
     CDS             complement(2950..>3435)
                     /locus_tag="VFQ35_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9930051.1"
                     /translation="PPGLDFPLTKGWTTTLNIYSQVGLVTLVGLVSKNGILIVEFANH
                     AQSLGMDKIAAVQEAASTRLRPILMTTIATVFGHFPLTLVSGPGAVARNSIGVVLVGG
                     MTIGTLFTLFVVPSVYVLIAQDHRKKLGLGAPSEPPAPNPTPSSIPPEPDLDFEPELV
                     R"
BASE COUNT          605 a          996 c         1205 g          629 t
ORIGIN      
        1 tgtgctccgc gcggcgacgc gattttctgt tccttacggc gcttgatcga ctcgttacgc
       61 cgccctggtg cgtgcggaga gctcgaaagt cgtcgtggtg cgtatgagct cacgagagct
      121 cgaacgcaga gcactgaaaa actgggtgta agttcccacg catgaccgca gccgaggtga
      181 ctcgcgggtt ccattctgtc tctccgatcg gttcggccac ttccatcgtg ctgccagtcg
      241 cgctctccga tgaggagttg ctcgttgcga cgcggcgtct cgtgggccgc tccaatcagc
      301 tgctcgcgtt gctgctcggc catctcgccg aggtagaagc tcgcgggctc caccgcatcc
      361 gcgcttgctc gagcctttat gcatactgca tctatgagtt gcggttttcg gaggacgaag
      421 cctgccgccg cgtcgctgcg gcgcgcctgg ttcggcgctt tccggtattg ctcgacgtgg
      481 tcgccgccgg cgagcttcac cttactggtt tgctgatgct cggcccgcac ctcacctccg
      541 agaatctgag cgaagtgctt gcacggtcga agcaccggac caagaaggag atcgcgcgcc
      601 tcgttcgaca gctggaccct ctgccggacg tcccggcgcg catcgagccg ctcggacccg
      661 cgccggcgcg gacgattccc atgaccccga cgtttagcca gttcgtcgag tctctgtgcc
      721 ccgtgcgcga actcagagct ggtgatcggc ctcgtgattg gatcggagac ggagcgaagc
      781 aggccgggca tgtcgagcag gtcgagcagg ccgggcaggt cgagcaggtc gagcaggccg
      841 agcaggccga gcaggtcgag caggtcgagc aggtcgagca ctggcgcgaa ggatcgaacg
      901 atatcggtgt accggacgtg cacgacgcgg caccggcgtg gctcgaacct caacgctatc
      961 gcgtacagtt cactgccggc gaggagtacg tgaagctcgt cgaagcggct caggcgctgc
     1021 tctctcactc cgctgaacgg atcggtctcg atgagctcca gctccgggcg atgcgcgcac
     1081 tcgtggccga gctgcaaaag agaaagcacg cggccaagaa gagaccgacc tcggcgacgg
     1141 cagccaatgt gagcgccaca gcgttgcccg tgagcgcggc gacgggagcc actgtgagcg
     1201 ccacagcgct gcccgggaac tcggagcgcg cggcgcggag ccctcgacta atacctgaat
     1261 cgagagcgca acctgttcgt gcaggctcga gcgagccccc aggctcaacc gtctgcgaga
     1321 ttggcaattg tggcagcttc cgaaaagcgc gcctgcgcca gcgcagcaga catgtgcccg
     1381 ctgccgtgcg atgcgcggtc ttcgagcgtg accaggggcg ctgtgcgtac atcgacggct
     1441 cggggcggcg ctgccgggag acgcggcggc tcgagcttca ccacgtgaaa gcattcgcgc
     1501 tgggcggcga gcacacgttg gaaaatcttg cgctacgctg ccgcgcgcac aacgccctcg
     1561 ccgccgagca ggacttcggc gcagagttca ttcagcgaac gcgcgactcg gtacgacacg
     1621 aatcgcgcag acatcaatct acccgatagg agcagcatcg aggcgccgtt ccaactccgc
     1681 gaaggaggga gactggagtg tcggcacgcg gtcgatttgg ggacgatgct cgtggcgctt
     1741 tgcggtggtc gaagcggcca ttcgcgcagc tcgtgccgca atgtaaagat gctttcgatt
     1801 gcggcgaccc cgggctcgat tctcgttcga acggctgctc cctagtcgga atggccgagc
     1861 cggtggacgg gagcacggat aaactggatt tttgctgtca acgcgtctga caagagtttc
     1921 gtaaacccag ttgcgttcga atgatgtgag ccgaaaccgt gctcgcgtga catcttgggt
     1981 gtgaagaaat ggctgcgttc gtgatcgacg ataccggttg cgaggttcta acggcttcac
     2041 ctgcgatgcg ctcagagcga gcggacctcg ctttgcgaat cggcggcgtg cgaatgagca
     2101 gcgcgcgaat cagcagcgcg cgaatcagca gcgcgcgagt cagcagcgcg cgagtcaggg
     2161 gtgcggtagt gacggatacg ggagtgagga tatgagggtg ggctcgatgg agaagtattg
     2221 gggtagaggt tcatcatggc agcggtaagt agaaagcccg cttcgagggc cagcgtatct
     2281 tcgggtgcgc ttctgtcgct ccaagaagtc gatgagttcc agggcagcga ctcggtcggc
     2341 gcgttttggc agaagctgct cgagctgttc gtaggcccga tctgtgacag cttcagtgtc
     2401 ggtggctgcc aggcgatggc cgacttagcg aaggtcgaga tctttgccga ctggcaaccg
     2461 gcactgaagg actcgacggt ggttcgtttc caatgggacg tggcggcctg ggagcggact
     2521 cgagacctcc gccttgggcg tttcgcgctc ggcggcgacg tggtggagcg acttgggcgg
     2581 ctcccgttcg ccggcttcga gcgtgaaatg ggatgctcct tcgcccgatt gttcttcttt
     2641 tctcggcagc gtttgatctt ggaagccgag cccggcgaac aatcgctggt cttttacgag
     2701 atgacgcccg cgcgtcggga gctattgcac gatatcgaac cgggggcagc gcgcgcgctg
     2761 aacgattccg gcaattggac cgcgacgact ctcatcagcg ttgcagcgtt gcaagctcgg
     2821 gcctaaatag aggcgccacg cggaggtccg gagagcgtcg cgcggcgttc agcgacggag
     2881 agcgtcgcgc gcggcgttca gcaacgggag agcgtcgcgc gcgtggctca gagagcgtgt
     2941 ggtgccctat cagcgtacga gttcgggctc gaagtcgaga tccggctctg gggggattga
     3001 gctcggggtg gggttggggg cgggaggttc ggagggggct ccgaggccga gcttcttgcg
     3061 gtggtcttga gcgatgagga cgtagacgga gggcacgacg aacaaggtga acaaggtgcc
     3121 gatggtcatg ccgccgacga gcacgacgcc gatggagttg cgggcgactg cgcccgggcc
     3181 cgatacgagg gtgagcggga agtggccgaa gacggtcgcg atggtcgtca tcagaatcgg
     3241 ccgaaggcgc gtgctcgcgg cctcttgcac cgccgcgatc ttgtccatgc cgaggctctg
     3301 cgcatggttc gcgaactcga cgatgaggat gccgttcttc gacacgaggc ccacgagggt
     3361 gacgagcccg acttgcgagt agatgttcag cgtggtcgtc catcccttgg tgagcgggaa
     3421 gtcgagcccg ggcgg
//
LOCUS       DASTKS010000249         5374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_707886, whole genome shotgun sequence.
ACCESSION   DASTKS010000249 DASTKS010000000
VERSION     DASTKS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707886"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..626
                     /locus_tag="VFQ35_05180"
     CDS             144..626
                     /locus_tag="VFQ35_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930052.1"
                     /translation="MRELAFAVIALQALFMLVDEARFHRRRGLPRWERIGHPIDTASV
                     LACCCVTVVFEPTPRSLWLYAALGIGSCLLVTKDEFVHSRHCEPMEHWLHAMLFILHP
                     LLLAALAVLWLQHERVVLTAQTLATFAFGAYQAIYWNAIASPVHRRPSRARVVHQGSE
                     "
     gene            629..1408
                     /gene="ubiG"
                     /locus_tag="VFQ35_05185"
     CDS             629..1408
                     /gene="ubiG"
                     /locus_tag="VFQ35_05185"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HET9930053.1"
                     /translation="MSSTLARPVNNAIYDTLGNRWYDAQDDPVALLRAESRSRNPWVL
                     ERILSSRGPSARVLDVGCGAGLLSNYLAVAGLEVHGLDASQDSLSVAERHDETRRVQY
                     RVGDALALPYADASFDVVCAMDFLEHVEEPAKVVQEASRVLRPNGMFFFHTFNRNFLA
                     WLVVIKGLEWFVKNTPRDLHVLRLFLKPSEVTRFCEDVGLGVRELHGSAPVVFSRAFW
                     QMLFTGVVPLDFRFRFTRSTLIAYTGYATKPTLQLEGVSRT"
     gene            complement(1493..2386)
                     /locus_tag="VFQ35_05190"
     CDS             complement(1493..2386)
                     /locus_tag="VFQ35_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9930054.1"
                     /translation="MMTTTTRSADPAPWLALLVHLRLHYQLVFLSPLFAWGFMLGARR
                     ISVLAAIAFVAFHVFLYGGLTAYNSYYDRDEGPVGGLRKPPPVPESLLAFSLAMQAIG
                     GLMALYVGFAFTLLYAAIATLSVAYSHPRLRWKSKPVGSLLVVAFGQGTAGFIAGWLA
                     SGAAWPPPHPLRLGLGAAVATLATVGIYPLTQIYQIEEDAARGDRTFAVAFGARASFD
                     FSLSCLAAAGACLVALLGTFGKWDAVFGALGFLLLISGLWRWRSQFRSDVAMNFKAVH
                     VAQLGVSVATLGYVAVRMLFN"
     gene            2923..4341
                     /locus_tag="VFQ35_05195"
     CDS             2923..4341
                     /locus_tag="VFQ35_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9930055.1"
                     /translation="MIGLVALLVACGKGESNGEGAGGAKVGSGGGASPNGGSASNSGG
                     QSTGSDRGGAPASGGASNAAGGSSSDAAGGRVMQAMGGTASGGAATGGGQGGKSSDGG
                     RSASSDGGRTANGGAGGAASGGASAGGGAAGASSATATSCSKSGPGLNTCGKSKDESC
                     CTTLPVPGGMFYRTYTNSGSGATAKADPATISTFRLDKYEVTVARFREFVKYLDGGGM
                     PPAAGSGKHTHLNEGKGLADSGKSGSFESGWDSSWNSKIPSGAGAAAKWKSLLTSKGT
                     SGAGCSVYGSFTDQPGDNDLLPVTCTSWYESYAFCIWDGGFLPSEAEWRYAIAGGDEQ
                     RQYAWGTAAPGSKNQYAIYDCCYPSGQCSAAAGHDTCTGLVNAAPVGFAELGVGRYGQ
                     LDLAGSIWEWLLDKYSNSLTSPCTDCAYLTGSTTNRVLPGGGFHTGANPYLLSSNRSG
                     VSYDAETYRGDYAVGVRCARTP"
     gene            complement(4404..>5374)
                     /locus_tag="VFQ35_05200"
     CDS             complement(4404..>5374)
                     /locus_tag="VFQ35_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930056.1"
                     /translation="DADGETEPLLQPFEGVIWASLTDAGGANRFRWGFPAPGYVAWSS
                     PEVDEGPEILKMARVDDPASKVTVATNVHYWRMSPDDSQWFWLSMADSKGNGLLQTAK
                     FPDGEPFDLLSDVRDYGLDSKGALVAITVGGEVVSIPKPLSAPERKLSLDQDAKVLVA
                     VGDEGDVAYGKHVVSGKVVDLFTAKLDGSRRCTLDTTTNVPLNSFRFGPGTEAAVWAL
                     QNNGQYDAYHTRLADCATESMAPAVSMLSWISRGTAMFVDEFDSWSASGSLRVRSVSK
                     SGALEPAPATLLAQRVGSFGTTGSVLLYASSVEADTEGLYVRAFPR"
BASE COUNT          997 a         1695 c         1789 g          893 t
ORIGIN      
        1 ggcaatatgt cgtctgcaac actgccccac atttgggcgg aaatcgcgaa cgactcgagc
       61 gtccgcgcgg gcgccatcat cgtgagtctc gcgttcggac caggacttac gatgtgcggc
      121 gcggttttgc gtaagcttgc cgcatgagag agctcgcgtt cgcggtcatc gcgctccaag
      181 cgcttttcat gctcgtggac gaggcgcgct ttcaccgccg tcgcgggctt cctcgctggg
      241 aacggatcgg gcaccccatc gacaccgcga gcgtgctcgc ttgctgctgc gtgacggtgg
      301 tcttcgagcc gacgccacgc tccttgtggc tgtacgcggc gctgggcatc ggctcgtgct
      361 tgctcgttac gaaggacgag ttcgttcact cacgtcattg cgaaccgatg gagcactggc
      421 tccacgccat gttgttcatc ttgcatccct tgctgttggc cgcgctggcg gtgctctggc
      481 tgcagcacga gcgcgtcgtc ttgacggccc aaacgctcgc gacgttcgca ttcggcgcgt
      541 accaggccat ttactggaac gccatcgcct caccggtgca tcgccggccc tcgcgggctc
      601 gcgtcgttca ccaaggatcg gagtagccat gtcctcgacg ctcgcgcgcc ccgtaaacaa
      661 cgccatctac gataccttgg gtaaccgatg gtacgacgcc caggacgacc ccgtcgcgct
      721 actccgggcc gagtcgcgct cgagaaatcc ctgggttctc gagcggatcc tttcgtcgcg
      781 gggcccttcg gcgcgggtgc tcgacgtcgg ctgcggcgct gggcttctct cgaactacct
      841 cgcggtcgcg gggctcgaag ttcacgggct cgacgcatcc caagacagcc tttcggtggc
      901 ggagcgccac gatgagactc gccgcgtcca gtaccgagtc ggagacgcgc tcgcgctgcc
      961 ctacgcggac gcttcgttcg acgtcgtgtg cgcgatggac tttctggagc acgtcgaaga
     1021 gccggccaag gtagtgcaag aggcgtcgcg cgtactgcgg ccgaacggga tgttcttctt
     1081 ccacacgttc aaccgcaatt tcttggcctg gctcgtcgtc atcaaggggc tcgagtggtt
     1141 cgtgaagaac acaccgagag acctgcatgt cttgcggctt ttcttgaaac cgagcgaggt
     1201 gacgcggttc tgcgaagacg tgggcctcgg cgtgcgtgag ttgcatggca gcgctccggt
     1261 cgtattcagc cgcgcgtttt ggcagatgtt gttcacgggc gtcgtgccac tcgacttccg
     1321 gtttcgtttc acgcgctcca cgctcatcgc gtacacgggc tacgcgacca agccgacgct
     1381 tcaactcgaa ggcgtctcga gaacctaacg tggcggacca gcgcaacgac gagcccgcac
     1441 gccgcagcaa gaggatcggc ccgcgcagta tgacttgtcc attcgcgggc ccttagttga
     1501 agagcatgcg gacggcgacg tagccgaggg ttgccaccga cacgccgagt tgagcgacgt
     1561 gcaccgcctt gaagttcatc gcaacgtcgc tccgaaactg cgaacgccag cgccagaggc
     1621 cggagatcaa caggaggaag ccgagcgcac cgaacactgc gtcccacttg ccgaaggtac
     1681 cgagtaaggc gaccaggcac gcgcccgcag ccgcgagaca gctcagactg aaatcgaagc
     1741 tggcgcgcgc gccgaacgcc accgcaaagg ttcggtcgcc gcgagcagcg tcctcttcga
     1801 tttgatagat ttgagtgagt gggtatatac cgaccgttgc tagcgttgcg acggcagcgc
     1861 cgagcccgag gcggagcggg tgcggtgggg gccacgctgc gccgctcgca agccaacccg
     1921 caatgaaacc agccgtgccc tgaccaaaag cgacgacgag caacgaaccg acgggcttgc
     1981 tcttccaacg tagacgcgga tgcgaatacg cgaccgaaag ggttgcgatg gcggcgtaaa
     2041 gcagcgtgaa cgcgaacccc acgtaaagag ccatcagccc accgatcgcc tgcattgcga
     2101 gcgagaacgc gagcaacgat tcgggcacgg gcggaggctt tcgcaagcct cccacaggcc
     2161 cctcgtcccg atcgtagtag gagttgtacg ccgtgaggcc gccgtacaaa aagacgtgaa
     2221 acgcgacgaa cgcgatggcg gccaagaccg agatccgacg cgcgcccaac atgaagcccc
     2281 aggcaaagag cggagagagg aagacgagct ggtagtgcag gcgcaaatgc acgagcagcg
     2341 cgagccaggg agcggggtct gcgctgcggg tcgtggtcgt catcatgtgt tccccatcga
     2401 gcgcggtcga gcacgggcgt gagcgcgccc aacgttcggc tcctcctgtg caattggcgt
     2461 gcacagcgta catgctcggc ggcgctcccg ctgcttcgcc gacgcggtcg tcgtacacaa
     2521 agccagtagt tgccgagtct gagggggatt cgacggacgt tccggccaat cgagcttgct
     2581 cgggtgggtt gaatcgagcg taggcggcgc gccctgaggc gtaattgtgc gagcatcgac
     2641 gcgtatccgc gagccctccg gcccgaagcg cgagcggcgc ggccctctat tttttcagag
     2701 ctccctcgat ttctgaaaaa ggcttccgct gtcaaccatt ctccgcacgt ggcgattcag
     2761 caaaacttcg gcttggcggc gatgcgcggg tgtcgatgca ttcgtatcgc agagttgaag
     2821 atacactcga gatcttgcga attcgataac gcgcttgccg agcgaacggc cctcgtgcaa
     2881 cttccgacca tgcaacgcac cgcgagtgcc gaaagagttc gtatgattgg cctggtcgct
     2941 ttgctggtcg cttgcggcaa gggcgagagc aacggcgaag gcgccggcgg agcaaaagtc
     3001 ggcagcggcg gcggcgctag ccccaatggt ggcagcgcga gcaactctgg cggccagagc
     3061 actggtagcg accggggtgg agcgcctgct tccggcggcg cgtcgaacgc cgcgggcggc
     3121 agctcgagcg atgcggcggg cggtcgagtg atgcaagcga tgggtggcac ggcgagcggc
     3181 ggagcggcca cgggcggagg gcaagggggc aaatcgagcg acggcggccg atcggcgtcg
     3241 agcgatggcg gccgaacggc gaacggcggt gcgggcggcg cagcgagtgg aggggcctcc
     3301 gcgggcggcg gggcggcggg cgcgagcagc gcgactgcga cgagctgctc gaagagcgga
     3361 ccggggctga acacgtgcgg caagagcaag gacgagagct gctgcaccac tttgccagta
     3421 ccgggcggga tgttctatcg cacttatacg aactctggca gcggcgcgac cgccaaggcc
     3481 gacccggcga cgattagcac gttccgtttg gacaaatacg aagtcacggt cgcgcgcttc
     3541 cgcgagttcg tgaagtacct cgacggcggc ggcatgccgc ccgcagctgg cagcggcaaa
     3601 cacacgcatt tgaacgaggg caaagggctc gccgacagcg ggaagtccgg ctctttcgag
     3661 agcggctggg actcgagctg gaacagcaag atcccgagcg gcgcgggagc ggccgccaag
     3721 tggaaatcgc tactcacgag caaggggacg agcggcgcgg ggtgcagcgt ctacggcagc
     3781 ttcacggacc agccgggcga caacgacctt ttgcccgtca cctgcacgag ctggtacgag
     3841 tcgtatgcgt tctgcatttg ggacggcggc ttcttgccga gcgaagccga gtggcgatat
     3901 gcgatcgccg ggggcgacga acaacggcag tatgcgtggg gcacggcggc ccccggctcg
     3961 aagaatcaat atgccattta cgactgctgc tatccgtcgg ggcagtgcag cgcggcggcc
     4021 ggacacgata cttgcaccgg cctcgtcaac gcggcgccag tgggctttgc agagctcggc
     4081 gtcggacgct acgggcagct cgatctcgca gggagcattt gggaatggct gctcgacaag
     4141 tactcgaaca gcttgacgag cccctgcacc gactgcgcct acctcaccgg cagtacgacg
     4201 aaccgggtgt tgccgggtgg aggcttccac accggcgcca atccgtatct tttgtcctcc
     4261 aatcgaagcg gcgtatcgta cgacgcggag acgtatcgcg gcgactacgc cgtcggcgtt
     4321 cgctgcgccc gcacgccctg aaagaagcag cgcacgcggg cgcgcgcatc gagcgaaccc
     4381 acggcgcggc gcacgagctt tggtcagcga gggaacgctc ggacgtagag cccctccgta
     4441 tccgcttcca cgctcgaagc gtagagcaga acggagccag tggtcccgaa ggaaccgacg
     4501 cgttgggcga gtagcgtcgc cggagccggc tcgagcgcgc cactcttgct cacgctccgt
     4561 acgcgcagag agccggacgc actccagctg tcgaactcgt cgacgaacat cgcggtgcca
     4621 cggctgatcc aactcagcat cgagacggca ggcgccatcg attcggtcgc gcaatcggcg
     4681 agccgcgtgt gataggcgtc gtattgaccg ttgttttgca gcgcccacac ggccgcctcg
     4741 gtacccggcc caaaacgaaa cgagttgagt gggacgttgg tcgtcgtatc caacgtgcag
     4801 cgccgagagc catcgagctt cgcggtgaac agatcaacga cttttccaga gacgacgtgc
     4861 ttgccgtagg caacgtcacc ttcgtctccc actgcgacga gcaccttcgc atcctgatcg
     4921 agtgagagct tgcgctcggg agcgctgagc ggcttcggaa tcgaaacgac ctcgccgccg
     4981 accgtaatcg ccacaagcgc gcccttcgag tcgagcccgt agtctcgcac gtcgctgagc
     5041 aggtcgaacg gctcgccgtc ggggaatttc gcagtctgca gcaagccgtt gcctttcgag
     5101 tccgccatgc tgagccagaa ccactgggag tcgtcagggg acattcgcca gtagtggacg
     5161 ttcgtcgcga ccgtcacctt gctcgcgggg tcgtcgacgc gagccatctt caagatctcg
     5221 gggccctcgt cgacctccgg gctcgaccac gccacgtagc ccggagccgg aaatccccaa
     5281 cgaaatcggt tcgcgccgcc ggcgtcggtg aggctcgccc agatgacgcc ctcgaacggc
     5341 tggagcagag gctcggtctc accgtccgca tctc
//
LOCUS       DASTKS010000250         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1378480, whole genome shotgun sequence.
ACCESSION   DASTKS010000250 DASTKS010000000
VERSION     DASTKS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378480"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..973
                     /locus_tag="VFQ35_05205"
     CDS             101..973
                     /locus_tag="VFQ35_05205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HET9930057.1"
                     /translation="MARQIDPEVTRYIAQVNGFPQLSREEELELLRRWREEQDDDARQ
                     SIVESHLRYVVAISLRYRRYGMPIGELIAEGNFGIVHALDKFDPTRGTRFVTYAAYWI
                     RAYILNHIIRCWSLVGVGSGALRSKMFFKLRRERVRITNLVGEGDQADEMLAQALELP
                     KARVVSMVRRLEARDVSLDAKVFDDSATTLADTLLATDASQEERFGASELGDHLRDLL
                     RDAVAALDKRERYIVEKRLMADSEDELSLAEIGRRLGVSRERARQLEARAKRKLKTRI
                     GDITALKDIWLSAA"
     gene            1106..1493
                     /gene="rnpB"
                     /locus_tag="VFQ35_05210"
     ncRNA           1106..1493
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFQ35_05210"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1543..3300)
                     /locus_tag="VFQ35_05215"
     CDS             complement(1543..3300)
                     /locus_tag="VFQ35_05215"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HET9930058.1"
                     /translation="MKFLCPSCKAKYQISDEKVAGRSVRMKCRKCGHIIQVSSEDAIA
                     GSSAPPPPVEEGDGEVVAKEPAAPSATRNPTSAEKPKAAPGVPRAGAPAPPSAAAKSR
                     VPSPKAAAPSADVAPKPAATAPRAGADKAADALRVPRVASTAAREAKSPAPPVTTGTA
                     ARAPAKPQAAAAASAASPPVAAPTPSPAAVAGAPRPAAPDPANAKLFDDDGEEEATQI
                     ARGALIGAFSAAMKDHAPASVADPLSMPGDEWFVGINGVPVGPIRLSELRAKAASGAV
                     TKESLVWRDGFEEWRPLKSFPELVAIVEESLSSARASMPFPVTAASAGTLADPFAGTA
                     PAPSATPGVGSEGVSFDELQALRARPRTSPAAWLAVVVAVAFGLTLGAVIFGRGPAAQ
                     SSVEKPAEKPANAAPSAAAPTQVAAAEQKPEEQVISGDTKATVKNPSKTTSTKTDSET
                     KTGSKSGGLGLSGLAANGTRGPTDTPGSTSQGGPGGGQLDQTQLSSVVQRYTPSVKRS
                     CWQPALDGRDRDAPTSARVSVAITISPSGSVQDVNASGDPRGYRGLAQCIAGRVRGWQ
                     FPPSGGSTTVNVPFVFVAQ"
BASE COUNT          560 a         1130 c         1168 g          591 t
ORIGIN      
        1 agaacgtctc ttgcagtgcc gcatagaatc cgctgcggcc ccatttgact aaagttcgtc
       61 caggacatat catggacaaa ctcgctcagg taaaggtccc atggctcgac agatcgaccc
      121 cgaagtgact cgttacatcg ctcaagtgaa tggcttcccg cagctgtccc gtgaggagga
      181 gctggagctc ttgcggcgct ggcgcgaaga gcaggacgac gacgcccgac agtccatcgt
      241 ggagtcgcac cttcgctatg tggtggcgat ctccttgcgc taccggcgtt acggaatgcc
      301 gatcggggaa ctgatcgccg agggcaactt cggcatcgtt cacgccctcg acaaattcga
      361 tccgactcgc ggcacgcgct tcgtcaccta cgccgcgtac tggatcaggg cgtacatcct
      421 gaatcacatc attcgctgct ggagcttggt tggagtcggc tccggagcgc tgcgttcgaa
      481 gatgttcttc aagctccgtc gcgagcgtgt tcgcatcacc aacttggtcg gcgaaggcga
      541 ccaggcggac gagatgctcg cgcaggcgct cgagctcccg aaggctcgcg tcgtctcgat
      601 ggttcgccgc ctagaggccc gcgacgtgtc gctcgacgca aaggtcttcg acgactcggc
      661 gaccacgttg gcagataccc tgctcgcgac cgatgcgagc caggaagagc gctttggcgc
      721 gagtgagctc ggagaccacc tgcgcgatct tcttcgtgac gccgtcgccg cgctcgacaa
      781 gcgggagcgc tacatcgtgg agaagcgttt gatggcggac tccgaggacg agctctcgct
      841 cgccgagatc ggccgacgcc tcggcgtgtc gcgtgagcgg gcacgccagc tcgaagcgcg
      901 cgccaagcga aagctgaaga cgcggatcgg tgacatcacc gcgctgaaag acatctggct
      961 cagcgcggcg tgaccaggcg cttcgagcgg cttgattgcc gcgccggagc tggtcgttgg
     1021 gacggggccg agtggccccg ttctgctttt gtgcggctgg atctggtttg cgtgggcagc
     1081 ggagccgctg ctagtgtggc gacgccgcag cggttcaggc gatcgctcgt cgcgctcgac
     1141 gcggcgggag gaaagtccgg gctccatagg gcaggatgcc gggtaacacc cggtgagggt
     1201 gacctcgagg aaagtgccac agaaaacgag aaaccgccga cgcgacggca acgtcccgtc
     1261 ggtaagggtg aaacggtggg gtaagagccc accgcgcgat cggtaacgat cgcggcacgg
     1321 taaaccccat ccggagcaag gccacgtaga ggggcgttcg agggctgccc gcccgagccc
     1381 ctgggtaggc cgcacgaggc gcgcggcgac gcgcgtccca gaggaatgac cgccacggga
     1441 gtgcggggta actcgtctcc cgaacagaac ccggcttacg aaccctgcga ccacatcgag
     1501 cgagcttcgg ctcgctcgac gcgttttgta cgcgggacgc ggttactgcg cgacgaacac
     1561 gaacggcacg ttgacggtcg tcgaaccgcc cgacggaggg aattgccaac cacgcacgcg
     1621 cccggcgatg cactgcgcga gcccgcggta tccgcgcgga tccccgcttg cgttcacgtc
     1681 ctgcacgctg ccgctcgggc tgatcgtgat cgccaccgat acgcgcgccg aagtcggagc
     1741 atctcggtcg cgaccgtcga gtgcgggctg ccagcagctt cgcttcacac tgggcgtgta
     1801 gcgctgcacg acgctggaca gctgcgtttg atcgagctgg ccgccgccgg gtccaccttg
     1861 cgatgtcgaa ccaggcgtat cggtgggacc gcgcgtgccg ttcgcggcca aaccggacag
     1921 gccgagtcct ccggacttgc taccggtctt cgtctcggag tcggtcttgg tcgaggtcgt
     1981 cttgctcggg ttcttgaccg tcgccttcgt atcgccggag atgacctgct cttcaggctt
     2041 ttgctccgcc gcggcgactt gcgttggggc ggcggccgac ggcgccgcat tcgcaggctt
     2101 ttccgctggt ttttcgacgc tggattgtgc ggctgggccg cgaccgaaaa tcaccgcgcc
     2161 cagcgtgagc ccgaacgcga cggccacgac gaccgcgagc cacgcagccg gcgaggtgcg
     2221 cgggcgagcg cgcagcgcct gcagctcgtc gaagcttacg ccttcggaac caacacccgg
     2281 agtcgcgctc ggtgcaggtg cggtgccagc gaaaggatcc gcgagcgtcc ccgcggacgc
     2341 cgccgtcacc gggaaaggca tcgaggcgcg cgcgctcgac aagctctcct ccacgatcgc
     2401 gacgagttcg gggaagctct tcagcggacg ccattcttcg aagccgtctc tccaaacgag
     2461 agactccttc gtaaccgctc ccgaggccgc ttttgcgcgg agctcgctca agcgaatcgg
     2521 cccaactggg acgccgttga tgcccacgaa ccattcgtcg cctggcatgc tcaacggatc
     2581 ggcgaccgac gcgggcgcgt gatctttcat cgccgcgctg aacgcgccga tgagcgcgcc
     2641 gcgggcgatc tgcgtcgcct cttcttctcc gtcgtcgtcg aagagcttgg cgttggcggg
     2701 atcgggcgct gctgggcgag gcgcgcctgc aaccgcggcc ggtgatggcg tcggagccgc
     2761 taccggtggc gacgccgcag aggcagccgc cgccgcttgc ggctttgccg gtgcgcgcgc
     2821 ggcagttcca gtcgttacgg gaggcgccgg cgacttcgcc tcacgcgcag cggtcgacgc
     2881 gacgcggggc acgcgcagtg cgtccgcagc cttgtctgcg ccggcgcgcg gagccgtcgc
     2941 ggcgggcttc ggggctacat cggcactcgg tgccgcagct ttgggagacg ggacgcgcga
     3001 cttggctgcc gcggagggcg gcgcgggggc gccagcacgc ggcactcccg gggccgcctt
     3061 cggtttctct gctgaggtgg gattccgcgt agcgctcgga gcggcgggct ctttcgctac
     3121 tacctctcca tcaccctctt cgaccggcgg tggcggcgcc gacgatcctg caatcgcgtc
     3181 ctcgctggag acctgaataa tgtgaccgca cttgcggcac ttcatccgca ccgagcgtcc
     3241 cgcgaccttc tcgtcgctaa tctggtactt cgccttgcag ctgggacaga ggaacttcat
     3301 tcaaccagac gccgtcggta tcgagcgttt cgggcaggga gaatagctcg aggctagcat
     3361 tcctacttca cttttcagca ggacggcaca gggcgaaaaa tgcgactccg gcccgttggt
     3421 tccagcgctg cccgggcgcg gcgccgacc
//
LOCUS       DASTKS010000251         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1601968, whole genome shotgun sequence.
ACCESSION   DASTKS010000251 DASTKS010000000
VERSION     DASTKS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601968"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1532
                     /locus_tag="VFQ35_05220"
     CDS             99..1532
                     /locus_tag="VFQ35_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930059.1"
                     /translation="MNEHVQEPAAVREASPLIRHRLLTILLLGAIVSALSVGALARLL
                     TVSTQHRIERAREEVNELLSRLAQDGTALSDPAPGNVVGMRAGLSDGRTPLSAPGRWR
                     PELERALKESASTNPRTKQVALESSNLVLGTVRAADGRVGWAALEVRPLPHLETWKFT
                     IWLLAFATIVLVATTMRAAVSMQRDASALGQSLSSLARDLSAPVARPALRELGDVADG
                     IVQLARRLADARREEARLAEELSRNERLAALGRVAAGVAHEVRNPLASIKLRLDLAMA
                     GSRLPDSVRDAISHASAEILRLDRLVADLLVVAGRSTGPQVRLSLSELTRARCEVLRP
                     WAAERHVELRVDGDASVSAHADSLARALDNLIRNAVEASSPESTVAIRISEDEGRAKV
                     SVRDRGPGVEAQRASELFEPFFTTKPDGTGLGLPLARSIAQAHGGDVRYVRVDAETRF
                     EVELPLASENGAPPRSPEAESELRFHS"
     gene            1529..2890
                     /locus_tag="VFQ35_05225"
     CDS             1529..2890
                     /locus_tag="VFQ35_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9930060.1"
                     /translation="MKDQPQSEAPARVLIVEDERGLREGLMGAVERIGCEALAAAGVQ
                     EARQRLAEHEVDCVLLDIRLKDGDGLELLTELRAGPLRDTPVIVATAYSDSERTIRAM
                     RDGAFDYVTKPFDLPALLVTVRRALDQRRLRRAVNDPAEPPRALPQGLVGASAAMLAT
                     WKLIGRAAASSAPVLVTGETGVGKELVARAVHDYSTRVTQPFVAVNLAALPPTLIESE
                     LFGHERGAFTGASGRRVGRFELAAGGTLFLDEIGDLDPSLQTKLLRVLQEGTFERVGG
                     QETLEFGARVVTATNKPVHPGQPGCALREDLYYRLAVVEIEVPPLRARKSDIPLLVAH
                     ALRSTPARAVSEQAMERLLAYAWPGNVRELIHVIERAAVLCGGEIIDVADLPDAVREP
                     VARASAPEPLETSNLREAVARLERRLIAAALVETQGNRSEAARRLGIARAQLYAKIEE
                     YGL"
BASE COUNT          465 a         1022 c         1061 g          494 t
ORIGIN      
        1 gcttcgccga ggccggatct tgcgcagcca aacgcgggga gctccctgcg agtccccatg
       61 gcgtaccctc agtcggctcc caggcaccac gagatgccat gaatgagcac gtgcaagaac
      121 ccgcggcagt acgcgaagca agccccctca tccggcatcg tctactcacg atcttgctcc
      181 tcggggcgat tgtttcggcg ctctcggtcg gagcgctggc gcggctgctc accgtgagca
      241 cgcagcatcg catcgagcgc gcgcgtgagg aggtgaatga gctcttgtcg cggctggcgc
      301 aggacggcac ggcgctttcg gatcccgcgc ccggaaacgt cgtggggatg cgcgccgggt
      361 tgagcgacgg tcggacgccg ttgtcagctc cgggtcgttg gcggccggag ctcgagcgag
      421 cactgaagga gtcggcttcg accaaccctc gaaccaaaca ggtcgcgctc gagagttcga
      481 atctcgtgct gggtacggtg cgggcggccg acggtcgcgt gggctgggcg gccctcgagg
      541 tacggccgtt gccccacctc gaaacgtgga agtttacgat ttggctcctg gccttcgcca
      601 ccatcgtgct cgttgcgacc acgatgcgag ccgccgtttc gatgcaacgc gacgcgagcg
      661 cgctcggcca atcgctctcg agtttggcgc gtgatttgag cgctcccgtg gcgagacccg
      721 ctctgcgcga gctcggcgac gttgccgacg gcatcgtgca actcgcgagg cgcctcgccg
      781 acgcgcgccg cgaggaagct cgcctcgccg aggagctgtc acgcaacgaa cgcttggcgg
      841 cgctcgggcg cgtggccgcc ggcgtcgcac acgaggtgcg caacccgctc gcgtcgatca
      901 agctccgcct agatttggcg atggcgggtt cgcgcctgcc cgattcggtg cgcgacgcga
      961 tcagtcacgc cagcgccgag atcttgcggc tcgatcggct cgttgcagat ctgttggtcg
     1021 tcgctgggcg ctcgacgggc ccgcaggtgc ggctctcgct ctcggagttg acccgagcgc
     1081 gctgtgaggt tttgcggccc tgggcggcgg agcgtcacgt ggagctccgc gtcgatggcg
     1141 acgcaagcgt cagcgcgcac gcggactctc tcgctcgcgc tctcgacaac ctgatccgca
     1201 acgcggtcga ggcgtcgagc cccgaaagta cggtagcgat tcgaatcagc gaagacgagg
     1261 gacgagcgaa ggtcagcgtg cgcgatcggg gtcctggcgt cgaagcccag cgcgcgagcg
     1321 agctcttcga gccgttcttc acgaccaagc ccgacggcac ggggctcggg ttaccgctcg
     1381 ctcgttccat agctcaggcg cacggcggcg acgtgcgtta tgtgcgggtc gatgccgaaa
     1441 cgcgcttcga agtcgagttg ccgctcgcta gcgagaatgg cgcgcctcca agatcccccg
     1501 aggccgagag cgagctacgg ttccattcat gaaggaccag cctcagagcg aagcgccggc
     1561 ccgggtgctc atcgtcgagg atgagcgtgg gttacgcgag ggcttgatgg gcgcagtcga
     1621 gcgcatcggg tgcgaggcgc tcgcggccgc cggcgtgcag gaggcgcggc agcgactcgc
     1681 ggagcacgag gtcgattgcg ttttgctcga tatacggttg aaggacggcg acggtctcga
     1741 gctcctgacc gagctcaggg ccgggccgtt gcgagacacg ccggtcatcg tcgcgaccgc
     1801 gtacagcgac agcgagcgca ccattcgggc gatgcgtgac ggcgcgttcg actacgtcac
     1861 caagcccttc gatcttccgg cactcctcgt cacggtgcga cgcgcgctcg accagcgccg
     1921 gttgcggcgt gccgtgaacg accccgccga gccgcctcgc gctctgccgc aaggtctcgt
     1981 cggcgcgagc gcggcgatgc tcgcgacctg gaagctcatc gggcgcgcgg ccgcgtcgtc
     2041 tgcgccggtg ttggtcaccg gagagaccgg cgtcggcaaa gagctcgtcg cgcgcgccgt
     2101 gcacgactac tcgacgcgcg tgacgcagcc gttcgtcgcc gtgaacctcg ccgcgctacc
     2161 gccgactctc atcgagagcg agctgttcgg gcacgaacgc ggcgctttca ccggcgcgag
     2221 cggtcgccgc gtcgggcggt tcgaactcgc cgccgggggc acgctgttct tggacgagat
     2281 tggcgatctc gatccttcgc ttcaaaccaa gctcctgcga gtgctccaag agggcacctt
     2341 cgagcgcgtc ggcggccaag agacgctcga gttcggtgcg cgcgtggtca ccgcgaccaa
     2401 caagccggtt caccccgggc agcccggctg tgcgctgcgc gaggatctct attaccggct
     2461 cgcggtcgtg gagattgagg tgcccccgct ccgggcgcgc aagagcgaca ttccgctcct
     2521 cgtggcgcac gcgctgcgaa gcacgcccgc gcgcgcggtc tccgaacagg cgatggagcg
     2581 cctgctcgcg tatgcgtggc ccgggaacgt gcgagagctc attcacgtca tcgagcgcgc
     2641 agcagtgctc tgcggaggcg agatcatcga cgttgcggat ctgcccgacg cggtgcgcga
     2701 gcccgtggcc agagcctctg cccccgaacc gctcgagact tcgaacctgc gcgaagcagt
     2761 ggctcgtctc gagcggcgcc tcatcgcggc tgccctcgtc gaaactcagg gtaaccgctc
     2821 cgaagctgcg cgccgcctcg gcatcgcgcg cgcgcagctc tacgcgaaga tcgaagagta
     2881 cggactgtag ctgctcacga gcgctgctcg gtgatctgct cgcgaagccg ttgctcctga
     2941 gcgatgagct cgggagtgag cgcttcgccg aagtccgacg cctcgaacac gggacgaatt
     3001 tcgaccgcgc actccggctc gcccggaccg gggctcggga tc
//
LOCUS       DASTKS010000252         8675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_262040, whole genome shotgun sequence.
ACCESSION   DASTKS010000252 DASTKS010000000
VERSION     DASTKS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262040"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..778)
                     /locus_tag="VFQ35_05230"
     CDS             complement(233..778)
                     /locus_tag="VFQ35_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9930061.1"
                     /translation="MDVEECVDDEASLGVLFARHAPSLRRMLSRRRGSAPVGTRALDK
                     DLDDLVPDTFLSVLLCTRRVPSLRVTPAYLFTVARNLCIDRWWRRGKSVCVELGGSDA
                     AAVFPEEFESECDRRERAEALARYVEQLPPSLLSIYEARFVRGLSQRDAAADLGISRG
                     QLRSLEQCLFSGAMSELVRTT"
     gene            complement(1027..2835)
                     /locus_tag="VFQ35_05235"
     CDS             complement(1027..2835)
                     /locus_tag="VFQ35_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930062.1"
                     /translation="MLSRRIRNDAVHALFAQSAPVLASNLVVAAVVAFTLRHAVPHVL
                     LLSWMGAMTAMVAVRIESIRRYWRAPAADGALWGMRFVWGTASAGVLWGCAAALWFDA
                     EPPFSEFVLTFAIGGMCAAAAGTLSVHLPAFFVFFLLALSPLVIRNVMVGDSAHVAAA
                     GMLMAFGVFLQGVARNNQRSFSKAWMAAHENAELLERLSQAQRALEETNRELERKVEA
                     RTLELERHAEALHHARHLEALGRLAGGLAHDFNNLMTVILTHTSELKQRAQRKGDEDE
                     SLLDEMRGAALHGAALVNGLLAFSRQRRIEPRQIDLDALVTQMMPWLRRVAGESVQIV
                     AESSRARMLVFADPAGLEELITNLCVNARNAMPRGGTMRLGLGVSVHSLPGAREPQEC
                     AVLSVSDSGDGMSEQTRRSIFEPFAGARGLGEPGLGLATAYGIVRQNGGDIVVASELG
                     RGSEFRVFLPLSEASAEASRPRASTGPVRSLTILVAEDEPSVRAAVARCFRSAGHTVM
                     VAEDGRHALEIAERYAGTIDVLVTDVVMPNLGGPELAERLARERPGVRVLFTTGHSFG
                     ATLPADDPTRGVAVLSKPFDNHALESKLRSLFGNTP"
     gene            3467..4588
                     /locus_tag="VFQ35_05240"
     CDS             3467..4588
                     /locus_tag="VFQ35_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930063.1"
                     /translation="MRLGAISLVAFASAIVFACVDGRTYDDPNQQGGSTGTGGHSQGG
                     AASGGATSGGVPGSGGSSVSGGGPTASGGSSGSGGSDTGGGSATGGSSSGGSAGNSSG
                     GKSSGGSAGSAGNGGNGNRGGGSNSGGMTGAGGMQGQGGAMVTEPPRLSSGSNGWASR
                     YWDCCKPACGWSGNVKSGQPMNSCDKSNNVLGDKNAKNACENGGSAFMCWSGVPWAVS
                     NTLALGFAAASGGNYVCGRCYQIQFTGQGHNGSPGSPQLNNKTMIVQVINNGGVQADQ
                     FDILIPGGGVGALNACSNQWGTSDLGAQYGGFLAGCNGDKNCVKQKCQSVFNGKPDLL
                     EGCDWFLNWYGGADNPSFIFKQIACPAAITQKSGLSDPG"
     gene            4685..5062
                     /locus_tag="VFQ35_05245"
     CDS             4685..5062
                     /locus_tag="VFQ35_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930064.1"
                     /translation="MADDDAPRDASGTGGADVALIHGVTDNGDLKIVRQREGRLELGE
                     VRPLREGVPIAGEVVKLTPRKEFPLLCDVTSELAVAPSAPKQDVVEAPVGHKGPAQVA
                     TDRYRENWDLIWKRRSSGNERAN"
     gene            complement(5068..7227)
                     /locus_tag="VFQ35_05250"
     CDS             complement(5068..7227)
                     /locus_tag="VFQ35_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET9930065.1"
                     /translation="MKLERSVIVNSLVLALALGSGVAVLVTRKFATTDEQSARKNNLC
                     AELPREDVKRLRLREGANAFVLVRKAAVDGGAPEFELEGQGPADAEAAESLLRALEVA
                     SFDRRFDDKEVDLHAFGLDTPRAELHLELAASELEIEVGKAAATGAESYVRTRSRSHG
                     VTVGLVKNATLKDLFPSADDLRPRTILPLAVSELREVAFKDAGHDVHVHRGKGPSFLD
                     ANNRRVARDAVERLGYELFGLKAERFVSLPEANAALDAGGSLDASFTRNDGVSFAARF
                     GGTCPGAPDLSVFVRSKPTALAACITPGARQLFWNTANNLENEQTFDLHVDEVESVRI
                     EQGDKKLELVRNERGFSLRAPSVATVALDAGNDRIRALIAEGTRVLEPDSSALGLGFP
                     GGKVTLHSTSLHEPPSFDEVLELGRVQRDGRLPVRRAEDGVVLLLSRDVARAFQVDST
                     LLRARELFDFGASDFVELDVSFGREREKLRRSPAGVFELASPPAPHDATLSLELVQAL
                     GALSVQRWVADDDDGSFGLESPRVRAELTLTPNDGGARHFTLLIGKDTAGGAYAKLAE
                     RAGVFVLERDIVTQLTTLLLTRAAFASDPNSVERVELEHRGNRRVLTRSGGALRVVEG
                     STLPPDVIATISEVLANLRPEAALHSGAATADEGFEKPLLRAKVVTRAGLGVDKTFRI
                     GAGDVYRDTPVYYARVDGIDATYVIAKSALRPLLDAL"
     gene            complement(7211..>8675)
                     /locus_tag="VFQ35_05255"
     CDS             complement(7211..>8675)
                     /locus_tag="VFQ35_05255"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET9930066.1"
                     /translation="NANLLVARFYKRWDLTTDQLYTLSDATRGTLHDLKQPVRVLVLL
                     SRGDPLLSSVRELLVAYRAETTELELEFVDPAQNPAEFLAIQQKYDIQAGRSDDGRVL
                     TDAALIVAQGERHWFVTADELVSFDADSGSVRPRIERALTAGIVNVLGLEKAKVCFTR
                     GHQELSLNDAGPEGLIELRNRIEKSNYIAEERDLPGTSSTPLEGCRLTVVAGPRLPFE
                     AKDSAALTQALREGMSALVLSSPQLGESGTIKSTGLEPVAEEAGAELGRNLVIESDTS
                     ARLPRGIGEVFFAVPKEHAVTAGLLKGGAKIEARVLVSEASSLRLRSPEAKALLASSR
                     EAFVIDDVRPLLDGRLDSSLAPHGEQTLAVARELPKPASAKHPPRIVFVTAANVAQNR
                     SFRDSGLFGDRIFLENAVSWLAARPALVSVPDKAPQEVGLALSEESLGEVMRYVLIYM
                     PGSAALLGLAVILRRRAVEKRSRKDAESRRGDDEARA"
BASE COUNT         1492 a         2873 c         2888 g         1422 t
ORIGIN      
        1 ccgacgttca gaacatcggt aacggaaacg attgggtcgc cgtcacgcag gccgtcggct
       61 ccgatgacaa gatccgaccg gtcgaagtct tgctctacag ctactgagtt ggcgagctgc
      121 agcgcacgct cattcgagcg cggcgcgcgg aggacgcccg gcggtcatcg tgggccgccg
      181 ggcgctggct cgtgaatcga gacgtgggtc cgagcggccg agatcgccct ccctaagtgg
      241 tgcgaacgag ttcgctcatg gctccgctga acaagcactg ctcgaggcta cgaagctgtc
      301 cgcgcgaaat gcccaagtcg gccgcggcgt cgcgctgcga gagaccgcgc acgaagcgcg
      361 cttcgtagat cgaaagaaga ctcggcggca actgctctac gtagcgagca agcgcttcgg
      421 cgcgctctcg ccggtcgcat tccgactcga actcttcagg gaaaaccgcc gcggcgtcgc
      481 tcccgccgag ctcgacgcat acgcttttcc cacggcgcca ccagcgatcg atacagaggt
      541 tacgcgccac ggtgaatagg tacgccggcg tcacacgcag cgatggaacg cgtcgggtgc
      601 aaaggagcac gctcaagaac gtgtccggca ctagatcgtc caggtccttg tccaaagccc
      661 gagtgccaac gggagcgctc ccgcggcgtc gactcagcat tcgccgcagg gaaggcgcgt
      721 gccgtgcaaa caacacccct agagacgctt catcgtccac gcattcttcg acgtccatag
      781 cgtgatctct ccccggggcg tgtgcacacg acgctcgcta cgacaccccc caccttccga
      841 gacatgctac gtcgaacgac cccgatcact cttgtacgcc gttgagagtg ccaacgcacc
      901 gcctgcacga aaatacaact cgcactgacc tcatcagcaa ggtggcgtgc cgagtcaaag
      961 gcgcctaggg gctgcgcgcg tctgtcggga ccgcgtcggc ccgtagcgcg ctacgacgga
     1021 atgcgcctag ggagtgttcc cgaacagcga gcgcagcttg ctctcgagcg cgtgattgtc
     1081 gaacggcttc gagagaacgg cgactccgcg cgtcgggtcg tccgcgggca gcgtcgcgcc
     1141 gaagctgtgg cccgtcgtga agaggacgcg cacgccgggg cgttcgcgag cgagtctttc
     1201 cgcgagctcc ggcccgccca ggttgggcat gaccacatcg gtgacgagga cgtcgatggt
     1261 gccagcgtag cgctcggcta tctccagggc gtggcggcca tcctcggcaa ccatcacggt
     1321 gtgtccggcc gagcgaaagc agcgagcgac tgccgcgcgc acgctgggct cgtcttccgc
     1381 gaccaggatg gtgagtgagc gaacgggccc cgtgctcgcg cgtgggcgcg aagcctcggc
     1441 gctcgcctcc gacaacggaa ggaagacgcg gaactcgctg cctcgaccga gctccgaggc
     1501 gaccacgatg tcgccgccgt tctgtcgcac gatgccgtag gcggtagcga gaccgagccc
     1561 gggctcgcca agaccgcggg caccggcgaa cggctcgaag atgctgcgcc gagtttgctc
     1621 gctcatgccg tcgccgctgt cgctcacgga caggacggcg cactcttgcg gctcgcgcgc
     1681 gccgggcaac gaatgcaccg aaacacccaa gccgagccgc atcgttcctc ctcgcggcat
     1741 cgcgttacgc gcgttgacac agaggttcgt gatgagctcc tcgagccccg ctgggtcggc
     1801 gaagacgagc atacgcgccc gcgacgactc cgcgactatc tggacgctct cgccagcgac
     1861 tcgtctgagc cacggcatca tttgagtaac gagcgcgtcg aggtctatct gacgaggttc
     1921 gatgcgccgc tggcggctga aggcgagcag cccgttcacg agcgcagcgc cgtgcagcgc
     1981 agcgccgcgc atctcgtcga gcaagctttc gtcctcgtcg ccctttcgct gcgcgcgctg
     2041 cttcaactcg ctcgtgtgag tcaagatgac ggtcatcaag ttgttgaagt cgtgcgcgag
     2101 cccgcccgcc aagcgcccga gcgcctcgag gtgccgcgcg tgatgcagcg cctctgcgtg
     2161 tcgctcgagc tccagcgttc gggcttcgac cttgcgctcg agctcgcggt tggtttcttc
     2221 gagcgcgcgc tgggcctgcg aaagtcgctc gagtagctcc gcgttttcgt gagcggccat
     2281 ccacgctttc gaaaacgagc gctggttgtt gcgcgcgaca ccttgcaaga agacgccgaa
     2341 ggccatcagc attccagccg ccgcgacgtg agcggagtcg ccgaccatta cgtttcggat
     2401 cacgagcggc gaaagcgcca agaggaagaa cacgaagaac gccggcaggt gcaccgagag
     2461 cgttcccgcc gccgctgcgc acatccctcc aatcgcgaag gtgagcacga actccgaaaa
     2521 cggtggctct gcgtcgaacc acagcgccgc cgcgcagccc caaagcacgc ccgcggaggc
     2581 cgtgccccaa acgaagcgca ttccccagag cgctccgtct gctgccggtg cgcgccaata
     2641 tcggcggatc gactcgatcc gaacggcgac catcgcagtc atcgcgccca tccacgagag
     2701 cagcagaacg tgcgggacag cgtggcgcag agtgaacgca acgacggctg ccacgaccag
     2761 gttgctcgcc aacaccggtg cgctctgcgc aaacagcgcg tggacggcgt cgttgcgaat
     2821 gcgccgtgac agcacgctcc cactataagt ccggcgtgaa actcccgccg ctaaatcgac
     2881 ggttccgagt tcgcgatcag gcacgacctg acgagtgtca ggatagtaac ttcctcgaga
     2941 tgcgagagcg cgttcgcgat cgagctcgcg cgaagacctc gtgacttcgg ggctcagcgg
     3001 ccgggacacg cactcgcgaa cctctcccgg ctcgcgaagt cgtctcacga aacagcccta
     3061 ttgggcgctt cggttgcggg cgccgagtcc gaggcggaac caggccgaaa ccggtggttc
     3121 gtcggctggt ctgcgatggc ggcagaaagc ccgtcccgcc tgcttcgaac gcggggcgac
     3181 cggccgaagc tcctcggtcg ctcgcgctct tggcccctcg gaagcgaaac gcggagcgct
     3241 gccgtcccat ctcgaagcga gacgcgaatt ctggtaatcc cgaacatata cacccagcac
     3301 gcggcgcaga cccagctcac tcaaatgtta acagcgttaa cttaatgatg tgaagcgcgc
     3361 cggcacggcc tagtttcgct gattttgtgg gagtcggcgc actcatgcca tcgctcccgg
     3421 ctgcgttcct tgcaaaacgg gcgtctccct ctatacgtgt gggccaatga gactcggcgc
     3481 catctcgctc gtcgcttttg cgtccgcgat cgtcttcgct tgcgtcgacg gtagaaccta
     3541 cgacgacccg aaccagcaag gcgggagcac cggaactggt ggtcactccc agggcggcgc
     3601 cgcgtccggc ggggcaactt ccggcggcgt gccgggctcc ggtggctcgt cggtgagcgg
     3661 cggcggcccg actgcgagcg gagggtctag cgggagcgga ggatcggata ccggaggcgg
     3721 cagcgcgacc ggtggaagca gcagcggcgg ctcggcaggg aactcttctg gcggaaagag
     3781 ctccggcggt tctgcgggct cggctggaaa cggcggcaat ggcaaccgcg gcggcggctc
     3841 caattccggc gggatgaccg gcgcgggcgg catgcagggg cagggcggag ccatggtgac
     3901 cgagcctcca cgcctctcga gtggaagcaa cggctgggct tcgcgctact gggattgctg
     3961 caagcccgcg tgcggttggt cgggcaacgt caagagcggg cagccgatga actcgtgcga
     4021 caagtcgaac aacgtgctcg gcgacaagaa cgccaagaac gcgtgcgaga acggcggaag
     4081 cgccttcatg tgctggagcg gcgttccctg ggcagtgagc aacactttgg cgctcggctt
     4141 cgctgcggcg tcgggcggca actacgtgtg cggccgttgc taccaaattc aattcaccgg
     4201 tcagggccac aacggcagcc ccggctcgcc ccagctcaac aacaagacga tgatcgtgca
     4261 ggtcatcaac aacggcggcg tgcaggccga tcagttcgac atcttgatcc caggcggcgg
     4321 cgtgggcgcg ctgaacgcgt gctcgaatca gtggggaacg tccgacctcg gcgcccaata
     4381 cggcgggttc ctcgccggct gcaacggcga caagaactgc gtgaagcaga agtgccagag
     4441 tgtcttcaac ggcaagccag acctgctcga gggttgcgat tggttcttga attggtacgg
     4501 cggcgccgac aatccgagct tcatcttcaa gcagatcgcc tgccccgcgg cgatcacgca
     4561 gaagtcgggc ctgtcggacc ccggctgagc gccaattttt agggattttt gccgagccga
     4621 cgagcctcgg gcgcggccgc gtgacgtgcg aaaaccgccg tttcgtgtta ttcgttacgg
     4681 caacatggca gacgacgacg ctcctcgcga cgcctcgggc acggggggag ccgacgtcgc
     4741 gttgatccat ggagtcacgg acaacggcga cttgaagatc gtgcgccagc gcgagggccg
     4801 cttggagctc ggcgaggtgc gtcccctgcg cgagggggtg ccgatcgccg gggaagtcgt
     4861 gaagctcact ccgcgcaagg aattcccgct cctttgcgac gtcacctccg agctagcggt
     4921 cgcaccctcc gcgccgaagc aagacgtcgt cgaggcgccc gtcgggcaca aggggcccgc
     4981 ccaggtcgct accgatcgct accgagagaa ctgggatctg atctggaagc gccgctcttc
     5041 cggtaacgag cgcgccaact agggttctca gagcgcgtcg agcagcggtc gtagcgcgct
     5101 cttcgcgatg acgtacgtcg cgtcgatgcc gtccacgcgc gcgtagtaaa ccggcgtgtc
     5161 tcggtagacg tcgcctgcgc cgatgcggaa tgtcttgtcc actccgagcc cggcgcgcgt
     5221 gacgactttg gctcgcagca gcggcttctc gaagccctcg tcagccgtcg cggcaccgct
     5281 gtggagcgca gcctcgggcc tcaggttggc gagcacctcg gagatggtcg cgatcacgtc
     5341 gggtggtaag gtcgatccct cgacgacgcg cagcgcgccg ccgctgcgag tgaggacgcg
     5401 ccggttgccg cggtgctcga gctcgactcg ctccacgctg ttcgggtcgc tcgcgaacgc
     5461 ggcgcgggtg agcagcaacg tggtgagctg cgtgacgata tcgcgctcca gaacgaagac
     5521 gcccgcgcgt tcggcgagct tcgcgtaggc gccgccagcg gtgtccttgc cgatcaacaa
     5581 ggtgaaatgg cgggcgcccc cgtcgttcgg agtgagagtc aactccgcgc ggacacgcgg
     5641 cgattcgagg ccgaagctgc cgtcgtcgtc gtctgcgacc cagcgctgca cactgagcgc
     5701 gccgagcgcc tggacgagct cgaggctcag cgtcgcgtcg tgcggcgccg ggggtgaagc
     5761 gagctcgaac accccagctg gcgagcgccg cagcttctcc cgctcgcggc cgaagctgac
     5821 gtcgagctcc acgaagtccg atgcgccgaa atcgaatagc tcgcgcgccc tgagcagcgt
     5881 cgagtccacc tgaaaggcgc gcgcgacatc gcggctcaag agcaggacga cgccatcctc
     5941 ggcgcgccgc accgggagcc tgccatctcg ctgcacgcga ccgagctcga gcacttcatc
     6001 gaagctcggc ggctcgtgga gcgaggtgct gtgcaaggtc acctttcccc ccggaaagcc
     6061 gagtccgagc gcgctcgagt cgggctcgag gacgcgagtt ccctccgcga tcagcgctcg
     6121 gattcgatcg ttcccagcgt cgagagcgac ggtggctacg ctcggcgcgc gaagcgagaa
     6181 gccccgctcg ttgcgcacga gctcgagctt cttgtcgcct tgttcgatgc gcacgctctc
     6241 gacctcgtcg acgtgcaaat cgaaggtctg ctcgttctcg aggttgttgg ccgtgttcca
     6301 gaacagctgg cgtgctcccg gagtgatgca cgcggcgaga gccgtcggct tgctgcgcac
     6361 gaaaacgctg aggtccgggg cgccagggca agttccgccg aagcgcgcgg cgaagctcac
     6421 gccgtcgttg cgagtgaagc ttgcgtcgag cgaccctccc gcgtcgaggg cggcgttcgc
     6481 ctccggtaag ctcacgaagc gctctgcttt caacccgaag agctcgtagc cgaggcgctc
     6541 caccgcgtcg cgcgccacgc gacggttgtt cgcgtcgagg aaggaggggc ccttgccccg
     6601 gtgcacgtgc acgtcatggc ccgcgtcttt gaaggcgacc tcgcgcagct ccgacacggc
     6661 gagcggcaag atggttcggg gccgaaggtc gtcggcgctc ggaaataggt ccttgagagt
     6721 cgcgttcttc accaagccaa cggttacgcc atggctccgc gagcgcgtcc ggacgtagga
     6781 ctcggcgccg gtcgcagcgg ccttaccgac ctcgatctcg agctccgacg ccgcgagctc
     6841 gaggtgtaac tcggcgcgcg gcgtatcgag cccgaaggcg tgcaagtcga cctctttgtc
     6901 gtcgaagcgt cggtcgaagc tcgcgacttc gagtgcgcgc aggagcgatt cggcggcctc
     6961 cgcgtcggcg ggcccctgac cttcgagctc gaactcgggc gcccccccgt cgaccgccgc
     7021 tttgcgaacc aagacgaagg cgttcgctcc ttcacgcaag cgcaggcgtt tgacgtcttc
     7081 gcgggggagc tcggcgcaca ggttgttttt gcgcgcgctc tgctcgtcgg tggtggcgaa
     7141 ctttcgagta acgaggaccg ccacgccgct cccgagggcc agcgcgagca cgaggctgtt
     7201 cacgatcacg ctacgctcga gcttcatcgt ctcctcggcg cgactcggcg tctttgcgcg
     7261 agcgcttctc caccgcacgt ctccgcaaaa tcacggcgag cccgagcagc gccgccgagc
     7321 ctggcatgta gatgagcacg tagcgcatga cctcgccgag cgattcttcg ctgagcgcga
     7381 gcccgacctc ctggggcgcc ttgtcgggca cgctcactag cgccggccgc gctgcgagcc
     7441 acgaaacggc gttctcgagg aagatgcgat cgccgaacag cccagagtcg cggaagctgc
     7501 ggttttgcgc cacgttcgcc gccgtgacga agacgatgcg cggcgggtgc ttggccgaag
     7561 cgggcttggg gagctcgcgc gcgacggcca acgtctgctc tccgtgcgga gcgagcgacg
     7621 aatccagccg gccgtcgagc agcgggcgca cgtcgtcgat gacgaaggct tcgcggctcg
     7681 aggcgaggag cgccttcgct tccgggcttc gaaggcggag gctcgacgct tcgctcacga
     7741 ggacgcgcgc ctcgattttg gcgccgcctt tcaacagccc agccgtgacc gcgtgctctt
     7801 tgggcactgc gaagaatacc tcgccgatgc cgcgaggcag gcgcgccgag gtgtcgcttt
     7861 cgatgaccaa gttgcgaccc agctccgcgc ccgcctcctc cgcgactggc tctagcccgg
     7921 tactcttgat cgtccccgac tcgccgagct gcgggctcga gagtacgagc gcgctcatgc
     7981 cttcgcgcag agcttgggtc aacgcggcgc tgtctttcgc ctcgaagggc aagcgaggac
     8041 cggcgacgac agtcagacgg cagccctcga gcggcgtgct cgatgtacct ggcagatcgc
     8101 gctcctcggc gatgtaattg ctcttttcga tgcgattgcg aagctcgatg agcccttcgg
     8161 gtccagcgtc gttgaggctc agctcttgat gtccgcgcgt gaagcaaacc ttcgcttttt
     8221 cgaggccgag cacgttgacg atgccagccg tgagcgcgcg ctcgatgcgc ggtcgtacac
     8281 tgcccgaatc ggcgtcgaag ctcacgagct cgtcggcggt gacgaaccag tggcgctcgc
     8341 cctgagccac gatcaacgcc gcgtcggtca gtacgcggcc gtcatcggag cggcctgctt
     8401 ggatgtcgta cttttgctga atcgccagga actcggcagg gttctgcgct ggatcgacga
     8461 actcgagttc gagctcggtc gtctccgctc ggtaagccac gaggagctcc ctcacactgc
     8521 tgagcagcgg gtcgccgcgg ctcaaaagca cgaggactcg caccggctgc ttcaggtcgt
     8581 gcagggtgcc acgcgtcgca tcggagagcg tatagagctg gtccgttgtg aggtcccagc
     8641 gcttgtaaaa gcgtgccacc agcaagttcg cgttc
//
LOCUS       DASTKS010000253         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_932106, whole genome shotgun sequence.
ACCESSION   DASTKS010000253 DASTKS010000000
VERSION     DASTKS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932106"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFQ35_05260"
     CDS             <1..314
                     /locus_tag="VFQ35_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9930067.1"
                     /translation="THVDVLARFGARATHVRCRLETGRTHQIRVHLAEAGTPILGDPL
                     YGKPPRDPMLREIAAALGHQALCARVLGFVHPRTGKTMRFEVPLPAEFQAALRALADS
                     E"
     gene            399..1388
                     /locus_tag="VFQ35_05265"
     CDS             399..1388
                     /locus_tag="VFQ35_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9930068.1"
                     /translation="MGRRGALETVTAVLVAFLEERTWEQAALARRVGVTTRTLRSCLL
                     GLAESGVPLEADAENGSRVFWSVPAKWLPGAASLTEDEVSLCARILARTPRTTGRDRL
                     LGRLLPGAGASSARNDLDARARESVLSAIEDALRDAAAVRASYRSTAHGVARERTISI
                     QRIAYGEPVRVVGHCHRTNELRWFRVDRFESVSRATSEPFVRVDATLVHGFIRESLDG
                     FHGSASAAEVSFVLKDPSARWAIANLPPMDAVVSSVPDEGWRVVVRTSGLDVLARWLV
                     GLGAAARVETPELRDRVARLAEDALAVNGGPRLAKARARPRDAEGQRRVAPKG"
     gene            1466..>2394
                     /locus_tag="VFQ35_05270"
     CDS             1466..>2394
                     /locus_tag="VFQ35_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9930069.1"
                     /translation="MTSSFERWFEDVTAFAPHAWQLALGTEHACSDRLLRIPTGFGKT
                     AGAVLSWAYHRVVRNDATWPTRLVFCLPMRVLVEQTERSIEAWFEKAKLDVPVIVLLG
                     GRAEEGWLEHPERPAVVIGTQDMLLSRALTRGYGSTRGLWPMEMGMVHGDALWVIDEV
                     QLMDVGLATSAQLHAFRVAEAKRTAAIRPTFTWWMSATLQPSWLETIDFNAVRDTRPR
                     PAMRIESAQRHGGLWDVKKRLERRADVAAPEEVAALVRERHRPGSMTLVVVNTVDRAK
                     RVDAAVSKLKVDAELRLVHSRFRAKERALWDFLR"
BASE COUNT          366 a          764 c          865 g          399 t
ORIGIN      
        1 tgacgcacgt cgacgtgctc gcgcgcttcg gcgcgcgcgc gacccacgtg cgctgccgac
       61 tcgagaccgg acgcacgcac cagatccgcg tccacctcgc cgaggcgggc acgcccatcc
      121 tcggcgatcc gctctacgga aagccgccgc gcgatccgat gctgagagag atcgccgccg
      181 cgctcgggca ccaggcgctc tgcgcccgcg tgctcggctt cgttcatccg cgaacgggga
      241 agacgatgcg cttcgaggtg ccgctgcccg cggagtttca agcggcgctg cgcgcgctcg
      301 ccgacagtga gtagcgagtc gggggcacgg gcgcttgtcc aaaatttgga cgcgttctgt
      361 cggcgacaac gcgcgcgatc atcccgtacg ttgcgtgaat ggggaggcgt ggggcgctgg
      421 agacggtgac cgcggtgttg gtcgcgttct tggaggaacg cacgtgggag caagcggcgc
      481 tcgcgcggcg cgtcggtgtc acgacgagga cgctccgaag ctgtttgctg ggtttggccg
      541 agagcggagt gccgctcgaa gccgacgccg agaacggctc gcgcgtgttt tggagcgtgc
      601 cggcgaaatg gcttccgggt gcagcctcgc tcacggagga cgaagtctcg ctatgcgcgc
      661 gcatcctcgc gcggacgccg aggaccaccg gtcgtgaccg gctgctcggc cggttgcttc
      721 caggtgctgg cgcgagctcc gctcgcaacg atctggatgc gcgggcgcgc gaatcggtgc
      781 tgagcgcgat cgaggacgcg cttcgtgacg cggccgcggt tcgtgcaagc taccgctcga
      841 cggctcacgg cgtggcgcgc gagcggacga tatccattca gcgcatcgcg tatggtgagc
      901 cagtgcgcgt ggtcgggcat tgccaccgta cgaacgagct gcgctggttt cgcgtcgatc
      961 ggttcgaaag cgtctcacgc gcgacgagtg agccgttcgt acgagtcgac gccactctcg
     1021 tgcatggctt cattcgcgag agcctcgacg gctttcacgg gagcgcgagc gccgcggagg
     1081 tctcattcgt tctcaaggat ccgagcgcgc gctgggcgat cgcgaatctg cccccgatgg
     1141 acgcggtcgt ctcctcggtt cccgacgagg gctggcgcgt cgtcgtgcgc acatccggcc
     1201 tcgatgtcct cgcccgatgg ctggtcggcc tgggcgcggc ggcacgggtc gaaactccag
     1261 aacttcgcga ccgcgtcgcg cgactcgccg aggacgcact tgcggtgaat ggcgggccac
     1321 ggttagcgaa ggcacgcgcg cgtccgcgcg atgcagaagg ccaacgacgc gtggcgccga
     1381 agggctgacg aggcggccac cgggtagacg gattagcgca ccgatgcgtg cttgagtgtc
     1441 gagcaaccag gggaggggcg gtcaggtgac gagttcgttt gagcgatggt tcgaggacgt
     1501 gacggcgttt gcgccgcacg cttggcagct cgcgctcggc accgagcacg cgtgcagcga
     1561 ccgccttctg aggattccaa ccggcttcgg caagaccgca ggagccgtgc tctcgtgggc
     1621 gtatcaccgc gtcgttcgaa acgacgcgac ctggccgacg cggctcgtct tctgcctccc
     1681 catgcgggtg ctcgtcgaac agacggagcg atcgatcgag gcgtggttcg agaaggcgaa
     1741 gctcgacgtg ccggtcatcg tcttgctcgg cggtcgggcc gaggaaggtt ggctcgagca
     1801 ccccgagcgt cctgccgtcg tcatcgggac gcaggacatg ttgctctccc gcgcgctcac
     1861 gcgtggctac ggctcgacgc gtgggctctg gcccatggag atgggcatgg tgcacggcga
     1921 cgccctctgg gtcatcgacg aggtgcagct catggatgta gggctcgcaa cgagcgcgca
     1981 gctccacgcg ttccgcgtcg ccgaggcgaa gcgcacggcg gcgattcgac ccacgttcac
     2041 ctggtggatg agcgccacgc tccagccatc gtggctggag accatcgact tcaacgccgt
     2101 gcgcgacact cgtccgcgac cggccatgcg catcgagtca gcgcaacgac acggcgggct
     2161 ttgggacgtc aaaaagcggc tcgaaaggcg tgccgacgtt gcggcccccg aagaagtcgc
     2221 ggcgctcgtg cgtgagcgtc atcggccggg ttcgatgacg ctcgtcgtcg tcaacaccgt
     2281 ggatcgcgcg aagcgagtgg atgcggcggt gtcgaagctc aaagtcgatg ccgagcttcg
     2341 actggttcac tcgcgcttcc gggccaagga gcgcgctctg tgggatttct tgcg
//
LOCUS       DASTKS010000254         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_487199, whole genome shotgun sequence.
ACCESSION   DASTKS010000254 DASTKS010000000
VERSION     DASTKS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487199"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFQ35_05275"
     CDS             complement(<1..195)
                     /locus_tag="VFQ35_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930070.1"
                     /translation="MSRSLPQRNAGDRRYKLRGQFEYGGLPDVHLAFLEGAGGFRRTV
                     VVQRLSSAASSTRGGEPLLSP"
     gene            462..1343
                     /locus_tag="VFQ35_05280"
     CDS             462..1343
                     /locus_tag="VFQ35_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930071.1"
                     /translation="MRAALVLLCGAWVLAPVDARALDKQGSAHGGKVDYDDHGSALTG
                     SLLFGVAIYNPSYAARPDNSGHALLRVAPHFDFDIIGRHLSIPIDVNVFTDRDRVGVK
                     MFLPSELDVITGVTSTWPLGEPTAIEFGARFERDMPVDRQTYTQSYADLRTRLLYSFA
                     PIWRGLEQALDGGNLSGSCTLGWFAYNPSYAARPDNSGRALLRYGLGVNLDALHHHLG
                     VGVDLTSFTDRRTNGVEPTELDLTPSVSYRLGRTQLQLAYERDMPIDRTHLVQQLVMA
                     AATFDFRVFPGEPAEVK"
     gene            complement(1454..2038)
                     /locus_tag="VFQ35_05285"
     CDS             complement(1454..2038)
                     /locus_tag="VFQ35_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930072.1"
                     /translation="MARFRETLSLLFFLSACHPRSSAPTAQSDHGGKRQPAPAASQIK
                     PERGVMAAVAGVPPDYAEVAKELPLFEAAGLTREKAARFVLTLKDALARDDRDALAGL
                     VAYPMTVRVNGKTRVVKDRAELLRDYDQLFTPKVREAVLAQHVEHLKATGRGAEIGRG
                     ELRFRCPARLLTKSPGGAVTCDGPNIRIVHVDSD"
     gene            2112..>3297
                     /locus_tag="VFQ35_05290"
     CDS             2112..>3297
                     /locus_tag="VFQ35_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9930073.1"
                     /translation="MPPEATRSVALLGPTNTGKTHKAVERMLEHPNGMIGLPLRLLAR
                     EIYDRISARLGEDRVALVTGEEKRVPARPDYWVCTTEAMPLTREVDFIAVDEVQLATH
                     DERGHIFTDRLLHARGRRETWFMGAATLSGVVERLVPAAQRREHPRLSKLGFAGSSKL
                     SRLRPRSAIVAFSLKELYAIAGRLCSLRGGVALVMGALSPRTRNAQVALFQSGEVDYL
                     VATDAVGMGLNLDVDHVAFASLRKFDGQRARSLDAAEIAQIAGRAGRFLRDGTFGTVL
                     PLELERDVAQRVEGHRFDSVTRVRYRNSELDFSSPTSLLDSLRAPPPLACLSSVPAAE
                     DLRSLEALLKDPAISKLVGDPMRVELLWQVCQIPDFRRILFEVHVDLLRLIFSELVKG
                     RLS"
BASE COUNT          544 a         1088 c         1061 g          604 t
ORIGIN      
        1 gggcgatagc aggggttcac ctccgcgcgt cgacgatgcc gccgatgaga ggcgttggac
       61 caccaccgtg cgccgaaagc cccccgctcc ttccaggaag gcgaggtgca cgtcgggtag
      121 ccctccgtat tcgaactggc cccgcagctt gtaacgacgg tctcctgcgt tgcgttgcgg
      181 tagcgagcgc gacacgactt cggttcgatt cggttcttca gcgctgccga agagaggaag
      241 ggcgcgttct tgagttgcca aagcttcacg acggggagga cgagctgcca ctttgcgagc
      301 catcgacggg gatgaatcgg tgcggtgcgc gaaaagatct ggcgcaccgc attcgtctag
      361 tgtcgactgg gcaccccttg gttccccgaa acaatccggc gcgttctttt gcacaacttg
      421 gcaagcaccg aggtaggacc tctcgcgaca gtcgcgcacg ggtgcgcgcc gctctcgtgc
      481 tcctttgcgg ggcctgggtg ttggcgccgg tcgacgcacg tgcgctcgac aagcagggga
      541 gcgcgcacgg cggcaaggta gactacgacg accatgggtc cgcgctcacg ggctcgctgc
      601 tcttcggagt ggcgatctac aatcccagct acgcggcgcg acccgacaac agtgggcacg
      661 cgctgcttcg cgtcgcgccc cacttcgact tcgacatcat cggccggcat ctgtcgatcc
      721 ccatcgacgt gaacgtgttc accgaccgcg atcgcgtggg cgtgaagatg ttcttgccga
      781 gcgagctcga cgtcattacc ggggtcacga gcacgtggcc gctcggcgaa ccgaccgcga
      841 tcgagttcgg cgcgcgcttc gaacgcgaca tgccggtgga tcgccaaacc tatacgcagt
      901 cgtacgccga tttgcgcacg cgcctgctct attcgttcgc gccgatttgg agaggactcg
      961 agcaagcctt ggacggcgga aacctgagcg ggtcgtgcac cctcggttgg tttgcttaca
     1021 acccgagcta cgccgcgcgc cccgacaaca gcggtcgcgc gctgttacgc tacgggctcg
     1081 gtgtgaacct cgacgccctc catcaccact tgggtgtcgg cgtggatctc acgagcttca
     1141 cggatcgacg cacgaacggc gtagaaccaa ccgagctcga cttgactccg tcggtttcgt
     1201 atcggctcgg ccgaacccag ctccagcttg cctacgagcg cgacatgccg atcgaccgaa
     1261 cccatctcgt gcagcaactc gtgatggcgg cggcgacctt cgattttcgc gtgtttcccg
     1321 gcgaaccggc tgaagtcaaa tagcaggacc gttcacgagc accggcgctc caccgctctc
     1381 ccgccgagtc accgccatcc tacctagaac cgcgatgagt tcggatgctc ggcgttcctg
     1441 accgtttgtc gccctagtcg ctgtccacgt gcacgatgcg aatgttcggt ccatcgcacg
     1501 tgacggcgcc gcctggtgac ttcgtgagaa gacgcgccgg gcagcggaag cgaagctcgc
     1561 cccggccgat ttcggcgccc cgcccagtgg ccttcaggtg ctccacgtgc tgcgccagca
     1621 ccgcctcccg caccttcggc gtgaagagct ggtcgtagtc gcgcaagagt tcggcgcggt
     1681 ctttgacgac gcgcgtcttc ccgttgacac gcaccgtcat cgggtacgcg accaagcccg
     1741 ccaaagcatc gcgatcgtcg cgggcgagcg catctttcag cgtcagcacg aagcgagccg
     1801 ctttctcgcg cgtgaggccg gcggcttcga acaaaggcag ctccttggca acctccgcgt
     1861 aatcgggtgg tacgccggcg accgcggcca tcactccccg ctctggcttg atttggctcg
     1921 cggcgggagc tggctgccgt ttcccgccgt ggtcgctctg cgcggtcggt gcgctcgacc
     1981 gcggatgaca agcggagagg aagaagagca gggagagggt ttcgcggaag cgggccaagg
     2041 attcggaaat atagcgcggc ggagcgctcc ccgcactggc tcaatataag cccgtcgggc
     2101 atgctcgtgg ggtgccgcca gaggccactc gcagcgttgc gctcctcggg ccaaccaaca
     2161 cgggtaagac ccacaaggcc gtcgagcgca tgctcgagca cccgaacggc atgatcggtc
     2221 tgcccctacg gctcctcgcc cgtgaaatct acgatcggat ttcagcgagg ttgggggagg
     2281 accgggtcgc gctggtgacc ggggaggaaa agcgcgtccc ggctcgcccg gactactggg
     2341 tgtgcactac cgaagcgatg ccgctcaccc gggaggtcga tttcatcgcc gtcgacgagg
     2401 tgcagctcgc gacacacgac gagcgagggc acatcttcac ggatcggctc ctgcacgcgc
     2461 gcggtcgccg tgagacctgg ttcatgggcg ccgcgacgct gtccggcgtc gtcgagcgct
     2521 tggtccccgc ggctcaacgg cgtgagcatc cgcgattgtc gaagttggga ttcgctggct
     2581 cgagcaagct ttcgcggctg cgccctcgga gcgccatcgt cgccttctcg ctgaaagagc
     2641 tctacgcaat cgcgggccgc ctgtgcagct tgcgtggggg cgtcgcgctc gtgatgggcg
     2701 ctttgtcgcc gcgcacacgc aatgcgcagg tcgcgctgtt tcagagcggc gaggtcgact
     2761 acctcgtggc gaccgatgcg gttggcatgg gcttgaacct ggacgtcgac catgtagcgt
     2821 tcgcgtcact tcgcaagttc gacggacagc gtgcgcgctc gctcgacgcc gccgagatcg
     2881 cgcagattgc cgggcgcgcg gggcgcttct tgcgcgacgg aacgttcggt acggtgttgc
     2941 ccctcgagct cgagcgcgac gtcgcgcaac gcgtcgaagg tcaccgcttc gactccgtga
     3001 cgcgggttcg ctatcgaaac tcggagctcg acttctcgtc gccgacgagc ctgctcgact
     3061 cactgcgagc cccgccacct ctcgcgtgcc tctcgagtgt ccccgccgca gaagacctgc
     3121 gttcgctcga agcgctcctg aaagatccgg cgatctcgaa gctcgtcggt gacccaatgc
     3181 gcgtcgagct tctctggcaa gtctgccaga tccccgattt tcgacgcatt ctattcgagg
     3241 tccacgtgga ccttttacgc ctcattttca gcgagctcgt gaaaggaagg ctgtcga
//
LOCUS       DASTKS010000255         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_41164,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000255 DASTKS010000000
VERSION     DASTKS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41164"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1589
                     /locus_tag="VFQ35_05295"
     CDS             255..1589
                     /locus_tag="VFQ35_05295"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET9930074.1"
                     /translation="MSREEAPHVFELDGHEVAVTHPSKPYFSRSAQVTKLELVQYYLS
                     VAEGALGGIRNRPIVLKRFVNGAEREAFYQKRAPEKRPSFIRTTTLSFPSGRTAEEVV
                     VDDRAGLAWVINLGCIELHPHAVRASDLEHPDELRVDLDPGPGVAWGDVRRVALEVNA
                     LLSELGLKGFPKTSGSRGMHVNVRIEPRWSFGEVRRAALALSREVERRLPQLATSKWW
                     KEERHGVFLDYNQNAKDRTTCSAYSVRPLPDARVSTPLEWSEVPDCEPADFTLFTVPR
                     RFAERGDPHAGIDAEAGSLEPLLELARRDEAAGLPDAPWPPHFRKMEGEAPRVAPSRA
                     RAKASSASEGEAKKTRTRSPRSKMPLLTVANSPDKAAAIAGLERWKAKYPEASTHLVA
                     EDVLVDAMRGRFSTWTRVRVNLRHVPEALRPPQETPDPDDDPTREWRERRAR"
     gene            complement(1596..2684)
                     /locus_tag="VFQ35_05300"
     CDS             complement(1596..2684)
                     /locus_tag="VFQ35_05300"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9930075.1"
                     /translation="MQLPINPPVLPMLAKRVSGLPEGEAFIFEPKWDGFRVLVFRDGD
                     EVTLQSRDKKPLNRYFPELLPVLLEQLPERCVLDGEVVVALGDRLDFGALQQRIHPAA
                     SRVKLLAQQTPASIVFFDLLCLGSEDLCSVPFEARRKRLEEVLRDARPPLHLTPATTD
                     RALASDWFQRFEGAGLDGVMAKPKTGRYEPDKRVMFKVKHERECDCVVAGFRWHKQGN
                     QTLVGSLLLGLYDDDGDLHHVGVCASFTQVRRRELVSELAPLRENALAKHPWRRWAEQ
                     GSAEGVGGEQRMPGGQSRWSQGKDLSWEPLRPELVVEVGYDHMEGSRFRHTAQFRRFR
                     PDKAPQECTYAQLEVVPAAEVARIFGGG"
     gene            complement(2729..>3021)
                     /locus_tag="VFQ35_05305"
     CDS             complement(2729..>3021)
                     /locus_tag="VFQ35_05305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9930076.1"
                     /translation="VFGPEALIARLGGDEFAVFVTSVDTATMHSATTRLEETVAVINA
                     SSTTRYRLSISMGVATNPPHKDIAALMQAADAAMYEAKRLRHQRRSLRVLTA"
BASE COUNT          542 a         1002 c          991 g          486 t
ORIGIN      
        1 atgcatcgcg cgatcgagca gaaaaagaag acttacacgc tgccatggca gatggatctg
       61 ctacggcacg tgatggtgaa cgcgccggag tggctactga ggcgcctcgc gccgccgcct
      121 cgtcgcgaag gcgcggcgca atgaagtgac gccccgcacg cggcgcgcga cgtgtgtcgc
      181 gatgaggctc caagcgcaag catgcctcac gcccatatct cggcaggcga ccgtttcagg
      241 tgtacgctgg catcgtgagc cgggaagaag cgccccacgt ttttgagctc gacggacacg
      301 aagtcgcagt cacccaccca agcaagccgt atttttcgcg gagcgcgcag gtcacgaagc
      361 tcgagctcgt gcagtactac ctttcggtgg ccgaaggcgc attgggcggc attcgcaatc
      421 gacccatcgt gctcaagcgc ttcgtgaacg gcgccgaacg tgaggccttt tatcagaagc
      481 gcgctccgga aaagcggccg agcttcattc ggactacgac gctctcgttc cccagtggcc
      541 gaaccgcgga ggaggtcgtc gtggacgacc gcgccggtct cgcctgggtg atcaatctcg
      601 gttgcatcga gctgcatccc cacgcggtgc gcgcctccga tttggagcac cccgacgagc
      661 ttcgcgtcga cctggatccg ggccccgggg tcgcttgggg agacgtacgg cgcgtcgcgc
      721 tcgaggtcaa cgcgctgctg agcgagctcg ggctcaaggg ttttccgaag acgagcgggt
      781 cgcgcggcat gcacgtgaac gtacgtatcg agccacgctg gtcattcggc gaggttcgac
      841 gcgcggcgct cgccctctcg cgggaagtgg agcgacggct accgcagctt gctacctcca
      901 agtggtggaa ggaagagcgc catggggtgt tcctcgacta caaccagaac gcgaaggatc
      961 gaacgacctg cagcgcgtac tcggtgcggc cgctccccga cgcgcgcgtc tcgacgccgc
     1021 tcgaatggag cgaggtgccc gactgcgaac ccgcggattt cacgctgttc acggttccaa
     1081 ggcgcttcgc tgagcgcggg gacccgcacg ccgggatcga cgccgaggcg ggttcgctcg
     1141 aacccttgtt ggagctcgcg cgtcgcgacg aagcagcagg gctgcccgat gcgccttggc
     1201 cgccgcactt tcgcaagatg gaaggtgagg cccctcgcgt cgctccctca cgagcccgcg
     1261 ccaaagcgag ctcggcgtcg gaaggcgaag caaagaagac ccgcacgcgc tctccacgca
     1321 gcaagatgcc gttgctcacg gtcgccaact cacccgacaa agccgccgcg atcgccgggc
     1381 tcgaacgctg gaaggccaag taccccgaag caagcactca cctcgtcgcc gaggacgtgc
     1441 tcgtcgacgc aatgcgaggc cggttctcga cgtggacgag agtccgcgtg aaccttcggc
     1501 acgtgccgga agcgctacgt cctcctcaag agacgcccga ccccgacgac gatcccacgc
     1561 gcgagtggcg cgagcgccgc gctcgctgat gatgctcagc ctccgccgaa gatacgagcc
     1621 acttcggccg cgggcaccac ctcgagctga gcgtaggtgc attcctgggg cgccttgtct
     1681 ggacgaaaac gccggaactg cgccgtgtgt cggaagcgcg agccttccat gtgatcgtag
     1741 ccaacctcga cgacgagctc gggccggagc ggctcccagg acaaatcctt gccctgactc
     1801 cagcggctct gaccgccggg catgcgctgt tcgccgccaa cgccttcggc ggaaccctgc
     1861 tctgcccagc gccgccacgg atgcttggcg agcgcgttct cgcgcagcgg cgcgagctcg
     1921 gagaccaact cccgacgtcg cacctgcgtg aaactcgcgc acacgccgac gtggtgcaga
     1981 tcaccgtcgt cgtcgtacaa cccgagcaag agcgacccaa ccagcgtctg gttgccttgc
     2041 ttgtgccagc gaaatccggc caccacgcag tcgcactcgc gttcgtgctt caccttgaac
     2101 atcacgcgct tgtcgggctc gtagcgcccg gtcttgggct tcgccatcac gccgtcgaga
     2161 ccggcgcctt cgaaacgctg gaaccaatcg gaagcgagag cgcgatccgt cgtggcagga
     2221 gtcaggtgca gcggcgggcg ggcgtcgcgc agcacctctt cgagccgctt gcgtcgcgct
     2281 tcgaacggca cgctgcagag atcttccgag ccgaggcaga gcaggtcgaa gaagacgatg
     2341 gatgcggggg tctgctgggc gaggagcttc acgcgggacg ctgcggggtg aattcgctgc
     2401 tgcagcgcgc cgaagtctag tcggtcgccg agcgctacga cgacctcccc gtcgagcacg
     2461 catcgctcgg gcagctgctc cagcaacact ggtaagagct cggggaagta tcggttcagc
     2521 ggcttcttgt cgcggctctg cagggtcacc tcgtccccgt cgcgaaacac gagaacgcga
     2581 aagccgtccc atttgggctc gaaaatgaag gcctcgccct cgggaaggcc actcacgcgc
     2641 ttggcgagca tcggcaagac gggcggattg atcggcagct gcacgcccgc agcctagcgc
     2701 acttttctcg aggttcggag cgcctcgttc aggccgtgag cacccgcaac gagcgacgtt
     2761 ggtgacgcag gcgtttggct tcgtacatcg ctgcgtccgc cgcttgcatc agggccgcga
     2821 tgtccttgtg aggcggattg gtggcgacgc ccatcgagat cgagagacgg taccgggtcg
     2881 tcgaagatgc gttgatgacg gcaacggtct cttcgaggcg cgtggtcgcg gagtgcatcg
     2941 tcgccgtatc caccgaggtc acgaacacgg caaactcgtc gccgcccagc cgcgcaatga
     3001 gcgcttcggg gccgaacacc t
//
LOCUS       DASTKS010000256         5317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_932500, whole genome shotgun sequence.
ACCESSION   DASTKS010000256 DASTKS010000000
VERSION     DASTKS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932500"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1643)
                     /locus_tag="VFQ35_05310"
     CDS             complement(393..1643)
                     /locus_tag="VFQ35_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930077.1"
                     /translation="MQPRTRPGAGGFLIPALPAWSQVNRIYIVGAVLGSAAMRFPFPS
                     LLVSSSVIGCSLALCLACDTNSNSNNNGQGGSPASGGSSSGGSSNAQGGATNSGGTAS
                     SSGGSSTSGGNSANGGSTSGGATSGGAANGGNSNGGSSNGGSSNGGSGMAAGGKTSGG
                     AGGVGGGASGGGSNASGGNGGNGGASSGGCAKGADTIFCEDFETVPTGTVAKTNGYSP
                     VTNNGTLTVDSTHARGMRALHVHTDGNGKAYIQLSPFAPPSNSFFGRMYIWVTAFPSA
                     PDYAHFTLVEAAGTGAGVIRPIGGQYIPGKGQLWGAGSDSGPTGDWTNWKESAPAQAG
                     KWLCMEWEMTAADNNINIWIDSTAKTDLSVSTKMHGGSSVDFVFPTFNNVWFGWWLYQ
                     SGPTPNQFDLWIDDISLGTKRQGC"
     gene            complement(1757..2809)
                     /locus_tag="VFQ35_05315"
     CDS             complement(1757..2809)
                     /locus_tag="VFQ35_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET9930078.1"
                     /translation="MFIPTVAVRLYRPVALALEAEGIDAARVFAEFDMPDPATTGWDV
                     RLPLPQLAGVWGRLLEVTGDPQFALRAAEYVDLTVCDVITFLEGNAKTVRTALEAKFK
                     FLPLITNAVEWTLETSDDEARVTLFERPPRPPLAPVAEYLLGTRNVFFRRFGPKAWGL
                     RDVSFRHAAPPNVALYQQVFGVVPRFDAALDQLVFDRSLLDAPMQNRDDALTELLSRY
                     AEPALAKADQPATLSGRIEALLSSGVDPGVVSIAERLGVAPRTLQRTLSNEGTTYAEI
                     ATRMKRAAAERLLRRRELAIAEVAYALDFSDASAFHHAFVRWTGVTPGEFRARNLGDL
                     FSEPNHGRLGPRSAAE"
     gene            3219..4091
                     /locus_tag="VFQ35_05320"
     CDS             3219..4091
                     /locus_tag="VFQ35_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930079.1"
                     /translation="MLASDYPVRGKVRAEPERAAVPLKWSVFTLIGLLFCMSVVGVIR
                     PQIYARESANWAAQAVGQDWVDLVFAVPSLVAVAVLANRGSRAGRLLLAGGLLYCVYE
                     LAIYAFAVHFNALFLVYVAAFGVSVGSLVVALRDLFEEDVRGWFSARVPTKGPAVFLA
                     LIGAGFALLWLGEIVPALTAGTLPASVAEASVPTNPVHVLDLSLILPAHLVAAVMLFR
                     RRRAGYVAAPVLLAFGVLMALSIGGMLVVMNLRGASQGFGVALAMLGVSAASALFLVR
                     MLRTLNDVRVGEWH"
     gene            complement(4100..5164)
                     /locus_tag="VFQ35_05325"
     CDS             complement(4100..5164)
                     /locus_tag="VFQ35_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930080.1"
                     /translation="MLRTTLLKAFSAALVSVLTVNVAWAETADATIAETLFREGQDLM
                     QRGRVAEACAKFQASQTAQPALGTLLNLAVCHEREGKTASAWTEFSEVQATAARTQDE
                     KRRAYAETHLKQLANKLSKVEIHVVEPTPELRITLDGRVLPSAAWSSLIPVDPGEHTL
                     EASAPGFRTFSEKRQFEAKAGTTAWQVPTLEALPKEPEPQITPPPLSNATPAPAPLES
                     APVSTPGAPSPAPATSDDRATRRALAWMSGGFGVVGLGLGVALELAAQSYAKDRDELC
                     PPDRGCTTVSEYNRAKDAHDDAKRSQVLGFVSGGVGLAALGAAGYLFWSSRSDAQSVS
                     VHVTPVVGPSVNGLFASGKF"
BASE COUNT          812 a         1764 c         1758 g          983 t
ORIGIN      
        1 cgggcacctg gaccgggagg cgctcatcgc aagtcgcagc ccgtagccga tttacgggat
       61 cgctcgatcc gcctccttct cgcgctgcag gccgaggggt cgtttcggcc ccccgcgcgg
      121 ggcgctggcg cgcgtaagcg gagcgagcac gacaggccgt tcagccacgt gcatcgctcg
      181 gggcagctag tgaatcgccc gtggtggctg agtcggttgg cgacccgctc tgcagccgct
      241 agaaccggcg agctacgaat acgagcttgg cgccggaggt cgaggcttcg ccgtaagagc
      301 cacgatggcc agtcacgtga gtctcgccac ccatccacgg cacgagtggc gagcggacta
      361 gttggagcgg cgagtggctc ttgcttttgc gctcagcagc cttgtcgctt cgttccgagc
      421 gagatgtcgt cgatccagag atcgaactga ttcggcgtcg ggccgctctg atagagccac
      481 cagccgaacc aaacgttgtt gaacgtcggg aacacgaagt cgacgctgct gccgccgtgc
      541 atcttggtcg agacgctgag atccgtcttg gcggtgctgt cgatccagat gttgatgttg
      601 ttgtccgccg ccgtcatttc ccattccatg cagagccact taccggcctg cgctggggcg
      661 gattctttcc aattcgtcca gtcgccggtg gggccgctgt cactgccagc gccccacaac
      721 tgccctttgc cggggatgta ctggccgccg atggggcgaa tgactccagc gccggtgccc
      781 gccgcctcga cgagcgtgaa gtgcgcgtag tcgggtgcgc tcgggaaagc cgtaacccaa
      841 atgtacatgc gaccgaagaa gctgttgctc ggcggggcga agggcgagag ctggatgtac
      901 gccttgccgt tgccgtcggt gtgcacgtgc agcgcgcgca tgccgcgggc gtgcgtcgaa
      961 tcgacggtta aggtgccgtt gttcgtgacg gggctgtagc cattggtctt cgctacggtt
     1021 ccggtcggca ccgtctcgaa gtcttcgcag aagatcgtgt cggcgccttt ggcgcagcca
     1081 ccgctggatg caccgccgtt gccgccgttg ccgccgctcg cgttcgagcc gcctccgctt
     1141 gctccgccgc cgacgccgcc agcgccgccg ctcgtctttc cacctgcagc catcccggag
     1201 ccgccattcg agctgccgcc attcgagctg ccgccattcg agttgccgcc gtttgcagcg
     1261 ccgccactgg ttgcgccacc cgaggtcgaa ccgccgttcg cgctgttgcc ccccgaagtg
     1321 ctgctgccgc cgctagacga cgcggttccg ccgctgttcg tcgcgcctcc ctgggcattg
     1381 ctcgaaccgc ccgagctgct cccgccggac gctggactgc caccctgacc attgttgttg
     1441 gaattggaat tcgtgtcgca cgcgagacaa agagcgagcg aacagccaat taccgacgac
     1501 gagacgagca gtgaaggaaa cgggaagcgc atcgccgcgg agcctagcac ggcgccgacg
     1561 atgtaaatgc ggttgacttg gctccaagcg gggagcgcgg gaataagaaa cccccccgcc
     1621 cccggccgag tacggggctg caccagcccc tccctccagg gcgcctcgcg acgcccccct
     1681 cgaatagcgc gagagcgacg ggccggtcat tgtgcgagcg cgccaaatcg ttgcgcactc
     1741 gcgcccatcc gcggagttat tcggcggcgc tgcgcgggcc caagcggccg tgattgggct
     1801 cggagaacag gtctcctagg ttccgcgcgc ggaattcgcc gggggtgact ccagtccagc
     1861 gtacgaacgc atgatgaaag gccgacgcgt cgctgaagtc gagtgcatac gcgacctcgg
     1921 ctatggcgag ctcacgacga cgtagcaaac gctccgccgc tgcgcgcttc atgcgcgtcg
     1981 cgatctccgc gtacgtggtg ccttcgttgc tcagcgtgcg ttgcagcgtg cgcggcgcga
     2041 ccccgaggcg ctcggcgatg ctgaccacac ccgggtcgac gccggagctc aagagcgcct
     2101 cgatgcgtcc gctaagcgtc gccggctgat ctgccttggc gagcgccggc tccgcgtagc
     2161 gactcaagag ctcggtcaac gcgtcgtcgc gattttgcat tggtgcgtcg agcaagcttc
     2221 ggtcgaacac cagttgatcg agcgccgcgt cgaagcgtgg cacgacaccg aagacttgct
     2281 ggtagagcgc gacgttcggc ggcgcggcgt ggcggaagct cacgtcacga agcccccacg
     2341 ctttcggacc gaagcgcctg aagaagacgt tgcgagtccc cagcagatat tcggcaacgg
     2401 gcgccaacgg tggacgcgga ggccgctcga agagggtgac gcgcgcctcg tcgtcgctag
     2461 tctcgagtgt ccactcgacc gcattcgtga tgagcggcaa gaacttgaac ttcgcctcga
     2521 gcgcagtccg tacggtcttc gcgtttcctt cgagaaacgt aatcacgtcg cacacggtga
     2581 gatcgacgta ctccgccgcg cgcaacgcaa actgcgggtc gccggtgact tcgagcagtc
     2641 gcccccaaac cccagcgagc tgcgggagcg gcagtcgtac atcccagcct gtggtcgccg
     2701 gatccggcat gtcgaactcg gcgaagacgc gggcggcgtc gatgccttcc gcttcgagcg
     2761 cgagggcgac gggtcgatag aggcgaacag cgaccgtggg gatgaacacc ggcggagact
     2821 acagtaactc acccgcttgt caccccggat ggtaaaggcg cgcgtcgccc gcccgcactc
     2881 ttcgaccgag cagcgctcat tttttcatct ttgctgcgac gaatggcggg cggaaactgg
     2941 gatgccccca ccgtccagtg ctcgccgctc tgcggctgcg cgcttcctgg ctcccccaaa
     3001 cgtcgtgctc gccgctccgc ggctgcgcgc gccgtggggg agcccaacgc gctcgcccct
     3061 gtcaccgagc tccccgcgca gccaacggaa gaacgacgca ggaagtgcct tgctcggctc
     3121 gcgcgcgcag cgcttgcacc gccgatcacg cggaggaaca cgcttcgcgc ggcttcgtcc
     3181 cgcgaccgcg ttggcacggc gaatgccacc gaccgggcat gctggccagc gactatcccg
     3241 tacgaggcaa agtccgcgct gaacccgagc gcgctgcggt cccgctgaag tggtccgtat
     3301 tcaccctgat agggctgctc ttctgtatgt cagtagtcgg ggtgattcgg ccgcaaatct
     3361 acgcgcgcga gtccgcgaac tgggcagcgc aagcagtcgg acaggactgg gtcgatctcg
     3421 tgttcgcggt cccgtcgctc gtcgcggtcg cggtgctcgc aaatcggggg tcgcgcgccg
     3481 gtcggctgct gctcgcgggc ggcttgttgt attgcgtcta cgagctcgcg atctacgcct
     3541 ttgcggtcca tttcaacgcg ttgttcttag tgtacgtggc agcctttggc gtttcggtcg
     3601 gatcgctcgt cgtcgcgctg cgagatctct tcgaagaaga cgtgcgcggc tggttttcgg
     3661 ctcgagttcc cacgaagggc ccggcggtgt tcctcgcgct catcggtgca ggcttcgctc
     3721 ttctgtggct cggcgagata gttccggctc tcaccgccgg gacactgcct gcctccgtgg
     3781 ccgaggcttc ggtgcccacc aacccggtgc acgtcctcga tctctcgttg atcctcccgg
     3841 cgcacctcgt ggcggcggtg atgctctttc gccggcgcag agcaggctat gtcgcagctc
     3901 cggttttgct cgccttcggc gtcctcatgg cgctctcgat tggaggcatg ctcgtcgtca
     3961 tgaatctgcg cggcgcgtcg caaggcttcg gggtcgccct ggcgatgcta ggcgtgtctg
     4021 ctgcgagcgc gctgtttcta gtgcgcatgc tgcgcacgtt gaacgatgta cgcgtaggtg
     4081 agtggcactg aagccgctct caaaacttgc cgctcgcaaa gagcccgttc accgacggcc
     4141 ccaccactgg cgtcacgtga acgctcacgc tctgggcgtc gctgcgcgaa gaccaaaaca
     4201 ggtatcccgc ggctccgagc gcggcgagcc cgactccgcc gctcacgaat cccaaaactt
     4261 gagagcgttt ggcatcgtcg tgggcgtcct tggcgcggtt gtactcgctc acggtcgtgc
     4321 aaccgcgatc aggcggacag agttcgtcgc ggtctttggc gtacgattgc gcggcgagct
     4381 ccaatgcgac tccgagtccg agcccgacga cgccgaatcc gcccgacatc catgcgaggg
     4441 cgcgccgcgt cgcgcggtca tccgaggtcg ctggggcggg cgagggcgct cccggcgtcg
     4501 acacaggcgc gctctcgaga ggcgccggcg ccggcgtagc gttgctcagc ggcggcggag
     4561 tgatttgcgg ctcgggctcc ttgggtagcg cctcgagcgt gggcacttgc caggcggtcg
     4621 tgccggcttt cgcttcgaac tgccgtttct cgctgaaggt gcggaagccg ggggcgctcg
     4681 cttcgagcgt gtgttctcca gggtcgactg ggatcaggct gctccacgcg gcgctcggca
     4741 gcacacggcc gtcgagcgtg atgcgaagtt ccggtgtcgg ctcgacgacg tggatctcga
     4801 ccttgctaag cttgttcgca agctgcttca gatgggtctc ggcgtatgcg cgccgcttct
     4861 cgtcttgggt gcgcgcggcg gtggcttgca cctcggagaa ctcggtccag gcactcgcgg
     4921 ttttgccctc gcgctcgtgg cacacggcca agttcaagag cgtgccgagc gcgggttgag
     4981 cggtctgact cgcctggaac ttcgcgcaag cttcggccac gcgcccgcgt tgcatcaggt
     5041 cttgcccttc gcgaaacaga gtctccgcga tcgtggcgtc tgcggtctcc gcccacgcga
     5101 cgttcaccgt gagcacgctg acgagggcag ccgagaaagc tttcagcaag gtcgttctga
     5161 gcattacgcg gtccttacag gctcgtatcc gtcgcaacta cgccgctcac gagagcgctg
     5221 ttgaatgtgg caatcgtcga atgcgtccgg aaccaaatga agccggcgcc gcctccgcca
     5281 gctccggcgg cagcgccgtc tccgctcgac gtgccgt
//
LOCUS       DASTKS010000257         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_487744, whole genome shotgun sequence.
ACCESSION   DASTKS010000257 DASTKS010000000
VERSION     DASTKS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487744"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFQ35_05330"
     CDS             complement(<1..223)
                     /locus_tag="VFQ35_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HET9930081.1"
                     /translation="MRIFGRRSVRYIAQMEMAECGAACLAMVLGFHGRHVSLAEAREA
                     CGVSRDGSSAYAIARAAESFGLSVEALSLD"
     gene            complement(225..1496)
                     /locus_tag="VFQ35_05335"
     CDS             complement(225..1496)
                     /locus_tag="VFQ35_05335"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9930082.1"
                     /translation="MTSPIGVTLEKKDAVAPPERAGVLRLFRREALEHYYNAESETEA
                     QVLRVSPPWTWALFLSFAIIIVVAVAISCVAEVDITSRGRGVMRAPGGVRVLQAQVGG
                     VVADVKAKSGQPVEAGQPLLRLESAVLEGALLEADRKLVQMRSNLAAFVARQETLYRQ
                     RTDLLTKRAGILREREESLDSTIERLNKRVQSYDILGQKGIVGSMALDDAAEQLAQAE
                     RLRLATREELSQAKLELAALESGQQSDRFRWEQEVQDAETKRNALAFSLNQTSISAPQ
                     AGYLEAVLVRPGDVIQPGSAVGRLVAGGSPSKIIAFLPERDRAFVTSGAEIRVEVEQL
                     PYAEFGTLRGRVDRVASDLAARYEVTEALGEEAQYPGPSYRVELTLLEDDRAKQLASK
                     LRAGMLVNARFTLRRRRLISLVLDPLRRWLR"
     gene            1732..>2262
                     /locus_tag="VFQ35_05340"
     CDS             1732..>2262
                     /locus_tag="VFQ35_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET9930083.1"
                     /translation="MQVFGRDYSQTKSYSAGTHRARSPEETLRDYERYMPELGITRLA
                     NITGLDCVGIPVYLCVRPNARSLSVSQGKGVDNCAAKASALMESLENWHAERIRLPLR
                     FESYAALKREGAVMDIERLPLRRGGKLALDRPMMWLEGWDILQNRAVWVPHEFVSMNT
                     VRPLQSGMTFYVSTNGL"
BASE COUNT          425 a          767 c          687 g          383 t
ORIGIN      
        1 gatcgagact gagcgcttct acgctgagcc caaagctctc ggcggcgcgg gcgatcgcgt
       61 aagcgctgct cccgtcgcgc gacacgccgc aagcctcgcg cgcctcggcc aaggacacgt
      121 gccgcccgtg aaagccgagc accatcgcga ggcaggcggc tccgcactct gccatctcca
      181 tttgcgcgat gtagcgcaca ctgcgacgtc cgaagatacg catgtcaacg caaccagcgc
      241 cgcagtggat cgagcacgag cgaaatcagc cggcgccgac gcaacgtgaa gcgcgcgttc
      301 accaacatgc cggctctcag cttcgacgcg agctgtttcg ctcgatcgtc ctcgagcaac
      361 gtgagctcca cccggtagct cggccccgga tactgcgctt cttcaccgag cgcctcggtg
      421 acctcgtagc gcgctgctag gtcactcgcg acgcggtcga cgcgccctct cagggtacca
      481 aactccgcgt acggcagctg ctcgacctcg acccgaattt cggcaccgga agtgacgaat
      541 gcgcgatcac gctccggtaa gaacgcgatg attttcgagg gggagccgcc cgcgaccaag
      601 cgaccgaccg cgcttccggg ctggatgacg tctcccgggc gcaccaacac ggcttcgagg
      661 tagcccgctt gcggcgcact gatactggtc tgattcaacg agaacgcgag cgcgttgcgc
      721 ttggtctccg cgtcctgcac ctcttgctcc caacgaaagc gatcgctctg ctgcccggac
      781 tcgagcgcgg ctagctcgag cttggcctga gacagctcct cgcgcgtcgc gagtcgcagg
      841 cgctcggctt gtgcgagttg ctcggcggcg tcgtcgagcg ccatcgagcc gacgatgccc
      901 ttctgcccga ggatgtcgta cgactgaacg cgcttgttca ggcgctcgat ggtcgagtcg
      961 aggctctcct cacgctcgcg caagatgccc gctcgtttcg tgagcaggtc ggtgcgctgc
     1021 cggtacaacg tctcttgccg agcgacgaac gccgccaagt tcgagcgcat ttgaaccaat
     1081 ttgcgatcgg cctcgagcag cgcaccctcc agcaccgcgg attccaggcg aagcagcggc
     1141 tgccccgcct cgaccggttg acccgacttc gccttgacgt ctgcgacgac gccgcccacc
     1201 tgcgcctgca gcacccgcac accacccggg gcgcgcatca cgccgcgccc gcgcgaagtg
     1261 atgtcgacct cagcgacgca cgaaatggcg accgcgacga cgatgatgat cgcgaacgag
     1321 agaaagagcg cccaagtcca aggcggcgat actctcagca cctgcgcttc ggtctcgctc
     1381 tcggcgttgt aatagtgctc gagagcctct cggcggaaca aacgcagcac accggcgcgc
     1441 tccggcggcg cgacggcgtc cttcttctcc aacgtgacac cgatgggaga cgtcaactgc
     1501 gccagcctac cgttcccgct ccatcccgca ataggcattc aattgatgaa gagctagcga
     1561 acgtcgaatg aattcacacg cgcgctcggg acgaggggaa tgattcgcgc aacgaaccga
     1621 agcgaggcgc tcttgcgctg catagcgatt cgtttggaga cacgtattgc ctatatcggg
     1681 cctccgttcg cctgcgttac gttgcaagga gctcacgcaa gagagtgccg tatgcaggta
     1741 ttcggtcgcg actactcaca gacaaagagt tactctgccg ggacccaccg cgcgcggagt
     1801 ccggaagaga cgctgcgcga ctacgagcgc tacatgccag agctcggcat tactcgcctc
     1861 gccaacataa cggggctcga ttgcgtgggg atccctgtct acttgtgtgt acggcccaac
     1921 gcccgttcgc tgtccgtctc gcaaggaaaa ggcgtcgaca actgcgccgc caaagcctcc
     1981 gcgctcatgg agtcactcga gaactggcac gcggaacgta tccgcctgcc gctccggttc
     2041 gaaagctacg ccgcactgaa acgcgagggc gcggtcatgg atatcgagcg cttgccgcta
     2101 cgccggggag gcaagctggc cctcgaccgg cccatgatgt ggctcgaggg ctgggacatc
     2161 cttcaaaatc gcgccgtgtg ggtgcctcac gagttcgtca gcatgaatac ggtgcggccg
     2221 ctgcagagcg gaatgacctt ctacgtgagc acgaacgggc tc
//
LOCUS       DASTKS010000258        10180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1156541, whole genome shotgun sequence.
ACCESSION   DASTKS010000258 DASTKS010000000
VERSION     DASTKS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156541"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFQ35_05345"
     CDS             <1..650
                     /locus_tag="VFQ35_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9930084.1"
                     /translation="GIEHEFLPPLELSVEGFYKDLRNLVVSNPAADQSAAGQTYTNVG
                     TGRSYGAEVLLRYKPTGRFFGWLAYTLSRAERKDGPADDKHLFEYDQTHILTAIASYK
                     LGRGWQIGSRFRYVSGKPYTPNLGGVMDYDAGVYSPIASPNEYSKRLPSFHQLDVRVD
                     KTWKFSHWSLAAYLDVQNVYAHKNIEGRADNYDYSKTTPIYGLPILPVVGLRGEL"
     gene            647..1570
                     /locus_tag="VFQ35_05350"
     CDS             647..1570
                     /locus_tag="VFQ35_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930085.1"
                     /translation="MKNLLLKTLVSSLALGALFGCEEAVDPGSRVTSLRVMAQRMDAP
                     YAAPGETVHVESLALEPAGRPVNWAWALCENPVSTDVEGCIAQIASDGPTQLMTGTGL
                     DTVDVTVPADALSSLPAAARPSATVGVLSVACPGNIEFAPGKTGYPFRCSDAESGREL
                     ALNEFVVGLKRIAVRAMDRNQNPSIEGITFDGADWPEDLVPTVTACKSAGNDFADCDS
                     GFAHKIRVQVTPESFETGRTEFGEEFSEQLVVQYFATEGIFESDARTAESPENKWAAR
                     AQSVGRDVNLWFVARDNRGGVTWASRRVHDE"
     gene            complement(1663..3522)
                     /locus_tag="VFQ35_05355"
     CDS             complement(1663..3522)
                     /locus_tag="VFQ35_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930086.1"
                     /translation="MRSTSPLLLFAFLFASPHVALATTYYVGPAGTASAAGTSRDAPS
                     TPAAIVDKLAPGDVVIFLDGVYPTKETTKITKSGTAQAPITFRADEGAIPVFRGPGET
                     PDQGAIEAGADVGFLVFEGLWFENWGSGAIELDWDFKGCHDITMRYNVADSNGRGGFA
                     PYNSDNALIEYNISSRNGYRSDGSWSSNINVWGTSGKVVVRGNVAFHSVDTSSNNSDG
                     NGFIADLTLANTQTLFENNIAFGNGGACIAITDSGNATLIGNTCYNNNKRAPTNDFNF
                     VNTCRSGSVDGVPVNGKGWTFSGLTMRNNLLAGGVKTISSCGGSSYTQQDNVTNASNV
                     FTDPAAGDFRPKAGSAAVDKVAHGTTFATDIGFDPKCIKVETDSAKKHGYSWWTNAPD
                     FDYMKKIGGIKKCWAPRNRPQGSNQELGAYEVSTSGCTTNAECADTDPCTDDSCSPEG
                     ECKHAPLSSCGGGGGSGGSGGGAGGAAQSGGAQNRGGDSGSPRGGATGQGGGAQGGSD
                     NSRGGTSNGTSSGGSANGGSANGGSAMASGGSNPAQGGASTVGSGGSAQPGLGGSTSG
                     QAGKPGAAAGSSSGGGDSGGCSYSAPSRSSRGAGVVSLLLALFAVRRRRVRRS"
     gene            4067..4657
                     /locus_tag="VFQ35_05360"
     CDS             4067..4657
                     /locus_tag="VFQ35_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930087.1"
                     /translation="MLSSDNSLLVLVALGVVVFVALLSVAWLASTSSRRRHEHLKRRF
                     GPEYERAIVEYGSLERAERELIAREKRVKHLRLHDLSSNERARFSGNWEAVQARFVDD
                     PSGAVQAAHELIERVMVARGYRLEDFDQRIADLSVEHAAVVQHYRAARVLADANREGR
                     ANTEELRQALVHYRVLFADLLAPPSHGRQQLKEARV"
     gene            complement(4697..6694)
                     /locus_tag="VFQ35_05365"
     CDS             complement(4697..6694)
                     /locus_tag="VFQ35_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET9930088.1"
                     /translation="MGSLSSCRKRGEEQRPATKPAPAASAAPSARAVERADTVTHASE
                     PYDAGPPVVATSSVDGASLRARHIERLKSDRSPVTVLRGDGPLSLGQRICEAVVPARP
                     KATPILLKPNICGFDGFKNVEKTGDDGVSGRVTDAEFVRGVVRCLKARGHERITIAEG
                     CGNSHEHWKKAALVSGFEAMAREEGVPLVALDDDGVFDREGDTPGKPLAIRGIERTRV
                     PTLLMPKLLAETLEQGLFISVPKLKAHRFAVVSLGIKGMQGTVMRSEAAPAYNQKWRM
                     HEELKPYLGARRRKEPEDRAAYVHALELFAERMVDVLEISLPDVVLVDGAPAVAGDGF
                     QKVRKLPGYLAIGGTNPVLVDRVGSQYLGLWNNAALGAELGGHRSSPLIEAAARRYGL
                     DLKEPALGGDGVALLATPPPVYFKAIAPFSLEGEKVVRAVPPDAGVADSGPAPSAAAT
                     SPASAADSRPIAHAIHFAAPPRIDAVVEASWSQAPVVLWDTDYAGKPTGIVTQARFAW
                     REGALYALFELAGTELNVDTSFPVAVERPKLYREDCVELFFTPDPSRPKHYYEVELGP
                     FGHFADIEVDRESRREGVDWSSRLEIAASHDAARKTALIEVRFGSEDMTRAVRPGARL
                     PFALYRMEGRSPRQYLAWSPPRTPKPNFHVPEAFGTLVIEP"
     gene            complement(6776..8197)
                     /locus_tag="VFQ35_05370"
     CDS             complement(6776..8197)
                     /locus_tag="VFQ35_05370"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9930089.1"
                     /translation="MSTRSRSLAWLQFLVALLVLVWGAAGRASPQELHQVSGSVFSFS
                     PLPWQSPFRYGPFDKLSPDARFARLMARFRERLQTKHVPGGSVAIVLDGHLAYSAGVG
                     LLRSEGEARVSAHSRFLTASLSKMVLAATIMSLAERDGLVLDQPLKAELPYFARGDGY
                     DPSLVTLRTLLDHTSGVPDTGDPRPLPLRALIEAHATDPLLSPPGRLFNYSNAGYALL
                     AAAVEARTARPFEQVVAERIFGPCSMRDAGYDLGRAPQRVCAGHDASGKVIWSRPSDS
                     SAAHAAGGVIASVVDFAHFAEMLLARGGSVLSTASVDAMMEGHALAQDEPKRTYGYGL
                     FASEHAGLPIIEHAGSAAGFSSLVRLVPSRNFAVVVFDNGPFAPDELADAAMSAFLDV
                     PEVPRARVQTLPESWEEYVGRYGDAVGALGEFEIALRGGHLWFERKAGRPLPSTLRGR
                     FERDASGKIQYFVTRMGVAERRP"
     gene            complement(8316..9053)
                     /locus_tag="VFQ35_05375"
     CDS             complement(8316..9053)
                     /locus_tag="VFQ35_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9930090.1"
                     /translation="MRFIVMHKVDAQMEAGAPPDQSIIGQMGQLVQESIKAGTFKNGA
                     GLHRSASRARLVFRGGKRTVTRGPYAGKNELVNLVLMVQTPSMDSAIDQAGRVAAVLG
                     DVEIEVGPVVEAWDLGMMPKPADLKRERFLLLVKGDAETESGQRDAKRRAALAELEQK
                     LFDEGILLARERLAPTSRASRVAPGQRGRQAWTDGPFAESKELVAGFSILELPTKEDA
                     LAWADRYAAILVRNEVDVIELEDPAAG"
     gene            9416..>10180
                     /locus_tag="VFQ35_05380"
     CDS             9416..>10180
                     /locus_tag="VFQ35_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9930091.1"
                     /translation="MKPPSLSATVALPPSAFRTIVDSGPVAVLVWDRAGRILGTNTET
                     RRLLGYDEGELEGRQVGALVPAEYEATVEEFLAALWTEPKPLPASDAPEIRMVRKDGS
                     DVWVQLGLQRVRTDDGDYAVGAMISVDARRRAELAQRESEQRFATAFRVSPDVMSISD
                     IETGRYIEVNEAHERAFGYTRAESIGKSPLELGIIASNTVPNEMVELLERDGHIRNLR
                     VEGHTRGGETRIMLLSAEVIDIAGRRCVVRSTHDVTD"
BASE COUNT         1795 a         3409 c         3271 g         1705 t
ORIGIN      
        1 tcggcatcga gcacgaattc ctgccacccc tcgagctctc ggtcgaaggg ttttacaaag
       61 acctccgcaa cctggtcgtt tcgaaccccg ccgcggatca gagcgccgcc ggtcagacct
      121 acaccaacgt gggaaccggc aggagctacg gcgcggaggt cttgctgcgc tacaagccca
      181 ccgggcgctt cttcggctgg ctcgcgtaca cgctgtcacg cgccgagcgg aaggacgggc
      241 ccgccgacga caagcacctg ttcgagtacg atcagacgca cattctcacc gcaatcgcga
      301 gctacaagct cgggcggggc tggcaaatcg gaagccgctt tcgctacgtc tcgggcaagc
      361 cgtacacgcc gaacctcggc ggcgtcatgg actacgacgc cggcgtctac tccccaatcg
      421 cttcgccgaa cgagtactcg aagcgcttgc cgtcttttca tcagctcgac gtgcgcgtcg
      481 acaaaacctg gaagtttagc cactggtcac tcgcagcgta cctcgacgtc cagaacgtct
      541 acgctcacaa gaacatcgag gggcgcgccg acaactacga ctactccaaa acaaccccca
      601 tctacggact ccccattctc ccggtcgttg gcttgcgagg cgagctatga aaaatctgct
      661 gctcaaaacc ttggtctcat cgctcgctct cggagcgctg ttcggctgcg aagaagcggt
      721 cgaccccggt tcgcgcgtga cgtcgctgcg ggtcatggcc cagcgcatgg acgcgccgta
      781 cgccgcgccg ggagaaaccg tgcacgtcga gtcgctcgcc ctcgagcccg ccggacgccc
      841 cgtcaactgg gcttgggcgc tgtgcgagaa ccccgtatcc accgacgtcg agggctgcat
      901 cgcgcaaatc gcgagcgacg gccctactca gctcatgacc ggcaccgggc tcgacacagt
      961 ggacgtgacg gttcctgccg acgcgctgag ctcgttgccg gccgcggcac ggccgagcgc
     1021 gacggtcggg gtcctcagcg tcgcctgccc tggcaacatc gaattcgcgc ctggaaagac
     1081 cggctatccg ttccgatgta gcgacgccga gagcggtcgt gagctcgcgt tgaacgagtt
     1141 cgtggtcggc ttgaagcgca tcgccgtgcg agcgatggat cgtaaccaaa acccgagcat
     1201 cgaagggatc accttcgacg gagccgactg gccagaagat ctggtgccga ccgtgaccgc
     1261 atgcaaatca gccggcaacg acttcgcgga ctgcgactcc gggttcgcac acaagatccg
     1321 cgtacaggtc actcccgaaa gtttcgaaac gggtcgcacc gagttcggcg aggagttcag
     1381 cgagcagctc gtggtgcagt acttcgcgac cgaaggcatt ttcgagagtg atgcgcgcac
     1441 ggcagaaagc ccggagaaca agtgggcagc gcgcgcacag tcggtaggtc gagacgtgaa
     1501 tctctggttc gtcgcgcggg acaaccgggg cggagtcacc tgggcaagcc gtcgcgttca
     1561 cgacgagtga agcaacgcaa ccgcaccgag cccctcatct tcacgcgcgc cagtggcgtg
     1621 acggtgaggg gcagtcgggc acgcgaagag ccgcagatct ggctagctac gccggacgcg
     1681 tcgccggcgc accgcgaaga gggcgagcag gagtgacacc acccccgcgc cgcgagagct
     1741 ccgcgacggc gccgagtagg agcacccgcc cgaatcgcca ccgccgctcg agctcccagc
     1801 cgccgcaccg ggctttccgg cttggccgga ggtgctgccg ccgagcccgg gttgcgcgct
     1861 tccgccgctt cccactgtcg aagcgccacc ttgagccgga ttggaaccgc cactggccat
     1921 cgcgctgccg ccgttcgcgc tgccaccgtt cgcgctgcct ccactcgagg tgccgttcga
     1981 tgtgccgccc cgcgagttgt cgctgccgcc ctgagcgccg ccgccctggc cagttgcgcc
     2041 tccgcgcggc gagcccgagt caccgccgcg gttctgcgcg ccaccgcttt gcgccgcacc
     2101 gccagcgccg ccgcccgaac caccgctacc accgccgcca ccgcagctgc tgagcggcgc
     2161 gtgtttgcat tcaccttcgg ggctacacga atcgtcggtg cacgggtccg tatcggcgca
     2221 ctcggcgttc gtcgtgcagc ccgaggtcga gacttcgtac gcgccgagct cctggttgct
     2281 gccttggggt cggttgcgcg gcgcccaaca cttcttgatg ccgccgatct tcttcatgta
     2341 gtcgaagtcg ggcgcgttgg tccaccacga gtagccatgc ttctttgcag agtcggtctc
     2401 gaccttgatg cacttcgggt cgaaaccgat gtcggtcgcg aaggtcgtcc cgtgcgcaac
     2461 cttgtcgacg gcggccgagc ccgccttggg ccgaaaatct cccgccgcgg gatccgtgaa
     2521 cacgttcgac gcgttggtca cgttgtcctg ctgcgtgtag ctcgagccgc cgcagctcga
     2581 gatcgtcttc acgccacctg cgagcaggtt gtttcgcatc gtgaggccgg aaaaggtcca
     2641 gcctttgccg ttcaccggga cgccgtcgac gctgcccgag cggcacgtat tgacgaagtt
     2701 gaagtcgttg gtcggcgccc tcttgttgtt gttgtaacaa gtgtttccga tgagcgtcgc
     2761 gttgcccgag tcggtgatcg cgatgcacgc gccgccattt ccgaaggcga tgttgttctc
     2821 gaacagagtt tgagtgttcg cgagcgtcaa atcggcgatg aagccgttgc cgtcactgtt
     2881 gttcgaggac gtgtcgacgg agtggaatgc gacgttcccg cgaacgacga ccttgccaga
     2941 cgtgccccaa acgttgatgt tgctcgacca ggagccgtcg gagcgataac cgttgcggct
     3001 gctgatgttg tactcgatga gcgcgttgtc ggagttgtac ggcgcgaacc cgccgcgccc
     3061 gttactgtcc gcgacgttgt accgcatcgt gatgtcgtgg cagcccttga agtcccaatc
     3121 gagctcgatg gctccgctgc cccagttctc gaaccagagc ccctcgaaca ccagaaagcc
     3181 gacgtccgcg cccgcttcga tcgcgccctg gtccggcgtt tcgcccgggc cgcggaagac
     3241 cgggatcgcg ccttcgtcgg cgcggaacgt gatcggcgcc tgagcggtac ccgatttggt
     3301 gatcttcgtc gtttccttgg tcgggtaaac gccgtcgagg aagatcacga cgtcgcccgg
     3361 cgcgagcttg tcgacgatgg cggctggggt cgacggcgcg tcgcgcgacg tgccggccgc
     3421 gctagcggtg ccagctggcc ccacgtagta cgtggtcgca agcgcgacgt gaggagaagc
     3481 aaagagaaac gcgaacaaga gcagcggcga agtcgagcgc atgattggac ccatccctcg
     3541 tcgaacgaag agggcgcgag tcgcaacccg ctcctccgcg cgaggtggag aaaggatata
     3601 cccgatgacc ccgatcgtga tcacgtttct ccggaatcga agcgtcgatt gctcagcgga
     3661 actcgtcgct tggctaacga attcgtaatg tgttgtgttc gagtggtaaa agggcgaccg
     3721 cgacggactg actcccagcg cgcgtcgacg cgtatcgact cgatggaact caatgtcccg
     3781 tgcgctgagc ccgcatcaat ccttcgatgc gggaatgcgc gctccgagca ttcacctcgt
     3841 tcgtcctgtc gggcatgcgc agaagcgctg gcgagaatcc gggaatttca tcggtgcgcc
     3901 accggcagcg cgaggcgagc ttgcggagcg cggcagtcct gctgacggac aaatccggcg
     3961 cgagcacaaa acgcgcgggg acgagccgaa tctccggaaa tagccgctcg atgcccgttc
     4021 tgtactactg gcacgacggt tgcccctccc tcgacgaggt caggaaatgc tctcgtcgga
     4081 taactcgctt ctggttttgg tcgcgctcgg ggtcgtcgtg ttcgtcgcgc tgctttcggt
     4141 cgcgtggctc gcgtcgacgt cgtcgcggcg ccgccacgaa catctcaagc ggcgcttcgg
     4201 gcccgagtac gagcgggcca tcgtcgagta cggcagcctg gagcgcgccg aacgcgagct
     4261 catcgcccga gaaaaacggg tgaagcacct ccgcctgcac gacctgtcga gcaacgagag
     4321 agcacgcttt tcagggaact gggaggcggt gcaggctcgc ttcgtcgatg acccgtccgg
     4381 tgcggtgcag gccgcgcacg agctcatcga gcgcgtgatg gtcgcgaggg gctaccgact
     4441 cgaggatttc gaccaacgca tcgccgattt gtcggtggag catgcagccg tggtgcagca
     4501 ttaccgcgcc gcgcgtgtgc tcgccgacgc caaccgcgaa gggcgcgcga atacggagga
     4561 gctccgtcag gctctggtcc actaccgggt gctcttcgcc gacttgctcg cgccgccttc
     4621 ccatgggcgc cagcaactca aggaagcgcg cgtctgaagc cgagggggcg tccgggtaaa
     4681 agttcggcgt ctctgctcac ggctcgatga cgagcgtacc gaacgcctcc ggaacgtgga
     4741 agttcggctt gggggtgcgc ggagggctcc aagccaggta ctggcgcggc gagcgcccct
     4801 ccatacggta aagggcaaac ggtaagcgcg ctcccgggcg aacagcacga gtcatgtcct
     4861 ccgaaccgaa gcgcacctcg atgagcgcag tcttccgagc ggcgtcgtgc gaagccgcga
     4921 tttcgaggcg actcgaccaa tcgaccccct cgcgtcggct ttcgcggtct acctcgatat
     4981 cggcgaagtg cccgaaagga ccgagttcga cctcgtagta gtgcttgggt cgcgagggat
     5041 ccggagtgaa aaagagctcg acacagtcct cgcgatagag ttttgggcgc tccacggcga
     5101 cgggaaagga cgtgtccacg ttgagctcgg tgccggcgag ctcgaacaag gcgtagaggg
     5161 cgccctcacg ccaggcaaag cgtgcctgcg tgacgatgcc ggtcggtttt ccggcgtagt
     5221 cagtgtccca aagaaccacc ggagcctggc tccaagacgc ttctacgacc gcgtcgatgc
     5281 gtggaggtgc ggcgaagtgg atcgcgtgtg cgatcgggcg agagtccgcg gccgaagccg
     5341 ggctcgtcgc cgccgcgctc ggcgcgggcc ccgaatcagc cacgcccgcg tcgggaggca
     5401 cggctcgcac caccttctcg ccttccagcg aaaacggtgc aatcgccttg aagtagaccg
     5461 gtggcggcgt cgccaagagc gcaacgccat ctccgccgag cgccggctcc ttcaagtcga
     5521 ggccgtaacg gcgcgcagct gcttcgatga gcggtgagct tcgatgtccg ccgagctcgg
     5581 caccaagggc ggcgttgttc cacaacccga ggtattgcga gccgactcgg tcgacgagca
     5641 ccgggttcgt cccaccgatc gccaagtagc cggggagctt gcgaactttc tggaagccat
     5701 ctccagccac ggcaggcgcg ccgtcgacca gcaccacgtc aggcaacgag atctccagaa
     5761 cgtcgaccat gcgttcggca aacagctcga gcgcgtgtac gtaagcggcc cggtcctccg
     5821 gctccttgcg gcgacgcgcg ccgaggtagg gcttcaactc ctcgtgcatg cgccattttt
     5881 gattgtacgc gggcgccgcc tccgagcgca tcacggtgcc ctgcatcccc ttgattccga
     5941 gcgagaccac cgcgaagcga tgggccttca gcttgggaac gctgatgaat agcccctgct
     6001 cgagcgtctc cgcgagcaac ttgggcatca gcaaagtcgg aacgcgcgtg cgttcgatgc
     6061 cacgaatcgc gagtggcttg ccgggcgtat ctccttcacg gtcgaacaca ccatcatcgt
     6121 ccagggcgac gagcgggaca ccctcctcgc gcgccatcgc ttcgaagcca gagacgagcg
     6181 cagccttttt ccagtgctcg tgcgaattgc cgcagccttc ggcgatggtg atgcgctcgt
     6241 gcccgcgggc cttcaaacag cgcaccaccc cgcgcacgaa ctcggcatcc gtgacgcgcc
     6301 cactcacgcc gtcgtccccg gttttctcca cgttcttgaa gccatcgaag ccgcagatgt
     6361 tcggcttcaa caagatgggc gtcgctttcg gtcgcgccgg cacgaccgcc tcgcaaatgc
     6421 gttgaccgag cgagagcgga ccgtcgccgc gaagcacggt gaccggcgag cgatcgctct
     6481 tcagtcgttc gatgtggcgc gcgcgcagcg aggccccgtc gacgctgctc gtcgcgacga
     6541 cgggcggccc tgcgtcgtac ggctcgctcg cgtgtgtgac ggtgtcggcg cgttcgaccg
     6601 cgcgcgcgct cggcgcggcg ctcgcggccg gagctggttt cgtcgcaggc cgctgctctt
     6661 cgcctcgctt gcggcacgaa gacagcgagc ccacgagccc aatgatccca agcgcacatc
     6721 caagccgttt caacgcggcg agcatagcgc gccggtcgcg cctctcacaa cgcggtcacg
     6781 ggcgtcgctc ggccacgccc atccgcgtga cgaaatactg gattttgccg ctcgcgtcgc
     6841 gctcgaaacg cccgcgcagc gtgctcggca acggccggcc cgctttgcgc tcgaaccaga
     6901 gatggccgcc gcgcagcgcg atctcgaatt caccgagcgc gccgacggcg tcgccgtacc
     6961 ggccgacgta ttcttcccag ctctccggaa gcgtctgaac gcgcgcgcgc gggacctcgg
     7021 gaacatcgag gaaggcgctc atcgcggcgt cggcgagttc gtcgggcgcg aaggggccgt
     7081 tgtcgaagac caccaccgcg aagtttcggc tcggcacgag gcgcacgagg ctcgaaaagc
     7141 ccgcggcgct accggcatgc tcgatgatgg gcagtcccgc gtgttcgctc gcgaagaggc
     7201 cgtagccgta agtcctcttc ggctcgtctt gcgccagcgc atgcccctcc atcatcgcgt
     7261 cgaccgacgc ggtgctcagc acggagccgc cccgcgcgag cagcatctcg gcgaaatgcg
     7321 cgaagtcgac gacgctcgcg atgacaccgc cggccgcgtg ggctgcgctg ctgtcgctgg
     7381 gacgcgacca aatcaccttt ccgctggcgt cgtgccccgc acagactcgt tgcggcgccc
     7441 gaccgaggtc gtagccggcg tcgcgcatgc tgcaggggcc gaagatgcgc tctgccacga
     7501 cctgctcgaa gggccgcgcc gtacgcgctt cgaccgccgc agcaagcagc gcgtaaccag
     7561 cgttcgagta attgaacaag cgcccaggcg gcgacaacag cggatcggtc gcgtgtgcct
     7621 cgatgagcgc gcgcaacggg agcggccgtg ggtctcccgt gtccggaaca cccgaggtgt
     7681 gatcgagcag agtgcgaagg gtgacgagcg acgggtcgta gccgtccccc ctcgcgaaat
     7741 acggcagctc cgctttcagc ggttggtcga gcacgagccc atcgcgctca gccaggctca
     7801 tgatcgtcgc cgcgagcacc atcttgctga gcgaggcggt caaaaagcgc gaatgcgcgc
     7861 tgacgcgtgc ctcgccttcc gaccgcaaca gtccgacccc ggccgagtaa gcgaggtgcc
     7921 catcgagcac gatcgcaacc gagccgccag gcacgtgttt ggtctgcagc cgctcgcgga
     7981 agcgcgccat caagcgggca aagcgggcgt cgggcgaaag cttgtcgaac ggaccgtatc
     8041 gaaacgggct ctgccagggc aaaggcgaaa atgagaaaac ggagcccgat acctgatgga
     8101 gctcctgcgg cgaggctctc cccgctgcac cccaaaccag aaccagcagc gcgacgagga
     8161 actgcaacca cgcgagcgaa cggcttctcg tgctcacgga tgaggagtac cacgttcggc
     8221 gctccgccgc gcgttgcgac aggcgttggg tgcgtcgagc agcgcccgag agcgccgcct
     8281 gtcgccgctc gaccacccca acctcacagc gccggctacc ccgcggcagg atcttccagc
     8341 tcgatcacgt cgacttcgtt tcgaaccaag attgctgcgt accggtccgc ccacgcgagc
     8401 gcgtcctcct tggtcggtag ctcgaggatg gagaagccgg caacgagttc cttcgactct
     8461 gcgaaaggcc cgtccgtcca cgcttgcctg cccctctgcc ccggagccac gcgcgaggcg
     8521 cgcgaagtcg gtgctaggcg ctcccgcgcg agcaggatgc cttcatcgaa cagcttctgc
     8581 tcgagttcgg cgagcgccgc gcggcgtttt gcatcgcgtt ggccgctctc cgtctccgca
     8641 tcgcccttca ccaacaggag gaaccgttcg cgtttcaggt cggcgggctt cggcatcatg
     8701 ccgagatccc acgcttcgac cacgggaccc acttcgattt ctacgtcccc cagcacggca
     8761 gccacgcgcc ccgcctggtc gatagccgag tccatggaag gggtctggac catgagaact
     8821 aggttcacga gctcgttttt gccggcatac ggaccgcgcg tgacggttcg cttcccccca
     8881 cggaacacga gccgcgcgcg agaagcgctc cgatgcaagc ccgccccgtt cttgaaggtg
     8941 cctgccttga tgctctcctg aaccagctgc cccatctgcc cgatgatcga ttggtcgggg
     9001 ggagcaccag cttccatttg ggcatccact ttgtgcatca cgatgaaacg catcgaacga
     9061 ttctcctgat ttcgaggtga gaaatctcaa ttgctgcggc gcggcggact ccccgtccct
     9121 acacggttcc gttggggcag gccagagacg ccaaaccgtg agaacgcccc ttggggaatc
     9181 cgcgtgacga ttcggtttgg ctggtccgcc atcgcaaggt cgacgactgg cggccgtcgg
     9241 gatcgacaac gctcgattac gattttcgaa tgatccgacc ctagccgctc ttggcgcata
     9301 gcggcattcc gggcgaccgc agcgattgcg ggcaaaaatg cctcgccacg tagtccagcg
     9361 ggccggcacc gtgcgatact gcgcgggctg ccgcgtgagg gatcgaaatc aggggatgaa
     9421 accaccgagc ttgtcagcga ccgtcgcgct cccaccgtcg gcattccgta caatcgtgga
     9481 ctcggggccc gtagcggtac tggtttggga ccgggccggg cgcatcttgg gaaccaacac
     9541 cgaaacacga cggctcctcg gctacgacga gggagagctc gaaggcaggc aggtcggagc
     9601 gctcgtgccg gctgagtatg aggcgaccgt cgaggaattt ctcgccgcgc tctggaccga
     9661 gccgaagcca ctccccgcga gcgacgcacc agaaatccgc atggtccgca aggacgggag
     9721 cgacgtctgg gtccagcttg gccttcaacg tgtgcgcacc gacgacggcg actacgcggt
     9781 cggcgcgatg atcagcgtcg acgctcgacg ccgcgcggag ctcgcccaga gagagtccga
     9841 gcagcggttc gcgacggcgt ttcgcgtctc tcccgacgtg atgtccatca gcgacatcga
     9901 gaccggacgc tacatcgaag tgaacgaagc ccacgagcga gcattcggct acacgcgtgc
     9961 ggagtcgatc ggaaaatctc cgctcgagct cgggatcatc gcgtcgaaca ccgttcccaa
    10021 cgagatggtc gagctcctcg agcgggacgg gcacattcgc aaccttcgcg tcgaaggcca
    10081 tactcgcggc ggcgaaacgc gaatcatgct gctcagcgcc gaggtgatcg acatcgcggg
    10141 gcgccgctgc gtcgtgcgca gcactcacga cgtcaccgat
//
LOCUS       DASTKS010000259         5948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1380072, whole genome shotgun sequence.
ACCESSION   DASTKS010000259 DASTKS010000000
VERSION     DASTKS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380072"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFQ35_05385"
     CDS             complement(<1..966)
                     /locus_tag="VFQ35_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9930092.1"
                     /translation="MGGSTSNTPPPAYDAAGVDLITNAAACKASSPDVGRTPLRRISR
                     IEYNNIVRDLLGDQSRPADGFVSEEKVVGFNSNSYTAVNPVIMRQYWDAAEKLATAAA
                     GNYQTLLGCGSNVTDSCVQTFIGKFAKRAFRGVYDDATAKALFSVYSDTKTQFDVQTG
                     VQALVTDVLTSPRFLFVFEFGQGSPSGGQLPLSSWELATRLAFFMWRSVPDDTLAQAA
                     DMNQLSTPEQIEAQATRMLGDQKAKSALDDFVNQWLDIENMDTVTKDTMFSNVWSPQV
                     AKDLHTETLTTYSNLVNSGNSFVDLMTSNQSYINSGLGTFYGASSS"
     gene            complement(1659..3236)
                     /locus_tag="VFQ35_05390"
     CDS             complement(1659..3236)
                     /locus_tag="VFQ35_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9930093.1"
                     /translation="MFKRLLAIVGFGLALAPAAAFAEDAAAPSASAAAPAASAAAPAA
                     PVAEPAAAAPAPAPAPAPVAAPVAAAAPAVAAAPSDVEKRLADLEAYVNNGARVDAAS
                     SKVAGAGPGHNGWMMVCAALVLFMTLPGLALFYGGLVRQKNVLSVMAQCLGLAGMVTI
                     LWWAFGYSIAFSGGSPVLGSMKFAFLEGVDAMPNADYGAWVSQNVFAMYQLMFAIITP
                     GLIVGAIAERMKYSSLMLFLLGWMLIVYFPLAHMVWGVDGMMNGVWNAKAAIKAIDFA
                     GGTVVHMSSGWSAIVLAVILGKRKGFGHDNFAPHSLVLTMVGTGMLWVGWYGFNAGSA
                     VAADGVAANAFMTTTLAAAIAGATWPALEWITRGKPTVLGFCSGIVGGLVVITPAAGF
                     VNATSAVIIGVLAGVIPFFACTKLKQLLGYDDALDTFGVHGVGGTLGAFVTGIFATPT
                     VNANLDTNLKGVVGSTLWLEQLKAMALTIVLSVVATVVLAYAIKAVLGLRPTLEDEET
                     GLDLSDHGEKGYHLGET"
     gene            3536..4498
                     /locus_tag="VFQ35_05395"
     CDS             3536..4498
                     /locus_tag="VFQ35_05395"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9930094.1"
                     /translation="MNDPGNAIDAAARALAAGELVAFPTETVYGLGADAEGPSAVRRI
                     FEVKGRPTSHPLIVHVDSVDWLESLAHDVPSIAFRLAERFWPGPLTLILKRSERVPLA
                     VTGGQATVGIRVPSHPVALALLRRFGRGVAAPSANRFGKLSPTTAAHVREDLGDLVAQ
                     VLDGGPCDVGVDSTILDLSREAPRLLRPGGVPREALEEVLGKPLLHETIGPVRAPGLL
                     PSHYAPRAEVVLCSQSELPQRAEALRARGARLGALAPEGTPIALVDVAVTLPGDAVSF
                     ARRLYASLRELDAARVDVILVVAPQVRGLGLAVLDRLTRAAAPR"
     gene            complement(4473..5381)
                     /locus_tag="VFQ35_05400"
     CDS             complement(4473..5381)
                     /locus_tag="VFQ35_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9930095.1"
                     /translation="MQRVLWLTMALNVVVALGKISYGYYADALSVRADGFHSLTDSAN
                     NVVGLVGIRLASRPADEGHPYGHSKFELMAAGLVGLSLLGMAYDVLRNGIERLLDVGV
                     PPTLDSRVFVVLVLTLAINLWVASYERRMGEQLSSAFLLSDATHTRSDVLVTLGVILT
                     VALVELGYPRLDAAAAIGISGFIGWAGLDVLRKNLKHLADARALDPEIIRAVATGIGG
                     VASAHKIRTRGAPGAIYVDLHVQIAPHLDVVMAHRVTHWVIDAIKRDVPGIADVLVHT
                     EPAAEGVPYNPLPDANVDANAAQRRA"
     gene            complement(5454..>5948)
                     /locus_tag="VFQ35_05405"
     CDS             complement(5454..>5948)
                     /locus_tag="VFQ35_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930096.1"
                     /translation="LAGARDREQRRYDLLGEATRSAVDRLVDLGGYRYLGLDPFSLEP
                     LFAESGGQRRSFDVLPTRARHLAAFAALAVRALWAAHPEQDPRDAEGVVAIDDVELYQ
                     DSQALARIGAALREALPRVQWILTTASPVLAASVEASEVIALRRLSESDAVAVFAGDE
                     ALIH"
BASE COUNT         1065 a         1952 c         1981 g          950 t
ORIGIN      
        1 cgatgaggaa gcgccgtaga acgtcccgag gcccgagttg atgtacgact ggttcgacgt
       61 catcaagtcg acgaaactgt taccggagtt gaccaggttc gaatacgtcg tgagcgtctc
      121 ggtgtgtagg tccttcgcga cctgcgggga ccacacgttc gagaacatgg tgtccttcgt
      181 gaccgtgtcc atgttctcga tgtcgagcca ctgattgacg aagtcgtcga gcgcggactt
      241 cgccttctga tcgccgagca tgcgcgtcgc ctgcgcctcg atctgctcgg gggtcgagag
      301 ctgattcatg tccgcggctt gggcgagcgt gtcgtccggt accgagcgcc acatgaagaa
      361 ggcgagacgc gtcgcgagct cccaagaaga gagcggcaac tgaccgccgc tcgggctgcc
      421 ttggccgaac tcgaacacga acaagaagcg cggcgaagtg agcacgtcgg tgaccagcgc
      481 ttgcacgccc gtctgcacgt cgaactgcgt cttggtatcc gagtagacgc tgaagagcgc
      541 tttcgccgtg gcgtcgtcgt agacgccgcg gaacgcgcgc ttggcgaact tgccgatgaa
      601 ggtctgcacg cagctgtcgg tcacgttgct gccgcagccg agcaaggttt gatagttgcc
      661 ggcggcggcg gtcgcgagct tctccgcggc gtcccagtat tgacgcatga tcactgggtt
      721 tacggcggtg tagctgttgc tgttgaagcc gacgaccttc tcctcggaga cgaagccgtc
      781 ggcggggcgc gattgatccc ccaacagatc gcgcacgatg ttgttgtact cgatgcgaga
      841 aattcgacga agcggcgtac gtccgacgtc cgggctggaa gccttgcagg cggcggcgtt
      901 ggtgatcaga tcgacgcccg ctgcgtcgta cgcgggcgga ggggtgttcg acgtcgaacc
      961 gcccatcgag gtcgcgccac cggacgagct actcgaagac ccgccctgcg aagtgttgtt
     1021 tcccgaacct gcagcaggac cgccgccgtt gctcggcgaa gtcgaaccgc cggaactcga
     1081 ggaagtggcg ccgcctgaac cctctcggtc tctgacgctg gattgcgccg gcgagcaagc
     1141 gaagatcgac ggggtgagac caagcccgag gacgagcaag cggcgaagta ccatcgcagt
     1201 ttcggtgtag cagcaaatcg tcggtgtgct gcaccggaaa taacgccgaa aattgtctgc
     1261 aaggaaagcg tcaggactgg tcactcattt tacgagggag cagttccaca cctgcaaatt
     1321 cctttgaacg acattgccca gcgagggctg gtcgaacccg cgtattgccc gataaggcga
     1381 cagttcgcag agagcaccgc gcggctcgaa gcgactctgc gacaaccgtc atgttcctgg
     1441 attaagaaat ctaccgcgga cgaaagcgct attaggcgcc aggcaggcga atagccaagg
     1501 accttaatca accgggtgcc gctctgccgc gcctcaccgc gtgctcgtgg gcgggaaagc
     1561 aacgcaccgc tcggcgaaga caaaaggcaa acgccgcagc ccccgattgc tcggagaact
     1621 gcggcgttcg gtgcaccaac ggacggtgcg gccgccgctc aggtctcgcc caagtggtag
     1681 cccttctcgc cgtggtcgct gaggtcgagg cccgtctctt cgtcttcgag cgtcggacgg
     1741 agaccgagca ccgccttgat ggcgtacgca aggacgaccg tagccacgac actgaggacg
     1801 atggtgagag ccattgcctt cagctgctcg agccagagcg tgctgccaac gacgcctttc
     1861 aggttcgtgt ccaggttcgc gttcacggtc ggggtcgcga agatgccggt gacgaaggcg
     1921 cccaaagtgc cgccaacgcc gtgcacgccg aaggtgtcga gcgcgtcgtc gtagccgagc
     1981 aactgcttca gcttggtgca cgcgaagaac ggaatgacac cagccaggac gccgatgatg
     2041 accgcgctcg tggcgttcac gaagcccgcg gcgggcgtga tgacgaccaa accaccgacg
     2101 atgccggagc agaagccgag gacggtgggc ttaccgcgcg tgatccactc gagagccggc
     2161 caggtggcac ccgcgatcgc ggcggcgagc gtcgtggtca tgaaggcgtt ggcggcgaca
     2221 ccgtcggccg cgaccgcgct accagcgttg aagccgtacc agccgaccca caacatgccg
     2281 gttccgacca tggtgaggac gaggctgtgc ggcgcgaagt tgtcgtgacc gaagccctta
     2341 cgctttccga ggatcacggc gagcacgatg gcggaccagc ccgagctcat gtggaccacg
     2401 gtgccacccg cgaagtcgat cgccttgatg gcagccttgg cgttccaaac gccgttcatc
     2461 atgccgtcga cgccccacac catgtgcgcg agggggaagt aaacgatgag catccagccg
     2521 agcaggaaca gcatcagcga gctgtatttc atgcgctccg cgatcgcacc gacgatcagg
     2581 ccgggggtga tgatcgcgaa catgagctgg tacatcgcga agacgttctg agatacccag
     2641 gcgccgtagt cggcgttggg catcgcgtcg accccttcga ggaaggcgaa tttcatgctg
     2701 ccgaggacgg ggctgccacc ggaaaacgcg atgctgtagc cgaaggccca ccagaggatg
     2761 gtgaccatgc cggcaaggcc gaggcactga gccatcacgc tcagcacgtt cttctgccgc
     2821 acgaggccgc cgtagaagag cgcgagcccg ggcagcgtca tgaacaacac gagcgcggcg
     2881 cagaccatca tccagccgtt gtgaccgggg ccggcgccag cgaccttgga ggaagccgcg
     2941 tcgacgcgcg caccgttgtt cacgtaagct tccagatccg cgaggcgctt ttccacgtcg
     3001 ctcggggcgg cggccacggc gggagcagct gcggccaccg gggccgcaac gggagccggt
     3061 gccggcgcgg gagcaggagc ggcagctgcg ggctcggcga caggagcggc tggtgccgca
     3121 gcggaggccg cgggcgcggc ggcgcttgca gagggcgcag ccgcgtcctc cgcgaaggcg
     3181 gccgcagggg ccagcgccaa cccaaaccca acaatcgcta acaaccgttt gaacatcgcg
     3241 gtctccttcg gaattctgct gccaccggat gacggacctg gggcacccgg gtggcggtct
     3301 gcgtcgtgca gcgcgcccct accgttaccg atccacatgt ttccgatttg cttcgcactt
     3361 gtttcgcgtc acagagccga aaactcggct tgattccggg caatttcgag ctcccgcgcg
     3421 ccgcgctgcg gcacacgacg aagctcggcc gaaacacttt tctggacgcc tccggccccg
     3481 tccgacagcc tcgtcggtgg cggactacat gggaggagct agaacccgca acccaatgaa
     3541 cgaccctggt aacgccatcg acgcagcggc gcgcgccctc gctgcgggag agctcgtggc
     3601 gttcccaacc gagaccgttt acgggctcgg cgccgacgcc gagggcccga gcgccgtgcg
     3661 gcgcatcttc gaggtgaagg gacgtcccac gagccacccc ctcatcgtcc atgtggactc
     3721 ggtcgactgg ctcgagtcgc tggcccacga cgtgccgagt atcgccttcc gcctcgccga
     3781 acgtttctgg ccggggccgc tgacgctgat cttgaagcgc agcgagcggg tcccactcgc
     3841 cgtcacgggc gggcaggcaa ccgtcggcat ccgcgtgccg tcacacccgg tagccctcgc
     3901 actgttacgg cggttcggtc gaggagtggc cgctccctcc gcgaatcggt tcggcaagct
     3961 gagcccaacg actgctgctc atgtgcgcga agacctcggc gacctcgttg ctcaggttct
     4021 cgacggtgga ccatgcgacg tgggggtcga ctcgaccatc ctcgatttgt cgcgtgaggc
     4081 cccgcggctg ctccgccccg gcggagttcc gcgcgaggcg ttggaagagg tgctcggcaa
     4141 gccgctcttg cacgaaacca tcgggccggt gcgcgcccct ggcctcctcc cctcgcacta
     4201 tgcgccgcga gcggaggtcg tgctgtgcag tcaatcggag ctcccacagc gggccgaagc
     4261 gctccgtgcg cggggcgcgc gactcggagc gctggcgccc gagggaaccc cgatcgcgct
     4321 cgtcgacgtc gcggtcactc tacccggtga cgcggtgagc tttgcccggc gtctctacgc
     4381 gagcctgcga gagctcgacg cagcccgcgt cgacgtcatc ctggtcgtcg ctcctcaggt
     4441 ccgcggcctc gggctcgccg tgctcgaccg gctcacgcgc gccgctgcgc cgcgttagcg
     4501 tcgacgttcg cgtcgggcag cggattgtac ggaaccccct ccgccgcggg ctcggtgtga
     4561 accagcacgt cggcgatccc gggcacgtcg cgcttgatcg cgtcgatcac ccagtgcgtt
     4621 acccggtgag ccatcacgac gtcgaggtgc ggggcgatct gcacgtgcag atcgacgtaa
     4681 atcgcgccgg gtgctccgcg agttcggatc ttgtgcgcgc tcgccactcc gccgatcccg
     4741 gtcgctacgg cgcgaatgat ctcggggtcg agggcgcgcg cgtcggcgag gtgcttgaga
     4801 tttttgcgca gcacgtcgag cccggcccag ccgatgaacc cggagatccc gatcgccgcc
     4861 gcagcgtcga gccgcgggta accgagctcc acgagggcca ccgtcaaaat caccccgagc
     4921 gttaccagca cgtcggagcg cgtgtgcgtc gcgtcgctca gcaagaatgc gctcgagagc
     4981 tgctctccca tccgccgctc gtagcttgcc acccagaggt ttatcgcgag ggtgaggacg
     5041 agcacgacga acacgcggct gtcgagcgtg ggcggcaccc caacgtcgag cagccgttcg
     5101 atgccgttgc ggagcacgtc gtaggccatg ccgagcagcg agagcccgac gagcccggcc
     5161 gccatcagct cgaatttcga atggccgtag gggtgccctt cgtccgcggg ccgactggcg
     5221 aggcgaatgc caaccagccc gaccacgttg ttggccgagt cggtgagaga atgaaagccg
     5281 tcggcccgca ccgaaagcgc gtcggcgtag tacccgtagg aaatcttgcc gagcgcgacc
     5341 acgacgttga gcgccatcgt gagccagagc acgcgttgca cggctttatc gcgtggcgag
     5401 ttcggcggag tcgacgaggg caccctggga gcagacactc tctcacgctt cgctcaatga
     5461 atgagcgcct catcgcctgc gaagaccgct accgcatcgc tctcgctcag gcgtcggagc
     5521 gcgatgacct cggacgcttc cacgctcgcc gccagcaccg gtgacgcggt cgtgaggatc
     5581 cactgaactc tcggcaacgc ttcgcgtaaa gcagcgccga tgcgcgcgag ggcctggctg
     5641 tcctggtaga gctcgacgtc gtcgatcgcg accacgccct cggcgtcgcg cggatcttgc
     5701 tccgggtgcg ccgcccagag cgcccgcacc gcaagcgcag cgaacgcggc caagtgccgc
     5761 gcgcgcgtcg gcaacacgtc gaaagaccgg cgctgaccgc ccgattcagc gaagagcggc
     5821 tcgagcgaaa acgggtcgag cccgaggtag cgataacccc cgagatcgac caaacgatcg
     5881 accgcgctgc gcgtcgcctc gccgagtaaa tcgtaacggc gttgctcgcg gtcgcgcgcc
     5941 ccggccaa
//
LOCUS       DASTKS010000260         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1603539, whole genome shotgun sequence.
ACCESSION   DASTKS010000260 DASTKS010000000
VERSION     DASTKS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603539"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFQ35_05410"
     CDS             <1..217
                     /locus_tag="VFQ35_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930097.1"
                     /translation="GSGRVRIRSEAALAIGRYLGGGFKVLAWFGALVPRALRDAVYSA
                     IAARRKSLGGSLSCALPAPSERARFLA"
     gene            258..1496
                     /locus_tag="VFQ35_05415"
     CDS             258..1496
                     /locus_tag="VFQ35_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930098.1"
                     /translation="MRVVRNPRLGIALLAAGLGCGSCGDVVGQTLLARGGGSGMAASS
                     GGDSNSGGALAAGGTSTGGAFGGTSTSGASGGSTGGSIGGSTGGSIGGSSGSNSSSGG
                     TGKGGGGSVSPCPTGLLGFASVVSGATGGVAGRRVRANTAEDLKAYAAADEPLVIEIA
                     ATIALSEELRPKPNKTFLGIGASGALTGAGFYIKNSENIVIQNLTISRLSPGDNDAIG
                     IESSKYVWIDHCDLATSLDDPLGTYDGLVDITHASDFVTVSWSRLHDHYGVSVIGHSD
                     INADEDTGHLRVTMHHNHFQNVVSATPRVRFGKVHLFNNYFESVRENAVLSQMGADVV
                     LERNYFFGAPLPLVTHYTGPEDGNASSLDDVFDQSGAPMITSPSTWSPGAEYSYRDAW
                     DSAGFVPTIVPACAGVGKTK"
     gene            complement(1501..2250)
                     /locus_tag="VFQ35_05420"
     CDS             complement(1501..2250)
                     /locus_tag="VFQ35_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HET9930099.1"
                     /translation="MLKTIAITGLSAMALVVPTAVAFARREPVKPAPIHRKAAEKAAT
                     AAPPVELEAEAPPSPFASLPSVRIASLGTGESLSIRLYDEAGNVDPESAGKLDALLVD
                     ARDPDHVEQTELDRRTLKLLFRAAYHFNAKQVEVVSAYRKPGRRREGLHGQGRAIDFK
                     LPGVKPAALAAYLRTLPRVGVGVYTHPRTQFVHLDSRDRSFHWLDASPPGRHWRERSL
                     PDSKLAARDASYRENMDWPESCAAYRPVATR"
     gene            complement(2266..>3074)
                     /locus_tag="VFQ35_05425"
     CDS             complement(2266..>3074)
                     /locus_tag="VFQ35_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9930100.1"
                     /translation="PPINNPLDAFNRLFSMLGAPQGELDALRQNRRSVLDTVQSQFAT
                     FSPKLSSDDRQKLDAHLTLVRDLERRMVDLANGRSCAAPSTPPALTPENLDDMPRIAD
                     LELDLLAAAFACDLTRVASFQISTSLNHIAYPFLNGNMMEGHGLSHAGPSDTAAHDEL
                     VSRQTWHSGRLAYFLSRLASFQDPDGSSVLDNTLIVWGNEISEGNTHSHDDIPFVLVG
                     GGWAFKTGRYVKCQGVPHNNLLVTILNAMGVAATTFGDPKFCTGPLSGLV"
BASE COUNT          510 a          947 c         1079 g          538 t
ORIGIN      
        1 gggcagcggt cgcgtgcgca tccgttcgga ggccgcgctg gcgatcggtc gctacttggg
       61 tggaggcttc aaggttttgg cgtggttcgg agcgctcgtg ccgcgtgcgc tccgcgacgc
      121 ggtctattcg gcgatcgccg cgcgacgcaa gagtctcggg ggatcgctga gttgtgcgct
      181 gccggctccg tcggaacgag ctcgctttct cgcctgaacg tggatagctc cgcgcggtgc
      241 tgtaagatgg tggggtggtg cgcgtcgtac gtaacccgcg gctcgggatc gcccttctag
      301 cggcggggct cggctgcggg agctgtggcg acgtcgtagg gcaaacgctg ctggcacgcg
      361 gtggaggttc cgggatggct gcctcgtcgg gcggcgactc caactctggc ggggcgctcg
      421 ccgccggcgg cacctcgacc ggcggcgcct tcggaggaac ttcgacgagc ggcgcctccg
      481 ggggctcgac cgggggcagc atcgggggct cgaccggggg cagcatcggg ggctcctccg
      541 gcagcaattc gtcgagcggc gggaccggca agggcggtgg cggctccgtt tcgccttgtc
      601 ccacggggct gctcggtttc gcgagcgtcg tgagcggagc gacgggaggc gtcgcgggca
      661 ggcgcgttcg cgcgaacaca gcggaggact tgaaagcgta cgcagccgcg gacgagccgc
      721 tcgtcatcga gatcgccgcg accatcgcgc tcagcgaaga gctccgcccg aagccgaaca
      781 agaccttcct cggaatcggc gcttcgggtg ctctgaccgg agccggcttc tacatcaaga
      841 attccgagaa catcgtcatt caaaacctta ctatctcgag gctttctccc ggcgacaatg
      901 acgcgatcgg catcgagagc agcaagtacg tgtggatcga ccactgcgat ctcgcgacgt
      961 ctctcgatga cccgctcggc acctacgatg gtctggtcga catcacgcac gcctcggact
     1021 tcgtcaccgt ttcgtggtca cgcttgcacg atcactacgg cgtgagcgtg atcggccaca
     1081 gcgacatcaa cgccgacgaa gataccggcc acctacgcgt gacgatgcac cacaaccact
     1141 tccaaaacgt cgtctcggcc acgccgcgag tgcgcttcgg caaggtgcat cttttcaata
     1201 actacttcga gagcgtgagg gaaaacgccg tgctttccca gatgggcgcc gacgtcgtgc
     1261 tcgaacggaa ctactttttt ggcgctccgc tgccgctcgt aacgcactac acgggccccg
     1321 aggacggcaa cgcgagctcg ctcgatgacg tcttcgacca gagcggcgcg cccatgatca
     1381 cgagcccgag cacctggtct cccggcgctg aatactctta tcgcgacgct tgggactcgg
     1441 cggggttcgt gcccaccatc gtccccgcct gcgccggcgt gggcaaaacc aaatgaagtg
     1501 tcaacgagtg gccacggggc gatacgcggc gcagctctcc ggccagtcca tgttttcgcg
     1561 gtagcttgcg tcacgggccg cgagcttcga atccggtagc gagcgctcgc gccagtgcct
     1621 gccgggcggt gaggcgtcta gccagtggaa gctgcggtcg cgtgagtcga ggtggacgaa
     1681 ctgagtgcgc gggtgcgtgt acacgccgac gccgacgcgc ggcagcgtgc gcaggtatgc
     1741 ggcgagcgcg gccggcttca cgccgggcag cttgaaatcg atggcgcggc cctggccatg
     1801 cagcccttcg cgacgccgcc ccggcttacg ataagcggag acgacctcga cttgcttggc
     1861 gttgaagtgg taagcggccc gaaacaggag cttcaacgtg cgtcggtcga gctcggtttg
     1921 ctcgacgtga tccgggtcgc gggcgtccac gagcaaggcg tccagcttgc cggcgctctc
     1981 tgggtcgacg ttacccgctt cgtcgtagag cctgatgctc aacgattcgc cggttccgag
     2041 gctcgcgatg cgaacgcttg gcaaagacgc gaacggtgac ggcggcgctt ccgcctcgag
     2101 ctcgactggc ggcgctgcgg tagccgcctt ttcggctgct ttgcggtgaa tcggggcggg
     2161 cttgaccggc tcacgccgag cgaacgcgac ggcagtcggc accaccagcg ccatcgcgct
     2221 cagcccggtg atcgcgatgg ttttcagcat cttggcttcc atcgttcaaa cgaggccgga
     2281 gagagggccg gtgcagaact tggggtcgcc gaaggtcgtc gcggcgacgc ccatcgcatt
     2341 caggatcgtg acgagcagat tgttgtgcgg cacgccctga cacttgacgt aacggcccgt
     2401 cttgaaggcc caacctccgc ctacgaggac gaacgggatg tcgtcgtgtg aatgcgtgtt
     2461 gccttccgag atttcgttgc cccaaacgat gagcgtattg tcgagcacgc tcgagccgtc
     2521 tggatcctgg aacgaggcga gccgcgacaa aaagtaggcg aggcgacccg agtgccacgt
     2581 ctggcgtgag acgagctcgt cgtgggcggc ggtatcggag ggcccggcgt gggagagccc
     2641 gtgcccctcc atcatgttgc cgttcaagaa cgggtaggcg atgtggttca gcgacgtcga
     2701 gatctggaac gatgcgacgc gcgtgaggtc gcacgcaaaa gcggccgcga gcaagtcgag
     2761 ctcgagatcc gcaatgcgcg gcatgtcgtc caggttctcg ggggtgaggg cgggtggggt
     2821 gctgggcgcc gcgcacgagc gcccgttcgc gagatcgacc atgcgccgct cgaggtcgcg
     2881 taccagagtc aggtgggcgt cgagcttttg ccggtcgtcg ctcgagagct tcggagaaaa
     2941 cgtggcaaac tgggactgaa cggtgtcgag caccgagcgc cggttctgac gaagcgcatc
     3001 gagctctcct tgcggcgcgc cgagcatcga gaagaggcga ttgaaggcgt cgagcggatt
     3061 gttgatcggc ggca
//
LOCUS       DASTKS010000261         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1826401, whole genome shotgun sequence.
ACCESSION   DASTKS010000261 DASTKS010000000
VERSION     DASTKS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826401"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFQ35_05430"
     CDS             complement(<1..646)
                     /locus_tag="VFQ35_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9930101.1"
                     /translation="MKDYYSEQGLPEAEVPVCPIVRSLAGQTALVTGANSGIGKAIAV
                     ALGHAGANVVVNYVSREEETVRTLDDVRACGGRAISFRADVSKEAEVQAMFAAAKREF
                     GTVDILVNNAGLQRDARFEDLSEADWDLVIDVNLKGQFLCSREAVREFKRRGVRRDVS
                     VSAGKIICISSVHDVIPWAGHVNYAASKGGVAMMMRSIAQEVAPYRIRVNSISPG"
     gene            890..3595
                     /locus_tag="VFQ35_05435"
     CDS             890..3595
                     /locus_tag="VFQ35_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HET9930102.1"
                     /translation="MPLDREELAPPSRAVVDTAEHQRLRADTHRTENWKRWGPYLAER
                     QWGTVREDYSSHGECWDYFSHDQARSRAYRWGEDGLLGICDRQARLCFALALWNERDP
                     ILKERLFGLTGPEGNHGEDVKELYYYLDATPTSSYLKALYKYPQSEFPYGRLVEENRR
                     RGKHEPEFELVDTGAFDEHRYFDVTVEYAKKSPEDILIRISVENPSAVEARVHVLPTL
                     WFRNTWSWGRNGEGYGDRPRLETLGEHAVLASHPMLGEMVLAFEEDTRNAELLFTENE
                     SNSERLFGVPAPTPYTKDAFHRYVVDGERAAVNPERFGTKAAARFVLTVPAGGKCVIR
                     ARLSAGRAVPTFDDFDVTFAERLREADEFYTQTLPRNVREEEAHVSRQAYAGLLWSKQ
                     FYHYVLEHWLDGDPAQPSPPDGRKRGRNWEWKHVYCRDVISMPDKWEYPWFAAWDLAF
                     HMVPFARIDPEFAKEQLLLMLREWYMHPNGQMPAYEFALSDVNPPVHAWAVWRVYKMT
                     GERGRRDRLFLARCFQKLLLNFTWWVNRKDSEGNNLFSGGFLGLDNIGVFDRSKPLPT
                     GGSLEQADGTAWMAFYSATMLSMALELAQDDPSYEDLASKFFEHFVAIVDAMNTLGGT
                     GLWDEADGFYYDRLNLDGHSTPLRTRSMVGALPLIAVEVLEERAIDRLPGFKKRMNWF
                     LENRRDLARHIAYAELSGSSHGHRLLAVPSRERLVRVLGYLLDEAEFLSPFGLRSLSR
                     VHKERPYVFRDRGAEYTVEYVPGDSNTGLFGGNSNWRGPVWFPLNYLLIEALERYHHY
                     YGDSLEVECPKGSGRFLNLKQVADELSSRLSSLFLPREDGRPCHGSDPRYRDDPRFRE
                     LVLFHEYFHGESGHGVGASHQTGWTALVVRCLESVARSRALRGER"
     gene            3595..>4153
                     /locus_tag="VFQ35_05440"
     CDS             3595..>4153
                     /locus_tag="VFQ35_05440"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HET9930103.1"
                     /translation="MPASEDENAGADRELLDIPVDTQTEWLEADGLGGFASGTTSGVR
                     TRRYHALLLSAARPPSDRFVLVNGFVGWIETEDGREDFTPQHYAPGITTKAPARIVEF
                     KAEPWPTWRFCTQSGVEFTQEIIAQRGSPKVLLRFRLLSGAKARLVVRPLLSGRDFHH
                     LQRENSTFDFTPTVRGQSITFSPYSG"
BASE COUNT          710 a         1273 c         1363 g          807 t
ORIGIN      
        1 ccccgggcga gatggagttc acgcgtatgc ggtacggcgc gacttcttgc gcgatgctgc
       61 gcatcatcat cgcgacgccg cctttggagg ccgcgtagtt gacgtgcccg gcccacggaa
      121 tgacgtcgtg cacggagctg atgcagatga tttttccggc ggacacggag acgtcacggc
      181 gaacaccgcg gcgcttgaac tcgcgaactg cttctcgcga gcagaggaac tgccccttga
      241 ggttgacgtc gatgacgaga tcccaatccg cctcgctgag atcttcgaag cgggcatctc
      301 gctggagccc ggcgttgttc acgaggatgt cgacggtgcc gaactcgcgc tttgccgccg
      361 cgaacatggc ctgaacctcg gcctctttgc tgacgtcggc acgaaaagag atggcgcgtc
      421 cgccacaggc tcgcacgtcg tccaaggtac gcaccgtctc ttcttcgcgc gagacgtagt
      481 tgacgacgac gttggcgcca gcgtgaccga gtgcgacggc gatggctttg ccaatgccag
      541 agttggcgcc cgtgacgagc gcagtttgcc cggcgaggct gcgaacgatg gggcagactg
      601 gcacctctgc ttcgggtagg ccttgttcgc tgtaatagtc tttcattgga gctcctttgg
      661 cttctgagtg cgagaggctc ggttttcccg ggtacgtggc tgcgcggcgc cggttacgtc
      721 tggcggcgct gagtgttggc gttgcaagtt tcgggcgtgc ggagggtaac ccgggtgcgg
      781 cttcctcgta ggtccggcgg gctcggccgt gcgccgggct cgagaaccag ggagcgccat
      841 gaatcacttg caccgtatcg gccgtcgcga tttcctagaa gatgccaaaa tgcctctcga
      901 ccgtgaggag ctcgcgccgc cgtcgcgcgc ggtagtcgac accgccgagc atcagcggct
      961 ccgcgcggac actcaccgca ccgagaactg gaagcgctgg ggcccctacc tcgccgagcg
     1021 gcagtggggc acggtgcgcg aggactattc gagtcacggc gagtgctggg attacttttc
     1081 gcacgaccaa gcgcgcagcc gggcgtaccg ctggggcgaa gatgggctgc tcgggatctg
     1141 cgaccgtcag gcgcggctct gcttcgctct cgcgttgtgg aacgaacgcg atccgatctt
     1201 gaaggagcgc ttgttcggcc tgaccggccc ggaggggaac cacggcgagg acgtaaagga
     1261 gctctattac tacctcgacg cgacgccgac ttcttcttac ctgaaggcgc tctacaagta
     1321 tcctcaaagc gagtttccgt acgggcgctt ggtggaagag aaccgtcggc gcggcaaaca
     1381 cgagcccgaa ttcgagctcg tcgacaccgg cgcgttcgac gagcatcgct atttcgatgt
     1441 cacggtcgaa tacgcaaaaa agagccccga agacattctc attcgcatca gcgtcgagaa
     1501 cccgagcgcg gtcgaggcgc gcgtgcacgt gctgccgacg ctctggttca ggaacacttg
     1561 gtcatggggg cgcaacggcg aaggctacgg ggaccggccg cgactcgaaa ctttgggcga
     1621 gcacgccgtg cttgcgtcgc accccatgct gggcgagatg gtcctcgcgt tcgaggagga
     1681 cacccgaaac gccgagctgc tcttcacgga gaacgagtcc aatagtgagc ggctgttcgg
     1741 cgttccggct ccgacgccct acacgaagga cgcctttcac cgctatgtcg tcgacggtga
     1801 acgggccgca gtgaaccccg agcgtttcgg caccaaggcc gccgcgcgct tcgttcttac
     1861 cgttccagcg ggcgggaagt gcgtgattcg cgctcgcctg tcggcgggcc gcgccgttcc
     1921 gacattcgac gacttcgacg tgaccttcgc cgagcgcctg cgcgaagcgg acgagtttta
     1981 cacccagacg ttaccgcgca acgttcggga agaggaggct cacgtttcgc ggcaagcgta
     2041 cgcgggcttg ctttggtcga agcagttcta ccactacgtg ctcgagcact ggctggacgg
     2101 tgatcccgcg cagccttcac cgccggacgg gcgcaagcgt ggtcgcaact gggagtggaa
     2161 gcacgtttac tgtcgcgacg tcatctcgat gccggacaag tgggagtacc cgtggtttgc
     2221 ggcgtgggat ttggcgttcc acatggtgcc gttcgctcgc atcgaccccg agttcgccaa
     2281 agagcagctg ctcttgatgt tgcgcgaatg gtacatgcac ccgaacgggc agatgcccgc
     2341 gtacgaattt gccttgtccg acgtgaatcc ccccgtgcac gcgtgggcgg tttggcgcgt
     2401 ttacaagatg acgggcgaac gcgggcgtcg cgatcgcttg tttttggcgc gctgctttca
     2461 gaagctgctc ttgaacttca cctggtgggt gaaccgcaaa gacagcgagg gcaataacct
     2521 gttctccggc ggtttcctcg ggctcgacaa catcggagta ttcgatcgca gcaagccgct
     2581 tccgaccggc ggcagcctgg agcaggcaga cggcaccgct tggatggcct tctacagcgc
     2641 gaccatgctc tcgatggcgc tcgagctcgc gcaagatgac ccttcttacg aagacttggc
     2701 gtcgaagttc ttcgagcatt tcgtggcgat cgtggacgcc atgaacacgc tcggtgggac
     2761 tggcctctgg gacgaagcgg acggcttcta ttacgatcga ctgaacctcg acgggcattc
     2821 gacgccgctc cgcacccgct ccatggtcgg cgctttgccg ctgatcgcgg tcgaagtgct
     2881 cgaagagcgc gccatcgatc gcctgcccgg cttcaagaag cgaatgaact ggttcctgga
     2941 gaaccggcgt gacctggcgc gtcacatcgc ctacgccgag ctttcgggga gctcgcatgg
     3001 gcaccgtcta ctggcggttc catcgcgcga acggctggtg cgagtgctcg gctacttgct
     3061 cgacgaggcg gagtttctgt cgccgtttgg gctccgctcg ctcagccgtg tgcacaagga
     3121 gcgcccgtac gtatttcgcg atcgcggcgc cgagtacacg gtggagtacg tgcccgggga
     3181 ctccaacacc ggtcttttcg gcggtaactc gaactggcgc ggccccgttt ggtttccgct
     3241 gaactacctc ttgattgagg cgctggagcg ctaccaccac tactacggag attcgctcga
     3301 ggtggagtgc cccaagggct ctgggcgctt cctcaatttg aagcaagtcg cggacgagct
     3361 ctcgtcgagg ctctcttcgc tgtttctgcc gcgggaagac ggccggccct gccacgggag
     3421 tgacccacgt taccgtgatg atccgcgttt tcgcgagctc gtgctgttcc acgaatattt
     3481 ccatggcgaa agtgggcatg gcgttggggc tagccaccaa actggttgga cggcgctcgt
     3541 cgtgcgttgt ctcgagtcgg ttgcgcggtc gcgtgccctt cgtggagagc gctgatgccg
     3601 gcgtctgaag acgagaatgc aggcgctgac cgcgagctcc tcgacatccc ggtcgatacg
     3661 caaaccgagt ggctcgaggc ggacggtctc gggggattcg cgtccggcac gacgagcggc
     3721 gttcgcacgc ggcgttatca cgccctgctg ctcagcgcgg cgcgaccgcc gagcgaccgc
     3781 ttcgtgctgg tgaacggttt cgtcgggtgg atcgaaacgg aggacgggcg cgaggacttc
     3841 accccccagc actacgcgcc gggtatcact accaaagccc cggctcgcat cgtcgagttc
     3901 aaggcggagc cttggcccac gtggcgcttt tgcacgcaga gcggagtcga gttcacgcaa
     3961 gagatcatcg cgcagcgagg aagcccgaag gtcttgctgc gcttccgcct actcagcggg
     4021 gcaaaggcga ggctcgtcgt ccgtccgctg ctttcggggc gtgattttca ccatctgcag
     4081 cgtgagaact cgaccttcga tttcacgccc acggtcagag gtcagagcat cacgttcagt
     4141 ccgtactctg gcg
//
LOCUS       DASTKS010000262         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_263338, whole genome shotgun sequence.
ACCESSION   DASTKS010000262 DASTKS010000000
VERSION     DASTKS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263338"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1020
                     /locus_tag="VFQ35_05445"
     CDS             247..1020
                     /locus_tag="VFQ35_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930104.1"
                     /translation="MSSAALTATAPHSWLERCGLAAGKVLAPITFATSRVRNARMFHP
                     DGVMYQARVQTCGESPDLYALGMRLAGDAIVRLSSAWWRGKEWPDVLGLAVRFQSPGS
                     QPLTPGPRDQDLLLATVRFPWTTPLAPLATRFGSFLWNHYHAVSPFEAPEIGRVKLRL
                     RSPRIENTGPLSRAEHLERAVVEGRAEWVLEARRLNRMALVRHWEPVAMLTLTRAIDV
                     DQTALRFSPFRTGRDLLPVGFVHYLRVAAYAASQRGRPA"
     gene            1059..2096
                     /locus_tag="VFQ35_05450"
     CDS             1059..2096
                     /locus_tag="VFQ35_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930105.1"
                     /translation="MATSWKKPLLLFSALAIVGAGIAWQRWPLEREPPPPPKAEPAPA
                     PTTIDDPLRPDRAAELSSRAPIGENWVKSAPQKEFQYSKKSGERGVEPCAAPSPEAAK
                     RTLPLSRGYLFLGNDDPASAGDQFDLVFHLNGEGPVRRELVESKQPFVLYTLTLPPTE
                     SYAPLFAGSRLLAHLIDEISAQVSRSRQKPARLRHLALSAWSAGFEGVRSILYQPEAD
                     RVDAVLLIDGLHGPRGGKGLGTQLASFVDFAKRAERGERWFSVTHSSIPTSEFTSTTE
                     SAHFLENELGGKPTRVARDDGFGLELVEMFDKGNLHVRGYAGNDKADHCAQLLLMREL
                     FVALHRYFERR"
     gene            complement(2099..2884)
                     /locus_tag="VFQ35_05455"
     CDS             complement(2099..2884)
                     /locus_tag="VFQ35_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9930106.1"
                     /translation="MTSSEARYPSLARRLYSVWYRHMRVYARDLVSNGLPPFLEPIIF
                     LLGIGFGLGKYVTGMQGLPYVVFLATAMPMTSAMFTASYECTFGTFVRLEFSKTYEGM
                     LAAPLTVTDLFAGELLWAGSKGFFFAACVIAAMTAFGVAPMPGSLFAPFVGALTALAF
                     GALSLLVTSFVKNINHFNFYFTGLLSPMFFFCGAVFPLAELPPALRYASELLPLTHSV
                     RLARALAIRDFAGASVWDAAFLIVFSVAVGALAIARLKRRIIV"
     gene            complement(2871..3809)
                     /locus_tag="VFQ35_05460"
     CDS             complement(2871..3809)
                     /locus_tag="VFQ35_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9930107.1"
                     /translation="MSDAVVIFENVHKAYKGKKAVDGVSFSIERGACFGLLGPNGAGK
                     STLMRLVYGKGRRAPDPPSRVSVLGFDPEKDELSVKCRCGVVPQEDNLDEELNVEQNL
                     FVFARLYGIAHEVARRRVPELLDFMELKEKSRSAVRELSGGMKRRLVIARALLNDPKL
                     LILDEPTTGLDPQVRHVIWDKLRELKRQGLTILLSTHYMEEAFELCEHIVILDEGRVI
                     LAGTPHQLLAEQIERYVLEVVRPEAVERVRARVQALGARVRVDASHETMRFYSDDQQA
                     LLAATEGLAVVDFRLRNCNLEDVFLRATGRGLNDIQ"
     gene            3865..>4159
                     /locus_tag="VFQ35_05465"
     CDS             3865..>4159
                     /locus_tag="VFQ35_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930108.1"
                     /translation="MPARTFGMVFHHYSPRGELHSQRIGEGEVALATSALSFGHQFFD
                     FGVIRTAHGSLGEVEPEHIAELAHDSRPFGDRLGAIDDFPDGGKRSRNVQVGVE"
BASE COUNT          777 a         1368 c         1304 g          710 t
ORIGIN      
        1 atcgcaacga gcgcgcggcc cgcttcggaa agaagcccaa gggcgacccg cgcccgcgtt
       61 gaccatcgcg cgcgagcgaa gccgcacgct gctgaaaacc cgcgggccct tacgaacgtt
      121 ccggaactgg gccggcgctc gttttcgcgc atggcttcgc aagcgttacc ccgagttcat
      181 cgagcgggcg cgagtattgc actctgagct cgcgaggtaa cattgtcgaa cgccatacgc
      241 caatcgttga gctctgctgc cctgaccgcg actgcaccgc atagctggct cgagcgctgt
      301 gggctcgccg ccgggaaggt gctcgcgccc atcaccttcg cgacgagccg cgtgcgcaac
      361 gcgcgcatgt tccatcccga tggggtgatg tatcaggctc gagtccaaac ctgcggggag
      421 agcccagatc tctacgcgct cggcatgcgc ttggcgggcg atgccatcgt gcggctctcg
      481 agcgcgtggt ggcgtggcaa agagtggccg gacgtcttgg gtttggcggt gcgctttcaa
      541 tcacctggct ctcagccgct cactccggga ccacgcgatc aggacctcct gctcgcaacg
      601 gtgcgctttc cctggaccac tccgctcgcg ccgctcgcaa cacgttttgg tagcttcctc
      661 tggaatcact accacgcggt gtctccgttc gaagcgccag agattggcag ggtcaagctc
      721 cgactgcgct cgccgcgcat cgaaaacacg gggccgctct cgcgagcaga gcacctcgag
      781 cgcgctgtag tcgaaggaag ggcggagtgg gtactcgaag cgcgccggct caatcgcatg
      841 gccctcgtgc gtcactggga gcccgtggcg atgctgaccc tgacgcgcgc catcgacgtc
      901 gatcagacgg cgctccgatt ttcgccgttt cgaaccggtc gcgatctttt gccggtgggc
      961 ttcgtacact atctgcgagt cgcggcttat gctgcgagcc agcgcggtcg accggcttga
     1021 tttcgacgcc ggcaaggcgt ttgcttctac ttacacgcgt ggcgacctcc tggaagaagc
     1081 ctctgttgtt gttttcggcg ctcgcaatcg tcggagctgg gatcgcgtgg caacgctggc
     1141 cgctcgagcg cgagccgccc ccaccgccca aagccgagcc cgcgcccgcg cccacgacaa
     1201 tcgacgaccc gctccgcccc gatagagcag ccgagctgtc ttcgcgcgct cccatcggtg
     1261 aaaactgggt gaagtcggct ccacaaaagg agtttcagta ctcgaagaag tcgggcgagc
     1321 gaggcgtcga gccgtgtgct gcaccctccc ccgaagccgc gaagcgtacg ctgcccttga
     1381 gccgcggcta tctctttctc ggcaacgacg atcccgcgag cgccggcgac cagttcgacc
     1441 tcgtctttca tctcaacggc gaagggccgg tgcgccgcga gctcgtcgag agcaagcagc
     1501 cgttcgtgtt gtacacgctc accttgcccc cgacagagag ctacgcgccg ctcttcgccg
     1561 gttctcggtt gctcgcgcac ctgatcgacg agatcagcgc gcaggtgagc cgcagccggc
     1621 aaaagcccgc tcgattacgc cacttggcgc tgagcgcgtg gagcgccggc ttcgagggcg
     1681 tacgctccat cctctatcaa cccgaagccg accgagtcga cgcggtgctg ctcatcgacg
     1741 ggctccacgg gccgcggggc ggcaaaggcc tcggaacgca gctcgcctct ttcgtcgatt
     1801 tcgccaagcg cgccgagcgg ggcgagcgat ggttcagcgt gacccactcg tccattccga
     1861 cgagcgagtt cacgtcgacg acggaatcgg cgcatttcct ggaaaacgag ctcggtggta
     1921 agccgactcg cgtcgcgcga gacgacggct tcgggctcga gctcgtggag atgttcgaca
     1981 agggcaacct gcacgttcgg ggatacgccg gcaacgacaa ggccgaccat tgcgcgcaat
     2041 tgctcctcat gcgtgagctc ttcgtcgcgc tgcatcggta tttcgagcgc cgctgacgct
     2101 acacgatgat gcgccgcttc aagcgggcga tggcgagcgc gccaacagcc acgctgaaca
     2161 cgatgaggaa ggcggcatcc cacacggagg cgccggcaaa atcacggatc gcgagagcgc
     2221 gggcgagccg caccgagtgg gtcagcggca agagctcgct cgcatagcgc agcgcgggcg
     2281 gcaactctgc gagcgggaac accgcaccgc agaagaagaa catcggcgag agcagccccg
     2341 tgaagtagaa attgaagtgg ttgatgttct tgacgaacga cgtcacgagg agagagagcg
     2401 ccccgaacgc gagcgcggtc aacgctccga cgaacggagc gaacaagctc cccggcatgg
     2461 gcgccacccc gaacgccgtc atcgcggcga tcacacacgc cgcaaagaag aaccctttac
     2521 tccccgccca caagagctcg cccgcgaata gatcggtaac cgtcagcggc gcggcgagca
     2581 ttccctcgta cgttttgctg aactcgagac ggacgaaggt accgaaggtg cactcgtacg
     2641 aggcggtgaa catggccgag gtcatcggca tcgcggtcgc gagaaatacg acgtagggca
     2701 agccctgcat gccggtcacg tacttgccga gcccgaagcc aatccccaat aagaagatga
     2761 tcggctcgag gaatggcggc agaccgttgc tcacgagatc gcgcgcgtag acgcgcatgt
     2821 ggcggtacca gacgctgtag agccgacggg cgaggctcgg gtaccgagct tcactggatg
     2881 tcattgagac ctcgtccagt cgccctcagg aacacgtcct ccaagttgca gttcctcagc
     2941 cgaaaatcga cgaccgccag tccttcggtc gcggcgagca aagcctgctg gtcatcggag
     3001 taaaaccgca tcgtttcgtg ggatgcgtcg acgcgcaccc gcgcgcccag cgcttggacg
     3061 cgcgcccgaa cccgctcgac cgcctcgggc cgaacgactt cgagcacgta gcgctcgatc
     3121 tgctccgcga ggagctgatg cggagtgccg gccaagatca cgcggccctc gtccaaaatg
     3181 acgatgtgct cacagagctc gaacgcctct tccatgtagt gagtggagag caagatcgtg
     3241 agcccttggc gcttgagctc tcgcagcttg tcccaaatca cgtgacggac ctggggatcg
     3301 aggccggtcg ttggctcgtc caatatcaag agcttcgggt cgttcaacaa cgcccgcgcg
     3361 atcacgagcc ggcgcttcat gccacccgag agttcgcgca ccgcggaccg cgacttttcc
     3421 ttcagctcca tgaaatcgag aagctcgggt acgcggcgcc gtgcaacctc gtgagcaatc
     3481 ccgtaaagac gagcgaagac gaacagattt tgctcgacgt tcagctcctc gtcgaggttg
     3541 tcttcctggg ggacgactcc gcaacgacac ttcaccgaaa gctcgtcctt ttcggggtcg
     3601 aatccgagca cactgacgcg gctcggcggg tcgggagctc gcctgccctt gccgtagacg
     3661 aggcgcatga gggtgctctt cccggcgccg ttcggcccga gcaagccgaa gcaggcgccc
     3721 cgctcgatgc tgaagctcac gccgtctact gctttctttc ccttgtaagc cttgtggacg
     3781 ttctcgaaga ttacgactgc atcgctcatc ggatcacgtg cagcaaagca catcacggga
     3841 cggccgtagg aactatcgag cgcggtgcca gcgcgcacct tcgggatggt cttccaccac
     3901 tattccccgc gcggtgagct ccactcgcag cgcatcggag agggcgaagt tgcgctcgcg
     3961 acgagcgcgc tgtcgttcgg ccatcagttc ttcgatttcg gcgtcatccg aacggctcac
     4021 ggtagcctcg gagaggtcga gcccgagcac atcgcagagc tcgcgcacga ctctcgcccg
     4081 ttcggcgacc ggctcggggc aatcgacgat ttcccagatg gcggcaagcg ctcgcggaac
     4141 gttcaggtcg gcgtagagc
//
LOCUS       DASTKS010000263         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1156930, whole genome shotgun sequence.
ACCESSION   DASTKS010000263 DASTKS010000000
VERSION     DASTKS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156930"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1056)
                     /locus_tag="VFQ35_05470"
     CDS             complement(49..1056)
                     /locus_tag="VFQ35_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9930109.1"
                     /translation="MTTKQTMHLGKGGPLVFPMALGCMGMGRGSWYGVSDEPESIATI
                     HEAIERGVNVLDTGDFYGMGKNEMLVGRAIQDRRDKVLLSVKYGALRGPDGAFLGHDC
                     RPAATKNALAHSLSRLGVDHIDIYRPARLDPEVPIEDTIGAIAELVQAGYVRYIGLSE
                     VGAETLRRAARVHPICDLQIEYAIVTRNPEGAIIPALRELGIAMTAYGVLSRGLLTGA
                     KPIGKGDGRAHMPRFAEENAQKNAPLVETLSRLAADKGVTPAQLAIAWVRTKSAVQGV
                     GIVPTMGARTRKQLLEALSGLDVTLTDAEVSALETSVPASEVAGSRYPAAAMATLDSE
                     R"
     gene            complement(1344..1760)
                     /locus_tag="VFQ35_05475"
     CDS             complement(1344..1760)
                     /locus_tag="VFQ35_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9930110.1"
                     /translation="MSTPLTTVRALYSALEAGQHGEALRRLFTDDATTLEHPNALKPR
                     GARYSLEEGLAASAAGAGLLESQQFDVQSAVEFEDTAIVRLTWTGVVARDIGPFHAGQ
                     VLTAHIAQFIRVRDARVASIETFDCYEPFSPTTSAS"
     gene            1811..2368
                     /locus_tag="VFQ35_05480"
     CDS             1811..2368
                     /locus_tag="VFQ35_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9930111.1"
                     /translation="MKRLDPASVDLPTLASLAGGAASEHLLEALRGAGHPDVRASHGY
                     VFQLLIDGPKAIGELSSLLGVTQQAASKVALELESLGYVARQAATNDSRVKRVALTPK
                     GKAVIEEARAARTQLEARVRAKVGARAAADARLVLLTLLELTGQFEAVAARRVKPPPA
                     DRDSLGSPGASAARQRRRPRAERRR"
BASE COUNT          452 a          861 c          865 g          448 t
ORIGIN      
        1 gtcacggttt cgcggtgcga gccggcgcag tcgctcacga ggcaaagctc atctttcgct
       61 gtcgagcgtc gccatcgcgg cggcgggata gcggctgccg gccacctcgc tcgcgggcac
      121 cgacgtttcg agcgccgaca cctccgcgtc cgtgagagtg acgtccaggc ccgaaagcgc
      181 ctcgagcagc tgcttgcgtg tgcgcgcgcc catcgtgggg acgatgccga caccctggac
      241 ggcgcttttc gttcgcaccc aggcgattgc gagctgtgcc ggcgtgacgc ccttgtcagc
      301 cgcgaggcgc gacagcgttt ccacgagcgg cgcgttcttc tgcgcgttct cttcggcgaa
      361 tcgaggcatg tgcgcgcgtc cgtcgccctt gccaatcggc ttcgcccccg tgagcagccc
      421 gcgcgagagg acgccgtacg ccgtcatcgc gatgccgagc tcacgaagcg ccggaatgat
      481 ggcgccctcg ggattgcgcg tcacgatggc gtactcgatt tgcagatcac aaatcgggtg
      541 cactcgcgcg gcgcgacgaa gcgtttccgc gccgacctcc gacaaaccga tgtagcggac
      601 gtacccggct tgcaccagct ccgcgatggc gccgatcgtg tcctcgatgg gaacctcggg
      661 atcgaggcgc gctggtcggt agatgtcgat gtgatcgacg ccgagccgcg agaggctatg
      721 cgcgagcgca ttcttggtcg cggcgggccg acagtcatga ccgaggaacg cgccatccgg
      781 gccgcgcagc gccccgtact tcacgctgag aagcaccttg tcgcgccggt cctgaatcgc
      841 cctacccacc agcatttcat ttttgcccat tccgtagaaa tcgccggtgt cgagcacgtt
      901 cacgccacgc tcgatcgcct cgtggatcgt agcgatgctc tcgggctcgt cgctcacgcc
      961 gtaccaactt cctctgccca ttcccatgca accgagggcc atggggaaaa cgaggggccc
     1021 gcccttgccg agatgcatcg tctgcttggt cgtcatggtt tttcagagtc ctcgatgcag
     1081 gttccgcgcg aaatccgtaa cgtgggagtc tgcgcgaact gatggcggca cgaagccgcg
     1141 gtacctcacg aagtcgagca cgcagcccgc cgaacatcca gcccctcgag ctcagaccgc
     1201 taccaaggga gcacgtcacg tggcgggagt cgcagcgaat tgcgaaaacg ccacattctg
     1261 gaggctgcgc cctcatgcga gccgcgcccg gtagacgtcg agcgtggctc atggctcgct
     1321 ggctgcgctc gcccgagggg tcgctagctt gcgctcgttg tggggctgaa cggctcgtag
     1381 cagtcgaacg tctcgatgga agcaacgcgc gcatcgcgga ctcggatgaa ttgcgcgatg
     1441 tgggccgtca gcacctggcc cgcgtgaaat ggcccaatgt cacgcgcgac gacccccgtc
     1501 cacgtcaggc gaacgatcgc cgtgtcctcg aactcgacgg ctgactggac gtcgaactgc
     1561 tgagactcga gtagcccagc gccggccgcc gacgccgcaa gtccctcttc gagcgagtaa
     1621 cgcgcgccgc gtggcttcag cgcgttcgga tgttcgaggg tggtggcgtc gtccgtgaag
     1681 aggcggcgga gcgcttcgcc gtgctggccg gcctccaacg ccgagtacag cgcgcgcacg
     1741 gtggtgaggg gggtagacat atgtacaacc tgacttgtac aaccatggtt gtcaatgtca
     1801 tcgtgtgcgg atgaaacgcc tcgacccagc gtcggtggat ttgcccacgc ttgcctcgct
     1861 cgcaggtggc gcggctagtg aacacctcct cgaagcgcta cggggagcgg gccaccccga
     1921 cgtgcgggcg tcgcacggtt acgtatttca gctgctcatc gacggcccga aggcgatagg
     1981 ggaactgtcg agcttgctcg gggtcacgca gcaagccgcc tccaaggttg ctctcgaact
     2041 cgagtcgctc ggttacgtgg cgaggcaggc ggccacgaac gacagccgcg tgaagcgagt
     2101 agcgctcacc cccaagggca aagccgtcat cgaagaggct cgtgcggctc gcacccagct
     2161 cgaggcacgg gtcagagcga aggtgggcgc gcgcgctgca gcggatgcac gcctcgtcct
     2221 gctgaccttg ctagaactca ccggacaatt cgaagccgtt gccgcgcgtc gggtgaaacc
     2281 ccctcccgcc gaccgcgatt ctctcggcag tccaggcgct tccgccgcga ggcagcggcg
     2341 ccgtccacgc gcagaacggc gccgttgagc attttgcccc gaccgtgctc tagagaaacg
     2401 gctaggagtt cgccggaggt agcggaaaac ggctcggcac ggagtgaccg cgtgggggga
     2461 gtctcttccg aattcatttc ggaagaaggg ggcggcgcgt gccgccccca caaaccaagg
     2521 ctagcgaagc tgctcgatga accccaggcg gtcgacgatt tgaaagtggc cgctgacgcg
     2581 cccgtccgcg aactcgtaga cggtctgacc gtggaaagag agcttt
//
LOCUS       DASTKS010000264         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_488652, whole genome shotgun sequence.
ACCESSION   DASTKS010000264 DASTKS010000000
VERSION     DASTKS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488652"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFQ35_05485"
     CDS             <1..1139
                     /locus_tag="VFQ35_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930112.1"
                     /translation="PKGSAPVAVASLFALPIVGFRRRLFLRGAFLLVLALGILIPGTY
                     DCPLCNRLRYLWPFFPAWLVGAAALTGLVTEEWSRKRSEIAAFGLLALGTLAGALLGY
                     VPMAIDDLAASAAAISKQQVSLGHWAKENLPKGARIGLNDTGAITYFSDRQTFDVVGL
                     TTAGEARYWAAGPGSRFEHYERLGRRKLPTHFIVYPEWFAIDDLFGNELTERYVPGAT
                     ILGGQRMVAYEADYSLLGSAEAPALGDDDAFAILDRLDVADLESEASHRYQLLGSTQR
                     QNYVARSESRLDGARSERRRDDFELTIAPQGALVLRLAADMPTSLTVHVGSRSFEVTL
                     PSAAWHEALLELPSDIPAGRRAISVRSGAGTFSSLHYFSLTRRQ"
     gene            1197..2144
                     /locus_tag="VFQ35_05490"
     CDS             1197..2144
                     /locus_tag="VFQ35_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9930113.1"
                     /translation="MRKRPLGRTSLEVSELSLGTWGLSGDGYGPVPGGDQDGVIDRAL
                     ALGITLFETADCYGEGRMETRLGRRVPPEVEGAVVVTKIGTRRDTPVPRKDFSVPYLK
                     DAVARSQERLRRNVLDCVLLHNPSSQAFDSGELGAFMASLVQEGKVKCWGASVGNTQA
                     GRSAINYGAQVLEIAFNIFHTAEHKELEQQIRSFEIGILARSVLAHGLLCGHWPPTKE
                     FSRADHRSERWSVDELRRRITQLNAVRTLLSEDLPSLRGIALRFALSSDLVSSAVLGP
                     RNWLQLDQLVREAGKEPPYLDPIKLVHLRDRLVTVGVQL"
     gene            2141..2968
                     /locus_tag="VFQ35_05495"
     CDS             2141..2968
                     /locus_tag="VFQ35_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9930114.1"
                     /translation="MKEEEVATLVGIAAEAAELVFRIYQTPFEVDYKGPRDPVTLADR
                     EANALICERLQTAFPGVPVVAEESTPDTFAGYQSSERIFFVDPVDGTREFVAKNGEFV
                     VMIGVVEGERATASVIHAPATGTAWVGLEGHGAFRLSPGEGRTKIAVSECNELAQVRV
                     VASRSHRTPTFERALNELGVGTVLQQGSAGLKGARVAEASADAYVAPFYAGQRWDICA
                     CDALVHAAGGRVTDAYGAPIDYRAPTLAQETGVVVSNGLVHEALLERLALFRARIGR"
     gene            2978..>3197
                     /locus_tag="VFQ35_05500"
     CDS             2978..>3197
                     /locus_tag="VFQ35_05500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9930115.1"
                     /translation="MQSHADVVVIGAGIMGLSLAYNLAGTKPRRSITVVEASYLCSGA
                     SGRNGGGVRAQWANELSVRLMQESLKEFR"
BASE COUNT          523 a         1073 c         1056 g          545 t
ORIGIN      
        1 tgccgaaggg cagcgcgccg gtcgcagtcg cgtcgttgtt cgccctgcca atcgtgggct
       61 ttcggaggcg cctgttcctg cgcggcgcgt tcttgctggt gctcgcgctc ggcattttga
      121 tcccggggac gtacgactgc ccgctctgca accggttgag atatctgtgg ccgtttttcc
      181 cggcttggct cgtgggagcg gcggcgctca cggggctcgt taccgaagaa tggtcgcgca
      241 agcgctccga gatcgctgcg ttcggcttgc tcgcgctggg aacgttggcg ggcgcgctgc
      301 tcggctacgt gccgatggct atcgacgacc tcgccgcaag cgcggcggca atctcgaagc
      361 aacaggtctc gctcggacat tgggcgaaag agaacctgcc gaagggcgcg cgcatcggtc
      421 tcaacgacac cggcgcgatc acctatttct cggatcgcca aaccttcgat gtcgtcggcc
      481 tgaccaccgc cggcgaagcg cgctactggg cagcggggcc aggctcgcgt ttcgagcact
      541 acgagcgact cgggcgacgc aagcttccga cgcacttcat cgtctacccg gagtggttcg
      601 cgatcgacga cctcttcggc aacgagctca ccgaacgcta cgtccccggc gcgacgatcc
      661 tcggcgggca acgcatggtc gcttacgagg ccgactacag cttgctcggc tccgcagagg
      721 cgccagcgct cggtgacgac gacgctttcg cgattttgga tcgcctcgac gtcgcggatc
      781 tcgagagcga agcgagccac cgctaccagc tgctcgggag cacccaacgc caaaactacg
      841 tggcgcgctc ggagagccgc ctcgacggcg cgcgttcgga gcgacgacgc gacgacttcg
      901 agctcaccat cgccccgcaa ggagcgctcg tactgcgctt ggctgccgat atgccaacgt
      961 cactgacggt tcacgtcgga tcgcgctcct tcgaagtcac gctgccgtcc gcagcctggc
     1021 acgaggcctt gctcgagcta ccgagcgaca ttccggcagg ccgccgagcc atctcggttc
     1081 gcagtggcgc gggaacgttc agcagcctgc actatttctc gctgacgcga cggcagtgac
     1141 cccgcaatct gccccgctct ggcccggcgg gcggagccgc tgctataccc tccttgatgc
     1201 gtaagcggcc gctcggcagg acatctctcg aggtgagtga actctcgctc ggtacgtggg
     1261 gcctgtccgg agacgggtac ggcccggtgc cgggcggcga ccaagacggc gtcatcgatc
     1321 gcgcgctcgc gctcggcatc acgctgttcg agaccgccga ttgctacggc gagggccgca
     1381 tggagactcg gctcgggcgc agagtcccgc ccgaagtcga aggcgccgtc gtcgtcacca
     1441 agatcgggac gcggcgagac acgccggtgc cgcgcaaaga cttttcggtc ccgtacttga
     1501 aagacgccgt cgcgcgctcg caagaacggt tgcggcgtaa cgtgctcgac tgcgtgctct
     1561 tgcacaaccc ctcctcgcaa gcattcgaca gcggcgagct cggcgcgttc atggcgagcc
     1621 tcgttcaaga gggcaaggtg aagtgctggg gcgcgagcgt cggcaacacc caggctggcc
     1681 gctcggcgat caactatggc gctcaggtcc tcgagatcgc cttcaatatt ttccacaccg
     1741 ccgagcacaa ggagctggag caacagatcc gctcgttcga gattggcatc ctcgcgcgct
     1801 cggtgctcgc gcatggcctg ctgtgcggcc actggcctcc cacgaaagag ttctcgcgcg
     1861 ccgaccaccg gagcgagcgc tggagcgtcg acgagctgcg ccgccgcatc acccagctga
     1921 acgcggtgcg cacgctgctg tcggaagatc ttccgtcgct gcgcggcatc gcgctcaggt
     1981 tcgcgctttc gagcgatctc gtgagctcgg ccgtgctcgg cccgcgcaac tggctacaac
     2041 tcgaccaact ggtgcgcgaa gcgggcaagg agcctccgta cctcgaccct atcaagctcg
     2101 ttcacctgcg ggatcgcctc gtcacggttg gagttcaact gtgaaagagg aagaggtagc
     2161 caccttagtc ggcatcgcgg cggaagcagc cgagctcgtt ttccgcattt atcagactcc
     2221 gttcgaggtc gactacaagg gcccgcgcga tccggtcacg ctcgcggatc gcgaagcgaa
     2281 cgcgctcatt tgcgaacggc tgcagacggc gttccccggc gtgccggtcg tcgccgaaga
     2341 gagcaccccc gacacgttcg ccggttatca aagctccgag cgcatcttct tcgtcgatcc
     2401 ggtggacggc acgcgcgagt tcgtcgccaa gaacggcgag ttcgtcgtca tgatcggcgt
     2461 cgtcgaaggc gagcgcgcga cggcttcggt gatccacgct cccgccaccg gtacggcgtg
     2521 ggtcgggctc gaagggcacg gcgcgtttcg gttgagccct ggcgaagggc gcacaaagat
     2581 cgcggtgtcg gagtgcaacg agctcgcgca ggtgcgcgtc gttgcgtcac gttcgcaccg
     2641 gacaccgacg ttcgagcgcg cgctgaacga gctcggcgtc ggcaccgtgc ttcagcaggg
     2701 cagcgctggg ctgaagggag cgcgtgtcgc cgaagcctct gccgatgcgt acgtcgcgcc
     2761 cttttacgcc gggcaacgct gggacatctg cgcctgcgac gcgctcgtcc acgccgcggg
     2821 gggccgcgtc accgacgcat acggagcgcc aatcgactac cgcgcaccga cgctcgctca
     2881 agaaactggc gtcgtcgtct cgaacggcct cgtgcacgaa gccttgctcg agcgcctggc
     2941 gctcttccgc gcgcgcatcg gccgctgagg aacgtcgatg caaagccacg ccgacgtcgt
     3001 cgtcatcggg gctggcatca tggggctctc gctcgcctac aatctggcgg gcacgaagcc
     3061 gcgccgctcg atcaccgtcg tcgaggcctc ttatctgtgc agcggcgcga gcggtcgcaa
     3121 cggcggaggg gtgcgagcgc aatgggcgaa cgagctctcg gtgcgcctga tgcaagagag
     3181 cctgaaagag ttccgcg
//
LOCUS       DASTKS010000265         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_42781,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000265 DASTKS010000000
VERSION     DASTKS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42781"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VFQ35_05505"
     CDS             complement(<1..1510)
                     /locus_tag="VFQ35_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930116.1"
                     /translation="MGFLRGRVQGVQSLVVGATALALVALSACGARTGLDPGKVEPVP
                     HDDAGPMCVGSEVQSVRVGSEQQMDLLFVIDNSASMADKQALLSDAVPGMVRRLANPF
                     CVSLTDPKIEKQVASANEPCPAGFRREFEPLTDLHIGVITSALGSAGGGDGDACATGP
                     GHQLEDDQGHLVGTLDRALVVPTYQSAGFLAWDPLGQALPPGEAEMSRLEQNFRDLVM
                     LSGDTGCGFEQPLESWYRFLVDPNPPQTTLVNTSGMAEPVGRDDLLLAQRARFLRPDS
                     LVAVVMLSDENDCSMLGSGQGWLIGKFSFSGQPFRFPRATAACDRDPNDPCCRACINS
                     ESNPPSGCARLKDDPSCAQGEYLEAEDDRFDLRCFDQKRRFGVDLLYPTARYAVGLRN
                     RSLCPSSIYRDADCECTAVKERARRLGLPAPACTSKEVGASVPNPLFTNLTSTPGFER
                     DTSQVFLAGIVGVPWQDLATPETLNDPARLEYLTAGELARRDARLGFTRWELI"
     gene            1653..3188
                     /locus_tag="VFQ35_05510"
     CDS             1653..3188
                     /locus_tag="VFQ35_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9930117.1"
                     /translation="MKPVFSRTQMRQFDQLASEKLRVPSIVLMENAGRGAVDVLLREF
                     GEPSTLRVVVACGAGNNGGDGYVVARRLRLLGAKVEVWSAVAKERLRGDAASNYEAAE
                     RSLGAVKALDDATLADFEKSLVSADVVVDALFGTGLERPIEGTLRAIVECLNAAPAKR
                     FALDLPSGLDADTGEVHGIAVRADATVTFAGMKLGLLTPRGAEYSGRVEVADIGVDAA
                     LLHESGHSARLATDGSASALLKPRAVATHKANSGRVLVLAGSPGKVGAALLVAEGALR
                     GGAGLVTLAGVPALSDALDRRVLEAMTARLDPDHIEASLDALLAATNVVATGPGLGFD
                     AHAKAIVERVVIGHTGVVVVDADAITHFKDSPERLASARGPRILTPHSGELARLLSCS
                     AADVEANRFAALARAVELTRCIVLLKGPHTLVGAPGELPVVGRRGSPALATGGSGDVL
                     GGVIAALACELAPLDAAWTGAYLHALSAEMWSAATQVDRGLVAHEIAAGIPRARSTLQ
                     RTH"
     gene            3522..>4019
                     /locus_tag="VFQ35_05515"
     CDS             3522..>4019
                     /locus_tag="VFQ35_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9930118.1"
                     /translation="MTTPLSNSAECSAPRPAALKPDCTLADRSLAASSHAIRAETASG
                     LRANALGAAELRARSIEIQNGLREHLPALQRHAARRARSREEALDLVQETALRALAFA
                     WSFERGTNLRAWLHQVQDSVFLTQCRKRTRERRAFDAFYLDPCAWPKHGAEPAMQRMT
                     QSGGRA"
BASE COUNT          668 a         1436 c         1262 g          653 t
ORIGIN      
        1 agatcagttc ccagcgcgtg aagccgagcc tcgcgtctcg ccgcgctagc tcgcccgcgg
       61 tgagatactc gagccgcgcg gggtcgttca gcgtctctgg cgtcgccaga tcctgccacg
      121 gaacaccgac gataccggcc aaaaacacct gcgaggtatc gcgctcgaac cccggcgtag
      181 aagtcaggtt cgtgaacagc ggattcggga cgctcgcccc aacctctttg ctcgtacagg
      241 ccggggccgg cagccccagg cgacgcgcac gctccttcac ggccgtgcac tcgcagtcgg
      301 cgtcacggta aatcgagctc gggcacagcg agcgattgcg caagcccacc gcgtagcggg
      361 cggtcggata caaaagatcc acgccgaacc ggcgtttctg atcgaaacag cgaagatcga
      421 agcgatcgtc ctcggcctcc aggtattcgc cttgcgcgca gctcggatcg tccttcaggc
      481 gagcgcagcc cgaaggggga ttggattcgg agttgatgca ggctcggcag cagggatcgt
      541 tcggatcgcg gtcacacgcc gccgtagcac ggggaaagcg aaacggttgg ccactgaagc
      601 tgaacttccc gatgagccag ccctgccccg agcccaacat cgagcaatcg ttttcgtccg
      661 acaacatcac gaccgcgacg agcgagtccg ggcgcaagaa tcgagcgcgt tgggcgagga
      721 gcaagtcgtc gcggcccacc ggctccgcca tcccgctcgt gttcaccaag gtcgtttgcg
      781 gtgggttcgg atcgaccaag aagcgatacc agctctcgag tggttgttcg aagccacacc
      841 cagtgtcgcc gctcagcatc acgaggtccc tgaagttttg ctccaggcgc gacatttccg
      901 cttcgccggg cgggagcgct tgtccgagcg ggtcccaggc gagaaagcca gcgctctgat
      961 aagtcggcac gacgagcgcg cgatccaagg tccccaccaa gtggccctgg tcgtcttcca
     1021 gttggtggcc tgggcccgtc gcgcacgcgt cgccgtcgcc gccgccggcg ctgccgagcg
     1081 cactcgtgat gacgccgatg tgcagatccg tgagcggctc gaactctcgg cggaagccgg
     1141 cgggacacgg ctcattcgcg gaggcgacct gcttttcgat ctttggatcc gtgagcgaga
     1201 cgcaaaacgg gttcgcgagt cggcgcacca tgcccggcac cgcgtcgctc aacagcgcct
     1261 gcttgtctgc catcgacgcc gagttgtcga tgacgaacaa cagatccatc tgttgctcgc
     1321 tgccgacccg gacgctctgc acctccgagc cgacgcacat cggcccggca tcgtcgtggg
     1381 gaacgggctc gaccttgccg ggatccaacc ccgtgcgcgc gccgcacgcc gaaagcgcga
     1441 cgagcgcgag cgccgtggca cccacaacca gagactgaac gccttgcaca cgaccacgca
     1501 gaaaccccat tgcgcctccg gctgggagca accagcgtgc cacgcccgcc gctgctgagc
     1561 cactctgtgc tggtttggcg caacgcggct cgaaagctac agccgaagcg cgccgagccc
     1621 gagctcggtc ggtacctgta tagtctacgc gcatgaagcc cgtcttctcg cgcacccaga
     1681 tgcgacaatt cgatcagctc gcctcggaga aattgcgggt tccgagcatc gtactgatgg
     1741 aaaatgcggg gcgcggcgcg gtcgacgtct tactgcgtga gttcggtgag ccatctaccc
     1801 tgcgcgtcgt cgtggcgtgt ggggccggca acaacggggg tgacggctac gtcgtcgcgc
     1861 gccggctccg gctcctcggt gccaaggtcg aagtgtggag cgccgtcgcg aaggagcgcc
     1921 tgcgcggcga tgcggcgagc aactacgagg cggcggagcg ttcgctcggc gcggtcaagg
     1981 cgctcgacga cgctacgctc gcagacttcg aaaagtcact cgtgtccgct gacgtcgtcg
     2041 tcgatgcgct cttcggcact gggctcgagc gaccgatcga aggaacgctg cgcgccatcg
     2101 tcgagtgttt gaacgctgcg cctgccaaac gcttcgcgct cgacttacct tccgggctcg
     2161 acgccgacac gggcgaagtc catggcatcg cggtgcgcgc cgacgccacc gtgacgttcg
     2221 ccggcatgaa gctcggcttg ctcacaccgc gcggcgccga atactcgggc cgggtcgaag
     2281 tcgcggacat cggcgtcgat gccgcactcc tccacgagtc cgggcacagc gctcgactcg
     2341 caaccgacgg ctcggcgagt gcgctcttga agccgcgcgc ggtcgcgacg cacaaagcaa
     2401 actccgggcg cgttctcgtg ctcgcgggat cgcccggcaa agtcggggcg gcgctgctcg
     2461 ttgccgaagg agctttgcgc ggcggcgctg gtctcgtcac gctcgcgggc gtccccgcgc
     2521 tgagcgacgc gctcgatcgc cgcgtgctcg aagcaatgac ggcgcgcctc gacccagatc
     2581 acatcgaagc gagcctcgat gctctgctcg ccgcgaccaa cgtcgtcgcc acgggtccgg
     2641 ggctcgggtt cgatgcgcac gccaaagcca tcgtcgagcg cgtcgtcatc gggcacaccg
     2701 gggtcgtcgt cgtggacgcc gacgcgatca cccatttcaa agacagccct gagcgactcg
     2761 cgagcgcgcg cggtccgcgc attctcactc cccacagtgg agagctcgcc cgacttctct
     2821 cgtgctccgc tgccgacgtc gaagcgaacc gcttcgcggc gctcgcgcgc gcggtcgaac
     2881 tcactcgctg catcgtactc ttgaaaggtc cgcacacgct cgtcggtgcg ccgggcgaac
     2941 ttccagtcgt cggacgtcgg ggctcacccg cgctcgcgac cggcggctcc ggcgacgtac
     3001 tcggcggcgt catcgcggcg ctcgcgtgcg aactcgcgcc cctcgatgcc gcttggactg
     3061 gcgcttacct ccacgccctc agcgccgaaa tgtggtcggc agccacccaa gtcgaccgag
     3121 gcctcgtcgc gcacgaaatc gccgccggca tcccccgcgc gcgctcgact ctccaacgca
     3181 cgcactgact cgacttctcc aacccgcgca ctgactcgac tctccaaccc gcgcactgac
     3241 tcgactctcc gcgcgcacgt cacgccgcgg aactggttcg agttgaagcc ccgatcgtcg
     3301 atatgcggcc gcccaagtcg gccaccgcct ttggcacgtt gtcattccct ccgtccgccg
     3361 cgcagcgccg gggctgcctt tttgccgact tgtcagctcg tattcggccg ctgacacttt
     3421 gacacgcacc tgcgtccccc tcgccgcatc ccccgaaggc cgaaacgcgc acttttctcg
     3481 cggattcgcg ttggcacacg ccttgcgatg caggactcgc catgacgacc ccgctctcga
     3541 actctgccga atgctccgcg ccgaggcccg ccgcgctcaa gcccgattgc acgctcgccg
     3601 atcgctccct cgcggcaagc tcgcacgcca ttcgtgccga gaccgcgtcg ggacttcgcg
     3661 cgaacgcgct cggcgccgcc gaacttcgcg cccgcagcat cgaaatccaa aatggtttgc
     3721 gagagcacct gcccgcattg cagcgtcacg ccgcgcgccg cgcgcgttct cgcgaagagg
     3781 cgctcgatct cgttcaagag acggctctcc gtgcgctcgc cttcgcgtgg agcttcgagc
     3841 gcggcaccaa cttgcgcgcg tggttgcacc aagtgcaaga cagcgtcttt ctgacccaat
     3901 gtcgaaagcg cacccgcgag cgtcgcgcct tcgacgcctt ctatctcgat ccgtgcgcgt
     3961 ggcccaaaca cggcgcggag ccagcaatgc agcgcatgac ccagtcgggg gggcgcgcg
//
LOCUS       DASTKS010000266         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_710847, whole genome shotgun sequence.
ACCESSION   DASTKS010000266 DASTKS010000000
VERSION     DASTKS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710847"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VFQ35_05520"
     CDS             complement(<1..1545)
                     /locus_tag="VFQ35_05520"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET9930119.1"
                     /translation="MVLRLPRRRFFSLAAVTASSVCAPLGCGSDDASRPDTSAKFFPQ
                     SVASGDPRETSVVLWTRVVDPARPDQDLSVELEVSKDEGFRKVIALNGAAAAELTASA
                     VSDHCLTVRVDSLEAGTTYYYRFRYIGSEGTVTSRTGRTRTAPKADADVQVKFGVVCC
                     QSYAGRYFHVLRQLATYELDFVLHLGDYIYETGRASADGPRAVVFSKPEEALELGGGE
                     LAARSLDNYRDLYRLYRSDPDLQALHERFAMIVIPDDHEFSDDSHGATATYFDERVDE
                     TDVERRMASDQAWFEYMPVDYTDNTASKWQSALEFPDDLRIYRSFAFGKHLELVLTDE
                     RRYRPDHLVPEGASPGVIFALSAALPDVPAEELVPCVNVDIAPFDAYGAELRTHAAEL
                     GLDPSQIAGYVSAPWLNAQLAVVESLLPAIELTEPTLERGYAYHQLLKSAQYGRIGSR
                     YLLAERPFYELARLAVSQRPEAQNILGAEQRAWFLDTMKRSTRTFKVWGSEVAFLSRK
                     LDLTGLT"
     gene            1812..2900
                     /locus_tag="VFQ35_05525"
     CDS             1812..2900
                     /locus_tag="VFQ35_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9930120.1"
                     /translation="MIAIVRLIGVAALALLGVACNKSSSSASGSAESKPSADVAAAAA
                     PAASAAVPTPPPAPSGPALRIAYSDWPGWVAWEIGIQKGWFKEAGVDVDFKWFEYVPS
                     MEAYSAGKVDAVCVTNGDALVTGSNGGKSIGILINDYSDGNDMIVAKPGITSVAQLKG
                     KKIGVEVGFVDHLLLLYALKSANLSEKDVKIVNVPTDQTPQTLKSGSVDAIAAWQPNS
                     GSALKLVAGSAPIFTSANVPGIIYDELVVSPKSLAERRADWLKVVKVWDRIAKFIKDG
                     ANADEAAKIMSARVGLTPDQYKPLMKGTHFMDLAEAKKHWAKAEGLESVYGSSKIVDD
                     FNLKNKVYKTSMKYEEYLDPSLADEASK"
     gene            2911..>3416
                     /locus_tag="VFQ35_05530"
     CDS             2911..>3416
                     /locus_tag="VFQ35_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930121.1"
                     /translation="MEKVEHSPGATRVGTGRRPRYFAVRKPLSARASFVFGILAFVLP
                     LLSWCVVSYVPFVWHPEVVVVDAGDAKLPGQYNFLTPGERVDRDVFEKRNLELAKAGR
                     PLAKGFRANPIFLPAPHEVGKAFYTAFTTPPARTGDFWLHESLLQSCKIIFWGFVYSA
                     LLGVPLGI"
BASE COUNT          664 a         1063 c         1100 g          589 t
ORIGIN      
        1 ggtgagtccg gtcaggtcga gcttgcgcga cagaaacgcg acttcacttc cccacacttt
       61 gaaagtgcgg gtcgaccgct tcatcgtatc gaggaaccag gcgcgttgct cggcccccaa
      121 gatgttctgc gcctcgggac gctgcgagac cgcgagccga gcgagctcgt agaaggggcg
      181 ctccgcgaga agatatcgtg agccgattcg cccgtactgc gctgacttca agagctgatg
      241 ataagcgtag ccccgttcga gtgttggctc ggtcagctcg attgcgggga gcaacgactc
      301 gactactgcg agctgcgcgt tgagccaggg cgcgctcacg tagcctgcga tttgagacgg
      361 gtcgagaccg agctccgccg cgtgcgtcct gagctcggcg ccgtacgcat cgaagggcgc
      421 gatgtcgacg ttgacacagg ggacgagctc ttcggcggga acgtcgggca gcgccgcgct
      481 caacgcgaag atgacccccg gcgacgcacc ttctggcacc aggtggtccg gtcggtagcg
      541 ccgctcgtcg gtcagcacga gctccaggtg cttgccaaag gcgaagctgc ggtaaatccg
      601 gagatcgtcc ggaaactcga gcgcagactg ccacttcgaa gcggtgttgt cggtgtagtc
      661 gactggcatg tactcgaacc aggcttggtc cgaagccata cgccgctcga catcggtttc
      721 gtcgacgcgc tcgtcgaagt acgtcgcggt cgcaccgtgc gagtcgtcgg agaattcgtg
      781 gtcgtcgggg atgacgatca tggcaaagcg ctcgtgtagc gcctgcagat ccggatccga
      841 gcgatacaac cggtacagat cgcgatagtt gtccagggaa cgcgcggcga gctcccctcc
      901 accgagttcg agtgcctctt ccggcttgct gaacaccacc gcccgaggtc catcggcgct
      961 cgcgcgaccg gtctcgtaga tgtagtctcc gaggtgcagc acgaagtcga gctcgtacgt
     1021 cgcgagctga cggagaacgt gaaagtagcg gccggcgtac gactggcagc agaccacgcc
     1081 gaacttcact tgcacgtcag cgtcggcctt cggagcggtg cgggtgcgac cggtgcggct
     1141 cgtcacggtg ccttcggacc cgatgtaacg gaaccgatag tagtaggtcg tgccggcctc
     1201 gagcgaatcc acgcgcaccg taaggcagtg gtcggagact gcggacgccg tgagctcggc
     1261 ggcagccgca ccattcaggg caatcacctt tcgaaagcct tcgtccttcg atacctcgag
     1321 ctcgacggag agatcttggt cgggccgagc cggatcgacg acgcgcgtcc agagcacgac
     1381 gctagtctcg cgcgggtccc ccgacgccac ggattgcgga aagaattttg cgctcgtgtc
     1441 cgggcgcgaa gcgtcatccg agccacaacc aagcggcgcg cacacggacg acgccgtgac
     1501 cgccgcaaga gagaagaacc gtcgtcgagg caggcgcaga accatcgttg cggcggatta
     1561 caccgcctat cgagcggaat gaaccgcgag tcgcgccacc ttcgaatttt cggggatttt
     1621 ccgtcatcag ccggagccac ccccgcgtct cagcgccggc cttgcggaac gagccagcgg
     1681 gcgtggtcac acccagggag cgcgtgtttt tcggggcgca cagcgccgaa acggggacga
     1741 aagctgcccg caacacgagg cgggagaacg tcggcgtgct tcgcaggctc accgaacgaa
     1801 aggttcatcg catgatcgcg atcgttcggc tcatcggcgt tgctgcgctc gctctgctcg
     1861 gcgttgcgtg caacaaatcc tcctctagcg cgtccggttc ggcggaatcc aagccatcgg
     1921 ctgacgtggc agcggcggca gctcctgctg cttctgcggc cgttcccacg cccccaccgg
     1981 ccccgagcgg cccggctctg cgcatcgcct acagcgattg gccaggttgg gtcgcttggg
     2041 agatcgggat ccagaagggc tggttcaaag aagcgggcgt cgacgtcgac ttcaagtggt
     2101 tcgagtacgt gccctcgatg gaagcgtact ctgccggcaa ggtcgacgcg gtttgcgtta
     2161 cgaacggaga cgcgctcgtc acgggctcga acggcggcaa gagcatcggc atcctgatca
     2221 acgactacag cgacgggaac gacatgatcg tcgcgaagcc gggcatcacg agcgtcgcgc
     2281 aactcaaagg caaaaagatc ggcgtcgagg tcggtttcgt cgaccacctg ctcttgctct
     2341 acgcgctcaa atccgcgaat ctgagcgaaa aagacgtcaa gatcgtcaat gttcccaccg
     2401 accagacgcc gcaaaccctg aagagcggca gcgtcgacgc gatcgcggcg tggcaaccga
     2461 acagcggctc ggcgttgaag ctcgtcgcag gctcggcccc gattttcacg agcgcgaacg
     2521 tgcccggcat catctacgac gagctcgtgg tcagcccgaa gagcttggcc gagagacgcg
     2581 ccgattggct caaggtcgtc aaggtttggg accgcatcgc gaagttcatc aaagatggcg
     2641 cgaacgcgga cgaggcggcg aagataatga gcgcgcgagt cggtctcacc cccgaccaat
     2701 acaagccgct catgaagggc acgcacttca tggatctcgc cgaggccaaa aagcactggg
     2761 ccaaggcgga agggctcgag tccgtgtacg gctcgagcaa gatcgtggac gacttcaatt
     2821 tgaagaacaa ggtctacaaa accagcatga agtacgaaga gtacctcgac ccgtcgctcg
     2881 ccgacgaagc ctccaagtag gatgttgctc ttggaaaagg tcgagcattc gcccggtgcc
     2941 acgcgcgttg gcaccgggcg ccgcccgcgc tacttcgcgg tgaggaagcc gctttcggcg
     3001 cgcgcgagct tcgtattcgg gatcttggcg ttcgtcctac cgctcctttc gtggtgcgtg
     3061 gtgagttacg tccccttcgt gtggcacccc gaagtggtcg tagtagacgc gggtgacgcg
     3121 aagctccccg gccagtacaa ctttctcacc ccaggagagc gcgtcgatcg ggacgtgttc
     3181 gagaaacgaa acctcgagct tgcgaaagcg ggacgcccgc tggcaaaggg attccgcgcg
     3241 aacccaatct tcctgccggc tcctcacgag gtcggcaaag cgttctacac tgcgttcacg
     3301 acgccgccgg cgcgcacggg cgacttctgg ctgcacgaaa gcctactcca gagctgcaag
     3361 atcatcttct ggggcttcgt gtactcggcc ctgctcggtg ttccgctcgg catctt
//
LOCUS       DASTKS010000267         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_43032,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000267 DASTKS010000000
VERSION     DASTKS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43032"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /gene="sppA"
                     /locus_tag="VFQ35_05535"
     CDS             <1..1922
                     /gene="sppA"
                     /locus_tag="VFQ35_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9930122.1"
                     /translation="VLELGLEGRYVTQGPGYWVPRATLGIDIPELGRLHGEFSMSDPA
                     EKVQQREWLGAALMDFGFNGYKGAMELAAGTVFGNALGQSAKGQAHQNLAVDVAFKSY
                     REPYASDTRVHALRIRMESTPSTREHVALLRRLWAIAETEPSVGAVVFELRTAPASSL
                     AHVQELRDAIHQLRVHGKQVVCHLEDADGGALYACSAANRILMNPGGGLRFAGLKARY
                     LYYKTLLENLGVRADFVRIGSHKSAPEAFTRDSASDVAREDKIDLLRQFEWQFTAGVA
                     AGRHIDPLELRKRIAQGPFIADEAKQAGLVDELAFDDEIEASVSKVLGGGIALRDDKR
                     VPWAPKTFGATRGIALVYIDGDMVDGRSKTIPFLGMKLVGSYTIADSIKQARENPMVG
                     AVVLRVESGGGSALAADVIWREVELTTKVKPVIVSMGSAAASAAYYISAPATRIFANP
                     LSITGSIGVFYGKADVAELLRKIGVTVEVYKTAPRADSESIYRPFTPEEHKELEHKVS
                     QFYDQFLGRVAKGRKLSKEAVDAVGQGRVWSGEQALGKHLVDELGGLRQALGEARRLA
                     DLPDYAPIVELPPPDGTLLGKLLGIDGLHEEASERVNAVLPGELLSLARALAPFVVHP
                     ADKPMALIEFTEVQP"
     gene            1919..>2681
                     /locus_tag="VFQ35_05540"
     CDS             1919..>2681
                     /locus_tag="VFQ35_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9930123.1"
                     /translation="MNGGARASLRLDAARIRYENGSLVGPFTFASNAERVALLGDFGA
                     LFRLIANRATLESGTVTLLDVPLQQALSSGVVGAAPLDLPLPPAWTCERYLSESAGLF
                     GLRRRAARERAHTALARFELGALARRTLGSLAVHDRRALAIVRATLNDPKLLLCEAPL
                     SGLPPSAQRLLSVALERTRCALVVSFHELPHFGLERALFETCTQHFVHDRDLAHDRDL
                     VHDRDRDLAHDRDLAHDRDLAHDRDLAHDRDLAHDR"
BASE COUNT          450 a          971 c          842 g          418 t
ORIGIN      
        1 gcgtgctgga gctgggcctc gaggggcgct acgtgacgca agggcccggc tactgggtgc
       61 cccgcgcgac tcttggcatc gatattccgg agctcgggcg cttgcacggc gagttctcga
      121 tgagcgaccc ggccgagaag gtgcagcagc gcgagtggct tggagccgcg ctgatggact
      181 ttgggttcaa cggctacaaa ggcgcgatgg agcttgccgc gggcaccgtg ttcggcaacg
      241 cgctcggtca aagcgccaag ggccaggcgc atcagaacct cgccgtcgac gtggcgttca
      301 agagctaccg cgagccgtac gcctccgata cgcgcgtcca tgcgctgcgt attcgcatgg
      361 agagcacgcc gagcacgcgt gagcatgtcg cgctcttgcg acgcttgtgg gccatcgcgg
      421 agaccgagcc atcggtcggc gcggtcgtct tcgagctgcg caccgctccc gcgagctcac
      481 tcgcgcacgt ccaagagctg cgcgacgcga ttcatcagct gcgcgtgcac ggcaagcaag
      541 tcgtgtgtca cttggaggac gccgacggcg gtgcgctcta cgcgtgttca gccgcgaatc
      601 gcatcttgat gaaccccggg ggcgggctgc gcttcgccgg gctcaaggcg cgctacctct
      661 actacaagac gctcctcgaa aacctcggtg tacgcgccga cttcgtgcgc atcgggtcgc
      721 acaagagcgc ccccgaagcg ttcacgcgag actccgcgag cgacgtcgcg cgcgaggaca
      781 agatcgatct gctgcgccaa ttcgagtggc aattcaccgc cggcgtcgcg gcagggcgac
      841 acatcgatcc actcgagctc cgcaagcgca tcgcgcaagg cccattcatc gcggacgagg
      901 cgaagcaagc gggcctcgtg gacgagctcg ccttcgacga cgaaatcgaa gcctccgtat
      961 cgaaggtttt gggcggcgga atcgcgctgc gcgacgacaa gcgcgtgccc tgggcgccaa
     1021 agacgttcgg ggcgacacgc ggcatcgcgc tcgtctacat cgacggcgac atggtcgatg
     1081 ggcgcagcaa gaccatcccg tttctcggca tgaagctcgt cggctcgtac accatcgcag
     1141 actcgatcaa gcaagcgcga gagaacccga tggtcggcgc agtcgtgctc cgcgtcgaat
     1201 cgggcggagg ctcggcgctc gccgccgacg tcatttggcg cgaggtcgag ctcaccacga
     1261 aggtgaagcc ggtcatcgtg agcatgggct cggccgccgc gagtgccgcg tattacatct
     1321 cggcccccgc gacgcgcatc ttcgcaaacc cgctcagcat cacgggcagc atcggtgtct
     1381 tctacggcaa ggccgacgtc gccgagctcc tgcggaagat cggcgtcacg gtggaggtct
     1441 acaagaccgc gccgcgcgcc gatagcgagt cgatctatcg acccttcacg cccgaagagc
     1501 acaaggagct cgagcacaag gtcagccagt tctacgacca gttcctcgga cgcgtcgcca
     1561 agggccgcaa gctctcgaaa gaagccgtgg acgcggtcgg ccaaggtcgc gtgtggagcg
     1621 gcgagcaggc gctcggcaag cacctggtcg acgaactcgg aggccttcgc caagccctcg
     1681 gcgaagcgcg ccgtctcgcc gatttgcccg actacgcgcc catcgtcgag ctccctccgc
     1741 cggacgggac gctgctcggt aagctcctcg gcatcgatgg cctgcacgaa gaagcgagcg
     1801 aacgagtgaa cgcagtgctc ccgggcgagc tcctttcact cgcgcgcgct ctcgcgccgt
     1861 tcgtcgttca cccggcggac aaaccgatgg cgctcatcga gtttacggaa gtgcagccgt
     1921 gaacggcggc gctcgagctt cacttcggct cgacgctgcg cgcatccgct acgaaaacgg
     1981 ctcgctcgtc gggccgttca ctttcgcgtc caacgccgag cgcgtcgcac tgctcggcga
     2041 cttcggcgcg ctctttcgcc tgatcgcaaa ccgcgccacc ctcgagagcg gcaccgtcac
     2101 gttgctcgac gtcccgctcc aacaagcgct gtcgagcggc gtcgtgggcg ccgctccgct
     2161 cgacttgccc ctcccgcccg cgtggacctg cgagcgctac ctcagcgaga gcgccgggct
     2221 ctttggctta cggcgaaggg ccgcgcgtga gcgcgcccac accgcgctcg cgcgcttcga
     2281 actcggagcg ctcgcccgcc gcacgctggg ctcactcgcc gtacacgacc gccgcgcgct
     2341 cgccatcgtg cgcgccaccc tgaacgaccc caaactcctc ctctgcgaag cgcccctttc
     2401 gggcctgcct ccgagcgcac agcgcctgct cagcgtcgcg ctcgagcgca cccgctgcgc
     2461 cctcgtcgtc tccttccacg aactcccgca cttcggtctc gaacgcgccc tcttcgagac
     2521 ctgcacccaa cacttcgttc acgaccgcga cctcgctcac gaccgcgacc tcgttcacga
     2581 ccgcgaccgc gacctcgctc acgaccgcga cctcgctcac gaccgcgacc tcgctcacga
     2641 ccgcgacctc gctcacgacc gcgacctcgc tcacgaccgc g
//
LOCUS       DASTKS010000268         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1157813, whole genome shotgun sequence.
ACCESSION   DASTKS010000268 DASTKS010000000
VERSION     DASTKS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157813"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFQ35_05545"
     CDS             complement(<1..1064)
                     /locus_tag="VFQ35_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9930124.1"
                     /translation="MLSLRSVTRFLVPMAFGAALLRCTSTPDVAVNHGNGGMAGAPVA
                     GTSSSSGGKTDIEIPMSGGRGGEGGAGAEGGAPNMDAVCGDSELGTGEGCDDGNAIPG
                     DGCSGICKTEPGWDCKDVGQPCVSLVKCGDGEVGGAEACDDGNVAPKDGCSATCTVEP
                     GFRCDDPGKPCTPSQTAECGNGAVEFGETCDDKNVASLDGCDATCQREPGFSCPQPGQ
                     LCVRQEICGDGILTTGEECDDANKLPGDGCDGVCHKEPFYTCPKPGKPCESTIVCGDD
                     KVVGDEACDDGNNVAKDGCSADCKQVEPGFTCPKNAGVGGPCVPVVANTCGDGILSFG
                     EYCDDGNQDPNDGCTATCTVT"
     gene            1606..3303
                     /locus_tag="VFQ35_05550"
     CDS             1606..3303
                     /locus_tag="VFQ35_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930125.1"
                     /translation="MSSVTLAKRLLLATGLLSAAATAALGYGVREAWRRAEEQRFESE
                     FQPAVELLTTELAREFTNLPVSLGSHCAHDSLVDQAQIGLTSGDLESRRLSISLRVPE
                     LAKALEVEELWLVTPRGEVLGAHATSLVGQRDPKLAARVVELGDQARLTPGPGHRIEA
                     ACRRKDPQNPKLWVGLLARRDVDAVLARLGKAYAMTLSLEPVREAPGALTRRFELAGL
                     PGVSVTASRSRLPLTTALSRLDSTVLLIGGLTMLAALAAAGLLSRGLARPFVELAREA
                     SAAMQGEPRPILARGPREIEEAAAAFNRAIQDLLALRGRLAATERIAAWREIARSVAH
                     EIKNPLAPIRAAIETLRRLRARQDPAFDDYFDEATRTALAEVNRINQIVSEFTEFARL
                     PPPSPTEVDIVQLARDVVGLHATGGAAVTLDERACPKVRADQNQIVQVVTNLVQNALD
                     AVSGRPAPEVVVRVAPVEGERVRVSVHDNGPGIAAEMRERLFLPYATTKPHGTGLGLA
                     IAQRLVVEHGGEIGCRDGAHGGTEFWFELPVSGPNPASPPAEGAQPAQKAGTAREVA"
     gene            3413..>4420
                     /locus_tag="VFQ35_05555"
     CDS             3413..>4420
                     /locus_tag="VFQ35_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9930126.1"
                     /translation="MTRLTPRQVLALLALAAVAAITWAAAGLDQHFAVYALVLAFASA
                     AAVVLAGSEGGIAPDALSALRLAARRMGEGKRPQAPAGTGIELLAIYDALGDTFDTIG
                     TRGSQALLDSTQSATTVVRQLSDGISSQLSASEETARFIKEMATAHHDIATNVEMLAA
                     GTEESSSSVLEMNATTDEVTENIGELASGVRETVASIEEMAYSIKEVAKNVDALSLTA
                     EETSSSMNEMDVSIDQVQSNANETARLSEEVAQDAERGAEAILKTIGEIYRIKESSQE
                     AVAVISNLGSRIDAIGQILNVIDDVAEQTNLLALNAAIIAAQAGEHGKGFAVVADEIK
                     DL"
BASE COUNT          759 a         1538 c         1407 g          716 t
ORIGIN      
        1 gtcacggtgc aagtcgcggt gcagccgtcg ttcggatcct ggttcccgtc gtcgcagtac
       61 tcgccgaagc tcaagatgcc gtcgccacag gtgttggcta cgaccggcac gcacgggccg
      121 ccgacgccgg cgttcttcgg acacgtaaag ccgggctcga cctgcttgca atctgcagag
      181 cacccgtctt tggccacgtt gttgccgtcg tcgcaagcct cgtcgccgac gaccttgtcg
      241 tcaccacaaa cgatcgtcga ttcacacggc ttgccaggct ttgggcaggt gtagaacggc
      301 tctttgtggc agacgccatc gcagccgtca cccgggagct tgttggcgtc gtcgcactct
      361 tcccccgtcg tgaggatgcc gtcgccgcag atctcttgcc gcacgcagag ctgccccggc
      421 tgcgggcagc tgaaccccgg ctcgcgctga caggtcgcgt cgcagccgtc gagcgacgcc
      481 acgttcttgt cgtcgcacgt ctcgccgaac tccacggcgc cgttgccgca ctccgccgtt
      541 tgagaaggcg tgcagggctt acccgggtcg tcacaacgga accctggctc gacagtgcac
      601 gtcgccgagc agccgtcttt cggagcgacg ttgccgtcat cgcaagcctc ggcgccgccg
      661 acctcgccgt cgccgcactt tacgagcgag acgcagggct gcccgacgtc tttgcagtcc
      721 cagccgggct ccgtcttgca gataccggag cagccgtcgc cggggatggc gttgccgtcg
      781 tcacagccct cgcccgtgcc gagctcgctg tcgccgcaga cggcatccat gttcggtgcg
      841 ccgccctcag cgcccgcgcc gccttcgccg cctctgccgc ccgacatcgg aatctcgata
      901 tcggttttgc ctccgctgct gctgctggtg cccgcgacgg gcgcgccagc cataccgccg
      961 ttaccgtgat tgacggcaac gtcgggagtg gaggtacagc gcaagagcgc tgcgccgaag
     1021 gccatgggca caagaaagcg agtgaccgaa cgtagagaca acatggaaat ccgtacgcac
     1081 gccgaatggg agaacacccc taagagacca gcgcggagta tgcggcaagc accggtcgag
     1141 ctcaaccacc aagcaggccg tgtcggcgct ttcttcggag cctcgccgcg acggccttgg
     1201 ctcgttactt cgccgtgacc tcgcgtgtat aacctgtggc aagtcgcgtc gcttctagct
     1261 cgcccgcagc gcgacgcgcc caacgaggct cctcgccgta cgcacccccg gggtggcccc
     1321 aacgttaccc tgcgcgcgcg agctcctcga gcggcaaagc acggcggcag cgctcgactc
     1381 cgctcgaaag cttcggcctt tcgccatgct ctttcctatg ggcgtgttct tcgctcgcgt
     1441 ttcttggctc atcgcgacgg gtaaaggttg gagccctatg actcgagcgt ggcgtccccg
     1501 cgcgcggcag gcgctcacgc cacgaggtaa ctcgacttcg caacggcttc tcgagttaca
     1561 ctcgcacgag attcaagccg cgcactcctc cccttctgac gcacgatgtc ttcggtcacg
     1621 ttagccaagc gcctgttgct cgcgacgggg ctcctcagcg ccgccgcgac cgcagcgctc
     1681 gggtacggcg tccgggaagc gtggcgtcgc gccgaggaac aacgtttcga gtctgaattt
     1741 cagcccgcag tggagctttt gaccacggag cttgcccggg agttcacgaa tcttccggtt
     1801 tcgctcgggt cacactgtgc acacgactcg ctcgtggacc aagcacaaat tgggctcact
     1861 tcgggcgatc tggaatcacg acggctgtcc atcagcttgc gcgtccccga actcgcgaag
     1921 gcgctcgagg tcgaggaact ctggctcgtg acgccacgcg gcgaggtgct cggcgcgcac
     1981 gccacgagcc tcgtaggtca gcgcgaccca aagcttgccg cgcgcgtggt cgagctcggc
     2041 gatcaagcgc gcctcactcc ggggcccggc caccgcatcg aagcggcttg tcgacggaaa
     2101 gatccgcaaa accccaagct ctgggtgggc ctgctcgcgc gccgcgacgt cgatgcggta
     2161 ctcgcgcgtc tcggcaaagc gtacgcgatg accctgtcgc tcgaacccgt ccgcgaagca
     2221 ccgggcgcat tgacccgtcg cttcgagctc gccgggctgc ccggcgtcag cgtcaccgcc
     2281 agtcgctccc gtctcccgct gacgaccgcg ctcagtcgcc tcgactcgac cgtcctcctc
     2341 ataggagggc tgacgatgct ggcggcgctg gcggcggcgg gcctgctctc gagaggtcta
     2401 gcgcgtccgt tcgtcgagct cgcgcgcgaa gcgagcgcgg ccatgcaggg cgagccacgg
     2461 ccgatactgg cccgcggtcc gcgcgagatc gaagaagcgg ccgccgcctt caaccgggcg
     2521 attcaggatt tgctggcgct ccgcggaagg ctcgccgcga ccgagcgcat tgccgcctgg
     2581 cgcgagatcg cccggagcgt cgcccacgaa atcaaaaatc ccctcgcgcc catccgcgcc
     2641 gcaatcgaga ctttacgacg gctcagagct cggcaagacc ccgccttcga cgactacttc
     2701 gacgaggcga cgcggaccgc cctcgccgaa gtgaaccgaa tcaaccaaat cgtctccgag
     2761 ttcaccgagt tcgcgcggtt gcccccgccg agcccgaccg aggtggacat cgttcagctc
     2821 gcgcgggacg tcgtcgggct acacgcgacc ggaggagcgg cggtgacgct cgacgaacga
     2881 gcgtgcccca aagtgcgagc ggaccagaac caaatcgttc aagtggtcac gaacctggtc
     2941 cagaacgcgc tcgatgcggt ctccggccga ccggcaccgg aagtcgtcgt tcgcgtcgcg
     3001 ccggtcgaag gcgagcgagt gcgtgtgagt gtgcacgata acggacccgg catcgcggcc
     3061 gagatgcgcg agcgcttgtt cctgccctac gcgacgacga aaccgcatgg aaccggcctc
     3121 gggctcgcga tcgcgcagcg gctcgtcgtc gaacacggcg gcgaaatcgg ttgtcgcgac
     3181 ggagcgcacg gcggcaccga gttctggttc gagctccccg tttccggccc gaaccccgcg
     3241 agcccaccgg ccgaaggcgc tcaacccgcg caaaaggcgg gcaccgcgag agaagtcgct
     3301 taacgaacgc cgctctgcgc cgcttctcgc cacgtcgccc gcacgccgac gtcaacactc
     3361 ttgttcggcg gggcaagggg caagtacgct gaacgtctcg ggaagccatc ggatgacacg
     3421 ccttacgccg cgtcaggtcc tggcactact cgcgttggct gctgttgctg ccatcacgtg
     3481 ggctgctgct ggtctcgacc aacatttcgc cgtttacgct ttggtgctcg cgttcgcgag
     3541 cgctgcggcg gttgtgctcg ctggttccga aggggggatc gcgccggacg cattgtcggc
     3601 gctacgcctc gccgcgcgac ggatgggcga gggcaagcgc cctcaggctc ccgctggaac
     3661 cggcatcgag ctcttggcca tctacgatgc gctcggggac accttcgaca cgatcggcac
     3721 gcgcggttcg caggcgctgc tcgactcgac gcagagcgcg acgacggtgg tgcggcagct
     3781 gagcgacggc atcagctcgc agctcagcgc gagcgaggag acggcgcgct tcatcaaaga
     3841 gatggcgacc gcgcaccacg acatcgccac caacgtcgag atgctcgcgg cgggcaccga
     3901 agagtcgagc tcgtccgtcc tcgagatgaa cgcgaccacc gacgaagtca ccgagaacat
     3961 cggggagctc gcctcgggtg tgcgtgagac ggtcgcctcg atcgaggaga tggcctactc
     4021 gatcaaagaa gtcgcgaaga acgtcgacgc cctctcgctc accgccgagg aaaccagctc
     4081 gagcatgaac gagatggacg tctccatcga ccaggtccag tcgaacgcca acgagacggc
     4141 gcgcctctcc gaagaagtgg cccaagacgc cgagcgcggc gccgaagcca ttctgaagac
     4201 gatcggtgag atttaccgca tcaaggagag cagccaagag gcggtggcgg tgatctcgaa
     4261 cctcggctcg cgcatcgacg cgatcggtca gatcctgaac gtcatcgacg acgtcgccga
     4321 gcagaccaac ctgctcgcgt tgaacgccgc catcatcgcg gctcaggccg gcgagcacgg
     4381 caaaggcttc gcggtcgtcg ccgacgaaat caaagacctc
//
LOCUS       DASTKS010000269         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_264344, whole genome shotgun sequence.
ACCESSION   DASTKS010000269 DASTKS010000000
VERSION     DASTKS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264344"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFQ35_05560"
     CDS             <1..1039
                     /locus_tag="VFQ35_05560"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET9930127.1"
                     /translation="SQTAPDPEETNPRALTEFRTQLDPYGTWQQHPTYGTVWVPSASV
                     VGTGFYPYVSSGRWALDEDGNWIWVSDFSFGRVVFHYGRWVWLGAGWGWIPGYRYAPA
                     WVSWRVPTGSYAYVGWSPLGPSYLWFNGYAVSYWYGAYTPWVYCPSAYVFHRSVNYYV
                     VRDRALVGRLGTQTRLYAPAAPRVGARASMSVVSPSLGAARVPARSVPSTRIAASSQM
                     AGAVPRARADRFSQPSRIDRVRPDFRGGDGPRSRESFRSASPRSSGGDTWGRSSPSRS
                     FDSGSRSRPSFEGSRPSFGGSRPSFGGSRPSFGGSRPSFGGSRPSFGGGGGSRPSFGG
                     GSHGGGGHGRR"
     gene            1085..1642
                     /locus_tag="VFQ35_05565"
     CDS             1085..1642
                     /locus_tag="VFQ35_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930128.1"
                     /translation="MRGSLLGLALCAVTLASGCTYGHHHDDDGYSGGVDCRSATVREG
                     KIDTDALIDVNPGEGVGVFVEYEAGGRWHVYASCDTKVSRQDCLFDVVVHSLGTNGIL
                     SATGDGLEDDDNVSLFGVDGVQMVTYTDYDFDGFFVQTDPGATLSLDVLLDDGCGEQW
                     LYWIDQGGVHEGAPSTPFELTPSSP"
     gene            1639..>2894
                     /locus_tag="VFQ35_05570"
     CDS             1639..>2894
                     /locus_tag="VFQ35_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930129.1"
                     /translation="MSLSPVAPLTGGVRRFSNSVQRFGRDVRRWFASLRGDPTAQRRL
                     VFELAFALAALSLAWPLFVARYVPIQDLPQHAAAVRVLHDYRSPAFGFERWFEIHWAS
                     TQYVAVYFAAHLLAYLFEPVIATKLVLAASLVALPYALRALLQALRKPASYALLVVPL
                     VYNTQLVLGFLNFVFGLPLMFAGLALFVRYQEHPTRARLVALSLVTTLCFLSHVVPYG
                     MLVLGALALSAGPLSTRPRSPGPQSAGPQSTRPQSALALSAGPQSAGPPVALPLPAAP
                     TATRRLNVPLAARRESANALHLALPFAPSLLLALIWSASTPAGKAFSAISTSGTLFAS
                     REPFSTRLAEIPFWLNDILWSHEDEHALFGALALFLLIGLAGLAGLAGLVGSRAVSPA
                     APEPAPAAAREPVPGAAPAALGPSRAV"
BASE COUNT          362 a         1030 c          922 g          580 t
ORIGIN      
        1 ttcgcaaacg gcgcccgacc ccgaggagac caatccgcgc gcgttgaccg agtttcgcac
       61 ccagctcgat ccgtatggca cttggcagca acacccgacc tacggcaccg tttgggttcc
      121 gagcgcgtcg gtggtgggca ctggcttcta cccgtacgtg agctcgggtc gctgggcgct
      181 cgacgaagac gggaattgga tctgggttag tgacttctcg ttcgggcgcg tcgtgttcca
      241 ctacggtcgc tgggtttggc tcggcgcagg ctggggctgg atcccgggct atcgctacgc
      301 tccggcgtgg gtgagctggc gagtccccac gggctcttat gcctacgtgg gttggtcgcc
      361 gctcggtccg agctacttgt ggttcaacgg gtacgccgtt tcctactggt atggcgcgta
      421 cacgccgtgg gtctattgcc cgagcgcgta cgtgttccac cgctccgtga actactacgt
      481 cgtgcgagat cgcgcgttgg tgggccggct cggtactcag actcggctct acgcgccggc
      541 cgcaccgcgc gtcggtgcgc gcgcgtcgat gtcggtcgtg agcccttcgc tcggcgctgc
      601 acgcgtgccg gcgcgctcgg tgccgtcgac gcgcatcgcg gcttcctccc aaatggcggg
      661 cgccgtgcct cgggctcgcg ccgatcgctt ctcgcaacca tcgcgtatcg accgggtccg
      721 ccctgatttt cgtggcgggg atggtccgcg ttcgcgcgag agcttccgct cggcctcccc
      781 ccgctcgagc ggcggagaca cttggggccg ttcgtcgccc tctcgctcgt tcgacagcgg
      841 gtcgcgctcg cgtccgagct tcgagggttc tcgcccgagt tttggcggct cgcggcccag
      901 cttcgggggt tctcgcccga gcttcggcgg gtcgaggcca agctttggcg gttcacggcc
      961 gagcttcggg ggcggcggag gttcgcgccc gagctttggc ggcggttcgc atggcggtgg
     1021 tggtcacggt cggcggtgat cacgggtgtt gagggttgaa cggaattcgg agaggattcg
     1081 acagatgcgc ggttcgctct tgggcttggc tttgtgtgcg gtgacgttgg cttctggctg
     1141 cacttacggt caccaccacg acgacgacgg ttactcgggc ggtgtcgact gccgcagcgc
     1201 gacagtgcgc gaaggcaaga tcgatacgga cgctctcatc gacgtcaatc cgggcgaagg
     1261 cgtcggagtc ttcgtcgagt acgaggccgg cgggcgctgg cacgtgtacg cctcgtgcga
     1321 cacgaaggtc tctcggcaag actgcttgtt cgacgtcgtg gttcactcgc tcgggacgaa
     1381 cggcattctc tctgccactg gcgacgggct cgaagacgac gacaacgtct ccctgttcgg
     1441 agtcgatggc gttcagatgg tgacctacac cgattacgac ttcgacggct tcttcgtcca
     1501 aacggatccg ggcgcaactc tgagcctcga tgtgttgctc gacgacggct gcggtgagca
     1561 gtggctttac tggatcgacc agggcggcgt gcatgaaggc gcgccctcga cgcctttcga
     1621 gctgactccg agcagtccgt gagcctttct cccgtagcgc cgctcactgg cggcgtacgg
     1681 cgattcagca acagcgtaca gcgattcggg cgcgatgtac ggcggtggtt cgcgtccttg
     1741 cgcggagacc ccaccgcgca gcgccgcctc gttttcgagc tcgccttcgc cttggcggcc
     1801 ctgtcgctcg cctggccgct cttcgtcgcg cgctacgtac ccattcaaga cctgccgcaa
     1861 cacgcggcgg cggtgcgcgt cctgcatgac taccggtcgc ctgccttcgg cttcgagcgc
     1921 tggttcgaga tccattgggc ttcgacgcag tacgtcgccg tctacttcgc ggcgcacctc
     1981 ctcgcgtacc tgttcgagcc cgtcatcgcg accaaactcg tcctcgcggc gtcgctcgtg
     2041 gcgctgccct acgcgctgcg cgccttgctc caagccctcc gcaaaccggc gagctacgca
     2101 ttgctcgtcg ttccgctcgt ctacaacacc cagctcgtcc tcggcttctt gaacttcgtc
     2161 ttcggactgc ccctgatgtt cgccgggctc gcgctcttcg tgcgctacca agaacacccg
     2221 acccgcgccc gcctcgtcgc cctgtcactc gtcacgaccc tgtgcttcct ctcgcacgtc
     2281 gtgccgtatg gcatgctcgt gctcggcgca ctcgccctga gcgcaggccc gctgagcaca
     2341 cgcccgcgaa gcccaggccc gcagagcgca ggcccgcaga gcacacgccc gcagagcgca
     2401 ctcgcgctga gtgcaggccc gcagagcgca ggcccgcccg tggcgctccc gttgcctgca
     2461 gcgccgaccg ccactcgtcg cttgaacgtg ccgctcgccg ctcgccgcga atcggccaat
     2521 gcgcttcacc tcgcactgcc cttcgcaccg tcgctcttac tcgccctcat ctggagcgcg
     2581 tcgacgcccg ctggcaaagc cttctccgcg atctccacga gcggcaccct cttcgcctcg
     2641 cgcgaaccct tcagtactcg cctcgcggaa atccccttct ggctgaacga catattgtgg
     2701 tcgcacgaag acgaacacgc cctcttcggc gcactcgcgc tctttctcct catcggcctc
     2761 gcgggcctcg cgggcctcgc cggcctcgtc ggctctcgcg ccgtttcgcc cgctgcgccg
     2821 gagcccgccc ctgccgctgc gcgcgagcct gttcctggcg ctgctcccgc tgccctcggg
     2881 ccttcccgcg ccgt
//
LOCUS       DASTKS010000270         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_43305,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000270 DASTKS010000000
VERSION     DASTKS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43305"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFQ35_05575"
     CDS             complement(<1..468)
                     /locus_tag="VFQ35_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9930130.1"
                     /translation="MANVAIEVLSGGASGRVVRLDADVIRIGRSAGAEVELPEPHVSA
                     DHARLLVGSEQVVLEDLDSTNGTWVVRGGARIRVGEGASRLIVQDGDELELGGEGEHG
                     TRLRLGLSPDRETGQLLAVRPVAEVAKSAAALDEAPRLRALLAELAHIGAADEL"
     gene            complement(547..2265)
                     /locus_tag="VFQ35_05580"
     CDS             complement(547..2265)
                     /locus_tag="VFQ35_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930131.1"
                     /translation="MLGSATPAVVVLTALSATGCKDESQPEYWVDKLNEPTWRARAVN
                     RFEQFFEDSLTRANKNLKAPEVQELINKSVEPLTKAYVEGGDQLDPKSRVALIKLLSG
                     YRDKRADPAFKKAFEDFAKSPKTNKDDTDIKWAAIAVGDMKEEALAQPMLEAFLKLRA
                     NTMLGGVVYKDYNAAMLQMPNKAWAGPLMQKLEAEVSRPGPQQKKDKDAIEEYMDQQF
                     WQTTAAALLGKIGDPAAVEPLIKVILDPAKIDFQATAMVALIQLGKPATERAIKLLKG
                     QDEKLESFCLRRIKDTGGDVKGKPCVQSAAVILGSIGRADAVGPMIEVLKSDVDESTK
                     ALIARELPKLPATPESIAAFKSVYESLGLGAQIPPGQPALESLTEAAGQFFDPSLIDW
                     LLKRADTIKAGADEKDEKQGLQQAITVTAIKLAKPDQLATVKRAASQYGTSLEKDLLA
                     QAEGLLKACEDRAACYVEAITKRENQDRKTQFTGIKAGYMAAIYGNEQTRDELVNKLE
                     SIDNAAVRYVSSLAIDRLSPKGSKSAVDKMGAIIDKNAKSPDRDKAAGDAPLKQVMYR
                     LSARGG"
     gene            complement(2620..>3559)
                     /locus_tag="VFQ35_05585"
     CDS             complement(2620..>3559)
                     /locus_tag="VFQ35_05585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9930132.1"
                     /translation="EFRGFARKHRINTWFRQGGYLFLARNEARAAELERSVSVQRRCG
                     LDTELLPPQRIRDISPEVSDEGVLVASYNAHDAVVFPWPFVWGYAEGARALGVEIETF
                     TRVVGIDVENGSVRRVHTDRGSIEAEVVVVACGALSPEVARMAGVELPTHPHRHEICS
                     TEPLKPFLTPLVADLTDGLYFSQSTRGEIVGGISDPKAPTEPRQDSSARFLALYARAL
                     VGRCPRLGAVRVLRQWAGLYDISPDQGPILGAAESPAGLYLASGFMGHGFMMAPVVGK
                     RLAALIAHGTSSAELAAWRLARFREGRTLDGEMIIG"
BASE COUNT          544 a         1168 c         1181 g          666 t
ORIGIN      
        1 gagctcgtcg gcggcgccga tgtgcgcgag ctctgcgagg agcgcgcgga ggcgtggagc
       61 ctcgtcgagc gccgcagcgc tcttcgcgac ttcggcgacg ggccttacgg cgaggagctg
      121 gcccgtctcg cggtcgggcg atagccccag tcgtagccgg gtcccgtgct caccttcgcc
      181 cccgagctcg agctcgtcgc cgtcttgcac gatgaggcgg ctcgcgcctt cacccactcg
      241 aattcgcgcg ccgccgcgga cgacccaggt cccgttcgtg ctgtcgagat cttcgagcac
      301 gacttgctca ctgccgacca agagccgcgc atggtcggcg gagacgtgcg gctcgggcaa
      361 ctcgacttcg gcgccggcgc tcctgccgat gcggatcacg tccgcgtcca agcgcacgac
      421 gcgcccactg gctcccccgc tcaggacttc gatcgcaacg ttggccatcc cagtcgtgag
      481 catagctcgc gttcagcccc gggcgaggcg cacggggccg aacacgctgc tctgtccgag
      541 tttggctcaa ccgccgcggg cggagaggcg gtacatgacc tgcttcaacg gcgcgtcgcc
      601 ggcggccttg tcgcgatccg gcgacttcgc gttcttgtcg atgatggcgc ccatcttgtc
      661 gaccgccgat ttcgagccct tcggcgagag gcgatcgatg gcgagcgagg agacgtagcg
      721 aacggccgcg ttgtcgatgc tctcgagctt gttgacgagc tcgtcgcgcg tctgctcgtt
      781 gccgtagatc gcggccatgt agcccgcttt gatcccggtg aactgcgtct tgcggtcttg
      841 gttttcgcgc ttggtgatcg cctcgacgta gcaggcagcg cgatcttcac acgccttcaa
      901 gaggccctcg gcttgcgcca agagatcttt ctcgagcgac gtgccgtact gcgaagccgc
      961 gcgcttcacg gtggcgagct gatctggctt ggcgagcttg atcgcggtca ccgtgatcgc
     1021 ttgctggagc ccttgcttct cgtccttctc gtcggcgccg gccttgatgg tgtccgcgcg
     1081 cttgaggagc cagtcgatga ggctcggatc gaagaattgg ccggcggctt cggtcaacga
     1141 ctcgagcgcg ggttggcccg gcgggatctg agcgccgaga ccgaggctct cgtaaaccga
     1201 cttgaacgcc gcgatcgact cgggcgtcgc cggcagcttc ggtagctcgc gcgcgatgag
     1261 cgccttcgtc gattcgtcga cgtcgctctt cagcacctcg atcatcgggc ccaccgcgtc
     1321 cgcgcgccca atcgaaccga ggatcacggc cgcgctctgc acgcagggct tgcccttcac
     1381 gtcgccgccc gtgtccttga tgcgacgcag acagaacgac tcgagcttct cgtcttggcc
     1441 cttcaggagc ttgatcgcgc gctcggtggc gggctttccg agctggatga gcgcgaccat
     1501 ggccgtcgct tggaagtcga tcttggcggg atccaagatg accttgatga gcggctcgac
     1561 cgcagccgga tcgccgatct tgccgagcag cgcggcggcg gtcgtctgcc agaactgctg
     1621 atccatgtac tcttcgatcg cgtccttgtc cttcttctgc tggggaccag gacggctgac
     1681 ctcggcctcg agcttctgca tgagcgggcc ggcccaagct ttgttgggca tttgcagcat
     1741 cgcggcgttg tagtccttgt agacgacgcc gccgagcatg gtgttggcgc gcagcttgag
     1801 gaacgcctcg agcatcggtt gggcgagcgc ctcttccttc atgtcgccga cggcgatcgc
     1861 cgcccacttg atgtcggtgt cgtccttgtt ggtcttcggg ctcttcgcga agtcttcgaa
     1921 ggccttcttg aaagcaggat cggctcgctt gtcacggtag ccggagagca gcttgatgag
     1981 cgcgacgcgg ctcttcggat ccagctggtc accgccctcg acgtacgcct tcgtgagcgg
     2041 ctcgacgctc ttgttgatga gctcctgaac ctcgggcgcc ttcaagttct tgttggcgcg
     2101 ggtgagcgag tcttcgaaga actgctcgaa gcgattgacg gcgcgcgctc gccaagtggg
     2161 ctcgttcagc ttatcgaccc agtactccgg ctgactctcg tctttgcatc cggtcgccga
     2221 aagggcggtc agaacgacga cagcgggcgt tgcagatccg agcatgagtg cgccgatcaa
     2281 ggacgcaact cttcgtgaag agcgcagcat tagagagtcc tcccaggcga atgacgaaga
     2341 cgacgacagg gcgagagaag ctcggctcga cggtccgcat ctcggtaccg cgaatgagat
     2401 ttcccgtcgc agcggaacgt aagccgcgcg catcctcgct gtcaacgaat gcgagaaagc
     2461 tgctgcgtcg cgtcacccgt tacgcacgcg tttgcccgcc gatgatgtgt ctcgcgcgcc
     2521 ccacaccacc gggcttgtcc accgggcttg cgcccgggcg ggccgactgt ttgtgcgcgg
     2581 tccaccgggc ttgcgcccgg gcgggccgac cggccaccgt tacccgatga tcatttcgcc
     2641 gtccaaggtg cggccttcgc ggaagcgagc gagtcgccac gcggcgagct ctgcgctcga
     2701 ggttccgtgc gcgatcagcg cggcgagtcg cttgccgacc acgggcgcca tcatgaagcc
     2761 gtggcccatg aatccactcg ctaagtagag ccccgcggga gattcggcgg cacccaagat
     2821 tggtccctga tcgggagaga tgtcgtagag gccggcccac tgccgcaaga cgcgcacggc
     2881 tccgaggcga gggcagcgtc cgacgagagc ccgagcgtac agcgccaaga agcgcgccga
     2941 cgagtcttgg cgcggctcgg ttggcgcttt cggatccgag atgccgccga cgatctctcc
     3001 gcgcgtcgac tgcgagaagt agagcccatc ggtgaggtcc gcgacgagcg gcgtgaggaa
     3061 tggcttgagc ggctcggtcg agcagatctc gtggcgatgc gggtgagtcg ggagctcgac
     3121 gccggccatt cgcgcgacct ccgggctcag tgcgccgcac gcgacgacga cgacttcggc
     3181 ctcgatgctg ccgcgatccg tgtgcacgcg acgaacgctg ccgttttcga cgtcgatgcc
     3241 gaccacgcgc gtgaacgtct cgatttcgac gccgagcgca cgcgctcctt cggcgtaacc
     3301 ccacacgaac ggccacggaa acacgaccgc gtcgtgcgcg ttgtaactcg cgacgagcac
     3361 gccctcgtcc gaaacctcgg gggagatgtc gcggatgcgt tgtggcggga gtaactccgt
     3421 atcgagtccg cagcggcgtt gcaccgagac gctgcgctcg agttcggccg cgcgcgcctc
     3481 gttccgagcg aggaacaggt agccgccctg acgaaaccac gtgttgattc ggtgcttccg
     3541 cgcgaagccg cggaactct
//
LOCUS       DASTKS010000271         5795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_489834, whole genome shotgun sequence.
ACCESSION   DASTKS010000271 DASTKS010000000
VERSION     DASTKS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489834"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFQ35_05590"
     CDS             complement(<1..255)
                     /locus_tag="VFQ35_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930133.1"
                     /translation="MRAQQCRGRSQSLERKIEIPFHTLYDNVSTLAEMGASALCLAVT
                     SEPARHRREETPRNENPERTSAKAQCLDVALGVLSPCHSVK"
     gene            190..1956
                     /locus_tag="VFQ35_05595"
     CDS             190..1956
                     /locus_tag="VFQ35_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930134.1"
                     /translation="MKRNFDLSLEALRATSALLRAHPRVTADQFQRFARELMSRHTSL
                     AALEWAPLVPYAEQKTFEAARTLETGRPFSIREPGEAGAMHAVPARDRYAPLVFLEPF
                     LLDLEGLDIRFETTRRAAVDTAVTSRHLFATAKFRLVEDPPGVFSVAVYDPVPASSDA
                     SAPIAGVAIALYRLDPLVRGALSGTDLRTVDFALLDDTETDPKERILFASRAGAESPP
                     DTHTVYRKQFGFVGRQWSAICATHLEVPWGTALLVFGAGAAVSALVAWLLAAREGRRR
                     LELALRAEHELGGYVLKKQLGAGGMGSVFEATHRLLRRRVAIKLVSPEQNLPELTERF
                     NREARTMADLSSPHTITLYDYGAASDGRLYVVMEYLDGMTLETLVSKFGAQKPERVRH
                     LLLEVCDSLIEAHEAGIIHRDIKPANIMLCKVGGRHDFVKVLDFGLAKSTLSTETKLS
                     RSGIVLGTFTHLAPECLGDSAAASARSDLYSLGCVAYELLSGREVFASVDQTALISAH
                     LTRAPEPLDPALVPAEFERIVLKCLEKDAARRFQSARQLARALKNIDLPAWTEADAAE
                     WWHAFGQSASDQRSQNAAGATA"
     gene            2166..2549
                     /locus_tag="VFQ35_05600"
     CDS             2166..2549
                     /locus_tag="VFQ35_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930135.1"
                     /translation="MSSLRFLASPKSLVTAVVLCLAAPLAAQPRLELERAETVKQLNE
                     AKERVKLLEARLRLLDARRVHEQAAAVQPISNNCSNPFFLDTDGVKHFRVECLVTDTR
                     AACEANPFTVDDDGIKRIRPTCGGQ"
     gene            2837..3205
                     /locus_tag="VFQ35_05605"
     CDS             2837..3205
                     /locus_tag="VFQ35_05605"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET9930136.1"
                     /translation="MAARRNTGSSKKTRKTTRKTSSRKTGRKTSARKTSSRKKSSRRY
                     GSKASKKVGSSMHEMKEGTLRSGRSGRRVTNPKQAIAIGLSEARREGGKVPPAPKKKR
                     SKKGGRKTGSRSSSRSSRSE"
     gene            complement(3262..4056)
                     /locus_tag="VFQ35_05610"
     CDS             complement(3262..4056)
                     /locus_tag="VFQ35_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930137.1"
                     /translation="MERKRLLVLGSGYTGVEVARRGVAEGLTVFASVRRPEQVADIER
                     LGATPLLREALDSSLAEYVDPSTHVVVTFPPDGATDERIAPALARAGAITYVSTTSVY
                     GNITGHIDDDTPVPAPTEQTRPRLLAEATYRNLGGTVLRCPGIYGATRGLHRRVLRGE
                     YSMPGDGSAISSRIHVYDLASFILASTATRAETFVVGDLQPATQREVVGWICATHGLP
                     LPGSAPIAGVSRTLKANRAIDSERARRLLGVALRYPTFREGMSERA"
     gene            complement(4069..4473)
                     /locus_tag="VFQ35_05615"
     CDS             complement(4069..4473)
                     /locus_tag="VFQ35_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9930138.1"
                     /translation="MALPIPVLGLAHVGIRVHDLERSLRFYALFGFEKVLGPLGPEPV
                     AIIRHPCGVEINLVLNAPDPNAPNVLMDIPQKHPGITHISLMCPDIRAAARSLEAAGI
                     ALSGGPVQFTEDSEGIFVRDPDRNVIELHQRH"
     gene            complement(4560..>5795)
                     /locus_tag="VFQ35_05620"
     CDS             complement(4560..>5795)
                     /locus_tag="VFQ35_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9930139.1"
                     /translation="FRALQGLGGGAIIPTAQSILFARYPQKEHGMAAALFGLGAITGP
                     LFGPTIGGYLIEWSSWHWIFMVNVPMGAIATLLTLRFIEEPGFVRPSGAAAKIDVFGI
                     ALLATGMASLQYVLEEGNREGWFDSRLVLALSAVAAIALITFIVHELETEHPVVDLRV
                     FKNTSYAAGTGLNFLTGFALFSGSYMFSLFAGSVIHYSALDIGKVFLVAGTVSVVVMP
                     VMGRLAPRVDGRILLGIGVTVVALSQYVASHLTQAAGFWDLVSPNMIRSFGLGFIFIP
                     VSVLALSSLSPSQRGNATGLFNLTRELGGSLGTALMGMLVSDDIKRFGSYLGENLSPT
                     NPILQEQMGAIASSVGAQTAQRDLVAESVLSMRVMRQAMVQSFENGFRWTAFAMSLGL
                     ILLLFLRRPRNAGPVAGAH"
BASE COUNT         1081 a         1880 c         1873 g          961 t
ORIGIN      
        1 tttgacgcta tggcaaggcg acagcacccc cagagctaca tcgagacact gcgctttcgc
       61 ggaggttcgc tctgggtttt cgtttcgggg tgtttcctcg cggcgatggc gggctggttc
      121 gctcgtcacc gcgagacaga gagcgctcgc gcccatttcc gccagcgtgc tgacgttgtc
      181 gtacaaggtg tgaaacggaa tttcgatctt tcgctcgagg ctttgcgagc gacctctgca
      241 ttgctgcgcg cgcatcctcg cgtcaccgca gaccaattcc agcgcttcgc gcgtgagctc
      301 atgagtcggc acacgagcct tgcagcgttg gagtgggcgc cgctcgttcc gtacgccgag
      361 caaaaaacct tcgaagcggc tcgcaccctc gagacggggc ggcccttctc catccgggaa
      421 ccgggcgagg ccggagcgat gcacgcagtg ccagcgcgcg accgttacgc acccttggtc
      481 tttctcgagc ctttcttgct cgatctcgag gggctcgata ttcgtttcga gacaacgcgc
      541 cgcgctgcgg tcgatacggc cgtcacgtca cggcacctgt tcgcgacggc aaagtttcgt
      601 ttggtagagg atccgccggg tgtcttttca gtcgctgtct acgaccccgt tccagcatcg
      661 agtgacgcgt cggcgcccat cgccggcgtt gcgatcgcgc tctaccggct cgacccgttg
      721 gtgcgcggag ccttgagcgg caccgatctt cgcacggtcg acttcgcgct gctcgacgac
      781 accgagacgg acccgaaaga gcgcatcctg ttcgcaagtc gcgcgggcgc cgagtcgccg
      841 cccgacacgc ataccgtcta tcgcaagcag ttcggcttcg tcggtcgtca gtggtcggcg
      901 atatgcgcca cgcatctcga ggtgccctgg ggaacggctc tcttggtctt cggggcaggc
      961 gcggcggtca gcgctctcgt cgcgtggctc ttggcggcgc gcgaagggcg ccggcgcttg
     1021 gagctcgcgt tgcgcgccga gcacgagctc ggcggctacg tactcaagaa gcagctcggt
     1081 gcgggcggaa tggggagcgt gttcgaggca acgcatcgac tgctgcgccg gcgcgtcgcc
     1141 atcaagctag tgagccccga acagaatttg cccgaattga ccgagcgctt caatcgagaa
     1201 gcgcggacca tggccgatct gagcagtccc cacacgatca cgctctacga ctacggcgcg
     1261 gcgagcgacg gcaggctcta cgtcgtcatg gagtacctcg acggaatgac gctcgagacg
     1321 ctggtatcca aattcggcgc gcaaaagccc gagcgcgtgc gacacctcct gctagaggtc
     1381 tgcgattcgc tcatcgaggc gcacgaggcg ggcatcatcc atcgcgacat caagcccgcc
     1441 aacatcatgt tgtgcaaggt cggcgggcga cacgacttcg tgaaggtgct cgacttcggg
     1501 ctcgcgaagt cgaccctcag caccgagacc aagctctcgc gctccggcat cgtgctcggc
     1561 acgttcacgc atctcgcacc cgagtgcttg ggcgactctg ccgcggcgag cgcacgctcg
     1621 gatctctatt cactcggctg cgtagcgtac gagctcctga gcggacgtga ggtcttcgct
     1681 tcggtcgacc agaccgcgct catctccgcg cacttgacgc gcgctccgga gccgctcgac
     1741 cccgcgctcg tgccagccga gttcgagcgc atcgtgctca agtgtctgga aaaggatgcc
     1801 gctcgccgct tccagtcggc gcgccagctc gcccgcgccc tgaagaacat cgacttgccg
     1861 gcctggaccg aagccgacgc ggccgagtgg tggcatgcct tcggccaaag cgcgtccgac
     1921 cagcgctcgc agaatgccgc gggcgcgacc gcctgagacg cagtcatgcc agaacgacgc
     1981 cggggcgagc gagcccggag aaagcgcacc tgcgcgtact cggcgccatc gcccgcggga
     2041 cgtctcatgt tggagcttcc gtgatgagtg cggtcggaag atgtcgtaca cgctgcgcgc
     2101 gatcaccact gcaagattgt gcgagcaacc agatcacccg ggcgctaaag tttagaaagt
     2161 ccgccatgtc ctcactgcgc tttctggcct cacccaagtc tctggtaacc gccgtcgtgc
     2221 tctgcttggc ggccccgctc gctgcgcaac cgcgcctcga gttggagcgc gccgagaccg
     2281 tgaagcaact gaacgaagcg aaagagcgag tgaagctgct cgaagcccga ttgcggctcc
     2341 tcgacgcgcg ccgagtgcac gagcaagcag cagcggtgca gcccatctcc aacaattgct
     2401 cgaacccctt cttcctcgac acggacgggg tgaagcactt tcgagtggag tgcctcgtca
     2461 cggatacgcg cgccgcgtgc gaagcgaacc cgttcacggt cgacgacgac ggcatcaaac
     2521 gcattcgtcc tacgtgcggc ggacaatagc gaacgtcgcg tcgacgcgcg cagggctgac
     2581 tcgctcgcgc aattgcgcca cgccgcaaag ggctcggagc ctttcctcgg cgagcgcggc
     2641 tgacgccttc gattgaagtg gcgagcagcg tcacgaagac gcactcgtga gtcgcgatag
     2701 gcggctgctg cgcggcagtt cagccattag ttggagctcc accggcgcag cggtaaggcc
     2761 ccgctaacga gtgctcgcga cctcgatggc gcgaacgttt ccgcaaaagg cacgcttgtt
     2821 gctgtggcgt gcgatcatgg cagctcgcag aaacacagga tcttcaaaga agactcgcaa
     2881 gaccactcgc aagacctcga gtcgcaagac ggggcgtaaa acctccgcgc gcaagacgtc
     2941 ttcgcgaaag aagagctctc gccgctacgg ctcgaaggcc tccaagaagg tcggcagcag
     3001 catgcacgag atgaaagagg gaacgctgcg ctcgggtcgc tcgggccgtc gcgtcacgaa
     3061 tccgaagcag gcgatcgcaa ttggcttgag cgaagcgcga cgcgaaggcg gaaaggtccc
     3121 tcccgcaccc aagaagaagc gcagcaagaa gggcggccgc aagacgggga gccgtagctc
     3181 gagccgttcc tcgcgctcgg agtgagctcg gtcgagtcgg tgggtggcgc ggttcgggtc
     3241 gcgtcgccca ccaagctccg ctcaggctcg ctcggacatg ccttcgcgaa acgtcgggta
     3301 gcgcagggca acgcccaata ggcggcgcgc acgctccgaa tcaatcgcgc gattcgcctt
     3361 gagcgtccgc gagacgcccg cgattggtgc ggagccgggc agaggcaagc cgtgggtagc
     3421 gcaaatccat ccaaccactt cgcgctgcgt cgcaggttgc agatctccga ctacgaaagt
     3481 ctccgcccgc gtcgcggtac tcgccaggat gaagcttgcg agatcgtaga cgtgaatacg
     3541 cgatgaaatc gcgctgccat cgcccggcat ggaatattcc ccgcgcaaca ctcttcggtg
     3601 caggccgcgg gttgctccgt agatgcccgg acagcgcagc accgtgccgc caaggttgcg
     3661 gtacgtagcc tcggccagaa gacggggccg cgtctgctcc gttggcgcgg gcacgggtgt
     3721 gtcgtcgtcg atgtgaccgg tgatgttccc gtaaacgctc gtcgtcgaga cgtacgtaat
     3781 cgcgcctgcg cgcgccagcg ccggggcgat acgctcgtcg gtggcgccgt cgggcggaaa
     3841 cgtgaccacg acgtgggttg acggatccac gtactccgcg agggaggagt cgagcgcttc
     3901 gcgaagcagc ggggtcgccc ccaggcgctc gatgtccgca acctgctcgg gtcggcgaac
     3961 gctggcgaac acggtgagcc cttcggctac ccctcggcgc gcgacctcca ctcccgtgta
     4021 accgcttccg agtacgagta agcgcttccg ctccatccgg ttcgagcctc aatgccgttg
     4081 gtggagctcg atgacgttgc gatccgggtc gcgcacgaag atgccctccg agtcctcggt
     4141 gaactgcacc gggccaccgc tcagcgcaat cccagcggcc tcgagtgagc gcgccgcggc
     4201 gcggatgtca ggacacatga gagagatgtg cgttatccca ggatgctttt gggggatgtc
     4261 cattagtacg ttcggcgcgt tggggtcggg agcgttcaga accaggttga tttcgactcc
     4321 gcatgggtga cggatgatcg cgacgggctc gggcccgagc gggcccagca ccttttcgaa
     4381 gccgaacagg gcgtaaaagc gcaggctccg ttccaaatcg tgcacgcgaa tgccgacgtg
     4441 agccaagccg agaacgggga tcggaagggc catgcacagc gcctaccaga tccccctcga
     4501 cgtatcaaag cgagctcggg gccagcccgc cccctcccgt cctttggatt gggctttact
     4561 caatgcgcgc cggcgacggg cccggcgttt cgtggacgcc tcaggaaaag cagcaagatg
     4621 agcccgagtg acatggcaaa cgcggtccag cggaagccgt tctcgaagct ttggaccatc
     4681 gcctgccgca tgacacgcat gctgagcacg ctctccgcga ccaagtcgcg ctgcgccgtc
     4741 tgcgcaccga ccgaactggc gatcgcgccc atctgctctt ggagaatggg gttggttggg
     4801 ctcaagttct cgcccaggta ggagccgaag cgcttgatgt cgtcgctcac cagcatgccc
     4861 atcagcgccg ttccgagcga accaccgagc tcgcgcgtca ggttgaagag gccggtcgcg
     4921 ttgcctcgtt gagaggggga gaggctcgac agcgcgagca cgctcacggg aatgaaaatg
     4981 aagccgaggc cgaagctgcg gatcatgttc gggctgacga gatcccaaaa gcccgccgcc
     5041 tgcgtcaggt gggaggccac gtattgactc aaagcaacga ccgtgacccc gattccgagc
     5101 aagatccggc cgtcgacgcg cggtgcgagc cgacccatga ccggcatgac gaccaccgac
     5161 acggtcccgg cgaccaggaa aaccttgccg atatcgagcg cgctatagtg aatgacggac
     5221 cctgcgaaga gcgagaacat gtacgagccg ctgaacagcg cgaaacccgt caagaagttc
     5281 agccccgtac cggcagcgta ggaggtgttc ttgaaaaccc gaagatcgac cacggggtgc
     5341 tcggtttcga gctcgtgcac gatgaaggtg atgagcgcga tggccgcgac ggcgctcagc
     5401 gcgagcacca agcgactatc gaaccagccc tcgcgattcc cttcctcgag cacgtactgc
     5461 agcgaggcca tcccggtcgc gagcagcgcg atgccgaaca cgtcgatctt cgcggccgcg
     5521 ccgctcgggc gaacgaagcc tggctcctcg atgaagcgca gcgtgagcag cgtggcaatc
     5581 gcgcccatcg ggacgttcac catgaagatc cagtgccaac tcgaccactc gatgagatag
     5641 ccgccgatgg tgggaccaaa cagcggcccc gtgatcgcgc cgaggccgaa gagcgccgcc
     5701 gccatcccgt gctctttctg cggatagcga gcgaaaagga tgctctgcgc ggtcgggatg
     5761 atggcgccgc cgcccaaccc ttgaagcgcc cggaa
//
LOCUS       DASTKS010000272         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_43896,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000272 DASTKS010000000
VERSION     DASTKS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43896"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFQ35_05625"
     CDS             <1..537
                     /locus_tag="VFQ35_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930140.1"
                     /translation="FAPGARASYIEKPRMDPQRMPNERSRLGRDVYLALAAVGWADGR
                     LDGEEADAIVRTALEEGLELDEIAEIEQATKQPLDLGVIERKNLSKEDRLFVYAVASW
                     MTRLDGVMSPTEQDALARLGDVLKIPERPRALVDGIAQDVANMSDERPARYDLPALRR
                     IIGERMHAAEQLRAAREE"
     gene            552..2054
                     /locus_tag="VFQ35_05630"
     CDS             552..2054
                     /locus_tag="VFQ35_05630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9930141.1"
                     /translation="MTFSNQPTQAAADTPLSVIALQIVNEALQPEATPAALAQMAERD
                     PAFALRIMAMVNSAAFGMTRRVTDVRQASALLGVRGLRSLGLSLALSDMVPSAKGSEV
                     LLSNSLRRATATQLVAKLFGERELDEFFTAGLFLEVGLLAGARADLDRAVQVARSPSP
                     NRLVFERAFGLAEHPMHGAEIARTFHLPESTIEAIRRHHDLDPPSTTVGKAAWLAERV
                     SAIWEGGDLAQSRVAAVEAAQRIGINERSLTEVLKQIPELVHVAAGALDRDAGSQKSY
                     ESLLVDINRGLVELTHSYESVVRQLEILLEEKEQLTKQLAEANDRLEHLAHTDPLTGL
                     ANKRSLEQALVRDLARADRDKTCLSVVVADVDHFKQVNDRYGHAVGDLVLQRVAKILS
                     STLRTGDLATRFGGEEFVLILPGTNAFGAKLAAERIRRAVAQVEFEGPQGKFQVTVSF
                     GVTSVVGPGCSGMSKQVFKEADEALYAAKSGGRNRVVIYAEIAAAGSSNQ"
     gene            complement(2224..>2595)
                     /locus_tag="VFQ35_05635"
     CDS             complement(2224..>2595)
                     /locus_tag="VFQ35_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930142.1"
                     /translation="PPALLVPPTLARPPPPVALLPPRGALELLVPPLEPPPPLLRPPR
                     ELSPTPAEPARAVPPPTAFAPPLLATAPPLALTAAPPLLVLELVPAVAPLGPLPSAPS
                     PQAANVAAPETMAAINLHPRS"
BASE COUNT          463 a          819 c          894 g          419 t
ORIGIN      
        1 ttcgcgccgg gggcacgagc aagctatatt gaaaagccgc gcatggatcc tcaacgcatg
       61 ccgaacgaac ggtcgcgtct tggtcgcgac gtctacctcg cgcttgccgc ggtggggtgg
      121 gccgacggac gactcgacgg cgaagaagcg gacgcaatcg tgcggacggc gctcgaagaa
      181 gggctagaac tcgacgaaat tgccgagatc gagcaggcca ccaagcagcc gctcgacctc
      241 ggggtaatcg agcgcaagaa cctttcgaag gaagaccgct tgttcgtcta cgccgtggcg
      301 tcttggatga cccgcctcga cggcgtcatg agcccaaccg aacaagacgc gctcgcgcgc
      361 ttgggtgacg tcctgaagat cccggagcgc ccgcgcgccc tcgtcgacgg aatcgcccaa
      421 gacgtcgcga atatgtccga cgaacgtccc gcccgttatg atctgccagc gttgaggcgt
      481 atcatcggag agcggatgca tgccgcggag cagcttcgcg ctgcccgcga ggaatgagtg
      541 cgtcgccacc aatgacgttc tcgaaccagc ccacgcaagc cgccgcagat acgccgctgt
      601 cggtcatcgc gctgcagatc gtcaacgagg cgctgcaacc agaggcgacg ccggccgcgc
      661 tcgcacaaat ggccgagcgc gatccggctt tcgcgctgcg catcatggcg atggtcaaca
      721 gcgccgcctt cggcatgacc aggagggtga ccgacgtgcg gcaggcttcg gcgctgctcg
      781 gcgttcgtgg gcttcgcagt ctggggttga gcctggctct gtccgacatg gttccgagcg
      841 cgaagggcag cgaggtgctg ctctcgaact ccctgcgccg agccaccgcg acgcaactcg
      901 tggcgaagct gttcggtgaa cgagaactcg acgagttctt caccgctggg ctgttcctgg
      961 aggtcggtct gctcgccggc gcgcgcgcag atctcgatcg ggccgttcag gttgcccgct
     1021 ccccgtcgcc aaaccggctg gtcttcgagc gcgccttcgg cctcgccgag caccctatgc
     1081 acggcgccga gatcgcgcgg acctttcacc ttccggagtc gaccatcgag gcgatccggc
     1141 gtcaccacga tctggatcct ccgtcgacca cggtcggcaa ggctgcctgg cttgctgagc
     1201 gcgtatcggc catttgggag ggcggcgatc tcgctcagtc gcgtgtcgcg gcggtggagg
     1261 cggcgcagcg cattggcatc aacgagcgat cgctgaccga ggtcctgaaa cagattccgg
     1321 agttggtgca cgttgcggcg ggagcgctcg accgcgacgc agggtctcag aagagttacg
     1381 agagcctgct cgtcgacatc aaccgcggcc tcgtcgagct gacgcacagc tacgaatcgg
     1441 tcgtccgcca gctcgaaatc ttactcgaag agaaggaaca gctcacgaag cagctcgcgg
     1501 aagcgaacga ccgcctggaa cacctcgcgc acaccgatcc cctcaccggg ctcgcgaaca
     1561 agcgctccct cgaacaggct ttggtgcgcg atctggcgcg cgcggatcgc gacaaaacgt
     1621 gcctctccgt cgtcgttgcg gacgtcgacc acttcaagca agtgaacgac cgttacggac
     1681 acgcggtcgg cgacctcgtc ttgcaacgcg tggcaaagat tctttcgtcc acgttgcgca
     1741 cgggagatct ggcgacacgc tttggcggcg aagagttcgt gctgatctta ccgggtacga
     1801 acgcgttcgg cgccaagctc gccgccgagc ggatccgccg agcggtcgcg caggtcgagt
     1861 tcgaaggccc gcaaggcaag ttccaggtca ccgtcagctt cggcgtgacg agcgtcgtcg
     1921 gtccaggctg ctccggtatg tcgaagcaag tgttcaaaga agccgacgaa gcgctctacg
     1981 ccgcgaagag cggcggtcgc aaccgggtcg tcatttacgc cgagattgcc gccgctgggt
     2041 cctcgaatca gtgagcctgg tcgcgtgcgt cggcacgcat ctcctcgcag tgatgggctc
     2101 gggttggggc ctgaaaaatt ccgcggagac ttggccgtgg tgacacgatt tcgggcgcga
     2161 aagcgcgaac ggccaacgtt ttcggcttca cctcgacact gttggggaca ccatgaatca
     2221 gcatcacgaa cgtgggtgta ggttgatggc ggccatagtc tccggcgctg cgacgttggc
     2281 ggcctgcggc gatggcgcag agggaagcgg gccgagcggc gccaccgcgg gcacgagctc
     2341 cagcacgagc agcggaggcg ccgcggtgag cgctagcggc ggcgcggttg caagcaacgg
     2401 cggcgcgaac gccgtcggcg gcggcaccgc gcgtgctggt tccgccggcg tgggcgaaag
     2461 ctctctgggc ggtcgaagca acggcggtgg aggctcgagc ggtggcacga gcagctcgag
     2521 cgcgccgcgt ggcggaagga gtgcgaccgg gggcggcggt cgagcgagcg tcggtgggac
     2581 cagcaatgct ggtgg
//
LOCUS       DASTKS010000273         7966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1828281, whole genome shotgun sequence.
ACCESSION   DASTKS010000273 DASTKS010000000
VERSION     DASTKS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828281"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2750)
                     /locus_tag="VFQ35_05640"
     CDS             complement(<1..2750)
                     /locus_tag="VFQ35_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9930143.1"
                     /translation="MTSLPDWQSRVTSSAPHAHADELPHLVQFYGSDRELTETVTEFL
                     EEGLKAGVPALVIATTEHWAAFRQSLLTRGIDVDGELGAGRIVAVDAQAMLGEILVND
                     SPHWEKFVAKLEPLIEHALASAENSVDHLRAYGELVDILWRDGKHLAALVIEDMWNEL
                     GRKHRFSLYCAYGLGGFYKQAGGIEAVRERHSGVRGRLEESLLAEEMRSLSAEVAQRK
                     AVERDLRQTIAELALQKEALHRAETELRDFVDNAVLPLDVVDPEGRITWANRAQLEML
                     GYSAEEYVGKLAADFHADFGEGAEMSARLRRAHVLNSFPACLRAKDGSLRFVETSSSV
                     YRRDGAVLYSRRFNRDVTTQRSAQRAQAQQETRAERLKRITAVIADAVTTDEVFEALV
                     DRSAEVLEASASALWLIDETRKLARLAKSRGYAEPLAPALACVAIDTSTPVGRVLVDR
                     EPLWFDSPPRRGESAESGETSGAGNVSVALLPLCTQGKVLGALSMTFADRRPLDAAER
                     NLLLLVARSSSQALERWRLLSSERENRRRAELLYGLAAAVMDAEAIEHIFEAALDAIE
                     SALGADRASILLFDPDEVVRFKAWRNLSSEYRAAVEGHCPWPRDARSPQPIVSGDVES
                     DPAMAAYLELFRREGIGAIGFIPLVSGGRLIGKFMVYYTRPRELAASELHLARAIANQ
                     VAAAVVRFNSISELQRSVRFNEIFSGILGHDLRNPLNAIMMAAQTALLRDDPDLHRRS
                     FSKIVASGSRMARMIEQLLDFTRVRLGGGIPLVAREADLLLIVRHAVEELEAAHPTHE
                     IRLSHGHDTRGMWDTDRLAQVFSNLLGNAVQHGETGAGIDVVIDGRDVEFVTITVQNR
                     GSIPAELLKDVFEPLCGGEPRAGSRGLGLGLFITRELVRAHGGDIRVRSEPTEGTTTF
                     AVA"
     gene            3116..4660
                     /locus_tag="VFQ35_05645"
     CDS             3116..4660
                     /locus_tag="VFQ35_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930144.1"
                     /translation="MRDSFEDQEWAAMTDASESETKRLREQLQEHEERLRLALQAAKL
                     GAWEHVPNSGVLHWDARCKQILGFAVDRDVAFEDFMAAVHPDDIAGLLSGINKATDPN
                     GSGECSLQYRVRAGGEERWVEAHGRCAFVAGVPKRINGTLLDITERKRSEDALEEAVR
                     RKDEFLALLGHELRNPLAPIRTAVELMRVRYPELAARELDVIERQLRYVTRLVDDLLD
                     ISRVTRGTLELKLEVCELATLVREAITMVTPLLESRRHRLEVELAHGVFVDADPLRFT
                     QIVANLLNNAAKFTPAGGAISVRSARNGDFFSLTVTDNGSGIEAHLLDRIFEPFVQGT
                     RAATTQGGLGLGLALVKRLVELHGGSVEATSAGLGQGSTFSVRARIADSAPRSVPARV
                     ASEPARVRRILVVDDNEDAAELLGYLLGTLGHTVCVANNGLNALERAAAFAPEVAVVD
                     LGLPLMDGYELAGRLREVCKDEGLRLIALTGYGQPEDRARTRAAGFDAHLVKPVEVAE
                     LEAVLG"
     gene            4748..5077
                     /locus_tag="VFQ35_05650"
     CDS             4748..5077
                     /locus_tag="VFQ35_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930145.1"
                     /translation="MIETLAKDLSTLVFLVEHGANEQALGVLEDGGFAQLEALLAEST
                     WNRGKRRRACGADFAYWLLARLPNRHKARVTTALIAWTIPVRGAPQPSHPFAVRSHLQ
                     VALRAAR"
     gene            complement(5192..6178)
                     /locus_tag="VFQ35_05655"
     CDS             complement(5192..6178)
                     /locus_tag="VFQ35_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9930146.1"
                     /translation="MSRPVEFAKPLVENVFTADPSAHVFEGRIYVYCSHDIDSGKPEK
                     DDGSHFDMVDYRVLSLGGAGSEVVEHSDVLHVRDVPWAERQMWAPDAMFRNGTYYLFF
                     PAKDPQGVFRIGVATSERPGGPFRARPEPIRGSFSIDPCSFLDDDGQAYLYFGGLWGG
                     QLERWQSGSYDANAVGPAPDAPALGPRVARLDANCETIEGNVQEIRILDEHGAPLKAG
                     DTSRRYFEGPWVHRYRDTYYLSYSTGDTHYLVYATSKSPLGPFTYRGRILNPVVGWTT
                     HHSFVEYQGQWFLFYHDSVLSGGVTHLRSVKVVPFQYGADGSIPTIDPATAR"
     gene            complement(6414..>7966)
                     /locus_tag="VFQ35_05660"
     CDS             complement(6414..>7966)
                     /locus_tag="VFQ35_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930147.1"
                     /translation="ERLRLALAAAKMGAFDFDPRSDTLVWDARCRQMCGVEIDVPVER
                     DFALDLVHPDDRNRVLAALARSSDPRGTGELYVEFRVNRASDGELRWLSARGKVHLEN
                     ERAVRLIGTLLDNTELKAIQAARDELLERERAARAEAELANRVKDDFLAIVSHELRTP
                     LNAILGWASIIASKTGDPAFVVKGVEVIHRNARTQARIIEDMLDVSRIIAGKLKLEIR
                     ELDFRSIVEDAIEVVKPSADQKRITLEFAASSLPATLSGDADRLQQVVWNLLSNAIKF
                     TDTGGTVEVRLDDDGALVTLEIRDTGYGISAEFLPRIFERFRQADSSAARKFGGLGLG
                     LTIVRHLVELHGGRVSASSAGLGLGSQFRVELPAHAPVETGETRKSSKFPAAREVLAG
                     LEVLLVEDDGDTREFLKTSLEMEGNVVCAVASADEAIEALKRRSFDVLLSDIGMPGRD
                     GYDLIRSVRARGDGARDVLAIALTAYARDVDRDAALNAGFDAHLPKPLDLARLAATVH
                     GLLDRRSS"
BASE COUNT         1372 a         2591 c         2630 g         1373 t
ORIGIN      
        1 gccacggcga acgtggtcgt tccctcggtc ggctccgagc gcactcggat gtcacctccg
       61 tgcgctcgta cgagctcacg cgtgatgaat aggccgagac cgagtccgcg cgagcccgct
      121 cgaggttctc cgccgcagag cggctcgaag acgtctttga gcagttcagc ggggatagag
      181 ccgcgatttt gcaccgtgat agtaacgaac tcgacgtctc ggccgtcgat caccacatcg
      241 atgccggcgc ccgtttcccc gtgctgcacg gcgttgccga gcaagttgga gaagacctgc
      301 gcgagccgat ccgtgtccca cattccgcgg gtgtcgtgcc cgtgcgacag gcgaatctcg
      361 tgagtggggt gcgccgcttc gagctcttcg acggcgtgtc gaacgatgag caagagatcg
      421 gcctcccggg cgacgagcgg aatgcctccg ccgagacgca cgcgggtaaa atcgaggagc
      481 tgctcgatca ttcgagccat gcgcgagccg ctcgccacga tcttgctgaa cgagcgacga
      541 tggaggtccg ggtcgtcccg cagcagggcg gtctgcgccg ccatcatgat cgcgttcagc
      601 gggttgcgca ggtcgtggcc caaaatgccg gagaagatct cgttgaagcg gaccgagcgc
      661 tgcagttcac tgatcgagtt gaaccgcacc accgccgccg cgacttgatt ggcgatcgcg
      721 cgcgcgaggt gtaactcgga cgcggcaagc tctcggggcc gcgtgtaata gaccatgaac
      781 ttgccgatca gtcggccgcc ggagaccagc ggaatgaagc cgatcgcacc gatgccttcg
      841 cgccggaaca gctcgaggta agccgccatg gccgggtcgc tttccacgtc accgctcacg
      901 atgggctgag gcgagcgagc gtcgcgcggc caaggacagt gcccttcgac cgcggctcga
      961 tattcgctcg agagatttcg ccacgccttg aagcgaacga cttcatctgg atcgaagagc
     1021 aggatcgacg ctcggtccgc gcccagcgcg gattcgatcg cgtcgagcgc agcttcgaag
     1081 atgtgttcga tcgcttcggc gtccatcacc gctgcggcca agccatacag gagctcggcg
     1141 cgacgtcggt tctcgcgctc cgagctcaag agccgccagc gctcgagagc ctgactgctc
     1201 gagcgcgcga cgagcagcag caagttgcgt tcagcggcgt ctagcgggcg tctgtcggcg
     1261 aacgtcatgg acagagcgcc gagcaccttg ccttgagtgc agagcggcag cagcgcaacg
     1321 ctcacgttcc ctgcgccgga ggtttccccg ctctcggcgg actcgccacg ccggggtggt
     1381 gaatcgaacc agagaggctc ccgatcgacc aggacgcgac cgacgggcgt actcgtgtcg
     1441 atcgctacgc aggcgagcgc gggggccagc ggttccgcat agccgcggga tttcgcgagg
     1501 cgcgcgagct ttctcgtctc gtcgatcagc cacagtgccg acgccgaagc ctccaacacc
     1561 tcggcgctgc gatcgaccaa tgcttcgaag acctcgtcgg tggtcaccgc atccgcgatg
     1621 acggccgtga ttcgcttcaa gcgctcggcg cgcgtctcct gctgggcttg tgcgcgttgc
     1681 gccgagcgct gggtggtcac gtcgcggttg aagcggcgcg agtacagcac ggccccatca
     1741 cggcgataca cgctggagct cgtctcgacg aagcggagcg agccgtcctt tgcgcgcagg
     1801 caagccggaa acgagttgag cacgtgcgct cgccggagac gcgccgacat ctcggcgcct
     1861 tcaccgaagt ctgcgtggaa gtcggcggct agcttcccga cgtattcttc ggcgctgtag
     1921 ccgagcatct cgagctgggc gcggttcgcc cacgtgatgc gtccctcggg gtcgaccacg
     1981 tcgagcggga gcacggcgtt gtcgacgaaa tcgcggagct cggtttcggc gcgatggagc
     2041 gcttccttct ggagcgcgag ctcggcgatg gtctgacgta aatcgcgctc gaccgccttg
     2101 cgctgcgcga cctcggcgct gagcgagcgc atctcctcgg ccaggagcga ctcttcgagc
     2161 cgacccctca cgccgctgtg tcgctcacgc accgcctcga tgccgccggc ttgcttgtaa
     2221 aagccgccga gcccgtacgc gcagtagagc gagaagcgat gctttcgccc gagctcgttc
     2281 cacatgtctt cgatgacgag cgccgccaaa tgcttgccgt ctcgccacag gatgtcgacg
     2341 agctcgccgt aagcgcggag gtgatcgacc gagttctccg ccgaagccag cgcgtgctcg
     2401 atgagcggct cgagcttcgc tacgaacttc tcccagtgcg gcgagtcatt gaccaaaatc
     2461 tcgcccagca tggcctgtgc gtcgacggcg acgattcgac cggcgccgag ctccccgtcg
     2521 acgtcgatgc cgcgcgtgag taggctctgc cggaacgcag cccagtgctc ggtcgtcgcg
     2581 atgacgagcg cgggaacgcc ggccttcaga ccttcttcga gaaactccgt gaccgtttct
     2641 gtcagctcgc ggtcgctgcc gtagaactgc accaagtggg gcagctcgtc ggcgtgcgca
     2701 tgtggggcag agctcgtcac gcggctctgc cagtccggca agctagtcat cggcgtcctt
     2761 cctgcgcgac ggcgcagaac agcaaacttg caacgagagt aggccgcgag gcggcgtttg
     2821 caaggtgcgg acgctcggtt ggccgtcatc gtacacaatg gagccccgta cgagtcatgg
     2881 gttggcaagt ccgccacacg cgacacgcac ggcgaacggc gccaaccagc cgtagacgca
     2941 ccctcgagcg ttactgcacc ggcacgtcta cttcgactca ccacgatctt cgaaatctgc
     3001 gtgcgtgttt cgtcagcgcc gcggggcccg aataggcaag ctttacgcgc gcgccgcgcc
     3061 gttaggacgt aactttagag cttcggcggc agcgtgcctt gacctcctgt cgccgatgcg
     3121 ggattccttc gaggaccaag agtgggccgc gatgaccgac gcctccgagt ccgaaacgaa
     3181 gcgcctgcgt gaacagctgc aagagcacga agagcgcctc cgtctcgcgc tccaagcggc
     3241 caagctcgga gcctgggagc acgtgccgaa cagtggagtt ttgcactggg acgcgcgctg
     3301 caagcagatc ctcgggtttg cggtcgatcg agacgttgcg ttcgaagact tcatggccgc
     3361 ggttcatccg gacgacatcg cgggcctttt gtctggcatc aacaaggcga ccgatccgaa
     3421 tggcagcggt gaatgctcgc tccaataccg cgtcagagcc ggcggcgaag agcgctgggt
     3481 cgaagcgcac gggcgctgcg cgttcgtcgc tggcgttcca aagcgcatca acggcactct
     3541 tctcgacata accgagcgca aacgctccga agacgccctg gaagaggcgg tccgtcgaaa
     3601 agacgagttt ctggcgttgc tcggacacga gcttcgtaac cccttggctc cgattcgcac
     3661 cgcggtcgag ttgatgcgcg tacgctatcc cgagcttgcg gctcgagagc tggacgtgat
     3721 cgagcggcaa ctgcgttacg tgacgcgctt ggtcgacgat ttgctcgata tctcccgcgt
     3781 tacccggggc acgctcgagc tcaagctgga agtctgcgag ctcgcgacgt tggttcgcga
     3841 agccatcacg atggtgaccc ctttgctcga gtcgcggcgt caccgcctcg aagtggagct
     3901 cgcgcatggc gtgttcgtcg acgccgaccc tttgcgcttc acgcagatcg tcgcgaacct
     3961 gctgaacaac gcggccaagt tcacgcccgc gggcggcgcg atttcggtcc gcagcgcgcg
     4021 caacggggat ttcttctcgc tcaccgtcac cgataacggc agcgggatcg aggcgcactt
     4081 gctcgaccgc atcttcgaac ccttcgtgca gggcacccgc gccgcgacga cgcagggggg
     4141 cttggggctc gggctcgcgc tcgtcaaacg cctcgtcgaa ttgcatggcg gaagcgtgga
     4201 ggccacgagc gcagggctcg gccagggcag cactttctcc gtgcgcgcac gcatcgccga
     4261 ctcggcaccg cgctcggtgc ctgcgcgcgt cgcatccgaa ccggcgcgcg ttcgacgtat
     4321 tctcgtggtc gacgacaatg aagatgcggc agagctcctc ggatacttgt tgggaacgct
     4381 cggtcacacc gtatgtgtgg cgaacaacgg cctgaacgcg ctcgagcgcg ctgccgcgtt
     4441 cgcgccggag gtcgcggtcg tcgatctggg gctcccgctg atggacggct acgagcttgc
     4501 gggtcggctt cgcgaggtct gcaaagacga gggcttgcgt ctgatcgctc tcaccggtta
     4561 tggccagccg gaagatcgag cgcgtacacg agccgcaggc ttcgacgctc acctggtgaa
     4621 acccgtcgag gtagccgagc tcgaagcggt tctgggctga gcgccagcaa gattcccaga
     4681 aatcgcccat cggcggggaa ggagtgggtt ccgcccacgt aacacctggg accgaaagcg
     4741 agctcgcatg atcgaaacgc ttgcaaagga cctgtctaca ctcgtcttct tggtcgagca
     4801 cggcgcaaac gagcaggcgc tcggcgtgct ggaggacgga ggcttcgctc agctcgaagc
     4861 gttactcgcc gagtcgactt ggaaccgagg caaacggcgc cgcgcgtgcg gtgcggactt
     4921 cgcgtactgg ctgctcgccc gtctcccgaa ccgtcacaag gcgcgggtca cgacggccct
     4981 catcgcgtgg acgatacccg tgcgcggggc gccgcagccg agccacccgt ttgccgtgcg
     5041 ctcgcatctc caggtcgcgt tgcgcgctgc tcggtagagc ctcgcgagcc gtcacgtcgg
     5101 tcgctcgcgt gacatggtcg caggcggcga ctttgcacgg ctcgcgctcg ggtttgggca
     5161 cgagccgttc atggctaagc cgggagtgcg ctcagcgcgc tgtcgcggga tcgatggtcg
     5221 ggatgctgcc gtcggcgccg tactggaagg ggacgacctt caccgagcga aggtgggtca
     5281 cgccgccgga gagcacgctg tcgtggtaaa agagaaacca ctggccctgg tactcgacga
     5341 acgagtgatg ggtggtccag ccgacgacgg ggttcagaat ccgcccgcgg taggtgaacg
     5401 ggccgagggg gctcttgctc gtggcgtaga cgaggtagtg cgtgtcgccc gtcgagtagg
     5461 agagataata ggtgtcgcga tagcgatgta cccagggccc ttcgaagtat cggcgtgacg
     5521 tgtcgccggc cttgagcggc gcgccgtgct cgtcgaggat tcgaatctct tgcacgttgc
     5581 cttcgatggt ttcgcagttc gcgtcgaggc gcgcgacgcg cggcccgagc gccggagcat
     5641 caggcgcggg ccccaccgcg ttcgcgtcgt agctgcccga ctgccagcgc tcgagctgcc
     5701 cgccccacag gccgccgaag tagagataag cctgaccgtc gtcgtccaag aacgaacacg
     5761 ggtcgatgct gaagctgcca cgaatcggct ccgggcgagc gcggaaagga cctccgggac
     5821 gctcgctcgt cgccacgccg attcggaaaa caccctgcgg atccttcgcg gggaagaaca
     5881 ggtagtaggt gccattgcga aacatcgcat cgggtgccca catttgccgc tcggcccagg
     5941 gcacgtcgcg cacgtgcagc acgtcactgt gttcgacgac ttcgctcccc gctccgccca
     6001 acgagagcac gcggtagtcg accatgtcga agtgggagcc gtcgtccttc tccggcttgc
     6061 cactatcgat gtcgtgcgaa cagtaaacgt agatgcgccc ctcgaaaacg tgcgctgacg
     6121 gatcggcggt gaagacgttt tctacgagcg gtttggcgaa ctcgacgggg cgtgacatgg
     6181 cggcgtaatg tagtgcagcc tgcctagctc agcgagccca acccaaccag cgaggcgtac
     6241 gcatgcggcc ggccgattgt atgaggttgg gcgacttttc ggctgaacca agaagctcgc
     6301 ggcgtgtcgg ggagccgagc cgcactgcgc catcgctcct ttgccgaccc cgggtgccgc
     6361 gacccccgcg tgcggctcag cgaggtcagg ggcactcgaa ccaaagcgcg ggtctagctc
     6421 gaacgccggt cgagcaagcc atgtacggtg gcggcgaggc gcgccaaatc gagtggtttc
     6481 ggcaagtggg cgtcgaaacc cgcgttcagg gccgcgtcgc gatcgacgtc gcgcgcgtaa
     6541 gccgtgagcg cgatcgcgag gacgtcacgc gcgccatcgc cgcgcgcccg cacgctgcga
     6601 atcaaatcgt agccgtcacg cccgggcatg ccgatgtcgc tcaggagcac gtcgaaacta
     6661 cggcgcttca aagcctcgat cgcttcgtcc gccgaagcca ccgcgcagac tacgttgccc
     6721 tccatctcga gcgaggtctt gaggaactct cgggtgtccc cgtcgtcttc gacgagcaac
     6781 acctcgagcc cggcgagcac ctcgcgagcg gcagggaact tcgagctctt gcgtgtttca
     6841 ccggtctcga cgggcgcatg cgcgggaagc tcgactcgaa actgcgagcc gagtccgagt
     6901 ccggcgctcg aggcagaaac gcgcccgccg tgcagctcga ccaggtgccg tacgatcgtc
     6961 aacccgaggc ccaagccacc gaacttcctg gccgcggagc tgtcggcctg tcggaagcgc
     7021 tcgaagatcc gaggcagaaa ttcggcactg atgccgtagc cggtgtcgcg aatctcgagg
     7081 gtcacgagcg ccccgtcatc gtcgagccgg acttctaccg tgccgccggt gtcggtgaac
     7141 ttgatggcat tcgacagcaa gttccacacg acttgctgca ggcgatccgc gtcgcccgag
     7201 agggtagccg gcagcgaact cgccgcgaac tcgagcgtga ttcgcttctg gtcggcgctc
     7261 ggcttcacca cttcgatcgc gtcttctacg atgctgcgga agtcgagctc gcgaatttcg
     7321 agcttcaact tccccgcgat gatgcgtgac acatcgagca tgtcctcgat gatccgcgcc
     7381 tgggtacgcg cgttgcggtg aatgacctcg acgcccttga ccacgaacgc aggatcaccg
     7441 gttttggacg cgatgatgct cgcccaaccc aagatcgcgt tcagcggcgt gcgcagctcg
     7501 tggctcacga tggccaggaa gtcgtccttc acgcggttcg cgagctccgc ctcggcccgg
     7561 gccgcccgct cgcgttccaa gagctcgtcg cgcgccgctt ggatcgcctt gagctcggtg
     7621 ttgtcgagca gcgtgccgat caggcgcacc gcgcgctcat tctcgagatg gaccttgccg
     7681 cgtgcgctga gccaccgcag ttcgccatcc gaggctcggt tgacgcggaa ctccacgtac
     7741 agctcgcccg tgccgcgcgg gtcactcgag cgagcgagcg cggcgagcac gcggtttcgg
     7801 tcgtccgggt gcaccaggtc gagcgcgaaa tctcgttcga cgggcacgtc gatttcgact
     7861 ccgcacattt ggcggcagcg cgcgtcccaa accaaggtat ccgagcgcgg atcgaagtcg
     7921 aaagctccca ttttggcggc cgcgagcgcg agccggagcc gctcct
//
LOCUS       DASTKS010000274         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_490044, whole genome shotgun sequence.
ACCESSION   DASTKS010000274 DASTKS010000000
VERSION     DASTKS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490044"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFQ35_05665"
     CDS             complement(<1..233)
                     /locus_tag="VFQ35_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9930148.1"
                     /translation="MTSNSSERLPSAERIASSLRQRIAVAAAGSRLPSVRELMREHGS
                     SPVTIQRAISQLVREGRLQTRPGDGTFVVGVAHS"
     gene            309..1283
                     /locus_tag="VFQ35_05670"
     CDS             309..1283
                     /locus_tag="VFQ35_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9930149.1"
                     /translation="MNAEDNVIVGSRVTVPASERGHRKRGLAWSALGMVGFSLTLTAT
                     RAAVPELGGVFVGFGRAVIAAALALCAIGMLGLSLPKREHVGALLRTAIGIVVGFPAL
                     TAIALRTAPASHAQVFVGLSPLATALVAASRGGERLPASFWRAASLGALSVLLFGWLH
                     AGGSVSSADALLVAAVALVGFGYAEGGRLAREIGGLNVVCWALVLALPVTLPVTLWSM
                     HDLSSLRSASWKAISGFAYVSIVSMFLAFVAWYRGLGMGGVARTSQVQLVMPLLGLGW
                     AALFLGEHLELRTLLASALVSVFAFLGNLSRSSAVAREPATAVSARGA"
     gene            complement(1285..1761)
                     /locus_tag="VFQ35_05675"
     CDS             complement(1285..1761)
                     /locus_tag="VFQ35_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930150.1"
                     /translation="MTRALLPPETEQFLLERIRTHEQLQVAIWLSRHPEESVSVESLA
                     SELSLTVVEAQEAVVALVDAELVARSSEGPARYWYAPAHPTHHVQMRFVGVLHDHYPA
                     DLLRTLALNAMARLRRAAQLACLVTLSADVRRALIRRRDEAPASSRRPSGIAVQLA"
     gene            complement(1986..>2622)
                     /locus_tag="VFQ35_05680"
     CDS             complement(1986..>2622)
                     /locus_tag="VFQ35_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930151.1"
                     /translation="ERDKKKRSLIEWRALGHGYCRLDRAELCLKVYADGASDHPEISR
                     DPGVLADVRKLAERLDVGDRALQFAATSLGTAGADLLYDVYEKGKGSASGAASRAKAL
                     LDEEPAKSHVSAALRALLLLQTAMKKPKCGELKQLMPDISRDVDERALPQLTRLSDRR
                     GCGFLGLSDCYGCLRAGGELTRALESAKANPRPSFAPPRDLLAPATATTAK"
BASE COUNT          387 a          807 c          912 g          516 t
ORIGIN      
        1 gagtgcgcga ccccgacgac gaaggttccg tcgcctggcc gagtttgcag gcgcccttca
       61 cgcacgagct ggctgatggc gcgctgaatc gtgacggggc tcgagccgtg ttcgcgcatc
      121 agctcgcgaa ccgagggtaa gcgcgaacct gcggcggcta cggcgatccg ctgtcggagg
      181 ctgctcgcga tgcgttcggc actcgggagc cgttcgctgc tgttactggt catttgcgac
      241 tataggataa cagttgaaca ttctatgcgc cataattgac gatttaattg gatccgttat
      301 ccttatctat gaatgctgag gataacgtta ttgttggttc acgagtaacg gtacccgcct
      361 cggagcgtgg ccatcgcaag cgtgggctcg catggagcgc tctcggcatg gtgggcttca
      421 gcttgaccct gaccgcgacg cgagcggccg ttcccgaact cgggggagtg ttcgtcggct
      481 ttgggcgcgc cgtgatcgcg gcggcgctcg cgctctgcgc gattgggatg ctcgggcttt
      541 cgctaccgaa gcgcgagcac gttggcgctc tgctgaggac cgcgatcggc atcgtggtgg
      601 gctttccggc tctgaccgcg atcgcgctgc gcacggcgcc tgcctcgcac gcgcaggtct
      661 tcgttgggtt gtctccgctc gcgaccgcgc tcgtagccgc cagccgtggt ggcgagcgct
      721 tgccagcctc gttttggcgc gcggcttcgc tcggcgcgct atcggttcta ctttttggat
      781 ggctgcacgc gggcggatcg gtgagctcgg ctgacgcgct cttggtagcg gctgtggctc
      841 tggtcggctt cggttacgcc gagggggggc gattggctcg cgagatcggc gggttgaacg
      901 tggtgtgctg ggcgctcgtg ctcgcgctgc ccgtgacttt gccggtcacg ctctggtcga
      961 tgcatgacct ttctagcctg cgcagcgcga gctggaaggc catttcaggc ttcgcgtatg
     1021 tatcgatcgt cagcatgttc ctggcgttcg tcgcctggta tcgagggctc ggcatgggag
     1081 gcgtggcgcg aaccagccaa gttcagctcg tgatgccctt gctaggcctc ggctgggccg
     1141 cgctgttcct gggggaacac ctcgaacttc ggaccttgct cgcgagcgcg ctcgtgtccg
     1201 tgttcgcttt tctcggcaac ctgagtcgtt cgtcggcggt ggctcgcgag cccgccacgg
     1261 ccgtgagcgc gcgtggcgcc tgattcacgc cagctgaacg gcgatgccag acgggcgccg
     1321 gctgctcgcg ggcgcttcat cgcgacgccg gatgagggcc cgccgcacgt ccgccgagag
     1381 tgtcaccagg caagcgagct gagccgcgcg gcgcagacgc gccatcgcgt tgagcgccag
     1441 ggtgcgcagg aggtcggcag ggtagtggtc gtgaagcacc ccgacgaaac gcatttgcac
     1501 gtggtgggta ggatgggccg gcgcatacca ataacgggcc ggcccctcgc tgctccgcgc
     1561 gacgagctcg gcgtcgacga gcgcgaccac cgcttcttgc gcttcgacta cggtgagtga
     1621 gagctcgctc gccagagact cgacgctgac tgactcttcg gggtgacgcg aaagccagat
     1681 cgccacctgc aactgctcgt gcgttcgaat tcgctcgagt aggaactgtt cagtctcggg
     1741 agggagcaga gcccgggtca tgtccgtagc ggttcgatga cgatcttgga ccgtggaggg
     1801 cccaaggaaa agccgttttc gacgcgatcg cttaaccttt cgcaactggg gcgtggcgac
     1861 acatccgcgc caaatcgccc ctgtcggtga ggccactttt acagcgcgat gagatggacc
     1921 tgtggcgact tcgcgcgcga gtcattattg cgcgagctgc agtcggcgtc gttcgggtac
     1981 cgccctcact tggcggtcgt ggccgtcgcg ggtgccaaga gatcgcgtgg cggagcgaag
     2041 ctggggcgcg gattggcctt tgccgactcg agcgcccgcg tgagctcgcc gccggcgcgc
     2101 aggcaaccgt aacagtcgga gagccccaag aagccgcagc cgcgccgatc cgagagccga
     2161 gttagctgag gtagcgctcg ctcgtccaca tcgcgagaaa tgtccggcat gagctgcttc
     2221 agctcgccgc acttgggctt cttcatcgcg gtctgcaaga ggagcagggc gcggagcgcc
     2281 gcggagacgt ggctcttggc gggttcttcg tcgagcagcg cctttgcgcg ggacgcggcc
     2341 ccggaagcgc tacccttacc tttttcgtaa acgtcgtaga gcaggtcagc gccggcggtg
     2401 ccgagcgaag tggccgcgaa ttggagcgcg cggtctccca cgtcgagtcg ctcggcgagc
     2461 ttgcgaacgt cggcgagcac gccaggatcg cgagagatct cgggatggtc gctcgcgcca
     2521 tccgcataga ctttgagaca cagctcggcg cgatccagcc ggcaatagcc gtgtccgagc
     2581 gcgcgccact cgatcagcga ccgcttcttt ttgtcgcgct cc
//
LOCUS       DASTKS010000275         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_266248, whole genome shotgun sequence.
ACCESSION   DASTKS010000275 DASTKS010000000
VERSION     DASTKS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266248"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..2083)
                     /locus_tag="VFQ35_05685"
     CDS             complement(188..2083)
                     /locus_tag="VFQ35_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HET9930152.1"
                     /translation="MTQAQMPMSENRKKLPLLDLDAELKRFESEERARLGLPADEHWV
                     EDMANLMFTKAEKGRITLLIGGLTMAHDYLVEGAFKSLGYNVVALDCPDNAALQVGKE
                     FGNRGQCNPTYFTVGNLVKTLIHLRDHKGLTTKQIVDDYVFLTAGACGPCRFGMYITE
                     YRKALRDAGFDGFRVMLFQQSGGLSQATGQESGLELNPKFFIGLLKALVAGDVINGIG
                     YRLRPYEVEPGATDRAIKRAKDICYDALLHQKSILRALLRAKKEFRKVEIDLTQVRPK
                     VAIIGEFWAMTTEGDGNYQLQRFLEKEGAEADIQFVSAWILYNIWEASFDTKTRETLR
                     GLDGGRTGLKGTGEYDIFKREVGLYLADKALRAAFQVFAYAGGFYGYHLPDMHEVAEV
                     AAPYYNNDLRGGEGHMEVGKLILNVVKSKATMTLSVKPFGCMPSSSVSDGVQTLITEK
                     LPGSIYCAVETSGDGAVNFQSRVQMYLFKARIAAQAELEKALEDTGLSIEGVRDFLSK
                     HPRFKDSLHRPAHRVTTTTADLVYEIADVIGKSKLDLAVERTKRTGTFVVNALRGGLA
                     RAPETSQKAFAFAKRAGSELFGIAKEKAPGVADQALGAARERLGRVLPFARREKVSEY
                     PVAVAAE"
     gene            complement(2101..>4145)
                     /locus_tag="VFQ35_05690"
     CDS             complement(2101..>4145)
                     /locus_tag="VFQ35_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HET9930153.1"
                     /translation="LGRKFVLQGGTQYNLAAVKAQVDYIKERVPGAEVFVHPHTGEAG
                     AIGAAIETLRRYKRTGKTRFIGIDATLDLSYTATTDESTTCHFCPNECKRTFIDTQRP
                     DGSTTRYISGFSCEKGTVESKEAMLSLVAERKKIAAAYPNLVQYEANQAFVHFYKPEP
                     QPAPGTPVRDVRVTKSLFRVKREEYTRAFQRSSEEAAKRRKSIRIGMPRVLNMYSTAP
                     FFRTYFEALGIGRSNIVFSEETTEEMWVEGGKYGSIDPCFPSKVVQAHIHNLLFHKHE
                     PERNRALNYLFFPILTHVANFVADTMDNASCPIVAGTPDVIKAAFTKEVDFFKQRNME
                     YLDPALSFVEPTLLARRMFETWGARLGITEDESDHACREGWKALAEFDRDLQEKGRAI
                     LETVESENRIAILVLNRPYHSDPGMNHGIPEEFQVLGYPILSIRSIPKTREYLDRYYK
                     DELEKGVIKSPLELNHLWPENYSANSAQKVWGANFAAHHPNVAVLDLSSFKCGHDAPT
                     YGLVDGILQTSRTPAAALHDLDANKPGGSIKIRVKTYAHSLRLQEERLEDLTVKRREL
                     AHNIDKKRLELLELKQAQLTSLSQRDPEIERQIAELGERVKSYSATKVTVAELPAGVV
                     QLGRRREDGSIERLGAHKPHPHAHHHHHHHHGTHHHPTPAQPSQDTEAHAALAASV"
BASE COUNT          636 a         1271 c         1388 g          850 t
ORIGIN      
        1 tcgacgtgct cacgttttcg tacacgcaga agcgggtggg cgacagcgtg ctcgagctgc
       61 gggcgagctg gctggggctt tcgacgcagc caaccatcca gtagctcgct cgtcgcgaga
      121 gctcgaggag cgcagctcgc ggcgcgaacg accccgagca cgaggccgtt cgcgtcgctc
      181 gctcgtctca ctccgccgcg accgctactg ggtactcgct gaccttttcg cgccgagcga
      241 agggcagcac gcgaccgaga cgctcccgag ccgcaccgag cgcttggtcg gcgacgccgg
      301 gcgccttctc cttggcaatc ccgaaaagct cgctgccggc gcgcttggcg aaggcgaacg
      361 ctttttggct agtctccggg gcacgcgcga gcccaccgcg gagcgcattg acgacgaacg
      421 ttccagtgcg cttcgtccgc tcgacggcga ggtcgagctt gctcttgccg atgacgtcgg
      481 cgatttcgta aacgagatcg gcggtcgtcg tggttacgcg atgcgcgggg cgatgcagcg
      541 agtccttgaa gcgcggatgc ttgctcaaaa agtcgcgtac gccttcgatc gagagcccgg
      601 tatcttcgag cgccttctcg agctcggcct gcgccgcgat gcgcgccttg aagagataca
      661 tttgcacgcg gctctggaag ttcacggcgc catccccgct cgtctccacg gcgcagtaga
      721 tggatcccgg caacttctcg gtgatgagcg tttggacgcc gtcgctcacg ctcgagctcg
      781 gcatgcagcc gaagggcttc acgctgagcg tcatcgtggc cttgctcttc acgacgttga
      841 ggatgagctt cccgacctcc atgtggcctt cgccaccgcg gagatcgttg ttgtagtacg
      901 gggccgccac ttccgcgacc tcgtgcatgt cgggcaggtg gtagccgtag aagccgcctg
      961 cgtacgcgaa cacctggaag gcagcgcgca aggctttgtc ggccaagtag agcccgacct
     1021 cgcgcttgaa gatgtcgtac tcgccagtgc ccttcaaccc agtgcgccca ccgtcgagtc
     1081 cgcgcagcgt ctcgcgtgtc ttggtgtcga agctcgcttc ccagatgttg tacaggatcc
     1141 acgccgacac gaactgaatg tccgcttccg cgccctcctt ttcgaggaag cgctgcagct
     1201 ggtagttgcc gtcgccctcc gtagtcatcg cccagaactc gccgatgatg gcgaccttgg
     1261 ggcggacctg ggtcagatcg atttcgactt tgcggaactc tttcttggcg cggagcagcg
     1321 cgcgcaaaat cgacttctgg tgaaggagcg cgtcgtaaca gatgtccttg gcgcgcttga
     1381 tggcgcgatc ggtcgcgcca ggctccacct cgtacgggcg aagccgatag ccgatgccgt
     1441 tgatgacgtc gccggccacg agcgccttca agagaccgat gaagaacttc gggttcagct
     1501 cgagcccaga ctcttgcccc gtcgcttggc tgaggccgcc cgactgctga aatagcatca
     1561 cgcgaaagcc gtcgaagccg gcgtcgcgca gcgccttgcg atactcggtg atgtacatcc
     1621 cgaagcggca ggggccgcac gcgcctgcgg tgaggaacac gtagtcgtcg acgatttgct
     1681 tcgtcgtgag ccccttgtga tcccgcaagt ggatgagcgt cttcacgagg ttgccgaccg
     1741 tgaagtacgt cgggttacac tgcccgcgat tgccgaactc tttgccgacc tgcagcgcgg
     1801 cgttgtcggg gcagtcgagc gcgaccacgt tgtacccgag gctcttgaaa gcgccttcga
     1861 cgaggtagtc gtgggccatc gtgaggccgc cgatgagcaa cgtgatgcgg cccttctcgg
     1921 ctttggtgaa catcaagttc gccatgtcct cgacccaatg ctcgtctgcg ggtaggccga
     1981 ggcgcgcgcg ctcctcggac tcgaagcgct tgagctcggc gtcgaggtcg agcagcggaa
     2041 gcttcttccg gttttcactc atgggcattt gtgcttgcgt catggcttcc tcttgttcgg
     2101 ttagacggat gcagcgagcg ccgcgtgcgc ttcggtgtcc tggctcggct gggccggagt
     2161 cgggtgatgg tgggtgccgt gatggtgatg atgatggtgg tgagcgtgag ggtgaggttt
     2221 gtgcgcgccg aggcgctcga tgctgccgtc ttcgcggcgg cgcccgagct gaacgacgcc
     2281 ggcgggcagc tcggcgacgg tgaccttcgt cgcgctgtag ctcttcacgc gctccccgag
     2341 ctcggcgatt tgtcgctcga tctccgggtc gcgctgcgaa aggctcgtga gctgtgcttg
     2401 cttgagctcg agcagctcga gccgtttctt gtcgatgttg tgcgcgagct cgcgacgctt
     2461 gacggtgagg tcctcgagcc gctcttcttg cagccggagc gaatgcgcgt acgtcttgac
     2521 gcggatcttg atcgagccgc ccggcttgtt cgcgtcgagg tcgtgcaagg ccgcggccgg
     2581 ggtgcgcgat gtctgcaaga tgccgtcgac gagcccgtag gtaggcgcgt cgtgcccgca
     2641 cttgaagctc gatagatcga gcacggcgac gttcgggtga tgcgccgcga agttagcgcc
     2701 ccaaaccttt tgcgcgctgt tcgccgagta gttttccggc cagaggtggt tcagctcgag
     2761 ggggctcttg atgacgccct tctcgagctc gtccttgtag tagcggtcga ggtactcgcg
     2821 cgtcttcggg atggagcgga tgctcaggat cgggtagccg aggacctgga actcctctgg
     2881 gatgccgtgg ttcatgcccg ggtcggagtg ataggggcgg ttcaggacca ggatcgcgat
     2941 gcgattctcc gactcgaccg tctcgaggat cgcgcggccc ttctcctgca ggtcgcggtc
     3001 gaactccgca agcgccttcc agccttcacg gcaggcgtgg tcgctctcgt cttcggtgat
     3061 gcccaggcgc gcgccccacg tctcgaacat acgccgcgcg agcagagtcg gctcgacgaa
     3121 gctgagcgcg gggtcgaggt actccatgtt gcgctgcttg aagaagtcga cctccttcgt
     3181 gaacgcggcc ttgatgacgt cgggtgttcc cgcgacgatc gggcacgagg cgttgtccat
     3241 cgtgtcggcg acgaagttcg cgacgtgggt gaggatcgga aaaaagaggt agttgagggc
     3301 gcggttccgc tccggctcgt gcttgtggaa gagcagattg tggatgtgcg cctgaacgac
     3361 cttgctcggg aagcacggat cgatggaccc gtacttgcca ccctcgaccc acatctcttc
     3421 ggtcgtctct tcgctgaaga cgatgttcga gcgaccgatg ccgagcgcct cgaagtaggt
     3481 gcggaagaac ggcgccgtgc tgtacatgtt gagcacgcgc ggcatcccga tgcggattga
     3541 cttccggcgc ttcgctgcct cttcgctgct gcgctggaag gcgcgcgtgt actcctcgcg
     3601 cttcactcgg aacagcgact tggttacgcg cacgtcgcgg acgggtgtgc ccggcgcggg
     3661 ctgcggctcc ggtttgtaga aatgcacgaa cgcttggttg gcctcgtatt ggacgaggtt
     3721 cggatacgcg gccgcgatct tcttgcgctc ggcgacgagc gacagcatcg cttctttgct
     3781 ctcgaccgtg cccttctcgc aggagaagcc ggagatgtag cgagtggtcg agccgtcagg
     3841 tcgctgcgtg tcgatgaacg tgcgcttgca ctcgttcgga cagaagtggc acgtcgtcga
     3901 ttcgtcggtg gtcgccgtgt agctcaggtc gagcgtcgca tcgatgccga tgaagcgcgt
     3961 cttgccggtg cgcttgtagc ggcgcagcgt ctcgatggcg gcgccgatcg ccccggcttc
     4021 acccgtgtgc gggtgcacga acacttctgc gcctggcacg cgctccttga tgtagtcgac
     4081 ctgggccttc accgcggcga ggttgtactg agtcccgccc tgcaacacga acttgcgacc
     4141 cagcg
//
LOCUS       DASTKS010000276         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_713007, whole genome shotgun sequence.
ACCESSION   DASTKS010000276 DASTKS010000000
VERSION     DASTKS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713007"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFQ35_05695"
     CDS             complement(<1..276)
                     /locus_tag="VFQ35_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930154.1"
                     /translation="MSYERLEAHHRKLSQLRHTEAIVAWDEAALMPPGGGEARADALA
                     TLRGLIHECATDARLGDWFAAAEAQSGALGTWQSANLREMRREWTRSN"
     gene            complement(598..1593)
                     /locus_tag="VFQ35_05700"
     CDS             complement(598..1593)
                     /locus_tag="VFQ35_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9930155.1"
                     /translation="MVYVPSPQRYERPSLYRRCGKSGLKLPAVSLGLWHNFGDSDVFE
                     NARSMLRRAFDLGVTHFDLANNYGRPAGSAERTLGRILKLDFGAHRDELIISTKAGYD
                     MWPGPYGDFGSRKYLLASLDQSLARLGLPYVDIFYHHRPDPETPLEETCGALAQAVRS
                     GRALYAGLSNYGPEATARAAELLRQMGTPCLIHQPRYSMFDRRPEQGLLQVLEREGIG
                     AIVFSPLEQGLLSERYLHGMPEDARAVRDGRFLRPEHITEPKLEKVRKLKALAAERGQ
                     SVAQLALAWTLRNPVVTSALVGASSAKQIEENVGVIDKLELSAEELARIESVLRD"
     gene            2447..>3181
                     /locus_tag="VFQ35_05705"
     CDS             2447..>3181
                     /locus_tag="VFQ35_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9930156.1"
                     /translation="MLPGVMLKPGEFIRDLSKAEPGRTVTARGWVKTRRDSKGVHFVQ
                     LGDGSCFKDLQVVFPSELFSAASLEHVTTGACIAVTGELVASPAAGQRVELSAKKFEV
                     LGGADSTYPLQKKGHSFEFLREIGHLRPRSNTFGAVFRVRNALSFAIHRFFQERGFVY
                     VHTPIITASDCEGAGAMFGVSTLDPEKLPRTEQGRVDYAQDFFGKRAYLTVSGQLEGE
                     AYALAFSNIYTFGPTFRAENSNTPRHL"
BASE COUNT          527 a         1057 c         1054 g          543 t
ORIGIN      
        1 gttcgagcgc gtccactcgc ggcgcatctc gcgcaaattc gcggactgcc acgtgccgag
       61 cgcgccgctt tgagcctccg ccgccgcaaa ccaatctccg agccgcgcgt ccgtcgcgca
      121 ctcgtggatc aacccgcgca acgtcgcgag cgcatccgct cgtgcctcgc ctccaccggg
      181 cggcatcaac gccgcttcgt cccacgccac gatcgcctcc gtgtgacgca gctgagagag
      241 ctttcggtgg tgcgcctcga gcctttcgta cgacatgcgg gagagtttag acagcgcacg
      301 ccgtagacac caatttggct tgaattgcgg cttgcttggg tcgccccggc gcggctcact
      361 tgcgaagttt cgcgcggcaa aaggtgcctt tggggcggcc cgtcccgcgc tttcggtcac
      421 cgttcaggcc gcggcgccgc tcgaacttct ccaagtcgac cgcgagccgc gtggcgaacc
      481 gggcattcga agttcgcacc gctaacgcgg gcgcggcagc aaactttgac gcgcgcagcg
      541 ccgcccggaa cgtgcgccgc gcaaatctcg agcgcttcgt ttcgagtcgc ggtcggctca
      601 gtcgcggagg acgctctcga tgcgcgcgag ctcttcggcg ctgagctcga gcttgtcgat
      661 cacgccgacg ttctcttcga tttgcttcgc gctgctcgcg ccgaccaacg ccgacgtaac
      721 cacggggttt cgcagtgtcc acgcgagggc aagctgcgcc accgattgtc cacgctcggc
      781 tgccagcgcc ttcaacttac gcaccttctc gagcttcggc tcggtgatgt gctccgggcg
      841 caaaaagcgc ccgtcgcgca ccgcgcgcgc gtcctccggc ataccgtgca ggtagcgctc
      901 ggagagcagg ccctgctcga gcggtgagaa cacgatcgcg ccgatgcctt cgcgctccag
      961 cacttgcaag agaccctgct ccggccgtcg atcgaacatg ctgtagcggg gctggtgaat
     1021 gaggcacggc gtgcccattt gccgcaagag ctccgcggcg cgcgcggtcg cctcggggcc
     1081 gtagttcgag aggccggcgt acagcgcgcg gccagaacga acggcttgcg cgagcgctcc
     1141 gcacgtctct tcgagcggtg tctccgggtc ggggcgatgg tgataaaaga tgtcgacgta
     1201 aggcagcccg agccgcgcca agctctgatc gaggctcgcg agaagatatt tgcgggagcc
     1261 gaaatcgccg tacgggcccg gccacatgtc gtagcccgcc ttcgtcgaga tgatgagctc
     1321 gtcccggtgt gcgccgaagt cgagcttcaa gatgcggccg agcgtgcgct cggcggaccc
     1381 tgccgggcgc ccgtagttgt tcgcgaggtc gaagtgagtg acgccgaggt cgaacgcacg
     1441 gcggagcatg ctccgcgcgt tttcgaagac gtcgctgtca ccgaagttgt gccacaagcc
     1501 gagcgatacc gccggcagct tgagaccact cttaccgcag cgccgataca gcgagggccg
     1561 ctcgtagcgt tggggtgacg gaacgtagac cacggcttcg aacctccgga gcgcgcgctg
     1621 cgagacgcga gcgcgaacga gcgccgagca tggcggaatc gctcgacctc cgcaagcctg
     1681 cccgccgaaa acacgctccg ttctgcgcac cgcgcgcgcc gtgcgctcgc gcctggaacc
     1741 ggtaccgcat tttggccgag ggcgacggcg tggcacggtt cgaggtcgaa gggccgcacc
     1801 gcgaaccaaa atcgggtgat tccctcagca cttggtcacc aaggcaattt ggtgcctggc
     1861 ggacgcggga ttctcggaga ctctggctta ccgcgcgggt cgtcgactgg tcgcggcgca
     1921 cccgggggtt ggcgcacgtc gggttcggcg tagcactggg tgccgtagcg aggtcctgac
     1981 tggcagtaag tgcccccgcc ggggccgcac cccgcgcagg tgaaaacgat cgccgcgaag
     2041 acgagcgcga gcgctgtggt cgccgagcgt cgtggcgtcg gaaagggggt gagttgcaac
     2101 atgggaacct cgcagggtcg ggaagctcgt gagcgccgta cgcggctcga ctcggagaga
     2161 gcaagcgctg ggccaaggtg aaatagcgct ggttttgccg aggtcccctg cgggtgtgaa
     2221 cgacggttcg agtcgctcga acgacacctg cgaggctgaa gccaccgatg gccaacaccg
     2281 caccttctgc gcgcaaggtg cgcggcctac cgacgctgct cggtgttagc ggttccaagt
     2341 gtcccgaatt ccccggccct gttcggttcc gagcactgcg atcgcctggc gcgtttcgct
     2401 ccatccgccc tgaaaattcg gcaccttggg gcgaaacgct cggggtatgc tgcccggcgt
     2461 catgttgaag ccgggtgagt tcattcgcga tttgagcaag gccgaaccag gtcgcaccgt
     2521 caccgcccgc ggatgggtga agacgcgtcg cgatagcaaa ggcgtgcact tcgttcagct
     2581 cggcgacggg agctgcttca aagacctgca ggtggtcttc ccgagcgagt tgttttccgc
     2641 cgcttcgctc gagcacgtca cgacgggcgc ttgcatcgcg gtgaccggcg agctcgtggc
     2701 gtcgcccgcc gccggacagc gcgtcgagct cagcgccaag aagttcgagg tgctcggcgg
     2761 tgccgactcg acgtaccccc tacaaaagaa gggccactcc ttcgagttct tgcgagaaat
     2821 tgggcacctg cgcccccgct ccaacacgtt tggcgcagtc ttccgggtcc gcaacgcact
     2881 gtcgttcgcg atccatcgct tctttcaaga gcgcggcttc gtctacgtgc acacgcccat
     2941 catcacggct tcggactgcg aaggcgcggg cgcaatgttc ggagtcagca cgttggaccc
     3001 ggagaagctc cctcggaccg agcagggtcg cgtcgactac gcgcaagact tcttcggcaa
     3061 gcgcgcgtac ctcaccgtga gcggccagct cgagggagag gcgtatgcgc tcgcgttcag
     3121 caacatctac acgttcgggc cgacgttccg cgccgagaac tccaacacgc cgcggcacct
     3181 g
//
LOCUS       DASTKS010000277         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_266695, whole genome shotgun sequence.
ACCESSION   DASTKS010000277 DASTKS010000000
VERSION     DASTKS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266695"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFQ35_05710"
     CDS             complement(<1..530)
                     /locus_tag="VFQ35_05710"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9930157.1"
                     /translation="MTLTDSTLTLPNERGSRPPLGVLLRVLNAPAKPALFQLRAGACR
                     VGAASHMDLVIDDETVSRQHLRIELVPEGVSVIDEGSRNGTFYREQRLLSAVLAPGSR
                     LLLGRVELAIELDARTLCEFEPSGAIGYGGLIGNSEPMRGMFAILKRLEGSLLSVLVS
                     GESGTGKELVARSIHD"
     gene            662..1993
                     /locus_tag="VFQ35_05715"
     CDS             662..1993
                     /locus_tag="VFQ35_05715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930158.1"
                     /translation="MERGGKVLGGRYRLERSLGAGSMGCVWAATHLELDAPVAIKVAH
                     PGPERIRRTAASRREARAAARLRSPHVVQVFDFGTDDGVAFIAMELLVGESLRARLDS
                     EVRLSPRQAGEIVQQAAAALELAHRERIVHRDIKPSNLFLVREKAREVVKVLDFGVAK
                     WMDVESSTRSRTDNAGLVGSAGYMSPEQARGQGVDHRSDVWALGVVAYEMLLGARPFQ
                     GVSIPETLAIICTGRYVSPSALVGSAYAVLDPVFARVLDPDREGRYQSVGEFASAFTD
                     AVLSLPSPDTRLEPTARESPEDSFGRDAETASLRVVATSAPAEKRTHSRKVTAILLGA
                     SLAGAALYDRWSVKAAPLSPVGLTARRSPAVISSVSAALPVHQAAEPAASAAVPASAE
                     APPVATSRRRVPVAPIRRPRLAASAQVEATVPSAPAPSRIDPVFGLEVPER"
     gene            1990..>2508
                     /locus_tag="VFQ35_05720"
     CDS             1990..>2508
                     /locus_tag="VFQ35_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930159.1"
                     /translation="MRRTARARFLGSLSVVVGTLCLPAVARSEPASDEPRALLSQNAA
                     AVEAGRAALDAYQRRDWAEAFEQFHAAEALAHSPVFLLFMARTKERQGELLAALDLYA
                     RVLAEPTVSEMPDAWLRAIEAAGVERLAVLARVPSLIIVLARNHPPGTIVMVDDVPFE
                     RAETELLLNPGAH"
BASE COUNT          393 a          829 c          860 g          426 t
ORIGIN      
        1 tggtcgtgga tggagcgggc gacgagctct ttgccggtcc ccgattcacc cgagaccagc
       61 acgctgagca gggagccttc gagtcgtttg aggatcgcga acatcccgcg cattggctcg
      121 gagtttccga tcaggccgcc atagccgatc gcccccgatg gctcgaactc gcacaaagtg
      181 cgcgcgtcga gctcgatcgc gagttcgaca cgaccgagta ggagccggct ccccggcgcg
      241 agcacagcgc tcaacaggcg ctgttcgcga taaaacgtgc cgttgcggct accttcgtcg
      301 atgacgctga ccccctcggg gacgagctcg attcgaaggt gttgtcgcga caccgtttcg
      361 tcatcgatga ccaggtccat gtgactcgcc gcgccgacgc gacacgcgcc cgcgcggagc
      421 tgaaacagcg ctggcttcgc cggcgcgttc aggacgcgca gcagtacccc caggggtggc
      481 ctcgacccgc gttcgttcgg cagggtgagg gtcgaatcgg tcagggtcat cgccgcgcga
      541 tttatacccg accttcggga gcgacccaga ggccgcccct gacacggcca accaagttcg
      601 gaactttgcc gccaatttgt tcgatcttgc agccgtcggc cgcttggaac atggtcgggg
      661 agtggagcgt ggaggaaagg tcttaggcgg acggtaccgc ctggagcggt ctctgggcgc
      721 aggctccatg ggctgcgtct gggccgcgac gcacctggag ctcgacgcgc cggtcgcgat
      781 caaggtggcc catccggggc ccgaacgaat ccgccgaacg gccgctagcc gtcgcgaagc
      841 gcgggccgcg gcgcgcctcc gcagccccca cgtcgtccag gtcttcgact tcgggacgga
      901 cgacggggtc gccttcattg cgatggagct cctcgtcggt gagtcgctga gagcgcgcct
      961 ggactccgag gtgcggcttt ctccacggca agccggtgag atcgtgcagc aagcggcggc
     1021 cgctctcgag ctcgcgcatc gcgagcgcat cgtgcaccgc gatatcaagc cgagtaacct
     1081 cttcttggtc cgcgagaaag cgcgcgaagt agtcaaggtg ctcgattttg gcgtcgcgaa
     1141 gtggatggac gtcgagtcca gcacccggtc gcgaaccgac aacgccggtt tggtgggctc
     1201 cgcggggtac atgagcccgg agcaggcccg cggccaaggg gtcgatcatc gctcggacgt
     1261 gtgggctctc ggggtggttg cctacgagat gctgctcgga gcgcgtccgt tccagggcgt
     1321 gagcatcccc gagacgctgg ccatcatttg caccggtcgg tacgtcagcc cttcggcact
     1381 tgtcggtagt gcttacgccg tgctcgaccc cgtgtttgcg cgcgtcctcg atccagatcg
     1441 agaggggcgc tatcaatcgg tgggcgagtt cgcgagcgcc ttcacggatg cggttctgag
     1501 tttgccgtcc ccggacacgc ggctcgagcc cacggcccga gagagcccgg aggacagctt
     1561 cggtcgcgac gccgagacgg caagcctgcg agtggtcgcg acgagcgctc ctgccgaaaa
     1621 gcgaacgcac agccgcaaag taaccgccat cttgctggga gcgagcttgg cgggagccgc
     1681 gctctacgat cgttggtcgg tgaaagcggc gccgctgtcg cccgttggcc taacagcccg
     1741 caggtcgcca gccgtcataa gctcggtcag cgcggctctg cctgtgcatc aagcggccga
     1801 gcccgccgcg tctgccgctg tgcccgcatc ggccgaagca ccgcccgtgg ctacgtccag
     1861 gcggcgagtg ccggtcgcgc caatacgccg accgcgcctt gccgcgagcg cccaggtaga
     1921 agcgacggtc ccctcggcac ctgcaccgtc tcggatcgat ccggtgttcg gcctagaggt
     1981 tcctgagcga tgaggcgaac ggcgcgcgcg cggtttttgg gatcgctgag cgtcgtcgtg
     2041 gggacgctct gccttccagc ggtggctcgc agcgaacccg catcggacga gcctcgcgct
     2101 ctgctgtccc agaacgccgc cgcggtcgaa gcaggtcgag cggcgctcga cgcctatcag
     2161 cgcagggatt gggcagaggc gtttgagcag tttcatgcgg cggaggcgct cgcccactcg
     2221 ccggtcttcc tgttgttcat ggcgcgcacc aaagagcgtc agggcgagct actcgcggcg
     2281 ctcgacctct acgctcgcgt gctcgccgag ccgaccgtga gcgagatgcc cgatgcgtgg
     2341 cttcgcgcca tcgaagcggc gggcgtggag cgcctggccg tgctcgcccg cgtcccaagt
     2401 ctgatcatcg tgctcgcgcg aaatcacccg ccgggtacca tcgtgatggt cgacgacgtg
     2461 ccgttcgagc gcgctgaaac ggagctgctc ttgaatccgg gcgctcac
//
LOCUS       DASTKS010000278         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_713302, whole genome shotgun sequence.
ACCESSION   DASTKS010000278 DASTKS010000000
VERSION     DASTKS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713302"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFQ35_05725"
     CDS             <1..1254
                     /locus_tag="VFQ35_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HET9930160.1"
                     /translation="SFAGRARYLLASEEVEPGHGWDHRAVSILKTNPTALALGEAVVR
                     TYQAQAVANQDSARITLALTDLDKVPELSRALATLSGAMQAPIDDHAPLVGRARAGIT
                     TFGQIPGGPSTGMVDLLEFVDTLAGLDPTLQAPIAAVRGALGSAVVNKVSGPALAGVG
                     GLSVFFPSVQKGYDPDYASLGVANTWRSFLSAYYGAASSLTQSPVFTNPNKEALVTSV
                     LGALHVAGQLQSGTFDNLASTTFDFGLVGQDDTIYVLGDEPADVDRNGLVTKDWNESA
                     LQIVQGTTSDFAYYVLEATDNGLVSLSVPLGYEDESSVEFVLLQLVFQLDGTLVSSSY
                     YVQTDGAWAELVPAAGGTYRTLIPTLVSGAAEVSWEPQVEVFDSSNPLDLRFADLPAG
                     TNVFVELTASDFSEQSDSVISAVRL"
     gene            1258..1959
                     /locus_tag="VFQ35_05730"
     CDS             1258..1959
                     /locus_tag="VFQ35_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930161.1"
                     /translation="MTRLTRASVALAALLAACSRPSASSGEAANGSAAVAAPKPAATP
                     PASVSGAPGPSAVSSETLPSTSPALAASVGEEGAAPKGTPSPATETEANTEVSGCLAS
                     ASGTPTRAASSAGTPGVELVQGAPPKLVHRLEHACCLHSQTRILRRDRAIQVTEQLSG
                     TPCRCKCSSTLSTELRVPAGSYEISLVLEQGGTRSEVYRGALDVAASSGGSVASPTTA
                     GSQLPRTIHVPAAKH"
     gene            complement(1991..2374)
                     /locus_tag="VFQ35_05735"
     CDS             complement(1991..2374)
                     /locus_tag="VFQ35_05735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9930162.1"
                     /translation="MVDMNDKRVAPREPVHFVAEIELDGERLGCGVSRNASGNGLLLL
                     TAAEPPVGSKLLLRLYVPREAEPRVLDATVVRTEKISPTEGLVWSYRLGIHLDAPPAD
                     MQELIESIVKRPSAGPGSRSGSSTA"
     gene            complement(2529..2828)
                     /locus_tag="VFQ35_05740"
     CDS             complement(2529..2828)
                     /locus_tag="VFQ35_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9930163.1"
                     /translation="MSNASKRSEGAAEETGGKIKEVVGKVVGSERMEAEGRARKLEGQ
                     AKQESAKAAERATGKAQQAVGAIKNRVGALIGDDELQAKGKVKELEGEARERANR"
BASE COUNT          570 a         1126 c         1043 g          634 t
ORIGIN      
        1 tccttcgctg gtcgcgcccg ctacctgctc gcatccgaag aggtcgagcc tggccatggc
       61 tgggaccatc gcgcggtttc gattttgaag acgaatccga ccgcgctcgc gctcggcgaa
      121 gcggtggttc gcacgtatca agcacaagcc gtcgcgaatc aggacagcgc tcgcatcaca
      181 ctcgcgctca ccgacctcga caaggttcca gagttgtctc gagcgctcgc gacgctatcg
      241 ggagccatgc aagcgcccat cgacgatcac gcgcctctcg ttggtcgcgc ccgcgccggc
      301 atcaccactt tcggccaaat ccccggaggg ccgtcgacgg gcatggtcga cctcctcgag
      361 ttcgtcgaca cgctcgcggg gctcgatccg acgctgcaag cgccaatcgc ggccgtgcga
      421 ggcgcgctcg gctccgcggt ggtgaacaag gtttcgggcc ctgccctcgc aggcgtaggc
      481 ggcttgtctg tgttcttccc ctccgtgcag aaaggctacg atccggacta cgcatcgctc
      541 ggcgtggcga acacctggcg cagcttcttg tcggcgtact acggcgcggc gagtagcctc
      601 actcagagtc ccgtctttac caatcccaac aaggaggcgc tcgttacttc ggtactgggc
      661 gccctgcatg tggccgggca gctgcaaagc gggaccttcg acaatctcgc gtcgacgacc
      721 ttcgacttcg ggctcgtggg tcaagacgac acgatttacg tgctcgggga cgagccggcc
      781 gacgtagatc gcaacggctt ggtgaccaag gattggaacg agagcgctct gcagatcgtc
      841 cagggtacca ccagcgactt cgcctattac gtgctcgagg cgacggacaa cgggctcgtg
      901 tcgctctcgg tgccgctcgg gtacgaggac gaaagctcgg tggagttcgt gttgcttcag
      961 ctcgtattcc aattggacgg cacgctggtc tcgagcagct actacgttca aaccgacggt
     1021 gcttgggcgg agctcgttcc ggccgcgggg ggaacctatc gaaccttgat cccgacactc
     1081 gtgagcgggg ctgctgaggt gagctgggag ccgcaagtcg aggtcttcga ttcgagcaat
     1141 ccgctcgatc tgcggttcgc cgatttgcct gcaggtacca acgtcttcgt cgagctcacc
     1201 gcgagcgact tctccgagca gagcgactct gtcatcagcg cagtgaggct ttgaacgatg
     1261 acgcgactca ctcgagcttc cgttgcgttg gcggctctgc tcgcggcgtg cagcaggcca
     1321 tcggcgagca gcggagaagc ggcgaacggc tccgccgcgg tcgcggctcc gaagcccgcg
     1381 gcaacgcccc cggcgagtgt atcgggggct ccagggccgt ccgcagtatc gagcgagacg
     1441 ttgccgagca cgtcgccggc gttggctgcg tcagtcggcg aggagggtgc agcgcccaaa
     1501 ggcaccccat cgccggctac cgagacggaa gccaatacgg aggtgagcgg gtgcttggcc
     1561 tctgcttcgg ggacgccgac gcgcgccgcc tcgtccgccg gcacgcccgg cgtcgaactc
     1621 gttcaaggag cgccgcccaa gctcgtgcac cgcttggagc acgcatgctg tctgcactcg
     1681 cagacacgca tactgcgccg cgaccgcgcg atccaggtca cggagcagct ctcgggcacg
     1741 ccctgccgct gcaagtgctc gtccacgttg agcaccgagc ttcgcgtgcc tgctggaagc
     1801 tacgagatct cgctcgtgct ggagcagggt ggaactcgca gcgaagtgta ccgcggtgcc
     1861 ctggacgtgg ccgcgagcag cggcggcagc gtcgcgtcgc caaccacggc gggctcacag
     1921 ctcccgcgaa cgattcacgt ccctgctgcc aagcactaaa aaacgccgcg caagcctcag
     1981 gcattactcc tcaggcagta ctggagccgg aacgagaccc tgggcccgcg ctcgggcgtt
     2041 tgacaatgct ttcgatcagc tcttgcatgt ctgccggagg cgcatcgagg tgaatcccca
     2101 accgatacga ccagaccaag ccctcggtcg gtgaaatctt ctcggtacgc accaccgtgg
     2161 catcgagaac gcgcggctcc gcctcgcgcg gaacgtacag cctgagcagc aacttgctgc
     2221 caaccggcgg ctctgcagcg gtgagcagca gcaatccgtt cccgctcgca ttgcgcgaca
     2281 cgccgcatcc caagcgctcc ccgtcgagct cgatttcggc gacgaagtgg accggctctc
     2341 ggggggcaac gcgcttgtcg ttcatatcga ccatcaacca atcaatctac aacggcccgc
     2401 gcgctctgtt cgcgaaaatt tactcgagct cacaaaacgg cgcgagcaac gacgaatatc
     2461 gctgctcgcg ctcgttttga ggcaaaaaga ctcagttcgc gaggcgttca gggcctcggc
     2521 ctcgaagttc agcgattcgc ccgctcgcgc gcttcgccct cgagctcttt taccttgccc
     2581 ttggcctgaa gctcgtcatc gccaatgagc gcgccgacgc ggttcttgat ggccccgacc
     2641 gcttgctggg ccttgcccgt agcgcgctcc gcggccttcg cggattcctg cttggcttgg
     2701 ccctcgagct ttcgcgcgcg gccttcggct tccattcgct cgctaccgac gaccttgcct
     2761 acgacctctt tgatcttgcc gccggtttct tccgctgcgc cttcgctgcg cttacttgca
     2821 ttgctcatgt tcgatctcct ctgaaagcgg atgcctgacg gcattcgcga tcgtctgcga
     2881 catcacagtg gcaaggtgca tgccatgagc gtcgagctga ccaatgcatg gaacgccttc
     2941 gtgaaacccc gcgcgcgcga cctggttcgt ggggcgattg cgacagcttt cgacgacact
     3001 gcgtgcctca gttctcggtg gttgccgctt tcccgccgcg ttcgaggcga aattggagga
     3061 cacgtgctgc atcgaacctt ccggtccagc cgcaccaaag caatggtgct cgtctgcgtt
     3121 cgcacctgag cgcacgcaac tcgcgaggcg attgctcggt tgcgaacgca cctacaatgc
     3181 gaccagttcg acggctcggc gtactcgcct tggttcgttg gggcggcgcg cgcggccctc
     3241 ttccgaaatg aattcggaga gactccgacg cggggacttc gtgcggagcc gttctccgct
     3301 accttcagcg aattcctcgc caagcaactc gaagatcgcg ccgggaggcg ctcgtccagc
     3361 tcgattagag ggt
//
LOCUS       DASTKS010000279         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1829865, whole genome shotgun sequence.
ACCESSION   DASTKS010000279 DASTKS010000000
VERSION     DASTKS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829865"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFQ35_05745"
     CDS             <1..654
                     /locus_tag="VFQ35_05745"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET9930164.1"
                     /translation="PIFHSRFVEYARVRGFAIFAAQPRRPTDKPFVERGIGFLRTRFL
                     PGRRFASLQQLAREATAWRDTFANAREHEQTGKVPALVFEHEERQTLAPLRTQTFDTD
                     ELLPTSVGRTHRVHFDRNDYTVPWRLVGQSVLVRADDELVRVMLGPKEVARHVRSWSV
                     REDVEKRTTRMVCGLSATRITLGRCRTHCHPWAIPACATLLYSQLLAAQSAQSKYDS"
     gene            651..923
                     /locus_tag="VFQ35_05750"
     CDS             651..923
                     /locus_tag="VFQ35_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930165.1"
                     /translation="MMLSELFGSRATASAIDEVMRTGHVGAEYVEYVMRHKRKLRPQA
                     APLRLGVPALDSITVSEPDLGRYDEICTGRPVRDPGTPRDNEEPEP"
     gene            920..1672
                     /locus_tag="VFQ35_05755"
     CDS             920..1672
                     /locus_tag="VFQ35_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930166.1"
                     /translation="MKAELDLSAVIDKLRWLKLPGMARGVEALVARATKNNMSPLELI
                     DALAEEERVSRIKSAVERRIRGARFAEINTVDAFDFDWDPTRKKMRAKYLALHDLGFL
                     DRAVCPIFVGSPGTGKTFLARALAFRACQATKNVLFTSARMLNDLAGAEVHGQLTRTL
                     RKYTSPALLVVDDFAVLEMDTAQAKLAFQVISERYDQRRSTAITTNRSFKDWTKVFPD
                     PLNAQIIAERLTERSEVFLLDGKSYRDPRNKT"
BASE COUNT          419 a          727 c          671 g          406 t
ORIGIN      
        1 cctatctttc actcgcgctt tgtggagtac gcccgcgtgc ggggcttcgc aattttcgca
       61 gcccagccga ggcggcctac cgacaagcct ttcgtcgagc gcgggatcgg ttttctgaga
      121 acccgattct tgccaggtcg gcgcttcgcg agcctgcaac agctcgcacg ggaggcgaca
      181 gcctggcgcg acacattcgc caatgctcgc gagcacgagc agacgggcaa ggtgccggcg
      241 ctcgtgttcg agcacgagga gcgccagaca ctcgcgccgc tgcgcactca gactttcgat
      301 acagacgaac tgctgccgac gagcgtcggc cgcacacacc gcgtccattt cgatcgcaac
      361 gattacacgg ttccgtggcg cttggtgggg caatccgtgc tcgtacgcgc cgacgatgag
      421 ctcgtgcgcg tgatgctcgg cccaaaagaa gttgcacggc acgtgcgctc gtggtcggtg
      481 cgcgaagacg ttgaaaaaag aaccacgagg atggtatgcg ggctgagcgc gacccgcatc
      541 acgctggggc gctgccggac gcactgtcac ccttgggcga taccggcgtg cgctactttg
      601 ctctactcgc agcttctcgc cgctcaatcc gcacagagca agtacgactc gtgatgctca
      661 gtgagctctt tggaagtcgg gcgaccgcga gcgccatcga tgaagtcatg cgcacggggc
      721 acgtcggcgc tgagtacgtc gaatacgtca tgcgccacaa acgcaagttg cggccgcagg
      781 ctgcaccgct tcggctgggc gttccggccc tcgattcgat cacggtgtcc gagcctgacc
      841 tcggccgcta cgacgagatc tgtaccggtc gcccggtgcg cgacccaggt acaccgcgcg
      901 acaacgagga gcccgaaccg tgaaagccga acttgatctg agcgcggtga tcgacaagct
      961 ccgctggctc aaactgccgg gcatggcgcg cggggtcgaa gcgttggtcg ctcgtgcaac
     1021 gaagaacaac atgtcgccgc tcgagttgat cgacgcgctt gcagaagagg agcgcgtcag
     1081 tcgcatcaag agtgcggtcg agcgacgcat tcgcggtgcg cgattcgccg agatcaacac
     1141 ggtcgatgcg ttcgactttg attgggaccc gacacggaaa aagatgcggg cgaaatacct
     1201 ggccctgcac gacctcgggt ttctcgaccg cgccgtctgt ccgatcttcg tcggctctcc
     1261 gggtactggg aaaaccttcc tcgcgcgcgc tttggcgttt cgtgcctgtc aggcgaccaa
     1321 gaacgtcttg ttcaccagcg ctcgcatgct caacgacttg gccggtgccg aggtacatgg
     1381 tcagctgacg cgcacacttc gcaagtacac ctcgccggcg ttgctcgtcg tcgacgactt
     1441 cgccgtgctc gagatggaca cggcacaagc caagctggcg tttcaggtga tctccgagcg
     1501 ctacgaccaa cggcgctcga ctgcgatcac gaccaatcgc tcgttcaagg attggaccaa
     1561 ggtctttccc gatccactca acgctcaaat catcgccgaa cgactcacgg aacgcagcga
     1621 ggtcttctta ctcgacggca agagctatcg agacccccgc aacaaaacct gatcctcaaa
     1681 cgaaccgaac cggcgggtct catcccggaa ctcgcgctga cccaagcgct cgaggcagag
     1741 cgtgcttctg cggcgcgaac tcgatcgcac agctccgtcc gcaccgccac cggtggggcc
     1801 catggcgccg gtgatctcgg tgattttggt accttcgctc gccggtggcg acaatgaacg
     1861 ccctctgggt ctccaacact ttggccggca aggttaccta gcgcgcgttc tgcgaacggc
     1921 ggtcgctccg ccggcctctc cctcttcccc cgcgcgccgc gcggaagccg aaggcttact
     1981 tctgcacgtt gcggcgcaag gcttaaccgc gccgcggctc aggtgtcgaa gcaattcgag
     2041 aaagtccaag tcctcgcgca gatccgcggg attagtgccc gcagatgagc atcgcctggt
     2101 cctgccaaat gcccttcacg ccgccaccga ccgtacactc ggctccgtgc aagttgtggt
     2161 cggcgtccat gcacgtacaa gtcaacctgg tactcgatgg atcgtagttg caatccgtgc
     2221 agc
//
LOCUS       DASTKS010000280         6833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_491480, whole genome shotgun sequence.
ACCESSION   DASTKS010000280 DASTKS010000000
VERSION     DASTKS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491480"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1191)
                     /gene="ttcA"
                     /locus_tag="VFQ35_05760"
     CDS             complement(352..1191)
                     /gene="ttcA"
                     /locus_tag="VFQ35_05760"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET9930167.1"
                     /translation="MCHTARRIMDRLEKRLLDGVARASADFALLEPNDRVLVAVSGGK
                     DSHALLYLLREIQRRAPFEFSLFALNVDQGHPGFPKQVLPEYFAREGYEYRIIEEDTY
                     SVVKEKVPAGKTTCSLCSRLRRGILYTAAQELGATKIALGHHRDDVIETLMLNLFFSG
                     QMKAMPPRLRSDDGRNVVIRPLVYAAERDLAEFAENKGFPIIPCDLCGSQEQLQRKQV
                     KALLAELEQKNPYLRANIFAALGNVRPTHLFDTDLYEKLGLREAVEAPEPSAAPLIAL
                     GRR"
     gene            complement(1445..2059)
                     /locus_tag="VFQ35_05765"
     CDS             complement(1445..2059)
                     /locus_tag="VFQ35_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930168.1"
                     /translation="MQNQTASWARLSVRWLGVVCALSLLGACHDEEAEKKKIAEIQKE
                     ADEKIKSIQKQADQKVADAEKQVEQVKQEMAAATQKVKAEADDAVSKAQASAEEQAKA
                     AQAALAKARGAFKEEGRLQLANVNKEVTELAQKSAKASAKVKEEVAKAMKQIVAKQKD
                     IAKDIAAFDKAELADMKKAQAKLNQDLALMKNIVRAAKAKVPAS"
     gene            complement(2180..2947)
                     /gene="trpA"
                     /locus_tag="VFQ35_05770"
     CDS             complement(2180..2947)
                     /gene="trpA"
                     /locus_tag="VFQ35_05770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9930169.1"
                     /translation="MNLADYIFERKKQRDILLMTHIVVGYPSLAASLELVDAMVEAGV
                     DLMELQIPFSEPIADGPVILHANQEALKLGIKVNDCFEFAAKVTRRHSIPFLFMSYYN
                     VLFRRGVATFAARSAEVGVRGAIVPDCPPEEATEYVDAMRKHELAPVFIFSPRTPTER
                     LALLGKAGDGFVYAVARKGVTGQDTEFSDDLAQYLARCRAATSLPLAVGFGLRSPKDV
                     DFLRSKADIAVVGTESLRLLEKSGVRAVGNFLATLRG"
     gene            complement(2934..4169)
                     /gene="trpB"
                     /locus_tag="VFQ35_05775"
     CDS             complement(2934..4169)
                     /gene="trpB"
                     /locus_tag="VFQ35_05775"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9930170.1"
                     /translation="MTEKPGYFSGFGGAFIPEILAATFSELERAFEAAKKDPAFWAEY
                     EALMSSYSCRPTPITFAENLSNHFGGARIYVKREDLNHTGAHKANNVMGQGLLVKRMG
                     KKRVIAETGAGQHGVATATMAAKFGFECTIYMGAEDVERQRPNVFWMERLGAKVVPVT
                     EGSCTLKDAINEAMRDWVSSMDTTHYVLGTACGPHPFPEMVAWFQSIVGKEARGQMQK
                     LIGRAPDRVYACVGGGSNAMGVFAGFLDNPSVELVGVEAGGHGLESGKHAARLASADA
                     SIGVAQGYRTYFLQDEDGQMRDTHSVAAGLDYVGVSPILSHYKHTGRVRFEAATDREV
                     IDALALAVRKEGLIPALESSHAFAQAFKEAPTLGTDKVLLINQSGRGDKDIFTVADAF
                     DDPAWKEFIKKKAASYESR"
     gene            complement(4166..4942)
                     /locus_tag="VFQ35_05780"
     CDS             complement(4166..4942)
                     /locus_tag="VFQ35_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9930171.1"
                     /translation="MVEATFATPLYAQIARRLRAQIQSGTYPAGCKIPSEHELAAHFH
                     VGRPTVRQATQLLVDERVLERRRGSGTYVSGAPREVDLFSAGGTLASFTRSGFALETR
                     VVAPARLVTTPSEIERLAGRAAFFVERTSLVDGEPVLLERMYFDAELFRGLDRMNLEG
                     RSLSEIARAEYMLEPIAAEQRFHVTSLDEAGAQRLALPIGTHVLKVQRIIDFRTASAA
                     VVAELYCNTLRLQFTQRLAFGRGGSVDLTSLAGPLAARSA"
     gene            5170..5670
                     /locus_tag="VFQ35_05785"
     CDS             5170..5670
                     /locus_tag="VFQ35_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930172.1"
                     /translation="MAADTSRHHAIKELTDTLSQLLDREVQINAAASGEPTEIVVRYV
                     DGSGDLVAAWTVERVLSWSLGAALTMVPRGMVDEAIKNGSADAQIDENFKEVVNVLAT
                     AAANVVARRAVLESITEEAPTLMRTARQISAAGGHWTVLEVNVDGYPPGRMAVSFAPP
                     AQADAE"
     gene            complement(5791..6621)
                     /locus_tag="VFQ35_05790"
     CDS             complement(5791..6621)
                     /locus_tag="VFQ35_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET9930173.1"
                     /translation="MAITIQTNISSLETQKALNKTQSALNKNFTKLSSGYRINTAADD
                     AAGLAISESMKMQIRSYVVAERNANDALSMTQTAESVLGEMSDILGRMRELAMQGANG
                     SMTDVDRGYLDTEFTALKEEISRMQSSAKFNGRQLIAQTAASIIFQVGLNNTASDQIQ
                     LTFGGLGLTTLLDATTNVAGTTAANALSALGRIDSALQTISTNRAKYGATMNRLEVSI
                     SSIQTMRLNLTAANSRIRDVDVAQETAELSRNQILSQAGSSVLAQANQLPQLALQLLK
                     "
BASE COUNT         1141 a         2081 c         2286 g         1325 t
ORIGIN      
        1 tgaaacggac gacaacacga agctcgtaaa gagcacgcaa gaacgaacac tctttctcgt
       61 catgggggta acgctccctt cgacgggggg tggtgcaagg gccgttccca ggtgtttcgc
      121 cgcgattcgc gcacgattca cgcggtcttc gtggaagcgc gtcgaggagt gtcgacgaac
      181 gtcgatgaat gtcggagccg cgcaatcgac ttggcaaacg ctttacgttg gcgcgatcgc
      241 tagcgtgatc acgaaactcg aacccatcgt cgcatcgacg cagcgcagag ctccgctgta
      301 acgcagaacg agctcacgcg atacgagcgg cgaagctcgc gcgttccgga gctatcggcg
      361 acccaaggcg atgagcgggg ccgcgctcgg ctccggagct tctaccgctt cgcgaaggcc
      421 gagcttttcg tagagatcgg tgtcgaacaa gtgggtgggc cgcacgttgc cgagcgccgc
      481 gaagatattg gcgcgcagat aggggttctt ttgctcgagc tcggcgagca gcgccttcac
      541 ttgcttgcgc tggagctgct cctgagagcc gcacaggtcg cagggaatga tgggaaatcc
      601 cttgttctct gcgaactcgg cgagatcgcg ctcggccgcg tacacgagcg gacggatcac
      661 cacgttgcgc ccatcgtccg agcgcaggcg cggcggcatc gccttcattt ggccggagaa
      721 gaacaagttc agcatcaaag tctcgatgac gtcgtcgcga tgatggccga gcgcgatctt
      781 ggtcgcgccg agctcctgcg cggcggtgta caggatgccg cggcgcaaac gcgaacacag
      841 cgagcacgtc gtcttgccgg cggggacctt ctctttgacg acagagtaag tgtcctcctc
      901 gatgatgcgg tactcgtagc cttcgcgagc gaagtactcc ggcagcactt gcttcggaaa
      961 gccggggtgg ccctggtcga cgttgagcgc gaagagcgaa aactcgaacg gtgcgcggcg
     1021 ctgaatttcg cgtagcaagt agagcagcgc gtgcgaatct ttcccgccgc tcacggcgac
     1081 gagaacgcgg tcgttcggct cgagcagagc aaaatcggcg ctggcccgtg cgacgccgtc
     1141 tagcaggcgc ttttccaagc ggtccatgat gcggcgcgca gtatggcaca gcttttcggc
     1201 gcggcacttt tccccgaatg agccgcattt cggagtgaat cgcgccgttt aggcagcgct
     1261 ccacgtgctt tgggcgatga gcgcgcggtt cacgtcgaag cggcgcggtc cacgtggggt
     1321 cggcgccgtt cacgacgggg cggggcgggc gcggccctgg acccgctcgc cgggcgcagc
     1381 gaaggcctac cgcgcccggc gccagagttg cgcgctcttt acggcgcgta ccccaaacct
     1441 gcgatcagga agcggggact ttggcctttg cggcgcggac gatgttcttc atcagcgcga
     1501 gatcttgatt gagcttggct tgcgccttct tcatgtcggc gagctcggcc ttgtcgaacg
     1561 cggcgatgtc tttggcgatg tccttctgct tggccacgat ttgcttcatc gccttggcca
     1621 cttcttcttt gaccttggcc gaagctttgg cgctcttttg cgcgagctcg gtgacctctt
     1681 tgttcacgtt ggcgagctgc aggcggccct cttctttgaa ggcgccgcgc gccttggcga
     1741 gggcggcctg ggcggctttg gcttgctctt cggcgctcgc ttgggccttg ctgacggcgt
     1801 cgtcggcctc cgccttcacc ttttgggtgg cggcggccat ctcttgtttg acttgctcga
     1861 cctgcttttc ggcgtcggcg accttttggt cggcctgttt ctggatgctc tttatcttct
     1921 cgtcggcttc tttctggatt tcggcgatct tctttttctc tgcttcttcg tcgtggcacg
     1981 cgccgaggag ggagagggcg caaactacgc cgagccagcg aacagacaga cgggcccacg
     2041 aagccgtttg gttctgcatc gcagaaacgt tagggcagtc tggcgacgag ttcaacttcg
     2101 ggttttggtg agcgccggga gtcgtgacgg gtcgctcgcg cggtgattgc gcgaacatgc
     2161 gagtttcgcg taggccgacc tacccgcgga gtgtcgccaa gaaatttcct acggcgcgga
     2221 caccggattt ttcgagcaat cgcaagctct cggtgccgac gacggcgatg tcggctttgc
     2281 tgcgcagaaa gtcgacgtcc ttcggcgagc gtaacccgaa gccgaccgcg agcggtagcg
     2341 aagtcgcggc gcggcacctc gcgagatact gcgcgaggtc atcggaaaat tccgtatctt
     2401 gaccggtgac gcctttccgc gcgaccgcgt aaacgaagcc gtcgccagcc ttgccgagca
     2461 gcgcaagtcg ctccgtggga gtgcgcggag agaagatgaa gacgggtgcg agctcgtgtt
     2521 tgcgcatcgc gtccacgtac tcggttgctt cttcgggcgg gcagtcgggc acgatcgcgc
     2581 cgcgcacgcc gacctccgcg ctgcgcgcgg cgaaggtcgc gacgccgcgg cggaagagca
     2641 cgttgtagta gctcatgaag aggaacggaa tcgagtggcg ccgagtcacc ttcgccgcga
     2701 actcgaagca gtcgttcacc ttgatgccga gtttcaacgc ctcttgattg gcgtgcagaa
     2761 tgacgggtcc gtctgcgatt ggctcggaga aggggatttg cagctccatc aagtcgacgc
     2821 cggcttcgac catggcgtcc acgagctcga ggctcgccgc gaggctcggg tagccgacga
     2881 cgatgtgcgt catcagcaag atgtcgcgct gctttttgcg ctcgaagatg tagtcagcga
     2941 gattcatagc ttgccgcttt cttcttgatg aactctttcc aggccggatc gtcgaacgcg
     3001 tccgcgaccg tgaagatgtc tttgtcgccg cgacccgatt ggttgatgag cagcactttg
     3061 tcggtgccga gcgtgggcgc ttctttgaaa gcttgagcaa aggcgtgcga tgactcgagc
     3121 gcggggatga ggccctcttt gcggacggcg agggcgagcg cgtcgatcac ttcgcgatcc
     3181 gtcgcggcct cgaagcgcac ccgaccggtg tgcttgtagt gcgagaggat gggcgagacg
     3241 ccgacgtagt cgagccccgc cgcgacgctg tgcgtgtcgc gcatttggcc gtcttcgtct
     3301 tgtaggaagt aggtgcggta gccttgcgcg acgccgatgg acgcatcggc gctcgcgagg
     3361 cgcgcggcgt gcttgcccga ctcgaggccg tggccgcccg cctcgacccc gacgagctcg
     3421 acgctcggat tgtcgaggaa cccggcaaag acccccatcg cgttcgagcc gccaccgacg
     3481 caggcgtaga cgcgatcggg agcgcgcccg atgagctttt gcatttggcc acgcgcctct
     3541 ttcccgacga tcgactggaa ccacgcgacc atttcgggga aggggtgggg gccgcaagcg
     3601 gtaccgagca cgtagtgcgt cgtgtccatg ctgctcaccc aatcgcgcat ggcctcgttg
     3661 atcgcgtctt tcaacgtgca cgagccctcg gtgacgggga cgactttcgc cccgaggcgc
     3721 tccatccaaa acacgttggg gcgctggcgc tcgacgtctt cggcgcccat gtagatggtg
     3781 cactcgaagc cgaacttggc ggccatcgtc gcggtcgcga cgccgtgctg gccggcgccg
     3841 gtctcggcga tgacgcgctt tttacccatg cgcttcacga gcaggccctg acccatcacg
     3901 ttgttggctt tgtgggcgcc ggtgtggttg aggtcctcgc gcttcacgta gatgcgcgcg
     3961 ccgccgaagt ggttgctgag gttttcggcg aaggtgatcg gcgtcggacg acaggaatac
     4021 gagctcatca gcgcctcgta ttcggcccaa aacgcaggat ccttcttggc ggcttcgaat
     4081 gcgcgctcga gctcgctgaa cgtagcggcc aagatctcgg ggatgaacgc gccgccgaaa
     4141 cctgaaaagt agcccggttt ttccgtcatg ctgacctcgc ggcgaggggc ccggcgagcg
     4201 acgtgagatc gacgctgccg ccgcgcccga atgccaagcg ttgggtgaat tggagtcgaa
     4261 gggtgttgca gtagagctcc gcgacgacgg cagcgctcgc ggttcgaaag tctatgatgc
     4321 gttggacctt gagcacgtgc gtaccgatcg gcagagcgag gcgctgagcg cccgcttcgt
     4381 cgaggctcgt gacgtggaag cgctgctcgg cggcgatcgg ctccagcatg tattctgcgc
     4441 gcgcgatctc cgacagtgag cgcccttcga ggttcattcg atcgaggccc cggaagagct
     4501 cggcatcgaa gtacattcgc tcgagcagca ctggctcacc gtcgaccaag ctcgtgcgct
     4561 cgacgaagaa cgcggcgcga ccagcgagcc gctcgatctc gctcggcgtg gtcacgagtc
     4621 gggcgggagc gaccacccgc gtttcgagcg cgaagccgct gcgcgtgaac gacgccaacg
     4681 ttcctcccgc cgaaaagaga tccacctcgc gcggcgcgcc actgacgtag gttcccgagc
     4741 cgcgtcgccg ctcgagcacc cgctcgtcga ccaacagctg ggtcgcctgg cgtacggtcg
     4801 gacgccccac gtgaaagtgc gccgcgagct cgtgttccga cgggatcttg cagccggccg
     4861 gatacgtccc cgattggatc tgcgcccgca gccgtcgcgc tatttgcgcg taaagcggcg
     4921 ttgcaaaggt ggcttcgacc atcgggacag caaagctgat cgaaatctgt aaagttgtca
     4981 agacaactgt ccgaggcggc ggggagtcgg gcctcgaaac tagggaaaac ctctggcggc
     5041 gcttggagga attccagttg tgctgcgaag aggtcgtgct caggcggtcc gccggcagcc
     5101 tgaatttcag cgcgcagctg ccgttcgatt gggggcaaga ccggatttca cggttcgcgt
     5161 cccgttccca tggcagccga tacaagtcga caccacgcaa tcaaggagct caccgacacg
     5221 ctctcgcagc tcctcgaccg tgaggtgcaa atcaacgcgg ccgcgtccgg tgaacccacc
     5281 gagatcgtcg tgcgctacgt ggacggatcc ggagatctgg tcgcggcgtg gacggtcgaa
     5341 cgggtgctct cgtggagcct cggtgcggcg ctcacgatgg tgccgcgcgg catggtcgac
     5401 gaggccatca agaacggcag cgccgacgct cagatcgacg agaacttcaa agaggtggtg
     5461 aacgtgctcg ctacggcggc ggccaacgtg gtcgcgcgac gcgccgtgct cgagtcaatc
     5521 accgaggaag cgccgacctt gatgcgcacc gcgcggcaga tcagtgccgc gggcgggcac
     5581 tggacggtgc tcgaagtgaa cgtcgatgga tatcctcccg ggcgtatggc cgtgtcgttt
     5641 gcaccgccgg ctcaggccga cgcggagtga agcgccgcgt cggtcgcgcg actcgcgggc
     5701 gcacccgacg agtgaggcct ttccttcgac tttctcggga tggagtcacg agagaaatcc
     5761 cctcgaattc gccgcgaatt gccgggaggc tcacttgagc aactgcaggg cgagctgcgg
     5821 caactggttc gcttgcgcca acaccgagga gccagcttgc gagaggattt gattgcggct
     5881 gagctccgcc gtctcttgtg ccacgtcgac gtcacggatg cgcgagttcg cggcggtgag
     5941 gttcaaccgc atcgtctgga tcgacgagat cgagacctcg aggcggttca tcgtcgcgcc
     6001 gtacttcgcg cggttcgtgg aaatggtctg gagcgccgaa tcgatgcgcc cgagcgcgct
     6061 cagcgcgttg gcggcggtgg tgccggcgac gttggtcgtc gcgtcgagca gcgtggtgag
     6121 gccgagcccg ccgaacgtga gctgaatctg gtcgctcgcg gtgttgttca gcccgacctg
     6181 gaagatgatg ctcgccgccg tctgcgcgat gagctgcctg ccgttgaact tggccgagct
     6241 ctgcatgcgc gagatttcct ctttcagcgc cgtaaactcg gtgtcgaggt agccgcgatc
     6301 cacgtcggtc atgctgccgt tcgcgccttg catcgcgagc tctcgcatgc ggcccaagat
     6361 gtcgctcatc tcgccgagca cgctctccgc ggtctgcgtc atcgacagcg cgtcgttcgc
     6421 gttgcgctcg gcgaccacgt acgaacgaat ctgcatcttc atgctctcgc tgatggcgag
     6481 ccccgcagcg tcgtcggcgg cggtgttgat tcggtagccg ctcgagagct tggtgaagtt
     6541 cttgttcaag gccgattggg tcttgttcag agccttctgc gtctcgagcg aggagatgtt
     6601 cgtttgaatc gtgatcgcca tggtcgtctt ctgggtgggc gggcctgagc gacgacagga
     6661 actgcaggag ccatgccacc cagtaacgtc atggaaacca gcgatgcgcc gtcgagcggc
     6721 gaccgcgccc gcacacggac cctgtcagcg ggttgacccc gaggtgccaa actaaccgaa
     6781 ggcacccctc tgaagacgcg gcgctgtcag ccgcgaatgg ctttgacggg ctc
//
LOCUS       DASTKS010000281         9760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1383892, whole genome shotgun sequence.
ACCESSION   DASTKS010000281 DASTKS010000000
VERSION     DASTKS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383892"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFQ35_05795"
     CDS             <1..233
                     /locus_tag="VFQ35_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930174.1"
                     /translation="ADLSPKALEMFYRLYVWQEEPEVVARETGSKLEAIYQWRSRLKK
                     EVSQLELKLSAPPAPGLHLRGPSNLSKSNASS"
     gene            220..972
                     /locus_tag="VFQ35_05800"
     CDS             220..972
                     /locus_tag="VFQ35_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930175.1"
                     /translation="MPHPEDLVDDVAQVARDEEALGAASERKLAGTASAADEALLANM
                     PEFLTAPLSEQRRGAVVDAIHARIANSERPKSLLVGVPSRAQRTPRGARLGVALMGAT
                     AMAAGIFVWLARAPNRGEPLSGYELRVVGALRSERSAAPATETLRLRPGTTVRFELRP
                     QRDVRGDVAARVFVGGAASQALGELTTEQEQSTAGALRVDARLPDSLPAQGELLIDVG
                     RRDALARVQPSTAFARPSLETQQFRVTFERTP"
     gene            987..3704
                     /locus_tag="VFQ35_05805"
     CDS             987..3704
                     /locus_tag="VFQ35_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET9930176.1"
                     /translation="MNEARWLRRFALLTLMGSLALSDGSGDASVGGLEFEFAGCARVR
                     PGPVCELAPERDLVLWTAGTEPPRIEGEDAAAPELLESAQVEGGVRLRVRVAAGERGI
                     RLRKRHGYAALRVAPDSESDELRELARLWKAGKWDAVRARLSAPEPSGNGPERERKRA
                     FAARAALRAGDNERAATELEATAQSALRAGSLLDAGNDLVAAAYCRAVRLREYDKARR
                     LLESARELEGVPEIRAWLTYYRGVLANATGDARRALVEFRRASVLARRLGLLSYEVLA
                     RQELAITLNRLHRHAEALVQQRELVALNLDAPSCLHSQRWEKLAWLLLSQPEPNLEEA
                     AAALNRAEELLVDCPDPVSLRNQAMNRVELDLKLGNTLAARARLGALAADPSGRNPEL
                     SAWQERYFGELQLAEGAVDEAIRSFGRSEELAESVDLKSCVYSARLGRARALAKRSDE
                     AAIAAYGAAEDAADALVKWAPFGQGQQLTASEVQQSSRELLSLLLAQGRSEEAFSVAA
                     RALQRAWTSGAHSSRVATLASPLRQRWERAVAEYRAIRDELERVSSDDWKLSLDGLMA
                     IRLSRALDRQRLDNALATASALIARPPPTTRYRSEQDAQLLLAKGRDEWWAMLSTPSG
                     LHAAKVAPVEARAGPVTRDLAPRLARVLEEFVRAGYLNVPLLRVTLPPELAELDVHAL
                     EVAGQPLIERVAVAYAFGPASESKSHATFMNSTIVVLGNPNGDLPWAETEAREVAGRL
                     GAPPALLRGDVTLAAVTARLSGAGLLHFAGHGRAGGIDGIESALLLSAEQRLALGDVL
                     ALERVPDFVVLAACESTVSPEPGGAISIGQAFLLAGARAVVGASRVVSDRVSQRFARA
                     LYPRLSALGGTRALSSDVHVWAGAVRAAALDLREYDPHLDWASMRLLLR"
     gene            3965..6271
                     /locus_tag="VFQ35_05810"
     CDS             3965..6271
                     /locus_tag="VFQ35_05810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HET9930177.1"
                     /translation="MRPKAIRLQGIDVKFSHPGARLLLPTLVVLLPCVFSCGSRAESA
                     DAPERAVALLASADGTPGSGGACSDFALSAPRTVDLGQSFAATAMRRLLGRQTWVVAA
                     NNTAYSWDGEGFGIAAQGVRASVDCTWSGSHTLEVTRASDGCKATALVRCEPPLCSGT
                     SGPCPGVPTKGTTPPTTNLLVRIREGLSAPPGQFGTDAVCPAEIGAWVGGPLLRVRDE
                     QGDATLYCSYAWVASPEYSPNVDAITQRLGTDVQWDEPNVAAHGEYAEMHAKLASQSR
                     AQLGVVSWPPAPAPEPVRIAVVDTAANFWRDPDNNPHGVAVGALALDTACKDSPSCKV
                     SAENFLALPLYRDTETSPAVVRRDVLHGGSFGAHGDFARAIRDASNAAPVNARTIINL
                     SVAYDSDELVDGVKVKPTDYSNQVVLDALQYAHCRGALIVAAAGNGPVPAQSDQLAGL
                     PARWTAVHALPASECQARFGIANPRPGNGTGPLLYAISGLDFGANPLLTTRGSGQSAL
                     AAVAFAAVREVPNGGYTRTLTGSSMSAASVSGIAAALWSHAPSLTPDAVIAQLYAASE
                     PVAADADFVPYAPSGRDNGFAAVRRITLCSAATAGYAVCVEPPVPSPVVAKGTVPPLP
                     AGSAVEIGKTLESSVAGPSPWKYPWLFPQPEGEPGCGACALSLRDRGVLSLGFRSSFP
                     LSMISNMRVRVRVPIVAASFALQGDESVDESLTMLKESAVPNLSLDPFTVTLGYSTIG
                     ATAAELVYVVAEDDVSVSVEESESVLLQ"
     gene            complement(6319..6756)
                     /locus_tag="VFQ35_05815"
     CDS             complement(6319..6756)
                     /locus_tag="VFQ35_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation/crystallin J1"
                     /protein_id="HET9930178.1"
                     /translation="MILYRPVGIGELRLIAQSAFSAFPPRLPDQAIFYPVLTHEYARR
                     IARDWNTVDAQSGYAGFVTRFEIDDVTAARYPVQRAGGRTHEELWVPAAELAEFNAHI
                     VGRIAVIEAFTGERFAGALNPATLLPLELELELRAAAGAQSRT"
     gene            complement(6967..7407)
                     /locus_tag="VFQ35_05820"
     CDS             complement(6967..7407)
                     /locus_tag="VFQ35_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9930179.1"
                     /translation="MTLAPHVENCDTVRMQSPVEIAVETYIRAVGERDPNARAALIRA
                     CFAEDGRMVTRGREIRGHAALVDEIARFVADPRVLRVRMTSAVDACGTIFRYRAVAEF
                     RDGTSAESFDAGEIDSMGRISLLLTFTGPLADASEERIVLDLST"
     gene            7747..7962
                     /locus_tag="VFQ35_05825"
     CDS             7747..7962
                     /locus_tag="VFQ35_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930180.1"
                     /translation="MIRANHPTWEKLVKGEVKHKFAAASASMLFFNLQSQYKRDPSKL
                     PDLVGEAQKFFEKYEVSLETDIATLFK"
     gene            7977..8948
                     /locus_tag="VFQ35_05830"
     CDS             7977..8948
                     /locus_tag="VFQ35_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930181.1"
                     /translation="MLATTEQVEDLVSRGKSLILAGEEQLLASLPKGNWIGGTIPYFV
                     GAEGGRTTRDHIFVQPVADASTKCTIQVYDAKTISQVAVDSPEPGYTVLILPAFSQIH
                     QDYALNAPNYEQLFFKVVGGWIAGIHLDDLGKKSPKVFNGFTGETLASAGVAMHVELP
                     PKRQAQLGIVNIFEQGNGDELRFPKTGFSASECLVNGERRNLAEYYKTKGIDSRLPLV
                     ADFCGAMINVSIQAIDDAAGVVKFYAPVFDTAVYRVAKPVANYAKEFGGAIPTTIKNP
                     VFSCNCVLNYLHGELENRRTGSVTGPMTFGEIGYQLLNQTLVYITVP"
     gene            complement(9008..>9760)
                     /locus_tag="VFQ35_05835"
     CDS             complement(9008..>9760)
                     /locus_tag="VFQ35_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9930182.1"
                     /translation="APLIKFLEQIGSTLDLTLRSVTWLFRPPYRLSLFVQAMDFVGVQ
                     SIFIVGLTGVFSGMVLALQTTHSLRNFGAEGIVGSLVAISLTREISPVFAALMVTARA
                     GSAMAAELGNMRVTEQIDALVTMGVSPAQYLLSPRLLAATLMLPLLCILYTCVGMVGA
                     YVVAVQWLGVDPGVFINNIEKYLVPKDFWMGEIKAFVFGFLVSAISCAQGFNASGGAK
                     GVGEATTRAVVESAVAILVANYVLTSWLTDMG"
BASE COUNT         1719 a         3147 c         3239 g         1655 t
ORIGIN      
        1 acgctgatct ctcgccgaag gccctcgaga tgttttaccg cctctacgtt tggcaagaag
       61 agccggaggt cgtggcgcgt gaaacaggga gcaagctcga ggcgatttat caatggcgca
      121 gccggttgaa gaaggaggtc agtcaactgg agctcaagct ttcggccccg cccgcacccg
      181 gcctgcacct tcgagggccg tccaacctga gcaagtccaa tgcctcatcc tgaagatctc
      241 gtcgacgacg tcgcgcaggt cgcgcgcgat gaagaggcgc tcggggccgc ctccgagcgt
      301 aagctcgcgg ggacagccag cgcggcggac gaggccctgc tggccaatat gccggagttc
      361 ctgacggcgc cgctttcgga gcagcgccgc ggcgccgtcg tcgatgcgat tcacgctcgc
      421 atcgcgaact ccgagcggcc gaagagcctt ctcgtgggcg tgccgtcgcg cgcgcagcgc
      481 acaccgcggg gagctcggct cggagtcgcg ctgatgggag caaccgcgat ggcggcaggc
      541 attttcgttt ggcttgcgcg cgcccccaac cgcggtgagc cgctctcggg ttacgagctg
      601 cgggtcgtgg gcgcgcttcg gagcgagcgt tcggcggcac ccgcgactga aacgctgcgg
      661 cttcgtcctg gcacgacagt gcgattcgag ctcaggccgc agcgtgacgt tcgcggtgac
      721 gtcgctgcgc gcgtgttcgt gggcggcgcc gcgagccaag cgctcggcga gctcacgaca
      781 gagcaggaac aatcgacggc gggcgcgttg cgcgtcgacg cgcgcttgcc cgactcgctg
      841 ccagctcagg gcgagctctt gatcgacgtt ggccgtcgcg acgccctcgc tcgtgtgcag
      901 ccgagcaccg ctttcgcccg accctctctc gaaacgcaac aatttcgagt gactttcgag
      961 cgcacgccct aacccgtcga gcgccgatga acgaagctcg ttggctgcgg cgcttcgctc
     1021 tgctcacgct gatgggctcg ctcgcgctca gcgacggcag cggcgacgcg agcgtcggcg
     1081 gcctcgagtt cgagttcgct ggctgtgcgc gcgttcgccc ggggccagtc tgcgagcttg
     1141 cgcccgagcg cgacctcgta ctttggaccg cgggcacaga gccaccacgc atcgaaggcg
     1201 aggacgctgc cgctccggag ctcctcgagt cggcgcaggt cgaaggcggc gtacgtcttc
     1261 gcgttcgagt cgcagctggc gagaggggca tacggctacg aaagcgacat gggtatgcgg
     1321 cgctgcgcgt tgccccagac tcggagtctg acgagctccg agagctcgct cgcctgtgga
     1381 aagctgggaa gtgggacgcg gtgcgagcgc ggctgagcgc gcccgagccc tcgggaaatg
     1441 gccctgaacg ggagcgaaag cgggcgtttg cagcgcgcgc cgcgctgcgg gcaggcgaca
     1501 acgagcgcgc cgccaccgag ctcgaagcaa ctgcccaaag cgcactccgc gcaggctcat
     1561 tgctcgacgc gggcaacgac ctggtcgcgg cggcctactg tcgcgcggtt cgactgcgcg
     1621 agtacgacaa ggctcgaaga ttactcgaga gcgcgcgcga gctcgaagga gtgcccgaga
     1681 tccgcgcgtg gctcacgtac taccgagggg tgctcgcgaa cgccaccggc gacgcgcggc
     1741 gtgcgctcgt tgagttcaga cgagccagcg tgctcgcgcg gcgactcggg cttctatcgt
     1801 acgaggtcct ggcgcgacaa gagctcgcga tcacgctgaa ccggctgcac cgacacgccg
     1861 aagctctggt gcagcaaaga gagctagtcg cgctgaatct cgatgcgccg agctgcctgc
     1921 actcgcagcg ctgggagaag ctcgcttggc ttctcttgag tcagcccgag ccgaatctag
     1981 aggaggccgc ggcggcgctg aatcgcgccg aagagctgct cgtcgattgt ccggatccgg
     2041 tcagtttgcg caaccaagcc atgaatcggg tcgagctcga tttgaagctc ggaaatacgc
     2101 tcgccgcgag agctcgcctg ggtgcgctcg ccgccgaccc gagcggccgc aacccggagc
     2161 tttcggcttg gcaggagcgc tacttcgggg agcttcagtt ggcggaagga gccgtcgacg
     2221 aggcaattcg ttcgttcggg cgctcggagg aactggccga gagcgtcgat ctcaagagct
     2281 gcgtgtattc ggcgaggctc ggtcgcgcgc gcgctttggc caaacgaagc gacgaagccg
     2341 cgatcgcggc gtacggcgcc gcggaagacg ccgccgatgc cctcgtaaag tgggcacctt
     2401 tcggccaggg tcagcaactc accgcttccg aggtacagca aagttcccgc gagctactct
     2461 cgctgctgct cgctcagggc cgctccgaag aggcattcag cgtggctgct cgagccttgc
     2521 agcgggcatg gacgtcggga gcgcactcga gtcgcgtcgc cacgttggca tctccgctac
     2581 gccaacgttg ggagcgagcc gtggctgaat atcgcgcgat tcgcgacgag ctcgagcgcg
     2641 tgtcgagtga cgactggaag ctctcactcg acggattgat ggcgattcga ctgtcgcgag
     2701 cgctcgatcg gcagcggctc gataacgcgc tggcaaccgc gagcgcgctc atcgctcgcc
     2761 cgccaccgac cacgcgatat cgtagcgaac aggacgcgca gctcttgctc gccaagggcc
     2821 gcgacgagtg gtgggcgatg ctctcgactc cgagcgggct ccacgccgcg aaagttgccc
     2881 ccgtcgaggc gcgggccggt ccggtaacgc gagacctcgc tccgcgacta gcacgcgttc
     2941 tcgaggagtt cgtgcgtgcc ggctatctga acgtccctct cttgcgcgtc actcttccac
     3001 ctgagctcgc cgagctcgat gttcacgcgc tcgaagtcgc ggggcagccc ctcatcgagc
     3061 gagtcgccgt agcgtacgct ttcggacccg cgagcgaatc gaagagccac gcaaccttta
     3121 tgaactcgac gatcgtggtg ctcggcaacc cgaacggcga cctgccctgg gctgaaaccg
     3181 aggcgcggga ggtggccggg cgcctcggag ctccgccggc gttgttgcgc ggtgacgtca
     3241 cgttggcggc ggtcacggcc cgcctatcgg gcgccggctt gttgcacttc gcaggccacg
     3301 gccgcgccgg aggcatcgac ggcatcgaga gtgctctgct gttgagcgcc gagcaacgcc
     3361 tcgcgctcgg cgacgtcctc gcgctcgagc gtgttccgga tttcgtcgtt ctcgcggctt
     3421 gcgagagcac cgtctcgccg gaaccgggcg gcgcgatcag cattgggcag gcgttcctgc
     3481 tcgcgggagc gcgggcggtc gtcggagcga gccgcgtggt ttcggatcgc gtgagccagc
     3541 gcttcgcacg cgccctttac cctcggctct cggcgctcgg cggaactcgc gcgctctcga
     3601 gcgacgtgca tgtctgggcg ggcgcggtgc gtgcagccgc gctcgatctc agagagtacg
     3661 accctcacct cgattgggcg agtatgcgct tgctgctccg gtgaacgctg agtcgttcac
     3721 tcgcgagcat caaccaatct cacaacgcgg ccatcgccga aaatacttcg tcagcaaagg
     3781 ctgattgacg gcgaatcata cgagcgaccg attgcgtcga agattcgaga cagatcggtc
     3841 gcgctgtgtg ttcggttgca attcacccga cccgctcggg tataggtcga agcctcgaac
     3901 taggcgaaca gccatcgccg ttcaggcgac gcgctcggca ccgtgagcga gcgaaggaga
     3961 ttcgatgcgt ccgaaagcca ttcgcctgca agggatagac gtgaagttct ctcaccccgg
     4021 cgcacgcttg ctgttgccga cgttggtagt tctgttgcct tgtgtgtttt cttgcggctc
     4081 ccgcgctgaa agcgcagacg caccggaacg ggccgtcgcc ttgcttgcga gcgcggatgg
     4141 cacgccgggt agcggcgggg cgtgtagcga cttcgccttg agcgcaccga ggacggtcga
     4201 tttgggtcag tcgttcgcgg cgaccgcgat gcgtcgcttg ctcggccggc agacttgggt
     4261 ggtcgcggcg aacaacaccg cttactcttg ggacggcgag ggcttcggca tcgcggcgca
     4321 aggcgtgcgc gcgagcgtcg actgcacttg gtcgggctcg cacacgctcg aagtcacgcg
     4381 cgcgagcgac gggtgcaaag ccaccgcgct cgtccgttgt gaaccgcccc tctgtagcgg
     4441 gacttctggc ccctgccccg gcgttccgac gaaaggcacc acaccgccga cgaccaactt
     4501 actcgtgcgc attcgggagg ggctctccgc tccgccaggt caattcggaa cggatgcggt
     4561 gtgcccggcc gaaatcggag cttgggttgg aggcccgctc ctgcgcgtgc gtgacgagca
     4621 gggcgatgcg acgctgtact gcagctacgc gtgggtggcg tcaccggagt actcgccgaa
     4681 tgtcgacgcc atcacgcaaa ggctgggcac cgacgtgcaa tgggacgagc cgaacgtcgc
     4741 cgcgcacggc gagtacgcgg agatgcatgc caagctcgcg tcacaaagtc gagcgcagct
     4801 cggcgtcgtc agctggccgc cggcgcccgc cccggagccc gttcgcatcg cggtagtcga
     4861 cacggccgct aacttttggc gagatcccga caacaacccg cacggagtag cggtcggcgc
     4921 gctcgcgctg gatacggcct gcaaagactc accgagctgc aaagtcagcg ccgagaactt
     4981 cctcgcgctt ccgctctacc gcgacaccga gacgtcgccg gcggtggtgc ggcgtgacgt
     5041 cttgcacggc ggatccttcg gcgcgcacgg cgatttcgcg cgcgcgatcc gcgacgcaag
     5101 caacgcggcg ccggtgaacg ctcgcaccat catcaacctg agcgtcgcgt acgacagcga
     5161 cgaactggtc gacggagtga aggtgaagcc gaccgactac tcgaaccaag tcgtgctcga
     5221 cgctttgcag tacgcgcact gtcgcggtgc cctgatcgtg gccgcggccg gtaatggacc
     5281 cgtaccagcg caatcggatc aattggcggg gctccccgcg cgctggacgg ctgtgcacgc
     5341 actacccgca agcgaatgtc aggcccgttt cggcatcgcc aatccacggc ccggcaacgg
     5401 aacgggcccg ctcctctacg ccatcagcgg gctagatttc ggagcaaacc cgctgctcac
     5461 gacccggggg tcgggccagt ccgcgctcgc cgcggtcgcc tttgccgcgg ttcgcgaagt
     5521 gccgaacggc ggttacacgc gcacgctgac tggctcatcc atgagcgctg cctcggtctc
     5581 tggcatcgca gccgcgcttt ggagccacgc gccttctctc acacccgacg cagtcatcgc
     5641 ccagctttac gcggcgagtg agccggtcgc ggctgacgcc gacttcgttc cgtacgcacc
     5701 gagcggcaga gacaacggtt tcgccgctgt gcggcgcatc acgctgtgca gcgccgcgac
     5761 cgcggggtac gccgtatgcg tcgaaccgcc ggttccgtcg ccggtcgtcg cgaagggcac
     5821 cgtacctccg cttccagcgg gcagcgcggt cgagatcggg aagactctcg agagctcggt
     5881 cgctggcccg agtccgtgga agtatccgtg gctctttccg cagcccgaag gtgaacctgg
     5941 ctgcggcgcg tgtgcgctgt cgcttcgcga tcgaggcgtg ctctcgctgg ggtttcgtag
     6001 ttcgtttccg ctcagcatga taagcaacat gcgcgtgcgc gtgcgcgtgc cgatcgtagc
     6061 cgcctcgttc gctcttcaag gagacgagtc cgtcgacgaa agcctcacca tgctcaagga
     6121 gagcgccgtg cccaacctgt cgctcgaccc gttcaccgtc acactgggct acagcacgat
     6181 cggggccacc gcagccgagc tcgtatacgt cgtcgcggag gacgacgtct cggtctccgt
     6241 ggaagagagc gagagtgtgc tcctccaatg agctgtgact cgggacttcg tgtcgctcgc
     6301 ggcagagccg cttgcgggct aggttcggga ttgagctccg gccgccgcac gtagctcgag
     6361 ctcgagctcg agcggcagta acgtcgctgg attcagcgcg cctgcgaagc gctcaccggt
     6421 gaacgcttcg atcacggcga ttcgtcccac gatgtgcgcg ttgaattcgg cgagctccgc
     6481 tgcgggaacc cagagctcct cgtgtgttcg gccaccggcg cgctgaaccg ggtagcgcgc
     6541 cgccgtaacg tcgtcgatct cgaaccgagt cacgaagcct gcgtagccgg actgcgcatc
     6601 gacggtgttc cagtcgcgag cgatgcgccg cgcgtattcg tgggtgagca ccgggtaaaa
     6661 aatggcctgg tcggggagtc gcggcggaaa cgcgctgaac gccgactgcg cgatgagacg
     6721 gagctcgccg atgcccacgg gtcgatagag gatcatctga ttgacgcgtc gtacgctaga
     6781 tcaaattcgt acgcgtcggc acgaagctcg gcgttacgta cgctgggtcg cgcttgtacg
     6841 gggcggcgcg ggcgcggtgg gagtgctctc gagtgcgctc ggcgtctgcg ctggtgggac
     6901 cccgcgacgt cttgcgcgcc cttttcggtg aggcgcccac cccgacctga ttcgtcacgc
     6961 atgccgctac gtggagagat cgaggacgat tcgctcttca ctcgcgtcag cgagtggccc
     7021 cgtgaacgtg agcagcaacg agattcgtcc catcgagtcg atttccccgg cgtcgaacga
     7081 ttcggcgctt gtgccgtcgc ggaattcggc cacggcgcga taacgaaaaa tggtcccgca
     7141 tgcgtccact gcgctcgtca tacgaacgcg aaggaccctc ggatcggcca cgaaacgcgc
     7201 tatttcgtcg acgagagcag cgtggccgcg aatctctcgg ccgcgcgtga ccattcgacc
     7261 atcttcggcg aagcaagctc ggatcagggc cgcgcgagcg ttggggtcgc gctcaccaac
     7321 cgcgcggatg tacgtttcga cggcgatctc gactgggctc tgcatacgca ccgtatcgca
     7381 gttctcgacg tgaggcgcca gcgtcaccga caggcgtcgt acgagttttc atgttcgtcg
     7441 cgcgcgttgg attttcatgt tcgtcgcgcg cgttggaagc gatttgcaga gaactgagtt
     7501 cgatcatctc gtatcgggcg tcgcgcaacg cgcgcgatcg agagaaagcg ccgcgcattc
     7561 gcggaccaac tcggtcatgt gcgccaaagg agctcgcgaa tactcgttaa ggagtccgct
     7621 cggtgaccgc tcagatgcgc attcaacgcc gcgcggtttt cgcccgagag ccgcgccctt
     7681 ccggcatctc gagtgcggtg ccgcggtgca accacccgat ccgccgctgt cacaaggaga
     7741 tagtgaatga tcagagcgaa ccaccccacc tgggagaaac tcgtaaaggg ggaggtcaag
     7801 cacaagttcg ccgccgcatc ggcgagcatg ctgttcttca acttgcagtc tcaatacaag
     7861 cgagacccgt cgaagctgcc cgacctcgtc ggcgaggctc aaaagttttt cgagaagtac
     7921 gaagtgagtc tggaaacgga catcgcaact ctattcaagt gatcaaagga gaacccatgc
     7981 tcgctacgac ggaacaagtc gaagatctcg tctcacgagg caagagcctg atcttggcag
     8041 gcgaagagca actgctcgcg tcgctcccga aagggaattg gatcggcgga accattccct
     8101 acttcgtcgg cgcggagggc gggcgtacca cgcgcgacca tatcttcgtc caacccgtag
     8161 cggacgccag caccaagtgc acgattcaag tctacgacgc gaagacgatc tcgcaggtcg
     8221 cggtcgacag cccggagccg ggctacaccg tcttgatttt gcccgctttc agtcagattc
     8281 atcaagacta cgcgctgaac gcgccgaact acgagcagct cttcttcaag gtagtcggcg
     8341 gctggatcgc cggcattcac ctcgacgacc tcggcaagaa gtcgcccaag gtcttcaacg
     8401 gcttcaccgg cgagacgctc gcgtcggcag gcgtcgcgat gcacgtggag ctaccgccca
     8461 agcggcaagc gcagctcggt atcgtgaata tcttcgagca aggaaacggc gacgagctcc
     8521 gcttcccgaa gacgggcttc agcgcgagcg agtgcctggt caacggtgag cgccgaaacc
     8581 tcgccgagta ctacaagacg aagggcatcg actcgcgctt gccgctcgtc gccgacttct
     8641 gcggggcgat gatcaacgtg agcattcaag ccatcgacga cgcggcaggg gtcgtgaaat
     8701 tctacgcgcc ggtgttcgat acggccgtgt accgcgtcgc aaagccggtc gcgaactacg
     8761 caaaagagtt cggcggagcg atccctacca ccatcaagaa cccggtcttc tcgtgcaact
     8821 gcgtgctgaa ctatttgcac ggggagctcg agaaccgccg caccggatcc gtgaccggac
     8881 cgatgacgtt cggcgagatc ggctatcagc tgttgaacca gacgttggtc tacatcacgg
     8941 tgccttgaaa cgactgattt cgtgagggag ctccgtccga gaacgatggg ctgacgcggg
     9001 cgcgccgtca gcccatgtcg gtgagccagc tcgtgagcac gtagttcgcg accaagatcg
     9061 cgaccgcgct ctcgacgacg gcgcgcgtcg tcgcctcgcc cacgcctttg gcgccgcccg
     9121 aagcgttgaa gccctgcgca cacgaaatcg ccgagacgag aaagccgaac acgaaagctt
     9181 tgatctcacc catccagaag tccttcggaa ccaggtactt ctcgatgttg ttgatgaaca
     9241 cgcccggatc gacgccgagc cattgcacgg cgacgacgta cgcgcccacc atgccgacgc
     9301 acgtgtagag gatgcagagc agcggcaaca tcagcgttgc cgcgagcaag cgcggagaca
     9361 aaagatactg cgcggggctc acccccatcg ttacgagcgc gtcgatttgc tcggtgacgc
     9421 gcatgttgcc gagctcggcc gccatggcgc tgccggcacg ggcggtgacc atgagcgcgg
     9481 cgaagacggg gctgatctcg cgcgtgagcg agatcgcgac gagcgagccc acgatcccct
     9541 cggccccgaa gttgcgcagg ctgtgcgtcg tttggagcgc gagcaccatg ccgctgaaga
     9601 caccggtgag gccgacgatg aagatcgact gaacgccgac gaagtccatc gcctggacga
     9661 acagtgacag gcggtaaggc gggcggaaga gccaggttac gctgcgcagg gtgagatcca
     9721 gcgtcgaccc gatctgctcg agaaacttga tcaggggggc
//
LOCUS       DASTKS010000282         5317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1830223, whole genome shotgun sequence.
ACCESSION   DASTKS010000282 DASTKS010000000
VERSION     DASTKS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830223"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1592
                     /locus_tag="VFQ35_05840"
     CDS             171..1592
                     /locus_tag="VFQ35_05840"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930183.1"
                     /translation="MSKEAASRSFDELTLGSGVKLDELVDRNSLTEILSSAFDLFKIT
                     MRIFSEDGRLLADAGREIEFYSYLSGFRATRNAIEQVIGALKSKSAGTEGGVTVPCAS
                     GAEYRIHALEYDGRAVGRIIFGPFVPPGVTAPPESLLALDPSIDRARATELFGKVTRS
                     SEELVGRIAKHLSGMLGVVLFAEHRALLTSNMHLASVRESFRDLEDKNQKLQVAYDRL
                     KELDRLKSNFLATVSHELRTPLTSIIGYSEMLVEGLAGDLTNEQRDFVQTIREKGEQL
                     LALIKGLLDLSKFESGTMTLRKNNADVAKVVQEVVSTLQPGALKKDVRLEQSVEAGLP
                     RLWADPERLRQVLLNLAENAVKFTPPGGRVTVSARLGAMDAQPPGDAGGMVLLGTKRT
                     AVEFRVTDTGIGIPEAEREKVFDAFYQVDSSSTREQGGTGLGLSIVRRLVEGHDGTVR
                     IESNQPTGTVFVVTIPVRRATIG"
     gene            1623..2585
                     /gene="thiL"
                     /locus_tag="VFQ35_05845"
     CDS             1623..2585
                     /gene="thiL"
                     /locus_tag="VFQ35_05845"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET9930184.1"
                     /translation="MSAPKSSEFQRIARIQRLLEPSGGRGSALDVGIGDDAAVLRVTG
                     RVVWTVDAQVEGVHFDRRWLSLRDIGYRSFQAAASDLAAMGARATAALSSLSLPASLG
                     DDELEGIVRGQAEAARACACPIVGGNLSRGGELSLTTTVLGSAPKPLLRSEARVGDEL
                     WLVGEVGMAAAGLQALMQRARKTSAVAACIERWRRPHALLREGRILARRARAAIDVSD
                     GLAGDARHMAKASGVRLVLARAALEAALSPELVTAAPALGLSALETALSGGEDYALLC
                     SGKPDRRPPFAHAVGRVERGAGVWLEFAGRRKKLTGSFDHFATK"
     gene            complement(3119..3451)
                     /locus_tag="VFQ35_05850"
     CDS             complement(3119..3451)
                     /locus_tag="VFQ35_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930185.1"
                     /translation="MSDTQENPHGVISGEHAAMHFPISQNGVAPLQVTPHAPQLFGSL
                     SRDRQSPSHAWVPLGHAHAPLTQLVLPEQASPQAPQFFGSDDTSVQPLAHASCPGAPQ
                     EHSPSTHG"
     gene            complement(3746..3982)
                     /locus_tag="VFQ35_05855"
     CDS             complement(3746..3982)
                     /locus_tag="VFQ35_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930186.1"
                     /translation="MPQAPQLAGSLATSVQAPPHAVLPPAQPHFPPEHAWPAAHATAH
                     APQFLESLERSTQAPWQALSPEEQLELQTPLEHT"
     gene            complement(3998..4687)
                     /locus_tag="VFQ35_05860"
     CDS             complement(3998..4687)
                     /locus_tag="VFQ35_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930187.1"
                     /translation="MTQAPPHADSEPGHEQTPALQLWPLGQRTPHAPQFAVSLARLAH
                     APPHSLLPLGHRQRPALHSLPAPQLTPHAPQFRGSLETKVQAPPQSRAPEGQLQSPAT
                     QLFPPPHVNPQPPQFAGSFRMLTHRPLHATVPDGHAQAPCTHSSPLGQAFPQAPQFWS
                     SRVTSTHEPLQFWVSGAQVTAQTPFEHTSPGPHAFPHAPQLRGSLVRFVHAPEHDVLP
                     LSQIQAPLTQL"
     gene            complement(4865..5254)
                     /locus_tag="VFQ35_05865"
     CDS             complement(4865..5254)
                     /locus_tag="VFQ35_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930188.1"
                     /translation="MLQLRPEPQLFPQAPQLFESLASFTQPPPQSAEPVGHVQLPPAQ
                     TWPVPHTTPHAPQFDGSLRRSVQKPLQVRSDAGQRQTPPRQFSAGPQALPHAPQFKGS
                     LLSWTQESPHTVRFIPQLFRQAPPLQY"
BASE COUNT          819 a         1685 c         1904 g          909 t
ORIGIN      
        1 tggagcgcgc tcgacgcaga gcacgacctc gaccgaaaat tcgggttcga ctcgcaggcg
       61 ttcttggcgc aggtggccga aaagctcggc caaaagcgcg gggtgaaaga catcttgggc
      121 gcgaacgtcg gcggagccgt caacgtcgcg cgcccggagg cgaggggcgg atgagcaaag
      181 aagccgcatc tcgcagcttc gacgagctca cgctgggcag cggcgtcaag ctcgacgaac
      241 tcgtcgatcg caactcactc acggagatct tgagcagcgc gttcgatctg ttcaagatca
      301 cgatgcgcat cttctccgag gacgggcgct tgctcgccga tgcgggccgt gagatcgaat
      361 tttacagcta cctctccggc ttccgcgcga cgcgtaacgc gatcgagcag gtgatcggcg
      421 cgttgaagag caagtcggcg gggaccgaag ggggcgtcac ggtgccgtgc gcgagcggcg
      481 ccgagtaccg catccacgcg ctcgagtacg acgggcgcgc ggtgggacgc atcatcttcg
      541 gcccgttcgt gccgccgggc gttaccgctc cccccgagtc gctgctcgcg ctcgacccga
      601 gcatcgacag agcgcgcgca acggagcttt tcggcaaggt cacgcgctct tcggaggagc
      661 tagtcgggcg gatcgcgaag cacctctccg gcatgctcgg ggtcgtcttg ttcgcggagc
      721 atcgggccct cctcacgagc aacatgcacc tcgcgagcgt gcgcgaaagc tttcgagatc
      781 tcgaagacaa aaatcaaaag cttcaagtcg cttacgatcg cttgaaagag ctcgatcggc
      841 tgaagagcaa cttcctcgcg accgtctcac acgaactccg aacaccgctc acatccatca
      901 tcggctacag cgaaatgctg gtcgaggggc tcgccggtga cctcaccaac gagcagcgcg
      961 atttcgttca gaccattcgc gagaagggtg agcagctgct cgcgctcatc aagggcctgc
     1021 tcgatctctc gaagttcgag agtggcacca tgacgctccg caagaacaac gcggacgtcg
     1081 cgaaagtagt gcaagaggtc gtctcgacgc tccagccggg cgccctgaag aaggacgttc
     1141 gactcgagca atcggtggag gcgggtctgc cgcggctgtg ggccgatccc gagcgcttgc
     1201 ggcaggtgct gctgaacctc gccgagaacg cggtgaagtt cacgccgccc ggcggacgcg
     1261 tcaccgtgag cgcgcggctc ggcgcgatgg atgcgcaacc cccgggcgac gcgggcggca
     1321 tggtgctgct cggcaccaag cgcactgcgg tcgagttccg tgtgacggac accgggatcg
     1381 gcatccccga ggcggagcgc gaaaaggtgt tcgacgcctt ctaccaggtg gattcgagct
     1441 cgacgcgcga gcagggcggc accgggctcg gtctttcgat cgtgcggcgg ctcgtcgaag
     1501 ggcacgacgg caccgttcgc atcgagagca atcagccgac gggcaccgtg ttcgtggtga
     1561 cgattcccgt gcggcgtgcc accatcggct gacgcggtcg cgaacggccg tgctcggcca
     1621 tcgtgagcgc tcccaaatcc tccgagttcc agcgcatcgc gcggattcag cgcttactcg
     1681 agccgtcggg tggccgcggt tcggcgctcg acgtcggcat cggagacgac gccgccgtat
     1741 tgcgtgtgac gggccgcgtc gtctggacgg tggacgccca ggtcgaaggc gttcacttcg
     1801 accgacgctg gctgtctctg cgagacatcg gttatcgctc atttcaagcg gctgcgagcg
     1861 acctcgcggc gatgggagcg cgcgctacgg cggcgctatc gagcctctcg ctccctgcgt
     1921 cgctgggcga cgacgagctc gaaggcatcg ttcgcggcca agccgaggcg gcgcgagcgt
     1981 gcgcgtgtcc catcgtcggt ggcaatttga gccgcggcgg cgagctctcc ctgacgacga
     2041 cggtcctcgg ctctgcgcca aagccgctct tgcggagcga agcccgggtg ggtgacgagc
     2101 tctggctcgt aggcgaggtg ggaatggcgg ccgcgggtct ccaggcgctg atgcaacgag
     2161 cgcgcaagac ctcggcggtg gcggcgtgta tcgagcgctg gcggcgtccc cacgccttgc
     2221 tccgcgaggg ccgcatcttg gctcgccggg cccgcgctgc catcgacgtc tcggacggtt
     2281 tggcgggcga tgctcgtcat atggcgaagg cgagtggtgt ccggctcgtg ctcgcgcgtg
     2341 cggccctcga agccgccttg tcccccgagc tcgtcacggc tgcgcccgcg ctcggcctgt
     2401 cggccctgga aaccgccctt tcgggaggcg aggactacgc tctcctctgc agcggcaaac
     2461 ctgaccgacg gccgcccttt gcccacgcgg tggggcgcgt cgagcgtggc gcaggggtgt
     2521 ggctagagtt tgcaggccga cgaaagaaat tgacggggtc attcgatcat ttcgcgacca
     2581 aatgacgtgg ccggcagtgt gacgctgact tgttccggcc gctcggggta cttctaggag
     2641 cgcgggcgct tcgccagtcg cggatctgca gggcgagtcg ctccgagagg ccaccgatgc
     2701 gtcgactcgt ccggatcatt cgctcttggt ttcgcgcggg cccggcgttc gcgcggggtt
     2761 cggctacggc catcgtgttc gcatcgctcg cggttatgct cagcgcaagc tgcgggcgta
     2821 ctccgatcac cgagtattgc ctcgattgcg ggcaatcggg cggcgctgcg aacaccggtg
     2881 gtgcgcagaa cttgggcgga gcgaacgcgg gcacgcccgg aaacggaggt gccctgagcc
     2941 gcgggggggc cagctcgagt ggcggaaccg cctcgggcgg ggcgacctcg ggcagtggcg
     3001 gaacctcggt ggcgggggcg ggcggctcct gcgccggcgt gttgtgcggc tcgacctgca
     3061 gcgatctcaa gagcgatccg aaaaactgcg gcgtttgcgg gctaacctgc gcggccggtc
     3121 aaccgtgcgt cgacggcgaa tgctcctgtg gagcgccagg gcagctcgcg tgcgcgagtg
     3181 gctgcacgct cgtgtcgtcg gacccgaaga actgtggcgc ttgcgggctc gcttgttcag
     3241 ggagcacgag ctgcgtcaat ggcgcgtgcg catgcccgag cggcacccag gcatgcgatg
     3301 ggctctgccg atccctcgag agcgacccaa acaactgtgg ggcgtgcggc gtcacttgca
     3361 gtggcgcaac accgttctgc gaaataggga agtgcattgc ggcgtgctct ccggaaatga
     3421 cgccatgcgg gttctcttgt gtgtcgctca tcggcgaccc cctcaactgt ggcgcttgcg
     3481 gggcggtgtg cacgggcgga ctcgcgtgca tcggtgggac gtgcatctgt ccgtcgggct
     3541 tctcggagtg cggcgggcgc tgcgttgacc tgacttcgga cttcacgaac tgcggcgcgt
     3601 gcggctccgc atgtgatcgc ggcgagattt gcctggctgg caagtgcgat tgcgggagcg
     3661 ggtttctggc ctgcggcggc gcgtgcgtcg acccacagag cgacgtcaag aactgcggcg
     3721 cttgcggcaa gctctgcggt ccgggtcagg tgtgttcgag cggcgtctgc agttcgagct
     3781 gctcttcggg gctcagggct tgccagggtg cctgcgtcga cctctcgagc gattccaaaa
     3841 attgcggagc gtgcgcggtc gcgtgcgctg cgggccaagc gtgctcgggc gggaagtgcg
     3901 gttgtgcggg tggcagcact gcgtgcggcg gcgcctgcac cgatgtcgcg agcgacccgg
     3961 ccaactgcgg agcttgcggc aaggcgtgcg cctcgagtca aagctgcgtc aacggcgcct
     4021 gtatttgcga gagcggcaac acgtcgtgct cgggggcgtg cacgaatctc acgagcgatc
     4081 ctctcaattg cggcgcgtgc ggaaaagcat gcggtcccgg gctcgtgtgt tcgaacggcg
     4141 tctgcgcggt gacctgcgcc cccgacaccc agaactgcaa cggctcgtgc gtcgacgtga
     4201 cgcgcgacga ccaaaattgc ggtgcttgcg ggaaggcttg tccgagcggg ctcgagtgcg
     4261 tgcagggcgc ctgcgcatgc ccatccggca ccgtcgcgtg caacgggcgg tgcgtcagca
     4321 tcctgaacga cccggcaaac tgcggaggct gcgggttcac gtgcggcggc ggaaagagtt
     4381 gcgtcgctgg gctttgcagc tgcccctcgg gcgctctcga ctgcggcggc gcttgcacct
     4441 tcgtctccaa cgacccccgg aactgcggcg cgtgcggagt gagctgcggt gccggcaagc
     4501 tgtgcaacgc gggccgctgc ctgtgtccta gcggcagcag cgagtgcggg ggcgcgtgcg
     4561 ccaacctcgc gagcgacacc gcgaactgcg gcgcgtgcgg ggtgcgctgt ccgagcggcc
     4621 agagttgtaa cgctggcgtc tgctcgtgcc ccggctcact gtctgcgtgc ggcggggcct
     4681 gcgtcaatct cctgaacgac cagcagaatt gcggcacgtg cggtcgcgca tgcggcgccg
     4741 gtcaaacgtg catctcaggc gcctgccgct gcgatccgcg aacgaccctg tgcaacggct
     4801 cgtgcgtgga cgtccgaagc gacactcaga actgcggcgc gtgcggcgtt gcgtgtgccc
     4861 ccggtcagta ctgcagcggc ggtgcctgtc gaaacaattg cggaatgaac cgcaccgtgt
     4921 gcggcgactc ctgcgtccag ctcagaagtg atcccttgaa ctgcggcgcg tgcggcaacg
     4981 cctgcggccc tgccgagaac tgcctgggcg gcgtttgccg ttgtccggcg tcgctccgca
     5041 cctgcaacgg tttctgcacc gaccgtctca acgatccgtc gaactgcggc gcgtgcggcg
     5101 tggtgtgtgg caccggccaa gtctgcgctg gcggcagctg cacgtgcccg accggttcgg
     5161 ctgattgtgg tggtggctgc gtgaagctcg cgagcgactc gaacaactgc ggcgcttgcg
     5221 ggaacaactg cggctcgggg cgcagttgca gcaacggtca atgcctctgc gccgccgggc
     5281 aaaccgattg cggcgcgagc tgcgccgatc tcaatct
//
LOCUS       DASTKS010000283         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1830394, whole genome shotgun sequence.
ACCESSION   DASTKS010000283 DASTKS010000000
VERSION     DASTKS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830394"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..761
                     /locus_tag="VFQ35_05870"
     CDS             99..761
                     /locus_tag="VFQ35_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930189.1"
                     /translation="MPSSALERFRRSMVIGYEEWHEGTPYDLDALAELGPAERSDVEE
                     TLLSRAASDWRDVQALDVLGTPRALEALEGVLSSKEVVIRLEAAERLRRRKLLGESQF
                     EALVVESLAEATILNGMTRALGLAQKHPTPAVLKQLFACAEGGNEDIRCHAAALLHYL
                     FGFAKSSFDWDKRPFYLRFASDDPTERRAAYLELCRELGLDPNDLGELTLPRRRRRTS
                     RE"
     gene            complement(721..2775)
                     /locus_tag="VFQ35_05875"
     CDS             complement(721..2775)
                     /locus_tag="VFQ35_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9930190.1"
                     /translation="MTRVSPRFLLLLAFLAGGCASTQVGGPPIRTEAALGRVVVYRNG
                     VAYFERRATVHGNELKLDVPGERLDDFLKSLTVQDVKTGKLVPISFPTLEDQADEVTI
                     QLELPKNEVHELKITYVTESPSWKPSYRVELHGKKPARLEGWAVVHNVSGEDWKHVAV
                     GVGSTSALSFKYDLRSVHFVDRATLTDETQLGVAPPTGGSPYRVASKEVQMLGTVRRP
                     VAQPVAPPAETEHYAAGKPSAAPKAARAIEDVADSGPAGDDGLSAVAQRARGSKQKIR
                     VEGFARPADKDRKGASLANANAVRDGLVRNGVPLDQIEVVATGQTSNDVARVMAVEGE
                     AGAPTQPSSASADEPLGDAYFLTQVPLDVEKNHSAMVNVLTTDVNAEAVYFYDPLSAR
                     GSKRFAFRAIEFENPSEHTLDPGPVTVYADGQFLGEGLSDAIQPKSRAFIPFSLDKKI
                     IVETKEDGREEIDRLLTLQRGVITSEARSIRRTELEVVNRGATEATVYVRHAVAAGFK
                     LEKPTQGFEKFRDSYLIPVKVPAGASTTLRIEESTPIQKSVDIRSAAGVNELRLYLKV
                     AGGRVDADTRARLEQVIEMHRAMSELEERRQTASLQADTYRERIDELNAQLVSLRKVP
                     QAQALSRNLAQKMDEISQRLQKLTIQMADYDGQITAERVKLEDRLAELTLAKSGDAVA
                     AK"
     gene            3078..4544
                     /locus_tag="VFQ35_05880"
     CDS             3078..4544
                     /locus_tag="VFQ35_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET9930191.1"
                     /translation="MTDSLLAWPEIIQGGMGIAVSDYRLARTTSELGCLGVVSGTAID
                     TVMVRRLGLGDANGDIRRAMAKFPWPDAVSRVLRRYFVPGGKRTDQPFKLVQMPNAVM
                     SSEHLELIVIANFVEITLAKERGGRVGINFLEKIQAPLLPSLLGAILADVDAIFIGAG
                     IPFGIPDVLDRLTRWEPAELRLAVEGTPSEDTPPLRLDPAKLFGPAPFPLNRPKFFAI
                     VSSHVLAKALHKKTHGGVDGYVVEDYRAGGHNAPPRKVIDRSHPDFGPLDEPDFAQMK
                     ALGVPFWVAGCRASRTALAEAKNIGARGIQIGTVFAFCDESAIQDHLKRDVLAHARNR
                     RLRVVTDFDASPTGYPFKRIEREGLPEELTALRGRERVCDLGYLRRAVVGSDGAIGYR
                     CPGEPLDSFLAKGGDPADSANKLCLCNQLLATVGLGQTRPSGDELPLLTAGYDLTEIA
                     AVLPPGATRYSARDVLNFVVGPELVRANQARVAHTFSI"
     gene            complement(4565..>5269)
                     /locus_tag="VFQ35_05885"
     CDS             complement(4565..>5269)
                     /locus_tag="VFQ35_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HET9930192.1"
                     /translation="FGAMAYNVSHMAAGHVVNGVLLKGWPAVFALVRSYALLVGSLYF
                     ALLVLFGCVFVPIMLVTGIKIRRFMRALREPAITAFSTASSEAALPRLLEEMVSFGVP
                     RRVASFVIPTGYSFNLDGSTLYLVLASLTIAQAAKIEMSIAQQLAMVLTFMLTSKGVA
                     GVPRATLVIIAATCQSFGLPGEAGVAMILAVDELMDMARTTINVVGNGLASAVIARWE
                     GVLERPAEEPVLQPAD"
BASE COUNT          902 a         1760 c         1661 g          946 t
ORIGIN      
        1 aatcccgaac gggacgccga gtaacgctcc tatcacgctg acctcctggc gctgacgcta
       61 ccactcttgc aaaggcaaag cctatactga cgatcgtcgt gccgagttct gcgctcgaga
      121 gatttcgccg cagcatggtg attggctacg aggagtggca tgaaggcaca ccttacgacc
      181 tcgacgcgct cgccgagctc ggccccgctg aacgaagcga cgtggaggaa acgctcctct
      241 ctcgagcggc gtccgactgg cgtgacgttc aggcgctcga cgtattggga acaccgcgcg
      301 cgctcgaggc gctcgagggc gtgttatcgt cgaaagaggt cgtcatccga ctcgaagccg
      361 ccgaacgatt acgaaggcgt aaactcctcg gcgagtcgca gttcgaagcc ctggtcgtcg
      421 aatcgctcgc cgaagcgacc atcctgaacg gcatgactcg cgcgctcggc cttgcccaaa
      481 agcaccccac ccccgcggtg ttgaagcagc tcttcgcgtg cgccgagggc ggcaacgaag
      541 acattcgctg ccacgccgcg gcgctcctgc actacctgtt cgggttcgcc aaatcgtcgt
      601 tcgattggga caagcggccc ttctatttgc gcttcgcctc ggacgatccc acagagcgac
      661 gcgcggcgta cctagagctc tgccgcgaac tcggcttgga cccgaacgac ctcggggagc
      721 tcactttgcc gcgacggcgt cgccggactt cgcgagagtg agctcggcca agcggtcttc
      781 gagctttacg cgctcggccg tgatctggcc atcgtagtcc gccatctgga tcgtgagctt
      841 ttgcaggcgc tggctgatct cgtccatctt ctgcgccagg tttcggctca aggcttgcgc
      901 ttgcggcact ttgcggagcg acacgagctg cgcgttgagc tcgtcgatgc gctcacggta
      961 cgtgtctgcc tgcagcgacg cagtttgacg gcgctcctcg agctcgctca tggcgcgatg
     1021 catttcgatg acctgctcga ggcgggcgcg cgtgtcggcg tccacgcggc cgcccgcgac
     1081 tttcaagtag agcctgagct cgttgacgcc ggccgcgcta cggatgtcga ccgacttctg
     1141 aatcggcgtc gactcctcga tgcgcagcgt cgtcgaagcg cccgcgggaa ccttcacggg
     1201 aatcaagtag ctgtcgcgga acttctcgaa cccttgcgtc ggcttttcga gcttgaaacc
     1261 cgcggcgacc gcatgccgca cgtacacggt ggcttcggtc gcgccacggt tcacgacctc
     1321 gagctccgtc ctgcggatgc tgcgcgcttc gctcgtgatg acgccccgct gcaaggtgag
     1381 cagccgatca atctcttcgc ggccatcttc tttggtctcg acgatgatct tcttgtcgag
     1441 cgaaaacggg atgaacgcgc ggctcttcgg ctgaatcgcg tcgctcagcc cctcccccaa
     1501 gaactgccca tccgcgtaga cggtgaccgg ccccgggtcg agcgtgtgct cgctcgggtt
     1561 ctcgaactcg atggcgcgga acgcaaagcg cttcgagccg cgcgccgaaa gcggatcgta
     1621 aaagtacacc gcctccgcgt tcacgtcggt cgtcagcacg ttcaccatcg cgctatggtt
     1681 cttctccacg tcgagcggca cctgcgtcag aaagtaagcg tcgcccagcg gttcgtcggc
     1741 gctggccgag ctcggctggg tcggggcacc cgcttcgcct tccaccgcca tcacccgggc
     1801 gacatcgttc gaggtctgcc cggtcgccac cacctcgatt tgatcgagcg gcacgccgtt
     1861 gcgaaccaag ccgtcacgca ccgcgttcgc attcgccagc gacgcccctt tgcgatcttt
     1921 gtctgcgggg cgcgcaaacc cctcgacgcg aatcttttgc ttcgagccgc gtgcgcgctg
     1981 cgccacggcg ctcaggccgt cgtcgccagc cggacccgag tcggcgacgt cttcgatggc
     2041 gcgcgctgct ttcggagccg ccgaaggctt gccagcggcg tagtgctcgg tttcggcagg
     2101 cggagccacc ggttgcgcca cggggcggcg aaccgtgccg agcatctgca cttctttact
     2161 cgcgacgcga tacggtgagc cgcccgtcgg tggcgccacg ccgagctgcg tctcgtccgt
     2221 cagcgtcgct cggtccacga agtgcacgct gcgaaggtcg tacttgaacg acagcgcaga
     2281 ggtcgagccc acgccgacgg cgacgtgctt ccagtcttcg cccgagacat tgtgaacgac
     2341 ggcccagcct tcgaggcgcg ccggcttctt gccgtggagc tccacgcggt agctcggctt
     2401 ccacgatggg ctctcggtta cgtacgtgat cttgagctcg tgcacctcgt tcttggggag
     2461 ctcgagctgg atcgtcacct cgtccgcttg atcttccagg gtggggaaag agatcggcac
     2521 gagcttaccg gttttgacgt cttgcaccgt gagcgacttc aagaagtcgt cgaggcgctc
     2581 accgggcacg tcgagcttga gctcgttccc gtgcacggtc gcgcgccgct cgaagtacgc
     2641 aacgccgttg cgatagacga ccacgcggcc aagcgcggcc tcggtgcgaa tcggcgggcc
     2701 acccacctgc gtcgacgcgc aacctccagc caagaaagcc aagagaagaa gaaacctggg
     2761 gctaactcgc gtcatggggg ctccgggagg ccgaatcggc cgacaaggga cggttacgtt
     2821 tacgctcgga gcggcggttg catccccgag caccctgcac aacgcagctc tcctcgaaac
     2881 cttacgctcg ccggcggtgc tcgcggtcgc acaacgtcgc accatgggtg caccgaaaac
     2941 gcggcctctg ggcgcccgag cagacagcgt gcggctcgac tgatggtcgt catctcgacc
     3001 aacatcgctc cgtcacatgt cggtcacgcg ccgcccgcgg tgctgcattg gatcgcgccg
     3061 gcactaagct gcgcgccatg actgactctc tccttgcttg gccggagatc atccaaggcg
     3121 gaatggggat cgcggtgtcg gattaccggc tcgcgcgcac cacctcagag cttggatgcc
     3181 tgggggtagt ttcgggcact gcgatcgaca ccgtgatggt gcgccggttg gggctcggcg
     3241 acgcgaacgg agacatcaga cgagcgatgg ccaagtttcc ttggcccgac gcggtctcac
     3301 gcgtactgcg gcgctacttc gttcccgggg gaaagcggac ggatcagccg ttcaagctcg
     3361 ttcagatgcc gaacgcggtg atgtcctcgg agcacctgga gctcatcgtc atcgcgaatt
     3421 tcgtggagat aacgctagcg aaagagcgcg gcggacgggt cggcatcaac tttctggaaa
     3481 agatccaggc gccgcttctt ccttcgctcc tcggcgccat actcgcagac gtcgacgcga
     3541 tcttcatcgg cgctgggatc ccattcggca ttcccgacgt gctcgatcgg ctgacccgct
     3601 gggagccagc cgagctccgc ctcgctgtcg agggaactcc gagcgaggac acgccgccgc
     3661 ttcggctcga tcccgcgaag ttgttcggcc ccgcgccatt tccactgaac cgccccaagt
     3721 tcttcgcgat cgtctcctcg cacgtgctcg ccaaagcact gcacaagaag acgcacggtg
     3781 gtgtcgacgg ttacgtggtc gaagactacc gcgccggcgg gcacaacgcg ccgcctcgta
     3841 aggtgatcga tcgatcgcat ccagacttcg gacctctgga cgagcccgat tttgcgcaga
     3901 tgaaggcgct cggtgtgccc ttctgggtag cgggctgccg agcgtcgcgc acggcgctag
     3961 cggaagcgaa gaacatcggc gcgcgcggga ttcagattgg aaccgtgttc gcgttttgcg
     4021 acgagtcagc catccaggac cacctcaagc gcgatgtgct cgcacacgcc cgcaatcgcc
     4081 gactgcgtgt cgtcaccgat ttcgacgcct cgccgacggg ctatccgttc aagcgcatcg
     4141 agcgcgaggg gctgcccgaa gagctcactg cgctgcgcgg ccgcgagcgc gtgtgcgacc
     4201 tcggctactt gcggcgagct gtcgtcggga gcgacggagc gattggatat cggtgcccgg
     4261 gagagccgct cgactcattc ctcgccaagg gtggtgaccc ggccgactca gccaacaagc
     4321 tttgcttgtg caatcagctg ctagcgacgg ttggactcgg acagacgcgc ccatcgggcg
     4381 acgaactgcc gttgctgaca gctggctacg acttgaccga gatcgcggca gtgcttccgc
     4441 cgggcgccac acgctactcg gcgcgcgacg tgctgaactt cgtggtggga ccggagctcg
     4501 ttcgtgcgaa tcaggcgcga gtcgcgcaca ccttctcgat ttgacgcggg cgagggcagc
     4561 gtcctcaatc cgccggttgc agcaccggct cttcggcagg gcgttccaag acgccctccc
     4621 agcgagcgat gacggccgac gcaagtccgt tccccaccac gttgatggtg gtgcgggcca
     4681 tgtccatgag ctcgtcgacc gccaagatca tcgcgacgcc ggcttcgccc ggcagcccga
     4741 agctttggca cgtcgccgcg atgatcacga gcgttgcgcg cggcacgccc gcgacgccct
     4801 tcgacgtgag catgaaggtg agcaccatcg cgagctgctg cgcgatgctc atctcgatct
     4861 tggccgcctg cgcgatcgtc agcgacgcga gcaccagata caaagtcgac ccgtcgaggt
     4921 tgaacgaata gcccgtcggg atgacgaagc ttgcgactcg gcgcggcacg ccgaagctga
     4981 ccatctcttc gagaagccga ggcaacgccg cctcgctcga cgcggtagag aacgccgtga
     5041 tggctggttc gcgcagcgcc cgcatgaagc gccgaatctt gatgcccgtc accagcatga
     5101 tcggaacgaa gacgcaaccg aacagcacga gcagagcgaa gtagaggctc cccacgagca
     5161 gggcgtagct cctcacgagc gcgaagacgg cgggccagcc tttcagcagc acgccgttca
     5221 cgacgtgacc cgcggccatg tgcgacacgt tgtacgccat cgccccgaa
//
LOCUS       DASTKS010000284         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1384294, whole genome shotgun sequence.
ACCESSION   DASTKS010000284 DASTKS010000000
VERSION     DASTKS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384294"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VFQ35_05890"
     CDS             <1..1425
                     /locus_tag="VFQ35_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9930193.1"
                     /translation="VGDDYFVATSTFEYFPGVPIYQSRDLVEWRLIGHALTRDEQLSL
                     QTAKSSQGIFAPTLRYHAGIFYLVTTDVSGGGNFYVTATHPAGPWSNPICLEKEACWM
                     DPSLFFDDDGRVYFTRHGGGRHGAIYQAELDLARGKLVSEPRLIWSGTGDIWPEGPHL
                     FKRGGWYYLTNAEGGTSYEHMQVIARSRSPYGPFEGYAKNPILTHRTRPEHPVQALGH
                     ADFVEGPDGRWWMVFLGVRPSAERHHHLGRETFLAAVEWDAEGWPSVKLEETARAPSN
                     RVVRDDFDAQQLALEYNFVRNPERARYRLDARAGFLRLLGSPTGLDEVGAPTFVGRRQ
                     QHFDCRIRAALEFEPAQSSDEAGLVLRANEDNHFALLVQSAGAGRELVLRARRRGATE
                     IVARAELSPGPLVLEVRATRERYEFACRAPAPIVLGSLPTRDLSTETTGGFTGVYAGM
                     YAKSPSAEPPGADFDWFEYAPGDA"
     gene            1585..4707
                     /gene="secA"
                     /locus_tag="VFQ35_05895"
     CDS             1585..4707
                     /gene="secA"
                     /locus_tag="VFQ35_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET9930194.1"
                     /translation="MLTWVAKKLFGTSNDRAVKRMQPLVVRINQLEDSLKKLSDAELQ
                     AKTPEFKQKLENGASLDDILVEAFAVCREASRRVLRMRHYDVQLMGGMVLHRGSIAEM
                     RTGEGKTLVATTAVYLNALEGKGVHLITVNDYLAKRDAEWMGRLYTWLGLSIGTIVNG
                     QTDAEKKRSYQSDICYGQNNEFGFDYLRDNMKFSSLDYAQRPLNFAIVDEVDSILIDE
                     ARTPLIISGTAEAASEKYRTLNEVVAQLRKDEHYNTDEKGFSATLTDEGVEFVQRAVG
                     VDNLYDPVNIQSLHILNQLLRAHALYKRDQHYMVAPDGKVLIIDEFTGRVLPGRRWSD
                     GLHQAVEAKENVRIQEESRTMATITFQNLFRIYKKLAGMTGTAETEAQEFSSTYKLDV
                     VAIPTNKAMIRVDDHDVVYKTEREKFNALVRDIITHHEAGRPVLVGTTSVEKSAAIAR
                     ILKKKGIVHNVLNAKHHENEAFIVAQAGKRGAVTVSTNMAGRGTDIMLGGNAEMLAKA
                     ELKEKGMAPESHPEELKALIARHEEECKAEHDEVIGIGGLYIIGTERHESRRIDNQLR
                     GRAGRQGDPGYSKFYLSLEDDLMRIFAGDRVKNLMDRMGMPDDEPIEHPLVTRSIENA
                     QRKVEERNFDVRKNLLEYDDVMNAQRKTVYALRQQLLLGRYTPEEVDDEGKPLGTVRE
                     ITVDPKIAELVSREVGMMVGLFCDPQLQPIGDDKKPRAPTRDEIAKTGDIVELEQLRH
                     EVYDYWGVKMDFPEGKPQKAVDVYDELQKLVPLGLTEQRERVLDLIDRVVGAIVEESC
                     PPNKQPEDWDWPGMFQGFEEHFKERPDQKIDELGDAELVVRRLYEAAQEIYKKKEAEL
                     GLETALRVFRHMYIESIDEIWVDHLSNMEHLRDGIGLRGYGQRDPKNEYKKESYDLFL
                     TMMAKISSNVIVRLFDARPRRREELAAAEAEAARRYQQELEQTVAQHPTAESEDPTAL
                     LAELERVVQTATAQAPAPAPKRREAPKIGRNDLCPCGSGKKFKKCHGALLDDGSGGDD
                     DDDGEQQPQV"
BASE COUNT          955 a         1598 c         1574 g          740 t
ORIGIN      
        1 gtaggcgacg actacttcgt cgcgacgagc acgttcgaat atttccccgg cgtgccgatt
       61 tatcagagcc gcgatctcgt cgagtggcgg ctgattgggc acgctttgac ccgcgacgag
      121 cagctttcgc tccaaacggc gaagagctca caaggcatct tcgcgcccac gctgcgctac
      181 cacgcaggaa tcttttacct cgtcactacc gatgtctctg gcggtggcaa tttctacgtg
      241 accgcgacgc atccggccgg tccgtggtcg aacccgatct gtctcgagaa ggaggcgtgc
      301 tggatggatc cgtcgctctt cttcgacgac gacggtcgcg tctacttcac tcgccacgga
      361 ggcggccgac atggtgcgat ttatcaggcc gagctcgacc tcgcgcgcgg caagctcgtc
      421 tccgaaccgc gcctgatttg gtcgggcacg ggcgacatct ggcccgaagg tccgcacctc
      481 ttcaaacgcg gtggttggta ctacctgacg aacgcggagg gcggcacgag ctacgagcac
      541 atgcaggtca tcgcgcgctc gcgctctccg tacggtccct tcgagggcta cgccaagaac
      601 ccaatcttga cgcaccgtac acggcccgaa catccggttc aggcgctcgg tcacgccgac
      661 ttcgtggaag ggccggacgg gcgctggtgg atggtttttt tgggggttcg cccgagcgcg
      721 gagcgacacc accatttggg ccgcgagacc tttctcgccg cggtggagtg ggacgcagag
      781 ggctggccga gcgtgaaatt ggaggagacc gcgcgcgctc cttcgaatcg cgtggtgcgg
      841 gacgacttcg acgcccagca actggcgctc gagtacaact tcgtgcggaa ccccgagcgt
      901 gcgcgctacc gtctcgacgc gcgcgctggt ttcttgcggc tgctcgggag ccccacgggc
      961 ctcgacgagg tcggcgctcc cacgttcgtc gggcgccggc aacagcactt cgactgtcgc
     1021 atccgcgcgg cgctcgaatt cgagcccgcg cagtccagtg acgaagcggg gctcgtgctc
     1081 agggcgaacg aggacaatca cttcgcgcta ctcgtacagt cggccggggc ggggcgagag
     1141 ctcgtgttgc gggctcggcg tcggggcgcg acggagatcg tcgcccgggc ggagctttcg
     1201 cccggccccc tggtgctgga ggtgcgcgcg actcgggagc gttacgaatt tgcgtgcagg
     1261 gcgcccgcgc ccatcgtgct cggtagcttg cctactcgcg acttgtcgac cgaaacgact
     1321 ggcgggttca cgggcgtgta cgcgggcatg tacgcgaaga gcccgagcgc cgagccaccc
     1381 ggcgcggatt tcgattggtt cgagtacgct ccgggcgacg cctagcggca aaaatcgcct
     1441 acctgcctgc tcgcagagtg gcacggccga gcgtgacgga gtagcttgcc ggtgcttcgc
     1501 cggccaggag gcccaaggcg tggtcgagag accgcccccc aagccatttg ctcccgacga
     1561 agaggaagct gaatcgcgct cgccatgctg acctgggtcg ccaaaaaact gttcggtacc
     1621 tccaacgatc gcgccgtcaa gcggatgcag ccgctcgtcg tgcgcatcaa ccagctggaa
     1681 gactcgctga agaagctgtc cgacgcggag cttcaggcga agacgcccga gttcaagcaa
     1741 aagctcgaga acggcgcctc cctggacgac attttggtcg aggcgttcgc ggtgtgccgc
     1801 gaggcgagcc gccgcgtcct ccgcatgcgc cactacgacg tgcagctgat gggtggcatg
     1861 gtgctgcacc gtggctcgat cgccgagatg cgcaccggcg aaggcaagac gctcgtcgcc
     1921 accaccgccg tttatctcaa cgcgctcgaa ggcaagggcg tgcacctcat cacggtgaac
     1981 gactacctcg ccaagcgcga cgcggagtgg atggggcgcc tctatacctg gctcggcctc
     2041 agcatcggta cgatcgtcaa cggccagacc gacgccgaga agaagcgctc gtatcagtcg
     2101 gacatctgct acggccagaa caacgagttc ggcttcgact acctgcgcga caacatgaag
     2161 ttctcgtcgc tcgactacgc gcagcgcccg ctgaacttcg ccatcgtcga cgaggtcgac
     2221 tccattctca tcgacgaggc gcgcacgccg ctcatcatca gcggcacggc cgaggccgcg
     2281 agcgagaagt accgcacctt gaacgaggtg gtcgctcagc tccgtaagga cgagcactac
     2341 aacaccgacg aaaagggctt cagcgccacg ttgacggacg aaggtgtgga gttcgtgcag
     2401 cgcgccgtcg gcgtcgacaa cctgtacgac cccgtcaaca tccagtcgct ccacatcttg
     2461 aatcagctgc tccgcgcgca cgccctctac aagcgcgatc agcactacat ggtggcgccc
     2521 gacggcaagg tgctcatcat cgacgagttc accgggcgcg tgctccccgg tcgtcgctgg
     2581 agcgatggct tgcaccaggc ggtcgaggcc aaggagaacg tgcggatcca ggaagagagt
     2641 cgcacgatgg cgacgatcac cttccagaac ctgttccgca tctacaagaa gctcgccggc
     2701 atgaccggca ccgccgagac cgaggcgcag gagttctcgt cgacgtacaa gctcgacgtg
     2761 gtggcgatcc ccaccaacaa ggcgatgatc cgcgtcgacg accacgacgt cgtctacaag
     2821 accgagcgcg agaagttcaa cgcgctcgtc cgcgacatca tcacgcacca cgaggcgggc
     2881 cgccccgtgt tggtcggcac gaccagcgtc gagaagagcg ccgcgatcgc ccgcatcttg
     2941 aagaagaagg gcatcgtcca caacgtgctg aacgcgaagc accacgagaa cgaagcgttc
     3001 atcgtggcgc aagcgggcaa gcgcggcgcg gtcaccgtct ctacgaacat ggccggtcgt
     3061 ggcaccgaca tcatgttggg cggcaacgcc gagatgctcg cgaaggcgga gctgaaagag
     3121 aaggggatgg ctcccgagag tcaccccgag gagctcaagg ctctcatcgc gcgccacgaa
     3181 gaagagtgca aagccgagca cgacgaggtg atcggcatcg gcggcctcta catcatcggc
     3241 accgagcgcc acgagtcgcg ccgcatcgac aaccagctgc gtggccgcgc cgggcgtcag
     3301 ggcgaccccg gctacagtaa gttctacctg tcgctcgaag acgacctgat gcgcatcttc
     3361 gccggcgacc gcgtgaagaa cttgatggat cgcatgggca tgcccgacga cgagcccatc
     3421 gagcaccccc tcgtcacgcg cagcatcgag aacgcgcagc gcaaggtcga ggagcgcaac
     3481 ttcgacgtcc gcaagaacct gctcgagtac gacgacgtga tgaacgcgca gcgcaagacg
     3541 gtgtacgcgc tgcgccaaca gctcttgctc ggccgttaca cccccgaaga ggtcgacgac
     3601 gagggcaagc ccctcgggac ggtgcgcgag atcacggtcg acccgaagat cgccgagctc
     3661 gtgagccgcg aagtgggcat gatggtgggc ttgttctgcg acccgcagct ccagccgatc
     3721 ggcgacgaca agaagccccg cgcgccgacg cgcgacgaga tcgcgaagac cggcgacatc
     3781 gtggagctcg agcagcttcg ccacgaggtg tacgactact ggggcgtgaa gatggacttc
     3841 ccggagggga agccgcagaa ggccgtcgac gtctacgacg agctacagaa gctcgttccg
     3901 ctcggcctca ccgagcagcg cgaacgcgtc ctcgacctca tcgatcgcgt cgtcggcgcc
     3961 atcgtcgagg agagctgccc tcccaacaag cagccggaag actgggattg gccgggcatg
     4021 ttccagggct tcgaggagca cttcaaggag cgccccgacc agaagatcga cgagctcggc
     4081 gacgcggagc tcgtggtgcg tcggctctac gaggcggccc aagaaatcta caagaagaag
     4141 gaagcagagc tcgggctcga gaccgcgctg cgcgtcttcc gccacatgta catcgagagc
     4201 atcgacgaga tttgggtcga ccacctctcg aacatggagc acctgcgcga cggcatcggc
     4261 ctgcgcggct acggccagcg cgaccccaag aacgagtaca agaaggagtc ttacgacctc
     4321 ttcttgacga tgatggcgaa gatctcgagc aacgtcatcg tgcgcttgtt cgacgctcgc
     4381 ccccgtcgtc gcgaggagct cgccgcggcc gaagccgaag ccgcgcgccg ctatcagcag
     4441 gagctcgaac agacggtcgc gcagcacccg accgccgaga gcgaggatcc gacggcgctg
     4501 ctcgccgagc tcgagcgcgt cgttcaaacc gccacggctc aggctccggc gcctgcgccc
     4561 aagcgccgcg aagccccgaa gatcggtcgc aacgacctct gcccctgcgg ttcgggcaag
     4621 aagttcaaga agtgccacgg cgcgctgctc gacgacggca gtggcggcga cgacgacgac
     4681 gacggcgagc aacagccgca ggtctgacgg ctaaccgggg gcgcgccgac tcgaagcgcg
     4741 ccccgccgtc tacacgcacc acaagtcgtt cagacgaagc gagattgcgt cgaagggctc
     4801 gcagcgcacc tcggcgtcac cgcgccagac tccgaggatg cgatatccat tgccgtccag
     4861 gaatagc
//
LOCUS       DASTKS010000285         9742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1608287, whole genome shotgun sequence.
ACCESSION   DASTKS010000285 DASTKS010000000
VERSION     DASTKS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9742
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608287"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VFQ35_05900"
     CDS             complement(<1..585)
                     /locus_tag="VFQ35_05900"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930195.1"
                     /translation="MLLIRKPMGVRRRVVSGGGGETTVGNLLQTLTEGNSAGNKQIRD
                     LLAGTIPGDAVNLGQLSALSNYVDSQIAALDGQIDTVGAGAVRTLAQILAADPSAGGA
                     KITELADGTAPADAATRGQLDASASSITTAYTAAIAARTLAQVLAAGNAANAKITAVT
                     DGTAATDAVNRGQLDASASSITTAYTAAIAARTLA"
     gene            complement(588..956)
                     /locus_tag="VFQ35_05905"
     CDS             complement(588..956)
                     /locus_tag="VFQ35_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930196.1"
                     /translation="MNSFGMLARGLSVPAPQKSTYGGAANFARYSIGPSTIAISLGTL
                     RGSESGSFVVKNTGEQTIYLLCSDGSPGEVVLSADGTEGTQLGWPLMAGEVETFTDVT
                     VGTLFAWCATASDLWIRVGA"
     gene            complement(953..1768)
                     /locus_tag="VFQ35_05910"
     CDS             complement(953..1768)
                     /locus_tag="VFQ35_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930197.1"
                     /translation="MNERFGGGGQDIERQRGYAREVHREQALAPTTRARPKMGGQSPL
                     PSVVTAAENYPNGGRVTPVDLFSLQLPDLEIWERAQPGWVIRLTSPARPPGYILDIWT
                     LQPLFLELGVISGGFEQRMIVDVAPAFSLQVACERIWGRLGWSSTEVKPPPGALLDWQ
                     VTRGICETTARRAFLLSSIEGSHEVPGFAGSFSLITGNQYPSNLRNLSGGLALSLVFA
                     GDDGELIQTFDRDQILGLLNDGQRVPLPPFARTCGWTGSNIPLRIVYHFGGNR"
     gene            complement(1765..2643)
                     /locus_tag="VFQ35_05915"
     CDS             complement(1765..2643)
                     /locus_tag="VFQ35_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9930198.1"
                     /translation="MEDPSPTQSTNSNRTRNGSGKPDGWDTWLTAVQVARRLRCHRQH
                     VYRLALNGDLTFYDGMFRGRLQRRFEPGSVEAYAQSRPQNEQSASSSSDDDSDSGIPG
                     AEYFEESDLEREGGGARDGALVELSRLMARGLTDLREVATDARKGQHEAYSLIAVPSR
                     EMFKLALDQLRAAYKRIRELEERLNRLHDEQRDARRDDREFSLVEKQLNASAEQKDQF
                     FKMLLSNVPTVFAQLKDTIERRTTGPLARWTASLSPEKQTRLVGAIEAVMSVEDELKS
                     AASPSASSDTEGQQAP"
     gene            complement(3058..3912)
                     /locus_tag="VFQ35_05920"
     CDS             complement(3058..3912)
                     /locus_tag="VFQ35_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9930199.1"
                     /translation="MASISDREAKKIVDSLVDELGLSPVESLAVRLVGKHESGYGMGW
                     KRPGSVGSNNWGAITRPTGGPDTFEHEDSKWNPKTGKVEQYVTHFQKYATPRDGARGL
                     ALQLLFEGANPKKPRRANVAAALERRSIPDLAAAMRFNRYFLGVKPPEQAIADYAAAL
                     ERMYQDIRADTGEDLFDRPLVRNASQTEHSELRPEGSESSDSSSPRASLERLCRSLPE
                     LRRGVRGDVVAILQFEIGCDPDEIFGPLTERALRAWQAERGLEPSGVVGPETWATIYG
                     LASREQQA"
     gene            complement(3925..4689)
                     /locus_tag="VFQ35_05925"
     CDS             complement(3925..4689)
                     /locus_tag="VFQ35_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930200.1"
                     /translation="MDGWEIGIDETAIGVGPDEIGFSFGKLVKGIGRGVSSAAKATAN
                     VSRAIVQSPITKTAVGVVAVAFPAVGVPAAAAVATANLAIDRVQAGKVAISTVNANIS
                     KLKRQAEGGNRSAAHAVNAMQVAIAQRKARKVGKPAPRPVAAPARHVARVPAAAAQVP
                     TSRYAPSPKPAQLALTLAAGGSALGKRTLNAVNSGQSVELPSGAVVVPGKKPIKGRRV
                     WVGRAPAGVRASLVQGGHAVTKSGYVITGQRIYVAT"
     gene            complement(4741..5220)
                     /locus_tag="VFQ35_05930"
     CDS             complement(4741..5220)
                     /locus_tag="VFQ35_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930201.1"
                     /translation="MGPIFFIGALAIGALLLRPTKTKVKTLDEGLIDGSEAQQLANTE
                     RLAAARFQIQSLIAKNSISFARQSADYIEKTLGMPRTANNVRAYATMLDENRAAGGTA
                     VAGEIGWEPAPPDWLKFHATQSKLGGDPAQIRATAETMRRYGYRRAARIHLRLLGES"
     gene            complement(5228..6001)
                     /locus_tag="VFQ35_05935"
     CDS             complement(5228..6001)
                     /locus_tag="VFQ35_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930202.1"
                     /translation="MIPVLPLALGAGLVALMFTGGKKGASSSSSSPGGGTAPPAGGGF
                     VPSTTTPAGGKTAIDFQARILDALSRNSPDALMQIANEMDAAGLKTEAAALRVTASAL
                     RTVGTVAPSTAVPNPPIVTPPQIVLPPVVTPPVGPSPVPLPPGNPITSQQAQRLALAQ
                     QVAMHLQNTSRYKEDQALVKAFQSQEGLVVDGLYGYKSALALVSYGIVPPRPFYWRSS
                     SAKADKSAYSKALIAQSVADPARAADWRAASKVENDPNK"
     gene            complement(5998..6513)
                     /locus_tag="VFQ35_05940"
     CDS             complement(5998..6513)
                     /locus_tag="VFQ35_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930203.1"
                     /translation="MARGFTIRLGALLGDDPRDYAEFVRSGLRSLIIANVWAYRHDPT
                     LPSVYDAGCVYRREAPGNEQFCDVRTVLERGFGDCEDLACGVASWRIVRTGELARPQI
                     TWTRLPTGRYLYHITVLRADGTTEDPSRELGMGNEPGEYVPRGAFYVYQLFPGRSGLV
                     PPPVPIERVSA"
     gene            complement(6513..6980)
                     /locus_tag="VFQ35_05945"
     CDS             complement(6513..6980)
                     /locus_tag="VFQ35_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930204.1"
                     /translation="MGLYDDETGATDIYLDSGTVSGENDPETGAARARQRQQPAKGTP
                     AIRAKAFLPSVAIGAGATAPLDMKLNFAFKAIGMRLSGQNQDKLLYNGATIRGRPQEA
                     NSGSTGCEIFTHAGETFFEEWDTANPNEQFSLSFTNTHTASVTPTGYLIGYRE"
     gene            complement(7146..7529)
                     /locus_tag="VFQ35_05950"
     CDS             complement(7146..7529)
                     /locus_tag="VFQ35_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930205.1"
                     /translation="MTPTQKLEAAESRLESYRRKLKAMQGAGELATERLTGCVMTVAG
                     GAFAGVVWSKFGDGTPGSAKFGDTSFEIDTMIGFACVGAAAFGMAGKHSAELGTFGGG
                     MLAARVAALVGRVLEEHEKKNAAKN"
     gene            complement(7573..8136)
                     /locus_tag="VFQ35_05955"
     CDS             complement(7573..8136)
                     /locus_tag="VFQ35_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930206.1"
                     /translation="MTIERRNPLPRAVYWQDVFTPAIGEHGGTIEEFRAWASAHKNAV
                     LIRQTVYHGPGADPFAELIANSGWVINPLLAKLAADAAAAPDFGRSRLWALFEVREPV
                     PWDAIKFGYPTIAERGTETTEGDTVTKPPPEPDAIDRVDDAIDSAIDSAKDVAKTTGS
                     IVGVLALAAGALLFFNLLAASRTARAR"
     gene            complement(8133..8489)
                     /locus_tag="VFQ35_05960"
     CDS             complement(8133..8489)
                     /locus_tag="VFQ35_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930207.1"
                     /translation="MLRRTLLDHPSNDQILEGARVTLEKWFAREQPWGAQIIANVRGV
                     VRRRAIELPDYCSVAATSDGLRPRLTMAGDTFASVALELLYFGGADAMPWPVLDTRGE
                     WLPHDCVWALDLVGAA"
     gene            complement(8987..9175)
                     /locus_tag="VFQ35_05965"
     CDS             complement(8987..9175)
                     /locus_tag="VFQ35_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930208.1"
                     /translation="MREMSPRPPLASEQKRIERAKQRLCAASCAALRLTLEPCALCPA
                     CDAGERALLFFARLGGKI"
     gene            9430..>9742
                     /locus_tag="VFQ35_05970"
     CDS             9430..>9742
                     /locus_tag="VFQ35_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930209.1"
                     /translation="MRKFGLWAFAFVLLLACSEEHPPDAPSSMPTGGRASGGSSSAGA
                     SEQPSDGGSVAEPESGGRTATTGGRRASGGASSAGSVGSGGRAPTGGTTAEGGASESE
                     GG"
BASE COUNT         1577 a         3304 c         3044 g         1817 t
ORIGIN      
        1 cgccagcgtc cgagccgcaa tagcagccgt gtaggccgtc gtgatcgagc tcgcgctcgc
       61 gtccagctgt ccgcggttca cggcatccgt tgccgccgtg ccgtctgtga ccgccgttat
      121 tttcgcgttc gcggcgttcc ccgctgcgag cacttgcgcc agcgtccgag ccgcaatcgc
      181 ggctgtgtag gccgtcgtga tcgagctcgc gctcgcgtcc agctgaccgc gcgtcgcagc
      241 gtccgccggc gccgtgccgt ctgctagctc cgtgattttg gcgccgcccg ccgatggatc
      301 cgccgcgaga atctgcgcca gcgtgcgcac tgcgcccgcg cccaccgtgt cgatctgacc
      361 gtctagcgct gcgatctgcg agtcgacata gttggagagg gcggaaagct ggccgaggtt
      421 caccgcgtcg cccgggattg ttcccgccag caaatctcgg atctgcttgt ttccagcgct
      481 gttaccttcg gtcaacgtct gcagtaggtt gcccacggtc gtttcacctc cgccgccgct
      541 caccactcgg cggcgcactc ccatcggttt gcgaatcagc agcatggtta ggctcccacg
      601 cggatccaga gatcggacgc agtcgcgcac caggcaaaca gcgtcccgac agttacatcc
      661 gtgaacgtct cgacctcgcc agccattagc ggccagccga gctgtgtgcc ctccgtgccg
      721 tccgccgata gcaccacctc gccgggtgag ccgtccgagc agagcagata gatcgtctgt
      781 tcaccggtgt ttttgactac gaatgatccg gattcactgc cccggagtgt gccgagcgag
      841 atcgcgatcg tgctcggacc aatcgagtaa cgagcgaagt tcgcggcgcc gccataggtc
      901 gacttttgcg gcgcgggaac actgagccct cgcgcgagca tgccgaaaga gttcatcggt
      961 tccccccgaa gtggtagacg attcgcagcg ggatattcga gccggtccag ccgcacgtgc
     1021 gcgcgaacgg cggcagcggc acgcgctgac cgtcattcag caggccgagg atttgatcgc
     1081 gatcgaacgt ctgaatcagc tccccatcgt cgccagcgaa caccagcgag agcgcgaggc
     1141 cgccgcttag gttgcgcagg ttggacgggt actgattacc cgtgatcagc gaaaagctgc
     1201 ccgcaaatcc gggcacctcg tgagagccct cgattgagct cagcaggaac gcgcgccgcg
     1261 cggtcgtctc gcagatgccg cgcgtcacct gccaatcgag cagagcgccc ggcgggggct
     1321 tgacctcggt gctcgaccag ccgagccggc cccaaatgcg ttcacacgcc acctgaagac
     1381 taaacgccgg tgcgacatca acgatcatgc gttgctcgaa gccgccgcta atgacgccga
     1441 gctccaaaaa cagcggctgc aacgtccaaa tgtcgaggat gtagcccggc ggccgcgctg
     1501 gactcgtcaa ccgaatcacc cagccgggct gcgctcgctc ccagatctcg agatcgggca
     1561 gctgcagcga gaacagatca acgggagtga cgcggccgcc gtttggatag ttttccgcgg
     1621 ccgtcacgac ggagggcagc ggactttgcc cacccatttt aggccgcgcg cgagtcgtag
     1681 gcgctaacgc ctgctcgcgg tgcacctcgc gagcgtagcc gcgctgccgc tcgatatcct
     1741 ggccaccgcc tccgaaacgc tcattcatgg tgcctgctgc ccttctgtgt ccgagctcgc
     1801 gctcggactc gctgcgctct tcagttcgtc ctctacggac atcacagcct caattgcacc
     1861 gactaggcgc gtctgctttt cgggcgaaag gctcgccgtc caccgcgcga gcggtccagt
     1921 cgtgcgccgt tcgatcgtgt ccttcagctg cgcaaacacc gtcggcacat tcgagagcag
     1981 cattttgaaa aactgatctt tctgctcggc gctcgcgttc agctgtttct cgactagcga
     2041 gaactcgcga tcgtcgcgtc gcgcgtcgcg ctgttcatcg tgcagacggt tcaagcgttc
     2101 ttcgagctcg cggatccgct tgtaggccgc acgcagctgg tccagcgcca gcttgaacat
     2161 ttcgcggctc ggcaccgcga taagcgagta tgcctcgtgc tgccctttgc gtgcgtccgt
     2221 cgcgacctcg cgcaggtcgg tcaaccctcg cgccatgagt cgcgagagct cgactagagc
     2281 gccgtcgcgc gcgccaccgc cctcgcgttc taggtcgctc tcctcgaagt actcggcacc
     2341 ggggatcccc gaatcggagt catcgtccga gctcgagctc gcgctctgct cgttttgcgg
     2401 cctgctctgc gcgtaggcct ccacgctgcc cggctcgaag cgccgttgga gccggccgcg
     2461 gaacatccca tcataaaacg tcaaatcgcc attgagcgcg aggcggtaga cgtgctgccg
     2521 atgacagcgc aggcgccgcg cgacctggac cgcagtcagc cacgtgtccc agccgtcagg
     2581 ctttccgctg ccgttccgtg tccggttgct gttcgtcgat tgtgtcgggc tcgggtcttc
     2641 cactggcatt cccttccaat ggcgggggca ggatcgatcg tgcgtaggcg ctcgcgagcg
     2701 ccgccgaagc gagcgccgcg agctgcaaag ctaagtctgt cgggggctct tccccgaagt
     2761 cggggagctc aaaggggagc ggatccggtg cggcggatcc ggcggcgtga gtcgttgcgg
     2821 cgtgtccggc ggcagactcg tcagatcttc cggcgccagc tggcgggggg gctgaagact
     2881 gacgaaccgc gcgcgccagt tgctgagcgt gctgacaaag ttcagagcgt cctttgctcg
     2941 ttgccgggat agcgatcggt tctcgcgcca aagcatcgcg atcactcccg agagcgctac
     3001 cactacgctc gcgagcgtcc cgcaggctgt catcagcgcg gtttctaggt tcatccgtca
     3061 cgcctgctgc tcgcgcgacg cgaggccgta gatcgtcgcc cacgtctccg gaccaaccac
     3121 gcccgaaggt tcgagtccgc gctctgcctg ccacgcgcgc agcgcgcgct cagtgagcgg
     3181 accaaatatt tcgtcggggt cgcatccgat ttcgaactgc agaatcgcaa cgacatcacc
     3241 ccgcacgcct cgccgcaatt cggggagcga tctgcagagc cgctccaacg aagcccgcgg
     3301 cgacgaggaa tccgaggatt ccgagccctc cggtcgcaac tcgctgtgtt cggtctggct
     3361 cgcgttgcgg actaaagggc ggtcaaagag atcctctcct gtgtccgctc ggatatcctg
     3421 atacatgcgt tcgagcgcgg ccgcgtaatc tgcgatcgcc tgctccggtg gcttgacccc
     3481 gaggaaatat cgattgaacc gcatcgcagc ggcgagatcg ggaattgagc gccgctcgag
     3541 cgcggccgca acgttcgcgc gccgcggctt cttgggattc gcgccctcga agaggagttg
     3601 gagcgcgagc ccgcgcgcgc cgtcgcgggg tgtcgcgtac ttctggaaat gggtcacgta
     3661 ctgctcgacc ttcccggtct ttggattcca tttcgagtcc tcgtgctcga aggtatcggg
     3721 tccgccggtg ggcctcgtga tcgctcccca attgttcgag ccgaccgagc cggggcgctt
     3781 ccagcccatg ccatagcccg actcgtgttt gcccacgagg cggacggcga ggctctcaac
     3841 cggtgagagt ccgagctcgt ccacgagtga gtccacgatc ttttttgcct cgcggtcaga
     3901 gatgcttgcc attagccccc ctcttcaggt cgcgacgtag atccgctgcc cagtgatcac
     3961 gtatccggat ttggtcacgg cgtggccgcc ctgaaccagc gacgcgcgca cgccagcggg
     4021 cgccctgccg acccaaacgc gccggccctt gatcggcttt ttgccgggca ccacgacggc
     4081 gccgctcggc agctcgacag actgccccga gttgaccgcg ttcagcgtgc gctttcccag
     4141 cgcacttcct ccagctgcga gagtgagggc gagctgcgcg ggcttagggc tcggtgcata
     4201 gcgtgacgtc ggcacctgcg cggccgcggc gggaacgcgt gcgacatgac gagcgggcgc
     4261 ggcaacgggt cgcggcgcgg gtttcccaac cttgcgcgcc ttgcgctgcg cgatcgcgac
     4321 ctgcattgcg ttgaccgcgt gcgcggcaga gcgatttccg ccctcggcct gccgcttgag
     4381 cttggaaatg ttcgcgttga ccgtcgagat cgcgaccttc ccggcctgca ctctgtcgat
     4441 cgctaggttc gccgttgcga ccgccgcggc cgcgggcact ccgaccgcgg gaaatgcgac
     4501 cgcaacgact cccaccgcgg ttttagtgat cgggctctgc acaatcgccc tgctcacgtt
     4561 cgccgtcgcc ttagcggcgc tcgaaacacc gcggcctatg ccctttacga gcttcccgaa
     4621 cgagaaaccg atctcgtccg gacctacgcc gatcgcggtc tcatcaatgc caatttccca
     4681 gccgtccatt tggaatgtcc ttctgttcgg gttccctgtt tcgggcgcct tttgccccag
     4741 tcagctttcg cccagcagtc gcagatggat ccgcgctgcg cgccggtagc cgtagcggcg
     4801 catggtctcg gccgtcgctc ggatctgggc tgggtctccg ccgagcttgc tttgcgtcgc
     4861 gtggaatttc agccaatcgg gcggcgcggg ttcccagcca atctcaccag caacggcggt
     4921 gccgccggcg gcccggttct cgtccaacat ggtcgcgtag gcgcgaacgt tgttcgcggt
     4981 gcgcggcatg ccgagggttt tctcgatgta atcagcgctc tgccgcgcga agctgatcga
     5041 attcttcgcg atcaaactct gaatctggaa gcgcgcggcc gcgagcctct cagtgttcgc
     5101 gagctgctgc gcctcgctgc cgtcaatcag accctcatct agggtcttca ccttggtctt
     5161 ggtcggtcgc agcagaagcg cgccgatcgc tagggctcca atgaaaaata tcggtcccat
     5221 gtctccctca cttgttgggg tcgttctcga ccttgctcgc tgcgcgccag tcggcggcgc
     5281 gcgctggatc cgccacgctt tgagcgatca gcgccttgga ataggcggac ttatccgctt
     5341 tcgcgctcga gctgcgccag tagaaggggc gcggcggaac aattccgtag ctcaccagcg
     5401 caagcgccga tttgtagcca tacagcccgt caaccaccaa accctcttgg ctctggaatg
     5461 ccttcaccag ggcctgatct tccttgtagc ggctggtgtt ctgtaggtgc atcgcgacct
     5521 gctgcgcgag cgcgaggcgc tgcgcctgct gcgacgtaat cgggttgccc ggcggtagcg
     5581 gtaccgggct cggaccaacc ggcggcgtga cgacaggcgg caggacgatt tgcggcggcg
     5641 tgacgatcgg cgggttcgga accgcggtgg acggcgcgac ggtgccaacg gtgcgcagcg
     5701 ctgatgccgt tacgcgcagc gcggcggctt cggttttgag ccccgccgca tccatttcgt
     5761 tcgcgatctg catgagcgcg tccgggctgt tcctgctgag tgcgtccagg atccgcgcct
     5821 ggaaatcgat tgcggttttg ccccccgctg gcgtggtggt gctcggcacg aagccgcccc
     5881 ccgccggcgg cgccgtgccg ccgcccggcg agctcgagct cgagctcgcg cccttcttgc
     5941 cacccgtgaa catgagagca acgagacccg cgccgagcgc gagcggtaac accgggatca
     6001 tgctgacacc ctttcgatag gcaccggcgg aggcacgagg cccgaccgac cggggaaaag
     6061 ttggtaaacg tagaacgcgc cacgcggcac gtattcgccg ggctcgttcc ccatgccgag
     6121 ctcgcgcgac gggtcttccg tcgtcccgtc ggctcgcaaa actgtgatgt gatagaggta
     6181 gcggcccgtc ggtagacggg tccaggtgat ctgcggtcgc gccagctcac cagtccgcac
     6241 gatacgccac gaggccaccc cgcacgcgag atcttcgcag tccccgaatc cgcgctcgag
     6301 caccgtgcgc acgtcgcaaa actgctcgtt tcctggcgcc tcgcgacgat agacgcaccc
     6361 cgcatcatag acgctcggca gcgtcggatc gtgacggtat gcccacacat tcgctatgat
     6421 cagactacgc agcccggacc gcacgaactc cgcgtaatcc cgcgggtcgt ctccgaggag
     6481 cgctcctaag cgaatggtga agccgcgcgc cattactcgc gatatccgat caaataaccg
     6541 gttggagtga ctgacgcggt gtgcgtgttc gtgaagctga gcgagaactg ctcgttaggg
     6601 tttgcggtgt cccattcctc gaaaaacgtt tcgcccgcgt gagtgaagat ctcgcagccg
     6661 gttgagccag agttcgcttc ctgcggacgg ccccgaatcg tcgcgccgtt ataaagcagc
     6721 ttgtcctgat tctgcccgga caggcgcatc ccgatcgcct tgaaagcaaa gttcagcttc
     6781 atgtcgagag gcgcggtcgc gccagcgccg atcgccacgc tcggcaaaaa cgccttcgcc
     6841 cgaatggctg gcgtgccctt cgccggctgc tgtcgctgcc gcgctcgagc cgcgcccgtt
     6901 tccggatcgt tctctcccga cacagtcccc gagtctaaat agatatcggt cgcgccggtc
     6961 tcgtcatcgt agaggcccat tggtggtgta ttcctttcgc tatctcccga gctcgagatc
     7021 gagctcgctg agtcgtttcc cctcgccccg cgtgcgcgcc ccgcgttgct gaaaacatgg
     7081 cccgctgccg ctgccgcgac acctccgacc atgctcaaga gtagagggcc cacgctgctc
     7141 gcccttcagt tcttggccgc gttcttcttc tcgtgctctt cgagcacgcg accgacgaga
     7201 gcggccacgc gcgcagcaag catgcccccg ccgaacgtcc cgagctctgc actgtgtttg
     7261 ccggccattc caaacgccgc ggcgccaaca cacgcaaacc cgatcatggt gtcgatctcg
     7321 aaactcgtgt ccccgaattt cgcgctaccg ggtgtgccgt cgccgaattt tgaccagacg
     7381 acgcccgcga aagcgccgcc cgcaactgtc atcacgcagc ccgtgaggcg ctcggttgcg
     7441 agctcgccgg cgccctgcat cgcctttaat ttgcgccggt acgactcgag gcgtgattca
     7501 gcggcctcga gtttttgagt gggagtcatc gcggtttcgg ccattgtcgt ttcgtccttt
     7561 cgctgtgttg gttcaccgcg cgcgcgcggt gcgcgacgca gctaggaggt tgaagaaaag
     7621 gagcgcgccc gcggccagcg cgagcacgcc cacgatcgag cctgtggttt tggcgacgtc
     7681 ctttgcggag tcgatcgccg agtcgatcgc atcatccacg cgatcgatcg cgtccggttc
     7741 cggcggtggc ttcgttacgg tgtcgccttc ggtcgtctca gtgccgcgct ctgcgatcgt
     7801 cggataaccg aatttgatcg catcccatgg cacgggctcg cgcacctcga acagcgccca
     7861 aagccgcgag cggccgaaat caggcgcggc ggctgcgtcc gctgcgagct tcgcgagcag
     7921 tggattgatt acccacccgc tatttgcgat cagctccgca aacggatccg cgcctggccc
     7981 gtggtacacc gtttgccgaa tgagcaccgc gttcttgtgc gcagatgccc aggcgcgaaa
     8041 ctcttcgatg gtgccgccgt gctcaccgat cgccggcgtg aaaacatcct gccagtacac
     8101 ggcgcgcggc agcggattgc ggcgctcgat cgtcatgcgg cacccacgag atcgagcgcc
     8161 caaacgcaat catgcggcag ccactcgccg cgcgtatcga gcaccggcca gggcatcgca
     8221 tctgcgccgc cgaaatagag cagctcgagc gcgacgctcg cgaatgtatc cccggccatg
     8281 gtgagccgcg gccgcaaccc gtcggacgta gcagccaccg agcagtaatc gggcagctcg
     8341 atcgccctcc gtctcactac cccccgcacg ttcgcgatga tttgcgcgcc ccatggctgc
     8401 tcacgcgcaa accatttttc gagcgtgacc ctcgcccctt cgaggatctg atcgttgctc
     8461 gggtgatcga gcagcgtgcg acgtagcacc caccaattga gcgaaccacg ccgcaacggg
     8521 cagcggttcg cccgttggaa cgccgcaaca tgctcacgca gctgcgacgc gccagcgggg
     8581 cgcggcagag tttccacaag cgggcacgct actgcgatcg atcgccctag gcaattgctc
     8641 gtgacacgac gatcgctcgg gtcggtgtct cctcaactcc actccgagga gacaacacac
     8701 caacgagaga gcggttttta tgcgtgagcc gttgcgcgga tctctatgct gtccgcgtgc
     8761 attgcgaatc gctcgcgagc gcgcacctac actctgcgtg ttcgatcgcg ggcgaggtaa
     8821 cgttttagcg cgcggcgcac gatttccgcc actgcgcacc cctcgcgttc cgcctcagaa
     8881 tccaacattt tcgcgagcgc tggcgcgagc acgacctcga ggcgaactcc cgatgtgcgg
     8941 tttccgccac ccacacgcgc gagcttttta cggcctacgg gaagcatcag atcttaccgc
     9001 cgagccgcgc gaaaaatagc aaagcgcgct cccccgcgtc gcacgctggg catagcgcgc
     9061 aaggctcgag cgtgagccgt agcgctgcgc agctcgccgc gcagagccgc tgtttcgcgc
     9121 gttcgatgcg cttctgttcg gacgcgagcg gcgggcgcgg agacatttca cgcatttcgc
     9181 gagtctccca tttttgccac cgtcttccta gttcccggtt ttttcgcgtg aaagtgcgtg
     9241 ccccctgctc tccggagatt ctccggagaa cacttgcgcc taccaatctc aacccgcgag
     9301 ggcgcaaagc ctagacgtca cgccgtagcc tatgccatga cccggaccgc tcggcgtggc
     9361 gcgacagttc cccccccggc tgccgtcgcg ccgagcagat tacccccggg gaagggcgga
     9421 cttgtgggaa tgaggaaatt cggactgtgg gcgttcgcgt tcgtgctgct gcttgcgtgc
     9481 tctgaggaac accctccgga cgcaccgagc agcatgccga ctggaggccg cgcgagcggt
     9541 ggctcgagct cggccggcgc cagcgagcag ccgagcgacg gcggcagcgt ggcagagcca
     9601 gagagcggcg gccgcactgc aacaacgggc ggcagacgag caagcggcgg cgcgagctcg
     9661 gcggggtcgg ttggttcggg cgggcgcgct ccgacgggcg gcacgactgc cgagggcggc
     9721 gcgagtgagt cggagggcgg ca
//
LOCUS       DASTKS010000286         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_268327, whole genome shotgun sequence.
ACCESSION   DASTKS010000286 DASTKS010000000
VERSION     DASTKS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268327"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..1433
                     /locus_tag="VFQ35_05975"
     CDS             522..1433
                     /locus_tag="VFQ35_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930210.1"
                     /translation="MRFIAHSLVLFSLLGVACAQKEAPVAAPSGEQAGFAERYPARLN
                     GARTHFASDENQARATLGEFKGYPDALKNPDWNSVHTLVEQADSTGKSSAYTEAALEA
                     ETVTRFFDEEKDGLRQKVGGSVSYASTQKKCEEDLGGTAVAAMERGVDKQLEERMRAH
                     SEAHRYIEDHEDELGKQNVETLKKQADKIARTSNIAYVRLELYRREVEILLNDDSNVR
                     STLDRTIQESDATLANASASKSKKAAAQKRKDAAQKARAALDAEVEQDRRGLEEMQQR
                     INTLQNDYRAALDALLADVKTRADMKK"
     gene            1713..2390
                     /locus_tag="VFQ35_05980"
     CDS             1713..2390
                     /locus_tag="VFQ35_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity nickel-transport family protein"
                     /protein_id="HET9930211.1"
                     /translation="MTWLTALALGFLLGMRHATDADHVVAVATIVARERRILAALRVG
                     TLWGIGHTTTILLVGGAIVLFGLVIPPELGLSLELSVAVMLVLLGGLNLLWALRDPAA
                     DANSKQDVPTVPMPGRRLQPLLVGSVHGLAGSAAIALLVLTTLRDTTFALVYLAVFGL
                     GSVLGMALLTSAMAAPLAFASRRFASFTRRLSGISGAVSIAFGLFLIYQIGFVDGLFN
                     ALAYGSR"
     gene            2607..3137
                     /locus_tag="VFQ35_05985"
     CDS             2607..3137
                     /locus_tag="VFQ35_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930212.1"
                     /translation="MSRALLSFVLLSLFTFSCGGGESAPAKSADDVAAESSGASSSDN
                     DGASAKPASDDEAGEGSKPKAKSDEPSAAEPQFTEGMSVDEAIKAVPAGIERRNIDQE
                     TLGKPLQDVSVYEPCKPGSAHVKIKVAVWDGRAVGVDVTSTPKNEKLSSCIKQKISEL
                     KWEKKVKSLNTIEYQL"
BASE COUNT          749 a         1408 c         1092 g          571 t
ORIGIN      
        1 acgcccacgc cgaaaagacg cgacgcgccg ttgcgacggc gcacgcgacc accaccgcca
       61 cggagaccgc acgcacgcgc cacgccgaaa agacgcaact cacgccgatg cgacctccac
      121 cacgcacgca cccgcacccg cgcggaagac ccgacgcatg ccgatgcgac cgcacccgca
      181 cccgcaccgc gcgaagacgc gacgcacagc gctgagtccc tcaccgcgct gcgctgcacc
      241 cgcgcccgca ctcgcgctgg cgcggaagac ccgacgcatg ccgatgcgac cgcacccgca
      301 cccgcaccgc gcgaagacgc gacgcatgcc gatgcgaccg cgcacgcgcc ggagacgcga
      361 cgcacagcgc tgcgcccgca ctccacgacg aattccgagt gccgcagcgc agcgtgactt
      421 tcggaaacag aaaaattttt gagtcaatcg cgccgccgcg cgccgaacag cgggcctggg
      481 ggttgactcg cgctcacaac cggtagaaat gccgacccgc aatgcgattc atcgcgcact
      541 ctttggtcct cttctcactc cttggagttg cttgtgccca gaaagaagcg cccgtcgcgg
      601 ccccttctgg cgaacaggca ggctttgccg agcgctaccc ggcgcggctg aacggcgctc
      661 gcacccactt cgcgagcgac gaaaaccagg ctcgcgcaac cctcggcgag ttcaaaggct
      721 accccgacgc gctcaagaac ccggattgga actcggtgca caccctcgtg gagcaagccg
      781 acagcaccgg caagtcgagc gcttacacgg aagcggctct cgaagccgaa acggtgacgc
      841 gcttcttcga cgaagagaag gacggcttac gtcaaaaagt gggcggctcg gtcagctacg
      901 cttcgacgca aaagaagtgt gaagaggacc tgggcggcac cgccgtcgcg gcgatggagc
      961 gcggcgtcga caagcaactc gaagagcgca tgcgcgcgca cagcgaggcg catcgctaca
     1021 tcgaagatca cgaagacgag ctcgggaagc aaaacgtcga gaccttgaag aagcaggccg
     1081 acaagatcgc gcggaccagc aacatcgcgt acgtgcgttt ggagctctac cgacgcgaag
     1141 tcgagatcct cttgaacgac gactccaacg ttcgttccac cctcgatcgc accattcaag
     1201 agagcgatgc gaccctcgca aacgcgagcg cctccaagtc gaagaaagcc gccgcccaaa
     1261 agcgcaaaga cgccgctcaa aaggcgcgcg ccgcgctcga cgccgaggtg gaacaagacc
     1321 gccgcggcct cgaagagatg cagcagcgca tcaacaccct ccaaaacgat taccgcgccg
     1381 cgctcgacgc cctcctcgcc gacgtgaaga cccgcgccga catgaaaaag tgagccccgc
     1441 cggccccacg ccaggcgccc cgcctccgaa acctgcccgc cacatgaggc ggcccgcttc
     1501 cccgacgtta gccacgcccc gacgttagcc gcgccccgac gttagccgcg ccccgacgtt
     1561 agccgcgccc cgacgttagc cacgccccga cgttagccac gccccgacgt tagccacgcc
     1621 cccaatgctc gcggagcgtc ccccggcgct cgcagcgcac caggggcgct cctctcagca
     1681 cgcccacagc cagtcgtgct aggacgagtg atgtgacgtg gctcacggcg ctcgcgctcg
     1741 gcttcctcct cggcatgcgc catgccacgg acgccgatca cgtcgttgca gtcgccacca
     1801 tcgtcgcacg cgaacggcga atcctcgccg cgctgcgtgt cggaaccttg tggggcatcg
     1861 ggcacaccac caccattctc ctggtcggcg gcgcaatcgt gttgttcggg ttggtgatcc
     1921 cgccggagct cggcctctcg ctcgagctct cggttgccgt catgctcgtg ttgctaggcg
     1981 gactgaacct gctctgggcg ctccgcgacc cagcagctga cgcaaactcc aaacaagatg
     2041 tcccgaccgt gcccatgccc ggtcgccgcc tgcagccact actcgtcggc tcggtgcacg
     2101 gcctggccgg ctccgctgcc attgccctgc tcgtcctcac caccctgcgc gacaccacgt
     2161 tcgcgctcgt ttacctcgcg gtcttcggcc tcggcagcgt ccttggcatg gccttgctca
     2221 cgagtgccat ggcggcgcca ctcgccttcg cgagtcggcg cttcgcctcc ttcacccgtc
     2281 gcctctcggg gatcagcggc gccgtcagca tcgccttcgg actcttcctc atctaccaaa
     2341 tcggcttcgt cgacggcctc ttcaacgccc tagcctacgg ctcccgatag ccgacctccc
     2401 ccgcaagcgt agccccgaaa acccctgctc caggcactgc tcgacattcg gccgccgccg
     2461 ccccaacgaa gcgccgcccc gacgaagcgc cgccccgacg aagcgccgcc ccaacgaagc
     2521 gccgccccga cgaagtgccg ccccgacgaa gcgccgtcta gacgacctct cgaccggact
     2581 cgggtgctgg agtagagtcc tcgatcatgt cgcgggcgct cttgtccttc gtcctgctct
     2641 ctctctttac gttctcctgt ggtggcggcg agtccgctcc cgccaagtcc gcggacgacg
     2701 tcgcggccga gagctccggg gcctcgagct cggacaacga cggtgcaagc gcgaagcccg
     2761 cgtccgacga cgaagccggc gaaggctcca aacccaaggc gaagtcggac gagccgtccg
     2821 ccgcggagcc ccaattcacc gaaggcatgt ccgtcgacga agcaatcaaa gccgtgcccg
     2881 ccggtatcga gcgccgcaac atcgatcaag agacactcgg caagcccttg caagacgtct
     2941 ccgtatacga gccctgcaag ccgggctcag cccatgtgaa gatcaaggtt gcggtgtggg
     3001 acggtcgcgc cgtgggcgtc gacgtcacca gcactcccaa aaacgaaaag ctctcgagct
     3061 gcatcaagca gaaaatctcc gagctcaagt gggagaaaaa ggtcaagtcg ttgaacacca
     3121 tcgagtacca gctctaaagc cgctctaaag cgtctcgagc cccgctccag gagtgctcga
     3181 gcttcctacc cccacgtcga acagcacggc tcatccgccg tcgtgcactc gccgccgtat
     3241 ctggcgtcgt gcaagacgcg ccgctcaagc agcgtcgcgc aagacgcgcc gccgtatctg
     3301 gcgtcgtgca agacgcgccg ctcaagcagc gtcgtgcaag acgccgcgca tcgcggccca
     3361 ttgaggcgac gccgcgcatc gcgccgtcgt gcgagggcgc cgctcatcgc gtcccgctca
     3421 tctccgatac gtcgaaacac aatctcatgg cgatgcgtcg gaacacaatc tcatggcgat
     3481 gcgtccctac gttgccgatc accacgacct cgaaacacaa tctcatgacg atgcttcgat
     3541 atccgcgcac tctctcgagt tcgtgcttga cacgatttta gagcatcggt ttgcgctctg
     3601 cgcatcagag tagtttttcc accgattgcg tcgagcacgc ggccgtttac gccgtcgttc
     3661 ctagggtcga cacttcgaaa gtcgttcctg tgcgcgcgag cgcgtgagcg agcaaatacg
     3721 aagctccgaa aaccagggtg taaggtcgag cgcatgagcg ccgccaacgt cgcgagtgga
     3781 ttcgattcag tttctcacat cggttcagtt gctcagatcg
//
LOCUS       DASTKS010000287         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_492989, whole genome shotgun sequence.
ACCESSION   DASTKS010000287 DASTKS010000000
VERSION     DASTKS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492989"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..902
                     /locus_tag="VFQ35_05990"
     CDS             153..902
                     /locus_tag="VFQ35_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9930213.1"
                     /translation="MENSRTDFRYYTPDYEFLTSDTWRGDIALPQGSARLEPGVLVCL
                     RPGDVCSGQVADPGSRTSLHIERELLEELLAECGLSLDAACLRRSTRMSAHLEARLRA
                     VHAAIAPDCTVLEIRAALVEFVAALASEAVEEEPPPSSAPSSAKDAADAVRRCLEHDP
                     STNVDLAAVARETGMSRFRALRVFKRWYGLPPHTYQLHVRVCLVQKSLRSGSRPAEAA
                     AEYGFVDQSHLTRHFRRLLGITPAVYARIGS"
     gene            complement(909..1367)
                     /locus_tag="VFQ35_05995"
     CDS             complement(909..1367)
                     /locus_tag="VFQ35_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930214.1"
                     /translation="MKLHAIALWIIGPLAVGAASTLLGASAAKSKDAVVRDTASLGAA
                     ERKHLETLPGRAISDAAWALADVDTARKVLQRELDRLPESDGPARARTFIRFGIIDPN
                     PDGQAAVFSLACVEDPSVCDRMKEAGERETVVRLVAPGNHLPLSLLGGHP"
     gene            complement(1364..1720)
                     /locus_tag="VFQ35_06000"
     CDS             complement(1364..1720)
                     /locus_tag="VFQ35_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930215.1"
                     /translation="MQRGLLAAAYLGAALVGYLLGGEQAYRRALAASSAAARVGFALR
                     TSAPVSAQLGYDVERVREELKADERDLLDLVVATRGLTRGGEPDWAAAEAVCRSNGHL
                     RCDRPALERLRLGSYR"
     gene            1803..2771
                     /locus_tag="VFQ35_06005"
     CDS             1803..2771
                     /locus_tag="VFQ35_06005"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9930216.1"
                     /translation="MSNTELAPLFRVEGGPVLPAGSADWVIARSGRRLRAALFEPPVE
                     RRGSIVLSPGRTEPIEKYGEVVSDLVGRGFVVLVHDWAGQGLSHRFQSDRMRGDIEGG
                     ADAFVADYGDVVGAFALRLPRPWIAMGHSMGGALTALALTESDLKFDAAALCAPMMEF
                     SAGGLPIALAEVVVGCALRLGLGKRLPRPQADPVEVPFETNVLTHDRPRYERTLALYR
                     ACPDLRLGEPTWRWLSFGLDLRARLALPGAAERISVPVLVVGAGADRVVHVAPIRRFS
                     DRLPRGKYLELAGAFHEILMERDEHRNRFFDAFDDFAAEIVAQRAR"
     gene            2914..3312
                     /locus_tag="VFQ35_06010"
     CDS             2914..3312
                     /locus_tag="VFQ35_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930217.1"
                     /translation="MAEKDKVDTKTLPGLGHPLEATVGTLGAVSGAGFGAIAAGPAGA
                     IIGGLIGAAVGATTGWAADENTVEKIEADKALDDEIGVTSGSLGAPNLEHPEPRIAAP
                     SAASAGASVGAPQTAQDADGPLGNAVNDEV"
     gene            3727..>4920
                     /locus_tag="VFQ35_06015"
     CDS             3727..>4920
                     /locus_tag="VFQ35_06015"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET9930218.1"
                     /translation="MLRFVVALVVGVSVASCAGGRAPSVSRDVTFAELRTIKGTPRVQ
                     SPGEAARTPYARERLVDGEQVTLEAGALAWMRRDGGATWLIAGPAEFTLHADDVELKQ
                     GRVFVDNELGGAVEALTPRGKVELSEARASIDIATDGTMSVYVLRGSARAAGHERASA
                     GELLSWQTDGKLRREASRAWDDWTGGLATAEPAAEPAPFGLGTVGARKPGDSGQPRFP
                     LVIQRLDVKVRVDRDFAVTEVDETFVNPSSDVVEGLFSFRTPKNSVLSRFGVDRQGSI
                     VWGRIQESRSAAKQYASNVYAGSEEEPALLQWAGGGTYNARLYPIRPGQSRRVVTRYT
                     EWLSRQGPQGERRLYVYPMAAEGARASLPRIEELRFTLDLSNAGALRVHSGLQGKREG
                     NQVVVK"
BASE COUNT          805 a         1551 c         1689 g          875 t
ORIGIN      
        1 ccgcgaaacc cttgaccttt tcggggagtt cgcgcgccgt cggctcggac cgcttcttgc
       61 catgggcggg tgagatggtg agggtcagca ataggactga atcggtctca tttcggcgcc
      121 gccgcgaaat cccaggggtc gaggttcggc gggtggagaa ctctcgtacg gattttcgct
      181 actacacgcc ggattacgag tttttgacgt ctgatacgtg gcgaggtgac atcgctttac
      241 ctcagggttc agcgcggctc gaaccgggcg tgctcgtgtg cctccgcccg ggcgacgtct
      301 gctctggcca ggtcgcggat ccgggttctc gcacgtcgct gcacatcgag cgcgagctgc
      361 tcgaggagct ccttgcggag tgcggtttgt cgctggacgc ggcgtgcctg cgacggtcga
      421 cccggatgag tgcgcacctc gaagcgcgtc ttcgcgccgt tcatgcggcg atcgctcccg
      481 actgcaccgt tttggaaatt cgggccgccc tcgtcgagtt cgttgctgcg ctcgcgagtg
      541 aagcggtgga ggaggagccg ccgccgagca gcgctccgag ctcggcaaag gatgctgccg
      601 acgcggtacg acgctgcttg gaacacgatc cttcgaccaa cgtcgatctc gcggcggtgg
      661 cgcgcgagac cgggatgagt cgcttccgtg ccctccgcgt cttcaaacgc tggtacggat
      721 tgcctccgca cacgtatcag ctgcacgtgc gggtctgcct cgtccaaaag tcgctccgct
      781 cggggtcacg ccccgccgaa gctgcagccg agtacggctt cgtcgatcaa agccatttga
      841 cgcggcattt ccgccgcctg ctcgggatca cccccgcggt ctacgcgcgc atcggttcgt
      901 gaggcgccct acgggtggcc accgagcagc gaaagcggga ggtggttgcc cggcgctacg
      961 agacgaacga cggtttcgcg ttcgccggct tctttcatgc ggtcgcatac gctcgggtct
     1021 tcgacgcagg cgagcgaaaa gacggcagct tgtccgtcgg gattcgggtc gatgattccg
     1081 aagcggatga aggtgcgagc gcgcgccgga ccgtcggatt cgggtaggcg atcgagctcg
     1141 cgttgaagca ctttacgggc ggtgtcgacg tcggcgagcg cccacgcagc gtcgctgatc
     1201 gctcgcccgg gaagcgtctc caaatgcttt cgttcggcag ctccgagcga agccgtgtct
     1261 cgaacgacgg cgtccttgga cttcgcggcg gacgcgccga gcaaggtcga ggccgcgccg
     1321 accgcgagag ggccgatgat ccaaagggcg attgcgtgca gcttcaccga tagctcccca
     1381 gtcgaagtcg ctcgagtgcc ggccggtcac agcgaaggtg cccattgctc cgacaaaccg
     1441 cttccgccgc ggcccagtcg ggctcaccgc cgcgcgtgag cccgcgtgtc gcgacgacca
     1501 agtcgagaag atctcgctcg tcggctttca gctcctcacg cacgcgctcg acgtcgtagc
     1561 cgagctgcgc gctcaccggg gcactcgtcc ggagtgcgaa tccgacgcgc gcggccgcgc
     1621 tcgaggcggc cagagccctt cgatatgcct gctccccgcc gagcagatag ccgaccagag
     1681 cggcaccgag gtacgctgcc gcaagcaatc ctctctgcac atagccgaat cgcgtcaaag
     1741 cgcggctact ctacgtctct cccgagcctc cccgcaacgc gtccaatcag ctccgaatat
     1801 tcatgtccaa tacagaactc gcacccttgt ttcgagtcga aggtggtcca gtgttgcctg
     1861 ccggttcggc cgattgggtg attgcgcgct ctggtcggcg cttgcgcgcc gcgttgttcg
     1921 agccgcccgt ggagcgtcgc ggctcaatcg tgttgagtcc gggtcggacg gagcccatcg
     1981 agaagtatgg cgaagtggtg agcgacttgg tcggacgcgg cttcgtcgtg ctggtgcacg
     2041 attgggcggg ccaaggtctg tcgcatcggt tccaaagtga ccgcatgcgc ggcgacatcg
     2101 agggcggcgc agacgcgttc gtcgcagact acggcgacgt cgttggcgcc tttgcgcttc
     2161 ggctcccgcg gccttggatt gcgatggggc attccatggg gggtgcgctc acggccctgg
     2221 cgctcaccga gagcgatctc aagttcgacg ccgcagcgtt gtgcgccccg atgatggaat
     2281 tctcggccgg tggtcttccc atcgcgctcg cggaggtcgt cgtgggctgc gcgttgcgtt
     2341 tggggctcgg taagcggctc ccgaggcccc aggccgatcc ggtcgaggta ccattcgaga
     2401 ccaacgtgct cacgcacgat cgccctcgct acgagcggac gttggcgctc tatcgcgcat
     2461 gtccggatct gcgcctcggc gaacccacct ggcgctggct cagctttggc ctcgacctgc
     2521 gcgcgcgcct cgcgctgcct ggggcggcgg agcgcatttc ggttcctgtg ttggtggtcg
     2581 gcgccggtgc cgatcgcgtc gtgcacgtcg cgccgatccg ccgcttcagc gaccggctgc
     2641 cacgaggaaa gtacctggag ctcgcggggg cgttccacga gattttgatg gaacgtgacg
     2701 agcaccgcaa tcggttcttc gatgcgttcg acgacttcgc cgctgagatc gtcgcccagc
     2761 gtgcacgctg aagcgagtcg gcgtgcgctg tcgagtcagt gcagtgaggc gtcctggcac
     2821 atgaagagca gcgctcgagc gctgcgaatg cattggcccg ttgaatgcat cgatgtccga
     2881 gctcacgcgc agcatgcgtg aaaggaagtc atcatggctg agaaggataa agtcgataca
     2941 aaaacgctgc cggggctcgg tcatccgctc gaagcgaccg tgggtacgct cggcgctgtc
     3001 agcggtgccg ggttcggtgc gatcgcggcg ggtcccgctg gagccatcat cggcggattg
     3061 atcggcgccg cagtcggtgc caccaccggc tgggccgcag acgagaacac cgtcgagaag
     3121 atcgaagccg acaaggcgct cgacgacgag attggcgtga cgtcgggctc gctcggagcg
     3181 ccgaacctcg agcatccgga gccccgcatc gccgcaccgt ccgcggcgtc ggcgggagct
     3241 tcggttggcg cgccgcagac cgcgcaagac gctgatggtc ctttgggcaa cgcggtcaac
     3301 gatgaagtct aattgggcgc gaacggggtc gagccgctcg gccccgtacc acgcccctcc
     3361 gatgccgcgg cggcgcaact gctaagacga gtgacgagct caggggagtg tgcggctgtc
     3421 gggcgaacgc cttcacgcaa cctgtgaatg ccgacgatga gctctccgcc gagcggagaa
     3481 accaagagga cctgttcctc gagggacgaa atgagctgct ttcgggtgta agcgcggacc
     3541 gacggcgccg ttgacgcccg catgcggcga cggaccggcg accgaaaact catggggagc
     3601 caagcctcgc gaggactcgc gggtagaact gcgagctggc ttgagtcggc ggtttgcgtc
     3661 gggtacatgg aaaaacagga agggttcgag ccccctgccg gcaattctgg gctcgacggg
     3721 taagcgatgc tgcgattcgt cgtcgctctg gtagtgggtg tttccgtcgc gagctgcgct
     3781 ggtgggcgcg cgccgagcgt ctctcgagac gtcacgttcg cggagctcag gaccatcaaa
     3841 ggcacgccgc gtgtgcagag ccccggtgaa gctgctcgca cgccctacgc tcgcgagcgc
     3901 ttggtcgacg gcgagcaagt gacgctcgaa gcgggcgctc tcgcgtggat gcgacgcgac
     3961 gggggcgcga cgtggctcat cgctgggcct gccgaattca cccttcatgc cgacgacgtc
     4021 gagctcaagc aaggccgcgt cttcgtcgac aacgagctcg gcggcgcggt ggaggcgctc
     4081 actccgcgcg gcaaggtcga actttcggag gctcgcgcga gcatcgacat cgcaacagat
     4141 ggcacgatga gcgtttacgt cctgcgaggc tccgcgcggg ctgccggcca tgagcgcgca
     4201 tctgccggcg agctcctttc ctggcagacg gacggaaagc tgcgacgcga agcaagtcgc
     4261 gcttgggacg attggaccgg cgggctagcg acggccgagc ctgctgccga gccagcgccc
     4321 ttcggtttag gcaccgtggg cgcgcgcaaa ccaggagaca gcggacaacc gcgctttccg
     4381 ctcgtaatcc agcgcctcga cgtgaaagtg cgcgtcgatc gcgacttcgc ggtcaccgaa
     4441 gtcgacgaga cgttcgtgaa tccgagcagt gacgtcgtcg agggcttgtt cagctttcgc
     4501 accccgaaaa attcggtgtt gtcgcgcttc ggcgtcgatc gacaaggctc gatcgtttgg
     4561 ggacgaattc aagaatcgcg ttcagccgcc aagcagtacg cctcgaacgt gtacgcgggt
     4621 tcggaggaag agccggcgct cttgcagtgg gcaggcggcg gtacttacaa cgcgcggctc
     4681 tatcccatcc gccccggaca gagtcggcgg gtggtgacgc gctataccga atggctttca
     4741 cggcagggcc cgcagggaga gcggcgactc tacgtttatc cgatggcggc cgagggcgct
     4801 cgcgcgtcgt tgccgcgcat cgaagagctg cgcttcacgc tcgatctctc gaacgccgga
     4861 gcgctgcgtg ttcactccgg gttgcaaggc aagcgggaag gaaaccaagt cgtcgtcaag
//
LOCUS       DASTKS010000288         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_47133,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000288 DASTKS010000000
VERSION     DASTKS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47133"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..786
                     /locus_tag="VFQ35_06020"
     CDS             136..786
                     /locus_tag="VFQ35_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930219.1"
                     /translation="MRKYSGSMDGFGGDLHLPNSSARGLAGADEICTAIAEEAMPGAG
                     AKGWRAFLSTVTGPVHAIDRIGQGPWYDRLGRVVALTKADLLNDRPNGADPAIINDLP
                     NELGIPNHTDGKPGCTGDSCPDNHDTLTGTGKNGQLYKMDMATTCNDWTSKEPSGRPW
                     CGHSWPRQGSGVNWMSSISEGGCAPGINLVEMGPAMTTTVGSGGGYGGIYCFALKP"
     gene            complement(849..2159)
                     /locus_tag="VFQ35_06025"
     CDS             complement(849..2159)
                     /locus_tag="VFQ35_06025"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HET9930220.1"
                     /translation="MHFDQDPLRLARDLMRFIDASPSPYHAVAEVERRLVSERFVRFD
                     ERDSWELSEGTRGYVVRGATLLAFVMGSRPPAEAGFVILGAHTDSPNLRLKPRPELES
                     AGYKQLAVEVYGSPILATWLDRDLGLAGRVVLADGTSALVRLNEPVMRIPNLAIHLNR
                     DVNKEGLSLNAQQHLVPVLGLEAMKPSSVLELVCHGLAANGNTGVMPEDVLGFDLCLF
                     DLAASTLGGSSGEFVFAPRLDNLGSCHAAYLGLIGSAGSERTRVVALYDHEEVGSQSE
                     AGARSRLLESVLDRLAQSWKDAGRDATARAFSRSYLVSCDMAHAVHPNYADKHDKLHR
                     PLLGQGPVIKVNANQSYATDGPAAARFVRACRQSEVVPQHFVSRNDMPCGSTIGPITA
                     ARLGINGVDVGNPMLSMHSCREMAGSGDIGPMVRVLGTLLESAG"
     gene            2184..3170
                     /locus_tag="VFQ35_06030"
     CDS             2184..3170
                     /locus_tag="VFQ35_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930221.1"
                     /translation="MLSPDVRVEGFSAEEWLRIGHVFSSPNAAKPSGPRGGVVALTTS
                     GKLRKLVSTRRGRLEHSAEAWPIDLSELARRHDASWAVELTTGSLDSLADRFAERLRP
                     ADTYLSQTLELLLVLRELEATGQVRVWPWPVAEWPIPTERAVLRAFDALCPVGRVALL
                     GVFSHGSLYSAVALRRGAKGIEAIVGPGELRPHMGLLSGDWQRDYRFLAEATEKVVGP
                     LAVGCFAELYTFQSLVGAAPGSWSLAVASRDVVLSPVTPGLALPLGLDAGRAMWVSLR
                     GFAERFGAAEWLGAAGRLRPSREFGLPAFEADIKAWLGFDPLRLLSRLMTPK"
     gene            3254..>3435
                     /locus_tag="VFQ35_06035"
     CDS             3254..>3435
                     /locus_tag="VFQ35_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930222.1"
                     /translation="MASTPPERGLRGGRVLFADDDPSVRAAIARILRAHGFLVDLASD
                     GTEALAFAREYPYALVA"
BASE COUNT          543 a         1108 c         1187 g          597 t
ORIGIN      
        1 agcggtggct cgacgagcag cggtggctcg acgagcagcg gtggctcgac gagcagcggt
       61 ggctcgagca ctgcaggtgc gggcggtggc ggcgcgaccg acaagttcag tttcttcgtc
      121 acgagtctcg ccgcgatgcg caagtactct ggcagcatgg acggcttcgg cggcgacctc
      181 cacttgccaa actcatcggc gcgcgggctc gccggtgcgg acgagatttg caccgcgatc
      241 gcagaagagg cgatgcccgg cgctggcgcc aaaggttggc gtgcgttctt gagcactgtc
      301 actggccccg tgcacgcgat cgaccgcatc ggacaaggcc cttggtacga ccgcttggga
      361 cgcgtcgtcg cgttgacgaa ggcggatctt ctgaacgatc gcccgaacgg cgccgacccc
      421 gccatcatca acgacttgcc caacgagctc ggcattccga atcacaccga cggcaagccc
      481 ggttgcaccg gcgactcgtg ccccgacaac cacgacactc tcacgggcac cgggaagaac
      541 ggccagctct acaagatgga catggcgacc acgtgtaacg actggacgag caaggagccg
      601 tcgggcaggc cctggtgcgg tcactcgtgg ccgcgccaag ggagcggcgt gaactggatg
      661 tcctcgatat cggaaggcgg ctgcgctccg gggatcaacc tggtcgagat gggaccggcc
      721 atgaccacga ccgtcggctc gggcggcggt tacggcggca tctattgctt cgcgctgaag
      781 ccctgacgcg atcgaaagtg agaagcgatc gtcgcggccg agggccgaag cgacgatcgc
      841 tccgagcgtc agccggcgct ctcgagcagt gtcccgagca cacgtaccat cggtccgatg
      901 tcgccgctgc cggccatttc tcggcaggag tgcatgctga gcatggggtt accgacgtcg
      961 acgccgttga tgccgagccg cgccgccgtg atcggcccga tggtgctgcc gcaaggcatg
     1021 tcgttgcgcg acacgaagtg ctgcgggacc acttcactct gacgacacgc gcggacgaag
     1081 cgcgcggcgg ctggtccatc cgtggcgtag gactgattcg cgttgacctt gatgacaggg
     1141 ccttgcccga gcaacgggcg atgcagtttg tcgtgcttgt cggcgtagtt cgggtgaacg
     1201 gcgtgggcca tgtcgcagga gacgaggtaa gagcgcgaga aggcgcgcgc cgtcgcgtca
     1261 cggcccgcgt ctttccacga ttgcgcgagc ctatcgagca cgctctcgag taagcgcgag
     1321 cgagcccctg cttcgctctg actgcctacc tcttcgtggt cgtagagcgc gacgacccgg
     1381 gtgcgctcgg agcctgcgct gccgatgagc ccgagatacg ccgcgtgaca tgagccgagg
     1441 ttgtcgaggc gcggcgcgaa cacgaactcc ccgctacttc cgccgagcgt gctcgcggcg
     1501 aggtcgaaca agcacaggtc gaagccgagc acgtcctcgg gcatcacgcc ggtgtttccg
     1561 ttcgcggcca agccgtgaca gacgagctcg agtacgctgc tcggcttcat cgcttcgagg
     1621 ccgagcaccg gaaccaggtg ttgctgcgcg ttcagtgaca gcccctcctt gttcacgtcg
     1681 cggttcaggt gaatcgccag attggggatc cgcatcaccg gctcgttcaa acgaacgagc
     1741 gccgaggttc cgtcagcgag aacgactcgg cccgccaggc ccaaatctcg gtcgagccag
     1801 gtcgctagga tcgggctgcc gtacacctcg accgcgagct gtttgtagcc tgcgctctcg
     1861 agctccgggc gcggcttcaa gcggaggttc ggggagtcgg tgtgggctcc gagaatgacg
     1921 aagcccgcct ccgccggtgg ccgcgagccc atcacgaaag cgagcaatgt cgccccacgc
     1981 accacgtatc cgcgcgtccc ctccgagagc tcccagctgt cccgctcgtc gaagcggacg
     2041 aagcgctcgg acacgagccg tcgctcgact tcggcgacgg cgtgatacgg gctcggtgac
     2101 gcatcgatga aacgcatgag atcccgcgcg aggcggagcg gatcctggtc gaaatgcatg
     2161 ccccgactat actccgcccc tcgatgctca gccccgacgt gcgtgtggaa ggtttcagcg
     2221 cggaggagtg gcttcggatc gggcacgtgt tcagctcgcc caatgccgcg aagccgtctg
     2281 gcccgcgcgg gggcgtggtc gcgctcacga cgtccggcaa gctgcgcaag ctcgtttcga
     2341 cgcgccgcgg acgtctcgag cactcggccg aagcttggcc gatcgacctt tcggagctcg
     2401 cgcgccggca cgatgcgagc tgggcggtcg agctcacgac cggcagcttg gactcgttgg
     2461 ccgaccgctt cgcggagcgt ctgcgtccag cagatacgta cctctcgcaa acgctggagc
     2521 tcttgctggt gctgcgcgag ctcgaggcga cggggcaggt gcgggtttgg ccgtggccgg
     2581 tcgccgaatg gccgattccc accgagcgag ccgtgttgcg tgcgttcgac gcgctgtgtc
     2641 cggtgggtcg cgtggcgctg ctcggcgtgt tctcgcatgg ctcgctgtac agcgcggtcg
     2701 ctttgcgccg cggagcgaag ggcatagaag cgatcgtggg tcccggtgag ctccgcccgc
     2761 acatggggct tctttccgga gattggcagc gcgactatcg cttcctggcg gaggcgaccg
     2821 aaaaagtggt ggggccgctc gcggtcggtt gttttgccga gctctacact tttcagtctc
     2881 tggtcggcgc cgcgcccggc tcgtggagcc tcgcggtcgc ttcacgcgac gtcgtgctct
     2941 cgccagtcac gccgggtttg gcgctgccgc tcgggctcga cgcggggcgc gcgatgtggg
     3001 tctcgctgcg cgggtttgcc gaacgtttcg gcgccgccga atggttgggg gcggcggggc
     3061 gattgcgacc gagtcgcgag tttgggctgc cagcgttcga agcggacatc aaagcctggc
     3121 tcggcttcga cccactgcgt ctcctttcgc gcttgatgac tccgaagtag cagcaagcgt
     3181 ttctgcctca ctgaaggcga gcggcgaagg ggcttacccc gcgttacccg ctgccgttcc
     3241 tgaaggtgag acaatggcgt cgactccacc cgaaagagga ctgcgaggcg gtcgcgtcct
     3301 gttcgcggat gacgacccca gtgttcgcgc cgcgatcgcg cgcatcctca gggctcacgg
     3361 gtttttggtg gacctcgcct ccgatggaac cgaggcgctc gccttcgcgc gcgagtatcc
     3421 gtacgcgctc gtggc
//
LOCUS       DASTKS010000289         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1831331, whole genome shotgun sequence.
ACCESSION   DASTKS010000289 DASTKS010000000
VERSION     DASTKS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831331"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFQ35_06040"
     CDS             complement(<1..899)
                     /locus_tag="VFQ35_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9930223.1"
                     /translation="MTSRISEQNAFLVRVSDAVRGLNDPDAVARTACGLVAAHLGATE
                     SHWNEVDWEKRDFVVRDGFHNRDVQSVLTGRHAFASAGPAGAALLSGRSFLIEDARDD
                     ARLPSSMREALGERHMVSLLVVPVMVNGALRASLTVTQSAPRRWSTEEVALVEAVAAR
                     TWAEIERASAVSALEVELADTRELQRISSSLIERNDTGALYEEILEAARSLMRADFAS
                     IQMCAAERNELLLLAHHGFHPESARFWSRVSLDGKSSCALAMHRRELTIVPDVEICEE
                     FAGSDDLDEFRRSGIRAVQSTPLT"
     gene            1153..>2199
                     /locus_tag="VFQ35_06045"
     CDS             1153..>2199
                     /locus_tag="VFQ35_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9930224.1"
                     /translation="MRLDIEPDRDRERGTTRKRGAPLSATQIVVLTGGALAVVAVTVM
                     TAQVLLVIFGGALLGIVLFDSSRWLAEKLHIPRGVCLAVMVVVGLVLAAISAVLSAAF
                     LSEQIAALSKALPSLLRRAQAALEHVPIARNLSALLANPKLGEQAEKLPTTALAALSG
                     SLELMGAGALVFFVAVYGAAQPELHERALLSVVPQRHHTAVQKALERVRHTLSRWMLG
                     RLVAMLFVGLTSGIAFHLLHIPAAFALAVFAGLLTFVEYAGAIISAIPPLLLALAQGT
                     STAVAVLVVFTVLHVIEGYVLTPLLARVAVHFPPAFAVASQAIFGVLLGPLGLTFSTP
                     LLVAVSSAVQGARDS"
BASE COUNT          370 a          736 c          686 g          407 t
ORIGIN      
        1 gtgagcggtg tcgactgcac cgcccgaatc ccgctgcggc gaaactcgtc gagatcgtca
       61 gaccccgcaa actcttcgca gatctccacg tccgggacga tggtgagctc gcgtcggtgc
      121 atcgcgagag cgcacgagct cttgccgtcg agcgagacgc ggctccagaa cctcgcggac
      181 tcgggatgaa acccatggtg agcgagcagg agcagctcgt tcctttctgc ggcacacatc
      241 tgaatgctcg cgaagtccgc gcgcatgagc gagcgcgccg cctcgagaat ttcctcgtag
      301 agagcgccgg tatcgttcct ctcgatgaga ctgctactga tgcgttgcaa ctcacgagta
      361 tctgcgagct cgacctcgag agcgctgacc gcgctcgccc gctcgatctc cgcccacgtc
      421 cgcgctgcga ccgcctctac gagcgcaacc tcctcggtcg accaacggcg cggtgcggat
      481 tgagtcaccg tcagcgatgc gcgcaatgcg ccgttcacca tgaccgggac gaccagcaac
      541 gacaccatgt gacgctcgcc tagcgcctcc cgcatcgaag acggaagacg cgcatcgtcg
      601 cgcgcgtcct cgatgaggaa cgaccggcca gaaagaagtg ccgctcccgc tggtcccgcg
      661 gatgcaaagg catgccgccc agtgagcacg gactggacgt cccgattgtg gaagccatcg
      721 cgcacgacga agtcgcgctt ttcccagtcc acctcgttcc aatgggactc cgtcgctccc
      781 aaatgcgcgg cgactagacc gcacgccgtg cgcgcaaccg cgtcgggatc attcaagcca
      841 cgcaccgcgt cggaaacgcg gacgaggaaa gcgttctgct cactgatgcg agacgtcatt
      901 ccggctctcg ggctccggga gttcccggac agacaatgaa ctcgactatg acgcaagttg
      961 agcagaagaa gccagaacaa cgatgagacg acgcaatgct ctgcccccgc gcggcgcgtc
     1021 ttctcgaaag acaagaacgg tggacgaact tgcgagtgcg aggcgtcact gcatgacttc
     1081 gtgacgactg aacttgcatg aaatcccggt cgagctgcga gggcacgctc ttcgcaacgc
     1141 gcgctcgatc agatgcgact cgacatcgag cccgaccgcg accgggagcg agggacgacg
     1201 cggaaacgtg gggccccgct ctccgcgacg caaatcgtgg tgctgacggg cggcgcgctc
     1261 gctgtcgtcg ccgtcacggt catgactgcg caggtgctgc tagtgatctt cggcggcgcg
     1321 ctacttggaa tcgtgctttt cgacagctct cgctggctcg ccgaaaagct gcacataccc
     1381 cggggcgtgt gtctggcggt gatggtcgtc gtcgggctcg tcctcgccgc gatttccgca
     1441 gtgctgtccg ccgcattctt gagcgagcaa atcgcggcgc tgagcaaggc gctgccgagc
     1501 ttgttgaggc gcgctcaggc ggcgctcgag cacgtcccga tcgctcggaa cctgtccgcg
     1561 ttgttggcga acccgaagtt gggtgagcag gccgagaagt tgccgacgac cgcgctcgcc
     1621 gcgctctccg gctcgctcga gctgatgggg gcgggagcgc tcgtgttctt cgttgccgtg
     1681 tacggtgctg ctcagcccga gcttcacgag cgcgcgctgc tcagcgtcgt tccgcagcga
     1741 catcacaccg cggtccaaaa ggccttggag cgcgttcgcc acacgctcag tcgttggatg
     1801 ctcggcaggc tcgttgccat gctcttcgtc gggctgacga gcggcatcgc cttccatctt
     1861 ctgcacatcc cagccgcgtt tgccctcgcc gttttcgccg ggctgctcac cttcgtggag
     1921 tacgcgggcg cgatcatctc cgccatccca ccgctcctat tggcactcgc tcaaggaacg
     1981 agcacggccg ttgcggtcct ggtcgtcttt acggtgctcc atgtcatcga gggatacgtg
     2041 ctcactccgc tgctcgcgcg ggtcgcggtt catttcccgc cggctttcgc cgtagcgtcg
     2101 caggcgatct ttggagtgct gctgggtccg ctcgggctta cgttttccac accgctcctc
     2161 gtcgcggtat cctccgctgt gcagggcgcg cgcgatagc
//
LOCUS       DASTKS010000290         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1831367, whole genome shotgun sequence.
ACCESSION   DASTKS010000290 DASTKS010000000
VERSION     DASTKS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831367"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1252
                     /locus_tag="VFQ35_06050"
     CDS             320..1252
                     /locus_tag="VFQ35_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930225.1"
                     /translation="MNLTIESGSQGAYGRAVAASKRARWELERDVLRGRGLDVEHKFL
                     PDGLSLAHTLEFLTPSERRFMSQVQGRSYANIFGLVERFIAAKVLELSQQHCLGDQVA
                     LEALVRFSDEELKHQELFRRMEQLAAVGMPEGYEFSLDPNGVARAVLTKSTWSVLALT
                     CHIELFTLAHYKQSIEPDAGLSPLFKDVMMFHWKEESQHAVLDELEWRRVDARLTGTE
                     RATAVEDLLALVGAVDGMLQRQAAADADYFTKNSSRRLSDSEQARVRATFIRAYRYQY
                     IGSGIEQTRFPDILFGLVGEELAARIKVALGPLL"
     gene            1322..1735
                     /locus_tag="VFQ35_06055"
     CDS             1322..1735
                     /locus_tag="VFQ35_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET9930226.1"
                     /translation="MTLPLYTLVGFAAWTLLLLACTVGVHRWRLILTKRAAVNAFPAD
                     APDGPGWYQRATRAHLNCIENLPPYAAIVLTLTLAGRHGALIDALAIAVSCARVFQTL
                     IHVLFVQTERAVVFRFSFYTVQLLAMLWMVVLVFA"
     gene            1885..>2568
                     /locus_tag="VFQ35_06060"
     CDS             1885..>2568
                     /locus_tag="VFQ35_06060"
                     /inference="COORDINATES: protein motif:HMM:NF039329.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930227.1"
                     /translation="MRRLSLMACGVLAACCASNTEHGSGPPGVGELREGLRSTSSNNV
                     VPLTIAAGPRLGQAPLRTGVPFPMGALAPGSSFRLEDERGEPIPAQFDEIAHWPDGSI
                     ESVLVSFLADVGSERTLRLHYGCDARPRPSNSVRVTREGGKLVVNTGAVRAILDERGL
                     VTALTRRGAGARDSRPLLSMQDVFFENALDGKTYLASLADDAEAIVEEQGPVRATIRG
                     KGTLRGPGGR"
BASE COUNT          445 a          769 c          859 g          495 t
ORIGIN      
        1 gagggcgtcg ttctcgtcgc ccgacatcga gggcagcgtc tcgcgagatt tcatgagtgg
       61 gatcgcgaaa tatcatgaat tgctaaattt cgcgagcatg gtggcgctgg tatgtcgctg
      121 taaatataag caactattag attaacagca gtagatgcag tgtggcacgc cgttcgctct
      181 agccggactc atcgcgggca tcgcgcgctc gagaactggc gtgatgcggg aacgcgctcg
      241 gaagcgggcg agggcgctcg cgttcgcaga agaagccgaa accaacgctg tcacggcagc
      301 gagcacgaac ggaaaagcaa tgaatctaac catcgaatca gggagtcagg gtgcatatgg
      361 gcgtgcggtc gccgcgtcga agcgggcacg ctgggagctc gagcgtgacg tgttacgcgg
      421 gcgcgggctc gacgtcgaac acaagttttt gcccgatgga ctgtcgcttg cgcacacact
      481 cgaatttctg accccgagtg agcgtcgctt catgagccaa gttcaagggc ggagctacgc
      541 gaacatcttt ggtctcgtcg agcgtttcat cgcggcgaaa gtgctggagc tcagccagca
      601 acattgcttg ggcgatcagg tcgcgctcga ggctttggtt cgtttcagcg acgaagagct
      661 caagcaccag gagctattcc gtcgtatgga gcagctcgcc gcggtcggca tgccggaggg
      721 ctacgagttc tcgctcgatc cgaacggtgt ggcgcgcgcg gtcctcacca aatcgacctg
      781 gtccgttctg gcgctgacct gtcacatcga gctcttcacg ctcgcgcatt acaagcagag
      841 catcgagccc gatgcgggcc tttccccgct gttcaaggac gtgatgatgt ttcactggaa
      901 ggaagagagt cagcacgccg ttctcgacga actcgagtgg cgtcgagtcg acgcgcggct
      961 cacggggacc gagcgcgcga ccgcggtcga agacctcctc gcgctcgtgg gagcggtgga
     1021 cggaatgctg caacggcaag ccgccgcgga cgccgactat ttcacgaaga actcgagtcg
     1081 tcggctctcc gacagcgagc aagcgcgcgt gagagcgact ttcatccgcg cttatcggta
     1141 ccaatacatc ggttctggga tcgaacagac tcgttttccg gacattctct tcggactcgt
     1201 gggggaagag ctcgctgcgc gcatcaaggt cgcgctcggt ccgttgctgt gaaggggcgg
     1261 gggccgccag cgccggacgt cggcggcccc gctcgcgttg gcaacaacga aacgaagccc
     1321 catgaccttg ccactctata cactcgtcgg tttcgcggcg tggacgctgc tgctcttggc
     1381 ttgcacggtc ggcgtccatc gttggagact catcctgacg aagcgcgcgg ccgtcaacgc
     1441 cttccccgct gacgccccgg acggtccggg ctggtaccag cgggccacgc gcgcgcatct
     1501 gaattgcatc gagaacttgc ctccctacgc cgcgattgtg ctcacgctca ctctcgcggg
     1561 gcggcacggt gcgctcatcg acgcgctcgc aatcgcggta tcgtgcgcgc gcgtcttcca
     1621 aacgctgatt catgtgttgt tcgtgcagac cgagcgcgcg gtcgtgttcc gtttttcttt
     1681 ctatacggtg cagctcttag ccatgctgtg gatggtggtg ctcgttttcg catagcgccg
     1741 cgacgccgcc tcgcgcgaga accacgggtg cttgccgcct cgaggcaccg gtgatacctg
     1801 cgagtaacgc ccacctgggc gctgagtcgc tgatgagatt ccgtgccgac ccatcggaag
     1861 gcgcggccaa ccggagggat cgtgatgagg cgattgtcgt tgatggcgtg tggggtgctc
     1921 gctgcgtgtt gcgcttccaa tacggagcat ggcagcgggc cgccaggggt gggcgagctg
     1981 cgcgaggggc tacgttctac gtcgtccaac aacgtggtgc cgttgacgat tgcggcgggg
     2041 ccgcgcctcg gacaggcgcc gctccgtacc ggcgtccctt ttcccatggg cgcgcttgct
     2101 cccggcagca gctttcggct ggaggacgag cgcggcgagc ccattcccgc tcagttcgac
     2161 gaaatcgctc actggcccga tgggtcgatc gagtcggtgc tggtcagctt cttggcagac
     2221 gtcggcagcg aacgcacgct gcgactgcac tacggatgtg atgcgaggcc acggccgagc
     2281 aattcggtgc gggtgacgcg cgaagggggg aagctcgtcg tcaacaccgg agcggtgcgc
     2341 gcgatcctgg acgagcgcgg gctcgtgacc gcgctgacgc gaaggggggc tggagcccgt
     2401 gattcgcgtc cgctcctttc gatgcaagac gtctttttcg agaacgcttt ggacggcaag
     2461 acttaccttg cttccctcgc cgatgatgct gaagccatcg tcgaggagca gggccccgtg
     2521 cgcgctacca tccgcggcaa ggggacgctg cgaggtccgg gcgggcgt
//
LOCUS       DASTKS010000291         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_938199, whole genome shotgun sequence.
ACCESSION   DASTKS010000291 DASTKS010000000
VERSION     DASTKS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938199"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFQ35_06065"
     CDS             <1..446
                     /locus_tag="VFQ35_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9930228.1"
                     /translation="AHDGAPVRSARIVVIADGATLAEVVTDESGEFEISLPGVDTRGA
                     LIRIESTWHSRLEKPLPPPGQLLISLLTRRRALLAALVDWAERRGRPFVGDREPTPRE
                     VREIATQSAEPAVATWARGVEDAAFGPNPVDADREAGVRAQEPKP"
     gene            617..1891
                     /gene="ftsY"
                     /locus_tag="VFQ35_06070"
     CDS             617..1891
                     /gene="ftsY"
                     /locus_tag="VFQ35_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9930229.1"
                     /translation="MELVYVLIALVLIVGAYFLFFQKSAPEKIEPRPSQKPLAEKRKE
                     SRPPQEAKPSKPPSEAKPAKAEPDKADVPEVEPDEAPPPRHTSTPPPSPVRAKPSDHP
                     PRASAPPAPSALEKLRRGLSRSRQTEGFFGRLRALFRGKTDISPELAREIEEVLLTSD
                     VGVETTKALLERVQAELASSGGQADPERVWDALAAEARTLLEIGGLGGTVELRAKPTV
                     ILMVGVNGAGKTTTIGKLATKFHAAGHKCVLAAGDTFRAAAVQQLVVWGERVGCEVVR
                     GKDGADPGSVVFEAIKRAQESGADLVLADTAGRLHTKSNLMQEMKKIAKTVEKALPGA
                     PHEIFLVIDATNGQNAIAQAREFREALPVTGIVLTKLDGTAKGGVVLGIANTLKLPVR
                     FIGLGERPDDLHEFRPAEFVEALLGREAGAAA"
     gene            2069..3088
                     /locus_tag="VFQ35_06075"
     CDS             2069..3088
                     /locus_tag="VFQ35_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HET9930230.1"
                     /translation="MKNCRVGLLVAAALGILPATSWAQGKGAKGAAPASGDAASGEAE
                     AAPAEGEAGDKAAGEAAPAEDAAGGDETPEGGGLAEICKIDPAACPNLDMEKEAARPL
                     KESIYAMQQIFALRARRFEVNPYFGITFNDQFVSHPGPGLALNYYITNVLAVGINYNY
                     FGAFNVDRKLNAQVRRSARVAVPLTEYNMGGNLNFTYVPAYGKFAGFGDFIFHYDAYV
                     VGGVGAIQTRPIPVIDPDNRNFEYQWKIAFNAGIGLRIFFNRWLGATLEMRDYIFPDK
                     LESLSNTLQAGQTSPADANYWYDDGKGGGTSYSKAKARLTNAVQAQLGLSFFLPFSFE
                     YRLPK"
     gene            3102..3983
                     /locus_tag="VFQ35_06080"
     CDS             3102..3983
                     /locus_tag="VFQ35_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930231.1"
                     /translation="MNSSKGKTAKRVLAALAATFALCAFTQRAEAQEILLTGPLAGAP
                     AVRKLRLHRQTRFEIAPLASFTLLDPYERTILFGGRLNFNFTDWLGVGVYGAFGAVKI
                     PTALTDHIQEVNTQRRNEAAAEGTDYLTTTTGRLTRVNLGPQFKNQIGEIDWIFAPQV
                     TLVPFRGKIALFQSIFVDTDLFFFGGPAFVGVKERKNCEGPACDAYDRASRVAIAPTF
                     GLGLSFYVNKWSAFGLEWRGLPFSRNLGGFDTAGGGKDDKFPDNKISSADQRFEFNQM
                     LTVSYSFYLPTQYKISE"
     gene            complement(4248..4985)
                     /locus_tag="VFQ35_06085"
     CDS             complement(4248..4985)
                     /locus_tag="VFQ35_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9930232.1"
                     /translation="MGLFDIFSKKKADAPRLNERDLARYERLVGSKLSQNIDRQEAID
                     TLARMGTAGAARVLLKRFDWALDPSITDQEEKETCVRGIVAAGDLALEPVRAYCKKAE
                     SLTWPLRVLKSIVPPESYSEELLALLDQYDTEYVRNTEPKVQLIAELGQFKSEDVRVA
                     LEPFLQDASEPVRFATVTSVFELGFEASTPALIAALAGEESQRIKNRIAQRLAELGWS
                     VPSELKEAAQSALPSGYGIQGQRVVAA"
BASE COUNT         1012 a         1715 c         1702 g          958 t
ORIGIN      
        1 atgcccacga cggcgcgccc gttcgcagcg ctcgcatcgt ggtgatcgcg gatggggcga
       61 cgctcgccga ggtggtcacc gacgaaagcg gagagttcga gatctcactt ccgggcgtcg
      121 acacccgcgg tgctctcatc cggatcgaat cgacttggca ttcgcgcctc gaaaagcccc
      181 tccccccgcc cggccagctc ttgatctcgc tcttgacgcg aaggcgcgcg cttttggcgg
      241 cgctcgtgga ttgggcggaa agaaggggcc gcccgttcgt cggcgaccgc gagccgaccc
      301 cccgcgaagt gcgagagatc gcgactcaaa gtgcggagcc tgcggtggcg acgtgggcgc
      361 gaggggtcga agacgcagct ttcgggccaa acccggtcga tgccgaccgc gaagccggcg
      421 tgcgcgcgca agagccgaaa ccctgatgtt tcgtgtttcg cggcgaaaat ggcgacctgc
      481 cgcgggaggt agtctcgcgc tgagcgcagc tgcgcgggca ctctcgtgcc tccgggtatg
      541 ataacgaccg cacgcggagg ttgacgggcc ttcccgctgg ttcctatagt cgcgcgccca
      601 acctcgcgct gactcgatgg agctcgttta cgttctcatc gcgctcgtac taatcgtcgg
      661 cgcttatttc ttgttctttc aaaagagcgc gcccgaaaag atcgagccgc gcccttcgca
      721 gaagcccctt gccgaaaagc gcaaggagtc acgtccgccg caagaggcga agccgagcaa
      781 gccgccgagc gaggcgaagc cggcaaaggc cgaaccggac aaagccgacg tgccggaggt
      841 cgagcccgac gaggctcccc cgccgcggca caccagcacg ccacctccgt cgccggtgcg
      901 cgccaagccg agcgatcatc cgccgcgcgc ctccgccccg cctgcgccga gcgctctcga
      961 gaaactgcgg cgcggcctat cgcgctctcg tcagaccgaa gggttcttcg gccgactgcg
     1021 cgcgctgttt cgcggcaaga cggacattag cccggagctc gctcgcgaga ttgaagaggt
     1081 gttgctcacc tccgacgtcg gcgtcgagac aacgaaggcg ctgctcgagc gcgtgcaagc
     1141 cgaactcgcg tcgagcggtg ggcaagccga tccagaacgc gtttgggacg cgcttgctgc
     1201 cgaagcacga acattgctgg aaataggtgg tctcggcggc acggtcgagc tccgcgcgaa
     1261 gcccaccgtg attctcatgg tgggagtcaa cggcgccggt aagacgacca ccatcggtaa
     1321 gcttgctacc aagttccatg ccgcgggtca caagtgtgtt cttgctgctg gtgacacttt
     1381 ccgtgcagca gcagtgcagc aactcgtggt atggggagaa cgagtcggtt gcgaagtcgt
     1441 gcgcggcaag gacggagcag atcctggcag cgtcgtcttc gaagccatca aacgagcgca
     1501 agaaagcggt gcggatttgg ttctcgccga caccgccggt cgactgcata cgaagtccaa
     1561 cttgatgcag gaaatgaaga agattgccaa gacggtcgaa aaggcgctcc ccggagctcc
     1621 ccacgagatt ttcttggtga tcgacgcgac caacggacag aatgcaatcg ctcaagcgcg
     1681 cgagtttcgc gaagcactac ccgtcacggg aatcgtgctc acgaagttgg acggcacagc
     1741 caaaggcggt gtcgtgctgg gaattgcgaa cactttgaag ttgcccgttc gcttcatcgg
     1801 tctcggtgag cggccagacg atctgcacga gtttagacct gcagaattcg tcgaagcgct
     1861 cctcgggcgt gaggccggag cagcagcgtg acgcgggttc ataaacccgc gaaattaagc
     1921 ctagactcta aatttttgct tgttgatccc tggaatttca gcgtgatata gtgcgccggc
     1981 gttttcggac ggcggattta tttaatagtt ccatgcattt gcggactatg tgcagcggtc
     2041 gtcgcagggg caaccgaaag aggcatcgat gaagaactgt cgcgtcgggc tcctcgtagc
     2101 agcggccctc ggcatcctcc ccgccaccag ttgggcgcag ggtaagggtg ccaagggagc
     2161 agcgcctgct tcgggagatg ctgcgagtgg agaggcggag gccgcccccg cggaaggtga
     2221 agcaggagac aaggcagccg gtgaagcagc gccggcagag gatgccgctg gcggagacga
     2281 aactccggaa ggcggcggtc tcgcagaaat ctgcaagatc gatcctgccg catgtcccaa
     2341 cctggacatg gaaaaggagg cagcgcgccc gctgaaggaa agcatctacg cgatgcagca
     2401 gatcttcgcg ttgcgcgcgc gccggttcga ggtgaatccg tatttcggaa tcactttcaa
     2461 cgaccagttc gtgagccacc cgggccccgg cttggcactg aactactaca tcacgaacgt
     2521 gctcgcggtc ggcatcaact acaactactt cggcgcgttc aacgtcgacc gtaagttgaa
     2581 tgctcaggtg cgccgctcgg cgcgcgtcgc ggtgcccctc accgagtaca acatgggggg
     2641 taacctgaac ttcacgtacg tgcccgcgta cggcaagttc gccgggttcg gcgacttcat
     2701 cttccactac gacgcgtacg tggtcggtgg cgtcggcgcg attcagacgc gccccatccc
     2761 ggtcatcgac cccgacaacc gcaacttcga ataccaatgg aagatcgcgt tcaacgccgg
     2821 catcggcctg cgcatcttct tcaatcggtg gctcggcgcc acactcgaga tgcgcgacta
     2881 catcttcccg gacaagctcg agtcgctctc gaacacgctg caagcggggc agacgtcacc
     2941 ggctgacgcc aactactggt acgacgacgg caagggtggc ggcacgagct acagcaaggc
     3001 caaggcgcgc ttgaccaacg ccgtgcaagc acagctcggc ttgtcgttct tcctcccgtt
     3061 ctcgttcgag taccgactgc cgaagtgagc aagggcgaag catgaactcc tccaaaggca
     3121 agacagcaaa gcgggttctg gcggctctgg cagccacctt cgccctctgc gcgttcaccc
     3181 agcgcgccga agcacaagag attctgctga ccggtcccct cgcgggcgca ccggcggtgc
     3241 gaaagctcag gcttcatcga cagacgcgct tcgaaatcgc tcccctcgcg tcgttcaccc
     3301 tcctcgaccc gtacgagcga acgattctgt tcggcggtcg tctgaacttc aacttcacgg
     3361 actggctcgg cgtcggtgtc tacggtgcct tcggcgccgt aaagatcccg accgcgctca
     3421 ccgaccacat ccaagaggtg aacacccagc gccggaacga agcagccgcg gaagggaccg
     3481 actaccttac gacgaccacc ggccgactca ctcgcgtcaa cctgggtccc cagttcaaaa
     3541 accagatcgg cgaaatcgac tggatcttcg cgccgcaggt cacgctcgtt ccgttccgcg
     3601 gcaagatcgc cctgttccag agcatcttcg tcgacaccga cctgttcttc ttcggcggcc
     3661 cggctttcgt gggcgtcaaa gagcggaaga actgcgaagg ccctgcttgc gatgcttacg
     3721 atcgcgcgag ccgcgtcgcg atcgctccga cgttcggcct cggcctctct ttctacgtga
     3781 acaagtggag cgcgttcggt ctcgagtggc gcggcctccc cttctcgcgc aacttgggcg
     3841 gcttcgacac cgcgggcggc ggcaaagacg acaagttccc cgacaacaag atctcgagcg
     3901 ccgaccagcg cttcgagttc aaccagatgt tgacggtgag ctacagcttc tacctgccga
     3961 cccagtacaa gatcagcgag tagcgaagcg ctgtcgcaga atcgcgaatc gggcgagtct
     4021 caatggctcg cccgattgct tttgtaccga gctggtggct ccgctcggat catcgcgaaa
     4081 cgagatcgcg gcgccttcga ccgcgatggt gcgcctggtt tggcccaagc ctttctcgcc
     4141 gcccggcgct gctccaagcc gcgccttact ggaagcgcga cccctcgcct aggcggacct
     4201 gtgctcccaa cgcagttcgg agcagcgggc ccgggccagg cgccagctta ggccgcaacg
     4261 acgcgctggc cctgaatgcc gtagccgctc gggagcgcgc tttgagccgc ttccttgagc
     4321 tcggacggca ccgaccaacc gagctcggcg aggcgctgcg cgatgcggtt cttgatgcgt
     4381 tgcgactcct caccggcgag agcagcgatg agcgcggggg tgctcgcttc gaagccgagc
     4441 tcgaagactg acgtcacggt ggcgaagcgc acgggttcgc tcgcgtcctg caagaagggc
     4501 tcgagcgcga cgcgcacgtc ttcgctcttg aattgcccga gctcggcgat gagctgcacc
     4561 ttgggctccg tgttgcgaac gtactcggtg tcgtattggt cgagcagcgc gagcaactcc
     4621 tcggagtagg actcgggagg gacgatgctt ttcaagacgc gcagcggcca ggtgaggctc
     4681 tccgccttct tgcaataggc gcgcactggc tcgagcgcga gatcgccagc ggcgacgatg
     4741 ccgcgcacgc acgtctcttt ttcttcctga tccgtgatgg aggggtcgag cgcccaatcg
     4801 aagcgcttca acaagacccg cgccgcacca gcggtaccca tgcgcgccag cgtatcgatc
     4861 gcttcttggc gatcgatgtt ttgcgacagc ttgctgccga ccaagcgctc gtagcgagct
     4921 aggtcgcgct cgttgaggcg tggcgcgtcg gccttcttct tggaaaaaat atcgaaaagt
     4981 cccatgggtc gttgccgtcg agccgctcat atagcgcgcc tcgacgcttt ctacaggtct
     5041 cattcatgcg cccgcagcgc ataactgatt tgggcgtggc gtttggggtt ctacggcccc
     5101 aagccatcct accgaagacg tgagcggtcg gaagctaccc gagtgattgg gagccacgcg
     5161 ggcgcaaatc ggactgccac cgaggagcgg gcgcagcgac gtgcaccgcc gacgagggtt
     5221 cgtttggaac tcgttcgggc accagccccg cctcgatgac acagggctgc gctgggctgc
     5281 ctcgtgcgtg cttgtcgcga cgttgccgag ggccctgacg tttgccgagg gccctgacgt
     5341 ttgccgaggg ccctgacgtt tgccgagggc cctgacgttg ccgaggg
//
LOCUS       DASTKS010000292         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_493317, whole genome shotgun sequence.
ACCESSION   DASTKS010000292 DASTKS010000000
VERSION     DASTKS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493317"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..525
                     /locus_tag="VFQ35_06090"
     CDS             106..525
                     /locus_tag="VFQ35_06090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET9930233.1"
                     /translation="MAETIFSKILRNEIPCHKVYEDAHVLAFLDINPLSRGHVLVIPK
                     EPAETLDALSDEASAAIGRVLPRLCRAVKRVSGASSYNILQNNGAPAHQAVFHVHFHI
                     IPKPNETEGLGIRWPSGKLPNDQGAALATELRAALGS"
     gene            complement(782..2419)
                     /gene="pgi"
                     /locus_tag="VFQ35_06095"
     CDS             complement(782..2419)
                     /gene="pgi"
                     /locus_tag="VFQ35_06095"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9930234.1"
                     /translation="MTSPRSDLSSLPAWQALGQHKKSIEAQSLRELFQSDPQRGERLS
                     LEASGVYLDYSKNRVTDETLKLLVSLAEQSGLREHIDAMFRGEKINSTEGRAVLHVAL
                     RAPKTASILVDGKNVIPDVHAVLDKMADFSERVRSGAFKGHTGRRLKNVVNIGIGGSD
                     LGPVMAYEALRHYSERSLRMLYVSNIDGTDMAEALRDLDAEETLFIVCSKTFTTLETL
                     VNARSARSWLVERLGTDAAVSKHFVAVSTNAAEVSKFGIDTANMFGFWDWVGGRYSYT
                     SAIGLSLMLAIGPVRYREMLGGFHEMDEHFRTAPFAKNLPVLLGLLGVWYNNFFGAES
                     HAVLPYDQYLARFPAYLQQLDMESNGKSVTIDGKPVTYETGPIIWGQPGTNGQHAFYQ
                     LIHQGTKLIPCDFIGFIRTLNPLPHHHDNFMSNLFAQTEALAFGKTAEEVASEGTRAE
                     LVPHKVFTGNRPTNTMLLDELTPAALGRLVALYEHKVFVQGSIWHINSFDQWGVELGK
                     VLAKKVAAELESTSDPALAHDSSTNALIRRYRARRGR"
     gene            complement(2502..3227)
                     /locus_tag="VFQ35_06100"
     CDS             complement(2502..3227)
                     /locus_tag="VFQ35_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9930235.1"
                     /translation="MRLKVVSWNIHKGIGGVDRRYALDRVIGVLVDQKPDIVLLQEVA
                     EGLPRAKNDNQVELLSEALEMPHVAYGREHRFRVGGYGNLIMSRFHLTDVHHIDLTIR
                     WRKKRGVICARTHVHYDGHQRSVVIFNLHLGLAGSERGEQLEKFLDCHPFKGLHHHTP
                     IVLGGDLNDVWGSLGPRFLAPAGFQRAGNLYNTFPAMLPLRPLDGIFVRGDLRAAHCG
                     PRRSELARSASDHLPLVAELDLV"
BASE COUNT          620 a         1061 c         1182 g          617 t
ORIGIN      
        1 catcgcgcct ccgtcgagtc gctgcgaatt gccgagaacc gagctcaatt tcagcatggt
       61 tcagtcccat ccgtcgcccc cgcgggcacg gtgctaaaag gggctatggc cgagacgatc
      121 ttcagcaaga tcttgcgcaa cgaaattccc tgccacaagg tctacgagga cgcacacgtc
      181 ctcgcgtttt tggacatcaa cccgctcagc cggggccacg tgctcgtcat ccccaaggag
      241 ccggcagaga cactcgatgc tctctctgac gaggcttcgg ctgccatcgg gcgggtcttg
      301 ccgcggctct gtcgcgccgt gaagcgcgtt tcgggcgcga gctcctacaa catcttgcaa
      361 aataacgggg cgccggcgca ccaagccgtc ttccacgtac actttcatat catccccaaa
      421 ccaaacgaga ccgaaggact cggcatccgc tggccctccg gcaagctccc caacgaccag
      481 ggcgcagcgc tcgctaccga gcttcgcgca gcgctcggga gctgaggcgc caacgcgcat
      541 cgagcttcgc gcagcgctcg ggagctgagc gccaacgcgc atcgagcttc gcgcagcgct
      601 cgggagctga gcaccgacgc gcatcgagct tcgcgaagcg cgctcggagc tgaggcgcca
      661 atgcgcatcg agcttcgcga agcgccgccg aaggcacctc gtatgcaagt cggcggcaat
      721 ccggggcagg cagtgggccc ggtgcgcgct cagcgtcgcg caccgggggc caaccaaata
      781 atcagcgacc gcgacgcgct cgataacgac ggatgagcgc gttcgtggag ctgtcgtgcg
      841 caagcgccgg atcgctcgtc gactcgagtt cggccgcgac ctttttggcc agcactttgc
      901 cgagttcgac gccccactgg tcgaaggagt tgatgtgcca aatgctgcct tgcacgaaga
      961 ccttgtgctc gtagagcgcg acgagtcggc caagcgcggc gggcgtgagc tcgtcgagta
     1021 gcatcgtgtt ggtggggcgg ttaccggtga agaccttgtg ggggacgagt tcggcacgag
     1081 tgccttcgct cgcaacttct tccgccgttt tgccgaaggc gagcgcttcg gtttgggcga
     1141 agaggttcga catgaagttg tcgtggtgat ggggcagcgg gttcagcgtg cggatgaagc
     1201 cgatgaagtc gcaagggatg agcttcgtgc cctgatggat gagttggtag aaggcgtgct
     1261 ggccgttcgt gcccggctgg ccccagatga ttgggccggt ttcgtaggtg accggcttgc
     1321 catcgatggt gacgctcttg ccgttgctct ccatgtcgag ctgttggagg taggcgggga
     1381 agcgtgcgag gtactggtcg tagggcagca ccgcgtggga ctcggcgccg aagaagttat
     1441 tgtaccaaac gccgagcaag cccaagagca cgggcaggtt cttggcgaaa ggcgcggtgc
     1501 ggaagtgctc gtccatttcg tggaagccgc cgagcatttc gcgatagcga acgggcccga
     1561 tggcgagcat caaggagagg ccgatcgcgg aggtgtaaga gtagcgcccc ccgacccaat
     1621 cccagaagcc gaacatgttg gcggtgtcga tgccgaactt cgagacctcg gcggcgttgg
     1681 tgctgaccgc gacgaagtgc ttgctcaccg ctgcgtcggt gccgagtcgc tccacgagcc
     1741 agctccgcgc cgagcgtgcg ttgacgagcg tctcgagcgt cgtgaacgtt ttcgagcaaa
     1801 cgatgaagag cgtctcttcg gcgtcgagat cgcgcagcgc ctcggccatg tcggtgccgt
     1861 cgatgttcga cacgtagagc atccgcaggc tgcgctcgct gtagtgacgg agcgcttcgt
     1921 acgccatcac ggggccgaga tccgagccgc cgatgccgat gttgacgacg ttcttgaggc
     1981 gcctgccggt gtggcccttg aaggcgccgg agcgcacgcg ctcactgaag tcggccatct
     2041 tgtcgagcac ggcgtgcacg tcggggatga cgttcttgcc gtcgacgagg atgctcgccg
     2101 tcttcggcgc acggagcgcg acgtgcagta cggctctgcc ctcggtgctg ttgatcttct
     2161 cgccgcggaa catcgcgtcg atgtgctcgc gcaagccgct ctgttcggcg agcgacacga
     2221 ggagcttcaa cgtctcgtcg gtgacgcggt tcttcgagta gtcgaggtag acaccggaag
     2281 cctcgagtga aaggcgctcg ccgcgttgag gatccgactg gaagagctcg cgtaagctct
     2341 gcgcttcgat gctcttcttg tgctgaccga gagcttgcca ggcggggagg gacgaaaggt
     2401 cggaacgagg cgacgtcatg cgcgtaggta aaccgccgcg ccgtccggcc gtcaaccgcg
     2461 ctcgtcgcgg gctcgccgag cgctcggcga cgcggagccg gctaaacgag atccaactcg
     2521 gccacgagcg gcagatggtc cgaggcggag cgcgccagct cgctgcgtcg cgggccgcag
     2581 tgggccgccc tcaagtcgcc gcgcacgaaa atgccgtcca agggacgaag cggcagcatc
     2641 gcggggaacg tgttgtacag gtttccggcg cgctggaagc cggccggagc caggaagcgc
     2701 ggcccgagcg agccccacac gtcgttcaag tcaccgccga gcacgatggg cgtgtggtgg
     2761 tgcaagccct tgaaggggtg gcaatcgagg aacttctcga gttgctcgcc gcgctcggag
     2821 ccagcgagcc cgaggtgcaa gttgaagatg acgacgctgc gctgatggcc gtcatagtgg
     2881 acgtgcgtgc gcgcacagat gacgccgcgc tttttgcgcc aacgaatggt gagatcgatg
     2941 tggtgcacat cggtcaggtg aaagcggctc atgatcaggt tgccgtagcc gccgacccga
     3001 aagcggtgct cgcgtccata agcgacgtgc ggcatctcga gagcctcgct caggagctcc
     3061 acctggttgt cgttcttcgc gcggggcagc ccctcggcta cttcttgaag caacacgatg
     3121 tcgggcttct ggtccacgag caccccgatc acccgatcga gtgcgtagcg ccggtcgacg
     3181 ccaccgatgc ctttgtggat gttccagcta acgaccttga gccgcatcca gcgcggagta
     3241 tggcacaatt cggctcgagc tcgccgctcg agtcctggtt tcgatcggcc caggatggcg
     3301 aagtttttcc ggcgaggccg gccgactgtc acgtgcgcgg ccggcgggta tgcacggaga
     3361 ggccgacctg cgatagactc gtgcgcgacg cctcgaaatg ggccttcgaa gaagcgaatg
     3421 aacgattcgt ccacgctcga ctacgcgggc aagatcatcg ccggacgtta ccgggtcgag
//
LOCUS       DASTKS010000293         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_47672,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000293 DASTKS010000000
VERSION     DASTKS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47672"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFQ35_06105"
     CDS             complement(<1..586)
                     /locus_tag="VFQ35_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930236.1"
                     /translation="MRRGAACALFALLVGCGAHRQAQQAQAVQAANPQAVNRMAEGVE
                     AAKQADGKPRAIGLFEQAVRADASLWEARYDLGVLYADAGNLAAAERELAESAQLSPN
                     SEDVVLALAEVRRRRRDPASAIQALESFVKNNPQATVAPIALMTVLREGGKVDAAIDL
                     AHRLLVRRAHDPYVLSELALSHLAKDEVDTAELLI"
     gene            complement(586..>2648)
                     /locus_tag="VFQ35_06110"
     CDS             complement(586..>2648)
                     /locus_tag="VFQ35_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930237.1"
                     /translation="YKWIVREYVAPPKAGAGKVSAWVSVQKPETLAEAETAIEKQMRE
                     DAVGLHAKAQSDKTSRGEFEGAAHLYEVYLSRFSNRPEAFEMYYNLAEIYFYRLEDAN
                     KAADAYLAAVKLNPKAELSRTALYNALSALEVSRASEFEAAKKAGKKQEETATDQKLT
                     EAMELYVKNYPNDPQIPELLFRQGKLYYDYEVFDPAVRQWGLLLEKYPTSKYSAGAGE
                     LILDSFNKSKDYANIETWARRLKTAPAFQSPAQQARLDTLIIGAVFKLGEQLSQKGEH
                     EKAAEAYLRAAREFPKEPRAAQAAVNAEVEAKRAGNLAMLSGAADILWKQHRQRPEAA
                     EGLWIAATTHQEVGLFSEAAGYHALIAEAFPKFEHHRDAAFNAVLLRVTVGEHDKAIE
                     SGEKFKKYYPRDESLDEVAFLMGKAHEKAGKKQEAALVYDRYAKNARSASSQIEALVR
                     LAIVSNDEKTRAASLDRAVHIYAARKATLDDRGKYYAARARYLEGAAFLEKFDAVKIE
                     GDVQKLKERLKKKSELLKKAADAFLSTAEMGVAEWTTASLYQIGFTYESFSKALLNSP
                     PPASLSEAEKELYRQSIEEFVIPIEERSLEAYESGWQKARELGIFNSWTAKMREALGR
                     LNAELYPPLKETGFELRSRGPQALPALIEAPRRAQTGAYLIQPSSEKKPAANGKKQRK
                     EAQP"
BASE COUNT          406 a          877 c          824 g          541 t
ORIGIN      
        1 ggatcaggag ctcagccgta tcgacctcgt ccttggcgag gtgagacagc gcgagctcgg
       61 agagaacgta gggatcgtga gcgcgccgga cgagcaagcg atgcgcgaga tcgatcgcgg
      121 cgtcgacctt tccgccctcg cgcaacaccg tcatgagcgc gatgggagcg acggtcgcct
      181 gcgggttgtt cttcacgaac gattcgagtg cctgaattgc cgacgcagga tcccttcggc
      241 ggcggcgaac ctcggccaaa gcgagcacga cgtcctccga gttgggagac agctgagccg
      301 actccgcgag ctcgcgctca gcggcggcga ggttgcccgc gtccgcgtac agcacgccga
      361 ggtcgtagcg cgcctcccac aacgaagcgt cggcccgcac cgcttgctcg aacagtccga
      421 tcgcgcgcgg cttgccgtcg gcttgcttag ccgcttcgac gccctcggcc atgcggttca
      481 ccgcttgagg gttcgcggcc tgtaccgctt gcgcttgctg agcctggcga tgagcaccgc
      541 atcccacgag cagagcgaag agcgcgcatg cagcgccacg gcgcatcacg gctgtgcctc
      601 cttgcgttgc ttcttgccgt tggccgcggg cttcttttcg gagctcggtt ggatcaaata
      661 cgcgccggtc tgagcgcgcc gcggcgcctc gatcaacgcc ggcaacgcct gcggaccgcg
      721 tgaacgaagc tcgaacccag tctctttcag gggtggatag agctcggcgt tcagacgccc
      781 caacgcctcg cgcatcttcg cggtccagct attgaagatt ccgagctcgc gcgccttttg
      841 ccagccgctc tcgtacgctt cgaggctgcg ctcttcgatc gggatcacga actcttcgat
      901 cgattggcgg tagagctcct tttcggcctc gctcaagctg gcgggggggg gagagttcaa
      961 gagcgccttg gagaacgact cgtacgtaaa gccgatctgg tagagcgaag ccgtcgtcca
     1021 ctcggccacg cccatctccg ccgtcgagag gaacgcgtcc gcggccttct tcaagagctc
     1081 gctcttcttc ttcaaacgct ctttcagctt ttgcacgtcg ccctcgatct tcaccgcgtc
     1141 gaatttctcg aggaacgctg caccttccag gtagcgggcg cgcgcagcgt agtacttgcc
     1201 gcgatcgtcg agcgtggcct tgcgcgcggc gtagatgtgc acggcgcgat cgagcgaagc
     1261 cgccctcgtc ttttcgtcgt tgctgacgat cgcgaggcgc acgagggctt cgatttggct
     1321 gctcgcgctg cgggcgttct tcgcgtagcg atcgtacacg agcgcggcct cctgcttctt
     1381 gccggctttc tcgtgcgcct tgcccatcaa gaaggcgacc tcgtcgagcg actcgtcgcg
     1441 cgggtagtac ttcttgaact tctcaccgct ttcgatggcc ttgtcgtgct cgccaaccgt
     1501 cacgcgcagc agcacggcgt tgaacgcggc gtcgcggtgg tgttcgaact tgggaaaagc
     1561 ttcggcgatg agagcgtggt aaccggccgc ctcgctgaac aggccgacct cctgatgagt
     1621 ggtcgccgcg atccacaaac cttccgccgc ctctgggcgt tgccggtgtt gcttccagag
     1681 gatgtcggcg gcaccggaca gcatcgccaa gttgccggct cgcttggcct ccacctcggc
     1741 gttgaccgcg gcctgggccg cgcgcggctc tttcgggaat tcgcgcgccg cgcgcaggta
     1801 agcctccgct gccttctcgt gctcgccctt ttgactcagc tgctcaccga gcttgaacac
     1861 ggcaccgatg atcaaggtgt cgagccgcgc ttgctgagcg ggcgattgga aagcgggcgc
     1921 cgtcttcaaa cgacgcgccc acgtctcgat gttcgcgtag tccttgctct tgttgaacga
     1981 gtcgaggatg agctcaccgg cgccagccga gtacttgctc gtcgggtact tctcgagcaa
     2041 aaggccccac tgccgcacgg ctggatcgaa gacctcgtag tcgtaataga gcttgccctg
     2101 ccggaacaag agctccggga tttgcgggtc gttcgggtag ttcttgacgt agagctccat
     2161 cgcctcggtc agcttctgat ccgtggccgt ctcctcctgc ttcttgccgg ccttcttcgc
     2221 ggcttcgaac tcggacgctc gcgacacctc gagcgcggag agcgcgttgt agagcgcggt
     2281 gcgcgacagc tccgccttcg ggttcagctt caccgccgcg aggtacgcgt cggcagcctt
     2341 gttcgcatcc tcgagccgat agaagtaaat ttcggcgagg ttatagtaca tctcgaaggc
     2401 ctctggccgg ttcgagaagc gcgacaggta gacctcgtac aggtgcgcgg ccccctcgaa
     2461 ctcgccgcga ctcgtcttgt cgctctgcgc cttcgcgtgg agcccaaccg cgtcttcacg
     2521 catctgcttc tcgatcgccg tctctgcctc ggccagtgtc tccggctttt gcaccgagac
     2581 ccatgccgag accttaccgg cgcctgcctt cggcggagcg acgtactcgc gcacgatcca
     2641 cttgtagt
//
LOCUS       DASTKS010000294         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_716100, whole genome shotgun sequence.
ACCESSION   DASTKS010000294 DASTKS010000000
VERSION     DASTKS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716100"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFQ35_06115"
     CDS             complement(<1..231)
                     /locus_tag="VFQ35_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9930238.1"
                     /translation="MPADRTLPIMRTPPAAFDELPDGVLVLDAEGRIETANRAFSELV
                     QREQEELVGKRIEAITAEEDILRILGFEAVFGG"
     gene            complement(221..1366)
                     /locus_tag="VFQ35_06120"
     CDS             complement(221..1366)
                     /locus_tag="VFQ35_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9930239.1"
                     /translation="MTEANLPRILCVDDEQRVLEGLERTLFDHFEVVTLSKPEQALEL
                     LKETEEPFAVLVSDMRMPGMDGATLLSHARRIAPDTTRVLLTGHAELDAAVAAVNQGN
                     IFRFLCKPCAPEVLLPALTEAAGQHRLLVSERELLGETLTGSIRLLTEILSMIAPQVF
                     SRTQRVRMLVMHMVERLGHEQRWKFELAAALSLIGCVGVPEETLTRAIAGLPLEPEEK
                     QAFAEHPLIAYRLLAPIPRFQEVAEMVRRQMRVNEPDPTPDVEIGAAMLRIARDVEAL
                     VETGLTVEESVKKLAPKHNERERSLAALLRDFTGMSPKTVNVRVLRVTQLTGEMTLED
                     DVRTTTGVVVVPKGRQLNMVLLERLLKFSRAGSLIEPIRVQVPRHAG"
     gene            complement(1363..2517)
                     /locus_tag="VFQ35_06125"
     CDS             complement(1363..2517)
                     /locus_tag="VFQ35_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930240.1"
                     /translation="MRVLFVDDEPRVLEAIERLFFDLDVDWETCFASTIDGALEAFRS
                     APVDVIVCDMRMPGQDSATLLNRVCELDPRTIRIVLSGQTDEASALRVVPVAHQFLAK
                     PCDGATLRRVIENTRELRQLLDDTKLQSLVGQVGHLPSPPRLFSEISRLLEDESADAT
                     RVADLVQQDPAMATKILQVANSALFASSHAIADVKTAVVRLGTRTIRNLALGVGAFEA
                     ARGSAKALSGFIDELQNRSLSIGRLARALSPKSVDADAAFMAGLVCDLGQLVLATVAP
                     EHRREAEAEAAKRKVSAQVAERSLWGVTHAEVGAFLLGLWGLPFTIVEAVANSHSPER
                     NAHERCGLPQIVWLSACLVEGDKPDPKLIERFQMSDVLAQAARIRSGQAA"
BASE COUNT          477 a          896 c          956 g          507 t
ORIGIN      
        1 cccgccgaac accgcttcga agcccagtat tcggaggatg tcttcctcgg ccgtgatcgc
       61 ttcgatgcgt ttgccgacga gctcctcttg ctctctctgg acgagctcgg agaaggcacg
      121 gttcgcggtc tcgatgcgcc cctccgcgtc gagcaccaac actccgtccg ggagctcgtc
      181 gaacgcagct ggtggcgtcc gcatgatggg gagggttcga tcagccggca tggcgaggca
      241 cctgcacgcg aatgggctcg attaggctgc ccgctcgcga gaacttgagg aggcgctcga
      301 gcagaaccat gttcagttgg cgtcccttcg gcacgacgac gacgccggtg gtcgtgcgca
      361 cgtcgtcctc gagggtcatc tctccggtca gctgcgtgac ccggagtacg cggacgttca
      421 ccgtcttggg tgacatgccg gtgaagtcgc gcagcagcgc ggccaagctg cgctcacgct
      481 cgttgtgctt cggtgcaagc ttcttgacgg actcttcgac ggtgaggccc gtttcgacga
      541 gcgcttcgac gtcgcgtgcg atccgcagca tcgcggcccc gatttcgacg tccggggtcg
      601 ggtcgggttc gttcacccgc atttggcggc gcaccatctc ggcgacctct tgaaagcgtg
      661 ggatgggcgc gagcaggcga taggcgatga gcgggtgttc ggcgaaggcc tgcttctctt
      721 cgggttccag cggaaggccg gcgatggcgc gcgtcagcgt ctcttcggga acgcccacgc
      781 aacctatcag gctgagcgcc gcggcgagct cgaacttcca gcgttgctcg tgccccagcc
      841 gctcgaccat gtgcatcacg agcatgcgaa cccgttgcgt ccgcgagaac acctgcggcg
      901 cgatcatgga gaggatctcg gtcaacagac ggatgctgcc cgtgagcgtc tctccgagca
      961 gctctcgttc gctgacgagt agccgatgct gtccggcggc ttcggtcaac gcggggagga
     1021 gcacctcggg cgcgcacggc ttgcacaaga atcggaagat gttgccctgg ttcaccgcgg
     1081 cgaccgccgc atcgagctcg gcgtggccgg tcaaaaggac gcgcgtcgtg tcgggggcga
     1141 tgcggcgcgc atgcgacagc agcgtggccc catccatgcc cggcatgcgc atatcggata
     1201 ccagcaccgc gaatggctcc tcggtttcct tcagaagctc cagcgcctgt tccggcttcg
     1261 agagcgtcac cacctcgaag tgatcgaaga gcgtacgttc gaggccttcg agcacgcgct
     1321 gttcgtcgtc gacgcacaag atccgaggaa gattggcttc ggtcatgctg cttgccccga
     1381 gcggatgcgt gctgcctgcg cgagcacgtc gctcatttga aaacgctcga tgagcttcgg
     1441 gtcgggtttg tcgccttcta cgaggcaggc cgagagccac acgatctggg ggagcccgca
     1501 tcgctcgtgg gcgttgcgct ccggcgagtg gctgttcgcg acggcctcca cgatggtgaa
     1561 cgggagaccc cacaagccga gcaggaacgc gccgacctcg gcgtgcgtca caccccagag
     1621 cgagcgttcg gcgacctgcg ccgagacctt tcgcttggcc gcctccgcct cggcctcgcg
     1681 gcggtgctct ggggcgacgg tagcgagcac gagctggccc aaatcgcaca cgagaccggc
     1741 catgaaagcg gcgtcggcgt ccaccgattt cggactgagc gcgcgcgcca ggcgcccaat
     1801 cgagagcgac ctgttctgga gctcgtcgat gaagccactc agggccttgg cggagccgcg
     1861 cgcagcttcg aatgcgccaa cgccgagggc gagatttcgg atcgtgcgag tgccgagccg
     1921 caccaccgcc gtcttgacgt cggcgatggc gtgcgacgaa gcaaagagcg ccgagttcgc
     1981 gacctgcaga atcttggtcg ccatcgctgg gtcctgctga accagatcgg cgactcgcgt
     2041 cgcatcggcg ctttcatcct cgagcagtcg cgagatctcg ctgaagagtc gaggcggaga
     2101 gggaaggtga ccgacctggc cgacgagtga ctgaagcttg gtatcgtcga ggagctgccg
     2161 aagctcacgc gtattctcga tcactcgccg cagcgttgct ccgtcgcacg gcttggcgag
     2221 gaattggtgc gctaccggaa cgacgcgcag cgcggaagcc tcgtcggtct gtccagagag
     2281 gacgattcgg atggtgcggg ggtcgagctc acagactcgg ttcagcaacg tcgcgctgtc
     2341 ttgcccgggc atgcgcatgt cgcacacgat cacgtcgacg ggtgcgctgc gaaacgcctc
     2401 gagcgctcca tcgatcgtcg atgcgaagca agtctcccag tcgacgtcga ggtcgaagaa
     2461 gaggcgttcg atggcttcga gaacgcgcgg ctcgtcgtcg acgaacagta ccctcatcaa
     2521 cgactccgat tccgggggca gggcgagcgt tcgatgaagc tcggaccttt cccagaccag
     2581 cgcttggcgg agcggcccat ttccagagat ggacggctga gacggcgccg gatttaagcc
     2641 aatcgtccgc gaatcccagg aaaatcgcgg gcgtggcgtg cttgttcggc cctcgaggtc
     2701 gctgcgcggc ctacaccaat cgcggcgcga gattgacgga attcgcgagg ttcgggcggg
     2761 gttgctggtg tccgcggccc ggcgagctaa agctcgtaca catgggcatt ttcgaccgca
     2821 tgggccgcgt catctc
//
LOCUS       DASTKS010000295         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_269600, whole genome shotgun sequence.
ACCESSION   DASTKS010000295 DASTKS010000000
VERSION     DASTKS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269600"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ35_06130"
     CDS             <1..353
                     /locus_tag="VFQ35_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9930241.1"
                     /translation="DVFFQVGLLTTMGLSAKNAILIVEFAEHAEREGKSPDDAALEAA
                     RLRLRPILMTSFAFIFGVFPLAVSTGAGAMSRVAIGTTVLGGMIAATVLAIYYVPLFF
                     VGLRRLFHRQKVQA"
     gene            350..1792
                     /locus_tag="VFQ35_06135"
     CDS             350..1792
                     /locus_tag="VFQ35_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET9930242.1"
                     /translation="MSRCRLAVATLAAVAAATLGCTLEPRYRRPKAPIAREFPSGGAY
                     GERNPTELAAVRYVDVFRDPNLRAVIASALANSRDLRVAAANVLSARAQYRAARAGLF
                     PRVDANAGVLIGGGAPSSAAGTGAATNAASPSADDIYHVYSANLALTSFELDLFGRVR
                     SSSNAALEAYFATQAGARAARLSLVSEVALQYLTLATDRSQLAIAKETLQNAGTSAEL
                     ARARLRGGIAALLDLRQAETVVLQARASIAQRTTAVEQDLNALDLLAGAPVDRARLPR
                     DLASVAALVVELPAGVRSEVLLRRPDVMQAEFLLRSANARIGAARAAFFPTISLTGLL
                     GFASTSLTNLFTSDAFGWNVRPTASVNLFNGGANQANLEYSWAQRALYLAEYERAIQR
                     AFREVADALARQGTIAEQLAAEREVVSVAEDSYRLAEARYRGGVESFLATLEAQRTLF
                     AARQSLVQTELVRAQTLVSLYRALGADSFE"
     gene            complement(1915..>2154)
                     /locus_tag="VFQ35_06140"
     CDS             complement(1915..>2154)
                     /locus_tag="VFQ35_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930243.1"
                     /translation="LGFSYTSTNVSEDLSAPGSDTTVSTSDSSSGLALNLFVPVMFHP
                     VPHFFLGFGPALDQSLTGDPKSTIVAGRLTIGGWL"
BASE COUNT          341 a          745 c          686 g          382 t
ORIGIN      
        1 acgacgtctt cttccaagtc ggcctgttga ccacaatggg tctctctgcg aagaacgcga
       61 tcctcatcgt cgagtttgcg gagcacgccg agcgcgaagg caagagcccc gatgatgcgg
      121 cgctcgaggc cgcgcggctc aggctgcggc ccatcttgat gacgtcattc gcattcatct
      181 tcggcgtctt cccgctcgcg gtctcgacgg gcgccggggc gatgagccga gtcgcgatcg
      241 gcaccaccgt gctcggtggg atgatagcgg cgacggtgct cgccatctat tacgttccgc
      301 tcttcttcgt cgggttgcgc cgcctattcc accgccaaaa ggtgcaggcg tgagccgctg
      361 ccgcctcgcc gtggcgacgc tcgcggccgt cgctgccgct acgctcgggt gcactctcga
      421 gccgcgttat cgcaggccga aagctccgat tgcgcgcgag tttccgagcg gcggcgccta
      481 cggcgagcgg aacccgaccg agctcgcggc ggtgcgttac gtggacgtct ttcgcgaccc
      541 gaacctccgc gcggtgatcg cgtccgcgct cgcgaacagc cgcgatctgc gtgtggccgc
      601 cgccaacgtg ctttctgctc gcgcgcagta ccgggcagcg cgggccggac tgtttcctcg
      661 cgtcgacgcc aatgccggtg ttttgatcgg cggtggcgct ccctcatcgg cggcgggtac
      721 gggtgccgct accaacgccg cgagcccctc tgccgacgac atctaccatg tctactcggc
      781 aaacctcgcg ctcacgagct tcgagctcga cttgttcggc cgcgtgcgaa gctcgagcaa
      841 cgcggcacta gaggcctact ttgcgaccca agccggcgcg cgcgccgcgc ggttgagctt
      901 ggtgagcgaa gtcgcactgc aatacctcac gctcgccacg gatcgaagcc agctcgcgat
      961 tgcgaaagag acactgcaga acgcggggac ctcggcggag ctcgcccgcg cgcggctccg
     1021 cggagggatc gcggctctgc tcgatttgcg gcaggccgaa accgtagttc tgcaagcccg
     1081 ggccagcatc gcccagcgca cgaccgcggt ggaacaagat ctgaacgcgc tcgacctctt
     1141 ggcaggcgcc ccagtggacc gggcgcgctt acctcgtgac ctcgcgagtg tcgcggcgct
     1201 cgtcgtcgag ctccccgcgg gggtacgctc cgaggtgctt ctccggcgtc cggacgtgat
     1261 gcaggccgag ttcctactgc gctccgcgaa cgcccgaatc ggcgccgcgc gcgccgcctt
     1321 cttcccgacc atcagcctca cgggcctgct cgggttcgcg agcacctctc tcacgaacct
     1381 gttcacctcc gacgcttttg gctggaacgt gcgcccgacg gcaagcgtca acttgttcaa
     1441 cggaggagca aaccaggcca acctcgaata cagctgggcc cagcgtgctc tctacctcgc
     1501 ggagtacgag cgagccattc agcgcgcttt tcgcgaggtc gcggacgcgc tcgctcgaca
     1561 aggcaccatc gccgagcaac tcgcggccga gcgtgaggtc gtctccgtcg ccgaagatag
     1621 ctacaggctc gccgaggctc gttatcgcgg aggagtcgag agctttctcg ctactctcga
     1681 ggcacagcgt actctctttg ccgcgcgtca atcgctcgtg caaaccgagc tcgtgcgtgc
     1741 acagacgctc gtgtcgctgt accgggcgct aggcgccgac tcgttcgagt agcctcttgg
     1801 aagcacccga ttccgcgctc gtgggtccgg actcgacccg tcgcgggcga catcgaagcg
     1861 gtgatgcccc cgcaggttcg cgagcctaga cgagtacgtc cgtaccggtc ctggctatag
     1921 ccagccgccg atcgtcaggc gccctgcgac gatggtgctc ttcggatctc ccgtcaggct
     1981 ctggtcgagc gccggcccaa aaccgaggaa gaagtgcggc actgggtgaa acatgaccgg
     2041 cacgaacaag ttcagagcga gcccactgct cgaatcgctc gtgctcaccg tggtatcact
     2101 gccaggcgcg ctcaagtctt cgctgacgtt cgtgctcgtg taggaaaacc cgag
//
LOCUS       DASTKS010000296         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_48455,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000296 DASTKS010000000
VERSION     DASTKS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48455"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFQ35_06145"
     CDS             complement(<1..665)
                     /locus_tag="VFQ35_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9930244.1"
                     /translation="MKLDDYVMLGRSGLRVSPLTLGTMTFGTDWGWGADEAGARELFD
                     RYLDLGGNFIDTADGYTGGRSETWVGKMIRERNARDRVVLGTKFTFSAEPGNPNAGGN
                     GRKNIYRALEGSLKRLGTDYVDLYWLHCWDMHTRVEEVASTLTDLVRAGKVRNIGLSD
                     VPAWYAARYQTLAEKNGWEPIAALQLEYSLIERSIEREHVPLARELGMAIMPWSPLAS
                     GFLA"
     gene            766..1725
                     /locus_tag="VFQ35_06150"
     CDS             766..1725
                     /locus_tag="VFQ35_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9930245.1"
                     /translation="MHETRGVEAFMAVAEHKSFSKAARASGVTASALSQAVRALEERV
                     GVPLFARSTRSVSLTDVGRRLFERVGPAVKETSLALEEARGAVDVAQGKLRITAGRLT
                     VPLVIEPLLPKLLREHPKLEVEIGIDDRFVDIVEMGFDAGIRLSEAIQPDFTVVRLTP
                     PFRLLVAGAPSYFEHHARPRRPRDLLEHDCINYRMASTGAYYAWEFERNRREEQVAVK
                     GRFSCNDGLTMVNAAVAGLGLVYIHEQALAAHLERGELEVVLSDYAVTVPGFFLYFPR
                     RAGIQPKLRAFIEVARSVLGPAGPVASRAALLPAKRRNARRRR"
     gene            1867..3135
                     /locus_tag="VFQ35_06155"
     CDS             1867..3135
                     /locus_tag="VFQ35_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930246.1"
                     /translation="MRKRWVVMGWALAILAGGCRDKTTSAPAPSSSTLASAPVVQAGA
                     SSAPTPHADDCSAPLDYLVEPAKYGDADLFRAIVADSTHVYFRNMRQVFRVPLAGGKA
                     EVISDAPGLTLSGKTVLFESGDRLLTQSSNEPIFMASPKSGGPWQDIINLSPKKVGGG
                     RDAATRLLQGLGSAPVPRSSQAAFDGSAFYWADNTMSRGLNVSSSATLRSVPLSGGEA
                     KELFKAQGEIGEVHRAGSRVVFVHTAPLTPQQIKSETERRKSKKLTGGERGEQRLMSV
                     PVAGGEAKELAKISSFIANLVLGTDGTDVYVSGYPNGDFNKPGVYRLNAEGGALEAVD
                     QRSVHGQAFSVGNRVIIVGSSQLEPGPGNVRRGQVVLALDRGAKSATRIACIADGYTA
                     HAFAVAGDHLLISLFKGDTNLASIARIALH"
BASE COUNT          603 a         1075 c         1104 g          579 t
ORIGIN      
        1 gcgaggaagc cgctcgccaa ggggctccac ggcatgatgg ccatgccgag ctcacgggcg
       61 agcggtacgt gctcgcgctc gatgcttcgc tcgatcagcg aatactcgag ttgaagagcg
      121 gcgatgggct cccacccgtt cttctcggcc agggtctgat agcgcgcggc gtaccaggct
      181 ggaacgtcgg agagcccgat gttccgcacc ttgcccgctc gcaccagatc cgtcagcgtc
      241 gaggccactt cttcgactcg cgtgtgcatg tcccagcagt gcaaccaata aaggtcgacg
      301 taatccgtac cgaggcgttt gagtgaccct tcgagcgctc gatagatgtt cttgcgcccg
      361 ttgccgcctg cgttcgggtt acccggctcg gcgctgaacg tgaacttggt gcccagcacg
      421 actcggtcac gggcgttgcg ctcgcggatc atcttgccga cccaggtctc gctgcgtcct
      481 ccggtgtatc cgtcggcggt gtcgatgaag ttcccgccga ggtcgaggta gcggtcgaag
      541 agctcgcgcg ccccggcttc gtccgcaccc cagccccagt cggtgccgaa cgtcatcgtc
      601 cccaaagtca gcgggctcac gcgcagcccc gagcgcccca acatcacgta gtcatcgagt
      661 ttcatcgcta gctcctaacg aacatcgtcg acgggaagct tcgggtcgcg gctgcccggg
      721 cgctagctat cgaatagctg atgcgctatt tagaaattct aatcaatgca cgaaacgcgc
      781 ggagtcgagg ccttcatggc cgtggcggag cacaagagct tcagcaaagc ggcgcgcgca
      841 tccggggtca ccgcgtcggc gctgagccaa gccgtgcgcg ccctcgaaga gcgggtcggg
      901 gttccgctgt tcgcgcgcag cacgcgcagc gtgagcctga ccgacgtcgg ccggcgcttg
      961 ttcgagcgcg tcgggccggc ggtgaaggag acgtcgttgg cgctggaaga ggcacgcggc
     1021 gccgtggacg tcgcccaggg caagctgcgc atcaccgccg gcaggctcac cgtaccgctc
     1081 gtgatcgaac cgctgctacc caagttgctg cgcgaacacc cgaagctcga agtggagatc
     1141 ggcatcgacg accgtttcgt cgacatcgtg gaaatggggt tcgacgctgg catccgcctg
     1201 agcgaagcca tccaacccga tttcacggtg gtgcggctca cgcctccgtt tcggctgctc
     1261 gtcgcgggcg ccccgagcta cttcgagcac catgctcgac ctcgccgccc gcgcgacctt
     1321 ctcgaacacg attgcatcaa ctaccgcatg gcgtcgacgg gcgcctatta cgcctgggag
     1381 ttcgagcgca atcgccgcga ggagcaggtc gcggtcaagg gtcgcttctc gtgcaacgac
     1441 gggctcacga tggtcaacgc cgccgtcgcc ggacttggcc tcgtttacat tcacgagcaa
     1501 gcgcttgcgg cgcacctcga gcgaggcgag ctcgaggtcg tgctttcgga ctacgcggtg
     1561 acggtgccag gatttttcct gtatttccca aggcgggccg ggatacagcc gaagctacgg
     1621 gccttcatcg aggtcgcgcg gagcgtgctc gggcccgcgg gtcccgtcgc gtcgcgtgcg
     1681 gcgcttctac ctgccaagcg tcgcaacgcc cgccgtcggc ggtgagccgc tttggagtga
     1741 gccgggccgc actcccaaac ccccagttat cgccacgctt gcaagagagc agcgggaatc
     1801 ggagttcgag aagttttcca ccgctcgagg aaggagcgac tgccgccgac gtagtacagt
     1861 ctcgcgatga ggaaacgttg ggtggtaatg ggatgggccc tcgcgattct tgccgggggt
     1921 tgtcgggaca agactacgag cgcgccggcg cccagtagct cgacgctggc gagcgcgccc
     1981 gtggtgcagg ccggagcgag cagcgcgcct acgccgcatg ctgacgattg cagcgctccc
     2041 ctcgactact tggtagaacc ggccaagtac ggtgacgcgg acctatttcg agccatcgtc
     2101 gccgactcga cgcacgtcta cttccgcaac atgaggcagg tgttcagggt tcccctcgcc
     2161 gggggcaagg cggaggtgat ctccgatgcg ccgggcctaa cgctgtcggg caaaacggtg
     2221 ctcttcgaaa gcggggatcg actcctgacc cagtcgtcga acgaaccgat cttcatggct
     2281 tcacccaaga gcggcggacc atggcaggac atcatcaacc tcagcccaaa gaaggtcggg
     2341 ggcggacgcg acgcggcgac gcggctcttg caagggctcg gcagcgcgcc cgtcccgcgc
     2401 tcgtcgcaag ccgcgttcga tgggagcgcc ttctactggg ccgacaatac gatgagtcgt
     2461 ggactcaacg tctcatcgtc ggcgaccttg cgcagcgtgc ccctgagtgg cggggaagcg
     2521 aaagagttgt tcaaggccca aggcgaaata ggtgaagttc accgcgccgg cagccgcgtc
     2581 gtcttcgttc acaccgcacc gttgacgccc cagcaaatca agagcgaaac ggagcgacgc
     2641 aaatcgaaaa agcttacggg gggcgagcgc ggtgaacaac gactgatgtc ggtgccggtc
     2701 gccggcggag aggcaaagga gctcgcgaag atttcgagct tcatcgccaa cctcgtgctc
     2761 ggaaccgacg gaactgacgt ctacgtctcg ggctatccga acggcgactt caacaagcct
     2821 ggtgtctacc gattgaacgc ggagggtggc gcgctcgagg cggtcgacca gcgttcagtg
     2881 cacggtcagg cgttttcggt cggcaaccgg gtcatcatcg tggggtcgtc tcaactcgaa
     2941 ccggggcccg gcaacgtgcg acgcgggcag gtcgtcttgg cgctggatcg aggcgcgaaa
     3001 tccgcgaccc gaatcgcttg cattgcggac ggctacaccg cgcacgcctt tgccgtcgcc
     3061 ggggatcatc tgctgatctc gctcttcaag ggcgacacga acctcgcgag catcgcccgg
     3121 atcgcgcttc actgagctac tgagttggcc gaggtcggta caggaccaaa agcggaacga
     3181 ggacacccct ccctgagcgc gcgaagcgcg cgattttgag aggggttggg cgcgcgcagg
     3241 ccacaggccg cgcacggacg cgggctcagg attcgtcaca tgtcgccagc aggtaggcct
     3301 tcgtctcgtt cgctcgcaga gggctcgaca cgaaagagtt ggttccgacc cgtgcttcga
     3361 a
//
LOCUS       DASTKS010000297         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_939514, whole genome shotgun sequence.
ACCESSION   DASTKS010000297 DASTKS010000000
VERSION     DASTKS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939514"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFQ35_06160"
     CDS             <1..245
                     /locus_tag="VFQ35_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET9930247.1"
                     /translation="GKSVGALESLITFVKDRPGHDQRYAVECGKIERELGWTPRHDLT
                     SGLRETVRWYLQNERWVQRVRSGAYREWLEANYGKR"
     gene            complement(270..1271)
                     /locus_tag="VFQ35_06165"
     CDS             complement(270..1271)
                     /locus_tag="VFQ35_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9930248.1"
                     /translation="MNARDPVAKNSDEPTDPTDELVATSASTAAKTDAAPPGKQLKRW
                     LQPLMWLITAAFVVWTALDLIKSWNGRRISVDPLWLSASLVPVLVGAVLQGVAWIALA
                     ERMGRTTIPRRRALSLYFASQLARYTPGKVGLPIVRMNGAPELGLSRRLVGVSVLVEM
                     LSWTATGSVVGFLMLSLSDVPSSGVGALAGKFATPLLIASLAGMVVLVAVDRRYAPAP
                     IKRALDLEGHGALVPLRLPAWQLLYWLTWALHGYFLARALGATSAEAQATMGFSPLAN
                     VLGFVALAAPAGVGVREAVLVAGLGPVIGSAGAIGAAIASRLVSLVADLGTWLALRK"
     gene            complement(1284..>3385)
                     /locus_tag="VFQ35_06170"
     CDS             complement(1284..>3385)
                     /locus_tag="VFQ35_06170"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET9930249.1"
                     /translation="SGPKLSEVARNIAKSPEDWGAIARSLTIGALLGFAAAGWVQLGI
                     SGRTASEMLASNELPEGARMMAIKVCLAAALLGALAVGGFIWNGYKKGTGVAPIERWM
                     WFLSPLLLSPAIASVLRFRPWVNQHERLLPITLVIAISLEVLACRSLVAAPRGAIEWW
                     RDARDQIPELVRRRGPFVVVLAGCIYYAVFFSLLLLRWHHKLKTGNFDLAINNNLMFG
                     GLHGHFLESPVVFPQDPKKYLANHAKFGGYLFLPIYALYPKPQTLLVMQSTFIGMSAL
                     PLWAFARRHVSDWMAAIVALAYLAYYPMHGASFSEFQYVPIASFFILTTIWAAEERRW
                     WIFGVAFLVGLTMREDIPIGMAVVGAALLLSGHRPGAGLVMAVVASIYFVVLRFYIMD
                     SAGDWWFPNMYKELWADGERGFKSVIKTLLTNPLFVLSKIFTEKKIWYLLHLFVPLSF
                     LPARRWYLWFAFIPGVLLTLLVTNYDPPTTFSFHYVMHWAPYIFVAAVLALKSLGHRS
                     DNGAQRKVAAAVGMAFASLVLSYNYGAFPRRDGSFRGGFHRVDFGISEAEEERYQNLQ
                     KLIAEIPKDASVAATEKLGPHVSSRVYMYTMRHGPQNAEYAIASNRELKLSRTRPKLL
                     EALRSGQYGVVTRIADMALMKRGYKTDDNEQLIRDWHLVDARPATPAEPAPPPRPDVE
                     GAPPPPEEGAPEPRGPE"
BASE COUNT          633 a         1081 c         1158 g          513 t
ORIGIN      
        1 cgggcaagag cgtcggcgcg ctcgagtcgc tgatcacctt cgtgaaagat cgtcccggcc
       61 acgaccagcg ctacgcggtc gagtgcggca aaatcgagcg cgagctaggc tggacgccgc
      121 gccacgactt gacctcgggg ctgcgcgaga cggtgcgctg gtacctccag aacgaacgct
      181 gggtgcagcg cgtgcgcagc ggcgcttacc gcgagtggct cgaagcgaac tacggcaagc
      241 gctgatcctc gattcctcgg ctgcaccgct cacttgcgga gcgcgagcca agtgccgagg
      301 tcggcgacga gcgacacgag ccgcgacgcg attgcggccc cgatggcgcc ggcgctccca
      361 atgacgggac cgaggccagc gacgagtacg gcttcacgca cgccgacgcc ggcgggggcg
      421 gccagggcga cgaagccaag gacgttggcg agcggcgaaa agcccatggt cgcttgcgct
      481 tcggcggagg ttgcgccgag cgcgcgcgcc aggaaatagc cgtggagcgc ccacgtgagc
      541 caataaagga gctgccaagc cggcagtcgc agcggcacca gcgcgccatg gccttcgaga
      601 tcgagcgcgc gcttgatggg cgcaggcgcg tatcgccgat cgacggctac gagtacgacc
      661 atccctgcga gcgaagcgat gagcaacggc gtcgcgaatt tgccggcgag cgctcccacc
      721 cccgaactcg gtacgtccga cagcgagagc atgagaaagc ccacgacgct gccggtcgcg
      781 gtccacgaga gcatttcgac cagcaccgac accccgacca agcggcgtga gagaccgagc
      841 tcgggcgcgc cgttcatgcg cacgatgggc aagcccactt tgccgggcgt gtagcgcgca
      901 agctgggaag cgaaatagag cgagagcgcg cgccgccgcg ggatggtcgt tcgccccatg
      961 cgctcggcga gcgcaatcca ggcgacacct tggagcacgg cgccgacgag cactggcacg
     1021 agcgaggcac tcagccagag cggatcgacg ctgatgcgac gcccgttcca gctcttgatg
     1081 aggtcgaggg ccgtccagac gacgaacgcg gccgtgatga gccacatcaa cggctggagc
     1141 caacgcttca gctgcttgcc cggcggagcc gcatcggtct tggcggcggt gctggcgctc
     1201 gtggcgacga gctcatcggt tggatcggtg ggctcgtcgc tgtttttggc gaccgggtcg
     1261 cgcgcgttca cgagcgccgc cctctattcc ggtcctcgcg gctcgggggc gccctcttcg
     1321 ggcggcggcg gagcaccttc cacatcgggt cgtgggggag gggctggttc ggcgggcgtc
     1381 gcgggccgcg cgtccacgag gtgccagtcg cggatgagct gctcgttgtc gtccgtcttg
     1441 tagccgcgct tcatcagcgc catgtcggcg atgcgcgtga cgacaccgta ctggccgctg
     1501 cggagtgcct cgagcagctt cgggcgcgtt cgggagagct tgagctcgcg gttcgaggcg
     1561 atcgcgtact cggcgttctg cggaccgtgc cgcatcgtgt acatgtagac gcgcgaggag
     1621 acgtgcggcc cgagcttctc ggtggcagcc accgacgcgt ctttcgggat ctcggcgatg
     1681 agcttctgca ggttttggta gcgctcctcc tcggcttcgc tgatgccgaa gtcgacgcgg
     1741 tggaagccgc cgcggaagct gccgtcacgg cgtggaaacg cgccgtagtt gtagctgagc
     1801 acgaggctcg cgaacgccat gccgacggcg gccgcgacct ttcgctgcgc gccgttgtcg
     1861 ctgcggtgtc ccagcgactt gagcgccagc accgcggcca cgaagatgta aggcgcccag
     1921 tgcatcacgt agtggaacga gaaggtggtc ggtggatcgt agttcgtgac cagcagcgtg
     1981 agcagcacgc cggggatgaa cgcgaaccac agataccaac gccgcgccgg caagaagctg
     2041 agcggtacga acaggtggag gaggtaccag atcttcttct cggtgaagat cttgctgagg
     2101 acgaagagcg gattggtgag cagcgtcttg atgacgctct tgaagccgcg ctcgccgtcg
     2161 gcccagagct ccttgtacat gttcggaaac caccagtccc cggcggagtc catgatgtag
     2221 aagcgaagga ccacgaagta gatgctcgcg accacggcca tgacgaggcc agcgccgggg
     2281 cggtgtccgc tcagcaagag cgcggctccg accaccgcca tcccgatggg gatgtcctcg
     2341 cgcatggtga ggcccaccaa gaaggcgacg ccgaagatcc accaacgccg ctcttctgcg
     2401 gcccagatgg tcgtcaaaat gaagaagctc gcgatcggaa cgtactgaaa ctccgagaag
     2461 ctcgccccgt gcatcgggta gtacgcgagg tacgcgagcg cgacgatcgc cgccatccag
     2521 tcggagacgt gacggcgcgc aaaagcccag agcggcagcg cgctcatgcc gatgaacgtc
     2581 gactgcatca cgagcagcgt ttgcggcttc gggtacaacg cgtaaatggg caaaaagagg
     2641 tatccgccga acttcgcgtg gttcgccagg tacttcttcg ggtcctgcgg aaacaccacc
     2701 gggctctcga ggaagtgccc gtgcagaccg ccgaacatca ggttgttgtt gatcgcgaga
     2761 tcgaagttgc cggtcttgag cttgtggtgc cagcgcagca agagcagcga gaagaacacc
     2821 gcgtaataga tgcacccggc caacacgacc acgaatgggc cgcgccgcct gactaactcc
     2881 gggatttgat cacgcgcgtc gcgccaccac tcgattgcgc cacgcggggc cgccacgagc
     2941 gaccgacagg cgagcacctc gagcgagatc gcgatgacga gcgtgatcgg caacaagcgc
     3001 tcgtgttggt tcacccaggg cctaaacctg aggaccgaag cgatcgccgg cgagagcaag
     3061 agtggcgaca gaaaccacat ccagcgctcg atgggcgcga cgccggttcc cttcttgtag
     3121 ccattccaga tgaagccgcc gaccgccagt gcgccgagaa gcgctgcggc gaggcacacc
     3181 ttgatggcca tcatgcgagc gccttccggc agctcgttcg aggcgagcat ctcgctcgcg
     3241 gtacgaccac ttatgccgag ctgaacccac cccgccgccg cgaagccaag cagcgcgccg
     3301 atcgtgagac ttcgcgcgat ggctccccag tcttccggcg attttgcgat gtttcgcgcg
     3361 acctcggaca gcttgggacc cgact
//
LOCUS       DASTKS010000298         7576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1610193, whole genome shotgun sequence.
ACCESSION   DASTKS010000298 DASTKS010000000
VERSION     DASTKS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610193"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFQ35_06175"
     CDS             <1..885
                     /locus_tag="VFQ35_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9930250.1"
                     /translation="PIVGYLVSRPHAPYFMSWAGFLFDSSIPWLLLHRRTRLLAYAFV
                     LLFHLVTRTLFPIGMFPVIMVLSALVFFPASFPRALLRVFRPNLRSASPVKSQSSLLQ
                     NEGAPPVTSARLPLNGKVRVALALGVAYCAFQAVFPLRFLAYGGNVRWHEQGMRFSWR
                     VMVREKNGALTFVVRQKSTGRVYHVSPRRYLTRLQEREMSAQPDLILQLAHHVRDDFR
                     RRGLGDVEVRADSWVSLNGRRMARLVNPDVDLTSVEDGLARAGWILPAPDSPPPRVRV
                     NATSAVSAAATHEPGANG"
     gene            complement(1074..3341)
                     /locus_tag="VFQ35_06180"
     CDS             complement(1074..3341)
                     /locus_tag="VFQ35_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9930251.1"
                     /translation="MEAAGGAAGGNESSWVSATAAPITATSVPPEASSASAPPAAITA
                     TSVPPEASSASATRSEAPVTEVTVLGSRTRRTAGADYVISTRHRERQKYDDPLAVLRM
                     APGVVARTEDGMGLRPNIGLRGANPDRSKKVALMEDGILIAPASYSAPAAYYFPMIQR
                     MAQIRVVKGPGAIAYGPQTVGGAIDLVTRPIPSSLSGAVDLGFGAYGYAKAHAHAGFS
                     DGRTGFLLEGVHLRSSGWKELPSGADTGFYRNEWMVKASHVLDPSARERHEFRLKATY
                     SEEASNETYLGLSDADLRRNPNRRYPASALDHMRWHRTMVVAEHEYRPSERLTITSSV
                     YRSDFSRRWRKLNHFRGANLFDVLQDPTSPQNAIFYGVLKGEQDSSTPAEALFIGPNQ
                     RDFVSQGVQTRAKLTLRTGPVSHKIEYGLRLHYDRIERHHSEDAFSMVGGELVPEGSP
                     TIVTVFNEAWTESVALHAVDTMTWKGLSITPGVRVELMRSALIDKAAGTREQGASQAL
                     LPGIGVYQAITEELGVLAGVYRGFSPPPPGSTTLKPELSINYEAGVRHSQRHMRAEVI
                     GYLNDYQNLTDVCTLSSGCLDADLDRQYPAGRARIYGLEAFIDRDIPVGPVKVPVLAS
                     YTLTRATFSRSFSSDDPIFGTVHEGDEMPYVPRHQLSATLGIQAERGGVYASMLYVSR
                     MRERAGSGAYDPKLTTDAEFGVDVSGQLRPLSFLELYLNVRNVFDNQPLLARRPFGAR
                     PSAPRWVQIGAKISF"
     gene            4276..4515
                     /locus_tag="VFQ35_06185"
     CDS             4276..4515
                     /locus_tag="VFQ35_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930252.1"
                     /translation="MSSRNEHRTPERIAQGHIVAVDQCDCGTLHVHIGAVSLRLDVPA
                     LRDLLSTLGQALAVRTAADYTTQALSLSGTKRGIG"
     gene            5200..5616
                     /locus_tag="VFQ35_06190"
     CDS             5200..5616
                     /locus_tag="VFQ35_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930253.1"
                     /translation="MTNCCGAMLVMAIATSEKAAFDSAEAICDEQYPACGCAAQGVDV
                     EDGTRVGWPWQDAVKASCDAGSCKAHYAGATFSCGARTCTDKEYCNVTSGGPAGSEPT
                     STCTQTTCVDCACLKVDVACSCSANNGHLSVSCQRP"
     gene            complement(5632..>7576)
                     /locus_tag="VFQ35_06195"
     CDS             complement(5632..>7576)
                     /locus_tag="VFQ35_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930254.1"
                     /translation="MQWSDVATIDLLSRLGQREERAKLLVIATARHAELQSPDHPLNP
                     VVRSLVARSGALSLQLPKFGVVSVQSFIDRRFPGHDFPSELTDLVAKISGGTPLFMAS
                     LLDELAGRKMFAERDGGWRLTVSLAEVQAHRPASIKQLIDMQLDRLPLAAQRVLEAAS
                     IVGAEFSTELVAAALELSVEQVDDVCDSLARQAQFIHAEPEARYAVNHALVQEVCVER
                     SSPARRQRWHRLVAEAIERDPRASELSHLLAKHFDAAGDAGRAVAAYTTAAHQAARRY
                     ATSDTIALCARALDLLPRMAPSHERDLLEFQILETMCDQVSSNSFKATFAGREPLAVY
                     GRAIEIARSLGDSARVYAAVTQLCTYYMIIAQYTRAAEQARELEQIEQTHELSAELLH
                     SGIFARAYTAFFSGDLATAIHLLERLAPQNQAASVFRDNLAGRALALGHLACARWVAG
                     EPERALEEALETISLAERVKVPVLVALAHVVRARLRYLRRDPLPVVEEEALLAVRAAA
                     ADLGLLTEANAFALLAEAQRAPLALAAIDPLLLALRQRLAEVATCSTLVALVLIDVLR
                     ISGHHEQARALTDEIIAFALAHQENVYLPELLRVRGEQRQSADPDAAARDYRDAIELA
                     QRLGARSFETRAAESLAAVGSSS"
BASE COUNT         1306 a         2438 c         2490 g         1342 t
ORIGIN      
        1 cccatcgtcg gctacctggt gtcgcgcccg catgcgccct atttcatgag ctgggccggt
       61 tttctgttcg atagctcgat cccgtggctt ctgcttcatc gtcgcacgcg gctccttgcc
      121 tacgccttcg tactcctctt ccacctggtc acgcgcacgc tcttcccgat cggcatgttc
      181 ccggtgatca tggtgttatc ggcgctcgtc ttcttccctg cgagctttcc gcgcgccttg
      241 cttcgcgtgt tccgccccaa tctgcggtca gcctcccccg tgaagtcgca gtcctccctg
      301 ctacagaatg agggggcacc cccggtcacg agcgcgcgac tcccgttgaa cggtaaggtt
      361 cgcgtcgccc tcgcgttggg cgttgcgtac tgcgcgtttc aagccgtatt tccgctccgc
      421 tttttggcct atggggggaa cgtgcgctgg cacgagcagg gcatgcgctt ctcgtggcgc
      481 gtgatggtcc gagagaagaa cggcgccttg accttcgtcg tgcgtcaaaa gtcgaccggg
      541 cgcgtttatc acgtcagccc acgacgctac ttaacgcgcc tccaagagcg cgagatgtcg
      601 gcgcagcccg acctcatcct gcagctcgcg caccacgtcc gggacgactt ccggcgacgc
      661 ggccttggcg acgtggaagt gcgcgcggat tcgtgggttt cgctcaacgg tcggcgcatg
      721 gcgcgtctcg tcaatcccga cgtggatctc acgagcgtcg aggacggatt agctcgcgcc
      781 ggctggatac tacccgcacc cgactcacct ccgccgcgcg tgcgagtgaa tgctacgagc
      841 gccgtctctg ccgcggcaac gcacgaaccc ggcgccaacg gctgagcgct ctgcctcgtc
      901 gcgctctgag ctcgtctggc tctgtaccgt cgcgctttgc ctcgtcgcgc tccgcgctcc
      961 tctggcttgt gcaacgtcgc gctcggcgtc gctgctctct gccgcgactg gttcacgccg
     1021 tcggctccgc gcccctgctt cgcggaagcc gcagcgtacg gggctcctcg tctctagaag
     1081 ctgattttcg ctccgatctg cacccagcgc ggtgctgaag gacgggcgcc aaacgggcgc
     1141 cgcgcgagca gcggctggtt gtcgaagacg tttcgcacgt tgaggtaaag ctccaggaac
     1201 gagagcggcc gtagctgccc actgacgtcc acgccgaatt ccgcgtcggt cgtgagcttc
     1261 ggatcgtagg cgccgctgcc ggctcgttcg cgcatgcgcg acacgtacaa catgctcgcg
     1321 tagacgcccc ctcgttccgc ttgaataccg agcgtcgcgc tgagctgatg gcgagggaca
     1381 tagggcatct cgtcaccctc gtgcaccgtg ccaaagatgg gatcgtcgga gctgaacgag
     1441 cgggagaacg tcgcgcgcgt caatgtgtag ctcgccaaaa ccggaacctt cactggaccg
     1501 accggtatat cgcggtcgat gaaggcttcg agcccgtaga tgcgcgcccg cccggctggg
     1561 tattgtcggt ccaagtcggc gtcgaggcag ccactcgaaa gagtgcacac gtcggtcagg
     1621 ttttggtagt cgttcaagta gccgatcact tcggcgcgca tgtgacgctg cgagtgacgc
     1681 acgccggctt catagttgat gctgagctcc ggcttcagcg tcgtactgcc cggcggcggg
     1741 gggctgaacc cgcggtacac gccggccaac accccgagct cctccgtgat cgcttggtac
     1801 acgccgatgc cgggaagcaa ggcttgcgac gcaccttgct cgcgcgtgcc ggccgcctta
     1861 tcgatgagcg ccgaacgcat cagctcgacg cgaacaccag gggtaatgct gagccctttc
     1921 cacgtcatcg tgtcgactgc gtgaagcgca accgactcgg tccacgcttc gttgaacacc
     1981 gtcacgatgg tcggacttcc ctcggggacg agctccccgc caaccatcga gaaggcgtct
     2041 tcgctgtgat ggcgctcgat gcggtcgtaa tgcaaccgca ggccatactc gattttgtgc
     2101 gagaccggac ctgttcgtag tgtcaacttg gcccgggttt ggacgccttg cgaaacgaag
     2161 tctcgctggt tcggtccgat gaagagcgcc tcggcggggg tgctgctgtc ctgctcgcct
     2221 ttgagcacgc cgtagaagat cgcattttgc gggctggtag ggtcctgcaa gacgtcgaac
     2281 aggttggcgc cccgaaagtg attcagcttg cgccagcgtc gcgagaagtc ggagcggtaa
     2341 accgaagagg tgatggtcag gcgctcgctc ggccgatact cgtgttcggc gaccaccatc
     2401 gtacgatgcc agcgcatgtg atcgagcgcg ctcgcaggat aacgccggtt cgggttgcgc
     2461 cgcagatccg cgtcggagag cccgaggtac gtctcgttcg acgcctcttc ggaatacgtc
     2521 gccttcaagc gaaactcgtg ccgctctcgc gcgctcggat cgagcacatg cgacgccttg
     2581 accatccact cgttccgata aaagccggtg tccgctccgc tcggcaactc cttccacccc
     2641 gagcttcgca ggtgcacgcc ttcgagcaga aagccggtgc gaccatcact gaagccggcg
     2701 tgagcgtgcg ctttcgcgta accgtacgca ccgaatccca gatccaccgc gcccgaaagc
     2761 gagctcggta tgggtcgagt gacgagatcg atcgcgccgc ccaccgtttg cgggccatac
     2821 gcgatcgcgc cgggaccctt gacgacgcgg atctgcgcca ttcgctggat catcgggaaa
     2881 tagtaggccg ccggcgccga gtaagaggcc ggcgcgatca agatcccgtc ctccatcagg
     2941 gcgactttct tgctgcgatc cgggtttgca ccgcgcagcc cgatattggg acggagcccc
     3001 atgccatcct ccgtgcgcgc gaccacgccc ggcgccatcc gcaataccgc gagcgggtcg
     3061 tcgtactttt ggcgctcgcg atggcgggtg gaaatcacgt agtccgcacc cgccgtgcgc
     3121 cgagttcgcg aaccgagcac cgtcacctcg gtgaccggcg cttcgctgcg cgtcgccgac
     3181 gcagaagacg cttcgggagg cacgctggtc gcggtgattg ctgccggagg cgccgacgca
     3241 gaagacgctt cgggaggcac gctggtcgcg gtgattggcg ccgcggttgc cgagacccaa
     3301 ctcgactcat taccgcccgc agcaccaccc gcagcctcca tgccgcgttc ctctgccggg
     3361 accttcgtgc ccgcaccgtc ggaaggcgca gcctgggtta actctggagc ttccgcgctc
     3421 gccgtttccg acccgtgtgc tcgtgatgct ttttgcggcg gggcggctgc cactctgcgt
     3481 cgcgcggcac ctcgtggaga ggcttgcatc ggcggcgcac tcttcgccgc agcacctccc
     3541 cgcgccgtac ccgccgacga cgcttcgctc ggagcggcac ctcctggcgc ggcctcggcc
     3601 cggctcggcg cgaggagact cagcaacagc aatggcgcga tgaattcaca gtcgaatggg
     3661 cgaaacatcg ttgaagaacg acctcgcaag taacagttcg tccccgcaat tggaagaagt
     3721 aaatgaaaat ggagttgaat atcattttca tttactccga atggcgctac aagggacagg
     3781 tgatgagcca gcagatcacc gcctgcacgc gtagccgggc tcagcaagcc gagcgacgcc
     3841 gcgctccgct caagaagcca cgcctggacg cggggcgctc ggcgagcagg gagtccgcgc
     3901 gacgctggac ctcgattcac tcatcgatgc ttttcgcgtt actgctcgtt ctgagcgcat
     3961 cgcatcccgc agtagcaggg acttctctcc gcccagcacg cgagccaatt cacgcgctcg
     4021 gccgtcctcg cctcgagttc gtgctccttt ctgtcttact cggcgtgatg acaggacgag
     4081 ctctagagcc aggtctgaat atgggaccga gccccgcgaa gagacgcgag cggtctgagg
     4141 cgagacgacg agacgacgga aagctcgccg aagagaagca acggacgaac gcgaagcgaa
     4201 tcgggcgtgg ggtgacggga gagactcacg aacggctttt ggatcaccac gtctcgaacg
     4261 acaggagagc agaccatgag ttcacgcaac gaacaccgta caccggaacg catcgcgcaa
     4321 ggtcatatcg tcgccgtaga ccagtgtgat tgcggaacac tgcacgttca tatcggcgcg
     4381 gtcagtctac ggctcgacgt gcctgccctg cgcgacttac tgagcacgct aggacaagcg
     4441 ctggcagtgc gaactgcggc cgactacacg acgcaggcgc tttcgctgag cggcaccaaa
     4501 cgcgggatag gatgactcgg agctggtgcg cagccagaag cagtggcctt tgccgctgcg
     4561 agaatggccc aaggtgtgcc accccacggc aacctctcac ctgtaagtag tcaaaaacat
     4621 tcactcgcgg ggtggcacgc ggcctgctac tcgcagcgcc caatgcgttc cctttcgccg
     4681 ttcaggttcc tcacgtcggt tcgcgtagct caggtgaagg gcgatcgaga tgcgaggcga
     4741 ggagcgcgag ttagcgtaac ggccgcgctc gtttgcagct gtgcattccc aattgcgtgt
     4801 ggcggcgagc aagcgggagg tggcccctca ggctctggtg gatcagccgc aggcgggtct
     4861 acgagcgcat cgggcgggac gaccggaggc gcgaaagcaa gcggcggagc gagcgccggc
     4921 ggctcggcag cggctggagt cggcggccac acggggcagg gcggtgcagc ttcgggcggt
     4981 ggccgtgccg cgacgggcgg tgcagcttcg agcggtggcc gtgccgcaac gggcggtacc
     5041 ggctccgcat cgggcaattc aggcgctgga ggcagcggcg aagcgggggc cggcgggagc
     5101 gcgggcgaca gcgggatctc ctgctggggc acgacgcgtt ctttcccgga gttcaatcgg
     5161 gggtgcagcg ccgcttccga ctgcgcgctc gtggctcaca tgaccaattg ttgtggcgcg
     5221 atgctcgtca tggcgatcgc cacgtcggaa aaggctgctt tcgacagcgc ggaagcgatt
     5281 tgtgacgagc agtacccggc gtgcggatgc gctgcgcagg gcgtcgatgt cgaagacgga
     5341 acccgcgtcg ggtggccctg gcaggatgcg gtgaaggctt cgtgcgacgc gggaagctgc
     5401 aaggcacact acgcgggagc aaccttctcg tgcggcgcac ggacctgcac cgacaaggaa
     5461 tactgcaacg tgacgtcagg cggacctgct ggttccgagc cgacgtcgac ctgcacgcaa
     5521 acgacttgcg tggactgcgc ctgcttgaag gtcgacgtcg cgtgctcgtg cagcgcgaac
     5581 aacggccacc tctccgtgtc gtgtcagcgt ccgtgaatta gtcaggcttc gtcacgagct
     5641 cgagccaact gccgccaggc tctccgccgc gcgggtctcg aagctgcgtg cgcccaaccg
     5701 ctgcgctaac tcgatggcgt cccggtaatc tcgcgcggcc gcgtcgggat cggcgctttg
     5761 ccgctgctcg ccacgcactc gcaagagctc gggtagatac acgttttctt ggtgtgcgag
     5821 cgcgaaggcg atgatttcgt cggtgagggc acgagcttgc tcgtggtgcc ccgagatgcg
     5881 aagcacatcg atgagcacca acgcgaccaa ggtcgagcac gtcgcgactt cggccagcct
     5941 ttggcggaga gcgaggagca atgggtcgat ggcggcgagc gcgagcggag cacgctgcgc
     6001 ttccgccagt agcgcgaacg cgttcgcttc ggtgagcagg ccgagatccg cggcggcggc
     6061 gcggacggcg agcagcgcct cctcttcgac gacgggcaac ggatcacggc gcagatagcg
     6121 cagccgtgcg cgtacgacgt gcgccagcgc cacgagcacc gggaccttca ctcgctcggc
     6181 gagtgaaatc gtctcgaggg cttcctcgag cgcacgctct ggttcacctg cgacccaacg
     6241 cgcgcaagcg aggtggccga gcgccaaagc tctgccggcg aggttatcac gaaagacgga
     6301 cgctgcttgg ttctgcgggg ccagccgctc gaggaggtga atcgccgtgg ccaagtctcc
     6361 gctgaagaac gccgtgtacg cgcgggcgaa gatgcccgag tgaagaagct cggcgctgag
     6421 ctcgtgcgtc tgctcgatct gctcgagttc gcgggcttgc tcggcggccc gagtgtactg
     6481 cgcgatgatc atgtaatacg tacaaagttg agtgacggct gcgtacactc gcgcggagtc
     6541 gcccagtgac cgcgcgatct cgatcgcccg cccatagacc gcgagtggct cgcggccagc
     6601 gaaggtcgct ttgaaggagt tcgagctcac ttggtcgcac atcgtctcga gtatctggaa
     6661 ttcgagcaga tcgcgttcgt gactgggagc catacgcggc aacagatcga gcgcgcgtgc
     6721 gcagagcgcg atggtatcgg aggtcgcgta gcggcgggcg gcttggtgcg cggcggtcgt
     6781 gtaggccgcg actgcacgtc cggcatcgcc tgcagcgtcg aagtgcttgg cgagcaaatg
     6841 cgagagctcg ctcgctcgcg gatcgcgctc gatcgcttcc gcaacgagcc gatgccagcg
     6901 ctgacgacgc gccggactac tgcgctcgac gcagacctcc tgcacgagcg cgtggttcac
     6961 ggcgtaacga gcttcgggtt cggcgtgaat gaactgcgct tgacgcgcga gcgagtcgca
     7021 aacgtcgtcg acctgctcga ccgagagctc gagcgccgcg gcgacgagtt cagtcgagaa
     7081 ctcggccccg acgatactgg ccgcttcgag cacgcgctgc gccgcgagcg gcaaccgatc
     7141 gagctgcatg tcgatcagct gcttgatgct ggcggggcga tgagcctgga cttcggcgag
     7201 ggagaccgtc agcctccagc ctccatcgcg ctcggcaaac atttttcgac ccgcgagctc
     7261 gtccagcaac gaagccatga aaagcggcgt tcccccgctg attttcgcga ccaagtcggt
     7321 gagctcggag gggaaatcgt gccctggaaa gcgcctgtcg atgaagctct gcaccgacac
     7381 cacgccaaac ttcggaagtt ggagcgaaag cgcgccgctg cgagccacga gcgatcgaac
     7441 gacggggttg agcggatggt ccgggctctg gagctccgca tggcgcgcgg tggcgatgac
     7501 gagcaacttg gcgcgctcct cacgctggcc gaggcgagac aacaaatcga tcgtggccac
     7561 gtccgaccac tgcatg
//
LOCUS       DASTKS010000299         4802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_716956, whole genome shotgun sequence.
ACCESSION   DASTKS010000299 DASTKS010000000
VERSION     DASTKS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716956"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFQ35_06200"
     CDS             complement(<1..888)
                     /locus_tag="VFQ35_06200"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9930255.1"
                     /translation="MSSPFIPVCEPLLLGNELKYVTDAVSKGWISSSGGYVHEFEKKF
                     AEYLGVRHAVTVCNGTVALHLALVAAGIGEGDEVIVPTFTMFASCSAICYTGAKPVFV
                     DCERDSWNLDVTQLEAKITSRTRGIMPVHIYGLPVDMDPLLELAKRYNLLVFEDAAEA
                     IGSEYKGKKCGALSDFGCFSFFANKVITTGEGGMVVTNDDKYAEQLRYYKNLAFPLKG
                     PRRYIHEHIGFNYRMPNTSAAIGLAQLERVDDYVDRRRNNARRYNERLRGKRGITVPA
                     ERSWAKNTFWMYSILIEDDF"
     gene            complement(980..1387)
                     /locus_tag="VFQ35_06205"
     CDS             complement(980..1387)
                     /locus_tag="VFQ35_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930256.1"
                     /translation="MQGVEIFRAGEQVLAILVRANATSTEKYNFLTQQSEPLQLGMNF
                     YKAGEKIATHTHLPRDIRIERVQEMIVIGEGRTRLSLYDDARVKVAETELGKGDIVLL
                     ASGGHGFDVLEDTKIVEVKQGPYDGKQKDKVAI"
     gene            1521..2621
                     /locus_tag="VFQ35_06210"
     CDS             1521..2621
                     /locus_tag="VFQ35_06210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9930257.1"
                     /translation="MRIAVVGPTYPYKGGIAHHTTELAHRLAAAGHEVELLSWSAQYP
                     DFLYPGVQRAGDDEPETPPFPNTEYPLSWKNPAGWVLTGRRLRSFDRIVLVFVTSIQA
                     PAQLSMLAAMGPAARRRTVALCHNVLPHERRFFDVPLTRALLSSVGSVLVHTTAQAEL
                     ARSLGARDVRSADMPPHFPARAPAPTSAADEPLKRRLLFFGLVRPYKGVDVLLSALAG
                     VPDVALTIAGEFWGGAGEYERLVRELGLAGRVTLESGYVPSRRIPELLAACDALVLPY
                     RSGTATQNVKLAHAHGVPVIATRVGSLPTQVRDGVDGLLCSPDDVDDLRRKLERFYEP
                     GVAAELRRGVPPVSSDAEWASYLPVLLAGEPR"
     gene            2826..3578
                     /locus_tag="VFQ35_06215"
     CDS             2826..3578
                     /locus_tag="VFQ35_06215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9930258.1"
                     /translation="MSVFGSYSRYYDLLYKDKDYGAEAEYVHALISQHCPGSKTILDL
                     GCGTGRHAQLFANKGYSVTGLDRSEQMLETARAASQGSAPEYLEGDLRDARLGRDFDV
                     VVSLFHVMSYQTSNADLRGAFTTVREHLRPGGLFLFDCWYGPAVLTQRPQVRIQRLED
                     EHVSVTRLAEPTMRPNENLVDVNYHIFIEDKLSQRVDQLSETHTMRYLFVPEVELLFE
                     SAGLKLRHACEFMSAKEPSLDTWNVMFMGTRG"
     gene            3631..4605
                     /locus_tag="VFQ35_06220"
     CDS             3631..4605
                     /locus_tag="VFQ35_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9930259.1"
                     /translation="MNGRQALKYFVYAAVLGGIGFFFYRAFEKNWDKVAAHQFKLDYV
                     ALALALFTLVATYIVSMYGWHRSINALSRGPQLTISQSLAAVNSSNLTKYLPGKVWSY
                     AFQLYWLSGAGFSKALITYVNVINLAISIVTTLIAGFFLLLFNPGKLGFGATLAALVG
                     VVVADLVAVKFHAPLIRFGLSQLGRLLKRELTYFEPPPRVWLELHAAHLAAAFTFGLA
                     AYFTSLAIGYSLPLSQAPLVMAALLIADLVGFVAIFVPGGLGVREGFMYVMLGGAGSG
                     SLALTLPLAIRMLNMVVDLGLGGLALHLSRGFARAKPGAPPPPQEIPT"
     gene            4602..>4802
                     /locus_tag="VFQ35_06225"
     CDS             4602..>4802
                     /locus_tag="VFQ35_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930260.1"
                     /translation="MTASASRLDVALLCFPYFPSKDTGRGHDRYVYELAENLKKLEPA
                     LGVQVIEQGFSKGLSAAFGKLSK"
BASE COUNT          777 a         1560 c         1518 g          947 t
ORIGIN      
        1 gaagtcgtcc tcgatcagaa tcgagtacat ccagaaagtg ttcttcgccc acgaacgctc
       61 ggcggggacg gtgatgccgc gcttgccgcg cagccgttcg ttgtaacggc gcgcgttgtt
      121 gcggcgccgg tcgacgtagt cgtcgacgcg ctcgagctgc gcgagaccga tggcagccga
      181 agtgttcggc atgcggtagt tgaagccgat gtgctcgtgg atgtagcgcc gcggcccctt
      241 cagggggaag gccaggttct tgtagtagcg cagttgctcc gcgtacttgt cgtcgttggt
      301 gacgaccatg ccgccctcgc ccgtcgtgat gaccttgttg gcgaagaagc tgaagcagcc
      361 gaagtcgctc agggctccgc acttcttacc cttgtactcg gagccgatgg cctcggcggc
      421 gtcctcgaag acgagcagat tgtaccgctt ggcgagctcg agcagcgggt ccatgtcgac
      481 gggcaagccg tagatgtgaa ccggcatgat accgcgcgtg cggctcgtga tcttcgcctc
      541 gagctgcgtc acgtcgaggt tccagctgtc gcgctcgcag tcgacgaata cgggcttcgc
      601 acccgtgtag caaatcgccg aacacgaggc gaacatcgtg aacgtgggga cgatgacttc
      661 gtcgccttcg ccgatgccag ccgcgacgag cgcaaggtgc agcgccaccg tgccgttgca
      721 caccgtaacc gcgtgacgaa ccccgaggta ctcagcaaac ttcttctcga actcgtgaac
      781 gtacccgccc gaagacgaga tccatccctt ggaaacggcg tcggtcacgt acttgagctc
      841 gttgccgagg aggagcggtt cacaaacggg aatgaaaggt gagctcattg gggtgttctc
      901 tttttgcgct cgcgcgaggg ttcggtgtgc ggatggtcgg taaggtttgc gctttgggac
      961 ggtggcccga gacctgacgc tatatcgcga ccttgtcttt ttgcttgccg tcgtatgggc
     1021 cctgtttgac ctcgacgatc ttggtgtctt cgagcacgtc gaagccgtgc ccgcccgacg
     1081 cgagcagcac gatgtctccc tttccgagct cggtctccgc caccttgacg cgcgcgtcgt
     1141 cgtagagaga gagccgcgtg cggccctccc cgatcacgat catctcttgg acgcgctcga
     1201 tgcggatgtc gcgcggcaaa tgcgtgtgag tcgcgatctt ttccccggcc ttgtagaagt
     1261 tcatgccgag ctgcaaaggt tcgctctgct gcgtgaggaa gttgtatttc tcggtgctcg
     1321 ttgcgttggc gcgcaccaaa atcgcgagca cctgctcgcc cgcgcggaaa atctcgactc
     1381 cttgcatggg gcatcctcta ccacgaccga gaagcgccga acgagctcgt cgcaccgatc
     1441 cgaatcggcc aggcgaccgg cgcagttggc gctaattccg cgtcggatcc gcccatcgga
     1501 gtgctaggag ggtgccggca atgcgcatcg ccgtcgtggg accaacctac ccttacaagg
     1561 gcggcattgc tcaccacacg accgagctcg cgcaccgctt ggccgccgcc ggacacgagg
     1621 tcgagctcct gtcttggtct gcgcaatacc cggatttctt gtacccaggc gtgcagcgcg
     1681 cgggcgacga cgagcccgag actcccccct ttccgaacac cgagtacccc ctctcttgga
     1741 agaacccggc gggctgggtt ctgacgggac gacggcttcg aagcttcgat cgcatcgtcc
     1801 tcgtcttcgt cacgtcgatc caagcgcccg cgcagctttc gatgttggcc gcgatggggc
     1861 cggcggcgag gcgacgcacc gtagcgctct gccacaacgt actcccccac gagcgacgct
     1921 tcttcgacgt cccgttgacg cgcgcgctct tgagcagcgt cggcagcgtg ctcgttcata
     1981 cgaccgccca agccgagctt gcgcggagcc tcggcgcccg cgacgtgagg agcgcggaca
     2041 tgccgccgca ctttccagcg cgagccccgg cgccgacgag cgccgccgac gagccgctca
     2101 agcgcaggct tttattcttc ggtctggtgc gtccctacaa gggcgtcgac gtgctgctct
     2161 ccgcgctcgc cggggtgccc gacgtcgcgc tcacgatcgc aggtgaattc tggggcggcg
     2221 ctggcgagta cgagcggctc gtgcgtgaac tcgggctcgc gggtcgagtc actctagaaa
     2281 gtggttacgt cccttcacgt cgaattcccg agctcttggc cgcgtgcgac gcgctcgtgc
     2341 tgccgtaccg gagcggaacg gcgacgcaga acgtaaagct cgcgcacgcg cacggagttc
     2401 ccgtcatcgc gacgcgcgtc ggctccttgc cgactcaagt gcgggacggc gtagacggac
     2461 tgctctgcag ccccgacgac gtcgatgacc ttcgccgaaa gctcgagcgc ttctacgagc
     2521 ctggggtcgc agccgagctt cgccgcggcg ttcctcccgt ttcgagcgac gccgagtggg
     2581 ctagctatct gccggttctg ctagccggag agcctcgatg atcgacgggg cccctcgccg
     2641 ctcgcgtgat gccgcgactt ttcgtgggcg gccacgcggc tcgttgctcc gaaacccctc
     2701 ttctctggta ttatttgggc cgatgcaggt aacgctgacg ttggcacatc ccccaatggg
     2761 cgggcgagct catccttcga attgccggga tgcgcggttc cctcaggatt gacgagagag
     2821 ctcagatgtc cgttttcggg agttattcgc gttattacga cctgctctac aaggacaagg
     2881 actacggcgc cgaggccgag tacgtgcacg cgctcatttc gcagcactgc ccgggcagca
     2941 agacgatcct cgatctgggc tgcggcaccg ggcggcacgc ccagcttttc gcgaacaaag
     3001 gctacagcgt aacgggcctc gatcgctccg agcagatgct cgagacagcg cgggcggcct
     3061 cgcagggctc cgccccagag tacctcgagg gcgatttgcg cgacgcgcgc ctcggtcgag
     3121 acttcgacgt cgtcgtctcg ctttttcacg tgatgagcta ccagacgagt aacgccgatc
     3181 tgcgcggggc gttcacgacg gtgcgcgaac acctgcgccc gggcgggctc ttcttgttcg
     3241 actgctggta tgggcccgcc gtgctcacgc agcgcccgca ggttcgcatt cagcggctcg
     3301 aagacgagca cgtctcggtg acgcgactcg cagagcccac gatgcgcccg aacgagaact
     3361 tggtcgacgt caactatcac atcttcatcg aagacaagct cagccagcgc gtggaccagc
     3421 tgagcgagac gcacacgatg cgctacttgt tcgtgcccga agtcgagctc ttgttcgagt
     3481 ccgccgggtt gaagctacgc cacgcttgcg agttcatgag cgcgaaggag ccgagcctcg
     3541 acacctggaa cgtgatgttc atgggaacac gaggctgagc gtgaagctcg cggcgaaggc
     3601 cgccgcgcgg agccgcggcg agatgcgcgc atgaacggcc gccaagcgct caagtacttc
     3661 gtctacgcgg cggtgctggg cggcatcggc ttcttttttt atcgagcttt cgagaagaat
     3721 tgggacaagg tcgcggcgca ccaattcaag ctcgattacg tagcgctcgc gctcgcgctg
     3781 ttcaccttgg tcgccacgta catcgtctcg atgtacggct ggcaccgcag catcaacgcg
     3841 ctctcgcgcg gcccgcagct gaccatctcg cagagcctcg ccgcggtgaa ctccagcaac
     3901 ctcacgaagt acctgccggg aaaggtgtgg tcgtacgcct ttcagctgta ctggctgtcc
     3961 ggcgctggct tctcgaaggc gctcatcacg tacgtgaacg tcatcaacct cgcgatctcg
     4021 atcgtaacga cgctgatcgc agggttcttt ctcttgctct tcaatcccgg caagctcggc
     4081 ttcggcgcga cgttggcggc gctggtcggt gtggtggtcg cggacttggt cgcggtgaaa
     4141 ttccatgccc cgctgattcg ctttgggctc tcgcagctgg gccgcctgtt gaagcgtgag
     4201 ctcacctatt tcgagccgcc gccccgcgtg tggctcgagc tccacgccgc gcacctcgcc
     4261 gccgccttca cgttcgggct cgccgcgtat ttcacgagcc ttgccattgg ctactcgttg
     4321 ccgctttcgc aagcgcccct cgtgatggcg gcgctgctca tcgccgactt ggtggggttc
     4381 gtcgccatct tcgtgcctgg agggctcggc gtacgcgagg gtttcatgta cgtgatgctg
     4441 ggcggtgctg gcagcggctc gctggcgctc acgctgccgc tcgcgattcg catgctcaac
     4501 atggtggtcg acttgggcct cggcgggctc gcgctgcact tgtcgcgcgg cttcgctcgc
     4561 gcaaagcccg gcgctccccc gcccccacag gaaatcccga catgacagcc tctgcatcgc
     4621 gcctcgacgt cgctctgctc tgtttcccct acttcccttc gaaagacacc ggtcgcgggc
     4681 acgaccgcta cgtgtacgag ctcgccgaga acttgaagaa gctcgagcca gcgctcggcg
     4741 tgcaggtcat cgagcaaggc ttttcgaaag ggctgtcggc ggcgttcggc aagctctcga
     4801 ag
//
LOCUS       DASTKS010000300         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1163757, whole genome shotgun sequence.
ACCESSION   DASTKS010000300 DASTKS010000000
VERSION     DASTKS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163757"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFQ35_06230"
     CDS             complement(<1..1100)
                     /locus_tag="VFQ35_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9930261.1"
                     /translation="MSRRNILRGLFGVGVGLPFLESLPQRSAWAADEKPVFSLFICTV
                     LGVVPARFFPQELGPLNEAELGAAPKATAELARHAPRLLFAKNVNWVQATPTGEGHAE
                     SLVLALTARKPSSSTSNCEGTGPSADWVIANKVQPGKPALTLYAGNRKNGYIAERLAF
                     DQTGKVLAANDNPYKLYQELIGLLGPGGASTPGRETAARLLAESRKSVHDLVREELTS
                     LMGNSRLSASDKQRLQHHFDAIRDLEVKMGNLGDETALRCSQNGLELSAIQALEKFAY
                     DPHGQIEDIARLQMSLVALAFACDYNRTATLQWGDGVDKTIYDVPSNVTLGNWPFSFI
                     SHRDRSDATTGINPTAEMAHAEIDVVRMRTFAA"
     gene            complement(1133..>2536)
                     /locus_tag="VFQ35_06235"
     CDS             complement(1133..>2536)
                     /locus_tag="VFQ35_06235"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9930262.1"
                     /translation="VTDVLRFSENQQLSRDFLRPPTDAADFVNNEKLLFVDSSNLLDF
                     ETGAEAAAALATGSDAALAAVYGGTDAAGFVRTVGRRALRRPLTPAEETAYGAIFTRG
                     EELYGSGFAHGASLTIRALLESPYFLYRTELGPAGEALSGYEVASKLSFFLLDTTPSD
                     ALLDAAAAGELDTAQGLEHWARQMLDAPGAIAVMRDFHRQLFALDRAATIEKSGVTEF
                     DVALLPELAAASTAFFDQIFVGNFGLREILTAKQAYVGAGLAQFYGMAAPASGLEVRE
                     LGASRSGYFMQVPFLMQFARGAESAPSRRGFWVQRMLCGPRPGPDVVATSPPQGNRTT
                     REYMTELTAGCGGSCHAVMDPLGFAFENFDGLGREREQDNGRPVDTAGRYTFAEGVRD
                     FADGNELVRIMADSMQAHTCYSKYVAGYALARDLSPEDRPLLEGLGKVSHEHSLRELV
                     VSLVRDPAFRSRAQVRP"
BASE COUNT          453 a          817 c          819 g          447 t
ORIGIN      
        1 gcggcaaaag tgcgcatgcg aaccacatcg atctcggcgt gggccatctc cgcagtcggg
       61 ttgataccgg tggtggcgtc gcttcgatcg cgatggctga tgaagctgaa tggccaattg
      121 ccgagcgtta cgttcgacgg cacgtcatag atggtcttgt cgacgccatc tccccattgg
      181 agggtcgccg tgcggttgta gtcacaagcg aacgcgagcg cgacgaggct catctgcaag
      241 cgtgcgatgt cttcgatctg accgtgtggg tcgtaggcga acttctcgag cgcttgaatc
      301 gcgctgagct cgagcccgtt ttggctacag cgcagggcgg tctcgtctcc caggttgccc
      361 atcttcactt cgaggtcgcg aatggcgtcg aaatggtgct gcagtcgctg tttgtcggat
      421 gcgctgaggc gcgagttgcc catcaagctc gtgagctcct cgcgcaccag atcgtgaaca
      481 ctcttgcggc tctctgcgag gagtctggcc gccgtttccc tgccgggagt cgaagccccg
      541 ccgggcccga ggagcccaat gagctcttga tagagcttgt atgggttgtc gttggccgcc
      601 agcactttgc ccgtctgatc gaaggcgagg cgctcggcaa tgtagccgtt tttgcgattg
      661 ccggcgtaga gcgtgagcgc tggcttcccc ggttgaacct tgttcgcgat gacccaatca
      721 gccgacggac ccgtcccctc gcagttggaa gtcgaggaag acggcttcct ggctgtcagc
      781 gctagaacga ggctttcggc gtgcccctcg ccagtgggcg tggcttgcac ccagttcacg
      841 ttcttcgcga agagcagccg cggtgcgtgg cgggcgagtt cggctgtagc cttgggagcc
      901 gcgccgagct cggcctcatt caagggcccg agctcctgcg gaaaaaagcg cgccggaacg
      961 acgcccagga ccgtgcaaat gaacagactg aacacgggct tctcatccgc ggcccaggcc
     1021 gaacgctgcg gcaagctctc gaggaacggt aaaccgacgc ccacgccgaa cagcccgcgc
     1081 aagatattgc ggcgactcac gcggggtcgt gcccaaccga ctaaacgccc gctcatggcc
     1141 tcacctgcgc gcgcgaacga aaggcgggat cgcgcaccaa cgagaccacg agctctctga
     1201 gcgaatgctc gtggctcacc ttgccgaggc cttcgagcag tgggcgatct tccggcgaaa
     1261 ggtcgcgcgc caatgcatag ccggcgacgt acttcgaata acaagtgtgg gcctgcatgc
     1321 tgtccgccat gatgcgtacg agctcgtttc cgtctgcgaa atcacgaacg ccttccgcga
     1381 acgtgtacct gccagccgta tcgacgggcc tgccgttgtc ttgttcacgt tcgcgaccca
     1441 acccgtcgaa gttctcgaac gcaaagccga gcggatccat tacggcgtga cagcttcccc
     1501 cgcagcctgc cgtgagctcc gtcatgtact cgcgcgtcgt gcgattccct tgcggtgggc
     1561 tcgtcgcgac cacgtccggt cccggtcgag gaccgcacaa catgcgctga acccaaaagc
     1621 cacgccggct cggcgcggac tcggcgcccc gcgcgaactg catcaggaac ggcacctgca
     1681 tgaagtagcc actgcgcgag gcgccaagtt cccgcacctc gagaccactc gcaggcgccg
     1741 ccattccgta gaactgagcc agccccgccc cgacgtacgc ctgcttggcg gtaaggatct
     1801 cgcggagccc gaaattgccg acgaagatct ggtcgaagaa cgccgtcgac gcggcggcga
     1861 gctccggaag caacgcaacg tcgaattcgg tgacgccgct tttctcgatc gtggccgccc
     1921 tgtcgagggc gaagagctgc cggtggaaat cgcgcatgac cgcgatcgcg cctggcgcgt
     1981 ccagcatctg acgcgcccag tgctcgagcc cttgcgccgt atcgagctcc cccgccgcgg
     2041 ccgcgtcgag gagggcgtcg ctcggcgtcg tgtcgagcag aaagaacgaa agcttggacg
     2101 cgacctcgta gccgctcagc gcttcgcccg cgggaccaag ctcggtgcgg taaaggaaat
     2161 acggcgactc tagcaacgct cggatggtga gcgaggcgcc gtgcgcgaaa ccgctcccgt
     2221 agagctcctc cccgcgcgtg aagatcgcgc cgtaggctgt ttcttcggcg ggggtgagcg
     2281 gccgacggag ggctcggcgc ccaacggttc gcacgaaacc ggcggcgtcc gtgccgccgt
     2341 aaactgcagc gagtgccgca tcggagccag tagcgagagc ggccgccgcc tccgcgccgg
     2401 tctcgaaatc gaggagattg ctcgagtcga cgaagagtag cttctcgttg ttgacgaagt
     2461 cggcagcgtc ggtcggcggg cgcagaaagt ctcgcgacag ttgctggttc tccgaaaagc
     2521 gcagcacgtc cgtgac
//
LOCUS       DASTKS010000301         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1833358, whole genome shotgun sequence.
ACCESSION   DASTKS010000301 DASTKS010000000
VERSION     DASTKS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833358"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1063
                     /locus_tag="VFQ35_06240"
     CDS             104..1063
                     /locus_tag="VFQ35_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9930263.1"
                     /translation="MLLSIQHKTELSYAEPISESVMELRMAPRNDGQQTVRTFGLAVG
                     PEAPVFEHVDWQGNRAHHFSIVDVHDRVVIVAQSLVDTHVRRLRLEELSDTLPISGLG
                     HRYQDFLLSHGPVQRDPRLSQFAEQVGILTEKRASVVLLAVTERLRELVSYKKGVTTS
                     NTSVADVLDLGKGVCQDYAHVALAILRMLGVPSRYVSGYLFKPRSAELETHAWVEAFV
                     PSAGWVGLDPTHGDLASDSHVAVAVGRSYADVPPNRGVYRGGAEERIDVSVRIERVDE
                     EVPVEAIRRPEAPILNGVVVRERPVDLHALEHQQTQQQQQQQQ"
     gene            1294..>3818
                     /locus_tag="VFQ35_06245"
     CDS             1294..>3818
                     /locus_tag="VFQ35_06245"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9930264.1"
                     /translation="MTVRVALRHKTRYKYDRRVQLGPQVVRLRPAPHARTHVVSYAFH
                     VLPGGHFLNWQQDPQGNFLARLVFPEPVEFLDVDVELLADLKSINPFDFFLEPSAEKL
                     PFTYEPGSLRELSPYLEKAPGGPLLDAFVRELKPNGMRTVDFLVELNQLIHTKTKYLI
                     RLEPGVQAPEQTLELGSGSCRDSAWLQVQVLRRLGLAARFASGYLIQLKPDQKPLEGP
                     EGPGADFTDLHAWAEVYLPGAGWIGLDATSGLFAGEGHIPLACTPDPSSAAPISGELD
                     ECEVSFEHHMHVERVLETPRVTLPYTEEVWKRIEALGHQVDRELEKNDVRLTMGGEPT
                     FVSIDDPDGFEWNFGALGTGKRRLAGVLFRKLAQRFSHDPLLHFGQGKWYPGEQLPRW
                     ALGCYFRKDGDSIWQTKELIAEDDRDYGVTDEHAGRFIKKLAARLDVDEKHAQAAYED
                     PFYYMLKERRLPVNVTPLDNRLDDELERKRVSRIFEQGLGKIIGYALPITPLGGGAWR
                     SGPWFLRRELLYLLPGDSPMGYRLPVDSLPWVVEEDYPYVIERDPFAERSALPPYRRA
                     APELRRQRPLADGEAALRPKPLESDAQSVRTALCIEPREGVLHVFMPPVALLEDYLDL
                     VQAIESVAEELNQPVRIEGYTPPSDFRLEKFTITPDPGVIEVNIHPQGNWSDLVNTTT
                     ALYEEARESRLATEKFMLDGRHSGTGGGNHITLGGATSRESPLLRRPDLLASLVGYFH
                     DHPALSYLFSGLFIGPTSQAPRVDEARHEATHELEIAVSEARRIARESGFIAPWLVDR
                     IFRNLLADVTGNTHRTEFCIDKLYSPDSSSGRLGLLEMRSFEMP"
BASE COUNT          668 a         1278 c         1196 g          676 t
ORIGIN      
        1 cgccagacaa tggcttttta cgcgacgtcg tcgcggacgt gaacacgggc tcgatttgcc
       61 tgcaaaagac gttctttctg tgctgacccg aggagactcc tgaatgctgc tgtcgattca
      121 acacaagacc gagctcagct acgccgaacc catcagcgag tcggtgatgg agcttcgcat
      181 ggcgccgcgc aacgacggtc agcagacggt gcgcacgttc ggtctcgcgg tcgggcccga
      241 agcccccgta ttcgagcacg tcgactggca gggcaaccgc gcgcaccact tctcgattgt
      301 ggacgtgcac gaccgcgtgg tcatcgtcgc gcagtcgctg gtcgacacgc acgtgcggcg
      361 cctgcggctc gaagagctct ccgacacgct gccgatttcg ggcctcggtc atcgctacca
      421 ggacttcctg ctctcccacg ggccggtcca gcgcgacccg cggctcagtc agttcgccga
      481 gcaggtcggg atactgaccg agaaacgcgc ttcggtcgtg ctgctcgcgg tgaccgagcg
      541 gctgcgtgaa ctcgtcagct acaagaaggg cgtcaccacc agcaacacgt cggtggccga
      601 cgttctcgat ctgggcaaag gcgtttgcca agactacgcg cacgtcgcgc tcgcgatctt
      661 gcgcatgttg ggcgtgccgt cgcgctacgt gtccggatac ctgttcaagc cgcgctccgc
      721 cgagctcgag acgcacgcgt gggtagaggc gttcgtaccg tcggcgggct gggttggcct
      781 ggaccccacg cacggcgacc tcgcgtcgga tagtcacgtc gcggtcgcgg tcggccgcag
      841 ttacgccgac gtaccgccga accgcggggt ctatcgcggc ggcgccgagg agcgcatcga
      901 cgtcagcgtt cgcatcgagc gcgtcgacga agaggtgccc gtcgaggcga tacgccgtcc
      961 ggaagctccg attttgaacg gggtcgtcgt acgcgaacgc cccgtcgatc tgcacgcgct
     1021 ggaacaccag cagacacagc aacagcagca acagcagcag taatcctccg ccgtttcttg
     1081 gtcatcaggg gcaaaggccc cgctcaatcg agtcaatcga ttggtcccct tggtttttga
     1141 ggtcccgtgt ttcacggaag ctcgtagtgc cccgcacgcg cggctcgttg acgcgcgtcg
     1201 tttccccgcc cggcctttct cgcgagacag ggtttgccgg atgcccgcgc cttcgaacta
     1261 ggcgccgaaa caaatcagaa ctggagaaga gcagtgaccg ttcgcgtagc tctgcgtcac
     1321 aagacccgct acaagtacga ccgacgcgtc caactcggcc cgcaagtcgt gcgtttgcgc
     1381 cccgcgcccc acgcacgcac tcacgtagtc agctacgcct ttcatgtgtt gccgggcgga
     1441 cacttcctga attggcagca ggatccgcag gggaacttcc tcgcgcggtt ggtgttcccc
     1501 gagccggtgg agttcctcga cgtggacgtc gagctgctcg cggacttgaa gtcgatcaac
     1561 ccgttcgact tcttcctcga gccctccgcc gagaagctgc cgttcaccta cgagcctggc
     1621 agcttgcgcg agctttctcc atatctcgaa aaagctcccg gcggaccttt gctcgacgct
     1681 ttcgtgcgcg agctcaagcc gaacggaatg cgcacggtcg acttcctggt cgagctgaac
     1741 cagctcatcc acacgaagac caagtacctc atccggctcg aaccgggcgt gcaagccccc
     1801 gaacaaaccc tcgagctcgg gagcggctcc tgccgcgatt cggcatggct tcaggtgcag
     1861 gtgcttcgcc ggctcggcct cgccgcgcgc tttgcctccg gctacttgat ccagctcaag
     1921 cccgaccaga agccactcga aggtccagaa gggccgggcg ccgacttcac cgacttgcac
     1981 gcctgggccg aggtttacct gccgggagcc ggctggatcg gtctcgacgc gacgagcggc
     2041 ttgttcgccg gtgaggggca catccctctt gcctgcacgc cggatccttc gagcgctgcg
     2101 ccgatcagcg gcgagctcga cgagtgtgag gtctctttcg agcatcacat gcacgtcgag
     2161 cgcgtgctcg agacaccgcg ggtcacgctg ccttacacag aagaggtgtg gaagcggatc
     2221 gaagcgctcg gtcatcaagt cgaccgtgag ctcgaaaaga acgacgtgcg cctgacgatg
     2281 ggcggcgaac cgaccttcgt ctcgatagac gatcccgacg ggttcgagtg gaacttcggc
     2341 gcgctcggca ccggaaagcg acggctcgcg ggggtgctct tccgcaagct ggcgcagcgc
     2401 ttttcgcacg accccttgct ccacttcggt caaggcaagt ggtaccccgg cgaacaactg
     2461 ccgcgctggg cgctcggttg ctacttccgc aaggacggcg attcaatctg gcagaccaag
     2521 gagctcatcg ccgaggacga tagggactac ggcgtgaccg acgagcacgc ggggcgcttc
     2581 atcaagaagc tcgccgcgcg cctcgacgtg gacgaaaagc acgctcaggc ggcgtacgag
     2641 gacccctttt actacatgct caaagagcgg cgcttgcccg tcaacgtgac gccgctcgac
     2701 aatcgcctgg acgacgagct cgagcgcaag cgtgtctcgc gcatcttcga gcagggcctc
     2761 ggcaagatca tcgggtacgc gctgccgatc actccgctcg gcgggggggc gtggcgctcg
     2821 gggccgtggt tcttgcggcg tgagctcctc tatctgttgc ccggcgactc gccgatgggc
     2881 taccgcttgc ccgtcgactc cctgccgtgg gtggtcgagg aagattatcc ttacgtcatc
     2941 gagcgcgatc cgttcgcgga gcgctccgcg ttgccgccgt atcgccgcgc ggcaccggag
     3001 ctccgccgcc agcgtccgct cgccgacggt gaagcagcgt tgcgaccgaa gcccctcgaa
     3061 tcggacgctc agagcgtgcg cacggcgctt tgcatcgagc cgcgcgaggg cgtgctgcac
     3121 gtcttcatgc cgccggtcgc gttgttggag gactacctcg acctagtgca agcgatcgag
     3181 agcgtcgccg aagagctgaa tcaaccggtg cgcatcgagg ggtacacgcc gccctccgac
     3241 ttccgcctag agaagttcac catcacgccc gacccgggtg tcatcgaggt gaacattcac
     3301 ccgcaaggca actggagcga tctggtgaac accaccaccg cgctctacga ggaagctcgc
     3361 gagagccggc tcgcgaccga aaagttcatg ctcgacgggc gacacagcgg caccggtggc
     3421 ggcaatcaca tcacactcgg cggggccacc tcgcgcgaga gcccgctgct gcgacggccg
     3481 gatttgctcg ccagcctggt cggctatttt cacgaccacc cggcgctctc gtacctgttt
     3541 tctggcttat tcatcggtcc gacgagccaa gcgccgcgcg tcgacgaggc gcgccatgag
     3601 gccactcacg agctcgaaat cgcggtctcg gaagcgcgtc gcatcgcgcg ggaatccggc
     3661 ttcatcgcgc cttggctcgt ggatcgcatc ttccgaaacc tcttggcgga cgtgacgggc
     3721 aacacgcacc gcaccgagtt ctgcatcgac aagctctatt cgccggactc ttcgagtggg
     3781 cgcctcggcc tgctcgagat gcgaagcttc gagatgcc
//
LOCUS       DASTKS010000302        10654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1610588, whole genome shotgun sequence.
ACCESSION   DASTKS010000302 DASTKS010000000
VERSION     DASTKS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610588"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..802
                     /locus_tag="VFQ35_06250"
     CDS             302..802
                     /locus_tag="VFQ35_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9930265.1"
                     /translation="MTQAPTKPSSGRAPSQRTALGDAFSLLVVRIFQTNGYLLAAGDS
                     LAAPAGQSSARWQVMAAIEESARSVADIARLLGLARQSVQRVADLLVEDGLAVYRDNP
                     AHQRAKLLELTPHGREQLARIAEAQAVWANDLAERLGERHIANATAALTALLGLLAPE
                     VASEDS"
     gene            953..2566
                     /locus_tag="VFQ35_06255"
     CDS             953..2566
                     /locus_tag="VFQ35_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9930266.1"
                     /translation="MATIQVQDVTKAYGQKRLFQEVTVGFSAGRRYGITGPNGAGKST
                     FLKILSGELEPDTGTVQRPAKTSVLKQDQFAYESWRVLDVVVMGNSRLWSALQAKEGL
                     LAKRELTDEDGLALGELEGTIAEEDGYTAESDAGELLSGLGVPESDHEKCMSELPGGR
                     KLRVLLAQALFGNPEALLLDEPTNNLDIDSIRWLERFLQNYRGVLLVISHDRRFLNQI
                     CTHIADIDYETIITYTGGYDDMVEVKGQTRGKIEQENAERKKKIEQLQDFVARFGAGT
                     RASQVQSRKKQIDRLSLTDLKRSNIERPFIQFLVKKPSSKQTLTIDGIDKRWPEVTVC
                     ERFRAVVTKGEKVAIIGRSGVGKTTLCKMLMQELQPDAGRIEWGSGVSLGYLAQDHRE
                     GIRPGMTVTEWLHSFQPSANLQEIRGLLGRMLFKGEEGMKPTEALSGGEAVRLIFSKL
                     MLTKDNVLVLDEPTNHLDLESIIALGDALQKYEGTAFVVTHDRDLVSTFATRLFAFTE
                     SGLVDWQGNYEDFCERFGDTAFSNARKQG"
     gene            complement(2603..3058)
                     /locus_tag="VFQ35_06260"
     CDS             complement(2603..3058)
                     /locus_tag="VFQ35_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930267.1"
                     /translation="MMRFRRALLGVAVVWGSFAACGPTEPDGEDYSATRLLDRACANE
                     ACSLRGDVRKIQGLTSDSLAYELGPGAATLSIPVQSELEPLEANWGALALVKGHGTLG
                     GFLLKKDYSWQSIALDVKTGTAHVDVTEGSQVTVADVRIVRHYPTPNCE"
     gene            complement(3055..3588)
                     /locus_tag="VFQ35_06265"
     CDS             complement(3055..3588)
                     /locus_tag="VFQ35_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930268.1"
                     /translation="MNASRDGLPWRRWLGRVTLGGGYPIPSRQRSALELEGYLSPRWL
                     LGVEGGPYVGEQLVIAAFALTSLRYDSSSFGGPTLREDVNAFGLSVPFVVPLRVDAIA
                     LLTPRLGIGFGSQSFGSKHEYSTGPAWGFDACVLMQRAHFGFGVGFWSLRAPARGAAE
                     SNDYGSLLALLNFGVGR"
     gene            complement(3654..4490)
                     /locus_tag="VFQ35_06270"
     CDS             complement(3654..4490)
                     /locus_tag="VFQ35_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930269.1"
                     /translation="MGRTERASDLLSQRAIVWGLASACALAGPAGCGRKASTTAAASA
                     APSATQTASAQLFAIESVGSKATFSMQAPLERIRGEAVDAISGEVTLNLDDIEEASGR
                     VEVDLDKLVLFEQKRKDEKHEFSESAKNEQQNREAREWLQLEARPNEIAEEQAKRNRF
                     PKFLLDRLEDVSASKVSALSGDERNVGAKARGSLLLHGRTNKTNARLAFTFNYANNRV
                     QSILVKTAEPLPIALEDYDVHPRNRAGKLVKTIQEALASDYAKTLEPRVLVELSFVAK
                     PR"
     gene            4609..5529
                     /locus_tag="VFQ35_06275"
     CDS             4609..5529
                     /locus_tag="VFQ35_06275"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HET9930270.1"
                     /translation="MRKPASLLGRQLASVALFGFTALFASSSHAHFVLKSPAAWATQD
                     ALGSPQKSAPCGQSDPGAPATATGTVTAFRPGETITITIDETVFHPGHYRVLLATKSR
                     SDLPADPPVTAGDTPCGSTAISSAPVFPLLVDGALKHTAPFAGPQSIEVQLPSDVTCD
                     KCTLQVVEFMSDHGLNNPGGCFYHHCADISIQPQGGQSGGAGAPSAGASGQGSNAASG
                     GGANAGEGSGGRTSGGAANEGGRTAVGGATVVAGGPSGGGLVGLAGSDAGGQPSDVGC
                     SCSTPGGGSVPALAILSALAGIALFRARRR"
     gene            5682..6257
                     /locus_tag="VFQ35_06280"
     CDS             5682..6257
                     /locus_tag="VFQ35_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9930271.1"
                     /translation="MTVMAERGYDGASISDVAKAAHLTPGLVHYHFRNKLEILLAVVT
                     ELGDVHLATLDAALSRVQGDALAELDAFIDVHLATGKTADEKRLACWIAIGAEAIREP
                     AVRAPYQAILERMTSTVEDIARRGIRSGAFQVEAPRAVAVGIVATIQGYFGIAGAARS
                     LIPAHSAAPALKSMARGLFGVGATRRSGRRA"
     gene            6254..7468
                     /locus_tag="VFQ35_06285"
     CDS             6254..7468
                     /locus_tag="VFQ35_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9930272.1"
                     /translation="MTSPRSRSWFTFGLLGTLYAAQGLPFGFFSQALPVFLRKQGHSL
                     EAIGFSSFLAAPWAFKFLWAPLVDRHGSRRFGRRRSWIVPLQALSAVLLFALGALSST
                     VPLSLLLVAVFLTNLFAATQDIATDALALDVLPTERRGFANGIQVAGYRVGMITGGGA
                     ILLVFDALGWSASFAVMGAVMLLLSGPVLLLSEPEPTASPSQSPLGGHFLRRKDALPI
                     LSLLVTYKFGDAFALSMLRPMLVDQGLRLARIGSLLGVYGFGAGLIGALLGGALVNRV
                     GRKRALVTFGALQAVSVAGYAVLASGDMPLSMLYALTAFEHLAGGMATAALFTSMMDW
                     CRPEHNATDYAVQVSVVVVASAVAAAASGYSAHALGYAKHFWFSAALSILVLTLVNRV
                     FSRVERMGETAS"
     gene            7465..7962
                     /gene="coaD"
                     /locus_tag="VFQ35_06290"
     CDS             7465..7962
                     /gene="coaD"
                     /locus_tag="VFQ35_06290"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9930273.1"
                     /translation="MRIALYAGSFDPVTLGHLSVIRRASELFDRLWVVIGENPTKTPY
                     FSLEERLAFLRDASAGCPNVEVSFTRGFVVDYAKELGARVLVRGVRGATDADYEAELA
                     AANRSLAPAIHTVFLPADPELSKLSSSRLKELASRGMDVSAYTTPLVASALVAAFGQE
                     KSSHV"
     gene            7955..8701
                     /locus_tag="VFQ35_06295"
     CDS             7955..8701
                     /locus_tag="VFQ35_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9930274.1"
                     /translation="MSEFRVTALGVGDTFSARHFPTCLLLSASGFNLAIDCPDSYRRV
                     LASARQKALQVPELTDIDHVLITHVHGDHMNGLEGVAFFKRFVERKRVELVASPEVRA
                     VIWDQRLRAPMERLFDGAEFKSLEFGDYFSYQELGWRDARELGPFRIRARPTLHHVPT
                     SALLIEAHGKVLGYSSDTAFDPGLIEFLEPADLVLHETNYGPAHTPFAKLCGLPESLQ
                     RRMRVIHYPDEFAPGDAVLRPIEEGESLVL"
     gene            complement(8794..10029)
                     /locus_tag="VFQ35_06300"
     CDS             complement(8794..10029)
                     /locus_tag="VFQ35_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9930275.1"
                     /translation="MKRTILSASILLSLGVPLAAFGCSSSDSQKSDNDTTPIDLGSSG
                     SGSIIPGNGSLNGGMVPLTKAQVDSIKGASCVGQAVEGESLPSVLELVIDVSSSMKDP
                     APGTNRSKWEVTRDALINAVPGPASGGGLSSSIGVGLLFYPNTKSTANPAPVDITSCV
                     NTSAIVPPAQLGAANAAQRTKVRDAIQQAVLQQSTPTHDAYKYAFENGIQKTKLPGKK
                     FMLLITDGTPTLSLGCVNTTGSFNGVDPEPIVAQIQTAANAGIKTFLIGSPGSESNRN
                     WMSRAARIGGTALPNCSDNGPNYCHMDMTTAPNFSDELAKGLSSIVGAITPCNYTFPP
                     PPSGQTIDANLINVILTSSSGSQLAVRDDQGDCTQGWKLTSGNEIQLCADTCKAVQQD
                     QSISIDVVFGCSSLAQPPK"
BASE COUNT         1789 a         3434 c         3479 g         1952 t
ORIGIN      
        1 gatgcaggtg gccgtgatct cccacgtgcc tacgatgttg ccgccgcaag gcagcacctt
       61 tccgcagctc cccccggagc cctccgcatt gcctccgcaa gctgcggccg agactaggcc
      121 aacgaaaacg agcgccccaa acgagaaccg ccgctgcatc tcttcagttt tccgcgtctg
      181 gctacctcgc gcaaccgctc gaacgacacg ccgtgggctt ggtgaggctc cgcgcattcg
      241 gccgcatctt cagcgccgat cgagtgttgc gaacgcgcgg aaggagcggc actttagcgg
      301 gatgacccag gcgccaacga agccgtcttc gggcagggcg ccgagccagc gaaccgcgct
      361 cggagatgcc ttcagtttgc tcgtagtgcg catctttcag accaatggtt atttgctcgc
      421 ggcgggggac tcactcgccg cgcccgcggg gcagagcagc gcgcgctggc aggtgatggc
      481 cgcgatcgaa gagagcgctc gctcggtggc cgatatcgcc cgcttgctcg gtctcgcgcg
      541 ccaaagcgtc cagcgcgtcg ccgacttgct cgtggaagac gggctcgccg tctatcgtga
      601 caacccagcg caccagcgcg ccaagctgct cgagctgacg ccccatgggc gcgagcagct
      661 cgcacgcatc gccgaagcgc aagccgtgtg ggcgaacgat ctggctgagc gcttgggcga
      721 gcgtcacata gcgaacgcaa ccgccgcgct cacggcgctg ctcggtctac tcgcgcccga
      781 ggtggcgagc gaggactcct gagcgttcgg gacgcgagcg ccgtcgagct cgcgcgccat
      841 tgcggcgcgg tgccgtcaga cctcgcccaa gggcgaaaat ccgctcgaga accgggccga
      901 gtcgcggccc gcgctgcttt tcgcccaaaa cctgctaaag cccgccctct tcatggcaac
      961 catccaggta caggacgtca ccaaggccta cggccagaag cgcctttttc aggaagtcac
     1021 ggtcggcttc agcgcgggcc ggcgctacgg catcaccggg cccaacggcg ccggtaagtc
     1081 gacgttcctc aagatcctct ccggtgagct cgaacccgac accgggaccg tacagcgacc
     1141 ggcgaagact tcggtcctga agcaggatca gttcgcctac gagtcgtggc gtgtgctcga
     1201 cgtcgtcgtg atgggcaaca gccgcctgtg gagcgcgctc caggccaaag agggcctgct
     1261 cgccaaacgt gagctgacgg acgaagacgg acttgcgctc ggtgagctcg agggaaccat
     1321 cgcggaggaa gacggctaca cggccgagtc cgacgcgggc gagttgctct ccggtctcgg
     1381 cgttcccgag agcgaccacg aaaaatgcat gagcgagctg ccgggtggcc gcaagctacg
     1441 cgtcttgctc gcgcaggcct tgttcggaaa ccccgaagcg ttgctgctcg acgagcccac
     1501 gaacaacctc gatatcgaca gcattcgctg gctcgagcgc ttcttgcaga actatcgcgg
     1561 cgtgctcctc gtcatttcgc acgatcgccg cttcttgaac cagatctgca cgcacatcgc
     1621 cgacatcgat tacgagacga tcatcaccta caccggcggc tacgacgaca tggtggaggt
     1681 aaagggccag acgcgcggca agatcgagca agagaacgcg gagcgaaaga agaagatcga
     1741 gcagctgcaa gatttcgtcg cgcgcttcgg cgctggtacg cgcgcgtcgc aggtgcagag
     1801 ccggaagaag cagatcgacc gcctgagcct gaccgacttg aagcgttcga acatcgagcg
     1861 gcctttcatt cagtttttgg tgaagaagcc gtcgagcaag cagacgctca cgatcgacgg
     1921 catcgacaag cgctggcccg aagtcacggt gtgcgagcgc tttcgagcgg tggtgaccaa
     1981 gggcgagaag gtcgccatca tcgggcgcag cggcgtcggc aagacgacgc tttgcaagat
     2041 gctcatgcag gagcttcagc ccgacgcggg acgcatcgag tgggggagcg gcgtgagcct
     2101 cggctatctg gcgcaagatc accgcgaggg tattcggccc ggtatgacgg tgacggagtg
     2161 gctgcattcg ttccagccga gcgcgaacct ccaagagatc cgcggcctgc tcggtcgcat
     2221 gctcttcaag ggcgaggaag gcatgaagcc gaccgaggcg ctgtccggcg gtgaagcggt
     2281 gcgcctcatc ttttcgaagc tgatgctcac gaaggacaac gtgctcgtgc tcgacgagcc
     2341 cacgaatcac ctcgacctcg agtcgatcat cgcgctcggc gacgcgctgc aaaagtacga
     2401 gggcaccgcc ttcgtcgtga cgcacgaccg cgacctcgtc agcaccttcg cgacgcggct
     2461 gttcgcgttc accgagagcg gcctcgtcga ctggcagggc aactacgaag acttctgcga
     2521 gcgcttcggc gacaccgcct tcagcaacgc gcgcaagcaa ggttagtcga cgagcgggcg
     2581 ggctcgctcg ccggggaggc cctcattcgc agttgggggt cgggtaatgg cgcacgatcc
     2641 gcacgtccgc gaccgtaacc tgggagccct cggtgacgtc gacgtgcgcc gttcccgttt
     2701 tcacgtcgag cgcgatggat tgccacgagt agtctttctt cagcaggaac ccgccgaggg
     2761 tcccatgtcc cttcaccaac gcgagcgctc cccaattggc ttccaagggc tcaagctcgc
     2821 tctgaacggg aatgctgagc gtcgctgcgc ccgggccgag ctcgtaagcg agcgagtccg
     2881 acgtcaagcc ttggatctta cgaacgtcgc cccgaagcga gcaggcttcg ttcgcgcatg
     2941 cccggtcgag caagcgcgtg gccgagtagt cttcgccgtc gggctcggtg gggccgcacg
     3001 ccgcgaagct cccccaaacg accgcgacac ccaaaagcgc tcggcgaaag cgcatcaccg
     3061 gccgactccg aaattcagca gcgcgagcag ggagccgtag tcgttcgact cggctgcacc
     3121 ccgagccggc gctcgcaagg accagaaccc gacaccgaag ccgaagtgag ctcgctgcat
     3181 gagcacgcaa gcgtcgaatc cccacgcggg cccggtcgag tattcgtgct tgctcccgaa
     3241 cgactgcgaa ccaaagccta ttccgaggcg cggtgtgagg agcgcgatgg cgtcgacgcg
     3301 gagcggcacc acgaacggca ccgacaatcc aaacgcgttg acatcctccc gcagcgtggg
     3361 tccaccgaag ctcgaagaat cgtagcgaag tgacgtcagc gcgaacgcag cgatcacgag
     3421 ttgctcgccg acgtaggggc cgccctcgac ccccagaagc cagcgcgggc tcaagtagcc
     3481 ttcgagctcg agcgcgctcc tctgtcggct cgggatggga tagccgccgc cgagggtgac
     3541 tcggccgagc caacgtcgcc acggcaaccc gtcacggctc gcgttcaagt cgctcgggcg
     3601 aacctcggcc gggggagggg caggcgctgg agttgcgtcg aaggcgggca gggctacctc
     3661 ggtttcgcga cgaatgagag ctcgacgagc acgcgcggct cgagcgtctt cgcgtagtcg
     3721 cttgcgagcg cctcctgaat cgtcttgacg agcttgccgg cacgattgcg cggatgcaca
     3781 tcgtagtctt cgagcgcgat gggcagtggc tcagccgtct tcaccaggat cgactgaacg
     3841 cgattgttcg cgtagttgaa ggtgaaggcg agcctcgcgt tggtcttgtt cgttcgcccg
     3901 tggaggagca agctgccgcg cgccttggcg cccacgttgc gctcgtcacc gctcaaggcc
     3961 gaaaccttgc tcgcggagac gtcctccagg cggtcgagca agaacttggg gaagcggttt
     4021 cgtttggcct gctcttcggc gatttcgttg ggccgagcct cgagctgcag ccactctcgc
     4081 gcttcgcggt tctgttgctc gttcttcgcg ctctccgaga actcgtgctt ctcgtccttg
     4141 cgcttttgtt cgaagagcac gagcttgtcg aggtcgacct cgacccggcc gctcgcttcc
     4201 tcgatatcgt cgaggttcaa cgtcacctct ccggagatcg cgtcgacggc ctcaccgcgg
     4261 atccgctcga gcggcgcttg catcgagaac gtcgccttgg accccacgct ctcgattgca
     4321 aacagctgag ccgaagcggt ctgggtcgcg ctcggcgccg cgctcgcggc ggccgtcgtc
     4381 gaggccttgc gtccgcaccc ggccgggccg gccaaagcgc aagcgctcgc cagtccccag
     4441 acgatggcgc gctgggaaag aaggtcgctc gcccgttccg tacgtcccac gacatcagag
     4501 tttcgaaggg ggccgcgctt aagtcaagac acagggttgc ttgcccccag agcttgggcg
     4561 caaaatcgag aaagccgctc cgtagcggcc ggcaaaagtc gagaccccat gcgaaaaccg
     4621 gcatcgctcc tcggaaggca gctcgcttcg gtagctctct tcggcttcac cgcgttgttc
     4681 gcttcgtcct cgcacgcgca cttcgtcttg aagtcacccg cggcttgggc gacgcaggac
     4741 gcgctcggca gtcctcaaaa gagcgcccca tgtggacaat cggatccagg cgcgccggct
     4801 acggctacgg gtacggtgac ggcatttcga cccggtgaga ccatcacgat aacgatcgac
     4861 gagacggtgt ttcatcccgg gcactaccgc gtgctcctcg ctaccaagag ccgcagcgat
     4921 ctcccggcag atccacccgt gaccgcgggc gacacgccgt gcggcagtac cgcgatttcg
     4981 tcagcgccgg ttttcccgtt gctcgtagat ggagcgctga agcatacggc gccgttcgcc
     5041 ggacctcagt caatcgaggt ccagctgccc tccgacgtga catgcgacaa atgcacgttg
     5101 caggtcgtgg agttcatgtc ggatcacggg ctgaataacc cgggtgggtg tttttatcac
     5161 cactgcgccg acatctcgat tcagcctcaa ggtgggcaga gcgggggcgc aggcgctcct
     5221 tcggcaggtg cgtctggtca gggcagcaat gcggcgtcgg ggggaggcgc gaacgcgggt
     5281 gaagggagcg gaggacgaac gagcgggggc gcggcaaacg aaggtggacg aaccgcggtc
     5341 ggcggtgcga cggtcgttgc cgggggtccg agcggcggtg ggttggtcgg gctcgccggt
     5401 tcggatgccg gcggtcagcc cagcgacgtg ggttgcagct gcagcacccc cggagggggt
     5461 agtgttcctg ctctcgcaat actcagtgcg ctcgcgggga tagcgctctt ccgagcgcgt
     5521 cggcgctgac ctcgaacgct tttcgccgaa gcgcgactga aatcggcacg gagcagcttg
     5581 caggcctcgc tcgccgcagt catattaggt tggtcaacca acatagtatg gctcgcccat
     5641 cgaatacgca ggaacgccgc cgccaaatca cagaggggct catgaccgtg atggccgagc
     5701 ggggctacga cggcgcctcc atcagcgacg ttgccaaggc ggcgcacctc acgcctggcc
     5761 tcgttcacta ccactttcgt aacaagctcg agatcctgct cgcggtcgtc actgagctcg
     5821 gcgacgttca cctcgccaca ctcgacgcgg ccttgtctcg agtgcagggg gacgcgctcg
     5881 cggagctcga tgcgttcatc gacgtgcacc tcgcgacggg aaagaccgcc gacgagaagc
     5941 gactggcgtg ctggatcgcg atcggtgccg aggcgatacg cgagcccgcg gtgcgcgccc
     6001 cttaccaagc gattttggag cgcatgacga gcacggtcga agacatcgct cgccgcggga
     6061 ttcgaagcgg cgcgtttcag gtcgaagctc cgcgcgcggt cgcggtcggc atcgtcgcga
     6121 cgatccaggg ctacttcggg attgcgggcg cggcgcgctc gttgatcccc gcgcactcgg
     6181 ccgctcctgc gctgaagtcg atggcgcgcg ggctattcgg cgtcggcgct actcgtcgga
     6241 gtgggcgtcg cgcatgacct caccgcgctc acgttcttgg ttcaccttcg ggctgctcgg
     6301 cacgctctat gcggcgcaag gtctgccctt cgggttcttt tcacaagcgc tgccggtgtt
     6361 cttgcgcaag cagggccact cgctcgaggc aattggtttt tcctcgttct tggccgcgcc
     6421 gtgggccttc aagttcctct gggcgccgct cgtagaccgg catggctcgc gtcgcttcgg
     6481 ccgtcgccga agctggatag tgccgcttca agcactctcg gccgtgcttc tcttcgcgct
     6541 cggagcgctg agctcgacgg ttccgctctc gctcctgttg gtcgcggtgt tcctcaccaa
     6601 cctcttcgcg gcgacacaag acatcgcgac cgacgcgctc gcgctcgacg tgctcccgac
     6661 ggaacgccga gggttcgcga acggtattca ggtcgctggc taccgcgtcg gcatgataac
     6721 tggtggcggg gcaattctcc tcgtcttcga cgcgctcgga tggtcggcgt cgttcgcggt
     6781 gatgggagcg gtgatgctgc tgctgtccgg gcccgtgttg cttctttccg aaccggagcc
     6841 gacggcgtcg ccgagtcaga gtcctctcgg cggacacttc ctgcgtcgca aagacgcttt
     6901 gccgatcttg tcgctgctcg tcacttacaa gttcggcgac gccttcgctc tctcgatgct
     6961 gcgtcccatg ttggtcgatc aggggctgcg ccttgcgcgc atcggcagct tactcggcgt
     7021 gtacggcttt ggggcggggc tcatcggcgc gctgctcggg ggcgctctcg tcaatcgcgt
     7081 cggacgaaag cgagcgctcg tcaccttcgg cgcgctgcaa gcggtgagcg tcgcggggta
     7141 cgccgtgctg gcgagcggag acatgccgct ctcgatgctg tatgcgctca cggccttcga
     7201 gcaccttgcg ggtggcatgg ccacggctgc gctcttcacg agcatgatgg attggtgtcg
     7261 gcccgagcac aatgcgaccg actatgcagt gcaagtgagc gtcgtcgtcg ttgcgagtgc
     7321 ggttgcggcg gccgcgagcg gctacagcgc gcacgccctg ggctacgcaa agcacttctg
     7381 gttctccgcc gctctttcga tcttggtcct caccctcgtg aaccgcgtct tttcgcgggt
     7441 cgagcgaatg ggagaaaccg catcatgaga attgcgctct atgctggtag cttcgatccg
     7501 gtgacgctcg gacacctctc cgtgattcgc cgcgcgagcg agttgttcga ccggctttgg
     7561 gtcgtcatcg gcgagaaccc gaccaagacg ccgtacttct cgctcgaaga gcggctcgcg
     7621 ttcttgcgtg acgcgagcgc cggatgcccg aacgtcgagg tctccttcac ccgagggttc
     7681 gtggtcgatt acgcgaagga gctcggcgcg cgcgtcttgg tccgcggcgt gcgcggcgcg
     7741 accgacgccg actacgaagc cgagctagcg gccgcgaaca ggagcttggc ccccgccatt
     7801 cacaccgtct ttctccccgc ggacccggag ctctcgaagt tgagctcgtc gcggcttaag
     7861 gagctcgcgt cgcgggggat ggatgtctcg gcgtatacta cgccgctcgt cgcgagcgcc
     7921 ctcgtcgccg ctttcggtca ggagaaatcg agtcatgtct gagtttcggg tgacggcttt
     7981 gggagttgga gacacgtttt cggcacgcca tttcccgacg tgcttgttgc tctcggcttc
     8041 gggcttcaac ttggcgatcg actgcccgga ctcgtaccgg cgcgtcctcg cgagcgcgcg
     8101 ccaaaaagct ctccaggtcc cggagttgac ggacatcgac cacgtcctca tcacccacgt
     8161 gcacggcgac cacatgaacg gcctggaagg cgtcgcattc ttcaagcgct tcgtcgaacg
     8221 aaagcgggtg gaactcgtgg cgagccccga ggtccgcgcc gtaatctggg accaacgtct
     8281 gcgcgcgccg atggagcgcc tgttcgacgg ggcggagttc aagtcactcg agttcggcga
     8341 ctacttctcg taccaggagc tcggctggcg cgacgcgcgc gagctcggcc cgtttcgtat
     8401 tcgggcccgc ccaaccctgc accacgttcc gacctcggcg ctcttgatcg aggcgcacgg
     8461 caaggttctg ggctactcga gcgacaccgc cttcgatcca gggctcatcg agtttctcga
     8521 gccggccgac ctcgtcctcc acgagacgaa ctacggcccc gcccacacac cgtttgcgaa
     8581 gctctgcggc ttgcccgaat cgctacagcg acgtatgcgc gtcatccact acccggacga
     8641 gttcgctcca ggtgacgcgg tgctgcgacc aatagaagaa ggggagagcc tcgttctctg
     8701 agcgctctcc cctttctgga cgtggcgcgc cttttagcga gccgcttcac gcgccgaggg
     8761 gtgtccctcg ggcgcgaggc attggcgggc tcttcacttc ggcggctgcg cgaggctcga
     8821 acagccgaac accacgtcta tgctgatgga ttggtcttgc tgcacggctt tgcacgtgtc
     8881 cgcacagagt tggatttcgt ttccgctcgt cagcttccag ccctgggtgc agtcgccctg
     8941 atcgtcgcgc acggcgagct ggctacccga gctcgacgtg aggatcacgt tgatgagatt
     9001 cgcgtctatg gtttgccccg atgggggagg gggaaacgtg tagttgcagg gagtgatcgc
     9061 gccgacgata ctggagagcc ccttcgcgag ttcgtcgctg aagttcggcg ccgtggtcat
     9121 gtccatgtgg cagtagttgg gaccgttgtc gctgcagttc ggaagcgccg ttccgccgat
     9181 gcgcgccgcg cgcgacatcc agtttcggtt gctttcgctg ccgggggagc cgatgaggaa
     9241 cgtcttgatg ccggcgttcg cggcggtctg aatctgcgcg acgatgggct ccggatcgac
     9301 cccgttgaag ctaccggtcg tgttcacgca accgagagat agcgtcggcg tgccatccgt
     9361 gatgagcaac atgaacttct tgccggggag cttcgtcttt tggatgccgt tctcgaaggc
     9421 gtacttgtag gcgtcgtgcg tcggcgtgct ctgctgcagc accgcttgct ggatcgcgtc
     9481 gcgaaccttg gtgcgctgcg cggcgttggc ggcgccgagc tgcgcgggtg ggacgatcgc
     9541 gctggtgttg acacacgacg tgatgtcgac gggcgcgggg ttcgcggtgg atttcgtgtt
     9601 cggatagaag agcaggccaa ccccgatgct cgagctcaag ccgccgccgc tcgccggacc
     9661 gggcacggcg ttaatcagcg cgtcgcgtgt tacctcccac ttcgagcgat tggtgccggg
     9721 ggccggatct ttcatcgagc tactcacgtc gatcaccagc tcgagcaccg aggggaggga
     9781 ctcaccctcg acggcttgac cgacgcacga ggcgcccttg atcgaatcga cttgggcctt
     9841 cgtgagcggg accattccgc cgttcaaaga accgttgccc ggaatgatcg aaccggagcc
     9901 actgctcccc aagtcgatgg gcgtcgtgtc gttgtccgac ttctgcgagt cggagctgct
     9961 gcagccaaac gctgccagag gcacgccgag ggacagcagg atcgatgcgg aaaggattgt
    10021 tcgcttcatg gggctcgcct ttctaggttc cggcggtcgc gccagagcag gcagtccggg
    10081 gcgcgaagat tcttcaaact aagtgtcgac ggaagggggt ttggctcaaa aaaactgatt
    10141 cggccgagcc gtggatacaa cggtctgcat ccaggatgaa cgcggctgtc gcacatccag
    10201 gctggggttg ctcgagtcga aagacactat agcgctactt tcccgagctg tcgcgttcgg
    10261 ctctgcttcg aggagcggtc gcgtggacgt cgaagttcag cccaaccacg tcgaacgctc
    10321 gcagatggcc gctcttcgga gcggacctgc ggcacacacg cgcaacgcgc tctacgcaca
    10381 tcgctcgcgg gaagcaagcg gcgcgtacac gggcgacgga tggctcgagg agccttcccg
    10441 aaacagcgct ctagggggtg tcccaagttc cccgccctgc tgcgagccaa gatcctgcgg
    10501 ctgcgaagca cgccctcgtc gctccgctcc tcgaaaggct tcagccttcc tccggtgctc
    10561 actcctcggg cgcgcttctc gctcgcgttt cttggctctc gcgacgggca ggaacttggg
    10621 acacccccta gagcccgatc tcggtcggtt tgtt
//
LOCUS       DASTKS010000303         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_49751,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000303 DASTKS010000000
VERSION     DASTKS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49751"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFQ35_06305"
     CDS             complement(<1..1009)
                     /locus_tag="VFQ35_06305"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9930276.1"
                     /translation="MRGVAERALRTQREGNKVVMIVSAMAGETNRLLALAHDISPTPD
                     MREMDALASTGEQVSAALVAMTIQKLGGKARSLLGHQVRVQTDGAFTKARIHAIDDSR
                     LLQTVDAGEVAVVAGFQGVDAEGNITTLGRGGSDTTAVAIAAAIKADVCEIYTDVDGV
                     YTTDPNICPSARKIDRISYEEMLELASLGAKVLQIRSVEVAMKYGVPVHVRSSFSDRA
                     GTMVVGEEGLESVAVAGVAYDKGEAKVQLVGVTDKPGVVAKIFGMLAEKNVSVDMIIQ
                     SPSRGGDSRTDVTFTVVKTDLARVKDLIEQHASELGASVEYDPDIVKVSIVGLGMRSH
                     AG"
     gene            complement(1240..2043)
                     /locus_tag="VFQ35_06310"
     CDS             complement(1240..2043)
                     /locus_tag="VFQ35_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930277.1"
                     /translation="MSEGISTPESVLYDEAGDRYLVSNINGKPDGADNNGYITEISPD
                     GKVTKPKFIAGGEGKVKLDAPKGSAIAGGVLYVADISVVRKFDAKTGAPKGDITIPGA
                     TFLNDVAAAPDGRIFVTDTGVKAGANGFEPTGTDAVWVIDKAGKAKPLAKGKELAGPN
                     GVIAWEKGVLVNSMTSNQLYKLDEKGAQSEVTQLPEGMLDGLVNLGDGLLVSSWKAGA
                     VYRGKLGGKFEPVLTGFKDGPADIGFDTKRKRVLVPRFMGNAVEVYELK"
     gene            2326..2724
                     /locus_tag="VFQ35_06315"
     CDS             2326..2724
                     /locus_tag="VFQ35_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET9930278.1"
                     /translation="MTPLGRYVFETIVTLLLVTGLAVLVLMMARRSGMGRALGPLSLA
                     GRLVLDPRRAIYLVRVGETLYVVGASEAGLTKLGELARDAVNEAELGASAPPSFKELL
                     TRFGVRPPPRASAPGANAADQPRKEGDDAV"
     gene            2714..3388
                     /gene="sctR"
                     /locus_tag="VFQ35_06320"
     CDS             2714..3388
                     /gene="sctR"
                     /locus_tag="VFQ35_06320"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HET9930279.1"
                     /translation="MPSSGLFANPIALILILGVASLLPFAFMTLTAFVKISTVLQIVR
                     GAIGAQNVPSNLIVMALAGALTLLAMAPVGSRIASRSEPLFSPGADKDTQSWVVGLVE
                     AAREPVRGFLRGNASERERNRFYEVARAARPPAERSAVERDDLTVLVPAFVVSELARA
                     FALGFAIYLPFLILDLVVANVLLALGMQMLNPTQVSLPFKLLLFVAVDGWGLLSQTLV
                     SGYVPG"
BASE COUNT          559 a         1198 c         1133 g          648 t
ORIGIN      
        1 cgccggcatg agagcgcatg ccgagcccga cgatcgagac cttcacgata tcggggtcgt
       61 actcgaccga cgcgccgagc tcggaagcgt gttgctcgat gaggtccttc acgcgagcga
      121 ggtcggtctt caccaccgtg aaggtgacgt cggtgcgcga atcgccgccg cgcgacgggc
      181 tctggataat catgtcgacg gagacgttct tctccgcgag catgccgaag atcttggcta
      241 cgacgcccgg cttgtcggtg acgccgacga gctgaacctt ggcctcaccc ttgtcgtaag
      301 cgacgccggc gacagcgacg gactcgagcc cttcttctcc taccaccatc gtgcctgcgc
      361 ggtcgctgaa cgatgagcgg acgtgcacgg gaactccata cttcatggca acctctaccg
      421 agcgaatctg cagcaccttc gctccgagcg aggcaagctc cagcatttcc tcgtaactga
      481 ttcgatcgat ttttcgagcc gacggacaga tgttgggatc ggtcgtatag acgccgtcca
      541 cgtcggtata gatctcgcaa acgtcggctt tgatggcggc ggcgatggcg accgcggtcg
      601 tatccgaacc accgcgcccg agcgtcgtga tgttgccctc ggcgtccacg ccttggaacc
      661 ccgcgacgac ggcgacctcg ccagcgtcga ccgtttgcaa cagccgagag tcgtcgatcg
      721 cgtgaatacg cgccttggtg aaggcgccgt cggtctgcac gcggacctga tgaccgagca
      781 agctgcgggc cttgccgccg agcttctgaa tggtcatcgc gacgagcgcg gcgctgactt
      841 gctccccggt ggacgcgagc gcatccattt cgcgcatgtc cggggtgggc gagatgtcgt
      901 gggcgagagc gaggaggcga ttcgtttcgc ccgccatcgc gctcacgatc atgacgacct
      961 tgttaccctc gcgttgcgtg cggagggcgc gttccgccac tccacgcata cgttcgaggt
     1021 taccgaccga ggttccaccg aatttctgga cgacgagagc caagcgggag tgggcctacc
     1081 tccctgcttt cgcagagtca atctcgcgca acggtttccc gcgttccagc gccgccgccg
     1141 agctcagccc cggattgaac acgggatgtt cagccatcag cggctgcgcc atccggccga
     1201 tgggccgcca gaggagagcc ccggcggccc ccagcgaaat cacttcagct cgtagacttc
     1261 gaccgcgttg cccatgaacc ggggcaccaa aacgcgcttg cgcttcgtgt cgaagccgat
     1321 gtccgcgggc ccatccttga aaccagtgag gacgggctcg aacttgccgc ccaacttgcc
     1381 ccgatacacg gcgccggctt tccagctcga gacgagcagg ccgtcaccga ggttgacgag
     1441 cccatcgagc atgccttcgg ggagctgcgt gacctccgac tgcgcaccct tctcgtcgag
     1501 cttgtagagc tggttgctcg tcatcgagtt cacgagcacg cccttttccc aagcgatgac
     1561 gccgttcgga ccggcgagct ctttgccctt ggcgagcggc ttcgccttgc cggccttgtc
     1621 gattacccac accgcgtcgg tgccggtggg ctcgaagccg ttcgctccgg ctttcacgcc
     1681 ggtgtcggtc acgaagatcc ggccatcggg ggctgcggcg acgtcgttca aaaacgtcgc
     1741 gcccgggatg gtgatgtcgc ctttgggagc gccggtcttg gcgtcgaact tgcgcacgac
     1801 gctgatgtca gcgacgtaga gcacgccgcc cgcgatggcg ctgcccttcg gcgcatcgag
     1861 cttgaccttg ccctcgccgc ccgcgatgaa cttgggcttc gtgactttgc cgtcgggcga
     1921 aatctcggtg atgtagccgt tgttgtcggc gccgtcgggc ttgccattga tgttcgacac
     1981 caagtaccga tcgcccgctt cgtcgtagag cacactttcc ggcgtgctga tgccctcgct
     2041 cacccggaac accggcgtgg gacccggagc gggcgcgggc tcggcagctt tggcaggagc
     2101 aggggccggc tccggcgaaa cgctactctg aatgggcgca ggcggaagcg aagcgctggc
     2161 gggtggagcg ggcggcgcgg cttcgggcgg cggaggcgac gcacaggccg ctgcgagaag
     2221 agaccctagg gaaacactca gcaagtgacg agtcatgcgc gcatggatag ggccgctcga
     2281 gaggagacgt caaccggcgc cccaccatgg taacgtgcgc gcgctgtgac tccactcggg
     2341 cgctacgtgt tcgagaccat cgtgaccctt ttgctcgtga ctgggctcgc ggtgctcgtc
     2401 ctgatgatgg cgcgtcgaag cggcatggga cgcgcgctcg ggccgttgtc gctcgcgggc
     2461 cgcctcgtgc tcgatcctcg ccgcgccatc tatctcgtac gtgtcgggga aactctgtac
     2521 gtggtcggcg cgagtgaagc gggcctcacc aagctcggtg agctcgcgcg cgacgccgtc
     2581 aacgaagcgg agctcggcgc gtccgcgcca ccttcgttca aggagctcct gacacgcttc
     2641 ggtgtacgcc caccgccgcg tgcatccgca ccgggcgcga acgccgcgga ccaaccgcga
     2701 aaggagggcg acgatgccgt ctagtggctt gttcgcgaac cccatcgcgc tcattctcat
     2761 cctcggagtc gcatcgctgt tgcccttcgc cttcatgacg ctgaccgcgt tcgtgaagat
     2821 ctcgacggtg ctgcagatcg tgcggggcgc cattggcgct cagaacgtcc cgtcgaacct
     2881 catcgtgatg gcgctcgctg gagcgctcac gctgctcgcg atggcgcctg tcggctcgcg
     2941 catcgcttcg cgcagcgagc cgctgttttc gcccggcgcc gacaaggaca cgcagagctg
     3001 ggtcgtcggc ctcgtcgaag ccgcccgcga gcccgtgcgc ggctttctgc gcggcaacgc
     3061 gtcggagcgc gagcgcaatc gcttctacga agtcgcgcgc gcggcgcgcc cacccgccga
     3121 gcgcagcgcc gtcgaacgcg acgacctcac ggtgctcgtt cccgccttcg tcgtcagcga
     3181 actcgcgcgc gccttcgcgt tgggcttcgc aatctatctt ccatttttga ttttggacct
     3241 cgtggtcgcg aacgtgctcc tagccctcgg catgcaaatg ctgaatccga cccaagtgag
     3301 cttgccgttc aagctgctct tgttcgtcgc ggtcgacggt tggggcttgc tctcgcaaac
     3361 gctcgtgagc ggctacgtcc ctggctgacc acgccgcgag cacgcgacgt cctgccttgc
     3421 cacacccgcg agcacgaaac gtcctggctg accacgcccg cgggaacgcg acgtcctgcc
     3481 ttgccacgcc cgcgagcgcg gcacgcaacg tcccgccttg ccacacccgc gagcacgc
//
LOCUS       DASTKS010000304         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1611133, whole genome shotgun sequence.
ACCESSION   DASTKS010000304 DASTKS010000000
VERSION     DASTKS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611133"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFQ35_06325"
     CDS             <1..580
                     /locus_tag="VFQ35_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930280.1"
                     /translation="AELERVARLRQQAMYEVDVRRVLLVIELVGAGMGQNQGAPLVKK
                     GGTLILTHPCTDKFDHEQHAPYIDFVHRLLPETRDALELGSRYEQKFAKDPAFLQMFR
                     DGHAYHPAHPFFMWYWGENGRQHLGRVIVVGADNEYIPRLLGWETAPSMEEALYRAKN
                     GEARSLDITLLHMPPIVMGDVTVKPSAEGAKP"
     gene            577..>2918
                     /locus_tag="VFQ35_06330"
     CDS             577..>2918
                     /locus_tag="VFQ35_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9930281.1"
                     /translation="MSEQLTNGVTRVTAQAPREPLELTRLFTGKTLVVVGGTGFLGKV
                     WLSFMLTRFPELGRIYLVVRPKGESTAQQRFDRDIIGSEVFAPLRERYGAGFADFLRS
                     KVYPVAGDVVQPYCGLDPGLRQQIAGSVDAVVNVSGVVDFDPPLDEALEVNAFGVQNL
                     VALARDFGGVPLLHTSTCYVAGARTGFVEEVDPFEHPFPRASELDRVHWDPDREIAEC
                     LDVIEQARHRASDAFRQSRFLDEAKKNLLDRGEPARGRVLESEVQKVKRKFVEARLAE
                     MGMERSNFWGFPNTYTYTKAIGEQIASSSGLPFTIVRPAVVESTLSFPFPGWNEGINT
                     SAPLIFILRQGGLQIPGSDNFLDVIPCDMVASGLVLALGELLEGRAKAVYQLGSSDTN
                     PCTMRRFFELTGLYKRRHYQRSGKGGPLLSFLQSHFEGAMLSKDQFERIGPKVIAQKA
                     RGLSNLLSLAAQGPIAPLLKPAAEAVEKFSEQQRRVGNILEVFVPFTAQYKYVFRTDN
                     VRAAQARLAPEDKGKVDFSPERINWRDWFLDVHVPGLERWVFPNIEAKLRRPKKAAQR
                     HETLPLMLEEMAERFDLAVALQRMDAQGLSRITFRDWRNLAAATAERLHQAGIRRGDR
                     VLLAGENHPAWPIAFFGIMWVGATAVPLDANIEADIADNLARVSQARILLADARARGR
                     LEARLAERVQVMDLQDITREGEPRSLEPVSADDIAALIYTSGTTGNPKGVALSQKNLT
                     SLIGMLAPLFPLGRDDRILSVLPLHHTFELTCGMLLPLSRG"
BASE COUNT          504 a          976 c          936 g          502 t
ORIGIN      
        1 agccgagctc gagcgcgtcg cgcgtctccg gcagcaagcg atgtacgaag tcgatgtacg
       61 gcgcgtgctg ctcgtgatcg aacttgtcgg tgcagggatg ggtcaaaatc agggtgcgcc
      121 cctcgtgaag aagggcggca ccctgatttt gacccatccc tgcaccgaca agttcgatca
      181 cgagcagcac gcgccgtaca tcgacttcgt acatcgcttg ctgccggaga cgcgcgacgc
      241 gctcgagctc ggctcgcgtt acgagcagaa gttcgccaaa gacccggcgt ttttgcagat
      301 gttccgcgac gggcacgcct accaccccgc gcatccattc ttcatgtggt actggggcga
      361 gaacggccgt cagcacctcg ggcgcgtgat cgtggtcgga gccgacaacg agtacatccc
      421 gcgtctgctc ggctgggaaa ctgcgccgag catggaagag gcgctctacc gcgccaagaa
      481 tggcgaggcg cgttcgctcg acatcacgct cttgcacatg ccgccgatcg tgatgggcga
      541 cgtaaccgtc aagcccagcg cggagggagc gaagccttga gcgaacaact cacgaatgga
      601 gtgacccgcg tgaccgcgca ggcgccccgc gagccgctcg agctcacacg cctcttcacc
      661 ggcaagacgc tcgtcgtcgt gggcggcacc ggctttctgg gcaaggtgtg gctgtcgttc
      721 atgctcacgc gcttccccga gctcggacgc atctacttgg tggtgcggcc gaaaggagaa
      781 agcaccgccc agcagcgctt cgatcgggac atcatcggga gcgaagtgtt cgcgcccctg
      841 cgtgagcgct acggcgctgg cttcgcggat tttctgcgca gcaaggtgta cccggtggca
      901 ggcgacgtcg tgcaacctta ctgtgggctc gacccggggt tgcgacagca gatcgccggc
      961 tcggtcgacg cggtcgtgaa cgtgtcgggc gtagtcgatt tcgatccgcc gctcgacgag
     1021 gcgctcgagg tgaacgcctt cggggtgcag aacctggtcg cgctggcgcg cgatttcggc
     1081 ggcgtaccgc tgctgcatac gagcacttgc tacgtggccg gcgcgcgcac cggcttcgtc
     1141 gaagaggtag atccgttcga gcatcccttc cctcgcgcca gcgagctcga tcgcgtgcac
     1201 tgggatccgg atcgcgaaat cgccgagtgc ctcgacgtca tcgagcaggc gcgtcaccgg
     1261 gcgagcgacg cctttcggca gagccgcttc ctcgacgagg cgaagaagaa cctgctcgac
     1321 cgcggcgagc cagcgcgcgg gcgtgtgctc gagagcgaag ttcagaaggt gaagcgcaag
     1381 ttcgtcgagg cgcgcctggc cgaaatgggc atggagcgct ctaacttctg gggctttccc
     1441 aatacgtata cttacacgaa agccatcggc gagcagatcg cgtcgagctc ggggctaccg
     1501 ttcaccatcg ttcgacccgc ggtggtcgag tcgacgctga gcttcccctt tcccgggtgg
     1561 aacgagggga tcaacaccag cgctcctctc atcttcatct tgcgccaggg cgggctccag
     1621 atccctggct ccgacaactt cctcgacgtg attccgtgcg acatggtcgc gagcggactc
     1681 gtgctcgcgc tcggtgagtt gctcgaaggc cgcgcgaagg cggtctacca gctcgggtcg
     1741 tccgacacga acccgtgcac gatgcgccgt ttcttcgagc tcaccggcct ctacaagcgc
     1801 cgccactacc agcgctcggg caagggcggg cctttgctca gcttcttgca gtctcacttc
     1861 gagggcgcga tgctctcgaa ggatcagttc gaacgcatcg gcccgaaggt catcgcccag
     1921 aaggcgcgcg gtctttcgaa cctgctcagc ctcgccgcgc aaggcccgat cgcgccgctc
     1981 ttgaagccgg ccgcggaagc cgtcgagaag ttttcggagc agcagcgccg cgtcggcaac
     2041 atcctcgaag tgttcgtgcc gttcacggcg caatacaaat acgtcttccg caccgacaac
     2101 gtgcgcgcgg cccaggcgcg gctcgctccc gaagacaagg gcaaggtcga cttcagcccc
     2161 gagcgcatca actggcgcga ctggttcctg gatgtccacg tgccgggcct cgagcgctgg
     2221 gtgttcccca acatcgaagc caagctgcgt cgcccgaaga aggccgcgca acgtcacgag
     2281 acgctccccc tcatgctcga agagatggca gagcgcttcg accttgccgt cgcgctgcag
     2341 cgcatggatg cgcagggcct gagccgcatc accttccgcg actggcgcaa tctcgccgcc
     2401 gcgaccgccg agcggctcca ccaggccggg atccggcgtg gcgatcgagt gctcctcgca
     2461 ggcgaaaatc acccagcgtg gccgatcgcc ttcttcggca tcatgtgggt gggcgcgacc
     2521 gcggtgcccc tcgacgcgaa catcgaggca gacatcgccg acaatctggc gcgcgtctca
     2581 caagctcgca tcttgctggc cgatgcgcgg gcgcgcggtc gcttggaagc gcgcctcgcc
     2641 gaacgcgtgc aggtcatgga tctgcaggac atcactcgag agggtgaacc gcgctcgctc
     2701 gagcccgttt cggccgacga catcgcggcc ctcatttaca cgagcggaac cacggggaac
     2761 ccgaagggcg tcgcgctctc gcagaagaat ctcacgtccc tcatcggcat gctggcgccg
     2821 ctgttcccgc tcgggcgcga cgatcgcatc ctcagcgtgc tgcctctgca tcacacgttc
     2881 gagctcacct gcggcatgct gctcccgctc tcgcgcgg
//
LOCUS       DASTKS010000305         9019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1164592, whole genome shotgun sequence.
ACCESSION   DASTKS010000305 DASTKS010000000
VERSION     DASTKS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164592"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..319)
                     /locus_tag="VFQ35_06335"
     CDS             complement(161..319)
                     /locus_tag="VFQ35_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930282.1"
                     /translation="MFLRSARNTKPPTTGVRTQHLDGTRRDCVLTSDFDELERLRAHF
                     PTVRLVAV"
     gene            469..2130
                     /locus_tag="VFQ35_06340"
     CDS             469..2130
                     /locus_tag="VFQ35_06340"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9930283.1"
                     /translation="MQNTRLRCNESRLVSPSHGSMFALLLAFAAGCGASAETSDGSPE
                     FASVTQADSQVPNPPPLSRVAVPQPIGGNIVNVPAAIRLGKALFWDIQAGGDGQTACA
                     TCHAHAGADNRLTNTMNPGPNGTFESGGVTGPGQTWNGTSVAQNADDIIGSQGVVGAV
                     FSAIDANPNNAADSCTPDQTAPFLANRRVTGRNAPTVIGAVFFRDNFWDGRANHRFNG
                     INPFGDTANNVDGNFLDADNASLASQAVGPPNNPVEMSCLGRLFNGSNSLASKLLART
                     PLGKQVVSTTDSVLGALANATGTGLTTSYTQMVVEAFGAGQGANAQAEFSMFWGQAVQ
                     AYEATLIPDQTPFDKFLAGDNNAMTSNQKAGWTAFKGKGGNPTDRSCFSCHAGSELSD
                     ATVSYAAAHGTTNVDGGDQGFHNIGVRPTNDDLGRGATGPNGVDWSVSGSNFDRGAFK
                     TPGLRNVKLTGPYFHDGGVATLAEVFEFYHDHGFFENSGSLASQFDRLNTSGGDRNSI
                     VDFLTNALTDCRVEKRRAPFDGPALPLPNGAALAAVGANGTGSCP"
     gene            complement(2203..2994)
                     /locus_tag="VFQ35_06345"
     CDS             complement(2203..2994)
                     /locus_tag="VFQ35_06345"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9930284.1"
                     /translation="MVVEASTETAGSDVSDVSDVSDVSNVSVSDVSDVSDVSDVNVTS
                     EREQELLRRVASGERRAFEGIMRRYNQRLYRVARAIVGDDSEAEDAVQDAYLAAFVAV
                     PQFAGRSSVGTWLTRIAINEAFARRRARARRRTLDELELVEEVVADREGTPEEIVSMQ
                     QLVKLVESAVDELPASYRLVLVLRQVQGLSGEETAESLGLSVEAVRVRLHRARVLLRE
                     RLHTVSGVRLELELFEFAWQRCERIVARALARIERADDTLPGSPG"
     gene            3671..4480
                     /locus_tag="VFQ35_06350"
     CDS             3671..4480
                     /locus_tag="VFQ35_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9930285.1"
                     /translation="MKVLVNSAGVQIVAHVTGEGTPVLLLHGFPDSAALWCHLTPKLN
                     AAGYKTIAIDLRGFGESDAPAKVPSYDLRRAAQDALAVLDALAIQRAHLVGHDWGAAL
                     GWLLAGSRPDRFSSFSALSLGHARAYSKGGLRQFTKGWYMLPLLVPLLGEGTVRAANW
                     YLLRRANPSESERWVRNLSRPGRLTAALNWYRANALHPPEHPNVNIPVLGIWSARDLA
                     LTENQMLESAKFVDGPWRYERIEGAGHWLPLDAAERIAPILLEHFESANRR"
     gene            complement(4539..5603)
                     /locus_tag="VFQ35_06355"
     CDS             complement(4539..5603)
                     /locus_tag="VFQ35_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9930286.1"
                     /translation="MDIPRIRFASSARGKVAFQQVGDAKPTFLLVPPLAQHIEMMWEQ
                     PVFWRPIQRMASAFRFIQYDKLGTGLSDPSPGRASIDERIEELTAVLDAAHVERVWLG
                     GFSEGGVIALAAAARMPERVAGLVLISTYSGNTALGEAAAYGPVPTKSDFRELFAAVV
                     AHWGTDSTLILSDFAPSFRSIPALTRWVPRYERASASPAMIGTLMATSLTLDATPALP
                     SIEQPALVLHLAGDRVIPAAFGRMLGQRLKRARYVELAGDDHFSWVSPSIDQQIDLIF
                     EFTGVNGSGPRVSAVWEPWSVLTPSERRVVGLAQRGLTNTEIATQLDLSSRTVENHLT
                     RAYGKLGVRSRTELALLSRA"
     gene            complement(5671..7029)
                     /locus_tag="VFQ35_06360"
     CDS             complement(5671..7029)
                     /locus_tag="VFQ35_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9930287.1"
                     /translation="MTRAVVVGGSIAGLLVGRVLAEVCDEVLVVERGELPDTPLPRAG
                     VPQGHHAHGLLAGGLQALEQLLPGLCRELEERGCPTGDNLRDAAWIFSGRRLALGDSG
                     VRGMTVARPLLDHVIQSRVTRLSNVHIRTGTRVTGLLHEAGRVTGLRVASVPGDEETL
                     EADLVIDASGRHSELPNWLLALGLSAPRIEEITLPTHYASRTYARRPNHLNGAIAIAV
                     VSDPKVPRGGVALALDRERWIVSLYSMGGERPPQDAAGFAQFARTLATPVLAEVLEDS
                     EPLSETRTLRFVSSIRRHYDSMRDFPRGLLVCADALASFNPTFGQGITVAAKQALLLQ
                     QLGRRVRLSQLGTAFLESAAPIIDVAWSASAGRLFLYPGAVGRPTLKMRIANFYLPRV
                     IASAHADPEIAAALLEVLHFQAPPKRLFAPGVMLRLLAGMRERGAADPRSLLARSAPP
                     FE"
     gene            7222..8346
                     /locus_tag="VFQ35_06365"
     CDS             7222..8346
                     /locus_tag="VFQ35_06365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9930288.1"
                     /translation="MTTSIQVDTETNSSRAGAHPGAERAPDSRATERFAQRVLGDLSA
                     AMTGVMVRLGHELGLYRALGEHGPLTSGELSLRTLVLERYAREWLHQQRAAGYLDYDP
                     QSAKFRLAEGAFAVVVAEDSPVFLLPAFDIVASIWADEARLEQAFTRGEGIGWGEHDP
                     RLFSGCARFFGAAYASHLVQDWLPVLDGVTDKLGRGATVADVGCGHGISTVLMARAFP
                     ASRFVGFDSHAASIDVARQRAKQAGVCERVRFERALSTDFGDERYDLICFMDSFHDMG
                     DAVAAARHAASRLTNGGTVMLVEPLAAERPEENRGPIAAMNYAASTALCTPNALCHRH
                     GIALGAQAGPEALRQALAEGGLTHFRVAATTPFHMVIEAR"
     gene            8546..>9019
                     /locus_tag="VFQ35_06370"
     CDS             8546..>9019
                     /locus_tag="VFQ35_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930289.1"
                     /translation="MLNCLKIRDLAIVEELTLDLGPGLNVLTGETGAGKSVLVGALEL
                     VLGGKAKAELVRSGAAAGEVEALFEVGGDSPVREQLRALNLGGSDELVVRRVVSSSGR
                     ARAYVNGKLATQQMLAELTRGLADISSQHEHHTLTDPRTHLGYLDAFGGLAALCER"
BASE COUNT         1609 a         2907 c         2950 g         1553 t
ORIGIN      
        1 atcacgctgt ggtttggcgg aggcgccgaa caaagcacgt gctgaccgtg agccgagggg
       61 ctgcccgcgg agttctccaa gcgcgcgcag caccggttga gcctgtcgcg cttgacgcta
      121 gctcaccggc atgctgccta cgcgccagtg cggaaaccgc tcacacggcg acgagacgga
      181 cggtagggaa gtgcgcacgt aagcgctcga gctcgtcgaa gtcggaggtc aacacgcagt
      241 cgcgccgcgt cccgtctagg tgctgcgtcc gcaccccggt cgtcggcggc ttcgtgtttc
      301 gggctgagcg caagaacatc ctcatctcgg cgccggtcga tttctgcgag gacgtttgta
      361 acgtcgccca cgcctcgtga ctcaaagagg ggttcgcgcg gtacgtccgt gcgagaccag
      421 ggatgagtcc gagttgtggg cgtgtgacgc gcccgggagg agtattccat gcagaacacg
      481 cggcttcggt gcaacgagag tcgtctcgtt tcaccttcac atgggagtat gtttgcgttg
      541 ttgctcgcgt tcgccgctgg gtgcggggcg agtgcggaga cgagtgacgg ctctccggag
      601 ttcgcgagcg tgacgcaagc cgactcacaa gttccgaatc cgccgccgct ctcccgggta
      661 gcggtgccgc agcccattgg gggcaacatc gtcaatgtgc cagcggcaat tcgacttggg
      721 aaagcgctgt tctgggatat tcaggcgggt ggggacggac agacagcgtg cgccacgtgc
      781 cacgctcatg caggcgccga taaccgtctg accaacacga tgaatcccgg cccgaacggc
      841 accttcgaga gtggcggagt cacgggcccg gggcaaacct ggaatggaac gagcgtcgcc
      901 cagaacgccg acgatatcat cggatcgcag ggggtcgtcg gcgcggtatt cagcgcaatc
      961 gatgcgaacc cgaacaacgc cgctgacagc tgcacgcccg atcaaacggc gccgttccta
     1021 gcgaaccgcc gcgttacggg tcgcaacgca ccgaccgtga tcggggcggt gttcttccgc
     1081 gacaacttct gggacggccg cgccaaccat cgtttcaacg gcatcaatcc cttcggcgac
     1141 accgcgaaca acgtcgacgg gaacttcctc gacgccgaca acgcgagcct ggcttcgcaa
     1201 gcggtcggcc cgcccaataa tcccgtcgag atgtcctgtc ttggtcgcct cttcaacggg
     1261 agtaatagcc tcgcgagcaa actgctcgca cgaacgccgc tcggtaagca ggtcgtctcg
     1321 acgacggaca gcgtgctggg cgcgctcgcc aacgcgaccg ggaccggcct gaccacgtcg
     1381 tacacccaga tggtcgtcga agcgttcggc gccgggcagg gagcgaacgc ccaggccgag
     1441 ttctcgatgt tttggggcca ggcggtgcag gcatacgagg cgacgttgat tccggaccag
     1501 actccattcg acaagttcct cgctggtgac aacaacgcaa tgacctcgaa tcaaaaggcg
     1561 ggatggaccg catttaaagg caagggtggc aacccgacgg atcgttcgtg cttctcgtgc
     1621 catgccggct ccgagctctc cgacgcgacg gtgagctacg ccgcagctca cggcacgacc
     1681 aatgtcgacg gcggagatca ggggtttcac aacattggcg tgcgacccac gaacgacgac
     1741 ttgggccgcg gtgccacggg gccgaacggc gtcgattggt cagtcagcgg ctccaatttc
     1801 gatcgcggcg ccttcaaaac gccggggctt cgcaacgtga agctcacggg gccgtacttt
     1861 cacgacggcg gcgtcgccac gctcgcggag gtgttcgagt tctatcacga tcacggcttc
     1921 ttcgagaatt ctgggtcgct cgcgagccag ttcgaccgct tgaacaccag cggaggcgac
     1981 cgcaacagca tcgtcgactt tctcacgaac gcgctcaccg actgccgcgt ggagaagcga
     2041 cgcgctccat tcgacggccc ggcattgcct ttgccgaacg gagctgcgct cgccgcagtc
     2101 ggcgcgaacg gaacggggag ttgcccgtag actaaaggcc cgccccaaag cccgctctgc
     2161 aacggcccgc ggcgcggatt cactttcgac atgctgctcg gctcacccgg gcgaaccggg
     2221 cagcgtgtcg tcagcgcgtt cgatgcgggc gagcgcacgc gcgacgattc gctcgcaacg
     2281 ttgccacgcg aactcgaaaa gctccaactc gagccgaact cccgaaaccg tgtgcagtcg
     2341 ttcacgtagc aggacgcgcg cgcggtgcag gcggacgcgc acggcctcga cggagaggcc
     2401 gagtgactcg gcggtttctt ctccgctcaa gccttgcacc tgacggagca cgagcaccag
     2461 ccgatacgac gctggaagct cgtcgacggc agactcgacg agcttcacca gttgctgcat
     2521 cgaaacgatt tcctccggtg tcccttctcg gtcggcgact acttcctcga cgagttcgag
     2581 ctcgtcgagc gtgcgccggc gggcacgcgc ccgacgtcgc gcaaacgctt cgttgatcgc
     2641 tattcgggtg agccaagtgc ctacgctcga acggccggcg aattggggca cggctacgaa
     2701 agcagccaga tacgcatcct gcacggcgtc ttccgcttcg gagtcgtcgc cgacgatggc
     2761 ccgcgccacg cgataaagac gctgattgta acgccgcatg atgccttcga acgcgcgccg
     2821 ctccccgctt gcgactcgac gcaagagctc ctgctcgcgc tcgctagtaa cgttgacgtc
     2881 gctaacgtcg ctaacatcgc taacgtcgct aacgctaacg ttgctaacgt cgctaacgtc
     2941 gctaacgtcg ctaacgtcgc tgcctgcggt ctcagtgctt gcttccacga ccatggggca
     3001 ccccttgtca gcgcaaatga agagaagtag gcgctgtcgg ttgcgagcga agccagccgc
     3061 tctcgatgct cgcagcgtgg ccgtgcgcct tgccggtgag tcaataggac cacgcgctac
     3121 ttatcgcaag tctaattcat gcgtcgcatt gagtgcgctt gcggctccgt tgccggcgcg
     3181 cgagcaagtg ttcaatcgtt ttttcagcgc tcatggagat gcgcagtcgt tttgtcgggc
     3241 tttcgcgctc cgataagtga tcgcggcgcg cattcaaagc atgagtagtt cgttgcgaca
     3301 cgcggcgaaa tacctcgggg agccttactg aaggggtgtt gggcgtcgaa cgaacgtgac
     3361 gaagtcgcat cgtcgcggcg caattccatc ccaagtcgcc gggcgcgtcg aaagtttgga
     3421 acccgcacgc ttatcgcacg ttgatcccgc aaaacacgtc cgtccacttc atcctcgatc
     3481 tcgatccctc ctcgttctct cgatcttttt tctcaccgtc gcaaatcggt aggaaggggc
     3541 gcggtcgctg cagtctgctg tcacaccgcg cttcaagccg tcgatccgcg cactcgccat
     3601 atactgcgaa gaacattacg tagctccgtc gcgccgtccc gggcatgatg agcgtggagc
     3661 tcaccggcca atgaaagttc tcgtcaattc cgcaggcgtt cagatcgtgg ctcacgtaac
     3721 gggagagggc actcccgtgc tgctcctgca tggctttccc gactccgcgg cgctctggtg
     3781 ccacctgact ccgaagctga atgcggcggg gtacaaaaca atcgcgattg acctccgcgg
     3841 attcggcgag tcggacgcgc ccgcgaaagt cccgagctac gacctcaggc gtgcggcaca
     3901 agacgcgctt gccgtgctgg atgctctcgc aatccaacgc gctcatctcg tcggccacga
     3961 ttggggcgct gcgttggggt ggctactcgc ggggagccgt ccggaccgct tctcgagctt
     4021 ctcggctctc tcactcggac acgcgcgcgc ctattcgaag ggcgggctgc gacagttcac
     4081 gaagggctgg tacatgttgc ccctgctcgt gccgttgctc ggtgagggga ccgtgcgtgc
     4141 ggcgaattgg tacctcttgc ggcgcgcaaa ccccagcgag agcgagcgct gggttcgaaa
     4201 cctctcgcga cccggcaggc tcaccgccgc gttgaactgg tatcgcgcca acgcgctcca
     4261 cccaccagaa cacccgaacg tgaacattcc ggtgctcggc atttggagcg ctcgtgactt
     4321 ggctctgacc gaaaatcaga tgctcgagtc cgcgaagttc gtcgatgggc cgtggcgcta
     4381 cgagcgaatc gaaggtgcgg gccactggct ccctctcgac gccgccgagc gcatcgctcc
     4441 aatattgctc gagcacttcg aaagtgcgaa ccgtcgctga agactggccc cgagctacag
     4501 cgactgggga ccgacgtgcg acccgcgctg gcggcgtgtc acgcgcggct caacagagcg
     4561 agctctgtgc gggagcgaac gccgagcttg ccgtaggccc gcgtcagatg gttttcgacc
     4621 gttcgcgagg agaggtcgag ctgggttgcg atctcggtgt tcgtgaggcc gcgctgggcg
     4681 agcccgacga cgcgccgctc ggacggggtg agcaccgacc agggctccca caccgcgctg
     4741 acgcgcggac cactgccgtt tacgccggtg aactcgaaga tgagatcgat ctgctggtcg
     4801 atactcgggc tcacccagga gaagtgatcg tcgcccgcga gttcgacgta gcgcgcgcgt
     4861 ttcagtcgtt ggccgagcat gcgaccgaac gcggcgggaa tgacccgatc gcccgccagg
     4921 tgcagtacga gcgcgggctg ttcgatgctg ggcaacgccg gcgttgcgtc gagggtgagg
     4981 ctggtggcca tcaaggtgcc gatcatcgcg gggctcgccg acgcgcgctc gtagcgcgga
     5041 acccaccgcg tgagcgccgg tatcgagcga aacgacggcg cgaagtcact gaggatcagt
     5101 gtcgaatcgg taccccagtg agccacgacc gcggcgaaca attcgcgaaa gtccgacttg
     5161 gtcggcaccg gtccgtacgc cgctgcctca ccaagggcgg tgttgcccga gtaggtcgaa
     5221 atcagcacga gtcccgccac tcgctccggc atccgcgccg ccgcggccag ggcgatcacg
     5281 ccgccttccg agaagccgcc gagccacacg cgctccacgt gggcggcgtc gagaacggca
     5341 gtcagctcct cgatgcgctc gtcgatgctg gctcggccgg gtgagggatc ggagaggccc
     5401 gtgccgagct tgtcgtactg aatgaagcgg aacgccgacg ccatgcgctg aatcgggcgc
     5461 cagaataccg gctgctccca catcatttcg atgtgctggg ccaacggtgg caccaacaag
     5521 aaggtcggct tcgcgtcgcc cacctgctga aacgcgacct tgccgcgcgc gctgctggcg
     5581 aaccgaatgc gcgggatgtc caccgctcga tggtgccaca atccaccgtc tttggaacga
     5641 ccgaactgtc gcgctcgggg cgctcctgga ttattcgaac ggaggcgctg atctcgctag
     5701 caacgagcgc ggatcggcag cacctctctc cctcattccg gcaaggaggc gtagcatgac
     5761 gccgggcgcg aacagccgtt tcgggggcgc ctggaagtgc aggacctcga gaagcgccgc
     5821 cgcgatttcc gggtcggcgt gagcgctggc gatcacccga ggtaggtaga agtttgcgat
     5881 gcgcatcttc agcgtcggac ggcccactgc ccccggatac agaaaaaggc gccccgccga
     5941 ggcactccag gcaacgtcga tgatgggagc cgcgctctcg agaaaggcgg tgccgagctg
     6001 gctcagccgc acgcgccttc ccaattgctg aagcaggagc gcttgcttgg ccgcaacggt
     6061 gatgccttgt ccgaacgtcg gattgaagct tgcgagcgcg tcagcgcata ccaaaagccc
     6121 tcgcgggaag tcgcgcatgc tgtcgtagtg gcggcgaatg ctcgacacga agcgcaaggt
     6181 gcgcgtctcg ctcagcggct cggagtcctc cagtacttcg gcgagcacgg gggtcgcgag
     6241 tgtacgcgcg aactgcgcaa agccggcggc gtcctgcggt ggccgctctc cccccatcga
     6301 ataaagagag acgatccagc gctctcggtc cagcgcgagc gcaacgccgc cgcgcggaac
     6361 cttcgggtcc gagacgacag cgatcgcaat cgcgccattc aggtggttcg gacgccgtgc
     6421 atacgtgcgg ctcgcgtagt gcgtgggaag cgtgatctct tcgatgcgtg gcgccgacag
     6481 acccaaggct aggagccagt ttggcagttc ggagtgccga cccgacgcat caatgacgag
     6541 atctgcctcg agcgtctctt cgtcgcccgg cacggacgct acgcgcagtc ccgtcactcg
     6601 cccggcttca tggagcaacc ccgtcactcg cgtccccgtc cggatgtgca cgttcgatag
     6661 gcgcgtcacg cggctctgga tcacgtgatc gagcaacggg cgagccaccg tcatcccgcg
     6721 cacgccgctg tcaccgagcg cgagccgccg acccgagaag atccacgccg cgtctcgcaa
     6781 gttgtcgcca gtcgggcatc cccgctcctc gagctctcgg cagagccccg gtaggagctg
     6841 ctcgagcgcc tggagaccac ctgccaagag gccatgtgca tgatgccctt gcgggacacc
     6901 cgcccgcggc aacggcgtgt cggggagctc tccgcgttcg accaccagga cttcatcgca
     6961 gacttcggcg agcacacggc ccaccaaaag cccggcgatg ctgccaccta caacgactgc
     7021 tctcgtcatg cggggagcgt ggcgactcgg cagcgcgctg tcgtgagcag aactactcag
     7081 ctcgcgcgag cgaccccgag ccgatgacga cctgagtagt tccactcacg acgcggaggc
     7141 gccaagtcgg cacgttattc gggtaacgcg cgccggggat gcccacgaag cgaacgccgc
     7201 gcgagggaga ggaaaagcca catgacaaca tcaattcagg tcgacacgga aacgaactcg
     7261 agccgcgcgg gcgcacaccc gggcgccgaa cgagcgcccg attctcgcgc caccgagcgc
     7321 tttgcccagc gggtgctcgg cgatctttca gcggcgatga cgggcgtgat ggtgcggctc
     7381 gggcacgaac tcgggttgta tcgggcgctc ggcgagcatg gcccactcac gtctggcgag
     7441 ctttcgctgc gcactttggt gctcgagcgc tacgcgcgcg aatggttgca ccagcaaaga
     7501 gcagccgggt acctcgacta cgacccacaa agcgcaaagt tcaggcttgc agaaggtgcc
     7561 tttgcggtcg tcgtggccga ggacagcccg gtgttcttgc tgcccgcgtt cgacatcgtt
     7621 gcctcgattt gggcggacga agcgcgcctc gagcaggcct ttacacgcgg cgagggcatc
     7681 ggctggggcg aacatgatcc acgcttgttt tcggggtgtg cgcgcttctt cggtgcggcc
     7741 tatgcgtcgc acctcgtgca agactggctg ccggtgctcg acggcgtcac cgacaagctc
     7801 gggcgcggcg caacggtagc ggacgtcggc tgcggacacg gcatctccac cgtgctgatg
     7861 gctcgagctt ttccggcatc gcgcttcgtc ggcttcgaca gccacgcggc atcgatcgac
     7921 gtcgcacggc agcgtgcgaa gcaggccggc gtctgcgagc gcgttcgctt cgagcgagcc
     7981 ctctccaccg acttcggtga cgagcgctac gaccttatct gcttcatgga ctcattccat
     8041 gacatggggg acgccgtggc cgcggcgcga cacgccgcga gccggctgac gaacggcggc
     8101 accgtgatgc tcgtcgaacc tctcgccgcc gagcgacccg aagaaaaccg cggaccaatc
     8161 gcggccatga actacgcggc atccacggcg ctttgcaccc cgaacgctct ctgccatcgg
     8221 cacggcatcg ctctcggcgc gcaagcggga cccgaggcgc tgcggcaggc gctcgcggag
     8281 ggtggactca ctcactttcg ggtagcggcc acgaccccgt tccatatggt gatcgaggct
     8341 cgatgatgat gcgtgtgcgc atgaggagcg tacgcggttg agggcatggt cggggccggt
     8401 atgcgagttc agcttgcgag tgcgctacgc ggcccctgcc gaggagactg gcggaggggc
     8461 gacctcggag gacgatggtg ggccgccctt cattgctgaa catttgcaca gttatcgccg
     8521 tcgagctacc gtagctcccg tccccatgct gaattgccta aaaatccgtg atttagcgat
     8581 cgtcgaggag ctcacgctcg acctgggccc cggcttgaat gtgctcaccg gcgagaccgg
     8641 cgcgggcaag agcgtgctgg tgggcgcgct cgagttggtg ttgggcggga aggcgaaggc
     8701 tgagctcgtg cggagcgggg cggcggcggg ggaggtggag gcgctgttcg aggtcggcgg
     8761 ggattcgccg gttcgagagc agctgcgcgc gctgaacttg ggagggtctg acgagctcgt
     8821 ggttcggcgt gtggtttcgt cttcggggcg agcgcgcgcg tacgtgaacg ggaagcttgc
     8881 gacgcaacag atgcttgcgg aactgacacg cgggctcgcc gatatttcgt cgcaacacga
     8941 gcatcacacc ttgaccgatc cgcgcacgca cctcggctat ctggacgcgt ttggcggcct
     9001 cgcagcgctc tgcgagcgg
//
LOCUS       DASTKS010000306         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1611477, whole genome shotgun sequence.
ACCESSION   DASTKS010000306 DASTKS010000000
VERSION     DASTKS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611477"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFQ35_06375"
     CDS             <1..856
                     /locus_tag="VFQ35_06375"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HET9930290.1"
                     /translation="PSSVVRNDELMVAIDVEEQSGRSLGADATVRLASAIRADWLLEL
                     YSERVGVEESLVWNTNAERVDALSRMTFGALVLDESRSAAAPSAEASRVLSEAVRTHG
                     ALEFLKSDELAEFRERLALVAKHYGESAKDEASVEAELLALCDGITSFEELRAQSSLA
                     TLQAKLPPKLRQLLERAVPESVRVGAGRSAKIHYEAGKPPWLESRLQDFFGSADGPRV
                     CDGKLAVTLHLLAPNGRAVQVTSDLAGFWERHYPSIRRELMRRYPRHSWPEDGRHAEP
                     PAPRPRRQ"
     gene            complement(913..>2691)
                     /gene="mutS"
                     /locus_tag="VFQ35_06380"
     CDS             complement(913..>2691)
                     /gene="mutS"
                     /locus_tag="VFQ35_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9930291.1"
                     /translation="HLELTASAAGKDATLLHAIDATRTPPGARLLRRRLLAPLLDVER
                     IRRRLDQVELFVMHSGLRGELAKVLSEVGDLERASMRASLNQATPRDLGALRDGLHAA
                     ARAVALLETLDDPESREVLGLNDAPDLVSEVAEALARALVDRPPVLAKEGEIFRAGYD
                     AELDELDELRRTGAERMVALEARLKQETQIATLKVRYTRVFGWYIEVSRGQASKVPAS
                     YRRKQTVATGERYTTPELDELADRILHAEERHRERELALLKSLIELAGSQGERIRALA
                     ARIARWDVAQGLAEVAHRHDYTRPVVDDSDVLLLRDARHPVVERSAAAGRFVPNDVEL
                     SVAGTHLWLLTGPNMAGKSTFLRQVALLTVLAQMGSYVPARSARIGCVDRVLSRVGAS
                     DNLSRGESTFMVEMRETAEILRSATRRSLVILDEIGRGTSTFDGLSIAWAVAEYLDQA
                     IACRALFATHYHELTAFADASRHADNYSVSAREHEGDVIFLHRVTRGAASRSYGVAVA
                     KLAGLPEPVLARARAILARLESERSAPLSAREKDQKRDQLDLFAPRQAELGAEKEVCD
                     TLRELDVDRLTGLQALELIARLKQRL"
BASE COUNT          436 a          916 c          902 g          437 t
ORIGIN      
        1 tccgagcagc gtcgtccgca atgacgaatt gatggtagcg attgacgtcg aggagcaatc
       61 gggacgctcg ctcggcgccg atgcgaccgt gcgcttggcg agcgcgattc gggcggattg
      121 gctcttggag ctctattcgg aacgggtcgg cgtcgaggag agcctggtct ggaacacgaa
      181 cgcggagcgt gtcgacgcgc tgagccgcat gactttcggg gcgctcgttc tcgacgagag
      241 ccgaagcgca gccgccccga gcgcggaggc gagccgagtc ttgagcgaag ccgtacgcac
      301 ccacggcgcg ctcgaattcc tgaagagcga cgagctcgcg gagttccgcg agcgcctggc
      361 cctcgtcgcg aagcattatg gagagagcgc caaagacgaa gccagcgtcg aggcggagct
      421 cctcgccttg tgtgacggga tcacgagttt cgaagagctt cgagcgcaga gctcgctcgc
      481 gactcttcag gcaaagctgc cccccaagct gcgacaattg ctcgagcgcg ccgtccccga
      541 gagcgtgcgc gtcggcgcag gtcgaagcgc caaaatccac tacgaggccg gcaagccacc
      601 gtggctcgag tcgcgtcttc aagacttctt cggcagcgcc gatggtcctc gcgtctgtga
      661 tggaaagctc gctgtcacgc tgcacctgct cgcgccgaac ggccgcgccg tccaggtcac
      721 gagcgatttg gcaggcttct gggagcgaca ctaccccagc atccggcgag agctgatgcg
      781 tcgctatcct cggcattcgt ggcccgagga tggccgacac gccgaacctc ctgcgccgcg
      841 gccgcgtcgg caatgagcct tttgcggcgc cccattccgg ccgtcctcag tgcctcaccc
      901 gtgcgcgcaa cttcacagcc gctgcttgag ccgggcgatt agctcgagcg cctgcagccc
      961 cgtgagccgg tcgacgtcga gctcgcgcag cgtgtcgcaa acctcttttt ccgcgccgag
     1021 ctcggcttgc cgaggcgcga acaaatcgag ttgatcgcgc ttctgatcct tctcccgtgc
     1081 cgagagaggc gcgctgcgct cgctctcgag ccgcgcgagg atggcgcgag ctcgcgcaag
     1141 aaccggttcg ggtaggcccg cgagcttggc gactgcaacg ccgtagctgc ggctggccgc
     1201 gccccgcgtc acgcgatgca agaagatcac gtcaccttcg tgttcgcgag cgctcacgct
     1261 gtagttgtcg gcgtgacggc tcgcgtccgc gaacgcggtg agctcgtgat agtgcgtcgc
     1321 gaagagtgct cggcacgcga tcgcctgatc gagatattcg gcgaccgccc acgcgatgct
     1381 gagcccatcg aacgtgctcg tgccgcgacc gatctcgtcc aagatgacga gcgagcgccg
     1441 cgtcgcgctg cgcaagatct cggccgtctc gcgcatttcg accatgaagg tgctctcgcc
     1501 gcgcgacagg ttgtcgctag ctccgacgcg tgagagcacg cggtcgacgc aaccgattcg
     1561 ggccgagcgc gccggcacgt agctgcccat ctgcgcgagc accgtgagca gcgcgacctg
     1621 ccgcaaaaac gtgctctttc cggccatatt gggcccggtc aggagccaga ggtgcgtacc
     1681 cgcgacgctg agctccacgt cgttcgggac gaagcgcccg gccgccgccg atctctccac
     1741 gacggggtga cgtgcgtcgc gaagcaagag cacgtcggag tcgtcgacca cggggcgcgt
     1801 gtagtcgtga cgatgcgcga cctcggcaag cccctgcgcg acgtcccagc gcgcgatgcg
     1861 cgccgcgagg gcgcggatgc gctccccttg agagccagcc aactcgatga ggctcttcag
     1921 gagagcgagc tcccgctcgc ggtgccgctc ctcggcgtgc aaaatgcgat cggcgagctc
     1981 gtcgagctcg ggcgtggtgt aacgctcccc ggtcgcgacc gtctgcttgc gacgatagct
     2041 cgcgggaacc ttgctcgctt ggcctcggct gacttcgatg taccagccga acacccgcgt
     2101 gtagcgcacc ttcaacgtcg cgatctgcgt ctcttgcttg agacgcgcct cgagcgcgac
     2161 cattcgctcg gcgccggtac gccggagctc gtcgagctcg tcgagctccg cgtcgtagcc
     2221 cgctcgaaag atctctccct ctttcgcgag cacgggagga cggtcgacca gcgcgcgagc
     2281 aagtgcctcg gccacctcgc tcacgaggtc gggcgcgtcg ttcaagccga gcacctcgcg
     2341 cgactctggg tcatcgagcg tctcgagcag cgccacggcg cgggcggcgg cgtgcaaacc
     2401 atcgcgcagc gctccgaggt cgcggggagt cgcttggttc agactcgctc gcatcgacgc
     2461 gcgctcgaga tcgccgactt cggacaggac cttcgcgagc tcaccacgca ggcccgagtg
     2521 catgacgaag agctccacct gatccagccg ccgccgaatg cgctcgacgt cgagcaacgg
     2581 cgcaagcagc cgccgccgca agaggcgggc gcccggtggc gtgcgggtag cgtcgatggc
     2641 gtgaagcagc gtggcatcct tccccgctgc gctcgccgtg agctcgaggt g
//
LOCUS       DASTKS010000307         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_718256, whole genome shotgun sequence.
ACCESSION   DASTKS010000307 DASTKS010000000
VERSION     DASTKS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718256"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VFQ35_06385"
     CDS             complement(<1..1057)
                     /locus_tag="VFQ35_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930292.1"
                     /translation="MGAFAKRKSSAVLPAALALSLQLLACGSSSSNAPAKSAAEAAPP
                     PAPPAPPASTGPEQQFAISDAPTAAENAPERPKMSGAAATAYASGMTAFQNGDLQGAK
                     AQFTQATQADSKAYQAFYSLGVVKERLGDTTGALSAYRQATLVVADYEPAIFAYSVLL
                     ARSGRSDEAVEYINGRLAAMPKSAAVSAAMAEIKSIQGDSGAAQRYAQEALKKNPDYR
                     PAMVTIARDHYRSRRLDLALYSLKGILDGYGPENPPRDKNNAEAHLIRGLIYKEQNLR
                     GPAMEELKKALDLRPDLVEARVHLATYLLEAGNPTEAAPLLEGALRYQIDHVIAHLNL
                     GDAYRLLGKVAESRRELE"
     gene            complement(1066..5016)
                     /locus_tag="VFQ35_06390"
     CDS             complement(1066..5016)
                     /locus_tag="VFQ35_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930293.1"
                     /translation="MPKPTAPTAQQLKAYKLLLEESHNYEAGAKDFRRTLTQIVRHHY
                     EERRRRVLSALDREITAEKHGLEEARNEAIARLERFIERYSGTNADPEASPDAMFRLA
                     ALYEERAREKQDTDIAVGLDPAIQLYSRIIREYPKYEEVAAVHYYLGHALTDAARLDE
                     GQQAWRALVCNNRYQVKDDPKKPGKIELPALPQDHDDKYWADWYNRHPLPLDQANAKA
                     GRVAFKGTKAGARGDTDEVTFADPYSGCQPIAQNTLPGEEPRYVAEIWWQIGNYHFDQ
                     VDPHGGPFSLNRAVSAYDHSMQYKKPPIYGVAMYKQAWTYFKQQRYHTAVDWFVKLLH
                     YTDEQEAKTGDPGADFRAEAYTYIAGSLTYVDFEGPPAESPYIPRSDVLDVEPDPIVA
                     ENKMAIGIKRVQDPALIPQDKKWTVEIYKSLAQEYIEITQNRNAVATLELTLQKFPMD
                     RDAPVMQNKTAELYDQLSRLAPEGSAARAEYSSKALEARTKLAQYVGTTPWVDANRDD
                     PEALATAEQLVRGGLKRAAADHTNFARGYYEKALELNDEGEQRALIEKAIAEYRLAET
                     GWAAYIEQEPTAIDSYESRFWLADARYWVVVLQVALGRSPSPGEVRSAREAAVAVRDS
                     NEDDKYLQPSAYYVVTIAEKVLEDEYRKFDQSKGQQGIEKRTEVHFTGDGGSRQVVRD
                     EVPQQVQDAIRARDEYNDRIPLDRDPQHNGLLYAYQSGDLYFVYGQFDSARKRFQPLY
                     DQYCGKNEWGYKAWEKLISMSNFEGNADESRRLAEGKSCAYNTETQKAEEAIRKPVQQ
                     GVAYLDARKLYEEAEKMPEGPARAKKWREAAAAYKVALDAAPDRDEAPEAAMNGAYAY
                     KQVGEYDKAIEMYELFISRYGSEQTLAKLKNGDPKANPPVAANPKKYEDRVGYLKGAY
                     DALAAAYVLFFNYPRAAETYDKISNNGNFKPADRREAARQALQLYASLGDRTGMTRAR
                     ERFQSLGASPKDMAEADFIVASADLKRWDPYSGDQGANATARRAAQRTMEDYYNANKN
                     KDAAAQYVVQAAYWSARTRKAVAAGDTNKWWEQTIAAFDKWKRLAPQTGGKSSALGSR
                     EAGMAAEGEFSLIDEELAKKFDYEAGLHRYRGTVVEVMQKYRADVVEAKKWDEKLQHV
                     IDAYVSAEWTAASLARQGTVWDSVRTGLYNTRPPELKMFDAKQEALLKKAESSDNPDL
                     QARADEIRVSVQQGWRDAREKELAGADELMVDRYGKSIMVARRYSVSNPAVTRAIRRL
                     AFFTDVVGEAKMKQYTSGVKDLNYTEGMFLRMRPGQVVSPQPVGSPPPLPVSIQ"
BASE COUNT          712 a         1670 c         1694 g         1054 t
ORIGIN      
        1 actcgagctc gcgccgcgac tcggcgactt taccgagcaa acgataggca tcgcccaaat
       61 tcagatgggc gatgacgtgg tcgatttgat agcgcagggc gccttcgagc aacggtgcgg
      121 cctcggtcgg gttgcccgcc tcgagcaggt acgtggcgag gtgaacgcgc gcctcgacga
      181 gatccgggcg caggtcgagc gccttcttca gctcttccat cgccggaccg cggaggtttt
      241 gctccttgta gatgagcccg cgaatcagat gcgcttcggc gttgttcttg tcgcgaggcg
      301 ggttctcggg gccgtagcca tcgaggatgc ccttcagcga gtagagagct agatcgagac
      361 ggcgcgagcg ataatgatcc cgcgcgatcg tgaccatcgc cggtcggtag tcggggttct
      421 tcttcagggc ttcttgggcg tagcgttgcg cggcgccgga atcgccttga atcgacttga
      481 tttcggccat ggccgccgag accgccgcgc tcttcggcat cgcggcgagg cgcccgttga
      541 tgtactcgac ggcttcgtcg gagcgcccgc tgcgcgcgag cagcaccgag tacgcgaaga
      601 tggccggctc gtagtcggcg acgacgagcg ttgcttgccg ataagcagac agggcgcccg
      661 tcgtgtcccc gagccgctcc ttcacgacgc cgagcgagta gaaggcttgg taggctttcg
      721 agtccgcctg cgtcgcctgc gtgaattgcg ccttcgcgcc ttgcaggtcg ccgttctgga
      781 acgccgtcat ccccgaggca tatgcagtcg ccgccgcgcc cgacattttg ggccgctcgg
      841 gagcgttctc ggccgcggtc ggggcgtcgc tgattgcgaa ctgttgctcg ggccccgtcg
      901 acgccggcgg agcgggcggc gctggtggcg gggccgcttc ggccgcgctc ttcgccggcg
      961 cgttgctcga ggaggagccg catgccaaaa gctggaggct gagcgcgagc gcggcgggca
     1021 gaaccgcgct cgacttccgc ttcgcgaatg cacccatcgt cgtcctcatt gaatgctcac
     1081 cggtagtggc gggggcgaac ccacgggctg cggagatacg acctggccgg gacgcatccg
     1141 caagaacatg ccctccgtgt agttcagatc cttaactccg ctcgtgtact gcttcatctt
     1201 tgcttcgccg acgacgtccg tgaagaacgc gaggcggcga atcgcccgcg tcaccgcggg
     1261 gttcgagacg ctgtaacggc gcgcgaccat gatggacttg ccgtagcgat cgaccatcag
     1321 ctcgtcggca ccggcgagct ccttttcgcg cgcgtcgcgc caaccctgct gaaccgagac
     1381 gcgaatctcg tctgccctcg cctgcaagtc ggggttgtcg ctgctctccg ccttcttcaa
     1441 gagcgcctct tgcttggcgt cgaacatctt cagctcgggc gggcgtgtgt tgtagaggcc
     1501 cgtgcgcacc gaatcccaaa ccgtgccctg acgcgccagc gaagccgcgg tccactcggc
     1561 ggagacgtac gcgtcgatga cgtgctgaag cttttcgtcc cacttcttcg cttcgaccac
     1621 gtcggcacgg tacttctgca tcacttcgac cacggtgccg cggtaacggt gcagccccgc
     1681 ctcgtagtcg aacttcttcg cgagctcttc gtcgatgaga gagaactctc cctcggcggc
     1741 cataccggct tcgcgcgaac cgagcgcgct gctcttgcca cccgtttgcg gggcgaggcg
     1801 cttccatttg tcgaacgcgg cgatggtctg ctcccaccat ttgttggtgt cgccggcggc
     1861 gaccgccttg cgggtgcgcg ctgaccaata agcggcctgc accacgtact gcgcggccgc
     1921 gtccttgttc ttgttcgcgt tgtaatagtc ctccatcgtg cgctgggcag cccggcgcgc
     1981 ggtggcgttg gcgccctgat cgccgctgta aggatcccag cgcttgaggt cggcgctcgc
     2041 tacgatgaag tcggcctcgg ccatgtcctt gggcgacgcg cccaggcttt ggaagcgctc
     2101 gcgcgcgcgg gtcataccgg tgcgatcgcc gaggctcgcg tagagctgca gagcctgccg
     2161 cgctgcctcg cgccgatccg ccggcttgaa gttcccgttg ttgctgatct tgtcgtaggt
     2221 ctcggcggcg cgcgggtaat tgaagaacaa gacgtaggcc gcggcgagcg cgtcgtacgc
     2281 ccccttcagg tatccgactc gatcttcgta cttcttcggg ttcgcggcga cgggcgggtt
     2341 cgccttcggg tcaccgttct tcagcttcgc gagcgtctgc tcgctgccgt agcgcgagat
     2401 gaagagctcg tacatctcga tcgccttgtc gtactcacct acttgcttgt aggcgtacgc
     2461 gccgttcatg gccgcttcgg gcgcctcgtc gcgatccgga gcggcgtcga gcgcgacctt
     2521 gtatgcggcc gccgcctcgc gccacttctt cgcgcgagcg ggcccttccg gcatcttctc
     2581 cgcttcttcg tagagcttgc gagcgtcgag gtacgcaacg ccttgctgca ccggctttcg
     2641 aatcgcttct tccgccttct gcgtctcggt gttgtacgcg cagctcttgc cttcggcgag
     2701 ccggcggctc tcgtcggcgt tgccctcgaa gttgctcatg ctgatgagct tttcccaggc
     2761 cttgtagccc cactcgttct tgccgcagta ctggtcgtag agcggctgga agcgcttgcg
     2821 ggcggagtcg aattgcccgt acacgaagta gaggtcgcct gactggtaag cgtacaggag
     2881 cccgttgtgc tgcgggtcgc gatcgagcgg gatgcgatcg ttgtactcgt cgcgagcgcg
     2941 gatcgcgtcc tgcacctgct ggggcacctc gtcgcgcaca acctgccggc tcccgccgtc
     3001 gccggtgaaa tgcacctcgg tgcgcttctc gataccttgc tgacccttgc tctggtcgaa
     3061 cttgcggtac tcgtcctcca gcaccttctc cgcgatggtg acgacgtagt acgcggaggg
     3121 ctgcaggtac ttgtcgtctt cgttcgagtc gcgcaccgcg accgcggctt cgcgcgcgga
     3181 acgcacctcg ccggggctcg ggctgcgacc gagcgcgact tgcagcacca ccacccagta
     3241 gcgggcgtcc gcgagccaga accgactctc gtacgagtcg atcgcggtcg gctcttgctc
     3301 gatgtacgcg gcccagccgg tctcggcgag gcggtactcg gcgatggcct tctcgatgag
     3361 ggcgcgctgc tcgccctcgt cgttcagctc gagcgccttc tcgtaatagc cgcgcgcaaa
     3421 attcgtgtga tcggccgccg cgcgcttcaa cccaccgcgt acgagctgct cggcggtcgc
     3481 gagcgcctcg gggtcgtcgc ggttcgcgtc gacccacggc gtcgttccca cgtactgagc
     3541 gagcttggtg cgcgcctcga gcgccttgga ggagtactcg gcgcgcgccg cggagccctc
     3601 gggagcgagg cggctcagtt gatcgtagag ctcggcggtc ttattctgca tgaccggagc
     3661 gtcgcgatcc atcggaaact tctggagcgt gagctcgagc gtcgcgaccg cgttccggtt
     3721 ctgcgtgatc tcgatgtatt cctgcgcgag cgacttgtag atctcgacgg tccacttctt
     3781 gtcttgcggg atcagcgcgg ggtcttgcac gcgcttgatg ccgatcgcca tcttgttctc
     3841 ggcgacgatc ggatccggct cgacgtcgag cacgtcgctg cggggaatgt agggactctc
     3901 ggcggggggg ccttcgaaat cgacgtaggt gagcgagccc gcgatgtacg tgtaggcctc
     3961 cgcgcggaag tctgcacccg ggtcacccgt cttcgcctct tgctcgtcgg tgtagtggag
     4021 gagcttgacg aaccagtcga ccgcggtgtg gtagcgctgc tgcttgaagt acgtccaagc
     4081 ctgcttgtac atcgccacgc cgtagatcgg cggcttcttg tactgcatcg agtggtcgta
     4141 cgcgctcacg gcgcggttca ggctgaacgg accgccgtga ggatcgacct ggtcgaagtg
     4201 gtaattgccg atctgccacc agatttccgc gacgtagcgc ggctcttcgc cgggtagcgt
     4261 attctgagcg atcggctggc agccgctgta cggatccgcg aacgtgacct cgtcggtgtc
     4321 accgcgcgcg cctgccttcg tccccttgaa tgcgacgcga ccggctttgg cgttcgcctg
     4381 atcgagcggc aacgggtgcc ggttgtacca atcggcccag tacttgtcgt cgtgatcctg
     4441 aggcagcgcg ggcagctcga tctttccagg cttcttcgga tcgtccttca cctggtagcg
     4501 gttgttgcag acgagcgcgc gccacgcctg ctgcccctcg tcgaggcgag ccgcgtcggt
     4561 gagggcgtgc cccaaatagt agtgcacggc cgcgacttcc tcgtacttcg ggtactcgcg
     4621 gatgatgcgt gaatagagct gaatcgccgg atcgaggccg accgcaatgt cggtgtcctg
     4681 cttctcgcga gcccgctcct cgtagagagc ggcgaggcgg aacatggcat cgggcgacgc
     4741 ctcaggatcc gcgttcgtgc cgctgtaacg ttcgatgaag cgctcgaggc gtgcaatcgc
     4801 ctcgttacgc gcctcttcga gcccgtgctt ctcggcggtg atctcgcggt cgagcgcgga
     4861 gaggacgcgt cgacgacgct cctcgtagtg gtgcctcacg atttgggtga gggtgcggcg
     4921 gaagtccttc gcgcccgcct cgtagttgtg cgactcctcg agcagcagct tgtacgcctt
     4981 gagctgttga gccgtcggcg cggtcggctt cggcaccgac ttgcgcaacg tgatgttggc
     5041 gccgctcggc ggcgcggctg cgttcaccgc cgacgtccct gcaaccgtcg acgcgccggt
     5101 agcgctcgag gccgctccac cgacgcccgt
//
LOCUS       DASTKS010000308         5046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_719074, whole genome shotgun sequence.
ACCESSION   DASTKS010000308 DASTKS010000000
VERSION     DASTKS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719074"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1608
                     /locus_tag="VFQ35_06395"
     CDS             238..1608
                     /locus_tag="VFQ35_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9930294.1"
                     /translation="MLTKTLVVDGITPVLAYQVLRRDAGLGSFLLESVVPGERWGRYS
                     ILGYRPQRETILRPEAGVDPFQKLGELLGPAQPGAADIATRFASAQVGLVPYDIVHYA
                     TKVPTWPEIEAEQRPIARLVQGATVIVFDNLTHTATIAAHEQGALERAERDLSLAKVL
                     PAFTPPDPAALPSDVDVSMSDADYGKVVERAKEFIRAGDAFQIVPARTFSTPTMGADP
                     FDVYRALRVLSPAPYMYLLELPANQGASPVAIAGASPETLVRVQGGRIALRPIAGTRR
                     RGRSIDEDEALAKEMLADPKELAEHVMLIDLARNDVGRVAKTGSVRVPHNMLVERYSH
                     VMHIVSEVDGELAAGLGPWDVVRATFPAGTLTGAPKVRAMQIIRELETRPRGAYGGAL
                     GYAMPNGDLDLAICIRTVVMQKDRFEVTAGAGIVADSVPELEAKETRNKARAALAAVH
                     AARQRR"
     gene            1704..2588
                     /locus_tag="VFQ35_06400"
     CDS             1704..2588
                     /locus_tag="VFQ35_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930295.1"
                     /translation="MLNHRLGLFGASAVLLFAVTHCGDSDRSSSGGLHSNGGKPSNMG
                     GAFQEGGASGAPLASGGTDDSGGTPGSANGGTGASGASHASGGGSSGGRADAGGSVAA
                     GGAAGGQPDGSGGGACAPAPAPGGVYDGDLYVGSAEELGAAHAYSRITGCLTLGVALG
                     DVSLPNLVSLGSLNAQSSTLTRLSLPNVQRIDGELWLYLNFSLTEVDLRRLKEVGGRL
                     YIHRNIMLSSLQLIALESVNGSTTRFDTESGITGNIRLPECMLDVPKARFESFVLTSG
                     LQNCSCSIECDLVTADGC"
     gene            2692..3201
                     /locus_tag="VFQ35_06405"
     CDS             2692..3201
                     /locus_tag="VFQ35_06405"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET9930296.1"
                     /translation="MPAWFEHLLPDSVRGLLQPLLSPPVLVGLGVFSIVTFIASIVGV
                     PYFLSHLPADYFTRRERLALGLPDTQNHGVHLLWKVLRNLLGAVLVLLGILMLVLPGQ
                     AILTILIGALLMDFPGKRRFERWVIARPSVMRALNRLRRRAGQPPIEPRSSWPPMSTR
                     SKPPSVTEK"
     gene            3331..4491
                     /locus_tag="VFQ35_06410"
     CDS             3331..4491
                     /locus_tag="VFQ35_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930297.1"
                     /translation="MARLGKQWMRDGRALLAAVLLHALSLTTLRHFAKPTPLIDARAT
                     PRPVSEEQQVDLDLAAPLVAARAENGGDEATAARSPASPRVAASIAARHPAAVAPVPD
                     DGASPNSASPNSEASLAPEESSEDGTALEPSQAIDLGVGPEGWRRWAVNPPVAPGSEA
                     AQGREPRFKAPSKSNTGGLLEGLEEHDRTVGLTPSGRVRTALFNAAHSDVAPQLGVAS
                     FRVTVRNDGSVEVALHGATDSEQKWREVAERAAADLRKNPPRIPPPRSGTRMLVEIKA
                     ESVLPNGAKVANLHGTRVEVDPPRIRTFEESKQAVKDKNPAAEALASPDYTMPLAVDV
                     PGVYLSHAGKVCNVRAGVTPFGLALSGGCDPSNIGAKPQRMVHTRIREESLF"
     gene            4574..>5046
                     /locus_tag="VFQ35_06415"
     CDS             4574..>5046
                     /locus_tag="VFQ35_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930298.1"
                     /translation="MQDENAFGLLFAFYDIEDVEYGLEPEEFVERFTEFRSTVLSSAA
                     EVVLGEAARVIDLGHALYFEVGDGDHGTDPIAWLRRVREALVARDFSVSAIVSHGGRW
                     LADDDADWPERDTLEGGYRLVHLSRPSEPLRRALAAEAATHGTDGTDGWGAGTYV"
BASE COUNT          865 a         1685 c         1655 g          841 t
ORIGIN      
        1 gccttcgcag cgagcctcga agacgcgccc ctcggtgtcg tgcaaagtga gcggcccgcg
       61 ccctttgcag cctaagcacc cgagtaaaag caaagccgag tagcgccacg accgaccgaa
      121 ggccatcgag ccgaacctat cgtcagagcc ctcagccggg caagtcgagc gcgcccgccg
      181 gccgcgatgc acaggccgcg aacgcacgcc gtacgttgcg ctataaaggt ccgtcccatg
      241 ctcacgaaaa cgctcgtagt ggacggcatc acgcccgttt tggcctatca agtgctgcgt
      301 cgagacgcag ggctcggctc gttcttgctc gaaagcgtgg ttcccggtga gcgctgggga
      361 cgctactcga ttcttggata tcgtccgcag cgtgagacga ttcttcggcc cgaagcgggt
      421 gtggatccgt ttcaaaagct cggcgagcta ctcggtccag cgcagccggg cgccgcggac
      481 atcgcgaccc gtttcgcttc ggctcaggtc gggctcgttc cttacgacat cgtccactac
      541 gcgacgaagg tcccgacgtg gccggaaatc gaggccgagc aaaggcccat cgcgcgcctc
      601 gtgcagggcg cgaccgtcat cgtcttcgac aacctgaccc acaccgcgac catcgccgcg
      661 cacgaacaag gcgctctcga gcgcgccgag cgcgacctat cgctcgccaa ggtgctgccg
      721 gcgttcaccc cgcccgatcc cgccgcattg ccgagcgacg tcgatgtgag catgagcgac
      781 gccgactacg gcaaggtcgt ggagcgcgcg aaggagttca tccgcgcggg cgacgctttt
      841 cagatcgttc cagcgcgcac cttttccacg cccaccatgg gcgcagatcc gttcgacgtg
      901 tatcgcgcgc tccgggtgct gtcgccggcg ccgtacatgt acttgctcga gcttcccgcg
      961 aaccaaggag cttcgccagt cgcaatcgcg ggcgcaagcc ccgagacgct cgtccgcgta
     1021 caagggggtc gtatcgcgct gcgacccatc gcgggcacgc gtcggcgagg ccgcagcatc
     1081 gacgaagacg aggcgctcgc gaaagagatg ctcgccgacc cgaaggagct cgcggagcac
     1141 gtcatgttga tcgatctggc gcgcaacgac gtgggtcgcg tcgcgaagac cgggtcggtg
     1201 cgagtcccgc acaacatgtt ggtagagcgc tatagccacg tgatgcacat cgtgagcgaa
     1261 gtggacggcg agctcgcggc gggcctcggg ccctgggacg tggtgcgcgc gaccttcccc
     1321 gccggtaccc tgaccggagc gcccaaggtg cgcgccatgc aaatcatccg agagctcgag
     1381 acgcgcccgc gcggcgccta cggcggcgca ctcggttacg cgatgccgaa cggcgatctc
     1441 gatctcgcga tttgcattcg gaccgtggtg atgcagaagg accgcttcga ggtaaccgcc
     1501 ggcgcgggga tcgtcgccga cagtgttccc gagctcgagg ccaaagagac gcgcaacaag
     1561 gcgcgcgccg cgctcgcagc cgtgcacgca gcgcgtcagc gtcgctgact gacccagaaa
     1621 tcggcgctcg gcggggggag cgcttgttcg cggcagcgct ttcgtgaatg ctactcggca
     1681 cacgacgacc ggggtgtcga ttcatgctga atcatcgatt ggggctcttc ggggcaagcg
     1741 cggtgttgct attcgcagtc acgcattgcg gcgactccga ccgctcctcc agcggcggac
     1801 ttcattcgaa cggtgggaag ccttccaaca tgggcggcgc gttccaagaa ggtggcgcta
     1861 gcggcgcgcc tctcgcaagc ggtggaactg acgacagcgg gggcacgccg gggagcgcta
     1921 atggcgggac tggcgcatct ggcgcttctc acgctagcgg cggcggttcg agcggcgggc
     1981 gcgctgacgc cggcggctcg gttgccgctg gcggcgccgc tggaggacaa cccgacggaa
     2041 gcggcggcgg ggcgtgtgct cctgctcctg cgccgggcgg cgtctacgac ggagatctgt
     2101 acgtgggctc ggctgaagag ctcggcgcgg cacacgctta ctctcgcatc accggctgcc
     2161 tgacgctcgg agtcgctctg ggcgacgtct cactgccgaa cctcgtgagc ctcggttcac
     2221 tgaatgcgca aagcagcacc ttgacgcgtc tctcgcttcc gaacgtgcag cgcatcgacg
     2281 gtgagctgtg gctttacctg aactttagcc tgaccgaagt cgacctgcga cgcctcaaag
     2341 aagtcggcgg gcgcctgtac attcaccgaa acataatgct ctcgagcctt cagctcatag
     2401 cgctcgagtc ggtcaacggc tcgacgactc gattcgatac ggagagcgga atcaccggca
     2461 acatccgact cccggagtgc atgctggatg tgcccaaagc gcgcttcgag tcgttcgttc
     2521 tcacgagtgg cttgcagaac tgttcgtgca gcatcgagtg cgacctcgtg accgccgacg
     2581 gctgctagga agctgctcag tccgtaatca cggccgtgcg tccggcgctc tggaacggcc
     2641 atttcccggg caatgcttgc atcgggaggc cgttttgccc atatcggatg ggtgcccgct
     2701 tggttcgaac acttgctgcc ggactcggtg cgcggcctgc ttcagccgtt gctctcgccg
     2761 ccggtgttgg tggggctcgg cgtcttcagc atcgtgacct tcatcgccag catcgttggc
     2821 gtgccgtact tcttgtcgca tctgccagcg gactacttca ctcgtcgcga acgcctcgcg
     2881 ctcggactcc cggatacgca aaaccacggc gtgcacctgc tctggaaggt gctgcgaaac
     2941 ctccttggag cagtcctggt cctgctcggg attttgatgt tggtgttacc cggtcaggcg
     3001 attttgacga tcttgatcgg ggcgttgctc atggactttc cggggaagcg ccgcttcgag
     3061 cgttgggtca ttgctcgacc gagcgtgatg cgggcgctga acaggcttcg acgacgggca
     3121 gggcagcccc caatcgaacc gcgttcgtca tggcccccca tgtcgacgcg ctcgaagccc
     3181 ccctcagtaa cggagaaatg agcccaacgg cggattctgc ccccaccgca gtcacccgca
     3241 cccggctgaa tgcgtctgcc acggcggcgc tgagtgaacc gaattcagta gcggtcgccc
     3301 cacgacgact acagcgctaa actcgccggc atggcgcggc tgggtaagca gtggatgcgg
     3361 gacgggcgtg cgctcctcgc ggctgtgctg ttgcacgcgc tttcgctcac cacgcttcga
     3421 cacttcgcga agccaactcc tctcatcgat gcgcgcgcca cgcctcgtcc cgtctccgag
     3481 gagcagcagg tcgatctcga tctcgctgcg ccgctcgttg cagctcgcgc ggagaatggc
     3541 ggggacgagg caaccgccgc gcgcagcccg gcgtcaccgc gtgttgccgc ctcgattgcg
     3601 gcacggcatc ccgccgccgt cgcgcccgtc ccggacgacg gtgcgtcgcc gaacagcgcg
     3661 tcgccgaaca gcgaagcctc cctcgctccc gaagaatcgt ccgaggatgg gacggcgctc
     3721 gagccatcgc aagcgatcga tctcggagtg gggccggagg gttggcgccg ctgggccgtg
     3781 aatcctcccg tagctccagg ttccgaagcg gcgcaaggac gcgagcctcg cttcaaagct
     3841 ccttccaaga gcaacacggg cgggctgctc gaggggctcg aagagcacga tcgcacggtg
     3901 gggctcactc cttcggggcg cgttcgcacg gcgctgttca acgccgcgca cagcgacgtc
     3961 gcgccgcagc tcggcgttgc gagctttcga gtgacggtgc gcaacgacgg ctcggtagaa
     4021 gtcgcgctgc acggcgcgac cgacagcgaa cagaagtggc gagaggtcgc cgagcgcgct
     4081 gccgccgacc ttcgcaagaa cccgccgcgc atcccgcccc ctcgatcggg cacgcgcatg
     4141 ttggtcgaaa tcaaggccga atcggtgctc ccgaacggcg ccaaggttgc gaatctgcac
     4201 ggtacgcgcg tcgaggtcga tccaccgcgc attcgcacct tcgaggaatc gaagcaggcc
     4261 gtgaaggaca agaacccagc cgccgaagcg ctggcgagcc cggactacac gatgccgctc
     4321 gccgtggacg tacccggcgt gtatctgagc cacgccggca aggtctgcaa tgtacgcgcg
     4381 ggcgtcacgc cgttcggcct cgccctgagc ggcggctgcg acccctcgaa catcggcgcc
     4441 aaaccgcagc gcatggttca cacacgcatc cgcgaagaga gcctcttcta gccagccact
     4501 tcgccgagcg cgacgacgcg ccgcttgaca cgagcgcgcg cgtgcggcct tttacccgct
     4561 caagtcgccc gccatgcaag acgagaacgc cttcggacta ctcttcgctt tctacgacat
     4621 cgaagacgtc gagtacggac tcgagccaga ggaattcgtc gagcgcttca ccgagtttcg
     4681 cagcacggtg ctttcgagcg cggccgaagt cgtgctcggc gaggcagcgc gagtgatcga
     4741 tctcggtcat gcgctctact tcgaggtcgg cgacggcgat cacggcacgg atccgatcgc
     4801 gtggctcaga cgggtgcgag aggcactagt cgcgcgcgac ttctcggtga gcgcaatcgt
     4861 gagccatggc ggtcgttggc ttgctgacga cgacgccgac tggcccgagc gcgacacgct
     4921 cgagggagga tatcgcttgg tgcatctcag ccgaccgagc gaacccttgc gccgagcgct
     4981 cgccgccgaa gcggcgactc acggcaccga cggaacggac ggctggggcg ccggcacgta
     5041 cgtgga
//
LOCUS       DASTKS010000309         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_51575,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000309 DASTKS010000000
VERSION     DASTKS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51575"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VFQ35_06420"
     CDS             <1..1391
                     /locus_tag="VFQ35_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930299.1"
                     /translation="MQGCPPGFAGFLSRVHPDDVRTLNEVAEKLSRGDEGQTFEHEFR
                     LLGNDGQWRWARSRGRIERRDGAVRMLGTLMDVTERRNLEEELRRAHRLESIGRLAGG
                     LAHDFNNLLAAMLGSLELLEEVCPPEGLDDLATARHGAERARDLTKQLLAFARKQPIV
                     LGAVDMAALVTKVERLLERLVGPTIELVISAERGLTVRADAAQLEQVLVNLVVNARDA
                     MPGGGLLNVRVRPDRHRLDVEREVVVLEVEDQGNGMDPETIRHIFDPFFTTKDTGTGL
                     GLASSYGIVQQHGGDILVDSELGRGAKFRVVLPRVATAGSVRVEPITKAEGAGCILVV
                     DDEESVRKTTARLLKSLGYDVVLASNCAEAVNIAETHPFAIDVLLCDIAMPERSGPEV
                     AREVTRVRPTIRTLFVSGYPEGADTNVASAGFLQKPYTRAALGAKLLALKAEPVTSGR
                     DSNQPTEVAQRK"
     gene            1639..2886
                     /locus_tag="VFQ35_06425"
     CDS             1639..2886
                     /locus_tag="VFQ35_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930300.1"
                     /translation="MPGSSSLEAVMSFSETAPPAQNRPPSLSSESTPRSQHGPSGSPG
                     HPSVELDSMILQDPFESSFAPDPFPPAAGPAASEPPPAGTVFERYHKLIAAAESARDH
                     ESAILAALDSAILGLPRSAPTSWSRAGSTASERAPHRPRLRSFNFERDVDWSEEPPKV
                     RDHGSEGDPEAKPKTSASTAPAPLASEVAVVFDGPLREPLRSKSADASELARKKRTFV
                     AVSVAAALVVGFTLGFVTGRGDERPANAETTEAANLVAAATAPTRAPEPAVPASLVES
                     APSAVATPTETVEGAPTAAVPPPALVQEPPARPAEPAALVGRRPAAPQATVGFDSRAA
                     LASLSKAGARAGVCVPPGEPGGSVVATVTFGRKGHVEDVSLSSATFRGSYSSDCIKGV
                     LASVRVRPFFGESIAVRKTLTIR"
BASE COUNT          550 a         1014 c         1026 g          488 t
ORIGIN      
        1 acatgcaggg ctgcccgccg ggtttcgctg gcttcttgag ccgcgtgcac ccggacgatg
       61 tccgcaccct gaacgaggtc gccgagaagc tcagccgcgg cgacgagggc cagactttcg
      121 agcacgaatt tcggctgctg gggaacgacg gtcaatggcg ctgggcgcga tctcgaggac
      181 gcatcgaacg acgcgacggc gcggtccgca tgttgggcac gctgatggac gtgaccgagc
      241 ggcgaaacct ggaggaagag ctgcgtcgag cgcaccgcct cgagagcatc ggacggctcg
      301 caggcggtct cgcccacgac ttcaacaatc tgctcgccgc gatgctcggg tcgctcgagt
      361 tgctcgaaga ggtttgcccc ccggagggac tagacgatct ggcgaccgct cgccacggtg
      421 ccgagcgcgc gcgcgatttg accaagcagc tgctcgcgtt cgctcgcaag cagcccatcg
      481 tgctcggagc cgtcgacatg gccgcgctcg tgaccaaggt cgagcgtcta ctggagcgcc
      541 tcgtggggcc gacgatcgag ctcgtgatca gcgccgaacg gggtctcacg gtgcgcgccg
      601 acgcggcgca gctcgagcag gtgctcgtca atttggtcgt caacgcgcgc gacgccatgc
      661 ccggcggcgg gctcctcaac gtgcgtgtac ggcccgatcg ccaccgtctc gacgtcgagc
      721 gcgaggtcgt ggtgctcgag gtggaagacc agggcaacgg catggacccg gagaccatcc
      781 gccacatttt cgatccgttc ttcacgacga aggacaccgg gaccgggctc ggtctggcga
      841 gcagctacgg catcgtgcag cagcacggcg gggacatttt ggtcgatagc gagctcggtc
      901 ggggcgcgaa gtttcgcgtc gttttgcctc gcgtcgcaac ggcgggtagc gtccgagtgg
      961 aaccgatcac gaaggctgaa ggcgcgggct gcattctcgt cgtcgacgac gaggaatcgg
     1021 taagaaagac cactgcgcga cttctgaaga gtctgggcta cgacgtcgtg ctggcgagca
     1081 actgcgccga ggcggtgaac atcgcggaga cccacccgtt cgcgatcgac gtgctcctct
     1141 gcgacattgc gatgcccgag cgcagcggcc ccgaggtagc gcgcgaggtg acccgcgtgc
     1201 gccccacgat tcgcactctg ttcgtttcgg gctacccgga gggcgccgac accaacgtcg
     1261 ccagcgcggg atttttgcag aagccgtata cccgcgcggc tctcggggcg aagctccttg
     1321 cgctcaaagc agagccagtc acaagcggcc gagactcgaa tcaaccaacc gaggtcgcgc
     1381 agaggaagta gctcgtgaaa ttcgagccat cgacgcactg gcaagccgag ccgctgcaaa
     1441 ccgtgcgcgt gagcacagaa ttcttcagtt acacccgagc tcgcacgggt gatgcgcagg
     1501 gagatcccag ggacagatcg gcccagagga ccttcgaggc cgtggtcgcc ggggatgcgc
     1561 tcggaaccgt cactcggata acgacttcga agcgcgctct tcaccttgcg tttgggcgaa
     1621 ggaccgagaa cattcgtagt gcctggttcg tcgagcctcg aggccgtcat gtcgttcagc
     1681 gagaccgcgc cgcccgccca gaaccgtcct cccagcctga gctccgagag cacgccacgc
     1741 tcgcagcacg gcccttcagg atcgcccggg catccatccg tcgaactcga ttcgatgatt
     1801 ctgcaagacc cgttcgagag cagcttcgct ccggaccctt ttccccccgc ggccgggccg
     1861 gccgcgagtg agccgccgcc cgcaggtacg gtgttcgagc gctaccacaa actcatcgcc
     1921 gccgccgaga gcgcgcgtga ccacgagagc gccattttgg cggccctcga ttccgccatc
     1981 ctcgggttac ctcggagcgc gccgacgtcg tggtctcgcg caggttcgac cgcaagcgag
     2041 cgagcgccgc atcgacctcg cctcaggagc ttcaatttcg agcgggacgt ggattggagc
     2101 gaggaaccgc cgaaggtgcg cgaccacgga tccgaaggcg accccgaggc aaagccgaaa
     2161 accagcgcga gcaccgcacc agcgccgctc gcgtccgaag tggcggtggt attcgacggc
     2221 ccgctgcgtg agcctctacg aagcaaaagc gcagatgcat cggagctagc gcggaagaag
     2281 cgaacgttcg ttgccgtctc ggttgcggca gctctcgtcg tggggttcac gctggggttc
     2341 gttacgggac gcggagacga gaggcctgcc aacgcggaaa cgacggaagc cgccaacctt
     2401 gtcgcggcgg cgaccgcacc tacccgcgcc ccggagcctg ccgttccggc gagcctggtc
     2461 gaaagcgctc cttccgcggt tgcgacgccg acagaaacgg tcgaaggcgc tcccactgcc
     2521 gcggtgcccc cacccgcatt ggttcaggag ccgcccgccc gccccgcaga gcccgccgct
     2581 ctcgtaggac gaaggccggc ggcgccccag gcaactgtcg gcttcgattc gagagcggcg
     2641 ctcgcctcgt tgagcaaagc gggcgcgcgc gccggtgtct gcgtaccacc cggtgaaccg
     2701 gggggctcgg tcgtcgcaac ggtcacgttc gggcgcaaag gacacgtcga ggacgtctcg
     2761 ctttcgagcg ccacgtttcg tgggagctac tcgagcgact gcatcaaggg cgtgctggcg
     2821 agtgttcgcg tgcggccatt cttcggcgag tccattgcag tcagaaagac tctgacgatc
     2881 cgctgaccgc ggttcgggag ctcgcggtta gaagaattgc ccattgacgg gcaacgtcac
     2941 ctgatgaatc gtccgaagtt cccgcgtgaa cccggcatcg aaagagggca gagccattgg
     3001 tttgccgccg tcgtcgcggc agctcccgtc gctcgccccc gacttgctcg aagcacttca
     3061 ggaaacgttc ggtaagca
//
LOCUS       DASTKS010000310         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_273444, whole genome shotgun sequence.
ACCESSION   DASTKS010000310 DASTKS010000000
VERSION     DASTKS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273444"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VFQ35_06430"
     CDS             <1..1341
                     /locus_tag="VFQ35_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930301.1"
                     /translation="LDVLLYLLENRQRVVGSAELLREVWRGVKIQPNTLTQTVATLRR
                     ALDDEDGSRIIETVRTRGYRFVASLSPRERTGTAEPSLCPAPHSMVGREREVAGLAAR
                     LESALQGTPNLVLITGAPGVGKTRLLTELGNMAKARGARLAVARCPETPRAPELWPWL
                     DLVRQLASASLLELIRASAAAAWLPELFPDVPRATAESNGAESFVLFQSLLALLREAA
                     SQDSVVLVLDDLNWADPASWLFLGFLARGLGNSKLLLALSYSEGQLTPTQARVVGATL
                     REDASRRMCLGSLRPEDVGSMLESEVGKTPPETMARLLEKTGGNPALLQQLLSASSPA
                     SSIRIDDTSALLSDETVRAAIEFQFAALSEPCLALLSTAAVLGRRFQLSRLCELTSVD
                     RALARDGLEEALRMKIVRRTGTPDELCFEHGLVRDVLLRRLSAAQRQELTTAQR"
     gene            1338..2699
                     /locus_tag="VFQ35_06435"
     CDS             1338..2699
                     /locus_tag="VFQ35_06435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930302.1"
                     /translation="MMTRPLAHGEPRRGQVLGGAELLSPVARGGMAVVWAARRAGTHE
                     SMPSLAVKVLLPGLATDVHFQKMLRAEARIAERIEHPNVCRVFGSGEARGIFYITMEF
                     LAGETLAALLQREQRIPCELAVQIAYNAARGLGAAHELVGADGRPLGVVHRDVSPQNI
                     MLTTRGEVKVVDFGVAKCFATDGSLTRSGYLKGKVAYMSPEQVYCEAVDARTDVFALG
                     VVLYQASTGRHPFAAGAQLGTLVNIAGPDPAPCPRTFVPGYPATLAAVLARALAKDPE
                     QRIQSMREFVNELERVGAELGATSGAVHRYLEAKSGAGAKAHAAPASRRPRGARRVDP
                     SPRGLLAGFEAARRSPLHTAAIGAAALLGALALEVRPVPGQRLPVPQRARTAVTSPEV
                     RLNAHRAKSLLAAMPQKSLFQATAADELNGGQAAATTIRKGKSAQTLSESETESFLLR
                     SRE"
     gene            2696..>3037
                     /locus_tag="VFQ35_06440"
     CDS             2696..>3037
                     /locus_tag="VFQ35_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930303.1"
                     /translation="MIPRHSYSAEHRRHLQLLPALVLLAVSSPRQALAETSNSAVAEA
                     LFRAGRAAMREHRLGDACDKFSESQRVEPKIGTLLNLALCHEQQGRLATAWAELRLAQ
                     AQAEALGKRAHL"
BASE COUNT          516 a         1022 c          962 g          537 t
ORIGIN      
        1 ctcgatgtcc tcttgtatct gctcgaaaac cgccagcgag tggtcggcag tgctgagctc
       61 ctgcgtgagg tgtggagggg agtaaagatt caaccaaaca cgctcactca gacggtcgcc
      121 acgttacgcc gagccctcga cgacgaggac ggttctcgaa tcatcgagac ggtgcgaacg
      181 cgtgggtatc gcttcgtcgc gtcactctcc ccgagggaaa ggacgggaac ggctgagccg
      241 tcgctctgcc ccgccccaca ctccatggtt gggcgagaaa gagaagtcgc gggtctcgcg
      301 gcgagactcg agagtgcgtt acagggaact cctaatctag ttttgattac gggagctcca
      361 ggcgtcggca agacgcgcct tctcacggag ctcggcaaca tggcgaaggc gcgcggcgca
      421 cgcttggccg tggcgcggtg cccagaaacc cctcgcgctc ccgagctctg gccctggctc
      481 gacctcgtgc gacagctcgc aagcgcttca ctcctcgagc tcatccgagc tagcgccgcc
      541 gccgcgtggc ttccggaatt gttccccgat gttccccgcg ccaccgcaga aagcaacggc
      601 gcggagagct tcgtcctgtt ccagtccctg ctcgcgttgc ttcgcgaagc cgcgagccaa
      661 gatagcgtgg tcctcgttct ggacgacctc aactgggcgg atcccgcatc atggctcttc
      721 ctcgggttct tggcgcgggg gctcggcaac tccaagctac ttctcgccct gagctattcg
      781 gagggccaac tcacgccgac ccaggcgcga gtcgtgggcg cgacactgcg cgaagacgcg
      841 agccgccgca tgtgtctcgg ctcattgcgt cccgaagacg tcggttcgat gctcgaatcc
      901 gaggtcggga agactccacc cgaaacgatg gcgcgactac tcgagaagac tggcggcaat
      961 ccagctctgc ttcagcaact cctgtcggca tcttcccccg cgagcagcat tcgaatcgac
     1021 gacacgtcgg cgctgctttc ggacgagacc gttcgcgcgg cgatcgagtt tcaattcgcg
     1081 gctctctcgg agccttgcct cgccctgcta tccaccgccg ccgttctggg tcgccgcttc
     1141 cagctctcgc gcctctgcga actcacgagc gtcgaccgcg ccctcgcccg cgacggcttg
     1201 gaagaggccc tacggatgaa gatcgtgcgg cggacgggca cgcccgacga actgtgcttc
     1261 gaacatgggc tcgtgcgcga cgtcttgctg cgtcggctct ctgccgctca gcgtcaagag
     1321 ctcaccacgg cgcaacgatg atgacccgac ctctcgctca cggtgagcct cgccgcggcc
     1381 aagtgctcgg cggcgcagag ctgctctcgc cggtcgctcg cggaggcatg gccgtggttt
     1441 gggcggcccg gcgcgcggga actcatgaga gcatgccttc gttggcggtg aaggtcctgc
     1501 tacccggctt ggcaacggat gttcacttcc agaaaatgtt gcgcgccgaa gctcgcatcg
     1561 ccgagcggat cgagcacccc aacgtttgcc gggtattcgg ctccggcgaa gcgcgcggca
     1621 tcttctacat caccatggag tttctcgccg gcgagaccct cgccgctctc ttgcaacggg
     1681 agcaacgtat tccctgcgaa ctggccgttc agattgccta caacgccgcg cgcggcctcg
     1741 gcgctgcaca cgaactcgta ggagccgacg ggagaccgct gggcgtggtt caccgcgacg
     1801 tctcgcctca gaacatcatg ctgacgactc gcggcgaggt gaaggtcgtc gacttcggcg
     1861 tcgccaagtg ttttgcgacg gacgggagct tgactcgcag cggttatctc aaggggaaag
     1921 tcgcctacat gtcccccgag caagtctact gcgaggccgt cgatgcccgc acggacgtgt
     1981 tcgctctcgg agtggtcttg taccaagcca gcaccggccg gcatcctttt gccgcgggag
     2041 cgcagctcgg cacgttggtg aacatcgcgg ggcccgatcc ggcgccctgt ccgcgcacgt
     2101 tcgttccggg ctatccggcg acgctcgctg cggtgcttgc tcgagcgctc gcgaaggatc
     2161 ccgagcagcg catccagagc atgcgcgagt tcgtgaacga gctcgagcga gtcggggcgg
     2221 agctcggtgc gacgagcggg gctgtccatc ggtatctcga agcgaagtcg ggggcaggcg
     2281 ccaaggcgca cgcggctccc gcgtcgaggc gtccacgagg agcgcgccgc gttgaccctt
     2341 ccccaagggg acttctggct ggtttcgaag cggcacgcag atctcctttg cataccgccg
     2401 ccatcggcgc cgccgcgctg ctcggagcgc tcgcgctcga agtacggcca gtcccggggc
     2461 agcgcttgcc agtccctcag cgggcgcgaa ccgccgtcac gtctcccgag gttcggctga
     2521 acgcgcaccg agcaaagtct ctgctggctg cgatgccgca aaagtcgctt ttccaagcga
     2581 cggccgctga cgagctcaat ggggggcaag ccgcggcaac gacgatccgc aaaggcaagt
     2641 cagcccaaac gctttcagag tcagagaccg aatcattcct gctccgcagt cgcgaatgat
     2701 acctcgccac agttactcgg ccgaacaccg gcgacacttg cagctcttgc ctgcactcgt
     2761 cttgctcgcg gtgagctccc ctcgccaggc tctcgcggag acctcgaact ctgcagtggc
     2821 cgaggcactg tttcgagctg gccgtgccgc gatgcgtgag catcgcctgg gggacgcctg
     2881 cgacaaattc agcgagagtc agcgcgtcga gccgaagatc ggcaccttgc tgaacctcgc
     2941 actttgccac gagcaacagg ggcgtctcgc caccgcttgg gcagagctcc gtctggcaca
     3001 agcgcaagcc gaggcgctcg gcaagagggc acatctc
//
LOCUS       DASTKS010000311         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_943447, whole genome shotgun sequence.
ACCESSION   DASTKS010000311 DASTKS010000000
VERSION     DASTKS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943447"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFQ35_06445"
     CDS             complement(<1..367)
                     /locus_tag="VFQ35_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930304.1"
                     /translation="MSSKDRNPTPCAIRLVDVLRQLPERELESLIERLKIKVDEAKRI
                     DVPSQVARSLIQLPELRDPNVLPGPTRELLHRIAEARGVLVTPSLPAAVEPLIARGVV
                     FARNGTRGIELLLPIAHMLV"
     gene            709..1995
                     /locus_tag="VFQ35_06450"
     CDS             709..1995
                     /locus_tag="VFQ35_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9930305.1"
                     /translation="MILRDPVHGLVSFESAEESIVPALLEASEVQRLRRVRQMGLASL
                     AYPGADHTRFSHALGAAHVMTRLIRRLRVIHDELPFWQRLTSERARDALAAALLHDLG
                     HGPFSHLFEEVMPEGRRHEEWTARMIMNPEGDVHRILSRYDAGLPARVVDLVRGKHEL
                     TYLASAVSGTFDVDRCDYLLRDAHFTGVGYGAYDLDWLLRSLRFGLSSGDGGPALAID
                     GAKGMPAIESFLLARLFMFQQVYFHKTERASEWMISRILRRVLELIADGVRVPGTPRA
                     IVSIGETGDAELDDYAELDDATLWVALSGYRDAKDPLLSDLSRRFYSRKLFKTIELFG
                     EQAEPEARTIALERAREVARARGLDPEVYVGLDAASTVAFDEKRDPLMVVFPDGEARL
                     LSNVSFLLGRLVNQRLSRVRLIFAEELRDDIRQALA"
     gene            1992..2639
                     /locus_tag="VFQ35_06455"
     CDS             1992..2639
                     /locus_tag="VFQ35_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930306.1"
                     /translation="MTKSVFALLALLLTSSVAHAEVEAESDGWDPGERVASRVKGEPR
                     FSDRMRSGDGVYDRFDGDLFLSLGAGVEWSHGVRPAIEARALYYYSVGLVIGYSDAFD
                     RDAFVRRTGWAGLDLRPLFLPRWALDKEFQLSFWDLTLDSVSLGAAAFLAEPRRGDTK
                     FSVEFSAGFGLPLFARARGLWLEGRGFWRPSYEDASTGLLVSLAYYAPVLTPFVK"
     gene            complement(2620..>2813)
                     /locus_tag="VFQ35_06460"
     CDS             complement(2620..>2813)
                     /locus_tag="VFQ35_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HET9930307.1"
                     /translation="KLLRGFEPTETTSVHHLADPRFDRAVRDFLERETSALREQIANA
                     KDEAGLKALSALDSTSRTE"
BASE COUNT          429 a          933 c          925 g          526 t
ORIGIN      
        1 tcacgagcat gtgtgcgatg ggcagcagca gctcgatccc gcgggtgccg ttccgagcga
       61 acacaacgcc gcgcgcaatg agaggctcca cggccgccgg caagctcggc gtgaccagca
      121 ctccccgcgc ctcggcgatg cggtgcaaca gctcgcgcgt cggacccggc agtacgttgg
      181 ggtcgcggag ctccggcagc tggatcaaag accgggcgac ctgcgacgga acgtcgattc
      241 gcttggcctc gtcgaccttt attttcagcc gttcgatcag cgactcgagc tcgcgctcgg
      301 ggagctggcg cagcacgtcg accaagcgga tggcacaagg agtcgggttg cggtctttgg
      361 aggacatggt gggataccca aggagccgaa ccaagcagct ccgctcgggc gctggcgggg
      421 tccccctccg ttggacccat gcgagcgcgc cgtttcgttt tcagccggga gagtaaccag
      481 actactcaag ctcgaaaacg ccccgtccgc aagtgcgaga gacgcctctt tttcacggat
      541 ttcgccgaag cgcgcgcatt tttggcttgg gacgaccaga attcttgggc tcgagccccc
      601 agaaccggac tttttcagtc gttttggtga cggctttcgc cacaatcgac gagcctcgtc
      661 gtcgatccct cggcgagcgg cctcgaagcg gatataccct gccgccgaat gattcttcgg
      721 gacccggttc acggcctcgt gtcgttcgag tccgccgagg agtcgatcgt gcctgcgctc
      781 ctcgaagcga gcgaggtgca acggctccgt cgcgtccgcc aaatgggact cgcgtcgctc
      841 gcctaccctg gcgccgacca cacgcggttt tctcacgcgc tcggcgcggc ccacgtaatg
      901 actcgcctca tccggcggct ccgcgtgatc cacgacgagc tcccgttttg gcagcggctg
      961 acgagcgagc gcgcgcgcga cgccctcgcc gcggcgctgc tccacgacct cggacacggc
     1021 cctttctcgc acctcttcga ggaagtgatg cccgaaggcc gccgccacga agagtggacg
     1081 gcgcggatga tcatgaaccc ggagggagac gtgcatcgga tcctttcgcg ttacgacgcc
     1141 gggctccccg cgcgcgtcgt agacctcgtg cgcggcaaac acgagctcac gtacttggcg
     1201 agcgcggtga gcgggacgtt cgacgtagat cgctgcgact acttgttgcg cgacgcgcac
     1261 tttaccgggg tcggttacgg cgcctacgac ctcgactggc tccttcgcag cttacgcttc
     1321 ggcctatcga gcggggacgg gggacccgcg ctcgccatcg acggagcgaa gggcatgccc
     1381 gcgatcgagt cgttcttgct agcgcggctc ttcatgtttc agcaggtgta ttttcacaag
     1441 accgagcgcg ccagcgagtg gatgatctca cgcatcctgc gccgcgtgct cgagctcatt
     1501 gcggacggtg tgcgcgtccc cggcacgccg cgcgcgatcg tctcgatcgg cgagaccggt
     1561 gacgccgagc tcgacgacta cgcggagctc gacgatgcga cgttgtgggt cgcgttgtcc
     1621 ggctaccgcg acgcgaaaga cccgctcctc tcggaccttt ctcgccggtt ttacagccgc
     1681 aaactcttca agacgatcga gctcttcggc gagcaggcag agcccgaagc gaggaccatc
     1741 gccctcgagc gcgcacgaga agtggcgcgg gcgcgcggac tcgacccaga ggtgtacgtc
     1801 gggctcgacg cggcttccac cgtcgcgttc gacgagaagc gcgatccgct gatggtggtg
     1861 ttccccgacg gcgaggcgcg gctcttgtcg aacgtctcct tcttgctggg acgcctcgtg
     1921 aaccagcgac tctcacgcgt acgcttgatc ttcgccgaag agctgcgcga cgacatccgg
     1981 caggcgttgg catgacgaag agcgttttcg ccttgctcgc gctgctcctc acgagctcgg
     2041 tcgcccatgc ggaggtcgag gccgagagcg acggctggga tcccggcgag cgggtcgcct
     2101 cgcgcgtgaa gggcgagccg cgcttttcgg atcgcatgcg gagcggcgac ggagtctatg
     2161 atcgcttcga cggcgacttg ttcttgtcgc tcggcgcggg ggtcgaatgg agccacggcg
     2221 tgcgtccggc gatcgaagcg cgcgccctct actactacag cgtcgggctc gtcatcgggt
     2281 actccgatgc tttcgatcgc gacgctttcg tgcggcgaac gggctgggcg gggctcgact
     2341 tgcgcccgtt atttctcccc cgctgggcgc tcgacaagga gtttcagctc tcgttttggg
     2401 acctgacgct cgattcggta tcgctcgggg cagccgcttt tctcgccgag ccgcgccgcg
     2461 gtgacaccaa gttctcggtg gagttctcgg ccggcttcgg ccttccactc ttcgcgcggg
     2521 cacgcggcct ctggctcgag gggcgcggct tctggcggcc gtcgtacgaa gacgcctcca
     2581 cggggctcct cgtatcgctc gcctattacg cccccgtgct cactccgttc gtgaagtaga
     2641 gtcgagcgcc gagagcgctt tgaggcccgc ctcgtccttc gcgttcgcga tttgctcccg
     2701 cagcgcggaa gtctctcgct ccaaaaagtc gcgaaccgct cggtcgaagc gcgggtcggc
     2761 caagtggtga acgctggtcg tttcggtcgg ctcgaagccg cgcagcagct tgt
//
LOCUS       DASTKS010000312         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_51986,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000312 DASTKS010000000
VERSION     DASTKS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51986"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VFQ35_06465"
     CDS             <1..1989
                     /locus_tag="VFQ35_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9930308.1"
                     /translation="QAVVVSDAEAGLALLAELAQHERGRADVIAACPAYVAGAPLLSA
                     DARVLGRVADQVSYAPNDEALVRSLFGNALLVNTPSEAAEVARELGTIAVALDGTVAR
                     PDGIVSGGSGDDVASGMIEQKREMTELTEELRRLEADSTRLHADHNALRARVSEVSTA
                     LERARQEAHQGELAHVTAEKDLARTLAEIARVEKRLAVISNELGELGRTVQEAEAVES
                     ESRAQLDTVRGELEHLQHSLARAEDNAAAWRERVAAQASLVTERRVRLAQVKEQLGAA
                     RAGRERVEAGLAEVEARAQRLTVELEEAAASYGETAARILIAREARVGATERARLAHE
                     ALDAARERLEAVRAALNAQEGELRKQRDELAAFDEAARKHEMAVQRIELERDHLVNGI
                     RERFRGLELARVVGDYHARPAPDAEHRRRIDELTQLIDRMGPVNLDAKAEHEDAERRF
                     KELNDQKVDIEKALEELDNAIRHMNKESRKRFRETFEEVNNLFKKTFSKMFRGGRAEL
                     QLTNPDDMLGSGVEIIAQPPGKKIGTIELMSGGEKALTAVSLIFAIFQHRPSPFCILD
                     EVDAPLDEANVARYNEMVRAMTSHSQFILITHIKKTMQSVDVLYGVTMGEPGVSRIVS
                     VKVNEHAVVRSDSIVARSGPREASTTPPAADEAVAGVA"
     gene            2015..2179
                     /locus_tag="VFQ35_06470"
     CDS             2015..2179
                     /locus_tag="VFQ35_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930309.1"
                     /translation="MALDLVVTVRGKVQGGESARLSLEDNGYYWFLHPFFERMRAQSG
                     KSARRVRESA"
     gene            complement(2202..>3015)
                     /locus_tag="VFQ35_06475"
     CDS             complement(2202..>3015)
                     /locus_tag="VFQ35_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930310.1"
                     /translation="GVGGRTGAGSNKGAGGAGKASGSGGKGEATAGSGVGGKLSTGGG
                     GRAPIAGASSGGNSPGGGNSPGGGNSPGGSNSNTGEGGTLVAGMGGTTDPTGGQGSGG
                     TPDEPTPFFSEYVEGSGSYKALEIAANGPRDLSGCQIEIFTNGATKRWRSIELSGSVS
                     REQVAVICSSELAALVPACLPSGGLSFTGNDVLLLSCSGNIVDAFGQVGNDPMGVGWG
                     APNRRTSDVTLRRFCDVTHGDAEPNDVFDPALEWSEAPIDSFEDLGAFCSSP"
BASE COUNT          547 a         1080 c          946 g          442 t
ORIGIN      
        1 caagccgtcg tcgtctcgga cgccgaggcg ggcctcgcgt tattggccga gctcgcccag
       61 cacgagcgcg gccgtgccga cgtcatcgcg gcttgcccgg cgtacgtcgc gggcgcgccg
      121 ttgctctccg ccgacgcccg cgtgctcggt cgcgtcgccg accaggtgag ctacgcgccg
      181 aacgacgaag cgctcgtgcg ctcgctgttc ggcaacgcgc tactggtgaa cacgccgagc
      241 gaagccgccg aagtcgctcg cgagctcggg acgatcgcgg ttgcgctcga cggcaccgtc
      301 gcgcgtcccg acggaatcgt cagcggtggc agcggcgacg acgtcgcaag cggcatgatc
      361 gagcagaagc gcgagatgac cgagctcacc gaggagctcc ggcggctcga agccgactcg
      421 acgcgcctgc acgccgacca caacgccctt cgagcgcgcg tgagcgaagt ctccaccgcc
      481 ctcgagcgcg ctcgacaaga ggcgcatcag ggagagctcg cgcacgtcac cgccgagaaa
      541 gacttggccc gaacgctcgc cgaaatcgcg cgcgtcgaga agcgactcgc cgtgatcagc
      601 aacgagctcg gggagctcgg ccgcaccgtg caggaagccg aggcggtcga aagcgaatcc
      661 cgcgcccagc tcgacaccgt tcgcggcgag ctcgagcacc tgcagcactc cctcgcgcgc
      721 gccgaagaca acgccgcggc ctggcgcgaa cgagtggcgg ctcaggcttc cctcgtgacc
      781 gagcgccgag tccggctcgc ccaggtaaag gagcagctcg gtgcggctcg cgccggacgc
      841 gaacgtgtcg aggcaggact cgccgaggtc gaagcgcgcg cgcaacgctt gaccgtcgag
      901 ctcgaagagg ccgccgcatc ctacggcgag accgcggcgc gcatcctcat cgcgcgtgaa
      961 gcgcgcgttg gcgcgaccga gcgcgcgcgc ctcgctcacg aagctctcga cgctgcccgc
     1021 gagcgcctcg aagcggtccg tgccgcgctc aacgcgcagg aaggcgagct ccgtaaacaa
     1081 cgcgacgagc tcgccgcctt cgacgaagcc gcccgcaagc acgaaatggc ggtgcagcgc
     1141 atcgagctcg agcgcgatca cctcgtaaac ggcattcgcg agcgcttccg cgggctcgag
     1201 ctcgcgcgcg tcgtcggcga ctaccacgcg cggccggctc ccgacgccga gcatcggcga
     1261 cgcatcgatg agctcacgca gctcatcgac cgcatgggcc cggtgaacct cgacgcgaag
     1321 gcggagcacg aagacgccga acgccgcttc aaggagctga acgaccagaa ggtcgacatc
     1381 gaaaaggccc tcgaagagct cgacaacgcg atccgtcaca tgaacaagga gtcgcgcaag
     1441 cgcttccgcg agacgttcga ggaggtcaac aacctcttca agaagacctt ctcgaagatg
     1501 ttccgcggcg gtcgcgccga gctccagctc acgaaccccg acgacatgct cggcagcggc
     1561 gtcgaaatca tcgcgcagcc cccaggcaag aagatcggca ccatcgagct catgagcggt
     1621 ggagagaaag ccctcaccgc cgtctcgctc atcttcgcca tcttccagca tcgcccctcg
     1681 cccttctgca tcctggacga agtcgacgca ccgctcgacg aagccaacgt ggctcgttac
     1741 aacgagatgg tgcgcgccat gacctcgcac tcgcagttca tcctcatcac gcacatcaag
     1801 aagacgatgc agagcgtcga cgtcttgtac ggtgtgacca tgggcgagcc cggcgtgtcg
     1861 cgcatcgtca gcgtcaaggt caacgagcac gcagtcgtgc gcagcgattc gatcgttgct
     1921 cgcagcggcc cacgcgaggc ctccaccacg ccgcctgccg ccgacgaagc ggtggccggc
     1981 gtcgcctgag ccgcggcttc ggacctacag gcgcatggcg ctcgacctcg tcgtgacggt
     2041 tcgcggcaag gtccagggcg gcgaatcggc tcggctctcc ctcgaagaca atgggtacta
     2101 ttggttcttg cacccattct tcgaacgcat gcgggcgcag tctggtaagt ccgcgagacg
     2161 agtccgcgag tccgcgtgac tagtcggggc gcattgcgcg actacgggga cgagcagaac
     2221 gcgccgagat cttcgaagga atcgatcggc gcttccgacc attcgagcgc agggtcgaat
     2281 acatcgttcg gctccgcatc gccgtgagtg acgtcgcaga agcgtctcaa ggtgacgtca
     2341 ctcgtccgtc gattcggcgc tccccagccc acgcccatcg ggtcgttccc cacctgccca
     2401 aacgcatcga cgatatttcc cgaacacgac aggagcagca cgtcattgcc cgtgaacgac
     2461 agccctccgc tcggcaagca cgccggaacc aaagcggcga gctctgacga gcaaatcacg
     2521 gcgacttgct cgcggctcac cgaccccgaa agctcgatcg agcgccatcg cttcgtggcg
     2581 ccattggtga agatctcgat ttggcatccc gaaagatcgc gcggaccgtt cgcggctatt
     2641 tcgagcgcct tgtagctgcc gctcccctcc acgtactcgc tgaaaaatgg cgtcggctca
     2701 tccggtgttc cgccggagcc ctgtcctccc gtcgggtcgg tcgtgccgcc catgcctgca
     2761 acgagggttc cgccttcacc ggtgttcgag ttgctgccgc caggcgagtt gccgccacca
     2821 ggtgagttgc cgccaccagg tgagttgccg ccagagcttg cgcccgcaat gggagccctg
     2881 cctccgccgc cagtgctcaa cttgccgccg acaccgcttc cggccgtcgc ttcccctttt
     2941 ccgccggagc cagacgcctt gcccgcaccg cccgcaccct tgttggaacc ggcacctgtt
     3001 cgcccgccga caccg
//
LOCUS       DASTKS010000313         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_943494, whole genome shotgun sequence.
ACCESSION   DASTKS010000313 DASTKS010000000
VERSION     DASTKS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943494"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFQ35_06480"
     CDS             <1..1583
                     /locus_tag="VFQ35_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9930311.1"
                     /translation="CASSEGVDALLDDMPAPFKLSPAERLGRARAFADAGRVEELERE
                     LGLCGAEAQKPAGVREHLQGLARLRARRELPRAAELLCRAAAENAGSAAERKFLAARA
                     HERNGDDAAARALYEQVLRGYPNSSFAEQAEYRSAQLAYSSGKFSVALEAYDRYLRRH
                     GARGAHSDDVRDERAISLLALGRPTASKELRALGDAAKDERTRLRYLELEGVALQREK
                     KLELARARFNEVMQRAPLSFFALAAEARLQALGAPLPTPSPSRAKGALAPLEPKLPAA
                     AELLHRVGLDREAEAALGEAESGVLRDYAGRGEQALCQLYGELAPAERRYRWGQRAAT
                     SEELSSLPSDERRWLWDCVYPRPYSSLVTAFGDENGVEPEFIYAVMRQESSFRPEVAS
                     GAQAQGLLQLMPSTASRLAQELNLSGALDLRQPPQNVRLGAYYLRKLQGWFANNPALV
                     AAGYNAGPVAVLRWLRGSPDLDLDVFVARIPYDETRTYVERVLSNYARYRYLAAQTAP
                     IPFALRLPAPNVENAELF"
     gene            1698..>2285
                     /locus_tag="VFQ35_06485"
     CDS             1698..>2285
                     /locus_tag="VFQ35_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930312.1"
                     /translation="MESRDPGISSIGPHAASASQPEVAQGGAPPVTQLTLFLGILCSL
                     LLTAVVLFLGFSWSSYRETYAAVDTGFRVGGSRLVEITLIREDLRNLSCASDVRVGAL
                     RCGFDGAHAPVSGLPERETLRPYNTVKNELLLVAGLWSELAEPEALPRARFTVVCDYH
                     VTSVIKSAELRWAPSGKFDRLDRSVPVGFVKNCQIP"
BASE COUNT          347 a          744 c          793 g          401 t
ORIGIN      
        1 tgtgcgcgag cagcgaaggc gtcgacgctc tcctcgacga catgcccgcg ccgttcaagc
       61 tgagcccggc tgagcgcctc ggccgcgcgc gcgccttcgc cgacgcgggc cgcgtcgaag
      121 agctggagcg cgagctcggt ctttgcggcg cggaggcgca gaagcctgcg ggcgtgcgtg
      181 agcacctgca aggtttggcg cggcttcgcg ctcgccgcga gttgcctcgc gcggctgagc
      241 tcttgtgtcg cgctgcggcc gagaacgcgg gcagcgcagc ggagcgaaaa ttcttggccg
      301 cgcgcgcgca cgagcgaaac ggggacgacg ccgcggcgcg tgccctgtac gagcaggtgc
      361 tgcgaggcta cccgaatagc tcgttcgccg agcaagccga gtaccggagc gcgcagctcg
      421 cgtattcttc cggcaagttc agcgtcgcac tcgaggccta tgatcgctat ctgcggcggc
      481 acggcgcgcg cggagcacac agcgacgacg tgcgcgacga acgcgccatc tctctgctcg
      541 cgctcgggcg ccccaccgcg agcaaggagc tgcgcgctct cggtgacgcg gcaaaggacg
      601 agcgcactcg gctgcgttat ctcgagctcg aaggggtcgc tctgcaacgc gagaagaagc
      661 tcgagcttgc tcgcgctcgc ttcaacgaag tcatgcagcg agcgccgctc tctttcttcg
      721 cgctcgccgc cgaggcacgg ctgcaagcgc tgggagctcc tctgcccacg ccgtcgccga
      781 gccgcgcgaa gggagcgctc gcgcccctcg agccgaagct cccggcggct gcggagcttc
      841 tgcatcgcgt tggtctcgat cgagaggccg aagcggcgct cggcgaagcg gaaagcggag
      901 tgctgcggga ctacgcaggt cgcggcgagc aagcgctgtg tcagctttat ggggagctcg
      961 cgcccgcgga gcgtcgctac cgttggggtc agcgcgccgc gacgtcagaa gagctgagct
     1021 cgctgccttc ggacgaacga cgctggcttt gggactgcgt ttaccctcgt ccgtactcct
     1081 cgctcgttac cgctttcggc gacgagaacg gggtcgagcc agagttcatc tacgccgtca
     1141 tgcgccaaga gagctcgttt cgccccgagg tcgcttcggg cgcgcaggcc caaggtctct
     1201 tgcagctcat gccgagcacc gcatcgcgtc tcgcgcagga gctcaacctc tcgggggcgc
     1261 tcgatttgcg gcagcctccg caaaatgttc ggctcggggc ctactatctt cgcaagttgc
     1321 agggctggtt cgcgaacaac cccgccttgg ttgccgcagg ttacaacgcg gggccggtcg
     1381 ccgtgctgcg ctggttgcgc gggtcgccgg acctcgacct ggacgtgttc gtcgcgcgaa
     1441 ttccgtatga cgagacgcgc acttacgtcg agcgagtgct ctccaattac gcccgctatc
     1501 ggtacctcgc ggctcaaacg gcgcccatcc cgttcgcctt gaggctcccc gctcccaacg
     1561 tcgagaacgc cgagctattc tgagttgacc tagcgcggct gatggaggga gagagcctgt
     1621 gagccgagtt cgcgcgctcg tgccagcctg gcacggggct tgcgactctc cgcttgcgta
     1681 tggagcgcac gggagcgatg gagtcgagag atccggggat ttcgagtatc gggccgcacg
     1741 cggcgagcgc gtcgcaaccc gaggtggcac aaggtggcgc accgccggtg acacagctga
     1801 ccctgttctt gggaatcctg tgttcgctgc tgctgacggc ggtggtgctg ttcctcggct
     1861 tttcgtggtc gagttaccgt gagacctacg cggccgtaga tacgggtttc cgcgtggggg
     1921 gatcccggct ggtggagatc acgttgatcc gcgaggactt gcggaacctc tcctgcgcct
     1981 cggacgtgcg cgtcggcgcg cttcgctgcg gtttcgacgg tgctcacgcg ccggtgagcg
     2041 gcttgcccga gcgagaaaca ctgcgcccat acaacacggt gaagaacgag ctcctgctcg
     2101 tggcggggct gtggtcggag ctcgcggagc cagaggcgct gccgagagca cgcttcacgg
     2161 tagtttgtga ttaccacgtg acctcggtga tcaagtcggc ggagctccgc tgggcgcctt
     2221 ccggtaagtt cgaccgactc gaccggagcg tgcccgtcgg tttcgtcaag aactgccaaa
     2281 tcccg
//
LOCUS       DASTKS010000314         8388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1836582, whole genome shotgun sequence.
ACCESSION   DASTKS010000314 DASTKS010000000
VERSION     DASTKS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836582"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFQ35_06490"
     CDS             <1..385
                     /locus_tag="VFQ35_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930313.1"
                     /translation="ATFRAAAERRAQGLILAARRSHKLEAGASAVEEAVQHRRAALVL
                     VATDARASADLGWLSPLIASGQALAFSTKATFASWLARPDTALVAITDARIARHAQQA
                     IHWTMLEGPRAPTTGARRTASSEAG"
     gene            382..3237
                     /gene="infB"
                     /locus_tag="VFQ35_06495"
     CDS             382..3237
                     /gene="infB"
                     /locus_tag="VFQ35_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9930314.1"
                     /translation="MTVKMRVYEVARDLGMDNKALVALLQSVGVTEAKNHMSAIAPEA
                     IDRVKRHLEKQKSPSVVEERIRPTVVKRRAAQKPEGSVAPSQPSAAPPPASAPAPMSS
                     APVSVASVSAPPPSEERREPPKPRSSPVAAPPPVEPKVAPPVVEAAPPPPPPPPPPPP
                     PPPSAEAQAPAPVAVQAPVEVKPAPVVEAPPVKPPVETPAKSVAAAPPVEAPAPTPVI
                     EEPLPPPPPPPPAPAPEVRVAPPAPPPAAKPAVAQAPPPVASPAPAAAAPARPAAAKT
                     GIDVWEGRPGVPMPQVQRGTPTPRRVQYDPKAGAMRGRPGQQPPSGVQGRMTGRGPMR
                     PGQRGNFPPSKGPRVNAPPTTQERSAHKKIVRMEETINLQRLAAEMGVKATEVLMKLM
                     RLGTTGVNINSTLDADTAKIVASEFGWEVEDVAVSEADQLVAAQRIEGETGAEERVHR
                     PPVVTVMGHVDHGKTSLLDQIRKASVAAGEAGGITQHIGAYSVPTSHGPVTFLDTPGH
                     EAFTAMRARGAQATDIVILVVAADDGVMPQTREAVNHAKAAKVPIVVAVNKIDKPQAD
                     PERVKRQVSELGLVPEEWGGDTLFCNVSAHTREGVDNLLEQLALQSEVLELKANPSKA
                     ATGIVIEAELDRGRGPVATILVTDGTLNRGDVILAGGAYGKVRAMLDSSGNQVTSAGP
                     STPVAIIGLSDVPSAGDPVHMIRDIKKAQEIAESRKTKEKRSLAPSTGARAMSLEDLH
                     KALAENDQLELKLIIKADVQGSVEAVSDSLTKLSTEKVKVSVVHAGAGAITEGDVNLA
                     VAAGAIIIGFNVRPAGKAAALAQKENIQIRQYSIIYNVVDEVKAAMEGLLAPTIVEKN
                     VGKAEIRQVFRVSKAGNVAGCMVTEGVVRRSSIIRLVRDGENIWSGKLSSLKRFKDDA
                     REVKEGFDCGMAFEGFSDFKEGDVVEAYDTEEVRQTL"
     gene            3262..3660
                     /locus_tag="VFQ35_06500"
     CDS             3262..3660
                     /locus_tag="VFQ35_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET9930315.1"
                     /translation="MFIGVSRIVLTIPGARSLKDKRRVVHAFRDRVRARLSVSIAEVG
                     ELELYQRATLGVAVVARDSQHCSSLLSDVERAARGLREAWVGDVATEVIPMGPGGASL
                     GPGIEQSLSRSELGDDESASDEQELDEDEP"
     gene            3657..4031
                     /gene="rbfA"
                     /locus_tag="VFQ35_06505"
     CDS             3657..4031
                     /gene="rbfA"
                     /locus_tag="VFQ35_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9930316.1"
                     /translation="MSSQDGRRSLRVAELARAEFTRAVLRRIDDPRLRSVMVANVSVS
                     DDLSVVDIGVRFATVEDPVEQQKLVKKLARALPRLTREIVPRLELRRVPTIRLHHDAG
                     EDRTRRVEELLREIHEESKDKP"
     gene            complement(4083..4961)
                     /locus_tag="VFQ35_06510"
     CDS             complement(4083..4961)
                     /locus_tag="VFQ35_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9930317.1"
                     /translation="MELRHLRVFLALADELHFGRAANKLRIAQSAVSQTLMDLEAELG
                     VELIARTKRSVALTGSGRQFIDYARGALTQVEQGALAARHGATGEEGELRLSFTLMST
                     LTPLPRVIAKFRSRYPRVRLSISQGGSVEQLEAIRQGRCDIGFMAFKRDIEPLATEVI
                     TRNALVVVLPSDHALARRRRLELGALAKEPFVFLKQRSEPEVYTRFLGQCARAGFEPN
                     VILEVEHLEALLSFVAAGLGVSCTPNLIERLSFPGIKTVPLSPEIRGGISAVWRPERL
                     PATALRFLEALRAELG"
     gene            5081..6049
                     /locus_tag="VFQ35_06515"
     CDS             5081..6049
                     /locus_tag="VFQ35_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930318.1"
                     /translation="MRAFTSWAALVVLACDARVCPDESGRATELPARLSQTGLFADSS
                     LEQVAPGVFSYLPEFTLWSDGAEKRRWVFVPAGSHVDTSDADDWSFPQGTKFWKEFSV
                     AGRRIETRLLEKFGPQPADWAAVAYIWRADLSDAERSPYGALDVLGTEHDVPAAGECF
                     ACHSGRRDRVLGFSAIQLAPRSYDSGERLSREASEALFSEPVPTLDVPGTPGERAALG
                     YLHANCSHCHNLSDAAKSSGKCLNPTRSLSFDLDFTLHTEELATVERTAARRTAVGKV
                     IRARRPDESKVVELMSKRGGSQMPPLGTEHVDDAGLEIIRAWILGL"
     gene            6031..6918
                     /locus_tag="VFQ35_06520"
     CDS             6031..6918
                     /locus_tag="VFQ35_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930319.1"
                     /translation="MDPRFVRLSAGGFLVVALAFPARAAAEDERLVLSVEARVGTATA
                     PYVTRFFPEVSGYGVALQAFAFWRPASLVGLWFRAPYALMRVEQPAGAFYDEAAFGNP
                     ELGVRVERPLLAVDASDGRAGLDFSIGLPLAEHDSRDSQLEGRALTLANAFSGLSEPE
                     LYTPGVVALTPSAFATLRWSRLSLRGALKIPLLARVSRASLPSDANARSFGMASVVEV
                     AGTVSPLTWLSLSLAPRLTVRERAVVTDGAGPANFLVSGVVGVAPSKGFSAALSAHVP
                     VAGSLRSTLAGGLLLEAKF"
     gene            6966..7571
                     /locus_tag="VFQ35_06525"
     CDS             6966..7571
                     /locus_tag="VFQ35_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930320.1"
                     /translation="MPVARKRALELALSLENASSYPHFDRVAVRTPRRTFATFALRGD
                     DVNFAFDLALQEQFCSLAPDAISPVPGGWGRMGWTSCDLSKVDAATFKAALLAAHARS
                     NVPRGKRTRSAIAKPAAPESKASSREPEPTLSKASRGNKLESPRAKAKAPKAVSSESP
                     RAKAKAPKAVTSESSRGEGKAPRKAASSESPRAKAKAPEQR"
     gene            complement(7632..>8388)
                     /locus_tag="VFQ35_06530"
     CDS             complement(7632..>8388)
                     /locus_tag="VFQ35_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9930321.1"
                     /translation="AEGAQRSAARPATPPPAPGMPPTSSRDVVVTVQCELESLPKLGT
                     VVGILTDGESNGPVAGARIRATDRKNRELELAGDAAGAFRITNIPPGHLKLTIEASGY
                     FPTTTEVDVKALDELQTRIVLNKRPAQPNVVVQGREVKLRKQVHFATDSADIMPDSMA
                     LLEEIADVLNQHTEIPQVEVQGHTDNTGTAPHNLRLSQERAQAVVDALVRLRVDPSRL
                     TAKGYGQEKPIAANASEAGRARNRRVQLIIQGK"
BASE COUNT         1379 a         2823 c         2800 g         1386 t
ORIGIN      
        1 cgccacgttc cgcgccgcgg cggaacggcg agcgcaaggt ctcatcttgg cggcgcggcg
       61 ctctcacaaa ctcgaggcag gcgcgagcgc tgtcgaagag gcggtgcagc accgccgcgc
      121 ggcgctcgtc ttggtggcca ccgatgcgcg cgcttcggcg gacctcggct ggctgtcgcc
      181 gctgatcgcg agcggccagg cgctcgcatt ttctacgaag gcgacgttcg ccagctggct
      241 cgctcggcca gacaccgcgc tcgtcgcgat tacggacgca cgaatcgcgc gtcacgcaca
      301 gcaagcgatt cattggacga tgctcgaagg gcctcgcgct ccaacgacgg gggcacggcg
      361 gaccgcttca tcggaggctg gatgactgtc aagatgcgtg tctacgaagt agcccgcgat
      421 ctcgggatgg ataacaaggc actggtcgcg cttcttcagt ccgtaggggt gaccgaggcg
      481 aagaaccaca tgagcgcgat tgcgcccgag gcgatcgacc gcgtgaagcg ccacctcgag
      541 aagcagaagt ctccgagcgt cgtcgaagag cggatccgcc caaccgtcgt caagcgtcgc
      601 gccgcgcaga agcccgaagg gtccgtcgcg ccctcacagc cgtcggcggc tcctccgccc
      661 gcttccgcac ccgcgccgat gtcttcggcg cccgtgtctg tggcgagtgt cagcgcccca
      721 ccgccctccg aggagcgacg agagccgccg aagccgcgct cgtctccggt tgccgctccg
      781 cctccggtcg agccaaaggt ggctccgccg gtcgtcgaag cggcgccgcc gcccccaccg
      841 ccaccgcccc ctccgccgcc cccaccacct tcggcggaag ctcaagcccc tgcgcccgtt
      901 gccgttcaag cacccgtcga ggtgaagccg gcgccggtcg tcgaggctcc tcccgtcaag
      961 ccgccggtcg aaacgccggc aaaatctgta gcggccgctc cgcccgtaga agcgcccgcg
     1021 ccaaccccgg taatcgaaga gcccttgcca ccacctccgc ctcctcctcc cgctcctgca
     1081 cccgaagtcc gcgttgcgcc gccggcgcct ccgcccgctg caaagcccgc ggtcgctcaa
     1141 gctccgccgc cagtagcttc accggccccg gctgccgcag ccccggctcg tccggccgcc
     1201 gccaagacgg gcatcgacgt ttgggaaggc cgccccggtg ttcccatgcc gcaggtgcag
     1261 cgcggcacac ccaccccgcg tcgcgttcag tacgatccca aggcaggtgc gatgcgtggc
     1321 cgtcccggtc agcagcctcc gtcgggcgtg cagggacgca tgaccggccg tggcccgatg
     1381 cggccgggcc aacgtgggaa cttcccgccg tccaaggggc cgcgcgtcaa cgcgccccca
     1441 acgacgcaag agcgctccgc gcacaagaag atcgtgcgca tggaggagac catcaacctc
     1501 cagcgcctcg ccgccgaaat gggcgtgaag gcgacggaag tgctgatgaa gctcatgcgc
     1561 ctcggcacga cgggcgtgaa catcaacagc accctcgacg cggacaccgc gaagatcgtc
     1621 gccagcgagt tcggttggga ggtcgaagac gtcgcggtga gcgaagccga ccaactcgtg
     1681 gcggcgcagc gcatcgaagg tgagacaggc gccgaagagc gcgtgcaccg gccgccagtc
     1741 gtgaccgtca tgggtcacgt cgaccacggc aagacgagcc tactcgacca gattcgcaag
     1801 gcgtcggtcg cggcgggcga ggccggcggc atcactcagc acatcggcgc gtactccgtg
     1861 ccgacatcgc atgggccggt gacgttcctc gacacgccag gtcacgaggc gttcacggcg
     1921 atgcgtgcgc gcggcgctca ggcaaccgac atcgtgatct tggtggtcgc ggcggacgac
     1981 ggcgtgatgc cgcagacgcg cgaagccgtg aatcacgcga aggcggccaa ggtgccgatc
     2041 gtcgtcgccg tcaacaagat cgacaagccg caagccgacc ccgagcgcgt caagcgtcag
     2101 gtgagcgagc tcgggctcgt gccggaagag tggggtggcg acacgctgtt ctgcaacgtt
     2161 tccgcgcaca cccgggaggg cgtcgacaac ctcctcgagc agctcgcgct gcagtccgag
     2221 gtgctcgagc tcaaggcgaa cccgagcaaa gccgcgaccg gcatcgtcat cgaagccgag
     2281 ctcgatcgcg ggcgcggtcc cgtcgccacc atcttggtga ccgacggcac cctgaaccgc
     2341 ggcgacgtca tcctcgcggg tggcgcgtac ggcaaggtcc gcgcgatgct cgactcgtct
     2401 ggcaaccagg tgacgagcgc cgggccttcg acgcccgtcg ccatcatcgg cttgagcgac
     2461 gtgccgagcg cgggtgaccc cgtgcacatg atccgcgaca tcaagaaggc tcaagagatc
     2521 gccgagagcc gcaagaccaa agagaagcgg agcctcgccc cgtcgaccgg tgcgcgcgcg
     2581 atgtcgctcg aggacttgca caaagcgctc gccgaaaatg atcagctcga gttgaagctc
     2641 atcatcaagg cggacgtgca gggctcggtc gaagccgtct ccgactcgct caccaagctc
     2701 tccaccgaga aggtgaaggt cagcgtggtg cacgccggtg cgggcgccat caccgaaggc
     2761 gacgtaaacc tcgccgtcgc ggcgggtgcc atcatcatcg gcttcaacgt gcgccccgcg
     2821 ggcaaggccg ccgctctcgc ccaaaaggag aacatccaga tccgccagta cagcatcatc
     2881 tacaacgtcg tggatgaggt gaaggcggcg atggaaggtc tgctcgcgcc gacgatcgtc
     2941 gagaaaaacg tcggcaaggc ggagattcgt caggtgttcc gcgtcagcaa ggcgggcaac
     3001 gtggcgggct gcatggtcac cgaaggtgtc gtgcgccgct cctcgatcat ccgcctcgtg
     3061 cgcgacggcg agaacatctg gtcgggcaag ctctcgagcc tcaagcgctt caaagacgac
     3121 gcgcgcgagg tcaaagaagg cttcgactgc ggtatggcgt tcgaaggctt ctccgacttc
     3181 aaggaaggcg acgtggtcga ggcctacgac accgaagagg tgcgccagac gctgtagcgc
     3241 cgcacggcgc tcacgaaccg catgttcatc ggagtatccc gcatcgtgct cacgattccg
     3301 ggggctcgtt cgctgaagga taagcggcgc gtggtgcatg cttttcgcga tcgagtgcgc
     3361 gcgcgcctca gcgtctcgat agcggaggtc ggcgagctcg agctctatca acgggcgacc
     3421 ctcggggttg cggtggtcgc ccgggactcg cagcactgct cgtctcttct gtcggacgtc
     3481 gaacgcgcgg cccgcggcct gcgcgaggcg tgggtcggcg acgtggcgac cgaggtgatt
     3541 ccgatgggcc cgggcggtgc ttcgctcggg cccggcatcg agcagagcct ctcgcgctcc
     3601 gagctcggcg atgacgagtc cgcgagcgac gaacaagaac tggacgagga cgaaccatga
     3661 gctcgcaaga tggtcgacgg agcttgcgcg tagccgaact cgcgcgcgct gaattcaccc
     3721 gcgcggtgct gcgccgcatc gacgatccgc ggttgcgcag cgtgatggtg gccaacgtct
     3781 ccgtctcgga cgatctctcg gtggtcgaca tcggggtacg cttcgccacc gtcgaggacc
     3841 ccgtcgagca gcaaaagctc gtgaagaagc tcgcgcgggc cctgccccga ctcacgcgcg
     3901 aaatcgttcc acgcctcgag ctccggcggg tgcccaccat ccggcttcat cacgacgccg
     3961 gtgaagatcg cacccgccgc gtcgaggagc tcttgcgcga gatccacgag gagtcgaagg
     4021 acaagcctta gcagtgcgaa atccgagatc ggagggagcc cgaactggct ctcggacagc
     4081 cctcaaccga gctcggctcg cagtgcttcg aggaagcgca aagcggtcgc tggcaggcgc
     4141 tcgggtcgcc acacggcgct gattcctccc cggatctccg gtgacagcgg caccgttttg
     4201 atgccgggaa agctgagtcg ttcgatcaag ttcggggtgc aggagacgcc cagccccgcc
     4261 gcgacgaagc tcaacaaagc ttcgaggtgt tcgacctcca aaatcacgtt gggctcgaag
     4321 ccggcgcggg cacactgccc gaggaagcgc gtgtagacct ccggctcgct ccgctgcttc
     4381 aaaaagacga agggctcctt ggcgagcgcg ccgagctcca ggcggcgccg ccgcgcgagc
     4441 gcgtggtccg atggcagcac gacgacgagc gcattgcgcg tgatgacttc ggtcgcgagc
     4501 ggctcgatgt cgcgcttgaa ggccatgaag ccgatgtcac agcgaccctg ccggatcgcc
     4561 tcgagttgct cgacgctgcc gccttggctg atgctcaagc gaacgcgggg atagcggctt
     4621 cgaaacttcg cgatgacgcg tggcaacggg gtgagcgtgc tcatcagggt aaagctcagc
     4681 ctgagctcac cttcttcccc cgtcgccccg tgacgcgccg cgagcgcgcc ttgctccacc
     4741 tgggtgagcg cgccgcgcgc gtagtcgatg aactgccgtc cggagccggt gagcgcgacg
     4801 ctgcgcttcg ttcgcgcgat gagctccacg ccgagctccg cctcgagatc catcagcgtt
     4861 tggctcactg ccgattgtgc gattcgcagt ttgtttgcgg cgcggccgaa gtggagttcg
     4921 tcggcgagcg ccaaaaatac gcgtaagtgg cgaagttcca tcggttatcg cagttagaga
     4981 ttactcttgt aataataatc tattggacga gtctcgcgcc agactgcatt gtccggagtg
     5041 aaacgaagac gttcgccgtc tttcttcacc ccacatgtgg atgcgagcct tcacgagctg
     5101 ggcggcgctg gtcgtccttg cctgcgacgc gcgcgtgtgc ccggacgagt cgggtcgcgc
     5161 caccgaattg ccagcgcgcc tttcccaaac cggtctgttc gcggactcct ccttggagca
     5221 ggtcgcgcct ggcgtcttct cgtaccttcc cgagttcacg ttgtggagcg acggcgccga
     5281 aaagcgtcgc tgggtcttcg tgccggcggg cagtcacgtc gatacgagcg acgccgacga
     5341 ctggagcttt ccgcagggca cgaagttttg gaaagagttc agcgtcgcgg ggcgccgcat
     5401 cgagacgcgc ttgctggaaa agttcgggcc gcagccggca gactgggcgg cagttgcgta
     5461 catttggcgc gcggatctca gcgacgccga gcggtcgccc tacggcgcgc tcgatgtgct
     5521 cggaaccgag cacgacgtgc cggctgcggg tgagtgcttc gcctgtcaca gcgggcgccg
     5581 cgaccgcgtc ctcggctttt cggcgatcca gctggcgccg cgctcgtacg attcgggcga
     5641 gcgcttgtcg cgcgaggcga gcgaagcgct cttctccgag cccgtcccaa cgctcgatgt
     5701 tccgggcacg cccggcgagc gcgccgcgct cggctatttg cacgcgaatt gctcgcattg
     5761 ccacaatctc tccgacgcag ccaaatcgag cgggaaatgc ctgaacccga cgcggagctt
     5821 gagcttcgat ctcgacttta cgttgcacac ggaggagctc gccaccgtcg aacgaaccgc
     5881 cgcgcgtcgc accgcggtcg gaaaggtgat tcgcgcgcgg cgccccgatg aaagcaaagt
     5941 cgtggagctc atgtccaagc gtggcggctc gcaaatgcct ccgctcggaa cggagcacgt
     6001 cgacgacgcg ggcctggaga ttattcgcgc atggatcctc ggtttgtgag actcagcgca
     6061 ggcggctttt tggtcgttgc tctcgctttt ccggctcgcg cggcggccga ggacgagcgt
     6121 ctggttctca gcgtcgaggc gagggtaggg accgccaccg cgccgtacgt gacccgcttt
     6181 tttccggaag tgagcgggta cggtgtcgcg cttcaggcgt tcgcgttttg gcggccagcc
     6241 tctttggtgg gcctctggtt tcgtgcgccg tacgcgctca tgcgcgtcga gcagcccgcg
     6301 ggcgcctttt acgacgaagc ggccttcggt aacccggagc tcggcgtccg cgtggagcga
     6361 ccgttgctcg ctgtggatgc tagcgacggt cgcgccggcc tcgatttctc gatcgggctc
     6421 cctcttgccg agcacgattc acgcgactcg caactcgaag ggcgagccct cacgctcgcg
     6481 aacgcattct cgggcctctc cgaaccggag ctctacacgc cgggtgtggt cgcgctgacc
     6541 ccttcggcct ttgcgaccct gaggtggtcg cggctatctt tgcgtggtgc gctcaagatt
     6601 ccgttgctcg ctcgagtttc ccgcgcgagc ctcccttccg acgcgaacgc gcgctcgttc
     6661 ggcatggctt ccgtcgtcga ggtggcgggc accgtgtcgc cgctcacctg gctgagtcta
     6721 tcgctcgcgc cgcgcctcac cgtccgcgag cgcgcggtag tgacagatgg cgctggcccg
     6781 gccaatttcc tggtttcagg ggtggtcggc gtggcgccgt cgaaggggtt cagcgccgct
     6841 ctctcggcgc acgtccccgt cgccggctcg ctccgctcga cgctggccgg aggcttgctc
     6901 ctggaagcga agttctgagc gcccgcggtt accggctcga cgacggccgc tgtaagctcc
     6961 cgctcatgcc cgttgcccga aagcgcgccc tcgagctcgc tctgtcgctc gaaaacgcga
     7021 gctcgtatcc acatttcgac cgcgtcgcgg tgcgcacgcc acgccggacc ttcgccacgt
     7081 ttgctcttcg tggcgacgac gtcaacttcg cgttcgacct cgcgctgcag gaacagtttt
     7141 gttcactcgc gcctgacgct atctcaccgg tgcccggggg ctggggacgc atgggatgga
     7201 cgagctgcga tctgagcaag gtcgacgcgg cgaccttcaa ggctgctctc ctcgccgcgc
     7261 acgcccgctc gaacgttccg cgcggcaagc gcacacgctc ggcgatcgcc aaacccgccg
     7321 cgcccgagag caaagcctcg agccgcgaac ccgagccgac tctttccaaa gcatcaaggg
     7381 ggaacaagct cgagtcgccg cgggccaaag cgaaggcgcc aaaggcagtg agttccgagt
     7441 cgccgcgggc caaagcgaag gcgccaaagg cggtgacttc cgagtcgtcg cgaggcgagg
     7501 ggaaggcgcc gaggaaggca gcgagttccg agtcgccgcg cgccaaagcg aaggcgccgg
     7561 agcagcgctg agcgatcgac tcccgcgctg ctcggagctc ggaacagcgc gggtgctttc
     7621 gatgctggct actacttgcc ttggatgatg agctgcacgc gccgattgcg cgcgcggcct
     7681 gcctcggaag cgttcgccgc gatcggcttt tcttgtccgt aacccttggc cgtgagacgg
     7741 ctgggatcga cgcgcagtcg cacgagtgcg tcgaccaccg cctgcgcgcg ctcttggctg
     7801 agtcgcaggt tgtgcggcgc tgtaccggtg ttgtcggtat ggccttgcac ctcgacttgg
     7861 gggatttcgg tgtgctggtt cagcacgtcc gcgatctctt cgagcagggc catcgagtcg
     7921 ggcatgatgt ccgcggagtc ggtcgcaaag tgaacttgct tgcgcagctt cacctcgcgg
     7981 ccttgaacga cgacgttcgg ctgcgcggga cgcttgttga gcacgatgcg cgtttggagc
     8041 tcgtcgagcg ccttcacgtc gacctcggtg gtcgttggga agtatccaga cgcctcgatc
     8101 gtgagcttca agtgcccggg cgggatgttc gtgatgcgga aggcgcccgc tgcatccccg
     8161 gcgagttcga gctcccggtt cttgcgatcg gtcgctctga tgcgcgcccc ggcgacgggt
     8221 ccgttcgact cgccgtcggt gaggatgccc acgaccgtgc cgagcttcgg caacgactcg
     8281 agctcacact gcaccgtcac gacgacgtca cgcgagctcg tcggaggcat gccaggagct
     8341 gggggcggtg tcgccggccg cgctgcagag cgctgagcgc cctctgcg
//
LOCUS       DASTKS010000315         5511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1166863, whole genome shotgun sequence.
ACCESSION   DASTKS010000315 DASTKS010000000
VERSION     DASTKS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166863"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFQ35_06535"
     CDS             <1..319
                     /locus_tag="VFQ35_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HET9930322.1"
                     /translation="TSEYGRQCKAWGGRDLWHMRSLLATEKVDLLIGNTYGKYLERDI
                     GTPLIRLAFPIFDRHHHHRFPTWGYQGGLRVLVTVLDKIFEKIDVDTIIPAITDYSYD
                     ITR"
     gene            459..1895
                     /gene="nifE"
                     /locus_tag="VFQ35_06540"
     CDS             459..1895
                     /gene="nifE"
                     /locus_tag="VFQ35_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HET9930323.1"
                     /translation="MADALKDKIADVFNEPGCEKNQQKGAKERKKGCSKPLTPGAAAG
                     GCAFDGAKIALQPITDVVHLVHGPLACEGNGWDNRHASTSGSSLYRRGFTTDISEVDI
                     VMGNGEKRLYRAIKEAVQKHSPPAVFVYSTCVTALIGDDIDAVCKHATQKFGVPVVPV
                     NAPGFVGAKNLGNKLAGEALLDHVIGTREPELVTPYDVNILGEYNLSGELWQVKPLLE
                     RLGIRLQACITGDARYNDVAASHRASVNMMVCSTALINLARKMEERYGIPYFECSFYG
                     IEDTSTALRTVAKMLVERGAPADLLARTEELVVSEEARAWARIRPYQSRLEGKRVLLY
                     TGGVKSWSVVSALQEIGMVIVGTSTRKSTKEDKERIKELMGGDAHMVDSIPPRQMYDQ
                     LIRGEADILLSGGRTQFVALKARVPWLDINQERHYAYGGYDGMVELVRQLDLEINSPI
                     WSEVRRAAPWEPGFERTEATQTGLAQSA"
     gene            1904..3283
                     /gene="nifN"
                     /locus_tag="VFQ35_06545"
     CDS             1904..3283
                     /gene="nifN"
                     /locus_tag="VFQ35_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HET9930324.1"
                     /translation="MAEVVASKKSCAENPLKSSTPLGAALAYLGVEGAVPLFHGSQGC
                     TSFALVLAVRHFKEAVPLQTTAMNEPATVLGGTDNLDEALVVIANRMKPKFIGVASTA
                     LVETRGEDFAGGLRTIVEKRRELDGIRVVFASTPDFDGALEDGWAKATSAIIEALVLP
                     KASVSSTQRTVNVLPGVHETPGTLEELRRTLAAFGLAARIVPDLSGSLDGHVPDQYIG
                     TSLGGAALNDISNMADAVHTIAIGEHMRAPAEALSKKTGVPSTVFPTLLGLRASDELV
                     ALLTEVSGVPAPSSIRRQRSQLVDAMLDGHFHLSGKRVAIAADPDLLASLFYFFHGLG
                     ARVEVAIASTKNSPLLATLPCDKVVVGDIGDLEELAKERDCELLVTHSHGNMASEALG
                     IPLFRIGFPIFDRLGHQHRSLGGYVGTRELIYQVANEFMGLIHEHKPEDFASAVPPSL
                     VMDTRDAAP"
     gene            complement(3487..3762)
                     /locus_tag="VFQ35_06550"
     CDS             complement(3487..3762)
                     /locus_tag="VFQ35_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930325.1"
                     /translation="MKHASQLPDFVEHLAERLGLERDAALERLGDWLHRYQPVRRREI
                     RVLQVREVAEPKAPRASFVQESRSRRELRIAENDEASGTRPELARVG"
     gene            3976..4425
                     /locus_tag="VFQ35_06555"
     CDS             3976..4425
                     /locus_tag="VFQ35_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930326.1"
                     /translation="MTLEAPFADNRVILLHFDTYSAALVFARFGKSVLLPEPLPATAV
                     PMTAPSERAAAHEGDEVLRASVSRYGLNPEELGRMRGFDEWVQTDSGPIRIHLLRFTT
                     FLAPAEAIAPHGGVFAPLSELRGIMKVELPFVRQAFNLILGGESSSR"
     gene            4691..4885
                     /locus_tag="VFQ35_06560"
     CDS             4691..4885
                     /locus_tag="VFQ35_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930327.1"
                     /translation="MFIYCNLGESSAALAQNFADLGLTEFDGVDARFNAIWRAFELAV
                     RRLGVTRKGSRDERCPSPAP"
     gene            5030..5341
                     /locus_tag="VFQ35_06565"
     CDS             5030..5341
                     /locus_tag="VFQ35_06565"
                     /inference="COORDINATES: protein motif:HMM:NF039672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HET9930328.1"
                     /translation="MTSVPEGLTCRYFVTYSGARLPVRLVNPIDENELTHRNTFIRAY
                     FDPMERLSRWEKLVYGRVELAHRYSYDENGTLRRVVIMSEDEEQVLEFDAAGRRGLGP
                     S"
BASE COUNT         1018 a         1741 c         1780 g          972 t
ORIGIN      
        1 gacgtcggag tacggtcggc agtgcaaagc ttggggcggc cgcgacctct ggcacatgcg
       61 ctcgctgctt gcgacggaga aggtggacct tctcatcggc aacacgtacg gcaagtacct
      121 cgagcgcgac atcggcactc ccctgattcg cctcgcgttc ccgatcttcg accgccacca
      181 tcaccatcgc ttcccgacct ggggttacca aggtgggttg cgcgtgctgg tgactgtgct
      241 cgacaagatc ttcgagaaga tcgacgtcga caccatcatc cccgctatca ccgattactc
      301 ttacgacatc acccgctgaa gcgagaggcg cggggtgagg ctacgctcct gggcctcact
      361 ccgcgccaac attttctagc tcgctctgcc gacccacttc gactcgaact gcgcggccaa
      421 cgcgccgctc ccgcgaacac cccaccgagg ctccttccat ggctgatgcg ctgaaagaca
      481 agatcgctga cgtgttcaac gagcctggct gcgaaaagaa tcagcagaag ggcgccaagg
      541 agcggaagaa gggctgctcg aagccgctca cgccgggagc ggccgccggc gggtgcgcat
      601 tcgacggagc aaagatcgcg ctccagccga tcaccgacgt cgtgcacctg gtgcatgggc
      661 cgctcgcgtg tgaaggaaac ggttgggaca accggcacgc atcgacttca ggctcgtcgc
      721 tctatcgccg cggtttcacg accgacatca gcgaagtcga catcgtgatg gggaacggcg
      781 aaaagcggct ctatcgcgcg atcaaggagg cggtccagaa gcactcgccg ccggcggtgt
      841 tcgtctattc cacttgcgtc accgcgctga taggggacga catcgacgcc gtgtgcaagc
      901 acgcgacgca aaagttcggc gtgcccgtgg taccggtcaa cgcaccaggc ttcgttggcg
      961 ccaagaacct tggaaacaag ctcgcgggcg aagcgctctt ggaccacgtg atcggcactc
     1021 gcgagcccga gctggtcacg ccgtacgacg tgaacatcct gggggaatac aacctctcgg
     1081 gcgagctctg gcaggtcaag ccgctgctcg agcggctggg gattcggctc caggcgtgta
     1141 ttacgggtga cgcgcgctac aacgatgtcg cggcctcgca tcgtgccagc gtcaacatga
     1201 tggtgtgctc caccgcgctc atcaacctcg ctcggaagat ggaggagcgc tacgggattc
     1261 cgtacttcga gtgctccttc tacggcatcg aggacacctc gacggcgctt cggacggttg
     1321 ccaaaatgct cgtcgagcgg ggagcccccg ctgacctcct cgcccgcacc gaagagctcg
     1381 tggtgagcga ggaagcgcgc gcctgggctc gaatccggcc ataccagagc cgcctcgagg
     1441 gcaaacgggt gctactctat accggtgggg tgaagtcctg gtcggtcgtc tccgccttgc
     1501 aagagatcgg catggtcatc gtcggaacat cgacgcgcaa gtcgacgaag gaagacaagg
     1561 agcgcatcaa agagctcatg ggcggcgatg cgcacatggt cgactcgatc ccgccgcgcc
     1621 aaatgtacga ccagctgatt cggggcgaag cggacatctt gctctctggc ggccgcacgc
     1681 aattcgtcgc gctgaaagcg agggtgccgt ggctcgacat caatcaagag cggcactacg
     1741 catacggagg ctacgacggc atggtcgagt tggttcggca gctcgatctc gagatcaaca
     1801 gccccatctg gtcggaagtt cggcgggcgg cgccgtggga gccagggttc gaacgcacgg
     1861 aagcgacgca aacggggctt gcacagagcg cgtgaggtga cgcatggccg aggtggttgc
     1921 cagcaagaag tcgtgcgcag aaaatccgct gaagtcgtcg acgccgctcg gcgcggcgct
     1981 cgcgtacctc ggcgtagagg gcgcagtgcc gctctttcac ggttcgcagg gctgcacatc
     2041 gtttgccctc gtgctcgcgg tgcgccactt caaggaggcc gtcccgctgc agaccaccgc
     2101 gatgaacgag ccagcgacgg tgcttggtgg caccgacaac ttggacgagg cgctggtcgt
     2161 gatcgcgaac cgaatgaaac ccaagttcat tggggttgca tccaccgccc tcgtcgagac
     2221 gcgcggcgaa gatttcgcgg gtgggcttcg aaccatcgtc gaaaagcggc gcgaactcga
     2281 cggtattcgc gtcgtgttcg cctcgacgcc cgacttcgat ggcgccctgg aagacggctg
     2341 ggccaaggcg acgagcgcga tcatcgaagc gctagtcctc ccaaaggcga gcgtcagcag
     2401 cacgcagcgc accgtcaacg tgcttccggg cgttcacgag acccccggca cgctcgagga
     2461 gcttcgccgc acgctcgcgg cgttcgggct tgctgcgcga atcgtgccgg acctttccgg
     2521 gtcgctcgat gggcacgtcc cggatcagta cattggcacg agcctcggcg gcgccgcgct
     2581 caacgatatc tccaacatgg ctgacgcggt gcacacgatc gccattggcg aacacatgcg
     2641 cgcaccagcc gaggcgttga gcaaaaagac gggggtgccg agcacggtgt tcccgacctt
     2701 actcggcctc cgcgcgagcg acgagctcgt cgccctcctc accgaagtgt cgggggtgcc
     2761 cgcgccgagc tcgattcggc gccagcgcag ccagctcgta gatgccatgc tcgacgggca
     2821 ctttcacctg agcggcaagc gtgtggcgat cgcggccgac ccagacctgt tggcttcgct
     2881 cttctacttc ttccacgggc tcggcgctcg tgtcgaggtc gcgattgcct cgaccaagaa
     2941 tagcccgctg ctcgcgacgc tgccttgcga caaggtagtc gtcggagaca ttggtgatct
     3001 cgaagagttg gcgaaagagc gcgactgcga gctgctcgtc acgcatagcc acggcaacat
     3061 ggcttctgaa gcgctcggca ttccgctctt tcgcattggc ttccccatct tcgatcgatt
     3121 ggggcatcag caccgaagcc tcggtggtta cgttggaacc cgggagctca tctatcaagt
     3181 agccaatgag ttcatggggc taatccacga gcacaagcca gaggacttcg cctccgcggt
     3241 tccgccgtca ctcgtcatgg atacgcgcga cgccgcgccg tgaggagatc gacacgagtc
     3301 acgctgccgg cgatgcgcca ccccgggcca aaatgactgg ccgtaacccg cgccacgcgc
     3361 gacgtcctcg gcgcgcagcc ctgagcaaac ggggttcgcg actccgcgcc gtgccgtgag
     3421 caaccggggt tcgcgactac cgcttccccc cgcggagagc gacgtcttgc gtcagccagc
     3481 gcggagctac cccactcggg cgagctcggg tcgcgtgccg ctcgcctcgt cgttctcggc
     3541 tattcggagc tctcgtcgcg agcgcgactc ctgcacgaac gacgcgcgcg gagccttcgg
     3601 ttcagctacc tcgcggactt ggagaacgcg gatttcacgg cggcggacag gctgataacg
     3661 atgcagccag tcacccagcc gctcgagtgc cgcgtcacgt tccaagccga ggcgctcggc
     3721 taggtgctcg acgaagtcgg gaagttggct tgcgtgcttc attgcagtca cctttcggtg
     3781 caggcctacg ccgagccgag atgtgcaagg tgtgtgcctc ttggggatac gcgtaaaatc
     3841 ggggagccgg gcgcagcgcg acaattcacg tgtccgacat gcgacaaacg cgaggccatc
     3901 gcgcggccgt ctgctcgcta ttcgaccaaa tcgagggccg ctcgctttgg catgttcgtt
     3961 gcgaagttcc gagacgtgac gttagaggct ccgttcgctg ataatcgggt catcctgctg
     4021 cacttcgaca cctatagcgc cgcgctcgtt tttgctcgct ttggtaagtc ggtgttgttg
     4081 ccagagccac tgcccgctac ggccgtccca atgaccgcgc cgagtgagcg agcggccgcc
     4141 cacgagggtg atgaagtcct tcgagctagt gtgagccgct atgggctcaa ccccgaagag
     4201 ctcggccgca tgagaggctt cgacgagtgg gttcaaaccg actcggggcc gattcggatc
     4261 cacctgctgc ggtttacgac cttcctcgcg cccgcggaag cgatcgctcc gcacgggggc
     4321 gtcttcgccc ctctcagcga gctccggggc atcatgaaag tcgagcttcc gttcgttcga
     4381 caggctttca atctcatcct gggtggtgag tcgagcagcc gttgaaacgg ccgtttcgca
     4441 aggagtgacg cggtgggcgg tgccatgaag aagtcaattc acgatctgaa cagctgagat
     4501 gtccgttcca aaggctcacc gattacgcgc tgagcaggcg ctcaggctca ttcttcaggc
     4561 tgcgcgcagc gagaggtcga gcgcgggaag cgagcccgag gcacacagcg gcgacgaccg
     4621 cgacagggag caggaaagcg ggctctctga gggcgaatcg caggccgaga cgcaaccgcg
     4681 ttcgctccca gtgttcattt actgcaatct cggcgagtcc agcgcggcgc tggcgcagaa
     4741 ctttgcagat ctcggcttga ccgaattcga cggcgtcgac gcgcggttca acgcgatctg
     4801 gcgggccttc gagctcgcgg tgcgccgact tggagtgacg cgcaaaggct ctcgcgacga
     4861 gcgctgcccg tcgccggccc cgtgaggttc ggcgcgcgcc cgtcgagcgc gtaggcccac
     4921 ttcgaaacaa ggccagtgta acgcggtggt gctgccacac cgacgatcgt catatcgaat
     4981 gactcggcat cttgcgaatg agaatgcatc gggcgaggct cgcgagtgca tgacctccgt
     5041 ccccgaaggc ctcacgtgcc gatatttcgt gacctacagc ggagcccgtt tgccggtacg
     5101 gctcgtcaat ccaatcgacg agaacgagct cacgcaccgc aatacgttca ttcgcgcata
     5161 cttcgacccg atggagcgct tgagccgttg ggagaagttg gtctatgggc gtgtcgagct
     5221 ggcgcaccgg tacagttacg acgagaacgg cacgctgcgg cgtgtcgtca tcatgtcgga
     5281 ggacgaggag caggtgctcg agttcgacgc tgcagggagg cgcggtctcg gcccttcatg
     5341 aacgagtttc gacggttgtc cgaaacctca cagtggcgcg aatgcgacgt cgcgacggag
     5401 ccccccagag cgacctgttt gggcaaagcg acgtggtttg gtgacggcgg cccttggggc
     5461 acgtggtttg cttccggcgc ggggcgacgc ggcgagcttg caacgctgcg c
//
LOCUS       DASTKS010000316         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_720004, whole genome shotgun sequence.
ACCESSION   DASTKS010000316 DASTKS010000000
VERSION     DASTKS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720004"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3135)
                     /locus_tag="VFQ35_06570"
     CDS             complement(<1..3135)
                     /locus_tag="VFQ35_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930329.1"
                     /translation="MFKVECPGCKAPYQVDERRIPQSGLKMRCPKCGSSFKVESPSEA
                     QRPAPPPVLGAVLGVTSDSLVPPPEPAAAPKGASALKATMLGVAPAAPQAPRPAAPPA
                     PPRPKASSHADEDLPSPVDRNAADLPSPAVRKGPPPAPPRGKPPAPPAPAPSKKVESE
                     PPLEVEDFTSEPPDLPATAAKAPPRPAHADLPAKAAPRAEPFAPRASATAQTRPAFEK
                     PQLPQAAAPGATSDLPNLAGTSNKARAGFGEELDLPSLSGSGPSGGGAPKGAAPARPT
                     QRSFGDIELDLPDAALRGRRSQPELDLDLPDVSKQAPRASTRSSAPEFDLPSLQHGFD
                     LPSPADNLPALGELPSISNAGLPAVSQGALPALGQGTLPSLSQGGLPSVSSIPPAIGM
                     PAGTPHEARPDPISLPPLELDGPPQPSPRRSFGELDLEGLGPSPARTPSGARPAMSSR
                     PDDSLEADPFGEADLPPPRSLSGASAQSAPRAAARFSSGPPAALGAGPAGSAQSPVIR
                     QAGGGTAYGEVNLGEGISDEVALDGASAPRAAMSSEQDMEFGDVPQEPSAPTAAATAP
                     VSSGAAVARPSGPVSRRAADAAAKPAGRKRAFRSLALLGVLVVGGAALAFVPDVGPFG
                     AYFIIDQVKAGQYAELVATTATQTRKALAKDTAPDAKAAAQHIEEVRAASPRVRSLRT
                     FAAYVAVERELRFGADAELHARAKVLLDEFEGREGLPYLTLARAGRAASEGQFDRARQ
                     LLGTAPNDVDALFLRGEIELRARNAAEALATWQKLDAQSPSARTKFGVARAAFTSGDR
                     KLTEQAAKAALAENPAHVGAKILLARLAADSAREAEAIQLLEQVTKAPESASSEERVM
                     SNTTLGDIHLGRSRITRAEAAYTEALKIDPKAARALLGIGEALFRAGRYSEAQARFEA
                     AAQADPSDVAAKVGVAKSKLMLERVDEATSMLKSLRNSDPKSLLIGYWYARALEAVGN
                     RDEAEKVYTQGIAKTSTDPMLVDTYIALAMLQNQQGRAEVARKTLAQAREKLPSSSAI
                     YKALGEVAISQARYA"
     gene            3328..3915
                     /locus_tag="VFQ35_06575"
     CDS             3328..3915
                     /locus_tag="VFQ35_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9930330.1"
                     /translation="MQRTTAIAVGLLFVLGIVLVLVMGRSSPHAATATHPAPSASAPP
                     SAAPVASLAAPSVPSAASSADAVELDEIKQSEGFDTLPDGRKVPELPANAPKEVSFGV
                     IQVAYQGAQSAPKDARSKADAKTRASTLAAEAKQDFAAAVAKGDRGSTSDAGRVPRDV
                     LEPVVEYLLFTLPKGEVYPEPIDTPRGYWVIRRNR"
     gene            4092..>4515
                     /locus_tag="VFQ35_06580"
     CDS             4092..>4515
                     /locus_tag="VFQ35_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930331.1"
                     /translation="MSSTSTQAPPRSSTPDERALTSSIERLRAEHDANDNGALQSVLL
                     HEMGVLAERSGDEAAAARDYLAAVNIDPEFREPLERLVAIIERRQSYRNLGKVLERLT
                     RVADTADERARSLSDQAAFLADQQNELDAARRCLEEAVE"
BASE COUNT          688 a         1526 c         1562 g          739 t
ORIGIN      
        1 tgcatatcgg gcttgggaga tcgcgacttc gccgagtgcc ttgtagatgg cgctcgagct
       61 cggcagtttt tcacgcgctt gggccagcgt cttgcgagct acctcggccc gtccttgctg
      121 gttttgcagc atcgcgagcg caatgtatgt atcgaccagc attgggtccg tcgacgtctt
      181 cgcgatgccc tgggtataga ccttctccgc ttcgtcgcgg ttgccgacgg cctcgagagc
      241 gcgagcgtac cagtagccga tcaggagcga cttcgggtcg gagttacgca gcgacttcag
      301 catcgacgtc gcctcgtcca cgcgctcgag catgagcttg ctcttcgcaa cgccaacctt
      361 cgccgccacg tcgctcggat cggcttgggc cgcggcctcg aagcgagctt gcgcctcgga
      421 atagcgaccg gctcgaaaca gcgcctcgcc gatgccgagc agcgcccgcg ccgccttcgg
      481 gtcgatcttc agtgcctcgg tatacgcggc ctcggcgcga gtgatgcgcg agcgccccag
      541 gtggatgtcg ccgagagtcg tgttcgacat cacgcgctct tcgctgctgg cgctctcggg
      601 agccttggtg acctgctcga ggagctgaat tgcttccgct tcgcgagcac tgtcggcggc
      661 caggcgcgcc agcaagatct tcgcgcccac gtgcgcgggg ttctcggcga gcgcggcttt
      721 ggcggcttgt tcggtgagct ttctgtcgcc gctcgtgaac gcggcgcggg cgactccgaa
      781 cttggtgcgc gcgctcggac tctgcgcgtc gagcttttgc caggtcgcga gcgcctcggc
      841 cgcgttgcgc gcgcggagct cgatttcacc gcgcaggaag agcgcgtcga cgtcgttcgg
      901 ggccgtgccg agcagctggc gcgcccggtc gaattggcct tcggacgcgg cgcgaccggc
      961 gcgggccagc gtgaggtatg ggagcccctc tcgtccttcg aactcgtcga ggagcacctt
     1021 ggcgcgcgcg tgaagctccg cgtcggcccc gaatcgcagc tcgcgctcta ccgcgacgta
     1081 cgcggcgaag gtcctcaacg agcgcacccg gggcgatgcc gcgcgcactt cttcgatgtg
     1141 ctgcgccgcg gctttcgcgt ccggcgccgt gtctttggcc aaggccttcc gcgtttgggt
     1201 cgcggtcgtg gccacgagct cggcgtattg tcccgccttc acctgatcga tgatgaagta
     1261 ggcgccgaac ggacccacgt cggggacgaa cgcaagcgcg gctcccccca cgacgagcac
     1321 gccgagtagc gcgagcgaac gaaacgcacg cttgcgacca gccggcttcg ccgcggcgtc
     1381 cgcggcccgt cgcgaaaccg gaccactcgg gcgagccacg gccgcgccgg acgagacagg
     1441 cgcggtggca gccgcggtcg gcgcgctcgg ctcttgcggc acgtcgccga actccatgtc
     1501 ctgctcgctg ctcatcgcgg cgcgcggagc ggaagcgccg tcgagcgcca cctcgtcgga
     1561 gatcccctcg ccgagattga cctcgccgta tgccgttccc ccacccgctt ggcgaatcac
     1621 cggcgattga gcgctgccgg ctggcccggc gccgagcgcc gcgggcggcc ccgacgagaa
     1681 acgcgcggct gcgcgcggag cactttgagc agaggcgccg ctcaacgagc gaggaggagg
     1741 gagatctgct tcgccgaacg ggtcggcctc gagcgaatca tcgggccgcg acgacatcgc
     1801 cgggcgcgct ccgctcggag tccgagcagg actcggcccg aggccctcca agtcgagctc
     1861 cccgaacgaa cgccgcggag acggctgcgg gggcccgtcg agttcgagcg ggggcaagct
     1921 aattgggtcc ggacgcgcct cgtgcggcgt tccggcgggc atgccaatcg ccggggggat
     1981 cgagctcacg ctcggaagcc ctccctgact gagcgacggc aaggtgccct ggccaagggc
     2041 ggggagcgct ccctgactca cggcggggag acccgcgtta ctgatgctcg gcagctctcc
     2101 gagggcggga agattgtcag ccggactcgg caaatcgaag ccgtgttgaa gactcggcag
     2161 gtcgaattcg ggcgcgctcg agcgcgtgct cgcacgcggc gcttgcttcg aaacgtcggg
     2221 cagatcgagg tcgagctccg gttgactgcg acggcctcgg agtgccgcgt cgggcaagtc
     2281 gagctcgatg tccccgaagg aacgctgcgt cgggcgagca ggagccgcgc ccttcggagc
     2341 gccgccaccc gatggccccg agccggacag cgagggcaag tcgagctcct caccgaaccc
     2401 agctcgcgcc ttgttggagg tgcccgcgag gttcggcaag tcagaggtcg cgcctggcgc
     2461 ggccgcctga ggcagttggg gcttttcgaa tgcgggccgc gtttgcgccg tggcagacgc
     2521 ccgtggcgcg aacggctcgg cccggggcgc agccttcgct ggtaggtcgg cgtgtgcagg
     2581 ccgcgggggc gccttcgcgg ccgtcgcggg cagatcgggc ggctcgctcg tgaaatcttc
     2641 gacttcgagc gggggctccg actccacttt tttgctcggc gctggcgctg gcggggccgg
     2701 cggcttgccg cggggcggag cgggcggcgg gcccttcctg accgccgggc tcggcagatc
     2761 ggcagcgttg cgatcgaccg gcgacggcag atcttcatcg gcgtgggacg aagccttggg
     2821 ccgaggcggc gctggtggag cagctggccg cggagcttgt ggcgcggccg gagcaactcc
     2881 cagcatcgtg gccttcagcg ccgacgcgcc cttcggtgcg gctgcgggct ctggcggcgg
     2941 caccagggag tcactcgtca caccgaggac tgccccgagc accggaggcg gagctggccg
     3001 ctgcgcttcg ctcggcgatt cgaccttgaa cgagctgccg cacttcgggc agcgcatctt
     3061 gagaccgctc tgcggaatgc ggcgctcatc tacttgatag ggcgccttgc acccggggca
     3121 ttcgaccttg aacatctgcc cgtcgcctca acctttagca ggagagcgcg gcccttgtcc
     3181 cggtttctgc tggacgcccg ccgcactcaa cctcgaaacg gcccttccct gcgacagttg
     3241 ccggcccatc gcgagtggga tagtggcatc tgccgtcacg aggccacacc gggtttggcg
     3301 gatggccgga ctgacatcga gaagaaaatg caacgaacta ccgctatcgc ggtcggacta
     3361 ctcttcgtac tcgggatcgt gctcgtgctc gtcatggggc gaagctcacc acacgctgcg
     3421 accgcaaccc accctgcgcc ttccgcgagc gcgccgccct cggcagctcc ggtggcctcc
     3481 ctggctgcac cgagcgtgcc ttcggccgcg agctccgcag acgccgtcga gctcgacgag
     3541 atcaaacagt ccgaaggctt cgacaccctg cccgacggcc ggaaagtacc tgagttgcca
     3601 gccaacgcgc ccaaggaggt gtccttcggc gtaattcagg tggcgtacca gggagctcag
     3661 tccgcgccga aggacgcgcg cagcaaggcg gacgccaaga ctcgcgcgag cacgctcgcc
     3721 gccgaagcaa agcaggattt cgcggcagcg gtcgccaaag gcgatcgcgg ctcgacgtcc
     3781 gacgccggtc gcgtcccacg tgacgtgctc gaacccgtcg tcgagtattt gctcttcacg
     3841 ttgccgaagg gcgaggtcta cccggaaccg atcgacacgc cgcgcgggta ttgggttatt
     3901 cgccgcaatc gttgatattc tgagcgccgt gaccgcgggc cggcgaagtt cgcgcgtccg
     3961 acatgcgatg ttcgaacaca tgtcgtgcgc atggcgacac cgcgatccaa gtcagtaaga
     4021 gcacgcgaag cgaagacggg ggctaggcac ccgcgactac gtcttctaga ttcgggcgga
     4081 gtttccctgg gatgtccagc acttcgaccc aagcgccgcc tcgttcttcg actcctgacg
     4141 aacgagcgct gacttcgagt atcgagcgtc tgcgtgcaga gcacgacgct aacgacaacg
     4201 gtgcgctgca gtccgtgctc ttgcacgaga tgggcgttct cgcggagcgc tcgggcgacg
     4261 aagccgccgc tgcgcgcgac tacctcgcag ccgtaaatat cgatcccgaa tttcgagagc
     4321 cgctcgaacg cttggtggca atcatcgagc gccgccagtc ctaccgaaac ctcggaaagg
     4381 tgctggagag gctcacacgg gtggccgaca ccgcggacga gcgcgcgcgc tcgctctcgg
     4441 atcaagcggc gttcctcgcc gatcagcaga acgagctcga cgcggcccgg cggtgtctcg
     4501 aagaggcagt cgaac
//
LOCUS       DASTKS010000317         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_943714, whole genome shotgun sequence.
ACCESSION   DASTKS010000317 DASTKS010000000
VERSION     DASTKS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943714"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFQ35_06585"
     CDS             <1..489
                     /locus_tag="VFQ35_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HET9930332.1"
                     /translation="DSQSGGAASTCRSNTDCNAGFCEKTRCGDDVGHCELRDVFCEGQ
                     RAQPVCGCDNVTYLNDCWRRQAGVPLKTFGECRDQMLTCSRSVDCPNGALCVRLFAQG
                     DACLMGGPGKCWSAPESCEAIPNAPNFMECGTPGPCVDMCTAIRSTGHSFTRVPGGCP
                     QP"
     gene            complement(548..847)
                     /locus_tag="VFQ35_06590"
     CDS             complement(548..847)
                     /locus_tag="VFQ35_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930333.1"
                     /translation="MPFRLEAEAPVRQAVPSPSLTPPQPKVSRFSETLAQLGHRVDAG
                     EGWMRRAMSPAAGGLDAAQLIALQAGIYRYTEAVELVGKLVDRASNGVKTLLQPH"
     gene            complement(903..1610)
                     /locus_tag="VFQ35_06595"
     CDS             complement(903..1610)
                     /locus_tag="VFQ35_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9930334.1"
                     /translation="MTEARDDADALRRFEESLGLVDALARQVGRAIGAAVELEELVSF
                     GREGLLEASRRFDPSRGVPFRAYASYRVRGAIFDGVRKLSRLSRRTHERINGLAAAAA
                     VSEGALEDVVGAAPTAGGPAQADAALAEHLAALATAMAMGLVAPTAHGEEGERVPVEQ
                     ADDPESALGRAELLDLVSGAIGELPHEEAELVRRHYLGGERFDHVAAELGLSKSWASR
                     LHTRAMGRLAKRLRNST"
     gene            2096..2698
                     /locus_tag="VFQ35_06600"
     CDS             2096..2698
                     /locus_tag="VFQ35_06600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9930335.1"
                     /translation="MLRYSLLAAAYASMAGLSLAISELWLDRSPWLYPTPWLALPPAV
                     AHAYSLALGLGLGALVVLVTRRLVERVLWAQELAIALRPFARGLTGLGIVVVAIFSSL
                     GEELLFRGLLVPVIGLFLSALLFGGLHQMKGPSRWAWVGWATVVGLLFGVVFVSTGSL
                     LGPIAAHALINGFNLNYLQNHDPAPSRRGLGGLLGHRSRA"
     gene            2886..3089
                     /locus_tag="VFQ35_06605"
                     /pseudo
     CDS             2886..3089
                     /locus_tag="VFQ35_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT          521 a         1082 c         1070 g          609 t
ORIGIN      
        1 gattcacaga gcggaggcgc cgcgtcgacc tgtcgcagca acacggattg caatgccggc
       61 ttttgcgaga agactcgctg cggagacgac gtcggccact gcgaactccg cgacgtgttc
      121 tgcgaaggcc aaagggccca gcccgtgtgc ggctgcgaca acgtcaccta cctgaacgac
      181 tgttggaggc gtcaggcagg agttccgctg aagacgtttg gcgaatgccg agatcaaatg
      241 ttgacgtgca gtcgctccgt ggactgtccc aacggagcac tgtgcgtacg cctgttcgcc
      301 caaggcgatg cgtgtttgat gggcgggccc gggaaatgct ggagcgctcc tgaaagctgc
      361 gaggccatcc cgaacgcacc gaatttcatg gagtgcggca cgccaggtcc ttgtgtcgac
      421 atgtgcacgg cgattcgctc gacaggtcac tcgttcacgc gagttccggg cggctgccca
      481 caaccttgac gcgactgcgc gcgagcttgc gcccgacgcc atcgaagcgg tcgctcgtcg
      541 gcgcgtctca atgtggctgc agcaacgtct ttactccgtt gctggcgcga tcgaccagct
      601 tgccgacgag ctcgacggct tcggtatagc ggtagatgcc ggcttgcagc gcgatgagtt
      661 gcgcggcgtc gaggccgccg gcggccggcg acatcgctcg acgcatccag ccctcgcccg
      721 cgtccacgcg atggcctagc tgcgcgagcg tttcggagaa ccggctgact ttgggttggg
      781 gtggggtgag cgatgggctc ggcaccgcct gccgcaccgg cgcttctgct tcgagacgaa
      841 atggcatgcg gctcagtcgg ccgcccccga accgagttgc gaaaaaagat cgaacccgcg
      901 cctcaggtag agtttcgaag gcgcttggcg agccggccca ttgcgcgcgt gtgcagccgg
      961 ctcgcccacg acttgctcaa cccgagctcc gccgcgacgt gatcgaagcg ctccccaccg
     1021 aggtagtggc gccgcacgag ctccgcctcc tcgtgcggca attcgccgat tgcgccgctc
     1081 accaaatcga gcaactcggc gcgcccgagc gccgattcag gatcgtccgc ctgctcgacg
     1141 gggacgcgct cgccttcttc gccgtgcgcc gtcggtgcga ccaggcccat tgccatggcg
     1201 gtcgcgagag ccgccaagtg ctccgccaac gccgcgtccg cttgggcggg cccaccagcc
     1261 gtcggcgcgg cgccgaccac gtcttccagc gcgccctcgc tcacggcggc ggctgcggcg
     1321 agaccgttga tgcgctcgtg ggttcggcgg gagaggcgcg acagcttgcg cacgccgtcg
     1381 aatatcgccc cccgcaccct gtaactagcg tacgcacgga agggcactcc gcggctcggg
     1441 tcgaaacggc gagacgcctc cagtaagccc tcgcgcccga agctcacgag ctcttctagc
     1501 tccaccgcgg cgccgatggc tcggccaact tgacgcgcaa gagcatccac gagcccgagc
     1561 gactcttcga agcggcgtaa ggcatcggca tcgtcccggg cctcggtcac tggcgcaatc
     1621 tacaacgaag cgcgaccgaa tttcacgcgc ccacgatgga gttaggtcga tcgtccatcg
     1681 cttgaccagc cgcgcctggc gttttccggc ctcgaggaaa cagagccgtt cgcttcgggc
     1741 tacgtcggcg ccaaaggtca atttccgctt tcgctgcgaa agagctcctt tcgctgctca
     1801 gtttggctgg gtttggggtc gcgtccctgc gaaacggcgg gcttgcgccg cggttcgtca
     1861 gcggactcgc cctttccgag ccgatgagcg atgaggctcg agccgcgagc gcgcggtggc
     1921 cgagcctgtg ttcgacgtct gacgatgcgg acggcaggtc cacgagcgcg catttcggta
     1981 gaggcgtcgc gaccatggcg ctgcgaaact ccgaagcgcg aaaagggtgt cgcccgcgcc
     2041 gctccaccgc ccgcaaactt gaggtcgctt cgagctgtgg catagtcggc tttcgatgtt
     2101 gcgatacagc ctgcttgctg ctgcctatgc cagcatggcc gggctgtcgt tggcgatctc
     2161 ggagctctgg ctcgaccgtt cgccgtggct ctatcccacg ccatggctcg cgctgccgcc
     2221 ggccgtcgct catgcctaca gcctcgcgct cgggctgggg ctcggcgcgt tggtcgtgct
     2281 cgtgacgcgg cgcttggtcg aacgcgtgct ctgggcgcaa gagctcgcca tcgcgctgcg
     2341 cccgttcgcg cgcggtttga ccgggctcgg gatcgtagtg gtcgcgatct tcagcagctt
     2401 gggcgaagag ctcctcttcc gagggttact cgttcccgtc atcggcctct tcttgtccgc
     2461 gctgttgttt ggcgggctgc atcagatgaa aggtccgagc aggtgggcgt gggtaggttg
     2521 ggcgactgtg gtgggcctgc tgttcggtgt cgtgttcgtc agcaccggct cgttgctcgg
     2581 gccgatcgcc gcacatgcgc tcatcaacgg gttcaacctg aactacttgc agaaccacga
     2641 tcctgcgccg tcgcgccgtg gtctcggcgg gttgctcggt caccgcagtc gggcatgaag
     2701 ttcgcaccga ttgcacgtcg cgcgacacca tctcgtatta tgatgccact gcagcggctc
     2761 ctcccagcgg cgctgctttg accaccatgt ccgtgctcga ctcgggcttc cccgaagata
     2821 gaccgcgcgt gctcgtcgtc gacgacgaga agttcattcg cgacatcatc gccgactttt
     2881 tgggcatgga gggctacatc gttcggaccg ccgaagatgg aaccagcgcc gtcaacgagc
     2941 tcgagcgagc tcgctacgac atggtcatca gcgatctgaa gatgccgaag atgggcggcc
     3001 tcgatctgct ccgcgaagtg acgcgcgccc atcccgatac gctcaccgtg atcatgaccg
     3061 gcttcggcac ggtcgagacc gccatcgacg cgatgaagcg cggcgcctac gactacatct
     3121 tgaagccctt caaggtggac gaggtcgtac tgtgcgtgat tgagctcggt gagcgccgcc
     3181 tggccgtctt ccgccgtgcg gacgacgtag ccttccatgc cgaggaaatc cgcaaggatg
     3241 tcccggatga acttctcgtc gtccacgatc aggacgcgag gc
//
LOCUS       DASTKS010000318         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_720715, whole genome shotgun sequence.
ACCESSION   DASTKS010000318 DASTKS010000000
VERSION     DASTKS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720715"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VFQ35_06610"
     CDS             complement(<1..1120)
                     /locus_tag="VFQ35_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930336.1"
                     /translation="MAARARLFPPSPKPAGGIVTPFVRAPKVRVAKAAKDPRFQKVVS
                     QLQQSARKVGQHAPPAKKSAEAQAAAKAPPNEKEAGARANQVDDMKDAKAEKPSTGGF
                     LAVLRAEIEKVMPKNLDEADKFMEGNESSQMKGAVGGNVSQQKQQATGPTETATKAPP
                     DTGAVEGKEVSAVPAEPNAVAPPVPGAEAMPAPKPAAEVNQPAKDTAASADQKLKDAE
                     LTQGQLKKANDPRFSAVITAQDKAKETAAAGPAKYRANEQKALTQAKAGALGDTAKGL
                     VALAGTRNATGAKTKTRQQLAKEKDEAERKKVTDTIASIFDKTKTKVEDKLKTLETDV
                     MAVFDAGADAALANMKNSANTEIERYKDDRYSGITGKAR"
     gene            complement(1120..3198)
                     /locus_tag="VFQ35_06615"
     CDS             complement(1120..3198)
                     /locus_tag="VFQ35_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930337.1"
                     /translation="MTNPAFTDVPHSPREHFRLFALAAAARLIDAAGRALGSHEALLE
                     TFPFVVHYLDEMDRLGALPADGTRFAWREAIRSWEASAEVPLPMRDLARAAGLDYPTL
                     ELLVGVGLIEEDPGFGGLFESAQSGTNQARPTLGLCAKWWSDGDGRAGVDDARIVQER
                     IRRLRELALVEVTNTDLPRHQWAVQVPLPVWDALRGEFTSSPPSQLQYVPFASLERST
                     EPLVDETVLETLRRLPSTLRAGQVDAVIVRGPHHNGRLTVLQWLARALGRGVLHIELS
                     GKPEADAARIRCAGALSTLLHALPVLRLEPLPGETLDVPALQGADCPVGVILGKHGGV
                     RGESIHRAVTLTLELPAPALRLQHLSESLGRSPHDCEALSRRLRLTSGNLRRAASLAQ
                     THAALNGREVPAPEDFREATRTLGRQALETLATPLASAGDWADFSAASETLRELRGLE
                     HRCRFREELHEHVGKSLARSVNSGVRALFQGPSGTGKTLAARLLASALDRDLYRVDLA
                     AVVNKYIGETEKNLARIFAAAEELDVVLLFDEGDALLARRTGVSNANDRYANLETNFL
                     LQRLESFEGIAVVTTNAAELIDDAFQRRMDAVIDFRAPEPPERWALWQIHLPEDHRVD
                     TALLREAASRCQLTGGQIRNAVLHASLLALSDGGVLCSEHLDGAIRREYRKSGGVCPL
                     RTPTVYTGRA"
     gene            complement(3202..>3931)
                     /locus_tag="VFQ35_06620"
     CDS             complement(3202..>3931)
                     /locus_tag="VFQ35_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930338.1"
                     /translation="VNVRGVHADWSEPVVTRFGGAFYLVNVALALGLYPDFTRPASLG
                     LWLPLWSYLRQVSARLLAEEPPDAIWSLLSDLTPFGGSLTEHEEPRDWFVEPDWLRGF
                     TPPKEWLATQSSSRLRILHPAGFAAIDARADLDPALIAAKLSEYGTGPLRFAEAEPRT
                     SAQWIDWHVEYLRARLRLALRVDDSQIGPLLLEHRARVHVTATRIDVVFSLEELPIAI
                     RLAGLDRDPGFLPTEARSLHFHFV"
BASE COUNT          630 a         1247 c         1318 g          736 t
ORIGIN      
        1 agcgcgcctt gccggtgatc ccgctgtagc gatcgtcctt gtagcgctcg atctcggtgt
       61 tggcgctgtt cttcatgttc gcgagcgcgg cgtcggcgcc cgcgtcgaag acggccatga
      121 cgtccgtctc gagcgtcttt agcttgtctt cgaccttggt tttggtcttg tcgaagattg
      181 acgcgatcgt gtcagtgacc ttcttgcgct cggcctcgtc cttctctttc gcgagctgct
      241 ggcgagtctt ggttttggct ccggtcgcgt tgcgcgttcc ggcgagagcg accaagcctt
      301 tggcggtgtc gccgagcgcg cccgccttgg cttgcgtgag cgccttttgt tcgttcgcgc
      361 ggtacttcgc tgggccggca gcggcagttt ctttggcctt gtcttgcgcg gtgatgacgg
      421 cgctgaatcg agggtcgttc gctttcttga gttgcccctg cgtgagctcg gcgtccttca
      481 gcttctggtc ggcgcttgcg gccgtatcct tcgcaggctg attgacctcc gccgccggtt
      541 tcggggcggg catcgcctcg gcgcccggaa ccggaggagc gactgcgttc ggttcggcag
      601 gtacggcgga gacctctttg ccttcgacgg ctccggtatc gggaggagcc ttggtcgcgg
      661 tctcggtggg cccggtcgct tgctgtttct gctgcgagac attgccgccc accgcgccct
      721 tcatctgcga ggactcgttg ccctccatga acttgtcggc ttcgtccaag ttcttgggca
      781 tcaccttttc gatttcggcg cgcaggaccg cgaggaagcc tcccgtgctg ggcttctccg
      841 ccttcgcgtc cttcatgtcg tcgacttgat tggcgcgggc gccagcctcc ttctcgttgg
      901 gtggcgcctt cgcggccgcc tgcgcttcgg ctgatttctt ggcgggagga gcgtgctgcc
      961 cgacctttcg agcgctctgc tgaagctggc tgacgacctt ctgaaagcgc gggtccttgg
     1021 cagctttcgc gacacgaact ttgggcgcgc gaacgaaggg cgtgacgatc ccaccggccg
     1081 gctttggcga aggggggaag agccgcgccc gagcggccat taggctctcc cggtgtacac
     1141 cgtgggcgtc cgaagcgggc acactccgcc gctctttcgg tactcgcgcc ggattgcgcc
     1201 gtcgagatgt tcggagcaaa ggactccgcc atccgaaagt gcaagtagcg aagcgtgaag
     1261 gaccgcgttg cggatctgac cgccggtcaa ctggcagcgc gaggcagctt ctctcagcag
     1321 ggcggtatcg acacggtgat cttcaggcag atggatttgc cagagcgccc agcgttccgg
     1381 aggctcgggc gcccggaaat cgatcacggc gtccatgcgg cgctgaaatg cgtcgtcgat
     1441 cagctcggcc gcgttggtgg tgacgacggc gatgccttcg aacgactcca agcgttgcag
     1501 caggaaattc gtctccaggt tcgcatagcg gtcgtttgcg ttactgacgc cggtgcggcg
     1561 cgcgaggagc gcgtctcctt cgtcgaacag cagaacgacg tcgagctcct cggcggcggc
     1621 gaagattcgc gcaaggttct tttcggtctc gccgatatac ttgtttacga ccgccgcgag
     1681 atccacccgg taaagatcgc ggtcgagcgc gctggcgagc agtcgagcgg cgagggtttt
     1741 gccggtgccg ctcggccctt ggaacaatgc gcgtaccccg ctgttcacgg agcgcgccag
     1801 ggatttgccc acgtgttcgt gcagctcctc ccgaaatcga cagcgatgct cgaggccgcg
     1861 cagctcgcgc aacgtttcgc tcgccgcgct gaagtctgcc cagtcaccag cgctcgcgag
     1921 cggagtcgcc agcgtctcga gagcctgcct cccgagcgtg cgcgtcgctt cacggaagtc
     1981 ctctggagcg ggaacctcac gtccgtttag agcagcgtgc gtttgtgcga gtgaggcggc
     2041 tcgacgcagg ttgccgctgg tcaagcgaag tcgccgcgag agggcctcgc aatcgtgcgg
     2101 ggagcgcccg agcgactcgg acaggtgttg caggcgcagg gcgggcgccg gtagctccaa
     2161 agtgagtgtg acggcgcggt ggatggattc tccgcgaacg ccgccatgct tgccgaggat
     2221 gactccaacc gggcaatccg cgccctgaag tgcggggacg tcgagggtct cgccaggcaa
     2281 cggctccaag cgcagcactg gcagcgcgtg aagcagcgtc gacagcgcgc cggcacagcg
     2341 aatgcgggct gcgtccgctt cgggcttgcc cgaaagctcg atgtgcagca cgccgcgtcc
     2401 gagtgcccgc gcgagccact gcaacacggt gagcctgccg ttatggtgcg ggccgcgaac
     2461 gatgaccgcg tcgacttggc cggcgcggag cgtcgacggc aatcgtcgca aagtttcgag
     2521 gaccgtttcg tcgacgagcg gctccgtcga gcgctccaga cttgcgaacg gcacatactg
     2581 aagctgactc ggcggcgagc tcgtgaactc gccccgcaga gcgtcccaaa ctggcaacgg
     2641 cacctgaacc gcccattgat ggcgcggcag gtcggtgttc gtgacctcca cgagcgcgag
     2701 ctcgcgcagc cgccgaatgc gttcctgcac tatgcgcgcg tcgtcgacgc cggcgcgccc
     2761 gtcgccgtca ctccaccact tggcacagag ccccagcgtc ggtcgggctt gattcgtccc
     2821 gctctgggcg ctctcgaaca atcccccaaa accgggatcc tcttcgatga gccccactcc
     2881 gaccagcagc tcgagcgtcg gatagtcgag cccagccgct cgggcgaggt cgcgcatcgg
     2941 taaaggcacc tctgcgctcg cctcccaaga acggatcgcc tcgcgccagg caaaccgagt
     3001 tccgtccgcg ggcaacgctc cgagccgatc catctcgtcg aggtagtgga cgacgaatgg
     3061 aaacgtctcg agtagcgctt cgtgcgaccc gagcgcccgc cccgccgcgt cgatcaagcg
     3121 cgccgctgcg gcgagcgcga acaagcgaaa gtgctcgcgc ggcgagtgcg gaacgtcggt
     3181 gaaggcgggg ttcgtcatga ctcagacaaa gtgaaaatgc agagagcgcg cctcggtcgg
     3241 cagaaaacca gggtcgcgat ccaacccggc gagacggatc gcgatgggaa gctcttcgag
     3301 tgaaaaaacg acgtcgatcc gcgtcgccgt cacgtgcaca cgcgcgcggt gctcgagcaa
     3361 caacggtcca atttgcgaat cgtcgactcg aagcgccagg cgtaagcgtg cgcgaaggta
     3421 ctcgacgtgc caatcgatcc actgcgcgct cgtccgcggc tcggcttcgg caaagcgcag
     3481 cggccctgtt ccatattcgc tgagcttcgc cgctatgaga gccgggtcga ggtctgcgcg
     3541 cgcatcgatc gcagcgaagc ccgctgggtg aaggattcgc aggcgcgagc tactctgcgt
     3601 ggccaaccac tctttgggcg gcgtgaagcc acgcagccag tcgggttcga cgaaccagtc
     3661 tcgcggctct tcgtgttcgg tgagagaccc gccaaaaggc gtgaggtcgg agaggagtga
     3721 ccagattgca tcgggaggct cttcggccaa gagccgcgcg ctcacttggc gcaggtagga
     3781 ccagagaggc agccaaagac caaggcttgc aggtcgcgtg aagtctggat agaggccgag
     3841 ggcgagtgcc acgttgacga gataaaaggc tccgccgaat cgagtgacga ctggctcgga
     3901 ccaatcggcg tgcacgcctc gaacgttgac g
//
LOCUS       DASTKS010000319         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1614402, whole genome shotgun sequence.
ACCESSION   DASTKS010000319 DASTKS010000000
VERSION     DASTKS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614402"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFQ35_06625"
     CDS             complement(<1..263)
                     /locus_tag="VFQ35_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930339.1"
                     /translation="MFARVAFVAYNTYREAVRARILHGLFGLALATSAYTLAVGAFAN
                     RAQLRVVSDLGAASVSIYGIVVAVVLGATSLYRELELKTIFPIL"
     gene            complement(288..929)
                     /locus_tag="VFQ35_06630"
     CDS             complement(288..929)
                     /locus_tag="VFQ35_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930340.1"
                     /translation="MRLSSSRPGLIAVFGFSSFLLLPVNAAAEPRTAALGVSEREALS
                     SGQRIERPLAFETAEGAYVGGVAYQVVQRAPEQILAALLDASNLPAMLPRTQAARVVS
                     VEGGLTRIELTQGAAPFLARYTIVVQPSVDGSELRFWLDQRAPHDVRDVWGFFRVKPF
                     GPGRTLLSVGAAVDLGGGLLRPLLDAKVRSAVLGAVTGIRDFVEPRRLAAVGY"
     gene            complement(1189..>2378)
                     /locus_tag="VFQ35_06635"
     CDS             complement(1189..>2378)
                     /locus_tag="VFQ35_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930341.1"
                     /translation="AELLPNEPVLAPLTGVAHDLTSACSGALGELVYRFELAEPSDVV
                     LSAIPLDTNGAPFLSLRSEACVLNRPSEMDCRVSSLARLYRRALDKGVYYVAVGSTGP
                     SDVQLRLSVSPPSVAPLDEGCSAPPTLPPNSTVDLELNNHVNTYQSACLPGAPDATYA
                     FSIAERSDVLFVERLSSGDTGAISIVSPGCDTRTSRLCVSADARALCACSPFTGSVCS
                     DSAPLRARAYDVPAGEYAVVVESANGSPAQITAFTRPALPSTLVTLSDGCNDAISIPE
                     IGGRFSGNTGNANSDFTASCDFGVGAPLGAPDQLLKMHLSESRRVILDAGGSDYATII
                     AVRTGAECPGREVEKACAPGCSTRRSFLDLTLPAGDYYVQIEGYVGASGRWVLDVFFA
                     DPE"
BASE COUNT          468 a          776 c          781 g          353 t
ORIGIN      
        1 aggatcggga aaatcgtctt gagctcgagc tcccgataga gcgaagttgc cccgagcacc
       61 acggccacga cgattccgta aattgaaacc gacgcggcgc cgagatcact caccacgcgg
      121 agctgagctc gattcgcgaa agctccgacc gcgagcgtgt aagccgaggt ggcgagcgcc
      181 aggccgaaca gcccgtgcag gattcgcgca cgcacggcct ctcgataggt gttgtaggcg
      241 acgaaggcga ctcgagcgaa catccggggc gggctctggg cagacgctca gtagcctacc
      301 gcagccagcc gtcgaggttc gacgaaatct cgaatgccgg ttacggcacc gagaaccgcg
      361 cttcgtacct tggcgtcgag aagcggacgg agcagtcctc cgccgagatc gacggctgcc
      421 ccgacgctta gcagcgtgcg ccccggacca aacggcttca cccggaaaaa gccccacacg
      481 tcgcgcacgt cgtggggcgc gcgctggtcg agccaaaacc gcaactccga cccgtcgacg
      541 ctcggctgga cgacgatggt gtagcgagcg agaaaaggtg ccgctccctg cgtcaactcg
      601 attcgggtga gcccgccctc gacggagacc acgcgcgcag cttgggttct cggcaacatc
      661 gcaggcaggt tgctcgcgtc gagaagggcg gccaggattt gctccggcgc gcgctgcacg
      721 acttgataag cgactccgcc gacgtacgcg ccctcggccg tctcgaaagc gagcggccgc
      781 tcgatgcgct ggcccgaact caaggcctcg cgttcactca cgccgagcgc cgcagtccgc
      841 ggctcggcag ccgcgttgac cggcaataac agaaacgacg aaaacccgaa taccgcgatc
      901 aggcccgggc gcgacgagct caaacgcatg cggccacaga ctaaccacgt tcgctgaacg
      961 ccgccagtga ctggcgtcga tcaagcgcat gacgcacgac atcatgccgc ccccatcgat
     1021 cgtatccaag gtcgacgtat ccgccacgcg agcggcgtat ttccacgagg aaacgagaaa
     1081 acccttcggc gccaatcgct tggcgatcga ccgcagcgaa gcctcatgcg gaaagattgc
     1141 tgccgaacgc gcgtacagcg cacgcgcccc gtccggcact aaccaggctc attctgggtc
     1201 ggcgaaaaag acgtcgagca cccagcggcc cgaggccccc acgtaaccct cgatttgcac
     1261 gtagtaatcg ccggcaggca gcgtcaggtc caggaaactt cgtctggtgc tgcatccggg
     1321 cgcgcaggcc ttctcgactt cgcggcccgg gcactcggcg ccggtgcgaa cggcgatgat
     1381 cgtcgcgtag tcacttccgc cagcgtcgag gatcacgcgg cgagattcgc tgagatgcat
     1441 cttcagtagt tggtcggggg caccgagcgg cgcgcccacg ccgaagtcac aacttgccgt
     1501 aaagtcggag ttcgcgttgc cggtgttccc gctgaagcga ccgccgattt cggggatcga
     1561 gatcgcgtcg ttgcagccgt cgctcagagt gacgagtgtc gaaggcaacg cgggccgagt
     1621 gaacgcggtg atctgagcgg ggcttccgtt cgcgctttcg acgacgaccg cgtattcgcc
     1681 ggcggggacg tcgtaagcgc gggcccgcag cggcgcagag tcggaacaca ccgagccagt
     1741 aaacggcgag caggcacaca atgcgcgcgc atccgcgctg acgcagaggc gactcgtgcg
     1801 cgtgtcacag cccggcgaga cgatgctgat cgctcccgta tcgccggaag acaaccgttc
     1861 cacgaacaat acgtcggagc gctctgcgat tgaaaacgcg taggttgcgt cgggcgcgcc
     1921 gggtagacac gcgctctggt aagtattgac gtgattgttg agctcgaggt cgaccgtact
     1981 gttcggcggc aatgtggggg gcgccgagca gccttcatcg agcggcgcca cgctcggagg
     2041 cgaaacgctg agccgaagct gcacgtcgct cggtccggtc gagccgaccg cgacgtagta
     2101 gacgcccttg tccaacgcgc ggcgatagag gcgcgccagc gagctcactc gacaatccat
     2161 ttcgctcggc ctgttgagga cgcacgcctc ggagcgcaac gagaggaacg gcgcgccgtt
     2221 cgtgtcgagt gggatcgccg agagcacgac gtcggacggc tcggcgagtt cgaatcgata
     2281 cacgagctct ccgagcgcgc cgctgcacgc gctcgtcagg tcgtgcgcga cgccggtgag
     2341 aggagccaac accggctcgt tcggcaggag ttcggccg
//
LOCUS       DASTKS010000320         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1167664, whole genome shotgun sequence.
ACCESSION   DASTKS010000320 DASTKS010000000
VERSION     DASTKS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167664"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFQ35_06640"
     CDS             <1..985
                     /locus_tag="VFQ35_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9930342.1"
                     /translation="TSLVAAATGKLDGMMFVAGALLGVGLFGESLPAFESFFLSGDMG
                     RFTLSDWLGLPIGAVLVLVVVMALTLFVGAEALERRARADVSYARDARTFPLFRRLGA
                     ATLLLAALLVAVRGQPSAEARLAMNPALQRLEAERAMFVDPAEVVALRKDLNLHVDVL
                     DLRSEAEFNLFHLAHAERVSSAALFDREFVRRLQEAEPTTVTFLLGRNEAQALAAWRE
                     LRTRGVLNLYIVEGGVDHWLDLYPVPDCVAERVASSTPGTTTWRFRYANGSRSPSAAP
                     ELAFSRRFAVPCSDVPANASSAPQHEVTWPEHTYTSRVKLQRKTLVKGGCG"
     gene            1195..2325
                     /locus_tag="VFQ35_06645"
     CDS             1195..2325
                     /locus_tag="VFQ35_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930343.1"
                     /translation="MNTSSFFVPFRRSRLAALALVGFSALALPARAQPLDDEISDFAA
                     DVMSDDYAAKRYSAARKKLASALKKCGKKSCSRQVRAELQRDLGLVYFALGKKRLARR
                     SFQKAIELDPNGYLEPELADDEASEAYRALGGHVGDAERVPEIEGEDEEGESAADEAP
                     PPPHDARGPKHGWFSLTMQQDLLLHSETRPVCGSNEYACFSGGNEYAGPIWPAYGNRI
                     EGGLAFATRRVLVGFDWLFGKNFQLGARFGVAFGGGPTAGKTSKFTPAHGELRVAYYF
                     GRNPFASAGVRPYFALGGGMAEVQSKLAVDFYRDAAAYNQMRQDTVDAWRTSGRGFVA
                     PTLGTQFAFSRNTAIALELRMMVMLGTTGLAPAGSVGFVQGL"
     gene            complement(2351..>2544)
                     /locus_tag="VFQ35_06650"
     CDS             complement(2351..>2544)
                     /locus_tag="VFQ35_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HET9930344.1"
                     /translation="QNMFVDYMKEKNITDFTYWSINPESGDTGGVYNHAYTQSNKSGW
                     GVWQGFDTEKVGMLTALVR"
BASE COUNT          439 a          809 c          814 g          482 t
ORIGIN      
        1 gacgtcgctc gtggccgccg caactggaaa gctcgacgga atgatgttcg tcgccggcgc
       61 gttgctcgga gtgggcctct tcggagaaag cctacctgcg ttcgaatcct tcttcctctc
      121 gggcgacatg ggccgtttca cgttgtccga ctggctcggc ttgcccatcg gcgcagtgct
      181 cgtgctggtt gtggtcatgg cgctcacgct cttcgtgggg gcggaggctc tcgagcgacg
      241 ggcgcgcgct gacgtcagct acgcgcgcga tgcgcggacc ttcccactat ttcgccgcct
      301 gggcgcggcg acgctgctgc tcgcggccct gctcgtcgca gtgcgcggtc agccgagtgc
      361 agaggcaaga ctggccatga acccggcgct ccagcggctc gaggccgagc gagcgatgtt
      421 cgtcgatcct gccgaagtgg tcgcgctccg caaagatctc aacttgcacg tcgacgtgct
      481 cgacttgcgc tccgaggccg aattcaattt gttccacctg gcccacgcgg agcgcgtgag
      541 cagcgcagcg ctcttcgacc gtgagttcgt gcgccgactc caagaggcgg agccgacgac
      601 ggtgacgttt ctactcggcc ggaacgaagc gcaggcgctc gcggcgtgga gagagctccg
      661 cacccgggga gtgctgaacc tctacatcgt cgagggcggc gtggatcatt ggctcgatct
      721 gtatcccgta cccgactgcg ttgcggagcg cgtcgcgtcc tccactccag gcaccacgac
      781 ttggcggttc cgctacgcga acgggtcgcg ttcgccgtcc gcagcgccag agctcgcgtt
      841 ttccaggcga tttgccgtcc cgtgcagcga cgttcccgcg aacgcttcga gcgcacccca
      901 gcacgaggtc acctggcccg agcacacgta cacgagccga gtgaagttgc agcgcaagac
      961 actcgtaaag ggcggctgcg gctaaccacg aggccgcgat cgttccaagc ggaccgggac
     1021 cgtcggtcga tcgcgcacct ctcgcacaac gttgctgcgc accgacttcg gtttcgaagt
     1081 gaggccgaga cgaatttgct cgagcccctc gcaacacacg gattgcgagc ggcgctcgtt
     1141 tcgtgtttac gtgcggcctc aactctgcag ccaacccttt ctcgagattg ggccgtgaac
     1201 acgtcttctt tcttcgtacc gtttcgcagg tcgcgcttgg ccgcgctggc actcgtgggg
     1261 ttcagcgccc ttgccctccc ggcgcgagcc cagccgctcg acgatgaaat ttctgatttc
     1321 gctgccgacg taatgtccga cgactacgcc gccaaacggt acagcgcagc ccgaaagaag
     1381 ctcgcgtctg ccctcaagaa atgcggcaaa aagtcgtgct cgcgccaggt tcgagccgag
     1441 ctccagcgcg acttggggct cgtgtacttc gcgctcggaa agaagcggtt ggcgcgccga
     1501 tcgttccaga aggccatcga actcgatccc aatgggtacc tcgagccgga gctagcggac
     1561 gacgaagcga gcgaagctta ccgcgcactc ggcggacacg tgggagacgc cgaacgagtc
     1621 cccgaaatcg aaggcgaaga cgaggaggga gaatcggcgg ctgatgaagc gcctccgccg
     1681 ccgcatgacg ctcggggtcc gaagcacggc tggttttctc tcactatgca gcaagatctg
     1741 ctgctgcatt ccgagacacg accagtatgc gggagcaacg agtacgcctg cttcagcggt
     1801 ggcaacgagt acgcgggtcc gatttggcct gcttatggga accgaatcga aggaggtttg
     1861 gcgttcgcca cgagacgcgt tctcgtcggc ttcgactggc tattcggaaa gaactttcag
     1921 ctcggagcac gctttggtgt cgcgttcggc ggcgggccta cggctggaaa gacgagcaag
     1981 ttcacgcccg cgcacggaga gctccgggta gcctattatt tcggccgaaa cccgttcgcg
     2041 agcgctggcg ttcgtcccta tttcgcgctc ggcggcggga tggcggaagt tcagagcaag
     2101 ctcgccgtcg acttctaccg cgatgccgcc gcgtacaatc aaatgcgaca agacacggtc
     2161 gacgcctggc gcacgagcgg ccgcggcttc gtcgcaccga ccttggggac tcaattcgcg
     2221 ttcagtcgca acacggccat cgctttggag ctgcgcatga tggtgatgtt gggcacgacc
     2281 ggcttggccc ccgcgggaag cgttgggttc gtccaaggac tctgacactg agcgtgcgcg
     2341 cacggcgagc tcagcgcacc aacgccgtca gcatgccgac cttttcggtg tcgaagcctt
     2401 gccagacgcc ccagcccgac ttgttcgatt gggtgtaggc atggttgtaa acgccgcccg
     2461 tgtcgcccga ctcggggttg atggaccagt acgtgaagtc ggtgatgttc ttctccttca
     2521 tgtagtcgac gaacatgttc tgcc
//
LOCUS       DASTKS010000321         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1614501, whole genome shotgun sequence.
ACCESSION   DASTKS010000321 DASTKS010000000
VERSION     DASTKS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614501"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFQ35_06655"
     CDS             <1..426
                     /locus_tag="VFQ35_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930345.1"
                     /translation="LRVEARSLTDGRALLEKVVPLSLEPGERRVALELDISDFDAKST
                     LVYAELFGKGTFRLLAEPKDSELSVPKLDASIEGSELVLRSDLPVVDLYLWDDEGDVA
                     FSENFVTLNSGACRRVRMHGAPVRLRARSLAGEHLLRLA"
     gene            complement(454..1227)
                     /locus_tag="VFQ35_06660"
     CDS             complement(454..1227)
                     /locus_tag="VFQ35_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9930346.1"
                     /translation="MNRPLRIEVAGQTDVGRKRSHNEDNFAIMAEYGLYVVADGMGGH
                     ASGEIASKMAVDTLQEFFASTADDPERTWPYKMDRSRGYEENRLITGIKLCNLRIYEQ
                     AQRNAKQRGMGTTLVALFAVEDGVYIAHVGDSRVYRIRERRIEQLTEDHSLLNDYKKM
                     KRLTDEEIANFPHKNVIVRALGMKDTVKVDTRFEAPRAGDVMLLCSDGLCGPVTDERM
                     LEIVLGSRDLPNATQRLIETANENGGPDNITCVLARWVE"
     gene            complement(1357..2553)
                     /locus_tag="VFQ35_06665"
     CDS             complement(1357..2553)
                     /locus_tag="VFQ35_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9930347.1"
                     /translation="MSVKLWPRLAFAVTALTTTAPVQAQALGAGMLAAPSKPASSAPA
                     TTSVPAPTAPAPAATPAPPAASAPEKAASALDKSGIVLVERAGQVVALGAVLNGDGRV
                     LTSLSRLGTGQLFVRYTNGNAETVRIGHSDASRDLALLVPKTAKNQKGVKAADGPLPQ
                     GSAKLTGFALGPNRNLTPQPQTLQGLSELNGHAVLKLGTVPKPAELGAPLLDERGDAA
                     AIVVSGCAAGTAGCTAPPVALPVSEVRAFLRTRPASAGFQLPRLGIVGRSADNGVVRG
                     LWITSVDPQSPASALGLNANDNPEVADVLVAIGGNPVPTEDALRGILARHAPGDRLDL
                     LVFGKGAYRTLNVRLGPPAAPSPAAPVAPQPPAAPKAKAAPSAPIAPTPPKAPVPPPA
                     PTTPTK"
     gene            complement(2631..3314)
                     /gene="rpiA"
                     /locus_tag="VFQ35_06670"
     CDS             complement(2631..3314)
                     /gene="rpiA"
                     /locus_tag="VFQ35_06670"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET9930348.1"
                     /translation="MDKDRVKRLAAEAALAKLPPEGLIGLGTGTTARHFILAVAELVR
                     AGKKYVGVATSEQSRSIAVAAGLPLRDDAGPWDVAVCVDGADEVSAELDIIKGGGGAH
                     TREKIVNFAARLNVIVVDESKLSQKLGERWAVPVEVLPFGHCETAKLMQAYGKPALRL
                     RDGAPFRTDSGNYVYDVATGPIDEPQALHLALKTIPGVVETGLFCGRADVVLVARESG
                     IEELSRKAR"
     gene            3524..4249
                     /locus_tag="VFQ35_06675"
     CDS             3524..4249
                     /locus_tag="VFQ35_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930349.1"
                     /translation="MSSFIETPKRPATQEVRQAQPVIHVTTVRSSGCLPSLVGSVLVL
                     GGIAFAASQLFGGFSQLTSRVTGATSGKPFNLPFPLPSAISLPQPLAEVDIFKDPSLV
                     KRRFEERLGKPVMVKELVLYPSYAIAEAQDPANKQHLDRYVYRSGAFDKPDPVRLFGG
                     ERDVNKVVFPLDQIDFDKLAGMIQSAPKELPNVEESKVTHVILTRDVFTKGVPAVRVY
                     VSGPRDSGYVEYDAKGKRGRVVH"
     gene            4373..>4681
                     /locus_tag="VFQ35_06680"
     CDS             4373..>4681
                     /locus_tag="VFQ35_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930350.1"
                     /translation="MIRHALFTAAASIAIALPAAAQTAPEASALSLSWVRGEGAEGCI
                     DRRALMHAVRARLGRDPFADEAERGIEGEITRQGEKFVATLRVRAENGLELGQRRLEG
                     P"
BASE COUNT          746 a         1508 c         1565 g          862 t
ORIGIN      
        1 ctgcgcgtcg aggcgcgttc gttgacggac gggcgcgcgc tgctcgaaaa ggtagttccg
       61 ctctcgctcg agccaggaga gcgccgcgtc gcgctcgagc tcgacatttc ggacttcgac
      121 gcaaagagca cgctcgttta cgccgagctc ttcggcaaag gcacgttcag actgctcgcc
      181 gagcccaaag acagcgagct ctccgtgccg aagctcgacg catcgatcga aggtagcgaa
      241 ctcgtgctgc gcagcgacct gcccgtcgtc gatctttacc tttgggacga cgaaggcgac
      301 gtcgcgttct cggaaaactt cgtgacgttg aacagcggcg cttgccgacg cgtgcgcatg
      361 catggcgctc cggtgcgtct gcgcgcacgt agcctcgccg gggagcactt gctgaggctc
      421 gcctgagcca cgcgagccgg accgggcgcg tcgctattcc acccagcgcg cgagcacgca
      481 ggtgatgttg tcgggcccgc cattttcgtt ggcggtctcg atcaagcgtt gcgtcgcgtt
      541 cggtaggtcg cgcgaaccga gcacgatctc gagcatgcgc tcgtcggtga ccggtccaca
      601 caggccgtcg ctacagagca acatcacgtc gcctgcgcgc ggcgcctcga agcgcgtgtc
      661 caccttgacg gtgtccttca tgccgagggc gcgcacgatc acgttcttgt gcggaaagtt
      721 cgcgatctcc tcgtcggtga ggcgcttcat cttcttgtag tcgttgagca agctgtgatc
      781 ttccgtcagc tgctcgatgc gcctctcgcg gatgcgatag acgcgactgt cgcctacgtg
      841 agcgatgtaa acgccatctt cgaccgcgaa cagcgcgacg agcgtagtgc ccatcccccg
      901 ctgcttcgcg ttgcgctgcg cttgctcgta gatgcgcaag ttgcagagct tgatacccgt
      961 aatcagccgg ttttcttcgt agccccgcga gcggtccatt ttgtatggcc aggtccgctc
     1021 cgggtcgtct gctgtcgacg cgaagaattc ctgcagtgtg tcgaccgcca tcttggaagc
     1081 gatctcgcca gaggcgtgac cacccatccc gtcagcgacg acgtagagtc cgtactccgc
     1141 catgatggcg aagttgtcct cgttgtgact gcgcttgcgg cctacatcag tctggccggc
     1201 aacttcgatc ctgagaggcc ggttcatctt cgtgcggcag cgccggatct acatcctcgc
     1261 cgccgcgcat ctggttgtca attctactat aaacagctgg tcggcccgtc ggtcacgtta
     1321 gctcgttcgt cgatgcgagc ggacgcgtat ttgttgctac ttcgttggcg tcgtcggagc
     1381 cggtggcggc actggcgctt tgggcggcgt aggtgcgatc ggggcgctcg gcgcggcttt
     1441 ggccttggga gcagcgggtg gctgaggcgc gacgggcgca gccggggagg gcgcggcggg
     1501 cgggcccaat cgcacgttca gggtgcgata ggcacctttg ccgaacacca acaaatccag
     1561 gcggtcccct ggcgcgtggc gcgcgaggat gccgcgcagc gcgtcctcgg tcggaacggg
     1621 gtttccgccg atggcgacga gcacatcggc gacctccgga ttgtcgttcg cgttgagacc
     1681 gagagcggag gcggggctct gcgggtcgac cgaagtgatc cagaggccgc gcacgacgcc
     1741 gttgtccgcg cttctgccga cgatccccaa gcgcggcaat tggaagcctg ccgacgctgg
     1801 gcgcgtccgc aagaacgcgc gcacttcact cacggggagc gcgaccggcg gcgcggtgca
     1861 accagcggtg cccgcggcgc acccgctcac gacgatggcg gccgcgtcgc cgcgctcgtc
     1921 gaggaggggc gcaccgagct cggccggctt cggcaccgtg ccgagcttca acaccgcgtg
     1981 accgttgagc tcggacaagc cttggagcgt ctgcggttgc ggagtgaggt tgcgatttgg
     2041 acccagcgcg aagccggtga gcttggccga accttgaggt aacggtccat cggcggcttt
     2101 gacgcctttc tggttcttcg ccgtcttcgg caccaacagc gcgagatcgc gggatgcgtc
     2161 gctgtgaccg atgcgaaccg tctcggcgtt gccgttcgtg taacgaacga agagctggcc
     2221 ggtgccaagc cgtgacaacg acgtgagcac gcgcccatcg ccgttgagca cggcgccgag
     2281 cgcgacgacc tgaccggctc gctcgacgag cacgatgccg ctcttgtcca gcgcgctcgc
     2341 ggccttttct ggagcgctag ccgcgggagg cgctggagtc gcagcgggcg cgggcgccgt
     2401 gggggcggga acgctcgtcg tcgcgggcgc ggagctagca ggcttcgatg gcgcggcgag
     2461 catcccggcg cccagcgctt gggcctgaac gggggcagtc gtggtgagcg cggtgacggc
     2521 gaaagcgagt cgtggccaaa gcttcactga catggcgctg agtctacacc gccgcggcgc
     2581 ctgctgccgc gagcgcctcg ccggcgcgaa aatttcgcag gcgccgattg tcagcgcgcc
     2641 ttacgcgaga gctcctcgat gccgctctcg cgcgcgacca acacgacgtc ggcgcgcccg
     2701 cagaaaagcc cagtctccac cacgcccggg attgtcttca gcgctaggtg cagcgcctgg
     2761 ggctcgtcga tgggtccggt cgcgacgtcg tagacgtagt tgccggagtc ggtacggaac
     2821 ggagcgccgt cgcgcaaacg cagcgcaggt ttgccgtaag cttgcatgag cttcgcggtc
     2881 tcgcagtgcc caaacggcag cacttcgacc ggcaccgccc agcgctcgcc gagcttttgg
     2941 ctgagcttgc tttcgtccac cacgatcacg ttcaagcgtg ccgcgaagtt cacgatcttc
     3001 tcgcgggtgt gagcgccgcc gccccccttg atgatatcga gctcggcgct gacctcgtct
     3061 gcgccgtcta cgcacacggc gacgtcccag ggacccgcgt cgtctcgcaa cggcaggccc
     3121 gctgcgacgg cgatggagcg gctttgctcg ctggtcgcca ctccaacgta cttcttgccc
     3181 gcgcgcacga gctcagcgac cgcgagaatg aagtggcgtg cagtcgtacc cgtaccgagc
     3241 ccgatgaggc cttcgggagg aagtttggcg agggccgctt cggccgccaa acgcttcacg
     3301 cggtccttgt ccatggcacg ggcgacttat ccgaagcttc cacgcccggc aacgagatcg
     3361 aggtcgagcg aggtgtttgc cgaaccggac cgcgcttttc ccgcgaaacg gagcgtggca
     3421 aagtgcctcg atggcccgct tagtcgccgc gacctgtccc aagtgcgggg caggggtccg
     3481 actcgacccc aaacagcagt tcgttacttg tacgtattgc ggagtgagct ccttcatcga
     3541 gacgccgaag cgaccggcga ctcaagaggt gcgccaggct cagccagtca ttcacgtgac
     3601 gacggtgcgg agctctgggt gcctgccttc gctggtcggg tcggtcctcg tgttgggggg
     3661 catcgccttt gccgcttctc agctcttcgg tggtttctcg cagttgacga gtcgcgtgac
     3721 cggcgcgacc agcggcaagc cgttcaatct tccgttccct ctcccgagcg cgatttctct
     3781 tccgcaaccg ctggccgagg tggacatctt caaagatccg tctttggtga agcgacgctt
     3841 cgaagagcgc ctcggcaagc cagtgatggt gaaggagctc gtcctctacc cgagctacgc
     3901 gatcgcagag gcgcaggatc ccgcgaacaa gcagcacctc gatcgctatg tttatcgaag
     3961 cggcgcgttc gacaagcccg acccggttcg cttgttcggc ggcgagcgcg acgtcaataa
     4021 ggtcgtgttc ccgctcgatc aaatcgactt cgacaaactc gcgggcatga ttcagagcgc
     4081 tcccaaggag ctgcccaacg tcgaagagag caaggtgact cacgtcatct tgacgcgcga
     4141 cgtattcacg aagggtgttc ccgccgtccg cgtctacgtg agcggtccgc gcgacagcgg
     4201 ctacgtcgag tacgacgcca agggcaagcg cggccgcgtc gtgcattagc tgcgacggct
     4261 ttggcgccgg gttcggcccg agcaatcggt tcgcgatggt gctcgcaccc acgacaggca
     4321 atactctcga cccggagcgc cattggtatc gttcaggccc gcgttctatc ggatgattcg
     4381 gcacgccctg ttcacggcgg cggcatcgat tgcgatcgcg ctgcccgctg cggcgcaaac
     4441 cgcgcccgaa gcttctgcgt tgtcgctctc ttgggtgcgc ggtgaaggcg ccgagggttg
     4501 tatcgaccgg cgcgcgctga tgcacgcggt gcgcgctcgg ctcgggcgtg accctttcgc
     4561 cgacgaagcg gagcgtggca tcgaaggaga gataacgcga caaggtgaga agttcgtcgc
     4621 gacgttgcgc gtccgtgccg aaaatgggct cgagctcggg cagcgccggc tcgaggggcc
     4681 t
//
LOCUS       DASTKS010000322        12741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_499383, whole genome shotgun sequence.
ACCESSION   DASTKS010000322 DASTKS010000000
VERSION     DASTKS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499383"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFQ35_06685"
     CDS             <1..561
                     /locus_tag="VFQ35_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930351.1"
                     /translation="LSEGVRWMSASVCGAPLPQAQGQVLRPARVEVPKLELRPLARVT
                     PLKAPPPPAPATPFERFLDAMVRVALGESATHVAAELPLVLRDGRLRPNLLGAEAQTA
                     LKSRGYLGPEGSAASESFVATQSAWRDVLSGASTNLGACGATTLDEWAASLLAALLGR
                     PASRSEELKKALRREGVAAFGMRAAA"
     gene            complement(590..1315)
                     /locus_tag="VFQ35_06690"
     CDS             complement(590..1315)
                     /locus_tag="VFQ35_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9930352.1"
                     /translation="MTELALKLSDLRVSYGGIAAVKGVSLEVNKGEIVALIGANGAGK
                     TSTLKSIVGLVPAQGGSIELFGKDVRGTPTHRIAAAGVSLVPEGRAVFSTMTVRENLE
                     LGSFLKREKGPQAERFERCIRLFPRLGERLAQEAGTLSGGEQQMLAIARALMADPKLI
                     LLDEPSLGLAPKLVNQIFESIVAIAKSGITILLVEQNTRLSLETAERAYVLVTGNVVL
                     SGRAAELREDERIRAAYLGGDAG"
     gene            complement(1312..2103)
                     /locus_tag="VFQ35_06695"
     CDS             complement(1312..2103)
                     /locus_tag="VFQ35_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9930353.1"
                     /translation="MSLLSLNGIEKVFGGLKALRGVTLEVESGQIFGLIGPNGAGKTT
                     AFNVVTGVYRPDAGSVRFDGEDISGWRPAQVASRGIARTFQNIRVFRSMSVVENVLVA
                     GHHLHQASLFATLVHSRRLLEDERALREKARELLDVMGLGEVANDVAGSLPYGSQRRL
                     EIARALMLKPKLVLLDEPAAGMNSQEAEELKKQIRFLRSELGLTVLLVEHNMSVVMGV
                     CDRIHVVDQGKTIAEGTPEEVRTHPRVLAAYLGQETGSVPPKEDA"
     gene            complement(2100..3116)
                     /locus_tag="VFQ35_06700"
     CDS             complement(2100..3116)
                     /locus_tag="VFQ35_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9930354.1"
                     /translation="MSAVSSPRPNWAKRSLVAAATLVGIYAFGILAESVLIEYVQRIV
                     LLAGINVILAVGLNLINGTTGQFSIGHAGFMAVGAYATAFIGTQLHPVFLGVLGNGVL
                     ADGVTFNVALLAGASLSGLAGLLVGIPSLRLKGDYLAVVTLGFGEIIRILFNNATFLG
                     AATGYFGNDPSGLPSYTNLFWVYSWATLIVVLIANVTFSQTGRSLIAIRADEIAAEAM
                     ATPTTPLKVLAFTLSAATAGIAGGLFAHMQQGIRPEDFRFEKSIDMIVMIIVGGLGSI
                     TGAVVGGIFVAVTLELMRDLSQYRLVIYALLLIIIMLVRPQGLFGTSEAPALLRRFRK
                     RGAA"
     gene            complement(3113..4030)
                     /locus_tag="VFQ35_06705"
     CDS             complement(3113..4030)
                     /locus_tag="VFQ35_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9930355.1"
                     /translation="MTELIQQIINGLSLGSIYALIALGYTMVYGILRLINFAHGEVFM
                     VGAYAGYYLAAGLGIDAYEAAGRPFPLALGLVVLIGAMLAAAALGASIELLAYRPVRN
                     APRLTPLITAIGVSLFLQNAALLIFKPDPRRYPPILREVRFELGGVILTNIKLTIFVV
                     AIALMAALRFFVLRTWTGRAMRAVSLHLDAAKLMGIDVDRTIRVCFAIGSALAAAGGI
                     LFGLDQITITPLMGVLTGLKAFVAAVLGGIGNIPGAVVGGVLIGLAEQLTAGYISPDY
                     RDAITFVILILVLMLKPEGLLGVVKQEKV"
     gene            complement(4118..5290)
                     /locus_tag="VFQ35_06710"
     CDS             complement(4118..5290)
                     /locus_tag="VFQ35_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9930356.1"
                     /translation="MHIVRRRVFSVLAVTALALASCKPRGENAGTSKDASKASSEIIV
                     GHYASLTGSTAHFGQDTDKALRLLVKEVNQAGGVHGKKLKVVTLDTRGDSAEAANAVS
                     RLIDVEKVQAIIGEVASSLSLAGGRVAQRRKIPMISPSSTNPKVTEVGDYIFRVCYLD
                     TFQGKAMALFARNNLKLDKVAILRDVKNDYSIGLADAFKAAFTGAGGTIAAEQSYSQG
                     DTDFSAQITAIKGTAAQAIFVPGYYAEVGAIARTAARLGLKVPLLGGDGWDAPDLVKI
                     GGDALNGSYFSNHFAPDVATPKAQKFVADFKAEYGQEPTGLGALGYDAGGVLVEALRA
                     GGDTGPAALRDRLAAVKDFEGVTGKISIDAGRNAQKGAVVLKIENGKPRYEATVQP"
     gene            5495..6379
                     /locus_tag="VFQ35_06715"
     CDS             5495..6379
                     /locus_tag="VFQ35_06715"
                     /inference="COORDINATES: protein motif:HMM:NF037053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930357.1"
                     /translation="MIVVMEPEASAATIENVIAFLVSAGFDVHRSSGQERTILGVVGE
                     VTGNDVSVVRELEGVAQVVRVSEPFRLASRRFRGRSSTAEGPWGNIGGERPWIALEPV
                     GPLGARRTDDDEPPPSTELPYEVAAGRPFDAAVTRSHRALDSVGSLACLSIHPQTSRQ
                     PGLVHFVERGPSWGATAWIMAAEKELQRGEGAVVLLEAGGEYPNGARTLEIAAIARAK
                     QRTHLPIVVDVPTIAQRAKYCEAVACAAIAAGADGVILRVWAGREGEIPRVPSTLRWS
                     AAVELAEKLRAIGAAVRG"
     gene            6376..7203
                     /locus_tag="VFQ35_06720"
     CDS             6376..7203
                     /locus_tag="VFQ35_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET9930358.1"
                     /translation="MTSVAVFGFGLIGGSLALALRAAQSGYRVTAIDRADVVDDAHTR
                     ECADAYVDVADEARLEKVLGESDVTVLAAPVSVIADRLPWVLERARIVTDCGSTKRHI
                     ARRAQACPGARRFVPGHPMAGLPGGGARNAVATLFKGKNWLLCPADSDSEAVRSVEQL
                     IRDTAANIVHMSVDVHDAAVARTSHLPQLLASALALIAEEHSARPAAGPAFERTTLTA
                     GGPAPIWRDIFATNGDEIARAIAELCGTLQPLAEELSRGERTDLADDLLRRARALRG"
     gene            7344..7655
                     /locus_tag="VFQ35_06725"
     CDS             7344..7655
                     /locus_tag="VFQ35_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930359.1"
                     /translation="MRLRSRAYPWFVALFFGACSLNPTPDIPDAPSGAHGQGGSNSSA
                     AGSPNSAFGGSPINVPNGSGGSANPGTAGNSAGAPDAAGGAPEGGTAGAGGEAGASKL
                     D"
     gene            7798..8535
                     /locus_tag="VFQ35_06730"
     CDS             7798..8535
                     /locus_tag="VFQ35_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930360.1"
                     /translation="MRRLLPLWLVLVAQACTLNPFGDPPSDNGNARSSEGGSNGGPPE
                     PVAGRASSGGSVGLGLGGAMSTSQGGGMVCSGCDGSGGSAVPSSSGGSGTENSAGGST
                     GVGGSAAPTSGGSTSADGGGSPAAGASSSGGSTTTANGGASNGASGSSSSYGGSSSSR
                     GASASFGGSSTAGASSVSGGAAGHAGHAGAAGHAGAAGHAGSAGSAGNSGAAGGGGVA
                     GFAGASGGGGVAGISGAAGRGGWGWVN"
     gene            complement(8548..9069)
                     /locus_tag="VFQ35_06735"
     CDS             complement(8548..9069)
                     /locus_tag="VFQ35_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9930361.1"
                     /translation="MTSPNPERIAPTLPELKNIGLFGALSDDVLQHLTSVLTVEMPEA
                     GQVLFREGDAATAMYVVISGEVEVLKRSRSGVDARVAVLGPSDWFGEMSIVDIQPRSA
                     TVRALAPGRLVRVTPSDLDSLYRHDLRAYAIIILNLARELSRRLRVADGIVADLVANV
                     LESYVKRPRAGGS"
     gene            complement(9176..9613)
                     /locus_tag="VFQ35_06740"
     CDS             complement(9176..9613)
                     /locus_tag="VFQ35_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9930362.1"
                     /translation="MITRPLGKQDYDHIVQIIDRWWGGPTTALAHPIFFYELGSLARV
                     VEHDGILVGFLLGFVAPGPPKTGYVHLVGIHPDYRRRGVGKVLYQTFEEDARAAACTR
                     LKAITTLGNEGSIAFHKALGWAVTFVEDYAGPARPRVVFNKNL"
     gene            9875..10540
                     /gene="rpe"
                     /locus_tag="VFQ35_06745"
     CDS             9875..10540
                     /gene="rpe"
                     /locus_tag="VFQ35_06745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9930363.1"
                     /translation="MSIRIAPSLLSADFGRLADEVRVVDKGGADWIHVDVMDGQFVPN
                     ISAGPLFVKAARSATKLTVDAHLMIRDPDRYLERFAEAGADVITVHVEACTHLQRTLA
                     AIRALGKRAGVALNPHTPEEMLRYVLGDVDVILVMSVNPGFSGQGFLSQVLPKIRTLR
                     EWIDRDNLPIDLQSDGGVSPDTARQVVQAGARVLVAGSAVFDQVDRGAAIRAIRAATA
                     DHE"
     gene            10756..11958
                     /locus_tag="VFQ35_06750"
     CDS             10756..11958
                     /locus_tag="VFQ35_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930364.1"
                     /translation="MRGLAAKKPVPGVRLDRAALHAEVARLLADESPTDAIAGNTDLL
                     FALDTVPASFDLQATLLRLYASELAGFYDAKLSRMVLATDLGEQAERMTLYHELVHAL
                     QDQHFDLSHAFDWQPEATDAQAALHALAEGDATAAMTEVFAAALGVPAQRIPPGALKL
                     DATLMQASPNLRDVPGVIARSMIAPYADGLAFVTYLREHSGGWGGVDAAFRRTPVSTE
                     QLLHPDKYVAGEPVAQVAAPEPPSGFEANAFRDVMGEQGLRMLFEDWLPAPAAAKAAS
                     DWGADRLAVFSAGAQRVVRWHLVFDNDAAARRALEAFARGAVRPELGAAYEPLRAFAE
                     GKVARSAIRADQVCQARTQRGAFAASRHGRHVGVWLGPFQRTAAGVLGTSDCTAALAG
                     ARATASAH"
     gene            12282..12695
                     /locus_tag="VFQ35_06755"
     CDS             12282..12695
                     /locus_tag="VFQ35_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930365.1"
                     /translation="MALVAATTIGLSAVQRVRAEVSVGPRDSLPKVSGEYRLIVQSYS
                     ASNVVDGRPLAHARPLASAQRAVTPEELAHGVAVDVVDVGEPANDSAVIVAWLERGSA
                     DLDFDGRRARPSRDAYIGVAAAHSEEARVVLSKSA"
BASE COUNT         2109 a         4151 c         4326 g         2155 t
ORIGIN      
        1 ctctccgaag gggtccggtg gatgtccgcg tccgtctgcg gtgcgccact gcctcaagcg
       61 caagggcagg tgctccgtcc ggcccgtgtc gaagttccga agctcgagct tcggccgctc
      121 gcgcgggtga cgccgctgaa agcaccgccg cccccagccc cggcgacgcc gttcgagcgc
      181 tttttggacg cgatggttcg cgtcgcgttg ggcgagtcgg cgactcacgt ggccgcggag
      241 ctgccgctcg ttttgcggga tggtcggctg cgcccgaacc tgctcggcgc cgaggcacag
      301 acggcgctga agagccgggg ctacctggga ccggaaggca gcgcggcctc cgaaagtttc
      361 gtcgcgactc aatcggcctg gcgtgacgtg ctctccggtg cgtcgacgaa cctcggcgcg
      421 tgcggtgcga ccacgctcga cgagtgggcc gcgtcgttgc tggcggcgct gctcggaagg
      481 cccgcatcgc gctcggagga gctgaaaaaa gccctgcgcc gcgaaggggt ggcggcgttc
      541 ggaatgcgcg ccgcggcgtg atcgcgagcg cgctccgcgc cgtggtgctt cagccggcgt
      601 cgccgcccaa gtaagcggcg cggatccgtt cgtcttcacg gagctcggcg gcgcgtcccg
      661 agagcacgac gttgccggtg acgaggacgt acgcgcgctc cgcggtttcg agcgagagcc
      721 gtgtgttctg ctccacgagc aagatggtga tgccgctctt cgcgatcgcg acgatgcttt
      781 cgaagatttg gttcacgagc ttcggtgcga ggccgaggct cggctcgtcc agcaagatga
      841 gcttcgggtc cgccatcagc gcgcgcgcga tcgcgagcat ctgctgctcc ccgcccgaaa
      901 gagtgcccgc ctcctgtgcg agccgctcgc cgagccgcgg aaacagccga atacagcgct
      961 cgaagcgctc cgcttggggc cccttctcgc gttttagaaa gctgccgagc tcgaggttct
     1021 cgcgcaccgt catggtgctg aacacggcgc gcccttcggg aaccaacgac actcccgcgg
     1081 cggcgatccg gtgagtcggg gtgccgcgca cgtccttgcc gaacagctcg atgctcccgc
     1141 cttgcgcggg cacgagcccg acgatcgact ttagcgtgct cgtcttgccg gcgccgttcg
     1201 cgccgatgag cgcgacgatc tcgcctttgt tcacctcgag cgatacgccc ttcacggccg
     1261 cgattccgcc gtaagaaacg cgcaaatcgc tgagcttcag cgcaagctcg gtcatgcgtc
     1321 ttccttggga ggcacgctgc ccgtctcttg accgaggtac gcggcgagca cgcgcgggtg
     1381 ggtacgcacc tcttcgggag ttccctccgc gatcgttttg ccctgatcga cgacgtggat
     1441 gcgatcgcac acgcccatca ccacggacat gttgtgctcg acgagcagca ccgtgagccc
     1501 gagctcggag cgcaagaagc ggatttgctt cttcagctcc tcggcttctt ggctgttcat
     1561 gcccgcggcg ggttcgtcga gcagcacgag tttcggcttc aacatcagcg cgcgcgcgat
     1621 ttcgaggcgc cgttgcgagc cgtacggcag gctgccggcg acgtcgttcg cgacttcacc
     1681 gagccccatc acgtcgagca gctcgcgcgc cttctcgcgc agggcgcgct cgtcttcgag
     1741 caagcgacga ctatggacca aggtcgcgaa cagcgaagcc tggtgcaggt ggtggcccgc
     1801 gacgagcacg ttctccacca ccgacatcga gcggaacacg cggatgttct gaaaggtgcg
     1861 ggcgatgccg cgcgatgcga cctgcgcggg gcgccagccg gagatgtcct cgccgtcgaa
     1921 gcgaacgctg cccgcgtcgg ggcggtacac gccagtgacg acgttgaatg cggtcgtctt
     1981 gccggcgccg ttcgggccaa ttagcccgaa aatctggccc gactcgacct ccagggtcac
     2041 tccgcgcagg gccttgaggc cgccgaacac cttctcgatt ccgttcagcg agagcaggct
     2101 cacgcggcgc ctcgcttacg gaaacggcgc agcagcgccg gcgcctccga cgtgccgaac
     2161 aagccttgcg ggcgcaccag catgatgatg atgagcaaca gcgcgtagat cacgaggcgg
     2221 tactgtgaga ggtcgcgcat cagctcgagg gtgacggcga cgaagatgcc gccgaccacc
     2281 gcgcccgtga tcgagccgag cccgccgacg atgatcatca cgatcatgtc gatagacttt
     2341 tcgaagcgga agtcttcggg gcgaatgcct tgctgcatgt gggcgaacag gccccccgcg
     2401 atgccggccg tcgccgcgct cagcgtaaaa gccagcactt tcagcggcgt cgtcggggtc
     2461 gccatcgctt cggccgcgat ctcgtctgcg cgaatcgcga tgagcgagcg acccgtctgc
     2521 gagaacgtca cgttcgcgat caagacgacg atcagcgtgg cccacgaata gacccagaac
     2581 aggttcgtgt agctcggcag gcccgaagga tcgttgccga agtagccagt cgccgcgccg
     2641 aggaacgtcg cgttgttgaa gaggatgcga atgatctcac cgaagcccag cgtgaccacg
     2701 gcgaggtagt cgcccttcaa gcgcaggctc gggatcccca ccaagagccc cgcgaggccg
     2761 gagagcgacg cccccgcgag cagcgcgacg ttgaacgtga ccccgtccgc gagcacgccg
     2821 ttccccagaa cccccaaaaa caccgggtga agctgggtgc cgatgaacgc ggtggcatac
     2881 gccccgaccg ccatgaagcc ggcgtggccg atcgagaact gccccgtggt gccgttgatc
     2941 aagttcagcc caaccgcgag gatcacgttg atcccagcga gcagtacgat gcgctgcacg
     3001 tactcgatga gcacgctctc ggcgagtatg ccgaaagcgt aaatgccgac gagcgtcgcg
     3061 gcagccacga gcgagcgctt ggcccagttc ggtcgcggcg agctcaccgc gctcatacct
     3121 tctcctgctt cacgacgccg agcagcccct cgggcttgag catcaacacc aagatcagga
     3181 tcacgaaggt gatcgcgtcg cgatagtcgg gcgagatgta gccggccgtg agctgctcgg
     3241 cgaggccgat caagacgccg ccgacgaccg cacccgggat gttgccgatc ccacccaaaa
     3301 ccgcggccac gaaggctttc agccccgtga gtacgcccat gagcggggtg atggtgatct
     3361 gatcgaggcc gaacagaatg cctcccgcgg cggcaagagc ggaaccaatc gcgaagcaaa
     3421 cgcggatcgt ccggtcgacg tcgatgccca tcagcttcgc ggcgtcgagg tgaagcgaca
     3481 ccgcgcgcat cgcgcgaccg gtccaggtgc gcaggacgaa gaagcgcagc gctgccatca
     3541 aggcaatcgc gacgacgaag atggtgagct tgatgttggt gaggatcacg ccgccgagct
     3601 cgaagcgcac ctcgcgcaag atcggcgggt aacgacgcgg atctggcttg aaaatcagga
     3661 gcgccgcgtt ttgcaaaaac aagctcaccc cgatggcggt gatgagcggc gtgagccggg
     3721 gtgcattgcg aacggggcgg taggcgagga gctcgatgga cgcgccgagc gcggccgcgg
     3781 cgagcatcgc gccgatgagg accacgagtc cgagcgcgag cgggaacggt cgacccgcgg
     3841 cttcgtaagc atcgatgccg agccccgccg ccaagtagta accggcgtaa gcgccgacca
     3901 tgaacacttc gccgtgcgcg aagttgatca ggcgcaagat gccgtagacc atcgtgtaac
     3961 cgagcgcgat gagcgcatag atcgatccca acgaaagacc gttgatgatc tgctggatga
     4021 gttcggtcac gacgtctccg gactcataca ccgaaacgca ggattcttgg ccggtttctc
     4081 ggcgggccag cgcacccgcg cgcgggtgac cgactcctca gggctgaacg gtggcctcgt
     4141 atctcggctt cccattttcg atcttcaaaa ccacggcacc cttctgcgcg tttcgacctg
     4201 cgtcgatgga gatctttccc gtcacaccct cgaaatcctt tacggccgcg agccgatctc
     4261 gcaaggcagc cggtcccgtg tcgcctcccg cgcggagcgc ctcgaccaga acgccgcccg
     4321 catcgtaacc gagcgcgccg agcccagtcg gctcttggcc gtactctgcc ttgaagtcgg
     4381 cgacgaactt ctgcgccttc ggggtcgcca cgtcgggtgc gaagtggttc gaaaaatacg
     4441 agccgttgag cgcgtcgccg ccgattttca cgagatctgg agcgtcccaa ccgtcgccgc
     4501 cgagcaaggg gaccttcagt ccgaggcgcg cagcggtacg cgcgatcgcg cccacctcgg
     4561 cataatagcc gggcacgaag atggcttgcg cggcggtgcc cttgatggcc gtgatttgcg
     4621 cgctgaagtc ggtgtcgcct tgcgaatagc tttgctcggc ggcgatggtg ccacccgccc
     4681 cggtgaacgc ggccttgaaa gcgtcggcga gcccgatcga gtagtcgttc ttcacgtcac
     4741 gcaagatggc gaccttgtcg agcttcaggt tgttgcgcgc gaagagcgcc atcgccttgc
     4801 cctggaaggt gtccaggtag cagacgcgga agatgtaatc gccgacctcg gtgaccttcg
     4861 gattggtgga tgaaggcgaa atcatcggga ttttgcggcg ctgcgcgacg cgaccgcccg
     4921 cgagcgagag gctcgaagcg acttcgccaa tgatggcttg aactttctcg acgtcgatga
     4981 gccgtgagac cgcattcgcc gcttcggccg agtcgccgcg cgtgtcgagc gtgacgacct
     5041 tgagcttctt tccgtgcacg ccaccggctt ggttcacctc tttcacgagt aggcgcagcg
     5101 ccttgtcggt gtcttgaccg aagtgcgccg tactccccgt gagcgaggcg tagtgaccca
     5161 cgatgatctc ggagctcgcc ttcgaggcat ccttggacgt gcccgcgttt tcgccgcgcg
     5221 gtttgcacga agcgagggcg agggccgtga cggcgagcac gctgaagacg cggcggcgaa
     5281 cgatgtgcat ggatggtccg atggggtgac ggccggccgc cccgattggg gccggcaccg
     5341 gctcgaccct atggaatccg agcgtcgcgg gtcaagcatg gccgcgcggt gtctttgctc
     5401 ccgacagatt ctcgcgcggg aaattgcttc gaaacaactc cgaaggcgga agtggcgccc
     5461 gctctcggcg gcttttgcta ggcttgcgag tccaatgatc gtcgtgatgg agccggaggc
     5521 ttcagccgcc accatagaaa atgtgatcgc gtttttggtc tccgcggggt tcgacgtcca
     5581 ccgctcgagc ggccaagagc gcacgattct cggcgtggtc ggcgaggtca ccgggaacga
     5641 cgtttcggtc gtgcgtgagc tcgaaggcgt cgctcaggtc gttcgcgtga gcgagccgtt
     5701 ccggctggca tcacggcgct ttcgcggtcg ctcgagcacc gccgaaggtc catggggcaa
     5761 catcggcggc gagcgaccgt ggatcgcgct cgaacccgtc gggccactcg gcgcgcgccg
     5821 cactgacgac gacgagcccc cgccctcgac ggagctgcct tacgaggtcg ccgctgggcg
     5881 gcccttcgac gcagcggtca cgcgctctca tcgcgcgctc gacagcgttg gctcactcgc
     5941 gtgcttgtcg attcatccgc agacgtcgcg gcaaccgggt ctcgttcact tcgtcgagcg
     6001 cgggccgagc tggggagcca cggcttggat catggcggcc gagaaggagc ttcagcgcgg
     6061 tgagggagcg gtggtgctgc tcgaggccgg tggcgagtat ccgaacggcg ctcgcacgct
     6121 cgaaatcgcc gctatcgcgc gggccaagca gcgcacccat ttgccgatcg tcgtcgacgt
     6181 tcctaccatc gcgcagcgcg cgaaatattg cgaagcggtg gcctgcgcgg cgatcgccgc
     6241 cggtgcggac ggcgtcattc tgcgtgtctg ggcggggcgc gaaggtgaaa tcccgcgagt
     6301 gccgtcgacg ctgcgttgga gcgcggcggt cgagctcgcc gaaaagctcc gcgcgatcgg
     6361 cgcggccgtt cgcggatgac gagcgtcgct gtctttggct tcggcttgat tggcggctcg
     6421 ctcgccctgg ccttgcgagc agcgcagagc ggctaccgcg tgaccgcgat cgatcgcgcc
     6481 gacgtcgtcg acgacgcgca cacgcgcgag tgcgccgacg cttacgtcga cgtcgccgat
     6541 gaagcacggc tcgagaaggt gctgggcgag agcgacgtca ccgtgctcgc ggcgcccgtg
     6601 tcggtgatcg ccgaccggtt gccttgggtg ctggaaagag cccgcatcgt caccgattgc
     6661 gggtccacga agcgccacat tgcgcgtcgt gcgcaggcgt gtccgggtgc gcgtcgcttc
     6721 gttccggggc acccgatggc gggtcttccc ggcggcggtg cccgcaacgc ggtcgcgacg
     6781 ctgttcaagg gaaagaactg gctgctctgt cccgccgact ccgactctga ggcggttcga
     6841 agcgtcgagc agttgattcg agacaccgcc gccaacatcg ttcacatgag cgtcgacgtt
     6901 cacgacgccg ccgtcgcgcg gacgagccac ctgccgcagc tgctcgcaag cgcgctcgcg
     6961 ctcatcgctg aagagcactc ggcgcggccc gcggctggcc ccgctttcga gcggacgacg
     7021 ctcaccgcag ggggccccgc tcccatttgg cgtgacatct tcgcaaccaa cggtgatgag
     7081 atcgcgcgcg ccatcgctga gctttgcggg acgctgcagc cgttggccga ggagctctcc
     7141 cgcggcgagc gaaccgatct cgccgacgat ctgctgcgcc gcgccagagc gcttcgcggc
     7201 taggcgtccc gcagggagtg tgagccagcg cctccgaacg ggctttgggt gccctgccgc
     7261 cgatggagcg agcgaggcaa agtaagtttg gggccaagcg ccgcggctgc ctgttagctc
     7321 gggttttgca gtaccctcca ttcgtgaggt tgcgaagccg tgcctatccc tggttcgttg
     7381 cgctgttctt cggcgcctgc tcgctcaacc ccacacccga cattccggat gctccgagcg
     7441 gcgctcacgg tcagggaggc agcaattcgt cggcggcggg cagtccgaac tctgcttttg
     7501 gcggcagccc aatcaacgtg ccgaacggtt cgggcgggtc cgccaatcct ggaactgcgg
     7561 gcaactccgc tggcgccccc gacgccgcgg gtggcgctcc ggaagggggc acggcaggtg
     7621 cagggggaga agccggagcc tctaagttag actgagtttg cgagggttgt gccagcaccg
     7681 gtcggtcata tcgatccggg tttgtgccaa ggtgtgtcag cggtcgagcc ggacgccgtc
     7741 gaaccagact gaaatcattg tgctctcaga tggtccgagg catgcatcat gcccctcatg
     7801 cgtcgcttgc taccactctg gctcgtcctg gtggcacagg catgcacgct gaacccgttt
     7861 ggcgatccac cctcggacaa tggaaacgcg aggtcttcgg aaggcgggtc gaacgggggt
     7921 ccgccggagc cggtggcggg acgtgcatcg agcggcggct ccgtcggtct cggtctcggc
     7981 ggagcaatga gtacgagcca gggcggaggc atggtctgct ccgggtgcga tggctcgggc
     8041 ggaagcgccg tgccgtcgtc gagcggcggc tcgggcaccg aaaattcagc gggtggaagc
     8101 actggggtcg gcgggagcgc ggctccaacc tctggcggct cgacgagcgc agatggcggt
     8161 ggctctcctg cggccggcgc ctcttcgtca ggcggctcca cgactaccgc gaacggtggt
     8221 gcgagcaacg gggcgtcggg cagcagctcg agttacggtg ggtcgtcgag ctctcgcggc
     8281 gcatcggcga gcttcggcgg ttcgtccacg gcgggtgcct cgagcgtcag cggcggcgcc
     8341 gcgggccatg cgggacatgc cggagccgcg ggacatgccg gagccgcggg acatgccggc
     8401 tcggctggct cagccgggaa ttcgggtgcc gcgggcggag gcggtgtcgc gggatttgca
     8461 ggagcgagtg gcggcggcgg agttgccggc atttccggcg cggctggccg cggcggatgg
     8521 ggctgggtga actgacgtga gccggcgtta gctgccgccg gcgcgcggtc gcttcacgta
     8581 gctctcgagg acgtttgcga ccaaatcagc cacgatgccg tcggcgacgc gcagccggcg
     8641 cgagagctcg cgcgccaagt tgagaatgat gatcgcgtaa gcgcgcaggt cgtgccgata
     8701 cagcgagtcg agatccgaag gcgtgacgcg cacgagcctt ccgggcgcca acgcgcgcac
     8761 ggttgccgag cgcggctgaa tatcaacgat gctcatttcg ccgaaccaat cgctcgggcc
     8821 gagcacggcc acccgcgcgt cgacgccgct gcgactgcgc ttcagcactt cgacctcgcc
     8881 gctgatcacg acgtacatcg cggtcgcggc gtcaccttcg cggaagagca cctgcccggc
     8941 ctcgggcatc tcgaccgtca aaaccgacgt gaggtgctgc aagacgtcgt cactgagcgc
     9001 accgaagagc ccaatgttct tcaactcggg caaggtcggc gctatccgtt cggggttcgg
     9061 ggaagtcatg ctccgctccg ttcgcgcacg gggatgcccc ggtatcgact ggtgcgcaac
     9121 ccaaaaatgc gtccgagttg gacgcgaaac tagtgcaagg tcgtgacgac gctcgtcaca
     9181 agttcttgtt gaacacgact cgcggcctcg cgggccccgc gtaatcttcg acgaaggtca
     9241 ctgcccagcc gagcgccttg tggaacgcaa tcgagccttc gtttccgagc gtcgtgattg
     9301 ccttcaagcg agtgcaggcg gctgcgcgcg cgtcttcctc gaaggtttga tagaggacct
     9361 taccgacgcc acggcgccga taatccgggt gaattccgac gaggtgaacg tagccagtct
     9421 tgggcgggcc cggcgccacg aaccccagca gaaatcccac caggattccg tcgtgttcga
     9481 ccacgcgcgc gagcgacccg agctcgtaga aaaagatcgg gtgggcgagc gccgttgttg
     9541 ggcctcccca ccaacggtcg atgatctgaa cgatgtgatc gtagtcttgc ttaccgagcg
     9601 ggcgggtgat catgtccgtc tctcaacacg acgctcaacg tcgttcgcag cgtaacatgt
     9661 ccccgggata aggctcggcc gcaaatggca aatcgtcgcg agcgagcggc tcggcggctg
     9721 catggatcac acatggtcgc gctactcaca tgttgcgcgc gtgtgctctg cgtactgcga
     9781 agcgacgctt cggctggcgg gccccgactt ttctcgggct tcttcgcgcc cacttcggac
     9841 gtccacgttc gtgagccggt ggtaagacgt gcgcgtgtcg atacgtatcg ccccatcact
     9901 gctgagcgcg gacttcggac gactggccga cgaggttcgc gtcgtagata aaggcggcgc
     9961 cgactggatt cacgtcgacg tcatggatgg gcaattcgtc ccgaacatca gcgccgggcc
    10021 gctgttcgta aaggccgcgc gctccgcgac gaagctcacc gtcgatgcac atttgatgat
    10081 ccgcgacccg gatcgctacc tcgagcgctt cgcggaagcc ggcgccgacg tgatcaccgt
    10141 tcacgtcgaa gcgtgtacgc atctgcagcg cacgctcgcc gcgattcgcg ctctcggtaa
    10201 gcgcgcgggc gtcgccttga acccgcacac ccccgaagag atgttgcgat acgtcctcgg
    10261 cgacgtcgac gtcatcctcg tcatgagcgt caatccgggc ttctccgggc aaggcttctt
    10321 atcgcaggta ttgccgaaga ttcgcacgct gcgcgaatgg atcgaccgcg acaacttgcc
    10381 catcgatttg cagagcgacg gcggggtgag cccggacacg gcgcggcagg tcgtgcaagc
    10441 gggcgcgagg gttctcgtcg cggggagcgc agtgttcgat caagtcgatc gaggggccgc
    10501 gattcgagcg attcgagccg cgaccgcgga tcacgaatga cgcggccggc ggcgctcgcg
    10561 ctcgtgctct gtgcatgcag cggtacgcgg gaaactcctg cccatccgac ccagtcacct
    10621 cccgcggcga acgctcgaac gtcggccggg gcgagcgtcg ctccggtctc gagccgggac
    10681 ccgtcgagtt ccccgccgaa accagatgtc gaagcggacg ctgccgtcgc tagtgcactt
    10741 tcgctcgttt ctcaggtgcg tggtctcgcg gcaaagaagc cagtgccggg cgtgcgactc
    10801 gatcgggcgg cgctacacgc agaggtggcg cgactgttgg cggacgaatc cccaaccgac
    10861 gcgatcgcag ggaacacgga cctcctcttc gcgctcgaca cggtgccggc cagcttcgac
    10921 ctgcaggcga ctctgctgcg cctctacgct tctgaactcg cgggatttta cgacgccaag
    10981 ctgagccgaa tggtcctcgc gacagacctc ggcgagcaag cggagcgcat gacgctctac
    11041 cacgagctcg ttcacgcgct ccaagaccag cacttcgact tgagccacgc cttcgactgg
    11101 caaccagagg cgactgacgc gcaggccgcg ctgcacgccc tcgccgaggg cgacgcaacc
    11161 gcggccatga cggaggtgtt cgcggccgcc ctcggtgtgc ccgcgcaacg aatcccgccc
    11221 ggggccttga agctcgacgc gacgctgatg caggcgtcgc ccaacttgcg ggacgttccg
    11281 ggcgtcatcg ctcgctcgat gatcgcgccg tacgcggacg gcctcgcctt cgttacctat
    11341 ctgcgtgagc actccggggg atggggcggt gtggacgcgg cgttcaggcg gacacctgtc
    11401 tcgaccgaac agctgttaca cccggacaag tacgtcgcgg gtgagccggt cgctcaggta
    11461 gcggcccccg agccgccttc ggggttcgaa gcgaacgcct tccgcgacgt gatgggcgaa
    11521 caggggctcc gaatgctctt cgaagactgg ctaccggctc ctgccgccgc gaaggcagcg
    11581 agcgactggg gtgcggatcg ccttgccgtc ttctctgcgg gggcccagcg cgtcgtgcgt
    11641 tggcacctcg ttttcgataa cgacgccgcc gctcgaaggg cgctcgaagc ctttgcgcgc
    11701 ggcgcggtta ggcccgagct cggcgccgct tacgagccgc ttcgggcttt cgcagaaggc
    11761 aaagtcgcac ggagtgccat tcgtgcagac caggtgtgcc aagcgcgcac gcagcgtggc
    11821 gccttcgcgg catcgcgaca cggccgccat gtaggtgttt ggcttgggcc tttccagcga
    11881 accgcagcag gggttcttgg aacctcggac tgcaccgcgg ccctcgctgg agctcgcgct
    11941 accgcttccg cgcattgacc caggcggcgg cgggctcgcc tcgagctttg ggcaacgatt
    12001 tcagaaactt gacgcgagct cagggtgaga ccgaaagggg gtttcagccg actcgggcgg
    12061 gtgaatggag acccttccgc agcgcgcgaa actgctgagc aaaatcatgc gttgcttctg
    12121 gcacgcagct tgcttatgaa gggttcgtgt tcgcgacgga gaaaccgaag ccctccgcgc
    12181 gcgttttaga gaagcagatg gccgcgcacc ccctcccact tccccgttcc acccgacccg
    12241 ctgggtcgag tcgtggcgcg tcggcgggag ctgggtcgtg gatggctctg gttgcggcga
    12301 cgaccatcgg tctcagcgcg gtgcagcgcg tacgcgccga ggtcagcgtt ggtcctcgcg
    12361 attcgcttcc gaaggtctcg ggcgagtacc ggctcatcgt gcagtcgtac tccgcgagca
    12421 acgtggtcga cggccgtccg ctcgcgcacg cccgtccgtt ggcgtctgcg cagcgagcag
    12481 tcactccgga agagctcgcg cacggcgtcg cggtcgacgt cgtggacgtg ggcgagccgg
    12541 ccaacgactc cgccgtgatc gtggcgtggc tcgagcgagg cagcgccgac ctcgatttcg
    12601 atgggcgacg agctcggccc agtcgtgacg cctacatcgg cgtcgccgca gcgcacagcg
    12661 aagaagcgcg cgtcgtcctg agcaagtcgg cctagggggt gtcccaaggt ccccgccctg
    12721 ctgcgagcca agatccaccg g
//
LOCUS       DASTKS010000323         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1391660, whole genome shotgun sequence.
ACCESSION   DASTKS010000323 DASTKS010000000
VERSION     DASTKS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391660"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFQ35_06760"
     CDS             <1..225
                     /locus_tag="VFQ35_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HET9930366.1"
                     /translation="SFPMGAIVNRSLTLRSGQCHVHRYLKPLLEHIQRGDIDPSFVIT
                     HRLSLDEADKGFDLFLRNRDECEKVVLKAA"
     gene            complement(313..1107)
                     /locus_tag="VFQ35_06765"
     CDS             complement(313..1107)
                     /locus_tag="VFQ35_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9930367.1"
                     /translation="MMNTQTRPLAVVTGASTGIGYELAKCCAEGGFDLLIVADEPEIH
                     SAADELRRAGVAVESLEADLATTEGVDRLYAAARGRPIDALLANAGRGLGRAFLDQDW
                     NDVRRVIDTNVTGTVYLIQKVGREMRNRDAGRILITGSIAGFMPGTFQAVYNGTKAFL
                     DSFAYALRAELKGSAVTVTCLMPGATETEFFDRADMLDTKVAADKKQPASEVAKQGFD
                     AMMRGEGDVVTGWQNKLRVAMSHFLPKEALAEQHRGMAEPGSAYKH"
     gene            1284..>2477
                     /locus_tag="VFQ35_06770"
     CDS             1284..>2477
                     /locus_tag="VFQ35_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9930368.1"
                     /translation="MNQRDHDRRSGHSSERDDNRREQRAGTDRRWSNERDEQDARADR
                     RVADHSDRFAGPGDRTSHEYGWNNERNWNDERNDWERHDDGVRAHSSEYGQDRWRPYS
                     GERMGPARGYDQSERTFRQSSSAGALPRGNWEHDRNDEQRSRGEHEHHGYGSPTEQGF
                     GAAQYGYGPAGYGSSGSNSGYGNRGERWQGSAGHGGERGQGGYGGERWQGGDRNERGQ
                     SYGGDRWRGAGEQGQGYGRNDHPGGRGFHGVGSYFGGNHGSYDSPEGNYGSDYGGPGA
                     GQRGSQRSGEQAFMGQGQRSHRGVGPAGYSRSDERIKEDVSDALTDHEEVDAKEIEVS
                     VKNGEVTLSGSVDARYMKRLAEDIAERVRGVRDVRNEIRVKQEATRGASPSSSQRGAG
                     TGANTA"
BASE COUNT          477 a          800 c          822 g          378 t
ORIGIN      
        1 agcttcccca tgggcgcaat cgtgaaccgc tcgctgacac tccgctcggg tcagtgccac
       61 gtgcaccgct atctcaagcc gttgctcgag cacattcagc gcggcgacat cgacccgagc
      121 ttcgtcatca cccatcgcct cagcctcgat gaagccgaca agggcttcga cctgttcctg
      181 cgcaatcggg acgagtgcga aaaggtcgtc ttgaaggcag cgtgaccgct ccatcacagc
      241 gagccatgta gaaagcgccg accggagact tctcctgtcg gcgctttccc tttactgaag
      301 gcctcgcggc gctcagtgct tgtaggcgct tccaggctcg gccattccgc ggtgttgctc
      361 ggcgagcgct tccttcggta ggaagtggga catcgcgacg cggagcttgt tttgccaacc
      421 ggtgacgaca tcgccctcac cgcgcatcat cgcgtcgaag ccttgcttcg cgacctcgct
      481 cgcgggctgc tttttatccg cggcgacctt cgtgtcgagc atgtccgcgc gatcgaagaa
      541 ctcggtctcg gtcgcgcctg gcatcaggca cgtgaccgtg acggcggagc ctttcagctc
      601 ggcgcgcaga gcgtaggcga acgaatccag gaacgccttg gtcccgttgt aaacggcctg
      661 gaacgtccct ggcatgaagc ccgcgatgga gcccgtgatc agaatgcgcc ccgcatcgcg
      721 gtttcgcatt tcgcggccga ccttctgaat caggtaaacg gtaccggtga cgttggtgtc
      781 gatcacgcgg cgcacgtcgt tccaatcttg atccagaaag gcgcggccga ggcctcgacc
      841 cgcgttggcg agcagcgcgt cgatgggacg accgcgcgcc gccgcgtaca gacggtccac
      901 gccctccgtc gtcgcgagat cggcttcgag cgactcgacc gcgacgcccg cacggcgcag
      961 ctcatcggcg gcggagtgaa tttcgggttc atcggcgacg atcaacaaat cgaagccgcc
     1021 ctctgcacag cacttcgcga gttcgtatcc gatgccagtc gatgcaccgg tgacgaccgc
     1081 aagtggacga gtttgagtat tcatcatttc taacctcctc ggagccttgc gacgggtgca
     1141 cgcaacgtgc cgcgtgcgcc gggagcaaag cggcgccgta atcgcgcgtg acctctccgc
     1201 gattgcgtgg gcgaggcacg cccgttgcag cggcgtttcc gagcgcgcag gtcgcgcgag
     1261 cattcaacgc agagggaatc ccgatgaatc aacgagatca cgacaggcgt tcagggcact
     1321 cttccgagcg tgacgacaac cgccgtgagc aacgcgccgg cacggaccgg cgctggagca
     1381 acgagcgcga cgagcaggac gcgcgcgcag atcgtcgcgt agcggatcac agcgatcgtt
     1441 tcgctggccc cggcgatcgt accagccacg agtacggctg gaacaatgag cgcaactgga
     1501 acgacgagcg taacgattgg gagcgccacg acgacggcgt gcgcgcgcac tcgagcgaat
     1561 acgggcaaga ccgctggcgt ccttatagcg gtgagcgcat ggggccagcg cgcggctacg
     1621 accagagcga acgcactttc cgccagagta gctccgccgg agcgctccct cgcggcaact
     1681 gggaacacga tcgcaacgac gagcagcgaa gccgcggcga acacgaacac cacggctatg
     1741 gctcccctac cgagcaaggt tttggcgctg cgcagtacgg ctacggaccc gctggttacg
     1801 gcagctctgg ctcgaactcc ggctacggca accgcggaga gcgatggcaa ggctccgccg
     1861 gtcatggcgg cgagcgcgga caggggggct acggcggtga acgctggcaa ggcggcgacc
     1921 gcaacgagcg aggacagagc tacggaggcg accgctggcg gggcgctggt gagcagggcc
     1981 aaggctacgg acgcaacgac catcccggcg ggcgcggctt ccatggcgtc ggcagctatt
     2041 tcggcggcaa ccacggctcc tacgacagcc ccgaaggaaa ctacggctcc gactacggtg
     2101 gccccggcgc ggggcagcgg ggctcgcagc gaagcggcga acaggccttc atgggtcagg
     2161 gacagcgctc acatcgcggc gtcgggcctg cagggtacag ccgctccgat gagcgcatca
     2221 aagaagacgt gtcggacgcc ttgacggatc acgaggaggt cgatgcaaag gagatcgaag
     2281 tcagcgtgaa gaacggcgag gtcacgctga gcggctccgt cgacgcgcgc tacatgaagc
     2341 gactcgccga agacatcgcc gaacgcgtgc gcggggtgcg cgacgtgcgc aatgaaatcc
     2401 gcgtgaagca ggaagccacg cgcggcgcct ctccgtcttc atctcaacgc ggagcgggaa
     2461 ccggcgcgaa cacggcg
//
LOCUS       DASTKS010000324         8172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_499670, whole genome shotgun sequence.
ACCESSION   DASTKS010000324 DASTKS010000000
VERSION     DASTKS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499670"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VFQ35_06775"
     CDS             complement(<1..1564)
                     /locus_tag="VFQ35_06775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930369.1"
                     /translation="MRLGLGGMASVWVARERGQISKRESLVAVKAMLPELAQQSDFRA
                     MFLEEGQIVRSIDHPNVVRVHEVGEQDGTLFMSMDWVEGDSLLAVIREAKKRRPIPPE
                     MAVKIISDAAAGLHAAHELRGWDGELRNLVHCDVSPHNILVGIDGRAKLVDFGVANAT
                     LTSSDDTERIKGKFSYMSPEQARGEKLDRRSDIFSLGIVLYELTTGERLFRGENAAHT
                     LALVTAAEIPDPRSVHSDYPARLADITLRALHRDPSRRYQSAKELRDALDRYLIEERV
                     MVSHAGVAQLVRRVIGARIESLRQSVREALVARDGSLAEGVIPEPPFSTISDASGPKS
                     PSSHSRTPRPQTYETPVPERSKFAPLLAALGGIAAAAASVVWMLHRPEFLESPSSNNA
                     EAPAAQSAPRAAPDAHSTTAASPNDPGVDIGSLPLAATANAPALLPRTFNGRAAARPR
                     GDSKSAKNDKHPEPLGIKLEEAAPVQTPAPAPQPTPTAAVPTTSGAVASFNRGAAMAQ
                     LGAAMGRAGACKR"
     gene            complement(1961..3742)
                     /locus_tag="VFQ35_06780"
     CDS             complement(1961..3742)
                     /locus_tag="VFQ35_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930370.1"
                     /translation="MLGAIACGAKQETRDSGLGLGGSPPASGGSNSLGGVTAEGGSTG
                     GSNPITLAGGPTCVSKTCKELGYACGSFQNDCGGITNCADEGLKCNSLEACLGGVDGP
                     TTCTSTIGPCDVCDTVPDCSKASQPTRLSGRVITAGRKDTETANQVGVPNAVVYVSRS
                     LDVGELPAIPSGIPADGMSCDRCEDQKLGPVLVGTTTDSTGRFTLEGTIPVGGEFMLV
                     VKVGRFRRAVKMTLPGSAACQTTMLPTTLPDNPTRLPRTMTDGLAVNIPRVAVSTGEV
                     DAMECVLEKMGIADSEFGNPGTQKRVHLYRGGRTAPGTGASIDSSTPLDSALYGDLSR
                     LLNYDILIADCEGSNWDQTFTQRDASGANVREYVNRGGRMFASHLSFSWLIGNGTGAY
                     DAQNPIATGLGTAADWDPMIDSTSATGNCVIASGRPQASPRIQNFSDWAAAEGVITPP
                     ATSFTIKQPRSSATRLGPSTEEFVYRTDGTARTQQFSFNTPYAAPSAAVCGRVAYSGF
                     HVAASDDLSGMQFADAVFPAHCAGTLTPQEKVLLYMLFDLGACVGTPPPPPMCTPTTC
                     KDANAKCGYIADGCGSVVDCGVCPPPR"
     gene            5081..>8172
                     /locus_tag="VFQ35_06785"
     CDS             5081..>8172
                     /locus_tag="VFQ35_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930371.1"
                     /translation="MAPVLSDGPLGLESNKVPPSESSRSGAGNSSEEFSHAVYAVPFG
                     RYEGAKPVEGRGSEVLIGRERERARMLDWLFTHGSRGAYLVTGYRGVGKTSFVNFCLR
                     QYDDDIYGRWLQSSAGRALFWDRLAVTGFFLAVPLSLLFASQLADILCALGQIGASPV
                     SLVRWGLVGLLTLSALYPFAHAISTARVAMRVAVPPDEKAAQTVGDDAPDSKNEKRDA
                     TANARRQRQSGYRRRLWVGFLGTAMPVLFLPLFRDPTNATAFFIALLTWTHAVCHATA
                     ISGERQLARSPASAGFSVCPPSSRSWAIIMSAVGAAVLFSLSLRWQGLQLLSLYWVIA
                     SASVTASFALRWYDSKQLVGFIKRLERGELEQWKDLREVLTARHSWGIYPTQLLLFAF
                     VWAYHDELASNLVVLGLADAAVVAVVGGVGLWRRRRAPSAVPASEGGAATEIKPWFAF
                     YPLPHLLMALRSLFAIALGVQVAPAIVELARLRFLRSVSASLVAPGGFLFAPSGCTSL
                     PTSLTAAARCHQRFGSAGEEVLWLLLVAIFASCLMYAEHDWVIRRHARARRDSASDPE
                     PSDDGKYAGAETRMELSVKTRELARATLFYSIFRTWLPLLTVRVNLGFDDLNHSHVVT
                     SMLVGLRNEFHRVYVSQRSRLGFLSSVVSVTAVALVALVAGEQLEMLRSPWSDDLRRV
                     PAFALTLADERDQRDHLLTHLLPYGPLSSVSGRWDERPTRLYLYWHQLAAFGLLFALA
                     RTFRGRINPLRKIHARLNAIAESTTSKIRRAEANARLRMSAPGVGLDASDRTAESETA
                     PLEPRAIEQTFLDLLRDMLEPHVALALEGHVISLPTPQITFIFDELDKVGVRSLDNLD
                     EQAATRGSASADHERSRRVHRLLADMKNLLSVAPARFIFVGGRDLHDEWLADQTARQP
                     LLSNIFDAEIYLQTLLTDRLGGNPSRRPCLTSGVDTYLAVQRERANALNDRWDKWRLG
                     PQLFDVGRSTPEHYFRQKLAPVDAGVFDAWPLYNANSWDGDGSDSLSFGAELLRHFSQ
                     "
BASE COUNT         1370 a         2612 c         2658 g         1532 t
ORIGIN      
        1 ggcgcttgca ggcgccggcg cggcccattg cggcaccgag ttgagccatc gcagcgccgc
       61 gattgaagct agcaaccgcg ccgctcgtcg tcggcactgc tgccgtgggc gtcggctgcg
      121 gcgcaggcgc tggcgtttgc actggcgcgg cctcttcgag cttgatgccg agcggctcgg
      181 ggtgcttgtc gttcttcgcg ctcttgctgt cgccgcgcgg ccttgccgcg gcgcggccgt
      241 tgaaagtgcg gggaagcaac gcaggtgcgt ttgcagtggc cgcgagcgga aggcttccta
      301 tgtccacgcc aggatcattc ggcgaggcgg cggtcgtcga gtgcgcatcg ggcgcagcgc
      361 gaggagccga ctgagccgcc ggcgcctcgg cgttgttcga cgagggagac tccaagaact
      421 cgggacgatg cagcatccaa accaccgaag ccgccgcagc ggcgatacct ccgagcgcgg
      481 cgagcagcgg cgcgaacttg gagcgctcgg gtaccggggt ttcgtaggtc tgaggacgcg
      541 gcgtgcgcga gtgggacgac gggctcttcg gtcccgaagc atcggagatg gtggagaacg
      601 gtggctcggg gatgacgccc tcggcgagcg agccgtcccg cgcgacgagc gcttcgcgga
      661 cgctctggcg cagcgattcg atgcgcgcgc cgatcacgcg gcgaacgagc tgtgcgacgc
      721 cggcgtgcga aaccatcacg cgctcttcga tgaggtagcg atcgagcgcg tcgcgcagct
      781 ccttggcgct ttgataacgc cgggagggat cgcggtgcag cgcgcgcaag gtgatgtccg
      841 cgagccgggc ggggtagtcg gagtgaacgg agcgagggtc ggggatctcc gccgcggtca
      901 cgagcgctag cgtgtgcgcc gcgttttcgc cgcggaacag gcgctcgccg gtggtgagct
      961 catacagcac gatgcccagc gaaaagatgt cgctgcgacg atcgagcttt tcgccgcgag
     1021 cttgctccgg cgacatgtac gaaaattttc ctttgatgcg ctccgtgtcg tcgctgctcg
     1081 taagggtcgc gttggcgaca ccaaaatcga cgagctttgc gcggccgtcg atgcccacca
     1141 aaatgttgtg cggcgaaacg tcgcagtgaa ccaaattgcg gagctcacca tcccaaccgc
     1201 gcagctcgtg cgcagcgtgc aagccggcgg cagcatcgga aatgatcttt actgccatct
     1261 cgggagggat cggacggcgc ttctttgcct cgcggatcac cgcgagcagc gaatcgccct
     1321 cgacccagtc catcgacatg aacaaggtgc cgtcctgctc gcccacctcg tgaacacgca
     1381 cgacgttcgg gtgatcgatc gagcgcacga tctgcccctc ttcgaggaac atcgctcgga
     1441 agtcggattg ttgagcgagc tcgggcagca tcgccttcac cgcgaccaga ctctcgcgct
     1501 ttgaaatttg accccgttct cgcgcgaccc aaacactcgc catgccgccc aggccgagcc
     1561 tcactaggag ctcgtaacgc ccaagcactg aacctgacga caattcgctc atctggcgag
     1621 ccgcggagtg ggcacttcca ctcaccggct ctcaccatgc catggaagtt cggcgcaagc
     1681 gaggctcgcc gagtctcgtt taatgccgcg aaactgcggg atttcactgc agtgtgggac
     1741 aagcgtctac ccatacacgc gcgtgtacga gagacgttcg ctgcaacatt ccaatcaagt
     1801 tagagcggct tcttcggcgc gctcggaagg atcagtgcac gggacgattc ggacgattcg
     1861 cttcggtcgg ggcagccaat gcgcgccccg cgcaagcgat ccaccaaagt acgacgaccg
     1921 aaagctcatc cacgggttgg cccaaaccca cggagcgcca tcaccttggc ggcgggcaga
     1981 ccccgcagtc gaccacgctg ccgcaaccat ccgcgatgta gccgcacttg gcgttcgcgt
     2041 ccttgcaggt cgttggggtg cacatcggag ggggcggggg cgtgccgaca caggcgccga
     2101 ggtcgaagag catgtagagg agcaccttct cctgcggtgt gagggtgccg gcgcaatgcg
     2161 cggggaatac cgcgtcggca aactgcattc cactgaggtc gtcgctcgcc gcgacgtgaa
     2221 agccgctgta cgcgactcgc ccgcatacgg ccgcgctcgg cgccgcatag ggggtattga
     2281 acgagaactg ctgcgtgcga gcggtcccgt ccgttcgata gacgaattct tccgtagaag
     2341 ggccgaggcg tgtagcgctg ctgcgcggct gcttgatcgt gaagctcgtt gccggcggcg
     2401 tgatcacgcc ttccgccgcc gcccaatcag aaaagttctg gatgcgcggg ctcgcctgag
     2461 gcctgccgct ggcgatgacg cagttgcccg tcgcgctcgt cgaatcgatc atcggatccc
     2521 agtcggccgc cgtgccgagc cccgtcgcaa tggggttttg agcgtcgtag gcgcccgtgc
     2581 cgttgccaat caaccagctg aaggacaggt ggctggcgaa cattcgccca ccacgattca
     2641 cgtactcgcg aacgttcgcg ccagaagcgt cgcgctgcgt gaatgtctgg tcccaattcg
     2701 agccctcgca gtcggcaatc aagatgtcgt agttcaagag gcgactgagg tcgccgtaca
     2761 gcgccgaatc caatggcgtg ctcgagtcga tgctggcgcc agtgccgggt gccgtgcgcc
     2821 ctccgcggta caagtggact cgcttctgtg tgccggggtt cccgaactcg gagtccgcga
     2881 tgcccatctt ctcgagaaca cactccatgg cgtcgacctc gccggtcgag acggcgacgc
     2941 gcgggatatt gaccgcgagc ccgtccgtca tggtgcgcgg caagcgtgtc ggattgtccg
     3001 gcaacgtggt tggcagcatc gtggtctggc aggcggccga accgggtaac gtcattttca
     3061 cggcgcgccg gaagcgcccg accttgacca cgagcatgaa ctcgccgccg actggtatcg
     3121 tgccttcgag ggtgaaacgc ccggtggagt cggtcgtggt gccgaccagc acggggccga
     3181 gcttctgatc ttcgcaacgg tcacagctca ttccgtcggc gggaatgccg gacgggatgg
     3241 cgggcagctc gccaacatcg aggctccgcg agacgtagac cacggcgttc ggtactccga
     3301 cttggttggc ggtctcggtg tcttttcggc ctgccgtgat cacccgcccg ctcaagcgcg
     3361 tcggctgact cgccttgctg caatccggca ccgtgtcgca gacatcgcaa ggaccgatgg
     3421 tcgaggtgca ggtcgtaggc ccgtccactc cgccaaggca tgcttcgagt gagttgcact
     3481 tgagaccctc gtccgcacaa ttggtgatcc cgccgcaatc gttctggaac gagccacacg
     3541 cgtagccgag ctccttgcac gtcttcgaga cgcacgtcgg cccccccgcg agcgtgatcg
     3601 gattcgagcc gcccgtactg cctccctccg cggtgacccc gccgagagaa ttggagccgc
     3661 cgctcgccgg agggcttccg ccgagcccca gcccggagtc tctggtttct tgtttggcac
     3721 cgcaggcgat agcgccaagc acgaccatgc caactaccgc gcgccgaaac tcaaccgccc
     3781 tggaatgcat cgaattcacc tgcctgaaaa atagagagtc gacggatgac acacttcgcg
     3841 gcctcgccgg agcgccgagc ccgcgtgatc aaaccacgca cgggagcccg cgtgatcaaa
     3901 ccacgcgcgg gagcgccgcg ctcgccccac ggctgaactc gttgaggtgg cgtcgcgacc
     3961 ccccaggttc cgaagccgcc cgcgcggaac tttgtagggt gattgtgcgt ggccgagcgg
     4021 ggaggtcgcg caaggccaca gacttcgagc tccgggttcc ttcgcggtca tggctctagg
     4081 gtgccgaggc gcgctggaat gaggcagcag aatggcaaag ccactcgctt gccctcgaaa
     4141 cggcacggaa cggccgcgac gagcaggctc gggacgccgc ttgggcctct ccggcgcgtt
     4201 gtcttcaggc gcacccgcgt cgaaaggcgc gctgacgggc ctcgcttcac gggtatctcg
     4261 ccgatcgagc ttggttaacg gcggcgtgac gttctggcgc cttggtgggc tccgacgatt
     4321 ctgagcagtt cgcgagcggt gcgagcgagg gcctcggcaa ctccgaaatg attgcgagcg
     4381 gtgcgagcga gggcctcggc aactccgaaa gagcacatag tcgtgattgc acctgaacgg
     4441 ttcggccgcg ccctggggcg tacgcgaacg cgcgcgcaaa gggacgaccg cgatgcaagc
     4501 acgcacgcag tatcctcagt cattgcccga ccaagcatcg atgttgacgc ggagagtcgc
     4561 ggcgaagtcg acttggcggc gggtgctgca gcgaagcgct ctcgttcctc cacgtcgacg
     4621 tcccgtgctc tgctcggcga gctcgaggta ttgagctttc cacgtccgga tgtcggctca
     4681 ccgcgcttct ttcctccgcc gagtacgcgg tcgccacgtc atgatcatgg gccacgcgca
     4741 cgcgcagatg gcagccttgc gccgcacgtc tctccgcacg atcgcaatca agtagcggcc
     4801 gccttcacaa gctgcgcgtt tccggtcggg gtgagctcct cgcacgcttg gcacgcgggc
     4861 acttcggacc gtggctcgcg cagaaccaga acgacgtctt gtcgcaagct gctcccatcc
     4921 gccaagcgct ggcggccagc agcgcacttg gcccgcgaat cttgcgggta cctgcaagcg
     4981 gtcgaagatg tagtcattca ttcggttcct gtaacgtttt gggcaaacgt tgaatgaaca
     5041 aagattcgat gttccctggt acgctgttgc taaggtgcat atggctcctg tgctcagcga
     5101 tgggccgcta gggctcgagt ccaacaaagt tccgccgtcc gaatcgagtc gctccggagc
     5161 tggcaattcg agcgaagagt tctctcacgc cgtctatgca gttccattcg gccgatacga
     5221 aggcgcaaag ccggtcgaag gtcgtggcag cgaagtgctg atcgggcgtg agcgcgagcg
     5281 cgcccggatg ctcgactggc tgttcacaca cggcagtcgc ggcgcgtact tggtcactgg
     5341 atacagaggt gtaggaaaaa cgagcttcgt gaacttctgc ctgcgccaat acgacgacga
     5401 catctacggc cgttggttgc aaagctcagc ggggcgagcg ttgttttggg atcgccttgc
     5461 cgtgacgggc tttttcttgg ctgttccgct gagccttctg ttcgcttcgc agctcgcgga
     5521 catcctgtgc gccctcggac aaatcggagc ttcgcccgtg tcgctggtcc gttggggtct
     5581 cgttgggtta ctcacactga gtgccctgta tcccttcgca cacgcgattt cgacggcgcg
     5641 ggtggcgatg cgcgtcgcgg tgcctcccga tgaaaaagcg gcgcagacgg tcggagacga
     5701 cgctccggat tctaagaacg agaaacggga tgccactgcg aacgcgcgac gtcagcgcca
     5761 atcgggctac cgacgacggc tctgggtcgg ctttctcggc acggcgatgc cggtcctttt
     5821 tctccctttg tttcgcgatc caacgaacgc caccgccttc ttcatcgccc tgctgacttg
     5881 gacgcacgct gtctgtcacg cgaccgcgat ctcgggcgaa cgtcagctgg cgagatctcc
     5941 agcgagcgcg gggttttcgg tgtgcccgcc ttcgagtcga agctgggcga tcatcatgtc
     6001 cgccgtgggg gcagcggttc tgttctcgct ttcgctgcgg tggcaaggcc tccagcttct
     6061 ctccttatac tgggtcatcg ctagcgcgag cgttaccgcc tcctttgcgc ttcggtggta
     6121 cgactcgaag cagctagttg gcttcatcaa gcggctcgag cgaggcgagc tcgagcaatg
     6181 gaaggatctg cgcgaggtgc tcacggcgcg ccacagttgg gggatctacc cgactcaatt
     6241 gttgctgttc gcgttcgtgt gggcatatca cgacgaactc gcgtctaact tggtcgtgct
     6301 gggcttggcc gacgccgctg tggtcgcggt cgtcgggggc gttgggctct ggcgcaggcg
     6361 gcgcgccccc tcggcggtgc cagcaagtga aggcggcgcg gcaaccgaaa tcaagccttg
     6421 gttcgcgttc tacccgctgc cgcatttgtt gatggcgctc agaagcctgt tcgcgatcgc
     6481 cctcggggtt caagttgcgc ccgcgatcgt cgaattggcc cggctcaggt ttctccgttc
     6541 ggtgtcagcc tcgcttgttg cgcccggcgg cttcctcttc gccccgagcg gctgcaccag
     6601 cctcccgact agcctcactg cggcggctcg ttgccaccag cgtttcggtt cggcgggcga
     6661 agaagttctt tggctcctgc tggtcgccat cttcgcgtcc tgtctcatgt acgccgaaca
     6721 tgactgggtc attcggcgtc acgcacgcgc ccgccgcgac agcgcgagtg atccggagcc
     6781 gtcggacgac gggaagtatg caggggccga gacgcgcatg gagttgtcgg tgaagacgcg
     6841 cgagctcgcg cgcgcgaccc tcttctactc catcttcaga acctggctcc cgctcctgac
     6901 cgtgcgggtc aatctcggct ttgacgacct gaatcacagc cacgtcgtca cgagcatgct
     6961 ggtgggactg agaaacgagt ttcatcgcgt ctacgtttcg cagcgcagcc gtttgggctt
     7021 cttgtcgtcg gtggtctcgg tgactgcggt cgcgttggtg gcgttggtcg ctggcgaaca
     7081 gctcgagatg ctgcgcagcc cttggagcga tgacttacgg cgcgtgccgg cgttcgcgtt
     7141 gacgctcgcc gacgagcgcg accagcgcga tcacctcctc actcatctgt tgccgtacgg
     7201 ccctttgagc tcggtcagcg ggcgctggga cgagcgaccg acgagactct atttgtactg
     7261 gcatcagctg gctgcattcg ggctcctgtt cgcgctggcg cgcacgttcc gtggccggat
     7321 caacccgctg cgcaagatcc acgcgcggct caatgcgatc gccgagtcga ccaccagcaa
     7381 aatacgcagg gccgaggcga acgcacgcct caggatgtcg gcgccgggcg tcgggctcga
     7441 tgcttcggat cgcaccgccg agagcgagac ggctccgctc gagccccgcg cgatagagca
     7501 gacattcttg gatttgttac gagatatgct cgagccgcat gtcgcgctgg cgctcgaggg
     7561 gcacgtcatt tcgctgccga cgccccaaat taccttcatt ttcgacgaac ttgacaaagt
     7621 cggcgtgcgt tcgctcgaca acctcgacga acaagccgcg acgcgaggca gcgcgtcggc
     7681 cgaccacgaa cgctcgcgac gcgtccaccg cttgcttgcc gacatgaaga acctcctctc
     7741 cgtcgcgccg gcgcgcttca tcttcgtagg ggggcgcgac ttgcacgacg agtggctcgc
     7801 cgatcaaacc gcgcggcagc cgctattgtc caatatcttc gacgccgaaa tctacctcca
     7861 aactctgttg acggatcgcc tgggcggcaa tccgtcgcgt cggccgtgct tgacgagcgg
     7921 cgtcgatacc tatctggcgg tgcaacgcga gcgggcgaac gcgttgaatg atcgctggga
     7981 caaatggcgc ctcgggcctc agctcttcga tgtcgggcgc agcacgcccg aacactattt
     8041 caggcagaaa ctcgcgcccg tcgacgcggg cgtctttgac gcctggccgc tctacaacgc
     8101 caattcttgg gacggcgacg gcagcgatag cctatcgttt ggggccgagt tgctgcggca
     8161 cttcagccag ta
//
LOCUS       DASTKS010000325         7805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1614894, whole genome shotgun sequence.
ACCESSION   DASTKS010000325 DASTKS010000000
VERSION     DASTKS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614894"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFQ35_06790"
     CDS             <1..358
                     /locus_tag="VFQ35_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930372.1"
                     /translation="RAKDGKEHSLLAPKNAEVAFAGSIKRKDGEPEKFGDKREGDREI
                     ALKGEKPVHLTRSWPVAGQKPIANGDELELMVGIWGVGENAASRRPLKKFCKVTLKFE
                     KDKPRVVVTTPDGVSK"
     gene            complement(385..1155)
                     /locus_tag="VFQ35_06795"
     CDS             complement(385..1155)
                     /locus_tag="VFQ35_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930373.1"
                     /translation="MSWLRAASSAGLVVLLGAQACGEDSNAKPTGSAGAGGKGGASAS
                     TTGGGENTGAGGDGLHVAEGGAAFRCRATSCEPETLVGECPATAPGIYDPCSTFGSSC
                     VYCASADTCTESQEPFIFQCCMFDGKLSWSHNCLRQCPLSELEKRDILGHDGGGGAGG
                     ANAIASAVGADPAQGCVLARPSACCVLDQDCPTGFECARSADNGGASACKPMLADASR
                     CWQSSDCGGDGTCEGALFCSCTTECNDEVQGYCAYGPR"
     gene            complement(1232..2008)
                     /locus_tag="VFQ35_06800"
     CDS             complement(1232..2008)
                     /locus_tag="VFQ35_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9930374.1"
                     /translation="MSDVSAERRDGIALVHLDRPDRMNALSRNLLLELEALGAELEND
                     TALRAVVITGRGERAFSAGADLKERVGLNEADVLELLHLFRRAFAWLDRLPVPSVAAI
                     NGVALGGGLELALACDLRVAHESALLGLPETSLAIIPGAGGTQRLPRIVGRAKATELI
                     LLGRRLNAAEALEIGLVHRVVPANADFIAATLEYVEPIVSGAPIAQRAAIQALRASEL
                     PLSEGLAAELDAYESCLYSEDRREALRAFQEKRKPRFQGR"
     gene            complement(2005..4647)
                     /locus_tag="VFQ35_06805"
     CDS             complement(2005..4647)
                     /locus_tag="VFQ35_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9930375.1"
                     /translation="MTSAHRLHERCAASALASAPFALAGTERRYERARPFLVRHTVLD
                     LSLDFEERSVTGTARLSIARVVEGAETMALDAIGFELGSVRVIGATGARDVGYEYDGA
                     TLRVHGLTESELTLEVSYRAVPRRGLYFLAPDPKVKDRPVQVWTQCQDEDARHWFPCH
                     DKPHVKMTTEIRVAVPKGFVALSNGEPVESKASKGATSASVFHFKLDKPHPSYLVTLV
                     VGKFDIVEDRPAVLEDGREIKVVYYVPQGRRGDAKRSFGQTPRMIELFSKVTGVPFPW
                     SRYSQVVVSDFVFGGMENTTATTMYEHVMVDARAELDISSHDLVAHELAHQWFGDFVT
                     CRDWPQAWLNEGFATYFEHVEREDRLGRDEYDYGVATDMDTYLSEASGRYQRPIVCRE
                     YHEPIDLFDRHLYEKGGLVLHMLRRKLSDSAFFAGIKHYLSAHAHGLVETVDLQRALE
                     QSSGLPLDRFFDQWVLRPGHPELKVKVGYEDELLTVSVKQAQKGNDVTIFDFDFEIEV
                     RSADGDVSRHRKHITSAQESLVVKLPKRPSYVGIDPELRVVGSLSLDVPNDLLKNQLE
                     HGSSARLRWAAAEALAKKPDITSVAALGRVLGNEDELWMVRAEAARALGKTRLPDAQA
                     ALAERTHVGHPKVRRAVASALGAFKNETSLKALLPLAKKDASFLVESEAARSLGKTRQ
                     AAALKPLLGMLERDSWGDVVRQGALDGLGALRDEGGLDAVRAHTEYGHPTRGRRSAIS
                     ALAELGEGKHTREALEALLEDADPHLRIEVINALVRFADPKSRGALRRTLDHELDGRV
                     VRRLREALRDLSDGSGERKRVNDELESLRNELSELKHRLSELEEKKKPIDEKKKPTDE
                     KKKQAESHKPNKPTPRKKAKSRRR"
     gene            4740..6926
                     /locus_tag="VFQ35_06810"
     CDS             4740..6926
                     /locus_tag="VFQ35_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET9930376.1"
                     /translation="MPITLSGEVERVTYESESTSFRVLRVGSLEGEGARSGSIAVVGT
                     FQAVGPGTRIRITGNFVTDARHGEQLRADVVVPIEPNTLVGLERYLGSGLIPGVGSAL
                     AKRIVEAFGLETLRVLDEQPDRLASVPGLGERRVKDIKASWKAQRALSSVLVLLQSHG
                     ASHNLALRIWKHYGDRAASIVQRSPYRLALEVKGVGFKTADRLAHAFGISHDHPERCQ
                     AGVIHMLEEFSEAGHVYVPREELVPRVAEMLEVGEPHIEVALDALYASERIVLDGRAV
                     YLTYLHRAELEVTAAVQRLCSQPAKAIPGAETAIEAFEKRGNLKLAPSQRSAVELAVS
                     ERIAIITGGPGVGKTTIIRAIVSVFETKRRAVRLAAPTGRAAKRLSEATGHEASTLHR
                     LLEFEPRSAEFQRGPERPLEADVVIVDEASMIDLPLMRSLLLALPPAARIVIVGDADQ
                     LPSVGPGAVLRDLLESRVVPTVRLSEVFRQAETSAIVVNAHVILRGEMPETTSADSPR
                     GDFFIVPRKTAEEAAETIRELVAVRIPRRFGLDPRKDIQVLTPMHRGPAGTAALNEVL
                     QRALNPEGPALEVRGQKLRLGDKVIQTRNDYENEVFNGDVGVITAVDTEERKLSVRFD
                     NNEVVYEEGEVEALTLAYAMSVHKSQGSEYPAVVFPLLTSHFVMLAKSLLYTGVTRAR
                     KLCVLVADPKALSLAVSDARREERRTRLAERIAFGSASPRAAVSTS"
     gene            complement(6929..>7805)
                     /locus_tag="VFQ35_06815"
     CDS             complement(6929..>7805)
                     /locus_tag="VFQ35_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9930377.1"
                     /translation="AELQRLVGAANGQVVNEVVEDQQSTRGASLSIRVPSNTVQAFVA
                     DLGRLGKIRSQKLETHDVSRTVNDAEILLSNLERALERYQELLAKAANVTEALAIEAE
                     LQRVRASLERVRGDLEWMRDRVARSTVYVTLSLEDGAATLQNEAKVHPGVRGVMLFDV
                     PPSGGGRSFAGAGLSLGWGRSFSLDLDLMKRIDAHASGSINSALLTMGGELYSDFLGG
                     GRRRWLNPYFGLRLGYGRLLGENALVLGGDLGVEIVKTDFFMLELQGRAHALIGPDDG
                     VHVGIEPIVGMSFAY"
BASE COUNT         1266 a         2541 c         2674 g         1324 t
ORIGIN      
        1 gcgcgcgaaa gacggcaaag agcactcgct gctcgcgccg aaaaacgcgg aagtcgcctt
       61 cgcgggcagc ataaagcgca aagacggcga acccgagaag ttcggggaca agcgcgaagg
      121 cgaccgagaa atagcgctga agggcgagaa gccggtgcac ctgactcgca gctggccggt
      181 cgcaggtcag aagccgatcg cgaacggcga cgagctcgag ctcatggttg gcatttgggg
      241 cgtcggtgaa aacgcggcgt cgcggcgccc cttgaagaag ttctgcaagg tgacgctcaa
      301 gttcgagaag gacaagccgc gcgtcgtcgt gacgacgccg gacggcgtct cgaagtagcg
      361 ggagacggtg ggcgttcgct gcagctagcg cggaccgtaa gcgcagtagc cttggacttc
      421 gtcgttgcac tcggtcgtgc acgagcaaaa cagggctccc tcacaggtgc cgtctccgcc
      481 gcagtccgag ctctgccagc agcgagacgc atcggcgagc atcggtttgc atgccgatgc
      541 gccgccattg tctgctgagc gagcgcactc gaacccggtc gggcagtctt ggtcgagaac
      601 gcagcacgcg gagggtcgcg ctagcacgca gccttgcgcg gggtcggcgc caactgcgct
      661 agcgatcgcg ttcgctccgc ccgcgccccc gccaccgtcg tgtcccaaga tgtctctctt
      721 ctcgagctcc gaaagcggac actgccgcag gcagttgtga gaccagctga gcttgccgtc
      781 gaacatgcag cactgaaaga tgaacggctc ttggctttca gtgcacgtgt cggcgctggc
      841 gcagtagacg cagctgctgc cgaaggtcga gcaagggtcg tagatgcccg gcgcggtcgc
      901 tgggcattca cctaccaagg tttctggttc gcagctggtt gctcggcagc gaaaagcagc
      961 gccgccctcg gcgacgtgga gcccgtcgcc gccagcgccg gtgttctccc ctccgcccgt
     1021 cgtcgatgcg ctcgctccgc ccttcccgcc agcaccggcg ctgcccgtgg gcttcgcatt
     1081 tgaatcttcg ccgcaagcct gtgctccgag cagcacgacg aggcccgctg aggaggcggc
     1141 gcgaagccaa ctcacgatca tgcaagttat ctagcaggga gcgcgcgcgt cggaaaacgg
     1201 atgtttcggt agcgtagtcc ggcgcgcagc gtcagcggcc ttggaaccgt ggttttcgct
     1261 tttcctgaaa ggcgcgcagc gcttcgcggc ggtcctcgct gtacaaacac gattcgtaag
     1321 cgtcgagctc tgcggcgagc ccctcggaca agggtagctc cgaggcgcgc agggcctgga
     1381 tcgcggcgcg ctgcgcgatg ggcgcgccgc tcacgatggg ctcgacgtac tcgagcgtgg
     1441 ccgcgatgaa gtcggcgttt gcgggcacca cgcggtgcac gagcccgatt tccagcgctt
     1501 cggcggcgtt caagcgccgc ccgagcaaga tgagctcggt ggccttggcg cgccccacga
     1561 tgcgcggcag gcgttgggta ccgcccgcgc cggggatgat ggcgagcgac gtctcgggca
     1621 ggccgagcag agcggactcg tgggcgacgc gcagatcgca ggccagcgcg agttcgagcc
     1681 cgccgccgag cgcgacgccg ttgatggctg cgacggaggg aaccggcagt cgatcgagcc
     1741 aggcgaaggc gcgacggaac aggtgcagca gctcgagcac gtccgcttcg ttcagcccca
     1801 cgcgctcctt gagatcggcg ccggcggaga aggcgcgctc gccgcggccc gtgatcacga
     1861 ccgcgcgcag agcagtgtcg ttctcgagct ctgcgccgag cgcttcgagc tcgagcaaca
     1921 ggttgcgcga cagcgcgttc attcgatccg ggcgatcgag atggacgagg gcgatgccgt
     1981 cgcgacgttc ggcgctgacg tcgctcatcg acgcctcgac ttggccttct tccggggggt
     2041 cggcttgttc ggcttgtgag actcggcctg tttcttcttc tcgtcggtgg gcttcttctt
     2101 ttcgtcgatg ggcttctttt tctcttcgag ctcgctcagc cggtgcttca gctcggagag
     2161 ctcgttgcgc aggctctcga gctcgtcgtt gacccgcttg cgctcgccgc taccatcgct
     2221 gagatcgcgc agcgcctcgc gcagacgacg cacgactcgc ccgtcgagct cgtggtcgag
     2281 agtgcggcgc agcgctccgc ggctcttcgg atctgcgaag cggacgagcg cgttgattac
     2341 ttcgatgcga aggtgtgggt cggcgtcttc gaggagcgct tcgagcgctt cgcgcgtgtg
     2401 cttgccttca ccgagctctg ccagcgccga gatggccgaa cggcgcccac gcgtggggtg
     2461 tccgtactcg gtgtgcgcac gcacggcatc gagcccgccc tcgtcgcgca gcgcgccgag
     2521 cccgtcgagt gcgccttggc gtaccacgtc gccccacgag tcgcgctcga gcatgccgag
     2581 cagcggcttc aaagcggcgg cttggcgcgt cttcccgaga gagcgcgcgg cttccgattc
     2641 gaccaagaag cttgcgtctt tcttggcgag ggggagcaac gccttcagcg acgtttcgtt
     2701 cttgaacgcg ccgagcgcgc tcgccacggc gcggcgcact ttgggatggc cgacgtgcgt
     2761 gcgttcggcg agcgctgctt gagcgtcggg caatcgcgtc ttgccgagcg cgcgggcggc
     2821 ttcggcgcgg accatccaga gctcgtcctc gttgccgagt acccgaccga gcgcggccac
     2881 gctcgttatg tccggcttct tggcgagcgc ctcggcggct gcccagcgca gccgcgcgct
     2941 gctgccgtgc tcgagctggt tcttcaagag atcgttcgga acgtccaggc tcaacgagcc
     3001 gaccacgcgg agctccgggt cgatgccgac gtagctcggg cgcttcggca gctttacgac
     3061 caggctctcc tgcgcgctcg tgatgtgctt gcgatggcgg gagacgtcgc cgtcggcgct
     3121 gcgcacctcg atctcgaagt cgaaatcgaa gatggtcacg tcgttgccct tttgcgcttg
     3181 tttgacgctg accgtgagga gctcgtcttc gtagccgacc ttgactttga gctcggggtg
     3241 gccgggccgg agcacccact gatcgaagaa ccgatcgagc ggtaggccag agctctgctc
     3301 gagtgctcgc tgcagatcca ccgtttcgac gagcccgtgc gcgtgcgcgc tcaagtagtg
     3361 tttgatgcct gcgaagaagg cggagtcgct gagcttgcgc ctaagcatgt gcaagacgag
     3421 gccgcctttt tcgtaaaggt gccggtcgaa caagtcgatt ggctcgtggt actcgcggca
     3481 cacgatgggc cgctgatagc gaccgctggc ctccgacaag taagtgtcca tgtcggtggc
     3541 gacgccgtag tcgtactcgt cgcggccgag ccgatcttca cgctcgacgt gctcgaaata
     3601 agtcgcgaag ccttcgttca gccaggcttg gggccagtcg cggcaggtga cgaagtcgcc
     3661 gaaccattgg tgagcgagtt cgtgggccac gaggtcgtgc gagctgatgt cgagctcggc
     3721 gcgcgcgtcg accatgacgt gctcgtacat ggtcgtcgcg gtcgtgttct ccatgccccc
     3781 gaatacgaag tcgctgacca cgacctgcga atagcggctc caagggaagg ggactcccgt
     3841 gaccttcgag aagagctcga tcatgcgcgg cgtctggccg aagctgcgct tggcatcgcc
     3901 ccgccgacct tggggcacgt agtagacgac cttgatttcg cggccgtctt cgaggacggc
     3961 aggtcgatct tcgacgatgt cgaacttgcc gacgacgagg gtcaccaagt agctcgggtg
     4021 gggcttgtcg agcttgaagt ggaacaccga ggcgctcgta gcgcccttgc tcgccttgct
     4081 ttcgacgggt tcgccgttcg acaacgccac gaagcccttg ggtacggcga cgcggatctc
     4141 cgtcgtcatc ttcacgtgag gcttgtcgtg gcaggggaac cagtgccgcg cgtcctcgtc
     4201 ctgacactgc gtccaaacct ggacgggccg atccttcacc ttcgggtcgg gcgccaaaaa
     4261 gtagagcccg cgtcgcggaa cggcgcggta gctcacctcg agtgtgagct ccgactcggt
     4321 cagcccatgc acgcgcagcg tggcgccgtc gtattcgtag ccgacatcgc gcgcgccggt
     4381 cgcgccgatc acgcgcaccg agccgagctc gaaaccgatg gcgtcgagcg ccatcgtttc
     4441 tgccccttcg acgacgcgcg cgatgctgag gcgcgcggtg cccgtcaccg atctctcttc
     4501 gaagtcgagc gagaggtcga ggacggtgtg gcggacgagg aaggggcggg cgcgttcgta
     4561 gcgtcgctcg gttccggcga gggcaaacgg ggcggaggcg agcgcgcttg cggcgcaacg
     4621 ctcgtgaagg cggtgagcgc tagtcattct cccccgaatt accggctctt cggcgccagg
     4681 gcaagcgcgg gaaggcgggc cggctcaccc tctcggctac ctgacgctat agtgccgcgg
     4741 tgcccatcac gctcagcggc gaagtcgagc gcgtaaccta cgaaagcgag tccacgagct
     4801 ttcgagtgct gcgcgtgggc tcgctcgagg gcgagggggc gcggagcggc agcatcgccg
     4861 tggtggggac gtttcaggcg gtggggccag ggacgcgaat tcgcatcacc ggcaatttcg
     4921 tgaccgacgc gcgccacggg gagcagctgc gcgccgacgt cgtggtcccg atcgagccaa
     4981 acaccttggt ggggctcgag cgctacctcg gctcggggct gattcccggc gtcggtagcg
     5041 cgctcgcgaa gcgcatcgtc gaagccttcg ggctcgagac gctgcgcgtg ctcgacgaac
     5101 agccggatcg cctggcgagc gtgcccggcc tcggtgagcg ccgcgtcaaa gacatcaagg
     5161 cgagctggaa ggctcagcgc gctctcagca gcgtgctcgt gttattgcag agccacggcg
     5221 cgtcacacaa cctcgctctt cgcatctgga aacattacgg cgacagggcc gcgagtatcg
     5281 ttcagcgctc gccctatcgc ctcgcgctcg aggtgaaggg cgtcggcttc aagacggcgg
     5341 atcgcctggc gcacgctttc ggcatctcgc acgatcaccc ggagcgctgc caggcgggtg
     5401 tgatccacat gctcgaagag ttttccgagg cgggccacgt ctacgtgccg cgcgaagagc
     5461 tcgtgccgcg cgtcgccgag atgctcgagg tcggcgagcc gcacatcgag gtcgcgctcg
     5521 acgcgctgta cgcgagcgag cgcatcgtgc tcgacggccg cgccgtgtac ctgacgtacc
     5581 tgcaccgcgc cgagctcgag gtgaccgcgg cggtacagcg gctgtgctcg cagccggcaa
     5641 aggccatccc cggcgccgag accgcgatcg aggccttcga aaagcgcggc aacctgaagc
     5701 ttgcgccgag ccagcgcagc gcggtcgagc tcgccgtgag cgagcgcatc gccatcatca
     5761 cgggtgggcc tggcgtcggc aagaccacca tcattcgcgc catcgtctcg gtgttcgaga
     5821 cgaagcggcg cgcggtgcgc ctggccgctc cgaccgggcg cgccgcgaag cgtctgtccg
     5881 aagcgaccgg gcacgaagcg agcacgctgc accgcctgct cgagttcgag ccgcgcagcg
     5941 ccgagttcca gcgcggcccg gagcgtccct tggaggccga cgtcgtgatc gtcgacgagg
     6001 cgtcgatgat cgacttgccc ctgatgcgct cgctgctctt ggcgttgccg cccgcggcgc
     6061 ggatcgtcat cgtgggcgac gccgatcagc tgccgagcgt gggccccggc gcggtcctgc
     6121 gcgatctgct cgagagccgt gtggttccga ccgtgcgcct gtcggaggta tttcgacaag
     6181 cggagacgag cgcgatcgtg gtgaacgcgc acgtgatttt gcgcggggag atgcccgaga
     6241 cgacgagcgc cgattcgccg cgcggcgact tcttcatcgt gccacggaag accgccgaag
     6301 aggcggccga gacgattcgc gagctcgtcg cggtgcggat cccgcgccgc tttgggctcg
     6361 accctcgcaa agacatccag gtgctcacgc cgatgcaccg cggcccggcg ggcacggccg
     6421 ccctgaacga agtgctccaa cgcgcgctca atccggaagg acctgcgctc gaggtccggg
     6481 ggcagaagct ccggctcggc gacaaggtga tccagacgcg caacgactac gagaacgagg
     6541 tcttcaacgg cgatgtcggc gtcatcaccg cggtcgacac cgaagaacgc aagctcagcg
     6601 tgcgcttcga caacaacgag gtcgtgtacg aagagggtga ggtcgaagcc ttgacgctcg
     6661 cgtacgcgat gagcgtgcac aagagccaag gcagcgagta cccagcggtg gtgttcccgc
     6721 tgctcacgag ccacttcgtg atgctcgcga agagcttact ttacaccggc gtcacgcgcg
     6781 cgcgaaagct ctgtgtcctc gtcgcggatc cgaaggcgct ctcgctcgcg gtttcggacg
     6841 cgcgccgtga agagcgtcgc acacgcctcg ccgagcgcat cgcattcggt agcgcatcgc
     6901 cgcgcgccgc cgtgagtacc tcctgagctc aataagcgaa gctcatcccg acgatcggct
     6961 cgatgccgac gtgaacgccg tcgtcggggc caatcagggc gtgcgcgcgg ccctggagct
     7021 cgagcatgaa gaagtcggtc ttcacgattt cgacgcccag atctccgccg agaaccaagg
     7081 cattttctcc gagcaagcgc ccgtagccca accgtaagcc gaaatatggg ttgagccagc
     7141 gccggcggcc gccgccgaga aaatccgaat aaagctctcc gcccatcgtg aggagcgcgc
     7201 tgttgatgct tccggaggcg tgcgcgtcga tgcgcttcat cagatcgagg tccaagctga
     7261 agctgcgccc ccacccgagt gacagccccg cgcccgcgaa cgagcgcccg cctccgctcg
     7321 gaggtacgtc gaacagcatc acgccgcgca cgcccgggtg aaccttcgcc tcattttgca
     7381 aggtggcggc gccgtcctcg agcgacaagg tcacgtagac ggtcgagcgt gcgacgcggt
     7441 cgcgcatcca ctcgaggtcg ccgcgcacgc gctcgagcga ggcgcggacg cgctggagct
     7501 ccgcctcgat ggcgagcgct tcggtcacgt tcgcggcttt ggcgagcaac tcttgatagc
     7561 gctcgagcgc ccgctcgaga ttcgagagca ggatctccgc gtcgttgacg gtccgactca
     7621 cgtcgtgggt ctcgagcttc tgcgagcgga tcttcccgag ccgaccgagg tcggccacga
     7681 acgcctgcac ggtgttggac ggcacgcgga tggagagtga agctccccgg gtgctctgct
     7741 gatcttccac cacttcgttc acgacctggc cgttggcggc tccgaccagg cgctggagct
     7801 ctgcc
//
LOCUS       DASTKS010000326         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_721187, whole genome shotgun sequence.
ACCESSION   DASTKS010000326 DASTKS010000000
VERSION     DASTKS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721187"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFQ35_06820"
     CDS             complement(<1..331)
                     /locus_tag="VFQ35_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930378.1"
                     /translation="MFEGDARLGVLLAQASERETSSSALRFAQVAARLNEDTGFRASL
                     REASTPTRVLLTAAACASVALATFVLKPRQSWPTGDTLGVAGLLAAIFGGAIVCVRLA
                     LQPLWKAR"
     gene            complement(417..1013)
                     /locus_tag="VFQ35_06825"
     CDS             complement(417..1013)
                     /locus_tag="VFQ35_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9930379.1"
                     /translation="MLAHSSGDPAAFAELFRRYAPLLERVLLSQPLVRSEVEDIIQQT
                     FLQVHRARRDFDATQSFRPWLFTIAFNLRRVYFRRRKRRPEAVLDEEAPELARASADH
                     ARSENLHTLDRALANLSTEQREVIALHWFGGLNFSEVANALGASTAAVKVRAHRGYLS
                     LRRFLEDDGPRSENRARTVLPTPGEGAPASPRGRHGLR"
     gene            complement(1081..1611)
                     /locus_tag="VFQ35_06830"
     CDS             complement(1081..1611)
                     /locus_tag="VFQ35_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9930380.1"
                     /translation="MQHPDYKGRAALLAAAAGLCLATTSCGSDGDGVGFETGPLMRPG
                     DDCSRCHAEGSPYPSAPRWSLAGTVFPKPDSPANEGVAGVTVTVTDDAGALVTTLISN
                     EVGNFYTAAPLPKPFRVALEYQGKRIEMPCPPPAGNCGACHSLPPIGGPLGRIAVPQG
                     LTNLTGDFDCATWSRH"
     gene            complement(1598..2365)
                     /locus_tag="VFQ35_06835"
     CDS             complement(1598..2365)
                     /locus_tag="VFQ35_06835"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9930381.1"
                     /translation="MSLGDLQRKFWECVRSSTPPTANRGFVGRPPLAAARRLGIYHYA
                     YFARQEAVLGELFPRLQAVLGVPRFRELARRFVLERPSTTPVIERIGESFAKFLPESS
                     DALDFTLVDLARLEWASVEALLAPDATHDVSPAALDRIDVDRVRWIAAPSVRLLLVRD
                     GALELWRGRTNTQLTTDAPMRSLFVVITRPSDAVVHSVMSDDAGRALSRLLAGERLGE
                     AFSEFDGGAGVDRAGAALAEFLRLKLFAGWEPIDATP"
     gene            complement(2352..3167)
                     /locus_tag="VFQ35_06840"
     CDS             complement(2352..3167)
                     /locus_tag="VFQ35_06840"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9930382.1"
                     /translation="MHSGYGLRVPHYQELLVRGTRASFVEAVTENFIGRGGRPQRLLE
                     RMRRDANVALHGVSLSIGSADPIDWSYVAAVDELARQCDAVLVSDHLCFTSIEGRHAH
                     DLWPLPYTEEALALVVERARAVAERLARPFLLENISSYVEYEASAMSEWEFLAEVANR
                     ADVGILLDVNNVVVTAKNHGFSAQAYVDSLPRESVRQMHLAGHTDHGTHAIDDHASAV
                     SQEVWELYERALRRFGPVPTIVEWDDAIPPLEALEAEAAKASSLQRMISHVAG"
     gene            complement(3177..>3465)
                     /locus_tag="VFQ35_06845"
     CDS             complement(3177..>3465)
                     /locus_tag="VFQ35_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930383.1"
                     /translation="RKEASMNTNHLTRGAAIVAAAAALFAQGCSSMDDDTASNENALK
                     CLGANECKGMSECAGGTGASSCKGMNECKGMGWIYTATEAECTEKGGKVQT"
BASE COUNT          602 a         1115 c         1160 g          588 t
ORIGIN      
        1 ggcgcgcctt ccaaagcggc tggagtgcga ggcgtacgca aacgatggcg ccgccaaata
       61 tcgcggcgag cagcccagcg accccgagcg tgtcgcccgt aggccaggac tggcgcggct
      121 tgagcacgaa cgttgccaaa gcgacgctgg cgcacgctgc cgccgtcagc agtactcgtg
      181 tcggggtgct cgcttcgcgg agcgaggcgc ggaaaccagt gtcttcgttc aggcgcgctg
      241 caacttgcgc gaagcggagt gcgctgctcg acgtctcgcg ttcggaagct tgggcgagta
      301 gtaccccgag ccgtgcgtcg ccctcgaaca aggcgcggca ggcagcgcat tccgccaaat
      361 gtcgttccac ctcagcaccg gacggaagtg cgccgcccga aaagccactg cgaacgttag
      421 cgcaatccat gtcgacctcg tgggctcgcc ggcgccccct cgccaggcgt cggcaaaacg
      481 gtgcgcgctc tgttttcgct tcgcgggccg tcgtcctcga ggaaccgtcg aaggctgagg
      541 tagccacggt gcgcgcgcac cttcaccgcg gccgtgctcg cgccgagggc gttcgccacc
      601 tcggaaaagt tcaagccgcc gaaccagtgc aaggctatga cttcgcgctg ttcggtcgac
      661 aaattcgcga gcgcgcggtc gagagtgtgg aggttctccg aacgagcgtg atcggcactg
      721 gcgcgcgcga gctctggagc ttcctcgtcg agcacggcct ctggccggcg cttgcggcgg
      781 cggaagtaaa cacgccgtag attgaacgcg atggtgaaga gccaggggcg aaagctttgg
      841 gtagcgtcga agtctcgcct cgctcggtga acctgcagga acgtttgttg gattatgtct
      901 tcgacctcgg agcgcaccaa aggttgcgag aggaggacgc gctcgaggag cggcgcatag
      961 cgacgaaaaa gctccgcgaa cgccgcgggg tcacccgacg agtgggcgag catcagtgac
     1021 tcgtcgcttt gaaggggcag cgaatcgtcg aggccgcgcg aacctagctc gcttgcaccg
     1081 tcaatgacgc gaccaggttg cgcagtcaaa gtcgcccgtc agattcgtta gtccctgggg
     1141 aaccgcgatg cgcccaagag ggccgccgat cggtggtagg gagtggcacg ccccacaatt
     1201 gccggcgggt ggggggcagg gcatctcgat gcgcttgcct tggtattcga gcgcgacgcg
     1261 aaaaggcttc ggtagaggtg cggcggtata gaagttgccg acttcgttgc tgatgagcgt
     1321 cgtgacgagc gctcccgcgt cgtcagtcac ggtgaccgtc acgcccgcga cgccttcgtt
     1381 tgccggcgag tcgggtttcg gaaagaccgt tcccgcgagc gaccaacggg gcgcgctcgg
     1441 atacggtgag ccttctgcgt gacagcgcga gcagtcgtct cccggacgca tcagagggcc
     1501 ggtctcgaac ccgacgccgt cgccatccga accgcagctg gtcgtcgcca agcacaagcc
     1561 ggcggccgcg gccaatagag ccgctcgccc tttgtagtca gggtgttgca tcgattggct
     1621 cccatccggc aaaaagcttc agtcgcagaa attcggcgag cgccgcgccc gcacggtcga
     1681 cgcctgcgcc gccgtcgaac tcgctgaatg cctcgccgag ccgctcacct gcaagcaatc
     1741 gcgaaagtgc gcgtccggcg tcgtccgaca tcaccgaatg caccaccgcg tcgcttggcc
     1801 gcgtgatgac gacgaagagc gatcgcatcg gcgcgtcggt agtgagctgc gtgttggtcc
     1861 ggcctcgcca cagctcgagt gcgccgtcgc ggacgaggag gagccgcacg ctcggggccg
     1921 ctatccagcg cacgcggtcg acgtcgattc ggtcgagcgc cgcgggtgag acgtcgtggg
     1981 ttgcgtccgg ggcgagcagc gcctcgacgg atgcccactc gaggcgcgcc aggtcgacga
     2041 gtgtgaaatc gagtgcgtct gacgactcgg gcaagaactt cgcgaagctt tcgccgatcc
     2101 gctcgatgac gggcgtggtc gaggggcgct cgagcacgaa tcgtcgcgcg agctcgcgaa
     2161 accgcgggac cccgagcacg gcctgaaggc gtggaaacaa ttcgcccagc accgcctctt
     2221 gtcgcgcgaa ataggcgtag tggtagatgc cgaggcggcg cgcggctgcg agcggcggac
     2281 gacccacgaa ccctcggttg gcggtgggcg gcgtcgagct gcgaacgcat tcccagaact
     2341 tgcgctggag atcacccagc gacatgagaa atcatccttt gcagtgagct cgccttcgcc
     2401 gcttccgcct ccaaggcttc gaggggcgga atcgcgtcgt cccactcgac gatcgtcggc
     2461 accggcccaa agcgacgaag cgcgcgctcg tagagctccc aaacctcttg cgagaccgcc
     2521 gaagcgtggt cgtcgatcgc gtgcgtgccg tggtcggtgt ggccagcgag atgcatttgc
     2581 cgaacgctct ctctgggcag cgaatcgacg taggcctgcg cggagaagcc gtgattcttt
     2641 gccgtcacta cgacgttgtt gacgtcgagc aagatgccga cgtcggctcg gtttgcgacc
     2701 tcggccagga actcccattc cgacatcgcg ctcgcttcgt actcgacgta gctcgaaatg
     2761 ttctcgagca agaagggacg cgcgaggcgc tcggcgacgg cgcgggcgcg ctccacgacg
     2821 agcgccaatg cctcctccgt gtacggtagt ggccaaagat cgtgcgcgtg acgcccctcg
     2881 atgctcgtga agcacaggtg gtcggaaacg agcacggcgt cgcactggcg ggcgagttca
     2941 tcgaccgccg ccacgtaact ccaatcgatt ggatccgcgg agccgatcga aagtgagacg
     3001 ccgtgcagag cgacgttcgc atcgcgacgc attcgctcga gcaagcgctg cggcctgccg
     3061 ccacggccaa tgaagttctc cgtcaccgct tcgacgaacg atgcgcgcgt gccgcgcacg
     3121 aggagctcct gatagtgcgg cacgcgaagc ccgtagcccg agtgcatgac tgctcctcag
     3181 gtttgaactt tgccgccctt ttcggtgcac tctgcttcgg ttgccgtgta gatccagccc
     3241 attcccttac actcgttcat ccccttgcag ctgctcgcac ccgtgccgcc cgcgcattca
     3301 ctcatgccct tgcactcgtt ggcgccgagg catttgagcg cgttctcgtt cgacgccgtg
     3361 tcgtcgtcca tcgagctaca accttgggcg aagagcgcag cggcggcggc cacgatcgcg
     3421 gccccgcggg tgaggtgatt ggtgttcatc gacgcttcct tccgg
//
LOCUS       DASTKS010000327         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_499723, whole genome shotgun sequence.
ACCESSION   DASTKS010000327 DASTKS010000000
VERSION     DASTKS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499723"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFQ35_06850"
     CDS             complement(<1..680)
                     /locus_tag="VFQ35_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9930384.1"
                     /translation="MPDRPFISSFVPSSHPESAVSTPPVAELLERAIARTDEGIALYD
                     ASADPSLVYANASFYDLLAATRSALDARPLQRAFDDAYVSRALRAAVARHEGCTVHAN
                     VCADSDAARELVFSLSPIRDDDGQLTHWLCTARPLASERHSATARSEHERLSAVTLVA
                     AGLAHEINNPLASITTNLEWLVTSLGSAHPGRTTPRPSELPALSAALVDALAGAERIE
                     AAVRQLQAL"
     gene            complement(926..2665)
                     /locus_tag="VFQ35_06855"
     CDS             complement(926..2665)
                     /locus_tag="VFQ35_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9930385.1"
                     /translation="MIRRALLALCLVSGCAKPNTLPSVFTTDWLNDSGHSIAQVEQSL
                     RSAPKPTNEPVVVGLTETALVGMPLRGGAKWSRSAVPDTLPSVAGELVVYSSRGKLNA
                     LDAKTGAPRWSVDVGDFWLRGAGDDGKTTVATLGTSDRKKSLLLGVRHDGSVALRLET
                     PLELGRPAARGGVAFVPWAGQYVSAIDIASGDERGRLLTRELVSHALTVDGELFFGEK
                     AMLHFDDRVRYASTNQGARANLPDRVLPGRPKWLGSGSELPVVDVGAHARVRIYASPV
                     WSNSETRFASNSFVATYFKAVMGLNAKTGELSWVRAVSSPVIGGAPARSGFVLCGADG
                     KALFVSSRGSTLSSSDLGSPLRACVVEASSLDGGAGDDPGSLAEQIDRAFSTLDPEMA
                     AAEAFLVTELGRLPDAGVTKTLIDLSTSARMPPDIRLRARELLAQRRSGADLMLAALE
                     HRYDFLSGELLPPPVGPLADALAAMGEKRAAPALARHLNDPSTDSGDLVRAARALMTL
                     ATPSELPGLRTFFALYRATADEPALIQAVGLVARAIVRVGGTEGRALVERATKDPLTQ
                     PDVVKELATVLPS"
     gene            complement(2662..>3458)
                     /locus_tag="VFQ35_06860"
     CDS             complement(2662..>3458)
                     /locus_tag="VFQ35_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930386.1"
                     /translation="AAPTAGKAEKPKPRAEKPPKLDLDRYRRTLETGTESAILDALSE
                     LGALGKGGAAAAPLVDALLARGANGTVVLKALETEGELGVAASSDAVAPYVAHRRPEI
                     RQAAATVLASTGGPSAVAALRRALAGPDPAVRGVAAAGLGTLGAKEAVEDLFTVLSHD
                     TPQAALSIATLCNPEQCDRLMALVGKLKFDVLEPAFVPLLLRPASELPDANKLRYVER
                     LRVLATRPAAAVLQTALNKLPKDESPVLRNALQNALKARPIVGDTK"
BASE COUNT          545 a         1132 c         1234 g          547 t
ORIGIN      
        1 agcgcttgca gctggcgtac ggcggcttcg atgcgttcgg cgcctgcgag cgcgtcgacg
       61 agcgccgccg agagggcagg gagctcgctg gggcgcggcg tcgttcgccc cgggtgcgcg
      121 ctgccgagcg acgtaacgag ccactcgagg ttggtcgtga tcgaagcgag cggattgttg
      181 atctcgtgcg cgagccccgc cgcgaccagc gtgaccgcgg agagcctctc gtgttcgctg
      241 cgtgccgtcg cgctgtgccg ttcgctcgcg agcgggcgcg cagtgcaaag ccagtgagtg
      301 agctgaccgt cgtcgtcgcg gatcggtgag agcgagaaaa ctagctcgcg agccgcgtcg
      361 gagtccgcgc acacgttcgc gtggacggtg cagccctcgt gtcgagcaac cgccgcgcgc
      421 agggcgcggg agacgtaggc atcgtcgaac gcgcgctgta gggggcgagc gtcgagggcg
      481 ctccgcgtcg ccgcgagcag gtcgtagaac gatgcattcg cgtaaacgag cgagggatcc
      541 gcgctcgcgt cgtagagcgc gatgccctcg tcggttcgcg cgatggcgcg ctcgagcagc
      601 tccgcgactg gtggcgtcga aacagcgctc tcggggtgcg agctaggaac gaatgaggag
      661 atgaatgggc gatccggcaa ctgtcgctcg agttcgcccc ctcacgaatc gttatagccc
      721 tttttgtacg gcacggtagt gaccgcggtc gcgcacgcgt tgctgtcgtc gattcgagcg
      781 ctcgtacgtc gcgcgccgat tcacccttca cagtgaaatt ctgccgcgag tgttcgcgaa
      841 cgaggtcgcg tgcgcgagac cagttctgcc aacgcgatga gcgtgacggg gcgctgctgg
      901 cgtgcggctc cagttggcgg agcgctcagc ttggcaggac ggtcgcgagc tctttcacga
      961 cgtctggctg agtcagcgga tctttggtcg cgcgctctac gagtgcgcgt ccttcggtgc
     1021 caccaacgcg cacgatggcg cgagccacca agccgaccgc ctggatcagt gcgggctcgt
     1081 cggccgtcgc gcgatacagc gcgaaaaagg tccgcagccc ggggagctcg gagggcgtgg
     1141 ccagcgtcat gagggcgcga gcagcgcgca ccaaatcgcc tgagtcagtg gagggatcat
     1201 tcaaatgacg cgcgagcgct ggcgcggcgc gcttttcgcc catcgcggcc agcgcgtcgg
     1261 cgagcggacc aacgggcggg ggaaggagct cccccgaaag gaaatcgtag cgatgctcga
     1321 gcgcggccaa catcaagtcg gcacccgagc gccgttgggc gaggagctcg cgggcacgca
     1381 ggcggatgtc gggaggcatg cgagcgctcg tgctgaggtc gatgagggtt ttggtcacgc
     1441 ccgcgtcggg gagccggccg agctcggtga cgaggaaggc ctcggccgcg gccatttccg
     1501 gatccagggt gctgaaggcg cgatctatct gctccgcgag gctacctgga tcgtcgccgg
     1561 cgccgccgtc gagcgagctc gcttcgacga cgcaagcgcg caacggtgag ccgagatccg
     1621 agctcgacag agtcgagccg cggctgctca cgaagagcgc ctttccgtcg gcgccgcaca
     1681 acacgaaccc ggagcgcgcc ggtgcaccgc caatgaccgg gctcgatacc gcgcggaccc
     1741 agctgagctc gccggtcttg gcgttgaggc ccatcacggc cttgaagtac gtcgcgacga
     1801 agctgttcga ggcaaagcgc gtctccgagt tggaccacac cggggaagcg tagatgcgca
     1861 ctctagcgtg cgcgccgacg tcgacgacgg gcagctccga acccgaaccg agccacttgg
     1921 ggcggccggg tagcacgcga tcgggcaggt tcgcgcgcgc gccctggttg gtgctcgcgt
     1981 agcgcacgcg atcgtcgaag tggagcatcg ccttctcgcc gaagaagagc tctccgtcga
     2041 cggtgagagc atgactgacg agctcgcggg tcaagagccg cccgcgctcg tcaccgctcg
     2101 cgatgtcgat cgcgctcacg tattgacctg cccacggcac gaacgcgacg ccaccacgtg
     2161 ctgccgggcg gccgagctcg agcggcgttt cgaggcgcag cgcaaccgag ccgtcgtgcc
     2221 gaactccgag gagcagactc tttttgcggt cactcgtgcc gagcgtggcg acggtggtct
     2281 ttccgtcgtc gccggcgccg cgaagccaga aatcgccgac gtcgacgctc cagcggggag
     2341 cgccggtctt cgcgtcgagc gcgttcagct tgcctctcga ggagtagacg acgagctcgc
     2401 cggcgacgct cggcagcgtg tccggtacgg ccgaacgact ccacttcgct cctccgcgca
     2461 gcggcattcc cacgagcgcg gtctcggtga gccccacgac cacgggctcg ttggttggct
     2521 ttggcgccga gcgcagcgac tgctcgacct gagcgatcga gtgaccgctg tcgttcaacc
     2581 aatccgtcgt gaaaacggac ggcaaagtgt tcggtttggc gcagccgctg acgaggcaga
     2641 gcgcgagcag ggcccggcgg atcatttggt gtctcccacg atgggtcgcg ctttcaacgc
     2701 gttctgaagg gcgttgcgca ggacggggct ctcgtctttg ggcagcttgt tcagcgcggt
     2761 ctgcaagacc gctgccgccg ggcgcgtcgc gagcactcgc agccgctcga cgtagcggag
     2821 cttgttggcg tctgggagtt cactcgccgg gcgcagcaag agcggcacga aggccggctc
     2881 gagaacgtcg aacttgagct ttccgaccag cgccatcaaa cgatcgcact gctccggatt
     2941 gcacaacgtg gcgatggaga gcgcagcttg cggcgtgtca tgcgaaagaa ccgtgaagag
     3001 gtcctccacc gcctctttgg cgccgagcgt gccgagcccg gccgccgcta ctccccgcac
     3061 cgccggatca ggcccggcga gcgcgcgtcg cagcgccgcc accgccgagg gcccaccggt
     3121 cgaggccagc actgtcgcgg ccgcctggcg gatctccggc cttcggtgcg ccacgtaggg
     3181 cgctaccgcg tcgctcgacg ccgcgacccc gagctcgccc tcggtctcga gcgccttcaa
     3241 gacgacggtg ccatttgcgc cgcgagcgag cagcgcgtcc accaagggcg cggccgctgc
     3301 accgcctttg ccgagcgcgc cgagctccga caaggcatcg aggatcgcag actcggtacc
     3361 cgtctccaac gttcgacggt agcggtcgag gtcgagcttg ggcggctttt cggcccgggg
     3421 tttcggtttt tccgcctttc cggcggtcgg agcggcga
//
LOCUS       DASTKS010000328         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_54130,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000328 DASTKS010000000
VERSION     DASTKS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54130"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1049)
                     /gene="fdhD"
                     /locus_tag="VFQ35_06865"
     CDS             complement(213..1049)
                     /gene="fdhD"
                     /locus_tag="VFQ35_06865"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET9930387.1"
                     /translation="MNLPSDRATRAGMRRAPIARVRSDQLERVDDVLAVEEPLEVRIG
                     DETWATLMRTPGSDHELVVGLLLAEGIIRSALDVVRVAHCERLDPERTHNVIEVDLAA
                     DAGRAELAERRTVSSSACGVCGRTSIDDLLERVERPSTTPNFDAAWLAALPEMLARAQ
                     RNFVHTGGLHAAAIAHPERGVLALREDIGRHNAVDKAVGRLLLDRALPAHDSALVVSG
                     RAGFEIAQKAVVAGLGALVSVSAPSSLAVETAVRSGLVLLGFARGGDYNVYSAFERLR
                     GI"
     gene            complement(1046..3268)
                     /locus_tag="VFQ35_06870"
     CDS             complement(1046..3268)
                     /locus_tag="VFQ35_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HET9930388.1"
                     /translation="MRALFFKWLDWLRVFVPFGVLWRDKPRHYREILKIVWENRGRWG
                     YALRILRHGVCDGCSLGPRGLRDDVVPGVHLCLTRLKLLRLNTMGPIPDEQLRDVSAL
                     SRLSNEELHALGRVPYPLIRRRGDKGFSKISWDEALELAASRWRSASPDDVGVFLTSR
                     GVTNETYYVAQKLARIAGTPHVDTCARLCHAASTVGLKETLGFAAPTLSLNDWIGTDL
                     LFIVGSNLPNNQPVSTKYLYYARKAGTRVIVVNPFREPALERYWIPSIADSAVFGTRL
                     ADEFHLVRPGGDIAFFMGVLKALDAIGGWDERFVAERTSGADELRAELRRMSWQEIVS
                     DSGIEQGELERLARLYAKAKTAVVVYSMGLTQYEFGVDNVKMVVNFALARGMIGRKNC
                     GIMPIRGHSGVQGSAECGADPEKLPGGAPLDAANIARFEAMWKHPIPKSPGLRAAHLL
                     DRAGDRGLDVLYLVGGNHLETMPDRIHARRALERVKLRIHQDIVLNSSTLLEAEEAVL
                     VLPAQTRYEQKSGGCSTSTERRIRFTPEIPGPRIAEAKAEWEIPALIGQRMTPDRKDL
                     FDYADTRAIRTEMAALMPLYRGIERLEREGDAVQWGGPQLGSEIFPTADGKAHFTKVS
                     IPRIDVPVDHFLLTSRRGKQFNSMTYGKRDPLMGGAERNAVLVDSRDIEQLGLREGDP
                     VRVVAEHGEMQATLQSGPCRRGHVQAYWPECNVLVGRRYDKASGEPDYNTVVRIERAQ
                     "
     gene            complement(3831..4223)
                     /locus_tag="VFQ35_06875"
     CDS             complement(3831..4223)
                     /locus_tag="VFQ35_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930389.1"
                     /translation="MANSRVAWFSRVRTSSRVQAEPFRSRHFEGNLLAELRVNAEAEA
                     AYRALVMDTRFEAGTLLVETLLGRDGAPAALLALERRAEGWAFSEVDPEGVPLPFDES
                     LCRGCHAGALAAPVFGPARIVPERESKR"
     gene            4328..>4740
                     /locus_tag="VFQ35_06880"
     CDS             4328..>4740
                     /locus_tag="VFQ35_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9930390.1"
                     /translation="MRCAGAHGTTAWVRFVASLPLIAMVGFFACARPTPAAVASAAPP
                     PKSSAAPVASERAPTASDEADVPEAASDPDAPAWLGVELALRMPEDPGVLVRSVVPGS
                     PAERAGVLAGDVILSVEGQTVGRPSELVAEIAGRH"
BASE COUNT          780 a         1556 c         1581 g          823 t
ORIGIN      
        1 agcgaagctc gagccactcg cggggccgcg gagcgccatc gaagcgctgc agcatcttcg
       61 gttcgaggtc cacgtgcgct tcatcgagct ccttgaggag cgccatcacg gcgtcgattt
      121 cggcgaaccg cgcacttcgg atcgtgagcg ccaccgcatg tatatgccac gtcacgcgcg
      181 cggcgccgtt tcagttctcg cgtagcagcg tttcagatgc cgcgaagtcg ctcgaaggcg
      241 gagtacacgt tgtagtcgcc gccgcgcgca aaaccgagca gcacgagccc cgaacgcacg
      301 gccgtttcga ctgcgagcga gctcggcgcc gacaccgaaa ccagcgcgcc gagcccggca
      361 acgacggctt tttgcgcgat ttcgaagccg gcgcggccgc tgacgacgag cgcggagtcg
      421 tgcgctggta gcgcgcggtc caagagcaac ctgcctactg ccttgtcaac ggcgttgtgc
      481 cgcccaatgt cttcgcgcaa cgcgagcacg ccgcgctccg gatgcgcgat cgccgctgcg
      541 tgcagcccac cggtgtggac gaaattgcgt tgcgcccgcg cgagcatttc cggcagcgcc
      601 gcgagccagg ccgcgtcgaa gttcggcgtg gtcgacgggc gctcgacgcg ctcaagtaag
      661 tcgtcgatgc tggttcggcc gcacacgccg cacgcggagg aagatacggt gcggcgctcg
      721 gcgagctcgg cccgtcccgc gtccgccgcg aggtccactt cgatcacgtt gtgcgtccgc
      781 tcggggtcga gccgttcgca atgcgcgact cgcacgacgt cgagcgcaga gcggatgatg
      841 ccctcggcga gcagcaagcc gacgacgagc tcgtgatccg agcccggcgt ccgcatcaac
      901 gttgcccaag tttcgtcgcc gatgcgcact tcgagcggct cttccaccgc cagcacgtcg
      961 tccacacgtt cgagttggtc ggagcgcacc cgcgcgatgg gcgcgcgccg catgccggcg
     1021 cgggtcgcgc gatcgctcgg taggttcatt gcgcgcgctc gatgcggacc accgtattgt
     1081 agtcgggctc gcccgaagct ttgtcgtagc ggcgcccgac gagcacgttg cactcgggcc
     1141 aataggcttg cacgtgtccg cgtcggcacg ggccgctctg caacgtggcc tgcatttcgc
     1201 cgtgttcggc cacgacgcgc acggggtctc cctcgcgcaa gccgagctgt tcgatgtcgc
     1261 gcgagtcgac caaaacggcg ttgcgctcgg cgccgcccat gagcgggtcg cgcttgccgt
     1321 acgtcatcga gttgaactgc tttccacgtc ggctcgtgag caagaagtgg tcgaccggca
     1381 cgtcgatgcg cgggatgctc accttggtga agtgcgcttt gccgtcggcg gtggggaaga
     1441 tttcgctgcc gagctgcggc ccgccccatt gaacggcgtc gccttcgcgc tcgagccgct
     1501 cgatcccgcg gtagagcggc atcaatgcgg ccatttcggt tcggatcgcg cgggtgtcgg
     1561 cgtagtcgaa gaggtccttt cggtcgggcg tcatccgctg accgatgagg gcagggatct
     1621 cccactcggc tttggcttcc gcgatgcgcg ggcccgggat ctccggggtg aagcggatgc
     1681 gtcgctcggt cgacgtcgag cagccaccgc tcttttgctc gtagcgggtc tgcgcgggga
     1741 ggaccagcac cgcttcttcg gcctcgagta gcgtcgagct attcagcacg atgtcctggt
     1801 ggatgcggag cttcacgcgc tcgagcgcac gacgcgcgtg gatgcgatcc ggcatcgtct
     1861 ccaggtgatt acccccaacc aaatacagga cgtccaaacc gcgatcgccg gcgcgatcca
     1921 agagatgcgc agcgcggagc ccgggtgact tcgggatggg atgcttccac atcgcttcga
     1981 agcgcgcgat gttggcggcg tcgagcggcg cgccaccggg gagcttttct ggatctgcgc
     2041 cgcactcggc cgagccctga acgcccgaat gcccgcgaat gggcatgatg ccgcagtttt
     2101 ttcggccgat catgccgcgc gcgagcgcga agttgacgac catcttcacg ttgtcgacgc
     2161 cgaactcgta ctgcgtaagc cccatcgagt agacgacgac cgccgtcttc gctttggcgt
     2221 agaggcgcgc gaggcgttcg agctcgcctt gctcgatgcc agagtccgat acgatctctt
     2281 gccagctcat gcgcctgagc tcggcgcgta gctcgtcggc gccgctcgtg cgctcggcga
     2341 cgaagcgctc gtcccaaccg ccgatcgcgt cgagcgcctt cagcacgccc atgaagaagg
     2401 cgatgtcacc gcccgggcgc accagatgaa actcgtcggc gagccgcgtg ccgaacaccg
     2461 ccgagtcggc gatgctcggg atccaataac gctcgagcgc cggttcgcga aacgggttca
     2521 ccacgatgac gcgagtcccg gctttgcgcg cgtagtacag gtacttggtc gaaacgggct
     2581 gattgttggg caggttgctg ccgacgatga acaggaggtc ggttccgatc cagtcgttca
     2641 gcgagagcgt gggcgccgcg aagccgagag tctctttcaa gccgacggtc gaggcggcgt
     2701 ggcacaagcg agcgcacgta tcgacgtgtg gcgtaccggc gatgcgcgcg agcttttgcg
     2761 cgacgtagta agtctcgttc gtgacgccac ggctggtgag aaagacgccg acgtcgtcgg
     2821 gcgatgcgga gcgccagcgc gaagcggcga gctcgagcgc ctcgtcccag ctgatcttcg
     2881 agaagccttt gtcgccgcgt cggcggatca gcgggtacgg cacgcggccg agcgcgtgta
     2941 gctcttcgtt gctgagccgc gagagtgcgc tcacgtcgcg cagctgctcg tcgggaatgg
     3001 gtcccatcgt gttcagccgc aacagcttca ggcgagtcag gcagaggtga acgccgggga
     3061 cgacgtcgtc acgcagtcca cgcggaccga gggagcatcc gtcgcaaacg ccgtggcgca
     3121 agatgcgcag cgcgtagccc caacgtccgc gattctccca aacgatcttc aaaatttccc
     3181 ggtagtgccg tggcttgtcg cgccaaagca ccccgaacgg tacgaacacc cgcagccagt
     3241 cgagccactt gaaaaacaac gcacgcacga agcgcagctt agcctccgta cgagtcgtga
     3301 tgttttcgtc agtcagcgtg tcgttcggcc ggtcaaccgc cggtttccag tcgacagctg
     3361 aggcgatgcg cctcgcctga cttttccgcc ccgatggcag ctcgcggcca tgcctggaaa
     3421 agtttgcggg gctctatggg gggcatgaca gggcgaccat ggatctggcc tgcgggacta
     3481 aatcgaattg gtcagagcag ttaaaaatat caataaattc aagctgttca taatcaaggt
     3541 cggcgctggc acgactggtg cttaagggga cagcgccgag gccaacaccc tcggcgcctt
     3601 ccttgtcttg ttcccttggc caacgttggc ccattcgctt gagcttgaaa gtctcgcttc
     3661 cttagtcgct ttagctttcc ctccttagct tcacctcctt actttcgaga gcgagcagaa
     3721 ttcgtctcca aggcccgaca cccaaccggt tcgggctttg gcgctttgtg ggggtcactt
     3781 cggcggagga gccacggtgt cgaaccgcgc tccgttctgc aacgcggcgt tcagcgtttc
     3841 gattcccgct ccggaactat ccgcgccggc ccgaatacgg gagctgcgag cgctccggca
     3901 tggcagccgc ggcaaagtga ttcatcgaaa gggagtggaa ccccctcggg atcgacctcg
     3961 ctgaatgccc agccctcggc gcgccgctcg agcgcgagca aagctgctgg tgcgccgtcg
     4021 cgaccgagca gcgtctcgac gagcagggtg cccgcctcga agcgcgtgtc catgaccagc
     4081 gcgcgatacg cggcttcggc ctccgcgttg acgcggagct cggcgagcag gttgccctcg
     4141 aagtgccgtg aacgaaacgg ctccgcttgg acgcgcgaag atgttcgtac gcgcgagaac
     4201 caagcgacgc gcgaattcgc cacgctcggg gggtgagcgg cgggagtgtg cgacgcactc
     4261 gcacgctgaa ccacgggcgc gcgcgcttcg cgagtcgaac acgcgagcac gctgaagagc
     4321 agggcaagtg cgatgcgcag gcgcgcatgg taccactgcc tgggtgcgct tcgtagcttc
     4381 gctgccgctg atcgcgatgg tcggcttctt cgcctgcgca cgaccgacac cagcggccgt
     4441 ggcgagcgcc gccccaccgc cgaagagcag cgcggcacca gtcgcgtccg agcgcgctcc
     4501 cactgcgtcc gacgaagccg acgtccccga ggccgcgagc gatcccgatg cccccgcatg
     4561 gctcggtgtc gagctcgcac tgcgcatgcc ggaggacccc ggtgtgctcg tgcgctcggt
     4621 cgttcccggc tctcctgccg agcgcgcggg cgtgctggcg ggggacgtga tcctcagcgt
     4681 cgaagggcag acggtggggc gaccttccga gctcgtcgcc gagatcgcgg ggcgacacca
//
LOCUS       DASTKS010000329         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_721924, whole genome shotgun sequence.
ACCESSION   DASTKS010000329 DASTKS010000000
VERSION     DASTKS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721924"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFQ35_06885"
     CDS             complement(<1..453)
                     /locus_tag="VFQ35_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930391.1"
                     /translation="MARKGRPPPAASEPTLLSRAAEHGSLGGPPKPLADRLRPRSLDE
                     VVGQLHLLSEGKLLGDAIRKDRVPSLILWGPPGSGKTTLAHVIAHVTRTEFDSLSAVL
                     GGVAELRAIIDQARERRRYQGRGTTLFVDEIHRFNKSQQDAFLPHIEDG"
     gene            complement(456..1181)
                     /locus_tag="VFQ35_06890"
     CDS             complement(456..1181)
                     /locus_tag="VFQ35_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9930392.1"
                     /translation="MSVKDELSKVRARIREATLAAGREANEVELIAVSKLQPSAAIRE
                     AYAAGQRDFGENYVQELCEKAAELRDLSEIRWHLIGHLQRNKAKKIAPLITAIHSVDS
                     VELVLELGKRVAAAEAERRALLGAERARLDVLVEVSIAREAQKSGAAPEDVAKVIEAI
                     EREPSLRLRGLMCVPPMSEQPEDARPHFEALVRLRDTLGGRAHLPELSMGMTADLEVA
                     IAAGATSVRVGTAIFGQRPRPGT"
     gene            complement(1194..2279)
                     /locus_tag="VFQ35_06895"
     CDS             complement(1194..2279)
                     /locus_tag="VFQ35_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HET9930393.1"
                     /translation="MQRAPEIAAFPWSALELVDRLAESNLWTARRWLERSVEISRLAA
                     ALGSLVGSEVRIFKRSVRARSDGPSLRLGFRPQGGGAVCVLGLEPALAAALLSSLFHR
                     SVPVVGPDAVFDASAHGALSAIFIEAARASEPSLPFEPCKPDLVGNGAAVEITVVLSG
                     KPYAAVVWVSTQRYTVPLADDASLLGSLGEFTIGLPLVVALASTTPRALAELAIGAAF
                     CAGTETWIDARGAGRGVLVSAQSERGLGVELGPEGRIVLREPARIPLTSAEEPSMSTD
                     DSGPPTLAQAVLDAPVVVRVELGSVSMSAREWAALRPGDVLQTGHRLGEPVLLRAGGR
                     VVARGELVNVEGEVGVRVLEVGSDANA"
     gene            2454..>2816
                     /locus_tag="VFQ35_06900"
     CDS             2454..>2816
                     /locus_tag="VFQ35_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930394.1"
                     /translation="MSKIPRVCAVQTKPVWWNVSPALRKSLLAFALTACSAGVVGCGS
                     NQSQGRDQLVVVSERLGGSWRVQTFTPEQQLELPLQSLLNAELGQLIITFGNGGYTAK
                     GPGIDIAGRFQLTGGGGDL"
BASE COUNT          469 a          950 c          937 g          460 t
ORIGIN      
        1 gccgtcctcg atgtgcggca agaaggcatc ttgctggctc ttgttgaagc gatggatctc
       61 gtcgacgaac agagtcgtgc cccggccctg gtaacgacgc cgttcccgcg cctgatcgat
      121 gatcgccctg agctcggcga caccgccgag aacggcgctc agcgaatcga actcggtgcg
      181 ggtcacgtgc gcgatgacgt gcgcgagcgt cgtttttccg gagccgggcg gcccccacaa
      241 gatgagtgac ggcactcgat ctttgcggat cgcatcgccg agcaacttcc cttcggagag
      301 caggtgcagc tgtccgacga cctcgtcgag tgagcgcggg cgcaaccgat ccgcgagcgg
      361 cttgggcgga ccgcccagcg agccgtgctc ggcggcgcgg ctcaagagag tcggctcgct
      421 cgcggcagga ggagggcggc ccttgcgtgc catgttcacg tgccggggcg cggtcgctgc
      481 ccgaaaatcg cggtgccaac gcgcaccgaa gtagcacctg ccgcgatggc tacctcgaga
      541 tcggcggtca tacccatgct gagctcgggc aagtgagcgc gcccgcccaa ggtgtcgcgc
      601 aagcgaacga gcgcctcgaa gtggggacga gcgtcttcgg gttgctcgct catcggcggc
      661 acgcacatga ggccgcgcag acgcagcgaa ggttcgcgct cgatggcttc gatcactttg
      721 gctacgtctt ccggcgcggc gccgctcttc tgggcttccc gagcgatgct cacttcgacg
      781 agcacgtcga gtcgcgcgcg ctcggcgccg agcagagcgc ggcgctcggc ctccgcggct
      841 gcgacgcgtt tgccgagctc gagcacgagc tcgactgaat cgacgctgtg aatggcggtg
      901 atgagaggcg cgatcttctt cgccttgttc cgttgcaaat gaccgatgag gtgccagcgg
      961 atctcgctca gatcgcgcag ctcggccgcc ttttcgcaga gctcttggac gtagttctcg
     1021 ccgaaatctc gttgtccggc ggcgtacgcc tcgcgaatgg cggcgctcgg ctgtagcttc
     1081 gaaaccgcga tgagctcgac ttcgttcgct tcgcgacccg cggcgagcgt cgcttctcgg
     1141 atgcgcgctc gcaccttcga aagttcgtcc ttcacgctca tcgttccggc acctcaggcg
     1201 ttcgcatcgc ttccgacttc gagcacgcgg acgcccacct cgccctcgac gttgacgagc
     1261 tctccgcgcg cgaccacgcg gccaccggcg cgcagcagca ccggttcgcc gaggcgatgt
     1321 cccgtttgca ggacgtcacc aggtcggagc gcagcccact cgcgcgccga catgctcacg
     1381 ctgccgagct cgactcgaac cacgacgggt gcgtcgagca ccgcttgagc caacgtaggc
     1441 ggtccggaat cgtcggtcga catgctcggc tcctcagcgg aagttagggg aatccgcgcg
     1501 ggttcccgta gcacgattcg tccttcgggt ccgagctcga cccccagtcc gcgctcgctc
     1561 tgcgcgctca cgagtacgcc gcggcccgcg ccgcgcgcat cgatccacgt ctcggtgccg
     1621 gcgcagaacg cggccccgat cgcgagctcg gcgagcgcgc gcggcgtcgt cgaggcgagc
     1681 gccacgacga gcggcagtcc aatcgtgaac tctccgagcg acccaagcag cgaagcgtcg
     1741 tccgcgagcg gcaccgtgta gcgctgggtc gagacccaaa cgacggccgc gtacggcttg
     1801 cccgaaagca cgaccgtaat ctcgacggcg gcgccgttcc cgacgaggtc aggcttgcat
     1861 ggctcgaacg gcaacgaagg ttcgctagcg cgcgctgcct cgatgaagat cgcgctgagg
     1921 gctccgtgag cggaagcatc gaacaccgcg tcgggcccaa cgactggtac gctccggtgg
     1981 aacaacgacg agagcagtgc ggccgcgagc gccggttcga ggcccagcac gcagacggcg
     2041 ccgcccccct gaggacgaaa ccctagccgg aggctcggtc catccgagcg agcgcgcacc
     2101 gaacgcttga agatgcggac ctccgaaccc acgaggctgc cgagagcggc ggctagccgc
     2161 gagatctcca ccgagcgctc gagccagcgg cgagccgtcc acaagttgct ttccgccagc
     2221 ctgtctacca gctccagggc cgaccacgga aatgccgcaa tttcgggtgc gcgctgcacg
     2281 tgccgcatcc tacatggcgc gaggttcacc ggtctgtcca caagtgaccc acaaactgtg
     2341 gataaatcct gtggactacc tggcgagact cgccagggcg aagatcatga ggcccggctg
     2401 gttggccgcg tcgacgcgct cgagcgagcg ctggagagtt cggagaaagg cgtatgtcca
     2461 agatcccgcg cgtttgcgcc gtacaaacga agcccgtatg gtggaacgtg tcgcccgcgc
     2521 ttcgtaagtc gttgctcgct ttcgcgttga ccgcctgctc cgcgggcgtc gttggctgtg
     2581 ggtcgaacca gtcacaaggg cgagatcagc tcgtcgtcgt cagcgagcga ctgggcggta
     2641 gttggcgtgt gcagaccttc accccggagc agcagctcga gctgcctctc cagagcctgc
     2701 tcaatgcgga gctcgggcaa ctcatcatca cgttcgggaa cgggggttac accgccaagg
     2761 gcccgggcat cgacatcgcg ggtcggttcc agttgaccgg cggcggcgga gatctc
//
LOCUS       DASTKS010000330         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1168852, whole genome shotgun sequence.
ACCESSION   DASTKS010000330 DASTKS010000000
VERSION     DASTKS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168852"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1414
                     /locus_tag="VFQ35_06905"
     CDS             299..1414
                     /locus_tag="VFQ35_06905"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET9930395.1"
                     /translation="MSSVSPPPTAPASSSASTTQPAPAAGIQKAAFGKHEDKDVELFT
                     LTNKNGLVLKAITYGAAVTELFVPDRTGKLGDVVVGFDTLQGYETGTGFFGATVGRVA
                     NRIRGAKFSLEGKTYALTASDPPHHLHGGAKGWDKVVWTGESVETPDGPSVIFTYVSK
                     DGEEGYPGTVTARTTYTLTNQNEFKVVMEATTDKTTLVNMAHHTYWNLGGFASGPILD
                     HELTLFADKYTPGDPVVPTGVIKQVLGTPFDFTSAKPIGRDLKDVGNKPVGYDHNWVV
                     NGDPHTMRPVARLKDPKSGRVLTLEADQPGVQFYSGNFMDGKAHGKGATYAQYTGLCL
                     ETQKFPNSINVPAWKNEVILKPGQTYRHTMIHRFSAE"
     gene            1411..2427
                     /locus_tag="VFQ35_06910"
     CDS             1411..2427
                     /locus_tag="VFQ35_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9930396.1"
                     /translation="MRTPLGWGVIGTGSIAAAFTEAVASSTRARIVSVAGSSPEKGRA
                     FARHWSVPHAATSVAELVRHEAVEAVYVASPHPRHEEHALAAIGAGKAVLCEKPLATS
                     SDSAERIVRAAKSAGVFLMEAYMYRCHPLLEHALTLLRSGAIGRIEHVRAHFGFRVER
                     NLDGRLFNPNLGGGAILDVGGYPVSFARLIAGLAEDAAFAEPVELSAQGRLGPSGVDE
                     LAVALLRFPSGVTAELGCATRHELGTAAAVFGDRGWLELPNPWIPRGARLGLESDLIV
                     HRDGHDPEHVVVTTGLPTYAIEAELVAETLPALDPRPPAMSSADTLGNMRVMEAWSRA
                     LRSV"
     gene            2467..>3438
                     /locus_tag="VFQ35_06915"
     CDS             2467..>3438
                     /locus_tag="VFQ35_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET9930397.1"
                     /translation="MSVTFKALCLTSLCALLTVVGCNKSGGASGSQPSGSAAPAQDKV
                     VKLAFVTNNASEFWKIAAAGVHKYEKEGKVQVDIKMPANGTTEEQNQILENLSSQGYD
                     AIAVSAIAPKDQVPILDRVALKTKLITFDSDAPASKRLLYIGTNNYEAGKALGNEIVK
                     LLPNGGKMAVFVGTLAADNAQQRLQGIQDVVKEHKIEIVDKREDNTDRAKARSNVEDI
                     INAHSDLKLVAGLWSYNGPAIAAAIEALGKKGKVIAAVFDEEDGTLNAIAADTISCTV
                     VQKPFQFGYLSSKWAHDLATHPDTALAAIPGNKSIDTGVEVINKANVA"
BASE COUNT          669 a         1093 c         1113 g          563 t
ORIGIN      
        1 gatccgaacg cataaaagcc gattctggag ctctcaattc tcgccgccca ggtacgagcc
       61 cagcgtggcg ctctggcgct tgccgcgggg gagcggcgct aaactgggtg ggcgaacgag
      121 gcgctgcgga gcgcctcgaa gctctggtat ggtctcgggc cgccgagcgc cgttcgagcg
      181 tcggctcgga ggactcacca tgctcactcg ccgattctgc acgtttgctt gtttggcagg
      241 cctgctgagc ggttgcgcgc cgaccacgtc agcgcccaaa gctcaagaac ccgtcgccgt
      301 gtcatcagtg tcccccccgc cgacggcccc cgcgagctcg agcgcttcaa cgactcaacc
      361 ggcgccggct gcgggaattc agaaggccgc cttcggcaag cacgaggaca aagacgtgga
      421 gctttttacc ctaaccaaca aaaacggtct cgttctcaaa gccatcacgt acggcgccgc
      481 cgtcaccgag ctgttcgtgc ccgaccgcac cggcaagctc ggcgacgtgg tcgtcgggtt
      541 cgatacgcta caaggctacg agactggaac cggctttttc ggtgcgacgg ttggccgcgt
      601 cgcgaatcga attcgtggcg cgaagttttc cttggaaggg aagacgtacg cgctgaccgc
      661 gagcgacccg ccgcatcatc tgcacggcgg cgccaagggc tgggacaagg tcgtgtggac
      721 gggagagagc gtcgaaacgc ctgacgggcc gtcggtgatt ttcacctacg tttcgaagga
      781 cggcgaagaa gggtatcccg gcaccgtcac tgcgcgcacg acgtacaccc tcaccaacca
      841 gaacgaattc aaagtcgtga tggaggcgac caccgataaa acgacgctgg tgaacatggc
      901 gcaccacacg tactggaact tgggtgggtt cgcctccggc cccatcctcg atcatgagct
      961 cacgcttttc gccgacaagt acacgcccgg cgatccagtg gtgccgaccg gcgtcatcaa
     1021 acaggtgtta ggtacgccgt tcgatttcac gagcgcgaag ccgatcggtc gcgatctgaa
     1081 ggacgtcggc aacaagcccg ttggctacga tcacaactgg gtcgtcaacg gagaccccca
     1141 tacgatgcgc cccgtcgcgc gcctgaaaga tcccaagtcg gggcgtgtgc tcacgctcga
     1201 agccgaccaa cctggggtgc agttttactc gggcaatttc atggacggaa aggcgcacgg
     1261 caagggcgcg acctacgccc agtacacggg gctttgcctc gaaacgcaaa agttcccgaa
     1321 ctcgatcaac gtaccggcat ggaagaacga ggtcatcttg aagccgggtc agacctaccg
     1381 gcacacgatg atccaccgct tcagcgcaga atgaggacgc cgctcggctg gggcgtcatc
     1441 gggacgggga gcatcgcggc ggctttcacc gaagcggttg cttcctcgac gcgggcgcgc
     1501 atcgtgagcg tcgcgggcag ctcgcccgaa aagggcaggg cgttcgcacg acactggtcg
     1561 gttccgcacg ccgccacgag cgttgcagag ctcgttcggc acgaagcggt cgaggccgtc
     1621 tacgtcgcga gcccgcatcc gcgccacgag gagcacgcac tcgccgcgat cggcgcgggc
     1681 aaggcggtgc tgtgcgaaaa gccgctcgcg acttcgagcg attcggcgga gcgcatcgtg
     1741 cgcgccgcga agagcgccgg cgtcttcttg atggaagcgt acatgtatcg ctgccatccg
     1801 ttgctcgagc acgcgctcac tctgctccgc tcgggagcga ttggtcgcat cgagcacgtg
     1861 cgtgcgcact tcgggtttcg cgtcgagcgc aacctcgatg gccgtttgtt caatccgaac
     1921 ttggggggcg gcgcgatcct cgatgtcgga ggctatccgg tctcgttcgc gcggctcatc
     1981 gcgggcctcg ccgaggacgc cgcgtttgcg gaaccggtgg agctttcggc ccagggtcga
     2041 ctcggtccga gcggagtgga cgagctcgcc gtcgcgctgc tgcgctttcc gtcgggcgtc
     2101 accgccgagc tcgggtgcgc gacgcgtcac gagttaggaa cggcggctgc cgttttcggc
     2161 gaccgaggtt ggctcgagct gccaaacccg tggatccccc gcggcgctcg ccttggcctc
     2221 gaaagcgact tgatcgtgca ccgggacgga catgacccgg agcatgtggt ggtgacgacc
     2281 ggcctcccca cgtacgcgat cgaagccgag ttggtcgcgg agacgctgcc tgccctggac
     2341 ccgcggccgc ccgcgatgag ctcagccgac acattgggca acatgcgtgt tatggaggcc
     2401 tggagtcgcg cgttgcgctc ggtttgagcc gaaccgccgc gtgcgggctc ggaggacccg
     2461 acagccatga gcgtaacttt caaagccctg tgtcttacat ccctgtgtgc gttgctcacg
     2521 gtcgtgggct gcaacaagag cggtggagcg agtggctctc aaccgagcgg gagcgcggct
     2581 ccggcccagg acaaggtcgt gaagcttgcg ttcgtgacga acaacgcgag cgagttctgg
     2641 aagatcgcgg ctgcgggcgt ccacaagtac gaaaaggagg gcaaggtcca ggtcgacatc
     2701 aagatgccgg cgaacggcac gaccgaggag cagaaccaga tcctcgagaa cctctcgagc
     2761 cagggctacg acgcgatcgc ggtcagcgcc atcgcgccca aagatcaggt tccgattctg
     2821 gatcgggtcg cgctgaagac caagctcatc accttcgact ccgacgctcc ggcttcgaag
     2881 cgcctgcttt acatcggcac caacaactac gaggccggta aggccctcgg caacgagatc
     2941 gtgaagctct tgccgaacgg cgggaaaatg gcggtgttcg tcggcacgct cgcggccgac
     3001 aacgcacagc agcgtttgca aggcattcag gacgtcgtca aagagcacaa gatcgagatc
     3061 gtcgacaagc gcgaggacaa cacggatcga gccaaggcgc gctcgaacgt cgaggacatc
     3121 atcaacgcgc acagcgactt gaagctcgtg gcggggctct ggtcttacaa cggccccgcg
     3181 atcgcagcgg ccatcgaagc gctcggcaag aagggcaagg tcatcgcggc cgtgttcgac
     3241 gaagaggacg gcacgttgaa cgccatcgcc gccgacacca tctcttgcac cgtcgtgcag
     3301 aagccgtttc agttcggcta cctctccagc aagtgggcgc acgatctggc gacccacccg
     3361 gacaccgccc tcgcggcgat ccctgggaac aagagcatcg acaccggagt cgaagtcatc
     3421 aacaaagcga acgtcgcc
//
LOCUS       DASTKS010000331         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1392701, whole genome shotgun sequence.
ACCESSION   DASTKS010000331 DASTKS010000000
VERSION     DASTKS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392701"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFQ35_06920"
     CDS             <1..1020
                     /locus_tag="VFQ35_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9930398.1"
                     /translation="PLKSGKQFKSFGNTTSGWGSGISLDQYLAQKLNPMTTFKSLQVG
                     VHVRDTEVRARISYLAADQPVPPREDPQDVYNALFGAVTGGAGPSQRLLAQRKSVFDL
                     TKGELTRLKKFLGTDDQQKLEAHASSIRDIEQRLTAMPAMMPSTGTTACAKPTLDKTD
                     LTDDDLYLRAGRTQMDMAAAALACDQTRILTFQWSYSESEHLFQFLNINGNHHAISHD
                     FSSSGAGYDNYNKIQTWYVQQFLYFLQKLDSYKEGDRTLLDNCVVLWATEIGESTQHD
                     LTMMPYVLAGTCGGKLKSGRFLDMSSNRKDNNQMLVSLGQAMGDDLTSFGDPSGATGP
                     LNGLT"
     gene            complement(1013..2020)
                     /locus_tag="VFQ35_06925"
     CDS             complement(1013..2020)
                     /locus_tag="VFQ35_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9930399.1"
                     /translation="MSELRVTATTLGDARLRHSAAMIVEVRHLLTLKAIAETGSLTRA
                     AKRRALTLSAISHQIRELEHLLGVTLLERRTKPLALAPAGARLLACADVVLPALAQAE
                     AEAVRLGAARGARLHLALECHSCFEWLLPALDALREAWGELDIDLRLGPRFDPIPALL
                     AREVDAVLGTDRTAHPGVHYEGIFRYEIVLVVPASHPLARKTYVEPAELRDEPFVTYP
                     VEPERLDVVTRFLRPAGVEPKRTRTAELTSVLLQLVKSGRGVAALPSWALFDARDAPE
                     FRTLTLGKAGLHSELFAAVREEERRAPHQVAFLEIARKTAFELLSGITAVERGGGSGA
                     T"
     gene            2079..>2558
                     /locus_tag="VFQ35_06930"
     CDS             2079..>2558
                     /locus_tag="VFQ35_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930400.1"
                     /translation="MITTTTLGYPRIGAERDLKWALENYWSGRSSLSELTHRTASLCS
                     KNLESMRELGLDCVPIGDFSLYDHVLDVAFALNAIPKRVADVKVTHPLQRYFLMARGK
                     VPGLDAELAPLEMTKWFDTNYHYLVPELDGTTRFEYSPGQLAEQFKRAREHGHRARPV
                     "
BASE COUNT          483 a          848 c          805 g          422 t
ORIGIN      
        1 ccgctgaaat ccggtaagca gttcaagagc ttcggcaaca ccacgtccgg ctggggcagc
       61 ggcatctcgc tcgaccagta cctggcgcag aagctgaacc cgatgaccac cttcaagagc
      121 ttgcaggtgg gcgtccacgt ccgcgacacc gaggtgcgcg cccgcatctc gtacctcgcc
      181 gcggatcagc cggtcccgcc gcgcgaggat ccgcaagacg tgtacaacgc cttgttcggg
      241 gcggtgacgg gtggcgccgg tccttcgcag cgcctgctcg cgcagcgcaa gagcgtgttc
      301 gatctcacca aaggcgagct cacccggctc aagaagttcc tcggcaccga cgatcagcaa
      361 aagctcgagg cgcacgcttc gtccatccgc gacatcgagc agcgcctgac cgcgatgccg
      421 gcgatgatgc cgagcaccgg cacgacggcc tgcgcgaagc cgacgctcga caagaccgac
      481 ctcaccgacg acgacctgta cctgcgcgcc ggacgcaccc agatggacat ggccgccgcg
      541 gccctcgctt gcgatcagac gcgcattctg acgtttcagt ggtcgtattc ggagagcgag
      601 cacctctttc agttcttgaa catcaacggc aaccaccacg cgatttccca cgacttttcc
      661 tcgagcggtg ccggctacga caactacaac aagatccaga cttggtacgt gcagcagttc
      721 ctctacttct tgcagaagct cgactcctac aaggaaggcg accgcactct gctcgacaac
      781 tgcgtagtgc tctgggcaac cgagatcggt gagtcgaccc agcacgacct cacgatgatg
      841 ccctacgtgc tcgcggggac gtgcggcggg aagctcaagt cggggcgctt cctcgacatg
      901 agcagcaacc gcaaggacaa taaccagatg ctcgtgtcgc tcggccaagc gatgggcgac
      961 gacctgacga gcttcggcga tccgtccggt gcgaccgggc cgctcaacgg gctcacgtag
     1021 cgccgctgcc gcctccgcgt tccacagcgg taatgccgct caagagctcg aaggcggttt
     1081 tgcgcgcgat ttctaggaac gcgacttgat gtggcgcgcg gcgctcctcc tcgcgcacgg
     1141 cagcgaagag ctcgctgtgt aagccagcct tacccagcgt gagagtgcga aactctggtg
     1201 cgtcgcgcgc gtcgaagagc gcccagctcg gcagtgctgc aacgccgcgg ccactcttga
     1261 cgagctgcaa gagcacgctg gtgagctccg cggtgcgagt gcgcttgggc tcgacgccgg
     1321 cgggcctcaa aaagcgcgtg acgacgtcga ggcgttcggg ctccaccgga tacgtcacga
     1381 acggctcgtc ccgaagctcc gccggctcga cgtaggtctt gcgcgcgagc gggtgcgaag
     1441 cggggacgac gagcacgatt tcatagcgaa agatgccctc gtagtgcacc cccgggtgcg
     1501 cggtgcgatc cgtgccgagc accgcgtcga cctcgcgcgc gagcagagcc ggaatgggat
     1561 cgaagcgcgg gccgaggcgc aagtcgatat cgagctctcc ccaagcctct ctcagcgcgt
     1621 cgagcgctgg caagagccat tcgaaacagc tgtggcactc gagcgccaag tgcaggcgtg
     1681 cgccgcgcgc cgcccccaac cgcaccgctt ctgcttccgc ttgagcgagc gccggcagca
     1741 ccacgtccgc gcacgcgagc agccgcgcac cggccggagc gagcgcgagc ggtttcgtgc
     1801 gtcgctcgag cagagtcacg ccgagaaggt gttcgagctc gcgaatttga tgcgagatcg
     1861 cggagagcgt cagcgcgcgt cgcttggccg cgcgcgtgag gctacccgtt tcggcgatgg
     1921 ccttcagagt gagcaagtgg cgaacctcga cgatcatcgc cgcggagtgt cgcagacgag
     1981 cgtccccgag ggtagtcgcg gtgactctca attcgctcaa gtcgacttga aaaggctgcg
     2041 attgcctcga aattcaggga tagccatgct cgagtgcgat gatcacgacg acgacgttgg
     2101 gctatccgcg catcggcgcg gagcgcgatt tgaagtgggc tctcgagaac tactggtctg
     2161 gacggagctc cttgagcgag ctgacgcacc gcaccgcctc tctctgttcg aagaacctcg
     2221 aatcgatgcg cgaactcggt ctcgactgcg ttccgatcgg cgacttttcg ttgtacgacc
     2281 acgtgctcga cgtcgcgttc gcgctgaatg cgattcccaa gcgcgtcgcc gacgtgaaag
     2341 tcacccaccc gctccagcgt tacttcttga tggcacgcgg gaaagtaccg gggctcgacg
     2401 ccgagctcgc tccgctcgaa atgaccaagt ggttcgacac gaactatcac tacttggttc
     2461 cggagctcga cggcacgacc cgcttcgaat actcgcccgg gcaactcgcc gagcagttca
     2521 agcgtgcacg tgagcacggc catcgcgcac ggccggtc
//
LOCUS       DASTKS010000332         4668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_722100, whole genome shotgun sequence.
ACCESSION   DASTKS010000332 DASTKS010000000
VERSION     DASTKS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722100"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..799)
                     /locus_tag="VFQ35_06935"
     CDS             complement(185..799)
                     /locus_tag="VFQ35_06935"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HET9930401.1"
                     /translation="MLALADRPLDGEVIARSLFDHVITFAWVAAAPSERLPVLVGDDV
                     RHRLAFAEENAKAFGGSMLPDEVKDEFEALRQRLPSAQVMARVADEHWAGVVSNLFRG
                     GSSLSELYSRGFRGYSASVHPTALGIGPFLSSNATGAVVVARPVFHPRRCSFGECTSA
                     MTLLLVISGQALGWPDRGSVTAIWREHPAKRDWLGALAAREPEQ"
     gene            737..1114
                     /locus_tag="VFQ35_06940"
     CDS             737..1114
                     /locus_tag="VFQ35_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930402.1"
                     /translation="MVEQRTRDDFAVKWPVCEREHRLAEVRAAGDTFAVGVADVHAPS
                     GFQFTDVRANSDGRVVVQFDDEGEHVDELDMVRGERLGVPVVAVLAERTTAQVTLFIP
                     AIVTPAEQSRSARAARGVLEEFE"
     gene            complement(1144..1851)
                     /locus_tag="VFQ35_06945"
     CDS             complement(1144..1851)
                     /locus_tag="VFQ35_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930403.1"
                     /translation="MADDETSSPASDDPMWAPIATALRAEMENAPEPGALGAGWQARV
                     TWVRASTMDLMNVQRLYFLGFAVAKEEPARISEPTRSWVAQANTLTSANVQMCILASG
                     LCSILDSVIREMAEAFVQADDVTRVGRKRRLEPDAMKAREQLVRWTSPTAKAEPTTWC
                     ERLGEVFDLPLSDALTTALVSLVTLRHAYIHERAQVLARRPIADEATSWCLAVMLVAA
                     LLGQRMSERAVLRASSP"
     gene            2035..3048
                     /locus_tag="VFQ35_06950"
     CDS             2035..3048
                     /locus_tag="VFQ35_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HET9930404.1"
                     /translation="MVDENKRRAGKARWIGIEKSEQKALVSKAAHARWKKRDVVVAER
                     DGVVNLGGFEINCAVLKDGRRVISERSFCAALGHERRPEEYEKRKQAIEAGEAPLPVF
                     VTPNVAPFLPPGVAEKLSKPIRYQRSEGWGIPAFGVEATLIADICEAYIAAREAGALS
                     PAEEPKARVALTLIRALARVAIVALIDEATGFQVIRDRDELQRLLEQYVSEEFRQWSS
                     AFPDEYYIELFRLRNLTTDDVRHRPQYFGHLTNDIVYDRLVPGMLPRLKEVNPTNEKG
                     RRARKHFQHIKNTVGVEHFERHLAGVVMLMKASSNYAEFKRALDRAAPKRRGLEANKD
                     ADE"
     gene            3041..4351
                     /locus_tag="VFQ35_06955"
     CDS             3041..4351
                     /locus_tag="VFQ35_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9930405.1"
                     /translation="MSEPKNSRGGRPVKGTLGFTRDGRRQPIVTLADGKTRKRLDPFP
                     PGMSMALMLDRTAFYAEKWSKVMPEQPAEPELDPTLPTSAAMAEWFKAWLADRVARGY
                     TATRENSSHFRLHIAPSIGPKHVRDWTRDDLRKLSRELDGKVRANELAWKAAQNVWGT
                     ATKMCDDAAESKSDEIKCRADNPAAGVRGPDRGADTGKQFLYPSEFLSFVRHQDVPLH
                     WRRIVAVAVYTYTRDAELRVLACSDVDVEHLSIRITKALNRRTGATKTTKGKRARALP
                     IEPAVVALLEAMKGERAGDAALVEEMPSERDMARGLRRWLWKAGVRRPELHKATPTTR
                     PIRFHDLRATGITWMAIRGDDPLKIQQRAGHTSFDTTQEYIRLAESMRTGFGDVFPSL
                     PSELYESPGDGGFRSQVSITGSATLRDQKPIWRGGRDSNPRPPA"
     gene            complement(4462..4590)
                     /locus_tag="VFQ35_06960"
     CDS             complement(4462..4590)
                     /locus_tag="VFQ35_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930406.1"
                     /translation="MDLSTACWFGGISVFVVGLLALWERLKRAAPKGRRPRRLLPR"
BASE COUNT          887 a         1532 c         1511 g          738 t
ORIGIN      
        1 cgacttgctc gactgagagc tgctcgaacc gtacgcgctc ggcggccata cgacgtatct
       61 tgcgccctgc tttcggtgaa tgcacgagaa agcagtgaaa gcacgtgctg ccggtgaatg
      121 caggcgcagc caggggcggc accgcccccg ccccccccgc ccccgaatga ccatgcgtga
      181 cccctcattg ctctggctcg cgtgcggcaa gcgcacccaa ccaatcgcgc ttggccgggt
      241 gctcgcgcca gattgccgtg acgctccctc ggtcgggcca gccgagcgcc tgccccgaga
      301 tcacgagcag tagggtcatg gcgctggtgc attcgccgaa cgagcagcgg cgcggatgaa
      361 agaccgggcg agcaacgaca acagcgcccg tggcgttgct gctcaagaag ggccctattc
      421 cgagcgccgt tgggtgaacc gacgcgctgt agcctcgaaa gccgcgcgag tagagctcgc
      481 tcaatgagct acccccacga aagagattgc tgaccacacc agcccaatgt tcgtcagcaa
      541 cgcgggccat cacctgagcc gaaggtaagc gctgacgcaa cgcctcgaat tcgtccttca
      601 cttcgtcggg cagcatgctg ccgccgaatg ccttggcgtt ttcctctgcg aaggcgagtc
      661 gatgccgaac gtcatcgccg actaagaccg gcagacgttc ggaaggagct gccgctaccc
      721 atgcgaacgt gatcacgtgg tcgaacaacg aacgcgcgat gacttcgccg tcaagtggcc
      781 ggtctgcgag cgcgagcacc gactcgcaga ggtacgcgca gcgggcgaca cgttcgccgt
      841 cggtgtcgcc gatgtgcacg ctccatcagg ctttcagttc actgacgtga gagcgaacag
      901 cgatggtcgc gtcgtcgtgc agttcgacga cgaaggcgag cacgtcgacg agctcgacat
      961 ggtccgcggc gaacgcctcg gcgtgcccgt cgtcgccgtg ctcgcggaac gcacgaccgc
     1021 gcaagtgacc ttgttcatcc ctgcgattgt cacgccagca gagcaatcac gctcggcacg
     1081 cgccgcgcgc ggagtgctcg aagaattcga gtgagtcagt agcaggcgcg agacgcgccg
     1141 cactcatggc gaactcgccc gcagtacagc ccgttccgac attcgctggc cgagaagggc
     1201 agcaacgagc atgactgcca agcaccagct agttgcctcg tccgcgatcg gtcgcctggc
     1261 gagcacctgt gcgcgctcgt ggatgtacgc gtgacgcagg gtaacaagcg aaaccagcgc
     1321 cgtggtcaac gcgtcgctca gcggaagatc gaacacttcg ccaaggcgct cgcaccacgt
     1381 tgtcggctcg gctttggctg tcggacttgt ccaccggacc agctgctcgc gtgccttcat
     1441 cgcatccggt tccaggcggc gcttgcgacc aacgcgcgtg acatcgtccg cctgcacgaa
     1501 cgcttcggcc atttctcgta tgacactgtc caggatggag cacagaccac ttgcgaggat
     1561 gcacatctgg acgttcgcgc tggtcagcgt gttcgcctgc gcgacccagc tccgtgttgg
     1621 ctcggagatt cgcgcgggtt cttctttagc aacagcgaag cccaagaagt aaagccgctg
     1681 cacgttcatg aggtccatcg tcgacgcgcg gacccacgtc acccgcgctt gccatcccgc
     1741 gccgagagca ccaggctcag gagcgttctc catctcggcg cggagggccg ttgcgatggg
     1801 cgcccacatc gggtcgtccg aagccgggga gctggtctca tcgtccgcca tgcgggtgcg
     1861 gcctacccgt gctgcgcgcg tgggacgagc cctccatgga agtcccgccg caagcgcatt
     1921 cgtttgctcg caggcccatg tcggccatta ggccgaactg tgtctcactt gcataagtga
     1981 ggcgtcctgc tacattcacc atgcgaacac aattctgctc gcatataggt aaaaatggtc
     2041 gacgaaaaca agcgtagggc cggcaaagct cgatggatcg gaatcgagaa gagcgagcag
     2101 aaggcgctcg tatcgaaggc ggcgcacgcc cgttggaaga aacgcgacgt agtggtcgcc
     2161 gaacgggacg gcgtcgtgaa cctgggcggg ttcgagatca actgcgctgt cttgaaggac
     2221 gggcggcgcg tgatctcgga gcggagcttc tgtgccgcgc tcgggcacga acggcggcct
     2281 gaggagtacg agaagcgcaa gcaagcgatc gaagcgggag aagcgccgtt gcccgttttc
     2341 gtgacgccca acgttgcgcc ttttctcccg ccgggcgttg cagagaagct ctcgaagccg
     2401 attcgctatc agcggtcgga aggctgggga attccggctt ttggtgtcga ggcgacgctt
     2461 atcgccgaca tctgcgaggc gtacattgct gcacgtgaag cgggcgcgtt gagccctgcc
     2521 gaggaaccga aggcgcgtgt agcgttgact ctaattcggg cgctcgcgcg cgttgcgatt
     2581 gtcgcgttga tcgatgaagc gaccggtttt caggtcattc gcgaccgcga cgagcttcaa
     2641 cgtctgctcg agcaatacgt gtccgaggag ttcaggcagt ggtcgtcggc gttccccgac
     2701 gagtactaca tcgagctttt tcgcctgcgg aatctgacca ccgacgacgt gcggcacagg
     2761 ccacaatact tcggtcatct cacgaacgac attgtgtacg accgcctagt tccaggcatg
     2821 ttgcctcggc tgaaggaagt gaatccgacg aatgaaaagg gtcgccgcgc ccgaaagcac
     2881 tttcagcaca tcaagaacac cgtgggcgtc gagcacttcg agcgacacct cgccggtgtc
     2941 gtgatgctga tgaaggcgtc gtcgaactac gccgaattca agcgagctct cgaccgggcc
     3001 gcgcctaagc ggcgaggtct cgaagcaaac aaggacgccg atgagtgagc ccaagaactc
     3061 ccgcggcggc cgccccgtca aaggcacact cggcttcacg cgcgacggcc gacggcaacc
     3121 catcgtcacg ctcgccgatg gcaagacccg caagcgcctc gacccgtttc cgcccggcat
     3181 gtcgatggcg ctcatgctcg atcgcacggc cttttacgcg gagaagtggt cgaaggtgat
     3241 gcccgagcag ccggcagagc ccgagctcga cccgacgctg ccgacgagcg ccgccatggc
     3301 cgaatggttc aaggcgtggc tcgccgaccg cgtggcgcgc ggctacacgg cgacgcgcga
     3361 gaacagctcg cacttccgtc tgcacatcgc accgagcatc ggaccgaagc acgtgcgcga
     3421 ttggacgcgc gacgatctgc gaaagctgtc acgcgagctt gatggcaaag tgcgggcgaa
     3481 cgagctcgcg tggaaggccg cgcaaaacgt gtggggcacg gcgacgaaga tgtgcgacga
     3541 cgcagcggag tcgaagagcg acgagatcaa gtgtcgcgcc gacaaccctg ccgcgggcgt
     3601 gcgcgggccc gaccggggcg cggacaccgg caagcagttc ctctacccga gcgagttcct
     3661 atcgttcgtc cggcaccaag acgtgccgct ccattggcgc cgcatcgtcg ccgtcgcggt
     3721 ctacacgtac acacgggatg ccgagctccg cgtgctggcg tgctccgacg tcgacgtgga
     3781 gcacctgtcg atccgcatca ccaaggcgct caaccgtcgc accggcgcga ccaagacgac
     3841 gaagggcaag cgcgcccgcg ccctcccgat cgagcctgcc gtcgtcgcgc tgctggaagc
     3901 gatgaagggt gagcgggcgg gggacgcggc gctcgtcgag gagatgccga gcgagcggga
     3961 catggcgcgc gggctccggc gctggctctg gaaggccggc gtgcggcgcc ccgagctcca
     4021 caaggcgacc cccacgacgc gcccgatccg ctttcacgac ctccgcgcga cgggcatcac
     4081 gtggatggcc atccgcggcg acgacccgct caagatccag cagcgcgccg ggcacacgag
     4141 cttcgacacg acgcaggagt acatccgcct cgcggagtcg atgcgcaccg ggttcggtga
     4201 cgtgttcccg tcgctgcctt cggagctcta cgagtcaccg ggcgacgggg ggtttcgatc
     4261 acaggtttcg atcacaggat cggcaactct gcgagatcag aaaccaattt ggcggggcgg
     4321 acgggactcg aacccgcggc ctccggcgtg acaggccgca aacggatccg cgcctcgcag
     4381 cctagcaagg ccgcggcgtg agggacgggc ccacgagagg gcccacgagt ctccccgtgg
     4441 gcccgccgtg gtccgcgctt ctcaccgggg cagcagccgg cgcggccggc gccccttggg
     4501 cgcggccctc ttgagccgct cccagagggc gagcagcccc accacgaaca ccgaaatacc
     4561 accgaaccag cacgccgtag acagatccat cgaactctcc agggtcaaac caccccgcga
     4621 accctgggcc taagtgcccg gatttgcaga ggtttcagtc aggggccc
//
LOCUS       DASTKS010000333         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1169092, whole genome shotgun sequence.
ACCESSION   DASTKS010000333 DASTKS010000000
VERSION     DASTKS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169092"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /gene="mnmA"
                     /locus_tag="VFQ35_06965"
     CDS             <1..1008
                     /gene="mnmA"
                     /locus_tag="VFQ35_06965"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9930407.1"
                     /translation="VVGVTLHLWDYPDDGDAKSRCCAPEDVHDARRVADVLGFPHYAF
                     DRRELFASEVVSPFVDAYLEGTTPSPCVRCNRGVKLSELFRLADRLGAARIATGHYAR
                     VVEREGRPELWRARDANKDQSYFLHTLDESRLARLVFPLGDAHKSEVRDEAQAANLPG
                     ATKGESQELCFVSSGRYDSFVADRAGGRVRPGPIVDEGGRVVGTHGGIHGFTVGQRKN
                     LGVALGQRAYVAAIDPANATVRLATGDSLAFYGAALTEVTLFASVTLPLTCEVQVRYR
                     GKPIAAEVRASETGALVRFEAPVGAVSKGQYAVFYDGERVLGGGLITEALGKPPWEVA
                     A"
     gene            1005..1676
                     /locus_tag="VFQ35_06970"
     CDS             1005..1676
                     /locus_tag="VFQ35_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930408.1"
                     /translation="MTRFTLALGWVTAIASAAATGCAVGEGEGSVKSDRLFVEGCWNG
                     TFDLKPDFFAANPDNQGDSLMLRLQRGDNIEEQSDGLLILVNQLSRVRKQLSDSGTGE
                     TTLKLGLPPGVRPPGVPVMANPDPPLVSLSLYLNKSCHAQNTTVYSTSGSITFRSLFS
                     GDPQESSADARLTDASFDATVADPRLLAPSSTHAPPDGVEVESHIAGDFRFYYQRGQP
                     AQPFQ"
     gene            1673..1867
                     /locus_tag="VFQ35_06975"
     CDS             1673..1867
                     /locus_tag="VFQ35_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930409.1"
                     /translation="MKTRVDEVLLRQAQEFSLRFGCEACAHYEDELGGCSNGFPNAPH
                     KAKRLELMSELEFCKQFELG"
     gene            complement(1864..2958)
                     /locus_tag="VFQ35_06980"
     CDS             complement(1864..2958)
                     /locus_tag="VFQ35_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET9930410.1"
                     /translation="MGEPRSRFARLLRLLRGSGSPARVGLSIGLGLFIGCLPLYGFHL
                     PLCLLVCLPFGLDAVLAYLAANISNPLVAPFLVFSEVVVGSWLLHGRAPSFDLQAAKH
                     PDLGLIAREIALGSVVVGGVLGVLGGVIAASIARRRRKGHDDTLAAARQRTIARYAGA
                     PPGDRYYVAAKLHTDPSLTQVAALGPLGDVIDAGCGRGQLSLCLAELGNVGRLSGFDF
                     DARKVEVARGAAGDRAEFRVDDLESVELASADTILFMDVLHYLDVDAQDRLLARAATK
                     LRAGGRIVVREVDAASARKSFLTRGFEFIATRLGYNRTARSLGFRPLADIRAALERLG
                     LSCEASADEPRSLLENRLLVARAPSPTTSG"
     gene            complement(2951..>3540)
                     /locus_tag="VFQ35_06985"
     CDS             complement(2951..>3540)
                     /locus_tag="VFQ35_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930411.1"
                     /translation="IAHSRGDFLAAERHFEKAVSLNPNYTEAQLNLMVTYNDLGKYDR
                     ARDVYSKIRHRGQPGTALADPFAKGKIANMHADVGQAYIDAGMPTEAIHEFERAIALC
                     PEFSDLRTRLAGLYRDSGSSDLAKDQLYLALEHNPSYAPARVMLGVLLLSSGDYGAAL
                     SEFESALATDPDNRSAQMYSRLARVHRDKSQRPSG"
BASE COUNT          605 a         1160 c         1223 g          552 t
ORIGIN      
        1 gtggtgggcg tcacgctgca cttgtgggac taccccgacg acggcgacgc aaagagccgc
       61 tgctgtgcgc cggaggatgt tcacgacgct cggcgcgtcg ccgacgtgct cggctttccg
      121 cactatgcct tcgaccgacg cgagctgttc gcgagcgagg tcgtgagccc cttcgtcgac
      181 gcttacctcg aagggacgac tccgagcccg tgcgtgcgct gtaaccgcgg cgtgaagctc
      241 agcgagctgt tccgcctcgc cgatcgcctc ggcgccgcgc gcatcgcgac gggacattac
      301 gcgcgcgtgg tcgaacgcga gggccgtccc gagctctggc gcgcgcgcga cgccaacaaa
      361 gaccaaagct actttctgca cacgctcgac gagtcacggc tcgcccggct cgtgttcccg
      421 ctcggcgacg cgcacaagag cgaagtgcgc gacgaggccc aagccgcgaa cctccctggc
      481 gcaacgaagg gcgaaagcca ggagctttgc ttcgtttcga gtggacgcta cgactctttc
      541 gtcgccgacc gcgcgggcgg gcgcgtgcgc ccggggccaa tcgtggacga aggcggtcgc
      601 gtagtgggca cgcacggcgg catccacggc ttcacggtgg gacagcgcaa gaacctcgga
      661 gtcgcgctgg ggcagcgcgc ctatgtcgcc gcgatcgatc cggcaaacgc gaccgtccgg
      721 ctcgcgaccg gcgactcgct cgcgttctac ggcgcggcgc tcacggaggt cacgctgttc
      781 gcgtcggtca ctcttccgct cacgtgtgaa gtccaggttc gctaccgggg gaagcccatc
      841 gccgcggagg tgcgcgcgag tgaaaccggc gcgctcgtgc gtttcgaggc gcccgtcggc
      901 gccgtctcca aggggcagta cgccgtcttc tacgacggag agcgcgtact cggcggcggc
      961 ctcatcaccg aggcgctcgg caaacctccg tgggaggtcg cggcgtgacc cgcttcacgc
     1021 tcgcgctcgg ttgggttacc gccatcgcga gcgccgcggc gacgggctgc gccgtcggcg
     1081 aaggcgaggg ctcggtgaag agcgatcgcc tgttcgtgga aggctgctgg aacggtacct
     1141 tcgatttgaa gccggacttc ttcgccgcga accccgacaa ccagggcgat tcgttgatgc
     1201 tgcgcctgca gcgcggcgac aacatcgaag aacaatcgga cggcttgttg atcttggtaa
     1261 atcagctctc gcgcgtcaga aagcagctct ccgattcggg cacgggcgaa acgacgctga
     1321 agctcgggct gcctcccggc gtgcggcctc ccggcgttcc ggtgatggcg aatccggacc
     1381 cgccgctcgt cagcttatcg ctctacttga acaagtcgtg ccacgcgcag aacacgaccg
     1441 tgtattcgac ctctggctcg atcacgtttc gctctctgtt cagcggcgat ccacaggagt
     1501 cgagcgccga cgctcgcctg acggatgcga gcttcgacgc gaccgtcgcc gacccgcgcc
     1561 tgctcgcgcc gagctcgacc cacgcgccgc cggacggcgt ggaagtggag agccacatcg
     1621 ccggcgactt tcggttctat taccagcgcg gtcaaccggc gcagccgttt cagtgaagac
     1681 gcgtgtcgac gaggtgctgc tgcgccaggc gcaggagttt tcgctgcgct tcgggtgcga
     1741 agcgtgcgcg cactacgagg acgagctcgg tggctgcagc aacggcttcc ccaacgcgcc
     1801 tcacaaagcg aagcggctcg agctgatgag cgagctcgag ttctgcaagc aattcgagct
     1861 cggttaaccg gaagtcgtag gcgacggcgc gcgcgccacg agcaagcgat tttcgaggag
     1921 cgaacgcggc tcatcggcgg acgcttcgca gctaaggccg agccgctcga gcgccgcacg
     1981 gatatcggcg agggggcgaa acccgagcga gcgggcggtg cggttgtagc cgaggcgagt
     2041 cgcgatgaac tcgaagccgc gggtgaggaa gctctttcga gccgacgcgg cgtcgacttc
     2101 acgaacgacg atgcgccccc cggcgcgcag cttggtcgcg gcgcgtgcga gcaagcgatc
     2161 ctgcgcgtcg acgtcgaggt agtgcagtac gtccatgaag aggatcgtgt ccgcgctcgc
     2221 gagctcgacc gattcgaggt cgtcgacgcg aaactcggcg cgatcgccgg cagcgccgcg
     2281 agccacctcg actttgcgcg cgtcgaaatc gaagccgctc aagcgcccga cgttcccgag
     2341 ctcggcgaga cagaggctga gctgcccgcg gccgcagccg gcgtcgatca cgtccccgag
     2401 cggtccgagc gcggcgacct gcgtgagcga tgggtcggtg tggagcttgg ccgctacgta
     2461 atagcgatcc cctgggggcg cccctgcgta ccgggcgatg gtgcgttggc gagccgcggc
     2521 gagcgtgtcg tcgtgaccct tacgacgacg ccgggcaatc gatgcggcga tgacgccccc
     2581 gagcacgccc agcactccgc cgaccacgac gctgccgagc gcgatctccc gcgcgatgag
     2641 gccgaggtcg gggtgcttcg cggcttgcag atcgaagcta ggagcgcgcc catgcagcag
     2701 ccaggagccg accacgactt cggagaacac gagaaacggc gctaccagcg ggttcgagat
     2761 gttcgcggcg aggtacgcga gcaccgcgtc gagaccgaac ggcaagcaca cgagcaagca
     2821 cagcggcagg tgaaagccgt agagcggcag gcacccgatg aagagcccga gcccgatgct
     2881 gagcccgacc cgcgctggcg agccgctccc acggagcagc cgcagcagcc gagcgaagcg
     2941 cgagcgaggc tcacccactc gggcgctggg acttgtcacg atgcacacgc gcgaggcgcg
     3001 agtacatctg ggcgctgcga ttgtcgggat cggttgcgag cgcactttcg aattcactga
     3061 gcgcggcgcc gtagtcgccg gagctgagca agagcacgcc gagcatcacg cgcgctggcg
     3121 catacgacgg gttgtgctcg agcgccaagt agagttggtc cttcgcgagg tcggagctgc
     3181 cgctgtctcg atacagtccc gcgagccgcg tgcgcaaatc cgagaactcg gggcagagcg
     3241 cgatggcgcg ctcgaactcg tgaatcgctt cggtaggcat gcccgcatcg atgtacgcct
     3301 gaccgacgtc cgcgtgcatg ttcgcgatct ttcccttcgc gaacggatcg gcgagcgcgg
     3361 tgccgggctg accgcggtgc ctgatcttcg aatacacgtc gcgcgcgcga tcgtatttac
     3421 cgaggtcgtt gtaagtgacc atcaggttga gctgcgcctc ggtgtagttg ggattcaacg
     3481 aaaccgcttt ctcgaagtga cgctcagcgg ccaagaagtc tccgcggctg tgagcgatca
//
LOCUS       DASTKS010000334         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_945859, whole genome shotgun sequence.
ACCESSION   DASTKS010000334 DASTKS010000000
VERSION     DASTKS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945859"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFQ35_06990"
     CDS             complement(<1..373)
                     /locus_tag="VFQ35_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930412.1"
                     /translation="MMSPRLASCFLVCAIACTRRASSPAEAGGDAAPSSPPPAPARPE
                     ELKQMCDAGGLPQIDETAVGAEPLLTAAERGTVALRWDGAHAAFTEGCSLPGAYTELP
                     GSGGGRFFGTNRVTLRVDEVPP"
     gene            complement(370..>2951)
                     /locus_tag="VFQ35_06995"
     CDS             complement(370..>2951)
                     /locus_tag="VFQ35_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930413.1"
                     /translation="QQGYFELPDAGDVTFFDGMFLNVDIKQVQLGSREAPLELRLKLR
                     WQFFRFDYFDRFSSTFASVPQARKDAKGNAVRPFILEGTSKLVDADGFMSDTLARSAW
                     ELTVGKTPVHCLGWPVREVLADDQRRELPDANCTVGFLGRDLFVRTDGDGTSPDAPRL
                     TILVNPGDDVLSKVGIERLRLYDLPIEWRSRFYPARIAGEKVDQSRPFEQVAATASTK
                     DKPYVVSLDVIVMAKAGLPVAADTGIVLDDSTLSNRFAILDNNMNVFKPEPASGSPFF
                     TRAADLKPPPASGVLVDLPPFTRVIVRGRHLYDVFDERTRANDRFKGAPVGARLALPL
                     ADGDDRSSTRVSSAPFRAPRPNSHFTPTRLGEGRVALFRLCGHTGGVELFVFFQYVRA
                     SFIFPKPLNPTHRTRFGLLANAVELDSPPPNAANLVRDCLLGIGKRWSGEDSVNSTTT
                     TFEIGSPVVARGRYVARLHPGDRTTEPTEEGEFLVTILKKQRAFMAGSGKLGFWELGN
                     MSPSGDSFTAAHEFGHTFAQPDEYFNTDDEPSYRLNNIGEVARSPGTPYGPDATAMMT
                     GGEAQPRARYFWDLLLFARDNGHFAGQANFVVKRGAITLSPEVTAPGQTRTRLPVAQK
                     LNVSIPPLGLCDLFIYLMGHDEFTARDIDEQATAKPYDGIVMVRVKLNFSFATTSDFS
                     DISTLMGRASAAISGAFNTTPLALQGVVGGKSVRLRVKFAPRFVGSTFPTGSGADKYL
                     ASIGLSKPATATTPAVVATPATYAALVSSNISTHGVHANVTVGNFASPSLTAGTPRAA
                     QVRLDGSILGIFTNPRFDGDVVNVFAQLLGMAGAPKKALDFAPLLAALTPEVTAAVAT
                     FNP"
BASE COUNT          540 a          938 c          985 g          488 t
ORIGIN      
        1 acggcggcac ctcatcgacg cgcagagtga ctcggttcgt gccgaaaaag cgacctcctc
       61 cggacccggg caactccgtg tacgctcctg gcaacgagca cccttcggtg aacgccgcgt
      121 gcgcaccatc ccaccgcaat gcaacggtcc cgcgctcggc cgccgtgagg agcggctccg
      181 cgcccacggc ggtctcgtcg atttggggta gcccgccggc atcgcacatc tgcttcagct
      241 cttccggcct cgcgggtgcg ggtggcggcg agctcggcgc ggcgtccccg cccgcttccg
      301 ccggagacga ggctcggcgg gtgcacgcga ttgcgcacac gaggaagcac gacgcgagtc
      361 tcgggctcat catgggttga acgtcgccac cgcagcggtg acttccggcg tcaaagcggc
      421 cagcaacggc gcaaagtcta gcgccttctt gggcgcgcca gccatgccca agagctgagc
      481 gaacacgttc accacgtcgc cgtcgaagcg agggttggtg aagatcccca gaatcgaacc
      541 gtcgagacga acctgagccg cgcgcggcgt gcccgcggtc aggctcggtg aagcaaaatt
      601 cccgacggtc acgttggcgt gcacgccgtg cgtgctgatg ttcgacgaaa ccagagccgc
      661 gtaagtagct ggcgtcgcca ccaccgcagg agtcgtcgct gtagcgggct tggaaagacc
      721 aatggaggcg aggtacttgt ctgcgccgct accagtcgga aaggtggagc cgacgaagcg
      781 cggcgcgaac ttcacgcgca ggcgtaccga tttgccgccc acgacgccct gtagcgcgag
      841 cggggtcgtg ttgaaggcac ccgaaatcgc cgcggaagct cgccccatga gagtggagat
      901 gtcgctgaaa tcgctggtcg tcgcgaagct gaagttcagc ttcacgcgga ccatcacgat
      961 accatcgtac ggcttcgccg tcgcctgctc gtcgatgtcg cgcgccgtaa actcgtcgtg
     1021 gcccatcagg tagatgaaca agtcgcacag cccgagcggc gggatgctca cgttgagctt
     1081 ctgcgcgaca ggcaagcggg tgcgcgtctg gcctggcgcc gtcacctccg gcgaaagggt
     1141 gatcgcaccg cgcttgacga cgaagttcgc ctgcccggcg aagtgcccat tatcgcgagc
     1201 gaacaacaac aagtcccaga agtaacgggc ccgcggctgc gcttcgccgc ccgtcatcat
     1261 ggcggtcgcg tccgggccgt acggcgtgcc ggggctccgc gcgacctcgc cgatgttgtt
     1321 gaggcggtac gaaggctcgt cgtcggtatt gaagtactcg tccggctgcg caaacgtgtg
     1381 cccgaattcg tgcgcggccg tgaagctatc gccactaggg ctcatgttcc cgagctccca
     1441 aaatccgagc ttcccggacc ccgccatgaa ggcgcgctgc ttcttcaaga tcgtgacgag
     1501 aaactcacct tcttcggttg gctcggtggt cctgtcgccg ggatgcaaac gcgcgacgta
     1561 acgcccgcga gcgacgaccg gactgccgat ctcgaaggtg gtcgtggtcg agttgaccga
     1621 gtcttccccg ctccagcgct tgccgatgcc cagcaaacag tcgcgtacga ggttcgcggc
     1681 gttgggcgga gggctgtcga gttcgaccgc gttcgccagc aagccgaagc gagtgcggtg
     1741 cgtagggttc aagggcttcg ggaaaatgaa gctcgcgcgc acgtattgaa agaagacgaa
     1801 gagctccacg ccgccggtgt gcccgcacaa gcgaaacaag gccacgcgcc cctctcccag
     1861 ccgcgtcggc gtaaagtgcg agttggggcg tggcgctctg aaaggcgcgg atgatacgcg
     1921 ggtcgaagat cggtcgtctc cgtcggcgag gggtagcgcc aatcgggcac ccacaggtgc
     1981 tcccttgaat cggtcgttgg ctcgcgtgcg ctcgtcgaac acgtcgtaaa ggtgccggcc
     2041 gcgcacgatg acgcgcgtga acggcggcaa atcgacgagg acccccgagg ctggcggcgg
     2101 cttcagatcg gcggcgcggg tgaagaacgg gctgcccgaa gccggctcgg gcttgaacac
     2161 gttcatgttg ttgtcgagaa tcgcaaagcg attcgagagc gtgctgtcgt cgagaacgat
     2221 gccggtgtcg gcagcgacgg gcaagccggc cttggccatc acgatgacgt ccaacgagac
     2281 gacgtagggc ttgtccttcg tgctggcggt ggctgccact tgctcgaaag gtcgcgattg
     2341 gtcgaccttc tcgcctgcga ttcgcgccgg gtagaaccgg gaacgccatt cgatgggcag
     2401 atcgtagagg cgcagccgct cgattccgac tttgctgagg acgtcgtctc ccgggttcac
     2461 caagatggtg agccgcggag catcgggcga cgtgccgtcg ccgtcggtgc gtacgaacaa
     2521 atcgcgcccc agaaagccga cggtgcaatt ggcgtccggc aattcacggc gctggtcgtc
     2581 ggcgaggacc tcgcgaaccg gccagcccaa gcaatgcacc ggcgttttgc cgaccgtgag
     2641 ctcccacgcc gagcgcgcca gcgtgtcgga catgaagcca tccgcgtcga cgagcttcga
     2701 cgtgccttcc aaaatgaagg ggcgcaccgc gttgcctttc gcgtctttgc gagcctgcgg
     2761 cacgctcgcg aacgtcgagc tgaagcggtc gaaatagtcg aagcggaaga actgccaacg
     2821 gagcttgagc cgaagctcga gcggagcctc ccgactaccg agctgcactt gcttgatgtc
     2881 gacgttcagg aacatgccgt cgaagaacgt cacgtcgccc gcgtcgggca gctcgaaata
     2941 accctgctgg g
//
LOCUS       DASTKS010000335         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1169319, whole genome shotgun sequence.
ACCESSION   DASTKS010000335 DASTKS010000000
VERSION     DASTKS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169319"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="VFQ35_07000"
     CDS             <1..2049
                     /locus_tag="VFQ35_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930414.1"
                     /translation="EGMLTTWQQTSVMDYPGDVTQDMLDIGAYDRAAIRFLYADVADI
                     WNDPRVSCDVRRDGNGAASVNGPTMGTVSKCTDDGGLVSDQLDSFGGITGSWYGASAG
                     IDKVIHYSKLDQRLDLIRDCRAADPTPPADWNEEENGVYDPTIDGQVVNGTICSGIPT
                     DYVRYADLTAVPSRDGPTRKFDELGRVRRPYMFASDEYADIGNIAVYRHDNGADAYEI
                     ASYFINEYEDIHAFDNHRRGRTTFTLSGAFMRSFTRYHEKLKEMGKAYALLQEIFSGA
                     TSLGYPDIAAVDGLSKPHALAASLTFDHIARMLTRPTAGDHFLTPEADPLGHQVLIST
                     DQSIIVNGTNNAPNLTISEGTQNPGVAPFLGGRPLHNALDGSKGYYATRYDTWVGSYY
                     EKTLAMDLLADSIDRFISQSRDDFSDGRYRNVSFATLFPEGVRRLVAASLTQDADLLG
                     WRVAAVGTKPRVQSGTRLPLSPLGFRVWWPADGPKICWPAPGQLGCGDTASGAANGTA
                     PATSIAIDPEVGFEVQKFIAFFSLVYLPDSWKRDWVDLMRIYQIGTNSNPGLDSAVGF
                     RDPLSGEVYVARSYGKETIEGRIVDRGIGAHVLEWANTLAAAAYKVESTAPSGEHTYA
                     KYTANDTCPPGLPQCVGQPIRQDARYAAKLAGYKSVIEFARSLADSMGFGDPYWRGVN
                     "
     gene            2031..2861
                     /locus_tag="VFQ35_07005"
     CDS             2031..2861
                     /locus_tag="VFQ35_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930415.1"
                     /translation="MEGSQLKLGFPRRFVSLLFIASVPLACERAAPSSKRAQPSASAH
                     VVDPKAYPRERLVQALAAVQARWQSLGALPDCVALLAKADERERCAAAQKQLAARQAA
                     ALAPNTTDATRLELATQVALSSQRASESLRASGVNRLLETRPKPSASGSASPPAASAA
                     RAKTVAVASAARAKTIAAASAVATKAAPTREQSDPYLDAIQAFARVTTVALAEVRAYL
                     EFGDNALRSVALTRVETLVSEQPHWAALASLVNEAHLVESEPALKQRLGALRARLGIQ
                     "
     gene            2980..>3468
                     /locus_tag="VFQ35_07010"
     CDS             2980..>3468
                     /locus_tag="VFQ35_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930416.1"
                     /translation="MRWLQALGGLLLLGACSSGEYTSGGATGGMAGASSALGGNSGEP
                     SSGGKHTDGSGGLASGGGITSAGGMGGATSGGSGDGGGATSAGGSGGGGGATSAGGSG
                     GAGGGAQVACGTAGLTCDAGSLCVESTIYPNNVDPAHTYPPSTSWQCKTNPCGDAPLS
                     CDC"
BASE COUNT          622 a         1139 c         1130 g          577 t
ORIGIN      
        1 gagggcatgc tcaccacttg gcagcagacc agcgtgatgg actaccccgg cgacgtcacg
       61 caggacatgc tggacatcgg cgcctacgac cgcgccgcga tccgcttctt gtatgccgac
      121 gtcgcggaca tctggaacga cccgcgcgtt tcgtgcgacg tgcgccgcga cggcaacggc
      181 gcagccagcg tgaacggccc gaccatgggg acggtcagca agtgcaccga cgacggcggc
      241 ttggtttcgg atcaactcga ctcgttcggc ggcatcaccg ggagctggta cggcgcttcc
      301 gccggaatcg acaaggtcat ccattactcg aagctcgatc agcgactcga cttgattcga
      361 gactgcaggg ccgctgatcc gacgccgccc gccgattgga acgaggagga gaacggtgtc
      421 tacgacccga ccatcgacgg ccaggtcgtc aatgggacga tttgttccgg cattccaacc
      481 gactacgtcc gctacgcgga tctcaccgcg gtgccgtcac gcgatggccc cactcgcaaa
      541 ttcgacgagt tgggccgcgt tcggcgacct tacatgttcg cgtcggacga gtacgccgat
      601 atcggcaaca tcgccgtcta tcgtcacgac aacggcgccg atgcctacga gatcgcgtcg
      661 tacttcatca acgagtacga agacattcat gcattcgaca accaccgccg cggccgcact
      721 acgttcaccc tgagcggcgc gttcatgcgc tcgttcacgc ggtaccacga gaagctcaag
      781 gagatgggca aagcctatgc gttgctccag gagatcttct ctggcgcgac ctcgctaggg
      841 tacccggaca tcgcagcggt agacggtctc tcgaagcctc acgcgctcgc ggcgagcctc
      901 acgttcgacc acatcgcgcg catgctgacc cgaccgacgg cgggcgatca cttcctcact
      961 cccgaagcgg acccgctcgg tcatcaggtg ctcatctcga ccgatcagtc gatcatcgtg
     1021 aacggcacga acaatgcgcc aaacctgacg atttcagagg ggacgcagaa ccccggcgtt
     1081 gcgccgttcc tcggcggcag gccgctgcac aacgcgctcg acgggtccaa gggctactac
     1141 gccacgcgct acgatacctg ggtcgggtcc tactacgaga agaccttggc gatggaccta
     1201 ctcgcggact cgatcgaccg attcatcagt caaagccgcg acgacttctc cgatggtcgt
     1261 tatcgcaacg tgagcttcgc gacgctgttc ccggagggcg tccgccgact ggtcgcggcc
     1321 tcgctcactc aagacgcgga tctgctgggc tggcgcgtcg ccgcggtcgg aaccaagccg
     1381 cgcgtgcaat ccggcacacg cctgccgctg agtccgctcg gcttccgcgt gtggtggcca
     1441 gcggatggac caaagatctg ctggcccgca ccgggacagc taggttgcgg agacaccgcg
     1501 agtggcgcgg ccaatggaac cgcgcccgcc acctcgatcg ccatcgatcc agaggtcggt
     1561 ttcgaggttc agaagttcat cgcgttcttc tcgctcgtgt acctgcctga cagctggaag
     1621 cgcgactggg tcgatttgat gcgcatctat cagatcggta ccaactcgaa ccccgggctc
     1681 gattcggcgg tgggcttccg cgacccgctc tcgggcgagg tttacgtcgc gcgcagctat
     1741 gggaaggaga ccatcgaagg gcggatcgtc gatcgcggaa tcggcgcgca cgtgctcgaa
     1801 tgggccaata cgttggccgc agccgcctac aaggtcgagt cgactgcgcc cagcggggaa
     1861 cacacttacg cgaagtacac cgcaaacgac acctgcccgc caggcttgcc gcaatgcgtg
     1921 gggcaaccga tacgccaaga cgctcgctac gccgcgaagc ttgccggtta caagagcgtg
     1981 atcgagtttg cgcgcagcct ggccgactcg atggggttcg gggatcccta ttggagggga
     2041 gtcaactgaa gctcgggttt ccgcgtcggt tcgtaagcct gctcttcatc gcctcggtgc
     2101 cgctcgcgtg tgagcgggcg gcgccgagct cgaagcgtgc gcagccgagc gcgagcgcac
     2161 acgtcgtgga tcccaaggca tacccgcgcg aacggctggt gcaggctctc gctgcggtgc
     2221 aagcgcgctg gcagagcctt ggcgcgttgc ccgattgcgt tgctctgctc gcaaaggccg
     2281 acgaacgaga gcgatgcgcg gccgcccaga agcagctcgc ggctcgacag gcggctgcgc
     2341 tggccccgaa cacgaccgac gcgactcgcc tcgagctcgc aacgcaagtc gcgctgagct
     2401 cgcaacgcgc cagcgagtcc ctgcgagcga gcggcgtcaa tcgcttgctc gaaactcgcc
     2461 caaagccgag cgcttcgggt tccgcatcac cgcccgccgc gtccgccgct cgagcgaaga
     2521 cggtggcggt cgcgtccgcc gctcgagcga agacgatcgc ggcagcgtcc gccgtagcca
     2581 ccaaagcggc tccaacccgc gagcagagcg atccttacct cgacgcgatt caggcgtttg
     2641 cgcgcgtcac caccgtcgcg ctcgccgagg tccgcgctta tctcgaattc ggtgataacg
     2701 ctctacgcag cgtggcgctc actcgggtcg aaacgctcgt cagcgagcag ccccattggg
     2761 ccgcgctcgc gtcgctcgtc aacgaggcgc acctcgtcga gagcgagcca gccctgaagc
     2821 agcggctcgg cgcgcttcgg gctcgcctcg gcattcaatg atttacccaa cctagagcca
     2881 tagcctggcc caagttgtgc cagctgcgcc cggcagcggg cgcaagtgcc gcagatttgc
     2941 gctgaacaca gcggcacgat gcctgcaaag cagtcctaaa tgcgttggct acaggcgttg
     3001 ggtggtctgt tgctcctcgg agcgtgctcg agtggcgaat acacctcggg cggagcaacg
     3061 ggcgggatgg cgggcgcctc gagcgcgctt ggtgggaaca gtggagagcc gtcaagcggt
     3121 ggcaaacaca ctgacggcag cggtggtctg gccagcggag gcgggatcac cagcgccggc
     3181 ggcatgggcg gtgcgacgag cggaggttct ggtgacgggg gcggtgcgac gagcgccgga
     3241 ggttctggtg gcgggggcgg tgcgacgagc gccggaggtt ctggtggcgc gggtgggggc
     3301 gcgcaagttg cgtgcggtac ggccgggctc acctgtgatg caggctcgct ttgcgtcgag
     3361 tcgacgatct atcccaacaa cgtagatcct gcgcacacct atccgccttc cacgtcgtgg
     3421 cagtgcaaaa cgaatccttg cggtgacgca ccgctctcgt gcgattgc
//
LOCUS       DASTKS010000336         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1169380, whole genome shotgun sequence.
ACCESSION   DASTKS010000336 DASTKS010000000
VERSION     DASTKS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169380"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2686
                     /locus_tag="VFQ35_07015"
     CDS             <1..2686
                     /locus_tag="VFQ35_07015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9930417.1"
                     /translation="DSFVLQVPLALDEPGQRGRARRNVPRCNFENARTFVAQGLKRFV
                     LEPLEFITLSGNIPGALFAALPRHLPIAFGAGSVRLRTRDGSLVPLGPRGSADVLAEM
                     IAALVYHYDPDRDGGTALTDVFINDGDFAVMRLGNGALDVRLTAARHRESGIGRNLFI
                     SYLLQMMAYEDFTIDGSLTGVPLLVSNPAVTFEGLVRGLRHRFSDLDRSPEAGVVEAR
                     RWIAEFAGSREGRAYRRFAEAFLEGRLELTFGEDLREHWWRRFPLERKQNLLELRSRV
                     DPLSGAADSARLLKTFIERLGREVGRLPEDPTAIYFNDLDGEGLRGLLDEARIDEAGR
                     ERIVPQVFEHWPWRALDSLLGRVPEARGLRRFKSRLTFGDAISPNDEGTLRSLGAPPK
                     LGPSRPVANRELFDAHFAPAGLAAAALSTFPTFETYMDAALHDADFGYYARRVVIGQG
                     GHFDTHPEELSPHYGEWIASLAFKAWRDLVQHGELTAEEPFSLVEFGAGNGRLARDVL
                     DFVSRASTSPAVLQVESWKAFASCLRYRIYEMSASLRERQASLLGERATICEGDARSP
                     NAALSRDFPLGLKGLVVTNEVPDAFGVHKVVLTAEGDGYAALVVPRVEESLVAALGRE
                     ISERASSSDATIRERFGFSSSDSELYLDDEGLHALLRTVANLESHERDERLGSLWFEE
                     LYVPVSALPALANHFATNADDYANALAAEPSGVVVYVNLHAERFVQELGATLGAGFVL
                     TIDYGETTWGLVQNARRGDFPFRVYGDQLPFVPRPNDPYTTPGSQDLTADVNFSALAN
                     AGRRAGLEVIHFGPERDIVGDDVLPDVLRAAARDPFAKFLGHPGFKTLLLGKRASAAF
                     DSRFATALALSAREQDVPKARRDQRASIHRRLSGLGAR"
     gene            complement(2709..>3211)
                     /locus_tag="VFQ35_07020"
     CDS             complement(2709..>3211)
                     /locus_tag="VFQ35_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930418.1"
                     /translation="EPGRAAIECESMTCEIISGNGAVFSVWGRPSRGDIDRVEEAMRS
                     TKSSSGDRITYVTRVPVDAPAPDAEVRRHLDSRMPVVVDHCTTYHVILEGGGFVAALK
                     RSVLVSIFQVRWRNGTFFVHRDCDEVLDEVSPERRAGVAALLGRARQLGLLTQTPGVT
                     RVWPTP"
BASE COUNT          541 a         1109 c          989 g          572 t
ORIGIN      
        1 cgacagcttc gtactccagg tgccgctggc gctcgacgaa ccgggacaac gaggccgcgc
       61 gcgacgcaac gtcccgaggt gcaacttcga aaacgcacgg acgttcgttg cgcagggatt
      121 gaagcgcttc gtgctcgagc cactcgagtt catcacgctc tccgggaaca tacccggcgc
      181 gctctttgcc gcgctcccga ggcacctacc catcgcgttc ggagccggca gcgttcgcct
      241 gcgaacgcgc gacggatcgc tcgtcccgct cggtccgcgc ggaagtgccg acgtgctcgc
      301 ggaaatgatt gctgcgctcg tttaccacta cgaccccgac cgcgacggag gcaccgcgct
      361 cacggacgtc ttcatcaacg acggcgactt cgcagtcatg cggctcggca acggcgcgct
      421 cgacgtaagg ctcacggcag cgcgacaccg tgagtctggt atcgggcgca acctcttcat
      481 ctcgtatttg ctccagatga tggcatacga ggacttcacc atcgacggga gcctcaccgg
      541 cgttcccctg ctggtctcga acccggccgt gacgttcgaa gggctcgttc gaggtctccg
      601 tcatcgcttc agcgatttgg accggtctcc agaggcaggc gtcgtcgaag cgcgccgctg
      661 gattgcggag tttgccggtt cgcgcgaagg gcgagcttat cggcgctttg cggaagcttt
      721 cctggaaggc cgattggaac tcacgttcgg cgaagacttg cgcgagcact ggtggcggcg
      781 cttccccctc gagcgcaagc aaaacctgct cgagctgcgc agccgggtcg acccgctctc
      841 gggcgccgca gacagcgcgc ggcttctcaa aacgttcatc gaaaggctcg ggcgagaggt
      901 gggccgcttg cccgaagatc cgaccgccat ttacttcaac gacctcgatg gcgaagggct
      961 tcgagggctg ctcgacgagg cgcggatcga tgaggccggg cgcgagagaa tcgtacctca
     1021 ggtgttcgag cattggcctt ggcgcgctct ggactccttg ctcggacgcg tcccggaggc
     1081 gcgcgggcta cgccgattca agagccgcct tacctttggc gatgccatct cgccgaacga
     1141 cgaggggaca ttgcggagcc tcggcgcgcc gccgaaactc ggcccgagtc gacccgtcgc
     1201 caatcgcgag ctcttcgacg cccatttcgc tcccgcaggc ctggccgccg ccgcgctctc
     1261 gacctttccc accttcgaaa cgtacatgga cgcggccctc cacgacgccg atttcggcta
     1321 ctacgcgcgg cgcgtcgtga tcgggcaagg cggccatttc gatacccacc cagaggagct
     1381 ctctccccac tacggagaat ggatcgcaag cttggcgttc aaggcgtggc gcgacctcgt
     1441 gcagcacggc gaactcaccg cggaagaacc cttttcgctg gtcgagtttg gagccggcaa
     1501 tgggcgcctc gcccgcgacg tgctcgactt cgtgagccgc gcgtccacga gcccggccgt
     1561 actccaagtc gagagctgga aggctttcgc ctcgtgcctg cgttatcgca tctatgaaat
     1621 gtcggcttcg ctgcgcgagc gacaggcgtc gctgctcggg gagcgcgcga cgatttgcga
     1681 gggcgacgct cgctccccga acgcggcgtt gtcacgcgac ttcccgctcg gcctgaaggg
     1741 gctcgtcgtg accaacgagg tacccgacgc gtttggcgtc cacaaagtcg tgctcacggc
     1801 cgaaggcgac ggctatgccg cgctcgtcgt tccgcgcgtc gaggagtcgc tcgtggccgc
     1861 gctcggccgc gaaatttcgg agcgagcctc tagttcggac gcgaccattc gtgaacgttt
     1921 cgggttctcc tcgagcgact cggagctcta cctcgatgac gaaggtctcc acgcgctcct
     1981 ccgcactgtc gcgaacctcg agtcccacga gcgcgacgaa cgcctcggct cgctttggtt
     2041 cgaagagctc tacgtccccg tctcggcgct ccccgcactc gcgaaccact ttgccacgaa
     2101 cgccgacgac tacgccaacg cgctcgcggc cgaaccgtct ggcgtcgtcg tctacgtcaa
     2161 tcttcacgcc gagcgtttcg tgcaggagct aggagccacg ttgggcgcag ggttcgtgct
     2221 cacgatcgac tacggtgaaa ccacgtgggg actcgttcag aacgcgcggc gaggagattt
     2281 cccattccgc gtctacgggg accagttgcc gttcgtcccg cgtccgaacg acccctatac
     2341 gacgcccggc agccaagatc tcaccgccga cgtcaacttc agcgctcttg ccaacgcggg
     2401 ccgccgcgcc ggactcgagg tcatccattt cggtcccgag cgcgacatcg tcggagatga
     2461 cgttctgccc gacgtcctgc gcgcggccgc gcgcgacccc ttcgcgaagt ttctggggca
     2521 ccccggcttc aagacgttgc tgctcggaaa gagagcgagc gctgccttcg actcgcggtt
     2581 cgctacagcg ctcgcgctgt ccgctcgcga gcaagacgtt cccaaggctc ggcgcgacca
     2641 gcgcgcctcc atccatcgac ggctctccgg gctcggagca cgctgagcgc gctcgaggcc
     2701 agagcccttt acggcgtagg ccagaccctc gtcacgccgg gcgtttgcgt caacagaccg
     2761 agctgacgag ctcttcccaa tagagctgcc acgcctgcac gtcgctcggg cgaaacctcg
     2821 tccagcactt cgtcgcaatc gcgatgcacg aagaaggtgc cgttgcgcca gcgaacctga
     2881 aaaatgctga ccagcacgct gcgcttcagc gccgcgacga accctccacc ctccaagatc
     2941 acgtggtagg tcgtgcaatg gtccacgacg acgggcatcc gagagtcgag gtgtcgtcgc
     3001 acctctgcgt ccggagcagg tgcatcgacg ggaacccgag tgacgtaagt gattcgatcc
     3061 cccgagctcg acttcgtcga gcgcattgcc tcttcgacgc ggtcgatatc gcctcgcgat
     3121 ggtctgcccc aaaccgagaa aaccgcgccg tttccgctga tgatctcgca ggtcattgac
     3181 tcgcactcga ttgccgctcg gcccggctca g
//
LOCUS       DASTKS010000337         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1839239, whole genome shotgun sequence.
ACCESSION   DASTKS010000337 DASTKS010000000
VERSION     DASTKS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839239"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFQ35_07025"
     CDS             <1..1206
                     /locus_tag="VFQ35_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9930419.1"
                     /translation="VSSALKSFLSDPASNGLGVGIQYFPLRDPSVPDLCTSDAQCGSH
                     GPCFLKFCQGAAGYVPCSRDSECVTVDKEDVGPCTPLRFCAPLGANGDLVACHDDFEC
                     GARQTCELYNECAGDRSYVCTQPGQACRTQQGQDLGVCTGPESVCVHQSSCNAAQYAA
                     PAAPIAALPGSLAALRASIDAQMPNGQTPTAPALKGAIQAATSWAQSHPDHSVVVLLA
                     TDGLPTECVTNPTTDAMGIAEVRQTASAGVAAAPSVLTFVIGVFGPSDTEARTNLDQI
                     AMAGGTKTAFLVDTSADVSAQFQSALNEIRGSHLSCEYSVPAAPQGQSLDYNKVNVDF
                     TNAGTTQRIAQVKDAAACGSADGWYYDTDPSVANPTRILICPSACERLQNSTMGSVQV
                     ALGCATVVR"
     gene            complement(1213..2952)
                     /locus_tag="VFQ35_07030"
     CDS             complement(1213..2952)
                     /locus_tag="VFQ35_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930420.1"
                     /translation="MTEPLRPEPLVKGEIRPRFVRGRVAPRGVVWFGIRSFWGHLRHF
                     VASGIATEDIDSRDWMTPDDPNELGARVIAALGGDPKASTVLESLGRDLYVDYISDTG
                     DDVSVSSAVAELLVRDLELPDPDHPGETLTVPRGDILLFGGDTAYPVATAQEITNRVL
                     VPFNRAIEKRGPGSPRVLLGIPGNHDWYDGLDGFARMFRRRDDDDDDDLSRPSIVGVS
                     KRMLDHYAEWARELVRGGKVEKPKALVLSGYTPVQNGSYFVLPLTPNIHLFAADRQLK
                     TLDARQRRFLKGWYQSHPEISPWVLLPDPLYAFGRPSPTGAHMVAALGFDFDQRPHFL
                     LAGDLHHYERAADERLLHVVAGGGGAFLHPAPLYEGRLRSNMRWPDSKQSRALLRAVP
                     LKVALGRSGFLPHLALAAFYAPALAFGARFYARLGLIVPAPVLLTLLVGTVYLLIGGV
                     RDKPRAVAPLAYGAGLLTAAIPIVASLAIHWLSGSLHVDTPLWLVGTLTLLAAVFVGA
                     FVFGAYLAVLTRLGLEHTQAFTALDHPGFKHFVRFRVRADGKGVDGWCVGLTDPLRPG
                     ERAVLVDEFQWRI"
     gene            complement(2949..>3159)
                     /locus_tag="VFQ35_07035"
     CDS             complement(2949..>3159)
                     /locus_tag="VFQ35_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9930421.1"
                     /translation="VRDEIIAFFESSYVDAELFVPYSRQRLVSEMHESGRVLGEAYED
                     QGVRVQLRTDPDTLNRLRASLAETA"
BASE COUNT          571 a         1029 c         1076 g          483 t
ORIGIN      
        1 gtgagcagcg cgctcaaatc gttcctgtcc gatccagctt cgaacgggct cggcgtcggg
       61 attcaatatt tccctctgcg cgatccgagc gttccggatt tgtgcacctc ggacgcccaa
      121 tgcggcagcc atgggccgtg ctttttgaag ttctgccagg gagcggcagg ctacgtcccg
      181 tgctcgcgcg actccgagtg cgtcaccgtc gacaaagaag acgtgggccc ttgcacgcca
      241 ttgcgcttct gcgcgccgct cggagcaaac ggcgatctcg tcgcgtgtca cgatgatttc
      301 gagtgcggcg ctcggcagac gtgcgagctc tacaacgaat gcgctggtga tcggagttac
      361 gtgtgcacgc agcctgggca ggcgtgccga acccagcaag ggcaggactt gggcgtttgc
      421 accgggcccg agagcgtttg cgtgcaccaa tcgagctgca acgccgctca gtacgcggcg
      481 ccggcggcgc ccatcgcggc gctgccgggc agcctggccg cgctgcgggc gtcgatcgac
      541 gcgcagatgc cgaacgggca aaccccgacc gcgcctgcgt tgaagggcgc aatccaggcc
      601 gcgacgagct gggcccaatc gcacccggac cattcggtcg tagtgctgtt ggcgacggat
      661 gggttgccca ccgaatgcgt gacgaacccc acgacggacg cgatgggcat cgcggaggtt
      721 aggcaaacgg cgagcgccgg ggtggccgcc gccccgagcg tgctcacgtt cgtcatcggc
      781 gtcttcggcc cgagcgacac cgaagcgcgt acgaacttgg accagatcgc gatggcgggc
      841 ggcacgaaga cggcgttctt ggtggacaca tccgccgacg tcagcgcgca gttccagtcc
      901 gccctgaacg agatccgggg ctcgcacctc tcgtgcgagt actcggtgcc ggcggcgccg
      961 caagggcagt cgctcgacta caacaaggtc aacgtcgact tcacgaacgc tggcaccact
     1021 caacgcatcg cacaggtcaa ggacgcggcc gcgtgtggta gcgcggacgg ctggtactac
     1081 gataccgatc cgagtgtcgc gaacccgacg cgaatcctga tttgcccgtc ggcgtgtgag
     1141 cgcttgcaga actcgacgat gggcagtgtt caagtggcgc tcgggtgcgc gaccgtcgta
     1201 cgctgaacga tttcagatcc gccactggaa ctcgtcgacc agcacggcgc gctcgccggg
     1261 gcgaagtgga tccgtcaggc cgacgcacca cccgtcgacg cctttgccgt cggcgcggac
     1321 gcggaaccga acgaagtgct tgaaacccgg gtggtcgagc gccgtgaacg cctgagtgtg
     1381 ctcgaggccg agacgggtca acaccgcgag gtacgccccg aagacgaacg cgccgacgaa
     1441 cacggccgcg agcaacgtga gggtgccgac cagccagagc ggggtatcca cgtggagaga
     1501 gccggacagc caatgaatcg cgagcgacgc gacgatgggg atggcggccg tgagcaggcc
     1561 cgccccgtag gcgagcgggg ccactgcgcg cggcttgtca cgcacgccgc caatcagcag
     1621 gtagaccgtt ccgacgagca aggtgagcag aacgggtgcg ggaacgatca ggccgagccg
     1681 cgcgtaaaaa cgcgcaccga acgcgagcgc gggcgcgtag aaagccgcga gcgcgagatg
     1741 aggcaaaaag cccgatcgcc cgagcgccac cttgagcggc accgcgcgta agagcgcgcg
     1801 gctctgcttg ctgtcgggcc agcgcatgtt ggagcgcagc cgaccttcgt agagcggcgc
     1861 cgggtgcaag aaggctcctc cgccgcccgc gaccacatgc aatagccgtt cgtcggcggc
     1921 gcgttcgtag tgatgcagat ccccggcgag caagaagtgc ggacgttgat cgaaatcgaa
     1981 gccgagcgcg gccaccatgt gcgcgccggt tgggctcggg cgcccgaagg cgtagagcgg
     2041 atccggtagc agcacccaag gcgagatctc ggggtgcgac tggtaccaac ctttcaagaa
     2101 gcgtcgctgc cgagcatcca acgtcttcag ttgccgatcg gcggcgaaca ggtggatgtt
     2161 cggagtgagc ggcaggacga agtagctgcc gttttgcacc ggcgtgtaac cggacagcac
     2221 gagcgccttc ggcttctcca cctttccgcc gcgtacgagc tcgcgggccc actccgcgta
     2281 gtgatcgagc atgcgcttcg agactccgac gatgctcggt cgcgagaggt cgtcgtcatc
     2341 atcgtcgtcc cgccgacgaa acatgcgcgc gaacccatcg aggccgtcgt accagtcgtg
     2401 gttgccgggg atcccgagca agacgcgcgg cgagccgggg ccgcgctttt cgatcgctcg
     2461 gttgaacggc acgaggacgc ggttcgtgat ttcttgcgcc gtcgcgacgg ggtacgccgt
     2521 gtctccgccg aacaacaaga tgtcgccgcg cggaaccgtg agcgtttctc cggggtgatc
     2581 cggatccggc agctcgaggt cgcgcaccaa gagctctgcc accgcgctgc tcaccgagac
     2641 atcgtcgccg gtgtccgaga tgtagtcgac gtacaggtcg cgcccgagtg actccaacac
     2701 cgtggacgcc ttgggatctc ccccgagcgc ggcgatcact cgcgccccga gctcgttggg
     2761 atcgtccggg gtcatccaat cgcgcgagtc gatgtcttcg gtcgcgatgc cggacgcgac
     2821 gaagtgcctc aagtgtcccc aaaacgagcg gatcccgaac caaaccacgc cgcgcggcgc
     2881 gaccctgccg cgcacgaaac gcggcctgat ctcgcccttc acgagcggct ctgggcgtag
     2941 tggctcggtc acgcggtttc agcgaggctc gcgcgcaggc ggttcaacgt gtccggatcc
     3001 gtgcgcagct gcacccgcac accttggtct tcgtacgcct cgccgaggac gcggccggac
     3061 tcgtgcatct cggagacgag ccgctgccga gagtacggca cgaagagctc cgcatcgacg
     3121 tacgagctct cgaagaaggc gatgatctcg tcgcggact
//
LOCUS       DASTKS010000338         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_276189, whole genome shotgun sequence.
ACCESSION   DASTKS010000338 DASTKS010000000
VERSION     DASTKS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276189"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VFQ35_07040"
     CDS             <1..1703
                     /locus_tag="VFQ35_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930422.1"
                     /translation="PTRDAPFARYLKREPTPSAALLEPATPKPEGVSLREATEATVRR
                     ILKQAQESVRACRDVRAAVRPEVGDVFSIDLSLSTEGKVLRLRVIAGNSAQRDAVLER
                     CVEGVLRSLPFTAYGIAVSVTQTLKVPPLRSARRTVCSPASRLSLPIRRSVWLERSSL
                     DVESYLKAMRSCELTSFAEKREYLLLLLEASPTGAARLTLARELDEAGEPDAAAFVRK
                     EALRRVESFDELEGLSATIFSDEPNVDAEFQKAYSAVGTDEKRLEIVRKFLRIAPHSP
                     VIRRRLFGLLEALGRKDELVSVIDAMREEPIVDAGLLALGASALRRVGLDADGRRAFG
                     ELLERAPEDPWTLAFVGDRLRGERLFDEAQEAYESLQRIVPSDAAVSLRLALAHAGAG
                     RLDVATRLLENVTQTGGRDDDGRLGELASITSAVLLAGAHEQSTLAAERAELLRRLSQ
                     TPLPDVASVLFIQSQPSDEPLQIRVARERSDRDLRAPEFNAEALGLAAVRVERGDGAA
                     RIVLRRATGVEPHRAVRVEIAALLASTDRGDVRLVRREVELPRGAETLEVRWNGEAFL
                     "
     gene            1727..>2009
                     /locus_tag="VFQ35_07045"
     CDS             1727..>2009
                     /locus_tag="VFQ35_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930423.1"
                     /translation="MVEASGLGAPPHQLPYDLLQPPVFDERPQAAPDFRARPIWPCLG
                     PALWMGGVLLWSYVSFGRLVLRQGFPEVAGFLSLAACFALSAYGSLERSL"
BASE COUNT          306 a          661 c          681 g          361 t
ORIGIN      
        1 ccccgacgcg agacgccccg ttcgcgcgct acttgaagcg agagccgacg ccgagcgcgg
       61 cgctgctcga gccagcgacc cccaaaccgg agggcgtttc gctccgtgag gccaccgaag
      121 ccaccgttcg ccgcattctg aagcaagctc aggagtccgt tcgagcctgt cgcgacgtgc
      181 gcgccgccgt gagacccgag gtcggcgacg tgttctcgat tgatctgtct ctttcgaccg
      241 aaggaaaggt cttgcgactg cgcgtaatcg ccggaaattc agcgcagcgc gacgcggtgc
      301 tcgagcgctg cgtcgaaggc gtgctccgca gtctgccgtt caccgcctac ggcatcgcgg
      361 tttcggtgac gcagactttg aaggttccac cgctgcggtc agctcgacgg accgtttgtt
      421 ccccggcgtc gcggctcagc ttgccgattc ggcgctcggt atggctcgaa cggagcagcc
      481 tcgacgtcga gagctacctc aaggcgatgc gcagctgcga actcacgagc ttcgcggaga
      541 agcgcgagta cttgctcctc ctgttggagg cgagtcccac cggagcggct cggttgacgc
      601 tcgcgcgcga gctcgacgaa gccggagagc cggacgcggc cgcgttcgtg cgcaaagagg
      661 cgttacgccg ggtcgagagc ttcgacgagc tggaggggct cagcgccacc atcttctcgg
      721 acgagccgaa cgtcgacgca gagtttcaga aggcgtattc cgcggtgggc accgacgaaa
      781 agcgcctcga gattgttcgc aagtttctgc gcattgcccc tcactcgccc gttatccgac
      841 gccgcctgtt cggcctgttg gaggcgctcg gtcgcaaaga cgagctcgtt tcggtcatcg
      901 atgcgatgcg cgaggagccg atcgtcgatg cgggcctcct cgctctcggc gcatcggcgc
      961 ttcggcgcgt gggcctcgat gcggacggcc ggcgcgcttt cggcgagctc ctcgaacgcg
     1021 cgcccgagga tccgtggact ctggcgttcg tcggcgatcg attgcgtggc gagcgactat
     1081 tcgacgaggc gcaggaagct tacgagagcc tgcaacgcat cgtgccttcc gatgcggcgg
     1141 tcagcttgcg gctggcgctc gcgcatgccg gagccggacg cctggacgtg gcgacacgcc
     1201 tgctcgaaaa cgtaactcag acgggcgggc gcgacgacga tggtcgactc ggtgagctcg
     1261 cctcgatcac gtccgcggtg ttgttggcag gcgcgcacga acaatcgacc ctcgcggcag
     1321 aaagagccga gctcttgcgc cgcttgtccc agacgccgct ccccgacgtc gcgagcgtcc
     1381 tgttcattca aagccagccc tccgacgagc cgctgcagat tcgcgtcgcg cgggagcgca
     1441 gcgatcgcga tctgcgcgcg ccggagttca acgcggaggc tttggggctg gctgccgttc
     1501 gcgtcgagcg cggtgatggc gcggcccgca tcgtgctccg ccgtgcgacc ggcgtcgagc
     1561 cgcaccgagc ggttcgcgtc gagatcgcgg cgctactcgc atcgacggat cgaggcgacg
     1621 ttcggctcgt gcgtcgtgaa gtcgagctcc cgcgcggcgc cgaaaccttg gaagttcgat
     1681 ggaacgggga ggctttcctg tgaccgatcc cgaacaaagt gaggaaatgg tcgaggcttc
     1741 cggccttgga gcgccgccgc accaattgcc ctacgacctc ttgcaaccac ccgtgttcga
     1801 tgaacggcct caggctgcac ctgattttcg agcgcgcccg atttggccct gcctcgggcc
     1861 cgcactgtgg atgggcggcg tgctgctttg gtcgtacgtc tcgttcggtc gcttggttct
     1921 gcgccaaggc ttcccggagg ttgcgggctt cctgagcctc gcggcgtgct tcgccctgag
     1981 cgcgtacgga tcgctcgagc gcagcctca
//
LOCUS       DASTKS010000339         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_54992,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000339 DASTKS010000000
VERSION     DASTKS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54992"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFQ35_07050"
     CDS             complement(<1..648)
                     /locus_tag="VFQ35_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9930424.1"
                     /translation="MTILSALYVAVLATGSAAAGVFCFHLACAPAEAPNLLGIRGLKR
                     VRALQGSPMFASCEGAVRWFGSHIGRLLTPVLRASLDRRLMLAGDVLGLSPGEFVALS
                     LMLSVAAGAFAAGQLAFSTEPEERVMLLVPLAFVMPYARLSSLRDDRLKRVNHGLPPL
                     IDLLVLCLSAGLDLPGALRNVVAQSSNPDDPLSEELGFVLQELRMGKTRKAALTQF"
     gene            complement(645..1508)
                     /locus_tag="VFQ35_07055"
     CDS             complement(645..1508)
                     /locus_tag="VFQ35_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9930425.1"
                     /translation="MTGTGFLGMRTGVCALCFAFALVVLFAIVKDPESLPLRLYSRYV
                     ARLDRMLYNMLLPTCGRWIVVGQALALELIGIACIGFGSAWHYLALSPAFVGPVLYLE
                     RMRVRRIAAIEAQVDGFLLSLANALRTTPSIALALSYCEELTPNPLRSELARALHELR
                     LGSSVDQALLSLGARVRSATLDAGLLSILIGRQVGGDLTKVLETTAATLREATRLQGV
                     LRTKTAEGRAQMLVMALFPVFVVYGFSRAVPDFFDPLLTSATGHILIAAACVLWLSAL
                     IVAHRLLAVHL"
     gene            complement(1505..3181)
                     /locus_tag="VFQ35_07060"
     CDS             complement(1505..3181)
                     /locus_tag="VFQ35_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9930426.1"
                     /translation="MNIEVTVHGNYGRRQFELEAARGPLVVGRQTECNICLEDNHISR
                     YHALLYVGAEGLRIEDRSSNGTLAGDRLLRHETVDVPYGTPIYVGTHTLFLRPAQSRR
                     DMATPTEVPVPGGSVDVSASTPAPTDAPIMPSDRNEDEIALRREIHRQLLQHLDLASL
                     DPKRADDAALRPRVLTALHRIVQAMRDRLPRDTNCDRLVGELADEALGLGPLERLLAA
                     SDVTEIMVIDAHTIYVERRGKLERASERFTDDERVRAVIERIVAPLGRRIDESSPLVD
                     ARLVDGSRANAVIKPLALKGSCITIRKFSKVPLTLEKLYSYNALSPSMGLFLTRSVTA
                     KRNIVISGGTGSGKTTLLNVLSGAIPEEERIVTIEDAAELQLQQPHVVSLETRPANME
                     GTGSYTIRDLVKNALRMRPDRIVVGECRGGEALDMLQAMNTGHDGSLTTTHANSPSEA
                     LTRLETLCLMAGVDLPSRAIREQIASSVQLIVQQSRFSDGSRKVTAIAELCGLRPDGQ
                     FDLQPIYVFRRHGTSADKKVIGQFAATGRLPSFLGEFLVRGLIRPGEPYL"
     gene            complement(3189..4538)
                     /locus_tag="VFQ35_07065"
     CDS             complement(3189..4538)
                     /locus_tag="VFQ35_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET9930427.1"
                     /translation="MNRRAAQVSSALLGLLLYTGPSGAQPPDKASHPEVPDATAVVRQ
                     QQDEVNLSVGENRTLSAVGVRSYSVGAPGIVDVRLTPDNSQFVLTGQQPGSTTLLLLK
                     KDNSQVSYALNVYPRPVERVESELLQLLDGTPGMRVRKVGPRFFIEGGVSTEPELKRI
                     DRIAAQFPGQVESLVVLGGAAAERRINVRIDFFFIEFDRTKSYQIGIGYPGKVGPGAF
                     EASYSFLGGGFTSATASVVNQPLPGLDLAATRGWAKVLKHSTILTANGAEAKFSSGGE
                     QNYVITSGLSASLSQINFGTDVTVLPRFDPVARELEVRIDAQVSDLTPSVAAGTNLPG
                     RAVSKLQTLVSVKLGQSIVLSGIRTRSQRHGVSGLPLLSQIPLLGVLFGSHSDDQQDV
                     EGAVLLVPSVVESVPRSAHEVLDDALAKFEKYSGDLDDAHAWDENSETPAPARSSGH"
     gene            complement(4535..5338)
                     /gene="cpaB"
                     /locus_tag="VFQ35_07070"
     CDS             complement(4535..5338)
                     /gene="cpaB"
                     /locus_tag="VFQ35_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9930428.1"
                     /translation="MNRRALAIAAFLAACFTALLVVYLNRFEREMSGGDKVALLTVVK
                     PVPRGAVITDDMLATRKVPLAYVEDRAVKAAERAKVLGLEASTTLSPQETLLWTDLAI
                     TTEERDLSALVRPGKRAVTVHAGGFNDSPGNALIRPGDYVDVLLTSLDSKDPKDQSTM
                     VLLQRILVLAVGQETSGMRASGDQRGVAPVNQDKVLTLSLSLADAQILALALEKGRLI
                     VAVRNASDPAVQTDMPDVNASMLFTGRARTEAPAVRAAPAGPVAIGGPR"
BASE COUNT         1026 a         1774 c         1712 g          953 t
ORIGIN      
        1 aaactgcgtg agcgcggcct ttcgcgtctt ccccatgcgg agctcctgca gcacgaagcc
       61 gagctcctcg ctgagtgggt cgtccgggtt gctgctctgt gcgaccacgt tgcgtagtgc
      121 gcccggcaag tcgagcccag ccgaaagaca cagcacgagc agatcgatga gcggcggcaa
      181 gccgtggttc actcgcttca agcgatcgtc tcgcaagcta ctgagccgcg cgtaaggcat
      241 gacgaaggcg agaggtacga ggagcatcac gcgctcctcg ggctcggtcg aaaacgccaa
      301 ttgacccgcg gcaaatgcgc cggcagctac ggatagcatc aagctcagcg cgacgaattc
      361 cccgggcgac aagccgagaa cgtcgcccgc gagcatcaag cgccgatcga gactagcccg
      421 cagaaccggc gtgagcaggc gtccgatgtg actaccgaac cagcgtaccg ccccctcaca
      481 actcgcgaac atcgggcttc cttgcagcgc tcgcacgcgc ttcagccctc gaatgccgag
      541 tagattggga gcttctgctg gcgcacacgc gaggtgaaag caaaacacac ccgctgccgc
      601 cgaaccggtg gcgagcaccg cgacgtacaa ggcgctgaga atagtcatag atgcaccgcc
      661 aggagccgat gcgccacgat gagcgcactc agccaaagca cgcacgctgc cgcgatcaag
      721 atgtggccgg tcgcggaggt caggagcggg tcgaagaagt ccggcaccgc gcgactgaat
      781 ccgtacacga cgaacacggg gaacagtgcc atcacgagca tctgtgccct tccctctgcc
      841 gtcttcgtac gcagtacacc ttgcaggcgt gtcgcctcac gcagtgtcgc ggcggtcgtc
      901 tccagtacct tcgtgagatc gccgccaact tgtcggccaa tcaagatcga aagcaacccc
      961 gcatcgagtg tggcgctccg cacccgcgcg ccgaggctga gcaaagcctg gtcgacgcta
     1021 ctacccaacc tgagctcgtg aagggcgcgc gcgagctcgc ttcgcaatgg gttcggcgtc
     1081 agctcttcgc agtaagacaa ggcgagcgct atgctgggag tcgtgcgcag cgcgttcgcg
     1141 agactgagca aaaagccatc cacctgagcc tcgatggcgg cgatgcgccg aacgcgcatc
     1201 cgttcgagat agaggacggg tcccacgaac gcaggcgaca acgcgaggta gtgccacgcc
     1261 gagccgaacc cgatgcacgc gatgccgatg agctcgagcg cgagcgcctg cccgacgacg
     1321 atccaacgac cgcacgtcgg caacagcatg ttgtagagca tgcgatcgag gcgcgccacg
     1381 tagcgcgagt agagccgaag cggcagggac tccggatcct tgacgatcgc gaagagcacc
     1441 accagcgcga acgcgaagca aagagcgcag acgccggtgc gcatccccaa gaagccagtt
     1501 ccggtcatag gtaaggctcc ccggggcgaa tcagcccgcg caccaagaac tcgccgagaa
     1561 agctcggtaa gcgccccgtc gccgcgaatt gtccgatcac ctttttgtcg gcagaggtcc
     1621 cgtgccgcct gaacacgtaa attggctgca gatcgaactg cccgtcgggc ctgagaccgc
     1681 acaactcggc gatcgcagtg acctttcgac tcccgtccga gaagcgcgac tgctggacga
     1741 tgagctgcac gctcgaagcg atctgctctc gaatggcgcg agacggaagg tcgacgccgg
     1801 ccatgagaca caaagtttcg agccgcgtca gcgcctcact cggcgagttc gcgtgggtgg
     1861 tcgtgagcga gccgtcgtgc ccggtattca tcgcctggag catatcgagc gcctcacccc
     1921 cgcgacactc accgactacg atgcgatccg ggcgcatccg cagcgcgttc ttgacgagat
     1981 cgcgaatcgt gtagctaccg gtaccctcca tgttcgcggg acgcgtctcg agcgaaacga
     2041 cgtggggctg ctgcagttgg agctctgctg catcttcgat cgtcacgatg cgctcttcct
     2101 cggggatggc gccggagagc acgttgagca gcgtcgtctt gccgctgccg gtaccgccgg
     2161 aaatcacgat gtttcgcttg gccgtcacgc tgcgcgtcag gaagagcccc atgctcggag
     2221 agagggcgtt gtaagagtag agcttctcga gcgtgagcgg gaccttgctg aacttccgaa
     2281 tcgtgatgca cgaacctttg agcgcgagcg gcttgatcac cgcgttcgcg cgtgatccgt
     2341 cgacgagccg cgcatcgacg aggggagaag attcgtcgat gcggcgcccc agcggggcga
     2401 cgatacgctc gatcaccgcc cgcactcgct cgtcgtccgt gaatcgttca ctcgctcgct
     2461 cgagcttgcc tcgccgctcg acgtagatcg tatgcgcgtc gatcaccatt atttcggtga
     2521 cgtctgacgc cgccagcaag cgctccaaag gaccgagccc gagcgcttcg tccgccagct
     2581 cgcccaccag ccgatcgcag ttggtgtcgc gagggagccg gtcgcgcatg gcctgcacga
     2641 tccggtgaag agccgtgagc acgcgcggcc ggagcgccgc gtcgtcggcg cgcttggggt
     2701 cgagcgatgc caaatccaag tgctggagca gctgtcgatg gatttcgcga cgcagcgcga
     2761 tctcgtcttc gttacggtcg ctcggcatga tcggcgcgtc ggtcggcgcc ggcgtagaag
     2821 cgctgacatc gacgctgcca cccggtacgg ggacctcggt cggcgtcgcc atgtcgcgac
     2881 gtgactgcgc cggccgcaaa aatagcgtat gcgtgccgac atagatcggc gtgccgtagg
     2941 ggacgtcgac cgtttcgtgt cgcaacaggc ggtctcccgc tagcgtaccg ttgctcgagc
     3001 gatcttctat gcgcagccct tccgcgccga cgtagagcag cgcgtgatat cgcgagatat
     3061 ggttgtcctc gaggcagatg ttgcactcgg tttgacgccc gacgacgagc ggaccccgag
     3121 ctgcttcgag ctcgaactga cgtcgaccgt agttcccatg aaccgtaact tcgatgttca
     3181 tgtcagcctc aatgtcccga actgcgcgcg ggcgcaggcg tttcactgtt ctcgtcccag
     3241 gcgtgagcgt cgtcgaggtc gcccgagtac ttttcgaact tcgcgagagc gtcatcgagc
     3301 acttcgtgcg cgctgcgcgg caccgattcg acgacgctcg gaaccagcaa caccgcgcct
     3361 tcgacgtctt gctggtcgtc ggagtgactc ccgaagagta cgcccaaaag tggtatctgg
     3421 ctgagcagcg gcaacccgct caccccatgt cgttgcgagc gtgtgcggat cccggacagc
     3481 acgatggatt gcccgagctt caccgacacc agggtttgca acttcgagac cgcgcgtccg
     3541 ggcaagttgg tgcccgctgc gacggagggc gtcaaatccg aaacctgagc gtcgatgcgc
     3601 acttcgagtt cccgcgcgac cgggtcgaag cgcggcagca ccgtcacgtc ggttccgaaa
     3661 ttgatttggc tgagcgacgc gctgaggccg ctcgtgatta cgtagttttg ctcgccgccg
     3721 ctcgagaact tcgcttccgc gccgtttgcc gtcaaaatcg tggagtgttt gagcaccttg
     3781 gcccagccgc gcgtcgcggc gaggtcgagc cccggtagag gctggttgac gaccgaggcc
     3841 gtcgcgctcg tgaacccacc cccgagaaag ctgtaactag cttcgaacgc cccggggccc
     3901 accttgcccg ggtacccgat cccgatttga tagcttttcg tgcgatcgaa ctcgatgaag
     3961 aagaagtcga tacgcacgtt gatgcgtcgc tcggcggcgg caccgcccag cacgaccagg
     4021 gactcgactt gccctgggaa ctgcgccgca atgcgatcga tgcgcttgag ctcgggctcg
     4081 gtgctcacgc cgccttctat gaagaatcgc ggcccaacct tacgaacgcg catgccgggc
     4141 gtgccgtcca gcaactgcaa gagttcactt tcgacgcgct cgaccggccg cgggtaaacg
     4201 ttgagcgcgt agctcacctg cgagttgtcc ttcttcagca acaaaagggt ggtcgaaccc
     4261 ggttgctgac cagtgagcac gaactgcgaa ttgtccggcg tcaatcgcac gtccacgatt
     4321 cccggtgcac ccaccgaata gctgcgcacc cccaccgcgc tcagagtccg gttttctcca
     4381 accgaaagat tcacctcgtc ttgctgctgc cggaccacgg ccgtcgcgtc cggtacttcc
     4441 gggtgggaag ccttgtcggg aggctgtgca ccgctcggcc ccgtgtagag caacaagccg
     4501 agcaaagcgg aactcacttg ggcggcgcgt cgattcatcg cggtcctccg atggctaccg
     4561 gtcctgcggg cgccgcgcgc acggctggcg cttcggtccg agctcggccg gtgaacaaca
     4621 tgctggcgtt gacgtccggc atgtccgtct gcacggcggg gtcggatgcg ttacgcaccg
     4681 cgacgatcag cctgcccttc tcgagggcaa gcgcgagaat ctgcgcgtcg gccaagctga
     4741 ggctcagcgt gagcaccttg tcttggttga caggggcgac cccgcgctgg tcacccgaag
     4801 ctcgcattcc cgaagtctcc tgaccgaccg cgaggaccaa gatgcgctgc aagagcacca
     4861 tcgtcgattg atctttagga tccttcgagt cgaggctcgt caagagcacg tcgacgtagt
     4921 cgccgggacg aatcagggcg tttcctggtg agtcgttgaa accgcccgcg tgcaccgtaa
     4981 ccgctcgctt gccgggtcgc acgagcgcgc tgaggtcgcg ttcttcagtg gtgattgcga
     5041 gatcggtcca gagcagtgtt tcttgcgggc tgagcgtcgt agaagcctcg agcccgagca
     5101 ccttggcgcg ctcggcggcc ttgactgcgc gatcttccac gtacgcgagc ggcacctttc
     5161 gcgtggccag catgtcatcg gtgatcaccg cgccccgggg cactggcttc accaccgtca
     5221 gcagcgcaac tttgtctccg cccgacatct ccctttcgaa acggttcagg tagacgacca
     5281 gtagggccgt gaaacaagca gccagaaagg cggcgatggc aagtgctcga cgattcacgg
     5341 cgcctcctgt tggacaaccg cgcgaccggt actctcgctt tcgacgatcg cgaccaccgt
     5401 gccactgacg gaactgttcc gcgcgctcac gatcacgctg gcaccgtcct tgaaaaatgc
     5461 gcgag
//
LOCUS       DASTKS010000340         8018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_946282, whole genome shotgun sequence.
ACCESSION   DASTKS010000340 DASTKS010000000
VERSION     DASTKS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946282"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFQ35_07075"
     CDS             complement(<1..274)
                     /locus_tag="VFQ35_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930429.1"
                     /translation="MAKNRIFLPEQLLGSWLSRRSAELSSDELMLFPFRRRFRLVEAA
                     RVMGEVTGAGDRFGISGQVKTLNFLLELGAELFGSSMIIEDSAFRVV"
     gene            complement(410..1774)
                     /locus_tag="VFQ35_07080"
     CDS             complement(410..1774)
                     /locus_tag="VFQ35_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9930430.1"
                     /translation="MRTRLFSAVALLTWSGAAAAQAPGPSPTPDAPAAPPGAGASAPA
                     QGSGTTTSTTTYVPFGVPAPGTDINAGLPSSSRPVNGEQSDNFDFARSGSGPGVVYGG
                     KGASGILPGDRITLVPDVHTVQKGDTLWDLCDQYFHNPWEWPSVWSFNPQIQNPHWIY
                     PGDQLRLRNPNDIGEAGAGRAETRGTDRGGLVNRRVRVPPNTVFLRDQGFIGDPDKDI
                     WGELVGAVEEQMLLAEGNHVYLTLRPGVDLKVGQELTVFREVRQPENVSGARKPKGQI
                     VAVNGTVKIDRWDPKTRIARGVISESVDTIERGAKVGPVGRKFDVIPPKPSARAVQAR
                     VLTSFYPHVYLAQNQVVFLDHGADDGLEAGNTLYVLRKGDTWRASLATNSRMARDRVR
                     MDSPEHVEVETTPLRGDSDKFPEEAVAELRVLRTEKVSAIALVTQSRREVVPGDRAVS
                     LVGR"
     gene            complement(1771..2187)
                     /locus_tag="VFQ35_07085"
     CDS             complement(1771..2187)
                     /locus_tag="VFQ35_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930431.1"
                     /translation="MRAVFICSILVAAISGCSTDRDAMDKRLAALRDDIARLQADNDR
                     LGERVEALEAHPAPAPAPAKESAATSEESRPPLKVVKLVPNAPPAGDSNTGDVAADER
                     VDAPGNRPVIRLYGRESRDERRAQVSSSRQNSAEER"
     gene            2351..3151
                     /gene="pssA"
                     /locus_tag="VFQ35_07090"
     CDS             2351..3151
                     /gene="pssA"
                     /locus_tag="VFQ35_07090"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET9930432.1"
                     /translation="MDLRKTLFVLPNLITLGAVFCGFNAIRLVARDTPTENDVYRAAV
                     LLIFAMLFDMMDGRVARMTKTQSAFGLQLDSLADVISFGVAPSLLVYKWVLYREPVPG
                     ILTAFLYIACAAIRLARFNVLSTSTTGTPVKPGKYIMGLPSPPAAGILISLVVANHGV
                     GGALGAQRYTAILFAVTIVLGLLMISSVRFRSFKDLQLNTGTVLLVLFVVGSSAFVWQ
                     RFRPEFVLVWLLAFYVFIGIVDSLRALGHRLLHGPPAPRDSLPPVQQG"
     gene            3173..4900
                     /locus_tag="VFQ35_07095"
     CDS             3173..4900
                     /locus_tag="VFQ35_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9930433.1"
                     /translation="MAKTPLLAYLQGLTDRALAGCSPSASPLSRRDFLACAVGATLVG
                     CSPESEPPTVPVEARRIAIIGAGLAGLHCAYRLQQSSGGFDVTVYEANLRLGGRVFTG
                     SGLFADKGLSCELGGEFIESEHATMLALADELGVALEDRAAARAGLADTYSVHGVEFS
                     EDKLVAGLTSVAPTLLAALSAADDDDKAFRLLDNTTISRLLETHVPSDKYPELSLLLN
                     VAFRAEFGMETGVLSSLNLWRLFPLDATQITHLTGNARHRLRAADGNRAFIDALLRSV
                     RRVVSDQRLIRVARGSEGDFELTFVSQSLVGTVVHADQIVFALPFSTLRNVDLTGVSL
                     SDTKRSAIANLAYGSSAKLVGTFRGAPWRDRYGKSGDLVSELPAHVAWDGSRGGNQSQ
                     SLLTTLLAGDSGARADRVDVEEAMAAALVNLDATLGTISDEYVDGSAVRMHWFSSPYS
                     LGSSSAYLVMQFGSLYKTEGTPEKVDGTPERKMHFCGEHCSVDFKGSMEGAAETGALV
                     AAEILKDFGVPLPPPLKPLVEMKALLLQPGFSASQSLELGIGARRAFVAQTHREFAGP
                     LLRRVVRAG"
     gene            4958..5953
                     /locus_tag="VFQ35_07100"
     CDS             4958..5953
                     /locus_tag="VFQ35_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9930434.1"
                     /translation="MNRSRTPSADELSGVLEHSWHRFSDLYEPLRPDLYRYCRYLTRS
                     PWDAEDLVQDTFARAFVTLAHLGAPPPNPRAWLLRVASNLWIDQTRRQRPMPPLESGE
                     PARDSRDSREAAGTLLVRLSPQERAAVVLKEVFDLTLEEVAEALATTPGAVKAALSRG
                     RGKLTEDEPVESRRVVPAVLNAFCDAFNARDVDRLTALLLDGAAVEVVGVHSEYGPEA
                     ARRGVLHGMLYGSERMARASEEGGMDPRFIQGVLPDPPRLELRVHRGEPLLLSWYAHE
                     DGEAVRAITRVELEAGRVARLRNYFFTPEFLRDLCGELEVPCKVNGTVGCRDEEG"
     gene            5956..6600
                     /locus_tag="VFQ35_07105"
     CDS             5956..6600
                     /locus_tag="VFQ35_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9930435.1"
                     /translation="MALELYSHPLASFCWKVLIALYDTDTPFEPLLVDLSNSTAAENF
                     KRVWPIAKFPVLRDTARNQLVPESTIIIEYLAQHFPAARDLIPQDPDAALETRLRDRF
                     FDLYVSEPMQKIVLDKLRPPGANDPHGVTQAKNSLEVAYDMLERDMIGRTWATGDHFD
                     MADCSAAPALYYANRVAPIGQRPNVRAYLERLQQRPSFARVLREAEPYFALFPG"
     gene            complement(6965..7720)
                     /locus_tag="VFQ35_07110"
     CDS             complement(6965..7720)
                     /locus_tag="VFQ35_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930436.1"
                     /translation="MVSACGGAAMGGDDSGGAGSAGGSAGPEPASGASSSTAGAATGG
                     SGGIAGAAIVGNGNSAGAAGSSSGPSCGQTLPCGGDVAGTTWTFVDACIEANPGASVV
                     QKLYEEQLGCIGLELRRVWLTNVSGVIRFNADGTYSSAPWDSFNTTFAIPRSCVADAG
                     ATTCDEFAALLPNRAVALGSVQCADAEGGCSCDLLRPPGGSFEQSTYSAETGLFGPKV
                     LTAGYCVEGDLLHVISMYEGRGPIVQDLIAVRQ"
BASE COUNT         1325 a         2817 c         2486 g         1390 t
ORIGIN      
        1 gcacgactcg aaacgccgag tcttcgatga tcatcgagga gccgaagagc tcggcgccga
       61 gttcgagcaa gaagttcaac gtcttcacct gcccgctgat gccgaatcga tcccccgcgc
      121 ccgttacctc tcccatcacc cgcgccgctt cgaccaaacg aaagcggcgg cgaaagggga
      181 acagcatcag ctcgtccgat gaaagctctg ccgagcggcg cgagagccag ctcccgagca
      241 gctgctccgg caaaaagatg cggttcttgg ccatgcaaac gaacgccgac gcggccttat
      301 gggtcagcga cgaagcctag gtcaaggccc cgttggaagc cagtcgccgc gccctccgca
      361 tccccaaatg gcgaatgatt tcgggagcaa acgccgcgaa gggccggcgc tagcggccga
      421 ccagcgacac ggcgcggtcg ccgggcacca cttcgcgccg ggactgcgtc acgagagcga
      481 tcgcgctcac cttctcggtg cgcagcaccc gcagctccgc gaccgcctct tcgggaaact
      541 tgtcggagtc gccgcgcagc ggagtcgttt ccacctcgac gtgctccggc gaatccatgc
      601 gcacacggtc acgcgccatg cgcgagttgg tcgccaggct agcgcgccag gtgtcacctt
      661 tacgcagtac gtacagcgtg tttcccgcct cgagcccgtc gtcggcgccg tgatcgagaa
      721 agacgacctg gttctgcgcg aggtagacgt gaggatagaa gctcgtcagt acccgggcct
      781 gcaccgcgcg ggcgctcggt ttcggcggaa tcacgtcgaa ctttcgtccg acgggcccta
      841 ccttggcgcc gcgctcgatc gtatcgaccg actccgaaat gaccccgcga gcgatgcgcg
      901 tcttcggatc ccaacggtcg attttgaccg tcccgttcac ggcgacgatc tgtcccttgg
      961 gcttgcgcgc cccgctcacg ttctcgggct ggcgtacctc gcgaaacacc gtcagctctt
     1021 ggccgacctt cagatcgacg cccggtcgca gcgtgaggta gacgtggttg ccctcggcta
     1081 ggagcatctg ctcttcgacc gcgccgacga gctctcccca aatgtctttg tcggggtcac
     1141 cgatgaagcc ttggtcgcgc agaaacaccg tgttcggggg cacgcgcaca cggcggttga
     1201 ccagccctcc gcgatccgtc ccgcgcgtct cggcgcgtcc tgcaccggcc tcaccgatgt
     1261 cgttcgggtt gcgcaaacgc agctgatcac ccggataaat ccagtgggga ttctggattt
     1321 gggggttgaa gctccaaacc gaaggccact cccacggatt gtggaagtac tggtcgcaga
     1381 gatcccagag ggtgtcgccc ttctggacgg tgtggacgtc gggcacgagc gtgatgcgat
     1441 ctccaggcaa aatcccagac gcgcccttgc cgccgtagac gacgcccggg ccgcttccgc
     1501 ttcgtgcgaa gtcgaagttg tcgctttgct cgccgttgac gggtcgcgag ctcgaaggca
     1561 agcccgcgtt gatgtcggtg ccgggcgcgg ggaccccgaa tgggacgtac gtggtcgtgg
     1621 acgtcgtggt ccccgaaccc tgagcgggtg ccgaggcgcc tgcgccggga ggagcggccg
     1681 gggcgtcggg cgtcgggctc ggcccgggtg cctgcgcagc tgccgctccc gaccacgtga
     1741 ggagcgccac cgcgctgaag agacgggtcc tcatcgttcc tccgccgaat tttggcgcga
     1801 cgagctgacc tgagcccgcc gttcgtcgcg cgactctcgg ccataaagcc ttatcaccgg
     1861 tcgattcccc ggagcgtcga cgcgctcgtc ggcagctacg tcgccggtgt tggagtcgcc
     1921 ggccgggggc gcgttcggga cgagctttac gaccttcaga ggagggcgcg actcttcgga
     1981 cgtcgccgcg gactctttcg ccggcgcggg ggccggagcc gggtgcgcct cgagggcttc
     2041 gacccgctcg ccgagtcggt cgttgtctgc ctggaggcgc gcaatatcgt cacgcagagc
     2101 cgcaaggcgc ttgtccatcg cgtcgcggtc ggtactacag ccagaaatcg cggcgacgag
     2161 gatggaacaa atgaatacgg cccgcatcgg cgggtatcgt acgaggcctt ttggcgaagg
     2221 gccaaaatct ggcagcgagt ctggaccgca gtggccgctg accctttggg gtccagacct
     2281 ggacgggccg gggccccgcg gttaccctct cgaggtgcgc cttcgccgtt ccagacgccc
     2341 gcgcccgcga gtggaccttc ggaagacgct attcgtcctg ccgaacctga ttacgctggg
     2401 agccgtcttc tgtggcttca atgcgattcg gctagtcgcc cgagacacgc cgaccgaaaa
     2461 tgacgtctac cgcgccgcgg tattgctcat cttcgcgatg ctgttcgaca tgatggacgg
     2521 ccgcgtcgca cgcatgacga agacgcaaag cgccttcggc ctccagctcg actccctcgc
     2581 ggacgtgatc tcattcggcg tcgccccgtc gctgctcgtc tacaagtggg tgctctatcg
     2641 agagcccgtt ccggggatcc tcaccgcgtt cctctacatc gcgtgcgcgg cgatccgcct
     2701 ggcgcgcttc aacgtgctgt cgacctcgac caccggcaca cccgtcaagc ccggcaagta
     2761 catcatgggg ttgccgagcc cgcccgcggc cggcattctg atttcgctcg tcgtcgccaa
     2821 ccacggggtc ggaggcgccc tcggcgcgca gcgctacacg gcgatcttgt tcgccgtcac
     2881 gatcgtgctc gggctgctca tgatttcgag cgtgcgtttc aggtctttca aggacctgca
     2941 gctcaacacg ggcacggtgc tcctcgtgct gttcgtggtg ggctcgagcg ccttcgtttg
     3001 gcagcggttc cgacccgagt tcgtgctggt ttggctgctc gcgttctacg tattcatcgg
     3061 catcgtcgac tcgctgcgcg ccctggggca ccgcctcttg cacgggccgc ccgctccgcg
     3121 cgactcgctt ccaccggtgc agcagggctg agacgggtgc tcggctcttc gcatggcgaa
     3181 gactccactc ctcgcgtacc tgcaaggcct cacggatcgc gcgctcgcgg gctgctctcc
     3241 gagtgcctcg ccgttatcgc gtcgtgactt cttggcgtgc gcggtcgggg ccacgctcgt
     3301 cggctgcagc cccgagtcgg agccgccgac cgtgccagtc gaagctcgac gcatcgcgat
     3361 catcggagct ggcctcgccg ggctgcactg cgcgtatcgc cttcagcaat cgagcggcgg
     3421 cttcgacgtc accgtgtacg aagcgaacct ccgtctcggc gggcgcgtgt tcaccgggag
     3481 cgggctcttc gccgacaagg ggctgagctg cgagctcggc ggcgagttca tcgagagcga
     3541 gcacgcaacg atgctggcgc tcgccgacga gctcggcgtc gcgctcgagg atcgggctgc
     3601 ggcgcgggcg ggcctcgcgg acacctactc ggtgcacggc gtcgagttca gcgaagacaa
     3661 gctcgtcgcg gggctcacca gcgtcgcgcc gacgctgctc gccgcgctga gcgccgcaga
     3721 cgacgacgac aaggcgttcc ggctgctcga caacaccacc atctcgcgcc tgctcgaaac
     3781 gcatgtgccg agtgacaaat accccgagct ctcgctgctc ttgaacgtcg cctttcgggc
     3841 cgaattcggg atggagaccg gggtgctctc gtcgctcaac ttgtggcgac tctttccgct
     3901 cgacgcgacg cagatcacgc atttgacggg gaacgcacgc caccggctcc gggctgccga
     3961 cggcaaccgc gccttcatcg acgcactgct ccgctcggtt cggcgcgtcg tctcggacca
     4021 gcggctcatc cgcgtcgccc gcgggagcga aggcgacttc gagctcacct tcgtgtccca
     4081 gtcgctagtc ggcacggtgg tgcacgccga ccagatcgtg ttcgcgcttc cgttcagcac
     4141 gctgcgcaac gtcgatctca ccggcgtttc gttatcggac accaaacgaa gcgcgatcgc
     4201 gaacttggcg tacggcagca gcgccaagct cgtcggcacc ttcaggggcg cgccgtggcg
     4261 cgaccgctat ggcaagagtg gcgacctcgt gagcgagctt cccgctcacg tcgcctggga
     4321 cggctcgcgc ggcggtaacc aatcgcagag cttgttgacg acgctgctcg cgggggacag
     4381 cggcgcccgc gcggaccggg tcgacgtcga agaggccatg gccgcggcgc tcgtcaacct
     4441 cgacgcgacg ctcggtacga tcagcgacga gtacgtcgac ggcagcgcgg tgcgaatgca
     4501 ttggttctct tcaccgtact cgctcggcag cagctcggcg tacctcgtca tgcagttcgg
     4561 ctcgctctac aagacagagg gcactcccga gaaagtcgat ggtacgccgg agcgcaaaat
     4621 gcacttctgc ggcgagcatt gctcggtgga cttcaagggc agcatggagg gcgcggccga
     4681 aaccggcgcg ctcgtcgccg cagagattct gaaagacttc ggcgtgccct tacctccacc
     4741 gctcaagccc ctcgtcgaga tgaaggcgct tttgctacaa ccgggcttct ccgcgagcca
     4801 atcgctcgag ctcggcatcg gcgcgcgccg cgcattcgtc gcgcaaacgc accgcgagtt
     4861 cgcaggcccc ctcctgcgac gagtcgtccg cgctggctga aacgaagaaa tcgacgcggc
     4921 cccgtatcct ttcgccgcgc ccacccgtct cctcgacatg aatcgcagca gaactccctc
     4981 ggcggacgag ctctctggag tgctcgagca ctcgtggcac cgcttctcgg atttgtacga
     5041 gccgcttcgt cccgacctgt atcgctactg ccgctacctc acgaggagcc cgtgggacgc
     5101 cgaagacctc gtacaggaca ccttcgcgcg cgcgttcgta acccttgcgc acctcggcgc
     5161 gccgccgccc aacccgcgcg cttggttgct gcgcgtggct tcgaaccttt ggatcgatca
     5221 gacccgccgc cagcgcccga tgccgccact cgaaagcggc gaacccgctc gagactcgcg
     5281 cgactcgcgc gaagccgcgg gcacgctgct cgtgcgcctg tcaccccagg agcgggccgc
     5341 ggtcgtcttg aaagaagtct tcgatttgac gctcgaagaa gtcgcggagg cgctcgcgac
     5401 gactcccggc gcggtcaagg cggcgctctc acgcgggcgc ggcaagctca cggaggacga
     5461 accggtcgag tcgcgtcgcg tcgtcccggc cgtgctcaac gccttttgcg acgccttcaa
     5521 cgcgcgcgac gtggatcgcc tcaccgctct cctcctcgat ggcgccgccg tcgaagtcgt
     5581 cggtgtgcac agtgagtatg gccccgaagc cgcgcggcgc ggcgtgcttc acggaatgct
     5641 gtacggcagc gagcgcatgg cccgcgcatc cgaagaaggc ggcatggacc cgcgcttcat
     5701 ccagggcgtc ttgccggacc cgccgcgtct cgagctgcga gtccatcgcg gcgagccgct
     5761 gctgctctcg tggtacgcgc acgaagacgg cgaagcggtg cgcgccatca cgcgcgtcga
     5821 gctcgaagcg ggtcgcgtcg cgcgtctccg caactacttc tttacccccg agttcttgcg
     5881 cgacttgtgc ggcgaactcg aggtcccctg caaggtcaac ggcaccgtcg gctgccgtga
     5941 cgaggaaggt tgaacatggc gctcgagctc tactcccacc cgctcgcttc gttctgctgg
     6001 aaggtgctca tcgctctcta cgacaccgac acccccttcg agccgctcct cgtcgacctc
     6061 tcgaacagca ccgccgccga gaacttcaag cgggtctggc ccatcgccaa attccccgtg
     6121 ctccgagaca ctgcgcgcaa ccaactcgtc cccgagtcga ccatcatcat cgaatacctc
     6181 gcgcagcatt tccccgctgc acgcgacctc attcctcaag atcccgacgc cgcgctagag
     6241 acacgcctcc gcgatcgctt cttcgacctc tacgtctcgg aaccgatgca aaagatcgtg
     6301 ctcgacaaac tccggcctcc aggcgcgaac gacccacacg gtgtcaccca agcgaaaaac
     6361 tcactcgaag tcgcctacga catgctcgag cgcgacatga tcgggcgaac ctgggctacc
     6421 ggcgaccact tcgacatggc cgactgctct gccgcacccg ctctctacta cgcaaaccgt
     6481 gtcgctccga tcggtcagcg tccgaacgtc cgcgcctacc tcgagcgact tcagcagcgt
     6541 ccctcgttcg cgcgcgtcct ccgcgaagca gaaccctact tcgcgctatt ccctggctaa
     6601 gggtcttgaa aaacgccctg ctagaatgct gaccggcacc actgcgcggc tgcgcgtcgt
     6661 gtgtgcctgg gcattggtct gtcgtgacgc agggcgtttt tcaacgccct gctaagggcc
     6721 tgcgccgtca ctctgcagcc cgacgtcaaa ctgcagtacg tgcggtggag gttgaggggg
     6781 cgcccaacga aaagagcgcg caactaagca tcatgcgtct ttagcgcgga cgccgtacga
     6841 ccccgtgcgc gctcccaccg cgcccgcgcc accccgaaaa gacgcgccgg cgtcgctccg
     6901 aaaaggcgcg cccgcctcgc agcggtctcg gctcgctcgc gactgattcg ttcgttcgca
     6961 acggctactg cctcacggca atcaggtctt gcacgatggg ccctctaccc tcatacatcg
     7021 agatcacgtg cagcaagtcg ccttcgacgc aatacccagc cgtgagcact ttggggccga
     7081 acaatccggt ctctgctgaa taggtcgact gctcaaaact tccgcccggc gggcgcaaaa
     7141 ggtcgcatga acaaccgccc tccgcgtccg cgcactggac cgaacccagc gcgacagcgc
     7201 gatttggcaa gagcgccgcg aattcatcac acgtcgtcgc gccggcatcc gcaacgcaac
     7261 tgcgggggat cgcgaaagta gtgttgaatg aatcccacgg cgcagaagag taggtcccat
     7321 ccgcgttgaa acgaatgacc ccgctcacgt tggttagcca gacccgcctg agctcgagcc
     7381 caatgcaacc aagctgctct tcgtacagtt tctggaccac cgatgcaccg ggattcgctt
     7441 cgatacaagc gtcgacgaag gtccaggtgg tacccgccac gtcgcctccg cacggtaacg
     7501 tttgaccgca gcttggtccg ctcgacgagc ccgccgcacc ggcgctgttc ccgtttccga
     7561 cgatcgcggc ccctgcgatt ccgccgcttc ccccagtcgc ggcgcccgcg gtgctgctgc
     7621 ttgcgccaga tgcaggctcg gggcccgcgc tcccgccagc gctccccgca ccgcccgagt
     7681 cgtcgccccc cattgcggcg cccccgcagg ctgaaaccat cgaaagcaaa gcgcttgcag
     7741 tcacaaccgc gaccgctcgc attgaccacc tgtgtgcacc gaactcaccc atcggtctgc
     7801 gaacgccgcc gcccccaaca ttcttcctcg acccgcagct ttttcgcctt gccgccactc
     7861 acgtcggcgt ggcgggtcga atgggctagg gggtgtccca agttcctgcc cgtcgcgaga
     7921 gccaagaaac gcgagcgaga agcgcgcccg aggagtgagc accggaggaa ggctgaagcc
     7981 tttcgaggag cggagcgacg aggccgtgct tcgcagcc
//
LOCUS       DASTKS010000341         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1169836, whole genome shotgun sequence.
ACCESSION   DASTKS010000341 DASTKS010000000
VERSION     DASTKS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169836"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1001)
                     /locus_tag="VFQ35_07115"
     CDS             complement(177..1001)
                     /locus_tag="VFQ35_07115"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9930437.1"
                     /translation="MGLPLLIKALILGIVEGLTEFLPISSTGHLILAGALLQFNDELG
                     KVFEVVIQAGAMLAIIWEYRARFARVTVGLFSDSDARRFVLNLGIAFMPAAVLGLLLA
                     KTIKRTLFHPLPVALAFIIGGLVILWAERRKHVTRIESVERITPLDALKIGLAQALAL
                     IPGTSRSGATIIGGLLFGFSRRTATEFSFFLAVPTLLAASAYDLLKNRALLSVDDGAV
                     FAVGLGAAFISAFLCVRWLLRFIARHDFTWFAWYRIGFGVLVLITAYTGAVDWSQG"
     gene            1495..>2147
                     /locus_tag="VFQ35_07120"
     CDS             1495..>2147
                     /locus_tag="VFQ35_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930438.1"
                     /translation="MFSANCDQCGARNEFPASAQNEGINAGSLIHCPNCGAEVRVRLS
                     ADGSTDEAQPAVASESLGEPVGEAIVPSDDLGDDEKTQVLEASSLPDAAAKRPMPSVD
                     DSEPMTLRRGRNGSPEPIQHTPSAEAEAAAIAELSIEAMEGIEATPESLVPLSERDFI
                     PSERAGRQLPSPPMRPRRAVSATASTDEAATNHAGLVRKAVPHPPRREHHEAVAHDG"
BASE COUNT          456 a          678 c          694 g          319 t
ORIGIN      
        1 ccaatcgagc ggggtctggt tcagtgtggc cgcagcgacc cgaatcaagc gcattaggac
       61 aagactttac acccaagctc gccgaggtcg atgaaacgga tgggaaggag ccgctcccac
      121 cgactttgcc accgccaaac cgcgattgag gcgcggcttt cggcacgaaa agagctctat
      181 ccttgcgacc agtcgaccgc cccggtgtac gcggtgatca gcacgagcac gccgaatccg
      241 atccggtacc aggcgaacca ggtgaagtcg tgacgtgcga tgaagcggag cagccagcgg
      301 acacacagaa aggccgatat gaacgccgca ccgagcccga ccgcgaacac cgcgccgtcg
      361 tcgacgctga ggagcgcgcg attcttcagc aagtcgtatg cgctcgccgc gagcaatgtc
      421 ggaacggcca aaaagaacga gaactcggtc gccgtacgac gagagaaacc gaacaagagc
      481 ccgccgatga tcgtcgcccc cgagcgcgaa gtgccaggga tcaacgcgag cgcttgagcg
      541 agcccgattt tgagcgcgtc gagtggagtg atccgctcca cgctctcgat gcgtgtgacg
      601 tgtttgcgtc gctctgccca aaggatcacg agtccgccga tgatgaacgc gagcgccact
      661 gggagcggat ggaagagcgt ccgcttgatc gtcttggcga gcaacagtcc gagcacggca
      721 gccggcatga acgcgattcc tagattgaga acgaaccgcc gagcgtccga atcactgaac
      781 aggccgacag tcactcgggc aaaccgcgcg cgatactccc agatgatcgc gagcattgct
      841 cccgcttgaa tcacgacctc gaagaccttc ccgagctcgt cgttgaactg caagagcgcc
      901 cccgccagga tgaggtggcc ggtcgaggaa atcggcagaa attccgtcaa gccttctacg
      961 atgccaagaa tgagggcttt gataagtagg gggagtccca tgcggcgcct atgctagtcg
     1021 caggccctga agtcatccgc tcatttcacg cgctgcgccg ccaagcgtca cgcgccgcgc
     1081 tgaccgcgaa gcctggcggg gcgaatcgcg caccgaaaag ctcgccctct tggatggccg
     1141 cgactcgtat tgcggcgcct tctttcaatt gattcggggc cgattttcag gcacagggaa
     1201 cacccctcgt catctgagcg ggtagcgcga gtcgggcccg gcgctcagaa ccgccgatgg
     1261 accttggacg atctcaattc cggaaaggct ggtccaaagt tcggcgcgga agacgagttt
     1321 cgccccgtgc catccgtgcc aaaggcgaag cgtctcgtgg cggctccggc gaaaacttgc
     1381 ggcaatgcgg ctggcccgaa cgaagacacg cggcgtggaa ccccttgcgt cgcgcgacga
     1441 gagcagacag agtgaacgat aacgtttggg gtcggcagac gactggagtg caccatgttt
     1501 agtgccaact gcgatcaatg tggggcgcgc aacgagttcc ccgcgagcgc tcagaacgaa
     1561 gggatcaacg cgggctcgct gattcattgc cctaactgtg gcgccgaagt tcgtgtgcgg
     1621 ctcagcgcgg atgggtcgac cgacgaagct cagccggcgg tcgcgagcga gagcctcggg
     1681 gaaccggtgg gtgaagcgat cgtgccgagc gacgacctcg gcgacgacga gaagacgcag
     1741 gtgctcgagg cgagctcgtt gccggacgca gccgccaagc gccctatgcc ctcggtcgac
     1801 gactcggaac ccatgacgct gcgccgcggt aggaacggct cgccggagcc aattcagcac
     1861 acgccgagcg ccgaggccga agccgctgcc atcgcagagc tctcgataga agcgatggag
     1921 ggtatcgagg cgacgcccga gtccttggtg ccgctcagcg agcgcgactt catcccttcc
     1981 gaaagggcgg gtcggcagct tccctcacct ccaatgcgac cgcgccgcgc cgtgagcgcg
     2041 acagccagca ctgacgaggc cgcgaccaac cacgccgggc tcgtccgcaa agccgtgccg
     2101 catccgccgc ggcgggagca tcacgaagcg gtagcgcacg acggcga
//
LOCUS       DASTKS010000342         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_56024,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000342 DASTKS010000000
VERSION     DASTKS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56024"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2518)
                     /locus_tag="VFQ35_07125"
     CDS             complement(<1..2518)
                     /locus_tag="VFQ35_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930439.1"
                     /translation="MLEPCANARDFEVLGSLEHPNLVPVVDVARSTTEGSSYLVFDLA
                     QPRKTLLEAAENAPVAIQLEWLGELLRALVYLHQRRLGHTPWSTEDILIVGGHVRLLV
                     KSLAEFSDATVQRDLALVAALMSKLAPVQLRAQKLIERLTSPGSKDAITDATTALTEL
                     GRALERALPVETAETRESFLRALPLLGREGVTKTFRSALSRLEEGHGAAFLLEGESGV
                     GKSRVLTQLRRQAIAAGAFVLEGRGEASPSSPYRVFRMPLLRLALRSGSEALDDFELE
                     ALAPLLPDLPTRLGRRIRASAATEELSRDALRGVALTLLRHEREPLVLLLEDMHWSGS
                     ESISLLAELMPLTEHQPLLIVATSRDDQSSLPNAMQRVSLPRLDAEQISEACAALVGP
                     EHPAELATLLFRETEGNAFFLVEALRALAEDAGSLAALKTAPLPNRIFAGGIQALLRR
                     HLGAISERGRPLLRLAAVLGRSVHEDVLSLLEPSADVAAWIAECLSAAVFERVDGGRD
                     ERVRFRHDKLREALLEELSAEERRALHGRVAQALEQTHGEDPEQLAPLARHFGAAGNA
                     EKELFFATRAGEQALRSRAFTEADEFLSRAMELARKIPRPDLRPELCSLRAEVHAQRS
                     EWSQAHAAIDAAFASAGRPLWYRKLGDVAFLLVQLVLHLVRSALYEGARAEQPSSFAN
                     PASDRPPPPKALVRAADVQLNIALTRGNNLLALAASLLALNVAKPGEPTSIRALLLLA
                     LAARVARLRQVTRRYIARANALLPQIADRKELSEALAYCGYYWIGEGKLRRAREYLLR
                     SVAASTSIGYQLPLSWSVGQLSMCASLQGRFVEMLQQAEIC"
BASE COUNT          423 a          840 c          883 g          385 t
ORIGIN      
        1 cgcagatttc ggcttgctga agcatctcga cgaatcggcc ttgcagcgac gcgcacatcg
       61 acaactgccc gacgctccac gagagaggta gttggtagcc gatgctcgtc gacgcggcga
      121 cgctccggag caggtactcg cgagcgcgcc ggagcttgcc ctccccgatc cagtagtagc
      181 cgcagtaggc gagcgcttcg gagagctctt tgcgatccgc gatctggggc aacagggcgt
      241 tcgctcgggc gatgtagcga cgagtgactt gacgcagacg ggcgacgcga gcggccagcg
      301 cgagcaacaa gagcgcgcgg atgctggtcg gttcgcccgg cttcgccacg ttcaacgcca
      361 acaagcttgc ggcgagggcg agcaagttgt tgccgcgcgt gagcgcgatg ttgagttgca
      421 cgtcggcggc gcgcacgagg gctttcggcg gcggcgggcg atcgctcgcc ggattggcga
      481 aagacgaggg ttgctctgcg cgtgctcctt cgtagagcgc gctgcgaacc aggtgcagta
      541 cgagctgaac cagcaggaac gcgacgtcgc cgagctttct gtaccagagc ggccggcccg
      601 cgctcgcgaa ggcggcgtcg atggccgcgt gcgcttgcga ccactccgag cgctgcgcgt
      661 gaacctctgc gcggagcgag cagagctcgg gccgcaaatc gggccgcgga atcttgcggg
      721 cgagctccat ggcgcgcgac aagaactcgt cggcttcggt gaaagcgcgg ctacggagcg
      781 cctgctcccc cgcccgggtc gcgaaaaaga gctccttttc ggcgttccct gcggcgccga
      841 aatggcgggc gagaggcgcg agctgctccg gatcctcacc gtgggtttgc tcgagcgcct
      901 gggcgactct cccgtggagt gcgcgacgct cttccgcgct cagctcctcg agcagggcct
      961 cacgcagctt gtcgtgccga aagcgaaccc gttcgtcgcg tccgccgtcg acccgctcga
     1021 agacggccgc gctgaggcat tcggcgatcc acgccgcgac gtcagcgctc ggctcgagga
     1081 gggagagcac gtcttcgtgc acgctgcggc cgagcaccgc ggcgaggcgc aagagcggac
     1141 ggccgcgctc ggatatggcg cccaagtgac gacgcaagag agcctggatt ccgccggcga
     1201 agatccggtt cggcagcgga gccgtcttca acgctgcgag cgagcccgcg tcttcggcga
     1261 gcgctcgcag cgcttcgacc aggaagaagg cgttgccctc cgtttcgcga aatagcagcg
     1321 ttgcgagctc ggcggggtgc tctgggccta cgagggccgc gcacgcctcg ctgatttgct
     1381 ccgcgtcgag ccggggcaag gagacgcgct gcatcgcgtt cggcagcgag ctctggtcgt
     1441 cacggctggt cgcgacgatg agcaggggct ggtgctccgt gagcggcatg agctctgcga
     1501 gcagcgagat gctctcggaa cccgaccagt gcatgtcttc gagcaagagc accaacggct
     1561 cgcgctcgtg ccgcagcaag gtgagcgcca cgccgcgcaa agcgtcgcgc gagagctctt
     1621 ccgtcgcggc agacgcacgg atacggcgcc cgagccgcgt cggtaaatcg gggagcagcg
     1681 gtgcgagtgc ctcgagctcg aagtcgtcga gcgcctccga accgctgcgc agcgcgaggc
     1741 gcaagagcgg catgcgaaag acccgatacg ggctcgaagg gctagcctcg ccacggccct
     1801 cgagcacgaa cgcaccagcg gcgatcgcct gcctgcgcag ctgcgtgagc acgcgcgact
     1861 taccgacgcc gctctctccc tcgaggagga aggcagcgcc gtgcccttcc tcgagcctgc
     1921 tcagcgcgga ccgaaacgtt ttggtcacgc cctcgcgtcc gagcagcggc aacgcgcgca
     1981 aaaagctctc gcgcgtctcg gcggtttcga cgggcaaagc ccgctcgaga gcgcggccga
     2041 gctcggtcaa cgccgtggtc gcgtccgtga tcgcgtcttt cgaccccggg ctggtaagcc
     2101 gctcgatgag cttttgcgct cgcagctgca ccggcgcgag cttgctcatg agcgcagcga
     2161 cgagagcgag gtcgcgctgc acggtcgcgt cggagaactc cgccaggctc ttgaccaaga
     2221 gccgcacgtg tccgccgacg atcagaatgt cttcggtcga ccacggcgtg tgccccaagc
     2281 gccgctgatg cagatagacg agcgcgcgca agagctcgcc gagccattcg agctgaatcg
     2341 cgaccggcgc gttctcggcc gcttcgagca gagtctttcg cggctgcgcc aggtcgaaga
     2401 ccaaatagct cgagccctcg gtcgtcgaac gcgcgacgtc caccaccggc accaggttcg
     2461 ggtgctcgag cgagccgagc acttcgaagt cgcgagcgtt cgcgcacggt tcgagcatca
     2521 cttgctgatc c
//
LOCUS       DASTKS010000343         4174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_723751, whole genome shotgun sequence.
ACCESSION   DASTKS010000343 DASTKS010000000
VERSION     DASTKS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723751"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFQ35_07130"
     CDS             <1..892
                     /locus_tag="VFQ35_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET9930440.1"
                     /translation="NPPSSGASVPNHQSLSDLGNGTVRDNVTCLTWEKANPTAQGTWQ
                     DAYDRCAKLAQDGYAGFDDWRLPTRVELASISDVTLGSTGFPKVFGVTSGYYVTGSFW
                     YKTILTANESKPEDRVWGYGTNGFTSNAIVRSNTNNVTRCVRGNGKGEAADAYAVEPP
                     NHYTVVGTGADATVTDNYTGLTWQQTFSAARMPWSEAASYCASQTTGGVSGWRLPTLN
                     ELASTVNEALVAPAVNRTVFPKTNGACDETGWYWAAEASKVGGTAWGISYCDGYTGWN
                     AGSSASAWNYFTEGFVRCVR"
     gene            complement(906..1952)
                     /locus_tag="VFQ35_07135"
     CDS             complement(906..1952)
                     /locus_tag="VFQ35_07135"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HET9930441.1"
                     /translation="MDRDVRARLEELLRGARRLADDADPIGREARARLTRSSGLSREN
                     VEWALREALETSPSDAELEALVESVATTPRSHVLLSANVFVAAHRAIALALAASPEVY
                     VRPSRREPEMTELLAEATPGLFRRVDELTPSAGDQLFAYGRAETLDQVARALPEGVLL
                     STHGPGFGVVVLSEPLEDDERLATALSVDITAFDQRGCLSPRVALLEAPRERALAFAK
                     LLAASLTARQNEVPLGALGEQERAEIARYRDTLLYTGELYPAGEGYVGVAAPDAPLEL
                     APVGRNLHVLAVDDATALLEPVQHAITTFSHAGPEERRERLAAILPHARAAKLGRMQT
                     PLFDGPVDRRVKAR"
     gene            complement(1953..3071)
                     /locus_tag="VFQ35_07140"
     CDS             complement(1953..3071)
                     /locus_tag="VFQ35_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HET9930442.1"
                     /translation="MSVVDESEALHARVRAFAAAAPQREAFEALALEIAEYQARYNPG
                     FARLVRARGGGLASVDAIPAVPSDAFRLTRVATYPQELDVARFATSGTTGSERGIHPF
                     RTTATYEELATMFGRRALVAGNTRYTAVALAPRLDAPPSSSLGFMMALFMRDFDGRPL
                     TQPSEPFDFRASDRWLLSEAGPDVRGLERAAQIATNRREPLLLLATSFALVALLDALA
                     SRALPLPPGSVVMQTGGYKGRTREVPAAELRASLARCFDLREDAIVSEYGMTELSSQL
                     YEGTLPGASLSGQRGVYLEPAWLRVTPVHPVTLEPVADGEPGLARFVDLANVDSAVAM
                     ITRDLVRRRDGGVELLGRAEGAPPRGCSLAVEALLAGK"
     gene            complement(3068..>4174)
                     /locus_tag="VFQ35_07145"
     CDS             complement(3068..>4174)
                     /locus_tag="VFQ35_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9930443.1"
                     /translation="AGGFGALLLGHCEPRVSFVVAQQSKRLLQALGDVYPSDRKLELG
                     ARLARLYSGDARVIVGQSGSDAVSAALKTAQLFSGRSGVLAFAGAYHGLGYGPLAACG
                     LRSSYRDPFAEQLNQHVRFVEYPSEATELASCLDGARRALSTERIGAVLVEPILGRGG
                     VVVPPRGFLSGLSELCRESGALLVVDEIWTGLGRAGKLLFSAGEAHADLVCLGKGLGG
                     GLPISAVIGRSDVMQAWQRPREVVHTSTFAGNPLAAATAVATLDILENDGLVARAEET
                     GARFRDELAAMLTRKFPSIRVRGAGLMLGVDLGARPGAGSRLQEDLLARGYITSTGGV
                     GREVLVLTPPLNIDEGLLAAFSVELLAALTELAP"
BASE COUNT          757 a         1398 c         1395 g          624 t
ORIGIN      
        1 gaacccgccg agcagcggcg cgagcgtgcc gaaccaccag agcttgagcg atctcggtaa
       61 cggaacggtg cgcgacaacg tgacctgcct cacgtgggag aaggcgaacc cgactgcgca
      121 agggacgtgg caagacgcct acgaccgctg cgccaagctc gcgcaagacg gctatgcagg
      181 tttcgacgat tggcgtctgc cgacgcgagt ggagctcgct tcgatcagcg acgtcacgct
      241 cggctcgacg ggttttccga aagtgttcgg cgtcaccagt ggctactacg tcacgggatc
      301 gttttggtac aagacgatcc tcactgcgaa cgaatcaaag ccggaagata gagtgtgggg
      361 gtacggcacc aatggcttca cgagcaacgc catcgttcgt tcgaatacga acaacgtcac
      421 gcgatgcgtg cgcggtaacg gcaaaggcga agccgccgac gcgtatgcgg tggaacctcc
      481 caaccactac accgtcgtcg gtaccggcgc cgacgcgacc gtgaccgaca actatactgg
      541 gctcacgtgg cagcaaacgt tctccgcggc gcgcatgccc tggtcagagg ccgcgagcta
      601 ctgcgcttcg caaacgacgg gcggtgtatc aggctggcgc ctgcccactt tgaacgagct
      661 cgcctccacc gtgaacgagg cgttggtagc gcccgcggtg aaccgcaccg tgtttccgaa
      721 gaccaacggc gcttgcgatg aaaccggctg gtattgggcg gcagaagcct ccaaggttgg
      781 cggcaccgcc tggggaataa gctattgcga cggctacacg gggtggaacg ctggttcgag
      841 cgcgtccgcg tggaactact tcaccgaggg cttcgtccgc tgcgtgcgct gaagcgactc
      901 gttcactagc gagccttgac ccggcggtcc acggggccgt cgaacagcgg agtttgcatg
      961 cgaccgagct ttgccgcgcg tgcatgcggt aaaatcgcag cgaggcgctc tcgccgctct
     1021 tcggggccgg cgtgcgaaaa ggtggtgatg gcgtgctgca ccggttcgag caacgctgtc
     1081 gcgtcgtcga cggcgagcac gtggaggttg cgcccaactg gcgcaagctc gagtggggcg
     1141 tccggggcgg caacgcctac gtacccttcg ccggccggat agagctctcc agtatagagc
     1201 agcgtgtcac ggtatcgtgc gatttcggct cgctcttgct ctccgagcgc accgagcggc
     1261 acctcgttct gtcgtgcagt gagactcgcg gcgagcagtt tggcaaacgc gagcgcacgc
     1321 tcgcgaggag cctcgagcaa cgccacgcga gggctcaggc agccccgctg atcgaaagcg
     1381 gtgatgtcca cgctcagcgc ggtggcgagt cgctcgtcgt cctcgagcgg ctcactcagg
     1441 acgaccacgc cgaaaccggg cccgtgcgtc gagaggagca caccttctgg cagcgcgcga
     1501 gcgacttggt ccaacgtttc ggcgcgaccg tacgcgaaca gctgatctcc tgcgctcggc
     1561 gtgagctcgt cgacacgtcg gaatagcccc ggggtcgctt ccgccaagag ctcggtcatc
     1621 tcgggctcgc gtcgcgaggg ccgcacgtac acttcggggg aagcggcgag ggcgagcgcg
     1681 atggcgcggt gagccgcgac gaacacgttc gcgctgagca gcacgtggga tcggggtgtg
     1741 gtggcaacgc tctctacgag cgcttcgagc tctgcgtcac tcggcgaggt ctcgagcgct
     1801 tcgcggagcg cccattcgac gttctccctc gacaagccgc tcgagcgggt gaggcgcgcc
     1861 cgagcttcgc gaccgatcgg atctgcgtcg tccgctagcc tgcgcgcgcc gcgcagcaac
     1921 tcttcgaggc gagcgcgaac gtcgcgatcc attcacttcc ccgcgagcag cgcttcgacc
     1981 gcgagcgagc acccacgcgg cggagcgccc tcggctctac ccaaaagctc gacgccgccg
     2041 tcgcgccgcc gcaccaaatc gcgagtgatc atggcgacgg ccgagtcgac gttggcgaga
     2101 tcgacgaaac gagcaagacc gggctctccg tctgcgacgg gttcgagcgt caccgggtgt
     2161 acgggggtga cgcggagcca cgccggttcg aggtacacac cgcgttggcc gctgagcgaa
     2221 gcgcctggga gcgttccttc gtaaagctga ctcgagagct ccgtcatccc gtattcggag
     2281 acaatcgcgt cctctcgcaa atcgaagcag cgggcgagcg aagcgcggag ctccgccgcg
     2341 ggcacttctc gcgttcggcc cttgtagccg ccggtctgca tgacgacgct gcctggggga
     2401 agcggcagtg cgcggctcgc gagcgcgtcc agcaaggcaa ccagcgcgaa cgacgtcgcg
     2461 aggagtagga gcggttcacg ccgattggtg gcgatttgcg cggcccgctc gaggccacgc
     2521 acgtcgggcc ctgcttccga cagcagccag cggtcgctcg cgcggaagtc gaacggctcg
     2581 ctcggttggg tgagcggacg tccgtcgaaa tcgcgcatga acagcgccat catgaacccg
     2641 agcgaggagc tcggcggcgc atccaagcgc ggggcgagcg cgaccgccgt gtaccgcgtg
     2701 ttccccgcca cgagcgccct ccgcccgaac atcgtcgcga gctcctcgta ggttgcggtg
     2761 gttctgaacg gatgaatccc gcgttcgctc cccgtcgtgc cgctcgtcgc aaaccgcgcg
     2821 acgtcgagct cttgcgggta cgtcgcgacg cgcgtgagcc gaaaagcgtc actcggtacg
     2881 gccggaatgg cgtcgacgga agcgagccct ccgccgcgcg cgcgcacgag ccgagcgaag
     2941 ccaggattgt aacgcgcctg atactccgca atctcgaggg cgagcgcctc gaaagcctct
     3001 ctttgcggag ctgcggcggc gaacgcgcgg acccgagcgt gtagcgcctc ggactcgtcg
     3061 acgacgctca tggggcgagc tccgtgaggg cagcaagaag ctcgaccgaa aacgccgcca
     3121 agagcccttc gtcgatgttc aggggaggag tcagcaccaa cacttcgcgg ccgactcctc
     3181 ccgtcgacgt gatgtagccg cgagccagca agtcttcctg caaacgcgaa cccgcgccgg
     3241 ggcgagcgcc gagatcgacc cccaacatca atcccgcacc acgcaccctg atactcggaa
     3301 acttgcgagt cagcatcgct gcgagctcgt cgcgaaagcg cgcgccggtc tcctcggccc
     3361 gcgcaacgag gccgtcattc tcgaggatat cgagcgtcgc aaccgcggtg gcggccgcga
     3421 gcggattgcc ggcgaacgtc gaggtgtgca cgacctcgcg cgggcgctgc caagcttgca
     3481 tgacgtcgct ccgcccgatg acggcgctga tgggcaggcc acctcccagc ccctttccca
     3541 ggcagaccag atccgcgtga gcctcgccgg ctgaaaacag caacttgccg gcacgaccga
     3601 gcccggtcca aatctcgtcg acgacgagca aggcgccgct ctcacgacag agctccgaga
     3661 gcccgctcag aaacccgcgc ggtggaacca cgaccccgcc gcggccgagg atgggttcga
     3721 ccagcaccgc gccgatgcgc tccgtggaga gagcccgacg tgcgccatcg aggcaggagg
     3781 cgagctccgt cgcttcgctc ggatactcga cgaaccgaac gtgctggttc aactgctccg
     3841 caaacggatc gcgatagctc gagcggagcc cacacgccgc gagcggacca tagccgagcc
     3901 cgtgataggc acctgcgaat gcgagcacgc cactgcgccc gctgaagagc tgagccgtct
     3961 tcaacgccgc gctcacggcg tcggagcccg attgcccgac gattacgcgc gcgtctccgg
     4021 agtacaatcg cgcgagacgc gcgccgagct ctagcttgcg gtcggatggg tagacgtcgc
     4081 cgagcgcttg cagcaatcgc ttggactgct gcgcaacgac gaaagagacg cgcggctcac
     4141 agtgcccgag cagcagcgcc ccgaagccgc ccgc
//
LOCUS       DASTKS010000344         5842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1618104, whole genome shotgun sequence.
ACCESSION   DASTKS010000344 DASTKS010000000
VERSION     DASTKS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618104"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFQ35_07150"
     CDS             <1..893
                     /locus_tag="VFQ35_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9930444.1"
                     /translation="FQDLARSLRAQLEWAADCGTLGIPSVPLVESEPAIPRPVSSPLR
                     AEPSAESRVQVASPSAESRAQVASPSVEGRAPVSSPTTAAPVRLEPPLPRAPIAVPAD
                     AEGRRARLTVLADEVRGCTRCVLHAERTQTVFARGTGSAGLCFVGEGPGADEDAQGYP
                     FVGAAGQLLDRMIVAMGLTRDEVYVCNIVKCRPPKNRKPEPDEMAACKPYLEEQLELV
                     QPQVLVALGATAVQGLLGTTEGITRLRGKWKLYRGRIPVMPTFHPAYLLRTPGAKREV
                     WEDLQAVLRQMGRVLPERRR"
     gene            complement(1052..2812)
                     /locus_tag="VFQ35_07155"
     CDS             complement(1052..2812)
                     /locus_tag="VFQ35_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930445.1"
                     /translation="MHRQFLRLLSLCLVTLFALPALGQYTRDAAANRKIDEAINTHYL
                     ATDFDKAEAVLTGTIKACDDKCSPGTLAKAWMYVGIVRGSGRNDQAGAKEAFTTALAT
                     DPNVKLDSGLATPETQATFANSGGAGAVETPTSPAGKAEKAAPAAPAGEAPEGNGLTC
                     TPSVTEVETRRPIPVQCKIDEEVTAVELRYKAFGSETWKTLKMEHRGDSFRAQVPCEA
                     TQTAGVLKLYVRARNATGADMGNWGSKSEPVEISLVEQTAMDPPSYDDAEAPARCAAK
                     EICPPDFPGCDSGGGSKESGEQCGNTSECKSGLLCMDGTCENAPSCSSNSDCSVGTCQ
                     DGKCAVEGGESVSAPAKKWWVGAHFALDFAFVGGQDLCLGDSQTTGNWACYWSNSTEK
                     PYDPAQMNTTAVGNVGSGAVLGTKRVLLSLDRVLTPNVTAGLRVGFAFGGGPPAGKQV
                     EWAGKPTATPNENGEDAIKSVINAGKNFFPIHAEARVGYWFGKNVFAKKGLRPYVHAG
                     GGVAQVDAKVIVHTADKYGDTYTADAWKKMGTGFVTLGGGAVYAFTRNVGLQLNLNAM
                     IMLGASGFVLEPSGGLVFGL"
     gene            complement(2915..4033)
                     /locus_tag="VFQ35_07160"
     CDS             complement(2915..4033)
                     /locus_tag="VFQ35_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9930446.1"
                     /translation="MMGRRSRFAVASLPLALALSCAEAPRLRGQIAGLEKVVEQAERN
                     GAVRCAPRELAIARSQLKFAAIELDQGFVSKASAHMFRAEPNARAALFLSPPEYCATR
                     AFVETPGDRDGDGYPDNVDKCPEQPENFNNFQDEDGCPEDPDTDGDGITDSIDACVLE
                     AEDKDGYLDEDGCPEVDNDLDGLLDTADKCPLEAEDLDSYEDADGCPDPDNDKDTVPD
                     VKDQCPNEIGSPTEEPIGCPKNQLVIVTDCEVKITQQVHFEFNKDKIRPESFGLLDAV
                     VDVLAKNPAIKVEIQGHTDNVGSARYNKDLSNRRAASVRKYLVSHGVSPDRLTSMGYG
                     MERPIVENTTDRNRQLNRRVQFVRTEGVKEGCPKTGTP"
     gene            complement(4030..4335)
                     /locus_tag="VFQ35_07165"
     CDS             complement(4030..4335)
                     /locus_tag="VFQ35_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9930447.1"
                     /translation="MATLLRLTLLVGLGIAATGCGNFVYAIQVNSASAKLEEARSLGA
                     EQYAAYEYYMAQQHLEKAQSEASEADYGDAIELSSSAEEYADKAITLAREAHRGAGR"
     gene            complement(5016..5654)
                     /gene="lexA"
                     /locus_tag="VFQ35_07170"
     CDS             complement(5016..5654)
                     /gene="lexA"
                     /locus_tag="VFQ35_07170"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9930448.1"
                     /translation="MQGLTKRQEQTLDFIRRSIEERGYPPTLREIGEYMGIRSTNGVN
                     DHLRALERKGYLRREDMKSRALKLVEDAAPPAPRASEEELVEVRVLGRVAAGLPLFAE
                     ENVIDTIRVDRMLVRGGRDVFGLRVTGDSMIDAGILSGDYIFVKRQATAERGDIVVAL
                     IGDEATVKYYYPERDYVRFQPANAQMAPILVRATDFKSTMLLGKVVGVYRRL"
BASE COUNT          962 a         1882 c         1903 g         1095 t
ORIGIN      
        1 agtttcagga tctggcgcgc tcgctacgcg cgcagctgga atgggccgcc gactgcggaa
       61 cgctgggcat tcccagcgtt cccttggtcg aaagcgagcc agccatcccc cgaccggtgt
      121 cgtctccgct tcgcgcagag ccgagcgctg aaagccgagt gcaggtcgcg tcaccgagcg
      181 ctgaaagccg agcgcaggtt gcgtcaccga gcgtcgaagg gcgagccccg gtctcgtcgc
      241 ccaccaccgc cgctccagtg cgcctcgagc cgcctttgcc gcgcgcgccg atcgccgtgc
      301 cagcagatgc cgagggtcga cgagctcggc tcacggtgct cgcagacgag gtgcgcggct
      361 gcacgcgatg tgttctgcac gccgagcgca cgcaaacggt gttcgcccgg ggtaccggga
      421 gcgccggact ttgcttcgtg ggtgaggggc ccggcgcgga cgaagacgcg cagggctatc
      481 cgttcgtcgg cgcggcgggg cagttactcg atcgcatgat cgtggcgatg gggctcacgc
      541 gggacgaggt gtacgtttgc aacatcgtca agtgcaggcc gcccaagaac cgaaagcctg
      601 agcccgacga gatggctgcc tgcaagccgt acctggaaga gcagctcgag ctcgtgcagc
      661 cgcaggtgct ggtggcgctt ggtgcaaccg cggtccaagg gttgctcgga acgactgagg
      721 gaattacccg gctacgcggg aagtggaagc tctatcgcgg ccgcatcccc gtgatgccga
      781 cgttccatcc ggcgtaccta cttcgaacgc cgggcgcaaa gcgcgaggtc tgggaagatc
      841 tgcaggcggt gctgcggcaa atgggccgcg tactccccga gcgccgccgt taagaccggc
      901 gcttccgagc gattgctcgc gcgagcgctt gcgtctcagc tgcgcaaccg cgccgcgatt
      961 tgtccaagcg atgggagtct ccgcgagccg gggcggtgca cccgccccca gccgacgcgg
     1021 aactctcgaa ccgagcgcgt gccgtggctt tctaaaggcc gaagaccagg ccgcccgacg
     1081 gctccaacac gaagcccgac gcgccgagca tgatcatggc gttcaggttc agttgcaggc
     1141 ccacgttgcg agtgaaggcg tagacggcgc cgccaccgag ggtgacgaag ccggtgccca
     1201 tcttcttcca agcgtccgcc gtgtacgtgt cgccgtactt gtcggcggtg tgcacgatga
     1261 ctttggcatc tacctgcgcg acgccgccgc ccgcgtgaac gtaggggcga agacctttct
     1321 tcgcgaacac gttcttgccg aaccagtaac caacgcgcgc ctcggcgtgg atggggaaga
     1381 agttctttcc cgcgttgatg acgctcttga tggcatcttc gccgttttcg ttcggcgttg
     1441 cggtaggctt acccgcccac tcgacttgct tgcccgcggg cggaccgccg ccgaaagcaa
     1501 agcccacgcg gagcccggcg gtgacgttcg gcgtgagaac ccgatcgagg ctcaagagca
     1561 cgcgcttcgt accgagcacg gcaccgctgc cgacgttgcc gacggcggtc gtgttcatct
     1621 gagcggggtc gtacggcttt tcagtgctgt tgctccagta gcaagcccag ttgccggttg
     1681 tctggctgtc gcccaagcaa aggtcttgtc cgccgacgaa cgcgaaatcg agcgcgaagt
     1741 gagcgccgac ccaccacttc ttagcgggcg cggagaccga ctcgccgcct tcgacggcgc
     1801 acttaccgtc ctggcaagtt cccaccgagc aatcggagtt gctgctgcac gagggcgcgt
     1861 tctcgcaagt gccatccatg cagagcaggc ccgacttgca ttccgaagtg ttgccgcact
     1921 gctcgccaga ttctttcgag ccgccgccgg agtcgcagcc cgggaagtcg ggcgggcaaa
     1981 tctctttcgc tgcacagcgg gcgggggcct cggcgtcgtc gtagctcggc gggtccatcg
     2041 ccgtctgctc gacgagggag atctcgacgg gctcgctctt gctgccccag ttccccatgt
     2101 cggcgccggt cgcgttgcga gcgcgcacgt agagcttcag tacgcccgcg gtctgggttg
     2161 cttcgcaggg aacttgggca cggaagctgt cgccccgatg ctccatcttc agcgtcttcc
     2221 acgtctcgct tccgaacgcc ttgtaacgga gctcgaccgc ggtcacctct tcgtcgatct
     2281 tgcactggac cggaatcggt cggcgcgtct cgacctcggt cactgacggc gtgcaggtga
     2341 gaccgttgcc ttccggcgct tcgccggcgg gcgcggccgg cgcggccttt tcggctttgc
     2401 ctgccggaga ggtcggcgtc tctactgcgc cagcgccgcc cgagttcgcg aacgtcgcct
     2461 gagtctccgg cgtcgcgagc cccgagtcga gcttcacgtt cggatccgtc gcgagcgcgg
     2521 tggtgaacgc ttccttcgcg cccgcttggt cgttacggcc gctgccgcgc acgatgccga
     2581 cgtacatcca ggctttcgcg agcgtcccag ggctgcactt gtcgtcgcaa gccttgatgg
     2641 ttcccgtgag aaccgcttcc gctttgtcga agtcggtcgc gaggtagtga gtattgatcg
     2701 cctcgtcgat cttgcgattg gcggctgcgt cgcgcgtgta ctgacccaat gccggaaggg
     2761 cgaacagggt caccaagcaa aggctcagca ggcgaaggaa ctgtcggtgc acgtctcgat
     2821 ctccgggtag gcggactgac gctcgcttag cgcagatccg ggaaaatgtg tcgtggcttg
     2881 gtgacaatgg ctcaccctcc acggttagac cgagctaggg cgtgccggtc ttggggcaac
     2941 cttccttgac cccctcggtc cgaacgaact ggacgcgtcg gttgagttga cgattccggt
     3001 cggtcgtatt ctcgacgatg gggcgctcca tgccgtagcc catcgaagtg aggcgatcgg
     3061 gtgagacgcc gtgcgagacg aggtacttgc gcaccgacgc agcgcggcga ttgctcaaat
     3121 ctttgttgta ccgggccgag ccgacgttgt cggtgtgacc ctggatttcg accttgatgg
     3181 ccggattctt cgcgaggacg tcgaccaccg cgtccagcaa gccgaagctt tcggggcgga
     3241 tcttgtcctt gttgaactcg aagtggacct gctgggtgat cttcacctcg cagtcggtca
     3301 cgatgacgag ctgattcttg gggcagccga tgggctcttc ggtgggcgac ccgatctcgt
     3361 tcggacactg gtccttgacg tcgggcacgg tgtccttgtc gttgtccgga tccgggcagc
     3421 cgtcggcgtc ctcgtagctg tccaggtctt cggcctcgag cgggcatttg tcggcggtgt
     3481 cgagcaggcc gtcgagatcg ttgtcgacct ccgggcagcc gtcttcgtcg aggtagccgt
     3541 ccttgtcctc cgcctcgaga acacaggcgt cgatcgagtc ggtgattccg tccccgtcgg
     3601 tgtccggatc ttccgggcag ccgtcttcgt cctggaagtt attgaaattt tcgggctgct
     3661 ccgggcactt gtcgacgttg tcggggtagc cgtctccgtc ccgatccccg ggggtttcga
     3721 cgaaggcgcg cgtggcgcag tactcgggcg gcgacagaaa caaggccgcg cgtgcgttgg
     3781 gctcggcgcg gaacatgtgc gccgacgcct tcgaaacgaa gccttggtcg agctcgatag
     3841 ccgcaaactt gagctggctt cgggcgatcg cgagctctcg cggagcgcag cggacggcgc
     3901 cgttgcgctc ggcctgctcg accacctttt cgagccccgc gatctgcccg cgcagccgag
     3961 gtgcctcggc gcagctcagc gcgagcgcga gggggagcga ggcgacagcg aagcgagagc
     4021 gacggcccat catcgacccg caccgcgatg ggcttctctc gccagcgtga ttgccttgtc
     4081 ggcgtattcc tcggccgaac tcgatagttc gatggcgtct ccgtagtcag cctcggatgc
     4141 ttcgctctgc gctttctcga ggtgctgctg agccatgtag tattcgtagg cggcgtactg
     4201 ctcagcgccg agcgagcgcg cctcctcgag cttggccgaa gcagagttca cctgaatcgc
     4261 gtaaacgaaa ttgccgcagc cggtggccgc aatgcccagc ccaaccagca atgtgagccg
     4321 cagcagcgtc gccaacgctg gactgcttcg atgaatcagt gcgggtgcgg gctcgcgctg
     4381 cgcttccgaa tagagtgcgc cagagcccag gaaatttctg gggcctcgcc ccgtcggtac
     4441 tccagagatc tctggggcgt tcgccggatt caggacacta ggctcgcttc gcaccggatg
     4501 ctcgctcaca agaacatccc gccaccttcg tgctcggtga cccgggaatt tcgctgcgac
     4561 ggtagcaact ggctcagttt ccagtcaacg acgatttcgc caacgctggg cctcggcgga
     4621 tcgcagaatc ccccattgaa gaaatattca gcggcgccag taggctcgca ccgagctcgg
     4681 cggagcagtt ccttccgacg ccgaagcgta aaaggtcagt cgcggccgct cggcctaggg
     4741 cagcctcgaa cttccgcccg tggtgagcca agaacaagga gcgttcgcgg ttctagatgc
     4801 cagctccgag ctcaggcccg acgagcgcga acggcgagcg gcgacgcgaa ccgcgcatgc
     4861 gtcggctcca agagcgagct gaagcagggc gataggcgca ctgtctcgaa ctgcgctgac
     4921 cgaggcgcag ttcttcagca tcgcggatgg ccctcgtgtg caccgcgccc aattgtcgtt
     4981 cgggcctgac aacccgacgg ggagcgtgct cgcgcctaga gacgacgata cacgccgacc
     5041 accttgccga gcagcatggt cgacttgaag tcggttgcgc gcaccaggat cggcgccatt
     5101 tgcgcgttgg ccggctggaa ccgaacgtag tcgcgctcgg ggtaatagta ctttaccgtc
     5161 gcctcgtccc cgatgagcgc tacgacgatg tcgccgcgct cagccgtagc ctgccgcttc
     5221 acgaagatgt agtcgccgct cagaatgccg gcgtcgatca tcgagtcgcc ggtgacccgt
     5281 aggccgaaaa cgtcgcgccc gccgcgcacg agcatgcgat cgacccggat ggtatcgatg
     5341 acgttctctt ctgcgaacag gggcagaccg gccgcgactc gaccgagcac ccgcacttcg
     5401 acgagctctt cttcgctcgc gcgcggcgcg gggggcgcag cgtcttcgac gagcttgagc
     5461 gctcggctct tcatgtcttc gcggcgcagg tagcctttgc gctcgagcgc gcgcaggtga
     5521 tcgttcacgc cattggtgga gcgaatcccc atgtactcgc cgatctcacg caacgtgggc
     5581 gggtagcccc gctcctcgat cgagcggcga atgaaatcca atgtctgctc ttggcgcttc
     5641 gtcaggccct gcataggcgc gaaggatact gaacgggtgt aaagacgtca aattctgctc
     5701 cgcctgatca aacggagacc taaaaaaatc attcaaatcg aacagttaac agcatgtacc
     5761 caggatgctc cccaacccct gtggatccaa aaatcactct tcagggtgac gatcgcgctc
     5821 gatcgctcga tcgcgcccga gc
//
LOCUS       DASTKS010000345         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1841116, whole genome shotgun sequence.
ACCESSION   DASTKS010000345 DASTKS010000000
VERSION     DASTKS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841116"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFQ35_07175"
     CDS             complement(<1..905)
                     /locus_tag="VFQ35_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9930449.1"
                     /translation="MNFSALFIRRAVATTLLSLAIVLAGAWAFTLLPVSPLPRVEFPT
                     INVSASLPGASPEIMASAVATPLERRFGRIAGVQEITSTSSLGTTNISLQFSLDRNVE
                     SAARDVQAAIAAAGGELPANLPSRPTYRKVNPADAPIMVVSLSSATLPLAQVFDAANT
                     VLAQKLSRVSGVGQVFVGGGQQPAVRVQVDPEALAGIGIGLEDVRTALAESTANSPKG
                     TFGTGLTSGAIRANDQLFGAAAFRSLIIGTNQGAPVRLVDVAKVFDDVENNRVAAWSN
                     GTRSVLLIIRRQPGANIIETNERIRA"
     gene            complement(902..>2495)
                     /locus_tag="VFQ35_07180"
     CDS             complement(902..>2495)
                     /locus_tag="VFQ35_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9930450.1"
                     /translation="SERVFDAAVALYDRGLKWVLRHQRTTLLSAVATLVLTGLLAVWI
                     PKGFFPSQDTGMLLGVTEAGADVSFSRMMELEERASDVVRQDPDVDNVVSYIGSDGTN
                     PTTNSGRLSITLKPRSQRSANASEIAARLRPKLAEVAGITVHLQALQDLQIDTRLSRT
                     QYQYTLEDADPDELATWSKRILSELKKRPELADVASDQQEGGLALSLTVDRDSAGRLG
                     ITPQTIDDTLYDAFGQRQISTIFTQLNQYRVILEAPPAMKNDPRALDRLYVRSKSGDL
                     VPLSSFTRYEEQSAPLIVSHQGQFPSATLSFNLAHGASLGAAVDAIHEVERDIALPPS
                     VRADFTGAARAFGESLSSAPVLLLAAVITVYIVLGVLYESYIHPITILSSLPSAGVGA
                     LLALIVCGQEFSIIALIGVVLLIGIVKKNAIMMIDFALEAERDEKLSPEEAIHKACLL
                     RFRPIMMTTLAALLGGVPLAFGSGTGSELRRPLGISIVGGLLISQVLTLYTTPVIYLY
                     MTRAAHALSRLRHRPEALAPQP"
BASE COUNT          438 a          767 c          871 g          419 t
ORIGIN      
        1 gcccggattc gctcgttggt ctcgatgatg ttcgcgccgg gctgacgtcg gatgatgagc
       61 aacacggagc gagtgccatt gctccacgcc gccacgcggt tgttctcgac gtcgtcgaac
      121 accttggcga cgtcgaccag ccgcaccggc gctccttggt tcgtcccgat gatgagtgag
      181 cgaaaagcgg ccgcgccgaa cagttggtcg ttcgcgcgga tcgcgccgct cgtgagaccg
      241 gtgccaaagg tgcccttcgg cgagttcgcc gtgctctccg cgagcgccgt tcggacgtct
      301 tcgagcccga tgccgatacc ggccagcgct tctggatcca cctgcactcg gaccgccggt
      361 tgttgaccac cgcccacgaa gacctggccg acgcccgaca cgcgagagag cttttgcgcg
      421 aggacggtgt tggctgcgtc gaacacttgt gcgagcggca aggtcgccga gctcaacgat
      481 acgaccatga tcggcgcgtc ggcagggttc accttgcggt acgtggggcg cgacggcagg
      541 ttcgccggga gctcgccgcc ggcagcagcg attgccgctt ggacgtcgcg cgccgccgac
      601 tcgacgtttc ggtccagtga gaattgaagc gagatgttgg tcgtgccgag tgagctcgtc
      661 gacgtgattt cctgaacccc ggcaatgcgc ccgaacctgc gctcgagcgg ggtcgcgacc
      721 gccgacgcca tgatctcggg gctagctccc ggcagcgacg cgctcacgtt gatggtgggg
      781 aactcgacgc gcggtaacgg cgaaaccggc agcaaagtga aagcccacgc tccggccagc
      841 acgatcgcga gcgacagcag agtcgtcgcg acggcgcggc ggatgaacag tgccgagaag
      901 ttcatggctg cggagcgagc gcttcgggac ggtgccgcaa gcgcgaaagg gcgtgggcgg
      961 cgcgcgtcat gtaaaggtag atgaccggcg tcgtatagag cgtgagcacc tgcgaaatga
     1021 ggagcccgcc aacgatggag atgccgagcg ggcgtcgcag ctctgaccca gtgccggagc
     1081 cgaaggcgag cggtacgccg ccgaggagcg cggcgagcgt cgtcatcatg atgggtcgaa
     1141 agcgcaagag acaggctttg tgaatggcct cctcgggcga cagcttttcg tcgcgctctg
     1201 cttcgagtgc aaagtcgatc atcatgatcg cgttcttttt gacgatgccg atgagtagga
     1261 cgaccccgat gagggcgatg atgctgaact cctgcccgca gacgatgagc gcgagcaggg
     1321 ctcccacccc cgccgatggc agcgacgaca agatcgtgat cgggtggatg tagctctcgt
     1381 aaagcacacc gagcacgatg tagacggtga tcacggccgc gagtagcagc accggcgcgc
     1441 tcgagagcga ctcgccgaac gcgcgcgcgg ccccggtgaa gtcagcgcgc accgaggggg
     1501 gaagggcaat gtcacgctcg acctcgtgaa tcgcgtcgac ggctgcgccg agcgacgccc
     1561 cgtgcgccaa gttgaaggac agcgtcgccg acgggaattg accctgatgg ctgacgatga
     1621 gcggggcgct ctgctcttcg tagcgggtga aggacgagag cgggacgagg tcgccgctct
     1681 tcgagcgcac gtagagccga tccaacgcgc gcgggtcgtt cttcatcgca ggcggcgcct
     1741 cgaggatgac gcggtattga ttcagctgcg tgaaaatggt cgagatttgc cgctgtccga
     1801 aggcgtcgta cagcgtatcg tcgatggttt gtggcgtgat gccgaggcgc ccggcgctgt
     1861 cacggtcgac cgtgagcgaa agcgccaagc cgccctcctg ctggtcgctc gcgacgtccg
     1921 cgagctccgg gcgcttcttc aactcgctga gaatgcgctt ggaccacgtt gcaagctcgt
     1981 ccggatccgc gtcctcgagc gtgtactggt actgggtccg gctgaggcgc gtatcgattt
     2041 gaagatcttg cagcgcttgc aagtgcaccg tgatgccagc gacctcggcg agcttcgggc
     2101 ggaggcgcgc ggcgatctca ctcgcgttcg cgctgcgttg cgagcgcggc ttcagcgtga
     2161 tcgagagccg cccgctgttc gtcgtcgggt tcgtcccatc ggagccgatg tacgagacga
     2221 cgttgtcgac gtcggggtcc tggcgcacga cgtcgctcgc gcgctcttcg agctccatca
     2281 tccgcgagaa ggagacgtcg gcgcctgcct cggtgactcc gagcagcatg cccgtatcct
     2341 gagacgggaa gaagcctttg gggatccaca ccgcgagcag gccggtgagc acgagggtcg
     2401 caacggccga gagcagcgtg gtgcgctggt gtcggagcac ccatttcagc ccgcgatcat
     2461 aaagagcgac cgccgcgtcg aacacgcgct ccgag
//
LOCUS       DASTKS010000346         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_278065, whole genome shotgun sequence.
ACCESSION   DASTKS010000346 DASTKS010000000
VERSION     DASTKS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278065"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFQ35_07185"
     CDS             complement(<1..294)
                     /locus_tag="VFQ35_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930451.1"
                     /translation="MTRLVVRYYSPTHCAQYVLEAQCGARFRLARDGELRLPESEEDL
                     VLVLGSEGNEWYVQPVRGTLRTSAGRVVASRIAVIPRFQFTLGAATLVLDRAID"
     gene            complement(291..1409)
                     /locus_tag="VFQ35_07190"
     CDS             complement(291..1409)
                     /locus_tag="VFQ35_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9930452.1"
                     /translation="MIPTRVYEETLLQFFAPVRTYLTDPSVSEVMINGPDQIFIERNG
                     LLELTTAKFSSEAAMLAALRNAAQFVGKHVDDLNPILEGRLPDGSRLQGLLRPIATTG
                     PYVSIRRFFQSKLTLAHLVETGSLSPLAAETLTTLVKSKLNVIVAGGTGTGKTSLLNL
                     LTSFIPQGERVGVIEDSKEIQVQHPHVFQLEARKPDEEGRGEVTIRDLFRASLRMRPD
                     RVIIGEIRGGEALDIVQAMVSGHGGCMGTLHASHPRDTLTRLETMAMMSDIEMPLVAL
                     RLQIASGVNLIVQVGREPSGRRVITHITEVLGYDAERGKYEISDVFRREYTGSGSSLE
                     SQLVPTGAPLRCEEAVIEHGHQLPLALRDAMALHRARS"
     gene            complement(1406..2056)
                     /locus_tag="VFQ35_07195"
     CDS             complement(1406..2056)
                     /locus_tag="VFQ35_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930453.1"
                     /translation="MTTRHIRLLALVFPLALVACGASGNGGRIEAPKEPTAAERQQAA
                     TELHRLGISHYRNGDSLRAEEYLASALAAGGVPDVILPDLMRISVNGHRYQAAIRYFE
                     DYRSELAGPNRAELELVAGVLYLGVEQPELARASFEKSLELRPGNARAELMLGQVLHD
                     ELQDYAVADTHFRAYLELEPEGKGAAIARAGLLKSPAEVNERKARPQKLAPRRAVR"
     gene            complement(2053..>2852)
                     /locus_tag="VFQ35_07200"
     CDS             complement(2053..>2852)
                     /locus_tag="VFQ35_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9930454.1"
                     /translation="SSALIGSGGHCDVRLPPDHVAVEQLLLEIQPGGLFAEARARTPQ
                     VTVDGVPFQRGRVLPERDFKVGPITIHAELAQAKVKKQSEQRVRPRVLVLAALVLPIG
                     AFMAFNPQNAEADTDYKSAPPLFDSTPPACAEQSEAAATSAVESWLRDAEGRHERAPF
                     SPEDAVQAVHLYRKSASCLEKFGRAAPASELRDGADGLAHRLERDFHVHRVRLARALA
                     ADDVDAARHEVALLRGYLPRGQSDFALWLDYLDRKLGLRSNEKGRKK"
BASE COUNT          469 a          966 c          925 g          492 t
ORIGIN      
        1 gtcgatggcg cgatccaaga cgagcgttgc cgcgcccaac gtgaactgga acctcgggat
       61 gaccgcgatg cggctcgcca ccacccgccc cgcagaggtg cgcaatgtcc cgcgaaccgg
      121 ctgcacgtac cactcgttcc cctccgagcc gaggacgagg acgaggtctt cttcgctttc
      181 aggcaggcgg agctcaccgt cgcgcgcgag gcgaaagcgc gccccgcact gcgcctcgag
      241 cacgtactga gcgcagtgcg tgggcgagta gtacctgacg actagccgag tcatgatcgc
      301 gctcggtgaa gagccatcgc gtcccgaagc gccaacggca gctggtggcc gtgctcgatc
      361 accgcttctt cgcaacgcaa gggagcaccg gtcgggacca attggctctc gagcgagctg
      421 ccggagccag tgtactcacg ccggaagacg tcggaaattt cgtacttccc gcgctcggcg
      481 tcgtacccca gcacctcggt gatgtgagtt attaccctgc gcccgctcgg ctcgcgcccg
      541 acttgaacga tgaggtttac gcccgatgca atctgcagac gcaacgcgac gagcggcatc
      601 tcgatgtcgc tcatcatcgc catcgtttcg aggcgagtca gcgtgtcgcg cgggtggctc
      661 gcgtgcagcg tccccataca ccctccgtgg ccgctcacca tggcctgcac gatgtcgagc
      721 gcctctccgc cgcgaatctc gccgatgatc acgcgatccg gtcgcatgcg caggctcgcg
      781 cggaacaggt ctcgaatcgt cacttcaccc cgaccctctt cgtcgggctt gcgcgcctcg
      841 agctgaaaca cgtgcgggtg ctgcacctga atctccttcg agtcttcgat cacgccgacg
      901 cgctcgcctt ggggaatgaa cgacgtgagc aggttgagga gcgacgtctt gccggtgccc
      961 gtaccaccgg ccacgatcac gttgagcttg ctcttcacca gcgtcgtgag agtctcggcc
     1021 gcgagcgggc tcagcgagcc ggtttcgacg aggtgcgcga gcgtgagctt cgattggaag
     1081 aagcgacgga tcgagacgta gggaccggtg gtggcgatcg gccgcagtag gccttgcagg
     1141 cgcgagccat ccgggaggcg tccctccaga atcgggttca gatcgtcgac gtgcttaccg
     1201 acgaactgcg ccgcgttgcg gagcgcggcc agcatcgccg cttcgctcga gaacttcgcg
     1261 gtcgtgagct cgagcaggcc gttgcgctcg atgaagatct ggtccggacc gttgatcatc
     1321 acctccgaga ccgacggatc cgtcaaatac gtgcgcaccg gcgcaaagaa ctgcagcagc
     1381 gtctcttcgt aaacacgcgt cggaatcatc gcactgccct ccgtggcgca agcttttgcg
     1441 gccttgcctt cctctcattc acctccgcgg gcgacttcaa gaggcccgcg cgcgcgatcg
     1501 ccgcgccttt accctccggc tcgagctcca agtaggcgcg aaagtgcgtg tccgcgacgg
     1561 cgtaatcttg cagctcgtcg tgcagcacct gaccgagcat gagctccgcg cgcgcgttcc
     1621 ccggacgcag ctcgagcgac ttttcgaacg acgcgcgcgc gagctccggc tgttcgacgc
     1681 ccaggtagag cacgcccgcc acgagctcga gttcggcccg attgggaccc gcgagctccg
     1741 accgatagtc ttcgaagtag cggatcgccg cctggtagcg gtgcccattg accgagatgc
     1801 gcatcaggtc gggcaagatc acgtccggaa cgccgcccgc tgcgagagcg ctcgccaagt
     1861 actcttcggc gcgaagcgag tcgccgttgc gatagtgcga gatcccgagg cggtgcagct
     1921 ccgtcgccgc ttgctgacgc tccgcggcgg ttggctcttt cggcgcttcg attcgaccgc
     1981 cgttgccgga agcgccacaa gcgacgagcg cgagcggaaa gacgagcgcc aataggcgga
     2041 tatgccgagt cgtcatttct ttcgtccttt ctcgttcgag cgcagaccga gctttcggtc
     2101 gaggtagtcg agccacaggg cgaagtcgct ttgcccgcgc ggcaagtaac cgcgaagcag
     2161 cgcgacttcg tgccgtgccg cgtcgacgtc atcggcggcg agggcacgcg ccaagcgcac
     2221 ccgatgcacg tggaagtccc gctccaggcg gtgcgcgagc ccgtccgcgc cgtcgcggag
     2281 ctcgctcgcc ggtgccgcgc gcccgaactt ttcgagacag ctcgccgact tgcggtacag
     2341 gtgcaccgct tgtacggcgt cttcggggct gaacggcgcg cgctcgtggc ggccctccgc
     2401 gtcgcgaagc catgactcga ccgcgctcgt cgcggcggct tcactctgct cggcgcaggc
     2461 ggggggcgtc gagtcgaaca gcggcggcgc gctcttgtaa tcggtgtcag cttccgcgtt
     2521 ttgcgggttg aatgccatga atgcgccaat cggcaggacc agcgccgcga gcacgagcac
     2581 gcgggggcgt acacgctgct cgctctgctt ctttaccttc gcctgcgcga gctcggcgtg
     2641 aatcgtgatg gggccgactt tgaaatcgcg ctccggcagc acgcgcccgc gctggaacgg
     2701 aactccgtcg acggtcactt gcggcgtgcg cgcccgcgct tcggcaaaca acccgcccgg
     2761 ctggatttcg aggagcaact gctcgaccgc gacgtgatca gggggcaaac gcacgtcgca
     2821 gtggccgccc gaaccgatca gcgcgctgct cg
//
LOCUS       DASTKS010000347         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1171865, whole genome shotgun sequence.
ACCESSION   DASTKS010000347 DASTKS010000000
VERSION     DASTKS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171865"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VFQ35_07205"
     CDS             <1..1718
                     /locus_tag="VFQ35_07205"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HET9930455.1"
                     /translation="KKESKSRGNYTPPEIILDRVRMDFAVLESAPNVEAKAGCVLVAR
                     EDLEGMDLEDGAVVSVYRPGAEGAARQLKLVAQKKLPRRVALLTAEDRAALGLKPASS
                     GLDTMVAEVPRLPAEMRVSVEDKSKPAPGADAFRWFFYASSPPWSNTRHSLTNVRLAQ
                     KDFLIKLRNVYSFFTIYANIDGWSPTDKAHAGRPLRERNLLDRWMLGELALAVEKVRS
                     SLESYMPYDAAACLVDLAESLSNWYVRRSRARFWGPGLEADKCDAYATLYEVLSTLSR
                     LIAPFVPFFAEELYQNLVVRPGLPEARQSVHLDQFPIADLSLVDHKLSEETRVVREII
                     SLGLRVRTTARLKVRQPLSRADVVFADSAWQTRLQSYADLIKEELNVHEVRFLQGRDE
                     GAVSFRLKPNFRALGPRLGKEVQAVKKALETADGARLYAELAEKSVARIEVNGNVLEL
                     NPSELEVVVEAAPGFAAESGKVGVVVLHTVLTDELVDEGILREILSRIQAARKDGGLG
                     FTDRIVLDIDGTERVVRVARAGEAEIKQECLASGIRFAARGGKAETHDIGDEQLVLSL
                     EKVAG"
     gene            1715..2305
                     /locus_tag="VFQ35_07210"
     CDS             1715..2305
                     /locus_tag="VFQ35_07210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9930456.1"
                     /translation="MTSTAPFVHPSAVVEDGAELGEGVKVWHFCHVMAGARLGAGSVL
                     GQNCFVAASVRVGVGARVQNNVSLYDGVELGDHVFVGPSVVFTNVRNPRAEFPRKAEY
                     RRTVVERGVSIGANATILPGVRLGAYAFVGAGAVVTKDVAAFELVVGTPARRIGWMSR
                     AGERLEFRGYEARCPLNGELYRLEGHRVELARADDG"
     gene            complement(2374..2703)
                     /gene="cyaY"
                     /locus_tag="VFQ35_07215"
     CDS             complement(2374..2703)
                     /gene="cyaY"
                     /locus_tag="VFQ35_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HET9930457.1"
                     /translation="MIDEREYDERAQPELRALIEALDALEADDFDAELSADILAIEFP
                     DGGKYVVNSHRAARQIWMAAERSAWHFDYDASSGQWRAGKTGDELWSTLARALGKKLG
                     RAISLQK"
     gene            3027..>3269
                     /locus_tag="VFQ35_07220"
     CDS             3027..>3269
                     /locus_tag="VFQ35_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930458.1"
                     /translation="MSRLLWVLLLWVLLLRILLLLLGILLLRVLLLRITALLRRRLIF
                     ALATDGDARKRCDGKHTHGSNAQERSHHETLSLFPLS"
BASE COUNT          552 a         1015 c         1100 g          602 t
ORIGIN      
        1 gcaagaagga gagcaagagc cgcggtaact acactccgcc ggaaatcatc ctcgaccgag
       61 ttcgcatgga ctttgctgtg ctcgagtcgg ccccgaacgt cgaggcgaaa gctggctgtg
      121 tgctggtggc gcgcgaagac ctcgagggta tggatctcga ggatggcgcg gtggtcagcg
      181 tgtaccgccc gggagccgaa ggcgccgctc gtcagttgaa actcgtggcc cagaagaagc
      241 tgccccgtcg cgtggccctg ctcaccgcgg aagatcgcgc ggcgctcggt ttgaagcccg
      301 cgtcgtccgg cctcgatacg atggtcgcag aagttccacg cttgcccgcc gaaatgcgcg
      361 tcagcgtgga agacaagagc aagccggcgc caggggcgga cgctttccgc tggttcttct
      421 acgcctcgtc gccgccctgg agcaacacgc gccacagcct tacgaatgtg cggctcgctc
      481 agaaagactt tctgatcaag ctgcgcaacg tctattcgtt cttcaccatc tacgcgaaca
      541 tcgatggttg gagcccgacc gacaaggcgc acgccgggcg tcccttgcga gagcgcaact
      601 tgctcgatcg ctggatgctc ggtgagctcg cgctcgcggt agaaaaggtg cgctcgtcgc
      661 tcgagagcta catgccgtac gacgcggcgg cgtgcctcgt cgatctcgcc gagagcctct
      721 cgaattggta cgtgcgccgg agccgcgctc gcttttgggg ccccgggctc gaagccgaca
      781 agtgcgacgc ctacgccacg ctctacgaag tgctctcgac tctctctcgc ctgatcgcgc
      841 cgttcgtgcc gtttttcgcg gaagagctgt accaaaacct ggtcgtgcgc cctggccttc
      901 ccgaagctcg ccagagcgtg cacctcgacc aattcccaat tgcggatctc tctctcgtcg
      961 atcacaagct ctccgaagag acgcgcgtcg tacgcgagat catcagcttg ggcttgcgcg
     1021 ttcgcacgac ggctcgcctc aaggtgcgcc aaccgctgtc gcgcgcggac gtcgtgttcg
     1081 cggacagcgc ttggcagact cggctccaaa gctacgccga cctcatcaaa gaagagctca
     1141 acgttcacga ggtgcgcttt ctgcaagggc gtgacgaagg cgccgtttcg ttccgcttga
     1201 agcccaattt ccgcgcgctc gggccgcgtt tgggcaaaga ggtccaggcg gtgaagaagg
     1261 cgctcgaaac ggccgacggc gcgcgcctct acgcggagct cgccgaaaag tcggtcgcgc
     1321 gcatcgaggt gaacggcaac gtgctcgaac tcaatccgag cgagctcgag gtcgtggtgg
     1381 aggccgcgcc gggcttcgcc gccgagagcg gcaaggtagg cgtcgtcgtg ctgcacaccg
     1441 tgctcaccga cgagctcgtc gacgagggca tcctgcggga gatcctgagc cgcatccagg
     1501 ccgcccgcaa agacggcggg ctcggcttca cggatcgcat cgtgctcgac atcgacggca
     1561 ctgagcgcgt cgtgcgcgtc gctcgcgccg gcgaggcgga gatcaagcaa gagtgtctcg
     1621 cgagcggcat tcgcttcgcc gcgcgtggtg gcaaagcgga gacccacgac atcggcgacg
     1681 agcagctcgt gctttcgctc gagaaggtcg ccggttgacg tccacggcgc ccttcgttca
     1741 tccgtcggcc gtcgtggaag acggcgcgga gctcggagaa ggcgtcaaag tgtggcactt
     1801 ctgtcacgtg atggccggcg cccgcctcgg cgccggcagc gtgctcgggc aaaactgctt
     1861 cgtcgccgcg agcgtgcgcg tcggagtcgg ggcgcgcgtt caaaataacg tgagcctcta
     1921 cgatggcgtc gagctcggcg accacgtctt cgtcgggcct tcggtggtgt tcaccaacgt
     1981 tcgaaacccg cgagccgagt ttcctcgcaa ggcagagtac cggcgcaccg tcgtcgagcg
     2041 cggcgtgagc atcggcgcca acgcgaccat tttgcccggg gtgcgactcg gcgcttatgc
     2101 attcgtcggc gcgggtgcgg tggtaaccaa ggatgtcgcc gctttcgagc tcgtagtcgg
     2161 aacgccggcg cgacgcatcg gctggatgag tcgcgccggt gaacggctcg agtttcgagg
     2221 ctacgaggcg cgctgcccgt tgaacggcga gctctaccgg ctcgagggtc accgcgtaga
     2281 gctcgcccgg gccgacgacg ggtagggaag cttccgcgcg cgcgcgccat gggtgaatag
     2341 gcgaattcgc gccgaagcac ggcgcgctgc cctttacttc tggagcgaaa tggcgcggcc
     2401 gagcttctta ccgagtgcgc gagccaaagt gctccaaagt tcgtcgccgg tcttcccggc
     2461 ccgccattgc ccgctggaag catcgtagtc gaaatgccaa gcggagcgct ccgccgccat
     2521 ccaaatctgg cgggcggcgc gatgcgagtt cacgacgtac ttgccaccgt ctggaaactc
     2581 aatcgccaaa atgtccgcgg acaattcggc gtcgaagtcg tctgcctcga gagcatccag
     2641 cgcttcgatc agcgcgcgaa gctcgggttg tgctcgttcg tcgtattcgc gttcgtcaat
     2701 catggcgggc gtttcgttct agcttttggc gcgagccctt cgactcgttt cgaccgcggg
     2761 tgcgcgcctg ggaaaggagc cgagcgttac tgagtcggcg cgggcgctcc aggaatacag
     2821 agcggggcct ggcagcgatt tccgggattg cagtcggcgt cggtcgcgca ggggaatgcg
     2881 cactttccga actgcgtgtt gcagcgatga agcagacact gagcatccgt cgagcaaggt
     2941 agagccgcgg gcccgggttg cgacatttgt ggcgcggccg gtgttgcggg tgctggtgcg
     3001 ggagccggcg cgacggtagg agccggttga gcaggctgct gtgggtattg ttgctgtggg
     3061 tactgctgct gcggatattg ctgttgctgc tggggatatt gttgctgcgg gtactgctgc
     3121 tgcggataac cgccttgctg cggcggcggc tgatcttcgc tcttgcaacc gatggtgatg
     3181 ctcggaagcg ctgcgacggc aagcacacac acggttcgaa cgcgcaagaa agatcccatc
     3241 atgaaacgct ctccttgttt ccgctgtcg
//
LOCUS       DASTKS010000348         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_725073, whole genome shotgun sequence.
ACCESSION   DASTKS010000348 DASTKS010000000
VERSION     DASTKS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725073"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFQ35_07225"
     CDS             complement(<1..771)
                     /locus_tag="VFQ35_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9930459.1"
                     /translation="MNIAVIGAGLSGLVVARACAARGDSIRVFEAEPRAGGQLHTLRE
                     HGFVVEYGAEGFVAGSAVVPALAADLGISELLQEQRLNLSYGFDGSTLTELGPGEAAR
                     FLGFQVKSEELGRGVRTFAGGMQTLSDALVQRLPANVSLELGRRTTSLRAGGRELVLG
                     FDNGPEIRCDAVVVATSAAHASALLAAEFAAPARALSDSETLSSASVSLAFRQKDVAH
                     PLEASGFVIAVDAQREGLRAGSISSRKFEGRAPAGHVLV"
     gene            complement(822..1652)
                     /locus_tag="VFQ35_07230"
     CDS             complement(822..1652)
                     /locus_tag="VFQ35_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9930460.1"
                     /translation="MVEQLLEGQPKGSPASANPAAVRGEFGELERFRAMRANEEAPEV
                     FDMATLWVELSLGRARMRECFFSEETCYLAVEAATPPTRTDRRLLPRKLRVLERVLLR
                     GGQKSVAAELNLAPSTVAIIAGGCLRAMGIDNGASRAPLPLWMSLHAFHANTIYREGR
                     ISTISHGGRTYSILAVPRPERRLQPLVSPAEYAVLRLLVEGMSHAEMALVRHTSVRTI
                     ANQLASAFHKLGVSGRSELLCQLVADRSPFPQLPPIKAQHSDTQELRHARPIADVVNG
                     "
     gene            complement(1845..2762)
                     /locus_tag="VFQ35_07235"
     CDS             complement(1845..2762)
                     /locus_tag="VFQ35_07235"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9930461.1"
                     /translation="MALLRQQRTERTVLVEPELIIGRSPSCGLQVVEPYVSAQHAALR
                     WGGSRWELKDLGSRNGTFLNGARIEVGKAYAVVEGSELAFGSEAEVWRVEDASPPEAM
                     AIPLDGGEPITPENDVIGLPTSDSPQASIFRDVDGLWKLETVDGDRVVLDSHKPFQVA
                     GRPWRFASPEILGPSSSSLMAASDTPVSLRFNVSRDEEHVELCAVHGDLVVDLGSRQH
                     NYLLLTLARARREDEKSGFPEATCGWVHQEDLLAQLHITSTQLNIDVFRIRQHFARVG
                     LPGVANVIERRPRSKQLRLGFKSFEINVI"
     gene            complement(3011..3499)
                     /locus_tag="VFQ35_07240"
     CDS             complement(3011..3499)
                     /locus_tag="VFQ35_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930462.1"
                     /translation="MTTAKKKTTKPRSKSPTSARVNVAGAKAMVQAAGYSATPLPAKL
                     GIHESERVGLVNAPKGFEALLEPLPKGVRLVRSPRTACDLCIWFPRDRAELGRDMSLR
                     VQQLGPRGIWVCWPKKASGVATDLDENGIRDVALPAGLVDFKVCAIDATYSGLKLQRR
                     KT"
     gene            complement(3504..>3687)
                     /locus_tag="VFQ35_07245"
     CDS             complement(3504..>3687)
                     /locus_tag="VFQ35_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930463.1"
                     /translation="DFVVRSNLFELEWPPGSGKHKQFPEVDRAMFFSLATAREKIIPA
                     QRPFLDRLSERHSAAR"
BASE COUNT          635 a         1203 c         1195 g          654 t
ORIGIN      
        1 caccagaacg tgaccggcgg gggcgcggcc ctcgaacttc ctcgatgaaa tgctcccagc
       61 tcgcagcccc tcccgctggg catcgaccgc gatgacgaag ccgctcgcct cgagagggtg
      121 agccacgtcc ttttgccgga atgcgagcga tacactggcg ctcgagaggg tttccgagtc
      181 gctgagggca cgcgcgggcg ccgcgaactc ggctgccaag agcgcgctcg cgtgcgcggc
      241 gctcgtcgca acgaccacgg cgtcgcagcg aatttccggc ccgttgtcga agccgagcac
      301 gagctcacgc ccgcccgcgc ggagcgaagt cgttcgtcgg ccgagctcga gggaaacatt
      361 ggccggcagg cgttggacga gcgcatcact cagtgtctgc atcccgcccg caaaggtccg
      421 cacgccgcgc ccgagctcct cactcttcac ctgaaagccg agaaagcgcg cggcttcgcc
      481 cggcccgagc tcagtgaggg tgctcccgtc gaagccgtag gagaggttca ggcgctgctc
      541 ttgaaggagc tccgaaatgc cgaggtccgc ggcgagcgcc gggaccacgg cgcttcccgc
      601 gacgaaccct tccgccccgt actcgacgac gaacccgtgt tcgcgcagcg tgtggagctg
      661 gccgccagcg cgcggctcgg cttcgaagac gcgaatcgag tcgccgcgcg ccgcgcacgc
      721 acgggcgact acgagtccgc tgagcccggc gccaatcacc gcaatgttca tggggatggc
      781 ggagctttac accctggcgc ggcgacgcaa agtgctcggt ctcagccgtt tacgacatcg
      841 gcgatcggtc gcgcgtggcg caactcttgc gtatcgctgt gctgtgcctt gattgggggg
      901 agctgcggga acggcgatcg gtccgccacg agctggcaga gcagctcgga gcgccccgag
      961 acgcccagct tgtgaaacgc agaagcgagc tggttggcga tggtccgaac cgaggtgtgt
     1021 cgcacgagcg ccatttcggc gtgcgacatt ccctcgacga gcagacgcaa gaccgcgtac
     1081 tcggccggcg acacgagcgg ttgaagtcgg cgctctggcc gcggcacggc caaaatcgag
     1141 taggtgcgtc cgccgtgcga gatggtgctg atgcgaccct cgcggtagat ggtgttggcg
     1201 tgaaacgcgt gcaggctcat ccacaacggg agcggcgcac gggacgcgcc attgtcgatt
     1261 cccatcgcgc gcaggcaccc acccgcaatg atggcgacgg tcgagggcgc gaggttcagc
     1321 tccgccgcga ccgacttctg cccgccgcgc agcagcactc gctcgagcac gcgcagcttg
     1381 cgcggtagca ggcgtcgatc ggtgcgcgtc ggcggcgtcg ccgcttcgac cgccaagtaa
     1441 caggtctcct cgctgaagaa gcactctcgc atgcgcgctc ggccgagcga gagctcgacc
     1501 cacaacgttg ccatatcgaa aacttcagga gcttcttcgt ttgcgcgcat cgctctaaaa
     1561 cgctcgagct caccgaactc gccacgaacg gcggccggat ttgccgacgc cggggaccct
     1621 ttcggctgtc cctctagcaa ttgctcgacc atgggctctc ctcctggata acggctgaac
     1681 ccccctggga cgcccagatc ccgcgcgacc gagagcgcac cggacctcca cgtcgaaggt
     1741 atttgaccga gcacccccct tcgtgtcgat tacagcgaat tacacgggat tttcacacga
     1801 tttcacgcgt cgacgcattc cgcgtacaac acctgaaatc ggcgtcaaat cacgttgatt
     1861 tcgaagctct tgaagccgag ccggagctgc ttgctgcgcg gccttcgttc gatcacgttg
     1921 gcgacaccgg gtaatccgac gcgcgcgaag tgctggcgga ttcggaacac gtcgatgttc
     1981 aactgcgtgg aggtgatgtg cagctgcgcg agcaggtctt cttgatgcac ccagccgcac
     2041 gtcgcctcgg gaaagccgct cttttcgtcc tcgcgtcggg cgcgcgcgag cgtcaaaagg
     2101 aggtagttgt gctggcgact cccgaggtcc accaccagat cgccgtgcac cgcgcagagc
     2161 tccacgtgct cttcgtcgcg cgagacgttg aagcgcagcg acacgggcgt gtccgacgcc
     2221 gccatgagcg acgacgacga tgggcctagg atttccggcg acgcgaagcg ccagggccgt
     2281 ccggccactt ggaaaggctt gtggctgtcg agcacgacgc gatcgccgtc gaccgtctcg
     2341 agcttccaga ggccgtcgac gtcgcggaag atgctcgcct gagggctatc gctcgtcggc
     2401 agaccaatga cgtcgttttc gggcgtgatg ggctcgccgc cgtcgagcgg aatcgccatt
     2461 gcttctggcg gcgaggcgtc ttccacgcgc cacacttcgg cttcgctacc gaaggcgagc
     2521 tccgagccct cgactacggc gtaggcctta cccacctcga tgcgcgcccc attcagaaag
     2581 gttccgttgc ggctcccgag atctttgagc tcccagcgag agccacccca gcgcagcgcc
     2641 gcgtgctgcg cggacacgta gggttcgacc acttggagcc cacagctcgg cgaacggccg
     2701 atgatgagct cgggttcgac gaggaccgtg cgttctgtgc gttgttggcg gagcagtgcc
     2761 atttcgagcg ggcgtcccga gttggagaga tttagaggac ctcgagcgag cccgggcctt
     2821 cgggatcttc ctatcttgcc gtcgctaggg agttccatgc gatagctttt gggtcaccgg
     2881 ctacgcaagt gggtcaccgg tgcatcagtg cagtaataac caccggtcgg ccggctagct
     2941 agccaatttg tgtcttggaa gtgtctcctc cgcgctgagt gctcgagcct catctttcac
     3001 ggatcattcc tcaggtctta cggcgctgca gcttcagccc ggaataagtc gcgtcgatgg
     3061 cgcagacttt gaaatcgacg agccctgccg gtagggcgac atcgcggatt ccgttctcgt
     3121 ccaagtcggt cgcgacccct gaggctttct tcggccagca cacccagatc ccacgcgggc
     3181 cgagttgctg aactcgcagg gacatgtcgc gcccgagctc cgcgcggtca cggggaaacc
     3241 aaatgcagag gtcgcaggca gtgcgcggcg aacgaacgag gcgaaccccc ttgggtagcg
     3301 gttcgagtag cgcttcgaag cctttgggag cgttgacgag ccccacgcgc tcgctctcgt
     3361 ggattccgag cttcgctggc aatggggtcg cggagtagcc ggccgcttgc accattgcct
     3421 tcgcgcctgc aacgttcacg cgagcgctcg tcgggctctt cgagcgaggc ttcgtggtct
     3481 tcttcttggc ggtggtcatc gaactaccgc gcggccgagt gccgctcgct gagccgatcc
     3541 aagaaaggcc tttgggcggg aattatcttt tcgcgagccg tcgcgagcga gaagaacatc
     3601 gcgcgatcca cttcggggaa ttgcttgtgc ttgccggagc cgggcggcca ttcgagctcg
     3661 aaaaggttgc tcctcacgac gaagtcg
//
LOCUS       DASTKS010000349         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_278543, whole genome shotgun sequence.
ACCESSION   DASTKS010000349 DASTKS010000000
VERSION     DASTKS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278543"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(506..1141)
                     /gene="hisG"
                     /locus_tag="VFQ35_07250"
     CDS             complement(506..1141)
                     /gene="hisG"
                     /locus_tag="VFQ35_07250"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET9930464.1"
                     /translation="MSRPITIAVPKGRVIKALAPFFKRAGVDTTSLEADDRRLIRETA
                     DGSLRFLLLKPDDVPTYVEYGTADLGVSGRDVLLERAYDLYQPLDLGVGRCRMAVAGP
                     RGATIPALPRVATKFPRVASEYFASRGIQAEIIYVGGSVELAPLVGLSDVIVDLVESG
                     ATLRDNNLVEMETICQISSVLVANRSLFKLRHREIHPLVEKLRAAVSEAAR"
     gene            complement(1138..2409)
                     /gene="murA"
                     /locus_tag="VFQ35_07255"
     CDS             complement(1138..2409)
                     /gene="murA"
                     /locus_tag="VFQ35_07255"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9930465.1"
                     /translation="MDAIQIRGGKQLHGTIQVSGAKNAALPILCATLLSDGESLLRNV
                     PNLRDIHTTSELLRFLGRDVVCQPPEVRVQAAKREPRPEAPYDLVRQMRASVLVLGPL
                     VARYGRAKVSLPGGCAIGARPVDQHLMGLEALGAKIQVEHGYIHAEAKRLRGAEVVFD
                     MQTVTGTENLMMAAALAKGRTTLVNCACEPEVEELGRVLNKMGARVEGAGTSIIHIEG
                     RDELEPFDHAIIADRIEAGTFMAAVGAAGGDVLLQHAPLDDLEPVVVKLRKAGLEIER
                     EGDGVRVRRLGKLSGVDVSTAPHPGFPTDMQAQFMAMASISSGRSVMTENVFENRFMH
                     VPELLRMGARIETHSNTAIVEGVPLLSGANVMATDLRASASLVIAGLVAEGETVVHRV
                     YHLDRGYEAIEKKLAGAGANVTRIRVESEAP"
     gene            2603..>4192
                     /locus_tag="VFQ35_07260"
     CDS             2603..>4192
                     /locus_tag="VFQ35_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9930466.1"
                     /translation="MVRGPFVRLSRAALAAGMLVSLQSKASPTPSLDLRGFQASTDPK
                     ASLYLETATTPGHLQWNAGAWASYAHRLVELEDASGHVVSVPVRHQLSLDYVASLGLT
                     DRLALGFVLPTVAYQSGSENVGQYVPGASTLPKAAIGDLAFTAKALLLPRGDLGGFAL
                     AALARVSVPTGSDTSYVSESAAVGELRVLGELSLVALTLRATTGMRVRGAEQTYAGAD
                     FGHDLPWGVGLSFMPQVFGIDPGGHFQGTLEAHGAIALTPKFASKPQSPALLGASVRY
                     GIGDVFLTLGAELPLSDAIGAPRVRGVLGFMYAPRVIDTDQDGVPDEKDECVELAEDR
                     DHFEDGDGCPDFDNDDDGVPDDQDKCPALKEDQDGFQDEDGCPDLDNDQDGLPDAQDK
                     CPLEPGARDAKEPGCPQKDRDLDGIPDRSDRCPAGAEDRDTFQDDDGCPDPDNDQDGS
                     PDAEDDCPKAPGAERSEAKLNGCPSPDKDGDTYDDARDACADQPEDFDGEADTDGCPE
                     PAGGAALARIEQQGNGLTLVLA"
BASE COUNT          749 a         1392 c         1349 g          702 t
ORIGIN      
        1 ggaggctccg gttttgccgg cggagtcgct tgcgcggagc tcgcggcggg ctctggagct
       61 gcggcggacg cggctggcgc cggggcaggc tcgggttgcg cagccggttg tgcggagcct
      121 ccacaaccca tgagtccaat caatgtgacc accccaacca gtgtcgatct cataggctgc
      181 tccttctgca tctgcattat gcaaatgcat tgagcaatcg acgtgccacg cacccgtcgc
      241 cacttatgac actggaatta gtagctttcc ccggacaagc agagagaagc aaacactcga
      301 attgttcagt gaatgggttc tctcagcaat gcgcgcaccg cagaatccat gctgcgcgac
      361 tcactcactg cagcgagcaa gtcgcaaccc cgttttgcac cgactctggg gcgtggcgta
      421 atcgaggctt cttgaattcg cgaccacggc ggagaaaatc ctgcgccgcg aactcgcgta
      481 acgcaacgac tgcacgtgag tcgaatcagc gcgcagcttc ggatacggcg gcacgcaact
      541 tttcgaccaa ggggtgaatt tcgcggtgcc ggagtttgaa cagagatctg tttgcgacga
      601 gcacactcga gatctggcaa atcgtttcca tctcgacgag attattgtcg cgaagcgtgg
      661 cgccagattc gacgaggtcc acgatcacgt ccgagagccc tacgagcggc gcgagctcga
      721 ccgagcctcc gacgtagatg atttcagcct gaatgccacg gctcgcgaag tactccgaag
      781 cgactcgtgg gaacttggtc gcaacgcgag ggagcgcggg aatggtagcg ccgcgcggtc
      841 ccgcgacggc catgcggcaa cgaccaacgc cgaggtcgag gggttgatag aggtcgtagg
      901 cgcgctcgag caacacgtcg cgaccgctga cccccaaatc ggccgtgccg tactccacat
      961 aggtcgggac gtcgtcgggc ttcagcaaca gaaagcgcag cgaaccgtcc gccgtctcgc
     1021 ggatcaagcg gcggtcgtcg gcctccagcg aggtcgtatc gacgccagcg cgcttgaaaa
     1081 agggcgccag cgccttgatg acgcggccct tcggtacggc gatggtgatg ggacggctca
     1141 tggggcctcc gattcgacac gaatacgcgt gacgttcgcg ccggcgccgg cgagcttctt
     1201 ttcgatggcc tcgtaaccgc gatccaagtg atagacgcgg tgcacgacgg tctcgccctc
     1261 ggcgacgagt ccggcgatga ccagcgacgc gcttgcgcgg aggtcggtcg ccatcacgtt
     1321 cgcgccggag aggagcggca ccccttcgac gattgccgtg ttgctgtggg tctcgatacg
     1381 agcccccatt cgcaagagct ctgggacgtg catgaagcga ttctcgaaca cgttttcggt
     1441 catgacgctg cgaccgctgc tgatgctcgc catggccatg aactgcgctt gcatatcggt
     1501 cggaaagccc gggtgaggcg ccgtactcac gtcgactcca ctgagctttc cgaggcgccg
     1561 cacgcgcacg ccatcgcctt cgcgctcgat ttcgagccca gctttgcgca acttcacgac
     1621 gaccggctcc agatcgtcga gcggcgcgtg ctgtagcagc acgtctccac ccgccgcgcc
     1681 gaccgccgcc atgaacgtcc ccgcctcgat gcgatcggcg atgatcgcgt ggtcgaacgg
     1741 ctcgagttcg tcacgaccct cgatatggat gattgaagta ccggcgccct cgacacgagc
     1801 gcccatcttg ttgagcacgc ggccgagctc ttcgacttcg ggctcgcaag cacaattgac
     1861 gagcgtggtt cggcctttgg cgagcgccgc ggccatcatc aggttctcgg taccggtcac
     1921 ggtctgcatg tcgaacacga cctcggcacc gcgcagccgt ttggcctccg cgtggatgta
     1981 gccgtgctcg acctgaatct tcgctcctag ggcctcgagc cccatgaggt gctgatcgac
     2041 cgggcgcgcg ccgatcgcgc agccaccggg cagtgacact tttgctcgcc cgtaccgcgc
     2101 gacgagcggc ccgagtacga gcacgctcgc gcgcatctgg cgcaccaggt cgtacggagc
     2161 ttccggccgc ggctcgcgct tcgctgcctg cacccggacc tcgggtggct gacacacgac
     2221 gtcccggccg agaaagcgca aaagctcgct cgtcgtgtgg atgtcgcgca agttgggaac
     2281 attgcgcaac aagctctcac cgtccgaaag cagcgtcgcg cagaggatcg gcagagccgc
     2341 attcttcgcc ccgctcactt gaatggtgcc gtgaagctgc ttgccgccgc ggatctgaat
     2401 agcgtccatc gctaggggcg ggccgtacgc tgtcccggcc acaagcgcaa gcattcgacc
     2461 gtgcgggatc cggaaatcgc gagcgagact gggcaagtgc ccccgaacca gctcggcctt
     2521 tcggcctgtc gccgcgcgag gctctgagcg gttcgcttgc gggcgtgccc aacggggcca
     2581 tccgatagta ggctcagccg ccatggttcg gggcccgttc gttcgcctat cccgcgctgc
     2641 tttggccgcg ggcatgctcg tttctctgca gtcgaaagcc tcaccgactc cgtctctcga
     2701 cttgcgcggc tttcaggcgt caacggatcc caaagcgtcg ctctacctcg aaaccgccac
     2761 gactccgggt cacttgcagt ggaacgcggg cgcctgggcc tcttacgccc accggctcgt
     2821 cgagctcgag gatgcgagcg ggcacgtcgt cagcgtcccc gtgcggcacc agctttcgct
     2881 cgactacgtg gcttcgctcg gcctcaccga tcgcctcgcg ctcgggttcg tgctgccgac
     2941 ggtcgcgtat caaagcgggt ccgagaatgt cgggcagtac gttcccggag cttcgacgct
     3001 gccgaaagcc gcgattggcg atctcgcctt cacggccaaa gcgctgctct tgccgcgcgg
     3061 agacttgggc ggcttcgcgc tcgcggcctt ggcgcgcgtc tcggtgccca ccgggagcga
     3121 cacctcctac gtttcggagt ccgcggccgt cggcgagctg cgcgtgctcg gtgaactcag
     3181 cttggtggcg ttgacgcttc gagcgacgac cggcatgcgc gtgcgcggag ccgaacagac
     3241 ctatgccggg gccgatttcg ggcacgacct gccatggggc gtcggcctga gcttcatgcc
     3301 ccaagtgttc ggaatcgatc cgggaggcca tttccagggc acgctcgagg cgcacggcgc
     3361 gatcgcgctc acgccgaagt tcgcctccaa gccgcagtcg ccggcgctgc tcggcgcatc
     3421 ggtgcgttac gggatcggcg acgtctttct cacgctgggt gcagagctcc cattgtccga
     3481 cgcgatcgga gcgccgcggg tgcgcggcgt gctcgggttc atgtacgcgc cccgcgtgat
     3541 cgacacggat caggacggag tgccggacga aaaagacgag tgcgtcgagc tcgccgaaga
     3601 tcgcgaccac ttcgaagacg gcgacggctg ccccgacttc gacaacgacg acgacggtgt
     3661 gccggacgat caagacaagt gcccggcgct caaggaagat caagacggct tccaggacga
     3721 ggacggctgc cccgacctcg acaacgatca ggacggcttg cccgacgcgc aggacaagtg
     3781 cccgctcgag ccgggggcgc gcgacgccaa agagcccggt tgcccgcaga aagaccggga
     3841 tctcgacggc attcccgacc gaagcgatcg ctgcccggcc ggagcagaag accgcgacac
     3901 gttccaagac gacgacggct gcccggaccc ggacaacgac caagatggct cgccggacgc
     3961 ggaagacgac tgccccaaag cgcccggcgc cgagcgttcg gaggccaagc taaacggctg
     4021 cccgagccca gacaaagacg gcgacacgta cgacgacgcg cgcgacgcat gcgctgacca
     4081 acccgaagac ttcgacggcg aagccgatac ggatgggtgc cctgagccag ccggcggcgc
     4141 cgcgctcgcc cgcatcgagc aacaaggcaa tggtctcaca ctggtgctcg cg
//
LOCUS       DASTKS010000350         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1172040, whole genome shotgun sequence.
ACCESSION   DASTKS010000350 DASTKS010000000
VERSION     DASTKS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172040"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..3705
                     /locus_tag="VFQ35_07265"
     CDS             103..3705
                     /locus_tag="VFQ35_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930467.1"
                     /translation="MADTTIELEAALDAHAEKSETGKLLQSSWRFDKLLLTDSLQAVG
                     VLFPHYSRHDASHSRRIASNIAQILGERVKQLGPTDLWLLLEAAHWHDVGMIVDDDSA
                     REWWQQPDFREHLERLREGKDTRLQQAARDVLGGSDEALRKTYGKHWQLELRRSIAFV
                     LADYARGRHASRSSEIVLEPSRASIGSPRGLIPTRLFRWLAQIALVHGCDAATAMETL
                     AHRENGVGTDVCHPRFAGFMLRLGDLLDLDNGRFCASLARTIGELPPSSVQHVGKHAA
                     ITRFLLSPEKIEVHAVCNDYHDPSLHHHGISAYDVYEATESWLGWLRDELAFLNQWWS
                     DIAPPGFSGAPGYGAISARLDGYELRDGHSPRFGVDADTLFQLVAGKNLYAQPTSTWL
                     RELLANAKDATLCRAWHEQVQEHSSEELASAAWRAKSDPIAEMMRLLGSRFSIEVSLS
                     PVPEPDCGGEDAARASSRASGQRAKSSAHFSKHAKPTSRWRFSIRDQGVGIGPKDLEY
                     LLTVGASSKRRRRFPQPTSMPPYWEPTGSFGIGLQSVFQVTDRIVIRTHHFESHECRE
                     IVLNNPAPFPPGVSSTVPRAWGAAYIRRLKEHEVAIHRLYSPGTVVEFDIDLNHLPPF
                     ETEHFQGSGEKPFSDVRDRAFFDFVSVPQPALWPALWWSLLCKWRVGEEYLPKLVSTA
                     FKAPTDSASNPPRAETEVFFHGETAIRFRFGLIDGEATRTDGGSGSGMPLGSQPRRFA
                     YQGVDFLAFPRTESLRERIRELLPNFPWEKIGIEIEWFEGPAWKYLNIDRDRLTPEGE
                     RRLCEALKRALNGVMPKYLEALERHRPADESTQLISLYVQLYCDGVEPSQLLKNVAWR
                     WLKFDWLLVDGLEQQPLGSIVPVPAVYTTSSPVGDGAAPQAPNKPFHLSIDRHKELDP
                     ERMPGVGQSALSCALQLQLFPKNPDARELRVKRAFGTKEQWKANKERFRRQFDWFDDF
                     ILKHFAAKKLGTTKSRAPEGDGEDFVTTQYDLTPRPVMQWSQPPAADEVEEDAGFWQE
                     LALIFGRTPRVRAALPSHPDYRGLEVGHAICKEKAWIAEGSHILRPSFVCPFELYGLS
                     IELEHVEEYVQWVAQVRRERDEALRVLSQSEVETEVARVTWQFVCDVDKLMTDATTHN
                     LFAEIKRAYNTQDVKRRLENFFSLRLDDEPQPAGRKERKEALRKEKERRSRAST"
     gene            3787..>4288
                     /locus_tag="VFQ35_07270"
     CDS             3787..>4288
                     /locus_tag="VFQ35_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HET9930468.1"
                     /translation="MSSLARIVRVSAAGLVAFLVLTTLSSRARAWGPIGHETVAHLAE
                     RNLTSKARARVAELLGPRASLASIATWADDVRKARPETKNWHFIDIENDARTRRPDVA
                     RFCPEHDCVVDQIDMARQRLVDEKLTLGERSEALKFLVHFVGDLHQPLHCADDDDRGG
                     NEKLLLV"
BASE COUNT          802 a         1327 c         1362 g          797 t
ORIGIN      
        1 ttcacgcccg cacgtcaccc cgatagcaaa cagtttgggt gcgtagcggc ccggcacgca
       61 gtcgcgcggt gcaggccaac tgctccgggt gacgacgaca acatggcaga tacaaccatt
      121 gagctcgaag cggcgctgga cgcgcacgcc gagaaaagcg aaaccgggaa gttgctgcag
      181 tcgagttggc gcttcgacaa acttctcttg accgacagct tacaagcggt cggtgtgctg
      241 ttcccgcact acagtcgcca cgacgccagt cattcgcgac gaattgcttc gaacatcgct
      301 cagatcttgg gcgagcgcgt caagcagctc ggcccgaccg atctctggct cttgctcgaa
      361 gcggctcact ggcacgacgt cggcatgatc gtggacgacg actcggcgcg cgaatggtgg
      421 cagcagccgg attttcgcga gcacctcgag aggctacggg aaggcaagga cacgcggttg
      481 cagcaagcgg cccgcgacgt gctcggcggg agcgacgagg cattgcgcaa gacgtacggt
      541 aagcattggc agctcgagct ccgtcgatca atcgcgttcg ttctcgcgga ctacgcgcgc
      601 ggtcgacacg cgtcgcgctc gagcgagatc gttctcgagc catcgcgcgc aagcattggc
      661 tcccctcgcg gtttaatccc gacgcggctc ttccgctggc ttgcccagat tgcgctggtt
      721 cacggctgtg acgccgcgac cgcgatggag acgctcgcgc acagggagaa tggtgtaggc
      781 accgacgtgt gtcatccgcg cttcgccggc ttcatgctgc ggttgggtga cctcttggac
      841 ctcgacaacg gccgcttctg cgcgagcctc gcgcgcacca tcggcgaatt gccgccgtcg
      901 tccgtgcagc acgtcggcaa gcacgccgcg atcactcggt tcttgctttc gcccgagaag
      961 atcgaagtgc acgccgtttg caacgactac catgatccgt ctctacatca ccatggcatc
     1021 agcgcttacg acgtctacga agcaacggag agttggcttg gttggctgcg cgacgagctt
     1081 gctttcttga accaatggtg gagcgacatc gctcccccgg gtttcagcgg ggcacccggt
     1141 tacggagcca tttcggcacg gctcgacgga tacgagttac gcgatgggca ctcgccgcgc
     1201 tttggcgtcg acgcggacac gttgttccag ctcgtggcgg gcaagaactt gtacgcgcaa
     1261 cctacgtcga cctggctgcg cgagctcctg gcgaacgcga aggatgcgac cctctgccgc
     1321 gcgtggcacg agcaagtcca agaacactcg agtgaggagc tcgcgagcgc ggcctggcga
     1381 gcgaagtcgg accctatcgc ggagatgatg aggctgttgg ggagtagatt cagcatcgag
     1441 gtctctctct ctcccgtccc ggagcccgat tgtggcggtg aggacgccgc acgagcttcg
     1501 tcccgagcga gcggccagcg tgcaaaaagc tccgcgcact tcagcaagca tgcgaagccg
     1561 acctcgcgct ggcgcttctc cattcgcgac caaggcgtcg gtatcggacc gaaagacctg
     1621 gagtatctgc tcacggtggg cgcctcttcc aagcgcaggc gccgctttcc acagccgacc
     1681 tcgatgcctc cctattggga accgaccggc agctttggga ttgggctgca aagcgtcttt
     1741 caggtgaccg accgcatcgt gattcgtact catcacttcg agagccacga gtgccgcgag
     1801 atcgtcctga acaacccggc gccttttcct ccgggcgtgt catcgacggt ccctcgcgcg
     1861 tggggcgcgg cgtacattcg cagactcaaa gagcacgagg tcgcgattca tcgcctctac
     1921 tctccaggca ccgtagtcga attcgacatc gacctgaacc acttgccgcc tttcgagacg
     1981 gaacactttc aaggttcggg tgagaagccg ttctccgacg tgcgcgaccg cgcgttcttc
     2041 gatttcgtca gtgtgccgca gcccgcgttg tggcccgcgc tttggtggag cctgctttgc
     2101 aagtggcggg tcggcgagga gtatctgccc aagctggtgt ccactgcgtt caaggcacca
     2161 acggattcgg catcgaaccc gccgcgcgcc gaaacggagg tgttttttca cggcgaaacg
     2221 gcgatccgct ttcgcttcgg gctgattgac ggcgaggcga cgagaaccga cggtggcagc
     2281 gggagcggca tgccgctggg aagtcagccg cgtcgcttcg cgtatcaagg cgtcgatttt
     2341 ctcgcgtttc cccgcacgga gtcgttgcgc gagcgcattc gcgagctcct cccgaacttc
     2401 ccctgggaaa agatcgggat cgaaatcgag tggttcgagg gtcccgcttg gaagtacctc
     2461 aacatcgacc gcgaccggct gacgccggaa ggcgaacggc gtctctgcga ggctctgaag
     2521 cgcgccctaa acggcgtgat gccgaagtac ctggaggccc ttgagcgtca ccgtccagcc
     2581 gacgaatcga cgcagctcat cagcttgtac gttcaactct attgcgatgg cgtggaaccg
     2641 tcacagttgc tgaagaatgt cgcgtggagg tggttgaagt tcgattggct cctagttgat
     2701 ggcctcgagc agcagcccct tgggtcaatc gttcctgtcc ccgccgtcta caccacgtct
     2761 tcgccagttg gagatggcgc cgcgccccaa gcgccgaaca agccgtttca tctcagtatc
     2821 gatcggcaca aggagctcga ccccgagcgc atgcccggcg ttggccagag cgcgctcagc
     2881 tgtgccttgc aactccagct gtttccgaag aaccccgacg cgcgtgagct ccgcgtcaaa
     2941 cgcgccttcg gaacgaaaga gcaatggaag gctaacaagg agcggttccg gcgccagttc
     3001 gattggttcg acgacttcat tctcaagcac ttcgccgcca aaaagttggg cacgaccaag
     3061 tcccgtgctc cggaggggga cggagaggat ttcgtgacga cccagtacga tcttacgccg
     3121 aggccagtca tgcagtggtc acaacccccg gccgccgacg aagtcgagga ggacgccggt
     3181 ttttggcagg aattggcgtt gattttcggg cgaactcctc gcgtccgtgc ggcgctgccc
     3241 agtcacccgg actaccgtgg cctcgaggtt ggacacgcga tatgtaaaga gaaggcgtgg
     3301 atcgcggagg gaagccacat cctgaggccg tcgttcgtgt gcccgttcga gctctacgga
     3361 ctgagcatcg agctcgagca cgtcgaggag tacgtgcagt gggtggcgca ggttcgccgc
     3421 gagcgagacg aagcgcttcg ggtgctgtct cagtcggaag tcgagactga ggtcgcgcgt
     3481 gtgacctggc agttcgtctg cgatgtagac aagctcatga cggatgcgac cactcacaac
     3541 ctatttgccg aaatcaagcg agcgtacaac acgcaggacg tgaagcgccg tctcgaaaac
     3601 ttcttcagcc tgcgcttgga tgacgagccc cagccggcgg gtcgcaaaga gaggaaggaa
     3661 gcgctccgta aggagaagga gcgacgctct cgggcttcaa cgtaacgctc gcgcgaggct
     3721 cctacggcga gccgcgcgcc gacgttgctc ggcggagagg cctgcgcagt gctagacaac
     3781 gcgaagatgt catctcttgc gcggatcgtt cgtgtgagcg ctgccggcct cgttgctttc
     3841 ctcgtgctca ccaccctctc gagtcgcgct cgcgcttggg gacccatcgg tcacgaaacg
     3901 gtcgcccacc tcgcggagcg gaacctgact tcgaaggctc gggcgcgcgt ggcggagctg
     3961 ctcgggccgc gcgcttcgct ggcgagcatc gcgacgtggg cagacgacgt tcgcaaagcg
     4021 cgtcccgaga ccaagaattg gcacttcatc gatatcgaga atgacgcgcg cacgcgccgc
     4081 cccgatgtcg cgcggttttg tcctgagcac gactgcgtgg tcgatcagat cgacatggct
     4141 cggcagcggc tcgtcgacga aaagctgacg ctcggcgagc gtagcgaagc gctcaagttt
     4201 ctggtgcact tcgtcgggga cttgcaccag ccgttgcact gcgcagacga cgacgatcgc
     4261 ggcggaaacg aaaagctctt gttggttc
//
LOCUS       DASTKS010000351         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_279084, whole genome shotgun sequence.
ACCESSION   DASTKS010000351 DASTKS010000000
VERSION     DASTKS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279084"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            807..>2028
                     /locus_tag="VFQ35_07275"
     CDS             807..>2028
                     /locus_tag="VFQ35_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930469.1"
                     /translation="MRDEAPSSAFDVLFHDPSTLSRAELEGHAQALWAVLRIADVVHR
                     ARDLDELVEAAVEAITRYTNFPSVALFHVNEQDGHLHLAAARGFAEDVVEKARRLPIV
                     GSLTGLAVTRRALVTSADLRDDPRVEPSVRAALQHDGFVGVASIPLFDRDRVIAAMNL
                     IYRQRSHLSDSERRILMAIGQTIGLAMSLRLAAAEQQKLEEQARRAQQVESLGVLAGG
                     IAHDFNNILTGILGNVSLARALIDSSQQVELDELMMEAEQACDRAVGLVRQLLTFARG
                     GAPLRRPTRDLSTLVEEAARFAIRGTSIELRFECEPELGTVEIDPGQIMQVVQNLVLN
                     AVQASRSGGTVRVRLSRIEAESGAQRTRVRLVVEDDGIGIAPEALPRIFEPYFTTRAG
                     GNGLGLATTHSIVRR"
BASE COUNT          358 a          626 c          668 g          376 t
ORIGIN      
        1 ggaggctcag cgaatctctg tttgggaatg cgcgttccgg tcgcgctaaa aaaagccacg
       61 attcactctg aaggggtgag cagaagaggg gggcgaattt gcgtaaaaac tcggggcgcc
      121 agcgtcacat ttccagcgcc cgcacgtcgt cgcgatggag cagcacgctc ggtgcggtgg
      181 gcctcagtgc gccaccgccg ctggcgggag aagctcggtt tagacctttt ttggggcccg
      241 ctcttcgaga cggcgacttc ggagggcccg cgctgggcat gcgtgagggt attggtaact
      301 cgcagagaac cacccgctga gatcagaatt cacgaagagc gaaaagcaaa attcagtgtg
      361 cacgaaactt gccccaaacg agccgattcg gtcatggctg gctttttcga agtagttctc
      421 atgaccagct cgacttcctc gtgccgtctc tgccgaaatc tcactgacga accggatacg
      481 ctttgcgctt cgtgcaagcg cgcgcggggg caccgggtcg cctcgacgct cgcgcgggcg
      541 ctggcggact cgagctatca aagcgcgtgc ctggcctcgc tgcctcgcga agcccgagat
      601 cgcttcgcac aagcgctctc ccgctcttat ctgctgcgcg aagcgcccgt accgcacgaa
      661 gcctcctcga aacccacgtc ggcgccccgt ttggcgaaat gtcatccagc ggccgctttg
      721 aacgggctcc gtcgcgcggg ctgagccaac acgaaccttg attggcgctc gatttcgcgg
      781 gcgctagttt gaaagagccg gtgcgtatgc gagacgaggc tccgagttct gcgttcgacg
      841 tgctgtttca cgacccgtcg acgttgagtc gggcggagct cgaagggcac gcacaggcgc
      901 tttgggcggt tctgcggatc gccgacgtcg ttcaccgggc tcgcgatctc gacgagctcg
      961 tcgaagccgc cgtcgaagcg atcacgcgat atacgaactt cccctcggtc gcgctctttc
     1021 acgtaaacga acaggacggc catctgcacc tcgccgccgc gcgcggcttc gccgaggacg
     1081 tcgtggagaa ggcgcgccgc ctacccatcg tcggcagcct cacgggtctc gccgtcaccc
     1141 gtcgcgcgtt ggtgaccagc gccgacttgc gagacgaccc tcgcgtcgag ccgagcgtgc
     1201 gcgcggcgtt gcagcacgat ggattcgtcg gggttgcttc gattccactg ttcgatcgcg
     1261 atcgcgtgat cgcggccatg aacctgatct atcggcagcg ctcgcatctc tccgacagtg
     1321 agcgtcgcat cttgatggcg ataggccaga ccatcggcct tgccatgtcg ttgcgtttgg
     1381 cggcggcaga gcagcaaaag ctcgaagagc aagcgcggcg cgcgcaacag gtggagagcc
     1441 tgggcgtgct cgcgggcggc atcgcgcacg acttcaacaa catattgacg ggaattctag
     1501 gtaacgtttc gctcgcgcgc gcactcatcg actcgagtca acaagtcgag ttggacgagt
     1561 tgatgatgga ggcggagcag gcctgcgatc gagccgtcgg tttggtgcgt cagctgctca
     1621 cgttcgcgcg cggcggcgcg ccattgaggc gaccgacgcg cgacctttcc accctggtcg
     1681 aggaagcggc tcgattcgcg attcgcggca ccagcatcga gctccggttc gagtgcgagc
     1741 cggagctcgg gacggtggag atcgatccag gtcagatcat gcaggtcgtg cagaacctcg
     1801 tgctgaacgc cgtgcaagcg agtcgctccg gcggaacggt gcgcgttcgg ctctcccgca
     1861 tcgaggccga gagtggagcg cagcgaacgc gggttcgttt ggtggtggag gatgacggta
     1921 tcggcattgc gcccgaagcg ttaccgcgga tcttcgagcc gtacttcacg acgcgcgcgg
     1981 gcggaaacgg gctcgggctc gcgaccactc attccatcgt gcgacgtc
//
LOCUS       DASTKS010000352         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1172759, whole genome shotgun sequence.
ACCESSION   DASTKS010000352 DASTKS010000000
VERSION     DASTKS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172759"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFQ35_07280"
     CDS             complement(<1..957)
                     /locus_tag="VFQ35_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930470.1"
                     /translation="MMNSQFPARGSKLALSALVLALAGVSPNARAEEDPSAAETAAAR
                     ALAVDGLKLAKAGQCREAVEKLERAEKLRHSPIVLARLGECYVNIGKLVQGTEALRKF
                     LREPLPPDANANLKEAYDHAQVVIQETKGNIAGLTIAVSGGEKGKYTVTIDGVEVAST
                     LVGVEMPVDPGEHVIEVSGPQYLKTSARAKLAPGEKQSISIEAKPDPDAAKQKVEEPV
                     GAAPNVAAPSENRATPQPFSATTTESPPSSGNSKALAYVSYGVGALGIGAGILFGRSA
                     MQDKDQLDAQCPGKVCPPDQEDRLNSAKTKGVVSTIGFGVGAA"
     gene            1229..>2229
                     /locus_tag="VFQ35_07285"
     CDS             1229..>2229
                     /locus_tag="VFQ35_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9930471.1"
                     /translation="MRTRLTPTMLGLFCSLAAAACNSVTGLNDFSVGSGPTQQAQCTT
                     NAECADQSAALGAGGDSSGTTICVKASGACAKVQSVDCDTITGDVSDDRAIIIGSLFA
                     TKGQQATLNLQRQQSAQLAVEEINAVGGVPTPAGNPRKLVMVSCDVGTNLVRASEHLV
                     KDLKIAAIIGPNTSQDTLDVSNKVSVNGGSVIISPSAVASSITGLVDNDLTWLMVPTD
                     TQRAPLMLDQINELEMSLKAARSVSTIKLSVIFQDDALGLGTRQGLDQLVINGKPLID
                     PINFGKNVQISGYDVKVKDQSSLVAKHVAFAPDIVVLAGTAEAIPNIFAPIEQQWTSP
                     "
BASE COUNT          419 a          713 c          684 g          413 t
ORIGIN      
        1 ggccgcaccc actccgaaac caatcgtcga gaccacgccc ttggtcttcg ctgaattcag
       61 ccggtcttct tgatcggggg gacacacttt gccggggcac tgcgcgtcga gttggtcctt
      121 gtcctgcatg gccgagcgac cgaacaagat gcccgcgccg atgcccagcg cgccgacgcc
      181 gtaagagacg tacgcgagcg ccttgctgtt accggagctc ggcggagact cggtcgtggt
      241 cgcgctgaag ggttgagggg tcgcgcggtt ttcgctcggc gccgcaacgt tcggcgctgc
      301 gcccacgggc tcctcgacct tctgcttggc ggcgtcggga tctggctttg cttcgatcga
      361 gatcgattgc ttctcgcccg gagcgagctt cgcccgcgcc gacgtcttca agtactgcgg
      421 gccgctcact tcgatgacgt gctcgcccgg gtcgaccggc atctcgacgc caacgagcgt
      481 cgacgccacc tcgacgccgt cgatcgtgac cgtgtacttg cccttctcgc cgccgctgac
      541 ggcgatcgtc aaaccagcga tatttccctt ggtttcttgg atgacgacct gcgcgtgatc
      601 gtaagcctct ttcaggttcg cgttcgcgtc gggagggagc ggctcacgca gaaacttgcg
      661 cagtgcctcc gtgccttgca ctagcttgcc gatgttgacg taacactcgc cgaggcgcgc
      721 gagcacgatc ggcgagtgcc gaagcttctc ggctctctcg agcttctcga ctgcttcccg
      781 gcactggcct gctttcgcga gcttcaaacc gtcgaccgcc aacgcgcggg ccgcggccgt
      841 ttcggccgct gaaggatctt cttcggcgcg cgcgttcgga gagacgcccg ccaaagcgag
      901 cacgagcgcc gagagcgcga gcttcgaacc cctggcggga aattgactgt tcatcattcg
      961 ttagcccttc acctgcacca cgaagtggac gcttgagcgg gtactacggt ccaaggtgcg
     1021 aattctgaag accaagctca ccgactcgaa cgactcgata tcgagagagc tcgaggctac
     1081 acgtcacagg aatccagtga aagccccgcg cgtgatgacg ggaatcgcca cgcgcaaacg
     1141 tgcaccgctg tctgataaac aaggaactcg atttcctgtc atcggctctc ggccgctcaa
     1201 ggatcggact cggaactgaa ggagcgctat gcgaacgcga cttactccca cgatgctcgg
     1261 cttgttttgc tcgttggcgg ctgcggcatg caactccgtg acgggcctga acgatttctc
     1321 ggtgggctct ggtcccactc agcaagctca atgcacgacg aatgcagaat gcgcggacca
     1381 aagcgccgcg ctcggtgccg gtggagatag ctccgggacg acgatctgcg tgaaggcgag
     1441 cggcgcctgc gcgaaggtgc agagcgtcga ctgtgacaca atcaccggtg atgtgagcga
     1501 cgatcgcgcg atcatcatcg gcagcctttt cgcgacgaag ggacagcagg cgacgttgaa
     1561 cctccagcgg caacagagtg ctcagctcgc ggtggaggag atcaacgctg taggcggcgt
     1621 tcctactcct gccggcaacc cgcgcaagct ggtcatggtg tcttgcgacg tgggtacgaa
     1681 cctcgtgcgg gcgagcgagc acctcgtgaa ggatttgaag atcgccgcga tcatcgggcc
     1741 caacacgagc caagacacgc tcgacgtatc gaacaaggtc tcggtgaacg gtggaagcgt
     1801 catcatctct ccgtcggccg tcgcgtcgag catcaccggc ctcgtcgaca acgatctcac
     1861 ctggctgatg gttccgaccg acacccagcg cgcaccgctg atgctcgatc agatcaacga
     1921 gctcgagatg tcgctgaaag cagcgaggtc cgtcagcacc atcaagctga gcgtgatttt
     1981 ccaggacgat gcgctgggcc tcggcacgcg ccaggggctc gatcagctcg tgatcaacgg
     2041 caagccgctc atcgacccga tcaacttcgg caaaaacgta cagatcagcg gctacgacgt
     2101 gaaggtcaaa gaccagagct cgctcgttgc gaagcacgtc gcgttcgcgc cggacatcgt
     2161 cgtgctcgcc ggcacggccg aagccattcc gaacatcttc gcgcccatcg agcagcagtg
     2221 gacgtcgcc
//
LOCUS       DASTKS010000353         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1619652, whole genome shotgun sequence.
ACCESSION   DASTKS010000353 DASTKS010000000
VERSION     DASTKS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619652"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFQ35_07290"
     CDS             <1..409
                     /locus_tag="VFQ35_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9930472.1"
                     /translation="AAKRTRRADAEERAAEALRQVGLLRWAGRYPGELSGGQQQRVAI
                     ARALVGSPRLILADEPTGNLDSRTSLEVMALFQELGRSGITIVLVTHEPDIATYASRV
                     IVLKDGLVEADRTQVPADARAEVERLAPVPVSA"
     gene            406..1635
                     /locus_tag="VFQ35_07295"
     CDS             406..1635
                     /locus_tag="VFQ35_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9930473.1"
                     /translation="MSLLQTFRIAFRALLRNKMRSFLTTLGVVIGVGAVIAMVAIGEG
                     AKSSVEQAFAAMGTNLLIVMPGANTSGGVRGGFGSQPTLTWDDLKAIQTELPTVKAAA
                     AFLRSTSSVVSEDQNWTTQIGGTSPEYLEIRSWPIAQGRAFTQSDVESSTKVALLGRT
                     VADKLYGPSSNPVGQLVRINNVPFEVIGLLATKGQSAIGQDYDDTVLVPYSTFRSKIQ
                     GGLKNYLSGTIFVQGTSQAELQRTQDAVTSLLRDRHRLAQGVDDDFSIRNLTEMASAQ
                     QQGTETMTTLLASIAFVSLLVGGIGIMNIMLVSVTERTREIGLRMAIGAKPHQILAQF
                     LVEAFTLSSMGGALGVMLGVGIALYLASRFGWPLLIRPDIIAVSVLFSALVGVCFGLY
                     PAYKASRLDPIDALRYE"
     gene            1632..3092
                     /locus_tag="VFQ35_07300"
     CDS             1632..3092
                     /locus_tag="VFQ35_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9930474.1"
                     /translation="MKRSLFARSLGFCAAVSMFFSFGAARAQEKANPSPVTGPTPAPA
                     PNAPTAPNAAPVDTGKLSTSPATTTTELPAAGPVLPLAQALRNGLERHPTLRQAEANS
                     RAADARVDQAFAPLLPQVTATATYSRSTANPVPRAGQVNLPPRDPSFDTYGFWNFGIN
                     ASYVVYDFGQTRGRLRSAEANAEVTKLSERAQRLNIALNVRNAYFQARQQKALLLVAT
                     ETLTNQERHLGQIRGFVEIGTRPRIDVVQAQADRESAELQRVNAENNYAIAKAQLVQA
                     MGLDNAPPFEVSDETLAPLDLEEAEPNRLYSRALATRPDVLAAVGSVRAQELSESALK
                     GGYGPSLSVSTGVTEAGAALDTLRWNWNAQAALSWPIFQGGITKAQVREARANVDAAT
                     AQVNLVKQQVLLAVEQARIAVVGAKRALATADVLVQNARERLNLAEGRYQTGVGSIIE
                     LGDAQLALTNAQQQRVQAEFTLATARAALLNAIGSQ"
     gene            complement(3100..4203)
                     /locus_tag="VFQ35_07305"
     CDS             complement(3100..4203)
                     /locus_tag="VFQ35_07305"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET9930475.1"
                     /translation="MNRLATLLEKKYLGAPLEAWLTALGVSVTVMLVLAGIRRLASRR
                     FAQWAEKTPTALDDIVLSVLSRTHGVFYLGVALWAGSQYVDLSTKAERWLRTGVSLVV
                     LFQVGIWLQVAIQRAAETWARNTRDDSDSRMMATAVAFITKLVIWAVVFVMGMSALGF
                     EISALVAGLGVGGVAAALAVQSLLSDLIASLSMFFDRPFDLGDFIVVGTEQGTVERIG
                     LRSTRLRALGGEEIVFSNGDLIKSRIRNYKRMQERRVAFTLGFDYATSPEQLEKIPDI
                     VKRIIESRQGVRFDRAHFREYGPVSLEFEVVFFVVSADMQVYLDHRHAINVEILKELR
                     KLGVTLVHMPNAKSSGDAAVSEAARLPAPTAST"
     gene            4321..5673
                     /locus_tag="VFQ35_07310"
     CDS             4321..5673
                     /locus_tag="VFQ35_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9930476.1"
                     /translation="MIRIRSSISGSMLLLALACSHAEPEANNGNATGGSTSVAKGGAS
                     TAQGGQTSSSGGTTAQGGASSSGGNSQGGASSGGTSASSGGASPGGNGSGGTTTSSSG
                     GAGGGSTAGNGGSAGAPDPKWKAAVSAQVTDQMLTDGYTAWKSKYTKTCDNGSAVVIM
                     NDSVVSEGIGYGMLLSANLNDRTFFDGLFKYYNDHLDSNGLMNWKTGVCEGPGNNNQN
                     AASDAELDVTMALIQAHARWSDGGYLDKAKNLAGKIVKFETEDCNGRKVLRPGDKFGG
                     CSDSGQPRINPSYFAVGYYRVFAAKFSDQASTWNALVNGSYDLFAIYQGRMSGLVPDW
                     SRPDGSDWNGSKYSWDACRTPWRVMIDYAYTGEPKARTFMQNVRTWFDANSSTLTEEQ
                     TNSAFHGAFALAAAYDQAKFDAAMTTWLNVKGDDVPYFQATLRLLYLLAAAGKFPSTM
                     "
BASE COUNT         1113 a         2012 c         1923 g          964 t
ORIGIN      
        1 ggccgccaag cggacgaggc gcgccgacgc ggaggagcgc gcggcggagg cgctgcggca
       61 ggtcggcctg ctgcgctggg ccggacggta tcccggcgag ctctcgggcg gccagcagca
      121 gcgggtcgcg atcgcgcggg cgctcgtcgg ctcgccgcgg ctcatcttgg cggacgagcc
      181 cacgggcaac ctcgactcgc ggacgagcct cgaggtgatg gcgctcttcc aagagctcgg
      241 ccgttctggc atcaccatcg tgctcgtcac gcacgagccc gacatcgcga cgtacgcctc
      301 gcgcgtgatc gtcttgaaag acgggctcgt cgaagcggat cgaacccaag tgcccgcaga
      361 cgcgcgcgcc gaagtcgaac ggctcgcccc cgtaccggtg tcggcatgag cctgctgcaa
      421 acgtttcgaa tcgcgtttcg agcgctcttg cgcaacaaga tgcgctcttt tttgacgacg
      481 ctcggcgtgg tcatcggggt cggcgccgtc atcgccatgg tcgccatcgg tgaaggcgcg
      541 aagtcgagcg tggagcaagc gttcgcggcg atgggcacga acctgctcat cgtcatgccg
      601 ggagcgaaca cttccggcgg cgtgcgcggc ggtttcggca gccaacccac cctcacctgg
      661 gacgatctca aggccatcca gacggagctg cccaccgtca aggcagcggc cgcttttctg
      721 cgcagcacga gctcggtggt gagcgaagac caaaactgga cgacgcagat cggcggcacc
      781 tcgcccgagt acctcgagat ccgcagctgg cccatcgctc aggggcgggc tttcacgcaa
      841 tcggacgtgg agtcctcgac caaagtggcg ctgctcgggc gcaccgtggc cgacaagctc
      901 tacgggccga gctcgaaccc cgtggggcaa ctcgttcgca tcaacaacgt acccttcgag
      961 gtgatcgggc tcctcgcgac gaaggggcag tccgcaatcg ggcaggacta cgacgacacc
     1021 gtcctcgtgc cgtactcgac gtttcgctcg aagattcagg gcggcctcaa gaactacctg
     1081 agcggcacca tcttcgttca gggcacttca caagcggagc ttcaacgcac gcaagacgcc
     1141 gtcacctcgt tgctgcgcga ccggcatcgc ctggctcaag gtgtcgacga cgacttttcg
     1201 atccgcaact tgacggagat ggcgagcgct cagcagcaag gaaccgaaac catgaccacc
     1261 ctgctcgcga gcatcgcgtt cgtttcgctg ctcgtcggcg gcatcggcat catgaacatc
     1321 atgctggtga gcgtgaccga gcgaacacgc gaaattggtc tgcgcatggc gatcggcgcg
     1381 aagcctcacc aaatcctcgc gcagtttttg gtggaggcgt tcaccctctc gtcgatgggg
     1441 ggcgcgctcg gcgtgatgtt gggcgtgggc atcgcgctct acctcgcatc gcgctttggt
     1501 tggccgctct tgatccgccc cgacatcatc gctgtctcgg tgctattcag cgctctcgtt
     1561 ggggtctgct tcgggctcta ccccgcctac aaagcgtcgc gactcgatcc aatcgatgcc
     1621 ctgaggtacg aatgaagcgc tccctattcg cgcgatcgct cggcttttgc gcggccgttt
     1681 cgatgttttt ttcgtttggc gccgctcgag cccaagaaaa agcgaacccg agccccgtga
     1741 ctggtccgac gcccgcgccg gcgccgaacg cgcccaccgc gccgaacgcg gctccggtag
     1801 acacgggcaa gttgtcgacg tcccccgcca cgaccacgac cgagctcccg gcggcgggcc
     1861 cggtgctccc gctcgcacaa gccctgcgca acgggctcga gcgccatccg acgctacgcc
     1921 aagcagaagc caattcgcgc gccgccgatg cgcgcgtgga ccaagcgttc gcgccgctgt
     1981 tgccgcaagt cacggccact gccacgtact cgcgcagtac cgcgaaccct gtgccccgcg
     2041 ccggtcaggt caacctgccg ccgcgcgatc cgagcttcga tacgtacggc ttctggaact
     2101 tcggcatcaa cgcctcctat gtcgtatacg actttggtca gacacgcggt cgcttgagga
     2161 gcgcggaggc gaacgccgag gtgacgaagc tctcggagcg cgcgcagcga ctcaacatcg
     2221 ccctcaacgt gcgcaatgcg tacttccagg cacgacaaca gaaggcgctt ttgctcgtcg
     2281 cgaccgagac gctgacgaac caggagcggc acctcggcca gattcgcggc ttcgtcgaga
     2341 ttgggacacg tccgcgcatc gacgtcgttc aggcccaagc ggatcgtgaa agcgccgagc
     2401 tccagcgcgt gaacgcggag aacaactacg cgatcgccaa ggcgcagctc gtgcaagcga
     2461 tgggcctcga caacgcgcca cccttcgagg tgagcgatga gacgctcgcc cccctcgacc
     2521 tcgaggaagc cgaaccgaac cgtctctact cccgcgcgct cgcgacccgc cccgacgttc
     2581 tcgccgcggt cgggagcgta cgcgcccaag agctctccga gagcgcgctg aaaggcggat
     2641 acgggccctc gctttcagtc tcgacgggcg tcaccgaggc cggcgctgcg ctcgacaccc
     2701 tgcgctggaa ctggaacgcg caggcggcgc taagctggcc gatctttcaa ggcggcatca
     2761 ccaaggccca ggtgcgcgaa gcgcgcgcca acgtcgacgc tgcgaccgcc caggtgaatt
     2821 tggtgaagca acaggtgctc ctcgcggtcg agcaagcgcg catcgcggtc gtcggtgcca
     2881 agcgcgcact cgccaccgcc gacgtgctcg tgcaaaacgc gcgcgagcgc ctgaacctgg
     2941 ccgagggacg ttatcagacc ggcgtcggca gcatcatcga gctcggcgac gcgcagctcg
     3001 cgttgacgaa cgcccaacag cagcgcgtcc aagcggagtt cacgctcgcg accgcgcgcg
     3061 ccgccctgct caacgccatc ggctcgcagt aagggaaggt caagtcgacg cggtgggggc
     3121 cgggagccgc gccgcctcgc taacggcagc gtcgcccgac gacttcgcgt tcggcatgtg
     3181 cacgagcgta acgccgagct tgcggagctc tttcaagatc tcgacgttga tggcgtgccg
     3241 gtggtcgagg tacacctgca tgtccgccga gacgacgaag aagacgacct cgaactcgag
     3301 cgagacgggg ccatattccc ggaagtgcgc ccgatcgaag cggacacctt gtcggctctc
     3361 gatgatccgc ttgacgatgt cggggatctt ttcgagctgc tcggggctcg tcgcgtagtc
     3421 gaagccgagc gtgaacgcga cccgccgctc ctgcatgcgc ttgtagtttc ggatgcggct
     3481 cttgatgagg tccccattcg aaaagacgat ctcttccccg ccgagcgcgc gtaggcgagt
     3541 gctccggaga ccgatgcgtt ccaccgtccc ctgctccgtg ccgaccacga tgaagtcacc
     3601 gaggtcgaag ggacggtcga agaacatcga gagcgacgcg atcaggtcgg agagcaggct
     3661 ctgcacggcg agggccgccg ccacgccacc gacgcccaaa ccagccacca acgcgctgat
     3721 ctcgaagccc agcgccgaca tgcccatcac gaacaccacc gcccaaatca cgagcttcgt
     3781 gatgaacgcg acggccgtcg ccatcatccg cgagtcgctg tcgtcgcgag tgttgcgcgc
     3841 ccaggtctcg gctgcgcgct ggatcgcgac ttgcagccaa atcccaacct ggaagaggac
     3901 gacgagggag acgccggtgc gaagccaacg ctcggccttg gtggaaagat ccacgtattg
     3961 cgagcccgcc cagagcgcca cacccagata gaagacgccg tgcgtgcggc tgagcaccga
     4021 gagcacgata tcgtcgaggg cggttggggt cttctccgcc cactgagcga atctgcgcga
     4081 tgcgagacgc cgaattcccg ccaaaaccag catcaccgtg acagaaacgc cgagcgccgt
     4141 gagccacgcc tcgagcggcg ccccgagata cttcttctcc agaagcgtcg cgagcctgtt
     4201 catgtttggc gtataccatc gagctcactg aaactgggta gcgcgtggca ttttagcggg
     4261 ttggcgttcg ccgaagccgg tgtagcctcc ctcgggaccc ctgcggagac tcactaacat
     4321 atgattcgta ttcgctcttc gatcagcggc tcgatgctct tgctggcgct tgcttgttcg
     4381 cacgccgagc ccgaggcgaa caacgggaac gcgactggcg ggagtacctc ggtcgcaaaa
     4441 ggcggagcat cgactgctca gggcggtcag acgtcgtcga gcggcggaac gacggcccag
     4501 ggcggcgcga gctcgagcgg tggtaactcg cagggcggcg cctccagcgg cggaacgagc
     4561 gcgtcgagcg gcggcgccag tccgggcggc aacgggagcg gcggaacgac gacatcctcg
     4621 agcggcggcg ccggaggagg atccacggca gggaatggcg gtagcgccgg cgcgcccgac
     4681 ccgaagtgga aggccgccgt ttcggcccag gtcaccgacc agatgctgac cgacggctac
     4741 acggcgtgga agagcaagta cacgaagacc tgcgacaacg gcagcgccgt cgtcatcatg
     4801 aacgacagcg tcgtttccga aggcatcggc tacggcatgc tgctctcggc gaacctcaac
     4861 gaccgcacct tcttcgacgg cctgttcaag tattacaacg atcacctcga ctcgaacggc
     4921 ctgatgaact ggaagactgg tgtctgcgag gggccgggca acaacaacca aaacgccgcg
     4981 agcgacgcgg agctcgacgt caccatggcg ctcattcaag cgcacgcgcg ctggtcggac
     5041 ggcggctacc tcgacaaggc gaagaacctc gccggtaaga tcgtcaagtt cgagacggag
     5101 gactgcaacg gtcggaaggt gctgcggccc ggcgacaagt tcggtggctg cagcgactcg
     5161 ggtcaaccgc gcatcaatcc gtcgtatttt gcggtcggct attaccgcgt gttcgcggct
     5221 aagttcagcg accaggcgtc gacttggaac gcgctcgtca acggcagcta cgatctcttc
     5281 gccatctacc agggcagaat gagcgggttg gtgcccgact ggagtcgccc cgacggcagt
     5341 gattggaacg gctccaagta ttcctgggat gcgtgccgca cgccctggcg cgtgatgatc
     5401 gactacgcct acaccggtga gccgaaggcg cgcaccttca tgcaaaacgt gcgcacctgg
     5461 ttcgacgcga actcgagcac cttgaccgaa gagcagacga acagcgcctt ccacggcgcc
     5521 ttcgcgctcg cggccgccta cgatcaggcg aagttcgatg ccgcgatgac gacctggctc
     5581 aacgtcaagg gcgacgacgt gccctacttc caggccacgc tgcgcctgct ctacctgctc
     5641 gctgcggccg gcaagttccc gtcgacgatg tgagcgtcag ggggatgctc ctcgacaact
     5701 tgcccgctcg acctggcgcg ccgggatccc tggcgaacat ccacgcttag attctcgtta
     5761 cgcggtcccg gcaatgggga cttcgttggg cccagcgaag catcgtgcga ggcagtgctc
     5821 tgcggaggag ccgtcacccc gagctcggca cgaaccgggc gcgctcgcct aaagtgcgcc
     5881 tcagaatgga gccggaccta aaagtggagc cgggcctaac gatggaggtg tcttcgccgt
     5941 tctaaaaacc ttgttctccg cgctgatcgg cgccttcagc cagttgtcgc ccctgtgtct
     6001 accgcgcacg ct
//
LOCUS       DASTKS010000354         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_279431, whole genome shotgun sequence.
ACCESSION   DASTKS010000354 DASTKS010000000
VERSION     DASTKS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279431"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VFQ35_07315"
     CDS             complement(<1..1944)
                     /locus_tag="VFQ35_07315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930477.1"
                     /translation="MRSGAPSSESEAKRSDAPARVSAEPNATLALGRILSERPSDGAP
                     GRMSFPTLDVVSGVPANRGSFTTLDAASNAPAKRASYATLDQALGTAATAPLGGAHDP
                     RSLLMHLPAAELGVTHEHSGRYAAEANSELGRGGIGRVFVSLDKHLGRRVAVKELLLD
                     GSDDPARPGMLQTVARFLREARITGQLDHPNIVPVYELGRRADGSLYYTMRVVRGRTL
                     SRALAEAKTLPKRLELLGHFIGLCQAIAYAHSRGVVHRDIKPDNVMIGEFGETVVLDW
                     GMAKANGDDADRTSVLPVRDPLRLDLTVDGSLCGTPTHMSPEQARGAIQEVDARSDVW
                     ALGVVLYSLLAGRNPFEGKTLAELIHAILKGKYAPLASVDPAIPAELAAIAERALRAA
                     PEQRYESARELLRDVEAYRSGARVGAYAYSTLDLLRRFLERHRTAAVASGVGLGVALV
                     LAVASYVRVAAARDRALVAEQRATQNERQARASERAAKQSLSYVLVEKAQQAMSEGDR
                     TSAELLAASALELAERSEARGIAVTSESTFRPEPKLVHRAAAGCKVSAVSYAGSSIAC
                     ARGEEVTLYDSRDGESRAVLRARGNVVALDLSLDGWHLVIAHEGGVVSVFSLTGGGAR
                     LLHERSVGSTTRVAVSSNGAYVAC"
     gene            complement(2097..2306)
                     /locus_tag="VFQ35_07320"
     CDS             complement(2097..2306)
                     /locus_tag="VFQ35_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930478.1"
                     /translation="MKVRMLQQYFLAASAAVATGPGIPGPNPPGQPTPPERQAPDPFD
                     PPTPNYEDEPPVTPIDDPPPDERAF"
     gene            2363..2818
                     /locus_tag="VFQ35_07325"
     CDS             2363..2818
                     /locus_tag="VFQ35_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930479.1"
                     /translation="MGKSLARIGFRAAALLPIAAITACSGRAPANQSSTRSDASARFP
                     ADVIAIREAADAYLKTHRAEALSHVPADWAPLVPDHAGEPTESADPGYLLPPWRLRLD
                     RNRAALRYTPREGVTEHDRFWFEIGLEKTAETWRISGDGLVFGHAWRRE"
BASE COUNT          452 a          941 c          979 g          468 t
ORIGIN      
        1 gcaggcgacg tacgcgccgt tgctcgacac cgccacgcgt gtcgtcgagc cgacgctgcg
       61 ctcgtgaagt agccgagcgc cgccgccggt caacgagaac acgctgacga ctccgccctc
      121 gtgcgcgatg acgaggtgcc aaccgtcgag cgagaggtcc agcgcgacca cgttgccgcg
      181 cgcacgcaga accgcgcggc tctcgccgtc gcgcgaatcg tacaaggtga cttcttcgcc
      241 gcgcgcgcaa gcgatcgacg agcccgcgta gctcacggcg ctcactttgc atccggcggc
      301 ggcgcgatgg acgagctttg gttcgggccg aaacgtcgac tcgctggtca cggcgatgcc
      361 ccgagcttcg gagcgctcgg cgagttcgag cgcgctcgcg gccaagagct ccgcgctcgt
      421 gcgatcgccc tcgctcatcg cttgctgtgc cttctcgacc agcacgtaac tcagactctg
      481 cttcgccgcg cgttcgctgg ctcgcgcctg gcgttcgttc tgagtcgcgc gctgctctgc
      541 cacgagcgcg cgatcgcgag cggccgcgac gcgaacgtac gaggcgaccg cgagcaccag
      601 cgcgaccccg agcccgacgc cggaagctac ggccgcggtg cggtgccgct ccaagaagcg
      661 tcgcagcaga tcgagcgtcg agtaagcgta ggcgccgaca cgagcgccgc tccgataagc
      721 ttccacgtca cgcaagagct cgcgagcgct ttcgtagcgt tgctctggag ccgctctcaa
      781 ggcgcgttcg gcgatggcgg cgagctccgc gggaatcgca gggtcgacgc tcgcgagagg
      841 cgcatatttg cccttcagaa tagcgtgaat cagctcggcg agggttttgc cctcgaacgg
      901 atttctgccc gccaagagcg aatacaaaac gacgccgagt gcccagacgt ccgagcgtgc
      961 atcgacttct tgaattgcgc cgcgtgcttg ctcggggctc atgtgcgtcg gcgtgccgca
     1021 caggctaccg tcgacggtga ggtcgagccg cagtgggtcg cgcactggga gcacactcgt
     1081 gcggtcggcg tcgtcgccgt tcgccttcgc catgccccag tcgagcacga ctgtctcgcc
     1141 gaactcgccg atcatgacgt tgtccggctt gatgtcccga tgaacgacgc cgcggctgtg
     1201 agcgtacgcg atcgcctggc agagcccgat gaagtgcccg aggagctcga ggcgcttcgg
     1261 cagcgtcttc gcctcggcca gcgctcgcga tagggtgcgg ccgcggacga cccgcatcgt
     1321 gtaatagagg gacccgtcgg cgcgccttcc gagctcgtaa acggggacga tgttgggatg
     1381 atcgagctgc ccggtgatgc gcgcttctcg caggaaacgc gcgacggttt gcagcatgcc
     1441 cggccgagcg gggtcgtctg aaccgtcgag caagagctcc tttacggcga ctcgtcgccc
     1501 gaggtgcttg tcgagcgaca cgaacacgcg cccaatgcct ccgcgcccga gctcggagtt
     1561 ggcttcggcc gcgtaacgtc ccgagtgctc gtgcgtgacg ccgagctccg cggcgggcag
     1621 atgcatcagc aaactgcgcg gatcgtgcgc gcccccgagg ggcgccgtcg ccgccgtgcc
     1681 gagcgcctga tcgagcgtcg cgtagctcgc gcgtttggcg ggcgcgttgc ttgcggcgtc
     1741 caaagtggtg aaacttccgc gattggcggg gacaccgctc accacatcca gtgtcggaaa
     1801 gctcatgcgc ccgggcgcac cgtccgacgg gcgctccgaa agaatccgac cgagcgcgag
     1861 cgttgcgttc ggctctgcgg agacacgcgc tggcgcgtcg gagcgctttg cctcggattc
     1921 cgacgaaggc gcgcccgatc gcacggttcg aatgtctccg cgaccgctca tccgctgaac
     1981 gagaacggac ggtcgaggca ggcgtttatc gcgtaccttg ccctacgcgg agactgcgcc
     2041 ggaaacgggg tcgtgtcgct ccgccgcatg tggcgatcgc gagcacgccg ccgcggctaa
     2101 aacgcgcgct cgtccggcgg cggatcgtcg atcggcgtaa ccggcggctc gtcttcgtag
     2161 ttgggcgtcg gagggtcgaa cgggtccggc gcctgacgct cgggaggggt cggctgtccc
     2221 ggcggattgg gtccagggat gccaggaccg gttgcgacag cggcactggc ggcgaggaaa
     2281 tattgctgga gcatgcggac cttcactgca tctcccgtgc cgccctggcg aacggagagc
     2341 gctgtgcgta ctctgtcgag ccgtggggaa atcgctggcg cgaataggtt tccgggcagc
     2401 ggcgttgctc ccgatcgccg caatcactgc gtgcagcggt cgggcgcccg caaatcagtc
     2461 atcgacgagg agcgacgcga gcgcgcgctt tcccgccgat gtcatcgcga ttcgcgaggc
     2521 cgctgacgcg tacttgaaga cgcatcgggc cgaggcgctg agccacgtgc ccgcggactg
     2581 ggcacctctc gttcccgatc acgccggtga gccgacagaa agtgcggatc ccggatacct
     2641 cttgcctcct tggcgactgc ggctggacag aaatcgcgcg gcactgcgtt acacgccacg
     2701 tgagggagtg actgagcacg ataggttttg gttcgagata ggcctcgaga agaccgccga
     2761 gacctggcgc atttcggggg acggcctggt tttcggtcac gcctggcgac gcgagtgacc
     2821 gaccgcgaca tctacgaccc
//
LOCUS       DASTKS010000355         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_504372, whole genome shotgun sequence.
ACCESSION   DASTKS010000355 DASTKS010000000
VERSION     DASTKS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504372"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="VFQ35_07330"
     CDS             <1..1584
                     /locus_tag="VFQ35_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET9930480.1"
                     /translation="GASIPIALLMGMYIYGVRKGSTRGIREASLVGVFLLFLALIGGK
                     AVADSGAADAFRFSKTTLTLAIAGYGFLASVLPVWLLLCPRDYLSSYLKIGTILLLVG
                     GILIVNPPVQMPGVTQYVNGGGPIIKGPLFPFVFITIACGAISGFHALVASGTTPKMV
                     DRESHCRPIGYGSMLMEGLVGITALIAACVMPPEDYVAINTDPKIAMVATPATGQQGL
                     ARSRAELARADAALTAHDRQILGLSAGESVTRLAEARLPASKLLSLSNRSLAALGYEV
                     DPAEKHAIGLGDADFARLGIHVKELPGLSRGAGEVVAARTGGGVSLAVGMARVFSGLP
                     GMKTLLAYWYHFAIMFEALFILTTIDTGTRIGRFLLQEFLARFNPELGRANSLPSSVF
                     ATLVIVAGWTYFILTGNISTIWPMFGIANQLLACTALCVGTTIILRDSPRKKYALVTF
                     LPLVFISVTTITAGVQSLFKLYYPMTQVAATRTTGLVNLLVTSTLLVGVSMIMVGSAR
                     RWLLMLRSDAPKSATPEPA"
     gene            1636..2028
                     /locus_tag="VFQ35_07335"
     CDS             1636..2028
                     /locus_tag="VFQ35_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9930481.1"
                     /translation="MSHAIDHIGFSVKDYERSKAFYTQALAPLGLTLVMEFGKAGGFG
                     RGKPELWFGESQMDFQTAAQVAVITPLHVCLTARSREEVDAFYKAALAAGGKDNGAPG
                     IRAHYHPNYYGAFVIDPDGHNVEAVFHG"
     gene            complement(2115..2873)
                     /locus_tag="VFQ35_07340"
     CDS             complement(2115..2873)
                     /locus_tag="VFQ35_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9930482.1"
                     /translation="MSQGDRDSNPDVVRLPWPEVGPVVLKTRGLCRELGTDVKNRVLI
                     DVDLEVRAREFVSITGFSGSGKSTLLYLLGALDRPTSGVVELDGVDISKLEDDARAHL
                     RNEKLGFVFQFHFLLPEFTVLENVMIPMLRRADKSRRDIEQKARDVLASIGLGDLLGR
                     RPHQLSGGQQQRVSIARAVANDPRIVLADEPTGNLDSKSGRNVMDVFERLVRDQGITV
                     IMVTHEQTFAARTSRQLSMSDGRIVTDVDQRPKP"
     gene            complement(2870..>3241)
                     /locus_tag="VFQ35_07345"
     CDS             complement(2870..>3241)
                     /locus_tag="VFQ35_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9930483.1"
                     /translation="GFGILAIQVMIVLQKTRDIAILRSVGFRRGDILVGFLLQGAVIA
                     LVGALVGDLIGHYLLIALSHLKTHQEGLVKSEYFLVTDDPRFYWYGTGFALAVGLVAS
                     LIPAVRGSRVEPVDVLRGQLG"
BASE COUNT          551 a         1039 c         1035 g          616 t
ORIGIN      
        1 ggcgcttcga tccccatcgc gctcttgatg ggcatgtaca tctacggagt ccgaaagggg
       61 agcacgagag gtattcgcga ggcgagcctg gtcggagtat ttcttctgtt cctggcgctg
      121 atcggcggga aagcagtggc cgattccggg gcggccgacg ccttccggtt ctccaaaacg
      181 accctcactc tcgccatcgc cgggtacgga tttttggcga gcgtgctgcc ggtgtggctg
      241 ctcctgtgtc cgcgcgacta cctgtcgagc tacctcaaga tcgggacgat cctgctgctc
      301 gtcggcggca tcctcatcgt gaacccaccc gtacagatgc caggggtcac ccagtacgtg
      361 aacggtggtg gacccatcat caagggaccg ttgttcccct tcgtattcat cacgattgcg
      421 tgcggcgcga tcagcgggtt ccacgcgctc gtcgcgagcg gcacgacccc caagatggtg
      481 gaccgtgaga gccattgtcg tcccatcggc tacggctcga tgctgatgga gggcctggtc
      541 gggatcacgg ctctcatcgc agcttgcgtc atgccgcccg aggactacgt cgcgatcaac
      601 accgatccta agatcgcgat ggtggcgacg ccggcgacgg ggcagcaggg cctcgcgcgc
      661 tcacgcgcag agctggctcg agccgacgcc gcgctcaccg cccatgaccg ccaaatcttg
      721 ggcttgagcg ccggcgaatc ggtgactcga ttggcggagg cgcggcttcc cgcgtcgaag
      781 ctgctctctc tttcgaaccg gtcgctcgcc gcgctcgggt atgaagtcga tccagccgag
      841 aaacacgcca tcgggctcgg agacgccgac ttcgcgcggc tcggcatcca cgtgaaggag
      901 cttcccggcc tctcgcgcgg cgcgggcgag gtggtcgcag cgcgcactgg cggaggcgtt
      961 tcgctcgcgg tcggaatggc gcgcgtgttc agcgggctgc cagggatgaa gacgctgctc
     1021 gcctattggt accacttcgc gatcatgttc gaggcgctgt tcatcctcac cacgatcgac
     1081 acggggacgc gcatcggccg cttcttgctg caggagttcc tcgctcgctt caacccggag
     1141 ctcggtcggg cgaacagctt gccctcttcc gtcttcgcga ctctggtgat cgtcgccggc
     1201 tggacctact tcatcctgac cggtaacatt tccacgatat ggccgatgtt cggcatcgcc
     1261 aatcaactcc tggcgtgcac cgcgctgtgc gttgggacga ccatcatctt gcgcgactcg
     1321 ccgcgtaaga agtacgcgct ggttaccttc ttgccgctcg tgttcatcag cgtgacgacg
     1381 attaccgccg gtgtgcagag cctcttcaag ctctactacc cgatgacgca agtcgcggcg
     1441 acgcgcacga ccggtctcgt caacttgctc gtgacgagca cgttgctcgt tggggtcagc
     1501 atgatcatgg tcggcagcgc gcgtcgctgg ctcctgatgc tccgttcgga cgcgccgaag
     1561 tcagcgacgc ccgagccggc gtgaagctcg agtttggcgt cgcctagccg cgggagcact
     1621 gctagcttcg gccccatgag tcacgcgatc gaccacattg gcttctccgt gaaggactac
     1681 gagcgttcga aggcgtttta cacgcaggcg ctggcgcccc tcggcctcac cctcgtgatg
     1741 gagttcggca aggccggtgg cttcggtcgt ggcaagcccg agctttggtt cggtgaaagc
     1801 caaatggact ttcagaccgc cgcgcaggtc gcagtcatca ctccgcttca cgtctgtttg
     1861 accgcgcgga gccgcgagga ggtcgacgcg ttctacaagg cagcgctcgc ggcgggaggc
     1921 aaggacaacg gcgctcccgg tattcgagcg cactatcacc cgaactacta cggcgccttc
     1981 gtgatcgatc ccgacgggca caacgtcgaa gctgtcttcc acggctgaga actcgcgttt
     2041 gccgcggccg gcgcgcagcg gtgccagctc gaaccgaaaa agcctgcagt tgcgagctcg
     2101 agccgagccg gcggtcaggg cttggggcgc tggtcgacat ccgtgacgat gcggccgtcg
     2161 ctcatcgaaa gctggcgcga cgtgcgcgcc gcgaacgtct gctcgtgcgt gaccatgatt
     2221 acggtaatac cctggtcccg cactagccgc tcgaacacgt ccattacgtt gcgcccgctc
     2281 ttcgagtcga ggttccccgt cggctcgtcg gcaagcacga tgcgcggatc gttcgctacc
     2341 gcgcgcgcga tactgacgcg ctgttgctgc ccgcccgaga gttggtgtgg gcgccgcccg
     2401 agcaagtcac cgaggccaat cgaggcgagc acgtctcgcg ctttttgctc gatgtcacgg
     2461 cggctcttgt ctgcgcgtcg cagcatcggg atcatgacgt tctcgaggac ggtgaactcc
     2521 ggcaagagga agtgaaactg aaatacgaag ccgagcttct cgttccggag gtgagcgcgc
     2581 gcgtcgtctt cgagcttgga aatgtcgacg ccgtcgagct ccacgacgcc ggaggtcggt
     2641 cggtcgagcg cgccgagcag gtagagcagt gtgctcttgc ccgagccgct aaagccggtg
     2701 atgctcacga attctcgcgc tcggacctcg aggtctacgt cgatgagcac gcggttcttc
     2761 acgtcggttc cgagctcgcg gcagagccct cgcgtcttca gtacgactgg gccgacttcg
     2821 ggccaaggca accgaacgac gtcggggttc gagtctcggt cgccctggct catccgagct
     2881 gacccctgag cacgtcgacg ggttccacgc gcgagccgcg cacggctgga atcaggcttg
     2941 cgacgagccc gaccgcgagc gcgaagccgg tgccgtacca ataaaaacgc gggtcgtcgg
     3001 ttacgaggaa gtattcgctt ttgacgaggc cctcttggtg agtcttcaag tgcgagaggg
     3061 cgatgagcaa gtagtggccg atgagatctc cgaccaaggc gcccacgagc gcgatcacgg
     3121 cgccttgcag cagaaagccg accagaatgt cgccacgccg aaagcccacg ctgcgtaaga
     3181 tcgcgatgtc gcgtgtcttc tgcaatacga tcatcacctg gatcgccaaa ataccgaagc
     3241 c
//
LOCUS       DASTKS010000356         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1397220, whole genome shotgun sequence.
ACCESSION   DASTKS010000356 DASTKS010000000
VERSION     DASTKS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397220"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..831)
                     /locus_tag="VFQ35_07350"
     CDS             complement(196..831)
                     /locus_tag="VFQ35_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930484.1"
                     /translation="MLSGTCLVAASLLSRPASAQSNGEQDGYLRVAVGPSFLASELSL
                     RTYQSWSQYGAGASAEMAVGARLSPRAVLGGFLSFTGLPSLHRARDDESQSMDLNFSA
                     LGLLGPFVDIDLNPTAPTPLYLELGLAAALLSQGGGKFPFSSSSRTLRGGVANVGLRL
                     QLARAGRVSWDLSGRLSAGAVTRDELGYSDGATRATVVLLMPSVLVSLVYR"
     gene            complement(842..1315)
                     /locus_tag="VFQ35_07355"
     CDS             complement(842..1315)
                     /locus_tag="VFQ35_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930485.1"
                     /translation="MQLLASVLWGRDFDEGGERPLGLGFGGRVGYTLDNAVYLGARYE
                     WFLGATNEEAGGTHSWRIGQLALEAGRDIGLGREFVLRPSIAAGLAYRADKCHGVYCD
                     SGGGTKFGLTAGPRLGVAWHHGMLVVATDAGLAVFFLEPTAVPGFELGVHGGVAF"
     gene            complement(1581..2252)
                     /locus_tag="VFQ35_07360"
     CDS             complement(1581..2252)
                     /locus_tag="VFQ35_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9930486.1"
                     /translation="MTLRAPLRAVLFDLDGVLLDTEPLYTQGIQEVVAEFGHVYDWSL
                     KRDMMGRSDLEGAELVVSRLALPISPAEYMARREPIMERLLATSPVMHGARELVEELA
                     LRGVPLALATSSRTRLYDIKAKPHAWLGAFKHIVCGDDPEVQQLKPAPDIFLVAARRL
                     GVASEFCAVVEDSPAGVLAAQRAGMQVAALPDPALDSDIVRTADLVATSHAEIRSALF
                     AALAV"
     gene            complement(2303..>2655)
                     /locus_tag="VFQ35_07365"
     CDS             complement(2303..>2655)
                     /locus_tag="VFQ35_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930487.1"
                     /translation="NAELSLRLSPRAVQADIKIGPPRARWPQDNVIVSVRLSDARGRP
                     LGDDVAVKPIAYVNVTPVKVEWKRDHNVLHAVIPPSSEPGPWVVRVEVTDDTGALAGR
                     DFLEVAPAAIARSE"
BASE COUNT          503 a          889 c          853 g          410 t
ORIGIN      
        1 cgtatcgttg gcgaaatgcc gggggtcccc atcgctggag agcttcccgc cgcggtcgct
       61 tgaccctgcg gtggcgacgt ctacgagcct agcgagcgcg ttcgcgagct tcgggggctg
      121 agcgtctcac tacgaaaggc gggctcgttg agtcgctctc gcctcccgcg acttgcgagc
      181 ccccgctgaa tcgggtcagc gataaacgag cgaaacgagc acgcttggca tcaacaggac
      241 tacggtcgct cgtgtcgctc cgtccgagta gcccagttcg tcacgcgtga cggcgccggc
      301 ggagagcctt ccgctgagat cccagctgac cctccccgcg cgggcgagct gcaagcgaag
      361 tccgacgttc gcgacgccgc cgcgcagagt cctcgacgag ctcgaaaacg ggaattttcc
      421 tccgccctgt gacagaagcg cggccgcgag cccgagctcc aggtaaaggg gagtgggcgc
      481 agtgggattg aggtcgatgt cgacgaatgg gcccagcaga cccaacgccg agaaattcaa
      541 atccatcgac tgggattcgt cgtcgcgcgc gcggtgtagc gatggaagtc cggtgaacga
      601 caagaagcct cccagcacgg cgcggggcga gagccgcgct ccgaccgcca tttcggcgga
      661 ggcccccgca ccgtactgcg accacgattg gtaagtgcgc agcgacagtt cgctcgccag
      721 aaacgacggc ccgaccgcga cgcgcagata accatcctgc tcaccgttgc tctgcgccga
      781 cgccggacgg ctcagcagcg aggcggcgac gaggcatgtc cccgacagca cgaccgacgc
      841 tctaaaacgc gacaccaccg tgaactccca attcgaagcc aggcaccgct gtcggttcca
      901 agaagaacac agcgagccca gcatccgtcg cgaccaccaa catcccatgg tgccacgcaa
      961 cccccagccg cggtcccgcg gtcaagccga acttggtacc gcctcccgag tcgcagtaca
     1021 cgccgtggca tttgtctgcg cggtacgcga gccccgcggc gatcgacggt cgcaacacga
     1081 actcgcgtcc gaggccgatg tcgcggccag cctcgagcgc cagctgaccg atacgccacg
     1141 aatgagtgcc gcccgcttcc tcgttcgtag cgccgaggaa ccactcgtac cgagctccca
     1201 agtagaccgc gttgtcgagc gtgtagccga ctcgcccgcc gaaacccaac ccgagtgggc
     1261 gctcgccacc ttcgtcgaag tctcgccccc acaatacgga agcgagtagc tgcactcgcc
     1321 ctacgcttgg cgctgcatca ctcgcgcacg cgcggcgagc gatgaggcca atggcaaaag
     1381 ctgcgagcac acccagaatt cgcacgcccc gacgttctcc gagctaaccc tatatcgcag
     1441 acgtaaggga ttggggactc gctctctgcc taggtgtgcg aattgtccgt ccggacgggg
     1501 gactgagcgg atttgcttgg tgagctgacg tcagctggcc agcgttctat cgggcaagtt
     1561 tcgccagtga gacaagggag tcagacggcg agcgccgcga acaacgcgga ccggatttcg
     1621 gcgtgcgagg tcgcgaccaa gtccgctgtg cgcacgatgt cgctgtcgag cgcggggtcc
     1681 gggagcgccg cgacttgcat cccggcgcgc tgagccgcga gcaccccggc gggagagtct
     1741 tcgacgactg cgcaaaactc gctcgccaca ccgaggcgtc ttgccgcgac caaaaagatg
     1801 tccggcgcgg gcttgagctg ctgaacttcc gggtcgtcgc cgcacacgat gtgcttgaac
     1861 gcgccgagcc aagcgtgagg tttcgccttg atgtcgtaga ggcgcgtacg gctgctcgta
     1921 gcgagggcga gcggaacgcc gcgcagagcg agctcttcga ccagctcacg cgcaccatgc
     1981 atcaccggtg acgtcgcgag aagccgctcc atgatcggct cgcggcgggc catgtactcg
     2041 gccgggctga tcggtaaggc gaggcgcgaa accacgagtt cggcgccttc caaatcgctg
     2101 cggcccatca tgtcgcgctt gagcgaccag tcgtacacgt gaccgaactc ggcgacgacc
     2161 tcttggatac cttgtgtgta gagcggctcc gtgtcgagca agacgccatc gagatcgaac
     2221 agaactgcac gaagtggggc acgtagagtc acggcgcgaa gcgtctagcg tcgcgcggcc
     2281 gcaacgtaaa ggggagaagt gctcactcgc tccgcgcgat ggcggccgga gccacttcga
     2341 ggaagtcacg tcccgcgagc gcacccgtgt cgtcggtcac ttcgactcga acgacccacg
     2401 gaccaggctc cgagctcggc ggaattaccg cgtgcaacac gttgtggtcg cgcttccact
     2461 cgaccttgac cggcgtgacg ttcacgtacg cgatcggctt gaccgccacg tcgtcaccca
     2521 aggggcgccc gcgtgcgtcg ctcaggcgaa ccgaaacgat gacgttgtct tgcggccagc
     2581 gcgcacgcgg cggaccgatt ttgatgtcag cttgcacggc tcgcggggag agccgcaacg
     2641 agagctcggc gttcg
//
LOCUS       DASTKS010000357         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_280093, whole genome shotgun sequence.
ACCESSION   DASTKS010000357 DASTKS010000000
VERSION     DASTKS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280093"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VFQ35_07370"
     CDS             complement(<1..1311)
                     /locus_tag="VFQ35_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9930488.1"
                     /translation="MNSESPTLSRRSWLKTVIAGAVAAAPLGCPHAEKKEREGGSNQS
                     AARRPPAVRGENFSNGHRKRDGQAFPRVEPSETCEVVIIGGGPSGLCAMHLLEGRDAI
                     LLEKEDRFGGNCSTDSWEGIRFSTGAAFYSEGDTELVQLLASVGAPGMPVSGGDALIV
                     NGAPHFDFFGDGAQGLPFPQSVRDDFRRSRERAERLRDTHASRVLDERPFSELLKEHA
                     PEVKRFWDRFGASNWGADSEHTSARLGVQAYGWLSGEEKRLTYPGGLGVGADALANAL
                     DRKYPGQLRKNSFVHHVEFEEGKSRSVLVHTLVNGEPRTLRARAAILAVPKFFAARLV
                     PDLSEEQRAAMTGYRYAPYPVFNVCLHSKGPAPAYDNWFLDEPFADFIPADWVLNAGR
                     GAADQRSALTVYHPLPESRRSELLDDDKLVTMSEDVVLRLDRHYP"
     gene            complement(1387..2226)
                     /locus_tag="VFQ35_07375"
     CDS             complement(1387..2226)
                     /locus_tag="VFQ35_07375"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HET9930489.1"
                     /translation="MIANAYENRELYDSFAAGEIVVLSGAGVSTDSGIPDYRDRNGDW
                     KRKRPVEYRDFVGSEQTRRRYWARSLIGWPHFEKAQPNAAHRSVYELERMQLVSLVIT
                     QNVDGLHQRAGSERVLDLHGRLDTVVCLTCRRAIPRAELQAELARKNPRFLEQRARVA
                     PDGDADLDDLDPSDFELVACEACDGILKPDVVFFGENVPRPRVERAYEALSKARLLLV
                     VGSSLMVFSGYRFARTAAKNGIPIVIVNQGKTRADEVARFKFEANAGDVLESIVRGLG
                     SSR"
     gene            complement(2223..>2580)
                     /locus_tag="VFQ35_07380"
     CDS             complement(2223..>2580)
                     /locus_tag="VFQ35_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930490.1"
                     /translation="SATPVATKAARSKSTRAAIAEEVAPTKKPAPSAETGPDRENVAK
                     ALSLASASASTCSDGPERGRVAVTFAPSGSVQGVRLVQQFSEASVNSCVLRAIGRAHV
                     TPFSGDSVTVTKTLSW"
BASE COUNT          434 a          838 c          840 g          468 t
ORIGIN      
        1 ggggtagtgg cggtccagac gcagcaccac gtcctcggac atcgtgacga gcttgtcgtc
       61 gtcgagtagc tcgctgcgtc gagactcggg cagcgggtga tacacggtga gggcgctcct
      121 ttgatcggct gcgccgcgcc ccgcgttgag cacccagtcc gccggaatga agtcggcgaa
      181 tggctcgtcg aggaaccagt tgtcgtacgc gggcgccggc cccttgctgt gcaagcagac
      241 gttgaagacc gggtacggcg cgtagcgata tcccgtcatc gcggcgcgtt gctcctccga
      301 cagatccggt accaggcggg ccgcgaagaa tttggggacg gcgaggatgg cggcgcgtgc
      361 gcgcagagtt cggggctccc cgttcaccag ggtgtggacg agcacgctgc gcgacttgcc
      421 ttcttcgaac tcgacgtggt ggacgaacga gttcttgcga agctgtcccg gatattttcg
      481 gtccagggcg ttcgcgaggg cgtctgcgcc aactccgagc cctcccgggt acgtcaaacg
      541 cttctcctca ccgctgagcc agccataggc ttgtacgccg agccgcgccg aagtgtgctc
      601 gctgtcggcg ccccagttcg aagcgccgaa ccggtcccaa aagcgcttca cctcgggagc
      661 gtgctctttg agcagctcgg agaacggccg ctcgtcgagg acgcgcgaag cgtgcgtgtc
      721 gcgcagccgt tcggcacgtt cacgcgaccg tctgaaatcg tcccgcactg attgcgggaa
      781 aggcagccct tgcgcgccat ctccgaaaaa gtcgaaatgc ggtgcgccgt tgacgatcag
      841 cgcatcgccg ccgctgaccg gcatgcccgg cgctcccacc gacgccaaga gctgcacgag
      901 ctcggtgtcg ccttcgctgt agaaagccgc gccggtcgag aaacgaatcc cttcccaact
      961 atcggtcgag cagtttccgc caaagcgatc ctctttctcg agcaagatcg catcacgccc
     1021 ctcgagcagg tgcatcgcgc acagcccgct agggccgcca ccgatgatca cgacctcgca
     1081 ggtttcgctc ggttcgactc tggggaaggc ctggccgtcg cgttttcgat ggccgttgct
     1141 gaagttttct ccgcggacag cgggcggtct acgcgccgcg gactgattgc ttccgccctc
     1201 gcgctccttc ttttccgcgt gcgggcagcc gagcggtgcc gcggcgacgg cgcctgcaat
     1261 gaccgtcttc agccagctgc gccgagagag tgtgggactt tcggagttca agcgctcccc
     1321 atacaacgcg tttgcggtcc ggatgcaagg aggttgcggt cgagactgcg cgcgcagaag
     1381 ggcatctcag cgggacgagc caaggcccct gacgatgctt tccaaaacgt ccccggcgtt
     1441 cgcttcgaat ttgaagcggg cgacttcgtc ggcgcgtgtc ttgccttggt tcacgatcac
     1501 gatggggatg ccatttttcg cggcggtccg cgcgaagcga taacccgaga acaccatcag
     1561 tgacgagccc acgacgagca gcagccgagc cttactgagc gcttcatagg cgcgttctac
     1621 gcgcgggcga ggcacgttct ccccgaaaaa aaccacgtcg ggcttcaaaa tcccgtcaca
     1681 ggcctcgcat gcaacgagct cgaaatcgct cgggtccaag tcgtcgagat cggcgtcccc
     1741 gtccggtgcg acgcgcgcgc gctgctcgag gaatctcgga tttttccgcg cgagctcggc
     1801 ctgaagctcg gcgcgtggaa tcgcgcgtcg gcaggtgagg cacacgaccg tatcgaggcg
     1861 gccgtgcaaa tcgagcaccc gctcgctccc ggcacgttgg tgcaagccgt cgacattctg
     1921 agtgatgacc agcgagacca gctgcatgcg ctcgagttcg tacacgctgc gatgcgctgc
     1981 gttcggctgg gctttctcga aatggggcca gccgatcagg ctgcgagccc aatagcggcg
     2041 cctcgtctgc tccgagccga cgaaatcgcg atactcgacg gggcgcttgc gcttccaatc
     2101 gccgttgcgg tctcgataat cgggaatgcc cgagtcagtg ctcaccccgg ctccactgag
     2161 cacgacgatc tcgcccgccg caaacgagtc gtaaagctcg cggttctcgt aagcgttcgc
     2221 gatcaccagc tgagcgtttt ggttacggtg acggagtcgc ccgagaaggg agtcacgtgc
     2281 gcccttccga tcgcacggag cacgcacgag ttcacggagg cctcgctgaa ctgctgaacc
     2341 aagcgaacgc cctgcacgct accggaagga gcgaaggtca cggcgacgcg gccgcgctcg
     2401 ggcccgtcgg agcacgtgct tgccgaagcg gaggcgaggc tgagcgcctt ggctacgttc
     2461 tcgcggtccg gccccgtttc ggctgacggg gctggcttct tcgtcggagc gacctcttct
     2521 gcgatagcag cgcgcgtgct cttggagcgc gctgctttgg tcgcgaccgg tgtcgcgctg
//
LOCUS       DASTKS010000358        10137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1620221, whole genome shotgun sequence.
ACCESSION   DASTKS010000358 DASTKS010000000
VERSION     DASTKS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620221"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFQ35_07385"
     CDS             complement(<1..596)
                     /locus_tag="VFQ35_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET9930491.1"
                     /translation="MIAAAARPRRPSDLTRLLLFDPESNAYADRELSELPRILRAPDI
                     LVFNDSATLPAALRPNDTLELRLTGQAPDGSFWAITLGSGDHRQPTERRGPAPTLCVD
                     CEIAFSEQLQARVVEIDPSHPSRVRLAFTQQGAAFWAGLYRSARPIQYAYLRAELELW
                     DIQTPFASVPWSVEMPSAGKPFNWALLSALRRQGLSWAV"
     gene            complement(593..1345)
                     /locus_tag="VFQ35_07390"
     CDS             complement(593..1345)
                     /locus_tag="VFQ35_07390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9930492.1"
                     /translation="MDHSNQQAQRGYWAGRGALVTGGSRGLGAALGAELAARGARVVL
                     TARGAPELDIVVQQIRERGGEAHALAADLSNKNEIYPLTGAAAALVGPIEVLFHNAST
                     LGPLPLRLLLDTECEDLADVLELNLLAPFRLSKALAGNMAIQGEGLIVHISSDAAVTP
                     YERWGAYSVSKAALDHLSRLWAQELSEFGVDVLSIDPGEMDTRMHRDALPEAPTAELA
                     KPTEVASAILDYLERGAWQSGERVTVENWRHP"
     gene            1344..1973
                     /locus_tag="VFQ35_07395"
     CDS             1344..1973
                     /locus_tag="VFQ35_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET9930493.1"
                     /translation="MAVKLAALVSKMALLPVYWQNSSAMSDEMAQRLGQNIVQLRQSR
                     GFTQLQMAKLANLPRATWALLESGTANPTLTVLQAAAAALQVNLDELLAEPKALVQHY
                     PRGSLPSRRRGSVTVSSLLPDNIPGTQIERIELPPGARLTGVPHTFGSREYLSCESGE
                     IELVAGGTRFTLKPGDVVVFRGDQRHSYANLERSQAIGFSVVLVNRKVD"
     gene            complement(2024..2470)
                     /locus_tag="VFQ35_07400"
     CDS             complement(2024..2470)
                     /locus_tag="VFQ35_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9930494.1"
                     /translation="MKKAIIGYWAATALVALAFGFGGIMDLLRPPQMVEQMAHLGYPA
                     YLMTLLGAWKVLGVAAVLAPGLPRLKEWAYAGMVFDLTGAAVSHAAVGDSAGNILTPI
                     VLLGVVFASWALRPDSRKVGTSTRAGESVPAPAYAELANAGSSIAR"
     gene            2647..3600
                     /locus_tag="VFQ35_07405"
     CDS             2647..3600
                     /locus_tag="VFQ35_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9930495.1"
                     /translation="MKDAPETSELMAFAKTVETKSLSRAAAELGVPRATISRRLARLE
                     ELLGVRLLRRSTRSLALTEEGKAFYARTRVALQAVAEAQASVQPMADRLRGDLRVSFP
                     PIQDAAMFGLLVDFARRNPSVRLHAHFSSVHVDLRRGDYDVALRGIMESSPGLVQRTL
                     LRVPLIAVASPEYLAEHGVPRRKQDLRQHRCLLGFARGEIPESYWPVLGGGKLRVEGC
                     FFSNDLSLLCEAARQGLGIALLPRMFVDPLLSMGLLVHVLPSVIGAESRMALVYPERE
                     FMAPQVRAFIDEIARRIPEELANGSLLPQQPRPPSRLGRKR"
     gene            complement(3651..8738)
                     /locus_tag="VFQ35_07410"
     CDS             complement(3651..8738)
                     /locus_tag="VFQ35_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930496.1"
                     /translation="MEPKPSADAKVFVDAKPPRAPLEPRATPNSKERLALGSALPLDA
                     TLDDATAPRSPLPSSPPPKSRQQVARELIAALRQELDQHPKPLRAGRLWFEAARLLES
                     PLGELKEAADAYLRAHGVLAEHLPSIRGARRVLLTLGRAAEALPLFDAELKLTSEADR
                     KAQILYDKAAVLEDVLGQRKEAREALELAAELAKSDATRVKSTERAAALNRAWDSLGR
                     AFEREANAVKDDARHRSALLAARARLLEAHRGDGATAVELYQQALAGEPRFYSALHAL
                     KRLHYGHQRWRDLIQVLEREANVAHDPDSRGMAYYRMGRVWLDRLGSVDDAIAALERA
                     REHLPSDRMILEELARLYEAASQPDKLCQILEQMAEREESRADQLACYLRIAELRQNE
                     LEDESKAIVWYERARGVDRAYLPAIQALSELYTRRREFENLIEVHAGEAEAAKDPARR
                     AGAHARIAEILERRLGRPDEAMQQHARALGLVPGYAPSFKALARLLTQTQRYAELIEL
                     YERAVDQASETESKVTYLFKIGRLYEDALGSPAQAVVAYRRILEFAPHELGALHALQR
                     AAERAGAFKDLIAALELEAEKTLERRRKLELYLRAGEVAELELGDDALAIASYRRLVD
                     IDRSYAPAYTALGRLYHKAGRYEDLIETYKSELRLLPKGAALSALLFRMGQIYEQKLG
                     RDDDALGAYRRAVEADPTHRAAVRALERRLEHKGAWEELVRVLESELSALDEASQRAR
                     ALLRIGEVNENRLRAPERALFAYEQALALDPGLTSARDGRVRLLTDARDYRKLVDELE
                     REAAQQRDDRLAIAALLRAGEVYRDELAEPIRAIRAFETVLSRDPGHIEALLALETLY
                     AERGAFEALANVYATEARILSEGATRVAALRELGRLQQSGRVENSDRGRQAFEAILQI
                     MPEDVGALFALERLALSQSDGELLAHIDAKLSRILPDPLSIAQYETRLGEVLEAQEDP
                     QALELYRSALVHDPESIGATRGLSRIAERRADPILLGEAAEREARVSLDVSRAASELV
                     QAAELFIVGSDTERAVQALSRALEIDPDHELSAARLSELLAERGDIETLIALLTRAAG
                     SAKRPSRMADLWGAVADLHADARDDLPAALAALQRAHGISPRQGSIVLKLADLQFRQG
                     HWAEAADRLKQALDDRPTPDLAIEAHLRLALIYDEHLGEPERARAHLDSVLNMEPNHG
                     AALQRLVQLQIRNGKLDQAADTAARLVRVSPARDARITALTLLGRVERTRNQLEAAAH
                     AYEQVVALEGVGGEAAHELRELVSSNSGDGPPFTRYVSALSRYIEETTAPRADAFLEI
                     ARALSDRLGQPEAALGWLERGLRAHPQDIALHDELGQALLAAGQHQRAYSEFERVVQN
                     DVGRESAWRRLSEALNGLQRNQDPAYGMSPLVALGFANDLERASVASRAARPAAALPG
                     GLGRSELLAIAPRNSDDPAARLIEALGDIAGKVFPPDLERFGVSARDRLSSKSNHPLR
                     ALTERIASIFGLADYELYLYRGQQGGGIEIELTDPVSILVPAQIAGLSESEQTFALAR
                     VLATASLGLSPIDRLPVASISALLAAAARLVEPNYGAGQFDEESLASLARRVSRSLPW
                     LGRGPIEDAARAYTAATRLETSEWVLAVKLTAGRAAILLADELPKSLALVRKYESEPF
                     ATRLTHDLLRFWVSETCLTLRRRLGLG"
     gene            9335..>10137
                     /locus_tag="VFQ35_07415"
     CDS             9335..>10137
                     /locus_tag="VFQ35_07415"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9930497.1"
                     /translation="MPAELLVFTSVRALDPSAGLDAVIDVVVENGRLTRVGPQAAAAL
                     SRAETARVIDGRGLWLMPAFVDLHAHLREPGQEYKEDIASGLRAAAAGGYAHVLVMPN
                     TRPVNDTRAVTEMMLRRASECDGPSLHPIAAITMGQKGKELTEMAELREAGAVAVSDD
                     GVCVMSSAVMRRALEYAKNFDMPVIQHAEDHDLTQGADMNEGAVSARLGLRGWPRAAE
                     DVIVARDVILAEYVKARYHVAHVSTLGAVRILREAKGRGIDVTCEVTPH"
BASE COUNT         1699 a         3274 c         3453 g         1711 t
ORIGIN      
        1 accgcccaac tcaggccttg gcgacgcagc gcggagagca gggcccaatt gaagggcttg
       61 cccgccgaag gcatctcgac cgaccagggc acggacgcga agggagtctg aatgtcccag
      121 agctcgagtt cggcgcgcaa atacgcgtac tggattggtc gcgcgctgcg atacaagcca
      181 gcccagaaag cggcaccttg ctgggtgaac gcgagccgca cccgcgaggg atgactcgga
      241 tcgatttcga cgacgcgggc ctgtagctgt tcggagaagg cgatctcgca atccacgcac
      301 agggtcggcg ccggcccccg ccgctcggtg ggctggcgat ggtcaccaga cccgagcgtg
      361 atggcccaga agcttccgtc cggggcctgc ccggtcaggc gcagctccag cgtatcgttc
      421 gggcggagtg cggccggtaa ggtggcagag tcgttgaaaa ccaggatgtc cggcgcgcgc
      481 aggatgcggg gcagctcact caactcacga tcagcatagg cgttcgattc tgggtcgaac
      541 agcaacaagc gagtgagatc gctcggacgg cgcggccgcg ccgcagccgc gatcatggat
      601 gcctccagtt ctcgacggtg acgcgttcgc cagattgcca agcaccgcgt tccaggtagt
      661 cgaggatggc actcgcgact tcggtcggct tggcgagctc ggcggtcggc gcctcgggta
      721 gtgcgtcgcg gtgcatgcga gtatccattt ctccgggatc gatgctcagg acatcgacgc
      781 cgaattccga tagttcctgc gcccaaaggc gagataggtg gtcgagcgcg gctttggaga
      841 cggagtatgc cccccagcgc tcgtacggtg tcacggccgc gtcggatgag atgtgtacaa
      901 tcaagccctc gccttgaatg gccatgttgc ccgcaagggc cttgctcagt cgaaagggcg
      961 cgagcagatt gagctccagc acgtcggcga gatcctcgca ctcggtgtcg agcaacaggc
     1021 gcagcggcag tggccccaag gtgctggcgt tatgaaagag cacctcgatg ggcccgacca
     1081 aagcggcggc ggcaccggtc aaagggtaga tttcattctt gttagagaga tccgcagcca
     1141 gggcgtgcgc ttcaccacct cgttcccgga tctgttggac gacgatgtcg agctccggcg
     1201 ctccgcgagc cgtcaaaacg acacgcgcgc cccgagccgc gagttcggcg ccaagcgccg
     1261 cccccaagcc ccggctgccc cccgtcacca gcgcgccgcg tcccgcccaa tagcctcgtt
     1321 gtgcttgctg attgctgtga tccatggcgg tcaagctagc cgctctggtc agtaagatgg
     1381 cattgctgcc agtttactgg cagaattcca gcgcgatgtc agacgagatg gcgcagcgct
     1441 tgggacaaaa catcgttcag ctccggcaat cccgcggctt tacccagctg cagatggcca
     1501 agctcgccaa cctaccccga gccacttggg cgctgttgga gtcgggtacg gcgaacccca
     1561 cgttgacggt gttgcaggcg gccgcggccg cgctgcaggt caacttggac gagctcttgg
     1621 ccgagcccaa agcgctggtt caacactatc cgcgcggtag cttgccgtca cgccggcgcg
     1681 gctcggtcac ggtgagttcg ctactgcccg acaacatccc gggcacgcaa atcgaacgta
     1741 tcgaactgcc tcccggggct cgcttgacgg gggtaccaca cacgttcggt tcgcgtgaat
     1801 acctgagttg cgagagcggc gagatcgagc tcgtcgccgg cggcacgcgc ttcaccttga
     1861 agccgggaga cgtggtggtg ttccgtggcg atcaacgcca ctcctacgcc aatctcgagc
     1921 gtagccaagc gatcgggttt tcggtcgtcc tcgtcaatcg caaggtggat tgacgaggac
     1981 gcacccgacg ccgagcccgg gaattcatcc atccagagcg gtttcagcgc gcgatggagc
     2041 tgcccgcgtt ggcgagctca gcgtaggcgg gtgccggcac ggattcaccc gcgcgcgtgg
     2101 atgttccgac tttccggctg tcggggcgca gcgcccagga cgcgaaaacg acgcccaaga
     2161 gcacgatggg tgtcagaatg tttcctgcgc tgtcccccac tgccgcgtgc gacactgcgg
     2221 ctcccgtcag gtcgaatacc ataccggcat aggcccactc tttgagccgc ggcagccccg
     2281 gcgcgagcac ggctgccacc ccgagcacct tccaagctcc gagaagggtc atgagataag
     2341 cgggatagcc gaggtgcgcc atttgctcga ccatctgcgg tgggcgcagc agatccatga
     2401 ttccgccgaa accaaacgcc agcgcaacta aagctgttgc cgcccaataa ccaatgatcg
     2461 ccttcttcat gtgagtgtcc cctgaatggt aataattgtg tttggaggtc gcccagcacg
     2521 ggtgtttccg agtatccttt ggatctcgct gcgcccgttc gaactttgaa cgactgtaat
     2581 gtcagacgaa tcggtgcgca ccgcaatgca gcaaatggtg gactcatttg ctacaaaatg
     2641 atcccaatga aggacgcccc cgaaacttcg gagctgatgg cattcgccaa aacggtagaa
     2701 accaagtcgc tgagccgcgc cgccgccgag ctgggcgtcc cgagggccac gatcagccga
     2761 cgcctggcgc gcttggagga gctgttgggc gtccgcttgt tgcggaggag cacgcgcagc
     2821 ctggctctga cggaggaagg taaggccttc tacgctcgta cccgagtggc gctgcaagcg
     2881 gtggccgagg cgcaggcgag cgtacaaccc atggccgacc ggctgcgggg cgatttgagg
     2941 gtctcttttc cgcccattca agacgcggcg atgtttggtt tgctcgtgga cttcgcgcgg
     3001 cgcaatccga gcgtacgctt gcacgcgcat ttctcgagtg tgcacgtgga tttgcgacgc
     3061 ggtgactatg acgtggcgct gcgcggtatc atggagagtt cacctgggct ggtccaacgc
     3121 acgctgctgc gggtaccgct catcgcggtc gcttctcccg agtatttggc cgagcacggc
     3181 gtaccgcgca gaaagcagga tttgcggcaa catcggtgtc tgctcggctt cgcgcgtgga
     3241 gagatcccgg agtcgtactg gcccgtgctg ggaggcggca agctgcgtgt ggagggttgt
     3301 ttcttctcga atgacctctc cctcctgtgc gaagcggcgc ggcagggtct cgggatcgcg
     3361 ctgttgcccc ggatgttcgt ggatccgctg ttgagcatgg gcctgctcgt tcacgtgctc
     3421 ccgagcgtga ttggcgcaga gtcgaggatg gcgctcgtct atccagagcg cgagttcatg
     3481 gctccgcagg tgcgcgcctt catcgacgag attgcgcgac ggatccccga ggagctggcc
     3541 aacggctcac tgctcccgca gcagccgcgc cctccgtccc ggctgggccg gaagcgatag
     3601 ccaagccacg gcaatgtcgg ggtgcgcgaa gcggcggggc gcccgtcgaa ctatccgagg
     3661 cccaagcggc gccgtagggt gaggcaggtc tcgctgaccc aaaaccgcag taagtcgtgg
     3721 gtgagccgcg tggcgaacgg ctccgattcg tacttgcgca cgagagcgag gctcttcggc
     3781 agctcgtcgg ctagcaagat cgcggcgcgc cccgccgtga gcttgacggc cagcacccac
     3841 tccgaagtct cgaggcgagt cgccgcggtg taggcccggg ccgcgtcctc gattgggcca
     3901 cgaccgagcc acggcaaaga gcgagaaacc cgacgcgcca agctcgccaa ggattcctcg
     3961 tcgaattggc cggcaccgta gttgggctcg accagccgcg ccgccgccgc gagcaaagcc
     4021 gaaatggagg ccaccggcag cctgtctatc gggctcaggc cgagcgaggc cgtggcgagc
     4081 actcgggcca gagcaaaggt ttgctctgac tcggacaggc ccgcaatttg cgccggcacc
     4141 aggatgctca ctggatcggt gagctcgatc tctatgcctc cgccctgctg gccgcgataa
     4201 aggtacaact cgtagtcagc cagcccgaaa attgaggcga tccgctcggt gagcgcgcgc
     4261 aaaggatggt tggatttcga gctcaggcga tcgcgagccg agacgccgaa acgttcgaga
     4321 tcgggaggga agaccttgcc tgcgatgtca cccaacgctt cgatcaagcg cgcggctgga
     4381 tcgtccgaat tgcggggcgc aatcgccaag agctcgctcc ggccgaggcc accgggcaaa
     4441 gccgcggccg ggcgagccgc gcgggaggcg acgctggcgc gttcgagatc gttggcaaag
     4501 ccgagcgcta ccagcgggga catgccgtac gcgggatctt ggtttcgctg taagccattc
     4561 aaggcctcgc tcaggcgacg ccaagcgctt tcccggccca cgtcgttttg aacgacgcgc
     4621 tcgaactccg agtaggcgcg ctgatgctgg ccggccgcga gcagcgcctg cccgagctcg
     4681 tcgtgcagcg cgatgtcctg cggatgagcg cgtaaaccgc gttcgagcca gcccagggcg
     4741 gcttcgggct gtcccaggcg gtccgacagg gcgcgcgcga tttcgaggaa cgcatcggcc
     4801 cggggagccg tggtctcttc gatgtagcgt gagagcgccg agacgtagcg cgtgaacggc
     4861 gggccatcac ccgagttcga gctgacgagc tcacgcagct cgtgggcggc ctctcccccg
     4921 acaccttcga gcgcgacgac ctgctcgtag gcatgcgcgg ccgcctcgag ctggttgcgc
     4981 gtgcgctcca cacgaccaag caacgtgagc gcggtgatgc gcgcgtctcg ggccgggctg
     5041 acccgcacca gacgcgccgc ggtgtccgcg gcctggtcca gcttgccgtt gcgaatttga
     5101 agttgaacca gacgctgcag cgccgcgccg tggttgggct ccatgttgag cacggagtcc
     5161 aggtgggcgc gggcccgttc gggctcaccc aagtgctcgt cgtaaatcaa cgccaagcgc
     5221 aggtgcgcct cgatcgcgag gtcgggggta ggtcggtcgt cgagcgcctg cttgaggcga
     5281 tcggcagcct cggcccaatg cccttggcga aactgtaggt cggcgagctt gaggacgatc
     5341 gagccctgac gcggcgaaat gccgtgcgcg cgttggagcg cggcgagcgc cgcgggtaag
     5401 tcgtctcgcg cgtcagcatg cagatccgcg accgcgcccc agagatcggc catacgcgac
     5461 ggccgcttcg cggagcccgc ggcgcgcgtc agaagcgcaa tcaaggtctc gatatcgcca
     5521 cgctccgcga gcagctccga cagccgcgcc gcagagagct cgtgatcggg gtcgatctcg
     5581 agcgcgcgtg acaacgcctg caccgcgcgc tcggtatcgg agccgacgat gaacagctcg
     5641 gccgcctgca cgagctcgct cgcggcgcgc gacacgtcca gcgagacgcg cgcctcgcgc
     5701 tctgcagcct ctcccagcag gatcggatcc gcgcgccgct cggcgatgcg gctcaagccg
     5761 cgcgtcgcac cgatcgactc gggatcgtgg accagcgccg accgatagag ctcgagggcc
     5821 tggggatctt cttgcgcctc gagcacctcg ccgaggcgcg tctcgtattg ggcgatgctc
     5881 agcggatccg gcaggatgcg agagagcttg gcgtcgatgt gcgcgagcag ctcgccatcg
     5941 ctttgcgaca aagccaagcg ctcgagcgcg aacaatgcgc cgacgtcctc cggcatgatc
     6001 tgcaagatgg cctcgaaggc ctgacgtccg cggtcggagt tttccacgcg cccgctctgt
     6061 tgcaaccgcc cgagctcgcg cagggcggca acgcgcgtcg caccctccga gagaatgcgc
     6121 gcttcggtgg cgtagacatt ggcgagcgcc tcgaacgccc ctcgctcggc gtagagggtc
     6181 tcgagcgcga gcagcgcttc gatgtgcccc ggatctcgcg atagcaccgt ctcgaaggcg
     6241 cgaatggcgc ggatcggctc cgccagctcg tcgcggtaca cttcaccggc gcgcagcagg
     6301 gccgcgatcg ccaagcgatc gtcgcgctgt tgcgcggctt ctcgctcgag ctcatcgacg
     6361 agcttgcgat aatcgcgcgc gtcggtgagc aagcgcaccc gaccgtcccg cgcggaggtc
     6421 aggcccggat cgagcgcgag ggcttgctcg tacgcgaaca gagccctttc cggtgcgcgc
     6481 agccggttct cgttcacttc accgattcgg agcagcgcgc gtgcccgctg gctcgcctcg
     6541 tctagcgcgg aaagctcgga ctcgagcacg cgcacgagct cctcccatgc gcccttgtgc
     6601 tcgagccggc gctccagggc ccgaactgcg gcgcgatggg tcgggtcggc ctcgaccgca
     6661 cggcggtagg cgccgagcgc gtcgtcgtct cgccccagct tttgctcgta gatttggccc
     6721 atccggaaca gcaacgcgga cagcgccgcg cccttgggca acaagcgcag ctcgctcttg
     6781 tacgtctcga tcaggtcttc gtatcgaccc gccttgtgat agagccgccc gagcgcggta
     6841 taggcgggcg cgtaagagcg atcgatgtcg accagccggc gataagacgc gatagccaga
     6901 gcgtcgtccc cgagttcgag ctcggcgacc tcgcccgctc gcaggtagag ctcgagcttg
     6961 cgccgccgtt cgagcgtctt ttcggcttcg agctcgagcg cggcgattag atctttgaac
     7021 gcccccgcgc gctcggcggc gcgttgcagc gcgtgcaagg ccccgagctc gtgcggggca
     7081 aactccagaa tccggcgata cgctacgacg gcctgagccg gtgagcccag ggcgtcctcg
     7141 tacagacgac ctatcttgaa gaggtaggtg accttactct cggtctcgct cgcttgatcg
     7201 acggcgcgct cgtaaagctc gatcaactcg gcgtagcgct gggtttgcgt gagcaggcga
     7261 gcgagcgcct tgaaggacgg cgcatagccg gggaccaagc cgagcgcacg ggcgtgctgc
     7321 tgcatggcct cgtcggggcg gcccaagcgg cgctcgagaa tctctgcgat gcgcgcatgc
     7381 gcgccagcgc gtcgggcggg atctttcgcg gcttcggctt cgccagcgtg aacctcgatc
     7441 agattctcga actcgcgccg ccgcgtgtac agctccgaaa gagcctggat cgcgggcaag
     7501 taagcgcgat ccacaccgcg cgcccgctcg taccatacga tcgccttgct ctcgtcctcg
     7561 agctcgtttt gacgcagctc cgcgatacgc aggtagcacg cgagctgatc ggcccgggac
     7621 tcctcgcgct ccgccatctg ctccaggatt tggcacagct tgtcgggctg actcgcggcc
     7681 tcgtacagcc gcgcgagctc ctccaggatc atgcgatcgc tgggcaggtg ttcgcgagcg
     7741 cgctccagcg cggcgatggc atcgtctacg ctgcccaacc ggtcgagcca cacccggccc
     7801 atccggtaat aggccatgcc gcggctgtcc ggatcatgcg cgacgttggc ttcgcgctcc
     7861 agcacttgaa tcaagtcgcg ccaacgttgg tgaccatagt gcaggcgctt gagcgcgtgc
     7921 agcgccgagt aaaagcgcgg ttccccggcc aaagcctgct gatagagttc gaccgcggtc
     7981 gcgccgtcgc cccggtgggc ttccagcagc cgcgcgcgag cggccagcag cgccgaacgg
     8041 tggcgcgcgt cgtctttgac cgcgttcgcc tcgcgctcga aagcgcgtcc gagcgagtcc
     8101 cacgcacgat tcaacgccgc cgcgcgctcg gtgctcttca cgcgcgtggc atcgctcttg
     8161 gcgagctctg ccgcgagttc gagcgcttcg cgcgcttcct tgcgttgccc gagtacgtcc
     8221 tcgagcacgg ctgccttgtc gtacagaatt tgagcttttc tgtccgcttc ggaggtgagc
     8281 ttcagctccg cgtcgaacag cggcagcgct tccgcggcgc gccccagcgt cagcagcacg
     8341 cggcgcgcgc cgcggatcga gggcaagtgc tcggccaaca ctccgtgcgc acgaagatag
     8401 gcgtccgctg cttctttcag ctcgccgagc ggagactcca agagccgagc cgcctcgaac
     8461 cacagccggc ctgcgcgcag cggcttggga tgctgatcta gctcctgccg tagcgccgcg
     8521 atcagttcgc gtgcgacctg ctgacgcgac ttcgggggtg gtgagctggg cagcggcgag
     8581 cgcggcgcag tggcatcgtc caaggtcgcg tccaatggca gcgcggatcc caaagccagg
     8641 cgctccttgg aattgggcgt cgctcgcggc tcgagagggg cacgcggtgg ctttgcgtcc
     8701 acgaaaacct tggcgtcggc agacggcttg ggttccacgg gcggcaaggg gcgggaggct
     8761 gcggcagaat tgcggtaggc gggtgcggcg ctaggcgacg gcgggcctgc ggcagcaggg
     8821 ttactgcctt gcggcgggcg gggcggaatg ggcggacgca cgatgagttt tccgggggca
     8881 ggtcgttggg ccgtaaccgg ttcgtcgggc tcgagctccg gcaagtcatc ggggggaggt
     8941 ggctcagatc cggatcctcc ggacttccgg ggcggaaacg gcggcggccg caaactaccg
     9001 ggcttggagg ggacagccgg ggccgggccg cgcgagcgcg tggcgggcgg gctcgcgctt
     9061 gcagcaggat tcgagccggt ttcgacggcg gggctcggag gcgtggagtt tcccgccccg
     9121 gcgctgctct ggcgaggcgg aggaatcgta gcgcgggtac cggacggccg cggagggctc
     9181 ggcttattcg gactcttgaa gtcgtcagcc atcttgatag tggggcgtgc ccgcgtcccg
     9241 ggctgcaggc ccagcgtaac accgctcacc cgagtcagct cgcaattcgc ctacccctgc
     9301 accaaccagt ttccgcatgc tatcacccgc cgccgtgcct gctgagttgc tcgtcttcac
     9361 gtccgttcgc gccctcgatc ccagcgcggg tttggatgcc gtcatcgatg tggtggtcga
     9421 aaacggtcgt ctcacgcgcg taggcccaca agccgccgct gcgttgtcgc gtgctgagac
     9481 ggcgcgagtc atcgatggcc gcggactttg gcttatgccg gcgttcgtgg atttgcacgc
     9541 gcacctccgt gaaccggggc aggaatacaa ggaagacatc gcgagcgggt tgcgagccgc
     9601 cgcggcgggt ggctacgcgc atgtgctggt gatgcccaat acgcggcccg taaacgacac
     9661 gcgagcagtc accgagatga tgctgcgtcg cgcttcggag tgcgatggcc caagccttca
     9721 cccgattgca gccatcacca tggggcagaa gggcaaagag ctcaccgaga tggcagagct
     9781 gcgcgaggcc ggagctgtgg ccgtcagcga cgacggagtt tgcgtgatgt cgagcgcggt
     9841 catgaggcgc gcgctcgaat acgcgaagaa cttcgacatg cccgtaatcc agcacgccga
     9901 ggatcacgac ctcacgcaag gcgcggatat gaacgagggc gcggtgtcgg cgcggctggg
     9961 gctacggggc tggccgagag cggccgagga cgtcatcgtc gcgcgcgacg tgatcttggc
    10021 ggagtacgtc aaggctcgtt atcacgtagc ccacgtttct acgttgggag cggtgcgcat
    10081 cctgcgggaa gccaaagggc ggggcatcga cgtgacgtgt gaggtgactc cgcatca
//
LOCUS       DASTKS010000359         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_950159, whole genome shotgun sequence.
ACCESSION   DASTKS010000359 DASTKS010000000
VERSION     DASTKS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950159"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFQ35_07420"
     CDS             complement(<1..257)
                     /locus_tag="VFQ35_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9930498.1"
                     /translation="MQHAAPSAKNLAAWCAEYCALGMADVADRIRTAGGHATMLDVGC
                     GMGNGLRIFDRLGFDVTGIDMSAPEVELARSLGFDVLCESA"
     gene            962..1630
                     /locus_tag="VFQ35_07425"
     CDS             962..1630
                     /locus_tag="VFQ35_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9930499.1"
                     /translation="MDLKTGFYAQVFENNRKWAAEKLALDPKYFERLAEGQSPEILYI
                     GCSDSRITAEELMGAGPGEVFVHRNIANVVGASDLNALSVIEFAVAHLNVKHVVVCGH
                     YECGGVRAAMQPKDLGVLNPWLRNIRDVYRLHVEELSAIQDEHQRYRRLIELNVEEQC
                     VNVIKTAVFQKMFLATGRPTVHGWVFDIRGGRLVDLRIDFARKLTGIREVYDLGHAIP
                     ERVG"
     gene            complement(1643..2524)
                     /locus_tag="VFQ35_07430"
     CDS             complement(1643..2524)
                     /locus_tag="VFQ35_07430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9930500.1"
                     /translation="MRTGKLASWLGYFSAFWSGAERYYEYLGDDVIHGQSEGFSDPKK
                     PLWLNLGYWTQARTYPEAARALACQLADAAELGPSDVQLDVGFGFAEQDFLWLEEYGV
                     ARITGINITEMQVERARARARERNLEGRLELMVGSATEMPFEAGSFTKVTALECAHHF
                     ATRERFFAEAFRVLKPGGRLALADGVPLPGHGAPSLMTRVILRQWASPVENFYDRNVY
                     AQKLRACGFENVRIRSIREQVYPGAVKYSNLRRSGVKMQDALVELTPAEIERNLKLWE
                     PLRITDYLIATADKPAG"
BASE COUNT          503 a          867 c          885 g          445 t
ORIGIN      
        1 tcggcggact cgcacaggac gtcgaagccg agcgaacggg cgagctcgac ctcgggcgcg
       61 ctcatgtcga tgcctgtcac gtcgaagccg agacggtcga aaatgcgcaa gccgttgccc
      121 atgccgcatc cgacgtcgag catggtggcg tgcccgccgg ccgttctgat gcgatccgcg
      181 acgtcggcca tccccaacgc gcagtactcg gcgcaccagg cggccaagtt cttcgcgctc
      241 ggagccgcgt gctgcatcgt ttgcataagc tcaacgtatc caggccccga gtacagctcg
      301 gcgagctcat cgtcgctgta gccgcgcgcc gtgaactgca ggccacaggc ggcgcagcgc
      361 ataatctcga ggtcgaatcg tgatgggtcg gcgacgttgc gtgcgtaccc tcgtaggtcg
      421 ccgaagtcgg agcggagacc gagcgacgaa agatttggcg aagcgcagag cgggcaagag
      481 gcgtgtgggg tcggaagcac ggctcatggt accaggccaa gcgctcaaac cgagcaaagc
      541 cctcaagcgc aaaaaccgca ttcgagtcgt ctgttcatgc cttgcgccgc cacgagctga
      601 gccggcaata tcgctacact ggggtggacc tacggcgtaa cacagcgtcg ggcacgctac
      661 ccagcggaga cgctccatgc tgaaggtcga cacgcgggac cttcggcgtc tcgaggaggc
      721 acctcacgcg atacgccaag cgctccctgc cgtacgctat caggaacggg ctgaacacga
      781 cggcgttgtg cgacctccgc gcgcgcgaac ctggccctgc gccgagagcg cggaatccga
      841 gtgcgggccg cctgacagag gccgcttgtt gatgtaatcg aagctgggca cgtgtgcccc
      901 ctactctaga ctggcgcggc gcccagcccg gggagcgcgc tcgaaccaag gagacacagc
      961 cgtggacttg aagacgggat tttatgccca ggtgttcgag aacaaccgca agtgggcggc
     1021 ggagaagctc gcgctcgatc caaagtactt cgagcgtctc gcggagggcc agagcccgga
     1081 gatcttgtac atcggctgct ccgatagccg aatcaccgcc gaggaattga tgggcgccgg
     1141 gccgggcgag gtgttcgtgc accggaacat cgccaacgtg gtcggcgcga gcgatctcaa
     1201 cgcgctcagc gtcatcgagt tcgcggtcgc acacctcaac gtgaagcatg tggtggtgtg
     1261 cggtcactac gagtgcggcg gcgtccgggc cgccatgcag cccaaggatt tgggcgtgct
     1321 caacccgtgg ctgcgcaaca tccgcgacgt gtaccgccta cacgtggaag agctgtcggc
     1381 gattcaagac gagcaccagc gctatcggcg cctgatcgag ctgaatgtcg aggagcagtg
     1441 cgtgaacgtg atcaagaccg cggtgttcca gaagatgttc ctggcgacgg gacgtccgac
     1501 cgtgcacggc tgggttttcg acattcgcgg cggccggctg gtcgacctgc gcatcgactt
     1561 cgcgcgcaag ctgaccggga tccgcgaggt ctacgatctc gggcacgcga ttcccgagcg
     1621 cgtcggctga gattgcgccc ggtcagcccg ccggcttgtc ggccgtcgcg atgaggtagt
     1681 cggtgatgcg cagcggctcc cagagcttga ggttgcgctc gatctcggcc ggcgtgagct
     1741 cgacgagcgc gtcctgcatc ttcacgccgc tacgccgcag gttggagtat ttcaccgcgc
     1801 ccgggtaaac ctgctcgcga atggagcgga tgcggacgtt ctcgaacccg cacgcgcgca
     1861 gcttctgtgc gtacacgttt cgatcgtaga agttttcgac gggcgaggcc cactggcgca
     1921 ggatcacgcg cgtcatcagg ctcggggcgc cgtggccagg cagcgggacg ccgtcggcga
     1981 gcgcgagacg cccgccgggc ttgagcacgc ggaaggcctc tgcaaagaag cgctcgcgcg
     2041 tcgcaaagtg atgcgcgcac tcgagcgccg tgaccttcgt gaacgagccc gcctcgaacg
     2101 gcatctccgt cgctgagccg accatcagct cgagccggcc ctcgaggttc cgttctcggg
     2161 cccgcgctcg cgcgcgctcg acttgcatct cggtgatgtt gatgcccgtg attcgggcga
     2221 ccccgtactc ctcgagccaa aggaagtctt gctcggcaaa gccaaagccc acgtcgagct
     2281 ggacgtcgct cggcccgagc tccgcggcgt ctgcgagctg gcaggcgagc gcgcgggccg
     2341 cctctggata agttctggcc tgggtccagt aacccaagtt gagccagagc ggcttcttgg
     2401 ggtcactgaa gccctcagac tgaccatgga tgacgtcgtc accgaggtac tcgtagtagc
     2461 gctcagcccc tgaccaaaac gccgaaaaat agcccaacca gctcgcaagc ttcccagttc
     2521 gcatcgcact ctctccgccg gacggaccac gtagtccgaa gcgcgcgcgc tctgcaactg
     2581 tggattcgag gcagacaaca aatcgagtcc tagagataga gagctcagtc gaccttccaa
     2641 tacttgcgcc agaacgcaca ctcttccgcg cggaagttct cgagcacttg ccagtcgggc
//
LOCUS       DASTKS010000360         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1173810, whole genome shotgun sequence.
ACCESSION   DASTKS010000360 DASTKS010000000
VERSION     DASTKS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173810"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFQ35_07435"
     CDS             complement(<1..797)
                     /locus_tag="VFQ35_07435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET9930501.1"
                     /translation="MKAQVRELLGPSGPLARTLSGYEERPGQLAMAEAVERALRDERV
                     VLCEAGTGTGKTLAYLLPALLSGKKVVVSTATRALQEQIFFKDIPLVARAFGLEPRVA
                     LMKGVSNYLCRRRFQEFRRSADSVRPGFSRSLAVVESWVNETHTGDLGELAALAEDDA
                     VRLEVGSSSDTRIGPSCQYYEDCFVTQMKREAAAAQLVVVNHHLFFADIALRGPHPAR
                     VLPDYDAVVFDEAHQLEDVATEFFSVRISSGGDMVRALPTVAWTWRCR"
     gene            complement(794..1165)
                     /locus_tag="VFQ35_07440"
     CDS             complement(794..1165)
                     /locus_tag="VFQ35_07440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9930502.1"
                     /translation="MVQKSVVKNRPPSVSSTPPSCPQRRIAGGSRREATERVSLKAPG
                     FETTGWTLNISRGGLRAILEDRLTMGVEYEIVVGDAATPRRATLVWAQEQSDGQIVGL
                     KYLDIEASMPPPDGSTPPLEE"
     gene            1409..1840
                     /locus_tag="VFQ35_07445"
     CDS             1409..1840
                     /locus_tag="VFQ35_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930503.1"
                     /translation="MPKLSPTELKKALQAQGFEIYRTTPTQVMLADRVRDNLLMDSAV
                     AVGVADEFTVSVTVRAQSSDFPGEGESALYGRARRCAEAMQQRGFRESGTQMVPVHDP
                     GDRSRILDTWYEVRFERKAADLEHVVAELKAALALEKCARP"
     gene            1973..>2564
                     /locus_tag="VFQ35_07450"
     CDS             1973..>2564
                     /locus_tag="VFQ35_07450"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET9930504.1"
                     /translation="MLRFFRGGGVGQLVVGGVVSAIIVAFVVEFRAGRGPTAKLKREC
                     AVRIGSACVDPKDYFAEFGLIAPRGLEGPAAKRLGLRKKTLEGIVERELLVEKARALG
                     LGVGEDTIDSELEAGRAHVSLPAAEISKLSATLGLCRIDRGGQSCEPGSDRGVRQLRV
                     RKSPSDPFDYKLYEREIRMLTNRGPREFKEMQERELL"
BASE COUNT          454 a          827 c          830 g          453 t
ORIGIN      
        1 cgacagcgcc atgtccatgc caccgtcggc aatgctcgca ccatatcacc accgctcgaa
       61 atgcgaacgc tgaagaactc ggtcgcgacg tcctcgagct gatgagcctc gtcgaacacg
      121 accgcgtcgt agtcgggcag cacccgcgcc gggtgaggac cgcgcagcgc gatgtcggcg
      181 aagaacaagt ggtggttcac gacgacgagc tgcgcggcgg cggcctcacg cttcatctgc
      241 gtgacgaagc aatcctcgta gtactggcag ctcgggccaa tgcgcgtgtc gctcgacgag
      301 cccacttcga ggcgcacggc gtcgtcttcg gcgagggccg caagctctcc caaatcgccg
      361 gtgtgagtct cgttgaccca cgattcgacc accgcgagcg agcgcgaaaa gccgggccgc
      421 acgctgtccg cgctccggcg aaactcctga aagcgacgcc ggcaaaggta attgctgacg
      481 cccttcatca gcgcgacgcg cggctcgagg ccgaacgccc gcgccacgag cgggatgtcc
      541 ttgaagaaga tttgctcctg cagcgcgcgc gtcgcagtcg agacgacgac cttcttccca
      601 gagagcagcg ccggcagcag ataagccaga gttttgccgg ttccagtgcc ggcttcgcac
      661 agcacgacgc gctcgtcacg gagcgcgcgc tcgacggctt ctgccatcgc gagctgtccc
      721 ggccgttcct cgtaacccga aagagtgcga gcgagcggac cactcgggcc caagagctcg
      781 cgaacctgcg ctttcattct tcgagaggcg gggtcgagcc gtcgggcggc ggcatcgacg
      841 cctcgatatc gaggtacttg agcccgacga tctgcccgtc ggactgctct tgagcccaaa
      901 cgagcgttgc ccgtcgcggc gtcgcggcgt cgccgacgac gatctcgtac tcgacgccca
      961 tggtcaggcg gtcctccaaa atcgccctca agcccccgcg gctgatgttc agagtccagc
     1021 cggtcgtctc gaagccgggg gctttgagag acacgcgctc ggttgcttcc ctccgggacc
     1081 cacccgcgat gcgtcgctgc gggcagctag gcggcgtcga cgaaacggat gggggtcggt
     1141 tcttcacgac gctcttctgg accatagcac tctcctctat gtttcctgtc gcttcaccct
     1201 cggagagcca ttcctcatcg aaaattcaag ccgtagggaa agcttgacgc tattctggaa
     1261 gagacgctag cttcgcccgc tgaactccca gttcattcgg tttggggggc gttaccggct
     1321 acggcggagc tcatgcgcta tgacgcccaa gccagtcact cccgcgtgaa cgggccgcta
     1381 cttaggtttg ccagcccgac gctgtgaaat gccaaagctt tcgcccaccg aattgaagaa
     1441 agccctgcaa gcgcagggtt tcgagatcta tcgaacgacc cccacgcagg tcatgctcgc
     1501 cgatcgcgtg cgcgacaacc tgctgatgga cagcgccgtc gccgttggcg tcgccgacga
     1561 gttcaccgtt tcggtgaccg ttcgagcgca gtcgtccgat tttccaggcg agggcgaaag
     1621 cgcattgtac ggacgcgctc gacgctgcgc cgaagcgatg cagcagcgtg gttttcgcga
     1681 gagcggcacc cagatggtac cggttcacga cccgggtgac cgatcgcgca ttctcgacac
     1741 gtggtacgag gtgcgcttcg agcggaaagc cgcggacctc gagcacgtgg tcgccgagct
     1801 caaggcggct ttggcgctcg aaaaatgcgc gcggccctga ggtttggtta gcttccaccg
     1861 ccgttttcaa cggcgttcgt cagtggtctg ttcccacagc gcggaaaacg ggccgtggcg
     1921 tggatccgcc ttttggggcg tggtatgtgc ggcgcccccc ggttggtaaa agatgctcag
     1981 gtttttcaga ggcggcggag tcggacagtt ggtcgttggc ggggtcgtct cggcgatcat
     2041 cgtcgcgttc gtggttgagt ttcgagcggg tcgcggcccg actgccaagc tcaagcggga
     2101 gtgcgcggtt cggatcggaa gcgcctgcgt cgatcccaag gactacttcg ccgaattcgg
     2161 cctgatcgct ccgcgcgggc tggaagggcc ggccgcaaag cgcctcggcc tgcgaaaaaa
     2221 gaccctggaa ggaatcgtcg aacgcgagct cctcgtcgag aaggcgcgcg ctctcgggct
     2281 cggcgtgggc gaagacacga tcgactcgga gctcgaggcc ggccgtgctc acgtgtcact
     2341 gcccgcagcc gagatatcga agctcagcgc gactttgggt ctctgccgca tcgatcgcgg
     2401 cggccagagc tgcgagccgg gctcggatcg cggcgttcgc cagctccgcg tccgtaagtc
     2461 cccgagcgac ccgttcgact acaagctgta cgagcgagag atccgcatgc tcacgaaccg
     2521 cggcccgcgc gagttcaagg agatgcagga gcgtgagctc ttgg
//
LOCUS       DASTKS010000361         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_280574, whole genome shotgun sequence.
ACCESSION   DASTKS010000361 DASTKS010000000
VERSION     DASTKS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280574"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFQ35_07455"
     CDS             <1..232
                     /locus_tag="VFQ35_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HET9930505.1"
                     /translation="QFHVAGHTNYGTHIVDTHVGPVPQDVWEMLGRAHALGARASLLL
                     EWDSEIPDFETVHQDALRARAFLSTFHGVHAA"
     gene            229..1053
                     /locus_tag="VFQ35_07460"
     CDS             229..1053
                     /locus_tag="VFQ35_07460"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9930506.1"
                     /translation="MTQPNAEASLESLQSCFFAASTHPESVEAGLAEVASLAVEDLVR
                     AGPRMSARACLEVYRESYASRLVECLADDYPAVQFHLGAAQFEVLAASYVVAHPSRSP
                     SLNYFGREFAAFCRARGDVFVAELAELEWAMVEAIHASDAEALSLERLAQLPADEWAL
                     ARFTPNASLRLLEFTHPVNAFYQAYRDELPPPTPVDERTWMAVCRLGTSVWRFDLCSA
                     RFGLLEQLSRGVPLGEALERAGSRDGVSPDDVLAWFAKWIQDGFFTDIRPRVHPRP"
     gene            complement(1089..2867)
                     /locus_tag="VFQ35_07465"
     CDS             complement(1089..2867)
                     /locus_tag="VFQ35_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9930507.1"
                     /translation="MLLSLRRVYLLRSLLRFAPTEAQRLFALTMVIGAMSGLAAVAFH
                     LTIHLFEAHLIEKALDAPGRSWIVWTLVTPTLGGVVCGALLQYVVPNARGSGIPQVKV
                     AFSRGELVRARDALGKFFIGSLQIGSGASLGREGPTVQICAGIASALGRLAGVSAKSQ
                     RRLLPVGVAAGIAAAFNAPIAAVTFTIEEVVGHLDQAVLSGVIVAAAIAAVIERSVLG
                     EQAVFTVPDSYGLPHASSLLIYALIGIAAAVVSVLFTDSLLKVRAYFRSLQRVPLWVR
                     PGIGGALTGALAVLALLTMQARGVNGGGYATLSDALTGQLPTRIMLALCGLKLVATVF
                     SYSSGGAGGIFAPSLFMGGMLGGSIAILDQLVLGHGEHSAGAFALVGMGAVFAGVIRA
                     PITSVLIIIEMTRGYSLILPLMIANMTSYALARHVRPRAIYEALLEQDGVHLENVNHS
                     DGLEGRSIANAVNTTGPFHRFVLATAAPEISQAARSSSAQNVFPVLDNEDHLLGIVTP
                     EELRILETEPDLLPVVAAWDLMRAPVSVRPRDDVRTVLEVMLANRIREVPVTDDSGRF
                     IGFIDEVALANTLKRRSDPPPPGARR"
     gene            complement(3115..>3361)
                     /locus_tag="VFQ35_07470"
     CDS             complement(3115..>3361)
                     /locus_tag="VFQ35_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930508.1"
                     /translation="DIGMPEQDGYELIRQVRSLPRERGGDIPAAALTAWARPEDRQRM
                     LNAGYSLHLPKPVEPAELVAVVGTLSRFIRRRDGLPS"
BASE COUNT          584 a         1085 c         1111 g          581 t
ORIGIN      
        1 gcagtttcac gtcgcggggc acaccaacta cggcacgcac atcgtcgaca cccacgtggg
       61 gccagtgccg caagatgtct gggagatgct cgggagggcc cacgctctcg gtgctcgcgc
      121 gtcgcttctg ctcgaatggg actcggagat cccggatttc gagacggttc accaagatgc
      181 gcttcgggcc cgcgctttcc tgtcgacctt ccacggagtt cacgccgcat gacgcagccg
      241 aatgccgaag cttcgctcga aagcctacaa tcatgcttct tcgccgcgag cacacatccg
      301 gagagcgtgg aagcgggcct cgccgaagtc gcttcgctcg cggtcgagga tttggtgcga
      361 gcggggccgc gtatgagcgc tcgagcttgc ctcgaagtct accgggagag ctacgcctcg
      421 cggttggtcg agtgtttggc ggacgactac ccggcggttc agtttcattt gggggcggct
      481 caattcgagg tgctcgccgc aagctacgtg gtcgcgcacc cgtcgcggtc gcccagtttg
      541 aactacttcg gtcgcgagtt cgccgccttc tgtcgggcgc gcggtgacgt gttcgtggct
      601 gagctcgcgg agctcgagtg ggccatggtc gaggccattc acgcgagcga cgccgaagcg
      661 ctgagcctcg aacgcctagc tcagctgccg gcagacgaat gggcgctcgc gcgctttacc
      721 ccgaacgcgt cacttcggct gctcgaattc acccatccgg tgaacgcctt ttatcaagca
      781 taccgtgacg agctcccgcc gccgacaccg gtcgacgaac gcacctggat ggcggtttgc
      841 cgactcggta cgagcgtctg gcgtttcgat ctgtgctctg cccgcttcgg tttgttggaa
      901 cagctctcgc ggggcgttcc gcttggagaa gcactcgagc gcgccgggtc tcgggacggt
      961 gtttcgcccg acgatgtgct cgcctggttc gcgaaatgga ttcaggacgg cttctttacc
     1021 gacattcgcc ctcgcgttca cccgcgtccc tgagcgatcc accgagcttc cgctccgcaa
     1081 atgcgccatc agcgacgcgc cccggggggc ggcggatcgg accggcgttt caaggtgttg
     1141 gcgagggcca cctcgtcgat gaaaccgatg aagcgcccgg agtcgtcggt taccgggact
     1201 tcgcggatcc ggttcgcgag catcacctcg agcacggtgc ggacgtcgtc gcggggcctc
     1261 accgagaccg gcgcgcgcat caagtcccac gcagcgacca cggggagcag atcgggctcg
     1321 gtctcgagaa ttctgagctc ttccggcgtt acgatcccga gcaggtggtc ctcgttatcg
     1381 agcaccggga agacattttg cgcggagctc gagcgcgcgg cctggctaat ttccggcgct
     1441 gcggtcgcga gcacgaacct gtgaaaaggg cccgtggtgt tgacggcatt ggcgatgctg
     1501 cgcccttcga gcccgtcgga gtggttgacg ttctcgaggt ggacgccgtc ttgttcgagc
     1561 agggcctcgt aaatggcgcg cggccgcacg tgacgcgcga gcgcatacga cgtcatgttg
     1621 gcgatcatca acggcagaat cagcgagtag ccccgcgtca tctcgatgat gatcaacacc
     1681 gaggtgatcg gcgcgcggat cacccccgcg aacacggcgc ccatacccac gagggcgaac
     1741 gcgcccgccg aatgctcgcc gtggcccaac acgagctggt cgagtatggc gatgctgccc
     1801 cccagcatgc ctcccatgaa gagggagggc gcgaagatgc cgcccgcgcc accgctcgag
     1861 tacgaaaata cggtggcgac gagcttgagc ccgcaaaggg cgagcatgat tcgcgtgggc
     1921 agctgcccgg tgagcgcatc gctcagggtt gcgtagccgc cgccgtttac gccgcgcgcc
     1981 tgcatcgtga ggagggcgag gaccgcgagc gctcccgtca gcgctccgcc gatccccggg
     2041 cgaacccaca ggggtacgcg ctgcaagcta cggaagtagg cccgaacctt caggagcgag
     2101 tcggtgaaca ggacggacac gaccgccgcg gcgatgccga tgagcgcgta aatcaagagc
     2161 gatgaagcgt gcggtaggcc gtagctgtcc ggaaccgtga acacggcctg ctctccgagc
     2221 acgctgcgct cgatcacggc ggcgatggcc gctgcgacga tgacgccaga gaggacggcc
     2281 tgatccaagt ggccgacgac ctcctcgatc gtgaatgtca cggccgcgat cggggcgttg
     2341 aacgcagccg cgattcccgc tgcaacgccc accggcagga gccggcgctg actcttggcc
     2401 gaaacaccag ccaacctgcc gagcgcactc gcgattccgg cacagatctg cacggtgggg
     2461 ccttcgcgac cgagtgaggc gcccgagccg atctgaagcg agccgatgaa gaacttgccg
     2521 agcgcatcgc gtgcgcgcac gagctctccg cgcgagaacg ctaccttcac ctgggggatg
     2581 ccgctgcctc gtgcgttcgg cacgacgtac tgcagcaggg ccccgcagac caccccgccg
     2641 agcgtcggcg tcacgagtgt ccagacaatc cacgagcggc cgggggcgtc gagtgctttc
     2701 tcgatgagat gagcttcgaa gaggtgaatc gtcaggtgaa acgctactgc ggcgagcccg
     2761 ctcattgcgc caatcaccat cgtcagcgcg aagagccgct gcgcttcggt cggtgcgaag
     2821 cgcagcaacg agcgcaggag atacactcgc cgcaagctga gcagcaccgc gaggcgtcga
     2881 cgcaggccgc tccttcgaga gcgcgggccg ttcagcgatg tcgggagagg cgtacgtatt
     2941 ggctctggcg tcattcgtcc cggactcccg gatcctgtaa cgtatcagcg cctcgtgtcg
     3001 acctgggcgc gctgcaagat tcttttggag ggtatggaac tcgcgccttt ccggcacgtc
     3061 gtagcttccc gaaaagtatc gtgcggcgac gttgcaagtt cgtttcgtcc gaacctagct
     3121 cggcaatccg tctcggcggc gaatgaagcg gctcagagtt ccgacgacag cgacgagctc
     3181 cgccggttcg accggcttcg gtaggtgtaa ggagtagccg gcgttcaaca tgcgttggcg
     3241 atcctccggt cgtgcccagg cagtgagggc tgcggccggg atgtcgccgc cgcgctcacg
     3301 cggcaacgag cgcacctgtc gaatcaactc gtacccatct tgctccggca taccaatgtc
     3361 c
//
LOCUS       DASTKS010000362         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_727145, whole genome shotgun sequence.
ACCESSION   DASTKS010000362 DASTKS010000000
VERSION     DASTKS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727145"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFQ35_07475"
     CDS             <1..225
                     /locus_tag="VFQ35_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930509.1"
                     /translation="RLRNVKARRDAELVPNDPERLHPPIHAAGPLPTSAEKAPRPEIA
                     GSPEAPRAPAPRAKTAAEILLERRRSRSER"
     gene            259..903
                     /locus_tag="VFQ35_07480"
     CDS             259..903
                     /locus_tag="VFQ35_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930510.1"
                     /translation="MKFGWLFVAALSIGCEKGPIPCAGVGTCPNGRECLASRCVPEGG
                     AAVPNASKRLLLRPSALGVARAHAESGGMAPGVTLGRRADNDTALYVRFDSDWNEGRV
                     ASAFLLLDPLPGSASGEDVALEVWRSRRDFVSSTFGWSVQPGLAPPFARGLARSAPPA
                     PVRVDVTELLRFFATHPSQDHGFVVRATNQSESGVTLATGIDGGAPPRLDVYLE"
     gene            complement(911..2008)
                     /locus_tag="VFQ35_07485"
     CDS             complement(911..2008)
                     /locus_tag="VFQ35_07485"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET9930511.1"
                     /translation="MGEIAFEVHRKPQPQSAAERSATMQNLGFGRIFTEHMVSVRYSA
                     ERGWHSAQLEPYAPITLDPAASVLHYGQAIFEGFKAYRQQGGGIATFRPDANARRFNA
                     SARRLAMPELPVETFVEASDVLIKNEADWVPGEMGESLYLRPLMLATEAALGVRPARE
                     YLFLLFGSPSGSYFPQGIKPLSVWLCKDFVRAAPGGTGEAKCAGNYAASLVAQQRAID
                     QGCQQVIWLDALEHRFVEEMGGMNLFFVYQQGGKTRLVTPRLTGSLLPGITRDSILTI
                     APELGYSAEEGTVSVEDWERDLASGALTEVFACGTAAVITPVGQVKFEGGSWSVNGGQ
                     TGPVAMRIRERLLAIQHGTAPDTHGWMHRVL"
     gene            2151..2762
                     /locus_tag="VFQ35_07490"
     CDS             2151..2762
                     /locus_tag="VFQ35_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9930512.1"
                     /translation="MQTRARRAAVVLFEEVELLDVAAPLQVLSVAGRRWNFRPFKLEL
                     LARNAGLVSTRNQARLEARPLSEAAPADIVIVPGGYGARRLATEEEDLRELRRICEGA
                     ELIAAIGNGVLALARAGLCSGERVAASAEICQELGTIDAVVADPRAKVVEGNRVLSAS
                     SSGSALTLGLAIVRRTMGPKLVSMVAAELGLELDEHQILEIRY"
BASE COUNT          491 a         1011 c          963 g          487 t
ORIGIN      
        1 cgacttcgca acgtgaaggc gcggcgcgac gccgagctcg tcccgaacga ccccgagcgc
       61 ctgcatccgc cgatacatgc ggccgggccc ctgcccacat cggccgagaa ggccccgcgc
      121 cccgaaattg cgggctcgcc cgaagcccct cgagcgcctg cgccacgcgc aaaaaccgcc
      181 gccgaaatcc tgctcgagcg ccgccggtcc cggtctgagc gatgatttcc gacgagccgc
      241 gatgctaaag tgcgagcggt gaagttcggc tggttgttcg tcgcggcctt gtcaatcggc
      301 tgcgagaaag gcccgattcc ttgcgcgggg gtcggcacgt gtccgaacgg gcgcgagtgt
      361 ctggcctctc gctgcgtccc cgagggtggc gcggcggtgc cgaacgcctc gaagcggctc
      421 ttgctccgcc cctctgcgct gggtgtcgcg cgcgcccacg cggagagcgg tggaatggcg
      481 cccggggtga cgctcggacg ccgcgccgat aacgacaccg ctctctacgt tcgcttcgat
      541 tccgactgga atgaggggcg ggttgcgtct gcgtttcttc tgctcgaccc attgccgggc
      601 agcgcgagcg gcgaagacgt cgctctcgag gtctggcgct cgcggcgcga cttcgtaagc
      661 tcgacatttg gctggtcggt gcagcctggg ctcgcgccac ctttcgcgcg cggactcgcg
      721 cgctcggcgc cccctgcgcc ggttcgcgtc gacgtaaccg agctcctccg attcttcgcg
      781 acgcacccat cccaagatca tgggttcgtc gtccgcgcca cgaatcagag cgagagcggc
      841 gtgacactcg cgaccggaat cgacggcggt gcaccgccgc gcctcgacgt ctacctcgag
      901 tgaccgaagc tcacagcacg cggtgcatcc agccgtgcgt gtccggcgcg gtaccatgct
      961 gaatcgcgag caggcgttcg cgaatgcgca tcgcgaccgg gcccgtttgc ccgccgttca
     1021 cgctccagga gccgccttcg aacttcacct gacccaccgg cgtaatgacg gcggcggtgc
     1081 cgcaggcgaa cacctccgtg agcgctccac tcgccagatc acgctcccag tcctcgacgc
     1141 tgaccgtgcc ctcttcagcg ctgtagccga gctcgggcgc aatggtgaga atgctgtccc
     1201 gagtgatgcc cggtaagagc gaacccgtga gccgcggcgt caccaagcgc gtctttccgc
     1261 cttgttggta gacgaagaac aagttcatgc cccccatctc ttcgacgaaa cggtgctcga
     1321 gtgcgtcgag ccaaatcacc tgctggcaac cctgatcgat tgcccgttgc tgcgctacca
     1381 ggctcgcagc gtagttgccc gcgcacttgg cttcaccggt tccgccgggc gccgcgcgca
     1441 cgaagtcttt gcagagccag acgctgagcg gcttgatgcc ttgcgggaag taagaccccg
     1501 acggcgagcc gaacagcaga aacaagtact cccgcgctgg tcgcacgccg agcgcggcct
     1561 cggtcgcgag catcagcggg cgcaagtaca ggctctcgcc catctcaccc ggcacccaat
     1621 cggcctcgtt cttgatgagc acgtccgaag cctcgacgaa cgtttccacc ggaagctccg
     1681 gcatggcgag ccgccgcgct gaagcgttga agcggcgcgc gttcgcatcg gggcgaaagg
     1741 tcgcgatgcc gccgccttgc tgacgatagg ctttgaaacc ctcgaagatc gcctggccgt
     1801 agtgcaagac ggacgccgca ggatcgagcg taatcggagc gtacggctcg agttgcgcgg
     1861 agtgccaacc gcgctccgcg ctgtagcgca cgctcaccat gtgctcggtg aaaatgcgcc
     1921 cgaaccccaa gttctgcatg gtcgcgctgc gttcggccgc gctttgaggc tggggtttgc
     1981 gatgaacctc gaaggctatt tcgcccatga tcctaagggt tcacatagca tgaaaagccc
     2041 gctcaccgag cttcgcgcgc cgcacctgaa cgcacatcgt aggccggccg tgcatgaggt
     2101 agaaccagtt ctcccccgaa ggtcgcgggt gtttcgtgta ggctcgaccc atgcagaccc
     2161 gcgctcgacg cgcagccgtc gtgctcttcg aggaggtcga gctgctcgac gtggcggcgc
     2221 ccctccaagt gctctcggta gccgggcgac gctggaattt ccggcccttc aagctcgagc
     2281 ttttggcccg caacgcgggt ctcgtcagca cgcgcaacca ggcgcggctc gaggcgcggc
     2341 ccctttctga agcagccccc gcagacatcg tcatcgtgcc cgggggctat ggagcgcgcc
     2401 ggctcgcaac ggaggaggag gacctgcgcg agcttcgacg gatttgcgag ggcgccgagc
     2461 tcatcgccgc gattgggaac ggcgtactcg cgctagcgcg ggccgggctg tgtagcggcg
     2521 aacgcgtcgc ggcgagcgcc gagatctgcc aagagctagg gaccatcgac gccgtggttg
     2581 ccgatccgcg cgccaaagtc gtcgagggaa atcgcgtgct cagcgcaagt tcgagcggct
     2641 ctgcgctcac gctcggcctc gcgatcgttc ggcgcacgat ggggccgaag ctggtctcga
     2701 tggtggccgc cgagctcggg ctggagctcg atgagcacca aatcctcgag atccgctact
     2761 gagcctgccg aaagctcact aggcgatggg aacgagcgcc ttttcccgat gcaagtcggc
     2821 agcgggagcg ccacacaagc gtcgcaccag agcgaacgca aactcgaacg cggtgccggg
     2881 cccgcggctc gtgacgatgc agccgtcttc cacgaccgaa gactcctcga agcgagcgct
     2941 cgggagctcg ta
//
LOCUS       DASTKS010000363         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_727574, whole genome shotgun sequence.
ACCESSION   DASTKS010000363 DASTKS010000000
VERSION     DASTKS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727574"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFQ35_07495"
     CDS             <1..1124
                     /locus_tag="VFQ35_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9930513.1"
                     /translation="APLGCAAHVDDEGTDSVVESAAELRGDGASSRGLSTRIIASPHP
                     VTTTDERQHLLYETFIENTGTTDVTLERLDVFAPGDPTPLVTYTRDNADNVVFPVNTT
                     TLEPFAPGAIAIVFVDVPVTRRVPQRLVQRVTATSAQGPKRGSVLEVPVDREPAITLR
                     LPLERPRLIDLGGCCDGAHSRAAMQFSDGIFVAQRFAIDFVQLSETGVETFRGDPKVN
                     SNYFLYGADVRAARAGQIVAVHDGMSENTPTEPLPTFDPNTAAGNYVIEKFDEQHFAL
                     YAHLRPGSIRVAVGDRVGAGCVLGAVGNTGNSFEPHLHFHVMDRASPLDANGLPYVFD
                     EFRLTARAELTPTGFELHETPAPNRRYRRLPMQWDVVTN"
     gene            complement(1283..1918)
                     /locus_tag="VFQ35_07500"
     CDS             complement(1283..1918)
                     /locus_tag="VFQ35_07500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9930514.1"
                     /translation="MVVDEEDVPYGEDFHDEAVAAAWADAAAKKRPFRAQIFERFAQA
                     INDSAGGEPRVLELGAGPGFLAERVLDECPRVARYTLLDFSQAMLEQSRARLARHLPK
                     AEFVRADFKSDGWFTRTTPPFDFVLSQQAVHELRHKRHAPRLYAQVRELLLPGGRLLV
                     CDHLPEGAHTPRHRVLYMSVAEHVAALEQCGFSDVQCPWSEHNMALFSATA"
     gene            complement(2040..2453)
                     /locus_tag="VFQ35_07505"
     CDS             complement(2040..2453)
                     /locus_tag="VFQ35_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930515.1"
                     /translation="MLHFRNWSAALASCCVSMLVWSLLKRCFGRYGFAVAGSLLGCSS
                     DFRAAEWLVVALTAGGAARAFWRNDARALGAVQSASLAAGFLMMLYQLGNAPWKKVDG
                     RLALALVDAGSVLLCCYVCTVAQVALAFRRQMTRR"
     gene            2573..3058
                     /locus_tag="VFQ35_07510"
     CDS             2573..3058
                     /locus_tag="VFQ35_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930516.1"
                     /translation="MRARALLSFLAAIALAGCSTYREDLNRGQRLYEENQYEHALAIW
                     RTLEPDTDSLGFNDQARYAYLRGMTDYRLGFRADARHWLAIAKAIEQEHPGGLAPEWQ
                     ERLTTSLDELNKEVYGGDKSGEVTPTAAPTSSAAPAESSVAPAPPPLSPAPSQPTSPS
                     P"
BASE COUNT          595 a         1104 c          959 g          510 t
ORIGIN      
        1 gggcaccgct cggttgcgcc gcgcacgttg acgatgaagg caccgactcg gtggtggaga
       61 gcgcggccga gctacgcggt gacggcgcga gctcgcgggg gttgtcgacg cgaatcattg
      121 catcgccgca tcccgtaacg acgacggacg agcgacagca cttgctgtac gaaaccttca
      181 tcgagaacac cggaaccacc gacgtcactc tggagcgact ggacgtcttc gctccaggag
      241 atccgacgcc gctggttacg tacacccgag acaacgccga caacgtcgtt tttccggtga
      301 acacgacgac gctggagcca ttcgcgcccg gagccatcgc catcgttttc gtcgacgtgc
      361 ccgtcactcg tcgggtgcct caacgcttgg tgcagcgggt gaccgcgacc tccgcgcagg
      421 gcccgaagcg cggctccgtg ctcgaggttc cggtcgatcg cgaaccggcg atcacgctcc
      481 gactaccgct cgaacgtccg cggctgatcg atctgggtgg gtgttgcgat ggggcgcata
      541 gtagggcggc gatgcagttc tccgacggta tcttcgttgc gcagcgcttt gcgatcgact
      601 tcgttcagct gagcgaaacc ggcgtcgaaa cgtttcgagg cgacccgaaa gtgaactcca
      661 actactttct ctacggagcc gacgtccgcg ctgcgcgcgc gggtcagatc gtcgccgtcc
      721 acgacggcat gtccgaaaat acgccgacgg agcctctgcc gactttcgac ccgaacaccg
      781 ccgctggcaa ctacgtcatc gagaagttcg acgagcagca cttcgccttg tacgcgcacc
      841 tgaggccggg cagcatccgg gtcgcggtgg gcgatcgcgt cggcgccggt tgcgtgctcg
      901 gtgccgtcgg aaacactgga aattcgttcg aaccgcacct tcacttccac gtgatggatc
      961 gggcaagccc cctcgacgcc aacggcttgc cctacgtgtt cgacgagttc cggctgaccg
     1021 cgcgcgccga gctcacgccg actggcttcg agctgcatga aaccccggcg cccaatcggc
     1081 gctaccgacg actccccatg cagtgggacg tcgttaccaa ttagtgcgcg agcgaatccg
     1141 acgactcccc atgcagtggg acgtcgtcac cgattagtgc gcgagcgaat ctgaattcgg
     1201 ggacggctct gtcgggcaaa ctgcgcgggg tgcagagccg tccatttaga gcagagcaac
     1261 tagaatgacg agccgcgctg agctacgcgg tcgcggagaa caaggccatg ttgtgctcgc
     1321 tccaagggca ctgcacgtcc gaaaacccgc actgctcgag cgcagcgacg tgctcggcga
     1381 cgctcatgta aagaacacgg tgccgcggcg tgtgcgcgcc ttcggggagg tggtcgcaca
     1441 cgaggagcct gccacccgga agcagcagct cgcgaacctg cgcgtaaagg cgcggtgcat
     1501 gccttttgtg gcgaagctcg tgaactgctt gctgcgaaag cacgaaatcg aaaggcggcg
     1561 tcgtgcgcgt gaaccagccg tcactcttga agtcggctcg aacgaactcc gccttcggaa
     1621 ggtggcgagc gaggcgcgcc cgactctgct cgagcatggc ttgcgagaag tcgagcagcg
     1681 tgtagcgcgc gacgcgcggg cactcgtcca gcacgcgctc tgcgaggaaa ccaggccccg
     1741 caccgagctc gaggacgcga ggctcgccgc ccgcagagtc gtttatcgcc tgcgcgaacc
     1801 gctcgaaaat ctgcgcacgg aaggggcgct tcttcgcggc cgcatcggcc cacgccgcgg
     1861 ccacggcttc gtcgtgaaaa tcttccccat aaggcacgtc ttcctcatcc accaccatcg
     1921 ctttacgcta gccgagtcct cgcccgcggc aagcgaactg caggccgatc caggaagtga
     1981 gctcacctca cgcgaaggag cacctcttcg acgagacgct caccgcgaga gccgaaccgt
     2041 cagcgtctcg tcatctgacg acgaaacgca agcgccacct gtgcaacggt gcagacgtag
     2101 caacagagca gaaccgaacc cgcatcgacg agggcgagcg ccaaccgacc gtcgaccttt
     2161 ttccagggcg cgttcccgag ctggtagagc atcatcaaga accccgccgc gagcgaagcg
     2221 ctttggacag ctccgagcgc ccgagcgtca tttctccaaa acgctcgggc tgcgccacca
     2281 gccgtgagtg ccacgaccag ccactcggcg gcgcgaaaat cgctgctgca acccagcagt
     2341 gaaccagcga cggcgaagcc gtaccgcccg aagcaccgct tcaagagact ccaaacgagc
     2401 atgctgacgc agcacgaggc cagagccgcc gaccagttgc gaaaatgcag catcgtccct
     2461 tcgacccctc gcgccgccga atgttccctc ctgcccgctc gcggggcggc ggagcagccg
     2521 tttccgtgcc gcccttgctt ctctcggtgt cgttacgtta gcctggccgg cgatgcgagc
     2581 tcgggctctc ctctcgttcc ttgctgctat cgccctcgcg ggttgctcca cctatcgtga
     2641 agatctcaac cgcggtcagc gcctttacga ggagaaccaa tacgagcacg cgctcgcgat
     2701 ctggcgcacc ctcgagcctg acaccgactc cctcggcttc aacgatcagg cgcgctacgc
     2761 ctatctccgc ggtatgaccg actaccgcct cggctttcgc gccgacgctc gccattggct
     2821 cgccatcgcc aaggccatcg agcaagaaca tccgggcggg ttagctcccg agtggcaaga
     2881 acgcctgacc acctccctcg acgagctgaa caaagaagtc tacggcggtg acaagagcgg
     2941 tgaggtcacc ccgaccgcag cgccgacctc cagcgccgcg ccagccgaaa gctccgtcgc
     3001 ccctgcgcct ccgcccctgt cgcccgcccc gtcccagccg acgtccccgt ccccctagcg
     3061 gcagctcgtc ccccgtcgct tcagctcgga cgccaacgcg cgacgctctg cactgcaaga
     3121 cgggctctgc gctgcaagac gggctctgca ccgcaagacg agctctgc
//
LOCUS       DASTKS010000364         3925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_506193, whole genome shotgun sequence.
ACCESSION   DASTKS010000364 DASTKS010000000
VERSION     DASTKS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506193"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFQ35_07515"
     CDS             complement(<1..753)
                     /locus_tag="VFQ35_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9930517.1"
                     /translation="MTLPAPSRIVTLLTDFGVSDPFVGIMKGVLLRDAPELQLIDVTH
                     AVPPQDVALAEFWLAESYRWFAPGTVHLCVVDPGVGSDRAALAAYADGHYFVAPDNGV
                     LGAALQADAAAEVRRIDLGKLGIAPRSNTFHGRDVFAPVAALLASGKASLRELGEAWR
                     AAVRERVPPKRAPDELVGRVVAVDHFGNLITDLPAKWLQGGFVRVEASGRTLEIVATY
                     SEAEVGECVALVSSFETLEIAVRNGNAAVALGL"
     gene            complement(750..1064)
                     /locus_tag="VFQ35_07520"
     CDS             complement(750..1064)
                     /locus_tag="VFQ35_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930518.1"
                     /translation="MVSDDQNERADGVRFELRLLKSEEGAASYRVSLRAHQREWVGDV
                     DLTPPLGEVSFHFTDSPEPPAPLLSIVRAQMRMIYRERERAGFPRRVTRWRPLPSANE
                     SE"
     gene            complement(1272..1538)
                     /locus_tag="VFQ35_07525"
     CDS             complement(1272..1538)
                     /locus_tag="VFQ35_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930519.1"
                     /translation="MVVIEPVPPASMPALPLMKPPKPLPPPCVEPAVPVLPPLGFVEP
                     PLVDEVPAPEFVLLPEALVEPEDPLLLPPEPELLLVPFEAFGFA"
     gene            1528..3768
                     /locus_tag="VFQ35_07530"
     CDS             1528..3768
                     /locus_tag="VFQ35_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9930520.1"
                     /translation="MTTKMSCSDASYSDAYMPGYQVDASVATQVSSVISSMSVADKAG
                     QMQGAPYGSDSNLDYQHIFAQPNNTTQRVAGFKFRDGPRGVNLEAGLAAGKDGYSTVF
                     PVTMARGASFDLDLELRIGDAIGDETLASGNNLMLAPAVNILRHPAWGRAQETYGEDP
                     YLLGRMGSAFTVGVQRFVPACVKHYAANNIEEQRATKISHIVDEQTLQEMYVRHFGTI
                     IQEGGVACVMAAYNQVSVGNGTPAKCTQNKQLLTDVLRTQYGFKGFVLSDWWAMPPAQ
                     ETFGQGLPNAATRASNAQGAVQAGMDMELPWNLNFTELVNDTMNSKLTVADLTTSATR
                     ILTQKFRFKVNSVDSIGGLKTPTTTLSGAGSIENNTAHLALAEEAARKGMVLLKNEGN
                     TLPIPRTVKKVAVIGADVAYKCTNTDSKAASGSVKFATDIRTGDLGSSRVLADPAKSI
                     GPFAGIQAAAGTGITVVNGTSADAAKDADFIVAVVGLTPQDEGEEYTGAGDRKSFGLD
                     AKFSSKQQDALVTALAALNKPMVVVLEGGSVIDMPWLASVKAVVMAWYPGQVGGKALG
                     QLLFGDANFSGKLPITWPKAWADEPTFSNGSSTDMDYYLGYRWFDKQAIAPLFPFGHG
                     LSYASFEYSNLQVPCTDVTQNGVINVKVDVKNTGSVKGEEVVFLFASYPGATKRRPAK
                     ELKGFTRVALDPGQAKPVTIPLRVSDLKYYDQDAHAWAPATGTVQIAVGPNAANLTLK
                     DTVTLK"
BASE COUNT          763 a         1268 c         1276 g          618 t
ORIGIN      
        1 tagcccgagc gcgaccgctg cattgccgtt acggacggcg atttcgagcg tctcgaagga
       61 cgacaccagc gcgacgcact cgccgacctc cgcctctgag taggtagcca cgatctcaag
      121 tgtgcgaccg ctagcctcga cgcgcacgaa gccgccttgg agccattttg cggggagatc
      181 ggtgatgaga ttcccaaagt ggtcgaccgc aacgacgcgg ccgacgagct cgtcgggggc
      241 tctctttggc ggtacccgct cccgaaccgc ggcgcgccac gcctcgccca gctcccggag
      301 cgacgccttg ccgctggcga gcagcgccgc gaccggcgcg aagacgtccc gcccatggaa
      361 cgtattgctg cgcggcgcga tgccgagctt tcctaaatcg atgcgccgta cctcggccgc
      421 cgcatcggcc tgcagagccg cgccgaggac gccattgtcc ggcgccacga agtagtgccc
      481 gtcggcgtac gcagcgagcg cggcgcgatc actgccgacg ccggggtcga cgacgcagag
      541 gtgcaccgtg ccgggcgcga accagcggta gctctccgcc agccaaaact ccgcgagcgc
      601 gacgtcctgc ggcggcaccg cgtgagtcac gtcgatgagc tgtaactccg gcgcgtcgcg
      661 caacaggacg cccttcatga tcccaacgaa cgggtcgctc acaccgaagt cggtgagcag
      721 cgtgacgatg cgcgacggcg cgggcagggt cattcgctct cgttggcgga ggggagcggg
      781 cgccagcgcg tgacgcgtcg cgggaagccg gcgcgctcgc gttcccggta aatcatgcgc
      841 atttgagctc gaacgatgct caggagaggc gccggaggtt caggtgaatc ggtgaaatgg
      901 aaagagactt cacccaacgg aggtgtgagg tcgacatccc ccacccactc gcgctgatga
      961 gcgcgcagcg aaacgcggta gctcgccgcg ccctcttctg acttcagcaa tcggagttcg
     1021 aagcgaacgc cgtcggcgcg ctcgttttgg tcgtcggaaa ccatgatatt aaaagattct
     1081 agcgccctct ggtggaggcg gtttcgcgct tttcagcgta taccttgggg gcttgcggcg
     1141 ggagcgcacc cgcacttgca actctttaac tggcgcggcc cgtgacaggg aacttgcatg
     1201 actcagttcg gccaccgttc ttattcgatt cacgcggtgt tttcggcctt ggctttcagc
     1261 ttagcgtgtg gtcacgcaaa gccgaacgct tcgaacggga ccaacaacaa ctcgggttcg
     1321 gggggcagca ataacgggtc ctccggctcg acgagcgctt ctggtagcaa cacgaattcg
     1381 ggggctggga cttcgtcgac gagcggcggc tcgacgaacc cgagcggtgg cagcacgggg
     1441 accgcgggct cgacgcaggg cggcggcaac ggcttgggcg gcttcatcaa cggcagcgcc
     1501 ggcatggaag caggcggcac cggctcgatg acgaccaaga tgtcgtgcag cgacgcttcg
     1561 tacagcgacg cttacatgcc gggttaccaa gtcgatgcga gcgtcgcgac gcaagtttcg
     1621 agcgtcatct cctcgatgag cgttgccgac aaggcggggc aaatgcaggg cgcgccgtac
     1681 ggctccgaca gcaacctcga ctaccagcac atcttcgcgc agccgaacaa cacgacccag
     1741 cgcgtcgcgg ggttcaagtt ccgcgacggg ccacgcggcg tgaacctcga ggcagggctc
     1801 gcggcgggca aagacggcta ttccaccgtt tttccggtca cgatggcgcg cggcgcgtcg
     1861 ttcgacctcg atctcgagct ccgcatcggc gacgcaatcg gcgatgaaac gctcgcttct
     1921 ggcaacaact tgatgctcgc gccagcggtg aacatcctgc gccacccagc gtggggccgc
     1981 gcccaagaga cctacggtga agatccttac ctgctcggcc gcatgggttc cgcgttcacg
     2041 gtcggcgttc agcgcttcgt gccggcgtgc gtgaagcact acgcggcgaa caacatcgaa
     2101 gagcagcgcg ccaccaagat ctcgcacatc gtcgacgagc aaacgctcca agagatgtac
     2161 gtgcggcact tcggcaccat catccaggag ggtggggtcg cgtgcgtcat ggccgcctac
     2221 aatcaggtca gcgtcggcaa cggcacgccc gcgaagtgca cgcagaacaa gcagcttttg
     2281 accgatgttc tgcgcactca gtacggcttc aaaggcttcg tcctgtcgga ttggtgggcg
     2341 atgccaccgg cgcaggagac cttcgggcaa ggcctgccga acgcggccac gcgcgcgagc
     2401 aacgcgcaag gcgccgtgca agccggcatg gacatggagc ttccgtggaa cctgaacttc
     2461 accgagctcg tcaatgacac gatgaactcg aagctcacgg tggcggacct cacgacgtcc
     2521 gcgacgcgca ttctgaccca gaagttccgc ttcaaggtga actcggtcga cagcatcggc
     2581 ggcttgaaga cgccgaccac gaccctgagc ggggcgggct cgatcgaaaa caacaccgcg
     2641 cacctcgcgc tcgcggaaga agcggcccga aaaggcatgg tgctcctgaa gaacgagggg
     2701 aacacgctgc ccatcccacg caccgtgaag aaagttgcgg tgatcggcgc cgacgttgct
     2761 tacaagtgca cgaacacgga cagcaaggcg gcgtccggca gcgtgaagtt cgcgaccgac
     2821 attcgcaccg gcgacctcgg cagcagccgc gtcttggccg atccagcgaa gagcatcggg
     2881 ccgttcgccg gcattcaagc ggcggccggg accggcatca ccgtggtgaa cgggacgagc
     2941 gcagacgccg ccaaagacgc ggacttcatc gtcgcggtgg tcggcctcac gccccaagac
     3001 gagggcgagg aatacaccgg cgccggtgac cgcaagagct tcggcctcga cgcgaagttc
     3061 agcagcaagc agcaagacgc gctcgtgacg gcgctcgcgg ctctcaacaa gccgatggtc
     3121 gtcgtgctcg agggcggcag cgtcatcgac atgccgtggc tcgcctcggt gaaggcggtg
     3181 gtgatggctt ggtacccggg ccaggtgggt ggcaaagccc tcggccagct gctctttggc
     3241 gacgcgaact tcagcggcaa actgcccatt acctggccca aggcgtgggc ggacgagccg
     3301 accttcagca acggcagctc gaccgacatg gactactacc tcggctatcg ctggttcgat
     3361 aagcaagcga tcgcgccact gtttccattc ggccacgggc tcagctacgc gagtttcgag
     3421 tattcgaacc ttcaggtccc ctgcaccgac gtcactcaaa acggcgtcat caacgtgaag
     3481 gtagacgtga agaataccgg gagcgtgaag ggggaagagg tcgtcttctt gttcgcatcg
     3541 tatccgggcg cgaccaagcg tcgtccggcg aaggagctca agggattcac gcgcgtcgcg
     3601 ctcgacccgg gccaggcgaa gcctgtgacg attcccctcc gcgtctcgga cctcaagtat
     3661 tatgaccagg acgcccatgc gtgggcaccg gcgaccggca ccgtgcaaat cgcggtgggc
     3721 ccgaacgcgg cgaacctgac cctaaaagac accgtcaccc tgaagtaatc gacgatgcaa
     3781 agtgccaatg agcggccggt gcgccgcgtg atgcgcccga cgactattct tcgtggggta
     3841 ggttcgaaga ccacttccgt gctcacgctg ctcgggctgc ttacgacgag cctcgcacac
     3901 gccgcggacc cgccagcgaa gcccg
//
LOCUS       DASTKS010000365         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_728334, whole genome shotgun sequence.
ACCESSION   DASTKS010000365 DASTKS010000000
VERSION     DASTKS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728334"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFQ35_07535"
     CDS             <1..592
                     /locus_tag="VFQ35_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930521.1"
                     /translation="DFLRQVNGWLKKSMAASGRAKASARFDADDERGARPSGSRLAVA
                     ERYTEAATSDPPRASGTMLIDERVVLFIDDDMETLSNFRREVQRESYTAEFALSGARG
                     LNRILSPSPPDLVVCDLTVQDLDGLSVYRAALASDAGWRSRFLFLSDTFVRRGKLELA
                     GFNGVVLEKPVQGQALRAAIRAGLSSRQPLTASGGG"
     gene            complement(578..>2134)
                     /locus_tag="VFQ35_07540"
     CDS             complement(578..>2134)
                     /locus_tag="VFQ35_07540"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF010657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HET9930522.1"
                     /translation="GLRQTGISVERIPSIAEMNDRLQRFGWGAVCVDGFIPPRAFTEL
                     QACGLLPIAADIRTREHLVYTPAPDIIHEAAGHAPILPDPGYAAYLRRFGEISSAAFT
                     LPEEQQVFDAIYALSEIKEDPAATPEQLRGADAALEVALTHAREASEATRLSRLYWWT
                     AEYGLVGRVDAYKIYGAGLLSSLWESHSCHDASVRKLPLDESCLDVSYDITRPQPQLF
                     VARDFEALHELLDAASRTLASARGGAEAFEAALRSRETCSVRFSSGAYAIGTLRDVGA
                     SLKSPGWFEFEGRVRMSSLDIAGTEAFRESSGDAAIVFSGRLQGGHRLEDLALAPPST
                     RRRFCFESGASVEGTPTRAVRRLDEKLSHIELRDVTISLPGSPRRELPEFVLFAAGDA
                     LTARAGAVDPGFYSETEFTGTRVPKPRVFSEREWALMDLYEAASRAHRGGAAAVRADF
                     PAIHRKLAQSFPDEWLLRWNLLESLLKADVDPSLRSALGSELEELELRFERKEPIASG
                     LRYLSRLAAA"
BASE COUNT          355 a          709 c          722 g          348 t
ORIGIN      
        1 ggacttcctt cgccaagtca atggatggct gaaaaagtcg atggccgcga gcggacgcgc
       61 gaaggcctcg gcgcgcttcg acgccgacga cgagcgggga gctcggccga gcggctcgcg
      121 gctggcagtc gccgagcgct acaccgaagc cgcgacgagt gatccgccac gcgcgagcgg
      181 cacgatgctc atcgacgagc gcgtcgtgct cttcatcgac gacgacatgg agacactctc
      241 gaacttccga cgcgaggttc agcgtgaatc ttacacggcc gagttcgcgc tctccggcgc
      301 tcgcgggctc aaccgcatcc tctccccgtc gcctccggat ttggtcgtgt gcgatctcac
      361 cgtgcaggat ctcgacggtc tctcggtgta ccgcgctgcg ctcgcgagcg acgcaggatg
      421 gcgtagccgc ttcttgttcc tgagcgatac cttcgtgcga cgcgggaagc tcgagctcgc
      481 agggttcaac ggagtcgtgc tcgaaaagcc ggtccagggc caggccctgc gcgcggcgat
      541 tcgcgccggt ctctcttcgc ggcaaccgct cactgcttca ggcggcggct aggcgcgaca
      601 agtagcgaag cccggaggcg atcggctctt ttcgctcgaa gcggagctcg agctcttcca
      661 gctcggagcc gagcgcgctc cgcaggctcg ggtcgacgtc ggccttgagc aggctctcga
      721 gcaagttcca ccgaaggagc cactcgtcgg gaaagctctg ggcgagcttc cggtggatcg
      781 cagggaagtc ggcgcgtacg gcggcagcgc cgccgcggtg cgctcgcgat gcggcctcgt
      841 agaggtccat gagtgcccac tcgcgctccg agaacacccg tggcttcggt acgcgcgtcc
      901 ccgtgaactc cgtttcggaa tagaagcccg ggtctaccgc accggcgcgt gcggtcaacg
      961 cgtctcctgc cgcgaagagg acgaactcgg ggagctcgcg gcgaggcgag cccgggagtg
     1021 aaatggtgac gtcgcgaagc tcgatgtgcg aaagcttttc gtcgaggcgg cggacagcgc
     1081 gggttggcgt gccttcgacg ctcgcgccgc tctcgaagca gaacctccga cgagtggagg
     1141 gtggggcgag cgctagatct tcgagccggt gaccgccctg caagcgcccg ctgaagacga
     1201 tcgcggcgtc accagacgac tcgcgaaaag cttcggtccc cgcgatgtcg agtgagctca
     1261 tgcgcacccg gccttcgaac tcgaaccaac ccggtgactt caggctcgct cccacgtccc
     1321 gcaaggttcc gatggcgtag gcgccgctcg aaaagcgcac gctgcaggtt tcgcggctcc
     1381 tcagcgcggc ctcgaacgcc tccgccccac cgcgcgccga cgccaaggtc cggctcgcgg
     1441 cgtcgagcaa ctcgtgaaga gcctcgaagt cgcgcgcgac gaagagctgc ggctgcggtc
     1501 gagtgatgtc gtagctcacg tcgaggcaac tctcgtcgag cggcagcttg cggacgctcg
     1561 catcgtggca ggagtggctc tcccacagcg acgagaggag cccggcacca taaatcttgt
     1621 aagcgtcgac gcgcccgacg agaccatact cggccgtcca ccaatagagg cgcgacagcc
     1681 gcgtcgcttc gctcgcctcg cgggcgtgcg tcagcgccac ctcgagtgcg gcgtcggcgc
     1741 ctcgaagttg ctcgggcgtt gccgcgggat cttctttgat ctcgctcagc gcatagatgg
     1801 cgtcgaagac ctgctgctcc tctggcaacg tgaacgcggc gctgctgatc tcaccgaagc
     1861 ggcggaggta cgccgcgtat cctggatccg gaaggatcgg cgcgtgccct gcggcttcgt
     1921 ggatgatatc gggcgcgggc gtgtacacca agtgctcgcg cgtgcggata tcggccgcga
     1981 tggggagcag accgcaagct tgaagctcgg tgaacgcacg cggcgggatg aacccatcga
     2041 cgcaaaccgc cccccaaccg aagcgctgca gcctgtcgtt catttccgcg atgctgggga
     2101 tgcgctcgac cgagatgccg gtctgtcgca gccc
//
LOCUS       DASTKS010000366         6392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1844606, whole genome shotgun sequence.
ACCESSION   DASTKS010000366 DASTKS010000000
VERSION     DASTKS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844606"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VFQ35_07545"
     CDS             complement(<1..819)
                     /locus_tag="VFQ35_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930523.1"
                     /translation="MTRSVVTAARRLGTGLSLVGLFVGSSLTGCGARSSLLSGEPQCR
                     REGEERPCSNPCGVGVQACEDGVWQACEVAPTSRACSDACGNGSQICEKGVWGLCEVP
                     DAQRACQNDCGNGVQRCRASVWGACEVPVTTMPCEDACGVGSRTCQNGLWSTCVGPLV
                     TEECESVCGKGKRTCDKGAWLPCDAPLPNPPVLAAVIRDFTTAHPDFERPFALGNGLE
                     TGIVEAELGPDDLPVYASSTNTRTTSGREAFDQWYRDVPGVNLSIPYTIRTTPSQ"
     gene            1124..2323
                     /locus_tag="VFQ35_07550"
     CDS             1124..2323
                     /locus_tag="VFQ35_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HET9930524.1"
                     /translation="MSSASIPPARVSVLTMSPGDAAFARFGHNAILLEWPALGTRRVY
                     NFGTFAFHGMDGVRDFMAGRFRYWLSVSTLERTLLFYGRQNRSITAQELDLDPTERAA
                     LADALEKNALPENRFYDYDYYKDNCSTRVRDAIDQLLKGELAREIRGAGRLSFRGHTE
                     RLTADAGWLYPGLDIALGPLTDRPTTRWEELFIPAELHDALARAKRHVNGQERPLVLA
                     ERELLSADREPVRSAPPARVALYGALGVVLGGAVYALGRAARTTRAARVLFGSLSALI
                     GLVLGLLGTVFVVFWAFTKHWSAYRNENILVCPPFAVALLAAGIGFALGRDRAGKLAF
                     VITRAALACALVAVLLALIPHFGQDNTRMAAFFAPLWLGLYLGTAAATGLAPWPRPRD
                     GAGRLGL"
     gene            complement(2693..4366)
                     /gene="ettA"
                     /locus_tag="VFQ35_07555"
     CDS             complement(2693..4366)
                     /gene="ettA"
                     /locus_tag="VFQ35_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET9930525.1"
                     /translation="MPEFIFTMQEVTKVHPPDKKVLENITLAFYPGAKIGVIGGNGSG
                     KSSLLRIMAGVDREFLGEARPHPGTKIGYFAQEPELGGFTYVREAVDDAVSDIKKLVT
                     EYEALSMKLGEPLSDDEMTKVLDQQAALQDRIDASDAWNLDQRVEVAMDALRLPPSDA
                     EISKLSGGEKRRVALCRILLSRPDMLLLDEPTNHLDAESVAWLERYLKEFPGTVIAVT
                     HDRYFLDDVAEWILELDRGRGYPFKGNYSGWLDQKSARLAVEEKQESSRQRKLKQELE
                     WVRASPRARQAKSKARLAAYEELVNQANNGPADAADISMPPGPRLGDLVIEADKLTKA
                     FGDRLLFDNLSFRLPRSGIVGIIGPNGAGKTTLFRLLVGEQKADGGTLKIGETVKIAY
                     VDQSRSALKPDNSVWQEISNGEEKIPLGKREVASRAYCGWFGFRGSDQQKRVGSLSGG
                     ERNRVHLAKLLKEGGNLLLLDEPTNDLDVDTLRALEEALLSFPGCAVVISHDRWFLDR
                     VATHMLAFEGDSKVVWFEGNYQAYEEDRKRRLGADADRPHRIKYRKLLG"
     gene            4488..4649
                     /locus_tag="VFQ35_07560"
     CDS             4488..4649
                     /locus_tag="VFQ35_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930526.1"
                     /translation="MDVAQTRADRGSTQIMVIGQKSLFGDLRPVLRALGLGILLGLVG
                     MLLLNALAR"
     gene            complement(4766..>6392)
                     /locus_tag="VFQ35_07565"
     CDS             complement(4766..>6392)
                     /locus_tag="VFQ35_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930527.1"
                     /translation="SSNSDEELSPTAALPRLDVPAVEPRANTASISDQVPPSPSPQAA
                     REVSVPSIPVAPPVSAPGEALAGSPDDPFIAELVDGRYRVLRLLGVGGIGLVYLCRHE
                     ALEKPVALKVLRPEYVLHEDLNARFLNEARAASAIKSPRIVDTLDVGTLPNGAPYFVM
                     EYVDGETLASLLDREGHVELSTALAIARQLAEGLDAAHAAGVVHRDLKPENVFLAHTP
                     EGLSAKIFDFGIAKVARARKRLTYAGAVFGTPHYMSPEQARGEEVDARSDLYALGVML
                     FEMVTGRVPFDGEDPLAVMSQHVDRVPPLMSNITGTPLNADLEAIVARCLAKDPRHRY
                     SSGAELLRDLEALPALMEQPLPVARAVEAPMPSAPSRLPSLQSRPETLSAHVVPAMPK
                     PRSRGRWFVAAGVVVAAAAGGAFLLRHPTSSSLTSADMGMQASVGNGPGMESSDSSSP
                     RIGVPAKAMREVHFVLSPIDSHLFRGDEDLGPMPVSVQVEEGTSMTLTVRRKGYTTRR
                     VVVDGSETRVVVGLIRAEPPQSSLSKATNRRKF"
BASE COUNT         1051 a         2108 c         2085 g         1148 t
ORIGIN      
        1 ctgagaaggc gtcgttctaa tcgtgtacgg aatcgagaga ttcacgcccg gcacgtcgcg
       61 gtaccactgg tcgaaggctt cgcgccccga ggtcgtgcgc gtgttggtac tgctcgcgta
      121 gactggcaag tcgtcgggcc cgagctctgc ttcgacgatg cccgtctcga ggccgttgcc
      181 gagcgcgaag gggcgctcga aatcggggtg cgccgtcgta aagtctcgga tgactgccgc
      241 caaaacaggc gggttcggca gcggcgcgtc acaggggagc caggcgccct tgtcgcaagt
      301 gcgctttccc ttgccgcata cgctctcgca ttcttccgta acgagtgggc cgacgcaggt
      361 gctccagagt ccgttttggc acgtgcggct cccgaccccg cacgcgtcct cgcaaggcat
      421 cgtcgtcacc ggtacctcgc acgcgcccca aaccgacgcg cggcaccgtt gcacgccgtt
      481 gccacagtcg ttttggcagg ctcgttgggc gtccggcact tcgcaaaggc cccaaacgcc
      541 cttctcgcaa atctggctac cgttgccgca agcgtccgaa caggcgcggc tcgtaggcgc
      601 tacctcacaa gcttgccaga ctccgtcctc gcacgcttga acacccactc cgcagggatt
      661 cgagcacggc cgctcctcac cttcgcgccg gcattgcggc tcgccggaaa gcagcgagct
      721 gcgcgcgccg cagcccgtca acgaagagcc cacgaaaagc cctacgagcg acagtccagt
      781 accgagtcgg cgcgcagcgg tgaccacgct tcgcgtcatg atgcccatcg tgggcggcag
      841 catacggcaa atcggcgcct cgaccagagc gccgtgccaa gtggacggct ctctaggggt
      901 ctgccaagct cgaacgtgaa tgacaggcgc gcaattgcgt cgatcgagcg attctttggg
      961 agaatctgag ctgaattcgc ggacaggccg caaagctcgg gggcgatggt cgtggccgtg
     1021 cgaggtcgag tcggcagtag tgactccggc cgcgttcggc agagccgttg gtcggtgggc
     1081 attgggggcc gcgttcggcg gagcactctt actctgcgct cgcgtgtcga gcgcctcgat
     1141 tccgcctgct cgcgtctcgg tgttgacgat gagccccggc gacgcggcgt tcgcacgctt
     1201 tggtcacaac gcgattttgc tggagtggcc cgcgctcggc acgaggcgcg tttacaactt
     1261 cggcaccttc gcctttcacg gtatggacgg cgttcgcgat ttcatggcgg gtcgtttccg
     1321 ctattggctc agcgtatcga cactcgagcg aacgctgctc ttttacggtc ggcaaaaccg
     1381 cagcatcacc gcgcaggagc tcgacctcga cccgaccgaa cgcgccgcgc tcgccgacgc
     1441 cttggaaaag aacgcgctgc cggaaaatcg cttttacgac tacgactact acaaggacaa
     1501 ttgctcgacg cgcgtgcgcg acgccatcga tcagctgctg aaaggcgagc tggcgcgtga
     1561 gatccgcgga gctggcaggc tctcttttcg tgggcacacc gagaggctca ccgccgatgc
     1621 gggctggctc tacccagggc tcgacatcgc gctcggcccg ctcacggatc gaccgacgac
     1681 gcgctgggaa gagctcttca tccctgccga gctgcacgac gccctcgcac gagcgaagcg
     1741 ccacgtgaac gggcaagaac gcccactcgt cctcgccgag cgtgagctac tttcggcaga
     1801 tcgtgagccg gtgcgcagcg ctccacccgc gcgcgtcgcg ttgtacggag cgctcggcgt
     1861 cgtgctcggc ggcgctgtct acgcgctggg ccgcgcggcg cgaacgacgc gggcagcgcg
     1921 cgtgctgttc ggttcgctga gcgcgctcat cggcttggtg ctcggcctgc tcgggaccgt
     1981 gttcgtggtc ttctgggctt tcaccaaaca ctggtcggcg tatcgcaacg aaaacatcct
     2041 cgtctgcccg ccgttcgcgg tcgccctcct cgccgccgga atcgggttcg cactgggccg
     2101 tgaccgcgcc ggcaagctcg ctttcgtcat cacgcgcgcg gcgctcgcct gcgctctcgt
     2161 cgccgtcctc ctcgcgctca tcccgcactt cgggcaagac aacacgcgca tggccgcctt
     2221 cttcgcgccg ctctggctcg gcctctacct cggcactgcc gccgcaaccg ggctcgctcc
     2281 gtggccccgc ccacgtgacg gcgctgggcg gctcggactc taacgcaacg ccgcagcggc
     2341 aatgcgcggc ttggatttaa agcaaacggc gcagagggaa gaccaagcac gcggagattt
     2401 ttttgggggg agcaacgcgg acggcgatgt ttcgcgcgga cagggttcgt ttgcgcagca
     2461 cggttgttcg ccgccaagac gcccaaggtt cggaagcaaa gcgcgcggag ggttttgggg
     2521 gtgcagcggg gacgccgacg ttgcgctggt cgcgctcgtc agccagcagc gcggccaagt
     2581 tcacgttgcg ccgctcactc cccgaaaaat gcccggtgcg gctcgaatcc gttcggtgcc
     2641 ccagcgttga accagcgctc aaaagcaagc gcccgctggg cgcgcgcccg tttcagccga
     2701 ggagcttccg gtatttgatg cggtgcgggc ggtcggcgtc cgctccgagg cggcgctttc
     2761 ggtcttcttc gtaggcttgg tagttgccct cgaaccaaac gactttgctg tcgccctcga
     2821 atgcgagcat gtgcgtcgct acgcgatcca agaaccagcg atcgtggctg atcaccaccg
     2881 cgcaaccggg gaaggagagg agcgcttctt cgagagcgcg caacgtgtcg acgtcgaggt
     2941 cgttggtcgg ctcgtccaag aggaggaggt tgccgccctc tttcaggagc ttggcgaggt
     3001 gcactcggtt gcgctcgccg cccgagaggc tgccgacgcg cttttgctgg tcactaccgc
     3061 ggaaaccgaa ccatccgcag taggcgcgtg acgcgacttc gcgcttgccg agcgggatct
     3121 tctcctcgcc gttcgagatt tcctgccaca cgctgttgtc cggtttgagt gcggagcgcg
     3181 actgatccac gtacgcgatc ttgacggtct cacctatctt caaggtgccg ccgtccgcct
     3241 tctgctcacc gaccagcaag cggaacaaag tcgttttgcc ggcgccgttc ggcccgatga
     3301 tgcccacgat tccgctgcgc ggcaggcgga aactcaggtt atcgaacagc aagcgatcgc
     3361 cgaaggcttt ggtgagcttg tcggcttcga tcacgagatc accgaggcgt ggcccaggcg
     3421 gcatgctgat gtcggccgcg tccgcaggtc cgttgttggc ttgattgacg agctcttcgt
     3481 aagcggccaa gcgcgccttg ctcttcgcct gacgagcgcg cggtgacgca cgcacccact
     3541 cgagctcttg tttgagtttg cgttgacgcg agctctcttg cttctcttcc accgcaagac
     3601 gcgcggactt ttgatcgagc cagccagagt agttgccctt gaagggatac ccacgacccc
     3661 gatcgagctc caaaatccat tcggccacgt cgtcgaggaa gtagcgatcg tgggtcaccg
     3721 cgatcacggt gccggggaac tctttcaagt agcgctcgag ccaggccacc gactccgcgt
     3781 cgaggtggtt cgtgggctcg tccaagagca gcatgtcggg gcggctcagc aagatgcggc
     3841 agagcgcgac gcggcgcttc tcaccaccgg agagcttgga gatttccgcg tcgctcgggg
     3901 gcaggcgcag cgcgtccatc gccacttcga cgcgctggtc caggttccaa gcgtcggatg
     3961 cgtcgatgcg atcttgcagc gccgcttgct ggtccaaaac cttcgtcatt tcgtcgtccg
     4021 agagcggctc gccgagcttc atgctcagcg cttcgtactc ggtgacgagc ttcttgatat
     4081 cggacaccgc gtcgtcgacg gcctctcgca cgtacgtgaa ccccccgagc tccggctctt
     4141 gcgcgaagta gccgatcttc gtgccggggt gcgggcgagc ctcgcccaag aattcgcgat
     4201 cgacgccggc catgatgcgc aacagcgagc tcttgcccga gccgttgccg ccgatgacgc
     4261 cgatcttcgc tcccggatag aaggcgagag tgatgttttc gaggaccttc ttgtcgggag
     4321 gatggacctt cgtcacctcc tgcatcgtga aaatgaactc gggcatggtc ggaacctttt
     4381 acctcagttc ggcacacgag ttggcacagt gacggctcgg ccatccgcag ttttccgaga
     4441 aaacacggcg cgtcgcgctg gcacgtgcgt tgcttggagc atcccccatg gatgtggcgc
     4501 aaacccgggc ggacaggggg tccacccaaa tcatggtgat cggtcaaaag agcctatttg
     4561 gcgatcttcg gcccgtgctg cgagccctgg ggctcggcat cctgctcggc ctggtcggca
     4621 tgctccttct gaacgcgctc gcgcggtgac ggcgccagtc acgcagcggt ggtcgccgac
     4681 cgccacggtg accggcaatt ggcccgaggc cgcgcgggtt ttacggttcg ttacggactg
     4741 acgccgcttc ccgctcgtca gccgctcaga actttcggcg attcgtcgct ttactgaggc
     4801 tgctctgcgg cggttccgcg cgaatgagcc cgacgacgac gcgggtctcg gagccatcga
     4861 ctacgactct ccgcgtcgtg taccccttgc gacgaacggt gagggtcatc gaagttccct
     4921 cctcgacctg caccgagacg ggcatgggac cgaggtcttc gtcaccgcga aacaggtgcg
     4981 agtcgatcgg cgagagcacg aaatggactt cgcgcattgc cttcgcgggc actccgatac
     5041 gcggcgagga agagtcggac gactccatcc cggggccgtt gccgaccgag gcttgcatgc
     5101 ccatgtcggc gctcgtcagg ctgctcgagg tggggtggcg caacagaaag gctcccccgg
     5161 ctgccgccgc aacgaccacg cccgcagcga cgaaccaacg gccgcggctg cggggcttgg
     5221 gcatcgcggg aaccacgtga gccgacagcg tttccggccg actctgcaag ctcggtaggc
     5281 ggctcggcgc ggatggcatc ggcgcttcga ccgccctcgc gaccggcaac ggctgctcca
     5341 tcaacgcagg caacgcctcg aggtcgcgca gcaactcggc gccgctgctg tagcggtgcc
     5401 gaggatcctt ggcgaggcag cgcgcgacta tcgcctcgag atccgcgttg agcggagtgc
     5461 cggtgatgtt cgacatcaac ggcggcaccc ggtcgacgtg ctgcgacatc accgcgagcg
     5521 ggtcttcacc gtcgaacggt actcgaccgg tgaccatctc gaacagcatc acgccgagcg
     5581 cgtacaagtc cgagcgcgca tcgacttctt caccgcgcgc ctgctcgggc gacatgtaat
     5641 gcggcgtacc aaacacggcg cccgcatacg tgagccgctt gcgcgctcgg gcgaccttgg
     5701 cgatgccgaa atcgaagatc ttggcggaca agccctcggg cgtgtgcgcc agaaagacgt
     5761 tctctggctt gagatcgcgg tggacgacgc ccgcagcgtg cgcggcgtcg agcccttcgg
     5821 cgagctggcg agcgatggcg agcgcagtcg agagctcgac gtggccctcg cgatcgagca
     5881 gcgatgcgag tgtctcgccg tcgacgtact ccatcacgaa gtacggagcg ccgttcggca
     5941 gcgtaccgac gtcgagggtg tcgacgatgc gcgggctctt gatggcggag gcggcgcgcg
     6001 cctcgttcaa gaaccgcgcg ttcaggtcct cgtgcagcac gtactccggg cgcagcactt
     6061 tgagggcgac cggcttttcg agcgcctcat ggcgacagag gtacacgagc ccgatgccgc
     6121 cgacgcccag gagccgcagc acccgatagc gaccatccac gagctcagcg atgaacgggt
     6181 cgtccggact ccccgcgaga gcttccccgg gggccgaaac cggcggtgca acgggaatcg
     6241 acggcaccga aacctcacgc gctgcctgcg ggctgggcga tggcggcacc tgatcagaaa
     6301 tgctggcggt gttggcccga ggttcaacgg ccggcacgtc taggcgcggc agcgctgccg
     6361 tgggcgagag ctcttcgtcc gagttcgacg ag
//
LOCUS       DASTKS010000367         6945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1621992, whole genome shotgun sequence.
ACCESSION   DASTKS010000367 DASTKS010000000
VERSION     DASTKS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621992"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3085
                     /locus_tag="VFQ35_07570"
     CDS             <1..3085
                     /locus_tag="VFQ35_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930528.1"
                     /translation="TYREVLELDGGNLTALRGLERICERVNDWPDLVGVLERQLDVVE
                     TERERVEVLLKLAQIQEEHFLKPDLAAQRLEQAVEISPGEERAYVALARCYRRLKQWL
                     DLINTYERHIAETSSTQTRVELYGQMATIFADEVGDADRAIDAYRNIVDLDDTNVPAL
                     EALSKLYEKQGDTSQAIESMSRVADLTTDGKQRVEMYYRIGKALDEKLGDRQSAQERF
                     EMALDLDPSHLPSLAALRTIAIDESDWDRAARYLEQEQLNTQAPRARARLLVELGKLR
                     DEMLSEHDLAVQAYELAIQCDEDCEEAALPLVEEYARTARWAQAEPLAEMLVRKSKNK
                     DRSEQHTLYKLLGKVHAALGNYEKALKAYQTSNQLDLTDQESIRGVADVAFELRDWPT
                     ALTNYQKVLTALAEEDTEARTDVYYRLGCIKREQGQARQAINNFEKALALVSDHRPTL
                     EALIGVYAKSNDWKQVAAYKRQILDSVTDGEERFRILNEIGEIWSDREKNPQKAIEAL
                     EEALDFKPRDHVLLHKLLQLYQAASDWPKMVSSLDQIASLEEKPELRARYLFTQAQIY
                     RDKIQDVDRAVELFNEALDLNPGYLEAFERINKVLTQQKNWKQLERSYRKMLHRVAGK
                     GNLELEHTLWHQLGVIYRDRLQQTEPAIEAFRMAASVTPGQILDNQILAELYEVSERW
                     DEAIEEQRVILTADPLKVDAYRALYRLYLQKQAYDEAWCLAAAMVFLKKADAEETRFF
                     EDYRPQGMLAVKGRLNNDLWIKHVFHEDENIHVSQIFAMIAPAALAAKIAMQQAQNKA
                     PVLDKRFRQDPLTSTVTFAKTFGWASQVLGVQPPELYVRNDVPGAIIAVAANPRASVA
                     GQTVLTGFSPPELTFMCGRHLAYYREEHYIRTLFPSQAELTIMLFAGVMLANPSTPMP
                     QDMAAQIRATAQELAKYMQPIHLDGLRAVVKRFLDDGAKANIKRWNQAVEISSCRAGL
                     IVCGDLDIPRKILAAEPQLPGDASPADKMKELLTYFVSEHYFEVRRALGITIATE"
     gene            complement(3406..4086)
                     /locus_tag="VFQ35_07575"
     CDS             complement(3406..4086)
                     /locus_tag="VFQ35_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930529.1"
                     /translation="MFGPRIACALVLLVASPAIAAQPEPAAAQPATDTSEPAEEEQTP
                     LIPAAKDQLSGHLLLGASGLGLLPIAMLDGKTSFMDRAGLGVGAAGDLGIGVSRNVVF
                     GIFGEYARFTSSSDCSSCDTDTLAVGPFVRYHVVQGTRFDPWLMLGVGYRRLTASGGP
                     KEADYRGIDWARLALGGDWYALSQVGFGPYAEVTLGTFTDRPSGTSATVYGMVNLGVR
                     LAFDLQGR"
     gene            4160..5437
                     /locus_tag="VFQ35_07580"
     CDS             4160..5437
                     /locus_tag="VFQ35_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9930530.1"
                     /translation="MKTAPGTCPENSNTVELACVSPPGPWLLEVGAPEGGHARILHVG
                     DRIVIGSGTHADVRVFDPAVSQRHCRLEVLPTHIELEDLGSKNGTYVGAVRIGRASVA
                     HAGVFVVGRSSVVVHRGGEEEVPAADSVPGLVGSSLGMQRVAAEIRRHARTRAPILLQ
                     GESGTGKDVVARTLHTLSRRQGTYVPLNVGALPETLADAELFGHRRGAFTGAVQSRAG
                     AFEQADGGTLFLDEIADLPLAIQVKLLRVVEDGMLRPIGAHSSQRVDVRIVSASWAII
                     EQRVEAGAFRGDLYHRLSTVTIQIPPLRSRKSDIPELSCSLLRRIRDEVGEKELSSGA
                     LSRLMDYDFPGNVRELGSVLYRAAMASPEREILAQHVQVGRAKTLPRVATLGSDDAKM
                     LLDQHHGNVSRAARAAGVPRSTFRSWLGRTPGA"
     gene            complement(5530..6771)
                     /locus_tag="VFQ35_07585"
     CDS             complement(5530..6771)
                     /locus_tag="VFQ35_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930531.1"
                     /translation="MRQPTCDCSMSLSTKLPKQMRLRSALAANARRFVLGGMLAMCIT
                     RPASADDEATTSIARERFKEGVQFFDQKQFEKARAAFLQAYALKRHPAVLLNLAQSEL
                     RSGHEADAAKHFAQYLREAKEATEAERQAADSGLSAAKAVVAEVAVVVDEDGAVVSVD
                     GTAEGQTPLPGPVYLSPGAHTLSAKKDARETSQSVTAVAGQSTSTNLRFRKASPAADA
                     GERQKPENTAKPPTPVSPPVSNTPVEPAPSEGSARRRQGFFEWASDSPVAWIGGGVTA
                     LGLAGGIGFALGAKHNYDNADETAARIATEAKKDGLSSTKGLCVGRADSMIPSNRLAD
                     YREACGLYSDYVDKGDKMKTLSIVSWVVAGTAAATTLIVYAASGTVPAKETAQRSRGF
                     KAHVAPWISGTERGVFVGGSF"
BASE COUNT         1257 a         2278 c         2297 g         1113 t
ORIGIN      
        1 gacctatcgc gaggtgctgg agctcgacgg cggcaacctg accgctctgc gtggcctcga
       61 gcgcatctgc gagcgggtca acgactggcc cgatctcgtg ggcgtgctcg agcgccagct
      121 cgacgtcgtc gagacggagc gcgagcgcgt cgaggtgctg ctgaagctcg cccaaatcca
      181 ggaggagcac ttcctcaagc cggatttggc ggctcagcgc ctggagcagg cggtcgagat
      241 ttcgccggga gaggagcgcg cttacgtcgc gctcgctcgc tgctaccgcc ggctcaagca
      301 gtggctcgac ctcatcaaca cctacgagcg acacatcgcc gagacgtcga gcacgcagac
      361 gcgcgtcgag ctctacgggc aaatggccac gatcttcgcg gacgaggtgg gtgacgcgga
      421 tcgcgccatc gacgcttatc gcaacatcgt cgacctcgac gacacgaacg tccccgcgct
      481 cgaggcgctc agcaagctct acgagaagca gggcgacacg tcgcaagcca tcgagtcgat
      541 gtcgcgcgtg gccgacctca cgacggacgg caagcagcga gtcgagatgt actaccgcat
      601 cggtaaggca ctcgacgaga agctcgggga tcgtcagtcg gcgcaagagc gcttcgagat
      661 ggcgctcgac ctcgacccgt cgcacctacc gagcctcgcg gccctccgca cgatcgccat
      721 cgacgagtcc gattgggacc gcgctgcgcg ctacctcgag caagagcagc tgaacacgca
      781 agcgccccgc gctcgcgcgc ggctcctggt cgagctcggc aagctgcgcg acgagatgct
      841 gagcgagcac gacctcgccg tgcaggccta cgagctcgcg attcagtgcg acgaggattg
      901 cgaagaggcg gcgttgcccc tcgtggaaga gtacgcgcgg acggcgcgct gggcgcaagc
      961 cgagccgctc gccgagatgc tcgtccgcaa gagcaagaac aaggaccgct ccgagcagca
     1021 caccctctac aagctgctcg gtaaggtgca cgcggcgctc ggcaactacg agaaggcgct
     1081 caaggcgtac caaacctcga atcagctcga cctcacggat caggagagca ttcggggcgt
     1141 cgccgacgtc gcgttcgagt tacgcgattg gccgaccgcg ctcacgaact accagaaggt
     1201 gctcaccgcg ctcgccgagg aagacaccga ggcgcgcacc gacgtctact accggctcgg
     1261 ctgcatcaag cgcgagcagg ggcaggcgcg ccaggccatc aacaacttcg agaaggcgct
     1321 cgcactggtc agcgatcacc gcccgacgct cgaggcgttg atcggcgtct acgcgaagag
     1381 caacgactgg aagcaagtcg cggcctacaa gcgccaaatc ctcgatagcg tcaccgacgg
     1441 cgaggagcgc ttccgcattc tgaacgagat cggcgagatc tggtcggatc gcgagaagaa
     1501 cccgcagaag gcgatcgagg ccctcgagga ggcactcgac ttcaagccgc gcgatcacgt
     1561 gctcttgcac aagctcttgc agctctacca ggccgcgtcc gactggccga agatggtgag
     1621 ctcgctcgac cagatcgcct cgctcgaaga gaagcccgag cttcgcgcgc gctacctctt
     1681 cacccaagcg cagatctacc gcgacaagat tcaggacgtg gaccgcgcgg tcgagctctt
     1741 caacgaagcg ctcgacttga accccggata cctcgaggcg ttcgagcgca tcaacaaggt
     1801 gctcacccag cagaagaact ggaagcagct cgagcgctcg taccggaaga tgcttcaccg
     1861 cgtcgccggt aagggcaacc tagagctcga gcacaccttg tggcaccagc tcggcgtcat
     1921 ctatcgcgat cgcctgcagc aaacggagcc tgcgatcgag gcgttccgca tggcggcgtc
     1981 ggtcacgccg gggcagatcc tcgacaacca aatcctcgcc gagctctacg aagtgagcga
     2041 acgctgggac gaggcgatcg aggagcagcg cgtcatcctg accgcggacc cgctcaaggt
     2101 ggacgcctac cgcgctctct atcgcctgta tctacagaag caggcttacg acgaagcgtg
     2161 gtgcctcgcg gccgcgatgg tcttcttgaa gaaggccgac gccgaggaga ctcgtttctt
     2221 cgaggactac cgcccgcaag gcatgctcgc cgtgaagggt cgcctgaaca acgacctttg
     2281 gatcaagcac gtcttccacg aggacgagaa catccacgtc tcgcagattt tcgcgatgat
     2341 cgcgcccgcc gcgctcgccg ccaagatcgc gatgcagcag gcgcagaaca aggcgccggt
     2401 tctcgacaag cggttccgtc aggatccgct cacctccacg gtgacgttcg cgaagacctt
     2461 tggttgggcg tcgcaggtac tcggcgtgca gcctccggag ctctacgtcc gcaatgacgt
     2521 ccccggggcg atcatcgcgg tcgcggcgaa cccgcgcgcg tcggtggcgg ggcagacggt
     2581 gctcacgggc ttctcaccgc cggagctcac cttcatgtgc ggtcggcacc tcgcgtacta
     2641 ccgcgaggag cattacatcc gcacgctgtt cccgtcgcag gccgagctca ccatcatgct
     2701 ctttgcgggc gtgatgctgg cgaatccgag cacgcccatg ccgcaagaca tggccgcaca
     2761 gattcgcgcg acggcgcagg agctcgcgaa atacatgcag cccatccacc tggatggttt
     2821 gcgtgcagtc gtgaagcgct tcctcgacga cggtgccaag gcgaacatca agcgctggaa
     2881 ccaggcggtc gagatttcgt catgccgcgc tggcctcatc gtgtgcggcg acctcgacat
     2941 cccgcgcaag atcttggcgg cggagcccca gctgcccggc gatgcgtcgc cggcggacaa
     3001 gatgaaggag cttctcactt acttcgtgag tgagcactac ttcgaggtcc gtcgcgcgct
     3061 cggcatcacc atcgcgacgg agtgagctcg aaggcgaggg tctgcggcgc gcgaacaacg
     3121 cgccgcggcg cctcacgacg gagtgagctc gaaaggcgag cgtttgcggc gcgcgaacaa
     3181 cgcgccgcag cgcgctaacg acgagtgagc tcgaaggcga gcgtttgcgg cgcgcgaaca
     3241 aacgcgccgc ggcgcgctca cgacggagtt aaagcgaacg tttcgacgcg ccgcatcgcg
     3301 ggcgcgaggg agcgagctcg aagcgaagag cgcccctcga gagcccagcg agctcgaagc
     3361 gaagagccgc gcggctcgag agcccagcgc gctcgtgggt agaggtcagc gcccctgtag
     3421 gtcgaacgcg aggcgaacac ccaagttcac catgccgtac acggtcgcgc tggtgccgct
     3481 ggggcggtcg gtgaacgtgc cgagcgtgac ctccgcgtac gggccgaacc cgacttggct
     3541 gagcgcgtac cagtcaccgc cgagagcgag gcgcgcccaa tcgataccgc ggtagtcggc
     3601 ctctttcggg ccgccgcttg cggtgagcct gcggtaccca acgccgagca tgagccacgg
     3661 atcgaagcgc gttccctgaa cgacgtgata gcgaacgaat ggtccaaccg cgagcgtatc
     3721 ggtgtcgcag ctcgagcagt cggacgagct cgtgaagcgg gcgtattcgc cgaatatgcc
     3781 gaataccacg ttgcgactaa cgccaatgcc cagatcgccg gccgccccga cgccgagacc
     3841 ggcgcgatcc atgaagctcg tcttgccgtc cagcatggcg attggcagca atccgaggcc
     3901 gctcgccccg agcaacaggt gccctgagag ctgatcctta gcggcgggga tgagcggcgt
     3961 ctgctcctcc tcggcgggtt cggacgtatc ggtcgcgggc tgggcagcgg ccggctccgg
     4021 ctgggccgcg atggcgggcg aggcgacgag gagcacgaga gcgcaggcga tacgcggacc
     4081 gaacatggct cgccacttag caccgccgtc gcgtgttgga aaaccggaaa cccgcgccgc
     4141 cgctggccga tggaggaaca tgaagacggc ccccggtacg tgccccgaaa acagcaacac
     4201 ggtcgagctc gcctgcgtct cgccgcccgg cccctggctg ctcgaggtcg gggcgcccga
     4261 gggaggccac gctcgcatcc tgcacgtcgg tgaccggatc gtcatcggga gcggcaccca
     4321 tgcggacgtg cgcgttttcg acccggccgt gagtcagcgg cattgccggc tcgaggtcct
     4381 cccaacgcac atcgaactcg aggatctcgg ttcgaagaat ggcacttacg tcggggcagt
     4441 ccgcatcggt cgggcaagcg tcgcgcacgc cggtgtcttc gtcgtcggac gtagcagcgt
     4501 ggtggtgcac cgcggcggcg aagaggaggt gcccgcggct gacagcgttc caggtctggt
     4561 cggaagctct ctcggcatgc aacgggtcgc ggccgagatc cgccggcacg cgcgcactcg
     4621 agcgccgatc ctgcttcagg gcgagtccgg aacgggcaag gacgtcgtcg cgcggactct
     4681 gcacacgctg agtcggcgcc agggcacgta cgttccgctc aacgtgggcg cgcttccgga
     4741 gacactcgcg gacgcggagc tcttcgggca tcggcgtggc gcgtttacgg gagccgtgca
     4801 gagtcgcgcg ggtgcattcg agcaagccga cggtggcacc ctttttctcg acgaaatcgc
     4861 ggatcttccg ctggctattc aagtgaagtt gctgcgcgtc gtcgaagacg ggatgttgcg
     4921 gcccatcggc gctcacagct ctcaacgcgt ggatgttcgt atcgtctcgg cgagctgggc
     4981 catcatcgag caacgcgtcg aggcgggcgc gttccgcggc gatctgtacc atcggctgtc
     5041 caccgtaacg attcagatcc ctccgctcag aagccgcaag agcgacattc cggagctctc
     5101 gtgctccctg ttgcggcgca tccgcgatga agtcggtgaa aaggagcttt cgagcggagc
     5161 gttgtcgcgc ctcatggact acgacttccc cggcaacgtg cgcgagctag gcagcgtctt
     5221 gtaccgcgcc gccatggcca gcccggaacg cgaaatcctc gctcagcacg tacaagtcgg
     5281 gcgcgcgaag acgttgccgc gagtcgccac cttgggctcc gacgacgcca aaatgctgct
     5341 cgaccagcat cacggaaatg tgagccgcgc cgcgcgtgct gccggcgtgc cacgcagtac
     5401 gtttcgctcg tggctcggtc gcacgccggg cgcgtgaccg gtcgcgtttc gcagaagaca
     5461 gcgccgcagc gctcggcctc agaatgaagc tcgggggcgg ctcagaaggg gcggctcaga
     5521 aggggcggct cagaagctgc cacccacgaa gacgccgcgc tcggtgcccg aaatccacgg
     5581 agcgacgtgc gctttgaacc cgcgcgagcg ctgggcggtt tccttcgccg gcaccgtccc
     5641 gctcgccgcg tacacgatga gggtggttgc ggcggccgtg cccgcgacga cccagctcac
     5701 gatcgagagc gtcttcatct tgtcgccctt gtcgacgtag tccgaataga gcccgcacgc
     5761 ttcgcgatag tcggcgaggc gattcgaggg aatcatcgag tccgcccggc ctacgcatag
     5821 gcctttggtc gaagagagcc cgtccttctt cgcttcggtg gcgatgcgag ctgcagtctc
     5881 gtcggcattg tcgtagttgt gcttggcgcc gagggcaaag ccgataccgc cagcgaggcc
     5941 gagcgccgtg actccgccgc caatccacgc aaccggggag tcggaggccc actcgaagaa
     6001 gccttggcgc ctgcgtgcgc tgccttcgga cggcgcgggc tcgaccggcg tgttcgacac
     6061 gggcggggac actggcgttg gcggcttcgc cgtgttctcc ggcttctgcc gctctcccgc
     6121 gtccgctgca ggggaagcct tccggaatcg caggttggtc gatgtcgatt gtcccgcaac
     6181 cgcggtgacc gactgagagg tctcgcgcgc gtccttcttg gcgctgagag tgtgagcacc
     6241 gggcgacagg tatacggggc cgggcagcgg cgtctggccc tccgcggttc cgtcgaccga
     6301 aacgacagca ccgtcctcat ccaccacgac cgcgacttca gcgaccaccg ccttcgccgc
     6361 ggagaggccc gaatcggctg cttgtcgttc ggcctctgtc gcttccttgg cttcgcgcag
     6421 atattgggcg aagtgcttgg ccgcgtctgc ctcatgcccg gaacgtagct cgctctgcgc
     6481 gaggttgagc aacaccgccg ggtgacgctt cagcgcgtac gcctgcaaga acgcggcgcg
     6541 cgccttttcg aactgctttt gatcgaaaaa ctgtacgcct tctttgaagc gttcgcgcgc
     6601 gatgctcgtc gtggcctcgt cgtccgccga tgccgggcga gtgatgcaca tcgcaagcat
     6661 gccgcccaac acgaatcgcc tggcgttcgc cgcgagcgca gagcgcagcc tcatttgctt
     6721 cgggagtttg gtggagaggc tcatcgagca gtcgcaggtc ggttgtcgca tccccatgct
     6781 ttctagtctc ggacgggctg actcagaaag gagagtctcg aatttcagaa gggagaatct
     6841 cgcacgataa cccctttacc tttcgcaggc gccgcgggtt gagtcgtggt gcgcggcttg
     6901 ggtgaagggg cagctagcgt cttcttggcc ggtgccggct tggcc
//
LOCUS       DASTKS010000368         3946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1399278, whole genome shotgun sequence.
ACCESSION   DASTKS010000368 DASTKS010000000
VERSION     DASTKS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399278"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VFQ35_07590"
     CDS             <1..856
                     /locus_tag="VFQ35_07590"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET9930532.1"
                     /translation="LPVVRRLFPISHVERNLDQLEAMLARETDYSHERSNLARMREIF
                     RAREDVVIPEVLDELTGGGVLAMSFEEGLKVTDLEAQRQSGIDPEAVARLLVEAYFVM
                     LFDHGVFHADPHPGNFLVRPGPKLVVLDFGAVEDVTPALSEGMKMVVLGAITRNDDQI
                     LRGLERMGFVAEGGDRELLAQVGREYLKVLSGVRIEDFSKIDRQVVEKLTGYQQIRGK
                     LREVMRSVEYPEGYFYVERTLALLFGLVGQLAPKVGLPGLVLPHASKAFARGLAMGRP
                     KPAEAPPA"
     gene            853..2262
                     /locus_tag="VFQ35_07595"
     CDS             853..2262
                     /locus_tag="VFQ35_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930533.1"
                     /translation="MNRAAFALLLAGCTPAVAPRGFGTSPVAECDYVVHVESSRPVRL
                     GVSATCRGRAVQGLESSDSRVASAIGSVTSDRGETVRDGDSFLLRTPAREVTFRYRID
                     LDALAEGHHRALARRSGESLLAPTSSYLMYPWPLDVGIPVRVRFEAPPELAVSSGLER
                     EGDHYRLEAHEIPVATYSAFGKLARDVVPIDDAREELDVTVLDGELAVNEQRITRWAA
                     ERARAVSAFYRGFPAARASLFVVPVPNRREVVFGNLLPESSPAAVLEVGSLTNDAALD
                     DDWVLVHELFHIGVPSFHGEGKWFDEGLATYFEPIIRVRSGLLEPLNAWREFALTMPR
                     AERALTRDGLEQSDDIYWAGAIFCLLADVTAREQSQGRQGLEDGLRKVRAAGGNASEV
                     WPLKKTLEIADSAFAQPVLVPLWKRYARKPAPLDLAALFEALGVTRDGSGVHLSDAAP
                     RAWVRRSIFEPGAAISASR"
     gene            complement(2246..3106)
                     /locus_tag="VFQ35_07600"
     CDS             complement(2246..3106)
                     /locus_tag="VFQ35_07600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9930534.1"
                     /translation="MSTSEGSSVSDCWRDRSGQAWVREEARIDKTLVPFGRAALESLS
                     LAAGQSVIDVGCGTGQTLVEIAGRVGPSGKVLGVDVSEVMVERARQRLAASGRANVEV
                     ELADAQSHSFEPARYDAVFSRFGVMFFENTAAACMNLVSALKRGGRLAFVCWQSIERN
                     PWAFLPASAVREQLRMEALPDFLTPGRPGPFRFADPEPLGEALMLAQCQTVEFKALEL
                     KVHLGGAETLEQAVAYSLQVGPGARLLGDADAATKDAVAATLRKVFAPFVTESGVFMD
                     ARAWLVSGSR"
     gene            complement(3153..>3946)
                     /locus_tag="VFQ35_07605"
     CDS             complement(3153..>3946)
                     /locus_tag="VFQ35_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HET9930535.1"
                     /translation="PCAWPRVYNAGEMELGVQAIALLAAAAFGAGLVDAIGGGGGLVT
                     VPALLAAGLGPHAVLATNKGQASFGATASFATYFRRGGLDRSKVPWGLASGFIGSLAG
                     AELVSLIPPAPLRPLVLVLLGAAAVVMILREHLLRPSAPNSDGKWGVVALGLAGGAYD
                     GFFGPGTGTLLIAGFVHFFGDSPTRASGNAKVINFGSNIAALLVFQLRGSVAWPVALP
                     MGVANWVGANIGARLALRHGDRTVRVVVLSVAAAVTIKVALDLLS"
BASE COUNT          683 a         1340 c         1310 g          613 t
ORIGIN      
        1 cctgccggtc gtgcgcaggt tgttccccat ttcgcacgtc gagcggaact tggaccagct
       61 cgaggcgatg ctggcgcgcg agaccgacta ctcgcacgag cgctcgaacc tcgctcgcat
      121 gcgcgaaatc ttcagggcgc gcgaggacgt ggtcatccca gaggtcctcg acgagctcac
      181 cggcggcggc gtgctcgcga tgagcttcga agagggcctc aaggtgaccg atctcgaggc
      241 gcaacgtcag agcgggatcg atccggaggc ggtcgcgcgg ctcttggtcg aggcgtactt
      301 cgtgatgctg ttcgaccacg gcgtgtttca cgccgatccg caccccggta acttcctggt
      361 gcgacccggg ccgaagctcg tcgtgctcga cttcggggcg gtggaggacg ttacgccggc
      421 gctcagcgaa ggcatgaaga tggtggtgct cggcgccatc acgaggaacg acgatcagat
      481 cctgcgaggg ctcgagcgga tgggcttcgt cgcggaaggc ggcgaccgcg agctcctggc
      541 gcaggtggga cgcgagtact tgaaggtgct ttccggcgtg cgcatcgaag acttctccaa
      601 gatcgatcgc caagtcgtcg agaagctcac cgggtatcaa cagatccgcg gcaagctccg
      661 cgaagtgatg cgcagcgtcg agtatccgga aggctacttt tacgtcgagc gcacgctggc
      721 gctgctcttc ggactcgtcg gtcagctcgc gccgaaggtg gggcttcccg gattggtctt
      781 gccccacgcc tcgaaggcgt tcgcgcgggg gctcgccatg ggccgcccca aaccagccga
      841 agcgccgcca gcatgaaccg cgcagctttc gcgcttctgc tcgctggatg cacgcccgcg
      901 gtcgcgccac ggggtttcgg cacgagcccg gtcgccgaat gcgactacgt ggtgcacgtc
      961 gagagcagcc gtcccgtccg cctcggggtg agcgcgacgt gccgcggacg cgccgtccag
     1021 ggcctcgaaa gcagcgattc gcgcgtcgcg agcgcgatcg gctcggtgac gagcgaccgc
     1081 ggcgagacgg tgcgcgacgg ggacagcttt ctgctccgaa caccggcgcg cgaagtgacg
     1141 tttcgctacc gaatcgactt ggacgcgctc gccgagggcc accatcgcgc gctcgcgcgt
     1201 cgcagcggcg aatcgttgct cgcgccgacg tcgagctacc tcatgtatcc gtggccgctc
     1261 gacgtcggta ttccggtgcg ggtccgcttc gaggcgccgc ccgagctcgc ggtgtcgtcc
     1321 ggactcgaac gcgaaggcga ccattaccgt ctcgaagcgc acgagatccc agtcgcgact
     1381 tacagcgcgt tcggaaagct cgcgcgcgat gtggtgccga tcgacgacgc gcgcgaagag
     1441 ctcgacgtga cggtgctgga cggagaactc gccgtgaacg agcagcgcat cacgcgctgg
     1501 gccgcggagc gagcgcgcgc cgtatccgcg ttttaccgcg gtttccccgc cgcgcgcgcg
     1561 tccctgttcg tggtgccagt ccccaaccga cgcgaagtgg tgttcggcaa tttgttgccg
     1621 gagagttcac cggccgcggt gctcgaggtc ggctcgctca cgaacgatgc cgcgctcgac
     1681 gacgactggg tgctcgttca cgagctcttc cacatcgggg tgccgtcttt tcacggcgaa
     1741 gggaagtggt tcgacgaggg tctcgcgacg tacttcgagc ccatcatccg agtgcgctcc
     1801 gggttgctcg agccgctgaa cgcttggcgc gagttcgcac tgacgatgcc gcgcgccgag
     1861 cgcgctctga cacgcgacgg cctcgagcag agtgacgaca tctactgggc cggcgcgatc
     1921 ttttgtttgc tcgccgacgt gaccgctcgc gagcaatccc aagggcgcca ggggctcgaa
     1981 gatgggcttc gcaaggtgcg cgccgcgggc ggcaacgcct ccgaggtgtg gccgctgaaa
     2041 aagacgctcg aaatcgccga ttctgcgttc gcccagccgg tgctggtgcc gctctggaag
     2101 cgctacgcgc gcaaaccggc gcccctcgac ctcgcggcgc tgttcgaggc gctcggcgtc
     2161 acgcgggacg gaagcggagt gcacttgtcg gacgcagcgc cgcgcgcgtg ggtgcgtcgc
     2221 tcgatcttcg agccgggcgc ggcgatcagc gcgagccgct gacgagccaa gcgcgggcgt
     2281 ccatgaagac gccgctttcg gtgacgaacg gcgcgaacac cttgcgcaga gtcgccgcga
     2341 ccgcgtcttt cgtcgcggcg tcggcgtcgc cgagcagacg cgcgcccgga ccgacttgaa
     2401 gcgagtaggc cacggcttgc tccagtgttt cggcgccgcc gaggtgcacc ttgagctcca
     2461 gcgctttgaa ctccaccgtc tggcactgcg cgagcatgag cgcctcgccg aggggctccg
     2521 gatccgcgaa acgaaacggg ccggggcgcc cgggagtcag aaaatccggc agggcctcca
     2581 tgcgcagctg ttcgcgaacg gcgctggccg gcagaaacgc ccaggggtta cgctcgatgg
     2641 actgccagca tacgaaagcg agccggccgc cgcgcttcag cgccgaaacg aggttcatac
     2701 aagcggccgc cgtgttctcg aagaacatga ccccgaaccg tgagaacacg gcgtcgtatc
     2761 gggcgggctc gaacgaatga ctctgcgcgt ccgcaagctc gacctcgaca ttggcgcgac
     2821 cgctcgccgc gagtcgctgc cgagcgcgct cgaccatgac ctccgaaacg tcgaccccga
     2881 gcaccttgcc gctcggcccg acgcgcccgg cgatttcgac cagcgtctga cccgtaccgc
     2941 aacccacgtc gatgaccgac tgacccgcgg cgagcgaaag gctttcgagc gcagcgcgac
     3001 cgaacggcac gagtgtcttg tcgatgcgcg cctcttcgcg cacccacgcc tggcccgaac
     3061 ggtcacgcca acagtccgag acggagcttc cttcggaagt gctcacgaga cacaggcgta
     3121 tcatgtgccg gccacaaact gggacgcttt tttcagctca gtaggtcgag tgcgactttg
     3181 attgtcaccg ccgctgcgac ggacagcacg accacgcgca ccgtgcggtc gccatgtcga
     3241 agcgccaaac gcgcgccgat gttagcgccc acccaattgg caacacccat cggcaacgcg
     3301 accggccacg cgacgctgcc tcgcagctga aacacgagca acgcagctat gttggagccg
     3361 aagtttatta ccttggcgtt gccgctcgca cgagttggag aatctccgaa aaaatgaacg
     3421 aaacccgcaa tcaacaatgt gcccgttccc gggccgaaga acccatcgta cgcgccgccc
     3481 gcgagcccga gcgccacaac gccccacttg ccgtccgagt tcggcgcgct cggacgtaac
     3541 aagtgctcgc gcaatatcat tactacggcg gcggcgccga gcagaacgag caccagcggc
     3601 cgaagcggcg cgggcgggat cagggatacg agctccgcac ccgcgagtga tccgatgaac
     3661 ccagaggcga gcccccacgg taccttcgag cgatcgagcc cgccgcgccg gaagtaggtc
     3721 gcgaacgagg cggtcgcgcc aaagctcgcc tgccccttgt tggtagcgag cacggcgtgc
     3781 ggcccgagcc cggccgcgag tagtgccggc accgtgacca aacctccgcc tccgccgatc
     3841 gcatcgacca aacccgcacc aaaagccgcg gcggctagca acgcgatggc ctgtactccg
     3901 agctccatct cgcctgcgtt gtacaccctt ggccaggcgc acggcc
//
LOCUS       DASTKS010000369         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_952165, whole genome shotgun sequence.
ACCESSION   DASTKS010000369 DASTKS010000000
VERSION     DASTKS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952165"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="metK"
                     /locus_tag="VFQ35_07610"
     CDS             complement(<1..868)
                     /gene="metK"
                     /locus_tag="VFQ35_07610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET9930536.1"
                     /translation="MSSYLFTSESVTEGHPDKICDAISDAVLDAALAGDKRSRVACET
                     LCKTGFVVVAGEITTKSSLDFGKIARGVIKEIGYTDSSMGFDYETCGVLTAIEPQSPD
                     ISQGVTEGEGLFKEQGAGDQGLMFGYACDETPELMPAPITYAHALARQLTAVRKAKKV
                     DFLRPDGKSQITLEYEDGKPKRIETVVVSTQHSPDVKHKQLKDAVMELVVKKSLPANL
                     LDKKTKYFINPTGRFVIGGPFGDAGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRS
                     GAYFGRFVARQVV"
     gene            complement(1083..1448)
                     /locus_tag="VFQ35_07615"
     CDS             complement(1083..1448)
                     /locus_tag="VFQ35_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9930537.1"
                     /translation="MRGDLRANVFALFVGLSAFACTPQRGTIGAVLGQHSDGTLVVRD
                     TPRGLAAERGGVLVGDEILLIEGRDVRGLRAEAVHQALAGEVGDPVKLTLIRGERVVR
                     LTLTRTPARSGKAAPSRVE"
     gene            complement(1457..2635)
                     /gene="argJ"
                     /locus_tag="VFQ35_07620"
     CDS             complement(1457..2635)
                     /gene="argJ"
                     /locus_tag="VFQ35_07620"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET9930538.1"
                     /translation="MVSVPGFRFSAVAAGIRKDGRIDTALAVADRPATVAGVFTRNLV
                     RAAPVVIASERVLSASAQAVLVNSGCANACTGAAGHAAAESSTAAVARALGIDANGVL
                     PASTGVIGVVLPAARIIEASESLVAGLSADKSDDFARAIMTTDRWPKVASTTLTRGGS
                     SATVLAIGKGAGMIHPDVGPPHATMLVFIFTDAQVSAEDLSAALVRATDDTFNSCTVD
                     GDTSTNDSVFAFASGVSALRAGREELTAALFQVCDELARSMVKDGEGSEHVVEIAVRG
                     LADKVAARNVARTVATSLLVKTAIYGRDANWGRLLAAAGRAGVPFDPNRAIVRIGDAE
                     IVRNGVSTGAEAEARASQILRTPEFRIELVLGDGPGEARYLTSDIGHAYVDVNASYRS
                     "
     gene            3158..4150
                     /locus_tag="VFQ35_07625"
     CDS             3158..4150
                     /locus_tag="VFQ35_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930539.1"
                     /translation="MPETKPRELQATSPSGGTGSSNGDSTPPEDSIEQVAKRRRFEQW
                     VASVRPKAPPPKPNANSARTSSSSSVSAAQLPTAPPPPDHADAAPDISVSSNPPPLMS
                     TSPPPAGHAISNPPPSFFPIAPQRESDSTQLYQMVPADLLRKARELQAASEPPAPRPA
                     AMPSADSLREDPWATADERTAVFAPPPELLASVRAAAASAGLIPKDEPAGETSPPSGR
                     ITAEFSLQDPDSASASASAPSLEAAPASASVLDAETAKVARSTGVEAAESPSSSPPPA
                     SFRRASPEEEAPARARWPWAVAVLVIAASLATLAVRSQKSPPPGRLPNVGAPAR"
BASE COUNT          680 a         1458 c         1409 g          706 t
ORIGIN      
        1 tcacgacctg tcgcgcgacg aagcggccga agtacgcgcc gctgcggtcg actttcgacg
       61 gatctttgcc gctgaacgcg ccgccaccgt gccggcccat gccgccgtag gtgtcgacga
      121 tgatcttgcg accggtgagg cccgcgtcac cgaacggacc gccgatgacg aagcggccgg
      181 tggggttgat gaagtacttg gtcttcttgt cgagcaggtt cgcggggagg ctcttcttca
      241 cgacgagctc catgaccgcg tctttgagct gcttgtgctt cacgtcgggc gagtgctgcg
      301 tcgaaacgac gaccgtctcg atgcgcttcg gcttgccgtc ttcgtactcg agcgtgatct
      361 ggctcttgcc gtcgggacgc aagaagtcga ccttcttcgc cttacgaacg gcggtgagct
      421 gacgcgcgag cgcgtgcgcg tacgtgatgg gagcgggcat gagctccggc gtctcgtcgc
      481 aagcgtaacc gaacatcaag ccctgatcgc cggcgccttg ttccttgaag agaccctcgc
      541 cctcggtcac gccctgcgag atgtcgggag actgtggctc gatcgcggtg agcacgccgc
      601 acgtctcgta gtcgaagccc atcgacgagt ccgtgtaacc aatctccttg atcacgccgc
      661 gagcaatctt cccgaaatcg agcgagctct tggtggtgat ctcacctgcc acgacgacga
      721 agccggtctt gcacaacgtt tcgcaagcga cgcggctgcg cttgtcgccc gcgagcgctg
      781 cgtcgagcac ggcgtccgag atcgcgtcgc agatcttgtc ggggtggccc tcggttacgg
      841 actcggacgt gaaaaggtag ctgctcatct ctgctccatc gacgggacct gaagggcccc
      901 aagaatgcgg taggcggggc gtagccccat ttcggcggcg gaagctacca cggatttcgg
      961 agttccgaat ctcgggcttt ggggcgccaa aataggcaag gttctcgcca ttcgagcgct
     1021 cccaggcggt ccccgcgccc caaattgccg gccgtttcgg gcccgcgccg cgcgtcctcc
     1081 ccctattcca cgcgcgaggg ggcggctttg ccgctccgcg cgggggtgcg cgtcaacgtg
     1141 aggcgcacga cccgctcgcc tcgtatcagg gtgagtttga cggggtcccc gacctcgccc
     1201 gcgagcgcct gatgtactgc ctcggcgcgc aggcctcgta cgtctcgtcc ctcgatgagt
     1261 aggatttcgt cgccgactag aacccctccg cgttccgcgg caagcccccg gggcgtatcg
     1321 cgaactacga gcgtcccgtc gctgtgctgg ccgaggacgg cgccgatcgt gccgcgctgg
     1381 ggcgtgcagg cgaacgcgga gagccctacg aagagcgcga agacgttcgc ccgaaggtca
     1441 cctctcacga gcctcgtcaa gaacggtagc tggcgttcac gtcgacgtag gcgtgcccga
     1501 tgtcgctcgt gaggtagcgc gcctcaccgg gcccgtcacc gagcacgagc tcgatgcgaa
     1561 actccggcgt acgtaggatt tgactggcgc gagcctcggc ttccgctccc gtcgagacgc
     1621 cgttccgaac gatctcggcg tctccgattc gcacgatcgc gcggttcggg tcgaaaggga
     1681 ccccggcgcg cccggcggcc gcgaggagac ggccccagtt cgcgtcgcgg ccgtagatgg
     1741 ccgttttcac gagcagcgac gtcgcgaccg ttcgcgcgac gttgcgagct gcgaccttgt
     1801 cggcgaggcc gcgcaccgcg atctccacca cgtgctcgga gccttcgcca tccttgacca
     1861 tggaacgggc gagttcgtcg cacacttgga agagcgcggc ggtcagctcc tcgcgacctg
     1921 cgcgaagcgc ggaaacgccc gacgcgaacg cgaacacgct gtcgttggtg ctcgtatcgc
     1981 cgtcgaccgt gcacgagttg aaggtgtcgt cggttgctcg aaccagcgcg gccgaaaggt
     2041 cctcggcgga gacctgcgcg tcggtgaaga tgaacaccag catggtggcg tggggcgggc
     2101 cgacgtctgg atgaatcatg cccgcgccct tgccgatggc gaggacggtc gcactgcttc
     2161 ctccacgcgt cagcgtcgtg ctcgcgacct tgggccagcg atccgtcgtc atgatggcgc
     2221 gcgcgaagtc gtcgctcttg tcggcagaga ggccagcgac gagagactcg ctcgcttcga
     2281 tgatgcgcgc ggccggcaaa acgaccccga tgactccggt ggaggcgggc agcacgccgt
     2341 ttgcgtcgat ccccaacgcg cgcgcgaccg cagccgtcga gctttcggcg gcggcgtgtc
     2401 cggccgcgcc cgtgcaagcg ttggcgcaac cgctgttcac gagcaccgct tgcgcgctcg
     2461 ccgagaggac tcgctcgctg gcgatgacga ccggcgcagc acgcaccagg ttgcgcgtga
     2521 agactcccgc gacggtggcg gggcggtcgg cgaccgcgag cgccgtgtcg attcgaccat
     2581 ctttgcggat tcccgccgct acggcggaga aacgaaaacc aggaacgctg accattgggg
     2641 cgcgcgagct tacgacagcc gagcccacct ggcgaggcaa acgtgcgcgc gcgggcggcg
     2701 cggtttcagt gctgcctcgc gcgaccgagg cacaatggcg ctcggcgcgc gcccgagctt
     2761 cgggccattc gcagtgcaac gtcgcgcgag tgcgccgacg cggcgcgcgc ggcagcaatt
     2821 gctgtcgggc actcgtcgcg acagtgcgaa aaagtcgtac cccggccctc cacggcgaca
     2881 gctggcgccc tcattttcaa cctgattccc gctgttcggt gcgaagggtc agcacccctc
     2941 gcacccaggt attggtgcgc ctttcggggt tttgacgcct tgaaagtccc cggtcgagag
     3001 atgtgacgaa cgatgaggtg ctgtgcgctc gaccgagcgc accttggggg tggttgcgcg
     3061 cgacggcgct cttgggcaaa gcggcaattg cattgcgctg caacccaggt agctttcttg
     3121 agatgggctc gcgcacgtgg gcccgagagc tcgcccaatg cctgagacga aacctcgcga
     3181 acttcaagcg acctcgccgt cgggcgggac gggttcgtcg aatggcgaca gtactccgcc
     3241 agaagacagc atcgagcagg tcgccaagcg gcggcgcttc gagcaatggg ttgccagcgt
     3301 gaggccaaaa gctcccccac caaaaccgaa cgcgaactct gcccgcacct cgagctcatc
     3361 gagcgtgagt gcggcgcaac ttcccaccgc accgccgcct ccagatcacg ccgacgcggc
     3421 tcccgatata tccgtcagca gtaacccccc accgctcatg tccaccagcc caccaccagc
     3481 aggtcacgcc atttcgaatc cgccaccctc gttctttccg atcgctccgc agcgcgagtc
     3541 ggacagcacg cagctctacc aaatggttcc ggcggatctg ttacggaagg ctcgagagct
     3601 gcaggctgcg agcgagcccc ccgcgccccg cccagcggca atgccgagcg ccgattcgct
     3661 gcgcgaggat ccttgggcga ccgcggacga gcgcacggcg gtgttcgctc cgccgccgga
     3721 gttgctcgct tccgtccgcg cggcggccgc cagcgccggt ctcattccca aagacgagcc
     3781 cgcgggagag acatcgccgc caagtggtcg catcacggcc gagttcagtc tgcaagaccc
     3841 ggactcggcc tccgcgtccg cgtccgcgcc gagcctcgaa gcggcgcccg cctccgcttc
     3901 cgtgctcgac gcagagacgg cgaaggtggc tcggtcgacc ggcgtcgaag ccgcggagtc
     3961 tccgagttcg tctccgccgc cggcgtcatt ccgccgggcg agtccagagg aggaagcgcc
     4021 tgcgcgggca cgctggccgt gggcggtcgc ggtactggtc atcgcagctt cgcttgcaac
     4081 gcttgcagtt cgttcccaaa agtctccgcc accagggcgg cttccgaacg tcggcgctcc
     4141 tgcgcgctga cgccatcgca cgaacgtcgg caccgcgggg ccacgctgac ctgcgcgaac
     4201 cgccgcggtc gcactgcgct agcctgggac tcgcgctcag ggcgacgtag cgc
//
LOCUS       DASTKS010000370         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_282425, whole genome shotgun sequence.
ACCESSION   DASTKS010000370 DASTKS010000000
VERSION     DASTKS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282425"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2447)
                     /gene="ppdK"
                     /locus_tag="VFQ35_07630"
     CDS             complement(<1..2447)
                     /gene="ppdK"
                     /locus_tag="VFQ35_07630"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9930540.1"
                     /translation="MTRKSIYRFGFGSAEGRAEWKDLLGGKGANLAEMANMGIPVPPG
                     FTITTEVCVDYLRLSVLPEGLAQAVREGLEWLERGLGKRFGGTENPLLLSVRSGARSS
                     MPGMMDTVLNLGLNDLTVRVLAKQSQSERFALDAYRRLLCMYADVVLGIERSLFDPPL
                     DAARRSVARQRGLEIPSDVEVLARLVPDPVLGAAELSQVVHDYKQIIFEQTGREFPAD
                     PYEQLTQAITAVFESWNNPRARLYRNMHEIPHDWGTAVNVQAMVFGNLGDTSATGVAF
                     TRNPSSGERRFFGEWLPNAQGEDVVAGIRTPLPLSKNNESQGNSLEELLPESYRELVQ
                     VQEKLEQRFRDMQDLEFTIEAGKLYLLQTRNGKRSARAAVRIAVDMVDEGLLHPDEAI
                     LRVDPERLQELLFPAIDPKTSAQPVARGIAASPGAVSGRAVFSADDAEAYAARDENVI
                     LVRIETSPEDLHGMKAARGILTARGGATSHAAVVARGMGRPCVSGCSALHVDPAAGRM
                     TIHGADGSVVATIHHGEAITIDGSSGQVYCGVIETIDAELGPEMGTLLKWTERVRRLR
                     VRANADTPAQAEKALSFGAEGIGLCRTEHMFFNEERIAAVREMILARDTAARERALAK
                     LLPFQTHDFTELFRVMKDRPVNIRLLDPPLHEFLPQEHTHIAELAATMDVSVADVKRK
                     IAELYEFNPMLGHRGVRLGITFPEIPAMQVRAILDAACTVKKEGHDVRPEIMIPLAFS
                     AAELKAMRRIVDEVAREVFARQGVEVKFKFGTMIELPRAALLADQLATEAEFFSFGTN
                     DLTQTTLGVSRDDAGSF"
     gene            complement(2650..3099)
                     /gene="cynS"
                     /locus_tag="VFQ35_07635"
     CDS             complement(2650..3099)
                     /gene="cynS"
                     /locus_tag="VFQ35_07635"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HET9930541.1"
                     /translation="MDKIEAKRLVLAAKKKKKLTWAALAESVGMSEMWVASCGYGENS
                     MTPESAAKLCAELELDTEVRDALTEFPTKGNSLDGKIVPTDPLMYRFYEILCVYGTAL
                     KDVIQEKCGDGIMSAIDFTIDVRKESNPKGDRVVVTMDGKFLPYRRF"
BASE COUNT          561 a         1092 c         1003 g          620 t
ORIGIN      
        1 aagaagctgc ccgcgtcatc gcgcgacacg ccgagcgtcg tttgggtgag atcgttcgtt
       61 ccaaacgaaa agaactcggc ttcggtcgcc agttggtcgg cgagcagggc tgcgcgcggc
      121 agctcgatca tcgtgccaaa cttgaacttc acctccacgc cctgccgcgc gaacacctcg
      181 cgcgccactt cgtccacgat gcggcgcatg gccttgagct ccgccgcgct gaaggcgagc
      241 ggaatcatga tctccggtcg gacgtcgtgg ccttccttct ttacggtgca cgccgcatcc
      301 agaatcgcgc gcacctgcat cgcggggatc tccgggaagg tgatacccag ccgaacgccg
      361 cggtgaccga gcatggggtt gaattcgtag agctccgcga tcttgcgctt cacgtcggcg
      421 acgctcacgt ccatcgtcgc cgcgagctcg gcgatgtgag tgtgctcctg cggcaagaac
      481 tcgtggagcg gcggatccag caggcggatg ttcaccggcc ggtccttcat gacgcggaag
      541 agctcggtga agtcgtgggt ttggaacgga agcagcttgg cgagagctcg ctcgcgagcg
      601 gcggtatcac gcgccaggat catctcccgc acggcagcga tacgctcttc gttgaagaac
      661 atgtgctcgg tgcgacagag cccgatgccc tccgccccga aactcagcgc cttctcggcc
      721 tgtgccggcg tgtcggcgtt cgcgcgcacc cgcaagcggc gcactcgctc ggtccacttc
      781 agcagggtgc ccatttcggg gccgagctcc gcgtcgatcg tctcgatgac gccgcaatag
      841 acctgcccgc tcgaaccatc gatggtgatc gcttcaccgt gatggatggt agcgaccacg
      901 gacccgtccg cgccgtggat cgtcatcctg cccgcggccg gatcgacgtg cagggccgag
      961 cagcccgaaa cgcagggccg tcccatgccg cgcgccacca ccgccgcgtg gctcgttgcg
     1021 ccaccgcgcg cggtgagaat gccacgcgcc gccttcatac cgtggagatc ttcgggcgag
     1081 gtctcgatgc gcaccaagat cacgttttcg tcgcgcgcag cgtatgcctc cgcgtcgtcg
     1141 gcgctgaaca cggcgcgccc ggacacggcg cccggtgacg ccgcgatgcc gcgtgcgacc
     1201 ggttgcgcgc tcgtcttcgg atcgatggcc gggaacaaaa gctcttggag gcgctccgga
     1261 tcgacgcgga gaatcgcctc gtccggatgc agcaagccct cgtccaccat gtcgacggcg
     1321 atgcgcacgg cggctcgtgc cgagcgctta ccattgcgcg tctgcagcaa gtagagcttg
     1381 cccgcctcga tcgtgaactc cagatcttgc atgtcgcgga agcgctgctc gagtttctcc
     1441 tgaacttgaa cgagctcgcg atagctttct gggaggagct cctcgagtga gttcccctga
     1501 ctctcgttgt tcttcgaaag aggcagcggc gtacgaatcc cggcgaccac gtcctcaccc
     1561 tgcgcgttcg gcaaccattc tccgaagaat cggcgctcgc cgctgctcgg gttgcgcgta
     1621 aaagcgacgc cggtcgcgct cgtgtcaccc aggttgccga agaccatggc ctggacgttc
     1681 acggctgtgc cccaatcatg cggaatttcg tgcatgttgc ggtagagccg cgcgcgcggg
     1741 ttgttccagc tctcgaacac cgccgtgatc gcctgagtca gctgctcgta gggatcagcc
     1801 gggaactcgc gccccgtttg ctcgaagatg atctgcttgt agtcgtgcac gacctgagaa
     1861 agctcggccg cgcccaagac cgggtcgggg accaatcgcg cgagcacttc gacgtcgctc
     1921 gggatctcga gtccacgctg gcgggcgacg ctgcgccgcg ccgcgtccag gggtggatcg
     1981 aacaggcttc gctcgatgcc gagcaccacg tccgcgtaca tacagaggag ccgacgatac
     2041 gcatctagag cgaagcgctc ggattggctc tgcttcgcga gcacgcgaac cgtgagatcg
     2101 ttcagcccca agttgagaac cgtatccatc atgccgggca tcgagctccg cgcaccggaa
     2161 cgcacactca agagcagcgg gttctcggtc ccaccgaagc gcttgcccag ccctcgctcg
     2221 agccactcca gcccttcacg caccgcttgc gcgagccctt cgggaagaac cgacagacga
     2281 agatagtcga cacaaacctc ggtcgtgatg gtgaaccccg gcggtacggg gatccccatg
     2341 tttgccatct cagcgagatt ggcacccttg cccccaagca agtccttcca ctcggcgcgt
     2401 ccttctgcgc ttccaaatcc gaagcgataa attgacttac gtgtcatgcg ccgctcatcc
     2461 gcaagcttcg tgccgagcgc gcacgacgcg acgagctgcc gagttgcgcg ggtttttcgt
     2521 gtgattcgcg tcgccgggcg gctacaagca gcgcacgaat tgcgtcgatt tcgaaagagc
     2581 cgcgccgcgt gcgcctcgcg ccctctcagc gcacgccgac gacgattctg ctcacccgat
     2641 gggcggtcac tagaagcgtc ggtatggcaa gaacttgcca tccatcgtga ccacgacgcg
     2701 gtcgcctttg gggttcgact ctttacgaac gtcgatcgta aagtcgattg cactcatgat
     2761 gccgtctccg cacttctctt ggatgacatc cttcagcgcc gtcccataaa cgcataggat
     2821 ctcgtaaaag cggtacatca gcgggtcggt ggggactatc ttgccgtcga ggctgttgcc
     2881 cttcgtcggg aactcggtga gcgcatcccg cacttccgta tccagctcga gctcggcgca
     2941 gagcttggct gcagactctg gtgtcatcga gttctcgcca tagccgcacg aagcgaccca
     3001 catctctgac ataccgacgg actcggccag cgccgcccag gtgagcttct tcttcttctt
     3061 ggccgcgagt acgagccgct tcgcttcgat cttgtccatg ttcacgcctc cgtcaccggc
     3121 accgttcttc tgcccgcgtt gcgggagacg gtcggtattt tgtcaggcgt gtttccggca
     3181 cctcgaaagc gcggcgctgc gctctcgtcc gttcagggcc gtgtttgtac gagccggtcg
     3241 cgagcaagcc gagagcgcag caaggcgcgc atcgag
//
LOCUS       DASTKS010000371         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_282428, whole genome shotgun sequence.
ACCESSION   DASTKS010000371 DASTKS010000000
VERSION     DASTKS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282428"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFQ35_07640"
     CDS             complement(<1..375)
                     /locus_tag="VFQ35_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930542.1"
                     /translation="MSRARATALALVNWKGVFFERYLLDRHVTALEGANGAGKTTVMI
                     AAYVVLLPDMTRLRFSNVGESAGSGDRGIWGRLGESARPSYSVMEFEFGDGQRVLAGV
                     QLARKAEPSVELVPFLVTGADAS"
     gene            complement(372..1433)
                     /locus_tag="VFQ35_07645"
     CDS             complement(372..1433)
                     /locus_tag="VFQ35_07645"
                     /inference="COORDINATES: protein motif:HMM:NF016198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HET9930543.1"
                     /translation="MSESAFTRLEDVILDESFPDVDLALRRGRHIDAEDGAWFTFLCD
                     AEAHLEAFYRRFGADLVRRSEGYFYLLPTSERLGRRQLSVAEMLVGQALTLLYLDPST
                     IQGGGTVTKADVLSHLASVMGTESLVRALNPAKKKKLDERVAEETVRSRVGEALRRLG
                     TLGFVDFVEEDRMRLRASLMRFAEPVRGGTADTEALERLVAAGEVVFTDEEEEASESK
                     VSDSEDSEEPTEEPFLLSDAELAELDPSDESITRSDTEDTEEPALVPNDLAVPDPVDR
                     DPSRQSQPQFTPSPSDEHEPEPILEPEPVHEPEPIHEPEPIHEPEPIHEPEPVVSADP
                     SFEPAPDSSSESESPGEEP"
     gene            complement(1430..2743)
                     /locus_tag="VFQ35_07650"
     CDS             complement(1430..2743)
                     /locus_tag="VFQ35_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensin subunit MukF"
                     /protein_id="HET9930544.1"
                     /translation="MPSTPEDPNRALAALAERGVSLSLTSLDVCFAAALYLRANGEAL
                     TSFSEAELFAVFDDVVGIVEPGSDTARRRATPAIQRLREQRLLARVDGAGVVRSGEYA
                     LTRLATGIVEFYLHDETLTRESLTLLTRTLLASLGSIRDAASRAQTTEAWRAEVSAPL
                     SVTVGDLVSGIERRQRGLDAQQEEFQREITALLQADWFGAVERCQALLEASAATLREL
                     NEILMRDTHELSSALQEIQELAQLSSALEAEEAARRVADQIERIAAWGASRQRAWSEY
                     YRYVHHFLRDVVRLDPTRALTERLREQLSGRRGRRFALSVANAPPVRVLRELKPVVAP
                     PPVRRPRAEREKPTSEETPHDPDAELEARIVRELDDGAADLSSLTARITAEFPKESRF
                     VMAGRIAQIVARLTRPIAARERPWVASGDDFELEEWAVRSARSEG"
     gene            complement(2778..4310)
                     /locus_tag="VFQ35_07655"
     CDS             complement(2778..4310)
                     /locus_tag="VFQ35_07655"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET9930545.1"
                     /translation="MTVAGGKPRPAVLFGMVLAIATSGLVYELGMAAVGSYLLGDTVH
                     QFSLVIGVYLSALGIGAYLSRFVGKRLGIVFIDVELATALVGGLSAEVLLATFGWIGA
                     WQPFFYGTVLLVGILVGLELPLLIRVLEHRLELRELIAKALTFDYVGSLLGSLAFSLL
                     LLPRLGLARASLVSGLINAFVGLASTYAFAGLEGTAEGSLSRARLRAFAVLVLLGLGF
                     LQSERALELSDRTMHSGTVVHAEQSRYQRIVVTEAAGDVRLFLNGHLQFSSRDEARYH
                     ESLVHPALTLARRRARIMIGGGGDGLAAREVLRWPDVERLTLVDLDPRMTELFSADAR
                     LVAINEGSLRNRRVHVENDDALSYLRRGKDVFDVVILDFPDPTNYAVGKLYSVEFYRA
                     LAARLAPEGIAVVQATSPLFARSAFWCIERTLAEAGFRTLPYHVLVPSFGEWGFVLAR
                     RGDLAAPDDVPIRDLRYLDRRTLAGLFVFPPDSAPLSVRVNRIDNQALVGYYDADWER
                     WN"
     gene            complement(4323..4595)
                     /locus_tag="VFQ35_07660"
     CDS             complement(4323..4595)
                     /locus_tag="VFQ35_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET9930546.1"
                     /translation="MEARFVVSARNVEGRSMSAEISPFVHTVVASLVYSLIGLLLFGL
                     CFAVISKLAPFSIRKEIEEDQNTALAILIGSVILGLSWIIAAAIHG"
     gene            4756..5568
                     /locus_tag="VFQ35_07665"
     CDS             4756..5568
                     /locus_tag="VFQ35_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930547.1"
                     /translation="MFVLLGPDFEGTPQAYQRLARATGLVAYDLRARVRNGAWGVVKA
                     LADPGQAAALVQALADAEFRPVSIERTVAHDSARPIVPVRSLSLGEGEFVLGLRERDM
                     SVEYAALACVVRGEVLPGRSVQRASGNGPSSASFRAVAAVPETTVPINVGGSTFEAFQ
                     AADLHFLTAPWVARIDLHSLGGNASEASPRALDSLADELARRAGARVDRNVRTSSVAS
                     LAEQATPRRGGSEPPNVREARREQADERFDAYSRIIGEAERQARFRARRDAR"
     gene            complement(5573..>5787)
                     /locus_tag="VFQ35_07670"
     CDS             complement(5573..>5787)
                     /locus_tag="VFQ35_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930548.1"
                     /translation="PGRGRSLKRIALTLYVKQCEASRPAACARLAEMYEVGEVVQKSD
                     KSVQGLRARVVELCKTRPNELGCAGS"
BASE COUNT         1016 a         1876 c         1963 g          932 t
ORIGIN      
        1 cgaggcgtcg gcgcccgtca ccaaaaaggg caccagttcg acgctcggct ccgctttccg
       61 cgcgagttgc acgcccgcga gcacgcgctg cccgtcgccg aactcgaact ccatgacact
      121 gtacgagggg cgcgcggatt ctcccagccg tccccagatc ccgcggtcac ccgagcccgc
      181 tgattcgccc acgttgctga agcgcaggcg cgtcatgtcg ggcaagagca cgacgtacgc
      241 tgcgatcatg acggtggtct tgcccgcgcc gttggcgcct tcgagcgcgg tgacgtgtcg
      301 gtcgagcaaa tagcgctcga agaagacgcc cttccaattc accagggcga gcgcggtggc
      361 tcgcgcgcga ctcaaggttc ctctcccgga ctttcgctct cggacgagga gtctggcgcg
      421 ggctcgaagc tcggatctgc actgacgacc ggctcgggct cgtggatggg ctcgggctcg
      481 tggatgggct cgggctcgtg gatgggctcg ggctcgtgga cgggctcggg ctcgaggatg
      541 ggctcgggct cgtgctcgtc gcttggcgac ggagtgaatt ggggctgcga ttggcggctt
      601 gggtcgcggt cgaccggatc cgggaccgcg agatcgttcg gcacgagagc gggctcttcg
      661 gtatcttccg tgtcggagcg cgtgatgctt tcgtcgctcg ggtcgagttc ggcgagttca
      721 gcgtcgctga gcagaaaggg ctcttcagtg ggctcctcag aatcttcgct gtccgatacc
      781 ttggactccg atgcttcttc ctcttcgtcg gtgaagacga cttcgcccgc cgcgacgagt
      841 cgctcgagcg cttccgtgtc ggccgttccg ccgcgcacgg gttcggcgaa gcgcatgagc
      901 gacgcacgca gccgcatgcg atcttcttcg acgaagtcta cgaagccgag cgtcccgaga
      961 cgacggagtg cctcgccgac gcgcgagcgc accgtttctt ccgcgacgcg ctcgtcgagc
     1021 ttcttctttt tcgccggatt caacgcgcgc acgagcgact cggtgcccat cacgctcgcg
     1081 aggtgcgaga gcacgtcggc cttggtcacc gtgccgccgc cttgaatcgt cgacgggtcc
     1141 aagtagagca gcgtcagcgc ctgaccgacc agcatctctg cgacgctgag ctgacggcgt
     1201 ccgaggcgtt cgctcgtcgg caaaaggtag aagtagccct cgctgcggcg gaccagatcc
     1261 gccccgaacc ggcgatagaa ggcctcgagg tgcgcctccg cgtcacacag aaaggtgaac
     1321 caagcgccgt cctccgcgtc gatgtgacgt ccgcggcgca gcgcgaggtc gacgtcgggg
     1381 aacgactcgt cgagtatgac gtcttcgaga cgggtgaacg cgctctcgct catccttcgc
     1441 tccgagcgct tcggaccgcc cactcttcga gctcgaagtc gtctcccgag gccacccagg
     1501 ggcgctcccg cgcggcgatg ggtcgggtca accgagccac gatttgcgcg atgcgaccgg
     1561 ccatcacgaa gcggctctct ttggggaatt cggctgtgat gcgcgcggtg agcgagctca
     1621 aatctgccgc tccgtcgtcg agctctcgaa cgatgcgcgc ttcgagttcg gcgtcgggat
     1681 catgcggggt ctcctcgctg gttggcttct cgcgctcggc gcgcgggcgt cgcactggag
     1741 gcggggccac gaccggcttg agctcgcgca ggacgcgcac gggaggcgcg ttggcgacgc
     1801 tgagcgcgaa gcgccggccg cggcgcccgc tcaattgctc gcgcagccgc tccgtcaggg
     1861 cccgcgtcgg gtcgagtcga acgacgtcgc gcaggaaatg gtgcacgtaa cggtagtact
     1921 ccgaccaagc gcgctgacgc gaagcgcccc acgcggcgat gcgctcgatt tggtcggcga
     1981 cacggcgcgc ggcttcttcc gcctcgagcg cgctcgagag ctgcgcgagc tcttggattt
     2041 cctggagcgc cgaagaaagc tcgtgcgtgt cgcgcatcaa aatttcgttc agctcgcgga
     2101 gggtcgccgc gctcgcctcg agcagcgctt ggcaacgctc caccgcgccg aaccaatcgg
     2161 cttgcaagag agccgtgatt tcgcgctgaa attcctcttg ctgggcgtcg agaccgcgtt
     2221 gacgccgctc gatgccgctc accagatcgc ctaccgtcac cgagagcggc gcgctgacct
     2281 ctgctcgcca tgcctcggta gtctgggctc gactcgccgc gtcgcgaatg ctcccgaggc
     2341 tcgcgagcag ggtgcgcgtg agcaacgtga ggctctcgcg ggtcaaggtc tcgtcgtgca
     2401 aatagaactc gacgatgccg gtcgcgaggc gtgtgagggc gtactcgccg ctgcgcacga
     2461 cccccgctcc gtccacccgt gcgagcaatc gctgctctcg caagcgctgg atcgccgggg
     2521 tggcgcgccg tcgggcggta tcgctgccgg gctcgacgat gccgactacg tcgtcgaaga
     2581 cggcgaagag ctcggcctcg ctgaaggagg tcagcgcctc gccgttcgcg cgcaggtaga
     2641 gcgccgccgc aaagcacacg tcgagcgagg tgagcgaaag agaaacaccg cgttcggcga
     2701 gcgcggcgag cgcgcggttg gggtcctcgg gcgtacttgg caagggcgag ctcttcggaa
     2761 cggcgggccc gccgggatca gttccagcgt tcccagtcgg cgtcgtagta gccgacgagc
     2821 gcctggttgt cgatgcgatt gaccctgacc gatagaggcg cgctgtccgg cggaaatacg
     2881 aacaaacccg cgagggtacg ccggtctagg tagcgcaggt cgcgaatcgg aacgtcgtcg
     2941 ggcgcggcga gatcgccgcg gcgcgcgagc acgaaccccc actcaccgaa ggacggcacg
     3001 agcacgtggt acggcagagt tcgaaacccg gcttcggcca aggtccgctc gatgcaccaa
     3061 aatgccgagc gcgcgaagag cggggacgtc gcctgaacca ccgcgatacc ctcgggggcg
     3121 aggcgcgccg cgagtgcgcg ataaaactcc accgaataga gcttgcccac cgcgtagttc
     3181 gtcggatcgg gaaagtccaa aatgacgacg tcgaagacgt ctttcccacg ccgcaagtag
     3241 ctgagcgcgt cgtcgttctc gacgtgaacg cggcggttac gcagcgaccc ttcgttgatg
     3301 gcgacgaggc gcgcgtcggc cgagaagagc tcggtcatgc gcggatccag atcgacgagc
     3361 gtgagccgtt cgacatcggg ccagcgcagc acttcgcgcg cggcgagtcc gtctccgccg
     3421 cccccgatca tgattcgcgc acggcgacgt gcgagcgtga gcgctggatg aaccaacgac
     3481 tcgtgatagc gagcttcgtc gcgggacgaa aactgcaagt gtccattcaa gaacagccgc
     3541 acgtcacccg ctgcttcggt cacgacgatg cgctgatagc gagactgttc cgcatgcacg
     3601 acggtacccg agtgcatggt gcgatcggag agctccagcg ctcgttcgct ttgcagaaac
     3661 ccgaggccga gcaggacgag cacggcgaac gctcgcagac gcgcgcgcga cagcgagcct
     3721 tcggccgtcc cttcgaggcc cgcgaacgcg taggtcgacg cgaggccgac gaatgcgttg
     3781 atgagccccg agaccaaaga agcgcgcgcg aggccgaggc gcggcaacaa gagcagcgag
     3841 aaggcgagcg agccgagcag cgagccgacg tagtcgaaag tgagcgcttt ggcgatgagc
     3901 tcgcgtagct ccaggcggtg ttcgagcacg cggatcaaaa gcggaagctc gagcccgacc
     3961 aggataccta cgagcaggac cgtgccgtag aagaacggct gccacgcgcc gatccagccg
     4021 aacgtggcga gcagcacctc ggcgctcagg cccccgacga gcgcggtcgc tagctcgacg
     4081 tcgatgaaca cgatacccag gcgttttccg acgaagcgcg acagatacgc gccaattccc
     4141 agggcagaga ggtacacccc gatgaccagc gagaactggt ggaccgtgtc ccccaagagg
     4201 tagctgccga ccgccgccat gccgagctcg taaacgaggc cggaggtggc gatagccaac
     4261 accatgccga acagcacagc gggccgcggc ttaccgccgg cgacggtcat cgctgggctc
     4321 ggtcagccgt gaattgcggc ggcgatgatc cagctgagcc cgaggatcac cgagccgatg
     4381 aggatggcga gcgcggtatt ttggtcttcc tcgatttcct tgcggatcga gaacggggca
     4441 agcttcgaga tcaccgcgaa acagagccca aacaaaagaa ggcctatgag cgaataaacg
     4501 agagaagcga ccactgtgtg gacgaaaggc gatatctcgg cgctcatgct gcgaccctcc
     4561 acattccggg cagaaacgac aaaacgagcc tccatcccgt agcatggtgg cccgaaaaat
     4621 gcctcgcccg aatgcgacgg ttcacgaaac ccttatagaa gcgagggccc gcgtgaactt
     4681 cgtttccttg gcgaagcaag aactcgtcgg taccgaagcg gcgttcgatt tgatagcgta
     4741 aaacgttgga gcgatatgtt cgtactgctt ggccccgact tcgagggaac gccgcaagcg
     4801 tatcagcgcc tcgctcgggc aaccggtctg gttgcctacg atttgcgggc gcgcgttcgt
     4861 aacggggcgt ggggcgtcgt caaggcgctc gcggatcccg gtcaggcggc ggcgctcgtc
     4921 caagcgctgg ccgacgccga gtttcgtccc gtatcgatcg agcggacggt ggcccacgac
     4981 agcgcgcgcc cgatcgtccc cgtgcgcagt ctgtcactcg gagaaggcga gttcgtgctc
     5041 gggttgcgag agcgagacat gtcggtcgag tacgccgcac tcgcctgcgt ggtgcgcggg
     5101 gaagtgttgc caggaaggag cgtgcaacgt gcgagcggca acgggccgag cagcgcaagc
     5161 ttccgagccg tcgccgcggt tcctgaaacg acagtgccga tcaacgtggg cgggtcgacg
     5221 ttcgaagctt tccaggccgc tgacttgcac ttcctcaccg caccttgggt ggcgcgcatc
     5281 gacctgcact cgctcggggg caacgcgagc gaggcaagcc cccgcgccct cgacagtctg
     5341 gcggatgagc tggcacgccg ggctggagct cgcgtcgatc gcaacgttcg cacctcgagc
     5401 gtcgcttcgc tcgcggagca agccactcca cgacgcggcg gcagcgagcc gccgaacgta
     5461 cgcgaggcgc gtcgcgagca ggccgacgag cgcttcgacg cctactcgcg catcatcggc
     5521 gaggccgagc gccaggcgcg cttccgagcc cgtcgggacg cgcgctagtt ctctacgagc
     5581 cggcgcagcc gagctcgttt ggtctcgttt tacagagctc gacgactcga gctcgcaggc
     5641 cctggacgct cttgtcgctc ttctgcacta cctcgccgac ctcgtacatc tcggccaggc
     5701 gcgcgcaggc ggcaggccgc gacgcttcgc attgtttgac gtacaaggtg agggcgatgc
     5761 gcttcagcga gcgtccgcgg cccggat
//
LOCUS       DASTKS010000372         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_729097, whole genome shotgun sequence.
ACCESSION   DASTKS010000372 DASTKS010000000
VERSION     DASTKS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729097"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..964
                     /locus_tag="VFQ35_07675"
     CDS             278..964
                     /locus_tag="VFQ35_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930549.1"
                     /translation="MRALVLLPLMLVACGSSATPPSKPPGLPPTPTTVTLQHPGGDAA
                     DPHQAALERLLSEPWGQQGDKDGQLIVALPDSGNWKRVRYWGVEHFLGFRYGKEHHAM
                     VVIFSQEVEEEAPSSEDCLRHFDAWGRPQIQSFDVQFDPFRPHHARFKDKAMISLAVD
                     GKLSLGFSRPQFSAAWAAYSLYPKACLISAVAVPWRTTPELAQKVRDRFVNEGFTQLQ
                     SLTEVRPVRK"
     gene            complement(1236..1472)
                     /locus_tag="VFQ35_07680"
     CDS             complement(1236..1472)
                     /locus_tag="VFQ35_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930550.1"
                     /translation="MDLFGMLVLSAALGALVTAHVVLVAGLATQPPRWRALAAFFLPP
                     LAPYWGFEARRYVWSSVWLFAAVSYVASAIAAAF"
     gene            1580..3478
                     /locus_tag="VFQ35_07685"
     CDS             1580..3478
                     /locus_tag="VFQ35_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HET9930551.1"
                     /translation="MRFSSLLVALSLGGLLLPLPLGLRRAHADGLTSRADIMPVKDIK
                     PGMKGYGLTVFSGTTPERFDVEVIDVLTNFRPRQDLILIKTKHPRLEVAKVVAGMSGS
                     PIYINNKMIGAYAYGWTFGAEPVAGVTPIRNMLDDLVRPLPPDIYGWRLATLPKPRAT
                     SSGKLGHNEATPDERLAFGGSRFRGPSYDLRAHASELASRGVQVPAAQSALASVSTPL
                     LLSGMTGGGMNLARDLLQPLGLEPLQAGGAGAPDPNAPTKFVDGGAIGVQLVRGDMSA
                     MGLGTVTRVEGDRLVAFGHPMMESGVTALPTAIGKVLWFLASDQRSFKIGMPVRELGT
                     LVDDRQASIVVSQSLKAPVIPMSVKIRGVPGLASSNWNFEVAHERFLSPSFVSVAWGS
                     ALQSVAAEHQDVTWTAKSVLKIKDYGEITLDDYGVAIGGTPDTGELSRSNLVRAVGGI
                     MNNPWQPVIIESINTELTLTFAREMLRLRGVDLVDPEVDAGQPVRLKLTLAPYSGPEI
                     TRVLSVDVPKTLAGQNVNIEVLPGYMEDREGAPPDNLSELIKNLENPTYPPRSVVVAF
                     SAGNAAVSFKGRVAKNLPPGALDALRPTASSVGPDAFQTVVRKVFPLSEFMVGRDKLS
                     VTVRPVLR"
     gene            3491..>3929
                     /locus_tag="VFQ35_07690"
     CDS             3491..>3929
                     /locus_tag="VFQ35_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930552.1"
                     /translation="MTFRRISRFFSGLGAVDDRRFAMQAKLSNYGLFRGIGALSVALG
                     ALVCASRADAVGTRHFLLDKGADFKGGDLKGVAVDSAGGVHAGLTLTAAPIQNAATIW
                     AALPQRDGSVLLGTGNEGKLMRLAQGASSVVGETKGLAVTSLVE"
BASE COUNT          654 a         1218 c         1383 g          674 t
ORIGIN      
        1 gccgtcgatg cagggcggtg gatgcagggc ggtggatgca gggcggtgga tgcagggcgg
       61 tggatgcagg gcggtggatg cagggcggct ggtgcagggc ggctggtgca gggcggctcg
      121 ggaaagaggg catgtaggcc caggggtgtt cgccgcgcac ttcgcacttc ggtttgggac
      181 caaaggttcg ggaattcggg tctttacggt ggcgcgccgc gtttttcggg ttcgggtgcc
      241 atactttcgc gggtcgctcc gtataacggt tcgtaagatg cgcgcgctcg tcctgcttcc
      301 gctgatgctc gtcgcgtgcg gcagctccgc cacaccgccg agtaagcctc cgggtcttcc
      361 gccgactcca acgacggtga cgctgcagca tcctggcggc gacgccgcgg atccgcacca
      421 ggcggcgctc gagcgcttgc tgtcagagcc atggggacag caaggcgaca aggacggcca
      481 gctcatcgtc gcgctacccg attcgggaaa ctggaagcgc gttcgctact ggggcgtcga
      541 gcactttttg ggctttcggt acggtaagga gcaccacgcg atggtggtga tcttctcgca
      601 agaggtcgag gaagaggcgc cttcgagcga ggactgcctg cgccacttcg atgcctgggg
      661 acgcccgcaa atccaaagct tcgatgtgca gttcgaccca ttccgcccgc accacgcgcg
      721 cttcaaagac aaggcgatga taagcctcgc cgtcgacggg aagctcagcc tcggcttttc
      781 tcggccgcag ttctcggcgg cttgggcggc ctattcgctg tatcccaagg cgtgcctgat
      841 ttcggcggtg gcggttccgt ggcggacgac tcccgagctg gctcagaaag tccgcgaccg
      901 cttcgtgaac gaaggcttca cgcagctcca gtcacttact gaagtgcggc cagttagaaa
      961 gtgagcgacg gggtgccggc tcgaaagtgc gcgggcgcgc cggtccggaa gtgagcgatg
     1021 ggcgctactt cgaaggtgag cgatgcgagg ggcgggctag ggggtgtccc aagttcctgc
     1081 ccgtcgcgag agccaagaaa cgcgagcgag aagcgcgccc gaggagtgag caccggagga
     1141 aggctgaagc cctttcgagg agccgagcga cgaggccgtg cttcgcagcc gcaggatctt
     1201 tggctcgcag cagggcgggg aacttgggac accccctaga acgcggccgc gatggcgctc
     1261 gcgacgtagc tgaccgcggc gaacaaccag acgctgctcc agacgtatcg gcgtgcctcg
     1321 aagccccagt acggagcgag cggaggcagg aaaaatgcgg ccaacgcgcg ccagcgaggc
     1381 ggttgggtgg cgagccccgc gacgagcacg acgtgcgcgg tgacgagcgc gcccaaggcg
     1441 gctgacagca cgagcatgcc gaagaggtcc acggcgcggg agcttacaat gacctgaaag
     1501 gtgcggggcg ccggcctttc ggctggaatc cgggcgaggg gtcgcgtggc tctcgggggt
     1561 tcgctacact cggcggaccg tgcgtttttc gtcgttgttg gtcgcactgt cactcggcgg
     1621 gttgctcttg ccgttaccgc tcgggctgcg gcgcgctcac gccgacgggc tgacgtcgcg
     1681 cgccgacatc atgccggtga aggacatcaa gcccggcatg aagggctacg ggctcaccgt
     1741 gttctccggc acgacgcccg agcgcttcga cgtcgaggtc atcgacgtgc tcacgaactt
     1801 ccgtccgcgt caggatctca tcctgatcaa gacgaagcat cctcggctcg aggtcgcgaa
     1861 ggtcgtcgcg gggatgagcg gcagcccgat ctacatcaac aacaagatga tcggcgcgta
     1921 cgcgtacggc tggacgttcg gcgccgaacc ggtcgccggc gtcacgccga ttcgcaatat
     1981 gctcgacgac ctggtgcgcc cgctgccacc ggacatctac ggctggaggc ttgcgacctt
     2041 gccgaagccg cgcgcgacga gctccggaaa gctcggtcac aacgaggcta cgccggacga
     2101 gcgactcgca ttcggggggt cgcgctttcg cggcccgagc tacgatttgc gagcgcatgc
     2161 ttccgagctc gcgagtcgtg gcgttcaggt gccggcggcg cagagcgccc tcgcgagcgt
     2221 ctccacgccg ctcttgctgt ccggcatgac cggcggcggc atgaacctcg cgcgtgattt
     2281 gctgcagccg ctcggcctcg agccgttgca ggcgggcgga gccggagctc ccgatccgaa
     2341 cgcgcccacc aagttcgtgg acggcggggc gatcggcgtg cagctcgtgc gcggcgacat
     2401 gagcgcgatg ggcctcggta cggtgacgcg cgtcgaaggc gaccgcttgg tggctttcgg
     2461 gcacccgatg atggagtcgg gcgtgaccgc gttacccacc gccattggca aagtgctgtg
     2521 gtttttggcg agcgatcaac gaagcttcaa gatcggcatg ccggtgcgcg agctcggtac
     2581 gctcgtcgac gaccggcagg cttcgatcgt cgtctcgcag tcgctgaaag cgccggtcat
     2641 cccgatgagc gtgaagatcc ggggagtgcc gggcctcgcg agctcgaatt ggaacttcga
     2701 ggtggcgcac gagcgcttct tgtcgccttc gttcgtgtcg gtggcttggg gcagcgcgct
     2761 gcagtccgtc gccgccgagc atcaagacgt gacgtggacc gcgaagagcg tcctcaagat
     2821 caaggactac ggcgagatca cgctcgacga ctacggcgtc gccatcggcg gaactcccga
     2881 caccggtgaa ctcagccgct cgaacttggt gcgcgccgtc ggcggcatca tgaacaaccc
     2941 gtggcagccg gtgatcatcg agtcgatcaa caccgagctc acactgacct tcgcccgcga
     3001 gatgttgcgg ctccgcggcg tcgacttggt ggacccggag gtcgatgcgg gtcaaccggt
     3061 gcgcctcaag ctcacgctcg cgccgtactc ggggcccgag atcacgcgcg tgctctctgt
     3121 ggacgtcccg aagacgctcg ccgggcaaaa cgtgaacatc gaggtcttgc cgggttacat
     3181 ggaggatcgc gaaggcgcgc cgccggacaa cttgtccgag ctcatcaaga acctcgaaaa
     3241 cccgacctac ccgccgcgct cggtggtcgt cgctttctcc gctggcaacg cggccgtgag
     3301 cttcaagggc cgcgtcgcga agaatctccc gccaggagct ctcgacgcgc ttcgtcccac
     3361 ggcgagctcg gtcggcccag atgcttttca aacagtggtt cgtaaggtgt ttcctctctc
     3421 cgagttcatg gtcggccgcg acaagctcag cgtcacggtg cggccggtgt tgcgctgaag
     3481 gagcgtgaag atgacttttc gacgcatttc acggttcttc tcggggctcg gggcggtgga
     3541 cgacagacgg ttcgcgatgc aggcaaagct ttccaactac gggctctttc gtggcattgg
     3601 cgcgctctcg gtggcgctcg gcgcgctcgt gtgcgcctcg cgcgctgacg ccgtcggcac
     3661 gcggcacttc ttgctcgaca agggcgccga cttcaaaggc ggcgacttga aaggcgtcgc
     3721 ggtcgactcg gcgggcggag tgcacgctgg cctcaccctc acggccgcac caatccaaaa
     3781 cgcggcgacg atttgggccg cgttgccgca gcgcgacggg tcggtgctgc tcggcaccgg
     3841 caacgaaggc aagctgatgc gcctcgctca gggagcgagc agcgtcgttg gtgagacgaa
     3901 gggcctcgcg gtgacgtcgc tggtcgaag
//
LOCUS       DASTKS010000373        12319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_952538, whole genome shotgun sequence.
ACCESSION   DASTKS010000373 DASTKS010000000
VERSION     DASTKS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952538"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFQ35_07695"
     CDS             complement(<1..336)
                     /locus_tag="VFQ35_07695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9930553.1"
                     /translation="MIFERGSVSKTITALAIMRLRDQVRLVLDAPAAAYLPQLSAVAA
                     PTTDSPPITVRHLLTMTSRVGYDDQWAASPTAKATRDSMRSSAAYLARSRAESYGLGW
                     VQHTTCLAEA"
     gene            542..1225
                     /locus_tag="VFQ35_07700"
     CDS             542..1225
                     /locus_tag="VFQ35_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930554.1"
                     /translation="MSHPIQPSLAIQNPAAYRARIKTFDHTELRRYLESLTREQLRNH
                     MRVMEPVKDRARGGFIGVYFRHGGGPWDPSIPFGVGDGFASSGKFPLTSRYAEYMSLK
                     RELAREVRVRLKFTIRFESEPFTLTRLENFMEVCQKFVASEVTLRRFVLAYFPESHER
                     GLTDPASVTLFAHLETEHGEDVHVELCTTAHPGYEERLVGWVRERASEFRVPFEQQGT
                     LRKRSNDTA"
     gene            complement(1461..2357)
                     /locus_tag="VFQ35_07705"
     CDS             complement(1461..2357)
                     /locus_tag="VFQ35_07705"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9930555.1"
                     /translation="MKTMALPPTLFVTGATGALGRLVIDALLETVPASSILAGVRDLD
                     KHEKAAAAFRARNIEARTADYTRPETLDRAFRDVDRLLLISSSENGKRKVQHRNVIEA
                     ARGAGVKLIAYTSILHADTTPLMLAEEHRDTEAALRASGVPFVLLRNGWYTEVYTWRL
                     PTALAHGVLAGAAGDGRISSAARADYARAAATVLTGDDHAGRIYELAGDASFTLAELA
                     AVVRAASGKPMVYRDMSTEAFRSAAIQAGVPEVVATIVSDTDAGVARGALFEDGGALR
                     RLIGRPTTPFQQTITDFVRAGS"
     gene            2438..2875
                     /locus_tag="VFQ35_07710"
     CDS             2438..2875
                     /locus_tag="VFQ35_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9930556.1"
                     /translation="MGQGNIEVTSSEPERRKSWAVDPQCPVRDVLDRIGDKWSTLVLV
                     TLADGPRRFSEVRRAISDISKRMLTQTFRDLERDGLIARTVFPTKPPSVEYRLTPLGE
                     TILEPLRALADWANRNHEAIKNARLAFDANVVSEPINPPAKGC"
     gene            complement(2960..4669)
                     /locus_tag="VFQ35_07715"
     CDS             complement(2960..4669)
                     /locus_tag="VFQ35_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930557.1"
                     /translation="MLRAALTGGTLGAVVSLAAAGAAWALYVGPPRLFTLLACGAGAL
                     VGAAWGVRQRWSHVNVALFLDARLGESELVTSALDRANDADPVANALRERAARSLHAF
                     SAQRLDLSPFSRWHALMLPALGLAAWVSVLPARVAVAASPPGAKLLQRKNVPGLERIE
                     ALSNAPSLSDSDAERLKRLAEEARRLRSELGKGLSQREAQARIAALRESIGKERERFG
                     DRTERPGLEAAIGALEAETTTKNLAKALGDGDLVTFDEEMQRIANLAESESRKAAERA
                     LEEAARVAREKAAKKLSDYLDRKRKDFREREANARTLREFAKELESKLSPEARSDLKQ
                     FEQSGDPEAARRLAEALGKALKGLSEEERQKLADALKNKMNEHGEDVSEMSPEEMRRL
                     VDSLKSAEGQKKLEDALRDLAKKGADSERDRALDDAERGGAEAERSLGGNPTPMPMPG
                     SGDSSANGGPKGREGTGKDDARSKGPGQNGDGPGGPAKHDGTTDKLDSNELRARANAR
                     WLPGAPLAARSLGRAPGRAGETANQVGVGNLSSRASSEVGAVERGDIPEEYREQVGRY
                     FEP"
     gene            4925..5827
                     /gene="panB"
                     /locus_tag="VFQ35_07720"
     CDS             4925..5827
                     /gene="panB"
                     /locus_tag="VFQ35_07720"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9930558.1"
                     /translation="MSKVTVPEIRARKQRGQKLAVLTAYDATIARLLDRGGADVLLVG
                     DSLGMVVQGRENTLPVTIEEICYHGRAVARGTRRAHIVGDMPFMSFQLSAQHALESAG
                     RMMKDGAFEAVKLEGGERYVEHVRAIVGAGIPVMAHLGLLPQSVHTMGGFRVQGRDDA
                     SAERLIRDARLLEEAGAYAVVLEGIPAELARRVTEAVSIPTIGIGAGPYCDGQVLVCY
                     DFLGMYSEVRPKFVKRFAELGDAVVEATQRYVAEVQSSAFPGAEHSFGGATPKPRPPQ
                     EDTGARPVVDGGPTTYGPAADEPS"
     gene            5824..6705
                     /gene="panC"
                     /locus_tag="VFQ35_07725"
     CDS             5824..6705
                     /gene="panC"
                     /locus_tag="VFQ35_07725"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9930559.1"
                     /translation="MKPTVYETASEFRAAMDGLRGAGARIGLVPTMGALHSGHLALAE
                     FARGRGATPALTIFVNPTQFGPNEDFARYPRTLERDLGLCAERGVSHVFVPSVSEMYP
                     EGERTRVRVDKLTDVLCGPRRPNHFEGVATVVAKLFNAAGPCLAVFGRKDYQQLQVIR
                     RMARDLLFPVEVVGHPIVREADGLALSSRNAYLDAPARALAPRIYQELSRAARAFASG
                     ERRAAELRVPVERALSSAGFRVDYVELVTAESLEAIADDARVPERALLAVAAFLGATR
                     LIDNFVLGEDEVFGAGS"
     gene            6702..7355
                     /gene="tmk"
                     /locus_tag="VFQ35_07730"
     CDS             6702..7355
                     /gene="tmk"
                     /locus_tag="VFQ35_07730"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9930560.1"
                     /translation="MNTSGRFIVLEGVDGCGSTTQTRRLVEALRNRGHDARATCEPSD
                     GPIGVLIRKALEKRLTDGEGAPRRFDWATLALLFAADRVDHVANGIDPALQASAVVVS
                     DRYTLSSVVYQSVTAPEGTPDAVRWISEINRAARSPDVTIVLGVSAQVAAERRRKRGG
                     PEELFDAMSLQERLAQAYAKAEQLAPDQRIVHVSGEGSAEEVTERLLEVVRAFLPNP"
     gene            7426..7926
                     /locus_tag="VFQ35_07735"
     CDS             7426..7926
                     /locus_tag="VFQ35_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930561.1"
                     /translation="MRTLFIALVALVGCSSTPVLEGPAPAMAPPPPAVPPPSLVAAPR
                     GKLFRDEVASTVDRGFGMFLQKLKVEANVRDGKFAGWIVRALYPRDFWDGVDLQTGDV
                     ITQVNDKPIERDIQAFEVFQSLKTAPRLRVRYVRDGETRTLDYEIIERGASAPVASAS
                     TQPAAN"
     gene            complement(7908..9044)
                     /locus_tag="VFQ35_07740"
     CDS             complement(7908..9044)
                     /locus_tag="VFQ35_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930562.1"
                     /translation="MSAPHRVYFVPGMFGFGHLAGYDYFNHVRRGLEQRYGAAGIPVV
                     FEDVPAPPTSSLRYRSRVLATTVARSVSRDDGPIHFVGHSTGGLDVRLVLSPSANLGL
                     PPELLGFRSRVASAVTMNTPHYGTPLASYFATVSGTRLLYALSLLTVVSLSLGEPSLN
                     IFSRLLSGVGSIDNLFGGDLKLVSRITDSILRFVDKDGRQEIHEFLSQVRVDQGGVIQ
                     IMPEAMDLFNAATENQLAVRYACVASAAPAPSLLRAARRLRSPYAALTGAIYTTLYQF
                     TSQRPKVYPYAQPTPREASLMRAGIPQDVDESSNDGIVPTLSMLWGELLWAGEADHLD
                     VLGHFHDEITPSVHTDWVTSGARFTRQRFAALLDAIAAFQLAAG"
     gene            complement(9069..9950)
                     /locus_tag="VFQ35_07745"
     CDS             complement(9069..9950)
                     /locus_tag="VFQ35_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930563.1"
                     /translation="MGATVLRAALATIGMAVSACSASTSSYTSDAERGSTHALITVER
                     RDTLSSAAVSDARAFATFVRTPPEVDIATVTRLAGLDLTLPEPGDCALGGTHKEVASL
                     SSPLRRVELMAAGDVALETPASRVELAPRAFPAVTDLFAGVVYTTRDRAALLPTGEVY
                     AVTTAGSSSLAPLSVSTEAPQGLSSLTLSGQPLSSTSILPASGAELGWRAGSARDLVY
                     VALSTSDGTPYAVCAFRDDAGRGIIPASAVPASSSVSLAVHRLRSVTLPASTGGIDAG
                     ELRFDFEQSTSVSVQGR"
     gene            10386..10937
                     /locus_tag="VFQ35_07750"
     CDS             10386..10937
                     /locus_tag="VFQ35_07750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9930564.1"
                     /translation="MIIPGSKLPEFSTQACVSIEKGKEFKKITSKDFPGKWLVLYSYP
                     KDFTFVCPTEIVDFDKKVKAFADRDAVVLGGSTDNEHSHLAWRLQHADLKGLNHALLF
                     YSPALAQQLGILHPEAGVALRATIIVDPEGVVRFATANDLSVGRNVEEILRVLDALQT
                     DELCPCNWKKGEETLTQQMKKTG"
     gene            complement(10989..11294)
                     /locus_tag="VFQ35_07755"
     CDS             complement(10989..11294)
                     /locus_tag="VFQ35_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HET9930565.1"
                     /translation="MATRITDDCINCGACEPECPNSAISQGDDIYVIDPELCTECVGF
                     YDHEACQAVCPVECCLPDPNHVEAEDTLVKRALELHPDDSELSARVASGAFPSRFRK"
     gene            11348..11962
                     /locus_tag="VFQ35_07760"
     CDS             11348..11962
                     /locus_tag="VFQ35_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930566.1"
                     /translation="MVRAPLTFSIALVAFGAVACGHNIGDDCKTSVDCSQAGERLCDI
                     TQPGGYCTVFNCEPNTCPSEAACVVFDPQLSAAAECKATNGLSRFARSFCLFRCDSNG
                     DCRDGYVCQDFSREEPNDWNGLAVDTDRSGKVCVARRDVQSVHVDSLPPTNVCDGPPP
                     SGGATASGGSPSASGGSSGAGGSSGLGGAAGAGGAEAGAGGAGG"
     gene            11962..>12319
                     /locus_tag="VFQ35_07765"
     CDS             11962..>12319
                     /locus_tag="VFQ35_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9930567.1"
                     /translation="MFELGLLPRSLSAALRDVGNPRTEVRLSALRDLARYARQGEREA
                     GSALLDALKDPDEAVRAAAALGLADANLSFAAARLVELAENDASSRVRQMALLGLGEL
                     ASSDHGAVLDALWKALS"
BASE COUNT         2018 a         4056 c         4081 g         2164 t
ORIGIN      
        1 cgcctccgcc aggcacgtgg tgtgctgaac ccaaccgagt ccatagctct ctgctcgcga
       61 tctcgcgagg tacgccgccg acgaacgcat cgagtcccgc gtcgcttttg ccgtaggtga
      121 cgcagcccat tggtcgtcgt agccgactcg actcgtcatc gtgaggaggt ggcgcaccgt
      181 gatgggcggc gaatcagtcg tcggcgcggc gaccgcactc agctgcggca ggtatgcagc
      241 cgccggagca tcaaggacaa gcctcacctg gtcgcgaagc cgcatgatgg caagggcggt
      301 gatggttttg gaaacggagc cgcgctcgaa aatcacgggg gagttcaggg gcaataggag
      361 acagctctca gcatcgaaag cgacgggcgg cgcacccagt tgtcatatcg cgcggcaaaa
      421 gcgtcgcttg tacggcctaa gcacagaacg gagcgcccaa caaagcccca tgactcacgc
      481 tctgacacgc tgcatgctgg agagctgccc tcgcgtcacg tatgcctgga gttctgaggc
      541 catgtcacat cccatccaac catcgctcgc gattcagaat ccggctgctt accgggcccg
      601 catcaaaact ttcgaccaca cggagctccg gcgctatctc gagagcctca cccgagagca
      661 gctacgcaac cacatgcgtg tgatggagcc cgtgaaggat cgcgcgcgcg gcgggttcat
      721 cggggtgtat ttcaggcacg ggggcgggcc gtgggacccg tcgatcccgt tcggtgtcgg
      781 cgatggcttc gctagctcag gcaaattccc actcacgtcg aggtacgctg aatacatgtc
      841 gttgaagcgt gagctcgcgc gtgaagtgcg cgtgcggctc aagttcacga ttcgattcga
      901 atccgaacca ttcaccttga cccgcctcga aaacttcatg gaggtctgcc agaagttcgt
      961 cgcaagtgag gtcacgctgc gccgcttcgt cctcgcgtat tttcccgagt cccacgagcg
     1021 cggtctcacg gacccggcat ccgtcactct cttcgcccat ctcgagacgg aacacggcga
     1081 agacgtccac gttgaactct gcacgactgc ccaccccggg tacgaagaac ggctcgtcgg
     1141 ctgggtccgt gagcgtgcct cggagttccg cgtccccttc gaacaacaag gaacattacg
     1201 aaagcgatcg aacgacacgg cttgagttgt cactcctcgc gctcttgatc tggtcttcga
     1261 tttcgacgag cgtcgtagta ctcgcgagac cgtgggaagc cggcgtctcg ggttgtcacg
     1321 agacgcacgc gcggaaaagc cgaggtgcgc atagtgcgag cgcgagacgc acgcatgacg
     1381 acagcgcccg catttccctt cacgccctac gaatcgagcc ctccccccta cccccgtcgg
     1441 cacgaacgct cgggttggcg tcacgagcct gcacgaacga aatcggtgat cgtttgctgg
     1501 aatggggtcg tggggcgacc gatgagccgc cgaagtgcgc cgccatcttc gaacagcgcg
     1561 ccgcgtgcga ctccggcatc cgtgtcggac actatcgtcg cgaccacctc cgggacgccc
     1621 gcctgtatcg ccgcggaccg aaatgcttcc gtggacatgt cccgatagac catcggcttt
     1681 cccgacgctg cgcggacgac cgcggcgagc tccgctagcg tgaacgaagc gtcgcccgcg
     1741 agttcgtaga tgcgccccgc gtgatcgtct cccgtcaaca cggtcgctgc cgcgcgcgcg
     1801 tagtcggcac gcgccgcgga agagatccgc ccatcgcccg ccgcaccagc gagcacgcca
     1861 tgggcgagcg cggtggggag ccgccacgtg tacacctcgg tgtaccagcc gtttcgcaac
     1921 aggacgaacg gcacgccaga cgcccggagc gccgcctccg tgtcgcgatg ctcttccgcg
     1981 agcatcagcg gcgtcgtgtc cgcatggaga atgctggtgt aggcgatgag cttcacccct
     2041 gctcctcgcg cggcttcgat cacgtttcga tgctgcacct tccgcttgcc attctcgctg
     2101 ctcgaaatga gaagcagccg gtcgacgtcg cggaacgcgc ggtccagcgt ctccggtcgc
     2161 gtgtaatcgg cggtgcgagc ctcgatattc ctcgcccgaa atgcggccgc cgctttttcg
     2221 tgcttatcga ggtcgcgtac gcccgcgagg atcgagctcg ccgggaccgt ctcgaggagc
     2281 gcgtcgatga cgagccggcc gagtgcaccg gttgcaccgg tgacgaacag ggttggcggg
     2341 agggccatgg tcttcattct cctgcgttcc gggggtaagg tttcggaacg cacggcaaac
     2401 gtaagcaaga cccgcgcggc gtgaaggagg caggattttg ggacagggta acatcgaagt
     2461 aacctcctcc gaacccgaac gccgcaagag ctgggcggtc gacccgcagt gcccagtgcg
     2521 agacgtgctc gaccgcatcg gcgacaagtg gtcgacgctc gttctcgtca cgctcgcgga
     2581 cggaccgcgc cgcttcagcg aggttcggcg tgcgatctcc gacatctcga agcgcatgct
     2641 gacgcagacg tttcgggatc tcgagcgcga cggcctcatc gcacgcaccg tgttcccgac
     2701 aaagccgccg agcgtcgagt accggctgac gccgctcggc gagacgattc tcgagcctct
     2761 acgggccctc gccgactggg ccaatcgtaa tcacgaagcg atcaagaacg cccgtctcgc
     2821 gttcgacgcg aacgtggtga gcgagcctat caatccacct gccaaaggtt gctaaggggc
     2881 ccgcctcgat gcttgcttct gcgcgtcgtg tgtgcctggc attggtctgt cgtgaagcag
     2941 ggcgtttttc aacgccctgc tagggctcga aatagcgacc cacctgctcg cggtactctt
     3001 cgggaatgtc cccgcgctcc actgcgccca cttcggagct ggcgcggctg gacagattgc
     3061 cgactcccac ttggttcgca gtttctcccg cgcgacccgg cgcgcggccg agcgaacgag
     3121 cggccaatgg cgctccgggc aaccagcgcg cgttggctcg agcgcgcagc tcgttcgaat
     3181 cgagcttgtc ggtcgtcccg tcgtgcttgg caggcccgcc ggggccgtcg ccattttggc
     3241 caggtccctt cgagcgagcg tcgtctttgc cggtgccctc gcgtcccttg ggtccgccgt
     3301 tcgcgctgct gtcgccgctg cccggcatcg gcatgggcgt cgggttgcct ccgaggctcc
     3361 gctccgcttc cgcaccgcca cgctcggcgt cgtcgagcgc gcgatctcgc tccgaatcag
     3421 cgcctttctt ggcgaggtcg cgcagcgcat cctcgagctt cttctggcct tcggcgctct
     3481 tcagtgagtc gacgagccgg cgcatctcct cggggctcat ttcggagacg tcttcgccgt
     3541 gctcgttcat cttgttcttc agcgcgtcgg cgagtttttg ccgctcttcc tcgctgagtc
     3601 ccttcagcgc tttgccgagc gcctcggcta gccgccgcgc cgcttcgggg tcgccgctct
     3661 gctcgaactg cttcagatcc gagcgcgctt cgggcgacag cttgctctcg agctccttcg
     3721 cgaactctcg caacgtgcgc gcgttcgcct cccgctcgcg aaagtctttg cgttttcgat
     3781 cgaggtagtc ggagagcttc ttcgccgctt tttcgcgcgc aacgcgagcg gcctcttcga
     3841 gcgcgcgctc ggccgctttg cgcgattcgc tctcggccag gttcgcgatg cgctgcatct
     3901 cttcgtcgaa ggtcaccaag tcgccgtcgc cgagcgcttt cgccaaattc ttcgtcgtgg
     3961 tttcggcttc gagggcgccg atggctgcct ccaagccggg gcgctcggtt cggtcgccga
     4021 agcgctctcg ctccttgccg atgctctcgc gaagcgctgc gattcgtgct tgcgcttcac
     4081 gctggctgag ccctttgccg agctccgaac gcagccggcg cgcctcttcc gcgagccgct
     4141 tgaggcgctc ggcgtcgcta tcggacaggc tcggcgcgtt ggagagcgct tcgatgcgct
     4201 cgaggccagg gacgttcttg cgctgcaaga gcttggcgcc gggggggctt gccgcgacgg
     4261 ccacgcgcgc cggcagcacc gagacccacg ccgcgagacc cagcgcaggc agcatcagcg
     4321 cgtgccaacg cgagaacggc gacaggtcga gccgctgcgc cgaaaaggca tgcagcgagc
     4381 gcgccgcgcg ttcgcggaga gcgttcgcga cgggatccgc gtcgttcgcg cggtcgaggg
     4441 cgctcgtcac gagctccgac tcgccgaggc gcgcatccaa gaatagagcc acattcacgt
     4501 ggctccagcg ctggcgaacg ccccaagcag ccccgaccaa ggctcccgcg ccgcacgcga
     4561 ggagcgtgaa aaggcgaggg gggcccacgt agagcgccca ggccgctccc gccgccgcga
     4621 gcgagaccac cgcgcccaaa gttccgcccg tgagagctgc ccgaagcaat cgacgcgttt
     4681 cgatgactcg cccccaacgg cgaatctcgc gggcaaatcc ggagtcagcg tgcagcggtg
     4741 ccagaatctt cagggtaact cccgccccag gccaggtcca gcgcggcact gtttttgggg
     4801 gccgcggtgt cgccatccac gctgatccgc gaacctgctc atccgagaat ggcgacgatg
     4861 aggctcgagg caggcttccg tttgtcgccg attgatgcaa gctctccctg agagaggtgc
     4921 gttcatgagc aaagtcaccg tgccggagat tcgcgcgcgt aagcaacgcg gccagaagct
     4981 tgccgtgctc accgcctacg acgcgaccat tgcgcgcttg ctcgatcggg ggggcgcgga
     5041 cgtgctgctc gtgggcgact cgctcgggat ggtcgtccag ggacgcgaga acacgctgcc
     5101 cgtgacgatc gaggagattt gctaccacgg tcgcgcggtg gcgcgaggta ctcgacgcgc
     5161 ccacatcgtc ggtgacatgc cgttcatgag cttccaactg tcggcgcagc acgcgctcga
     5221 gtcggctggg cgcatgatga aggacggcgc cttcgaggcg gtgaagctcg agggcggcga
     5281 acgctatgtc gagcacgtgc gcgccatcgt cggcgcgggg atcccggtca tggctcacct
     5341 cgggcttttg ccccaatccg ttcatacgat gggtggcttt cgggtgcagg ggcgcgatga
     5401 tgcgtcggcc gagcgcttga tccgcgacgc gcggctgctc gaagaggctg gcgcgtacgc
     5461 ggtcgtgctc gagggcatcc ccgccgagct cgctcgtcga gtcaccgaag cggtgagtat
     5521 ccccaccatc ggcatcggtg cggggccgta ctgcgacggt caggtgctcg tttgctacga
     5581 cttcctcggg atgtactcgg aggtgcggcc caagttcgtg aagcgcttcg cggagctcgg
     5641 tgacgcggtg gtcgaagcga ctcaacgcta cgtcgccgaa gtgcaaagca gcgcttttcc
     5701 cggcgccgaa cactcctttg gcggagccac accgaagccg cgccctccgc aggaagacac
     5761 cggcgcgcgc cccgttgtcg acggcggccc gacgacgtac ggtccggccg cggacgaacc
     5821 ttcatgaagc ctacggttta cgagactgcg agcgagttcc gcgcggcgat ggacgggctg
     5881 cgcggcgcgg gagcccgaat cggcctcgtc ccgaccatgg gcgccctcca ttccggccac
     5941 ctcgcgctcg ccgaattcgc gcgtggccgc ggggccactc cggcgctcac catcttcgtg
     6001 aacccgaccc aattcggacc gaacgaagac ttcgcgcgct atcctcgcac gctcgagcgc
     6061 gacctcgggc tctgcgccga gcgcggcgtt tcgcacgtgt tcgtgccgag cgtgtcggag
     6121 atgtaccccg aaggcgagcg gactcgggtg cgggtcgaca agctcaccga cgtgctgtgc
     6181 gggccgaggc gcccaaacca tttcgagggt gtcgccaccg tcgtggcgaa gctgttcaac
     6241 gccgccggtc cttgcctcgc ggtgttcggg cgcaaggact accagcagct tcaagtgatc
     6301 cggcgcatgg cgcgggatct gctatttccc gtcgaagtcg tggggcaccc catcgttcgc
     6361 gaagccgatg ggctcgcgct gagttcccga aacgcctacc tcgacgcgcc ggcgcgcgcg
     6421 ctcgctcccc gaatctacca ggagctctcc cgcgccgcgc gagccttcgc ctcgggtgaa
     6481 cggcgtgccg cggagctcag agttccagtc gaacgagccc tttcatccgc aggctttcgc
     6541 gtcgactacg tcgagctcgt cacggccgag tcgctcgagg ccatcgccga cgacgcgcgg
     6601 gtgcccgagc gggcgctgct cgcggttgcc gcctttctcg gggcgacgcg cctgatcgac
     6661 aacttcgtgc tcggcgagga cgaggtcttc ggagccggtt cgtgaacacc tcggggcgct
     6721 tcatcgtgct cgaaggggtg gacggctgcg gcagcacgac tcaaacccga cggctcgtcg
     6781 aggcgttgcg caatcgtggt cacgatgcgc gcgccacttg cgaaccgagc gacggcccca
     6841 tcggagtact catccgaaag gcgctcgaaa agcggctcac cgatggagag ggcgcgccgc
     6901 ggcggttcga ttgggcgacg ctcgcgttgc tcttcgccgc ggaccgcgtg gatcacgtcg
     6961 cgaacggcat cgaccccgcg ttgcaagctt ccgctgtcgt cgtgagcgat cgctacactc
     7021 tgtcgagcgt cgtttatcag tcggtgaccg cccccgaagg tacaccggac gcggtgcgct
     7081 ggatctccga gatcaatcgt gcggcgcgtt cacccgacgt gacgatcgtg ctcggtgtga
     7141 gcgcgcaggt ggcggctgag cggcgccgga agcgcggcgg ccccgaggag ttgttcgacg
     7201 ccatgtcgct gcaagagcgt ctcgcgcagg cttacgcaaa ggccgagcag ctggcgcccg
     7261 accagcgcat cgtgcacgtc tcgggggagg ggagcgccga agaagtcacc gagcgcttgc
     7321 tcgaggtggt tcgggcgttc ttgccaaatc catgatcttc tgtgcgttta gggcgcgccg
     7381 ctcgggattt ggccgcgaac tttcgcctct gccatagtga ggcgcgtgag aacgctgttc
     7441 atcgcgctcg tggcgttggt tggttgtagt tcgaccccgg tgctcgaagg gccggctccc
     7501 gccatggctc ctccgccccc ggcggtcccg ccgccttcgc tcgtcgccgc tccgcgcggc
     7561 aagctattcc gcgacgaagt ggcgagcacg gtcgaccgag ggttcggcat gttcctccaa
     7621 aagttgaagg tcgaagccaa cgtgcgcgac ggcaaattcg ccggttggat cgtgcgggcg
     7681 ctgtatccgc gcgacttctg ggacggcgtc gacctgcaaa cgggggacgt catcacgcaa
     7741 gtgaatgaca agccaatcga gcgggacatc caagcgttcg aggtcttcca gtcgttgaag
     7801 accgcgccgc ggctccgagt gcgctacgtg cgagacggcg agactcgcac cctcgattac
     7861 gagatcatcg agcggggcgc gtcggcgccg gtcgcgagcg cgtcgactca gcccgccgcc
     7921 aactgaaacg ccgcgatggc gtcgagtagg gccgcgaagc gctgccgcgt gaagcgagcg
     7981 ccgctcgtga cccaatcggt gtgaacgctg ggcgtgattt cgtcgtggaa gtgcccgagc
     8041 acgtcgaggt ggtcggcttc gcccgcccac aagagctctc cccaaagcat gctcagcgtc
     8101 gggacgattc cgtcgttcga gctctcgtcg acgtcttgcg ggatgccggc tcgcatcagc
     8161 gaggcctcgc gcggcgtcgg ctgcgcgtaa ggatagacct tcgggcgttg gctcgtgaat
     8221 tggtagagcg tcgtgtagat ggctccggtc agcgccgcgt acggcgagcg tagccggcgc
     8281 gcggcacgca gcaggctggg cgccggcgcg gcggacgcga cgcaggcgta gcgcaccgcg
     8341 agctggttct cggtcgcggc gttgaagagg tccatcgcct ccggcatgat ctggattacg
     8401 ccgccctgat cgacgcgcac ctggctgaga aattcgtgga tttcctggcg gccatccttg
     8461 tcgacgaagc gcaggatcga atcggtgatg cggctgacga gcttcaagtc gccgccgaac
     8521 aggttgtcga tgctcccgac gccggagagc aggcgcgaga agatgttgag cgacggttcg
     8581 ccgagcgaga gcgaaacgac ggtgagcagg ctgagcgcat agagcaggcg cgtccccgat
     8641 acggtcgcga agtagctcgc gagcggcgtt ccgtagtggg gggtgttcat cgtcaccgcg
     8701 ctcgcgacgc gcgaccgaaa gccgagcagc tccgggggca gccccaaatt tgcgctcggt
     8761 gagagcacga gccgcacgtc gaggcctccc gtcgaatgtc caacgaagtg aatcggtcca
     8821 tcgtcgcggc tcacgctgcg cgcgacggtg gtcgccaaca cacgcgagcg atagcgaagt
     8881 gacgaggtgg gcggcgccgg cacgtcttcg aagacgactg ggatccccgc ggcgccatag
     8941 cgctgctcga gcccgcggcg aacgtggttg aagtagtcgt agcccgcgag gtgcccgaaa
     9001 ccgaacatac ctgggacgaa gtagacgcga tgcggagcgc tcatcggcgg cgcggctcga
     9061 atcccaactc agcgaccctg gacactcacg ctggtcgact gctcgaagtc gaaacgcagc
     9121 tcaccagcgt cgatccctcc ggtgctcgcg ggtagggtca cgctccgcag cctgtgaacg
     9181 gctaggctca cggaactgct tgcgggtacg gcgctcgcgg ggatgatgcc gcgcccagcg
     9241 tcgtcacgga atgcgcacac cgcgtacggc gttccatcgc tcgtgctcag ggcaacgtac
     9301 accaaatcgc gcgcgctccc cgcccgccac ccgagctctg cgccgctcgc cggcaagatg
     9361 ctggtcgagc tcaacggctg accagaaagg gtcaggctgc tgagcccttg cggtgcttcg
     9421 gtgctcaccg aaagcggtgc gagagaagag ctccccgcgg tcgttaccgc gtagacctct
     9481 cccgtgggaa gcaatgccgc gcggtcacgc gtcgtgtaga cgacgcccgc gaacaagtca
     9541 gtcactgccg gaaaagcgcg cggtgcgagc tcgacgcgac tcgccggagt ctcgagcgcg
     9601 acgtcaccag cagccatgag ctcgacgcgt cgcagcgggc tcgataggct ggcgacctct
     9661 ttgtgggtgc cgccgagcgc gcagtctccc ggctcgggca gcgtcaaatc gagcccggcc
     9721 aagcgagtca cggtcgcgat gtcgacctcg ggtggagtgc gcacgaaggt cgcgaacgcg
     9781 cgcgcatcgg acacggccgc ggaactcaag gtgtcacgtc gctcgaccgt gatgagggcg
     9841 tgcgtactgc ctcgctctgc gtccgaggtg taagagctcg tggacgcgga gcacgccgac
     9901 acggccatcc cgatggtggc taaggctgct cgcagaacgg ttgctcccac ccgctaacca
     9961 tattcaggcc gctgctttcc ggcaagcgcg caaagaactc ttggtggggg tttcacgcag
    10021 agctggccca ctctcgagcg acggccgccg acgctccgtg cgttccacca tccccgcatt
    10081 tttggtgttc actaggtcgt tacgtgagtg gcgggagttc ttcgtgcgct cgccacgccc
    10141 cgatgaaacc gccgcagggc aggcgagccc atgttgggca caaagcgtcg gtgtcggggt
    10201 ggtcccgctc gcccgccagt accgaagctg actttttggt gatgcccccg aataagaacg
    10261 atttgggggt ttgacgcaca ctaggcttta gactaagtct acatccagat agtttccatg
    10321 gcgggattca cctacctgaa gccccgtcag cccacccaac tgaagatcca attaggagag
    10381 tttccatgat catccccggt tcgaagcttc ccgagttcag cacccaggcc tgcgtcagca
    10441 tcgaaaaagg caaagaattc aagaagatca cgtcgaagga cttcccgggt aagtggctgg
    10501 tcctctacag ctacccgaag gacttcacct tcgtttgccc gaccgagatc gtcgacttcg
    10561 acaagaaggt caaggcgttc gcggaccgtg acgcggtcgt cctcggcggg tcgaccgaca
    10621 acgagcactc gcaccttgcc tggcgtttgc agcacgccga tctcaagggc ttgaaccacg
    10681 cgctcctctt ctactcgccc gcgcttgccc agcagctcgg catcctccac cccgaggcag
    10741 gcgtcgccct ccgcgcgacg atcatcgtag accccgaagg cgtggtgcgc ttcgcgacgg
    10801 cgaacgacct ctccgtcggt cgcaacgtcg aggagatcct gcgcgttctc gatgctttgc
    10861 agaccgacga gctctgcccc tgcaactgga agaagggtga agagactctc acccagcaga
    10921 tgaagaagac cggctgagcg agccgcgcgg tgtccggagt tcgggccgct ctggcggctc
    10981 gacccggact acttgcgaaa acgcgatgga aaggcgccgg aggcgacacg ggcggagagc
    11041 tcgctgtcgt ccgggtgcag ctcgagcgcc cgcttgacca gcgtatcctc cgcctccacg
    11101 tggttcggat cgggcaggca gcactcgact gggcagactg cctggcaggc ctcgtgatcg
    11161 tagaagccga cgcactcggt gcagagctca gggtcgatga cgtaaatgtc gtcgccctgc
    11221 gaaatcgcgc tgttggggca ctcgggctcg caggcgccgc agttgatgca atcgtcggta
    11281 atgcgcgtgg ccattaggtt ggccaatgta gcgcaggctg ccaacggcgt gtagagtgcc
    11341 gcgccccatg gttcgcgctc ctctgacttt ttcaatcgct ttggttgcgt tcggcgcggt
    11401 ggcgtgcgga cacaacatcg gcgacgactg caagacctcg gtcgactgct cccaagctgg
    11461 cgagcgactg tgtgacatta cgcagcctgg cggctactgc acggtgttca actgtgagcc
    11521 gaacacgtgc ccatcggagg cggcctgcgt cgtgttcgac ccgcagcttt cggcggcagc
    11581 ggagtgcaaa gcgacgaacg gcctgtctcg cttcgcgagg agcttctgcc ttttcaggtg
    11641 cgactccaat ggcgactgtc gcgacggcta cgtgtgtcag gatttttcgc gcgaagagcc
    11701 gaacgattgg aacggcttgg cggtcgacac ggatcgtagc ggcaaggtgt gcgtcgcgcg
    11761 ccgtgacgtg cagagcgtgc acgtcgactc gctacccccc accaacgtct gcgacggtcc
    11821 gcctccgagc ggcggcgcca ccgcgagcgg cggttcgccg agcgcgtccg gtggttcgtc
    11881 cggcgccggt ggctcgtccg gtctcggtgg cgcggcgggt gctggcggag ccgaagcggg
    11941 cgcaggtggc gcgggcggct gatgttcgaa ctcggattgc ttccgcgctc gctgtctgca
    12001 gcgttgcgtg acgtcggcaa tccgcgtacc gaggttcgcc tttcggcgct gcgcgatctg
    12061 gcgcgatacg ctcggcaagg tgagcgcgaa gcgggctcgg ccctgctcga tgccttgaag
    12121 gatcccgacg aggcggtgcg cgcggcggcc gcgctcggtt tggctgacgc caatctgagc
    12181 ttcgccgcgg cgcggctcgt ggagctcgcc gagaacgacg cgtcgtcgcg cgtgcgccag
    12241 atggcgctgc tcgggttggg agagctcgcg agcagcgatc acggcgcggt cctcgatgcg
    12301 ctctggaaag cgctttctg
//
LOCUS       DASTKS010000374         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1399961, whole genome shotgun sequence.
ACCESSION   DASTKS010000374 DASTKS010000000
VERSION     DASTKS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399961"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFQ35_07770"
     CDS             complement(<1..521)
                     /locus_tag="VFQ35_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9930568.1"
                     /translation="MSRQTKAVLVAGAGPTGLMLANCLAVYGVPFDIVDRKAEPSCDS
                     KALAINILSQYALALIGKGDALGEQGRRVRRANVFWNGTRLNAFDFRWLDFELKSFVT
                     QPQAATERELAAALPRDVEIAWRTELVEIAERGDHVHATFRDAAGRTFERAYDYVVGC
                     EGKNSLVRESIGA"
     gene            667..1020
                     /locus_tag="VFQ35_07775"
     CDS             667..1020
                     /locus_tag="VFQ35_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9930569.1"
                     /translation="MPKRQRYLFVCSNRRQDGAPKGSCAARGSLELHAKLKALLQERG
                     LAEFAVRACTTSCLDACWTGPTIAVEPDHYFYGRVRDSDLGDIVDALATGTRVERLVL
                     KPEDYLEPRERKKLS"
     gene            1051..>2146
                     /locus_tag="VFQ35_07780"
     CDS             1051..>2146
                     /locus_tag="VFQ35_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9930570.1"
                     /translation="MQKVHEAAVDAKIAVATMSLLEPPPTVPLDSILPAEPLLLMGAG
                     PVPIPHAVARANGVVINHLGETMDRVIRSLKVMARYAFQTRSERILGVAGPASAAMEM
                     AVSNLLWPGRRALCLKTGTFSGRLGEMATGVGADVRTITTDTAKPILAERVARALAEE
                     HFDVVMLAQGETSCGVLNAELPAIAKLCRDHGALLIVDAVVTLSTMPVEMDGWGLDAV
                     VTGGQKGLSSIPGVSLLAFSERAWEAIERRTAPMPHWCFDARRAERFWAHQQYHYTAP
                     VPGVLALYEALRLVCEEGLEARFERHRTSSISLQAGLSAMGLELFVPAEHRLNSVVAI
                     GVPRGVDAPQVLSFMSRKFRVEISGAFGLEI"
BASE COUNT          367 a          728 c          682 g          369 t
ORIGIN      
        1 ttggccccga tgctctcgcg caccaggctg ttcttacctt cgcagcccac cacgtaatcg
       61 taagctcgct cgaaggtgcg ccccgccgcg tcgcggaacg tcgcgtgcac gtgatcgccg
      121 cgctcggcga tctccacgag ctcggttcgc cacgcgattt cgacatccct tggaagcgcg
      181 gccgcgagct cgcgctcggt ggcggcctgt ggctgcgtca cgaacgactt cagctcgaaa
      241 tcgagccacc gaaagtcgaa tgcattgagc cgtgtgccgt tccagaacac gttcgcgcgc
      301 cgcactcggc ggccctgctc gcctagggcg tcacccttgc cgatcaacgc gagggcgtac
      361 tgagagagga tgttgattgc gagcgcctta ctgtcgcagc tgggctcggc cttgcgatcg
      421 acgatgtcga acggcacgcc gtagaccgcg aggcaattcg cgagcatgag cccggtcggg
      481 ccggctcccg ccaccaatac cgctttcgtc tgacgcgaca cagtcatgcg aatagtcccc
      541 atcgcaagtt aacccccgcc gcacaccttg gtaggaggca cgatgtcccg tgcccgggct
      601 ctcgtcgagg gggacaacat gccctctccc acaccagcgt tcgcagagag aacgtctcct
      661 tcctagatgc cgaagcgcca acgctacttg ttcgtctgca gcaaccgccg acaggacgga
      721 gcgccgaagg gttcgtgcgc agcgcggggc tcgctcgagc tccacgccaa actgaaggcg
      781 ctcctccagg agcgcgggct cgcggagttc gccgtgcgag cctgcacgac gagctgcctc
      841 gatgcctgct ggacgggtcc aaccattgcc gtcgagccgg atcactactt ctatggccgc
      901 gtccgggaca gtgacctcgg tgacatcgtc gacgcgctcg cgaccggaac ccgcgtcgaa
      961 cggctggtcc tgaaacccga ggattatctc gagccacgcg agcgaaaaaa gctcagctga
     1021 gcaacgttct ggcccagaac agccaaatct gtgcaaaagg tgcatgaagc cgcggtcgat
     1081 gcaaagatcg cggtcgccac catgtcgttg ctcgagccac cccctacggt ccctttggat
     1141 tccattctgc ccgcggagcc gctgctcctg atgggcgccg ggccggtgcc gattccgcac
     1201 gcggtggcac gcgccaacgg ggtcgtgatc aatcatttgg gcgagacgat ggatcgggtg
     1261 atccggagcc tgaaggtgat ggcgcgctac gccttccaga cccgaagtga gaggatcctc
     1321 ggcgtcgccg ggcccgcctc ggcggcgatg gaaatggcgg tgtcgaactt gctgtggccc
     1381 ggccgtagag cgctttgcct caagaccgga actttcagcg gacggctcgg cgaaatggcg
     1441 acgggcgtag gcgccgacgt acgcacaatc accaccgata ccgcgaagcc catcctcgcc
     1501 gaacgggtcg cgcgcgcgtt ggccgaagag cacttcgacg tcgtgatgct ggcgcagggc
     1561 gagacctcgt gcggcgtgtt gaacgcagag ctgcccgcca tcgcaaagct ctgccgggac
     1621 catggcgcgc tcttgatcgt cgacgcggtg gtcaccctga gcacgatgcc ggtcgagatg
     1681 gacggttggg gactcgacgc cgtcgtcacg ggcggccaaa agggcttgtc gtcgattccc
     1741 ggcgtgtcgc tcctcgcctt ttcggaacga gcctgggaag ccatcgaacg gcgtaccgcg
     1801 cccatgccgc actggtgctt cgacgcccgg cgcgcggagc gcttttgggc acaccaacaa
     1861 tatcactaca ccgcgcccgt ccccggcgtg ctcgcgctgt acgaggccct gcggctcgtc
     1921 tgcgaagagg gcctcgaagc ccgcttcgag cgccaccgaa cgagctccat ctcactccag
     1981 gccgggctct ccgcgatggg cctcgagctc ttcgtcccgg ccgagcatcg gctcaactcc
     2041 gtggtcgcga taggcgtccc gcggggcgtc gacgcaccgc aggttttgtc cttcatgtcg
     2101 cgcaaattcc gggtcgaaat ctcgggcgcg ttcgggctcg agatcg
//
LOCUS       DASTKS010000375         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_282711, whole genome shotgun sequence.
ACCESSION   DASTKS010000375 DASTKS010000000
VERSION     DASTKS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282711"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VFQ35_07785"
     CDS             complement(<1..1697)
                     /locus_tag="VFQ35_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9930571.1"
                     /translation="MSEAGPSVLPTAVRAAPQLHQRARRFRRLLVGAILLAPSLLVIG
                     LDVMRRGERLRTLSGPYVLTYAAAIIEGGLLWATLIYAASRRRGRLRWLNAVLFVIAL
                     TFTVGGQTYFFGQYNAYLNVDVSVFASNFKDSVLNQLFADIGNYLRAKLPPLALSLLL
                     VWAARRWVRPRRRRALVASLVAPALLVGSFFIPTQHRQTQASTPDVLYLNAVGGLLRT
                     QLGMTEQSNQLRPRARNSRPVPALTRSPSLPKRNVFLVILESVRSDSVCIEYSPDCKK
                     TESTNLLFPARFPLTNMRSMDSCTAVSLAVLWSGLLPTESRDTLHTWPLIFDYAKAAG
                     YDTAFWTSQNMMFGNARLWVKNLGVRSFVSATDLDPTADLDMGAPEIYLAERLKNEVR
                     GLKEPFLAVVQLSNVHYPYFVDANQPMPFEPWTTSKGPDENRAFKNYYQNSVYQQDRH
                     VAEFLRALRSTPAGEHAVIVYTSDHGEAFREHGQMGHTFSVFDEEIHVPAWVDAPPGT
                     LTEAEERALRDKRDAYVFHVDVAPTILDLIGIADDPQIAEYRQKLQGHSLLRPELTD"
     gene            1864..>2506
                     /locus_tag="VFQ35_07790"
     CDS             1864..>2506
                     /locus_tag="VFQ35_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9930572.1"
                     /translation="MRRFGFALLLISTLVAGCNRGAQATAEARPARPKPAAKAAKKAE
                     PKPAPEPAEHAEGPRKFVVPFAWEASRDEPLAQARSYLKEILGDNRVYMEHGPKFFAQ
                     FADGQKPRATVVTCADSRVHTPAFDATPENDDFMVRNIGNQIENGLGSVEYGVDHLET
                     PVLMIMGHTGCGAVKAALGDISTQSEAVRKELEKLHIGKPKGDAKSDAAWSEAV"
BASE COUNT          472 a          769 c          866 g          399 t
ORIGIN      
        1 tggtcggtga gctcggggcg aagcaagctg tggccttgga gcttttggcg gtactcggcg
       61 atctgcgggt cgtcagcgat gccgatgaga tccaagatcg tcggcgccac gtcgacgtga
      121 aacacgtagg cgtcgcgctt gtcgcgaaga gcgcgctcct cggcttcggt caaggttccg
      181 ggcggcgcat cgacccaagc cggcacgtgg atctcttcgt cgaagacgct gaaggtgtgg
      241 cccatctgtc cgtgttcgcg gaacgcctcg ccgtggtcgc tcgtgtaaac gatgaccgcg
      301 tgctcgccgg ccggcgtgga gcgcagtgcg cgcaggaact cggcgacgtg acggtcctgc
      361 tggtaaacgg agttctggta gtagttcttg aaggcgcggt tttcgtccgg tcccttgctc
      421 gtcgtccacg gctcgaacgg catcggctgg ttcgcgtcga cgaaataggg atagtggacg
      481 ttggagagct ggactaccgc gaggaacggc tctttgagcc ctcgcacctc gttcttgagc
      541 ctttctgcga ggtagatctc gggcgcgccc atgtcgaggt cggcggtcgg atccaaatcg
      601 gttgcgctca cgaagctgcg aacgccgagg tttttcaccc acaagcgcgc gttgccgaac
      661 atcatgttct ggctcgtcca gaaggcggtg tcgtagccgg cggctttggc gtagtcgaag
      721 atgaggggcc aggtgtgcag cgtgtcgcgc gattcggtcg gcaagagccc cgaccacagc
      781 accgcgagcg aaaccgccgt gcacgagtcc atcgagcgca tgttcgtgag cggaaagcgt
      841 gccggaaaca gcaggttggt ggactcggtc ttcttgcagt ccggcgagta ttcgatgcac
      901 acgctgtcgg agcgcacgct ctcgaggatc accaagaaga cgttgcgctt cggcaacgaa
      961 ggcgagcgag tcagggccgg caccggccgc gaattgcgcg cccgcggtcg cagctggttc
     1021 gactgctcgg tcataccgag ctgcgttcgg agcaacccgc caaccgcgtt caggtacaga
     1081 acgtccggcg tcgaggcttg cgtttgacgg tgctgagtgg ggatgaagaa gctgcccacg
     1141 aggagcgccg gcgccacgag cgaagcgacg agcgcgcggc gtcggcgtgg ccgcacccag
     1201 cgtcgcgcgg cccaaaccag cagcaacgag agcgcgagag gaggcaactt cgcgcgcaag
     1261 tagttgccga tgtccgcgaa cagctggttc aaaacgctgt ctttgaagtt cgacgcgaac
     1321 acggacacgt cgacattcag gtaagcgttg tattgcccga agaagtaggt ctgcccgccg
     1381 acggtgaagg tcagcgcgat cacgaacagg acggcgttca gccagcggag ccggccgcgg
     1441 cgacgcgacg cggcgtagat gagcgtcgcc cacagcagcc caccctcgat gatagcggct
     1501 gcataggtga ggacgtaggg cccggagagc gtgcgcaggc gttcgccgcg ccgcatcacg
     1561 tcgagcccga tgaccagcaa tgatggcgcg agcagaatcg cgccgacgag aagacggcgg
     1621 aaacggcgcg cccgctggtg cagctgcggc gcggcgcgaa cggccgttgg gagaacggag
     1681 ggacctgcct cggacaacgg gcggagttta ctcggagaac ggagtctgca aaggggcttc
     1741 cgttgacgag cacgcttcgg acgcctcgcg cgccgtgtaa ccgggcgcct ctctgccggc
     1801 gctggcgcgg caaccgtcgc atggactcaa gctgggacag ccggagccgt attgaactag
     1861 cgcatgcgcc ggttcggctt cgccctgctc ctcatttcca cactcgtcgc gggttgtaac
     1921 cgcggcgcgc aggcgaccgc cgaggctcgt ccagcgcggc cgaagcccgc cgccaaagcg
     1981 gcgaaaaagg ccgagccaaa gcccgcgcca gagcctgccg agcacgcgga agggcctcga
     2041 aaattcgtcg tgccgttcgc ctgggaggct tcgcgcgacg agccgctcgc tcaggcgcgc
     2101 tcgtacctga aggaaatcct cggcgataac cgagtctaca tggagcacgg accgaagttc
     2161 ttcgcgcagt tcgcggacgg acagaagccg cgcgcgaccg tggtcacctg cgccgattca
     2221 cgcgtgcaca cgcccgcctt cgatgcgacg cccgagaacg acgacttcat ggttcgcaat
     2281 atcggcaacc aaatcgaaaa cgggctcggc tcggtcgaat acggcgtcga ccacctcgag
     2341 acaccggtgc tgatgatcat gggtcacacc ggctgcggtg ccgtcaaggc tgcgctgggc
     2401 gacatctcga cccagagcga agcggtgcgc aaggagctcg agaagctcca catcggcaag
     2461 cccaaaggcg acgcaaagag cgacgcggct tggagcgagg ccgtcg
//
LOCUS       DASTKS010000376         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1400045, whole genome shotgun sequence.
ACCESSION   DASTKS010000376 DASTKS010000000
VERSION     DASTKS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400045"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..862)
                     /locus_tag="VFQ35_07795"
     CDS             complement(125..862)
                     /locus_tag="VFQ35_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9930573.1"
                     /translation="MITLENVSKSFSGRPALSEVDLKVPRGCLYGLIGPGAAGKTLVL
                     KAICGLITPDSGRVVCGGNDVGRLSPAELSAYRRSIGMQFQNNALFDFMTVAENIAFP
                     LRRLMNLSDDEVRLRVAERLTRVQLPGFEDRSPSGLSGGQKRRIGIARATVTRPPIVL
                     CDEPAAGLDPVTSLRIFELLRAEQRAANSTVILVSSDVDRLLTVTDRIGMMHEGRLVF
                     DGTTAEARESTNPLIRQFLRGALTGPL"
     gene            complement(859..1599)
                     /locus_tag="VFQ35_07800"
     CDS             complement(859..1599)
                     /locus_tag="VFQ35_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930574.1"
                     /translation="MPVAANLEEADANQAVTMLEQSSLSATKERDPEHEGRYRVSVLN
                     ADAANAIGLLSRENLPPRSSPGVLEALGQGGVIPSRLAEQARWTAGVAGDLERSLRTL
                     DGVLSARVHLAIPVKEPLEGETAAQKPSASVLIRHRNVTPPIAASEVQRLVAGAVTGL
                     SPEQVNVVMTPSPASRMVASDAVHLGPLSVSRGAGSVLRASGALVVLLNLVLLAGVFY
                     LWTRLRRSEGALLEARAKLEPTAATKRK"
     gene            complement(1769..3298)
                     /locus_tag="VFQ35_07805"
     CDS             complement(1769..3298)
                     /locus_tag="VFQ35_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930575.1"
                     /translation="MTSSQQHLITPIPVGTGVVPGTLRARVEELRARGTVMNLKQAVG
                     ICVPLCVQAAEMHASGALLYLHPSSLVEDEYGFFQISPQLAHNAPLLPRDKACLAPEE
                     RSGRPGSARASVFAVGAILYELVTGQAVGPAMRRPSELAAGIPQAFESVLAKALVSDP
                     THRPDDLLALAQALHQFAPPGTVPPPPPADTSHLDHDEHFDVDVSMSMMPPEPSPAAS
                     SARQPAQPARPARVDATTELASLKARLESDPRPRYVVVKDGMDHGPFNAVELLQQIAS
                     HTFTEGDVVRDALSKDERAIKDWEEFAPFAEHAKLHREIKAEKAAIDRVVVQEQRSTR
                     GKAFIGILLVGSALVAAAVWFLTQRGKRNDEIAVQGDTASNVEVDAGLSGSKRPGGGG
                     RVVGRSGGVPILGGGMSCEAAQAAYVEEMNVGGPRGQADITAGQYGSILNRGSYFGHC
                     GVPDSMKLNICAAVQNGRAVGVSVVSSPRDRRVESCVSASVRGLSFPSHPKLDVTRTQ
                     F"
BASE COUNT          616 a         1228 c         1281 g          646 t
ORIGIN      
        1 gctcctcgaa aggcttcagc cttcctccgg tgctcactcc tcgggcgcgc ttctcgctcg
       61 cgtttcttgg ctctcgcgac gggcaggaac ttgggacacc ccctagctcg gactccgagc
      121 cttttcagag tggcccggtc aaagctccgc gcaaaaactg gcgaatcagc gggttcgtgc
      181 tctctcgggc ttccgcggtg gtgccgtcga agacgagtcg tccttcgtgc atcatgccga
      241 tgcgatccgt aaccgtgagt aggcggtcca cgtcgctcga gacgaggatg acggtgctgt
      301 ttgcggcgcg ttgctctgcg cgcaacagct cgaagatgcg caggctcgtg accgggtcga
      361 ggccggctgc cggttcgtcg caaaggacga tgggagggcg ggtcacggtg gcacgagcaa
      421 tgccgattcg ccgcttttgg ccgccgctca ggccgctcgg agagcgatct tcgaaacccg
      481 gaagctgcac tcgggtgagt cgctcggcga ctcgcaggcg cacttcgtca tcggacagat
      541 tcatgagccg gcgcagcggg aacgcgatgt tctctgccac cgtcatgaaa tcgaagagag
      601 cgttgttttg aaactgcatc ccgatgcttc gtcggtacgc cgagagctcg gcgggcgaga
      661 gtcgcccgac gtcgttgccg ccacacacga cacgtcccga gtccggcgtg atgagcccgc
      721 aaatcgcctt caagaccagc gtcttgccgg ccgctccggg gccgatcagg ccgtaaaggc
      781 agccgcgcgg caccttcagg tcgacttccg agagagccgg gcgccccgaa aagctcttcg
      841 atacgttctc gagcgtgatc acttgcgctt cgttgcggcg gtcggttcga gcttggcgcg
      901 cgcttcgagc agggcgcctt cgctccgacg cagccgagtc cacaggtaga agacaccggc
      961 gagcaagacc aagttcaaga ggacgacgag cgccccggag gcgcgcagga ccgagcccgc
     1021 cccgcgactc acgctgagcg gaccgaggtg cacggcatcg ctcgctacca tgcgcgacgc
     1081 ggggctcggc gtcatgacga cgttcacctg ttcgggcgaa aggcccgtga cggcgccggc
     1141 gacgaggcgc tgcacctcgc tcgccgcgat cggcggagtc acgttgcgat gccgaatcaa
     1201 aaccgaggcg ctcggctttt gcgctgcggt ctcgccctcg agcggctctt tcacgggtat
     1261 ggccaggtgg acgcgagcgc tgaggacgcc gtcgagcgtg cgaagcgagc gctccaagtc
     1321 tccggccacg cctgcggtcc agcgcgcctg ctcggccaag cggctcggga taacgccgcc
     1381 ttgccccagc gcctccagca ctcccggact cgagcgtggt ggcaaattct cccgggagag
     1441 aaggccgatg gcgttggcgg cgtcggcgtt gagtaccgag acgcgatagc ggccctcgtg
     1501 ctcggggtcg cgctccttcg tcgccgacaa gctcgattgt tcgagcatgg tgacggcctg
     1561 gttcgcgtca gcttcctcga ggtttgcagc gacgggcacg ctgcacccac tcaatgcaag
     1621 gcatatacaa agccacgctc cgccgcgcat gggcgcacca tacctcaacc cgcgcggcgg
     1681 gctaaggccc gcgaatgggt agtcacaccg cgcgagccgt ttccacgggc cgtccgaaat
     1741 gccttccggc ttggagtcgg ggcttgctct agaactgcgt ccgggtgacg tcgagcttcg
     1801 ggtggctcgg gaagctcaag cctcgcaccg acgccgagac gcacgattcg acgcgacggt
     1861 cacgcgggct cgaaacgacg ctcacgccga cggcacggcc attctggacc gccgcgcaga
     1921 tgttgagctt catcgaatcg ggaacgccgc aatggccgaa atacgagccg cgattcaaga
     1981 tcgagccgta ctggcccgcg gtgatgtctg cctgccccct cggcccaccg acgttcatct
     2041 cttcgacgta ggcggcttgg gcggcttcac agctcatacc gcctccgagg atgggcacgc
     2101 cgcccgagcg ccccacgacg cgtccaccgc cgcctggccg cttcgagccg gagaggccgg
     2161 cgtcgacctc gacgttcgat gccgtgtcgc cttgcaccgc gatctcgtcg tttcgctttc
     2221 cacgctgcgt gagaaaccag accgcggccg cgacgagcgc gctgccgacc agcaaaatcc
     2281 cgatgaacgc cttgccgcgc gtgcttcgtt gctcttggac gacgacgcgg tcgatcgcgg
     2341 ccttctccgc cttgatttcg cggtgcagct tggcgtgctc ggcaaaaggc gcgaactctt
     2401 cccagtcctt gatcgcgcgc tcgtctttgc tgagcgcgtc gcgcacgacg tcgccctccg
     2461 tgaacgtgtg gctcgcgatc tgctgcagga gctccaccgc gttgaagggg ccatggtcca
     2521 tgccgtcctt cacgaccacg tagcgaggcc gaggatcgct ttcgagccgc gctttgagcg
     2581 aagcgagctc cgtcgtcgca tcgacgcgag ctgggcgtgc cggttgcgct ggctggcgcg
     2641 ccgaggaagc cgccggtgag ggctcgggcg gcatcatcga catcgagacg tccacgtcga
     2701 agtgctcgtc gtgatccaga tgcgacgtgt cggcgggagg cggcggaggg acggtgccgg
     2761 ggggcgcgaa ctggtgcagc gcttgtgcga gagccaggag gtcgtccggg cgatgcgtcg
     2821 ggtcggaaac cagcgccttg gcgagcacgg actcgaaagc ctgcggaatg ccggccgcga
     2881 gctcgcttgg ccgccgcatg gcgggaccca ccgcctgacc ggtgactagc tcgtagagga
     2941 tcgctccaac ggcgaacacg ctggctcgcg cgctgccggg gcggccgctg cgctcctccg
     3001 gggcgaggca cgccttgtcg cgcggcaaga ggggggcgtt gtgagcgagc tgcggggaga
     3061 tctgaaagaa gccgtactcg tcttcgacga ggctcgaagg gtgcaggtag agaagggcgc
     3121 cgctcgcgtg catttcggcg gcttgaacgc agagcggcac gcaaatgccc accgcctgct
     3181 tcaggttcat gacggtgccc cgcgcgcgaa gctcctcgac gcgggcgcgc aaggtgcctg
     3241 gaacgacccc agttccaacg gggatgggcg tgattaggtg ctgttgcgag ctcgtcatcc
     3301 ggggggcgga tactagcaaa gttcaggttg ctgcggtgaa ggggtcccga cggtcaccac
     3361 ctcggccgct gcgtctgaac tcattgggtg ggaccggttt cggacagggc cgcgcggcct
     3421 gcgccggcaa actcgaagat cgggtcaaca aagttcaacc cacgttcaac cttgtaggca
     3481 gctcggcgat gttcaccgat gagcggttcc ttaaccagct ttacgaatgc gtgtggcgca
     3541 gggcgcgggc ggtgctcgga agagttgctg aacccagtac ggtcgctgcc ggcggtggct
     3601 aaagggcggt ttgatccgag cgaacttcgc tctggtagtg acgacggcgc actcggtact
     3661 tgccttcgat gaacggtccc agcacgggcg cgatgttctg cggaacgtcg gcgtcccatt
     3721 caccgccgtc cacgtagtcg gtcacgcgct ggcccgcccc caatagagcg a
//
LOCUS       DASTKS010000377         7496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_729348, whole genome shotgun sequence.
ACCESSION   DASTKS010000377 DASTKS010000000
VERSION     DASTKS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729348"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFQ35_07810"
     CDS             complement(<1..413)
                     /locus_tag="VFQ35_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930576.1"
                     /translation="MRCGCVLLSGVFFACGTSGGSSGGSGGATSGGATSASGGAALGG
                     SASGGAASGGTNSSGGAMSGGSTSRGGASSGGASSGGAASGGASSGGAASTSGGSSNA
                     GAGGNASGGTPSTNLPSLKVEGPLLKDSTGKTIILR"
     gene            complement(855..1562)
                     /locus_tag="VFQ35_07815"
     CDS             complement(855..1562)
                     /locus_tag="VFQ35_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9930577.1"
                     /translation="MRRTSESVTPASDLLAERTPWAAHLDCEQVIETLEPWVTPERRA
                     RLMGVLDARVSSVTVLMDAPHDPHNAAAVVRTCEAFGLPEIHIVPRAEEPLMARSVSR
                     GAEHWVDVNIHPSPHVAIDALRSQGFTLVATHPAGELSKEDLAKVPRVALLLGNEHDG
                     LREELTRAADHSVRIPMRGFVESLNVSVAAAILLSAAAEHRPGDLSVERKRALYARGL
                     YRTLPRAADFLRARYGS"
     gene            complement(1623..1778)
                     /locus_tag="VFQ35_07820"
     CDS             complement(1623..1778)
                     /locus_tag="VFQ35_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930578.1"
                     /translation="MPFAEPPVSIPVTPENIEEVSPYKIMLLPRLRKVGLPRYPAALV
                     RLCTDSP"
     gene            1794..2117
                     /gene="trxA"
                     /locus_tag="VFQ35_07825"
     CDS             1794..2117
                     /gene="trxA"
                     /locus_tag="VFQ35_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9930579.1"
                     /translation="MASKDVQVFNDLNFETEVLGSKVPVLVDFTATWCGPCKQLSPIV
                     DQLATELAGQVKVGKLDVDDSPVTAGKYGVRGVPTVMVFKDGERAAQHVGLTTKQKLI
                     DLVKV"
     gene            2222..5314
                     /locus_tag="VFQ35_07830"
     CDS             2222..5314
                     /locus_tag="VFQ35_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930580.1"
                     /translation="MPLVAEAALAAAYASPARFVYHPRERARPRAFRKLSDGDQLLVG
                     ERGERWQYSPKNRWLRPGAILAPEPLIAVLGETSAFSFIGASGTAYVARDPLGPFERS
                     SAPVEALTSVSAAGTSIIGVRQTRSLLRSGDFGVSWQNVGPRGESVVSVGIEASGLGY
                     ALVTPEAVYETHDFGDTWQKLDAASVGALELVTAFDGKVRALTPLGERAFDRGEKRWR
                     SALPVAPPSATWPSPPRGPDAAALGEGRAVIVNGSYYELVAATERGTWDTVSGPLGGP
                     YQRKPFLPAKGCKAVRVAAFEQWLVFACFKEAAESLSQPIELHRGGFTAERFERLPGR
                     LDGTLSAFRLSVGASGHWIASGVCPPGVAGTGCAPSGIQRERSPDEGLASGDETTKKR
                     ALNSAGALAAAPSLSNVALALAFSTDGRSAYAVGRRSKTGRFALFVSRDAGRTFAPRD
                     LDLGQVASDDSDDDMVERSPGTRVDSLSAAEDGAVAIAFSHYGRRTLVVTDDQGKLLS
                     ASEPPEERALIGASGLRAIAIAHKSRQVWESLDGGVTFEPAGTLPLELCPNEEGCDVP
                     VRCAPSGCVIGRELTRVGWGGISADEAVILPPPLRPLRQLAERRVSTPISCALDSGRF
                     TPLPGVGDAPRAHDSQIGKAAWFVVADDPAHAAATIFVGRKGRVEPQSLLEPASHPEQ
                     RAYAVLSQVEGVAAVRYRLPEAQPGKASLTDVEIAAQNFIEGQTVRARIADGGAYSPG
                     DYTTSSSHTPQAQPDLVSIASGGVYLRLHQSARADQPTFFLDGRHVETIPAVEWPVDS
                     RFPARAEMVRIGAANVPILFVGRGAAIARARRTGAAWDFEAFATGMVDPASFGLVSFD
                     NIAYVGNRAGLYLETQDGTGTIASAQIYPFRPDGALVDPPVLVPTQLGLGEHPTACDA
                     ERRAHTPRIVAAYQPGTRHPIVVNDNVEGPRTLLTAFAVLHGTPEAPCVASYEAVGLS
                     ADTGQATRETALILMDDLEHSVLFRRGAGDAGQVSYRHMSCRFDPAVEVPPEVYRALP
                     "
     gene            complement(5380..6426)
                     /locus_tag="VFQ35_07835"
     CDS             complement(5380..6426)
                     /locus_tag="VFQ35_07835"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HET9930581.1"
                     /translation="MAGEIVLVSRGARSREEDQASHLLRFPSHWRAAPVVNPAEMRIE
                     RETLEWLAAHGMGTSPAEREKLRKFECGKYGGYSLPRAPLPEALLVTQFISLWLFWDD
                     VEVEDHTDWSIDDVAAAFTAGPSPSTNSYVMAWNDVASRLRARGSARWLARLAESMRE
                     WLGNAQHETKLGMALRKRAEWPSLETLFECRTVSIGMYPTFYLIEIAEHLELPDEFHE
                     HDLVKDLKRTASRLVGIGNDLGGLAKDLKSGWLNLVVALAHGASIRIDEAFGRLVEIH
                     NREVLDFDRAAARLPSFGEKLDPLVQTWLEAVRYSVYGFALWESCAERYQEYTALVEG
                     RPLRAMLTIDASAV"
     gene            complement(6532..7398)
                     /locus_tag="VFQ35_07840"
     CDS             complement(6532..7398)
                     /locus_tag="VFQ35_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9930582.1"
                     /translation="MPIAGDVRYGYPGRCMDSVERQIGLITEVALGAPDLRSFELAWI
                     ELLRPSIGFETACSIWTDHTGASLGAAALGYPEATLRGRFSAYMSELSRREVAAFSSA
                     RPIVDTDLMSLRRRQELTVYRELLIPANVSCFVTNVWHSRWGVFGVHLARCGVTRTFR
                     ARDLDRLGRVLGSVKLGQALLAAEELRVSADVEWWGGVWGLSPKELEVARLVARGFHN
                     PEIARLLRSSRYTVRNHLVSVFRKAAVSTRTELVFAMNAPRALAANFVRHRGSSSRAW
                     SGFLASARAKVG"
BASE COUNT         1243 a         2538 c         2415 g         1300 t
ORIGIN      
        1 cgcaaaatga tggtcttgcc ggtggagtct tttaggagcg gcccctcgac cttgagggaa
       61 gggaggttgg tgctcggagt gcctccgctc gcattaccgc cagcacccgc gttgctcgag
      121 ccaccgctgg tcgacgccgc gccgccactg ctcgctccgc ccgacgccgc gcctccactg
      181 ctcgcgccgc cactgctcgc tccgccgcgg ctcgtgctgc cgccagacat tgcgccaccg
      241 ctcgagtttg ttccgcccga cgcggcaccg ccgctcgcgc tgccacccag cgcggcaccg
      301 ccgctcgccg aagtcgctcc gccgctggtc gcaccgccgc taccacccga gctaccgccc
      361 gatgtcccgc acgcaaagaa cacaccgctc agcagaacgc acccgcaacg catcgctcgc
      421 atatcgagtc ctttcaggct tcgaagttct tccgaagctg tttactcgca ttctatggaa
      481 gcggtagcgc cgagacgaaa attcgtagtg tcgtcagcgc cacgtgcgcg ccgccgtctc
      541 cgccggctct tgtcaggttc gggtcacatt ctttcttgcg cgaccgctga ttgaaaccgg
      601 tgtggacgat aaccgtcgta ctgctcgaga ggtcgaatca cacatctctc ggaggctttg
      661 cttgcgagct cgtcgagtca aaacgctcgc ttcgagccaa aatgcaaacc agttccgttg
      721 gctcgggtcc gcgatgtgcg cgaaccgagc ggatcgggcg gcaccctgcg atcggcgact
      781 acgcccgccg gtcgccgcca gcggtgacgg tgaagtcgtc gaagtctggc tctcggaccc
      841 gaaggcggag tcgttcagct gccgtagcgc gcccgcagga aatctgccgc gcgcggcaga
      901 gtccgatata ggccgcgggc gtaaagagcc cgcttccgct cgacgctcaa gtcgccgggg
      961 cggtgctccg ccgctgcgct caagagaatc gcggccgcga cgctgacgtt caggctttcg
     1021 acgaagccgc gcatcgggat gcgcacgctg tggtccgcgg cgcgcgtgag ctcttcgcgc
     1081 aaaccatcgt gctcgttgcc gagcaacagc gcgactcgcg gcaccttcgc gagatcttcc
     1141 ttgctgagct cgcccgccgg gtgcgtcgcg accagggtga acccctgtga acgcagcgca
     1201 tcgatggcga cgtgcgggct cgggtgaatg ttgacgtcga cccaatgctc cgcgccgcgt
     1261 gagaccgagc gcgccatcaa aggctcctcg gcgcgcggga cgatatgaat ctcgggcaac
     1321 ccaaacgctt cgcacgttct caccaccgcg gcggcgttgt gcgggtcatg cggagcatcc
     1381 atcaacaccg tcacgctgct cacacgcgcg tcgagcaccc ccatgagccg agctcgtcgc
     1441 tccggcgtga cccagggttc gagcgtttcg atcacctgct cgcagtcgag gtgtgccgcc
     1501 cacggggtgc gctcggccaa caggtccgac gccggggtga cgctttcact ggtacgacgc
     1561 atcgctagat ccaagggtcg gcttccgggc gggacgcgct cgggtcggga gacgggagct
     1621 tttcatggcg agtcggtgca aagacggacg agagccgccg ggtagcgcgg gagccccacc
     1681 ttccgcaacc gaggaagcag cattatcttg tacggactga cctcctcgat gttttccggc
     1741 gttaccggaa tgctgactgg cggctcagcg aaaggcatgg cacggagcag cagatggcat
     1801 ccaaagatgt gcaggttttc aacgatttga acttcgagac agaggttctt ggttccaagg
     1861 ttccagtgct cgtggacttc accgccacgt ggtgcgggcc ctgcaagcag ctatctccca
     1921 tcgtcgacca gctcgcgact gagcttgcgg gtcaggtgaa ggtgggcaag ctggacgtcg
     1981 acgattcgcc cgtcaccgcg ggtaagtacg gtgtgcgcgg cgttccgacc gtcatggtgt
     2041 tcaaggacgg cgagcgagcg gcgcagcacg ttgggctaac cacgaagcag aagctgatcg
     2101 acctcgtgaa ggtgtgacca caggcttgcg cgcgtcggcc gtggccgcgc tcgggttagg
     2161 gctcgcggcc tgtttcgggt gcgcgggccg tcctacccgg ccacctggtt tcgttccgcg
     2221 agtgcccctc gtcgctgagg cggcgctcgc ggccgcgtac gcttccccag ctcgcttcgt
     2281 ctatcatccg cgcgagcgag cgcggcctcg cgcttttcgg aagctctcgg atggcgacca
     2341 gctgctcgtc ggagagcggg gtgagcgctg gcaatactct ccaaagaacc gctggctgcg
     2401 gcccggagca atcttggccc cggagcccct catcgcggtg ctcggcgaga cctccgcgtt
     2461 ctccttcatc ggcgcgagcg gtaccgctta tgtcgctcgc gatccgctcg gccctttcga
     2521 gcgctccagc gccccggtcg aggcgttgac ctcggtgagc gccgcgggca cctccatcat
     2581 cggcgtccgg cagactcgtt cgttgctgcg cagcggtgac ttcggcgtca gctggcaaaa
     2641 cgttgggcct cgcggggaat cggtggtgag cgtcggcatc gaagcgtctg gcctcggtta
     2701 tgcgctcgtc acgcccgaag cggtctacga gacgcacgac tttggcgaca cttggcagaa
     2761 gctcgacgcc gcttccgtcg gcgcgctcga gctcgtgacc gccttcgatg gcaaggttcg
     2821 cgcgctgacg ccgctcggcg aacgcgcctt cgaccgcggc gaaaagcgct ggcgttcggc
     2881 gttgcccgtc gctccaccga gcgccacttg gccttccccc ccgcgtggtc ccgacgccgc
     2941 tgcgctcggt gaagggcgag cggtcatcgt aaacggctcg tattacgaac tcgtcgcggc
     3001 gaccgaacgc ggtacctggg acaccgtttc ggggccgctc ggtggtccct atcagcgcaa
     3061 accatttctg cccgcgaaag gttgtaaagc agtccgagtc gcggccttcg agcagtggct
     3121 cgtcttcgct tgcttcaaag aagcggcgga gtcgctctct caaccgatcg agcttcatcg
     3181 cggcggcttc acggctgagc ggttcgagcg actacccggg cgcctcgacg gaacactttc
     3241 ggcatttcgg ctgagcgttg gcgcgtccgg tcactggatt gcgagcgggg tctgtccgcc
     3301 gggcgtagcc ggcaccggct gcgcgccgag cggtatccag cgtgagcgga gccctgatga
     3361 aggcctcgca agcggagacg aaacgaccaa aaagcgcgcg ctcaattcgg cgggcgcact
     3421 cgccgcggcg ccgtcgctat cgaacgtcgc gctcgctctg gccttctcga ccgacggccg
     3481 cagcgcgtac gcggtgggac ggcgcagcaa gacggggcgc ttcgcgttgt tcgtctcgcg
     3541 cgacgctggc aggacgtttg ccccgagaga tctcgacttg ggtcaagtcg cttccgacga
     3601 cagcgacgac gacatggtcg agcgctcccc aggcacgcgc gtcgactcgc tcagcgcggc
     3661 cgaggacggc gcagtcgcga tcgcgttttc gcactacggc cgacgcacgc tggtcgtaac
     3721 cgacgaccag ggcaagctcc tctctgcttc cgagccgccc gaagagcgcg cgctcatcgg
     3781 cgcgagtggc ctgcgcgcca ttgccatcgc tcacaagtcc cgacaggtgt gggagtcact
     3841 cgacggcggt gtgacgttcg agccggctgg caccctgccg ctcgagcttt gcccaaacga
     3901 ggaggggtgc gatgttcccg ttcgctgcgc accatctggg tgcgtcatcg ggcgcgaact
     3961 cacgcgcgtc ggctggggtg ggatctccgc tgacgaggcg gtgatcttac cgcctccgtt
     4021 acgaccgctg cgccagctcg ccgagcgtcg cgtcagcacg ccgatttcct gcgcgctcga
     4081 ctcgggccgc ttcacgccgc ttcctggggt cggcgacgcc ccgcgcgcgc acgactcgca
     4141 aatcggcaaa gccgcgtggt tcgtcgtcgc cgacgaccct gcgcacgccg ccgcaaccat
     4201 atttgtcggg cgtaaaggcc gggtcgagcc gcagtcgttg ctggagccgg cttcccaccc
     4261 cgagcagcgc gcctacgcgg tgttgagcca ggtcgaaggc gtcgcggcgg ttcgctatcg
     4321 actgcccgaa gcacaaccgg gcaaagcatc gctcaccgac gtggagatcg cggcgcagaa
     4381 cttcatcgaa ggccagaccg tgcgcgctcg catcgccgac ggcggagcgt attcacccgg
     4441 cgactacacg acgagcagct cgcacacgcc gcaggctcag cccgatctcg tcagcattgc
     4501 gtccggcggc gtttacctgc gactgcatca gagcgcgcgc gccgatcagc cgacgttctt
     4561 cctcgacggt cgccacgtcg agacgattcc cgcggtggag tggcccgtcg actctcgctt
     4621 tccggctcgc gccgaaatgg tgcggatcgg ggcggcaaac gtgccgatct tgttcgtcgg
     4681 tcgcggcgcc gcgatcgctc gagcgcgccg caccggcgcc gcttgggact tcgaagcctt
     4741 tgcgacgggc atggtggacc ccgcttcgtt cggcctcgtc tcgttcgaca acatcgccta
     4801 tgtcggcaac cgcgctggct tgtacctcga gacgcaggac ggcaccggca cgatcgcgag
     4861 cgctcaaatc tatccttttc gccccgacgg cgcgctcgtc gaccctcccg tgctcgtgcc
     4921 cacacagctc gggttgggcg agcatcccac cgcgtgcgac gccgaacgca gggcacacac
     4981 gccgcgcatc gtagccgcgt atcagcctgg cacccgtcac cccatcgtcg tgaacgacaa
     5041 cgtcgaaggg cctcgcactt tgctcaccgc cttcgcggtc ctgcacggca ctcccgaagc
     5101 tccgtgcgtc gcctcttacg aagcggttgg gctctccgcc gacaccggcc aagccacgcg
     5161 cgagaccgcg ctcattctca tggacgatct cgagcactcc gtgttgttcc gacgcggagc
     5221 gggcgacgcg gggcaggtga gctaccgtca catgagctgt cgcttcgacc ccgccgtcga
     5281 agtgccccct gaagtgtatc gggcgctgcc ttagctgggc gttgagaagg cccgcgtcac
     5341 gacagaccaa tgccagtctt tggcgaggcg cctcggaact cagactgcgc tggcgtcgat
     5401 cgtgagcatc gcacgtagcg gtcggccttc gacgagcgcc gtgtactcct gatagcgctc
     5461 ggcgcacgat tcccagagcg caaagccgta aacgctgtag cgcaccgcct cgagccaggt
     5521 ctgtacgagg ggatcgagct tctcaccaaa cgagggcaag cgcgcggccg cccgatcgaa
     5581 atcgagcacc tctcgattgt gaatctctac gaggcggccg aacgcttcgt cgatacgtat
     5641 gctcgcgccg tgggccagtg ccacgaccaa gttgagccaa ccgctcttca ggtcttttgc
     5701 caagccgccg agatcgttcc cgatgcccac taggcgcgag gccgtgcgct tcaagtcttt
     5761 gacgagatcg tgctcgtgga actcgtcggg cagctcgagg tgctcggcga tctcgatgag
     5821 ataaaaagtc gggtacatcc cgatgctcac ggtgcggcac tcgaacagcg tttcgagcga
     5881 tggccattcc gcgcgctttc gcagcgccat cccgagcttg gtctcgtgct gcgcgttgcc
     5941 gagccattca cgcatcgatt cggcgagccg cgcgagccag cgagcgctcc cgcgtgcacg
     6001 caaacgactc gccacgtcgt tccacgccat gacgtaggag ttcgtgcttg gtgaaggtcc
     6061 cgccgtaaat gccgcggcga cgtcgtcgat actccagtcc gtgtggtcct caacctcgac
     6121 gtcgtcccaa aagagccaca gcgagatgaa ttgagtgacg agcagggctt ccggcagcgg
     6181 ggcgcgtggc agcgagtatc cgccgtattt cccgcactcg aacttgcgaa gcttttcgcg
     6241 ttcggcgggg ctcgtcccca ttccgtgggc ggcgagccat tcgagcgttt cgcgctcgat
     6301 gcgcatctcg gcgggattga cgaccggagc ggcgcgccaa tgcgacggga aacgcagtaa
     6361 gtgggatgct tgatcttcct cgcgcgatcg cgcgccgcgc gacacgagca caatctcgcc
     6421 agccatgacg cctcgtttgg gcgttcgcgg gtgcacgacg agtgcgcttc cgtcgaacgt
     6481 cggtcgaggc taggctgagc gtccgtgcgg cagaatggta cagctgtact actagccaac
     6541 cttcgcgcgg gcgctcgcga ggaagccgct ccacgcccgg ctcgaagaac cacggtgtcg
     6601 aacgaagttg gcggcaagag cgcgcggtgc gttcatggcg aaaacgagct cggtgcgcgt
     6661 cgacaccgcg gccttccgaa acaccgagac cagatggttc ctaaccgtgt agcgcgagct
     6721 tcggagcagc ctggcgatct cgggattgtg aaacccgcga gcgaccaagc gcgcgacctc
     6781 gagctctttc ggtgaaagcc cccagacgcc accccaccat tcgacgtccg ccgaaacgcg
     6841 cagctcttct gccgcgagta atgcttgacc caatttcacg ctcccgagca cgcgacccag
     6901 tcggtcgagg tcacgcgccc tgaacgtacg agtgaccccg cagcgggcga ggtgaactcc
     6961 gaatacaccc cagcgcgagt gccagacatt ggtgacgaag caggacacgt tcgcaggaat
     7021 caagagctca cgatagacgg tgagctcctg ccgacggcga agcgacatca ggtcggtgtc
     7081 gacgataggg cgggcggagg agaacgcggc tacctcacgg cgggaaagct cgctcatgta
     7141 cgcggaaaag cgcccgcgca gcgtcgcttc cgggtaaccg agggccgccg caccgaggga
     7201 cgccccggta tgatcggtcc aaatggaaca agccgtttcg aacccgatac taggacgcag
     7261 cagctcgatc caagcgagct cgaatgagcg tagatcgggc gctccgagcg ccacctcggt
     7321 gatgagacca atttggcgct ccacgctgtc catgcatcga ccggggtagc cgtagcgaac
     7381 gtcgcccgca ataggcatcg tttcaactgc agccgcgctg tttggccggg ccgcatcacg
     7441 cctgggggtt ccgtgccgcg ctcccgctgg cgcaccgggc aagtcggtgg tcgaaa
//
LOCUS       DASTKS010000378         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_61508,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000378 DASTKS010000000
VERSION     DASTKS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61508"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFQ35_07845"
     CDS             complement(<1..703)
                     /locus_tag="VFQ35_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9930583.1"
                     /translation="MDATPRDAVVLGLVQSITYVLPVSSDGHVALMDLLFGLRAPTPL
                     MAVALRFAALVSTAFVLRYRFARLLREGVLAFRYPDRLWTTSGGRDAVVVVFASFPTA
                     LFGLFLREPVSAWARSPLMVGIGLLGTSAWLLSTLWLKPGKSEFPGLLGSILIGVAQG
                     LSILPGLSRSAGTIACALWLGVRPDRAFELSFLASLPPLLAVVVLDARALSREPIALT
                     AVAALLALLATAVALG"
     gene            complement(792..2726)
                     /locus_tag="VFQ35_07850"
     CDS             complement(792..2726)
                     /locus_tag="VFQ35_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930584.1"
                     /translation="MANVECVRCGNRTEIANEASEDLQPSAPVCAGCGAPLHGSANSE
                     RAALNGIFSAFEQSVHPAAGDGLPSEPSAVPEGIRAEPSAPAEGPRAQPSAAAEAFLP
                     EPSAPSEGLPTEAASAEPPSGVSPRSDQSGFAGDVTTPETPSAIRAASAFESLGASSD
                     RAPMSPEFAHEPSETWNRPPETWSNRAEAWAEPPETVRYLMIPSVAAFDEARAAEAVA
                     SFPDPPRSEHLGELASSSERVVESSPVAAPLPPPELEPLRASLVDSGWSNPLTAAEPF
                     VSPGAEFAIDASERADEASAQSAESAPPSSVLDGSARVSKGPPPPPAWAFSGNDDDDE
                     SPPVRVNQDAPDNVERAEHPSFADDDAVDSHSDIAFSLVVAAPAAHDKVDAAMGAPAS
                     SVPPASSRFPRLTSEAPVVSSLSAPPTSESPPPWGEPGSKRRSQVMMGLAGLAVVASF
                     FLGRSSVSGSARSERVSAAKAEAPRPVAVAPLVAQPQPQPAPSALAPAAANVPAPVSE
                     GVAASSLAPVPSAAPAALSTARPDPGTPLMVVNTTKGHPPVLSSTVPSGSLDLAAADD
                     AIVDAASRASACRRPEQPQKRATVTVTFTAAGRVASANIAGIKFNGVTLRNCMGSTFL
                     GARVPAFNGPSVTVRKTLKL"
     gene            3112..5610
                     /locus_tag="VFQ35_07855"
     CDS             3112..5610
                     /locus_tag="VFQ35_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9930585.1"
                     /translation="MLRSLSFASVLAFGLAAKQASAETFEVRRAADDVVVLTGELAPT
                     PTTNGQPSAKLADFSALTEPGEYYLEVASVGKSVAFRVGDDVYDGELAKLMLGFYGWR
                     SGIDIAFDFDGVHYQHSAGHLKDGLLDYIDGQVGVTKDGTGGWYDAGDYGKYLPTASE
                     SVGNMLAAWELFSDRLQKLELPYLPEHGSGIPDFLSELKWELDWMLKMQYDDASGRIH
                     HKLNSPQFPGFILPAADTSKRYFASYSTAATAEFVATLAKAARAFAPYDALTGGYSKV
                     LLDAAQRSYAYLLANPVDVQYDASVLAAGTYQKMGADDRLWAAAEMWETTGDPIALAD
                     VEQRIGARAMFVANFDWDNMTTFGLVTYLLSQREGRDPAIVERLLQNLTNLADGLVYG
                     AGQNAYGRDLDSYYWGSNGVIARSCVMLQTAYRVVAPKPEFLDVCAAQVGYLYGRNQY
                     NRSQVTGAGVDPPLHPHARISGSDDVDAPYPGLLVGGGQSASNWQDAQSNFQTNEIAI
                     NWNAALVYALAGFISDKGAAASPARGALPAGACQVRVNSVGYLPERSKVATVVSDCTN
                     AGPDFQCSVTDKTLSGDTSGALSIIDDLEDGDTRATAKRGRSGAWFSYDDGSAGTRTQ
                     AEIKSAGRAGSKNAICISGQGFTGWGGGMGVSLAGEGSSRMLYDASPYTGVSFWARGS
                     GMFRAMLVDKYSDPAASLCTSCYDHFQAPFTPGSEWKQYTFSWKDLKQLGFGEMKPNV
                     CAAGLYALQFQWSGSQPFELCLDDVAFTQAAGSSDSADSPSVSPRGGGCGCRIGVVSE
                     SPAFAELGVLALGLTAVMSRRRRAARASASRRGS"
     gene            complement(5546..>6048)
                     /locus_tag="VFQ35_07860"
     CDS             complement(5546..>6048)
                     /locus_tag="VFQ35_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930586.1"
                     /translation="MIEVLCWAAFLRTLTQSIPQLFHAAGRPELAAYDSLFTLPCFVG
                     FGWVLVRAFGSTLGANAVSFAWMATYLCTLSVLFLMVRGIMPVKLREFLGCLGGPLSL
                     MGVLWGAARVQHALYVRFLPSAFATALGLGLSLLVALAYVRFVLKIRVGRRSPARRDD
                     GDSSPP"
BASE COUNT         1062 a         2017 c         2067 g          902 t
ORIGIN      
        1 cgccgagcgc gaccgccgtc gcgagcaggg cgaggagggc cgcgactgcc gtgagggcga
       61 tcggctcacg cgacagcgcg cgggcgtcga gcacgaccac cgcgagcaat ggaggcaggg
      121 acgcaaggaa cgaaagctcg aatgcccgat ccggtcgcac cccgagccac agcgcgcacg
      181 cgatcgtccc ggcgctgcgc gacagccccg gcagtatcga gagcccttgc gcaacgccga
      241 tgaggatcga gccgagcaaa cctggaaact cgcttttccc cggcttgagc cacaaggtcg
      301 acaggagcca cgcgctcgtc ccgagcagtc cgataccgac catgagcggg gagcgcgccc
      361 acgcgctcac tggctcgcgc agaaaaagcc cgaagagcgc ggtaggaaag ctcgcgaaca
      421 cgacgaccac cgcgtcgcgc ccgcccgacg tggtccagag acggtccggg taccggaagg
      481 ccaacacgcc ttcgcgcaac agccgcgcga agcgataccg caagacgaac gcggtcgaga
      541 cgagcgcggc aaagcgcaac gcaaccgcca ttagcggcgt cggcgcgcgg agcccgaaca
      601 agagatccat cagcgcgacg tgcccgtcgc tcgaaacggg caacacgtag gtgatgctct
      661 gcaccagccc gagcaccacg gcgtcccgcg gtgtggcatc catagacgcc tcgcacagta
      721 gtgcctgccc gaaggcggaa caatcgttac ttgccggcgg agctttccgg atcaatcgcc
      781 cggcgcggcg ctcacagctt cagggtcttc cgaacggtga ccgacggccc gttgaaagcc
      841 ggaacacgcg cgcccaagaa cgtagagccc atgcaattgc gtagcgtgac cccgttgaac
      901 ttgatgccgg cgatgttggc gctcgcgacc ctgcccgccg ccgtgaaggt caccgtcacc
      961 gtggcgcgtt tctgaggctg ctcgggccga cgacaggccg aggcgcgcga tgccgcatcg
     1021 acgattgcgt cgtcggccgc cgccaggtcg agcgagccgc tcggaacggt ggagctgagg
     1081 accggcggat gccccttggt ggtgttgacg accatgagcg gggtgcctgg gtcgggcctg
     1141 gccgtagaaa gtgctgcggg cgcagccgac ggcaccgggg cgagcgagct cgcggcgacc
     1201 ccttcgctca cgggggccgg aacgttcgcc gccgctggcg cgagcgccga tggcgccggt
     1261 tgtggctgag gctgggcaac gagcggcgcg actgcgacgg gacgcggagc ttcggccttg
     1321 gcagcggaca cgcgctcgct acgagcggag ccggacacgg agctccgccc gaggaagaac
     1381 gaggccacga cggcgagccc agctaggccc atcatcactt gagagcggcg cttcgaaccg
     1441 ggctcgcccc aaggcggcgg gctctccgac gttggcggcg ccgacaagga gcttacgacg
     1501 ggcgcttcgc tcgtgagccg cggaaacctg ctcgacgcgg gcgggaccga gctcgccgga
     1561 gctcccatcg cggcgtcgac tttgtcatga gctgccggcg cagcgacgac gagcgaaaac
     1621 gcgatgtcgg agtgcgagtc gacggcgtcg tcatcggcga aagatgggtg ctccgctcgc
     1681 tccacattgt ccggcgcgtc ttggttgact cgaacgggcg gcgactcgtc atcgtcgtcg
     1741 ttgccagaga aggcccaagc cggaggaggg ggcggccctt tactgacgcg agccgagccg
     1801 tcgagcaccg aactaggtgg cgcggactcg gccgattggg ccgaagcttc gtcagctcgt
     1861 tcactcgcat cgatcgcgaa ctcggccccg ggcgaaacga acggctcggc agcggtcaac
     1921 ggattggacc agccggagtc gaccaggctc gcgcggagcg gctcgagctc agggggtggg
     1981 agcggggcgg ccacggggct cgactcgacg acgcgctcgg agctcgaagc gagctcgccc
     2041 aggtgctcag agcgcggcgg gtcggggaag gaggccactg cttcggccgc cctcgcttcg
     2101 tcgaacgctg ccacgctcgg gatcatcaaa tagcgaacgg tctcgggcgg ctcggcccaa
     2161 gcctcggctc ggttgctcca ggtctcggga ggccgattcc aagtttcgga cggctcgtgc
     2221 gcgaactcag gcgacatcgg agctcggtcg gacgaggcgc ccagcgactc gaacgcgctc
     2281 gcggcgcgga tcgcgctcgg tgtctccgga gtggtcacgt cgccggcaaa tccgctctgg
     2341 tcgctgcgcg gagagacgcc cgatgggggc tcggccgaag ccgcctcggt cggtaaccct
     2401 tcgctcggtg cggacggctc cgggagaaac gcctcagcag ccgcggacgg ctgcgccctg
     2461 gggccttcgg cgggcgccga tggttcggca cgaatgcctt cgggcaccgc cgatggttcg
     2521 ctcggcagcc cgtcacccgc cgctgggtgc acggactgct cgaatgcact gaagattccg
     2581 ttcagcgcgg ctcgttccga attggcggaa ccgtggagcg gcgcgccaca gccagcacat
     2641 accggcgccg agggctgcaa gtcctcggaa gcttcgttcg ctatctcggt gcggttgcca
     2701 caccgaacgc actcgacgtt cgccacgcgc tcctccgttc cttggcatgt tcccccggcc
     2761 gacttgggcg cgcaagcccg gcggactccg gcgcgccaag gcgccgcatc ggttcggagc
     2821 ggggctctcg ggccccagag gcgcgaaagt gctcgctgag gtcgcgacaa tcggcgcgag
     2881 cctgcactgg tcgctccacg cccatcgagt ttttttggtg gaagtattca gtgtcgggga
     2941 cccggcctcg cctcgcgcca tggccgcgcg aagaccgcct cgggtcgagc tcggcggccc
     3001 ccgcagggcg cgaggagcca tgtcccagtc ttcgacctcg aatttatgga accggttcca
     3061 gtcgctattt ggctgcgcgc gacgagctcg gctgctatca aggcgcacac catgttgcgc
     3121 tcgctttctt tcgcgtccgt gctcgccttt ggcctcgcgg caaagcaggc ttccgccgaa
     3181 acattcgagg tccgccgcgc cgcggacgac gtcgtggtgc tcacgggtga gctcgcccct
     3241 acccccacca cgaatggaca accatccgcc aagctcgcgg acttcagcgc gttgaccgag
     3301 cccggcgagt attatctcga ggtcgcttcg gtaggaaaga gcgtcgcgtt ccgagtcggc
     3361 gacgacgtgt acgacggcga gctcgccaag ctgatgctcg gcttttacgg ctggcgctcc
     3421 ggaatcgaca tcgcgttcga cttcgatggc gttcactacc agcactccgc cggtcacttg
     3481 aaggacggct tgctcgacta catcgacgga caggtgggcg tcacgaaaga cggcaccggc
     3541 ggttggtacg acgcgggcga ctacggcaag tacttaccga ccgcgtcgga gagcgtcggc
     3601 aacatgctcg cggcgtggga gctcttctcc gaccggctgc agaagctcga gcttccgtat
     3661 ctgccggagc atgggagcgg cattccggac ttcttgagcg agctcaagtg ggagctcgat
     3721 tggatgctca agatgcagta cgacgacgcg agcggacgca tccaccacaa gctcaattcg
     3781 ccgcagttcc ccggcttcat attgccggcc gccgacacgt cgaagcgcta tttcgcgagc
     3841 tatagcaccg cggccacggc cgagttcgtc gcgacgctgg ccaaagccgc gcgcgcgttc
     3901 gcgccgtacg acgcgctgac cggtggctac tcgaaggttc tgctcgacgc cgcgcagcgc
     3961 tcctacgctt acctgctcgc gaacccggtc gacgttcagt acgacgcctc ggtgttggcg
     4021 gccggtactt atcagaagat gggcgcagac gatcggctct gggccgccgc cgagatgtgg
     4081 gaaacgaccg gcgacccgat cgcgctcgcc gacgtcgagc agcgcatcgg cgcgagagcg
     4141 atgttcgtcg ccaatttcga ttgggacaac atgacgacgt tcgggctcgt cacgtatttg
     4201 ctctctcaac gcgaaggcag ggatcccgcg atcgtcgagc ggctcttgca gaacctcacg
     4261 aacctggccg acggcttggt gtacggcgcg gggcagaacg cttatgggcg cgacttggac
     4321 tcgtactact ggggctcgaa cggagtcatc gcgcggagct gcgtcatgct gcaaaccgcc
     4381 taccgcgtgg tcgcgcccaa gcccgagttc ctggacgttt gcgcggcgca ggttggctat
     4441 ctttacggac gtaaccagta caatcgctcg caggtgacgg gcgcgggcgt cgacccgccg
     4501 ttgcacccgc acgcgcgcat ctcgggctcg gacgacgtcg acgcgcccta ccccgggctc
     4561 ctcgtgggcg gtggacagag cgcgagcaac tggcaagacg cgcagtccaa ttttcaaacg
     4621 aacgagatcg ccatcaactg gaacgcggcg ctggtctacg cgctcgcggg cttcatctcc
     4681 gacaagggcg cggccgcgtc gcccgcgcgc ggcgccctac ccgccggagc ctgtcaggtt
     4741 cgcgtgaact cggtcggcta cctccccgag cgcagcaagg tcgcgacggt cgtgagtgac
     4801 tgcacgaacg caggccctga tttccagtgc tcggtgaccg acaagacgct gagcggcgac
     4861 acgagcggcg cgctcagcat catcgacgac ctcgaagacg gcgatacgcg cgcgaccgca
     4921 aagcgaggcc gcagcggcgc atggttctcg tacgacgatg gtagcgcggg cacccgcacc
     4981 caagccgaga tcaagagcgc cggccgtgcc ggcagcaaga atgccatctg catcagcgga
     5041 cagggcttca ccggctgggg cggcggcatg ggcgtctcgc tggctggcga gggcagctcg
     5101 cgaatgctct acgacgcttc gccctacacg ggcgtgagct tctgggcgcg tggctcgggg
     5161 atgtttcgcg cgatgctcgt cgacaagtac agcgatccgg ccgcgtcgct gtgcacgagc
     5221 tgttacgacc atttccaagc cccgttcacg cctggctcgg aatggaagca gtacaccttc
     5281 agctggaaag atttgaagca gctcgggttc ggcgaaatga agcccaacgt gtgtgccgcc
     5341 gggctctacg cgctgcagtt tcagtggtcg gggagccagc cgttcgagct ctgcctcgat
     5401 gacgttgcgt tcacgcaagc agcgggcagc agcgactcgg ccgactctcc ttcggtgtcg
     5461 ccccgcggcg gaggctgcgg ctgccgcatc ggcgtcgtga gcgagtcgcc ggcttttgcg
     5521 gagctcggcg tactcgcgct cggactcacg gcggtgatga gtcgccgtcg tcgcgccgcg
     5581 cgggcgagcg cctcccgacg cggatcttga ggacgaaccg cacgtacgcg agcgcgacca
     5641 ggagactcaa accgaggccg agcgccgtcg cgaaggcgct cggcaagaaa cggacgtaga
     5701 gcgcgtgctg aactcgcgcc gcaccccaca acacgcccat cagcgacagc ggccccccga
     5761 ggcaaccgag gaattcccgc agtttgaccg gcatgatgcc gcgcaccatc aagaacagca
     5821 cgctgagggt gcacaggtac gtggccatcc acgcgaaaga gacggcgttg gcgccgagcg
     5881 tcgaaccgaa ggcgcgcacc aaaacccagc cgaacccgac gaagcacggc agcgtgaaga
     5941 gcgaatcgta ggcggcgagc tccggccgac ccgccgcgtg aaaaagctgc gggattgact
     6001 gcgtcagtgt ccgcaggaaa gcagcccagc acagcacttc gatcatgg
//
LOCUS       DASTKS010000379         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_729520, whole genome shotgun sequence.
ACCESSION   DASTKS010000379 DASTKS010000000
VERSION     DASTKS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729520"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2770
                     /locus_tag="VFQ35_07865"
     CDS             <1..2770
                     /locus_tag="VFQ35_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9930587.1"
                     /translation="NELKEQVKQVNGVSEVTLTGGERRQVRVLLDPERLAAHRLTASA
                     VAEALKAANQRLPAGTIAASNREFLLETGTVLANARDVGDVVVAASGGRVVFLRDVAS
                     IVDGPEEPSTYVRFGAGAAAEAGDRKLGAESVPAVTLSVAKHKGQNAVAIVDEVTRKI
                     DSLRGSLIPADVQLSVTRDYGATAAEKSNELLFHMGIAVVSVGFLIWLALGKRESGIV
                     MLAIPATLALTLTVFYLYGYTLNRITLFALIFSIGILVDDAIVIVENIARHFHLPENR
                     GRDLAEVAIRAVDEVGNPTILATMTVIAAILPMAFVGGLMGPYMRPIPVGASAAMLFS
                     LLVAFVVTPWATVRMLRHAHGASDEAEGRLTTFYRRVMGALLHVARLRYLFLAVVGVL
                     LVGAMALVAVQWVRVKMLPFDNKSEFQVVVDMPEGTPLEETDHVARALAEIVRKQPEV
                     INYQTYVGTSGPYNFNGLVRHYFLRQGPNVADIQVNLVDKGARKAQSHAIAKRIRDAM
                     APLAQRFGARIKVAEVPPGPPVLQTMVAEIYGPDYSRSVAIARDVRARLEKTNGVVDV
                     DWYVEDDQPKYRFVVDQEKAALNGVSEAEIVDALGLASAGRSTGLLHDEREKEDVPIV
                     LRLDRASRSDLARLQTIRVAGRDGSFVSIGELVRPELVTAEKSIYHKNLMPVVYVTAD
                     VAGAIESPVYAILKLGPELDAMKLPEGYALEQHTASQPFRNDTYAMKWDGEWHITYEV
                     FRDLGAAFAAVLVLIYVLNVAWFQSFKTPLVIMSAIPFSLVGILPAHGLLGAFFTATS
                     MIGFIAGAGIVVRNSIILVDFIEQRLAEGAPLDQAVIDAGAIRFRPMLLTAAAVIVGA
                     FVILFDPIFQGLAISLMAGEIASLALSRVTVPVVYYMMKRREERRHRTSAPTLTPSGG
                     AEALGAAE"
     gene            2910..3098
                     /locus_tag="VFQ35_07870"
     CDS             2910..3098
                     /locus_tag="VFQ35_07870"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9930588.1"
                     /translation="MSSNEGTVDRALRIVIGIVLLAAVFVGPKTPFGWLGVVPLVTGI
                     IGFCPLYRLIGIRTTKSA"
     gene            complement(3153..>3950)
                     /locus_tag="VFQ35_07875"
     CDS             complement(3153..>3950)
                     /locus_tag="VFQ35_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930589.1"
                     /translation="AFPNDNRGGFEPVMPTDPAACFGAQLNPLNQRFLTELAPADVLV
                     SLRANLAVLVNPSSDPDSVARASQALGAFVASLRAPNPGAVLQRLPIERAVRSLALPL
                     LACPSLPPYATLQSLGCFELKPTAECASLLDTVRGCSLWGATLEQVFPNGTLACRAAG
                     LDAKAEMESLMFAFDSNLKPVVGQQVTLGKNPSASVRARFELLVSAAQRGDADLVGFG
                     DAGGFAYENGSFRDSDGGRLSPSALLRQLTRCAPITFTAIPPSRYTN"
BASE COUNT          711 a         1312 c         1238 g          689 t
ORIGIN      
        1 caacgagctc aaagagcagg tgaagcaagt caacggcgtg agcgaagtca cgttgaccgg
       61 aggcgagcgg cgccaggtgc gcgtcctgct cgacccggag cgactcgcgg cgcaccgact
      121 caccgcgagc gcggtcgccg aggcgctcaa ggccgcgaac cagcggctgc cggcgggcac
      181 gatcgctgcg tccaaccggg agttcctgct ggagactggc accgtgctcg cgaacgcgcg
      241 cgatgtcggc gatgtcgttg tcgccgcctc cgggggacga gtcgtgttcc tgcgcgacgt
      301 ggcttcgatc gtggacggtc cagaggagcc ctcgacctac gtgcgattcg gagccggcgc
      361 cgccgccgaa gcaggcgatc gaaagctcgg cgccgagtcg gtaccggccg tgacgctctc
      421 ggtagccaag cacaaggggc agaacgccgt cgcaatcgtc gacgaagtca cccgcaaaat
      481 cgactcgctg cgaggttcgc tgatccccgc cgacgtgcag ttgtccgtca cgcgcgacta
      541 cggtgccacc gctgccgaga agagcaacga gcttctattt cacatgggaa tcgccgtagt
      601 cagcgtcggc tttctcatct ggctcgccct cggaaaacgc gagagtggca tcgtcatgct
      661 cgccatcccg gcgacgctcg cgctgacgct caccgtgttt tacctctacg gctacaccct
      721 caatcgcatc accctgttcg cgctcatttt ctcgatcggc atcttggtcg acgacgcgat
      781 cgtgattgtc gagaatatcg cgcgtcactt ccacctcccc gagaaccgag ggcgcgatct
      841 cgccgaggta gccattcgcg cggtcgacga ggtcggaaat ccgaccatcc tcgcgacgat
      901 gacagtcatc gcggccattt tacccatggc gttcgtgggc gggctgatgg gcccctacat
      961 gcgtccgatc ccggttggcg catcggcggc catgctgttc tcgctattgg tcgcgttcgt
     1021 ggtgacgccc tgggccaccg tgcgaatgct gaggcacgcc cacggcgcga gcgacgaagc
     1081 cgagggccga ctgactacat tttaccgccg ggtcatgggc gcgctgctcc acgtcgcgcg
     1141 cctgcgctac ctcttcctcg ccgtcgtcgg ggtactgctc gtcggagcga tggcgctcgt
     1201 cgcggtgcaa tgggtgcggg tgaaaatgct tcccttcgac aacaagagcg agttccaggt
     1261 ggtcgtcgac atgccggagg gcacgccact cgaagagacc gatcacgtcg cgcgagcgct
     1321 ggccgagatc gttcggaaac agcccgaggt aatcaactat caaacctacg tcggcacgtc
     1381 cggtccgtac aacttcaacg gcctcgtgcg gcactacttc ctgcgacaag gcccgaacgt
     1441 cgccgatatc caggtgaacc tcgtcgacaa aggcgctcgc aaggcgcaaa gccacgcgat
     1501 agccaagcgc attcgcgacg ccatggcacc gctcgcccaa cgcttcggtg cgcgcatcaa
     1561 ggtcgccgaa gttccacccg gccctcccgt gctccagacc atggtcgccg aaatctacgg
     1621 acccgactac tcgcgttccg tcgcaatcgc ccgtgacgtg cgggcgcgcc tcgaaaaaac
     1681 caacggcgtc gtcgacgtgg attggtacgt cgaggacgat cagccgaagt atcgcttcgt
     1741 cgtcgatcaa gagaaggcgg ccttgaacgg cgtgagcgaa gccgaaatcg tggatgcgct
     1801 cgggctcgcc agcgccggtc gttctaccgg tctcctgcac gacgagcgcg agaaggaaga
     1861 cgtgccgatc gtgcttcgcc tcgatcgcgc ctcgcgctcg gatctcgcgc gattgcagac
     1921 catccgcgtc gccgggcgcg acgggagctt cgtcagcatc ggcgaactcg tgcggccgga
     1981 gttggtcacc gccgaaaaga gcatctacca caagaacctg atgccggtcg tatacgtcac
     2041 cgccgacgta gcgggcgcga tcgagagccc agtgtacgca atcctcaagt tggggcccga
     2101 gctcgacgcg atgaagctcc ccgaaggcta cgccctcgag cagcacaccg cgtcgcagcc
     2161 tttccgcaac gacacgtatg cgatgaagtg ggatggcgaa tggcacatca cgtacgaggt
     2221 atttcgtgac ctcggcgctg cgttcgcggc ggtgctcgtc ttgatttacg tgctgaacgt
     2281 cgcctggttc caatccttca agaccccgct cgtcatcatg agcgcgatcc cattctcgct
     2341 cgtcgggatt ttgccggcgc acgggttgct cggcgccttc ttcacggcga cctcgatgat
     2401 cggtttcatc gccggcgccg gcatcgtcgt gcgaaactcg atcattttgg tcgacttcat
     2461 cgaacagcgc ttggcggaag gcgcaccgct cgaccaggcc gtcatcgacg cgggagcgat
     2521 ccgtttcaga cccatgctgc tcacggccgc ggcggtcatc gtcggcgcgt tcgtcatcct
     2581 cttcgatcca atctttcagg gcttggcgat ctccctcatg gccggcgaaa tcgcgtcgct
     2641 cgccctctcc cgcgtaacgg ttccggtcgt gtactacatg atgaagcgtc gcgaagagcg
     2701 ccggcaccga accagcgctc cgacgctgac gccctccggc ggagccgagg ctctcggcgc
     2761 ggcggagtaa ggtgcgactt tgctacatct cctcagaacg gagcccctgc tcagcgccgc
     2821 cttgcccccg acgagtctcg aacaacgagc gaagccgccg ccgaacccat ccgaacggcg
     2881 gctggctcct accctcgaag gagatcggca tgtcgagcaa tgaaggcacc gtcgaccgcg
     2941 ctctacgcat cgtgattgga atcgtgctcc tcgcagcagt gttcgtcgga ccgaagaccc
     3001 cgttcggctg gcttggggtg gttccactcg ttaccggcat catcgggttt tgccccctgt
     3061 accggctcat cggaatacgc acgaccaaat ctgcgtgagg ttcgtcgggg ccgccgcgca
     3121 accgcgcgta cgctccgccg taagtgaacc gctcagttcg tgtatcgact gggcggaatg
     3181 gccgtgaacg taatcggcgc gcaccgggtc aattgtcgca ggagcgccga gggtgacagc
     3241 cgcccgccat cgctgtctcg gaagctaccg ttctcgtagg cgaagccacc tgcgtcgccg
     3301 aagccgacga ggtcggcatc accgcgttgg gcggcgctca ccagcagctc gaagcgagcg
     3361 cgaaccgaag ccgacggatt cttgcccagt gtcacttgtt gaccgacgac gggcttcaag
     3421 ttgctgtcga aggcgaacat cagactttcc atctcggctt tggcgtcgag ccctgccgct
     3481 cgacacgcca acgtcccgtt tggaaacacc tgctcgagcg tagcgcccca gagcgaacat
     3541 cctcgaaccg tgtcgagtag tgaagcgcat tcagcggtgg gcttcagctc gaagcagcca
     3601 agcgactgca aggtcgcgta cggcggcaga ctggggcacg ccaacagcgg gagcgcgagg
     3661 gaccgaacgg cgcgctcgat gggcaagcgc tgcaatacgg cgcccgggtt cggcgctcgc
     3721 agcgaagcga cgaaggctcc caaagcctga ctcgcgcgcg ccaccgagtc cgggtccgag
     3781 ctcgggttca ccagcacggc aaggttcgcc cgcaggctca cgagcacgtc cgccggagcg
     3841 agctccgtca agaagcgctg gttcaacggg tttagctggg ctccgaagca cgccgccgga
     3901 tcggtaggca tgacgggttc gaacccgccg cggttgtcgt tcgggaaggc
//
LOCUS       DASTKS010000380         6633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1400302, whole genome shotgun sequence.
ACCESSION   DASTKS010000380 DASTKS010000000
VERSION     DASTKS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400302"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFQ35_07880"
     CDS             <1..514
                     /locus_tag="VFQ35_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930590.1"
                     /translation="LRPGARAAVQHLLDIGVEPVLLSGDARETCEALGHALDIDHIRP
                     ELQPSERGDAIRRLMDGGAIVAVLGRTPADDIALAAADVSIALSTAGSTASEWNVQLA
                     SDDVRDAAFALRLSHAARREARLALLATLTPAAALSVVAVLGLVTPATAPLAAAAGAA
                     LALLRLRALS"
     gene            953..1483
                     /locus_tag="VFQ35_07885"
     CDS             953..1483
                     /locus_tag="VFQ35_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9930591.1"
                     /translation="MPLDAEDSSEKMWGVKGTSPVGRSSHNPDLPRLRRDLDAYMAKR
                     RLRSTEQRRLIIDTLFDSGEHVTLDGLLRQVRAVDSRVGYATVYRTMKLLADSGIVQE
                     HKFGDGFTRYELNDEEHHHDHLICLDCGKITEFEEPLIEELQARVAQRYDFQVRQHKH
                     ELYGICSECQKPKKRR"
     gene            complement(1511..2140)
                     /locus_tag="VFQ35_07890"
     CDS             complement(1511..2140)
                     /locus_tag="VFQ35_07890"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET9930592.1"
                     /translation="MIHDPDMLAFLRDRARKQIRSRMRALRKAHPAAALAERSERIAE
                     RVRATDAYERARSVALFWPLAGEVDLRAVDAAARAAGKSVYYPVMDPIEGGVSTGFAL
                     SEQVSELAIRGSRFMEPPREARRALRSDIDLVIVPALAVGADGNRLGYGAGFYDVTLP
                     DFVPPALSMIVAYDFQLLAEIPTLPHDFACQLVVTDARTLVVGAEERKT"
     gene            complement(2214..3359)
                     /locus_tag="VFQ35_07895"
     CDS             complement(2214..3359)
                     /locus_tag="VFQ35_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930593.1"
                     /translation="MKKIVSVLGVLALFGACNSNDDAQDDTVTGGNAGASTKGGSGGQ
                     STAGKGGGGASSFGGKQNFPGGAGSGSGGEVSQAGMGPIETGGAGGSIADTAGAGGLG
                     GAGGTSDLPPEPVPSTDTCADAEATPNNDRTSATPFRLGTSYTACLQGPDDVDVYSFE
                     IPQDSRGGYVTVSINEVGPDGDISVSAYTAADNGRFYKTGSNTEGAAVYAYFTAKPGA
                     KFYLPVTHYLDNPKSNPYTLTVLYHQVPDVYEPNDLRNQAKQIAVGTPVEAYLFAGRE
                     YSTAIPDEDWLDWYKVDLVPGTLNVLLSPGANDIDGNIVLYDPDGVQIATKGSNTEGS
                     AVKLTEEITTAGTYYLKISPYEAPQAEGDTATVRQYFRLPYTLTVTQ"
     gene            3564..4295
                     /locus_tag="VFQ35_07900"
     CDS             3564..4295
                     /locus_tag="VFQ35_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9930594.1"
                     /translation="MRWPLKIAFVVPAYHAERTVAPVVVGLLALAATLPDTTAPAVVV
                     VDDGSTDETGRRAADAGAAVVRHAENRGKGAALLTGFAWAREAGARACVSVDADGQHP
                     PEEALALARHPAPPGALVLGVRDLLGAGAPRKNRFSNGFSNAWMSFFAKQSLHDTQCG
                     LRRYPLPEVLELGLRGTGYELESEVILKAIRRGIPVVETNVRVIYPPEHERVTHFHSV
                     RDPARIVARILHTAFTTRARRRAAP"
     gene            4292..5704
                     /locus_tag="VFQ35_07905"
     CDS             4292..5704
                     /locus_tag="VFQ35_07905"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HET9930595.1"
                     /translation="MTRRFWFRLAGVATLLVLLLMCGHFAVRRVGRMPAPNIELTRSQ
                     PTRDASGVRRLGKAFALDQHGVLEVHLEGSPREIGWAHARLLYPEMVENEGVLLTNFE
                     KAVPSWPLRLLLLDLAQLRYRDLSRGLTPERRNEIAAGALGFQPDPYSGVFPTYQRFV
                     YLNALYDIALSFEHSPLIGCTSFVFSGARAEHGGSLFGRNFDFEVDPIFDRKKAVFFV
                     RETGKVPFASVAWPGLVGVVSGMNLEGVALVVHGGRAGTPLAEGDPVVHTMRAVLSHA
                     RTSEEALSVLAQHPPLVSHIVVLTDAYGRGFRVERVPGARDFAAPLGDAAAVTNHLEG
                     PSRLDPKNQSVREHTSTVPRKARADELVALAPPVVDSAFVVGALRDRKAYGGRPLAPG
                     DRNAIDAHIATHAVVMDTAKRVLWVSTGPHLAGRFVAFDLTRRLGEGYLPEPNADLPQ
                     IPADPSLLAPNDALFDKRAQ"
     gene            5720..6487
                     /locus_tag="VFQ35_07910"
     CDS             5720..6487
                     /locus_tag="VFQ35_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930596.1"
                     /translation="MSIQSSRWLLAAPLLLLPCRALAQDNSTLSSGGLAPPPPVESTP
                     EPTAPTTTEQELTRADKEDSGRGLEFVWLNAEAGFQHLGLETFHANKLVDANVVQSTQ
                     TGLLVGAGAGVRLIFLTGGVRFRLGNFSAWQLWTLDAELGLRIPLGALEPYFTFAGGY
                     ARMGSFSTSNVFDTNNLNVRGFNLRAGFGLDYYFAKSFSLGANASGDILFLSRPKVSQ
                     QPAGNDPQSQAAAQVYAQDGTSVGAGVTVTAVAGLHF"
BASE COUNT         1061 a         2276 c         2138 g         1158 t
ORIGIN      
        1 cctccgcccc ggcgcgcgag ccgccgtcca acacttgctc gacatcggcg tcgaaccagt
       61 gctgctctcg ggagacgcgc gcgagacgtg cgaagcgctc ggtcatgcgc tcgacatcga
      121 tcatattcgg ccagagcttc agccttcgga gcgaggtgac gcgattcgaa ggctcatgga
      181 cggcggcgcg atcgtcgcgg tcctcgggcg cacgccggcc gatgacatcg cgctcgcggc
      241 tgcagacgtg tccattgcgc tctcgaccgc gggcagcacc gcttcggagt ggaacgtaca
      301 actcgcctcc gacgacgtgc gcgacgccgc ctttgctctg cggctatcgc acgcggcgcg
      361 acgggaagct cgcctcgcgc tgctcgcaac gctcacgccc gccgctgcgc tcagcgtcgt
      421 cgcggtgctg ggcctcgtca cgcccgccac cgcaccgctc gctgccgctg ccggcgccgc
      481 gctcgccttg ttgcgtctgc gcgcgctgag ctgagggtcg tgctcgcgct tccggtccgc
      541 ccatacctcg agcaatgcag cgcgcggccg ccacatcctc gcgcactacg acgacgatag
      601 catcgtcgtc tatgaagcgt accgagcgga gatagccgac ctcgcgatcg agcacgaacg
      661 ctttggccgc cccttcagct tctcgcgcat gaccacgctg actgggcctc tcttcagacg
      721 ccgagcaagc gcgtgttcct gccgcgatcc agcgaggcgg ctcgaaacgt aacgctcgac
      781 gcggaggtca ccaccaaccc tccccccaag gcgtgaggct ggcgtgtgca ccggtgcggc
      841 gggcgagcta gcccgacgct cggttgaaat cccgcccaag gcagccgggg cgaaaaggca
      901 acgccgcgcg ccacgagcct ggctcccttt caaaacggcc ggagaaggga atgtgccgct
      961 tgacgcggaa gacagttcgg aaaagatgtg gggagtgaag ggcacctcac ccgttggacg
     1021 aagctcgcac aacccggatc ttccgcggct gcgacgggac ctcgacgctt acatggcgaa
     1081 gcggcggttg cgctccacgg agcagcgccg gctcatcatc gataccttgt tcgactccgg
     1141 tgagcacgtg accctcgatg gcctcttgcg ccaagttcgc gcggtcgatt cgcgcgtcgg
     1201 gtacgccacc gtataccgga cgatgaagct cctcgcggat agcggcatcg ttcaggagca
     1261 caagttcggc gacggcttca cgcgctacga gctgaacgac gaggagcacc atcacgatca
     1321 cctgatttgc ctcgactgcg gcaagatcac cgagttcgag gagccgttga tcgaggagct
     1381 ccaggcccgc gtggctcagc gttacgactt tcaggtacgc cagcacaagc acgagctcta
     1441 cggcatctgt tccgagtgtc agaagccgaa gaagcgacgc tgagctccgc gtcgcaccga
     1501 aggcactacc ctaggtcttg cgctcctccg cgccaacgac gagcgtgcgt gcgtccgtca
     1561 cgaccagctg acatgcgaag tcgtgcggta gcgtcgggat ctcggcgagg agctggaagt
     1621 cgtacgcgac gatcatactg agtgcgggcg gcacgaagtc gggcagcgtc acgtcgtaga
     1681 agccagctcc gtagcccaag cgattgccgt cggcgccaac cgccaacgcg ggcacgatca
     1741 cgaggtcgat gtccgagcgg agggcgcgcc gagcctcgcg cggaggctcc atgaagcggc
     1801 tcccccggat cgcgagctcg ctcacctgct cgctcagcgc aaacccggtc gaaacgccgc
     1861 cctcgattgg gtccatcacc gggtagtaga ccgactttcc cgccgcccgc gctgccgcat
     1921 cgacggcgcg gaggtcgacc tcacccgcga gcggccagaa gagcgctacc gagcgcgctc
     1981 tctcgtatgc gtccgtcgcc cgcacgcgct cggctatccg ctcgctgcgc tctgccagcg
     2041 ccgcagccgg gtgagccttc cgcagagcgc gcatccgcga acgaatctgc tttcgcgctc
     2101 gatcgcgcag gaacgcgagc atatccgggt cgtgaatcac tcggccagcg tagcgcgttt
     2161 cgctcggcgc gccgcgcgag gacgcatagt gcccccgcgc ggctcgaacg cccttactgc
     2221 gtcaccgtga gcgtgtacgg gaggcggaag tactggcgca cggtggccgt gtcgccctcg
     2281 gcttgcggcg cctcgtacgg cgaaatcttc aagtagtagg tgcccgccgt cgtgatctcc
     2341 tcggtgagct tcacggccga gccttcggtg ttgctgccct tggtcgcgat ctgaacgcca
     2401 tcgggatcgt agagcacgat attgccgtcg atgtcgttgg cgccgggcga gagcaatacg
     2461 ttgagcgtgc ctggcaccaa gtcgaccttg taccaatcga gccagtcttc gtcggggatc
     2521 gccgtcgagt actcgcggcc tgcgaacagg taagcctcga cgggagtgcc tacggcaatt
     2581 tgctttgcct gattgcggag gtcgttcggc tcgtaaacgt ccgggacctg gtggtagaga
     2641 acggtgagcg tgtaggggtt cgacttcggg ttgtcgaggt agtgggtgac cgggaggtag
     2701 aacttcgcgc caggcttggc cgtgaaatag gcgtagaccg cagcgccctc ggtgttgctc
     2761 ccggttttgt agaacctgcc gttgtcggcc gcagtgtacg cgctgacgga gatatcgccg
     2821 tcgggtccaa cctcgttgat gctcacggtg acgtatcccc cgcgtgagtc ttgcgggatc
     2881 tcgaaagagt agacgtcgac atcgtccggg ccctgcaaac aagccgtgta gctcgtgccg
     2941 agccggaagg gtgtcgcgct cgtgcggtcg ttgttcgggg tcgcctccgc gtccgcacac
     3001 gtatccgtgc tgggaaccgg ctcgggcggg agatcgctcg tgccgccagc gccgccgagg
     3061 ccaccagcgc cggcagtatc cgcgattgaa cccccagcac cgccggtctc gatcgggccc
     3121 atgccagctt gcgacacctc gcctcccgag ccgctccccg cgccgccggg gaagttctgc
     3181 ttgccgccaa agctcgatgc gccgccgccg cccttgcctg ccgtcgactg cccgccgctg
     3241 ccgcctttgg tggatgcccc ggcatttcca ccggtcacgg tgtcgtcctg ggcgtcgtcg
     3301 ttcgagttgc aggcgccgaa caaggcgaga acacccaaga ccgatacgat tttcttcatg
     3361 acgaactatc tcccctaatc tcgcgcgcgg agcggtcgct cgaaccgcgc ccactcgacg
     3421 ccccgacaca taggatacgg tgagcgcgct agcaagctcg gtcgctggtt ctataagggc
     3481 gccggaagca ccgcgagccg agtcacggcc tccaaaatcg cctcataaag cgcgaaatgc
     3541 gcgacgctct ggcctgtgcg acagtgcggt ggcccttgaa gatcgccttc gtcgttcccg
     3601 cttatcatgc cgagcgcacc gtagcgccag tcgtcgtcgg attgctcgcg ctcgcggcaa
     3661 cgttaccaga caccaccgcc ccggccgtcg tcgtcgtcga cgatggttcg accgacgaaa
     3721 ctgggcggcg cgccgcggac gccggcgcgg cggtcgtacg ccacgcagaa aatcgaggga
     3781 agggcgcggc cctgctcact ggcttcgcct gggcgcgtga agccggcgcg cgcgcttgtg
     3841 tcagcgtcga cgccgatggg cagcatccgc ccgaagaggc gctcgcactg gcgcggcatc
     3901 ccgcgccgcc cggcgcgctg gtgctcggcg tgcgcgatct cttgggagcg ggggcgcccc
     3961 gaaagaatcg cttttcgaac ggattttcga atgcgtggat gtcgttcttc gccaagcagt
     4021 cgttgcacga cacgcagtgc ggcctgcggc ggtatccgtt gccggaggtg ctcgagctcg
     4081 gcttgcgcgg cactggttac gagctcgagt ccgaagtgat cttgaaggcg atccgacgag
     4141 gtattccggt cgtcgagacc aacgtccgcg tcatctatcc gcccgagcac gagcgcgtta
     4201 cgcactttca cagcgtgcgc gatccggcgc gcatcgtcgc gcgcatcctc cacaccgcgt
     4261 tcacgacgcg cgcgcggcgc agagcagcgc catgacgcgt cggttctggt tccgcctcgc
     4321 gggggtcgcg acgctgctcg tgctgctcct catgtgcggc cacttcgcgg tccggcgcgt
     4381 ggggcgaatg ccggcgccga acatcgagct cacccgttcg cagccgaccc gcgacgcgag
     4441 cggagttcga cggctgggga aggcgttcgc tctcgaccaa cacggggtgc tcgaggtgca
     4501 cctggaaggc tctccgcgag aaattggttg ggcccacgcg cgcttgctct acccagagat
     4561 ggtcgagaac gagggcgtgc tgctcaccaa cttcgaaaaa gcggtgccga gctggcctct
     4621 ccgcctcctg ctgctcgatc tcgcccagct caggtaccga gatctctccc gcgggctcac
     4681 ccccgagcgc cgcaacgaaa tcgccgccgg cgcgctcggc ttccaacccg acccctactc
     4741 cggcgttttt ccgacgtacc agcgcttcgt gtacctgaac gcgctgtacg acatcgcgct
     4801 ctcgttcgaa cactcaccgc tcatcggctg cacgagcttc gtgttctccg gcgcgcgcgc
     4861 cgagcacggc gggtcgctct tcggtcgcaa tttcgatttc gaagtcgatc ccatcttcga
     4921 tcgcaagaaa gccgtcttct tcgtgcgcga aaccggcaag gtgccgttcg cgtcggtggc
     4981 gtggccgggc ttggtcgggg tggtcagcgg catgaacctc gagggggtgg cgctcgtcgt
     5041 gcacggaggt cgagccggaa cgccgctcgc agagggcgac ccggtggtgc acacgatgcg
     5101 cgcggtgctg tcgcacgccc gcacgagcga ggaggcgctc tccgtgctcg cgcagcatcc
     5161 gccgctcgtg agccacatcg tcgtcttgac cgacgcctac ggacgcggct ttcgggtcga
     5221 gcgcgtaccc ggtgcgcgcg atttcgcagc cccgctcggc gatgctgcgg ccgtgaccaa
     5281 tcacctcgag gggccgtctc gcctcgaccc gaagaaccag tcggtgcgcg agcacacctc
     5341 gaccgtgccg cgcaaggcac gcgctgacga gctcgtcgct ctggccccgc cggtcgtgga
     5401 ctccgcgttc gtcgtcggag ccctgcggga tcgcaaagcg tacgggggaa ggccgctcgc
     5461 gccgggcgat cgcaacgcca tcgacgcgca catcgctact cacgccgtcg tgatggatac
     5521 ggccaaacga gttctgtggg tgagcacggg accacacttg gccggtcgct tcgtggcctt
     5581 cgacctgacg cgaaggcttg gcgagggtta ccttcccgag ccgaacgcag acctgcccca
     5641 gatcccggcc gatccgagcc tcctcgctcc gaacgacgct ctgttcgaca agcgcgcaca
     5701 gtgattttag actgacgcaa tgtcgattca gagctcgcgt tggctgctcg ccgccccgtt
     5761 gttgctctta ccctgccgag cgctcgcaca agacaactcc acgttgagct ctggggggct
     5821 agcgccgccg ccgcccgtcg agtccactcc cgaacccacc gcgcccacga ccaccgagca
     5881 agagctcacg cgcgccgaca aagaggactc gggccgcggc ctcgagttcg tgtggttgaa
     5941 cgccgaggcc gggtttcaac acctcggcct cgaaaccttt cacgcgaaca agttggtcga
     6001 cgcgaacgtc gtgcagtcga cgcagactgg gctcttggtg ggcgccggcg ctggtgtacg
     6061 gctcatcttt ctcaccggcg gcgtgcgctt tcggctcggc aatttcagtg cgtggcagct
     6121 ctggactctc gacgccgagc tcgggctcag aattcctctc ggtgctttgg aaccgtactt
     6181 caccttcgcc ggcggctacg cgcgcatggg ctcgttcagc accagcaacg tgttcgacac
     6241 gaacaacctg aacgtccgcg gcttcaacct gagggcaggg ttcggcctcg actactactt
     6301 tgcgaagtcg ttttcgctcg gcgcaaacgc ctcgggcgat atcctctttt tgtctcgccc
     6361 gaaggtcagc caacagccgg ccggcaacga ccctcaatct caagccgcgg ctcaggtgta
     6421 cgctcaagac ggcaccagcg ttggcgctgg cgtgaccgtg accgcggtcg ccggcctgca
     6481 tttttgaggg agcccgaacc aggtgaagct cctcttcgca ctgctcgtcg cggcgttcgc
     6541 gctcggcctc ggtgcgctcg tcttccataa ggtccgcgcg acgggcggca tttctcgtcg
     6601 gacggcgctc tacgcagcga tcgggggggc cat
//
LOCUS       DASTKS010000381         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_61893,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000381 DASTKS010000000
VERSION     DASTKS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61893"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1567)
                     /locus_tag="VFQ35_07915"
     CDS             complement(5..1567)
                     /locus_tag="VFQ35_07915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9930597.1"
                     /translation="MKTAELASAFDVSFQLRERDDGGLLQLSRCRELLHEIVHSDSVR
                     RSELDALYRELGELANELGAARRVSGFRVNDEAVPSEPKGPNESATWRTLEPSLLPPS
                     ALDLPTDTRTALLALLDESPVPLLLARLGDGRICYANRCAGELFQIGTAALIERPLDD
                     FLDDVRANASFWAELASKASSEGTLLAFIEPRSDKRFWTLTSARLITHDGERCAFLGL
                     FDVTAQKVAERELRRNESNLRSLLGAAPIALLGLSPGTKEILLANERANALLGARDRP
                     LIGQSFLNFVWDPKESAAFVRAVAERGPIEGLSLKLRSLAGKQRWVMLSAEWLEVDSD
                     PIVLVGITDVTEQKELEQRLVELATHDGLTGLYNRRHFVDLAERELSRAARSRCPVSL
                     CMLDADQFKQMNDEFGHAVGDRALTTLARATRGMLRAQDIFGRLGGEEFGVLLPDTRI
                     EGALAVAERVRRSVQMTQVPTAHSAPVWITVSIGVAQHRPGESFETLMRRADHALYRA
                     KDRGRNRVERED"
     gene            complement(1681..2259)
                     /locus_tag="VFQ35_07920"
     CDS             complement(1681..2259)
                     /locus_tag="VFQ35_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930598.1"
                     /translation="MADSILIVMALEVEAQGRFADEPVLFTGIGKVNAAYRLTRRLAE
                     ERAAGRKPLVVNFGTAGSRSYPTGSVIACRRFVQRDMDVRELGFALGVTPFEDTPREL
                     EFEPVFPALPEGVCATADRFETLDHAPEWEVIDMEAYALAKVCALEGVTFACVKFITD
                     GADSNASTDWQTNLPRAAEAFVALYESLKKQG"
     gene            complement(2252..2758)
                     /locus_tag="VFQ35_07925"
     CDS             complement(2252..2758)
                     /locus_tag="VFQ35_07925"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9930599.1"
                     /translation="MSDLKRYINEVPDFPKPGILFRDIAPLLREQFVPTIAALDTLVG
                     EARWRDIDAIAGIDSRGFILGAGLAARKNKGFVPVRKQGKLPPPVVDVTYDLEYGTGM
                     LEMQPGKGRVLLVDDVLATGGTLRAAADLCSQAGYSVTGLLVLIDLRLGQDFVWRSLP
                     AQALIHYG"
     gene            3000..4604
                     /locus_tag="VFQ35_07930"
     CDS             3000..4604
                     /locus_tag="VFQ35_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930600.1"
                     /translation="MLKRLSVRGFALLADVAVEFGPGLNVFTGETGAGKSLLLGALAA
                     MVGRGLPTGLAGPGCDAHIDLEIANDATIRAALRVRDDKVRCFLDQKSVPVGELRRKV
                     GDSLILTAQGAVRDLASPAGVLELLDQRAGTLPLLAEYRAARREATRVGREIVELQAE
                     LGAKRAREGRVGEWIDELEKLAPRPGEYAALGARIELLSRRTQYLELCGRVMSVLSER
                     DVSVESELRALLTALRRSAAPTFGEIAESLSEALACVEAASSRAAAAAEELASESLGL
                     EELEQRRDAFERMAKRLACEPDALDGAAAELERERARLEAIEQQLVELEQKRTVAEAQ
                     ARELGERLHSRRQAAVPPLLKELGRELAWLSLEGASLDVQLERRAEALGPDGPSTLRL
                     GFSANPGQAPGPLERVASGGERSRFAMALACAGAAPGRTLIFDEIDQGVGGEALARLA
                     ERLSLLARTRQVVCVTHQAAIAARAERHFRVEKSVERGRTTTRLALLDEEARTSELSR
                     MLAGGRASEGARVLARRLRDEARSAA"
     gene            complement(4614..>4954)
                     /locus_tag="VFQ35_07935"
     CDS             complement(4614..>4954)
                     /locus_tag="VFQ35_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HET9930601.1"
                     /translation="VYYAGPAKTPEGYASGSFVMLAKGNRSPAVTAACKKHGGFYLGS
                     IGGPAARLAKDCIRHVEVLEYPELGMEAVWKIDVVDFPAFIVVDDKGNDFFAEIVGGR
                     SAASLPIAKS"
BASE COUNT          814 a         1611 c         1683 g          846 t
ORIGIN      
        1 ccccctagtc ttctcgttcg actcggttcc gcccgcggtc cttcgctcgg tagagggcgt
       61 gatctgcgcg tcgcatcagc gtctcgaagc tttctcccgg acggtgctgg gcgaccccga
      121 tgctcacggt gatccagacg ggggcgctgt gggcggtcgg cacctgcgtc atctggacgg
      181 agcgccggac gcgctcggcg acggcgagcg cgccttcgat acgagtatcc ggcaggagca
      241 cgccgaactc ctcgcctccc aggcgcccga agatgtcttg cgcgcgcagc atgccgcgcg
      301 tcgcgcgcgc caaggtcgtg agcgcgcgat ctcccaccgc gtggccaaac tcgtcgttca
      361 tctgcttgaa ctggtccgcg tccaacatgc acaagctgac tggacagcgg ctgcgcgccg
      421 cgcgcgagag ctcccgctcg gccagatcga cgaagtgtct gcggttgtac aagccggtca
      481 agccgtcgtg agtcgcgagc tctacgagcc gttgttcgag ctccttctgc tccgtgacgt
      541 cggtgatccc aaccagcacg atcggatcgc tgtcgacctc cagccattcg gcggagagca
      601 tcacccagcg ctgcttgccg gcgagcgagc gcagcttcaa cgacaagccc tcgatgggcc
      661 ctctctctgc gaccgctcgg acgaacgcgg cggactcttt tggatcccac acgaagttca
      721 agaacgattg tccaatcaat gggcggtcgc gcgcgccgag cagagcgttc gcgcgctcgt
      781 tcgccagcaa gatctccttg gttcccgggc tcaacccgag cagcgcgatg ggggccgcgc
      841 cgagcagtga gcgcaggttc gattcgttcc gacgcagctc gcgttcggcg accttctgcg
      901 cggtcacgtc gaagaggccc aagaaggcgc atcgctcacc atcatgagta atcaggcgcg
      961 cgctcgtcag ggtccagaag cgcttgtcgc tgcggggctc gatgaaggcg aggagcgtcc
     1021 cttcgctcga cgccttgctc gcgagttccg cccagaagct cgcgttcgcg cgtacgtcgt
     1081 cgaggaagtc gtcgaggggg cgttcgatga gcgccgcggt tccgatctgg aacagctcgc
     1141 cggcgcagcg gttcgcgtag caaatgcgcc cgtcaccgag tcgcgcgagg agcagcggta
     1201 cgggcgactc gtcgagcagc gcgagcagcg cggtgcgcgt gtcggtcggc aaatcgagcg
     1261 cgctcggcgg gagcaggctg ggctccagcg ttcgccacgt tgccgattcg ttgggcccct
     1321 ttggttcgga aggaaccgct tcgtcgttca ctcggaagcc actcacgcgg cgagccgcgc
     1381 cgagctcgtt cgcaagctcg ccgagctcgc gatacagtgc gtcgagctca cttcttcgga
     1441 cgctgtccga gtgcacgatt tcgtgcaaaa gctccctgca acgtgagagc tgcagcagcc
     1501 ctccgtcgtc cctctctctc aattgaaagc tcacgtcgaa cgccgacgcg agctcggccg
     1561 tcttcattca gcacagcata cgctctcggc ttcgacgtaa aaagtggcgg attgcgtggc
     1621 ggattacggc actgtcggtt gccagggtgc gtcccgaatc gggatgtgtg gccagcgtgt
     1681 tcaaccctgt ttttttagtg attcgtagag ggcgacgaac gcttccgccg cacgaggcaa
     1741 attggtttgc caatcggtgc tcgcgttcga atcggccccg tcagtgatga atttcacaca
     1801 cgcgaacgtt acgccctcta gcgcacacac tttcgcgaga gcgtacgcct ccatgtcgat
     1861 gacctcccat tcgggcgcgt gatcgagcgt ttcgaagcgg tccgcggtcg cgcagactcc
     1921 ctcgggcaac gccgggaaaa ccggctcgaa ctcgagctct cgaggggtgt cttcgaacgg
     1981 cgtcacgccc agagcgaacc cgagctcgcg cacgtccatg tcgcgctgca cgaagcgccg
     2041 gcaggcgatg acgctaccgg tgggatagct gcggctgcca gcggtgccga aattcacgac
     2101 gagcggcttt cgaccagcgg cgcgctcctc cgccagccgc cgcgtcagac ggtacgccgc
     2161 gttgaccttg ccgatgccag tgaagagcac gggctcgtcc gcgaagcgcc cctgggcttc
     2221 gacttcgagc gccatcacga tgaggatcga gtcagccata gtgaatcagc gcttgcgcgg
     2281 gcagcgaacg ccaaacgaag tcttgtccga gtcgcagatc gatgagcacg agcaaaccgg
     2341 taacgctgta acccgcttgc gaacacaggt ctgccgctgc gcgcaaggtc ccgccggttg
     2401 cgagcacgtc gtcgaccaac aacacgcgcc ctttgccggg ctgcatttcg agcatgccag
     2461 tgccgtactc gaggtcgtag gtgacgtcga cgacgggggg aggcagcttg ccttgcttgc
     2521 ggaccggcac gaatcctttg ttctttcgag ccgcgaggcc cgcccccaga atgaagccac
     2581 gcgaatcgat cccggcgatc gcgtcgatgt cacgccagcg cgcctctccg accagcgtgt
     2641 cgagcgcggc gatggtcggt acgaactgct cgcgaaggag cggcgcgatg tcgcgaaaga
     2701 ggatcccggg tttgggaaag tcggggactt cgttgatgta acgtttgagg tcgctcatgg
     2761 cactcgcagg ggtggagatt cgctccatga ggtccgcgcg actaggggga tgtcccaagt
     2821 tctcccccgg ttgcgagcga ggaatcgccc gtctgggagc tcgaccgacc tcgggcgcgc
     2881 cgcggcgatg gtccgagctc gacgttacgc cgtaaaaact gccgggagga ctcgatccgg
     2941 acagacaatg tccgagctgg cgctagtcgg agtcgcgcgt ttttggcatg catgcctaca
     3001 tgctgaagcg actttcggtg cgcgggtttg cgttgcttgc ggacgtggcc gtggagttcg
     3061 ggccgggtct caacgtgttt acgggcgaga ccggcgcggg aaagtctttg ctgctcggtg
     3121 cgctcgccgc gatggtcggg cggggtctgc ccacgggcct tgcggggccc ggctgcgacg
     3181 cgcacatcga tctagaaatc gcgaacgacg cgacgatccg cgcggcgctt cgcgttcgcg
     3241 acgataaggt caggtgcttc ttggatcaaa agagcgtgcc ggtgggggag ctccgtcgca
     3301 aggtcggtga ttcgttgatc ctcaccgcgc agggcgcggt gcgcgacctc gcgagcccag
     3361 ccggggtcct cgagctcctg gatcagcgcg ccgggacgtt accgctgctc gccgagtacc
     3421 gcgccgcccg tcgcgaggcg acgcgggtag gtcgcgaaat cgtcgagctg caagcggagc
     3481 tcggcgccaa gcgtgcacgc gaaggaagag tcggcgaatg gatcgacgag ctcgaaaagc
     3541 tcgcgccgcg cccgggcgag tatgcggcgc tcggcgcccg catcgaactc ctctcgcgcc
     3601 gcacccagta tctcgagctc tgcgggcgcg tgatgtcggt gctctcggag cgtgatgttt
     3661 cggtcgagag cgagctgcgg gcactgctta ccgccctcag gcggagcgcg gctccgacct
     3721 tcggggagat cgccgagagc ctgagcgagg cgctcgcgtg cgtggaagcg gcctcgagcc
     3781 gggccgccgc ggcggctgaa gagctcgcga gcgagtcctt ggggctcgag gagctcgagc
     3841 aacggcgcga cgcgttcgag cgcatggcca agcgtctcgc gtgcgagccc gacgccctcg
     3901 acggggcggc ggccgaactc gagcgagagc gcgcccggct cgaggcaatc gagcagcagc
     3961 tagtagagct cgagcaaaag cgcaccgtag ccgaggctca ggctcgtgag ctcggcgagc
     4021 gccttcactc gcggcgacaa gccgcagtgc ctccgttgct caaagagctc gggcgcgagc
     4081 tcgcgtggct ctcgctcgaa ggcgcatccc tcgacgtgca gctcgagcga cgcgcggagg
     4141 cgctcggtcc cgacggcccg agcacgcttc gtctcggctt ttctgcgaac cctgggcaag
     4201 caccggggcc gctcgagcga gttgcgtccg gcggcgaacg ctcccgcttc gccatggcgc
     4261 tcgcgtgcgc cggggctgct ccggggcgca ccctgatttt cgacgagatc gatcaggggg
     4321 tcggagggga agcgttggcc cgcttggccg agcgcttgtc cttgctcgcc cgtactcggc
     4381 aagtggtgtg cgtcacccat caagccgcga tcgccgcccg cgccgagcgc cacttccgtg
     4441 tagagaagtc ggtcgaacgc gggcgtacga cgacgcggct cgcgctgctc gacgaagaag
     4501 ctcggacgag cgagctctcg cgcatgctcg cgggagggag ggcgagcgaa ggcgcgagag
     4561 tgctcgcgcg tcgcttgcgg gacgaggcgc gctcggcggc ctgacgaaac tcctcagctc
     4621 ttcgcgatgg gcaaactcgc ggcggagcga cctccgacga tttcggcgaa gaagtcgttg
     4681 cccttgtcgt cgaccacgat gaacgccggg aagtcgacta cgtcgatctt ccatacggcc
     4741 tccatgccga gctccgggta ctcgaggacc tcgacgtggc gaatgcagtc cttcgcgagg
     4801 cgcgccgcgg gaccaccgat cgagcccaaa tagaaaccgc cgtgcttctt gcacgcggcg
     4861 gttacggcgg gcgaccgatt gcccttggcc agcatcacga agctaccgga ggcgtagccc
     4921 tccggcgtct tcgcggggcc agcgtagtac acca
//
LOCUS       DASTKS010000382         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_508108, whole genome shotgun sequence.
ACCESSION   DASTKS010000382 DASTKS010000000
VERSION     DASTKS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508108"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VFQ35_07940"
     CDS             <1..1097
                     /locus_tag="VFQ35_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930602.1"
                     /translation="GASSGAGGASVGGGGSAGAGAGGNGGAGGYVPSGPCSPPVDIKK
                     PVEKLSQTGCMSSDMKRMADVVIAYDVNSPLWSDSADKTRGMVVPQGQKVHVLNCKGN
                     PDECRYGEQDDGHWVFPIGTVLVKNFQFDGKFVETRLLVRRDTKVWVGYSYQWNEEQT
                     DATIVGDPGASVMFNTGKRQVAWNYPSRYDCMLCHAKPGGYSIGPETRQLNKMLNGEN
                     VLDKLERLGTFDAPLPKPYLAPLPTPYDSFEGKPPAGATSEELARSYLHANCGFCHRA
                     DGNPGTLDLRWGVPLKDTASCNVRPQKGDAGLTTYTILTPGKPDESVLWARMKTLDDK
                     NRMPQIGTVVVDEPGLKVVGDWITSLKDCQ"
     gene            complement(1120..1614)
                     /locus_tag="VFQ35_07945"
     CDS             complement(1120..1614)
                     /locus_tag="VFQ35_07945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9930603.1"
                     /translation="MKVLAICGSLQRRSSNLTLLQAAQNAAPVGVEVNLFDGVARLPH
                     FDPDLEADEPLQVVEEWRVALRSSDAVLIATPEYGHSLPGALKNAIDWVIGSGELYQK
                     PVAITAAVPTSERGRRGLAALRQVLEAVDARIVNDEPIVRGELFERDVAALLNALIER
                     ARPR"
     gene            1952..>2244
                     /locus_tag="VFQ35_07950"
     CDS             1952..>2244
                     /locus_tag="VFQ35_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930604.1"
                     /translation="MMGNDEFLLTSGAEEYSISLRSSGAAPPPPPSEALVSRVVFELR
                     YGLEAGRSEVVLLARRLLAALDGSGFPRSLLDDASAAEAGELLSLVEREIRAGR"
BASE COUNT          409 a          685 c          753 g          397 t
ORIGIN      
        1 gcggtgctag ctcaggtgcg ggcggcgcat cggtcggtgg cggcggaagc gcaggtgcgg
       61 gtgcaggtgg aaacggcggt gccggtggct atgtaccgag cggcccgtgt agcccccccg
      121 tcgacatcaa gaagcccgtc gagaagctca gccagacggg ctgcatgtcg agcgacatga
      181 agcggatggc agacgtcgtg atcgcgtacg acgtgaacag cccgctttgg tcggattcgg
      241 cggacaagac gcgcggcatg gtcgtgcctc agggacagaa ggtccacgtc ttgaactgca
      301 aaggcaaccc ggacgagtgc cgttacggcg agcaagacga cggccattgg gtatttccaa
      361 tcggcacggt gctcgtgaag aactttcaat tcgatggcaa gttcgtcgag acgcgcttgc
      421 tggtacggcg cgataccaag gtttgggtcg gctacagcta ccaatggaac gaagagcaaa
      481 ccgatgccac gatcgtcggg gatccgggcg cctcggtgat gttcaatacc ggcaagcggc
      541 aagtggcgtg gaactacccg agccggtacg actgcatgct ttgtcatgcc aaacccggtg
      601 gctactccat cgggccggag acgcgacaac tcaataagat gctgaacggc gagaacgtgc
      661 tcgacaagct cgagcgcctc ggcacgttcg acgcaccgct gccgaagccc tatctcgcgc
      721 ccctcccgac cccgtacgac tcgttcgagg ggaagccgcc cgcgggcgca accagcgagg
      781 agctcgcgcg ctcgtacttg cacgcgaact gcgggttttg tcaccgcgcg gatggtaacc
      841 ccggcactct ggatctgcgc tggggtgtgc ccctcaaaga cactgcttcg tgcaatgttc
      901 ggccacaaaa gggcgacgcg ggcctcacca cgtacacgat tctcacgccc ggcaagccgg
      961 acgagtccgt gctctgggcg cgcatgaaga cgctcgacga caagaaccgc atgccgcaga
     1021 tcggcacggt cgtcgtcgac gagccggggc tcaaggtcgt gggcgattgg atcacttcgc
     1081 tcaaggactg tcagtaggcc ggttcggcgg aggcgcttct caacgcggcc gagctcgctc
     1141 gatcagcgcg ttgaggagcg ccgcgacgtc gcgttcgaaa agctcgccgc ggacgatcgg
     1201 ttcgtcgttc acgatacggg cgtcgaccgc ttccaagacc tgccgcaatg ccgcgagccc
     1261 gcgtcggcct cgctcggacg tcggtacggc agctgtaatc gcgactggct tttggtacag
     1321 ctcgcccgag ccgatcaccc agtcgatcgc gtttttcaag gctccgggaa ggctatggcc
     1381 gtactcgggt gtcgcgatca agaccgcgtc gctcgaccgc agcgctacgc gccactcttc
     1441 gacgacttga agcggttcgt ctgcctcgag atcgggatcg aagtgcggca accgcgcgac
     1501 tccgtcgaag aggttcacct cgactccgac cggtgccgcg ttttgcgcag cctgcagcag
     1561 agtgaggttg ctcgaccgcc gctgcaagct cccgcaaatg gctagaactt tcatgtgagt
     1621 tcgtagctcg ctcgcaggtg cgttagcaag cgcgcaacga cataccagcg actcggacat
     1681 cgctgctccc gaccgaggga cgagcagaaa cctttgaacg agcttgcaga tatctcgctg
     1741 cgacgagctc cggggcggga gctcggcgtc ttggcgaaag tggctgtgtt gctgcggtct
     1801 tggcgcgacg ttcgagtgga ttgatcgctc gtttggcacg ttgttacacc ccgccgcgac
     1861 ggcgccgtcg tctggagagc gtgcgccaat gatatgaact caccgggctc gagagccgtg
     1921 cgacgcatgg cctgcgagag gtggttacgc ggtgatgggc aacgatgagt tcctattgac
     1981 gagtggagca gaggagtact cgatcagcct gcgctcgagc ggcgcggctc ccccaccgcc
     2041 acccagtgaa gcgctggttt cgcgagtcgt cttcgaactg cgctacggtc tcgaggcggg
     2101 tcgcagcgag gtcgttctgc tcgcgcgccg gctcctggca gcgttggacg gaagcgggtt
     2161 cccacgttcg ctactcgacg acgcttctgc cgcggaggcg ggggagctgc tcagcctggt
     2221 cgagcgtgag attcgagcgg ggcg
//
LOCUS       DASTKS010000383         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_283556, whole genome shotgun sequence.
ACCESSION   DASTKS010000383 DASTKS010000000
VERSION     DASTKS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283556"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VFQ35_07955"
     CDS             complement(<1..1794)
                     /locus_tag="VFQ35_07955"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HET9930605.1"
                     /translation="MTLTVIGVRHHSPACARLVELRLRQLRPRFVLIEGPADMNERLA
                     ELRLAHQLPLAIFSFYQSAGRYHASWTPFCEYSPEWVALSTAQEVGAEVRFMDLPAWS
                     EAFDGVHNRYADDERRYEQALARLCKHFGAEGMDALWDHLFEQPLELEALSARLETYF
                     AGMREASATMPRDALREDYMARCLAWAAREGDVVAVCGGFHKPALERLYLEADGSAWP
                     QFPAAEADAKHGSYLVPYSFKRLDSFVGYESGMPSPEYYQAVWERGPERAAHAMLEQA
                     TRRLRKKKQVVSTADLIGASTMAAGLARLRGHAALSRCDLLDGFASAVLKSAQAAVFP
                     WNQRGKLPPGTDPILVEVVAALSGERRGQLAPNTPRPPLLVSAHAELTALDLWPEGPP
                     RKVRLKLSEPAELAKSRTLHRARVLAIPGFERSSGPTYGTEPVLEEEWSLRGAFEAES
                     ALIEASAYGATLDTAAAARLEERLGGSLDILELSALLAEATFIGIERLSASLLTTLGP
                     LVGREPDFGKLGKAQERLLALYRHDTLLGSQGSPALARVLEAAFERGLWLFEGITGPT
                     APADTALIHGVMALRDTVRFTRPRLNLDASAA"
     gene            complement(1805..2929)
                     /locus_tag="VFQ35_07960"
     CDS             complement(1805..2929)
                     /locus_tag="VFQ35_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930606.1"
                     /translation="MSKTKPTPSQSEPARKIQRPPAEELYATELAKLRETDDQPKPPG
                     WQLSLQAARRFVVGDPVAGIKRKFVGQPALVDRALVTLATNRGLMLVGEPGTAKSLLS
                     ELLAAAVSGCSTLTIQGGASVTEDHIKYSWNYALLVAEGPTPRALVPAPIHRGMSEGK
                     LVRFEEITRCPLEVQDSLLSMLSDRVLAVPELAGEPGMVFAREGFNVIATANTRDRGV
                     NEMSAALKRRFNFETVFPIPDYETELELVRSEAARLLQDSGVPHSLTPDVLEVLVTTF
                     RELRNGETQDGQAMERLSSVMSTAEAVATAHAVGVRGFYLRGNASTPADVVECLAGTA
                     AKDNADDLKRIRRYLEQRAKSKKGEHWRAFYEARKLLPGD"
     gene            complement(2966..>4120)
                     /locus_tag="VFQ35_07965"
     CDS             complement(2966..>4120)
                     /locus_tag="VFQ35_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HET9930607.1"
                     /translation="ARRLTPMIRAWPGESAHARAVVGLDVLAAIGTDVALMHLHGIAQ
                     KLKFKGLQEKAREKIDAVAEARGLTAFELADRLVPDLGLDEDTGTLTLDYGARQFVVG
                     FDEQLKPFVRDAANKRLADPPKPGKADDAEKATAALEQYKTLKKDAKAIAQSQILRLE
                     LIMCAGRRLSAEAFRNFFVAHPLMIHLVRRVLWGVYAKDGQLTSTFRVAEDRSFADSE
                     DDTFELPEDATLGVVHRLELDAATCAKWGQIFGDYEILQPFEQLGRAVFHPTPEELKS
                     DVLKRLSGVTVKTGKILGLDARGWHRGVPQDAGWVWDMYKPLGGDFRAELELKGGFCV
                     GYMEGTPAEQELGDLKLQIESGSERTKASFSELPPSVFSELVRDLEALRD"
BASE COUNT          664 a         1358 c         1345 g          753 t
ORIGIN      
        1 cgcggcgctt gcgtcgaggt tgagcctcgg tcgcgtgaag cgcacggtgt ctcggagcgc
       61 catgacgccg tggatcagcg cggtatccgc cggcgccgtt ggcccggtga tgccctcgaa
      121 cagccataac cctcgctcga aagcggcctc gagcactcgg gcgagcgccg ggctgccttg
      181 ggagccgagc agtgtgtcgt gccgatagag ggcgagtagt cgctcctgcg ctttgccgag
      241 cttgccgaag tcgggctcgc gccccacgag cggccccaag gtcgtcaaca aactcgccga
      301 caagcgctcg atcccaatga aggttgcttc ggcgagcagc gccgagagct cgaggatgtc
      361 gagcgagccg ccgagccgct cctcgagccg agctgccgca gcggtgtcga gggtggctcc
      421 gtacgcagat gcttcgatga gcgcgctctc ggcctcgaaa gctccgcgca gcgaccactc
      481 ttcttcgagc accggctcgg ttccatacgt tggcccgctc gaccgttcga agccgggaat
      541 cgcaagcacg cgtgcacgat gcagcgtgcg gctcttggcc agttcggccg gctcgctgag
      601 cttcaggcgc acttttcggg gtggcccctc cggccagagg tcgagcgcag tgagctcggc
      661 gtgggcagac accaagagcg gcggacgtgg ggtgttgggg gcgagctggc cacggcgctc
      721 gccgctgagc gccgccacca cttcgaccaa gatcgggtcc gtgcctggtg gcagcttacc
      781 tcgctgattc cacgggaaca ccgccgcttg agcgctcttc aacaccgcgc tcgcgaaccc
      841 gtcgagcaga tcgcaacgcg acagggccgc atgtccgcgc agccgcgcca agccggccgc
      901 catcgtggag gcgccgatga ggtccgcggt gctgacgact tgctttttct tgcgcaagcg
      961 gcgcgtggcc tgctccagca tcgcgtgcgc agctcgctcc gggccgcgct cccagactgc
     1021 ttggtagtac tcgggcgagg gcatgccgga ctcgtaccct acgaagctgt cgaggcgttt
     1081 gaacgagtac ggaaccaggt aactgccgtg cttggcgtca gcttctgcag ccgggaattg
     1141 gggccaagca ctgccatctg cctccaagta gaggcgctcc aaggcaggct tgtgaaagcc
     1201 tccgcacacc gccacgacgt cgccctcgcg cgcggcccaa gccagacagc gcgccatgta
     1261 gtcttcgcgg agagcgtcgc gtggcatcgt cgcggacgcc tcgcgcatgc cggcgaagta
     1321 ggtttcgagc cgtgcgctca gcgcctcgag ctcgagcggt tgctcgaaaa ggtggtccca
     1381 caaagcgtcc attccttcgg cgccgaagtg cttgcagagg cgtgccaagg cttgctcgta
     1441 ccgccgctcg tcgtcggcgt accggttgtg cacaccgtcg aaggcctcgg accacgccgg
     1501 cagatccatg aagcgcacct cggcgccaac ttcttgcgcc gtcgagagcg cgacccactc
     1561 gggcgaatac tcgcagaacg gagtccacga agcgtggtaa cgacccgcgc tctgataaaa
     1621 ggagaagatg gcgagaggca gctgatgggc gaggcgaagc tcggcgagcc gctcgttcat
     1681 gtccgcaggc ccttcgatca acacgaagcg aggtcgtagc tggcgcaggc ggagctcgac
     1741 cagccgggcg cacgccggac tgtggtgtcg gacgccaatg acggtcagcg tcacgtcggg
     1801 aacctcagtc tcccggcagg agcttgcgag cttcgtaaaa ggcgcgccag tgctcgccct
     1861 tcttgctctt cgctcgctgc tcgaggtagc gccggatgcg cttcaaatca tccgcgttgt
     1921 ctttggcagc ggtgccggcc aaacactcca cgacatcagc cggcgtgctc gcgtttccgc
     1981 gcagatagaa gccgcgtacc ccgaccgcgt gggccgtcgc cacggcttcg gcggtcgaca
     2041 tcaccgaact cagtcgctcc atggcttgcc cgtcttgggt ttctccattg cggagttcgc
     2101 ggaacgtggt gaccagcacc tcgagcacgt cgggggtgag ggagtgcggc acgccggagt
     2161 cttgcagcag ccgcgccgcc tcgctgcgca cgagctcgag ctcggtttcg tagtcgggga
     2221 tcgggaaaac ggtctcgaag ttgaatcggc gtttgagcgc cgcactcatc tcgttcacgc
     2281 cgcggtcacg cgtgttcgcg gtggcgatca cgttgaagcc ttcgcgcgcg aaaaccattc
     2341 ccggttctcc cgcgagctcc ggtacggcca agacgcggtc cgagagcatg ctgagcaatg
     2401 agtcttgcac ctcgagcggg cagcgcgtga tctcttcgaa gcgcaccaac ttgccctcgg
     2461 acatgccgcg atggatcggc gccggcacca gcgctcgcgg cgttggtccc tccgccacga
     2521 gaagcgcgta gttccacgag tacttgatat gatcttcggt cacggaggcg ccgccttgga
     2581 tcgtgagcgt ggagcaccca ctcacggccg ccgccaagag ctcggacagt agtgacttgg
     2641 cggtgccagg ctcgccgacg agcatcagcc cgcggttcgt cgccaaggtg accagcgcgc
     2701 ggtctaccag cgccggctga cctacgaact tgcgtttgat tccggcgacg ggatcgccca
     2761 ccacgaagcg ccgcgccgct tgcaacgaca gctgccaacc tgggggcttc ggctggtcgt
     2821 cggtttcgcg cagcttcgcg agctccgtgg cgtagagctc ttcggcgggt ggccgttgga
     2881 tcttgcgagc cggttcgctc tggctcgggg ttggcttcgt cttgctcatg gctctttcgt
     2941 cccaaagctc gtacctgaat ggtcgctaat cgcgaagcgc ctcgagatct cgcacgagct
     3001 cgctgaagac gctcggcggc aactcggaaa atgatgcttt cgtgcgctcg ctgccgcttt
     3061 cgatctggag cttcaagtcg ccgagctcct gttcggcagg agtgccctcc atgtacccta
     3121 cgcagaagcc gcccttcagc tcgagctcgg cgcggaagtc gccgcccagc ggcttgtaca
     3181 tgtcccacac ccagccggcg tcttgcggca cgccacggtg ccagccgcgc gcgtcgagcc
     3241 ccaagatttt gccggtcttc acggtgacgc cgctcaggcg tttgagcacg tcgctcttca
     3301 gttcctcggg ggttgggtgg aaaacggcgc gaccgagttg ctcgaagggc tgaagtattt
     3361 cgtagtctcc gaagatttga ccccacttcg cgcacgtcgc ggcgtcgagc tccaagcgat
     3421 gtacgactcc cagcgtcgcg tcttctggga gctcgaacgt gtcgtcttct gaatcggcaa
     3481 agctgcgatc ctcggcgacg cggaaggtcg acgtcaattg gccgtccttt gcgtagactc
     3541 cccacagcac gcgccgtacc agatgaatca tcagcggatg tgccacgaag aagtttcgga
     3601 atgcctcggc gctgagacgg cgacccgcgc acatgatgag ctcgagcctc aagatttgcg
     3661 actgcgcgat ggctttggcg tccttcttca acgtcttgta ctgctcgagc gcagcggtcg
     3721 ccttttcggc gtcgtctgct ttcccgggct tgggcggatc ggcgagccgc ttgttcgcag
     3781 cgtcgcgcac gaagggtttt agctgttcgt cgaatccgac gacgaactgc cgcgcgccgt
     3841 agtccaacgt gagcgtaccg gtgtcttcgt ccaacccgag gtcgggcacg aggcgatccg
     3901 ccagctcgaa tgcggtgagc ccgcgcgcct ctgccaccgc gtcgatcttc tcgcgcgcct
     3961 tctcctggag gcctttgaac ttgagcttct gcgcgatgcc gtgaagatgc atcagcgcca
     4021 cgtcggtccc gatggctgcg agcacgtcga ggcccacgac cgcgcgggca tgagcacttt
     4081 cgccgggcca ggcgcggatc atcggcgtga gtcggcgcgc
//
LOCUS       DASTKS010000384         9669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1846373, whole genome shotgun sequence.
ACCESSION   DASTKS010000384 DASTKS010000000
VERSION     DASTKS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846373"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFQ35_07970"
     CDS             complement(<1..392)
                     /locus_tag="VFQ35_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9930608.1"
                     /translation="MNNLSNSSSQPAAEKSPSRPRILFVVDGFYPATGGAEKQVRTLA
                     KAFAAEGHAVEVVAPQLDPSLPTLDDVDGIVVTRLAYPHIKLLGAALLCARFGYWVWN
                     RRGDFDAIHVNMAKNLAAVAGFLRPALPS"
     gene            complement(503..1951)
                     /locus_tag="VFQ35_07975"
     CDS             complement(503..1951)
                     /locus_tag="VFQ35_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9930609.1"
                     /translation="MNDLANKTYDAVVVGTGIGGATFAYALAKRGLNVLVAERGDYFK
                     PERKDLAPLHLHHFAKLSVIGGQTKSFGAAMYRLRESDFSPVEMEAGISPGWPITYAD
                     LEPYYCEAERLFKVHGSSENDSTEPPRSQPWPFPAIPHQGPARELVERVSERAKVSVS
                     YIPRGIDYDPNNGGKCVLCRHCDAYYCPRDAKMDAEIAALRPAVDTGRVTVLTNTECV
                     RVLTTPDGKRVSGINLRKDGREFTVNTSLVGLGSGLRDTPVILWRSRTSQHPKGLSNG
                     SGALGRYWAAHTQGWVFPIVLGVQKREFHQKTFAINAFYDSGPDWKYPLGTIQASGNI
                     EPIGMSRRYRYPAELIIEHSFQTFVMSEALPTINTGFVLSDDGSKVLSEPIKNKRTFA
                     KLRRYATKIFRSAGYVVFAPPMEANFHNVGTARMGSDPATSVINGDCKAHDVDGLYVV
                     DASALPTSGALNSGLSIAAVALRAAASVPLAG"
     gene            complement(2039..3118)
                     /locus_tag="VFQ35_07980"
     CDS             complement(2039..3118)
                     /locus_tag="VFQ35_07980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9930610.1"
                     /translation="MDSRKVSIVIPTYNRAYCLAATLESLQRQTYPHWEALIIDDGST
                     DDTEALVASIQSSDPRIRYVRKENGGVSSARNTGIRLADGDWVAFLDSDDAWEPWKLE
                     AQLACTKELPDVGMVWTDMNAFDAEGNLVSPRHLRKMYTAYARVGEHRLFSAHRKVRD
                     FAPELTSHGPLADATVRWGEISSWMIFGSLVHTSTVLISRERLRAVGFFNEKYRTGED
                     YEFHLRTCFEGPVALLDAPSIRYRVAGGADQLTAPKHRLEIALNGLRIREAAIAANRA
                     RINLSEAELAGIMARANAFVAEELFQQGDFERARAHFRRSGLLRFRNFNRLLKASIAH
                     LPAGMAKPVVELIRGGKVKAKGWRS"
     gene            complement(3530..4738)
                     /locus_tag="VFQ35_07985"
     CDS             complement(3530..4738)
                     /locus_tag="VFQ35_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930611.1"
                     /translation="MSTGLLLAAVGFLGTPALPQSMANAGARLEARVRQTPVGAGERR
                     LALDLDATATGDISFTWRRTSLGFGYAPRFTVTDVFDRGSKDLLHNAGINFGWQGRRL
                     SLGVSEYASYGRRWFSLTEPTATNPMTGLPVITAIPAPTSVLFVSLQTTLSARYQLSR
                     RSILGASATYGISGGVDEESRRAVPLLKGPSATLQLEYHATRSDTVGPELSVAYTRAT
                     IAQPEDRTIVSGYVSGLGFWSRRWSHRVSSRLAGGVTVQRRFEPTQRVKVFPSGGAGF
                     DYTVPWGGNHWMATFSANATLGSVVNMLTGELQQTASAFFLASWTRFRWTLHTSVGAL
                     RPIGRAGGDITLVFADAGSHWGFTRALGWDVGIRAGTQSTGRSEIPAASGTQIGVFTA
                     LSFTMPPHRL"
     gene            complement(4735..5298)
                     /locus_tag="VFQ35_07990"
     CDS             complement(4735..5298)
                     /locus_tag="VFQ35_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9930612.1"
                     /translation="MLLAAYASGCADTGQYVWARELPQQARVDQGYIIRTGDQLDVRV
                     YNEDRLAGRPRVRSDGFITLPLLGDVLARGKTPAGLARDLTALLSKYLNAPTVTVGVD
                     DSRPVTITMIGEFSRPGIYTLNPGSGLVQALALAGGFNDFADRDALFVMRTAPQQRVR
                     FRYKDLLNNDPSTVGFQLLDGDVVAAE"
     gene            complement(5407..6363)
                     /locus_tag="VFQ35_07995"
     CDS             complement(5407..6363)
                     /locus_tag="VFQ35_07995"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9930613.1"
                     /translation="MKILVTGGAGFIGSHIVEALLALGHEVAVLDNLSSGKRENVPAN
                     VRLFEVDLRDRDATFKAMADFRPNAVSHQAAQASVSISMREPKLDAEVNVIGGINLLD
                     ACTAPGAAVERFVFASTGGAIYGEVPPGTSASESTQPRPASPYAIHKLTLEHLLSVYR
                     ARDGLNVTTLRYANVYGPRQDPHGEAGVVAIFFNAILEGRKIRVNARAEAGDRGCLRD
                     YIFVSDVARLNVLGITGGLRDTVVNAGTGKETDTQSLAEMIMRVTGRTVPVEFGAVRA
                     GDLQRSVLDPRLTEQYLGSLVDLDRGMRGTFDFYSRRSGTGG"
     gene            complement(6636..7352)
                     /locus_tag="VFQ35_08000"
     CDS             complement(6636..7352)
                     /locus_tag="VFQ35_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930614.1"
                     /translation="MDERTLTIGGLRTVVVRSEQPRAIVVVLHGFAMQPSDLSPFAHS
                     LNVPGSFFFPEAPHAAEIAPEDWRGRAWWHIDPVGRAAALARGPRDFCAEHPEGLPYA
                     RRVLADFLSAVRRFDAALEGAPLILAGFSQGGMLACDALLRGEIEVDGLALFSASRIA
                     FDEWQPLLAGAARARSFPPLLISHGMSDDDLAFSAGVALRDCLEAVGAAPTFVSFPEG
                     HVIPMVVWRAFRKFIKSLAR"
     gene            complement(7353..8501)
                     /locus_tag="VFQ35_08005"
     CDS             complement(7353..8501)
                     /locus_tag="VFQ35_08005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9930615.1"
                     /translation="MTAPRAEPAPRRAPEAARAALAGHLPSLDGVRGLAILLVLFHRF
                     DLYPETSSVSRVLARLCDLGWVGVQLFFVLSGFLITGILLDSKGSANYFAAFFMRRAL
                     RILPLYYATLVVGLILVPLAMGEPSRAGDEPLLWCFLSNWPTQEHSVFPHFWSLAVEE
                     QFYWVWPFVVYALDARKLSWLCGALAAGALVVRIGVLAAGLNPEQVYTWTICRMDALA
                     NGAALAALFRMSATREWLARFRSWIAAATLALVLAAFVLTNRYERIGSAGQTLGYTVL
                     ALAFTVLVGAAALGEIEKRGFAVRFFSLPPLRTLGKYSYAAYIFHVAIHHTLVKYWVR
                     IEREPTLLGALGYAAVGIVLSLLAAFASYQLFERHFLALKRRFAVRAH"
     gene            complement(8498..>9669)
                     /locus_tag="VFQ35_08010"
     CDS             complement(8498..>9669)
                     /locus_tag="VFQ35_08010"
                     /inference="COORDINATES: protein motif:HMM:NF036482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HET9930616.1"
                     /translation="PLVHAVLPALVTEFKGDTVYWPSSAHGGEFPHVCWSGTTSYYGV
                     GAYLRPLEDARRSEVRFASECLAFANVPHPEALPGGPSARVHHPAWKARTPRDLGAGW
                     DFDDVRDHYVERLFGVDTRALRYGDHDRYLELGRAATGEVMAATISEWRRSRSQCRGA
                     LIWFLKDLWPSAGWGIFDSSGAPKSVYYYLRRVMQPLFLGFSDEGLNGLSLHAVNETP
                     RALAGEIRVRFFRHFETLSSELAKPLELPAHGARELPLGAWLEGFSDLTYAYRFGPPS
                     VDLVTASLEVDGASRATAFYFPLGFPRERETELGLSARGRALGDGRFEVVVQTKRFAQ
                     AVTLNVAGATADDDYFNVAPGATKTIVLTLGEPRPSLRGSVQALNAVAPVPIRFE"
BASE COUNT         1849 a         3168 c         3156 g         1496 t
ORIGIN      
        1 gagggcagcg cgggccgcaa aaacccagcc acagcggcca agttcttcgc catgttcacg
       61 tgaattgcgt cgaaatcacc gcgccgattc caaacccagt agccgaaacg agcgcacagc
      121 agcgcggccc cgagcagctt gatgtgaggg tacgcgagcc gagtcaccac gatcccgtcg
      181 acgtcgtcca acgtcggaag gctcgggtcg agctgcgggg ccacgacctc cacggcgtga
      241 ccctccgccg cgaacgcttt ggcgagcgtc cgtacctgct tctccgcgcc gccagtggcg
      301 ggataaaagc catcgacgac gaataggatg cgcggccgcg acggcgactt ctcagcggcc
      361 ggctgggagc tcgagttcga caggttgttc atcgtgcagg gctcggttga gcggcgcggg
      421 cgaaccgcgc ctttggaccg tatagacgac ggctcctcaa actcaagcgc cggacgaggg
      481 gccgcaggaa ttcggctcca ccctagccgg cgagcggcac cgacgctgcc gcgcgtagag
      541 cgaccgcggc gatgctcaaa ccgctattca gcgctcccga cgtcggaagc gcgctcgcgt
      601 cgacgacgta gaggccgtcc acgtcgtgag ccttgcagtc gccgttgatg accgaagtcg
      661 ccggatccga acccatgcgc gccgttccca cgttgtgaaa attcgcttcc atcgggggcg
      721 cgaacaccac atagcctgcg gatcggaaga tcttcgtcgc gtagcggcgc agcttcgcga
      781 aggtccgttt gttcttgatc ggctcggaca acaccttgga gccgtcgtcg ctcaacacga
      841 acccggtgtt gatggtgggg agagcctcgc tcatgacgaa cgtctggaac gaatgctcga
      901 tgatgagctc ggctgggtac cgatagcgcc gcgacatccc gatgggctcg atgttgccgg
      961 acgcttgaat ggttccgagc ggatacttcc aatccggacc ggagtcgtag aaggcgttga
     1021 tggcgaaggt cttctgatga aactcacgct tttgaacgcc cagcacgatc gggaagaccc
     1081 agccttgcgt gtgcgcggcc caatagcggc cgagtgctcc ggacccgttc gagagcccct
     1141 tggggtgctg gctcgttcga gaacgccaga ggatgacggg cgtatcgcgc aacccgctcc
     1201 ccagcccgac gaggctcgtg ttcacggtga attcacgccc gtccttgcgc agattgatac
     1261 cgctcacccg ctttccgtcg ggagtcgtga gcacgcgaac gcactcggtg ttggtgagca
     1321 ccgtgacgcg acccgtgtcg acggcggggc gcagcgccgc gatctcggcg tccatcttgg
     1381 cgtcgcgcgg acagtagtaa gcgtcgcagt gccggcacag gacgcacttt ccgccgttgt
     1441 tcgggtcgta atcgataccg cgcggaatgt aagagaccga aactttcgcg cgctcgctca
     1501 cgcgctcgac gagctcgcga gccgggccct gatgaggaat cgccgggaag ggccagggct
     1561 gggagcgagg tggctcggtc gaatcgttct cggaagagcc gtgaaccttg aagaggcgct
     1621 cggcctcgca gtagtacggc tccaaatcag cgtacgtgat cggccaaccc ggcgatatgc
     1681 ccgcctccat ctcgacgggt gagaaatccg attcgcgcag ccggtacatc gccgcgccga
     1741 acgacttggt ctggccacca atgaccgaga gcttggcaaa gtgatgcaaa tgcaagggcg
     1801 cgaggtcctt gcgctcgggc ttgaagtagt cgccgcgctc ggccacgagg acgttcagtc
     1861 ctcttttcgc gagcgcgtac gcgaacgtcg cgccgccgat ccccgtcccc accaccaccg
     1921 catcgtaggt tttgttcgca aggtcattca tcgcgtcgat ctcttttcaa gaaagcgctc
     1981 gagcgctgtc acggcagacc gggagtcacg agccacgtac gcccgccgac aggctacttc
     2041 aactccgcca tcccttggcc ttcaccttac cgccgcggat gagctccacc accggcttcg
     2101 ccatccccgc tggcaagtgc gcgatcgacg ccttcaacag ccggttgaag ttgcggaagc
     2161 gtaagaggcc gctgcgacga aagtgagcgc gcgctcgctc gaagtctccc tgctgaaaga
     2221 gctcttctgc gacgaacgca ttggcccgcg ccatgattcc ggcaagctcc gcctccgata
     2281 ggttgatgcg cgcgcggttc gcggcgatgg cggcctcgcg gatcctgagg ccgttcaacg
     2341 cgatctcgag gcgatgcttg ggagcggtga gttgatccgc tccgcctgcc actcggtagc
     2401 ggatcgaggg agcgtccaac aacgcgaccg gcccctcgaa gcacgtgcga aggtggaact
     2461 cgtaatcttc cccggtgcga tacttttcgt tgaagaaacc gaccgctcgc agccgctcac
     2521 gcgaaatgag cacggtggac gtatgcacta ggctgccgaa aatcatccac gacgagatct
     2581 caccccatcg caccgtggcg tcagcgagcg ggccgtgcga cgtgagctcc ggcgcgaaat
     2641 cgcggacctt gcggtgggcg ctgaacagcc ggtgctcacc gacgcgcgcg tacgccgtgt
     2701 acatcttccg aaggtgccgc ggcgacacga ggttcccctc cgcgtcgaac gcgttcatgt
     2761 cggtccaaac catgccgacg tcgggaagct ccttggtgca cgcgagttgc gcttctaact
     2821 tccaaggctc ccaagcgtcg tccgaatcga ggaacgcgac ccagtcgccg tccgcgagac
     2881 gaataccggt gttcctcgcc gacgacacgc cgccgttctc ctttcgaacg taacgaatcc
     2941 gcggatcgct cgactgaatg gatgcgacga gcgcctctgt gtcgtcggtg gatccatcat
     3001 cgatgatgag agcttcccaa tgcggatacg tctgccgttg caaactctcg agagtcgccg
     3061 caaggcagta agctcggttg taggtgggaa tgacgatgct tactttgcgg ctatccatcg
     3121 acaccaggtt tggcggacct tcgttcaagc ttactccact gcccacctga agacgaaaca
     3181 atatttaatc cgtcactgca acgcacctac tacgcacgca agtttctgaa acatcctcgc
     3241 tttcctcgcg cgggcgggag cgcgaacacg ggagcgatca cgccgacgcg agacaacgga
     3301 tgacggtcct ttgcgtctcg gaacgagccg gcgagagcga ctgttcagga gcgcccgacg
     3361 caagccgagc ggaggcatta cggtcgcgta aggttccgcg aaatggacga aaccggtgcg
     3421 aacgccaccg agcttacgag acgtaaggaa ccgtcagtgg ccgtgctcgg ggcgctcgcc
     3481 atccgcgcga cgctggcggc tttgcgtcac ggccgcccgt cggcgcgatt caaaggcgat
     3541 gcggcggcat cgtgaaactc agcgcggtga agacgccgat ctgggtgccc gaagcggcag
     3601 ggatttccga gcgaccggtc gattgggtcc ccgctcgtat gccgacgtcc caaccaagcg
     3661 ctcgcgtgaa gccccagtgc gagcccgcgt cggcaaatac cagcgtgatg tcgccgccgg
     3721 ctctcccgat tggacggagc gcgccgaccg aagtgtgaag agtccagcgg aaccgagtcc
     3781 aactcgccag gaagaaagcc gaagccgtct gctggagctc gcccgtgagc atgttgacga
     3841 ccgagccgag cgtcgcgttg gcgctgaagg tcgccatcca gtggttaccg ccccacggaa
     3901 ccgtatagtc gaagccggcg ccgccgctcg gaaacacctt cacgcgctgg gtgggctcga
     3961 accgacgctg caccgtaacg ccgccggcga gccgagacga cactcggtgc gaccagcgtc
     4021 gcgaccagaa gccgagcccg gagacgtagc cggacacgat ggtgcggtcc tcgggctgag
     4081 cgattgttgc gcgcgtgtac gcgaccgaga gctctggacc aaccgtgtcg ctgcgagtcg
     4141 cgtgatactc gagctgcagc gtcgccgacg gccccttcaa gagcggcacg gcccggcgcg
     4201 actcctcgtc gacgccgccg ctgataccgt aagtggccga cgcgccgagg atcgagcgcc
     4261 gcgagagctg atagcgcgcg gagagcgtcg tctgcaacga gacgaacaag acgctggtgg
     4321 gcgccgggat cgccgtgatc accggcaggc ccgtcattgg gttcgtcgcc gtcggctcgg
     4381 tcagcgaaaa ccagcgccgc ccgtaggacg catactccga aacgcccaag ctcagccgcc
     4441 gtccctgcca gccgaagttg attccggcgt tgtggagcag gtccttcgag ccgcggtcga
     4501 agacgtcggt caccgtgaag cgcggcgcat agccgaaccc caagctggtg cggcgccacg
     4561 tgaagctgat gtcaccggtg gcggtcgcgt ccagatcgag cgcaaggcgc cgttctcccg
     4621 cgcccacggg cgtctgcctc acgcgcgcct cgagccgagc tcccgcgttg gccatgcttt
     4681 gcggtagagc gggcgtcccg aggaagccca ccgcggcaag caagagcccg gtgctcactc
     4741 ggccgcaacc acgtcaccgt ccaacagctg gaagccgacg gtactcggat cgttgttgag
     4801 caaatccttg tagcggaagc gcacgcgctg ctgcggggcc gtgcgcatca cgaagagcgc
     4861 gtcgcgatcc gcgaagtcgt tgaagccacc ggcgagcgcg agcgcttgaa cgagcccgga
     4921 acccgggttc aaagtgtaga tgccggggcg gctgaactca ccgatcatcg tgatggtgac
     4981 cgggcgcgaa tcgtcgacgc ctaccgtgac ggtcggcgca ttcaggtact tggaaagcag
     5041 cgcggtgaga tcgcgcgcca agcccgccgg cgtcttgcca cgggccaaaa cgtcgccgag
     5101 gagcggcaac gtgatgaagc cgtcgctacg gacccgcgga cgcccagcca agcggtcctc
     5161 gttgtacacg cgcacgtcga gctgatcgcc cgtgcgaatg atgtatccct gatcaactcg
     5221 tgcttgttgc ggcagctcgc gagcccacac gtattgcccc gtgtccgcgc accccgaagc
     5281 atacgcggcg agcaacaaca gtagccctcg acccagacca cgccgacaaa ggctcataat
     5341 cgggaatcat ggacgcgctc gccgtcgagg tcaatcggtg cggcggctcg atgagtgact
     5401 tctaaatcag ccgcccgtcc cggaccttcg cgagtagaag tcgaacgtgc cgcgcatgcc
     5461 gcggtcgagg tcgacgagcg agcccaaata ctgctcggtg aggcgtggat cgagcacgga
     5521 gcgctgcaaa tcgccggcgc gaacggcgcc gaactcgacc ggcacggtgc gacccgtgac
     5581 acgcatgatc atctccgcga ggctctgcgt gtctgtctct ttgccggtgc cggcgtttac
     5641 gacggtgtcg cgcagcccgc cggtgatgcc gagcacgttc agacgcgcca catcagagac
     5701 aaatatgtaa tcgcgcaggc acccgcggtc tcccgcctcg gcgcgcgcgt tcacccgaat
     5761 tttacggccc tcgaggatcg cgttgaagaa gatcgccacg acgccagcct cgccgtgcgg
     5821 gtcttggcgt gggccgtaga cgttggcgta gcgcagggtc gtcacgttga gcccgtcacg
     5881 cgcgcgatag accgacaaga ggtgctcgag cgtgagcttg tggatcgcgt acgggctcgc
     5941 cgggcggggc tgcgtgctct ccgacgcgga cgtgccgggt gggacctctc cgtagatggc
     6001 gccgcccgtg ctcgcgaaca cgaagcgctc gacggctgct ccgggcgccg tgcacgcgtc
     6061 gagcaagttg atgccaccga tgacgttcac ctctgcgtcg agcttcggct cgcgcatgct
     6121 gatggagacg ctcgcctgcg cggcctgatg gctgaccgcg ttgggacgaa agtctgccat
     6181 tgccttgaac gtggcatcac gatcacgcag atcgacctcg aacaggcgaa cgttggcggg
     6241 aacgttctcg cgtttgccgg agctcaagtt gtcgagcacc gcgacttcgt gtccgagcgc
     6301 aagcagagcc tcgacgatgt ggctcccaat gaaaccggca ccaccagtga cgagaatttt
     6361 cacatcattc agatagcaaa gtctcgaacc cgagcgccag cgagatcgca cgaggcgcgc
     6421 cgacaccccg gctctgatgt gccttttgtt acgcgagcgc tcctttcgcc gtcgccgcca
     6481 ctccgcgcgc ccgccgagtt gccggagggt cgcagattcc cgggctgcga gatccgaatc
     6541 gcgcggagcg agccgcgcag cgaagccgac cgtcagcgcg atgattacga gattccaggg
     6601 accggagtgg cctcggtgcg cgaggcgacg ctacgtcacc tcgcgagcga cttgatgaac
     6661 ttgcggaacg cgcgccaaac caccatcggg atcacgtgac cttccgggaa agacacgaag
     6721 gtcggtgctg cgccaacagc ctcgagacaa tcgcggagcg caacccccgc gctgaaggcg
     6781 agatcgtcgt ccgacatccc gtgactgatg aggaggggtg gaaacgaccg cgcacgcgcc
     6841 gccccagcga gtaagggctg ccactcgtcg aacgcgatgc gtgatgcaga gaacaaggcg
     6901 agaccgtcca cctcgatctc accgcgcaac agcgcgtcac atgccaacat gccgccttga
     6961 gaaaagccgg cgaggatgag cggcgcccct tcgagcgcgg cgtcgaatcg acgcacggcg
     7021 ctcaagaagt cagcgagcac tcgcctcgcg tacggcaacc cctccgggtg ttcggcgcaa
     7081 aagtcgcgag ggccccgcgc gagcgccgcc gcgcggccga ccgggtcgat gtgccaccag
     7141 gcccggcctc gccaatcttc gggcgcgatt tctgcggcgt gaggcgcttc gggaaaaaag
     7201 aacgagccgg gcacgttgag cgaatgagcg aacggcgaga ggtcggacgg ctgcatcgcg
     7261 aagccgtgga gcacgaccac gatcgcgcgc ggctgctcgc ttcgaacgac cacggtgcgc
     7321 aagccaccga tcgtgagggt gcgctcgtcc attcagtgcg ctcgcacggc aaagcgcctc
     7381 ttcagcgcga ggaagtggcg ctcgaagagc tggtacgagg cgaacgcagc gagcagcgaa
     7441 aggacgatcc cgaccgccgc gtacccgagc gccccgagca acgtcggctc gcgttcgatc
     7501 cgaacccaat actttaccaa cgtgtgatga atcgcgacgt gaaagatgta cgccgcatag
     7561 ctgtatttgc ccagcgtgcg caacggcggg agtgagaaga agcgcacggc gaagcctcgc
     7621 ttttctatct ctcctagcgc ggcggccccg acgagcacgg tgaacgcgag cgcgagcacg
     7681 gtgtagccga gcgtctgtcc ggcgctcccg atgcgctcgt agcggttcgt cagcacgaac
     7741 gcggcgagga ccagggcgag cgtcgcggcc gcgatccacg agcggaagcg cgcgagccat
     7801 tcgcgcgtcg cgctcatgcg aaacagcgcc gccagggccg cgccgttagc gagcgcgtcc
     7861 atgcggcaaa tcgtccaagt gtagacttgc tctgggttca gcccggccgc cagcacgccg
     7921 atgcgcacga cgagcgcgcc cgccgcgagc gcgccgcaaa gccacgaaag cttgcgcgcg
     7981 tcgagcgcgt acaccacgaa cggccacacc caatagaact gctcttccac cgcgagcgac
     8041 cagaagtggg gaaacacgct gtgctcttga gtcggccagt tcgacagaaa gcaccagagc
     8101 agcggctcgt cgccggcgcg agatggctcc cccatcgcga gcggaacgag gatgagcccg
     8161 accacgagcg tcgcgtaata gaggggcaga atgcggagcg ctcggcgcat gaagaacgct
     8221 gcgaagtagt tggcgctacc cttcgaatcg agcaggattc cggtgatcag gaacccggac
     8281 agcacgaaga agagctggac gccaacccag cccaagtcgc aaaggcgtgc gagcacgcgg
     8341 ctcacgctgc tcgtctccgg atagagatcg aagcgatgaa acaaaacgag caagatggcg
     8401 agcccacgga cgccgtcgag gctcggcagg tgcccggcca acgccgctct cgcggcctcg
     8461 ggcgcgcggc gcggcgcggg ctccgctctc ggcgccgtca ttcgaaccgg atggggacgg
     8521 gggctacggc gttgagcgcc tggaccgaac cgcgcagcga cgggcgcggc tccccgagcg
     8581 tcagcactat cgtcttcgtc gcgcccggcg ccacgttgaa gtagtcatcg tcagcggtgg
     8641 ctcccgcgac gttgagcgtc accgcctgcg cgaaacgctt ggtttgcacc acgacttcga
     8701 agcgaccgtc accgagcgcg cggccccgag ccgacagccc gagctccgtc tcgcgctcac
     8761 gagggaagcc gagcgggaag tagaatgcgg tcgcgcgcga ggctccgtcg acttcgagcg
     8821 acgccgtgac gaggtcgacg ctcggcgggc cgaagcggta ggcatacgtg aggtcgctga
     8881 agccctcgag ccacgcgccc aggggaagct cacgcgcgcc gtgcgccggc aactcgagag
     8941 gctttgcgag ctccgagctc aacgtctcga agtggcggaa gaagcgaacc cgaatttctc
     9001 ccgcgagcgc gcgcggcgtt tcgttgacgg cgtgtaacga gagcccgttg agcccctcgt
     9061 ccgaaaagcc caaaaacagg ggctgcatca cgcgacggag atagtagtag accgacttcg
     9121 gcgcgccgct cgaatcgaag atgccccaac ccgcgctcgg ccaaaggtcc ttcaaaaacc
     9181 agatcagcgc cccgcgacac tgggaacggc tccggcgcca ttcggagatg gtcgcggcca
     9241 tgacctcacc cgtcgccgcg cgtccgagct ctaggtagcg atcgtggtcg ccgtaccgca
     9301 gcgcgcgcgt gtcgacgccg aacaagcgct cgacatagtg atcgcgcaca tcgtcgaagt
     9361 cccaacccgc tccgaggtct cggggcgtgc gcgctttcca cgcggggtgg tgtacccgcg
     9421 ccgaagggcc gccgggcagc gcttcggggt gagggacgtt cgcgaaggcg agacactcgg
     9481 aagcaaagcg aacctcggag cggcgcgcgt cctcgagcgg tcgcaagtag gcgccgacgc
     9541 cgtagtacga cgtggttcca gaccaacaga cgtgcgggaa ttcaccgccg tgcgcgctcg
     9601 aaggccaata cacggtgtcg cccttgaact cggtgacgag cgcgggcaaa accgcgtgaa
     9661 ccagcgggt
//
LOCUS       DASTKS010000385         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_283737, whole genome shotgun sequence.
ACCESSION   DASTKS010000385 DASTKS010000000
VERSION     DASTKS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283737"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2924)
                     /locus_tag="VFQ35_08015"
     CDS             complement(<1..2924)
                     /locus_tag="VFQ35_08015"
                     /inference="COORDINATES: protein motif:HMM:NF037694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9930617.1"
                     /translation="MRSFRFGGVTTGTVLVGAFAIVSCLRARPPDVKPGSTLGTSAHG
                     GAARQGPFAVVYAGPRDKAAPTDQAVTVLFNRPMRTFEAADTAGLPPVSIRAEDGRVV
                     PGTWRFVGTHGLLFTPQAPFPGSTRFQVTVAATARSLAGDTLPSDYTFSFSTDPPAVT
                     SSWPRESARDLRSDSKIRLIFNQRVDPDAVALKARLVVRAKPGDAPRSIALRGARATQ
                     RVPSDGNAKLERIVVLTPVEALPLDNDVRLVLDAGLSGEGPLPSKTPFVLETRTYGPL
                     RLADFRCPRVTPGRCQAHRDISIVLTNDVAPAEFKAHLKATALPLQKVATASTGKPDN
                     SPAARAARAKAQEPRREHLLGADPEMGKRYRVTLTKGMHDVFGQALAQDVSLEVDIEA
                     PFVTADGKQKIDPKVASGASPESESVPAEDQGEDSDSDEVSVEAAAKRDKRPRRPVLS
                     YELALGVRGHVVEAVRKDGARPHIVPIGSVNLATYGLFATKMRREDAVVWLGAKPRSF
                     DDPLRWTWITPGVPENTRAVKNVDLDALLGGADARGAALLAVGWPGSANSAHQSELLT
                     VTDIGLSAAMSSYGSMVWVTRLSTGQPLPGAVVSVQRPTDQREAFRGTTDEQGVVTIP
                     KQAYDPIKNGNIDHGAVLFASLGEDWTYQRVERAMASARSGVPIDLQQRGEWAGMLYT
                     DRGVYRPGESLKLAGIFRKFDAAGMKTPPGAEARIEISDSQGEHLFEGRAKLDAFGGV
                     AVDVPLPKTSHLGDAYISASFGKANTNTFTSQVLLAAYKASEFKVAVEPGKSEYVRGD
                     EAPFDLHAEYLFGAPMGNATFRTTARRNTTSFVPPGADAYAVSDEASVLDAVASNPRT
                     EELSDEDGTLDDAGAARRTLKLELPRMAGPEQVTLEAEVRDLTNQTVARRATVLVHPA
                     AFYLGLKRLERRFLAVGAELPATVVAFDPKGAHVPGVTSKVELIRRTWVGVVEDEAAE
                     AP"
     gene            3433..3840
                     /locus_tag="VFQ35_08020"
     CDS             3433..3840
                     /locus_tag="VFQ35_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9930618.1"
                     /translation="MEFHRGRLIDHVHLRVADVEASKRFYRAVFASLGLPDIVHEGPG
                     FFYADELFVDKADGYTSRVHLAFQAADRATVQSFHRAALAAGGKDNGGPGERSYHPGY
                     YGAFVLDPDGNNIEAVFHGPNERSAPSVIVTAK"
BASE COUNT          624 a         1259 c         1315 g          714 t
ORIGIN      
        1 tgcggcgctt cggcggcctc gtcttcgacg acgccgaccc acgttcggcg gatgagctcg
       61 accttggaag tgactccggg cacgtgcgcg cccttggggt cgaaagccac gaccgtcgcc
      121 ggaagctcgg ctccgaccgc gaggaaacgc cgctcgagcc gcttcaaccc gagatagaaa
      181 gcggcgggat gcacgagcac cgtcgcgcgg cgggcgacgg tctggttcgt gaggtcacgc
      241 acctcggctt cgagcgtgac ttgctccggc ccggccatgc gcggcaattc gagcttgagt
      301 gttcgccgcg ccgcgccggc gtcgtcgagg gttccatctt cgtccgagag ctcctcggtt
      361 cgcgggttgc tcgcgaccgc gtcgagcacc gacgcttcgt ccgacacggc gtacgcatcc
      421 gcgccgggcg gaacgaagct cgtcgtgttg cgccgcgcgg tcgtgcgaaa cgtggcgttg
      481 cccattggcg ccccgaacaa atactctgcg tgcaaatcga acggcgcctc gtctccgcga
      541 acgtattcgc tcttgccggg ctctaccgcg accttgaact cgctcgcttt gtaggccgcg
      601 agcagcacct gcgacgtgaa agtattggtg ttcgccttgc cgaaggacgc gctgatgtaa
      661 gcgtcgccca ggtgactcgt cttcggcagc ggaacgtcga ccgcgacgcc gccgaaagca
      721 tcgagctttg ctctgccttc gaacaggtgc tctccctgcg aatccgagat ctcgatgcgc
      781 gcctccgcgc ccggcggcgt tttcatgccc gccgcgtcga acttgcgaaa gatcccggcg
      841 agcttcagtg actcgccggg ccgatacacc ccgcggtcgg tgtagagcat gcctgcccat
      901 tcgcctcgct gttggaggtc gatgggaact cccgagcggg cgctcgccat ggcgcgttcg
      961 acgcgctggt acgtccagtc ttcgcccaag ctcgcgaaca gaaccgcgcc gtggtcgatg
     1021 ttgccgttct tgatggggtc gtatgcttgc ttggggatcg tgacgacacc ttgctcgtcg
     1081 gtcgtgccgc ggaaagcttc gcgttggtca gtggggcgtt gcacgctgac gacggcgccc
     1141 gggagcggct gccccgtaga aagccgagtc acccaaacca tgctcccgta gctgctcatg
     1201 gccgcgctga gcccgatgtc ggtgacggtg agtagctcgg actggtgcgc gctgttggcc
     1261 gagccgggcc agccgaccgc gagcagcgcc gccccgcgcg cgtccgcgcc gccgagcaaa
     1321 gcgtcgagat cgacgttctt caccgcgcgc gtattctctg gcacgccggg agtgatccaa
     1381 gtccaacgca gcgggtcgtc gaacgaccgc ggctttgcac cgagccacac caccgcgtct
     1441 tctcgccgca tcttggtcgc aaacaggccg tatgtcgcca ggttcacgga gccgatgggg
     1501 acgatgtgcg gcctcgctcc gtctttacgg accgcttcga cgacgtgtcc tcgtacaccg
     1561 agcgcgagct cgtacgaaag caccgggcgg cgcggccgct tgtctcgctt cgccgcggct
     1621 tcgacggaca cttcgtcact gtccgagtct tctccttggt cttcggcggg caccgattcc
     1681 gactcggggg aggcgccgga ggcgaccttt gggtcgatct tctgtttgcc atcggccgtg
     1741 acgaacggcg cttcgatgtc gacctcgagc gacacgtctt gcgcgagtgc ctgcccgaac
     1801 acgtcgtgca tccctttagt gagcgtgacg cggtagcgct tgcccatctc ggggtctgcg
     1861 ccgagcagat gctctcgccg tggctcctga gctttggcgc gcgcggcgcg ggcggcaggg
     1921 ctattgtcgg gtttcccggt gctcgcggtc gcgaccttct gcagtggcag cgcagtcgcc
     1981 ttgagatgag ctttgaactc cgcgggcgcg acgtcgttcg tgagcacgat gctgatgtcg
     2041 cggtgggcct gacagcggcc cggggtcaca cgagggcaac ggaaatcggc gaggcgcaga
     2101 ggcccatagg tgcgtgtttc gagcacgaac ggtgtcttgc tagggagcgg tccttctccg
     2161 ctgaggcccg cgtcgagcac gagccgaacg tcattgtcga gcgggagcgc ttcgaccggc
     2221 gtaaggacga cgatccgctc gagcttcgcg tttccgtcgc tcggcacgcg ctgagtcgcc
     2281 cgagcgccgc ggagcgcgat cgatcgcggc gcgtcgcctg gcttggctct gacgacgagc
     2341 ctcgctttga gtgcgaccgc gtccggatcc acgcgctggt tgaagatcag gcggatcttc
     2401 gagtcgctgc gcaagtcgcg ggcgctctcg cgtggccagg agctcgtcac cgcgggcgga
     2461 tcggtagaga acgaaaaggt gtagtcgctc ggcaaggtgt ctccagcgag cgagcgcgcg
     2521 gtagctgcga ccgtgacttg aaatcgcgtc gagcctggaa acggcgcctg cggtgtgaag
     2581 agcagcccgt gcgtgccgac gaaacgccaa gtgcccggaa cgacgcgtcc gtcttccgct
     2641 cgaatcgaaa ccggtggcaa ccctgccgta tccgccgcct cgaacgtgcg catggggcgg
     2701 ttgaacagca ccgtcaccgc ttggtcggtt ggcgcggcct tgtcgcgcgg acccgcgtac
     2761 accacggcga atggaccttg gcgcgccgca cccccgtgcg cgctcgttcc caaggtggaa
     2821 ccgggtttca cgtccggcgg acgagcccgc aaacagctga cgatcgcaaa cgcgccgacc
     2881 agcaccgtcc cggtcgtaac accaccgaat cgaaacgaac gcatcagagc ctcccgagcg
     2941 cctcacacat accaggacgg agcagaaagt gtggcggaag aacgctgctc ggcggctttc
     3001 gcttcccgaa tattttccgc ggtggggctg gccgggctcg gagtttcgcc atcaaaacgt
     3061 cggatcggct ggttgttcga acaagggctc gacgtttcga agaggctcgc ggaggcggtt
     3121 cggcactcga cctcgtatgc acgtcgacgc ttgcagcgcg ctcttcatcc catgtggcga
     3181 atattcgaag tcgcgaggac gcagcgtgac cgcggaggca acgcacttca atcagtgcgg
     3241 gctcgacgcg tttgagcgct cgtgtgcggc gctgagtccc catggagtga ggcaaggtga
     3301 cgaacgtcac gatgacgccg acggatggtc gtggggcgat cgtgctcgct cgcagcgctt
     3361 gaaggcgttc aatgggcgtg gtcttcttgc gccgagccgg agctttccgc gccgtctaca
     3421 aggagcaaga cgttggagtt tcatcgagga aggctaatcg accatgttca cttgcgggtc
     3481 gctgatgtcg aagcgagtaa gcgcttctat cgtgcggtgt tcgcgtcgct cgggctgccg
     3541 gacatcgtgc acgagggacc aggctttttt tacgcggacg aactcttcgt ggacaaggcg
     3601 gacgggtaca cgtcgcgcgt tcacctcgcg tttcaagccg cggaccgcgc aaccgtccaa
     3661 tcttttcatc gcgccgcgct cgcggcgggc ggtaaagaca acgggggccc cggcgagaga
     3721 tcgtatcacc ccgggtacta cggagccttc gtgctcgacc cggatggcaa caacatcgag
     3781 gcagtatttc acggtccgaa cgagcgcagc gcgccctcgg tcatcgtgac cgcgaagtag
     3841 cggcgggcat ggcttcgatc gtcgcagctc gtccgccttg cgactgagcg gggcgatgca
     3901 agactgagcg gg
//
LOCUS       DASTKS010000386         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1401145, whole genome shotgun sequence.
ACCESSION   DASTKS010000386 DASTKS010000000
VERSION     DASTKS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401145"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFQ35_08025"
     CDS             <1..515
                     /locus_tag="VFQ35_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930619.1"
                     /translation="FCPIWMGRAGLVSMLFLVSAVARATEPSAPVRTPAAATYAPVAL
                     PDQKPPPAAREEFDMPRLPAHPLRASLTSTELATVGAGEVTPRMIRVVLDTESVASFP
                     IDDARDDEPVSVPAMPRPRLLSIRRNLDDDGDVFERAGATHRLIRRSLDDASDLAPVS
                     AARRLRSSLD"
     gene            complement(522..2108)
                     /locus_tag="VFQ35_08030"
     CDS             complement(522..2108)
                     /locus_tag="VFQ35_08030"
                     /inference="COORDINATES: protein motif:HMM:NF041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6577-like cysteine-rich protein"
                     /protein_id="HET9930620.1"
                     /translation="MRLATVVFSVAFVLLASCSSRDVGSLFESGGSSGDLGAGGTTDN
                     GGMAGVGGVGGNLPACDVGIRCSGQCMDVSADPNHCGDCDTVCPLQAECRSGKCVCAN
                     GQAACGTRCVDVLSSTTNCGACNNACPPAAVCTAGECVCPDLQVACGQVCVDILTNAA
                     NCGACDKACPPRAACAAGLCVCPGSETACGAKCVDTQTSASNCGLCDNACPARANCVA
                     GECVCPEGETACAGKCVDTQTSTEHCGACGSTCRGGACTKGECVCPEGQVNCDGQCVD
                     LMTSAAHCGACETACANNHVCAAGACACATGLDECNETCVDLQKDKAHCGACATACPA
                     DKICMAGGCVCATGQTECDGKCVDLTKDAAHCGTCATACMAGMPCIKGICGCPPGQEL
                     CGTTCVDLTSDAAHCGSCVTACATSQFCTDKKCLKSPCDGLCANFETVPLGVDGFREE
                     NFNMSSHCYEVRGYEPTETNARIVCWNFGGRRLSVNGEVTECKADEGSPLTKKRADGY
                     CVQVGQGGGASSPGFLLPIK"
     gene            2399..3406
                     /locus_tag="VFQ35_08035"
     CDS             2399..3406
                     /locus_tag="VFQ35_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930621.1"
                     /translation="MVRRTLGGFVLLVSSLHAGAARAEMPCDGTRPFVRLELRGEAWT
                     TAQRERVVSDLSRSLAPQGIDVCSERSATLPAPLAELNIELSGPNKANVEILLQDSVT
                     HKRVARELDLAPIPADGRELAVAIEADELLRASWAELALDTERARRSKPRPEVVKSVD
                     QVLTPKRAKSALALEARFAAEHFADVDWFGGEAAARIPLPSRLSWELAAGVRLSPAQN
                     AEHGSVAALNAGLGTRLLVMIVGDDTASLHLGAGVIGSRVEFRGSASGNARADSYADW
                     LVVARGSVLGRLALGRLLGVSAGLSAGGALRGVEVTDSGRVVAAARGLELGATLGLEA
                     R"
     gene            3403..>4158
                     /locus_tag="VFQ35_08040"
     CDS             3403..>4158
                     /locus_tag="VFQ35_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930622.1"
                     /translation="MKSAPAALVALSVVACTGRGEVLREGNDTPVVLQQTEARLSTGD
                     EHACAVTSSALRCWGKSAEGELGLPLDGSHGPRQIDIGAPVRAPAAGALHTCALDTEG
                     RVFCFGSNDSGQLGTGAPLGSDDPAPGLVTLPAPAIDLRTLFSHSCAVLSDASLWCWG
                     ENEEGQLGLGDTHPGADHPAPIRVGGANDWVAVSTGQGHSCGIRAPGNLYCWGRNTQF
                     QIGQGSNDPQQYRAPQRVGTDTDWVEVSAGQNNS"
BASE COUNT          667 a         1423 c         1389 g          679 t
ORIGIN      
        1 cattctgtcc aatctggatg ggacgagcgg gtttggtttc gatgctgttc ctcgtgagcg
       61 cggtcgcgcg cgcgacggag ccgagcgcgc ccgtgcgcac gcccgcggct gcgacctacg
      121 cgccggtcgc cttgcccgac cagaaaccgc cgccggctgc gcgcgaagag ttcgacatgc
      181 cgcggttacc ggcgcacccc ttgcgcgcgt cgctcacgtc gacggagcta gcgacggtcg
      241 gggccggcga ggtcacgccg cgcatgattc gcgtcgtgct cgacaccgag tcagtcgcga
      301 gcttccccat cgacgacgcg cgcgacgacg aacccgtgag cgtgcccgcg atgccgcgcc
      361 ctcgcttgct ttccattcgc cgcaacttgg acgacgacgg tgacgtcttc gagcgcgcgg
      421 gcgcgaccca tcggttgatc cgccgctccc tcgacgacgc gagcgacctt gcgcccgtca
      481 gcgccgcccg caggcttcga agctcgctcg actgaaacgg ctcacttgat gggcagtagg
      541 aagccggggc tcgacgctcc accaccctgc ccgacttgca cgcaatatcc gtcggcccgt
      601 tttttggtga ggggcgagcc ttcgtcagcc ttgcactccg tcacctcccc gttgacgctc
      661 agcctacggc caccgaagtt ccagcacacg atgcgcgcgt tggtctcggt aggctcgtag
      721 ccgcggacct cgtagcagtg cgaagacatg ttgaagttct cttcgcgaaa accgtcgacc
      781 cccaatggta cggtctcgaa gttcgcgcac agtccgtcgc acggcgactt gaggcacttc
      841 ttgtcggtgc agaattggct cgtcgcgcaa gcagtcacgc agctgccgca gtgcgccgca
      901 tcgctagtca gatcgacgca cgtcgtgccg cagagctcct gcccgggcgg gcacccacag
      961 atgcccttga tgcacggcat gccggccatg caagcggtcg cgcaggtgcc gcaatgcgcg
     1021 gcatctttcg tgagatccac gcatttgccg tcgcactcgg tttgacccgt ggcgcagacg
     1081 cagccgccgg ccatgcaaat cttgtccgcc gggcaggcgg tcgcgcacgc accgcagtgc
     1141 gccttgtcct tctgcaaatc gacgcaggtt tcgttgcatt cgtcgagccc cgtcgcgcaa
     1201 gcgcacgcgc ccgccgcgca aacgtggttg ttcgcgcagg ccgtctcgca agcgccgcag
     1261 tgcgccgcgc tggtcatcaa gtcgacgcac tgaccatcgc agttcacctg cccctcgggg
     1321 cacacgcact cacccttcgt gcaggcgccg ccgcggcacg tcgaaccgca agcgccgcaa
     1381 tgctcggtgc tggtctgcgt gtcgacgcac ttgccggcac acgccgtttc gccctcgggg
     1441 cacacgcact ctccggcaac gcagttcgcg cgcgcgggac aggcgttgtc acacaagccg
     1501 caattgctcg cgctcgtctg cgtgtcgacg cactttgcgc cgcacgccgt ctcgctaccc
     1561 gggcagacgc acaggccagc ggcacaggcc gcgcgcggcg ggcaggcctt gtcgcaagca
     1621 ccgcagttgg ctgcgttcgt taggatgtcg acgcaaacct ggccgcaggc gacctgcaag
     1681 tccgggcaca cgcactcgcc cgccgtacac acggctgcgg gtggacaagc gttgttgcac
     1741 gctccgcagt tggtcgtgct cgagagcacg tccacgcatc gggtcccgca agcggcttgg
     1801 ccgttggcac agacgcactt gcccgaacga cactcggcct gcaacgggca caccgtgtcg
     1861 cagtcgccgc agtggttcgg gtcggcgctg acgtccatac actgcccgct gcagcgaatc
     1921 ccgacatcgc acgccggcag gttcccgccg actccgccga cgccagccat ccctccgttg
     1981 tcggtggtgc cgccggcgcc gaggtcgccc gagctccctc cgctttcgaa cagcgagccc
     2041 acgtcgcgac tactgcagct cgcgagcaga acgaaggcga ccgaaaatac gactgtggcc
     2101 agcctcatta agccaagcct cttttgcatg ggcgtctcca tcgccagcga cggacgtttg
     2161 cgggggaaga gcattcaccc ccggggtagc gtttcgtcaa ctcgtgtcac tgacttaggc
     2221 aatgctcctt caccatagaa ggaatgtcac aaccgtcttg aaaataaaga aagttgtcga
     2281 acgactgacg gaccgaacgg cggttgcgga cccgtgtggg gctgcggagt ctacacgctt
     2341 tgttgaacct tcggtacccc atcgcgggtc ctacacgcgc gtagggtatg gtcgaggcgt
     2401 ggttcggcgc accctgggag gcttcgtgct gctcgtaagc tcgctgcatg cgggagccgc
     2461 gcgcgccgag atgccgtgcg acgggacccg ccccttcgtg cggctcgagc tgcgcggcga
     2521 ggcctggacc acggctcaac gggagcgcgt ggtttcggac ctgtcgcgaa gcctcgcacc
     2581 tcaaggcatc gacgtgtgca gcgaacgctc ggctacgctg ccggctccgc tcgcggagct
     2641 gaacatcgag ctctccggac ccaacaaagc gaacgtagaa atcctgttgc aggacagcgt
     2701 gacgcacaag cgtgtggccc gtgagctcga cctcgcgccc atccccgctg acgggcgcga
     2761 gctcgccgtc gcgatcgagg cggacgagct tctccgcgcg agctgggcag agctcgcgct
     2821 cgacaccgag cgggcgcgcc gctcgaagcc gcgacccgaa gtcgtgaaga gtgtcgacca
     2881 ggtgctcact ccgaagcgcg caaagtcggc gctcgccctc gaagcgcgct tcgccgcaga
     2941 gcacttcgcg gacgtcgact ggttcggcgg cgaagctgca gcgcgtatcc ctctgccgtc
     3001 gcgcttgagc tgggagctcg ccgcgggtgt gcggctttct ccggctcaaa acgcggagca
     3061 cggcagcgtc gcggcgctga atgcagggct cgggacgcgg ctgctggtca tgatcgtcgg
     3121 cgacgacacg gcgtcgctgc acctcggggc gggagtgatc gggtcacgcg tcgagtttcg
     3181 gggatcggcc agcggaaacg ctcgcgcaga cagctacgcg gactggctcg tcgtcgcgcg
     3241 cggcagcgtg ctcggacggc tcgcgctcgg gcggctgctc ggcgtgagcg ctgggctcag
     3301 cgcgggcggc gcattgcgcg gcgtcgaggt gacagactcc gggcgcgtcg tcgccgccgc
     3361 gcgcggactc gagctcggag cgacgctcgg attggaggcg cgatgaagtc cgccccggca
     3421 gcgctcgttg cgctgagcgt ggtcgcttgc accgggcgcg gcgaggtcct gcgtgaaggc
     3481 aacgatacgc ccgtcgtcct tcaacaaacc gaggcgcggc tctcgacggg cgacgagcac
     3541 gcttgcgcgg taacgagcag tgcgcttcgc tgttggggca agtcggccga aggagagctc
     3601 gggcttccgc tcgacggtag tcacgggccg cgtcagatcg atatcggcgc gcccgtgcga
     3661 gcgccggctg cgggagcgtt gcacacgtgc gcgctcgaca ccgaagggcg cgtgttttgc
     3721 ttcgggagca acgacagcgg tcaactcggg accggcgctc cgctcgggag tgacgatccg
     3781 gcgccgggcc tcgtcacgtt gccggcgccc gcgatcgatc tgcgtacgct cttcagtcac
     3841 agctgcgcgg tgctgagcga cgcaagcctg tggtgttggg gcgagaacga ggaagggcag
     3901 ctcggactcg gggatactca ccccggcgct gatcaccccg caccgatccg cgtcggcggg
     3961 gcaaacgatt gggtcgcggt ctcgactgga caaggccata gctgcggtat ccgggctccc
     4021 ggcaatttgt actgctgggg acgcaacacg cagtttcaaa tcgggcaagg gagcaacgat
     4081 cctcaacagt atcgcgctcc ccaacgcgtc gggaccgaca cggactgggt ggaggtgagc
     4141 gcggggcaaa acaacagc
//
LOCUS       DASTKS010000387         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_730541, whole genome shotgun sequence.
ACCESSION   DASTKS010000387 DASTKS010000000
VERSION     DASTKS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730541"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFQ35_08045"
     CDS             <1..304
                     /locus_tag="VFQ35_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9930623.1"
                     /translation="FDTAVGERGQRLSGGQRQRISLARAILKDAPVLILDEATSAVDN
                     ETEAAIQRSLERVAVGRTTLIIAHRLTTIRNADQVFTLESGRIVDHRRRESANSEQ"
     gene            370..2631
                     /locus_tag="VFQ35_08050"
     CDS             370..2631
                     /locus_tag="VFQ35_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HET9930624.1"
                     /translation="MLFARHGSFTSISSLLVPLALASLATSCIKRENNCAPNGAPAQA
                     ANATAQLPNGPLPPGKSLLPEAALPAFVVQGEAASKVSVSTVAVEGQPFPDALHAEIK
                     ESSNSEWSVQLQAPTAAAVAKGDAILATFYVRLIKPNASGIGETQFVFEKAGAPYTKS
                     TTYNVRITPEWRKIEVRFQSAEDYGVAGAQMIFRMGYEPETFEVGGVKVENFGKVALS
                     ALPSSQGRDHQVEVATTVKEAPVTVVDGGELTFKVTPGKVVRQISPYVYGVNSQNLEG
                     LGTTVRRMGGNRGSVYNWETNASNAGNDYRHQNDEWSCSVMGYQTCSQPGAQYAEFVK
                     QNKAAGVESIVTVPMLDWVSADKNGPVAEAEKAPSKRFVHNYPKKKGALSTTPDLKDD
                     AVYEDEFVNLLVSKFGKAAQGGTKFYSLDNEPALWPATHPRVHASPTTYKEMVERTEA
                     TATSILGVDPSAFLLGGVMFGWSEYASLSDAPDAAEHNAKYGTYLDFFLASMKELEQR
                     HGKRLVHALDVHWYPEARGTKRITEKDNSRATVDARLQAPRSLWDPEYKERSWIIDKI
                     QKPIRFIPYLLETINKRYPGTKLTMTEYDYGGADHISGALAQIDVLGIFGREGMYMAN
                     FWGNGPGNGELEPYIASAFKLYRNFDGKGGHFGDTAVGATVADQKEGSIYAATDSKTP
                     GVLYVIVINKDQGKRFRAKIAIDGGAKYKKADVFVLDGSGPKVRPDKAVTVANNAIDY
                     QLGALSAALFVVQ"
     gene            2754..4097
                     /locus_tag="VFQ35_08055"
     CDS             2754..4097
                     /locus_tag="VFQ35_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9930625.1"
                     /translation="MARPPTSPRARLVKRLVLLAVLFSVGAGAFFGIRAYRARQQTGP
                     RFETVKVDRGRITARVTATGTLSALVTVQVGSQVSGRIAQINVDFNSEVKKGQVIAKI
                     DPELFRAALEQSRANFIAAKGNLAKAKAQAEDAARQAQRSTELAARELIAVADRDTAV
                     SNAAAAKAQVEASEGSMKQAEASLHSAQVNLAYTTIVSPIDGVVISRSVDVGQTVAAS
                     LQAPTLFTIAEDLKKMQVDTNVAEADVGKLQPGMAATFTVDAYPSERFRGKVRQIRNA
                     PQTVQNVVTYDAVIDVDNAELKLKPGMTANVTFIVSDKNDAVRLANAALRFRPPADMI
                     PGGASSAVAAGSAGPRGNWTPGASGAPRGAGRMGGGGNHERTLDTRTVWLLRGTTPES
                     HTVRVGVTDGSFTELTEGDVKPGDLLITGVTSDASGSPKTGLPGAGPGGPARRMF"
     gene            4105..>4623
                     /locus_tag="VFQ35_08060"
     CDS             4105..>4623
                     /locus_tag="VFQ35_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9930626.1"
                     /translation="MTEPPFRKRALIELEQVVKDYYLGEIELRVLKGITLSINDGEFV
                     AIMGSSGSGKSTLMNILGCLDRPTGGRYTLAGQDVSRLDRNELADVRNQTLGFVFQNF
                     NLLSRTSALENVELPLVYAGVKSHERHERALSALERVGLGSRTDHHPNQLSGGQQQRV
                     AIARALVGSPRLI"
BASE COUNT          909 a         1515 c         1466 g          733 t
ORIGIN      
        1 cttcgacacg gcagtcgggg agcgcggaca gcgcctttct ggcggacagc gtcagcgcat
       61 ctcgctcgcc cgagccatcc tgaaagacgc gccggtgctc attctcgacg aggcgacctc
      121 agccgtcgac aacgagaccg aggctgccat ccagcgctcg ctcgagcgcg tcgcggtcgg
      181 gcgcacgacg ttgatcatcg cgcaccggct caccaccatc cgcaacgccg accaggtatt
      241 caccctcgag agcggccgca tcgtcgacca tcgtcgccgc gagagcgcca attccgagca
      301 ataacggggg ttttctcacc cgctcaattt cgaagcaaaa ctccggaatt cgggtgtagc
      361 ttcccggcca tgcttttcgc ccggcacggt tcgttcacgt cgatttcgtc actgttggtt
      421 cccctcgcgc tcgcttcact ggccacttcg tgcatcaagc gagagaacaa ctgcgcgccc
      481 aacggcgcgc ccgcgcaggc cgcgaacgcg accgctcagc taccgaacgg accgttgcca
      541 ccgggaaaga gccttctgcc cgaggccgcg ctgcccgcct tcgtggtgca gggggaagca
      601 gcgagcaaag tcagcgtgag cacggtcgcg gtcgaaggac agcccttccc cgacgcgctc
      661 cacgccgaaa tcaaagagtc gtcgaactcc gagtggtccg tgcaactgca agcgccgacg
      721 gccgccgcgg tcgcgaaggg cgacgccatc ctcgcgacgt tctacgtgcg gctcatcaag
      781 cccaacgcga gcggcatcgg tgagacgcag ttcgtcttcg aaaaagccgg cgctccgtac
      841 accaagtcga cgacgtacaa cgtacgcatc acgcccgagt ggcgaaagat cgaggttcgg
      901 ttccaatccg cggaagacta cggcgtggcc ggcgcacaga tgatcttccg catgggttac
      961 gagccggaga ccttcgaggt cggcggggtc aaggtcgaga acttcggcaa ggtggcgctc
     1021 tccgcgctcc cgagcagcca gggccgagat catcaggtcg aggtggcaac gaccgtgaaa
     1081 gaggctcccg tcaccgtcgt ggatgggggg gagctcacct tcaaggtgac gcccggcaaa
     1141 gtggtgcggc agatcagccc ctacgtttac ggcgtgaatt cgcaaaacct cgagggcctc
     1201 gggacgaccg tacggcgcat gggcggtaac cgcggcagtg tttacaactg ggagaccaac
     1261 gcctcgaacg cgggcaacga ctaccgccac caaaatgacg agtggtcgtg ctcggtgatg
     1321 ggctaccaaa cgtgttcgca gccgggcgcg cagtacgccg agttcgtgaa gcagaacaag
     1381 gcggcggggg tcgaatcgat cgtcaccgta ccgatgctcg actgggtgag cgccgacaag
     1441 aacggccccg tcgcggaagc ggagaaggcg ccgagcaagc gcttcgtcca caactatccg
     1501 aagaagaagg gcgcgctttc gacgactccg gacctgaagg acgacgccgt ctacgaagac
     1561 gagttcgtga accttctggt cagcaagttc ggcaaagccg cgcaaggcgg aacgaagttt
     1621 tactcgctcg acaacgagcc cgcgctgtgg ccggcgacgc acccgcgcgt gcacgcttcg
     1681 cccaccacgt acaaggagat ggtcgagcgc accgaggcca ccgccacgtc catcctcggg
     1741 gtggatccga gcgcgttctt gctcggcggc gtgatgttcg gttggagcga atacgcttcg
     1801 ctgtccgatg cgccggacgc ggccgagcac aacgccaagt acggcaccta cctcgacttc
     1861 ttcttggctt cgatgaagga gctcgagcaa aggcacggta agcgcctcgt gcacgccctc
     1921 gacgtgcact ggtaccccga agcgcgcggc acgaagcgca tcaccgagaa ggacaactcg
     1981 cgcgcgacgg tggacgcacg cctgcaagcg ccgcgcagcc tgtgggatcc ggagtacaaa
     2041 gagcgcagct ggatcatcga caagatccag aagccgatcc ggttcattcc gtatctgctc
     2101 gagacgatca acaagcgcta cccgggcacg aagctcacca tgaccgagta cgactacggc
     2161 ggcgcggatc acatttccgg cgcgctcgcc caaatcgacg tcctcggtat cttcgggcgc
     2221 gaaggcatgt acatggcgaa cttctggggc aacggtccgg gcaatggtga gctcgagccg
     2281 tacattgctt ctgcgttcaa gctttaccga aacttcgacg gtaagggcgg ccacttcggc
     2341 gacaccgcgg tcggcgcgac cgtcgccgat cagaaggaag gctcaatcta cgccgcgacc
     2401 gactcgaaga cgcccggtgt gctctacgtc atcgtgatca acaaagatca gggcaaacgc
     2461 ttccgcgcca agatcgccat cgatggcggc gcgaagtaca agaaggcgga cgtcttcgtg
     2521 ctcgacggca gcggcccgaa ggttcggccg gacaaggctg tcacagtcgc caacaacgcg
     2581 atcgactacc agctgggcgc cttgagcgcg gccttgttcg tcgtgcagtg accggcccgt
     2641 acggctccgc cagacattgc ccggaattat tgcgatattt taagggctga aacaggcgag
     2701 cgcgcggcag cgtagggtgg gggcactttc gatcgttcaa ccgacgtact caagtggccc
     2761 gacccccaac ttctccgcgc gcccgcctcg tcaaacgcct cgttctattg gctgtgctct
     2821 tcagcgtggg cgcgggtgcc tttttcggca ttcgcgccta tcgcgcacgc cagcagacgg
     2881 gccctcgctt cgagacggtc aaagtcgacc gagggcgcat caccgcccgc gtcaccgcga
     2941 ccggtacgct gtcggcgctc gtcaccgtgc aggtcggcag ccaggtctcc gggcgcatcg
     3001 cgcagatcaa cgtcgatttc aactcggagg tgaagaaggg gcaggtcatc gccaagatcg
     3061 atcccgagct cttccgcgcc gctctcgagc agtctcgcgc aaacttcatc gcggcgaagg
     3121 gcaacctcgc caaagcgaag gcgcaggccg aagacgccgc acgccaggcc cagcgcagca
     3181 ccgagctcgc cgcgcgtgag ctcattgcgg ttgcggatcg cgataccgcc gtctccaacg
     3241 ccgccgccgc caaggcccaa gtcgaagcga gcgaaggcag tatgaagcaa gccgaggcgt
     3301 cactccactc ggcgcaagtg aacctggcgt acacgaccat cgtttccccc atcgacggcg
     3361 tggtgatctc gcgtagcgtg gacgtcgggc aaacggtggc cgcctcgctg caggcgccga
     3421 cgctgttcac gattgcagag gacctgaaga agatgcaggt cgacacgaac gtcgccgagg
     3481 cggacgtcgg gaagctgcaa ccagggatgg cggcgacgtt cacggtggac gcttaccctt
     3541 cggagcgttt tcgcggcaag gttcggcaaa ttcgcaacgc gccgcaaacg gtccagaacg
     3601 tcgtcaccta cgacgccgtg atcgacgtcg acaacgcaga gctcaagctg aagccgggca
     3661 tgacggcgaa cgtcacgttc atcgtctccg acaagaacga cgccgttcga ctcgcaaacg
     3721 ccgccctgcg ctttcgccct cccgccgaca tgattccggg tggagcttcg agcgccgtcg
     3781 ccgccggctc ggcaggcccg cgaggtaatt ggacgcccgg cgcttcaggc gctccgcgag
     3841 gtgccggacg catgggcgga ggcggcaatc acgagcgcac gctcgatact cgaacggtgt
     3901 ggttgctgcg cggcacgact cccgaatcgc atacggtgcg agtgggcgtg accgacggaa
     3961 gtttcaccga gctcaccgag ggcgacgtaa agccggggga cctgctcatc acgggcgtca
     4021 ccagcgacgc gagcggctca cccaagacgg gcttgcccgg cgcgggaccc ggcggtcctg
     4081 cccgccgaat gttctgaggc ttcgatgacc gagccgccat tccgcaagcg tgccctcatc
     4141 gagctcgagc aggtggtgaa agactactac ctcggggaaa tcgagcttcg cgtcttgaag
     4201 ggcatcacgc tctccatcaa cgacggagag ttcgtcgcga ttatggggtc ttccggttcg
     4261 gggaagtcca cgttgatgaa catcctcggc tgcttggatc ggccgacggg aggacgttac
     4321 acgctcgccg gccaggacgt gtcgcggctc gaccgcaacg agctcgccga cgtgagaaac
     4381 cagacgctgg gcttcgtttt ccagaatttc aacttgctct cccgcacgag cgcgctcgaa
     4441 aacgtcgaat tgcctctggt ctacgccggc gtgaagagtc acgaacgtca cgagcgcgcg
     4501 ctttcggcgc tcgaacgtgt cgggctcggc tcgcgcacgg atcaccaccc gaaccagctc
     4561 tcgggcgggc agcagcagcg ggtcgcgatc gcgcgggcgc tcgtcggctc gccgcggctc
     4621 atc
//
LOCUS       DASTKS010000388         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_954062, whole genome shotgun sequence.
ACCESSION   DASTKS010000388 DASTKS010000000
VERSION     DASTKS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954062"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..>3510
                     /locus_tag="VFQ35_08065"
     CDS             271..>3510
                     /locus_tag="VFQ35_08065"
                     /inference="COORDINATES: protein motif:HMM:NF045168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930627.1"
                     /translation="MPTVVTSKHLQGTTELTLIAEIKPGLVPVPEPMSYAFRLRRVLD
                     FLFQARQAAVERFGQVGNAGPLERLRMLHFVHWSIHDADRKLLLAVSFDGPWEPYIHS
                     IVEEAGPILDVIFSHCDGYAGKSCFDGYEAFSAWVRERQINCDFFYAGAPDVTVDDVR
                     YLQEFERHHANGKRRAPFEAVSSRLRVGEPPAPQLDAAARKRLLGGLFALRALFPEGG
                     AGSVGAANDAFVFGRFAALLTRDVRAALVNGAGAAPSWFPSPPRKPSSAELELLQWAR
                     EELRRFDATDKPEVPAEEPRNPHEIQGNVLTGYEDVTHGCALLGRFESPAGALKFIER
                     WKEALTTEEGRGSPLGLGLSAGTRMNLALTSNGLASLGVASEELALFPKEFQEGLAAR
                     AGMLGDIGRNHPARWQLPEHNWPKPQGDRIPLAAVDFVIVLQRSAPQEPLDYRLTDRH
                     PLFEDVKTIAESGGAELLHVRPLLHYERGHFGIADGKSQPRHRATSASVPARDKVALG
                     ELLLGYQNDRGEVRLGSLPRELVENSTFLVMRQMSLDVPGFREAVSEASNSGIDQETY
                     LAKVVGRRADGTPLLEGGPSENDYDYQSDPHGAKCPLFSHARRANPRMSRRIEGRVVS
                     SPRIVRRGLSYGPLAVEEELPGSPERGLLFMSYCASIADQYEVIQRWVNGGNSSGVLS
                     SHADPLAGNFPERNGFERLLSCVHEGQPISIPLPARPLSELKWGLYLYVPSIGALDFL
                     LSRAGKKVAPGEPSSRAVRDFLTEMQELESLQPHRAALAWKLVLEDRDQRVRAAAIWE
                     FVRRNGGALRTAYGVLVGSAEGVLTVLSEHKRFSVRRYWHRMQKSFGVHYLGMDPEPQ
                     PLERACPVSAPRDGGDFAEEGDEYPLGAGPYVPDGAYHRVSELPNEFALSIGSAEAYR
                     SAYSLTRRWLESREDGLVDLRQLSREVIFEVARELIGTPEPPAMDASAERPGGDGPAR
                     CPVDLQRVSQFIFHPRPSVALARAAELRGKVVLDTMTAFVSDPAPTARFSKFFRERIG
                     KIETELTQFTGSDEEGFELDGMRGEASALTGLINGFCVPTSGTFLSVMSQ"
BASE COUNT          565 a         1153 c         1167 g          625 t
ORIGIN      
        1 ttccagcgcg taattctcca tgttttcgtc agagtctcct cggctcggcg caggtcgtgc
       61 tcagagcccg gcgcagcatc gccggaatgg cgagcgccgg gtatgaagcg attcacaccc
      121 gacgcgaaac ggttccggcc gatcatcgaa acttttacct acgcttcgtg ttagcgatct
      181 cacatcctac cctcgttcgc ggcattagga ttcgagcccg ctcgcgcgcc acttagcgcc
      241 gcctcccaag gatccaccga ggagcttttc atgccgacgg tcgtgaccag caagcacctg
      301 caaggaacca ccgagcttac gctcatcgct gagatcaagc ccggactggt cccggtgccc
      361 gaaccgatgt cttacgcgtt ccggctcagg cgcgtgctcg attttctgtt ccaggcccgg
      421 caagccgcgg tcgagcgctt cgggcaagtg gggaacgcgg ggccactcga gcgcttgcga
      481 atgctccatt tcgttcactg gtccattcac gacgccgacc gcaagctcct cttggcggtg
      541 agcttcgatg gaccttggga gccgtacatc cactcgatcg tcgaggaggc ggggccgatt
      601 ctcgacgtga ttttctctca ctgcgacgga tatgcgggca agagttgctt cgacgggtac
      661 gaagcatttt cagcgtgggt gcgcgagcgc caaatcaact gcgacttctt ctatgccggt
      721 gcgcccgacg tcacggtcga cgacgtgcgc tacttgcagg agttcgagcg ccaccacgcg
      781 aacggcaaac gccgcgcgcc gttcgaggcg gtctcgtcca gactgcgcgt cggcgagcca
      841 cccgctccgc agctcgacgc cgccgcgcgt aagcgcctgc tcggcggtct tttcgctctg
      901 cgcgcgcttt ttccggaagg cggcgcgggc agtgtgggcg cggcgaacga cgctttcgtc
      961 tttgggcgct tcgcggcgtt gctcacgcga gacgtccgcg ccgcgctcgt gaacggcgcc
     1021 ggtgcggctc cgagctggtt cccgagtcct ccccgcaagc cgagctcggc tgagctcgag
     1081 ctgctgcagt gggcgcgcga agagctgcgc cgcttcgacg cgaccgacaa gccggaggtg
     1141 cccgccgagg agccgcgcaa cccgcacgag atccagggga atgtcctcac cggttacgaa
     1201 gacgtgacgc acggctgcgc gctgctcggg cgcttcgagt caccggcggg cgcgctgaag
     1261 ttcatcgaac gctggaaaga ggcgctcacg accgaggagg ggcgtgggtc gcctcttggc
     1321 ctcgggctct ctgccggcac ccgcatgaac ctcgccttga cctcgaacgg gctcgcgtcg
     1381 ctcggggttg cgagcgaaga gctcgcgctg ttccccaaag agttccagga agggctcgcg
     1441 gcccgcgcgg gcatgctcgg cgacatcggc cgaaatcatc cggcgcgctg gcaactcccc
     1501 gaacacaatt ggcccaagcc gcagggagac cgcatcccgc tcgcggcggt cgacttcgtc
     1561 atcgttttgc agcgaagcgc gccgcaggag ccgctcgact accggttgac ggataggcac
     1621 ccgctgttcg aagacgtgaa gaccatcgcc gaatcggggg gcgccgagct ccttcatgtt
     1681 cggcccttat tgcactacga gcgcgggcat ttcggaatcg ccgacggcaa gagccagccg
     1741 cgccatcgcg cgacgagcgc gtcggttccc gcgcgcgaca aagtcgcgct cggggagctc
     1801 ttgctcggct accaaaacga tcgcggtgag gtgcgcctcg ggagccttcc gcgtgagctc
     1861 gtcgagaact cgaccttcct cgtcatgcgg cagatgtcgc tcgatgttcc ggggtttcgc
     1921 gaagcagtct cggaagcgag caactcgggc atcgatcagg agacttatct tgcgaaagtg
     1981 gttgggcgtc gcgccgacgg gacgccgctc ctcgaaggcg gtccgagcga gaacgactac
     2041 gattaccaga gcgatccgca cggcgcgaag tgcccgctct tctcgcacgc gcggcgcgcc
     2101 aacccgcgca tgagccggcg tatcgagggg cgagtggttt cgtcgccgcg catcgtgcgc
     2161 cgcggcctga gctacggacc gctcgcggtc gaggaggagc tgcccggtag ccccgagcgc
     2221 ggcctcctct tcatgtctta ctgtgccagt attgccgacc agtacgaggt aattcagcgc
     2281 tgggtgaacg gcggcaattc gagcggcgtg ctgagctccc acgccgatcc gctggcgggc
     2341 aattttccag agcgcaacgg cttcgaacgc ttgctcagct gcgttcatga agggcagccg
     2401 atcagcattc cgcttccggc gcgaccgctg tcagagctca agtggggact ctatctctac
     2461 gtgccgtcca tcggcgcgct ggatttcctg ctgtcgcgcg cggggaagaa ggtcgcgccg
     2521 ggggagccga gctcgcgcgc cgtgcgcgac ttcttgaccg agatgcagga gctcgagagc
     2581 ttgcagcctc accgcgctgc gctcgcctgg aagctcgtcc tcgaagaccg cgaccagcgc
     2641 gtgcgggcgg cggcgatctg ggagttcgtc cgtcgaaacg gcggcgcgct tcgcaccgcg
     2701 tacggcgttc tcgttggcag cgccgaggga gtgctcacgg tgttgagtga gcacaagcgc
     2761 ttttcggtgc gtcgctactg gcatcgcatg cagaagagct tcggggtgca ctacctcggc
     2821 atggatccgg agccgcagcc gctcgagcgc gcctgcccgg tgagcgcgcc gcgcgacggc
     2881 ggcgacttcg ccgaagaggg agacgagtat ccgctcggcg cggggccgta cgtccccgat
     2941 ggggcgtatc accgcgtttc ggagctgccg aacgagttcg cgctctcgat tgggagcgcc
     3001 gaggcctatc gttcggctta ctcgctgacg cgtcgctggc tcgagagccg tgaggacggg
     3061 ctcgtcgatc tgcgtcagct ctcgcgcgag gtgatcttcg aagtcgcacg cgagttgatc
     3121 ggcactcccg agccgccggc aatggatgcg agcgccgagc ggccgggagg cgatggacct
     3181 gcgcgctgcc cggtcgacct gcagcgtgtg tcacagttca ttttccatcc acgcccctcg
     3241 gttgcgctgg cacgcgcggc agaactgcga ggcaaggtcg tgctcgacac catgaccgcg
     3301 ttcgtcagcg accctgcgcc gaccgcccgc ttctccaagt tcttccgaga gcgtatcggc
     3361 aagatcgaga cggagctcac gcagttcacc ggttcggacg aagaaggctt cgagctcgac
     3421 ggcatgcgcg gagaggcttc ggccttgacg gggctcatca acggcttctg cgtgcccacg
     3481 agcggtactt tcctgagtgt catgagccaa
//
LOCUS       DASTKS010000389         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_509243, whole genome shotgun sequence.
ACCESSION   DASTKS010000389 DASTKS010000000
VERSION     DASTKS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509243"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFQ35_08070"
     CDS             complement(<1..559)
                     /locus_tag="VFQ35_08070"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9930628.1"
                     /translation="MTGRSDSALPWSWRLLRAAVIAAERVSPQTVAHTVAWLMFRTRR
                     SAPPPREADWLASGERWELAGPLGRLVAFRFGRGPVVVLVHGWNGRGGQLGAFIAPLV
                     RAGFSVVTFDAPGHGFSQGAEASLVAFADAFDRVVSALRENGEDVRGVVAHSLGAAAV
                     TFSESRRVRGQSTAALRRLVFVAPPV"
     gene            complement(602..1192)
                     /locus_tag="VFQ35_08075"
     CDS             complement(602..1192)
                     /locus_tag="VFQ35_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9930629.1"
                     /translation="MSKGEATRERIVDRAWRLASRDGLAGLSLQRLATDLGLSKSGLF
                     AHFGSKQELELEVLKCASEHFIDKVIRPALKAPRGVPRLRQLFKNWISWANDPAVPGG
                     CVFLAVATELDDSEGPQRDYLVTTQSALLATLAKAARLAIEEGHFRGNVDCEQFAFDL
                     LGVVLAYHHARRLLRLPKAEARAQKAFDALLQAAQA"
     gene            complement(1262..2269)
                     /locus_tag="VFQ35_08080"
     CDS             complement(1262..2269)
                     /locus_tag="VFQ35_08080"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HET9930630.1"
                     /translation="MNYKKPSDSELRQRLTPLQYEVTQHDATEPPFRNAFWDNHEDGL
                     YVDVASGEPLFSSRDKFDSGTGWPSFTRPLEGERIVEKTDRAHGMVRTEVRSRDANSH
                     LGHVFPDGPAPSRLRYCINSASLRFIPVARLEAEGYGKYLSSFEGMPGATLTDTNNVC
                     ARPAPGEGPSCTATLETVVLAGGCFWGMEDILRQIPGVLETEVGYAGGTTKNPTYNDV
                     RTGATGHAEAVRVTFDPSKLSFESLLENWFFKMHDPTTKNRQGNDVGTQYRSAILVTS
                     PAQRATAEAVRERVDKSGKWRKPIVTEIVEAGSFTRAEEYHQKYLVKNPGGYTCHWMR
                     D"
BASE COUNT          455 a          840 c          794 g          440 t
ORIGIN      
        1 cgaccggggg tgcaacgaac accagtcggc gaagcgcggc cgtcgactgg cccctcactc
       61 gccggctctc ggagaacgtc accgcggccg cgccgagcga atgcgcgacg acgccgcgca
      121 cgtcttcacc gttttcgcgc agagcgctca cgacgcgatc gaaggcgtcc gcgaacgcaa
      181 cgagcgaggc ttcggcccct tgcgaaaatc cgtgacctgg cgcatcgaac gtcaccacgg
      241 aaaacccagc cctaacgagc ggagcgatga acgccccgag ctgaccaccg cgcccgttcc
      301 aaccatgcac caagacgacg accggaccac gcccgaagcg aaacgcgacc aaacgcccca
      361 ggggccccgc gagctcccaa cgttcgccgc tcgccaacca gtcggcttcc cgaggtggag
      421 gcgcgctacg tcgcgtccgg aacatgagcc aggccaccgt atgcgccacc gtttggggcg
      481 agacgcgctc ggccgcgatc acggcggcgc gcaaaaggcg ccaagaccag gggagcgcag
      541 aatcagaacg accggtcatg cttttttctg ctgaaatgga gctcaccaca tcctccaaca
      601 atcacgcttg cgctgcttgc aggagcgcgt cgaatgcctt ttgtgcacgc gcctctgcct
      661 tggggagccg aagcaatctg cgcgcgtgat ggtacgcgag cacgactccg agcaggtcga
      721 acgcaaactg ctcgcagtcg acgttgcccc gaaaatgtcc ctcctcgatc gcgagccgcg
      781 ccgctttcgc gagcgtcgcg aggagcgcag actgcgtggt caccaagtag tcgcgctggg
      841 ggccctctga atcgtctagc tcggtcgcaa ccgccaggaa aacacagccg cccggcactg
      901 ctgggtcgtt ggcccacgat atccagttct tgaacagctg ccgcagccgc gggaccccgc
      961 gcggcgcctt caaagcgggt cggatcacct tgtcgatgaa gtgctctgac gcgcacttca
     1021 gcacctcgag ctcgagctcc tgcttcgagc cgaagtgggc gaacagtccg ctcttcgaga
     1081 gcccgagatc ggttgcgagg cgctgcaagc tcaagccggc gagcccatcg cggctcgcca
     1141 gccgccaagc gcgatcgacg atgcgttcgc gggtcgcctc acctttgctc acgccgcttg
     1201 attacgaccg gtcgtgctat ttgtcaactt cggaccgttc gcggcccagc cgtgagagca
     1261 ctcaatcgcg catccagtgg cacgtgtatc cccccggatt cttcaccaaa tacttctggt
     1321 gatactcctc cgcgcgcgtg aacgacccgg cctcgacgat ttccgtgacg atgggctttc
     1381 gccacttgcc gctcttgtcg acgcgctctc tcaccgcctc ggcggtggcg cgctgagccg
     1441 gcgaagtcac caagatagcc gagcgatact gggtgccgac gtcgtttcct tggcgattct
     1501 tcgtcgtcgg atcgtgcatt ttgaagaacc agttttcgag cagcgactcg aagctcaact
     1561 tactcggatc gaacgtcacg cgaacggctt cggcgtggcc cgtcgcgccc gtgcgcacgt
     1621 cgttataggt cgggttcttc gtggtgccac cggcgtaccc aacctcggtc tcgagcacgc
     1681 ccgggatttg ccggaggatg tcctccattc cccagaagca cccgcccgcc aggacgactg
     1741 tttctagagt cgccgtgcag ctaggtccct cacccggcgc cggacgcgca cacacgttgt
     1801 tcgtatcggt gagcgtcgcg cccggcatgc cctcgaacga actcaggtac ttgccgtaac
     1861 cctcggcctc gaggcgggcg accgggatga agcgtagcga cgcggagttg atgcagtagc
     1921 gcaggcggct cggcgcggga ccgtcgggga acacgtggcc aaggtgcgaa ttggcgtcgc
     1981 gcgagcgcac ttcggttcgg accattccgt gtgcgcgatc cgtcttttcg acgatgcgct
     2041 cgccttcgag cgggcgcgtg aaactgggcc aaccagtccc cgaatcgaat ttgtcgcgag
     2101 agctgaagag cggctcgcca ctggcgacgt ccacgtagag gccgtcttcg tggttgtccc
     2161 agaacgcatt gcggaacgga ggctcggtcg cgtcgtgctg ggtgacttcg tactggagcg
     2221 gcgtcaggcg ttgccgtagc tcgctgtcgg aaggcttctt gtagttcatc gtccgtctct
     2281 tctcggtagc cgtgcgtaga accgctgaga gcgttgctta gcacgaagag gcgaacccat
     2341 cttccgagca ccatcacgac ggtgcccgcg agcgagacgc gcggtggggc gaaattggac
     2401 ggcgagacaa gggggggcgc gagccgaggc ttggagccgt tacaaacgtg aaaacgccgg
     2461 gacctttggg agatcccggc gtcttggatg aacgctcgcc gcgcgctact ggtgcacgtc
     2521 agtgtcgat
//
LOCUS       DASTKS010000390        12697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_63311,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000390 DASTKS010000000
VERSION     DASTKS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63311"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1676)
                     /locus_tag="VFQ35_08085"
     CDS             complement(228..1676)
                     /locus_tag="VFQ35_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9930631.1"
                     /translation="MRPGLRLQLLVLLGGLLLLSFVPLYAAVSTYTKVALQQIRIGHA
                     RALGRAVAAQVSEAHTRRSPGELDGLLRAEVGTAGVETISIFSREGIVLAHAGAKASP
                     ELAQRLDVRREATFELEGAHGRALAVVVPSGDAAVAAVLRIDDDAAHAAPLLRLLGLY
                     TTLVALSLLGLSYFALTYLIVRPLDELGRAAERVARGARRLKLPLLRSRELDELGSSV
                     ASMTERLISEEEALRKKIDEVEAATRRLTEAQDRLVRSERLASVGRLAAGLAHEIGNP
                     IAALIGLEDLLLAGGLEPAEERDFLTRMRRETERIHRILRDLLQFARPAAGAADERPA
                     PGDVARAVHDTVELVSPQKDLKGVEIRVELADSLPPVSLGSEQLVQVVLNLLLNAADA
                     VGAGGHIVVAAREVEGTVSLTVTDDGPGVQNEMRPRLFEPFATTKDPGKGTGLGLAVC
                     RGLVEAAGGSISLDEAHSPGARFVVELPIVTA"
     gene            1891..4029
                     /locus_tag="VFQ35_08090"
     CDS             1891..4029
                     /locus_tag="VFQ35_08090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9930632.1"
                     /translation="MDPLVRSTLWVQRQLSRGAGTALATLFGGYLVLCTLDPRPRAEA
                     LVVLGAFVASTAYRAYQRLRRPPAEDESTRLDLELFTHLAVAAYAAVLRAPGGLDGPM
                     YPVLYALAMLLASFARPPAAAGSLAFALLLESALHYIALAERGAERLIAHGVLLGAFT
                     ILNFVVFRAEIARVRSLSRLRIETELTKMREAARTYRLLGAPSAASERSQTPPPDDPE
                     RLLRSGVDEIHQAVAFALGLLRRTLGLRTAMLLGLDANGTSLSIQELDTSEDAILPGP
                     FGARDGILGASLAKCEQLSVAGPKAGLHVPYYAVRPSIGSASATPLLEHGQPRGLLIV
                     DRESREPFTRDEEETLQRAARFLLRAIENERVFVQLERAKVEQGKLYRAANALASATT
                     EASVIEASVNGAREFASFDFAVVTLFDKATNEHEICAVSGQGASELVGQRFRHNQGLV
                     SMVVANRHALPYRGDYDPSRQMVFTRRLAPPSMPSLLVLPLLVHDRALGTLVLGSQRR
                     GAFGDAVRPTLEVLASHVAVSLSNARMLKRLEELATLDGLTGLFNKRALTELSLHKLR
                     SATRFKKALTLLICDIDHFKKVNDTYGHDVGDQVIKGFAEVLKRVKRDTDAVGRFGGE
                     EFVVLCEETDERGAAQLAERIRTELEATTFHSELGQLKVTCSIGVAPFPAAGATWEPL
                     FKAADEALYASKRGGRNRVTIWSSKLGNVA"
     gene            complement(4036..4854)
                     /locus_tag="VFQ35_08095"
     CDS             complement(4036..4854)
                     /locus_tag="VFQ35_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930633.1"
                     /translation="MVSNDFGIYASGGYSSRGSRFSRAVYLFSWSLVGVAGVAGLLFS
                     LYRNDVLFQEARRRGMERPYLALERRLLGTPGWGTPRSLLAPEALADSTAAALGAPVA
                     FGGTASPAATPAAAPSPPPSVASEPTASRTAEPKATPEPKATTESKPTPEPKPAAPTS
                     GGVTSEGVKITSLDALAPLPRGASHAAAPVTHTAAPVARAAAPTRPAHVAESKPSPAR
                     FAAASEPKAKPAPPPAKAAVPKPAPAPKAAPPPRDDNPLNAAIRGVIAKDAAGK"
     gene            complement(4929..5801)
                     /gene="epsC"
                     /locus_tag="VFQ35_08100"
     CDS             complement(4929..5801)
                     /gene="epsC"
                     /locus_tag="VFQ35_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET9930634.1"
                     /translation="MALRTRELEAAIDLIAKSYEGRDEINNLATAALPNKSAVVEAYL
                     HIRPVIYAGFYSTRELDSGSLRFTLSEHLYPAYESLVEQIERVLAYEHRFHGVPLEAG
                     LAEEVVLTLFRKLPEVRATLNQDLVAAFEGDPAAKSIEEIVFSYPAVEAITAHRIAHE
                     LFTASIPIIPRIISEYAHSRTGVDIHPGARIGPRFFLDHGTGVVVGETSVIGSNVKIY
                     QGVTLGALSTRRAECSRNHKRHPTIEDNVTIYAGATILGGETVIGAGSVIGGNVWLTQ
                     SVPPGSKIFGRARE"
     gene            complement(5852..7492)
                     /locus_tag="VFQ35_08105"
     CDS             complement(5852..7492)
                     /locus_tag="VFQ35_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET9930635.1"
                     /translation="MKRLSNGSISAWALSTVLASWAQVAAAQPAPRPAQPAPAPIRPA
                     PPAAGAPAPVAPGPAPAPAPGPVAQPAPAAPVPAPAVPPAVVQPAPATPPPVAPTDPA
                     PSAPSSSPETPAATVIPPANEPPPAAPPPSVPSVAEPPPAAPPPPVAESAAWYEGLAF
                     SAFVDAYGAVDFNFPKSPGQSRSNPVRAFDNHNGFALNWVGLDVQKDPAPVGGTLSLR
                     FGPGAQRLASACVDADTCDSESSLGLSYVKQAFVSWKPLDALTLDLGKFDTPYGAEVA
                     DSQYNINYTRGALYWLGQPAYHTGLRVGIDAARSFNLKLLAVNGWNRTIDNNSGKTFG
                     LQGTYRLPKAEGSDEDLLTVAVGYLVGPEHGDTQTLVCPAGQRFDPTRGCVLSQASEG
                     GSGVADLGSSNTKGLRHFLDLTAVATPIPELKLLLNASLGIDRARDPGDITKFRSATW
                     WGVMAGARYAVNEHFGVAGRIEHYADPDGYTTGFAEDVVVRTGTLTLDYIPAEHMVVF
                     LDGRADWSSKQIFPKAVHEEERAASAVSVTLGAVVSTN"
     gene            complement(7621..8661)
                     /locus_tag="VFQ35_08110"
     CDS             complement(7621..8661)
                     /locus_tag="VFQ35_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930636.1"
                     /translation="MARFRVEFFALAVALAHAACSEQERPGAPQANSSGGRSSGGFAN
                     TSGGASRGGSLGAGGSAGHAGVGEAGEAGAAGDGGARGEPLPPLCSPEQNWSEGQRLA
                     FSAAGDDELVGVSGDELSIAWFAAGELRYADRAKRTDAFGAPVPVAGGADYFGGATLS
                     SDGLSLIAVRKDGKSFGLLRRASRTDAFAGAFDEAPFVQIAAAPGTFRARGPFGDPVL
                     APNGLSLVYSNLDPAADGGPSLYESSRLGSRDAWPFGEPIDGNMLVVSGSSFRRPSAI
                     STDKRTLFYWDEAERESRAAFRSYASGPFETAVSLGERARAVPNAECDRLYYSAPGAQ
                     GLDVFVASLTPP"
     gene            complement(8670..12551)
                     /locus_tag="VFQ35_08115"
     CDS             complement(8670..12551)
                     /locus_tag="VFQ35_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930637.1"
                     /translation="MGTRTCSNGVWSACSVTRHVTKFAPQSGDKHLLALATTPESCHE
                     PCDPNCSVFIDTPPGIDPGNDFTLKGGTLTLPARAGGGSCAQLRLSPSDAVITVTSLT
                     PLVTDPPTIQFSATCGLGDEPIQPSWVLRASDADVARIDGNGVLRALAALPKTVQVTA
                     ATAAGQASATVDVRVNIDEVDADCDATLFSGSTSGVDPGKTLYPYSVPARPVVFPLGL
                     PGPLVQWSTGGVTADCVKVSLEFPRGAPAPKFHWSRVYSSAASATDPKVVDSTQPAAP
                     IPDDVWKAFSSAAAGQVTDIQVQRYPEGAPAPLEPLPAIPLKFANDSIRGTAFFTQYL
                     RRLRDDQNLPSVNICGDPDYGQQPDLDIRDTRFRTDSASGCVNGDCSANSAPSCPVGG
                     CTQAVTFRSQVATLQALDLSNPSLGLTDPFGASSANRCTACHSISADGRTLVAGDYSS
                     SGLQTIARLDTIGGRAHANPIADAPTYSWTSSRGDSIQPEREQSKGLSYAALTPDGRY
                     VLQGANFWGNTDHDVLLGSENLQDAGYPAGGRRYFLLDVRHYRKNVQFATNGPLPSHT
                     FASGTLTGTANGPLTIDGNGVAVGDSVLVKDQPTASENGVYTVAVSRLSSPFRLVRRV
                     EDGAVPLGFGDKFRVEHGDTNAGKYFHLASPMGAITPDVTPLTFALYDTVMTSTSRAL
                     PAHNYLLGGDGVPILTGSGTFDSDWIDDVPPENSMSVLVKNEVGANAIENGIYELVDA
                     NSNPWVLRRRSEADSDPELVGGARVRVNQGTRFGGMTFVLEGTGVQRLGVASLLFPRD
                     TSSLDEGMKYDRGGTPSTLPTMMFPSFSPDGQSLVYVNGDSDTLGTTALATGWRRGLS
                     LLSFNANAAVPFSGKRRLLNSYDSSVPGTVFKWPFFEHDSRSVIFQASAADEFCPLEA
                     YQGHCTGPDCAASTEGIAVDSDLERACFKWSDWKEYGHGNGAPTARGYWPGRLESVNT
                     TTLVRTALAHANAGLADQDATRLAADAAKSYQPNVVPFTAGGYRWVVFTSTRAYGNQL
                     NPVGTHFTCAMPLLWMAALDDTGAGSDDRSYPAFLLPGQNLRSIFAETTQPPFGRHYL
                     NERAYVVPSACKGEHAPCTADGDCCDSTGATPTLQCRLNTTTDPPSRSCEAASSCRAT
                     GDGCNADGDCCSGLCVEKTCSTSPVFSRAVYTRIFASNCPSNYKVRWGNLEWHANAPS
                     STHIDFRASVADAPDGFSGGLFLASANMDNVNVPPADLQRVDVGAALEAAEIPSAQYL
                     RITMEFFPSDDGFTAPVLYDWDQRFDCLDAE"
BASE COUNT         2126 a         4246 c         4270 g         2055 t
ORIGIN      
        1 tcatgagtct cgcttggagg tcgacggcgt gttcgacgcc cgaagtcaac gcttcaacgc
       61 cgaccggctg gtggtgttct gcccggccaa gtactgctcg cccgcccgcg acgcaggcgc
      121 tcgcgcgccc gcgcactgcg gctgtccgat ttgctgacga aacgtggagg cggcgccagg
      181 gctacgggtg tcccaagttc cgtcgtcggg cggcgaattc gcttcgctca tgccgtgacg
      241 atcggaagct cgacgacgaa gcgcgcgcct ggtgagtgcg cttcgtcgag cgagatgctg
      301 ccgcccgcgg cctccacgag tcctcggcaa acagcgagac cgaggccagt tcctttgccc
      361 ggatctttgg tcgtggcgaa cggctcgaag agccgaggcc gcatttcgtt ctgcacgccg
      421 gggccgtcgt cggtcacggt gagggagaca gttccttcca cctcgcgcgc cgcgacgacg
      481 atgtgcccac cggctccgac cgcgtcggcg gcgttgagca gcaggttcag caccacttgc
      541 acgagctgtt cgctgccgag cgagacgggc ggcagtgagt cagcgagttc gacgcgaatc
      601 tcgacgccct tcagatcctt ctgaggtgat accagctcga ccgtgtcgtg tacggcacgc
      661 gcgacgtcac ccggcgcggg tcgttcgtcg gctgcgccgg cggcgggacg cgcaaactgc
      721 agcaagtcgc gcaaaatgcg gtgaattcgt tcggtttcgc gacgcatgcg cgtcaagaag
      781 tctcgctctt cggccggctc taggccgccc gcgagcagca gatcttcgag gccgatcagc
      841 gcggcgatgg ggttaccgat ttcgtgcgcg aggccagccg ccaatcgacc caccgaagcg
      901 agccgttcgg agcgcaccag gcgatcttgt gcttcggtga ggcgacgcgt cgcggcctcg
      961 acttcgtcga tcttcttgcg aagcgcttcc tcttcggaga tgagccgttc ggtcatggac
     1021 gcgacgctcg agccgagctc atcgagctct cgcgagcgaa gcagcggcaa cttcaagcga
     1081 cgcgcgccac gcgcgacgcg ctccgcggcg cgtccgagtt cgtcgagggg ccgcacgatg
     1141 aggtaagtga gcgcgaagta cgagagcccc agcagcgaga gcgccacgag cgtcgtgtag
     1201 aggcccaaga ggcgcagcaa cggcgcagcg tgggcggcgt cgtcgtcgat gcgcagcacg
     1261 gccgcgaccg ccgcgtcacc agacggcacg acgacggcga gagctcgccc atgcgcccct
     1321 tcgagctcga acgtcgcctc acggcgaacg tcgaggcgct gagctagctc tggcgaggct
     1381 ttcgcaccgg cgtgcgccag cacgatgcct tcccgcgaaa agatcgaaat cgtctcgacc
     1441 cccgcagttc cgacctcggc tcgcagcaag ccgtccagct cacccggtga ccggcgcgtg
     1501 tgcgcttcgc tcacctgcgc tgccacggca cggccgagcg cgcgcgcgtg accgatccga
     1561 atctgctgaa gggcgacctt cgtgtacgtg ctgacggcag cgtacagggg cacgaaggag
     1621 agcagcagca agccccccaa aagcactaga agttgaaggc ggaggccggg ccgcacgtgc
     1681 cggcagagta accgacctga cctcgcgagg ctcagtttgt gtgcgcgctg gcctcgcgga
     1741 cgcgcgccaa gcaagaacga gccgttctcg aagcactatg aaggatcggg tacgcgcccc
     1801 ggcgctccca ccgctgggcg cgtactcgtg ggccaaaacg cgcgtgaaac ttagccgcgc
     1861 gcctccgacc ctgatagccc cgatttggcc atggatcccc tcgttcggag caccctctgg
     1921 gtgcagcgtc agttgtcgcg aggggccggc actgcgctcg ccacgttgtt cggcggctac
     1981 ctggtcctct gcacgctcga cccgcggcct cgcgccgaag cgttggtggt gctgggagcc
     2041 ttcgtcgcgt cgaccgctta ccgagcctat cagcgcttgc gtcgaccgcc ggccgaagac
     2101 gagagcaccc gcctcgatct ggagctgttc acgcacctcg cggtggcagc gtacgcagcg
     2161 gtgctacggg cgcccggcgg cctcgacggc cccatgtacc ccgtcctgta cgcgctcgcg
     2221 atgttgctcg cgagctttgc gcggcccccc gccgccgccg gcagcctggc tttcgctttg
     2281 ctcctggaaa gcgcgctcca ttacatcgcg ctcgccgagc gcggcgccga acgactgatc
     2341 gctcatggcg tgctgctcgg cgcgttcacg atcctgaact tcgtggtgtt tcgcgccgag
     2401 atcgcgcggg tgcgctcgct ttcccgcctg cgcatcgaga ccgagctcac caagatgcgc
     2461 gaagccgcgc gaacgtatcg actgctcggc gcgcctagcg ctgccagcga gcgctcgcaa
     2521 acgccgccgc ccgacgatcc ggagcgcttg ctacgctctg gggtcgacga gattcaccaa
     2581 gccgtcgcct tcgccctcgg tctgctgcgc cgaacgctcg ggctgcggac ggcgatgctg
     2641 ctcggactcg acgcgaacgg cacgtcgctc agcatccaag agctcgacac cagcgaagac
     2701 gcgattttgc ccggaccgtt cggggcgcgc gacggcatcc tgggagcgag cttggccaag
     2761 tgcgagcaac tctcggtagc cggtccgaag gcgggcctgc acgtccctta ctacgcggtg
     2821 cgtcccagca tcggctcggc gtcggcgact cccttgctcg agcacgggca gccgcgcggc
     2881 ttgctcatcg tggatcgcga atcgcgcgag cccttcaccc gcgacgaaga agagaccttg
     2941 cagcgcgccg cgcgcttctt gctgcgcgcc atcgagaacg agcgcgtctt cgtgcagctc
     3001 gagcgggcca aggtcgaaca ggggaagctg taccgcgccg cgaacgcgct cgcttccgcg
     3061 acgaccgagg cttcggtgat cgaagcttcg gtgaacggcg cgcgcgagtt tgcctcgttc
     3121 gacttcgccg tggtcacgct cttcgacaag gcgacgaacg agcacgaaat ctgtgctgtc
     3181 agcgggcagg gggcgagcga gctcgtgggc cagcgcttcc gtcacaacca gggcctcgtg
     3241 tcgatggtgg tcgcgaaccg gcacgcgctg ccttaccgcg gcgactacga cccttcacgc
     3301 cagatggtgt tcacgcgcag actggcgccg ccgtcgatgc cgtccctcct ggtgctgccg
     3361 ctcttggtgc acgatcgcgc gctcggtacg ctggtcttgg gttcgcagcg ccgcggcgcg
     3421 ttcggcgacg cggtgaggcc gacgctcgag gtgttggcga gccacgtcgc cgtttcgctg
     3481 tcgaacgccc gcatgctcaa gcgcctggaa gagctcgcga ccctcgacgg cctaaccggg
     3541 ctcttcaaca agcgcgcact cacggagctc tcgctccaca agctgcgcag cgcgacccgc
     3601 ttcaagaagg cgctgacgct gctgatctgc gacatcgatc acttcaagaa ggtgaacgac
     3661 acctacggtc acgacgtcgg cgaccaggtc atcaagggtt ttgccgaggt gttgaagcgc
     3721 gtgaagcgcg acaccgacgc ggtcgggcgc ttcggtggtg aagagttcgt ggtgctctgc
     3781 gaggagaccg acgagcgcgg ggccgcgcag cttgcggagc gcattcgcac cgagctcgaa
     3841 gcgacgacct tccactccga gctcggtcag ctcaaggtca cgtgctcgat cggtgttgca
     3901 ccgttccccg cggcgggggc gacttgggag ccgctgttca aagccgccga cgaagcgctc
     3961 tacgccagca aacgtggcgg acgaaaccgc gtcacgatct ggagctccaa gctcggtaac
     4021 gtcgcctgag agcgactact tgccggccgc gtctttggcg atcacgccac gaatcgcggc
     4081 gttcagcgga ttgtcgtcac gcggcggggg cgcggccttc ggtgccggcg caggcttcgg
     4141 gacggcggct ttcgccgggg gcggcgccgg cttcgccttt ggctcgctgg cagcggcgaa
     4201 gcgggcgggg gacggcttcg actcggccac atgggccggc cgcgtcggcg cagcagcgcg
     4261 cgctaccggt gccgcggtgt gggtaactgg cgccgcagcg tgactagcgc cgcgtggtag
     4321 cggggccaac gcatcgagcg acgtaatctt cacgccctcg gaggtcacac cgcccgaggt
     4381 cggagccgcc ggcttcggct cgggcgtcgg cttcgactcg gtcgtcgcct tcggctcggg
     4441 cgtcgccttc ggctctgccg tacgggatgc cgtgggctca gaggctaccg aaggcggagg
     4501 ggacggcgcc gccgccggag tcgcagccgg cgaggccgtg ccgccgaagg ccaccggggc
     4561 tcccagtgcg gcagcggtcg agtccgcgag cgcctccggc gcgagcaacg agcgcggcgt
     4621 gccccacccc ggggttccga gaagacgcct ttccagcgcc aagtaaggcc tttccatccc
     4681 gcgacgccgc gcttcttgga agagcacgtc gttgcgatac agcgaaaaca gcaggcccgc
     4741 gacgcccgcg acccccacca gcgaccacga aaacagatag acggcccgag aaaaccgcga
     4801 accacggctc gagtagcccc cggacgcgta gatgccgaag tcgttgctga ccatgcgcgt
     4861 cgctcatttt agcgtcgtcg cgccgacgtc ggcaacgctg agcattcttc tcgtatctga
     4921 cctcggagct actcgcgcgc cctaccgaaa atcttcgacc cgggcggcac cgactgcgtg
     4981 agccacacgt tgccgccgat caccgaaccc gcgccgatca ccgtttcacc ccccaggatg
     5041 gtcgcaccgg cgtagatcgt cacgttgtct tcgatggtgg ggtgccgctt gtgattccgc
     5101 gaacactcgg ctcggcgcgt gctgagggct ccgagcgtca ctccctggta aatcttcacg
     5161 ttgctgccga tgacgctggt ctctcccacg acgaccccgg tcccgtgatc gaggaaaaag
     5221 cgagggccga tgcgcgcgcc cgggtggatg tcgacccccg tacgagagtg ggcgtactcc
     5281 gaaatgatgc gcggaatgat gggaatcgaa gcggtgaaca actcgtgggc aatgcggtgc
     5341 gcggtgatcg cttcgaccgc ggggtagctg aaaacgatct cttcgatgct cttggcggcc
     5401 ggatcgccct cgaacgccgc caccaaatcc tggttcagcg ttgcccgcac ctcgggcagc
     5461 ttccgaaata gcgtcaggac gacctcttcg gccaagccgg cctcgagtgg cacgccgtga
     5521 aagcggtgct cgtaggcgag cactcgctca atctgctcga ccaggctctc gtacgcggga
     5581 taaaggtgct cgctcagggt gaagcgcaac gagccagagt cgagctcccg agtactgtaa
     5641 aaccccgcgt aaatgacggg gcgaatgtgc aggtaggcct cgacgacggc gctcttgttg
     5701 ggcagcgccg ccgtcgcgag gttgttgatc tcgtcgcggc cctcgtagct cttcgcgatg
     5761 agatcgattg ccgcctcgag ttcccgggtg cgaagcgcca tggtcggaag agcttagcgc
     5821 agaacccgag cgactgctga acagcttgcg gtcaattggt actgacgact gcgcccaagg
     5881 tcacgctgac ggccgaagca gcgcgttcct cttcgtgcac cgctttgggg aaaatctgct
     5941 tgctcgacca gtctgcgcgt ccgtccagaa acacgaccat atgctccgcc gggatgtagt
     6001 cgagcgtgag tgtcccggta cgcacgacga cgtcttccgc gaagccggtc gtatatccgt
     6061 cgggatccgc gtagtgctcg atgcgtcccg caaccccgaa gtgctcgttc acggcgtagc
     6121 gagctcccgc catcacgccc caccaggtcg cggaacgaaa cttcgtgatg tcgccgggat
     6181 cgcgcgctcg atcgatcccg aggctcgcgt tcaaaagcaa cttcagctcc gggatcgggg
     6241 tcgcgacggc ggtcaaatcc aagaagtgac ggaggccctt ggtgttcgac gagccgaggt
     6301 cggccacgcc gcttcctcct tcgctcgcct gcgataggac gcagcctcga gtcggatcga
     6361 agcgctgacc agcgggacac accaaggttt gcgtgtcgcc gtgctcgggc ccgaccaggt
     6421 aacccaccgc gaccgtcagc agatcttcat cgctgccctc ggccttcggc aggcggtacg
     6481 tgccttggag cccgaacgtc ttgccagagt tgttgtcgat ggtgcggttc cagccgttca
     6541 cggccaaaag cttgaggttg aacgagcgcg cagcgtcgat tccgacgcga agcccggtgt
     6601 gatacgcggg ctgaccgagc cagtacagcg cgccgcgcgt gtagttgatg ttgtattgcg
     6661 agtccgcgac ctcggcgccg tacggcgtgt cgaacttgcc gaggtccaac gtgagcgcgt
     6721 cgagcggctt ccacgagacg aacgcctgct tcacgtaaga gaggccgagc gacgactcgc
     6781 tgtcacaggt gtccgcgtcg acgcacgcgc tcgccaggcg ctgcgctcca ggaccgaagc
     6841 gcagcgacaa ggttcccccg acgggcgcag gatccttctg cacgtcgagc ccgacccaat
     6901 tcagcgcgaa gccgttgtgg ttgtcgaagg cgcgcaccgg gttcgagcgg gactgccccg
     6961 gtgatttcgg aaagttgaaa tcgacggctc cgtaggcgtc gacgaacgca gagaacgcga
     7021 ggccctcgta ccaagccgcg ctttcggcga cgggcggcgg cggcgcggcc ggcggcggct
     7081 ccgcgacgga cggcaccgaa ggcggaggag cggcgggcgg cggctcgttc gccggcggga
     7141 tcacggtcgc ggccggcgtc tccggactgg agctcggcgc cgaaggcgca gggtcggtcg
     7201 gcgcaacggg cggcggagtt gctggagccg gttgaactac ggctggcggc accgccgggg
     7261 cggggacagg cgcagccggc gcgggctgag cgactggacc gggcgcgggc gcgggcgcgg
     7321 ggcctggagc gacgggcgcg ggtgccccgg ccgcgggcgg agccggacga atgggcgcgg
     7381 gagcaggttg agccggacgc ggggcgggct gggcggcggc gacttgagcc cacgaggcca
     7441 gcaccgttga gagagcccac gccgaaatcg acccattgct taagcgcttc atccgcgttc
     7501 ctccgcttgt atgtttgccg aacccagtat cacaccccct taaagatccc ttcattttcc
     7561 tacggtcgcg gcgctccgaa ggcgcacgac gcaagaaggg ccgcatgggc cgaaggcgct
     7621 tcagggcggg gtcaggctcg caacgaacac gtcgaggccc tgcgcgccgg gcgccgagta
     7681 atagagacga tcgcactcgg cgttgggcac cgctcgcgct cgctcgccga gcgaaacggc
     7741 tgtttcgaaa gggccgctcg catacgaacg aaacgcggct cgactctcgc gctccgcctc
     7801 gtcccaatag aacaacgtgc gcttgtccgt cgaaatcgcg ctcgggcgac ggaatgagct
     7861 cccactcacc accagcatgt tcccgtcgat gggctcaccg aatggccaag cgtcgcgtga
     7921 acccaggcga ctcgactcgt aaagtgacgg accgccgtcc gctgcagggt cgaggttcga
     7981 gtagacgagc gacagcccat tcggagcgag cacggggtcg ccaaacgggc cccgcgcgcg
     8041 aaaagttccc ggggcggccg cgatctgcac gaacggcgct tcgtcgaaag cccccgcgaa
     8101 agcgtcagtt cgactggcgc gccgcagcaa cccgaacgac tttccgtcct tgcgaaccgc
     8161 gatgagcgaa aggccgtcgc tactcaaagt cgcgccgccg aagtagtcgg ctccccctgc
     8221 gaccggaacg ggcgcgccga acgcatcggt cctcttggcg cgatcggcgt agcggagctc
     8281 tcccgccgcg aaccacgcta tcgagagctc atcgccgctg acgccaacga gttcgtcgtc
     8341 gccggcggcg gagaaagcga ggcgctgccc ctcactccaa ttctgttctg gcgagcacaa
     8401 cggcggcaag ggctcgccac gcgctccgcc gtcgccagcc gcacccgcct cgccggcctc
     8461 gcccacgccg gcatgccctg cgctgccacc tgctccgagc gagcccccgc gcgacgcacc
     8521 gccggaggtg ttggcaaatc ctccgctgct gcgtccaccg ctcgaattcg cttgtggcgc
     8581 gccgggtcgc tcctgctcgg agcaagccgc atgagcgagc gccaccgcga gcgcgaagaa
     8641 ctcgacacga aatcttgcca tgtgaggagc tactcggcgt cgaggcaatc gaagcgctga
     8701 tcccaatcgt agagaacggg cgcggtaaaa ccgtcgtccg aggggaagaa ctccatcgtg
     8761 atccgcaaat actgcgccga ggggatttcc gcggcctcga gcgccgcgcc tacgtccacg
     8821 cgctggaggt cggccggagg aacattgacg ttgtccatgt tcgcgctcgc gaggaacaag
     8881 ccgcccgaaa agccgtcggg cgcgtcggcg acggacgcgc gaaagtcgat gtgagtgctg
     8941 ctcggagcgt ttgcgtgcca ctcgaggttg ccccagcgaa ccttgtagtt gctcgggcag
     9001 ttgctcgcga agatgcgcgt gtacaccgcg cgcgaaaaca ctgggctcgt cgagcatgtc
     9061 ttctcgacgc acagcccgct gcagcaatcg ccgtccgcgt tgcagccgtc gccggtcgcc
     9121 cggcacgagc tcgccgcctc gcagctccgc gacggaggat ccgtcgtcgt gttcaagcga
     9181 cattgcagag tcggcgtcgc tccggtcgag tcgcaacagt cgccatccgc ggtgcacggc
     9241 gcgtgctccc ctttgcaagc agaagggact acgtaggcgc gctcgttcaa atagtgcctg
     9301 ccgaagggcg gctgcgtcgt ttcggcaaag atcgagcgta ggttttgtcc ggggagcagg
     9361 aaagccggat agctccggtc atcgctgccg gcgccggtgt cgtcgagcgc ggccatccag
     9421 agcagcggca tcgcgcaggt gaaatgcgtg ccgacggggt tcagttggtt gccgtaagcg
     9481 cgcgtactcg tgaagacgac ccagcggtag ccaccggccg tgaacggcac cacgttcggc
     9541 tggtacgact tggccgcgtc agctgccaag cgcgtcgcgt cttgatcggc aagcccggcg
     9601 ttcgcgtggg cgagcgcggt gcgcaccaag gtcgtggtgt tgacgctctc gagccgcccg
     9661 ggccagtacc cgcgtgcggt gggcgcgccg ttaccgtgac cgtactcctt ccaatccgac
     9721 cacttgaagc aggcgcgctc gaggtcgctg tcgaccgcga tgccctcggt gctggccgcg
     9781 caatcgggac cggtgcagtg gccctggtac gcttcgagcg gacagaactc gtcggcggcg
     9841 ctcgcttgga agatcacgct gcgactgtcg tgttcgaaga acggccactt gaagacggta
     9901 cccgggaccg agctgtcgta cgaattcagc agccggcgct tgccagagaa cggcacggcc
     9961 gcgttcgcgt tgaagctcaa gagggagagc ccgcgccgcc acccggtcgc aagcgccgtc
    10021 gtcccgaggg tgtccgagtc cccgttgacg tacacgagcg actgaccatc cggcgagaag
    10081 gatggaaaca tcatcgtcgg cagggtgctc ggcgtaccgc cgcggtcgta cttcatccct
    10141 tcgtcgagcg aactcgtgtc gcgcgggaag agcagcgacg cgacacccag gcgctgcact
    10201 ccggtgcctt cgaggacgaa ggtcatgcca ccgaagcggg tgccctgatt gacacgcacc
    10261 cgagcgcccc ccacgagctc gggatccgag tccgcctcgg aacggcgtcg caggacccag
    10321 gggtttgaat tcgcgtcgac gagctcgtaa atgccgttct cgatggcgtt cgcgccgacc
    10381 tcgttcttca cgagcacgga catcgagttc tcgggcggca cgtcgtcgat ccagtccgag
    10441 tcgaaggtgc cgctgccggt caggattggg acaccatcac cccccagcaa gtagttgtgc
    10501 gcaggtagcg ctcgcgacgt cgacgtcatc acggtgtcgt agagcgcgaa ggtgagcggt
    10561 gtgacgtccg gtgtgatcgc gcccatcggc gacgcgaggt ggaagtactt cccggcgttg
    10621 gtgtcgccgt gctcgacgcg aaacttgtcg ccgaagccga ggggcacggc gccgtcctcg
    10681 actctgcgaa ccaatcgaaa tgggctcgaa agccgggaga ccgcaaccgt gtagacaccg
    10741 ttctcgctcg cggtcggctg gtccttcacc aagaccgaat cgcccaccgc gacgccgttg
    10801 ccgtcgatgg tgagcggccc attcgccgtc cccgtcaacg tcccgctcgc gaacgtatgc
    10861 gacggcagcg gcccattcgt cgcaaactgc acgttctttc ggtagtgacg aacgtcgagc
    10921 aagaagtagc gacgtccccc cgcggggtac ccagcgtcct gcagattttc ggacccgagc
    10981 agtacgtcgt gatccgtgtt gccccagaag ttcgcaccct ggagcacgta gcgaccgtcg
    11041 ggtgtcagcg ccgcgtacga gaggcccttc gactgctcgc gctctggctg gatcgagtcg
    11101 ccgcgactgc tcgtccatga atacgtcgga gcgtcggcga tggggttggc gtgcgctcgg
    11161 ccgccgatcg tgtcgagacg agcgatggtc tgtagccccg aagagctgta atcgccggcc
    11221 accagcgtgc gtccgtctgc agaaatcgag tgacacgcgg tgcagcgatt cgccgagctc
    11281 gccccgaacg gatccgtcaa gccgagcgac ggattcgaca gatcgagcgc ctgcagcgtc
    11341 gcgacctgac ttctaaaagt gacggcttgg gtgcagccgc cgacgggaca cgaaggcgcc
    11401 gagttcgcac tgcaatcccc gttgacgcag ccgctcgcgg agtcagtgcg aaaacgcgtg
    11461 tcgcggatgt cgaggtccgg ttgctgaccg tagtcgggat ctccgcagat gttcactgac
    11521 ggcaggttct ggtcgtcgcg aaggcgccgc agatactgcg tgaagaacgc cgtacctcga
    11581 atggagtcgt ttgcgaactt cagcggaatc gccggtagcg gctcgagcgg cgcgggcgcc
    11641 ccctctggat agcgctgcac ctggatgtcg gtgacctggc cagccgctgc gctcgagaac
    11701 gccttccaaa catcgtctgg aattggcgcc gccggctgcg tgctgtcgac caccttcgga
    11761 tcggtcgcac tcgcggccga cgaatacacg cgagaccagt ggaacttcgg agccggtgcg
    11821 ccgcgcggga actcgaggct taccttgacg cagtcggcag tcacgccgcc cgtgctccac
    11881 tgcactagcg gccccggcag accgagaggg aacaccaccg gccgcgcggg aaccgagtac
    11941 gggtacagag tctttccggg atccacccct gacgtcgagc cagaaaagag agtcgcgtca
    12001 cagtcggcgt cgacttcatc gatgttcacg cgaacatcga cggtcgcgct cgcttggccc
    12061 gccgcggtcg ccgccgtgac ttgcaccgtt ttcggcagcg ccgcgagggc gcgcaagacg
    12121 ccgttcccat cgatgcgcgc cacgtcggcg tcgctcgcgc gaaggaccca gctcggctgg
    12181 atgggctcgt ccccgaggcc gcacgtggca gaaaattgga tggtcggagg gtccgttacg
    12241 agcggcgtga ggctcgtgac ggtgatgacc gcgtcgctcg gcgacaggcg cagttgtgcg
    12301 cacgagccgc cgccagcgcg cgccggcaag gtgagcgtgc cgcccttcag cgtgaagtcg
    12361 ttgcctggat cgatgccggg cggcgtatcg atgaacacgc tacagttcgg atcgcacggc
    12421 tcgtgacacg actccggcgt ggtggcgaga gcaagcaggt gcttgtcgcc gctctgcgga
    12481 gcgaacttgg tgacgtggcg agtgacgctg caagcgctcc acacgccgtt cgagcaggtg
    12541 cgcgtcccca tcgcacagac gagatcgtcg ccgctccgcg actcggtctc accacactgc
    12601 gcaaccgctc cttcggaaga gcacgcgcag cctgccgccg tgcagtttcc ccccggctgg
    12661 tttcccgaca cggagacgat gagccccgcg tgatcgt
//
LOCUS       DASTKS010000391         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_509338, whole genome shotgun sequence.
ACCESSION   DASTKS010000391 DASTKS010000000
VERSION     DASTKS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509338"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1519
                     /locus_tag="VFQ35_08120"
     CDS             155..1519
                     /locus_tag="VFQ35_08120"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9930638.1"
                     /translation="MALLKHVVVIGGGFGGLAAARALAKADVLVTLVDRRNHHVFTPL
                     LYQVAMAGLSPAEIAGPIRSVLSKQKNVRILLANATRVDLAARRVELAEAPSLHYDYL
                     VLAAGSRTNYFGHDDWAAVAPGLKDIEDAIEIRRRVLLAFEAAERETDPLAQRRHLTF
                     VVIGGGPTGVELAGAIAELAGYVLSRDFRAIRPEATRVILVEGSPRVLGTFAPTLSDS
                     ARQSLADMGVEVLTGARVTRIDSEGVVFGDTRIEAATVLWAAGVAASPLNQTLGVPLD
                     RAGRVKVDAGCAVPEHPEVFCIGDAALFVPEGSEQPLPGVSPVAMQQGRFVARQIQRA
                     LKKQPPQKFRYLDKGSMATIGRSRAVAEFGKIKLSGFAAWLFWLVVHVYYLIDFRSRI
                     LVLINWAWAYLAYERGARLITGRNIEADDARASDGAGKVLPQPMSPVVQSKPSSSPVA
                     APTP"
     gene            complement(1694..>2446)
                     /locus_tag="VFQ35_08125"
     CDS             complement(1694..>2446)
                     /locus_tag="VFQ35_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930639.1"
                     /translation="IDTRKIVMLRRRGGLSVRPNQTFGAGRLSVINLRTDQELLSSSR
                     ILEKRERELLEAAASLIQTIGSSSLLVSITSPLNLLKELFTVRGAGTLIKPGTIVERR
                     NSYAEVDLTRLTALLESSFGKPLSPLFYERPVAAVFYEPNYRGAAIVLDGGPAPYLSK
                     FAVEPEAQGEGIGNDLWQAMQRDFPRIFWRGRPDNPVTTWYQSVCDGMVRLPNWNLYW
                     RGIAPQQIPNLIAYVEALSPDFGPPPRNEFKP"
BASE COUNT          403 a          749 c          841 g          453 t
ORIGIN      
        1 gcgctgagcg ctcgatggct tcgtcgagct ttcatccacg cgaacgccag gtcgaagacg
       61 agaaatcggc tggctggcga ggtcgagtgg ggcaccatgg gggactgccc aaagtctcgt
      121 cgcgccctat aatgatggca agccggaacg taccatggca ctcctcaaac acgtggtggt
      181 gattggtggc ggatttgggg gcctcgctgc ggcgcgggcg ctcgcgaagg ccgacgtcct
      241 cgtgacgctc gtcgatcgca gaaatcacca cgtatttacg ccgcttttgt atcaagtcgc
      301 gatggccggc ctgtcccccg cggagatagc ggggccgatt cgcagcgtgc tgagtaagca
      361 aaagaacgtt cgcatcttgc tggccaacgc gacccgcgtc gatttggcgg ctcgccgagt
      421 cgagctcgct gaggcgcctt cgttgcacta cgactacctc gtgctcgcgg ccggttcgcg
      481 caccaattac tttggacacg acgactgggc ggcggttgcg cccgggctca aagacatcga
      541 agacgcgatc gagatccgac gacgcgtcct gctcgcattc gaagccgccg agcgcgagac
      601 ggatcccttg gcgcagcgcc ggcacttgac cttcgtggtg attggcgggg ggccgaccgg
      661 ggtcgagctc gcgggcgcga tcgcagagct tgcgggttac gtgctctccc gcgactttcg
      721 agcgattcgt cccgaagcga cccgcgtcat cctcgtcgag gggagcccac gcgtgctcgg
      781 cactttcgcg ccgaccttat cggacagcgc gaggcagagc ctcgcggata tgggcgtcga
      841 ggtgttgacc ggcgcgcgcg tgacgcgaat cgactccgag ggagtcgtct ttggcgatac
      901 gcggatcgaa gccgcgaccg tgctctgggc ggcgggcgtc gcggcgtcgc cgctcaacca
      961 gactctgggt gtgccgctcg accgtgcagg tcgcgtgaag gtcgacgccg gctgcgccgt
     1021 tcccgagcac ccggaagtgt tttgcatcgg ggacgccgcg ttgttcgtgc cagaagggag
     1081 cgagcagcca ttgcccggtg tcagccccgt cgcgatgcag caagggcgct tcgtcgctcg
     1141 gcagattcag cgcgcgttga agaagcagcc gccgcagaag tttcgctatt tggacaaggg
     1201 ctccatggcg accatcggtc gaagccgcgc ggtcgccgag ttcggcaaga tcaagttgag
     1261 cggcttcgcg gcgtggctct tctggctcgt cgtgcacgtc tactatctca tcgacttccg
     1321 cagtcgtatc ttggtgctca tcaactgggc ctgggcgtac cttgcctacg agcgtggcgc
     1381 ccgactcatc accggtcgaa acatcgaggc cgacgacgcg cgcgcgagcg acggcgcggg
     1441 caaagttcta ccccaaccca tgtcgcccgt cgttcaaagc aagccttcgt cttcgcccgt
     1501 cgcagcacca acaccctagc ctctacgggt tgaactcgtt gcgagccagg atcctgcggc
     1561 tgcgaagcgc ggcctcgtcg ctgcgctcct cgaaaggctt cagccttgct ccggtgctca
     1621 ctcctcgggc gcgcttctcg ctcgcgtttc ttggctctcg cgacgggcag gaacttggga
     1681 caccccgtag cctctacggc ttgaactcgt tgcgaggggg aggcccgaag tcgggcgaga
     1741 gcgcctcgac ataagcgatg aggttcggga tctgctgagg ggcgatgccg cgccagtaca
     1801 ggttccaatt gggcagacgc accattccgt cgcacaccga ctggtaccag gtggtgacgg
     1861 ggttgtctgg cctgccgcgc cagaagatgc gcgggaaatc gcgctgcatg gcttgccaga
     1921 gatcgttgcc gatgccttcg ccctgggcct ccggctccac cgcgaatttg gataggtagg
     1981 gggcgggccc gccgtcgagg acgatggctg cgccgcggta gttcggctcg tagaagacgg
     2041 ccgcgacggg gcgttcgtag aagagcggtg acagcggctt gccgaaagaa gactcgagca
     2101 acgccgtgag gcgcgtgagg tcgacctcgg cgtagctgtt gcgccgttcg acgatggtgc
     2161 cgggtttgat gagggttccg gcgcctcgta ccgtgaagag ctctttcaag aggttcagcg
     2221 gggaggtgat gctgacgagg agcgagctcg agccgatggt ttggatgagc gaagccgcgg
     2281 cttcgagcag ctcgcgctcg cgcttttcga ggatgcgcga cgagctcaaa agctcttggt
     2341 cggtgcggag gttgatgacc gagaggcggc ccgcaccgaa ggtttggttc gggcgcaccg
     2401 agagaccgcc gcggcgccgc agcatcacta ttttccgagt gtcgat
//
LOCUS       DASTKS010000392         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1402002, whole genome shotgun sequence.
ACCESSION   DASTKS010000392 DASTKS010000000
VERSION     DASTKS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402002"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFQ35_08130"
     CDS             complement(<1..430)
                     /locus_tag="VFQ35_08130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9930640.1"
                     /translation="MKNRIPALDGVRGLALLLVVLYHVIVFGKVEAVSLPERLLERVA
                     VQGWVGVDLFFVLSGYLITGILRKTRGTEHYYRNFMMRRVLRIFPLYYLFIAVFFLVL
                     PALFPAHHELQRLPPLQAWYWLYVPNVRIFLQGDWYAHYTE"
     gene            complement(693..2534)
                     /locus_tag="VFQ35_08135"
     CDS             complement(693..2534)
                     /locus_tag="VFQ35_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930641.1"
                     /translation="MSVDGSPLRENVVQRLSQLRDLVRMNFDEAMLAHAEWKLKLHLF
                     LHGEGHLDAAVVCRDDACKLGKWLYSDGLKFANDSTYEELRHMHALFHKCAAGVVSTI
                     AQGHKAQAQEQIGHGSEYDALSSEVVDLIAKMKRRVEEKTNLIMGNVLVGILVVDAGL
                     IVQSGYSQHCHELLGARRVAGRHVCDLLGLTDSSAEQFEACMVQAFEDVLPPEVSLAM
                     LPERVRIASRVVGIRASAIRSGGSEVDYALFTLEDVTRVAELEAERAEGHALIRAVRN
                     RHAFSTFARDARLRLASLIEAPLDEPTVRREVHTIKGNAGLFELGEIVSEIDRLEEKL
                     WITHADLSGLAQRLDATIRRFEEALGIGAVEDESSLCTVDQATLTAYETELARSQSDH
                     ERARLLRDLMRRLRMRSVRAILGPVEDRVALLAARLEKVTECHVVGGELLVNERVVAP
                     VVQVLAHALRNAVDHGIECEDERVQRNKAPCGRVELRFEAGADGALLVSLMDDGRGID
                     GRALCRKATSLGLLTEAEADSMSAEQQLELVFLDGFTTAERTTDISGRGVGMSALKAA
                     VAEVGGEIRINSEPGAGTMLQILIPGAADAGERSWQRHSRRASATAV"
     gene            2903..3772
                     /locus_tag="VFQ35_08140"
     CDS             2903..3772
                     /locus_tag="VFQ35_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9930642.1"
                     /translation="MQDLLELVVEARARDLGGFVVGRVLPAPARRALGPFVFLDHMGP
                     ATFAPGQGFDVRPHPHIGLATVTSLFEGEIMHRDSLGFVQPIRPGELNWMTAGRGIVH
                     SERCPDALRASGGELHGLQLWVALPQPAEECEPSFEHVEGEGLPLVDVHGVSVRVLAG
                     EAYGAKASVRVHSRLFYAHAELAAGQSIKLPSDYAERGAYVVSGALQYGTEAHPERRL
                     LVFAPGRVELTASAPTRLALLGGDPLDGARHIEWNFVSSSRERIEQAKRDWRERRFPT
                     IPSDHDEFIPLPD"
BASE COUNT          709 a         1300 c         1197 g          668 t
ORIGIN      
        1 gctcggtgta gtgggcgtac cagtctcctt gaagaaatat ccgcacgttc ggcacgtaaa
       61 gccagtacca ggcctgaagc ggtggcagcc tctgcagctc gtgatgcgcc gggaatagcg
      121 ccggcaacac gagaaagaag accgcaatga acaggtagta gagcggaaag atgcgcagca
      181 cgcggcgcat catgaaattg cggtagtagt gctcggttcc tctcgtcttc cgcaggatgc
      241 cagtgatcag atatcccgag agcacgaaga acaagtcgac ccccacccac ccctgaacgg
      301 cgacacgttc gagcaagcgc tccggaaggc tgacggcctc gaccttaccg aaaacaatca
      361 cgtggtaaag gacgaccaag agcagcgcca aaccgcggac gccatcgaga gcgggaatgc
      421 gattcttcat gagcggggaa ttcggcaagt caccggcaca ccggccgaag cgatactagc
      481 gcgtcgccgt ggagcccaaa ggtaactttc tcgagcttca ttccaaagag ccaggcataa
      541 atgccttggc tctccgcagg gcggagaacg tgggactccc cttgcgtgcg cttggtgctg
      601 gtgcgcgctg gctccctcga gcccacaacc ggccggcctg caacaccgca ctgacgagac
      661 tggtgcggct cggcaccacg ccgcgccgat gctcacaccg ccgttgccga cgcacggcgc
      721 gaatggcgct gccaagatcg ctccccggca tccgccgccc cggggatcag gatctggagc
      781 atcgtacctg cgccgggctc cgagttgatt cggatttcgc ccccgacttc ggccacggct
      841 gctttcaacg ccgacatccc gacgccgcgt ccgctgatgt ccgtcgtgcg ctcggcggtg
      901 gtgaagccgt caagaaatac gagctcgagc tgctgttccg cgctcatgga gtccgcctcc
      961 gcttcggtca acaatccgag cgacgtcgcc ttacggcaaa gcgctctccc gtcgattccc
     1021 cggccatcat ccatcagact cacgagcagc gcgccatcgg cgcccgcctc gaagcgaagc
     1081 tcgacgcgac cgcaaggggc tttgttgcgt tgtacccgct cgtcctcgca ttcgattccg
     1141 tggtccactg cgtttcgaag cgcgtgagca agcacttgga cgaccggcgc gacgacccgc
     1201 tcgttcacca gaagctcacc cccgacgacg tgacactcgg tgaccttctc gagacgcgcg
     1261 gcgagcaggg cgacgcggtc ctcgaccggc cccaggatgg ctcgcaccga tctcattcgg
     1321 agcctacgca tgagatcgcg gagcagccgt gcccgctcgt ggtcggactg cgaacgcgcg
     1381 agctccgtct cgtacgccgt cagcgtcgcc tgatcgaccg tgcagaggga agactcatcc
     1441 tcgaccgcgc cgatgccgag cgcttcctcg aatcgccgaa tcgtggcgtc gagtcgttgc
     1501 gctagtccgg agaggtccgc gtgcgtgatc cacagtttct cctcgagtcg gtcgatctcg
     1561 gaaacgatct ccccgagctc gaatagccca gcgttgccct tgatcgtatg cacttctcgc
     1621 cgaaccgtcg gttcatcgag gggcgcttcg atgagagacg cgagccgtag tcgcgcgtct
     1681 ctggcgaaag tcgagaacgc gtgacgattg cgaaccgcgc gaatcaaggc gtgcccctcg
     1741 gctcgctcgg cctcgagttc cgctacgcgc gtcacgtcct ccaatgtgaa aagcgcgtag
     1801 tcgacctcgc tcccgccgga gcgaatggca ctcgcgcgaa taccgacgac gcgggaagcg
     1861 atacgcaccc gctccggcag catggcgagc gagacctccg ggggcaggac gtcctcgaag
     1921 gcctgcacca tgcacgcctc gaattgttcg gctgaggaat ccgtgaggcc caacagatcg
     1981 cagacatgcc gacccgcgac acggcgagcc ccgaggagtt cgtgacagtg ctgcgagtac
     2041 ccggactgca cgatgagccc agcgtccacg acgagaattc ccaccaacac gttccccatg
     2101 atgaggttcg tcttctcctc cacgcgcctc ttcatcttcg cgatgagatc gacgacctcg
     2161 gaggagaggg cgtcatattc gctgccatgc ccgatctgct cctgcgcctg cgctttgtga
     2221 ccttgtgcga tcgtcgacac gaccccagcc gcgcacttgt ggaagagcgc gtgcatgtgc
     2281 cggagctcct cgtaagtgga gtcgttcgcg aacttcaagc cgtccgagta gagccacttg
     2341 cccagcttgc aagcgtcgtc tcggcagacg acggcggcgt ctaggtgccc ctcgccatga
     2401 aggaagagat gcagcttcag cttccactcg gcgtgggcga gcatggcttc atcgaagttc
     2461 atgcggacga ggtcgcgtag ttgggacaag cgctggacga cgttctcccg aagggggctt
     2521 ccgtctacgg acatggcagt aatccctcct caaaaatcgc acgggttcga tccaggccga
     2581 tgagcgagag cgctggactg cggtctaaag cccccgacaa ggaccggact cactacgtct
     2641 cttggactgc gtttgtacgc taggaacatt gcggccagcc gcgccgcaat ctgaatggtc
     2701 ggcaccacga aacggctcgg tcgagacgga gccttggtgc gactcggcga agcgccgtag
     2761 gcgccttccc cactgccgcc tgagagatct tcgggcggca atcgccacca cgcgctcgct
     2821 caccgaaaga aggcgaagct gcgcggcgaa actggccatc gttctctccg ccccgcttcg
     2881 ttcgacgatg taaagtcgtc gaatgcaaga cttgctcgag ctcgtggtcg aagcccgcgc
     2941 ccgggatctg ggaggcttcg tggtgggtcg ggttctaccc gcgccggctc gacgcgccct
     3001 tgggccgttc gtcttcctcg accacatggg cccggcgacg ttcgcccccg gccagggctt
     3061 cgacgtgcgg ccccatccgc acatcgggct cgcaaccgtg acgagcctct tcgagggcga
     3121 gatcatgcac cgcgacagcc tcgggttcgt ccaaccgatt cgcccgggtg aactcaactg
     3181 gatgacggcc gggcgtggca tcgtgcactc cgagcgctgc ccagatgcgc ttcgagcgag
     3241 tggaggcgaa cttcacggct tgcagctctg ggtggcgctg ccgcaaccgg cggaggagtg
     3301 cgagccgagc ttcgagcacg tcgagggcga aggcttgccg ctcgtcgacg tgcacggcgt
     3361 ctcggtgcgg gtgctcgcgg gtgaagccta cggcgcgaag gcaagtgtgc gcgtgcattc
     3421 gcggctgttt tacgcccacg ccgagctcgc cgcggggcag agcatcaagc tgccgagcga
     3481 ctacgcagag cgcggcgcct acgtggtctc aggcgccctc cagtacggca ccgaggctca
     3541 tcccgaacgc cgtttgttgg tcttcgctcc gggccgcgtc gaactcactg ccagtgcgcc
     3601 aacgcgcttg gctctcctcg gcggcgaccc gctcgacggc gctcgtcaca tcgaatggaa
     3661 cttcgtgtcg agttcacgag agcgcatcga gcaagcgaag cgcgactggc gagagcggcg
     3721 cttccccacc atcccgagcg accacgacga gttcatcccc ttacccgact agggggtgtc
     3781 ccaagttccc cgccctgctg cgagccaaga tcctgcggct gcgaagcacg gcctcgtcgc
     3841 tccgctcctc gaaaggcttc agccttcctc cggt
//
LOCUS       DASTKS010000393         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_509461, whole genome shotgun sequence.
ACCESSION   DASTKS010000393 DASTKS010000000
VERSION     DASTKS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509461"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VFQ35_08145"
     CDS             complement(<1..1845)
                     /locus_tag="VFQ35_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930643.1"
                     /translation="MRTSIFRSFLVVPLVGALAAACSRGDSGEQSTFFSRKIGPVLEQ
                     SCATSPTRSSCHVIADDDLRANALGNLSVESYDHVALRHDLLVNYGPYGIPGLLLKVV
                     PPFKLSLTAWNSTEPVVITTNIAHAGNQLIDFASPTFTTLQRWMANGAAENNAPQAPA
                     VIPQNDCATEIGADSAFDPNTDPTAPDFEQFQSAVNPILGKRCSASNCHGSPANSLRL
                     TCGTTPEQVRWNYFAASDYISKDPSSSELLRRTLAPSAGGTYHEGGTLFPSLDDAGYK
                     AILMWATTKGGPTNVPADAGFPFFAERVQPMLVKRGCMMLGCHSAAMFHDYRLRGGSG
                     GHFGLPATRKNYELSLEQLALESPTPNASRIIRKNLPPDAGGIIHRGGPLFAGYGRPQ
                     ECDLAQAETGPLDQQSPYCVLVAWIAKERAARMASAQALSHIVYVKRNPRPLPDAPQD
                     WETFAPGADLVRAEATLAANGSITLGASSSLSSVCGLDPATSEARRAAVSWDGRRIAF
                     AARTSAGAPYRIYVVDGSSCAVDAAIDAAPVDVDGAALPSNGDLFHNFDPTFAPDGRI
                     IFVSTRGNLQTKTPQIPLGPLRSAADPSKLNTNLYVREADGQIRQLTFL"
     gene            complement(2187..3122)
                     /locus_tag="VFQ35_08150"
     CDS             complement(2187..3122)
                     /locus_tag="VFQ35_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9930644.1"
                     /translation="MSKRLRGLTGLSLLALVLSLPASAAATETDDPNTLGGRAEVYGS
                     LQQSSLEHVTSASALAKELRFQNIAPTRIWKLLEHGEKVECLDCIPRVARLLYDSNAK
                     TREISAWWLRRRIFGVFGKGEVYSQVLGVLGDANETANRRAQAANAIGEFLNPAGVDA
                     VSKAALTDADAPVREASVKALERLNNEGLNQALGKALADRDERVQLAALHAAISVNVF
                     STPELVMERFGDKSALVRRRAAEAVGAMRLSDAVVGLVALSSEANEPSADVRAAAVWA
                     LGQIADPAAKPAVQAALSDSSSTVKNAATIALRRL"
BASE COUNT          511 a         1019 c         1098 g          558 t
ORIGIN      
        1 caagaacgtg agctgtcgga tttgtccgtc ggcctcgcgc acgtaaaggt tcgtgttgag
       61 cttgctcggg tcggccgcgc tgcggagcgg accgagcgga atctgcggcg tcttggtctg
      121 caggttaccg cgcgtcgaga cgaaaatgat gcgcccgtcg ggggcgaaag tggggtcgaa
      181 gttgtggaag aggtcgccgt tgctcggcag cgcggcgccg tcgacgtcga ccggggccgc
      241 gtcgatggcc gcgtccacgg cgcagctcga gccgtccacg acgtagatgc ggtacggagc
      301 gcccgcgctc gtgcgcgcgg cgaaagcgat gcggcgtccg tcccaggaga cggcggcccg
      361 gcgcgcctcg ctcgtcgccg gatcgaggcc gcacaccgac gagagactgc tcgacgcgcc
      421 cagcgtgatg cttccgttgg ctgcgagcgt cgcctcggct cgaacgagat ccgctccggg
      481 tgcgaacgtt tcccaatctt gcggcgcgtc gggcagcggc cgcgggttgc gcttcacgta
      541 cacgatatga gaaagcgcct gagcgctcgc catgcgagcc gcgcgctctt tggcaatcca
      601 ggccaccaac acgcagtaag gcgattgttg gtcgagcggg cccgtttcgg cttgcgccag
      661 atcgcattct tgcgggcgcc cgtatccagc gaagagcggc ccaccgcggt ggatgatgcc
      721 gcccgcgtcg ggaggcaggt tctttcggat gatgcggctc gcgttcggcg tcggagactc
      781 gagagcgagt tgctcgaggc tgagctcgta gtttttgcgc gtggcgggga gcccgaagtg
      841 gccaccgctg cctccgcgca ggcgataatc gtggaacatc gcggccgagt gacagccgag
      901 catcatgcag ccgcgcttga cgagcatggg ctgaacccgc tcggcgaaga acggaaaccc
      961 ggcgtcggcg gggacgttcg tcggcccacc cttcgtcgtc gcccacatca ggatggcttt
     1021 gtagccggcg tcgtcgagcg acggaaacag cgtcccgcct tcgtggtacg tgccgcccgc
     1081 cgacggcgcg agcgtacggc gcaagagctc gctcgaagaa ggatctttcg agatgtagtc
     1141 actcgccgcg aagtagttcc agcgcacctg ctcgggggtg gtgccgcaag tgagtcgcaa
     1201 cgagttcgcc ggagagccgt gacagttgct ggcggagcaa cgcttaccca ggatcgggtt
     1261 caccgcgctt tgaaattgct cgaagtcggg cgcggtgggg tccgtgttcg ggtcgaacgc
     1321 cgagtccgcg ccgatctcgg tggcgcagtc attttgggga atgacggcgg gcgcttgggg
     1381 cgcgttgttt tccgcggcac cgttcgccat ccaacgctgc aaggtcgtga acgtcgggct
     1441 cgcgaaatcg atgagctggt tccctgcgtg cgcgatgttg gtcgtgatca ccaccggctc
     1501 ggtggagttc cacgcggtga ggctcagctt gaagggcggc accaccttca gcaagagccc
     1561 gggaatgcca tacggcccgt agttcaccaa caggtcgtgg cggagcgcga cgtggtcgta
     1621 actctcgacg ctcaggttcc cgagcgcgtt ggcgcgcaga tcgtcgtcgg cgatcacgtg
     1681 gcagctcgaa cgcgtcgggc tcgtcgcgca agactgctcg agcacgggcc cgatcttgcg
     1741 agagaagaag gtgctctgct ctccgctgtc gccgcgcgaa caagcggcgg cgagcgcgcc
     1801 cacgagtgga acgaccaaga acgaccgaaa aatcgatgtt cgcatcagct actgtcgcgc
     1861 gttgggttgg ccccgttgcc ttcgcggcga ctggcggagc cagtatcttc gaggatcgac
     1921 ctgttgctcg ggaaccgaat tggatcgacg gagcacgagc ttatcacgat agcttgcgcg
     1981 attcggcgag ccttcggctc actccttcgg gtctgacaga aggcgctcgc gacgcgcgtt
     2041 accggacgcg aggacgaagc cttggtctgc gggcggcacg cgccgccccg tcctccgaag
     2101 tgaattcgga gagactcccc cgcggcaggt gctttgtgcg gagctgtttt ccgctacgtt
     2161 cggtgaattc ccgatcgttt gccgcgctag agccgccgca gggcgatcgt agccgcgttc
     2221 ttgacggtgg agctcgagtc ggaaagcgcc gcttgcaccg ccggtttggc agccggatct
     2281 gcaatttgcc cgagcgccca gaccgccgcc gcgcgcacgt ccgcggaggg ctcgttggct
     2341 tcggaggaga gcgccacgag acctaccacg gcgtcagaga gccgcatggc tcctaccgct
     2401 tccgcagccc tgcgccgaac gagagccgac ttgtcgccga accgctccat cacgagctcc
     2461 ggggtgctga atacgttgac cgagatggcc gcgtgcagcg ccgcgagctg caccctttca
     2521 tcgcgatcgg cgagcgcctt gccgagcgcc tgattcaagc cctcgttgtt caagcgctcg
     2581 agcgccttga ccgaagcttc gcgcactggc gcgtctgcgt cggtgagggc cgccttgctg
     2641 acggcgtcca cgcccgccgg gttcaggaac tcgccgatgg cattggccgc ctgcgcgcgc
     2701 cggttcgcgg tctcgttcgc gtcgccgagc accccgagta cctgcgagta gacttcgccc
     2761 ttgccgaaca ccccgaagat gcggcgcctg agccaccacg cgctgatctc gcgcgtcttc
     2821 gcgttcgagt cgtagagcag ccgagcgacg cgcgggatgc agtcgaggca ctccactttc
     2881 tccccgtgtt cgagcagctt ccaaatgcgc gtcggcgcga tgttctggaa gcggagctcc
     2941 ttcgccaacg cggacgcgct cgtcacgtgc tcgagcgacg actgttgcaa cgagccgtag
     3001 acctccgcgc gcccgccgag agtgttcggg tcgtccgtct cggtcgccgc ggccgaggcc
     3061 ggcagcgaca ggacgagcgc caagagcgag agaccggtca aaccacgcaa tcgtttagac
     3121 atggcaattg ctccgactca gagagatttc ccggcgacgg ggtcgatcgt gctgggcttg
     3181 aacccg
//
LOCUS       DASTKS010000394         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1402161, whole genome shotgun sequence.
ACCESSION   DASTKS010000394 DASTKS010000000
VERSION     DASTKS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402161"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFQ35_08155"
     CDS             complement(<1..662)
                     /locus_tag="VFQ35_08155"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HET9930645.1"
                     /translation="MRIEDYAIIGDTQTLALVGINGAIDWLCFPRFDSGACFASLLGS
                     EENGRWLLAPKGEATSVRRRYRDGTLILETEFKTPEGTARVVDYMPIRGKNPDVVRIV
                     EDVSGVVPMRSELVIRFDYGSIVPWVRKEHGRLQAIAGPDALVLESPLRHEGRGFTSV
                     SEFEVKPGDRVPFVLTWYPSHERAPESPDPFEALTETERWWRGWASKASDQGEYREQI
                     VRS"
     gene            1566..>2145
                     /locus_tag="VFQ35_08160"
     CDS             1566..>2145
                     /locus_tag="VFQ35_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930646.1"
                     /translation="MRVLVTSLILMSASSLRVCGSEGSGSKAAASAEVAVSANAAATA
                     PLAKASFGGRVVAVGQHSVELALHQSGLVEARVVNADGKLVSDGVALSVAATTKAQGS
                     QEIKLAFVPPKARFEGQAAAGVELTGGPAQITLSVDGKEAKAALQSIAVLKAPEFGGS
                     LLVVGDYGAEVLARADGEVLAFLRDAHGAAVHG"
BASE COUNT          367 a          747 c          684 g          347 t
ORIGIN      
        1 gaacgcacga tctgctcgcg atactctccc tggtcgctcg ccttcgaggc ccagccgcgc
       61 caccaccgct ccgtctcggt cagggcctcg aacggatccg gcgactcggg cgcgcgctcg
      121 tgagacggat accaggtgag cacgaacggc acgcggtcgc cgggcttcac ttcgaactcg
      181 ctgacgctcg tgaaccctcg cccctcgtgt cgcaacggac tttccagaac caaagcgtcg
      241 gggccggcga tggcttgcag gcggccgtgc tcttttcgaa cccacggcac gatgctcccg
      301 tagtcgaagc gaatgacgag ctcggagcgc atcggcacga cgcccgacac gtcttcgacg
      361 atgcgcacca cgtccggatt cttgccgcga atgggcatat agtccacgac acgagcggta
      421 ccctcgggag tcttgaattc ggtctcgagt atcagcgtgc cgtctcggta ccgacgtcgc
      481 accgaagtgg cctcgccctt cggcgccagc aaccagcgac cattttcctc cgaaccgagt
      541 agcgaggcaa agcacgcgcc ggagtcgaag cgaggaaagc acaaccaatc gatcgcccca
      601 ttgattccca cgagcgccaa cgtctgcgtg tcaccgatga tggcgtagtc ctcgattcgc
      661 atggatcgct ccgaagccgc gcccgttgca attcgcattc cggttccgtg cacgccgcgt
      721 gcacctcgct cggaccctgc gtcgcggcgc gaagacgcgc accgagcgcc cgcggccgaa
      781 atcgcggccg cgcactgcgc gaccgactcc gaaaagacgc gtgcacgcgc gccccgcgac
      841 gaccaagttg cccccgcttc gacgcgattc gcgcacgagc gagttgcccc cacgccgcgc
      901 gtcaccccac ggcctgccgt gttcgccttc ggctgcgcgc gccaggggcc cgccaagcgc
      961 tcgcgtccgc tcgcgcaggg gcccgagctc gcagccccgc accaacgcga tggagcgtac
     1021 gcccaacgcc cctctcactt cgcagtgcac cgacgctgcg ctcgaccaat cgtcgtttca
     1081 caccgagggg cacgccagcg acggtcgagc acatggaagc gcgcgagcga agtagagccc
     1141 cccgcgccgc acctttgcct acccgaaaac caccgcgctc tccggcgggg cctctgaggg
     1201 atcccgtcat ccggtgcaaa gttcgccggt tcaaggctga acctcagtcc ttgccgaaac
     1261 gtcgacgagg ctgagcgcgc gctgcaatcg ctgcgagaga gctctcgatg ctcgatttgt
     1321 gtggcattcg cgatcggcca aacgcggctg ccccgcgtcg tcgcttggaa cgagcagaca
     1381 cgagcgccca ccgcaatgcg cggtggactg actttggcag gtgcagttgt cgcacgtcag
     1441 aacaggtgaa ccttcgtctc gttcgttctc cgcgcacgcg aaagggttgg ttagtcggcg
     1501 agctttgcgt gtaacgttgc gacgcgaggc aaaggccgtc gctcgaccca ttggaggctc
     1561 agtcgatgcg agttctggta acgtccctga ttttgatgtc cgcaagctcg ctgcgcgttt
     1621 gcggttcgga aggctctggc agcaaggccg ccgcctcggc agaggttgcg gtgagcgcca
     1681 atgcagcagc gacggcgccg ctcgccaagg cgagcttcgg cgggcgcgtg gtcgccgtag
     1741 gccaacactc ggtggagctc gcactgcacc aatctggtct cgtcgaggcg cgagtcgtga
     1801 acgccgatgg caagctcgtg agcgacggcg tcgccttgtc ggtcgcggca accacgaagg
     1861 cgcaaggctc gcaagagatc aagctcgcgt tcgtgccccc caaagcacgc ttcgaggggc
     1921 aggctgccgc tggcgtcgag ctcacgggcg gtcctgccca aatcaccctc agcgtggacg
     1981 gtaaagaagc gaaggcggca ctgcagtcca tcgccgttct caaggcgccc gagttcggcg
     2041 gcagcttgct cgtcgtcggc gattacggtg cggaggtgct cgcgcgcgcc gatggcgagg
     2101 tgctcgcgtt cctacgcgac gcgcacgggg ccgcggtgca cggcg
//
LOCUS       DASTKS010000395         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1178665, whole genome shotgun sequence.
ACCESSION   DASTKS010000395 DASTKS010000000
VERSION     DASTKS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178665"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFQ35_08165"
     CDS             complement(<1..675)
                     /locus_tag="VFQ35_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930647.1"
                     /translation="MKCPPASVACAFAGLLCALPARSDLGDDAARLSRVWGEHGIVRR
                     LPARFGERGTPSVVFLQIELLSGPASSCLSVVVLGPHSTHFAVRTAGSAYLPDSAEDW
                     PQASLAGLVQITRCGSRKSRLSALVIEMRSPRAVFEVIAVESQQPLEPAVEILPQRDP
                     GPIAPFTGLSLPVAPPPIAERLANAESKARRAGADEVTREQLTTSSRGAGIVRRPVEV
                     GCHRFDV"
     gene            complement(672..1184)
                     /locus_tag="VFQ35_08170"
     CDS             complement(672..1184)
                     /locus_tag="VFQ35_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HET9930648.1"
                     /translation="MDKPWTQGGIRIPATVLDEVERAGREAYARNEEACGYLEGPVSE
                     PLLVDKAVELPNLANKYHAVDPENHPRTGREYFKINALKFERAIAEGTERGRPVKVFF
                     HSHLDCGAYFSAEDAASMTMGGTGDPSFELAYLVTAVDLGKVTAHKLFIWDAKSRTFV
                     EAPFSKVEAA"
     gene            1638..3179
                     /locus_tag="VFQ35_08175"
     CDS             1638..3179
                     /locus_tag="VFQ35_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930649.1"
                     /translation="MLLVLLRCLACLLIIFQALPAAAKPKHRPAPEKVVLEEEPEEAA
                     APPKRSTKTKRAPQPQPEPEPEPEPAPPPAPPPPPPPPPQAVEEEEDESTSGNESSGI
                     ECAPAISEIETRRPLPLSCTTTKKEVAQIELRYKAPGRKKWTKVRLRKQNGEFVGDIP
                     CSALPKAGVLRLSIVGMDGNDKPVARIGGVQIQVVEATNQPPPALPGREPPMRCYDPK
                     ECPPELKGSPACPGTKATPGARSWGASCEKSSQCQQGLACISGSCDKPPACETSSECA
                     SGECVAGHCTFPDPEEVASRLTPKKNWIGLHFGWDFALGKEGNGICGNKTEDGRENWD
                     CYSGSERYKGTPWDYYAGTTAGGLRPSTFRLMASYERWFGRLGTGARLGFAFGGAPKD
                     FQPLHIEARVVYSARPEQLTKRFRPYIGIAGGLARVDSKTKVKIVDCLTTECEKATSQ
                     SQLSTTNARQLTLNAYRKGSGGFVGPTLGFLYAFTSESALQFNVDVMLPDFIIAPTLG
                     YVMGL"
     gene            complement(3300..>3989)
                     /locus_tag="VFQ35_08180"
     CDS             complement(3300..>3989)
                     /locus_tag="VFQ35_08180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9930650.1"
                     /translation="SLIGAALGYRVQLVMPKNVTKARKDITQAFGTELIFSDPMEGSD
                     GAIRLVRKIVSENPEKYFYPDQYSNPSNPLAHYHGTAVEILDALGDRITHFVTGLGTS
                     GTMMGTTRRLHEHTRKIHCVAAEPAEALHGLEGLKHMASSIVPPIYDAGLPDEIMPIT
                     TEEGWDMADRVAELEGLHVGHSTGANIAAAVKLAERLHREQGGGCVVTIACDRGDRYF
                     APLKWERRYVW"
BASE COUNT          714 a         1301 c         1310 g          664 t
ORIGIN      
        1 cacgtcgaag cggtggcagc caacctccac cgggcgccga acgatgcctg cgccacgcga
       61 cgaggtcgtg agctgctcgc gcgtcacctc gtcggcgccg gcgcgtcgcg ctttgctttc
      121 ggcattcgcc aatcgctcgg cgatgggagg cggggcgacc ggcaacgaaa gtcccgtgaa
      181 cggcgcgata ggtcctggat cgcgttgagg caagatctcg accgcgggct cgaggggctg
      241 ctgcgattcc accgcgatga cctcgaacac ggctcgtggc gagcgcatct cgatcacgag
      301 cgcgctgagt cgcgacttgc gagagccgca acgcgtgatc tggacgagcc cggcgagact
      361 cgcttggggc caatcctcag ccgagtcggg gaggtaggca ctgccggcgg ttcgcaccgc
      421 aaaatgagtc gagtgcggcc ccaaaacgac gaccgaaagg cacgagctcg cggggccaga
      481 aagaagctcg atctggagga agacgacgct cggcgtgcca cgctcgccga agcgcgccgg
      541 caagcgccga acgatcccgt gctctcccca aactcgcgac aggcgcgcgg cgtcatcgcc
      601 cagatcagat ctggcgggta gcgcgcaaag gagcccggcg aatgcgcaag caacactcgc
      661 cggggggcac ttcacgccgc ctccaccttc gagaacggag cttccacgaa cgtgcggctc
      721 ttcgcgtccc aaatgaagag cttgtgcgcg gtgaccttcc cgagatccac ggccgtaacg
      781 aggtaggcga gctcgaagct cgggtctccc gtacctccca tcgtcatgct cgccgcgtcc
      841 tccgcgctga aatacgcgcc gcagtcgagg tgcgagtgga agaagacttt caccgggcgc
      901 ccgcgctcgg ttccctcggc gatcgcacgc tcgaatttga gagcgttgat cttgaagtat
      961 tcccgccccg tgcgcggatg attttcaggg tcgacggcgt ggtacttgtt ggcaaggttc
     1021 ggtagctcga ccgccttgtc gaccagcagc ggctccgaaa caggtccttc gaggtatccg
     1081 cacgcctctt cgttgcgcgc gtacgcttct cgtcctgcgc gctcgacctc gtcgagcaca
     1141 gtcgctggga tcctgatgcc gccctgagtc caaggtttat ccatgcaggc aatcctggat
     1201 cacgcgagag acgaaaggga aactccgcgc ttcgaacggg gcgaagaagc gcgcttcagg
     1261 tcggctgccc gggccgcatt cggcgaattt gtggcctctt ttcgaaaagc gggctcagct
     1321 accgcggctt cgatgccgac tcggctccgt cgctcggcga cggcgtgccg ctcgcgcacg
     1381 cgctcatcac gacacctttt gatgccgagc ggtactagac cggaaagatc cagcgaggac
     1441 cgtgagcccc cacgaagtcc tcgcccagga gttggtatcc agatgcggcg acggattcga
     1501 gacacgtctc gattgagtgc agtttcccag tgcacgtgaa acagcttgaa tcacttcacc
     1561 ctccagctta attccccttg agttcgcgag atgcgcgccc cttcttcagg ggctgcgcgg
     1621 tcgtgtgagg aatctcgatg ttgctcgttc tgctccgctg tctggcgtgc ctgttgatca
     1681 tctttcaggc attgcctgct gccgcgaagc ccaaacaccg gccggctccc gaaaaagtcg
     1741 ttctcgaaga ggagcccgag gaggccgccg ctccgcccaa gcgctccacc aagacgaagc
     1801 gcgcgccgca gccgcaaccg gagccggaac cagaacccga gccggcgccg ccgccagcgc
     1861 cgcccccgcc gcccccaccc ccgcctcaag cggtcgaaga ggaggaagac gagtcgacga
     1921 gcggcaacga gagcagcggc atcgagtgcg cgcccgccat ctccgagatc gagacacggc
     1981 gcccgttgcc gctctcgtgc acgacgacga agaaagaagt cgcgcagatc gagcttcggt
     2041 acaaagcgcc gggccgcaag aagtggacga aggttcggct gcgaaagcaa aacggcgagt
     2101 tcgtcggcga cattccgtgc agcgcgctcc ccaaagccgg cgtacttcgg ctcagcatcg
     2161 tcgggatgga cggcaacgac aagcccgtcg cgcggatcgg cggtgtgcag attcaggtcg
     2221 tcgaggcaac caatcaacct ccgccagcgc tccccggacg cgagccgccg atgcgctgtt
     2281 acgacccgaa ggagtgcccg cccgagctca aagggtcgcc ggcgtgcccc ggcaccaagg
     2341 cgacgccggg ggcgcgcagc tggggcgcga gctgtgagaa gagcagccaa tgccagcaag
     2401 ggctcgcctg tatttccggt agctgcgaca agccgcccgc atgtgagacc tcgtccgaat
     2461 gtgcttcggg tgagtgtgtc gcgggccact gcacattccc ggatcccgaa gaggtcgcgt
     2521 cgcggctcac tccaaagaag aactggatcg gcctgcactt cggttgggat ttcgcgctcg
     2581 gcaaagaggg caacggaatc tgcgggaaca agaccgaaga cgggcgcgag aactgggact
     2641 gttactccgg cagcgagcgc tacaagggta ctccctggga ctactacgcc ggaacgaccg
     2701 ccggaggctt acggccctcg acctttcggt tgatggcgtc ttacgagcgc tggttcggtc
     2761 gcctcggcac gggcgctcgg ctcggtttcg cgtttggcgg agcacccaag gacttccagc
     2821 cgctccacat cgaggcgcgc gtcgtgtact cggctcgccc agagcagctg acgaagcggt
     2881 ttcgtccgta catcggaatc gccggcggtt tggcgcgcgt ggactccaag acgaaagtga
     2941 agatcgtcga ttgcttgacc actgagtgcg agaaggccac gtcccagtcg cagctctcga
     3001 cgacgaacgc acggcagcta acgctcaacg cctaccgcaa gggcagcggg gggttcgtcg
     3061 ggccgacgtt aggctttctg tacgccttca cgagcgaatc ggcgctgcag ttcaacgtcg
     3121 acgtcatgct gccggacttc atcatcgcgc ccacgctggg ctacgtgatg ggcctctgag
     3181 cctcgaggat cgtcgcaccg ggggagccgc tgaggcttgg tttccccccg gtcgcggggg
     3241 ccccggcacg agcggggcga gatcgccctc gagcagacgg caacgccgag cgggcgccgc
     3301 taccagacgt agcggcgttc ccacttgagc ggcgcaaagt agcggtcgcc gcgatcgcac
     3361 gcgatggtca cgacgcagcc cccgccttgc tcgcggtgca ggcgctcggc gagcttcacg
     3421 gcagcggcga tgttggcgcc agtcgagtgc ccaacgtgca agccctcgag ctcggcgacc
     3481 cgatcggcca tgtcccagcc ttcttcggtg gtaatcggca tgatctcgtc gggcagcccg
     3541 gcgtcgtaaa tcggcggcac gatggagctt gccatgtgct tgagcccctc gaggccgtgc
     3601 agagcctccg ccggctccgc ggcaacgcag tggatcttcc gggtgtgctc gtgcagcctc
     3661 cgcgtggtgc ccatcatcgt gccgctcgtg ccgagcccgg tgacgaagtg cgtgatgcgg
     3721 tcgccgagcg catcgaggat ctcgaccgcg gtgccgtggt agtgggcgag gggattcgag
     3781 gggttgctgt actgatcggg gtaaaagtac ttctccggat tctcgctcac gatttttcga
     3841 acgagccgga tcgcgccgtc cgagccttcc atcggatcgc tgaagatgag ctcggtgccg
     3901 aacgcttggg tgatgtcttt gcgcgccttc gtgacgttct tcggcatcac cagctggacg
     3961 cggtagccga gggcagcgcc gatgagcga
//
LOCUS       DASTKS010000396         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1848409, whole genome shotgun sequence.
ACCESSION   DASTKS010000396 DASTKS010000000
VERSION     DASTKS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848409"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..930)
                     /locus_tag="VFQ35_08185"
     CDS             complement(28..930)
                     /locus_tag="VFQ35_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930651.1"
                     /translation="MTRTAARDLALQGSEAFDKGQYSAALDLFQRAGALVRAPTISLM
                     QARSLVQLGRWVEALDTYEAAQHVPGIDPGNPAFSAAIASAADEARALKARVPRLKIE
                     LEGSVSPEIEVLLDERKLPSALVGVEMPCDPGEHRVEVRAPNHPALTRTVALTEGTRE
                     LVSIPLVAPPPVQPVELPKPAPPRAAASPPPTTGTDELRTLSWIALIGGGASMGAGLV
                     TSILALDKRSELESVCRPGCPPAYRDDIGQFRTYRNVSYLTIGLGAAAAALGGGYLLF
                     SRRAESHSASLDREPSGVRMVTAF"
     gene            complement(1022..2440)
                     /locus_tag="VFQ35_08190"
     CDS             complement(1022..2440)
                     /locus_tag="VFQ35_08190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930652.1"
                     /translation="MGSSSSSNIHTLALARDAAADSASARSPLEESGDNFYLPGEVIA
                     DRYRLIREIGRGGMGVVWEARSLALGVDVALKLIGKRGAESEAASRMAREAHAAARLA
                     HPSLVRVFDFGWSNRGNPFLVMELVNGETLANVIAKESRIEPIRAVQMLLPVADGLRC
                     AHEKGIVHRDIKPANVLIATDAFGRLQPKLLDFGIARLKQLPIEHRLTQAGVVMGSPA
                     YMSPEQARGDEDVDERSDIWSLCVVLYEMISGEEPFRHTNYNALMQAILHQEPRSTVA
                     LGAGDAQLWQIIARGLAKDHGARWASMTDLGAALATWLYEHGIKEDLCGNSVRALWLD
                     PSQPRRSSDSPVKRVSVPVQADIQTQLRPRRRPRTRSLFFAALLLVPLTAGAALWSRS
                     SRSEALPLPQVDAKSNLKVAQAPLVVPESTRNEPVVTPQPSASAAASAPSAPPPREST
                     RTVRPSSAPRRERNKTVRDFGF"
     gene            complement(2631..2831)
                     /locus_tag="VFQ35_08195"
     CDS             complement(2631..2831)
                     /locus_tag="VFQ35_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930653.1"
                     /translation="MSLAPIVGFAVHRGGGSCGGSRAQHGPAWLQAKDLLQAENSREG
                     GRRAVGSEHAWQLATTRDSAAD"
     gene            complement(2828..>4663)
                     /locus_tag="VFQ35_08200"
     CDS             complement(2828..>4663)
                     /locus_tag="VFQ35_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9930654.1"
                     /translation="NSPVHVHGQGYGDANFIVPEVVRSVRVLEGPYDPRQGDAAIVGS
                     AYFDLAVPARGTTLKGSYGSWNQMRVVGVAAPRGSDEETFAAFSARKTDGFGSKRSSR
                     SASANVQYGLDLSERDHLVLLATAYTARSKLPGVVREDDVEAGVIGNYDAYPFFTRGQ
                     GIQASRVIASASLSRTEPTGTRLEVTPWFMWTDFRARENFTGNIYSSALNPALAGGQG
                     DLWETSNSESALGVVSRFRFAPIRVTSWLEATAEPGVYVRSGHTKQTKSLLNPDTLSV
                     WDRRLNAELATLDTGAYLDLDVRAFRRLRLSGGIRGDFLAVSVENRLGYDVPQNSASA
                     IPGATRSALGVPISPRVTFEYEAVPELTLAASYGEGFRSLGANATVATSDGIAGEGPT
                     IREGGKPYSKVRSLEAGARARTRGDRFSAAVSLFESRVENELVFEASSGGFSTEGASV
                     RRGLVASTLVKPFAWLLGSAAMSISSSTFTTLAPGISHYVPNVPPLLLRADVTARGEI
                     GRFRGEPITARCGVGYTFLAGRHLTDKIVGPSNHVLNAGVAVRRGIVELALDSYNLLA
                     LQYADEAEYYVSNWSVRPGTPLATPAIHLTQAPPLTLLGSLSLYL"
BASE COUNT          774 a         1494 c         1596 g          799 t
ORIGIN      
        1 cgcttgtaac cgcgatcgat cgagtgttca gaacgccgtt accatgcgca ctccgctggg
       61 ctcgcgatcg agcgaggccg agtgcgactc cgctcgtcgg ctgaacaaca agtagccgcc
      121 cccgagcgcg gccgctgctg cgccgaggcc gatcgtgaga taggacacgt tgcggtaggt
      181 cctgaactgc ccgatgtcgt ctcggtatgc gggcgggcag cccggccggc atacgctttc
      241 gagctccgaa cgtttgtcga gcgcgaggat gctcgtgacg agcccagcgc ccatcgatgc
      301 tcctccgccg atgagcgcga tccagctcag tgtcctgagc tcgtcggtgc cggtcgttgg
      361 gggaggggag gcggcggcgc gcggcggcgc cggtttgggg agttcgacgg gctgaaccgg
      421 tgggggtgca acgagcggga tgctgacgag ctcgcgcgtt ccctccgtta gagccacggt
      481 tcgcgtcaac gcaggatggt tcggcgcgcg cacctcgact cgatgctcgc ccggatcgca
      541 gggcatttcg acacccacga gagcactcgg cagcttgcgc tcgtccagca agacctcgat
      601 ctcgggcgaa acgctgcctt cgagctcgat cttgagacgc gggacgcgcg ccttgagcgc
      661 tcgagcttca tccgccgcgc tcgcgatcgc ggcgctgaac gcaggatttc cggggtcgat
      721 gccgggcacg tgttgcgccg cttcgtaggt gtcgagcgcc tccacccagc gccctagctg
      781 cacgagcgag cgcgcctgca tcaggctgat cgtcggagct cgcacgagcg caccggcgcg
      841 ctgaaacaag tccagtgcgg ccgagtactg tcccttgtcg aaggcctcgg agccttgcag
      901 ggccaagtcg cgcgcggccg tccgcgtcac cgggtcggcg tcatcgcctt gcgcacgcgc
      961 tgagagtggc gacagcgcca gagcgcacgc gagagtgaag gccttcgatt tccgcgcgcg
     1021 gctagaaccc gaaatcacgg acggtcttgt tcctttcgcg acgaggcgcg ctcgatggcc
     1081 gcaccgtgcg ggtgctttca cgcgggggcg gggcggaggg cgcagacgcg gctgcgctcg
     1141 cgctgggctg tggtgtgacg acgggttcgt tgcgtgtcga ttcggggacg acgagcggcg
     1201 cctgggcaac cttgaggttc gattttgcgt cgacctgcgg cagaggcagc gcctcgcttc
     1261 gcgaagagcg cgaccacaaa gccgctcccg cggtgagcgg gaccaagagc agcgctgcaa
     1321 aaaaaagcga gcgtgtccgc gggcggcgcc gaggacgcaa ttgcgtctga atgtcggctt
     1381 ggaccggcac gctgacgcgc ttcacgggcg agtcggagct tcgacgcggc tgcgatggat
     1441 cgagccacag cgcgcgcacc gagttgccgc acaagtcttc tttgattccg tgctcgtaga
     1501 gccaagtcgc gagcgccgcg ccaaggtcgg tcatgctggc ccagcgcgcc ccgtggtctt
     1561 tcgcgagccc gcgcgcgatg atctgccaga gctgcgcgtc gccagcgccg agcgcgaccg
     1621 tggagcgcgg ctcttggtgc aaaattgcct gcatcagcgc gttgtagttc gtgtgtcgga
     1681 acggctcttc gccgctgatc atttcgtaga gcacgacgca gagggaccag atgtcgctgc
     1741 gctcgtcgac gtcttcgtcg ccgcgcgctt gctcgggcga catgtaggcg gggctgccca
     1801 tcacgacccc cgcttgggtc agccgatgct cgatcggcag ctgcttgagc cgcgcgatcc
     1861 cgaaatccaa gagttttggc tgcaagcgtc cgaacgcgtc ggtagcgatg aggacgttcg
     1921 ccggcttgat gtcgcggtga acgatgccct tctcgtgcgc acagcgcaac ccgtccgcta
     1981 cgggcaacag catttgcacg gcgcgaatcg gctcgatgcg gctctctttc gcgatgacgt
     2041 tcgcgagagt ctcgccgttc acgagctcca tcaccaagaa cggattgccg cggttgctcc
     2101 acccgaagtc gaacacgcgc acgagcgatg gatgcgcgag gcgtgcggcg gcgtgagctt
     2161 cgcgcgccat gcgggaggcg gcctccgatt cggcgccccg ctttccaatc aacttgaggg
     2221 caacgtcgac tccaagggcg agtgagcgcg cttcccagac gacacccatt cctccccgtc
     2281 cgatttcgcg gatgaggcgg tagcgatcag caatgacctc accagggagg taaaaattgt
     2341 ccccactctc ctccagtggg ctgcgcgcgc tggcgctgtc cgccgccgcg tctcgcgcca
     2401 gcgccagggt gtggatgttg gatgaggagc tactacccat caggttagtc aaaggtcgtg
     2461 acgaactcgg aggcacctgc agtttagcgg tttttcccgc aagtcccacc agcgctcacc
     2521 ggcggccgag ggagaaccgc gcccctgacc gttgctgagc gggtcgaacg gccgcgccca
     2581 ctttccctgc tcgtaacgtt cccaccccgc taccgttacc ggccgcgctt tcaatcggcg
     2641 gcgctgtcgc gcgtggttgc aagttgccag gcgtgctcgc tgccgacggc gcgacgaccg
     2701 ccttctctcg aattttctgc ctgtaacaaa tctttggctt gcagccacgc ggggccatgt
     2761 tgcgcgcgac ttccgccaca gctgccgccg ccccgatgca cggcgaaacc gacgatgggt
     2821 gccaagctca cagatagagg ctgagggatc cgagcagagt gagtggcggc gcttgcgtca
     2881 gatgaatcgc gggggttgcc aacggagttc cgggacgcac gctccaattc gatacgtagt
     2941 actcggcttc atctgcgtat tggagggcga gcaggttgta gctatcgagt gcgagctcga
     3001 cgatgccgcg tctcacggca acgccagcgt tgagcacgtg gttcgaaggc ccgacgatct
     3061 tgtccgtgag atggcgcccg gccaagaagg tgtacccgac gccgcacctc gcggtgatag
     3121 gctcgccgcg aaagcgtcca atctctccgc gcgccgtgac gtcggcgcga agcaggagcg
     3181 gcggaacgtt cggaacgtaa tgtgaaatgc ccggcgcgag cgtcgtgaac gtcgacgagg
     3241 aaatcgacat ggccgccgaa ccgagcagcc acgcgaacgg tttgacgagc gtggacgcca
     3301 cgaggccgcg ccgaacgctc gcgccttcgg tgctgaagcc gcccgacgag gcttcgaaga
     3361 cgagctcgtt ttcgactcgt gactcgaaga gcgacaccgc ggccgagaaa cggtcgccac
     3421 gagtgcgagc gcgagcaccg gcttcgagcg agcgcacctt cgaataaggc tttccgcctt
     3481 cgcgaatggt cggcccttcg ccggcgatgc cgtcggacgt ggcgacggtc gcgttcgcgc
     3541 cgagcgagcg gaagccttcg ccatacgatg cagccaaagt gagctcgggc acggcctcgt
     3601 attcgaacgt gacgcgcggg ctgatgggaa cgccgagcgc cgagcgcgtc gcgccgggga
     3661 tggcgctcgc cgagttttga ggaacgtcgt acccgaggcg gttctcgacc gatacggcga
     3721 ggaagtcgcc gcggatgccg ccgctgagac gcaagcgccg gaacgcgcgt acgtcgaggt
     3781 cgaggtatgc tcccgtatcg agcgtggcga gctcggcgtt caggcgacga tcccagaccg
     3841 acagagtgtc gggattcaat aggctcttcg tttgcttcgt gtggcccgag cgaacgtaga
     3901 ctccgggctc ggccgttgct tcgagccagg acgtcacccg aatcggcgcg aagcgaaatc
     3961 gcgagacgac gccgagcgcg ctctcgctat tcgaggtttc ccagaggtcg ccctgcccgc
     4021 ccgcgagcgc agggttcagc gccgagctgt agatgttgcc ggtgaagttc tctcgagcgc
     4081 gaaagtcggt ccacatgaac caaggggtga cttcgaggcg tgtgcccgtg ggctcggttc
     4141 ggctcaggga ggcgctcgcg atgacgcgag acgcttgaat gccctgacct cgggtgaaaa
     4201 acggatacgc gtcgtagttt ccgatgaccc ccgcttcgac gtcgtcttcg cggaccacgc
     4261 cgggcagctt cgagcgcgcc gtgtacgcgg tcgcgagcag gacgaggtgg tcgcgctcac
     4321 tcagatcgag gccgtactgc acgttggcgg aggccgaacg ggagctgcgc ttcgagccga
     4381 agccatccgt cttgcgagcg gagaacgccg cgaacgtctc ttcgtccgag ccgcgcgggg
     4441 cggcgacgcc cacgacgcgc atctggttcc acgagccgta gctccctttg agcgtcgtgc
     4501 cgcgcgcggg aacggcgaga tcgaagtatg cgctccccac gatcgcagcg tcgccttgcc
     4561 gtgggtcgta cggcccttcc aaaacccgta cgctgcgcac gacctcgggg acgatgaagt
     4621 tagcgtcgcc gtagccttgg ccgtgcacgt ggacggggct gtt
//
LOCUS       DASTKS010000397         6417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_732042, whole genome shotgun sequence.
ACCESSION   DASTKS010000397 DASTKS010000000
VERSION     DASTKS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732042"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFQ35_08205"
     CDS             complement(<1..509)
                     /locus_tag="VFQ35_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930655.1"
                     /translation="MDPAFERFVTDASASCRTATGEYVPGPDTGTPVICQPDNLRFKR
                     FVNQLGFAFAPSAMHSARTTGYGGFDVGIEGTYTKISSGKSYWKLGTQGSGDPSTNLA
                     AVQNNSPESLLQLYSLRVRKGFGFGLEVSGMVGFMPKTSLLGGGADVRLSLLEGFRRG
                     LLGIFPDIAV"
     gene            763..1254
                     /locus_tag="VFQ35_08210"
     CDS             763..1254
                     /locus_tag="VFQ35_08210"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET9930656.1"
                     /translation="MTRPLHIVLVEPEIPPNTGNIARLAAATGCPLHLVGPLGFRIDA
                     HAVRRAGLDYWEHVELHQHRTLSDAEQNLSYGESAGRTYLFSGRTERSFLDVAFRFGD
                     RLVFGKESVGLDEALLTSRPEDVVGIPMLGPVRSLNLANAVSIALYEALRQLGVLKNA
                     RLT"
     gene            complement(1258..3009)
                     /locus_tag="VFQ35_08215"
     CDS             complement(1258..3009)
                     /locus_tag="VFQ35_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930657.1"
                     /translation="MFPQVFGKYVLDRELASGGMARVYLATLRGAVGFEKRLVVKQIR
                     PELASDPAFVRRFVDEAKTAVELSHPNIVPVYELGVEQGVYYIAMEFCAGATLAELLS
                     EAGAISPEEGAYLGVEICRALDYAHRRAGIVHRDVTPRNVLIDEEGAVRLIDFGIAAP
                     VAEHGPQEIFGSPGHMPPEQLAGKKLTPATDVFAVATLLVEAWTKKPPFRRATARASA
                     EALQEQRPRLEELIPELAPLAELIERGLSLDAEQRPAQAELFARPLRDFLRSADAGDV
                     ARRLGERVRKVHGRAGRRSSHGEGTAALPARGESSRASDAGISAPTYTFAARGEVAEW
                     TRRLEDSAKGGSGSGGPSTRKLASIPPEEPASVEPVLDGVELSGESAPSSVPESAGPG
                     RSPAPGEQRRARPVRLAGFALGAVFLASALVLLASSPRSAVTSARGARPVTAVLIPST
                     APPTAPSAVVAEFREVRPPEPVEAPPRSVPPARSSQPSSTPKRASANDATPVASARLR
                     LTADPPASVSLQGASGLRAFTTPVRELSVPPGVYVLTFRNDTYGMPVVARVNMEAGGE
                     RSVHVDFREVEPRVTVR"
     gene            3080..4978
                     /locus_tag="VFQ35_08220"
     CDS             3080..4978
                     /locus_tag="VFQ35_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930658.1"
                     /translation="MLIVLLALAQVRCEDVPKERTLALTTHAPAGCERRPSPGDASLR
                     LTPLGDFALPAASEVLLDTAKSLDFPEQTRAVVAEARTSDGVFVGYAARGAKPSLDVL
                     LWPDEAACRLDVDPGAAPNPGGGQALGVSADATLALIVGGNSPKLPAGSVAATIFDTG
                     SGEVTALSSSGEPRTALAEPRAFATVTPFGTQMLVAGGEDPLNSDGNGLAPPSKTAEV
                     FDPERRRFGSALTLTVARSRHAAVALAGGQTLLIGGRGPSGNALNALEVLAPGDTHGS
                     IQGLAALTYPRLFPTAALLDDGRIFVGGGVTALGTPLSALEWLSSNAKQILRADLPEQ
                     LPPRFDRAFAALPGGGVLAVGGCQPSEDPCTDVCRSGCPPRDAGDDRVRYDAWWIAPD
                     GALTRVPLDIDAPRPVLLGGGDGRPMLASGAPGDTKLYRFDPWQARFVEGPFSVSAPP
                     VAGLPATAPDDGAFLWLSEDGVGAQLSGARLSTRNRFAIDENLLGAPSDDVAAPYPLV
                     PDRPALSKTRFDDSVNTLFFEEGSDVGVFVAASDYANVTVELTVEGDAPKVVLGTREY
                     GGDDCPWPIGAVAQFRLERRGERVTLGSAGTSAPSCDAPRGRVRLGVKRGRGAMGVRY
                     FRVTREPE"
     gene            complement(4982..>6417)
                     /locus_tag="VFQ35_08225"
     CDS             complement(4982..>6417)
                     /locus_tag="VFQ35_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9930659.1"
                     /translation="GRVLVRDGYIDETALRAGLREQLAKQIVYLAGRPADTLYGYYDG
                     VDLLERWGGPAVRGRPLNVIWRVIAARSEPGRVSDVVGRLGDREIRLHNDAPIQRFHF
                     GRDEKPLIELLRAKPQSLKELVARDIADRALVERLVYALTITRQIELGTHVLPLGVDE
                     PPSSIRPVAMPQAPAFGDTAYPLRGTGVSGAMQSVRPTPSPAPAVVESPEIVAFRAEI
                     LERGARESDTLYELLGVARDVAPADIQPAFLSLAKKWHPDRLPAELASLKETVTRIFA
                     RMSEASQVLSDAMQRREYDSKLRRAGREAEEQEHVQRVLRAATALQKADVFLKRNNLA
                     AAEVEARSALADDPDQSDCIALVAWIDAQKPDANLDNCIKQLDRAIMIQPTSVRAHWY
                     RGQLHKRAGRTGRAIRDFRLVVEKDPRHTDALRELRLFQMRRGDRSTSIPPSGGENSR
                     PSPPPAGPDKKPTPPQGGGFISKLFKR"
BASE COUNT         1022 a         2196 c         2184 g         1015 t
ORIGIN      
        1 actgcgatgt cggggaaaat gccgagcaag ccgcgccgaa agccttcgag cagcgagaga
       61 cgcacgtccg cgccaccgcc gagcaggctc gttttcggca tgaagccaac catgcccgac
      121 acctcgagcc caaagccgaa gccctttcgg actcgcagcg aatagagctg cagcaggctc
      181 tcggggctgt tgttttgcac cgccgcgagg ttcgtcgatg ggtcacccga cccttgagtg
      241 ccgagcttcc aatacgactt gccgctcgaa atcttcgtgt acgtgccctc gatgccaacg
      301 tcgaaaccac cgtacccggt ggtgcgcgcg gagtgcatcg cgctcggtgc gaacgcgaag
      361 cccagctgat tgacgaagcg cttgaagcgc aagttgtcgg gctggcagat cacgggcgtc
      421 ccggtgtccg gaccgggaac gtactcgccc gttgccgtac ggcagctcgc gctcgcgtcg
      481 gtcacgaagc gctcgaatgc cggatccatt ggctcggcgg aggccgaccg agccgcacct
      541 agagccaaca acccgagagc gagtactgcg gggcgagctt tcattgtccg cgggccattt
      601 cgaccgccga gggaggctac ttgccgcccg gcaggcccgt caagacaacc tcgggggtgc
      661 acgccgcacc ggcccggggt ccaccggcgc ggcagctctt cccgccccgt gctacgcggc
      721 ggcgtgcccg ctgacaatgc cgagagactg cgcgcgcgtg ccgtgaccag gccgctccac
      781 atcgtcctgg tcgagcccga gattcctccc aacaccggca acatagcgcg cctcgccgcg
      841 gcaacggggt gcccccttca cctggtcggg ccgctcggct ttcggattga tgcacacgcg
      901 gtgcgccggg caggcctcga ctactgggag cacgtcgagc tgcaccaaca caggaccttg
      961 tccgacgccg agcagaacct ctcgtacggc gagtcggcgg ggcgcacgta cctcttcagc
     1021 gggcgcacgg aacgaagctt cctggacgtc gcattcagat tcggagatcg cttggtgttc
     1081 ggcaaagaga gcgtcgggtt ggacgaagcg ctgctcacct ctcgtcccga ggacgtggtg
     1141 ggcattccga tgctcggacc ggtgcgttca ttgaacctcg cgaacgcagt gtcgatcgcg
     1201 ctctacgaag cgctgcgcca gctgggtgtg ctgaagaacg cgcggctcac ttgaacttca
     1261 gcgcacggtg acgcgcggct cgacctcgcg aaaatcgacg tgaacgctgc gctcgccgcc
     1321 cgcctccatg ttgacgcgcg cgaccaccgg catgccgtaa gtgtcattgc ggaaagtgag
     1381 cacgtatacg ccgggcggca ccgagagctc tcgcacgggc gtcgtgaaag cgcgcagccc
     1441 cgaggcaccc tgcaagctga cgctcgcggg cggatcggcc gtcagccgca accgggcgct
     1501 cgccaccggg gtcgcgtcgt tcgcgctcgc acgcttgggc gtcgaggaag gttgcgacga
     1561 gcgagcaggg ggaaccgagc ggggtggagc ttcgaccggt tctggcgggc ggacttcgcg
     1621 aaactccgcg acaacggcgc tcggcgcagt cggcggcgcc gtcgacggga tgagcacggc
     1681 agtgacggga cgagcgccgc gcgcggaggt aaccgccgag cggggggaag aagcgagcag
     1741 cacgagcgcg ctcgcgagaa atacggcacc caacgcaaat cccgcgagcc gcacgggccg
     1801 ggcgcgccgc tgttcgccgg gcgcagggga ccggccagga cctgcgctct ccggcaccga
     1861 cgacggcgcg ctctctccgc tcagctccac tccatcgagg accggctcga ccgaggctgg
     1921 ctcctctggc ggaatcgatg cgagcttgcg ggtggacggg ccgcccgaac ccgagccgcc
     1981 cttcgccgag tcttccagcc ggcgagtcca ttccgcgact tcgccgcgcg ccgcgaaggt
     2041 gtaggtcggc gccgaaatac cggcgtcgga ggcgcggctc gactcgccgc gcgcgggcaa
     2101 cgccgcggtt ccctcgccgt gagacgagcg ccgcccggca cgcccgtgga cctttctcac
     2161 gcgctccccg agcctgcgcg ccacgtcgcc ggcgtcggcg ctgcgcaaga agtcgcgaag
     2221 aggtcgcgcg aagagctccg cctgcgcggg gcgctgctcg gcatcgagcg agaggccgcg
     2281 ctcgatgagc tcggcgagtg gagcgagctc cgggatcagc tcttcgagac gcggccgttg
     2341 ctcctgcaac gcttcggcgc tggcgcgcgc ggtcgcgcga cgaaacggcg gcttcttcgt
     2401 ccaggcttcg acgagcaggg tggccactgc gaaaacgtcg gtcgctggcg tcagcttttt
     2461 tccggcgagt tgctccggcg gcatgtggcc gggcgagccg aagatctctt gggggccgtg
     2521 ttcggcgacg ggcgcggcga ttccgaaatc gatgagccga actgcacctt cctcgtcgat
     2581 cagcacgttc cgcggggtca cgtcgcgatg cacgatgccg gcgcgccggt gcgcgtagtc
     2641 gagcgcgcga catatctcga cgccgaggta cgcgccctct tcgggtgaaa tcgctcccgc
     2701 ctcgctgagg agctcggcga gcgtcgcgcc ggcacagaac tccatcgcaa tgtaatagac
     2761 gccttgctcg acgccgagct cgtagacggg aacgatgttc gggtgcgaaa gctcgacggc
     2821 agtcttggcc tcgtcgacga agcgacgcac gaacgcgggg tcggacgcga gctcgggccg
     2881 gatctgcttt acgacgagtc gcttttcaaa acccaccgcg ccgcgcaacg tggcaaggta
     2941 cacgcgcgcc atgccgccgg aggcgagctc gcgatcgagc acatacttcc cgaatacctg
     3001 ggggaacacg agggagctcg ccgctgggaa gaatgcgccc agcggacgtt cttcacatgc
     3061 tacccctagt tcttcgggcg tgctcatcgt gctgctcgcg ctcgcgcagg taaggtgcga
     3121 agacgtaccc aaagagcgca ccctcgcgct gacgacgcac gctcccgctg gctgcgagcg
     3181 ccgcccttcg ccgggcgatg cttcgttgcg actgacaccg ctcggcgact tcgctcttcc
     3241 agccgcgagc gaagtgctgc tcgataccgc gaagagcctc gatttccctg agcagacgcg
     3301 cgcggtcgtg gccgaagcgc ggacttcgga cggggtcttc gtggggtacg cagcgcgcgg
     3361 ggcaaagcct tctctcgatg tgttgctgtg gcccgacgag gccgcctgcc gcctcgacgt
     3421 cgacccggga gcggcgccga accctggcgg aggccaagca ctcggggtca gcgcagacgc
     3481 aaccctggcc ctcatcgtgg gaggtaactc gccgaagctt ccggcaggca gcgtcgcggc
     3541 aacaatcttc gacaccggca gcggggaggt gactgctctc agctcgagcg gagagccgcg
     3601 cacggctctc gcggagccgc gggccttcgc gaccgtgacg cctttcggaa cccaaatgct
     3661 cgtggccggc ggtgaagacc cgctgaacag cgacggaaat ggcctcgccc ctcccagcaa
     3721 gaccgccgaa gtattcgatc ccgagcgccg acgcttcggc agcgcgctca cgctgaccgt
     3781 cgcccgttca cggcacgcag ccgtcgccct cgccggcgga cagacgctgc tcatcggcgg
     3841 gcgtggcccg agcggaaacg ccctgaatgc tctcgaggtc ctggcgcccg gcgacactca
     3901 cggcagcatt caagggctcg cagcgctcac ctatccccga ttgtttccga cggccgctct
     3961 tctcgacgac ggtcgaatct tcgtgggagg cggagtcacc gcgctcggaa cccccctctc
     4021 cgcgctcgag tggctttcgt cgaacgccaa acaaatcttg cgcgcggacc ttccggagca
     4081 gctgccgcct cgcttcgacc gcgccttcgc ggcgctgccc ggcggcggcg tgctcgcggt
     4141 cggcggctgt cagccaagtg aggacccctg caccgacgtc tgtcgctccg gctgcccgcc
     4201 gcgcgatgcg ggcgacgacc gcgttcgcta cgacgcgtgg tggatagcgc cggacggcgc
     4261 tctcactcgc gttccgctcg acatcgatgc gccgcgcccg gtgctgctcg gcggaggcga
     4321 cggtcgtccg atgctcgcga gcggtgcgcc cggagatacg aagctatatc gcttcgatcc
     4381 gtggcaagcg cggttcgtcg aggggccttt cagcgtctcc gcgccgcccg tcgccgggct
     4441 acccgcgact gctcccgacg atggcgcgtt cttgtggctg agcgaagacg gcgtcggggc
     4501 gcagctctcg ggcgcgcggc tcagcactcg caaccgcttc gccatcgacg aaaacctgct
     4561 cggcgcgccc agcgacgatg tcgccgcgcc ctatcccctg gtgcccgatc ggccagcgct
     4621 atccaagacc cgcttcgacg acagcgtgaa cacgctgttc ttcgaggaag gcagcgacgt
     4681 cggtgtcttc gtcgccgcga gcgactacgc gaacgtcacg gtggagctca cggtcgaggg
     4741 cgacgcgccg aaagtggtac tcggcactcg tgagtatggc ggggacgact gtccgtggcc
     4801 catcggagcc gttgcccagt tccggctcga acgccgtggc gagcgagtca cgctcggctc
     4861 ggcgggcacc tcggctccgt cgtgcgacgc tccgcggggg cgcgtacggc tcggcgtcaa
     4921 gcgcggtcgc ggcgcgatgg gcgtgcgcta ctttcgcgtt acacgcgagc ccgaatgaac
     4981 gtcagcgctt gaagagcttg ctgatgaacc caccgccctg gggcggcgtc ggcttcttgt
     5041 cgggaccagc gggcggaggg ctcggccgcg agttctcgcc gccggaaggc gggatgctgg
     5101 tgctgcggtc gccgcggcgc atctggaata gtcggagctc gcgcagcgcg tccgtgtgcc
     5161 gaggatcctt ctcgactact agacgaaagt cgcgaatggc gcgaccagtg cgcccggcgc
     5221 gcttgtgcag ctgacctcgg taccaatggg cgcgcacgct ggtcggctgg atcatgatcg
     5281 cgcggtcgag ctgcttgatg cagttgtcga ggttcgcgtc cggcttctgg gcgtcgatcc
     5341 acgcgacgag ggcgatgcag tccgactggt ccggatcatc ggcgagtgcg cttcgagcct
     5401 cgacttcggc tgcggccagg ttgttgcgct tcaggaatac gtcggccttc tgcagtgccg
     5461 tcgcggcgcg cagcacccgc tgaacgtgct cttgttcttc cgcctcgcga ccagcgcgcc
     5521 gaagcttcga gtcgtactcg cgacgctgca tggcatcgct cagcacctga ctcgcctcgc
     5581 tcatgcgcgc gaagatccgc gtcaccgttt cttttaggct cgcgagttcg gcgggcaagc
     5641 gatcggggtg ccacttcttc gcgagggaca agaacgcggg ctgaatgtcg gccggcgcaa
     5701 cgtcgcgcgc tacgcccaag agctcgtaca gggtgtcgct ctcgcgcgcg ccgcgctcca
     5761 gaatttcggc tcgaaaagcg acgatttcgg gggattccac caccgccgga gccgggctcg
     5821 gagttggtcg caccgactgc atggcgccgg agacgcccgt gccgcgcagc gggtaggcgg
     5881 tgtcgccgaa agccggcgct tgcggcatcg ccaccggtcg aatcgacgaa ggcggctcgt
     5941 cgacaccgag cggcaacaca tgcgtgccga gctcgatttg tcgggtgatg gtgagcgcgt
     6001 agaccaagcg ctcgaccaaa gcgcgatcgg cgatgtcacg agccacgagc tccttcaagg
     6061 actgcggctt cgcccggagt agctcgatga ggggcttttc gtcacgaccg aagtgaaatc
     6121 gctgaatcgg cgcgtcgttg tgcaagcgga tctcgcgatc gccgagtcgc ccgactacgt
     6181 cgctgacgcg cccgggctcc gagcgcgctg cgatcactcg ccaaatgacg ttgagcggac
     6241 ggccgcgcac cgccggaccg ccccagcgct cgagcaaatc gacgccgtcg tagtaaccgt
     6301 acagagtgtc cgccggacgc cctgccaaat agacgatctg ctttgcgagc tgctctcgca
     6361 acccggcccg cagcgcggtt tcgtcgatgt aaccgtctcg aaccaagacg cgcccgt
//
LOCUS       DASTKS010000398         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1178821, whole genome shotgun sequence.
ACCESSION   DASTKS010000398 DASTKS010000000
VERSION     DASTKS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178821"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="VFQ35_08230"
     CDS             <1..1738
                     /locus_tag="VFQ35_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9930660.1"
                     /translation="ATATIGNPREHAARLIGTEPDQVTLVDKSGAPTAAREVYLYNPP
                     IVNAELGVRGSTLKHAVSLAADLVKARVPTIVFGPSRNSVEVMLKYLRAKASDVAGPD
                     AIMGYRGGYLPETRRRIEAGLRSGEILAVVATNALELGIDIGDLDAVICAGYPGSVAA
                     TWQRFGRAGRRGERSIAVMVCSSSALDQYLAREPKYLFEAGAEEARIDPGNVEVSIQH
                     LKCAVFEAPFELNARGPRPANPTVAAGETHYGLNPESTRDALDFLAGHGLVHQSEGRY
                     HWAGEAFPASSVSLRNIGWDNFVIIDVATEKTLAELDYRAAHTMLHEQAIYQHDAEQY
                     QVERLDFDNHKAFVRKVAPDYFTTALTYRTVSVLEEAAQQPLGRGSIGHGDVKVLERV
                     TGYKKIKFFTHENAGYGDVHLPEMQMHTTSFWLTVPEAIVSELGEPRALVVDALRGLG
                     AALETVSALALMCDPRDLGQTLGDGSNPNGDGGVMIPAGRDFFAGRTGRFDPTVFLFD
                     AHAGGVGLAPRIYERAEELLGRVRTLLAGCPCEAGCPACVGPSDPSGVRKRLALRLLA
                     ALAAAPLRAEAV"
     gene            1917..>2672
                     /locus_tag="VFQ35_08235"
     CDS             1917..>2672
                     /locus_tag="VFQ35_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET9930661.1"
                     /translation="MVRAAGFFSVAALFALGCRGTGPHINRVVDNEDAGTGIDLTPDA
                     GAKDDASSPQAEPHALLSADPAHGPFAGGTLVLLRGNGFASNARVWFGANAVPAGDAL
                     PVDSHRIQVTAPAGEPGPTDLVVQNGDDESTRVTLRGGYTYDGYYAEPNSGPTSGGTL
                     VTLHGYGTSWDATTSVTIDRNPCEVVSVDDPTTLTCRAPAGTPGAKPIRVTTGDGASI
                     DVLDGFTYSNSDNGFRGGLSGAPLGDELKVLVFG"
BASE COUNT          465 a          893 c          891 g          423 t
ORIGIN      
        1 ggcgacggcg accatcggca acccgcgcga gcacgcggcg cggctcatcg gaacggagcc
       61 ggaccaggtg acgctcgtcg acaaatccgg cgcaccgact gcggcgcgcg aagtgtacct
      121 ctacaaccct cccatcgtga acgccgagct cggcgtgcgc ggcagcacgt tgaagcacgc
      181 ggtgtcgctc gccgcagatc tcgtgaaggc gcgcgtgccg accatcgtgt tcggtccctc
      241 gcgaaattcg gtcgaggtga tgctcaagta tttgcgcgcc aaagcctccg acgtcgctgg
      301 gcccgatgcg atcatgggct accgcggagg ctacttgccg gagacgcgcc ggcgcatcga
      361 agctggcctt cgcagcggcg agattttggc ggtcgtcgcg acgaacgcgc tcgagctcgg
      421 tatcgacatt ggcgatctcg acgccgtcat ttgcgccggc tatcccggct ccgtggcggc
      481 aacgtggcag cgcttcggcc gcgccggccg ccgtggcgag cgcagcatcg cggtgatggt
      541 gtgctcgagc tcggcgctcg accaatacct cgcgcgcgag cccaagtacc tgttcgaagc
      601 gggcgccgaa gaagcgcgca tcgatcccgg caacgtcgaa gtctcgattc agcatttgaa
      661 gtgcgccgtc ttcgaagctc cgttcgagct caacgcgcgc gggccgcgcc cggcgaaccc
      721 gaccgtcgcc gcgggggaaa cgcactacgg tctcaacccc gaatcgacgc gggacgcgct
      781 ggatttcttg gcggggcacg gcctcgttca tcagtccgaa gggcgttacc actgggccgg
      841 cgaggccttc cccgcgagca gcgtctcgct ccgaaatatc ggttgggaca acttcgtcat
      901 catcgacgtc gcgaccgaga agacgctcgc cgagctcgat taccgcgccg cccacacgat
      961 gttgcacgag caggccatct accagcacga cgccgaacag taccaagtcg agcgcctcga
     1021 cttcgacaat cacaaggcgt tcgtgcggaa ggtcgcgccc gactacttca ccaccgccct
     1081 cacgtaccgc accgtgagcg tgctcgaaga ggcggctcag caaccgctcg gtcgcggcag
     1141 catcggccac ggcgacgtaa aggtgctcga gcgcgttacc gggtacaaga agatcaaatt
     1201 ctttacccac gagaacgcgg ggtacggcga cgtgcacttg cccgagatgc aaatgcacac
     1261 gacgagcttt tggctcaccg tgcccgaagc gatcgtgtcc gagctcggag agccgcgagc
     1321 cctcgtcgtg gacgcgctgc gaggcctcgg tgcggcgctc gagacggtct cggcgctcgc
     1381 gctcatgtgc gatccgagag atttgggaca gacgctcgga gacggctcga acccgaacgg
     1441 cgacggcggc gtgatgatcc cggccgggcg cgacttcttc gcagggcgaa ccggccgctt
     1501 cgatccgacg gtttttctct tcgatgctca cgcgggcggt gtgggcctgg cgccgcgcat
     1561 ttacgagcgc gccgaagagc tgctcggacg cgttcggact ctgctcgcgg gttgcccgtg
     1621 tgaggcggga tgccccgcgt gtgtggggcc gtcggacccg agcggagtcc gaaagcgcct
     1681 cgcgttgcgg ctactggctg ccttggctgc ggcgcccctt cgcgccgagg ccgtttagcg
     1741 ccaaaagacg gtcgcgaatt cgccgctgaa ttcaacgaag ctgcggcgcg gtgtcgaaaa
     1801 gttcagtcgc attggcgaat cgggaaggac gttgcaaaaa cgccaatctt gtccgaaatc
     1861 actcgggacg gtgcgtgcct cgaagaggaa cggcgtgcta ccgttcgagt cccaagatgg
     1921 tgcgagcggc agggttcttc tcggtggcag cgctgttcgc gctcgggtgc cgaggcacgg
     1981 gacctcacat caaccgggtg gtcgacaacg aggatgcagg cacgggcatc gacctcaccc
     2041 cggacgcggg cgccaaagac gacgcttcga gcccgcaagc cgagccacac gctttgctca
     2101 gcgcggatcc cgcgcacggt ccctttgcag gcggcacgct cgtgctcttg cgcggcaacg
     2161 ggttcgcgag caacgcgcgc gtttggttcg gcgccaatgc cgtaccggcg ggtgacgcgc
     2221 tgccggtcga ctcgcatcgc attcaagtga ccgcgcccgc gggcgaacca ggtccgaccg
     2281 accttgtcgt gcagaacgga gacgacgagt ctacgcgcgt cacgctgcgc ggcggctaca
     2341 cgtacgacgg ctactacgcg gagcccaaca gcggccctac gagcggcgga acgctcgtca
     2401 ctttgcacgg ctatggcacg agctgggacg cgacgaccag tgtcacgatc gaccgcaatc
     2461 cctgcgaagt cgtgagcgtc gacgacccaa ccaccctcac ctgtcgcgcg cccgcaggga
     2521 cgccgggggc caagccaatc cgcgtgacca ccggcgacgg cgcgtccatc gacgtgctcg
     2581 atggtttcac ctacagcaac agcgacaacg gctttcgcgg gggcctctcg ggcgcgcccc
     2641 tcggcgacga gctgaaggtg ctggtgttcg gg
//
LOCUS       DASTKS010000399         8359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_732061, whole genome shotgun sequence.
ACCESSION   DASTKS010000399 DASTKS010000000
VERSION     DASTKS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732061"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..882
                     /locus_tag="VFQ35_08240"
     CDS             256..882
                     /locus_tag="VFQ35_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930662.1"
                     /translation="MPTTAEILRGLTRIANEARYVSIAWHVVAGISILLVLAGFRPRQ
                     RTSALLLSLPLFSVSALALNFGNPFNAAAFALLAFGLAVLASSGRAAVPLGPSWAVTL
                     GTLLMAFGWIYPHFVATTSWLTYLYAAPLGSIPCPTLSFVVGVALVGGGFALDAWCVT
                     LAFAACFYALFGVFRLGVAIDVVLLAGGIGLLVLRLQHRPANRVALRS"
     gene            1180..1776
                     /locus_tag="VFQ35_08245"
     CDS             1180..1776
                     /locus_tag="VFQ35_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930663.1"
                     /translation="MAVRTGLSFPFGNATGNSADTLSRRYAWQIPIAFDLGAKITRSI
                     FVGTYLQVGFGAEGSDNEVAAFCDDNDENFSNDVSCSVLTARLGLLMNYQFSPDKVLN
                     PWIGYGIGFESASQSLKDKQHGYSESTTVSGLTYAQLSGGFDFRGAVGVGPYAEIALG
                     AFSRTSTEQYGRKVYSGSIDDRALHAWVTLGVRFVVRP"
     gene            1773..2183
                     /locus_tag="VFQ35_08250"
     CDS             1773..2183
                     /locus_tag="VFQ35_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930664.1"
                     /translation="MTLPHTPWLRFATVALCLAACSSESGGPARTTVGATLETADGSS
                     PTVCVRLPALLGSQAEASIPVGGAFEISVHALRHTATITFPGARNDATATRNLSLTQL
                     TSGYSDVVSVTGADGVEYLVQVFSGCDESTANDI"
     gene            2209..3279
                     /locus_tag="VFQ35_08255"
     CDS             2209..3279
                     /locus_tag="VFQ35_08255"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HET9930665.1"
                     /translation="MLHARLFFAALVACGCAQTSSRTSSTDAHHSKTLAHALEFSGMC
                     DASAAIPLSRSLFIAVSDEDNVLRLYDADRPGPPLRELDFSAALGLVKPTRSDPLRAE
                     ESDLEAATRVGDLAFFVTSHGRSSSGKFKSARLRFFATTTPPDGAATLVGQPYERLLD
                     DLLAEPSLAAFGLRAAAELPPKARGGLNLEGMTARPEGGVLLGFRNPIPNGRALLVPL
                     ENPSELIRGNANARFGAPITLDLDGLGVRGLSHHRDRYLIEAGHFDRGGASSLFVWDG
                     PGQSPVRVQQLDLSDFNPEGFFTPENRDEILLLSDDGTRTTDGVECKRLRDPAARTFR
                     GLWLTLPRQSSAAPAKDPRMGK"
     gene            complement(3307..3696)
                     /locus_tag="VFQ35_08260"
     CDS             complement(3307..3696)
                     /locus_tag="VFQ35_08260"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HET9930666.1"
                     /translation="MKVNGSFDVKMTAEPPFDAVEGITLGRARFDKRFTGPLDATSVV
                     HMLSCLDAAKSSGAYSALERVTGSIGGKTGTFVLQHTGVMDAGRPSLVVTVVPGSGTG
                     ELIGLRGRMEIRIEQGAHFYELDFELA"
     gene            3845..4609
                     /locus_tag="VFQ35_08265"
     CDS             3845..4609
                     /locus_tag="VFQ35_08265"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9930667.1"
                     /translation="MRLLPPRSLSEYVAHFWSVRWALDEPRLAETLPHPTFHMVFESS
                     TEARGRGEVAGVPRGRFSRTLQGAGWVFGVKFRPAMFEPLLGASASTLTGRVVPIDSI
                     FGEVGSGLSLAMCDAQKFEVRAKLVENFLVPRLRQFPAASTSTNRRVRDLVERMATDR
                     SLLRVESAAELLETDPRTLQRHFRRYVGVSPKWVILRYRLHEAAERLKGPNPPSLAAL
                     AAELGYADQAHFARDFRKWVGQPPSEFASPRAPYVR"
     gene            4809..7154
                     /locus_tag="VFQ35_08270"
     CDS             4809..7154
                     /locus_tag="VFQ35_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930668.1"
                     /translation="MLRPQDEGTSSVAQSATPVTLAPGSVVTPKGEEHLVARAFEATP
                     SALGICDRSLRFERLNAAARAMLELPLDQVTEHFAFEFIPSLPRSEHLVLAEEALRSG
                     KTTCAEGVVAEPGASGVVARSVSFTPMPASDGEPDGLLVTFTGRRIESTPAHFETFFH
                     DSSYAIASTTLTGRVLSWNAGAEQLFGYSAAEMVNEQVDVLDTSDRALTELLGRVKQG
                     VVELEAELFRKDRSKVDASLVAAPVRNAAGEVATMLLVARDVSSRKALEKRVHNLSQL
                     EAVARVAAGVAHDVNNVLTIVNTYTGFVAQGPLTTEQAHDLDVARDAASRGAALVSQL
                     LGLGQNQSAELVVVELGEVARSIDEMLRRTLGRDVELTLTLPSAPLPVKASVGQIDQV
                     LLNLSLNARDAMPRGGRLSVSVRNSAVSPGHPLSAELAPGPYAVISVKDTGTGMDPET
                     LSRIFEPFFTTKRRGEGTGLGLLIVREIVKQLHGAIRVSSKLGDGTEFQVFLPVHEPR
                     PRTAPPPPFESDEARPQTVLVVEDDVTIRSALRRILHAEGYAVLEAADGLEATEVSTN
                     FGGNIDLLLCDLLMPRVDGRQTVACIRETRPDVRVVFVSGQRSAEEAIVEGAGFVQKP
                     FSRDELLIAVKAALDRPPLEPEPKLPDQPVVLLVDDSSEFRDSLARLLEESDLIALSA
                     KSGLHALQMLESQHVDMIVTDQFMPGIDGVRLLELVRHRWPHCQRVLFTAYASSDVLM
                     DAVNRGGAHRVLVKSMHPMAIRDQIEAAVLSAARFYERARR"
     gene            7439..7645
                     /locus_tag="VFQ35_08275"
     CDS             7439..7645
                     /locus_tag="VFQ35_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930669.1"
                     /translation="MKPFTDTRRIFWIGGVTLILIGLIAAFIVLPASSPLKSTSEPEV
                     RVPKMENVPEGDGSKLKLEVDKKD"
     gene            7693..8169
                     /locus_tag="VFQ35_08280"
     CDS             7693..8169
                     /locus_tag="VFQ35_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9930670.1"
                     /translation="MMLTYRSTAFALLLAAPLLFATPAAADVAPVNSCNTEGSDCTNG
                     LPEGANPWGPGPFAPGICTKKTCSRGSPDGGVVHYDCLLCVATPGQGGSPGTGGSTAA
                     GGNGTGGAPTAGKSGSSGGSDGCSCRVPGGAKPWTLAGAMLVVGIVALAGGRRRTR"
     gene            8166..>8359
                     /locus_tag="VFQ35_08285"
     CDS             8166..>8359
                     /locus_tag="VFQ35_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930671.1"
                     /translation="MNSLAVVASLERAAWPLDRLRTRFFAWALRWRALAPLLLQRDRR
                     LAVTLTARAAFAFALAVYAPV"
BASE COUNT         1405 a         2797 c         2621 g         1536 t
ORIGIN      
        1 gtccggatcc gccagcgcca acgcccatgc cttcatcgct cccttcgcac gcaccgaaaa
       61 cgatcgcaga cgagaagacg agggcagtac cggtagaccg cagcaacgac atggtgcctt
      121 cgccagcaat ttggatgccg actccttcgt cgcggtctct cggggtgaat cgcctgacag
      181 tcgccgcact gacacagact ggcaagcatc accgccgcat tcgctgcggg cacgacgctt
      241 gcgaccacca cgaccatgcc tacgaccgct gagatcttac gcgggctcac ccgaatcgcg
      301 aacgaagcgc gctacgtcag catcgcttgg cacgtcgtag cgggaatttc gattctcttg
      361 gttttggcag gcttcaggcc gcgacagcgg acgagcgctt tgctgctctc gttaccgttg
      421 ttctcggtga gcgcgctcgc gttgaacttc ggtaacccgt tcaacgccgc agcttttgcg
      481 ttgctcgcgt tcgggcttgc agtcttggcg tcgtcgggtc gcgcagcggt gccactcggg
      541 ccgagctggg ccgtcacgtt ggggacgctg ctcatggcgt tcggctggat ctatccgcac
      601 ttcgtcgcga ccacgagctg gctcacgtac ttgtacgcag cgccgctcgg ctcgattccc
      661 tgcccgaccc tatcgttcgt ggttggcgtc gcactcgtgg gtggagggtt cgctctcgat
      721 gcctggtgtg tcacgctggc gttcgcagcc tgcttctacg ctctgttcgg cgtattccgc
      781 ttgggcgtcg cgatcgacgt cgtgttgctc gcaggcggaa ttgggctact cgtgctcagg
      841 cttcagcacc gccccgcgaa ccgcgtcgcg ctgcgttcgt agcgaattaa gaaacccgcg
      901 cggcgcgtgc ctcggcaacg gctgtccggg aattccgcat tcggcctccg ttgtcatctc
      961 gtacaaacct ccaagaggca tgatgcgcac ccgtggccgc gcgcaactac attgatcgaa
     1021 atcatcgcct catgaaatcg tcattcgatg cgcctcgcgt cgtttgcgct aagagcctgt
     1081 gtcgtctcag ccgtcgctgc gctctccgga accgcacgcg ctgcgcccac tcccgagagc
     1141 gagttcgccg gcgcgtcgcc cgcgagcacg ggctttcaaa tggcggtgcg aaccgggctc
     1201 tctttcccgt tcggcaacgc aacgggcaat tctgcggata cgctgagccg ccgctacgct
     1261 tggcagatcc cgatcgcctt cgacctcggc gcgaaaatca cccgctcgat tttcgtcggc
     1321 acttatctgc aggtcggctt cggcgccgaa ggcagcgaca acgaggtcgc cgcgttctgc
     1381 gacgacaacg acgagaactt cagcaacgac gtgagctgca gcgtcctcac cgcccggctc
     1441 ggcctgctca tgaactacca attttcgccc gacaaggtcc tgaacccgtg gatcggctac
     1501 ggcatcggct tcgagtcggc gagccagtct ctcaaggaca aacaacacgg ctatagcgag
     1561 tccacgacgg tctccggcct cacttacgca cagctttcgg gcgggttcga cttccgcggc
     1621 gcggtcggag tagggccgta cgcagagatt gcgctgggcg cattctcgcg aaccagcacc
     1681 gagcaatacg gtagaaaagt ctactcaggt tcgattgacg atcgcgctct tcacgcgtgg
     1741 gtcacgctcg gggtaaggtt cgtggtgcgc ccatgacgtt gccgcacacg ccctggctcc
     1801 gcttcgcgac ggtagccctg tgccttgccg cctgttcttc cgagagcggc ggcccggctc
     1861 gcactaccgt gggtgccacg ctggaaacgg cggacggctc gtcgccgacg gtatgcgtgc
     1921 ggttgccggc gctgctcggc agccaggccg aagcgagcat accggttggc ggcgctttcg
     1981 agatcagtgt ccacgctctc cgccacaccg ctaccatcac gttcccaggg gcgcgcaacg
     2041 acgccacagc aacgcgaaac ctgtcgctca cgcagctcac gagcggctac tccgacgtcg
     2101 tgagcgtcac cggcgctgac ggggtggaat atctcgtcca ggttttctct ggctgcgacg
     2161 agtccacggc gaacgacatc tgagcgcacc ggctctaaac tgagccgcgt gctccacgct
     2221 cgactcttct tcgccgcgct cgtcgcgtgt ggctgtgcgc agacttcctc gcgaacgagc
     2281 tcgaccgacg cacaccactc gaagacgttg gcccacgccc tcgagttcag cggaatgtgc
     2341 gacgcttccg ctgcgattcc gctctcgcgt tcgctcttca tcgccgtaag tgacgaagac
     2401 aacgtgctgc gtctctacga cgccgaccga ccggggccgc cgctgcgcga gctggacttt
     2461 tcggcggcgc tcggcctcgt gaagcccact cgcagcgatc ccctgcgcgc cgaggaaagc
     2521 gatctcgagg ccgcgacgcg cgtcggcgat ctcgcgtttt tcgtgacctc ccacggaaga
     2581 agtagctccg gcaagttcaa gtcggcgcgg cttcgcttct tcgcgaccac cactcccccg
     2641 gacggcgccg caacgctggt cggtcagccc tacgaacgcc tgctcgatga tctcctcgcc
     2701 gagccaagcc tcgccgcttt cggcttgcgc gccgccgccg agctaccacc gaaggcgcgc
     2761 ggtgggctca acctcgaagg gatgacggcg cgccctgaag gcggcgtctt gctcggcttc
     2821 cgcaacccga tcccgaacgg ccgagcgctg ctcgttccac tcgagaatcc cagcgagctc
     2881 attcgcggca acgcgaacgc tcgcttcggc gcgccgatca ctctcgacct cgacggcctc
     2941 ggcgtccgcg ggctctcgca ccaccgtgac cgctacctca tcgaggcagg tcatttcgat
     3001 cgcggcggag catctagcct gttcgtgtgg gacggccctg gccaatcgcc cgttcgcgtc
     3061 cagcaactcg acctctccga cttcaaccca gagggcttct tcacgcccga aaatcgggat
     3121 gagatccttc tcctgagcga cgacggcacc cgcaccaccg acggcgtaga gtgcaagcgc
     3181 ctgcgcgatc ccgccgcaag aaccttccgt ggcctctggc ttaccttgcc tcgtcagtcg
     3241 agcgccgcgc cggctaagga tccgcgaatg ggtaagtgat actgcgcgag ccgatccact
     3301 aagggcttac gccagctcga aatcgagttc gtaaaagtga gcgccttgct cgatgcgaat
     3361 ctccatgcgt ccgcggaggc cgatgagctc gccggtgcca gagcctggaa ctacggtcac
     3421 cacgagggaa ggccgccctg catccatgac tcccgtgtgc tgcaggacga acgtgcccgt
     3481 ctttcccccg atgctgccgg tcacccgctc gagggcactg tatgcgccag aactcttcgc
     3541 ggcgtcgagg cagctcaaca tgtgcacgac ggaggtcgcg tccagcggac cggtgaagcg
     3601 cttgtcgaac cgggctcgcc cgagcgtgat gccctcgacg gcgtcgaagg gcggctcggc
     3661 ggtcatcttc acgtcgaagc ttccgttcac tttcatgcgc ttcttctacg gaatgcggga
     3721 gcctcggtct tgaaaaaacg cgacaacagg gccacgttca agcgtgccga gcgacccacg
     3781 gaaactcgct ccgcccccgc gcggggtcct cagcgcgagc tccggacccg cgagtaccga
     3841 gcacgtgcgg ctcctgccac cgcgctcgct gtcggaatac gtcgcgcact tctggagcgt
     3901 ccgttgggcc ctggacgagc cgcgcctcgc cgaaacgctg ccacacccaa cctttcatat
     3961 ggtcttcgag tcatccaccg aagcgcgcgg tcgcggagag gtggcgggag taccgcgtgg
     4021 tcgcttctcg cgcaccctcc agggtgcagg ctgggttttc ggagtgaaat tcagacccgc
     4081 tatgttcgag ccgctcttgg gcgcgtcggc gagcacgttg acgggccggg tggtcccaat
     4141 cgattcgatt ttcggagaag tggggtcggg cctttcactc gcaatgtgcg atgcgcaaaa
     4201 attcgaagtt cgagcgaagt tggtcgaaaa cttcttggtg cccaggctcc ggcagttccc
     4261 ggcagccagc acgtcgacga accggcgcgt gcgggacctg gtcgagagaa tggcgaccga
     4321 ccgctcattg ctgcgcgtcg agtcagcagc cgagttgctc gagactgacc cgcgcactct
     4381 gcaaaggcat ttccgccgct acgtgggcgt gagcccgaag tgggtaattc tgcgctatcg
     4441 cttgcacgag gccgcagagc gtctgaaagg cccgaacccg ccgtcgctcg ccgcgctcgc
     4501 cgccgagctc ggctacgcgg atcaggccca ttttgcgcgt gattttcgca agtgggtggg
     4561 gcaaccgccg agcgaattcg cctccccgcg cgcgccctat gtgcgctgat gtgtcagcgc
     4621 agatcactcc cgcgagtgat cttttagcct gcggatgcgt gtcaagaatg agttcgataa
     4681 gtgcggctcg tgcacagttg caaactggga ggactggatt ctgcgtcaac gacttgacgg
     4741 cgccacggag gagagagcat cgtaagtcgc gcgcttgggt tccgcgcgca aaagaaaagg
     4801 attctcgggt gctgcgacca caggatgaag gcacgtccag cgttgctcaa tccgcgacgc
     4861 cagtgacgct ggcgccagga tcggtcgtca cacctaaggg tgaagagcac ctcgtcgcgc
     4921 gcgctttcga agcgacgccg agcgcgctcg gcatttgcga tcgctcgctg cgcttcgagc
     4981 gcttgaacgc cgctgctcgc gccatgctcg agttgccgct cgatcaagtg accgagcact
     5041 tcgccttcga gttcattccg agccttccgc ggagcgagca cctggtgctg gcggaggagg
     5101 ccctgcgcag cggcaaaacc acgtgtgcag agggagtggt cgccgaaccg ggcgcgagcg
     5161 gcgtcgtcgc gcggtcggtc agcttcacgc cgatgccagc gagcgacggt gagcccgatg
     5221 ggctgctcgt gacgttcacg ggtcgccgca tcgagtccac acccgcgcat ttcgaaacgt
     5281 tctttcacga cagcagctac gcgatcgcct cgacgaccct cactgggcgc gtgctcagct
     5341 ggaacgccgg cgccgagcag ctcttcggct acagcgcggc tgagatggtg aacgagcaag
     5401 tcgacgttct cgacacctct gaccgcgcgc tcaccgagct gctcgggcga gtgaagcaag
     5461 gggtcgtcga gctcgaggcc gagctctttc ggaaagatcg cagcaaagtc gacgcctcgc
     5521 tggtcgcagc cccggtgcga aacgcggccg gagaggtcgc caccatgctg ctcgtcgcgc
     5581 gcgatgtctc gtcgcgaaaa gcgctggaaa agcgcgtcca caacctgagt cagctcgagg
     5641 cggtggcccg ggtcgcggcc ggtgtcgctc acgacgtgaa caacgtgctc acgatcgtaa
     5701 atacctacac tggcttcgtc gcccaggggc cgctcacgac ggagcaagcg cacgacctcg
     5761 acgtagcgcg cgacgcggcc agtcggggcg cggcgctcgt gagtcagctg ctcggcctcg
     5821 gccaaaacca gagcgcggag ctcgtggtcg tcgagctcgg tgaggtggcg cgcagcatcg
     5881 acgagatgtt gcgccgcacg ctcggccggg acgtggagct cacgctcacc cttccctcgg
     5941 caccgctccc ggtcaaagcc agcgtcgggc agatcgatca ggtgctgctc aacctttcgc
     6001 tcaatgcgcg cgacgcgatg ccgcgcggcg ggcgcctctc ggtcagcgtg cgtaactccg
     6061 ccgtgagccc gggacatccg ttgagcgccg agctcgcgcc ggggccatac gccgtcatca
     6121 gcgtcaagga caccggcacc ggcatggacc cggagacgct ctcgcgcatc ttcgagccgt
     6181 tcttcacgac caagcgacgc ggcgagggta cgggcctcgg cttgctcatc gttcgtgaaa
     6241 tcgtcaagca gctgcacggg gcgattcgcg tcagcagtaa gctcggcgac ggcaccgagt
     6301 ttcaggtctt tctgccggtt cacgagccac gaccgagaac ggctccccca ccgccgttcg
     6361 agtcggacga ggcgcgacct caaacggtgc tcgtcgtgga ggacgacgtc accatccgca
     6421 gcgctctccg tcgcatcctg cacgcggagg gatacgccgt gctcgaggcc gcagacgggc
     6481 tcgaagcaac ggaggtgtcg acgaacttcg ggggcaacat cgatttgcta ctttgtgatt
     6541 tactgatgcc gcgcgtcgat ggtcgccaaa ccgtcgcctg catccgcgag actcgaccgg
     6601 acgtgcgggt cgtgttcgtg tccggtcagc gcagcgccga agaagcgatc gtcgagggcg
     6661 ccggcttcgt gcaaaagccc ttttcgcgcg acgagcttct catcgccgta aaggcagcgc
     6721 tcgatcggcc gccgctcgag cccgagccca agctacccga tcagcccgtg gttctcctag
     6781 tggacgacag cagcgaattt cgcgactcct tggctcgcct gttggaggag tccgacctga
     6841 tcgcgctctc ggccaagagc ggcctgcacg ccttgcaaat gctcgagagc cagcacgtcg
     6901 acatgatcgt caccgaccaa ttcatgcctg gcatcgacgg ggtccggctg ctcgagctcg
     6961 ttcggcaccg ttggcctcac tgtcagcgtg tactctttac cgcctacgct tcgtccgacg
     7021 tattgatgga tgcggtgaat cgtggcggcg cccaccgcgt gctcgtcaag agcatgcatc
     7081 ccatggcgat tcgagaccaa atcgaagcgg ccgtgctcag tgcggctagg ttctacgagc
     7141 gagctcgtcg ctgagaccga ctccgcaatt ccggatcgaa ccgcgctgac gagcccaaag
     7201 ccgcccggcc cgagtcgagt cggcatcgaa cgcaattgct gcgctgaaca tcgacggtcg
     7261 cagggactgc gccgtagtcg tgattcggcg ctcccgctta cggttttggc catgtgatat
     7321 cggccttcat tcgaggctcc tcgaggctcc gcgatcgtaa aacgagcgct tttcagggct
     7381 gcgaatgacg gctcgacccc ggtcgcacat tggcgcactc cttgctcggc gcttggtcat
     7441 gaaaccattc accgacaccc gcaggatctt ctggatcggc ggggtaaccc tcatactaat
     7501 cgggctcatc gccgcgttca tcgtattgcc cgcgtcgagt ccgctgaaga gcacctccga
     7561 gcccgaagtt cgagtaccga agatggagaa cgtgcccgag ggagatggtt ccaagttgaa
     7621 gctcgaggtc gacaagaagg attgatcgag ctcgagtctg tctttgccct gctttgcgca
     7681 atgaaaggaa cgatgatgct gacctatcgc tcgaccgcgt tcgctctgct tctggccgct
     7741 ccgctcctct tcgcgacgcc cgctgcggcc gacgtcgcgc ccgtgaatag ctgcaacacc
     7801 gaaggctcgg attgcaccaa cgggctgccc gaaggtgcga atccgtgggg accgggcccg
     7861 ttcgcacccg gcatttgcac gaagaagacg tgcagccgtg gctcacccga tggtggagtc
     7921 gttcattatg actgcctctt gtgcgtcgcg acgccgggac agggaggctc gcccggtacg
     7981 ggaggcagca ccgccgcggg tggcaacggg actggaggcg cgccaacagc gggcaaaagt
     8041 ggctcttctg gtggctcgga cggctgttcg tgccgcgtgc ccggcggagc caagccttgg
     8101 acgctcgcgg gcgcgatgct cgtcgttggc atcgtagcgc tagccggcgg gcgtcgtcgc
     8161 acgcgatgaa ctcgctggcc gtcgtagcgt cgctcgaaag agccgcgtgg ccactcgatc
     8221 gccttcgcac tcggttcttc gcctgggcgc tccggtggcg ggcgctcgcg ccgctcctcc
     8281 tgcaacgcga ccggcgcctg gccgtaacgt tgacggcgcg cgccgcgttc gctttcgcgc
     8341 tcgccgtcta tgcgccggt
//
LOCUS       DASTKS010000400         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1848604, whole genome shotgun sequence.
ACCESSION   DASTKS010000400 DASTKS010000000
VERSION     DASTKS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848604"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VFQ35_08290"
     CDS             <1..1470
                     /locus_tag="VFQ35_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HET9930672.1"
                     /translation="GTLVIKSSRARGADQPDAEFSRSIAEEVMESHKPVVALNARNDA
                     RMANYRSVHQLLVESVACVPVLAPHGRAIGALYVETRARKGSHFERELPTLRAFADQV
                     AIAVENTRLIEENLKRTGDLERANQQLSEAQERLRELLGDRTEQLKRARRRLKDARET
                     LYGHFGYHGLVGTSTAMRRVYALIERVKDTDVPVLITGESGTGKEMVARAIHETSPRA
                     KAKMLGVNCGAIPENLLESELFGHVRGAFTGADRERRGLFRECDGGTILLDEIGETPL
                     KMQAGLLRVLQERTVRPVGGSQEQVVDVRVICATNRDLQTMVREGKFREDLYYRIHVV
                     EVRLPALRERAEDIPQLVDHFLGIFSARYKREKKAVSRDALRMLADHDWPGNVRQLEN
                     VLLNAWVMSEEDELVPEDFDLPELTGARPAPAAAEAGPTPGQRRAGPKGTTSEHFRGE
                     RERILEALRACNWNRVKAAELTGIPRRTFYRRLREYGIQ"
     gene            1735..>2305
                     /locus_tag="VFQ35_08295"
     CDS             1735..>2305
                     /locus_tag="VFQ35_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930673.1"
                     /translation="MRNRSASHRARIELTLTVCGLLTSTAACSIIVNTSVPDDGSGGR
                     GVNAGGSSFGMTQSGGRRSGGGGVAGAAGSGSSLSSGGLSAAGGKSVTGAPDPTGGAG
                     ATGGVGATGGVSTPSTGGANGGGGASGSGGLNSAGGALGSGGTPASGGTAAAGGSSGG
                     VAPTGGTTQSAGSGGIGKGGSTALGGSSSG"
BASE COUNT          416 a          734 c          798 g          357 t
ORIGIN      
        1 ggtacgctgg tcatcaagag ctcgcgcgcg cgcggcgccg accaacccga cgccgaattc
       61 tcgcgctcca tcgccgaaga agtgatggag agccacaagc cagtcgtcgc cctcaatgcg
      121 cgcaacgacg cgcgcatggc aaactatcgc tccgtgcacc agctcttggt cgagtcggtc
      181 gcgtgcgtgc cggtgctcgc gccgcacgga cgcgcaatcg gcgcgctcta cgtggagacg
      241 cgcgcgcgca aaggctccca cttcgagcgg gagctgccca cgctgcgtgc gttcgccgat
      301 caggtcgcga tcgcggtcga gaatacgcgc ctcatcgaag aaaacctcaa gcgcacgggg
      361 gacctcgagc gcgccaacca acaactctcg gaagctcaag agcggctgcg cgagctcctc
      421 ggcgatcgca ccgagcaact gaagcgagcg cggcggcgtt tgaaagacgc ccgcgagact
      481 ctgtacggcc attttggcta tcacggcttg gtcggcacga gcacggccat gcgccgcgtc
      541 tacgcgctca tcgagcgcgt caaagacacc gacgtaccgg tcttgatcac gggcgagagc
      601 ggcaccggta aagaaatggt cgcgcgcgcc atccacgaga cgtccccgcg cgccaaagcg
      661 aaaatgctcg gtgtaaactg cggagcgatc ccggagaacc tactcgagag cgagctcttc
      721 ggtcacgtgc gcggtgcgtt cacgggcgct gaccgcgaac ggcgcgggct cttccgcgag
      781 tgcgacggcg gcaccatcct gctcgatgaa atcggcgaga cgccgctcaa gatgcaagcg
      841 ggcctactgc gcgtccttca agaacgaacg gtgcgcccgg tcggcggcag ccaagagcag
      901 gtggtcgacg tgcgcgtcat ctgcgcgacg aatcgcgacc ttcagacgat ggtgcgcgaa
      961 ggtaagtttc gcgaagacct ctactatcgc attcacgtcg tcgaggtgcg cttgccggcg
     1021 ctgcgcgagc gcgccgagga catcccgcag ctcgtcgatc acttcctcgg tatcttttcg
     1081 gcgcggtaca agcgggaaaa gaaggcggtt tcgcgcgacg cactgcgaat gctcgccgac
     1141 catgactggc caggcaacgt gcgccagctc gagaacgtgc tcctgaacgc ttgggtcatg
     1201 agcgaagaag acgagctcgt gcccgaagac ttcgacttgc cggaactcac cggcgcgcgc
     1261 cccgcccccg ctgctgccga agccgggcca acgccgggtc aacggcgcgc tgggcccaag
     1321 ggcaccacct ccgagcattt ccgcggcgaa cgcgagcgca tcctcgaggc gttgcgcgct
     1381 tgcaattgga accgggtcaa agcggccgag ctcaccggta ttccccggcg cactttctat
     1441 cgccggctgc gcgaatacgg cattcagtaa cgcggccaca ccgcgcgcga gtgctgcaac
     1501 ccccggttgc cgcggcaact cttggttgct ttcgatacgc gatttgaggc ggtttcggcg
     1561 gccgctgcga cgcctgccgc aacgtcgtga tgagcgaatg ctcccaaggc cctcgactca
     1621 cggcgtggta tgccctcggc atagtgcctg cacgggacga agtgagccat cggacaggcg
     1681 gtcttcgtcc actccagatc gggcgaattt ttgattcatt cacctcgggg catcatgcga
     1741 aatcgatcag cgagccacag ggcgcgaatc gaactcactc tgacggtctg cggactcctg
     1801 acgtctacgg cggcctgcag catcatcgtg aacaccagcg ttccggatga cggctcgggt
     1861 gggcgcgggg tcaatgcagg tggttcatct ttcgggatga cgcagtcggg tgggcgtcgc
     1921 agcggcggcg gcggcgtagc tggagcggca ggaagtggta gttcgctgag ttccggcggt
     1981 cttagcgccg cgggaggcaa gagcgtgacg ggagctcccg acccgacggg aggagccggt
     2041 gcgacgggcg gagttggcgc tacgggcgga gtcagcacgc cgagcactgg gggtgccaac
     2101 ggagggggtg gcgcgtcggg gtcaggcggt ctcaactctg cgggtggcgc gctcggctcg
     2161 ggcggcactc ccgcttcggg cggaacggcg gcggcaggcg gttcgagcgg aggcgtggcg
     2221 ccaaccggcg gaacgactca aagcgctggg agtggcggga tcggcaaggg cggttcaacg
     2281 gcgctcggtg gaagctcgag cgggg
//
LOCUS       DASTKS010000401         8148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1402882, whole genome shotgun sequence.
ACCESSION   DASTKS010000401 DASTKS010000000
VERSION     DASTKS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402882"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFQ35_08300"
     CDS             <1..190
                     /locus_tag="VFQ35_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930674.1"
                     /translation="GAARCFERWSVRSNGTRAMLPGAGRAEQRNAPRSERAALGTRRA
                     RNARGADRPLRGTVVLSV"
     gene            1114..3909
                     /locus_tag="VFQ35_08305"
     CDS             1114..3909
                     /locus_tag="VFQ35_08305"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET9930675.1"
                     /translation="MSELPKAYEPLEVEPRWYNYWAERGLFRASVEPNDTRPAYTIAM
                     PPPNVTGSLHMGHALYTIQDVLVRHKRMQGYNVLWQPGIDHAGIAAQIVVERQLKREG
                     KSRHDLGREAFVERVWRWKEQSGGRIAEQMRVLGYSADWERSKFTMDPDMSRAVREAF
                     VRLYEEGLIYRATRLINWCSDCRTALSDLEVENEETNGELYEFAYEVADADAGAGATE
                     LVVATTRPETMLGDTAVAVHPDDPRYTHLHGKKLKHPFVNRLVPVITDAELVDMAFGT
                     GAVKVTPAHDFNDFATGKRHQLEEINILNLDGTLNAQGGEFQGLTVKQARSAVKKALS
                     ERGLHRGEKAHTLMMPRCSRCNTVVEPMISTQWFVKTAPLAEPALAAVRDGRTQILPE
                     EWVKTYEHWLVNIQDWCISRQLWWGHQVPAYYCDDNHVTVSREAPHACGTCGKEKLRQ
                     DADVLDTWFSSGLWPFSTLGWPDKTVALDRFYPSSDMETGYDILFFWVTRMMMMGLKL
                     VGEVPFRRILLHGLVVDETGDKMGKLKGNTIDPLDLIHGADFSTVVKKAMPDAPEAEA
                     LAKFRKAYPSTAQMGKGFAACGADALRFSLCSYSPQAKRIALSPARIEGYRKFCNKIY
                     NATRFALPYLEGVTATGKAPEGPKLLINRWILSRLGAAVQASTKGIEEFRLDDGSGAL
                     YHFFWDELCDWYIEMVKSVFQSGSAEEQAETRATLSHVLESSLRALHPFMPFITEELW
                     QRLPRPAGAKDSIMVAAYPTLADGPYSAEAERDMNVLMAAVSAARSTRSEHDLPRQAK
                     VPLELRAADPALRALLERESRYVELLVGTDGAPKVAAPSPERPRGFLVSTAGDVEVLV
                     GLRGLVEAKKEEERLSRQLKKTEKDIKVLEGRLANKNFVDNAPPEVVAESKALLEQMK
                     RQFQRIQEAQALVHELE"
     gene            complement(3990..4739)
                     /locus_tag="VFQ35_08310"
     CDS             complement(3990..4739)
                     /locus_tag="VFQ35_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930676.1"
                     /translation="MQLARTLGPLAFIAVSLSTIRASAQDYTHRSFLTTDGHFEITGE
                     PSRPQIVRIDVSNHSPHPIWLAPHFYWGVSDHVTIGITHRYGLCVTGCERAYNDVGFG
                     LLVGLVQKHGFELDINTGIQSRSLDPFYVGWKGGVIGRISGDRVAFVFDPSVYVGFNR
                     RDRGNGDELVLPFWVYIQATRQVVPFVGAMLVGPVRGFGDNLSVPVEGGVLFRIGERV
                     DLGAYFRFYNLLGRGGNPDGREAGMLGRFRF"
     gene            5000..6004
                     /locus_tag="VFQ35_08315"
     CDS             5000..6004
                     /locus_tag="VFQ35_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9930677.1"
                     /translation="MPVEREESTGVGVSRSEAAALAVLDEPTDLVPAFEHDDDESTAL
                     VSLESLGRIEHHEPKDRHLLVRVKGAGLGQVTRLGDKPCYVGRGSEAGLYVSDSGVSR
                     KHAMLIPDGETYVLIDTESANGTFVGGQRVSRHVLRDGDVVQFASTASFRYTLTDASQ
                     EALLRQLFEASVTDALTGAFNRDHFDTQLAAELSYARRHKTELSLVMFDVDFFKKVND
                     TYGHPSGDQVLVRIARTVAGMVRTEDVFARYGGEEFALILRGVNVAGAAALAERLRGA
                     VQALTIPSDRGPIRVTISAGCAAVSGGAEITAEELVGAADKRLYAAKHGGRNRVHSAD
                     "
     gene            6057..>8148
                     /locus_tag="VFQ35_08320"
     CDS             6057..>8148
                     /locus_tag="VFQ35_08320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9930678.1"
                     /translation="MPPGQELDSEPRARLPSLLGAIVAGLLLIALMSREGHPGLSVPL
                     GLGLVLAASFGLLYGLGFFTKSEVVGRSVPVRELAAPFATTLASVVALVVALRLAVAG
                     GLPMPLLSSAVLVTSTSLACVFSLGWCCDVARIDPEAPPFWRRHGVWLLAIAIVAYVP
                     MLGNYSLYDPWETHYGEVAREMLAREDWISFWWAQENWFWSKPALDFWLQGLSFLLLG
                     VRYRPDEMLAGVADGLNPAPEWAARAPVILLTLVANYLLYKAVARFLGRRVGFVAGLA
                     LTLAPYWYLIAHQSMTDMPYAAPLTAALALLLIGLDLSPRATVASIGVRVGRRELWFG
                     KHHAVLLLVVASAIPQIAYLLSRHVSWSLAGASHFLRVHADQFMAGSGGDNCGLPGND
                     PCEASAAVLPELPPGLMALLWIAALAAFLWLNREESRAQRWAFLAGWYFVAVSALGQG
                     APGLVLPVVVVVTALFALRRLRDLERLEWGGFLLIVACVTLPWYVQAFARHGTGFTDR
                     LLFYDMYKRAFVHVHDTNAGDDVSFRYYIWQLGYGLFPFTGVALWGFLKSFAVRPKAE
                     AKTRDVLVFLGIWSLLGFAMFSVSLTKFHHYALPVAPPAAVLAGVALHEFVGDARTEN
                     VRQAVVRYGAAAAAAALALAATVWLTQGSWFGVRTEPNVLRAALGSPAFIAAVACFAV
                     SVRFGGPLAASAPVN"
BASE COUNT         1397 a         2512 c         2871 g         1368 t
ORIGIN      
        1 gggcgccgcg cgatgcttcg agcgctggag cgtgcggagc aacggaacgc gcgcgatgct
       61 tccgggcgct gggcgcgcgg agcaacggaa cgcgccgcgc tcggaacgcg ccgcgctcgg
      121 aacgcgccgc gctcggaacg cgcgaggagc ggatcgtccg ttgcgaggaa ccgtcgtgtt
      181 gtcggtgtga ggagccgcct gggtcgcttg gcgcgcatga gaccgcagag tgcagtgccg
      241 actgcacacg atgatcctct tgcgagtgac cgctcacaac gcgcgcgttc gagcgagcgc
      301 cctcgcgaac taccgcacgc agccacgcag agccggcatc cgagccacgc gcggggcaaa
      361 aagcagcgta agaagaggcg agcagtaatt cagcggccag cgaacacagc aaaccggcga
      421 tgctgcccca gcaaccccgc aagccaccga agcaccgaag ccgcaacgcg gtcccagcaa
      481 gcgagtcagc gaagcagcag cgcggcccaa gtaagcgagt cagcgcatca gcagcgcaac
      541 acggcagcga atcagcgaag cagcagcgcg gcccaagcaa gcgagtcagc gaagcagcag
      601 cgcaacggat ccgctcaatc atctgtgcgc ggagtgcgca acacggcagc gagtcagcga
      661 agcagcagcg cgacacggca gcgagtcagc gaagcagcct cccagcgagc gaaagtgtgc
      721 acaacgcaca agctttcgga gggagtcggg agcgggatcg ggagcgcgcg ggaagggacc
      781 ccgcacgagg aggcgttgga ctcgccgtaa cgcatcaggt agtggcagca cagcgaagcg
      841 ctcccacggg ggagggcccc gcgcggttcc gatcccgctc ccggcgaccg atcccgtacc
      901 cggcgaccga tcgtttctgt gggagccaga gagggcttgc cgtaagggtg cggcgcgggc
      961 gcggtgggag tgccgtgtgg cgggcagcag ggtctgcgct atggccgaaa agggcccgtc
     1021 tacgcgcact tttcagtggg agcgccctcc tgagacaggg accggaaaat cgcgaatgca
     1081 tttgcgggcc gaggtactag ctttgctcgc cccatgagcg agttaccgaa agcctacgag
     1141 cccctggaag tcgagccgcg ctggtacaat tattgggccg agcgcgggct gtttcgcgcg
     1201 agcgtcgagc cgaacgatac gcgaccggcg tacacgatcg cgatgccgcc gccgaacgtg
     1261 acggggtcgc tgcacatggg tcacgcgctc tacacgattc aagacgtgct ggtgcggcac
     1321 aagcggatgc agggctacaa cgtgctgtgg cagccgggga tcgatcacgc cggcatcgcg
     1381 gcgcagatcg tggtggagcg gcagctgaag cgcgaaggga agtcgcgcca tgatttggga
     1441 cgcgaagcgt tcgtggagcg ggtgtggcgt tggaaggagc agagcggcgg gcgcatcgcg
     1501 gagcagatgc gcgtgctggg ttactcggcg gattgggagc gcagcaagtt cacgatggat
     1561 ccggacatgt cgcgcgcggt gcgcgaggcg ttcgtccggt tgtacgaaga ggggctgatt
     1621 tatcgggcga cgcggctcat caattggtgc tccgattgtc ggaccgcgct cagcgatctc
     1681 gaagtggaga acgaggagac gaacggcgag ctctacgagt ttgcttacga ggtggccgac
     1741 gcagacgccg gcgcgggggc gacggagctc gtggtggcga cgacgcggcc ggagacgatg
     1801 ctgggtgata cggccgtcgc ggtgcacccg gatgacccgc gctacacgca ccttcacggg
     1861 aagaagttga agcatccgtt cgtgaatcgg ttggttccgg tgatcacgga cgcggagctc
     1921 gtggacatgg cgttcgggac gggcgcggtg aaggtgacgc cggcgcacga tttcaacgat
     1981 tttgcgacgg gcaagcgcca ccagctcgaa gagatcaaca tcctgaacct ggacgggacg
     2041 ctgaacgcgc agggcggcga gttccagggg ctgaccgtga agcaggcgcg gagcgcggtg
     2101 aagaaggcgc tttcggagcg cgggctgcac cgcggggaga aggcgcacac gttgatgatg
     2161 ccgcgttgca gtcgttgcaa cacggtcgtc gagccgatga tttcgacgca gtggttcgtg
     2221 aagacggcac cgctcgccga gccggcgctc gcggccgtgc gcgacggacg cacgcagatt
     2281 ctgcccgaag agtgggtgaa gacctacgag cactggctcg tgaacattca agactggtgc
     2341 atctcgcggc agctgtggtg ggggcaccag gttccggcgt actactgtga cgacaatcac
     2401 gtgacggtga gccgggaggc gccgcacgcg tgcgggacgt gtggaaaaga gaagctgcga
     2461 caggacgccg acgtgctcga tacgtggttc tcgagcgggc tttggccgtt ttcgacgctg
     2521 ggttggcccg acaagaccgt ggcgctcgat aggttctatc cgtcgagcga catggagacg
     2581 ggctacgaca ttttgttctt ctgggtgacg cgcatgatga tgatgggctt gaagctcgtc
     2641 ggagaggtgc cgtttcgcag gattttgctg cacgggctcg tggtggacga gacgggcgac
     2701 aagatgggca agctgaaggg caacaccatc gatccgctcg atttgatcca cggcgcggac
     2761 ttcagcaccg tcgtgaagaa ggcgatgccg gacgcgccgg aggccgaagc gctcgcgaag
     2821 ttccgaaagg cgtatccgtc gaccgcacag atgggtaaag gcttcgctgc gtgcggagca
     2881 gacgcgctgc gcttcagctt gtgcagctat tcgccgcagg cgaagcgcat cgcgctgtcg
     2941 ccggcgcgca tcgagggtta ccgcaagttt tgcaacaaga tctacaacgc gacgcgcttt
     3001 gcgttgccat acctcgaggg cgtgaccgcg accggcaagg cgccggaagg gccgaagctg
     3061 ctcatcaatc gttggatctt gtcgcgtctc ggcgccgcgg tgcaggcgag cacgaagggc
     3121 atcgaggagt tccgcctcga cgacgggtcc ggcgcgctct atcacttctt ctgggacgag
     3181 ctctgcgact ggtacatcga gatggtcaaa tcggtgttcc agagcggcag cgccgaggag
     3241 caggcggaga cgcgcgcgac gttgagccac gtgctcgagt cgtcgctccg cgcgttgcat
     3301 ccgttcatgc cgttcatcac cgaggagctg tggcagcgct tgccgcgtcc ggcgggcgcc
     3361 aaagactcga tcatggtcgc ggcgtatccg accctcgcag atgggccata ctccgcggag
     3421 gcggagcgcg acatgaacgt gctgatggcg gcggtgtcgg cggcgcgcag cacgcgcagc
     3481 gagcacgatt tgccgcgtca agcgaaggtg ccgctcgagc tccgagcggc cgatccggcg
     3541 ctgcgcgcgc tgctcgaacg cgaatcgcgc tacgtcgagc tgctcgtcgg gaccgacggc
     3601 gcgccgaagg tcgccgcgcc gagcccggag cggccgcgcg gctttttggt gtccactgcg
     3661 ggcgacgtcg aggtgctcgt cggtctgcgc ggcctcgtcg aggcgaagaa agaagaagag
     3721 cgcctgtcgc gtcagctgaa gaagaccgaa aaggacatca aggtcctcga aggtcgactc
     3781 gccaacaaga acttcgtcga caacgctccg cccgaagtcg tcgccgagtc gaaggcgctg
     3841 ctcgagcaga tgaagcggca gttccagcgc attcaagagg cgcaagcgct cgtccacgag
     3901 ctcgagtgag cgctcgcttc aattgaaggc gctgcgctga gccaggcgcg accgggggcg
     3961 gcctgtgcgc cccccacgaa ccggacgcct cagaaccgga agcgaccgag catgcccgct
     4021 tctcgaccgt cggggttccc gccgcggccg agcaagttgt aaaagcggaa gtacgcgccg
     4081 agatccacgc gctcgccgat gcggaagagg acgccgcctt cgaccggtac ggagaggtta
     4141 tcgccgaagc cgcgcacggg gcctaccagc atcgcgccga cgaacggcac gacttgccgc
     4201 gtcgcctgga tgtagaccca aaagggcagc acgagctcgt cgccgttgcc ccgatcccgg
     4261 cgattgaacc cgacgtacac ggacgggtcg aacacgaacg cgactcgatc gccgctgatc
     4321 cggccgatga ccccgccttt ccacccaacg tagaaggggt cgagacttcg cgactggatc
     4381 ccggtgttga tgtcgagctc gaagccgtgc ttttgaacga gcccgaccag aagcccaaaa
     4441 cccacgtcgt tgtacgctcg ctcacagccc gtcacgcaca ggccgtagcg gtgcgtgatg
     4501 ccgatcgtga cgtgatccga tacgccccag taaaagtgcg gtgcgagcca gatcggatgc
     4561 ggcgagtggt tggatacgtc gattcgtacg atttgaggac gcgagggttc gccggtgatc
     4621 tcgaagtgtc cgtcggtcgt gagaaagctg cggtgcgtgt agtcttgcgc cgacgcgcga
     4681 atggtcgaaa gcgagacagc gatgaacgcg agcggtccga gagtccttgc taattgcatg
     4741 cgcgaggcga gcctagacgt gccacgcgcg gcgcgtaaag tgtgcggata gcgcgccccg
     4801 aatgacacgc gcgaatcgcc cgctgaaacc ggcgaaggag cctttccgcc gcacgtgtgc
     4861 cgagagctcc gacagcgaaa accgcagcca atacttgcgt gttggcttgc ggagacgctg
     4921 aatccgggta gtttaattgt tgaggccgtt cccggtgccg aaccgagatc ccaagctatc
     4981 cccgcgtcta cccaaagaca tgcccgttga acgtgaagag tcgacgggcg ttggcgtttc
     5041 gcggagcgag gcggcggcgc tggccgtgct ggacgagcct accgacctcg tgcctgcgtt
     5101 cgagcatgac gacgacgagt cgaccgcgct cgtctcgctc gagagcctgg ggcgcatcga
     5161 gcatcacgag ccgaaggatc gccatttgct cgtgcgggtg aagggcgcag gcctcggtca
     5221 agtcacgcgc ctcggcgaca agccttgcta cgtcggtcga ggcagcgaag ccggccttta
     5281 cgtgagcgac agcggcgtca gccgcaagca cgcgatgctc atccccgacg gggagactta
     5341 cgtgctcatc gacaccgaga gtgcaaacgg cactttcgtg ggtggtcagc gcgtttcgcg
     5401 gcacgtgctg cgggacggtg acgtggtgca gttcgcttcc accgcgtcgt ttcgctacac
     5461 gctgaccgac gcgagccaag aggcgctgct gcgtcagttg ttcgaagcga gcgtcaccga
     5521 cgcgctgacg ggcgccttca accgcgatca cttcgacacg cagctcgcgg cggagctcag
     5581 ctacgcgcgg cgccacaaga ccgagctctc gctcgtgatg ttcgacgtcg acttcttcaa
     5641 aaaggtgaac gacacgtacg gtcacccgtc gggcgaccaa gtgctcgtac gcatcgcccg
     5701 caccgtcgcg gggatggtgc gcaccgaaga cgtctttgcg cgctacggcg gcgaggagtt
     5761 tgccctgatt ttgcgcggcg tgaacgtagc cggggccgcg gctctcgccg agcggctgcg
     5821 aggcgcagtg caggcattga cgatcccgag cgatcgcggg ccgatccgcg tgaccatcag
     5881 cgcggggtgc gccgcggtga gcggcggtgc cgagatcacc gccgaagagt tggtgggcgc
     5941 ggccgacaag cggctctacg ccgccaagca cggcggccga aatcgcgtgc actcggccga
     6001 ctgagccggc gccagagctt gcccgcgaac gacgcctgtt ttacgaacgc ggctcgatgc
     6061 cgcccgggca agagctagat agcgaacccc gcgcgcgctt gccgagcctg ctcggtgcca
     6121 tcgtggccgg gctgttgctc atcgcgctga tgagcaggga agggcatccg gggctctcgg
     6181 tccccctggg gctcgggctg gtgctcgcgg cgagcttcgg gctcttgtac gggctcggct
     6241 tcttcacgaa aagcgaggtg gtggggcgca gcgtgcccgt gcgcgagctc gccgcgccct
     6301 ttgcgacgac gctcgcatcg gtcgtcgccc tcgtcgtcgc gttgcggctc gcggtggccg
     6361 gcggcttgcc gatgccgctg ctgagctcgg ccgtcttggt cacctcgacg agccttgcgt
     6421 gtgtgttttc gctcggctgg tgctgcgacg tcgctcgcat cgacccggaa gcgcctccgt
     6481 tctggcgtcg tcacggcgtt tggctgctcg cgattgcgat cgtcgcgtat gtgccgatgc
     6541 tcggcaacta ctcgctctat gatccgtggg agacgcacta cggtgaagtc gcgcgcgaga
     6601 tgctggcgcg cgaggactgg attagctttt ggtgggcgca agagaattgg ttttggtcga
     6661 aaccggcgct cgacttttgg cttcaggggc tgtcgtttct attgctcggc gtgcgctatc
     6721 gcccggacga gatgctggcc ggcgtcgcgg acgggctcaa cccagcgccc gagtgggctg
     6781 cgcgcgcgcc agtgatcttg ctgacgctcg tcgcgaacta tctgctctac aaggcggtcg
     6841 cgcgctttct ggggcggcgc gtcgggttcg tcgctgggct cgcgctcacg ctggcgccgt
     6901 actggtacct catcgcgcat cagagcatga ccgacatgcc gtacgcagcg ccgctcaccg
     6961 ccgccttggc gctactcttg attggcctcg acctgtcacc gagggcgacc gtcgcgtcga
     7021 tcggcgtgcg cgtcggtcga cgtgagcttt ggttcggcaa gcaccacgcc gtgctactgc
     7081 tcgtcgtcgc gtcggcgatt ccgcagatcg cgtatctgct gtcgcggcac gtgagctggt
     7141 cactcgccgg cgcgtcgcac ttcttgcgcg tgcacgccga ccagttcatg gcgggctcgg
     7201 gtggcgacaa ttgtgggcta ccgggcaacg atccctgcga ggcctcggcg gcggttttgc
     7261 cggagctccc gccggggctc atggccttgc tgtggatcgc tgcgctcgcc gccttcctgt
     7321 ggctcaatcg agaagagagc cgcgcccagc gctgggcgtt tttggccggt tggtatttcg
     7381 tagcggtctc cgccctcggc cagggcgcgc ctggtttggt tctgccagtc gtcgtggtcg
     7441 tcaccgcgct cttcgcgctg cgaaggctgc gcgatctgga gcggctcgaa tggggcgggt
     7501 tcttgctcat cgtcgcgtgc gtcaccctgc cttggtacgt gcaggcattc gcgcgacacg
     7561 gcacgggctt tacggaccgc ctcctgtttt acgacatgta caagcgcgcc ttcgttcacg
     7621 tgcatgacac gaacgcgggc gacgacgtga gctttcgtta ctacatctgg cagctcgggt
     7681 acggcttgtt cccgttcacg ggcgtcgcgc tctggggctt tctaaagagc ttcgcggtgc
     7741 gcccaaaggc cgaagcgaag acgcgcgacg tgctcgtgtt cttggggatt tggagcctgc
     7801 tcggcttcgc gatgttcagc gtctcgctca cgaagttcca ccactacgcg cttccggtcg
     7861 ctccgcccgc ggccgtcctc gccggcgtcg cgctgcacga attcgtgggg gacgcgcgca
     7921 ccgagaacgt gcgccaagcg gtcgttcgtt acggcgcggc ggcggcagcg gcggcgctcg
     7981 cccttgccgc aacggtgtgg ctcacgcagg gcagttggtt cggtgtgcgc acggagccca
     8041 acgtcctcag agcggcgctc ggcagccccg cgttcattgc cgcggtggca tgctttgccg
     8101 tttcggtgcg cttcggcggc ccgctcgcgg ctagcgcgcc ggtgaatc
//
LOCUS       DASTKS010000402         9006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_64160,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000402 DASTKS010000000
VERSION     DASTKS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64160"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFQ35_08325"
     CDS             <1..546
                     /locus_tag="VFQ35_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930679.1"
                     /translation="GPASACNLQRFTRVPLPPHAFAELLRGEAPVLVHDRASASIDWS
                     KGSYLVRIQSKNQAEQEIRLLPTDADYLKPFQEQRVRVLSVAVRQAGAPLYSVELDDH
                     APAAMATPRQDPDGIDAPIPPSGPECHAEVPRRIRFVVGDGERDLLLVSKDVTHNPPL
                     VAGVFTQEAPPGIRSSYSACE"
     gene            complement(570..1313)
                     /locus_tag="VFQ35_08330"
     CDS             complement(570..1313)
                     /locus_tag="VFQ35_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9930680.1"
                     /translation="MQKSSRFVRTAARPLVLGHRGARHAAPENTLRAFELARTEGADG
                     VELDVRVDRDGNVIVLHDPDLARVTAGARLGHVEELPPDEVRRADVGQGERVPLLRDV
                     LEWAEATEQCVNVEVKSDVRNRKALLSGVVRELEAMKRNELILLSSFDPRLVTALARR
                     LPQFGVCWLFHEKQTILRRLPGWRALGAIGVNPEHTLLSSYAVRRFKAQGALVNTWTV
                     NDPVLARAYGVFGVDSIISDCPGKILAEL"
     gene            complement(1319..2278)
                     /locus_tag="VFQ35_08335"
     CDS             complement(1319..2278)
                     /locus_tag="VFQ35_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930681.1"
                     /translation="MRFVVATHGHCFDGLASAALFTRLQRELGAGAAEFEYRACGYSP
                     GQPHADDAVLNGDDNAILDYRFSPSPRLTWYFDHHRTAFETPQDRAIFEGRNSPDRLF
                     HDAAYSSCTKLVADVARERFGVTMADLGDLVSWADRVDSARFESAEQAVSRSDPVMRF
                     VSVVEHYGDDAFFTRVVPQLLSQPLEELARSGEVERRFRPLGKKHDRFVQRVRDKGER
                     RGRVVFVDLTETVLDTVGKFVTYALFPDSVYSVIVGLLKSGPKISVGYNPWSGRPRDA
                     DISAICARYGGGGHPVVGGIAFRANEVERARLVANQIANELAG"
     gene            2404..3396
                     /locus_tag="VFQ35_08340"
     CDS             2404..3396
                     /locus_tag="VFQ35_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9930682.1"
                     /translation="MVLLGSTLPPRLKDLPNPPKCLFVRGELPRGPSVAIVGTRHPTA
                     EGSEFAHSLAGELARSGVAILSGGAEGIDTVAHEGALSVGGETAVIAPAGFSRPFPED
                     NAALFARVVAEGGAYASLVEDEVPARQGAFFARNACLAALGWALVVVEAGFRSGARNA
                     AAAARRLGRPVFAVPSAPWLPKGAGCIEELRLGARICAGAKDVLRSLEELGAQPIPPS
                     SKPRGRRRSPAPGELQLPLSVGSFSACCPTAALAEQTATPDVTSPPSSGDGEAELCER
                     VTLAIAAGAHEPGEVAELAGLSVAVVNRILLTLRLQGVLVSAASGYIYLLKSSD"
     gene            3499..5997
                     /gene="topA"
                     /locus_tag="VFQ35_08345"
     CDS             3499..5997
                     /gene="topA"
                     /locus_tag="VFQ35_08345"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9930683.1"
                     /translation="MSKTLVVVESPAKAKTIKKYLGAGYEVLASKGHVKDLPKRMGID
                     VEKGFKEQYEVIEAKAKVLTDLKHEAEKADVVLLATDPDREGEAIAWHLAEELERPKL
                     KMRRVEFEEITKKGVEKGLENPRDLDKHLYDAQRARRVLDRIVGYDVSALVWQKLAFG
                     LSAGRVQSVALRLIVEREREIEAFVPEEYWNVAVGLRGKAPQTVLARLSRADGEKVEV
                     KNGEHAAELREDLTTATYKVAAITQREQKRHAPAPYTTSKLQQDGTSYLRFTTKRTMS
                     IAQKLYEGVDLKKDGGLVGLITYMRTDSVRVSPDAINEVRELIKTQYGDKYLPAKPNV
                     YKSRKEAQEAHEAIRPTSLEYTPDRVAKHLTDEQFKLYKLIWDRFVASQMSNAVYDQT
                     GVDIEATPTRKAPRYKSITLRASGKVLKFSGWLEQYGKGLSSADGPKELAGEEDQAEG
                     KSEAEKARAAEEEEGLLPELAQGEVLKLHSPPGVVTEQKFTQPPARFNEGSLVRELEK
                     RGIGRPSTYAEIISKVQARDYVEKLPGGQMKATELGKMVIDGLVGTHLDFMDPSFTAK
                     MEEDLDEVEGGRLDRVALLSRFYQRFREVLDKAKKTARWTPEPEPTELKCDECGSQLN
                     KRWSKNGWFLGCSAYPKCKFTKDLGKDGNTPAEVRTTDIICDKCQKPMIIKTGRYGEF
                     LSCSDYPKCKNTKPVPLGVPCPKCGGDIVEIRSKKRGARSFYGCGNYPQCDFKLWQKP
                     VNEPCPECGSPFLVVGGGAKNPKLVCPKGKECGYSRPIEEGEMAAVGGAVPAEGADGN
                     GAPNGDGDGGDGKSEENPAGGGKRSRSRSASATP"
     gene            6004..7347
                     /gene="trmFO"
                     /locus_tag="VFQ35_08350"
     CDS             6004..7347
                     /gene="trmFO"
                     /locus_tag="VFQ35_08350"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET9930684.1"
                     /translation="MASVSIIGAGLAGSEAALTLANHGIDVVVYEQKPEARTPAQTSD
                     SLCELVCSNSFRGAALSNAVGLLKEEMRRMGSFVMSAAEETRVPAGGALAVDRDKFSA
                     RMTELLRSNPRIQIRSGRVDSIPPERPLVIATGPLTGDALANDIARVLGADALAYYDA
                     IAPIVSAESIDWSKVFMASRWDKGEDEEDKSAYVNCPLDEQAYFDFVREVRAARKVEP
                     KAFEQVRYFEGCLPIEVMAERGEQTLAFGPMKPVGLTDPRTGRRPYAVVQLRKEDQAG
                     SAYNMVGFQTRMAWPEQQRVFRMIPGLENAEFQRFGAVHRNTFVNAPKFLEPTLECRL
                     LPGVYFAGQIAGTEGYVESAGLGLLVALILVDRLSGREPLLPPVETALGGLLTHLARH
                     PEDYQPSNITFSHLPPWEGSRLKKRMKYEAMAERSLSALAEWSRRRNGQAELVAG"
     gene            7436..>9006
                     /locus_tag="VFQ35_08355"
     CDS             7436..>9006
                     /locus_tag="VFQ35_08355"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9930685.1"
                     /translation="MLSSDALVDIVQAYHPSADGDLIRRAYQFAEWAHKEQLRKSGDP
                     YLIHPVSVAGIIADLRLDTASVCAGLLHDVVEDSAATRETIEGEFGSEIASIVDGVTK
                     LGKFNFNSREDRQAESFRKMVVAMAQDLRVLLVKLCDRLDNMRTLDHMKPDAQERIAR
                     ETMDIYAPLAARLGMQRIKSELEDLSFKHLEPDAYKELAQKLQRTKKDRDRYIESVCR
                     TITSRLAEQGYAAEVTGRAKHLYSVYRKMKEQQCEFEQVFDILAFRIGVESVADCYAA
                     LGAMHSRWTPIPGRFKDYVALPKPNMYQSLHTTVIGPARQRIEIQIRTHEMHRVAEHG
                     IAAHWKYKERGSGGVDPKDAEKFSWLRELAEFQRTLKDPVEFLESVKIDLFPDEVYVF
                     TPKGELRVLPRGSTPIDFAYAIHSQVGEHCSGARANGQIVPLRYKLKNGDVMEIMTSP
                     QQEPSKDWLEFCVTTRARSRIRAKLRAEQRTKSVNLGKELLESEMRDAGLSFAKFTRN
                     DAEIRRVAEQFGHNHL"
BASE COUNT         1724 a         2938 c         2895 g         1449 t
ORIGIN      
        1 gggcccgcga gcgcctgcaa tctgcagcgg ttcacgcgcg taccgctgcc cccgcacgcc
       61 ttcgctgagc ttctgcgggg cgaggcgccg gtcctcgtgc acgaccgggc gagcgcgagc
      121 atcgactggt cgaaaggcag ctacctcgtt cgcatccaaa gcaagaacca ggcagagcaa
      181 gagattcgtc tcttgccgac ggacgccgac tacttgaagc ctttccaaga gcagcgcgtt
      241 cgagtgctca gcgtcgccgt acgccaagcg ggcgcaccgc tctacagcgt cgagctcgac
      301 gaccacgctc cagcggcgat ggcgacgcct cgccaagatc ccgacggcat cgacgcgcca
      361 attccgccga gcggtcccga gtgccacgcc gaagtgccgc gccgaattcg cttcgtcgtc
      421 ggcgatggtg agcgcgatct gctcctggtc agcaaggacg tcacccacaa tccgccgctc
      481 gtggccggtg tgttcacgca agaggctccg cccggaattc ggtcgagtta ttccgcttgc
      541 gagtgagctc ggctaccaat gcccgatttt cagagctcgg ccaggatctt tccggggcag
      601 tcgctgatga tcgagtcgac accgaacacg ccgtaagcgc gggcgagtac cggatcattc
      661 acggtccacg tgttcacgag agcgccttgg gccttgaacc gacgcaccgc atagctcgag
      721 agcagcgtgt gctccgggtt cacgccgatg gcgccgagcg cgcgccagcc cggtaagcgc
      781 cgcaagatcg tctgcttctc gtgaaagagc cagcagaccc cgaactgagg caggcgccgc
      841 gcgagcgcgg tcacgagccg ggggtcgaag ctcgagagca ggatgagctc gttgcgtttc
      901 atcgcttcga gctcccgcac gacgcccgag agcagcgctt tgcggtttcg aacgtcggac
      961 ttcacctcga cgttcacgca ttgctccgtg gcttccgccc actcgaggac gtctcgcaac
     1021 agcggtacgc gctcgccctg acccacatcc gcgcggcgca cttcgtccgg cggaagttcc
     1081 tcgacgtggc cgagccgggc ccccgcggtc acacgcgcca gatcggggtc gtgcagcacg
     1141 atgacgttgc cgtcgcgatc gacccgcacg tcgagctcga cgccgtccgc gccctccgtg
     1201 cgcgcgagct cgaaggcgcg cagcgtattt tcgggagcgg catggcgtgc gccccggtga
     1261 ccgagcacca gcggccgcgc ggcggtccgc acgaagcgag acgacttttg catcgagttc
     1321 agccggcgag ctcgttcgcg atctgattgg cgacgaggcg agcccgctcg acctcgttcg
     1381 cgcggaacgc gatgccgccg acgacggggt gcccaccacc gccgtagcga gcgcagatgg
     1441 cgctgatgtc cgcgtcgcgt ggccgccccg accacggatt gtatccgacc gaaattttcg
     1501 gaccgctctt caggaggccg acgatgaccg aataaaccga gtccgggaac agcgcgtacg
     1561 tcacgaactt gccgactgta tcgagcaccg tctcggtgag gtcgacgaac acgacgcgcc
     1621 cgcggcgctc gcccttatcg cgcacccgct gcacgaagcg gtcgtgcttc ttgccgagag
     1681 ggcggaaccg cctctcgact tcaccagagc gcgcgagctc ttcgagcggc tggctcagga
     1741 gttgcgggac gacgcgcgtg aagaaggcgt cgtcgccgta gtgctcgacc acgctcacga
     1801 aacgcatcac cggatccgaa cggctgaccg cctgctcggc gctctcgaac cgcgccgaat
     1861 cgacccgatc ggcccagctc acgagatcgc ccagatcggc catcgtcacg ccgaaacgct
     1921 ctcgcgcgac gtcggcaacg agcttcgtgc acgagctgta ggcggcatcg tgaaagagcc
     1981 ggtcggggct gttgcgccct tcgaagatgg ctcggtcctg cggcgtctcg aacgccgtcc
     2041 gatggtgatc gaagtaccag gtgagccgcg gcgaaggcga gaagcgatag tccaagatcg
     2101 cgttgtcgtc gccgttcagt acagcatcat cggcgtgcgg ctgccctggg ctatacccgc
     2161 aagctcgata ctcgaattca gcagcccctg caccaagctc gcgctgaagt cgagtgaaga
     2221 gagctgcact cgccaggcca tcgaagcagt ggccgtgggt tgctaccacg aaccgcatgt
     2281 cgcactgtct accacacagg agtcgcacga aggaaagccc tatcgtcctc gtccggtcgc
     2341 tttcatttca cgcaactccc agccgaaatc cgccgatcgt ggggggtgaa atcaattctg
     2401 cccgtggttc tgctgggttc cacgcttccg ccacggctca aggacctgcc gaaccccccg
     2461 aaatgcctct tcgtccgcgg agaattaccg cgggggccga gcgttgccat cgtagggacg
     2521 cgccatccca ccgccgaggg gagcgagttc gcccattctc tagctgggga gctcgcccgc
     2581 tcgggcgtgg cgatcctttc gggcggggcg gaaggcatcg acacggttgc tcacgaaggc
     2641 gctctttcgg tgggcggaga gaccgcggtg atcgctccgg ctggcttctc gcgacccttt
     2701 ccggaagaca acgcagcgct cttcgcgcgc gtcgtggccg aagggggcgc ctacgcatcc
     2761 ctcgttgagg acgaggtacc cgcgcggcaa ggcgcttttt tcgcccgcaa tgcctgcctg
     2821 gcagcgcttg gctgggccct cgtcgtcgtc gaggctgggt ttaggagcgg cgcacgcaac
     2881 gccgccgcgg cagcgcgacg gctcgggcgc ccggtgttcg cggtgccctc cgcgccctgg
     2941 ctgcccaaag gtgcgggatg catcgaggag ctgcggctgg gtgctcgtat ttgtgcaggc
     3001 gcgaaagatg tcttgcggtc cttggaagag ctcggtgcgc aaccgattcc gccctcatcg
     3061 aagccccggg ggcggcgccg cagccctgcg cccggcgagc tgcagcttcc gttgagtgtc
     3121 ggttctttct cggcgtgctg tccgacggcg gcgttggcgg aacagaccgc gacaccggac
     3181 gttacttctc ctcctagtag tggggatgga gaggccgagc tctgcgaacg agtcaccctt
     3241 gcaattgccg ccggagcgca cgaaccaggc gaggtggcgg aattggccgg tctgtcggtt
     3301 gccgtcgtca accgcattct cttgactctg aggctgcaag gcgtcttagt ttcggccgcg
     3361 tccggataca tctaccttct taaatcatcg gattaacagg ggttaggttg ctgggccgat
     3421 tttcccgcgg ggggatcccc cttgcgggac ccatgtttcg ggctcattga ggaactcccg
     3481 ctggaagaaa accgggccat gtccaaaacc ctcgtagtcg tcgagtcccc cgccaaggcc
     3541 aagaccatca agaagtatct tggtgccggc tatgaggtgt tggccagcaa gggccacgtc
     3601 aaagacctgc ccaagcgcat gggcatcgac gtcgaaaaag gcttcaagga gcagtacgaa
     3661 gtcatcgaag ccaaagccaa ggtgctgacc gatctgaagc acgaggcgga gaaggcagac
     3721 gtcgtcctgc tcgcaaccga ccccgatcgc gaaggcgaag cgatcgcttg gcacctcgcc
     3781 gaggagctcg agcgcccgaa gctgaaaatg cggcgcgtcg agttcgagga gatcaccaaa
     3841 aagggcgtcg aaaaggggct cgaaaacccc agggacctcg acaagcacct ttatgacgca
     3901 cagcgcgctc gtcgcgtcct cgaccgtatc gtcggctacg acgtgtcggc gctcgtttgg
     3961 cagaagctgg ccttcgggct cagcgccggc cgcgtgcagt cggtggcctt gcgcctcatc
     4021 gtggagcgcg agcgcgagat cgaagcgttc gttccggaag agtactggaa tgtcgccgtc
     4081 gggctgcgcg gcaaggcgcc gcaaacggtc ctcgctcgcc tgagccgcgc cgatggcgag
     4141 aaggtcgaag tcaaaaacgg cgagcacgcc gccgagctgc gcgaagatct gacgacggcc
     4201 acttacaagg tcgccgcgat cacgcagcgt gagcagaagc ggcacgctcc tgcgccgtac
     4261 acgacgagca agcttcagca agacggcacg agctacctgc gcttcaccac caagcgcacg
     4321 atgagcatcg cgcagaagct gtacgaaggc gtcgacctga agaaggacgg cggcctcgtc
     4381 ggtctcatca cctatatgcg tacggactcg gtgcgcgtga gccccgatgc catcaacgag
     4441 gtgcgcgagc tcatcaagac gcagtacggc gacaagtacc tgccggcgaa gcccaacgtc
     4501 tacaagtcgc gcaaagaggc tcaagaggcg cacgaagcga tccgcccgac atcgctcgag
     4561 tacacgccgg accgcgtggc aaagcacctc accgacgagc agttcaagct ctacaagctg
     4621 atttgggatc gcttcgtcgc ttcacaaatg tccaacgccg tctacgacca gaccggcgtc
     4681 gacatcgaag ccacgccgac gcgcaaggcg ccccgctaca agagcatcac gctgcgcgcg
     4741 agcggcaagg tgctcaagtt ctcgggctgg ctcgagcagt acggcaaggg cttgagcagc
     4801 gccgacgggc cgaaggagct cgcgggtgaa gaggaccaag ccgagggcaa gagcgaagcc
     4861 gaaaaggcgc gcgccgccga ggaagaagaa gggcttttgc cggagctcgc tcagggcgag
     4921 gtgctgaagc tccattcgcc gcccggcgtc gtcaccgagc aaaagttcac ccagcccccc
     4981 gcgcgcttca acgaaggttc gctcgtgcgc gagctcgaga agcgcggcat cgggcgtccc
     5041 agcacctacg ctgaaatcat cagcaaggtg caggcgcgcg actacgtcga gaagctgccc
     5101 ggcggtcaga tgaaggcgac cgagctcggc aagatggtga tcgacgggct cgtcggcact
     5161 cacctcgact tcatggatcc gagcttcacc gccaagatgg aagaagatct cgacgaggtc
     5221 gaaggcggac gactcgatcg cgtagcgctc ttgtcccgct tctatcagcg cttccgcgag
     5281 gtgctggata aagccaagaa gaccgcgcgt tggacgccgg agcccgagcc cacggagctc
     5341 aagtgcgacg agtgcggctc tcagctcaac aagcgctgga gtaagaacgg ctggttcttg
     5401 ggttgctcgg cgtacccgaa gtgcaagttc acgaaggacc tcggcaagga cggcaacacc
     5461 ccggccgagg tccgcaccac cgacatcatc tgcgacaagt gccagaagcc gatgatcatc
     5521 aagacggggc gctacggcga gttcctgtcg tgcagcgact acccgaagtg caagaacacg
     5581 aaacccgtgc cgctcggcgt gccctgcccg aagtgcggcg gtgacatcgt cgagattcgc
     5641 tcgaagaagc gcggcgctcg ttcgttctac ggctgcggca actaccccca gtgcgacttc
     5701 aagctctggc aaaagccggt gaatgagcca tgccccgagt gcggcagccc gttcttggtg
     5761 gtcggcggcg gcgcaaagaa tcccaagctc gtctgcccga agggcaaaga gtgcggctac
     5821 tcgcgtccca tcgaagaagg cgagatggcg gccgtcggcg gcgcggtccc cgcggaggga
     5881 gcggacggca acggcgcccc gaacggcgac ggggacggcg gcgacggaaa gtcggaagaa
     5941 aacccggccg gcggcggtaa acgcagccgc tctcgttcgg cgtctgctac gccctgagcc
     6001 gacatggctt ctgtcagcat catcggtgcg ggcctcgcgg ggtccgaggc cgcgctcacg
     6061 ctcgcgaacc acggtatcga cgtagtcgtc tacgaacaga agccggaggc acgcacaccg
     6121 gcgcagacta gcgattcgtt gtgtgagctc gtctgctcga attcgtttcg cggcgcggcc
     6181 ttgagcaacg ccgtcgggct cctcaaggag gagatgcgac gcatgggttc gttcgtgatg
     6241 agcgccgccg aagagacgcg cgtgcccgcc ggcggcgcac tcgcggtcga tcgagacaag
     6301 ttctcggcgc gaatgacgga gctcctgcgc tcgaacccgc gcattcagat tcgctctggt
     6361 cgggtcgatt caatcccgcc ggagcggccg ctcgttatcg cgacagggcc cctcactggc
     6421 gacgcgctgg cgaacgacat cgcacgggtg ctcggcgccg acgcgctcgc ttactacgac
     6481 gccatcgcgc ccatcgtcag cgccgaatcg atcgattggt cgaaggtctt catggcctcg
     6541 cgctgggaca agggcgaaga cgaagaagac aagagcgcct acgtcaattg ccctctcgac
     6601 gagcaggctt acttcgactt cgtgcgcgag gtgcgcgccg ctcgaaaggt ggagccgaag
     6661 gcgttcgaac aggtccggta cttcgaaggc tgcctgccaa tcgaggtcat ggcagagcgg
     6721 ggcgagcaaa cactcgcgtt cggccccatg aagccggtgg gcctcaccga tccgcgcacc
     6781 ggtcggcgtc cctacgccgt cgttcagctt cgcaaggagg accaggccgg cagcgcgtac
     6841 aacatggtgg gttttcaaac ccgcatggcg tggcccgagc agcagcgtgt ctttcggatg
     6901 atccccgggc tcgagaacgc cgagttccaa cgctttggcg cggtgcatcg caacaccttc
     6961 gtcaacgccc ccaagttcct ggagccgacc ctcgagtgtc ggctattgcc aggggtgtac
     7021 ttcgcgggcc agatcgcggg gaccgagggc tacgtcgaga gcgccgggct gggacttctg
     7081 gtcgcgctca tcctggtcga ccgcttgtcg ggacgtgagc cgctcctgcc gccggtcgaa
     7141 acggcgctag ggggattgct cacccacctg gcccgacatc cggaggacta ccagccctcc
     7201 aacatcacct tttcccatct gcccccttgg gaaggcagcc gcctaaagaa gcgcatgaaa
     7261 tacgaggcca tggcggagcg aagcctctcc gctttggccg agtggagccg ccgccggaac
     7321 ggccaggcag agctcgtcgc cggataaccg gcggaaaccg cgatactttt caggcagatg
     7381 gcgcgcccgt agcggagccc atactcgggt gttactttag ggtgacgagc cctccgtgct
     7441 gtcatccgac gccctcgtag acattgttca ggcctatcac ccctcggccg atggcgacct
     7501 aatccggcgc gcgtaccaat ttgccgaatg ggcgcacaaa gagcagctgc gcaaaagcgg
     7561 cgacccttac ctgattcacc ccgtcagcgt agccggcatc atcgcggact tgcggctcga
     7621 taccgcgagc gtgtgcgcag gactactaca cgacgtcgtc gaagatagcg ccgcgacgcg
     7681 cgaaacgatt gaaggggagt tcggatccga gatcgccagc atcgtcgacg gcgttaccaa
     7741 gctcggtaag ttcaatttca attctcgcga agaccgccaa gcggagagct tccgcaagat
     7801 ggtggtcgcg atggcgcaag acctgcgcgt gctgctggtc aagctctgcg accgcctcga
     7861 caacatgcgg acgctcgacc acatgaagcc cgacgcgcaa gagcgcatcg cgcgcgagac
     7921 gatggatatc tacgcgccgc tcgctgcacg cctcggcatg cagcgcatca agagcgagct
     7981 cgaagacctg agcttcaagc acctcgagcc cgacgcttac aaggagctcg cgcagaagct
     8041 ccagcgcacc aagaaggacc gagatcggta catcgagagc gtctgccgta cgattacgtc
     8101 gcggctcgcc gagcaaggct acgccgccga agtgaccggc cgcgccaagc atctctactc
     8161 cgtctatcga aagatgaagg agcagcagtg cgagttcgag caggtgttcg acatcctcgc
     8221 gttccgcatt ggggtcgagt cggtcgcaga ctgctacgcc gcgctcggcg cgatgcactc
     8281 gcgctggacg ccgatccccg ggcgcttcaa ggactacgtc gcgctgccga agccgaacat
     8341 gtaccagtcg ttgcacacga cggtgattgg cccggcgcgt cagcgcatcg aaatccagat
     8401 ccgcactcac gagatgcacc gcgttgccga gcacggcatc gccgcccatt ggaagtacaa
     8461 ggagcgcggc tcgggcggcg tcgatcccaa agacgcggag aagttcagct ggctgcgcga
     8521 gctcgccgag ttccaacgca ctctcaagga tccggtcgag ttcctcgaga gcgtcaaaat
     8581 cgatctgttc cccgacgagg tgtacgtctt taccccgaag ggcgagctcc gagttttgcc
     8641 gcgcggctcg acgcccatcg acttcgcgta cgcgattcac tcgcaggtgg gcgagcactg
     8701 ttcgggcgcg cgcgcgaacg gccagatcgt tccgctccgc tacaagctga agaacggcga
     8761 cgtgatggag atcatgacgt cgccgcagca agagccgagc aaggactggc tcgagttctg
     8821 cgtcaccacg cgcgctcggt cgcgcatccg cgccaagctc cgtgccgaac agcgcaccaa
     8881 gagcgtgaac ctcggcaagg agctgctcga gtcggagatg cgcgacgctg gcctcagctt
     8941 cgcgaagttc acccgtaacg acgccgagat ccgccgcgtc gccgagcagt tcgggcacaa
     9001 ccacct
//
LOCUS       DASTKS010000403         8331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_732296, whole genome shotgun sequence.
ACCESSION   DASTKS010000403 DASTKS010000000
VERSION     DASTKS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732296"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFQ35_08360"
     CDS             complement(<1..224)
                     /locus_tag="VFQ35_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930686.1"
                     /translation="MLRGAVVRFAGDSGDGMQVTGDQFTVAAALARNDLSTFPDFPAE
                     IRAPAGTTYGVSGFQIHFASEHVYTPGDAP"
     gene            complement(477..923)
                     /locus_tag="VFQ35_08365"
     CDS             complement(477..923)
                     /locus_tag="VFQ35_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930687.1"
                     /translation="MTSLSEQLGSGAKRDAVINDALKVLDEEVADKSGLTGLAIKGGY
                     KVVQGIKPGFVRQVVESLLPEFLKALDPVYAEAVEKKRPAGAYLLEQKSRVADALLAV
                     TDQKAQRASGAIRSAYDKLRPMAKKQVEAAAPRLATMLERHAAPTA"
     gene            1309..2556
                     /locus_tag="VFQ35_08370"
     CDS             1309..2556
                     /locus_tag="VFQ35_08370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930688.1"
                     /translation="MTEELRQGSRLGENELVLRIGRGGMATVWVARERNADPSLERLV
                     AVKAMLAELADEPEFTRMFIDEVRLVRSIRHPNVVEIFDVGESEGVMWMSMEWVQGES
                     LHTIIAEAGKRRAIPPEMAVRIIADVAAGLNAAHELRDASGALRGVVHRDVSPHNILI
                     GTNGAIKLVDFGVAKALGRVSESTRAGQLKGKFGYMSPEQALGRPVDRRSDVFSLGIV
                     LFELTTSRRLFRGESDVETLKLVISGQVPSPSSIDPSYPPELERIVMRALERDPRLRY
                     QTAAEFEQDLRGFLKAERVVVPQSGVAGLLKRVVGERIEQRRRAIRAALKKIGSGGAG
                     GGDLLTTETAFTPTGKDRISVTGTDPSSAGMGEFPDRHLRRAQDRRSPRPQDGVNWSS
                     AIGYAIGVAGLIIAIIVLLFLSR"
     gene            2807..4156
                     /locus_tag="VFQ35_08375"
     CDS             2807..4156
                     /locus_tag="VFQ35_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9930689.1"
                     /translation="MGKILVVDDQRNMRTTLAMMLRGAGYEVDEAADGAQGRESGATG
                     AYDVVITDLRMGEYDGIDVLRAVKEAQPMTEVIVMTAYGTIESAVEAMRVGAFDYIQK
                     PFTEQELLVKVSKALESRRLNGQVQLFAQDFKERFHVDNVIGRSQAIRDVLMRLIKFA
                     PTDATVLITGESGTGKELIARAVHANSKRAQRPFVTVNCAAISETLLESELFGHARGA
                     FTGAIGARKGLFEEADGGTFFFDEIAETTPVFQAKLLRAIQEGEIRRVGENKPIRVDI
                     RVVAATNVDLSRAVEERRFRQDLYYRLNVARFQLPPLRERREDIPLLAEHFIAKYSRK
                     MTKRVRFADGVMERLVAYDYPGNVRELENLVEQSVALCTGSVVRLDDILPRPPKSEEK
                     AHGRTLADIVDDAEREAVESALRAFDGSRERAAEALDISPTTLWRKMTRLGISFEDR"
     gene            4216..5256
                     /gene="queA"
                     /locus_tag="VFQ35_08380"
     CDS             4216..5256
                     /gene="queA"
                     /locus_tag="VFQ35_08380"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET9930690.1"
                     /translation="MKTELLDYPLPDALIARHPPHERDGGRLLVLDGARRTHHSVRDF
                     AELVPEGALVVLNASRVRRARVFGQRRPGGGRVELLFLEQSGRSEDGRERWAAIGRAS
                     KPLRPGVVIEAEGLEVEVAANDAGSLTLLARANEPLETLLDRIGHVPLPPYLGRGDEP
                     EDVERYQTVYAERTGSVAAPTAGLHLTEAMLARMRARGVDVHRLELAVGIGTFRPVSA
                     EDLDAHPMHEERFSVSAALVRAVAETKARGLPVVAVGTTVVRALESAAASGELVACEE
                     STRLLIQPGYRFRVVDSLLTNFHQPKSTLLALVAAFAGYDAVMAAYAEAIEQKYRFLS
                     YGDAMFIPRRAS"
     gene            5253..6398
                     /gene="tgt"
                     /locus_tag="VFQ35_08385"
     CDS             5253..6398
                     /gene="tgt"
                     /locus_tag="VFQ35_08385"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9930691.1"
                     /translation="MITFRVEARSGVARAGVLTTPHGEVRTPAFQPVGTQATVKALSA
                     EEVAATGARLVIMNTYHLWQRPGPELVAERGGLHAFSRWPHSITTDSGGFQAFSLAER
                     TRVDEDGFEFSSHLDGRRLRLSPEEAMRIQGLLGSDIAMQLDVCAPAGAARRELELAV
                     ERTTRWAERCLAAKFPEQALFGIVQGGLDVELRLAHAEALAKLPLDGLALGGFSVGEP
                     IEAMHETLARVAPTLDATRPRYLMGVGTPLDIVRAISAGVDMFDCVMPTRNARNGQAF
                     VPGGKLVIKNARYRNDPRPLDEDCTCDACRGGYSRSYLRHLYVAGEILALRLLTHHNI
                     QYYQSLVAGARAAILADRFGAWAETTLSALSHIQRDAAESTDTPAGR"
     gene            6721..8202
                     /locus_tag="VFQ35_08390"
     CDS             6721..8202
                     /locus_tag="VFQ35_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930692.1"
                     /translation="MSEKKQIGKILLRQRALRAEDLDRALGTEGHGRLASRLAAEGTI
                     SDVAALKALSEQHGVPGIDLSQICIRLEHLDLLPREIAEKHRILPVLERDERLFVAMA
                     DPRERSVIDELEFVTGKRVYPYVALDAALANVTAQAYTRKSRGESFYVGSHCPPELLQ
                     KLGIDANGEPIAESVVPLELIAPSGAPGSLGAEAVVVDDDVDRVSRDDDIEERDFGDA
                     SREHSVVGPSDAAELNPDRASKVILVVDDEAEIRRMLTHLLSRAGYKVIEADKGELAL
                     RMVREQAPDLIVLDAMLPEVHGFEIARRIKKSERYGHIPIIMVSAVYRGWRYAEDVKR
                     SFGVDHFVEKPFRISEIVQSVENMLKVRAESAPPPSDRDLSAAAEAALNAGIEAYQAG
                     NIELAIEHLKRGTTIDPLAFRLHFHLGLLYGKQGQVFDAISELEAAVESNGQHFAALK
                     NLAILYQKAGFRNRAAEMWERALPLAPDDSTRQSIRQHLLKLL"
BASE COUNT         1412 a         2711 c         2809 g         1399 t
ORIGIN      
        1 tcgggcgcgt cgcccggcgt gtagacgtgc tccgatgcga agtggatttg gaacccggag
       61 acgccgtacg tcgtgccggc gggtgcccga atttctgcag ggaagtccgg aaacgtcgaa
      121 aggtcgtttc gcgctagcgc cgcggcaacc gtgaactgat cgccggtgac ctgcatgccg
      181 tctccggagt cgccagcaaa gcgcacgacg gcgccccgga gcacggtgtg agaactaggt
      241 tgggggacgg gagaacttga ggtcatggcg cagaaaccgc acgaaaattg ggggcgcggc
      301 ccaggttgtc aagagaaccg atggaaaaac tggcgtttcg aaacgctctg tagcgaccgt
      361 tcgaaacccg gcggatacgt ttcctaacgc cggctccggg cgaatgctct agaaatctcg
      421 cgtgggcgcg cacgagcgcg ctagagacag cactcggcct tctggccggc gctgtgtcac
      481 gccgtcggcg cggcgtgccg ctcgagcatg gttgcgaggc gcggggccgc cgcttcgacc
      541 tgcttcttag ccatggggcg aagcttgtcg tacgcgctcc gaatcgctcc gctagcgcgc
      601 tgcgcctttt ggtccgtaac cgcgagcagg gcgtcggcga cgcggctctt ttgttcgagg
      661 aggtaggcgc cagcgggacg cttcttctcg accgcttcgg cgtacacagg gtcgagtgcc
      721 ttgaggaact cggggagcag cgactcgact acctgacgca cgaagcccgg cttgatgccc
      781 tggaccacct tgtagccgcc cttgatcgcg agcccggtga gaccgctctt gtccgccacc
      841 tcttcgtcca gcaccttgag cgcgtcgttt atgaccgcgt cgcgctttgc gccgcttcca
      901 agctgttcac tgagactcgt catagttctg tccgcctctt gctgctcgcg tggatttcct
      961 cgagcccgcc gccagcaacg cagcgagcgc gtcggcgggc tattagcact actccgcgaa
     1021 ggtgggcatg aaagcgcgtc tctcccaccg tggtgagttc ttcttcggac tgtcgttcgg
     1081 tcgcagccat gtgtccgagt tgccggctgc ctcttccgct tcgcctaatc tctggctagt
     1141 tggaaagacg gcggtcacaa tcgggctcac aaagtcggcc gccagcccga gtccccgcca
     1201 gaatagacag tcgcttcccc caaagccctc tcgttccgag gttcgccgag ttcgccgact
     1261 tcggaccgcg tttcgagggc gcagtcgaat ctcgcccccc gcaaatcgat gaccgaagag
     1321 ctcaggcaag gcagccgact tggcgagaac gagttggtgt tgcgcattgg tcgcggcggc
     1381 atggcgacgg tttgggtcgc tcgtgagcgc aacgccgatc cttcgctcga acggctggtc
     1441 gcggtgaagg ccatgctcgc cgagctcgca gacgagcccg agttcactcg aatgttcatc
     1501 gacgaggttc gtctcgtccg gagcatccgc cacccgaacg tcgtggagat cttcgacgtg
     1561 ggcgagagcg aaggggtgat gtggatgtcg atggagtggg tgcagggtga gagcctgcac
     1621 accatcatcg ccgaggccgg caagcgccgc gcgatcccgc ccgagatggc ggtgcgcatc
     1681 atcgcggacg tcgccgcggg tctgaacgcc gctcacgagc tgcgcgacgc gagcggcgcc
     1741 ttgcgcgggg tcgttcaccg ggacgtctct cctcacaaca tcctcatcgg cacgaacggc
     1801 gcgatcaagc tggtcgattt cggcgtcgcc aaagcgctcg gacgcgtgtc cgagtcgacg
     1861 cgcgcgggac agctcaaagg caagttcggc tacatgtcgc cggagcaggc gctcgggcgt
     1921 cccgtcgatc gccgcagcga cgtcttctcg ctcgggatcg tgctgttcga gctcaccacg
     1981 agccggcgcc tgtttcgagg tgagtccgac gtcgagacgc tgaagctcgt gatttccggc
     2041 caggtgccga gcccctccag catcgatccg agctacccgc cggagctcga gcgcatcgtc
     2101 atgcgcgccc tcgaacgcga tccccggctg cgctaccaaa ccgccgccga gttcgagcag
     2161 gatctacgcg gctttctgaa ggcggagcgc gtcgtggtcc ctcagagcgg cgtcgcgggc
     2221 ctgctcaagc gggtggtcgg ggagcgcatc gagcagcggc gacgtgcgat ccgggccgcg
     2281 ctcaagaaga taggcagcgg cggagcaggc ggcggcgacc tgctcacgac cgaaaccgcg
     2341 ttcacgccga ccggcaagga tcggatcagt gtcactggga ccgatccgtc gagcgcggga
     2401 atgggcgagt tcccggaccg acacctccga cgagctcagg atcgaagatc gccacggccc
     2461 caagacggcg tgaattggtc gagcgcgatc ggctacgcga tcggggtcgc ggggctcatc
     2521 atcgccatca tcgtcctgct ttttttgagc cgctgacctc cgcaagcggc cgtcgagcgc
     2581 gctcatggcg tgtgggaaac tcgcaccccg agcaacacgt tgagcttgag cccgcgacgc
     2641 ttttgtggtg tctcgtcaga cagaggacac tagcgcggtt gcgctccgcc gacttggcga
     2701 gccgacgggc cgattggcgc cgacggtcgc cgtcatggtc ggcccgagct aggatcagtg
     2761 ggttttttcg cgtccgcggc cgagggcgca gaggcagtcg agatacatgg gtaagattct
     2821 cgtcgtcgac gaccagcgga acatgcgcac gacgctcgcg atgatgttgc ggggcgcggg
     2881 gtacgaggtc gacgaagccg cagacggcgc gcagggccgt gaaagtggcg ccaccggcgc
     2941 ttacgacgtg gtcatcacgg atctgcgcat gggggagtac gacggcatcg atgtcttgcg
     3001 cgcggtgaag gaagcgcagc cgatgacgga agtcatcgtg atgaccgcgt atggaacgat
     3061 cgagagcgcc gtcgaggcca tgcgcgtcgg cgccttcgac tacatccaga aacccttcac
     3121 cgagcaagag ctcctcgtaa aggtctcgaa agcgctcgag agccggcgct tgaacgggca
     3181 agttcagctc ttcgcccagg acttcaaaga gcgcttccac gtcgacaacg tgatcggccg
     3241 ctcgcaggcg attcgagacg ttctcatgcg tctcatcaag ttcgctccga cggacgcgac
     3301 cgtgctcatc accggcgaga gcggcaccgg caaagagctc atcgcgcgcg ccgtccacgc
     3361 gaacagcaag cgcgctcagc gcccgttcgt gaccgtgaac tgtgccgcca tcagcgagac
     3421 tttgctcgag agcgagctct tcggtcacgc gcgcggggcg ttcacgggcg ctatcggcgc
     3481 gcgtaagggc ttgttcgaag aggccgacgg gggcaccttt ttcttcgacg aaatcgcgga
     3541 gacgacccct gtctttcagg ccaagctcct gcgcgcgatt caagaaggag agatccggcg
     3601 cgtcggtgag aacaagccga tccgcgtcga cattcgggtc gtcgctgcga ccaacgtcga
     3661 tctgtcgcga gccgtcgaag agcgcaggtt ccggcaagac ctttactacc gcctgaacgt
     3721 cgcgcgattt cagcttcctc cgctgcgcga acgtcgcgaa gacatcccgc tcctcgccga
     3781 gcatttcatc gcgaagtaca gccggaaaat gaccaagcgc gtccgtttcg ccgacggcgt
     3841 gatggagcgg ctcgtcgcgt acgactaccc gggcaacgtg cgcgagctcg agaacttggt
     3901 ggagcaatcg gtggcccttt gcaccggcag cgtcgtgcgc ctcgacgaca tcctgccgcg
     3961 ccccccgaaa tccgaagaaa aggcgcacgg acgtacgctt gccgacatcg tcgacgatgc
     4021 cgagcgcgaa gcggtcgagt cggcgttgcg cgcgttcgac ggcagccgcg agcgcgccgc
     4081 cgaggcgctc gacatcagcc cgacgacgct ctggcgcaag atgactcggc tcggcatctc
     4141 cttcgaagat cgctgaggtc ggctggggga ggccacggcg ccagcacgct tgcgcgcgtg
     4201 ctattagccc gccccgtgaa aaccgagctg ctcgactacc cgcttccgga cgccctcatc
     4261 gcgcggcacc caccgcacga gcgggacggg ggccggctgc tggtcctcga cggtgctcgc
     4321 cgaacccacc actcggttcg cgatttcgcg gagctcgtgc ccgagggcgc cctggtcgtg
     4381 ctgaacgcgt cgcgggtgcg gcgcgcgcgc gtgttcggcc aacgaaggcc ggggggaggg
     4441 cgcgtcgagc ttctcttcct cgagcaaagc ggccggagcg aggatgggcg cgagcgttgg
     4501 gccgcgattg gacgcgcgag caagcccctc cgccctggag tcgtgatcga agccgagggc
     4561 ctcgaggtgg aggtcgctgc caacgatgcg ggctcgctca cgctcttggc gcgagcgaac
     4621 gagccgctcg aaacgttgct cgatcgcatc gggcacgtgc cgctaccccc gtatctcggg
     4681 cgtggcgatg aacccgagga cgtcgagcgg taccaaaccg tctatgcgga gcgaaccggt
     4741 tccgtcgcgg cgcctaccgc gggcctgcac ttgaccgaag ccatgctcgc gcggatgcgc
     4801 gcgcgcggcg tcgacgtgca ccgtctggag cttgcggtcg gtatcggtac gtttcgcccg
     4861 gtgagcgccg aagatctcga cgcccacccc atgcacgaag agcggttctc ggtgagcgcg
     4921 gcgctcgttc gcgcggtggc ggagacgaaa gcgcgcggct tgccggtggt ggcggtcggg
     4981 acgaccgtgg tgcgcgcgct ggagagcgcg gcggcgagcg gcgagctcgt ggcttgcgaa
     5041 gagagcacgc ggctcttgat tcagcccggt taccgatttc gcgtggtcga ctcgttgctc
     5101 accaatttcc atcaaccgaa gagcacgctg ctcgcgctgg tcgcggcgtt tgccggctat
     5161 gacgccgtga tggcagccta cgccgaagcg atcgagcaaa agtaccggtt cctttcctac
     5221 ggcgacgcca tgttcattcc gcggagggct tcgtgatcac ttttcgcgtc gaggcaaggt
     5281 cgggcgtcgc gcgcgccggc gttctcacca cgccgcacgg agaggtgcgg acgccggcgt
     5341 tccaaccggt gggcacgcag gccaccgtga aggcgctgtc cgcggaggag gtcgcggcga
     5401 cgggcgcgcg cctcgtcatc atgaacacgt atcacctctg gcaacggccg ggccccgagc
     5461 tcgtcgccga gcgcggaggt ctccacgctt tctcgcgttg gccccactcg atcacgaccg
     5521 actccggcgg ttttcaggca ttttcgctgg cagagcggac gcgcgtcgac gaagacggct
     5581 tcgaattctc ctcgcacctc gacggtcgtc ggctgcgcct ctctcccgag gaggccatgc
     5641 gcatccaagg gctgctcggc tcggacatcg cgatgcagct cgacgtgtgc gcaccggccg
     5701 gagccgcgcg ccgcgagctc gagctcgccg tcgagcgcac gacgcgttgg gcggagcgct
     5761 gcctcgccgc gaagttcccg gagcaagcgc tgttcggcat cgtacaaggc gggctcgacg
     5821 tcgaactacg cctcgcgcac gcggaggcgc tcgcgaagct tccgctcgat ggtctcgcgc
     5881 tcggcggttt cagcgttggc gagccgatcg aagcgatgca cgagacgctc gcgcgcgtcg
     5941 cgccgacgct cgatgccacg cggccgcgct acttgatggg cgtcggcact ccgctcgaca
     6001 tcgtgcgggc gatctccgca ggcgtcgata tgttcgattg cgtgatgccg actcggaacg
     6061 cacgcaacgg tcaggcgttc gtgcccggcg ggaagctcgt catcaagaac gcgcgttacc
     6121 gaaacgaccc gcgccccctc gacgaagact gtacctgcga cgcatgccgc ggcggctact
     6181 cgcggagcta cctccggcac ctctacgtgg cgggtgagat tctggcgttg cgccttctca
     6241 cccaccacaa cattcaatat taccaatcgc tcgttgccgg ggcgcgcgcg gccatcctcg
     6301 cggaccgctt tggtgcgtgg gcagaaacga cactctccgc gttgtcgcac attcagcgcg
     6361 acgccgcgga gagcacggac accccagcag ggcgttgaaa aagccctgct attatgctga
     6421 ccggcaccac cgcgcggctc cgcatgaagt acccgcgtgg gggagggcgg cgcgtgcggg
     6481 cggcggttct cggcccgcgc ggcgccatcg cgcgttcggg ggagaggccg gtgagtaggc
     6541 gaggcgcgca aaccgcgcaa aacgcgccga gaggcggcgc tgggcgcata gcctggggcg
     6601 cggttcggct cgggcgcggc cgatgcttcg ccgtacgggc ggagctgaac gcactcggtc
     6661 gaagtgtcgt ggaccgcccc gcggaaggca tgccatgatg acggttggtc gccgacctcg
     6721 atgtccgaga agaagcaaat tggcaagatc ttgctccggc agcgcgcgtt gcgcgcggaa
     6781 gacttggatc gagccctcgg gactgaaggt cacggaaggc tcgcgtcgcg gctagctgcg
     6841 gaggggacga tctccgacgt cgccgcgctg aaggcgttgt ccgagcaaca cggcgtgccc
     6901 ggcatcgacc tctcgcagat ttgtattcgc ctggagcacc tcgatctctt gccgcgcgag
     6961 atcgccgaga agcatcgcat cttgccggtg ctcgagcgcg acgagcggct gttcgtcgcg
     7021 atggcggacc cgcgtgagcg cagcgtgatc gacgagctcg agttcgtgac cggcaagcgc
     7081 gtgtatccgt acgtcgcgct cgacgccgcg ctcgccaacg tgacggcgca ggcctacacc
     7141 cgcaaatcgc gtggggaatc tttttacgtc ggctcacatt gtccgcccga gctcttgcaa
     7201 aagctcggca tcgacgcgaa tggggagcca atcgcagaaa gcgtcgttcc gctcgagctg
     7261 atcgcgccct cgggagcccc gggatcgctc ggcgccgaag cggtcgtggt ggacgacgac
     7321 gtcgatcggg tctcgcgcga cgacgatatc gaggagcgag atttcggcga cgcctcgcgc
     7381 gagcacagcg tcgtgggacc gagcgacgcc gcggagctca accccgaccg cgcctcgaaa
     7441 gtaatcctgg tcgtcgacga cgaggccgag attcggcgaa tgttgacgca cttactgtca
     7501 cgcgccgggt acaaggtcat cgaggcagac aagggcgaac tcgctcttcg catggtccgc
     7561 gagcaggcgc cggatctcat cgtcctcgac gcgatgctgc ccgaggtgca cggcttcgag
     7621 atcgcgcggc gtatcaagaa gagcgaacgc tacggccaca ttcccatcat catggtgagc
     7681 gccgtttacc gcggctggcg ctacgccgaa gacgtgaagc gctcgttcgg tgtggatcac
     7741 ttcgtggaga agccgttccg catttcggag atcgttcaga gcgtcgagaa catgctcaag
     7801 gtgcgcgccg agtcggcgcc gcccccctcc gaccgcgatc tgtccgcggc cgcggaggcc
     7861 gcgttgaacg ccggcatcga ggcgtaccaa gcggggaaca tcgagcttgc gatcgagcac
     7921 ttgaagcgcg gcacgaccat cgaccccctg gcgttccgtc tgcacttcca cctggggctg
     7981 ctgtacggaa aacagggtca ggtcttcgac gcaatatcgg agctcgaggc ggccgtggag
     8041 agcaacggac agcactttgc tgctctcaag aacctcgcaa tcctctacca aaaagcgggg
     8101 ttcaggaacc gcgcggcgga gatgtgggag cgcgccctgc ctctcgctcc cgacgattcg
     8161 acgcgccaat cgatccgcca gcacttgctg aagctcctgt gagttcggcc gtcttgcatc
     8221 gcattcgtgc cccactgctc cgattcgaga cgaatgtgcc agcccggcgg ccgccccggc
     8281 tggccaatga gcccgggcga acatcgacgg catgcggacc cccaaatccg g
//
LOCUS       DASTKS010000404         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_64495,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000404 DASTKS010000000
VERSION     DASTKS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64495"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2191
                     /locus_tag="VFQ35_08395"
     CDS             <1..2191
                     /locus_tag="VFQ35_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9930693.1"
                     /translation="PVFHHESDVMLMVMHVRDPVRPLREIVPEIPPAVASVIEGCLAK
                     DPKARPSSARAMAEALAHALGVKLDVRGSGAYAATTAAAPADLVRGRIDLAAAHEGVP
                     DAGRSQVANPTAAHHAPVAPPVVAPPIATPPVPAPPAAPHAHGTPAANKPRSAPPPAP
                     PPERAVRTEAEAAAAPPAPTEARPVERLDAPLGATAESTLNKLMLRLKRQGDFPAFAQ
                     NVGEVSKKADADAAYSASQLGASILKDYALTAKLLRAVNSMYANRFGGKIYSIQHAIV
                     ILGFDRVRSLALSISLFKGTGKSGQSQRISDSAISSLVSGEIARNLAFDAKVSDEEAM
                     VCAMFRDLGRHLVVVYLPELYDQVMALAHAEHLSVRAASERTLGTTFARLGVAVAQTW
                     RLPPRLLTAIASVPSAGGPLTRAEDRLSSLAEFANDLCEIVASASSEVREREIAELLS
                     HHRHLLSIEQDKIPELLEAVQASFQERFSSLLGLDIKASRFLNNVAEVVKANATPGAS
                     PETAPSADAHPAAAHAAVAVPAHPAAAAPERVAVAPLELGRRLVEAPAPDDAAALEAR
                     LTELRRKLSARVPADVVLADALDACAQALGTSKLLVLVATPGRDAFVVRYGVRDDIDT
                     LAKELSFPLKLGRGLNDVFSSAYQTGRDARVEDALSPRMHATMPARYYEVIGSEAFAL
                     YPCVERGVPSALLFVDADSARSLPDPSRTPRLDPLRPLLAQAAALRS"
     gene            2339..2806
                     /locus_tag="VFQ35_08400"
     CDS             2339..2806
                     /locus_tag="VFQ35_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930694.1"
                     /translation="MTVDGKPPAFSKILLIEDNPDDVTLTLRALKKNAISNPVVVAND
                     GEEALDWLFCRGVYEERDQRDQPAIVLLDVKLPRVDGHEVLRAIRADPRTRLLRVVML
                     TTSREEQDIVESYSLGANSYIRKPVDFDEFIRVVGQIGLYWLLVNEPPPTHAT"
BASE COUNT          494 a         1038 c          943 g          472 t
ORIGIN      
        1 gccggtgttc caccacgagt ccgacgtgat gctgatggtg atgcacgtgc gcgatccggt
       61 gcgcccgcta cgcgagatcg tgcccgaaat tccaccggcc gtggcgagcg tgatcgaggg
      121 ctgcctcgcc aaagatccga aggcgcggcc gagctcggcg cgggcgatgg ccgaggcgct
      181 cgcgcacgct ttgggggtca aactcgacgt tcgcggctcc ggcgcttatg ccgccacgac
      241 cgccgccgct cccgccgacc tcgtccgggg tcgcatcgat ctcgccgcgg ctcacgaagg
      301 cgtgccggat gcaggtcgat cgcaggtggc caatccgacg gcggcgcacc atgctccagt
      361 cgcgccgccc gtcgtcgctc cgccgatcgc gaccccgcct gtcccggctc cacccgcggc
      421 gccgcacgct cacggcacgc ccgccgccaa caagcccagg agcgccccac cgcccgcgcc
      481 tccgccggag cgcgccgtgc gcacggaggc cgaggctgct gcggctcccc cggcaccaac
      541 cgaagcgcgt ccggtcgagc gtctcgatgc tccgctcgga gcgaccgcgg agtcgacgct
      601 caacaagctg atgctccggc tcaagcgcca aggcgacttt ccggcgttcg cccaaaacgt
      661 cggcgaagtc agcaagaagg ccgacgcaga cgcggcgtac tcggcgagcc aactcggtgc
      721 gtcgattttg aaagactacg ctctcaccgc gaagctactc cgcgcggtga actcgatgta
      781 tgcgaaccgc ttcggcggca agatctacag catccagcac gcgatcgtga tcttggggtt
      841 cgaccgggta cgctcgctcg ctctcagcat cagcctcttc aaagggaccg gcaagagcgg
      901 acagtcgcag cgcatctccg actcggccat cagctcgctc gtcagcggtg agatcgcgcg
      961 aaacctcgcc ttcgacgcca aggtgagcga cgaagaggcg atggtgtgcg ccatgttccg
     1021 cgatctgggg cggcacttgg tggtcgtcta tctccccgag ctctacgacc aggtgatggc
     1081 gctcgctcac gcggagcacc tcagcgttcg cgccgcttcc gaacgcactc tcgggacgac
     1141 cttcgcgcgg ctcggggtcg cagtggcgca aacctggcgc ctaccgccac ggctcctcac
     1201 cgcgatcgcc tcggtcccaa gcgccggagg gccgctcact cgcgccgaag atcgcttgag
     1261 cagcctcgcc gagtttgcca acgacctctg cgaaatcgtg gcgtctgcgt cttcggaagt
     1321 gcgagagcgc gagatcgcgg agctgctcag tcatcaccgg catctgctct cgatcgagca
     1381 agacaagatt ccggagctgc tcgaggccgt tcaagcatcg ttccaagagc gcttttcgag
     1441 cctgctcggc ctcgacatca aggccagccg ctttttgaac aacgtggcgg aggtcgtcaa
     1501 agcgaacgct acgcccgggg cctcgcccga gaccgcgccc tctgccgacg cgcacccggc
     1561 cgccgcccac gctgcggtcg cggtgcccgc gcaccccgcg gcggcggcgc cggagcgcgt
     1621 cgcggtggcg ccgctcgagc tcggtcgccg cttggtcgaa gcccccgccc ccgacgatgc
     1681 cgccgcgctc gaggcgcggc tcacggagct ccgtagaaag ctgagcgcgc gagttcccgc
     1741 cgacgtggtg ctcgccgacg cgctcgacgc ctgcgcccaa gcgctcggaa cgagcaagct
     1801 gctcgtgttg gtggccacgc cgggccgcga tgctttcgtc gtccgctacg gcgttcgaga
     1861 cgacatcgac acgctcgcga aggagctgag cttccctctg aagctcgggc gcgggttgaa
     1921 cgacgtgttt tcgtccgcgt accaaacggg ccgcgacgcg cgcgtcgaag atgcgctctc
     1981 gcctcgaatg cacgccacga tgccggcgcg ctactacgaa gtcatcggct cggaggcatt
     2041 cgcgctctac ccgtgcgtgg agagaggcgt cccgtcggcg ctgctcttcg tcgacgcgga
     2101 cagcgcgcgt tccctgcccg acccgtcgcg cacgccgcgg ctcgacccgt tgcgcccgct
     2161 cctcgcgcaa gcggcggcct tacgctcgta gacgacgcac cccggccggt tgttatcggg
     2221 aaagctgcgc tcctatcgga cgagtaccct ggacgggcga gccagacggc cgcggctggc
     2281 agaattcatg gtggggccga gtacgatcgt gtactttacc aagcagcgga gtcatcgaat
     2341 gaccgtcgac gggaagcccc ccgcattttc gaagatcctc ctcatcgagg acaatccgga
     2401 cgacgtcacg ctcacgctgc gcgctttgaa aaagaacgcg atttcgaacc cggtggtcgt
     2461 cgcgaacgac ggcgaagagg cgctcgattg gctcttctgt cggggcgttt acgaggaacg
     2521 tgaccagcgc gaccaaccgg cgatcgtgct gctcgacgtg aagctcccgc gcgtcgatgg
     2581 ccacgaggtt ctgcgcgcta tccgcgcgga tccgcgaacg cggcttcttc gcgtggtgat
     2641 gctcacgacg tctcgggaag aacaagacat cgtcgaaagc tacagcctcg gggcgaacag
     2701 ctatatccgc aagcccgtcg atttcgacga attcatccga gtcgttggcc aaatagggct
     2761 ctactggctc ctcgtcaacg aaccccctcc aacgcacgct acctgaaggc tgggcggcga
     2821 aagccatgtc tcgtccgctg cgagtcctct tgctcgaaga ttcagagagc gacgccgcgt
     2881 tgctcgcgct cgagctcaag cggggaggtt tcttgccgca gatgacgcga gtcgaagagg
     2941 aacaaac
//
LOCUS       DASTKS010000405         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1849098, whole genome shotgun sequence.
ACCESSION   DASTKS010000405 DASTKS010000000
VERSION     DASTKS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849098"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1223)
                     /locus_tag="VFQ35_08405"
     CDS             complement(285..1223)
                     /locus_tag="VFQ35_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9930695.1"
                     /translation="MSLDTIGSPPLWIGFVVFVLSMLALDLGVFHRKAHEVSLKEALG
                     WSLVWVALAAVFNLGLYIWAGPDKALEFATGYLIEKALAVDNIFVFVVIFAAFGVEAI
                     HQHRILFWGVLGALVMRALFIFAGAALLQRFHAAIYVFGALLAVTGVKLLLQREQEPS
                     PERNIVVRALRKIVPFASGPHGGRFFVKEHGRRVATTLLLALLAVEFTDVVFAVDSIP
                     AIFAVTRDPFIVFTSNIFAILGLRSLYFLLAGVITRFVYLKTGLALVLIFVGAKMLLV
                     EVVKVPIGLSLAIIALVLGGSIGLSLLRTSRADAPA"
     gene            1488..3674
                     /locus_tag="VFQ35_08410"
     CDS             1488..3674
                     /locus_tag="VFQ35_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9930696.1"
                     /translation="MRTPLLAIGFSWTLFGTGCGSSEPSLPEFDPPPASETPDARAAE
                     VISHMTRREKIELVHGGAARDWPVNPPPRGGNGYYQGNSRLHIPALYMADGSVGVGNR
                     TGPATALPSCMANAASWDLEQARQYGEVLGRELRAHRMNVNLGGNVNLSGREPRGGRT
                     FETKGEDPILAGKVTAAHLRAIQAENVIATVKHFALNDQETGRTLVDVLIDERSARET
                     DLLAFEVAIKDSDAQSVMCSYNLVNGQYACENADLLNGVLKTDWQFQGFVMSDWDATH
                     STATAANNGLDQEQPEDLYFGSSLDFAVQSGGVSEARLDEMVQRILRAMFAVGLFEVG
                     QSTVTIDEEAHASLAREFAEQGSVLLKNEGILPLDSAVGAVAIIGSHADMGVLSGGGS
                     AQVYPLGGAALREGSPSPPGWAEVIWDPSSPLAALKAKLPLAQLSFDAGVDPASAAAL
                     AAKAEVAIVFMSQWESEGMDVPRLNFTDLIHSSPIDQDAIVSAVARANPRTIVVLENG
                     GPKAMPWLEDVSAVLEAWYPGQRGGEAIANILVGDVNPSGKLPITFPAREGDLPRPTS
                     PTAIDLQKRVLDYGIEGVNVGYKWYEQQGIRPLFPFGYGLSYTTFSLENAELAVNRTE
                     GDLGLAVTCEVQNTGTRRGAEVAQVYVKLPESTGEGTRLIGWQKVWVEAGATRTASIS
                     VSGNDSSHPLSYWDVEAHAWRNAPGTYTVYLGNASDRLVKVGSFEL"
     gene            3757..4410
                     /locus_tag="VFQ35_08415"
     CDS             3757..4410
                     /locus_tag="VFQ35_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9930697.1"
                     /translation="MSKLRVNCFAVSLDGYGAGPEQSQENPLGRGGTRLHEWFYPTQT
                     FRTMVGQEGGTTDVDDEFARRGMENLGAWILGRNMFGPIRGPWPNEDWKGWWGDEPPY
                     HVPVFVLTHHARAPLAMAGGTTFHFVTGGILEALSRAKEAARGRDVRVGGGVATIRQY
                     LQAGLIDELHLALAPVLLGSGEHLFSGIDLPALGYSVRERVSSVAATHVLLSREGSR"
     gene            complement(4437..4847)
                     /locus_tag="VFQ35_08420"
     CDS             complement(4437..4847)
                     /locus_tag="VFQ35_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9930698.1"
                     /translation="MLRLDSPFVNGDARGMGTRAIAFLATRNADAARRFYEGTLGLEL
                     REETDFAIVFDAFGTMLRIQKTPDVKVAPYTAFGLEVDDIAQSLATLIEKGVEPRIYP
                     GLEQDSQGIWTAPSGARVAWFTDPDGHTVSLSQF"
     gene            4981..5181
                     /locus_tag="VFQ35_08425"
     CDS             4981..5181
                     /locus_tag="VFQ35_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930699.1"
                     /translation="MGAQAAHEQFEEHLLERERFMKGGRDTAIVVWLALLALVASFLL
                     VSRLAGETPLPPAGVGAQAPAR"
     gene            complement(5228..>5761)
                     /locus_tag="VFQ35_08430"
     CDS             complement(5228..>5761)
                     /locus_tag="VFQ35_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9930700.1"
                     /translation="RTELDKTFGRSAFDFLVNNAGVGVHASFLETTEEQFDEMVNVHL
                     KGTFFLSQKLAPLLADGGRIVNVSSGLARFTFPGYAAYAAMKGGVEVLTRYLAKELAA
                     RRISVNTLAPGAIATDFNGGFVRDNAEMNRLIASQIAFGRVGVADDIGGALAVLLAPE
                     SHWITGQRIEASGGMML"
BASE COUNT         1120 a         1819 c         1824 g          998 t
ORIGIN      
        1 gctgagcaca tcggggcgca acgaatgaag aacctgcgcg aagcgctgtt gtcgctgcag
       61 gacttccttg cgcaacgtgg cgcggagcgg tagcggctcg aaaacgcgaa gcaccagcga
      121 gcctcccctt cattcccaag cgagcgcccg cgaatcgagc caagcgatgc gttgcgatgg
      181 ccccgatgcg ctcggggggc tcgccagagc gaagcagagc cgagtctaca cgcgcacgct
      241 cgggggtgac ccggggcgtg cgcggcccac catcatcatt tgcatcaagc cggcgcgtca
      301 gcgcgcgaag tgcgcagcaa cgagaggcct atggaaccac ccaaaaccaa ggcgatgatc
      361 gcgagcgaga gcccgatcgg caccttcacg acctcgacga ggagcatctt ggctccaacg
      421 aaaatcagta ccaacgctag tcccgtcttc aggtagacga agcgcgtgat gacaccggcg
      481 agcaggaagt agagcgagcg caagccgagg atcgcgaaga tgttcgacgt gaagacgatg
      541 aacgggtcgc gcgtcactgc gaagatggcg gggatagaat ccacggcgaa caccacgtcg
      601 gtgaactcga ccgcaaggag cgcgaggagc agagtcgtgg cgacacgccg gccgtgctcc
      661 ttcacgaaga agcgacctcc atgcggcccg gacgcgaacg ggacgatctt gcgcagcgcc
      721 ctcactacga tgttccgctc gggggacggc tcctgttcac gctgcaggag cagcttgacc
      781 cccgtgacgg ccaagagcgc gccgaagacg taaattgccg cgtggaagcg ttggagcagc
      841 gcggcgccgg caaagatgaa caaggcccgc atcacgagcg caccgaggac gccccaaaac
      901 aggatccggt gctgatggat cgcttccacc ccgaatgcgg cgaagatgac gacgaacacg
      961 aagatgttgt ctaccgcgag cgctttctcg atgaggtagc cagtcgcgaa ctccagggcc
     1021 ttgtcgggcc ccgcccatat gtacaaaccc aggttgaata cggcggcgag cgctacccaa
     1081 acgaggctcc acccgagggc ctccttcagt gacacctcgt gagcttttcg gtgaaacacc
     1141 cctaggtcga gcgcgagcat cgacagaacg aagactacga agcctatcca gagcggtggc
     1201 gaaccaatcg tatcgagcga catgcagatc ctctcgcgcg ccgggacgcg catccgtttg
     1261 ttctgatgca cgcttgaatg tcggccatta cgaaatatct aaatgtcgtg tcgttatttt
     1321 ctacatttgc attttggcgc ggttttcgac gggacgcgct cacattcggc gcttctttta
     1381 ccccctcgaa atgattgatg tggggaggac attcccggtc ggcggatttc gtgggcgcgc
     1441 tcgcaacggg tatgcacaga ttcgggctca cgcgacatat gagcatcatg cgcacccctc
     1501 ttttggcgat tggcttttcc tggacgctct tcggaaccgg atgcgggagc tcggagcctt
     1561 ctctgcccga attcgaccca ccaccggcga gcgagacgcc ggacgcacgc gccgccgagg
     1621 tcatctcgca catgacccgg cgggagaaga ttgagctcgt gcacggtggg gccgcgaggg
     1681 actggcccgt caacccgcca ccgcgcgggg gcaatggcta ctaccagggc aactctcggc
     1741 tgcacatccc ggcgctctat atggccgacg ggagtgtcgg ggtcggcaat cggaccggcc
     1801 cggctacggc cctgccttcg tgcatggcga acgccgcgag ttgggatctg gagcaggctc
     1861 gccagtacgg cgaagtcctc ggtcgcgagc tccgcgcgca ccgcatgaac gtgaatttgg
     1921 gcgggaacgt gaacctcagc gggcgcgagc cgcgcggcgg gcgcaccttc gagaccaagg
     1981 gagaagatcc catcctcgcg ggcaaagtca cggccgcgca tttacgagcc attcaagccg
     2041 agaacgtcat cgccaccgtg aagcacttcg cgctcaacga tcaggagacg ggccgaacac
     2101 tcgtcgacgt gctgatcgac gagcgctcgg cgcgcgagac ggatctactc gcgttcgagg
     2161 tcgcaatcaa agactcggac gcgcagtccg tcatgtgttc gtacaatctc gtcaacggcc
     2221 aatacgcctg cgagaacgca gacctcctga acggcgttct gaagacggac tggcaattcc
     2281 agggattcgt catgtccgac tgggatgcga cgcactcgac cgccaccgct gcgaacaacg
     2341 gactcgatca agaacaaccc gaagacctct acttcggcag ctcactcgac ttcgcggttc
     2401 agagcggtgg cgtcagcgag gcgcgcctcg acgaaatggt gcagcgaatt ttgcgagcga
     2461 tgttcgcggt ggggctgttc gaggtcggtc agagcaccgt gaccatcgac gaggaagcgc
     2521 atgcatcgct cgcgcgagag ttcgccgagc agggctccgt gctgctgaag aacgaaggga
     2581 ttttgccgct cgattcggcg gtcggcgcgg tggcaatcat cgggtcgcat gcggacatgg
     2641 gcgtgctctc gggcggcggc tcggcgcaag tctaccccct gggcggggcg gcgcttcgcg
     2701 aaggctctcc ctcaccgcct ggctgggcgg aggttatctg ggacccgtcg tctcccctcg
     2761 ccgctctgaa ggcgaagcta cctctcgcgc agctctcttt cgacgcgggc gtggaccccg
     2821 cttccgctgc cgcgctcgca gctaaggcgg aggtcgcgat cgtcttcatg agtcagtggg
     2881 aaagcgaggg gatggacgtg ccgcggctga acttcacgga cctgattcac tcttcgccaa
     2941 tcgatcagga cgcaatcgtc agcgcagtag cgcgcgcgaa cccccgaacc atcgtcgtcc
     3001 tcgagaacgg cggcccgaag gcgatgccct ggctcgagga cgtgagcgcg gtgctcgaag
     3061 cgtggtatcc aggacaacgc gggggcgaag caatcgcaaa catcttggtt ggcgacgtga
     3121 acccctccgg caaactcccc atcacgtttc ccgcccgcga aggcgacttg cctcgtccga
     3181 cgagcccgac cgcaatcgac ttacaaaagc gtgttctcga ctacgggatc gagggcgtga
     3241 acgtcggcta caagtggtac gagcagcaag gtatcaggcc gctgttccca ttcgggtacg
     3301 ggctctcgta cacgacgttc tcactcgaaa acgccgaact cgcggtgaac cgcaccgaag
     3361 gcgaccttgg gctcgccgta acgtgcgagg tccagaacac gggcacgcga cgcggcgcgg
     3421 aggtggccca ggtgtacgtg aagctgccgg agtccaccgg cgagggcacc cgcttgatcg
     3481 gctggcagaa ggtttgggtg gaggccggag caactcgaac cgcttcgatc agcgtttcgg
     3541 ggaacgattc gtcgcatccc ctctcgtact gggacgtcga agcgcacgcc tggcgaaacg
     3601 ccccagggac gtacaccgtt tacctcggca atgcatcgga tcgcctggta aaagtcggga
     3661 gcttcgagct ttgaataaat tgttcgagcc ctgtcgatct gcgagcacgt cgttcgtcgc
     3721 ccttttagaa cgcacttcaa ccaggacgaa tggagcatga gcaaactacg cgttaactgc
     3781 ttcgccgtgt ctttggatgg ctacggcgcc ggtccagagc aaagccaaga gaaccctttg
     3841 ggccgcggag gcacgcgact tcacgagtgg ttctatccga cccagacatt ccgaaccatg
     3901 gtcggccagg agggcggcac caccgacgtc gacgacgagt tcgcgcgccg cgggatggaa
     3961 aacttgggcg cttggatttt aggtcgcaac atgttcggcc cgattcgagg cccctggcca
     4021 aacgaggact ggaaaggctg gtggggggac gagccgccgt accacgtgcc cgtcttcgtg
     4081 ctcacgcacc acgcgcgcgc gccgctcgcc atggcgggtg ggacgacctt tcacttcgtc
     4141 acgggtggga tcctcgaagc gttatcccga gcgaaggagg cggctcgcgg gagagacgtc
     4201 cgagtcggtg gcggcgtcgc cacgattcgt cagtacctcc aagcagggtt gatcgacgag
     4261 ttgcacttgg ccctcgcgcc cgtcttgctc ggctctggag agcacctgtt cagcggaatc
     4321 gacctgccgg cgctcggcta ttccgttcgt gagcgcgtgt cctccgttgc cgccacacac
     4381 gtgctcttgt ctcgggaagg ctcccgctag tcccggctac cgtctcgctg agtcggctaa
     4441 aactgactca gcgagaccgt gtgaccgtcg ggatccgtaa accaagccac gcgcgcgccg
     4501 ctcggagccg tccaaatccc ctgcgagtct tgctccaacc ctgggtaaat gcgcggctcg
     4561 acgcccttct cgatgagagt cgcgagcgat tgcgcgatgt cgtctacctc gagaccaaaa
     4621 gcggtgtacg gcgccacctt gacgtcgggg gtcttctgaa tccgtaacat ggtgccgaac
     4681 gcatcgaaga cgatcgcgaa atccgtctcc tcgcgtagct cgagcccgag tgtgccctcg
     4741 tagaacctcc gcgccgcgtc ggcgttcctg gtcgcgagaa aagcgattgc cctggtaccc
     4801 atgccgcgag catcgccgtt cacgaatggg ctgtccagcc gtaacacttt cagcccgact
     4861 cacgtctcga ctccgctgct cctcctgagg actcgagaca gctcggcggc gtgctcgtgc
     4921 tcgcgtatac tcgagcggtc ggacgtgaga agtctgtccg accaagagga ggaatttgcc
     4981 atgggagctc aagcagccca cgagcagttc gaagagcatt tgctcgaacg cgagcggttc
     5041 atgaagggag ggcgggacac ggcgatcgtc gtgtggctcg cgctcctcgc gctagtcgct
     5101 tcgtttctgc tggtctcgcg cttggccgga gagaccccgc tccctccagc cggcgtcgga
     5161 gcacaagcgc cggctagatg actctcggct cgctcgcgac tgactcaccc attcgcgggc
     5221 cctcagctca gagcatcatg cctccggatg cctcgatgcg ctgccccgtg atccaatggc
     5281 tctcgggcgc cagcaaaacc gcgagtgcgc ctccgatatc gtccgccacg ccaacgcggc
     5341 cgaacgcgat ttgcgatgcg atgagccggt tcatttcggc gttgtcgcgc acgaacccac
     5401 cgttgaagtc ggtcgcaatc gctcccggcg cgagcgtatt gaccgaaatt cggcgcgcgg
     5461 cgagctcctt cgccagatat cgtgtcagca cctcgacgcc gcccttcatg gccgcgtacg
     5521 cggcgtagcc cggaaatgtg aagcgcgcga ggccgctcga gacgttgacg atacgaccac
     5581 cgtcggccaa caacggcgcg agtttctggg acaagaagaa cgtgcccttg aggtgaacgt
     5641 tcaccatttc gtcgaactgc tcttcggtcg tctccaagaa cgaggcgtgc acgccgacgc
     5701 ccgcgttgtt gacgaggaag tcgaaggcgc tgcgcccgaa ggtcttgtcg agctcggtgc
     5761 g
//
LOCUS       DASTKS010000406        10499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1403527, whole genome shotgun sequence.
ACCESSION   DASTKS010000406 DASTKS010000000
VERSION     DASTKS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403527"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..954)
                     /locus_tag="VFQ35_08435"
     CDS             complement(265..954)
                     /locus_tag="VFQ35_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930701.1"
                     /translation="MRSGRWALLLVTGLSGCARTRAVVFQGHESAARVSPEQVVEASV
                     MPRSATWLGRVAARCQPFEPKQSFEDRPLFDLDCSEARLRSLLREGAAEAGGNLIAEV
                     VCNSGSSRECRALVATLPAALAGLPIEPPLRAAVGNARVAFLLESRDAPPPARPSAVV
                     SEQPLPLPSHTPFGCVRVRCEDCSEVDARDALHVAAARLGAADLVGPHCRTFADHSEC
                     AGELVRTERAD"
     gene            complement(1123..1518)
                     /locus_tag="VFQ35_08440"
     CDS             complement(1123..1518)
                     /locus_tag="VFQ35_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930702.1"
                     /translation="MKKLTSLLAMTAIAASVAVVGCGDDDDSTGATGGSAGSAGKGGA
                     AGSSSGGSTSKGGAAGGTSKGGAAGHAGAPAGGAGEGGVPTTTGGAAGEGGVPAQTGG
                     VPSTGGVGPMGGEGPGGTSPGGAGGSPTT"
     gene            complement(1867..2760)
                     /locus_tag="VFQ35_08445"
     CDS             complement(1867..2760)
                     /locus_tag="VFQ35_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HET9930703.1"
                     /translation="MTHERLDREGSPVSLASVQIDVTTRLGAPSPVTTTGFPSLDNFL
                     GGGLRPGVLVTVVSAPGLGRSTFALFMAYMAARSRASTLFTAVGLDDTEIVARLAARA
                     LHRERPEIAAAYGAIWSGRALTQPEVRAPLLGAIETVVRKVGSSLHLKRAGSLEATGV
                     LAESASELWTRSERVVVVVDDLEAYYASSDGNPAKQASVNASLAGRVMQVAFDLKSVA
                     EQGCAVVATALSCHAELVMPASSLTLELRASSREATGLPSELSLGARELDLVVLKNRL
                     GEAGSIPIRMVPGAGFVEERR"
     gene            3341..4714
                     /locus_tag="VFQ35_08450"
     CDS             3341..4714
                     /locus_tag="VFQ35_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930704.1"
                     /translation="MPKSSGSGGSSFTSKSSLKAGAERFLSQVVAHSLASGIRTTEDF
                     LQAFPPLDIMRGLDPVPDLRGAILTKAVGFHERIARKKTAQSAAEDLRIALEEGVATA
                     ADILSLLSDDDRVRYLDRPRLFAFAIDAGFFAGSPPTQAALDAMVFLLETALGEQVLT
                     LADIADGVTFESISRLLPPAELQRVVLAALEHGRSGQPLTEDRLLDVVPLPTLVRHIP
                     LDAFWSQVILAKVAVPAGWLAAGSVPPPSQRQRSEPIKAPSPPVEAPQAKPSPAPAPK
                     ASKAQARPNNGKPAAPASAESEPPPAEGSGEEDVARKRVLDRLSAIGRLPTRHPDLST
                     QILFSIDSMYAELMVAPSDDEREEAIRDAFPNEQHMATALLALIELLEPSIDVNDPEI
                     AKADVDSLINVFLIEERDHRERAASQRPPVAPSAAPAPPQRRSAPPPPLPRNPPPPPV
                     PNAEKAR"
     gene            4834..5232
                     /locus_tag="VFQ35_08455"
     CDS             4834..5232
                     /locus_tag="VFQ35_08455"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9930705.1"
                     /translation="MASLERETWTQAPADEVWDAIRDVGALHTRLVPGFVIDTKLEPG
                     ARVVTFGNGKVVKEPIVTLDDARMRLVWTAQGGSLTHYNGVLRVFSERGGSRVVWTAD
                     FLPNEAEAALTGMLEEGLRVMKATLDGRSA"
     gene            complement(5243..6088)
                     /locus_tag="VFQ35_08460"
     CDS             complement(5243..6088)
                     /locus_tag="VFQ35_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9930706.1"
                     /translation="MATRILAEYRALTAIDFRCEHRKHERPFTEVHAGHSISYVRQGS
                     FGYSSRGRAHLPVAGSLVVGFPGDEFVCSHEHATGDECLWFRLEPALVDSLGGDAAVW
                     RVGVVPPLSELVVLGELGQAIADGQCDLGFDEAAMMLTARFIEVVAGQRRARECSPVE
                     RRRAVEAALFIDAHCTEELSLEDTARHARISPFHFLRTFGRAFGVTPHQYLVRSRLRR
                     AARLLPDASYSIASIALEVGFNDISNFVRTFRRAAGLSPSAFRRLARRERDGLGERLV
                     LAPRD"
     gene            6324..6407
                     /locus_tag="VFQ35_08465"
     tRNA            6324..6407
                     /locus_tag="VFQ35_08465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6358..6360,aa:Leu,seq:cag)
     gene            7899..8507
                     /locus_tag="VFQ35_08470"
     CDS             7899..8507
                     /locus_tag="VFQ35_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930707.1"
                     /translation="MAIEVPSVRILIVDDDRAICEYMQTLLEKDGFVVKTLSDPTLVE
                     DEVRQGDYHVMILDLMMPKLDGIEVLKRIRSIDSDIAVIIFTAHPNLETAIASMKLDA
                     VDYIKKPFNVDEFREVLSRVMKKKGLARTPEEQLHKIIGDTIRTLRKDKELTLKQMSR
                     RTGLSISLLSQIERAESSPSISSLYKIAIALDSKIHDLFGNN"
     gene            complement(9656..9729)
                     /locus_tag="VFQ35_08475"
     tRNA            complement(9656..9729)
                     /locus_tag="VFQ35_08475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9693..9695),aa:Met,seq:cat)
     gene            complement(9899..>10499)
                     /locus_tag="VFQ35_08480"
     CDS             complement(9899..>10499)
                     /locus_tag="VFQ35_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9930708.1"
                     /translation="ILARPSVPATRAAVPAPQRDRPNDTTDSDQRVALRVASLLQAST
                     LGGDASEGPGGEAGPGLVGAGEGNASGARAQSAGSGSGLGEPTADPALDPFYRGLRAR
                     VERALEDTFPQWAKLEGRGGLVVFEFTLDGAGRIANVSVVRPSGIAEFDRNVVLAVRQ
                     LPAFGRIPAGLASRAIRFTFDEQNPAVGRRGPGPGHVAD"
BASE COUNT         1969 a         3535 c         3252 g         1743 t
ORIGIN      
        1 cccccccgcc gcatcgcccg cctggaatgc gagctctcgg tgccgacagc cacggcgcag
       61 gcattgagcc cggaggctcg cgcagagctc gagcacacgg cgcacacgtg cccggtgcgc
      121 ttgagcctgc tcgacgcgct cgaggtcccg gtccgcttcg cctggggcga ctagcgagac
      181 gggggcacag ggccttcgct gccctgctgg cagccaagat cggcgcaggc tcggccgagt
      241 cgctccgcta tcgctcgcgc ctgctcaatc agcgcgctcg gttcgaacga gctctcccgc
      301 gcattcggag tgatcggcga atgtccggca atgcggcccg accaagtcgg ctgccccgag
      361 ccgcgccgcc gcaacgtgga gcgcgtcgcg agcatcgact tctgaacagt cctcgcagcg
      421 cacgcgcaca caaccgaatg gcgtatggct cggaagtggc agcggttgtt cgctgacgac
      481 ggcgctcggc cgtgccggcg ggggtgcgtc gcgcgactcg agcaggaacg cgacgcgcgc
      541 gttgccgacc gcggcgcgca gcggtggctc gatcggcaaa cccgctagag ccgccgggag
      601 cgtcgcgacg agcgcgcgac attcacggct cgagcccgaa ttgcagacca cctccgcgat
      661 caggttcccg cccgcctcgg ccgcaccttc gcgcaacaac gagcgcagcc gtgcttcgct
      721 gcaatctagg tcgaagagcg gccgatcttc gaagctttgc ttcggctcga atggctgaca
      781 ccgggcggcc acgcgcccca gccacgtcgc cgaacgcggc atcacgctcg cctcgacgac
      841 ctgttcgggg gagactcgcg ccgcgctctc gtgcccttga aagacgactg cgcgtgtgcg
      901 cgcacagccg gatagcccgg ttaccagcaa gagcgcccag cgcccgctcc tcatctctcc
      961 accatcgcgc agagatctcg gcgaggcaac ggcaagcgcg ctcggacccc gatgcccgaa
     1021 aaagaaaacg gcatagcgga gctaccgcta tgccgttttg gaagctgatg cttcgaacga
     1081 atcagccgtc cccgggaatc aggctgactc gcgatcgagc gatcaggtcg tgggcgaacc
     1141 gccagcgccg ccggggctcg tgccgccggg gccttcgccg cccatcgggc caacgccacc
     1201 cgtgctcgga acgccgccgg tctgagccgg gacgccacct tcacccgcag cgccgccagt
     1261 cgtggtaggc actccgccct caccagcgcc acccgcagga gcacctgcgt ggccggctgc
     1321 tccgcccttg gacgtgccgc cagcagcgcc acccttggac gtggagccgc cgctgctcga
     1381 gcctgccgct ccgcccttgc cagcgctgcc ggccgatccg ccggtcgcgc cggtgctgtc
     1441 gtcatcgtcg ccgcaaccga cgaccgcaac gctggccgcg atagcagtca tcgcgagcaa
     1501 gctagttagc ttcttcatga tctgatcctc cgaaataacc taggttatgc tgggcccagg
     1561 gggccgaggc gccgtgagca ctttccgtgc cggacgaagc accctgatcc ccaattctat
     1621 ttcaagtatc cgagacgatt aaggaattca gtgctccgta gccagccgtg ctgagcgccc
     1681 gaacagcttg agccgagact ttcctgtcat acgtgtgggg gcttctacga tgagagccga
     1741 cagattgcaa tcccatgcga gataatttcg gccgaccgaa tcagaagcgc tttcggttac
     1801 ttaagtgacc gaaggtgacg ttccatccgc gccgggtggg cgtgcccgta caaagccgct
     1861 gcgttttcag cgtcgttctt cgacgaagcc ggcgcccgga accatgcgga tcggaatcga
     1921 gcccgcctcc ccgagacggt tcttcagtac gacgaggtcc agttcacgcg cgccgaggct
     1981 gagctcactc ggcaaccccg tcgcctcgcg ggaagaagct cggagctcga gcgtgaggct
     2041 cgatgcgggc atgacgagct ctgcgtgaca gctcagcgcg gtcgcaacca cggcgcagcc
     2101 ttgctccgcg acgctcttga ggtcgaacgc cacctgcatc acgcgcccag ccaaagaagc
     2161 gttgacgctc gcctgcttgg ccgggttccc atcggaactc gcgtagtacg cctcgaggtc
     2221 gtcgacgacg accaccacac gctcggagcg cgtccagagc tccgacgcgg actcggcgag
     2281 cacgcccgta gcttcgaggc tacccgcgcg cttgagatga agcgacgaac ccacctttcg
     2341 caccacggtc tcgattgcac cgagcagtgg cgcgcgcacc tcgggctgcg tgagcgcacg
     2401 cccagaccag attgcgccat aagccgcggc gatctccggg cgctcccggt gcagagcgcg
     2461 cgcggcgagg cgtgcgacaa tctcggtgtc gtccagccca acagcggtga agagggtgct
     2521 cgcgcgcgag cgcgccgcca tgtacgccat gaagagcgcg aacgtcgagc gaccgagccc
     2581 gggcgcagac acgaccgtaa cgagaacgcc gggacgcaga ccgcccccga gaaagttgtc
     2641 gagactcgga aagcccgtcg tcgtcaccgg agacggcgcg ccgagccgcg tcgtcacgtc
     2701 gatctgtacg ctcgccaacg aaaccggcga accctctctg tccaagcgct cgtgagtcac
     2761 gcaccgagtc tacgccggaa ggcggcgcga aacggaaggc cttcgcgaga cgcgcggaga
     2821 gcccctcaat gggcgtatca aacctggaag cacgtatgat ttcgatcgtt ccgcgagttc
     2881 cgaagcgaga cgagctctcg tgactattcc gtgatttcgt tgaaaaatcg ccttggcacg
     2941 cggctcgcac atcgaccggg taaaatgtcc cgccgtcgcg ctgaccaggc cccattgggc
     3001 ttcacatcga atggtattct gctcgtcaac gtgctctcgc agctgagcaa agaacacgct
     3061 cgagctcttc gcaaagcact cccgtccaag tttggaaccg actggttcat cggcgcagcc
     3121 gtcgaccaga cgcgaacacc agccgctctc gctcgcttgg acgacgcggc cgcagaagcc
     3181 gctgcgcggc tcgttgcatc cgaggcaaag tcgcccgtaa aaaagcgagg cccgcgaagg
     3241 tgagtctgaa atttccgtca gaacgagaat aaaatcacgt actcgcgcgc cgctgcccgt
     3301 tgttgcgaga cctaccgacg catacgatgt gccactcgtc atgccgaagt cgtcagggtc
     3361 cggtgggtcg tcgttcacca gcaagagcag cctgaaagct ggcgctgagc ggttcctttc
     3421 acaggtggtt gcgcactcgc tcgcgagcgg catccgtacc acggaggact tcctgcaggc
     3481 gttccctccg ctcgacatca tgcgcgggtt ggaccccgtg cccgatctcc gcggcgcgat
     3541 cctgacgaaa gccgtcggat ttcacgagcg catcgcccga aagaagactg cgcagtccgc
     3601 cgcagaagat cttcgcatcg cgctcgagga aggcgtcgcc acggccgccg acatcttgtc
     3661 gctgctctcg gacgacgacc gcgttcgata cctcgatcgt cctcgattgt tcgccttcgc
     3721 catcgatgct gggttctttg ccggctcccc tccgacccag gccgcactcg atgccatggt
     3781 tttcctgctc gagacagcgc tgggcgaaca agtgctcacg ctcgccgaca tcgcggacgg
     3841 cgtcaccttc gagagcattt cgcgcttgct gccgccggcc gagttacagc gtgtcgtgct
     3901 agcagccctg gaacacggtc gtagcggcca gcctctcacc gaagaccgat tgctggacgt
     3961 cgtgcctctc ccgacgctcg ttcggcacat cccgctcgac gctttctggt cgcaggtgat
     4021 tctggcgaag gtcgcggtgc ccgcgggttg gctcgcagca ggttcggtac cgccaccgag
     4081 ccaacgacag cggagtgagc ccatcaaagc gccatcaccg cctgtggaag cgccccaagc
     4141 gaagccatcg cctgcccctg ctcccaaggc cagcaaagcc caagcgcgac ccaacaatgg
     4201 taagccggcc gcgccggcaa gtgccgaatc ggagcctccg cccgccgagg gctcgggaga
     4261 agaagacgtc gcgagaaagc gggtgctcga tcggctttcc gcgatcggcc gccttccgac
     4321 acgacacccc gacctgtcga cgcaaatctt gttctcgatc gattcgatgt acgccgagct
     4381 catggtggcg cccagcgatg acgagcgcga agaagcgatt cgcgatgcct tcccgaacga
     4441 gcagcacatg gcgaccgcgt tgcttgcgct catcgagcta ctcgagccga gcatcgacgt
     4501 gaacgatccg gagattgcaa aggccgacgt cgactcgctc atcaacgttt tcctcatcga
     4561 ggaaagggac catcgcgagc gcgctgcctc gcagcgcccg ccggtagccc cgagtgctgc
     4621 gccggctccg ccccagcgac gttctgcacc gccgcctccc ttgccccgaa acccgcctcc
     4681 gccgcccgtt ccgaacgcag aaaaagcgcg ttgaagtcgg ctcgcggccg ttgctactcg
     4741 agagccaatt gcagccctcg gttcagttcg tagcaagctt ttccatgtga gcttcgggtg
     4801 gcgcttctac cctccgaggg aaacggagga acgatggcat cactcgagcg agagacgtgg
     4861 actcaggcac ccgccgacga ggtttgggac gcaattcgcg acgtgggggc gctgcatacg
     4921 cggctggttc cgggattcgt gatcgacacc aaactcgagc ccggagcgcg ggtggttacg
     4981 tttggaaacg gcaaggtcgt caaagagccg atcgtcactc tggacgacgc tcgcatgagg
     5041 ctcgtctgga ccgctcaggg aggctcactg acgcactaca acggtgtcct gcgcgtcttc
     5101 tccgagcgcg gtggcagtcg cgtggtgtgg acagccgact ttcttccgaa cgaagccgag
     5161 gccgcgttga ccggcatgct cgaagaaggg ctccgggtga tgaaagcaac cctggatggg
     5221 cgctccgcgt aagccaagct cgtcagtcgc gcggcgcgag cactaggcgc tctcccaacc
     5281 catcgcgctc tcgccgagcc aagcgtcgga aggcgctcgg cgaaagcccc gcggctcgac
     5341 gaaatgttcg cacgaagttc gagatgtcgt tgaagccgac ttcgagcgca atcgaggcga
     5401 tcgaatacga agcatccggc aagagccggg ctgcgcggcg caaccgcgaa cgcaccaggt
     5461 attgatgtgg cgtcacgccg aaagctcgac cgaaggtcct gagaaaatgg aacgggctta
     5521 ttcgtgcgtg gcgcgccgtg tcctcgagcg agagctcctc ggtacaatgc gcgtcgatga
     5581 agagcgcggc ctccaccgcg cgccgccgct ccaccggcga gcattcgcgg gcccgccgct
     5641 gccccgccac cacttcgatg aagcgagcgg tcagcatcat ggctgcttcg tcgaaaccga
     5701 ggtcgcattg gccgtcggcg atggcttgcc cgagctcccc gagcaccacg agttcggaca
     5761 acggcggcac cacgccgacg cgccaaaccg cagcgtctcc accgagtgaa tccacgagcg
     5821 ccggttcgag gcgaaaccag aggcactcgt ctcccgtcgc atgctcgtgc gagcagacga
     5881 actcatcccc cggaaacccg acgacgagcg agccagctac cggtaggtgt gcgcgaccgc
     5941 gagacgagta gccaaagctt ccttggcgca cgtacgaaat cgagtgcccc gcgtgcactt
     6001 cggtgaacgg gcgctcgtgc tttcggtgct cacaacgaaa gtcgatcgca gtcagagctc
     6061 gatactcggc aagaatgcga gtcgccaccc agtggccgat agcgctccgc ctcgctcggt
     6121 gcttggcaaa tcttgctcct cagaccgggc ggaggctcgg ccagcacgcc ggccacgagt
     6181 ccgaacagac gtcatgtgag cccagcccaa cttcgtcgcc cacttcgagc gagagggctt
     6241 gcacaggcct ccccggctga ggtagaccgc cctcgcttcg ttcatcctcg aattcagcga
     6301 actctgggat ggcgagacac ctcgcgaagg tggcggaatt ggcagacgcg ctggattcag
     6361 gttccagtgg ggttactcct gtgagggttc aagtccctcc cttcgcaccg aaagatcgag
     6421 ctcgctgctg ccactcggca ctctacgagc gcgctcgtta gcccgcgtcg gttctctccg
     6481 tggagaggcg ctgagctgag gcaaggtccg cgttcgcgtc ggacgagcgg gcgccgacac
     6541 ccggcgccgc cgcagccctc gccaaaagac tctgcttcgt cggcgagact cgcgattcgc
     6601 cctcccaccg cagcacttcc cgagggcgca ctcgagaagc acatgtgcgc gactcggagg
     6661 cttgatgcgt aggccgccca ccaatctttg gaccagtact ttacgctgcg aactgagcag
     6721 aattacagtt ggtgcatggg tcagtccgga cgaaagctga tgttggcgct cgtcgcgttc
     6781 ggcgccggtg gatttatggg actggttgca gcggcagccg acgacacggg caacgttgcc
     6841 ggcgtcaggc gttcagcaac ccgcccggat gtcgtagccc ccgccaggct gaacgcactt
     6901 cccctgttgc cggctcccgc cgaggctcaa agtcagcttg ccgccgatcc ccccgagctc
     6961 cgccaggcag tggcttcgag tgaatccatc gcgacccaag tcagcacgcg tcgcgttcgg
     7021 gagtcgcaac cgaagcccgc cagtctgccg cgaaagacag cacagcacgt gagcaagtcg
     7081 ggctcctgga cacggcgttc cgagatcatc gatccttggt caacccgccg ttgacgttgg
     7141 gaagagcgac gtgaccgcgc tctcgcgcgg ctctgcaaat ccaaccaaca cgccgcgcgc
     7201 cgactccaac ggactccgct actcggccgc agaaggcgcg tcgctcgatg caggaatggc
     7261 tccgacggtc gcgattgctt cgcaacgcgc gtcgccgagt gccaacgcgc gcctcgaaag
     7321 acgggattgt cgcgtacgca gactcgtgca gagacgtcct tgcgtgacac agaagctcga
     7381 agcgcttcgt cgtgggtaac ggccgtgaga cgcgaaacgt tcgacgcacg cgtgcaaatt
     7441 ggaaacgcaa atgcgcactc gtacgtcgcc gctctgctca acacgaaaga gccacggcat
     7501 tctcgcttcg cgcactgtcg cggagcgtcc ggcgatttcg cgcgagaatg gacgctcggc
     7561 acgctgatct ttgcgagatt gcgtgcgaag gcctcgacgc agatcacgtc cggttggaat
     7621 ttgggtgcct gtgcgtgtgg gcccggctac tcacagtgaa atcctcgcga ccgtggggca
     7681 cagactcggg gcgcggaaac ttgcgacacg aagcgcaacc cagctcccag atttgctcgg
     7741 aatccgccat ttatcggccc gcgagacaac aaatttcgct tttatttccg atgtttacaa
     7801 ttacgaaagt caagggcaaa aatctagtgg tgtcgtaacc cggtaggctt tatagtgtca
     7861 ctgaaagacg cagagacctg tctgcaaagg agcacacctt ggcgatcgaa gtaccgagcg
     7921 ttcgaatcct gatcgtcgac gatgaccgag cgatctgcga gtacatgcag acgctgctcg
     7981 agaaagacgg cttcgtcgta aagacattga gcgatccgac gctcgtcgaa gacgaggttc
     8041 gtcagggtga ttaccacgtc atgatcctgg acctcatgat gccgaagctc gatggcatcg
     8101 aggtactgaa gcggatccgg tccatcgaca gcgacatcgc ggtcatcatc ttcacggctc
     8161 acccgaacct cgagaccgcg atcgcttcga tgaagctcga cgccgtcgac tacatcaaga
     8221 agccgttcaa cgtcgacgag ttccgagaag tgctctcgcg cgtgatgaag aagaagggcc
     8281 tcgcgcgcac gcccgaagag cagctgcaca agatcatcgg agatacgatc cgaacgctgc
     8341 gcaaggacaa ggagctcacg ctcaagcaga tgagccgacg cacggggctc agcatctcgc
     8401 tcctttcgca gatcgaacga gcagaatcga gcccgtcgat ctcgagtctc tacaagatcg
     8461 cgatcgcgct cgactcgaag atccacgacc tcttcggcaa caactgagca cgccgatccc
     8521 gcgcgttcag cgcggtctac cgaacgcggc tgccccggtc gagctcgcag cgcaccgagc
     8581 tcgacccgtt ccccgggcaa acctgaaccg tcgttcaccc aagagggatg cgtgagaatc
     8641 tcgccttttc gcgcttccgt cccatgacga ctgccgagcg tgggggcttc tcgcgatagc
     8701 ggattgctgg tgcaccgcag ccgagaaaca gacgcgccga gacgctggga atgacgctga
     8761 aggccactaa ccaagggccg agacgcggga gttgcgtgcg ttcggcaacc cgcgcgcgca
     8821 cgaatcatgt gcggcaaggc ggcagcgcga tgccgtcagg cggtacgcgc cgcaggatgc
     8881 ttagccggag gccaacggcc ggtccgtgcg gagtcgccga tgacgaaccc gagtcgttga
     8941 aagaattacc atggtgccgg cggccctaac gggtcactct cgagcccgca gtgcgcgcca
     9001 ccatcaagca ccaccacact cgcatgcgct ctgcgtgccg cctcggcatt gccacgtagc
     9061 cgagtcttac tcgcaacgtg agggcaatca gctgggcttg ttaacgcagg gcccgcaccg
     9121 caggcggggg gttgtttggg ggggtgtgta tggggggcaa accgcggtac ggaccctgca
     9181 ccgttactct taccacgcgc tcgctcgttc cgcgaacgaa aacaagaaat ctcgcggcac
     9241 tcggccattc gaccgagccc cgatctgtgg gagtttcttc gtagtgaagg cctgcagggg
     9301 cgccgtaagc ctgctcgccg tcacaagcgt tactccatca tgccgcgctc ttttggcgcc
     9361 ctttgtgggg tgtgcttcgg acttagtcgg ggactcggga ttccagcgtc gtttactggt
     9421 caagacgacg tgaccagaac tcgaaactgc gcgcctgtct gcgatgctgc gcaatgcgct
     9481 gtgtggcgcc ttggtgcctg ttgattgctc actgccccaa gccatgcagc gagaaagccc
     9541 tcctcggggg ggcacatgcc gatacgcggc gggtagtgcc ggatgcggac agcaggcagc
     9601 ccgcagcgaa ctggagcggc agcagacgcc gccgcctgag accaggtttt cttcgtgccg
     9661 aaggtcggac ttgaaccgac gaccccacgc ttatgaagcg cgtgctctaa ccagctgagc
     9721 tacttcggcg agctccggcg ccgcaatatt atcttcggga aatctcgtca agtcggccac
     9781 gcaacctggc gcaaaaatcg cgctcggcac gctggacgcg gcccttctcg cggtcggaca
     9841 gccctcccgg gggacccaag tttctccgcc ctgccgcgag gcaggcttgc gagattcctt
     9901 agtcggcgac gtgcccggga cccggtcctc gtcgccccac cgccgggttc tgttcgtcga
     9961 aggtgaagcg aatcgcgcgt gacgcgaggc cggccgggat tcgcccaaac gcgggaagct
    10021 gccgcaccgc gagcactacg ttccggtcga attccgcgat accgctgggc cgcactacgg
    10081 aaacgttggc gatgcgacca gcgccgtcca aagtgaactc gaatacgact agcccgccac
    10141 gtccctcgag ctttgcccac tgagggaacg tatcttcgag cgcgcgttcg acccgcgcgc
    10201 gaagtccacg atagaaaggg tccagagctg ggtcggcggt cggctccccg aggccgctcc
    10261 ccgagcccgc agattgcgct cgtgccccgc tcgcattgcc ttctccagcg cccactaggc
    10321 ccggccctgc ctcgccgcca gggccctcgc tcgcgtcgcc gcccagtgtg ctcgcctgca
    10381 gcaaggacgc gacgcgcaac gcgactcgct gatcgctatc cgtcgtgtca ttcgggcgat
    10441 ctcgttgcgg cgccgggacc gcggcgcgcg tcgccggcac cgacgggcgt gcgagaatg
//
LOCUS       DASTKS010000407         8093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1403668, whole genome shotgun sequence.
ACCESSION   DASTKS010000407 DASTKS010000000
VERSION     DASTKS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403668"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFQ35_08485"
     CDS             <1..766
                     /locus_tag="VFQ35_08485"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HET9930709.1"
                     /translation="GNEDHYSWSFGVGVERVARASLMLRYRLLPYLYAAFLEASETGA
                     PVQRPLVFDFQDDIESRRIDDQFLLGSSLLVAPVLKAGQTARHVYLPPGDWVDFHSGE
                     RHAGESYVTLPAPLGKIPLLVRAGAVIPMLERAPDSTHGLAPELIELHVFAPGRDGSY
                     ESLLYEDDGVSRAHERGAHLRTRLTLVRSGATLLLRAAVSGQSFPQFQRRRLRVVLHA
                     ETPLEATLDTRPLSVDASNVVFDDRGGGFELRITLA"
     gene            complement(768..1709)
                     /locus_tag="VFQ35_08490"
     CDS             complement(768..1709)
                     /locus_tag="VFQ35_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930710.1"
                     /translation="MKRWLERAARIIGASVMVCIAPVRAYASVALCVDIKASDAERAG
                     FEKLVRSELARHPSHRVGETACDSRLEAELFWLGKSRYLTLRIDQEVPLRYTLQSDRD
                     LEIRLSEGISRVLNSDPAYLAEDPSRLSAGERATRAVLVHGSNSYRLAFFEGMARTDN
                     GLAFAPGLALEVARGSGHYSVFARSAVAYNSPRVGGDERALNVLGQAEVGALYEFAPR
                     AVNSAYVGLSAGALLLRFAGRLEPSDPKSADAFTTAGAMLSARVGVRFLRIYDFDFDA
                     FGAVVAPLFASKNPDSVLFGTSGAYTPVAELGLGVGF"
     gene            complement(1718..2977)
                     /locus_tag="VFQ35_08495"
     CDS             complement(1718..2977)
                     /locus_tag="VFQ35_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930711.1"
                     /translation="MRIHGLSLGLLPVAMLTSPVLAKPRETSVCVDVVVERAAEKPAA
                     SGERSPEKAAPTAPPVASASPATSSEPPEVPPPPAANAELTPPLGPRVDAALAAQAAA
                     SVDPEGSRDPRVQSGANLPLGQTPEAYLGRLIEHFVTHERGFLAVKSGCEQRIVVDLY
                     PLRLGWTVFASYTGTGREERVDQLLPTELSQFAERSVLALLRDVPISETVDRNNVLWA
                     DSLKSTQSIRGRGHFVIGVGTRLRGGAFHTANDAGSARATELRPFAPVLLTAGYRGQF
                     ESFGVEAAGEVDIGTSQVAAQRNPSGGHVDYGGSMGFSLHAMRYLNARGLSSLYIGGG
                     ATFALHWFPIIKPEMDRDSGPRNTLWSGGLDVDGVIGYELMRASAISFYLQGEINLPA
                     YVVRNENAAGSVDTWFPGAALKLGTIF"
     gene            3461..4237
                     /locus_tag="VFQ35_08500"
     CDS             3461..4237
                     /locus_tag="VFQ35_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9930712.1"
                     /translation="MQAIDGDARDGAIDLFLRSMPSPYAHVFERSDVVAHARIVAERG
                     DKVAHVALWRSLPGGGAALCVVADDRPGLLAHVTAAFGAHQLDVMSAQIFCRTTGGGT
                     DEAVDFFWLRPLRNGRVIGDEDIALVANTLRELLLDDELERDSLKRPSTIPAGPASGT
                     RVYFDVRALQRGESVLMVETKDTPGLLFAITSELYRQGVEVVASDVRTHGQVAFDRFT
                     LTDAEGRALSGAKLAEVQQGVRRAVRALVPGSLKRNPNLI"
     gene            complement(4250..5113)
                     /locus_tag="VFQ35_08505"
     CDS             complement(4250..5113)
                     /locus_tag="VFQ35_08505"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9930713.1"
                     /translation="MTDLISATLCDDLLAVFDDPNYRPPPLPEVALELMSIGGREETN
                     VAEVVGLLERDEMLAATVLRLVGSPIYAGRTGIRSLKEAVVRLGVRGVRDAVFEAALR
                     RGVFVMGEYGETMASIARHSTVAAYITRIVCRRAAIDQEIAFICGLLHDVGFAGLLLA
                     ITQIEGTAAPPLVKLWKDVDALHEHASLLLATRWSLPAPLVEVVGHHHHLHTGPCATL
                     AAAVRLADHLTQHFGATVRGPVVNGEPLGGDCVELFDVESACAELNIDDTALKLIIAD
                     AERTVPEIVLL"
     gene            5264..5818
                     /locus_tag="VFQ35_08510"
     CDS             5264..5818
                     /locus_tag="VFQ35_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930714.1"
                     /translation="MLPPPPPKGVRDVLDDPLLPFVVAAKAGDKGAERELLVRVAPTV
                     VSVARAAGAGVEPTLSTVVLDGLISTLKALPTFRGEELVRETVARITLEKAKLSLGTL
                     AADDDVILRTARSPQVRGRSGESSHIGDWVDRALQDDAAVLVSHVLLRTAHKRSKRRP
                     YTWRGIAVIGLATVIGFWLLARLL"
     gene            complement(5954..6646)
                     /locus_tag="VFQ35_08515"
     CDS             complement(5954..6646)
                     /locus_tag="VFQ35_08515"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HET9930715.1"
                     /translation="MAPLERLALLSIVFFAIHALVSGTRLRTTLVRALGERAFRGLFA
                     LASLVSLGALWLAYRSAPCAPLWVLPRAFGWFPLLVVPFALFFVAGAFSTPNPTAIGG
                     ERALERGEPARGILRITRHPFLWGVILWSSAHLLVLGGAASLWFFGSLWLTAALGTRS
                     IDRKRAASQGDAWQSYRSVTSNLPFAAIFGGRNRLQLRELRLPAVIAAVLTAALLASH
                     RWLFDVSPFPDY"
     gene            complement(6654..7136)
                     /locus_tag="VFQ35_08520"
     CDS             complement(6654..7136)
                     /locus_tag="VFQ35_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET9930716.1"
                     /translation="MNIYANSTRTLSGEATSLSRFSGQVTLIVNVASECGYTPQYAGL
                     ERLQQRFKGKGFSVLGFPSNDFGGQEPGTPEQIQTFCSTRYGASFPMFEKVSTKPGPE
                     QSPVYAALGEAAGALPKWNFSKYLVGRNGQVIAFYPSSVEPEDPKVLAAIENALAAQA
                     "
     gene            complement(7193..>8093)
                     /locus_tag="VFQ35_08525"
     CDS             complement(7193..>8093)
                     /locus_tag="VFQ35_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET9930717.1"
                     /translation="ILEEIDKKITKQEIIDAANMAKKYGVHVRFYMMLGNRGETAETF
                     RETLDFLSVAKPHQYIFSCLSIYPGTKDFHDAEAAGWLEREEYFRGDFQELKVPFDAS
                     EADTRVMTEWFAENHGLRDYYLEGVDECRAILDRLGDHAPAHLDLAGAYYRVGDFERT
                     EHHAVRALELGFPAPGLAHNYLAVIAYRRGDVQRMQDEFMKAAKLDPQHYVLMRNVDR
                     ARAWFRERGPERRLPLDLVARHDFQLFERTQQPTLPGPLPDDVLRWDAEDRASPQSAA
                     DDVLRIDTGNESIPFRPKRLPVV"
BASE COUNT         1428 a         2701 c         2630 g         1334 t
ORIGIN      
        1 cggcaacgaa gaccactact cttggtcgtt cggcgtcggc gtcgagcgcg tggctcgtgc
       61 ctcgctgatg ctccgatatc ggctgttgcc gtacctgtac gcggcgttcc tggaagcgag
      121 cgaaacaggc gctcccgtgc agcgtccgct ggtgttcgac ttccaggacg acatcgagtc
      181 acgccgcatc gacgaccagt tcttgctcgg gagctcgctt ttggtcgccc cagtactgaa
      241 ggctggtcaa accgcgcgcc acgtgtacct gccgcccggc gattgggtcg actttcacag
      301 cggagaacgc cacgccggcg aatcgtacgt cacgctcccg gccccgctcg gaaagatccc
      361 gttgctcgtt cgcgccggcg ccgtcattcc gatgctcgag cgcgctcccg acagcacaca
      421 cggtctcgcg ccagagctca tcgagctcca cgtcttcgct ccagggcgcg acggctcgta
      481 cgagagcctc ttgtacgaag acgacggcgt ttctcgcgca cacgagcgcg gggctcacct
      541 ccggacgcgg ctgacgttgg tacgcagcgg tgcgacgctg ctactgcgcg ccgcggtgag
      601 cgggcagagc ttcccccaat ttcagcgtcg tcgcctacgc gtcgtgctgc acgccgagac
      661 cccgctcgaa gcgacgctcg acacacgacc gctgagcgtc gacgcctcca acgtcgtttt
      721 cgacgatcgc ggcggcggct tcgagctgcg aatcacgctc gcttgattca gaacccaacg
      781 ccgagcccga gctcggccac gggggtgtac gcgccgctcg tcccgaagag gacgctgtcg
      841 ggattcttgc ttgcgaagag cggcgcgacg acggcgccga atgcgtcgaa gtcgaagtcg
      901 tagatgcgta ggaaccgcac cccgacgcgc gccgagagca tcgcgcccgc ggtggtgaaa
      961 gcgtctgcgg atttcggatc gctcggctcg agtctacccg cgaaacgaag gagcagtgca
     1021 cccgcgctga ggccgacgta ggcgctgttc acggcgcgcg gcgcgaattc gtagagagcc
     1081 cccacctcgg cttgcccgag cacgttgagc gcgcgttcgt caccgcccac ccgcggcgag
     1141 ttgtaagcga ccgcgctccg cgcgaaaacc gagtagtggc cggagccgcg cgcgacctcc
     1201 agcgcgaggc cgggcgcgaa agcgagcccg ttgtcggtgc gagccattcc ctcgaagaag
     1261 gccaagcgat agctgttgct gccgtgcacc agcaccgcgc gcgtggcacg ttcgcccgcc
     1321 gaaagccgcg aaggatcttc ggccaagtac gctggatcgc tgttcaggac gcgagatatc
     1381 ccttccgaaa ggcggatctc gaggtcgcga tcgctttgca gagtgtagcg aagcggcacc
     1441 tcttgatcga tgcgcagcgt caggtagcgc gacttgccga gccagaagag ttccgcttcg
     1501 aggcgactgt cgcaagccgt ctcgcccacg cgatggctcg ggtgacgggc gagctccgag
     1561 cgcacgagct tctcgaaacc cgctcgttcg gcgtcgctcg ccttgatgtc gacacacaac
     1621 gcgacgctcg catacgcccg caccggtgcg atgcaaacca tgaccgacgc gccgatgatg
     1681 cgcgcggcgc gttcgagcca acgcttcacg ctcgacctca aaagatggta ccgagcttga
     1741 gcgccgcgcc tgggaaccag gtgtcgacgc tgccggcagc gttttcgtta cggaccacgt
     1801 aagcgggcaa gttaatttcg ccctggaggt agaacgaaat cgcgctcgcg cgcatgagtt
     1861 cgtatccgat caccccgtcc acgtcgagcc cgccgctcca caacgtgttg cgcggccccg
     1921 agtctcggtc catctccggc ttgatgatcg ggaaccaatg caacgcgaac gtcgcgccac
     1981 caccgatgta gagagaggac agtccgcgcg cgttgaggta gcgcatcgcg tgcaggctga
     2041 accccatgct gcccccgtag tccacgtgtc cgccgctcgg gttacgctga gcagccactt
     2101 ggctcgtccc gatgtcgacc tcgcccgccg cctccacccc gaacgactcg aattggccgc
     2161 ggtaacccgc ggtgaggagc actggcgcaa acggccgaag ctcggtcgcg cgcgcgctgc
     2221 ccgcgtcgtt ggcggtgtga aaagcgccgc cgcgcagccg ggtaccgaca ccaatcacga
     2281 agtgaccacg gccgcggatg ctctgcgtgg acttcaacga atcggcccag agcacgttgt
     2341 tgcggtccac ggtttcgctg atggggacat cgcgcaacag cgcgagcaca ctgcgctcgg
     2401 cgaactgtga gagctcggtc ggcaagagct ggtccacgcg ctcctcgcgc ccagtgccgg
     2461 tgtagctcgc gaacaccgtc caaccgagcc gcagcgggta caagtcgacg acgatgcgtt
     2521 gctcgcagcc actcttcacc gccaagaagc cgcgctcgtg cgtgacgaag tgttcgatca
     2581 gcctgcccag atacgcctcg ggcgtctgcc cgagcggaag gttggccccg ctttgcacgc
     2641 gaggatcgcg gctgccttct gggtcgacgc tcgcggccgc ttgcgcggcg agcgcagcgt
     2701 ccacccgcgg gcccaaaggc ggcgtgagtt cggcattggc agccggcggc ggaggcacct
     2761 cgggcggttc actcgacgtc gcgggcgatg cgctggcgac gggcggcgcg gtcggcgcgg
     2821 ctttctccgg cgaccgctcg ccgcttgcgg cgggcttttc ggccgctcgc tccaccacga
     2881 cgtccacgca cacgctagtt tcgcgaggtt tagcgaggac gggcgaggtg agcatcgcga
     2941 ccggcaacaa tcccaagctc agaccatgaa tacgcatata actcccacgg gtgattacga
     3001 caccggcgaa ggcgaactac cccacccccg cgatttgctc cccgcacagg gcgcctccgc
     3061 cggcactcga cgcttcagca atggacgtgc caccgaaact cgcgcgcctt cacctgacga
     3121 aaccagcgtt atacgcgtgg cgagagcgac ttcttccctg ccatgcaccc gcacttcgag
     3181 cgtgaccttc gggcgacggg cgtggctagg agtcacagtc gcgcagcggc gttccttcac
     3241 acggagctct ccggggcgcg acgaacttca ctcgtggaga ctcggtgcct gggtgtcagc
     3301 gctcgcgaga agcgcgaggg tcgcgcgctt gctctcatga gtgttgcttt tggcgttgtt
     3361 gtgaggcaat ctcgcacagg cgctttcgcg cgtgcgctgc gcaaacggaa acgactccga
     3421 aaagcagccg caacgcgcgc tctgcgatcc tcacgtcctc atgcaagcga tcgatgggga
     3481 cgcgcgcgac ggtgctatcg acctcttctt gcgctcgatg ccgagcccgt atgctcacgt
     3541 cttcgagcgg agcgacgtcg tcgcccacgc gcggatcgtc gccgagcgag gcgataaagt
     3601 cgcccacgtc gcgctgtggc gatcgctgcc gggaggaggc gccgcgctct gcgtggtggc
     3661 tgacgaccga ccggggttac tcgcgcacgt gacggcggcg ttcggggcgc accagctcga
     3721 cgtgatgagc gcgcagattt tttgccgaac gacggggggc ggaacggatg aggcagtcga
     3781 ctttttttgg ctgcggccgt tacgcaacgg ccgcgtcatc ggggacgaag acatcgcgct
     3841 cgtcgccaac acgctgcgcg agctcctgct cgacgatgag ctcgagcggg attcactgaa
     3901 gcgcccttcg accatccctg ccggcccagc ctcgggaacg cgagtctact tcgacgtgcg
     3961 agcgctgcag cgtggcgagt cggtgttgat ggtcgaaacg aaggatacgc ccggcctcct
     4021 tttcgcaatc acgagcgagc tctatcgaca gggcgtggaa gtcgtcgctt cggacgtgcg
     4081 cactcacggc caggtcgcat tcgatcgctt cacgttgact gacgcagaag ggcgcgcgct
     4141 cagcggcgcg aagctcgccg aggttcagca gggtgtacgg cgtgccgttc gtgcgctcgt
     4201 cccgggttcg ctgaagcgca accccaacct catttgagtg cggctcgcgc tagaggagca
     4261 cgatctcggg aacggtgcgc tcggcgtcgg cgatgatgag cttcaatgcg gtgtcgtcga
     4321 tgttcagctc cgcgcacgcg ctctccacgt cgaagagctc gacgcagtcg ccgccgaggg
     4381 gctcgccgtt cacgacgggt ccgcgcaccg tcgctccaaa gtgctgcgtg aggtggtcgg
     4441 ccaagcgcac ggccgcggcc aaagtcgcgc acggccccgt gtgcaagtga tggtggtgac
     4501 cgacgacttc gaccagcggc gccggcaagc tccagcgcgt cgcgagaagc aacgaagcgt
     4561 gctcgtgcag agcgtcaaca tccttccaaa gcttgacgag cggaggcgcc gcggtgccct
     4621 cgatttgcgt gatggcgagc aggagccccg cgaagcccac gtcgtgcaac agcccgcaga
     4681 tgaaagcgat ctcttggtcg atcgcggcac gccggcacac gattcgcgtg atgtaggcgg
     4741 cgaccgtact gtgacgagcg atgctcgcca tcgtttcacc gtactcgccc atcacgaaga
     4801 cgccccgccg gagcgcggcc tcgaagaccg cgtcacgtac accgcgcacg ccgagccgaa
     4861 cgaccgcctc cttcaacgag cggatcccgg tgcgccccgc gtaaatcggc gagccgacga
     4921 gacgcaatac cgtcgctgcc agcatctcgt cgcgctcgag cagcccaacc acttcggcga
     4981 cgttggtctc ttcccgtccc ccaatcgaca tcagctcgag ggcgacctcg ggaagcggcg
     5041 gcgggcggta gttcggatcg tcgaataccg cgaggaggtc gtcgcagagc gtggcggata
     5101 tcagatcggt catcgaaaga acgcggtcgc tcccgctgat taacggccga ttttcccgca
     5161 gtgaaaggcc gaggcaagta gcctgcgtgg aaatcccccg cccggcgctc gaccgcggcc
     5221 cgattctcgc ctaaattcgc agttctcgcg aggcgaaata ctcgtgctgc cccctcctcc
     5281 gcccaaaggc gtgcgggacg tactcgacga cccgttgttg ccgttcgtgg tcgccgcgaa
     5341 agccggcgac aagggtgcgg agcgcgagct gctcgtccgt gttgcgccta cggtcgtctc
     5401 ggtcgcgcgc gctgccggcg caggcgtcga accgacgctt tcgacggtcg tgctcgacgg
     5461 cctcatttcg acgctgaagg cgctgcctac atttcggggt gaagaactcg tacgagagac
     5521 cgtcgctcgc atcaccctcg agaaggcgaa gctctcgctc ggtacgctgg cggccgacga
     5581 cgacgtgatt ttacggacgg caagatcacc ccaagtgcga gggcgctcag gtgaaagctc
     5641 gcacatcggc gactgggtcg accgagcact ccaagacgac gccgccgtcc tcgtgagcca
     5701 cgtcctgctc aggaccgctc acaagcgaag taagaggcgt ccctacacgt ggcgcggcat
     5761 cgccgtgatc ggactcgcga ccgtcatcgg cttttggttg ctcgcgcgtc tcttgtgatg
     5821 cgctccgcga aggccgagaa cgtcgcctcg attcacccga ccggaccagc tcgtggcggg
     5881 cgaagaacaa cgagccgagc gactgctcgt ggcgggcgaa gaacaacgag ccgagcgact
     5941 gctcgttgcg agctcaatag tcgggaaacg gcgacacgtc gaagagccag cggtggctcg
     6001 ccagcagcgc ggcggtgagc acggcagcga tgacggcggg caagcggagc tcgcggagct
     6061 gaaggcgatt gcgcccgccg aaaatcgcgg caaacggcag gttcgacgtc acgctgcgat
     6121 agctttgcca agcgtcgccc tggctcgcgg cccgctttcg gtcgatgctc cgcgtgccga
     6181 gggcggcggt gagccagagg ctcccgaaaa accagagcga cgccgctccg ccgagcacca
     6241 acaggtgcgc gctcgaccac aagatgaccc cccaaaggaa cgggtgccgg gtgatgcgca
     6301 gaataccgcg cgccggctcc ccgcgctcga gcgcgcgctc accaccgatc gccgtcgggt
     6361 tcggcgtact gaacgcgccg gcgacgaaga acaaagcgaa cggcaccacc aagagcggga
     6421 accacccgaa cgcgcgcggc aatacccaga gcggtgcgca cggcgccgaa cgatacgcga
     6481 gccacaacgc gccgagcgac acgagcgagg ccagcgcgaa caagccgcga aacgcgcgtt
     6541 ctccaagcgc cctcaccagc gtcgttcgca agcgcgtacc ggacaccaag gcgtggattg
     6601 cgaagaaaac gatcgaaagc agcgcgagcc gctcgagagg cgccatgttt ctctcaggct
     6661 tgcgcggcga gcgcgttctc gatcgcggcc agcaccttcg gatcttccgg ctctactgac
     6721 gacgggtaga acgcgatcac ctgcccgttg cgcccgacga gatacttgct gaagttccat
     6781 ttcggcaatg cgcccgcggc ctcgccgagc gcggcataga ccggcgactg ctccggcccg
     6841 ggcttggtgc tcaccttctc gaacatgggg aagctcgcgc cgtagcgcgt gctgcagaac
     6901 gtctggattt gctccggcgt gccgggctcc tgaccaccga aatcgttgct cggaaacccg
     6961 agcaccgaga agcccttccc cttgaagcgc tgctgcaggc gctcgaggcc ggcgtactgc
     7021 ggcgtgtagc cgcactcgct cgcgacgttg acgatcagcg tcacctgtcc ggaaaaacgc
     7081 gagagcgacg tcgcctcacc tgacaatgtt cgcgtgctgt tggcgtagat gttcatggtg
     7141 cctcgtgagc gaaaacttgt cgggcgagca gcgctcgtca agcctcgact cgtcaaacta
     7201 ctggcaagcg cttggggcga aacgggattg attcgttgcc ggtatcgatg cgtagcacgt
     7261 cgtcggcggc gctctggggg gaggcccggt cctcggcatc ccagcgaagc acgtcgtcgg
     7321 gcaagggacc gggaagtgtc ggctgctgcg tccgctcgaa cagctgaaag tcgtgccgcg
     7381 cgacgagatc cagtggcaga cgccgctccg gtccgcgctc tcgaaaccag gcgcgagccc
     7441 ggtcgacgtt tcgcatcaac acgtagtgtt ggggatcgag cttggccgcc ttcatgaatt
     7501 cgtcttgcat gcgctgcacg tcgccacggc ggtaggcgat cacggccaag tagttgtgcg
     7561 cgagcccggg cgccggaaat ccgagctcga gcgcgcgcac cgcgtggtgt tcggtccgct
     7621 cgaagtcccc tacccggtag taagcaccgg cgaggtccaa atgggccggc gcgtggtcac
     7681 cgaggcggtc gaggatggcg cggcactcgt cgaccccctc caggtagtag tcgcgcaagc
     7741 catggttttc ggcgaaccac tccgtcatca cgcgtgtgtc ggcctcgctc gcgtcgaagg
     7801 gtaccttcag ctcttgaaag tcgccgcgaa aatactcctc ccgctcgagc cagccagccg
     7861 cttcggcgtc gtggaagtct ttcgtaccgg ggtagatcga caagcacgag aaaatgtact
     7921 ggtggggctt cgcgacgctc aaaaagtcca aggtttcgcg gaacgtctcc gcggtctctc
     7981 cgcggttgcc gagcatcatg tagaagcgca cgtgcacgcc gtacttcttc gccatgttcg
     8041 ccgcgtcgat gatctcctgt ttggtgatct tcttgtcgat ctcctcgaga atg
//
LOCUS       DASTKS010000408         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1403852, whole genome shotgun sequence.
ACCESSION   DASTKS010000408 DASTKS010000000
VERSION     DASTKS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403852"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFQ35_08530"
     CDS             <1..769
                     /locus_tag="VFQ35_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930718.1"
                     /translation="PNPLPNANSNDFASPDPFPSTNPHEFTNVDSSARGDANESPGRE
                     LPAPPLPTRALQRTNPNQIRLIWPATLPIMPVPDLDALRNAAQPEPADALVESPWLDA
                     VHVPRLRGSLDTSVRLTWLDRMLTSPRFKPAIITAASVFLLMGISVLFAFALDSDSDD
                     YAPAESELPAKVLPKDSPKAGQAPSKPVNPDKVATPPSTTAAPEHASTSPKSAAGAAT
                     PAPPHAPGSPHMATRSTPREGARPRTNNQGARVPWWE"
     gene            760..1812
                     /locus_tag="VFQ35_08535"
     CDS             760..1812
                     /locus_tag="VFQ35_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9930719.1"
                     /translation="MGVRRTLAALGLMSGVCMVTHSARAEPNPSDDPRQQARDLMRAG
                     NDRYRSGDLKGARESYQRAFDTAKSFDVACNLGRTEMELQLFRDAAEHLDYCVRFHIL
                     SSKPEMMLNEQEARDKLRAAKARVGSLWLSVEPSAAEIWVDSRMVGRAPLVAPVFVEP
                     GPHEVRVTSPQMAESTTRVAARAGTEQTLKIQLSPESSGANFAGAMPGSTFPASDELQ
                     RKHSSWGVVPWLSGGLSLVGIGVGAGFLVVAHDKQSDKEDVLTALPEAHPCAENTPHV
                     NECARVRQLAQDGRTAQAIGYTSLGVGVAAAVTTAVLLLQSPHTTTASKRHAPQARVV
                     PSVSSDRLFVSLDARF"
     gene            1830..>2919
                     /locus_tag="VFQ35_08540"
     CDS             1830..>2919
                     /locus_tag="VFQ35_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930720.1"
                     /translation="MKTYLPFAALTLAPLVAFAACDSGPCENTGSCGDYPGGAAGTSG
                     TTSTSSGGGNGTGGRGGTSSGGVTSEAGAAGGPANGGAAGGGNEAGAGGESGGAGGAG
                     GAPSTCDVTQSPSTEPCLVSDEFAMFVAPTGDDSSSGTQDAPVKSLSRALELATLSGK
                     LVIVCNARFDEQLVLTSGARVYGGFACPTEDTPWLYAPGTRTEVKPVTEGAALIVQGV
                     LDPVVIEDLDFASMTATRAGSSSIGASIVNSPDVTLRRTRITAGSAAAGANGAKGANG
                     PDGDLPLSDQRGGDAVCGAQAPATRDGGRWLAANACGSRGGAGGQAQQGDLGFPGASG
                     TPQINVVPEAVNNGGSAAPATPANAPGGA"
BASE COUNT          510 a          989 c          988 g          432 t
ORIGIN      
        1 cccgaacccg ctgccgaacg ccaattcgaa cgacttcgcg agccccgatc ccttcccgag
       61 cacgaacccg cacgagttca ccaacgtcga ctcatctgcg cggggcgacg cgaacgaaag
      121 ccctggtcgc gagctccccg ctccgccact gccaacgcgc gccctgcaac gcacgaaccc
      181 gaaccaaatt cgtctgattt ggccggcgac gctgccaatc atgccggtgc cggacctgga
      241 cgcgctgcga aacgcagccc aaccggaacc ggcagacgct ctggtcgaat caccgtggct
      301 cgacgcggta cacgttccgc ggctgagagg ctcgctggac acgagcgtgc gcctcacgtg
      361 gctcgatcgc atgctcacgt cccctcgctt taagccggcg atcatcaccg ccgcgagcgt
      421 gttcctcctc atggggatct cggtcctgtt tgcgttcgcg ttggactccg attccgacga
      481 ttacgcgcca gccgagtcgg agttgcccgc gaaggtgctg ccgaaagact cgccgaaggc
      541 agggcaggcg cctagcaagc ctgtgaatcc ggacaaggtc gcgacacctc cgtcgacgac
      601 cgccgcgccc gaacacgcga gcacctcccc caagtcggcc gcaggcgcgg caacgccagc
      661 cccgccgcac gcgccgggaa gcccgcacat ggccacgcgc agcacacccc gcgaaggggc
      721 gcgtccgcga acgaacaacc aaggagcgag ggttccgtgg tgggagtgag acgaacgctc
      781 gcggcgctcg gcttgatgag tggcgtatgc atggtcactc acagcgcgcg tgcggagcca
      841 aacccgagcg acgatccaag gcagcaagcg cgcgacctca tgcgtgcggg caacgatcgc
      901 taccggagcg gagacttgaa aggcgcgcgt gaatcgtacc agcgagcctt cgacacggcg
      961 aagagcttcg atgtggcgtg caacttgggc cgcacggaga tggagcttca gttgttccgt
     1021 gacgccgccg agcacctcga ctattgcgtg cgtttccaca tcttgagcag caagcccgag
     1081 atgatgctca acgagcaaga ggcgcgcgat aagctgcgcg cggcgaaggc acgtgtcgga
     1141 agcttgtggc tcagcgtgga accgagtgcc gcagagatct gggtggattc gcgcatggtc
     1201 ggcagggcac cgctcgtcgc gcccgttttc gtcgagccgg ggcctcacga ggttcgggtc
     1261 acgagcccgc aaatggccga gtctacgacg cgcgttgcgg cgagggccgg aaccgagcag
     1321 accttgaaaa tccagctctc gccggagtcg agcggggcga acttcgcggg cgccatgcct
     1381 ggctccacct tccctgcgag cgacgagcta cagcgcaagc actcgtcgtg gggcgtcgtg
     1441 ccgtggctct cgggcgggct ctcgttggtc ggcatcggtg tgggcgccgg cttcctcgtc
     1501 gtcgcgcacg acaagcaaag cgacaaagag gacgtgctca ccgcgctgcc tgaggcacat
     1561 ccgtgcgccg agaatacgcc tcacgtgaac gagtgcgccc gcgtgcgaca gctcgcacaa
     1621 gacgggcgca ccgcccaagc aatcggttac acgagcctcg gcgtcggcgt cgccgcggcg
     1681 gttacgaccg cggtgctcct cttgcagtcg cctcacacga cgaccgcgag taagcgtcac
     1741 gcgccacaag ctcgcgtcgt cccctcggtg tcttcggatc ggctcttcgt gagcctcgac
     1801 gcgcgcttct gaagcttgga ctcgagacta tgaaaaccta tcttcctttt gcagctttga
     1861 cgttggctcc gctcgtcgct ttcgccgcgt gcgactcggg gccttgcgag aatacgggtt
     1921 cctgcggcga ctacccgggc ggtgcggcgg gcacttctgg cacgacgtcg acgtcgagcg
     1981 gaggaggcaa cggcacgggc ggtcgtggcg gaacgtcgag cggaggcgtt acgagcgaag
     2041 ccggcgcggc aggcggaccg gcgaacggag gcgcggccgg cggaggcaac gaggcgggcg
     2101 ctggcggtga gtccggcgga gcgggcggag cgggtggagc gccgtcgacg tgcgacgtga
     2161 ctcagagccc gagcacggag ccttgtctcg tgagcgacga gtttgccatg ttcgtagcgc
     2221 cgaccggcga tgattcgagc agcggcacgc aagacgcgcc ggtgaaatcg ttgtcgcgag
     2281 cgctcgagct cgcgacgctc tccggcaagc tcgtcatcgt gtgcaatgcc cgcttcgacg
     2341 agcagctcgt gctcacgagc ggggcgcgag tctacggtgg tttcgcgtgt ccgaccgaag
     2401 acacgccctg gctctacgcg ccgggaactc gcaccgaggt gaagcccgtc accgaaggcg
     2461 ctgcgctcat cgtccaaggc gtgctcgatc ccgtcgtcat cgaagatctc gatttcgcct
     2521 ccatgaccgc gacgcgcgca ggcagctcga gcatcggcgc gtccatcgtc aactctcccg
     2581 atgtgacgct gcgccggaca cgcattaccg ccgggtccgc cgccgcaggc gcaaacggcg
     2641 cgaagggagc caacggcccc gacggcgact tgccactcag cgatcagcgc ggcggagacg
     2701 cagtgtgcgg agcgcaagcg cccgcgacgc gcgacggcgg gcgctggctt gcggcgaacg
     2761 cgtgcggttc gcgcggcggc gctggcggcc aagcccagca aggcgatctg ggctttcctg
     2821 gcgcttcggg cactccgcaa atcaacgtcg tccccgaggc ggtgaacaat ggcggtagcg
     2881 cagcgcccgc aaccccagcc aatgcgcccg gcggcgccg
//
LOCUS       DASTKS010000409        10203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_287175, whole genome shotgun sequence.
ACCESSION   DASTKS010000409 DASTKS010000000
VERSION     DASTKS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287175"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..1681
                     /locus_tag="VFQ35_08545"
     CDS             467..1681
                     /locus_tag="VFQ35_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HET9930721.1"
                     /translation="MWLLDAAHCDACLSNDEVVGLARSQGTPLFVIDCDQVRRQYVLL
                     RTALPGVNLHYAIKALPHPEVVATLRDEGSSFDVASSGEIELLRSQGVAPERALHTHP
                     IKSELEISRSLAWGCNTFVVDNAAELQKFAPFGAVVKLVLRVSFRNPNAAFDLSKKFG
                     CAAHEALDLLCFARRLGLRVRGLSFHVGSQCSSSGAHVQAIGACRDLILAASAAGFPP
                     LDTLDIGGGFPISYSPEPIDIDAFCAPIRKALATLPRGMRLLAEPGRFIVGPAAHSVA
                     SVVGRSEREGSLWYYLDDGVYGSYSGRLFDHAGYPISVLASVARPLRASCLAGPTCDS
                     VDVIAENILLPELELGDLVVGHTMGAYTSATASEFNSFEKAKVFVLNAPSAVVGEGAT
                     THTRMLAVRKAE"
     gene            1803..3398
                     /locus_tag="VFQ35_08550"
     CDS             1803..3398
                     /locus_tag="VFQ35_08550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930722.1"
                     /translation="MLAKNAKPAVAKMSRTSHKDVPAPGAGNTPNASSFEPGMVVGGK
                     YRIGSLIGVGGVGRVYLAKHIELDTDVAIKVLRPEMLQRPDVVQRFGREARASVRLRS
                     ERIARVFDVGVEHGVPYFVMECMVGCDLLDFRLQNNPDIATVAELFIQACEGLAEAHA
                     QAIVHRDVKPENLFVVRDENGWRTLKILDFGISKFSLTGKHSDVDLSAKRTEAMLGTP
                     NYISPEQIRATRDVDLRTDLWSLGVVMFEVLSGGVLPFREEREMTALIAEILEMPHRS
                     LLDVAPNVPEGLVAIVDRCLEKDRDKRYQTAAEIALDLLPFAPVRARASVERAVSAMQ
                     AAGLLPVNLDLSSMRSSQPPIERLSVSGMQNRLRSTLPESLDAVEISEANFRPSKRWV
                     APLALTLVVAGLTYGATTLLREQASEPVKVSSGEPVKVSSGERIGEARLPEPLLPPPS
                     SSPEPVASAHAPQSAPRAPASAAVATARPSVSPRPPVAFAPRDFRPHPAVNSALPAPK
                     PVVSTKAEAASNGAPKLDLRRER"
     gene            3428..6448
                     /locus_tag="VFQ35_08555"
     CDS             3428..6448
                     /locus_tag="VFQ35_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9930723.1"
                     /translation="MWKRQAARTRLGLMILVCGLGGGIVPSRANASETADEADVEFQL
                     GADRYDAGDYKQALSHFLASNRLAPNKNVLFDIARCYEQLKLLPEAFRYYSLSLEAET
                     SEAGKHRIQESLEALKPRVARLTIESDPPGAVIYLDRKDLGSRGLTPRTLALPEGEHT
                     VILELPGFEPGRSRSVRVKLGEETKANIELTPILGSVEVVGTQGAAVRLDSEANPVLC
                     SVPCSFRAPPGKHVVLLSKPGFGSREVPVEVGVNHAERVRAKLEPLSGSVVVNADVRE
                     ALVSIDGQPRAFTPAVVSVPVGEHEIVVSQSGFRPVRRRVRVETEQSVRLDFELSGQE
                     EVLGASRKAEAAEDAPASVTIISREELRAMGYPTIAEAIRGVRGMYLSYDDTYYSPGV
                     RGFARPGDYGNRILVLVDGHATNDNWIWTSYIGFDARVDIDDVERIEVIRGAGSVVYG
                     TGAFFGVINLVTRSNDQPTHGEVAVSTAQGAGRARATAVWHPRPDAGAWLSVAGAQAA
                     GLDRYYPEYVSEPTDAGPSIVDYRGNPANGVVRDSDGFRAGTLGGHAFYKALSTRWLL
                     TAHKKHSPSAQYETLFGDPRTSNSDTRGFVDVEFEPTSEHLESLTRVHFDYYRYANRL
                     PYAPNSVDPASFGLENDRYTGVWGGVEQRFTIKPTQALKLTVGGDFTQHFTTHQRNVD
                     DRGRAGYTGPDAGPIIDNNNPFKNVAGYALLDAAISSQLRVSAGARIDYFDKLDFDAV
                     AALSPRVAIIIKPYARGNLKVMAGKAFRVPSVYERFYVSTTQIAPTRIKPEQVFSGEI
                     EFSHRFSNAVTGLVTGYTNYVTNLIQLGTVTRGDATYNQYGNSDAPVLVFGGEAEVRR
                     EWRQGFMLTASTSVQKAEYLSDDNLRRVPNSPLVLGSAKVVVPVIGRALVLASKLSIE
                     GARYDNQYRNEDVTCDPEGAVVTPCPRQRTTDTGIVWDLVLSGAVERYNANYTLGVYN
                     VMDWKYDTVPSTEYLQRTIRQRPRSLLASLAFKF"
     gene            complement(6492..>10203)
                     /locus_tag="VFQ35_08560"
     CDS             complement(6492..>10203)
                     /locus_tag="VFQ35_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930724.1"
                     /translation="ELLASLDHPGIVKTVAFGVLEDTGLPFVAMEWLDGEDLAARQRK
                     NPLSIPEALELTLRVAEALSAAHQAGVIHRDIKPGNIFLCRQLEKPPGALLDVWPKLV
                     DFGVASHGEMRVRRAGDLVGTPAYMAPEQARGDAPLDGRSDIYSLGATLFELIAGRPP
                     HVGPNVIATLARLATTPSPHLSELRRDVPPVVDSLVQSMLETDPAERPRSAQELCEIL
                     RSATEDSSRTSWFDSEPRSTRLGSSGSRLITSIVALRFESDSARERALESMRQRGADA
                     VPLGRDSIVAHLGARRAVGSEALVALELARRLARAGARVGLASGRAWVNLLSDSVVHP
                     VGEVVDRASALARDAEPGTVLADVTTSELGRGRYEFRARDDGSAIVGEPLRGGRGDRA
                     GGAPFVGRDAELAQVLSAFSRARSERTPILVTITGPPGIGKSRLRREVMARIAAQAEA
                     PQIILQRSEAYGQGQSLGAAADVLRAIIGLPKGATSSEAARAIVARLGPATRDELTAR
                     TRDLVARLLANEPLSEGLDPRGSRDALWLAMTDLVMQITAVESTAILVEDLQWADPES
                     IGWLDHLMGRAANRPLLVMAMVRPEFWTANPERFAGRDHVRLELRPISRRSARSIVQS
                     VLGPSTPEAVLDRIAEQAGGLPLFAEELARLTAAGRDTLQAPTIQAAIQVSLDALDDE
                     CRDAVGRLSVLGLTCWESALEAFGMPGAEGVMKALVAHEVLVEQNNSRFSGTREWTFK
                     HALVRDVAYASLGERERKELHALAGTWLASMGEDAAVVAGHFDLGAQHAAAAEHWTRA
                     AQRALSTNALTDALNMAERALAFAEDRRSGFVRASLLDEAWSRLDPRASERESAIMAL
                     EANVFDEASAVRARGARARFDEARGSGTGISERLAETRDEAAALGLHDEEARCSAVLA
                     SRLAFAGRFAEAEAEVRRLLQLAEQRGISAAAVDAYQTQAIIRQTQGAVGAALESRRN
                     AVAAAHKAALREREAMLMTNLGFALTTIGARQEARASLDSGLALADAIGSQGAMRNAQ
                     MNLLGWCATFGSDKMLDAHLAQVRTDADATASGIWAAPDRANLGILFYRGCELLRQKS
                     ERDYRRALGLLRMAAQGYKTTGHRDLLPVALGMWAEAERLSGNVSAAAELAREAIAML
                     ERGSPSLLNESPVYLALHDALVDLGDETGARDAIRRGLPALARRLQALVGTPYARLFL
                     TDLQQNARLVAIAEEDGLVPDTIHRVMEGGRS"
BASE COUNT         1660 a         3402 c         3416 g         1725 t
ORIGIN      
        1 tttggtagta ctcggcgagg taatccgccg gcacccaggt gctgaagtcc ttttcggtca
       61 tgaggatctg cgtcggcgcg ccggccgata tttcgccgcc aaatcgtgtg gaatcggtcc
      121 caggttctac cacagcggac gcttcgcctt cccctgacga aaccttcgag tgtcgggtcg
      181 acgggcgcgc gctggacaag tttcgccggt gatgccgacg cgccgctgct gattacgcgc
      241 aacgctggcg agcttcgccg atgcacgtcg gccagtcgaa ttgacggagg tgtttcgatc
      301 ccgtgtcgaa ctcctacgtg aggagttgcg gctcgccttc accggagggt gtgcccgcga
      361 cggctcgcga tcaccgcgcc gaattcacgt tggaggtcgg caactcggcg tcaactagcg
      421 gctagcccta tcgattcaac tgcgatacga ccctgcaaga acctacgtgt ggctccttga
      481 cgcagctcat tgcgacgcat gcctttcgaa cgacgaggtg gtgggccttg cgcgctcgca
      541 ggggacgccc ctgttcgtca tcgattgcga ccaggttcga cggcaatacg tgctcttgcg
      601 tacagcactc ccaggggtga atctacacta cgcgatcaag gcgttgccac accccgaggt
      661 ggtggcgacg ctgagggacg aggggagctc gttcgacgtc gcgagcagcg gcgagatcga
      721 gcttctgcgc tcgcaagggg tggcgcccga gcgcgctctg catacgcatc ccatcaagag
      781 cgagctcgaa atatcgcggt ctctcgcctg gggttgcaac acgttcgtgg tggacaacgc
      841 ggccgagctc caaaagttcg cgccgtttgg tgccgtggtg aagctcgttc tgcgcgtgag
      901 ctttcgcaac ccgaacgcgg ccttcgacct ctcgaagaaa ttcggttgcg cggcgcacga
      961 agcgctcgat ttgctgtgtt ttgcgcgccg cctggggctg cgggtgcgcg gcctttcgtt
     1021 tcacgtgggc tcgcagtgct cgagctccgg cgcacacgtt caggccattg gcgcttgccg
     1081 ggacctcatc ctggcggctt cggcggcggg gtttccgcca ctcgacactc tcgatatcgg
     1141 gggtggtttt ccgatctcgt actcgccaga gcccatcgat atcgacgcct tctgcgcgcc
     1201 gattcgcaag gccttggcca cgctaccccg ggggatgcgc ctgctggcgg aacccggtcg
     1261 gttcatcgtg gggcctgccg ctcacagcgt cgcctcggtg gtcgggcgtt ccgaacgtga
     1321 aggcagcctt tggtactacc tcgatgatgg cgtttacggg tcgtacagcg ggcggttgtt
     1381 cgaccacgcg ggttatccga tcagcgtttt ggcgagcgtg gcgcggcctt tgcgcgcgag
     1441 ttgtctggcc gggccgacgt gcgacagcgt ggacgtcatc gcagagaata tcctgctgcc
     1501 cgagctcgag ctcggtgatc tggtggtcgg acacaccatg ggggcttata caagcgccac
     1561 cgcgagcgag ttcaattcgt tcgagaaggc gaaggtattc gtcctgaacg caccctccgc
     1621 ggtcgtcggc gaaggtgcga cgactcacac tcgaatgctg gcagttcgca aagcagagta
     1681 gattcgacag gtgaataatc cgcgcggttg agttagagtc ctgcccgcag cgattcgagc
     1741 atcggcagtg ctggccgaaa cacgcgcgaa atgcgtcagc ggctatctac cgagaagcca
     1801 ccgtgctcgc caagaacgca aaaccagccg ttgcgaagat gtcgcgaact agccacaaag
     1861 acgtgcccgc cccgggcgcg gggaataccc cgaacgcttc ttccttcgag ccaggcatgg
     1921 tcgtcggcgg aaagtaccgg attgggtcgc tgatcggggt aggcggcgtc ggtcgagtct
     1981 atctcgcgaa gcacatcgag ctcgacaccg acgttgcgat caaggtcttg cgtccggaga
     2041 tgctgcagcg ccccgacgtc gtgcagcgct ttgggcgtga agcgcgcgcc tcggtgcggc
     2101 ttcggagcga gcgcatcgcg cgcgtgttcg acgtgggcgt cgagcacggc gtcccttact
     2161 tcgtgatgga gtgcatggtc gggtgcgact tgctcgactt tcgcctgcag aacaacccgg
     2221 acatcgcgac ggtcgcggag ctcttcatcc aggcttgtga gggcctcgcc gaggcacacg
     2281 cgcaggcgat cgtgcaccgc gacgtcaagc ccgagaacct gttcgtggtg cgcgacgaaa
     2341 acggctggcg caccctcaag atcctcgact tcggcatctc caaattctca ctcaccggca
     2401 aacactccga cgtcgatctt tcggcaaagc gcaccgaagc gatgctcggt accccgaact
     2461 acatctcccc ggagcaaatc cgcgcgaccc gcgacgtcga tcttcggacc gacctttgga
     2521 gcttgggagt ggtgatgttc gaggtgctga gcgggggtgt gctcccgttc cgcgaagagc
     2581 gcgagatgac cgcgctcatc gccgaaattc tcgagatgcc gcaccggtcc ttgctggacg
     2641 tcgctcccaa cgtgcccgag ggattggtcg ccatcgtcga tcgctgcctc gagaaagatc
     2701 gcgacaagcg ctaccaaacc gcggccgaga tcgcgctcga ccttttgccc ttcgcgccgg
     2761 tccgcgcgcg cgcctcggtc gaacgcgccg tcagcgcgat gcaagcggcc gggctcttgc
     2821 ccgtgaacct ggacctctcc tcgatgcgct cgagccaacc cccaatcgag cgcctgagcg
     2881 tctctggaat gcagaacagg ctccgctcga cgctgccgga gtcgctcgac gcggtcgaga
     2941 tcagcgaggc gaattttagg ccttccaagc gctgggtggc gccgctcgcg ctgacgttgg
     3001 tcgtggccgg tctcacctac ggcgcgacga cgctgctgcg cgagcaagcg agcgagccgg
     3061 tgaaggtgag ctcgggcgaa ccagtcaagg tgagctccgg cgagcgcatt ggcgaggcgc
     3121 ggcttccgga gccgctgctg ccgcctccgt cctcgagccc cgagccggtg gcgagcgccc
     3181 atgcgccgca atcggcgcct cgagcgcccg cgtcggctgc cgtggccacg gcgcgcccgt
     3241 cggtgtcgcc gcggccgcca gtcgccttcg cgccgagaga cttccgtccg catcccgctg
     3301 tgaattcagc tttgccggcg cccaagccgg tggtgagcac caaggccgaa gcggcgtcca
     3361 acggggctcc caagctcgac ctccgtcgtg agcgctgacc cgccctcaac tcagaaaaat
     3421 agtcgacatg tggaaacggc aagcggcgcg aacgcgcctg ggcttgatga tcttggtgtg
     3481 cgggctcggc ggtgggattg tgccctcgcg cgcgaacgcg agtgagacgg cggacgaagc
     3541 cgacgtcgag ttccagctcg gcgcggatcg ctacgacgcg ggggactaca agcaggcgct
     3601 gagtcacttc ttggcgtcga accggctcgc gccgaacaag aacgtgttgt tcgatatcgc
     3661 ccgctgctac gagcagctga agctcttgcc ggaggctttt cgctactact cgctctcgct
     3721 cgaggccgag acgagcgagg ccggcaagca tcgcatccaa gagtcgctcg aggctttgaa
     3781 accgcgcgtt gcgcgtctca ccatcgagag cgacccgccg ggcgccgtca tctatctcga
     3841 tcggaaagat cttggctcgc gcggcctcac gccacggacc ttggcgctgc cggagggcga
     3901 gcacaccgtg attctggagc tcccgggttt cgaaccgggt cgctcacgga gcgtgagggt
     3961 gaagctcggt gaggagacca aagcgaacat cgaacttacg cccattctgg gttcggtcga
     4021 ggtggtcgga acccaaggcg cggcggtgcg cctcgatagc gaagcgaacc cggtgctgtg
     4081 ctcggtgccc tgctcctttc gggctccccc gggcaaacac gtcgttttgc tctccaagcc
     4141 ggggttcggc agccgcgagg tgccggtcga agtcggggtg aaccacgccg agcgagttcg
     4201 ggcaaagctc gagccgctct ctggttcggt cgtggtgaac gccgacgtgc gcgaggcgct
     4261 cgtgtcgatc gacggccagc cgcgcgcgtt cacgcccgct gtcgtctcgg ttccggtcgg
     4321 cgagcacgaa atcgtggtgt cgcagtccgg ttttcgtccg gtgcggcgcc gcgtccgcgt
     4381 agaaaccgaa cagagcgtgc ggctcgattt cgagctttcg ggccaggaag aagtgctcgg
     4441 cgcttcgcgc aaagccgagg cggccgagga cgcgcccgcg tcggtgacca tcatttcgcg
     4501 cgaggagctc cgcgcgatgg gctacccgac gatcgccgag gcgattcgcg gcgtgcgcgg
     4561 catgtacctg tcgtacgacg atacttacta ctcgccgggt gtgcgcggct tcgcccgccc
     4621 cggagactac ggcaatcgga tcctcgtctt ggtcgacggg cacgcgacga acgacaactg
     4681 gatttggacc tcgtacatcg gcttcgacgc ccgcgtcgac atcgacgacg tcgagcgcat
     4741 cgaggtgatt cgcggcgcgg gctcggtcgt gtacggcacg ggcgccttct tcggcgtcat
     4801 caatctcgtc acgcgcagca acgatcagcc aactcacggc gaggtcgccg tctccaccgc
     4861 ccaaggcgct ggacgcgcgc gtgccacggc cgtctggcac ccacgccccg acgctggcgc
     4921 ttggctatca gttgcgggcg cgcaagcggc cgggctcgac cgctactacc ccgagtacgt
     4981 ctccgaaccg accgatgccg gcccttcgat cgtcgattac cgcggaaatc ccgccaacgg
     5041 cgtggtgcgc gactcggacg ggtttcgcgc cggcacgctc ggcgggcacg ccttttacaa
     5101 ggccctctcg acgcgctggc ttctcaccgc tcacaaaaag cactcaccga gcgcgcagta
     5161 cgaaacgctg ttcggcgacc cgcggacctc gaactcggat acgcgcggct tcgtcgacgt
     5221 cgagttcgag ccgacctccg agcacctcga gtcgctcact cgcgtccact tcgattacta
     5281 ccgctacgca aaccgactgc cctacgcgcc gaacagcgtc gatcccgcgt cgtttggtct
     5341 cgaaaacgat cgctacacgg gcgtttgggg cggcgtggag cagcgcttca cgatcaagcc
     5401 gacccaggct ttgaagctga ccgtgggcgg cgatttcacc cagcatttca cgacgcatca
     5461 gcgcaacgtc gatgaccgcg ggcgcgctgg ctacacgggc ccggacgccg gccccatcat
     5521 cgacaacaat aaccccttca agaacgtcgc gggctatgcc ttgctcgacg ccgcgatttc
     5581 gtcgcagctc cgtgtctccg ccggtgcgcg catcgattac ttcgacaagc tcgacttcga
     5641 tgcggtcgcg gcgctcagcc cgcgcgtcgc gatcatcatc aagccgtacg cccgcggtaa
     5701 cctgaaggtc atggccggca aggcctttcg cgtgccgagc gtctacgaac ggttctacgt
     5761 ctccacgacc caaatcgcgc cgacgcggat caagcccgag caggttttct cgggagaaat
     5821 cgagttcagc caccgtttct cgaacgcggt caccgggctc gtcaccggct acaccaacta
     5881 cgtgacgaac ctgattcagc tcggaacggt cacgcgcggc gatgccacct acaatcaata
     5941 cggcaactcc gacgccccag tgctcgtctt cgggggtgaa gcggaggtgc ggcgtgaatg
     6001 gcgccaaggc ttcatgctca ccgcgtccac ttctgtgcaa aaggccgagt acctgagcga
     6061 cgacaacctg cgccgggtcc cgaactcgcc gctcgtgctc ggctcggcca aagtcgtggt
     6121 tccggtcatc gggcgggcgt tggtgctcgc gtccaagctc tcgatcgaag gcgcgcgcta
     6181 cgacaaccaa taccggaacg aagacgtgac ctgcgatcca gaaggcgcgg tggtcacgcc
     6241 gtgccctcga cagcgcacga cggacaccgg catcgtgtgg gatctcgtcc tgagcggcgc
     6301 ggtcgaacga tacaacgcga actacacgct gggcgtctac aacgtgatgg actggaagta
     6361 cgacaccgtt ccgagcaccg agtacctcca acgaacgatc cgccagcgcc cgcgttcact
     6421 gctcgcgtcg ctcgctttca agttctgacc tcgcggcaag agcgcgatgg cacacgtcgg
     6481 cgtgagcgcc gctaactgcg cccgccctcc atcacgcgat ggatcgtatc cgggacgagc
     6541 ccgtcttcct cggcgatggc gacgaggcgc gcgttctgct gcagatcagt gaggaagagg
     6601 cgagcgtacg gcgtgccgac cagcgcttgg aggcgccgcg ccaacgcggg gaggccgcgg
     6661 cgaatggcgt cgcgtgcgcc ggtctcgtca ccgaggtcga ccagcgcgtc gtgcagcgcg
     6721 aggtacacgg gcgattcgtt gagcaggctc ggggagccgc gttcgagcat cgcgatggct
     6781 tcgcgtgcaa gctcggcggc ggcggacaca ttgccggaga gccgctccgc ctccgcccac
     6841 atgccgagcg cgaccggcaa gaggtcgcga tgcccggtgg tcttgtagcc ttgcgcggcc
     6901 atgcgcaata agccgagggc gcggcggtag tcgcgctcgc tcttttggcg cagaagctcg
     6961 cagcctcggt aaaagaggat gccgaggttc gcgcggtcgg gcgccgccca gatcccggag
     7021 gcggtcgcgt cggcgtcggt gcgaacttgg gcgaggtgcg catcgagcat cttgtcgctg
     7081 ccgaacgtcg cgcaccagcc gagcaggttc atctgcgcgt tgcgcatggc gccttggctg
     7141 ccaatcgcgt cggcgagcgc gagccccgag tcgagcgaag cgcgcgcctc ttgccgagcg
     7201 ccgatcgtcg tgagcgcgaa gccgaggttc gtcatcagca tcgcctcgcg ctcgcgcagc
     7261 gcggccttgt gggcggccgc gacggcgttg cgccgggact cgagggcggc tccgaccgcg
     7321 ccctgcgttt gacggatgat ggcctgcgtt tggtaggcgt cgaccgccgc ggcgctgatg
     7381 ccgcgctgtt cggcgagctg gagcaggcgc cggacttcgg cttcggcctc ggcaaagcgc
     7441 ccggcgaagg cgaggcgaga cgcgagcacg gccgagcagc gcgcttcttc gtcgtggagg
     7501 ccgagcgcgg cggcttcgtc gcgcgtttcg gcgaggcgct cgctgatgcc ggtgccggag
     7561 ccgcgcgctt cgtcgaagcg ggcgcgcgcg ccgcgagctc gcaccgcgct cgcctcgtcg
     7621 aatacgttgg cctcgagcgc catgatcgcg ctttcgcgtt cgctcgcacg cgggtcgagg
     7681 cggctccaag cttcgtcgag caacgaggct cgcacgaagc ccgagcgtcg atcctcggcg
     7741 aaggcgaggg cgcgctcggc catgttgaga gcgtcggtga gggcgttggt cgagagcgcg
     7801 cgctgggcgg cccgggtcca atgctccgcg gctgcggcgt gctgagcacc gaggtcgaaa
     7861 tgcccggcga cgaccgctgc gtcttcgccc atcgacgcga gccacgtgcc cgcgagggcg
     7921 tggagctcct tacgctcgcg ctcaccgagc gacgcatacg ccacgtcgcg cacgagcgcg
     7981 tgcttgaagg tccactcgcg cgtgcccgaa aagcgtgagt tgttttgctc gacgagcacc
     8041 tcgtgcgcga ccagcgcttt catcacgcct tcggcgcccg gcattccgaa cgcttcgagt
     8101 gcagactccc agcaggtgag gccgagcacg ctcaggcggc ccaccgcgtc gcgacattcg
     8161 tcgtcgagcg cgtccaagct cacctgaatg gcggcttgga tggtgggggc ttgcagcgtg
     8221 tcacgaccgg ccgcggtgag gcgcgcgagc tcctctgcga acaagggtaa gccgccggcc
     8281 tgttcggcta tgcggtcgag tacggcctcg ggagtgctcg ggccgagcac cgactggacg
     8341 atgctgcgcg ccgagcgccg cgagatgggc ctgagctcga gacgcacgtg atcgcgtccc
     8401 gcgaatcgct cgggattcgc cgtccaaaac tccggccgca ccatcgccat gacgagcagc
     8461 gggcgattcg cagcgcggcc catcaagtga tcgagccagc cgatactctc tggatccgcc
     8521 cattgcagat cttcgaccag aatcgcggtg ctttcgacgg ccgtgatctg catgaccaga
     8581 tcggtcatgg cgagccacaa ggcgtcgcga gagccgcgcg gatcgaggcc ttcgctcagt
     8641 ggctcgttgg cgagcaagcg cgctacgaga tcgcgggtgc gcgccgtgag ctcgtcgcgg
     8701 gtcgctggcc cgagccgagc cacgatggcg cgcgccgcct cgctgctggt cgcgcccttc
     8761 ggaagtccga tgatcgcacg cagcacgtcg gcggccgcac cgagggactg tccctggccg
     8821 taagcttcgc tccgttgaag gatgatttgc ggcgcctccg cttgcgcggc gatgcgcgcc
     8881 atcacttcgc ggcgcagacg acttttgccg atgccgggcg gccccgtgat cgtcaccaga
     8941 atcggggtgc gctccgagcg ggcgcgcgag aacgcgctca gcacttgggc aagctcggcg
     9001 tcgcgtccga cgaacggcgc tccacccgct cgatcgccgc gcccgccgcg caacggctcg
     9061 ccgacgatgg ccgaaccatc gtcgcgcgcg cgaaactcat agcggccgcg gccgagctcg
     9121 ctcgtcgtga cgtccgccag caccgtgcct ggctcggcgt cgcgcgcgag cgccgaagcg
     9181 cggtcgacga cctcgcccac cgggtgcacg acactgtcgc tcagaaggtt cacccaagct
     9241 cggccgctcg cgagcccaac gcgcgcaccc gcacgagcca gacgacgcgc gagctccaag
     9301 gcaaccaacg cctcgctccc gaccgcgcgc cgggctccga ggtgcgcgac gatcgaatcg
     9361 cgaccgagcg gcaccgcgtc cgcgccgcgt tgtcgcatcg attcgagcgc tcgttcgcgc
     9421 gccgagtcgg attcgaagcg cagcgcgacg atggacgtga tgaggcgcga accgctcgag
     9481 ccgagccgcg tcgagcgcgg ttcggagtcg aaccagctgg tgcgcgaaga atcctccgtc
     9541 gcgctccgta aaatctcgca gagctcttgc gccgagcgcg ggcgctcagc cggatccgtc
     9601 tccagcatgc tctgcacgag gctgtcgacc acgggtggta cgtcgcgccg caattcactc
     9661 aagtgcggcg agggcgtggt cgccaggcgc gcgagcgtcg cgatgacgtt cgggccaacg
     9721 tgcggaggtc gtcccgcgat gagctcgaac agcgttgcgc cgagggagta gatgtcgctg
     9781 cgcccgtcga gcggcgcgtc gccgcgcgct tgctccggcg ccatgtaggc gggcgtcccc
     9841 acgagatcac cggcgcgccg cacccgcatc tcgccgtgcg aagcgacgcc gaagtcgacg
     9901 agcttcggcc aaacgtcgag cagggctccc ggtggcttct ccagctggcg acaaaggaag
     9961 atgttgccgg gcttgatgtc gcggtgaatc acgccggctt gatgcgcggc gctcagcgcc
    10021 tcggcgacgc gcagcgtgag ctcgagcgct tccgggatcg aaagcggatt tttgcgctgc
    10081 cgagccgcga gatcttcgcc gtcgagccac tccatcgcga cgaaggggag cccggtgtcc
    10141 tcgagcacac cgaaggcgac cgtcttgacg atgccggggt gatcgaggct cgccaggagc
    10201 tct
//
LOCUS       DASTKS010000410         8450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1626766, whole genome shotgun sequence.
ACCESSION   DASTKS010000410 DASTKS010000000
VERSION     DASTKS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626766"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1834
                     /locus_tag="VFQ35_08565"
     CDS             296..1834
                     /locus_tag="VFQ35_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9930725.1"
                     /translation="MRAALPPLALDEAHGSLQQQVFECLRRAIVSGWFARGGRVPSSR
                     VLAKALGVSRNTALSAFEQLRAQGYLASRRGAGSFVATKLPDDAGLALKVISPVEPGP
                     ARSPPLAVRGRELLALLPPARRLGAAPRPFRLGTPALDLVPAALWAKIAQRRVRAIRS
                     SSLDYADPAGLKELREAIATLVRRRGTSTDADSIVVVGGAQRGIELVTQLLLDPGEPA
                     WMEEPGYPGAVHAFRVAGVRPWFVPVDEQGLVVEAGGSTCPRARLAYVSPSHQFPLGV
                     AMSLERRQALLRWAHAAGAWIIEDDYDSEFRYGGAPIHCLHGLDSQGRVLYLGTFSKT
                     LFPALRLGFLILPDGLRQAFVAARQTADVHSSSIDQLVLAEFIGEGHYEQHLRRVQAA
                     YSERLEALQEAAVRFCDGALALRPVQTGLHVVADLREVSAEVVFREACEHDLEIVPLS
                     QYYLREPNPPNALVLGFAPCRPEQLQAGMNRLSQVIEATRKLGPEARADLRALYGRAA
                     VSSE"
     gene            complement(1853..2764)
                     /locus_tag="VFQ35_08570"
     CDS             complement(1853..2764)
                     /locus_tag="VFQ35_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930726.1"
                     /translation="MDGSILSHTRYVAAELAHAFQNDPEGELTLWATTAQQREAMVVR
                     LYDSAALEARFPTTLGEPGMLNVVRKVIGGIWAQERSHTTLVQSIRILGETRMTALQT
                     LMGDIEGRLTHAATASSWLRSVASFLVGVGRTMGQAPEFTKAFQELTPRQFFRFSHEL
                     ETTAKEGYVRILELLERLESAGTADASESSSVERGLRFGVTAPYEFAKTLAEECFHAA
                     VFEQLEAWLERDGESFGGLPARDAVLRLRELAIEHLSLGSKSLGASGARLDQPWLKSV
                     GSSRLVSDGGLGELFEEYGVKLPPLLP"
     gene            2893..4020
                     /locus_tag="VFQ35_08575"
     CDS             2893..4020
                     /locus_tag="VFQ35_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET9930727.1"
                     /translation="MSRLLSLSDERIDYFRSVGDPSVDRVVEEHLGASTGELASLLAR
                     LFSTPKLPDHPLVAAYLAQLEEEPLVDPDAVTHGQQLFELYGPEVLLVLGSCALPLAY
                     AAGNGVQVVARARRLKDDPIRRLCDTAQMVINVMQPGELATGRIGWSSARKVRLVHAL
                     VRRHVRAYPASPWNPAWGTPINQEDQAGTLLTFSVGVLHGLRRMGATVSTAEGDAYVK
                     AWSAVGTLLGVDRSLLPESEAEASALAARIGARQIRKTPEGVELTTHLIAAVDSLFPI
                     SGYGASLSHFFLSDTAFGERVADVLAIPPPNWTQWLVRGRAWQKRVALSFLDRVPGAK
                     ARRSLVARHLVQRLLSWKRPDDRAPFEIPPRLQREWRLAHD"
     gene            complement(4011..5018)
                     /locus_tag="VFQ35_08580"
     CDS             complement(4011..5018)
                     /locus_tag="VFQ35_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9930728.1"
                     /translation="MKPRQLDVLRVVEAAYEFDLTNEEWLKHVAEAVRPHLDDDFGLA
                     AFEFVRTGDAPPMLLQSQHLWMPEDLAAVYPQMFANMSPELRVRPFRLGPCVTGSELV
                     NMKGEFANVPQMKNGLQRFGMFDSIWITATDPMGRGCGFHAGRKAIAWATPAEKRVWG
                     RIAAHLSSAIRLRYRLREADVREPPAAVFDPNGKLHDATGEAQEAEARAALRDAVLTL
                     EKVRGHQRTAEPEQALNDWKALVRGKWSLVDRIESDGRRYVVARENEPRAPGPAALSE
                     REKQVLGFARLGHHNKLIAYELGLAQSTVRVLMARARAKLGVETREELLRRIRELPLQ
                     S"
     gene            5329..5967
                     /locus_tag="VFQ35_08585"
     CDS             5329..5967
                     /locus_tag="VFQ35_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9930729.1"
                     /translation="MIWNGLLSGELEIIGTIAQEHCCSLTLQARTERTPPLDSRARAI
                     LERTLLGDRRKVIACEANVSASTVALVLKDSLTSIGLDCRPSRVPSSLVMLVHAACGA
                     AEPHGIFIGDCDDEGRPVTIVTHVFDANVLQGLSPSQRAILCLIASGRSNLDIAARRN
                     RSYRTVVNQIAAACLRLGVSGRLELLHRFATLPAAAAPEPQLSLSDNHHRRV"
     gene            6065..6967
                     /locus_tag="VFQ35_08590"
     CDS             6065..6967
                     /locus_tag="VFQ35_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930730.1"
                     /translation="MKTSSTERIDLLALVWLVVPRQQSRVGGFADQLKHVATGAKDRK
                     RLAEESIARLQKRGLVGPGARLELTTEGHKKALEALGVSTLPKKPSLQWAKKALLLRS
                     LGAEATSGASKKAREPGALAALILARRHRVERKHEASLTKVGAILARRALGLEEKPTI
                     KLDDVFSAVFLVNAVLAPDGTNAGHESAAPASATRALRLADCKLPEFANQVNEAARSS
                     PTGRWHGAVFISHVWNTLRHRGDVGITFENFQKRLVEAYQADLLELSRADLVEAMPAA
                     DVSASETTHLGARFHFVRLEQLAS"
     gene            6983..>8450
                     /locus_tag="VFQ35_08595"
     CDS             6983..>8450
                     /locus_tag="VFQ35_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930731.1"
                     /translation="MSTTNDQLNAFLTERTEVFRSVEDRQQIWIDDPFDTSTLHAEAR
                     DVFTSLVADVTGPDCPTSGRILLILGDAGAGKTHLIRSFRSYVHKSRAGYLGYLQMAS
                     GASNYARYVLSNLIDSLDQPYDQEVDSRTSLTRIAGELAARAFQAHVASSLRDDEDFE
                     DQELAEQVRIGADHLLKTRYPELDIDLLRAFLYLHRPDPRIKSRILKYLRCEDLSQND
                     RQRIGDIVPKTDANDPERMVRSIGRLLAAVGETPMSLVLCVDQLEDAFSTERSEQPFI
                     RAMNTLRELAEGLPTAIIVICCLRNLYEELRPKLSEALRARIEKDPEPVRLRAGVTAE
                     EGKALVGERLRYLYERAGLPVNGKADIYPFTQNFFAQRHRISARQLLDACREYRDRCR
                     KAGKLVELDVGVEPPPPPSVRENEQAKRTAEWERKWNDFLAESHDSPPDEDEELASAL
                     CWAITCAGAELKHSGSSVEVTQEESALSVRLKTAGAPDE"
BASE COUNT         1509 a         2735 c         2663 g         1543 t
ORIGIN      
        1 gcttccatcg agcggctgat cccatgccac gagtcccgcg ctgaagctcc cgacccagtg
       61 gatcggctgc ttgcgagcgt cctcggccgc tacgcgggtc gcacccagag ctcccaacga
      121 caaagcgacc aaaccccaaa ccaaaaccga acaacgccta gcattcatct tcttcgacct
      181 ccgcccgttg atgtccggca gggcgaaagc acatggatcc acttcgcgaa caacagagga
      241 ggccacttcg tccggcccgc ttgaccggcc cggatttccc ctacaaggaa gggcaatgcg
      301 ggcggcgttg cctcctcttg cgctcgacga agctcacgga tcgcttcagc aacaggtttt
      361 cgagtgcctg cgccgcgcca tcgtgtccgg ctggttcgct cgaggcggtc gtgtgccttc
      421 ctcgcgcgtg ttggcgaagg cgctcggggt gtcgcgcaac acggcgctct cggcattcga
      481 gcagctgcga gcgcagggct atctggcttc tcgccgtggc gccggcagct tcgtcgcaac
      541 gaagctcccc gacgacgccg gcctcgcgct gaaggtcatt tcccccgtcg agcccggccc
      601 agcgcgctcg cccccgcttg cggtgcgagg gcgtgagctg ctcgcgttgc tccctcccgc
      661 gcgtcgtttg ggagccgctc cgcgcccatt tcgtttgggc acgccggccc tggacttggt
      721 gcccgcggct ctctgggcca agattgccca acgccgggtt cgcgcgattc gctcgtcttc
      781 gctcgactac gcggatccgg cggggctgaa ggagctgcgg gaggcgattg ctacgctggt
      841 tcgccgacgc ggcaccagca ccgacgcgga ctccatcgtc gtcgtcggcg gagcgcaacg
      901 ggggatcgag ctcgtcactc agctgctgct cgatcccggt gaaccggcct ggatggagga
      961 gcccggctac ccaggcgcgg tccacgcctt tcgtgtcgcc ggcgtgcggc cttggttcgt
     1021 gccggtggac gagcagggct tggtagtcga agccgggggc agcacgtgtc cacgcgcgcg
     1081 gctcgcctac gtgtcgccgt cgcaccagtt tccactgggt gtggcgatga gtctcgaacg
     1141 acgccaggcg ctgctgcgct gggcgcacgc ggcgggcgca tggattatcg aggacgacta
     1201 tgactccgag ttcaggtatg gaggcgctcc gattcattgc ctgcacgggc tcgatagcca
     1261 ggggcgtgtt ttgtacctcg ggaccttcag caagacgctc tttcccgcct tgcgactggg
     1321 ctttttgatc ttaccggacg gcttgcggca ggcgttcgtg gcggcacgac agaccgccga
     1381 tgttcactct tcgagcatcg accagctggt gctcgccgaa ttcattggcg agggacacta
     1441 cgagcagcac ttgcgccgcg ttcaggccgc gtacagcgaa cgcctcgagg cgctgcaaga
     1501 ggcggccgtg cgcttctgcg acggcgcgct cgccttacgc ccagtccaaa ccggcctaca
     1561 cgtggtggca gatctgcgcg aggtttctgc agaggtcgtg tttcgagaag cgtgtgaaca
     1621 cgatctcgag atcgtaccgc tgtcacagta ctacctgcga gagcccaacc caccgaatgc
     1681 gctcgtgctg ggcttcgctc catgccgccc cgaacagctc caggcgggga tgaatcgtct
     1741 cagccaagtg atcgaggcga cgcgaaagct gggcccagag gcgcgcgcgg acttgcgagc
     1801 gctttacggc cgcgctgccg tgagctcgga gtgatacctg cgcgtcgtgc gctcatggca
     1861 gcagcggagg cagcttgacg ccgtactctt cgaagagctc gccgaggccg ccgtcactca
     1921 cgaggcggga gctgcccacc gacttgagcc aaggttggtc gagccgagcc ccgctcgctc
     1981 ccaagctctt ggagccgagc gaaaggtgct cgattgcgag ctcgcgcagg cgaagcactg
     2041 cgtctcgcgc gggaagacct ccgaagcttt ctccgtccct ttccaaccag gcctcgagct
     2101 gctcgaacac cgccgcgtgg aagcactctt cggcgagcgt cttcgcgaac tcgtaaggag
     2161 cggtgacacc aaacctcaag ccgcgttcga ccgagctcga ctcgctcgcg tcagccgtac
     2221 ccgcggactc gaggcgttcg agcagctcga gtattcggac gtagccttct ttcgccgtgg
     2281 tctcgagttc gtgagagaac cgaaagaatt gtcgcggtgt gagttcctgg aacgccttgg
     2341 tgaactccgg cgcctgcccc atcgttcggc ccacgccgac caaaaacgaa gcgaccgatc
     2401 tgagccagct gctcgccgta gcggcgtgag tcaatcggcc ctcgatatct cccatcaacg
     2461 tctgcagggc ggtcatgcgc gtctccccta gaattcggat cgattgcacg agggtggtgt
     2521 gagagcgttc ttgcgcccaa atcccgccga tcaccttcct tacgacgttc aacatgcccg
     2581 gctcgccaag agtcgtgggg aagcgggctt cgagcgcggc agagtcgtag agccgaacga
     2641 ccatggcttc gcgctgctgc gcggtggtcg cccagagcgt gagttcgcct tcggggtcgt
     2701 tctggaaagc gtgcgcgagc tcagccgcaa cgtagcgcgt gtgagagagg atcgagccgt
     2761 ccatgcatca ttcctttctc gaacggtacg gctccgcggc acatcccgcg aatagtaaaa
     2821 aatcctaaag ctcggagtca gcccgctcga cgcttgcaga cctcggccgc gaaatgacaa
     2881 cttgggcgga ccgtgtcgcg acttctttca ctcagcgacg agcgcatcga ctatttccgc
     2941 agcgtcggag acccgagcgt cgatcgtgtc gtcgaagagc atctcggagc gtccaccgga
     3001 gagctcgcgt cgctccttgc tcggctcttc agcacgccga agctaccgga tcatccgctg
     3061 gtcgcagcgt atctcgcgca gctcgaggag gagccgctgg ttgatccaga tgcggtgacc
     3121 cacgggcagc agctgttcga gctctacggg ccggaggtgc tgctcgtgct cggctcgtgt
     3181 gcgctgccgc tcgcatacgc ggcggggaat ggagtccaag tcgtcgcacg cgcgcgaagg
     3241 ctgaaagacg atccgattcg caggctttgc gacaccgcgc agatggtcat caacgtcatg
     3301 cagccaggtg agctcgccac gggacgtatc ggctggtcgt cggctcgaaa agtgcgtctg
     3361 gtccacgctc tcgtgcgccg acacgttcga gcgtacccag catcgccgtg gaacccagcg
     3421 tggggaaccc caatcaatca agaggatcag gcgggtacac tgctcacgtt ctcggtcggt
     3481 gtgctccacg gcttgcgccg tatgggagcg acggtttcga ctgccgaggg cgacgcgtac
     3541 gtgaaggcgt ggagcgccgt gggcacgctt ctcggcgtcg accgctccct cttaccggag
     3601 tcggaagccg aggcatcggc gctcgcagcg cgcatcggcg ctcgacagat tcgcaagacg
     3661 cccgaaggcg tggagctgac gacgcacctg atcgccgccg tcgacagctt gtttccgatt
     3721 tcgggatacg gcgcgagcct gagtcacttc ttcttgagcg acacggcgtt tggtgagcgt
     3781 gtcgccgacg tgctcgccat cccgccgcca aactggacgc agtggctcgt gcgcgggcgc
     3841 gcctggcaga agcgagtggc gttgagcttt ctcgaccgcg tccccggggc gaaggcgcgg
     3901 cgcagcctcg ttgccaggca cctggtccaa cggctcttgt cgtggaagcg acccgatgac
     3961 cgcgcgccct tcgagatccc accgcggctg cagcgtgagt ggcggctggc tcacgactga
     4021 agcggaagct ctcgaatgcg ccgcaggagc tcctcgcgcg tctcgactcc gagcttggcc
     4081 ctcgcgcgag ccatgagcac gcgcacggtc gattgggcga gaccgagctc gtaggcgatg
     4141 agcttgttgt gatgaccgag gcgagcgaag ccgagcacct gcttttctct ttcgctcaat
     4201 gcagccggtc cgggcgcgcg cggctcgttc tcacgggcca cgacgtaacg gcggccatcg
     4261 ctctcgatgc ggtcgacgag cgaccacttg cccctgacga gagccttcca gtcgttaagc
     4321 gcctgctcgg gctcagcggt gcgttggtgg ccgcgaacct tctcgagcgt caacaccgcg
     4381 tcgcgcaagg cggctctcgc ctccgcctcc tgcgcctctc cagttgcatc gtggagcttg
     4441 ccgttcgggt cgaacactgc agccgggggt tcgcgcacat ctgcttcgcg caggcggtat
     4501 cggagccgga tcgcgctcga aagatgtgcc gcgatgcgcc cccacacccg cttctccgct
     4561 ggcgttgccc acgcgatcgc cttgcgcccg gcgtgaaatc cgcaaccgcg acccatggga
     4621 tccgtagcgg tgatccagat tgagtcgaac atgccgaacc gctgcagccc gttcttcatc
     4681 tgcgggacat tcgcgaactc ccctttcatg ttcacgagct cactcccagt gacgcacggg
     4741 ccgagccgaa atgggcgtac ccgaagctcc ggcgacatgt tcgcgaacat ctgaggatac
     4801 accgcggcca gatcctccgg catccaaaga tgctgactct gaaggagcat cggaggagca
     4861 tcacccgtgc gcacgaactc gaacgcagca agcccgaagt catcgtcgag gtgtggacga
     4921 accgcctcgg cgacgtgctt gagccattcc tcgttcgtta agtcgaactc gtacgcggcc
     4981 tccactacgc gcaggacatc caactgcctg ggcttcattc gacccccgaa gataacccaa
     5041 tcgtgtcgca ccaccagcgc tcttcgtggt gcctcctcga aacgcaacat ttttcgcaga
     5101 aactcgagct tacaagggac ttgtaagctc ctccgacgtt gaacttcgag tcgagtcggc
     5161 gcacgctgag catcagctgc agacagcgac tgcaagctgc aaccaatcat cgtgttgtgg
     5221 cgaggctgtg tgcgcgcggt ggtgcgtatg acgtcgcaag acctacatcg catgggtata
     5281 ttggcgcaac gcgtcgaatc ggcagccgct tcaaacgagc gctgttcagt gatttggaac
     5341 ggcctacttt cgggcgagct cgaaatcatc gggactatcg cgcaggagca ctgctgctcg
     5401 ttgaccttgc aggcacggac cgagcgcacc cccccgctcg actcacgcgc tcgggcaatt
     5461 ctggaacgga ctcttctcgg tgaccggcgc aaggtgattg cgtgcgaagc caacgtatcc
     5521 gcttcgacgg tcgcgctcgt gctgaaagat tcactgacga gcatcggact cgactgccgg
     5581 ccttcgcgag tcccttcatc gctcgtgatg ctggttcacg cggcgtgtgg cgcggctgaa
     5641 cctcacggca tcttcattgg cgattgcgac gacgaggggc gacccgtcac gatcgtgact
     5701 cacgtattcg acgcgaacgt tctgcaaggg ctctccccga gtcaacgcgc gatcctgtgt
     5761 ctgatcgcca gcgggcggag caacctggac atcgccgcgc gtcgcaaccg ctcttatcga
     5821 accgtagtca accagatcgc tgcggcatgc cttcgactcg gtgtctccgg gcgcctcgag
     5881 ctcttgcacc gcttcgcaac gctgccggcg gccgcagcgc ctgagccgca acttagcctt
     5941 tccgacaatc atcatcgtcg agtttgagaa aacctgaata cgctggttcg cgatcggcag
     6001 aggagccaac gcgcaggggc gcgactctgg ctaatcgtcg agcagctggt ccgagggaga
     6061 gaagatgaag acgtcttcaa ccgaacgtat cgacctactg gcattggtct ggctcgtcgt
     6121 gccgcgacaa cagagccgag tggggggctt tgctgaccag ctcaagcacg tggcgaccgg
     6181 agccaaggat cgcaaacgac ttgctgaaga gagcatcgcg cggcttcaaa aacgcgggct
     6241 ggttggaccg ggcgcgcggc tcgagctcac gacggaaggg cataagaaag cgctggaggc
     6301 gctcggcgtt tcgacgcttc cgaagaagcc ttcgctgcaa tgggcgaaga aggctttgtt
     6361 gctgcggagc ctgggcgcgg aggccacgtc gggagcctcg aagaaagcgc gcgagccagg
     6421 cgccctcgcc gcgctcatcc ttgcgcgccg acatcgggtg gagcgcaaac atgaggcgtc
     6481 gctgaccaaa gtcggggcga tcctggctcg ccgggcgctc ggcctcgaag agaagcccac
     6541 catcaagctc gacgatgtgt tttcggctgt ctttctagtg aacgctgtgc tcgcgccgga
     6601 cggaacgaac gccggtcacg agagcgccgc accggcatcg gcgacccgcg cgcttaggct
     6661 cgccgactgc aagctcccag agtttgcgaa ccaggtgaac gaagccgcac gttcctcgcc
     6721 gaccggtcgc tggcacggcg ccgtgttcat ctcgcacgtt tggaacacgc tgcgacatcg
     6781 cggcgacgtc gggatcacgt tcgagaattt tcagaagcgc ttggtcgaag cctaccaagc
     6841 agatctgctc gagctcagtc gcgctgatct ggtggaagcc atgccggcgg cggacgtttc
     6901 cgcctccgaa acgacccatt tgggcgcgcg gtttcacttc gttcgtctcg agcagctcgc
     6961 cagctgagga ttggattcgt gcatgtcgac gacaaatgac caactgaacg cgttcctgac
     7021 ggaacgaacc gaagtctttc gttcggtcga agaccgccag cagatctgga tcgacgaccc
     7081 tttcgatacc tcgacgctcc acgccgaagc gcgtgacgtt ttcacgtcgc tggtggcgga
     7141 cgtgacgggc ccggactgcc caacctcggg gcgcatactc ttgatcctgg gtgatgccgg
     7201 agctggcaaa acacacttga ttcgctcgtt tcgcagctac gttcataagt cacgcgcggg
     7261 ctacctcggt tacctccaga tggcgtccgg agccagcaac tacgcgcgct acgttctcag
     7321 caacctgatc gattcgttgg accagccgta cgatcaggaa gtcgattctc ggaccagcct
     7381 gacccgcatc gccggtgagc tcgccgcgcg agctttccaa gcccacgtcg cgagctctct
     7441 gcgcgacgac gaagacttcg aagatcagga gctcgccgaa caggttcgca ttggcgcgga
     7501 tcacttgttg aagacccggt atccggagct cgacatcgat ctgctgcgcg ctttcctcta
     7561 cctccaccgg cccgacccgc gcatcaagag ccggatcctg aaataccttc gctgcgaaga
     7621 cctgtcgcag aacgatcgcc agcggatcgg tgacatcgtc ccgaagacgg acgcgaacga
     7681 ccctgagcgc atggtacgga gtatcgggcg cttgttggcg gcggttggag agactcccat
     7741 gtcgctggtt ctgtgcgtcg accagctgga agacgctttc tcgacggagc gctccgagca
     7801 gccattcatt cgcgccatga acacgctgcg agagctggcc gagggactgc ccaccgcgat
     7861 catcgtcatc tgctgccttc ggaacttgta cgaagagcta cggcccaagc tttcggaggc
     7921 actccgggct cgcatcgaaa aggatccgga acccgtgcgg cttcgcgccg gcgtgaccgc
     7981 ggaggagggc aaagcgctcg tcggtgaacg gctgcgctac ctctacgagc gggctgggtt
     8041 acccgtgaac ggcaaggccg acatttatcc gttcactcag aacttctttg cgcaacggca
     8101 tcgcataagc gctcgacagc tgctcgacgc ttgccgcgaa taccgcgacc gatgcagaaa
     8161 agctggcaaa ttggtcgagc tcgacgtggg cgtcgaaccg cctccgcctc cctcggtgcg
     8221 tgagaacgag caagcgaagc gcaccgcgga gtgggagcgc aaatggaatg acttcctcgc
     8281 ggagagtcat gactctccgc ccgacgagga cgaggagctc gcgagcgcac tgtgctgggc
     8341 gatcacctgc gccggggctg agctgaagca ttcgggaagc tcggtggagg tgacgcaaga
     8401 ggagagcgct ctttccgtcc gactcaagac cgcgggagcc cccgacgagt
//
LOCUS       DASTKS010000411         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_957250, whole genome shotgun sequence.
ACCESSION   DASTKS010000411 DASTKS010000000
VERSION     DASTKS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957250"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFQ35_08600"
     CDS             <1..1177
                     /locus_tag="VFQ35_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9930732.1"
                     /translation="AHYGPSPLEAHVPDELRGELFGAAYWQWVGLLVAVLGALLLGRA
                     FAYVAYRVGQRVATRTRVLWDDELVRALRSPTRLFMSVFAFVPLAQLLSLPGNVRLVC
                     IHIVRTLGITALAWALTRVVAVVSNVVERRATLNAAEDARFSVRTAQTQVRVLRRVAN
                     LIVAVCGVALAAVQFEVVRNVGVSLLASAGIAGVVLGFAAQRTLGSIFAGIQLSITQP
                     IRIGDDVLVENEFGTIEEITLTYVVVRVWDERRLIVPMTRFLEQPFQNWTKISPRLHG
                     TVLLQADFTLPVDALRAELERILQGNPRWDGRTKAVHVTDARERTLEVRVLVSGEDSG
                     KLFELRAEVREKLIAWLTAFEDGRYLPKTRFAGSEKHGEAELDAMRGAADALNHGSA"
     gene            complement(1196..2179)
                     /locus_tag="VFQ35_08605"
     CDS             complement(1196..2179)
                     /locus_tag="VFQ35_08605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET9930733.1"
                     /translation="MPRDDQSAFAQSVEPLRPAIRLHCYRMLGSSHDGDDVVQETLLR
                     AWRAKDSLEDPALLKPWLYRIATNVCFDELKRRPKRAYPTDAFPPTADAKPPLPRIDE
                     PVWLEPMPNTWLEGVDERDPQASYDLKESVALAFVAALQCLSPVQRATLLLRDVVGLS
                     ASETANALGGSVDGANSALFRARSALSDRLGGDAPLDIAAQSEVDEGVLERYVRAFEE
                     ADLDALVALFHDEMRTTMPPAPTWVSGRSANEQFYRKMFGTLVAGQFRHLRIGANGKP
                     ALAFYRPAAPGDVHQLAAIQLVTTRAGAVFAVDHFMLAEVYPLFGVPRELT"
     gene            complement(2169..2822)
                     /locus_tag="VFQ35_08610"
     CDS             complement(2169..2822)
                     /locus_tag="VFQ35_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9930734.1"
                     /translation="MKLYDFAYSPNSRKVRAVAHELRIALESCHVDLLAGGSRTPAFL
                     AINPNGRVPVLVDGDFVLWESTAIIRYLSSKRDGALVPTSARGQAEMDRWICWQLAHL
                     GPAMSKVAFERIVKRLTGQGPADEAAILAGSAEFAKLTAVLESALQRRGREAEYLAGE
                     LSLADFALASHYSLAAACGLELGRFERVSAWLARVLERESMKRALAEAQATVRSNAA"
BASE COUNT          545 a         1062 c         1037 g          548 t
ORIGIN      
        1 cgcgcattac gggccgagcc cgctcgaagc gcatgttccg gacgaacttc ggggagagct
       61 cttcggcgcg gcgtactggc aatgggtcgg cttgctggtg gccgtgctcg gcgcgctgct
      121 cttgggccgc gccttcgctt acgttgcgta ccgtgtcggt caacgcgtgg ccacgcggac
      181 gcgcgttctg tgggacgacg agctcgttcg cgccctgcgc tcaccgactc ggctgttcat
      241 gagcgtcttt gcgttcgtgc cgctcgcaca gctcttgtcg ctgccgggga acgtacggct
      301 cgtttgcatc cacatcgtgc gcacgttggg cattaccgcg ctcgcgtggg cgttgacgcg
      361 cgtcgtggcg gtcgtatcga acgtggtcga gcggcgagca acgttgaacg cggccgagga
      421 cgcgcgattc agcgtgcgca cggcccaaac tcaggtgcgt gtgcttcggc gggtcgcaaa
      481 cctgatcgtc gccgtctgcg gcgttgcgct cgccgccgtg cagttcgagg tagtgcgaaa
      541 cgtcggcgtc tccctgctcg cgtccgccgg aatcgcgggc gtggtgctgg ggttcgcggc
      601 gcaacggacg ctcggctcca tcttcgctgg cattcagctc tcgatcacgc agcccattcg
      661 aatcggagac gacgtgctcg tcgaaaacga gttcggcacg atcgaggaga tcaccctgac
      721 gtacgtcgtc gtgcgcgttt gggacgagcg ccgcctcatc gtgcccatga cccgctttct
      781 cgagcaaccg ttccagaact ggacgaagat ctcgccgcgc ttgcacggca cggtgctact
      841 tcaggccgac ttcacactcc cggtcgacgc gttgcgcgcc gagctcgagc gcatccttca
      901 ggggaatccc cgctgggatg ggcgcaccaa agccgtccac gtcacggacg cgcgcgaacg
      961 gacgctcgag gtgcgggtgc tggtttcggg agaagacagc ggcaagctgt tcgaactccg
     1021 tgccgaggtg cgcgagaagc tcatcgcttg gctcaccgcc ttcgaggacg gacgttactt
     1081 accgaagacg cgcttcgccg gctcggaaaa gcacggcgaa gcggagctcg acgcgatgcg
     1141 cggcgccgca gacgcgctga accatgggag cgcctgagga gccgcgacct cacgctcaag
     1201 tgagctcgcg cggcactccg aagagcggat agacttccgc gagcatgaag tgatcgaccg
     1261 cgaacaccgc gcccgcgcgc gtcgtaacga gctgaattgc agccaattgg tgaacgtcgc
     1321 cgggcgccgc cggccggtag aaagcgagag cgggcttgcc gttcgcgccg atacgaaggt
     1381 ggcgaaattg accggcgacc aacgtgccga acattttccg ataaaattgc tcgttcgctg
     1441 agcgccccga cacccaagtc ggcgccggcg gcatcgtcgt ccgcatttcg tcgtgaaaca
     1501 gcgcgaccaa tgcatcgaga tccgcctctt cgaaggcgcg cacgtagcgt tcgagcacgc
     1561 cttcgtccac ctccgactgg gcggcgatgt cgagcggagc gtcgccgccg agccggtccg
     1621 aaagggcgct gcgcgctcga aacagcgcgc tgttcgcccc gtccacgctc ccgccgagcg
     1681 cgttcgcggt ctcgctcgcg gaaagcccga ccacgtcgcg cagaagcaac gtggcacgct
     1741 gaaccggcga caggcactgc aacgccgcca cgaacgcgag cgcgacgctc tccttcaagt
     1801 cgtagctcgc ttgagggtcg cgctcgtcga ctccttcgag ccaggtgttc ggcatcggct
     1861 cgagccaaac cggctcgtcg atgcgcggca acggtggctt cgcgtccgcg gtcggaggga
     1921 acgcatcggt cggatacgct cgcttcggtc gccgtttcag ctcgtcgaag caaacgttcg
     1981 tcgcgatgcg gtagagccag ggcttgagca gcgccggatc ttcgagcgag tctttcgcgc
     2041 gccaggcccg gagcagcgtc tcctgaacga cgtcgtctcc gtcatgggac gaaccgagca
     2101 tccgatagca atgcaggcgg atcgccgggc gcagtggctc gaccgattgg gcgaatgcag
     2161 attggtcgtc acgcggcatt ggacctcacc gttgcttgtg cctcagcgag cgcacgcttc
     2221 atactttctc gctcgagcac gcgcgcaagc caagcgctca ctcgctcgaa ccggccaagc
     2281 tccaagccgc acgccgcagc caggctgtag tgcgaagcga gggcgaaatc agcgagcgaa
     2341 agctcccccg ccaaatactc ggcctctcgt ccgcgccgtt gaagcgccga ctcgagcacc
     2401 gccgtgagct tggcgaactc ggcgctgcca gccagaatag ccgcctcgtc cgcggggccc
     2461 tgtccggtga gtcgcttcac gatccgctcg aaggccacct tgctcatcgc ggggcccaag
     2521 tgcgcaagct gccagcagat ccagcgatcc atttcggctt gcccgcgagc gctcgtcggc
     2581 accagcgcgc cgtcgcgttt cgacgagagg tagcgaatga tcgccgtcga ctcccacagc
     2641 acgaagtcgc cgtcgaccaa cactggcacg cgaccgttcg gattgatcgc cagaaaagcc
     2701 ggcgtgcgcg accccccggc tagcaaatcc acgtggcacg actcgagcgc gatccggagc
     2761 tcgtgggcca cggcgcgcac cttccgggaa ttgggtgaat acgcaaaatc gtacagcttc
     2821 atggtgtctc tccgggttcg acgcggccac gatggccacg cgcttctccc ttcaagactg
     2881 cggagggatc gaaaactgtt cgcgaccccg tcgattttcg aagactctcg ccaacccgtc
     2941 gcaacaattg atcttttaca caagccgcga caacgcgagg ccggaggatt ctcgcccccg
     3001 gcctcgctca tcagagccta gggcgtgcct tggccggaca gtagcgtgag cgtgtagggc
     3061 agcggccttc tgccgaccgc gtcgctgaac tgaaggcgct gggttagcga ctggccgggc
     3121 tcgaggacgc cgctcgggag gaacagacga atgtacgggt ctccgccgtg ggtcatgccg
     3181 ctcgcgttct tg
//
LOCUS       DASTKS010000412         6881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_287452, whole genome shotgun sequence.
ACCESSION   DASTKS010000412 DASTKS010000000
VERSION     DASTKS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287452"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFQ35_08615"
     CDS             <1..915
                     /locus_tag="VFQ35_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9930735.1"
                     /translation="PGSEGYALRVPIVQGEFALAHLCRLVGALEIPSAALKARLPAGT
                     DVELTLELDRGGQLRATARIAGVDGLFDRVAMLVTPKLSLEAMTELHADLKERAGNLS
                     RTAFQTGASKVLSQLSQALPALDDAARNLAAARGGDLDAGEQARRALTEVDALLAQIE
                     AERAWPELEERLDTDYATALTWIAEFGTEAERATLDKAAQTARRALVAHSADEVERQL
                     SLIRRLRTAAYLRHPDAWEWEFGYWEARVAELTDFKRGTELVTQGRAALANRRRDQLE
                     KVVRELWKLGPVDREEQAQSYGSGLRSR"
     gene            912..1262
                     /locus_tag="VFQ35_08620"
     CDS             912..1262
                     /locus_tag="VFQ35_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930736.1"
                     /translation="MSTHGPLDAWRRNPFFVLGVPPTATRAELERSGQKLLALLAVGN
                     AAAKHCETPLGPLERDADLVRNALAELRDPRTRIAHELWATLAQSPITTEPVEPGWEQ
                     ATSAISVRGPWSIR"
     gene            1247..2683
                     /locus_tag="VFQ35_08625"
     CDS             1247..2683
                     /locus_tag="VFQ35_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930737.1"
                     /translation="MVYPVAWAAIILVRLLFAKQSTTQREAQRSGRVLLLKFLALSFG
                     ISFLIRMGPWAVPFGLVLALYMLPGLVLNCVLVPLGWPRVAYYYARIAWPLGFSDSDA
                     VVASYYAVRALHSKSDAQVFAKVQKAFSKAKPKDMNGGAVVTLGLVALANGEPEQARQ
                     LFHVAHALNPRFISRGTRALARDYLVVDAVRSGAYRTAIRLGTTRKPTLRWAYFVARA
                     SQRLIDDPAAPSNFKLWIYYLVAPRRRVTYSLLRRALDRSALAPSIEQAAPDLASALA
                     TLAATLRDSPTTNTARLNAVVSRVEAALTDPKTHALIERRLLGLNSNESVEAVLSRFR
                     AELVDRLVPLIERSDELDGSAELTPVLEEAFHEARRGAFRELSALCKDYRKRTEEETG
                     LDTDVEWKLWANFRATAERVLRIDPQAGQEVFAEAFIPICNFAVLQHNTYKALALAHS
                     MYAWLHRHADWSPKDSLLLAKNMKAAAS"
     gene            complement(2739..3347)
                     /locus_tag="VFQ35_08630"
     CDS             complement(2739..3347)
                     /locus_tag="VFQ35_08630"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9930738.1"
                     /translation="MFNTDPRTHSVASESAGATSVGPLVNGIDTTAFMAAGAAMVSDP
                     SLAPVHFSAKSTWQGGLRSQTEIESYQLGERTIARRHVIRSDEPNEILGSNTAPNPQD
                     LLLAALNACMLVGFVAAATKRGIEVQHLCIQSSLGFDLRGAFGLDASLKPGAELIRYR
                     LEVKAKASPEQLAEVHEEMKMTSPNRWHLSQPMRFDAEFVVR"
     gene            complement(3383..4312)
                     /locus_tag="VFQ35_08635"
     CDS             complement(3383..4312)
                     /locus_tag="VFQ35_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930739.1"
                     /translation="MSRSDAPVWARTLALSERAAFRLDDVLAEDASFDFARPFLPNAL
                     TAVDSLDFLNTRERLLLGHIRAHGYLGLFGIVEEFIVPFVLAQAKPRGADLDALRALV
                     GFAGEETKHIALFRRFLRVFERGFGARPALIGPASDFSSAVLAHSELGVALAILHIEW
                     MTQRHWSDCVRADETLEASFKKLLRAHWMEEAQHARLDAQLVAELVSSATASARSEGV
                     SDYLDILRSMDDALRVQVELDLVSFELASARRLSPEQRAEFLRVQQASQRGCFLLAGI
                     THPEVLIALSGVAPDALERVARAAESFSREQPN"
     gene            complement(4299..4649)
                     /locus_tag="VFQ35_08640"
     CDS             complement(4299..4649)
                     /locus_tag="VFQ35_08640"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HET9930740.1"
                     /translation="MNHVCLQVTFALKVSSEEWLSHCERIAPRTATVPGLIWKLWLLS
                     ADGHTAGGLYLFRDLASARSYAEGPVLAGLDGSGLVEQLEVRFQSVLDAPSRRTRGLP
                     ELSLSGAGAPGVTI"
     gene            4735..6339
                     /locus_tag="VFQ35_08645"
     CDS             4735..6339
                     /locus_tag="VFQ35_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930741.1"
                     /translation="MRNPKTRLSERLARSNREALFGRERELALLEAQLAPEGAVVTFV
                     HGAGGIGKTSLLAAAMPRLEELGALVVTLDARSIAPTARAFLAAVRAKVGVEPFGVHD
                     LQEEIRDAEDIRALAQVLEREPAPHAFLVDEFDNLRLLDDWLRREFLPELPERTRWVF
                     AGRFGPRAAWLTTPGWSRAVVSLKLEPLDEAAARTLLLARGVPEEEVARLTRLARGTP
                     LALTLVTAKSEAEPTVNENAVLAALAERSVQALPLALREALEAASVVRRVTRDMLVEL
                     LGAAYDESLLDELSQLSFVEHAEDGLVLHETVRHAVASRLRALDPSRHQRLRQSASAV
                     IARALDSQPKPRSGAWRLLADMLFLSDEPGLREAFFPTRDNGAAVMDAALASDHAPLR
                     ALVERNDEGPWLEVFEAWWQHAPKCFRVVRGSHGALLGFSLVVPSGDIPRALTSEDPL
                     LGTWQADLAASPGGERGALFMRRALAAQGETEEATRSLIWLDVKRSYVERPEQWALYV
                     ATREAERRALRPCASRVSTRGARARG"
     gene            6317..6808
                     /locus_tag="VFQ35_08650"
     CDS             6317..6808
                     /locus_tag="VFQ35_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9930742.1"
                     /translation="MALEHAGEATIQLRFGEKGVWGWLRDLIQGSNRDLFQGDNRDLV
                     QGSNSPELRSVEPGDPRGTSVPSWHLDEAQRALVIVGERQPLSALEYRALSYLLEQAG
                     RVVTREQLLAAAWEQSYSGSNVVDALIRLLRKKLGPFAGDLETVRGHGYRLSRSGRRA
                     DVT"
BASE COUNT         1207 a         2229 c         2269 g         1176 t
ORIGIN      
        1 cccggcagcg aaggctacgc tttgcgagtc ccaatcgtgc agggtgagtt cgcgttggcg
       61 cacctctgtc gcctcgttgg ggcgctcgaa atcccgagcg cggcgctgaa ggcgaggctg
      121 ccagcgggga ccgacgtaga gctgacgctc gagctcgatc gcggcggaca gctgcgggcc
      181 acggcacgca tcgctggtgt cgatggcctc ttcgatcgcg tagcgatgct cgtgactccc
      241 aagctctcgc tcgaggccat gaccgagctc catgccgatt tgaaggagcg cgcgggaaac
      301 ctttcgcgta cggcgtttca aaccggcgcg agcaaagtct tgagccagct ctcgcaggcc
      361 ctgccggccc tcgacgacgc agcgcgaaac ctggccgccg cgcgcggtgg cgacctcgac
      421 gccggcgaac aagctcgccg cgcgctcacc gaggtggatg cgctcctcgc gcaaatcgag
      481 gccgagcgcg cttggccgga gctcgaagag cggctcgaca ccgactacgc cacggcgctg
      541 acgtggatcg ccgagttcgg caccgaagcg gagcgcgcga cgctagacaa ggccgcccaa
      601 accgctcgcc gggcgctcgt ggcccacagc gccgacgaag tcgagcggca gctctcgctg
      661 attcgtcggt tgcgtacggc ggcatacctg cgtcatccgg acgcttggga gtgggagttc
      721 ggctattggg aggcgcgcgt cgccgagctc accgacttca aacgcggaac cgaactcgtc
      781 acgcaaggcc gggccgccct cgcgaaccgt cgccgcgacc agctcgagaa agtcgtacgc
      841 gagctctgga agctcggccc cgtcgaccgc gaggagcaag cgcagagcta cggctcgggc
      901 ttgaggtcgc ggtgagcacc cacggtccgc tcgacgcttg gcgacgaaac ccctttttcg
      961 tcctcggagt accgccgact gcgacgcggg ccgagctcga acgctccgga caaaagctgc
     1021 tcgctctgct cgcggtcggc aacgccgcag cgaagcactg cgagaccccg ctcgggccgc
     1081 tcgagcgcga cgcggatctg gtacgtaacg ccctcgccga gcttcgcgat ccgcggacgc
     1141 gtatcgcgca tgagctctgg gcaacgctcg cccaaagccc aatcacgacc gagccggtcg
     1201 aacccggctg ggagcaagcg acgagcgcga tcagcgtgcg ggggccatgg tctatccggt
     1261 agcgtgggcc gcgatcatcc tggttcgact tctcttcgcg aagcaatcga cgactcagag
     1321 agaagcgcag cgctcgggtc gcgtgttgct gctgaagttc ctggcactct ctttcggcat
     1381 ttcgttcttg atcaggatgg gcccgtgggc ggtcccgttc gggctggtgc tggccctcta
     1441 catgctgcca gggctagtgt tgaattgcgt gctcgtgccg ctcggctggc cgcgcgtcgc
     1501 gtactactac gctcgcatcg cgtggccgct cggtttttcg gactccgacg cggtagttgc
     1561 atcgtactac gcggtgcgcg ctcttcactc gaagagtgac gcacaggtat tcgcgaaggt
     1621 ccaaaaggcg ttttccaagg cgaagcccaa ggacatgaac gggggcgcgg tggtcacgct
     1681 cgggctcgtc gcgctggcca acggggagcc agagcaagct cgccagctct ttcatgtggc
     1741 gcacgcgctg aatccgcggt tcatctcgcg cggcacgcgc gcgctcgcga gagactacct
     1801 cgtcgtcgac gcagtgcgct cgggcgccta tcgcaccgcg atccgtctcg gcactacccg
     1861 aaagccgacg ctccggtggg cctacttcgt cgctcgggcg agccagcgcc tgatcgacga
     1921 tcccgccgcg ccgtccaact tcaaactctg gatctactat ctcgtcgcgc cgcgccggcg
     1981 agtgacctac tccctgctcc ggagggcgct cgacagaagc gcacttgccc cttcgatcga
     2041 gcaggcggcg ccagacctcg cgagcgcgct cgctacgctc gccgcaacgc ttcgagactc
     2101 tcccaccacg aacacggcgc gactcaacgc ggtggtctcc agggtcgagg cggcactcac
     2161 cgacccaaag acccacgcgc tcatcgagcg gcgcttgctc ggactcaact cgaacgagag
     2221 cgtcgaggcc gtgctctcgc gatttcgcgc ggagctcgtc gatcgattgg ttccgctcat
     2281 cgagcgcagt gacgaactcg acggcagcgc cgagctcact cccgtcctcg aagaagcgtt
     2341 ccacgaagca cgccgggggg ccttccgtga gctctccgcc ctgtgcaaag actatcgaaa
     2401 gcgaaccgag gaggagaccg gcctcgacac cgacgtcgaa tggaaactct gggcgaactt
     2461 cagagccacc gctgagcgcg tcttgcggat cgacccacaa gcgggacagg aggtcttcgc
     2521 ggaggcgttc attcccatct gcaacttcgc ggtgctccag cacaatacct acaaggcgct
     2581 tgcccttgcg cattcgatgt acgcctggct ccaccgccac gcggactggt caccgaaaga
     2641 ctcactcttg ctcgccaaga acatgaaagc ggccgcgagt tgagcgtcca cggacccgtt
     2701 cgggctgcgc gcaccgacca gttccgtgga cgctgagatc aacgcacgac gaactcggcg
     2761 tcgaaccgca tcggttgcga aaggtgccag cggttcggcg acgtcatttt catctcctcg
     2821 tgcacctcgg ccaactgctc gggactagcc ttggctttga cctcgagtcg gtaccggatg
     2881 agttcggcgc ccggcttcag cgaagcgtcg aggccgaatg cgcctcgcag atcgaagcca
     2941 agcgagctct ggatgcacag gtgttggact tcgatgcctc gcttggtggc ggcagcgacg
     3001 aagccgacca gcatgcaggc attcaatgcg gcaaggagca ggtcctgcgg attgggagca
     3061 gtgttactgc ccaaaatctc gttcggctcg tcgctacgaa tgacgtggcg gcgcgcgatg
     3121 gtccgttcgc cgagttggta gctttcgatc tcggtctggg accgaaggcc gccttgccac
     3181 gtactcttcg cgctgaagtg cacgggcgcg agcgatgggt cagaaaccat cgcggctccc
     3241 gcggccatga aggcggtggt gtcgataccg ttcacgagcg gacctacgct cgttgcaccg
     3301 gcagattcgc tcgcgacaga gtgggttcgt ggatccgtgt tgaacatgaa ttttgcgtct
     3361 ccttgggtgg tacttggtgt cttcaatttg gctgttctcg actgaaactc tcggccgcgc
     3421 gtgcgacgcg ctcgagcgcg tccggggcca cgccgctcaa tgcgatcaga acctccgggt
     3481 gggtgatgcc tgccaacaag aagcagccgc gttgcgaggc ctgttgaaca cgcaagaact
     3541 cggctcgttg ctccgggctc aggcggcggg cgctcgcgag ctcgaacgag accaagtcga
     3601 gctcgacttg tacccgaagc gcgtcatcca tcgacctcaa gatgtccagg tagtcggaga
     3661 cgccttcgct tcgcgcgctc gcggtagcag aactcactag ctccgcgacg agctgcgcat
     3721 cgaggcgcgc gtgttgcgct tcctccatcc agtgggcgcg cagtagcttc ttgaacgatg
     3781 cctccagtgt ttcgtcggcg cgcacgcaat cgctccagtg tcgttgagtc atccactcga
     3841 tgtgcaagat ggcgagcgca acaccgagct ccgagtgcgc gagcactgcg ctcgaaaagt
     3901 ccgacgcagg cccgatcaac gcgggccgcg ccccgaaccc gcgctcgaac acgcgcaaga
     3961 agcgtcgaaa tagggcaatg tgcttcgtct cttcccccgc aaagccaacg agcgcccgca
     4021 gcgcgtcgag gtcggcgcct ctgggcttcg cctgcgcgag aacgaacggc acgatgaact
     4081 cttcgacgat cccgaacagg ccgaggtagc cgtgcgccct gatgtggccc agcagaagcc
     4141 gctcacgggt attcaggaaa tcgagtgaat cgaccgcggt gagagcgttg ggcagaaacg
     4201 gccgcgcgaa gtcgaagctt gcgtcttcgg cgaggacgtc gtcgaggcgg aacgccgcgc
     4261 gctcggacaa tgcgagcgtg cgcgcccaaa ccggcgcgtc agatcgtgac accgggtgct
     4321 ccagcgccgg agagagagag ttcggggagt ccccgtgttc ggcggctggg cgcgtcgagc
     4381 accgactgga aacgcacttc gagctgctcg acgagcccgg acccgtcgag gcccgcgagc
     4441 accgggcctt cggcgtagct gcgtgcactc gcgaggtctc gaaagaggta gagcccgccc
     4501 gcggtgtggc cgtccgcgct caatagccag agtttccaaa ttagcccggg cacggtcgcc
     4561 gtacgcggcg cgatccgctc gcaatgcgaa agccattctt cggaactgac tttcaaagcg
     4621 aacgttactt gcaaacaaac atggttcatg gtgagtacct ccgtgtgtcg aggtaagatg
     4681 cggctctcga cgctcgccgg catgagacgg gggcgagaag aagatgagaa gaacgtgaga
     4741 aacccgaaga cccgcctttc ggagcgcctg gctcgctcga accgcgaggc gctgttcggt
     4801 cgggaacggg agctcgcgct gctcgaggct cagctcgcac cagagggggc agtggtcacg
     4861 ttcgtgcacg gcgcgggcgg catcggaaag acgtcgctgc tcgccgcggc catgccgcgg
     4921 ctcgaggagc tcggcgcgct cgtcgtgacg ctcgacgcac gctcgattgc tccgactgcg
     4981 cgagcgttct tggcggcggt acgtgccaag gtgggcgtgg aaccgttcgg agttcacgac
     5041 ctccaagaag agattcgaga cgcggaggac attcgcgcgc tcgcgcaggt actcgagcgt
     5101 gaaccagcgc cacatgcgtt cttggtcgac gaattcgaca acctgcgtct gctggatgac
     5161 tggttgcggc gcgagttctt gccggagctg ccagagcgaa cacgctgggt gtttgcgggg
     5221 cgcttcggtc ctcgcgcggc ctggttgacg actccgggtt ggtcacgcgc ggtggtctcg
     5281 ctcaagctcg agccgctcga cgaggctgcc gcgcgcacct tgctcttggc gcgtggggtc
     5341 cccgaagagg aagttgccag gctgactcgg ctcgcgcgcg gcacaccgct ggcgctgacc
     5401 ctcgtgacgg cgaagagcga ggccgagccg accgtgaacg aaaacgctgt gctcgcggcg
     5461 ctcgccgagc gatccgttca ggcgctgcct ctggcgttac gcgaggcgct agaggcggcg
     5521 agcgtcgttc gaagggtcac ccgcgacatg ctcgtcgagc tgctcggagc ggcctacgac
     5581 gaatctttgc tcgacgagct cagtcagctc tcgttcgtcg aacacgccga agatggcctg
     5641 gtgcttcacg agaccgtccg tcacgccgtc gcgtcgaggt tgcgggcgct cgatcctagc
     5701 cgccatcagc gcttgcgtca gtcggcgtcg gcggtgatcg cacgcgctct cgacagccag
     5761 cccaagcccc gctcgggcgc ttggcgcctg ttggcggaca tgttgtttct gtcggacgaa
     5821 cctgggcttc gcgaggcctt cttccccacg cgcgacaatg gcgccgcggt aatggacgcg
     5881 gcgctcgcga gtgaccacgc cccgcttcgc gcactcgtcg aacgcaacga cgaaggcccg
     5941 tggctcgagg tcttcgaagc ctggtggcag cacgcgccaa agtgctttcg cgtcgtacga
     6001 ggcagtcatg gggcgttgct cggcttcagc ctcgtggttc cctccggcga cattccaaga
     6061 gcactcacga gcgaagaccc gctgcttgga acatggcaag ccgatctcgc ggcttcgcca
     6121 ggcggtgagc gcggcgcgct gttcatgcga cgggcactcg cggcgcaagg tgagacggaa
     6181 gaagccacgc gaagcctgat ctggctcgac gtaaagcgct cctacgtcga gcgacccgag
     6241 cagtgggcgc tgtacgtcgc tactcgcgaa gcggaaagaa gagctctccg tccttgcgcg
     6301 tctcgggttt cgacgcgtgg cgctcgagca cgcgggtgaa gcgacgattc agctacgctt
     6361 tggcgaaaag ggcgtttggg ggtggctccg agatctcatc caggggagca accgagatct
     6421 cttccaaggg gacaaccgag atctcgtcca ggggagcaac tcgcccgaac tgaggagcgt
     6481 cgaaccaggc gatccgagag gaacctcggt tccgtcatgg cacttggacg aagcgcagcg
     6541 cgccttggta atcgtcggcg agcgtcagcc gctgagtgcg ctcgagtatc gagcgctatc
     6601 gtacctgctc gagcaggctg gacgagtggt cacgcgtgag caactgctcg cggccgcttg
     6661 ggagcagagc tactcgggta gcaacgtcgt cgacgcgctg attcgcttgt tacggaagaa
     6721 gctcgggccc tttgctggag atctcgaaac cgtgcgcggt catggttacc ggctgtcgcg
     6781 ctccggtcgc cgcgcagacg tcacgtaaag cgattgcaaa atatcgagcc cctcgacgtc
     6841 cgagccaaat cgccgttacg gctcggcccg cgagcgccgc g
//
LOCUS       DASTKS010000413         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_65981,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000413 DASTKS010000000
VERSION     DASTKS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65981"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1623)
                     /gene="dinB"
                     /locus_tag="VFQ35_08655"
     CDS             complement(373..1623)
                     /gene="dinB"
                     /locus_tag="VFQ35_08655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET9930743.1"
                     /translation="MDFTRWVLHADMDAFYASIEQRDRPELRNKPVIVGAASARGVVA
                     AASYEARKFGVRSAMPGFRAKQLCPDGIFLAGNMPHYAAVSAEIHEVFTSFTPQVEPI
                     ALDEAFLDISGSLGLFGQPKQLAERLKQAVFEKTALNVSVGIAPNKLVAKIACTFGKP
                     NGLTVVEPHAVIEFLRPLPIRRLWGVGPVLAERLQSLGIRTFADLSDFDPRKLDQAIG
                     RRALELQALARGEDHRPVEADRIPKSYGEESTFERDVSARDVVTAALTAHAEAVARRV
                     RRDSFRGRTVTLKIKLGIAKGTRIARGANPFGEEEPLYPLLTRSRTLPEPTDSGSTIR
                     EVAVSLWDEAQVQTPVRLLGVSLSNLVAREGEQLDLFARPRKDRLGPTLDAIAERFGP
                     NAISRAVDAPGKVTPSGRKKRGEP"
     gene            1904..2398
                     /locus_tag="VFQ35_08660"
     CDS             1904..2398
                     /locus_tag="VFQ35_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930744.1"
                     /translation="MRLTSILGVVALGCSLAVGCGGDDDDSTPGKGGGAGTAGKGGNA
                     GASSGGKGGASSGGSSSGGKSSGGSTSGGSGNGGSMTGGAAGEGGTPEGGMPGTGGKA
                     SGGTAGKGSGGTGTGGTGSGGKGGGGGTGGITVTGGAGGAGGDGGVGPLGGEAGMAGA
                     AGHH"
BASE COUNT          489 a          848 c          906 g          489 t
ORIGIN      
        1 cagcaagggg cgcgccgccg attgattcta ttttcttcac ttgccgcttt gagcctcgac
       61 gcggagccca atagcgtcgc tctcgcactg ctccctgttc ggcgagagct ccatgctgcg
      121 cgccccatag aaagcgggca aaaactggat gcaccaaggg ggccttcaca gcatcggtgg
      181 aaaaccgacc gctcgagaat cacgtccatg caaactgaag cggcgccgtc tggtctcccg
      241 gcgcggcagg atctccgttg cggagacatg cgagcgagta ttcggggcaa gcgccctcgg
      301 atcgccgtgt cacggtccct ccgtagattg cgccaggacc tcaataccgc acgagtgtag
      361 cgggcgacga agtcatggct cgccccgctt tttgcgaccg ctcggcgtga ccttgccggg
      421 agcgtcgacg gctctcgaga tggcgttcgg cccgaagcgc tccgcgattg cgtcgagagt
      481 cgggccgagc cgatctttgc ggggacgagc gaacaaatcg agttgctctc cttcgcgcgc
      541 gacgaggttc gagagcgaca ctccaagcag ccgcaccggc gtctggactt gcgcctcgtc
      601 ccagagcgac accgccacct cccgaatcgt gcttccgctg tcggtcggct cggggagggt
      661 gcggctccgc gtgagtaacg ggtagagcgg ttcctcttcg ccgaatgggt tcgctccccg
      721 cgcgatccgc gtgcccttcg cgataccgag cttgattttc aatgtcacgg tgcgaccgcg
      781 aaatgagtcg cgccgcacgc gtcgcgcgac cgcttctgcg tgtgccgtga gcgcagccgt
      841 caccacgtcg cgtgcggaga cgtcgcgctc gaaggtgctt tcttctccgt aagacttcgg
      901 gatccggtcg gcttcgacgg gtcgatgatc ttccccccgc gccagcgcct gaagctctaa
      961 tgcccgccgg ccaatcgctt gatccagctt acgtgggtcg aaatcgctga ggtccgcgaa
     1021 cgtccgaatc ccaagcgatt gcagtcgctc cgcgagcacc ggcccgacgc cccagagccg
     1081 tcgaatcggc agcggccgaa ggaactcgat taccgcgtgt ggctcgacga cggtcaatcc
     1141 gtttggcttt ccgaacgtgc atgcgatttt tgcgacgagc ttgttcggag caattcccac
     1201 ggaaacgttc agagccgttt tctcgaacac ggcttgcttc agtcgttccg cgagttgctt
     1261 cggctgtccg aacaacccga gcgagcccga tatatcgagg aacgcctcgt ccagcgcgat
     1321 gggttcaacc tgaggggtga agctcgtgaa aacctcgtga atttccgccg aaaccgcggc
     1381 gtagtggggc atgttccccg cgaggaaaat gccgtccgga cacaactgct tcgctcgaaa
     1441 tccgggcatc gcggatcgaa caccgaactt gcgggcctca taagatgcag ccgccacgac
     1501 tccgcgcgcc gaagcggctc ccacaatgac gggcttgttt cgaagctcgg gtcgatcgcg
     1561 ctgctcgatg gatgcgtaaa acgcgtccat gtcggcgtgt aaaacccagc gtgtgaaatc
     1621 cacggtgact cgaacttctg tgcgcccggc agcggcgcgg cgctcagtct gacatttcta
     1681 ggaggtgtcc ccagtgtcag cgaatcgctc gcagcaggga ggacgctttg gacacccctc
     1741 ggccactacg atattgcgag catgccgagt cgcagcccga cggttcgatt tgtcaatttg
     1801 agggttgcga aatggtggtt cccgctacta gatcgctcag gcgaaaccac gaccagagcc
     1861 tcgatggggt ttggtcgacg gcgggagacg gaagctaatg aaaatgcgtt tgacttcaat
     1921 tttgggtgtt gtggcgctcg gctgctcgct cgctgtcggt tgcggcggtg acgacgacga
     1981 ttcgacgcct ggcaagggcg gcggcgctgg tactgccggc aaaggcggca acgcaggcgc
     2041 tagctctggc ggcaaaggtg gcgcgtcgag cggtgggtcg agcagcggcg gcaagagcag
     2101 cggcggctcc acgagcggtg gctccggcaa cggtggttcc atgactggcg gcgccgctgg
     2161 cgaaggcggc actcccgaag gtggaatgcc tggcaccggt ggcaaggcca gcggtggtac
     2221 cgccggtaaa ggcagcggcg gcaccggcac tggtggcacc ggcagcggcg gcaaaggcgg
     2281 cggcggtggc accggcggca tcaccgtgac cggtggtgct ggcggagctg gcggcgacgg
     2341 tggtgtcggc ccgcttggcg gcgaagccgg catggctggc gcggccggcc atcactgagc
     2401 cagagcgctc aataagctga gcgggatcgt gtaacgcttc gcctccggcg gagtgaaggc
     2461 gcgatcccgc catgctcgct tagagcccgc gaatggataa cccagttgcg agcgagccga
     2521 gcccgcagca agaggatcgg ctctcgcagt atgccttagc cattcgaggg ccctttagcg
     2581 agccaacgaa caaggacgct atcccgcgct cattcgtcga gctcgtaacg cgaaggtgcg
     2641 atcccgcccg cgcttgctgg gcgagctcgc gaagcgagaa ggaagcgatc ccgcgctcat
     2701 tgggcgagct tgcaacgcga aggtgcgagc tc
//
LOCUS       DASTKS010000414         7921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1404934, whole genome shotgun sequence.
ACCESSION   DASTKS010000414 DASTKS010000000
VERSION     DASTKS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404934"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFQ35_08665"
     CDS             <1..615
                     /locus_tag="VFQ35_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9930745.1"
                     /translation="RRRIASNLHDGAGQTLALAQMRLARARLGVGPPWRADLEACERL
                     LEDADAQIRRVTFDLSPPVLYDLGLKPALEWLAEQMNELYGLSVEVGGDRDLDLEPAT
                     AAVAYRAVRELLTNVAKHGRVRDAKVKLRQEGASVKIVVEDHGIGIDTETSKRTDSFG
                     LFSINEQVVRLHGTLDVQSTPTRGTTVTIVIPSGHPSGQRSRSS"
     gene            612..1250
                     /locus_tag="VFQ35_08670"
     CDS             612..1250
                     /locus_tag="VFQ35_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9930746.1"
                     /translation="MKILLADDHAIMREGLRVILERMPSVEVVGETDNGRDAVRMARE
                     LRPDILLMDISMPGLNGVDATRLITGTLPNTRVIGLSMHADRRYVLSMLTSGASGYLL
                     KRAASSELALAIEAVAAGHKYVSPAIADAVIEALPKAATQSAGPDISPREREVLQLLA
                     EGRTSKEIAAILDVAVTTVETHRRQLMERLHIHSVAELTKYAVREGLTSLND"
     gene            complement(1289..1942)
                     /locus_tag="VFQ35_08675"
     CDS             complement(1289..1942)
                     /locus_tag="VFQ35_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9930747.1"
                     /translation="MRVFLADDHRLLREGLRALLEREGMHVVGEASSGRELLEELERT
                     RPDLVVVDISMPELNGIDATRRVLAIAPETSVIALSMNGDRRQVLGMLEVGASAYVLK
                     SSAGRELLHAIAEVSAGRKYVSPAIADVLPELPEGKRDSSPMRSSELPATALSARERE
                     VLQLVAEGNSSKEIAGKLNIAVGTVETHRRQIMERLGLRTIAALTKYAVREGLTTLD"
     gene            2870..6214
                     /locus_tag="VFQ35_08680"
     CDS             2870..6214
                     /locus_tag="VFQ35_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9930748.1"
                     /translation="MLESRQPMCVAWGADWRLLYNDAYRTLLGDKHPRSLGRPAREVW
                     AEVWSQIVHDFEAVRRGESTYFENRPFVILGNGYSEERYFTISCTPVRLVDGEVGGAF
                     ISAVETTPGKRAEQRAELSARLADKLHALLEPHEIALAGAQVITEALRVARARWLRVD
                     EDAASLLGEHPAPRAASSSPRSASPRELDTALARGEPVAVAEVQSDSRVTSDERALLV
                     SDGIEALCRVPALKNGVCVGILEVHSRTPRRWLAADLSLLEDAAARIQVALDWAEAET
                     ARREVEARNAALFERSPVAMVVARPDGRRVTMNDAFRRLFELAADEPAGLTALDLGLI
                     SPTTRQELRSALARQGEVHDFPCTLRTKTQRELEARIDVLPVTVKKEPAVLTVVRDRT
                     ELTRAERTADLYRAAKEKLADVEAFDRLHRVSSRYFGAEGPHTVLEEILDAAIAISGA
                     DFGNIQAVDRDCKSLRLLAHRGLPEAWLAYWSAHPDADSCALACARGERVVIEDVLSN
                     RAFSAPEVAQLQLESSIRAVISTPLRTRAGATVGVVSTHFRSPGRPSERALRHLDLLA
                     QQAAEIIARVNMERALRRAEARSRGILALLSDAIISIDERRRITEWNRAAELMFGYTQ
                     QEIIGASLDVLVPERHRPGHAEHILAFAAEPRTTRRMDHEHTVGLRRSGEEFPLEGML
                     SKLEADGERIFTVSIRDRTEDRRREQERTVLAEIGSALASLDLERALHDIAVALAKTL
                     SDYAFVFLPAEDGALRCAAAASRDADEVWAADSMLRLRPSAETQHPAWRALKEGATVV
                     DWVLPESHATGAASTDHDAAIADAASRCTTCVPLLVAGRAVGVLGFGSTARPLDEREL
                     RLAEEVARRCALFVENARLLEAEKRATCLRDEVLATVAHDLGNWVGTIALQLQLLRRS
                     GPEPERRSTRPLDAIEQATEIMHRIIQDLMHVARIEAGTFEVTRSWLSHPEHLLDQIV
                     EALRPAAAKRSLELRMDVSGPLPAISADRDRLVQVIENLASNALKFTAKGTVTVGARA
                     ADGEVVFWVADTGVGIPSEELGDIFDRFWRATGTRPAGNGLGLAIVKGIVDAHGGRLW
                     VESELGHGATFYFTVPIAEPRSDARE"
     gene            complement(6300..7688)
                     /locus_tag="VFQ35_08685"
     CDS             complement(6300..7688)
                     /locus_tag="VFQ35_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9930749.1"
                     /translation="MTQARILVVDDEASARALLTAALSDAGYEVLAVADGFKALNEIE
                     NFEPELTITDLLLPGMNGVELVRRLRARKASAAVVVATASSEPSSIIAAMRAGARDYL
                     CKPLDLEELLSVVERAVEQARIANEVATLRAGAERRSLPPAFVGSCPEIVQLFKAIAQ
                     VAPSRATVLVTGESGTGKELVARAIHERSPRANRPLVPLHCAALSEGVLESELFGHER
                     GAFTGAAQRRKGRLEEADGGTLFLDEIGEISPSVQVKLLRVLQERAFERVGSSLTHHA
                     DLRFIAATNRNLRELVAQGKFRQDLWYRLNVVNIHLPPLRERRGDIVALATHFLDEQC
                     ALTERGPMRFSDAALARMLSYSWPGNVRELKNVVEHAVVFADGDVIEEGHLPVDTGGR
                     PTHRVPPIPGSSLSELERYAILRTVEATAGSRAKAAAVLGVSVRKLQYRLQQYSASPR
                     GIKAPLKPFHDA"
     gene            complement(7741..>7921)
                     /locus_tag="VFQ35_08690"
     CDS             complement(7741..>7921)
                     /locus_tag="VFQ35_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930750.1"
                     /translation="SAPRASRDSGFVERRGRRMLDASGGSIWIDEGPVARTVCLRLPN
                     HRAGVEEDALEQNAQ"
BASE COUNT         1416 a         2623 c         2537 g         1345 t
ORIGIN      
        1 cggcgccgca tcgcgagcaa cctgcacgac ggtgctggcc agaccctcgc gctcgcacag
       61 atgcgcctgg cgcgagcgcg gctcggcgtc gggccgccgt ggcgagcaga tctcgaggcg
      121 tgcgaacggc tcctcgagga cgccgacgcg cagatccgcc gcgtcacgtt cgatttgagc
      181 cctcccgtgc tctacgattt ggggctgaaa ccagcgctcg agtggcttgc agagcagatg
      241 aacgagctct acgggctcag cgtcgaagtc ggcggggacc gagacctcga cctcgagccc
      301 gcgaccgcgg cagtggcgta ccgcgcagtt cgggagctgc tcaccaacgt cgcaaagcac
      361 ggccgcgtgc gagacgcgaa ggtcaagctc cgccaggaag gcgccagcgt gaagatcgta
      421 gtcgaggacc acggcatcgg catcgacacc gagacgtcga aaaggaccga ttcattcggg
      481 ctattcagca tcaacgagca agtcgttcgg ctgcacggca cgctggatgt gcagtcgacc
      541 cccacgcggg gaaccacggt caccatcgtc atcccgagcg ggcatccatc gggacaacgg
      601 agtcgttctt catgaagatt ttgctggccg acgatcatgc gatcatgcgc gaagggctcc
      661 gcgtgatcct ggagaggatg ccttcggtcg aggtcgtcgg ggagaccgac aacggtcgcg
      721 atgcagtccg gatggcgcgt gagttgcgcc ccgacatact cctcatggac atctcgatgc
      781 cgggactcaa cggtgtagac gcgacgcgtt tgatcacagg cactttgccc aacacccgag
      841 tcatcgggct cagcatgcac gccgatcgcc gctatgtgct gtcgatgttg acgtctggag
      901 cttcgggcta cttgctcaag cgggcggctt cgagcgagct cgccctggcg atcgaagcgg
      961 tggccgccgg gcataaatac gtaagcccgg caatcgcaga cgcggtcatc gaagcgctcc
     1021 cgaaggcggc cacccaaagc gcaggccccg acatttctcc gcgagagcgc gaggtgctgc
     1081 agctcctcgc cgagggccgg acatcgaagg agatcgcggc gatcttggac gtcgccgtga
     1141 cgacggtcga aacccaccgc cgacagctga tggaacggct tcacatccac tccgtggccg
     1201 agctcaccaa atacgctgtc cgagaagggc tgacgtcgct caacgactga cgactgatcg
     1261 ccaatcgtca aaatctagcc cgccgagttc aatcgagggt ggttagccct tcacgcaccg
     1321 catacttggt gagcgcggcg atggtgcgca ggccgagtcg ctccatgatt tggcggcggt
     1381 gcgtctcgac cgtaccgact gcaatattca atttccctgc aatttccttc gaagagttcc
     1441 cctcagcgac gagctgaagg acttcgcgct cacgcgccga aagagccgtc gccgggagct
     1501 cggacgaccg catcgggctc gagtcacgct tgccttccgg cagttcgggc agcacgtcgg
     1561 cgatggcggg actcacgtat ttgcgaccgg cgctcacttc tgcgatcgcg tgcaacagct
     1621 ctcgccccgc gctgcttttc agcacgtagg cggatgcccc gacctcgagc atcccgagca
     1681 cctggcgccg atcgccgttc atcgacaacg cgatgaccga ggtctccggc gcgatggcga
     1741 gcacgcgccg agtcgcgtcg atcccattca gttcgggcat cgatatgtcc acgacgacca
     1801 ggtcgggccg cgtgcgctcg agttcctcca aaagctcgcg cccagaggac gcttcgccga
     1861 cgacgtgcat accctcgcgt tcgagcagtg cccgaagccc ctcgcggagc aagcgatgat
     1921 catcggcgag aaagaccctc attcgtcggg gtgacaccag gagttgcgcg gcgccaatcg
     1981 gaacgtacct cgtggcggcg acctgccgca agcagagccg tcgcgccccg tttccccggc
     2041 gtgacgagcg aaacagcgag accgaaaaat gcgacccgac cgattcacgg ttccgcgcct
     2101 tcgctgattc gagggcgtcg caaagttctt cgccctgctc cgagccaaga tacggctgcg
     2161 aaaggcgcga cgtcgctctg ttcttcgcaa agcttcgccc cttcgcgggg ccgcgcttcg
     2221 cgctcgagcg tcccccatct cgcgattggc agaaacgtgt atgccgctca acagagcgtt
     2281 gagaaacgcc tcgcgtcacg agagaccaat gccagacagc cacgatgcgc agccgcaccc
     2341 tccgcacgcg acaggcggcg cgcgtttcta caacgccctg gtagggcgaa acaccggtga
     2401 agctagattg cgttcaccac tcatgtcgga aaccgcaagg cgcgcggtgg aggagcagtc
     2461 gctcggcgac ccctcatcga ttggaatccg cattagctca cgagcaatcg gggttgcggg
     2521 atgactcgcg tccacgtatg caggccatgg tctcgccgct cgaccgtggc tcacttgcga
     2581 atggcaaaat cttcggctaa acgcctatcg agaaacgact cgtacggatc cgtcactcgg
     2641 cattcgagtg agttcgaata gatgccaggc catcttgggc atgggagtga acggccgatt
     2701 ctgcccctcg gcattcaagc acgtgactaa gctacttagc agggaacaca cggagtggct
     2761 ggctgggagc ggtgagctgc gggagctcat tcgctcgaca gactgggctc agacgtcgct
     2821 cgggtccgcc gaccattggc cggcttctct tcggaccaca ctgagtctca tgctcgagag
     2881 ccgtcagccg atgtgcgtcg cgtggggcgc cgattggcgg ctcctttaca acgatgccta
     2941 tcggacactc ttgggtgaca aacacccgcg ctcgctcggt cgacccgcac gcgaagtctg
     3001 ggcggaagtg tggtctcaaa tcgttcatga cttcgaggcg gtccgccgcg gagagtcgac
     3061 ttatttcgag aatcgcccgt tcgtcatcct cgggaatggc tacagcgagg agcgctattt
     3121 tacaatcagc tgcacccccg tgcggctcgt cgacggcgaa gtcggtggag ccttcatttc
     3181 agcggtggag acgacgccgg gcaaacgcgc ggagcaacgc gcggagttgt cggcgcgact
     3241 tgcagataaa ttgcacgcat tgctcgagcc gcacgagatc gcgctcgccg gagcgcaggt
     3301 catcacggag gcgcttcgcg ttgcgcgggc gcgctggctc cgagtcgacg aggacgccgc
     3361 gtcgctcctt ggcgagcatc cggcgccgcg agcagcgtct agctctcccc gcagcgcgtc
     3421 tccgcgtgag ctcgacaccg ccttggcgcg cggcgagccc gtcgcagtgg cagaagtcca
     3481 gagcgactcg cgggtgacgt ccgacgagcg ggcgttgcta gtgagcgacg gtatcgaagc
     3541 cctatgtcgt gtgcccgcgt tgaagaacgg cgtttgcgtc gggatactcg aagttcactc
     3601 ccggacgccg cgccgatggt tagcggcgga cctctcgctc ctagaggacg cagccgcgag
     3661 gatccaagtg gctctcgact gggccgaggc tgaaaccgcg cggcgtgagg tcgaggcgcg
     3721 caacgcggcc ctgttcgagc gatctccggt tgcgatggtc gtagcgcgtc ccgacggccg
     3781 gcgcgtgacg atgaacgacg cattcaggcg cttgttcgag ctcgccgcgg acgaaccggc
     3841 ggggctgacc gcgctcgatc tcgggctcat ttcgccgacg acgcgacagg aactccgcag
     3901 tgctctcgcc cgccagggcg aagtccacga cttcccgtgt acgctccgca ccaagacgca
     3961 aagggaactc gaggcgcgaa tcgacgtcct gcccgttacc gtcaaaaagg agcctgccgt
     4021 cctcaccgtc gtgcgagacc gcactgaatt gactcgagcc gagcggaccg cggatctcta
     4081 ccgagccgcc aaggaaaagc tcgctgacgt cgaggcgttc gatcgcctgc accgcgtgag
     4141 ctctcgctac ttcggcgcgg aaggaccgca caccgtgctc gaagaaatcc tcgatgccgc
     4201 catcgcgata tcgggcgcag acttcggcaa catccaggcc gtcgatcgcg actgcaaaag
     4261 ccttcggctc ttggctcacc gcgggctgcc cgaggcttgg ctcgcttact ggagcgccca
     4321 tcccgacgca gactcctgcg cgctcgcttg cgcgcgcggt gagcgggtcg tcatcgaaga
     4381 cgtcctctcg aaccgcgcat tttcagcgcc agaagtcgct cagttgcaac tcgagtcgtc
     4441 gattcgcgcc gtcatatcga ctccccttcg cacccgcgcc ggggcgaccg tcggtgtcgt
     4501 ctcgacgcac ttccgctcgc cagggcggcc gagcgaaaga gcgttgcgtc acctggattt
     4561 gctcgcccaa caggcggcgg agatcatcgc gcgggtgaac atggagcgcg cgctacgcag
     4621 agccgaagcg aggtcccgcg ggatcttggc gttgctttcg gatgcgatca tttcgatcga
     4681 cgagcgccgg cgcatcacgg agtggaaccg cgccgccgaa ctcatgttcg gctacacaca
     4741 gcaagaaatc attggcgctt ccctcgatgt gctcgtcccc gagcggcatc ggcccggtca
     4801 cgccgagcac attctcgcct tcgcggccga gccgcgcacc acccggcgca tggatcacga
     4861 gcataccgtc ggccttcgca ggagcggcga agagtttcct ctcgagggga tgctctcgaa
     4921 gctcgaggca gacggcgaac gcatcttcac cgtctcgatc cgcgatagaa cggaagaccg
     4981 ccggcgcgag caggagcgga cggtgctcgc cgagatcggc tccgcgctcg cctcactcga
     5041 tctcgagcga gcactgcacg atatcgcggt cgcgctggcc aagacactct cggactatgc
     5101 tttcgttttc ctgcccgccg aggacggagc gcttcgttgt gctgcggcgg cgagccgcga
     5161 tgccgatgaa gtgtgggccg cagactccat gctgcggctg cggccttccg cggaaacgca
     5221 gcacccggcg tggcgagcac tcaaagaagg cgccaccgtc gtggattggg ttctgccgga
     5281 atcgcacgcg acgggggccg ccagcaccga ccacgatgcc gccatcgccg acgcggcctc
     5341 acgctgcacg acgtgtgttc cgctgttggt cgccggtagg gccgttggtg tcctcggctt
     5401 cggctcgacc gcgcgacccc tcgacgaacg agagctccgc ttggcggagg aggtcgcccg
     5461 acgctgcgcg ctcttcgtcg aaaacgcacg cttgctcgag gcggaaaagc gcgccacctg
     5521 cttgcgcgac gaagtgctcg cgacggtcgc gcacgatctc ggcaattggg tcggcacgat
     5581 tgctttgcag ctccagctct tgcgtcgctc cgggcccgaa cccgagcgcc gcagcacgcg
     5641 accgctcgac gcgatcgagc aagcaaccga gatcatgcac cgcatcattc aagatctcat
     5701 gcacgtcgcg cgcatcgagg ccggcacctt cgaggtcacg cggtcatggc tctcgcatcc
     5761 cgagcacctg ctcgaccaaa tcgttgaagc tctccgcccc gcggctgcga agcgctccct
     5821 cgagctccgg atggacgttt ccggtcctct gccggcaatt tcggcggacc gagaccgtct
     5881 cgtgcaagtc atcgaaaacc tcgcgagcaa tgccctcaag tttacggcaa agggaaccgt
     5941 cacggtcggc gctcgtgccg ccgacggcga ggtcgtgttt tgggtcgcgg acaccggcgt
     6001 cggaatcccg agcgaagagc tcggcgacat cttcgaccga ttttggcgcg ctaccggcac
     6061 acgcccagcg ggcaatgggc tcgggctcgc gatcgtgaaa ggcatcgtcg acgcccacgg
     6121 cgggcgtctc tgggtcgaga gcgagctcgg ccacggggcg acgttctatt tcacggttcc
     6181 tatcgctgaa ccgcggtcgg acgcccgcga atgaatatgt cagtggcgag caagccgaga
     6241 ccgcagcgga gcgggcgcgt gaggagtcag cagcggacgc cttttagcac tgacacgctt
     6301 caagcgtcgt gaaatggctt cagcggcgct ttgatgccgc gcggcgatgc ggagtactgc
     6361 tggagccggt attggagctt acgcaccgac acgccgagca cggccgccgc cttcgcgcgc
     6421 gagccagcag tcgcttccac cgttcgcaat atcgcgtagc gctcgagctc gctgagcgac
     6481 gaacccggaa tcggcgggac gcggtgcgtg ggtcgaccgc ccgtgtccac cgggaggtgc
     6541 ccttcttcga tcacgtcacc gtccgcgaag acgaccgcgt gctcgacgac gttcttgagc
     6601 tcccgcacgt tcccgggcca cgagtacgag agcattcggg cgagcgcggc gtcgctgaag
     6661 cgcatcggtc cgcgttcggt gagcgcgcat tgctcgtcca ggaagtgcgt cgcgagagca
     6721 acgatgtcac cgcgtcgttc gcggagcggc ggaaggtgaa tgttgacgac gttcagccgg
     6781 taccacaaat cttgccggaa cttcccttgc gcaacgagct cgcgaagatt tcggttcgtc
     6841 gcggcgatga agcgcaaatc ggcatggtga gtaaggctgc tgccgacgcg ctcgaaggcg
     6901 cgctcttgca agacccgcag cagcttgacc tgcacgctcg gcgagatctc cccaatctcg
     6961 tcgaggaaca gcgttcctcc gtcggcctcc tcgagccgac ccttgcgtcg ctgggccgcg
     7021 cccgtgaagg cccctcgttc gtgaccgaag agctcacttt cgagcacgcc ttccgaaagt
     7081 gccgcgcagt ggagcgggac gagcggcctg tttgcgcgcg gggaccgctc atggatcgcg
     7141 cgggcgacga gctccttgcc ggtaccggat tccccagtaa cgagcacggt cgcacggctc
     7201 ggcgcgactt gcgcgatcgc cttgaagagc tggacgatct ccgggcagct tccgacgaac
     7261 gcgggcggaa gcgaacgcct ctcggcgcct gctcgcagcg tcgcaacctc gttcgcgatg
     7321 cgcgcctgct cgacggctcg ttccacgacc gacagcagct cttccaggtc cagcggctta
     7381 cataggtagt cccgcgcgcc ggcgcgcatg gccgctatta tcgacgacgg ctcggaagat
     7441 gcggtcgcga cgacgaccgc cgcggaggct tttcgggctc gcaaacgacg aacgagctcg
     7501 acgccgttca ttccggggag cagcagatcc gtaatcgtga gctccggctc gaaattttcg
     7561 atttcattga gcgctttgaa gccgtcggcg actgcgagga cctcgtagcc agcgtcgctc
     7621 agcgccgccg tcaacagcgc gcgcgccgac gcctcgtcgt cgacaaccag aattcgtgcc
     7681 tgcgtcatgg gatctcgatg gggctacgag ggtcgtgaac ttggactccg aaccgcgcgc
     7741 tcactgcgcg ttctgctcga gggcatcttc ttcgactccg gcccggtggt tgggcaatcg
     7801 caagcacacc gtgcgcgcga caggcccttc gtcgatccaa atggacccgc cactcgcatc
     7861 gagcattctg cgaccgcgcc tctccacgaa gccagagtcg cgggacgcgc ggggcgccga
     7921 c
//
LOCUS       DASTKS010000415         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1181036, whole genome shotgun sequence.
ACCESSION   DASTKS010000415 DASTKS010000000
VERSION     DASTKS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181036"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFQ35_08695"
     CDS             <1..454
                     /locus_tag="VFQ35_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930751.1"
                     /translation="IVPLDLSLKSTVARGEGYRLAAFGSVSGAAGIENGPAWLGRVGL
                     VGQACFDVHCRSSATLGGTAVLLGPATFIATGAGAVLRVSELFAILVEVDSLVPAGRN
                     LADYHGIIGGVGFRWSGRRWAVDGALYSSLNGEGPVVPFLAASYRFLR"
     gene            518..1504
                     /locus_tag="VFQ35_08700"
     CDS             518..1504
                     /locus_tag="VFQ35_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9930752.1"
                     /translation="MKESLSLGVDLGGTKTEAVVLRRVAAPHPDDAHFDVLARERVPT
                     PSSAGYDAVLAATAELVRQVLAQAGLSARETSIGVGMPGAVTRREGLVKNSNSVCLNG
                     RPFREDLSRALGQTVAFENDANCFALAETELGAGRAHRDGIVFGVIMGTGVGGGLVVH
                     GRVWPGLQSLGGEWGHHAVGPWRRAGAAEAPGEFGLGLPALPVCYCGKTGCLELYAAG
                     PAVERDYANRSGERRSLREIAERHREDAHAAASIDQLLEAFGRGLANVIDIVDPSAIV
                     LGGGVSNLSCLYDEGVDRVRRYVFNDELLTPILKHELGDSAGVLGAALLNGD"
     gene            complement(1505..1975)
                     /locus_tag="VFQ35_08705"
     CDS             complement(1505..1975)
                     /locus_tag="VFQ35_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HET9930753.1"
                     /translation="MMGMPEPSEHHRKLHYLAGTWIGEEHLSPSEWGPGGIAIGRYIG
                     RVDVDGFFVIQDYVEEKDGRVVYRGHGVFGWDEQKKTYTWYWVDSLGMAPSAPSRGRW
                     EGNTLVFEHDTTAPQRGRYTYRFNDEDSYLFTIENTQDGGQTWQQFLEAEYHRQ"
     gene            complement(2022..2492)
                     /locus_tag="VFQ35_08710"
     CDS             complement(2022..2492)
                     /locus_tag="VFQ35_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9930754.1"
                     /translation="MKIAEIMTRDVHSCTPTDTLATAAQIMWENDCGAVPVVDRDGRL
                     VGLITDRDLAMAAQLQGVALSDSSVSSAMARDVKACSPQDTPATVQALMQQYKIRRVP
                     VVDQDRRLLGIVSLGDLAYVMSSQQTLGGDGMTWTAIAHTLAAVSAPRIPRYRR"
     gene            2689..3501
                     /locus_tag="VFQ35_08715"
     CDS             2689..3501
                     /locus_tag="VFQ35_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930755.1"
                     /translation="MAFSPFTEAIDAELDLVEGAATVARIEYPGLRAARVRARLDELA
                     APLARLGLARLPALAQARALADHLHVVNGFRGNADNYFDPSNSFINVVLERRLGIPIT
                     LAVVYVEVARRVGVSARGVGFPGHFLVRVDDRRDTVVLDPFFGGEVLDRVALGNLLQR
                     VAPRMTLRDEMLDPVSVKQLVARMLMNLRGIYAARADSGRLLAVLDHLIDLMPEAADE
                     VRDRGFLCARLGAPRAAVADLRRYVDALPHAGDVAEVRRAIERLEASAAPLH"
BASE COUNT          520 a         1114 c         1210 g          668 t
ORIGIN      
        1 gatcgttccg ctcgatctca gcttgaaatc cacggtcgcg cggggagaag gctatcgtct
       61 ggcggcgttc gggtcggtga gcggcgccgc cggcatcgag aacggcccgg cgtggctcgg
      121 gcgcgtcggt ttggtcgggc aagcctgctt cgacgtgcat tgtcgctcga gcgcgacgct
      181 gggcggcacc gctgtcctgc tcgggcccgc gacgttcatc gcgacggggg ccggggcggt
      241 gctccgcgtc agcgagctct tcgccatcct cgtcgaagtc gattcgctgg tgccggcggg
      301 acgaaatctc gccgactatc acggcatcat cggcggggtg ggctttcgct ggtccggtcg
      361 acgctgggcc gtcgacggcg ccttgtattc atcattgaat ggagaggggc cggtggttcc
      421 gttcttggcc gcgtcgtacc gcttcctgcg ttagagcccg cgactgactt cccattcgcg
      481 ggcccttggt gaatcgagcc gagctatgct gcgcgcgatg aaagaatcgt tgtcgctcgg
      541 cgtggatctg ggtggcacca agaccgaggc cgtggtcttg cggcgcgtcg cggcgccgca
      601 cccggatgac gcgcacttcg acgttttggc ccgagagcgc gtgcctaccc ccagtagtgc
      661 cggctacgac gccgtgctcg cggcgaccgc agagctcgtg cgccaagtgc tcgcgcaggc
      721 tggtctctcg gcgcgtgaaa cgtcgattgg cgtcggcatg ccgggcgcgg tcacgcgccg
      781 cgagggcttg gtgaagaact cgaattcggt gtgtctcaat ggccgcccct ttcgcgaaga
      841 tctgagccgt gcgctcgggc agacggtggc cttcgagaac gacgcgaact gcttcgcgct
      901 cgcggagacg gagctcggag caggtcgcgc tcatcgagac ggcatcgtgt tcggagtcat
      961 catgggcacc ggcgtgggcg gtgggctcgt cgtccatggc cgcgtgtggc cgggccttca
     1021 gagcctcggg ggagaatggg gacatcacgc ggtcgggcct tggcgccgcg ccggtgcagc
     1081 ggaagccccc ggtgagtttg gtctcggact tccggcgctc ccggtttgtt actgtggaaa
     1141 gacaggttgc ctggagctct acgcggccgg gccggcggtg gagcgcgact acgcgaaccg
     1201 aagcggcgag cgccgctcgc tgcgcgaaat tgcggagcga caccgcgaag acgctcacgc
     1261 cgcggcatcg atcgaccagc tgctcgaagc gttcggtcgt ggcctcgcca acgtgatcga
     1321 catcgtcgat ccgagcgcga tcgtccttgg tggcggcgtc tcgaacctat cgtgcttgta
     1381 cgacgagggc gtggatcgag ttcgccgcta cgtgttcaac gacgagctcc tcacgccgat
     1441 tctcaagcac gagctcggcg actcggcggg cgttctcggg gcggcgcttc tgaacggcga
     1501 ctgactactg ccggtgatac tcggcttcga gaaattgctg ccaggtctgc ccgccgtcct
     1561 gggtgttctc gatggtgaag aggtagctgt cttcgtcgtt gaagcggtac gtgtagcggc
     1621 cgcgctgcgg cgccgtcgtg tcgtgctcga acacgagcgt gttgccctcc caacgtccgc
     1681 gtgacggagc tgaaggagcc ataccgagcg agtccaccca gtaccaggtg tacgtcttct
     1741 tctgttcgtc ccagccgaag acgccgtggc cgcggtagac gacgcgcccg tctttctctt
     1801 cgacgtagtc ctgaatgacg aaaaagccgt ccacgtcgac gcgtccgatg tagcgcccga
     1861 tggcaatacc tccggggccc cactcggaag ggctcaggtg ctcctcaccg atccaagttc
     1921 ctgcgagata gtggagctta cggtggtgtt cgctcggctc gggcattccc atcatggcgc
     1981 gactccctca atgcgaaatt cgactcttag caggcaaggg cctagcgacg atagcgtggg
     2041 attcgtggag cgctgacggc cgctagcgtg tgtgcgatag ccgtccacgt catcccatcg
     2101 ccgcccaacg tttgctgcga gctcatgacg taggcgagat cgcccagact cacgatgccg
     2161 agcagccgcc tgtcctgatc caccaccggg acacggcgga tcttgtactg ctgcattaga
     2221 gcctgaacgg tagctggcgt atcttgcggg gaacacgcct tcacgtcgcg cgccatcgcg
     2281 ctcgacacgc tggaatcgct caacgcaacg ccctggagct gcgctgccat ggcgaggtcg
     2341 cgatcggtga tgagtcccac gagacgaccg tcgcgatcca ccaccggcac tgcgccgcag
     2401 tcgttctccc acatgatctg cgccgcggtc gccagggtgt cagtcggagt gcaactgtgc
     2461 acgtctcgcg tcattatttc tgcgatcttc acgggcatct cctttcgccc ggtcgggccc
     2521 ggactcaggc gcggccggag cgcgccaggg caaagcttac tcagctcgcc tgcatttcgc
     2581 gagggcgtcc gacttcgaag cgttcgttgg cgcgcgcttt tttaaggcgg cgcggcggcc
     2641 ggggcctgaa aaggggtcgg ctgcagggtc ggcgggtata ctgccggagt ggcgttctcg
     2701 ccctttaccg aagcgatcga tgcagagctc gacctcgtcg agggagccgc gaccgtcgcg
     2761 cgcatcgaat accccggact tcgagcagct cgcgtgaggg ctcgcctgga tgagctcgcg
     2821 gctccgctcg cgcgcctcgg gctcgcgcgg ctaccggcgc tcgctcaggc tcgggccttg
     2881 gccgaccacc tacacgtcgt gaacgggttt cgaggaaacg cggacaatta cttcgatcct
     2941 tccaactcgt tcatcaacgt cgtgctcgag cgacggctcg ggatcccaat cactcttgcg
     3001 gttgtttacg tggaagtggc gcgtcgggtt ggggtttcgg cgcggggcgt agggttcccg
     3061 gggcactttc tggtgcgagt ggacgaccgg cgcgacaccg tcgtgctgga tccgttcttt
     3121 ggcggggaag tattggaccg ggtcgcgctc ggcaacctgt tgcagcgcgt cgcgccgcgc
     3181 atgaccctgc gcgacgagat gctcgatcca gtcagcgtga agcaactcgt ggctcgcatg
     3241 ctcatgaatc tgcgcggcat ctacgccgcg cgcgccgact cgggccggtt gctcgccgtg
     3301 ctggaccacc tgatcgactt gatgccggag gccgcggacg aagttcgaga ccgaggcttt
     3361 ctatgcgctc ggctcggcgc cccacgcgcc gcggtagcgg atcttcgccg ctacgtggac
     3421 gcgttgccgc acgcggggga cgtggccgag gtgcgcaggg ccatcgagcg gctcgaggcg
     3481 agcgccgcgc cgctgcattg agcggctacg gg
//
LOCUS       DASTKS010000416         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_734351, whole genome shotgun sequence.
ACCESSION   DASTKS010000416 DASTKS010000000
VERSION     DASTKS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734351"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1007
                     /locus_tag="VFQ35_08720"
     CDS             78..1007
                     /locus_tag="VFQ35_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930756.1"
                     /translation="MSHRELLTLIAQIAGIPSADVQLLSRVLGVPLVQLGPPGRWSEF
                     AANGGTRAFQSASAKLDVEQGFGSVRLKPHSPPPTRAELGIFSDPTTYRLEVNPNIPP
                     RGVTTYVSSQRGGALEVRYVFGSGSDALLDAIVSWQSVRVPQGEEAVFRTAPPLIYLR
                     SYDSVDEVLEYACATRAWRILRREESSALGATEAFGAFARGVPAKSGAGELMGGVFAT
                     PEGPAIIAGSKRSLVQPVKTQARIDQLPERRRRFSLTRAGLHELELIYEERHGVGTKP
                     YDVEESDVDLFVAIRDGLKKERWLAAHTRPWPG"
     gene            1237..>2261
                     /locus_tag="VFQ35_08725"
     CDS             1237..>2261
                     /locus_tag="VFQ35_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930757.1"
                     /translation="MTRSLALLSIGLMSSACGLSGGDDSGNASGGNNPIGFGGASSSS
                     SGGSTSSSSGGGVSVGTGGGSGVSITDLESRACAGTKIEPESLPAVMQIVLDTSLSMN
                     STTPATNGQSKWTVTRAALEETIDNLPANVAVGLLYFPNKDTSAGTTPRDVSACVNVD
                     ALVPIALLDAPNSAARRALHDSLERTSPAGSTPTHDAYTYGLNESLLKTKAPGARFML
                     LITDGGPTLRLECLGEGTLQYPQPTQPIIDSVQAAYIDQHVKTFLVGVPGTEDIGNQT
                     HDDGRPWMSKAAVVGGSAVSGCNVFGPNFCHIDLTQAADFSAALRRGLGYISRQIISC
                     SYEVPTHS"
BASE COUNT          430 a          728 c          723 g          380 t
ORIGIN      
        1 agactgtaca tcatgggctg taacgaagga gagcgtagtg cggggcgacc cgcgactcca
       61 agtgataggc aggcacgatg agtcatcgag agctgttgac gctaatcgcc caaatcgcgg
      121 ggattccgag cgccgacgtc cagttgctca gtcgggtgct cggggtgccg ctcgtccagc
      181 tcggcccacc cggacgctgg agcgagttcg ctgccaacgg cgggacgcgc gcgtttcaga
      241 gcgcctccgc gaagctcgat gtcgagcaag ggtttggctc cgtacggctg aagccgcatt
      301 ccccgcctcc gacgcgcgca gagctcggta tcttctccga cccgacgact taccgactcg
      361 aagtgaatcc aaacattccg ccgcgagggg tgacgaccta cgtctcgtcg caacgcggcg
      421 gagcgctgga agtccgctac gtcttcggca gcggaagcga cgccttgctg gacgcgatcg
      481 tcagctggca aagcgtgagg gttccccaag gggaggaagc cgtgttccgc acggccccgc
      541 cgctcatcta ccttcgcagc tacgactcgg tggacgaggt tctcgaatac gcctgcgcaa
      601 cgcgcgcctg gcgcattttg cgtcgcgaag agtcatcggc cctaggcgcg accgaagcgt
      661 tcggcgcgtt tgcgcgcggt gtccccgcca agagcggagc gggtgagctg atgggtggcg
      721 ttttcgcgac tccggaggga ccagccatca tcgccgggtc caaacgctcg ttggttcagc
      781 ccgtgaaaac gcaagcccga atcgaccaac ttccagagcg acggcgccgg ttcagcctaa
      841 cgcgcgccgg cctacatgaa ttagagctca tttacgagga gcgtcacggc gtcggaacga
      901 aaccctacga tgtcgaggaa tcggacgtcg acctcttcgt cgcgatccgt gacgggctga
      961 agaaagagcg ctggctcgcc gcgcatacgc gcccgtggcc cggctgaaag aattgggcga
     1021 aaagcgagca aaaatctttc tttgtggagc acacgcgacc acacctcctc aagaaaattt
     1081 tcgagctgct gtcgggtcgt gtcgaccggc gggcattgcg gtagagtgag ctctcgtgca
     1141 gacacttcgc gcgcgacgag gagctgcccc ccgcgcactc tcatgcctca tgagagccga
     1201 acagccgcgt tcgaccgcca ccgtcggagg aacctgatga cccgatctct cgcactcttg
     1261 tcaattgggc tcatgagctc agcctgcggc ctttccggag gtgacgactc cggcaacgcg
     1321 agcggaggga acaacccgat cggatttggc ggcgcgagca gcagcagctc gggcggttcg
     1381 acgagctcga gctctggcgg gggcgtctcg gtcggcaccg gcggtggaag cggcgtaagc
     1441 atcacggatc tcgagagccg cgcctgcgcg ggcaccaaga tcgagcccga aagcttgccg
     1501 gcggtgatgc agatcgtgct cgacacgagt ttgtcgatga actcgaccac tccggcgacc
     1561 aacggtcaga gcaagtggac ggtcacgcgc gcagccctcg aggaaacgat cgataacctc
     1621 cccgcgaacg tcgcggtggg gttgctctac ttccccaaca aagacacgag cgcagggacg
     1681 actccgcgcg acgtctcggc gtgcgtcaac gtcgacgcgc tcgttccaat cgcgctgctg
     1741 gacgcgccga attctgccgc gcggcgcgcg ctgcacgact cgctcgagcg tacgtcaccg
     1801 gccggctcga ccccgacgca cgacgcctat acctacggct tgaacgagag cctcttgaag
     1861 accaaggcgc ccggtgcgcg cttcatgctg ctcatcaccg acggcggtcc caccttgcga
     1921 ctggaatgcc tcggtgaggg cacgctgcaa tacccccaac ccactcagcc gatcatcgac
     1981 agcgttcagg ccgcctacat cgaccagcac gtgaagacct tcttggtggg ggtgcctggc
     2041 acggaggaca tcggcaatca aacgcacgac gacggcaggc cgtggatgtc gaaagcggcg
     2101 gtcgtaggtg gctcagcggt ctccggttgc aacgtgttcg ggcccaactt ctgtcacatc
     2161 gatttgacgc aggcggccga cttttcggcc gcgttgcgcc gcggactcgg gtacatctca
     2221 cggcagatca tctcgtgtag ctacgaggtg ccgacgcact c
//
LOCUS       DASTKS010000417         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1405160, whole genome shotgun sequence.
ACCESSION   DASTKS010000417 DASTKS010000000
VERSION     DASTKS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405160"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..935)
                     /locus_tag="VFQ35_08730"
     CDS             complement(174..935)
                     /locus_tag="VFQ35_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930758.1"
                     /translation="MPGPPPSRPSSPSSSGEPSLRRLGVGSSGPERPVRAQLVVALVA
                     AVTLAAVPLYLMRHPSANKRVEADALASASASTSALALASAAAPAPIIKPPERIRLGT
                     PVRVRCGSNPKRGSEGNLCESLVPLEEALAKAIRENENCASRLKQTGTINYVLNVDFA
                     KKTLHVFPGASGDYRGPQARRITTCIEHALAKPDWDSIRHQYRYYTFAIMTTYTPDAP
                     VVPAAPAMPPSVPPLTGSPTPAPAPATTSQGPRFD"
     gene            complement(944..>2385)
                     /locus_tag="VFQ35_08735"
     CDS             complement(944..>2385)
                     /locus_tag="VFQ35_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930759.1"
                     /translation="LAPSPWGPILVMEQIAGQTLHTHIRKRKAQGGYSADEFRKTASE
                     SAAGLAAIHAQGLVHGDLKPGNVMVTNDRAIILDFGFAQERARLSARRPGAPPDGGTP
                     NYMSPERLRSGGASPEDDVYALGLTLWEMWTCRVPEPGYRPRSKPMRAQIMFDVPAGL
                     SVDEVKQVFRCLSDDPAMRPQARHLRFFNPSSLTTSQVQIPRERISPGAPPGRVTAET
                     FVPGSQSLLVTYCTNSPEAAGLLFPLNKPLITIGRRAGQDIVMPEATVSGAHAVLKWQ
                     SGSWTIEDTGSTNGTYAEHSYERKKTSALLHGGEVQLGESRVKLVSFSDGSPQHERAK
                     AYLARRDGLTGLLRREHLLRAVEEEMAFAEWSNNPLSVARYHVRSPNRHVSDRPTILE
                     MLALRRAAQRVLELTEMLLLSIVPVTGGRIGPLKFAVVMTGPSLDEARNVVEQVVSQV
                     QGSMPDSLDLGATLVRAEPGQRVQALLDE"
BASE COUNT          389 a          734 c          888 g          374 t
ORIGIN      
        1 cccggagcag cgaacccgga gcggcgaacc cggagcagcg aacccagagc ggcgaacccg
       61 gagcagcgaa cccggagcgg cgaacccgga gcagcgaacc cggagcggcg aacccggagc
      121 ggcgaacccg gagcggcgaa cgcagacccc ggatgggaag tgcacccgac tcgttagtcg
      181 aagcgggggc cttgagaggt ggtggcaggc gctggcgcgg gggttgggct gccggtgagg
      241 ggcgggaccg aggggggcat ggcgggcgcg gcggggacga cgggggcatc gggcgtgtag
      301 gtcgtcatga tcgcgaaggt gtaatagcga tactggtgac ggatgctgtc ccaatcgggc
      361 ttggccaaag cgtgctcgat gcaggtggtg atgcggcgcg cttgcggccc gcggtagtcg
      421 ccgctcgcgc cggggaagac gtggagggtt ttcttggcga agtcgacgtt gagcacgtag
      481 ttgatggtgc ccgtttgctt cagccgcgac gcgcagtttt cgttttcgcg aatggctttg
      541 gcgagcgctt cttcgagggg aaccaacgac tcgcagaggt tgccctcaga gccgcgcttt
      601 gggttcgagc cgcaacgaac gcgcacgggg gtgcccaagc gaatgcgctc gggtggcttg
      661 atgatgggcg ccggcgcggc cgcgctcgcg agcgccaacg cggacgtgga ggctgacgcg
      721 gaagcgagag cgtctgcttc gacgcgcttg ttcgcgctcg gatgccgcat caggtagagc
      781 ggcaccgccg cgagcgtcac tgccgccacg agcgcgacga ccagctgcgc gcgcactggg
      841 cgctcggggc cggagctgcc cacgccgaga cgccgcagcg acggctcacc ggagctcgaa
      901 ggagaggacg gtcgcgaagg cggcgggcca ggcatgcgtt gggctattcg tcgagcagcg
      961 cctgcacgcg ttgtccgggc tccgcacgca cgagcgtcgc accgagatcg agcgagtcag
     1021 gcatcgagcc ctgaacctgg gagaccacct gttcgaccac gttgcgcgcc tcgtcgaggc
     1081 tcggaccggt catcacgacc gcgaacttga gcggaccgat gcggccgccc gtgaccggca
     1141 cgatactgag caaaagcatc tcggtgagct cgaggacgcg ctgcgcagca cgccggagcg
     1201 cgagcatttc gaggatggtt gggcgatcgc tcacgtggcg attcgggctc cgcacgtggt
     1261 agcgcgcgac cgagagcggg ttgttcgacc actcggcgaa ggccatctct tcttcgaccg
     1321 cgcggagcag gtgctcgcga cgcaagaggc cagtgagccc gtcgcgccga gcgaggtacg
     1381 ccttcgcgcg ttcgtgctgg ggcgagccgt cactgaagct cacgagcttt acgcgcgatt
     1441 cgccgagctg aacttcgccg ccgtggagca gcgcgctcgt ctttttgcgc tcgtagctgt
     1501 gctcggcgta ggtgccgttg gtgctccctg tgtcctcgat ggtccaggaa ccggactgcc
     1561 acttgagcac ggcgtgcgcg ccgctgacgg tagcctccgg catcacgatg tcttgcccgg
     1621 cgcgccgccc aatcgtgatg agcggcttgt tcagcgggaa caagagcccc gccgcctcgg
     1681 gagagttggt gcaatacgtg acgagcagcg attgggatcc gggaacgaag gtctcggcgg
     1741 tgacacgccc gggaggcgcg ccgggagaga ttcgctcgcg cgggatttgc acctggctcg
     1801 tggtgagcga gctcgggttg aaaaagcgca ggtgacgcgc ttgcgggcgc atcgcagggt
     1861 cgtcgctcaa gcaacggaac acctgcttga cctcgtcgac ggagagcccc gcgggcacgt
     1921 cgaacattat ctgcgcgcgc atcggcttcg agcgcgggcg gtacccgggc tcgggcacgc
     1981 ggcacgtcca catttcccaa agcgtgaggc cgagcgcata gacgtcgtcc tcggggcttg
     2041 ctccaccaga gcgcaaacgc tccggcgaca tgtagttggg agtgccgccg tccggtgggg
     2101 caccggggcg acgcgcactc aggcgagcgc gctcttgagc gaaaccgaag tccaggatga
     2161 tggcgcgatc gttcgtcacc atcacgttgc cgggcttcaa gtcgccgtgt acgagccctt
     2221 gcgcgtggat cgcagcgagg cccgcggcgc tttcgctagc ggtctttcga aactcgtccg
     2281 ccgagtagcc gccttgcgcc ttgcgcttgc ggatgtgcgt gtgcagcgtt tggccggcga
     2341 tctgctccat cacgaggatg ggaccccaag ggctcggggc gagat
//
LOCUS       DASTKS010000418         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_66943,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000418 DASTKS010000000
VERSION     DASTKS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66943"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFQ35_08740"
     CDS             complement(<1..1104)
                     /locus_tag="VFQ35_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9930760.1"
                     /translation="MAELNPAQNEAVHTLSGPLLVLAGAGTGKTRVITFRIAQLIKTG
                     ITASRILAVTFTNKAAKEMRERAQHLLGRRQPGTKPPEISTFHSLCVRILRRHAHRLG
                     YPETFSIYDRGDQETLARNALRDIRVSSGTMRPGDLLSILGGWKNKGVSVDEAHQTAE
                     GERYEVAAAAYARYQAQLKAAGAMDFDDLLLMTNRLFAEHPEARFAEATRFDHLLIDE
                     YQDTNDLQYRIVKVLAERHRNLCVVGDDDQSIYGWRGAEVAHILGFQNDWPDAKIVRL
                     EANYRSRAPIIQLANTLIAHNSTRHQKVLRPSREGGVAPRILRMEDETMEAEHVVQQI
                     QQLIVGDAEKRTPPSDIAILFRTNEQPRAFELEM"
     gene            1209..1988
                     /locus_tag="VFQ35_08745"
     CDS             1209..1988
                     /locus_tag="VFQ35_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9930761.1"
                     /translation="MPLHVERSGTGPTLVLAHGFAGSARNFRPQARAFADRVHTVLYD
                     MRGHARSSAPEDTSEYTEDALVSDFGSVVEGAGGAAIVGGLSLGAYTALLYALSASTP
                     PKGLLLSAFPSGGATGFRSRWSRDFAEAIERDGLESAGERFVWGETSRFDPKGAQLIR
                     QGFLEHPPHALAALLRNVLSHLKTPDELAERLSSFSAPTLVIVGSEDQDSLGPSHRLA
                     ELLPNAELVIVPSAGHVVNLAAPAAFNDALAKLVERALSSS"
     gene            complement(1975..2451)
                     /locus_tag="VFQ35_08750"
     CDS             complement(1975..2451)
                     /locus_tag="VFQ35_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930762.1"
                     /translation="MTFPSAPQHAQGGGPFGPPGSGYGSGMPPGGYGPPGAYGPPGHP
                     GYPPPAGNPPPPGFHVPPPAPGPGADLKKQATNWLVAAAVTFFFCGGINCFGVIGAIL
                     CFLAMQAADQANLSDAESKLKWGKIVIVTGAIVSFITIAFAILYYFVAQAEGLTTS"
     gene            2672..>3365
                     /locus_tag="VFQ35_08755"
     CDS             2672..>3365
                     /locus_tag="VFQ35_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930763.1"
                     /translation="MASPLARGDHVVVEARAAEFFEGRVLSIGKSQLRVEPAGKHEGV
                     LVSSSDAYPLKASDAVAKVGQFAICRIGGGWTGCRVEQVHADDLTVRTLEGDTAHLRR
                     SDLVAASAVTELNLRRAFGKLSSRAEFARAAAHAGRPVAPAGHRITPRSRVVALRASG
                     WYSGVVADVHDDGAHVLFAPDNVSEQIALADVVPEPPASAAPGRGDFALVRPPSPAEA
                     WQVMRVIGPEDRA"
BASE COUNT          493 a         1071 c         1187 g          614 t
ORIGIN      
        1 catttcgagc tcgaaggcgc gcggctgctc gttcgtgcga aacaagatcg cgatgtcgct
       61 cggcggagtc cgcttttcgg cgtcgccgac gatgagctgc tgaatttgtt ggacgacgtg
      121 ctccgcttcc atcgtctcgt cctccatgcg gaggatgcgt ggcgcgacgc cgccctcgcg
      181 cgaggggcgc agcaccttct ggtggcgcgt gctgttgtgc gcgatgagcg tgttggcgag
      241 ctgaatgatg ggggcccggg agcggtagtt cgcttcgagg cgcacgattt tggcgtcggg
      301 ccagtcgttc tgaaagccga gaatgtgcgc gacttcggcg cctcgccagc cgtaaatcga
      361 ttggtcgtcg tcgccgacga cgcaaaggtt ccggtgccgc tcggccagca ccttgacgat
      421 gcgatactga agatcgttcg tgtcctggta ctcgtcgatg agcaggtggt cgaaccgcgt
      481 cgcctcggcg aagcgagcct ccgggtgctc ggcgaagagg cggttggtca tcaacaagag
      541 gtcgtcgaaa tccatcgccc cggcggcttt gagctgggct tggtagcgcg cgtaggcggc
      601 ggcggcgacc tcgtagcgct ccccctcggc ggtctggtgc gcctcgtcga ccgatacgcc
      661 cttgttcttc cagccaccca agatggagag caagtcaccg ggtcgcatcg tgccgctgct
      721 cacgcggatg tcgcggagcg cgttgcgagc caaggtctcc tggtcgcccc ggtcgtaaat
      781 cgagaacgtc tctgggtagc cgagccggtg cgcgtggcgc cgcaggatgc ggacgcaaag
      841 cgagtgaaag gtggagatct ctggcggctt ggtccccggt tgacggcggc cgagcaggtg
      901 ctgggcgcgc tcgcgcattt ctttggcggc cttgttggtg aacgttacgg ccaagatgcg
      961 cgacgcggtg atgccggttt tgatgagctg cgcgatgcga aaggtgatga cgcgcgtttt
     1021 gcccgtcccg gcgccggcca acaccaacag aggtcccgag agggtatgta ctgcctcgtt
     1081 ttgggctggg ttcagctcgg ccacgcgctc caattacgga ccgaagcggg ctcggtcaag
     1141 gccgagcggt gcattggctg gtgcgatcct tgcttgtgcg atagatccgg tgcgctttga
     1201 ctcgctcggt gccgctccac gtcgaacgtt ccggaactgg gccgacgctc gttctcgcgc
     1261 atggcttcgc cgggagcgcg cgaaacttca ggccgcaggc gcgtgcgttc gcggatcgag
     1321 tgcacacggt cctctacgac atgcgaggcc acgcccgcag ctcggctccg gaggacacct
     1381 ccgagtacac ggaggacgcc ctcgtctccg attttggttc tgtcgtcgaa ggcgcgggcg
     1441 gcgccgcgat cgtcggaggg ctgagccttg gtgcttacac ggccctgctc tacgcgctct
     1501 ccgcgtcgac gccgccgaag ggtctattgc tctcggcgtt tccgagcggc ggcgcgaccg
     1561 gcttccgctc gcgctggtcg cgggatttcg ccgaggccat cgagcgggac gggctcgaga
     1621 gtgccggcga gcgcttcgtg tggggcgaga cgtcgcgctt cgatcccaaa ggcgcgcagc
     1681 tcattcggca aggttttctg gagcatccgc cgcacgccct cgccgcgctg cttcgcaacg
     1741 tgctgagcca cctgaagacg ccggacgagc tcgccgagcg actgagctcg ttttccgcgc
     1801 caacgctcgt catcgtcggt tcggaggacc aagactccct cgggccgagc caccgcctcg
     1861 cggagctctt gccgaacgcg gagctcgtca tcgttccttc cgctgggcac gtcgtgaact
     1921 tggctgctcc ggcagcattc aacgacgcgc tcgcgaagct cgtggagcgc gcgctcagct
     1981 cgtcgtaagg ccctcggcct gcgcgacgaa gtagtagaga atcgcgaacg cgatggtgat
     2041 gaacgacacg atcgcgccgg tcacgatcac gattttgccc cacttgagct tggattcggc
     2101 gtcgctcagg ttcgcctgat ccgcggcttg catcgcgagg aagcacagga tcgctccgat
     2161 cacgccgaaa cagttgatgc cgccgcagaa aaagaaggtg accgccgcgg ctacgagcca
     2221 attcgtcgct tgcttcttga ggtcagcgcc ggggcccggg gccggaggtg gcacgtggaa
     2281 gcccggtggc ggcgggttgc ctgcgggcgg aggatagccc gggtgccctg gagggccgta
     2341 cgcacccggc gggccgtaac cgccaggcgg catgcccgag ccgtagccgg agcccggcgg
     2401 accaaagggt ccaccgcctt gagcatgctg gggagctgag gggaacgtca tcccgttgtc
     2461 cgttgcggtc cgcgagactt gcgcagccag cgccgtcctc gtatcagcgg ggggagcgta
     2521 gaacaccctc gaacgcatgc gccacccatt cgtgctccag cacgcgccgg ctcggcttgg
     2581 aatgacgtcg ttccgccgga cagcgtggct cgccttggtc ttcacggcct gggcctgccg
     2641 ttcggaggga ggcggttcgg ctccttcgcc cgtggcgagc ccactcgcgc gaggtgacca
     2701 cgtcgtggtg gaggcgcgtg cggcggaatt cttcgaaggg cgggtgctct cgattgggaa
     2761 gagccagctg cgcgtcgagc ctgcgggaaa gcacgaaggc gttctggttt cttcatccga
     2821 tgcctacccg ctgaaggcgt cggacgcggt cgcgaaggtg gggcagttcg cgatatgccg
     2881 catcggcgga gggtggacgg ggtgtcgagt cgagcaagtg cacgccgacg acctcacggt
     2941 gcggactctc gagggtgata cggctcactt gcgccgcagc gatctcgtgg ctgcttccgc
     3001 cgtcaccgag ctgaacttgc gccgcgcatt cggcaagctc tcgtcgcgtg ccgagtttgc
     3061 gcgagccgct gcgcatgcgg gccgtcccgt cgctcctgcc ggccaccgaa tcacgccgcg
     3121 cagtcgcgtc gtcgcgctga gagcatcggg ttggtacagc ggcgtggtcg ccgacgtgca
     3181 cgacgacggg gcccacgtgc tgttcgctcc cgacaacgtc agcgagcaga tcgcgctcgc
     3241 ggacgtcgtg cccgagcccc cggcgagcgc ggcccctggc cgcggcgatt tcgcgctggt
     3301 gcgtccgcct tcgcccgcgg aggcctggca ggtcatgcgc gtgatcggcc cggaagatcg
     3361 agctt
//
LOCUS       DASTKS010000419         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1851881, whole genome shotgun sequence.
ACCESSION   DASTKS010000419 DASTKS010000000
VERSION     DASTKS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851881"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFQ35_08760"
     CDS             complement(<1..577)
                     /locus_tag="VFQ35_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930764.1"
                     /translation="MLHGRNPYLEFGPAEDWVAWTALGLAFVGAALALSKSSVERLAR
                     VPSRALLALLGVAATLLSFGYVAHYLRGGPRIVDATYYFLAGRALAHGDFAFEVPGPL
                     ASFAGRFLLAAPNRHALGVLFPPGYPAALALALKLGAPLALGPALAAALVLVTYRLAK
                     RVGEKEDVARAAAALSVLSAALRYHTADTMSH"
     gene            complement(597..2753)
                     /locus_tag="VFQ35_08765"
     CDS             complement(597..2753)
                     /locus_tag="VFQ35_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930765.1"
                     /translation="MPEARTTMGARARRYEALTYTLIAATLTLAVACKFYGSLRLQTL
                     HALNWSDPEAFEKWVSKGRGPWSAPLDDVFIHFDFARSAARGFPFQWSEGNGYSSGGT
                     SLLYPLVLAIGYRAGLRELDLMQWAAIVACVGTFGLLLGARRLTRELPRAASYLLPFG
                     LLSVGVLNWSLFSGMEVALFLGLWALAYVAYDDVLLFEGTDDRRALLVRAALLGACCG
                     LVAATRPEGITAVAVLVGAACIHVVRRRDFRTALLVGALGAGPALLVLVAQTLANRVF
                     TGETTAAGALVKLELHHPYLDARAVWDSWLFFIKYQVLRVTQYHFGDQPAYGWLAWAL
                     AACSLAFRSTRRPALVLWASAIAWVSMVAMNGQVRWQNERYTMPAVAWLLAAATLGTA
                     GLLQRAYELRSRMRGPLLAAATAAAVTLFFIHQAPRYREQLWFFGRAARNIYDQHVQA
                     ALVLRDQITPQPRRVLVGDAGAIPYISDLPALDIIGLGGYHAMPFARATRMHVGAAIE
                     LLERMTPDERPDMLAIYPGWWGDFPAWFGRPVAAIPVRGNVICGGAAKVLYRPDWSPL
                     DASARPFSLRAGERVLDSLDEADLVNERNHGYRVVGAPGFVSMELIEGPMDKKPLWDA
                     GRTLPAAAYETFTLSGDERAHNARLVVRAAPVERTNIDVSVDDEPIGKLVLEPRGGWV
                     EGTISHSRLGARKVRIKLGGSDRDRLLYHVFLVAEP"
     gene            3118..3381
                     /locus_tag="VFQ35_08770"
     CDS             3118..3381
                     /locus_tag="VFQ35_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930766.1"
                     /translation="MRWFVEVSRVGDSTPAERYCVEAKAWQAALQETRKLRGDTGPLS
                     KFSIELLDDGYRAVDPIQKIRYLVNQAPSDAPIALPVNGVAHA"
     gene            complement(3744..4094)
                     /locus_tag="VFQ35_08775"
     CDS             complement(3744..4094)
                     /locus_tag="VFQ35_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930767.1"
                     /translation="MKTRCSGIATAFDGASALTGERGAFATEGVAARRADSLPMVLTG
                     AFGAAERASSAADEAAADAGTDADAAALGAPLGAITATGATATGAAAGAALPFAAADS
                     MGATSGGRGVLSAL"
     gene            4093..>4398
                     /locus_tag="VFQ35_08780"
     CDS             4093..>4398
                     /locus_tag="VFQ35_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930768.1"
                     /translation="MSRSQEPKPDAPITYREQACVVAPGTPREAVEALLRERWQSVKK
                     EIATHPSGQLVQIAVFDHVFEQRPQRSPLGTLAWKDWRGDPVLGFPGFGVPSPPVTKS
                     "
BASE COUNT          784 a         1491 c         1467 g          656 t
ORIGIN      
        1 cgtgcgacat cgtgtcggcc gtgtggtagc ggagcgcggc cgagagcacc gagagcgcgg
       61 cggcagcgcg cgcgacgtcc tctttctcac cgacgcgctt cgcgagtcga tacgtgacga
      121 gtacgagcgc ggccgcgagc gctgggccga gcgcgagcgg tgcgccgagc ttgagcgcga
      181 gcgcgagcgc cgcgggatat ccgggaggaa agagcacgcc gagcgcgtgt cggttcggtg
      241 ccgcgagcag aaatcgcccc gcgaaggacg ccaacgggcc tggtacttcg aaggcgaagt
      301 cgccgtgcgc gagcgcgcgc cctgcgagaa agtagtaggt cgcatcgacg atgcgcggcc
      361 ccccgcgaag gtagtgcgcg acgtagccga acgagagcag cgtcgcggcg acgccgagca
      421 gcgcgagcag cgcgcgcgac ggcactcgag cgagccgctc cacgctgctt ttcgaaagtg
      481 cgagcgctgc gccaacgaac gcgagcccga gcgccgtcca ggccacccaa tcctcggcgg
      541 gaccaaactc gaggtagggg tttcgaccgt ggagcatgct ggctgcgctc tcggctctac
      601 ggctccgcaa ccagaaagac gtgataaagg agtcgatctc gatccgaccc gccgagcttg
      661 atgcgcactt tacgagcgcc gagccgcgag tgcgagatgg tgccttctac ccagccgcct
      721 ctcggctcca aaacgagctt gccgattggc tcgtcgtcga cgctcacgtc gatgttggtg
      781 cgttcgacgg gcgcagcgcg cacgaccaaa cgcgcgttgt gcgcgcgctc gtcgccgctc
      841 agcgtgaacg tctcgtaggc ggcggcgggc agagttcgac cggcgtccca gagtggcttc
      901 ttgtccatgg gcccttcgat gagctccatc gagacgaagc ccggagctcc cacgacgcgg
      961 tagccatgat tgcgctcgtt gacgagatcc gcttcgtcca agctgtcgag cactcgctcg
     1021 cccgcgcgca gcgaaaacgg ccgcgcgctc gcgtcgaggg gcgaccaatc ggggcgatac
     1081 aagaccttcg ccgcgccgcc gcagatcacg ttgccgcgca cgggaatggc ggccacgggg
     1141 cggccgaacc aagccggaaa atctccccac cagccagggt atatcgcgag catgtcgggg
     1201 cgctcgtcgg gcgtcatgcg ctcgagtagc tcgatggcgg cgcccacgtg catacgcgtc
     1261 gctcgcgcga aaggcatggc gtggtagccg ccgagcccga tgatgtcgag cgccggcaaa
     1321 tcgctgatgt aggggattgc gccggcatcg ccgaccaaaa cccgccttgg ctgcggtgtg
     1381 atctggtcgc gcaagacgag cgcggcttgc acgtgctggt cgtagatgtt gcgcgcggcg
     1441 cgaccgaaga accaaagctg ctcgcgatag cgcggagctt gatggatgaa gaacaacgtc
     1501 accgcggccg cggttgcggc ggcgagcaga ggcccgcgca tgcggctccg gagctcgtag
     1561 gcgcgctgaa ggagccctgc cgtaccgagc gtcgccgccg cgagcagcca cgcgaccgcg
     1621 ggcatcgtgt agcgctcgtt ttgccagcgc acttgcccgt tcatcgcgac catcgaaacc
     1681 cacgcgatgg cgctcgccca caagacgagc gcgggccgtc gcgtcgagcg aaacgccagc
     1741 gagcaagctg cgagcgccca cgcgagccag ccgtacgctg gttggtcgcc gaagtgatac
     1801 tgcgtgacgc gcagcacctg atatttgatg aagaagagcc agctgtccca aaccgcgcgc
     1861 gcgtcgaggt acgggtgatg aagctcgagc ttgacgagcg cgccggcggc cgttgtttct
     1921 ccggtaaata ctcggttcgc cagcgtttgc gcgacgagca ccagcaacgc cggacccgct
     1981 ccgagcgcgc cgacgagcaa cgccgtgcgg aaatcgcggc ggcgcacgac gtggatgcac
     2041 gcggccccga ccagcactgc gaccgcggta ataccttccg gtcgagtagc cgcgacgagc
     2101 ccgcagcagg cgccgagcaa ggcggctcga accagcagcg ctcgtcgatc gtcggtgccc
     2161 tcgaacaaaa gcacatcgtc gtaggcgacg tacgccagag cccacaggcc gaggaagagc
     2221 gcgacttcca tgccgctgaa gagcgaccag ttcagcacgc cgaccgacag caacccgaag
     2281 ggcaacaagt agctcgccgc ccgcggcagc tcgcgcgtca agcgacgcgc gccgagcaac
     2341 aatccgaacg ttcctacgca ggccacgatc gcggcccact gcatcaggtc gagctcacgc
     2401 agcccggcgc ggtagccgat ggcgagcacc agtggataca agaggctcgt cccgccgctc
     2461 gaatacccat ttccctcgct ccactggaaa ggaaagccgc gcgcggccga ccgggcgaag
     2521 tcgaagtgaa tgaagacgtc gtcgagcggg gccgaccagg gcccacggcc tttggaaacc
     2581 cacttttcga atgcttccgg atcggaccag ttgagcgcgt gaagcgtttg caatctgagc
     2641 gaaccgtaga acttgcacgc aaccgccagg gtgagagtgg cggcgatgag cgtataggtg
     2701 agcgcctcgt agcgacgggc ccgagcgccc attgtcgtcc gggcttccgg catgggaccg
     2761 gcgatctatc accgaacctc gcgctgacga tggccgatcg cgttcagaaa cacagcgacc
     2821 caccgccacg cactggatcg ctgttcggtg cgtacccgtg aaagcttcga aacaggcagc
     2881 cggtctcccg aaatcccgag cagaactcgg ggcgtgcaat gcttgccgaa ccgcgaaatc
     2941 agcgctctac tacctgcccc tacccatgga gcccaaaacc agcaatcatc cgcagttcac
     3001 gctgtttggt aggggacgcc gggtgctttc gagtgcaaaa cttagaataa tgcacgagtc
     3061 ccgttcggtg ttcggcgatc cggtgagccg ttcggggggc atcggctagg agcgtagatg
     3121 cgttggttcg ttgaagtctc tcgcgtcggc gatagtacgc cggccgaacg ctactgtgtg
     3181 gaagcaaagg cgtggcaagc tgcgctgcag gagacgcgca agcttcgcgg agacaccggc
     3241 ccgctttcga agttttcgat cgagctgctc gacgacggtt accgcgccgt cgacccgatt
     3301 cagaagattc gctatctcgt gaaccaagct ccgagcgacg cgcccattgc gttgcccgtg
     3361 aacggcgtgg cgcacgcata gaagccgcct tcgggtgggc actccgcacc tccaccgtcg
     3421 ccgagcgttg ccccgcacgt tggccccagc gtggcgcctg ccccagcggt tcctgccggt
     3481 tctgcagcat cgtctcctcc gctcgcggcg gtcgttggtg ccgcttccgc ggcgccggcg
     3541 ggcgtatcgg ttgctcccac tgcgcccctc gccggcacac cgatcacggc gagcgctccg
     3601 ccagccgtcg caggggcgat tccttcgatt cctgcgctcg cttccggcgg gctcgcagtc
     3661 gctttggcgg ccacggcgcc agctggcgcg aaagccagcg tctccgcagt accgcggccc
     3721 gtttcgcgcc gccctcaaag ccctcacagc gcggacaaga caccgcgacc gccggacgtc
     3781 gcgcccatcg agtccgcagc agcgaagggc agcgcagcac ccgcggccgc ccccgtcgcg
     3841 gtggcgccag ttgcggtgat tgcaccgagt ggtgctccca aagccgctgc atcggcgtcg
     3901 gtgccagcgt ccgccgccgc ttcgtccgcg gccgagctcg cgcgttccgc ggcgccgaaa
     3961 gcgcccgtca aaaccatcgg caacgagtct gccctacgcg cggctacccc ttcggtcgcg
     4021 aaggcaccgc gctcgccggt cagcgctgaa gcgccatcga acgcggtcgc gattcccgag
     4081 catcgggtct tcatgtcgcg ctcgcaagag ccgaagcccg acgcgccgat cacctatcga
     4141 gagcaggcct gcgtggtcgc tcccggtact ccgcgcgaag cggtcgaggc gctgttgcgc
     4201 gagcgctggc aatccgtgaa gaaggaaatc gcgacgcatc catccggtca gctcgtgcaa
     4261 atcgcggtgt tcgatcacgt cttcgagcaa cggccgcagc gttcgccgct cggcacgctg
     4321 gcgtggaaag actggcgggg cgatccggtc ctcggtttcc cggggttcgg cgtgccctcg
     4381 ccgccggtga cgaagagt
//
LOCUS       DASTKS010000420        11551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1182052, whole genome shotgun sequence.
ACCESSION   DASTKS010000420 DASTKS010000000
VERSION     DASTKS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182052"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFQ35_08785"
     CDS             complement(<1..513)
                     /locus_tag="VFQ35_08785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9930769.1"
                     /translation="MPEHSTWLTLLLSYMRDTLEQNAHTLGDSVVGHHPPTWRSFEAL
                     FAGLFVAIVVILIAARTRSRLDRVDEAVIPEDRLTLRTFMETFLSYFYDLAKSVMGPD
                     RAKRYFPLVGTAAMFVFFSNVMALIPGMPVATSHLNITLGCALVVFVFFNAYGLSAQG
                     MGYVKHLAGPV"
     gene            complement(517..906)
                     /locus_tag="VFQ35_08790"
     CDS             complement(517..906)
                     /locus_tag="VFQ35_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930770.1"
                     /translation="MQSDPQNPEDGSIGPVLGSVAIAGALLTIASPALFGRGELASVG
                     AGALLALGNLWVIARVVKSFLGGQGRTAWAPIGFLKLIGLFVVVGLLLKQGVAHVLPL
                     AFGYAALPLGIVLSTLKKPTSPARGEH"
     gene            complement(881..1174)
                     /locus_tag="VFQ35_08795"
     CDS             complement(881..1174)
                     /locus_tag="VFQ35_08795"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET9930771.1"
                     /translation="MQQHWKALGGPGSLGIEIALSVAVGLLGGSWLDKKFGTYPWLTA
                     IGLAYGVAAAGRAVYRALKQANRELEKLDREEREAREKFQREKSQDAERPPKS"
     gene            1299..2252
                     /locus_tag="VFQ35_08800"
     CDS             1299..2252
                     /locus_tag="VFQ35_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930772.1"
                     /translation="MKRAVTCLLVALCASRVASAQSTQAAAPAPPTPNAPAAPAAPRD
                     AAAAPDVPPLPNVPPPPDVPPPPSTTPEASPPPSPGDPPVTEPPLPGPPIFVLEPPPP
                     PVPRHVAPRTSLLLGARVGWFVPFGSLYVEGVRNGNGVAQTRVDIRNYARSGPLVEFD
                     VGARLGRNYGVFGFWERTVFSAGTDTTTFYGHGDQRGGTSDFWGAGVRATSDADHVGF
                     ATELAVGYRRARMEWEDDSAIEATESFPEARFSIGADIRVSKTFSLSPMLSFGVGGFG
                     KVVFVDRNGKATDLIGNEGSPDNHGWFELHVGGHFDVFGRD"
     gene            complement(2341..3807)
                     /locus_tag="VFQ35_08805"
     CDS             complement(2341..3807)
                     /locus_tag="VFQ35_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930773.1"
                     /translation="MSHPRLVGFCLFALCSARAALARADDACTKPFTELVAELECDKY
                     AADRKRRAELSSTDELEAKNKQAIADLDAEIKRLEDDSPLLKKGACAVRTRTCDTLAT
                     TVTMGGDPAALTASLTKLSNATKAEASSADKEVAIRAQEPERQTATNKGGSVAQTDPV
                     ESIQPITLAGGALSLSGTHAGTKGVGNITINPLALAAPGDAIAGRMMDLTISAPFDLE
                     GGTKDSQYVSARLRVNLFAPISASELSREVNAWLQAEGRYADRIEEVLAHAKDVKACA
                     KYVAKTTRVSEEECGQSVDDAQLRAAREKAYEKIASLRRAADKYYLGIDARFDSGDPT
                     GPDVVGDKGTHLLGGLAGGVKIFSSSERWDYELRGRAAGDYFRSRDDSTGTRPKPVYS
                     FDWGAAFILSGKLEDKSKQRLAFGAGVEGRQAGGDQTNARQTPTDFVYLNLMAVVPVV
                     SGGDFGLAFSLPLRERGVERGTIITISSDLGLLDHSTK"
     gene            complement(3804..4160)
                     /locus_tag="VFQ35_08810"
     CDS             complement(3804..4160)
                     /locus_tag="VFQ35_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930774.1"
                     /translation="MPTQVLAPVTEGNSYVVKFTNRVESTGVVLVVVGWVPKHERPVP
                     PPQHMVVAPGATAEIGGKVPPFADARRMVIVASLDPGESGRLVVIENDVERENQRVTE
                     TTTWEALVEEKSEGAG"
     gene            complement(4488..5915)
                     /locus_tag="VFQ35_08815"
     CDS             complement(4488..5915)
                     /locus_tag="VFQ35_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930775.1"
                     /translation="MRSVFIAAVCFIGVGCGSADSIGDATSGSDDSTTNAGNANPAGN
                     NPSDASVGTSNEVVGANESAVTSVTDFCRGRVTDKKTYSLSSLSKPAVGSWVKDPDFP
                     GAQIMRVTNSSSGQATTPMYSTIPAWNADGSLLMLYQWGRGHVLYDGRSFKQIGSLEC
                     QTSSGARPYPSDVEHVMWDPAEPDFVRYPSRYASDSRGALPVLYKCNVRTHVATVEHN
                     FANAPTSCQPGRDELTMGSDPQWYPGGFVGLQCGNNANDTEWHGKKFIYEFKTHKVYT
                     PRSGGKFTGAEAPIVAPSGSRVYFGGAVYDKDLNPMLSLNLASIYEHANVGRSKSGHD
                     LYYAVDFESSNPGIMIGHDMNTGLSIPLISQDGGWGYPGSGIHISSIISDARAAGWAI
                     ASVVGETTRDSYAGEFMLANVDTKAVCRVGRHRSRAGNGGYGYESEPHPVSHVLSDGT
                     LEIAFGSDWGGQSLADTYIMRLKPM"
     gene            complement(6862..8256)
                     /locus_tag="VFQ35_08820"
     CDS             complement(6862..8256)
                     /locus_tag="VFQ35_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930776.1"
                     /translation="MKNVILVSVCLIGVGCGATDSSFTEGNPGFDGEAAARGSDGGGS
                     YKLAGTTSEPASVSDFCSGRVTDKKTHALSTLAKPAVGTWVKDPDFPGAQIMRITNSA
                     KNQATTPMYSTIPAWNADGSLLMLYKWGRGHVLYDGRTFKQIEELTCTTKDGWDPGPS
                     DIEHVMWDPSNPDIVRFPSRYAPDSRGPLPYLYKCNVRTHETSIEHDFSTAPTSCKPG
                     TDELTMGADPQWFPGGLVGLQCGYDVSDTNWRGKKFVYDVNTGKVYTPRSGGKFAKPE
                     APIAAPSGTQVYFDGAVYDKDLNLLRSLNLASTEEHANIGHSVFPHDFYYTVDFEGAD
                     PGVLIAHNMDSGQAIPLISQGLGWGFPGSGIHITSINPDPRAAGWVVTSVIGETTRDP
                     LAGEFLLANVDTKAVCRVGRHRSRAGNGGYGYEAEPHPVSHVLSDGTLEIVFGSDWGG
                     QKLADTYIMRLPPL"
     gene            complement(9544..10191)
                     /locus_tag="VFQ35_08825"
     CDS             complement(9544..10191)
                     /locus_tag="VFQ35_08825"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HET9930777.1"
                     /translation="MGPLLSVVLATQNEGKLSELRQLLADLPIHVLSVRDALGASLDV
                     VEDGATFRDNAVIKARAVCRATHLVSLADDSGLEVDALGGRPGVRSARFAHEHATDAE
                     NNEQLLRELDSVPEARRTARFRCVLALVTPSSDVPSTVDGVCEGSIARAPRGSGGFGY
                     DPLFLVAGEGGRTLAELSEEEKNRISHRGHAMRALRPLLIQILNRKLDEAESVLG"
     gene            10352..>11551
                     /locus_tag="VFQ35_08830"
     CDS             10352..>11551
                     /locus_tag="VFQ35_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9930778.1"
                     /translation="MTTEALVETFHRETDELLTALEQGLLELETTSDAADAIDRLFRA
                     AHTLKGNAGMVGFSEFVRLTHALENVLDRLRAHTLSVSEVVIEASFDAVDALRTSAQM
                     LLDGAPTIVTPEYERALRALAELCEGEPVEAPSLEARLVDAELYFRPEFFANGEDITT
                     FLEELALLGDVIAAETLFDRVPPLDLLAPEACHVGLRARLRTTSGTMGVVGMAVLSVD
                     EENLSVREVPEERKVDAAPANKQDARDPAEPAASVQRSPAHAAHTVAEHIRVDAEKLD
                     GLMDLVGELVVAISQLTTTRSQLVVERLETLGRDLQTRVMSLRMLPVQDAFERFRRPM
                     RDLAKELGKQIEFTTEGGDTQLDKKVIDALLDPLKHMLRNCVSHGLEEPAERERAGKP
                     ASGRVHVS"
BASE COUNT         2042 a         3818 c         3637 g         2054 t
ORIGIN      
        1 cacgggcccc gcgaggtgct tcacgtagcc catgccctgc gcagacaggc cgtaggcatt
       61 gaagaacacg aacacgacga gcgcgcagcc gagcgtgatg ttcagatggc tcgtcgcgac
      121 gggcatgccc gggatgagcg ccatcacgtt cgagaagaag acgaacatgg ccgcggtgcc
      181 gacgagcggg aagtaacgct tcgcccgatc cgggcccatc acgctcttcg cgagatcgta
      241 gaagtaggag aggaacgtct ccatgaacgt gcggagcgtg agacgatctt ccggaatcac
      301 cgcctcatcg acgcgatcga gccgcgaacg cgtacgcgcc gcgatgagga tcacgacgat
      361 cgcgacgaac agccccgcga agagagcctc gaagctacgc cacgtcggag ggtgatggcc
      421 cacgacgctg tcgccgaggg tatgcgcgtt ctgctccagt gtgtcgcgca tgtaagagag
      481 caagagagta agccacgttg aatgctcggg catgtttcag tgttctccgc gtgcgggcga
      541 agtgggcttc ttcagcgtcg acaacacgat gccgagcggc agcgctgcgt aaccgaaggc
      601 gagcggcagc acgtgagcga cgccctgctt caacagcaag ccgacgacca cgaacaggcc
      661 aatcaatttc aggaaaccaa tcggggccca ggctgtgcgc ccttgaccac cgagaaaact
      721 ctttacgacg cgcgcgatga cccagaggtt tccgagcgcg agcaacgcgc cggcgccgac
      781 gctagcgagc tcaccgcgac cgaatagagc cggagaagcg atcgtcagca acgcgcccgc
      841 aatggcgacc gaacccaaca cgggtccgat gcttccgtct tcaggatttt gggggtcgct
      901 ctgcatcttg ggacttctcg cgttggaact tctcgcgagc ttctcgttct tctcggtcta
      961 gtttttcgag ctcacggttg gcttgcttga gcgcgcgata gacggctctg cccgcggctg
     1021 caacaccgta tgcaaggccg attgcggtga gccaggggta cgtgccgaac ttcttgtcga
     1081 gccaggatcc cccgaggagc cctacggcca cggatagcgc tatctcgata ccgagcgagc
     1141 ctgggccgcc aagagctttc caatgttgct gcacattcct cgcgggggcg gcgcggaatt
     1201 agcacgcagt acggctcgcg gtcaatgcga cggctgagcg ttgacagcac tccggcacga
     1261 tccctcgaac gtggcgccga tttgctatgg ttcacgcgat gaaacgagcg gtaacgtgcc
     1321 tattggtcgc gctctgcgcg agccgggtcg ctagcgcgca atcgacgcaa gccgctgcgc
     1381 ctgctccgcc gactccgaac gcacctgctg cccccgctgc accgagagac gccgcggcgg
     1441 cgcccgacgt tccgcctctg cccaacgttc cacctccgcc ggacgttccg cctccgcctt
     1501 cgacgacgcc ggaggcgtca ccaccaccga gtcccggcga cccaccggtc acggagcctc
     1561 cgctgcccgg ccctcccatt ttcgtcctcg agccgcctcc gccacccgta ccgcggcacg
     1621 tggcaccaag gacgtctctc ttgctcggcg cgcgcgtcgg ctggttcgtg ccgttcggta
     1681 gcctgtacgt cgaaggcgtt cgtaacggaa acggcgtcgc gcagacgcga gtcgatatcc
     1741 gcaactatgc tcgctcggga ccactcgtgg agttcgacgt aggtgcccgc ttggggcgca
     1801 actacggcgt attcggcttt tgggagcgca ccgtgttcag cgccggcacc gacacgacga
     1861 cgttctacgg gcacggagat cagcgcggcg gcaccagcga cttctggggc gccggcgtgc
     1921 gtgcgacttc agatgcggat cacgtcggct ttgccacgga gctcgcggtg ggttaccggc
     1981 gcgcgcggat ggaatgggag gacgacagcg cgatcgaagc gactgagtcg ttcccggagg
     2041 cgcgcttctc catcggtgcc gacatccgag tgtcgaagac gttctcgctc tcacccatgc
     2101 tgagcttcgg cgtcggcggc ttcggcaagg tcgtgttcgt cgacaggaat ggaaaggcaa
     2161 ccgacctcat cggcaacgag ggcagccccg acaaccatgg ctggttcgag ctgcacgtgg
     2221 gcgggcactt cgacgtcttc gggcgcgatt agcgaagaac gacgcgagcc cgccgcgtct
     2281 gtctcgagag agaggcgggc gggctcgact gccgtcggcg gtgcttgcgc ccggcggact
     2341 tcacttggtc gaatgatcga ggaggccgag gtccgatgaa atcgtgatga tggtccctcg
     2401 ctcgactccc ctttcgcgca gcggcaggct gaacgcgaga ccgaagtctc ctccgctcac
     2461 gacgggaacg accgccatca agttcaagta aacgaagtcc gtcggtgtct gtcgagcgtt
     2521 cgtctggtcg ccaccagctt ggcgcccttc cacgccagcg ccgaacgcca agcgctgctt
     2581 ggacttgtct tcgagcttgc cagatagaat gaacgcggcg ccccaatcga acgagtacac
     2641 cggtttaggt cgcgttccgg tcgagtcgtc gcgagaacga aagtagtcac cggcggcgcg
     2701 cccgcgcagt tcgtagtccc agcgctcgct tgacgagaag atcttcaccc cgccggcgag
     2761 accgccgagc agatgagttc ctttgtcacc gaccacgtcc ggcccggtgg ggtcgccgga
     2821 gtcgaagcgc gcgtcgatgc cgaggtagta tttgtccgcc gcgcgccgaa ggctcgcgat
     2881 cttttcgtac gctttttcgc gagccgcccg cagttgcgcg tcatcgacgc tttgcccgca
     2941 ttcttcctcg gagacgcggg tcgtcttggc cacgtacttg gcgcacgctt tcacgtcctt
     3001 cgcgtgcgcg aggacctctt cgattcgatc cgcgtagcga ccttcggctt gcagccaagc
     3061 attgacctcg cgggagagtt cgctcgcgct gattggagca aagaggttga cgcgcaaacg
     3121 cgccgaaacg tactgactgt ccttcgttcc gccctcgagg tcgaagggcg cgctgatcgt
     3181 caggtccatc atgcgcccgg caatcgcgtc gcccggcgcg gcgagcgcga gggggttgat
     3241 cgtgatgttg ccgacgccct tcgtgccggc gtgagtgccc gagagggaga gcgcgccgcc
     3301 cgccagcgtg atcggttgaa tcgactcgac ggggtccgtt tgcgcgaccg agccaccttt
     3361 gttggtagca gtctgccgct ccggctcttg ggcgcgaatc gccacctctt tgtcggcact
     3421 gctcgcctcc gctttcgttg cgttgctcaa tttcgtcagc gaagcggtga gcgcggccgg
     3481 atccccaccc atggtcacgg tcgtcgcgag ggtgtcgcac gtcctcgtcc gaacggcgca
     3541 cgcgcctttc ttcaagagcg gactatcgtc ctccaggcgt ttgatctcgg cgtcgaggtc
     3601 ggcgatggct tgcttgttct tcgcttcgag ctcgtcggta ctcgaaagct ccgctcggcg
     3661 cttgcggtcc gccgcgtact tgtcgcattc gagctcggcg acgagctcgg taaagggctt
     3721 ggtgcaagcg tcgtcagcgc gggcgagcgc cgcgcgcgcc gagcacaacg caaagaggca
     3781 aaacccaacc aggcgtggat ggctcatccc gcaccctccg acttctcctc gaccagcgcc
     3841 tcccaagtcg tcgtttcggt cactcgctgg ttctcgcgct cgacgtcgtt ttcgatcaca
     3901 acgagcctgc ccgactcgcc tgggtcgagc gacgcgacga tgaccatgcg ccgcgcgtcc
     3961 gcgaatggag gcaccttgcc gccaatctcg gcggttgcgc cgggcgcgac gaccatgtgc
     4021 tgcgggggtg gcaccggcct ctcgtgcttg ggcacccacc cgaccacgac gagcaccacg
     4081 cccgtgctct ccactcgatt cgtgaacttg acaacgtagc tattgccctc ggtcaccgga
     4141 gccaagacct gcgtcggcat cagtcgttcc ctccacttgc ttcaactcgg accatcgaac
     4201 cccacatttc gcccgactcc cccgatcctg cctatggtca tttgtgcggt gcattggggg
     4261 atcgagagac ggaccgacct cgggggttac ggacccaagc cagtccgata gcccggatgg
     4321 gagccggtta aaaccggctc atcgctacgc ccgcgtcgga gccccaaaag cgaacgcccc
     4381 gggaaaactc ccgaggcgtt tcgctgaccg ggcgctcccg gtgctccact tacacggccg
     4441 ctagctacgc ccgagcgatc cgtcgagcga gccgcgccga tccctcctca catcggcttc
     4501 aaccgcatga tgtaggtgtc ggccaagctc tggccacccc agtcgctgcc gaacgcgatt
     4561 tcgagcgtgc cgtccgacag gacgtgagag acgggatgcg gctcggattc gtagccgtac
     4621 ccgccgttgc cagcgcgact gcggtgacgg ccgacgcgac acaccgcctt ggtgtcgacg
     4681 ttcgcgagca tgaactcgcc cgcgtacgag tcgcgcgtcg tctctccgac gacggacgcg
     4741 atggcccagc cagccgcgcg cgcatcggaa atgatcgagc tgatgtggat gccgctacca
     4801 ggataacccc aaccgccgtc ctgagagatg agcgggatcg atagccccgt gttcatgtcg
     4861 tgcccgatca tgattccggg gttcgagctt tcgaagtcga ccgcgtagta taggtcgtgc
     4921 cccgacttcg aacgaccgac gttggcgtgc tcgtagatgc tcgccaggtt caaggagagc
     4981 atcgggttga ggtccttgtc gtagacggct ccaccgaagt acacgcggct tccgctcggc
     5041 gcgacgattg gcgcttctgc tccagtgaac ttgcctccgg agcgcggcgt gtagaccttg
     5101 tgggtcttga actcgtagat gaacttcttg ccgtgccact cggtgtcgtt cgcgttgttg
     5161 ccgcattgca gaccgacgaa accgcccggg taccactgcg gatcggaacc catcgtgagc
     5221 tcgtcgcgac caggctgaca agaagtgggc gcgttcgcga agttgtgctc gacggtggcg
     5281 acgtgcgtgc gcacgttgca cttgtagagc acgggcaggg caccgcgaga gtcggacgca
     5341 tagcgcgacg ggtaccgcac gaaatcgggc tcggcaggat cccacatcac gtgttcgacg
     5401 tcgctcgggt atgggcgcgc cccgctgctg gtctggcact cgagcgagcc aatctgcttg
     5461 aaggaccgac cgtcgtagag cacgtggccc ctgccccact ggtagagcat gagcaacgag
     5521 ccgtccgcgt tccacgccgg gatcgtcgaa tacatcggag tggtcgcttg gccactgctc
     5581 gagttcgtta ctcgcatgat ctgcgcgccg gggaagtctg gatccttcac ccaggagccg
     5641 actgccggct tggatagtga gctcagcgag tacgtcttct tgtcggtgac gcgaccacgg
     5701 caaaagtcgg tcacgctggt aacggcgctt tcattggcgc ccacgacctc gttcgacgta
     5761 ccgacgctcg cgtcgctcgg gttattgccc gcgggattcg cgttaccggc gttggttgtc
     5821 gaatcgtccg acccgctcgt cgcgtccccg atggaatcgg ccgaaccgca cccgacaccg
     5881 ataaaacaaa ccgctgcaat gaacacactt ctcatctaga cctcgatggt tgctggtttc
     5941 agataccggc ccacgcgaag atgcaccggc tagaggataa gcgctctcag gctattttct
     6001 cgaagaatgg ccgagcgcga cgaacttcgg atcttcgccg cgcgcgccca ccgaactcgg
     6061 caaggacggt gagggcgcaa ggtgtagcaa acgatttcga gatgccaacc ccgcactgag
     6121 aataacagcg cgaggaatgt tcaaatatct attctgttgc gaaccaaccc agacgacaga
     6181 gcgacacact cgcacgagag tgacactcaa cgagccgctc tcggcgactg cgctcgcgcg
     6241 ctcacccaaa ttcgttggtg cgcggcgctg ggtgggccgc actaaaacac aaacggagat
     6301 tgtgcgcgcg acttcagggg cagcgagtcg cactttctct gcgacgcgcc gaccacgagc
     6361 gcgagcagcc tactccccgg agatatcgaa ccgcgcgaag aacgtgttcg agcgtacgtt
     6421 tggcccgtcc gagttggggc gcatgcgcct cagcgcgacg aaacagtgcg ccccagcgcg
     6481 caattcgaga gtggcgtttt gcaccagcgc gccgcgatac gcctcagtct gagcgacacc
     6541 gcacatggca ttttttcttt cgaatttgcg catcgcaatc agcgcgcgcg tcgcgaactc
     6601 actccgacga gcgactggtc tcgacgctca ttgcggggag aaccaaaggt cacgaagtga
     6661 ctgtacttcc gatgtagatc ggacgcctcg cacgccgaac accgatccat gctcgacgga
     6721 tattttcgga gtcgagctgc acatcgtgca acgcggttgc ccgtcgcagc gcgcgcgtcg
     6781 caaccgcgga gcagcccgac tccaaacatt acgctgagta gtccccggcg cggggctcgg
     6841 gtggcatgtc cctacagctc ttcacaacgg gggcagccgc atgatgtacg tgtcggcgag
     6901 cttttgtccg ccccaatcac tgccgaacac gatctcgagc gtgccgtccg ataagacgtg
     6961 cgagaccgga tgcggctccg cttcgtagcc gtagccgccg ttgccggcgc ggctccggtg
     7021 acggccgacc cgacacacgg ctttcgtgtc gacgttcgcg agcaggaact cgcccgcgag
     7081 cggatcgcgc gtggtctccc cgatgaccga ggtgacgacc cagccggccg cgcgcggatc
     7141 cgggttgatg gaggtgatgt ggatcccgct gccggggaac ccccacccga gaccctgcga
     7201 aatgagcgga atggcttggc cagagtccat gttgtgagcg attaagacgc cgggatcggc
     7261 gccctcgaag tcgaccgtgt agtagaagtc gtgcgggaaa accgaatgac cgatgttcgc
     7321 gtgctcctcg gtgcttgcga ggttgagcga acggagcaga ttgaggtcct tgtcgtagac
     7381 ggccccatcg aagtacacct gcgttccgct cggcgccgcg atgggcgctt ctggctttgc
     7441 gaacttgccg ccggaccgcg gcgtgtacac cttgccggtg ttgacgtcgt agacgaactt
     7501 cttcccgcgc cagttcgtgt cgctgacgtc gtatccgcac tgcaacccca cgagccctcc
     7561 cgggaaccac tgcgggtcag cgcccatcgt gagctcgtcc gtgcctggct tgcaggacgt
     7621 cggcgcggtc gagaagtcgt gttcgatcga ggtctcgtgc gtgcggacgt tgcacttgta
     7681 gaggtagggc aagggcccgc gcgaatccgg cgcgtagcgc gacgggaacc gcacgatgtc
     7741 ggggttggaa ggatcccaca tcacgtgttc gatgtcgctc ggaccgggat cccagccgtc
     7801 tttggtcgtg caagtgagct cttcgatctg cttgaacgtg cgaccatcgt agagcacatg
     7861 acctcttccc cacttgtaga gcatgagcag cgagccgtcc gcgttccagg cggggatggt
     7921 cgagtacatc ggagtcgtgg cttggttctt ggccgagttc gtgatccgca tgatttgagc
     7981 gcccggaaaa tccgggtcct tcacccacgt gccgaccgcg ggcttggcca gcgtgctcag
     8041 tgcatgcgtc tttttgtcgg tcacgcgccc gctgcagaag tcgctcacgc tcgcgggttc
     8101 gctggtcgtc cccgccaact tgtaggagcc gccgccgtcg cttccgcgcg ccgccgcttc
     8161 accgtcaaac ccggggttcc cttcggtgaa cgaagagtca gtcgcaccgc agccgacgcc
     8221 gataagacac accgatacta ggatcacgtt tttcatcgag acctctcctg gtttgcaggt
     8281 ttcagacgcc ggtacagcgc gaagacgtac cggtttttca gcaatcaccc tcgcgggtta
     8341 tgcacgacat tccactcggg cgacccacag gcgtcactcg cgcttcttcg ccgtctcgcc
     8401 gagctggccg gagccgcaag gcagagcacg attgggaagg ccgaccctat gtcgccagta
     8461 acaaaacgaa gcggttcgga tttccgcgcg aagctacacc cgccgcaatt cggcgagcgt
     8521 gatgactaat gacctccaac tcgcggggtt cgcccgcggt tcttgacgtt ctcctccctc
     8581 atgggtcgcc gaatgcggcg ccgcgtggcc cgcatcagag cgcgactcga gatcttgcgc
     8641 gtgcccgcgg agcgagcgac tcgcgcctga cgcgcgacat cgaccccacc cgcggcgtcg
     8701 ctcgcaccgc tgctcgtcga acgctgcgtg gcgcgaccgc agcgctcgac tggccgcgaa
     8761 acacgccaac tccctgcagc gccgtcacat cgtgtcgaac gagcccgccc cacgggaacc
     8821 ccgagaaacc gcgctgcgcg cggcccatcg gaagagccgg ggatgcaacc acgcgagagc
     8881 gagcaggagc tcaccgcggc gtcgacgcac ccgaaagaca gagatccgat gagttggcct
     8941 tcgctccagc ggtgaggcga acatgccttg tgccgcacat tcgtcgacgc gttgcgaagc
     9001 ccttcaatcg catttccttc tgattgggtg ggacgcacgt cgctcgcaag ttgcgaaatg
     9061 tgtgcccacc gcaaccgccg tttgatcgag cgctcgccgc gccggatcga gccgtttgac
     9121 gcgcgccgcg cgccgcgttt gagcgaatcc tttctcgcac actgaggcag tcgagcacgc
     9181 tggtggcgcg cgcctcggac ccgcagatcg ccgtgtcgta gcgcgcaact ggccgcgtcg
     9241 tagcgcgcaa ctggccgctg gctgaacacc gagaaaaatg cgcttcgttg ctccaccaac
     9301 ccgcgcgcaa agcgggcaac gcgagccaat tcgagacagc gcatcgttgg ctaccgcctc
     9361 gctccggagc gccccagcca atctgttaca gcgattctcg cggatgcgag tcgctcacga
     9421 gcgccaactc accccatggg ggcagacgcg cagcgcctcc cccgcggggt agcagcgacc
     9481 accagtgggg gcgcgatttt tccgtccccc gcctcgtcgg ccgcgcccac gccggactgc
     9541 cgctcagccg agcaccgact cggcttcgtc gagcttgcga ttcaatatct gaatcaacaa
     9601 cgggcgcagg gcgcgcatcg cgtggccgcg gtgactgatg cggttcttct cctcttcgct
     9661 gagctcggcc agcgtgcgcc cgccttcgcc cgcgactaga aacagcggat cgtagccgaa
     9721 gcctccgctc ccgcgcggcg cgcgcgcaat gctgccctcg cacacgccgt cgaccgtgct
     9781 cgggacatcg ctcgacgggg ttaccagcgc gagcacgcaa cgaaagcgcg ccgtgcgccg
     9841 tgcttcgggg acgctgtcga gctcgcgcaa tagctgttcg ttgttctcgg cgtcggtcgc
     9901 gtgctcgtga gcgaagcgcg ccgagcgcac accggggcga ccacctagag cgtcgacctc
     9961 gagcccgctg tcgtcggcga gggagaccag gtgcgtcgcg cggcacacgg cacgtgcctt
    10021 gatgacggca ttgtcgcgga aggtcgcacc atcctcgacc acgtcgagac tcgcgccgag
    10081 cgcatctcgt acactcagaa cgtggatcgg caaatccgcg agtaactggc gtagctcgct
    10141 cagttttcct tcgttttgag tagccaatac gaccgagagc aagggtccca ttgccgcgac
    10201 tatatagcgt cgagggcaat tggggagtcg ccacacgcaa gaattgcggc tgaggcgtgc
    10261 accgcttgcg cccgtaaaat ctcagctacg cgcgcgaaac ggccgcaaaa tggcttcagt
    10321 cagtccgggg acttccgttc gtcactcgag aatgaccacc gaagctctcg tggaaacttt
    10381 ccaccgagaa actgacgaac tcctgacggc gctcgaacaa ggcctgctcg agctcgagac
    10441 tacctcggac gcggcggacg caatcgaccg cctatttcgc gccgctcata cgctgaaagg
    10501 caacgcgggc atggtggggt tcagcgagtt cgtgcgcctg acccacgcgc tcgagaacgt
    10561 gctcgatcgc cttcgcgcgc acacgctgtc ggtgtccgag gtcgtcatcg aggcgagctt
    10621 cgatgccgtc gacgcgctga ggacgtccgc gcagatgctc ctcgacggcg cgccgaccat
    10681 cgtcacacca gagtacgagc gggcccttcg agcgctcgcc gagctgtgcg agggcgagcc
    10741 cgtcgaagcc ccgtctctcg aggcacgcct agtcgacgcc gagctctatt tccggcccga
    10801 gttcttcgcc aacggcgagg acatcacgac ttttctggag gagctcgcgc tgctcggcga
    10861 cgtcatcgcg gccgagacgc tcttcgaccg ggtcccgcct ctcgacctac tcgcgcccga
    10921 ggcctgtcat gtgggcctcc gcgcgcgcct gcgcacgaca tccggcacca tgggcgtggt
    10981 cgggatggcg gtgctgtcgg tagacgaaga gaacctcagc gtgcgcgagg tccccgaaga
    11041 gcgaaaggtg gacgcagcgc ccgcgaacaa gcaagacgcc cgcgatccag ccgagccggc
    11101 cgcatccgtc cagcgctcgc cagctcacgc tgcgcacacc gtcgccgaac acatccgcgt
    11161 cgacgcggag aagctcgatg ggctgatgga cctcgtggga gagctcgtcg ttgccatttc
    11221 gcagctgacg actacccgat cccaactcgt cgtggagcgg ctcgaaacac tcggccgcga
    11281 cctgcagacg cgcgtgatga gcttgcgcat gttgccggtg caggatgcct tcgagcgctt
    11341 ccggcggccg atgcgcgatc tcgccaagga actcgggaag cagatcgagt tcactaccga
    11401 gggcggcgac acccagctcg acaagaaggt gatcgacgcg ctgttggacc cgttgaagca
    11461 catgctgcgc aactgcgtgt ctcacggact cgaggagccg gcggagcgcg agcgcgccgg
    11521 gaagcccgcg tccggtcggg tgcacgtgtc g
//
LOCUS       DASTKS010000421         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_513479, whole genome shotgun sequence.
ACCESSION   DASTKS010000421 DASTKS010000000
VERSION     DASTKS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513479"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFQ35_08835"
     CDS             <1..713
                     /locus_tag="VFQ35_08835"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9930779.1"
                     /translation="SELSFAKDARLAKIYGVGAWDGSSAPPALPSGQRPGLLTRALFL
                     TTGTANTRPILKGVFIRKNVLCNEIPPPPPGANAKPPTLGPNMTTRESVEELTEMSGT
                     TCAGCHATLINPLGYATEGFDSLGRFRTEQRLFDDSGAQTGSKPVDTSSIPRVTLGDM
                     TPIRGAGELMSLIASSGQVEACMSRNFFRFTYGRWEDTAFDGCALEDGRKALSNGGRL
                     SDLITAELMSPAFRRRAF"
     gene            724..2301
                     /locus_tag="VFQ35_08840"
     CDS             724..2301
                     /locus_tag="VFQ35_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9930780.1"
                     /translation="MDLTKRVIDVSRRQVLRGLGATVALPILPSLLTKSAYGADPVFT
                     RAPRLYWVTTDHGGAFEASMFPSQSLLTASAAAFSDHAVRSGALAAAAQGDSFGVSTV
                     LRAGSSAFTPALLAKMNVLHGLDVPFYIAHNTGLHLGNYARNDGNGNDGKAVQQFPRP
                     TIDQIMAWSPSFYPDLSSIKERALVMGDRPVSWNYTDPKAPDSSSISNVRGNSSSLDL
                     FKRIFVAPTSSEPAPRAPIVDRVLANYKRLRNGNTRLSAGDRQRLDDHIARIAELERK
                     LTAKVGSSSASCSAVTKPTDDANKHNGNSPSEAGRQLELFNEVAAVAFMCGTSRIAVL
                     AYGDTSRFVSYGGDWHQEVAHQWQLAEAQDALVQSYQRFFESVVVDMANRLNVEESPG
                     VTFLDNSLVVWTQESGMETHGSVSVPVVTFGSAAGSLKTGLYCDYRRIGNKGSEYDPG
                     AGGKQTLGLLYNQWLATVLQAMRVPASEFERWGYKGYGYPYITKESWTPPFAKHYGDT
                     SSRYFQMASDYLPFLKA"
     gene            complement(2347..>3335)
                     /locus_tag="VFQ35_08845"
     CDS             complement(2347..>3335)
                     /locus_tag="VFQ35_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HET9930781.1"
                     /translation="AGGAAGRGGTSAGGASVGGASVGGASVGGASVGGASVGGASVGG
                     RGGASSCSGTPTSYDELVLCDSPVGYWAMSQASGSEPDLSGRGNVGSYRGGSAGTTTL
                     PNGDRAAVFNGSNQFLTIPSLPAYSIPTTGSLTWEAWLKASVLQFPHDSGGYVDWLGK
                     CASYSPTCEWEGRLYNTDNSESRCNRFSAYVFNPSAGLGSGADFQPQCGLIQAGNWYH
                     VVGEYTLLSAPASCENTSKYPGSIDIWVNGVKWNQSRHGQTGCMSQYQVVPKANSSPI
                     NVGTMAEDAWFQGAIAKLAIYDHLLSEAQIKARYRAMTAREPTGSCANDCSF"
BASE COUNT          620 a         1125 c         1030 g          560 t
ORIGIN      
        1 cgagcgagct ctctttcgca aaggacgcac ggctcgccaa gatctatgga gtcggcgcgt
       61 gggatggaag ctccgctccg cccgcgctgc cgtcaggaca gcgaccgggg ctcctgactc
      121 gagcgctatt cctcacgacc ggcacggcca acacgcgtcc catcttgaag ggcgtcttca
      181 ttcgcaagaa cgtgctgtgc aacgagatcc cgccgccccc tccgggcgcg aacgcgaagc
      241 cgccgacgct cgggcccaac atgacgaccc gcgagagcgt cgaggagctc accgaaatgt
      301 cgggcactac ctgcgccggc tgccacgcga cgctgatcaa tccgctcggc tacgcgacgg
      361 aaggcttcga ctcgctcggt cgtttcagaa cggagcagcg cctcttcgac gacagcggcg
      421 cgcaaacggg cagcaaacca gtcgacacat cctccatccc tcgcgtgacg ctcggcgaca
      481 tgacacccat tcgaggcgct ggcgagctca tgagcctgat cgcttcgagc ggacaagtcg
      541 aggcgtgcat gtcacgaaac ttcttccgct tcacgtatgg gcgctgggaa gacaccgcgt
      601 tcgatggttg cgcgttggag gacggccgca aggcgctgag caacggcgga aggctgagcg
      661 acttgatcac ggctgagttg atgtcacccg cgttccgccg gcgtgcattt tgagagagcg
      721 aggatggatt tgacgaagcg agtcatcgac gtttcgaggc gacaagtgct ccgcgggctc
      781 ggcgccaccg tggccctacc gattctgcct tcgcttttga cgaagagcgc gtacggcgcc
      841 gaccccgttt tcactcgcgc gccgcggttg tactgggtga ccacggacca cgggggcgca
      901 ttcgaagcga gcatgttccc gagccaatcg ctcctcacgg cgagcgcggc ggcattttcg
      961 gatcacgccg tacgttccgg cgcactcgcg gcggcagcgc aaggcgactc gttcggcgtt
     1021 tcgacggtac tgcgggcggg ctcttccgcg ttcaccccgg cgctgctcgc caagatgaac
     1081 gtgctgcacg ggctcgatgt ccccttctac atcgcgcaca atacggggct tcacctcggc
     1141 aactacgcgc gcaacgacgg caatggcaac gacggcaagg ccgtccagca gttcccccgc
     1201 cccaccatcg accaaatcat ggcttggtcg ccgtcgttct accccgacct ttccagcatc
     1261 aaggagcgcg cgctcgtgat gggcgatcgc ccggtttcgt ggaactacac ggacccgaag
     1321 gcgcccgatt cgagctccat cagcaacgtg cgcggcaact cgagctcgct cgacttgttc
     1381 aaacgaattt tcgttgcgcc tacgagctcg gagccggctc cgcgcgcgcc gatcgtcgat
     1441 cgcgtgctcg cgaactacaa gcgcctgcgc aacgggaata cccggctctc cgccggcgac
     1501 cgtcaacggc tcgacgacca catcgcgcgc atcgccgagc tcgaacgcaa gctcacggca
     1561 aaggtgggct cgagcagcgc gtcgtgctcg gccgtgacca agccgaccga cgacgccaac
     1621 aagcacaacg gcaactcgcc gagcgaagcc ggacggcaac tcgagctgtt caacgaggtc
     1681 gcggcggtgg cgttcatgtg cggcacgagc cgcatcgccg tgctcgctta cggcgacact
     1741 tcgcgcttcg tctcctacgg cggggattgg caccaagagg tcgcgcacca gtggcagctc
     1801 gccgaagcgc aggacgcgct cgtccagtcg taccagcgct tcttcgagag cgtcgtggtc
     1861 gacatggcga accgcctgaa cgtcgaagag agccctggcg tcacgttcct cgacaattca
     1921 ctcgtcgtct ggacgcaaga gagcgggatg gagactcatg ggtcggtgtc ggtgccggtc
     1981 gtcacgttcg gtagcgcggc cggaagcttg aaaaccggcc tctactgcga ctaccggcgc
     2041 atcggcaaca agggctcgga gtacgacccc ggcgcgggcg ggaagcaaac tttggggctc
     2101 ctctacaacc aatggctcgc gacggtgctc caggccatgc gcgtaccagc gagcgagttc
     2161 gagcgctggg gctacaaggg ctacggctac ccctacatca ccaaagagag ctggactccg
     2221 ccgtttgcaa agcactacgg cgacacgagc tcacgatact tccaaatggc gagcgactac
     2281 ttgcccttct tgaaggcgtg acgaccgcga gtccgacgcc gcggagacca cagcctagac
     2341 cgccgatcaa aacgaacagt cgtttgcgca gctgcccgta ggttccctcg ccgtcatcgc
     2401 gcggtagcga gctttgatct gcgcttcgct gagcaggtgg tcgtaaatgg caagctttgc
     2461 gatcgcccct tggaaccacg cgtcctcggc catggtgccg acattgatcg ggctcgagtt
     2521 tgccttgggc acgacttggt attggctcat gcagccggtc tgcccgtggc gcgactggtt
     2581 ccacttgacg ccgtttaccc aaatgtcgat cgacccgggg tacttgctcg tgttctcgca
     2641 actcgcgggt gcggacagca acgtatactc gccaactacg tggtaccaat tgcccgcctg
     2701 aatcaagccg cactgaggct gaaagtcagc cccagaaccg agccccgcgc tcgggttgaa
     2761 tacgtaggca gagaagcggt tgcagcggct ctccgagtta tcggtgttgt agaggcgccc
     2821 ctcccattcg caggtcggcg agtaggacgc gcacttgccg agccaatcga catagccgcc
     2881 gctgtcgtgc ggaaactgaa gaacactcgc cttgagccaa gcttcccaag tcaggctccc
     2941 ggtggtgggg atggagtatg cgggaagcga cggaatcgtc agaaactggt tcgagccgtt
     3001 gaacacggca gcgcggtcgc cgtttggaag cgtcgtcgtt cctgcgctcc cgccccggta
     3061 gctgccaacg ttgccgcgac cgctgagatc gggctcgctg ccggaggcct gcgacatcgc
     3121 ccaatatcca accggcgagt cgcacaacac gagctcgtcg tagctagtcg gtgtcccgct
     3181 gcacgatgag gcaccgccgc gaccgccaac gctcgcgccg ccaacgctcg caccgccaac
     3241 gctcgcaccg ccaacgctcg caccgccaac gctcgcgccg ccaacgctcg cgccgccagc
     3301 gctggtgccg cctcgacccg ctgcgccgcc ggcaa
//
LOCUS       DASTKS010000422         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1182135, whole genome shotgun sequence.
ACCESSION   DASTKS010000422 DASTKS010000000
VERSION     DASTKS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182135"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFQ35_08850"
     CDS             <1..540
                     /locus_tag="VFQ35_08850"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9930782.1"
                     /translation="VGETRVARSTATSQELPPCLSQRIAQARFGRPGRARVARWTFVG
                     GLVRFEDAETRRRNRLNQRRKNRDANDGETGIVIERESPGKLDFDTVEGIVHDGYRLF
                     AHCYRDGLARDSTLGGAVRLRFVIGSNGRVARVIDGGSDLPDEGVLACVAEGFFALQF
                     PKPTGGNVHLRYRMHFDSG"
     gene            1609..>3395
                     /locus_tag="VFQ35_08855"
     CDS             1609..>3395
                     /locus_tag="VFQ35_08855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930783.1"
                     /translation="MNPKTLEGQLTAGQMLGRYEMLLPIAAGGMGNVWAARLKGTRGF
                     RKLVAVKTVLRAINDEKLEQMLFQEATLASQIHHPNVVETIELGEHDGTMYLVMELVY
                     GESLSFILREAVARGGVPLTVAVNLVGQVCRGLQAAHDLTDAYGNPVGLVHRDISPSN
                     VLVTYSGNVKIVDFGVATTSTTSQSSSPEIKGKLSYLAPEQLRAGEVDARVDVYATGL
                     LLYLMTVQKHPFRGASEGETINRILSEQPVTPPSAFVEDYPEGLEAVLLKALEKNREN
                     RYGSAASLLEALESALPEAFGPNGDRQTVEYLQELLKPRMEERRATLRMAEDLAEQSS
                     ARDSALSLPAMVSPPPLPSQPSSRANRFAVGAALVAACALALGAKGIFLKGPTQHHAS
                     AAIAAAVAGDPVAPQSLPAPAAPAAPTSLVASPATPEAPASAAIAEERAAPSLASAPH
                     LVAHRGARGERSEHGAGPAVLSDVAAYDAGAAAAPSASATPEADAAAALQVGSGLALA
                     PSAEVTAQSAQPTTVNPPPAALAPSAPVATAERDLVPRRVGSRIGNARLAVNPGADGY
                     RARIPRALERTQQSFRATVDICVNTDGKV"
BASE COUNT          641 a         1114 c         1078 g          562 t
ORIGIN      
        1 gtcggcgaga cgcgggtagc ccggtcgacc gcgaccagcc aagagctccc tccctgcctt
       61 tctcagcgca tcgcgcaggc tcgcttcggc cgccccggac gcgcccgcgt cgcccgctgg
      121 accttcgttg gaggactcgt ccgcttcgag gacgcagaga ctcgccgtcg taaccgcctc
      181 aaccagagac gcaagaacag ggacgcgaac gacggcgaaa cgggaatcgt catcgagcgc
      241 gaatccccgg ggaaactgga cttcgatacc gtcgagggca tcgtccacga tggctaccgc
      301 ctctttgcgc actgctatcg agacggcctc gcccgggatt cgaccttggg cggggccgtg
      361 cggctccgct tcgtaatcgg ctccaacggg cgcgtggctc gagtcatcga tggagggtcc
      421 gacctaccgg acgagggcgt cctcgcctgc gtcgccgagg ggttcttcgc cctgcagttc
      481 cccaagccga ctggcggaaa cgtgcacctc cgctaccgca tgcatttcga ttcgggctga
      541 tcaccgcaac gaatcaccca cgttcaggcg gttcgacacg ttaagaaagt gaccagggcg
      601 tccccaagtc tctgcccgtg acgagcaccg agaaacgcga ccgagaaccg cgccgaggag
      661 tgagggctgg agaaaggccg acgccttccg ggatcgaagc gatgacgtca cgcttcgaaa
      721 ccgcaggatc ttggttcgca cggcggagaa cttggggaca gaccgagccg cgggcggtcg
      781 atgctcggac tatcggcaac ccgatggcgc ttcggcgcgg aggctcgtcg tgtcgccgaa
      841 gcccagccgc gcgacgaagt cggcagtcga aatcgcgaat ccggcgcgtg ttctgaccga
      901 caaagcaacg tgattagtcg aggactagta cgaaagcgta acgcactggg ccgtaccttc
      961 gacgctacgc gtcctacctc tcgcccctgc gtcaaacccc cgaattgccg agagataccc
     1021 acatcgcgcg tcatgtaggc ggatttttcg gctccgcaaa tacgcgtctc cattcgtggg
     1081 gatgaagcat ttggggggat gactctggcc gccgcgaccc aataaacaag aagacacaac
     1141 ttcaacgggt tacccggaac caggatgtag ttttgcgcta ccctggtgta ttacaaacga
     1201 gtcccgcggg cagaccatgt gggtaggaac atttcgtcgc atatcacgga gcgagagcgc
     1261 gagttcgcgt gcgccgaggc ttaccgaaag tgagctcgcg ttacgtcgac tgccagctca
     1321 ccgactcggt gaatcgaacg aatcactacc gctgattcac gagcagccac aaaaccgacc
     1381 cacgccacac ggcgtcttcc gccgagaagt gagtgcactt cgatatgcga caaatcgtcg
     1441 tgtgcacgct tgcgaagtgg cgatgcggtc tgcgaaaatg agtgatcgtg agcctagagg
     1501 gaaagcgagt ccgcatcatg cggcgagtgc tggggagctt tgcgcgtacg cctgcgcgag
     1561 ctcggagact cacgagaggg cgctgaggca cggaggtgtc aggtgtcgat gaatccgaaa
     1621 acgttagagg gacagcttac ggcagggcag atgttgggcc gctacgagat gctcttgccg
     1681 atcgcagcgg gcggtatggg caatgtttgg gcggcgcggc tcaagggcac tcgggggttt
     1741 cgcaagctcg tcgcggtaaa gacggtgctc cgcgcgatca acgacgagaa gctcgagcag
     1801 atgctgtttc aagaagcaac gctcgcttcg cagattcatc accccaacgt ggtcgagaca
     1861 atcgagctcg gcgaacacga tgggacgatg tacttggtga tggagctcgt ctacggcgag
     1921 tcgctgagct tcatcttgcg cgaggcggtc gcgcgcggcg gcgttccgct cacggttgca
     1981 gtcaacctcg tcggtcaggt gtgccgcggg ctgcaagctg cgcacgatct cacggacgcg
     2041 tacggcaatc cggtaggcct cgttcaccgc gacatctcgc cttccaacgt gctcgtgacc
     2101 tatagcggga acgtcaaaat cgtcgacttc ggcgtggcca ctacctccac cacgtcgcaa
     2161 tcgagcagtc ccgagatcaa gggcaagctc tcctacttgg cgccggagca gctgcgcgca
     2221 ggcgaggtcg atgcgcgcgt cgacgtgtac gcgactggcc tgttgctcta cctgatgacg
     2281 gtgcaaaagc acccgttccg cggcgcgagc gagggcgaga cgatcaaccg catcctctcc
     2341 gagcagccgg tgactccgcc ttcggctttc gtcgaagatt accccgaagg gctcgaagca
     2401 gtgctgctga aggctctcga gaagaacagg gaaaaccgct acggctccgc ggcgtctttg
     2461 ctcgaagcgc tcgagtccgc tttacccgag gcgttcggcc cgaacggcga tcgccaaacc
     2521 gtcgagtacc tgcaagagct cctgaagccg cgcatggaag agcgccgcgc gacgctacgc
     2581 atggccgaag atctcgcgga gcagtcttct gcgcgagact ccgcgctgag tttgcccgcc
     2641 atggtctcgc cgccgccttt gccttcgcaa ccgtcgtcgc gcgcgaatcg tttcgccgtc
     2701 ggcgcggcgc tcgtggctgc ctgcgccctc gcgctcggcg ccaagggcat cttcttgaag
     2761 ggcccaaccc agcatcacgc ctccgctgcc atcgctgcag cggtagccgg cgacccggtc
     2821 gcgccgcaaa gcttgccggc accagcggca ccagccgctc cgacttcgct cgtcgcttca
     2881 cccgctacgc ccgaagcgcc tgccagcgcg gcgatcgccg aagagcgcgc tgcacctagc
     2941 ctagcgagcg ctccgcacct cgttgctcac cgcggcgcgc gcggcgaacg ctcggagcac
     3001 ggtgcgggcc cagcggttct cagtgatgtc gctgcttacg acgcaggagc ggccgcggcg
     3061 cccagcgcat cggcgactcc cgaggcggat gctgcggcag cgctccaagt gggctccggc
     3121 ctggcgttgg cgccgagcgc cgaggtcact gcgcagagcg cccaaccgac cacggtaaat
     3181 cctccgccag ccgctcttgc gccatccgcg cccgtcgcaa ccgccgaacg cgacctcgtg
     3241 ccgcgtcgtg tgggctcgcg aatcggcaac gcacgcctcg ccgtgaaccc gggcgccgat
     3301 ggctaccgag cacgcatccc gcgggcgctc gagcgaactc agcagagctt ccgcgcgacc
     3361 gtcgacattt gcgtaaacac cgatggcaag gtgaa
//
LOCUS       DASTKS010000423         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1628778, whole genome shotgun sequence.
ACCESSION   DASTKS010000423 DASTKS010000000
VERSION     DASTKS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628778"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..751)
                     /locus_tag="VFQ35_08860"
     CDS             complement(200..751)
                     /locus_tag="VFQ35_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930784.1"
                     /translation="MRAGEAQRFIEELEQEFPKFRVLYKRESRLCHWIHRALWAITFG
                     GQRHFLQGYYTVIGDTLYVPDSWDALAPADRVVLLRHERVHLRQRRRYGTLGMTFLYL
                     VPWFPLGLAYGRARIEWEAYTETLRATGELLGREAMHDPGLRARIVQRFVGPDYGWMW
                     PFQKSVERWYDAALTAIDRELGG"
     gene            complement(753..1682)
                     /gene="fmt"
                     /locus_tag="VFQ35_08865"
     CDS             complement(753..1682)
                     /gene="fmt"
                     /locus_tag="VFQ35_08865"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET9930785.1"
                     /translation="MRAVFFGTPALAVPSLRALTRIAEVVGVVCQPDRPFGRNQELHQ
                     PEVKVAALALGIPVFQPEKVRDGALATWLLQQRADVALVLAYGRILPKPVLDAPRVGC
                     VNLHASLLPKYRGAAPIQWSIIRGETRTGITLMQVDEGLDSGPMFSHHELAIGPDETA
                     GELAERLAELAAHVVEADLGNVVEGKLRAVPQDDAEVTLAPPLERANGRIDWSRSASE
                     VHNLVRGLAPRPSAHTTLAGKSLKICVTRRASAAPKLRAGEVRVERPRVLVGTGEGAV
                     ELIRAQLEGKRELDAQSLVNGRALNDGDVLGAS"
     gene            1913..>2200
                     /locus_tag="VFQ35_08870"
     CDS             1913..>2200
                     /locus_tag="VFQ35_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9930786.1"
                     /translation="MNEEDIRFGDELPVLPIRNAVLFPGAVAPFDVGREKSVALVEDV
                     HNLQNPVIAIFAQRDPATDDPNADDLYPVGCAARVLKALKHSSGNYSLILQG"
BASE COUNT          378 a          700 c          726 g          396 t
ORIGIN      
        1 accgccacga ccgtgttcgt cgtgccgagg tcgatcccga tcacctgtga catcagctcg
       61 cccttataac gccgtcctcg ccggatttga gccacgaggc gtcgttttcg ggcttcaccg
      121 acgtcggacc gcgtccgtta ccgctagaac cgcgtcgcgg ctcgggaaat ggctccggcc
      181 ggcgtgacgt acgggcagct caaccgccga gctcgcggtc gatggcagtg agcgcggcat
      241 cgtaccagcg ctcgacgctt ttctggaacg gccacatcca gccgtaatcg ggcccgacga
      301 agcgctgtac gatgcgcgcg cggagccccg gatcgtgcat cgcttcgcgc ccgagcagct
      361 cgccggtcgc ccgcaacgtt tccgtgtagg cctcccactc gatgcgcgcg cggccgtacg
      421 cgagaccgag cggaaaccag ggaacgaggt aaaggaacgt catgccgagc gtgccgtagc
      481 ggcggcgttg tcgaaggtgc acgcgctcgt gtcgcagcaa gacgactcgg tcagcgggcg
      541 cgagggcgtc ccaactatcg gggacgtaca gcgtgtcgcc gatgacggtg tagtagcctt
      601 ggaggaaatg tcgttggcct ccgaaggtga tggcccagag cgctcggtga atccaatgac
      661 aaaggcggct ctctcgtttg tagaggacgc gaaacttcgg aaactcttgc tcgagttcct
      721 cgatgaagcg ctgggcctca ccggcgcgca tttcagctcg cgccgagcac atccccgtcg
      781 ttcagggcgc ggccattgac gagcgactga gcgtcgagct cgcgtttgcc ctcgagctgt
      841 gcccggatga gctcgacggc gccttcgccg gtgcccacga ggacgcgtgg gcgctcgacg
      901 cggacttccc ctgcgcgaag tttgggagcg gccgaagctc gccgagtgac gcagatcttc
      961 aggctcttgc ctgcgagcgt cgtgtgcgcg gaagggcgcg gcgcgagccc gcgcacgagg
     1021 ttgtgcactt cgctcgccga acggctccaa tcgatgcggc cgttcgcccg ttcgagcggc
     1081 ggtgcgagag tgacctctgc gtcgtcttgc ggcaccgctc gaagcttgcc ctcgacgacg
     1141 tttccgaggt cggcttctac gacgtgggcc gcgagctcgg cgagccgctc ggctagctcg
     1201 cccgcggttt catcgggccc gatcgcgagc tcgtgatgcg agaacatcgg gccggaatcg
     1261 agcccttcgt cgacctgcat cagcgtgatt ccggttcgtg tctcaccgcg aattatcgac
     1321 cactggatcg gcgcggctcc gcgatacttg ggaagcagcg aagcgtgcag attgacgcag
     1381 ccgacgcgcg gcgcatcgag cacgggcttc ggcagaatgc gcccgtaggc gagcacgagc
     1441 gccacatctg cgcgttgttg gagaagccag gtcgcgagcg ccccatcacg caccttttct
     1501 ggctgaaaaa ccgggatacc gagcgcgagc gccgcgacct tcacctcggg ttgatggagc
     1561 tcctggtttc ggccgaaagg ccgatctggc tggcaaacca cgccaacgac ttctgctatc
     1621 cgcgtgagag cgcggagcga aggcaccgcg agcgctggcg tcccgaagaa aactgcacgc
     1681 acgcccgttt ctatagcact cgcgccgttg gcggacgcat cttctgtggt tacgttcccc
     1741 cgatgatcgc cgttcggaga gtccgcgtga ttgaaattgc gcggtccgcg ttccctgtcg
     1801 cgcgaaagag gcacactgcc tcttctaccg ggtttgcgac ggggtcgagg ggtgacatga
     1861 tgaggttgtc atgagcgaca ataagaagcc gtccccaccg caagcaccgc ccgtgaacga
     1921 agaagacatc cggttcggcg atgagctgcc ggtgcttccg attcgcaacg cggtgttgtt
     1981 ccccggtgca gttgctccat tcgatgtcgg ccgggaaaag tcggtcgctc tggtggagga
     2041 cgtgcacaat cttcaaaacc cggtcatcgc gattttcgcg cagagagacc cggcgacgga
     2101 cgatccgaac gccgacgatc tgtatccggt tggctgcgcc gcgcgggtgc tgaaggcgct
     2161 caagcacagc tccgggaact attcgctgat cctgcagggt
//
LOCUS       DASTKS010000424         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_289361, whole genome shotgun sequence.
ACCESSION   DASTKS010000424 DASTKS010000000
VERSION     DASTKS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289361"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFQ35_08875"
     CDS             complement(<1..368)
                     /locus_tag="VFQ35_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9930787.1"
                     /translation="MHSSTTLSTVDFSRLMPLVAASAERATDAVVEQLDEKLVRATLV
                     EPQTMRADIVTMNSTVLLSCSAWEAPREYRLVYPPERGCKPGELSVLSALGAQLLGAR
                     SGDPDREPHRLMPCITPEPVV"
     gene            complement(1083..1445)
                     /locus_tag="VFQ35_08880"
     CDS             complement(1083..1445)
                     /locus_tag="VFQ35_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9930788.1"
                     /translation="MISATMYVLAVPNLARSAEFYRDVLGFEIREVGDPGWRMFTKDA
                     CRIMAGECPNAIPPSDLGDHSYFGYFVVEGVDQYYEQVRSRGAEVTKPIRDEPWGMRE
                     FGLRTADGHRIMIGSRIV"
     gene            complement(1515..2534)
                     /locus_tag="VFQ35_08885"
     CDS             complement(1515..2534)
                     /locus_tag="VFQ35_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9930789.1"
                     /translation="MSSLEVSAALGALVVAVAGQRGADVERLLAITGFDPKGATDADA
                     RIPLDLEHALWGNAAELARDDAFGVHAAELVKPGMFDVLDYAVRTAPTLRQSLERLVR
                     YNRLVHDAAVWSLVPREGVLRVEHTFSVEGIVQDRHAAEFTLSSIVTIGAQIASMPIR
                     PLAVGFRHAKPASREAQAELTRMFGVEPQFLQPMNYIELDGELVGRENPKADPALSRL
                     IERHAETLLAARPEPTLSTRERVQHVLAITLGEGEAALADVARKLKMSERSLQRRLAD
                     EGVTFDALLDKVRRDLALRYLADPKIAVAEVAFLLGYSEPSPFHRAFKRWTGKTPSEM
                     RKRAA"
     gene            2666..3352
                     /locus_tag="VFQ35_08890"
     CDS             2666..3352
                     /locus_tag="VFQ35_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930790.1"
                     /translation="MSLQPVTRFATFIVALCFSAPAWATPLAGATSEVVAAAEPSRRN
                     PSENAGQHVGVQERDVAPATSARAQRRAFHLDLETDPTAFAFNGYSLHAGLGYRHVRL
                     DLGVYAMDVPGFVETNDGFESSFRGAGAKLQLFLFDEQKGGFVGIDGGMNQLRIQESR
                     TSARDSQWQGSLGVNLGWRFVLPFGFYATPWLGLGYALNAKDMSVAGKTYNPNSFTIF
                     PAVHVGYRFL"
     gene            complement(3337..3897)
                     /locus_tag="VFQ35_08895"
     CDS             complement(3337..3897)
                     /locus_tag="VFQ35_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930791.1"
                     /translation="MVAATAFWAFRAITKTPRTTRFLCSTCLWAALTLLAYAMWQLGP
                     RTSLGRSLVGLVVPLLTLPAALNGRKRWRASLAATLSFCIPACTLRLFVDFLYVGTAH
                     TSGDPVERLCVLLEAVAEFLRENAVTTAIGVLTLFCCFRPNWAPRDRRAFVSATIVFL
                     LATLSVTVTCGGALLVLDGMMLHRNR"
     gene            4015..>4372
                     /locus_tag="VFQ35_08900"
     CDS             4015..>4372
                     /locus_tag="VFQ35_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930792.1"
                     /translation="MPLGPSCPQWVALDECLAKLVRRCRARFAAVIDAGKCVWCSSPD
                     LDIHLQFAAADRFYKVEIKPREGSLRRGAHVVLENLDGPDFYVGESFAAIYFLVIWFA
                     RPRSLDSVREHLRAALP"
BASE COUNT          798 a         1410 c         1367 g          797 t
ORIGIN      
        1 acgacgggtt ccggcgttat gcagggcatc agacgatgcg gctcccgatc gggatctccg
       61 ctacgcgctc cgaggagctg cgcgccgagc gccgacagca ccgacagctc gccgggtttg
      121 cagccgcgtt ccggcgggta aacgaggcga tactccctcg gtgcttccca cgccgaacag
      181 gaaagaagga ccgtcgagtt catcgtaacg atgtccgcgc gcattgtttg cggctcgacg
      241 agggtcgctc tcacgagctt ctcgtcgagc tgctcgacga cggcgtccgt ggctcgctcc
      301 gccgaggctg cgacgagcgg catcaagcga gagaagtcga cagtagatag ggttgtactc
      361 gaatgcatga caaactccga ttgtgagcgc acggccctgc acgccatcgg gtaccaactg
      421 cgcgcgtgcg tcggcgggtc gtcccgcgcg gcgcttccac ttccatgttt cgggcccgcc
      481 aacccgcgac ggctaacgcg tcgagcgcga tggcgctgac accggcgcta ggaaaatgcg
      541 gtcaccggtt ccgcctcgcg cccgtgaaaa agcgcggcgt ttgggcaacc cgccgcgtcg
      601 ctgcgcgaca ggtgttgagt gctcttgcgt tgctaatggc ccgcgaatgg aaaagtcata
      661 ctgcgcgggc acatcctctt gctgcggtgt gcgggctcgt cgttgcggat cagtcgtgag
      721 ccctcataga aagcatcacg agccgcaacg agtcagccaa ccatagtgcg cttatcttag
      781 tgctcacact cgataagtca acccgctcgt cgactctatc tggatatctg gtgagtccag
      841 cgcacggccc gagtcaccct tctctcgacg agcccgcatt ctgccttcga tcgatggatc
      901 gagaacatca cgataaagta acgactcgct cgggaataag ccgggaagaa cgctgcggtc
      961 agaggtgccg gtcgagtgta gtgctctgct gaagcgtggt tggctgcgct aggatgcgcc
     1021 cagccgcctg caaattctct ccgctctact cgtgctcgcc cagtcgcctc gtccggctcg
     1081 gctcagacga tgcggctccc gatcatgatc cggtggccgt ccgcggtacg taggccaaat
     1141 tcgcgcattc cccagggttc atcgcgaatg ggcttcgtga cctccgcacc ccgcgaacgg
     1201 acctgctcgt aatactgatc gacgccttcg accacgaagt acccaaaata ggagtgatcg
     1261 cccaggtcac tcggcggaat cgcattcggg cattcgcccg ccatgatgcg gcacgcgtcc
     1321 ttcgtgaaca tccgccatcc cggatctcca acttctcgaa tctcgaagcc gagcacgtcg
     1381 cgatagaact ccgccgatcg ggcgagattc ggaacggcga gaacgtacat ggtcgctgag
     1441 atcatgggtg agtctagctg tctcgcaggg cgacgtctcg cgagcgcagc ggggcggcac
     1501 cgcgctcggc gcgtttacgc cgctcgcttg cgcatctcgc taggcgtttt tccggtccaa
     1561 cgcttgaaag cgcgatgaaa ggggcttggc tccgagtagc cgagcaagaa cgcgacttcg
     1621 gcgactgcaa tcttcgggtc ggcgaggtac cgcagcgcga gatctcggcg aaccttgtcg
     1681 agcagcgcat cgaaagtcac gccctcgtcc gcgagtcgac gttgaaggct gcgctcgctc
     1741 attttgagct tgcgagccac gtccgcaagc gcggcttcgc cctcgccgag ggtgattgcg
     1801 agcacgtgct gtactcgctc tcgggtgctc aacgtcggct cggggcgcgc ggcgagcagc
     1861 gtctcggcgt gacgctcgat gagtcgcgac agcgctggat ctgcctttgg gttctcccgc
     1921 ccgacgagct cgccgtcgag ctcgatgtag ttcatcggct ggagaaactg cggctcgact
     1981 ccgaacatcc gcgtgagctc tgcctgcgct tcgcgcgacg ctggtttcgc gtgacgaaac
     2041 ccaaccgcga gcggacgtat cggcatggaa gcgatctgcg cgccgatggt cacgatcgag
     2101 ctcaaggtga actcggccgc atgccgatct tgaacgatgc cctcgacgct aaaagtgtgc
     2161 tcgacacgaa gcaccccttc gcgcggcacc agtgaccaga ccgccgcgtc gtgcactaga
     2221 cggttgtaac gcacgaggcg ctcgagcgac tggcgcaaag tcggcgcggt gcgcacggcg
     2281 tagtcgagaa cgtcgaacat gccaggcttc acgagttcgg ccgcgtgcac cccgaaagcg
     2341 tcatcgcggg cgagctcggc ggcgttcccc cagagcgcat gctccaggtc gagtggaatt
     2401 cgtgcgtccg cgtccgtggc ccccttcgga tcgaagcccg tgatcgcgag gagccgttcc
     2461 acgtccgccc cgcgttgtcc ggcgaccgca accaccagcg ctccgagggc ggccgagacc
     2521 tctaagctcg acatgcccca gagatgcctt gaccaaggct cgggggcaac ttgcggtggc
     2581 gcttggggtc agtctttacg gcggctccgg tcacggtcaa accgcggcga tttccccaga
     2641 attggcttcg aaaggaaaga cgcccatgag cctccaaccg gttacccgct tcgccacctt
     2701 catcgttgcc ctctgcttct ccgcccctgc gtgggccacc cccttggccg gcgccacgag
     2761 tgaagtcgtc gcggcggccg agccatcccg tcgcaaccct tccgagaacg caggccaaca
     2821 cgtgggggtg caggagcggg atgtggcgcc cgcgacatcc gcacgagcgc agcgacgagc
     2881 tttccacctc gacttggaga cggatccgac cgcgttcgcg ttcaacggct actccctgca
     2941 cgcgggcttg ggttatcgac acgtgcgcct cgacctcggc gtgtacgcga tggatgtgcc
     3001 cggcttcgtc gaaacgaatg acggtttcga aagttcgttc cgcggcgcgg gcgcaaagct
     3061 ccagctcttc ctgttcgatg agcagaaggg cggcttcgtc gggatcgacg gcggtatgaa
     3121 tcaactgcga atccaggaat cgcgaacttc tgctcgcgac tcccaatggc aaggaagctt
     3181 gggcgtcaac ttgggctggc gcttcgtgtt gcctttcggg ttctacgcga ccccgtggct
     3241 cggcttgggc tacgcgctaa acgcaaaaga catgagcgtc gccggtaaga cgtacaaccc
     3301 gaactcgttc accatcttcc ccgccgtcca cgttggttat cggtttctgt gaagcatcat
     3361 gccgtccaaa accagcaatg cgccgccgca ggtgaccgtg accgacaagg tcgcgagcag
     3421 gaagacgatc gtcgccgaca cgaacgctcg tcggtcgcgc ggagcccagt tcgggcggaa
     3481 gcagcagaac agcgtgagca ctccgatcgc ggtcgtgacc gcgttttctc gcaggaactc
     3541 cgcgaccgcc tccaagagca cacagagcct ttcgacggga tcgccggagg tgtgcgcggt
     3601 tccgacgtac agaaagtcga cgaacaggcg caaagtgcag gcgggaatac aaaacgacaa
     3661 cgtagccgcc aacgaagcgc gccaccgctt tcgaccgttc agcgcggctg ggagcgtcaa
     3721 cagtgggacg accagcccaa ccagcgagcg tcccaggctc gtgcgcggcc ccagctgcca
     3781 catggcgtat gcgagcaacg tcagtgccgc ccagagacag gtcgagcaca ggaatcgcgt
     3841 cgtgcgcggc gtcttcgtga tcgcgcgaaa cgcccaaaac gcggttgccg cgaccatcct
     3901 tcaccagttc ttggggtccg ctaggtgaaa cgcgagggtc gcaggttcct tgggtgaaga
     3961 aatgactagc gcttttgtcc gagcgagcga ggcggcgaag gataccctcc gccaatgcca
     4021 ctcggtccga gttgcccgca gtgggtagcg ctcgacgagt gccttgcgaa gctcgtccgg
     4081 cgctgccgcg cgcgctttgc cgcggttatc gacgccggca agtgcgtctg gtgcagttca
     4141 ccagacctag acatccacct tcagttcgct gcggcggaca ggttttacaa ggtcgaaatc
     4201 aagccgcgcg aaggctcgtt gcgtcgaggc gctcacgttg ttctggaaaa cctggatggc
     4261 ccggatttct acgttggaga atcgtttgcg gcgatctact tcctcgtgat ctggttcgcg
     4321 cggcctagat cgctcgactc cgttcgagag caccttcgcg ccgcgctgcc cg
//
LOCUS       DASTKS010000425         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_513878, whole genome shotgun sequence.
ACCESSION   DASTKS010000425 DASTKS010000000
VERSION     DASTKS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513878"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFQ35_08905"
     CDS             complement(<1..868)
                     /locus_tag="VFQ35_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9930793.1"
                     /translation="MEILSLDALSAIDEELSEEERLVKESVRRFVRERYLPRASELYE
                     NEEFPRDLIPEIADLGLLGASIQGYGCAGMNPVAYGLMLEELEAGDSGLRSFVSVQGS
                     LAMYAIYAFGNEQQKQRYLPEMARGKLIGCFGLTEPDSGSDPGSMKTRARRDGSDWIL
                     NGTKMWITNAPFADVAVVWAKVAEEGDAVRGFIVERGANGFETPTIHGKMSLRSSETG
                     EIVLSDCRVPASSMLENKPGLGAPLRCLNEARFGISWGAVGAAKACFECVQRYSAERV
                     QFGVPIASKQLVQ"
     gene            1024..2835
                     /locus_tag="VFQ35_08910"
     CDS             1024..2835
                     /locus_tag="VFQ35_08910"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9930794.1"
                     /translation="MANSRSAPLHPVPPRFANAKGVDRALTCWFAIAVGCAVFINCSA
                     DRVSRTGSLGSPSPAPGVSQPPLAPPAPAVPLAAEEPKPVPLAATANVAATGDVAATA
                     NVAATVNVAPASPPQEAANATQAALEPTPSTSVSAELAAEPASALEAAPAVPPQPAAA
                     TAPAEPEPNAGPLLYVAAREVLVYAKPNFNSKKLGYLRAGASVTRSAQAQGFERCAKG
                     FYRIAPEGYVCVGDAARLEPSDAVSELADVRPDRTAPLPYVYARSKPPAPPVYVKLPS
                     AADLSSIEPEAVGGRFRVRGVLDALPLSSLPPLLASGHSLPTPFGHEYDRNLVSTGRA
                     LADSAFAITRVFERDHRRYALTTDPSLVPLDRLEPVVASRFHGIALEDAELPVAFVMQ
                     RGAYAYLAEPGKPLRPGRKLEFREALPLSGRTRTDGGARWVETRGGEWLRDERLTRVE
                     MPTALPDAARRGKSWIWVSIGKQALVAFQGTKPVYATLVSTGVDGMGDPETTRSTVRG
                     QFLIHTKHVSVTMSSDEAGAEFELKDIPYVQYFKDNYAIHAAFWHDGFGSPRSHGCIN
                     LAPMDARWLFHWSDPPVPAAWHGAFSLLEGTLVDIEP"
BASE COUNT          495 a         1076 c          880 g          480 t
ORIGIN      
        1 cttgaacgag ctgcttgctc gcgatcggaa caccgaattg gacgcgttcc gccgagtagc
       61 gctgcacaca ctcgaagcag gccttcgccg ctccaaccgc tccccaagag atgccgaagc
      121 gcgcctcgtt caggcaacgc agcggcgcac cgagcccagg cttgttctcg agcatggaag
      181 aagccgggac gcggcaatcg ctcagcacga tttctccggt ctcgctcgag cgcaggctca
      241 tcttgccgtg aatggtgggc gtctcgaagc cgttcgcgcc gcgctctacg atgaagccac
      301 gcaccgcgtc gccttcttcg gcgaccttcg cccagaccac cgcgacgtcg gcgaacggcg
      361 cgttcgtgat ccacatcttc gtgccattca agatccagtc actgccgtcg cgacgcgctc
      421 gggtcttcat cgagcctgga tccgagccgc tatctggctc cgtcaaccca aaacatccga
      481 tgagcttgcc ccgcgccatt tcgggtaagt agcgctgctt ttgctgctcg ttgccgaagg
      541 cgtaaatcgc gtacatggcg agtgagccct gcacgctgac gaagctgcgg agcccactgt
      601 cgcccgcctc gagctcttcc agcatgagcc cgtaggcgac cggattcatc cccgcgcagc
      661 cgtaaccttg gatgctcgcg ccgagtaaac caaggtccgc tatctcggga atgaggtcgc
      721 gcgggaactc ctcgttttcg tagagctcgg aagcccgcgg caagtagcgc tcgcgcacga
      781 agcgccggac gctctctttg acgagccgct cttcctcgga gagctcttcg tcgatggccg
      841 acaatgcgtc gagcgacagg atctccatcg ctcaatgaaa gcaccgttcg cactccgaag
      901 ccgcgaaatc gcgatgtggg cggaagtacg gtcgaggcgc cgcgcggtgc ggccgaacgg
      961 tgcgccagcc gccgcaaact tggccattcg gtagccgatt ggctaggccc gggatcgagc
     1021 cacgtggcaa acagcagaag cgcgcccctt caccccgtcc cccctagatt cgccaacgcc
     1081 aagggcgtcg accgagccct cacctgctgg tttgccatcg ccgtcggttg tgcggtgttc
     1141 atcaactgct ccgccgaccg cgtctcgcgc acggggtcgc tcggcagtcc ctctcccgcc
     1201 ccgggcgtct ctcaaccccc gctcgcacca ccggcgccgg ccgttcccct cgcggcggaa
     1261 gaaccgaagc ccgttccgct cgcggcgacc gccaacgtcg cggcgaccgg cgacgtcgcg
     1321 gcgaccgcca acgtcgcggc gaccgtcaac gtcgcccccg caagccctcc gcaggaagcc
     1381 gcaaacgcta cgcaagccgc cttggagccg acgccctcta cgtcggtgag cgccgaattg
     1441 gcggcggaac cagcctcggc gctcgaggcc gcgcccgcgg tgccgccgca gcccgccgcc
     1501 gccacggcgc ccgccgagcc cgaacccaat gccggcccgc tcctgtacgt cgcggcgcgg
     1561 gaagtgctgg tctacgccaa gccgaacttc aactcgaaga agctcggata tctgcgcgcc
     1621 ggcgcgagcg tcacgcgcag cgcacaagcc cagggcttcg aacgctgcgc caagggcttt
     1681 taccgcattg cccccgaagg ctacgtctgc gtgggcgacg ccgcgcgact cgaaccgagc
     1741 gatgccgtct ccgagctcgc cgatgtcagg cccgatcgca ccgcgccgct tccgtacgtt
     1801 tacgcacgct ccaagccgcc cgcgccgccg gtctacgtga agcttccgag cgcggcagac
     1861 ctctcgagca tcgaacccga agccgttggc ggccgcttcc gcgttcgcgg agtgctcgat
     1921 gcgctgcccc tcagctcact gccgcccctg ctcgcgtcgg gccactcgct ccccactccg
     1981 ttcggacacg aatacgatcg caacctcgtg agcactggcc gcgctctcgc cgatagtgcg
     2041 ttcgccatca ctcgcgtctt cgagcgcgat cacaggcgct acgcgctcac gaccgacccg
     2101 tcgctcgtcc cgctcgatcg cctcgagccc gtcgtcgcct cgcgctttca cggcatcgcg
     2161 ctcgaagacg cagaactccc ggtggcattc gtaatgcaac ggggtgctta cgcctacttg
     2221 gccgaacccg gaaagccgct gcgtccggga cgcaagctcg agttccggga agcgctacct
     2281 ctcagcggaa gaactcgcac cgacggcggc gcgcgctggg tcgagacccg cggcggcgaa
     2341 tggctgcgcg acgagcgact cactcgcgtc gaaatgccga ccgctctccc cgacgctgca
     2401 cggcgcggaa aaagctggat ctgggtctcg atcggcaagc aggcgctcgt cgccttccag
     2461 ggaaccaagc ccgtctacgc gacgctcgtc tcgaccgggg tcgatggaat gggtgaccca
     2521 gagacgaccc gctcgaccgt ccggggacaa ttcctgattc acaccaagca cgtcagcgtc
     2581 accatgagca gcgacgaggc cggcgccgaa ttcgagctca aggacatccc ctacgtgcag
     2641 tacttcaaag acaactacgc gatccacgcc gccttctggc acgacggctt cggctcgccg
     2701 cgaagtcacg gctgcatcaa tttggcgccg atggacgcac gctggctctt ccactggagc
     2761 gatccgccgg tacccgcagc ctggcacggc gccttctcct tgctcgaggg aacgctcgtc
     2821 gacatcgaac cgtagacgac tcgtgcggcg ctgttcgcga gtgtccgtga ctgttcgcga
     2881 gtgtccgtga ctgttcgcga gtgtccgtga ctgttcgcga gtgtccctaa g
//
LOCUS       DASTKS010000426         7163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_959376, whole genome shotgun sequence.
ACCESSION   DASTKS010000426 DASTKS010000000
VERSION     DASTKS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959376"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFQ35_08915"
     CDS             complement(<1..314)
                     /locus_tag="VFQ35_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930795.1"
                     /translation="MGSTHMLRRLAFFGESVRALTQILVIALVLPGCSINAQITDEQS
                     ACERSQRLQDVELYDGSFGVPTSFVDQHRRAVGMLRFRSDLSLRYREEAGNVNGRGWC
                     TGT"
     gene            complement(344..1609)
                     /locus_tag="VFQ35_08920"
     CDS             complement(344..1609)
                     /locus_tag="VFQ35_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930796.1"
                     /translation="MIRATVILLGCANSQDIGSRAAPPENKQPANTPDNTPPYPFNVT
                     PPNGGNAGAGGKGSSASKGGHGGANATGGSMAASATSGGSGSSLGGAAAGGRTMSGSG
                     GTGLAFGSGSAGSSSNPNGCPTPTRALTNSGGCVDRFALFSVGGAPSSIAAVADQTVW
                     FEDERQNELVQLDLSGKIINTLRHSTPASERALIAGSGDTILWFSDPSDRSVSKVSRT
                     LDVFPFGLGIEPAGIALDASDTLWLTEAAQAVYRLQLSSNPDAQRFIATPSGSIAIAD
                     DGTTWYATPTGLAGVADDGTPFDLPLAQVSVSDLCNGPDGAIWFTEVELGRIGRLDGA
                     SLTFYPLPDGGEPHRIIAGPDEAIWFTEQARGSLVRLDLGGEFRDFPIPGGGAEPAGI
                     TVGADGNIWFTQRVAGKVGRLTPDAIFGP"
     gene            complement(1720..3135)
                     /locus_tag="VFQ35_08925"
     CDS             complement(1720..3135)
                     /locus_tag="VFQ35_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HET9930797.1"
                     /translation="MIAHRPLRAFGQHLGLAASIALSIAAIGCGSTAPGTPHGSATDP
                     KAERNDWGDDEEPEASDHDDGKNIADDRDVSDPSDRDDGKDIADDRDASHGDDGNDPS
                     DDRDEPATASGRDGDDKPAGTDPAAKPMSDGSGDEMHESPSPNVPDVASACSAGSEGG
                     RRVIGYVRADVDIWANSFPGSFALDGLTHINLAFAVPNYEAGKYKNLTFAGAGDTYSV
                     QRIKSLVSAAHDKGTKVLASFMGGYASNTDALRAFLGTADDRVASDLVALVKDLDLDG
                     VDIDLEGKTVDGSGGKYANFVELVDQKLDAEFGARCKLLTAAVARWNGDDFPSSALNR
                     FDFISSMSYDAATQRGEEHSTLKLAQDQTAYWVSTRGIPAERVVLGVPFYGYCYGTGC
                     PKETDSEHALSYGPIVRDHPEAKDTDVLQSDTYWITYNGPKTITEKSRYAAQHLGGVM
                     VWTLGHDDAAHSLFSALLEGQ"
     gene            complement(3369..3950)
                     /locus_tag="VFQ35_08930"
     CDS             complement(3369..3950)
                     /locus_tag="VFQ35_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930798.1"
                     /translation="MAGVIRPWFELEAPVLPERVTEQVHRRLTASDCPVGGLTAPGRI
                     ELHVPSQRQQLWSPQLVIDLRRENDVTSVRGEFGPHPYVWILYVAAIALSAIGCLVAA
                     SFGTAQWTMGHTPTAFWAIPLLLVLLLLLYLAAVAGRELSKSEMAELRSFFDGALSDA
                     VALSLREHESVPPSSDAASALIHDTSGPALASH"
     gene            complement(4269..5525)
                     /locus_tag="VFQ35_08935"
     CDS             complement(4269..5525)
                     /locus_tag="VFQ35_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930799.1"
                     /translation="MAASFIPLTLAAASAGCSSSGDDSKGSANGGSSTTAEGGATSSS
                     GGSASTAKGGATSSGGAQNGSGGSSAGANTSGGATTADGPPAGYPDGHAAVPDAGKLE
                     DVSKPTTVVGTGTPASCTAEALVAAVAQGGIITFDCGAEPVTITMSQPAKVFNDKGTK
                     LVIDGGNKVTLSGAGKTRILYMATCNKAQVYPPGSGDCNTNPGVQLVVQNLTFVNGSA
                     KGIDDANNAGGGGAIYAQGGSLKVVNCRFFNNVCDDLGSDVGGGAIRKLDYLVAPGAG
                     PKRPVWVVNSTFGGRMGLGNSCANGGALSSIGVSWNILNSVFSYNVAVGHGANSGQGG
                     NGGAIYNDGNEIVLDIKSSLLENNQANEGGSAVFFVSNDKSGSITLTDSLARNNPRGT
                     FETANLPGFYVIAKQPAQIVNSMILR"
     gene            complement(5678..>7163)
                     /locus_tag="VFQ35_08940"
     CDS             complement(5678..>7163)
                     /locus_tag="VFQ35_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930800.1"
                     /translation="DELMRLGEVARHFRARILVLLAINPLFVNLAFSFMTEFYGFTPA
                     LLGAVIWFRTRRAHAASDGALVGLLPAIASGAVIGGTFWVRQYCVLIFPALVASTLLY
                     VLKTRDWRRLWRSLPSLAASGTAFLALVGGYFWYAKTHNLVKGAFEGRLQDTLHFSLL
                     DYQIMGGAYGFYLTAFLFPLLLTWPLSTRRLSRYTRAGAAAFALALALVTIVELRATD
                     DWASLGVHRVFPYLSNIIHNAGVGPNTLTDGFFYRNDKYYAVSRTFWRNVSYGLLGLS
                     ALWGFALVALASLRNAPRARRELAAFGSAFAALSLVAVVQAYGGGGFDRYSLPCLFGI
                     VLVLGSLASSEEESIHRPLRVRNLIRKWSAIPFALTCLPLAYFTVAGVHDYFRWNDAR
                     WRLVDYALSTGFPSTSINGGYEVNGWLSYDATGLHAVNPDVHRCIGECRCEVPAVLAG
                     LWNCFDDSYRINMSVGPGYDEVMRETPRFWFGRDHSIILSKRRQ"
BASE COUNT         1276 a         2272 c         2335 g         1280 t
ORIGIN      
        1 gtgcccgtac accagcctcg gccgttcacg ttgccagctt cttcgcggta cctgagggag
       61 aggtcactgc ggaagcgaag catccccacc gcgcggcgat gctggtcgac gaagctggtc
      121 ggaactccga agctgccgtc gtagagctcg acgtcttgca acctttgact gcgctcgcac
      181 gccgactgct cgtcggtaat ctgtgcgttg atggagcacc ccgggagcac gagcgcaatg
      241 acgaggatct gcgtcagcgc tcgaacgctc tcaccgaaga acgccagacg tcggagcatg
      301 tgagtcgaac ccatggtcgt ttccttcgga gcaacaggcg ccactacggc ccgaagatcg
      361 cgtcgggcgt gagtcggccg actttgccgg ccacgcgctg tgtgaaccaa atgttgccgt
      421 ccgcgcccac cgtgattccg gccggttccg ccccgccgcc ggggatgggg aagtcacgga
      481 actcaccgcc caggtcgagc cgaacgagcg agccgcgtgc ctgttcggtg aaccagattg
      541 cctcgtcggg gccggcgatg atgcgatgtg gctccccgcc gtccggcaat gggtaaaacg
      601 tgaggctcgc cccatccaac cgtccgatgc ggccgagctc gacttccgtg aaccagatcg
      661 cgccgtctgg accgttgcag agatcgctca cgctgacttg tgcgagcggg agatcgaatg
      721 gagtgccatc gtccgcgacg ccggcgagcc cggtcggtgt cgcgtaccag gtggtgccgt
      781 cgtctgcgat cgcgatgctg ccgctcggcg ttgcgatgaa gcgctgcgcg tcggggttcg
      841 acgaaagctg caagcgatac acggcctggg cggcctcggt cagccacagc gtgtcgctcg
      901 cgtcgagcgc gatgccggcg ggctcgatgc ccaagccgaa cgggaaaaca tcgagggttc
      961 ggctcacctt cgacacgctg cgatcgctcg gatccgagaa ccagagaatc gtgtcgccgg
     1021 agccagcgat gagtgcgcgc tcgctcgccg gcgtactgtg gcggagcgta ttgatgatct
     1081 tgccgctgag gtcgagctgc acgagctcgt tctgtcgctc gtcctcgaac cagacggttt
     1141 ggtcggcgac ggccgcgatg ctcgacggcg cgccaccgac cgaaaacaga gcgaatcgat
     1201 cgacgcagcc tccgctgttg gtcagcgcac gagtcggcgt cgggcagccg ttggggttgc
     1261 tcgacgagcc ggcgctcccg ctgccgaatg ctagcccggt gcctccgcta ccgctcatcg
     1321 ttcgtccccc agcggcagct ccgccgagag agctgccgct gccgccggac gttgcgctgg
     1381 cggccatgct tccgcccgtt gcgttcgcgc cgccatggcc gcctttactt gccgagctgc
     1441 ctttgccccc agcgccagcg ttgccgccat tggggggcgt tacgttgaaa gggtaggggg
     1501 gggtgttgtc gggcgtgttc gcaggctgct tgttttcggg cggcgcagcc cgagacccga
     1561 tgtcttggct gttggcgcaa ccgagcaaaa tcactgttgc ccgtatcatc cacaacgctg
     1621 cgcgagctcg catggttcac cgtcgactgg atcgtgtgcg ttcgccgaaa accgggtgag
     1681 cagagggagg ctgcttacgt cagatttggt gctgacccct cactgtccct ccagcagcgc
     1741 cgagaagagt gagtgcgcgg cgtcgtcgtg tcccagcgtc caaaccatca ccccgccaag
     1801 gtgctgagcc gcgtagcgcg acttctcggt gatggttttc ggcccgttgt aagtgatcca
     1861 gtaagtgtcc gactggagaa cgtcggtgtc tttggcctcg ggatgatcgc gcacgattgg
     1921 cccgtaggag agcgcgtgct ccgagtcggt ttccttcggg cagccggtcc cgtagcagta
     1981 gccgtagaac ggaacgccga gcacgactct ctctgcgggg attccgcgcg tggagaccca
     2041 gtacgcggtt tgatcttgcg ccaacttcag agtcgagtgc tcctcgcccc tttgggtggc
     2101 cgcgtcgtag ctcatggagc tgatgaaatc gaaacggttc aaagcggagc tcgggaagtc
     2161 gtcgccgttc cagcgcgcga cggctgcggt gagcaacttg caccgagcgc cgaactcggc
     2221 gtcgagcttc tggtcgacga gctcaacgaa gtttgcgtat ttgccgccgc ttccatcgac
     2281 ggttttgcct tcgaggtcga tgtccacgcc gtcgaggtcg aggtctttga ccagcgcgac
     2341 taggtcgctt gcgactcggt cgtcagccgt gccaagaaac gccctgaggg cgtcggtatt
     2401 ggaggcgtag ccgcccatga acgacgccag caccttcgtg cccttgtcgt gtgcggcact
     2461 taccagcgat ttgattcgct ggacactata agtgtcgccc gcgcccgcga acgtgaggtt
     2521 cttgtacttt ccggcctcgt agttcggcac cgcgaaggcc aggttgatgt gcgtcagccc
     2581 atcgagcgcg aacgagccgg gaaaggaatt cgcccagatg tcgacgtcgg cgcgaacgta
     2641 gccgatcacg cgtcgcccgc cctccgagcc agcggagcat gcgctagcga cgtcggggac
     2701 gttcggtgag ggtgattcat gcatttcgtc accggagccg tcggacatcg gcttcgcagc
     2761 gggatccgtg ccagcgggct tgtcgtcacc atctcgccca ctggcggtcg cgggctcgtc
     2821 gcggtcgtcg ctcggatcgt tgccgtcatc gccatgcgac gcgtcgcggt cgtcggcgat
     2881 gtctttgccg tcgtcgcggt cggaggggtc ggaaacgtcg cggtcgtcgg cgatgttctt
     2941 gccgtcgtcg tggtccgaag cttctggctc ttcgtcgtct ccccagtcgt tgcgctcggc
     3001 tttcggatct gtggcggagc cgtgcggtgt gcctggcgcc gtcgagccac acccgatggc
     3061 ggcgatggag agggcgatcg aggcggcaag ccccaagtgc tggccaaatg cgcgaagcgg
     3121 tcgatgagcg atcacggtga tacctcctca gtgaaaacga actgaggcag ccgtctcgtg
     3181 cctgggggga gggcacgagc gccgcctcat ccgcactgtg tgagtccgct cgcgatcggg
     3241 tgaagcgtga gcccaccgcg ccttggtacg attttgactt gcgcgcccgg ccgcggaaac
     3301 agtgttggcg gcgagagcaa ggcgactctg ttcagcggcg cagcttgcgg ctcgtcgtgg
     3361 gcgccggctc agtggctagc gagcgccggc cccgacgtgt cgtgaatcaa agcgctcgcc
     3421 gcgtcgctcg acggcggaac cgattcgtgc tcgcgaagcg acagcgcgac cgcatcgctc
     3481 aacgcgccgt cgaagaagct gcgaagctcc gccatctcgg acttactcaa ttcgcgacct
     3541 gccacggccg cgaggtacag caagaggagc aggacgagca acagcgggat cgcccaaaac
     3601 gcagtcggcg tgtgccccat cgtccactgc gcagtaccga agcttgccgc caccagacag
     3661 ccaatcgcgg acagcgcgat cgcggccacg tacaagatcc agacgtaagg gtgcggacca
     3721 aactctccgc gcacgctcgt cacgtcgttt tcacgtcgca agtcgatcac cagctggggg
     3781 gaccagagct gctggcgctg cgaaggcacg tgcaactcga tgcgtccggg cgcggtgagc
     3841 ccacctacgg ggcaatccga agcggtcaag cgccgatgaa cctgttcggt gacgcgctcg
     3901 ggcaataccg gtgcttctag ctcgaaccac ggcctgatga cgccagccat acgccattct
     3961 gcaggcgcga gcgtcgccca gacaaggcgg ctccatcaaa taactacgtg agtcgcgcgc
     4021 ccaatatcga atttgaaaga cgcagcgaaa acgaagcgga cggctttcga cggcggaatg
     4081 ggagcgagtt cacgcccggg cggaaagtga gcaccgatag cggatttctc gatggaatgc
     4141 gacgcagcgt cactctcgcc gtgctcgcaa gcgacgcgac gagtgggtgc aggtgcgcag
     4201 ctcgcctctc tcgatcggtg caccttggcg agcccgggga cgcggcattg gtcgcttgaa
     4261 agcgtggctc agcgcagaat catcgaattt acgatttggg caggttgctt cgcgatgacg
     4321 tagaagccgg gcaggttcgc agtctcgaaa gtgccgcgcg ggttgttgcg agcgagcgag
     4381 tcggtcagcg tgatactgcc cgatttgtcg ttgctcacga agaatacggc gctcccgcct
     4441 tcgttagcct ggttgttttc gagcaagctg ctcttgatgt cgaggacgat ttcgttcccg
     4501 tcgttgtaaa tcgcgccgcc gtttccccct tgacccgagt tggcgccgtg gcccaccgcg
     4561 acgttgtacg aaaagacgct gttcaggata ttccatgaga cgccgatgct gctgagcgct
     4621 ccgccgttcg cgcacgagtt gccgagcccc attcgccccc cgaacgtgct gttcacgacc
     4681 cagactggtc gcttgggacc tgctcccggt gccaccaggt agtcgagctt gcgaatcgcg
     4741 ccgccgccca catccgagcc caggtcatcg cagacgttgt tgaagaagcg gcaattgacg
     4801 accttcaggc tgccaccttg cgcgtaaatg gcgccgccac cgcccgcgtt gttcgcatcg
     4861 tcgattccct tggcgctgcc gttcacgaag gtcaggtttt gcacgacgag ctgcacaccg
     4921 gggttcgtgt tgcagtcgcc gctcccaggc gggtacacct gggctttgtt gcaagtcgcc
     4981 atgtacaaga tgcgtgtctt gccggctccg ctcagagtga ctttgttgcc gccgtcgatg
     5041 acgagcttgg tccccttgtc gttgaacacc ttggcgggtt gactcatcgt gatcgtgact
     5101 ggctccgcgc cgcagtcgaa ggtgatgatg ccgccttgcg cgacggccgc taccaaagcc
     5161 tccgcggtgc agctcgccgg cgtgccggtg ccgactacgg tcgtcggctt cgaaacgtct
     5221 tcgagctttc cggcgtcggg aaccgcggcg tgcccgtctg ggtagcccgc gggtggcccg
     5281 tctgccgtcg ttgcgccgcc actcgtgttc gcgcctgcgc tcgagccgcc agagccattt
     5341 tgagcgccgc cgctcgacgt tgcgccgcct ttcgccgtag acgcggagcc cccggagctc
     5401 gacgttgcgc cgccttccgc cgtcgtcgag gacccgccat tggcagagcc tttcgagtcg
     5461 tcgcccgacg aactgcagcc tgcgctggct gcggcgagcg tgagcgggat gaatgacgcg
     5521 gccaccccaa aatagaatcg cgcgagcatt acctcaagct accgacaacg accaagctca
     5581 gcaacggcgc gactcgacca ccctgtggga aagcgtgaaa cgacgagcgc cacctagcga
     5641 aaacgcgcgc gtttattctg cgcctggagg cagccggcta ctgccgccgc ttcgacaaga
     5701 tgatcgaatg gtcccgccca aaccagaacc gcggcgtttc gcgcatgacc tcgtcgtagc
     5761 ccgggccgac gctcatgttg attcggtagc tgtcgtcgaa gcaattccag agaccagcca
     5821 gcaccgcggg tacttcgcac cggcattcgc cgatgcagcg atgcacatcg gggttcactg
     5881 catggaggcc agtggcgtca tacgataacc agccgttcac ttcgtagccg ccgttgatgc
     5941 tggtgctcgg aaagccggtg ctcaaggcgt agtcgacgag cctccagcgc gcgtcgttcc
     6001 aacggaagta gtcgtgtacg ccagcgaccg tgaagtaggc gagcggcagg caagtgagcg
     6061 caaagggaat ggcggaccat ttgcgaatca agttccttac ccggagcggt cgatggatcg
     6121 actcttcttc actcgacgcg agcgacccaa gcaccaaaac gatgccgaat aggcagggca
     6181 gcgagtagcg gtcgaatccc cctccgccat acgcttgcac caccgcgacc agcgacagcg
     6241 ccgcgaaggc agacccgaaa gcggcgagtt cgcgcctcgc gcggggcgcg ttgcgtaggc
     6301 tcgcgagcgc gacgagcgcg aacccccaga gcgcgctcaa accgaggagc ccgtaactca
     6361 cgttgcgcca gaaggtgcgc gacacggcgt agtacttgtc gttccggtaa aaaaagccgt
     6421 ccgtgagcgt attgggcccg accccggcgt tgtgaatgat gttcgagagg tacggaaaaa
     6481 ctcgatgcac accgaggctc gcccagtcat cggtcgctcg cagctcgacg atcgtcacga
     6541 gagccagcgc gagcgcgaag gcggccgcgc cagcgcgcgt gtagcgtgac agacggcgag
     6601 tgctcagcgg ccacgtgagg agcagcggga acaagaacgc ggtcaggtag aagccgtacg
     6661 cgccccccat gatttgataa tcgaggagcg agaagtgcag cgtatcttgg aggcgaccct
     6721 cgaaagcgcc cttcacgagg ttgtgggtct tggcgtacca aaagtagcct ccaaccagcg
     6781 cgaggaaggc ggttccgctc gcggcgagcg acggcagcga cctccacaac cgacgccagt
     6841 cgcgcgtttt caacacgtag agcagcgtgg aagcgacgag cgcgggaaag atgagcacgc
     6901 agtattggcg cacccaaaac gtaccgccga tcacggctcc tgatgcgatc gccggcaaca
     6961 acccgaccag cgcgccatca cttgcggcgt gggcacgccg ggtccgaaac caaatcacgg
     7021 caccgagcag cgcgggcgtg aagccgtaaa actcggtcat gaagctgaac gcgaggttca
     7081 cgaacagcgg attgatggcg agcagaacca agatgcgggc gcgaaagtgc cgcgccactt
     7141 caccgagccg catgagctcg tcg
//
LOCUS       DASTKS010000427         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1406382, whole genome shotgun sequence.
ACCESSION   DASTKS010000427 DASTKS010000000
VERSION     DASTKS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406382"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1490
                     /gene="ada"
                     /locus_tag="VFQ35_08945"
     CDS             393..1490
                     /gene="ada"
                     /locus_tag="VFQ35_08945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HET9930801.1"
                     /translation="MTQRDPKAPSRSTAAKRDEQRWRQLCERDGSAEGKFIYAVRTTG
                     VYCRPTCASRRPRVENVEFFSTVREAAAAGYRACKRCRPDEAPRPERERALVLKLCRL
                     IETRIASGDAAPSLDELAGLAKLSVFHMHRLFKAVTGLTPKTYAASLRAKRVRGELSK
                     QASVTRAIYEAGFGSSGRFYASSQKILGMKPSAYRAGGNAEKIRFAVGKCSLGAILVA
                     FTERGLCAISLGDDHGALEKELEQRFRKAKLERADATYERQLRRVIALVEKPATGLNL
                     PLDVRGTAFQERVWRALAKIPPGQTLTYTELATIVGAPRAVRAVASACGANPLAVAIP
                     CHRVIGRDGALAGYRWGLDRKRELLDREAES"
     gene            1487..>2188
                     /locus_tag="VFQ35_08950"
     CDS             1487..>2188
                     /locus_tag="VFQ35_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET9930802.1"
                     /translation="MTAKKAELAPAERLLRYDWQKLEEALDLDGCAKLERLLAPGECT
                     ELAGLYDRQELFRSRVVMARHGYGRGEYQYFEYPLPALVGSLREAFYPRLAPVANRWN
                     ESLGVDGRFPAEHREFLESCHAAGQLRPTPLLLRYGESDYNCLHQDLYGDLVFPLQVV
                     VLLSAPERDFTGGEFVLTEQRPRMQSKPLVVPLQQGDALVFAVNQRPVRGARGVYRVK
                     MRHGVSRLRSGHRHTL"
BASE COUNT          384 a          649 c          777 g          378 t
ORIGIN      
        1 gattcgtggc cacgcccggc agtgtacgag cgggaaacgc gagatggggg cacgcagaca
       61 tggcgggcaa cttgtccgaa tgggtgcaag attgggtcgg tgcctaccca gtcccgtgtg
      121 tcaattgcgc cagtatgacg gagactgcga gtacgctggg catcgaacgt ggcggcaact
      181 tccgatcgat cgccgcgacg ttgctcacgt cggaccgcag ccaaacccgg atgctctggg
      241 ttcgtagcag gcagctcgga gcacgttgtg ctcggtcgag atgatacctg tgagacgtcg
      301 cggctaccga tgtggttcgc ggaatttagg ctgtagaatt gaaaagcaag actcggagtg
      361 gcacgaagca ggcttcgcct tagggtctct gtgtgaccca gagagaccct aaggccccga
      421 gtcgaagcac ggcggcgaag cgcgatgagc agcgctggcg acagctgtgc gagcgggacg
      481 gcagcgcaga aggaaagttc atctacgccg tgagaacgac gggggtgtat tgccgcccca
      541 cgtgcgcgtc gcggcgcccc cgcgtcgaga acgtggagtt cttttcgacg gtgcgtgagg
      601 ccgcggcggc gggctataga gcttgcaaac gttgccgccc ggacgaggcg ccgcgcccgg
      661 agcgtgagcg ggcactagtg ctgaagttgt gccgactcat cgagacgcgg atcgcgagcg
      721 gagacgcggc gccgagcctc gatgagctcg cggggctggc gaagctcagc gttttccaca
      781 tgcaccgcct gttcaaggcc gtgacggggc tcacgccgaa gacgtacgcg gcgtcgctac
      841 gagcgaaacg ggtgcgcggt gagctctcga agcaggcctc cgtaacgcgc gcgatttacg
      901 aggccgggtt cggatcgtcg ggtcgctttt acgcgagctc gcagaagatt ctcgggatga
      961 agccgagcgc gtatcgcgcc gggggtaacg cagaaaagat ccgcttcgcg gttggtaagt
     1021 gctcgctggg tgcgatcttg gtggctttca ccgagcgcgg gctgtgcgcg atttcgctcg
     1081 gcgacgacca tggcgctctc gagaaagagc tcgagcagcg ctttcggaag gcgaagctcg
     1141 agcgcgcgga cgcgacgtac gagcggcaac ttcgccgcgt gatcgcgctg gtcgaaaagc
     1201 ccgcgacggg cctgaatctg ccgctcgacg tgcgtggcac ggcgtttcag gagcgcgtgt
     1261 ggcgagcgct ggcgaagatc cctcccgggc agacgttgac gtacacggag cttgcaacga
     1321 tcgtcggtgc gccacgcgcg gtacgagccg tcgcgagcgc atgcggcgcg aacccgctcg
     1381 cggtcgcgat cccctgccac cgcgtcatcg ggcgtgacgg cgccctcgcg ggttatcgct
     1441 gggggctcga ccgcaaacgc gaattgctcg accgcgaggc ggaatcgtga ccgcgaaaaa
     1501 ggccgagctc gctcccgcag agcgattgct gcgctacgac tggcagaagc tcgaggaggc
     1561 gctcgatcta gacggttgcg caaagctcga acggctgctc gcccccggcg aatgcaccga
     1621 gctcgcgggc ctttacgatc gacaagagct ctttcggagt cgcgtggtca tggcccgcca
     1681 cggctacggg cgcggcgagt accagtactt cgagtatcca ctaccggcgt tggtcggctc
     1741 tttgcgcgaa gcgttctatc cacgactcgc gccggtggcg aatcgttgga acgagtcgct
     1801 gggcgtggac ggccgctttc cggccgagca ccgcgaattt ctcgagagtt gtcacgcggc
     1861 cgggcagctt cgaccaacgc cgctgctgtt gcgctacggc gagagtgact acaactgctt
     1921 gcaccaagat ctctatggcg atttggtgtt cccgctgcag gtcgtggtct tgctgtcagc
     1981 gccggagcgc gacttcaccg gaggcgagtt cgttctgact gaacagcggc cccgcatgca
     2041 gtcgaagccc ctcgttgtgc cgctccagca aggggatgcg ctggtgttcg cggtgaacca
     2101 gcgtccggta aggggggcgc gcggggttta tcgagtcaag atgcgacacg gggtgagccg
     2161 gctccgttcc gggcaccggc acacgttg
//
LOCUS       DASTKS010000428         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_514125, whole genome shotgun sequence.
ACCESSION   DASTKS010000428 DASTKS010000000
VERSION     DASTKS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514125"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ35_08955"
     CDS             <1..432
                     /locus_tag="VFQ35_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HET9930803.1"
                     /translation="RASDEAGKEQYLVPYFITGHPGSTLKDTIELAVYLKKNNLRPRQ
                     IQDFIPTPMAVATTMYYTGIDPLNGDPVPVARDLRDKRRMKALILWWDEASWPLAREA
                     LRAAGRSDLIGLGPNALVPPAHHRRPFAPRPVRAHRSRGAR"
     gene            429..1265
                     /locus_tag="VFQ35_08960"
     CDS             429..1265
                     /locus_tag="VFQ35_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9930804.1"
                     /translation="MKSTARVVVISGAASGIGRATALAFARRSDELELVDVNESGLAR
                     VREESLAAGARRVATTHVDVAKEESVSAFAQAFAQRNARCDVLVNNAGVLVAGGFLET
                     EVEDWNFVFDVNLLGAVLLTRALLPGMLGARQGHVINVASASAFWNPSALTAYGCSKY
                     ALVGLSEALRQEVSPQGVRVTAICPSLIATPIVDHMRLRGSLDENESRRRMRDYLERH
                     APKPDLVAQAIVKASMRDVAVVPVGGAAWMLYYLKRLAPGALSRLFGVATKRLLGPDR
                     AE"
     gene            1442..>2396
                     /locus_tag="VFQ35_08965"
     CDS             1442..>2396
                     /locus_tag="VFQ35_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930805.1"
                     /translation="MTTFPPKAEARRRPVSVLALKLWEMAESLEERLDDPTEVLEVLV
                     NSAAKGEQAVETWERLHEAAARFERTSDLALAYEHVTQDRRIKLLSPEQQAFIFMQAA
                     NFFTALGDTEGAAGYAERAISAVPGHAEAFAKLEALLTASGRLGKLAQLYLDASQRES
                     DDAGRLALIQRAFSIAMQIEATDLVIEAGQRLLKLAPEDAGVRDEVMRRLLAGGRHKE
                     VVDLLELALSGDPAPPADEAKLHREQLVDLCFTVLKSPERALNHIEGILRLDPSHAMA
                     QRAAESLLENKQLMLRAAAALSDAFERSGDVERAVRMLTFEL"
BASE COUNT          408 a          789 c          796 g          403 t
ORIGIN      
        1 cgagcgagcg acgaagccgg caaagagcag tacctggtgc cgtacttcat caccggtcac
       61 cccggatcga cgctcaagga caccatcgag cttgcggttt acctgaagaa aaacaacctt
      121 cgcccccgac agattcagga cttcatcccg actccgatgg ccgtggccac gaccatgtac
      181 tacacgggca tcgatccgct gaacggagac ccggtcccgg tggcgcgcga tcttcgcgac
      241 aagcgacgga tgaaagcctt gatcttgtgg tgggacgagg cgagctggcc ccttgcgcgc
      301 gaagctttgc gcgccgcggg ccgcagcgat ctcatcgggc tcggcccgaa cgcgctcgtg
      361 ccgccggctc atcaccgccg cccgttcgcc ccgcgcccgg tccgcgctca tcgctcgcgc
      421 ggagcgcgat gaagtctacg gcgcgggtcg tagtcatcag cggggcggcg agcgggattg
      481 ggcgcgctac tgcgctcgcc ttcgcgcggc gctcggatga gctcgagctc gtggacgtga
      541 acgagagcgg cctcgcgcgc gtgcgggaag aatcgctcgc cgccggcgcc cgccgcgtcg
      601 cgacgacgca cgtggacgtc gcgaaggaag agagcgtcag cgccttcgcg caggcgttcg
      661 cgcagaggaa cgcgcgctgc gacgtgctcg tgaacaatgc cggcgtgttg gtggcgggcg
      721 gctttctgga gaccgaggtc gaagattgga acttcgtctt cgacgtgaac ttgctcggcg
      781 cggtgctgct gacgcgcgct ctcttgcccg ggatgctcgg cgcgcggcaa ggccatgtca
      841 tcaacgtggc gtcggcttcg gcgttttgga atccgtctgc gctcaccgct tacggctgca
      901 gcaagtacgc gctcgtcggg ttgtcggagg ccctacggca agaggtatct ccgcagggcg
      961 tccgcgtcac ggccatttgc ccgagcttga tcgcgactcc catcgtcgac cacatgcggc
     1021 tacgaggtag tcttgacgag aacgagtcgc gtcgacgcat gcgcgactac ctggaacggc
     1081 acgcaccgaa gccagatctc gtcgcgcagg ccatcgtgaa ggcgagcatg cgcgacgtcg
     1141 cggtcgtccc cgtcggcggt gcagcatgga tgctttacta tctgaagcgg ctcgcgcccg
     1201 gggctttgtc ccggcttttc ggggtcgcga cgaagcgctt gctcggacca gaccgcgcag
     1261 agtgacctcg ttgcgcgctt cgcacgacct cgcaccacat gcgtcggtgc tcatccgctc
     1321 agcaacttag ataagtcaca cgctcgcgcg gggggtggcg actttggcga gagcgagctt
     1381 tactcgcggc ccaaagcccg caaaaatggc ggttcaacgc atcgcgcgtt gccatcgcca
     1441 catgacgacg tttcctccga aggccgaagc acgtcggcgt ccagtcagcg tgctcgcact
     1501 caagctctgg gaaatggccg agtcgctcga agagcgcttg gacgacccga ctgaagttct
     1561 cgaggtgctc gtcaactcgg ccgcgaaggg cgagcaggcg gtcgagacct gggagcgctt
     1621 gcacgaagcc gccgcgcgct tcgagcgcac ctccgatctc gcgctcgcgt acgagcacgt
     1681 cacccaggac cggcgcatca agctgctgtc gccggagcaa caagccttca tcttcatgca
     1741 ggcggcgaac ttcttcacgg cgctcggtga cacggagggc gccgccggat acgccgagcg
     1801 ggcgatctcc gccgttccgg ggcacgccga ggctttcgcc aagctcgaag cgctgctcac
     1861 ggcgtcgggc aggctcggca agctcgccca gctctatctg gacgcgagcc aacgtgagtc
     1921 cgacgacgcg ggccgactcg ccctcatcca gcgtgcgttt tcgatcgcga tgcagatcga
     1981 agcaaccgac ttggtgattg aagcggggca gcgcctgctg aagctcgccc cagaagatgc
     2041 gggcgtacgc gacgaggtca tgcggaggtt gctcgccggc ggccgccaca aagaggtggt
     2101 cgacctgctc gagctcgccc tgagcggaga tccggcgcct cctgcggacg aagccaagct
     2161 gcaccgcgaa cagctcgtcg atctctgctt tacggtcttg aagagccccg agcgcgcttt
     2221 gaatcacatc gagggcattt tgcgcctcga tccatcgcac gcgatggcgc agcgcgcggc
     2281 cgagtcgctg ctagaaaaca agcagctcat gctgcgcgcc gcggcggcgc tttcggacgc
     2341 gttcgagcgc tcaggcgacg tcgaacgcgc ggttcggatg ttgactttcg agctgc
//
LOCUS       DASTKS010000429         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1852939, whole genome shotgun sequence.
ACCESSION   DASTKS010000429 DASTKS010000000
VERSION     DASTKS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852939"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFQ35_08970"
     CDS             <1..780
                     /locus_tag="VFQ35_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9930806.1"
                     /translation="RSGRSPKLGTALAIRAAIVTVVAENSHPSVLPILHGSLSVSATR
                     EPPAAPSDMYVAVVDRENELAVALFSARGGGERALVLAREALGVAKVALERRAPMYEI
                     VSALRRLTASERPCHLGAAILRFSQPDARVEILNAGMPPVTCVLPDRSLCLHAALSFG
                     IGARFGEVHPYELAPLTWGSTWLIASDGLTRGERDPGIVRELWETLEVAEQAQAFALA
                     SGEEISRAVQNLLEHDPRSAHGALLAVHADPTRRFRSGIRR"
     gene            802..1344
                     /locus_tag="VFQ35_08975"
     CDS             802..1344
                     /locus_tag="VFQ35_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET9930807.1"
                     /translation="MKIRTTTILRLRDAMLESGRRPSVVVSPAYETLARAGLLSMEET
                     AALSRVEPLAETMFLMVAADGSIADQERDALRGAVRGLSDNLLRTGTINVMLENFEQR
                     LRDHGRDERLREIAEELSDHPSDAESAFTLAAAVALADDEVHDEEQSFINQLAEWLGI
                     NAARAGELLDQLEADRRSVG"
     gene            complement(1280..>2083)
                     /locus_tag="VFQ35_08980"
     CDS             complement(1280..>2083)
                     /locus_tag="VFQ35_08980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9930808.1"
                     /translation="VTLVSTANNHAWDYGRSALIETLYNLNRVGVRSVGTGIDLAEAT
                     RIVSLDVKGMRIAWLAVTHVWNQGPIELHEGRDHVAWADMKALAPIVAEARREHDFVV
                     LSYHGGGEYQDEPAPPTERFVAAAVDAGVDVVIGHHPHVVQGIGFRRGKPAFYSLGNL
                     VFGVRPEHPWTRYGMLAKITLSHASAPKVAICPYGIGDFTPKPFTGPSGDVSRSLFLA
                     HLKRASAFVGGARYGEVDAAGCVEVSPPVIPPSAGPPRADPEARPLARR"
BASE COUNT          361 a          695 c          682 g          345 t
ORIGIN      
        1 agatccgggc gctcgcccaa acttggaacg gcgctcgcaa ttcgcgccgc catcgtgacc
       61 gtcgtcgccg aaaactcgca tccgtcagtc ctcccgatcc tccatggctc gctcagcgtg
      121 agcgcaacga gagaacctcc cgcagcaccc agcgacatgt acgtcgcagt ggtcgatcgg
      181 gaaaatgagc tcgcggtcgc gctgttctcc gctcgcggcg ggggtgagcg cgccctcgtt
      241 ctcgctcgcg aggcgctcgg cgtcgcgaag gtcgcgttgg agcgacgcgc accgatgtac
      301 gagatcgtga gcgcactccg tagattgacc gcgagcgagc gaccttgtca cctcggcgcc
      361 gcgatcctgc gcttctccca gcccgacgcg cgcgtcgaaa tactgaatgc gggaatgccc
      421 ccggtcacgt gcgtgctgcc cgatcgttcg ctctgcttgc acgcggccct atcgttcggc
      481 atcggcgcgc gcttcggaga agtgcatcct tacgagcttg caccgctcac ctggggcagc
      541 acgtggctga tcgcttcgga cgggctcacg cgcggcgagc gcgaccccgg catcgtccgc
      601 gagctctggg agaccctcga agtcgcagag caagcccaag cgttcgcgct ggcgtcaggc
      661 gaggaaatca gccgggcggt gcagaaccta ctcgagcacg atcccaggtc cgctcacggc
      721 gcattgctcg cggtgcacgc cgatccgacg cggcgctttc gctctggaat ccggagatga
      781 gcgcggtgta ccgtagactt catgaagatc cgcaccacga cgatcttgcg cctgcgtgac
      841 gcgatgctgg agagcggacg gcgaccgagc gtcgtcgtct caccagctta cgagacgctg
      901 gcgcgcgcgg ggcttctgag catggaagag acggccgcct tgagccgagt ggaaccgctc
      961 gccgaaacca tgttcttgat ggttgccgcg gacggctcga ttgcagacca agaacgcgac
     1021 gcgttgcggg gcgcggtacg cggactgtcg gacaacttgc tgcgcaccgg caccatcaac
     1081 gtgatgctcg agaatttcga gcagcggcta cgagatcacg gccgggacga gcgtttgcgc
     1141 gaaatcgccg aggagctttc ggaccacccg agcgacgccg agagcgcctt cactctggcg
     1201 gccgcggtgg cgctcgcgga cgacgaggtt cacgacgaag agcagtcgtt catcaaccag
     1261 ctcgcggagt ggctcgggat caacgccgcg cgagcgggcg agcttctgga tcagctcgag
     1321 gcggaccggc gctcggtggg atgacgggcg gagacacctc gacgcaaccc gcggcgtcga
     1381 cttcaccata gcgagcgccg cccacgaaag ccgacgctcg cttgaggtgc gcgagaaaca
     1441 gcgagcgcga gacgtcgccg ctcggcccgg tgaatggctt cggcgtgaag tcgcctattc
     1501 cgtaaggaca gatcgcgacc ttcggcgccg aggcatgcga caaagtgatc ttcgcgagca
     1561 tgccgtagcg agtccacggg tgctctgggc gcacgccgaa taccaggttg cccaagctgt
     1621 agaaggccgg tttgcctctg cgaaaaccga tgccctgcac gacgtgcgga tgatgaccga
     1681 tcaccacgtc cacccccgcg tcgaccgcgg ccgctacgaa ccgctcggtc ggcggcgctg
     1741 gctcgtcctg atactcgccg ccgccatgat agctcagcac gacgaaatcg tgctcgcgtc
     1801 gcgcttcggc cacgatgggt gcgagcgctt tcatgtccgc ccacgcgacg tggtctcggc
     1861 cttcgtggag ctcgatcggg ccctgattcc aaacgtgcgt gaccgcgagc caggcgatgc
     1921 gcattccctt cacgtcgagg ctcacgatgc gagtcgcctc ggcgagatcg atcccggtgc
     1981 cgacgctccg cacgccgact cgattcaggt tgtatagcgt ctcgatcagc gcgcttcgcc
     2041 cgtagtccca ggcgtggttg ttcgcggtcg aaaccagcgt cac
//
LOCUS       DASTKS010000430         4018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1406921, whole genome shotgun sequence.
ACCESSION   DASTKS010000430 DASTKS010000000
VERSION     DASTKS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406921"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VFQ35_08985"
     CDS             complement(<1..1389)
                     /locus_tag="VFQ35_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9930809.1"
                     /translation="MPALLDLDVLVVDCQATGASPTYGSVLEIGWGVVRPNCAEPFDA
                     QAHWVALPQGHVVPRQVQKLTGYEPGANVPSIDAEVAWRRLRAATAHSPATPTAIHFA
                     RFELPFLREWSARFEPEAAFPLDAVCVHAIANRLYPELPRQSLRALAGFLGHSLHLSR
                     RSLGHVEATAFVWRRLSVELSNRGIQSWEDLKAWLAERAPPKARSKRPKYPIDAARYR
                     SLPDQPGVYRFLRCNGDVLYVGKAASLKKRAASHFRGRAGSAQAPEMLTQVADIQVTL
                     VASALEAALLEGDLIKELKPPYNVQLTGAEQGVFYASRDFHSARTEPDAEHALGPLPS
                     EYSLRPLAALAQLLGGSPRSVALRAQAVGVSALWPPDEQVFEAGWETLRERRPKSDFE
                     PHESTRRAALTLARRFLLAPAFAEEDGPEAQAEPPSREWDALRVARHIERACAEAFRA
                     YRRARWLWLLANS"
     gene            complement(1458..3077)
                     /locus_tag="VFQ35_08990"
     CDS             complement(1458..3077)
                     /locus_tag="VFQ35_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET9930810.1"
                     /translation="MREVSMQDGFGEYGFESESGGLSIARRLSVSAAAASLFFGSASA
                     MARDRAPIVRVEQGFLEGTRTGDGLDRFLGVPYAAAPVGERRFRPPQPVPSWRGLRDA
                     TTLPPRCLQLGGGGGGAEDCLYLNVFVPTHTRRAGRLPVIVNLHGGSFKVGAAIDNDP
                     TRLAASTDTIVVMLNYRLGQFGFLAHAALGDESADGSSGNYGFMDQQAALRWVQTNIG
                     AFGGDPTDVTIQGLSAGGWSVCGHLVAEGSRGLFSRAVIHSASCWARPLADAEADGNA
                     FAASLGCTDEANAAACLRAKPSAELLAADSWSQPSSLVYGGGTLPVAPAQSVLAGNFR
                     KVPIIIGGTRNEIRGGLAADYPVDAAKYATLVNEIFGPLASDILAAYPVEQFADPFDA
                     YAEVLNDSSIGGLGTCLTLGLADSFAAHTATYVYEFDDPNAPVPTFYSLPPGMQLGSS
                     HGSDEGYWFDRPFDTLTPLEPLQRLLAHQMNQYLGAFAERGEPVVWRQPRFPRYTASN
                     PAVMRFRPGALGVRNDLGEQHHCALWRSLGF"
     gene            complement(3247..>4018)
                     /locus_tag="VFQ35_08995"
     CDS             complement(3247..>4018)
                     /locus_tag="VFQ35_08995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9930811.1"
                     /translation="GAEPHGRRAFELFGPNAGDAALDVGCGFGDATLELALRVGRAGS
                     VTGIDVAPEFIELAHEHARAEPLAPIDFRVADAERFDDRTRYDYAFSRFGTMFFERPL
                     AAFCNLRNLLRPSGRLVMTVWRPLDENPWLGVAKGVARSFLPSPEPRALSCGPGPFSL
                     AEPTVVRELLGAAGFRQISLAPSDAATLVGRDLHEAIEFQLAIGPAGEIVREAGARAK
                     PLMFELRAALGDALLPYVQREDGGVAIPSAAWIVHARI"
BASE COUNT          722 a         1352 c         1338 g          606 t
ORIGIN      
        1 gctgttcgcg aggagccaaa gccagcgagc gcgtcggtag gcgcgaaacg cctctgcaca
       61 agcccgctcg atgtggcgtg ccacgcgcag cgcgtcccat tcgcgtgagg gcggctctgc
      121 ctgcgcttcg gggccgtcct cctcggcgaa cgccggcgcg agcaagaagc gccgcgcgag
      181 cgtgagcgca gcccgacgcg tagactcgtg tggttcgaaa tcggacttcg ggcggcgctc
      241 gcgcagcgtc tcccagcccg cttcgaagac ctgttcgtcg ggcggccaaa gggcggacac
      301 gccgaccgct tgagcgcgta gcgccaccga gcgcggcgaa ccacccaaca actgcgccag
      361 cgccgcaagc ggccgcaagg agtactcgga aggcagcggg cccagcgcgt gctccgcgtc
      421 cggctcggtc ctggcgctat ggaagtctcg gcttgcgtag aagacgcctt gctcggcgcc
      481 cgtgagctgc acgttgtacg gcggcttcag ctctttgatc aggtcgcctt cgagcaacgc
      541 ggcctcgagc gcactcgcca cgagcgtcac ttggatgtcc gcgacctgcg tcaacatctc
      601 tggcgcttgc gcgctcccag cccggccccg gaagtgactc gcggcccgct tcttcaagct
      661 cgccgctttc ccgacgtaca gcacgtcgcc gttgcaccgc aagaagcgat acacccctgg
      721 ctggtcgggg agcgaccgat agcgcgcggc atcgatcggg tacttgggcc gtttgcttcg
      781 tgccttgggt ggggcgcgct cggcgagcca agctttcaag tcctcccagc tttggatgcc
      841 gcgattcgac agctcgacac tcaaacgccg ccacacgaac gccgttgcct cgacgtggcc
      901 gagcgagcga cgcgacaggt gcaaactatg gcccaggaac ccggccaggg cgcgcagcga
      961 ctggcggggc agctcgggat acaagcggtt cgcgatggcg tggacgcaaa cggcgtccaa
     1021 cgggaatgcg gcttcaggtt cgaaccgcgc gctccattcg cgcaagaatg gcagctcgaa
     1081 gcgcgcgaag tgaatcgcgg ttggggtcgc tggcgagtgc gcggtagccg cgcgcaatcg
     1141 gcgccaagcg acctcggcgt cgatcgaggg aacgttcgcg cccggctcgt agccggtcag
     1201 cttctgcacc tggcgcggca cgacgtgtcc ctggggtagt gccacccagt gtgcctgcgc
     1261 gtcgaacggc tcggcgcaat tcggcctgac tacgccccac ccaatctcga gaaccgaacc
     1321 gtacgtagga ctcgcgcccg tggcctgaca gtcgaccacg agcacatcca agtccagcag
     1381 cgcaggcaca cgacagtgat gagtgcggag gggagctccg tcaagctccc gcctccccgg
     1441 cgagcggtgt cgcgagacta gaagccgagc gaccgccaga gggcgcaatg atgttgctcg
     1501 ccgaggtcgt tgcgaacgcc gagcgcaccc ggacggaagc gcatcaccgc ggggttcgaa
     1561 gcggtgtagc gtgggaagcg cggttgcctc cacactaccg gctcaccgcg ctcggcgaag
     1621 gcacccagat attgattcat ttggtgggcc agcaaccgct gcaacggctc gaggggggtc
     1681 agggtgtcga aggggcgatc gaaccagtag ccctcgtccg acccgtgaga ggagccgagc
     1741 tgcatacccg gcggcaacga gtagaaggta ggcaccggcg cgttggggtc gtcgaactcg
     1801 taaacgtagg tcgcagtgtg cgcggcgaag gaatcggcga gcccgagcgt gagacacgta
     1861 ccgaggcccc cgatgctgct atcgttcagg acctcggcgt aggcgtcgaa cgggtcggcg
     1921 aattgctcga ctgggtacgc ggcgaggata tccgacgcaa gggggccgaa gatctcgttt
     1981 accaacgtgg cgtacttcgc agcgtcgact gggtagtccg cggctaggcc acctcggatc
     2041 tcgttgcgcg tcccgccgat gatgatgggc acttttcgaa agttcccggc aagcactgac
     2101 tgcgccggag ctacgggaag ggtgccgccg ccgtagacga gcgagctcgg ctgcgaccac
     2161 gaatcggccg cgagcagctc ggcgctcggt ttcgctcgga gacacgcggc cgcgtttgct
     2221 tcgtccgtac agccgagaga cgcggcgaaa gcgttaccgt ctgcctcggc atcggcgagc
     2281 ggcctcgccc agcaactcgc gctgtggatc acggcgcgag agaagaggcc cctgctccct
     2341 tcggctacca aatgtccgca cacggaccag ccaccggcag aaaggccctg aatcgttaca
     2401 tcggtcggat cgccgccgaa cgccccgatg ttggtttgca cccagcgcag cgcggcttgc
     2461 tggtccatga agccatagtt gccggagctc ccatccgccg actcgtcgcc aagcgcggcg
     2521 tgcgccaaga acccgaactg acccaagcgg tagttgagca tcacgacgat cgtatcggtg
     2581 ctcgcggcga gccgcgtcgg atcgttgtcg atcgccgctc caactttgaa cgagccgcca
     2641 tgaaggttga cgatcaccgg aaggcgcccg gcacggcggg tgtgggtcgg cacgaagaca
     2701 ttcaaataca agcaatcttc ggctcctccc cctccacctc cgagctggag acagcgcggc
     2761 ggtagcgtgg tcgcgtcgcg cagcccgcgc cacgaaggaa cgggctgcgg tggacgaaaa
     2821 cgccgctcgc cgaccggggc cgctgcgtag ggcaccccca aaaaacgatc gagcccatcg
     2881 ccggttcgcg tcccttcgag aaacccctgc tccactcgta cgatgggagc ccgatctcga
     2941 gccatcgcgg acgcggaacc gaagaatagg ctcgccgcag cagccgatac ggagagccgc
     3001 cgcgcgatgc tgagcccccc gctctccgat tcgaacccgt actccccaaa accgtcttgc
     3061 atcgacactt cgcgcatcgt tccctccgag ctccgtgatg gatttcctcg tctcgcccgt
     3121 acacatgtcg aggcgcgaat agatcgatcc cagcttcgag ttggaggcgc acgcgcacga
     3181 gaccgcttgc ttgatagagt ggctgccgag cgaccacccc ttgcagctgc cacgcgcggg
     3241 ccgagctcag atgcgcgcgt gaacgatcca cgccgcggac ggaatggcga ccccgccgtc
     3301 ttcccgctgg acgtatggca gtagcgcgtc gccgagcgcg gcacgaagct cgaacatcaa
     3361 cggcttcgcg cgcgcgcctg cctcgcgcac gatttcgccg gcggggccga tcgccagctg
     3421 aaattcgatg gcttcgtgca ggtcacgtcc caccaacgtc gcagcgtcgc tcggtgcgag
     3481 cgaaatctgt cgaaaacccg ccgcgccgag tagctcacga acgacggtgg gctccgcgag
     3541 cgagaacgga cccgggccgc agctcagcgc gcgcggttcg gggctgggga gaaacgaccg
     3601 cgctacgccc ttcgcgacgc cgagccacgg gttttcgtcg aggggccgcc agacggtcat
     3661 caccaagcgt ccactcgggc gaagcaggtt ccgcaaattg cagaaggcag cgaggggacg
     3721 ctcgaagaac atcgtgccaa aacgcgagaa cgcataatcg taacgcgttc gatcgtcgaa
     3781 tcgctccgcg tcggccacgc ggaagtcgat cggcgcgagc ggttcggccc gcgcgtgttc
     3841 gtgcgcgagc tcgatgaatt ccggcgcgac gtcgataccg gtgacgctcc cggcccgacc
     3901 aacgcgcagc gcgagctcga gcgttgcgtc gccgaatccg cagccgacat cgagggcggc
     3961 gtcgccggcg ttgggaccga agagctcgaa ggctcggcgc ccgtgtggct cggcgccc
//
LOCUS       DASTKS010000431         5417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_960009, whole genome shotgun sequence.
ACCESSION   DASTKS010000431 DASTKS010000000
VERSION     DASTKS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960009"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFQ35_09000"
     CDS             <1..1047
                     /locus_tag="VFQ35_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930812.1"
                     /translation="AALDADLEFLKEDRCRVDGAALAAELFAQRLRGFARDSPGTLGG
                     VFYVLEGSALGAKQQLGALRVRPELAAGGLEYLTRSAGAAPRFAEFLRRLEAELVDRD
                     AVEAAVTGASATFEAFDSVLTNLLAAPGRIDATLLNGDAGQHAIADDLREIAAALRAG
                     EATYEKYGYYRARYGERGMRFTRSDSAWIASLAARESSVVVRELRWLAGVLAHRGMPR
                     YLLEDHLSVLADELERAVPERAGAYAKLLAARDHLRSERHGLLGEERFRELASGFDRA
                     LPAELDEPLRRSGFVVVAAVLDEASGIAKAVSSVETWFTQQNFPGSLSDAVRETLRAT
                     RRAISSAQPLAEGL"
     gene            1144..>5417
                     /locus_tag="VFQ35_09005"
     CDS             1144..>5417
                     /locus_tag="VFQ35_09005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9930813.1"
                     /translation="MPPSEPSVVVLADDSAMRATTFGDTGEARSCLALFHPVVRAWFE
                     RRFPFGPTPPQAAGWPAIARGEDTLIAAPTGSGKTLSAFLLCIDRLYRARDRGELRED
                     SVEVVYVSPLKALVADIHQNLAEPLDEIRNLAVELGFEPPEIRVGARTGDTKAGARAA
                     LLKRPPHFLVTTPESLYLLLTAARGREMLRTVRTVIVDEIHATARDKRGAHLALSLER
                     LEHVAAERPLRIGLSATQRPLERVARLLVGARSEHGKPRCTLVDVGHRRELDLAIELP
                     DRELEAVASTEQVAEVLDSIAAHAAKRRTTLVFVNTRRLSERLAHLLGERLGGDKVAS
                     HHGSLSKERRARVEQQLRSGELKVLVATASLELGIDIGLVDLVCQVGSPRSIATFLQR
                     VGRSGHSRFAVPVGRLYPLTRDELVECTAMLRAVHAGRLDVLEPPEAPLDILAQQLVA
                     ECAAEEWEEARLFELVRRAGPYMSLERDGFDAALELVSEGIVTGRGTRAAYVQRDRVN
                     GRAKARRGARLAALTSGGAIPESADYRVVAEPEEMFVGTINEDFALESMVGDVFLLGS
                     TSWRVRRVENGTVRVVNAEGAAPTVPFWLGEAPARTRELSEEVSALRAEIEARLGAGD
                     VESAAAELDAQRFVEREARVTSDVARSVVVYLACARKALGLLPTRHDVVFERFFDDTG
                     GMQLVVHSPHGGRVNRALGLLLRKRFCRSFDFELQAAASDDAIVLSLGPQHSFPLEEL
                     RDFLAPAGIDNALAHAVLVTPMFGARWRWNLNRSLVVLRYRGGRRNPPPIQRMEADDV
                     TAAVFPSLAACQDNATGPREIPEHPLVRQTLHDCMHEAMDLTGLRALLAAVAVNEVRL
                     HFRETAEPSPLTHEILNARPYTFLDDAPLEERRTRAVQLRRGLPLEARDLSTLDPAAI
                     ARVRAEVEPVPRDADELHDLLMSLVVMREVDALRTAFESLRAENRALVAEARGGRFWC
                     ALEARGAVIALFGDAVFLPDLPVPPGAELLKSSGGYDRESLLAHALRGRLDVRGPVTA
                     FELAMDLGLLEADVAVGLARLQAEGFALPGRFEAGASGEQYCARRLLSRIHRATQERL
                     RREVEPVTARDFMRFLLRFQGVAPGCQREGQRGVSSVVEQLQGFELAVGSWEGDVLAA
                     RVQGYRPEWLDALSFSGEVVWGRLSSRASVAAGRSATVSRATPITLAFRSDLPWLLTA
                     TRGDDGSRRAPDEAVECSALVACLRERGALFASELAATLGASRAELGEALWDGVARGL
                     ISSDGFGALRSLLGEDRSKGLVTAPSGLRRGASGAISRDGRWALLPSATPATDPDVLA
                     EAVAEQLLARWGVVFADLLGGESFALPYREVVWALRRLEARGRVRGGRFVNGFVGEQY
                     ALPEAVDLLRSVRRVEPSGERVHISACDPLNLTGTILPGARIPAQRG"
BASE COUNT          787 a         1806 c         1896 g          928 t
ORIGIN      
        1 gcggcgctcg acgcagatct cgagtttttg aaagaagatc gctgccgcgt cgatggggcg
       61 gcgctggcgg ccgagctctt cgcgcagcgt ttacgcggtt tcgcgcgcga ctcaccgggc
      121 acactcggag gcgtcttcta cgtcctcgaa ggttcggcgc tcggcgcgaa gcaacagctt
      181 ggggccttgc gtgtgcgccc ggagctcgcg gcgggcggcc tcgaatacct gacgcggagc
      241 gcgggcgcgg cgccgcgttt cgcggagttc ttgcgccggc tcgaggctga gctcgtcgat
      301 agggacgccg tcgaggccgc ggtgacggga gcgagcgcga cgttcgaggc tttcgattcc
      361 gtgttgacga acttgctcgc cgcgccgggg cgcatcgacg ctactctctt gaacggagac
      421 gccgggcagc acgcaatcgc ggacgacctg cgcgagatag cggccgctct gcgggcaggc
      481 gaagcaacct acgaaaagta cggttactac cgcgctcggt acggtgaacg cggtatgcgc
      541 ttcactcgca gcgatagcgc ttggattgct tcgctcgccg cgcgcgagag ctcggtcgtg
      601 gtgcgagagc tgcgctggct cgcgggtgtt ttggcgcacc gcggaatgcc gcgatacctg
      661 ctggaagatc atctgtcggt gctcgccgat gagctcgagc gcgccgtgcc agaacgtgcc
      721 ggcgcatatg ccaagctcct cgccgcgcga gaccatctgc ggagcgagcg gcacggcctg
      781 ctcggcgaag agcgttttcg cgagctcgcg agcgggttcg accgcgcgct ccccgcggag
      841 ctcgacgagc cgttacgcag gagcggcttc gtcgtagtcg cggcggtgct cgacgaagcg
      901 agcgggatcg cgaaggcggt gtcgagcgtc gaaacctggt tcacccagca gaatttcccc
      961 gggtctctca gcgacgcggt gcgtgagacg cttcgagcga cgcgccgcgc gataagctca
     1021 gcacagccgc tcgcagaggg gctctgagcg tacggagtag gccgaagcct cgggaattcg
     1081 agcgatttct ctcccctccg cgctcgccct ggcggcgccg ggcggaggct ctaagatgcc
     1141 gcgatgccgc cgagcgaacc cagcgtcgtc gtcctcgcgg acgattcggc aatgcgcgcg
     1201 accaccttcg gggacaccgg agaagctcgc agctgcctcg cactgttcca ccccgtcgtg
     1261 cgggcgtggt tcgagcgacg cttcccgttc gggccgaccc ctccgcaagc ggcgggctgg
     1321 cctgcgatcg cgcggggaga agacacgctg atagccgcgc ccaccggctc gggaaagact
     1381 ctatcggcgt tcctcttgtg catcgatcgg ttgtatcgcg cccgcgaccg tggggagctg
     1441 cgcgaagact cggtcgaggt cgtgtacgtc tctcctctga aggcgctcgt ggccgacatt
     1501 caccaaaacc tcgccgagcc gctcgacgag attcgaaact tggcggtgga gctcggcttc
     1561 gaaccacccg aaattcgggt aggggcccgc actggcgaca ccaaggcggg ggcgcgcgcg
     1621 gcgctgctca agcgtccgcc ccatttcttg gtcacgacgc cggagtcact ctacctcttg
     1681 ctaacggccg cgcgcgggcg agagatgctg cgcaccgtac gcacggtaat agtcgacgag
     1741 atccacgcga cggctcgtga caaacgcggc gcgcacctcg cgctatcgct cgaacggctc
     1801 gaacacgtcg cggccgagcg ccctctgcgc atcggcctgt cggcgacgca gcgcccgctc
     1861 gagcgggtcg cgcggctctt ggtcggcgct cgcagcgagc acggaaaacc gcgctgcaca
     1921 ctcgtcgacg tgggtcatcg ccgcgagctc gacctcgcca tcgagctacc ggatcgagag
     1981 ctcgaggccg ttgcttcgac cgagcaagtt gcggaagtgc tcgactccat cgccgcgcac
     2041 gccgcgaagc ggcgcacgac gttggtgttc gtcaacacgc gccggctctc cgagcggctc
     2101 gcacacctgc tcggtgagcg cctcggtggc gacaaggtgg cctcgcacca cggtagcctt
     2161 tcgaaggagc gtcgcgcgcg cgtcgagcag caactccgct ccggtgagct caaagtgctc
     2221 gtcgccaccg cttcgctcga gctcggcatc gacatcgggc tcgtcgatct cgtatgccaa
     2281 gtgggctcgc cgcgcagcat cgcgacgttt ttgcagcggg tcgggcgttc tggtcactcg
     2341 cgctttgccg tgccggtcgg caggttgtac ccgctcactc gcgatgagct cgtcgagtgc
     2401 acggccatgt tgcgcgcggt gcacgccggt cgcctcgacg tgttggagcc ccccgaggct
     2461 cccctcgaca tcctggcgca gcagctcgtc gccgagtgcg ccgccgagga atgggaagaa
     2521 gctcggctgt tcgagctcgt tcgtcgcgcc ggcccttaca tgtcgctcga gcgagacggc
     2581 ttcgacgcgg cgctcgagct cgtctccgaa ggcatcgtca ccggccgcgg aacgcgcgcc
     2641 gcgtacgtac aacgcgaccg cgtgaacggg cgcgccaagg ctcggcgcgg ggcacgcctg
     2701 gccgcgctca catcgggcgg cgcgatccca gagtctgcgg actaccgcgt cgtcgcggaa
     2761 cccgaagaaa tgttcgtcgg aaccatcaac gaggatttcg ccctcgagag catggtgggc
     2821 gacgtgtttc tgctcgggag cacgtcgtgg cgcgttcgcc gcgtcgagaa cgggacggtt
     2881 cgggtcgtca acgccgaggg tgccgctcca accgtccctt tttggttggg tgaagcgccg
     2941 gcgcgcaccc gcgagctctc cgaagaggtc tccgcgttgc gcgcggagat cgaggctcgg
     3001 ctcggcgcgg gtgacgtcga aagcgcggcc gcggagctcg acgcccagcg cttcgtggag
     3061 cgtgaagcgc gcgtgacgag cgacgtcgcc cgcagcgtcg ttgtttatct cgcgtgcgct
     3121 cgcaaagcgc tcggcctgct cccgacacgt catgacgtcg tgttcgagcg tttcttcgac
     3181 gataccgggg ggatgcaact cgtcgtgcat tcgccgcacg gcggccgcgt gaatcgcgct
     3241 ctcgggctcc tgcttcgaaa gcgcttttgt cggtcgttcg acttcgagct ccaggccgcg
     3301 gcgagcgacg atgccatcgt gctgtcgctg ggtccgcagc acagctttcc gctcgaagag
     3361 ctacgggact ttctcgcgcc cgctggcatc gacaatgccc tcgcccacgc cgtcctcgtc
     3421 acccccatgt tcggcgcgcg ctggcgctgg aatctgaatc gttccctcgt ggtgctgcgt
     3481 tatcgcggtg gacgaaggaa cccgcctccc atccagcgca tggaagcgga cgacgtgacg
     3541 gcggcggtgt tcccatcgct cgcggcatgc caagacaacg ccaccgggcc tcgcgagatt
     3601 ccggaacacc cgctcgtgcg ccaaacgctg cacgactgca tgcacgaggc gatggatctt
     3661 acggggttgc gcgcgctgct cgcggcggtc gcggtgaatg aagtgcgtct gcacttccgc
     3721 gagacggccg agccttcacc gctgacgcac gaaatcttga atgcgcgtcc gtacactttt
     3781 ctcgacgacg cgccgctcga agagcgccgc actcgagccg tccagctgcg tcgtggattg
     3841 ccgctcgagg cgcgagatct ttccaccctc gacccggccg cgatcgcgcg ggtgcgcgcc
     3901 gaggtcgagc cggtgccccg cgacgcggac gagctgcacg atcttttgat gtcgctcgtg
     3961 gtcatgcgcg aagttgacgc gctccgcacc gcgttcgagt cgctgcgcgc cgagaaccgc
     4021 gctctcgttg cagaggctcg cggcggacgt ttctggtgcg cgctcgaagc gcggggagcg
     4081 gtgatcgcgc tgttcggcga cgcggtcttt ctccccgatc tacccgtccc gcccggcgcg
     4141 gagctactca agagctccgg aggctacgac cgcgagtctt tgctcgccca cgcgctgcgc
     4201 ggccgcctcg acgtgcgtgg gcctgtcacc gccttcgagc tcgccatgga tctcgggctc
     4261 ttggaagccg acgtcgcggt cggacttgct cgactacaag cagaagggtt cgcattgccc
     4321 ggccgcttcg aggcgggcgc gagcggggag cagtactgcg cgcgtcgctt attgagccgc
     4381 atccatcggg cgacgcaaga gcgcctgcga cgggaggtcg agcccgttac ggcgcgagac
     4441 ttcatgcggt tcttgctgcg gtttcagggc gttgcgccgg gctgtcagcg cgaagggcaa
     4501 cggggcgtct cgagcgtcgt cgagcagctc cagggcttcg aactcgcggt aggaagctgg
     4561 gagggggacg tgctcgcggc gcgggtgcag ggctaccgtc cggagtggct cgacgcactt
     4621 tcgttctccg gcgaggtagt gtgggggcgc ttgagctctc gcgcgagcgt cgcggcgggg
     4681 aggagcgcta ccgtctcgcg ggcgaccccc attacgctcg cgtttcgctc cgacttgccg
     4741 tggctgctca ccgccacacg cggggacgac ggctcgcgaa gagcgcccga cgaagcagtg
     4801 gaatgctcgg ctctcgtcgc atgcttgcgg gagcggggcg ctctgttcgc gtccgagctt
     4861 gcggcgaccc tcggcgcatc gagggcggag ctcggcgaag ctttgtggga cggcgttgcg
     4921 cgggggctga tcagctccga cggcttcgga gccttgcgca gcttgctcgg cgaggaccgg
     4981 tcgaagggtc tcgtcacggc cccgagcggc ctccggcgcg gagcgagcgg cgcgatctcg
     5041 cgggacgggc gctgggcgct cttgcccagc gcgacccctg caacggaccc cgacgtcttg
     5101 gcagaagcag tggccgagca actgctagcg cgctgggggg tcgtgttcgc agacttgctg
     5161 ggaggcgaga gtttcgcgtt gccctatcgc gaagtcgtct gggcactacg gcgcctcgag
     5221 gcccgcggcc gcgtgcgcgg tggtcgcttc gtgaatgggt tcgtcggcga gcagtacgcc
     5281 ctgcctgaag cagtcgatct tttgcgcagc gtgcggcgcg tcgaaccgag cggcgagcgc
     5341 gttcacattt cagcgtgcga cccgctgaac ctcactggga ccatccttcc aggagcgcgc
     5401 atccctgcac agcgggg
//
LOCUS       DASTKS010000432         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_736539, whole genome shotgun sequence.
ACCESSION   DASTKS010000432 DASTKS010000000
VERSION     DASTKS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736539"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VFQ35_09010"
     CDS             <1..898
                     /locus_tag="VFQ35_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9930814.1"
                     /translation="DWARALSEATGIDVEFRRSGVLRVAFEDESQAKTLSEHGFQKKH
                     GCKLEALRGAALKRAVPALGPDARAAVRFAEDGRIDPPKLLRALRIAAERHGAEFRSG
                     ALVKRVVTSEGRADGVLLDSGDVIKANKVVVAAGSWSSLVDGVALPEGGVIPARGQIV
                     ELNLSAPVFEPVLFGPKCYLVPRDDGRVLVGSTLEFVGYRREVTAIAVRDLLDAAIQL
                     VPALARATLGSTWSNFRPYTKDGAPFIGEAAIEGILFATGHYRNGILLAPATASLVAA
                     LVLGKKPALKLDAWSPRRGTER"
     gene            969..2009
                     /gene="purM"
                     /locus_tag="VFQ35_09015"
     CDS             969..2009
                     /gene="purM"
                     /locus_tag="VFQ35_09015"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET9930815.1"
                     /translation="MPVTYRDAGVDIDAGEDLVERIKPLAKATRIAEVVSDVGGFAGL
                     CSLPAGIEDPLLVSGTDGVGTKLKLAFAMNKHDTIGIDLVAMNVNDVVTTGARPLFFL
                     DYFGCGKLDVGTAEQVIRGIADGCKQAGCALLGGETAELPDMYPVGEYDLAGFAVGVV
                     SRKKVLGAARVHSDDVLIGVASTGLHSNGYSLARKVLLGSGLELGSHVAELGTTLGDA
                     LLTPTRIYAKAVQALLSALGEDLHALCHITGGGIPGNLPRVLPPNCVAHIEEPALPPL
                     FELIERRGPVERNEMRRTFNCGIGLVVVAKKGSEQAALSALGAAGEKGFVLGRLEAEA
                     GNETARVAYSGG"
     gene            2017..2622
                     /gene="purN"
                     /locus_tag="VFQ35_09020"
     CDS             2017..2622
                     /gene="purN"
                     /locus_tag="VFQ35_09020"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET9930816.1"
                     /translation="MATLELGVLVSGNGSNLQAILDAVQSKQLDARIRLVVSNREQAY
                     ALERARGAGVPAVVCSHRGMASREAYDEKLVSLLRDAGVEYVVLAGFMRILSPVFVRA
                     FHGRVLNIHPALLPSFPGVNALGQALAHGVKVTGVTVHWVDDGVDTGPIIAQRAVAIL
                     PGDDEASLAARVHAVEHELYVEVLRDLAEGKVLPPSPAKEG"
     gene            2623..4191
                     /locus_tag="VFQ35_09025"
     CDS             2623..4191
                     /locus_tag="VFQ35_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET9930817.1"
                     /translation="MPSRYYDAVILGRSLGALATAALLSRRDFRVLVLGQGQRPQTYR
                     FEGRLLCRRPSTLLGASSPAFRRVLQELAYSPQFRRRASNLDPMFSLISEGRRVEVPP
                     DMDLFAREVDREFPEVRQLVDELYTTIASVNAAADAAFERDNVWPPQTLWERFETGRS
                     SYSLPLVRSERTSDLLEKFPRGHAFRELVYVPAQFASNLSGPPDALPPFALARLHGAW
                     TRGILSLSQGEEELIEIFTERIEAQGGVCNLNGRARSIVVRHGTVAGVIEEGEEEPTG
                     AALLVSDQFGEKLADLSGGEGVSKAARRDWPRLTADAGRFVVSVCARRKVLPEPLGSV
                     AFILPEGATRRDPRRPLVRLEQFDPSLFPDARPSARDEVTLVAEAILPARGALTLLEA
                     REAVLQTLREALPFLDEHLICVDSVHDGLPLYDYSSGARREVDRVHVDGTAPTAEPME
                     RLWSVNPMGYLDLAGEPLRGPIPNTFLVGKTVLPALGQEGELIAASSVARIITHRDRA
                     RQRMRRQMWTKIET"
     gene            4196..4777
                     /gene="rsmD"
                     /locus_tag="VFQ35_09030"
     CDS             4196..4777
                     /gene="rsmD"
                     /locus_tag="VFQ35_09030"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET9930818.1"
                     /translation="MSVKVIAGSFKRRVLKTPPGLSTRPTGARVREALFSILGDLGEL
                     EVLDLYAGSGALGIEALSRGAAHATFVENERHALSCIRDNIALLGLDARTSVLSVDVE
                     RLRTAFKSNQRRFDLVFCDPPWAHVERALSNLEELPAWLREGARVVLEHPARRTPELR
                     GFLRVDERTWGDTGMSIFIPESVSSTDRSADAS"
     gene            4884..5465
                     /locus_tag="VFQ35_09035"
     CDS             4884..5465
                     /locus_tag="VFQ35_09035"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9930819.1"
                     /translation="MTSTPPPPPKHGGSGPFIAAAVVMLLLMGGLIYWKFGGGEAPKP
                     APTQAPVAANTPPPPDEPPPPPPPPEEPKDAGVEQKQVKKVVSSTGGGCAGTCSGEEP
                     SSLRSVLAGRAAQARRCYERALMQNSMLQGRMQISVRIGPNGSVCSANLTQNELDPGV
                     GNCVLGIFRSTTFPAPKGGCVDANVPLRFEPKK"
     gene            5465..5686
                     /locus_tag="VFQ35_09040"
     CDS             5465..5686
                     /locus_tag="VFQ35_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930820.1"
                     /translation="MSRFPVTSLLIAASLALPFVHAGCSSEGKTPTCPPLPLYDVRDA
                     GARDAARPALLAAKNCTTLPVGLEGGAGN"
BASE COUNT          943 a         1965 c         1970 g          986 t
ORIGIN      
        1 cgactgggct cgggccctct cagaagcaac cggcatcgac gtggagttcc ggcgcagcgg
       61 cgtgctgcgc gtggcgttcg aagacgagtc tcaagccaag acgctgagcg agcacggctt
      121 tcaaaagaag cacggttgca agctcgaggc tttgcgcggc gcggcgctga agcgggcggt
      181 tccggcgctc gggccagacg cgcgcgcggc ggttcgcttt gccgaagacg ggcgaatcga
      241 tcctcccaag ctccttcgcg cgcttcgcat cgccgcggag cgtcacggcg cggagttccg
      301 aagtggggcg ttggtgaagc gcgtggtgac cagcgaagga cgcgccgacg gagtgctgct
      361 cgatagcggc gacgtcatca aggcgaacaa ggtcgtcgtc gcggccggca gctggagctc
      421 tctcgtcgac ggagttgctc tgccggaggg cggagtgatc ccagcgcgcg gtcaaatcgt
      481 ggagctcaac ttgtcggcgc cggttttcga gccggtgctg ttcgggccca agtgctactt
      541 ggtgccgcgc gacgacggac gcgtgctcgt cggttcgacg ctcgagttcg tgggttaccg
      601 ccgcgaggtc accgcaatcg cggtgcgcga tttgctcgac gccgccatcc aactcgtgcc
      661 ggcgctggct cgcgcaacgc tcgggagcac ctggtcgaac ttccgtccgt acacgaagga
      721 cggcgcgccc ttcatcggtg aagcggcgat cgaaggcatc ctgttcgcga ccggccacta
      781 ccggaacggc atcctcctgg ctccggcaac ggcatcgctc gtggcggcgc tggtactcgg
      841 caagaagcca gcgctgaagc tcgacgcatg gtcgccgcgc cgcggaacgg agcggtgaac
      901 ccaccgtttt ccaaagaaaa tccggccgac tggtatggct cccggttatc ggctatgtag
      961 cgccgcaaat gccggtcact tatcgcgatg cgggcgtcga catcgacgcg ggtgaagacc
     1021 tcgtcgagcg aatcaagccg ctcgccaaag ctacgcggat cgccgaggtc gtctccgacg
     1081 tcggagggtt cgcgggcctg tgctcactgc ccgctgggat cgaagacccg ctgctcgtga
     1141 gcgggacgga cggagtcggc accaagctga agctcgcgtt cgcgatgaac aagcacgaca
     1201 cgatcggcat cgacctcgtc gcgatgaacg tgaacgacgt cgtgacgacc ggcgctcgcc
     1261 cgctcttttt cttggactac ttcgggtgcg gcaagctcga cgtgggcacc gcggagcagg
     1321 tcatccgcgg catcgccgac ggatgcaaac aagccggctg cgcgctgctc ggcggcgaaa
     1381 cggcggagct ccccgacatg taccccgtgg gcgagtacga tctcgccggt ttcgcggtcg
     1441 gcgtcgtttc gcgcaagaag gtgctcggcg ccgcgcgcgt acacagcgac gacgtgctca
     1501 tcggcgtcgc atcgaccggc ctgcattcga acggctactc cctcgctcgc aaggtgctgc
     1561 tcggcagcgg actcgaactc ggctcgcacg tcgcggagct cggcacgacg ctcggcgacg
     1621 ccctgctcac cccaacccgc atctacgcga aggccgtgca agcgctgctg agcgcgttgg
     1681 gtgaagactt gcacgcgctc tgccacatca cgggcggcgg catccccggc aacctcccgc
     1741 gcgtcttgcc gccgaactgc gtggcgcaca tcgaagagcc cgccttgccg ccgctcttcg
     1801 agctcatcga gcggcgcggt ccggtcgagc gcaacgagat gcgtcgcacc ttcaactgcg
     1861 gcatcggtct cgtcgtggtc gcgaagaagg gaagcgagca ggcagctctc tcggcgctcg
     1921 gcgccgccgg cgaaaaaggc ttcgtgctcg ggcggctcga ggccgaggct gggaacgaaa
     1981 cagcgcgcgt cgcctacagc ggaggctgac gcaaacatgg cgacgctcga gctcggtgtg
     2041 ctggtttcgg gcaacggctc gaaccttcag gccattctgg acgccgttca gtcgaagcag
     2101 ctcgacgcgc gcattcgttt ggtggtgagc aaccgggagc aagcgtacgc gctcgagcgc
     2161 gcgcgcggcg ccggcgttcc ggccgtcgtg tgttctcacc ggggaatggc gtcgcgcgaa
     2221 gcgtacgacg agaagctcgt ctcgttgctc cgcgacgcgg gcgtcgagta cgtcgtgctc
     2281 gcgggcttca tgcgcatctt gagccccgtg ttcgtgcgcg cgttccacgg ccgcgtcctg
     2341 aacattcacc cggcgctgtt accgtcattt ccgggcgtga atgcgctcgg ccaagcgctc
     2401 gcgcacggcg tcaaggtgac gggcgtgacc gtgcactggg tcgacgacgg cgtcgacact
     2461 gggcccatca tcgcgcagcg cgcggtcgcg attttgcccg gtgacgacga ggcgtcgctt
     2521 gccgcgcgcg tgcacgccgt ggagcacgag ctctacgtcg aggtcctgcg cgatctcgcg
     2581 gaaggcaaag tcttgccgcc gtcaccagcg aaagaaggct gattgccgtc gcgctattac
     2641 gacgcggtga tattgggtcg ctcgttggga gcgctcgcta cggccgcgct cctctcccgg
     2701 cgcgacttcc gcgtgctcgt actcggacaa gggcagcgac cgcagacgta tcgcttcgaa
     2761 gggcggctcc tctgccggcg gccatcgacg ttgctcggcg cgtcgtctcc agcgttccgg
     2821 cgcgtgctgc aagagctcgc ctacagcccg cagtttcggc ggagggcgag caatttggac
     2881 ccgatgttca gcctgatctc ggaaggacgt cgcgtggagg tcccgccgga catggacctg
     2941 ttcgcgcgcg aagtcgaccg ggagttcccc gaggtgcgcc agctcgtcga cgagctctac
     3001 accaccatcg ccagcgtcaa cgccgccgcg gacgccgcgt tcgaacggga caacgtctgg
     3061 ccgccgcaga ccttgtggga gcgcttcgag accggtcgca gcagctactc gctgccactc
     3121 gtgcgtagcg agcgcacgag cgacttgctc gagaagttcc cgcgcggtca tgcattccgc
     3181 gagctcgttt acgttccggc gcaattcgcg tccaacttgt cggggccgcc cgacgcgctg
     3241 ccgccgttcg cgctcgcgcg tcttcacggc gcttggacgc gcggcatcct ctccttgtcg
     3301 caaggcgaag aggagctcat cgagatcttc accgagcgca tcgaggcaca gggcggcgtg
     3361 tgcaacttga acggccgcgc ccggtccatc gtcgtgcgcc acggcaccgt cgcgggcgtc
     3421 atcgaggaag gtgaagagga gccgaccggc gcggcactgc tggtctccga tcagtttgga
     3481 gaaaagctcg ccgacctgag cggcggagag ggcgtcagca aggccgcgcg ccgcgactgg
     3541 cctcggctca ccgcggatgc ggggcgcttc gtcgtgagcg tttgtgcgcg acgcaaggtc
     3601 ttgccagagc cgttgggcag cgtggctttc atcttgccgg agggcgccac acggcgcgat
     3661 ccgcgtcgcc cgctcgtacg gctcgagcag ttcgatccat cgctctttcc cgacgcgcgc
     3721 ccgagcgctc gcgacgaggt cacgctggtc gcggaggcga ttttgccggc gcgcggcgcg
     3781 ctgacgctgc tcgaggcacg cgaagcggtc ttgcagaccc tgcgcgaggc actgcctttt
     3841 ttggacgagc acctcatttg cgtcgactcg gtgcacgacg gcctgcctct ctacgactac
     3901 tcgagcgggg cgcgccggga ggtcgatcgc gttcacgtcg acggcactgc gcccaccgcc
     3961 gaacccatgg agaggctgtg gtcggtaaac ccgatgggtt atctcgacct cgcgggggaa
     4021 ccactgcgcg gcccaatccc gaacacgttc ctcgtcggta aaacggtgct ccccgcgctc
     4081 ggccaagagg gagagctcat cgccgcctcg agcgtcgcgc ggatcatcac ccatcgcgat
     4141 cgcgcgcgtc agcgcatgcg tcgacagatg tggacgaaga tcgagacttg aggcgatgag
     4201 cgtcaaagtc atcgctggca gcttcaagcg gcgcgtcctg aagactccgc ccggcctctc
     4261 tacgaggcca accggcgcgc gcgtgcggga ggcgctgttc tcgattctgg gagatctcgg
     4321 cgagctcgag gtgctggacc tgtacgcagg cagcggcgcg ctcggaatcg aggcgctctc
     4381 gcgaggagct gcgcacgcca cgttcgtgga gaacgagcgc cacgcgctct cgtgcattcg
     4441 agacaacatc gcgctgctcg gcctcgatgc tcgcacgagc gtgctcagcg tcgatgtcga
     4501 gcgcctgcgc accgcgttca agtccaatca acgccgcttc gatctcgtct tctgcgatcc
     4561 tccctgggcg cacgtcgagc gggctctatc gaacctcgaa gagcttcccg cgtggctgcg
     4621 cgagggcgcg cgcgtcgtgc tcgagcatcc cgcgcgacga acccctgagc tgcgcggctt
     4681 tttgcgcgtc gacgaacgca cctggggcga cactggtatg agtattttca tccccgaatc
     4741 ggtcagctct accgaccgct cggccgacgc cagttgaatg acgattcgcg ctctggcgcc
     4801 caatactcac acgcaagttt gctcgcgctt ggaccgttgg gtcgctatag tcccggggct
     4861 caaggacccc gagggagagc cgaatgacaa gtacccctcc gcctccgccc aagcatggcg
     4921 ggagcgggcc cttcatagcc gccgccgtcg ttatgctcct gttgatggga ggcctcattt
     4981 attggaagtt cggtggcgga gaggcaccca agccggctcc tacccaagcc cctgtcgcgg
     5041 cgaacacgcc accgccgccg gacgagccac cgccgccgcc tcctcccccc gaggagccca
     5101 aggacgccgg cgtcgagcaa aagcaggtca aaaaagttgt ttcgagcacg ggtggcggct
     5161 gtgccggcac ctgctccgga gaagagccgt cgagccttcg ctcggtcctc gcaggccgcg
     5221 cggcgcaagc tcgccgttgc tacgagcgcg cgctgatgca gaacagcatg ctgcaaggca
     5281 ggatgcagat cagcgtccgt atcggcccga acggctcggt ctgcagcgcg aatctgactc
     5341 agaacgaact ggatcctggc gtcggcaatt gcgttctcgg catcttccgc tcgacgacgt
     5401 tccctgcccc caaagggggt tgcgtggacg ccaacgttcc gctcagattc gaaccgaaga
     5461 agtgatgtcg cgcttcccgg tcacgagcct tctcatcgcc gcgagcctcg cgctgccatt
     5521 cgttcatgcc ggctgcagct cagagggtaa gacgcctacc tgtcccccgc tgccgctcta
     5581 cgacgtgcgc gatgcggggg cgcgagacgc cgcgaggccg gcgctgctcg cggcaaaaaa
     5641 ctgcaccact ttgcctgttg ggctcgaagg cggcgccggt aactgataac ggagccgcat
     5701 gccggctgcc gtcttgctca ccgtcgccta cgacgggaga ccgttcgctg gctatgcatt
     5761 gcagcccacc gcgcggacgg tcgccggcga actgaccggc gcgatccggg cgttggaccc
     5821 caaggcgtcg ccgctgcgag gcacgagccg gaccgatgcg ggcg
//
LOCUS       DASTKS010000433         6530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1183113, whole genome shotgun sequence.
ACCESSION   DASTKS010000433 DASTKS010000000
VERSION     DASTKS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183113"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFQ35_09045"
     CDS             <1..567
                     /locus_tag="VFQ35_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET9930821.1"
                     /translation="MVPMIASIGELRAVRALYERARSEVDAAGHKRAEHIPLGMMVEV
                     PSAAILANEFAREAEFFSIGTNDLVQYSLAVDRSSPELAYLASFFDPAILRLIQTVVQ
                     AGRTHNRPVTLCGAMASDQLAAVLLLGLGLRELSMEASAIPAVREAIGEVTLAETDEL
                     VRDVSTLLTAAEIEHCLLDRFAERLDID"
     gene            978..1247
                     /gene="rpsO"
                     /locus_tag="VFQ35_09050"
     CDS             978..1247
                     /gene="rpsO"
                     /locus_tag="VFQ35_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9930822.1"
                     /translation="MPLQPEAKQGVITKFRLHESDTGSPEVQIALLSQRIDYLTEHFK
                     VHRKDHHSRRGLLKLVGQRRRLLDYLKRSDVERYRTVVGSLSLRK"
     gene            1415..3922
                     /gene="pnp"
                     /locus_tag="VFQ35_09055"
     CDS             1415..3922
                     /gene="pnp"
                     /locus_tag="VFQ35_09055"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9930823.1"
                     /translation="MFVRETVVVGGRPLTFETGRLAKLAHGAMLVTFGESVVLVTAVS
                     TDERPGIDFFPLTCEYVEKTYAAGKIPGGFFKREGRLRDVEVLASRLMDRPLRPLFPD
                     GFKKDTQVIATVLSSDKENPTDVLAMCGASAALHVSDIPWDGPIAGVRVARVDGEFIA
                     FPSFDQTAKADIELVVAASKNAIVMVEGGADEASEADLIDALMFAHRAAQPIIELIER
                     MRQAVGKAKRAFTIPKLEQDIARRVAEISDAQLKTACVIREKKARYETYGKIKEELVK
                     KLNEELGAERFLANEKLIKGEFEERKAVVVRTMVLDEGKRIDGRDTRTIRPVSCEVGL
                     LPRVHGSALFQRGETQAIVTTTLGTSSDEQKIDALTGESWKRFMLHYNFPPFSTGETK
                     PMRGPGRREVGHGALAERAVVRMIPGHEEFPYTVRIVSEILESNGSSSMASVCGATLS
                     LMDCGVPIRRPVAGIAMGLIQEGSKVAILSDILGDEDHLGDMDFKVCGTERGVTAIQM
                     DIKIAGLDRAILERALDQAREGRLFILGRMLETLPRARNDINRWAPRITSIKVKPDQI
                     RLIIGPGGKTIKGIVDQTGCSIDVEDDGTVNVASADSDAVQRALHIIEGLVAEPEVGR
                     VYHGTVRRLTDFGAFVEILPNTEALLHVSEMAHERVETPSDVLREGEELDVKVISVDR
                     DGKIRLTRRELLPFPEGEEGERARERIARAREGGPPSRSGGGGGGRGGRDGGGGRGPR
                     EGGQRREGGGHRGEGGGPRSEGGHREGGGGHRGEGGHREGGGGHREGGGHREGGAPRE
                     GGAPREGGAPREGGAPREGGAPQGEGGGRDRSPRPRH"
     gene            complement(4006..5562)
                     /locus_tag="VFQ35_09060"
     CDS             complement(4006..5562)
                     /locus_tag="VFQ35_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930824.1"
                     /translation="MLAIAGACSAPDRDYRDLGSSGSSGSSGGGGKLSVISGGRAGGP
                     NAAGAGGEGGMEPAEGGAPASSGAPGTGGVLGSGGMFAAGAAGAPEPAGAGGELNPGS
                     GGRSEPGAGGATSAGGKTGAGGNISGGGITGTGGLDTTSGGRITIDPCATVLCNTPPA
                     VTCVDAATVRTFGAGTCNAGRCDYPPTDTKCPANQACANGACSVCAVDTQCGPSCSPC
                     ASSAPRCKAAGTGSSCVQCLTSRDCGGNPCDPMTNRCVKGQALLLTNQTSILKFDALT
                     GTSLGVFASGGDLRLAYDLAIGPNGHLYVADYSGNAIREYDGATGAYIASPVSTKAPY
                     NVAFGPDGNMYVGISGGTVYRYSAAGAAIGTFVQGPSGADMGGIRFFGTDLFVSYIGT
                     AGSLVRYDAKGSKVADIYTAFSGNGPRAPSFGPDGTLFVPEWQTPYVKVFAPGSYKYG
                     GNIITDRELSANSIGFAADGAMLVLSDNGNLSSVRRYDPATGTFLGVLVAPGSGGLGR
                     STRMLVLPPN"
     gene            complement(5829..5902)
                     /locus_tag="VFQ35_09065"
     tRNA            complement(5829..5902)
                     /locus_tag="VFQ35_09065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5866..5868),aa:Asp,seq:gtc)
     gene            6107..>6530
                     /locus_tag="VFQ35_09070"
     CDS             6107..>6530
                     /locus_tag="VFQ35_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9930825.1"
                     /translation="MRSALVGAALVVLSAVGFSGKAVLAKVLYRKGVDPLSVIALRMG
                     FSLPVFASLALRNELRSARLSASDLLAVLSLGAVGYYASVYGDFAGLAYISAGLERLV
                     MFTYPTLVLLMAALVHRQRISVLQVGCLVLTYAGIALAF"
BASE COUNT         1125 a         2119 c         2159 g         1127 t
ORIGIN      
        1 atggtgccga tgatcgcgtc catcggcgag cttcgagcgg tgcgggctct ctacgaacgc
       61 gctcgcagcg aagtggatgc cgcgggacac aagcgcgccg agcacattcc actcggcatg
      121 atggtcgagg ttccatcggc cgcgatcctg gccaatgaat tcgcgcgcga ggcagaattc
      181 ttcagcatcg gcacgaacga tctcgtgcag tactcactcg cggtggatcg ctcgagccca
      241 gagctcgcgt atctcgcttc attcttcgat cccgccattt tgcggctgat tcaaacggtg
      301 gtgcaggctg ggcgcaccca caaccgcccg gtcacgctgt gcggtgcgat ggcgagcgat
      361 cagctcgctg ccgtcttgct gctcggcctc ggcctccgcg agctcagcat ggaagcctct
      421 gcgatccccg ccgtccgcga agcgatcggt gaggtgactc tcgccgaaac cgacgagctc
      481 gtgcgcgacg tctcgaccct cctcaccgca gccgaaatcg agcactgcct gctcgatcgc
      541 ttcgccgagc gcctcgacat cgactaggcg tctcaagttc ctgcccgtcg ctcgagccaa
      601 cgcgagagag aagcgcgccc gaggagtggg cacaggagga aggccgaagc ctttcgagga
      661 gcggagcgac gaggccctgc ttcgcagctg caggatcttt gctcgcagca gggcggggga
      721 acttgggact agcggtagct acgcgccgcg cccggcttcg ctggcacctc gccgggctcc
      781 ctcggctcga ggacggagac ctgtaatcgg agcggccatc gggcctaact tcactgaaaa
      841 gcccggttac gattttcatc ccggtttcca attggtatgc ggtccggctg ctgaggacgg
      901 aaatggcgcg gttgaccgat cgatcgcccg gcgtatactc ggcgccccta aacaaaagag
      961 aaggataaga gagacccatg cccctccagc ccgaagccaa acagggcgtc atcaccaagt
     1021 tccgcctcca cgagagcgac accggatctc ccgaggtgca gatcgcgctc ctcagtcagc
     1081 gcatcgacta cctcaccgag cacttcaagg tgcatcgcaa ggatcaccac tcgcgtcgcg
     1141 gcctcttgaa gctcgttggc cagcgccgtc gtctgctcga ctacctcaag cgttcggacg
     1201 tcgagcgcta tcgcaccgtc gtgggttcgc tcagcttgcg taagtaagcg caacgcggaa
     1261 cctggtttcc gggcgtgcgc tgccgaacgc cttcaacttc gcttcgtgcc gccgaaccct
     1321 tcggcggtag gaatcgaggg tgaagccatc ggcgggcgcg ctcggcgttt tgctgcacgt
     1381 agcagcaaca tcgcgccaag gagcgcacaa ttctatgttc gtacgtgaaa ccgtcgtggt
     1441 ggggggacgc ccactcacgt tcgagactgg tcgtttggcg aagctcgctc acggagcgat
     1501 gctcgtcacg ttcggcgaaa gcgtcgtgtt ggtgaccgca gtgtctaccg acgaacgtcc
     1561 aggtatcgac ttttttccgc tgacctgtga gtacgtcgaa aagacgtacg ctgcaggcaa
     1621 aattccgggc ggcttcttca agcgagaagg gcgcctgcgg gatgtcgagg ttctcgcctc
     1681 gcgcctgatg gatcgtcctc tgcgcccgct cttcccggac gggttcaaga aggacacgca
     1741 ggtcatcgcc accgtgctct cctccgacaa ggagaacccg accgacgtgc tcgcgatgtg
     1801 cggggctagc gcggctctgc acgtctccga cattccatgg gacgggccga tcgccggcgt
     1861 gcgcgtcgcg cgcgtcgacg gtgagttcat cgcgttcccg tcgttcgacc agaccgcgaa
     1921 ggcggacatc gagctcgtcg tcgcggcgag caagaacgcc atcgtcatgg tcgaaggtgg
     1981 cgccgacgag gcttcggaag cggacctcat cgacgcgttg atgttcgcgc accgcgccgc
     2041 ccaaccgatc atcgagctca tcgagcgcat gcgccaggcc gtcggcaagg cgaagcgcgc
     2101 gttcacgatc ccgaagctcg agcaagacat cgcgcgtcgc gtggccgaga tcagcgacgc
     2161 tcagctgaag acggcgtgcg tcatccgcga gaagaaggcg cgctacgaga cctacggcaa
     2221 gatcaaggaa gagctcgtca agaagctcaa cgaagagctc ggcgccgaac gcttcctcgc
     2281 caacgagaag ctcatcaagg gtgagttcga ggagcgcaag gccgtcgtcg ttcgcaccat
     2341 ggtgctcgac gagggtaagc gcatcgatgg ccgcgacact cgcacgatcc gtccggtcag
     2401 ctgcgaagtt gggcttttgc cgcgcgtcca cggctcggcg ttgttccaac gcggcgagac
     2461 ccaagcgatc gtcacgacga cgctcggcac ctcgagcgac gagcagaaga tcgacgcgct
     2521 caccggcgag agctggaagc gcttcatgct gcactacaac ttccctccgt tttcgacggg
     2581 ggagacgaag ccgatgcgcg gtccaggtcg ccgcgaagtc gggcacggag cgctcgcgga
     2641 gcgcgccgtg gtgcgcatga tcccggggca cgaggagttc ccctacaccg tgcgcatcgt
     2701 cagcgagatc ctcgagtcga acggctcgag ctcgatggcg agcgtatgcg gcgccacgct
     2761 gagcctcatg gattgcggcg tgccgattcg ccgtcccgtc gcgggcatcg cgatgggtct
     2821 catccaagag ggcagcaagg tcgcgatcct gagcgatatc ctcggcgacg aagatcacct
     2881 cggcgacatg gacttcaagg tctgtggcac cgagcgtggc gtcaccgcca ttcagatgga
     2941 tatcaagatc gcgggtctcg accgtgcgat cttggagcgc gctctcgatc aggcccgcga
     3001 agggcgcctc ttcatcctcg gcaggatgct cgagacgctg ccgcgcgctc gcaacgacat
     3061 caatcgttgg gctccgcgca ttacgagcat caaggtgaag ccggatcaga tccgcttgat
     3121 catcgggccc ggtggcaaaa ccatcaaggg catcgtcgat cagacgggct gctccatcga
     3181 cgtcgaggac gacggcaccg tcaacgtggc gagcgcggac tcggacgcgg tgcaacgcgc
     3241 gctgcacatc atcgaagggc tcgtcgccga acccgaggtg ggccgcgtct atcacggcac
     3301 cgtgcggcgc ttgaccgact tcggcgcgtt cgtcgagatc ctgccgaaca cggaggctct
     3361 gctgcacgtg tccgagatgg cgcacgagcg cgtcgagacg ccgagcgatg ttcttcgcga
     3421 aggcgaagag ctagacgtca aggtcatcag cgtggatcgc gacggaaaga tccgtctgac
     3481 tcgccgcgaa ctcctgccat tcccggaagg tgaagaaggt gagcgcgccc gtgagcgcat
     3541 tgctcgcgct cgtgaaggcg ggccgccctc tcggagcggt ggtggtggcg gcggtcgcgg
     3601 tggccgcgat ggcggcggcg gtcgtgggcc acgtgaaggc ggtcagcgcc gtgaaggcgg
     3661 cggtcaccga ggcgagggcg gcggcccgcg cagcgaaggt ggccatcgcg aaggcggtgg
     3721 gggtcaccga ggcgaaggcg gtcatcgcga aggcggtggg ggtcatcgcg agggcggtgg
     3781 tcatcgcgaa ggcggcgctc cccgtgaagg cggcgctcct cgtgaaggcg gcgctcctcg
     3841 tgaaggcggc gctccccgag aaggtggcgc tccgcagggt gaaggcggcg gccgagatcg
     3901 ttcgccccgt ccgcgtcact gattgagtta ggcgcagcgt gaaagctccg tacggcgcgt
     3961 tgccgtgcgg agctttcgtg cgtttgacgc ctcgagcgcg ctcggtcagt tcgggggcag
     4021 cacgagcatg cgcgtcgagc ggccaagccc accgctgccc ggtgcgacga gcacgcccag
     4081 gaacgtcccc gtcgcgggat cgtagcgacg cactgagctc aggttgccgt tgtccgacag
     4141 aacgagcatc gcgccgtcgg cggcaaatcc gatgctgttc gcagagagct ctcggtccgt
     4201 gatgatgttg ccgccgtact tgtagctgcc gggtgcgaac actttgacgt agggcgtctg
     4261 ccactcgggt acgaacagcg tgccatccgg gccgaaactc ggagcacgcg ggccgttgcc
     4321 gctgaacgcc gtgtagatat cggcgacttt gctgcccttt gcgtcgtagc gaacgagcga
     4381 gcccgcggtg ccgatgtaac tcacgaacag gtccgtcccg aagaaccgga tcccgcccat
     4441 gtccgcgccg gaaggcccct gcacgaaggt gccgatggcc gcgcccgcag ccgaatagcg
     4501 gtaaaccgtt ccccccgaga tgccgacgta catgttgccg tcgggcccga aggccacgtt
     4561 gtaaggcgct ttggtgctta cgggacttgc gatgtaagcg cccgtcgcgc cgtcgtactc
     4621 gcgaatcgcg tttccgctgt agtcggcaac gtaaaggtga ccgttcgggc cgatcgcaag
     4681 atcgtacgcg agccggagat cgccgcccga agcaaatact ccaagtgacg tacccgtgag
     4741 cgcgtcgaac ttcagaatgg aggtctggtt cgtgaggagc agcgcctgcc ccttcacgca
     4801 acggttggtc attgggtcgc agggattgcc gccgcaatcg cgcgaggtca gacattgcac
     4861 gcacgaagag ccagtgcccg ctgccttgca acgtggcgcg gaagaggcac acggcgagca
     4921 gctcgggccg cattgcgtgt cgaccgcgca gacgctgcac gcaccgttcg cacaggcctg
     4981 gttcgcgggg cacttggtat cggtgggcgg gtagtcgcag cggccggcgt tgcaggtccc
     5041 ggcgccaaac gttcgaaccg tcgcggcatc gacgcaggtc acggcgggag gcgtgttgca
     5101 gagcaccgtc gcgcacggat cgatcgtgat gcgtccgccc gaagtcgtgt cgaggccacc
     5161 cgtgccggtg atgccaccgc cgctgatgtt tccgccggcg ccggtcttcc cgccggcgct
     5221 agtggcacca cccgcgccag gctcggatcg tccaccactg cctggattga gttcgccgcc
     5281 agcgcccgcg ggctccgggg ctccagccgc acccgcagcg aacatgccgc ccgagccgag
     5341 cacaccacct gtgcctggcg ccccggagct cgccggcgcg ccaccttcgg ctggttccat
     5401 tccgccttcg ccgccagcgc cggccgcgtt cgggccgccc gctcggccac cagatatgac
     5461 cgacagcttg ccgccgccac cgctggagcc actcgagccg ctcgagccca agtcgcggta
     5521 gtcgcgatca ggcgctgaac acgctcccgc gatcgccaac acgaaaccta ccgagcacgc
     5581 ttgccgctga ggtcgcctca taaccctgcg agcaagctgc acggccccga tcggcccgtc
     5641 aagcggcatt ggcgcgcttc gctgcagacg agcgtgcgag cgcgagttcg cgcggctcgg
     5701 gcgcagacgc gacacctggt tgcgttctcg ctcgagcctc tcgaactttg cgaaactatc
     5761 acctaggagc gctgtggcac tttgaccacc acagcggcgg gcgcattcgc tttttcagag
     5821 tatcgttgcg gggcggacgg gactcgaacc cgcggcctcc ggcgtgacag gccggcgtta
     5881 taaccaactt aactaccgcc ccgaagcggc gggcgggtaa tagcacaagg tcccccgtcg
     5941 tccacaagtg gaaacgcaca tctgcttttt tgtttatggc acccaaggct ggccatttgg
     6001 ccggccgttt tcgcagcgaa cgcggggact tgagctcgca ccacgagcct cgggttagag
     6061 tcgccgtcgc gtgagcgagt cagtcgggcc gcgctctcag cgatcagtgc gaagcgctct
     6121 cgtaggggcc gctctcgtcg tgctgtcggc ggttgggttt tcgggcaaag cggtgctcgc
     6181 gaaggttctc taccgaaagg gagtggaccc actcagcgtg atcgcgttgc gcatggggtt
     6241 ctcactgccg gtcttcgcat cgctcgcgct gcgcaatgaa ttgcgttcgg cgcggctttc
     6301 ggcctccgac ctgctcgcgg tgctctctct tggagccgtc gggtattacg cctccgttta
     6361 cggggacttt gcggggctcg cgtacatctc ggcggggctc gagcggctcg tcatgttcac
     6421 gtacccgacc ctcgtgcttt tgatggccgc actcgttcat cggcagcgaa tttcggtcct
     6481 gcaggtgggc tgcctcgtgc tcacctacgc gggcatcgcg ctcgcgttta
//
LOCUS       DASTKS010000434         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_68503,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000434 DASTKS010000000
VERSION     DASTKS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68503"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFQ35_09075"
     CDS             complement(<1..374)
                     /locus_tag="VFQ35_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930826.1"
                     /translation="MFLDTGARGILALAFFAALSCLVGSACTTALWLERSEQPGVVLS
                     REVPIVVHVSDRVIELDDEGNVDALVDALEEGLIEKGFEFRIIGLKAEVDGGSARVPV
                     YPGRLPLPRVELAVSAHDESVD"
     gene            complement(486..1469)
                     /locus_tag="VFQ35_09080"
     CDS             complement(486..1469)
                     /locus_tag="VFQ35_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930827.1"
                     /translation="MGRTRLLARCLAAGVLWACAGATSTSGGAGGGSGATGAFESGGK
                     STLAGNAGTGGSAAGGHDVATAGVGSGKGGYSGSSGGSLGGGGRFDVTTGGRYEGVAG
                     GSVVGWCPGEPVDPTNACSDDVACNAGLVCVSVAAARQVPVSPCMPYITQPELACTSR
                     SSPGPSTLCPAPKSCLRGACGRLSCQPTCPDPIQCGAGYACEQGVCVLKRCDTPSGTT
                     CEEGYRCDPANTTAASGCAPISCTEGYVCPAGSVCRPVTGEEHGCHVLTCAEGVPCPA
                     GYDCTNMASPNDARCSLRACKHSTDCACGSCVQGYCDLVPGRCTVMGYGGS"
     gene            1677..>2919
                     /locus_tag="VFQ35_09085"
     CDS             1677..>2919
                     /locus_tag="VFQ35_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9930828.1"
                     /translation="MADRPPRERLLTFLLLPAIVLAVLVLASVMFRSSFQLERLREQS
                     VVEATLLLANEKADRLDKRIIEQDNAVRSVLDVGEREHFGDKWLESAALQTPTVRAVL
                     LIDLVAPTHPVVAAASRAPGPENERFRRLVVLSMLDDMKLDRPPLGELRHLHKSYRGQ
                     SYLISHWDAVNAERRYLVVVWHDVPRIVHDLFPELYSDVQQSRVNVVDSDGRIVFGPP
                     LSRGGLTLGRQFETTLYKWTLNVTMNSAAELAAAVARRRVLEMALVGLSGVVVIAGII
                     VIVLAAERERKLSNLKSDFVANVSHELKTPLALVRMFGELLQSGRADSEQKRQQYLQI
                     IVSESDRLASLIENVLDFAKVERGRAAYEFELAAVGDVVARAVEACRVRAEREGTVLT
                     VDSVPDLPSAWLDQRAIEIAVI"
BASE COUNT          494 a          987 c          944 g          494 t
ORIGIN      
        1 ctgtcgacgc tctcgtcgtg agcgctcacg gcgagctcca cccgcggcag cggtagtcgc
       61 cccgggtata cggggacgcg agccgacccg ccgtcaactt ccgccttgag cccaatgatt
      121 cgaaactcga aacccttctc gatgagcccc tcttcgagcg cgtctaccag cgcatcgacg
      181 ttaccttcgt cgtcgagttc gatgacgcga tcgctcacgt ggacgacgat gggaacctcg
      241 cgcgacagga cgacgccggg ttgctcgctg cgttcgagcc agagggcggt cgtgcaggca
      301 ctgccgacta gacacgagag cgcggcgaag aaggcgaggg cgagaatgcc gcgggcaccg
      361 gtatccaaga acacggcaga atctcaatcg agtccagaca actaagtcaa gcgatgcatc
      421 cggagcgcgc agccgcgagc tcctgtcggc gctcgtcgtc gtgacgcgag gggagcgcgc
      481 ctggctcagg acccaccgta tcccatgacc gtgcaacgac ctggcaccag atcgcagtag
      541 ccttgaacac agctcccgca cgcgcaatcg gtcgagtgct tgcacgcgcg gagtgagcac
      601 ctggcatcgt tcggcgacgc catgttggtg cagtcatatc cggcagggca cgggactcct
      661 tcggcgcagg tgagcacgtg acagccgtgt tcctcgccag tgaccggcct gcacaccgag
      721 ccagcgggac agacgtaccc ttcggtgcac gagatcggag cgcagccgct cgcggcggtc
      781 gtgttggctg gatcgcagcg atagccttct tcgcaggtgg tgccgctggg ggtgtcgcag
      841 cgcttcagta cgcagacgcc ttgctcgcag gcgtaaccgg cgccgcactg gattgggtca
      901 gggcaagttg gctggcacga cagccgtccg caggctccac gcaagcagct ctttggggct
      961 ggacacaacg tgctcggccc cggcgaggat cgcgacgtgc aagcgagttc cggttgagtg
     1021 atgtacggca tgcagggaga aaccggaact tggcgcgctg ccgctaccga aacgcaaacc
     1081 aaacccgcgt tgcaggccac gtcgtccgag caggcgttgg tagggtcgac aggctcgcca
     1141 gggcaccacc ccactacgct tccgccggcg acgccttcgt atctacctcc ggtcgtgacg
     1201 tcgaagcgtc ctcctccgcc cagcgagccg ccgctgctgc cgctgtaccc tccttttccc
     1261 gagcccaccc ccgcggtcgc gacgtcgtgt ccgcccgccg ctgagcctcc agtgcccgcg
     1321 ttgcctgcga gtgtgctctt accgccggac tcgaaggcgc cagtcgcgcc gctgccgccg
     1381 cccgcgccgc cggacgtcga agtcgccccc gcgcaagccc agagcacgcc cgccgccaga
     1441 caacgcgcga gcagtcgcgt tcttcccatg gcttggctgg cagcaaagct cgtgccccgc
     1501 tgcgaacctc tagaagacgc tcatttcatg gtgttgtgcc acgggcgtcg gcacactctg
     1561 cgccgcacgg gtgcccacgg agcaaagctc acctcgcacg ggcgtacgag ggcggcgctg
     1621 gctatcgacg gacggagttc ttgcaaaccc cgcacgccgg ctaaggtatg cggccaatgg
     1681 ccgaccggcc cccccgcgag cgcctcctca cgttcttgct cctgcccgcg atcgtgcttg
     1741 cggtgttggt gctggcgagc gtgatgtttc ggtccagctt ccagctcgag cggctgcgag
     1801 agcagtcggt cgtggaagcg acgctgttgc tcgcgaacga gaaggcagat cgactcgaca
     1861 agcgcatcat cgagcaagac aacgccgttc gttcggtgct cgacgtgggg gagcgcgagc
     1921 acttcggtga caagtggctc gaatccgccg cgctgcagac accgaccgtg cgcgccgtgc
     1981 tgctcatcga tttggtcgcg ccgactcacc cggtggtcgc ggctgcgtcg cgcgcccccg
     2041 gaccggagaa cgagcgcttt cggcgcctgg tcgtgctctc gatgctcgac gacatgaagc
     2101 tcgaccggcc gcccctcggc gagctcagac accttcacaa gagctatcga ggccagagct
     2161 acctcatcag ccattgggac gcggtgaacg ccgagcgccg ctacctcgtg gtggtttggc
     2221 acgacgtgcc gcgcatcgtg cacgaccttt ttccggagct gtactccgat gttcagcaga
     2281 gccgcgtgaa cgtcgtcgat agcgacggcc gtatcgtctt cgggcctccg ctcagccgcg
     2341 gtgggctcac gttgggtcgc cagttcgaaa cgacgctcta caagtggaca ctcaatgtga
     2401 cgatgaactc cgccgccgag ctcgccgcgg cggtcgcgcg acggcgcgtg ctcgaaatgg
     2461 cgttggtcgg cctttccggc gtcgtcgtca tcgccggcat catcgtcatc gttttggcgg
     2521 ccgagcgaga gcgaaagctt tcgaatctga agagcgactt cgtcgcgaac gtctctcacg
     2581 agctgaagac tccgctcgcg ctcgttcgaa tgttcggcga gctcttgcag tcggggcgcg
     2641 ccgacagtga gcagaagcgc cagcaatacc ttcaaatcat cgtgtcggag agcgacaggc
     2701 tcgcgagctt gatcgagaac gtactcgact ttgccaaggt cgaacgcgga cgcgccgcct
     2761 acgaattcga gctcgcggcc gtcggcgacg tcgttgcgcg cgccgtcgag gcctgccgcg
     2821 tgcgcgccga gcgcgaaggc accgtcttga cggtggattc ggttcccgac ctaccgagcg
     2881 cgtggctcga ccagcgcgcg atcgagatcg cggtgatca
//
LOCUS       DASTKS010000435         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_290515, whole genome shotgun sequence.
ACCESSION   DASTKS010000435 DASTKS010000000
VERSION     DASTKS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290515"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VFQ35_09090"
     CDS             complement(<1..1706)
                     /locus_tag="VFQ35_09090"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HET9930829.1"
                     /translation="MRLLGLACWVAGLCVVACSDATAPSPFDDLQAGSGGEAGAGAAP
                     STGGRLSTGGGPNEWGGVCQESAQCDDGLECTEDSCDAASGRCRFSPVDTRCDNDVFC
                     DGIEQCLPGIGCRPGGSVACDDGTTCTIDRCIEETRSCEHLVRDADGDGDGVWNCAGG
                     GDCDDTDPKVSSLTREVCANQRDDDCDGESDEGDCTTPAHDTCVDALVIEASGTYGLN
                     LEGAGEDYGLSCARSVPARRDVVAAIRIPEGEAVDVDVAVLAADAELALAIAASCSRK
                     GSELSCARAAALESQSFAARLWLRGFEPGVYPVFVSGASESPLELRVHYGEAKPAPEN
                     ETCGAAASIESGARAVASLAEAARDVSGACAESYSELLYRFELESQSDVIASAWPLDD
                     LGSPTLSLRRAACADETDELDCRVGSPARLFERALEPGEYFLAVGSTGPGDVELHFDV
                     QPATAPEPGELCSEAPAITPGSTEIVEFAKGTSSVAVECLAGAVDTTRSLPLTETSDV
                     LLVERLSSGDTGAVALLDRECRSRPAWACESGAESPVRARAFEVPAGEYRVVAESQRG
                     SPV"
     gene            1852..>2704
                     /locus_tag="VFQ35_09095"
     CDS             1852..>2704
                     /locus_tag="VFQ35_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930830.1"
                     /translation="MSNERLTRHEITWLLAQEARGAARTLRSEVGRVSMPSAVPAPGT
                     EPVVTTLDALDDTIEMLSALNTGSRGNKARRGRIDLAALLVEIAPSARIAIEPGAGTE
                     VFGEESELRRMLNLLVNQAAAGATETEVRIRRLGDLIKISVDLGPDAAQAGELERRWL
                     SRMATRHGGSFELDGATQSILLQADGASDQREVHELRKELEQAQQLGEAYARELAAVL
                     GSGDVRSEPPPSVASTDGASRLEVLRGAFSAFERICRSTAEAARTDAAAVAKLDGGAD
                     VYQSLTRR"
BASE COUNT          487 a          947 c          850 g          420 t
ORIGIN      
        1 accgggcttc cccgctggct ctcggccacg actcgatact cccctgcggg aacctcgaac
       61 gcacgcgcgc ggaccggaga ttcggcgcca ctctcgcacg cccacgccgg gcgacttcga
      121 cattcccgat ccaggagcgc caccgcgccg gtatcaccgg aagacaagcg ttcgaccagc
      181 agcacgtccg atgtctctgt tagaggcaag cttcgagtgg tatccaccgc gcccgcaaga
      241 cactccactg cgaccgagct cgtgcccttc gcgaactcga cgatctcggt ggagccgggc
      301 gtaatggcgg gggcctcgga gcagagctcg ccgggttccg gcgccgttgc cggttgcacg
      361 tcgaaatgca gttcgacatc ccccggtccc gtcgagccga cggccagaaa gtactcgccg
      421 ggctcgagcg cccgttcgaa cagccgcgcc gggctgccca cacgacagtc gagttcatcc
      481 gtttcgtccg cgcaggccgc gcgtcgcagg gacagcgtcg gactacccaa atcatccaaa
      541 ggccaggccg aagcgatgac gtcgctctgc gactccaact cgaagcgata gagcagctcc
      601 gagtagctct ccgcgcaggc gccgctgacg tcgcgcgctg cctccgcgag agaggccact
      661 gcccgcgccc ccgattcgat cgatgcggcc gcaccgcagg tctcgttttc cggcgcgggc
      721 ttcgcctcgc catagtggac acgaagctcg agcgggctct cgctcgctcc gctcacgaag
      781 acggggtaaa caccaggttc gaacccgcgc aaccaaaggc gtgcggcgaa ggactgcgat
      841 tcgagtgccg ccgcgcgagc acacgacagc tcgctccctt tgcggctgca agaggccgcg
      901 atggctaggg caagctccgc gtccgcggcc agcaccgcga cgtcgacgtc gaccgcttcg
      961 ccctcgggaa tgcgaatggc cgccaccacg tcgcggcgcg cggggaccga ccgcgcgcag
     1021 ctcagaccgt agtcctcgcc ggcaccctcc aggttcaaac catacgtgcc actcgcttcg
     1081 atcacgagtg catcgacaca cgtgtcgtga gctggcgtcg tgcaatcgcc ctcgtccgac
     1141 tctccatcgc agtcgtcgtc gcgctggttc gcgcagacct cgcgagtcag actgcttacc
     1201 ttgggatcgg tgtcgtcgca gtcgccgccg ccagcgcagt tccaaacccc gtccccgtcc
     1261 ccgtcggcgt cccgtaccaa gtgttcgcag ctccgcgtct cttcgataca gcgatcgatg
     1321 gtgcaggtgg tgccatcatc acaggctacg ctcccgccgg gacggcaacc gattccaggt
     1381 aaacactgct cgataccgtc acagaacacg tcgttgtcac agcgcgtatc gactggcgaa
     1441 aatcgacagc gacccgacgc cgcatcacaa ctatcctcgg tgcactcgag tccgtcatca
     1501 cactgggcag attcctggca aacacctccc cactcgttcg gaccaccacc ggtcgagaga
     1561 cgaccgcccg tgcttggagc ggcgcctgct cctgcttcac cgccactacc cgcttgtagg
     1621 tcatcgaagg ggctgggtgc cgtagcgtca ctacacgcaa caacacacag tccggcaacc
     1681 caacaggcga gcccaagcaa gcgcatgcgc gggttatagc ccacggcgcg ccgttcgggg
     1741 tatgggcggt gtcgcactgt atcagcccgt atcagcgaca ctcatacttt cgcgcgccgc
     1801 ggttcgtcgc agggctagag attcgagagc cacccgcgta acatgcctgt cgtgtcgaac
     1861 gaacgactta cacgacacga gatcacgtgg cttttggcgc aggaggcacg cggcgcggcc
     1921 cgcactctgc gttcggaggt gggaagagtt tcaatgccga gcgccgtgcc ggcgcccggc
     1981 accgaaccgg tcgtgacgac cctggacgcg ctggacgata caatcgagat gctgagcgcc
     2041 ctgaataccg gctctcgcgg caacaaggcg cgtcggggac gcatcgattt ggccgcgctg
     2101 ctggtcgaaa tcgcgccgag cgctcgcatc gccatcgagc ccggcgctgg caccgaggtg
     2161 tttggggaag aatcggagct gcgtcgcatg ctgaacctgc tggtgaacca agcggcggca
     2221 ggtgcgaccg aaacggaagt gcgcattcgc aggctgggag acctgatcaa aataagcgtg
     2281 gatctcggac cagacgcggc ccaggcgggc gagctcgaga ggcgctggct gagccgtatg
     2341 gcgacgcgtc acggcggctc gttcgagctc gacggcgcga ctcagtcgat cctgcttcag
     2401 gcagacggcg cgagcgatca gcgcgaagtg cacgagctcc gcaaagaact cgagcaagct
     2461 cagcagctcg gtgaagcgta cgcgcgcgag ctcgccgccg tgctcggttc cggcgatgtc
     2521 cggagcgagc cgcctccttc ggtcgcgagc accgacggcg cgagccgctt ggaggttctg
     2581 cgcggagcct tctctgcctt cgagcgcatc tgccgaagca ccgccgaagc agcgcgcacg
     2641 gacgccgccg cggtcgcgaa gctcgacggc ggcgcggacg tctaccagtc gttgacgcgc
     2701 cgcg
//
LOCUS       DASTKS010000436         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_68971,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000436 DASTKS010000000
VERSION     DASTKS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68971"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFQ35_09100"
     CDS             complement(<1..236)
                     /locus_tag="VFQ35_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930831.1"
                     /translation="MRSKAQAKRDARRARVEALKSIGAFVVLAKPHRSLLDEGNAIAR
                     NARAEVAERSAEIRDRRGLTGADHGARDAARDEL"
     gene            complement(293..508)
                     /locus_tag="VFQ35_09105"
     CDS             complement(293..508)
                     /locus_tag="VFQ35_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930832.1"
                     /translation="MAHHVDARLRDVPPEILDVLTRELEQRSPAAVAREAGVSRSVVL
                     AAAARAGVLPGSLSLLREFARTRGTTA"
     gene            complement(628..858)
                     /locus_tag="VFQ35_09110"
     CDS             complement(628..858)
                     /locus_tag="VFQ35_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930833.1"
                     /translation="MSDDMAHEFHDPRTRPVPDDLRDRARALMRERGPRAAAMALGIS
                     RTALLAICGGADVLPGTLALVREAERAGGRIA"
     gene            complement(1027..1926)
                     /locus_tag="VFQ35_09115"
     CDS             complement(1027..1926)
                     /locus_tag="VFQ35_09115"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9930834.1"
                     /translation="MSGDRFDLVAAQRRIFESTLGPLERLVALAILDHWSRAHAKPYP
                     SVARLEAWTGLSRNAVLNAIRGLERAGAIGVTRGFGISSTYDLGPLMRLPVRDVNRFT
                     GGTGSPDEPVHQKDPTSSPEVPPPVHQKDTKEPREGTKRRNQGSRTGVRKGSTPVQVS
                     LLSEPAEPEPAEPEVASSDHRIVTECYFECFERERGQRPVFRSIEGKAVKDLLTAAGS
                     AERACEFIRRAFASFRRKTVTIQQIAKDPSAFAVEARQGACAPQETPDGARFDDEAER
                     RAEELASRGGATSKASSACRARE"
     gene            complement(1916..2455)
                     /locus_tag="VFQ35_09120"
     CDS             complement(1916..2455)
                     /locus_tag="VFQ35_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930835.1"
                     /translation="MWDYARFSDEAWRARCHPKILNVLEQWDFRQSMLVLGPTGSGKS
                     SGIVARLYRELERLTALVQETGEAHTFDFAYTTGLDLSGCRRRSKIGAEATLISHLAS
                     KRLIIIDEVGFEPPGEELLAVIDRHYIGGGTIVVCSGLTLEAFDARYGGALRRRIVER
                     GMLLNGHPARNKGQVRSVG"
     gene            complement(2698..2907)
                     /locus_tag="VFQ35_09125"
     CDS             complement(2698..2907)
                     /locus_tag="VFQ35_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930836.1"
                     /translation="MMEMTMSLTGEDLDAIDRAPARDDLYCVVCALEWWACACDPSHP
                     ERRPTPEEIEDAREQRLSRAERGGR"
     gene            complement(2904..3134)
                     /locus_tag="VFQ35_09130"
     CDS             complement(2904..3134)
                     /locus_tag="VFQ35_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9930837.1"
                     /translation="MTTEIDWVAEVIAGLPALVTVKEAVAALRMSRRHFYRLVARGQI
                     KTVQQSTGGSSRHLIARGEIARYLRSLDARAA"
     gene            complement(3228..3815)
                     /locus_tag="VFQ35_09135"
     CDS             complement(3228..3815)
                     /locus_tag="VFQ35_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930838.1"
                     /translation="MNGKPMTGRERAALLQRFEADLAAIDSENLARLLLAEFDRRRLD
                     EAGNPTDAPGLYRAAQTSMRALLGAIDEADPADVRLARNLAAVGRGVETRSKEGEDAP
                     LIREIASFVETMMTSKTVAQEERAGRMAKTFSKRLKRNVDSSEIDKALRFTFPRRITE
                     VALACGVFKKDATLGAAYWRKPDQRVRSALGLKKK"
     gene            complement(3889..4302)
                     /locus_tag="VFQ35_09140"
     CDS             complement(3889..4302)
                     /locus_tag="VFQ35_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9930839.1"
                     /translation="MKCHECNGEKFERRVVELSTQVGDQVVVDRSVRLPVCMHCDAFV
                     VPSSVLEMVELRAALVAFLDAPRVTGEMLRFARKALGSTQTELAARLSVSMESVSRWE
                     REERPMEPWVPLAVLGLVRERLMPAPENVELKKTA"
     gene            complement(4313..4573)
                     /locus_tag="VFQ35_09145"
     CDS             complement(4313..4573)
                     /locus_tag="VFQ35_09145"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HET9930840.1"
                     /translation="MTPSEALQQAALAGKTGQFDVSSHCLKRMRERRVTRYDIRLALQ
                     SATGATHQDGDKWRLEGGRDDGGDELGVVVVFTGHTLVVTVF"
BASE COUNT          706 a         1642 c         1516 g          855 t
ORIGIN      
        1 tcgagctcgt cgcgggcggc gtcgcgtgct ccgtggtcgg cgcccgtgag gccgcggcga
       61 tcgcgaatct cggcggagcg ctccgcgacc tcggcgcgcg catttcgagc gatggcgttg
      121 ccctcgtcga gcagcgagcg atgcggcttc gcgagcacca caaacgcgcc gatcgacttc
      181 agcgcctcga cccgcgcgcg gcgggcgtct cgcttggcct gagctttcga acgcaccggc
      241 gccgcgctgc ggctggccga ggtgtcccat cggccggcgg tagctgcggc gctcacgcgg
      301 tagtcccccg cgtccgcgcg aactcgcgga gcaggctcaa cgagcctgga agcacgcccg
      361 cgcgcgctgc ggccgcgagc accacgctgc ggcttacgcc tgcttcgcgg gcgacggcgg
      421 ccggcgagcg ctgttcgagc tcgcgggtga gcacgtcgag gatctcgggg ggtacgtcgc
      481 gcaaacgggc gtctacgtga tgagccatgt gccagtgatg atgaatcacg tccttgcggg
      541 cgtcagtgga gcctgtgact ccacctgggc ggctcgcctt tccgggggcg gaggctcgaa
      601 gcgctccgcg cgtattcgcc gtggccgtca cgcgatgcgc cctccagcgc gttcggcctc
      661 gcgtacgagc gccagagtgc ccggcagaac atccgcgcct ccgcagatgg ccaggagcgc
      721 cgtgcggctg atgccgagag ccatcgcggc cgctcgcggg ccgcgttcgc gcatcagcgc
      781 gcgggcacga tcgcgcaggt cgtcgggcac gggacgggtc cgggggtcgt ggaattcgtg
      841 ggccatgtcg tcgctcatga gtaaagcgtc acgagcccgg cgcgtgtgtg acggatgggg
      901 tggtgtcaca cgcggcggac tcttcccgaa tcgctgctcg ctcgtcgggg tcgtcgaagg
      961 gcctcggggc accggagctc gatggtgcca ttcgggcagt tgagcgcgta cgcgtcggag
     1021 tcgagctcac tcacgagctc ggcatgccga gctcgctttg ctggtcgcgc cgccgcgcga
     1081 agcaagctcc tccgcacgtc gctccgcctc gtcgtcgaaa cgcgcgccat cgggcgtctc
     1141 ctggggcgcg catgccccct ggcgggcctc tacggcgaac gcgctcgggt ccttcgctat
     1201 ctgctggatc gtgacggtct tgcgtcgaaa gctcgcgaag gcccgcctga tgaactcgca
     1261 tgcgcgttcg gcgctgccgg ccgccgtgag gaggtccttc acggctttgc cctcgatgct
     1321 ccggaatacc ggtcgttggc cgcgctctcg ttcgaagcac tcgaagtagc actcggtgac
     1381 gatccgatgg tccgagctgg ccacctccgg ctctgccggc tccggctctg ccggctccga
     1441 gagcaacgat acctgaaccg gcgtggaacc cttccgcacg ccagtgcggg atccttggtt
     1501 ccttctcttg gttccttctc ttggttcctt cgtgtccttc tggtgaactg gtggcggtac
     1561 ctctggtgaa ctggtggggt ccttctggtg aactggttca tcaggcgaac cggtacctcc
     1621 ggtgaaccgg ttcacgtcgc gcaccggtaa gcgcatcaaa ggcccgaggt cgtaggtgct
     1681 ggagatgccg aacccgcgcg tcacgccgat cgctcctgct cgttcgagcc cgcgaatcgc
     1741 gttgagaacc gcgttccggc tgagcccagt ccatgcttcg agccgcgcga cgctcgggta
     1801 tggcttggcg tgtgcgcgcg accagtgatc gagaatggcg agcgcaacga gccgctcgag
     1861 aggcccgagc gtgctctcga agatccggcg ctgcgcggcg acgagatcga agcggtcacc
     1921 cgacactccg cacctggccc ttgttgcggg ctgggtgccc attgagtagc attccgcgct
     1981 caacgatccg tcgccgtagc gctccgccgt agcgcgcgtc gaacgcctcg agcgtcaggc
     2041 ccgagcagac gacgatcgtc ccgccgccga tgtagtgccg atcgatcaca gccaagagct
     2101 cctcccccgg cggctcgaat ccgacctcgt cgatgatgat cagacgcttg ctcgcgaggt
     2161 gcgaaatcag cgtcgcttcc gcgccgattt tcgagcggcg gcggcagccc gaaaggtcga
     2221 ggcccgtcgt gtatgcaaag tcgaacgtgt gcgcctcgcc cgtctcctgg acgagtgcgg
     2281 tgagacgttc gagctcgcga tacaggcgcg ccacaatgcc gctcgatttg ccgctcccgg
     2341 tcggcccgag caccagcatc gactgccgga aatcccactg ctcaagtacg ttgagaattt
     2401 tcgggtggca tcgcgcgcgc cacgcctcgt cgctgaaccg ggcgtagtcc cacatcggcg
     2461 tatgaagtag cggctggcca cattcgatcg tctggccagt aaggcagtgg tcgaagctcg
     2521 ctcggagccg cgagagattc agctcgcgct catgctcaaa ccgacgccgg tccgcggcac
     2581 gaatcttttc gcgccagggg tcgaggcacc tctcgtgcac gaccatccgg ctgtcccgcg
     2641 accgccgcgc cggctcgtca ggctgcactg ggtcggcgca atacgcgcag aggtcgctca
     2701 tcgcccacct cgctcggcgc gggacagccg ctgctcgcga gcgtcctcga tctcctcagg
     2761 tgtcggacgc cgctcggggt gcgacgggtc gcacgcgcac gcccaccact cgagcgcgca
     2821 cacgacgcag tacagatcgt cgcgcgcagg cgcccgatcg atcgcgtcga ggtcctcgcc
     2881 ggtgaggctc atagtcatct ccatcacgcc gctctcgcgt cgaggctgcg caggtagcga
     2941 gcaatctcgc cgcgcgcgat cagatgccgg gagctgccgc cggtagactg ctggacagtc
     3001 ttgatttgac ctcgtgcaac gagacggtag aagtggcggc ggctcatccg gagggccgcg
     3061 accgcttcct tcacggttac cagcgcgggg aggcccgcga tcacctcggc gacccaatcg
     3121 atttcggttg tcatgagaga gaaccctttc cgagcgggcg tgtgccgccc tgggtcccgg
     3181 tgtgacaact gccgccggtt cgagcagtag cccctaagac tccactctca ctttttcttc
     3241 agaccgagcg cggatcgcac cctttggtcc ggcttccgcc aatacgcagc gcccaacgtc
     3301 gcgtccttct tgaacacgcc gcaagcgagt gcgacctcgg tgatcctgcg cggaaacgtg
     3361 aatcgtagcg ctttgtcaat ctcagacgaa tcgacgttgc gcttcagtcg cttcgagaac
     3421 gtcttcgcca tcctgccggc gcgctcctcc tgcgcgacgg tcttggacgt catcatcgtc
     3481 tccacaaagg atgcaatctc gcggatgagg ggcgcgtcct caccttcctt cgagcgcgtc
     3541 tctacgccac gaccgacggc cgcgaggttc cttgcgagcc tcacgtccgc tgggtcggcc
     3601 tcatcgatcg ctccgaggag cgcgcgcatc gaggtctgcg cagcccgata gagaccaggt
     3661 gcgtcggtag gattcccggc ctcatcgagg cgccggcggt cgaactctgc gagcagcagg
     3721 cgcgcaagat tttctgaatc aatcgcggcg aggtcggcct cgaaacgctg gagcagcgct
     3781 gcgcgctcgc gcccggtcat gggcttccca ttcatcccgc tttcttctcc ctcgtcgccg
     3841 gccgctcgct caccgcgtcg atcgcgtgct gcatctgctc acgctcgcct atgccgtctt
     3901 cttcagctcg acgttctccg gcgccggcat caaccgctca cgcacgaggc caagcaccgc
     3961 gagcggcacc cacggttcca tcggccgctc ttcgcgctcc cagcgcgaca cgctctccat
     4021 cgacaccgag agccgcgccg cgagctccgt ctgcgtcgat ccgagtgcct tgcgcgcgaa
     4081 ccgcagcatc tcgcccgtaa cacgcggggc atcgagaaag gcgacgagcg ctgcgcggag
     4141 ctccaccatt tccagaacgc tcgagggaac gacgaacgca tcgcaatgca tgcacaccgg
     4201 cagccgcacc gaccgatcga cgacaacctg atcgccgacc tgtgtggaga gctctacgac
     4261 gcggcgctcg aacttctcac cgttgcactc gtgacatttc atggttcgct cctcagaaca
     4321 ccgtgacgac cagcgtgtgg cccgtgaaca cgacaacgac gccgagctcg tcgccgccgt
     4381 cgtcgcgccc gccttcgagg cgccacttgt cgccgtcttg atgcgtcgcg ccggttgccg
     4441 actgcagcgc gagacggatg tcgtagcgag tcactcgtcg ttctctcatt cgcttcaggc
     4501 agtgcgaact cacgtcgaac tgccccgtct tgccggcgag cgccgcctgc tggagcgctt
     4561 cgctgggagt catcgtgtta ctgctccacc ctgggccctg gttgacctgg cgtcaagtgt
     4621 cgctggcggt cggcttgacc ctattctgtt gataactttc accatttgcg gctctatctg
     4681 catcgccgcc tcgagcgtcc gcgcctccct cgagatgcc
//
LOCUS       DASTKS010000437         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1408341, whole genome shotgun sequence.
ACCESSION   DASTKS010000437 DASTKS010000000
VERSION     DASTKS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408341"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFQ35_09150"
     CDS             <1..430
                     /locus_tag="VFQ35_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930841.1"
                     /translation="AVVENVHSQVAIALTHFGARVSEGKLTVIGAVYDFRNDLGKGSG
                     KVTIVDVNGNAEPARMDAFVEAIESDGVIKSKKDKDPRGKRRETGLPTSAADIISPEL
                     QKAIERANEIGGKRAAAAESPAPAAPAAHAAPAAHAGHEH"
     gene            complement(842..1189)
                     /locus_tag="VFQ35_09155"
     CDS             complement(842..1189)
                     /locus_tag="VFQ35_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930842.1"
                     /translation="MRRTTPHVPGDKGSAPLPAIPAPPPLPLAPPGCALAPVPSPPAP
                     PCCELVPPAFAPLAEVAAPEPAALAPPLLAAVPPAPSETVPLTPLQPPELAALAPPEV
                     DLASVPPWPTPPL"
     gene            1179..2171
                     /locus_tag="VFQ35_09160"
     CDS             1179..2171
                     /locus_tag="VFQ35_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930843.1"
                     /translation="MRRISLANAGASTSTFRQSTAYDRVRRVNGAFVAKPVMNAMDPV
                     LLQISWLRIEPDGSQATLAAQSDKVVDVYDLVSFGSPRRIFHVGTDYGNHYVAAFGDD
                     RTSKLTKGSPLFDVQANADFRYVEHGVAVSFDGERALVMHDVLSAYGPRLALIGADAN
                     RVGEMVQLPISSNCQTLRPTAHGGAYHSSDGGLHWVELGSAGDVVLDVRLSFEASDRL
                     ACPLVAATEQGFAAFTFVPFMGWSIIRVTSDGTQSTQVWDALTGTPSAIAVTGDSAIA
                     IVEDSEGKRIAWLKNGKTQYFPIDGKGEQIPSEAGTLFMYWPATREIVEVRCSG"
     gene            complement(2289..>3010)
                     /locus_tag="VFQ35_09165"
     CDS             complement(2289..>3010)
                     /locus_tag="VFQ35_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930844.1"
                     /translation="QPTLVSPNDRACGAARYARQQTPNGVRIYELAPAPARFRQELRD
                     PLPDPPIDIFRCTAVQSAGEGFVLGKDVTGSFPKTSTISVGSGAAHVELSRIGTSTAS
                     ASRPSALLPRQEPDEFVLGDGRHCRVREATDGTLRCAVLEGNAYQNGFVDPECKVRLY
                     ELSSEVLEDAVAHPSWLGVSDYDSRGKLTGSYSLERYTGDTYEFSSGTCLPADSSSSN
                     RWVANQRTDFTEMPLVELRQL"
BASE COUNT          541 a          903 c         1063 g          503 t
ORIGIN      
        1 ggccgtcgtc gagaacgtgc actcgcaggt tgcgattgcg ctcacccatt tcggcgcacg
       61 cgtgtccgaa ggcaagctca ccgtcatcgg cgccgtttac gacttccgca acgacctcgg
      121 taaaggctcg ggcaaggtga ccatcgtcga cgtgaacggc aacgccgagc cagcgcgcat
      181 ggacgctttc gtcgaagcca tcgagtccga cggcgtcatc aagagcaaga aggacaagga
      241 cccgcgcggc aaacgacgcg agaccggttt accgacgagc gccgcggaca tcatcagccc
      301 cgagcttcaa aaagcgatcg agcgtgcgaa cgagatcggc ggcaagcgcg cggccgcggc
      361 tgagtcgcct gcacctgctg ccccggctgc tcatgcggcg ccggctgctc acgccggtca
      421 cgagcactga gacccagtcg aaatcttcaa gggttggtgg acgttgcggc gctcgtaact
      481 ttgtagtgcc accagattct cgcgccgggt gtcgagcggt gtgtcgcggc cgagggtcca
      541 gcgaacgcag attgtcgtgc gccgagctcg catcgagacg tcgtgagcgg gcattgatcc
      601 cgtagacgag cctgcgccgc gagctccggg cggctagcgc gacgttggtt cgagagtccg
      661 tatgggaggc gaacgtgggc agaggaagtt caggttctgc gggtgggaga acgccgagga
      721 cgcgctgggg agcgctggcc ggctccgcct tgctcgttgc gaccgcgtgt gggcagagct
      781 catcgaactc gaaggactcc gcgggctcgt ccggggcaag cgattccgga gggggtccga
      841 atcagagcgg cggagtaggc cagggtggaa cggaggcgag gtcgacttca ggcggagcga
      901 gcgctgcgag ttcggggggt tggagcggag tcagcggaac cgtttcgctc ggcgctggag
      961 gcacggccgc gagcagcggt ggcgccaacg cagccggttc gggagcggca acctcggcga
     1021 gcggcgcgaa cgcggggggc acgagttcgc agcaaggtgg cgccggcggg gacggtacgg
     1081 gcgcgagcgc gcaacccggc ggagcgagcg gaagcggtgg cggcgcggga atcgcgggca
     1141 acggagcact gcccttgtct ccgggtacgt gcggcgtcgt gcgtcgcatt tcgctcgcga
     1201 acgctggcgc gtcgacgtcg acgtttcgtc agtcgaccgc gtacgatcgc gtgcgccggg
     1261 tgaacggcgc gttcgtcgcg aagcccgtga tgaacgcgat ggatccggtc cttttgcaga
     1321 tcagctggct gagaatcgag ccagatggat cgcaggcgac gctcgcggcg cagtccgaca
     1381 aagtcgtcga cgtctacgat ctcgtgagct tcggttcgcc gcgtcgcatt tttcacgtgg
     1441 gtaccgacta cgggaatcat tacgtcgccg cgttcggcga tgatcggacc agcaagctca
     1501 ccaagggttc gccgttgttc gacgtgcaag ccaacgcgga cttccgctac gtcgagcacg
     1561 gcgtggcagt ttccttcgat ggcgagcgcg cgctcgtcat gcacgacgtg ctcagcgcat
     1621 acgggccgcg cttggccttg atcggcgcgg acgcgaatcg cgtgggcgag atggtccagc
     1681 taccgatctc gtccaactgt caaacgctga ggcccaccgc gcacggcggc gcgtatcact
     1741 cgagcgacgg gggactgcac tgggtcgaat tgggctccgc gggcgacgtc gttctagacg
     1801 tgcggctttc gttcgaagcc tccgaccgtc tcgcgtgccc gttggtcgcg gcaaccgagc
     1861 agggatttgc ggcgttcacg ttcgttccgt tcatgggctg gagcatcatt cgggtcacga
     1921 gcgacgggac ccagagcacg caagtgtggg acgcgctaac gggtacgccg agcgccatcg
     1981 cggtcacggg cgactcggcg atcgcgatcg tcgaagactc ggaagggaag cgtattgcgt
     2041 ggctcaaaaa tgggaagacc cagtactttc caatcgatgg gaagggcgaa cagatccctt
     2101 cggaggcggg gacgcttttc atgtactggc cggcgacgcg cgagatcgtc gaggttcgct
     2161 gctcgggcta agagccaatc ctaaatccct cccgtcgccc ggcgtcagat ccgcttcgca
     2221 ttccttcgtt gcttctaaga gccaatcctg aatctgatct aggactggct ctcagcgcgt
     2281 tcgaaatgtc agagctgacg gagctcgacg agcggcatct cggtaaaatc ggtgcgttgg
     2341 ttggcaaccc aacggttcga ggaagaggag tcagccggga gacaagtgcc ggaggagaac
     2401 tcgtacgtgt cgccagtgta gcgctcgagt gaatacgagc cggtgagctt cccgcgcgaa
     2461 tcgtagtcgc tgacaccgag ccagctcgga tgggccacgg cgtcttcgag gacctcgctc
     2521 gagagctcgt aaaggcgcac cttgcattcc ggatcgacga agccattctg gtaagcgttc
     2581 ccctccaaca ccgcgcaacg cagcgtgccg tcggttgcct cgcgaacgcg gcaatgacga
     2641 ccatcgccga gcacgaactc gtccggctct tgtcgaggga gcagcgcgct cgggcgtgac
     2701 gcgctcgccg tggacgtgcc gatacgagaa agctcgacgt gcgcggcgcc gcttcccacc
     2761 gatatcgtcg aagtcttcgg gaacgagccg gtgacgtcct tgccgagcac gaagccttcc
     2821 cccgcggatt gcacggcagt gcagcgaaaa atgtcgatgg gcggatccgg cagcgggtca
     2881 cgcaactctt ggcggaagcg cgccggagcg ggagcgagct cgtaaatgcg aacaccgttc
     2941 ggggtctgct ggcgagcgta gcgagcggct ccgcacgcgc ggtcgttggg cgaaaccagg
     3001 gttggctgcg
//
LOCUS       DASTKS010000438         6728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1854756, whole genome shotgun sequence.
ACCESSION   DASTKS010000438 DASTKS010000000
VERSION     DASTKS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854756"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..957)
                     /locus_tag="VFQ35_09170"
     CDS             complement(4..957)
                     /locus_tag="VFQ35_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930845.1"
                     /translation="MNGVSTSVASWSALAICAAASTAAAQGAPKAVTQEPARLEDTGC
                     VGTDPERTNELFSRAISAWQQGDTAGAVGAMRSAHAISKCSAFLFVLGEMLRQSESDC
                     DALVWYERYVGEGPNVEQRERALDSIAELRARCPASPASTEEKQSRAGMVEAAPAAAA
                     PGSAAPSPEPARLASVSPGSTERTRVRFGDASHRTEARGYWSPAHIGGWASLGASALL
                     GSGAIYFAMRARSASSDYEQLWQSAVGDPAQRDEWRRQRAELEERGSSSATSARVLGG
                     AAGALAVGGVLLLIFDRSGRNQDVALSAGRSAMMARYGVRF"
     gene            1288..2034
                     /locus_tag="VFQ35_09175"
     CDS             1288..2034
                     /locus_tag="VFQ35_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930846.1"
                     /translation="MAELREDSLLFSLEALMTRERERVQLERAEAERHRAMAAAAQAA
                     REKERLETALLREQQLEQARIEEQRRMAEHAAKLDALRQAEFVRAQAEASARAETELL
                     AQRHAHERGLAELALQAKQGRDRALALCSGALLALLLPTALLFHFAHTVPRTRELEGE
                     LRKLISVEHARAEVATQQLAKATGRQRELEAELGTLRASLEAHPEAVGPKPTRPANNT
                     PKPVLAASTKGKPSHGPCRDSGDPLDPCLH"
     gene            2227..2709
                     /locus_tag="VFQ35_09180"
     CDS             2227..2709
                     /locus_tag="VFQ35_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930847.1"
                     /translation="MLDLFERELKAVRFGDLDSTRLLGLADEVREAAEALAAHEARTA
                     ELRSALAERQEILLQQAQRALAFARIHAEDDAALTAKLGEISLPRAPKRPKADAPRAP
                     EPSSAPSHAAETAPAESATTEEPAPVEAPAPPARRGKRREAARTVARDDESFDAPAEA
                     "
     gene            2840..3835
                     /locus_tag="VFQ35_09185"
     CDS             2840..3835
                     /locus_tag="VFQ35_09185"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HET9930848.1"
                     /translation="MMKARSAALVGAQIEAFMAEEQESTSTPRRVFAAGLAGAAVTAA
                     LSASSGEPSATGAHEGGPGAASRTRVPVAFLPHGGGPWPFVDVGIGRPDELAALAAYL
                     KSVATLPRQEPKALLVVSAHWEEPVPTVMTSPQPPMLYDYYGFPPESYRITWPAPGAP
                     ELAVRVRALLEQAGFQTASNAERGFDHGTFVPLKLTYPEPRIPTLQLSLKRGLDPAEH
                     LAMGKALAPLRDEGVFIVGSGMTYHNLRAFGPGAAPVSEAFDSWLRDTATAEPKEREQ
                     RLLAWTRAPSARSAHPREEHLLPLMVVAGAALGDRGATAYSGTILGLRISAYHFG"
     gene            4287..5888
                     /locus_tag="VFQ35_09190"
     CDS             4287..5888
                     /locus_tag="VFQ35_09190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930849.1"
                     /translation="MSSTVEAPSAPRNTEEGRALLQARLSQFGRVGMGLSATFLAIAV
                     AITLALRLPGGVATIFSQIGWFGISFVVWAITRRGVLSTRALLAVDVIATQIHCAVGI
                     ALGWGLPIYARPELVQLVFINDLLAIRAFLVPSTSLRTALLGVFPTVGVVVSMLFQYD
                     GRTLAVGAPSARSLAVVAATLGFATVVITTLTSRTIFSLRERVRAALQLGQYTLLEKL
                     GEGGMGVVYKASHAMLKRPTAIKVLPPERAGQHNLARFEREVQLTSMLTHPNTVSIYD
                     YGRTPDGCFYYAMEYLDGVDLETLIAIDGPQEPARVVHILSQVCGALSEAHGIGLVHR
                     DVKPANVLVCVRGGVADLAKIVDFGLVKSLQGGALDATHTAVDQIVGTPLYMAPEAMT
                     SPDSLGPRSDLYALGAVAYYLLTGSPPFTGQSIVEICAHHLHTSPRPVSEQSPKKVPE
                     ALERVVMACLAKRPEDRPESADALARWLAACDIPAWSNEQARAWWETHADAVQRRRAE
                     QSQSLGRPELERATVAIEFASPNAR"
     gene            complement(5905..>6728)
                     /locus_tag="VFQ35_09195"
     CDS             complement(5905..>6728)
                     /locus_tag="VFQ35_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930850.1"
                     /translation="DVLCQQAGPGGGNGGCGPGKCENYCALFPKVCPDESMNQDPCVT
                     NCQGLRDDGNLNSVDDHEGDTIECRLVHLSAATTEPATHCQHARLAKPSAYCTDEALA
                     TPHPLSCDDYCKLVSKVCSGDNAVYEDVEQCKAVCKHFPVGEPGDTSEDTLACRKYHT
                     YNAMSAPVTHCAHAGPGGDGHCGDPVTNNCTSYCNLLSEVCPLEYLTTFVDVQGCLDD
                     CATLPDSVSNSGYSVEKAIHPTGAPLPFGCRMVAVSRAAKTPSLCGVVFDPSGCQ"
BASE COUNT         1103 a         2311 c         2177 g         1137 t
ORIGIN      
        1 agctcagaac cgcaccccgt agcgggccat catcgcagag cgccccgccg acaaagctac
       61 gtcttggttg cggccggaac ggtcgaagat gagcagaagc acgcctccaa ccgccagcgc
      121 gcctgcagcg ccaccaagga cccgcgcgct cgtcgcggac gatgaacccc gctcctcgag
      181 ttcggcgcgc tgccgccgcc actcgtcgcg ttgcgcagga tcgcccacgg cggactgcca
      241 gagctgctcg taatcgctgc tcgcgctccg agcccgcatc gcgaagtaga tggccccgct
      301 gccgagcagt gcgctcgcgc cgagcgacgc ccaacccccg atgtgcgcgg ggctccagta
      361 gccgcgcgct tcggtccggt gcgaggcgtc gccgaaccgc acccgtgtgc gctctgtgct
      421 acccggcgaa accgatgcga ggcgggcagg ctctggcgaa ggcgctgcag agcccggagc
      481 ggctgctgcc ggcgccgctt cgaccatacc cgcccggctc tgcttttctt ccgtactcgc
      541 gggcgacgcc ggacagcgtg cccgcagctc cgcaatgctg tccagtgcgc gctcccgctg
      601 ctcgacgttg gggccttccc caacgtagcg ctcgtaccaa accaaggcgt cacaatcact
      661 ttccgactgc ctcagcatct cgcccagcac gaacagaaat gccgagcact tcgagatcgc
      721 atgggcgctg cgcatcgccc caaccgcgcc ggcagtatcg ccttgctgcc acgcgctgat
      781 cgcgcgcgag aagagctcgt tcgtgcgctc tgggtcagtt cccacgcacc cggtgtcttc
      841 gagccgcgcg ggctcctgcg ttacggcctt tggcgcgccc tgcgctgcag ccgtcgaggc
      901 cgccgcgcag atcgccagcg cactccaact cgcaaccgag gtcgacaccc cattcatggt
      961 acgtcgacct cgctcgaagt ttcgataggt gttttttcga aggggctacg ctccgggaag
     1021 tgcgcgcaag gacgtcgcat ctcgcactcg acacctccga gcgcagcgag cgggtccgta
     1081 gccgcgcccg gcgcgcgccg tcgtggacga ccgcgagtcg ttcggcgcat tcagcgagca
     1141 ccgcgagaac ccgtgtccaa gcgtcccctc ccccccggga gcgggcggtg ggcggggggg
     1201 acatcgtcat cggaccaccc aaggagcgcg agccggcgga gtttcacgga gagcgcagac
     1261 tcgaagcgct caaaccggtt ttcaaccatg gcggagctcc gagaagattc cctcctattt
     1321 tcactcgagg cattgatgac gcgagagcgc gagcgggtgc agctcgagcg cgcggaggcg
     1381 gaacgtcacc gagcgatggc cgccgccgca caggcagcgc gagagaagga gcgcctcgag
     1441 actgctctcc tgcgcgagca gcagctcgag caagctcgca tcgaagagca gcggcgaatg
     1501 gccgaacacg cggcgaagct cgatgcactt cggcaagcag agttcgtccg cgcccaggcc
     1561 gaggcgagtg cgcgcgccga aaccgagctg cttgcacaac gacacgctca cgaacggggt
     1621 cttgccgagc tcgccttgca ggcgaagcaa ggccgagacc gagcgctcgc actttgcagc
     1681 ggtgctctgc tcgcgctgct gctaccgacg gcgctcttgt ttcatttcgc gcacacggta
     1741 ccgcgaacgc gtgaactcga aggtgagctc cggaagctaa tctcggtcga acatgcgcga
     1801 gcggaggtag caactcagca gctcgcaaag gccacgggac gtcagcgcga gctcgaagcc
     1861 gaactcggga cgctgcgcgc ctcgctcgaa gcgcacccag aggcagtcgg cccaaagccg
     1921 actcgacccg cgaacaacac gccaaagccc gtgctcgccg cgtccaccaa aggcaagccg
     1981 tcgcacggcc cctgtcgcga cagcggcgac ccgctagacc cgtgtttgca ctagctataa
     2041 cagggggttc ccaagtctct ccgggctgct gcgccaagat cctgcggcta catcagcgcg
     2101 gttgccgcgg gacgacgagg ccgagtacgc cccggaacgc ggcgtttctc acttggggct
     2161 tccgttgcgc gaaacctgaa tgtatgttca gggcgcatgt ctccgatttc cgcccctatc
     2221 caagctgtgt tggacctttt cgagcgtgag ctcaaggccg ttcgcttcgg cgatctggat
     2281 tcgacgcgcc ttttagggct cgccgacgag gtgcgcgagg cggccgaggc cctcgccgcg
     2341 cacgaggcac gcacggccga actgcgctcc gcgctcgccg aaaggcaaga aattctcctg
     2401 cagcaagcgc agcgggcact ggctttcgct cgcatccatg ccgaagacga cgcggcgctg
     2461 accgcgaagt tgggggagat ttcgctgcca cgcgcgccaa agcgacccaa ggccgatgca
     2521 ccgcgggccc cggagccgtc ctcggcgcca tcgcacgccg cggaaaccgc ccccgcagag
     2581 agcgccacaa cggaagagcc cgccccggtc gaagcgccag cgccgcctgc tcgccgcgga
     2641 aagcggcgcg aagccgctcg caccgtcgcg cgcgacgacg agagcttcga cgcccccgcc
     2701 gaggcttgat tccagccgct ccgggtgcgc gggcaccttc gctcgtaatc cgccgctcca
     2761 tcgtcgcgtg atgattggtg cgatttcggg atcagtgtgt tcccgcgctc gcagctttct
     2821 cgttcacgct cgacgacgca tgatgaaggc tcggagcgcc gcgctagtgg gcgcgcagat
     2881 cgaggctttc atggcagaag agcaagaatc gacttcaact ccgcggcgcg tcttcgccgc
     2941 tggcctggcg ggcgcggcag tcacggctgc gttgtccgcc agcagcggag agcccagcgc
     3001 caccggggcg cacgagggtg gcccgggcgc ggcgtcgcga acgcgcgtgc cggtcgcttt
     3061 cctgccccac ggcggagggc catggccgtt cgtcgacgtg ggcattgggc gtcctgacga
     3121 gctcgccgcg ctcgctgcgt acctgaagtc agtcgcgacg ttgccccggc aagagccgaa
     3181 ggccctgttg gtcgtttccg cccattggga ggaaccggtc ccgacggtga tgacttcgcc
     3241 gcagcctcca atgctctacg actactacgg cttcccgccc gagtcgtatc gcatcacctg
     3301 gccggcgccc ggcgcaccag agctcgctgt gcgcgtgcga gcgctgctcg agcaagctgg
     3361 gtttcaaacc gcgagcaatg cggagcgcgg gttcgaccac ggtaccttcg tcccgttgaa
     3421 gctcacgtac cccgagccgc gcatcccgac tctgcagctc tcgctgaagc gcggtctcga
     3481 ccccgccgag cacctcgcca tgggaaaggc actcgcccct ctgagagacg aaggcgtctt
     3541 catcgtcgga agcggcatga cgtatcataa cctccgcgcc ttcggacccg gcgctgcgcc
     3601 cgtatcagag gcattcgact cttggctgcg cgacaccgcg acggccgagc cgaaggagcg
     3661 tgagcaacgg ctgctcgctt ggactcgagc gccctcagcg cgcagcgctc acccgcgtga
     3721 agagcacctg cttccgctca tggtcgttgc gggtgcggcg ctcggcgatc gcggcgcgac
     3781 ggcatacagc ggaaccatcc tcgggctgcg aatttcggcg tatcactttg ggtgaagagt
     3841 gaaccccctc gcgctttgcg agcgggcgag ggctgagtgg aggtttcagc ccttctgcgg
     3901 tgcccacgct tctcgctcgc gcttttcgct cgcgcttcgt ggctctcgtg gccggcagaa
     3961 acttgagagc cccccctagc catccgccgt cctcggcaca acataggagg ggtctcctcg
     4021 aagaagcgga gctttttcat gctctcgatg cgctcgttgc tctcgtccag cccgtttttg
     4081 tcgctcgaaa ctcgtcgtgc cgtggttgcc cgctcgcgcg aagcgcgtca ggcgctggta
     4141 tttctcggcg tgaacgagtg cgaagcgcgc gaactgctcg acgagaagtc agattgttcg
     4201 actcgcagac cggccggaga ggcctcgact cgtctttagg ccgggccgcg cgggctcggg
     4261 cgggcgtctg atatgctgcc gcccgcatgt cgtcgacggt ggaggccccg agcgctccgc
     4321 gcaacaccga agagggccgc gcgctcctgc aagcccggct ctcgcagttc ggccgcgtcg
     4381 ggatggggtt gtctgcgacg ttcctcgcga tcgcggtagc catcacgttg gcgctgcgcc
     4441 tgcccggagg cgtcgcgacg atcttttcgc agattgggtg gtttggcatt tccttcgtgg
     4501 tgtgggccat cacccgacgc ggagtgctga gcaccagggc cctgctcgct gtcgatgtga
     4561 tcgcgacgca gattcactgc gcggtcggca tagcgctcgg ttggggcttg ccgatttacg
     4621 cgcggcccga gttggttcag ctggtgttca tcaacgacct gctcgcgatc cgagcgttct
     4681 tggtgccgag tacgtcgctc cggaccgctt tgctcggcgt tttcccgacc gtcggagtcg
     4741 tggtgtcgat gttgtttcag tacgacggac gcacactcgc ggtcggcgcg ccgagcgcca
     4801 ggtcactcgc ggtcgtcgcc gcgacgctcg gcttcgcgac ggtggtcatc acgacgctga
     4861 cgtcgcgcac catcttcagc ctgcgcgaac gcgtgcgagc ggcgcttcag ctcgggcagt
     4921 acacactgct cgagaagctc ggcgaaggcg gcatgggtgt cgtttacaag gcaagccacg
     4981 ccatgttgaa gcggccgacc gcgatcaagg tgctgcctcc ggagcgcgcc ggccagcaca
     5041 acctcgctcg cttcgagcgc gaggtgcagc tgacgagcat gctcacgcat ccgaatacgg
     5101 tctcgattta cgattacggt cgtaccccgg acggctgctt ctactacgcc atggagtacc
     5161 tcgacggggt cgatctcgag acgctcatcg ctatcgatgg cccgcaagaa ccagcgcgcg
     5221 tcgtgcacat cctatcgcag gtttgtggcg cgctctccga agcgcacggg atcggcctgg
     5281 ttcaccgcga cgtgaagccc gccaacgtac tcgtctgcgt gcggggcggc gtcgccgatc
     5341 tcgctaagat cgtggacttt gggctcgtga agtcgcttca aggcggggcg ctcgacgcga
     5401 cccataccgc cgtcgatcag atcgtcggta cgccgctcta catggcaccg gaggcgatga
     5461 cgagcccgga ctcgctcggg ccgcgaagcg atctctacgc gctcggcgcc gtagcctact
     5521 acctgctcac gggttcgccg cccttcacgg ggcaatcgat cgtggagatt tgcgcgcatc
     5581 atcttcatac gagcccgaga cccgtctccg aacagtcccc aaagaaggtg cccgaagcgc
     5641 tcgagcgcgt ggtgatggct tgcctcgcca agcgcccgga agatcgcccg gagagcgccg
     5701 atgcgctcgc gcgctggctc gcggcctgcg acatcccggc gtggagcaac gagcaggctc
     5761 gcgcctggtg ggaaacgcac gcagatgcag ttcaacggcg ccgcgccgaa cagagccagt
     5821 cgctcggccg tccggagctc gagcgagcaa ccgtagcaat cgaatttgcg tcgcccaacg
     5881 ctcgctaaaa gctgcgctcg aacgtcactg gcacccgctg gggtcgaaga ccacgccaca
     5941 gagcgacggc gtcttcgccg cgcggctcac cgcgaccatg cggcacccga acggaagtgg
     6001 tgcgccggtc gggtgaatcg ccttctccac cgaatagccg gagttcgaca ccgaatcggg
     6061 aagcgtggcg cagtcgtcca ggcagccttg cacgtcgacg aacgtggtta ggtactcgag
     6121 cggacagact tcgctcaaca gattgcagta cgaagtgcag ttgttcgtca ccgggtcgcc
     6181 gcagtgcccg tcgccgcctg gccccgcgtg agcgcagtga gtgaccggcg ccgacatcgc
     6241 gttataggtg tgatacttgc ggcaggcgag cgtatcctcg ctggtgtcgc caggctctcc
     6301 cacgggaaag tgcttgcaga ccgctttgca ttgctcgacg tcctcgtaca cggcgttgtc
     6361 accgctgcac accttgctca cgagcttgca gtagtcgtcg cagctcagcg gatgcggcgt
     6421 cgcgagcgct tcgtcggtgc agtaagcgct cggcttcgcg agccgcgcat gctggcagtg
     6481 cgtggcaggt tcggtcgtcg ccgcgctcaa gtgaaccaag cggcactcga tggtgtcgcc
     6541 ctcatggtcg tcgaccgagt tcaggttgcc gtcgtcgcgc agaccctgac agttggtcac
     6601 gcacggatct tggttcatcg actcgtccgg gcagaccttc gggaatagag cgcagtagtt
     6661 ctcgcacttg cctggtccgc acccgccgtt gccaccgcct ggccctgcct gctgacacaa
     6721 aacgtccg
//
LOCUS       DASTKS010000439         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1408646, whole genome shotgun sequence.
ACCESSION   DASTKS010000439 DASTKS010000000
VERSION     DASTKS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408646"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1190)
                     /locus_tag="VFQ35_09200"
     CDS             complement(30..1190)
                     /locus_tag="VFQ35_09200"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9930851.1"
                     /translation="MTEPASDRSRHVVVDVSEARIARLWGNVSARLERPRGARRWMMG
                     ALAAGTLAAAAGVAVVHFRSPSELASPSVWEGARLETAADRMSVSLVDGSRLTLDPNS
                     QVAVQDRSTSAVKLVVERGRLACDVTHREGRSFVVVAGGVEVRVVGTLFSVASERAEG
                     AVRVEVNVERGAVEVRAPGESSVARVEAGHSWSQLTRVQTAAASSAPSSAAAPAPSAA
                     EPVPSAGMELSVPAPGASAAALTVAPPSNSRRALQVAPAASSSASPSARELFEQASGL
                     WRDGRPAEAAQAYQNLLSTYPRDARAGLAAFELGRLRMDRLGDLPGALKALERALTTA
                     PGSSFREDALARIVRASSTLGNASRCNSAREQYLREFPNGVHRLSVTKACGL"
     gene            complement(1168..1746)
                     /locus_tag="VFQ35_09205"
     CDS             complement(1168..1746)
                     /locus_tag="VFQ35_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9930852.1"
                     /translation="MTPAAAIPPTKSGAGPSDAALVVAARAGEVWASEALFRRHSRMA
                     LGLAYRLLPRDVEVDDVVQDSFVYALQRLDSLQNPQAFAAWLGSIVVRTVSKRLRRRR
                     LLTRLGLRARESVDPDQVIAASAPGDVRVALRRVYAIVEELPAEQRIALVLRRVDGLE
                     LPQIAETMGLSLSTVKRRLKAAEELLDRTGER"
     gene            complement(1996..>2310)
                     /locus_tag="VFQ35_09210"
     CDS             complement(1996..>2310)
                     /locus_tag="VFQ35_09210"
                     /inference="COORDINATES: protein motif:HMM:NF042873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease superfamily protein"
                     /protein_id="HET9930853.1"
                     /translation="AASGTAFAIECKYQDSVGTVDEKIPYAIQDMQAMGMPVCLAYAG
                     GGFSDGILHMLAACPIAAYCMPPLSLDPCKETRELDVAIAMAFRWWDVAVVNKKPFVP
                     NL"
BASE COUNT          397 a          833 c          752 g          328 t
ORIGIN      
        1 tccggactca cttgctcatt cgcggggccc tagagcccgc aagctttcgt gacgctcagg
       61 cgatgaacgc cgttcgggaa ctcgcgcaag tactgctctc gcgcgctgtt gcaacggctc
      121 gcgtttccaa gcgtcgagga ggcccggacg atgcgcgcca gcgcgtcttc gcgaaagctc
      181 gagccggggg cggtcgtcaa cgcgcgttcg agggctttca gcgccccagg caagtcgccc
      241 aagcgatcca tacggaggcg cccgagctcg aacgccgcga gcccagcgcg cgcgtcgcgc
      301 ggatacgtcg acagcaggtt ctggtacgcc tgcgcagcct cggcggggcg accgtcgcgc
      361 caaagcccgc tcgcctgctc gaagagctca cgcgcactcg gagaagcact gctcgaggca
      421 gccggtgcga cctgaagcgc gcgtcgtgag ttgctcggag gtgcaaccgt gagcgcggcc
      481 gcacttgcac ccggcgccgg aaccgagagc tccatgccgg cgctcgggac gggctccgcc
      541 gcactcggcg caggagccgc tgcgctcgag ggcgcgctcg aagccgccgc cgtttggacc
      601 cgagtcaact gcgaccagct gtgcccggcc tcgactcgag cgaccgagct ctcccccggc
      661 gcccgcactt cgacagcgcc gcgctcgacg ttcacctcga cgcgcacggc tccctcggcg
      721 cgctcgctcg cgacagagaa tagcgtcccg accacgcgca cttcgacccc accggcgacc
      781 accacgaacg aacgaccttc gcgatgcgtc acgtcgcagg caagccgccc gcgctcgacg
      841 acgagcttca cggcggaggt gctgcgatct tgcacggcga cctgcgagtt cggatcgagc
      901 gtcaggcgag agccgtcgac cagcgacacc gacatgcgat ctgcggccgt ttcgaggcgc
      961 gcgccctccc aaaccgaagg gctcgcgagc tcgctgggcg agcggaaatg cacgacagcg
     1021 acgcccgccg cagcagcaag ggtgcccgcc gcgagcgcgc ccatcatcca gcgtcgagca
     1081 ccgcgcggtc gctcgagccg cgccgagacg ttaccccaga gccgcgcgat tcgcgcctcg
     1141 gagacgtcca cgacgacgtg gcgcgaccta tcgctcgccg gttcggtcaa gcaattcctc
     1201 cgcggccttc aagcgccgct tcacggtcga caacgacaag cccatggttt cggcaatctg
     1261 cgggagctcg agcccgtcga cacgacgtag cacgagcgcg atgcgctgct cagcgggcag
     1321 ctcttccacg atggcgtaga cgcgccgcag cgcgacgcgg acgtcgcccg gcgccgaagc
     1381 ggcgatcact tgatccggat caacactttc acgtgcgcgc agaccgaggc gcgtcagcag
     1441 tcggcgacgc cgcaagcgct tgctcacagt acggaccacg atggacccga gccatgccgc
     1501 gaaggcttgc gggttctgca acgagtccag tcgctgcagc gcgtaaacga agctatcttg
     1561 cacgacgtcg tccacttcga cgtctcgcgg caagagccgg taggcgagcc cgagcgccat
     1621 gcgtgaatgt cgacggaaca gcgcttcact cgcccacacc tcaccggctc gtgcggccac
     1681 gaccagcgcc gcatcgctcg gcccggcacc agacttggtc ggcggaatcg ccgccgcagg
     1741 cgtcatcggc cgcggcatac tccccccgcg cgcactggca gcggccggag cggcgccagc
     1801 gagcggcgtc gtatccgagg ttcgcgcggg aagaactgca tgcatgattc tgcgccggac
     1861 tcgttgccgc gcgcagagta cgtacccgga gcccccgcca acggttcagc ggcaccccct
     1921 gacagcccag cggccgccgg gcctattcgc cgcgaaccag gccgacgccg cgctttggcg
     1981 cgttttttcg ggcacttaaa ggttggggac gaacggcttc ttgttcacga cagcgacgtc
     2041 ccaccagcga aacgccatgg cgatggccac gtcgagctcc cgcgtttctt tgcaagggtc
     2101 gagcgagaga gggggcatgc agtatgccgc gatcgggcac gccgcgagca tgtgcagaat
     2161 gccgtccgaa aagccgccgc ctgcgtaggc gaggcaaacg ggcattccca tcgcctgcat
     2221 gtcctggatc gcgtaaggga tcttctcgtc gacggtgccg acgctgtctt gatacttaca
     2281 ttcgatcgca aaagcggtgc ccgacgcggc
//
LOCUS       DASTKS010000440         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_70007,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000440 DASTKS010000000
VERSION     DASTKS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70007"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFQ35_09215"
     CDS             complement(<1..929)
                     /locus_tag="VFQ35_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9930854.1"
                     /translation="MVTPRPFRSARRAPKARGFTLVELLVALTGGLFVSIAVFAMARD
                     ATRFYQREGRLASATLAAVVGFERLRADVARAGFLSSPNIQADPLVCNRPAAGAPSRL
                     LNLASAWVGTGATGSPSNSVLDTYLASSPGKRAHALVLSGSYSSPDEFPVRLVMPLGN
                     DGSDGYGVYLQLGTGALARLGWASATTDAARTLLLSQVFAQGRVLRVVDKEGRSQFGV
                     ISSVSVDAANREPFIRLSATPALVFRYGQSRLCGFKGNETGSTANVVNFIRYDVRNLK
                     SASLFSGTTKYASLYASSTNAALDPYEKNRTELV"
     gene            complement(923..1555)
                     /locus_tag="VFQ35_09220"
     CDS             complement(923..1555)
                     /locus_tag="VFQ35_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9930855.1"
                     /translation="MTTRVGRSRRRLSRGYTFVELLMAMMIFSIGVTGVIAMQKVTLT
                     SNQHARDLAMATQIAQSWMDQLHADAMAWNHPSALRATNDLSDTVWLSTVAQNNATWT
                     RPDWNATRRFGSAFDARGTSINQVDSPNQVKYCTNIRLSWLYQPVGVVTGGGLMRAEV
                     RVFWLRDGQPRAGNVAFCSNQMDVSAIGADVARYHFVYQVSAVRENTAQW"
     gene            complement(1552..2172)
                     /locus_tag="VFQ35_09225"
     CDS             complement(1552..2172)
                     /locus_tag="VFQ35_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9930856.1"
                     /translation="MNVPAAAGHFLRSKRLRASRGFTLVELMVVVLIVAILAVIAVPS
                     VVERMRERRSSEAAQRIAGLYRGARMRAMGRGSAVMVSYDQGSFIVREAVRPPPADNP
                     QCTGEPWSSCTNNDWSNTKNYSELSRFAPTKRSEYEGLTLAAIPSTTTRIDICFTPMG
                     RAYTRADNLPFSTPMVQVASFNVGRSGSAITRTVMVLPNGVARVAL"
BASE COUNT          396 a          703 c          770 g          360 t
ORIGIN      
        1 acgagctcgg tgcggttctt ctcgtacgga tcgagggccg cgttcgtgct cgacgcgtac
       61 aggctcgcgt acttcgtggt cccgctgaag agcgacgcgc tcttcaaatt gcggacgtcg
      121 tagcgaatga agttcacgac gttggcggtg gacccagtct cgttgccctt gaagccgcag
      181 agccggctct ggccgtagcg aaagaccagc gccggagtgg cgctcaggcg gatgaatggc
      241 tcgcggttcg ccgcgtcgac ggaaaccgac gaaatgacgc cgaactgcga gcgaccctcc
      301 ttgtcgacga cgcgcagcac gcggccctgg gcgaagacct gcgatagcaa cagggtgcga
      361 gccgcgtcgg tcgttgcgct cgcccaaccg agccgcgcaa gcgctccggt gccgagctgg
      421 aggtacacgc cgtaaccgtc ggacccgtca ttgccgagcg gcatgaccag gcgcaccgga
      481 aattcgtcgg gtgagctgta ggagccgctg agcacgagcg cgtgggctcg cttgccaggg
      541 gagctcgcga gataggtgtc gagcaccgaa ttgctgggcg agccggtcgc gccggtgccg
      601 acccaggcgc tcgcgaggtt cagcaagcga gaaggggcgc cggccgccgg acggttgcag
      661 accaggggat ccgcctggat gttcggggag ctcaggaacc cagcgcgcgc gacgtcggcg
      721 cgcagccgct cgaagccgac caccgcggcg agcgttgcgc tcgcgagccg cccttcgcgt
      781 tggtagaagc gtgtcgcgtc gcgcgccatc gcaaagaccg caatcgagac gaagagaccg
      841 ccggtgagcg cgaccaggag ctcgaccaag gtgaagcctc gggccttggg cgctcgacgc
      901 gcagagcgaa acgggcgagg ggtcaccatt gagccgtgtt ctcccgaacg gccgagacct
      961 ggtagacgaa atggtaacgc gcgacgtccg cgccgatcgc cgagacgtcc atttgattgc
     1021 tgcagaacgc gacgttgccg gcgcgcggtt gcccatcgcg cagccagaag acgcgaacct
     1081 cggcgcgcat gagcccgcca ccggtgacca ccccgacggg ctgatacagc cacgacaggc
     1141 ggatgttcgt gcagtatttc acctggttcg gtgaatcgac ctgatttatc gacgtgccgc
     1201 gcgcatcgaa ggccgacccg aagcgtcgcg tcgcgttcca atccgggcgg gtccaggtcg
     1261 cgttgttctg agctacggtc gacagccaga cggtgtcgct gagatcgttc gtggcgcgaa
     1321 gggccgaagg gtggttccac gccatcgcgt cggcgtgcag ctgatccatc cagctttgcg
     1381 cgatttgggt cgccatcgcg aggtctcgcg cgtgctggtt cgacgtcagc gtcaccttct
     1441 gcatcgcgat gacgccggtg acgccgatcg aaaagatcat catcgccatc aagagctcga
     1501 cgaaggtgta gccgcgcgag agtcgacggc gggatcggcc gacgcgagtg gtcacaacgc
     1561 gaccctcgcc acgccgttcg ggagcaccat caccgtgcgc gtgatggcgc tgccgcttct
     1621 tccgacgttg aagcttgcga cctgaaccat cggcgtcgaa aacggtaggt tatcggcgcg
     1681 cgtgtacgct cggcccatcg gggtgaagca gatgtcgatt cgcgtcgtcg tggacggtat
     1741 cgccgcgagc gtgaggccct cgtactcgct gcgcttcgtc ggggcgaagc ggctgagctc
     1801 gctgtagttc ttggtgttgc tccagtcgtt gttggtgcag ctgctccacg gctcgccggt
     1861 gcactgcggg ttatctgcag ggggaggccg aaccgcttcg cgaacgatga aacttccctg
     1921 gtcgtagctg accatcaccg ccgaaccgcg gcccatcgca cgcatccggg cgccgcggta
     1981 aagtcctgcg attcgctgcg ctgcctcgga agaacggcgc tcgcgcatcc gctccaccac
     2041 cgatggcacc gcgatcaccg ccagaatcgc gacgatgagc acgacgacca tcagctcgac
     2101 gagcgtgaag ccgcgcgagg cgcgaaggcg cttcgagcga aggaagtgac cggcggcagc
     2161 agggacgttc acgggggcga atcctcgaga gcaattggcg tgccgtcgat gcgtagcaga
     2221 tactacgga
//
LOCUS       DASTKS010000441         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1855105, whole genome shotgun sequence.
ACCESSION   DASTKS010000441 DASTKS010000000
VERSION     DASTKS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855105"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFQ35_09230"
     CDS             complement(<1..351)
                     /locus_tag="VFQ35_09230"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HET9930857.1"
                     /translation="MLPREKLKSLVRRHQEVESLLCDPGVLADMKKLNSLNRERGQLV
                     PIVEAMGKLEGVERRIREDEEALDDPDLGPLARQELPELQAEQKGLEQELRLLLLPKD
                     PNDEKNTILEIRGGT"
     gene            complement(353..1489)
                     /locus_tag="VFQ35_09235"
     CDS             complement(353..1489)
                     /locus_tag="VFQ35_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9930858.1"
                     /translation="MSNQPAVVGPDGVARPYIGGQAVLEGVMMRSPHAFAVVVRRRNG
                     ELVVMEEPMTDLRKGARAWPLVRGVTALVEALKLGSRALRFSADVYERDFNEAEANAR
                     ASKPSLLSLLSLPIIALVTSEPELPSASSSKSKGGGMMSVLSVLFALGIFIALPQAFA
                     EIFGNRILKLDLDVRSPGFQALTGAAKLVVIVGYMLAIRRIPDIYRVFQYHGAEHKSI
                     HTYEAGEELTVENARKRTTLHPRCGTTFIVMVALVSIFVFSLLGPFLPSFGLNKLADN
                     VLFFLFKLPFLPLIAAVTFELQRLLARFGKTGPLRALLWPGFLVQKITTIEPDDAQLE
                     AAMGSLALALAHEQGVPRTVAAEKSYPNYAELLSRGTGPQLSAG"
     gene            complement(1662..1874)
                     /gene="rpmE"
                     /locus_tag="VFQ35_09240"
     CDS             complement(1662..1874)
                     /gene="rpmE"
                     /locus_tag="VFQ35_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9930859.1"
                     /translation="MKDGIHPEYEVVSVSCACGNTFQTRSTKKELTADVCGACHPFYT
                     GKQRLVDTQGRVDRFRRKYAASGAKK"
     gene            complement(2173..2586)
                     /locus_tag="VFQ35_09245"
     CDS             complement(2173..2586)
                     /locus_tag="VFQ35_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9930860.1"
                     /translation="MSQRRTHLRGASSRPVGDARSAYLAAWRWRREVELALRPLDLTF
                     TQWLVLDATASAISELEDDHAVSQREVAEFCELDPMTVSQVMTTLALRGLVDRGPSAT
                     GRSYRIILSASGRRTLQSAASHIKAATERFWAGER"
     gene            3347..4291
                     /locus_tag="VFQ35_09250"
     CDS             3347..4291
                     /locus_tag="VFQ35_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET9930861.1"
                     /translation="MLERIGAAGAVLGGAALASRLLYDHGGLELTRPTGERQVRDFKS
                     NVPGPAPVYAIAKSSTEPEELVRRAVAAMGGMKRFVSRGDIVAVKPNIGWDRMPVHAA
                     NTNPRVVAEVVRLAFDAGAKQVVVTDASCNEPNRCFQRSGIWRATYALGATVILPSAH
                     RFREIRMRGDVLDQWPIYTPLVNADKVINVPVAKHHNLSKYTAAMKNWYGILGGRRNR
                     LHQNIDVSIADLATFMRPTLTIVDATRVLLRNGPQGGNIDDARDMHQVIASLDQVAVD
                     AYGATLIGEKPERVRYLKLGHERGIGNMDFMNVPHVEV"
     gene            4292..>4607
                     /locus_tag="VFQ35_09255"
     CDS             4292..>4607
                     /locus_tag="VFQ35_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930862.1"
                     /translation="MAAPQRHGHGTRVDALGAQREDAGHAHVGQAERGHVGDAGHARN
                     APAGHSGSALVGGAADAAEFPSPLPWLTNAKPGPVEALDAPSKSGPRNALRAEASATG
                     ARG"
BASE COUNT          866 a         1408 c         1535 g          798 t
ORIGIN      
        1 ggtgccgcct cgaatttcga ggattgtatt cttctcgtcg ttcggatctt tgggcagcaa
       61 caagaggcga agctcttgct cgagcccctt ttgctcggct tgcagctccg gcagctcctg
      121 ccgcgcgagc ggccccaaat caggatcgtc gagcgcctct tcgtcttcgc ggatacgccg
      181 ctcgacgccc tcgagcttgc ccatcgcctc gacgatgggg acgagctgtc cgcgttcgcg
      241 gttcagcgag ttcaacttct tcatgtcggc gagcacaccc gggtcgcaca gcaagctctc
      301 gacctcttgg tgtcgcctga ccaagctctt cagtttttcg cgcggaagca tctcagcctg
      361 cggacaattg cggacccgta cctcgggaca agagctccgc atagttcggg taactcttct
      421 cggccgcgac ggtgcggggg acaccttgct cgtgcgcgag cgcgagcgca agcgacccca
      481 tcgcggcttc gagctgagcg tcgtctggtt cgatggtcgt gatcttctgc accaagaagc
      541 ctggccacaa gagcgcgcgc agcgggccgg tcttgccgaa gcgggcgagc aagcgctgga
      601 gctcgaaagt gacggccgcg atgagcggca agaacggtag cttgaacaag aagaacagaa
      661 cgttgtcggc gagcttgttc aggccgaacg agggcaagaa cggtccgagc aacgaaaata
      721 cgaagatcga cacgagcgcg accatcacga tgaaagtcgt gccgcagcgg gggtgcaacg
      781 tcgttcgctt gcgcgcgttt tcgaccgtca gctcctcgcc cgcttcgtag gtgtgaatcg
      841 atttgtgctc ggcaccgtga tactgaaaaa cgcgataaat atcggggatg cgccgaatgg
      901 ccagcatgta gccgacgatg acgacgagct tggcggcgcc ggtaagggcc tggaagccgg
      961 ggcttcgcac gtcgaggtcg agcttcaaaa tgcggttccc gaagatctcg gcgaacgcct
     1021 gcggcaacgc gatgaagatg ccgagcgcga acagcacgct caacaccgac atcataccgc
     1081 cgcctttgct cttcgaactc gacgcagacg ggagctccgg ctcgctcgtg acgagggcga
     1141 tgatgggcag cgagagcagg ctcaagagcg agggcttgct cgctcgcgca ttcgcctccg
     1201 cctcgttgaa gtcgcgctca tagacgtcgg ccgaaaagcg aagcgcgcgg ctgccgagct
     1261 tcagcgcctc gacgagggcc gtgaccccgc gcaccagcgg ccaggcgcgc gcgcctttgc
     1321 gcaaatcggt catcggctct tccatcacga cgagctcacc gttgcggcga cgcaccacga
     1381 cagcgaacgc atgcggcgag cgcatcatca cgccttcgag aacggcctga ccgccaatgt
     1441 acggacgcgc tacgccgtcg ggaccgacca ccgcaggttg attcgacatc ttctcgcagt
     1501 atagcccgag cgccgtcgcg aagctttacg cgacgtatgt ttcgaaccag cgcgacatcg
     1561 aaccagcgac accgaacatg cgccgcacga ctgcgctcga gcgcgcgcaa tgaggcgcac
     1621 tcgagcgcga aacggcgaag ctcggtttta ttcccgagcg cttacttctt ggcgccgctc
     1681 gccgcgtact tgcggcggaa acgatcgacg cgaccctgcg tgtcgaccaa gcgctgcttg
     1741 cccgtgtaga acggatgaca agcgccgcac acgtccgccg tcagctcttt cttcgtcgag
     1801 cgcgtttgga acgtgttgcc gcacgcgcaa ctaaccgaca caacctcgta ttcggggtga
     1861 atcccgtcct tcatgacatt ctccaaatca gcgcgtgagc tcggcccgcc tgctgggccg
     1921 aaaaagggac gcggacgcta gcgccgagcg ccacaacgtt caaggccgtt cgcacgattc
     1981 gcaaactgct cggccctcta ctgtacgttt aaccagcctt cagccagagt cccgactgct
     2041 tcgcagaagg atctaagccc gacccgcgaa agcgcggcga acgagccggc cctctacctt
     2101 ggggatgagg gggcactctg taaagggctc ataaagggct ccgggcgccc gaggaccgaa
     2161 cggtcgctgc tattaccgtt cccctgccca aaaccgctcg gtggccgcct tgatatgact
     2221 cgcagcggac tgaagcgtcc ggcgtccact ggcgctcaaa atgattcggt aggaccggcc
     2281 cgttgccgaa gggccgcgat ctaccagccc tcgcagcgcg agcgtggtca tcacctgaga
     2341 tacggtcatc ggatcgagct cgcagaactc cgcgacctct cgctggctga cggcgtggtc
     2401 gtcctcgagc tccgaaatgg cggaggcggt cgcatcgagc acgagccatt gcgtgaatgt
     2461 caggtccaaa gggcgcagcg cgagttccac ctctctgcgc cagcgccaag cagcgagata
     2521 ggccgagcgg gcgtcgccaa ctgggcgcga agacgctccg cgcaagtgag ttcgtcgttg
     2581 cgacatcgtc tatgtataca tatcaaagcg cgtcacagga aaccgcataa atcctgcggc
     2641 cttgaagacg cgccgacccc gatacggcac ccattcgcac aagcggcatc ccacggcgga
     2701 cgcaacttat tgaaattact gcattcgtct gccgggccaa aatgcatgta tgcatactcg
     2761 ggtctcagga ttcatcgcat ctccgaacca cgcgacggcc cgcagttttt cggggttccc
     2821 gggcccccga aagccacaca aaccaaagca tcggaacgaa ctctttcgaa gcacgggtcg
     2881 gaaccaactc tttcgtgccg agccctctgc gagcgaacga gacgaaggcc tacctgctgc
     2941 ggcagatggc tctggcgcgg cagatggctc tggcgcgagg gagttgctgg aggtcgcttt
     3001 cgcgcgggag tagctccggc gctggggatt tctgcggccc ggaggattgc tggaagtcgc
     3061 tccgacgcgg agtcacatcg ctctggcgcg ggggattgct ccggtgggat tggttggcgg
     3121 agcggtcggc ggagcggtcg gcggagcggg ggacgtgggc ggtcggcgga ttggtttgcg
     3181 ggaagctggg tctggtgaaa tcgcccgctc acgccggaat ccggagcgaa gtcataatga
     3241 taacgagaga gtagagcggt gctagactcc cgaccgcaaa cttgacgtta cgtcaaccct
     3301 cgcccaaggt gcctgcacga tgacggaccg gccgacgcgc cgagagatgc tggagaggat
     3361 cggcgctgcg ggagcggtgc tgggcggagc cgcgctggct tcgcgcctgc tctatgacca
     3421 cggtgggctg gagttgacgc ggccgacggg tgagcgtcag gtgcgcgact tcaagtcgaa
     3481 tgtgccgggg ccggcgccgg tctacgcgat cgcgaagagc agcacggagc cggaagagct
     3541 cgtgcggcgc gccgtggccg cgatgggcgg gatgaagcgg ttcgtttcgc gcggcgatat
     3601 cgtcgcggtg aagccgaaca tcgggtggga tcggatgccc gtgcatgccg cgaatacgaa
     3661 tcctcgcgtc gtcgcggagg tggtgcgact cgcgttcgat gcgggcgcga agcaagtggt
     3721 cgtgacggat gcgtcgtgca acgagccgaa tcgatgtttt cagcggtcgg ggatttggcg
     3781 cgcgacgtac gcgctgggcg cgacggtgat tttgccttcg gcgcaccggt tccgcgagat
     3841 tcgcatgcgc ggcgatgtgc tcgaccagtg gcccatctac acgccgctcg tgaacgccga
     3901 caaggtgatc aacgtgccgg tggcgaagca ccacaatttg tcgaagtaca cggcggcgat
     3961 gaagaattgg tacggcatcc tcggtgggcg ccgtaatcgg ttgcaccaga acatcgatgt
     4021 ttcgatcgcg gatcttgcga cgttcatgcg tccgacgttg acgatcgtgg atgcgacgcg
     4081 cgtgctgctg cgtaatggtc cgcagggcgg gaacatcgac gacgcgcgcg atatgcatca
     4141 ggtgattgcg agcctcgatc aggtcgcggt ggacgcgtac ggcgcgacgt tgatcggcga
     4201 gaagccagag cgtgtccgct atttgaagct gggacacgag cgcggcatcg ggaacatgga
     4261 tttcatgaac gttccgcacg tggaggtcta agtggcagcg cctcaacgcc acggtcacgg
     4321 aacccgcgtc gacgctctcg gcgcgcagcg ggaggacgcg ggtcacgccc acgtgggtca
     4381 agccgagcgc ggccacgtgg gtgacgcggg tcacgcgcgc aatgcgcctg caggtcactc
     4441 ggggtccgct ctcgttggtg gcgcggcgga cgcggcggag tttccgtcgc cgttgccgtg
     4501 gctcacgaac gcgaagccgg ggccggtcga ggctttggat gcaccgtcga agagcgggcc
     4561 gcggaatgcg ttgcgcgcgg aggcgtcagc gacgggagcg cgcgggg
//
LOCUS       DASTKS010000442         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1184868, whole genome shotgun sequence.
ACCESSION   DASTKS010000442 DASTKS010000000
VERSION     DASTKS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184868"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFQ35_09260"
     CDS             <1..879
                     /locus_tag="VFQ35_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930863.1"
                     /translation="RDALPEANQVSVAGPEHDSSGKKTLAIGGGGAEVAEWYAFTRNV
                     RDGVNVVTRDVLAGVWLVVHSPPSKVGMDFAEWGPYTDALDPAAYRMRVERSPDGTYL
                     YRLEGRPRSSSSEADYVAVLTGKGYGKLDSRHGDGEFTIDLDAAKRLDPIKHRDDSGT
                     VSITHDLPAEDPSALPRIIAAQVDPAGDAWVRIRSEAKVDLTGTLSVDAHADTDESKA
                     TAMEDISLVSRWKADGQGRADVSIAGGDLPAAIPLVTASECWNDSFARVYYSDSVGFK
                     PVFGDSTACVYSDPAL"
     gene            938..2572
                     /gene="pruA"
                     /locus_tag="VFQ35_09265"
     CDS             938..2572
                     /gene="pruA"
                     /locus_tag="VFQ35_09265"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET9930864.1"
                     /translation="MTEPVNEPVLTYAPASPERASLRAEIARQKSTLTCLRSRIGDRQ
                     LASSTVESVRAPHRHELEIARAEFVGRSEVQSAIDAALAAQSSWAALDLPSRAAIFRR
                     AAELAAGPFRQRLNAATLLGQSKTPHQAEIDSACELIDFLRFNAYFAERLAEEKLIST
                     PFEQNRFDFRPLEGFVYAVTPFNFTAIAANLPTAPALLGNVVVWKPSPYALLSAHYVF
                     ELLREAGLPPGVLNLVIGDAREVSDEVLSHTDFAGLNFTGSSAVFEQLFQRVASRLGH
                     YKNYPRLVGETGGKDFIVAHSSADLDALAVAILRGGFEYQGQKCSAASRVYVPRSLAA
                     ALEARLLELVGTIRVGDPENFENFMGAVIGRAAYDRITGAIERAKSDPQCRIVFGGEA
                     DDRDGFFINPTLIEVKASPHFLLSEELFGPVVAMLVYDDDRYDEVLRACDADSRYALT
                     GSIFAEDQAAIDLASRTLRFAAGNFYINDKPTGAVVGQQPFGGARRSGTNDKAGCIFH
                     LLRWISPRVTKHNLRPPKNYRYPFLEPDDPNPKGTP"
     gene            2569..>3316
                     /gene="hemH"
                     /locus_tag="VFQ35_09270"
     CDS             2569..>3316
                     /gene="hemH"
                     /locus_tag="VFQ35_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET9930865.1"
                     /translation="MSDGILLTAHGTVENLDEMPEFLRRIRHGRPAPPELVEELKRRY
                     TTIGGSPLLRITNEQADLLARETGLPVFIGMRLFRPELSDALQKAHAAGITRLVVLPL
                     APFSVHVYAAAAERVCKELNLPLTLAVGRPYGDAEEFVQAQADLVRPALGAPESESLL
                     LTAHSLPRKVIEMGDPYAKLVELSASAIAKKLGRAHELVYQSQGADGGDWLGPDLRSA
                     LSAAQARGVRRVVVAPVGFLSDHVETLYDLD"
BASE COUNT          577 a         1122 c         1064 g          553 t
ORIGIN      
        1 cgcgacgcgc tgcccgaagc gaatcaagtg agcgtcgcgg ggcccgaaca cgactcgtcg
       61 gggaagaaga cgctcgcgat cgggggaggg ggagcggagg ttgcagagtg gtacgccttc
      121 acgcgcaacg tgcgcgacgg cgtgaacgtc gtcacacgcg acgtgctcgc gggggtctgg
      181 ctcgtcgttc attcgccgcc gagcaaggtc ggcatggact tcgccgaatg gggcccttac
      241 accgatgcgc tcgaccccgc cgcctaccga atgcgcgtgg agcggagccc ggacggaacg
      301 tacctgtacc gtctcgaagg gcggccgcgg agctcgagct ccgaagcaga ctacgtcgcc
      361 gtgttgaccg gcaaaggtta cggaaagctc gattcgcgtc acggcgacgg tgagttcacc
      421 atcgacctcg acgcggccaa gcgcctcgac ccaatcaagc accgggacga ttcgggtacg
      481 gtgagcatca cccacgactt gcccgccgag gatccctcgg ccctgccgcg gatcatcgct
      541 gcccaggtgg accctgcggg ggatgcgtgg gtgcggatcc gatccgaggc gaaggtcgac
      601 ctcaccggga ctttgagcgt cgatgcgcac gcggataccg acgagagcaa agcaaccgcg
      661 atggaggaca tttcgctcgt cagtcgctgg aaggccgacg gtcaaggtcg cgcggatgtg
      721 tcgatcgcgg gcggggattt gccggccgca attcccctcg tgaccgcgag cgagtgctgg
      781 aacgacagtt ttgcgcgcgt ctactactct gactcggtcg ggttcaagcc cgtcttcggc
      841 gactcgacgg cctgcgtgta cagcgacccg gcgctctgag cagagccgcc tgaaatcgct
      901 cgccgggaac gcgtagcgga actacgcttc gcgagtggtg accgagccag tcaacgagcc
      961 ggttctgacc tacgccccgg cgagccccga gcgtgcctcg ctccgcgcag aaatcgcgcg
     1021 ccagaagagc acgttgacgt gcttgcgctc ccgcatcggg gatcgtcagc tcgcctcgag
     1081 cacggtcgag agcgtacgcg cgccgcaccg ccacgagctc gagatcgccc gcgccgaatt
     1141 cgtggggcgg agcgaggtgc agtcggcgat cgacgcggcg ctcgcggcgc agtcgagctg
     1201 ggcagcgctc gacctcccga gccgcgccgc gatctttcgg cgcgcggcgg agctcgcggc
     1261 aggtccgttc cgccagcggt tgaacgcggc gacgctgctc gggcagagca agacgccgca
     1321 ccaggcggaa atcgactcgg cttgcgagct catcgatttt ctccggttca atgcatactt
     1381 cgccgagcgc ctcgccgaag aaaagttgat ctcgacgccg ttcgagcaaa accgcttcga
     1441 ctttcgcccg ctcgaaggct tcgtctacgc ggtcacgccc ttcaacttca ctgcgatcgc
     1501 ggcgaatctt cccaccgctc ccgccttgct cggcaacgtg gtcgtttgga agccgtcacc
     1561 ctacgctctg ctcagcgccc actacgtttt cgagttgctc cgcgaagccg ggttgccgcc
     1621 cggggtgctc aacctcgtca tcggcgacgc ccgcgaggtg agcgacgagg tcctctcgca
     1681 caccgacttc gcggggctca atttcacggg gtcgagcgcg gttttcgagc agctcttcca
     1741 gcgcgtcgcg agccgccttg gtcactacaa gaactacccg cgcctagttg gtgaaaccgg
     1801 gggcaaagac ttcatcgtgg cgcactcgag cgccgatctc gacgcgctcg cggtcgccat
     1861 cctacgcggc ggcttcgagt atcagggcca aaagtgctca gccgcctcgc gcgtgtacgt
     1921 tccgcgctcg ctcgcggcgg ctctggaggc gcgcctgctc gagttggtcg gcacgatccg
     1981 cgttggcgac ccggaaaact tcgaaaactt catgggtgcc gtgattggtc gggcggcgta
     2041 cgaccgcatc accggcgcga tcgagcgggc aaagagcgac ccgcagtgcc gcatcgtgtt
     2101 cggaggcgaa gccgacgatc gagacggctt cttcatcaat cccacgctca tcgaagtgaa
     2161 ggcgtcgccg cacttcttgt tgagcgagga gctcttcggg ccagtcgtcg cgatgctcgt
     2221 ttacgacgac gaccgctacg acgaggtgct gcgcgcctgc gacgccgaca gccgctacgc
     2281 gctcaccggc tcgatcttcg cggaggacca ggcggcaatc gacctagcga gccgcacgct
     2341 gcgctttgcc gctgggaact tctacatcaa cgacaagccc accggcgcgg tcgtgggcca
     2401 gcaaccgttc gggggcgctc gccgctccgg cacgaacgac aaggctgggt gcatcttcca
     2461 cttgctgcgc tggatctccc cgcgcgtgac gaagcacaac ctcaggcccc ccaagaacta
     2521 ccgttaccct ttcttggagc cggacgaccc aaatcctaaa ggcacacctt gagcgacggc
     2581 attctcttaa cagcgcacgg caccgtcgaa aatctcgacg aaatgccgga atttttgcgg
     2641 cgtattcgcc acggacggcc ggcaccgccc gaactcgtcg aggagctcaa acgccgctac
     2701 accaccatcg gcggctcccc actgctccgc atcacgaacg aacaggccga tttactcgct
     2761 cgggagaccg gtttgcccgt cttcatcggc atgcggctgt ttcgcccgga gctcagcgac
     2821 gcgctccaaa aggcgcacgc agccggaatc acgcggctcg tggtgctacc gctcgcgccg
     2881 ttcagtgtcc acgtctacgc ggcggcggcg gagcgcgttt gcaaagagct caacctcccg
     2941 ctcacgctcg ccgtcggacg accgtatggc gacgcggaag agttcgtgca ggcccaagcg
     3001 gatttggttc gccctgcgct cggcgctccc gaatccgaat cgctgctgct caccgctcac
     3061 agcctgcccc gaaaagtcat cgagatgggc gatccctatg caaagctcgt cgaactcagc
     3121 gcgagcgcca ttgcgaaaaa actcgggcgc gcccacgaac tcgtgtacca gagtcagggc
     3181 gccgacgggg gtgactggct cggccctgat cttcggagcg cgctttcggc cgctcaggcg
     3241 cgcggcgttc ggcgcgtggt cgtggcgccg gtggggttcc tcagcgatca cgtggagacc
     3301 ctctacgatc tcgacg
//
LOCUS       DASTKS010000443         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1855210, whole genome shotgun sequence.
ACCESSION   DASTKS010000443 DASTKS010000000
VERSION     DASTKS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855210"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2149
                     /locus_tag="VFQ35_09275"
     CDS             18..>2149
                     /locus_tag="VFQ35_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9930866.1"
                     /translation="MATDAIEIGAGEGWCEPHVAPDDVALLQYTSGSTAEPRGVMVSH
                     RNALHNSAAIHRLAQHDERSVYVCWLPSYHDMGLIGGILQPIYGGLLGVLMSPASFLQ
                     SPVRWLRALSRFGGTTTPFPNFALDHCVRRISAAERDEVDLSALRVACNGAEPVRWET
                     LQRFARTFSPRGFHPEAHYPAYGLAEATLLVSGRSPSDSLQALRVDRAALAAGRVEGG
                     EVLTLNGTDEASTGAAKWAALVSCGRSLPDQELLIVEPEARCPVGPDCVGEIWVRGPS
                     IAQGYFRRPAETAETFGARLADGSGPFLRTGDLGFLRDGELYVTGRRKDLIILRGRNM
                     YPQDIERTAERCHRQLRPGGVAAFAIEGEGEERLALAAEVDARSGPPDWDGIWVALQE
                     AVAREHDVALSVATLLSSGTLPKTSSGKVQRRACREALVRGELTVLRRFSVSVPAAVT
                     PTSESAIAHTGADCTSLYDALHARVARLDRRSTRYRYDLERDVPWHRLDEAGLYAGPS
                     LLASERIDLSSVANTQAADLLQWTLAVNICDRFIALEEAVLRFTAEERAALGGTRSIE
                     LLDAEEEKHIAMFRRYGEHLRRLRPELAPDADRYLAAARERLAETFRRLTTPLERHYA
                     NWVLIVFFEEYSLFMYRELLRDAPALQPVWLAIHRAHAREEAQHVQTDAAYLAALRVS
                     EEQRYAWSRGIFDALAADFSVLFALLGE"
BASE COUNT          321 a          693 c          730 g          405 t
ORIGIN      
        1 tcgccagccg tcgttgggtg gcgactgacg cgatagagat cggagcaggc gaggggtggt
       61 gcgagccgca cgtagctcca gacgatgtcg cgctcctgca atacacgtcg gggtccacgg
      121 ccgagcctcg tggcgtgatg gtgagtcacc ggaacgcgct gcacaattct gctgcgattc
      181 accgcctcgc tcagcacgac gaacgcagcg tgtacgtctg ttggctgccg agctatcacg
      241 acatgggtct catcggcggc attttgcagc caatctatgg aggattgctg ggtgtgctga
      301 tgtcaccggc ttcgtttctg cagagcccgg tgcgctggct gcgagcgttg tctcgttttg
      361 gcggtacgac gacgccgttc ccgaactttg cgttggacca ttgcgtgcgc cgaatctcgg
      421 ctgccgagcg cgacgaggtc gatttgtcgg cgctgcgcgt tgcgtgcaac ggcgcggagc
      481 cggtgcggtg ggagacgttg cagcgctttg cgcggacgtt ctcgccgcgc gggtttcacc
      541 ccgaggcgca ctatcccgcc tacggtctcg cggaagctac tctgctcgtc agtggccgct
      601 cgccgagcga ctcgctccag gcgctccgcg tcgaccgagc cgcattggcg gccggtcgcg
      661 tcgaaggcgg cgaggtactc actctcaatg gaaccgacga agcgtcgact ggtgctgcca
      721 aatgggcggc gctcgtaagc tgtgggcgca gtttgcccga ccaggagctg ctcatcgtcg
      781 agcccgaggc gcggtgtccc gttggtcctg attgcgtcgg agaaatttgg gttcgtgggc
      841 ccagcatcgc gcagggttac ttccgccgac cggcagagac cgccgagact tttggcgcac
      901 gtctcgccga tggctcgggt ccatttctgc gaacgggcga cctcggcttt ttgcgcgatg
      961 gcgagctgta cgtcaccggt cgccgcaaag atctcatcat tttgcgcggc cggaacatgt
     1021 acccgcagga tatcgagcgc acggcggagc gctgtcatcg ccagttgcgc cccgggggcg
     1081 tcgccgcctt cgccatcgag ggcgaaggcg aggagcgctt ggcgctcgcc gcggaagtcg
     1141 atgctcggag cgggccgccc gattgggacg gcatctgggt tgcgcttcag gaggcggtag
     1201 cgcgagagca cgacgttgct ctttcggtcg cgacgttgct ctcctcgggg acgctgccaa
     1261 agacatcgag cggcaaggtg cagcgcaggg cctgtcgcga ggcgttggtg cgcggtgagc
     1321 tcacggtgtt acggcgcttt tccgtgtcgg ttccggcggc cgtgactccg acgagcgagt
     1381 cggccatcgc ccacaccggg gctgactgca cttcgcttta cgatgcgctg cacgctcggg
     1441 tggcacggct cgaccgccgc agcacccgtt accggtacga cctcgagcgc gacgttccgt
     1501 ggcaccgcct cgacgaggcc gggctctacg ccgggccgag tctgctcgcc tccgagcgca
     1561 tcgatctctc gagcgtcgcg aacacgcaag cggcagacct tctgcaatgg acgctggcag
     1621 taaatatctg cgaccgcttc atcgccctcg aagaggcggt gctgcgcttc acggccgaag
     1681 agcgggcggc gctcggcgga acgcgaagca tcgagctcct cgacgccgaa gaggagaagc
     1741 acatcgcgat gtttcgccgc tacggcgagc acttgcgacg gcttcgcccc gagctcgctc
     1801 ccgatgcgga tcggtacctt gccgcagctc gggagcggct ggcggagacg ttccgccgct
     1861 tgaccacccc cctcgagcgg cactacgcga attgggtcct catcgtattc ttcgaggaat
     1921 actccctctt catgtaccgc gagctcctgc gcgacgcgcc ggcgctgcaa ccggtgtggc
     1981 tcgcgatcca tcgcgctcac gcccgcgaag aggcgcagca cgtgcagacg gacgcggctt
     2041 atctcgcggc tcttcgcgtg tccgaggagc aacgctacgc ttggtctcga ggcatcttcg
     2101 atgccctcgc agccgacttc tcggtgctgt tcgcgctgct cggtgagca
//
LOCUS       DASTKS010000444         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_738413, whole genome shotgun sequence.
ACCESSION   DASTKS010000444 DASTKS010000000
VERSION     DASTKS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738413"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFQ35_09280"
     CDS             <1..204
                     /locus_tag="VFQ35_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930867.1"
                     /translation="ADGRSFGSCRERRVPSECAGAAGRDGDIAKLEQCCIAHGYCCVD
                     SEDLDHDGDRAEVLGNCEDVSCD"
     gene            339..1181
                     /locus_tag="VFQ35_09285"
     CDS             339..1181
                     /locus_tag="VFQ35_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930868.1"
                     /translation="MAASLAALVTLFAQATYADDCGRPNLVATFPIDGASAVPLNAKL
                     SASYAESADYLGEAVTLASETGDRTLDARFDASERRLIVDEPALEPNTHYTLAWPALR
                     GISTAGHGSTKTISFTTGDALDAAAPDFQGITKLAWDLVHPRDECTDDLEPRMRFSFG
                     LGEARDDGGAESLELLLFQTSPGSADAPRFVASRAFPHDLRTNLDLTVADATGNICFA
                     ALVRDLVGNASATGSVTRCIRTTAPPIFYGCSMSARPARPESLGGLALALVAILARRR
                     AGQR"
     gene            1184..1954
                     /locus_tag="VFQ35_09290"
     CDS             1184..1954
                     /locus_tag="VFQ35_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930869.1"
                     /translation="MGATRGIWLVALGLLACRARTSPERLPITRAHSIRVPANDPSRS
                     CSDVQDARVCWAAEPGEGLWVGERPLPGPNAADPRKYRCTGVGDQRECRLRADLAPPF
                     RCREERCTQPLPRVPDDGEWECADLGGVVLCRGGGVPAGVARPSSDPGWRCGARRKSS
                     TGERICLDLDPDYPGKRGESFRCHYDYEGAKLTRTCEPSRAPVIGTRCESKGACPADA
                     RCIGGICLPASFNPSCWGDLDCSGDERCVYGACAPRSP"
     gene            1951..>3551
                     /locus_tag="VFQ35_09295"
     CDS             1951..>3551
                     /locus_tag="VFQ35_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930870.1"
                     /translation="MKRAVSVLLALVAASCTASGERPSPTRRLVVSAPELPSDALEGL
                     HVKPRNAVVHVERRSGLLSLELGAATKLELEAPGACPLVLDANDLPRHVRLEPRIEIT
                     APSDDVGYDSPFELSLRIRCGELENPRGTVAWESQGAPLAELVVLDQGYRLKGRTERP
                     PSRVARARVGDIVAISPSERGLTVLTARWTGSDGSVAQRSVAVRAAPRARGLPNVALG
                     ERTLLGGKNFAVIQRPPGAQATPRAWGAFTSLVPDARGFWELSSGNHRVRLLVGSYDS
                     VPLDCGRSECHSAETRAAENSPMTSAFKRLLEGPGATPGLDCALGCHTTGEVNRNDGG
                     FSHALRELAQDPGDLPGWDQLPRALRRTGGVGCLACHGPGQIPAPSARWAILRTEVCA
                     YCHDAPPRYGHVKAWRSSALAASDRDPQTRANAQCARCHTTWGFLDATAGRLVGTRMP
                     PADAEPGGIACAACHAVHDESAALPSLLRRAPIAETFSDLPESALERSSACIYCHAPS
                     ESSSPSSSLIWAGRGATDPVSLLPLL"
BASE COUNT          553 a         1246 c         1211 g          541 t
ORIGIN      
        1 gcagacggcc gcagcttcgg gtcctgtcga gagcgacgtg tgccgtcgga gtgcgcgggt
       61 gcggccgggc gcgacggaga catcgccaag ctcgagcaat gctgcatcgc gcatggctat
      121 tgctgcgtcg atagtgaaga cctcgatcac gacggcgatc gcgccgaggt acttggaaat
      181 tgcgaggacg tgagctgcga ctaatgctcc ttcgaccgaa ccaacatcgc gggccgcagc
      241 gtccggattt tccgaggtgc gcgtccgcag gcgagcgact ggtgtgtcgt atgcgcggct
      301 cccgctctat gattcgcggc acggtgccct cgacgcgatt ggcagcctcc ctggcggcgc
      361 tcgtcaccct cttcgcgcag gctacgtacg cagacgactg cgggcgcccg aacttggtgg
      421 caaccttccc aatcgacggc gccagcgccg tgccgctcaa cgcgaagctc tccgcaagct
      481 acgccgaaag cgcagactac ctcggcgaag cggtgacgct cgcttcggaa acgggcgacc
      541 gcacgctcga cgcacggttc gacgcgagcg aacgacggct catcgtcgac gagcccgcgc
      601 tcgagccgaa cacgcactac actctcgcct ggccggcgct gcgcggcatc tcgaccgccg
      661 gccatggctc gacgaagacg atttcgttca ctaccggcga cgcgctcgac gccgcggccc
      721 cggactttca gggcatcacg aagctcgcgt gggacttggt tcatccgcgc gacgagtgca
      781 cggacgatct cgagccgaga atgcggttca gcttcggctt gggagaagcg cgcgacgacg
      841 gcggcgccga gtcgctcgag ctgctcttgt tccagaccag cccgggcagc gccgacgcgc
      901 cgcggttcgt ggcgagccgc gcgttccccc acgacttgcg caccaacctg gatttgaccg
      961 tcgccgatgc caccggaaac atctgcttcg cagcgctcgt ccgagacctg gtcggcaacg
     1021 cttcggcgac aggcagcgtg acccgctgca tccgaaccac cgccccgccc attttttacg
     1081 gctgttcgat gagcgctcgg cccgcgcggc ccgagtcgct cggcggtttg gcgctggcgc
     1141 tggtcgcgat cctcgctcga cgtcgcgccg gccagcggtg agcatgggag cgacgcgggg
     1201 gatctggctc gtggcgctcg gcttgcttgc ctgccgagcg cggacgagcc ccgagcgcct
     1261 acccatcacg cgcgcgcatt cgatccgagt cccagcgaac gatccgagcc gctcgtgcag
     1321 cgatgttcaa gacgcgcggg tctgttgggc cgccgagccc ggcgaggggc tttgggtcgg
     1381 tgagcgaccg ttgccgggac cgaacgcagc cgaccctcga aagtatcgct gcaccggggt
     1441 tggggatcag cgcgaatgcc ggctgcgcgc cgacctcgct ccgccttttc ggtgtcgaga
     1501 agagcgctgc acacagccgc tcccacgggt accggacgac ggcgaatggg agtgcgccga
     1561 tctcggcgga gtagtcctgt gtcggggcgg tggagttcca gccggagtcg cgaggccgag
     1621 cagcgatccg gggtggcgct gcggtgcgcg ccgaaagtcg agcaccggcg agcgaatttg
     1681 cctcgacttg gatccagact atccgggtaa acgcggcgag agctttcgct gtcactacga
     1741 ctacgaggga gcgaagctca cgcgcacctg cgaaccgagc cgcgctcccg tgatcgggac
     1801 gcgctgcgaa agcaaaggag cctgccccgc cgatgcgcgc tgcatcgggg gaatttgcct
     1861 ccccgcgtcg ttcaacccga gctgctgggg agacttggac tgctcgggcg acgagcgttg
     1921 cgtgtacggc gcctgcgcgc cacggtcacc atgaagcgcg cggtgtcggt cttgttggcg
     1981 ctcgtagctg cgagctgcac ggcctcgggg gagcgccctt cgccaacgcg ccgtctcgtc
     2041 gtgagcgctc ccgagctgcc gagtgatgcg ctcgaagggc tgcacgtgaa accccgcaac
     2101 gccgtcgtgc acgtcgagag gcgaagtggg ctcttgtcac tcgagctcgg cgccgcaacg
     2161 aagctcgagc tcgaagcgcc gggcgcctgt ccgctcgtgc tcgacgcgaa cgacctgcct
     2221 cgtcatgtcc ggctcgaacc acgcatcgag atcacggcgc cgagcgacga cgtcggctac
     2281 gactcgccct tcgagctttc gctgcgaatt cgctgcggtg agctcgagaa cccgcgggga
     2341 acggtggcgt gggaatccca gggtgcgccc ctcgcggagc tcgtcgtcct cgaccaaggc
     2401 tatcgcctca agggacgaac ggagcgccca ccgagccgcg tcgcgcgagc gcgcgtgggc
     2461 gacatcgtcg cgatttcacc gagcgaacgc ggcctcaccg tgctgaccgc acgatggacg
     2521 ggctccgacg gaagcgtcgc gcaacgcagt gtcgcggttc gggcagcgcc gcgcgcacga
     2581 gggctgccga acgtcgcgct cggtgaacgc acgctgctcg gcgggaagaa cttcgccgtc
     2641 attcagcgtc cacccggcgc gcaggcgacc ccccgcgcct ggggagcctt tacgagcctc
     2701 gtgcccgacg cgcgcggctt ttgggagctc tcgagcggca accaccgcgt ccggctgctc
     2761 gtcggcagtt acgacagcgt cccgctcgac tgcggtcgct cggaatgcca ctcggccgag
     2821 actcgcgcgg ccgaaaacag ccccatgact tcggcgttca agcgcctgct cgagggaccc
     2881 ggtgcaaccc ccgggctcga ttgcgcgctc ggctgtcaca cgacgggaga agtgaaccgc
     2941 aacgacggcg gcttctcgca cgcgcttcgc gagctcgcac aggatccggg agacctcccg
     3001 ggctgggatc aactacctcg agcactgcgg aggacgggcg gcgtcggatg cctcgcgtgt
     3061 cacgggcccg gccagatccc cgcgcccagc gctcgttggg ccatcttgcg cacggaggtc
     3121 tgcgcgtact gccacgacgc gccgccacgc tacggtcacg tcaaggcatg gcgaagctcg
     3181 gcgctcgcgg cgagcgaccg cgatccgcaa acgcgcgcca acgcacagtg cgcgcgctgc
     3241 cataccactt ggggatttct ggatgcgacg gcaggtcgcc tcgtcggaac gcgcatgccc
     3301 cccgccgacg ccgaaccggg cggcatcgcg tgcgccgcct gccacgcggt gcacgacgag
     3361 agcgcggcgc tgcccagcct tctgcgccgc gcgcccatcg cggagacgtt ctcggacttg
     3421 cccgagagcg cgctcgagcg cagcagcgct tgcatctact gccacgcgcc cagcgaaagc
     3481 tcgagcccga gctcctcgct gatctgggcc gggcgcggcg cgacggatcc cgtttcgctc
     3541 ttgcccctgc t
//
LOCUS       DASTKS010000445        11430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_738497, whole genome shotgun sequence.
ACCESSION   DASTKS010000445 DASTKS010000000
VERSION     DASTKS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738497"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFQ35_09300"
     CDS             complement(<1..426)
                     /locus_tag="VFQ35_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9930871.1"
                     /translation="MTEYKSPGRTDSASIEVRAAPGEVYAAFADASKLMRWLPPGTMT
                     GRALEYDFRENGRYRISLSYDAATPAGVGKTEERTDVSSGRFLVLEPGRRIVQSVEFE
                     SAGDAYAGEMLMTWSFEPTAMGTKVTIRAENVPAGISQAD"
     gene            1101..2915
                     /locus_tag="VFQ35_09305"
     CDS             1101..2915
                     /locus_tag="VFQ35_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET9930872.1"
                     /translation="MNERVKGVTLSRRKHGGLETAPPAPAPRFSVVVPADRSRREALS
                     QDAPKSVSQAFVSALADLGVEHAFALFGGGIAPFCHALAESPIRLMHFRHEAGAAFAA
                     IEASLASGRLVVVVATTGPGLSNLYTGMIAARLEGAKVLFVSGGTPAAQRGRGAFQET
                     SNAASMNLLFSNGGLFNYAATLEDPAELEVALARLATGLSRPNGFIAHVCLPLPVQTA
                     RVQSSESPRIVTSAPAPDPGRSLAEIAELLSRETFVIWAGFGARHAGPAVRALAERTG
                     ARVMCSPRGKGVMPEGHPLFLGVTGLGGHSKVEASLSALKPQRTLVLGSRLGEFTSFW
                     SPELLPSEGFIHVDVDPDVFGAAYPNVPTVGVQAEVSSFLEALLAHWPAPRTEDAAPL
                     RREQPAIRPRESGLVRPSFLMAMLQREVVEKTDGIVLTEAGNSFALGTHHLRFDAPGR
                     YRVSTAFGSMGHAATGVIGAALGSRRKAWAIVGDGAMLMLSEVSTAAAYGIDAVWVVL
                     NDARYGMIAQGMRSIGWQPFETDFPRADFVAIARGMCGDGVQVERESEIQAALEAARA
                     ATGPFVIDVLIDPAELAPATKRNKSLVEQGVNGRGGGR"
     gene            2912..3619
                     /locus_tag="VFQ35_09310"
     CDS             2912..3619
                     /locus_tag="VFQ35_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930873.1"
                     /translation="MSALAAELSELEGPLEVTPGVEALAARVVTPGGEPRVRGYDVES
                     DLARHYHPSELSLLALTGELPSSASAAAFEVVQMFLAPVSVAHASTHAAVLARLCGAR
                     PSAVIAVAATALAEQAREEVAEHDALLSALVAGEPPPARFEAASDDELEASRRLRQAL
                     APCGVGSPCGAKPLKRMAELLATLVVCGLTRREQLEAAIVQARLMTTVAEAFAEHKAN
                     FNRYPSNVPRFCYEGDR"
     gene            3616..4425
                     /locus_tag="VFQ35_09315"
     CDS             3616..4425
                     /locus_tag="VFQ35_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930874.1"
                     /translation="MKSEIPSVSTRVAHWGFHEHRYLGYRVFAELLGNETFGGLTALS
                     VCGRRLSPECCAVLDDAAVALTLADPRIWPLKLTRLVAAYGSTVAAAAAGLTMLEGAQ
                     IGPWTSGAAAVVLVELDALIGDRGGDEAFVREAVLAYVKDRKFLWGFGTPYRSEDERL
                     VAFRRSLEAHGRHRLRHFALLEAVAKTLRELRGWEVNIGAGFAAACLDMGLEPNEIAP
                     LTTSLMQHMFFSNAVEGARQAAECLRKLPDELVSYRGARPRTSPRALARSE"
     gene            4614..5120
                     /locus_tag="VFQ35_09320"
     CDS             4614..5120
                     /locus_tag="VFQ35_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930875.1"
                     /translation="MTCEILAGTGAVFSVWGKPARTDMDRILENLGDAASRAGAPVTY
                     ITRVPVDAPAPEADVRQYLDSLMPRVVDYCKTYHVILEGVGFLAAVKRSVLVGIFQVR
                     WRNGTFFVHAGSNEVLEKVGTERRAGVEALLQQARTLGLLSNTPGLTSGAAGRAGERT
                     ENARHRSL"
     gene            complement(5246..6322)
                     /locus_tag="VFQ35_09325"
     CDS             complement(5246..6322)
                     /locus_tag="VFQ35_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9930876.1"
                     /translation="MEIRGISGFLGFGGVLASLLTVACGAEGPESSDRTLSAPDAEAE
                     LAATQQALFSCGLPPGPSTNVRKPSGVVGNLKVLNWAGFKAAVSYTFDDANSSQIQHY
                     SELNSLGVPLTFYLITGKVEASNAIWKQAVANGHEIGNHTKSHLSAGSAADVDAATTF
                     IQQSIGVRPWTFAAPNGDASYRSIAQSRFLVSRGVANGSVAPNDATDPFNLPTFIPAA
                     GAPAASFNNEVDAARTSGRWKTVLVHGFTGGSDAAYQPVALDQFVQSVNHTKSFGDVW
                     TDTMTKVGAYWIGQKVFSAVTPTTAGSTKTWRWTLPANFPPGKCLRITVTGGTVKQGG
                     VALPWNTHGYYEIALDTGSVSLSP"
     gene            complement(6577..7572)
                     /locus_tag="VFQ35_09330"
     CDS             complement(6577..7572)
                     /locus_tag="VFQ35_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930877.1"
                     /translation="MSTVVLAFVSLLPACGSTQNTAARPSEAPNVRTIASAPPPAPVK
                     DSTCSADHWCVVSGLPKQVTFSAVWGASANDIWIAGESGTLLHWDGSTWTEVPSGTQS
                     ALRALSGTSAKDVWAAGLDGVLLHFDGSSWSSTQKSGAPWSPATGPNERPIYTVFAAP
                     NALWAGGSGIRSFDGAKWSEPHHGSHLPTMSVWGSDAKNLWAVGLQGTVYRWEGHHWA
                     RAGNDMGPNFFAIWGSAPNDIWIVGSAGAIVHWGGETHATVASGTTNDLHAVLGFSSN
                     DVWAAGDQGAILHWQGSAWASSASPSKLGLLGIWGASAKDIWLVGENGVVLRRKL"
     gene            8010..9743
                     /locus_tag="VFQ35_09335"
     CDS             8010..9743
                     /locus_tag="VFQ35_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930878.1"
                     /translation="MQRMPRLLFSLVAVAFFVSASAARAVDVTTRAFDNGRSGWNSSE
                     TTFKNNNLGTLHLLRTFAVDEKVEAQPLVLGNVLYVFTMNNTVYRFDVTSGALLNSRH
                     LAEAIDPCVQPGQMDMWCVYHKWGIAATPVIDTATNTLYVVTFQRAPGSGDNGNREHH
                     LWSLDATSLADQKPEILVRGDADNGGKHFNVGTQKPYQKLRAGLGLLNGPGGKAVIVM
                     FSMNGENPNGPGNGFVFAYDTRGLQHQAGFTPTPAVWCTTPNGGAGGIWMAGSAPAIA
                     GNDIYFTTGNGAIGNGNYGESFVKLTYSPGVADDKGGKPSLALGDFWTAFDDNAREFN
                     DEDLGSAGVMLVPGTKALNLLGGGKDGVLYNLNRTNLGHNSWNPHFNLPFVATYLPNP
                     PNGVAGLPTSTAADPNWPIIKLDRNLHDGTPDQKMHHIHGTPVYLRMANHGNVYIWGE
                     NERVKVYNYNFDNQRIDGFRNQGTVYSSAGKPPPGGMPGGMLALSSNGTKAGSGVLWA
                     TIPKNGDANQAVVTGTFLAFDATTIVNDHELKKLFSFDYDFYSKFCPPVVANGRAYVA
                     TFGNSVLQFGL"
     gene            complement(9840..10490)
                     /locus_tag="VFQ35_09340"
     CDS             complement(9840..10490)
                     /locus_tag="VFQ35_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930879.1"
                     /translation="MLNIKVSANQPPWPKLRVVIDSNELASAEIRTRKPALYEFSLEG
                     DGAAHTISLSNLGSSNAPGSSLQITQIEFMDCTDLAGTCRDGGIYHRLDDYCSPLICD
                     SPSDCGSEFPGPRFSGTCESGRCLYPSCVNQPNAADTTVHCFSRQELAYILTNPHGFF
                     VNACPTVDSLTWGFRPVVGEGSCAEAPVCGPNSPADFGFPGNAGECCYGIARVCGV"
BASE COUNT         1993 a         3601 c         3712 g         2124 t
ORIGIN      
        1 gtccgcttgg ctgatgcccg cggggacgtt ctcggctcga atcgtcacct tggtgcccat
       61 ggcggtgggc tcgaacgacc aggtcatgag catttcgccc gcgtaagcgt cgcccgccga
      121 ttcgaactcg accgattgaa cgatgcgacg tccgggttcg agcaccagga agcgtccgct
      181 gctcacgtca gttcgctcct ccgtttttcc gacgccggcc ggcgttgccg cgtcgtaaga
      241 aagcgagatc cggtagcgcc cgttctcgcg gaagtcgtac tcgagcgccc gtcccgtcat
      301 cgttccgggc ggtagccacc tcatcagctt cgaggcatcc gcgaacgcgg cgtagacttc
      361 gccgggtgcc gctcggacct cgatggatgc actatcggtc ctgcccgggg atttgtattc
      421 ggtcacgact gcagtttgac acgacgggtt cgctttgaga gcgctcttac gcggcttcga
      481 gcctgaccgg atgcatcgac gacagccgtc tcgttcccaa cagggcgttg aagaaccctg
      541 tgttggaatg ctgaccggca ccaaggcgcc gctgcgcatc acgaaagccc atgaaatgtg
      601 aggctttcgc aggacgtttt tcataacctt gcgtgcgcgg ggtcactcgc ttttgcgtcg
      661 cgaatgaaag tcagaaaatt gccgacggtt gcatgacgcc cgcgcagcgc gaagtgcctc
      721 gagcttccgc ccgtctcgcg accggtacgc cgccggtagc gagcgatgcg gtggacgagt
      781 ctccccaaac gcgctgcgcg tgacgttgcg gaatgttccc gcttcgatac ccgagcgagc
      841 gtgctcaccg tcgacgaacg ggcgcgttcc cgcattcgtg cttcggtcac gcttcttttc
      901 aagcgcttcg cgctaccgag cgaacgccaa tattctgccg tcacgtgtct actacgaagg
      961 gttatttctc tcgacacccc attaagtgaa ggtgcgagcg gtcgttccgc ttggtgacct
     1021 cgccacaggt cgcacgacaa tcatcctcca agctgatgtc ctcgcggttt tcgcgggaag
     1081 ctcagcgctc ggtacaagcc atgaacgaac gagtcaaagg cgtgacactg tctcgccgta
     1141 agcacggcgg gctggaaacc gctcctcctg cgccggctcc acgattcagc gtcgtggtgc
     1201 ccgcggaccg tagtcggcgc gaagcgttgt cgcaggacgc gccgaaatcc gtttcacaag
     1261 cgttcgtctc cgccctggca gatttgggcg tagagcacgc atttgcgttg ttcggcggcg
     1321 gaatcgcgcc cttttgtcat gctctcgcgg aaagcccgat ccggctcatg cacttccgtc
     1381 atgaggctgg cgccgcgttc gcagcgatcg aagcgtcgct cgcgagtggg cggttggtcg
     1441 tcgtcgtggc tacgacgggg ccggggctca gcaatctcta cacggggatg atcgcggctc
     1501 ggctcgaagg agcaaaggtg ttgttcgttt ccggggggac gccggcagcg caacgggggc
     1561 gcggcgcgtt tcaagaaact agcaatgccg cgagcatgaa tcttttgttc tcgaacggcg
     1621 gcctcttcaa ttacgccgca acgctcgaag acccggcaga gctcgaggtc gcgttggcgc
     1681 ggttggcgac ggggctctcg cgcccgaacg gcttcatcgc tcacgtgtgt ctacccctac
     1741 cagtgcaaac cgcccgcgtt cagagttcgg aatctccgcg catcgtcacg agcgcacctg
     1801 cgccggatcc cggccgcagc ttggccgaaa tcgcagagct cttgtcgcgt gaaactttcg
     1861 tgatttgggc tggcttcggg gcgcggcacg ccgggcccgc ggttcgcgcg ctcgccgagc
     1921 gcaccggggc gcgagtcatg tgctctccgc ggggcaaagg agtcatgccc gaaggtcacc
     1981 cgttgttcct gggagtcacg gggctcggcg ggcattccaa agtcgaggcc tcgttgagcg
     2041 cgctcaagcc gcagcgcacg ttggtgctcg gctcgaggct cggcgagttc acgtcgtttt
     2101 ggtcgcccga gctgctgccg agcgaaggct tcatccacgt cgacgtggat cccgacgtat
     2161 tcggcgcggc ttatcccaac gtgccgaccg tcggcgtaca ggccgaagtc tcgagcttct
     2221 tggaggcgtt gctcgcgcac tggccggcgc cgcgcaccga agacgcggct cctttgcggc
     2281 gcgagcagcc tgcgatccgc ccgcgagaga gcggcctcgt gcggcccagc ttcctcatgg
     2341 ccatgctcca gcgcgaggtc gtcgagaaga cggacggaat cgtgctcacg gaggcgggga
     2401 actccttcgc gctcgggacg caccaccttc gcttcgatgc gccgggtcgt taccgggtga
     2461 gcacggcgtt cggctccatg ggccacgccg cgaccggggt gattggcgcg gctctcggca
     2521 gtcgccgcaa ggcttgggca atcgtcggtg atggcgcgat gctcatgctg agcgaggtga
     2581 gcaccgccgc ggcatatgga atcgatgcag tgtgggtggt gttgaacgac gcgcgctacg
     2641 gcatgattgc acagggcatg cgttccatcg ggtggcagcc cttcgagaca gatttccccc
     2701 gcgccgactt cgtcgcgatc gcacggggaa tgtgcggtga cggggtgcag gtcgagcgcg
     2761 aaagcgagat ccaagccgct ctcgaggcgg cacgagccgc cacgggtccg ttcgtcatcg
     2821 acgtgctcat cgacccggct gagctcgcgc cggctacgaa gcgcaataaa agcttggtcg
     2881 agcaaggcgt gaacggtcgc ggaggtggca ggtgagtgcg ttggcggcgg agctcagcga
     2941 gctcgaaggc ccgctcgaag tcacgcccgg tgtcgaagcg ctcgcggcgc gtgtcgtgac
     3001 gcccggcggc gagccgcgcg tgcgcggtta cgacgtcgag tcggatctgg cgcgacacta
     3061 ccatccgagc gagctctcgc tgctggcgct cacgggcgag cttccttcga gcgctagcgc
     3121 cgcggcgttc gaggtcgtgc agatgttcct cgcaccggtt tcggtcgcgc acgcttctac
     3181 tcacgctgcc gtgttggccc gcctttgtgg tgcgcgtccg agcgccgtca tcgccgttgc
     3241 ggccaccgcc ctcgcggagc aggccaggga agaagtggca gaacacgatg cgctgctgag
     3301 cgccttggtc gcgggcgagc caccccccgc gcgcttcgag gccgcatcgg acgatgaact
     3361 cgaggcaagt cggcgcttgc gccaagctct cgcgccctgc ggcgttggct cgccgtgcgg
     3421 agccaagcca ctgaagcgaa tggcggagct cctcgccaca ttggtcgtct gcggcctcac
     3481 gcgtcgcgaa caactcgagg cggcgatcgt gcaggcgcgc ctcatgacca cggtcgcgga
     3541 agcgtttgcc gagcacaagg ccaacttcaa tcgctatcca tcgaacgtgc cgcgcttttg
     3601 ctacgagggg gaccgatgaa gagcgaaatc ccgagcgtct ccacgcgggt tgcgcactgg
     3661 ggcttccacg agcaccggta tcttggctat cgcgttttcg ccgagctctt ggggaacgag
     3721 accttcggtg gactcacggc cttgtccgtt tgcgggcgtc gcttgtcgcc cgagtgctgc
     3781 gccgtcctcg acgacgccgc ggtggcactc acgctcgccg atccgcgcat ctggccgctc
     3841 aagctcacgc gcctggtagc ggcgtacggc tcgaccgtcg ccgccgccgc ggccgggctg
     3901 acgatgctcg agggcgcgca gattgggccg tggacgagcg gcgcggctgc ggtcgtgctc
     3961 gtggagctcg acgccttgat cggcgatcgc ggcggcgacg aagcattcgt gcgtgaagcc
     4021 gtgctcgcct atgtcaaaga tcgcaagttc ctctggggct ttggcacgcc gtatcgctcc
     4081 gaggacgagc gcttggtcgc gttccgtcgc tcgctcgagg cgcatggccg tcatcgactg
     4141 cggcactttg cgctgctcga ggccgtcgcg aagaccctgc gtgagctccg cgggtgggaa
     4201 gtgaatatcg gtgcaggctt cgcggccgct tgtctcgaca tggggctcga accgaacgaa
     4261 atcgcgccgc tcaccacttc gctgatgcag cacatgttct tcagcaacgc ggtcgagggg
     4321 gcccgacaag ccgccgagtg cctccgcaag ttgcctgacg agctggtctc gtatcgaggg
     4381 gccaggcctc gcacgagtcc gcgcgcgctc gctcgctcgg agtagtacga aggctgcgag
     4441 gtagcgcgcc gtcggtgaag gtggcgcaac gaccccggcg caactaccga tctgggcacg
     4501 gctcccgagc gacggcgaaa atcgctaaca tttcgcgcag gcggtcgttc actaccgaga
     4561 acattctgct cgccgcggtc gcgcggcgcg ttgctgggaa ggaacgacag cgaatgactt
     4621 gcgagatcct cgccggaacg ggagctgtgt tttcggtctg gggcaagcct gcccgcacgg
     4681 acatggaccg aatcctcgag aacctaggcg acgctgcttc gcgcgccggc gctccggtca
     4741 cgtacatcac tcgcgttccc gtggatgcac ccgctccgga ggcggacgtt cgccagtacc
     4801 tcgactcgct catgccgcgc gtggtcgatt actgcaaaac ctatcacgtg atcctcgaag
     4861 gcgttgggtt cctggccgcg gtgaagcgca gcgtgctcgt cggcatcttt caggtccgct
     4921 ggaggaacgg caccttcttc gtgcacgcgg gcagcaacga agtgctcgaa aaggtcggta
     4981 cagagcgccg agcgggggtc gaagcgctgc ttcagcaagc tcgcaccctg gggctcctct
     5041 ccaacacacc ggggttgacg agcggagccg cgggacgagc tggcgagcgc acggagaatg
     5101 cgcgccatcg ctcgctctga gctaccctca cccggcaaaa ctacttttcc gcggtcgtgt
     5161 tccacggcgc ctgcgaccac gcaaaattca ttggagcgca gcgccatcga ctacgctcca
     5221 acgagatcgg ccctgcgaga cgcggtcagg gggacaacga cacggagccg gtgtccaggg
     5281 cgatttcgta atagccgtgc gtgttccacg gcaacgcgac gccgccttgc ttgaccgtgc
     5341 cacccgtgac cgtgatgcgc aggcacttgc ccggtgggaa gttcgcgggc agcgtccagc
     5401 gccaggtctt ggtcgagccg gctgtggtcg gcgtaacggc ggagaagacc ttctgtccaa
     5461 tccagtacgc gccgaccttc gtcatcgtat cggtccaaac atcgccgaac gatttcgtgt
     5521 gattcacgct ctggacgaac tgatcgaggg cgacgggttg atatgcggcg tcggacccgc
     5581 ccgtgaaccc gtgcacgagg acggtcttcc agcggccaga cgtgcgcgct gcgtcgactt
     5641 cgttgttgaa cgaagcggcg ggcgcacccg ccgcaggaat gaacgtcggc aaattgaagg
     5701 gatcggtggc gtcattcgga gcgactgagc cattggcgac tcctcgactc accaggaagc
     5761 gcgactgcgc gattgaacga tagctcgcgt cgccgttcgg agccgcgaaa gtccagggac
     5821 ggacaccgat cgattgttgg atgaaagtgg tggccgcgtc gacgtctgcg gcgctgcccg
     5881 cgctcaggtg gctcttcgtg tgattaccga tctcgtgtcc gttcgccacg gcttgcttcc
     5941 agatcgcgtt cgacgcttcg accttgcccg tgatgagata gaaggtcagc ggaacgccga
     6001 gcgaattcag ctccgagtag tgctgaattt gcgacgaatt ggcgtcatcg aacgtgtagg
     6061 agaccgccgc tttgaacccc gcccaattca acactttgag gttacccacc acccccgaag
     6121 gtttgcggac gttcgtgctc ggccccggag ggagcccgca ggaaaacaat gcctgttgcg
     6181 tggccgcgag ctccgcttcc gcatcaggcg cgctgagtgt gcgatcgctg ctctcgggcc
     6241 cttcggcccc acatgcaacg gtgagaaggg acgcgagcac accaccaaac cccaagaaac
     6301 cagaaatacc gcgaatttcc atcaacctcc accctttcgc gaggcagact accagagact
     6361 tctaacgagt ggggtcagaa actttgcggg cgccgtggtg ttggctgcgc tcggaggtgg
     6421 aggtgcctgt ctcgtgatgg cgcgcccgaa gctgcggcgg acgggctcgt cgccgctctc
     6481 ctccgctcac tttagtggcg tccggtgcta actccccaca cgccccctcg cggcggtctc
     6541 ggcttgctcg cgactgactt atccattcgc gggcactcag agtttccgtc tcagcacgac
     6601 gccgttctcg ccgacgagcc aaatgtcttt cgcgctcgcg ccccaaatgc cgagcaagcc
     6661 gagcttcgat ggacttgccg agctcgccca cgccgagcct tgccaatgca gaatcgcgcc
     6721 ttgatctccc gcggcccaga catcgttcga gctgaagccg agcaccgcgt gcaagtcgtt
     6781 ggtcgtgccg ctcgcgaccg ttgcatgcgt ctcgcctccc caatgcacga tggcgccggc
     6841 gctgccgacg atccaaatgt cgttcggcgc gctcccccaa atcgcgaaga agtttggccc
     6901 catgtcgttc ccggcgcgcg cccagtgatg gccttcccat cgatagaccg tgccctgcag
     6961 ccccaccgcc caaaggttct tcgcgtccga gccccacacg gacatggtgg gtaaatgcga
     7021 gccgtggtga ggctcgctcc acttcgcgcc atcgaacgag cgaattccag acccgcccgc
     7081 ccaaagcgcg ttgggcgccg cgaagaccgt gtaaatgggc cgctcgttgg gaccagtggc
     7141 gggtgaccaa ggcgctccgc tcttttgcgt ggaggaccag ctcgagccgt cgaagtgcag
     7201 cagcaccccg tcgaggccgg cagcccaaac gtctttggca gaagtgccgc tcaacgcgcg
     7261 aagggcgctc tgcgtgccgg aaggaacttc ggtccaagtc gagccgtccc aatgcagcaa
     7321 ggtgccgctt tcaccggcga tccaaatatc gtttgctgaa gcgccccata ctgcgctgaa
     7381 agtgacctgt ttggggagtc ccgaaacgac gcaccagtgg tcggcgctgc acgtcgagtc
     7441 tttcaccggg gcaggcggtg gggccgacgc gatggtgcgc acgttcggtg cttcgctcgg
     7501 acgggcggca gtgttttgtg tggagccgca agcgggcagg agggatacga acgcgaggac
     7561 gacggtgctc aagcggttac gcgctacgcg ggcgcaccgg tcgacgaacg atcgggtcga
     7621 gactgtcgcg gtcgccacgt gatgcggtct gagcgtcgcc atgcgtggca tcgtagatca
     7681 cgaatcggcc gcattgggtc gagaacgtcc gtgctcgtga aatcgcacac tggaaaagtt
     7741 actacgttcg acgccgcatg tgtcgaagtc cgctcgactc ggtgtgctga atagggattg
     7801 cattgcgccc ggccatgcgg agctctcggg tcgaattcgc cgtccttgct ccgaaacttc
     7861 gcggaagcaa agagccgccg gcgggtgacg agattcgaac gcggcgatgc gacttgcgaa
     7921 gtgggtcgat gttcgtgaga atgcgcgagg cctctcggac ccggtgggct tcgacgctcg
     7981 ttcaagcgcg cagccgtggg agtttcagca tgcaacgaat gccgcgcctt ttgttttcgc
     8041 tggtcgcagt cgcgtttttc gtcagcgcct ccgctgcgcg cgccgtggac gtcacgacgc
     8101 gtgctttcga caatggtcgc agcgggtgga acagctcgga gacgactttc aagaacaaca
     8161 acttgggcac gttgcacttg ctgcgaacgt ttgccgtcga cgagaaggtg gaggcgcagc
     8221 cattagtgct cggaaacgtg ttgtacgtct tcacgatgaa caacacggtg tatcgcttcg
     8281 acgtcacgag cggagctttg ctcaactcgc gtcacctggc cgaagcaatc gacccgtgcg
     8341 ttcagccggg ccaaatggac atgtggtgcg tttatcacaa gtgggggatc gccgcgacgc
     8401 cggtgatcga caccgctacc aacactctct acgtggtgac gtttcagcgc gccccggggt
     8461 cgggagacaa cggcaatcgc gagcatcacc tctggtcact cgacgcgacc tcgctcgcgg
     8521 atcaaaagcc ggagattttg gtgcgtgggg acgcggacaa cggcggcaag cacttcaacg
     8581 tcggaacgca gaagccatac caaaagctac gcgccggtct cggtttgctg aacgggcccg
     8641 ggggcaaagc ggtgatcgtg atgttctcga tgaacggcga gaacccgaac ggccccggta
     8701 acggcttcgt attcgcgtac gacacgcggg ggttgcagca tcaggccggt ttcaccccga
     8761 cgcccgcggt ttggtgcacg acgccgaacg gcggcgcagg agggatttgg atggctggtt
     8821 cggcgcccgc gatcgccggc aacgacatct atttcactac cggaaatggc gcgatcggta
     8881 acggcaacta cggagagagc ttcgtcaagc tcacgtactc gcccggcgtc gcggacgaca
     8941 aaggcggcaa gccgagcctc gctttgggtg acttttggac tgccttcgac gacaacgccc
     9001 gcgagttcaa tgacgaagac ttggggtcgg ctggcgtcat gctcgtgccg gggacgaagg
     9061 cgttgaacct cctcggcgga ggcaaggacg gtgtgctcta caatttgaat cgcacgaacc
     9121 tcggccacaa ctcgtggaat ccgcatttca atttgccgtt cgtagcgacg tacctgccaa
     9181 atcctccgaa cggcgtggct ggtcttccta cctccacggc tgccgatccg aattggccca
     9241 tcatcaagct cgatcggaac ctgcacgacg gaacgcccga tcagaagatg caccacatcc
     9301 acggcacgcc tgtctacctt cggatggcaa accacggaaa cgtgtacatc tggggcgaga
     9361 acgagcgagt caaagtctat aactacaact tcgacaacca gcgtatcgac ggcttccgca
     9421 accagggcac cgtttactct tcggcaggca agccacctcc tggaggcatg cccggcggca
     9481 tgctcgcgct ctcttcgaac gggacgaagg ccggcagcgg cgtcttgtgg gcgacaattc
     9541 ccaagaatgg cgacgcgaac caagcagtcg tgaccggcac gttcctcgct ttcgacgcga
     9601 ccacgatcgt gaacgatcac gagctgaaaa agctcttttc gttcgactac gacttctatt
     9661 cgaagttctg tccccccgtg gtcgcgaacg ggagggccta cgtcgcgacg ttcggcaatt
     9721 ccgttctaca gttcgggcta tagctgcacg caagctcgct cgcccgccgt gaccgaggtc
     9781 cgacgggcgg ccgagtgtcc gcgcttggtg gcggggtaac ggcagcagtg cgcgcgacgc
     9841 tatacgccgc acacccgcgc gatcccgtag caacactcgc ccgcgttgcc cggaaagccg
     9901 aagtccgcgg ggctgttcgg cccgcaaacg ggcgcttcgg cgcagctgcc ctctccgact
     9961 accgggcgaa agccccacgt cagtgagtca acggtgggac atgcgttgac gaaaaagccg
    10021 tggggattag tcagaatata cgcgagctcc tgcctcgaga agcaatgaac ggtcgtgtcc
    10081 gctgcgttcg gttgatttac gcaactcggg tagaggcacc gcccgctttc gcaagtgccg
    10141 ctgaaacgcg ggccggggaa ctcgctcccg caatcgctcg gactatcgca gatgagtggg
    10201 gaacaatagt catcgagccg gtggtagatg ccaccgtctc tgcaagtacc tgcgagatcg
    10261 gtacagtcca tgaactcgat ctgcgtgatt tgaagcgagg agcccggggc gttcgagctg
    10321 ccgaggttgc tgagcgaaat ggtgtgcgcg gcgccgtcac cctcgagcga gaattcgtag
    10381 agcgcgggtt tgcgggtccg gatctcggcg ctggccaact cgttagaatc gatgacgacc
    10441 cgcagcttcg gccaaggcgg ctggtttgcg ctcaccttga tgttgagcac cgccggcgca
    10501 ggcggaaaga catgctccag cgtaaccgct ttttcgggcc ctagcgtgta ctcttgtccg
    10561 tcgcgcaggt cgtgaagcga gctagcttct gtgcacgtca ggcgtgacct gcaaacgcca
    10621 gcgaaacagt gctcgtcctc ggcgcaggcg tgttcgcaac tgccgcaatg cgcggcgtcg
    10681 aaatgttcat cgacgcaagc ctgcccgcac agaagataat tcggaaactc gcaactcgga
    10741 gattccgtgc cacccgatcc ggttgagccc gagctgccag aggcactcga gccgcctttt
    10801 gcgtttgacg tcgttcccgc gctcgggttg ccgccgcttg cgagttggtt ggtgggagcg
    10861 ccgccagttc ccgcgtgggc ggcctcggtc gtgcgtccac aagcgctcag cgccaaggtc
    10921 aggccgactg tgactcgctc gcgaaggcgt gcgccagacc ggataggcgt cgggccagac
    10981 cgattcggca ttgaatcagg ctcgcatggc ctccgatccc cggcaatccg cgggtggcga
    11041 tattggtcat cgggcctctc cccaacttcg ataaggaagc cgccttcggg aatgacctgg
    11101 aggagccaac cccgaaagtg gcgccgcggc gctcgcgagc gctgaggctc cggcccgcgg
    11161 gtctcagcgc ggctgatacg taattcgtca tgataatcga ggacggggat aagtttttga
    11221 gactcgagtc gtttgctgct gtcgatgcac tggtggttgg ctacggcaac acgcagggaa
    11281 gggcgttggc gagcggcact ccgttgctgt ccggtggtcc tcctgctcgc gggcgggtgc
    11341 ttctatgact cggacgatcg ttgcgactcg acgcagcatt tcgacgaggc ggcgggtgtt
    11401 tgcatttgtc gaccgggcac gatcccgggc
//
LOCUS       DASTKS010000446         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_71419,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000446 DASTKS010000000
VERSION     DASTKS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71419"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFQ35_09345"
     CDS             <1..1049
                     /locus_tag="VFQ35_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930880.1"
                     /translation="GKPDPELFLKGRPMSRLSRLVRSPRFKGWLRVFACLTVFQVIVA
                     AVLLRTARARGAATLQEGGLKLLEHVGPDLVGEAVEATINGQRLSIASQFSPLPIPEV
                     LDRFERHCQANSGGLAEEVSKLPEGRALGQLPSEFRDPSRWLTSRQIDASGKVGQVVC
                     LVRRTSGGGVRGLVDQIMRFVDTGDLAELGGARYVVARREKSDTDTHVLAIWSDGSFN
                     IPNMFPAEGDAPGRDSPYVPRPFEARRVFSAEIEGRAYALRMYDSRRSNADVLRQYAE
                     ALPKSGWLPHPMPPLRHGEGEGEDLGEHFSAYTKGSAAVVVVVEDTPDDLTGVTLIEM
                     GGRGFAQIAGENQP"
     gene            1046..1867
                     /gene="cpaB"
                     /locus_tag="VFQ35_09350"
     CDS             1046..1867
                     /gene="cpaB"
                     /locus_tag="VFQ35_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9930881.1"
                     /translation="MNRRALLVSVVLALLGRALLVLYTRRFEHDMSGGEAVSLLTVLK
                     PVERGKVITDDVIGVRSVPLAYVEDRAIKAAERSKVLGLQTAVPLYPQQLLMWTDLAI
                     TTEDRDLSSLVQPGKRGFTVRAGTADDTRGNALIRPGDYVDVIATMRNEADGVTNPDP
                     ASVVLLQRVLVLAVGSETQATGADANKRDSAYAQRDKLLTLSLTLSEVQLVTLALDRG
                     QLSVAVRPPEDPSVQTDVPDMKASALFDTKLRANVQRIVRPNNEGPVRIEAASLR"
     gene            1864..>3109
                     /locus_tag="VFQ35_09355"
     CDS             1864..>3109
                     /locus_tag="VFQ35_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET9930882.1"
                     /translation="MRHRCLRVLLAALLVTGGAFAQREAGQHSDALIERSEELNLAVG
                     ETKSLSAVDVKSFSEGIRGIADVRLTPDSSQFIVVGQKSGATTLLLIKKDGTQLTYNI
                     NVFSRSPASVERELSQLLDGTPGVRMRRVGARFFIEGGVSNEPALKRIEQIAALFPGQ
                     VESLVVLGGAAAERRLNLRIDFMFVQFDRSKGYQVGVSWPGRIGGDRRFTLGGEFDFL
                     SGVVGAARATVVDQPLPALDLAATKGWAKVLKYSTVVTANGTEANFSSGGEQNYVISS
                     GFGAALTQVRFGTDVKVLPRFDPATRELEVQLAAEVMDLTPPVAAGTDLPGRNVSKLS
                     TLVSLKLGQSIVLSGIRTRSQRHSVSGLPLLSEIPILGVFFGSHGDSQEEVEGAIYVV
                     PSIIEAVPKSAQEIVDEALREYK"
BASE COUNT          550 a         1022 c         1016 g          521 t
ORIGIN      
        1 gcggcaaacc tgatcccgag ctttttttga agggaaggcc gatgtcccga ctatctcgac
       61 tcgtacgctc gcctcgcttc aagggctggc tccgtgtctt cgcctgcctc accgtatttc
      121 aagtgattgt ggcggcggtt ctactgcgca cggcccgcgc gaggggcgcc gcgacgctcc
      181 aggagggcgg gctcaagctg ctcgagcacg tcggccccga tctcgtcggc gaggcggtcg
      241 aggcgaccat caacgggcag cgcctgtcca tcgcttcgca gttcagcccg ctgccaattc
      301 ccgaggtgct cgaccgattc gaacgccact gccaagccaa ttccggaggc ctcgccgagg
      361 aagtgtcgaa gctccccgag gggcgcgcac tcggccagtt gccgtccgag tttcgcgatc
      421 cttcgcgttg gctcacgtct cgtcaaatcg atgcttccgg caaagtcgga caggtggtgt
      481 gcctcgtgcg acgcacgagc ggcgggggcg tgcgtggcct cgtcgaccaa atcatgcggt
      541 tcgtcgacac cggagacctg gccgagctcg gcggcgctcg ctacgtggtg gcgcgccgcg
      601 aaaagagcga caccgacacg cacgtcctcg cgatttggag cgacggctcc ttcaacatcc
      661 ccaatatgtt ccccgccgaa ggcgacgccc ccggccgcga ctcgccctat gttccgcggc
      721 cattcgaggc gcggcgcgtc ttcagcgcag aaatcgaagg acgcgcctac gcgctgcgca
      781 tgtacgactc gcggcgcagc aacgctgacg tactgaggca gtacgccgaa gccttgccga
      841 agagcggctg gttaccgcac ccgatgccgc cgcttcgcca tggcgagggc gaaggcgaag
      901 atctcggcga gcacttctcc gcctacacga aaggtagcgc cgcggtggtg gtcgtcgtcg
      961 aggatacccc ggacgacctc accggggtca cgttgatcga gatgggcggg cgcggcttcg
     1021 cccagatcgc cggagagaac caaccatgaa tcgtcgtgcg ctgctcgttt ctgtcgtctt
     1081 ggcactgctc ggcagggcgc tgctcgtgct ctacacgcgg cgcttcgagc acgacatgtc
     1141 gggcggagaa gcggtctcac tgctcacggt gctgaagccg gtcgagcgcg gcaaagtcat
     1201 caccgacgac gtgatcggag ttcgctcggt ccccctcgcg tacgtcgaag atcgcgccat
     1261 caaagccgcg gagcgctcga aggtgctcgg ccttcaaacc gcggtcccgc tctaccccca
     1321 gcagctactg atgtggacgg atctggccat caccaccgaa gaccgcgact tgagttcgct
     1381 ggttcagccc ggcaaacgcg gcttcacggt gcgggccggc accgccgacg acactcgcgg
     1441 caacgcgctg attcgtcccg gcgactacgt ggacgtcatc gcaacgatgc gcaacgaagc
     1501 cgacggcgtg acgaaccccg atccggcgtc ggtcgtgctg ttgcaacgcg tcttggtgct
     1561 ggcagtggga agcgaaacgc aagcgacagg cgccgacgcc aacaagcgag acagcgcgta
     1621 cgcccagcgc gacaaactcc tcactctgag cctcactctg agcgaagttc agctcgttac
     1681 gctggcgctc gaccgcggac agctctcggt cgcggttcgg cccccggaag accccagcgt
     1741 tcagacggac gtccccgaca tgaaggcatc ggcgctgttc gacaccaagc tccgcgccaa
     1801 cgttcagcgc atcgtccgac ccaacaacga aggcccggtg cgcatcgagg cagcgagctt
     1861 gcgatgagac accgctgctt gcgggtactg ctcgcggccc tgctcgtcac cgggggcgcc
     1921 ttcgcccaac gtgaagcggg gcagcattcc gacgcgctca tcgagcgaag cgaggagctg
     1981 aacctcgccg tcggcgaaac gaagagcctt tcggcggtgg acgtaaagag cttctccgaa
     2041 gggattcgcg ggatcgccga cgtgcgactc accccagaca gctcgcagtt catcgtcgtc
     2101 gggcagaagt ccggcgcgac gacgctgttg ctcatcaaga aggacggcac gcagctcacc
     2161 tacaacatca acgtcttctc gcgctcaccg gccagcgtcg agcgcgagct ctcgcagcta
     2221 ctcgacggca cgcccggggt gcgcatgcgc cgcgtcggcg ctcgcttctt catcgagggc
     2281 ggcgtgagca acgagcccgc tttgaagcga atcgagcaaa ttgccgcgtt gtttccgggt
     2341 caggtggagt cactcgtcgt cctgggcgga gccgcggcag agcggcggtt gaacctgcgc
     2401 atcgacttca tgttcgtgca gttcgatcgc agcaagggct accaagtcgg ggtatcttgg
     2461 cccgggcgca tcggcggtga ccggcgcttc accctcggcg gggagttcga cttcttgtcg
     2521 ggggtggtgg gcgcggcgcg cgcgaccgtc gtggatcaac cgctgccggc actcgatctc
     2581 gccgcgacga agggctgggc gaaggtgctg aagtactcaa cggtcgtaac cgccaacgga
     2641 accgaagcca atttttcgag cgggggagag caaaactacg ttatctcgag cgggttcggc
     2701 gcggcgctga ctcaggttcg gttcggcacc gacgtgaagg tgttgccgcg cttcgatccg
     2761 gcgacgcgcg agctcgaggt gcagttggcg gcggaggtca tggaccttac accccccgtc
     2821 gcggccggaa cggatctgcc aggccgcaac gtctcgaagc tgagcacgct cgtatcgctg
     2881 aagctcgggc agtcgatcgt cttgtcgggg atccgcacgc gctcgcagcg gcactcggtg
     2941 agcgggctgc ccttgctcag cgaaataccg attctgggtg tgttcttcgg ctcgcacggc
     3001 gacagccaag aagaggtcga aggcgcgatc tacgtcgtcc cgagcatcat cgaggccgtc
     3061 ccgaagagcg cgcaagagat cgtcgacgag gcgctgcgcg aatacaaac
//
LOCUS       DASTKS010000447         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1632378, whole genome shotgun sequence.
ACCESSION   DASTKS010000447 DASTKS010000000
VERSION     DASTKS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632378"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFQ35_09360"
     CDS             <1..415
                     /locus_tag="VFQ35_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930883.1"
                     /translation="GAEAVLQLRAIRASKDFDAYWAFHLERERARNHASRYEDGKIPD
                     PLPAPKACLKRVRRSNSLHYPSAEKSRTQAVCRSALAFDVVDVVRVTRMLKSAIKTPP
                     PSADNGNTKLVQLTLPRFARPEHQFQTRPGSKEGK"
     gene            668..1819
                     /locus_tag="VFQ35_09365"
     CDS             668..1819
                     /locus_tag="VFQ35_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9930884.1"
                     /translation="MVRQAHARRAHESALSRSAQAHGRARSVGVGKTFIAQALGHIAC
                     RHGYHVRFTRADEMLRRLRQSRLDNSRDAEMIALTSVDLLVLDDFALEPMSKEESKDV
                     YQLFLERTGRASMIVTSNRDTAEWLAMFDDVLLAQSAVDRFKNAAYDFVIEGESYRRR
                     LKPKLDEANPPPASPAKTPASTRALGHAAGADPGASAGSRRLRGGGMPLQKSGAASTA
                     NASNAIVDTAPDSGHSRSRSFSPSLWRHHPAKQVAAWGANWQSANCGTIWRGDWSRNR
                     SLVKTSSTRRRCSIVRAFGGVVLGPARPSSGGPRQPPPLELRRHPCVRRRFVPTVAQH
                     ARAFPSCAAAVRNRRSRGNARTPVFCAEVSLSSGRAGRHAGCSSASACD"
     gene            1810..>2143
                     /locus_tag="VFQ35_09370"
     CDS             1810..>2143
                     /locus_tag="VFQ35_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930885.1"
                     /translation="MRLSITIQRSSSVVLAVALAAAACGSNASDDDSATDTHTHDGAG
                     TGGGMPTGGSGGTAGTAGSTATGGSAGSAGTSGSAGTGGGPTQCFGHPTADEHPSAFG
                     QCLYSYCCG"
BASE COUNT          383 a          753 c          667 g          340 t
ORIGIN      
        1 cggagcggag gccgtgctcc aactccgcgc catccgcgcg tccaaggact tcgatgccta
       61 ctgggccttt cacttggaac gagagcgcgc tcgcaatcac gcttcacgct acgaagacgg
      121 caagatccct gacccccttc cggctcccaa ggcgtgtctc aagagggttc ggcgatcgaa
      181 ctcgctccat tatccgtcgg cggaaaagag ccgcacccaa gcggtttgcc ggagcgcgct
      241 cgcattcgac gttgtcgacg tcgtccgcgt cactcgcatg ctcaagtcag caattaagac
      301 gccgccacct tcggccgaca acggtaacac aaagctcgtg cagctcacgc tgccgcgctt
      361 cgctcgcccc gagcatcagt tccagactcg gcccggctcg aaggagggga agtgatggac
      421 acgacctcgc tcagccacga gctcgtctcc gcactcaagc gcctcaagct cggtcgcatc
      481 gccgacacgc tccccgacag actcgtgctc gccgaaaagc aggacatgac gttcgaggac
      541 ttgctcctgc tcgtcatcac cgacgagatc gcgcgccgcg acaacgcggc ctcagagatc
      601 cgcgcagttc aggccggatt gcacccgaaa gtgcgcctcg agctctggga caaaaccgcg
      661 caaaagtatc gttcgacaag cgcatgctcg ccgagctcac gagtctgcgc tttctcgaag
      721 cgcacaggca cacggtcgtg ctcggtccgt cggcgtcggc aagactttca ttgctcaggc
      781 gcttggtcac atcgcgtgca ggcacggtta ccatgtccgc ttcacgcgcg ccgacgagat
      841 gctgagacga ctccggcaaa gccgtctcga caactcgcgg gacgccgaga tgatcgcact
      901 cacctccgtc gacctgctgg tgctcgacga cttcgcgctc gaacccatga gcaaggaaga
      961 gagcaaggac gtctatcaac tcttcctcga gcgcaccggc cgcgcctcca tgatcgtcac
     1021 cagcaaccgc gacaccgccg agtggcttgc gatgttcgac gacgtcctgc tcgcgcagag
     1081 cgccgtcgat cgcttcaaga acgccgccta cgacttcgtc atcgaaggcg agtcgtaccg
     1141 gcggagactc aaacccaagc tcgacgaagc caatccgccg cctgcgtcgc cggcgaaaac
     1201 gcccgcctcc acccgcgcgc tcgggcacgc cgcaggcgct gaccccggag cctccgccgg
     1261 ctctcgccgg ctccggggtg gcggcatgcc tctgcaaaag tcgggtgcgg cgtcgacggc
     1321 gaacgcctcg aacgccatcg tcgacaccgc gcccgactcg gggcactctc ggagccggtc
     1381 gttcagcccg tccctctggc ggcatcatcc tgcaaagcag gtggcggcat ggggtgcaaa
     1441 ctggcaatcg gccaattgcg gcacgatttg gcgcggagac tggtcgcgaa atcgctcgct
     1501 cgtgaagacg tcgagcaccc gccgacgctg ctcgattgtc agggcgttcg gcggcgtcgt
     1561 cttggggccg gcgcggccgt cctcgggcgg tccgcgccag cccccccccc tcgaactccg
     1621 ccgccatccg tgcgttcgtc gacggttcgt gccgaccgtt gcgcaacatg cgcgagcgtt
     1681 cccgagttgc gcagccgcgg tgcgcaaccg acggtcccgg ggtaacgccc gcacgccggt
     1741 attttgcgcc gaagtgagcc tttcgagcgg acgcgctggg cggcacgcag gttgctctag
     1801 cgcttcggca tgcgactaag catcacgatt cagcgttcga gcagtgtcgt tcttgccgtt
     1861 gccctagccg cagcggcttg cggcagcaac gcttcagatg acgactcggc cacggacact
     1921 cacacgcatg acggcgcagg gacgggtgga ggcatgccaa ccggcggcag cggaggcacg
     1981 gcgggaaccg ctggaagcac ggcaaccggc ggtagcgcgg gcagcgcggg gacgagcgga
     2041 agcgcgggga ccggcggcgg accgacgcag tgcttcggcc atcccaccgc cgacgagcat
     2101 ccgagcgcgt ttgggcagtg cctctattcg tactgttgcg gct
//
LOCUS       DASTKS010000448         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_963175, whole genome shotgun sequence.
ACCESSION   DASTKS010000448 DASTKS010000000
VERSION     DASTKS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963175"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..820)
                     /locus_tag="VFQ35_09375"
     CDS             complement(203..820)
                     /locus_tag="VFQ35_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9930886.1"
                     /translation="MRNTNLVKATSFWKRLWLGTFIAVFALLSAGCGYNEVVERDEDV
                     KAAWAEVQNQYKRRADLVPNLVKVVKGAGQFEQDTLTKVVEARSKVAGIKVDASTIDD
                     PAKLKAFEQAQGELGGALSRLLVVAERYPELKATDAYRDLQAQLEGTENRIAVARKRY
                     IDGVAEYNKVVARFPTSIGASMRGMKSRPTFEGTPGLEKAPDVNL"
     gene            complement(945..1403)
                     /locus_tag="VFQ35_09380"
     CDS             complement(945..1403)
                     /locus_tag="VFQ35_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9930887.1"
                     /translation="MSEHRTRIEWQRTGDDFSLASFSRNHEVSFGTGQSLKLSATEEY
                     RGDANRVNPEELLVSALSSCHMMTFLALAARGGLLVDQYSDDAVGYLEKDGGGRLAVT
                     RVILRPRVRFGSSKPAPEELRALHERAHRGCFIANSVKTEVHVELSEEHS"
     gene            complement(1472..>3749)
                     /locus_tag="VFQ35_09385"
     CDS             complement(1472..>3749)
                     /locus_tag="VFQ35_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9930888.1"
                     /translation="DEPQILIALEDLLANRFSVLKTESPEHALNLMAASDDIAVLITD
                     QRMPRMTGDALLSRIEPSRHTLKILLTGYADLSAVIRAVNEGQIFAYVTKPWDPDDLR
                     LKVDKAVEHFQIAQELAYERQLLHDLMDNIPDGIYFKDSELKIRRVNRAFAAALSRAP
                     EELEGRLVDELLGESSLAKEARLEELDVLRRGVPVRDVVREYRTDGVRRFVSETKAPI
                     RLSNGELSGIVGIARDVTERIESEEAIRRGEQRFREQSLMLDSILNSMGDAVIAADSN
                     GRFLVYNREAERLLGPRPADLSAVAWAQNTAILSVDGSVDNPLVGAMLGREIQETELL
                     LKNPEGAQITVSLKATPLTGMGGALLGGIALVRDITQRRELEQQLRHAQKMEGIGQLA
                     GGVAHDFNNLLAVIKSYGELLLREAGDDPERREDLEQLLHAANRGAALTRQLLAFSRR
                     QVVQPRLMQLNDVVTSIDKMLRRVIGEDIDLVTSLSPDLHVIKADSGQIEQVLVNLAV
                     NARDAMPDGGKLTVETANVRLSNGYADSDSSVLEGDYVMVAVSDTGTGMSAETRKRMF
                     EPFFTTKEVGKGTGLGLSTVYGIVQQSGGHIWVYSEPDRGTAFKLYFPRATGTLASEP
                     ARPAPFRRLKNDSTILLVEDDEAVRRVATRILRESGYQVLETGDAEEARRICRERGES
                     LDLLLTDVVMPNVSGPKLAAELKASLPKLKVLYMSGFPGAAVVHSGLLRANARVIEKP
                     FSPAVLVDKVEEAMSVPD"
BASE COUNT          621 a         1213 c         1192 g          723 t
ORIGIN      
        1 ctcgtgcgat gccaagaggc gcgcgttgtc gttgacgtgc ccgacgagtg gcggcacgtc
       61 gatcgcgagc gccggagccg ccgacgccgt gacgaagagc gcgcagaaca gccacaagag
      121 ccgcatcaag atgcggtcga cgacgcgcgc gcgccccgaa agccgcgcca tggttggagt
      181 ccgcgtcaca gattgaccag cgctacaggt tgacgtcggg cgctttttcg agcccgggcg
      241 tgccctcgaa ggtgggccgc gacttcatcc cccgcatgct cgctccgatg ctcgtgggaa
      301 aacgagcgac gaccttgttg tattccgcta cgccgtcgat gtagcgcttg cgcgcgaccg
      361 cgatacgatt ctcggtgccc tcgagctgag cttgcaggtc gcgataggcg tcggttgcct
      421 tgagttcggg atagcgctcg gccacgacca aaaggcgcga caacgcgcca ccgagctccc
      481 cctgtgcttg ctcgaacgct ttgagcttgg ccgggtcgtc gatggtgctc gcgtcgacct
      541 tgatgccagc gactttggag cgcgcttcga ccaccttggt gagcgtgtct tgttcgaatt
      601 gccccgcgcc cttcaccacc ttcaccaggt tcggcacgag atccgcgcgg cgcttgtact
      661 gattctggac ctcggcccag gccgccttca cgtcctcgtc ccgctcgacc acttcgttgt
      721 agccgcagcc cgcggagagc agggcgaaca ctgcgatgaa ggtgccgagc cagagtcgct
      781 tccagaagct cgtcgccttt accaaattcg tatttctcat gaagtgccct cgtgctcgca
      841 gactcgcagc ctgaccattc accgcgattt cgtgaaccgt aacgggctgc gcccgatcca
      901 tatagtaccg gtgcctcgaa aacgcccagg gggggcggtt tatgtcagga gtgttcctcg
      961 gaaagctcga cgtgcacctc ggttttgacc gagttcgcga tgaagcagcc tcggtgggcg
     1021 cgttcgtgga gggcgcggag ctcttccggg gctggttttg aagaaccaaa ccgcacgcgg
     1081 ggcctgagga tgacgcgagt cacggcgagc cgccccccgc cgtccttttc gaggtacccc
     1141 acggcatcgt cgctatactg atccaccagc agtccgcctc gggccgcgag cgccaagaac
     1201 gtcatcatgt gacagctcga gagcgcgctc accaagagct cttcggggtt tacgcgattc
     1261 gcgtctccgc gatactcctc cgtggccgag agcttcaggc tctggccagt cccgaacgag
     1321 acttcgtggt tgcgcgagaa cgaagcgagc gaaaaatcgt ctcccgtgcg ttgccactcg
     1381 atgcgcgtgc ggtgctccga catcggcgca gtttcgcaga aatcgctcgg gggcgctcag
     1441 ttatcgcatc cgaggccgaa ccgtgctcgt ctcagtccgg aaccgacatc gcttcttcga
     1501 ccttgtcgac gaggacggct ggtgagaagg gcttctcgat gacgcgcgca ttggcccgga
     1561 gcaaccccga gtgaacgacg gcagcaccgg ggaagcccga catgtagagc actttgagct
     1621 tgggcaacga cgccttcaat tcggccgcga gcttcggccc actcacgttc ggcatcacca
     1681 cgtcggtgag gagtagatcg aggctctctc cgcgctctcg gcagatgcgg cgcgcctcct
     1741 cggcgtcgcc ggtttcgagt acctggtagc cgctctcgcg caagatgcgg gtcgcgacgc
     1801 gacgcaccgc ctcgtcgtct tcgaccaaga ggatggtcga gtcgttcttg aggcgtcgaa
     1861 acggagcggg ccgcgccggt tcgctcgcga gcgtgccggt cgccctcggg aagtagagct
     1921 tgaacgccgt gcctcgatcc ggctcactgt agacccagat gtgcccgccg ctctgctgga
     1981 cgattccgta gacggtcgag agcccgagcc cagtgccctt cccgacctcc ttcgtggtga
     2041 agaacggctc gaacatgcgc ttgcgtgtct ccgcgctcat gcccgttccg gtgtcgctca
     2101 cggccaccat cacgtagtcg ccctcgagca cgctcgaatc ggagtccgcg tagccattcg
     2161 agagccggac gttcgcggtc tcgacggtga gctttccccc atctggcatc gcgtcgcgcg
     2221 cgttgacggc gaggttcacg agcacctgct cgatttgccc cgagtccgct ttgatcacgt
     2281 ggaggtcggg tgacagagac gtgacgaggt cgatgtcctc tccgatgacg cgccgaagca
     2341 tcttgtcgat gctcgtgacg acgtcgttca gctgcatcaa ccgcggctga accacttggc
     2401 ggcggctgaa cgcgagcagc tggcgcgtga gcgcggcgcc gcgattcgcg gcgtgcagga
     2461 gctgctcgag atcttcgcgc cgctccgggt cgtcaccggc ctctctcagc aagagctcgc
     2521 cgtagctctt gatcacggcc agcaaattgt tgaagtcgtg cgcgacgccg cccgcgagtt
     2581 gcccgatacc ttccatcttt tgggcgtgcc gcagctgctg ctcgagctcg cggcgctgcg
     2641 tgatgtctcg cacgagcgcg atcccaccga gcaaggcgcc gcccattccg gtgagagggg
     2701 tcgccttcag cgatacggtg atttgagcgc cttccgggtt cttcagtaga agttcggtct
     2761 cctgaatctc gcggccgagc atcgctccga cgagcgggtt gtcgacgctc ccgtctacgc
     2821 tgaggattgc ggtgttctgc gcccaggcga ccgcggagag gtctgcgggg cgggggccga
     2881 gaagccgctc cgcctcacgg ttgtaaacga ggaagcgccc gttgctgtcg gcggcgatga
     2941 cggcgtcgcc catgctgttc aaaatcgaat ccagcatcaa cgactgctcg cgaaaccttt
     3001 gctccccgcg gcggatcgcc tcttcgctct cgatccgctc ggtcacgtct cgtgcaatgc
     3061 cgacgatgcc gctgagctct ccgttactga gacggatcgg cgccttggtc tcggagacga
     3121 agcggcgtac gccgtcggtt cgatactcgc ggaccacgtc gcgcaccggg acacccctgc
     3181 gcagtacgtc gagctcttcg aggcgagcct ccttcgcgag cgaactttct cccaagagct
     3241 cgtcgacgag cctgccttcg agctcctcgg gcgcgcggct cagcgccgcg gcaaacgcgc
     3301 ggttcactcg gcgaatcttg agctccgagt ccttgaagta gatgccgtcg gggatgttgt
     3361 ccatgaggtc gtgcaagagc tgccgctcgt aagcgagctc ctgcgcgatc tgaaaatgct
     3421 cgaccgcctt gtcgaccttg agcctgagat cgtctggatc ccaaggcttg gtcacgtacg
     3481 cgaagatctg gccctcgttg acggcgcgga tcacggccga gagatcggcg tagcccgtga
     3541 gcaggatctt caaggtgtgg cgcgatggct cgatgcgcga caagagcgcg tcaccggtca
     3601 tgcgcggcat gcgttgatcc gtgatgagta cggcgatgtc gtcgctcgcc gccatcagat
     3661 tcaaggcgtg ctcgggcgac tccgtcttca aaacggaaaa tcgattggcg agcagatcct
     3721 cgagggcaat gaggatctgc ggctcgtcg
//
LOCUS       DASTKS010000449         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1856814, whole genome shotgun sequence.
ACCESSION   DASTKS010000449 DASTKS010000000
VERSION     DASTKS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856814"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1095
                     /locus_tag="VFQ35_09390"
     CDS             85..1095
                     /locus_tag="VFQ35_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9930889.1"
                     /translation="MAGRAWFLGISLLGLVVTMGCGAATGGHEASGGKTSSSSGGAPF
                     AGAASVGGGTNVGGGTNVGGGTNVGGGANVAGATNTGYVGGRTNVDWTSGGSPGDTCG
                     VDTDHDGIPDEIEGSLSSRDTDHDSVPDYLDDDSDDDGLPDSLEGRFASCEGAPDFDA
                     DSIPDYLDRDSDGDGIGDADEPRYGTDPYESDTDGDGCYDSVNVFLGECSLDSDRVIS
                     SECKSQVAQTRLVLRVRSDFEGSLDDVSIAVRINREFASNNAGVSVTPVDVIPSDAAV
                     IEQGRVHAVRQGALLGLVVQVAVPDPNLVVNVDVVSAERGVLARGSAIVKALVGSCPP
                     PG"
     gene            1201..1791
                     /locus_tag="VFQ35_09395"
     CDS             1201..1791
                     /locus_tag="VFQ35_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930890.1"
                     /translation="MSRKPDTSLSKAAALLEAELAHFEDLLQELKAPVNSEKALQRAR
                     HRLEECSACEEKLAEHLRAFAEAMQVMQARQQECMQLLAERAEGVQARLLDRNALVAR
                     INQLGQQARAVAEPITSIDEPAWSSPTPELLSSVAEMSSRLEAVIEEAAAVAASAKDS
                     DWSDVARDADALRQQLTAMRGKLILGQRKLASRAPS"
     gene            complement(1794..>2386)
                     /locus_tag="VFQ35_09400"
     CDS             complement(1794..>2386)
                     /locus_tag="VFQ35_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930891.1"
                     /translation="LPAELDGEVLRAAREVETGRPRPLATLVPASEPIVPTPFVTHLR
                     VSLTLSQNLKSAPTAANAESHTSVELGSAVFAEGSSGGSEVSLEGERYRVQVRLSTDT
                     PSDPANGYRTETYRGKLEVRVEDEHGRSFRRILPVSGLVELDGPGAIAAMGLEVPMYL
                     SSDEEQNPRLGALEERGSLPRVQILVSSVLTAEEFE"
BASE COUNT          416 a          696 c          840 g          434 t
ORIGIN      
        1 gtgacgcagg gcgtttttca acccttgccg acgcgatcgg gcgagagcgc gtgtcgaaca
       61 gcgcggatcg gctattctag ggcgatggcc gggcgcgcgt ggtttctggg aatttctctg
      121 ctggggctcg tcgtaacgat gggttgcggg gcggctacgg gaggtcacga agcgagtggc
      181 ggtaagacct cttcgagctc cggaggggca ccgtttgccg gcgcggcgag cgtcggaggc
      241 gggacgaacg tcggcggtgg gacgaacgtc ggcggtggga cgaacgtcgg aggtggggcg
      301 aacgtcgcag gcgctacgaa caccggttac gtcggcgggc gcacgaacgt cgattggacg
      361 agtggcggtt ccccaggcga cacgtgcggc gtagatacgg atcatgacgg catccccgac
      421 gagatcgagg gaagcctgtc gtcgcgcgat accgaccacg actcggttcc agattacctc
      481 gatgacgatt cggacgacga tgggttgccc gattcgctcg aaggccgctt cgcgtcgtgc
      541 gagggggcgc ccgacttcga cgcagattcg attccggact acctcgaccg ggacagcgat
      601 ggagacggta tcggagacgc cgacgagccg cgttacggta ccgatcccta cgaatcagat
      661 accgatggcg atggctgcta cgattcggtc aacgtgttcc tcggtgagtg ttcgctcgac
      721 agtgatcggg tgatctcctc ggaatgcaag tcgcaagtcg cgcaaacgcg cctcgttttg
      781 cgcgttcgca gcgacttcga aggttcgctc gacgacgttt cgatcgcagt ccgaatcaat
      841 cgcgagtttg cttcgaacaa cgccggcgtg agcgttacgc cggtggacgt gataccgagc
      901 gacgcggctg taatcgagca agggcgagtg catgcggtga ggcaaggcgc cttgcttggc
      961 ctcgtggttc aagtggcggt tccggatccg aacctggtcg tgaacgtcga tgtggtgagc
     1021 gccgagcgcg gcgttttggc acgcggctcg gcgatcgtga aggcgctggt gggtagttgt
     1081 cccccgcccg gttgagcgac gcgaaaaaag tgaaggcgac agatcgccgc cggccgtccc
     1141 agtggtcgcc gctttgaagg aacgattgtt acttcaaggc cagagcacta gcatgcgccg
     1201 atgtcgagaa agcccgatac ctcgttgtcc aaggcggcgg ctctgctcga ggccgagctc
     1261 gcgcacttcg aagacttgct gcaagagctg aaagcgccgg tgaactccga gaaggcgctg
     1321 cagcgcgcgc gccatcgcct cgaagagtgc tctgcgtgcg aggagaagct cgcagagcat
     1381 ttgagggctt tcgccgaggc gatgcaggta atgcaggcgc gacagcagga gtgcatgcag
     1441 ctcctggccg aacgagcgga gggagtgcag gctcgcttgt tggatcggaa cgctctcgtg
     1501 gcgcggatca atcagctcgg gcaacaagcg cgagcggtag cggagccgat aacttccatc
     1561 gacgagccgg cgtggtcttc gccgacgccg gagctcttga gttcggtggc agaaatgtct
     1621 tcgcgcctcg aggccgtgat tgaagaggcc gcggccgtcg ccgcttcggc aaaagactcg
     1681 gactggagtg acgtcgcgcg cgacgcggac gcgctgcggc agcagcttac ggcgatgcgt
     1741 ggcaagctca tcttgggcca gcgaaagctc gcgagccggg cgccgtcttg accctactcg
     1801 aactcttcgg ccgtgagcac gctgctgact aggatttgga cgcgcggcag ggagccacgt
     1861 tcctcgagcg cgccgaggcg ggggttctgt tcctcatcgc tcgataggta cataggaacc
     1921 tcgagtccca tcgccgcgat cgcccccgga ccgtcgagct ccacgagccc gctcaccggg
     1981 agaatgcgcc gaaaagagcg gccgtgttcg tcctcgacgc ggacctcgag cttccctcgg
     2041 tacgtttctg tacgatagcc gttcgctgga tcgcttggag tatcggtgct gagcctgacc
     2101 tgaactcgat agcgctcgcc ttcgagcgag acttcgcttc caccgctcga accttcagcg
     2161 aaaacggcgc ttcccaattc gaccgacgtg tggctctcgg cgttcgcggc cgtgggtgcg
     2221 ctcttcaggt tctggctcag cgtcaacgat acccgtaggt gcgtgacgaa gggggtcggc
     2281 acgatgggct cgcttgcggg tacgagcgtc gcgagcgggc gcggccttcc ggtttcgacc
     2341 tcgcgcgcgg cgcggaggac ctccccgtcg agttcggcgg ggagct
//
LOCUS       DASTKS010000450         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_518042, whole genome shotgun sequence.
ACCESSION   DASTKS010000450 DASTKS010000000
VERSION     DASTKS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518042"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFQ35_09405"
     CDS             <1..522
                     /locus_tag="VFQ35_09405"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET9930892.1"
                     /translation="VEFTSKRPDAALKIMRELARRLRQTNAAFSSQVSRNVLQEEAEQ
                     LTLGQRVADRVATFGGSWPFIGTFAALMAAWMSYNALSGKSFDPYPFILLNLMLSTLA
                     ALQAPVIMMSQNRQAVKDKLLAENDYQVNLKSELGIQALLKGQAELIARLGLLERQVT
                     PSRRPTALSTESK"
     gene            complement(554..1594)
                     /locus_tag="VFQ35_09410"
     CDS             complement(554..1594)
                     /locus_tag="VFQ35_09410"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET9930893.1"
                     /translation="MARPPKPGIRCATLAVIQVTEDPRQRAHRLLQRWGWNATSFQTL
                     SEGFSYWFTHEGCVAYAACGGAWVGAGAPIADSDRLSQVANGFVEAAAARGKRASFFA
                     VEQRFLRATKMSSFLVGEQPVYDPGVWCTHLRRSASLREQLRRARAKGVHVSPGVPVD
                     DANELARVHEAWLSRHALPGMGFLTGLDPFSEERPRRYFVARSRENRIEGFAALSPVY
                     ARQGWLFEHLVRSPSAPNGTVELLVDAAMRALADDGVAWATLGLAPLAGDVALPLKWV
                     RRWAEPLYNFEGLRAFKAKLGPVRWDPVYLAYPRTNNGLTALCDGLAAFTRGRPLGFA
                     TRSLSKSLMRSS"
     gene            1659..2780
                     /locus_tag="VFQ35_09415"
     CDS             1659..2780
                     /locus_tag="VFQ35_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9930894.1"
                     /translation="MNTSPRSTTLGSLVFLFACSSNAVDGASSTGGAPSASGGQVSAS
                     GGGSGGLVNASGGTPVIEVPVGGGAAGELNCGFQRFEPKRVPADVVLVLDRSGSMKEE
                     PSGSDSGMSKWEIVVPAVAEVVESTASVVNWGLKGFPEGEGSECVAGSVTDAIDVPVA
                     DDSAPHVVAQMQSIRPEGNGTPTGDAIRAAVTYAKSVANGHKKYLLLATDGEPSCAGT
                     SKDTSKARADATQAVADALSAGLPTYVVGVATTKDSATKALNAMANAGGVAVTGATTQ
                     YYLASSRAALVAALQTIAGDVSKTCVFNLEPAPPAPDFIAVKVSGSIVPRDATSTNGW
                     EYTNAAHTALEIFGAACDTIRQTSEAVEIVYGCQGVVPK"
     gene            3011..3481
                     /locus_tag="VFQ35_09420"
     CDS             3011..3481
                     /locus_tag="VFQ35_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930895.1"
                     /translation="MIHSNSKVVQDAEGLVTWAWLSPGVLYARYEQTLSADVSAAVVA
                     ALTDQVARVESFELYVDASKLVAYELKARSSFVSFVREHRKRCTALALLPWKAEAPFI
                     TPAALDSLGQPTEIVADANDFGLRLLRVVPAAPELVASLRNLASEERRSFSVLR"
     gene            3713..>4040
                     /locus_tag="VFQ35_09425"
     CDS             3713..>4040
                     /locus_tag="VFQ35_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930896.1"
                     /translation="MRTSGVWVLCALFGVACSTAPDDQGGAGGVNNAAGGNSDGSGGS
                     PSGGNTTAQGGAQSGGSANGGSDSNAQKGGASGSSAGGASSGGSAGGGRGGSGGRGSA
                     SGGRAGS"
BASE COUNT          718 a         1254 c         1401 g          667 t
ORIGIN      
        1 gtcgaattca cgagcaagcg cccggacgcc gctctcaaga tcatgcgaga gctcgcccgg
       61 cgcttgcgcc agacgaacgc cgcgttttcg agccaagtct cgcgcaacgt tttgcaagaa
      121 gaagccgagc agctgacgct cggtcagcgc gtcgcggatc gcgtcgcgac gttcggcggc
      181 tcttggccat tcattgggac gttcgcggcg ctgatggcgg cgtggatgtc gtacaacgcc
      241 ttgtcgggca agtcgttcga cccgtacccc ttcatcttgt tgaacttgat gctctcgacg
      301 ctcgctgccc tgcaagctcc cgtgatcatg atgagccaaa accggcaggc agtgaaagac
      361 aagctcctcg ccgagaacga ctatcaggtg aatttgaaga gcgagctcgg cattcaagcg
      421 ctgctcaagg gccaagccga gttgattgcg cgcctgggat tgctcgagcg gcaggtgaca
      481 ccgtctcgcc gcccgaccgc gctttcgacc gagtcgaagt gagcggacgc gacctctcgc
      541 gaacctgctc ggatcaagac gagcgcatca ggctcttcga aaggctgcgc gtcgcgaagc
      601 cgagcgggcg gccgcgcgtg aacgcggcga gcccgtcgca gagggcggtg agcccgttgt
      661 tcgtgcgcgg ataggcgaga tatacggggt cccagcgaac gggaccgagc ttcgctttga
      721 aggcgcgaag gccctcgaaa ttgtagagcg gctccgccca acggcggacc cacttgagcg
      781 gtagcgcgac gtctccggcg aggggagcca gcccgagcgt cgcccacgcg accccatcgt
      841 cggcgagagc gcgcatggcg gcatcgacca agagctctac cgtcccgttc ggcgcgctgg
      901 gcgagcgcac gagatgctcg aagagccagc cttgtcgcgc gtaaacaggg ctcaacgcgg
      961 cgaagccctc gattcggttt tcgcgcgaac gagcgacgaa gtagcgccgc gggcgctctt
     1021 cgctgaatgg gtcgagcccc gtgagaaagc ccatgccggg cagcgcatgg cgagagagcc
     1081 aggcctcgtg aacgcgcgca agctcgttgg cgtcgtcgac ggggacgccc ggcgagacgt
     1141 gcacgccctt ggctcgcgcc cggcgaagct gttcgcggag gctcgccgag cggcgcaggt
     1201 gcgtgcacca gacccctgga tcgtaaacgg gctgttcacc cacgaggaac gaagacatct
     1261 tcgtcgctcg tagaaagcgt tgctcgaccg cgaagaagct cgcgcgtttc ccgcgcgcgg
     1321 cagcggcctc gacgaagccg ttcgcgacct gggagaggcg gtccgaatcc gcgatgggtg
     1381 ccccggcgcc gacccaagcg ccgccgcacg cggcgtaggc gacgcagccc tcgtgggtga
     1441 accagtacga aaagccctcg ctcagggtct gaaaggacgt tgcgttccaa ccccagcgtt
     1501 gcaagagtcg gtgggctcgt tggcgggggt cttcggtgac ttggatcacg gcgagcgtcg
     1561 cgcaccttat accgggtttg ggcgggcgcg ccatgtggcg aggacgtcct tgggccgtcg
     1621 cgcgagcctc gcgccacttg ttcctctcga ggagccgaat gaacacttcc ccgcgttcca
     1681 caacgctcgg atcgttggtt tttctgttcg cttgttctag caacgccgtc gacggcgcaa
     1741 gctcgaccgg aggcgctccg agcgcgtcgg ggggccaggt cagcgcgagt ggtggcggaa
     1801 gcggcggtct cgtcaacgcg agcggcggaa cgcccgtgat cgaagtgccg gtcggcggtg
     1861 gcgcggcggg cgagctcaac tgcggcttcc aacgcttcga gccgaaacgc gtcccggcgg
     1921 atgtagtcct cgtgctggat cgctcgggct cgatgaagga agagccgtcg ggctccgact
     1981 ccggaatgtc gaagtgggaa atcgtagtgc cggcggtggc cgaagtcgtc gagagcaccg
     2041 cgagcgtcgt gaactggggt ctcaagggct ttcccgaggg ggaaggcagc gagtgcgtgg
     2101 ccggctctgt cacggatgcg atcgacgtgc ccgtcgcaga cgacagcgcc ccgcacgtgg
     2161 tcgcgcagat gcagtcgatc cgacccgagg gcaacggcac gccaaccggc gacgccattc
     2221 gcgcggccgt gacctacgca aagagcgtgg cgaatgggca caagaagtac ctcctgctcg
     2281 cgaccgacgg cgaaccatcg tgcgctggga ccagcaaaga cacctcgaag gctcgcgccg
     2341 acgcgaccca agcggtcgcc gacgcgctga gcgcagggct tccgacgtac gtggttggag
     2401 tcgcgacgac caaagacagc gcgaccaaag cgctcaacgc catggcaaac gccggtgggg
     2461 tcgcggtcac gggagcaacg acgcagtact acttggcgag cagtcgcgcc gcgctcgtgg
     2521 cggctctgca aaccatcgcc ggggacgtgt cgaagacctg tgtcttcaac ctggaaccgg
     2581 ctcccccagc gccggacttc atcgccgtga aggtgtccgg atcgatcgtg cctcgcgatg
     2641 ccacctccac gaacggctgg gagtacacga acgctgccca cacggcgctc gaaatattcg
     2701 gcgccgcttg tgacacgatc cggcaaacat cggaggcggt cgagatcgtc tatggctgcc
     2761 agggagtcgt gccgaaatag gcccgaaaag tgtggcgaac gcgttgagtc actgatttcg
     2821 gtcagatagg cgcttcgtcg aagcggcacg aattccgccg aattatcgga aaatgcgctt
     2881 cgcgacgagc gcgcagcgca gcgctcactt atcgtacctg ccccctgtcc gtagctcacg
     2941 cagcctcgtt gctgcaaagg caatcgggtt tcggtaagag tgcggcgtag gtcgcacttg
     3001 tggcagtacc atgatccatt cgaactcgaa ggtggtccaa gacgccgagg gcttggtcac
     3061 ctgggcctgg ctctctccgg gcgtgctcta cgcccgttac gaacaaaccc tatccgccga
     3121 cgtcagcgcg gcggtcgtag cggccctgac cgaccaggtc gcgcgggtcg agtcgttcga
     3181 gctctacgtc gacgcgagta agctagtcgc gtacgaactc aaagcgcgca gctcgttcgt
     3241 cagcttcgtg cgtgagcacc gaaagcgatg cacggcgctc gccttgctcc cgtggaaggc
     3301 ggaggctccg ttcatcacgc ctgccgctct cgattcgctg ggacagccta cggagatcgt
     3361 cgctgacgcg aacgatttcg ggctgaggct cttgcgggtc gtgccggcgg cgccagagct
     3421 cgttgcctcg ctgcgaaacc tcgcgagcga agagcggcgc agcttcagcg tgctccgcta
     3481 gcctcgtccg gccgctaggt gacggtgttg tcgacgacct gagcgccgcc tcgggcgcgc
     3541 ttcctcgctc aagttttctt ggctctcgcg acgggcagga acctgggaca ctccctagtc
     3601 ctgagtgggt atgctgcata cccgactcat attggaaagt gacgctctca gcggttctgc
     3661 atcgcgcgtc gaatgttgag ccgcggcatt gggtactgag tcactgatgg taatgcgaac
     3721 atcgggcgtc tgggttttgt gtgccctttt cggcgtcgct tgcagcaccg cgcccgacga
     3781 tcagggtggc gctggcggag tgaacaacgc ggccggggga aacagcgatg gctcgggtgg
     3841 cagtccctcg gggggaaaca cgacagcgca gggtggggcc cagtcgggag gaagcgcgaa
     3901 tggtgggtcc gattcgaatg cgcagaaagg tggggcttcg ggctccagcg cgggtggcgc
     3961 gtcgagcggc ggaagcgcgg gcggaggacg aggcggcagt ggagggcgcg gcagtgcgag
     4021 tgggggtcgc gccggaagcg
//
LOCUS       DASTKS010000451         5555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_740097, whole genome shotgun sequence.
ACCESSION   DASTKS010000451 DASTKS010000000
VERSION     DASTKS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740097"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5555
                     /locus_tag="VFQ35_09430"
     CDS             <1..>5555
                     /locus_tag="VFQ35_09430"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET9930897.1"
                     /translation="ARPALQRLEREERAPSDAFLVQLLRRLRAHDAPSVEVTDWIRRH
                     CERKGTTPEELARREHLRQAADQVSVGNSITSMRQIAALDWSKFFEQVSTVEALLGED
                     PSGAHRASDDASRDRCRHAVEKLARRSSKSEAEVARLALDMARAAEARGADVAARHVG
                     YYLVDRGRKELERRIGYRFSAAEWVTRPLLAAPSFFYLGTILLVTALLAVAVFRISTQ
                     AGAATGIVWLVVVLALVPASEIAVSFANALVIALLPPRLLPKLQLEDGISPEHTTLVV
                     VPSLLDSANTLATLLEDLEVRSLANPEKNLYFALLTDFTDAPTRDRDGDAELLQLAEA
                     GIRALNARHSRPDAHRFWLLHRERRQNPAEGKFMGWERKRGKLEELNHLLRGGSDTSF
                     SVVSGPRELLHKVRYVITLDADTELPRDVARKLVGTLAHPLNQAVIDKVRRRVVSGHG
                     ILQPRVGTLPLSSRRSRFARIFAGPPGIDPYTSAVSDVYQDLLGEGSFVGKGIYDVDA
                     FLEVMSGRVPENTLLSHDLFEGNLLRSALVTDIEVLDEQPDAYAVVASRQHRWMRGDW
                     QLLPWLLPRVPTAKGSAPNPFRAMDLWKFVDNLRRSLMPPTLLALALVSWLTGGTTAV
                     LGSLAIALVFLSPVLCRALLDALRGGNRSQSRLSALGGDLKANSQQLVLNVSFLVDQA
                     LLSLDAIGRTLYRLLVSRRLLLEWTTMSQASQRFRRGGSVEPRMWLAFGLAVAALVAL
                     LAVAPGSVPLALPAVVLWGGAPFLALWLRMPEPVRDKTAELTAADRRLLRAVARKTWR
                     FFDEFVGDEDNHLPPDNYQEDPRGVVAHRTSPTNIGLYLLSVVAARDFGYVSLRELVL
                     RLEKTLGTIERLEKRNGHILNWYETTTLRPLEPQYVSTVDSGNLAGYLWTLREAVGEL
                     ATAPFVSRNVFEAARDALHLASQQLARGKNKDAELARRIASADRRLEEAVERVGTEPL
                     ADHALLLELASVTALLASDESRALDEEARYWVEQASATLFQCGEEIASLAPFLSRLKA
                     APAALASGELASEWAELVTTLGRALSLDGLTNLGADARERVVGLRQRLEASTLSEKAE
                     AADFLSEFERLLDSSRERGSRLKEQLSAVGTRAGALGDGMDFSFLFDEERSLFSIGYN
                     VGSMRLDGSHYDLLASEARLASLLSVAKGDAPQQHWFRAGRPRTVVDSRGVLLSWSGS
                     MFEYLMPLLVTYGYSDTLLDETYSTCVYAQRKYGNEKGVPWGISEAAYNVMDLSMTYQ
                     YRAFGVPGLGLKAGLGEDLVIAPYATALAAMVEPKLATQNMRALAKEGLDGAYGFYES
                     IDYTPAHLPPGRRGVVVKAYMAHHQGMTLVALDNVLFGMPMARRFHNEPRIKASALLL
                     EERLPTTAPVVEVRATQIAAPAPSEPEVYTAEHVGLGGNEGARVHLLGHGELSTLLTA
                     TGGGFTTWKNLDVTRFREDASLDPGGIYIYVRDHKRNAMWSAAHLPTQRTPDFYDAAF
                     HIDRVAFHRRDGSIETLTEIVVSPENPAEVRRITLTNHGETEAEIDITTYSEIVIGQR
                     AADLAHPAFSSMFIETEAIPARGALLARRRPRSPSEAPVWVVQVLSPGNDEFSELSFD
                     TSRPNFIGRGGTLAEPLAMRFGHELSGTLGCVLDPAFTLRRSVRLAPGARASLTLTTA
                     LASSRETALELVEIHATPHHAERTFGLAFADARVELKHLGITATQSHRFQRLLSSVLF
                     PQPALRATPDPTSLRGKGRSALWAQGISGDLPIVVLRIDDADFHELCRELLLAHEFFR
                     LNGVSVDLVLLNEEPSGYLLPMQDAVLDLVRSSPAQAHLDQRGGVFVRRTALLNEDDR
                     TLLMCAARV"
BASE COUNT          864 a         1884 c         1829 g          978 t
ORIGIN      
        1 cgcgcgccca gcgctccagc gcctcgaacg ggaagagcgg gcgccgagcg acgcattttt
       61 ggtgcagctc ttgaggcggc tccgcgccca cgacgccccg tcggtcgagg tcaccgactg
      121 gattcgccgg cattgcgagc ggaagggaac cacccccgaa gagctcgcgc gccgggagca
      181 ccttcgtcag gccgcggatc aggtttcggt cggaaactcg atcacgagca tgcggcaaat
      241 cgccgcgctc gattggagca agttcttcga gcaggtgagc acggtcgagg ctctgctcgg
      301 cgaagatccg agtggcgccc accgcgcgag cgacgacgcg agccgcgacc gctgtcgcca
      361 cgcggtcgag aagcttgctc gccggagctc gaagagcgag gcagaagtgg cccgcctggc
      421 cctcgatatg gcgcgggcgg ccgaggctcg cggagccgac gtcgccgcga gacacgtcgg
      481 ctattacctg gtcgaccggg gccgaaagga gctagagcgc cgcatcggct accgcttcag
      541 cgccgcggag tgggtgactc gcccgctcct cgccgcgccg agcttctttt atctcggaac
      601 gattctactc gttaccgcgt tgctcgcggt ggcggtcttc cgcatctcga ctcaagccgg
      661 agccgcgacc gggatcgttt ggctagtggt cgtgctcgcg ctcgtccccg ccagcgaaat
      721 tgcggtgtct ttcgcaaacg cgctcgtcat cgcgctgctg cctccgcggc ttctgccgaa
      781 gctccagctc gaagacggca tttcgccgga gcacacgacg ctcgtggtcg tgccgtcgct
      841 cttggacagc gcgaacactt tggcgactct cctcgaggat ctcgaggtgc gttcgctcgc
      901 gaacccggag aaaaacctat acttcgcgct gctcaccgac ttcaccgacg cgcccacgcg
      961 agaccgcgac ggcgacgccg agctcttgca gctcgccgag gcggggatcc gtgcgctcaa
     1021 cgcgcgtcac tcgaggcccg acgcgcatcg cttctggctc ttgcaccgcg agcgtcgtca
     1081 gaaccccgcc gagggcaagt tcatgggttg ggagcgcaag cgtggcaagc tcgaagagct
     1141 caaccacctg ctgcgcgggg ggagcgacac gtcgttctcc gtggtgagcg gccctcgcga
     1201 gctcctgcac aaagtccgct acgtcatcac gctcgacgcg gacacggagc ttcctcgcga
     1261 tgtagcgcgc aagctggtcg gtacgctggc gcatccgctg aaccaagccg tcatcgacaa
     1321 ggttcgccgg cgcgtcgtga gtgggcacgg gatcttgcag ccacgcgtgg ggacgctccc
     1381 gctgagctcg cggcgctcgc gcttcgcgcg catcttcgcg ggtccccccg ggatcgaccc
     1441 gtacacgagc gccgtgtcgg atgtgtatca agacctcctt ggagaaggct cgttcgtcgg
     1501 taaaggcatc tacgacgtcg acgcatttct cgaggtgatg tcgggtcgcg tgccggagaa
     1561 cacgctgctc agccatgact tgttcgaggg caatctcctg cgctcggcgc tcgtgacgga
     1621 tatcgaggtt ctcgacgagc agcccgacgc atacgccgtc gtcgcgtcgc gccagcaccg
     1681 ctggatgcgc ggggattggc agctcttgcc ttggcttttg ccgcgggtgc cgaccgcgaa
     1741 gggtagcgcg cccaacccgt ttcgcgcgat ggacttgtgg aagttcgtcg acaacctgcg
     1801 ccgcagcctg atgcctccga ccttgctcgc gctcgcgctc gtgtcgtggc tcacgggtgg
     1861 gacgacggcg gtcttgggct ctctcgccat cgcgctcgtg ttcctctccc cggtcctgtg
     1921 ccgcgccttg ctcgatgcgc tgcggggcgg caaccgctcg cagtcgcgct tgagcgcgct
     1981 cggcggcgac ctcaaagcga actcgcagca gctcgtcctg aacgtctcgt tcctcgtcga
     2041 tcaggcgctg ctttcgctcg acgcgattgg gcgaactctc tatcggttgc tcgtttcacg
     2101 ccggctcttg ctcgaatgga cgacgatgag ccaggcgagc cagcgcttcc gacgcggggg
     2161 cagcgtcgag ccgcgcatgt ggctggcgtt tgggctcgcg gtcgcggcgt tggtcgcgct
     2221 tctcgcggtc gcgccgggtt cggttccgct cgcgctcccc gccgtcgtac tctggggcgg
     2281 cgcgccgttc ctcgcgctgt ggctcaggat gcccgagccg gttcgcgaca agaccgccga
     2341 gcttaccgcc gccgatcgac gtctgctgcg cgcggtggcg cgcaagacgt ggcgcttctt
     2401 cgacgagttc gtgggcgacg aagacaatca cttgcctccc gacaactatc aggaggatcc
     2461 gcgcggcgtc gtcgcgcatc gcacgtcgcc gacgaacatc gggttatatc tgctgtccgt
     2521 cgtcgcggcg cgcgactttg gctacgtgtc gctccgcgag ctcgttctgc gcctcgaaaa
     2581 gacgctcggt acgatcgagc gactcgagaa gcgcaacggc cacatcctca attggtacga
     2641 gacgaccacg cttcgtccgc tcgagccaca atacgtttcg accgtcgata gcggcaacct
     2701 cgccggctac ctgtggacgc tgcgggaggc ggtcggtgag ctcgcgacgg ccccgttcgt
     2761 cagccggaac gtgttcgagg cggcgcgcga cgcgcttcat ctcgcgtctc agcagcttgc
     2821 tcgcggcaag aacaaggacg ccgaactcgc gcgtcgcatc gcgagcgccg atcggcgcct
     2881 cgaggaggcg gtcgaacggg tcggaacgga gccgctcgcc gatcacgccc tgctgctcga
     2941 gctcgcctcg gtcacggccc tgctcgcttc cgacgagtcg cgggctctcg acgaagaggc
     3001 tcgctactgg gtcgaacagg cgagcgccac gctgtttcag tgcggcgaag agattgcgtc
     3061 gctcgcgccg ttcctgagcc gtttgaaggc ggcaccggcc gcgctcgcgt cgggtgagct
     3121 tgcgagtgag tgggcagagc tcgttacgac gctgggccgc gcgttgtctc tcgacggact
     3181 cacgaacctg ggcgccgacg cacgcgagcg ggtcgtcggg cttcgccagc ggctcgaagc
     3241 gtcgaccctc tccgaaaagg cggaagcagc cgacttcttg agcgagttcg agaggctgct
     3301 cgattcgtcg cgcgaacgcg gcagccgctt gaaagaacag ttgtcggcgg tggggacgcg
     3361 cgcgggagcg ctcggagacg ggatggactt cagcttcttg ttcgacgagg agcgcagcct
     3421 gttctcgatt ggatacaacg tcggcagcat gcgcctcgac gggtctcact acgatctgct
     3481 cgcgtcggag gcgcgcctcg cgagcttgct ctccgttgcg aagggggacg ctccgcagca
     3541 gcactggttc cgcgctgggc ggccccggac ggtcgtcgac tcgcgcggcg tgctgctctc
     3601 gtggagcggc tcgatgttcg agtacctcat gccgctgctc gtgacttacg gctactcgga
     3661 cacgctgctc gacgagactt actcgacctg cgtctacgcc caacgcaagt acggtaacga
     3721 gaagggagtg ccctggggca tctccgaggc cgcctacaac gtcatggacc tcagcatgac
     3781 gtaccaatac cgcgccttcg gcgtgccggg gctcgggctc aaggcgggcc tcggcgaaga
     3841 tctcgtgatc gctccgtacg caaccgcgct cgcggcgatg gtcgaaccca agctcgcaac
     3901 gcaaaacatg cgggcgctcg cgaaggaggg gcttgacggc gcttacggct tctacgagtc
     3961 catcgactac acgccggcgc acttgccgcc gggacgccgc ggcgtcgtcg tgaaggcgta
     4021 catggcgcat caccagggca tgacgctggt ggcgctcgac aacgtgctct tcggcatgcc
     4081 catggcgcgg cgcttccaca acgagccgcg catcaaggcg agcgcgctgc tgctcgaaga
     4141 gcgcctgccg accacggctc cggtcgtcga ggtgcgcgca acgcagatcg cggcgccggc
     4201 accctcggag ccagaggtct acacggccga gcacgttggg ctcggcggga acgaaggcgc
     4261 gcgcgtgcac ctgctcggac acggagagct ctcgaccctg ctcaccgcga ccggcggcgg
     4321 cttcacgacc tggaagaacc tcgacgtcac tcgctttcgc gaagacgcct cgctggatcc
     4381 gggtggcatc tatatctacg tgcgcgacca caagcgaaac gcgatgtggt ccgccgctca
     4441 cctccccacg cagcggactc ccgacttcta cgacgccgcg ttccacatcg atcgcgtcgc
     4501 cttccaccgc cgcgacggct ccatcgagac gctgaccgaa atcgtggtgt cgccggagaa
     4561 tccggccgag gtgcggcgta tcacgctcac gaatcacggc gagaccgaag ccgaaatcga
     4621 catcacgacg tactcggaaa tcgtcattgg ccaacgcgcg gcagacctcg cgcaccccgc
     4681 gttcagcagc atgttcatcg agaccgaggc gattccggct cggggagcgc tgctcgcgcg
     4741 acgacgtccg cgctctccgt ccgaggcgcc agtgtgggtc gttcaggtgc tttcgcccgg
     4801 aaacgacgaa ttcagcgagc tcagcttcga cacttcgcgt ccgaatttca tcggtcgcgg
     4861 tggaacgttg gccgagcctc tcgcgatgcg cttcggtcac gagctcagcg gcaccctggg
     4921 ctgcgtgttg gatccggcct ttacgttgcg tcgcagcgtt cgcttggctc ccggcgctcg
     4981 agcgagcctc accctgacga ccgccttggc ttcatcgcgg gagacggcgc tcgagctcgt
     5041 ggagatccac gccacgcccc accacgccga acgcaccttt gggctcgcgt tcgcagacgc
     5101 gcgcgtggag ctgaagcacc tcggtatcac ggcaacgcag tcgcatcgct tccagcggct
     5161 gctctcgtcg gtgttgtttc cgcagcccgc tctgcgcgcg acgcccgacc cgacctccct
     5221 gcgcggaaag ggtcgtagcg cgctttgggc acaagggatc tccggggacc ttccgattgt
     5281 tgtgctgcgc atcgacgacg cagacttcca cgagctttgt cgcgagctcc tgctcgccca
     5341 cgagttcttc cgattgaatg gggtgagcgt cgatctcgtg ctcctgaacg aggagccgag
     5401 cggatatctg ctgccgatgc aagacgccgt gctcgacctc gttcgatcga gccccgcgca
     5461 agctcacctc gaccagcgcg ggggcgtttt cgtgcgaagg accgcgctct tgaacgagga
     5521 cgatcgcacc ttgctcatgt gcgcggcgcg cgtcg
//
LOCUS       DASTKS010000452         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1632981, whole genome shotgun sequence.
ACCESSION   DASTKS010000452 DASTKS010000000
VERSION     DASTKS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632981"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1520)
                     /gene="istA"
                     /locus_tag="VFQ35_09435"
     CDS             complement(30..1520)
                     /gene="istA"
                     /locus_tag="VFQ35_09435"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9930898.1"
                     /translation="MIPKETEAEVLRLFHAEKWKIGTIARQLSVHHTTVQRVLRQAGV
                     EMKAVAPRPSMADSFVPFIVEQLEKYPSLCASRLFEMVKERGYAGGGDHFRRVVARHR
                     PRRPAEAFQRLKTLPGEQAQVDWAHFGKLIVGRAERPLWAFVMVLSFSRQVFLRFLLG
                     ASMPFFVRGHVEAFAFFAGVPRVLLYDNLKSAVLERRGDAIRFNPRLLELAAHYRFEA
                     RPVAVARGNEKGRVERAIRYVRDSFFTARKYTDLADLNRQADDWARGAASDRKWVADK
                     SRTVRDAFAEERPRLLQAPHEPFPVHERVEVEVGKTPYVRFDLNDYSVPHDRTRRSLT
                     VIADLDTVRVIDGSDEVIATHRRCWSRGEQIEAPEHIQRLVEEKKRSREHRGLDRLAK
                     AVPSSQAFLRCLAERGQNLGSNTSRLLLLLDAEGADALEDALVEVLERDTIHIGAVRQ
                     VLDRRRSERGVAPPVAVALAPSEHRDLVVVPHALSTYDSLKKEESP"
     gene            complement(1751..1945)
                     /locus_tag="VFQ35_09440"
     CDS             complement(1751..1945)
                     /locus_tag="VFQ35_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930899.1"
                     /translation="MSSSGCARAAIEGAGTVCRARRRSDAILFDGRVLLTADDHARGG
                     SNLIAKLDIAIGWPSSRRKK"
     gene            2038..2169
                     /locus_tag="VFQ35_09445"
     CDS             2038..2169
                     /locus_tag="VFQ35_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930900.1"
                     /translation="MQPSLAPETQKARELPLSGFRYFSGRAFYPIALGPAQIRAGER"
BASE COUNT          473 a          810 c          841 g          439 t
ORIGIN      
        1 cggcaatacg tcgtcggcac ttaaagtggt catggtgatt cttccttttt gagtgagtcg
       61 tacgtggaga gcgcatgggg aacgacgacg aggtcgcggt gctcgctggg tgcgagcgcg
      121 acggcgacgg gcggcgcgac accgcgctcg gagcggcgtc ggtcgagcac ctgacgcacg
      181 gcgccgatgt gaatcgtgtc gcgctcgagc acttcgacga gtgcgtcttc gagcgcgtcg
      241 gctccttctg cgtccaggag taggagtaaa cgcgacgtgt tgctgccgag attctggccg
      301 cgttccgcga ggcagcggag gaacgcctgg ctgctgggga ccgctttggc gagacggtcg
      361 agcccgcgat gctcgcggga gcgtttcttt tcttcgacca aacgctggat atgctcgggt
      421 gcctcaatct gctcgccgcg gctccagcag cggcgatgcg tggcaatcac ctcgtcgctg
      481 ccgtcgatga cgcgaacggt gtcgaggtcg gcgatgaccg tcaggctccg ccgtgtccga
      541 tcgtgcggca cagaatagtc gttgagatcg aagcggacgt aaggtgtctt cccaacttcg
      601 acctcgacgc gctcgtgaac gggaaacggc tcgtggggcg cctgcaacag tcgcggacgc
      661 tcctctgcaa aagcatcacg aacggtgcgc gatttatctg cgacccattt gcggtccgac
      721 gccgcgccgc gcgcccagtc gtcggcttgg cgatttagat cggcgaggtc cgtgtacttg
      781 cgagcggtaa agaacgaatc gcgcacgtac cggatggcgc gctcgacgcg acccttctcg
      841 ttgccgcgag ccacggcgac tgggcgagcc tcgaagcgat agtgggctgc gagctcgagg
      901 agcctaggat tgaagcgaat cgcgtcaccg cgacgctcca ggacagcgct cttgaggtta
      961 tcgtagagca gcacgcgcgg aacgccggcg aagaacgcga aagcctcgac atgacctcga
     1021 acaaagaagg gcatcgacgc tccgagcaag aagcggagaa acacttgccg cgagaaactc
     1081 agaaccatga cgaatgccca cagcggtcgc tcggcgcgac cgacgatgag ctttccgaag
     1141 tgcgcccaat cgacctgcgc ttgctcgccc ggtagcgttt tcagccgctg aaacgcctcc
     1201 gcaggtcttc tcggacgatg acgcgcgacg acgcgacgga agtgatcgcc gccgcccgcg
     1261 tacccgcgct ccttgaccat ctcgaaaagc cgactcgcac aaagactcgg gtacttttcg
     1321 agctgctcga cgatgaacgg aacgaacgaa tccgccatcg acgggcgtgg cgcaacagcc
     1381 ttcatctcga cgccagcctg tcgaagtacg cgctgcacgg tcgtgtggtg aacactgagc
     1441 tgcctggcga tcgtgccgat tttccacttc tcggcgtgga agaggcgaag cacctccgct
     1501 tctgtttcct tcgggatcac gggcgcctcc gctcactgcg gcgggccgcg aggcgtccac
     1561 ggcgctcgtc gaacgctcgc gcgcggtcgt ggcgctgact gccaggccca tgtgggcaac
     1621 acggctcgac aaaacgagca gcgctgcgga tcgaaaacca acgcatcatc tcggtccttt
     1681 ctgccgaaga cgggctccgg cacgaccggt tccctcgccg gtgctgcggc ttgcgtcacc
     1741 ggctgatgcg tcactttttg cgccgcgaac ttggccagcc gatcgcgata tcgagcttgg
     1801 cgatcagatt ggagccaccg cgcgcgtggt cgtcggcggt aagaagcacc cgcccgtcga
     1861 acagaatcgc gtcgctgcgc cttcgagcac ggcagacagt accggcgccc tcgatcgcag
     1921 cgcgcgcaca gccggagctg ctcacgacat cgcccgcaca caacaacccg aaacgtctcc
     1981 gatagcaccg gctccgaacc gccgatttct ggacacggag ctcacggtgt gatgaaggtg
     2041 cagccctccc tagcaccgga gacccagaaa gctcgagagt tgccgctctc gggctttcgc
     2101 tatttcagcg ggcgcgcttt ctaccccatc gcgctcggcc cggcccaaat ccgcgccggt
     2161 gaacggtgaa ctgcgcacag cgccgtcccg cgatcttcac cggctacgcg cggatcgcgg
     2221 tgcacgctca catctgtgcg tactcgagct cgagctggcg cctcgggatg agcttcgagc
     2281 tgtttcgtca ggcacttggt ccgtaacccg tgcacgagcg gcgtagggcc gggcgcttgc
     2341 aaaaggagcg gggaaatgcg ccgcaaagtg acgcgcgggc gcgagcgtgc ttggttcgga
     2401 ggcgatgctg cctctggaag gaaccctgct cgagaagctg tgcaagatcg aagcgctgca
     2461 tgctggcacg aaagtcgatg gtgaacgaga agccgcgaag cgcgcggcgg agcgaatccg
     2521 tgcgcggctt gctgagcttc ggagccgtga gcgagagacg gtg
//
LOCUS       DASTKS010000453         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_963523, whole genome shotgun sequence.
ACCESSION   DASTKS010000453 DASTKS010000000
VERSION     DASTKS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963523"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFQ35_09450"
     CDS             <1..1048
                     /locus_tag="VFQ35_09450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930901.1"
                     /translation="AIERTGLHQSSERWALRASDILAESGQRQRARELLGPWLERHAD
                     SVPLLTRAAKLAMAERDFDAAMGAFRKLCKLEQGTARRTAALGLARAAESAGRPEVAV
                     AEVEAVLADGLDSVELRRELARLYARTGDRIKQARMVLDEARAARGSSQLELFTKAAE
                     LFAAEQATDDALAALAELGKLDPERPDAQLLTANVLASAGRIEQAHQTLAAFLKATEK
                     RHTKAHAKLFFRLGELALAQDDWALALPSLLSAHHLDKNDGDIAFTLGLLAADLDELD
                     TAFSTLRAFVTAKDKATDLATRRQLSRACLALAELELAKGQKTVARRMATRAAEADPQ
                     NKEAQRFLTDVALR"
     gene            1086..2003
                     /locus_tag="VFQ35_09455"
     CDS             1086..2003
                     /locus_tag="VFQ35_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930902.1"
                     /translation="MAESIETPQVPAPEPKDSAEVVRCLEAARELWELAQLREAVRWV
                     QRGAEAAEQDGNDMRALTLARKGADLAAHANAVAEKKAEEDSVDVDIDTGEPVEAPPT
                     VPNPAAVSQALQEAIAAVRASQPPPLPPNEITEKSAVATPIPGVTEKSTAATPVPAAA
                     EKSATSTPVPATPEKRASLAPAAASAEKPATRVSEKPGARSFPSPSRPPVTADRPSAR
                     PAATTATASRPPVASVSPKPAVAHVNGVAPNSSVPLAELIASGKAERVTVKRSALDAN
                     VLVVRASQGNKAAHGVRHAVLVYVDEDAG"
BASE COUNT          459 a          905 c          858 g          368 t
ORIGIN      
        1 cgcgatcgag cgcaccgggt tgcaccagtc gtccgagcgt tgggcgctgc gtgcctccga
       61 catcttggcc gaaagcggtc agcgtcagcg cgcgcgggag ctcctcgggc cctggctcga
      121 gcggcacgcc gatagcgtcc cgttgctcac gcgcgccgcc aagctcgcga tggccgagcg
      181 cgatttcgat gcggcaatgg gcgctttccg caagctctgc aagctcgaac aaggcaccgc
      241 gcgccgcacc gcagcgctcg ggctcgcgcg tgcagccgag agcgcggggc gccccgaggt
      301 cgcggtggcg gaggtcgaag cggtgctcgc cgatgggctc gattcggtcg agctccggcg
      361 cgagctcgcg cggctctacg cgcgcaccgg ggatcgcatc aagcaggcgc gcatggtgct
      421 cgacgaggct cgcgccgcgc gcggctcctc gcagctcgag ctcttcacca aggcggcgga
      481 gctgttcgcg gccgagcaag cgaccgacga cgcgctcgcg gcgctcgcag agctcggcaa
      541 gctcgaccca gagcgcccgg acgcccagct cctgaccgcc aacgtgctcg ccagcgccgg
      601 ccgcatcgag caagcgcacc agacgctcgc ggcctttttg aaggcgaccg aaaagcgaca
      661 caccaaagct catgcaaagc tcttcttccg cttgggggag cttgctttgg ctcaagacga
      721 ctgggccctc gcgctgccgt cgctcctgtc tgctcaccac ctggacaaga acgacggcga
      781 catcgccttt acgctcgggc tcctcgctgc tgatctcgac gagctcgata cagcgttttc
      841 gacgttgcgc gcgttcgtta ccgcgaaaga caaagcaacg gatctcgcga cgcggcgcca
      901 gctgtcgcgc gcctgcctag cgctcgcaga gctcgagctc gccaaaggcc agaagacggt
      961 ggcgcgacgc atggcgacgc gcgccgccga ggccgacccg caaaacaagg aagcgcagcg
     1021 attcttgacc gacgtcgcgc tgcgctgaaa ccgactccgt taccgacaga tccaaggaac
     1081 attcgatggc cgaatcaatc gaaacccccc aagtccccgc gcccgagccg aaggattccg
     1141 cggaggtcgt gcgctgcctc gaagcggcgc gcgagctgtg ggagctcgcg cagctgaggg
     1201 aggcggtgcg ctgggttcaa cgcggcgccg aggccgcgga gcaagacggc aacgacatgc
     1261 gagcgctcac cttggctcgc aaaggcgccg atctcgccgc tcatgccaat gccgtcgccg
     1321 agaagaaggc ggaagaggac tcggtcgacg tcgacatcga cacgggagag ccggtcgagg
     1381 cgccgcccac ggtgccgaac ccagcggccg tgagccaggc gcttcaagaa gccatcgccg
     1441 ccgtgagagc ttcgcagcct ccacccctgc cgccgaacga aatcaccgaa aagtcggcgg
     1501 tggctacgcc cattcccggc gtaacggaga agtctaccgc cgcgacgccg gtgcccgccg
     1561 ccgccgaaaa gtcggcgacc tctacgccgg tgccggctac gcccgaaaag cgcgcgagcc
     1621 tcgcgccggc ggcggcttcc gcagaaaaac ctgcgacgcg cgtcagcgaa aagcctggtg
     1681 cccgcagctt cccgagcccc tcgcggccgc ccgtcaccgc cgatcgccct tcggcgcggc
     1741 ccgctgcgac gaccgccacc gcttcgcgcc ctcccgtcgc gtccgtctcg cccaagcccg
     1801 ccgtggcgca cgtgaacggc gtcgccccga acagcagcgt acccctcgcg gagctcatcg
     1861 catccggcaa agcagagcga gtcacggtca agcgctcggc gctcgacgca aacgtgctcg
     1921 tcgtgcgcgc ctcgcagggc aacaaagccg cccacggcgt gcgacacgcg gtgctcgtct
     1981 atgtcgacga agacgctggc tgatccgccg cgcgaaaaca gccaaagcaa ggctgcgagg
     2041 ccgaaacaat agagaaagag cctggctcgt tgcccctcat cgccggcgtg acgcttgcgt
     2101 gaaaccggcg gacttccttg ggcattcgag tcacgcgaag agccgcgtct ggcggttttg
     2161 gtctgggcaa accccgccgc gaagcgaaag gattttgcat gggcgtgccg ccggcccatg
     2221 ttaacgttaa cgtggcggca ggtggtaggc agggtgcttg agagttgcga cagtacggaa
     2281 cgcactggcg aggcaacgcc tcccgagcgg cgcaaagggc tcgttcttcg cggcgtctta
     2341 cccggcgatc gcactgcgat tcagttagat aagtcccgca tcattctcgg acgcgacgcc
     2401 gagtgtgatg cagtgctcgc ggtcgaacga gtgtctcgac ggcatgcgga gatctatcgg
     2461 cagggccccc tggttgcact gcgagatctc gagagcacga acggcacgtg ggttgacggg
     2521 cagcgcactc cccatggcgc ggtgcggccc ggcatgctga ttcgcatcgg ctactggctc
     2581 gggctcgtcg
//
LOCUS       DASTKS010000454         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_294183, whole genome shotgun sequence.
ACCESSION   DASTKS010000454 DASTKS010000000
VERSION     DASTKS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294183"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFQ35_09460"
     CDS             <1..640
                     /locus_tag="VFQ35_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HET9930903.1"
                     /translation="SIFVGTMSNSQTDGGVLSAVMNDATAKPFVKGFGLQWGMIDTYG
                     SLALDKSLPIWQTEHKCGNYPWVNGTNQSKAPNDFAYGVETWGLIRDWIKKGVTSYSA
                     WNMVLDTVGKSVDTVRPWAQNALLTVDTSSKKLNITAAYYVFRHISQYVDVGAKVVGT
                     SGGDALAFKNPDGTIVLVMYNSGAAKKATVKLGGKMLQFDMPDKGWATVNWK"
     gene            884..2428
                     /locus_tag="VFQ35_09465"
     CDS             884..2428
                     /locus_tag="VFQ35_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9930904.1"
                     /translation="MSLLTGFTPWATSKSDVTSEEEKSSVAGEAALTEAPLDPGKSGE
                     RPATSALNTPVPSQRARPERLVYTPAGMSQRVTTAQETVAKPLPIWVDYSTIKVSVVE
                     GPDVGVTRALVNETLRIGSSSDNDLVLADNTVSRHHCAIEPVRGGVRIRDEGSTNGVF
                     VGNLRIFDATVTGTVQLQLGNTRILIEPLSQTVAREQAAGESFGDLLGRSSRMRELFA
                     DLARIAPTDVTLLIEGETGCGKELVAESVHRASKRASAPFVVFDCSAVAPTLIESELF
                     GQVRGAFTSAVARQGLFEQADGGTIFLDELGELPKELQPKLLRVLERREVRRLGAERT
                     TPVDVRVIAATNRNLEAEVRRGNFREDLYFRLATARVRVPPLRDRMDDVPLLVDHFLA
                     RAGSSQKVSDIPRQAWELFMAHRWPGNVRELWNAVQRFLITPERVFSADVASPASGFS
                     PSALPASGPLAPLRVARRDASDAFERAYLEALRARTGTDMKSATAMADVSRQMIQKLM
                     KKHGLE"
     gene            2769..4466
                     /locus_tag="VFQ35_09470"
     CDS             2769..4466
                     /locus_tag="VFQ35_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930905.1"
                     /translation="MSKYRLLARLGGGGMAHAYLGVAEGFEKPYVLKVLRSDVDEREK
                     AELIAMFQDEGKLAARLNHPNIVQSHDMGCDRGEHFIAMEYLAGQPLSLLQERWTEDP
                     ECFPLELRLFVIAQLLDGLDYAHNLADYDGKHLRIVHRDVSQQNVFVTYDGHTKLLDF
                     GIAKTQRSRKTRAGIVKGKVPYMAPEQVKGGELDARADLFSVGVVLWECIANRHFHTD
                     ENEYEILKRVAEGRFPRLRDCVSNVPSQLDKIVSRALAMDPNDRYPDARAFREDLLSF
                     GRVREVSTQDVGRRMQALFARERKVIAEIISVAISRGPDGNADPTTQTALALPFLPTA
                     HTEPKTRDPISTTSPTTAVVDESRISGEARASSLPVASPPAQRSSLPRLLVVGSAGAA
                     MTAAVLAIALGERPVRPAVNANLPASAPKPEALPASKPVAPQSPAPKPAPVDVPAVPA
                     PVASAEQAQLLQLSIVVKPATASVWLDGERIQQIPIHESRVRDGAVHKLRVTAPGYVE
                     RNLSLSFTKDIAETVELVRAASPPPPPPPVPRPEPTWVNPNFGGPTTKGFPRPKDLH"
BASE COUNT          844 a         1481 c         1523 g          840 t
ORIGIN      
        1 atcgatcttc gtcgggacca tgtccaacag ccagacggac ggcggggtgc tctcggcggt
       61 gatgaacgac gctaccgcca agccgttcgt caaaggcttt ggcctgcagt gggggatgat
      121 cgacacctac ggctcactcg ccctcgataa gagcctgccc atctggcaga cggagcacaa
      181 gtgcggcaac tacccgtggg tcaatggcac caaccagagc aaggccccga acgacttcgc
      241 gtacggcgtc gaaacgtggg gcctcatccg cgattggatc aagaagggcg tcacgtcgta
      301 ttccgcatgg aacatggtgc tcgacacggt cgggaagagc gtcgataccg tgcgtccctg
      361 ggctcaaaat gcattgctca cggtcgacac gtcgagcaag aagctcaaca tcacggccgc
      421 atactacgtg tttcgtcaca tctcccagta cgtcgacgtg ggggcgaagg tcgtcggcac
      481 cagcgggggc gatgcgctcg ccttcaagaa cccggacggc accatcgttc tcgtcatgta
      541 caactcgggc gccgcgaaga aagcgaccgt caagctaggc ggcaagatgc tgcagttcga
      601 tatgcccgac aaggggtggg cgaccgtcaa ctggaagtga gccttttgcg ctccgccggt
      661 tcagcgctgc ggtagcaccg gcgaacttcg cgctccgtac cgtattgcgt ggacatcgtg
      721 agcactcggt cgcggctctc gttcgcagat gccgcatcag tgctccgcaa ccgaaccgag
      781 caaccggcgg ttgcgtttgc aactcctggt tgccgcgcga gcgcaaaggc aaagcgcaac
      841 tgcgtcgagc ttggtcggca cggggagtgc tttgggcccg gcgatgtctc tcctgactgg
      901 gtttacgccg tgggccacct ccaagagtga cgttacgtcg gaagaggaga aatcctccgt
      961 ggcgggcgaa gcggctctaa ccgaagctcc gctcgaccct ggcaaaagcg gcgaaaggcc
     1021 agcgacttcg gcgctgaata caccggtccc atcccagcgg gcgcgccccg agcggctcgt
     1081 ctatactccc gccggcatgt cgcaacgcgt gacaacggcg caggaaaccg tcgcgaaacc
     1141 actgcccatt tgggtcgact actcgaccat caaagtgtcc gtggtcgagg gtccggacgt
     1201 tggggtaacg cgcgcattgg tgaacgagac gctccggatc ggtagttcct cggacaacga
     1261 tctggtgctc gcagacaaca cggtttcgcg ccatcactgt gcgatcgagc cggtccgcgg
     1321 cggcgtgcgc attcgcgacg agggctcgac gaacggcgtg ttcgtcggaa atttgcgaat
     1381 tttcgacgcg accgtgactg gaacggtgca gctccagctc ggcaacaccc gtattttgat
     1441 cgagccgctc tcgcagacgg tggctcgtga acaggccgcg ggggagagct tcggcgattt
     1501 gctcgggcgc tccagccgta tgcgggagtt gttcgccgac ttggcgcgca ttgcgccgac
     1561 cgacgtgacg ctgctcatcg aaggcgagac aggctgcggc aaggagctcg tcgccgagtc
     1621 ggtgcatcgg gcgagcaagc gcgcatcggc gccgttcgtg gtgttcgatt gcagcgcggt
     1681 cgcccccacg ttgatcgaaa gcgagctgtt cggccaggtg cgaggcgcct tcacgagcgc
     1741 cgttgcgcgg caggggctct tcgagcaggc cgacggtgga accatcttct tggacgagct
     1801 cggagagctg cccaaagagc tgcagccgaa gttgctgcga gtgctcgagc ggcgcgaggt
     1861 gcgccggctg ggcgccgaac gcacgacgcc ggtagacgtt cgcgtcatcg ccgctacgaa
     1921 ccgcaacttg gaggcggagg tgcggcgcgg caacttccgc gaagacttgt attttcgttt
     1981 ggcgaccgcg cgcgtccgcg tccctccgct gcgcgaccgc atggacgacg tgccgctgct
     2041 ggtcgatcac ttcttggcgc gcgcgggctc ctcgcaaaag gtctccgaca tcccgcgtca
     2101 agcttgggag ctcttcatgg cgcatcgctg gccaggcaac gtgcgcgagc tgtggaacgc
     2161 ggtgcagcgc tttttgatca cgccggagcg ggtcttctcg gcggatgtcg cgagccccgc
     2221 gagcggcttt tcgccgagcg cattgcctgc ttcgggaccg ctcgcaccgt tgcgcgtggc
     2281 gcggcgtgac gcgagcgatg cgttcgaacg tgcatacctc gaagcgttgc gcgctcgaac
     2341 cgggactgac atgaagagcg caaccgcgat ggccgacgtg tctcgtcaaa tgatccagaa
     2401 actgatgaag aaacacgggc tcgaatgacg cggcgccaca ctttcgcgca tgggaatctc
     2461 gctccgagca gctgacgttc gacaggtgcg cccgggggac gaagctcggc cgctgccgag
     2521 gggcggatgc gagccaagcg cgcgaaccgg caaggttctt cgtaagaagc ggggtttttc
     2581 cagtggttct gccggcgaca ttcgccgcga accttggctg gctcgccccg cggcgcgcgt
     2641 ggtaaggtcg cgtccccgcg agaacgtttt cacacgctga tcgttggggc gccggcctcg
     2701 agccgcacgg gcgagcggcc ggaacaacgc cgtcctctcg aagcccagtg tccggtcagt
     2761 atcttacgtt gtccaagtac agactcctcg ccaggttggg tggcggcggg atggcgcacg
     2821 cgtacttggg cgtcgccgag ggcttcgaga agccctacgt gctcaaggtc ttgcgcagtg
     2881 acgtcgacga gcgcgagaag gccgagctca tcgcgatgtt tcaggacgag ggcaagcttg
     2941 ccgctcgttt gaatcacccc aacatcgttc aatcccacga catgggttgc gaccggggtg
     3001 agcatttcat cgcgatggag tacttggcgg ggcagccgct cagcttgctt caggagcgtt
     3061 ggacggagga tccggagtgc ttcccgctcg agctgcgtct gttcgtgatc gcgcaactgc
     3121 tcgatggcct ggattatgca cataacctgg ccgattacga tggcaagcat ctgcgcatcg
     3181 tccaccgtga cgtctctcag cagaacgtgt tcgtcactta cgacggccac acgaagctcc
     3241 tcgatttcgg catcgcgaag acgcagcgtt ctcggaagac tcgcgccggc atcgtcaaag
     3301 gaaaggttcc gtatatggct cccgaacagg tgaagggcgg cgagctcgac gcccgcgcgg
     3361 acctgttcag tgtcggcgtc gtcttgtggg agtgcattgc gaatcggcac ttccacaccg
     3421 acgagaacga gtacgagatc ctcaaacgcg tggcggaggg gcgctttccg cgcttgcgtg
     3481 attgcgtcag caacgttcct tctcagctcg ataaaatcgt gtcgcgggcg ctcgcgatgg
     3541 acccgaatga ccgctacccc gacgcgcgag ccttcaggga agatttgctc agctttggcc
     3601 gcgtgcgaga ggtctccacg caggacgtgg ggcggcgtat gcaggctcta tttgcgaggg
     3661 agcgcaaggt gatcgccgag atcatcagtg tcgcgatcag ccgcggcccg gacggcaatg
     3721 ccgacccgac tacgcaaacg gcgttggcat tgcccttttt gcccaccgcg cacaccgaac
     3781 ccaagacccg cgaccccata tcgactacat cgccaaccac cgccgtagtc gacgaatctc
     3841 gcatctcggg tgaagcgcgg gcatcgtcgc tgccagtcgc ttcgccgcct gcgcaacgaa
     3901 gctcgcttcc tcgcttgttg gtagttggct ctgccggagc ggcgatgacg gcggccgtgc
     3961 tcgcgatcgc gctcggggag cgtccggtgc ggcccgcggt gaacgcaaac ctccccgcga
     4021 gcgcgcccaa gcccgaggcg ttacccgcga gcaagccagt cgcgccacag tctcccgctc
     4081 ccaagccggc cccggtcgat gtacccgcag tcccggcgcc ggtggcttcg gcagagcaag
     4141 cccagctgct tcagctctcg atcgtcgtga agccggcgac cgcgagcgtt tggctggatg
     4201 gtgagcgaat ccagcaaatt ccgattcacg aatcgcgtgt ccgcgacggg gcggtccata
     4261 aactccgagt gaccgcgcca ggttacgtcg agcgaaacct gagcttgtcg ttcaccaaag
     4321 acatcgcgga gacggtcgag ctcgtgcgtg ccgcgagccc gccgccgcct ccaccgcccg
     4381 taccgcgtcc tgaacctacc tgggtgaatc cgaatttcgg cgggccgacg acaaaaggct
     4441 ttccgcgtcc gaaggacctt cactgatttt cgacaccgcg ctcgagagcg tcggcctcga
     4501 gctggacttg gtcaccatga aacgacttca ttcgttcgag cccatgcatc gtctctggtg
     4561 cgtcttcgcg aggccgctcg cggcgagcgc cgcgttcacg atctgtttct cacgggcgcc
     4621 gctcgcgcac gcggacccga acgacggcga agcgcgagct cactatgagc acggggcaca
     4681 gctgtttc
//
LOCUS       DASTKS010000455         6207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_963663, whole genome shotgun sequence.
ACCESSION   DASTKS010000455 DASTKS010000000
VERSION     DASTKS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963663"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFQ35_09475"
     CDS             <1..449
                     /locus_tag="VFQ35_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9930906.1"
                     /translation="LSRFTPRDVPTDAFDFLRREVNRLFSDIEYASPDFEFERGAQWP
                     QVTLEDKGATLVVRADVPGLNEKDLELTLDSSTLTIKGHRRDEVPEGYSTHRKERAAY
                     RFSRSFQLPAKVDPDKTEAALKNGVLTVTLHKAQEAQPRQISVRAT"
     gene            486..833
                     /locus_tag="VFQ35_09480"
     CDS             486..833
                     /locus_tag="VFQ35_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9930907.1"
                     /translation="MTANRFENGQAEPTRASEPRRVTPPVDIFENPAGFLILADLPGV
                     ETSGLNVEFNPPELRVSGRPENAPFVYERRFELGSGVDPTTTSAELRDGVLRIEVKKS
                     EALRPRRIEVRAS"
     gene            complement(982..1599)
                     /locus_tag="VFQ35_09485"
     CDS             complement(982..1599)
                     /locus_tag="VFQ35_09485"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9930908.1"
                     /translation="MAICYYRARLYQLCWASTMRRHIVSFLASIGALGGCELFSPPPA
                     APPPAPQASAAPIASSEAPTPAPEAPPSAQPPPAPETPPPPGIEAQGTGQTRGRLPRA
                     AIQEGVQRAMPAIEGCYLKAAQKKPGVRGRVLVNFVIAPDGSVPYAASLDQGTDFPDN
                     STIDCVLRAFQSLRFPEPVGGRVVATYPLVLEPAPEQSAPQESSK"
     gene            1592..2011
                     /gene="ndk"
                     /locus_tag="VFQ35_09490"
     CDS             1592..2011
                     /gene="ndk"
                     /locus_tag="VFQ35_09490"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9930909.1"
                     /translation="MAIQRTLCIIKPDAVEKGKAGAILQRVLEEGFKIRGLRQVHLSL
                     RQAEGFYAVHRGRPFFNDLCEFMSRGPVVVAALERDEAVQHWRQVIGATDPAKAEPGT
                     IRKLYGAHVGENAVHGSDSEQNGRLECAYFFAEMDLS"
     gene            2285..3064
                     /locus_tag="VFQ35_09495"
     CDS             2285..3064
                     /locus_tag="VFQ35_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930910.1"
                     /translation="MAQLLHSLRVAPKSLSSLLPDVLDGCQPMRASAHSWLLALGDDP
                     RVAVAKRELAAFFAPRIDELEAALREATLRPMNAKTRLTLEDAVSKCSFELDAGRELL
                     ELLEHAVFARPMRLDPRELVREAFTSPPSARAEGRALVSATLSSHDAGGEIEISPRVA
                     MVLVALGVELVGAGPGRERPHVLIASDRQSMCTIRVTRQAHAPGEPLVLASRGVIEPT
                     LASLKAAASLTGGSIEWDSGREEFELSYALAEPALVRDNAS"
     gene            3155..3979
                     /locus_tag="VFQ35_09500"
     CDS             3155..3979
                     /locus_tag="VFQ35_09500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET9930911.1"
                     /translation="MDEKELQTRIEAAYADRSLLKQPGYREAVETVVALLDTGKLRVA
                     TQQGVGAWTTHAWVKQAVLLFFAISEMQKTTAGPFEFYDKIPLKHDLDKFGVRVVPPG
                     TVRYGSFVEPGAIIMPGYVNIGAYVGAGTMVDTWATVGSCAQIGKDCHLSGGVGIGGV
                     LEPPGAQPVIVEDGCFIGSRAIIVEGMLVEKEAVIGAGVALTASTAIIDVTGPEPVEY
                     RGRVPSRSVVIPGMRTKKFAAGEYQIACALIIGQRKESTDKKTSLNAALRDFSVSV"
     gene            3983..5047
                     /gene="dapE"
                     /locus_tag="VFQ35_09505"
     CDS             3983..5047
                     /gene="dapE"
                     /locus_tag="VFQ35_09505"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET9930912.1"
                     /translation="MDLAETLLWLCRTASPIGEERELCDAVLARSACAKTAAPSRRYG
                     DSLVVPLTRGSGGPHIALAGHLDVVRTEHEFAPHVDGDKLYGAGAADMKSGLCLMLDL
                     LEGERPAGVDLTLVFYAREEGPYAENELGRVLDEDRELDGVTLAIALEPSDNKLQLGC
                     GGSLHAQVVFEGRTAHSARPWQGENAIYKARGLLERLSRREPLLDTVDGLVWHEVVSA
                     TLAQGGRARNIVPDRFELNLNHRFGPSTPLERAEKTILDLVNGEARVEFVDRSPAAPP
                     LRNHPLVRALSESGVRAVEPKQAWTDVARFAARGIAAANFGPGVQAQAHQKNEWTSVQ
                     QLFEGREILRSFLQRIGANP"
     gene            5044..5616
                     /locus_tag="VFQ35_09510"
     CDS             5044..5616
                     /locus_tag="VFQ35_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930913.1"
                     /translation="MKQAMVGLCLGISACHAAPTSEGAQRREAIATSARPRDEGPSLY
                     ERGVFERAAQAVLGRLGASTGILMIEVFPERARFEVESTKPGEVVEYEWRHNGVRGPT
                     PLELKGKGSLQQNLFLASSLEFGSLPGLVELARARVDSEQGTVSRVLIRRNLPLDDSV
                     GIRVYVDSPLKSSHVDADARGKPVEPGKYP"
     gene            5727..>6207
                     /locus_tag="VFQ35_09515"
     CDS             5727..>6207
                     /locus_tag="VFQ35_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930914.1"
                     /translation="MTRLVSLLALTVLASACAQSSPERSGALEARPKSYATEPLPAPA
                     PPMADYSAGSGAPAAAAPKAPTARAEAKAGRDYEAESRAADERPGLGTSWGETRESHV
                     SSAPFERRDGDQPFTVSSISYNDAAGVRAMTRGASLGAAPRDGVEVAGGALRIRFLDG
                     "
BASE COUNT         1089 a         2077 c         2055 g          986 t
ORIGIN      
        1 gactttctcg ctttacgcct cgcgatgttc ccaccgacgc attcgatttt ctccgtcgcg
       61 aggtgaaccg gctgttctcg gacatcgagt acgcttcgcc ggatttcgaa ttcgagcgcg
      121 gcgcacagtg gccgcaagtg acgctcgaag acaagggcgc gacgctggtc gtgcgcgccg
      181 acgtccccgg cctcaacgaa aaagatctgg agcttacgct cgacagctcg accctgacca
      241 tcaaggggca ccgtcgtgac gaggttcccg aaggctactc tacgcatcgc aaagagcgcg
      301 cggcgtatcg cttctcgcgg agctttcagc tgcccgcgaa ggtcgatccc gacaagacgg
      361 aggccgctct caagaacggc gtactcaccg taacccttca caaggcccag gaagcgcagc
      421 cgcgccagat cagcgtgcgc gccacctgaa accaccacgg ctttttcttc gacaggaacc
      481 aaaccatgac tgccaatcga ttcgaaaacg gccaagccga accgactcgc gcgagtgaac
      541 cgcgtcgcgt cacccctccg gtagatatct tcgagaaccc cgctggcttt ttgattctcg
      601 ccgaccttcc tggcgtggag accagcggac tgaacgtcga gttcaaccca cccgagctcc
      661 gcgtcagtgg gcgtccggaa aatgctccct tcgtctacga gcgccgcttc gagctcggct
      721 ctggcgtcga tcccacgacc acgagtgcgg agctcaggga cggagtgctc cgcatcgagg
      781 tcaagaagag cgaagcgctt cgcccgcggc gcatcgaagt ccgcgcttca tagcgcggca
      841 cgacgccacc acacccatcg tcccttcgcg acgcggcgca cgcttccctc ccccaccagc
      901 gtgcgtcgtg tcgcacttat ttcgtcacct cgctcgcgcg agcgtgactt cgcgctcgcg
      961 tgaaccgcgc gggacggctg actacttcga gctctcttgt ggcgcgctct gctcgggcgc
     1021 gggctcgagc acgagcggat acgtcgcgac cacgcggccg ccgacaggct ctgggaagcg
     1081 cagcgattga aacgcgcgca gcacgcagtc gatcgtggag ttatccggaa agtccgtgcc
     1141 ctgatcgagt gaggcagcgt acggaacgct tccgtccgga gcgatgacga agtttacgag
     1201 gaccctacct cgcacaccgg gctttttctg agccgccttg aggtagcaac cttcgatggc
     1261 gggcatggcg cgctgaacgc cttcctgaat ggcggcgcgg ggcaagcgac cgcgcgtttg
     1321 cccggtacct tgcgcttcga ttcctggtgg cggaggcgtt tcgggcgcgg ggggcggctg
     1381 ggcgctcggt ggagcctcgg gcgcgggagt cggcgcctcg ctcgaagcga tgggagcggc
     1441 ggacgcctgc ggggcgggag gtggcgcagc cggcggcggc gaaaacagct cgcagccacc
     1501 cagcgctccg atactagcga ggaatgaaac gatgtgacgg cgcatggtag aggcccagca
     1561 aagctggtat aggcgcgctc ggtaatagca gatggccatc cagcgaaccc tgtgcatcat
     1621 caaaccggac gccgttgaaa aaggcaaagc cggcgcaatc ctccagcgcg tactcgagga
     1681 gggcttcaaa atccgtggtc tccgccaagt gcacctgagc ctgcgccaag ccgagggctt
     1741 ctacgcagtg caccgcggcc gccccttctt caatgacctc tgcgagttca tgagccgagg
     1801 cccggtggtc gtagcggcgc tcgagcgcga cgaagccgtc cagcactggc ggcaggtcat
     1861 cggcgcgacc gatccggcaa aggccgagcc cggaacgatc cggaagctct acggcgcgca
     1921 cgtcggagag aacgccgttc acggctcgga ctccgagcaa aacggccggc tcgagtgcgc
     1981 atatttcttc gccgagatgg atttgagctg atcacgagcc gaaacgaggg agctcggctg
     2041 agccgaaagc cgcggccgag ctcgtcccgc cttggggcaa tcctcgttcg tgaacctcga
     2101 aatagcgagc gtgatccacg cgatccaaag cgcctcaccg tgaagaggcg cgcgcccgac
     2161 gaaacccgtg ctagcgtacg tggttctggg aatggatagc gagcgccctc cgccaagttc
     2221 aagggacgca accctggcga gtgcccgagg gaacctacgg gaaacgctag gtgccttgcg
     2281 gaacttggcc cagctgttgc actcgctgcg cgttgcaccg aaatcgctat cgtccctgct
     2341 cccggacgtg ctcgacggct gccagccaat gcgagcgtcc gcccactcgt ggctcctcgc
     2401 cctcggcgac gatccgcgcg tcgcggtagc caagcgggag ctcgccgcct tcttcgcgcc
     2461 tcgcatcgac gagctggaag ccgcgctccg cgaagccacg ctgcgcccca tgaacgcgaa
     2521 gacgcggctc actttggaag acgcggtttc gaagtgctct ttcgagctcg acgccggacg
     2581 cgagctcctc gagttgctcg agcacgccgt tttcgcgcgc ccgatgcgac tcgacccgcg
     2641 tgagctcgtt cgcgaggcct tcacgtcgcc gccgagcgcc cgtgccgagg gtcgcgcctt
     2701 ggtttcggct acgctttcga gccacgacgc ggggggcgaa atcgaaatca gtcctcgcgt
     2761 cgcgatggtg ctggtcgcgt tgggcgtcga gttggtaggt gccgggcccg gccgagagcg
     2821 cccgcacgtg ctcatcgcga gcgatcgcca aagcatgtgt accatccgtg tcacgcgcca
     2881 ggcccatgcc ccgggcgagc ccctggtgct ggccagccgc ggggtgatcg agccgaccct
     2941 tgcgagcctc aaagccgccg cttcgctcac gggcggcagc atcgagtggg actccgggcg
     3001 cgaagagttc gagctcagct acgccctcgc cgagccggcg ctggttcgcg acaacgcgag
     3061 ctgagtcgac catccaggcg tttctccgag tggcgatgcg agctcgagca gcgcgctctt
     3121 gcttcgcgga gtcggcgctc gtaaacccgc ccgcatggac gaaaaagagc tccaaacccg
     3181 catcgaagcc gcctacgccg accgcagcct gctgaagcag cccggttaca gggaagccgt
     3241 cgagaccgtc gtcgcgttgc tcgatacggg caagctgcga gtcgcgacgc agcagggcgt
     3301 tggcgcctgg acgacgcacg cctgggtgaa gcaagccgta ctgctcttct ttgcgatctc
     3361 cgagatgcag aagacgacgg cggggccctt cgaattctac gacaagatcc cgctcaagca
     3421 cgatctcgac aagttcggcg tgcgcgtcgt tcctccgggc acggtgcgct acggctcgtt
     3481 cgtggagcca ggcgcgatca tcatgccggg ctacgtgaac atcggggcgt acgtcggcgc
     3541 agggacgatg gtcgatacgt gggccaccgt cggttcgtgc gcgcaaattg gcaaggactg
     3601 ccatctgtcg ggcggcgtcg ggatcggcgg cgtgctcgag ccgccgggag cgcaacccgt
     3661 gatcgtggag gacggctgct tcatcggctc ccgcgcgatc atcgtcgaag gcatgctggt
     3721 ggagaaagaa gcggtgattg gggcgggcgt cgcgctcacc gcgtccacgg cgatcatcga
     3781 cgtcactggc cccgagccgg tcgagtatcg cggccgcgtg ccttcgcgca gcgtcgtgat
     3841 ccccgggatg cgcacgaaga agttcgccgc gggcgaatac cagatcgcgt gcgcgctaat
     3901 catcggccag cgcaaggaga gcaccgacaa gaagaccagc ctcaacgctg cgctccgcga
     3961 cttttcggtc tccgtctgac cgatggacct cgccgaaacg ctgctctggc tttgtcgaac
     4021 cgcgtcgccc atcggcgaag agcgcgagct gtgcgacgcg gtgctggctc gctcggcgtg
     4081 cgcaaagacg gccgcgccga gccgacgcta cggtgattcg ctggtcgtgc ccctgacgcg
     4141 cggcagcggg ggtccgcaca tcgcgttggc cggtcacctc gacgtggtgc ggacggagca
     4201 cgagtttgct ccccacgtcg acggcgacaa gctctatggc gcgggcgccg ccgacatgaa
     4261 gagcggcctt tgcttgatgc tcgatcttct ggagggcgag cggcccgcgg gcgtcgatct
     4321 gacgctcgtc ttttatgcgc gcgaagaagg gccgtacgcc gaaaacgagc tcggccgcgt
     4381 cctcgacgaa gatcgcgagc tcgacggcgt gacgctcgcg atcgcgctcg agccgagcga
     4441 caacaagctg caactcggct gcggcggctc gctgcacgcc caggtggtct tcgaagggcg
     4501 cacggcgcac agcgctcggc cttggcaggg cgagaacgcc atctacaagg cgcgcggcct
     4561 gctcgagagg ctttcgaggc gggagccctt gctcgacacg gtggacggcc tcgtttggca
     4621 cgaagtcgtg agcgcgacgc tcgcgcaggg cggacgcgcg cgcaacatcg tgcccgaccg
     4681 cttcgagctg aacctcaacc atcgtttcgg cccgagcacc ccgctcgaac gcgccgaaaa
     4741 aaccattctc gacctcgtga acggcgaggc ccgcgtcgaa ttcgtcgacc gctcgccggc
     4801 cgcgccaccg ctacgcaatc accccctcgt ccgcgctttg tccgaaagcg gagtacgcgc
     4861 ggtagagccg aagcaagcgt ggaccgacgt cgcacgcttc gcggcccgcg gcatcgccgc
     4921 cgcgaatttc ggacctggag tgcaagcgca agcccaccaa aaaaacgaat ggaccagcgt
     4981 gcagcagctg ttcgaaggcc gagagatcct tcgaagcttc ttgcagcgga taggcgccaa
     5041 cccatgaagc aagccatggt cggcctgtgc ctgggaattt cggcgtgtca tgctgcgccc
     5101 acgagtgagg gggcccagcg ccgcgaagcg atcgcgacga gcgcgcggcc tcgcgacgag
     5161 gggccaagcc tctacgaacg cggcgtcttc gagcgagccg ctcaagcggt gctcggtcgc
     5221 ctcggtgcga gcaccgggat tttgatgatc gaagttttcc cggagcgcgc gcgcttcgag
     5281 gtcgagtcga cgaagccggg cgaagtcgtc gaatacgagt ggcgtcataa cggcgtgcgg
     5341 ggaccgaccc cgctcgagtt gaagggcaaa ggctcgctgc agcagaactt gttcctggcg
     5401 tcgtctctcg agttcgggag cctgccaggg ctcgtggagc tcgcccgcgc tcgcgtcgac
     5461 agcgagcagg gcaccgtgag ccgtgtcctc atccggcgga acctcccgct cgacgacagc
     5521 gttggaatac gcgtgtacgt ggatagcccc ctcaagagca gccatgtcga cgccgacgcc
     5581 cgtggtaagc ccgtggagcc cgggaagtat ccgtagcaga acccctggaa accgcgcggg
     5641 attccacaat ctgcgcgatc cgctgtaaga gccttcggga acccgcgttg gtttgggcga
     5701 gaccaccgaa ccggagacaa ctcgccatga cccgcctcgt ttcactgctc gccctcaccg
     5761 tcctcgctag cgcttgtgcc caatcgagcc cggagcgcag cggcgcactc gaggcaaggc
     5821 cgaagtcgta cgcgacggag ccgctgccag cgccggcccc tcccatggcc gactactccg
     5881 cgggttcggg cgcgccggcc gccgcagcgc ccaaggcgcc cactgcgcgc gccgaagcga
     5941 aggcggggcg cgactacgaa gcggagtcca gagccgcgga tgagcgcccg gggctcggta
     6001 cgtcgtgggg cgagactcgc gaaagccacg tctcgtcggc gcccttcgag cgacgcgacg
     6061 gcgatcagcc gttcacggtg agctcgattt cgtacaacga cgccgccggc gttcgcgcca
     6121 tgacgcgcgg cgcttcgctc ggggccgcgc cgcgcgatgg cgtcgaagtc gcgggcggcg
     6181 cgctgcgtat ccgcttcctc gatggtg
//
LOCUS       DASTKS010000456         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_72566,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000456 DASTKS010000000
VERSION     DASTKS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72566"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2974
                     /locus_tag="VFQ35_09520"
     CDS             <1..2974
                     /locus_tag="VFQ35_09520"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9930915.1"
                     /translation="AGGNVVEARVFPIAAHERKQIIVAYSEPLLEHTGHVLPLSGLPV
                     LDRLSVRIYDGASPKPTAEFERANYAPDAELRTVLRASAGARVVRAQNLAVMRFTPVN
                     DEKPEALGPTLVLVDTSASQAQSLRRTSERLGDVFRALAQDGDERIIVAAFDQEVQIL
                     HEGTLSSAAGVVGSLLRDRRALGASNLSAALGAATERARKSAIRRVLFVTDGLATAGE
                     REPAALRSMTRATREAGLERIDVISPAGRARETKILAELVSAGLPKAGAVIDDGAGAE
                     AIARKLRSRVAGDLRVDIEGASFVWPQVVRSAQAGDEVVVAAELPANRAVRLTVGGRA
                     IELPAPRTGTAALLKRYVAEARIDALLELEALEGRRGELERRIIELSRQERVLSPYTS
                     LLVLETDADYARFGIARRAPGAYLGVRDGRLALGDSPTLNAATPDRQSRLAVENKDAQ
                     ANEAPQDASRADPPQTSAIGRMWGDEIGESFGAGGLGLSGTGEGGGGEGVTFGMVGST
                     NPTDGVGVGQGFGAGHGRLGGSHATSAPKIRSGTAAVSGRLPPEVIQRIVRQSFGRFR
                     LCYERALAADPNLQGNTVVRFVIGRSGTVEGVVRLAPSLGSHTLDSCISSAFMGLAFP
                     EPEGGKVIVAYPLQLGGGPAATKSAPALAEPPAALEPPLAAAERQRSPESPPAAATYS
                     GRMGEITALLEQRRAPEALNQALEWTSRAPNETLAYVALGKVYAKLERPRDAARAYGS
                     IIDLFSARADMRRYSSNLLESLPERDGLDLAIDSYRVARSDRPDHPSGQRSLALALAR
                     RNRYGEAFDVLLATLARGIQNDRFGGVEHILRDDLALIGALWEAHEPAARQAVHDRLA
                     AAGLEPAHEASLRLVLTWETDANDVDFHIYDAAGNHAFYGNRRLPGGGELYADVTTGY
                     GPECFNIPLTSRAKPSEYRLQAHYYARGPMGYGMGKLEIIEFDGHGSARFEERPYLVM
                     EDRAYADLGSVRVGGERK"
     gene            complement(3026..>3747)
                     /locus_tag="VFQ35_09525"
     CDS             complement(3026..>3747)
                     /locus_tag="VFQ35_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930916.1"
                     /translation="ELARLCGAADEARALFEESVALCRSLGSPAFLELSLGSLARLAR
                     DSAPALGVDVDTAAAEKRGSHREGPDPHVSIERTGDLWAVEGPSQRRFHLKHSKGLAY
                     LSALVASPGHDIHVLALLGIEHRGGDGGPVLDAQARAEYRRRLSALDDEIDEAEKYGD
                     VERARRARAQVESLGAELSRAVGLFGRERRTSSDVERARINVQRRIKDAIDAIERADP
                     GLARYLRATIATGTFCSFTPV"
BASE COUNT          663 a         1249 c         1241 g          594 t
ORIGIN      
        1 ggcgggcggc aacgtcgtcg aagcccgcgt gttcccaatt gcagcccacg agcgcaaaca
       61 aatcatcgtg gcctattcgg agccactgct tgagcacacg ggtcacgtac tgccactcag
      121 cggtctgccc gtgttggaca ggctttcggt gaggatctac gacggcgcaa gtcccaaacc
      181 caccgccgag ttcgagcgcg cgaactacgc gcctgacgcc gaacttcgca cggtgctgcg
      241 cgcttcggca ggcgcacgcg tcgtccgtgc tcaaaatctg gccgtgatgc ggttcacacc
      301 agtgaacgac gagaaacccg aggcactcgg gccgactttg gtattggtcg acacgagcgc
      361 gtcgcaggcc cagagccttc gccgcacttc cgagcgcctc ggcgatgtat tccgagccct
      421 cgcccaagac ggggatgaac gcatcatagt cgccgctttc gatcaggagg tgcagatcct
      481 tcacgaaggg acgctttcga gtgccgcggg ggtggtcggc agtctcctac gcgaccggcg
      541 ggcgctcggc gcttccaact tgagcgccgc cctcggggca gccacagaac gagcacgcaa
      601 aagcgcgatt cggcgtgtac tgttcgtaac ggacggcctc gcgaccgcgg gtgaacgcga
      661 gcccgcagcg ctccgaagca tgacgcgcgc gactcgcgaa gctgggctcg aacgcatcga
      721 cgtcatttcc cctgccggcc gcgcgcgcga aacgaagatt ctcgccgagc tggtctccgc
      781 tggcttgccg aaagcgggcg cggtcatcga cgacggcgcg ggcgccgaag caatcgcacg
      841 caagctgcgc tcgcgcgtcg cgggtgatct ccgggtggac atcgagggcg caagcttcgt
      901 gtggccgcaa gtcgtgcgca gcgctcaggc gggagacgaa gtcgtcgttg cggccgagct
      961 gccagccaat cgtgccgtac gcctcacggt gggcggtcgc gcgatcgagt tgcccgcgcc
     1021 aagaactgga acggcggccc tgctgaagcg ttacgtcgca gaagctcgga tcgacgcgct
     1081 gctcgagctc gaagcgctcg aaggccgacg aggtgagctc gagcgccgaa tcatcgaact
     1141 gtcgcgccaa gaaagggtgc tctcaccgta cacgtcgctg ctcgtgctcg agaccgacgc
     1201 ggactacgca cgcttcggca tcgcgcgccg cgcgccgggc gcataccttg gcgtccgcga
     1261 tggccgacta gcactcggtg actctcctac tctgaacgcc gcaactcccg accgccaaag
     1321 tcgtctcgct gtagagaaca aagatgctca ggccaacgaa gcaccccagg atgcaagccg
     1381 agcagatcca ccccaaacct cggcgattgg gcgaatgtgg ggcgacgaga tcggcgagtc
     1441 cttcggtgcg ggtggattgg gcctcagcgg aactggagag ggcggcggcg gtgagggcgt
     1501 gacgttcgga atggttggca gcacgaaccc aactgatggc gtcggggtgg gccagggctt
     1561 tggagccggg catggtcgtc tcggcggctc gcacgcaacg agcgctccca agattcgcag
     1621 cggaaccgcg gcagtgagcg gcaggcttcc gcccgaggtc atccagcgaa tcgtgcgcca
     1681 gagtttcgga cgctttcggc tgtgctacga acgcgcgctc gcggctgatc cgaacctgca
     1741 gggaaacacc gtcgtacgct tcgtgatcgg tcggagtggg acggtcgaag gggtggtgcg
     1801 cctcgctcca tcgctcggga gtcacacgct ggatagctgc atttcgagtg cgttcatggg
     1861 ccttgctttc ccagagcccg agggcggaaa agtcatcgtc gcgtatccgt tacaactcgg
     1921 cggcggtccc gcggcgacga agtccgcacc ggcgctcgcc gaaccgcctg ccgcgctcga
     1981 gccgcctctc gccgcggccg agcggcagcg gagccccgag agtcctcctg cggccgcaac
     2041 ctacagcggt cgaatgggcg aaatcacggc gctcctcgag cagcggcgag ccccggaagc
     2101 gttgaaccag gcgctcgagt ggacttcacg cgctccgaac gagacattgg cttacgtcgc
     2161 gctgggcaag gtctacgcga agctcgagcg accgcgcgat gccgcgcgcg cctatggctc
     2221 gatcatcgac ttgttttcgg ctcgcgcgga catgcgacgt tactccagca atctgctcga
     2281 gagcttaccg gagagggacg ggctcgatct cgcgatcgat agctaccgcg tcgcccgcag
     2341 cgaccggccc gatcatccgt cgggacaacg ctcgctcgcg ctcgccctcg cgcggcgcaa
     2401 tcgatacggc gaagccttcg acgtgctcct cgcgacgctc gcgcgcggca tccagaatga
     2461 ccggtttggc ggcgtcgagc acatcctgcg cgatgatctc gcgctgatcg gcgcactctg
     2521 ggaggcacat gagccggcag cccgacaagc cgtgcacgac cggctcgccg cagccggcct
     2581 cgagcctgcc cacgaagcgt cgctacgcct cgttctgacc tgggaaaccg acgcgaacga
     2641 cgtcgacttt cacatttacg atgcggccgg caaccatgcc ttctacggca accggcggct
     2701 ccccgggggc ggggagctct acgcagacgt cacgacggga tacggtcccg agtgcttcaa
     2761 catcccactc acgtctcgcg ccaagcccag tgagtatcgg cttcaggctc actattacgc
     2821 acgcggtccg atgggttacg gcatgggcaa gctcgagatc atcgagttcg atgggcacgg
     2881 cagcgcgcga ttcgaagagc gcccatacct cgtgatggaa gatcgcgctt atgccgacct
     2941 gggcagcgtt cgagtcggcg gcgagcggaa gtgagcggcg aacacaacgc tcgagccggt
     3001 gcctgcaaaa cagccgcgat gcgcctcaca cgggcgtgaa agagcagaag gtcccggtcg
     3061 cgattgtcgc acgaagatag cgggccaacc cggggtcagc ccgttcgatg gcgtcgatgg
     3121 cgtccttgat tcgcctctga acattgattc gagcgcgctc cacatccgag ctggtcctgc
     3181 gctcgcggcc gaatagccct acggcgcgcg agagctcggc gccgagcgac tcgacttggg
     3241 cccgggctcg tctcgcgcgc tccacgtcgc cgtacttctc cgcttcatcg atttcgtcgt
     3301 cgagcgcaga caggcgtcgg cggtactccg cgcgcgcttg cgcgtcgagc acgggcccgc
     3361 cgtccccgcc ccggtgttcg atgccaagca gcgcgagcac gtggatgtcg tgtcccgggc
     3421 ttgcgacgag cgcactcagg taggccaacc ccttcgaatg cttcaaatga aagcgtctct
     3481 gcgaggggcc ttcgacggcc cagaggtcgc ccgtgcgctc gatcgagaca tgcgggtctg
     3541 gtccttcgcg atgcgaaccg cgcttctccg cggcagccgt gtcgacgtcg accccgaggg
     3601 ccggagcgct atctcgtgcg aggcgcgcaa gggagccgag cgacaactcg agaaaagccg
     3661 gagacccgag gctcctgcaa agcgcgaccg actcttcgaa gagggcgcgc gcctcgtctg
     3721 cggcgccgca caaacgcgcg agctcgc
//
LOCUS       DASTKS010000457         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_295271, whole genome shotgun sequence.
ACCESSION   DASTKS010000457 DASTKS010000000
VERSION     DASTKS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295271"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ35_09530"
     CDS             <1..432
                     /locus_tag="VFQ35_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HET9930917.1"
                     /translation="TVVIYCYHGHMSQEYAAQFGAFGFSEVYSVDGGFEALASEYAAR
                     RSASEEDERAEPPVRYIVGDVVFAREAIRNDGGIPELEEGALVAPAGTRGVVVQVGRV
                     EADERQTVYAVRFEVAAGELGPMVGCLPEELTQPPATADSR"
     gene            complement(629..1777)
                     /locus_tag="VFQ35_09535"
     CDS             complement(629..1777)
                     /locus_tag="VFQ35_09535"
                     /inference="COORDINATES: protein motif:HMM:NF018155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930918.1"
                     /translation="MSPLPSALESSCVRGQLRVEEHDAITPATLASNQVLRAFLKKTK
                     EGGGRLHLFGLVSDGGVSASLAHLFALIDSAKAASVRVVVHAFLDGIDAPANSARKYI
                     DMLEAKLDGGVGRIGTVSGRTFGMAPEGRWDRVEKVYRAVMADGVDRADSAARGVEAA
                     CVWGVPEQFAAPFVVFDYPGVSLVDTALHFHFAPDGARELVQALAVPEFNRFARKGGR
                     APFAGRFATMTPYETALPIPTLFPRAPDPAELLLDILENPTCTTLTVTSGSAAEITTA
                     AETAIRSGKYDFVFADLANPFNASRGRAETMGSAVTQITAAARDVGGAAMILGGRTGA
                     DAAPIAYVGDSRPQIRDQARTGDLAPTLLDLLGAPRPRHMEGTSLLLF"
     gene            2052..2435
                     /locus_tag="VFQ35_09540"
     CDS             2052..2435
                     /locus_tag="VFQ35_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930919.1"
                     /translation="MARTRTATLAPDCFPDVTALNLALKNERLRVVVDPEWGDAEHNG
                     YVACALDGEDAGFEIARKVSSEGKLVLNVRWGGDRREQAAALGVLSVLASRFGAEVSE
                     PEESKVCSAEELGAQAKSLIQALSE"
     gene            2457..>2716
                     /locus_tag="VFQ35_09545"
     CDS             2457..>2716
                     /locus_tag="VFQ35_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930920.1"
                     /translation="MVKLSGFELFLPVSVTGADARTFMQGQLTNDLRKVTLERAVLAA
                     LCSPQGRVQTMVSVIERAEGLVLLLDRALASDVLSRVQAFTL"
BASE COUNT          442 a          823 c          983 g          468 t
ORIGIN      
        1 acagtggtca tctactgcta ccacggccac atgagtcagg agtatgcggc ccagttcggt
       61 gcgttcgggt tttcggaggt gtacagcgtg gacggcggtt tcgaagcgct cgcgagtgag
      121 tacgctgcgc ggcggagcgc aagcgaggaa gacgagcgag ccgagccgcc cgtgcgctac
      181 atcgtcggtg acgtagtctt cgcgcgcgag gccatccgca acgacggtgg aatccccgag
      241 ctcgaggaag gcgctctcgt cgctccggcg ggtacgcggg gcgtcgtcgt tcaggtgggc
      301 cgggtcgaag cggatgagcg gcagacggtg tacgcggttc gctttgaagt ggcagcaggg
      361 gagctcggac cgatggttgg ttgcctgccg gaggagctga cgcagccgcc cgcaaccgcg
      421 gacagccgct aagtcagggc gagctcgatt ccaatcaggc gagctcgact cgaggggcgg
      481 gcttgtgtct ggcctggcgc gggcttgtgt ctggcctcgt gcgggcttgc gtctagcctc
      541 gcgcgggcct gcgtctagcg tcgcgcgggc ttgcgtccca cctcgcagga gcgcgccgcc
      601 gtgagagcgg gctgacgacg cggtggcttt agaagaggag gagcgaggtg ccctccatgt
      661 gacgagggcg cggcgctccg agaaggtcga gcagcgtggg ggcgaggtct ccggtgcgtg
      721 cctggtcacg aatctgcggg cgcgagtcgc caacgtaagc gatgggcgcg gcgtccgcac
      781 cagtgcgccc tcccaagatc atcgcggctc cgccgacgtc gcgagccgcg gcggtgattt
      841 gcgtgacggc gctgcccatg gtctcggcgc gacctcgtga ggcgttgaat gggttggcga
      901 ggtcggcgaa gacgaagtcg tacttgccgc tccggatcgc ggtttcggcc gcggtcgtga
      961 tctcggctgc gctcccgctc gtcaccgtga gggtcgtgca ggtcgggttc tcgaggatgt
     1021 cgagcaatag ctcggcgggg tcgggcgcgc ggggaaataa cgtggggatc gggagcgcgg
     1081 tttcgtaggg cgtcatcgtc gcaaaacggc ctgcgaatgg cgcacggccg cctttgcgag
     1141 caaagcggtt gaactcgggc acggccagcg cctggaccag ctcgcgcgcg ccgtcgggag
     1201 cgaaatgaaa gtgcagggcc gtgtcgacga ggctcactcc agggtagtcg aatacgacga
     1261 atggggccgc gaattgctcc ggcacgcccc agacgcaagc cgcttcgacc ccgcgcgcgg
     1321 cagagtcggc tcggtccacc ccgtcggcca tgacggctcg atacaccttc tcgacgcgat
     1381 cccaacgccc ttccggagcc attccaaagg tcctgccgga cacggttccg atgcgcccaa
     1441 ccccgccgtc gagcttggcc tccagcatgt cgatgtactt gcgtgcgctg ttcgcgggag
     1501 cgtcgatccc gtcgaggaac gcatgcacga ccacgcggac gctcgcggct ttcgccgagt
     1561 cgatgagcgc gaacaagtgc gcgagcgaag cggagacgcc cccgtcgctg accaacccaa
     1621 acaagtggag tcggccacct ccctccttcg tcttcttcag aaacgcacgc aggacctggt
     1681 tcgatgccaa ggtcgcaggg gtgatggcat cgtgctcctc gacgcgaagc tgtcctcgca
     1741 cacagctcga ttccagggcg ctcgggagcg ggctcatcgc tccctcggtt gcaaccagga
     1801 ccacggggcg gcgcgacgtc gaattgaggc tcatcgggtc cgaaaacagg caaatggtgc
     1861 gccacgtggg cgagtccgcg cgcgcgatgc cgcctcgacg ccgcaagatg gctgaatgac
     1921 cgcgcgtcga ggggaggcgc gtgcggaacg tgtgttcgct tgtcgaccag cttggtccgg
     1981 tttctaacaa cgagggcagg gtttcggcgt ttctggcctc ggtacggggc gtgcttcctg
     2041 cgcgtgactt catggctcgc actcgcaccg cgactctcgc cccggattgt ttcccggatg
     2101 tgacggcgct caacctcgct ttgaagaacg agcgcctacg cgtcgtcgtc gatccggagt
     2161 ggggcgacgc cgagcacaat ggatacgttg cttgtgcgct cgacggcgag gacgctgggt
     2221 tcgagatcgc gcgaaaagtg tcgagtgagg gcaagctggt gctcaacgtg cgctggggtg
     2281 gtgatcgccg cgaacaagcg gcggcgctcg gcgtcctctc ggtgctggcg agccgcttcg
     2341 gggcggaggt gagcgagccc gaagagtcga aggtttgcag cgcggaggag ctcggggcgc
     2401 aagcgaagtc cttgattcag gcgctctccg aatagggcac gggaagaagc gacatcatgg
     2461 tcaaactcag tgggttcgag ctcttccttc cggtcagcgt gaccggagcc gacgctcgca
     2521 cgttcatgca agggcaattg acgaacgatc ttcgcaaggt gacactagag cgcgccgtgt
     2581 tggccgcgct gtgctcgccg caaggtcgcg ttcagaccat ggtgagcgtc atcgagcggg
     2641 ccgaaggcct cgttttactg ctcgatcggg cgctggcgag cgacgtgctc agccgcgttc
     2701 aggcgttcac gctgag
//
LOCUS       DASTKS010000458         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_295654, whole genome shotgun sequence.
ACCESSION   DASTKS010000458 DASTKS010000000
VERSION     DASTKS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295654"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFQ35_09550"
     CDS             <1..587
                     /locus_tag="VFQ35_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930921.1"
                     /translation="SGVYLGTALVISFVTAASLLSSGISDKVPENYRHSLWVHQLFAP
                     GMLLGVAFSLGAGLLGGRSHFRRRVRPLLMARPLPPTPGWSAGYPAQPSPAQPSFGCR
                     VCGAELRSTGDATVTCAYCNSMNLFPASQHEAELADASRRAQSMQHAARHAHVHMVSI
                     ASRMRWIVIAGMALSFVACYALPMLLDKFVFGVK"
     gene            complement(595..1875)
                     /locus_tag="VFQ35_09555"
     CDS             complement(595..1875)
                     /locus_tag="VFQ35_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HET9930922.1"
                     /translation="MADGSKSSPSAALAEAPKSTGAVSRIVAALGLLLRQREFRVVVL
                     CATVMGLAVSFVVPFMSMFGTLEVGMSLGLFGAFMTINTVVNVVISTVLANRSDTRYS
                     RRTMLLVGSGAGALGYLGYAFARQPWQLMLIGSIVLGVASLAFSQLFALSRELLESSE
                     VPPSDAPLYMNAIRMCFALSWTVGPAIAAAALRVFSFKGLFLGAASLYALFFWLTLRY
                     VPETGGSHSRAPAAESLGLGVWLRNPSLLAWFVAFVLILAAHTVSMSNMSLFVLKELG
                     GTEGNVGIIFSLAPIFELPFMLYFGLLATRVESGKLIRVAMALAVVYYVALSCVKAPY
                     QIYPLQILSAAIVSVTSGIAITFFQNKLPDRLGAATNVYSNASRFGSTSAYLLFASSA
                     SRFGHRGTYVLCAVLAALALLLGSVPERREPKQA"
     gene            complement(2017..2304)
                     /locus_tag="VFQ35_09560"
     CDS             complement(2017..2304)
                     /locus_tag="VFQ35_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9930923.1"
                     /translation="MPAPASRRAQEVIKHLGGNVQRLRQKRGYTQERLAEMTRLDLSY
                     TQRVERGQINVTLGTLVTFADALEVPPSALLRPGKGPVIVRGRPTSKRRRV"
     gene            2413..2679
                     /locus_tag="VFQ35_09565"
     CDS             2413..2679
                     /locus_tag="VFQ35_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930924.1"
                     /translation="MKFERLPPMPAKSRLSWQISRLRLDASGRCWLATGVKRELIATI
                     VLPLREHASSEESWLAAIESAFEERARSVFETNTNEVYPITEKP"
     gene            2752..>3039
                     /locus_tag="VFQ35_09570"
     CDS             2752..>3039
                     /locus_tag="VFQ35_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930925.1"
                     /translation="MSDKRSPRYSRRRRATAAAPAQPEGWEERVREQSRNLGRAIEML
                     EQALVLLSEPRTHALARAQCLRGAGLITLIAASELLVMAGWHEADQAVQGGS"
BASE COUNT          581 a          956 c          981 g          521 t
ORIGIN      
        1 tcagtggggt ctacttgggg actgcgctcg tgatctcgtt cgtgacggcg gcgtcgctgc
       61 tatcgagcgg catctctgac aaggtccctg agaattaccg tcatagcctc tgggtgcatc
      121 agctctttgc tccagggatg ctgctcgggg tcgcgttctc tctcggagcg gggttgttgg
      181 ggggacggag ccatttcagg cgccgcgtgc gccctctgct catggcccgc ccgcttccgc
      241 caacacccgg ctggagcgcc ggttatccag cccaaccgag ccccgcacag ccgagttttg
      301 gctgccgtgt gtgcggcgcg gagctgcgtt cgacgggaga cgcgacggtg acgtgcgcct
      361 actgcaattc gatgaacctc ttccccgctt ctcagcacga ggccgagctc gccgatgcaa
      421 gccgccgcgc ccaatccatg cagcatgcgg cgcgacacgc ccacgtacac atggtctcga
      481 tcgcctcacg catgcggtgg atcgtcatcg ccggcatggc gctgagcttc gtcgcttgtt
      541 acgcgctgcc gatgctgctc gacaagttcg tgttcggggt gaagtagcgc gctttcaagc
      601 ctgcttcggc tcgcggcgct ccgggacgga tccgagcaac agcgcgagcg cggccaagac
      661 ggcgcagagc acgtacgtgc cgcgatgacc aaatcgactc gccgagctcg cgaacaacaa
      721 gtacgccgac gtggagccga aacgggaggc gttcgagtac acgttggtgg cggcgccgag
      781 ccggtcgggg agcttgtttt gaaagaacgt gatcgcgatg ccactcgtga cggacacgat
      841 cgcagcgctc agaatttgaa gggggtagat ctggtaaggc gccttcacgc acgacaaggc
      901 gacgtagtag acgacggcca gggccatcgc gacgcgaatc aactttccgg actcgacacg
      961 cgtcgcgagc aggccgaaat acagcatgaa aggtagctcg aagatcgggg cgaggctgaa
     1021 gatgatgccc acgttgcctt cggtgccgcc gagctccttc agcacgaaga gcgacatgtt
     1081 actcatcgac acggtgtgcg cggcgaggat cagcacgaac gcgacgaacc aggcgagcaa
     1141 gctcggattt cgaagccaaa ccccgaggcc cagcgattcg gctgctggtg ctcgcgagtg
     1201 actcccgccc gtctcgggaa cgtaccgcag agtgagccag aaaaagagcg cgtacagaga
     1261 cgccgcgccg agaaacaaac ccttgaatga aaagacgcgc agcgcggccg cggcgatggc
     1321 gggtccgacc gtccacgaca aagcgaagca catgcggatc gcgttcatgt acagcggcgc
     1381 gtcgctcggc ggcacttcgg agctttcgag cagctctcga gagagcgcga acagctgcga
     1441 gaacgcgagc gatgcaacgc ccagcacgat cgaaccaatc aacatcagct gccagggctg
     1501 tcgagcaaac gcatacccga ggtaaccgag cgcgcccgcg ccggagccga ccagcagcat
     1561 cgtgcggcgc gagtaccgcg tgtcggatcg attcgcgagc acggtgctga tgacgacgtt
     1621 gacgaccgtg ttgatggtca tgaaggcgcc gaacagcccg agcgacattc ccacttcgag
     1681 cgtgccgaac atggacatga acggaacgac gaaggagacc gcgagcccca tcacggtcgc
     1741 gcacaatacg acgacgcgga actcacgctg ccgcagcaac agcccgagcg ccgctacgat
     1801 tcgtgacacc gctcccgtgc tcttgggtgc ctccgcgagc gcggcactgg ggctcgactt
     1861 ggatccgtcc gccatgccga ggtggtagct cagatgcgcc ggcttcgctc ttgcgagcga
     1921 tttcgttctt ctctcggcgc gcggctcacg catcgttgcg caacgaattg cgcaaaatgg
     1981 atcacgcgcc ttacgacgac ggcgcgactc gcgctctcag acgcggcgcc gcttgctcgt
     2041 tggacgcccg cgaacgatga cgggcccttt gccgggacgg agcagcgccg agggcggaac
     2101 ttcgagcgcg tcggcaaacg tgaccagcgt cccgagcgtc acgttgattt gacctcgctc
     2161 cacgcgctga gtgtaagaaa ggtcgagtcg cgtcatctcc gctaggcgct cttgcgtgta
     2221 gccacgcttc tgccgcaacc gttgcacgtt gccacctagg tgtttgatga cttcttgtgc
     2281 gcgacgactg gcgggggcgg gcatgtagag gagcgataga tcgggcggtt tccgccaatg
     2341 agggcacgta aatcctcttt agtgttgacc ccgtgacaga acccgctaaa aaagctgcga
     2401 gaggtgggac ccatgaagtt cgagcgtctg cccccgatgc ccgcaaaatc gcggctgagt
     2461 tggcaaatct cacgccttcg tctcgatgcg agtggccgct gttggctcgc gaccggagtg
     2521 aagcgcgagc tcatcgccac tatcgtgttg ccgctgcgtg aacacgcgag ctccgaggaa
     2581 agctggctcg cggcgatcga atccgccttc gaggagcgcg cgaggagcgt cttcgaaacc
     2641 aacaccaacg aggtgtatcc catcaccgaa aagccctgac ggccagtcga agctttactg
     2701 ctcgtccgtt cagtgtatgc tccgcaaatg ctcgactcca gggtgctaac ggtgtccgac
     2761 aagcgttctc cgcgttactc gcggcggcgg cgtgcgaccg cagcggctcc cgcccaaccc
     2821 gaggggtggg aagagcgcgt tcgtgagcaa tcgcgcaacc tcgggcgcgc catcgaaatg
     2881 ctggagcagg cgctcgtgct tttgtccgaa ccgcgcacgc atgcgttggc gcgcgctcag
     2941 tgcttgcgcg gcgcggggct catcacgctc atcgctgctt ccgagctcct cgtaatggct
     3001 ggctggcacg aggccgatca ggcggtccag ggcggctct
//
LOCUS       DASTKS010000459         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_295662, whole genome shotgun sequence.
ACCESSION   DASTKS010000459 DASTKS010000000
VERSION     DASTKS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295662"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1654)
                     /locus_tag="VFQ35_09575"
     CDS             complement(230..1654)
                     /locus_tag="VFQ35_09575"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9930926.1"
                     /translation="MRYGLAVRLGISLAVAAFGGALPAAAEAPSTLKLLDGGGTTTAG
                     LFTASASGAKASLPSGATVTLGPDSAVRAFGRPQRLELERGGKTNTWSLSLTRGRLDV
                     DVPAKPKSAVLVSTEKGSVFVMSGKATLVAEAGELYVVNSAGNVRTYVDNHFADLAAG
                     HIAHFDAEHPRGVDEPVLSAPAIQRGSRLYFSTGAEAKTNDFSWTAVPHAVAYDVELR
                     RGERTIAHRRVNEPRVADLAELAPGAYTVAVRAVDARGIEGSWADARPLRVIGVELPV
                     GAYADGGGIYLAGGQKVKFSHTDGLEMTYLGAGKYLPASESVGLYRNERTVISFRFPG
                     SDDNVIARLEPRDVYAEVFAGPKKAVWPRDPVELHVRMRTRAGGPVPNFLQISPKVEL
                     GVEPVEVEWRRENDDYYARVSPRPGKGPWVIRVDVTDQFGIALGHDFVEVAHQAGASE
                     PPRAAPPTPNDKTSVRVASAKPGG"
     gene            complement(1740..2492)
                     /locus_tag="VFQ35_09580"
     CDS             complement(1740..2492)
                     /locus_tag="VFQ35_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930927.1"
                     /translation="MHRLFAHIGLLPAIALFSVGCKDEATRKCQSQLADAQQVVTAEA
                     TSKAGIERGIAAVDVALAACRAANRSSEVEQLTNARRELAAHIETLNERAGKTRRNKP
                     TPEQVEELIKHGDSSCPRGMAYKAENSDKQIRCTGPQPIRMTFEKARDYYKSLGFRVT
                     ATESPPSLRAEYGAELVLFSFANKDEGPRCLTYYPPPQMPWAEAVARLTGAQLNRIKE
                     NTPVPLSGGDVPLRVDEGKDKLIVYLGSGCSS"
     gene            2714..>3579
                     /locus_tag="VFQ35_09585"
     CDS             2714..>3579
                     /locus_tag="VFQ35_09585"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9930928.1"
                     /translation="MVKGKRADNPLRPRGVTTESGAHARLSSLGSRRAEEFSEPDAEL
                     VALAKAREEGRVHPPPPAEPESAIEALEHRLHELEQELEATRESLRQKQLELGSLRSP
                     DHSSSRSLRIPANPLPAEATEQDVPPLPPPPRSLLPDASGPDSGEWQPASALSVGYPV
                     LQHVAVALRASTLPPAGPDSAPPSQRKAPRRSCEIELAFTEETQFYTGLTQDISEGGV
                     FVATYRLFPIGTRLELSFQLPDGTLVSTWGRVRWLRDASAENRPGMGVEFGELRDDVA
                     RAIARFCEQRAPL"
BASE COUNT          615 a         1238 c         1105 g          621 t
ORIGIN      
        1 tgagctttcc ctccgacgac gtgtcgtcga cgatgatggc ttcgaggcag cgcttgcagc
       61 gcaccgatgt cttacgacca cgaaagcgct cgacgaaaag cgacgtcggg atctgtgatg
      121 ccgctccgca gctcgagcaa gtgatggcga tggacatgac tgcgcagcct tgcacggaag
      181 aacggccgcg ctccgccgat caataggcgc gcgaatcacc gggctcggct cacccgcccg
      241 gcttcgcgct cgcgacgcgc acgctcgtct tgtcgttggg tgtcggcggc gcagcgcgcg
      301 ggggctccga cgcgcctgct tggtgcgcga cctcgacgaa gtcgtgaccg agcgcgatgc
      361 cgaactggtc cgtcacgtcg acgcgaatca cccaggggcc cttgccggga cgcggcgaca
      421 cgcgcgcgta gtagtcgtcg ttctcgcggc gccactcgac ttccaccggc tcgaccccga
      481 gctccacctt cggcgagatt tggaggaagt tcggcacggg accgcctgct cgcgttcgca
      541 tccgaacgtg gagctccacc gggtcgcgcg gccacaccgc cttcttgggt cccgcgaaga
      601 cctccgcgta cacgtcccgc ggctcgagtc gcgcgatgac gttgtcgtcg ctcccgggga
      661 agcggaagct gatgacggtg cgctcgttgc gataaaggcc cacgctctcg ctcgccggca
      721 agtacttgcc cgcaccgagg taggtcattt cgaggccgtc ggtatgagag aacttcacct
      781 tctggccacc ggctaggtag atgccgcccc cgtcggcgta cgctcccacc ggcagctcca
      841 cgccaatcac gcgcaacgga cgcgcatcgg cccagctgcc ctcgataccg cgggcatcga
      901 ccgctcgcac cgcgaccgtg taggcgcctg gcgcgagctc ggcgagatcc gcgacgcgag
      961 gctcattcac gcgtcgatga gcgatcgtcc gctcccctcg ccgaagctcc acgtcgtagg
     1021 cgaccgcgtg aggcaccgcg gtccaagaaa agtcgttggt cttcgcctcg gcgccggtag
     1081 aaaagtagag tcgcgacccg cgctgaatcg caggtgccga gagcaccggc tcgtccacac
     1141 cgcgcggatg ctccgcatcg aaatgcgcga tgtggccagc cgcgaggtcg gcaaagtgat
     1201 tgtcgacgta cgttcgaacg ttacccgcag agttcactac gtaaagctca ccggcttcgg
     1261 cgaccagagt cgccttcccc gacatcacga acacggagcc tttttcggtc gaaaccaaca
     1321 ccgcgctctt cggcttcgcc ggcacgtcga cgtccaaacg ccctcgagtc agagacagcg
     1381 accacgtatt cgtctttccg ccgcgctcga gctccaggcg ttgcgggcga ccaaaggcgc
     1441 gcaccgcgct gtccggcccg agggtgacgg tggctccgct cggtagcgaa gctttcgccc
     1501 cgctcgccga cgccgtgaac agccctgccg tcgtagtgcc gccgccgtcg agcaacttga
     1561 gcgtgctcgg cgcctcggct gcagcgggca gcgcaccacc aaacgccgca accgcaagag
     1621 atatccccag tcgcaccgca aggccatacc tcattccgac gggaacgacc gccaccgtac
     1681 cgactaaagg ctctgcctct tcttttccat cactgcgcgg gactgcgaac gcactccact
     1741 cacgagctgc aaccactccc aaggtagaca atgagcttgt ctttcccttc gtccacgcgc
     1801 agcggcacgt caccgcccga cagcggaacc ggcgtgtttt ccttgatgcg attcagctgc
     1861 gcgccggtga gacgcgccac tgcttcggcc caaggcattt gaggtggggg gtagtacgtc
     1921 aagcaacgcg ggccttcgtc cttgttcgcg aaactaaaca gcacgagttc tgccccgtat
     1981 tctgcgcgca gtgaaggggg cgactcggtc gcggtcacgc gaaagccgag gctcttgtag
     2041 tagtcgcgcg ccttctcgaa cgtcatgcgg atcggctgcg gcccggtgca gcgaatttgc
     2101 ttgtcgctgt tctccgcctt gtaggccatc ccgcgcggac agctcgaatc gccatgtttg
     2161 attagctcct cgacctgctc tggtgtcggt ttgttgcgcc gagtcttgcc cgctcgttcg
     2221 ttcaaggttt cgatgtgggc cgcgagctca cgccgagcgt tggtgagttg ttcgacctca
     2281 ctcgagcgat tcgccgcgcg acacgccgcc agcgcaacgt cgaccgccgc gatgccgcgc
     2341 tcgataccag ccttcgaagt cgcttccgcc gtcaccacct gctgcgcgtc cgcgagctgc
     2401 gactgacact tgcgggtggc ttcgtcctta cacccaaccg agaacagcgc gattgctggc
     2461 aacagcccga tgtgtgcgaa tagacggtgc atttcaacga tccgagtatc attgttgcgc
     2521 acgtttcgcc aaacaatgcc gcgcaactgg cagagatcgg cggcgggcgt ggatgtggca
     2581 gacgcggtga gcgagtggga tgaaaccgag ctcgcctgag attttgtgct ctaccggaac
     2641 gaagaaccgg taccctctcc ttcgtattat cggaggcggt ggtgcgcttc tcggacgccg
     2701 cgaacgatcc accatggtga agggaaaacg cgccgacaat ccgttgcgtc ctcgcggcgt
     2761 cacgaccgag agcggcgccc acgcgcgact ctcgtcgctc ggttcacgcc gcgccgagga
     2821 gttttcggag ccggacgcgg aactcgtagc gctcgccaaa gcgcgggagg aaggtcgagt
     2881 tcatcccccg ccaccagcag agcccgagag cgcaatcgaa gcgctcgagc atcgcctcca
     2941 cgagctcgag caggagctcg aagcgacgcg cgagtcactc cgacaaaagc agctggagct
     3001 cggttcgttg cggtcgcccg atcactcctc gagccggtcg ttgcgcatcc cggcgaaccc
     3061 gctccccgcc gaggccacgg agcaagacgt gcccccgttg cctcccccgc cccgctcgct
     3121 cttgccggac gcgagcgggc ccgattccgg cgagtggcaa ccagcgagcg cgctgagcgt
     3181 cggttacccg gtgttgcagc acgtcgcggt tgcgctcagg gcgagcactc tcccacccgc
     3241 tggaccggac tcagcaccgc cctcacaacg aaaagcacct cgccgctcct gcgagatcga
     3301 gctcgctttc accgaagaaa cgcaatttta taccggcctc acgcaggaca tttccgaagg
     3361 aggcgtcttc gtcgctacct accggctctt tccgatcggg acgcggctcg agctctcgtt
     3421 tcagctcccg gatggcaccc tcgtcagcac gtgggggcgc gtgcgctggc tgcgagacgc
     3481 ttcggccgaa aatcgccccg gcatgggcgt cgaattcgga gagctgcgag acgacgtggc
     3541 gcgcgccatc gcgcgttttt gcgagcagcg cgcgccgct
//
LOCUS       DASTKS010000460         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1412457, whole genome shotgun sequence.
ACCESSION   DASTKS010000460 DASTKS010000000
VERSION     DASTKS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412457"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFQ35_09590"
     CDS             complement(<1..430)
                     /locus_tag="VFQ35_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9930929.1"
                     /translation="MPSEPNPQWKRRRLGERLGRYVLGPALGAGGAACVYLARLDGPR
                     GFERLLAVKLVHEHLLEDRELAAMFLEEARISVLLSHPNIVHTYELGLERDVPFIAME
                     YLHGKPLSAVYQRAFERSEPLAYDLIAWLGARAADALQYAH"
     gene            complement(472..1470)
                     /locus_tag="VFQ35_09595"
     CDS             complement(472..1470)
                     /locus_tag="VFQ35_09595"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9930930.1"
                     /translation="MSCSVFPDHAVLPGTGGSGGGGAGGLSAGGFGNGAHAGTTATSG
                     GLSSGGEPNASGGARPEGGAAGDGGGASPGGEAGMAGATSGGAAQAGESSGGESARGG
                     STGSGGTSNGGTGGANGGNGGATPSGGNGGGGTGTGGQMGTGGVCANPIVLTLGASAD
                     AYVSERSNEKKTNFGQGLGLRVGGAAGAHLHATLAFDLSALGAATKISRATLRVRLGA
                     ATTSSSTLSAYALAQPFVETEVTWERASKASSWVTGGGDTAVSASAMSAVAPSAPAGA
                     TVELDLRGEVSAIASGQRPNNGWLLSVSDGDPNLEFVSRESTFSDERPTLEITSCP"
     gene            1748..2254
                     /locus_tag="VFQ35_09600"
     CDS             1748..2254
                     /locus_tag="VFQ35_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930931.1"
                     /translation="MVRPFFLRKQQRLIALVALAFGASGAPGCRTSCDDAVDSKPDPF
                     SGGQTDPSGSSYQSANWDGHYLKFPPRKVYEFTHHLRAAPFLVVPYVAFSACPYSPRG
                     TCPDDDSPSGQAAVAAGDLAPITQVNETSFRVTNNTCETFYLRVAAYAGSFAEPGSDG
                     DGGAGGGS"
     gene            2262..>2832
                     /locus_tag="VFQ35_09605"
     CDS             2262..>2832
                     /locus_tag="VFQ35_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930932.1"
                     /translation="MDRIKRVLAWMRRRAWDIAFVLLGLALVVWIADDAFGARVVTLS
                     QGADYWEHSATMRELMDHPFSPRNPHLNSAESSPRFVPLFIVLALVARALHWDALNAM
                     GLVATANTLLLVTGIFLFFRSYFRDARASLIGFVVAFGSWYDAWHFSNVYQLKILFST
                     APYPSTTALGLSLLGFTFTLRVLRKGPGRF"
BASE COUNT          421 a          984 c          903 g          524 t
ORIGIN      
        1 cgtgggcgta ttgaagcgcg tctgccgcgc gcgcaccgag ccacgcgatg aggtcgtaag
       61 cgagcggctc gctgcgctcg aaagcgcgct gatacaccgc cgagagcggc tttccgtgca
      121 ggtactccat cgcgatgaac ggcacgtcgc gttcgagccc cagctcgtaa gtgtgtacga
      181 tgttggggtg cgaaaggagc acgctgatgc gcgcctcctc gaggaacatc gccgcgagct
      241 cgcggtcctc gagcaggtgc tcgtgcacga gcttgaccgc caagaggcgt tcgaaaccgc
      301 gcgggccgtc gagccgcgcg aggtacacac aggccgctcc gcccgccccg agcgccggtc
      361 cgagcacgta ccgaccgagc cgctcgccga gtcggcggcg tttccactgc gggttcggct
      421 ctgacggcat caaagccaag cgtaacacga aggcggcccc gagctcggtc gctaagggca
      481 ggacgtgatt tcgagcgtgg gcctctcgtc ggagaaggta ctctcgcgtg agacgaactc
      541 gaggttggga tcgccgtcgc tcacggacag tagccacccg ttgttgggcc tttgcccgct
      601 cgcgattgcg ctgacttcgc cccgaagatc caactccacg gtggctcctg cgggggcgct
      661 cggtgcaacg gcgctcatgg cagaagcgct gacggcggtg tccccaccgc ccgtgaccca
      721 gctcgaagct ttactggcgc gctcccaggt cacctcggtt tcgacgaagg gttgagcgag
      781 cgcatacgca gaaagagtcg acgagctcgt ggttgcggcg cctaaacgga ctcgcaacgt
      841 cgcgcgcgaa atcttggttg cagccccgag ggcgctcagg tcgaacgcaa gcgtcgcgtg
      901 caggtgtgcg cccgcagctc cgccgacgcg cagcccgaga ccttggccga agttggtctt
      961 cttctcgttg gatcgctcgc tgacgtacgc gtcggcgctg gctccgagcg tgagcacgat
     1021 tgggtttgcg cagactccac cggtccccat ttgacctccg gtgccggttc cgccgccacc
     1081 attgcccccg ctcggcgttg ctccgccatt gccgccgttc gcacctccgg tgccgccgtt
     1141 cgacgtgcct ccggagccgg tgcttccccc acgcgcactc tcgccgccgc tgctctcacc
     1201 agcctgcgcg gctccgccgc tcgttgcccc tgccatgccg gcttctccgc ccgggctcgc
     1261 accaccgccg tcgccagcgg cgccgccttc tggtcgagca ccgccagaag cgttgggctc
     1321 gcctccgctc gaaagccctc cgctcgtcgc tgtcgttcca gcgtgcgctc cgttgccgaa
     1381 gccgccagcg ctgaggccac cggcgccccc gcctccgctg ccgcccgttc ccggcagtac
     1441 ggcgtggtca ggaaaaaccg agcaagacat caacaacagc acggcactcc gccatgcggc
     1501 gcaccgccga actcgtcggg tttgctgggc gggcatcggt cctaagccgt tggttccgaa
     1561 agcctgagaa ccatccgccc aagcgtagca acaccccgtt ccaacgacga gggtgccctc
     1621 cgcgggcgag tgagggtagg taagcgcgcc gtccggctcg aggcccgacc ctcgagctcc
     1681 ggtggctttt ttcgcgtggc cgcgctgggc tttgtcagtc gtctaaggtc gtggttaacg
     1741 ttcgtccgtg gttaggccct tcttcttgcg aaagcagcaa cgcttgatcg ccctcgtcgc
     1801 gctcgcgttc ggggcgagcg gtgcgcctgg ctgccgcaca tcgtgcgacg atgcagtcga
     1861 ctcgaagccc gacccatttt ctggcggaca aacggatccg agcggcagct cttaccagag
     1921 cgccaattgg gacgggcact acctgaagtt tccgccgcgc aaagtgtacg aattcaccca
     1981 ccacctgcgc gcggccccgt tcctcgtcgt tccttatgtt gcgttcagcg cgtgcccgta
     2041 ttctccgcgc gggacgtgcc ccgatgacga tagcccgtcc ggtcaggccg cggtcgcggc
     2101 gggcgacttg gctccgatca cgcaggtcaa cgaaacgtcg ttccgagtga ccaacaacac
     2161 ttgcgagacc ttctacctgc gtgttgcggc atacgctggg agcttcgcgg agccgggcag
     2221 cgacggagac gggggcgcgg gcggcggctc ctgaagagtg tatggatcgg atcaaacgag
     2281 ttctcgcctg gatgagaagg cgggcctggg acatcgcttt cgttctcctc gggctcgccc
     2341 tggtcgtctg gatcgcggac gacgcgttcg gagctcgcgt cgtcaccctg agtcagggcg
     2401 ccgactactg ggagcactcc gcgacgatgc gggagctcat ggatcatccc ttttcgccgc
     2461 gcaatccgca cctgaatagc gcggagtcgt cgccgcgctt cgtcccgttg ttcatcgtcc
     2521 tcgctctcgt cgcgcgtgcg ctccattggg acgcgttgaa cgcgatgggg ctcgtcgcca
     2581 cggcgaacac cctgctgctc gtgacaggga tattcctctt cttccgcagc tacttccgcg
     2641 acgcgcgcgc gtcgctaatc ggcttcgtcg tcgcgttcgg aagctggtac gacgcctggc
     2701 acttttcgaa cgtgtaccag ctgaagattc tgttcagcac cgcgccgtat ccttcgacga
     2761 ccgctctcgg tctctcgttg ctcggcttca cgttcacgct gcgcgtgctg cgcaaggggc
     2821 ccggccggtt cg
//
LOCUS       DASTKS010000461         5168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1634630, whole genome shotgun sequence.
ACCESSION   DASTKS010000461 DASTKS010000000
VERSION     DASTKS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634630"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..321)
                     /locus_tag="VFQ35_09610"
     CDS             complement(136..321)
                     /locus_tag="VFQ35_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET9930933.1"
                     /translation="MARLPKGYMSFADFERHEIRPEMRIGWSVDEIEESGKELDFDMD
                     PFEASLWAAENEDKDKE"
     gene            858..1625
                     /locus_tag="VFQ35_09615"
     CDS             858..1625
                     /locus_tag="VFQ35_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9930934.1"
                     /translation="MLRKPDIIAIANQKGGVGKTTTAVNLAASLAAAEKRTLLVDLDP
                     QANASSGVGVLPRTAPRSVYDALIGACSLEEVIQKTGIDLLDVAPSTQDLTAVEYELF
                     EEGRGTHLRELLKDPAIAKYDYVILDSPPSLGVLTLNVLGAAHRVIVPLQPEYYALEG
                     ITYLMATIDRVKTSLNPELTVEGIVLTMWDPRNRLAHQVADQVRKHFRVFEAIIPRNV
                     RLSEAPSHGVPVILYDVGSKGAQGYLSLARELLEVPA"
     gene            1622..2554
                     /locus_tag="VFQ35_09620"
     CDS             1622..2554
                     /locus_tag="VFQ35_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9930935.1"
                     /translation="MSETPKKPLPRGLGRGLDALLPVKAPEPAPGSVAPGSAAPHPAR
                     SYGEGNVFSCPLDKIVPNRTQPRQHFDQEKLEELAQSLREHGLIEPLVVRRRPGTDNF
                     EIIAGERRWRAAQKANLRDVLVVVRDVSAKDAYELALIENVQREDLDPIEFAEALDRL
                     LKEHGHTQETLATRLGKDRSTIANALRLLRLPHAVRERVVNGELSEGHARALLGAPDD
                     ATLAALAEKVVRGRLSVRQTEELVREAKAGKAGKKPGDKAGAGKSASVRDLENRLERR
                     LGTKIDVRDHDGKGEIIVKYASLDDLDRILDILL"
     gene            complement(2646..3254)
                     /locus_tag="VFQ35_09625"
     CDS             complement(2646..3254)
                     /locus_tag="VFQ35_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET9930936.1"
                     /translation="MLSLVDAEAVLEFWFGELDARGAADVDHERRWFQGGSDFDALCR
                     ERFLGVHAAVVAGEAVTWLESPRGRLATLIVLDQLSRNMFRGSSRMFENDLHALRIAN
                     DAIARGDERVLRFAERGFLYLPFEHSESLADQDSCVSLLRAFRNEVGPELAPKVDAWI
                     DFAERHRDIIRRFGRFPHRNVTLERTSTAAELNFLKEPGSSF"
     gene            3533..4042
                     /locus_tag="VFQ35_09630"
     CDS             3533..4042
                     /locus_tag="VFQ35_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930937.1"
                     /translation="MRSRSRVLRLGLTSLLVAGAAGVACSSDGGSDCLNPRPDIPFCN
                     GTGKGGASGLGGAFGSGGLTQASGGQLGRGGAAAAGSPGAGGAVASSGGSLGMGGSTA
                     GGSSGAGGAGVEGGAAGSPAGGESGLGGESGSGGEGGAPVGGAGGATAEAGASGASVH
                     RTPANPLGE"
     gene            complement(4101..>5168)
                     /locus_tag="VFQ35_09635"
     CDS             complement(4101..>5168)
                     /locus_tag="VFQ35_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9930938.1"
                     /translation="YRRSSGEGNDLMIADGTMRCARRSWVLGAVLFSIACSAAPPKQA
                     NTPDPSLEDSSSAPPPGPAQQKAVPASSDKVQKGIDAIQNQDFAAAKSVLTEAEAEAP
                     KDPQPAFYLGVAYEGLGDTASAEKQYRKALALDPKLVDASANLSAILVDAGKTKEALP
                     IIENALKLDPKKSELRVNYALALGAEGRSDEALKAYGDAVESRPDALDLRLAYASLLA
                     KAGKTNESLEQLRTAAKSDDPKLLGAVADAFGKLKAPADCVAVLDRVLKSKPSPALQV
                     RRGVCRHDAGDAKGAHEDFEAALKLDPKFAPAHYYLGMDSKKTDKKAAVAHFQKAAEA
                     GEQDPVGKRAKAELDELKKKR"
BASE COUNT          859 a         1683 c         1762 g          864 t
ORIGIN      
        1 accccgtcgg gaggcaatcc gacggggtca tgggtttctc gcgtccccga agggacgaaa
       61 gtctgttttg gatggggagc taacggccgc agtgcgagac gccggcgtgc gggacgccgg
      121 ggtgccgggc caagctcact ccttgtcttt gtcctcgttc tcggcggccc agagcgacgc
      181 ctcgaagggg tccatgtcga agtcgagttc cttgccgctt tcttcgattt cgtcgacgct
      241 ccagccgatg cgcatctctg gccggatctc gtgccgttca aagtccgcga agctcatgta
      301 gcctttggga agccgtgcca tcgtgcgtca ctctcctcgc ccggttgggc gcaccagcgt
      361 caaatcgcgg tcagcaagcc cggacggagt ccggcggccg cgcacaaacg tgtcgagccg
      421 aaggacggag gcgccggcag tccccacggg tgtgagccag aagccctgct cgaggacccg
      481 cgcgacggac cgttcgaagc gcgcggaccg accgggttca ccagtgcgga cagcggtacc
      541 gagggctgca cgcaaactct ttctgcttgg ctctgcactc actgtgaact acctgcacct
      601 acatctacgc acacaggacg cgacgttctc gatggcgcca aaatttcgtg cgtcgcgctc
      661 acatttcgat aagagccccg caagtgcttt ggcccgagag cggaccgcag tacgtcacga
      721 acccagacca acgaccgagg ctagctgctg agcgccgaag gctttcggtg gtgcccgacc
      781 agttcgagca tccgaaatcc ggacggcgca gctctgccga ggtactaacc tcccgaactt
      841 cgcaaagccc gccccggatg ctccgaaagc ccgacatcat cgcgatcgcg aaccaaaaag
      901 gcggcgtcgg taagacgact acggcagtga acctcgccgc gagtttggcc gccgccgaga
      961 agcgcaccct gctcgtcgat ctcgatccgc aggcgaacgc ttcgagcgga gtcggggtct
     1021 tgccccgaac ggcgccacgc agcgtgtacg atgcgctcat cggcgcgtgc tcgctcgagg
     1081 aggtcatcca gaagaccggc atcgacctgc tcgacgtcgc gccatcgacg caagacctga
     1141 ctgcggtcga gtacgagctc ttcgaggaag gccgcggcac tcaccttcgc gagctgctga
     1201 aggatccggc catcgcgaag tacgactacg tgatcctcga ctccccaccg tcgctcggcg
     1261 tgttgacgct caacgtgctg ggcgccgcgc accgcgtgat cgtaccgctc cagccggagt
     1321 actatgcgct cgagggaatc acgtatctga tggcgacaat cgatcgcgtc aaaacgtcgt
     1381 tgaacccgga gctcacggtg gaaggcatcg tgctcaccat gtgggacccg cgtaaccgct
     1441 tggcccatca ggttgcggat caagtgcgca agcacttccg cgtgttcgag gcgatcatcc
     1501 cgcgcaacgt gcggctctcg gaagcgccgt cgcatggcgt gccggtgatc ttgtacgacg
     1561 tgggcagcaa aggcgctcag gggtatctga gcctcgcgcg cgagctcctc gaggtgccgg
     1621 cgtgagcgag acgccgaaga agccgctgcc gcgcgggctc ggtcgcggcc tcgacgctct
     1681 gctgccggtc aaagcgcccg agccagcgcc cggcagcgtc gcgccgggaa gcgccgcgcc
     1741 gcatccggcg aggagctacg gcgagggcaa cgtcttttcg tgcccgctcg acaagattgt
     1801 cccgaatcgc acccagcctc gccagcactt cgaccaagag aagctcgaag agctcgccca
     1861 atcgctgcgc gaacatggat tgatcgagcc gctcgtcgtg cggcgccgcc ccggcaccga
     1921 caacttcgag attatcgccg gcgagcgtcg ctggcgcgca gcacagaagg cgaacctgcg
     1981 tgacgtgctc gtcgtcgtgc gcgacgtctc ggcgaaggac gcttacgagc tcgcgctgat
     2041 cgagaacgtc cagcgcgaag atctcgatcc catcgagttc gccgaagcgc tcgaccgctt
     2101 gctgaaggag cacggccaca cgcaagagac gctcgccacg cgcctgggca aagatcgctc
     2161 gacgatcgcg aacgcgctac ggctccttcg cctgccgcac gcggtgcgcg agcgcgtcgt
     2221 gaacggcgag ctcagcgaag gccacgcgcg cgcgctgctc ggcgcccccg acgacgcgac
     2281 tctcgccgcg ctcgccgaaa aggtcgtgcg cgggcggctc agcgtgcgtc aaacggaaga
     2341 gctcgttcgg gaagcgaagg ccggcaaagc cgggaagaag cctggcgaca aagcgggcgc
     2401 gggcaagagc gcgagcgtac gtgacctcga gaaccgcctc gaacgaaggc tcgggaccaa
     2461 gatcgacgtg cgcgatcacg acggcaaagg cgagatcatc gtgaagtacg cctcgctcga
     2521 cgacctcgat cgcatcttgg acatattgtt atagggggcg gcacgcgccg gggcgatcat
     2581 gggggcggca cgcgccgccc ccctcgtgcg aagtgaattc gcacgaggct ccccccacgc
     2641 gggctctaaa aggacgagcc gggttccttc aagaagttca gttcggcggc ggtggaggtg
     2701 cgctcgagcg tgacgttgcg gtgggggaag cgcccgaaac ggcggatgat gtcgcggtgt
     2761 cgctcggcga agtcgatcca cgcgtcgact ttcggggcga gctcgggacc aacctcgttg
     2821 cggaaggctc gaagcaacga cacgcagcta tcttgatctg caagcgactc gctgtgctcg
     2881 aatgggagat aaaggaaccc gcgctcggcg aagcgcaaga cgcgctcgtc accgcgcgcg
     2941 atggcgtcgt tggcgatgcg gagcgcatgc aggtcgttct cgaacatgcg cgagctcccg
     3001 cggaacatgt tcctcgagag ctgatccaat acgatgaggg tcgcgagacg cccgcgcggg
     3061 gactcgagcc aagtgaccgc ttcgcccgcg acgaccgcag cgtgcacccc gagaaaccgc
     3121 tcgcgacaga gcgcatcgaa gtcggagccg ccctggaacc agcgcctctc gtggtccacg
     3181 tccgcggctc ctcgagcgtc gagctcaccg aaccaaaact ccaagaccgc ttcggcgtcg
     3241 acgaggctca gcatcaccga aatcctacat cgacggcgat tgctggggcc acccgctagc
     3301 gctctcgtaa gagcttcggc ggcgcgtctc gccggaaatg ctggcggacc tgcttggctg
     3361 gcccgcgtcg ttggctcgga gtggcgggcc tcgctgactc gctctcgcga gcatccttcg
     3421 gctctggatg gagagcctcg gtgggcgggg cgtgccctcg cgcgcggaag agtgctagtg
     3481 ttgcggagtt gttgggtccg ggtgctacac gggccgtcgc ttggagcttc gtatgcggtc
     3541 gcggtctcgg gtcttacgtt tgggtttgac gagcctgctg gtggcgggcg ccgccggggt
     3601 cgcctgctcg tccgatggcg gctcagactg tctcaacccg cgccccgaca ttccgttctg
     3661 caacgggacc ggtaagggtg gtgcttcggg gctgggtggc gcgttcggaa gcggtgggtt
     3721 gacccaagct tcgggcggtc agctgggacg cggtggcgcc gccgctgctg gcagccccgg
     3781 cgcgggcggc gcggtcgcat cctccggtgg cagccttggc atgggcggct ctacagcagg
     3841 tggatcttca ggcgcgggcg gcgccggagt tgaaggcggc gctgcgggga gcccggccgg
     3901 aggtgagagt ggcctcggtg gtgagagcgg ctccggcggc gaaggcggcg ctcccgtcgg
     3961 cggcgctggt ggcgcaacgg ccgaggccgg cgcgagcgga gcctcggtgc accgaacgcc
     4021 cgccaacccc ctcggcgagt aattggcggc gcatcgccgc gctcggaccg cgaacgggac
     4081 cgagcgcggc gcgcgaaaac tcagcgcttc tttttcagct cgtcgagctc ggccttggcg
     4141 cgcttgccca cgggatcctg ctcgcccgct tcggccgctt tctgaaaatg cgcgacagct
     4201 gccttcttgt cggtcttctt gctgtccatg ccgaggtagt agtgagccgg cgcaaacttc
     4261 gggtcgagct tgagcgccgc ttcgaagtcc tcgtgcgccc ctttggcatc gcccgcgtcg
     4321 tggcggcaca cgccccggcg cacctggagc gctggcgacg gcttggactt cagcacgcga
     4381 tcgagcacgg cgacgcagtc cgcaggtgct ttgagtttcc cgaacgcatc ggcgacggcg
     4441 ccgagcaact tgggatcgtc cgacttcgca gcggtgcgga gctgctcgag cgactcgttc
     4501 gtctttcccg ctttggcgag caagctcgcg taggcgagac gcaaatcgag cgcgtcgggc
     4561 cgagactcga cggcgtcgcc gtaagctttc agcgcctcgt cgctgcggcc ctcggccccg
     4621 agcgcgagcg cgtagttgac gcggagctcg gacttcttcg gatcgagctt gagcgcgttc
     4681 tcgatgatgg ggagcgcctc tttggtctta ccggcgtcga ccaagattgc cgaaaggttt
     4741 gcgctggcgt cgacgagctt ggggtcgagc gcgagcgctt tgcggtactg cttttcggcg
     4801 gacgcggtgt cgccgagccc ttcgtaggcg acgccgaggt agaaggccgg ctgcggatcc
     4861 ttcggggctt ccgcttcggc ctcggtgagc accgacttcg ccgccgcgaa gtcttggttt
     4921 tggatcgcgt cgatgccctt ctggaccttg tcgctcgacg ccggcacggc cttctgctgg
     4981 gcggggcccg ggggcggcgc cgacgacgaa tcctcgagcg aaggatcagg cgtgttcgct
     5041 tgcttcggag gcgcagccga acaggcgatc gaaaatagta ctgccccgag cacccagctt
     5101 cggcgggcgc aacgcatcgt tccgtcggca atcattaggt cgttcccttc tccagacgag
     5161 cgacggta
//
LOCUS       DASTKS010000462         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1188430, whole genome shotgun sequence.
ACCESSION   DASTKS010000462 DASTKS010000000
VERSION     DASTKS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188430"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFQ35_09640"
     CDS             <1..340
                     /locus_tag="VFQ35_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930939.1"
                     /translation="RHPPRESRVTTIQGRQRFAWQATLNRQFQVWAQPDGGVAGCPDS
                     QLGGRTFDAWFAESPELPLGPSFSVGFTSRIYYTPHLVGHLVLVVRRRDGGGVVLHVD
                     VVDPTQPAPP"
     gene            812..1465
                     /locus_tag="VFQ35_09645"
     CDS             812..1465
                     /locus_tag="VFQ35_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930940.1"
                     /translation="MASARTFTLEKVDATDALMTSEPAPTEPTVYGAEDFDGAFAEEC
                     GTFHAARTVTITLSAGDWNDAETVEIEGYFGDRDGKPARQFVDVELPAHVDEPTTLRP
                     TQPFYFPPGVTITFPMQPAGGTFTLGVGDAVAPEGFAFDDVRASADGRVSVVYDDEHD
                     DSIEMLRGERLGVRVVAVRAEGTEYGVTLFVPAIGTVEERTQSVDAPGDPHERDRVL"
     gene            1598..2245
                     /locus_tag="VFQ35_09650"
     CDS             1598..2245
                     /locus_tag="VFQ35_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930941.1"
                     /translation="MTGEIVSVDLQALMPATPKRLEYARALRSKLKAPAPKRDGVLAE
                     GTFLPRAVKSKKYLYFHLDQEKEGAAAADLFLHMIWSPRPQRPPPAEVQKRHDEGVTA
                     EVAARAVFGLVAADAEGGNAFSWVDAELLVEGWTPPIGPLAPVLHKGSVLEQVGAEYR
                     APEGATVALKRLRWRREPKNKAKIWLTYWDETDFRYKALWDTVKARCLELLEGLG"
     gene            2341..2631
                     /locus_tag="VFQ35_09655"
     CDS             2341..2631
                     /locus_tag="VFQ35_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930942.1"
                     /translation="MAILRKQREEALQNERDAARAEAQQARAEAQLGLKVARELTVLV
                     AKLNADLQMLKQATVVDPLLTLFPDLDAEDDVAASGNVMPTGLFAAIVSDDE"
     gene            2634..3239
                     /locus_tag="VFQ35_09660"
     CDS             2634..3239
                     /locus_tag="VFQ35_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930943.1"
                     /translation="MTALVGAVPKAKTKYYLDTSAVFALASVQAAKAGQQVPTIETQR
                     ANRLSPFLAKVAVAGGLVCTSVLAFQEVANIARRKARDEFAKQAGFSSWNHLESSAAQ
                     SVQDTVASQAHGAMLQVLEWAAVAVRDANGETEQPAVAADQTKERGQRLRKAHRRFLR
                     AYARLDSMDALHIVIGTELGAKDFISFDKAWESVTEIVVYA"
     gene            3506..3772
                     /locus_tag="VFQ35_09665"
     CDS             3506..3772
                     /locus_tag="VFQ35_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930944.1"
                     /translation="MTTTTVVHALRGELAHRMNQAARAADLLRSAADRLEAEAPDETR
                     SEQAARLRGSSVLAHTAALELAAASGWVEALATAEQNDADGGQS"
     gene            3817..4524
                     /locus_tag="VFQ35_09670"
     CDS             3817..4524
                     /locus_tag="VFQ35_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9930945.1"
                     /translation="MNAPTKPNAPKPAAPARPPATIIKIADLERDPDINCRVGGVNER
                     VAAEYAEAMKAGAKFPPVVVFRDAAGKHRLADGFHRVSAHELAGDTEVRAEVLEGDRR
                     LALLYAAGANAKHGARRTNKDKRRAVLALLADPEWAGKGDAWIAERCAVSDRFVAKLR
                     PATPNGSELKREGRDGKVRKVPKRKARGRKPNPARAVKVARRGLDGVVRGWPKGEPLG
                     PLVEAAKAWLASLEVAP"
     gene            4521..4718
                     /locus_tag="VFQ35_09675"
     CDS             4521..4718
                     /locus_tag="VFQ35_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930946.1"
                     /translation="MTIRREDAVTALRTVRARLDKLASAQLLDAMVTGQLGQMIAGVD
                     VALSFLVRSAPKLRRAKAERK"
BASE COUNT          871 a         1531 c         1642 g          747 t
ORIGIN      
        1 gcggcacccg ccgcgcgagt cgcgcgtaac aacgattcaa ggtcggcaac gctttgcttg
       61 gcaagcaacg ctgaacaggc agtttcaggt gtgggcgcag cccgacggcg gcgtcgctgg
      121 gtgccctgac tcgcagcttg gtggccgaac gtttgacgcg tggttcgccg aatcgccgga
      181 gttgccgctc ggtccgagct tcagcgtcgg gttcacgtcg agaatttatt acacgccgca
      241 cctcgtcggt cacctcgtgc tcgtcgtgcg gcgcagggat ggcggaggtg tcgtgcttca
      301 cgtcgacgtg gtcgatccga cgcaaccggc gccgccgtga cgacggtgcg cacacagaat
      361 cgtccgcgtg gggctttgag ggccttgcac gcggtcgtag gtcgggattt ttcatggccc
      421 gaccgtaggc cggtcgttcc tgccggccga cccgagcaaa ccctggggcg tacgtctagt
      481 acggcgccct ggggcctcgc gttctacaca cttctccgcg cgtcgactca cgacgggcgg
      541 cgaggcggtg ccgtctggtt tcggcactgc gcggtcagcg ccgcccgctt gcgcatcgga
      601 aacgggagcg ggcggcgctg ccgcactaca cacttgcccg gcagagctct gccggtcgag
      661 cacggcgact gcctgggcct ccatacccgg gtgcaagagc gcaacgtcgc gcgctctggg
      721 ctccacttca ggcccgccac gcgcgtcgag gtccctccgt cgatcgcaag gtcgacggag
      781 ggaccgagtt tttcttgtga gagcttgaat catggctagc gcacgcacgt tcacgctcga
      841 aaaagtcgac gcgaccgacg cgctcatgac gagcgagccc gcgccgacgg aaccgacggt
      901 ctacggcgcc gaagacttcg acggggcgtt cgccgaggag tgcggcacgt tccacgccgc
      961 gcgcacggtc acgatcacgc tttcggctgg cgattggaac gacgccgaga cggtcgagat
     1021 cgagggctac ttcggcgacc gcgatggcaa gccggcgcga cagttcgtcg acgtcgagct
     1081 acccgcgcac gttgacgagc cgacgacgct tcgtcccacg caaccgttct actttccgcc
     1141 gggcgtgacg atcacgtttc caatgcagcc agcgggcgga acctttacgc tcggcgtagg
     1201 tgacgccgtc gcgcccgagg gcttcgcgtt cgacgacgtg cgagcgagcg ccgacgggcg
     1261 cgtctcggtc gtctacgacg acgagcacga cgactcgatc gagatgctca ggggcgagcg
     1321 gctcggcgtg cgcgttgtcg cggtgcgcgc ggaaggcacc gagtacggag tgacgttgtt
     1381 cgtcccggcc atcgggacgg tcgaagagcg gacgcaatcc gtggacgctc caggagaccc
     1441 ccacgaacgc gaccgagtgc tatgagaaac gcgccgcagc gtggcaagaa tcgaagcgcc
     1501 gacgtgtcgc cgcaacgcta agcggcgcgt gcgcgccgtt cccggctgga ctctgttctt
     1561 ttcgtcgccc ccgggctaaa ggggctggcc gatgcccatg accggcgaga tcgtcagcgt
     1621 ggaccttcag gcgctgatgc cagcgacgcc gaagcgactg gaatacgcac gcgcgttgcg
     1681 ctcaaagctc aaggctccag ccccgaaacg agacggtgtc ctcgccgaag gaacgttcct
     1741 gccgcgggcg gttaagtcga agaagtacct atactttcac ttggatcagg agaaggaagg
     1801 ggccgccgcg gctgacctct tcctacacat gatctggagc ccacggccac aacgcccgcc
     1861 tccagccgag gtacagaagc gccacgacga gggagtcact gctgaggttg ccgcccgcgc
     1921 cgtgttcggg ttagttgccg cagacgccga gggcggcaac gccttcagct gggttgacgc
     1981 cgaattgctc gtggagggct ggacgccacc gatcgggccc cttgcccccg tgctgcacaa
     2041 aggctcagtc cttgagcaag tcggcgccga gtatagagca ccggagggcg ccaccgttgc
     2101 tctcaagcgg cttcgttggc ggagggagcc gaagaacaag gccaaaattt ggctgaccta
     2161 ctgggacgag acagacttcc gctacaaagc gctgtgggat actgtgaagg cgagatgctt
     2221 ggagcttctg gaaggtctcg gctagagccg cgtcgggggg agtcgcgaag gcgggggtgg
     2281 agcgcttggg atccgcacgc gtatgagcat ccgcgcgaaa agcttgagca cgcgcagcgt
     2341 gtggcaattc ttcgcaagca acgcgaggag gccttgcaaa acgagcgcga cgcagcccgc
     2401 gccgaagcgc agcaagcgcg cgcggaagca cagcttggct tgaaagtggc gcgagagctc
     2461 acggttctcg ttgcaaagtt gaacgccgat cttcagatgc tcaagcaagc aacggtcgtc
     2521 gacccgctct tgactctgtt cccggatctc gacgcagaag acgacgtcgc agcctctggc
     2581 aacgtgatgc cgactggcct ttttgcagca atcgtttccg acgacgaatg actttgacgg
     2641 cactcgtcgg cgcggtgccg aaggccaaga ccaagtacta cctcgacact tctgctgtct
     2701 ttgcgcttgc gtccgttcag gccgcgaagg ctgggcagca agtgccgacc atcgaaacgc
     2761 agcgagccaa ccgactctct cctttcttgg caaaagttgc cgtggccggc ggactcgttt
     2821 gcacgagcgt gctcgcgttc caggaggttg cgaacattgc tcgtcggaag gcgcgcgacg
     2881 agttcgcgaa gcaggcgggg ttctccagct ggaaccatct tgaaagctcc gccgcgcaat
     2941 cagtgcagga cacggtggcg agtcaggcgc atggcgccat gttgcaggtg ctcgaatggg
     3001 ccgcggttgc ggttagagat gcgaacggag aaacggagca acctgcagtc gccgccgacc
     3061 agacaaagga acgcggccag cggcttcgga aggcacaccg ccgcttcttg cgtgcatacg
     3121 cgcgtttgga ttcgatggat gcgcttcaca ttgttattgg aaccgagctc ggtgcaaagg
     3181 acttcatcag ctttgacaag gcttgggaat ccgtgaccga gatcgttgtg tacgcatagc
     3241 cccggcgccg tcacttgacg ggcagatatc tacatgcgac cctgccggta tgagcgggcg
     3301 caacctgcaa cccggcaaga tcctccgtgc ctctgctgag cagtggacgc ggtgggtgaa
     3361 ggccgcgaag cgggccgggc tctcgttcag cgagtgggcg cgccgagttc tcgacgaagc
     3421 cgctcgcaaa ccctgaaaac ccaaagtgtg gggcccgcgg cgctgctaac gcccggaccc
     3481 ctggccaccg aaaggttcgc tttcgatgac gacgacgacc gtagtccatg cactgcgcgg
     3541 cgagctagct caccgcatga accaggccgc gcgcgcggct gatctgctcc gaagcgccgc
     3601 cgaccggctc gaagccgagg cgccggacga gacgcggagc gagcaggccg cccggctgcg
     3661 ggggtcgagc gtgctcgcgc acaccgccgc gctcgaactc gcggccgcga gcggctgggt
     3721 cgaagcgctg gcgacggccg agcaaaacga cgcggacggg ggtcagtcgt gaaggccccg
     3781 agcagcacgg gggcgacgcg gcatcttgac ggggagatga acgcccccac caagcccaac
     3841 gcccccaagc cggcggcgcc tgctcgcccg ccggcaacga tcatcaagat agccgacctc
     3901 gaacgcgatc ccgacatcaa ctgccgagtc ggcggtgtga acgagcgcgt cgccgcggag
     3961 tacgccgaag ccatgaaggc cggcgcaaag ttcccgccgg tggtcgtgtt ccgcgacgcc
     4021 gcgggcaagc accggctcgc cgatggcttt caccgagtga gcgcgcacga gctcgctggt
     4081 gacaccgaag tccgggccga ggtgttggag ggtgaccggc gcctcgcgct gctttacgcc
     4141 gccggcgcga acgcgaagca cggcgcgagg cggacgaaca aggacaagcg tcgcgccgtg
     4201 ctcgcgctgc tcgcggaccc cgaatgggcg ggcaaaggcg acgcgtggat cgccgagcgc
     4261 tgtgcggtgt ccgatcggtt cgtcgcgaag cttcgacccg caaccccgaa cggttcggag
     4321 ttgaagcgcg aaggccgcga cggaaaggtc cgaaaggtcc cgaagcgaaa ggcgcgcggc
     4381 cgcaagccga accccgcgcg cgccgtcaag gtcgcccgcc gtgggctcga tggcgtcgtt
     4441 cgcgggtggc cgaagggcga accgctcggg ccgctcgtag aggctgccaa ggcgtggctc
     4501 gcgagcctcg aggtggcgcc gtgacgatcc gacgtgaaga cgcggtgact gcactgcgca
     4561 cagtccgcgc gcgactcgac aagctcgcaa gcgcgcaact gctcgatgcg atggtcaccg
     4621 gacagctcgg tcagatgatc gcgggcgtcg acgtcgcgct ctcatttctg gtcagatccg
     4681 cgccgaagct gcgtcgtgca aaggcggagc gcaagtgacc gacaagctcg aacgcacgcc
     4741 cgagggtcac aaggtccggc tccgctacgg caacggcctg cggggccggt t
//
LOCUS       DASTKS010000463         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1859022, whole genome shotgun sequence.
ACCESSION   DASTKS010000463 DASTKS010000000
VERSION     DASTKS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859022"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3249
                     /gene="metH"
                     /locus_tag="VFQ35_09680"
     CDS             <1..3249
                     /gene="metH"
                     /locus_tag="VFQ35_09680"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9930947.1"
                     /translation="YTEQIRALIAGGSDVLLVETIFDSLNAKAALVAIQEVFAADGKE
                     LPIMISAAVGRGGETMISAQTIEAFWNAVQHVNPLSVGLNCSLGPDLMYPFLSELSEK
                     STAAISCYPNAGLPNPLSPTGFDLEPKDMARFLGSFADNHLVNIAGGCCGNTPEHIAA
                     IASALVDKPPRDYRGASQVAVPEEFRPLRLSGSQPFTQQKGVFMMIGERTNVAGSPKF
                     AKLIKENKYEEAVSIARQQVENGANVIDICMDEGMIDGVAAMTRFLLLLASEPEVAKA
                     PFMVDSSKWEVIEAGLRCLQGKGIVNSISLKEGEAKFKETARTVLKYGAAVVVMAFDE
                     QGQAATYEDRIRICSRAYRILVDDVGFPPEDIIFDPNILTVATGMEEHNNYAVDFINA
                     TRWIKQNLPHAKVSGGVSNISFSFRGNNPVREAMHAAFLYHAIEAGMDMGIVNAGMLE
                     VYEEIKPELKELVEDVLLNRRPDATERLVEFGEKLKSAGSGATGAEKKEEEWRKGDVR
                     SRLSHALVKGIETYIDGDTEEARKELGRPLAVIEGPLMDGMSVVGDLFGAGKMFLPQV
                     VKSARVMKKAVAYLTPYMEAEKAERAAAGEAVRTQGKIVLATVKGDVHDIGKNIVGVV
                     LACNNYEVIDMGVMVPCEKILERAKQERADLIGLSGLITPSLDEMTHVAREMERQGFD
                     LPLLIGGATTSKAHTAVKIAPHYSKPVVHVLDASRAVPVTTSLLSAETRDTFVKEHQA
                     EYERVRKMHATPKQKLIPLEKARQHRTPIQWRAEDVPQPAFTGLRVLNDFSLATLRDF
                     IDWTPFFHTWELKGVYPRILEHEKYGEQARQLFAEANALLDKIIQNKWLTARGVYGFF
                     PANSRGDDVVLYSDATREKELTTFHFLRQQVDRDENEPSRCLADFIAPRESGLADHIG
                     GFAVTSGVGLKELCDTFRAKHDDYNAIMAEALADRLAEAFAECLHKRVRDEWGYGKAE
                     RLSNSDLIHEKYRGIRPAAGYPACPDHTEKGTLFSLLDAEKNTGIRLTESFAMWPGSS
                     VSGLYFAHPESRYFTVGKIDRDQVLDYHLRKKMSVSEVERWLGPNLNYDPSA"
     gene            complement(3316..3978)
                     /locus_tag="VFQ35_09685"
     CDS             complement(3316..3978)
                     /locus_tag="VFQ35_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9930948.1"
                     /translation="MADSSKPYIIVVGVDFSESGDVALELAFQIADDRYPADVHAVNV
                     VPSFIPSATPDGQSVVGAAFPQSIGDANTRLRNYVEEKLILHQEKGTRRAPSRVITHV
                     RTESAAREVAQLAADLEADLVVVGTNNRKGISRLLLGSTAEATVRLAPCPVLVARPKA
                     APAAEPAIEPPCPRCVQTRIDTNGAEYWCEQHRERHGQRHTYHQGDRVSSDNSMPLVM
                     HG"
     gene            4252..4485
                     /locus_tag="VFQ35_09690"
     CDS             4252..4485
                     /locus_tag="VFQ35_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930949.1"
                     /translation="MPTKFALIAAAAAGIIAGAACSSQKPAEDPSTSSIASTEHHACK
                     GQNACKGQGGCKTEQHACKGQNLCRGQGGCKVG"
BASE COUNT          900 a         1481 c         1454 g          757 t
ORIGIN      
        1 tacacggagc aaatccgcgc cctcatcgcc ggcggcagcg acgtgctctt ggtcgagacc
       61 atcttcgatt cgctgaacgc caaggcggcc ctcgtcgcga ttcaagaggt cttcgcggcg
      121 gacggcaaag agctcccgat catgatttcg gcggcggttg gccgcggcgg cgaaaccatg
      181 atttcggcgc agacaatcga agcgttttgg aacgccgtgc agcacgtgaa tccgctgtcg
      241 gtcgggctga attgctcgct cggccccgac ctcatgtacc cgttcctctc ggagctctcc
      301 gagaagtcga cggctgctat ttcttgctac ccgaacgcgg gcttgccgaa tccgctctcg
      361 cccaccggct tcgacctcga gccgaaggac atggcgcgct tcctcggcag cttcgccgat
      421 aaccacctcg tgaacatcgc ggggggctgc tgcggcaata cgcccgagca catcgccgcg
      481 atcgcgagcg cgctcgtcga caagccgccg cgtgactatc gcggcgcttc gcaggtggcg
      541 gtgccggaag agttccgccc cctgcggctc tccggctcgc agccgttcac gcagcaaaaa
      601 ggcgtgttca tgatgatcgg cgaacgcacc aacgtcgccg ggtcgcccaa gttcgcaaag
      661 ctcatcaaag agaacaagta cgaggaggcg gtctccatcg cgcgccaaca ggtggagaac
      721 ggcgcgaacg tcatcgacat ctgcatggac gaggggatga tcgacggcgt cgcggccatg
      781 acgcgcttcc tcttgctgct cgcgagtgag cccgaggtcg cgaaggcccc gttcatggtg
      841 gactcctcca agtgggaggt catcgaggcg ggcctgcgct gcttgcaggg caaaggcatc
      901 gtgaactcga tttcgctgaa ggaaggcgaa gcgaagttca aggaaaccgc gcgcacggtg
      961 ctcaaatacg gcgctgcggt ggtcgtgatg gccttcgacg aacaggggca ggcggcgacc
     1021 tacgaagacc ggattcgcat ctgctcgcgc gcgtaccgca tcttggtcga cgacgtcggc
     1081 ttccctccgg aagacatcat cttcgacccc aacatcctca cggtcgcgac cgggatggag
     1141 gagcacaaca actacgccgt cgacttcatc aatgcgacgc gctggatcaa gcagaacctc
     1201 ccccacgcca aggtgagcgg cggcgtctcc aacatttcgt tcagcttccg cggcaacaac
     1261 cccgtgcgcg aagcgatgca cgccgccttt ctgtatcacg ccatcgaagc cggcatggac
     1321 atgggcatcg tgaacgccgg catgctcgag gtgtacgaag agattaagcc cgagctcaag
     1381 gagctggtcg aagacgtgct cctcaaccgg cggcccgacg cgaccgagcg cctcgtcgag
     1441 ttcggcgaaa agctcaaatc ggccggcagc ggcgcgaccg gcgccgaaaa gaaggaagag
     1501 gagtggcgga agggcgacgt ccgctcacgc ctttctcacg cgctcgtgaa gggcatcgaa
     1561 acgtacatcg acggggacac cgaagaagcg cgcaaagagc tcgggcgacc gctcgccgtc
     1621 atcgagggtc cgctgatgga cggcatgagc gtcgtcggcg acctgttcgg cgccggaaag
     1681 atgttcctgc cgcaagtcgt gaagtccgcg cgcgtgatga aaaaggcggt cgcgtacctc
     1741 acgccgtaca tggaggccga aaaggcggag cgcgcggccg cgggcgaagc ggtgcgcacg
     1801 caaggcaaaa tcgtgctcgc caccgtgaag ggcgacgtgc acgacatcgg caagaacatc
     1861 gtcggcgtcg tgctcgcttg caacaactac gaggtcatcg acatgggcgt gatggtgccc
     1921 tgcgaaaaga tcttggagcg agcgaaacaa gagcgggcgg atctcatcgg gctctcgggg
     1981 ttgatcacgc cttcgctcga cgaaatgacg cacgtcgcgc gcgagatgga gcgccaaggc
     2041 ttcgatttgc cgctcttgat cggcggcgcc acgacgagca aagcccacac ggccgtgaag
     2101 atcgcgccgc actacagtaa gcccgtggtc cacgtgctcg acgcgagccg cgccgtaccg
     2161 gtcaccacga gcctgctcag cgcggagaca cgcgacacct tcgtgaagga gcaccaggcc
     2221 gagtacgagc gcgtgcgaaa gatgcacgcg accccgaagc agaagctgat tcctctcgaa
     2281 aaagcgcggc agcaccgcac tccgattcag tggcgcgccg aggacgtacc gcagccggcc
     2341 ttcacgggcc tgcgcgtgct caacgacttt tcgctcgcga cgctacgcga cttcatcgac
     2401 tggacgccgt tcttccatac gtgggagctc aaaggtgtgt acccgcgcat cctcgagcac
     2461 gaaaaatatg gcgagcaggc gcggcagctg ttcgcagaag cgaatgcgct gctcgacaag
     2521 atcatccaaa acaagtggct cacagcgcgc ggcgtatacg gcttcttccc cgcgaactca
     2581 cgcggtgacg acgtggtttt gtacagcgac gctactcgag agaaggagct caccacgttc
     2641 cacttcttgc gccagcaggt ggaccgcgac gaaaacgagc cttcgcgctg cctcgccgac
     2701 ttcatcgctc cgcgcgagag cgggctcgcc gatcacatcg gtggtttcgc ggtcacgagc
     2761 ggcgtcgggc tcaaggagct gtgcgatacg ttccgcgcga agcacgacga ctacaacgcc
     2821 atcatggccg aagcgctggc agaccgcttg gcagaggcct tcgccgagtg cctgcacaag
     2881 cgcgtgcgcg acgagtgggg ctatggaaaa gccgagcgcc tcagcaactc cgacctgatc
     2941 cacgaaaagt accgcggcat tcgcccggcg gctggctacc cggcgtgccc cgaccacact
     3001 gaaaagggca ccctattcag cctgctcgat gcggagaaga acacgggcat ccgcctgacc
     3061 gagtcgttcg cgatgtggcc cggctcgagc gtcagcggcc tctactttgc gcaccccgag
     3121 tcgcgctact tcaccgtcgg caagatcgac cgcgaccaag tgctcgacta ccaccttcgc
     3181 aagaagatga gcgtgagcga agtcgagcgc tggctcgggc caaacctgaa ctacgacccc
     3241 agcgcgtgaa cgcagtcgtc gcgggggacc gacgcgcggt ggagttcgcc gcgcgtcggc
     3301 cgtgcacgcc gcgcgtcagc cgtgcatgac gagcggcatc gagttgtcgc tgctcactcg
     3361 gtcgccttga tgatacgtgt gtcgctgccc gtgacgttcg cggtgctgct cgcaccaata
     3421 ctcggcgccg ttcgtgtcga tgcgcgtctg cacgcagcgc gggcacggcg gttcgatggc
     3481 cggctctgcg gcgggcgctg ccttcggccg tgcgacgaga accgggcaag gcgcgagccg
     3541 cacggtggcc tccgcggtcg aacccagtag cagccgcgaa atcccttttc ggttgttcgt
     3601 gccgaccacg acgagatctg cctcgagatc cgcagcgagc tgggcgacct cgcgagccgc
     3661 cgactcggtg cgcacgtgcg tgatcacgcg ggaaggcgcg cgacgcgtgc ccttctcttg
     3721 gtgcaaaatt agcttttcct cgacgtagtt gcgaaggcgc gtgttggcgt cgcctatcga
     3781 ctgcgggaag gcggcgccga ctacggattg cccatccggc gtcgcgctcg ggatgaaact
     3841 cggaacgacg ttcacggcgt gaacgtcggc gggatagcga tcgtcggcga tctgaaatgc
     3901 gagctccagc gcgacgtcgc cgctctcgga gaaatcgaca ccgaccacga tgatgtacgg
     3961 cttgctgctg tcagccatgt tcgttctcct cgtctcatcg cgaagcaagg cgtgtgcctg
     4021 ccctaattgc gccgctttca tggactattc actggatgtt tcgagatcgg ccacgtgtca
     4081 gctttgcgct ttgaggcacg agcgtagggt gccggttctt tcggcgcccg acgtcaccca
     4141 tcgctgtaac cggcgacgcc cctcttcgta gggagcaacg aactcctcac gccttcgctc
     4201 gaatcgagcg agagcgggat ttcactcgtc ctcaatcgaa agagagtatc catgcccacg
     4261 aaattcgccc tcatcgccgc tgccgccgcc ggaatcatcg ctggagccgc ttgctcttcg
     4321 caaaagcccg ccgaagaccc gagtacgtcg agcatcgcgt ctaccgagca ccacgcctgt
     4381 aagggacaga atgcctgcaa gggccagggc ggctgcaaga ccgaacaaca tgcctgcaag
     4441 ggccagaatc tctgccgcgg ccagggcggc tgcaaagtcg gttgaacgtc tcgggggcaa
     4501 ggccaacaac cgggctcagc gacggacgcc aacaccgtac gcccggaggt gcggtacctt
     4561 ctgctccgtg ctgctgaacg aacgtaccga cg
//
LOCUS       DASTKS010000464         4980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_520045, whole genome shotgun sequence.
ACCESSION   DASTKS010000464 DASTKS010000000
VERSION     DASTKS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520045"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1007)
                     /locus_tag="VFQ35_09695"
     CDS             complement(309..1007)
                     /locus_tag="VFQ35_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930950.1"
                     /translation="MKSPRIVTHLFADGGRIIKTARTSYAELVGRSDLAQTVRQMMKE
                     QHRSMFASLRAGELDAMLEAACGPLPLPPVERSEAAERPAASLRDPATAPQPEPAAVS
                     SPKPVASAPSPAARSLSNPNLHRIPPSVPPPSREDLEIAVGAVEKLSVESPSATMPVA
                     NRTDSARARKSTPPGARKSRTPAVPSVATRPSARAASSKPAQSIFGDSVISEKSLDEV
                     ILSYLAEDLDGPSE"
     gene            complement(1404..3308)
                     /locus_tag="VFQ35_09700"
     CDS             complement(1404..3308)
                     /locus_tag="VFQ35_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9930951.1"
                     /translation="MADKLVTPEQQEAALNLAARSGDRIEEILLEMKALEEAQLLKFL
                     ASLHRTRFVTTEKLAKAEINRQTLDKVPKKLAERDCVFPVLYDTQTATLSVVTPDPDN
                     AAMLQDVQLASGVKDVRAFVGRPRAVRAAIQKAYNGDIHAFATLDRDAHAQFSTMLNV
                     FERNLVSEESLAVAVGKDAVAERVFTSADLERGGKAATSGGKGVTDVAFLETLNVLVT
                     LLENGRQDLRGHSAQVARLMKKIGERIGVSEVARAAMQVAGYLHDIGKMGAYHLTSLN
                     VAEYEGHRTSAGKLYKAPLRLLEAVNLPTEVTQTIEHMYERYDGQGLPDRTAGKEIPL
                     GARLLAIADTYADLTQNPRNPFRKTLRPTQACEVLDRYKGTIFDPNLVDLFKHTVTGE
                     DLKARLLANRHRALLLDPDPEDTTVLELRMIEQGFEVLLARSVEQAQKMLEKGDVEIV
                     ISEIDLPPTDGFELLAAARQKPWGQKLPWVYVTSRSGRTDAQKAFELGAADFMTKPIA
                     ADVLVAKLRQILEREASRGGASRGVAGSLLEMGLPDIVQVLWHGRKSGSLKIRAGANA
                     GEIHFVDGAIYNALYSNLRGEEAFYAMLALNEGDFVLDPNFRAPQQVVQASPEALLLE
                     GMRRLDESGR"
     gene            3577..4011
                     /gene="rplM"
                     /locus_tag="VFQ35_09705"
     CDS             3577..4011
                     /gene="rplM"
                     /locus_tag="VFQ35_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9930952.1"
                     /translation="MPTYVAKPAEAQATRKWYVVDATDQPLGRLASKIAAVLRGKHKP
                     TYTPHVDTGDFVIVVNAAKVKLTGRKTTDKMYYRHSGYPGAIRGESFEHVVQRHPDAP
                     ITLAVKGMLPKNVLGRQMLEKLKVYGNGDHPHRAQKPESLNV"
     gene            4034..4429
                     /gene="rpsI"
                     /locus_tag="VFQ35_09710"
     CDS             4034..4429
                     /gene="rpsI"
                     /locus_tag="VFQ35_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9930953.1"
                     /translation="MSEAQSTYATGKRKTAVARVWIQPGSGAVTVNGASIDNYFERET
                     SRMVVRQGLELLELHDKYDVVASVCGGGHSAQAEAMRHGIARALTSEDPERRRALKRA
                     GYLTRDDRKKERKKYGQAGARKRFQYSKR"
     gene            4433..>4980
                     /locus_tag="VFQ35_09715"
     CDS             4433..>4980
                     /locus_tag="VFQ35_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930954.1"
                     /translation="MLKVGIIGAGGYTGAELVRLTYAHPELELELVAAREKAGKRLGE
                     VLPSVLGVKALEDRLLETFDPEQAVALKSRIDVAFLALPHGASARAGKALFDAGIQVV
                     DLSADFRLKDAEAYRRTYAEHPAPELLRTAVYGQPELHRDELRDAKLIAAPGCHVTTA
                     LLPLAPLLKAKLIDPNGIVIDS"
BASE COUNT          875 a         1592 c         1634 g          879 t
ORIGIN      
        1 caccggtcca ggtctcgctg ccacttctcg ctgaggtcgc gcatcgcgct cgcctatgga
       61 gcacacggag cgccgaatgg aaagagaaaa gtggcggagt agtgctagaa cgccgatcag
      121 tttgactgct cggcgttcta gtcttggttt gtgggggcgg cacgcgccgc ccccctcttc
      181 cgaaatgaat tcggaagaga ctccccccac gcgggcactc cgtgcggagc cgttttccgc
      241 taccttcggc gaattcgtaa ccgtttgtcg ctctagccgg gcgttgaaaa acgccctgct
      301 aggctccgct actcggaggg gccgtcgagg tcctcggcga gatagctgag gattacttcg
      361 tccagcgact tttcgctgat cacgctgtcg ccgaagatcg actgagcggg cttcgacgaa
      421 gccgcacgcg ccgacggccg tgtcgccacc gatggtacgg ccggtgtgcg cgacttgcgg
      481 gctcccggtg gtgtgctctt tcgagcgcgg gccgagtcgg tgcggttcgc caccggcatc
      541 gtcgcagacg gcgactcgac gctgagcttt tcgaccgcgc cgacggcgat ctccagatcc
      601 tcccgcgagg ggggcggcac cgacgggggg atgcgatgca agttggggtt cgagagcgac
      661 cgagcggcgg ggctcggcgc cgacgcgact ggcttcggcg aggagacggc ggctggttcc
      721 ggctgaggcg cggtcgccgg gtcgcgtaac gacgcggcag ggcgttcggc ggcctcgctg
      781 cgctcgacag gtggaagcgg cagaggaccg caagcagctt cgagcatcgc gtcgagctca
      841 ccggcgcgca acgacgcgaa catcgaacgg tgctgctcct tcatcatctg gcgcaccgtc
      901 tgcgcgagat cactgcggcc cacgagctcc gcataagacg tgcgcgccgt cttgatgatg
      961 cggccaccgt ccgcgaaaag atgggtcacg atgcgcgggc tcttcacgcc cgagtcttcg
     1021 gtttgaatat ggaaaacgcg accgcgatgg cggacgttat tattgaagcc gaggactggg
     1081 gggggctgtt gggacacgct tgggccctgc aaactcagta gtttaacacg acggcacgag
     1141 aacaggccca aatcgagtgc gcgagcgctc gttcacgtag acgtgagtat atgaacaggg
     1201 gcctttcggc gttcgccaga gcttcggtgg ttccagagcc cacacgaatt ctcgcgcaat
     1261 ttacggcaaa cgcgcggctc tccgtcggcg aaaggccgca cgagacgcac atcgtgccat
     1321 ctctcgaggg tggcgtcgct gccgaggttt cgcccaggga gcgatgggcc gccgcttcac
     1381 ccgcgagcac gagggagctc gacttagcgg ccggactcgt cgaggcgtcg catcccctcg
     1441 agtaagagcg cctcggggct cgcctgcacc acctgctgcg gcgcgcggaa attcgggtcc
     1501 aatacgaagt ctccctcgtt cagggcgagc atcgcgtaaa aggcttcttc gccgcgcagg
     1561 ttcgagtaga gcgcgttgta gatcgcgccg tcgacgaaat ggatttcgcc ggcgttcgcg
     1621 ccggctcgga ttttgagcga gcccgatttt cgcccatgcc acagcacctg gacgatgtcc
     1681 ggcaggccca tttcgagcag ggatccagca actccgcgtg acgcgccacc ccgagaagct
     1741 tcgcgttcca agatttgacg cagcttggca acgaggacgt cggccgcgat cggcttcgtc
     1801 atgaagtccg ccgcgccgag ctcgaaggcc ttttgagcgt cggtgcgacc ggagcgcgac
     1861 gtcacgtaca cccacggaag cttctgcccc cacggcttct ggcgcgccgc ggcgagcaac
     1921 tcgaacccat cggtgggcgg caagtcgatc tcgctgatga cgatctcgac atcacctttc
     1981 tcgagcatct tttgggcctg ctcgaccgat cgcgcgagca gcacctcgaa gccctgctcg
     2041 atcatccgga gctcgagcac ggtcgtatct tcggggtcgg gatcgagcaa gagcgctcgg
     2101 tggcggtttg cgagcagcct cgccttgaga tcttcgccgg tgaccgtgtg cttgaagagg
     2161 tcgaccaaat tcggatcgaa gatcgtgccc ttgtaacggt cgagcacctc gcaagcctgc
     2221 gtcgggcgta gcgtcttgcg gaaggggttg cgcggattct gagtgaggtc ggcgtacgtg
     2281 tcggcgatgg ccagcaagcg cgctccgagc gggatctctt tgcccgcggt gcgatcgggc
     2341 aggccctggc cgtcgtagcg ctcgtacatg tgctcgatgg tttgggtgac ctcggtcggc
     2401 agattcaccg cttcgagcag gcgcagggga gccttgtaga gcttgccggc ggaggtgcgg
     2461 tggccttcgt attcggcgac gttgagcgac gtgaggtggt aagcgcccat cttgccgatg
     2521 tcgtgcaggt agcccgcgac ctgcatcgcc gcgcgcgcca cttcggaaac gccgatgcgc
     2581 tcgccgatct tcttcatcag ccgcgcgact tgcgccgagt ggccgcgcaa atcctggcga
     2641 ccgttctcga gcagggtgac gagcacgttc agcgtctcga ggaacgcgac gtcggtaacg
     2701 cccttgccgc cgctcgtcgc ggctttgcct ccgcgctcca gatccgcgct cgtgaacacg
     2761 cgttcggcga ccgcgtcctt cccgacggcg acggccaagc tctcttcgct gaccaagttg
     2821 cgctcgaaca cgttcagcat cgtcgagaac tgcgcgtgag cgtcgcggtc gagcgtcgcg
     2881 aaagcgtgga tgtctccgtt gtaagctttt tggatcgccg cgcgcaccgc gcgagggcgc
     2941 ccgacgaagg cgcgcacgtc tttcactccc gaggcgagct gcacgtcctg cagcatcgcg
     3001 gcgttgtcgg ggtcgggcgt gacgacgctg agcgtcgccg tttgcgtgtc gtagagcacg
     3061 gggaagacgc agtcgcgctc ggcgagcttc ttcggcacct tgtcgagcgt ctgccgattg
     3121 atctcggctt tggcgagctt ctccgtggtg acgaaacgcg tgcgatgcag cgacgcgagg
     3181 aacttgagga gctgggcctc ctcgagcgcc ttcatctcga gcaggatctc ttcgatacgg
     3241 tcgccgctgc gggcggccag gttcagcgcc gcttcttgtt gctcgggcgt gaccagcttg
     3301 tccgccacaa gccgttcgag aaggcgcata tggccagcag gtggcatgat gggttgagcc
     3361 cgctagtata tcggggcctc gcggctccgg ctcctttcgc ggcgaaacgc cggcagacaa
     3421 aatccggcca agcgacccga cattcgaaat tccgccgaaa tcgcgggttt gtcgggtgta
     3481 ggcgcggagt cgcgtgcgga aaaacaagct gtccttgacg tgcgcctcgg cgaacgctag
     3541 gaatgcgccc cttccgtccc caaggtccca ggaaatatgc cgacttacgt ggcaaagccc
     3601 gccgaagccc aagcaacccg caagtggtac gtcgtcgacg cgaccgatca accgctgggg
     3661 cgcttggcct ccaagatcgc cgccgtcctc cgcggtaagc acaagcctac ctacaccccg
     3721 cacgtcgaca ccggggactt cgtcatcgtc gtgaacgcgg cgaaggtgaa gcttacgggc
     3781 aggaagacga ccgacaagat gtactaccgc cacagcggct accccggcgc catccgtggg
     3841 gagagcttcg agcacgtcgt gcagcgtcat cccgacgcgc cgatcacgct cgccgtgaag
     3901 ggcatgcttc cgaagaacgt cctcggccga cagatgctcg agaagctgaa ggtctacggc
     3961 aacggggatc atccgcaccg ggcgcaaaag cccgaatcgc tcaacgtctg agagcccgag
     4021 gtaatcaagg accatgtccg aagcgcaatc gacttacgcc actgggaaac gcaagaccgc
     4081 cgtcgcgcgc gtctggattc agccgggcag cggcgccgtc accgtcaacg gcgccagcat
     4141 cgacaactac ttcgagcgtg agacctcgcg catggtcgtg cgtcagggtc tcgagcttct
     4201 cgagctccac gacaagtacg acgtcgtcgc gagcgtctgc ggcggtggcc acagcgccca
     4261 ggccgaggcc atgcgccacg gcatcgcgcg cgccctcacc tcggaagatc ccgagcgtcg
     4321 tcgcgccttg aagcgcgcgg gttacctcac ccgtgacgac cgcaagaagg aacgcaagaa
     4381 gtacggtcag gcaggcgccc gcaagcgctt ccagtactcc aagcgctgag ccatgctgaa
     4441 agtcggcatc atcggcgcag gaggttacac cggcgccgag ctggtgcgac tcacgtatgc
     4501 tcatccagag ctcgaactcg agctcgttgc ggcacgcgaa aaagcgggca agcgcctggg
     4561 cgaagtgttg cccagcgtgc tgggcgtcaa agcgctcgaa gaccgcttgc tcgagacgtt
     4621 cgatcccgag caagccgtcg cgctcaaatc gcgcatcgac gtcgcgttcc tcgctttgcc
     4681 tcacggcgcg agcgcccgcg ccggcaaggc gttgttcgac gctggcattc aggtcgtcga
     4741 cctttcggcg gattttcgcc tcaaagatgc cgaggcctat cggcgaacgt acgccgagca
     4801 tccggcgccg gagttgctgc gcacggcggt gtacggacaa ccggagctcc accgagacga
     4861 gctgcgcgac gccaagctca tcgccgcgcc cggttgccat gtgacgacgg cgctgttgcc
     4921 gctcgctccg ttgctgaaag caaagctcat cgatccgaac ggcatcgtga tcgattcgaa
//
LOCUS       DASTKS010000465         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1412950, whole genome shotgun sequence.
ACCESSION   DASTKS010000465 DASTKS010000000
VERSION     DASTKS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412950"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFQ35_09720"
     CDS             complement(<1..816)
                     /locus_tag="VFQ35_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930955.1"
                     /translation="MAALTQSARAVALEPVSDVPATSSDVAVRHTRIIEVPLPNKLPP
                     DPEALALSTKIDSIIYEVVQDLGLNVDISERTPRRESDEDLLEAAKEGWVVAPKLERN
                     RGHWLLRIVAAPPGSRVLYSANVVVEPDSLEKRIVLAMRDLMEAARGAKPSPSAAPPP
                     PPPSTQPLAAHSPGRAILAVNAALLGGFAGYSLQRASGSTDDRLTYPLAALGAGVGLG
                     SAMLVAEEWDIEVGDAWFLSAGMLWPTLSASFIARGYDVSRANQYGYGLLGATA"
     gene            complement(896..2344)
                     /locus_tag="VFQ35_09725"
     CDS             complement(896..2344)
                     /locus_tag="VFQ35_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930956.1"
                     /translation="MSHADEPRRVGEPNVLREPGEITQVVDAFDGDDPFDLHLTLGYE
                     TSWKSASIRRETSIMQPGLTTGNYTSSALNVAEYKESTSRLLTRADVGLYHDVALVVR
                     MPIILANERELSGLDGSEAIQSVALQGAPGEQLFRLPFKSPKRSGIEYLAVGIDAAIM
                     NQARDRTKPTWVVGIEGRFNISEPMHACNASSSAVNLSGTQVKCANTSDVNRNGKADG
                     GAEGSFSSGDRPSGVSRGVTGLELHSYVSRRVKYVEPYGGFRALFEFQNASSDYGQVD
                     LKGSLVNHPPLRGTMLMGVQVIPWEIRDRFQRMTMDFRFTGTYVSEGRDYSELFDALG
                     SSDAPTIREPNYSEYMGGPTPNSSVVNPSSRRVYFTGLTDVQAHGSYTLSTSFTWQAG
                     EFVKFNAGAAYTLTQGHFITYDQACNPDFTGDIARSGPCHKESGSAENPTVSASGIPN
                     PSFRKTLNDPGNRFRVDDVRQIDAWINATVMF"
BASE COUNT          400 a          793 c          876 g          429 t
ORIGIN      
        1 cgccgtcgcg ccgagcaggc cgtagccgta ctggtttgcg cggctcacgt cgtagccgcg
       61 cgcgatgaac gacgccgaga gagtcggcca gagcatgccc gccgacaaga accaagcgtc
      121 gccgacctct atgtcccact cttcggcgac gagcatggcg ctgccgagcc cgacgccggc
      181 gccgagcgcg gcgagggggt aggtgaggcg atcgtcggtg gagccgctgg cgcgctggag
      241 tgagtacccc gcgaagccac cgagcaaggc cgcgttcacc gccaagatcg cgcgcccggg
      301 tgaatgcgcg gccaggggct gagtgctcgg cgggggaggc ggcggggctg cgctcggcga
      361 cggcttcgcg ccgcgcgcgg cttccatcaa atcgcgcatc gcgagcacga tgcgcttctc
      421 caacgaatcc ggttcgacga cgacgttcgc ggagtagagc acgcgcgaac cgggcggcgc
      481 ggcgacgata cgcagcagcc aatggccgcg gttccgctcg agtttcggcg cgaccaccca
      541 gccttctttg gccgcctcga gcaggtcctc gtcgctttcg cggcgcgggg tgcgctccga
      601 gatatcgacg ttgagcccca gatcttggac gacctcgtag atgatggagt cgatcttcgt
      661 gctgagggcg agcgcctcgg gatcgggcgg cagcttgtta gggagcggca cttcgatgat
      721 gcgggtgtgc cttacggcga catcgctgct cgttgcgggc acgtcgctca ctggctcgag
      781 agcgaccgcg cgagccgatt gcgtgagcgc ggccacgctc agcagagccg ccagcgagca
      841 tcgcgcgagg acgcgggctc gggtcagtgc tgaagcgaag cgggccacgg cctgatcaga
      901 acatcaccgt ggcgttgatc caagcgtcaa tctgcctcac atcgtcgact cggaagcggt
      961 ttccgggatc gttcagcgtt ttgcggaagc ttgggttcgg aatgccgctc gcgctcacgg
     1021 tggggttctc ggcgctgccg gactctttgt ggcagggccc ggagcgcgcg atgtcaccgg
     1081 tgaaatctgg gttgcaggct tggtcgtagg tgatgaaatg gccttgcgtc agcgtgtagg
     1141 cggcgccggc gttgaacttc acgaactcac cggcttgcca cgtgaaggag gtgctcaggg
     1201 tgtagctgcc gtgcgcttga acgtcggtga ggcccgtgaa atagacgcgc cgcgagctcg
     1261 gattgacgac cgacgagttc ggcgtgggcc cacccatgta ctcgctgtaa ttgggctctc
     1321 ggatcgtcgg cgcatcgctc gacccgagcg cgtcgaacag ctccgagtag tcccgcccct
     1381 cggagacgta ggtcccggtg aaacggaaat ccatcgtcat gcgctggaag cgatcgcgga
     1441 tctcccaggg gatgacctgc acgcccatca gcatcgtccc gcgcagcggc ggatggttca
     1501 cgagtgagcc cttcagatcg acttggccgt agtcgctgct cgcgttttga aactcgaaca
     1561 gcgcccggaa gccgccgtag ggttcgacgt acttgacccg gcgcgagacg tagctgtgga
     1621 gctcgaggcc ggtgacgccg cggctcacgc cgctcggtcg gtcgccggag ctgaagctgc
     1681 cttcggcgcc gccgtcggct ttgccgttgc ggttcacgtc cgaggtgttc gcgcacttga
     1741 cttgggtgcc gctcaggttc accgccgagc tactcgcgtt gcaggcgtgc atcggctcgg
     1801 agatgttgaa gcggccctcg ataccgacga cccaggtcgg cttcgtgcga tcgcgcgctt
     1861 ggttcatgat cgcggcgtcg atgccgacgg cgaggtactc gatgccgctg cgcttcggcg
     1921 acttgaacgg caagcggaac agctgctcgc ccggcgcgcc ttgcagagcg accgactgaa
     1981 tcgcctcgct accgtcgagc cccgagagct cgcgctcgtt cgcgaggatg atgggcatgc
     2041 gcacgacgag cgcgacgtcg tgatagaggc ccacgtccgc gcgagtgagc aggcggctcg
     2101 tgctctcctt gtactcggcg acgttcagcg ccgaggacgt gtagttcccc gtcgtgagcc
     2161 ccggctgcat gatcgaggtc tcgcgccgaa tcgacgcgga cttccagctc gtctcgtagc
     2221 cgagggtgag gtggagatcg aaggggtcgt cgccgtcgaa agcgtcgacg acctgcgtga
     2281 tctcacctgg ctcacgcaag acgttgggtt cgccaacccg ccgtggctcg tcggcgtgtg
     2341 acaccggcga gcacagccac agggccgttg ctatcgcaac cccgacggct cggcgctctt
     2401 ggcacgaagg aaggctcgga agcgcaaatc ggcggaccat tcagcgttgc ccgcccgcga
     2461 cgctagcggc gcgaggcgga aactgtcaac ggaccatc
//
LOCUS       DASTKS010000466         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1635178, whole genome shotgun sequence.
ACCESSION   DASTKS010000466 DASTKS010000000
VERSION     DASTKS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635178"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2513
                     /locus_tag="VFQ35_09730"
     CDS             <1..2513
                     /locus_tag="VFQ35_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930957.1"
                     /translation="LASASIAGGNSESNSSQTNGGTGGTGGHSGAGGLGGTGHHGGSG
                     SLAAGAAGVGDEAGAGGMGIAPANGGETSAGGETSLGGTTSMGGVASTGGLASTGGVT
                     AAGGAAGAPAEGGIGGESGGGGASGGAVPETPLHCDDHNACTHDSVADGACKHEAEPN
                     GTPCDDQNFCTFGDECRDGVCTSGTPQASRASVLGTLESYGTGSVQSFGDDRLFFVDR
                     ALFAESRLSVGKVENGVVHRLASLDVNGLPSTWISTAWGGLAAVTEGSTSVLIGGDPR
                     LITLFSTDESGALTRRGAVALMDGASPAMTSMLGRGNKLLVCANFGFFSAPIGTVYVI
                     DVTDADSPVVVTHGAVNGSCGSLALSEDGQRLYANTANGVQFMDFSKWDGSATFTFDN
                     QLFVPENSGLSLKGSRLLVRSGDLVRVFDESSRALLSSFNVAGARSSAWTDAGVFTQG
                     IRWDGKQTEVFVALHDTAGTLIDERTIARLDYQADLTTRAVAAPELAVSPYGGPIWKV
                     SSRGFQPLQQPEMAVLDSVHAQGNILHTRGRTLWGRVDVSDPTRPAWQGSGPHQNPHL
                     SFKLDHSLSPAALVSEFDAALFDGATRRLVVQSGYGHSTTLSVEGVSADENEHSIPDQ
                     SFTLPGGIASLASFGDSIYRAVSTTTSATFQRFRIVDLEAGIAAPTFELTFTQPTSVQ
                     TFDVDPVAQRALFAFGGYDSNSATFGKLVVIDLSTDPPSTIESADIHELPYDALLRGD
                     HLVYTDGTTLAFRQVLGSEERRITIESPGVDQLLAFDGNVVYFSARKRLAAASRASET
                     LLLDVPTRNTMLSLVETPKALVAAGGNQLITLSPACQP"
     gene            complement(2580..>2843)
                     /locus_tag="VFQ35_09735"
     CDS             complement(2580..>2843)
                     /locus_tag="VFQ35_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET9930958.1"
                     /translation="IGRKIRSVAQHRQVLCITHLPQIAVFAEQHFKVEKAVQGERTVS
                     TVRRLAPKEQREEIARMLGGLNITAKTRAAARELLLHARSSAA"
BASE COUNT          488 a          960 c          914 g          481 t
ORIGIN      
        1 cgttggcgtc cgcgtcaatc gctggtggca acagcgagtc gaacagctcg cagacgaatg
       61 gcggtacggg ggggaccggc ggacactccg gagcgggtgg gctcggcggc accgggcatc
      121 acggtggctc gggatcgctc gcggcgggag cggcgggcgt gggggacgag gccggcgcgg
      181 gaggcatggg catcgcgccc gcaaacggtg gcgagacgtc ggcaggcggc gagacgtcgc
      241 ttggcggaac gacatccatg ggaggagtgg cgtcgacggg cgggctcgcg tcgacgggcg
      301 gagtgaccgc ggcaggcgga gcagcaggcg ctcccgctga agggggaatc ggtggcgaaa
      361 gcgggggcgg cggagcaagc ggcggagccg tgcccgagac acctctgcat tgtgacgacc
      421 acaacgcctg cacccacgac agcgttgccg acggcgcctg caaacacgag gcggagccga
      481 acggtacgcc ttgcgacgat caaaacttct gcacgttcgg cgatgagtgt cgggacggag
      541 tgtgtacatc cggaacgccg caggcgtccc gcgcgtccgt gctgggcacg ctcgagagct
      601 acggcaccgg cagcgtccaa agtttcggcg acgaccgtct gttcttcgtg gatcgcgcct
      661 tgttcgcgga gtcgcgcctg agcgtgggaa aggtcgagaa cggtgtggtc caccgcctcg
      721 cgagcctcga cgtgaacggc ctgccgagca cctggatatc gacggcctgg ggaggcttgg
      781 ccgccgtcac cgagggttcg accagtgtct tgataggcgg cgaccccagg ctgatcacgc
      841 tgttctcgac cgacgagagc ggagcgctga ctcgacgtgg cgccgtcgcg ctcatggacg
      901 gagcttcgcc ggcgatgacc agcatgctcg gccgcggcaa taagctgctc gtgtgcgcga
      961 acttcggttt cttcagcgcg ccgatcggca cggtgtacgt gatcgacgtg actgacgccg
     1021 atagtccggt cgtggttacg cacggcgccg tcaacggctc gtgcggctcc ttggcgctga
     1081 gtgaagacgg tcaacgcctc tacgcgaaca ccgcaaacgg cgttcagttc atggacttct
     1141 ccaagtggga cggcagcgcg accttcacct tcgacaatca gctgttcgtt cccgagaact
     1201 ctggtctctc gctcaagggc agccggctct tggtgcgcag cggcgatctc gtgcgcgtct
     1261 tcgatgaaag ctcgcgtgcg ctgctgagct cgttcaatgt ggcgggtgcg cggtccagcg
     1321 cttggaccga tgcgggcgtc ttcactcagg gaattcgttg ggacggcaag caaacggagg
     1381 tgttcgtcgc actccatgac accgcgggca cgctcatcga cgagcgcact atcgcgcgct
     1441 tggactacca ggcagatctg acgacccgcg ccgtggcggc gcccgagctc gcggtgagtc
     1501 cctacggcgg cccaatttgg aaggtttcga gtcgcggctt tcagcccttg caacagccag
     1561 aaatggccgt gctggactcg gtccacgcgc agggcaacat cttgcacacg cgaggccgca
     1621 ccctctgggg tcgcgtcgat gtgtccgacc caactcgccc cgcctggcaa gggagcggcc
     1681 cgcatcaaaa tcctcacctg agcttcaagc tcgaccactc actctcgccc gccgcgctcg
     1741 tgagcgagtt cgacgcagcg ctgttcgacg gtgcgacccg ccgcctcgtc gtgcagtcag
     1801 gctacggcca ttcgacgacg ctgagcgtcg agggcgtcag cgccgacgaa aacgagcact
     1861 ccatcccaga tcaatcattc actctgcctg gaggcatcgc cagcttggcg agctttggcg
     1921 actcgattta tcgcgccgtc tccacgacga cgagcgctac atttcaacgc tttcgaatcg
     1981 tcgatctcga agccggcatc gccgcaccga ccttcgagct cactttcaca cagccaacct
     2041 cggtacagac cttcgacgtc gatcccgtcg cgcaacgcgc cttgttcgcg ttcggcggct
     2101 acgactcgaa cagcgcaact ttcggcaaac tcgtcgtcat cgacctctcc accgacccac
     2161 cgtcgacgat cgagtcagcc gacatccacg aactcccgta cgacgccctc ctccgcggcg
     2221 accacctcgt ctacaccgac ggcaccaccc tcgccttccg gcaagtgctc ggcagtgaag
     2281 agcgcaggat caccatcgag tcgccgggtg tcgaccagct cctcgccttc gacggcaacg
     2341 tcgtctactt cagcgctcgc aaacgcctcg ccgccgcctc acgcgccagc gaaacgctgc
     2401 tcctcgacgt gcccacccgc aacaccatgc tctccctcgt agaaaccccg aaagccctcg
     2461 tagcggccgg gggcaaccag ctcatcaccc tatcacccgc ctgtcagcca tagagccacc
     2521 tccgaacgcg ctcgagttcg agctgccagg gttgatgggc ggccaccgcg cgcggcagcc
     2581 taagccgccg agctcctcgc gtgcagcaag agctcgcgtg ccgcggcgcg agtttttgcc
     2641 gtgatgttca gcccgccgag catgcgcgca atctcttcgc gttgctcctt cggcgcgagt
     2701 cgacgaacgg tgctgaccgt gcgctcgcct tgcacggctt tctcgacctt gaagtgttgc
     2761 tcggcgaaca cggcgatttg cggcaggtgc gtgatgcaca gcacttgccg gtgctgggcc
     2821 acgctccgaa tctttcgccc gat
//
LOCUS       DASTKS010000467         8529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1413474, whole genome shotgun sequence.
ACCESSION   DASTKS010000467 DASTKS010000000
VERSION     DASTKS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413474"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..2727
                     /locus_tag="VFQ35_09740"
     CDS             223..2727
                     /locus_tag="VFQ35_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9930959.1"
                     /translation="MKSGRVEPELHALRQSAEELSRRGRETLTSAHLLAAIVKAGGPA
                     ADLLEERRFGLEETLRAARTTVDEVADPIRAALARAHDIAARMGFTQASAVHLLVALL
                     NEPRSAARRALEQAGVDVSRLRTTAMQLGLGLMPGRRAPATRPILAMPAPEVPRAPAS
                     ARSEAPKSTPPRSRSAVSIPIVPKARVALPKESAPPTASESTPAPAAAAAATTPPTVP
                     KEAAKAPTPAVASSSRFELDPQRFPTLSMLGKNLTLRAARGELDPVVGRDAVIEQALD
                     VLAKREGNNPCLVGVAGVGKTSVVRGLAQRIAAQRDVASLDDKIIIEIPVGELLAGTG
                     VRGALAARLGAMRKEVAASMGRIVLFFDEIHQLFSGEAAEEIGSELKLALARGELPCI
                     GATTLEEYQKAIGQDAALARRFSLVEIDEPSREDAYLVLEALRPRLEAHHRVRYADEA
                     LALGVAWSVRYLPGRALPDKAVSILDLAGARTRRRGKPEVIADAVAEVVAELADMPIE
                     RLLESDGDRMLQLEEILAERVVGHGPQIRRIARILRRNAAGLGSRRPIGTFLLLGPSG
                     VGKTETAKAIAEILFHCDSAMTRIDLSEYSEAHAVARLIGAPPGYVGHEAGGQLTEAV
                     RRRPYQVILLDELEKAHPDVLTSFLGLFDEGRLTDGRGRTVDFTNTVILMTSNIGSSE
                     VTRVERRVGFGSTESQSSDLDTRVVARAREKLAPELYNRIDEVLVYSPLSRDEVREIA
                     RRLLGSVSRTLAAQRGLRLEFSAEVIEHLLDHGGFEPALGARPMKRSIARLIEAPLAE
                     KILAGELPRGAVVLVALEQGELDFDVLESDGTQAAE"
     gene            2915..4654
                     /locus_tag="VFQ35_09745"
     CDS             2915..4654
                     /locus_tag="VFQ35_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930960.1"
                     /translation="MRRKEAGGASADPGVLVKCDGAASGWDQALPPTTGLMNRVVMSI
                     SSVSRTNGRFGSTFAVACVAIALLGSGCGNAARDGHESSNGGARGDTGGAGGVAPNGG
                     RAGATAGATASGQGGSMGGSPSTSGASGALDAAGASGEVAGSGGAQSASGGTGGVGGD
                     SNAAGNAGRGGDTGGCVEGVPCQCAELVGTTQCQSGVAACSCPPAEQCRTPSGAPCFE
                     PCGGDPFGGWVMVDSCFRTSHAGVGTACERLVQAMPNGSDLRLRILDGGRYDLGGNER
                     WSIQLSASLSCLLLDSVQKCSERSIGVNPFMFSSGERLPCKPNACGVCECAGDVGAAY
                     SQESGSWTRQGNAIRLGNNQYPYCVKGDELWIGGGFSEDSPIGAYKFKRQSCQGTPMI
                     SCEQLGRTECARTSGCRMGSCEPLPATSADCAAKSEIDCNQSAVCNWNYETCSGKASC
                     DFANCDSLPGCSWGQPVPRCGGVAQEVCAELDPKTCLVDHGCSIVSCWPGEEDCRRLN
                     ATECAKAPGCKPADPQASVACVGTAQCWKQADQAVCRKLGCESMPLCDGQIDCSSFTV
                     AECHEHAGCRIEW"
     gene            4707..5330
                     /locus_tag="VFQ35_09750"
     CDS             4707..5330
                     /locus_tag="VFQ35_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HET9930961.1"
                     /translation="MLKVEPHPLLELGSVVAHHRAPLGATTTPEWVLELLRPHASTPL
                     SPIDDEVRAKVRDLLRFGGYKPTGRGKPASEYLVRAASEGTLRPINLAVDIANAVSLH
                     SGLPLSVVDLGRARAPLGVGVAAPGTNYVFNASGQSIDVSGLLCLHDAGGAVANAVKD
                     SERTKTRADTEHTLTIIWGARALAERVSRARQWLVELLTRADATIDD"
     gene            complement(5394..6068)
                     /locus_tag="VFQ35_09755"
     CDS             complement(5394..6068)
                     /locus_tag="VFQ35_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930962.1"
                     /translation="MKELGTEAQALVRAGRVAQRPTMADRERNFEALRARIGDAAFAE
                     TASHVALHAGRSLPMTAALTAVGVVVVAGGLFFGLRGRVSSGDTKAAVSVRLPPVVEV
                     VNAPPDSAHQLAQSSEPAPAKVAAPQASTASNKRASTLALEVAILSRAASSLRAGRPT
                     DALKAVDEHQAKFPNGLLTEERRAARAQALCALGRRGEADADLARLSSSSPQAVRARN
                     ACANSR"
     gene            complement(6065..6670)
                     /locus_tag="VFQ35_09760"
     CDS             complement(6065..6670)
                     /locus_tag="VFQ35_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9930963.1"
                     /translation="MPVRNSLAPAQRASEPAEPSASLALPSFAVVYRSYFDFVWSSTR
                     HLGIDRDAIDDVVQEVFIVIHARLHTVQQPESLRSWIYGVVRRTVSRHRRARKVRDNT
                     RADWAELDSAAAATTTMTPLEQHERDDNLALLSSLLNQLDEAKREVFFLAELEEMTAP
                     EIAQALEIPLNTVYSRLRAARLAFDAALARHTAKLTGTRRS"
     gene            7122..7892
                     /locus_tag="VFQ35_09765"
     CDS             7122..7892
                     /locus_tag="VFQ35_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930964.1"
                     /translation="MAACLLFACEPDLNVGSWKCPWPSRIPDGGDSVGLTPYTGPVRV
                     PWSTSFETDMCDYDREQGFCYASPNSSFGFIDAPAHSGRRAMAFSVSLGSTAFEQARC
                     VREGAMPVHGMYTAWFLLPSNVTNSGTWSLVHFRGGDSDGTNLTALWDVDVSNNADNG
                     SSYLYVYDQRRWLSITPPEKVPVPTGKWFKLDVRWRQAADESAEIELLQDGRSVLVIE
                     GASDSFKWRQWYVGNWANSLSPRSNIVYVDDVAIRSLP"
     gene            complement(7953..>8529)
                     /locus_tag="VFQ35_09770"
     CDS             complement(7953..>8529)
                     /locus_tag="VFQ35_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930965.1"
                     /translation="EKRDAGTGGSDANEKPEERRPWRILARPLGTVDFGPLPRPAVGF
                     GLGVGWSSAPWRVLVVGRNVLEQTVFSSSQLQDVQADVHRLMFELWACRTWQANIIEF
                     GPCVVPAVERISARGGGLGVKPAEVNTLGFALGAGVTTEVHVARWFALAANANLSVET
                     KRPRWVLAGLGEVYQLGMVSLSLSLGAELSF"
BASE COUNT         1423 a         2686 c         2972 g         1448 t
ORIGIN      
        1 cgccgtcgcc tgccaccagg ggcagccgag tagcctcgtc gttcgcgaag aggacgagac
       61 cgtcgcttgg accaaccagg cgagcggtga ggtcatggtc ctcgcggggg agctcgaatg
      121 gtgggcgcct tgaacgcgga acgcgtttgg ccgacggttt gggcttcgta aagctgccga
      181 aaacttgccc ggtttcggca gcttgggtga agtctgccca ggatgaagtc cggacgtgtc
      241 gagccagagc tccatgcctt gcgtcagagc gccgaggagc tctcgcggcg ggggcgcgaa
      301 acgctgacga gcgcgcactt gctggcggcc atcgtgaaag cgggagggcc ggcggccgac
      361 ctcttggagg agcgccgctt cgggctcgaa gagacgctgc gcgccgctcg cacgacggtg
      421 gacgaagttg ccgacccgat ccgggcggca ctcgcgcggg cccacgacat cgccgcgcgc
      481 atggggttca ctcaagcatc tgcggtgcat ttgttggtgg cgttgctcaa cgagccgcgg
      541 tcggctgcgc gacgcgcttt ggagcaagct ggggtcgatg tttcgcgtct gcgcacgact
      601 gccatgcagc tcggcctcgg gttgatgccc ggccgacgcg cgcccgcgac gcggcccatc
      661 cttgcgatgc ccgcgcccga ggtgccgcgc gctcccgcgt cggctcggag cgaagcccca
      721 aagagcacgc cgccgcggag tcgttcggcc gtgagcatcc caatcgtacc gaaggcacgt
      781 gtggctctcc cgaaggagtc ggcgcccccg accgcaagcg aatccacgcc ggcgcccgcc
      841 gctgccgccg ctgcgaccac gccgcccacc gttccgaagg aagcggcgaa agctccgacg
      901 cccgctgtcg cgtctagttc gcgcttcgag ctcgacccgc agcggttccc gaccctgtcg
      961 atgctcggaa agaacctcac gctgcgcgcc gcgcgcggtg agctcgaccc ggtcgtgggc
     1021 cgcgacgcgg tgatcgagca agccctggac gtgctcgcca agcgcgaagg caataaccct
     1081 tgcctggtcg gagtcgctgg cgtgggcaag acgagcgtcg tgcgtggcct cgcgcagcgc
     1141 atcgccgctc agcgcgacgt ggcttcgctc gacgacaaga tcatcatcga gatcccggtt
     1201 ggagagctgc tcgccggaac cggtgtccgc ggtgcgctcg cggcccggct cggcgcgatg
     1261 cgcaaggaag tggccgcctc gatggggcgc atcgtcttgt tcttcgacga gatccatcag
     1321 ttgttcagcg gtgaagcggc ggaggagatc ggcagcgagc tgaagcttgc gctcgcgcgc
     1381 ggagagctcc cttgcatcgg tgcgacgacc ctcgaggagt atcaaaaggc catcggacaa
     1441 gacgcggcac tcgcgcggcg tttcagcctg gtcgagatcg acgagccgag tcgggaagat
     1501 gcgtacctgg tgctcgaggc gctgcgaccg cggctcgagg cgcaccaccg cgtgcgctac
     1561 gcggacgagg cgctggcgct cggcgttgcc tggtccgttc gctatctgcc gggacgggcg
     1621 ctgccggaca aggcggtttc gatcctggac ttggcgggtg cgcgcactcg ccgccgtggc
     1681 aagcccgagg tgatcgccga cgcggtagcc gaggtcgtgg ccgagctcgc cgacatgccg
     1741 atcgaacgcc tgctcgaatc ggacggggac cgcatgctgc agctcgaaga aattctggcg
     1801 gagcgcgtcg taggtcacgg cccgcagatt cgtcgcatcg cgcgcatctt gcggcgcaac
     1861 gcggcgggtc tcggctcgcg ccgccccatc ggaacgttcc tcttgctcgg cccgagcgga
     1921 gttggcaaaa ccgaaacggc caaggccatc gccgagatct tgtttcactg cgacagcgcg
     1981 atgacgcgca tcgacttgtc cgagtactct gaagcgcacg ccgtcgctcg gctcatcggg
     2041 gcgccgcccg gctacgttgg gcacgaagcg ggcgggcaac tgaccgaagc ggtgcgtcgg
     2101 cgcccctatc aggtgatctt gctcgacgaa ctcgagaagg cacatcccga tgtgctcacg
     2161 tccttccttg ggctgttcga cgagggacgc ctcaccgatg gccgcggtcg caccgtcgat
     2221 ttcacgaaca cggtgatctt gatgacgtcg aacatcggct cgtccgaggt gacgcgcgtc
     2281 gagcgccggg tcgggtttgg aagcaccgag tcgcaaagct ccgaccttga cacgcgcgtc
     2341 gtggcgaggg cgagagagaa gcttgcgccg gagctctaca atcgcatcga cgaggtgctc
     2401 gtatactcgc ccttgtcgcg cgacgaggtg cgagagatcg cccgtcgcct gctcggttcc
     2461 gtctcgcgga ccctcgccgc gcagcgcgga ctgcggctcg agttcagcgc cgaagtgatc
     2521 gagcacctgc tcgatcacgg cgggttcgag cctgcgctcg gcgctcggcc gatgaagcgc
     2581 tcgatcgcgc gcctcatcga agcacccttg gcagaaaaga tcctcgccgg tgaactaccg
     2641 cgcggagcgg tggtgctcgt cgctctcgag caaggggagc tcgacttcga cgttctcgag
     2701 agcgacggga cgcaagccgc agagtagata cgctcctcga gcggcgccgt tgcgcgcccg
     2761 gtgatcccgc tcggcgcgac cgggcatgtc aggcatatgc gagaggagcg gcacgagctg
     2821 ggtgtgaccg aatcggtggg gccgcagcgc aaccggagcg ccgcggtaat agccgcaata
     2881 ttccgctgtt gcgtgaagag tggggcggct gctgatgcgt cgtaaggaag cgggcggcgc
     2941 gtcggcggac ccgggcgtgt tggtgaaatg tgacggggcg gccagcggct gggatcaagc
     3001 cctgccgcca acaacggggc tgatgaatcg agtggtgatg tcgatctcgt cggtttcgag
     3061 aacgaacggc aggttcggtt cgacgtttgc agtcgcgtgc gttgcgatcg ccttgctggg
     3121 ctcgggttgc ggtaacgcag ccagggacgg gcacgagagc agcaacggcg gggcgcgagg
     3181 cgacacgggt ggggcggggg gagtcgcgcc gaacggcggt cgcgccggtg caaccgctgg
     3241 cgctacggcg agtggtcagg gcgggtcgat gggcggctct ccttcgacta gcggcgcgag
     3301 cggagcgctc gacgccgcgg gcgcatcggg cgaagtcgcc ggtagcggcg gcgcgcaaag
     3361 cgcgtccggc ggaacgggcg gcgtcggcgg cgatagcaac gcggcgggca atgccggacg
     3421 aggcggggac acgggaggct gcgtcgaagg cgtgccgtgt cagtgcgccg agctcgttgg
     3481 tacgactcag tgtcagagcg gtgttgccgc gtgctcctgc ccgcccgcgg agcagtgccg
     3541 tacgccttcg ggagcgccct gcttcgagcc gtgcggggga gatcccttcg ggggctgggt
     3601 gatggtcgac tcttgcttcc ggacttcgca cgccggcgta ggaaccgcgt gcgagcgcct
     3661 ggtccaagcg atgccgaacg ggagtgattt gcgactccgc atcttggatg gcggtaggta
     3721 cgacttgggt ggcaacgagc gatggtccat ccagctttcg gcgtcgttgt cgtgccttct
     3781 cctcgactcg gtacaaaagt gcagcgaacg gtcgatcggc gtaaacccgt tcatgttctc
     3841 gtccggggag cgcctgccat gcaagccgaa tgcgtgcggc gtctgtgaat gtgcgggcga
     3901 cgtcggggcc gcttactctc aggagagcgg gtcgtggacc cgtcaaggca acgcgattcg
     3961 gctcggcaac aatcagtatc cgtattgcgt caaaggcgac gagctctgga ttgggggcgg
     4021 gttcagcgaa gactcgccaa taggtgccta caagttcaag cggcagagct gtcaagggac
     4081 gcccatgatc agctgcgagc agctcggaag aaccgagtgc gcgaggacga gcgggtgcag
     4141 aatgggctct tgcgagccgt tgcccgcgac ttccgcggac tgcgcggcga agagcgaaat
     4201 cgattgcaac cagtccgcgg tctgcaattg gaattacgaa acgtgctcag ggaaggcttc
     4261 gtgcgacttt gcgaactgcg actcgctccc cggctgttcc tgggggcagc ccgtcccgcg
     4321 ctgcggtgga gttgcgcaag aggtctgcgc cgaactcgac ccgaagacgt gcctggtcga
     4381 ccacggctgc tcgatcgtct cgtgctggcc gggagaagaa gattgccggc ggctgaacgc
     4441 gacagagtgc gcgaaagcgc caggctgcaa acccgcagat cctcaggcga gcgttgcgtg
     4501 cgtggggacc gcccagtgct ggaaacaggc cgatcaggcc gtgtgccgca agctcggttg
     4561 tgagtcgatg cccctgtgcg acgggcagat cgactgctcg agcttcacgg tcgcggaatg
     4621 tcacgagcac gccggctgcc gcatcgaatg gtagcggagc ggctcgggtt tgtacggagc
     4681 gtgcgccgcc gatatagtgc gtcgcgatgc tcaaggtcga acctcatcct ttgctcgagc
     4741 tcgggagcgt cgtcgctcat catcgcgcgc cgctgggcgc gacgacgact ccggaatggg
     4801 tgctcgagct cttgcgaccg cacgcgtcta cgccgctttc accgatcgac gacgaggtgc
     4861 gagccaaggt gcgcgacctg cttcgcttcg gcggctacaa acctaccggg cgcggcaagc
     4921 cagcttctga atatctcgtg cgggctgcaa gcgaaggcac cctgcggccg atcaacctcg
     4981 cggtcgacat cgcgaacgcg gtgtcgctcc acagtgggct gcctctcagc gtggtggact
     5041 tgggccgcgc ccgtgcgccg ctcggcgtcg gagtagccgc gcccggaacg aactacgtgt
     5101 tcaacgcttc cgggcagtca atcgacgtga gcgggctctt gtgccttcac gacgccggcg
     5161 gtgccgtggc gaacgcggtg aaggactcgg agcgcacgaa gactcgagcg gacaccgagc
     5221 acaccctgac catcatttgg ggcgcaaggg cgctcgcgga acgcgtatct cgagcacgac
     5281 agtggctcgt cgagctactc acgcgagcgg acgcgacgat cgacgactag cgcgacgacc
     5341 acgagtggcc cgagcccctg acgtgatgcc gacggacgcg agcaaacggc gcatcagcgc
     5401 gagttggcgc aggcgttgcg tgctcgcacg gcttgtggcg aagacgagga gagccgggcc
     5461 aaatccgcat cggcctcgcc gcgtcggccg agcgcgcaca gggcttgagc ccgtgccgcg
     5521 cgtcgctctt ccgtcagcaa accattgggg aacttggctt gatgctcgtc tactgctttg
     5581 agcgcgtcgg tagggcgtcc cgcgcgcaag ctgctcgcgg cgcgcgatag gatcgcaacc
     5641 tccagagcca acgtgctggc gcgtttattc gaggcggtgc tcgcctgcgg cgcggccacc
     5701 ttggcgggcg caggttcgct gctttgagcc agttggtgcg ccgagtcggg cggggcgttg
     5761 acgacctcca cgacgggggg caagcgcacg ctcacggccg ccttcgtgtc gccagaggag
     5821 acgcgcccgc gcaaaccgaa gaacagccct cccgcgacca cgactacgcc gacggcggtc
     5881 agcgctgcgg tcatggggag cgagcggccc gcgtggagcg ctacatgcga cgccgtctcg
     5941 gcgaaagcag cgtcaccaat gcgtgcccgc agcgcttcga aattccgctc gcggtccgcc
     6001 atggtcgggc gctgcgcaac gcgccccgct cgaacgagcg cttgagcctc cgtcccgagc
     6061 tctttcatga gcgcctcgtt ccggtgagct ttgcggtgtg gcgtgcaagc gccgcatcga
     6121 aagcgagccg cgcggcacgc agtcgcgagt aaaccgtgtt gagcggtatc tcgagggcct
     6181 gcgctatctc gggggcagtc atctcttcga gctccgccaa gaaaaagact tcacgctttg
     6241 cttcgtcgag ttggttgagc agactcgaga gcagagcaag gttgtcgtcg cgctcgtgct
     6301 gttcgagcgg tgtcatcgtc gtcgtcgcgg cggcggcgct gtccagctcc gcccaatcag
     6361 cgcgcgtgtt gtcgcgcacc ttgcgcgccc gccgatgtcg gctcaccgtg cgcctgacca
     6421 cgccgtagat ccagctccgc agcgattcag gctgctggac ggtgtggagt ctcgcgtgga
     6481 tcacgatgaa cacctcttgc acgacatcgt cgattgcatc tcggtcaatg cccagatgcc
     6541 gcgtcgatga ccaaacaaaa tcaaaataac ttcgatagac gacggcaaag gacggcaaag
     6601 cgagcgaggc gctcggctcg gcaggctcag acgcccgctg tgcgggcgcc agcgaattcc
     6661 ttacgggcat cgtccgtcgg taaggcagtg tcctcgaggc gcagtctccg tcaacaaatc
     6721 caatcagtct ggttccgctg gccaaaaacg ctaattattc cgcgggtcgg ctgtgttcca
     6781 aaatggacgt cctgcgagcg gccgggggat ctcgcgatga cactaggctg ggaaacggct
     6841 ccgaccgggc ggtggcccga gacgcgctgc ggcgtgctgg tggctcatgc ctggctcgga
     6901 tattcgcctc gggaggtgcc gaaacggctc gagtcagacg gctcggttcg agcatttttg
     6961 cagcgagtgt cgagcggcgc ccgaacgtgc tctcgtggtc gcgcatcact ttgaacgcgg
     7021 cgatttgatg gaacgcaggt cggctgacgc tagactcggg ctgcgtgtca gcttcgaaac
     7081 gccgagcact gcatgcatcg agttcgcgct ttggcgcggc gatggcagct tgccttctgt
     7141 tcgcttgtga acccgatctc aacgtcggaa gttggaagtg tccttggccg tcgcgaattc
     7201 ccgatggtgg tgactcggtt gggctgacgc cctacacggg cccggtgcgc gtgccctggt
     7261 cgacgagctt cgagaccgac atgtgcgatt acgatcgcga gcagggcttt tgctacgcga
     7321 gcccgaattc gagcttcggt ttcatcgacg cgcccgcgca ttcagggcgg cgcgcgatgg
     7381 cgttctcggt gagcctcggg agcacggcgt tcgagcaagc gcgttgcgtg agagaggggg
     7441 cgatgcccgt tcacgggatg tataccgctt ggttcctgct cccttcgaac gtgacgaact
     7501 ctgggacgtg gagcttggtg cacttccgcg gcggcgatag cgacgggacg aacctgaccg
     7561 cgctttggga cgtggacgta tccaacaatg ccgacaacgg cagttcctac ttgtacgtct
     7621 acgatcaacg ccggtggcta tccataacgc ctcccgagaa ggtccccgtc ccgaccggca
     7681 agtggttcaa gctcgatgtg cgttggcgcc aggccgccga cgagagcgcc gagatcgagc
     7741 tgctccaaga cggccgcagc gtgctcgtga tcgaaggcgc gagcgactcc ttcaagtgga
     7801 ggcagtggta cgtggggaac tgggcgaact cgctctcgcc gcgctccaac atcgtttacg
     7861 tcgacgacgt ggcgattcgt tcgttgccct gagttcgggg tcgcgccgcc caaggcgatt
     7921 cgttcgttgc cctgagttcg gggtcgcgcc gctcaaaacg agagctcggc gccgaggctc
     7981 aacgacaacg ataccatccc gagctggtac acctcgccga gacccgcgag cacccagcgc
     8041 gggcgcttcg tttcgacgga taggtttgcg ttggcggcga gcgcgaacca gcgagccacg
     8101 tggacttcgg tagtcacgcc ggctccgagc gcgaatccga gagtgttgac ttccgctggc
     8161 tttaccccga ggccgccgcc gcgcgcggag atgcgttcca cggccggcac gacgcacggt
     8221 ccgaattcga tgatgttcgc ttgccacgtg cgacaagccc aaagctcgaa catcaggcga
     8281 tgaacgtccg cctgaacgtc ctgcagctgg ctcgaagaaa aaactgtctg ctcgagcacg
     8341 ttgcgcccaa cgacgagcac tctccaagga gcagagctcc acccgactcc aagcccaaac
     8401 ccaactgcgg gccgtggtag tgggccgaag tccaccgttc cgagcgggcg cgccaagatt
     8461 cgccaagggc gacgctcttc gggcttttcg ttcgcatcgg agccgcccgt tccggcgtcg
     8521 cgcttttcc
//
LOCUS       DASTKS010000468         6802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_521185, whole genome shotgun sequence.
ACCESSION   DASTKS010000468 DASTKS010000000
VERSION     DASTKS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521185"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFQ35_09775"
     CDS             <1..572
                     /locus_tag="VFQ35_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930966.1"
                     /translation="GGTTAAGGGAQGGSTARGGAASGGMSNPTLVNPSFESSLTGWTI
                     DPPDAGPSGKRYVYTQAPTDSSKPPNGSQELATWHDKDSYQVTVSQTLRGLKDGIYTF
                     QGYFSSDRSGGTFIFARNCGGADREVQVPTTSWTWFTFGIKDIHVSGGSCEVGIRVIG
                     APNDYLNADMFTFEMNPEGAGGAGGAGGA"
     gene            611..2428
                     /locus_tag="VFQ35_09780"
     CDS             611..2428
                     /locus_tag="VFQ35_09780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930967.1"
                     /translation="MTQPTNAQTSSRRAGLRYERLFLLGSGGMASVHLALAVGPSGFN
                     RLVVVKSMRQELSAQSDSHQMFLAEARLSMRLNHPNVVQVSEVAEAPDGIMLVMEYLD
                     GIPLSQVIRVASDAFTLPMRLRTICEVLAGLHYAHELTEYDGSPLSIVHRDVSPQNVF
                     LTFDGRVKLLDFGIAKATTSEQTQAGVVKGRIAYMPAEQLTGDRVDRRTDIYAVGCLL
                     WELIAGSRLWANQTDREILSNVIAGHIPPLSSRVQVDPELEAIVKRATALSPGLRYRD
                     AESMRLDLEQYLSRAAAPVSTRDIGDMLSRISPAAREERQRAIAEAVAKVDVSAPASG
                     EEGLGELASFDRLKTPSTSRGSGTMLSVQLRPSRSGEHRLGHPSAAVSVREISVRDMP
                     PSSRSGPADAGHTPTKNPDTRSSLRVIAARPMPAWSIAVVALVAVVAVVAVVLFFKNR
                     GVPSGDLAAAQSPPAAVAPATRALTIDATPRDARITVDGALVPGNPAVSNVTAGSEHV
                     VRVEREGFQPSERRVQVNANLAMNVSLAAIEREPDTAPPVVSKAEPHATAGRVVRRVA
                     RPAASTPKATPSAARDASKDCDPPFYFKGGIKTYKPGCI"
     gene            2432..3343
                     /locus_tag="VFQ35_09785"
     CDS             2432..3343
                     /locus_tag="VFQ35_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930968.1"
                     /translation="MNSNRFALSGAFFFTAMVLAIAPARAESQAGSADANGSAPPTRA
                     ECLQAHQSAQELKRNEQFMEAQEQLAVCSSMSCPGAIISDCGQWISELEQRTPSMVFD
                     VQLDGKQAPNAKLFVDNQPVSEWSKAYKVNPGRHNVRAELAPFPPHEETLVLPEGQRM
                     RLVAIIFDSPKASAAPGQGDTVPPPVAVEQRRPTPGAVYPLLGVGVLGAASFTVFSLL
                     GRSEQKALERDCAPMCTSSDMSAMKRDYVIGDLSAGVGAAALIGAAIVYFTRPSETVP
                     TTGWSWNVGPAAKGDFNSFSLSAKQVF"
     gene            3415..5037
                     /locus_tag="VFQ35_09790"
     CDS             3415..5037
                     /locus_tag="VFQ35_09790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930969.1"
                     /translation="MTEDPTRQQSGHGAASALVGESGMVYRPLRELGEGGMARVYLAY
                     GQGSQGVGKLVVLKVMRESQDDFAAREMFLEEARLSARLNHPNIVQVYEVVPTGDFPY
                     MVMEYLDGKPLSKIRSGGLITQSMVLTVLSEALVGLHYAHELRDYNGTPLHIVHRDVS
                     PHNVFVTYDGAVKVLDFGIAKTDTAMARTEAGQIKGKLAYMAPEQLLGEKLDRRADVF
                     AVGAMLWEAAVGSRVWENVSQANLMHRLANGDIPKPSQRAAVDPELEAIILKAMAPDP
                     ERRYATALEMQQALESYRRRAFGSSSTRAVGAALAATFEQDREENRDLIARALHESVP
                     PPSPEISAILLSSAPPAEGASRRARLPLLIAISVAAVAGAAFLVRARSTPRPPAVAPA
                     AQPAAVQLAVRVTPPDAKIAVDGSERGVGTLVLNVQRDGRTHELSVTAPGFAPVARTV
                     RFDGDQEISIALAPVAKTDNESPRLEPSAARVESGSRPSPGGARVKARAVTPQQEQPE
                     LTKPSPATPTQANCDPPYYFKGGIKTYKPECL"
     gene            complement(5134..6678)
                     /locus_tag="VFQ35_09795"
     CDS             complement(5134..6678)
                     /locus_tag="VFQ35_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HET9930970.1"
                     /translation="MRLWVWSGLITAFVGVRTANAAPSLVNPGFENDGTGVAAPTGWT
                     SSGSVDADFTEAGGYSGSFRLSHWSAASYSVETRQKISGLSRGWYTLRARVKRSSGQN
                     DSFIALECDGRKERAYLPVAPPDTWLTIAVSVEARYGTCNVVLHTAASGGEWSNFDDI
                     ELTPGRASLSILGADVSSLAKSEDLGGYYVDASAAYQCRDSALSILARHGVNYVRLRA
                     WVNPADGYHNTRELLRMARRAKSEGLKVLVDLHYSDTWADPGQQAKPQAWAAFTTEQL
                     RQAVYDYTLDVCRRLKAQGTPPDMIQLGNELNSGMLWPDGHTWNPPNWDNLAAFLKAG
                     ASAVKASSPSTKIMLHLANGGDNGLYRWWFDNITSRGVPFDVIGASYYSYWHGSLGGL
                     QFNLNDVSARYGKDIVVVETGYPFTLADEDGWPNLIGTNDQLVSGYPATPEGQARNVR
                     DVLAIVRAVPNQRGLGAFYWEATWTAVPGNGWDPNDPTSGNAWENQALFDFNSTSLPA
                     LDVFRP"
BASE COUNT         1224 a         2213 c         2188 g         1177 t
ORIGIN      
        1 cgggtgggac gacggcagca ggaggcggcg ctcagggcgg cagcacggct cggggcggcg
       61 cagccagcgg tggcatgtcg aacccgacgc tggtcaatcc gagcttcgag tcgtcgctca
      121 ccgggtggac catcgatcca ccggacgctg gtccctctgg caagcgctac gtctacactc
      181 aggctccgac cgactcgtcc aagccgccga acggcagtca ggagctcgcg acctggcacg
      241 ataaagactc ttaccaagtc acggtttcac aaacccttcg tgggctgaaa gacggtattt
      301 ataccttcca gggttatttc agcagcgacc ggagcggcgg caccttcatc ttcgcgcgaa
      361 attgcggcgg cgcggaccgt gaagtccaag ttccgaccac gagctggacc tggtttacgt
      421 tcggcatcaa agacatccac gtttccggcg ggagctgcga agtcggcatt cgcgtcatcg
      481 gcgcgccgaa cgactacttg aacgccgaca tgttcacgtt cgagatgaac cccgaaggcg
      541 ccggcggcgc tggaggagct ggaggtgcct gagcgcaatc atggcccacc ctcaaaggac
      601 tcatagccag gtgacccagc cgaccaacgc tcagacgtcg agccgccgtg ccggcctgcg
      661 ctacgagcga ttgttcttgc tcggcagcgg aggcatggcg agcgttcact tggcattggc
      721 ggtgggcccg agcgggttca atcggctggt ggtggtgaag tcgatgcgcc aggagctttc
      781 cgcgcagagc gacagccacc agatgttcct cgccgaggcg cggctctcga tgcgcctgaa
      841 ccaccccaac gtggtgcaag tttcagaagt agcggaggcg cccgacggaa tcatgctcgt
      901 gatggagtac ctcgacggca ttccgctgtc gcaggtcatt cgcgtcgcga gcgatgcgtt
      961 cacgctgccg atgcgcctcc ggaccatttg cgaggtgctg gctgggctcc actatgcgca
     1021 cgagctcacc gagtacgacg gcagcccgct ctcgatcgtg caccgtgacg tcagcccgca
     1081 gaacgtgttt ctgactttcg acggccgggt caagctgctg gacttcggta tcgcgaaagc
     1141 gacgacgtcc gagcagacgc aggcgggcgt agtcaaaggc cgcatcgcgt acatgccggc
     1201 cgaacaattg acgggcgatc gggtcgatcg gcgtaccgac atctacgccg ttggctgcct
     1261 gctctgggag ctcatcgcgg gtagccggct ctgggccaac cagacggatc gtgaaattct
     1321 gagcaacgtg atcgcgggac acattccgcc gctcagcagc cgcgtccaag tcgaccccga
     1381 gctcgaagcg atcgtgaagc gcgcgaccgc gctcagcccg gggctgcgct accgcgacgc
     1441 ggaatccatg cggctcgacc tcgagcagta tttgagccga gcggcggcgc ccgtgagtac
     1501 gcgcgacatc ggcgacatgt tgtcccggat ttcgcctgca gcgcgtgaag agcgccagcg
     1561 cgcgatcgcc gaagccgtcg ctaaggtcga cgtcagcgcc cccgcgagtg gtgaagaagg
     1621 gctcggcgaa ctcgcttcgt tcgaccggtt gaagaccccg agcacttcgc gcggaagtgg
     1681 aacgatgctc agcgtgcagc tgcgcccgtc acgcagcggc gagcatcgct tgggacaccc
     1741 gagcgccgcg gtttcggtcc gcgagatatc ggttcgcgac atgccgcctt cgtcgcgtag
     1801 cgggcctgcc gatgcaggac atactccgac caagaacccg gatacgcgga gctcccttcg
     1861 cgtcatcgcc gcgcggccca tgcccgcctg gtcgatcgcc gtggtcgcgc tcgtcgcggt
     1921 cgtcgcggtc gtcgcggtgg tgttgttctt caagaatcgg ggggtgcctt ctggcgacct
     1981 cgctgctgct cagagtccgc ctgcagcggt cgcgccggca acccgggcgc tcacgatcga
     2041 cgcaaccccg cgcgatgcgc gcatcaccgt cgacggcgcg ctcgtccccg gtaaccccgc
     2101 cgtttcgaac gtgactgccg gctccgaaca cgtcgtgcgc gtcgagcgcg aaggctttca
     2161 gccgagcgag cgccgcgtgc aggtgaacgc gaacctggcg atgaacgtga gcctcgccgc
     2221 gatcgaacgc gagcccgata ccgctccgcc cgtcgtgagc aaagcagagc cccacgcgac
     2281 ggccggacgc gtggtacgtc gcgtggcccg tcctgccgcg agcacgccga aggcaacgcc
     2341 cagcgcggcc cgggatgcct cgaaggactg tgacccgccg ttttacttca agggcggcat
     2401 caagacctac aagccaggtt gcatttgagc catgaatagt aatcgcttcg ccctgtctgg
     2461 ggccttcttt ttcacggcga tggttcttgc gatcgcgcct gcgcgcgcag agtcgcaggc
     2521 cgggtctgcc gacgcgaacg gctcggcgcc gccgacccgc gcagagtgcc tgcaagcgca
     2581 tcaaagcgcg caggagctga agcgcaacga gcagttcatg gaagcgcaag agcagctcgc
     2641 ggtctgctcg agtatgagct gccccggcgc aatcatttcc gattgtgggc aatggatcag
     2701 cgagctcgag caacgtacgc cgtcgatggt tttcgacgtg cagctcgatg gcaaacaagc
     2761 gccgaacgcc aagctgttcg tcgacaatca gccggtctcc gagtggtcca aggcgtacaa
     2821 ggtcaatcca ggtcggcaca acgtacgcgc cgagctcgct ccgtttcccc cgcacgagga
     2881 gacgctcgtc ttgccggaag ggcagcggat gcgactcgtt gccatcatct tcgattcgcc
     2941 caaggcgtca gccgctccgg gacaaggcga cacggtgccc cctcccgtcg ccgtcgagca
     3001 gcggcgcccc acgcccggcg cggtgtatcc gctgctcggc gtcggcgtcc tcggcgctgc
     3061 gagcttcacc gtgttctcgc tgctcggtcg ctccgagcag aaggcgctcg agcgcgactg
     3121 cgccccgatg tgcacctctt ccgacatgag cgcgatgaag cgcgactacg tgatcggcga
     3181 cctctcggcg ggtgtcggtg cggcggcgct cattggcgct gcgatcgtct acttcacccg
     3241 tccgagtgaa acagtgccca ccaccgggtg gtcctggaac gtaggtcctg ccgcgaaggg
     3301 cgatttcaac tccttctctc tttccgcgaa gcaggtgttt tgacgcggcc gaaccggcat
     3361 cacggtgcgg cccggagtga cgagctgccc gaacgagacg cctcggagtt cgaggtgacg
     3421 gaggatccta ctcgccaaca atccggacat ggagccgcga gcgctttggt cggcgagtcc
     3481 ggaatggtgt accgccccct gcgagagctc ggcgaaggcg gtatggcgcg cgtgtacttg
     3541 gcctatggtc aggggagcca aggcgttggg aagctcgtcg tcctcaaggt gatgcgcgag
     3601 tcacaagacg acttcgcggc gcgcgagatg ttcctcgaag aagcccggct ctcggcgcgg
     3661 ttgaaccacc cgaacatcgt gcaagtgtac gaggtcgtgc cgacgggcga cttcccgtac
     3721 atggtgatgg agtacctcga cggcaagccg ctctcgaaga tacgtagcgg cgggctcatc
     3781 acccaatcga tggtgttgac ggtcctgagc gaggcgctcg tgggcctgca ctacgctcac
     3841 gagctcaggg attacaatgg tacgccgctt cacatcgtcc atcgggacgt gagtccgcac
     3901 aacgtcttcg tgacgtacga cggcgcggtc aaagtgctcg atttcggcat cgcaaagacc
     3961 gacacggcca tggctcgcac cgaggccggg caaatcaagg gcaagctcgc ttacatggct
     4021 ccagagcaac tcctcggaga gaagctcgac cgtcgcgcgg acgtgttcgc ggtcggcgcg
     4081 atgctgtggg aggccgccgt tggaagccga gtctgggaaa acgtcagcca agcaaacctc
     4141 atgcaccggc tcgcgaacgg cgacattccg aagccgagcc agcgcgccgc cgtcgacccg
     4201 gagctcgagg cgatcatcct gaaggcgatg gcgccagatc ccgagcgccg gtacgccacc
     4261 gctctcgaga tgcagcaggc gctcgagtcg taccgtcgtc gcgccttcgg ctcgtcctcg
     4321 actcgcgcag tgggagcggc gctcgccgcg accttcgagc aagaccgcga ggagaaccgc
     4381 gacctgatag cgcgagcgtt gcacgagtcg gtgcctccgc cgtcccccga aattagcgct
     4441 attttgctgt cgagcgctcc gcccgcggaa ggtgcgagca ggcgagcgcg tctgcctttg
     4501 ctgatcgcga tcagcgtcgc ggccgttgcg ggcgccgcgt ttttggtgcg tgcgcgctct
     4561 actccgcgcc ctccagcggt tgccccggcg gcgcaaccag cggctgtgca gctcgccgtc
     4621 cgcgtgacgc ccccggacgc caagatagcg gtggacggca gcgaacgcgg cgtgggcacc
     4681 ctcgtgctca acgttcagcg cgatgggcgc actcatgagc tcagcgtgac cgcgccgggg
     4741 ttcgcgcccg tggcgcgcac tgttcgcttc gacggcgacc aagagatcag cattgctctc
     4801 gcaccggtcg caaagacaga caacgagtcg cctcggctcg agccgagcgc cgcccgcgtc
     4861 gaaagcggaa gcaggccatc gccaggtggg gccagagtga aggcccgcgc ggttacgccc
     4921 caacaagagc aaccagagct gaccaagcca tcacctgcga ctccgactca ggcgaactgc
     4981 gacccgccct actacttcaa aggcggcatc aagacctaca agcccgagtg cctgtagagt
     5041 agttcgcgcc gcgcgatttg ctcccggatg agcgcgagcg aaaccgccga cttcgaatgg
     5101 tcggcggttt cgtcacggat caggcgagtg tgcttacggc cgaaacacgt cgagcgcggg
     5161 gagagaggtc gagttgaagt cgaacagggc ctggttctcc caagcgttgc cgctcgttgg
     5221 gtcgttcggg tcccagccgt ttcctggcac tgcggtccac gtcgcttccc agtagaaagc
     5281 cccgaggccg cgctgattcg gcaccgcgcg cacgatcgcg agcacgtccc gaacgttgcg
     5341 agcttggccc tccggtgtcg cgggataccc cgaaacgagc tgatcgttgg tgccaatcaa
     5401 gttcggccag ccatcttcgt ccgccagcgt gaatggataa ccggtctcga cgacgacgat
     5461 atccttcccg tagcgcgccg aaacgtcgtt gagattgaat tgcaggcctc cgagtgaccc
     5521 atgccaatac gagtagtacg acgcgccgat gacatcgaag ggcacgccgc gcgacgtgat
     5581 gttgtcgaac caccagcggt agaggccgtt gtccccgccg ttcgcgagat gcagcatgat
     5641 tttggtcgac ggagagctcg ccttcacggc gctcgcacct gctttcagaa aagcggcgag
     5701 gttgtcccaa ttcgggggat tccaggtgtg accgtcgggc cacagcatgc cggagttcag
     5761 ctcgttgcct agctgaatca tgtccggcgg cgtgccctgc gctttcagtc gccggcaaac
     5821 atcgagagtg tagtcgtaca cggcttgccg aagctgctcg gtggtgaacg cggcccaagc
     5881 ctgtggcttt gcctgctggc ccggatccgc ccacgtatcc gaatagtgca aatcgaccaa
     5941 gactttgagg ccctcgctct tggcccggcg cgccatcctc aagagttctc tcgtgttgtg
     6001 atagccgtcg gcagggttca cccaggcgcg cagccgaacg tagttcactc catgccgagc
     6061 gagaatggag agggcagagt ctcgacattg gtacgcggcc gaagcgtcta cgtagtagcc
     6121 gccgaggtcc tcggactttg ccaggctcga gacgtcagcg ccgagtatgg agagcgacgc
     6181 gcgcccaggc gtgagctcga tgtcatcgaa gttcgaccac tcgcctcccg aagcggcggt
     6241 atgcagcacg acattgcacg tcccgtagcg cgcctcgact gataccgcga tggtgagcca
     6301 cgtatctggc ggagccaccg gcaagtaggc gcgctccttg cgcccatcgc actcgagagc
     6361 gatgaagctg tcgttttgcc ccgagctacg tttcacgcga gcccgaagtg tgtaccagcc
     6421 gcgactcaac cccgagatct tctgccgagt ttcaacgctg tagctggcgg cactccaatg
     6481 actcaggcga aagctaccgc tatatcctcc cgcctcggta aaatccgcat cgacggagcc
     6541 gctgctcgtc catcccgtgg gcgcggctac gccggtccca tcgttctcga agccggggtt
     6601 gacgagcgat ggagccgcgt tggcggtgcg aacgcccacg aacgcggtga ttagaccgga
     6661 ccaaacccaa agtcgcatcg gttccctcct cagttgtcag aacattcata tcacgcatcc
     6721 accctggagg gtagctttgc gtttcgttta tctcgctcac gtcgtgcgtc gtcattgagg
     6781 tgcaaaccat gtgggtgagt cc
//
LOCUS       DASTKS010000469         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_743100, whole genome shotgun sequence.
ACCESSION   DASTKS010000469 DASTKS010000000
VERSION     DASTKS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743100"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VFQ35_09800"
     CDS             complement(<1..806)
                     /locus_tag="VFQ35_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930971.1"
                     /translation="MARRILSSSSAIALVVCSACGLFDFDRPRLREGAGGAAGQPSFG
                     GMLNAQGGSGEAGSGGEAATMGGANGGVSGSASSGGGEPGGDAGSERGGAASSSGGAG
                     TNGFSSGGLIGASGATANSGGLGAALGGTNSGGRPFTATGGSASGGVSGALGGTANFG
                     GVGGSAGEGCASWSTFPAPESIVRDDFAGTSLDPRWKNLNASSSQKYSVSDGQLSSSA
                     GIDDAMFWSEKQGANQEAYARILSFDPLLYGTVLLLKANRQDVQRFAISI"
     gene            complement(788..1795)
                     /locus_tag="VFQ35_09805"
     CDS             complement(788..1795)
                     /locus_tag="VFQ35_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930972.1"
                     /translation="MALASPDLARAQSAAQIAAEARFTEGVRLMKADRCEAALTEFRA
                     SQALDPASGTLLDIGYCEVRLGHVASAWFAYRQALSLAQASRKEHHERIARRELDALE
                     PQLAYWSLRVPAGSADVSVEVDGSRVEREVWSAGTPADPGAHQIRVFERGAPKWERSF
                     SLVAGQRLVLDVPGEAFAARAPEVLAAPRVSETAVEVPMSPAGVGAREPSQVASAARE
                     VQRHDARRILALSSAGAGAACLLGAGILFAKARHDYDAVDEDCPSNVCRSKSSYEARN
                     GAIDLFHWSLGVGGVGVALLGVGSALLLSSRASADVSVARSISFQLDARKAELTWRGA
                     F"
     gene            complement(1819..>3077)
                     /locus_tag="VFQ35_09810"
     CDS             complement(1819..>3077)
                     /locus_tag="VFQ35_09810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930973.1"
                     /translation="HLELDQLVAIKMLLPATSQDRAAAVRLLREARAAARIKSDHVVR
                     VFDVTATGEFSPYIVMEYLEGESVGRRVQRAGALSVAETADIVIQAAEAIAEAHALGI
                     VHRDLKPENLFITSKPGHPRFVKVLDFGISKSQAGLGEQLTQSHALLASPAYAAPEQL
                     RSSRTAGPAADIWSLGVILYECATGTRPFLGDTLAEICTQVLYEPLVSPRVVRAELPP
                     EFDTVIMRCLEKEPGRRFASIEELVVALAPFAPESAKAFVAHTAPARAEGTHGPVGAG
                     GGKGNSTVTAIETRLGDVPRARSLRQAGLAAAGLVFGAAVLTVWVRRSNAPQVASAAA
                     PLVASRAPALPSTLPVASSATPSELHDAATSPSAGATASSSAAPNAQRVPNPHSPSVA
                     PKPPKRKNNQPAIKTGSTNESWVETR"
BASE COUNT          512 a         1094 c          967 g          504 t
ORIGIN      
        1 tagatcgaaa tcgcgaatcg ctggacgtcc tggcgattgg ccttcagcag cagaaccgtt
       61 ccatagagca acggatcgaa cgagagaatc cgcgcatagg cttcttggtt cgcaccctgc
      121 ttttcgctcc agaacatcgc gtcgtcgatg ccggccgaag acgagagctg cccgtcggac
      181 accgagtatt tttgggagga gctcgcgttc aggttcttcc agcgcgggtc gagcgatgtg
      241 cccgcgaagt catcccgaac gatgctctcg ggagccggga acgtggacca actcgcacaa
      301 ccctcgccag cacttccgcc gaccccgccg aagttcgccg ttcccccgag cgcgccgctg
      361 acgccaccgc tcgcggaacc acccgtggcc gtgaaaggtc gcccgccgga gttggtccct
      421 cccaacgcgg cgccgagccc gcccgagttt gcggtcgctc ccgaggctcc gatgagccct
      481 ccactcgaaa acccgttcgt gccggctccc ccactcgacg acgcggcgcc tccgcgctcc
      541 gaacccgcat ctcctcctgg ctcacctccc ccggacgacg cgcttccact aacgccaccg
      601 ttggcgcctc ccatcgtcgc agcttcgccg ccgctgcctg cttcaccgct tccgccctgc
      661 gcgttcagca taccgccgaa cgacggctga ccagccgcgc cgcccgcgcc ttctctcagc
      721 cgaggtcggt cgaagtcgaa tagcccgcac gcggagcaaa ccaccagcgc gatcgccgat
      781 gacgaactca gaatgcgccg cgccacgtaa gctccgcctt tcgcgcgtcg agttgaaacg
      841 agatgcttcg cgccacgctg acgtcggccg acgctcgcga gctcagcagc agcgccgatc
      901 cgacacccag gagcgcaacg cccacacctc cgacgccgag cgaccaatgg aacaagtcga
      961 tcgcgccgtt ccgcgcctcg tagctcgact tcgagcgaca aacgttcgac gggcagtcct
     1021 cgtcgacggc gtcgtagtcg tggcgcgcct tcgcgaacag aattcccgcg ccgagcaaac
     1081 aggccgcgcc agcaccggcg gacgacagag cgaggatgcg acgcgcgtcg tggcgttgga
     1141 cttctcgagc cgctgaagcg acctgactcg gctctcgcgc tccaaccccc gcgggcgaca
     1201 tcggtacctc gaccgcggtt tcgctcactc gcggtgcggc cagaacttcg ggcgctcgtg
     1261 cggcgaacgc ttcgcctggc acgtcgagaa ccaagcgctg ccccgcgacg agagaaaagc
     1321 tcctttccca ttttggcgcc ccgcgttcaa agactctgat ctggtgtgct cctggatctg
     1381 ccggagttcc tgccgaccac acctcccgct cgacgcgcga gccatccacc tcgaccgaca
     1441 cgtccgcgct gcccgcgggg actcgtagcg accagtacgc tagctggggt tcgagcgcgt
     1501 cgagttcgcg ccgcgcgatg cgctcgtggt gctctttgcg gctcgcctgc gcgagcgaca
     1561 gggcttgccg gtacgcaaac caggcgctcg cgacgtgacc gagccgcacc tcgcagtatc
     1621 cgatgtcgag caaggtgcca ctggcggggt cgagcgcttg actcgcgcga aactcggtga
     1681 gtgcggcttc gcagcgatct gccttcatca agcggacgcc ttcggtgaac ctggcctcgg
     1741 ccgcgatctg cgccgcgctc tgggcccgcg cgaggtcggg cgaagcgagc gccaccgaac
     1801 ctatggcgaa aaaaagtgtc agcgcgtttc tacccatgat tcgttcgtcg atccggtttt
     1861 gattgcgggc tgattgtttt ttcgcttcgg cggcttgggc gcgaccgacg gcgaatgcgg
     1921 attcggcacc cgctgcgcat tgggcgccgc ggagctcgag gcggtggctc cggcgctcgg
     1981 cgaagtcgca gcgtcgtgca gctccgaagg cgtggccgaa ctcgcgaccg ggagagtact
     2041 cgggagcgcg ggcgcgcgtg aagcaacgag cggggccgcg gccgatgcca cctgcggagc
     2101 attgctccgc cgcacccaaa cggtgagcac cgccgcgccg aatacgaggc ccgctgcagc
     2161 taacccggct tggcgcaacg acctggcccg cggaacgtcg ccgagccgcg tctcgatcgc
     2221 ggtgacggtc gagttaccct ttccaccccc cgctcccacc ggtccatgcg tcccttcggc
     2281 gcgcgctggc gcggtgtgcg caacgaaggc cttggccgac tcgggcgcga acggcgcgag
     2341 cgccaccacg agctcctcga tactcgcgaa gcgccggccc ggctctttct ccaggcatcg
     2401 catgatcacg gtgtcgaact ctgggggtag ctccgcccgc acgacgcgcg gcgataccag
     2461 gggctcgtac agaacttggg tgcaaatctc cgccaacgtg tcgccaagga acggacgcgt
     2521 gcccgtagcg cattcgtaga ggatcacgcc gagagaccag atgtccgcag cgggaccggc
     2581 agtccgcgag gagcgaagct gttccggcgc cgcataagcc ggagacgcga gcaatgcatg
     2641 actttgcgtg agctgctcgc ccaaccccgc ttgcgacttg gaaatgccga aatccagcac
     2701 cttgacgaag cgcgggtgtc caggcttgct cgtgatgaac aggttttccg gcttcaggtc
     2761 gcggtgcacg ataccgagcg cgtgagcttc ggcgatggcc tccgcggcct ggatgacgat
     2821 gtccgcggtt tcggcgacgc tgagcgctcc ggcgcgttgc acgcgtcgac caacgctctc
     2881 gccctcgagg tactccatga cgatgtacgg cgagaactct ccggttgccg ttacgtcgaa
     2941 gacccgaacg acgtggtcgc tcttgattcg agccgctgct cgcgcttctc ggagtagcct
     3001 caccgcggcc gcgcgatcct gcgaagtcgc cggcagcagc atcttgatcg cgacgagctg
     3061 gtcgagctcg aggtgac
//
LOCUS       DASTKS010000470         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1414293, whole genome shotgun sequence.
ACCESSION   DASTKS010000470 DASTKS010000000
VERSION     DASTKS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414293"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2524)
                     /locus_tag="VFQ35_09815"
     CDS             complement(<1..2524)
                     /locus_tag="VFQ35_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9930974.1"
                     /translation="MISRIFIDRPIFAWVIAIAIMLAGIGSIFLLPMEQYPDVAPPSV
                     NIRATYPGASADTLETSVTQVLEQQLTGLSGLMYFSSSSSSQGSASITATFVKGTDPD
                     IAQVQVQNKIQQAISRLPQIVQQLGVIVTKSNPDFLLIIAVYDVTDRATSSDVSDFLV
                     STMQEPIGRVSGVGDLRVFGSQYAMRIWLDPLRLATAELMPSDVRAAILAQNTQVAAG
                     QIGAQPAPPEQMLNATVLAKARLQTPEQFRKIILKTRPSGSQVLLGDVARVELGSEDY
                     STVTRVNGHPGSGMAIMLAPGADALQTAEAVKAEVARRAPGFPPGYEYAFPRDSTAFI
                     RLSIEEVVKTLFEAIVLVVIVMFIFLQSWRATLIPAIAVPVVLLGTFGALQAFGFSIN
                     TLTLFGLVLAIGLLVDDAIVVVENVERVMAERPGISAREATIASMEEIQTALIAIALV
                     LSAVFLPMAFFGGSTGVIYRQFSITVVSAMVLSVIVALILTPALTAQLLRPRAHEQRD
                     AVEPPDAREQRRELPAERRARRIGSRLRNGFNRAFERTTNVYVRGVRGVLGRKGAFLL
                     GYAVLAGALVFTFLRIPTGFLPSEDDGTAMVQFTLPAGATQSRTSKVAGSVEAYFLGP
                     EKKNLAALFTVLGFGFNGQGQNNGMGFLSLAPWEERPGPENSSPAITRRATAGLSGVR
                     DAQIVALTPPPVRGLGQSSGFTMELLNSSGMPRDEFIALRNQLLGRAMSDPRLAAVRP
                     SELPDTPSLHVTMDEAKAGALGISPDDAFTTLSFAWGGTYVNDFVDRGRVKRVYLQGD
                     APYRSEPADIGKWYVRSKSGTMAPLSAFAQSDWENSPVVRSRF"
     gene            complement(2521..>3763)
                     /locus_tag="VFQ35_09820"
     CDS             complement(2521..>3763)
                     /locus_tag="VFQ35_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9930975.1"
                     /translation="VRHSPSMPVRDGCRASPSSQPVSVLLADVVRLLRSAALIALTCS
                     CHPKQKGPEPAPRPVEVGVVVLRAESVLLTTELPGRTSAYETSEVRPQVSGIVRARTF
                     TEGQVVKQGQTLYQIDARPYRAAEAQARANLVSAQAATGAARARAERYESLVQIQAVS
                     EQDYADAKASAEQAVAAVEQARAALQTASINLQYTTVAAPIAGRIGRSLVTTGALVTA
                     NQAQPLATIQRLDPIFVDLQQSTADLLALRRSLSEGGALAASADVTLVLEDGSNYSRN
                     GKVQFAEATVDPSTSAVTLRARFDNPDGVLLPGMYVRALVTQAQRPSAILAPQPGVTR
                     DPKGNATALVVGPDDKVVLRQIRTSRVVRDMWLVDEGLAAGDRLIVEGTVKVKPGQRV
                     KPVPAGERAASSAAPQPSARP"
BASE COUNT          657 a         1190 c         1240 g          676 t
ORIGIN      
        1 tgaaccgcga gcgcacgacg ggcgagttct cccaatcgct ttgggcgaaa gcagagagcg
       61 gcgccatcgt cccgcttttg gagcgcacgt accattttcc gatgtccgct ggttcggagc
      121 gatagggcgc gtcgccctgc aggtacactc gtttgacccg gccccgatcg acgaagtcgt
      181 tgacgtaggt gccgccccac gcgaacgaaa gggtggtgaa agcatcgtcc ggagagatcc
      241 ctagggctcc cgctttcgct tcgtccatcg tcacgtgcaa cgacggcgtg tcggggagct
      301 cgctggggcg cacggccgct agccgtgggt ccgacatcgc tcgcccgagg agttggttcc
      361 gaagcgcgat gaactcgtca cgaggcatgc ccgacgagtt caagagctcc atcgtaaagc
      421 cgctcgactg gccgaggccg cgtaccggtg gcggagtgag cgcgactatt tgagcgtcgc
      481 gcacgccgct gaggccggcc gtggctcggc gcgtgatggc aggcgacgag ttctctggcc
      541 caggccgctc ttcccatggc gcgagcgaca gaaagcccat tccattgttt tgcccctgac
      601 cgttgaaccc gaacccgagc accgtgaaca gcgcggcgag gttctttttt tcgggtccga
      661 ggaagtacgc ttcgaccgat cccgccacct tcgacgtgcg gctctgggtc gcgccagcgg
      721 gcaacgtgaa ttgcaccatc gcggtgccgt cgtcttcgct cggcagaaat ccggtgggga
      781 ttcgcaagaa cgtaaacacc agcgcgccgg cgagcaccgc gtaccccaag aggaacgcac
      841 ccttcctccc gagaacgccc cggacgccgc gcacgtagac gttcgtcgtg cgctcgaaag
      901 cgcggttgaa accgtttcgc agacgagaac caatgcggcg ggctcgccgc tcggccggga
      961 gctctctccg ctgctcgcgg gcgtcgggcg gctcgaccgc atcgcgctgt tcgtgcgcgc
     1021 ggggcctcaa caattgtgcg gtgagcgccg gggtgaggat gagcgccacg atcaccgaca
     1081 agaccatggc cgagacgacg gtgatcgaaa actggcgata gatgacgccg gtcgagccgc
     1141 cgaagaatgc catcggcaaa aagaccgccg acaataccag tgcgatggcg atgagggcgg
     1201 tctggatctc ttccatggaa gcgatcgtag cttcgcgcgc gctgatgccg ggtcgctccg
     1261 ccatcactcg ctcgacgttc tctacgacga cgatggcgtc atccaccaga agcccgatcg
     1321 cgagcacgag gccgaacagc gtgagcgtgt tgatcgagaa gccgaatgcc tgcaacgcac
     1381 cgaaagtgcc caggagcacg actggcaccg cgatggcggg gatgagcgtc gcgcgccagc
     1441 tctgcaagaa gatgaacatc acgatcacga ccagcacgat ggcctcgaat agggtcttca
     1501 cgacctcctc gatggacagc cgaatgaagg cggtgctgtc gcgcggaaac gcgtactcgt
     1561 agccaggggg gaagccgggc gcgcgacgag ctacttcggc cttcactgct tcggcggttt
     1621 gaagcgcgtc cgctccaggg gcgagcatga tcgccatgcc ggagccggga tgcccattca
     1681 cgcgagtcac ggtgctgtag tcctcgctcc cgagctcgac gcgcgcgacg tcgccgagca
     1741 gaacttgcga gccgctcggc cgcgtcttga ggatgatttt gcgaaactgc tcgggcgttt
     1801 gtagccgcgc cttggcgagg acggtcgcgt tcagcatctg ctctggcggc gcgggctgtg
     1861 cccctatttg ccctgccgcg acttgcgtgt tctgggccag aatggcggcg cggacgtcgg
     1921 atggcatgag ctcggccgtc gccagccgca gcggatcgag ccagatgcgc atcgcgtact
     1981 gagagccgaa gacccgcaga tctccgaccc ccgatactcg tcctatcggc tcctgcatgg
     2041 tcgaaacgag gaagtccgag acatcgctcg acgtcgcgcg atcagtcacg tcgtacaccg
     2101 cgatgatgag taagaagtcg gggttcgact tggtcacgat gacgcctagc tgctgaacga
     2161 tttgtggcag gcgcgatatt gcttgctgga tcttgttctg cacctgcacc tgggcgatat
     2221 ccggatccgt gcctttgacg aaggtcgcgg tgatcgaggc ggaaccctga gagctcgaac
     2281 tcgagctgaa gtacatgagc ccgctgagtc ccgtcagctg ctgctcgagc acttgcgtga
     2341 cgctggtctc tagagtgtcg gcggaagctc ccgggtaggt cgcgcggatg ttgacgctcg
     2401 gaggcgcgac gtccgggtac tgctccattg gcagaaggaa gatcgagccg atgccggcga
     2461 gcatgatggc gatggcgatc acccacgcga agatcgggcg atcgatgaag atccgcgaaa
     2521 tcatggcctt gcgctcggtt gcggcgccgc cgaggaagcg gctcgttcac cggctggtac
     2581 tggcttcaca cgttgccccg gcttcacctt gacggtgcct tcgacgatca atcgatcccc
     2641 cgcggcgagt ccctcgtcga cgagccacat atcgcgcacg acacgcgacg tccggatttg
     2701 tcgaagcacg accttgtcgt cggggccgac caccaaagcg gttgcattgc ccttggggtc
     2761 gcgggtcact ccgggttgcg gtgccaagat ggcggagggt cgttgggcct gggttaccag
     2821 cgctcgcacg tacatccctg ggagcagcac gccatcgggg ttgtcgaaac gcgcccgcag
     2881 cgtcaccgcg ctcgtgctcg gatcgacggt cgcttcggcg aattggactt tcccatttct
     2941 cgagtaattg ctgccgtcct cgagcaccag cgttacgtcg gccgacgctg cgagcgcgcc
     3001 accttccgaa agcgaacgcc gaagcgccag tagatcggcc gtggactgct gcaggtcgac
     3061 gaatatcggg tcgagccgct gaatcgtcgc caacggctgc gcttggttgg cggtgacgag
     3121 cgcacccgtc gtcacgagcg agcggccgat gcgtcccgcg atcggcgccg ccacggtcgt
     3181 gtattgtaag ttgatgctgg cggtctgaag ggccgcacgt gcttgctcta cggccgctac
     3241 ggcttgttct gcgctcgcct tcgcatctgc gtaatcttgc tcactgacgg cctggatctg
     3301 cacgagcgac tcgtaacgtt cggcccgcgc gcgcgcggct ccggtggcgg cttgggcgct
     3361 cacgaggttc gctcgcgctt gcgcctcggc ggctcggtac gggcgggcat cgatctggta
     3421 gagcgtctgc ccttgcttta cgacttgtcc ttcggtgaag gtccgagcac ggacgatgcc
     3481 tgatacttgc ggccgcactt cggaggtctc atacgcggaa gtcctccccg ggagctccgt
     3541 cgtgagcaac accgactctg cccggaggac gaccaccccg acctcgaccg gtctcggcgc
     3601 aggttctggg cccttctgct tcgggtgaca gctacacgtg agcgcaatca gcgccgcgct
     3661 ccgcaatagc ctcacgacgt ctgcaaggag cacgctcacg ggctgtgagg aaggtgaagc
     3721 gcggcagcca tcccttaccg gcatcgacgg agaatggcga acg
//
LOCUS       DASTKS010000471         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1860826, whole genome shotgun sequence.
ACCESSION   DASTKS010000471 DASTKS010000000
VERSION     DASTKS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860826"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..>2430
                     /locus_tag="VFQ35_09825"
     CDS             209..>2430
                     /locus_tag="VFQ35_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HET9930976.1"
                     /translation="MKNKMPTREEIVRRLQEAGRPLTARELKAAFSLLPEQDLGGVLE
                     AMVTRGLLKRLGGHRYAPAAIGRSVDSTIGKLSVNPRGFGFVTAPGAEDVYIAPESVG
                     AALHGDRVEYEIVGRSARGAEGRIVRIVERRNSRIAGLLRRRGKSVWLEPDDSRIRGP
                     IVVSAGQKIGRDGEAAVVSITRFPEFPDENAEGELVSVLGTPGEPNVEVAKILVREGV
                     EEQHSDGAVREAESLAARRLREVPEWRRDLRNVPLPTIDPEDARDHDDAIWVERNGEG
                     YRAYVAIADVSESVEEGSALDKEARARGCTIYLPDRAIPMLPSALAADLCSLLPERDR
                     LCLCVIVELDANGNVKSFEIVEGVMRSAAMLTYGGVARALGFTDEPPRSAQAEAMKKD
                     LKVLDELARKLRRTRMARGALDLDLPEPKLVLDPKTGAPLDVQRRTQDPGIKRAYSMV
                     EELMLLANEVVAQWLGERRSAAVYRVHGKPDEAKLERLSGIAEKLGVELDLEALQTAS
                     GVSRWLSKIQKHPRRQVLEMLLLRSLKQATYDTKNMGHFGLASEAYVHFTSPIRRYPD
                     ILVHRAVKHLLRGNKPKNAPDALESLAEAAQLSSERERAAMEIEREVVDLYRTLLMQQ
                     HVGDVLEGTVTALVGSGCFVALDQPFVEVLVRFEAMGPDRYELSDDELSVVGARSGDS
                     IALGDRMVVVIEDVAVLWRTVYARRVVPEAVLEKVSEAEPPTRGRKKVVEAPPRRGFV
                     "
BASE COUNT          421 a          789 c          854 g          366 t
ORIGIN      
        1 cgcagaatgc cgcgtccgcg ctgcgatatt cgacgctgag cgctgcggat gccgctagtg
       61 ccagcagggt cgtaatgcga aggaccagca gggccggaat acgcgccacg ggtcccagtc
      121 gtatacgtcg cgcgcgcggg gccgaccagg gtccatttca agaaagcgcg ggccccacga
      181 gcgccggcgg gctatagcgg gcaccgacat gaaaaataag atgccgacgc gcgaagagat
      241 cgtgcgtcgt ctacaagagg cgggaaggcc tctcacggcg cgcgagctaa aggccgcttt
      301 ctcgctgcta ccggaacagg atttgggggg cgtgctcgaa gcgatggtca cgcgagggct
      361 cttgaagcgc ctcggcgggc atcgctatgc cccggcggcc atcggacgct cagtcgactc
      421 gaccatcggc aagctcagcg tgaacccgcg cggtttcggt ttcgtcaccg cgcccggcgc
      481 ggaagacgtc tacatcgctc ccgagtcggt gggcgcagcg ctgcacggcg atcgcgtcga
      541 gtacgagatc gtcggtcgtt cggcgcgcgg tgccgagggc cgaatcgtcc gtatcgtcga
      601 gcgccgcaac tcgcgcatcg cggggcttct gcgacgccgc ggcaagagcg tttggctcga
      661 gccagacgac tcccgcattc gcggccccat cgtcgtcagc gccggccaga aaatcggccg
      721 agacggcgag gcggcggtgg tcagcatcac gcgctttccg gagttccccg acgaaaacgc
      781 ggagggtgag ctcgtctccg ttctcggcac gccaggcgaa cccaacgtcg aggtcgcgaa
      841 gatcctcgtg cgcgaaggcg tcgaggagca acactcggac ggcgccgtgc gcgaagccga
      901 atcgctcgcg gcgcggcgcc tgcgcgaggt gcccgagtgg cgacgcgatc tccgcaacgt
      961 gccgctgccg accatcgatc cggaagacgc gcgcgaccat gacgacgcga tttgggtcga
     1021 gcgcaacggc gagggctatc gcgcgtacgt cgccatcgcc gacgtgagcg aatcggtcga
     1081 agagggctcc gcgctcgaca aggaggcccg cgcccgtggc tgcacgattt acctgccgga
     1141 tagggccatt ccgatgctgc cgagcgcgct cgcggcggac ctgtgttcgc tcttgccgga
     1201 gcgggaccgg ctctgcctgt gcgtgatcgt ggagctcgac gcgaacggta acgtgaagtc
     1261 gttcgagatc gtcgagggcg tgatgcgctc ggcggcgatg ctgacgtacg gcggcgtcgc
     1321 gcgcgcgctg ggcttcaccg acgagccgcc gcgcagcgct caagccgaag cgatgaagaa
     1381 ggacctgaag gtcctcgacg agctcgcgcg aaagctccgc cgcacccgca tggcgcgcgg
     1441 cgcgctcgat ctggacctac cggagcccaa gctggtgctc gatcccaaga ccggcgcgcc
     1501 gctcgacgtg cagcgccgga cgcaagatcc cggcatcaaa cgcgcctatt cgatggtcga
     1561 ggagctgatg ctgctcgcga acgaagtcgt ggcgcagtgg ttgggggagc ggcgcagcgc
     1621 ggccgtgtac cgcgtccacg gcaagcccga cgaagcgaag ctcgagcgcc tttcgggcat
     1681 cgccgagaag ctcggggtgg agctcgatct cgaagcactt cagacggctt cgggcgtttc
     1741 gcgctggctg tccaagatcc aaaagcaccc gcgccggcag gtgctcgaaa tgctgctcct
     1801 gcgttcactg aagcaggcga cgtacgacac caagaacatg gggcacttcg gccttgcgtc
     1861 ggaggcgtac gtgcacttca cgtcgccaat ccggcgctac cccgacatcc tcgtgcatcg
     1921 cgcggtgaag catttgctcc gcggaaacaa gccgaaaaac gcgcccgacg cgctcgagtc
     1981 gctcgcggag gcggctcagc tctcgagcga gcgcgaacgc gccgcgatgg agatcgagcg
     2041 ggaggtcgtc gacctctacc gcacgctgct catgcagcag cacgtgggcg acgtgctcga
     2101 gggtaccgtc acggcgctgg tcggcagcgg gtgtttcgtc gcgctcgacc aacccttcgt
     2161 cgaagtgctg gtgcgcttcg aagcgatggg accggatcgc tacgagctct ccgacgacga
     2221 gctcagcgtc gtcggtgctc gctccggcga ctcgatcgca ctcggcgatc gcatggtggt
     2281 cgtcatcgaa gacgttgcgg tcctgtggcg caccgtgtac gcgcgccgcg tggttccgga
     2341 ggcggtgctc gaaaaagtca gcgaagccga gccgcccacg cgcgggcgaa agaaggtggt
     2401 ggaggccccg ccgcggcgcg gtttcgtcaa
//
LOCUS       DASTKS010000472         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_297549, whole genome shotgun sequence.
ACCESSION   DASTKS010000472 DASTKS010000000
VERSION     DASTKS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297549"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VFQ35_09830"
     CDS             complement(<1..1959)
                     /locus_tag="VFQ35_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET9930977.1"
                     /translation="MSKNRYLKSATAARVALAVLLLGVALSAALAKHQAEQNARALDE
                     RFRTITDEMANRLLQRLHTYEYGLRGAAGTVLVAGHPQVGRERYNAYAATRDISREFP
                     GARGMGIIRRIRPEDEQQFLAAARADGWPDFHIRELGPNPGERFVIQYIEPVAPNRAA
                     VGLDIASEPRRREAAVAAKDAGKATLTAPITLVQAAGKKQRSFLLLLPIYVANRLLDT
                     PEQRDAASVGWAYAPLVMDEVLADFKIGGAYSLELRDTQSRGDGAFFTSYAKEAPPAT
                     ELERRIPIAIYGRQWEARVRATPAFAHSLPLPKPTTVFGFGLGSSILLGFLSFLFVHG
                     RRRAAQLRAEQARRAAIVDSSQDAVIGETLDGIVFEWNRGAEKLFGYSAEQALGKASA
                     ALILPPDRVEEDAALRASVARGEQLPPFDTTRLTADGRLLDVSITVSSIGGDGGHMGF
                     SKTIRDISDAKRAEQAVRSLNQSLEQQVADRTLLLESARRDLQNILDAIPSLVSYWDR
                     DLRNRFANQAYRSWFGWDPESMPGRAALELLDGRHVGGEERVRAALRGEPQIFELCFE
                     SEAGSLRHAVAHLLPDTVNGEVRGFYAIVFDVTQQKEAQARLAGALRENEALLRTLHE
                     YAIVSVTDRAGRIIEANEHFIRISGYEPS"
     gene            complement(1938..2957)
                     /locus_tag="VFQ35_09835"
     CDS             complement(1938..2957)
                     /locus_tag="VFQ35_09835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9930978.1"
                     /translation="MSADILLVDDDPSAIQLLGRILAGEGTVRFATKGADAVRLASTK
                     APDLVLLDAELPDMNGFQVCTELKRTPGLEEVPVIFVTSHRDESFEVKGFDVGAVDFI
                     AKPVNPKLVLARVRAQLRNKRMADALRLSATTDALTGLANRRRFEETLEIEWRRSRRS
                     KLPLALLLVDVDHFKLFNDTYGHPEGDTCLHAVATALKSCCLRPADTVARYGGEEFAL
                     LLPETPNVGALHVAERVLSAMAELAVPHGASPTARVVTVSVGVATNENLGEPQQGSEL
                     RNAKIAQYSSRLLLQAADRALYQAKASGRARACSIDIPRTSSSALHGVEVREGEGGPC
                     PRTDT"
BASE COUNT          564 a         1072 c         1065 g          573 t
ORIGIN      
        1 gctcggctcg taaccgctga tgcgaatgaa gtgctcgttg gcctcgatga tgcgcccagc
       61 gcggtcggta accgagacga tggcgtattc gtgcagggtg cgcagcaacg cttcgttttc
      121 cctcaacgcg cccgcgagcc gcgcctgcgc ctctttctgc tgcgtgacat cgaacacgat
      181 ggcgtaaaag ccgcgcacct caccattcac ggtgtcgggc aagagatgcg cgacggcatg
      241 gcgaaggctc ccggcttcgc tttcgaaaca aagctcgaaa atctgcggtt cgccgcgcaa
      301 agccgcgcgg acccgctcct ctccacccac gtgtcggccg tcgaggagct cgagcgcggc
      361 gcgtcccggc atgctctccg ggtcccaacc gaaccagctg cgataggcct gattcgcgaa
      421 acggttgcgc aggtcgcgat cccagtagct gaccagtgaa gggatggcgt cgagaatatt
      481 ttgcaggtcg cgacgagcac tctcgagcag cagcgttcga tccgccactt gctgctccag
      541 gctttggttg agagaacgca cggcttgctc ggcgcgcttc gcgtcgctga tatcgcgaat
      601 ggttttcgag aagcccatgt gcccaccgtc tccgccgatg gacgaaacgg tgatggatac
      661 gtcgagcagc cgtccgtcgg cggtgaggcg cgtggtgtcg aagggcggaa gctgttcgcc
      721 gcgcgcgacc gaagcgcgca gggctgcgtc ttcctcgaca cggtccgggg gcaggatgag
      781 cgcggcggaa gccttgccga gcgcttgctc ggcggagtaa ccgaagagct tctccgcccc
      841 tcggttccat tcgaacacga tgccgtccaa tgtctcgccg atgacggcgt cctgcgagct
      901 atccacgatg gcggctcggc gcgcctgctc ggcgcggagc tgcgcggcgc gccggcgccc
      961 gtgcacgaag agaaagctca ggaatccgag caatatggag cttccgaggc caaagccaaa
     1021 caccgtagtg ggcttaggga gcggcaagct gtgcgcgaag gccggcgtcg ctcgcaccct
     1081 cgcctcccac tggcgaccgt aaatcgcgat cggaatcctt cgttcgagct cggtcgcggg
     1141 aggcgcctcc ttcgcgtagc tcgtgaagaa cgcgccgtct ccacgagact gcgtgtcgcg
     1201 cagctcgagt gaataggcac cgcctatctt gaagtccgcg agcacctcgt ccatcacgag
     1261 cggtgcgtaa gcccaaccaa ctgacgcagc gtcgcgctgc tcgggggtgt cgagcagccg
     1321 attggcgacg tagatcggga gcagcagcaa gaaagagcgc tgcttcttac ctgccgcctg
     1381 aactagcgtg atgggcgcgg tgagcgtggc tttgccagcg tccttggctg cgaccgcggc
     1441 ttcgcgacgt cgcggctcgg aggcgatatc gaggccgacg gcggcgcggt tgggcgcgac
     1501 cggttcgatg tactggatga cgaaacgctc gcccggattg gggcccagtt cccggatatg
     1561 gaagtctggc cagccatcgg cacgcgccgc cgcaagaaac tgctgttcgt cttcggggcg
     1621 tatccgccga atgatgccca tgccccgggc tcccggaaac tctcgagaga tgtctctcgt
     1681 cgccgcgtag gcgttgtagc gctcacgtcc gacctgtggg tgcccggcga cgaggaccgt
     1741 ccccgctgcg ccccgcagcc cgtattcgta ggtgtgcaag cgctgcaaga ggcggttcgc
     1801 catctcgtcg gtgatggtac gaaaccgctc atcgagagcg cgtgcgttct gttctgcttg
     1861 atgtttcgcg agtgcagcgc tcagcgcgac tccgagcagt aggaccgcca gtgcgactcg
     1921 cgccgcggtg gcgctcttca ggtatcggtt cttggacatg gcccgccctc accctcgcgc
     1981 acctcgacgc catggagagc ggacgaggac gttcgaggaa tgtcgatcga acaagcccgc
     2041 gcgcgacccg acgccttcgc ctggtagaga gcgcgatcgg cggcttggag cagcagtctc
     2101 gacgaatact gggctatttt cgcatttcgc agctcgctgc cttgctgcgg ctcacccaag
     2161 ttctcgttgg tcgctacacc aacgctcacc gtgacgacgc gggccgtggg cgaggcccca
     2221 tgtggaacgg ctagctccgc catggcggag aggacgcgct cggcaacgtg cagcgcgccg
     2281 acgttcggcg tttcgggcag tagcagcgcg aactcctcac ccccatagcg agccaccgtg
     2341 tcggctgggc gcagacagca cgacttcagc gcggtcgcga ccgcatgcag acaggtgtcg
     2401 ccctcggggt gcccgtacgt atcgttgaac agtttgaaat ggtcgacgtc caccaacagg
     2461 agcgccaaag gcagcttcga gcgccgcgat ctccgccact cgatttcgag cgtctcttcg
     2521 aagcggcgcc ggttggcgag ccccgtcagg gcatcggtcg tcgcgctgag ccgcagcgca
     2581 tccgccattc gcttgttccg cagctgcgca cgaaccctcg cgagcacgag ctttgggttc
     2641 accggcttgg cgatgaagtc caccgcgcct acatcgaacc ccttgacctc gaagctctcg
     2701 tcgcggtgcg acgtcacgaa gatgaccggc acctcttcca agccgggcgt gcgcttgagc
     2761 tccgtgcaca cctggaagcc gttcatgtcg ggcagctcgg cgtcgagcaa caccagatct
     2821 ggagctttgg tactcgccaa acgcaccgcg tcggcgcctt tggtcgcgaa acgcaccgta
     2881 ccctcccccg ccaggatgcg acccaggagc tggatcgcgc tgggatcgtc gtcgacgagc
     2941 aggatatctg cgctcacggc cgtcaaacgg cgaggccggg cgcccgttaa ctaccggaca
     3001 gcgccagaac gccacatgtg tcattaggcg agtttgtacg agttgctctg gccttaactc
     3061 gagcatcgat agtaagggac cgcccgccgc tgccggactg ccgacagagt gtcgcgtcgg
     3121 caaccgcggg ccaaagactc gaagtcccgc gttgggccgt agttacgaac tcgcttgcac
     3181 cggctctggc ttctcgaggg cgactgcgcc atccgcgggg cggatgcgac cgccgaaacg
     3241 gcgcgcccag gcctgcgtga actcggcgcc caag
//
LOCUS       DASTKS010000473         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1190628, whole genome shotgun sequence.
ACCESSION   DASTKS010000473 DASTKS010000000
VERSION     DASTKS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190628"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFQ35_09840"
     CDS             <1..884
                     /locus_tag="VFQ35_09840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9930979.1"
                     /translation="GTGKELVARAIHAWSGRTGPFYAINCATLSASIADAELFGYRRG
                     AFTGAGQGHLGHFRAAHGGTLFLDEIAELSLESQAKLLRALEQREVLPIGEHRAVAVD
                     VRVVCASQTELERAVAQGQFRADLRARLSGHTCVLPALRERREDVPALFLALLGRYTA
                     GRVPALSAKLVEALCLYSWPNNVRELELLARSLTALHADEARLGREFLPENIRGAPLF
                     QPAPARREAESTRENYELTELARVLHSTGGNVARAAAELGLSRQRLYRLMDRRPVREF
                     MNEYSSQQASTKAADAE"
     gene            835..2271
                     /locus_tag="VFQ35_09845"
     CDS             835..2271
                     /locus_tag="VFQ35_09845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9930980.1"
                     /translation="MSIAPSKHPLKPPTPSDDSGIEASEDTFADDRDRSGETLEGRYE
                     LLRKIGKGGMGEVYEARHLQLGRVFAVKLLLREHLSSKSMRQRFVREIRAASSLESMH
                     IVSALDCGHASDGAPYYVMELLRGESLGQLVRDQGRLSVPRALALAVQLCRGLSVAHS
                     RGLVHRDLKPENVFVVRDDSDRELAKILDFGLVRVDGGASLARAGSLIGTVRYMAPEQ
                     AADASRVDARADIYALGAILYESLTGCRPHPGDTAEEVLFHTLNSEPEPPSRLRPSLP
                     REFDAVVLRALERDPAHRFTTAFDFAAALERLSNSVAAASSARREPPISSTSITRDED
                     SALAPTKTSRRERWRWTVMLLTLLGSSGLMLCRRSPQPERASNGTRPPPVEAQREPPP
                     SPSVMSRGELTPHAPADSAVTPMPREASKLSNKKATAPRKRSSAPVSASTLASTNQAP
                     ATNADDASPATVFDSENPYLRTPAPAGP"
     gene            2397..3305
                     /locus_tag="VFQ35_09850"
     CDS             2397..3305
                     /locus_tag="VFQ35_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930981.1"
                     /translation="MHARARELDEAIREFREAYRIKPHYSVLYNLGQALSLAGKPVEA
                     TDTLTRYLHDGADAIAADRRASVEATLASLRRQVGELAFQVEPASAMLEVDGKSMDAA
                     ERAVPLRLSAGKHRWVARAGGHESSAGAVVVQGERRTEIEIRLAPSERAPLPPPAARK
                     VESGAPAPPGQPGPATTFADHDGSKAPQAIYIVGAVGASFGIAAGVLGIITAGRYADW
                     KRDRSALAEPQSAEDFREHQRQLTEDATSVRRLNTWTIGCATAGTALLAASATFWLLG
                     RSESERRGLRLSVTGTAASAEWRAEW"
     gene            complement(3424..3837)
                     /locus_tag="VFQ35_09855"
     CDS             complement(3424..3837)
                     /locus_tag="VFQ35_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930982.1"
                     /translation="MQAIPEIEESHWVETEPVPAPPSLVPALLSAPLLAPLFAPPLFA
                     PPLLAPPLLAPPLFVPPVTRPSMPAAPASAIDIARVPALPPHDVPAPCEPPCDAEAPP
                     PAMNVIPADPAESAPAMASCSPPVVAVPPNAPPPV"
     gene            3827..4570
                     /locus_tag="VFQ35_09860"
     CDS             3827..4570
                     /locus_tag="VFQ35_09860"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET9930983.1"
                     /translation="MACIPGATFTFSEDDPAGTKTRVATVKTFYLDLTEVTFGAFTEC
                     VEQGRCSPPDPPSTEEPSCVWGTDAPADYPVNCVTWFAARDYCLWRHKRLPTEEEWDY
                     AARRGTTANPWGDAPATHAQLNLLGDEWTGGASAFGNDGFVYLAPVGSYPLGATPEGI
                     QDLGGNVWEFTESKYCPYPQSPCSSCPLDEVCDNPCDACPDLPHQWVHRGGGWADYPD
                     ANRRWLRWRGYAVGGGRSGVVGFRCAKSR"
BASE COUNT          817 a         1556 c         1567 g          791 t
ORIGIN      
        1 ctggcaccgg taaggagctc gtcgcgcgag caatccacgc ctggagcggg aggacggggc
       61 ccttctacgc catcaactgc gcgaccctca gcgcgtctat cgccgacgca gagctcttcg
      121 gctaccgccg aggggcattc acgggcgccg gccagggcca cttgggccac tttcgagctg
      181 cgcatggggg cactttgttc ctcgacgaga tcgcggagtt gagtctcgag tctcaagcca
      241 agctcttgcg cgcactcgag cagcgagagg tgctgccaat tggggagcac cgggcggttg
      301 cggtcgatgt gcgtgtggtg tgcgccagcc agaccgagct cgagcgcgcc gtcgcgcagg
      361 ggcagttccg tgctgacctg cgcgcacggt tgagcggaca cacttgtgtc ttgcccgcct
      421 tacgcgagcg acgcgaggac gttcctgcgc tcttcctggc cttgctcggg cgctacacgg
      481 cgggtcgggt gcccgccctc agcgcgaagc tcgtagaagc gttgtgcctc tactcgtggc
      541 cgaacaacgt ccgcgagctc gagctcttgg cgcgctcgtt gaccgcgctg cacgctgacg
      601 aagcacgtct cggccgcgag tttctgccgg agaacatccg cggcgctccg ttgttccaac
      661 cggcgccggc gcggcgagaa gccgagagca cccgggaaaa ttacgagctc actgagcttg
      721 ctcgcgtgct gcacagcacc ggcggtaacg tggcgcgcgc cgcggccgag ctagggctct
      781 cgcggcagcg gctgtatcgc ctcatggatc gacgccccgt cagggagttc atgaatgagt
      841 atagctccca gcaagcatcc actaaagccg ccgacgccga gtgacgatag cggaatagag
      901 gcgagcgaag acacattcgc tgacgaccgc gaccggagcg gagagacgct cgagggccga
      961 tacgagctat tgcgcaagat cggcaagggc ggcatgggcg aagtatacga ggccaggcac
     1021 cttcagctcg gccgcgtctt cgcggtgaag ctcctgcttc gcgagcatct ttctagcaag
     1081 tcgatgcggc agcgtttcgt gcgtgagatt cgcgccgcga gttccttgga aagtatgcac
     1141 atcgtctcgg ctctcgattg cgggcacgcg agcgacggag cgccctacta cgtaatggaa
     1201 ctcttgcgcg gcgagtcgct tgggcagctc gtccgcgatc agggccggct ctccgtgccg
     1261 cgggcgctgg cgctcgcagt tcaactctgt cgcggtctct ccgtcgctca ttcacgcggg
     1321 ttggtgcacc gcgacctgaa gcccgagaat gttttcgttg tgcgggacga tagcgaccgg
     1381 gagctagcca aaatcctcga ctttgggttg gtgcgcgtcg acggcggagc gtcgctcgca
     1441 cgcgctggtt cgctgatagg aaccgtgcgc tatatggcgc ccgagcaggc cgcggacgcg
     1501 tcccgagtcg atgcacgggc ggacatctac gcactcggag ccattctcta cgagtctctg
     1561 acgggttgcc gcccgcaccc aggagacacg gctgaagagg tcttgttcca cacgctgaac
     1621 tcggaaccgg aaccccccag ccggctccgg ccaagcttgc cgagggagtt cgacgcagtc
     1681 gtgctgcgcg ctctcgagcg cgaccccgcc caccggttca cgaccgcgtt cgacttcgcc
     1741 gcggctctag aacggctctc gaactcggtg gccgcggcgt ccagcgctcg gcgcgagccg
     1801 cccatcagct cgacttcgat cacacgagac gaagactcgg ctctcgcgcc gacgaaaacg
     1861 tcacgccgcg agcgctggcg ctggaccgtc atgttgctca cccttctcgg ctcgagcggt
     1921 ctcatgcttt gccgacggag tccacagccc gaacgagcga gcaacgggac cagacccccg
     1981 cccgtcgagg cacaacgcga acctccgcct tcaccgagcg taatgagccg gggtgagctg
     2041 acaccccacg ccccagcgga tagcgcggtg acgccgatgc cgcgcgaggc gagcaaactt
     2101 tcgaacaaga aggcgaccgc cccgcgcaag cgatcgagcg cgccggtctc ggcgtcgacc
     2161 ttagcctcga cgaaccaagc gccagccacc aacgcagacg atgcgtcgcc cgctacggtg
     2221 ttcgactcgg agaacccgta tttgcggacg cctgctccgg cggggccgtg atgcgtcggc
     2281 tcgcgctggc cgtgcttacg gggtctgctt tttgctgtgt cgcgccccaa agctcggccg
     2341 acccaactac agatgacgcg accaccgaag ctgcgcggca ctacgagagt ggcgtcgtgc
     2401 acgctcgcgc ccgcgagctc gacgaggcaa tccgcgaatt tcgcgaggct taccggatca
     2461 aaccgcatta ctcggtactc tacaatctcg gtcaggcgct atcgctcgcg ggaaaaccgg
     2521 tagaagccac agacacactg acgcgctatt tgcacgacgg cgcggacgcc atcgccgcgg
     2581 atcgccgcgc gagcgtggag gcgacgctcg cttcgcttcg acgccaggtc ggcgaactcg
     2641 cgtttcaggt ggagcccgct tcggcgatgc tcgaagtcga tggcaagtcg atggacgctg
     2701 cggagcgagc cgtaccactt cgcctctcgg cggggaaaca ccgctgggtc gcgcgcgccg
     2761 gcggacacga gtcatcggca ggtgcggtcg tcgtccaagg tgaacgccgc accgagatag
     2821 aaattcgact tgctcccagc gagagagcgc cccttccccc acccgccgcg cgcaaggtcg
     2881 agtcgggcgc accggctcca cccggtcagc cgggaccggc gacgacgttc gccgaccacg
     2941 acggctcgaa ggctccgcag gcgatataca tcgtgggcgc ggtcggggcc tcgttcggta
     3001 tcgccgcggg cgtcctcggc atcatcaccg cgggccgtta tgcggattgg aagcgcgacc
     3061 gaagtgcgct tgcggaaccg caatcggccg aagacttccg cgagcaccaa cggcaactca
     3121 cggaggacgc gacgagcgtg cgacgcctca acacgtggac gatcgggtgt gccaccgccg
     3181 ggacagcgtt gttggccgcg agtgccacct tctggcttct cggcaggtca gagtccgaac
     3241 gtcgagggct tcgcctttcg gttaccggaa ccgccgcgtc tgccgagtgg agagccgagt
     3301 ggtaagacgt cccgtttgga tcttttgcct gtgcgctgca agctcgagct gccttctcga
     3361 cagctgctac gatgtctctg gagacgtggg gtggacgccc cttgccagcg gcggagctct
     3421 gaatcacaca gggggcggcg cgttcggggg aaccgccacg acaggcggag agcacgacgc
     3481 catggctggc gcggactccg ccggatctgc cggaatgacg ttcattgctg gtggcggcgc
     3541 ctccgcatca cacggaggct cgcaaggggc aggcacgtcg tggggcggca gcgccggtac
     3601 gcgagcgata tcgatggccg acgccggcgc ggcgggcatc gatggacgcg tgaccggcgg
     3661 aacgaacagc ggtggtgcga gcaacggtgg tgcgagcaac ggtggtgcga acaacggtgg
     3721 tgcgaacaac ggtgcgagca gcggcgcgct cagcagcgcc ggaaccaggg acggtggagc
     3781 aggcaccggc tcggtctcga cccaatgcga ttcttcgatt tctgggatgg cttgcattcc
     3841 tggcgccacc ttcacgttca gcgaggacga ccctgctggc accaagactc gcgttgcgac
     3901 cgtcaaaacc ttttatctcg acctcaccga agtgaccttc ggcgcgttca ccgagtgtgt
     3961 cgagcaaggt cgctgctcac cccccgaccc gccttccaca gaagagccct cgtgcgtttg
     4021 gggcactgat gctccagcgg actacccggt gaactgcgtg acgtggttcg cggcccgcga
     4081 ctattgtctg tggcgtcaca agcgtttgcc gaccgaagaa gaatgggact acgccgcgcg
     4141 ccgtggcacc actgcaaacc cttggggtga tgcgccggca acgcacgcgc aactgaacct
     4201 cctcggcgac gagtggactg gtggcgcaag tgccttcggt aatgatgggt tcgtatatct
     4261 ggcccctgtc ggcagctacc ccctcggggc gacgcccgag ggcattcagg atctcggggg
     4321 caatgtttgg gaattcaccg aatcaaaata ctgtccttat ccacagtccc cttgcagcag
     4381 ctgccccctc gacgaagtct gcgacaaccc gtgcgacgcc tgcccggatc ttccgcatca
     4441 atgggtacat cgaggcgggg gttgggcaga ttacccggac gcgaacaggc gctggctccg
     4501 atggcgaggg tacgccgtag gcggcggacg atcgggagtc gttggctttc gctgcgccaa
     4561 gtcacgctga ccgatgtcac cgtcacgggc gccgaggagt cgcctcgctt cgcaggctta
     4621 gcgcccgcga atggacaaat catactgcgc gggccgatcc ccttgctgcg ccgggcggtc
     4681 tcggctcgct cgcgactgac ttgtccattc gcgggctcca tgctcacgcc c
//
LOCUS       DASTKS010000474         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_522202, whole genome shotgun sequence.
ACCESSION   DASTKS010000474 DASTKS010000000
VERSION     DASTKS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522202"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFQ35_09865"
     CDS             <1..767
                     /locus_tag="VFQ35_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930984.1"
                     /translation="GGGVGFTAGGGAERAKGADAAIAGAQDLVKDIAKANAEVQKLAD
                     TLKSAKEKLSKGAFPEEEVSKLGSINIPFTGKNLSGKGIGRFKPEVVAQLIEYANGTT
                     AANEQKEKVQNVLTGAKKGIVELLEQQSKPQVRWAALLTGGPGGPWAGMQMIPAPFGA
                     KDKWPDEVKVNDKSFKRYQSGNPIREDSPFFVPVDPGSQTSVCPSDTIFKLRRELNDL
                     EEVLRGNNTPGQDRAGLIDQAQKLTVQLNKIGQAAP"
     gene            complement(954..2264)
                     /locus_tag="VFQ35_09870"
     CDS             complement(954..2264)
                     /locus_tag="VFQ35_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9930985.1"
                     /translation="MDSVPPSTLGGILVACLPAILAAAFAAASSAIGALSAARRAALR
                     DALEGSARSALDRYLAQGPAIESRWLVLRALGVALTALLVYARLPALWGVWSGLTAGS
                     VAVVAYALPSEIARALASRDPERAAPLLLKILHPVELLATPVSAPVFWLSRLVGGLLS
                     PAPTRAPGVTETEVEMIVTEGELNGSLAHEQSEMIRNVLEFGDVTAADVMVPRTQVTA
                     FEVTTPLQDVLKQITESGHSRYPVYAGSIDNVTGLLYAKDLLRHASDLGGRTLEGLLR
                     TPVAFVPEGQTGSSVLKDMRAGRQHMAIVIDEFGGTSGVLTLEDLLEKIVGDIRDEHD
                     VEEPPIQDLGNGRIVVDASVPIADLSRYLGAELPSDGDYHSLGGFISAQLGRVPEIGA
                     QLSAYGLHFEVREADERRVSTVEITRLAPPESIVPRSASRITAA"
     gene            2615..2977
                     /locus_tag="VFQ35_09875"
     CDS             2615..2977
                     /locus_tag="VFQ35_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930986.1"
                     /translation="MPTEGDPLDRSGHCFDPLALLVNPVALQAPAPPQEVSPSPVWVS
                     ETIRRIAWGGDRQRGTARVELGAGPYAGSALQVHAEGGALSLEITAPPGVDAGALAAR
                     IGARLEERGLRIESVRVR"
     gene            complement(3023..>3688)
                     /locus_tag="VFQ35_09880"
     CDS             complement(3023..>3688)
                     /locus_tag="VFQ35_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930987.1"
                     /translation="VARAGSREKRAFFVEGELRFVSSTSRDELLGHRLLRRAVITDAV
                     LKPALETRARCALHLGEILIGLGAIGPTALLRELIDQIEERFVDLLTWREGSLLYFPH
                     VKSGEEEVRSETAAPALVTRGVREGYSADELAMLLGPLARSLVLRGEARGLEPSRLGV
                     SEAEHALIERAISSGTLERLVAVAGQEGLGSVEDTLRAAFIGLSSSLIAAPSSGFSTS
                     GSS"
BASE COUNT          668 a         1206 c         1240 g          574 t
ORIGIN      
        1 tgggtggcgg cgttggcttc accgcgggtg gcggcgccga acgcgcgaag ggcgccgacg
       61 cggcgatcgc gggcgctcag gacctggtga aggatatcgc caaggcgaac gccgaagttc
      121 agaagcttgc cgacacgctc aagagcgcga aggagaagct ttcgaagggc gcgttccccg
      181 aggaagaggt ctcgaagctc ggtagcatca acattccgtt caccggcaag aacctctccg
      241 gcaagggcat cggccgcttc aagcccgagg tcgtcgcgca gctcatcgag tacgcgaacg
      301 gcaccaccgc tgcgaacgag cagaaggaga aggtgcagaa cgtcctcacc ggcgcgaaga
      361 agggcatcgt cgagctgctc gagcagcaga gcaagccgca agttcgttgg gcagcactgc
      421 tcacgggcgg cccgggtggt ccctgggcgg gtatgcagat gatcccggct ccgttcggcg
      481 ccaaagacaa gtggcctgac gaagtcaaag tcaacgacaa gtccttcaag cgctatcagt
      541 cgggtaaccc catccgagag gactcgccct tcttcgtccc ggtcgacccc ggctcacaga
      601 cctctgtctg tccgtcggac acgatcttca agctccgtcg cgagctgaac gacctcgaag
      661 aggtgttgcg cggcaacaac acgccgggtc aagatcgcgc cggcctcatc gatcaggcgc
      721 agaagctcac ggtccagctc aacaagatcg gccaagcggc gccgtaaccc ggctccctcg
      781 agcgacgacg acgagctcgg ttcccccgcg gaaccgggct cgttcttttt ttggggtgac
      841 cttcgcggga ttccgtttgt tcgccaggct cctttcggaa ctgcgctccc cccaaaaaat
      901 ctcggcgccc tccgcccccc tcggcgcctt ggcgttgaaa gcgcccggcg cctttacgca
      961 gccgtgatgc gggaagccga gcgggggacg atgctttcgg gtggggctag gcgcgtgatt
     1021 tcgacggtcg agacgcggcg ctcgtcggct tcacgcacct cgaagtgcag gccatacgcg
     1081 gagagctggg cgccgatctc gggtacgcga ccgagctgtg cgctgatgaa gccaccgagc
     1141 gagtgatagt caccgtcgga ggggagctcg gcgccgaggt agcggctcag atccgcgatg
     1201 gggacgctgg cgtcgacgac gatgcgcccg ttgccgagat cttggatcgg gggctcttcg
     1261 acgtcgtgct cgtcgcggat gtctccgacg atcttctcga gcagatcctc gagggtgagc
     1321 acaccgctcg tgccgccgaa ctcgtcgatg acgatcgcca tgtgctgacg gccagcgcgc
     1381 atgtctttca gcacgctgct gccggtctgc ccttctggca cgaacgcgac cggagtgcgg
     1441 agcaagccct cgagggtacg gccgccaagg tcgctcgcgt ggcgcagcaa atctttcgcg
     1501 tagagcaagc cggtcacgtt gtcgatgctg ccggcgtaca cggggtagcg cgaatggccg
     1561 ctctcggtga tctgcttgag cacgtcttgg aggggggtcg tgacctcgaa tgcagtgact
     1621 tgagtgcgcg gaaccatcac gtccgctgcc gtcacatcgc cgaactcgag gacgttgcgg
     1681 atcatctcgg actgctcgtg agcgagcgag ccgttcagct ctccctccgt cacgatcatc
     1741 tcgacttccg tctcggtgac tcccggagcg cgcgtcggcg ctggcgaaag caagccgccg
     1801 accaggcgac tcagccagaa cacaggagcg gacaccggcg ttgccagcag ctcgactgga
     1861 tgcaggatct tgagaaggag gggggcagcg cgctccggat cgcgagacgc cagcgctcgt
     1921 gcgatctcgc tcgggagcgc gtacgcgacg accgcgaccg atccggctgt caaaccggac
     1981 cacactcccc aaagcgcggg taggcgggcg taaaccaaga gcgccgtgag cgccacaccc
     2041 agcgcgcgca gcacgagcca gcgcgactcg atggcagggc cctgggcgag gtaccggtct
     2101 agcgccgagc gcgcgctccc ctcgagcgcg tcacgcaaag cagcgcgacg tgctgccgag
     2161 agcgcaccga ttgcggagct cgctgcagcg aacgcggctg ccaaaatggc agggagacaa
     2221 gcgacaagga tgccgccgag tgtagatggt gggactgaat ccaacggttc ctttggggcg
     2281 ccgggtcaaa gcaccacggc ccacttcaaa aagcctagcg gagccctcga tccccgcgca
     2341 accgaccgag tccccgaccg atgggggagt tgccatgcaa atagctgctc cgggctcgtc
     2401 gtcgccgcaa agaaaccaat cgatcgggtc gctcgttact gcaaaaagtc caacgatttc
     2461 agccagattt agcgctctac tcaccgcttc ggtccccagc gcacccagtg agcggccggt
     2521 cggtgcactc agcgaagggg gtctgaaaag aatcgcaccc tatcctactc atcggtgcca
     2581 ctcggagggg cttgcccgac aaaccaaaaa cggcgtgccg actgaagggg atccgctcga
     2641 tcgctccggg cactgcttcg atccgctcgc actgctagtg aacccggtgg ctctccaggc
     2701 gcccgcgccg ccgcaggaag tatcgccgtc accggtttgg gtgagcgaga ccattcgccg
     2761 catcgcttgg ggaggtgatc gacagcgcgg aacggcgcgg gtcgaactcg gcgcgggacc
     2821 gtacgccggc agcgcgcttc aggtgcacgc cgagggggga gcgctcagcc tcgagatcac
     2881 ggcgcctccc ggcgtcgacg cgggcgcgct cgctgcacgc atcggcgcgc gcctcgaaga
     2941 gcgcggcttg cgcatcgagt cggtgcgcgt gcgctgacgg tgcggatgcc tgagtggctg
     3001 ccgccgagcg ctggctatgc gatcaactgg agccgctcgt gctgaagccg gagctcggtg
     3061 ccgcaatcag cgacgacgaa aggccgatga aggccgcgcg gagcgtgtct tctacgctgc
     3121 cgaggccttc ttgtcccgcg acggctacga gccgttcgag cgtgcctgac gagatggctc
     3181 gctcgatgag agcgtgctcg gcctcgctga cgccgagccg gctcggctcg agcccgcgcg
     3241 cctcgccccg cagaaccagc gagcgtgcga gggggccgag cagcatggcg agctcatcgg
     3301 ctgaatatcc ctcgcggacg ccgcgtgtca ccagggcagg ggctgcggtt tcgcttcgca
     3361 cctcctcctc gccgctcttc acgtgcggga agtagaggag cgagccctcg cgccaggtga
     3421 gcagatcgac gaagcgctcc tcgatctgat cgatgagctc tctcagcagc gccgttggcc
     3481 cgatggcgcc aaggccgatc aggatttcgc cgaggtgcag cgcgcagcgg gcacgcgtct
     3541 cgagcgccgg cttgaggacg gcatcggtga tgacggcgcg ccgcagcaaa cggtgtccga
     3601 gcagctcgtc gcgggacgtg ctcgacacga agcgcagctc accctctacg aaaaatgcgc
     3661 gcttctcgcg ggaaccggcg cgcgccac
//
LOCUS       DASTKS010000475         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_967767, whole genome shotgun sequence.
ACCESSION   DASTKS010000475 DASTKS010000000
VERSION     DASTKS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967767"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFQ35_09885"
     CDS             complement(<1..497)
                     /locus_tag="VFQ35_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9930988.1"
                     /translation="MIDRTPSETFGRAYAAALGTGPSRSTLNAQRAALQGLVARYDQE
                     RRAALFRRVAPWAVGGSALAVAAAFALWVRLAPHAVQATLAGRTVAESAELAAREAQE
                     LSFSDGSDIVLAPSTRAVLTRATSDRAELRLNEGHLSASIRKKTGITWTIGAGPYSVQ
                     VVGTK"
     gene            complement(494..1141)
                     /locus_tag="VFQ35_09890"
     CDS             complement(494..1141)
                     /locus_tag="VFQ35_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9930989.1"
                     /translation="MSLHSIPSNLFEPGSAASDAESEAARRDEELASRLRDDPVAAMA
                     DLHARFAADVNRLVWRLLGADPDHNDIVQQVFCKIMLNGNRLRDLTRLGGWVQSITVN
                     TVYEELRRRDVRRVFQRDANDVSFHPDLVRDVEVRDLLLRAKRIIDKLPAKERIVFVL
                     HFVEERPLPEVAELCEYSLATAKRRLNAANKRFAALAGKNSDLLRLFERRGEGER"
     gene            1464..2078
                     /locus_tag="VFQ35_09895"
     CDS             1464..2078
                     /locus_tag="VFQ35_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9930990.1"
                     /translation="MSDADTVDVNDAVLRRALLRRERWAAAAVWKRFSPRVFSIFSRS
                     LPRSKASEELTQEVMLHALDEASRARRVKCLGSLVYGQAARVLRREFRRDAFRRRYWP
                     RSRAKAAELVLVADRTERAALLKLYDALDQLSAADRVLFVLHEFELLSDVEIADALDL
                     PLLKTRKRLARALGRIESEVDCDPLLAAYLCDDTSLDRDLQEGA"
     gene            2075..>2429
                     /locus_tag="VFQ35_09900"
     CDS             2075..>2429
                     /locus_tag="VFQ35_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930991.1"
                     /translation="MSALSRGRYDFRGLVRRAHPEMTPEQESSGWHMLRQRGLLRERR
                     ARRWRVAVFGVGVALGSGVLGYVCARPPRPPTLEYSVSGAHVGAGGTLRVDAEKGGYL
                     RFSNGSVVRLARNANA"
BASE COUNT          393 a          769 c          819 g          448 t
ORIGIN      
        1 aacttggtgc caactacctg aaccgagtac gggccggcgc cgatagtcca ggtgatgccc
       61 gtcttcttac ggatcgacgc cgacaggtgg ccctcgttga gtcgcagctc ggctcggtcg
      121 ctcgttgcgc gggtgagcac ggcgcgcgtc gacggcgcga gcacgatgtc gctgccgtcg
      181 gaaaacgaga gttcctgcgc ctcgcgcgcg gcaagctcgg cggactcggc tacggttcgt
      241 cctgcgagcg ttgcttgcac cgcgtgcggc gcaagcctca cccagagcgc gaaggcggcc
      301 gcaaccgcca gcgcactacc gccgacagcc cagggcgcaa ctcgacgaaa cagcgccgcg
      361 cgcctctcct ggtcgtagcg agccacgagc ccttgcaacg cggctcgttg ggcgttcagc
      421 gtgctgcgcg aggggccggt gcccagggcc gctgcgtagg ctcgcccaaa ggtctcgctc
      481 ggggtgcggt cgatcatcgt tctccttctc cccgacgttc gaagagcctc aacaggtccg
      541 agttttttcc agccagcgct gcgaagcgct tgttggcggc gttcaggcgc cgctttgcag
      601 tggcgaggga gtattcgcag agctccgcca cctccggcag agggcgttct tccacgaaat
      661 gcagcacgaa cacgatgcgc tccttggccg gtagcttatc gatgatgcgc ttggcgcgca
      721 ggagtagatc tctcacttcg acgtcgcgaa cgagatcggg atgaaacgac acatcattcg
      781 cgtcgcgctg aaacacgcgt cggacgtcgc gtcggcgaag ctcttcgtaa accgtgttaa
      841 ccgtgatcga ctgcacccaa ccaccgagcc gcgtgagatc gcgtagacgg ttgccgttga
      901 gcatgatctt gcagaaaacc tgctgaacga tgtcgttgtg atccggatcc gctccgagca
      961 gacgccacac caagcggttc acgtccgcag cgaaccgcgc atgaagatcc gccatggccg
     1021 caaccggatc gtcgcgcagt cgcgaggcga gctcttcgtc gcggcgcgca gcctccgatt
     1081 cggcgtcgct ggccgctgag ccgggctcga agagattgct tggtattgag tgaaggctca
     1141 tccgcggcgt taagtggccg gagggggcgt cacacacgca cccgactggg atcgaatatg
     1201 cctcccgagt cgcacgagtt ccagcgattc cggggtgggc cgcggccggc cacttccatg
     1261 gcgcccgctt taccgcgaaa agtaagggtt ctttcccttt tgacaaattt cgcgaaagtt
     1321 tgacaacaca ggggggccag cgggcactga atggatgaaa atgttccgtt cgttcggcat
     1381 tcggctgctc gcagccccgc gcacgaggag cgctggaggc gcacttggcc gctagcacga
     1441 gtcgtattcc ttacctccgg ctagtgagcg atgccgacac tgtcgacgtg aacgatgccg
     1501 tgctccgacg tgcgctcctg cggcgcgagc gctgggcggc ggcggcagtg tggaagcggt
     1561 tctcgccgcg ggttttttcg attttttcgc gtagcctgcc gcgctccaaa gcgagcgaag
     1621 agctgacgca agaggtcatg ctccacgcgc tggacgaggc ctctcgagca cgccgcgtga
     1681 agtgcctggg tagcttggtc tacgggcagg ccgctcgcgt gctgcgacga gagtttcgcc
     1741 gtgacgcgtt tcgccggcgg tactggccga ggtcgcgtgc gaaggcggcg gagctcgtcc
     1801 tcgtcgcgga tcgtacggag cgagccgcgc tactgaaact gtacgatgcg ctcgatcagc
     1861 tgagcgccgc ggatcgagtt ctcttcgtgc tgcacgagtt cgagctcttg tcggacgtcg
     1921 aaatcgccga tgccctcgac cttcctctgc tgaagacgcg caagcgtttg gcgcgcgcgc
     1981 tcggcagaat cgagagcgaa gtcgattgcg atccgctgct cgctgcatat ctctgcgacg
     2041 acacgtcgct cgatcgggat ttgcaggagg gggcgtgagc gccctttcgc gcgggcgata
     2101 cgactttcgg ggattggttc ggcgggcgca ccccgagatg acgccggagc aggagagctc
     2161 gggctggcac atgctgcgtc aacgcggttt gctgcgggag cggcgagcgc ggcgttggcg
     2221 cgtcgccgtg ttcggcgttg gcgtagcgct aggctccggc gttctcggat acgtctgcgc
     2281 ccgtccgccc cggccgccga cgctcgagta ctccgtcagc ggcgcgcacg tcggcgctgg
     2341 cggtactctc cgcgtcgacg ccgaaaaggg gggctacctg cgcttttcga acggctcggt
     2401 ggtgcgcttg gcgcgcaacg ccaacgccc
//
LOCUS       DASTKS010000476         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1191423, whole genome shotgun sequence.
ACCESSION   DASTKS010000476 DASTKS010000000
VERSION     DASTKS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191423"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFQ35_09905"
     CDS             complement(<1..710)
                     /locus_tag="VFQ35_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET9930992.1"
                     /translation="MSDSLSSDLAALRIDRGNTRRPGGGPLKTVLALLIAAGALAAGY
                     AFGKPYLEAKLFKTQVDVTEIALVSPAQASVELTATGYIVAQSVSKVSAKVPGKVAKV
                     FVKQSDLVKPGDVLFVIDPMDQNATIAAARSQAAAARARAVAARANQSEIEVQLKRAR
                     ALAAQGVGPQSSVDDLAARSTSLREQVNASDADAKASSALVSQLEVNLKNFTVVAPIG
                     GTVINRPPELGEFVGPQPA"
     gene            1039..1704
                     /locus_tag="VFQ35_09910"
     CDS             1039..1704
                     /locus_tag="VFQ35_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9930993.1"
                     /translation="MQFSQLAQIGVVIFAAVGVYGFVAAARDGEVRRDCSALCALKPN
                     YAARNRLAPDFELPALSGGKRRLSSYRGKTVILNFWTKNCRPCLEEMPSLADLARSLR
                     DHPNVVLLTVSTDDSLDDARDTLKSVLGGDAPFEVLIDPDSKVVADRYGTKLYPETWF
                     IDPKGVIRARFDGARDWASALSVDLAESLARPSPFSCSVEFDKGQLREDTLGVCEDPG
                     RGG"
     gene            1701..1976
                     /locus_tag="VFQ35_09915"
     CDS             1701..1976
                     /locus_tag="VFQ35_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9930994.1"
                     /translation="MKHIVCRCEDVTLEELERAVKLGHRDIESLKRYTGFGTGICQGK
                     SCLVACARRLVELGGDAGFGITPRPPYHPVSLAALAALDPDSLSERT"
     gene            complement(2023..2385)
                     /gene="queD"
                     /locus_tag="VFQ35_09920"
     CDS             complement(2023..2385)
                     /gene="queD"
                     /locus_tag="VFQ35_09920"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET9930995.1"
                     /translation="MNVRLIRELRFEAAHRLPKVPAGHKCARLHGHSFKIELAIVGPV
                     NPETGWLIDFAEIDRVWQPLHDQLDHNYLNEVPGLENPTSENLAHWLWNALKPALPEL
                     ERVIVHETCDARCEYEGK"
     gene            complement(2426..>5106)
                     /locus_tag="VFQ35_09925"
     CDS             complement(2426..>5106)
                     /locus_tag="VFQ35_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9930996.1"
                     /translation="LDFGKFPLGDIKNSKFKFRPLWVEITDAQARKGSSDFTVPTARI
                     DFGTRASLLVEARAKADSMDLRDFFAMWHFDEDPRFDPIRGRTGLDTSLRYILGGPED
                     HCGGGALRIAGKLKAHHLDMFDERYDGGDASFDFLWHDRDATYRGIVLSVPDLVLRKG
                     KGVLTGKLNVQTGAKLSGTGAALGVPLAKIDALPNALRAAEGTVNATATVSGTVDAME
                     LSASGTISPVKVGRVTLPASTFHTDLEPIRRSETPAGTTRCGQPISGPFDRNDYDRDL
                     ALGVFHTSGSFFGGQITTNDVSVTRQRNKVLHGDVSLNDADIGGLLELRPEVALTGVP
                     VNGHVTASIGIDELAMNDPASARGTLTLKKLSLDRNGYRAELLPGADPLALNARRLSV
                     PKAAFAITTPRGVKATIDVAAGLDRLGAGANVDATVDVRPTPLAGLVGLMPRVERADG
                     TVGGHVRLNGALGAPRVSGNFGIERGELVLRGLPTTISNIDVGLGFADGELTITRGNA
                     RIGNGTLQLSGGAPLNGLELGEARIRAVAREVALPMKDGVRATADADLTIVWKPPGSD
                     EEHNLPRVTGNALLRSFEYRRPVTIAADLNALAQRGKRTEFEAYDPSEDAVEFDVLLR
                     AQRSLRIQNDIVDTELVLSEDGLELAGTNARFGLRGALKLKPGGHVFLRRSDFEIREG
                     SVRFDDLTRIAARVDVTAVTEYRRYTSSLGSAGGGAAASGTGVTGGRWSIRMHAYGDA
                     DNLHVDLTSDPALPQDDIFLLLTVGLTRAELDQAQSASVGGSVALEALGTLTGADRAV
                     KNAVPIIDEFRFGSAYSSRTGRTEPTVTVGKRLTDRVRANVTSGLAESREVRSNVEWR
                     LSPRVSVEGSYDNVNDISSSSLGNLGADVRWRLEFE"
BASE COUNT          799 a         1677 c         1736 g          894 t
ORIGIN      
        1 gccggttgcg gccccacgaa ctcaccgagc tcgggcgggc gattgatcac cgtgccgccg
       61 atcggcgcga ccaccgtgaa gttcttcaag ttcacctcga gctgcgacac gagcgcgctc
      121 gacgccttcg cgtccgcgtc cgaggcgttg acctgctcac gcagcgacgt cgaccgcgcg
      181 gcgagatcgt cgacgctcga ttgcggtccc acgccctggg ccgccagcgc ccgagcgcgc
      241 ttgagctgca cttcgatttc gctctgattc gcgcgcgccg cgacggctct cgcgcgcgct
      301 gccgcggcct gactgcgggc ggccgcgatc gtcgcgttct gatccatcgg gtcgatcacg
      361 aacagcacgt cgccgggctt gacgaggtcg ctctgcttca cgaacacctt cgcgaccttg
      421 ccggggacct tcgcgctcac tttcgagacc gactgcgcga cgatgtatcc ggtcgcggtg
      481 agctcgaccg acgcctgcgc cggagaaacg agggcgatct cggtgacgtc gacttgggtc
      541 ttgaatagct tcgcttcgag gtagggtttt ccgaaggcgt atccagccgc gagcgcgccc
      601 gccgcgatca gcagcgcgag caccgttttg agcgggccgc cgcctggtcg acgagtgttg
      661 ccgcggtcga tgcgcagcgc agcaagatcg gatgacagtg aatcggacat gcgaagcctg
      721 aggtgacgag ccgccgactc gctagcacgg ccggaagcgc gcgtctttct ggcaccagcg
      781 cgcccccgag ggcgatggca gcttaggacc gctcgcgaat ttgccaagag caggcgtcag
      841 tttcaacgag cgttttttcg aaacacggag ccgcgggacg ctctcgcggg tttggtcccg
      901 aagagtcgct cgagcgtcca tgcgagatct cgtttattct cgaggggtga acggggccgt
      961 caccgatgcg ggtcgtcaaa accccagtga attcgattgt cgctgcccgc cgaccggccc
     1021 gagtgatagg ctcccggcgt gcagttttca cagctcgctc aaatcggcgt cgtcatcttc
     1081 gcggcggtag gtgtgtacgg tttcgtggcc gcagctcgcg atggcgaagt gcgccgtgat
     1141 tgttcggctt tgtgcgcgtt gaagccgaac tacgcggcgc gcaatcggct cgcgcccgac
     1201 ttcgagctcc ccgctctctc tggcggcaag cgccggctca gctcttatcg cggcaagacg
     1261 gtgatcttga acttttggac gaagaactgc cgcccttgct tggaagagat gccctcgctc
     1321 gcggatctgg cgcgctcgct gcgtgaccat cccaacgtcg ttctcctcac ggtttcgacc
     1381 gacgacagct tggacgacgc gcgcgacact ttgaagtccg tgctcggggg cgacgcgccg
     1441 ttcgaggtgc tgatcgatcc ggattcgaag gtcgtcgcag atcgctacgg gacgaagctc
     1501 tacccggaga cttggttcat cgacccgaag ggggtcattc gcgctcgctt cgacggcgct
     1561 cgtgattggg cgagcgcgct cagcgtagac ctcgccgaga gcctcgcgcg gccgtctccg
     1621 ttttcgtgca gcgtcgagtt cgacaaaggg cagctgcgcg aagacacgct cggcgtctgt
     1681 gaagacccgg gtcgcggcgg atgaagcaca tcgtgtgtcg ctgcgaggac gtcaccctcg
     1741 aagagctcga gcgcgcagta aagctcggtc accgcgacat cgaatcgctc aagcgctaca
     1801 ccgggttcgg taccgggatc tgccagggaa aatcgtgctt ggtcgcctgt gcacggcgcc
     1861 tggtcgagct cggtggcgac gcaggtttcg ggatcacccc gcggcctccg tatcaccccg
     1921 tctcgctagc ggcactcgcc gcgctcgacc cggattcact ctccgaaagg acgtgaccag
     1981 cgccggtctg ctcgcgacac cgcgagcgcc gtagccgcgc cgctacttac cctcgtactc
     2041 gcagcgcgcg tcgcacgttt cgtgaacgat gactcgctcg agctcgggca gggccggctt
     2101 gagcgcgttc caaagccagt gcgcgaggtt ctcactcgtc gggttttcga gcccgggtac
     2161 ctcgttcagg tagttgtggt cgagctggtc gtggagcggc tgccagacgc ggtcgatctc
     2221 cgcgaagtcg atgagccagc ccgtctccgg attcacgggg ccgacgatgg cgagctcgat
     2281 tttgaagctg tggccgtgca gccgagcgca cttgtggccg gcgggaacct tgggcaaccg
     2341 atgggcagcc tcgaagcgca actcacgaat caggcgaacg ttcacgcggc cgagcctagc
     2401 cccgaatcaa gagcctgcca aacgctcact cgaattcgag gcgccagcgc acgtccgcgc
     2461 cgagattccc gagagaagag ctcgagatgt cgttcacgtt atcgtacgag ccttcgacgc
     2521 tgacgcgcgg ggacaggcgc cactcgacgt tggacctgac ttcgcgcgac tcggcgaggc
     2581 cgctcgtcac gttcgcgcgc actctgtcgg tcaggcgctt tcccaccgtg acggtcggct
     2641 cggtgcggcc cgtgcgcgac gaatacgcgg agccaaagcg gaattcgtcg atgatgggca
     2701 ccgcgttctt cacggcgcga tcggcaccgg taagggtgcc gagcgcttcg agcgcgacgc
     2761 taccgccgac gctcgcgctc tgagcttggt cgagttcggc ccgcgtgagc ccgacggtga
     2821 gaagcaagaa gatgtcgtcc tgcggaagcg ccggatcgct cgtgagatcg acgtgcaggt
     2881 tgtcggcgtc tccgtaggcg tgcatgcgga tactccagcg cccaccggtc acgcccgtgc
     2941 cgctcgcggc cgctccgccg ccggcgctgc cgagcgacga agtgtagcgt cgatactcgg
     3001 tgaccgccgt cacgtcgacg cgcgcggcga tgcgcgtgag gtcgtcgaag cgcacgctgc
     3061 cttcgcgaat ttcgaagtcg ctgcggcgta agaagacgtg cccgcccggc ttcaatttga
     3121 gcgcgccgcg cagaccaaag cgcgcgttcg tgccggcgag ctcgaggccg tcttccgaga
     3181 ggacgagctc cgtgtcgacg atgtcgttct gaatccgcaa cgatcgctgc gcccgcaaca
     3241 gcacatcgaa ctccaccgcg tcctcgctcg gatcgtaggc ttcgaattcg gtgcgcttgc
     3301 cgcgctgagc gagcgcgttc aaatccgccg cgatcgtcac ggggcgccgg tactcgaagg
     3361 agcgcaagag agcgttgccg gtgacgcgcg gtaggttgtg ctcctcgtcg gaacccggcg
     3421 gcttccatac gatggtcaga tccgcatctg ccgtggcgcg cacgccgtcc ttcatcggaa
     3481 gcgcgacttc gcgagcgacc gcgcggatgc gggcttcgcc gagctcgagg ccattcaggg
     3541 gcgcgccgcc gctgagctgg agcgtgccgt tcccgatgcg cgcgttgccg cgagtgatgg
     3601 tgagctcgcc gtcggcgaag cccaatccga cgtcgatgtt gctgatcgtc gtgggtagcc
     3661 cgcgcagcac gagctcgccg cgctcgatgc cgaagttgcc cgagacgcgc ggcgctccga
     3721 gcgcgccgtt caagcgcacg tggccgccta cggtgccgtc ggcgcgctcg acgcgcggca
     3781 tgaggccgac caagccggcg agcggcgtgg ggcggacgtc gaccgtcgcg tccacgtttg
     3841 cgccagcgcc gagccgatcg agcccggcgg ccacgtcgat ggttgctttg acgccgcgcg
     3901 gggtcgtgat ggcgaaagcg gctttgggga cgctcagccg acgcgcgttg agcgcgagcg
     3961 gatcggcgcc gggcaagagc tcggcgcgat agccattgcg gtccaacgag agcttcttca
     4021 gcgtaagggt tccgcgcgcg ctcgcggggt cgttcatggc gagctcgtcg atgccgatgc
     4081 tcgcggtgac gtggccgttt acgggcacgc ccgtgagcgc gacttctggt cgaagctcga
     4141 gcaggccacc gatgtcggcg tcgttcaacg agacgtcgcc gtgcaagacc ttgttgcgct
     4201 gccgcgtgac gctgacgtcg ttggtcgtga tctgcccgcc gaagaacgag cccgaggtgt
     4261 ggaacacgcc gagcgcgagg tcgcggtcgt agtcgttgcg atcgaacggg cccgaaatcg
     4321 gctgtccgca gcgggtggta ccggcgggcg tttcgctccg gcggatgggc tcgaggtcgg
     4381 tgtggaacgt cgaagcgggc agcgttacac gccccacctt caccggtgaa atggtgccgc
     4441 tcgcgctgag ctccatcgcg tcgacggtgc cggacacggt cgcggtcgcg ttcacggtgc
     4501 cctcggccgc gcgcagcgcg ttcggcagcg cgtcgatctt cgcgagcgga acgccgagcg
     4561 cagcgccggt ccccgagagc ttcgcgccgg tctgcacgtt gagcttgccg gtgagcaccc
     4621 ccttgccctt gcgcagcacg agatccggaa cgctgagcac gatgccgcga tacgtggcgt
     4681 cgcggtcgtg ccacaagaag tcgaagctcg cgtctcctcc gtcatagcgc tcgtcgaaca
     4741 tgtcgaggtg atgagccttg agctttccgg cgatgcgcag cgcgccgccg ccgcaatggt
     4801 cctcgggccc gcccaggatg tagcgcagcg acgtatcgag ccccgtgcgc ccgcggatcg
     4861 ggtcgaagcg cggatcctcg tcgaagtgcc acatcgcgaa gaagtcgcgc agatccatgg
     4921 agtcggcctt ggcgcgcgcc tccacgagca gcgacgcgcg cgtaccgaag tcgatgcgcg
     4981 cggtcggcac cgtgaagtcg ctagacccct tgcgcgcctg cgcgtcggtg atctccaccc
     5041 acaacgggcg aaacttgaac ttcgagttct tgatgtcgcc gagcgggaac ttgccgaagt
     5101 ccaagt
//
LOCUS       DASTKS010000477         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_744512, whole genome shotgun sequence.
ACCESSION   DASTKS010000477 DASTKS010000000
VERSION     DASTKS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744512"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..805
                     /locus_tag="VFQ35_09930"
     CDS             170..805
                     /locus_tag="VFQ35_09930"
                     /inference="COORDINATES: protein motif:HMM:NF036522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9930997.1"
                     /translation="MPKPRPPVTSPRKAPVQKRSTQLVADILKAAVRVLEKEGAERFT
                     TIRVAKAAGVSVGSLYQYFPNKQAILYRLQTDEWEQTGRMVDAILSNSTETPAERLHT
                     MTRAFFRSECDEAPLRQALDRAAPSYHDAPESVVHRERGRKVIQAFIAEAAPRASARQ
                     RRLAADLLFMTVTAIGKQVSERRPSPVELEHWADAVAEMIATYLATLGAPG"
     gene            complement(836..1360)
                     /locus_tag="VFQ35_09935"
     CDS             complement(836..1360)
                     /locus_tag="VFQ35_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9930998.1"
                     /translation="MATWSKLSVCFGFCVLLVGACGDDDDTTASSGGAAGAGGAHTSG
                     GVGGKGNTGGSAGKGGGAPGGSGGASTGGETTSGGASSGGVGGNMSLAGGGAGGVSHA
                     VECRDDTQYIDDLANKACAVEDASCSFGLDCCCGTCHVFHFCRCENHEWTCWSEDWCR
                     DGCGGAGGGGGGNG"
     gene            1585..2223
                     /locus_tag="VFQ35_09940"
     CDS             1585..2223
                     /locus_tag="VFQ35_09940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9930999.1"
                     /translation="MNSRTAIFRMKPHRTMPVSEPALRPPKERNSGGVGEYRLHYLVQ
                     APSLISWSRLLFAALFPWAASTPWSALLVLALAGASDVLDGWVARRYGLVTATGAVVD
                     GITDKVFALVVVVTLLEQGRAPWASLALLGAREIGELPLVVWLAVSRAARHARTVNAK
                     ANVLGKAATVLQFVTVGAMLGGISRLGVLLGATALVGVLAAVSYWLRELGRD"
     gene            2338..>4352
                     /locus_tag="VFQ35_09945"
     CDS             2338..>4352
                     /locus_tag="VFQ35_09945"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9931000.1"
                     /translation="MRSRRLHCYGRTLVGAISITGLGACSSAGEQPVGDSALSDTAMD
                     VAPLSAEQVSAVGANLRGRSLNSVKLRELIARQVGGLEKLRVPADDASIPLPPEDPSR
                     PGRYATTEAKRYLGKLLFHDPIRTARINVNTNVNPPIRAGEPRDLPQGTSFGGTVPSS
                     NPNVQKVVDATESTASCGSCHLGEAAGKAGAVLNFAVGGEGRGYTDANGNYIVRRRPM
                     KDLIARESQYRPDLRLFEGDTGVDSLPTLTDIYRVAGSVEIATPARQKADPLPSALLA
                     TGRLDQLDSVARQSPSMVGFAFNNRLLLGGFAGESNSLPAGLNPLNDPAQENLTLLLL
                     DAHRMLDFESAKLQSIPAFVELFAQAFPEEARGYEECRAAGPNPTCQGELDKLVNDET
                     VLRATATFLRTAVTRNTAFDRFLAGDDSLTASQRRGAALFFTKAGEGGAGCFSCHSGP
                     MLNKQPNDPDVTGIGHFVEENFFNIGIGDHPLQALNAMRRGRLRFDADGKPLSHGEDT
                     GRQEITQNPDHAYKFRSLTLRQLKDARTFFHNGSFTNLRDVVSYFNRGIPQDREFAGA
                     ARTLEPRFTNPRGARAPRGLGLSDDDVDALTDFIENGLYDPTFVQSFQPNEADLNYSK
                     NHPELAALGAKDGQLLSGLAMDNDDALSRRDQGLEFLDVSQSVRNER"
BASE COUNT          798 a         1421 c         1359 g          774 t
ORIGIN      
        1 cctggtcgag gtgagcgacg cgctcgcgta gcttcgcatc actcgctttg gcttctagaa
       61 aaagtcggtt caagagctcg gcgacgcggg gggattcgag tgtgttggtc atcgtgctaa
      121 agaatacgaa taattattcg cgttcggtat tcgcgttgac ggtctgccca tgcccaagcc
      181 tcgcccgccg gttacttctc cgagaaaagc ccccgtccaa aagcgctcca cccaactcgt
      241 ggcggacatc ttgaaagcgg ctgttcgcgt tctggagaaa gaaggtgcag agcgtttcac
      301 caccatccgc gtcgcgaagg cggcgggcgt cagcgttggc tcgctctacc aatacttccc
      361 caacaaacag gcgatccttt accggctgca gacggacgag tgggagcaga cggggcgcat
      421 ggtcgacgca attctgagta actcgaccga gacgcctgcc gagcgcctgc acacgatgac
      481 gcgagcgttc ttccgctccg agtgcgacga agcgccattg cgccaggcgc tcgaccgagc
      541 tgcaccgagc taccacgatg cccccgagtc ggtcgtgcac cgcgagcgcg gccgtaaggt
      601 aattcaggca ttcatcgccg aagcggcgcc ccgcgcctcg gcgcgtcaac ggcgcttggc
      661 cgccgatctg ctcttcatga cggtgactgc catcggcaaa caagtctccg agcgtcggcc
      721 ctcaccggtc gagctcgagc actgggcgga cgcggtagcg gagatgatcg cgacgtacct
      781 ggcaaccctc ggcgcgccag gttgagcggt atgatggaac gaaagtgagg gtggcctacc
      841 cgttgccacc tccgccaccc ccggcccccc cgcagccgtc gcgacaccag tcttcggacc
      901 agcaagtcca ctcgtgattt tcgcatctac agaagtgaaa gacatggcaa gttccgcagc
      961 agcagtccaa gccgaaactg caggatgcgt cctcgactgc gcacgctttg ttcgcgaggt
     1021 catcgatata ctgggtgtcg tcgcggcact cgacggcgtg gctcactccg cctgcgccac
     1081 caccggcgag cgacatgttg ccgccgacgc cgccgctgct cgcgcctccg ctcgttgtct
     1141 cgcctccggt tgacgctcct cccgagccac ccggcgcccc tccacctttg ccggcactgc
     1201 ctccggtgtt tccctttcct ccgactcccc cgctcgtatg tgcgccgccg gcccctgctg
     1261 cgccaccgct cgaagccgtc gtgtcgtcat cgtcgccgca cgctccgacc aggagcacgc
     1321 aaaagccaaa gcacaccgat agtttgctcc atgttgccat acaacctcca tggacgattc
     1381 tctgtggacg ggctcggaag tcaaggttgc gattgtgccg tggcgcacga accgcgtcga
     1441 aaagtgcaaa ttgtgcgatt ggggagagcc gaaaaggcgt cgcccaacat tgccgactcg
     1501 tcgtgagcac gcggtgcgcc gccggcagag tcgaaattcg ataccgcgcg aagaattggc
     1561 gcgcgtgttg cgtctcccca tgtcatgaac tcgaggacgg cgatttttcg catgaagccg
     1621 caccgcacaa tgccggtctc ggagcccgcg ttgcgtccgc cgaaagagcg gaacagcgga
     1681 ggtgttggcg aatacaggct gcactactta gttcaggccc cctcgctcat cagctggtcg
     1741 cggctcctgt tcgcggcgct ctttccgtgg gctgcgagca cgccatggtc ggcgctgctg
     1801 gtcctcgcgc tcgctggtgc gagcgatgta ctcgatggtt gggtggcgcg tcgttatgga
     1861 ttggtcaccg caaccggtgc ggtcgtagac ggaatcaccg ataaagtgtt tgcgctcgtg
     1921 gtcgtcgtca cgttgctcga gcaggggcga gcgccgtggg cgtctctcgc tctcctcggt
     1981 gcgcgagaaa tcggcgaact tccactcgtc gtgtggctcg ccgtgagccg ggccgcccgg
     2041 cacgctcgca ccgtcaacgc caaagccaac gtgctcggta aggccgccac ggttcttcag
     2101 ttcgtgaccg tcggcgcgat gctcgggggg atctcgcgcc ttggagtgtt gctcggcgcg
     2161 accgcgctgg tgggggtttt ggcggccgtg agctattggc tccgcgagct cggccgcgat
     2221 tgactaccct ctcctagaat ttcgtccccg ccgaagtaac caggtgcgga tcgtcccgta
     2281 ccccgcgatg cgcgtacccc gcgtgaagcg aagttacgga aacggaggac gagactcatg
     2341 aggtcacgaa gattacactg ctatggccgc acgctggtcg gcgcgatttc aatcacaggg
     2401 ctcggcgcgt gttccagtgc gggcgagcag ccggtaggag actcggcact ctccgacacg
     2461 gcgatggacg tcgcgccgct gagcgccgaa caggtgtcgg cggtcggggc gaacctacgc
     2521 ggccgtagcc tcaattcggt caaattgcgc gagctcatcg cgcgccaggt cggtggactc
     2581 gagaagctca gagttccagc cgatgacgcg agcatcccgc tgcccccgga ggatccaagt
     2641 cgtccgggcc gttacgcgac gaccgaggcg aagcgatacc tcgggaagtt gctgttccac
     2701 gatccgatcc ggacggctcg catcaacgtg aacacgaacg taaatccccc gatccgcgcc
     2761 ggtgagccga gagacttgcc gcagggaacg tctttcggag gaacagtgcc gagctcgaat
     2821 ccgaacgtgc agaaggtcgt cgacgcgacg gagagcacgg catcctgtgg cagctgccat
     2881 ctcggcgagg ccgccggcaa agcaggtgcg gtgttgaact tcgcggtcgg cggcgagggc
     2941 cgcggctaca cggacgcaaa cggcaactac atcgtcagac gtcgcccgat gaaggacctc
     3001 atcgcgcgtg agagccaata caggcccgac cttcggctgt tcgagggcga caccggtgtc
     3061 gactcgctcc cgacgctcac ggatatttat cgggtcgccg gctcggtcga gatcgccacc
     3121 ccagctcgcc aaaaggcaga tccgcttccg agcgcgctcc tcgcgacggg gcgtctcgac
     3181 cagctcgata gcgtggcgcg gcagtcaccg tccatggtcg ggtttgcttt caacaaccgg
     3241 cttctcctcg gcggctttgc cggggagtcc aactccttgc cggcggggct gaatccgctg
     3301 aacgacccgg ctcaagagaa cctcactttg ctcctgctcg acgcgcaccg catgctggat
     3361 ttcgagtccg cgaagctcca gagcattccc gctttcgtcg agctcttcgc gcaagcattc
     3421 cccgaggagg cccgcggcta cgaagaatgc agagcagcgg gccccaaccc aacctgccag
     3481 ggagagctcg acaaactcgt gaacgacgag acggtgctgc gcgcgaccgc gacgttctta
     3541 cgcaccgcgg ttacgcgcaa cacggcgttc gatcgattcc tcgccggcga cgactccctc
     3601 actgcgagcc agcgacgcgg agcggcactg ttcttcacga aggctggaga gggtggcgcc
     3661 ggttgcttct cttgtcacag cgggccgatg ctcaacaagc agccaaacga cccggacgtc
     3721 accgggatcg ggcacttcgt ggaggagaac ttcttcaata tcgggatcgg cgaccatccc
     3781 ctgcaggcgt tgaatgcgat gcggcgcggc cggctcaggt tcgatgcgga tgggaagccg
     3841 ctgtctcacg gcgaagacac gggtcgccaa gagatcacgc agaacccgga ccacgcctac
     3901 aagtttcgca gcttgacgct gcggcagctg aaggacgcga ggaccttctt ccacaacggc
     3961 tcgttcacta atctgcgaga tgtagtgagc tacttcaacc gtggcatccc gcaggatcgc
     4021 gagtttgcgg gcgcggccag gaccctggag ccgcgcttca ctaacccgcg cggagcgaga
     4081 gcgccgcgtg gcctcggcct gagtgacgat gacgtcgacg cgctcaccga tttcatcgaa
     4141 aacgggctct acgatccgac cttcgttcag tcgttccagc cgaacgaagc cgacctgaac
     4201 tattcgaaga accaccccga gctcgctgcg ctcggagcaa aggacggaca gctcttgagc
     4261 ggccttgcca tggacaacga tgacgcgctc tcgcgtcggg accaaggcct cgagtttctc
     4321 gacgtgagcc agagcgtgcg caacgagcgg at
//
LOCUS       DASTKS010000478         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_76796,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000478 DASTKS010000000
VERSION     DASTKS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76796"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFQ35_09950"
     CDS             complement(<1..302)
                     /locus_tag="VFQ35_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET9931001.1"
                     /translation="MASNYWLAKSEPFKFAWEQLVKDKRTTWDGVRNFEARNNLRAMK
                     KGDLVLFYHSNEGKEIVGIAKVAKEAYPDPTADEGDWSVVDLVPHKPLKQPVTLAQ"
     gene            388..1800
                     /locus_tag="VFQ35_09955"
     CDS             388..1800
                     /locus_tag="VFQ35_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9931002.1"
                     /translation="MTAATDDRLIVANSTPIRRAPTGQFTELSGWGNNRRVPCRLLEP
                     ETPTQVAAWIDRAGTVARGLGRSYGDAATNANRQVLGLARLDAYIAFDEATGTLTCDA
                     GVSLAQIIRHFTPRGWFPMITPGTKYVTIGGCIANDVHGKAHHVQGSFSNCVDSMRVL
                     LASGEIVVASREQNPDLFWATFGGMGLLGIVLTATIRLRRIETTYFRQRSFPVKNLAE
                     MLAVLAEQDQAFPYSVATIDVFAAGPRLGRGVVTVGEHASLAELPSEARKDPLRGLAN
                     ARLPVPFELPDFVLNPLSMRAVNAVIQQIQARAKPFGHFEGFFYPLDGILNWNRGYGK
                     RGFTQYQFAIPFDDGLRRLEQLLTAIFSAGELPFLNVLKRFGKASEGHLSFPTEGYTL
                     AIDFPIRKNTPELLKRLDAMVLDAGGRIYLGKDSFLDAASFRAMYPRLESWLDIKAKY
                     DPNHVFTSDLGRRVGLVPGG"
     gene            complement(1835..>2759)
                     /locus_tag="VFQ35_09960"
     CDS             complement(1835..>2759)
                     /locus_tag="VFQ35_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HET9931003.1"
                     /translation="LDQALLRPGRFDRRVIIDRPELGARRAILQVHTRGKPLAPDVDL
                     GQIAGNTPGFSGADIANLVNEAALNATRRGADAIAKSDFTAAYDKIVLGDLRETKLDP
                     QEKRRVAVHEAGHALVAHFSSNAEPLERVTIIPRGMALGVTQYVPGQDRHLFTEPQLR
                     DNLGMMLGGYAAERLVLGSISSGAENDLKRATELAFKMVAHWGMSERVGPVYHEHRTE
                     HPFLGATLATESGTSDATVHIIEDETRRILGGALNYAEGLLAEKRTELDLLVSALLDT
                     ETVEKDEIVRLLGPGSAGSAREGTATAAFAS"
BASE COUNT          443 a          913 c          899 g          504 t
ORIGIN      
        1 atttgggcga gagtgaccgg ctgcttcaac ggcttgtgag gcaccaaatc caccaccgac
       61 caatcgcctt cgtcggcggt tgggtcgggg tacgcctctt tggcgacttt ggcgatcccg
      121 acgatctcct tgccctcgtt cgagtggtag aaaaggacga ggtcgccctt cttcatcgcg
      181 cgcaggttgt tgcgcgcctc gaagttgcga acaccgtccc acgtggtccg cttgtctttg
      241 acgagctgtt cccaggcaaa tttgaagggt tcgctcttgg cgagccagta gttcgacgcc
      301 atttcgccgg cagtttaccc aatccggggc tcgggtaaac gccgaggcgc tggtcaacga
      361 ggtccgcaga gctagaattg ggccgctgtg accgccgcca ccgacgaccg cctgattgtc
      421 gcgaacagca ctcccattcg ccgcgcgccg acggggcaat tcaccgagct ctcggggtgg
      481 ggaaacaacc gacgcgtccc ttgccggctg ctcgaacccg aaacgccgac tcaagtcgcc
      541 gcctggatcg atcgcgccgg cactgtcgct cgcgggcttg gtcgcagcta cggcgacgcc
      601 gctaccaacg cgaaccgcca agtgctcggc ctcgcgcgac tcgatgcgta catcgcgttc
      661 gacgaggcga ctggcaccct cacctgcgac gcgggagtga gcctcgcgca aatcattcgg
      721 cacttcaccc cccgcggctg gttccccatg atcacgccgg gcacgaagta cgtaacgatt
      781 ggcggctgca tcgccaacga cgtgcacggt aaggcgcacc acgtccaggg cagcttcagc
      841 aattgcgtcg actcgatgcg cgtgttgctc gcgagcggtg agatcgtggt cgcgagtcgc
      901 gaacagaacc cggatttgtt ctgggcgacc ttcgggggca tgggcctgct cggcatcgtg
      961 ctcaccgcga cgattcgcct gcgaaggatc gagacgacct actttcgcca gcgctccttc
     1021 ccggtgaaga acttggccga gatgctggcg gtgctcgccg agcaagacca agcgttcccg
     1081 tattcggtcg cgacgatcga cgtgttcgcg gcggggccgc gcctcggtcg gggggtggtc
     1141 acggtgggcg agcacgcgag cctcgcggaa ctgccgagtg aagcgcgaaa agatccgctg
     1201 cgggggctcg cgaacgcgcg cttgccagtc cctttcgagc tacccgattt cgtattgaat
     1261 ccgctcagta tgcgcgcggt gaacgcggtc attcagcaga tccaagcgcg cgcgaagcca
     1321 ttcggtcact tcgaagggtt cttctacccc ctcgacggaa tcctgaattg gaaccgcggc
     1381 tacggtaagc gaggcttcac tcagtaccag ttcgcgatcc cgttcgacga cgggctgcgc
     1441 cggctcgagc agctgctcac ggcgatcttc tctgccggcg agctgccctt cctcaacgtg
     1501 ctcaagcgct tcggcaaggc gagcgagggg cacctctcgt tcccgaccga gggctacaca
     1561 ctcgccatcg acttcccaat ccgcaagaac acgcccgagc tcctgaagcg cctcgacgcg
     1621 atggtgctcg acgcgggcgg tcgcatctac ctcggcaagg attctttcct cgacgcggcg
     1681 agcttccgcg ccatgtaccc acggctcgaa agctggctcg acatcaaagc gaagtacgat
     1741 ccgaaccacg ttttcacgtc ggatctcggg cgtcgcgtcg gcttggtgcc cggcggctga
     1801 ggcctcgcct ggtcctcggc cttcgccgag tcgcctacga cgcgaacgcg gccgttgcag
     1861 tgccttcgcg cgctgagccg gccgagccgg gcccgagcag gcgaacgatc tcgtctttct
     1921 ctaccgtctc cgtgtcgagc agcgccgaga ccaacaaatc gagctccgtg cgcttctcgg
     1981 cgagcaggcc ttcggcgtag ttcagcgcgc cgcccaagat acgtcgcgtt tcatcctcga
     2041 tgatgtgcac ggtggcgtcg ctcgttccgc tctcggtcgc gagcgtggcg cccaagaagg
     2101 ggtgttcggt gcggtgctcg tggtacacgg gtccgacgcg ctcgctcatg ccccagtgcg
     2161 cgaccatctt gaaggcgagc tccgtcgcgc gcttgaggtc gttctccgcg cccgaagaaa
     2221 tgctgccgag cacgaggcgc tccgccgcgt acccgccgag catcatgccc aagttgtcgc
     2281 gcagctgcgg ctcggtgaag aggtggcggt cctgtccagg cacgtactgc gtcacgccca
     2341 gggccatccc gcgaggaatg atggtgacgc gctcgagggg ctcggcgttg gaagagaagt
     2401 gcgcgaccag cgcgtggccg gcttcgtgaa cggcgacgcg acgcttttct tgcgggtcga
     2461 gcttggtctc gcgcagatcg ccgagcacga tcttgtcgta cgcggcggtg aagtcgctct
     2521 tggcgatggc gtcggcgccg cgccgcgtcg cgttcaacgc cgcctcgttc accaggtttg
     2581 cgatgtcggc gccagagaag cccggcgtgt tccccgcgat ttgcccgaga tcgacgtcgg
     2641 gggcgagggg cttgccgcgc gtgtggacct gcaagatcgc gcgccgggcg ccgagctcgg
     2701 gccggtcgat gatgacgcgc cggtcgaagc gcccgggccg gagcagcgct tgatcgagc
//
LOCUS       DASTKS010000479         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_298777, whole genome shotgun sequence.
ACCESSION   DASTKS010000479 DASTKS010000000
VERSION     DASTKS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298777"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VFQ35_09965"
     CDS             complement(<1..1045)
                     /locus_tag="VFQ35_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9931004.1"
                     /translation="MTALPDTTAKPRSERARPLRVLMILESDFMKQGGGGAESQVRTL
                     AAHLVKLKQGVTVVVPMLARGSQVKTERCQKIPVARIQYPYWRVIGGGIMCARFAAFL
                     IANRNRYDAWHVHIAHHLGAVACLLGPLLNKPVIVKISGFWELERGIVAQKRSLLTKL
                     ARACFQRATVLQAISTRIAHDLVEQGFPEDRVVVLPNAVDTRRFHVESAPPEGPFTAV
                     FVGRLVPEKDLTVLLRGWARAFRGQTDTRLMLVGRGPEEQSLRALAGELGIAEQVAFL
                     GHCDSVQDLLARSHIGLLPSRVEGLSNTLLEYMASGLPTIATRVSGSEDFVDHERSGW
                     LFEVGDEQALADHL"
     gene            1618..3807
                     /locus_tag="VFQ35_09970"
     CDS             1618..3807
                     /locus_tag="VFQ35_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931005.1"
                     /translation="MAALIFLPFVAYAYLSPAVYRTTAVVRIKPINQSDVRMPPPLET
                     ARKLRDATLDDELIERLARDKSPASITEAQELRTEFELETVDGISFNVSFRGSDPVHT
                     QAVCNHLARRVVQSASRALAAHAEDEGARSDRIDELMALLSAAPDAAAPTPSAVASAA
                     QKEELARSGLRAERARLSEQLESAVPGSDNPYAAGETSPEVLKRRIAEIDRMLAARPK
                     AAPQNSPAEVDAKFRELVAGLATTDDRTAAAAPTVSAELTAEAPLPTTPVEPKRRLVL
                     LIGSLTALGAAGLVVIGRASARPRGRRSSRRAASQTHRITVATAQPPGERNVVPSVAQ
                     VFSAQEPEGSTTAPLDTHMPQEPKPAIVPHGVIDVGTAAAQAEPRGGSEIPSPPPPPP
                     PTQVEPRPQPARQPYGPRRVTQVLGSQAHLLTSGDAWSYAQNAGQPGLQSPDAPAPDT
                     VTAGSNYSEPAPAPAVQGSQPPAAAHGSGYPSGGYAGGGYTSSAPPRHAASPTPPREG
                     DYSSSRPPPPTARLIPIDATARFRPSQNLVPNARRGLRDSLLPLLRQPGFVVGIVGSD
                     EVGPAKAHVAAELALALASSAQFRLLLVECDFAAPSLTQLLRVDVPLFAGFSLQLQTR
                     SREGAEDVGWSVLRCTGSLDLLGESGLRAPDALRGPTFEQCIGSICRNYDIVILNGPL
                     LSQSEDCQAIGAVTDALVAVSRRDPQSVVQEVGQRVSSRVPIRVEPA"
     gene            3834..>4210
                     /locus_tag="VFQ35_09975"
     CDS             3834..>4210
                     /locus_tag="VFQ35_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931006.1"
                     /translation="MIRNNLRAALQAVRSRNASSRSQRGDVTVRVVDTSSEFRELATQ
                     WEELQRDAAITSVFETHDWQYLWWSTYGRGQPLRLLIATSQGRLVGVLPLYVQVQNPL
                     RYPVRVLRFVGVGGDTAPDDLGP"
BASE COUNT          708 a         1463 c         1346 g          693 t
ORIGIN      
        1 acaagtggtc cgccaacgcc tgctcgtctc ccacttcgaa caaccaaccg gagcgctcgt
       61 gatccacgaa gtcctcgctg ccgctcacgc gggtcgcgat ggttggcagg ccgctcgcca
      121 tgtactcgag cagcgtgttc gagagacctt cgacccggga gggcaaaagc ccgatgtgag
      181 accgcgcgag caggtcctgc acgctgtcac agtgcccgag gaaggcgact tgctcggcga
      241 tgccgagctc gccggcgagc gcgcgcaacg actgctcttc cggacctcgc ccgaccagca
      301 tcaagcgcgt atcggtctgc cctcgaaaag cccgcgccca gccacgcaac aagacggtca
      361 ggtctttttc cggaacgaga cgccccacga agacggcggt gaaaggtcct tcggggggcg
      421 ccgactcgac gtggaagcga cgcgtgtcga ccgcgttcgg gagcaccacc acccgatcct
      481 cggggaagcc ttgctcgacc aggtcgtgcg cgatgcgcgt gctgatcgcc tgcaagaccg
      541 tcgcacgctg aaagcaagct cgggcgagct tcgtgagcag gctgcgcttc tgcgcgacga
      601 tgccgcgctc gagctcccaa aacccagaga ttttcacgat caccggcttg ttcagcaatg
      661 ggccgagcaa acacgccacc gcgcccaggt gatgcgcgat gtgcacgtgc caagcatcgt
      721 aacgattgcg gttggcgatc aagaacgccg caaaccgcgc gcacatgatg ccgccgccga
      781 tcacgcgcca atacggatac tggatgcgcg cgaccgggat cttctgacac cgctccgtct
      841 tcacctggga cccgcgcgcg agcatcggca cgaccacggt cactccctgc ttcagcttca
      901 ccaagtgggc ggcgagggtg cggacctgcg attcggctcc gccaccaccc tgcttcatga
      961 aatcactctc gaggatcatc aacacacgga gtggccgtgc gcgctcggag cgaggctttg
     1021 ccgtcgtgtc cggaagcgcg gtcacgccgt gaatgtctgc gtgcttcgag tcgcgcgctt
     1081 tcgtgtgcct tgcttcgatt ggtgcggata acctcgaaga ggtgatcgag ggggtcatat
     1141 cgatggtcat gaagctctcg ctgaagtgag cgttgattcg ggccaccttt gcacaaaccg
     1201 tacaacgcac gtccgcgtga cgcactcccc gcctgcgtga agtcccacgt ggattccgtt
     1261 aatgtaactt gcagctttcg ttgcagaagg tagagcgcat gttcaactcc cagaggtcat
     1321 tcgtgtcgct agcagcctga atgccacgcc tcatcgacgc gcacgcgtcg cgctcgcgac
     1381 ggcactcggt gagtcgcacc aaaacgagaa agcgtgcgct gcctgctcgg ccggatcgcc
     1441 aggggagggc ctgttttggc ctgcggtcga aggcggtacc atcgcccccc tggtcgcgtt
     1501 aagctgcggg cccgtggcac ttttgcccag cattccggcc cttcctttct cccgcctagc
     1561 ggcccgagct ctatgagagt gcaagagacc gagcgtcccg tcctgctagg tgcttgcgtg
     1621 gccgcgctga tcttcctgcc gttcgtggcg tacgcctatc tgtcgcccgc cgtgtaccgc
     1681 accacggccg tcgtgcgaat caagccgatc aaccagagcg acgtgcgaat gccgcctccg
     1741 ctggaaacgg cgcgaaagct gcgcgacgcc acgctcgacg acgagctcat cgagcgcctc
     1801 gctcgtgaca agtcgcccgc ctccatcacc gaagcacagg agctacggac ggagttcgaa
     1861 ctcgagacgg tggatgggat atcgttcaac gtctcgtttc gcgggtctga cccagtgcac
     1921 acgcaagcag tgtgcaacca cctcgcccgg cgcgtcgtgc agtcggcgag ccgcgccctc
     1981 gcggctcacg cggaagacga aggcgcgcgc agcgaccgca tcgacgagtt gatggccctg
     2041 ctcagcgccg ctccggacgc ggctgctccg acgccttctg ccgtcgcttc tgccgcgcag
     2101 aaagaagagc tcgcgcgctc gggtctgcgc gccgagcgcg cgcggctgag tgagcagctg
     2161 gagagcgccg tccctggctc ggacaacccg tatgcggcag gggagacctc tcccgaggtg
     2221 ctgaagcggc gaatcgcgga gatcgatcga atgctcgcgg cgcgcccgaa ggcggcaccg
     2281 cagaactctc cggccgaggt cgacgccaag ttccgcgagc tcgtcgccgg tctcgccacg
     2341 accgacgacc gaacggccgc agcagctccg accgtcagcg cggagcttac ggccgaggcg
     2401 ccgttaccga cgacgccggt cgagcccaag cgtcggctcg tcttgctgat tggctcgctc
     2461 acggcgctcg gcgcggccgg gctagtcgta attggccgtg cttcggcgcg tccgcgcggc
     2521 cgccgctcgt cacgccgcgc cgcgagccag actcaccgca tcacggtcgc caccgctcag
     2581 ccaccgggcg agcgcaacgt cgtaccatcc gtggcgcagg tattctcggc gcaggagccc
     2641 gaaggctcga cgacggcgcc gctcgacact cacatgcccc aagaaccgaa gcccgcgatc
     2701 gtgccgcacg gcgtgatcga cgtcgggacc gcagccgcgc aagcagagcc tcgcggcgga
     2761 agcgaaatcc cgtcgccgcc tcctcccccg ccacccactc aggtcgaacc gcgcccgcag
     2821 ccggcgcgac aaccttacgg tccgcgccgc gtcacgcagg ttctcggttc tcaggctcac
     2881 ctcctcacgt ccggcgacgc ctggagctac gcgcaaaatg cagggcagcc cgggctgcag
     2941 tcgcccgacg cgccggctcc ggatacggtc actgccggca gcaattattc ggagccagcg
     3001 ccggccccag cggtgcaggg ctcgcagccg cccgcggcgg ctcatggcag tgggtatccg
     3061 agcggcggtt atgcgggtgg cgggtacacg agctcggcgc cacctcgtca cgcggcgtcg
     3121 ccgactcccc cacgagaagg cgattactcg agctctcgcc caccgccacc gaccgcgcgt
     3181 ctcattccga tcgacgcgac cgctcgtttc cgcccgtcgc aaaacctagt gccgaacgct
     3241 cgacgaggct tgcgcgactc gttgctaccg ctgttgcgcc agccgggctt cgtcgtgggc
     3301 atcgtgggga gcgacgaagt gggtccggcc aaagcgcatg tcgcggccga gctcgcgctc
     3361 gcgctcgcaa gctccgctca gtttcgcctg ctgctcgtcg aatgcgactt tgcggcaccg
     3421 agcttgacgc agctgctgcg cgtcgacgtc ccgctcttcg caggcttctc gttgcagctt
     3481 cagacccgct cacgtgaagg cgccgaggac gttggctggt cggtcctgcg ctgcaccggg
     3541 tcgctcgact tgttgggtga gagcgggctc cgggctcccg acgcgctgcg cgggccaacg
     3601 ttcgagcaat gcatcggctc catctgtcgg aattacgaca tcgtgatctt gaacggcccg
     3661 ctgttgtcgc agtccgagga ctgccaagcg atcggcgcgg ttactgatgc tttggttgcg
     3721 gtgagccggc gcgacccgca gagcgtcgtt caagaagtgg gacagcgagt gtcatcgaga
     3781 gtacccatcc gcgtcgagcc cgcgtgaccc ggccgccttg gaagatacgt aaaatgatac
     3841 gcaacaatct cagagccgct ctgcaggcgg tccgctcgcg caacgcgagc tcgaggtcgc
     3901 aacgcggcga cgtgaccgtg cgtgtggtcg ataccagctc ggagtttcgc gagctcgcca
     3961 cgcagtggga agagctccag cgcgacgccg cgatcaccag cgtgttcgag acgcacgact
     4021 ggcaatactt gtggtggtcg acctacgggc gcggacagcc tctccgcctc ctcatcgcga
     4081 cgagccaggg ccgcctcgtc ggggtgctgc cgctctacgt gcaggtgcag aacccgctgc
     4141 gctatccggt gcgggtgctc cggttcgtgg gcgtgggcgg cgacactgca cccgacgacc
     4201 tcggcccaat
//
LOCUS       DASTKS010000480         6545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1862139, whole genome shotgun sequence.
ACCESSION   DASTKS010000480 DASTKS010000000
VERSION     DASTKS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862139"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3737)
                     /locus_tag="VFQ35_09980"
     CDS             complement(<1..3737)
                     /locus_tag="VFQ35_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931007.1"
                     /translation="MRLLGLIRKLFASFALACLFLAASIVAFGLHLDLPRGRRVVSSL
                     VGRTLASELRGRFAVGGIERLRPGGAVLRDFRVWDERGREVLVVSKVTLRADLLWILR
                     ELVVSPARARLVVEHVEIEQADARIYDDAANGVPSLALAFAPRPRTTKSPSTTPSALS
                     LDVRALSLSHGYARGRVGALPTLELEVNQLRGALRFQNEVLDLELGRFGAVVRGVGGA
                     DARGVGSLRLHEPGWVWTSFDGYFGDVQVGSVLRVEGDEVTATLTLPRAEPRAMRGLL
                     PAYPVKKDAAVKAHAVGRVPDLALDAQVEIGETRATGSGTLKLGPRPILDLDITANAV
                     DAAALVEGLPPSQIDASAHLVVQSTPGQPSVSVRAVTLPGSVDRIAVPAAEVDAVFEG
                     SILRGKAQLHEPGLPIRGEFALSPGGIFDFHAESPSVVLSRAPRLRAIPGNGSAALRV
                     DAHVESGRLRASASGDVRDYQNDDLSIARAKFGARASGPVNDWQRLNVDAHVEGEDVG
                     VAGLRFARANATAQGVLAKPRVTLALDDARGGKLELSALVTALQKEKRVDDLSLSYKR
                     EQSELSGKLGSLKVGEGELALEGLSLRGLGGELTGSLEWSSRRASVDARGRQLRIGDI
                     ARLVGLPSHYVSGTLDFDAEVMLARDIERGSLNAKLTEGSLGPLPDVTSALNASLESG
                     RVQGDVSADIRNVATSSARFDIQLPGGVREMTSWRDAIGEISADVGQINLGLLSQVLA
                     PGKTPPVQGRLAVTLRATRRVPYALPSLALAGATTGLVVQPALFHPRAPGLDGVEAQF
                     GVNVSGESGASDASLRLVDARGALASASLSTTLDLAELVKDPRATLVGLPKLPVLGKV
                     LIDERQLSEFPRGLLPPIGDGKLRLEATLTGSFDAPVVAGRLKLGGFELAEPASTRPL
                     DLCASVAWDQSTRRLGSSGELFLSSAKSPTCGGSRVALYTVLAGLAHAAPGAPERLEG
                     SAVLSLERLPVDAIPGLGQSGLRGRVSGKASFTQSGPVPAFSANLTLREARVFDIPVG
                     DGKLELRSNEQALGVTLDLQQTQGSLQGTALVALDYSGLFPTLKSGEAIGVRVAARRA
                     DAVMLSPFVKDVLTDLTGTLDADLTARITRQDDENQPRAGRVQGSLALHGGSFELSGL
                     GLKLQDVDFSALAEEDAGGTRISVPLLTGHSNQGRRAVAVRNARVWLQGFTLARAEGS
                     VDASELPLTLPGIPQATATTRQSIAFEVRRTDREMQANITVP"
     gene            complement(3843..5867)
                     /locus_tag="VFQ35_09985"
     CDS             complement(3843..5867)
                     /locus_tag="VFQ35_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET9931008.1"
                     /translation="MPPEPLVDSVRVDGTRAVDAEKLRAGLATAETPLLFGIFPRVLE
                     YQVFDANVLARDLERVERYLRARGYYEAKVVAARVLRTGPHSVRVEIEVHEGQPVRVA
                     RIDPGLSSLPFDVMLEVNRAREMADGDIFDEARFEDDRQRIERVLLDAGYAFAKVEAK
                     ASVDIAAHEAHLVYRIEAGKKAHYGTVTIAGLSEIPEGPVRDNLELHEGDVYNESELE
                     DAERALVNLGVFASVEVRPDRTHPETGAVPILVRIRESALRSLRLGGGARFDVLRLSA
                     SLTAAWEHRNFLGGMRRFNVSGRPGVTLFPTRIGRIEPWTSYLPESYVTTELRQPSFI
                     EGRTTGKLNLQLSAYPLLYPYEGNAPEKEPIIGYFEVKASTGVQRNFFGHHLLIAPSY
                     NWQANFPFYYQNAQPTKPDSVRVSFPELVTTLSFVDDPVDTHSGFTLSNSLQVAGHGL
                     GGTVSDVRVAPEIKGYLPVRKAVLAARLGFGFLFPSGYGDLAFRGGRDLDARSPAVIA
                     DQQKLLFRAFYSGGPNSNRGYTYRGVGPQGPIGFLLPTGLNCNAAAAEPLPSGCILPI
                     GGLTLWEASLEARIPLPVGVPLSGVLFVDASDLTSEVGVVRLNAPHLSPGVGLRYMTP
                     VGPIRLDVGYRVPGAQQPASSETSANYAPEPRELFGFFPGAVHLAIGQAY"
     gene            complement(6015..>6545)
                     /locus_tag="VFQ35_09990"
     CDS             complement(6015..>6545)
                     /locus_tag="VFQ35_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931009.1"
                     /translation="YTIWGAGYSLRSRVHRKEVAGKKLTLVGYINKTNLADAPKCAVH
                     KGGKADPENCKPPIPAFWICDAKDSPLQECIKVMGWASNFAQIYDAIAEFDKGKENAE
                     YNDTFWGVKIPNPLPAVGAKVSITGNYGVTFSQSSTGAEADPVMGLMSLQELKELEPA
                     PELATLPGVKRKPPKK"
BASE COUNT         1072 a         2192 c         2199 g         1082 t
ORIGIN      
        1 ggcaccgtga tgttggcttg catctcccgg tcggtgcgac gcacttcgaa tgcgatgctt
       61 tgtcgcgtgg tcgcggtcgc ttgggggatg ccgggcaaag tgagcggcag ttcgctcgcg
      121 tcgacgctgc cttcggcgcg cgcgagcgtg aagccctgca gccagactcg cgcgttccgc
      181 actgcgaccg cgcgccggcc ctgattggaa tgcccggtga ggagcggcac gctgatgcgc
      241 gttcctcccg cgtcctcttc ggcgagcgcg ctgaagtcga cgtcctgcag cttcaggccg
      301 agcccgctga gctcgaagct gccgccgtgc aacgcgagcg agccttgtac gcgacccgcg
      361 cgaggctggt tctcatcgtc ttggcgggtg atgcgagccg tgagatcggc gtcgagggtg
      421 ccggtcaagt cggtcagcac gtccttcacg aagggcgaca gcatgaccgc gtcggctcga
      481 cgcgcggcga cccgcacccc gattgcttca ccggacttga gggtgggaaa cagtcccgag
      541 tagtcgagcg cgacgagggc ggtgccttgc agcgagccct gggtttgctg aaggtcgagt
      601 gtcacaccga gcgcttgctc gttcgagcgg agctcgagct tgccgtcgcc gaccgggatg
      661 tcgaaaacgc gcgcttcacg cagcgttagg tttgccgaga aggcaggtac cggcccgctc
      721 tgagtgaacg acgcctttcc gctcacgcgg ccgcgcaatc cggactgccc gagccccgga
      781 atcgcatcga ccggcagtct ttcgagcgaa agcacggcgc taccctcgag ccgctccggg
      841 gctcccggcg ccgcgtgagc gagacctgcg agcaccgtgt acagggccac gcgtgagccc
      901 ccacaagtgg gcgatttggc gctgctcaga aagagctcgc cgctcgatcc gaggcggcgc
      961 gtcgactgat cccacgcgac gctcgcgcag agatcgagcg gtcgggtgct cgccggctcc
     1021 gcgagctcga atccgccgag cttcaagcga cccgccacga ctggcgcgtc gaagctgccg
     1081 gtgagcgtgg cttccagccg aagcttcccg tctccgatcg gcgggagcag gcctcgcggg
     1141 aactccgaca attggcgctc gtcgatgagg accttgccga gcacgggaag tttggggagc
     1201 ccgaccagcg tcgcccgagg atctttcacg agctcggcga gatcgagcgt cgtcgagagg
     1261 ctcgcgctcg cgagggctcc ccgcgcgtcg accagtcgga gcgaggcgtc gctcgcgccg
     1321 gactcgccgc tcacgttcac tccgaactgg gcctcgactc cgtcgagccc cggcgctcgg
     1381 gggtggaaga gcgctggttg cacgacgagg cccgtggtcg ctccggcgag ggccaggctc
     1441 ggcagcgcgt atggaacgcg tcgcgtggcg cggagggtca ccgcgagccg tccctgcact
     1501 ggcggagtct ttcccggtgc gagcacttgg ctcaatagtc cgaggttgat ctgtcctacg
     1561 tcggcgctga tctcgccgat tgcgtcgcgc caagacgtca tctcgcggac gccgcctggg
     1621 agctgaatgt cgaagcgcgc gctgctggtc gcgacgttgc ggatgtccgc gctgacgtcg
     1681 ccctgcaccc ggccggattc gagcgaagcg ttcagcgccg aagtgacgtc ggggagtgga
     1741 ccgagcgatc cttccgtgag cttggcgttc aggctgcctc gctcgatgtc gcgggcgagc
     1801 atgacctccg cgtcgaagtc gagggttcca gagacatagt gcgagggcag cccgactagt
     1861 cgagcgatat cgccgatgcg aagctgtcgg ccgcgtgcgt cgacgcttgc gcgccggctg
     1921 gaccattcga gcgaaccagt gagctcgccg ccgagccccc gcagcgagag cccttcgagc
     1981 gcgagctccc cttcgcccac cttgaggctg ccgagcttcc cgctgagctc gctctgctcg
     2041 cgcttgtacg aaagagatag gtcgtcgacg cgcttctcct tctgaagcgc cgtcaccagc
     2101 gcagagagct ccaatttgcc gccgcgcgcg tcgtcgagag cgagcgtcac gcgcggcttc
     2161 gcgagaactc cctgtgccgt cgcgttggcc cgtgcgaagc gaaggccggc cacgccgacg
     2221 tcctccccct cgacgtgagc gtcgacgttc agccgttgcc agtcgttcac gggaccgctc
     2281 gcgcgagccc cgaacttcgc tcgtgcgatc gacagatcgt cgttttggta atcgcgcacg
     2341 tcgccgctcg cgctcgctcg caaccttccc gattcgacgt gcgcgtcgac gcgcagcgcc
     2401 gcgctgccgt tgccgggaat cgcgcggagc cgaggtgcgc gcgagagcac gacgctcggg
     2461 ctctcggcgt gaaaatcgaa gattcctccc gggctcagcg cgaactcacc tcggatcggc
     2521 aagccgggct cgtggagctg cgccttgccg cgcaagatgg agccttcgaa cactgcgtcc
     2581 acctccgccg cgggaacagc gatgcgatcg acgctgcccg gaagcgtgac ggcgcggacg
     2641 ctgacgctcg gctgcccagg ggtgctttgg acgaccaggt gcgcgctcgc gtcgatttgc
     2701 gatgggggca gcccttcgac gagcgccgca gcgtcgaccg cgttcgcggt gatgtcgagg
     2761 tctagaatcg gtcgcggtcc gagcttcagt gtcccgctcc cggtcgcgcg cgtttccccg
     2821 atctcgacct gtgcgtcgag cgcgagatcc ggaacgcgtc cgacggcgtg ggctttgacg
     2881 gcagcgtctt tcttcaccgg gtatgccggt agaagcccgc gcatggcgcg cggctccgcg
     2941 cgggggagcg tcaacgtcgc cgttacctcg tcgccttcga cgcgcagcac gctgccgacc
     3001 tgtacgtcgc cgaagtagcc gtcgaacgac gtccaaaccc agcccggctc gtgtaggcgc
     3061 aggctcccga ccccgcgtgc gtcggcgcct cccaccccgc gtaccaccgc gccgaaacgg
     3121 ccgagctcga gatcgagcac ttcgttctgg aaacggagcg ctccgcgcag ttgatttacc
     3181 tcgagctcca acgtcggaag agcgccgacg cggcctcgcg cgtaaccgtg ggaaaggctc
     3241 agcgctcgca cgtcgagcga tagcgcgctc ggcgtcgtcg acgggctctt cgtcgttcgg
     3301 gggcgcggcg cgaaggcgag cgcgagcgac ggcacgccgt tcgcagcgtc gtcgtaaatt
     3361 cgagcgtccg cttgctcgat ctcgacgtgc tcgaccacga ggcgcgctcg tgcgggagag
     3421 actacgagct cgcgcaggat ccaaaggaga tcggcccgca gcgtcacctt cgacaccacc
     3481 agcacttctc gcccgcgctc gtcccaaacc cggaagtcgc gcagcacggc gcccccgggt
     3541 cgaagccgtt cgatccctcc gaccgcgaag cgcccgcgca actccgacgc gagcgtgcgc
     3601 ccgaccaggc tcgaaacgac gcgtcgtcca cgcggcaagt cgaggtgcag tccgaatgcg
     3661 acgatgctag ccgccaagaa caagcaggcg agggcgaacg aagcgaaaag cttccgaatc
     3721 aggccaagca gccgcatccg ctacttagcc tagtctgcgt gcccgagagc gtcccgtcct
     3781 cgccgccgac gggggctcgt caggttgcca gtcgcgctct gggacacggt gggccgcttt
     3841 cttcaatacg cttgcccaat cgccaggtgc accgcgcctg gaaagaagcc gaagagctcg
     3901 cgcggctccg gcgcgtaatt cgccgaggtt tcgctcgacg ccggctgctg cgcgcccgga
     3961 acccgatagc cgacgtcgag ccgaatcggg ccgaccggtg tcatgtaacg tagcccgacg
     4021 ccgggcgaga ggtgcggcgc gttcaagcgc acgacgccga cctcgctcgt gagatcgctc
     4081 gcgtcgacga acaacacgcc ggagagcggc acaccgaccg gcagtgggat tcgcgcttcg
     4141 agcgacgctt cccacaacgt gaggccgccg atcggcaaaa tgcatccact cgggagcggc
     4201 tccgcggcgg ccgcgttgca gttcaagccg gtgggaagca aaaagccgat tgggccttgg
     4261 ggccccacgc cgcggtacgt gtagccacgg ttggaattgg gtccccccga gtagaaagcg
     4321 cgaaacagga gcttctgctg gtcggcgatc actgcggggc ttcgcgcgtc gagatcgcga
     4381 ccgccgcgga aggcgaggtc gccgtagccg ctcggaaaca aaaaaccaaa cccgagccgc
     4441 gcggcgagca cggcctttct cactggcaag tagcctttga tctcgggggc cacgcgtacg
     4501 tccgagaccg tgccgccaag gccatgcccg gccacttgca gcgagttcga gagcgtaaag
     4561 cccgagtgcg tatcgaccgg atcgtcgacg aacgagagcg tcgtgacgag ctcgggaaag
     4621 gagacgcgca ccgaatcggg cttcgtcggc tgtgcgtttt gataatagaa ggggaagttt
     4681 gcctgccagt tgtaggaagg cgcgatcaga aggtggtgac cgaagaagtt gcgctgaact
     4741 ccggtggagg ccttcacttc gaagtagccg atgatcggct ctttttccgg tgcgttgcct
     4801 tcgtacggat agagtagggg atacgcgctg agctgaaggt tcagcttgcc ggtcgtgcgc
     4861 ccctcgatga acgacggctg ccgaagctcg gtggtgacgt agctctcggg cagatagctg
     4921 gtccacggtt cgatgcggcc aatacgcgtc ggaaagaggg ttacgccggg ccgcccgctc
     4981 acgttgaaac gccgcattcc accgaggaaa ttgcgatgct cccaggctgc ggtgaggctt
     5041 gccgacaagc gcagcacgtc gaagcgagcg ccgccgccga gccgcaggga acgcagcgcg
     5101 ctctcgcgaa tccgaaccag gatcggcacc gctcccgtct ccggatgcgt gcgatcgggc
     5161 cgcacctcga cgctcgcgaa cacgccgagg ttcacgagcg cgcgctccgc gtcctcgagc
     5221 tccgactcgt tgtagacgtc gccctcgtgt agctcgaggt tgtcgcgcac cggcccctcc
     5281 gggatctcac tgagcccggc gatggtgacg gtgccgtagt gcgccttctt tccggcctcg
     5341 atgcgataga cgaggtgcgc ctcgtgggcg gcgatgtcga cgctcgcttt cgcttcgacc
     5401 ttggcgaagg cgtaccccgc atcgagcagc acgcgctcga tacgctggcg gtcgtcttcg
     5461 aagcgcgcct cgtcgaagat gtccccatcg gccatctcac gcgctcggtt cacttcgagc
     5521 atcacgtcga acggcagcga cgagagccca ggatcgatgc gcgcgacgcg cacgggctga
     5581 ccttcgtgaa cctcgatctc gacgcgcacg gagtgcggtc ccgtgcggag cactcgcgct
     5641 gcgacgacct tcgcctcgta atatccgcga gcgcgcaggt agcgctccac gcgctcgaga
     5701 tcgcgagcga gcacgttggc gtcgaacacc tgatactcga gcacccgtgg gaagatgccg
     5761 aacaagagcg gcgtctcggc ggtcgcaagc ccggcgcgta gcttttccgc gtcgaccgcc
     5821 cgcgtgccat cgacgcgcac cgagtcgacc aatggctcgg ggggcaatgg gcgacaggcg
     5881 cccaacgccg ccaggcatag gcatgacgca aaacgccgag ctccgatgag agagcccggc
     5941 gttttgtcga ttcggcgcga agtcaaagcg aagcgcgcct cgtgtcccgc gcggggcggg
     6001 agcatatcag ctcgctactt cttcgggggc ttgcgcttga cgcccggcag tgtcgcgagc
     6061 tcgggggcgg gctcgagctc cttcaactct tggagcgaca tcaagcccat gaccggatcc
     6121 gcctcagcgc ccgtcgacga ctgcgagaac gtcacgccgt agttgccggt gatgctgacc
     6181 tttgcgccaa ccgcgggcaa cgggttcggg atcttaacgc cccagaacgt gtcgttgtac
     6241 tcggcgttct ccttgccttt gtcgaactcc gcgatcgcgt cgtaaatctg cgcgaagttc
     6301 gaagcccagc ccatcacctt gatgcactct tggagcggcg agtccttggc gtcgcagatc
     6361 cagaaagcgg ggatcggcgg cttgcagttc tccgggtcgg ctttgccgcc cttgtgaacg
     6421 gcacacttcg gcgcgtcggc gaggttcgtc ttgttgatgt agccaaccaa cgtcagcttc
     6481 ttgccggcga cctctttgcg atgcacgcgg ctgcgcagcg agtagccggc gccccaaatc
     6541 gtgta
//
LOCUS       DASTKS010000481         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1416046, whole genome shotgun sequence.
ACCESSION   DASTKS010000481 DASTKS010000000
VERSION     DASTKS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416046"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFQ35_09995"
     CDS             <1..624
                     /locus_tag="VFQ35_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9931010.1"
                     /translation="VLRSLTGGPELYEPDGATAIIGAKGAINLPGERFCDAEEARLSY
                     RSERLWLEDLDRGNGVFLRIRTPVELELGDEFVVGDQLLRIERNPVPDDGPDSHPTYF
                     YSSPKWPSSFRVIQVFEGGALGCCVIARGNTLQIGSAVGDLVFPDDPLVGEQHCLIEE
                     HAGTVVLTDLGSRTGVFVRIRGEQELAHGDELLVGRTRLVVDLRSVA"
     gene            631..2625
                     /locus_tag="VFQ35_10000"
     CDS             631..2625
                     /locus_tag="VFQ35_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9931011.1"
                     /translation="MKAPTFAYAAIALALVACAKPPSLPPEPRAQVGAVSVQAADRVR
                     DVDELIAGGHLRSAYAEVLSAAARSASGELDCRRARLALWLGAYSDAAESLVRLSHAK
                     EARCDVAALGAEHRALSTSQKDEAGAQKLAERAARSSRERQYAAAKREYLRAWELAHP
                     NGAWLIAAGGEAALAGTTNEARALFDRGMSELERETGDHFSVALPTNVERLAAVEWSP
                     DGSLLALATPRSLVLVDSATLVERFVLPLGCNDLAWTAATGLICGHADGSVLRIDPRR
                     ETAVPLGKLPGPVRRVSANAGIVAAAGGGHAVFIDAQGSWSAADSGASAVAVSPDAKF
                     GVTGFSDGSLRRVSLVTHDVERLRAPMADASPVDRVRISPDSNWIGASFGGDRAVVFE
                     LGRARRSRSLPFIGDFRFAPDSKALLTSSATFALEEVTSRRSLTEQLFSSSAFAISPD
                     AESMFEQSAVAIGIRSRSAEHTVAERVTLELEGGWWFSLTELSEIAAHDGRFPASAAR
                     APTPSIPDCNEGPGVRAPSSGELLCASGELRLTRPGETKPRWSVRLPVEPVGYLEFTA
                     DAQRVLVGGPRMRAAIVNVGDGSTAAELRLYALDFDRGDFSSTGSVIDRDGNVEFWGE
                     QATELANQARCAVGTYRFDFAVCRDERSRRGTLAHVLGRR"
     gene            complement(2633..>3950)
                     /locus_tag="VFQ35_10005"
     CDS             complement(2633..>3950)
                     /locus_tag="VFQ35_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931012.1"
                     /translation="IRVLLAAQLAPTNGQGQKVLGVSVSFTRKGKGDTLATSSAQPAK
                     LVFFELPGKGKTPDANTSERKFATLSGTLALSGDAPVFDAGGKAIVYEAFVRDPRKSA
                     SAPTKSSDFRLNLDLAAPHFENTNDLQSENRSLFLPWDFDGENEKLEIGAQLEIGSSV
                     EASHSDNPPLDVPLAHRGVLEDGVGPKKRTYFGVGMVYPSVNLTFEDLKVSGKIAMPG
                     KAITINIHQDLDAKASSIATVASNLKTIFSDAGISATVNTSRTTAQANSVGWKRRKFS
                     RGSAFAADNFSDLDQDGPGGREFSFFEYWVFFETSLDGTSTEAGESEGIIAVNAKKAT
                     KVLKFPIHLLGGSSGGSERAFTQTLNQVSASDKDNFIANLIAHEVGHSLGLGHGLEVT
                     SSGYSLTGGSTDRTRGIVTTEHVVAGQAVLKFLSPVHKSLIRRDYF"
BASE COUNT          592 a         1253 c         1360 g          745 t
ORIGIN      
        1 gtcttgcgga gccttaccgg agggcccgag ctctacgagc ccgacggcgc gaccgctatc
       61 atcggcgcca agggtgccat caaccttccc ggcgagcgct tctgcgacgc cgaagaggcg
      121 cggctcagct accgctccga gcggctttgg ctcgaggatc tggatcgcgg caacggcgtc
      181 ttcctgcgca ttcgcactcc ggtcgagctc gagctcggcg acgagttcgt ggtgggcgac
      241 cagctgcttc gcatcgagcg caaccccgtc cccgacgacg gtcccgattc tcatcccacc
      301 tatttctact cgtccccgaa gtggccttcg tcgttccgcg tgattcaagt cttcgaaggc
      361 ggggcgctcg ggtgctgcgt catcgcgcgc ggcaatactt tgcaaatcgg ctcggcagtt
      421 ggcgatttgg tgttccccga cgatccgctc gtcggcgagc aacattgctt gattgaagag
      481 cacgcgggca cggtggtgct cacggatctg ggctcgcgca cgggagtgtt cgtgcgcatc
      541 cgtggggagc aggagctggc gcacggcgac gagctcttgg ttggccgcac gcgcctggtc
      601 gtggatttac gaagcgtagc ttagggccag gtgaaagcgc cgaccttcgc gtatgcggcc
      661 atcgcgctcg cgctggttgc gtgcgcgaag ccgccgagcc tgccgccaga gccgcgcgcg
      721 caggtgggag cggtctccgt gcaagcggct gatcgcgttc gcgacgtgga cgagctcatc
      781 gcgggcggtc acttgcggag cgcttacgcc gaggtgctga gcgcggcggc taggagcgcg
      841 agcggcgaac tcgattgtcg gcgcgcgcgc ttggcgctct ggctgggagc ctactcggac
      901 gcggcagaga gcttggtcag gctctcccac gccaaagaag ctcgctgtga cgtggccgca
      961 ctaggcgcgg aacaccgcgc gctttcgacc tcgcaaaagg acgaggccgg tgcgcagaag
     1021 cttgcagagc gagccgcgcg ctcgagtcgc gagcgccaat acgccgcggc caagcgcgag
     1081 taccttcggg cctgggagct cgcgcacccg aacggcgcgt ggttgatcgc ggccggtggc
     1141 gaagcggcgc tcgcgggaac gacgaacgaa gcacgggcgc tcttcgatcg cgggatgagc
     1201 gagctcgaac gcgagacggg ggatcacttc agcgtcgcgc tgccgacgaa cgtcgagcgc
     1261 ctcgcggccg tcgaatggtc acccgacggg tcgctgctcg cgctggccac tccgcgcagc
     1321 ttggtcctcg tcgacagcgc cacgttggtg gagcgcttcg tccttccatt gggctgcaac
     1381 gatttggctt ggaccgcagc gaccgggctc atctgcggtc acgccgacgg gagcgtcttg
     1441 cgcatcgatc cgagacgtga aacggcagtc ccgctcggca agctaccggg gccggtgcgc
     1501 cgcgtcagcg ccaacgccgg catcgtcgca gccgcgggcg gcgggcacgc cgtcttcatc
     1561 gacgcccaag gcagctggtc agcggcggac tctggcgcga gcgcggtcgc ggtaagccca
     1621 gacgcaaagt tcggcgtcac cggcttttcc gatggttcgc tgcgccgagt gtccctcgtc
     1681 acgcacgatg tcgagcgttt gcgcgcgccc atggccgacg cttcgccggt cgatcgcgtt
     1741 cgcatttcac ccgactcgaa ctggatcggg gcgtcgtttg ggggcgatcg tgcggtcgtt
     1801 ttcgagctcg gtcgagcgcg ccgcagccga agcctgccct tcatcggaga ttttcgcttt
     1861 gcgccggact cgaaggcgct cctcacgagc tcggcgacgt tcgcgctcga ggaggttacg
     1921 tcgcgaaggt cgctgaccga gcagttgttt tcgagctcgg cgttcgcgat ttcccccgac
     1981 gcggagtcga tgttcgagca gtctgcggtt gctatcggca ttcgttcccg gagcgcggag
     2041 cacaccgtcg ccgagcgcgt cacgctggag ctcgagggcg gatggtggtt ctcgctgacc
     2101 gagctcagcg aaatcgcagc gcatgacggt cggttcccgg cctccgcggc gcgcgcgccg
     2161 actccctcga ttccggactg caacgaaggt cccggcgttc gcgctccgag tagcggcgag
     2221 ctactgtgcg cctcgggcga gctccgcttg acgcgaccgg gcgagaccaa gccgcgttgg
     2281 tccgttcgtc tcccggtcga gccggtcgga tatctcgagt tcacggccga cgcgcagcgt
     2341 gtgctcgtcg gtggcccgag aatgcgggcg gccatcgtga acgtcggcga cggcagtacc
     2401 gcggcggagc tgcgcctgta cgctttggac ttcgatcgcg gcgatttctc gagcaccggg
     2461 tccgtgatcg accgggacgg caacgtcgag ttttggggcg agcaagctac ggagctcgcg
     2521 aaccaggcgc gctgcgccgt aggtacctat cgcttcgact tcgccgtgtg ccgcgacgag
     2581 cgctcgcgcc gcgggacttt ggcgcacgtg ttggggcgcc gataggccta cttcagaagt
     2641 agtcgcggcg aatgagcgat ttgtggacgg ggctcaagaa cttgagcacg gcttgccccg
     2701 cgacgacgtg ctcggtcgtg acgatgccgc gggtgcgatc cgtcgagccg cccgtgaggc
     2761 tgtagcccga cgaagtcact tccaaaccgt gtcccaagcc gaggctgtgc ccgacctcgt
     2821 gcgcgatgag gttcgcgatg aagttgtcct tgtccgaggc gctcacctgg ttgagcgttt
     2881 gcgtgaaggc ccgctcgctc cctcccgagc tgcccccgag caggtgaatc gggaatttca
     2941 ggaccttggt ggctttcttc gcgttcacgg cgatgatccc ttcgctttcg ccggcttcgg
     3001 tcgaggtgcc gtcgagtgag gtctcgaaga acacccagta ctcgaagaac gaaaactcgc
     3061 gacctccggg cccgtcctgg tcgagatcac tgaagttgtc ggctgcgaac gcggagccac
     3121 ggctgaactt gcggcgcttc cagccgacgc tgttcgcctg cgccgtcgtg cgcgacgtgt
     3181 tgacggtggc gctgatgccg gcgtccgaga agatcgtctt gaggttgctc gcgacggtgg
     3241 cgatgctgga tgctttcgcg tcgaggtctt ggtgaatgtt gatggtgatt gctttgcccg
     3301 gcatcgctat cttgccggac actttcaagt cctcgaacgt gaggttcacc gacgggtaaa
     3361 ccataccgac gccgaagtaa gtgcgcttct tcgggcccac tccgtcttcg agcacgccgc
     3421 ggtgcgcgag cggcacgtcg agcggtggat tgtcggagtg cgaagcctcc acgctgctcc
     3481 cgatctcgag ctgggcgccg atttccagct tttcattttc gccgtcgaag tcccacggca
     3541 aaaacagcga gcggttctcg ctctgaaggt cgttcgtgtt ctcgaaatgg ggcgccgcga
     3601 gatcgagatt cagtctgaag tcggagctct tcgtaggcgc tgacgcgctc tttcgaggat
     3661 cgcgcacgaa agcttcgtag actatcgctt tgccgcccgc gtcgaagacg ggcgcatcac
     3721 cgctgagggc gagcgtgccg gagagggtcg cgaacttgcg ctcactcgtg ttcgcgtccg
     3781 gcgtcttgcc cttcccgggg agctcgaaga agacgagctt cgcgggctgg gctgacgagg
     3841 tcgcgagcgt gtctcccttg cctttgcggg tgaagctgac cgacaccccg agcacctttt
     3901 gcccttggcc gttggtcggg gccagttgcg cagcgagcag gactcgaatt
//
LOCUS       DASTKS010000482         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_745498, whole genome shotgun sequence.
ACCESSION   DASTKS010000482 DASTKS010000000
VERSION     DASTKS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745498"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFQ35_10010"
     CDS             <1..197
                     /locus_tag="VFQ35_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931013.1"
                     /translation="GCVQAFIDGTLVAQRDISKDGVSPADYGAASSYVGFGNVLNREN
                     TEPTVWDYFGAGTIPPSSSP"
     gene            complement(305..1138)
                     /locus_tag="VFQ35_10015"
     CDS             complement(305..1138)
                     /locus_tag="VFQ35_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931014.1"
                     /translation="MRKPLSSSLIVAAALHAGLAHGEDVKQYADCAHEPTETDVTAAK
                     GAFQAGNASFEEADYARAIVYWEDAYRRDCTAHGMLLNLARAYELSGNKAQAVVALRT
                     YLVRVPTADKDKIERRIEVLNKQLDAERAQAAAQQQQAAQPPVAPAPATTPIAPPPPV
                     VERVKPPLAALVVAGVGGVAAIVGGVLFVTGKSDLAEADDKCPDRNNCGDPQVRADGN
                     SARTRATVGTWLGVGGVAMVGGGLAWYFLSPRTKSTAERQGFTPVALPSYVGLSYRTA
                     F"
     gene            1366..>2577
                     /locus_tag="VFQ35_10020"
     CDS             1366..>2577
                     /locus_tag="VFQ35_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9931015.1"
                     /translation="MLPERKQILVVDDEPNLRRVLTAQLERDGYDVHTAEDGEQALST
                     LREHHVDLVITDLRMPKLDGMELLRRVVAMDDELPVVMLTAHGTVDNAVEALKTGAFD
                     YITKPFDQAEVRTIVRKALKTRDLSSVEASRAAQAQDGGARYGIIGQSAGILELYAVL
                     DRVADTPTTVLITGESGTGKELVARALHENSSRRDKPFIKVNCAAIPKDLMESELFGY
                     ERGAFTGAVGSKPGRFELASGGTLFLDEIGSIPVEMQVKLLRALQESEFERVGGIKTI
                     RVDVRLVAATNSDLKKEIGLGTFREDLYYRLNVVPIRLPALRERAQDILLLVRHFIEK
                     FNARLKKSVTGIDANALALLSAYPWPGNIRELENVIERAVLFCDKTEIGISDIPNEIR
                     GAELGGANTPSP"
BASE COUNT          460 a          840 c          843 g          434 t
ORIGIN      
        1 atggctgtgt gcaggcgttc atcgacggca cgttggttgc gcagcgcgac atctcgaaag
       61 acggcgtttc gcccgcagac tatggggcgg cttcgtcata cgtcggcttc ggcaacgtcc
      121 tcaatcgaga gaacaccgag cccacggtat gggactactt cggcgctgga acgattcccc
      181 cgagttccag cccctaaagg gctcgcgtaa aaaaggctga cgggtgccgc cgagccactt
      241 gctgcggcgc aagtgctcgt agcctcatcc attcgcgagc gctaagcgct gctgagaacc
      301 caggttagaa cgcggtcctg tagctcagcc cgacgtagct cggcagagca accggcgtaa
      361 acccctggcg ctcggccgtg ctcttcgttc gcgggctcaa gaagtaccag gcgagcccac
      421 ctccaaccat tgcgacgcca ccaaccccga gccaagtgcc gacggtcgcc cgcgtgcgcg
      481 ccgagttgcc gtccgcgcga acttgcgggt cgccgcagtt gttgcgatcc gggcacttgt
      541 cgtcagcctc cgccaaatca cttttgccgg tgacgaacag aacaccaccg acgatcgcag
      601 cgacaccgcc gacgccagct acgacgagcg cggccaaggg aggctttact ctttcgacga
      661 cgggaggcgg aggcgcgatc ggcgtggttg cgggcgccgg ggccaccgga ggttgcgccg
      721 cttgttgctg ctgcgccgcg gcctgcgccc gctcggcatc gagctgcttg ttcagcacct
      781 cgatgcggcg ctcgatcttg tccttgtcag cggttgggac gcgaaccagg taggtacgca
      841 acgcgacgac ggcctgagcc ttgttgccgc tcagctcgta ggctcgggcc agattcaaca
      901 gcattccgtg cgcggtgcag tcgcgccgat acgcgtcctc ccagtagacg atggcccgcg
      961 cgtagtcagc ctcttcgaag gaggcgtttc cagcctggaa agctcccttg gcggccgtga
     1021 cgtcggtctc ggtgggctcg tgggcgcagt cggcatactg cttcacgtcc tccccatgag
     1081 cgagccccgc gtggagcgcg gcggcgacga tcaaggacga tgaaagcggc tttcgcatgc
     1141 gcggcacagg ccctccagta agtgggtcag atcgtcgata ccataggcag aaggtctgcc
     1201 gagaggtcaa tcgctccccg ggcgccgcgg ctcgagccgg cgcggtcggc cctgtggccg
     1261 cccatcgcgg gaagggggtg cgcgcagccc gcccgctcat cttcaatctg ggctacaccc
     1321 gaggctcttc cccctgctcg gcgagcggga gaaatcaagc tgcccatgct gcccgaacgc
     1381 aagcaaattt tggtcgtcga cgatgagccg aacctgcggc gcgtcctcac ggcacagctc
     1441 gaacgcgatg gctacgacgt gcatacggcg gaggacgggg agcaggccct ctcgaccctg
     1501 cgcgaacatc acgtcgatct cgtcatcacc gatctgcgca tgccgaaact cgacggcatg
     1561 gagctcttgc ggcgcgtcgt cgccatggat gacgagctgc cggtcgtgat gctgaccgcg
     1621 cacggcaccg tcgacaatgc ggtggaagcg ctcaagacgg gcgcgttcga ctacatcacg
     1681 aagccgttcg atcaggccga ggtgcgcacc atcgtgcgca aggcgttgaa gacgcgcgat
     1741 ctttcttcgg tcgaagcctc gcgcgcggcg caggctcagg acgggggcgc ccgctacggt
     1801 atcatcggtc agagcgcggg cattctcgag ctctacgcgg tgctcgaccg cgtcgccgat
     1861 acgccgacca ccgtgctcat cacgggcgag agcggcacgg gcaaagagct cgtcgcgcgc
     1921 gcgctgcacg agaactcttc gcggcgcgac aagcccttca tcaaggtgaa ctgcgcggcg
     1981 atcccgaagg acttgatgga aagcgagctc ttcggttacg agcgcggcgc cttcaccggc
     2041 gcagtgggct cgaagccggg tcgcttcgag ctcgccagtg gcggcacgtt gttcctcgac
     2101 gagatcggct cgatcccggt cgagatgcag gtgaagcttc tgcgggcgct gcaggagagc
     2161 gagttcgagc gcgtgggcgg catcaagacc atccgggtcg acgtgcggct cgtggccgcg
     2221 accaatagcg atttgaaaaa agagatcggt ctcgggacct tccgcgagga tctctactat
     2281 cgcctcaacg tcgtgccgat tcggctgccc gcgctgcgcg agcgcgcgca agacattctg
     2341 ctgctcgtgc ggcacttcat cgagaagttc aacgcgcgcc tcaagaagag cgtgaccgga
     2401 atcgacgcca atgcgctcgc gctcttgtcg gcctatccgt ggccggggaa catccgcgag
     2461 ctcgagaacg tcatcgagcg cgcggtgctc ttctgcgaca aaaccgagat tggcatcagc
     2521 gatattccga acgaaatccg cggagcagag ctgggcggtg cgaacacccc gagcccg
//
LOCUS       DASTKS010000483         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1192805, whole genome shotgun sequence.
ACCESSION   DASTKS010000483 DASTKS010000000
VERSION     DASTKS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192805"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFQ35_10025"
     CDS             complement(<1..234)
                     /locus_tag="VFQ35_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931016.1"
                     /translation="MKAAALVVIWTLASELSSPATNAMTGAASEALGPDTELRLEASP
                     DPEGIRLADVGNASAVVRLTWDESEHRRARITCF"
     gene            complement(276..1805)
                     /locus_tag="VFQ35_10030"
     CDS             complement(276..1805)
                     /locus_tag="VFQ35_10030"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9931017.1"
                     /translation="MDERELRELSERAAHGGADKYHRKNAEQGKLFARERIGLLLDPN
                     SFVEDALLANSEAGDLPADGVVTGLGRIGGRKVALMANDSTVKAGSWGKRTVEKILRI
                     QETAERLACPLLYLVDSAGARITDQVEMFPGRRGAGRIFYNQVTLSGKIPQLCLLFGP
                     SAAGGAYIPAFCDVVVMVDGNASMYLGSPRMAEMVIGEKVTLEEMGGAKMHTQVSGCG
                     DVLVKTEAAAIEWARTYLSYMPENYAEKPPHAASKPPRADAPRIADIVIPDENKSFDM
                     KRVIDALVDEGSFFEVKERFAKELITGFARIDGRVVGVVANQPKHLGGVLFVDSADKA
                     ARFIWLCDAFGIPLLFLADVPGFMIGTKVERQGIIRAGAKMIAAVSEASVPKISVVVR
                     KAYGAGLYAMCGPAFEPDACLALPTASIAVMGAQAAVNAVYYNRIQEVEAGPARDALV
                     NELREQYRRDVDLHKLASELVVDEVLSFDDLREGLVRRFALYADKAPKRPDKKHLVSP
                     M"
     gene            complement(1862..>2404)
                     /locus_tag="VFQ35_10035"
     CDS             complement(1862..>2404)
                     /locus_tag="VFQ35_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9931018.1"
                     /translation="RHPGERVALAVNRHGDNRLFSADLEALPNEEALMKKRFLNAAAP
                     SFGQLLAVQGNTEPNLRALKGRVVVLEFWATWCVPCRLTAPRLTDWSDRHTAEGLSVL
                     GITTDPVPLAAEGARKASMSYSVFSDDSGDTVRGYKAFALPTLFVIDRRGVVRDIVVG
                     YSSERLRQVDGLLKRLLAER"
BASE COUNT          404 a          821 c          764 g          415 t
ORIGIN      
        1 gaagcaggtg atgcgcgcgc ggcgatgctc cgactcgtcc caggtcagcc gaaccacggc
       61 gctcgcgttg ccgacgtcgg cgaggcggat tccctccgga tcgggcgacg cttcgaggcg
      121 aagctcggtg tccggaccaa gcgcttcgct agcggcgccg gtcatcgcgt tcgtagccgg
      181 ggacgacagc tccgaagcga gggtccaaat cactacgaga gcggcggcct tcacgcgacg
      241 ctccgaggtt acaccgcctt cgaccagggt ggcagctaca tcggcgagac gaggtgcttc
      301 ttgtcgggcc gcttcggggc cttgtccgcg tagagcgcga agcggcgcac gagaccttcg
      361 cgcaaatcgt cgaacgagag gacctcgtcg acgaccaact cgctcgcgag cttgtgaaga
      421 tcgacgtcgc ggcgatactg ctctcggagc tcgttcacga gcgcgtcgcg cgcgggcccg
      481 gcttcgacct cctgaatgcg gttgtagtaa acggcgttca cggccgcttg tgcgcccatc
      541 accgcgatcg aagccgtcgg caacgcgagg catgcgtccg gctcgaaggc ggggccgcac
      601 atcgcataca ggccggctcc gtaggccttg cgcacgacga cgctgatctt gggaacgctc
      661 gcctcgctca cggctgcgat catctttgcg cccgcgcgga tgatgccctg gcgctcgacc
      721 ttggtgccga tcatgaaccc gggcacgtct gccaagaaca agagcgggat cccgaacgcg
      781 tcgcagagcc agatgaagcg cgcggccttg tcggcactat cgacgaacag gacgccaccg
      841 aggtgcttcg gttggttcgc gacgacaccc accacgcgcc cgtcgatacg agcgaagccg
      901 gtgatgagct ccttcgcgaa gcgctccttc acttcgaaga agctgccttc gtccacgagc
      961 gcgtcgatga cgcgcttcat gtcgaagctc ttgttctcgt cggggatcac gatgtcggca
     1021 atgcgcggag cgtcggcacg cggcggcttg ctcgcggcgt gcggcggctt ctccgcgtag
     1081 ttttccggca tgtacgagag gtacgtgcgc gcccactcga tcgctgccgc ctccgtcttc
     1141 acgagcacat cgccgcaacc ggaaacttgc gtgtgcatct tggcgccgcc catttcttcg
     1201 agcgttacct tctcgccgat caccatctcg gccatgcgcg gcgagcccag gtacatgctc
     1261 gcgttgccgt ccaccatcac caccacgtcg caaaacgcgg ggatgtaagc gccgcccgcc
     1321 gcggaaggac cgaacagcaa gcagagctgc ggaatcttcc cgctcagcgt gacctgattg
     1381 tagaagatgc gccccgcgcc gcggcgaccc gggaacattt cgacctgatc ggtgatgcga
     1441 gcgccggcag agtcgaccaa gtagaggagc ggacacgcga gccgctcggc cgtctcttga
     1501 atgcgcaata tcttctcgac cgtgcgcttg ccccacgagc cggccttcac cgtcgagtcg
     1561 ttcgccatca aggcaacctt gcgtccgccg atgcgtccga gcccggtgac gacgccgtcg
     1621 gcgggaaggt cgccggcctc cgagttcgcg agcagcgcgt cttcgacgaa gctgttcggg
     1681 tcgagcaata gcccgatgcg ctcgcgcgcg aagagcttgc cctgctcggc gttctttcga
     1741 tgatacttgt cggcgccgcc gtgcgccgcg cgctcgctca actctcgaag ttcgcgttcg
     1801 tccatcgcgt tctacggtaa cggctcgggc gtttcggtca atagccgagc gcggcgcgtg
     1861 ctcagcgctc cgcgagcaga cgcttcaata ggccgtcgac ttgtcgcaat cgctcgctcg
     1921 agtagccgac cacgatgtcg cgcacgacgc cgcgacggtc gatgacgaac aaggtcggca
     1981 atgcgaaggc cttgtagccg cgcacggtgt cgcccgagtc gtcgctgaac acgctgtacg
     2041 acatgctcgc cttacgcgcc ccctccgccg cgagcggcac cggatccgtc gtgatgccga
     2101 gcacactgag accttcggcc gtgtggcgat cgctccagtc ggtcaagcgc ggcgcggtga
     2161 gccggcacgg cacgcaccac gtcgcccaaa actccagaac gaccacgcgt cccttcagcg
     2221 ctcgcaggtt cggttcggtg ttcccctgca ccgcgagcag ctgaccgaac gacggcgccg
     2281 cggcgttgag aaagcgcttc ttcatcaacg cttcttcgtt ggggagcgcc tcgagatcgg
     2341 ccgagaacaa ccgattgtcg ccgtgacgat tcaccgcgag cgccacgcgc tctcccgggt
     2401 gtcg
//
LOCUS       DASTKS010000484         4766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_77954,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000484 DASTKS010000000
VERSION     DASTKS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77954"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..3222)
                     /locus_tag="VFQ35_10040"
     CDS             complement(121..3222)
                     /locus_tag="VFQ35_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9931019.1"
                     /translation="MRKSGDETLTTSDVVERRSYVTLLFSDVCDYTHIIEKLDPEDAD
                     ELRARIVELANRVVTKHGGQVNQFVGDGLLAVFGFPEPEENAVRRALGAALEFHRAAA
                     ELRFDAPGLRGFSARLHSGVHSGLVFLRRGNPLAGQYELSGDAVNTAARLCSAAGRDE
                     VIVSDSTLHGVEAFFKVQSVPALLLKGKSHPVAASRILGAAPEDEPARSRRGRASFVG
                     RERELTALYASLARAAHGQGSVICVLGDAGIGKSRLVSEFCRRARATLPQLRIHRGVC
                     ETYGDVAPLRPFLQILREVLPTPKDAPAEQSIWSVERALTQIDEGLRIHLTTLLGVLS
                     VRARERTSDANLDVIQAVTELMLALGKTTPLLLVLDDWQWADDASVQVMGRLVRAVGE
                     LPIQVVVMARDIADEDPVLGKAARLRLAPFSEDESFAAIAGFVMRATEPLASKIHARS
                     GGNPLFLEEICQTLSGEFDADLHEGEIPRNVRELIQVRLGRLEPRAASLLHVASVLGN
                     SFPIWLLREVAPELDVDALLDKLLLEGFLVEAETAEQVRFRHGIAREVVYDSVRVRER
                     RRLHAEVARVLEATFVGLARADHFEALASHHAAAGNAALAFEYAELSGDKAAASSALD
                     RARAQYGTALAQLDLLERSAELVKRRLQVVVKWAAACVFSPNREQLSVLAEASELAEQ
                     SGDQAAQMRVEYSFGWLCYALGDQRAAVEHLSRALSLARVAGDEVSEAQLLSNLGQSY
                     AASGDYDQALACLDQGIEMKRRRASKRTRTVPVGFAYALGCRGLVYGDRGEFEAAYVQ
                     VQEALSAVADSGHAVEGSLLCLLGIIQVWQGRFAEALRTAERGRLTGERVNGPYVLAI
                     CQVLSGYSEARTTGSQSALDALERAVAWLEGRDIKLYLGFVYSHFAHALLLANRSDEA
                     RHFAERAITCAATSDPLGVASGERTLAMLAARRGERDSAFSHIERAFTSARVRQSDRE
                     AALTHLTLGVIDGIFEQSDAERDQLRTAHVMFDRLGMAYHRAEAERLLMSLSRDRSER
                     TPG"
     gene            3258..3476
                     /locus_tag="VFQ35_10045"
     CDS             3258..3476
                     /locus_tag="VFQ35_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931020.1"
                     /translation="MIAPDDCPRGPDEIREPGHARSLQFLAALEILGPVLIMHQSSEL
                     ICVESRSERARRANTNEKVDLLASADDS"
     gene            3585..4322
                     /locus_tag="VFQ35_10050"
     CDS             3585..4322
                     /locus_tag="VFQ35_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9931021.1"
                     /translation="MVRVRGFCIDRYEVSTVDKRTLAPLSPYYAPDARLVEAVYQQWQ
                     VERRTVGSEPAREFALPELPIIQKSRRDYVAQAVSRPGLVPQGYLSYPLAKLVCERAG
                     KRLCTEAEWVTACKGESGRKFPYGEHFEQSQCNVYRNLHPATVLHGSASYGHRDPRLN
                     LVSEPSIGPLLRATGSTPTCASRWGNDRIYDMVGNLDEWVDEQMFLGGFYARSTREGC
                     ESKVSSHAPAYYDYSTGTRCCRAVGEP"
     gene            complement(4382..>4766)
                     /locus_tag="VFQ35_10055"
     CDS             complement(4382..>4766)
                     /locus_tag="VFQ35_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931022.1"
                     /translation="IYFLLGFATRRKRKRVLQALHTLRSIAHIVDMHQLTKDPESMLA
                     QGPRTPSSPKRTMSAFELNRYLDYSSEMLALVGKVAAVYVQEFDDPVALDAASSVQDL
                     AVNLSRSIWQKIVILERSAPESARA"
BASE COUNT          830 a         1549 c         1565 g          822 t
ORIGIN      
        1 atcgcgaggc aatggattac cccgtgcacg cttgccctgg gcagaagatg gcgttcggcg
       61 ttcttaccgg aatgttcgtc gcgatcctgt ctctccccga actcgccatc ttgccgtctc
      121 tcagcctggc gttcgctccg agcggtcgcg cgagaggctc atgagcagcc gctcggcttc
      181 ggcgcggtga tacgccatcc cgaggcgatc gaacatcacg tgcgccgttc ggagctggtc
      241 gcgctcggcg tcgctctgct cgaagatccc gtcgatgacc cctaacgtga ggtgggtgag
      301 ggccgcctcg cggtcggact gacgcacgcg cgcggacgtg aaagctcgct cgatgtgcga
      361 aaaggccgag tctcgctcgc ctcgacgagc ggccaacatc gcgagggtgc gttctcccga
      421 ggcgactccg agtggatccg aggtggcggc gcaggtgatg gcgcgctccg cgaagtgacg
      481 tgcttcgtct gaccggtttg caaggagcaa ggcgtgagcg aagtgcgaat acacgaagcc
      541 caggtagagc ttgatgtcgc ggccttcgag ccaggcgacc gcgcgctcga gcgcgtctag
      601 cgccgactgg ctgccggtcg tgcgcgcttc ggagtagccg ctcagcacct gacaaatcgc
      661 tagtacgtag gggccgttca cgcgttcgcc ggtcaagcga ccccgctcgg cggtccggag
      721 cgcctccgcg aagcgtcctt gccaaacctg gatgatgccg agcaagcaca atagggagcc
      781 ttccaccgca tgtccgctgt cggcgacggc cgagagagct tcttgcacct gcacgtaggc
      841 ggcctcgaac tcgccgcggt ctccgtaaac gagccctcgg cacccgagcg cgtacgcgaa
      901 cccgaccggt acggtgcggg tgcgcttgct cgctcgccgc cgcttcatct cgatgccttg
      961 gtcgaggcag gcgagcgcct ggtcgtaatc accagacgcc gcgtagcttt gtccgaggtt
     1021 ggatagaagc tgcgcttcgc tcacctcatc accagcgacg cgtgccagcg agagggcgcg
     1081 agagaggtgc tcgaccgccg cgcgctggtc gccgagcgcg tagcagagcc agccgaagga
     1141 atactcgacg cgcatctgcg cggcttggtc gccactttgc tcggcgagct cagacgcttc
     1201 ggccaacacc gaaagctgct ctcgattggg gctgaatacg caagcggcgg cccatttgac
     1261 gaccacttgc aaacgacgct tcacgagctc cgcgctgcgc tccaagaggt ccagttgagc
     1321 gagggcggtg ccgtattggg cccgcgcgcg gtcgagcgcg gagctcgcgg cggccttgtc
     1381 gcccgagagc tccgcgtatt cgaatgcgag agcagcgtta ccggcggcgg cgtgatgtga
     1441 cgcgagcgcc tcgaagtgat cggcgcgcgc gagaccaacg aacgtcgcct ccaagactct
     1501 ggcaacctcg gcgtggaggc gacgccgctc ccgtacccgc accgagtcgt agacgacctc
     1561 gcgcgcgatg ccgtgacgga agcgtacttg ctccgcagtt tctgcctcca cgaggaagcc
     1621 ttcgagcagc agcttgtcga gcagcgcgtc cacgtcgagc tcgggggcga cctcgcgaag
     1681 cagccagatg gggaagctgt ttcccagcac cgacgcgacg tggagcaagc ttgccgcgcg
     1741 cggctcgagt cggcccagcc gcacttgaat cagttctcgc acattccgcg ggatctcccc
     1801 ctcgtggagg tccgcgtcga actcgccgga aagggtctgg caaatctcct cgaggaacag
     1861 cgggttccca ccagaacgcg cgtgaatctt ggaggcgagc ggctcggtcg cgcgcatcac
     1921 gaagccggcg atcgcagcga acgactcgtc ctcgctgaag ggagcgagcc ggagccgcgc
     1981 ggcttttccg agcacggggt cctcgtcggc gatgtcgcgc gccatcacca cgacttgaat
     2041 cggtagctcc ccgaccgcgc gcaccaggcg acccatcacc tgaaccgaag cgtcgtcggc
     2101 ccactgccaa tcgtcgagca cgaggagcag cggtgtcgtc ttgccgagcg cgagcatgag
     2161 ctcggtaacg gcttgaatca cgtcgaggtt cgcgtccgaa gtccgttcgc gcgccctcac
     2221 cgagagtacg cccaacaacg tggtcagatg gatccgcagt ccctcgtcga tctgggtgag
     2281 ggctcgctcc acgctccaga tgctctgctc cgcgggcgcg tccttgggag ttggcaacac
     2341 ttcgcgcagg atctgcaaaa agggtcgtag cggagcgacg tcgccataag tttcgcagac
     2401 accgcggtga attctgagct ggggcaaagt ggcccgtgcg cgtcggcaga actccgagac
     2461 gagccgcgac ttgccgatgc ccgcgtcacc gagcacacag atgacgctcc cttgcccgtg
     2521 cgccgcgcgc gccagggagg cgtaaagcgc cgtgagctcg cgttcgcgac caacgaacga
     2581 tgcgcggcca cgacgtgaac gcgcaggttc gtcttccggc gcggcgccga ggatgcgcga
     2641 cgcggccacg ggatgactct tgcccttgag gaggagcgcc gggacgctct gcaccttgaa
     2701 gaaggcttct acgccgtgca gcgtcgagtc gctgacgatg acctcgtcgc ggccggcggc
     2761 cgaacacagg cgagccgcgg tgttgaccgc gtcaccactc agttcgtact gaccggccaa
     2821 agggttgccg cgacgcaaga agaccagccc cgaatgcacc cccgagtgca ggcgcgccga
     2881 aaaaccgcga agcccaggcg catcgaagcg cagctcggcg gctgcgcgat gaaattcgag
     2941 cgcggcgccg agagcgcggc ggaccgcatt ttcttccggt tccgggaacc cgaagaccgc
     3001 gagcaagccg tcgccgacga actgattcac ttgcccgccg tgcttggtca cgactcgatt
     3061 ggcgagctcg acgatgcgtg cgcgcagctc gtccgcgtcc tccggatcga gcttttcgat
     3121 gatgtgcgtg tagtcgcata catccgaaaa caagagcgtc acgtacgagc gacgttcgac
     3181 cacgtcgctc gtcgtgagcg tctcgtcacc ggactttctc atggagtttc atctttccac
     3241 gttttctcga gcgaggcttg atcgcccctg acgactgccc gcgcggcccg gacgaaattc
     3301 gcgagccagg ccatgctcgc tccttgcaat ttctcgccgc gcttgaaatt ctcggccccg
     3361 tgctgatcat gcaccaaagc tcggaactga tttgcgtcga gtcacgttcg gaacgcgccc
     3421 gtagagccaa cacgaacgaa aaggttgacc tgctcgcgag cgctgacgac tcctgaggcc
     3481 ccgtgcgcgc gatatacggc ttgttttcgg ctctcgtttt ggcaggtgcg ggcttgcttc
     3541 ccgtcgttcg ggctcgagct gccggcgcgg gctgcccttc ggacatggtt cgggttcgcg
     3601 ggttttgcat cgatcgctac gaggtctcga cggtcgacaa gcgaaccctg gctccgctct
     3661 cgccttatta cgcgcccgac gcgcgactcg tggaggctgt ctaccagcaa tggcaggtcg
     3721 agcgacgcac ggtgggctcg gaacccgcgc gcgagttcgc gttgccagag ctcccaatca
     3781 tccagaagag ccgccgcgac tacgtagctc aagccgtgtc gcgtcccggg ctcgtaccgc
     3841 aaggctactt gtcttatccg ctcgcgaagt tggtctgcga acgcgcgggc aagcgcctgt
     3901 gcaccgaagc agagtgggtg accgcctgca agggcgagtc gggccgcaag tttccgtacg
     3961 gcgagcattt cgaacagagc cagtgcaacg tctaccgcaa cctgcacccg gcaacggtcc
     4021 tacacggctc agcgtcttac gggcatcgcg acccgcggct gaatctggtg agcgagcctt
     4081 caatcggccc tctgctgcgc gcgacgggct cgaccccgac gtgcgcgagc cgctggggca
     4141 acgatcgtat ctacgacatg gtcggtaacc tcgacgaatg ggtcgacgag cagatgttcc
     4201 tcggtggatt ttacgcgcgc tcgacgcgtg agggctgcga atcgaaggtg agctcgcacg
     4261 cacccgccta ctacgactat tcgacgggaa cgcgctgctg tcgcgccgtc ggagagcctt
     4321 gagtcggtac gcgcgctgag gcggctcgcc gcgcgcatcg agagcgcatc gaagcagaat
     4381 gctacgcgcg ggccgattcc ggtgccgacc gctccagaat cacgatcttc tgccagatgg
     4441 agcgcgaaag gttgaccgcg aggtcttgca cggagctcgc cgcgtcgagc gcgacggggt
     4501 cgtcgaactc ttgcacgtag acggcggcca ccttacccac gagcgcgagc atctcgctcg
     4561 aatagtcgag gtaacggttg agctcgaagg cgctcatggt gcgtttgggg gaggagggag
     4621 tgcgtgggcc ttgggccagc atgctctccg gatctttcgt gagctggtgc atgtccacga
     4681 tgtgggcgat ggatcgcagc gtatgcagag cctgcaagac tcgctttcgc ttccggcgcg
     4741 tcgcgaagcc gagcaggaag tagatc
//
LOCUS       DASTKS010000485         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1417044, whole genome shotgun sequence.
ACCESSION   DASTKS010000485 DASTKS010000000
VERSION     DASTKS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417044"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            593..4054
                     /locus_tag="VFQ35_10060"
     CDS             593..4054
                     /locus_tag="VFQ35_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9931023.1"
                     /translation="MTEPDRRQAKHSVSAVPGESMSPPQGLLRRGDLLGRYQLLCAMA
                     RGGMGEVWAARETGRLGLPRLVAIKIPLLDQQASRGEFEQSLFDEARVAASIDHPNVC
                     KILELGRARGYLFLAMEWIHGVSLSVLRAALAPTHRMDFGMAAFITAQACGGLHAAHE
                     LKDEDGVSLDVVHRDATPHNLLIGANGDVKVVDFGIAKARNQRRQVTEAGAIKGKLSY
                     LAPEQLAGKTVDRRTDIYMLGCVLYLITTGHLAFDSRDAARTLMQISQGDYTPASSLV
                     ANYPARLEAIVNRAMAIDPDKRFRTADEMRIALETFVSEQPRPATREDLSALVTHHCG
                     KMIEERRSEVRIAQKTFDAEINGSPSEERLGDAGSPPRPRPKAPPFPVSTRKDSGVGA
                     VEVSAAPVAAAPERNVPPKPLPPRAPDAANVLARVPGTLGCSVGDRNGFALGQHGQMK
                     NSQQALVVSLPIAEQFGLVGTLFGFGGLSLVTTKSSSSTLVTVYQANHTLAVEVDSSR
                     PLTNIEFALKSTDFAGENEWVLSDKEIEYVEPDEPPKPLRPEPKPRTVPREGAHANHP
                     ANSKAGGAGPAPSLNQDWAEVRRTLIKGQLSSAQRAVMRIQRTLAKSDQSHDSIDSAQ
                     LLQPLLEAIANILAGDNQAGLKKLELVVENARISASFKWAAQVWSARASANTGDGLEA
                     ARVYAESSLRLAERLDDEALAVSTLLVAEIMNYAGERKRALELTATARALFTSLGDKQ
                     ELAACWLLEARVLAGLDREQDSIRAAQRAQESRPSWPPPVTLLVKQALAGGRVPDADQ
                     ALQALLARRPIPAEAERDRRLIEHVRAGIVPAAAACTYLDLLEARVDGENIRRLESLS
                     SKYPRVAQFLETLGWKLLRAGKAEPAKAVFERLSARGDLPDDLRASVLLGLGCLATNE
                     SGDERPSAKLRAAVNAAPKHSTKLKAATPAKPPSIRPRPPEPADFRLSHSVPPKGSEP
                     ASLNEATAQRKGLGGPVFSGSLELFSLPDLLEFMRAGRRTGTLICSSVAGIGAVHLHA
                     GNVTGAAAPETRELVEYLVLRKAVTREQLASVQPPHGGSNPEAMIGRELVRRGLITLD
                     QLRQALRDQIMAALADLARWGEGQFAFNSESGERPEATELDIEFDAQALLLEMYKEMD
                     ERATNAR"
     gene            4115..>4708
                     /locus_tag="VFQ35_10065"
     CDS             4115..>4708
                     /locus_tag="VFQ35_10065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931024.1"
                     /translation="MNDVNLIGTKLGNYRLERLLGRGRMGVVYLARDEALLRPTAVKI
                     LSWSLPEQHGLNPEDWFLAEARNVARINHPHVIQIYGVAKHAQHCYIAMEYVAGAAAD
                     AWVSETGPFSPLRATEILLQAANALQAAHDADVVHRDVKPGNLLITADGTAKLGDFGM
                     ALTVTGDARFEATRAGTPYYTAPEIWRGQSATRSTDIY"
BASE COUNT          829 a         1533 c         1572 g          774 t
ORIGIN      
        1 aagatcctgc ggctgcgaag cacggcctcg tcgctccgct cctcgaaagg cttcagcctt
       61 cctccggtgc tcactcctcg ggcgcgcttc tcgctcgcgt ttcttggctc tcgcgacggg
      121 caggaacttg ggacaccccc ctaatccggt gccctccgcc gacgtaggcg acgagcgttt
      181 gcggagggct ccgaacgggt ccgcgtaggt gggcgaagca ggtgacgcag cgtgagcttt
      241 ttcggcgctg cggttgcgct tcgccactgc gtgcggctcg ggggagagtc tggctcgctc
      301 gcgactgacg tatcgattcg cggatcctat cgagacgagc gagtcactcg gggagccaat
      361 tcgggaaatg ggattctcaa ttcgacgctc tggcgaggct cgatgcagcg atatttttgc
      421 gtgcgcaatt ttcgcgtcga cccgtcgtca tcgagagaaa aatcgacgct gatgatcgct
      481 taagcacggc cggtggaaac cgtatggggc gttggttctc gcatttgtac gagatgcctg
      541 cctcaagaac tcctcagagt gaaacccact ccgcggattg ggatgctgtg ccgtgaccga
      601 accggataga cgccaagcca agcactcggt gagcgctgta ccgggggaat ccatgtcgcc
      661 tccgcaaggg ctgctgcggc ggggtgacct actcggccgc tatcagctct tgtgcgccat
      721 ggctcgaggg ggaatgggag aggtatgggc ggcgcgcgag acgggccggc tcgggctgcc
      781 gcgcctagtc gcgatcaaga ttccgctgct cgaccagcag gcgtcacgcg gggaattcga
      841 gcagtcgttg ttcgacgaag cgcgcgtggc cgcgtccatc gatcacccga acgtgtgcaa
      901 gatcctcgag ctcgggcgcg cgcgcggcta cctgttcttg gccatggagt ggattcacgg
      961 ggtgtcgctg tcggtgctac gcgcggcgct cgccccgacg caccgcatgg actttggcat
     1021 ggccgcgttc atcactgcgc aggcgtgcgg gggattgcac gccgcgcacg agctgaagga
     1081 cgaggacggc gtctcgctgg acgtcgtgca tcgcgacgcc acgcctcaca acctcctgat
     1141 cggcgcgaac ggcgacgtca aggtcgtcga tttcggcatc gccaaggcgc gcaatcaaag
     1201 gcgccaggtc accgaggccg gggcgatcaa gggcaagctc tcgtaccttg cgccggagca
     1261 gctcgccggc aagaccgtcg atcgtcgcac cgatatctac atgctcgggt gcgtcctcta
     1321 tttgattacc actggccacc tcgcgttcga ttcgcgcgac gcggcgcgaa cgctgatgca
     1381 aatctcgcag ggcgattaca cgcccgcgtc gtcgctcgtg gcgaactacc ctgcgcggct
     1441 cgaagcgatc gtcaatcggg ccatggcgat cgatccggac aagcgctttc gcactgccga
     1501 tgaaatgcgc atcgcactcg agacgttcgt gagcgagcag cctcgccccg cgactcgcga
     1561 ggacctctcg gcgctcgtca cccaccattg cggcaagatg atcgaggagc ggcgctccga
     1621 ggtgcgcatc gcgcagaaga cgttcgacgc cgaaatcaac ggctcacctt cggaagagag
     1681 gctcggcgat gctggttctc cgccgcggcc aaggcccaag gcgccgccgt tcccggtgag
     1741 cacccgcaag gactccgggg tcggcgcggt cgaggtctcg gcggctcccg tcgcggccgc
     1801 gccggagcga aacgtgccgc cgaagcctct ccccccgcgt gcgcccgatg ccgcgaacgt
     1861 gctcgctcgc gttcccggca cgctcggctg ctcggtcggg gaccgtaacg gcttcgctct
     1921 tggccagcat gggcaaatga agaactccca gcaggcgctc gtcgtctcgc ttccgatcgc
     1981 ggagcagttc ggcctggtgg gcacgctgtt cggttttggg gggctgagcc tcgtgaccac
     2041 caagagctca tcgtcgacgt tggtcacggt gtatcaggca aatcacacgc tcgcggtcga
     2101 agtcgactcg agtcgcccgc tcacgaacat cgagtttgcg ctgaagagca ccgatttcgc
     2161 gggtgagaac gagtgggtgc tgtcggacaa agagatcgag tacgtagagc ccgacgaacc
     2221 gccgaagcct ttgcgccccg agccgaaacc ccgcacggtg ccgcgggaag gggcgcacgc
     2281 aaaccaccca gccaactcca aagccggcgg cgcggggccg gcgccctcgc tcaatcagga
     2341 ttgggcggag gtgaggcgaa ctctgattaa gggacagctc tcgtcggccc agcgggcagt
     2401 gatgcgaata caacgcacgc tggcgaagag cgaccaatcg cacgactcga tcgatagcgc
     2461 gcagctgcta cagccgctcc tcgaggcgat cgcgaatatc ctggccggcg acaaccaagc
     2521 gggcctgaag aagctcgagc tcgtggtcga gaatgcgcgc atcagcgcgt cgttcaagtg
     2581 ggcggcgcag gtgtggagtg cgcgcgctag cgccaacacg ggtgatgggc tcgaggcggc
     2641 gcgggtgtac gccgagtctt cgctgcgact ggccgagcga ctcgacgacg aggctttggc
     2701 cgtcagcact ttgctcgtgg ccgaaataat gaactacgcc ggcgagcgaa agcgcgcgct
     2761 ggagctcact gccaccgcgc gggcgttgtt cacgtcgctc ggcgacaagc aagagctcgc
     2821 cgcgtgctgg ctgctcgagg cgcgcgtgct cgcggggctc gatcgagagc aagacagcat
     2881 ccgcgcggct caacgcgcgc aggagagccg gccatcgtgg ccgcctccgg tgacgctact
     2941 ggtgaagcag gcgctcgccg gcggtcgcgt gccggacgcc gatcaagcgc tccaggcgct
     3001 cctcgctcgt cggcccatac ccgcggaggc ggagcgcgat cggcgtctca tcgagcacgt
     3061 gcgggccggc atcgttcccg cggcggcggc ctgtacttac ctcgacctgc tcgaagctcg
     3121 cgtcgacggc gagaacatcc gacgactcga gagcctttcg tcgaagtacc cgcgcgtggc
     3181 gcagttcctc gagacgctcg ggtggaagct gcttcgcgcg ggcaaagccg aacctgccaa
     3241 ggccgtcttc gaacgactca gcgcgcgcgg cgatctgccc gacgacctcc gcgcgtcggt
     3301 gctgctcggc ttgggctgcc tcgcgacgaa cgagagcgga gacgaacgcc cgagcgcgaa
     3361 gctacgagca gcggtgaacg cggcgcccaa gcactcgacc aagctgaaag cagcgacgcc
     3421 ggccaagccg ccgtccatcc gccctcggcc gccggaaccg gcagattttc ggctgagtca
     3481 ttcggtcccg ccgaaagggt cggagcccgc ttcattgaac gaggcgaccg cgcagcgcaa
     3541 gggcctcggc ggcccggtgt tctcggggag cctcgagctc ttctctctcc ccgacttgct
     3601 cgagttcatg cgggcggggc gacgcaccgg cactctgatt tgcagttcgg ttgcaggcat
     3661 cggcgccgtc cacctgcacg ctggcaacgt gacgggcgcc gccgctcccg agacgagaga
     3721 gctcgtcgag tacttggtgc tgcgcaaggc ggtcacgcgc gagcagctcg ccagcgtgca
     3781 gcctccgcac ggtggttcca acccagaagc catgatcggc cgcgagctgg ttcgtcgtgg
     3841 actcatcacg ctcgaccagc tgcgccaggc gctacgcgat caaatcatgg ctgccctggc
     3901 cgatctcgcc cgttggggag agggtcagtt tgcattcaac tcggagtcgg gagagcggcc
     3961 cgaggcgacg gagctcgaca tcgaattcga tgcccaagcg ctgctcctcg agatgtacaa
     4021 agagatggac gagagagcta cgaacgcaag ataagatgcc ggaaggcgcc gcgaagcagc
     4081 gttgagatct cgcgcgccgg cccaggtcga atcagtgaac gacgtaaacc tcataggtac
     4141 gaagctcggc aactaccgac tggagcgctt gctcggtcgg ggccgcatgg gcgtcgttta
     4201 cctcgcgcgc gacgaggccc tgctacgtcc gaccgcggtg aagatccttt cgtggtccct
     4261 acccgaacag catggcctga accccgaaga ctggtttttg gccgaggcgc gcaacgtggc
     4321 gcgcatcaat cacccccacg tgattcagat ctacggggtc gcgaagcacg cgcagcactg
     4381 ttacatcgca atggagtacg tggccggcgc cgccgcggac gcgtgggttt ccgagacggg
     4441 cccgttctct ccgctgcgcg cgaccgaaat tttgcttcaa gcggcgaatg cgctgcaagc
     4501 cgcgcacgac gccgatgtcg tgcaccgcga cgtgaagccc ggaaacctac tgattacggc
     4561 cgacggcacc gcgaagctcg gggacttcgg tatggcgcta acggtcaccg gagacgcgcg
     4621 cttcgaggcc acccgcgccg gaacgccgta ttacacggcg ccggagatct ggcgcggtca
     4681 gtccgcgact cgttcgaccg acatctac
//
LOCUS       DASTKS010000486         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_745954, whole genome shotgun sequence.
ACCESSION   DASTKS010000486 DASTKS010000000
VERSION     DASTKS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745954"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFQ35_10070"
     CDS             complement(<1..441)
                     /locus_tag="VFQ35_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931025.1"
                     /translation="MPSQRSDPPRAFNRAWRCAALGLASLLFAPRAFAQSAPDPSAKA
                     FRVVFSSSTSCSDPTEFAARLREMTTRWRPASNGEPSYTFFVHLSATQAGAHGQLGVL
                     EPSGALLLRELETADCHTLITALAFIGAVMADPFAAPPLEKVPAR"
     gene            748..1686
                     /locus_tag="VFQ35_10075"
     CDS             748..1686
                     /locus_tag="VFQ35_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931026.1"
                     /translation="MQLQRGYELKQSGRCADAIPYFVESLRLDRQPKALIHLADCERQ
                     IGKYVSAEEHLVAARDLGRELSLDGFVKLAESRLAELESHLPKLTLRLAAGVPGDAVV
                     TRDGVTLAPLSLGVALPVDPGAHEVRVRGGGLERRYSVELRDGESKELQVTTAGGEPV
                     ASPKQEPPVVPAVAPSQPPPSNAAAPLAPATQRSSRPDVQRTFGFAVAGAGIIGLGVG
                     SIFGLRTRSKNDDADGMCTTSSPCDAAGRARYDETVEDARHARTISFAAFATGGALLA
                     VGGVLIFTGSARREGALRLAPSVAQNSFGAVAEGVW"
     gene            1686..3155
                     /locus_tag="VFQ35_10080"
     CDS             1686..3155
                     /locus_tag="VFQ35_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9931027.1"
                     /translation="MTTKARWRLARRHFALQSAVLGSICSTFANCSIHEHEPVLSDAG
                     ATNGGQLGDSGKGGAAGKSGSGGQSASAGEAGRANGGATSGGATAAGEGGVAGAPDAG
                     TGASGENAGAGGLGGDTGAGGTATGRGGTSAEGGRSNGGASGSGGAKGGATGTGGAGG
                     TVGGNGNGGNGNGNGGNGTGGKGTGGNGGATSCAAKDVGPEGASCAGSSNVCGPGESC
                     CTSLCVPGGLFTLGGNVESRKSQARVSGFYLDKYEVSVGRFRKFVAVYDAWLFDKNPR
                     DGAAKHPLVAGSGWQATAWASSLPASSDKLVENITTNCSPQHDGAYSTWSASGANDGL
                     PINCLDWFEAFAFCAWDGGRLPTEAEWEYAAAGGDQGYVYPWGPQPPTSEYASYESDM
                     TVPVGSKPKGAGRFGHLDLAGSVEEWVLDLYSTPYPSTMNDYAFVQTDPQHFLRVFRG
                     SAWGQPAEELNVAIRRNEEPTARGSYIGVRCARPSATTP"
     gene            complement(3257..>4538)
                     /locus_tag="VFQ35_10085"
     CDS             complement(3257..>4538)
                     /locus_tag="VFQ35_10085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931028.1"
                     /translation="ARIVGALRSEHVTRVYELGQLENGVHYMAMEYLDGADLSALLAQ
                     HGRFHFTEAASIVRQACEAIAEAHQSGVVHRDLKPANLFSTRRPNGAQLVKVLDFGIS
                     KTSTGSAADHSMTRTSAIMGSPYYMSPEQLARPKTVDFRTDIWSLGVILHELVTGRVP
                     FQGDTLPELTLNVHHDEPPSIATFSPDVPAAYQAIVTRCLQKDRELRYGSIRELSDAL
                     AACESVHSLSGLTETALGGEGSVSRTALEAAATHAALAAKAPQPGKTGNWGGTIPNGR
                     RPRRVFAIVGSALGVAALGSTALVISLRGRGAQTEEPNAPSLAASASAVARAQHVVAQ
                     PLPSSSEAPDSPSRTAAPAPANAHAPEAPSTVASNNPIPLPLSGPSREAPPTRKPRAA
                     DPRPSASTSPKSSRGDGAQSGQHPAGWDDEILKP"
BASE COUNT          780 a         1440 c         1602 g          716 t
ORIGIN      
        1 acgcgcgggg accttctcga gcggtggagc ggcgaacggg tctgccatca ccgccccgat
       61 gaaggcgagc gcggtgatca acgtgtggca gtcggccgtc tcgagctccc gcaggaggag
      121 agcgccgctc ggctcgagca caccgagctg cccatgcgcg cctgcctgcg tcgcgctgag
      181 atgtacgaaa aaggtgtacg aaggctcgcc gttcgaggct ggtcgccagc gcgtcgtcat
      241 ctcgcgcaga cgggccgcga attcggtcgg atccgagcag ctcgtgctcg agctgaatac
      301 gacgcgaaaa gcctttgcgc tcgggtccgg cgcgctctgg gcaaacgccc gaggcgcgaa
      361 cagcaggctt gccaaaccca gtgccgcgca ccgccacgcg cgattgaagg cacgaggcgg
      421 atcgctacgc tgcgaaggca taccggcgct caaggtagta aaccgttcac ggtcccatac
      481 caaggaacga gcgccgagcg aagctcaagc tacggcacgc cgcgaaaatc tgcactttgt
      541 tcgcgtgccc cgggcggagc cgagacgaga gctcccccgc cctcggagcc ggggctctac
      601 gcgctcgaag cgcggccgaa catgataagc tcagcaaccg aaatgcggcc ttttgtgctt
      661 ctacggatac gctcggcgct cgcatctgca gcgctcgtcg tctcgtacag caccgcgtcc
      721 ttcgcatctg accctggcgc ggcgcgggtg cagttgcagc gcgggtacga gctcaagcaa
      781 agcggccggt gcgcagatgc gattccgtac ttcgtcgaat cgctccgact cgaccgccag
      841 ccgaaagcgc tcatccacct cgcggactgt gaacgacaga taggaaagta cgtttccgcc
      901 gaggagcacc tggtcgccgc gcgcgatctg ggtcgcgaac tttcgctcga cgggttcgtg
      961 aagctcgccg aaagccgctt ggccgagctc gagtcgcatt tgccgaagct cacgctgcgc
     1021 ttggccgcgg gcgttccggg ggacgcggtg gtgactcgag acggcgtgac tctggctccg
     1081 ctttcgctcg gggtggcgct gccggtcgac cccggagcgc acgaagttcg ggtacgcggc
     1141 ggcgggctcg agcggcgcta ctcggtagag ctgcgagacg gcgagagcaa agagctccag
     1201 gtgaccaccg cggggggcga gccggtcgcg tctccaaagc aggagcctcc cgtcgtgccc
     1261 gcagtggcgc cctcgcaacc gcctccttcg aacgccgccg cgccactcgc accggcaacc
     1321 caacgctcct cgcgccctga cgttcagcgc acgtttggct tcgcggtggc gggagccggc
     1381 atcatcgggc tgggcgttgg ctcgatcttc ggcctgagaa cgcgctcgaa gaacgacgat
     1441 gccgacggaa tgtgcacgac gagctcaccg tgcgacgccg cggggcgcgc tcgctatgac
     1501 gagaccgtcg aagacgcgag gcacgcgcgc accatctcct ttgccgcgtt cgcaaccggt
     1561 ggggcactgc tcgcagttgg cggggtcttg atcttcacgg ggtcggcccg gcgcgaaggg
     1621 gcgctgcgtt tggcgccgag cgttgcgcag aattcgttcg gtgccgtcgc cgaaggagtt
     1681 tggtagtgac gaccaaggcg cgttggcggc tcgcccggcg gcatttcgcg ctgcagagcg
     1741 ccgtgctcgg ttcgatttgt tcgaccttcg ccaactgctc gattcacgag cacgaacccg
     1801 tcctcagcga cgcgggcgcg acgaacggcg gacagctcgg cgatagcggg aaaggcggag
     1861 ccgcgggtaa gagcggcagt ggtgggcagt ctgcgagcgc cggcgaggct ggccgagcaa
     1921 acgggggagc aacgtcgggc ggagccacag cggccggcga aggaggcgtt gccggcgctc
     1981 ccgacgctgg aacaggcgcg agcggagaga atgccggcgc gggcggcctt ggcggagaca
     2041 ccggcgcggg cggcacggcg accgggcggg gcggaaccag tgctgaaggc ggtcgaagca
     2101 acggcggagc gagtggcagc ggcggcgcca agggtggcgc aaccggcact ggcggagcgg
     2161 gcgggaccgt cggcggcaac ggcaacggcg gcaacggcaa cggcaacggc ggcaacggta
     2221 cgggcggcaa gggcaccggc ggtaacggcg gtgccacgag ctgcgcggcg aaggacgtcg
     2281 gtcccgaagg cgcgtcgtgc gcgggctcgt cgaatgtctg cggaccggga gaaagttgtt
     2341 gcacgagcct gtgcgtaccg ggtgggctct tcacgctcgg cggcaacgtc gagtcgcgaa
     2401 agtcgcaggc gagagtgtct ggcttttacc tcgacaagta cgaggtcagc gtcgggcgct
     2461 ttcgcaagtt cgtggctgtc tacgacgcct ggctcttcga caagaacccg cgagacggcg
     2521 cggcgaagca tcctctcgtc gctggcagcg gctggcaagc aaccgcgtgg gcttcgtctt
     2581 tgcccgcgag ctcggacaag ctcgtcgaaa acatcaccac gaactgcagc ccgcagcacg
     2641 acggcgcgta cagcacttgg tcagcgagcg gcgccaacga cgggctaccg ataaactgcc
     2701 tcgattggtt cgaagcgttc gcgttctgcg cgtgggacgg cggcagactg ccgaccgaag
     2761 ccgagtggga atacgccgcc gcaggcggtg accagggcta cgtgtatccc tggggcccac
     2821 aaccgccaac tagtgagtac gcgagctacg aatccgacat gaccgtcccg gtcggctcga
     2881 agccgaaggg agcaggccgc tttggtcatc tcgacttagc gggctccgtg gaggagtggg
     2941 ttctcgactt gtacagcacc ccctatccct ccacgatgaa cgactatgcg ttcgtgcaaa
     3001 ccgatcctca gcacttcttg agggtctttc gcggcagcgc gtggggacag ccggcggaag
     3061 aattgaacgt cgcgattcgc cgcaacgagg aacccaccgc tcgcgggagc tacatcggcg
     3121 tgcgctgcgc gagaccgagc gcgacgacac catgactcga cgccgaactt cgccaagggt
     3181 ccgcgaatgg ctaagtcagt cgcgagcggg gcgagaccgt gtatcatccc gagcgccgct
     3241 gttgtccaac acccggttag ggcttcaaga tctcgtcatc ccagcctgcg ggatgctgcc
     3301 cggattgcgc gccgtcgcct cggctcgact tgggagacgt actggctgac gggcgggggt
     3361 cagcggcgcg cggtttccga gtgggcgggg cttctctcga agggcccgaa aggggcagtg
     3421 ggattgggtt gttcgacgcg accgtgctcg gtgcttctgg cgcgtgagcg ttcgctggcg
     3481 cgggcgcagc agtgcgcgag ggcgaatcag gcgcttcgct cgagctcggc agaggctgcg
     3541 cgacgacgtg ctgcgctcgt gcgaccgccg acgcgctcgc ggcgagactc ggcgcattgg
     3601 gttcttctgt ttgggcgcct cgaccgcgca gcgagatcac gagagccgtc gagccgagtg
     3661 cagcgacgcc cagcgcggaa cccacgatcg caaagaccct gcgcgggcgg cgcccattag
     3721 gaatcgtgcc gccccagttt ccggtcttgc caggctgcgg agctttggcg gcgagcgcgg
     3781 cgtgggtcgc ggcggcctcg agtgccgtac gacttacgga gccctcgcct ccgagcgcgg
     3841 tctccgtcag ccccgaaagt gaatgcacgc tttcacaggc ggcgagcgcg tcggacaact
     3901 cacggatcga accgtagcgg agctcgcgat ccttctgtaa gcagcgggtg acgatcgctt
     3961 gatatgcagc cggcacgtcc ggactgaagg ttgcgatcga cggcggctca tcgtggtgca
     4021 cgttgagcgt gagctcgggt agcgtgtcgc cctgaaacgg aactctgccg gtgacgagct
     4081 cgtgcaaaat cacgccgagc gaccaaatgt cggtgcgaaa gtccaccgtt tttggccgcg
     4141 ccaattgctc tggcgacatg tagtacggtg aacccatgat cgcgctggtc cgggtcatgc
     4201 tgtggtcggc ggcgctgccg gtagaggtct tggagatgcc aaagtccaag accttgacca
     4261 attgagcacc gttcgggcgg cgcgtactga acaggttcgc gggcttcaga tcacgatgga
     4321 ccacgcccga ttgatgcgcc tccgcgatcg cttcgcaggc ctgacgcacg atgctcgcgg
     4381 cttcggtgaa gtggaaccgc ccgtgctgcg cgagcaacgc cgacaaatcg gcaccgtcga
     4441 ggtactccat tgccatgtag tgcacgccgt tttcgagctg ccccagctcg tagactcgcg
     4501 tcacgtgctc gctgcgtagc gcgccgacga tgcgcgcc
//
LOCUS       DASTKS010000487        12387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1863281, whole genome shotgun sequence.
ACCESSION   DASTKS010000487 DASTKS010000000
VERSION     DASTKS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863281"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFQ35_10090"
     CDS             complement(<1..814)
                     /locus_tag="VFQ35_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931029.1"
                     /translation="MNVQVLIDSIVRQVTVLIAQLATSGGIRAPVAHLANQVFLDLAR
                     ELEAQGVSRKVSADMFGMALRAYIRKVRRLDEAASARGRTLWQSVLEFVREEELVTRA
                     RALERFELDGELEVSSVLHDLTESGLVFCSGSGRNAVYRAATEAELGRLSQLSNDAGL
                     PELAWVFTFRHGPLKAEKLAELLSRRPEEIESVIARLIADGRVERAPDGNLTSKDFVV
                     PLGSAVGFEAAVFDHFQAVVQTICQRLNQSSVGAHGADVIGGSTYSFDVWPGH"
     gene            966..2933
                     /locus_tag="VFQ35_10095"
     CDS             966..2933
                     /locus_tag="VFQ35_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931030.1"
                     /translation="MVVHVRRLAGVAVLFGVSACGSASRNPAEHAGEGGGGTGAAGGP
                     GAAKGGNGASGGNLGAGGSGGASSAGTVGGGARGLGGATGSVGGIGATGGTAGAGAFG
                     AIGGDAASGGNGPSGGGGGAAPTGCSGPNIDVPVFVVSGTVTNNVGALKADLSGLAFR
                     NGSERLPVTPGTTTGAYSVRLVPGTYDIFYGELSPLRLGVTVGPSSPTTLDLEAKLVQ
                     VSVTASLNGGPFGSSKYATLALKNGHGTQAIGDAAEAPLSIVVAAGSYDLIYDGSRAY
                     GSAPRNSGAKLQTVVTGASDTALNVDIRSVVLSGRVRRGGTAVSGSALGVVAGTLTSA
                     ALGQVPLAVNDAGEYSVHVLPGTYDFETTGTSVTGLVVPPGTNTQIDIDLPEAPTYSA
                     TVQGSVKFNGQPVLGGWGFVFYETASEVERSIAGSGPGYSLSMLPGTYDIFYGAQRDN
                     SPETLLLPRNSRAPLQRGVRVKSSATTTLDLDAPVGVLRGKFTIGGAPLPDVSGDGSL
                     WVRGASGDEVQLASNYDGDYSFPLIAGDYDVYFKVGLFGSVAPRNAAANIARINLTRG
                     DNAIDIDVPTRHIQGTLTVSGVTVGATNDVAKITLQGPNGDTVPLGSASAKTYSTTVV
                     PGTYDIYFSGSTRNTFAPMNQSAKIGCLKVP"
     gene            complement(2991..3995)
                     /locus_tag="VFQ35_10100"
     CDS             complement(2991..3995)
                     /locus_tag="VFQ35_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931031.1"
                     /translation="MSRIRAALWAAIALSVAACRGSTTTDPDEAAGSGGAAIAGNGGN
                     ASGNGAGTTGGIAGSSATGGGGTAASTSAGRSGAQTGGMSAGSGGVGAGRSGAGAGGT
                     TIVGVAGAAGAALAGAAGANSAGHCTTAKDCDPGTLPGSLLYFCRTPFDPPLPEFVGC
                     GTKEWCGECGCAAMPDASATCTSDTQCPNERPRCVSVGHSPGITLSECSECQANSDCP
                     AEHPFCVSPGPGSARHCMDCVYTSDCSRGTCAQNRCVECTPATDCVDPVLKCSDRTGR
                     CDKIPCSLDGDCPSGSKCNAGACERTHCSLDPDCGPGYCINGACYGSPGSCATQRVLY
                     "
     gene            4222..5211
                     /locus_tag="VFQ35_10105"
     CDS             4222..5211
                     /locus_tag="VFQ35_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9931032.1"
                     /translation="MLGDLKRTLQESLFELPPRVAPAEPRVEYAEIAERLPPTVRLGT
                     MSWAFEGWRGIVYSSTSEAKRLSHEGLPAYSAHPLLRAVEIDRSFYEPLPAAAFTHFA
                     SQVPPDFRFVVKAHEETTLLRFPDHARYGKKRGTSNSRFLEASYATDQVVGPASEGLG
                     EKLGTLLFQFPPQSLGEPRRFADALESFFAGLPRGIPYTVEIRNAELLTAAYGAALAR
                     SGVMHCHNVWGPMPDVREQARQLPRDVRQRLIVRWLLRPGDTYEGARSRFLPFNRLVE
                     EDGTTRASIVELVARAVSFDVPTLVLVNNKAEGSAPLSLFRIAEALVARSPQN"
     gene            complement(5202..5753)
                     /locus_tag="VFQ35_10110"
     CDS             complement(5202..5753)
                     /locus_tag="VFQ35_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931033.1"
                     /translation="MKRLLVGLVAACSLTLLGTGAAHAEESPTAGHASAGALLGYGFK
                     DGVGLGLGVRGGYTLPQNVYLGGTFVYHLGKSESTPLGDASVHLYYFGVEGGYDIYAA
                     PVVIRPYVGLGAATATASIPGGSFGGVTIPEQTSSDTKFGVWPGATVMYPIGKAFVGA
                     DARFLIVSDFNTFSLFGTGGIQF"
     gene            complement(5896..6774)
                     /locus_tag="VFQ35_10115"
     CDS             complement(5896..6774)
                     /locus_tag="VFQ35_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9931034.1"
                     /translation="MDLLSHMEIFVRIVEAGSLSGAARSARLSLPAVSRQLSALEKQL
                     GTALIVRSTRRLSVTDAGRTFYDHAKVALREAEHAMAAARTAPSIAGRIRVSVPFAFG
                     MQHVVPRLEQLVKRHPGLHVDLHMEDEPVDLIAHGVDIAIRAGLAPPDSTDYVAHPML
                     TIRRMVVATPSYLKQHGVPKEPSELANHVCFTTLGKSSGSPWRFVRDGRETAVDVRGP
                     VRASTPAAIAALVRAGLGVAYLPDWVVAEDIASGRLRQLLAGWAAPGIGTWLVHRAEH
                     RKAARIRAFVELMTSG"
     gene            7007..7735
                     /locus_tag="VFQ35_10120"
     CDS             7007..7735
                     /locus_tag="VFQ35_10120"
                     /inference="COORDINATES: protein motif:HMM:NF006118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931035.1"
                     /translation="MNIQGKNVLVTGSTRGIGRALVEAALARGAARVYATARNRAALE
                     SSELRRDARVVPLELDITSPEQAQRAAQLAKDVDVLINNAGVLASFGVLSGTAADVQR
                     DLDTNFFGLLHLARAFAPVLAERKEAALVSVLSVASLASMPALGGYSSSKAAAWSLTQ
                     ALRAELGKRGVKTFAVFPGPVDTDMIRSFELPKTAPALVARAILDGVEASVLDIFPDP
                     MSREVGALFAKDPMAVERQFATPM"
     gene            complement(7885..8799)
                     /locus_tag="VFQ35_10125"
     CDS             complement(7885..8799)
                     /locus_tag="VFQ35_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931036.1"
                     /translation="MACDISGLAVLFVTLERARAWVESERFAEAAALLVDELPCIDES
                     QDVGLRVRARSLYGDALAGSGEAARAERELQSASRLWGSELALDWIRGLPTGETSQRD
                     VRRAAAAAARASLHVADLRLRALELAPPVFVRVAGSAERQRRAFGRYLTVEFAPWAHR
                     QYAAYEIAQRHFEQVYLSPPVAAPEWRVAVAARIGSLWGRLSDQMARVDQLCGPACDD
                     RGASYHGTFDDPWEPERQRARAAFETCITLSRKYRLLTDDTLTCERWLGSHFRTQYSA
                     LDELVPTAHWGVDAASGYALAALPANRR"
     gene            8912..10039
                     /locus_tag="VFQ35_10130"
     CDS             8912..10039
                     /locus_tag="VFQ35_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931037.1"
                     /translation="MLAVACGTPPKSRSPEPELSDVAPLSDFEPTRYRSGAPVASPIE
                     PAVSTATAVACRFEVPMWSGPLQITRANEPFATVADAHDVRIAVFETDVERGASVELD
                     VLGVTLEALVPLSELELYTTRARLFDGFVWALPGASLRLERASAGRVAAKLRLPAAIE
                     PASPSSPRELACAELGAAPANDSGQLPRELFGSRRVVQSAPFRGDRRVPLSSKPSGEP
                     IAFLDTRPSDPESLPDEAIVLERRRDQVRIAYLMDALIVFGWVPAHALQAELVETPIE
                     LVQGLAPWGRPVEPFGGDDPTRVELPGEPRTCAWNAPLAIEVAGAVRTVGTLASAIPL
                     LPLATRDGWREVEFSHPALSFSKRARAWVPERRLYPCTPRH"
     gene            10108..10488
                     /locus_tag="VFQ35_10135"
     CDS             10108..10488
                     /locus_tag="VFQ35_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931038.1"
                     /translation="MKARALVLLCGCVAVSCLATPRPQDAPAPRDYFHQAKAPSSTCE
                     PRVEVLRSAAEALRPYREVATLSASCYPGTPRLCEDRLAQRACELKADAVILMEPTSG
                     GTPPGSSGQSEISMSGRAVRWNSE"
     gene            complement(10547..12133)
                     /locus_tag="VFQ35_10140"
     CDS             complement(10547..12133)
                     /locus_tag="VFQ35_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9931039.1"
                     /translation="MMRQGRQFSSLGFVLAAAAGALACGNSASDGGHETGGTGGAITG
                     GRSSSASGGLHSGAGGTGTGGLASNHGGATSGAGGIAGGAGGTTNGSTTKGGTGGTAD
                     STAPSGGSGASSGAKNGGTGGVPSGDGGKTLGPGGESAVTGGLHGGGTGGIGGGNGTT
                     DANDFFALAVNYPLPKDAVSVQIADLDGDGTRDLIVATQVIAGSSTDWSLNVLHNKGK
                     GTFEAGDSYAQTTALGAFIAGDFNGDGAVDVITSNSNGGALHFLQNLGNGKLKASVDL
                     TVTTTNSFILSADLNGDHKLDLIFVRDTKIAFALNVGAGAFAEPQPICDRAEVDGVAA
                     GDLTGDGSADVVTRVHPEALKAFITSPSGVFAAPVSYSNPTPSGVASVLLADLDGDAR
                     ADVLTYTPGKDSGPGFASVLLNRGDGTLTSAQTYDSGAMLDVPTTAGVAGTGPIAAAT
                     GDLDGDGKLDLLFANPGGRNTSGNFGVRLNAGGGKFPNLNTYSVGAYPNQIFAAELSG
                     DKKADVVFVDHGKLFVRLTK"
BASE COUNT         2033 a         4143 c         4072 g         2139 t
ORIGIN      
        1 ggtgcccggg ccaaacgtcg aacgaatagg tgcttccacc aatcacgtcg gcgccgtgag
       61 caccgaccga cgactggttc agtcgttggc aaatggtctg caccactgcc tggaaatgat
      121 cgaacacggc cgcctcgaag ccgaccgccg aaccgagcgg gacgacgaag tctttcgacg
      181 tcaagttacc gtccggcgct cgctccacgc gtccgtccgc gatgagccgc gcgatcacgc
      241 tctcgatttc ttctggtcgc cgcgacagaa gctcggcgag cttctccgcc ttcagcgggc
      301 cgtgtctgaa cgtaaacacc caggcgagct cgggcagtcc ggcgtcgttc gagagctgcg
      361 agagccgtcc gagctcggcc tcggtggcgg cgcggtacac ggcgttgcgt ccgctcccgg
      421 agcagaacac gagaccgctc tcggtgaggt cgtgcagcac cgacgagacc tcgagctcgc
      481 cgtcgagctc gaaccgctcg agagcacgcg ctcgcgtcac gagctcttct tcgcgcacga
      541 actcgagcac cgactgccac aacgttcggc cccgagccga cgccgcctcg tcgaggcgac
      601 gcactttgcg gatgtacgcg cgaagagcca tgccgaacat gtcggcgctc accttgcgac
      661 tcacgccttg cgcttcgagc tcgcgcgcca agtcgaggaa cacctgattg gccaggtgcg
      721 ctacgggcgc tcgaatacca cccgacgtcg cgagttgggc aatgagcacc gtgacttgtc
      781 gcacgatgga gtcaatcaga acttgcacat tcatggctaa ccgccgaggg ctagaatgtc
      841 cttcatggct cgcgactggt agatgacccg agggtcatcc gcgcatggcc tggcgaccgg
      901 agctcgagcc aagattttgc tggctttatg gatcaagcgc tcacgagcgg gcatccatca
      961 gggccatggt tgtgcatgtg cgtcgtctgg cgggggttgc ggtgcttttc ggcgtgtctg
     1021 cgtgcggcag cgcctctcga aaccccgctg aacatgcggg tgagggtggg ggaggaacag
     1081 gcgcggcggg cggtccgggc gcggcgaagg gcggaaatgg cgcgagtggc ggcaacctgg
     1141 gcgctggcgg cagcggggga gcgagcagcg ccgggaccgt gggaggcggc gcgcgcggcc
     1201 tgggcggcgc aaccggcagc gtcggcggca ttggcgcgac gggcggaacc gccggagccg
     1261 gggctttcgg cgcaatcggg ggagatgccg cgtcgggcgg caatggtccg agcggcggag
     1321 gcggtggggc ggcgccgacc gggtgcagcg gaccgaatat cgacgtcccg gtgttcgtcg
     1381 tgagcggcac ggtcacgaac aacgtcggcg ctttgaaagc agatctcagc gggctcgcct
     1441 ttcgcaacgg aagcgaacgc ttaccagtca cgccgggaac gaccaccggc gcgtattcgg
     1501 tgcgcctcgt tcccggcacc tacgacatct tctacggcga gctttcgccg cttcgcctcg
     1561 gcgtcacggt gggaccatcg agcccgacga cgctcgacct cgaagcgaag ttggtgcagg
     1621 tgtcagtgac ggcctcgctc aacggcgggc cctttggctc gagcaagtac gcaacgctcg
     1681 cactgaagaa cggccacgga acgcaagcaa tcggtgacgc tgccgaagcg ccgctttcaa
     1741 tcgtcgttgc cgcgggcagc tacgacctca tctacgatgg ttcgagggcc tacggctcgg
     1801 cgccgcgtaa ctccggcgcc aagctccaaa ccgtcgttac cggtgcgagc gatacggcgc
     1861 tcaacgtcga catccggtcg gtggtactca gcggtcgcgt ccggcgcggc ggaacggccg
     1921 tgtcgggctc ggctctcggt gtcgttgccg gtactctgac ttcggctgcg ctcggccaag
     1981 tgccgctcgc cgtcaatgat gcgggcgagt actccgtgca cgttctcccg ggtacttacg
     2041 acttcgagac gacgggcaca tccgtaaccg gtctcgtcgt tccgccaggc acgaacacac
     2101 aaatcgatat cgatttgccg gaggcgccaa cgtactcggc gaccgtgcag ggctcggtga
     2161 agttcaatgg gcagccggtt ttgggtgggt gggggttcgt tttctacgag acggcgagcg
     2221 aagtcgaaag gtcgatagct ggcagtgggc caggctactc gttgagcatg ctccccggga
     2281 cgtacgacat cttctatgga gcgcagcgag acaactcgcc agagacgttg ctgctcccga
     2341 gaaacagccg ggctcctctg caacgtggcg tccgcgtgaa gtcgtcggcc acgaccaccc
     2401 tcgatctaga tgctccggtt ggcgttttga gaggcaagtt cacgattggt ggcgcgccgc
     2461 tacccgacgt atcgggcgat ggctcgctct gggtccgcgg cgcatcgggt gacgaagtcc
     2521 agctcgcttc gaactacgat ggcgactact cgtttccact catcgcgggc gactacgacg
     2581 tctacttcaa ggtggggctc ttcgggtccg tggcgccgcg gaacgctgcc gcgaacatcg
     2641 cgcgcatcaa cttgactcga ggcgacaacg ccatcgacat cgacgtaccg acgcgccaca
     2701 ttcagggcac cctgaccgtg agcggtgtga ccgtcggcgc gacgaacgac gtcgcaaaaa
     2761 tcaccctgca aggcccgaac ggcgacaccg taccgctcgg gagcgcctcc gccaaaacct
     2821 actcgaccac cgtggttcca ggcacctacg acatctactt cagcggcagc acgcggaaca
     2881 cgttcgcgcc gatgaaccag agcgccaaaa tcggttgtct gaaggtcccg taatcgaggc
     2941 ccagcacgcg agcctcactc cgtacaaggt gagcgctgcc ggcgcatcgg tcagtagagc
     3001 acgcgctgag tagcgcacga gccgggggac ccgtagcagg cgccgttgat gcagtatccg
     3061 ggaccgcagt cggggtcgag gctgcagtgc gtgcgctcac acgcgccggc gttgcacttg
     3121 gaaccactgg ggcaatctcc atcgagcgaa cagggtattt tatcgcagcg tcccgtccgg
     3181 tccgagcact tcaagaccgg gtcgacgcag tccgtagccg gagtgcattc gacgcagcgg
     3241 ttttgggcac aagtgccgcg cgagcaatcg ctcgtgtaca cgcagtccat gcagtggcgc
     3301 gccgagcccg ggccaggcga aacgcagaag gggtgctccg ccgggcaatc cgagttcgct
     3361 tggcactcgc tgcactcgct cagagtgata ccgggcgaat gaccaacgga gacgcaacga
     3421 ggtcgctcat tcggacattg agtgtctgat gtgcacgtcg cgctcgcatc cggcatcgcg
     3481 gcgcatccac actcgccgca ccattccttc gtaccgcagc cgacgaactc ggggagaggc
     3541 ggatcgaaag gagtcctgca aaaatagagt agcgaaccgg gcagcgttcc ggggtcgcag
     3601 tctttggcgg tggtgcagtg acccgcagag ttcgctccgg ccgcgcccgc cagagctgcg
     3661 ccggcggcgc cagcaacccc gacgatggtg gtgccgcccg ctccggctcc cgagcgccca
     3721 gcgcccacac cgccggatcc ggcggacatg cccccggttt gagctcccga gcgccccgct
     3781 gaagtcgacg cggccgtccc accgccgccg gttgcagagc tccccgcgat gccgcccgtc
     3841 gtgcccgcgc cgttccctga agcgttgccg ccgttccccg cgatggcggc accaccactg
     3901 ccggcagcct cgtccgggtc ggtcgtggtc gagcctcggc aggcagcgac cgacagcgcg
     3961 attgccgccc agagcgcggc ccgaatgcga ctcatgagcc ctccggagca atctccgtgc
     4021 cccgttttcg actcggctcg ctcacattag aaagcggcag ggtcgcgttg ctttgcccgg
     4081 aaattgcggg aaccgagggg attggagcga tcgcctcggt tgagccgtgc gtggcacaac
     4141 ttgcgccgac cgctgccaag cgcggccgtt tcggcgcccg gcggcgcatg gaatcgctcg
     4201 tctcttgcgc cttccgaagg catgctcggg gacctgaaac ggaccttaca agaatcgctg
     4261 ttcgagctcc cgccgcgcgt cgctcccgcg gagccgcgcg tcgagtacgc agaaatcgcg
     4321 gaacggctcc caccgacggt gcggcttggc acgatgtcgt gggcgttcga ggggtggcgc
     4381 ggcatcgtgt actcgagcac ctccgaggcg aagcggctct cgcacgaagg cttgcccgct
     4441 tactccgctc acccgctcct gcgcgccgtc gagatcgatc gctcgttcta cgagccccta
     4501 ccagccgccg cgttcacgca cttcgcctcg caagttcccc ctgactttcg cttcgtcgtg
     4561 aaggctcacg aagagaccac gctgcttcgc tttcctgatc acgctcgtta cgggaaaaag
     4621 cgcggaacat ccaattcgcg cttcctcgaa gcctcgtacg cgaccgacca agtcgttggt
     4681 ccggcgagcg aaggtttggg cgaaaagctc ggcactctgc tgtttcagtt cccgcctcag
     4741 tcgctcggtg aaccacgccg cttcgcggat gcgctcgagt ccttttttgc aggtctcccg
     4801 cgggggatcc cgtacacggt cgagattcgg aacgcggagc ttttgaccgc ggcctacggc
     4861 gcggctctcg cgcggagcgg cgtgatgcat tgccacaacg tttggggccc catgcccgac
     4921 gtgcgggagc aagcccggca actgccgaga gacgtgcgac agcgcctgat tgttcgttgg
     4981 ctgctccgcc ccggcgacac ctacgaaggc gcgcgttcgc gcttcttgcc gttcaatcgc
     5041 ctggtcgaag aggacggtac gacgcgcgcc tcgatcgtcg agctcgtggc gcgcgccgtt
     5101 tctttcgatg tgccgacgct cgtgctggtc aacaacaagg cggaaggcag cgcccccctg
     5161 agcctatttc gcatcgccga agcgctcgtc gcgcgcagcc ctcagaactg aattccgccc
     5221 gttccgaaca agctgaaggt gttgaagtcg gagacgatca agaagcgcgc gtcggcgccg
     5281 acgaacgctt tgccgatcgg atacatcacg gtcgcgccgg gccacacacc gaacttggtg
     5341 tcgctgctgg tttgctccgg gatcgtcacg ccgccgaagc tgccccctgg aatgctcgcg
     5401 gtcgcggtcg ccgcgccgag cccgacgtag ggccgaatca cgactggcgc cgcgtagatg
     5461 tcgtagccac cttcgacacc gaagtaatag agatgcacgg aggcgtcgcc gagcggtgtg
     5521 ctctcgctct tgccgaggtg gtaaacgaag gtaccgccga gatacacgtt ctgcggcaag
     5581 gtgtagccgc cgcgaacgcc gaggccgagg cccacaccgt ccttgaatcc gtagccgagg
     5641 agagcacccg cgctcgcgtg gcccgccgtc ggcgattctt cggcgtgagc ggcccccgtg
     5701 ccgagcagcg tgagtgagca agctgcgacc aaccccacta gtagtctctt catcgtaatt
     5761 ccattctcct ggcgatcgtg cctgagcagg aatacggcgg cgccgagcgc gtccttcccc
     5821 aaacgggtgg actcgcggcg caactcagct ggccggtggg gagccgaatg agcggcacta
     5881 cctcgacgcg gcggctcagc ccgaggtcat tagttcgacg aaagcgcgga tgcgcgcggc
     5941 ctttcgatgc tccgcgcgat gtacgagcca agtgccgatg ccgggggcgg cccagccggc
     6001 gaggagctga cgcaggcgtc cgctcgcgat gtcctcggcg accacccaat ctggcaggta
     6061 agcgacgccg agcccggcac gcacgagcgc tgcgatcgcc gccggtgtcg aggcgcgtac
     6121 cggcccccgc acgtcgactg cggtttcccg gccgtcgcga acgaagcgcc agggactgcc
     6181 gctgctcttg ccgagcgtgg tgaagcagac gtggtttgcg agctcgctcg gctccttcgg
     6241 aacgccgtgc tgtttgagat acgacggcgt tgccaccacc atgcggcgga tggtgagcat
     6301 ggggtgcgcg acgtaatcgg tgctgtccgg cggcgccaac ccggcgcgga tcgcgatgtc
     6361 gacgccgtgc gcgatgagat ccaccggctc gtcttccatg tgcaggtcga cgtggaggcc
     6421 ggggtgtcgc ttcaccagct gctcgagccg aggaacgacg tgctgcatcc cgaacgcgaa
     6481 cggaacgctg acgcgaatgc gaccggcgat cgaaggggcg gtgcgcgctg cggccatcgc
     6541 gtgttcggcc tcgcgcagcg cgacctttgc gtggtcgtag aaggtgcgcc cggcgtcggt
     6601 gaccgaaagc cttcgcgtcg acctcacgat gagggcggtg ccgagctgct tttcgagtgc
     6661 cgagagctgc cggctcacgg ccggaagtga caggcgggca gagcgcgccg cgccggacag
     6721 actgccggcc tccacgatgc gcacgaagat ctccatgtgt gagagcaggt ccacgcctca
     6781 gactactgca ccgccacgag atacgcgcaa tcatcgcgga cgatgattgt cgtgctggcg
     6841 caccccagac ccgagcgccc gcttcgcccc cgcgccattc gccgcgctag gacgcattct
     6901 ttacattttt gcaagagaca cttgcgcgcg gctcctctag ttgcaggccg ccgtgcgcct
     6961 agattctggt ccagccgagt tcacctcgag ctcgggagga gaacccatga atatccaagg
     7021 aaagaacgta ctcgtcaccg gcagcacccg cggaatcggt cgagccctcg tagaggcggc
     7081 cctggctcgg ggggcagcgc gcgtctacgc gaccgcccgc aaccgcgcgg cgctcgagtc
     7141 ttccgagctc cggcgcgacg cgcgcgtcgt cccgctcgag ctcgacataa cctcaccgga
     7201 gcaggcgcag cgcgcggcgc aactcgcgaa agacgtcgac gtgttgatca acaacgcggg
     7261 ggtactggct tcgttcgggg tgttgagcgg caccgctgcc gacgtgcaac gcgacttgga
     7321 caccaacttc ttcggcttgc tccatctggc gcgcgcgttc gcgccggtgc tcgccgagcg
     7381 caaggaggcg gcgctggtca gcgtcttgag cgtcgcttcg ctcgcgagta tgccggcgct
     7441 cggcggatac tcgagcagca aagccgctgc gtggtcgctc acccaggctc tgcgtgccga
     7501 gctcggcaag cgtggcgtaa agacattcgc ggtattcccc gggcccgtcg acacggacat
     7561 gattagaagc ttcgagctgc cgaagaccgc gcctgcgctc gtagcgcgcg caattctcga
     7621 cggcgtcgag gcatcggtgc tcgacatctt ccctgacccg atgtcgagag aagtgggcgc
     7681 tttgttcgcg aaggacccga tggcggtcga gcgtcagttc gcgaccccga tgtgagtcga
     7741 ctggttccca ctcgcggccc gggcttctgc caacgggccg cgcggggaag cgggcgaaac
     7801 ttgcctcgat ttcgatcgag gccactgccg tggcgaaacg agcctcgagc tcgagcgcag
     7861 ccagcgtcgc cggccaaacg ttgctcaacg gcgattcgcc ggcagggccg ccaaagcgta
     7921 gccgctcgcc gcgtcgacgc cccaatgcgc tgtcggcacg agctcgtcga gcgcactgta
     7981 ttgcgtgcga aagtggctac ccaaccagcg ctcgcaggtg agcgtgtcgt cggtgagcag
     8041 tcgatacttc ctcgagagcg tgatgcacgt ttcgaaggca gcgcgtgcgc gctgtctttc
     8101 gggctcccag ggatcgtcga aagtgccgtg gtagctcgcg ccgcggtcgt cgcaggcggg
     8161 accgcagagc tggtcgactc tcgccatttg gtccgacaga cgcccccaca gcgaaccgat
     8221 gcgcgccgcg actgccacgc gccactccgg cgcggccacc ggcggcgaca agtaaacctg
     8281 ttcgaagtga cgttgcgcaa tctcgtaggc ggcgtactgt cgatgggccc agggagcgaa
     8341 ctcgacggtg aggtagcgcc cgaaggcgcg gcgttgtcgt tcggcggagc cggcgacgcg
     8401 cacgaagacc ggcggcgcga gctcgagcgc tcgcagcctg agatccgcga cgtgaagcga
     8461 cgcgcgggcg gcggcggcgg cggctcgtcg aacgtctcgt tggctcgtct caccggttgg
     8521 caagccgcgg atccagtcga gcgcgagctc actgccccac agcctcgagg cgctctgcag
     8581 ctcacgttcg gcgcgtgccg cttctcccga accggcgagc gcatcgccgt agagtgaacg
     8641 tgctcgaacg cgaaggccga catcctggct ttcatcgatg caggggagct cgtcgaccag
     8701 caaagcggcc gcctccgcga atcgttcgct ctctacccaa gcgcgtgctc gctcgagtgt
     8761 tacaaagagc accgccaaac cggagatgtc gcaagccatt ctgaaaccat gcgacccgcg
     8821 agccgtgtcc ttcctttgcc gaagaagtcg ctttcgaacc cggtcgggag catcccgcgg
     8881 gccgaggtcg cgcatcgctc tcttgctcgc aatgctcgcg gtcgcctgcg gcactcctcc
     8941 caaatcgagg tctccggagc ccgagttgag cgacgtcgcg ccgctttcgg atttcgagcc
     9001 gacgcgctac cgaagcggtg ccccagtagc gagcccgatt gagccggcgg tgagtaccgc
     9061 gacggcggtt gcttgccgct tcgaagtgcc gatgtggagc gggccccttc aaatcactcg
     9121 cgcgaacgag ccgttcgcaa ccgtggccga cgcgcacgac gtccgcatcg ccgtgttcga
     9181 gacggatgtc gagcgcggcg catcagtcga gctcgatgtt ttgggcgtga ccctcgaggc
     9241 gctcgtgccg ttgtctgagc tcgagctgta cacgacgcgg gctcggcttt tcgacggctt
     9301 cgtgtgggct cttcccggcg catcgctgcg actcgagcgg gcgagtgctg ggcgcgtcgc
     9361 cgcgaagctc cgccttcccg ccgcgatcga gccagcgagt ccatcgtccc cgcgcgaact
     9421 cgcttgcgcc gagctaggag cggcgccggc taacgactcc ggccagctgc cgcgcgagtt
     9481 gttcggttcg cggcgggtcg ttcaatcggc gccgtttcgt ggtgatcgca gggtgccgct
     9541 gtcgtccaag ccgagcggcg agcccattgc ctttctcgac acgcgcccga gcgatccgga
     9601 gagtctgccc gacgaagcca tcgtgctcga acgccgtcgc gaccaggtgc gcatcgcata
     9661 tctgatggac gcgctcatcg tctttggatg ggtgccagcg cacgcgctgc aagcggaact
     9721 cgtagagacg ccgatcgagc tcgttcaggg actagcgccg tggggtcggc cggtggagcc
     9781 gtttggcggc gatgatccca cgcgcgtcga gctccccggc gagccacgca cgtgcgcatg
     9841 gaatgctccg ctcgcgatcg aagtcgcggg agcggtgcgc accgtgggca cgctcgccag
     9901 cgcgattccg ctgttgcctc tcgccacacg cgacggctgg cgtgaagtgg agttctcgca
     9961 tcccgcgctg tctttctcga agcgcgcccg cgcctgggtc cctgagcggc gcttgtatcc
    10021 ctgcacgccg cggcattgat tgatcgccgc tacggacggg caggcgctga tgatacgatg
    10081 gcccagaaga tgcttacgtg cgcgcccatg aaggcaagag ctctggtgct tctctgcgga
    10141 tgcgtggcgg tatcttgcct cgccacgccg cgcccgcagg atgctccggc cccgcgcgac
    10201 tattttcatc aggccaaggc gccgagctcg acgtgtgagc ctcgggtcga ggtgctgcgc
    10261 tccgcggcag aagcgcttcg gccttaccgt gaggtggcga cgctctcggc gagctgttat
    10321 ccaggcacgc cgcgcctctg cgaagaccgg ctcgcgcaac gcgcttgcga gctcaaggcc
    10381 gacgccgtca ttctcatgga accgacgtct ggcggtacac cgcccggcag ctctggtcag
    10441 tccgagatct cgatgagcgg tcgcgccgtg cgctggaatt cagagtaggc atcggcgcgc
    10501 gctggcgacg ggatgaactg ctactccgcg atgccgatcg tgctcgtcac ttggtgaggc
    10561 gcacgaagag cttgccgtgg tcgacgaaca cgacgtccgc ttttttgtcg ccgctcagct
    10621 cggccgcgaa gatctgattc gggtacgcgc cgacgctgta cgtgttcaag tttgggaact
    10681 tgccgccgcc tgcgttgagt cgaaccccaa agttgccact cgtatttctg ccgccggggt
    10741 ttgcaaacag caagtcgagc tttccgtcgc cgtcgagatc gcccgttgcg gccgcaatcg
    10801 gccccgtccc tgcgactccc gccgtagttg gcacgtcgag catcgcgccg ctgtcgtaag
    10861 tttgggcgct cgtcagcgtg ccatctccgc ggttgagcag aacgctcgca aatcctggcc
    10921 cgctatcctt gcccggggtg tacgtcagga cgtcggcgcg tgcatcgccg tccagatcgg
    10981 caaggagcac gctcgcaact ccagagggcg tcgggttcga atacgagacg ggtgcggcga
    11041 acactcccga gggactcgtg atgaacgcct tcagtgcctc gggatgcacg cgggtcacga
    11101 cgtcggcgct gccgtcgccc gtcaagtcac cggccgcgac gccgtcgacc tcggcgcggt
    11161 cgcaaatcgg ctgtggctct gcgaacgcgc ccgcgccgac gttcagcgcg aacgcgatct
    11221 tggtatcgcg aacgaagatt agatcgagct tgtgatctcc gttcaagtcg gcgctcagga
    11281 tgaagctgtt cgtggtcgtg accgtaaggt cgacagacgc tttcagctta ccgttgccca
    11341 ggttttgtag gaagtgaagc gcgccgccgt tcgagttgct ggtgatgaca tcgactgcgc
    11401 catcgccgtt gaaatcaccc gctatgaacg ctcccaaagc agtcgtttgc gcgtagctgt
    11461 cgcctgcctc gaaggtgcct ttccctttgt tgtggagaac gttcagtgac caatcggtgc
    11521 tggagcctgc gataacttgg gttgcaacga ttaggtcgcg cgtgccatcg ccgtccaaat
    11581 cagcgatttg aaccgacacc gcgtctttgg ggagcggata gttgaccgcg agtgcgaaga
    11641 agtcgttcgc gtccgtcgtg ccattgccac cgccgatgcc gcccgtgcct ccgccgtgga
    11701 gcccaccggt gaccgcggac tcgccgcccg ggcccagcgt cttgcccccg tcgccggatg
    11761 gcacgccgcc ggtgccgccg ttcttcgcgc cgctgctcgc tccacttccg ccgctcggag
    11821 cagtgctgtc ggccgtgccg cccgttccgc ccttcgtcgt gctgccgttc gtcgtgccgc
    11881 cggcgccacc agcgatcccg ccggcgccag acgttgcgcc gccatggttc gaagcgagcc
    11941 ctcctgtgcc ggtgcctccc gccccggaat gcagaccacc gctcgccgaa gagctgcgcc
    12001 cgcccgtgat tgcgccgccc gttccgccgg tctcatgtcc gccgtcgctc gcgctgtttc
    12061 cgcatgccaa cgcccccgcc gctgccgcga gcacgaaccc caacgaactg aattgccgcc
    12121 cctgccgcat catgtgccgc gccttcagca agagccgtgc ccccggacca attcgcaaat
    12181 gagcccgatt aaagccagtt actgtgtgcc gctcctcccg cgggtcgtct cgattggcac
    12241 acattggctc actcggcgag cattactttc gcttgcgagg cctgctttgg gcgcgccacc
    12301 gcgatcgagc ccaccccgtc gataagcgtg ctgctagggg gtgtcccaag ttcctgcccg
    12361 tcgcgagagc caagaaacgc gagcgag
//
LOCUS       DASTKS010000488         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1639422, whole genome shotgun sequence.
ACCESSION   DASTKS010000488 DASTKS010000000
VERSION     DASTKS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639422"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VFQ35_10145"
     CDS             complement(<1..1584)
                     /locus_tag="VFQ35_10145"
                     /inference="COORDINATES: protein motif:HMM:NF005901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1355c family protein"
                     /protein_id="HET9931040.1"
                     /translation="MKTINRLAAACDVKRPDLSSYKPALFRGDDHAELSRLAELVEGP
                     QVTAVHDALASQLTELAETRSPERKLSREELEAFAQRELAGRPADEYGVWVYYPWSRR
                     VVHVLPESEYRELRTSRNRNKITAEEQTVLRGLRLGIVGLSVGQATAVTLALEEIGGT
                     YLLADFDRLELSNMNRLRAGVHSIGVNKALLTAREIYEINPYARVEVFSDGIDESNIE
                     TFLVGERKLDILFEECDDLEMKIRLRERAREYGIPVLMETSDRGMIDVERFDLDRSRP
                     LLHGLVGDVRANSLKGLTTYEKVPIVLRIIGAETMSGRLAASLLDIETTLKTWPQLAS
                     NVTLGGALNADVARRVALGQFTGSGRYYVDLESAINDSVDGSVRVEAEADAVSGASPE
                     DVALPQLRSFAGELTETQVRDLVKYAISAPSGGNCQPWRFVYRRERFDCYFDRERSKT
                     YLDFDNTASYLAIGAAVENLCLAARQLGLDTELDTFPSPEHPDLVCSVRLTRASTNRP
                     AHDDLAAHIGARVTNRKLGP"
     gene            complement(2179..3183)
                     /locus_tag="VFQ35_10150"
     CDS             complement(2179..3183)
                     /locus_tag="VFQ35_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931041.1"
                     /translation="MSALRGAAERPLLPQVASAIVEIRRHLPLAAPSPTFRHLAFGSG
                     FLVQLRDASVRLLKSADGSAIAEVPLARPRVAVALPAGSVLACGSDECQRFDPGQVKP
                     HRLGRISLLPDAILEPSRETQERVWVIEPTFARAVRYTLGEPTSEVESTSELPGYDRG
                     AITTLADGAFLYTAGQSVVRALGRARRAMPLPDGVARPWRLLPAERADRAWVISAQGE
                     AFLLELTGRAHVLRRFATEGHPFDVASAKAALALVSIHEDQAEERTFVLNLYSAAGTQ
                     TKSYLLGKVGESADPEWAAMASRDREVVLAERPPRVAVGGSTALRLFELVSGDEIVTR
                     "
     gene            3350..3763
                     /locus_tag="VFQ35_10155"
     CDS             3350..3763
                     /locus_tag="VFQ35_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931042.1"
                     /translation="MSPPRLASVALLLVCTGCTRLHKTRECKDFAKLVNRRLDTIEAS
                     LAVKSAVNYRASSLTYAELSKEVRAAAKPLHPELAAEEFAQVFDSAAHATKNYALALD
                     SKDTQQQSELQRELERLARQEHSLVLRVNSHCENP"
BASE COUNT          778 a         1291 c         1238 g          692 t
ORIGIN      
        1 cggccccaac ttccgattcg tcacgcgggc gccgatgtgg gcagccaggt cgtcgtgagc
       61 aggccgattg gtggaagcgc gagtcagccg caccgaacat acgaggtcgg ggtgttcagg
      121 gctcggaaat gtgtcgagct ctgtgtcgag gcccagttgc ctcgcagcga gacacaagtt
      181 ttcgaccgcc gccccaatcg ccaagtaact agcggtgttg tcgaaatcca aataggtttt
      241 cgaacgttca cggtcgaagt agcagtcgaa ccgctctcgg cgatagacga accgccaggg
      301 ctgacagttg ccgccggatg gcgcgctgat ggcatatttc accaagtctc gcacttgcgt
      361 ctcggtgagc tctcctgcaa acgaacgcaa ctggggaagc gcgacgtcct ccggactcgc
      421 accgctgacc gcatccgctt ccgcttcgac cctgacgctg ccatccaccg agtcgttgat
      481 ggcgctctcg aggtcgacgt agtagcggcc cgagcccgta aactgtccca aagcgacgcg
      541 tcgcgcgacg tccgcgttca gggcaccgcc gagagtgacg ttcgaagcca gttgaggcca
      601 cgtcttgaga gtggtttcga tgtcgagcaa agacgcggcg agtcgtcccg acatcgtctc
      661 cgcgccgatg atacggagca cgatgggcac cttttcgtac gtcgtgaggc ccttcagtga
      721 gttcgctcgc acgtctccga ccaagccgtg caagagcggg cgggagcggt cgagatcgaa
      781 gcgctcgacg tcgatcatcc cgcggtcgct cgtctccatg agaacgggga tcccgtactc
      841 gcgagcccgc tcgcgcaagc ggatcttcat ctcgagatcg tcacactctt cgaagagaat
      901 atccaacttc cgttcgccga ccaggaaggt ctcgatattc gattcgtcga tgccatcgga
      961 gaaaacctcg acccgcgcgt acggattgat ctcgtaaatt tcgcgcgccg tgagcagcgc
     1021 cttgttcacc ccgatcgagt ggacgcccgc gcgaaggcga ttcatgttcg agagctccag
     1081 ccggtcgaaa tcggcgagca agtaagtgcc cccgatctct tcgagggcca gagtcaccgc
     1141 ggtagcctgc ccaaccgaca acccgacgat cccgagccgc aacccgcgaa gcactgtttg
     1201 ctcttcagcg gtgatcttgt tgcgattgcg gctcgtgcga agctcgcgat actcgctttc
     1261 tggcaataca tgcacgaccc gtcgagacca cgggtagtaa acccaaaccc catattcgtc
     1321 agcgggccgg ccggcgagct ctctttgtgc gaacgcctcc aactcctcgc gagagagctt
     1381 ccgctccggc gaacgcgtct ccgcgagctc ggtgagctga gatgcgagcg catcgtgaac
     1441 cgcagtgact tgcggcccct cgacgagttc ggccaaacgc gacagctcgg cgtggtcatc
     1501 gccgcggaac aacgcgggct tgtagctcga gaggtccggg cgcttcacat cgcacgcggc
     1561 cgctaggcga ttgatcgttt tcataccctt aatccttctt gatgtcgccc ctgccaagag
     1621 cgggtcgctc gtcgagggac gccggagaaa cgacgatcac gcgggacatg ccctgacgcg
     1681 caccgcacgt gtcctcatca ccccgggatg tgcaacgtcg gggatgagaa ccgccacgag
     1741 gcgcggatag gaggagattg cgccgccgca tgggagcgct gcgtccggat tctccgaaaa
     1801 atgcttgtaa gaagaccgac ctatcactcg gaacggaatc ggttggttcg ctttacccaa
     1861 gacggtgctg cgcgcgcgca gttgcgccga gcccaggcag aatttgcggt cgcgaaagtc
     1921 ctagacctcc tcttcgtgaa ctcgccaccc agcgagtact tctaagtatc gccgaaagcg
     1981 caaaaaaggt cagctctctt cgtacgcgcc cttcgacaat ttgtgttcga cttctacggc
     2041 acacccttca gggtaagagc cgaattcgcg cggtgggagg gtgtgcgatt ccggagaccc
     2101 acgtaccagc gcggcgccca gcgcgaccca cggaaggcag gcagccttac tcgctcgaac
     2161 gaggtttctc gtcgaccttc agcgggtgac gatttcgtca ccggagacga gctcgaagag
     2221 gcgaagcgcg gtgcttccac ccaccgcgac gcggggagga cgctcggcaa gaaccacctc
     2281 gcggtcccga gacgccatcg ctgcccactc ggggtccgca ctttcgccca cctttccgag
     2341 caagtaggac ttagtttgcg ttccggccgc gctgtacaga ttgaggacga aagtcctctc
     2401 ttcggcctgg tcctcgtgga tactgacgag cgctagcgcc gccttcgccg acgcgacgtc
     2461 gaaggggtgc ccctccgttg cgaatcgccg gagaacgtgt gcccgccccg tgagctccaa
     2521 cagaaacgcc tccccttggg cgctgatcac ccaggcgcgg tctgcacgct cagccggaag
     2581 cagccgccac ggacgagcga ctccatccgg taagggcatg gctcgtcgcg ccctgccgag
     2641 cgcgcgcacc accgactgac ccgctgtgta taagaaagcg ccatcagcca aggtggtgat
     2701 ggcgccgcgg tcgtaaccag gaagctcgct cgtcgattcg acttcggagg tcggttcgcc
     2761 gagtgtgtac cgaaccgcac gagcgaacgt tggctcgatg acccaaacgc gctcctgggt
     2821 ctctcggctc ggctcgagaa ttgcgtcggg aagcagcgag atgcgaccga ggcgatgcgg
     2881 ttttacctgc ccaggatcga agcgctgaca ctcgtccgac ccgcaggcga ggacacttcc
     2941 agctggtagc gctacggcga cgcgcggccg agcgagcgga acctccgcga tggcgctgcc
     3001 gtcagcgctt ttgaggagcc gaacgctcgc atcgcgcaac tggacgagga acccactccc
     3061 gaacgccagg tgacgaaatg taggtgacgg tgcagccaat ggcaggtgac gccgaatctc
     3121 tacgatggcg ctggcgactt gagggaggag cggtcgctcg gctgcgccac ggagcgctga
     3181 caccgccgat ggtgccaccc ccgcggaagc ggccggctcc cctacggtag ggcggttctt
     3241 ccccggcgtc gtcggcgtcg gtcgctccgt tggtagagga ggacctttct cgcaccccca
     3301 gagccccgtg ggcagcagcg cgcaccataa gatgctaagc agggcggccg tgagcccgcc
     3361 tcgcctcgcc tcggtagccc tgttgctcgt ctgcaccggc tgtactcgcc tacacaagac
     3421 ccgcgagtgc aaggatttcg cgaagctcgt caatagacgg ctcgacacca tcgaagcgtc
     3481 gttggcagtg aagtcggcgg tgaactaccg agcatcgtcg ctcacgtatg cagagctctc
     3541 gaaggaggtc cgcgccgcag ccaagcccct tcaccctgag ctcgcagccg aggagttcgc
     3601 gcaggtcttc gactcggccg cgcacgctac gaagaactac gctctcgcgc tagacagcaa
     3661 ggacactcag cagcaaagcg agctccagcg agagctcgag cgactcgcac ggcaagaaca
     3721 tagtctcgtg ctgcgggtca actcacactg cgaaaaccct tagcagggtg ttgaaaaacg
     3781 gcagcggctc gggctcgtcg ttgcgctcct cgacctgctt cagccacagg attcgtcaca
     3841 tgtcgcgaac aggtaggcct tcctgcccgt cgcgagagcc aagaaacgcg agcgagaagc
     3901 gcgcccgagg agtgagcacc ggaggaaggc tgaagccttt cgaggagcgg agcgacgagg
     3961 ccgtgcttcg cagccgcagg atcttggctc gcagcaggg
//
LOCUS       DASTKS010000489         5724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1639904, whole genome shotgun sequence.
ACCESSION   DASTKS010000489 DASTKS010000000
VERSION     DASTKS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639904"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..806
                     /locus_tag="VFQ35_10160"
     CDS             300..806
                     /locus_tag="VFQ35_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9931043.1"
                     /translation="MQSSQEVEFKVGDKAVYPAQGVAEVISIDEKDIAGQRQRFYVLR
                     ILDTDRKIMVPVSNANAVGLRQVISEQEIREIFDILKERTIGFDTQTWNRRYRGFMDK
                     IKTGSIYDVAEVLRDLYRLKANKQLSFGERRMLDTARGLIVKEIAIARAQTEDDVKNE
                     IEAIFFSN"
     gene            complement(1011..1547)
                     /locus_tag="VFQ35_10165"
     CDS             complement(1011..1547)
                     /locus_tag="VFQ35_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931044.1"
                     /translation="MKFPSARHAVASPFVKSQGARHAVASPNAVALVFTFALTLGACE
                     REPVDARPEQVVQEFAQRMQRVHGDDPKSARAAYELLWVEARRNLAERAKRASAVAGR
                     KIAPEEMLAPSRFSLKFVPKRYTSTIDGDWALVAVSGGGPDGPTHNVRCVREDGHWRV
                     VLELPPLAPIQHRADGGV"
     gene            1566..2651
                     /locus_tag="VFQ35_10170"
     CDS             1566..2651
                     /locus_tag="VFQ35_10170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9931045.1"
                     /translation="MPELGQAAAALVTISNTMSIPEHGVRLEWSGKSAEKPELSASLE
                     LIRQGNAATRLVRGDNLHALSALAKRPERAMLVYLDPPFFTGREHNHVERSRGDEGIV
                     RKLVPAFDDRWESLEHYLGELAKRLRLARDVLDPAGCLVLHVDPKTSHYAKVLCDEIF
                     GLDAFASEIVWRYRRWPAKTKNFQRVHDVLLRYVRDPSQEPRFQQLYEPLAASTEKTW
                     GTRKQRAVVDSSGRRTRSSVTEQASPGAPLGDVWDISIIAPVARERTGYPTQKPEALL
                     ERLIQACTAPSDIVLDPYCGSGTTLAVASRLGRRGIGIDQSEQALAITYKRLESLGVA
                     PQQEAVRLEAKPSLLKTQGRSRAQQVA"
     gene            2790..3776
                     /locus_tag="VFQ35_10175"
     CDS             2790..3776
                     /locus_tag="VFQ35_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9931046.1"
                     /translation="MRSRAIFFACCLASAAAAAANADATAPVVRVGELSATGADITRR
                     LSTLSAGQFAAYGGGAEVPRRYVDEVVVPELAATLEARQRGVDKSPAYRDRERVALRQ
                     ALEAALKADAIAKKPVTQEDIKAYFEANKARFEQPLAIRLWRILVDDEASAKRIIEQV
                     KDAGSPAKWSELARDSSVDKATNLRQGDLGFVHADGGTDVPQVRVNPALFQAAKDLKD
                     GAVVPTPVHEGAKFAVVWRRGSRPERTRTLEQERDSIRSLLERQRAEDARSSLLTELR
                     QKYLREEHPELLDEIPEGMFGSKLARPRPMLVPPHARSIGTKLPEPTPQGLR"
     gene            complement(4056..5429)
                     /locus_tag="VFQ35_10180"
     CDS             complement(4056..5429)
                     /locus_tag="VFQ35_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063796744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HET9931047.1"
                     /translation="MRQMVHDQLRLTPVPIAHVHARELQAMSATLDALPSVVKWVHDD
                     LIRRAGNRIDPKKGRQGMTAEQVLRALIVKQMNGFSYEQLAFHLADSTSYRAFCRLGF
                     DDPSPKKSRLQSNIKRVRAPTWERINHELVRYAAALRVEMGLTVRTDCTVVESNIHQP
                     TDSSLLWDCVRVLVRLQGRAQEMFGINFTNHKRRARRRALGILNAKSDVQRAPLYRDL
                     LKATEASVASAKNVVAALGELKFADATTMLHAATIADQLRIYCKLARRVIDQTRRRVL
                     LGEQVPAGEKVVSIFEPHTDIIVKDRRATLYGHKICLTSGASGLVTDVVVEEGNPADS
                     TLAVKMVERHRERFGKAPRQVSFDGGFASRTNLAELKAMGVQDVAFSKHLGLSVTDMV
                     KSSWVYKRLRNFRAGIEGTISFVKRIFGLDRCLWSGFASFRAYVQGSVLACNLLVLAR
                     HRLASSA"
BASE COUNT         1064 a         1814 c         1857 g          989 t
ORIGIN      
        1 caccgtaggt tgacagatcg gaagaacccg actagagtgc cgcgcctggc gaaacctgac
       61 tcaaattcag gcgggccagc gctcgggttt ccggaaacca cggcgaaatg ccgacggaac
      121 ccgctgcgtg agaggctgga atgaaactga agtcccgttc gttgttgtcg cgcccagtac
      181 tgaggtcgcg aaaaggaaac gggagctagc cgatttatcg ggttgatggt cgctcgctcg
      241 cgctgtgccg ggtcaatgcc ggtccgcgat cggagtgatg tgcagttcaa ggagacgtca
      301 tgcaatcgag ccaggaagtt gaattcaagg tcggtgacaa ggcggtctac cccgcgcagg
      361 gagtagccga ggtgatcagc atcgacgaga aggacatcgc cgggcagcgc cagcgtttct
      421 acgtcctgcg gatcctggat accgaccgca agatcatggt tcctgtgtcc aatgccaatg
      481 ccgtcggcct gcgtcaggtc atcagcgagc aagagattcg cgagattttc gacatcttga
      541 aagagcgtac gatcggcttc gatacgcaga cttggaaccg ccgctatcgc ggcttcatgg
      601 acaagatcaa gactggatcg atttacgacg tggccgaggt gctgcgtgat ttgtatcgcc
      661 tgaaggcgaa caagcagctg tcgttcggcg agcgccgcat gctggacacc gctcgcggtc
      721 tcatcgtgaa ggaaatcgcg atcgcccgcg cgcagacgga agacgacgtc aagaacgaga
      781 tcgaagcgat cttcttctcg aactgagttt tctagtcatt cgtcgaaggc gcacaccaaa
      841 gcggtgtgcg ccttttgcgc tttgtggaga cgacctgcgg gagtagcgca ggcgctgagc
      901 gcgcttcaga cgccgccgtc ggcgcggtgc tggattcacg cgagtgcggt actcgagcgc
      961 gccgcgcctg gtcacgacgg cttccgcaag cgggcaggcg cgagcgcgcc tcagacgccg
     1021 ccgtcggcgc ggtgctggat cggcgcgagt gggggtagct cgagcaccac gcgccagtga
     1081 ccgtcttcgc gcacacagcg gacgttgtgc gtcggaccgt cgggcccgcc gccgctcacc
     1141 gcgacgagcg cccagtcgcc gtcgatggtc gacgtgtagc gcttgggaac gaacttgaga
     1201 gaaaagcgcg acggcgcgag catctcttct ggagcgatct tgcgaccggc aaccgcactc
     1261 gcgcgcttgg cccgctcggc gaggtttcgg cgcgcctcga cccacaagag ctcgtaggcc
     1321 gcgcgcgcgc tcttcggatc gtcgccatgc acacgttgca tgcgctgggc aaactcttgc
     1381 accacctgct ccggtcgcgc gtccaccggc tcgcgctcgc aggcgccgag ggtgagcgcg
     1441 aacgtgaaca cgagcgcaac cgcattgggt gatgcgacgg cgtggcgagc gccctgagac
     1501 ttcacgaagg gtgatgcgac ggcgtggcga gcgcttggga acttcacgaa gtgcacggcc
     1561 cgagcatgcc agaacttggc caagcggcag ccgccctcgt tacgatttcg aacacgatgt
     1621 cgatccccga gcacggcgtg cgattggagt ggtccggtaa gagcgcggag aagcccgaac
     1681 tgagcgcctc actcgagctc atacgccagg gaaacgccgc gacgaggctc gtgcgcggcg
     1741 acaacttgca cgcgctctcc gcgctcgcga agcgcccaga gcgagcgatg ctggtctacc
     1801 tcgacccccc attcttcacg gggcgcgaac acaatcacgt ggagcgttca cgcggtgacg
     1861 aaggcatcgt tcgcaagctc gtgcccgcat tcgatgaccg ctgggaaagc ctcgagcact
     1921 acttgggcga actcgcgaag cggcttcggc tggctcgcga cgtgctcgac cccgctggct
     1981 gcctggtgct ccacgtggac cccaagacga gccactacgc gaaggtgctc tgcgacgaga
     2041 ttttcgggct cgacgccttc gcgagcgaga tcgtttggcg ctatcggcgt tggccggcca
     2101 agaccaagaa ctttcagcga gtccacgacg tgctgttgcg ctacgtgcgc gacccgtctc
     2161 aagagccacg ctttcagcag ctgtacgagc cactcgctgc gtcaacggag aagacctggg
     2221 gcacgcgaaa gcagcgcgcg gtcgtcgact cgtcggggcg tcgcacgcgg tcgagcgtta
     2281 ccgagcaagc gtcgccgggt gccccgctgg gtgacgtctg ggacatttcg atcatcgcgc
     2341 ccgtcgcgcg cgagcgaacg ggttacccga cgcagaagcc cgaggcgctg ctggagcgct
     2401 tgatccaggc ctgcacggca ccttcggaca tcgtgctcga tccgtactgc ggtagcggga
     2461 cgaccctagc cgtcgcctcg cgcctcggac ggcgaggcat cggcatcgat cagagcgaac
     2521 aggcgctcgc gattacgtac aagcgactcg aatcgctggg cgtcgccccc cagcaagagg
     2581 cggtgcgcct cgaggccaag ccgagcctgt tgaagacgca aggaaggtcg cgagcgcagc
     2641 aggttgcttg agacccgtcg cggagaaagt gcaccgcgca ttgcttccgg cacacggatg
     2701 cagggcgctc gttcgagacg cgggtcggca cgggccgcgc gttgcttgag ccgcttgttc
     2761 gccggagccc gccggcataa ggtcgcggcg tgcgctcgag agccatcttt ttcgcctgtt
     2821 gcctagcgtc tgcggctgcc gccgcggcca atgccgacgc gacggctccc gtcgttcgag
     2881 tcggtgagct cagcgcgacc ggagccgaca tcacgcgccg gctgtctacc ctgagcgcgg
     2941 ggcaattcgc cgcgtacggt ggcggcgccg aagttccgcg gcgctacgtc gacgaggtgg
     3001 tcgttccgga gcttgccgcg acgctcgagg cgcggcaacg cggagtcgac aagagcccgg
     3061 cgtatcgcga tcgcgagcgc gtcgcgctcc ggcaagcgtt ggaggcggcg ctcaaggcgg
     3121 acgccatcgc gaagaagccg gtcacccagg aagacatcaa agcctatttc gaggccaaca
     3181 aggcgcgatt cgagcagccg ctggccattc ggctctggcg catcttggtc gacgacgaag
     3241 cgagcgcgaa gcgcatcatc gagcaggtga aggacgccgg ttctcccgcg aagtggagcg
     3301 agctcgcgcg tgacagctcg gtcgacaagg ccacgaacct tcgtcaggga gacctcggtt
     3361 tcgtccacgc tgacggcggc accgacgtcc cccaggttcg cgtcaatcca gcgttgtttc
     3421 aggctgcgaa agatttgaag gacggcgccg tggtgccgac tcccgtccac gagggcgcca
     3481 aattcgcggt ggtgtggcgg cgcggttccc ggcccgagcg aacccgcacg ctggagcagg
     3541 agcgcgactc gattcgctcg ctgctcgagc gacaacgcgc cgaagacgct cgctcgtcgc
     3601 tgctcaccga gctgcggcag aagtacctac gcgaagaaca cccggagctc ctcgacgaaa
     3661 tccccgaggg catgttcgga agcaaactcg cgcggccccg gccgatgctc gttccaccgc
     3721 acgcgcggtc aatcggcacg aagctccctg agccgacgcc gcaaggccta cgctgaaagt
     3781 agcctcgaag aggctcggga ccggagcgac gagccgatcc gacgccgcaa gagggtcggc
     3841 tcgcgcaata tgctcacccg ttcgcgggcc ctagctagtt cccggccaga aatggccaag
     3901 tttgaaaact ggcagcgatc aaaccgaagc cggagaggta ccgagagagc gcgcggcacg
     3961 gaatcggtgt ccgacttcgg tgagggccac agaatccgtg gctgcggggc gcacgtatcc
     4021 cttggcccag cgtgttggcg agagcgattg gctgcctacg cggaggaggc gaggcgatgg
     4081 cgcgcgagca ccagcaggtt gcaggccagc acggagcctt gcacgtacgc ccggaaggaa
     4141 gcgaagccac tccagagaca gcgatcgagg ccgaagatgc gcttgacgaa cgagatcgtt
     4201 ccttcgatgc cagcgcgaaa gttgcgcaac ctcttgtaga cccagctgct cttgaccatg
     4261 tcggtcaccg agagaccgag gtgtttgctg aacgccacat cctgaactcc catggctttc
     4321 aactcggcga ggttggtccg cgacgcgaat ccgccgtcga agcttacctg ccggggtgcc
     4381 ttgccgaagc gctctcggtg ccgttcgacc atcttgaccg ccaaggtcga gtcggccggg
     4441 ttgccctctt cgacgacgac atcggtgacg agaccggacg cgccggaggt caagcagatc
     4501 ttgtgaccat agagcgtcgc tcgacggtcc ttcacgatga tgtcggtgtg cggttcgaag
     4561 atcgaaacga ctttctcgcc agcggggact tgctcgccga gcaacacgcg ccgacgtgtc
     4621 tgatcgatca cccggcgagc caatttgcag tagattctca gctgatctgc gatcgtggcc
     4681 gcatgcaaca tcgtcgtcgc atccgcaaac ttcagttcac cgagcgctgc tacgacattc
     4741 ttggcactcg ccacggaagc ttcagtcgcc ttcagcaagt cgcgatacaa gggcgctcgc
     4801 tgcacgtccg acttggcgtt gaggatgcca agcgctcgtc gtcgcgcacg tcgcttgtga
     4861 ttggtgaagt tgatcccaaa catctcctgg gcgcgccctt gcaggcgcac cagcacacgg
     4921 acgcaatccc agagcaacga cgagtccgtc ggctggtgaa tgttggactc gaccaccgta
     4981 cagtccgtgc gaactgtcag gcccatctcg acacgcagcg cggcggcgta gcgtaccagc
     5041 tcgtggttga tgcgctccca cgtcggcgct cgcacccgct tgatgttgct ttgcagtcgc
     5101 gacttcttcg gcgaggggtc gtcgaacccg agccggcaaa acgcgcgata gctggtcgaa
     5161 tccgcaaggt gaaacgccag ctgctcgtag ctgaagccgt tcatttgctt gacgatcagc
     5221 gcgcgcagca cctgctcggc ggtcatccct tgacgccctt tcttaggatc gatccggttg
     5281 cccgcgcggc ggatcaggtc gtcatgaacc cacttcacga ccgagggcaa ggcgtccagc
     5341 gtggcgctca tcgcctgcag ctcccgagcg tgaacgtgcg cgatcggcac tggcgtaagg
     5401 cgaagctgat cgtgtaccat ctggcgcatc ggagtgtttc tcctttgaga gcattggcgt
     5461 gcttctccga ggaacaggca ctccgagcca attatttcca tcgcccgaag atcggttttc
     5521 tcttttgttt tcgcgcgatt gccatttctg gccgggaact agctagcgac tctctccgcg
     5581 ttcacgcaat attcgcgcgg cttctctata caccgcgcgt tgcggcattg cctcggccaa
     5641 acgctcgtag acctgcgcgg cgtccgcgaa tcgcccttcg acgacggcgt cactcgcagc
     5701 gcgctccaag ctgatgcccg tttc
//
LOCUS       DASTKS010000490         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1194094, whole genome shotgun sequence.
ACCESSION   DASTKS010000490 DASTKS010000000
VERSION     DASTKS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194094"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..538
                     /locus_tag="VFQ35_10185"
     CDS             38..538
                     /locus_tag="VFQ35_10185"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9931048.1"
                     /translation="MSTFLLKLADMRFPVRIGETLLGRSPYCTIVLDDPRVSRQHAAI
                     RMTAEGLSIQDLGSRNGTLVNGQKLRGSLVLQAGDVVQLGAQEIEIELGADAATSSRR
                     RLADTGDFTAAAASHFEGTGDAPDAHSETETPTEPGTPPNSLRAAHARKLSPPAGTST
                     GRGVVT"
     gene            complement(895..1452)
                     /locus_tag="VFQ35_10190"
     CDS             complement(895..1452)
                     /locus_tag="VFQ35_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931049.1"
                     /translation="MFAEKVQIKLFASVDTHTEAYVPVFHRWIRENVLNELMIDVVDY
                     THVASGPEVLLLGHASDYVLDRGDGKLGLLYAAKREPLTEEGSFLPALRRALRAARLL
                     SAETAVKQPLAFKSDSLLVRIADRLNSPNDDATLARVEPALRSALASIYGDISARITR
                     VGDPRDLFSVEVSIPGAPALDKLLA"
     gene            complement(1491..3740)
                     /locus_tag="VFQ35_10195"
     CDS             complement(1491..3740)
                     /locus_tag="VFQ35_10195"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET9931050.1"
                     /translation="MTSSWKRVLEGKIREDWAREIDVFETQIELRKKGKLEEKLFAET
                     RLRRGVYGQRYDNGQRHDGEKTQTLGFPCGELTKGPVTVWDAPGMMRIKIPMGRLSAQ
                     QLELQCELAEEYSDQILHVTTRQDIQLHFVHIEDTPDLMRRLASVGITTREACGNSVR
                     NVTACPYAGVCNEESFDVAPYAHAAAFFLLGHDDTQDMGRKFKIAFSGCHDNPCGLTN
                     FHDLGCIARTRKVDGVVQRGFEFYVGGGLGAVPQSALLLDEFLPEAELLPMAQAVCRV
                     FARLGEKENRQRARLKFLVKKLGLEEFKRLVLEERTKLRPDPRWSSFLNDLGHTDEKP
                     LRAPSELPKGPFAPAFERWLKTNVEPQRQAGYSTAKVTLPLGDFTPSQGRKIADLMRR
                     FTGDTLRLTVEQNLLFRWVSNGDLPALYAELEAIGLGDAGADTITDVTSCPGTDTCKL
                     GISSSRGLAGELRRRLKLVEETLDPAARELHIKCSGCFNSCGQHHVADLGFLGVSRNV
                     GGRRVPHFQLVVGGELAGNARSYGLAIGAVPSKRVPEVVERLTREYVTGKKADETFAQ
                     FIERFGKKEVRKIVDQFNTVPSYEEDPSFYSDWGDPREYTIGDLGVGECAGEVVSFTQ
                     FGLAASEREVFEAQVLLDEGKIREAADRAYSAMLQAARALTRQQNPNLGEDPGEIVNE
                     FKARLVDTKVFWDPFAGAKFAQYLFRAHADPAIKGQNQESAHQLIEEAQLFVDAAHQA
                     YTRIGNVVP"
BASE COUNT          665 a         1224 c         1225 g          668 t
ORIGIN      
        1 tcaagggttg cgctgactcg ccgtagactc ttttcggatg agcacgttct tgctcaagct
       61 tgccgacatg cggtttcccg tacgcatcgg ggagacgctg ctcggtcgta gcccgtactg
      121 caccatcgtc ctcgatgatc cgcgcgtttc gcggcagcac gcggcgattc gcatgacggc
      181 cgagggactc tcgattcaag acctcggcag tcggaacggc acgctggtga acgggcagaa
      241 gctgcgtggg tcgctcgtgc tgcaagccgg agacgtggtt cagctcggcg cgcaagaaat
      301 cgaaatcgag ctcggtgcgg acgctgcgac ctcgagccga cgccgtcttg cggatacggg
      361 cgactttacg gcggcagcgg cctcgcactt cgagggcacc ggcgatgcgc ccgacgcgca
      421 cagcgaaacg gagacgccta ccgaacccgg cactcctccg aacagtctgc gtgccgcgca
      481 tgcgcgcaag ctgagccctc ctgccgggac cagcaccggc aggggcgtcg tcacttagag
      541 ccactcccaa atcctggacc gagcccggca aagagacgcg agcagtccga ggcgagacga
      601 cgaggcgtac tgaagtctca ggatttatcg gatctccgaa ccacgcggcg gcccgccgtt
      661 ttcggggttc ccggggcccc gaaaaccaca caaaccaaag catcggaacg aactctttcg
      721 aagcacgggt cggaaccaac tctttcgtgc cgagccctct gcgagcgaac gagacgaaag
      781 cctacctgct ggcgacatgt gacgaatcct gagactgaag tacgccgagg aggagcggat
      841 ctgccgccgg gcgacgggag ggatctagga ttggctctta gtcgccttgc gccttcaggc
      901 gagaagcttg tcgagcgccg gagcgcccgg gatgctcact tcgaccgaga acaagtcgcg
      961 cgggtcgccc acgcgcgtga tacgggcgct gatgtcgccg taaatgctgg cgagcgccga
     1021 gcgaagcgcc ggttcgaccc gggcgagcgt tgcgtcgtcg ttcggcgagt tcaagcggtc
     1081 ggcgatgcgc acgagcaggc tgtcggactt gaaggcgagc ggctgtttca ccgctgtctc
     1141 agcggagagt agtcgcgcgg cgcggagcgc gcgacgcaac gccggcaaga aagagccttc
     1201 ttcggtcagg ggctcgcgct tcgccgcgta cagaagaccg agcttgccgt cgcctcggtc
     1261 gagcacgtag tccgacgcgt gaccgagcag cagcacctcg ggaccgctcg cgacgtgcgt
     1321 gtaatcgacg acgtcgatca tcagctcgtt caggacgttc tcgcgaatcc agcggtgaaa
     1381 caccgggacg taagcctcgg tgtgcgtatc gacggacgcg aacagcttga tctggacctt
     1441 ctcggcgaac atcttcggta tctcgctagg cgaaagggaa aacgggacgc tcagggaacg
     1501 acgttcccga tgcgggtgta cgcctggtgc gcggcgtcga cgaacagctg cgcctcttcg
     1561 atcagctggt gcgcgctctc ttgattctgc cccttgatcg ccggatcggc gtgagcgcgg
     1621 aacaggtact gcgcgaactt ggcgccggcg aagggatccc agaacacctt cgtgtcgacg
     1681 aggcgcgcct tgaactcgtt gacgatctcg cccggatctt caccgaggtt cgggttttgc
     1741 tgccgcgtca aggcgcgcgc cgcttgcagc atcgcgctgt aggcgcgatc ggcggcctcg
     1801 cgaatcttgc cctcgtcgag gagcacttga gcctcgaaca cttcgcgctc gctcgcggcc
     1861 aagccgaact gagtgaagga tacgacctca ccagcgcact cgccgacccc gaggtcgccg
     1921 atggtgtact cgcgcgggtc accccagtcc gagtaaaagc tcggatcttc ctcgtaggaa
     1981 ggcaccgtgt tgaactggtc gacgatcttg cggacctcct tcttgccgaa gcgctcgatg
     2041 aactgagcga aggtctcgtc cgccttcttt ccggtcacgt actcgcgcgt caagcgctcc
     2101 acgacctccg gcactcgctt cgacggcacc gcgccgatcg cgaggccgta agaacgagcg
     2161 ttgccagcga gctcgccccc gaccaccagc tgaaagtgcg gtacgcggcg gccaccgacg
     2221 tttcggctca cgcccaagaa gccgagatcc gcgacgtgat gctgcccgca cgagttgaag
     2281 cagccgctgc acttgatgtg cagctcacgc gcggcgggat cgagcgtctc ttccacgagc
     2341 ttcaagcgcc gacgcagctc accggcaagc ccgcgtgaag acgagatgcc gagcttgcag
     2401 gtgtcggtgc cagggcacga agtgacgtcg gtgatcgtgt cggcgccagc gtctcccaag
     2461 ccgatcgctt cgagctcggc gtagagcgcg ggcaagtcgc cattcgatac ccacctgaac
     2521 aataggtttt gctcgacggt gaggcgcagc gtgtctcccg tgaagcgccg catgaggtcc
     2581 gcgatcttgc gcccctgcga gggcgtgaag tcgccgagcg ggagcgtcac tttcgcggtc
     2641 gagtagcccg cttgccgttg cggctcgacg ttcgtcttca gccagcgctc gaacgccggc
     2701 gcgaacggtc ccttcgggag ctccgaaggc gcgcgcagcg gcttttcgtc ggtgtgaccg
     2761 aggtcgttca ggaacgacga ccagcgcgga tccggccgca gcttcgtgcg ctcttcgagt
     2821 acgaggcgct tgaactcttc gagcccgagt ttcttcacca ggaacttcag gcgcgcgcgc
     2881 tgtcggttct ctttctcgcc caggcgagcg aatacgcggc acaccgcctg cgccatcggc
     2941 aagagctccg cctccggcaa gaactcgtcc aacagcaacg cgctctgcgg cacggcgccg
     3001 agcccaccgc ccacgtagaa ctcgaagccc cgctgcacta cgccgtcgac cttgcgcgtc
     3061 cgggcgatgc agccgaggtc gtggaagttg gtgaggccgc aggggttgtc gtggcagccc
     3121 gagaaggcga tcttgaattt gcggcccatg tcctgcgtgt cgtcgtgacc cagcaggaag
     3181 aacgccgccg cgtgagcgta gggagcgacg tcgaaactct cttcgttgca gacgccggcg
     3241 taggggcagg cggtcacgtt gcgcacggag ttaccgcacg cctcgcgcgt ggtgatcccg
     3301 accgacgcca gacgacgcat cagatccggt gtgtcctcga tgtgtacgaa gtggagctga
     3361 atgtcctggc gcgtggtgac gtggaggatc tggtccgagt actcctcggc gagctcgcac
     3421 tggagctcca gctgctgcgc cgagaggcgt cccatcggga tcttgatgcg catcatcccg
     3481 ggcgcgtccc acaccgtgac cggccccttg gtgagctcac cgcacgggaa cccgagggtc
     3541 tgcgtcttct ccccgtcgtg gcgctggccg ttgtcgtagc gctgaccgta gacgccgcga
     3601 cgcaggcgcg tctcggcgaa gagcttctct tcgagctttc ccttcttgcg aagctcgatc
     3661 tgcgtctcga aaacgtcgat ctcgcgcgcc caatcctcgc gaatcttgcc ttcgagcacg
     3721 cgcttccaac tagaggtcat gttttcttgc tccgcacggc cggaattaaa cggtctcggg
     3781 tt
//
LOCUS       DASTKS010000491         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_970597, whole genome shotgun sequence.
ACCESSION   DASTKS010000491 DASTKS010000000
VERSION     DASTKS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970597"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFQ35_10200"
     CDS             <1..367
                     /locus_tag="VFQ35_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9931051.1"
                     /translation="KQSEFDAREQSPDEPFWLTWRSYLQSLVDAGVYVGGSPLERPDT
                     TATTLRVRGGERQVQDGPYADTKEQLGGFMVLELPNLDAALDWAARCPAASYAAVEVR
                     PAANLERFFGPEPGSLFRG"
     gene            360..1589
                     /locus_tag="VFQ35_10205"
     CDS             360..1589
                     /locus_tag="VFQ35_10205"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HET9931052.1"
                     /translation="MGEAERAVESVVRESYGRLVSFLAARSGDVSSAEDALSEALVAA
                     LRSWPTAGIPTQPEAWLLHAARRRVIDQHRSRKLRDEHAESFRSVLEQAEEMANGFQF
                     PDERLKLLFVCAHPAIDERVHTPLMLQVVLGLDAAAIAQAFLTPAATMGQRLSRAKAK
                     IRDTRIAFQVPAGEELAPRLFAVLEAIYAAYNRGWDDVDGADEQRRGLTDEAMWLGRL
                     LVELMPNEPEALGLLALMHFCEARRPARRDSKGEYVPISAQEPRAWSSEHLGAAQLLL
                     ARAARLGRIGRFQLEAAVQAAHCERAQTGRTDWPAIALLYEGLVRLAPTVGALLGRAA
                     AAGEAHGAKAGLELLLQIPESVVAEHQPYWTVRAHLEQRLGRQAESFAHFERALELTE
                     EPAVRRFLERSIRIPQG"
     gene            1826..3004
                     /locus_tag="VFQ35_10210"
     CDS             1826..3004
                     /locus_tag="VFQ35_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931053.1"
                     /translation="MAISISAWACGSSQGDAGSGGTSNASKGGDTSSTGGQSAASGGT
                     TASVGSGGAATSGGSGNAPGSGGAPGSSGAAARGGSQGLGGSSNSGSGGRSVGRGGGS
                     SAGGASAGGSSAGSANGGAAQSGDNCTIGKWPSADPTMTGPYATVTENEVGPEAGAVD
                     ADTGMVPKFTLFRPKDLSPGGLCHPIITWGNGTGATPNLYRSLLGLFASHGFVVIASN
                     SKNVAQGDPRPMLVGVTWVLEQNEDPTSPLYHHLDPTHIGATGHSQGAVATSQASGDA
                     RITTNVPIEGAMVQRNLHGPAMFFCGGMDDVVGCDGAKSALNAVTTLPAMYAEYLSVD
                     HGSWMSYGNKPSVVYGAVTAWMRVHLMSDDSLRPWFYGESCKLCTDTGWQVQQKNMPQ
                     "
     gene            complement(3070..>3683)
                     /locus_tag="VFQ35_10215"
     CDS             complement(3070..>3683)
                     /locus_tag="VFQ35_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9931054.1"
                     /translation="LDHVLLDASPDVYLYSIGVDPNLASEVGRAATDAPTLPLRSQIR
                     PSELEHLIQRCSDVVDRAGVDDDCAELWQRAQWLQSNSTSLGGAVHVLTRRALAELTR
                     FPDVSRESLARTLRASPSDVSRYFHRDVGVTLVQYRTRARLLRLIREVDRGERNLKEL
                     ACQVGFGSYSQCHRAFMAELRCAPRAFFVSGMRQQMQDSYALK"
BASE COUNT          633 a         1147 c         1281 g          622 t
ORIGIN      
        1 aaaacagtcg gagttcgacg cgcgcgagca atccccggac gagccgtttt ggttgacctg
       61 gcggagctac cttcagtcgt tggtcgacgc tggcgtgtac gtgggtggca gtccactcga
      121 gcggcccgac acgaccgcga ctaccctgcg cgtgcgaggc ggcgagcgtc aggtgcaaga
      181 cggcccatac gcggatacca aagagcagct cgggggcttc atggtgctcg agttgccgaa
      241 cctcgacgcg gctctcgatt gggcggcgcg ctgcccggcg gcgtcgtacg ctgcagtcga
      301 ggttcgacct gcggcaaact tggagcgatt tttcgggccc gagcccggca gcttgttccg
      361 tgggtgaagc cgagcgagcc gtcgagtcgg tcgttcgcga atcgtacggc cggctcgttt
      421 cgtttttggc agcgcgttcc ggggacgtga gcagcgcgga ggacgcgctc tccgaagcgc
      481 tcgtggccgc gctccgcagt tggcccaccg cggggatccc aacgcagccg gaagcctggc
      541 tcctgcatgc cgcgcgccgg cgcgtcatcg accagcaccg ctcgcgaaaa ctccgcgacg
      601 agcatgccga gtctttccgg agcgtgctgg aacaagccga agaaatggcg aacggcttcc
      661 agttccccga cgaacgattg aagctcttgt tcgtttgcgc gcatccggcc atcgacgagc
      721 gcgtgcacac gccgctgatg ttgcaagtcg tgctgggcct cgacgccgcc gccatcgcgc
      781 aagctttcct cacgcccgcg gccacgatgg gtcaacgtct gtctcgggca aaggcgaaga
      841 tccgcgacac cagaatcgcg tttcaggtgc ccgccgggga ggagcttgcg ccgcggctct
      901 tcgcggtgct cgaggcgatt tatgctgcat acaatcgcgg ctgggatgac gtcgacggag
      961 ccgacgaaca gcggcgggga ctcaccgatg aagccatgtg gctcgggcgg ctgctggtcg
     1021 agctgatgcc caacgagccc gaagcgctcg gcctactagc gctgatgcat ttttgtgagg
     1081 cgaggcggcc ggcgcgtcgc gactcgaagg gcgaatacgt tccaatctcc gcgcaagagc
     1141 cgcgcgcctg gagcagcgag catctcggcg cggcccaact cctactggct cgggcggcgc
     1201 gactcgggcg catcggccgc tttcagctcg aggctgcggt gcaggccgct cactgcgagc
     1261 gagcgcagac gggacgaacc gattggcccg cgatcgcact tctgtacgaa ggcttggtgc
     1321 gtctcgcgcc gacggtgggc gcgctgctcg ggcgcgcggc ggcggcgggt gaggcgcacg
     1381 gagccaaggc cgggctcgag ctccttttgc aaatccccga gtcggtcgtc gccgagcatc
     1441 agccgtactg gaccgtgcgc gcccacttgg agcagcgctt ggggcggcag gcagaatcgt
     1501 tcgcgcattt cgaacgcgcg ctcgagctca ccgaagagcc cgcagtccga cgctttctcg
     1561 agcgctccat tcgtatccca caggggtgat tgcccggcgg gggatgaagt ggttgctcgc
     1621 gccactcgcg tcgcgcaatc cggtgctcga cgctgagggg aaaagaattc gagcacgctg
     1681 aagagctcag gaagagctga gggtatgcat ttggttgacc ctttgcgagc ccgtggcggc
     1741 cactgcaaag actacgacgc gcagtccgct ttcaacggag ccagtcatga gaactctgga
     1801 aaattccaaa actgtcgctc tcagtttggc tatcagtatc tcggcgtggg cctgtggtag
     1861 ttcgcagggc gacgccgggt cgggcggaac ttcgaatgca tcgaagggtg gggatacgag
     1921 ttcgaccggt ggacagagtg cggcttcggg cggcacgacg gcttcggtgg gctccggcgg
     1981 tgcggctacc tcgggcggct cgggcaacgc gccgggctcc ggtggcgctc ccggctcgag
     2041 cggcgcggcg gctcgcggcg gctcccaggg actcggcggc tcgagcaatt ccggatcggg
     2101 cgggcgcagt gttgggcgcg gcggtggctc ctccgccggc ggcgcttcgg cgggtggcag
     2161 ctcggcgggt agcgccaatg gaggcgcggc tcaaagcggc gacaactgca ccatcggcaa
     2221 atggcccagc gccgatccga cgatgaccgg cccctatgcg accgtcaccg aaaacgaggt
     2281 gggtcccgag gcgggcgcgg tcgatgcgga taccggtatg gtcccgaagt tcacgctctt
     2341 ccgcccgaag gatttgtcgc cgggtggtct ctgccatccc atcatcacgt ggggcaacgg
     2401 cacgggggcg acgcccaacc tctatcgttc gttgctcggg ctcttcgcct cgcacggctt
     2461 cgtggttatc gcctcgaaca gcaaaaacgt ggctcaaggc gatcccaggc cgatgttggt
     2521 tggcgtcacg tgggtgctcg agcaaaacga agatccgacg agcccgctct atcatcacct
     2581 cgatccgacc cacattggag cgactggcca ctcccaaggc gcagtggcca cgagccaagc
     2641 ctcgggcgac gctcgcatca ccacgaacgt ccccatcgaa ggcgcgatgg ttcaacgcaa
     2701 cctccacggc ccagccatgt tcttctgcgg aggaatggac gatgtggtcg ggtgtgacgg
     2761 cgcgaagagc gcgctcaacg cggtgacgac gttgccggcg atgtacgcgg agtacctcag
     2821 cgtcgatcac gggagctgga tgagctacgg caacaagccg agcgtcgttt atggcgcggt
     2881 gacggcctgg atgcgagtgc acctaatgag cgacgactcg cttcgtccct ggttctacgg
     2941 cgagagttgc aagctctgca cggatacggg ctggcaggtg cagcagaaga acatgccgca
     3001 atgaagagcg agtaactcag ggcgcgagct cacttcgcgc atggtcctgc gagcggggag
     3061 ggcgcgcgct cacttcagcg cgtacgagtc ctgcatttgc tgtcgcatcc ccgagacgaa
     3121 gaaggcgcgt ggcgcgcagc gcaattcggc catgaacgcg cggtggcact gtgaatagga
     3181 cccaaacccg acttggcagg ctagctcttt caagttgcgc tcgcctcggt ccacctcgcg
     3241 aatcagtcga agcaggcgcg ctcgggttcg gtactgcacg agcgtgacac cgacgtcgcg
     3301 atggaagtag cggctcacgt ccgacggact cgcgcgtagc gtccgcgcga gcgattctcg
     3361 actcacgtcg gggaagcgag tgagctcggc gagggcgcga cgggtgagca cgtgcaccgc
     3421 gcccccgagc gaggtcgaat tggactgcaa ccactgggcg cgttgccaaa gctcggcgca
     3481 atcgtcgtcg acgcctgcgc gatctacgac gtcgctgcag cgctgaatca ggtgctcgag
     3541 ctcactcggt cggatttgcg agcggagtgg tagcgttggc gcgtcggttg cggcccggcc
     3601 gacctcgctg gcaaggtttg ggtcgacccc gatcgaatac aagtacacgt cgggtgaagc
     3661 gtcgagcagt acgtgatcga ggc
//
LOCUS       DASTKS010000492         3244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1640827, whole genome shotgun sequence.
ACCESSION   DASTKS010000492 DASTKS010000000
VERSION     DASTKS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640827"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..228)
                     /locus_tag="VFQ35_10220"
     CDS             complement(34..228)
                     /locus_tag="VFQ35_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931055.1"
                     /translation="MANERARKALQEARDAIVAVHEGRVERMDGELRREGVRAYVVNR
                     SAERKLTPPGLRVRKRLVAG"
     gene            380..1243
                     /locus_tag="VFQ35_10225"
     CDS             380..1243
                     /locus_tag="VFQ35_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9931056.1"
                     /translation="MVSDDAVRRARVASGAVCFLMMPMKLLILDVDDTLFDWLTMWSE
                     SFGAALATLLSATRLSETELLAALRALNRSARTSERGFTHDDAEMLGVNRDTVAALAS
                     QFENDSRQRTQLFPDVLGTLAELKRRGVTLVAHTDTPVEVGADRFVQLGLDGIVDALF
                     ATAVKGAVLHRRHLNRPQRARLFEVPHPKPDRRAIGQILAEYGRVASECIYVGDSKMK
                     DIAMARAAGVTDIFAAYGCRRTSTSYELLRKVSHWTDDDIAYEKSLLAVEPTHSLEQF
                     SDILRFVPSSP"
     gene            1360..1569
                     /locus_tag="VFQ35_10230"
     CDS             1360..1569
                     /locus_tag="VFQ35_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931057.1"
                     /translation="MTNGTIPETGALAPITDPWDHLAELKARRRELDDAFMRSLERLE
                     KLLLGESPTSSPATQHLQGGGFPDE"
     gene            1562..1792
                     /locus_tag="VFQ35_10235"
     CDS             1562..1792
                     /locus_tag="VFQ35_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931058.1"
                     /translation="MNDQNREEARLVAHEEARLEAQMERMTGRARERAEDRAEDDARS
                     EADDDGAFERLYIESFDRHYASELARLERARP"
     gene            complement(1855..2451)
                     /locus_tag="VFQ35_10240"
     CDS             complement(1855..2451)
                     /locus_tag="VFQ35_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931059.1"
                     /translation="MARREHEQFDLRVDVGMRDEPTELVVGEHGARASVLFHVGHDGA
                     LGDVLGDELLAPRRFEYAQDRRAHVHDAFARVAVAVLLGDRAFDGAVENGLEHARANG
                     AEFERADLLLPHALEVPSLVRRARELTPLATLEPLFRVRGNRLGRLAGVGGKGVARGV
                     FGDEPLHLAARRGLAVRFEVEPTAHALAVDGDGELAPP"
     gene            complement(3107..3178)
                     /locus_tag="VFQ35_10245"
     tRNA            complement(3107..3178)
                     /locus_tag="VFQ35_10245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3144..3146),aa:Gly,seq:gcc)
BASE COUNT          611 a         1076 c         1047 g          510 t
ORIGIN      
        1 gatgagaact gcaccctcag ataaggtgtg ggcttagccc gcgacgaggc gcttccgaac
       61 tcggagcccc ggcggcgtca gtttgcgctc ggcggatcga ttcacgacgt acgcacgcac
      121 gccctctcgc cggagctcgc cgtccatgcg ctctacgcgc ccctcgtgaa cggccacgat
      181 ggcgtcccgg gcctcttgta gtgccttgcg cgctcgttca tttgccatcg gctgttcctc
      241 caggattacc ggactctacg aagcaacggg gaattttcaa cgcgcgagcg tccaaaaacg
      301 tttgccaaga cctaaagaaa gtcctagccg cgacggtgtc ccgagccccc atgcccaacg
      361 tattgggtat gcggccctcg tggtgtcgga tgacgcggtc cgtcgggcgc gtgtcgcgag
      421 cggcgctgta tgctttctga tgatgccgat gaagctcctc atcttggacg tcgacgacac
      481 gctcttcgac tggctcacaa tgtggagcga gtcgttcgga gctgcgctcg caacgctgct
      541 gagcgcgacg cggctgtcag agaccgagct tcttgccgcg ttgagagcgc tcaaccgaag
      601 cgcgcgcacg tctgagcgcg gattcacgca cgacgacgct gagatgctgg gcgtcaaccg
      661 ggacaccgtc gcggcgctcg cgtcgcagtt tgaaaacgat tcccggcaac gcacgcaact
      721 atttcccgac gtgcttggga cgcttgcgga gctcaagcgt cgcggagtga cgctcgtggc
      781 tcataccgac acgcctgtcg aagtcggcgc ggatcgcttc gttcagcttg gcctagacgg
      841 gatcgtcgac gctcttttcg cgaccgccgt gaagggagct gtgttgcatc gccgtcacct
      901 aaatcgaccg cagcgcgcgc ggctcttcga ggtgccacat ccgaagccag atcgacgcgc
      961 gatcgggcaa attttggctg aatacgggcg tgtcgcgagc gaatgcattt acgtcggcga
     1021 cagcaagatg aaggacatcg cgatggctcg agccgccggc gtcaccgaca tcttcgccgc
     1081 gtacggatgt cgtcgcacca gcacttcgta cgagctcttg cgcaaggtga gtcactggac
     1141 cgacgacgac atcgcctacg aaaagtcgct cctcgcggtg gagccaacgc attcgcttga
     1201 gcagttcagc gacatccttc gcttcgtacc gtcgtcgccc tgagtcgagc gagcttgacg
     1261 agccgagctc gaacgcgaat cctgaacgct caaccccaat aggtggggcc cgcggtgctt
     1321 gcaacacccg gacccctggc caccgaaagg aacctttcga tgacgaacgg cacgataccg
     1381 gaaacgggtg cgctcgcgcc cataactgac ccctgggacc atctcgcgga gctcaaggcc
     1441 agaaggcgcg agctcgacga cgcgttcatg cgttcactcg agcggctgga aaagctgctg
     1501 ctcggtgagt ccccaacgag ctcgcccgcg acgcagcatc ttcaaggtgg aggttttccc
     1561 gatgaatgac caaaaccgtg aagaagctcg actcgtcgcc cacgaagaag cacgcctcga
     1621 ggcgcagatg gagcgaatga ccggacgagc acgcgagcgc gccgaagatc gcgccgagga
     1681 tgacgcgcgg tccgaagcag acgacgacgg cgcgttcgag cgtctctaca ttgaatcgtt
     1741 cgaccggcac tacgcgagcg agctcgcgcg actcgagcgg gcacgtccgt gagcgaaacg
     1801 caaaagcgca agggcggacg cccgcgcacc ggctaccttg agttccgcgg caagtcatgg
     1861 tggtgccagc tcaccgtcac cgtcgacggc gagagcgtgc gccgttggtt caacctcgaa
     1921 acggacagca agcccgcggc gcgccgcaag atgcagcggc tcatcgccga acacgccgcg
     1981 ggcaacaccc ttcccaccga cgccggcaag acgcccgaga cggttgccac gtacgcggaa
     2041 gagtggctca agggtcgcga gcggcgtgag ctcccgagcg cggcgaacga gcgacggtac
     2101 ttcgagcgcg tgtggaagaa ggagatcggc gcgctcgaac tcggcgccgt tcgcgcgggc
     2161 gtgctccaag ccgttctcga ctgcgccgtc gaaggcacga tcaccaagaa gaacggcgac
     2221 cgctactcgc gcgaaagcgt catgcacatg cgcgcgacga tcttgcgcat actcgaatcg
     2281 gcgtggcgcg aggagctcgt caccgagaac gtcgccaagc gcaccgtcgt gcccgacatg
     2341 gaagaggacc gacgcccgcg cgccgtgctc accgacgacg agctcggtcg gctcgtcacg
     2401 catcccgacg tcgacgcgga gatcaaactg ctcgtgctca cgtcgcgcca catcggcggc
     2461 cttcgcacgg gcgacctgaa ccggatggat tggtcggcct tctcgccgaa cttcaagacg
     2521 tgcacgttcg tccgtcgcaa gactcggaag aagcggcccg agcccgagac gcacgctgtg
     2581 ccggaggcgg tgcgcccgtt cctcgacgcg tggtggcgcg ctgcaggctg cccggcgacc
     2641 ggccccgtgt tcccggcacg ccgcggcgac cgggtgggga aggagaagaa gcaggcgaag
     2701 cagagctacg ccgaacgcct ccgccgcgag cttcgaatcg ccttcggcat cgacgcgcgg
     2761 gaccccttga ccggcgcctg gacggcgggc gcccgcgcgc ccacgcctcg ggagcgggaa
     2821 ctgctcgagg ggtcagccac cgtccagccg gtggacttcc acagcacccg ccgcgcctac
     2881 gcgacgaccc tggctcacgt tggggtgaac gagcagacgg cgatgcagct caccgggcac
     2941 tcggactcga agacgcaccg gcgctaccag gcggcggacc gggggctcac cctgccgccc
     3001 gaagccgtcg tcgtggtgcc gcccgacgcg atcgccgttt tcgctgcaaa caggaatcgc
     3061 accagtcgtg gtgcgattcc tagggactcc gaagtgctaa gtccggagcg ggagaaggga
     3121 tttgaaccct cgacgtccac cttggcaagg tggcactcta ccgctgagtt actcccgcgg
     3181 aagagccggc ggtgtacctc ccgctctctt tcgtgtcaag cacttgccgg aggtcggccg
     3241 gacg
//
LOCUS       DASTKS010000493         9862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_525703, whole genome shotgun sequence.
ACCESSION   DASTKS010000493 DASTKS010000000
VERSION     DASTKS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525703"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFQ35_10250"
     CDS             <1..769
                     /locus_tag="VFQ35_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET9931060.1"
                     /translation="IVPPAPADPHTHADMTMMQTMSGSELDQMFLIDMIAHHAAGLAP
                     AQRAQSLLVRADLKQLAMSIFSAQAAEIGTMKTMLVSMGVDDPGADLATDVAGRADFG
                     LAGDRRVPLTPGDLTFIDFFVPHHKMAIEMAKLAIANGSADVKQMATMMRDTQQQEVD
                     IMLAVRLELAGSATVPEPPRDPMMMAHMEHMKTLSGASLDQMLLEQMIPHHAAGIPTA
                     HRANPHIQRPELKKLASDIFNAQAREIGEMETMLEAP"
     gene            complement(871..1278)
                     /locus_tag="VFQ35_10255"
     CDS             complement(871..1278)
                     /locus_tag="VFQ35_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931061.1"
                     /translation="MSQFKFLFVSALAAALALPLAARADSAECELHAFKANRVEPLRV
                     QERIGRGTIEKLAGAQVFVPAQKGLTAEWLRARVEQHISGMSSQGMANCPLGVKGVRV
                     TVASGGTGFWVKLQAKDSETAHEVLRLARAAVR"
     gene            complement(1977..3224)
                     /locus_tag="VFQ35_10260"
     CDS             complement(1977..3224)
                     /locus_tag="VFQ35_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET9931062.1"
                     /translation="MELSDGELLTATRRLVGRSNQLLALLLGHLAEVEARGLHRTRAC
                     ASLYTYCIYELRFSEDEAFRRVAAARLVRRFPALLDAIASGELHLTGLLMLGSHLTAE
                     NLSEVLARAKHRTKKEIARLVRQLDPLPDVPARIEPLGPAPARVTPSTPTFRQFIDSL
                     CPVRELAAGDRPRDWIDCGERGHPEDSGECSQEVTPTQALPAPGAPTQLEPQRYRVQF
                     TAGEEYVKLVEAAQALLSHSAERVGIEELQLRAMRALVAELERRKRAVPKRDVTKCDV
                     THVSGEHPRESGSAGRVAATDVVAATDVADSPHTRRRGRHIPASVRRAVFERDEERCA
                     YIDGSGQRCRETHRLELHHVNAFALGGAHTLENLALRCRAHNALAAEHDFGEDFVRQR
                     RDSSRHESYRQQTEPNELRPGDG"
     gene            4216..4875
                     /locus_tag="VFQ35_10265"
     CDS             4216..4875
                     /locus_tag="VFQ35_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931063.1"
                     /translation="MIPILACGGNVGDSSSKGGDPQAGGAVAVGGAVANGGGVAVGGA
                     ISGGGTTGILPGEKALEALSADELAQLRQAVDTQISTELGEIPLPLRCHYQSVLQASY
                     IAGAGSSGTSDDVREACASGDAICVRQGEAPYIDSKPLRPTTFVSADTFSHCSATVRD
                     YQTCTTDLVRAEADVMMSLPACDTLDVHAYPKGLGTVPVDMVEPSSCMALYAQCPGLN
                     R"
     gene            4872..6161
                     /locus_tag="VFQ35_10270"
     CDS             4872..6161
                     /locus_tag="VFQ35_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HET9931064.1"
                     /translation="MSGRLWNVRKRRAPFTLALALASALVLAPVFFACSPPVSAPPSA
                     AAIQPRPPLSARVAAPASSASGHPAAPVSSAPEAPAPSEPPPLLIEQLRSDRQWTGVA
                     ASLDPSRRLFVSYPRWSDKVPVSVEEISFGVASDERGSVPFIGVGPYPDEEWNAWRPG
                     LDPRKHFVAVQSVLVTEGTLWVLDAGNPKFGGVVRGAPKLVVIDLTTDAVRDVIPFDA
                     KIASGASYLNDVRIDLSRGFAYITDSGKGGLVVLDLLTKKAQRVLDNSPSTHAEDTVL
                     TIEGAPWRRPDGSNPKVHVDGIALSPDGEWLYYQALTGRTLYRIATDVLRSPRFSNDD
                     RARWVQVVGTLGPADGLATMADGSIIHTSLEANALVLVRPDGTTKTLVQDPRLAWPDS
                     LAFGRFGELWVTTSRIHRGGNPGEPYGLWVVPQWAFK"
     gene            complement(6445..7218)
                     /locus_tag="VFQ35_10275"
     CDS             complement(6445..7218)
                     /locus_tag="VFQ35_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931065.1"
                     /translation="MIGTIRASTFACATIWCVIASAQTKASEPPSPFPTRISNCPNEL
                     ASALPSVIRLEVDALLRERGLASPPESVAVQCEGDSARITVSAGGATRDTNVDLSPLA
                     APHRARAVALAAAELLHSLANAADDEASKAPSPAPPPTKPPPRPTTSTSPPSTPAPPP
                     RHASTAPALLVGALGEWLGKPATPLFGARVALRYPLTALVVPALSADASFGSASTTSA
                     RVAARTFGAGAHLYFSGTTGNFRWSAGPGARIAGAVLVG"
     gene            complement(7265..8422)
                     /locus_tag="VFQ35_10280"
     CDS             complement(7265..8422)
                     /locus_tag="VFQ35_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9931066.1"
                     /translation="MSEHQRIELRGLKVGWNAARTERLLDQVHSQLEGQQRRWRAALA
                     SASLVVLVLIGGAVWHFQRAPGVAATPKSAPSIVVGAPDPSALVLRDGSEIQLDPRTS
                     TVRVAEESASKVRIELERGSARYEVTPHPERSFEVHAGSVTVTVVGTAFLVERRGEST
                     WVEVSRGKVRVSWNESEPVFLVAGESGLFPRGALPSADAASPSDNHEGNAAPEASDKA
                     PEASDKAPAATRAQQASVAYRAHIARHDYRGAYAVLSQNPGLAGDTVQDLLLAADVAR
                     LSAHPADAVPYLQSIVRNHAQDARAPMAAFTLGRTLSGLGRTREALNMFGKVRSSWPK
                     SSLAEDALIRQAEASAQLGDYAAAARFAAQYDREYPQGRRRAEVRRYAHLE"
     gene            complement(8419..9051)
                     /locus_tag="VFQ35_10285"
     CDS             complement(8419..9051)
                     /locus_tag="VFQ35_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931067.1"
                     /translation="MSRERALRHAQPADESVGDSAAHFHGLRSRPEPAVDAQREKEAE
                     AEAEAAASLDFDDIFRRYAPYVGSLVLKLLGRPGDVDDVVQDVFMQAHRGLSRLRDPA
                     AVRPWLRRITIRRTRRFLRKRWVLRWFSEASVETQTDLADPSASPEERAQLAHIYRRL
                     ELMARDERLAWVLRFVEGETLEGIAELLSCSVSTVQRRLRAAEACMGEIR"
     gene            9188..>9862
                     /locus_tag="VFQ35_10290"
     CDS             9188..>9862
                     /locus_tag="VFQ35_10290"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HET9931068.1"
                     /translation="MSSWLKNLRVLTSLPLALALASALAACRGTEGDVLRTRPDMSGH
                     DDAGTLRPRPTPLSSWQIQLSGTLVTSLDVKLYTVDLDTPSTRIEALHAAGRIVLCYF
                     SAGTRESFRSDAAEFPAETLGSALPDYPNERWVDVRDDRVRALMRARIAKAAEAGCDG
                     IHPSGLAAFLEDTGLAFGRADQLGYNRLLSTETHARGLSIGLVEGDLTLTEAQVSDFD
                     WVVVWSC"
BASE COUNT         1696 a         3144 c         3302 g         1720 t
ORIGIN      
        1 catcgtgccg cccgcgccgg cggatccgca cacccatgcc gatatgacga tgatgcagac
       61 catgtctggc agtgagctcg accaaatgtt tttgatcgac atgatcgcgc accacgcggc
      121 tgggctggca cctgcacagc gtgcgcagtc gctgctggtg cgagccgacc tgaagcagct
      181 cgcgatgagt atcttctcgg cacaagcggc cgagattgga accatgaaga cgatgctcgt
      241 ttcgatgggc gtcgacgacc cgggtgccga tctggcgacg gatgtcgcgg gccgcgctga
      301 ctttggactc gcgggagacc ggcgcgtccc gcttacacca ggtgatctga cgttcatcga
      361 cttcttcgtt ccgcatcaca agatggcgat cgaaatggcg aagctggcga tcgcaaacgg
      421 cagcgccgac gtgaagcaaa tggccaccat gatgcgcgac acacaacaac aggaagtcga
      481 catcatgctc gccgttcgac tcgagctcgc gggcagcgcg acggttccgg agccgccgcg
      541 tgaccccatg atgatggcgc atatggagca catgaagacc ttgtccggag cgtcgctgga
      601 ccaaatgttg ttggagcaaa tgattccgca tcacgctgcc ggcatcccca ccgcacaccg
      661 ggccaacccc catatccagc gccccgagct gaagaagctc gcctccgaca tcttcaacgc
      721 tcaagctcgc gagatcggcg aaatggagac catgctcgag gctccatgag cgcatggaga
      781 tgagtctccc tcgcccgccg cgcattctcg catgaatggc gatgcggctc cgcgcgggca
      841 ttcctcgcgg agccgcagcc cgcggccgcg tcaacgaaca gccgctcgtg caagacgaag
      901 gacctcgtgg gctgtctcag aatccttggc ctggagcttc acccagaagc cggtgccgcc
      961 cgaagccacg gtgacgcgaa cccctttcac gccgagaggg caattggcca tgccttgcga
     1021 ggacattccc gagatgtgtt gctcgacccg cgcgcggagc cattcagcgg tcaggccttt
     1081 ttgcgcaggc acgaagacct gcgcaccggc aagcttctcg atcgtgccac gtccaatgcg
     1141 ttcctgaacg cgcagaggct cgacgcggtt ggccttgaag gcatggagct cgcactccgc
     1201 agaatctgcg cgagcggcga ggggaagggc gagagctgca gccagagcgc tgacgaacag
     1261 aaacttgaat tgggacatgg cacgttctcc tgattgcgag gtgcaatcgt cgttgttcgc
     1321 tggctatagg cagcgattcg gcaaagcgtg acccgcgcgc gagcggattc tcgtcggcgc
     1381 ctcgcatggc cacgtacaac gtccgccaac tacgacaagg agcgtgcaat ggacccgatt
     1441 gccgtcagcc cctgggcgaa acgcgccatg gcctcccgat ggctcgagag ctcgccatag
     1501 ggcaagtagc gcgcgcaaat cggcaagtcc gttgcgcgag tagcacggca cttttgctgc
     1561 ttccgtacct ggccgaggtc acttcccgct tgccgcgctt cgcgacgagg cgaaggccac
     1621 tgagcgcatt ggaagcgcta acccgctcga gctcgaggcg cagcgccggc gtaatcggtg
     1681 cgaccaccac cagggcggtg cgctcgtcaa tcgcggtgct cacgctgccg gcgccgaaca
     1741 tcgtttgggc aagttggtgc gcgacatcgt tgcacaggcc gcgcaagcgg gcgcagtccg
     1801 aaacgacgtc ggcccggaag agctcgcgca gttcttctcg cacgctctca ccgccgccgg
     1861 ccaggcttcg tccaaggcgg cggtgagaac ggctggttgc gggatcgcgt tgtcgagctc
     1921 ttcgagggct tgcgttggag gcttgagggc ggggtaaacc gctgggcgct gggcggttaa
     1981 ccgtccccag gtctcagctc gttcggttcg gtttgctgtc tgtacgattc atgtcggctc
     2041 gagtcgcgac gttgccgcac gaaatcctcc ccgaaatcgt gttcggcggc gagtgcgttg
     2101 tgcgcgcggc agcgcaacgc aaggttctcc aacgtgtgcg cgccgcccag ggcaaaggcg
     2161 ttcacgtgat ggagctcgag ccgatgcgtc tcccggcagc gctgccccga gccgtcgatg
     2221 tacgcgcagc gctcctcgtc gcgctcgaac accgcgcgcc ggacggaggc cggaatatgc
     2281 ctaccgcgcc ggcgcgtgtg cggcgagtca gccacgtccg tcgcggcaac cacgtccgtc
     2341 gcggcgaccc tgcccgctga ccctgactcg cgtgggtgct cgccggaaac gtgcgtcacg
     2401 tcgcacttcg tcacgtcgcg cttcgggacc gcgcgcttcc ttctttcaag ctctgcgaca
     2461 agagcgcgca ttgcccggag ctgcagctcc tcgatgccga ctcgctcggc ggagtgagac
     2521 agcagcgctt gggccgcttc tacgagcttg acgtactcct ctccagccgt gaattgaact
     2581 cgatagcgct gcggttcgag ttgcgtgggc gctccgggag cggggagcgc ctgcgtagga
     2641 gtcacttcct gcgagcactc ccccgagtcc tctggatgcc cgcgctcgcc acaatctatc
     2701 caatcgcgag gccggtcacc ggcggcgagc tcacgaaccg ggcacagcga gtcgatgaac
     2761 tgcctaaacg tcggagttga aggcgttacc cgcgctggcg cgggtccgag cggttcgatg
     2821 cgagctggca cgtccggcag cgggtcgagc tgccgaacca ggcgggcgat ttccttcttg
     2881 gtccggtgct tagcccgggc cagcacttcg ctcaggtttt cggcggtgag gtgagagccg
     2941 agcatcaaca aacccgtgag gtgcagctcg ccggatgcga ttgcgtcgag cagcgcggga
     3001 aagcgtcgaa ccagtcgtgc cgcagccact cgacgaaagg cctcgtcctc cgaaaaccgt
     3061 agctcgtaaa tgcaataggt gtagagactc gcgcaggcgc gcgtgcggtg gagaccacga
     3121 gcttcgacct cggcgagatg cccgagcagc agcgcgagca gctgattgga gcgacccacg
     3181 aggcgtcgcg ttgcggtgag caactcgcca tccgagagct ccaccggtag agttaccgtg
     3241 accggagaaa cggaaccgta accgcccaca attgccggtg cgtttatgaa ccgcacctta
     3301 caccctgatt ttcggagctc cgtattcgca cgctcgcgag ctttcgggag cagagacgaa
     3361 gttcgaagtg tcgagccgtg cgaggactgc gctacgagct tcgtgcttgc gctaacacca
     3421 aacgtcgacg acgcgtttcc gaggcggtgt ccctgacgtg cctggtgcgc atggtgcgct
     3481 gcagcctttg ctacgtcggc ttcaaggagc gcaagcctgt ctgcgctgac ctgcgtcgca
     3541 cacaccgcgc tgacctgcgg cgcatccgca cggcgctgac ctgcgtcgca cacaccgcgc
     3601 tgacctgcgg cgcatccgca cggcgtaaac ggtgccgagt gcggaggatg cgcccgagga
     3661 attcgaccgc cgttgggggc gcagtttgac gcaaacgcgc gttagctcct cttcctcgcg
     3721 ctgcgcagcg ccgaagcggt ttggctccgg gcgttccgcg ccgcgggctt tcgccgacag
     3781 tgcgaccgag ccatgttgcg attcgcgatg tggtctcgac tgagcaccct cgccgcgatt
     3841 ggacttcgcg gaggtgcgcc aaagggtgcg gtgagtgaaa gcgacttcgc gcgggttcgc
     3901 cgagcggtgc ggtgcctgga accgacttcg cgggggtcgg cgaagggtgc ggtgaatgga
     3961 accgacttcg cgcgggttcg ccgagcggtg cggtgagtgg aaccgacttc gcgcaagtgc
     4021 gccgaccgaa gcgacgtagc cgctcgtcac acggcggcgc gccttacgtg gcggctgcct
     4081 cgcgtgcggt tggcgccaac agcgacagcg cccgtgctcg ctatggatcg agcgaacgcg
     4141 tggttggaca attcggtggt aacgggtgta aatatacgca tggaattggg gcgcgttgct
     4201 gtagttgtca tggtcgtgat tcccatactc gcctgcggtg ggaacgtcgg cgactcatcg
     4261 tcgaagggcg gggacccgca ggctgggggc gccgttgcgg ttggaggcgc ggtagcgaat
     4321 ggtggcggcg ttgcggtggg tggcgcgata tcgggaggcg gcaccaccgg tattctgcca
     4381 ggtgaaaagg ccctcgaggc tctcagcgca gatgagcttg ctcagctgcg acaggcggtc
     4441 gatacgcaga tcagtacgga gttgggcgag atcccgctcc ctctccggtg tcactaccag
     4501 tccgtgttgc aagcgtccta tatcgccggc gcgggctcat cggggacgag tgacgacgtc
     4561 cgggaggcgt gcgcatcggg cgacgccatt tgcgttcggc agggcgaagc accctacatc
     4621 gattcgaaac cacttcggcc aacaaccttc gtgagcgccg acactttcag tcactgtagc
     4681 gcaacagttc gagactacca aacgtgcacg actgatttgg ttcgggctga ggcggacgtg
     4741 atgatgtcgc tgcccgcgtg cgacacgctc gacgtacatg cgtacccgaa aggattaggg
     4801 acggtgcccg tggacatggt tgagccttcg agctgcatgg cgctctacgc gcagtgtccg
     4861 gggctcaatc gatgagcggt cgtctttgga atgttagaaa gcggcgtgca ccgttcacgc
     4921 tggcgctggc gctggcgtcc gcgctcgtgc tcgcgccggt gttctttgcg tgttctcctc
     4981 cggtctccgc gccgccgtcg gccgctgcga ttcagccacg tcccccgctc tcggctcgag
     5041 ttgcggctcc cgcgtcgtcc gcctccggtc atcctgctgc gccggtgtcg agcgcgccgg
     5101 aggcccccgc gccgagcgag ccgcctcccc tgctgatcga acagctgcgc tctgaccggc
     5161 aatggacggg cgtggcggct tctctcgatc cgagtaggcg gctgttcgtg agctacccgc
     5221 gctggagcga caaggtccca gtctcggtgg aggagatctc tttcggagtc gcgagcgacg
     5281 agcgaggctc ggtcccgttc atcggcgtgg ggccctatcc ggacgaggaa tggaacgcct
     5341 ggcgccccgg gctcgatccg cggaagcact tcgtcgctgt acagagcgtg ctcgtaaccg
     5401 agggcacttt gtgggttctc gacgccggaa acccgaagtt cggtggtgtg gtgcggggcg
     5461 cgccgaaact cgtcgtcatc gatctcacca cggacgcagt gcgggacgtc attccattcg
     5521 acgcgaagat cgcgagtggt gcctcgtacc tcaatgacgt gcgcatcgat ctttctcgag
     5581 gcttcgccta catcaccgac tcgggaaagg gcggtttggt cgtcctcgat ctcctgacga
     5641 agaaggcgca gcgcgtcctc gacaatagcc cgtcaacgca cgccgaagac accgttttga
     5701 ctatcgaagg cgctccctgg cggcgacccg atggctcgaa tccgaaggtc cacgtggacg
     5761 gcatcgcgct ctcgcccgac ggagagtggc tctattacca agcgctcacc gggcgcaccc
     5821 tctatcggat cgcgacggat gtgcttcgca gtccgagatt ttctaacgac gatcgcgcac
     5881 gttgggtgca ggtcgttggc actctcgggc ctgccgacgg ccttgcaacg atggcggatg
     5941 gcagcatcat ccacacgagt ttggaggcga acgcgctggt cttggtgcgg cccgacggaa
     6001 ccacaaaaac gctggttcaa gatccgcgct tggcgtggcc cgacagcttg gctttcggga
     6061 ggtttggaga gctttgggtg accacttcac ggattcaccg cggtggaaac ccgggtgaac
     6121 cctacgggct ttgggtagtg cctcaatggg cgttcaagta agtcaagcga tcgacgtcgc
     6181 gcgagcacgc agccccattc acagctcgag gcccacctcg atacagagcg acacccacgg
     6241 tcctgtcagc gagtagaccg aatcgccgcc atcgcgcaat ccgcgtagcg ggagcgtgac
     6301 gacgccagca ccgagttcga aagcgaggag cggtgagcgc gcggaggcgg cggatgacgt
     6361 cgcgtagtcg atgcgcgcgc gcgcttcggg tccgccccag atcccggaga gcgtgcgtcc
     6421 gtcgagcgtc gaatcggccg ctggctagcc aacgagcaca gccccggcga tgcgcgcgcc
     6481 gggccctgcc gaccagcgga agttcccagt tgtgccgctg aagtagaggt gcgctccggc
     6541 gccgaaggtt cttgcggcga cacgcgcgga cgtggtggaa gcgctgccga acgatgcgtc
     6601 ggcagagagg gcagggacga cgagcgcggt gaggggatag cgcagcgcga cgcgtgcgcc
     6661 aaagagcggc gtcgccggtt ttcccagcca ttcaccgagc gcgccgacga ggagggcggg
     6721 cgccgttgac gcgtggcgtg gaggtggcgc gggcgttgag ggaggcgacg tgctcgtagt
     6781 cggacgcggc gggggtttgg ttgggggagg agctggggat ggcgctttgg aggcttcgtc
     6841 gtctgcggcg ttggcgagcg agtgcagcag ttcagcggcg gcgagtgcga cggcacgggc
     6901 gcgatgcggc gcggcaagcg gactcagatc cacgttggta tcgcgcgtgg ctccgccggc
     6961 gctcaccgtg attcgtgcgc tgtcaccttc gcattggacg gcgacgctct cgggaggtga
     7021 ggcgaggccg cgttctcgca agagcgcatc gacttcgagt cgaatgaccg aagggagcgc
     7081 actcgcgagc tcgttcgggc agttgctgat gcgcgttgga aagggggagg gaggctctga
     7141 agcttttgtc tgcgcgctcg cgatgacgca ccaaatcgtg gcgcacgcga acgttgaagc
     7201 acggatcgtc cctatcactg gtccgcgact acagtagcgc acctactcga ggcgagcgta
     7261 gcgactattc gaggtgagcg tagcgacgca cttccgcgcg gcgccggcct tgggggtatt
     7321 cgcggtcgta ttgagccgca aaacgcgccg cagcagcgta gtcgccgagt tgtgcagacg
     7381 cttcggcctg gcggatgagc gcgtcttcgg cgagtgagct cttgggccaa gagctgcgca
     7441 ccttaccgaa catgttcaac gcttcgcgag tgcgaccgag gccactcaag gttcggccga
     7501 gtgtgaatgc ggccatcggc gcgcgcgcgt cttgcgcgtg gttccggacg atcgattgca
     7561 gatacgggac cgcgtcggcc gggtgcgcgg agaggcgagc cacgtccgcc gccagcaaca
     7621 ggtcttgcac ggtgtcaccg gccaaaccgg ggttctgcga gaggacggcg tacgcgcctc
     7681 ggtagtcgtg gcgggcgatg tgcgcgcgat aagcaacgct tgcctgctga gctcgcgttg
     7741 cggccggcgc tttgtcgctg gcttccggcg ctttgtcgct cgcttccggc gccgcattgc
     7801 cttcgtgatt gtccgatggc gacgccgcgt ccgcgctcgg caaagctccc cgcgggaaga
     7861 gaccgctctc gccggcgacg aggaagactg gctcgctctc gttccacgac acgcgcactt
     7921 tgccgcgcga aacctcgacc caggtgcttt cgccgcgccg ttcgaccaga aacgccgtac
     7981 ccaccaccgt caccgtaacg gagcccgcat gcacctcgaa gctccgttct gggtgaggcg
     8041 tgacctcgta gcgcgccgag ccgcgctcga gctcgatgcg tactttgctt gcgctctctt
     8101 ctgcgacgcg gacggtgctc gtgcgcggat ccaattggat ctcggagcca tcgcgcaaca
     8161 cgagcgcgct cggatccggc gctccaacca caatcgacgg agcgcttttc ggcgtggccg
     8221 cgacgcccgg cgcacgttga aaatgccaaa cagctccgcc gatcagaacc agcacgacga
     8281 gcgatgcgga cgccagcgcg gcgcgccacc tgcgttgttg accttcgagc tgagagtgca
     8341 cctggtccaa caaccgctcg gtgcgcgcgg cgttccagcc gactttgaga cctcgcaatt
     8401 cgatgcgctg atgctcgctc atcggatttc ccccatgcag gcttcagcgg cgcgcaggcg
     8461 tcgctgcacg gtggatacag agcaactgag gagctcggcg atcccttcca aggtttcgcc
     8521 ctccacaaag cgaagcaccc acgcgagccg ctcgtcacgg gccatcagct cgagccggcg
     8581 gtaaatgtgc gcgagctgag cgcgctcttc cgggctcgca gagggatcgg cgagatccgt
     8641 ttgggtctcg acgctggcct cgctgaacca acgcagcacc caacgtttgc gcagaaaacg
     8701 gcgtgtgcgt cgaatcgtga tgcgtcgcag ccagggccgc accgcagcgg gatcgcggag
     8761 cctcgaaagg ccgcgatgcg cctgcatgaa cacgtcctgc acgacatcgt ccacgtctcc
     8821 cgggcgaccg aggagcttca acaccagcga gcccacgtag ggcgcgtagc ggcggaagat
     8881 gtcgtcgaag tcgagcgatg ccgccgcttc ggcctctgct tccgcttcct tttccctttg
     8941 cgcgtcgacg gccggctcgg gtcgactgcg aagcccatga aagtgcgcgg ccgaatcgcc
     9001 cacgctctcg tcggctggct gggcatgacg aagggcccgt tcgcgcgaca cgccgcagtc
     9061 tcgcacgcga aggtggccgg gcgccagccc gaaaggctcg gacgcgcgtg aactcgaggt
     9121 gcgaacagaa agaaccaaaa cagctatttg cccagtgccc ctgaaaaggc aaaacctggt
     9181 cacagcaatg tcgtcgtggc tgaagaacct gcgcgtgctc acgagcctgc cgttggcgct
     9241 ggcgctcgcg tcggcgctcg ctgcgtgtcg gggcaccgag ggcgacgtat tgcgcacccg
     9301 gcccgacatg tcagggcacg acgacgcggg aacgctgcgg ccgcgaccga cgccgctcag
     9361 ctcctggcaa atccagctgt ccggcacgct cgtgacgtct ctcgacgtaa aactttacac
     9421 ggtggacctc gacaccccga gcaccagaat cgaggcgttg cacgccgccg ggcgcatcgt
     9481 gctctgttac ttcagcgccg gcacgcgcga gtcctttcgc agcgacgccg ccgaattccc
     9541 tgccgaaacg ctgggctccg cgctccccga ctacccgaac gaacgttggg tcgacgttcg
     9601 cgacgaccgc gttcgcgccc tcatgcgcgc tcgcatcgcc aaagcagcag aagcaggctg
     9661 cgacggcatt cacccaagtg gcttggccgc ttttctcgaa gataccgggc tcgcattcgg
     9721 gcgcgcagat cagctcggat acaatcgctt gctcagcacc gaaacgcatg cgcgcggcct
     9781 ttcgatcgga ctcgtcgagg gcgatctcac tctgaccgag gcgcaagtca gcgacttcga
     9841 ttgggtggtg gtctggagct gc
//
LOCUS       DASTKS010000494         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1640907, whole genome shotgun sequence.
ACCESSION   DASTKS010000494 DASTKS010000000
VERSION     DASTKS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640907"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VFQ35_10295"
     CDS             complement(<1..1351)
                     /locus_tag="VFQ35_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931069.1"
                     /translation="MPAPPIDPAQQDPERDRIVTEEQECLTKVLQHLSEHKQKERARS
                     GHESGDYDARLLDLRDQIASARLEDIPPLVQEMERLQSLASRLRQVTETEIDARSPYF
                     GRLILEEGDRRREVLIGRGTHLDTRSGIRIVDWRDAPVSRLFYRYEEGDGYDEEFGGR
                     EVSGTVSVRRSVVISDAELRRVGSPQGSFARNRDGSFRRLSDTATRLAGGAGSAIRAE
                     QHHRPGKLGTGQEGGEEKQLKEITPLIDRRQFELITRPDSGLVVIQGGAGSGKTTIGL
                     HRLAYLAFQDSRRFRPDRMLVIVFNDALARYIGGVLPALGVHGVGIRTYEEFASRLRQ
                     ALFPRLPSRYREDTPAVVTRMKKHPAMLLAIESIARRLNARFDAEVRAALTPDPAGSS
                     LLAHWEQTADRVPAHRLHALSAKLESESKHLSTNARVSLERTVKVGLQITRDVISHWA
                     "
     gene            1536..2513
                     /locus_tag="VFQ35_10300"
     CDS             1536..2513
                     /locus_tag="VFQ35_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9931070.1"
                     /translation="MLLEAARSPLVRRQVHVPEPRGDEILIKVRCCGVCRTDLHVLDG
                     ELPGPKLPLILGHQIVGTIVAMGHEVEGFVESQRVGVPWLGRACGDCRYCTSGKENLC
                     ERALFTGYQLDGGYAEYVVAQAKFALPLPGSFPDLQAAPLLCAGLIGYRALVMSGDAE
                     CLGLYGFGSSAHIVAQVARHQGRRVFAFTRPGDFAAQDFARRLGAEWAGGSDEVPPCT
                     LDSAIIFAPAGTLVPRALRAICRGGTVVCAGIHMTDIPSFPYELLWGERTICSVANLT
                     RRDGVEFLELAPRVPIRTEVRSYSLEEADRALDDLRHGRFSGAAVLSVT"
     gene            complement(2506..>2971)
                     /locus_tag="VFQ35_10305"
     CDS             complement(2506..>2971)
                     /locus_tag="VFQ35_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931071.1"
                     /translation="VYTRNYAALVERKPEGIDEARTLLEGFLAAMQTELGVESERCVL
                     GGFSQGAMLATDTVLRSERRYGGLLILSGTLLTSTEWQALLPARRGLPVFQSHGREDP
                     ILPFELAERLQKLMTDAGLDVTWSPFSGGHGIPRPVLDRAGTVLSKVFRSAT"
BASE COUNT          442 a          992 c         1016 g          521 t
ORIGIN      
        1 cggcccagtg ggagatcacg tcgcgggtga tctgcagacc gaccttcacc gtgcgctcga
       61 ggctcacccg cgcgttcgtg ctgaggtgct tgctttcgct ctcgagcttc gccgacaaag
      121 catgcaggcg atgcgcgggg acgcgatccg ccgtttgctc ccagtgagcg agcagcgagg
      181 accccgccgg atcgggcgtg agcgccgctc gcacctcggc gtcgaagcgc gcgttcagcc
      241 gcctcgcaat cgactcgatc gcgagcagca tcgccggatg cttcttcatc cgcgtgacca
      301 ccgcgggcgt gtcctcgcgg taacggctcg ggagccgcgg gaacagcgcc tgacgtagcc
      361 ggctcgcgaa ctcctcgtac gtgcggatgc cgacgccgtg taccccgagc gctggcagca
      421 cgcccccgat gtagcgggcg agcgcatcgt tgaaaacgat gaccagcatc cgatcgggac
      481 gaaagcgccg cgaatcttga aaggcgaggt aggcgagccg atgaagaccg atggtcgtct
      541 tgccgctgcc cgcgccgcct tgaatcacga cgaggcccga gtcgggccgg gtgatgagct
      601 cgaactgacg ccggtcgatg agcggcgtga tctccttgag ctgcttctct tcgccgccct
      661 cttggccggt gccgagcttg ccgggacgat ggtgttgctc ggctcggatc gcgctgcccg
      721 cgccgcccgc gagccgcgtc gcggtgtcgc tcaggcgccg gaagctcccg tcgcggtttc
      781 tcgcgaagga gccctgggga gagccgacgc ggcgcagctc ggcgtcgctg atgaccacgc
      841 tgcgtcggac gctgaccgtg ccggagacct cgcgacctcc gaactcctcg tcgtacccgt
      901 cgccttcttc gtagcggtag aagagacggc tcaccggcgc gtcgcgccag tcgacgatgc
      961 ggatccccga acgcgtatcg aggtgggtgc cgcgcccgat cagcacttcg cggcggcggt
     1021 cgccctcctc caagatcaag cggccgaagt acggagagcg agcgtcgatc tctgtttcgg
     1081 tcacttggcg caggcggctc gcgaggctct gcaggcgctc catttcttga acgagcggtg
     1141 ggatgtcttc gagccgcgcc gaggcaattt ggtcgcgcag gtcgagcagg cgcgcgtcat
     1201 agtcgccgct ctcgtgcccg ctgcgagcgc gctccttctg tttgtgctcg ctgaggtgct
     1261 gcagcacctt cgtgaggcac tcttgttcct ccgtgacgat gcggtcacgt tcgggatctt
     1321 gctgcgcggg atcgattggg ggggcgggca tggccgtgcc ttggctaagg ggccggtctc
     1381 tccgggatcc gaagggaaat ttaaccagcc ggggcgacta gcacgaaccg gagcagccgg
     1441 catccggtaa gcgattggag gcgttcggaa cagaggctat cctgtctctc tcgttcgaag
     1501 agggaacacg aggcttggca cccacgatgc gagccatgtt gctcgaggca gcgcgttcac
     1561 cgctcgtcag gcggcaggtg cacgtgcccg agccgcgcgg cgatgaaatc ctgatcaagg
     1621 tccgctgctg cggcgtctgc cgcacggatc tgcacgtgct cgacggtgag ctgcccgggc
     1681 cgaagctgcc gctcatcttg gggcatcaaa tcgtcggcac catcgttgcg atggggcacg
     1741 aggtcgaggg cttcgtcgag agccagcggg taggcgtgcc gtggctcggg cgcgcgtgcg
     1801 gcgactgccg gtactgcacc tccggcaaag agaacctctg cgagcgggcg ctcttcaccg
     1861 ggtaccagct cgacggcggt tacgccgagt acgtggtcgc gcaagccaag ttcgcgttgc
     1921 ccctcccggg gtcgttcccg gatctccaag ccgcgccgct gctctgcgcg ggtttgatcg
     1981 gttatcgcgc gctcgtgatg agcggtgacg cagagtgcct gggcctctac gggttcgggt
     2041 cgtccgcgca catcgtcgcg caggtggcgc ggcaccaggg acgtcgcgtc tttgctttca
     2101 cgcggcccgg agactttgcc gctcaggatt ttgcccgccg gctcggcgcc gagtgggcgg
     2161 gcggctccga cgaggtgcct ccgtgcacgc tcgactcggc cataattttc gcgccggcag
     2221 gcacgctggt gccccgcgcg ctacgcgcga tttgccgcgg cggaaccgtc gtctgcgctg
     2281 gcattcacat gaccgacatc ccgtcgttcc cctacgagct cctttggggc gagcgaacca
     2341 tctgctccgt cgcgaacctc actcgacgcg acggcgtcga gttcttggag ctcgcgccac
     2401 gcgtgccgat ccggaccgag gtgcgctcgt attcgctcga agaagccgat cgagcgctcg
     2461 acgacttgag gcacgggcgc ttcagcggcg cggccgtgct cagcgtcacg tagcgcttcg
     2521 aaagactttc gagagcacgg tgccggctcg gtcgagcacc gggcgaggga tgccgtgccc
     2581 gccgctgaaa ggcgaccacg tcacgtcgag gcctgcgtcc gtcatcaact tctgcagccg
     2641 ctccgcgagc tcgaacggca ggatcggatc ttcgcggccg tggctctgaa agaccggcag
     2701 cccgcgccgc gcaggcaaca gcgcctgcca ttcggtcgac gtgagcaacg tgccgctcag
     2761 gatcaagagg cctccgtaac ggcgctcgct tcgcagcacc gtgtcggtag caagcatcgc
     2821 tccctgcgaa aagccgccga gcacgcagcg ctcgctctcg acgccgagct cggtctgcat
     2881 tgccgcgagg aagccctcga gcagtgtgcg cgcttcgtcg atgccctccg gcttgcgctc
     2941 caccaacgcg gcgtagttgc gcgtgtacac g
//
LOCUS       DASTKS010000495         4788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_301892, whole genome shotgun sequence.
ACCESSION   DASTKS010000495 DASTKS010000000
VERSION     DASTKS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301892"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1499
                     /locus_tag="VFQ35_10310"
     CDS             135..1499
                     /locus_tag="VFQ35_10310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931072.1"
                     /translation="MGPELGMVGVGETVAGKYRIERSIAVGGMGVVVAATHLSLGVPV
                     ALKFLTHEAKADGEAKARFAREAKAAAMIQSEHVCRVMDVGQLETGVPFIVMEYLEGR
                     DLSQLIASDGPLPQEKAVDYVLEAGEALAEAHALGIVHRDIKPSNLFLARQRDGSEVV
                     KILDFGISKLAPDSSGSDVRITSTRGLMGSPMYMAPEQIRASRDVDSRADVWAMGVVL
                     YELLTGGPAFEGATALELFAKIVVDAPTPLSTRRRVPSGLEEAIVKCLEKDRERRVAN
                     IQALAALLEPFASEEGRRSVARIQRTRAIKNASATVTSSVVSGSAGELATAAPTSAAW
                     GRTTPKPLRARRSLVIGGVVIAIALVALVLRATTAKAPSPTSPASVAASAPFAASAVP
                     TPRAAPVPVEVQVFPALTATSSRSASPTPTRSAKAKAIPSAAASRARPSASAKEEELF
                     GDRL"
     gene            1496..2524
                     /locus_tag="VFQ35_10315"
     CDS             1496..2524
                     /locus_tag="VFQ35_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931073.1"
                     /translation="MTNCFTSSSLRFASRALVFGAWLFSANGVRAAEPDSATAQALFS
                     SARKLANAGKFSEACAKFEESERIESAIGTEFHLAKCWEKLGRTASAWAMFLRVASRA
                     KAQNQSERAELASLRARALEGKLARLKIDVRAKTKGLEIFRDGEALGEASLGEAVPLD
                     PGEYEISARAPGYESFSTRVKLGESAGVSNVLIPELTPSVALPAEPPPPLHTSTPSKT
                     STSVNVAPSAAKVAPAGAETASSARPLAFVLGGLGIASVGVGVAFALDFKHVCPNREE
                     CQGRALSRAKTDRVLAYTGWGLGGAALATSLVLFLRSPARSDTRASWVLTPVCAPSTC
                     GATFTKEF"
     gene            2573..3907
                     /locus_tag="VFQ35_10320"
     CDS             2573..3907
                     /locus_tag="VFQ35_10320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931074.1"
                     /translation="MSFQVTSTQTENSGSSGSPGALALRWVYPNPRRAPLWVHGAHIV
                     GRDESADVILDSSQVSRRHAEVKQWGPVTLLTDLESKNGVQVNGVAIKTHALTAGDVV
                     RFGDVVAVVVEAPRNAELDFRSLAPGVFGGYRHRQAFALLERFAKSDLPIVLEGATGT
                     GKERFAQQAHALSGRPGDLVVVNCATYSTNLAAAELFGYRRGAFTGAEQSSLGHVRAA
                     EGGTLFLDELPELPAEVQPMLLRAIENQEVLPLGDSKTKKIDVRFVAATQTPLGKAVE
                     RGTFRADLRARLEVGLVRLPRLDECREIVPELFAKLYQGYGSEPPVLSAGFVEALCLL
                     ELSLNVRELDTLARRLAITPSGERLAAELLRNLPQRRPSSPPSPPDAGASEPMPRPSV
                     SVPGRPESPYSEEDVVRLKEALTRAGGNVSRAAAELGISRARAYRMLERGSR"
     gene            complement(4214..>4788)
                     /locus_tag="VFQ35_10325"
     CDS             complement(4214..>4788)
                     /locus_tag="VFQ35_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931075.1"
                     /translation="WESPAVPAFEEPPSPVPPEPDAPTVPPPLLAKLPAVPAAPPLPA
                     PPAAEPPLPAPPAALDAPPLPPELALVPPVGTDLPPEPLAPPLETPPVSVAIAPDAPP
                     SPEPSAPPIIGSAPPLSFATSPPLEEEPPRVDDVAPPLSDFAPPCAPPPGVEAPAEPD
                     APAAPPEPAGPSGKVKQLVTPSNKATSSRA"
BASE COUNT          797 a         1501 c         1683 g          807 t
ORIGIN      
        1 tgccaactcg gtgacaccga cgcattcagt gttcggctct cttgatcatg ctttcagcag
       61 agccggagtc gaacgaaccg actgcacgcc acgatcgaag aatcagcttg gcgcagggag
      121 atggagagag gaagatgggc ccggagctcg ggatggtggg tgtgggcgag accgttgcgg
      181 gaaagtaccg catcgagcgc agtatcgcgg ttggcggcat gggtgtcgtc gtcgccgcga
      241 cacacctttc gctcggggtg cccgtggcgc tcaagttcct cactcacgag gcgaaggcag
      301 atggggaagc gaaagctcgg ttcgcgcgcg aagccaaagc cgcggccatg atccagagcg
      361 agcacgtgtg tcgcgtgatg gacgttgggc agttggaaac gggcgtgccg ttcatcgtga
      421 tggagtacct cgaggggcga gatctctccc agctcatcgc aagtgacggg cctctgcctc
      481 aggaaaaggc cgtcgactac gtgctcgagg ccggtgaagc gttggcggag gcgcacgcgc
      541 tcgggatcgt tcaccgagac atcaagcctt cgaacctgtt tctggcgcgg caacgcgacg
      601 gttccgaggt cgtcaagatt ctggatttcg gtatctcgaa gctcgcgccc gactcgagcg
      661 gcagtgacgt gcgcatcacg tccacgcgag gcctgatggg ttcgcccatg tacatggcgc
      721 cagaacagat tcgtgcatcg agagacgtcg actcgcgagc ggatgtctgg gcgatgggtg
      781 tcgtgctgta cgagctcttg acgggagggc ccgcgttcga aggggcgact gctctcgaac
      841 tgttcgccaa gatcgtggtg gacgcgccga cgccgttgag tacgcgccgc cgggtgccta
      901 gcgggctcga ggaggcgatc gtcaagtgcc tggaaaaaga ccgagaacga cgggtcgcga
      961 acattcaagc gctcgccgcg ttgctagagc cgttcgcgag tgaagaagga cggcgttcgg
     1021 tcgcgcgcat ccaacgcacg cgcgccatca aaaacgcgag cgctacggtc acgtcgtccg
     1081 tggtgagcgg ttcggcgggc gaacttgcga ccgcggctcc cacttcggcc gcgtggggtc
     1141 gcacgactcc caagccgctc cgcgcgcgac gctccctcgt cattggcgga gtcgtcatcg
     1201 cgattgcgct ggtcgcgctg gtgttgcgcg caacgacggc gaaggctccc tcgccgacct
     1261 ccccggcttc ggtcgcggct tcggctccat tcgcagcgag cgccgtcccc acgccccgcg
     1321 ccgccccggt ccccgtcgag gtccaggtct tcccggcgct cacggcgacg agttcgcgct
     1381 ccgcgtcgcc gacaccaacg cgcagcgcca aggcgaaagc gattccgagc gccgccgcga
     1441 gccgcgcccg tccgagcgcg tccgccaagg aagaagagtt gttcggtgac agactatgac
     1501 gaactgtttc acgtcttcca gccttcgctt cgcttcgcgc gcgctcgtct ttggcgcgtg
     1561 gctcttcagc gcaaacgggg tgcgcgcagc cgagcccgat agcgcgacgg cgcaagcgct
     1621 gttctcgtcg gcgcgaaagc tcgccaacgc tggaaagttc agcgaagctt gcgcgaagtt
     1681 cgaagagagc gagcgcatcg agtcagcgat tggcaccgag tttcatctcg ccaagtgctg
     1741 ggaaaagctc ggtcgcaccg cgagcgcgtg ggcaatgttc ttgcgcgtcg cttcacgagc
     1801 gaaggcgcaa aaccagagcg aacgcgcgga gctcgcgagt ctccgagcac gcgccctcga
     1861 aggcaagctc gcgcgcctga agatcgacgt gcgcgcaaaa acgaaggggc tcgagatttt
     1921 tcgcgatgga gaagccctcg gtgaagcttc gttgggcgaa gccgtgccgc tcgatccagg
     1981 cgaatacgag atttcggcgc gcgcgccggg ttacgagtcg ttctcgaccc gagtgaagct
     2041 cggcgagtcg gcgggtgtgt cgaacgtact gatcccggag ctcactccga gcgtcgcgct
     2101 gccagccgag ccgccgcccc cgttgcacac ctccacccct tcgaagacga gcacgagcgt
     2161 gaacgtggcg cccagcgcgg cgaaggttgc gccagccgga gccgagactg cctcgtcggc
     2221 tcgaccgctc gcgttcgtgc tgggcggttt ggggattgcc agcgtcggcg tgggcgtcgc
     2281 gtttgcgctc gatttcaagc acgtgtgtcc gaatcgcgag gagtgccaag ggcgagcgtt
     2341 gagtcgcgcc aaaacggatc gggtgctcgc ctacacaggt tggggtctgg gcggcgccgc
     2401 gctcgcgacg agcctcgttc tgttcctacg gtcgcccgcg cgcagcgata cccgcgcgag
     2461 ttgggtgctg acgccggtgt gcgctccttc cacctgtggc gcgacgttca ccaaggaatt
     2521 ctgagtacga cgagcaagct cgtttcggca actctgcagc attgagaaac cgatgtcttt
     2581 ccaggtcacc agcactcaaa cagagaactc gggtagtagc ggttcccccg gcgcgctcgc
     2641 gctgcgctgg gtttatccca atccgcgcag ggcaccgctt tgggtgcacg gggcgcacat
     2701 cgtcggtcgc gacgaatccg cggacgtcat cctcgacagc agccaggtgt cgcgccgcca
     2761 cgccgaggtg aagcaatggg gtccggtgac gctcctcacc gacctcgaga gtaagaacgg
     2821 agttcaggtg aacggcgtcg cgatcaagac gcacgcgctc accgccggcg atgtcgtgcg
     2881 ctttggagac gtggtcgccg tcgtcgtcga ggcacctcga aatgcggagc tagactttcg
     2941 ttccctcgcg ccgggcgttt tcggcggcta ccgtcatcgt caggcgttcg cattgctcga
     3001 gcgatttgcc aaaagcgatc tgcccatcgt gctcgagggc gccaccggga cgggcaagga
     3061 gcgcttcgct cagcaagcgc acgctctcag cgggcgccct ggcgacctcg tggtcgtcaa
     3121 ttgcgccacc tactcgacga acctcgccgc tgcagagctc tttggctacc gccgtggcgc
     3181 tttcaccggt gcggagcagt ccagcctcgg tcacgtgcgc gcggccgagg gtggcacgct
     3241 gtttctggat gagctccccg agctgcccgc agaagtgcaa ccgatgctgt tgcgcgccat
     3301 cgagaaccaa gaggtgctgc cgctcggtga ctccaagacc aaaaagatcg acgtgcggtt
     3361 cgtcgctgcg acccaaactc cactcggaaa agcggtcgag cgaggcacgt ttcgcgctga
     3421 cttgcgcgcg cgactcgagg tgggcttggt gcgcttgccg cgcctcgacg agtgccgcga
     3481 aatcgtgccc gagctcttcg caaagctcta tcaggggtac ggctcggagc cgccggtcct
     3541 gagcgccggc ttcgtggaag cgctgtgtct gctcgagctg tcgctcaacg tgcgtgagct
     3601 cgatacgctg gcgcggcgcc tcgcgattac gccttcgggc gagcgcctgg cggctgaact
     3661 gctcaggaac ttgccgcagc gtcggccttc ttcgccgccg tcgccgccag atgctggcgc
     3721 ctcggagccc atgccgcggc cgagcgtgtc ggttccgggc cgccccgaga gcccatactc
     3781 ggaggaggac gtcgtgcgtc tcaaggaggc gttgacgcgc gcagggggca acgtgagtcg
     3841 cgcggcggca gagctcggca ttagccgtgc gcgggcgtat cgaatgttgg agcgcggcag
     3901 ccgatgagcg gcaacatgtc cgcgagtgtt cgcgaacgtt cgcgagacga gagtgcgagc
     3961 gctcgtcctc tcgtctcaca tcgcgagtcg aaggctcgtc gttcgcggcc cggtaggctc
     4021 gttcgccgtg caaaggttgg gaaaaccccg gtgcagcgga gttcacgcac gagcgctctc
     4081 gagtgcgggt acttggcaca tgggttgctc tcgctcgagt gcgcatgagg caaccgtatt
     4141 ggcacgagat cttcatgaac cgaaacagta aaaccagcgc gaccaaacca attacgacga
     4201 ggccgcgggc gcgtcatgca cggctcgaag tcgccttgtt actgggagtg actagctgtt
     4261 tgaccttgcc ggacggcccc gcgggctcgg gcggcgctgc gggggcgtcg ggctcggcag
     4321 gcgcttcgac tcctggcggt ggcgcgcatg gaggagcgaa gtcggagagc ggcggtgcga
     4381 cgtcatcgac gcgcggcggt tcctcttcga gcggcggcga tgttgcgaac gagagcggcg
     4441 gcgcgctgcc aatgatgggc ggcgcggacg gctcagggga aggcggagcg tccggcgcaa
     4501 tcgcgacaga cactggtggc gtttcgagcg gcggcgccaa cggctcgggc ggcaggtcgg
     4561 tgcccacggg aggcacgagc gccaattcgg gcggcaaagg aggcgcgtcg agcgcggctg
     4621 gcggcgccgg caatggcggc tctgcggcgg gcggcgcggg cagcggtggc gcggctggca
     4681 ccgcaggcag cttcgcgagc agcgggggcg gcacggtggg cgcatcgggc tccggcggca
     4741 ccgggctcgg cggttcttcg aacgcgggca ctgcgggtga ctcccaat
//
LOCUS       DASTKS010000496         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_526132, whole genome shotgun sequence.
ACCESSION   DASTKS010000496 DASTKS010000000
VERSION     DASTKS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526132"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFQ35_10330"
     CDS             complement(<1..195)
                     /locus_tag="VFQ35_10330"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HET9931076.1"
                     /translation="MIQFKLRLYITGRTGQSQRALANLRAICDSALPDAYEVEVIDVL
                     ENPALAESEKILATPTLVKWL"
     gene            complement(192..1025)
                     /locus_tag="VFQ35_10335"
     CDS             complement(192..1025)
                     /locus_tag="VFQ35_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931077.1"
                     /translation="MNPAAVRERVLLLIEDNPGDADVVRHELAQTQPEAYRVLHVTCL
                     SAARRCLESNSTDLLLVDLMLPDSSGVDTVRQVREFAHGLPIVVLTSADDELALECIN
                     AGAQDFLPKSELRTRNLRRALGYALTRAREAQLQELQRLTERYRTLSSAGQGTVVTAA
                     LAGSGAVSARYPDVFAELVQDYAELLAPCLVRGADAFAPPRHEMERIVTSLGDMSGGP
                     RDLIDVHVGALERLEKFPNGSPARASVLEARLIALEMMGLLVDYYRVGYRRRSAKGAG
                     Q"
     gene            1581..>2352
                     /locus_tag="VFQ35_10340"
     CDS             1581..>2352
                     /locus_tag="VFQ35_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HET9931078.1"
                     /translation="MLASNTLKSGWSLSLFVSGRSGTSLRALRNLTALCHGALAGDRA
                     VTIVDVHEQPDLAESQRILWTPTLVRNEPAPTRRIVGDLSNRERLFEALDLSERPVRT
                     STASPPSKATSPVVSLQTRVAASPATRDAQREIVSDEAFRTMVMRSPGGVLLLDGHGH
                     VLFGNPAAERLFADSPAPLLGRFLGIPWMEGESPLRLRVTGDRVVDAHSTAIDWRGRL
                     LQMVLLHDVTAYAVAERQGAELVSELLAVTQKLGRDANV"
BASE COUNT          420 a          759 c          760 g          413 t
ORIGIN      
        1 gagccatttg accagagtag gcgtggccaa gatcttctcg ctttcggcga gcgcggggtt
       61 ctccagcacg tcgattacct cgacttcgta ggcgtccggc agcgctgaat cacagatggc
      121 ccgtagattc gcaagcgctc gctgcgactg gcccgtgcga ccagtgatat agagccgcaa
      181 cttgaattgg atcattgccc ggctcctttt gcggaacgcc gccggtatcc aacgcggtag
      241 tagtcgacta gcaagcccat catctccaag gcgatgagcc tcgcctcgag cacgctcgcg
      301 cgggccggcg acccgttggg gaatttctcg agccgttcga gcgcgccgac gtgcacatcg
      361 atgaggtcac gcggcccgcc gctcatgtct ccgagagacg taacgatgcg ctccatttcg
      421 tgccgggggg gagcgaacgc gtcggcccca cgaaccaggc acggagccaa gagctcggcg
      481 tagtcttgca ccagctcggc aaagacgtcc gggtagcgcg ccgacacggc gccggaaccc
      541 gcgagcgccg ccgtcactac ggttccctgg ccagccgacg acagcgtgcg gtatcgctcg
      601 gtcaaccgtt ggagttcttg cagctgcgcc tcgcgagcgc gggtcagcgc atagccgagc
      661 gcgcgacgga gattccgcgt ccgcagctcg gatttgggca gaaagtcctg ggcgccggcg
      721 ttgatgcact cgagcgcgag ctcgtcgtcc gccgacgtca agaccacgat cggaaggccg
      781 tgcgcaaatt cgcgaacctg tcgaaccgta tctactccgc tgctgtcagg aagcatcaga
      841 tcgactagca ggaggtcggt cgagttggat tcgaggcatc gacgcgcggc cgaaaggcaa
      901 gtcacgtgca gaacccgata tgcttcaggc tgcgtctgcg cgagctcgtg ccgtacgacg
      961 tcggcgtcac cggggttgtc ctcgatgagc aacaagacgc gctcgcggac ggcagcgggg
     1021 ttcatgccgc ctcctcagcc acaggctcga gctcggcgct ggagccgcat actgctcgcg
     1081 tccagcaaag cccgtatcga agcctcatgg cgcaactatc gacatccccc agggcagtcg
     1141 atagtcgcga gatttctcgg gtattgtcag agagttgctg acatgcggcc gcgcgcgaca
     1201 cgcactcggc tggcgcaggg cggacgggta cgccgactcc tcggaggcaa cgtcagcaaa
     1261 actcttacaa gcctcggcgc aaacgctcgc gctcgctggg cttcgaggtc ccgaaacggg
     1321 ctcgagcggg tgacgttgcg tgaggagacg caagctctcc caggcgaacg agaaaggcgc
     1381 tgtctgttgg gttcgagcgc cttgccgcct cttggcaggt gacgacgggg gcctctgctc
     1441 gcgcagaccc agcgagtttc gccgagcctc ggcgtcgtat tcttgacgcg gctcgctccc
     1501 gcccgcgcat cttcgctctt ggaatacgtc gcgcgtcgtg ggtacatgtt cggttgcacg
     1561 agatactgga gtaacggtgc atgctggcga gcaacaccct gaagagcggt tggtctctgt
     1621 cattgttcgt atcgggtagg tcgggcactt cactgcgcgc gctgcgcaac ttgactgcgc
     1681 tatgtcatgg ggccctcgcg ggagaccgcg ccgtgaccat cgtcgacgtg cacgaacagc
     1741 cggacttggc cgagtcccaa cgcatcctgt ggacgccaac tctggttagg aacgagccgg
     1801 cgccaacgag gcggatcgtg ggagatctct cgaatcgcga acgcctattc gaagcgctcg
     1861 atctctcgga gcgacccgtt cgcaccagca ccgcctcacc cccgagtaag gcaacgtcgc
     1921 ccgtggtttc gctgcaaacg cgagtagccg cgtctccggc cacgcgcgac gcccagcgcg
     1981 aaatcgtcag cgatgaagcg tttcgcacca tggtaatgag gagccccggc ggagtcctgt
     2041 tgctcgatgg ccacggccac gtgctcttcg gaaacccggc cgccgagcga ctgttcgccg
     2101 attcacccgc cccgctgctc ggacgcttct tggggatccc ctggatggaa ggggagagcc
     2161 cgctgcgact gcgcgtcacg ggcgaccgcg tggtcgacgc gcactcgact gcaatcgact
     2221 ggcgaggccg actcttgcag atggtcctcc tccacgacgt caccgcttac gccgttgccg
     2281 agcgtcaggg agcagagctg gtttccgagt tgctggccgt cacgcaaaag ctaggtcgag
     2341 acgcgaacgt gg
//
LOCUS       DASTKS010000497         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_971759, whole genome shotgun sequence.
ACCESSION   DASTKS010000497 DASTKS010000000
VERSION     DASTKS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971759"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFQ35_10345"
     CDS             complement(<1..379)
                     /locus_tag="VFQ35_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931079.1"
                     /translation="MVSVDDELDANGSRLASTIVGAAYRRIDPGHVAATATTLCRRIE
                     ERFPRSGLLRVAHELEAVSREARALASDVAHTPATVRWGVRACIGLLLCVPLALATQI
                     KFGGDAPGWSDFLQGLEALVNDVV"
     gene            complement(387..1157)
                     /locus_tag="VFQ35_10350"
     CDS             complement(387..1157)
                     /locus_tag="VFQ35_10350"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9931080.1"
                     /translation="MKTIRLFQPLPSRSARIFRALTDPQDLAAWHADVVRGRVEAGRT
                     LELAWPRLGASLELSVERVVPGRRIVLSSALGNLEMSVENGGVELCHSAAFDEDTHAG
                     MESSWRVTLSILNTYLGRHVDRARQVHWSMARVHASPELCHAYFTDPHLLRTWFGAAD
                     DGLGGVESLVSLTLGPKLRVSGRVVAHTEGRDVALRWQDADDSVLVFRTLPVGDGSRC
                     ALLGWSRWSEPPNTEEITRALDQAAQRLERKLEGRARA"
     gene            1248..2456
                     /locus_tag="VFQ35_10355"
     CDS             1248..2456
                     /locus_tag="VFQ35_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HET9931081.1"
                     /translation="MALAKNHAKLFWLSCGVALVTGCSAPDSTEENGSLESPISGGTT
                     DAVNTNVFGLLAHADDGVGACSSTLIAPNLLLTARHCIAHTTDEQVICGKATFSEPYP
                     ASAVIASNALDLNRTKDWYHASEIFVPSDGNDTCGYDVALIVLRETVAASVATPAVPR
                     IDREVARGESYHAVGYGLTETMDYGVRRMLTEREVQCDPGTCRSGVRGNEFVGTAGPC
                     QGDSGGPAFDTDGKVVGVDSRGTEGCEEPVYSTVTGWKDFIITTALHAAEVGAYKPAS
                     WVTTGSSDPPVQPPPPPPPPVALGDTCDAASVCADGGACYQPGTGGAARCVAVCKESS
                     ECRQGDECVPLSGQVAVCLEAPPSEASSGCQMGRTPSSALAVAVGGLALAFVSRRRRV
                     RAQPADNKSR"
     gene            complement(2429..2872)
                     /locus_tag="VFQ35_10360"
     CDS             complement(2429..2872)
                     /locus_tag="VFQ35_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931082.1"
                     /translation="MRTMSLFERDLLRRLIQDFARMLAAIVGMRQRGELDDAESELAS
                     YVNALLGQLAKDAEQLDSETLGKLLKVERLRHYALLIAERANLRAARGAHSEALEDRS
                     RALELLLEARAKSGDTSDAQLIRELASEISLAGLAPRYRDLLSAG"
BASE COUNT          523 a         1007 c         1024 g          507 t
ORIGIN      
        1 agacgacgtc gttgaccagc gcctccagcc cttgaagaaa atccgaccac ccgggcgcgt
       61 cgccgccgaa cttgatttgc gttgccagcg cgagcggcac gcacaagagc aggccgatgc
      121 acgcgcgtac accccaccgc acggtggccg gcgtatgggc gacgtcgctc gccaacgccc
      181 tcgcctcgcg cgaaacggcc tcgagctcgt gtgcgactcg caaaaggccg gagcgaggaa
      241 agcgctcttc gatgcggcgg caaagcgtcg tcgccgtcgc ggcgacgtgg cccggatcga
      301 tacggcgata cgcggcgccg acgatggtgg aagcaaggcg gctcccgttc gcgtcgagct
      361 cgtcgtctac gctgaccacg tcccgctcac gcgcgcgcgc ggccctcgag tttgcgctcg
      421 aggcgctgcg cggcttggtc cagagcgcgc gtgatctctt ccgtattcgg cggctcgctc
      481 cagcgcgacc aaccgaggag cgcgcagcgg ctgccatcgc ccacgggcag agtgcgaaac
      541 acgagcacgg aatcgtcggc gtcttgccag cgcagagcga cgtcgcggcc ttcggtgtgc
      601 gcaacgacgc gacccgaaac acgaagcttg ggacccagcg tgaggctcac tagggactcc
      661 acgccgccga ggccgtcgtc ggccgcgcca aaccaggtgc gtaagaggtg agggtcagtg
      721 aaataggcgt gacagagctc tgggctcgcg tgcacgcgcg ccatcgacca gtgtacttgt
      781 cgtgcgcgat cgacgtggcg tccgagatac gtgttcaaga tggagagtgt gacgcgccat
      841 gacgattcca tcccagcgtg cgtgtcttcg tcgaaggcgg cgctgtggca gagctcgact
      901 ccaccgttct ccaccgacat ctcgagattt ccgagcgcgc tcgagagcac gatgcgacgg
      961 ccgggcacca cccgctccac cgagagttcg agcgacgcac cgaggcgtgg ccatgcgagt
     1021 tcgagcgtgc gcccggcttc cactcggccc cgcacgacgt cggcatgcca agcggcgaga
     1081 tcctgcggat ccgtaagcgc tcgaaaaatt ctggctgagc ggctggggag cggctggaaa
     1141 agtcgaatgg ttttcacggg atcaggtctc gaatcgagga aacttcaggg aatggagggg
     1201 gggcgcccga tgtgcgctcg aaaacagctt acacttttgc tccttgcatg gcgttggcga
     1261 agaatcacgc gaaactgttc tggctctcgt gtggggtagc gctggttacg ggctgtagcg
     1321 ctcccgactc gacggaggag aacggctcgc tcgagtcgcc gatctccggt gggaccacgg
     1381 acgccgtgaa caccaacgtt ttcggcctgt tggcgcacgc cgacgacggt gttggcgctt
     1441 gctcgtcgac cctgatcgcg ccgaacctgc tcctcaccgc gcggcattgc attgctcaca
     1501 cgacggacga gcaggtcatc tgcggcaagg ccactttctc ggagccttac ccggcgagcg
     1561 ccgtcatcgc gtcgaacgcg ctcgacctca accgcaccaa ggactggtac cacgcgagcg
     1621 agatcttcgt gccttccgat ggcaacgaca cgtgcgggta cgacgtggcc ctcatcgtgc
     1681 ttcgggagac cgtcgccgcg agcgtggcga ctccggcggt tccgcgtatc gatcgcgagg
     1741 tcgcccgcgg cgaaagctac cacgcagtgg gatacgggct caccgagacg atggactacg
     1801 gcgtgcgtcg catgctcacc gagcgcgaag tccagtgtga tccgggcacg tgccgctcgg
     1861 gcgtgcgagg aaacgaattc gttggcaccg cggggccgtg ccaaggcgac tcgggtggcc
     1921 cagcgttcga cacggacggc aaggtcgtcg gcgtcgattc gcgcggcacc gaaggctgcg
     1981 aagagccggt gtacagcacg gtgacaggct ggaaagactt catcatcacg actgccctcc
     2041 acgctgccga agtcggcgcg tacaaaccgg cctcgtgggt gacgactgga tccagcgatc
     2101 caccagtcca gcccccgcct ccgccgcctc ctcctgtcgc gttgggcgac acttgtgacg
     2161 ccgcgagcgt gtgcgccgat ggcggtgctt gctatcaacc ggggaccggt ggcgccgcgc
     2221 gctgtgtggc cgtgtgcaaa gagagctccg aatgccgcca gggtgacgag tgcgtgccgc
     2281 tgtcgggtca ggtcgccgtg tgcctggaag ctcctccgag cgaagcttct tcgggctgtc
     2341 agatgggacg aacgccgtcg tcggcattgg ccgtcgcggt cgggggactc gcgttggcgt
     2401 tcgtctcgcg cagacgacgc gttcgcgctc agccagccga caacaaatcg cggtagcgcg
     2461 gagccaaccc agcgagcgag atctcgctcg cgagctcgcg gatgagctgc gcatcgctcg
     2521 tgtcgcccga cttcgcacgc gcctcgagga gcagctcgag agctcgcgag cggtcctcga
     2581 gcgcttccga gtgcgcgccg cgcgccgccc gcaaattcgc gcgctccgcg atgaggagcg
     2641 cgtagtggcg aagtcgctcg accttcaaca actttccgag cgtctcggag tccagttgtt
     2701 cggcgtcctt cgcgagctga ccgagcaggg cgttcacgta ggacgcaagc tcgctctccg
     2761 cgtcatcgag ctcaccccgc tgtcgcatgc cgacgatcgc tgcgagcatg cgcgcaaagt
     2821 cctgaatcag ccgtcgaagg agatcgcgct cgaacaggga catggtccgc atcgttggcg
     2881 cgtaccagac ctccgtcaag ccgcggtcac gcccgactca gttcacttga cgagctctgt
     2941 atcgctggcg atctctcgta cgcgcgcggc gtgcacgtcc tttgggtagc gctccaggta
     3001 ttgcagcgcg agcctgcgag cgccgttcct atcgttgcgc gcgaccaggg cctcgatgcg
     3061 a
//
LOCUS       DASTKS010000498         4998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_971845, whole genome shotgun sequence.
ACCESSION   DASTKS010000498 DASTKS010000000
VERSION     DASTKS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971845"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFQ35_10365"
     CDS             complement(<1..557)
                     /locus_tag="VFQ35_10365"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET9931083.1"
                     /translation="MTPQIPHVPLPELVLVPAGAFGNSTPFSLKPFGLLVATGVYLGA
                     FMTTRYARKRGVQPAAIMSFVYWVVGIGFVGGHMLDVLFYSPERLSSDPFALFKLWDG
                     LSSFGGFIGGTVGALLWRLKHRVSILPYADLVASGLPLGWVFGRAGCAVVHDHPGIAS
                     DAWFAVSYPGGGRLDLGLIEMALTIP"
     gene            complement(554..1516)
                     /locus_tag="VFQ35_10370"
     CDS             complement(554..1516)
                     /locus_tag="VFQ35_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931084.1"
                     /translation="MQLLSGGEQVTRWLPVALLLLGCSVGNRVAAPRADYALFRQTRV
                     ANSPAERLGAGSRYLRELPEGRFRAEVRAWFEVAEPRFVAEAHDRPSMLRAYLRELPN
                     GPHAQQVRDRLEEFALMNEYRGRKAAERERFVQRVEAQFEAAKVGREHLIESVATLIS
                     AMAKTQTLGQPPSALDSALAPASETCSSELCERVFDIEYSVPTRTGLVARSARLTLAI
                     ELTGGVVKRIRLAGPELFTRIGEAVDRVAIEPDNLLGRTEAIARTQQVVENAIESALP
                     SGECRRDVVAPVVLSRSCRGVSLSLVVATQLGELDRAELTAQPK"
     gene            complement(1486..1893)
                     /locus_tag="VFQ35_10375"
     CDS             complement(1486..1893)
                     /locus_tag="VFQ35_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931085.1"
                     /translation="MKKALVWARGLAFSRAARAAFAFVVLAFCAAPVPGDVGGCNQSA
                     EELDPVAFFRTKARIDCDQCTQCGISTAACSQACQGSPQTSFPKDCLPLAHDGEVCLR
                     ALLDAGCGDYAEYVRDQSPTVPTECNFCPEVSK"
     gene            complement(1890..2510)
                     /locus_tag="VFQ35_10380"
     CDS             complement(1890..2510)
                     /locus_tag="VFQ35_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931086.1"
                     /translation="MRSLSVLGAVAVTLGLARPCCAEAPAASAPKEPAPVEVEARRPY
                     VHLFSSFGYGRGVRWNNPYRLQTELGSGADSASLTAGYFDAGLGAMLGDPNGLQHGAV
                     LHLSFAANGISQQVLSLGYVGALPLGASALAYARAGVPFVLSPDTGIGFEVAAGATYF
                     VTGALGVNAELVQSLFVGAATWEHDPSLYPVTSLQLGLWFDYEVLP"
     gene            complement(2498..3799)
                     /locus_tag="VFQ35_10385"
     CDS             complement(2498..3799)
                     /locus_tag="VFQ35_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET9931087.1"
                     /translation="MTLQDEFRTRQSRVLEAIHPGALLLFAAPVAIRNNDVEHEYRQD
                     SDFFYLTGFDEPESVLLLRSDAEAKATLFVRPRDPERETWDGPRAGIEGAVRDFGADQ
                     AFPIAELDAKLPELLRGQPRLFYRLGQTEDEDARVLKAIASLRRRTRQAMVYPTSIVE
                     PDGLLHEMRLRKSAAEVELMRRAVDITNEGHRELMRITRPGMYEFELEGLLRRCFRAA
                     GSERPAYSPIVGSGPNATILHHRRNDRRIEEGDLVLVDAGCEYGYYAADVTRTYPASG
                     RFSPAQAKIYELVLQAQLAAIEKSTVGSTLEAVHEAALQVVTEGLIALGLIEGPLDVA
                     IKEQRYKPYFMHRTSHWLGMDVHDVGRYYEGEQPRPLAPGMVLTVEPGIYVSQSANVS
                     PEYQGIGVRIEDDVLVDAAGPVVLSHQIPKTIEEVEAACAR"
     gene            3918..4409
                     /locus_tag="VFQ35_10390"
     CDS             3918..4409
                     /locus_tag="VFQ35_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931088.1"
                     /translation="MATERPPEGDDREREQRRLERLIPELLRRVLEVGYEKLTEGPES
                     VRNLVSELKLPKEALGVLISQLDETKNGLYRAVAKEVRDFLEQTNFAEELAKVLTTLS
                     FEVKTEVRFIPNDQRLRATPDIRTKVSVKRDRASQPPPEPKAPSSDTTESRPPNDSEE
                     KTE"
     gene            4406..4888
                     /locus_tag="VFQ35_10395"
     CDS             4406..4888
                     /locus_tag="VFQ35_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931089.1"
                     /translation="MKPLTAGSEIDAWCTKCRMDLGHRVVAMVAGAPKRVVCLTCGAE
                     HNYRAPRNEKQSSGSSVHLRSNRESARPPAAPKASGGVRAAAKAKNEAERYEGWASKT
                     LGQAVDAFTRYSMERTFRVGELVMHPKFGEGYVDQVLEGGKVNVMFREGPKVLAHRVS
                     "
BASE COUNT          924 a         1662 c         1648 g          764 t
ORIGIN      
        1 gggatcgtga gcgccatttc gatgaggccc agatcgagcc gcccgccgcc gggatacgaa
       61 accgcgaacc aggcgtcgct cgcaatgccc gggtggtcgt gcacgaccgc gcagcccgcc
      121 cgaccaaaca cccacccgag agggagaccg ctggcgacga ggtccgcgta cggaaggatc
      181 gaaacgcgat gcttcaagcg ccaaagcagc gcgccgaccg ttccgccgat gaaaccgccg
      241 aagctcgaaa gcccatccca caatttgaag agtgcaaacg gatccgagct cagccgctcc
      301 ggtgagtaga agagcacgtc gagcatgtgg ccgccgacga acccgatgcc gacgacccag
      361 tagacgaagg acatgatcgc cgccggctga acaccgcgct tgcgcgcgta acgggtcgtc
      421 atgaacgctc ccaggtagac gccggttgcg acgagcaggc cgaacggctt caagctgaag
      481 ggcgtcgaat tgccgaaggc gccggcggga accaggacga gctcgggcaa cgggacgtgc
      541 gggatctgcg gggtcatttg ggttgggcgg tcagctcggc tcggtcgagc tcgccgagct
      601 gagtggccac cacgaggctg agcgagacgc cgcgacagct tcgcgacagc acgacgggcg
      661 ctaccacatc ccggcggcac tcgccgctcg gtagcgcgga ttcgatggcg ttttcgacca
      721 cttgttgcgt tcgcgcgatc gcctcggtgc gaccgaggag attgtcgggc tcgatcgcga
      781 cccgatcgac cgcttcaccg atgcgagtga agagctccgg cccagccaaa cgaattcgct
      841 tcaccacgcc gcccgtgagc tcgatcgcga gcgtgagccg agcgctgcgc gcaacgagcc
      901 cggtccgagt cggcaccgaa tactcgatat cgaatacgcg ctcgcagagc tccgacgagc
      961 aagtctccga ggcaggcgcg agtgccgaat cgagcgccga gggaggctgc ccgagggtct
     1021 gcgtcttcgc cattgccgaa atcaacgtcg cgacgctctc gataaggtgc tctcgcccga
     1081 ccttcgccgc ctcgaactgg gcctcgacgc gctgcacgaa gcgctcgcgc tcggcggctt
     1141 tgcgtccgcg atactcgttc atgagcgcga actcttcgag ccgatcgcgg acctgctgcg
     1201 cgtgggggcc gttcggcagc tcacgcagat aggcgcgcag catgctgggc cggtcgtgcg
     1261 cttccgcgac gaagcgcggc tcggcgacct cgaaccaggc gcgcacctcc gcgcgaaagc
     1321 gcccctcggg tagctcgcgc aagtagcggc tgcccgcgcc gagccgctct gcagggctat
     1381 tcgcgacgcg cgtctgccgg aacagggcgt agtcggctcg cggtgcggcg acgcggttgc
     1441 cgacgctgca gcccaacaag aggagcgcga cggggagcca gcgcgtcact tgctcacctc
     1501 cggacagaag ttgcattcgg tcgggaccgt cggcgactgg tcccgcacgt attctgcgta
     1561 gtcgccgcag cccgcgtcga gcagcgcgcg caagcagact tcgccgtcgt gcgcgagcgg
     1621 aaggcaatct ttggggaagc tcgtttgcgg cgacccttgg caagcctgcg agcaggccgc
     1681 cgtggagata ccgcactggg tgcactggtc gcagtcgatg cgagctttgg tacggaagaa
     1741 cgcgaccggg tcgagctcct cggcgctctg attgcagccg ccgacgtcac ccgggaccgg
     1801 cgccgcacaa aacgcgagga cgacgaacgc gaacgccgcg cgcgcggcgc ggctaaaggc
     1861 gagaccgcga gcccaaacca gcgccttttt cacggcaaca cctcgtagtc gaaccacaag
     1921 ccgagctgca gcgaagtcac cggatacagg ctcggatcgt gctcccacgt cgccgccccg
     1981 acgaacagac tctgcacgag ttcggcgttc acaccgagcg ctccggtgac gaagtaggtc
     2041 gccccggccg cgacctcgaa gccgatccca gtatcggggg acaacacgaa cggcaccccc
     2101 gcccgcgcat aggccagcgc gctcgcgccg agcggcagcg cgccgacata gccgaggctc
     2161 agcacctgtt gcgaaatccc attggcagca aatgacaagt gcaggacggc accgtgctgc
     2221 aagccgttcg gatcgccgag catggccccg agcccagcgt cgaagtatcc ggcggtgagc
     2281 gacgcgctgt cggcaccact gccgagctcg gtttgcaacc gatacgggtt gttccaacgc
     2341 acgccgcgcc cgtaaccgaa cgacgagaac aagtgtacgt acggacggcg tgcctcgacc
     2401 tcgacgggcg ctggttcttt cggcgcggac gctgctgggg cctcggcgca gcacggccgc
     2461 gcgaggccca gcgttacggc gaccgcgcca agaacactca acgagcgcac gcggcttcga
     2521 cttcctcgat ggtcttcggg atctggtggc tcagcaccac cggtccggct gcgtcgacga
     2581 gcacgtcgtc ctcgatgcgc acgccgatgc cttggtactc gggagagacg ttcgcgcttt
     2641 gcgagacgta gatccccggc tcgaccgtca ggaccattcc cggcgcgagc ggccgcggct
     2701 gctccccttc gtagtagcga cccacgtcgt gcacgtccat cccgagccaa tggctcgtgc
     2761 gatgcatgaa gtacggtttg tagcgctgct ctttgatggc gacgtcgagc ggtccctcga
     2821 tgagcccgag cgcgatgagg ccctccgtga ccacctggag cgccgcctcg tgcaccgctt
     2881 cgagcgtcga accgacggtc gacttttcga tggccgcgag ctgcgcctgc agcacaagct
     2941 cgtagatctt ggcctgggca ggggaaaagc gcccactcgc ggggtacgtc cgcgttacgt
     3001 cggcggcgta gtaaccgtac tcacaaccgg cgtcgacgag cacgagatcc ccctcttcga
     3061 tccggcgatc gttgcggcgg tggtggagga tggttgcgtt gggtccggaa ccgacgattg
     3121 gactgtacgc ggggcgctcg gagccagcgg cgcggaagca gcgccgaagc agtccctcga
     3181 gctcgaattc gtacataccc ggccgggtga ttcgcatgag ctcgcggtga ccctcgttcg
     3241 tgatgtcgac ggcgcgacgc atcagctcga cttcggccgc cgacttacgc aggcgcatct
     3301 cgtggaggag cccatcgggc tcgacgatgc tggttgggta gaccatggcc tgtcgcgtac
     3361 ggcgccgcag cgaagcgatc gctttcagca cgcgcgcgtc ttcgtcctcc gtttggccca
     3421 agcgataaaa gagccggggc tgtccccgca ggagctcggg cagcttcgcg tcgagctcgg
     3481 cgatcggaaa cgcttgatcg gcgccgaagt cgcgcaccgc gccttcgatc ccagcgcgcg
     3541 gtccgtccca cgtttcccgt tccggatcgc gcggacgaac gaagagcgtc gccttcgcct
     3601 ccgcatcgct tcggagcagg agcacgcttt cgggctcgtc gaagccagtc aagtagaaga
     3661 aatccgagtc ctggcggtac tcgtgctcta cgtcgttgtt tcgaatcgca actggtgcag
     3721 cgaagagcaa cagcgcgccg gggtggattg cctcgagaac tcggctctgc ctcgtacgga
     3781 actcatcctg taacgtcacc gactcatttt accatcggtt cggccagccc gtcccgcgcc
     3841 ggaacgaagt ccgaggcgaa cgcaaacgcc ccgcaggggt ttgacgtcgc ccctgctcca
     3901 cggtacgagg cccccgcgtg gcaacggaac gtccgcccga aggcgacgat cgcgagcgcg
     3961 agcagcgtcg gctcgagcga ctgatccccg agctcttgag acgcgtgctg gaggtcggct
     4021 acgagaagct caccgagggg cccgagagcg ttcggaactt ggtctcggag ctcaagctac
     4081 cgaaggaggc gctcggcgtc ctcatctcgc agctcgacga gactaagaac ggtctttacc
     4141 gcgcggtcgc aaaagaggta cgcgactttt tggagcagac gaacttcgcc gaagagctgg
     4201 cgaaggtttt gacgacgctc tcgttcgaag tcaaaacaga ggttcgcttc ataccgaacg
     4261 atcagcgact gcgcgcgacg cccgacatcc gtaccaaggt gagcgtcaag cgcgatcggg
     4321 cttcgcagcc cccacccgaa cccaaagcgc cgtcatcgga cacgacagaa tcgcgcccgc
     4381 caaacgactc cgaggagaag actgagtgaa acctctgact gctggtagtg aaatcgacgc
     4441 ctggtgcacg aagtgccgga tggatcttgg tcaccgcgtg gtggccatgg tggcgggcgc
     4501 gccgaagcgc gtcgtttgcc tcacctgcgg cgccgaacac aactaccgag ccccccgcaa
     4561 cgagaagcag tcatccggca gcagcgtcca cctgcgatcc aaccgcgaga gcgcgcggcc
     4621 gccagcggcg ccgaaagcga gcgggggcgt tcgcgcggcg gcgaaggcaa agaacgaggc
     4681 cgagcggtac gaaggctggg cttccaaaac actgggccaa gccgtcgacg cgttcactcg
     4741 ctactcgatg gagcgaacct tccgcgtcgg ggagctcgtg atgcacccga agttcggtga
     4801 aggttacgtc gaccaggtgc tcgaaggcgg caaggtgaac gtgatgttcc gcgagggccc
     4861 gaaggtcctc gcccatcgcg tatcttaacg gggtgttgaa aaacggcagc ggctcgggcg
     4921 tatgacggcg gcgttcggtt gtgatgccca cccccgtccg tgctcggcct acggcctgcg
     4981 cgcggccgac ccctccca
//
LOCUS       DASTKS010000499         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1195641, whole genome shotgun sequence.
ACCESSION   DASTKS010000499 DASTKS010000000
VERSION     DASTKS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195641"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFQ35_10400"
     CDS             <1..925
                     /locus_tag="VFQ35_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9931090.1"
                     /translation="QGLGGQPQPIVWPQGSPKGGEGMLESTGARAGASDEQFGSGGAG
                     SIEGEGSGGAGGDSFAANGGALGGLRGFGEGGLALSAGGAPASGGASTGGTFGAGGVA
                     ALGGSGAAGSAQGGSAQGGSAQGGAAQGGAMNGTGGGAGVGGAPCGVFINEVQVGSRD
                     VPADEFVELYNSCDGATSLAGHRLVYRASGGNTDVLLMAFASTDRIAARSFLVLGGAA
                     YSAPAFARWGGGSNGVLAAAAGGLALSVDGSGTVDALGYGNASNAFVEGAAALAPTLG
                     TSIGRVPDGADSNDNWHDFAGTTPTPGAPNK"
     gene            1081..>2461
                     /locus_tag="VFQ35_10405"
     CDS             1081..>2461
                     /locus_tag="VFQ35_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9931091.1"
                     /translation="MNVRFPGDETKLSYLWPSLDVTLLFGAFALVGARRGSTPSWLRP
                     LLVGLFCLVRLVRFGDGVRQNYFHRPFNAYLDLPLAPELVRLLWSTSRHSVFWLGLVA
                     CVGAAVAFVFAVAWGLREGEGFLRDARGRTIFLGVVLALVVLTPFGPRAHGDEEFVGA
                     FAGSVGPRFAREVDFLLHVSGYRSEKLAEVRAAAERTRNADASLERLKSASVLVFLVE
                     SYGATVFDPKLASPETDEALARFEQSLRRDGYSLATGLLDSPVYGGSSWLAQATLATA
                     VQTADEFQFSLLKAEEPPTIADAFRRAGYRTVLVQPGTTRAFPERDFLHFEARYAAFD
                     LGYRGPNFGWAPMPDQFVLDVIHRRELTAPARPVLIEYALVSSHAPWSDQPAVVEWDT
                     LGDGAIFQTVPRVRFDTRLVGSAVREAYLASIRYDFEVLSQYLTERLSSSPLVFVIGD
                     HQPIPNATGH"
BASE COUNT          345 a          775 c          869 g          472 t
ORIGIN      
        1 gcaagggctc ggcggacaac cacaaccaat cgtttggccg caggggtcgc ccaagggtgg
       61 cgaaggaatg ctcgaatcca ctggagcgcg agcaggcgct tccgacgagc agtttggtag
      121 cggcggggcg ggtagcatcg agggggaggg tagtggcggc gccggcggag actccttcgc
      181 tgcgaatggc ggcgcgctcg gcgggctccg cgggttcgga gagggagggc tcgcgctctc
      241 tgcaggcggg gcgcccgcga gcggcggcgc ttcgacgggc ggcacgttcg gcgccggggg
      301 cgtcgcggct ctcggcggaa gcggggccgc tgggagcgca caaggcggga gcgcacaagg
      361 cgggagcgca cagggcgggg ctgctcaagg tggcgcgatg aacggaaccg gtggcggcgc
      421 gggcgtaggc ggcgctccgt gcggtgtctt catcaacgag gtgcaggtgg gaagtcgcga
      481 tgtgccagcc gacgagttcg tcgagctcta caactcgtgc gatggcgcga cttcgctagc
      541 tgggcatcgg ctcgtttacc gcgcctctgg cgggaacacg gacgttttgt tgatggcgtt
      601 tgcgtcgacg gatcgaatcg cggcgcgctc atttctggtg ttgggcggtg ccgcgtattc
      661 ggcgccggcg ttcgcgaggt ggggaggcgg ttcgaacggc gttctcgctg cggccgcggg
      721 aggacttgcg ctctccgtcg acggaagcgg cacggtcgac gcgctcggct acggcaatgc
      781 gagcaacgcc ttcgtcgagg gcgccgccgc gctcgctccg accctcggga cttcgattgg
      841 cagggtgccg gatggcgcgg actcgaacga caattggcat gattttgcgg gcacgacgcc
      901 gacaccgggc gccccgaata aatgagccgt tccgctgaaa cattccgagg ctacctcggg
      961 gtcggaccga gaccttctgg cgtgagtccg gcctcgagag tgatttcgac gtaagctccc
     1021 gagtagatgg cggttcggct cggaaaattc gctctggcgt ggcttggcct gagcgcgctc
     1081 gtgaacgttc ggtttccggg ggacgagacc aagctgtcgt atctttggcc ttctctcgac
     1141 gtgaccctgc ttttcggcgc gtttgccctc gtcggcgcgc gccgaggcag cacgccgagc
     1201 tggttgcgtc cgctcctggt cgggctcttt tgtctggttc gcttggtgcg ctttggggat
     1261 ggcgtccgac agaactactt ccatcggccg ttcaacgcgt acctcgactt accgctcgcg
     1321 ccggagctcg tgcggctcct ctggtctacg agccgccact cggtgttttg gctcggtctc
     1381 gtcgcttgtg tcggcgcggc ggtggccttc gtgttcgcgg tcgcttgggg actgcgcgaa
     1441 ggcgagggct tcctgcgcga cgcgcgcggg cgcacgattt ttctcggcgt cgttctcgcg
     1501 ctcgtcgtgc tcacgccgtt cggtccacgc gcccatggcg acgaagagtt cgtgggtgct
     1561 ttcgcgggca gcgttgggcc gcgcttcgcg cgcgaggtcg attttctact ccatgtgtcc
     1621 ggttatcgca gcgagaagct cgcggaggtt cgagccgccg ccgagcgcac gcgcaatgca
     1681 gacgcttcgc tcgaacgact gaaatcggcc tccgttctcg tgtttctcgt cgagtcttac
     1741 ggcgcaacgg tcttcgatcc gaagctcgcg tcgcccgaga cggacgaggc gctcgcgcgg
     1801 ttcgaacagt cgcttcgacg cgacggctac tcgctcgcca ccggtttgct cgactcaccc
     1861 gtgtacggcg gcagctcctg gctcgcgcaa gccacgctcg cgaccgcagt ccaaaccgcc
     1921 gatgagttcc agttttcgct gctgaaggcg gaggaaccgc ccaccatcgc ggacgcattc
     1981 cggcgcgccg ggtatcgcac ggtcctggtc cagccgggca cgactcgcgc attcccagaa
     2041 cgcgacttct tgcatttcga agcgcgctac gcggccttcg acctcggcta ccgagggccc
     2101 aacttcgggt gggccccgat gccggaccaa ttcgtgctcg acgtgattca tcgacgcgag
     2161 ctcacggctc cggcgcggcc ggtgctcatc gaatacgcgc tggtttcgag tcacgcgccg
     2221 tggagcgacc agcccgcagt ggtggaatgg gacactctgg gcgacggtgc catctttcag
     2281 accgttccgc gcgttcgctt cgacacgcgg ctcgtcggca gcgctgtgcg tgaggcgtac
     2341 ctcgcgtcca tccgatacga tttcgaggtg ttgagccagt acctgaccga gcgcttgtct
     2401 tcgtctccac ttgtcttcgt gatcggcgat caccagccaa tcccgaacgc gaccggacac
     2461 a
//
LOCUS       DASTKS010000500         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_80392,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000500 DASTKS010000000
VERSION     DASTKS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80392"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFQ35_10410"
     CDS             complement(<1..582)
                     /locus_tag="VFQ35_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931092.1"
                     /translation="MKTRPSLVGAVLAIAAASACSSPSEPGAGMPGATGGRSSWGGAS
                     SSAGAGGSGSSAGFSGARSEGGGSGAAHSGGASSGGAVSLGGADTNGSGGRTASGGRE
                     LGGTSSASGGRATGGSIGQSGASTGGVGSGGRVASGGGQAGASAGGTLQGSEVPQIPQ
                     KPAATVETESFPLGSVTDIADDSAIYANPSAPEL"
     gene            830..1486
                     /locus_tag="VFQ35_10415"
     CDS             830..1486
                     /locus_tag="VFQ35_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9931093.1"
                     /translation="MNSKKVGVVGTGVVGQTLAEGFLRHGYEVMLASRDPSKLADWKS
                     KAGAKASTGTFAEAAKFGDLVVLAVKGDAAEEAVSLCGDGLNGKLVLDTTNPISTSAG
                     PENGVLHFFTTLDSSLMERLQKKVPQAKFVKAFSSVGAYLMVNPKLPSAPSMFICGND
                     ASAKEATRAILDQFGWETEDMGSVEAARAIEPLCILWCIPGFLKNDWTHAFKVLRPAT
                     "
     gene            complement(1562..2617)
                     /locus_tag="VFQ35_10420"
     CDS             complement(1562..2617)
                     /locus_tag="VFQ35_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9931094.1"
                     /translation="MAELTCGDRRLPLAAGETLLDALGRAGVRVPSSCRAGACQSCLV
                     QVTRGVVPERAQVGLKDSLRSKNFVLACQAIPTSDLEVSLEAVEDLEVAARIQTVARL
                     SEDVLRVELRVASPLNHRAGQFVTLIREDGLARPYSIASRSACTEADVTQCRSLRSDS
                     DSTESHLTQAEGELLELHVRVLPSGRMSHWLASEQARGASVRVRGPSGDCFYVAGKPE
                     QPLVLAGTGTGLAPLLGIIRDAIAAGHQGSIDLWHGARNVAGLYLRNELEALAQVHPN
                     LSYHPCVLEGEPGSYRVGKLDELLLSSLKAFDTPRFFLCGDPPLVQKLKRSLFLRGAS
                     LKEIYADAFVSAPDAAA"
     gene            complement(2628..>2828)
                     /locus_tag="VFQ35_10425"
     CDS             complement(2628..>2828)
                     /locus_tag="VFQ35_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931095.1"
                     /translation="GGPNAYTGRDMRSAHAPLVSRGLNDSHVDIVIEHLGATLKELGA
                     NDSQIAAVAALANSVRNDVLSR"
BASE COUNT          524 a          924 c          918 g          462 t
ORIGIN      
        1 gagctccggt gcggaagggt tcgcgtaaat cgcgctatcg tcggcgatgt cagtcacgct
       61 gccgagcgga aacgactctg tctctaccgt tgccgctggc ttttgcggga tttgtggaac
      121 ttcagagccc tgtaacgtcc cgcccgccga tgcgccagcc tgaccaccgc cgctcgcaac
      181 gcgaccgccg cttccgacac caccggtaga cgcaccgctt tggccaatac ttccgcccgt
      241 tgcacgacct ccgctcgcgc tcgacgtgcc gcccagctcg cgaccaccgc tcgccgtgcg
      301 ccccccactc ccgttcgtgt cagcgccgcc gagcgagact gcgccgccac tcgacgcgcc
      361 tcccgaatgc gccgcacccg acccaccacc ttccgaccgc gcgccagaaa agccagcgct
      421 cgagccactg cctcctgccc cagcgctgga gctcgccccg ccccaagagg agcgtccacc
      481 ggtagcgccg ggcataccag cgccgggctc ggacggcgag ctacaggcgc tcgctgccgc
      541 gatcgccaac actgcgccga cgagcgaggg tcgagttttc acgcgaccaa cgaggccgcg
      601 cgcgcacgta tcgaactttg cagccatcga ctccgccgat tcgagcccgg agcttgccac
      661 atgcagcgcg ggcgatcacg tccacgacac gctcgcggcc catccgatgc cgatgtgggc
      721 ccacggtgtt ccgttgaacg tcacgaacct cgagccgagg cgccgttgct cttacgactg
      781 acgaaggaag ggagtagatt gcttcgccca atcgagagga gaccaaacga tgaacagcaa
      841 gaaagttggc gtagtcggaa ccggcgtagt ggggcagacc ttggccgaag gcttcctccg
      901 tcacggctac gaggtgatgc tcgcctcgcg cgacccctcc aagctagccg actggaagag
      961 caaggccgga gctaaggcat cgaccggaac cttcgcggaa gcggcgaagt ttggcgatct
     1021 cgtggtgctc gctgtcaagg gtgacgcggc tgaagaggca gtgtcgctct gcggggacgg
     1081 gctgaacgga aagctcgtgt tggacaccac caatccgatc tcgacgtccg ccggtcccga
     1141 aaacggcgtt ctccactttt tcacgacgct cgactcgtcg ttgatggaac gacttcagaa
     1201 gaaggtcccg caagcgaagt tcgtgaaggc gttctcgtcc gtgggggcct acttgatggt
     1261 gaacccgaag ctgccgagtg cgccatccat gttcatctgt ggcaacgatg cgagcgcgaa
     1321 agaagctacc cgcgcgattc tcgaccaatt cgggtgggag acggaggaca tgggcagtgt
     1381 cgaagcagcg cgcgccatcg agccgctttg catcttgtgg tgcatccccg gcttcttgaa
     1441 gaacgactgg acgcacgcct tcaaggtgtt gcgccccgca acctgaacgg gcgcgctcga
     1501 accgctcgcg tgagaaggcc acgagccgag agctggcgat ctcggcttgg ccttcgactc
     1561 gctaggcggc tgcgtccgga gcgctcacga aggcgtcggc gtagatttct ttcagcgaag
     1621 cgccgcgcaa aaacagcgag cgtttgagct tttgaacgag cgggggatcg ccgcacaaga
     1681 agaagcgcgg cgtgtcgaac gctttcagtg agctcaagag aagctcgtcg agcttgccga
     1741 ccctgtaaga accaggctcg ccctcgagaa cacacggatg gtagctcagg ttcgggtgaa
     1801 cttgcgcgag cgcttcgagc tcgttgcgaa ggtagaggcc agcgacgttg cgggcaccat
     1861 gccagagatc gatggagccc tggtgaccag ccgcgatggc gtcacgaatg atgcccagta
     1921 gaggcgcgag cccggtgccg gttccagcca agacgagcgg ttgttcgggc ttgccagcga
     1981 cgtagaagca gtcacccgaa gggccgcgta cgcgaacgga ggcgccacgc gcctgctcgc
     2041 ttgcaagcca gtggctcatg cgccccgatg gaagaacccg cacatggagt tcgagcagct
     2101 caccctccgc ctgcgtgaga tggctttcgg tcgagtcgct atcgctgcgg agcgaacggc
     2161 actgggtgac gtcggcttca gtgcaggcgg agcgactggc gatcgaatat gggcgcgcga
     2221 gcccgtcttc tcggatgagc gtgacgaact ggcccgcacg atggttcagg gggctcgcga
     2281 cgcgcaactc gacccgcaag acgtcttcag aaaggcgcgc gacggtttga atgcgcgcgg
     2341 ccacctcgag atcttcgacc gcttcgagcg acacctcgag gtcgctcgtg gggatcgctt
     2401 ggcaggcgag cacgaagttc ttcgagcgca gcgagtcttt gagccccacc tgagcgcgct
     2461 cggggaccac gccgcgcgtg acctgcacga ggcaggattg gcacgcgccg gcgcggcaag
     2521 agctcggcac gcgaacgccg gctcgcccga gcgcgtcgag caacgtttcg cccgccgcga
     2581 gcgggaggcg tcggtctccg caagtcagtt cggccatgtc gtctacgtca acgactcaag
     2641 acgtcgttgc gaaccgaatt cgcgagcgcg gcgacggcgg cgatctgcga gtcgtttgcg
     2701 ccgagctcct tgagagtcgc gcccaaatgc tcgatgacga tgtcgacgtg gctgtcgttc
     2761 agcccgcgcg acaccaacgg ggcgtgcgcc gaacgcatgt ctcgacccgt gtaggcgttt
     2821 gggccgcc
//
LOCUS       DASTKS010000501         5860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1419786, whole genome shotgun sequence.
ACCESSION   DASTKS010000501 DASTKS010000000
VERSION     DASTKS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419786"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFQ35_10430"
     CDS             complement(<1..729)
                     /locus_tag="VFQ35_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9931096.1"
                     /translation="MGLLDRLRRSSVPPARPSTELFGDDFQRKLEMLSIVSRRVFSGR
                     LRAERRSKKKGSGVEFADHRDYVPGDDFRYVDWNAYQRFSRLLVRLYEEEEDLGIYFL
                     VDASSSMAFGDRKKFDQARRLCAALAYVGLANLDRVTIVALCDKVVARMPTTRGKGRI
                     FKVFEFLSSLEPGGNTDLGEALTTFVAQHKRRGLAVLISDLYDPAGFEAGINVLRYNK
                     FEPYVLHVVDPQETKPALHGDVVIY"
     gene            complement(734..1735)
                     /locus_tag="VFQ35_10435"
     CDS             complement(734..1735)
                     /locus_tag="VFQ35_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9931097.1"
                     /translation="MSDSETARREVEEFQKKIGALREEIGRIIVGNREVIDGVLSCML
                     SGSHALLEGVPGLGKTMLVRTLAQVMGLEFSRIQFTPDLMPADIIGTVVLDETDDGKH
                     VFEFRKGPVFANIVLADEVNRATPKTQSALLEAMQEHRVTVGRTTHSLEEPYFVLATQ
                     NPLEMEGTYPLPEAQLDRFFFKLEVPFPSREELHTILERTTSEGEPQVKRVLSREDML
                     SMQALVRRVPVERHVRDYAIRVLQATHPNGEGAPAAVTRFVRTGASPRGVQAMLLAAK
                     VRALFEGRFAAAIDDVKASARPALRHRILLSFEGEAEGVRPDTLISDILSSIPTAKD"
     gene            complement(1767..3551)
                     /locus_tag="VFQ35_10440"
     CDS             complement(1767..3551)
                     /locus_tag="VFQ35_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931098.1"
                     /translation="MQRLATWLPLPLGYAAGALALMKFGAASSVAQRALVGVGVLLAV
                     IAAAITIRALLRRPARWAGSLALDEYHGLSDRITTALSLLERPEAERTALSQAAIEDG
                     LAVVDRLDPRRAVPVPIPRELGVSVLLAALLVGALWFEVRVTRVLPPPPAFEPLVMAP
                     DDLELFGEIARQMSEKADDPESLAAIRRFNALIEDIAARRLERKEAFERMSDLESELA
                     KSADIDREARDLGLEGLARELARSGLTKTAAQALEEKRLADAAQALRELAEKLKKKNA
                     VSRAELERLRGAVERASRQSGERLAAIEQRRRELADEKQSLRKKKSEGGKDGDKVDGK
                     LAENQRKLERLERDHDRAERAARQLSDLDRQLAEAARDLAKDSQTGAEDIRRTADELN
                     KVEKRELSEKEKRQLLERLREMKELLRQQGQAGQERLRQMRRFGQRARGGGTGGEEGE
                     QSGKPGQQSGKGMGRGTRIEQVQVPRIVQVPGGTGQSGEGAKGDKPGSGRQAGSGHDE
                     HLAGEATPPPKGETHDVSAAAVDTGQGSASAEVIHGAAERGFVGKAYKDVYVDYQTVA
                     EQSLEHDEIPPGYRFYVRRYFQLIRPRE"
     gene            4173..5735
                     /locus_tag="VFQ35_10445"
     CDS             4173..5735
                     /locus_tag="VFQ35_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931099.1"
                     /translation="MDQNTKTVVLVADDEPSTLAIVSSHLRAKGFSVLEASDGDQAWE
                     LAHEHLPHLVVLDVMMPGMSGWEVCRKIREAVSLAHTGVIMLTGIGENLNEMTSPLYG
                     ADAHVDKPFNFADLDRKVASTLKRRREGAVGRGDDETEKRGSSKKKGDKKDSPVERPT
                     LRAAADPDTRGASRRLERPMPPVKTKPLLKQPAEQQPRTRNTREKAGRASSKKTTSTQ
                     ERARQAPAQRQTRTSTMKEKPRAKTAAATKPAAKTVAKKAPSKGAAPKKAPAKAAAAP
                     KKASKSAAKAAPAKAKASAVKGAKASAKPEKAPAKKAPAKATKAPAKAPAKAAKAPAK
                     APAKAPAKAPAKAPAKAAAPVKKAPAKAEPKKAEPKKAPAKAAKAAPKAPEPPKAKAA
                     KAAPKAPKAEPEKVEEEKPAKAKPAPAKKPAKAAPPPKPEEDEEEGSDDEGDDLDDEG
                     EESRDEDEDEDEGDEGEEDMGDDLDDDADDDGEEDDEESDDEGADDDGGDEDDDADDD
                     GDEEEEEEEEEG"
BASE COUNT         1113 a         1957 c         1903 g          887 t
ORIGIN      
        1 gtagatcacg acgtcgccgt gcagggcggg cttggtctct tgcgggtcga ccacgtgcag
       61 cacgtacggc tcgaacttgt tgtagcggag cacgttgatc cccgcctcga acccggccgg
      121 atcgtagaga tcgctgatca gcaccgcgag cccgcggcgc ttgtgctggg cgacgaaggt
      181 ggtgagcgcc tcgccgaggt cggtgttgcc gcccggctcg agcgacgaca agaactcgaa
      241 caccttgaaa atacgcccct taccgcgcgt cgtcggcatg cgcgccacca ccttgtcgca
      301 cagggcgacg atggtaacgc ggtcgaggtt cgcgaggccg acgtacgcga gcgcagcgca
      361 cagcctgcgc gcctgatcga acttcttgcg atcgccgaac gccatcgagc tcgaggcgtc
      421 caccaagaag tagatgccga gatcttcctc ttcttcgtag aggcgcacga ggagtcgcga
      481 gaagcgctgg tacgcgttcc agtcgacgta tcgaaagtcg tcgccgggca cgtagtcgcg
      541 gtggtcggcg aactcgacgc cgctgccctt cttcttgctg cgccgctcgg cgcgcagtcg
      601 ccccgagaac acacgccggc tcacgatcga gagcatctcg agcttgcgct gaaagtcgtc
      661 gccgaagagc tcggtgctcg gccgcgccgg cggaaccgac gacctgcgga gacggtcgag
      721 aaggcccatc gcctcagtcc ttcgcggtcg gaatcgagct caaaatgtcg ctgattagcg
      781 tgtcgggacg gacgccctcg gcctcgcctt cgaagctcaa gaggatgcgg tggcggagcg
      841 ccgggcgcgc cgaagccttc acgtcgtcga tcgcggccgc gaagcgcccc tcgaacagcg
      901 cgcgaacctt ggccgcgagc agcatcgctt gcacgccgcg cggcgaagcg ccggtgcgca
      961 cgaagcgcgt gacggcggcg ggcgcgcctt cgccgttcgg gtgcgtcgcc tgcagcacgc
     1021 gaatggcgta atcgcggacg tggcgctcga ccggtacgcg gcgcacgagc gcctgcatcg
     1081 acagcatgtc ttcgcgactc aagacgcgct tcacctgcgg ctcgccctcg ctcgtcgtgc
     1141 gctccaaaat cgtgtgaagc tcttcgcgcg acgggaacgg cacctcgagc ttgaagaaga
     1201 accgatcgag ctgcgcttcc ggcagcgggt aagtgccctc catctcgagc gggttctgcg
     1261 tggcgagcac gaagtacggc tcctcgagcg aatgcgtcgt gcgacccacc gtgacgcggt
     1321 gctcctgcat cgcctcgagc aacgcgctct gcgtcttcgg cgtcgcgcgg ttcacctcgt
     1381 ccgcgaggac gatgttcgca aagaccggtc ccttgcgaaa ctcgaagacg tgcttgccgt
     1441 cgtcggtctc gtccaacacg accgtgccga tgatgtcggc cggcatcaaa tcgggcgtga
     1501 actgaatgcg cgaaaattcg agccccatca cttgcgcgag tgtccgcacc agcatcgtct
     1561 taccgaggcc gggcacgcct tccagcagcg catgggagcc cgagagcatg caggagagca
     1621 cgccatcgat cacctcgcga ttgccgacga tgatgcgccc gatctcttcg cgcagcgccc
     1681 ctatcttctt ttggaactct tcgacctctc gtcgtgccgt ttcgctgtcg ctcattgggt
     1741 gtccctcggt gcgggtcgct ctgtggctac tcccgcgggc gaatcagttg aaaatagcgg
     1801 cgcacgtaaa accgatagcc gggcggaatc tcgtcgtgtt cgagcgactg ttcggccacc
     1861 gtttgataat cgacgtacac gtctttgtac gctttgccga cgaagcctcg ctcggccgcg
     1921 ccgtggatga cctctgccga cgcggagccc tggccggtat cgaccgcagc cgcgctcacg
     1981 tcgtgggtct ctccttttgg tgggggggtg gcctcccctg cgaggtgctc gtcgtgaccc
     2041 gagcccgctt ggcgaccact tcccggctta tcgcccttcg cgccctctcc cgactggccg
     2101 gtgccgcccg gcacctgaac gatgcgcggc acctgcacct gctcgatgcg cgtcccgcgc
     2161 cccatgccct tgccgctctg ttgtccgggt ttgcccgatt gctcgccttc ttcaccaccg
     2221 gtgccgccgc ctcgcgcccg ttggccgaag cgacgcattt gacgaagtcg ctcctgcccg
     2281 gcttggcctt gttgtcgtag gagttccttc atttcgcgca ggcgctcgag cagctgtcgc
     2341 ttctcttttt cgctgagctc gcgcttttcc accttattga gctcgtcggc ggttcgccga
     2401 atgtcttcgg cgccggtctg cgaatccttg gcgagatcgc gagccgcttc ggcgagttgg
     2461 cgatccagat cgctcaactg gcgagccgcc cgttccgcgc ggtcgtggtc gcgctccagc
     2521 cgctcgagct tacgctgatt ttccgccagc ttgccgtcga ctttgtctcc gtccttgccg
     2581 ccctcggact tcttcttgcg aaggctctgc ttctcgtcgg cgagctcgcg gcgccgctgt
     2641 tcgatagcag ccaggcgctc cccgctctgt ctgctggcgc gctcgacggc cccgcgtaac
     2701 cgctcgagct cggcccggct gactgcattc ttcttcttca gcttctcggc gagttcgcgc
     2761 aacgcctggg cggcgtcggc caagcgcttc tcttccagcg cttgggccgc ggttttcgtc
     2821 aatccgctgc gagcgagttc acgggcgagg ccttcgagcc ctaggtcgcg cgcctcgcga
     2881 tcgatgtcgg cgctctttgc gagctccgac tcgaggtcgc tcatgcgctc gaacgcttct
     2941 tttcgttcga gccggcgggc cgcaatgtct tcgatcaaag cgttgaaacg ccggatcgcc
     3001 gcgagcgatt ccgggtcgtc ggccttctcg gacatctgcc gggcgatttc tccgaagagc
     3061 tcgagatcat ccggggccat cacgagtggc tcgaaggcgg gcggcggcgg caagacgcgc
     3121 gtgacgcgaa cctcgaacca cagcgcccct acgagcaggg ccgcgagcag aactgacacg
     3181 ccgagttccc ggggaatcgg caccggcacg gcgcgacgcg ggtccagccg gtcgacgacc
     3241 gcgaggccgt cctcgatggc agcttgcgag agggccgttc gttcggcctc gggccgttcc
     3301 agaagtgaaa gcgccgtcgt gatgcggtcg gaaaggccgt gatattcgtc gagagccaac
     3361 gaaccggccc accgagccgg gcgtcgcaac agggctcgta tcgtgatcgc ggccgcgatg
     3421 acggcgagta gcactcccac tcccacgagc gctcgctggg cgactgaact cgccgcaccg
     3481 aacttcatca gcgcgagcgc ccctgcggcg tacccgagcg gcagcggtag ccaagtcgct
     3541 aatcgctgca aaaaagcccg cattcgcagg cggcgctctg cccgagacgc ggcccgacgc
     3601 gcacggcgga acgcctcagc ctgcgccacc cgaccctcca gccccgcccc catcgaggac
     3661 gcgccgcgcg gtccccagag ggggaaaatc gatcgatgcc ccaccgcagg tttttgggtc
     3721 ccaagccccg ggattcgtca cgtctcgcgg acctccctcg acgaacgcgg cgcgcacgga
     3781 aaacagccgc cgggattgat tcggaaagcg gacggccatg agcatcagaa cggcctcggg
     3841 cctgaaaagt tcccgcatcg cgagtcgacc cgagacttgc cccgagcggc tcgcctcaag
     3901 ttaagcaccg acccgagccg cgggacagga ggcgcccgaa gttggtcgcg ctttgacacg
     3961 aaggcctcgc ccacatcggc gcccctcccc tcggagccat ccccgcgacg tgcaggcggg
     4021 cgaccgcagg cgatcggagc gagccacgtc cattgacccg agcccgacga cgtacaggat
     4081 cgcccccgcg gttcctcgcg aaatcgctgt gtgaagccct tgccgagcgt gcggcgctgc
     4141 gctcgaatgg cgagcggtag gccgaggcca gaatggatca gaatacgaag accgtagtct
     4201 tggtggcaga cgacgagccg agcacgctcg cgatcgtttc gagtcacctt cgcgcgaagg
     4261 gcttcagcgt gctcgaagcg tccgatggtg atcaagcgtg ggagctcgcg cacgagcacc
     4321 tgccgcacct cgtcgtgctc gacgtgatga tgcccggcat gagcggttgg gaagtctgtc
     4381 gaaagattcg cgaagcagtc tcgctcgcgc acaccggcgt gattatgctc acgggcatcg
     4441 gcgaaaactt gaacgagatg acgtctccgc tctacggagc agacgctcac gtggacaaac
     4501 cgttcaactt cgccgatctc gatcgcaagg tcgcatcgac cctcaagcga cgtcgagaag
     4561 gcgccgtagg acgcggcgac gacgaaaccg aaaaacgcgg aagcagcaag aaaaaaggcg
     4621 ataagaagga cagcccagtc gagcgaccaa cgctgcgcgc agccgcggat ccagacactc
     4681 gcggagcatc acgcaggctc gaaagaccga tgcctcccgt gaagaccaaa ccactactca
     4741 agcaacctgc cgaacaacag ccgcgcacga ggaacacgcg ggagaaggcg ggccgagcaa
     4801 gttcgaaaaa gacaacttct actcaagaaa gagcgaggca ggcgcctgcc cagcgacaaa
     4861 cgaggacgag caccatgaaa gagaagccga gagcgaagac cgcggcagcc acgaagcccg
     4921 cggcgaaaac cgtagccaag aaagcgccgt cgaagggcgc cgcgccgaag aaggcacctg
     4981 cgaaggcagc tgctgcgccg aagaaggcga gcaagagcgc tgccaaggcg gcccctgcga
     5041 aggcaaaggc ctcggctgtc aagggagcga aagcaagcgc gaagcccgaa aaggcacccg
     5101 ccaagaaggc gccggccaag gcaaccaagg cccccgcgaa agcgccggcc aaggcagcca
     5161 aagcccccgc gaaggccccc gcaaaggcgc cggccaaagc ccccgcgaaa gcgccggcca
     5221 aagccgcggc tcccgtgaag aaggcccccg cgaaagcgga gccgaagaag gccgaaccga
     5281 agaaggcacc ggcaaaggcc gcgaaagctg ccccgaaggc ccccgagccg ccgaaggcga
     5341 aggcagccaa ggccgctccg aaagccccga aagcggagcc cgaaaaggtc gaagaagaga
     5401 agcccgcgaa ggcaaagccc gcgccggcga agaagcctgc gaaggccgcg ccgcccccga
     5461 agcccgaaga ggatgaggaa gagggctcgg acgacgaagg cgacgacctc gacgacgaag
     5521 gcgaagagag ccgcgacgag gacgaggacg aggacgaagg cgacgagggc gaagaggata
     5581 tgggcgacga cctcgacgac gacgccgacg acgatggtga ggaagacgac gaagagagcg
     5641 acgacgaagg agccgacgac gacggtggcg acgaggatga cgacgccgac gacgacggcg
     5701 acgaggaaga agaagaagaa gaggaagaag gctgagtaga agctaggcgc ttcccgctct
     5761 gattgaaggg ctcgcgtgaa caacgcgggc ccttcgtttt tctggtggcc tccgcctcgt
     5821 cggtgcgcga aagcgtgcag cgccggggga ctagctaggg
//
LOCUS       DASTKS010000502         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1865594, whole genome shotgun sequence.
ACCESSION   DASTKS010000502 DASTKS010000000
VERSION     DASTKS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865594"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2368
                     /locus_tag="VFQ35_10450"
     CDS             <1..>2368
                     /locus_tag="VFQ35_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET9931100.1"
                     /translation="QALANAGLSPSEVDAVDAHGTGTTLGDPIEAQALLATYGQGRSA
                     EEPLALGSLKSNIGHTQAAAAVGSVIKMVMAMQHELLPKTLHAGTPSPHVDWSSGSIA
                     LLTETRPWKRTGRPRRCGVSAFGVSGTNGHVILEEAPAEPNETESEPPSSLATTRLEH
                     AQRAPWQLRLLPCVLSGKTESALRERAAALAASWEKRPEWRLRDVASSLTSRANFKHR
                     AVLFASDEGGALEALRALAEGSTPSSAFSGVAPQEAKLAFLFSGQGSQRPGMGHVLYA
                     AFPEFASAFDEVCAEFENHSINSLASLVFAPEGSELAASLDQTQYTQVALFAFEVALY
                     RLFAAWGVAPDYLIGHSIGELVAAHVSGVFSLADACKLVAARGRLMQGCRPGGAMIAI
                     EATEAEIAESLVPYSEELDIAALNGPRSTVVSGHGAAAVVLAELWRGRGRKVKRLRVS
                     HAFHSPHMNEMLPEFRRVAETIEYFAPTIPIVSNVTGKLSSGAQLMDPEYWVRHVRDA
                     VRFVDGVRTLEAKGVTAFVELGPDAVLTALGQACVSDGSAETLTFLSTQRPGRPQVES
                     LTNTLAELYVHGVGVRFREAFRGTDAAQVDLPTYAFEHRRYWLDCTPRSIEMSSNSGD
                     ADRQTSPEPASGLERYRVVWRPIPDAIHTLNGTWLVFAPETHERDFGLLAWLTSGLAS
                     RGARVRLVALSGEIDRARMREEVRTVLGHAGAPAGVLSLFALDDSAHPFYRGLSRGFA
                     HSVLLAQALEEVESSAPLWCVTQGAVSTDDSDPVRYPAQSMAWGLGRVL"
BASE COUNT          398 a          794 c          766 g          410 t
ORIGIN      
        1 gacaagcgct cgccaacgcc ggcttgtccc cgtccgaagt cgatgccgtc gacgctcatg
       61 gcacgggaac gaccctcggc gatccgatcg aggctcaagc actcctcgcg acctacggtc
      121 aggggcgctc agcggaagag ccgctcgcgc tcggttcgct gaagtcgaac atcggccaca
      181 cgcaagccgc ggcggccgtc ggcagcgtca tcaaaatggt gatggcgatg cagcacgagc
      241 tcttgccgaa gacactgcac gcaggcactc cttctccgca cgtcgattgg tcctctggct
      301 caatcgcgct gctcaccgag acaagaccat ggaaacgaac agggcgacct cggcgttgcg
      361 gagtatctgc tttcggcgtg agcggcacga atggtcacgt catcctcgag gaagcgcccg
      421 ctgagccgaa cgagacggag agcgagccac cttcgagtct cgccacgacg cgactcgagc
      481 atgcgcagcg tgctccgtgg cagctccgcc tcttgccctg cgtactctcc gggaaaaccg
      541 agtccgcgct gagagaacga gccgcagcgc tcgccgcgag ctgggaaaag cgccccgagt
      601 ggcgccttcg cgacgtcgcg tcctcactga cgtcgcgtgc gaacttcaag catcgcgccg
      661 tgctcttcgc cagcgacgag gggggggcgc tcgaggccct gcgtgccctc gctgaaggca
      721 gcacgccgag cagtgcgttc agcggagtcg cgccgcaaga agcgaagctc gcgtttctct
      781 tcagcggcca gggcagtcag cgccccggaa tgggtcacgt cctctacgcg gcgttcccgg
      841 aatttgcgag cgcgttcgac gaagtctgcg ccgagttcga aaaccattcc atcaactcgc
      901 tcgcgtcgct ggtgtttgcg cccgagggct cggagctcgc cgcgtcgttg gatcagaccc
      961 agtacacgca ggtagctctc ttcgcgttcg aggtcgccct gtatcggttg ttcgcggctt
     1021 ggggcgtcgc ccccgactac ctcatcgggc actcgatcgg cgagttggtc gctgcccacg
     1081 tgagcggcgt tttttcgctg gcagacgctt gcaagctcgt cgccgcgcgc ggccggctga
     1141 tgcaaggctg tcgtcccgga ggagccatga tcgcgatcga agcgacggaa gcggagatcg
     1201 ccgagtcact cgtgccgtat tccgaagagc tcgacatcgc agcgttgaac ggcccgcgct
     1261 ctacggtggt ttccggccac ggcgcggcgg cggtcgtgct cgcggagctc tggcgcgggc
     1321 ggggccgcaa ggtgaagcga ttgcgagtga gccacgcgtt tcactctccg cacatgaacg
     1381 agatgttgcc cgagttccga cgagtagcgg agacgatcga gtactttgcg ccaacgattc
     1441 cgatcgtgtc caacgtgacg ggtaaactct ccagcggagc ccagctaatg gaccccgagt
     1501 attgggtccg ccacgtccgg gacgcggttc gcttcgtcga cggagtacgg acgctcgagg
     1561 cgaagggcgt aacggcgttc gtcgaactcg gtcccgacgc cgtcctcacg gcactcggac
     1621 aggcttgcgt cagcgatggc tccgccgaaa ccctgacgtt cctttcgacc caacgacccg
     1681 gacgcccgca agtggagagc ctgacgaaca ctctcgcaga gctttacgtg cacggcgttg
     1741 gcgtgcgctt tcgcgaagcg tttcgcggaa ccgacgcggc tcaggtcgat ctaccgacct
     1801 acgcgttcga gcaccggcgc tactggctcg actgcacccc ccggagcatc gaaatgagct
     1861 cgaattccgg cgacgctgat cggcaaacgt cccccgaacc cgcttctggg ctcgagcgtt
     1921 accgcgtcgt ttggaggccg atcccagacg cgatccacac cttgaacggc acctggctcg
     1981 tattcgctcc cgaaacccac gagcgcgact tcggtctgct cgcctggctg acatcggggc
     2041 tcgcgagtcg cggcgcacgc gtccggctcg tcgcgctatc gggcgagatc gaccgcgccc
     2101 gtatgagaga agaggtgcgg acggtgctcg gccacgctgg cgcgcccgcg ggcgtcctct
     2161 cgttgttcgc cttggacgac agcgcgcatc cgttctatcg aggcctgtcg cgcggcttcg
     2221 cgcacagcgt cctcctcgcg caggcgctcg aagaggtgga gtcatccgca ccactctggt
     2281 gcgtgaccca aggggcggtg tcgaccgacg actcggatcc agtccgctat ccggctcaat
     2341 cgatggcatg gggactcggg cgcgtcct
//
LOCUS       DASTKS010000503         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_972326, whole genome shotgun sequence.
ACCESSION   DASTKS010000503 DASTKS010000000
VERSION     DASTKS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972326"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFQ35_10455"
     CDS             <1..737
                     /locus_tag="VFQ35_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931101.1"
                     /translation="LEGTLREAAGARGLPALLAEAAATEPARLAARLDELDGLIEQLE
                     ERHSDCISAQQRLQAGLELFSDTSAAEAAEEERALASALVSRSERWAKLKLAEVLLSR
                     EVERYREQNQGPVLRRAAELFTRLTQDDYRGLRVGREERVLVAVRANDLEVGVEGLNE
                     AARYHLYLALRLASLERYLEHAEPLPLVLDDVLIHFDEDGARAALDVLGELAQRFQIL
                     LFTHHRHNVALAESATPRERLFIHEL"
     gene            complement(782..1465)
                     /locus_tag="VFQ35_10460"
     CDS             complement(782..1465)
                     /locus_tag="VFQ35_10460"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HET9931102.1"
                     /translation="MSRAHLCPLPLLILCVACNSPPQNPPAASPAQTAPATEPAHTLP
                     ADSTASPSPSASASAQPVPSAETESPFPKSPPELQVPPNSSLVLKARGKGVQIYECAS
                     KPDDARAFAWKLKAPEADLFDDHGEKVVHHFAGPTWQAKDGSSAVGTMMAKADAPDPE
                     AVPWLLLTAVGKGPGLFARVMHVQRLDTSGGKAPATGCDAARAKAKAEVRVPYEATYY
                     FYALTPGSK"
     gene            complement(1587..>2459)
                     /locus_tag="VFQ35_10465"
     CDS             complement(1587..>2459)
                     /locus_tag="VFQ35_10465"
                     /inference="COORDINATES: protein motif:HMM:NF020483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HET9931103.1"
                     /translation="LVGERGQSLSGGERQRIAIARAILVDPRILILDEATSAVDVQTE
                     RDIQRALDNVVSGRTTFAIAHRLSTLEKADLLVVIKDGKLAEIGTKAELLALDGEYAK
                     LHRAQAKAAKNREADAEEEGELPSLPTTTEPLYDVLRLGLEWDSQAHLWAVPAEGGER
                     RRVTPRRCFPLTDPEHFISLIDDQGRERACIDDRSILDEPSRRALEQALEANEFLPRV
                     VRILKVKVAATRSAWQVETDRGRRDFVIEQEDHIRRLEDGRHVITDSHGMRYLVPSPE
                     RLDPVSRKWLARFS"
BASE COUNT          377 a          825 c          839 g          418 t
ORIGIN      
        1 gcctcgaggg cacgctccgc gaggccgcgg gcgctcgagg gctccccgcc cttctcgccg
       61 aggccgccgc caccgagccg gcccggctcg cggctcgcct cgatgagctc gacggcttga
      121 tcgaacagct cgaagagcgc cactcggatt gcatcagcgc tcagcagcgt ttgcaggctg
      181 ggctcgagct cttctccgac accagcgccg ccgaagccgc cgaagaagag cgcgccctgg
      241 cctcggcgct cgtctcgcgt tccgagcgct gggcgaagct caagctcgcc gaggtgctgc
      301 tctcccgtga agtcgagcgc taccgcgaac aaaaccaagg cccggtgttg cgtcgagccg
      361 ccgagctgtt cacgcggctg acccaagacg attatcgcgg cctccgcgtc ggacgcgaag
      421 agcgcgtgct ggtcgcggtg cgcgcgaacg atctcgaggt cggagtcgaa gggctcaacg
      481 aggccgcccg ctaccacctc tacttggccc tgcgtctcgc gagcctcgaa cgctacctgg
      541 agcacgccga gcccttgccc ttggtcctcg acgacgtgct gattcacttc gacgaggacg
      601 gcgcgcgcgc cgcgctcgac gtgctcggcg agttggcgca gcgcttccaa atcctgttgt
      661 tcacccacca tcgtcacaac gtggcgctgg ccgagagcgc cacgccgcgt gaacggctct
      721 tcatccacga gctttgacga gcgaagcgcg agctcgaagc cgccgcacgc ccgaggcccg
      781 tctacttgga acccggtgtg agcgcgtaga agtagtacgt agcctcgtaa ggcacgcgaa
      841 cttccgcttt cgccttcgct ctggccgcgt cgcatcccgt cgcaggggcc ttgccgccgc
      901 tcgtgtccag tcgctgtacg tgcatcacgc gagcgaacag gcccggtccc ttgcccaccg
      961 cggtcagcag cagccaaggc accgcctctg gatcgggagc gtccgccttc gccatcatcg
     1021 tgcccacggc gctgctgccg tctttcgctt gccaggtggg accggcgaag tggtgcacga
     1081 ccttctcgcc gtggtcatcg aacagatctg cttccggggc cttgagcttc caggcgaacg
     1141 cacgcgcgtc gtccggtttg ctcgcgcact cgtaaatctg aacgcctttg ccgcgcgcct
     1201 tgagcaccaa gctcgaattg ggcggcactt gcagctcggg aggagacttc gggaacggtg
     1261 actccgtctc cgcactcggc acgggctgcg ccgatgcgct cgccgaagga ctcgggctcg
     1321 ccgtcgagtc ggccgggagc gtgtgggcag gctccgtcgc gggtgccgtc tgggcggggc
     1381 tcgctgcggg tggattctgc ggcggcgagt tgcaggcaac gcaaaggatc aaaagcggca
     1441 acgggcagag gtgagcacga ctcatggcgg cactttcggg cgggagccgc cgccgtgcaa
     1501 ggcggatcgg gagcttttcg gcaacggctc gcgttcgcgg tcgcacgaat ggcgtccgtc
     1561 ccctcgcggg ggctgtcgac cagctctcaa gaaaagcgcg cgagccactt gcggctgacc
     1621 ggatcgagcc gctccgggct tggaacgagg tagcgcatgc cgtgcgaatc ggtgatgacg
     1681 tgacgcccgt cctcgagcct ccggatgtgg tcctcctgct cgatgacgaa gtcacgccga
     1741 ccgcgatcgg tctcgacttg ccaagccgag cgcgtcgccg cgaccttgac cttgaggata
     1801 cgaacgacgc gcggcagaaa ctcgttggct tcgagggcct gctcgagcgc gcgccgcgaa
     1861 ggttcgtcga ggatgctccg gtcgtcgatg caagctcgct cgcggccctg gtcgtcgatg
     1921 agagagatga agtgctcagg gtcggtgagc gggaaacagc gtcgcggagt gacgcgacga
     1981 cgctctccgc cctcggctgg aacggcccag aggtgggcct ggctgtccca ctcgagcccg
     2041 agccgaagca cgtcgtagag cggctcggtc gtggtgggca aagacgggag ctcaccttct
     2101 tcctcggcgt ccgcctcgcg gtttttcgcg gccttggcct gcgccctgtg aagcttcgcg
     2161 tattcgccgt ccagggcgag gagctcggct ttggtaccga tttcggcgag cttgccgtcc
     2221 ttgatgacga cgagaaggtc cgccttttcg agcgtgctga ggcgatgcgc gatggcaaac
     2281 gtggtgcgcc ccgaaacgac gttgtcgagt gcgcgctgaa tgtcgcgctc cgtctggacg
     2341 tcgaccgcag aggtggcctc gtcgaggatc aggatgcgcg ggtcgaccaa gatcgcgcgc
     2401 gcgatggcga tgcgctggcg ctcgccgccc gagagggact ggccgcgttc acccacgag
//
LOCUS       DASTKS010000504         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_302918, whole genome shotgun sequence.
ACCESSION   DASTKS010000504 DASTKS010000000
VERSION     DASTKS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302918"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFQ35_10470"
     CDS             <1..359
                     /locus_tag="VFQ35_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931104.1"
                     /translation="AKGFRAFSPAPPLEHPTALWADERALPWVLDGSTLLRMTKDSGW
                     ARSEVPFASPLALAGSSSSEVWLAGEGGAALFDGTRFVCIRDLPGPFSGITRAGDDWW
                     FWGAEGAFRLRRTPRD"
     gene            404..1108
                     /locus_tag="VFQ35_10475"
     CDS             404..1108
                     /locus_tag="VFQ35_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931105.1"
                     /translation="MRWLWCSVFSVAAALGCEPKNDVSPAALAATQNPSEPPLVLSGG
                     AKLECTISSRENGRQKLVLDSGQGLEFDVAVSPIVDGIVETSVTNPSASYRFTSHLAA
                     PGQGSLTGVGKVRIEKLETRVRVEMQRYDQPKGPGTELSFRSEDMLNKGVYVEFRGDA
                     RAENGDVYRFRVSLGAAGPGSGGRVQPASNAESAPIAAKMVMIEAPQTTVVSEPVRVS
                     IAPEKRPSETSVRKLP"
     gene            1105..2415
                     /locus_tag="VFQ35_10480"
     CDS             1105..2415
                     /locus_tag="VFQ35_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931106.1"
                     /translation="MKALALAALLVLAACTSPQAAPTAPSTSSEGTAALPSFAPPSAM
                     PARYLRTLEYEPSGYSAERAKRNPTSDLLLGDLLFHSPRTLGPRARSLGISCNSCHPN
                     GAAHVTFELAGLSDRPGNVDLSSAFFRGASDDGIVNFVNIPSLRGCRYTGPYGHDGRT
                     ASLAEFMQSVVHVEFDGAPLSPEHSAALMRYVLDLDFLPNQNLDPRNRLRDGASAAAK
                     RGALLFEAPRTGFGGASCASCHPASTFFRDGQVHRLGSAHSPSPYAIDGGYETPTLLG
                     LAETAPYFHDGRFAHVADVVGWFDVTFSLGLTEAERSDLGAYVEAIGAVDRPRDDRPL
                     AVQLDQVFSYVSLLSLDDQEVCDAARDEVDALLATAPLAMRARARDARKSLGALARSS
                     ERTPAAVSSLREELARLAADWAGALAPCAESPCSVQSKTTVLFP"
     gene            complement(2385..>2712)
                     /locus_tag="VFQ35_10485"
     CDS             complement(2385..>2712)
                     /locus_tag="VFQ35_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET9931107.1"
                     /translation="SADFVTRWQGRLINIHHSFLPAFIGGKPYHQAYQRGVKLIGATA
                     HYATAVLDEGPIIEQDVVRCSHRDSVDDLVRKGRDLEKVVLARAVRWHLDDRILIYGN
                     KTVVFD"
BASE COUNT          438 a          900 c          893 g          481 t
ORIGIN      
        1 aagccaaagg gtttcgcgcg ttctcacccg cgccaccgct cgagcacccg accgcgctgt
       61 gggctgacga gcgagctctg ccctgggtgc tcgacgggag cactctactt cgaatgacca
      121 aggactccgg ctgggcgcgc agcgaggtgc cgtttgcgag tcctctcgcg ctcgccggct
      181 cgagctcgag tgaggtatgg ctcgcaggag aaggcggtgc ggccctattc gatggaacgc
      241 gcttcgtctg cattcgagac ctcccggggc cgttcagcgg tatcacgcgg gccggcgacg
      301 attggtggtt ttggggcgcc gaaggcgcct ttcgcttgcg acgaactccg cgcgactgat
      361 cattgtcgcc ctctgcgtcc gggaaccgac gtatcctctg accatgcggt ggttgtggtg
      421 ctcggtgttt tcggtcgcgg ctgcgctcgg atgcgagcca aagaacgacg tttctcccgc
      481 ggcgttggcg gcgacgcaga acccgtcgga gccacctctt gtcttgagcg ggggagccaa
      541 gctcgagtgc accatcagta gccgcgagaa cggccgccag aagctcgtgt tggactcggg
      601 gcaaggccta gagttcgacg tcgcggtgag cccaatcgtc gacggcatcg tcgagacgtc
      661 cgtcacgaac ccgagcgcct cctaccggtt tacgtctcac ttggcggcgc cgggacaagg
      721 ctcgctcacc ggcgtcggca aggtgcgcat cgaaaagctc gagacgcgcg tgcgcgtcga
      781 gatgcaacgg tacgaccaac cgaagggccc tggtacggag ctctcctttc gctccgagga
      841 catgctcaac aagggcgtgt acgtcgagtt tcgcggcgac gcgcgcgcgg agaacgggga
      901 cgtttaccga ttccgagtga gcctcggcgc cgccggtcct ggctccggcg gtcgtgtgca
      961 accagcgagc aacgccgaaa gcgcgcccat cgcggcaaag atggtcatga tcgaggcgcc
     1021 ccaaaccacc gtcgtgagcg aaccagtgag ggtgagcatc gcgccggaga agcgtccgtc
     1081 cgagacttcg gtccgaaagc tgccgtgaag gcgctggcgc tcgcggcgct actcgtcctc
     1141 gcggcctgca ccagccccca agcggcgccg accgccccga gcacgagttc agaagggacc
     1201 gcagcgctcc caagctttgc gccgccgagc gccatgccgg cgcgctactt gcgaacgctc
     1261 gagtacgagc cctcggggta ctcggccgag cgcgccaagc gaaacccgac ctcggatctc
     1321 ttgctcggcg atcttctgtt tcactcgccg cgcacgctcg gcccacgcgc gcgttcgctc
     1381 gggatttcct gcaacagctg ccatccgaat ggcgccgcgc acgtgacgtt cgagctcgcc
     1441 gggctcagcg atcgccccgg caacgtcgac ctatcgagcg ccttcttccg cggcgcatcg
     1501 gacgacggaa tcgtaaactt cgtgaacatt ccttcacttc gcggctgccg gtacactggc
     1561 ccctatgggc acgacgggcg caccgcttcg ctcgccgagt tcatgcaaag cgtcgttcac
     1621 gtcgagttcg acggcgcgcc gctctctccg gagcattcgg ccgcgttgat gcgctacgtg
     1681 ctcgatctag attttctccc caaccaaaat ttggatccga gaaatcgttt gcgcgatggc
     1741 gcctcggcgg ccgccaagcg cggcgcgctc ctgttcgaag cgcctcgcac cgggttcggc
     1801 ggggccagtt gcgcgagctg tcacccagcg tcgacgttct ttcgcgatgg gcaggtgcac
     1861 cgcctcggca gcgcgcactc gccttctccg tacgctatcg acggtggcta cgaaacaccg
     1921 acattattgg gcctcgctga aacggcgccc tacttccacg acgggcgctt cgcgcacgtg
     1981 gccgatgtgg tcggctggtt cgacgtaacg ttctcgctcg ggctcacgga agccgagcga
     2041 agcgatctcg gcgcctacgt cgaagccatc ggagccgtcg atcgacctcg cgacgaccgc
     2101 ccgctcgcgg tgcaactcga ccaagtcttt tcctacgtga gcctgctttc gctcgacgat
     2161 caagaggtct gcgatgcggc gcgggacgag gtcgatgcgc tactcgccac ggcaccgctg
     2221 gcaatgcggg ctcgggctcg cgacgcgcga aagtctctcg gcgcgctcgc gcgctcgagt
     2281 gaacgaaccc cggctgcggt cagctcactg cgcgaagagc tcgcgcggct cgccgctgat
     2341 tgggcgggcg cgctggcgcc gtgcgcggag tcgccgtgct cggttcagtc gaagacgacg
     2401 gtcttgtttc cgtagatcaa gatgcggtcg tcgaggtgcc agcggacggc gcgcgcgagc
     2461 accacttttt cgaggtcgcg acctttgcgc acgagatcgt cgacggaatc gcggtgagaa
     2521 cagcgcacga cgtcttgttc gatgatgggg ccctcgtcga gcacggccgt ggcgtagtgg
     2581 gcggttgcac cgatgagctt cacgccgcgc tgatatgcct ggtggtacgg cttgcccccg
     2641 atgaacgctg gcaaaaacga gtggtggatg ttgatgagcc gcccctgcca acgcgtgacg
     2701 aagtcggcgc tc
//
LOCUS       DASTKS010000505         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_302958, whole genome shotgun sequence.
ACCESSION   DASTKS010000505 DASTKS010000000
VERSION     DASTKS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302958"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFQ35_10490"
     CDS             complement(<1..579)
                     /locus_tag="VFQ35_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931108.1"
                     /translation="MPSPDALRPAAVPQAATFLEQEREWAYGPFDGDGQRHGAWRFYS
                     EAGALVRIAAYERGIPSGLCRRYEEGDVVEELHYVNGVVDGPYWRRLGADTYRCETAA
                     FEVGTFDRGVRVGTVRLLDAGDQETARFELGRSAVDDDILKVGVLSIFPRTMTAWLEL
                     AERAFEEGRNEEGLASLARAVAKGADPALLERA"
     gene            801..>2690
                     /locus_tag="VFQ35_10495"
     CDS             801..>2690
                     /locus_tag="VFQ35_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9931109.1"
                     /translation="MPLSTSLPASSHDEQVEPNATERALRLAEKLASGAAKAAWPWVQ
                     RLNKAIERPAPTPQWAPGPLLKRRERTFPTLGWPRETDSLCPSCVKEVRGDILAGKRA
                     VSELVEGKPGEIKARIVEEGGVIKMKKTCDRHGEVDDVISIDPAFLARIERLYPGRDY
                     LAPLSELRNHGSSSVKYGRGAVLTVDLTNRCNMMCDPCFMDANQVGYVHELSWDDVQK
                     LLDDSMTIKPRRQMSVQFSGGEPTLSPHFLRAIRYAREVGYFCVQCATNGIRFAQDPE
                     FAKQAKEAGLRLAYLQFDGVTNEANSHRKVGNLFDVKLRAIENLHAAGIDVVLVVTVV
                     NGVNNDQVGPVIDFAIANADKITVVSFQPVSFTGRDEDISDELRHAQRYTLSHLAHDI
                     KDQLGVTEPTRDWFPLSAMGPFSDLTDLMMDSKSDWGALKCGCHPNCGIGTILFVNKR
                     TKQMVPLMDFLNVEQLLADIGDITDAAQGRAITLTELVLALFKNFDARRAPPGFTFYH
                     LVKQFLSQTGNRGKQVGQFESDAHEFEWRVLFVAGMWFQDLWNYDFRRTEMCIIPYGT
                     QAGEISFCAYNTGVGWRNIIEKMHMTASVADWYKDHGRHPVYAKNQALPLPGGRPDIV
                     MPESAL"
BASE COUNT          487 a          935 c          841 g          427 t
ORIGIN      
        1 cgcgcgctcg agcagcgcag ggtccgcgcc cttggcgacg gcgcgcgcga gcgacgccag
       61 gccctcttcg ttgcggcctt cctcgaacgc gcgctcggcg agctcgagcc acgcggtcat
      121 cgtccgagga aagatcgaga gtacgccgac cttgaggatg tcatcgtcga cggcggagcg
      181 acccagctcg aagcgcgccg tctcctgatc gcccgcatcg agcagacgga ccgtgcccac
      241 gcgcacgccg cgatcgaacg tcccaacctc gaacgcagcg gtttcacatc gataagtgtc
      301 cgcgccgagc cgtcgccagt acggcccgtc gacgacgccg ttgacgtagt ggagctcttc
      361 cacgacgtcg ccctcctcgt aacggcggca gaggccgctc ggaatgccgc gttcgtaagc
      421 ggcgatccgt acgagcgcgc ccgcttcgga gtagaagcgc cacgcgccgt gtcgctggcc
      481 gtcgccgtcg aacgggccgt aggcccactc gcgctcttgc tcgaggaagg tggccgcttg
      541 ggggacagcg gcgggccgaa gcgcgtccgg ggaaggcata actactttag aagcaacctg
      601 cataccgtgt aggatggggg tgtttctcgg cacttacggt tgggctgaga gcgccgcgcg
      661 gagttcaggt tgacgcctcc gcccaagcgc ctacgaagtc ctgctcgtcg gcgtcgcgtg
      721 atggcgcgca gggttcgttt agactgcgtt ttcgtccgac tcttccgact cgtccggcga
      781 acgcaagctc caaggtgaga atgcctcttt ccacgtcttt gcctgcttcc tctcatgacg
      841 aacaagtcga gcccaacgcg accgagcgcg ccctgcgact cgcagagaag ctcgccagcg
      901 gcgcggccaa ggccgcgtgg ccgtgggtgc agcgcctcaa caaggccatc gagcgccccg
      961 cgcccacgcc acagtgggct cccggtccgc tgctgaagcg ccgcgaacgc acgttcccta
     1021 cgctgggctg gccgcgcgag accgactcgc tctgcccgtc gtgcgtcaaa gaggtccgcg
     1081 gcgacatcct cgccggcaag cgcgcggtga gcgagctcgt cgaaggcaag cccggcgaga
     1141 tcaaagcgcg catcgtcgaa gagggcggcg tcatcaagat gaagaagacg tgcgaccgcc
     1201 acggcgaggt cgacgacgtc atcagcatcg atccggcgtt cctcgcgcgg atcgagcgcc
     1261 tctatcccgg gcgcgactat ctcgcgccgc tctccgagct ccgaaaccac ggaagctcca
     1321 gcgtcaagta cgggcgggga gcggtgctca ccgtcgacct gaccaaccgc tgcaacatga
     1381 tgtgcgatcc atgcttcatg gacgccaacc aggtgggcta cgtgcacgag ctctcttggg
     1441 acgacgtgca gaagctcctc gacgactcga tgaccatcaa gccgcgccgg cagatgagcg
     1501 tccagttctc gggcggcgag cccacgctgt cgccgcactt cctccgcgcc atccgctacg
     1561 cacgcgaggt cggctacttc tgcgtgcagt gcgcgaccaa cggcattcgc ttcgcgcagg
     1621 atccggagtt cgccaaacag gccaaagaag ccggcctgcg cctcgcttat ctccagttcg
     1681 acggcgtcac caacgaagcc aactcgcacc gcaaggtggg caacctcttc gacgtgaagc
     1741 tgcgcgcgat cgagaacctg cacgcggcgg gcatcgacgt cgtgctggtc gtcaccgtcg
     1801 tcaacggcgt caacaacgac caggtgggcc ccgtcatcga cttcgcgatc gcgaacgccg
     1861 acaagatcac ggtcgtcagc ttccagccgg tcagcttcac cgggcgcgac gaggacatct
     1921 cggacgagct ccgccacgcg cagcgctaca cgctgtcgca cctcgcgcac gacatcaaag
     1981 atcaactcgg tgtcaccgag cccacgcgcg actggttccc gctctcggcg atgggcccgt
     2041 tcagcgatct caccgacctc atgatggact cgaagtccga ttggggcgcg ctgaagtgcg
     2101 gctgccaccc caactgcggc atcggaacga tcctcttcgt caacaagcgc accaaacaga
     2161 tggtcccgct gatggacttc ttgaacgtcg agcagctgct cgccgacatc ggcgacatca
     2221 ccgacgccgc gcagggccgc gcgatcacgc tcaccgagct cgtgctcgcc ctcttcaaga
     2281 acttcgacgc gcggcgcgcg ccgccggggt tcaccttcta tcacctggtc aagcagttcc
     2341 tcagtcagac cggcaaccgt ggcaagcaag tgggccaatt cgagagcgac gcgcacgagt
     2401 tcgagtggcg cgtgctcttc gtcgccggga tgtggttcca ggatctctgg aactacgatt
     2461 tccgccgcac cgagatgtgc atcattccct acggtacgca ggccggcgag atcagcttct
     2521 gcgcctacaa cacgggcgtg ggctggcgga acatcatcga gaagatgcac atgaccgcga
     2581 gcgtcgccga ttggtacaag gaccacggcc ggcacccggt ctacgcgaag aaccaagcgc
     2641 tgccgctgcc cggcggccgg cccgacatcg tcatgcccga gtcggcgctg
//
LOCUS       DASTKS010000506         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1642122, whole genome shotgun sequence.
ACCESSION   DASTKS010000506 DASTKS010000000
VERSION     DASTKS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642122"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFQ35_10500"
     CDS             complement(<1..449)
                     /locus_tag="VFQ35_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9931110.1"
                     /translation="MADALISFKGVRKAFGPKVVYSGLDLEVMRSEVLTIVGGSGVGK
                     SVMLKMLIGLLKPDRGSVTFDGEEVTTMKESQLAMVRRRIAMLFQSGALFDSLSVGEN
                     VAYGLEEHFRETMTPEQRAERVAWALGLVDLPGIEQMRPADLSGGMR"
     gene            complement(487..2220)
                     /gene="recJ"
                     /locus_tag="VFQ35_10505"
     CDS             complement(487..2220)
                     /gene="recJ"
                     /locus_tag="VFQ35_10505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9931111.1"
                     /translation="MDSLVAELARITALENTPDYGAELPPPAGAAASALAARLGKTLT
                     VCDWLAQRGFAEAGHIERFLNPKLSELTLPDAMRDRAAAAERLARAVRAGERVVVFGD
                     YDCDGMTATAILTEILRALGGNVTPLLASRFDGGYGLSAAALQRVLAAKPSLVVTCDC
                     GSSDHASLRELRSAGVDCIVVDHHLVPDEPLPALAFLNPHRPDCGFAYKGLASCGLVL
                     SLGAALRAALNPKLDLRQWLDLVAIGTIADVAPLDGDNRALVRAGLRSLAEPKRPGLF
                     ALMQLAKLEPGTPLTSEDIAFRLAPRLNAPGRLGSPETALELMLARDANSAELLAAQL
                     EQASQERKAQQEQMTTEALEEIEREGYADRRAIVLGREGWNHGIVGIVAGRLSERFRR
                     PVVVFGFTQGHGRGSVRGPSGARLHDALSLCVSTVLRFGGHQAAAGVEVELSRLAEFR
                     AAFEAAVLTTTKPETCASTAIENALWLAPSDDPSRVFAELSELEPCGLKNPSPWMLVE
                     GKLVLAREVTGGHLKLELEVGRGRRISGFGVNLGQRAAGLGERVLVGGRLRRDRYRGG
                     DAVELFVERIF"
BASE COUNT          436 a          933 c          811 g          398 t
ORIGIN      
        1 ttgcgcatgc cgccggacag atctgccggg cgcatctgct cgatgccggg caagtcgacc
       61 aagccgaggg cccaggcgac gcgctcggcg cgctgctctg gcgtcatcgt ctcccgaaaa
      121 tgctcttcta gcccgtaggc gacgttttcg ccgacgctca gcgagtcgaa caacgcaccg
      181 ctctggaaca gcatcgcgat gcgccggcgc accatcgcga gctgagactc cttcatggtg
      241 gtgacctctt cgccgtcgaa ggtcacggag ccgcgatccg gcttgagcag gccgatgagc
      301 atcttgagca tcacgctctt gccgacgccc gagccgccca cgatggtgag cacctcgctg
      361 cgcatgactt cgaggtcgag tcccgagtag accaccttgg gcccgaacgc cttgcgcact
      421 cccttgaagc tgatcaaagc gtcggccatg cggacgcatg gttatagcgc gacacccgcg
      481 actggctcag aaaatgcgct cgacgaacag ctcgaccgcg tcgccgccgc ggtaccggtc
      541 gcgccgcagc cgcccgccga ccagcacacg ctcaccgagc ccggccgcgc gctgcccaag
      601 gtttacgcca aacccggaaa ttcgtcggcc gcgccccacc tcgagttcga gcttcaggtg
      661 gccgccggtc acctcgcgcg cgagtacgag cttgccctcc accaacatcc aggggctggg
      721 gtttttcaag ccgcagggtt cgagttcgga gagctccgcg aacacacgcg agggatcgtc
      781 gctcggcgcg agccacaagg cgttctcgat cgcggtcgat gcgcaagtct ccggcttcgt
      841 cgtcgtgagc acggccgcct cgaacgcagc gcgaaactcg gcgaggcgcg agagctctac
      901 ttcgactccg gccgcggcct gatgaccacc gaagcgcagc acggtgctca cacacaagct
      961 gagcgcgtcg tgcagccgtg ccccgctcgg cccgcgcacg gagccccggc catgcccctg
     1021 cgtgaacccg aacacgacga cggggcggcg aaatcgctcg ctcagccgcc ccgcgacgat
     1081 gccgacgatg ccgtggttcc aaccttcgcg accgagcacg atcgcgcggc ggtcggcgta
     1141 gccttcgcgc tcgatttcct cgagcgcttc ggtcgtcatt tgctcttgtt gggctttacg
     1201 ctcttgcgaa gcttgttcga gctgtgccgc gagcaactcg gcgctgttgg cgtcgcgcgc
     1261 cagcatcagc tcgagcgccg tctcgggact gccgagccga cccggcgcgt tcagccgggg
     1321 cgccaagcga aacgcgatgt cctccgaggt gagcggcgtg ccgggctcga gcttcgcgag
     1381 ctgcatcaac gcgaagagcc ctgggcgctt cggctccgcg agtgagcgca gcccagcgcg
     1441 cacgagcgcg cggttgtcac catcgagcgg cgccacgtcg gcgatggtcc cgatcgcgac
     1501 gaggtccagc cattgccgca aatcgagctt cggattcagc gccgcgcgta gggccgcgcc
     1561 gagcgacagc acgaggccac acgaggccag ccccttgtag gcgaagccgc agtcgggccg
     1621 atgcggattc aaaaaggcga gcgccggcaa cggctcgtcc ggcaccaagt gatggtccac
     1681 cacgatgcag tcgacgcccg cgctcctcag ctcacgcaag ctcgcgtggt ccgacgagcc
     1741 gcagtcgcac gtcaccacca agctcggctt cgccgcgagc acgcgctgta gagcggccgc
     1801 ggacaagccg tagccaccat cgaagcggct cgcgagcagt ggcgtcacgt tgccgcccag
     1861 cgcccgcaga atctccgtga gaattgcggt cgcggtcatg ccgtcgcagt cgtagtctcc
     1921 gaatacgacg acccgctcac ccgcccgtac cgcgcgagcc aaacgctccg ccgccgccgc
     1981 ccggtcgcgc atcgcgtccg ggagagtgag ctccgaaagc ttcgggttca ggaaccgctc
     2041 gatgtggccg gcctccgcga acccgcgctg ggcgagccaa tcgcagacgg tcagcgtctt
     2101 cccgagccgc gccgccaacg ccgacgcggc ggctccagcg ggagggggga gctctgcgcc
     2161 atagtcgggc gtgttctcaa gggcagtgat gcgcgcgagc tcggccacga ggctatccaa
     2221 cactgaccga ctagcgtgtc aaccgttcag ggactgcgct cggtcgtaga ttgaccgaac
     2281 cctcatgtat tgctcgaagt gttttggcca agctccccaa gacgcgcggc tatctttcaa
     2341 tctcttccct ttgggtctgt cccgtcgaat caggcgccgc gaccggccga gcagtccata
     2401 cgggcggcac cgagcatcgc gagctccctc tcgaactcgc ccaccgcgcg acgatcgctc
     2461 cctcacgatc gacgatcgct cctcacgaac gacgatcgct ccctcacgat cgacgaccgc
     2521 cgtcgcgaac gacgatcgcc ttcgcgaacg acgcggccgc cgctgctact ctggtgcg
//
LOCUS       DASTKS010000507         5773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_302970, whole genome shotgun sequence.
ACCESSION   DASTKS010000507 DASTKS010000000
VERSION     DASTKS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302970"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..728)
                     /locus_tag="VFQ35_10510"
     CDS             complement(165..728)
                     /locus_tag="VFQ35_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931112.1"
                     /translation="MGDSMSWLERLDDYESGALAEADAFEEELFALAARDEAADLQFF
                     DAVQNELGFLATKKQFGESHSAEEIARVRAENPRAHYIDLGSGGAPVDVEPWPADTNR
                     VIFQIRLDLRGYEQVEVENARPDGTPIITFRGVRCDPNDGNIYGICFENLARLAFANG
                     PVITRVSGVRDGERKVLGKIETRPQSA"
     gene            complement(713..1288)
                     /locus_tag="VFQ35_10515"
     CDS             complement(713..1288)
                     /locus_tag="VFQ35_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931113.1"
                     /translation="MNAAVAQPEPIENLVRLAEHGDSQAAAALCRRFLPAVRSFARRR
                     LRSKDAVDEFAQDVLLRLVEALRQGSIADHERVGGFVLGICKNLALDRARRRERRDAL
                     WEQHKSVLAEFVEVERDVPTLREMKLEDCLAQMSQRARDILRFSYVDAKSHLEIAATL
                     SISEANARVLRHRTLGQLRDCMQKGLPWATR"
     gene            1385..2374
                     /locus_tag="VFQ35_10520"
     CDS             1385..2374
                     /locus_tag="VFQ35_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9931114.1"
                     /translation="MKPKTKKILLAVLTPMALLLVLAGIVEVRARRRFDAPLPDLHAS
                     QDPRIIDRGRYLAYGLAHCVDCHGQQGEPSPPGERVPLSGGKEFRLPVGVVRARNITP
                     DPETGIGRYSDPELARILRYGVHADGRAVLPFMPFANLADDDVVALLSFLRSQKPVRH
                     AVPQHDVNFLGKAVLAFVIQPQGPTETIRTSFPPEASAEYGRYLALNVGNCVKCHTRM
                     DMRTGKYVAPLFSGGAEHPANGDSKKTYVSPNLTPDARFGWLNGWSEEDFVRRFHAGA
                     VHADSPMPWQAFRSIEDDDARAIFRFLRTLPPAPGGPDPKRHEVLVASGATGS"
     gene            2468..3724
                     /locus_tag="VFQ35_10525"
     CDS             2468..3724
                     /locus_tag="VFQ35_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9931115.1"
                     /translation="MTPEVERTIHAVFRMESARLIGGLARMVRDVGLAEELAQDALVT
                     ALEHWPTEGVPENPGAWLMATAKRRAIDELRRRKRLERKHEELGLELDAARDVAARDI
                     EESLDDDVGDDLLRLIFTSCHPVLSTEARVALTLRLLGGLSTDEIARAFLASEPTIAQ
                     RIVRAKRTLAEQNVPFEVPRGPELEARVGSVLEVIYLIFNEGYSATAGDDWMRPALCE
                     DALRLGRILAELMTTRAEVHGLVALMEIQASRSQARVGPSGEPVLLLEQNRARWDQLL
                     IRRGLAALERAEALGGERGPYTVQAAIAACHATARRAEDTDWRRIAELYAELLSIVPS
                     PIVALNRAVAVSMAYGPALGIELVDALRADGALDDYHLLPSVRGDLLEKLGRRQEAQQ
                     EFLRAAELTRNARERALLLGRASALG"
     gene            3738..4301
                     /locus_tag="VFQ35_10530"
     CDS             3738..4301
                     /locus_tag="VFQ35_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HET9931116.1"
                     /translation="MANPITNVVGFDDAPFSHQHRGDVRIFGAVCARTRLDGVVSGLV
                     RRDGSNSARRMAELVEQSQFKTCVRAVLLQGIAVAGFNVVDVQWLSERLALPVVVVAR
                     RKPRLALIHEAVFRTRGAERKWQLIQRAGEMEPCDSLWIQRVGIDHDAARALLRATTL
                     HGNLPEPLRLAHLIAGGVTAGTSRGRA"
     gene            complement(4303..5256)
                     /locus_tag="VFQ35_10535"
     CDS             complement(4303..5256)
                     /locus_tag="VFQ35_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931117.1"
                     /translation="MLLKGCAGLREREVERILATELAVDASHSESVHEPTWVIVTCEE
                     SKVVVEVHDPLSRKIVQRRFDFGTAKAKAQSRLVALAAAELVLASWAELAVLPEPRVA
                     PEGGAASPEQRRLVRDRVVGPRTRTVMLGTDPSVARESRGPKGERIVYGDYVWERLPG
                     KTISRVIALGSIRKFPTHDGQLFGGGARFGTDLLPLHSWAIDALVESGTLGGVKVDSF
                     SAGAMLYFVGESGRHMLARAGAGLRAGVLRGSGEKSSGVVFPAFWGWPMLALAIDARF
                     ESLMVEISSEAGYATLPSRPGSGESSSGVWISAQVGLGLAL"
BASE COUNT          956 a         1897 c         1958 g          962 t
ORIGIN      
        1 gggtcggcat gacgctcgtc attgctgctt gtgacggcga atcgtcgggc cagacctctc
       61 ttggaagtat cgagctggtt cgccgttcgc gcccgagcag acgctgacgc aaagcaatgc
      121 acgtaacgcg gcgtcgcttc gcagcgaagc tcgggggcag tgcctcaggc tgactgcgga
      181 cgcgtttcga tcttacccaa gaccttgcgt tcgccgtctc gcactcccga cacccgcgtg
      241 atgacggggc cgtttgcgaa ggcgaggcgc gccaagttct cgaagcagat gccgtagatg
      301 ttgccgtcgt tcgggtcgca gcgcacgccg cggaaagtaa tgatgggcgt accgtccggg
      361 cgcgcgttct cgacctcgac ttgctcgtag ccgcgtaagt cgagacggat ctgaaagatc
      421 acgcggttgg tgtcggccgg ccagggttcg acgtcgaccg gagcgccgcc ggagccgaga
      481 tctatgtagt gggcacgcgg attctcggcg cgaacacgcg cgatctcttc ggcggagtgg
      541 gactcgccga actgcttttt ggtcgcgagg aaaccgagct cgttttgcac ggcgtcgaag
      601 aactgaaggt cggccgcctc gtcgcgcgcg gccagagcga agagctcctc ctcgaaggcg
      661 tccgcctccg cgagcgcgcc gctttcgtag tcgtcgagcc gctcgagcca gctcatcgag
      721 tcgcccacgg cagccccttc tgcatgcagt cgcgaagctg gcccagagtg cgatggcgca
      781 ggactcgggc gttggcttcg ctgatggaca gggtcgcggc gatctcgagg tggctcttgg
      841 cgtcgacgta gctgaagcgc aggatgtcgc gcgctcgctg cgacatttgc gcgaggcagt
      901 cttcgagctt catctcgcgc agcgtgggca cgtcgcgctc cacctcgacg aactcggcga
      961 gcacgctctt gtgttgctcc caaagcgcgt cgcggcgctc ccgccggcgc gcgcgatcga
     1021 gcgcgaggtt tttgcaaatg ccgagcacga agcccccgac gcgctcgtga tcggcgatgc
     1081 tgccctgccg caacgcttct actaggcgca aaagtacgtc ttgggcgaac tcgtcgacgg
     1141 catccttcga gcgcaagcgg cggcgggcga agcttcgtac cgcggggagg aacctgcggc
     1201 acagggctgc agcggcttgt gaatccccat gttctgcaag ccgaaccaga ttctcgatgg
     1261 gttcgggctg ggcgacagcg gcgttcattc ggccgcccaa tctagcacag ggaacggcgt
     1321 tggcgatttc tgtaacggat ggtctcggtg acgccacttc tgcgcggcga aggcaaaaga
     1381 tccgatgaaa ccgaagacca agaagatcct cctcgccgtt ctcaccccta tggctttgct
     1441 gctcgtcttg gccggaatcg tggaggtgcg ggcgcgtcgt cgcttcgatg cgccgttgcc
     1501 ggacctccac gcaagccagg atccgcggat catcgaccgg ggccggtacc ttgcgtacgg
     1561 actggcccat tgtgtcgact gtcacgggca gcagggggag ccgtcgccgc ccggagagcg
     1621 cgttcccctc agcggtggca aagagttccg gctgccggtc ggcgtcgtgc gcgcgcggaa
     1681 catcactccg gacccggaga cgggcatcgg ccgctacagc gaccccgagc tcgcgcgcat
     1741 cttgcggtac ggcgtgcacg cagacggacg ggccgtgctg ccgttcatgc cgttcgcgaa
     1801 cttggcggac gatgacgtgg tcgccttgct gtcgttcctg cgctcgcaaa agccggttcg
     1861 gcacgcggtg ccccaacacg acgtcaattt cctgggtaaa gcggttctcg cgttcgtgat
     1921 ccagccgcaa gggccgaccg agaccattcg cacgagcttt ccgcccgagg cgagcgccga
     1981 atacgggcgt tacctggcgc tcaacgtcgg caattgcgtg aaatgtcaca cccgaatgga
     2041 catgcgcacg ggaaaatacg tggcaccgct gttctctggc ggagcggagc accccgccaa
     2101 cggcgactcg aagaagacgt acgtttcgcc gaacctgact ccagatgcgc gcttcgggtg
     2161 gctcaacggc tggagtgaag aggacttcgt gcggcgcttt cacgcaggag ccgtccacgc
     2221 cgactcgccc atgccctggc aagcgttccg cagcatagag gacgacgacg cgcgcgccat
     2281 cttccgtttc ttgcgcacgc tgccacctgc ccccggcggt ccagacccta aacgacacga
     2341 ggttctcgtg gcgagcggcg cgaccgggtc atagcgagat tcgaagtgag gcagcgccgt
     2401 acgagcggga tttccacctg tctcgtgcgg cgcttgcgcc gttcgggccg ggtgctctga
     2461 ttgcggggtg acccccgagg tagagcgcac cattcacgcg gtgtttcgta tggagtcggc
     2521 gaggctcatc ggcggcttgg cgcgcatggt gcgcgacgtc ggactggccg aggagctagc
     2581 gcaggacgcg ctcgtgaccg cgctcgaaca ctggcccacc gaaggcgtac ccgagaatcc
     2641 cggcgcctgg ctcatggcta ccgccaaacg gcgagcgatt gacgagctcc ggcgcagaaa
     2701 gcgcctcgag cgaaagcacg aagaactcgg gctcgagctc gacgcggcgc gcgacgttgc
     2761 tgcgcgggac atcgaagagt cgctcgacga cgacgtcggc gacgatttgt tacggctgat
     2821 attcacgtcc tgtcacccgg tgctctcgac cgaggcgcgc gtcgcgctca cgctgcgcct
     2881 gctcggcggg ttgagcaccg acgaaatcgc tcgcgctttt ctcgcttcgg agccgaccat
     2941 cgcccagcgc atcgtgcgag ccaagcgaac actggcggag cagaacgtgc ccttcgaggt
     3001 cccgcgcggg ccggagctcg aggcgcgcgt cggctcggtg ctcgaggtca tttacctgat
     3061 cttcaacgag ggctattcgg ccacggcggg ggacgactgg atgcgcccgg cgctttgtga
     3121 ggacgcgctc cgcctcgggc gaatcctggc cgagctgatg acgactcgag cggaggtgca
     3181 cggcctcgtc gccttgatgg aaatccaggc atcgcgttcg caagcgcggg tggggccgag
     3241 cggcgaaccc gtgctgttgt tggagcagaa ccgcgcgcgc tgggaccagc tcctgattcg
     3301 acgcgggctc gcggcgctcg agcgggccga ggcgctcggg ggcgagcggg ggccgtacac
     3361 ggtgcaggct gcgatcgcgg cttgccacgc tacggcgcgt cgcgccgagg acaccgactg
     3421 gcgacgcatc gccgagctct acgccgagct cctgagcatc gtcccgtctc cgatagtcgc
     3481 cctgaaccgc gccgtcgccg tcagcatggc ctacggtccc gcgctcggga tcgagctcgt
     3541 ggacgcgctc cgggcggatg gggcgctcga cgactatcac ctgctgccga gtgtgcgagg
     3601 ggatctgctc gagaagctcg ggcgcaggca agaggcccag caggagttct tgcgcgcagc
     3661 cgagctgact cgaaacgcac gcgagcgagc gctgctgctc ggccgcgcct ccgcgctcgg
     3721 ctgaccgaga gcgcacgatg gcgaatccga tcacgaacgt cgtgggcttc gacgatgcgc
     3781 cgttttctca ccagcaccgc ggcgatgtgc gcatcttcgg agcggtttgc gctcgcacac
     3841 ggctcgacgg tgtcgtgtcg gggctcgtgc ggcgcgacgg gtcgaactcc gcccggcgaa
     3901 tggccgagct cgtcgagcag agccaattca agacttgcgt gcgcgcggtg ctcttacaag
     3961 ggatcgctgt cgctggtttc aacgtggtcg acgtccagtg gttgtccgag cgcctagcgc
     4021 tgccggtcgt cgtggtcgcg cgccgcaagc ctcggctcgc gttgattcac gaggctgttt
     4081 ttcgaacgcg gggcgcggag cgcaagtggc agctcatcca acgcgccggt gagatggagc
     4141 cctgcgacag cttgtggatc cagcgcgtcg gcatcgacca cgacgcggcc cgcgcgctgc
     4201 ttcgcgcaac cacattgcac ggtaatctac cggaaccttt gcgcctcgca cacctcatcg
     4261 ccgggggggt cacggccggc acgagccgcg gtcgcgcctg aatcaaaggg cgagaccgag
     4321 tccgacttgc gcgctgatcc agacgccgct cgaggactcg ccgctgccgg ggcgtgaagg
     4381 caacgtcgcg tagccagctt cggagctgat ctcgaccatc aagctctcga agcgcgcgtc
     4441 gatggcgagc gcgagcatgg gccagcccca gaacgcgggg aagacgacgc ccgacgattt
     4501 ctcgcccgag ccgcgcaaga ctcccgctcg cagccccgcg ccagcgcgtg cgagcatgtg
     4561 acgccccgac tcgcccacga agtagagcat cgcccccgcg ctgaagctgt ccaccttgac
     4621 tcccccgagc gtccccgact cgaccaacgc atcgatggcc cagctatgca gcggcaggag
     4681 atccgtgccg aagcgagcgc ccccgccgaa gagctgacca tcgtgggtcg ggaatttgcg
     4741 aatcgagccg agcgcgatga cgcgcgagat ggttttcccc gggaggcgtt cccagacata
     4801 gtcgccgtac acgatgcgtt cgcccttggg gcctcggctt tcgcgcgcga cgctcggatc
     4861 agtcccgagc atcaccgttc tcgttcgagg gcccaccaca cgatcgcgca cgaggcgtcg
     4921 ctgctcgggg ctcgcggcgc ccccctccgg ggcgactcgc ggttcgggta acacggcgag
     4981 ctctgcccag ctggcgagca cgagctcggc ggccgctagc gccacgagcc gactttgcgc
     5041 tttggctttt gccgttccga agtcgaaccg acgttggacg attttgcggc tgaggggatc
     5101 gtgcacctcg acgacgacct tgctctcctc gcaagtgacg atcacccacg tgggctcgtg
     5161 cacgctctcg gagtgagatg cgtcgacagc gagctcggtt gcgagaatgc gctcgacctc
     5221 gcgctcacgg agcccggcgc agcccttgag caacactcgg acgacgagca gagcgttcgt
     5281 gggaggtttg ctttcggcgg ctggcctctc cgtttcggcg gccgaggcgg gggcgaccgg
     5341 cggcgcggcg acgctggttg gcggcgattc gtcggcccga gctcgcgtcg ccgcgaggcc
     5401 acccgcaagg agtatccaga ggcatttact gagcgaacac acccacgccc aaaataacac
     5461 cagttccgcg atgggtgcga gttccgtcac acgcgctcgc ggccaaacgg cctgattgcg
     5521 gaaaaaatcg ccgacgagaa ccccgcgtgt cgatcccggc cagcgtcgct tgtcgtccgc
     5581 tagcgagaga cggtggacgc cgctcgcaga aagcgaagct catcatgcga tacctgtgcg
     5641 tgtacaaacc cggcaacgac ggcagcgagg caaagcctcc gagcgcccgc gagatggagg
     5701 agatgggcaa gctcatcgag gacatgaacc gcgcgggtgt gctccttgcg accggcggct
     5761 gccagcccgg cgc
//
LOCUS       DASTKS010000508         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1196129, whole genome shotgun sequence.
ACCESSION   DASTKS010000508 DASTKS010000000
VERSION     DASTKS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196129"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>2367
                     /gene="tssM"
                     /locus_tag="VFQ35_10540"
     CDS             87..>2367
                     /gene="tssM"
                     /locus_tag="VFQ35_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HET9931118.1"
                     /translation="MSILVAIAVVVFLGAAWTISVAFGLSLWIPTLITSAVVFVIALR
                     LALKEYRGARASREIERSLANQAQQFEKRARPDQQADARALALQFEKALQTLKASKLG
                     RGRGGALYALPWYVIIGPPGAGKSTAIRQSGLKFPLLGSKGSVRGIGGTRNCDWWLTN
                     EAVLLDTAGRYATEEDDRDEWLAFLAMLRRARPKRPLNGLIVALSILDLATAGERDLE
                     QLSGRIRDRVDEVMARLEVRLPVYVVLTKCDLLAGFVESFEDLRKAERAQILGFTLPL
                     RDPRELSDQVEELWTRLVQVIERRSLIRVAEARGAAARQAAFGFPQQLEALKANLASF
                     MSLTFVENVYREAPIVRGVYLTSGTQEGSPVDRLRSSMAEAFGLLPAPIEEAARSDSK
                     SYFLSDLFRTVMFPDQDLAAKSTTAERRLRAARFGVALSLVLTAIMLSTVSVRAYVLN
                     RDIMRGAQGELASAMAARDPSASKPASLAALEPIRARLGTLRTWSAGQAPWRYRGGLY
                     QGDALLPHLRSFYATTLRAVLMEPLVFRLERGLADMVQLKAGLQQAPTSQDHAWLYEQ
                     TRAYLLLTQPKEAGEPPLDTELKRWLTSKLVETWVDRDATQVSPADRQAMSEHIAVYL
                     DLLATDPLLGFSRSKGTVAGARELLSRVPMVKLTVDRLVAEIEPLGLDVTFDHLVGAS
                     GLPISASGRVRGAFTRRAWQEQLEPLFKELPTELMGDGWVLESLSHDGRTREARTCAL
                     RSEYFSRYIDEWRAFVGTIR"
BASE COUNT          388 a          702 c          831 g          446 t
ORIGIN      
        1 gtggtctccc tgcgtgagca gcttggccgc ttcgtggtgt ggatgggaca ggcggagctc
       61 tcgaccccgg ttcggacggc gggacgatga gcatactcgt cgcgatcgcg gtcgtcgtgt
      121 ttctgggcgc tgcctggacc atcagcgtcg cctttgggct ttcgctatgg attccgacct
      181 tgatcacgag cgctgtcgtg ttcgtgatcg cgttgcgcct ggcgctgaag gaataccgag
      241 gggcgcgcgc cagccgtgag atcgagcgct cgcttgcgaa tcaggctcag cagttcgaga
      301 agcgcgcgcg gccagatcaa caggcggatg cacgggcgct ggcgctgcaa ttcgagaagg
      361 ccctgcagac gctcaaagcg tcgaagcttg gtcgcggccg cggcggcgcg ctctacgcgt
      421 tgccttggta tgtcatcatc gggccaccgg gcgcggggaa gagcacggcg attcgacaat
      481 cggggctgaa gttcccgctg ctcggcagca agggcagcgt gcgcggcatc ggcgggaccc
      541 gcaactgcga ctggtggttg accaacgagg ctgtgctgct cgacactgcg gggcgctacg
      601 cgaccgaaga ggacgaccgt gatgagtggc ttgcattttt ggcgatgctc cgccgcgcac
      661 gacccaagcg tccgctcaat ggtctgatcg tcgcgcttag tatcctggat ctcgcgaccg
      721 cgggtgagcg ggaccttgag cagctctcag ggcgcatccg ggaccgcgtc gatgaggtca
      781 tggcgcggct cgaggtgcgt ctacccgtct atgtcgtgct gacgaagtgc gatctcttgg
      841 ctggttttgt cgagtcgttc gaggatctgc gcaaggcaga gcgagctcag atcttaggct
      901 ttacgttgcc gctgcgcgat ccacgcgagc tgagtgacca agttgaagag ctgtggacgc
      961 ggctcgtgca ggtgatcgag cgccgcagcc taatccgggt cgccgaggcg agaggcgccg
     1021 ccgcgcgtca ggcggctttt ggatttccgc agcagctcga ggctctcaaa gccaacttgg
     1081 cgagcttcat gagtctgacc tttgtggaga acgtgtaccg ggaagcgccg atcgtgcgtg
     1141 gagtgtacct gacgagcggg acgcaggagg ggagccccgt cgatcgcctg aggagctcta
     1201 tggcggaggc gttcgggctc ctgccggcgc cgattgaaga ggcggcccgg tctgatagca
     1261 agagctactt cttgagcgac ctgttccgga ccgtcatgtt tccggaccaa gacctcgccg
     1321 ccaagagcac cacggcggag cggcggcttc gcgccgcgcg gttcggggtc gcgttgagcc
     1381 tggtattgac ggcgatcatg ttgtcgaccg tgagcgtgcg agcgtacgtg ctcaatcgcg
     1441 acatcatgcg cggcgctcag ggagagctcg cgtccgcgat ggcagcgcgc gacccgagtg
     1501 ccagcaagcc cgcgtcgctg gcggccctcg agccgattcg cgcacggctc ggaaccttgc
     1561 gcacctggtc tgcaggccaa gcgccttggc gttatcgagg ggggctgtac cagggagatg
     1621 ctttgctgcc ccatttgagg agcttctacg cgacgacctt gcgtgcggtg ctcatggagc
     1681 ctttggtgtt ccggctcgag cgtggcctcg cggacatggt gcagctcaaa gctggcttac
     1741 agcaggcacc caccagtcaa gaccacgcat ggctctacga gcagacgcgc gcgtacctcc
     1801 tcctcactca gcccaaagaa gccggcgaac ctccgctcga taccgagctc aagcgctggc
     1861 tgacaagcaa gttggtggag acttgggtgg acagggatgc gacgcaggtc tcacccgccg
     1921 atcggcaggc tatgtcggag catatcgcag tatatcttga cctgctcgcg accgacccgc
     1981 tgctcgggtt ttcgcggtcc aaagggacgg tcgctggcgc gcgcgagctg ctctcgcgtg
     2041 ttcccatggt caagctcacg gtcgaccgtt tggtggctga gatcgaaccg ctcgggctcg
     2101 atgtgacgtt cgatcatctg gtgggcgcga gcggactgcc aatcagcgca agcgggcgtg
     2161 tacgcggcgc gtttacgcgg cgcgcgtggc aggagcagct cgagccgctg ttcaaggagc
     2221 tgcctacgga gctgatgggt gacggatggg ttctcgaaag cctgagccac gatgggcgaa
     2281 cgcgcgaggc gcgcacttgt gcgctgcggt ccgagtactt cagccggtac atcgacgagt
     2341 ggcgcgcctt tgtcggcacg atccgag
//
LOCUS       DASTKS010000509         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_303032, whole genome shotgun sequence.
ACCESSION   DASTKS010000509 DASTKS010000000
VERSION     DASTKS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303032"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFQ35_10545"
     CDS             complement(<1..269)
                     /locus_tag="VFQ35_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9931119.1"
                     /translation="MTTAHLPWFRHASGITTIDAGYVRPGFASVHVLERSGRVAIIDT
                     GTSFSVPLVMKALEELGLQANAVDLLFLTHVHLDHAGGAGQLLERL"
     gene            complement(516..1268)
                     /locus_tag="VFQ35_10550"
     CDS             complement(516..1268)
                     /locus_tag="VFQ35_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931120.1"
                     /translation="MTQRIALITGGSRGLGRSMALQLAARNIDVILTYRSNAKEANQV
                     VAGIAQLGRKAVALPLDVARSATFPAFADTVRSVLADRWNRATFDCLVNNAGTGAFAP
                     FLETTEEQFDQLLDSHLRGTFFLSQKLIPLIADGGRILNVSSGLTRYTYPGQAAYAAM
                     KAGVEVLTRYMALELGPRISVNVIAPGGVETDFAGGAMRDPNLQKVVAAETALARVAK
                     PDDIGGVVAALLAPETAWLNGQRIEVTGGFRL"
     gene            1359..1715
                     /locus_tag="VFQ35_10555"
     CDS             1359..1715
                     /locus_tag="VFQ35_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9931121.1"
                     /translation="MARVIKALPHVPAERALRIIAGRWKVNILYYLLTEPRRLSELTR
                     LLPGASQKVLVQQLRELESHRIVARRVFAEVPPRVEYSLTKLGRSLAPIVGALCRWGT
                     RHSAALDAIDAPEIAS"
     gene            1876..2013
                     /locus_tag="VFQ35_10560"
     CDS             1876..2013
                     /locus_tag="VFQ35_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931122.1"
                     /translation="MQATPGGRHHPQLALQHWVPAEQVTLPQLVGPSGSQVAVPPFAT
                     Q"
     gene            complement(2391..2657)
                     /locus_tag="VFQ35_10565"
                     /pseudo
     CDS             complement(2391..2657)
                     /locus_tag="VFQ35_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          514 a          938 c          937 g          492 t
ORIGIN      
        1 agcctctcga gcagctggcc agcgccgccg gcgtggtcca ggtgcacgtg cgtcaagaac
       61 agcaaatcga ccgcgttcgc ttggaggcca agctcctcca gcgccttcat gacgagcggc
      121 accgaaaagc tcgtccccgt gtcgatgatg gcgacccgcc ccgagcgctc caggacgtga
      181 accgaagcaa acccggggcg gacatacccg gcgtcgatcg tcgtgatgcc ggaagcgtgg
      241 cgaaaccagg gcaagtgcgc cgtcgtcacg tggctaaggg cccgcgaatg aacaagtcag
      301 tcgcgagcgg gccgagaccg cagcgaggcg ggcgcgtgag gagagagcac cgcaggcctg
      361 ctgaagcaga tcgaggagcg caacgacgag cccgcacgcc gcagcaagag gatcggcccg
      421 cgcagtatga cttatccatt cgagggccca aagctaccac gccgagcgag gcggcagaaa
      481 cggtccttcc tgcgccgaac ggaggacgtc atcgctcaca acctgaaccc acccgtgacc
      541 tcgatgcgct gcccattcag ccacgccgtc tccggggcca ggagcgctgc gaccacgccg
      601 ccgatgtcgt ccggctttgc gacgcgcgcg agggcggttt cggcagcgac gaccttctgc
      661 agattcgggt cgcgcatcgc tccacccgcg aagtcggttt cgacgccgcc gggagcgatg
      721 acgttgaccg atatacgagg gccgagctcc agcgccatgt aacgcgtgag gacttcgacg
      781 ccagctttca tcgccgcgta ggcggcttgc cctggatacg tgtagcgggt gagccccgac
      841 gagacgttga ggatgcgtcc cccatcggcg atgagtggga tgagcttttg agaaaggaag
      901 aacgttccgc gcaaatggct gtcgaggagc tggtcgaact gctcttcggt cgtctccaag
      961 aacggggcga acgcgcccgt gccggcgttg ttgacgaggc aatcgaaggt cgctcggttc
     1021 cagcggtccg cgagaacgct tctgaccgtg tccgcgaacg cgggaaaggt ggcacttcgc
     1081 gcgacgtcga gtggtaaggc gactgctttt cggccgagct gagcgatgcc ggctacgact
     1141 tggttcgctt ctttcgcgtt gctgcgatag gtgaggatga cgtcgatatt ccgagccgcg
     1201 agctgcaagg ccatgctgcg acctaaccct cggctcccgc cagtgatgag tgcgatgcgc
     1261 tgagtcatgc tctcaatatg gtgaagggtc gcggaagcgc gagtgcacca attgctgcca
     1321 tacgaaccag aaggtaagat accccttaag aggtaagcat ggcccgcgta atcaaggctc
     1381 tcccgcatgt gcccgccgag cgcgcgctca gaatcatcgc gggtcgttgg aaagtgaaca
     1441 tcctgtacta tttgctgact gagccccgcc gcctgtccga actgacgcgg cttcttccgg
     1501 gtgcaagtca aaaggtgctc gtgcagcagc ttcgcgagct cgagtcgcat cgcatcgtcg
     1561 cgcgtcgagt gttcgccgag gtgccgccgc gcgtcgagta ctcgctgacc aagctcgggc
     1621 ggagcctcgc accaatcgtt ggcgcgctct gccggtgggg tacgcgtcac tcggctgcgc
     1681 tcgacgccat cgacgctccg gaaattgcga gctgaagggc ggccaacgtc agcgccgctg
     1741 acctcgactt cgagggctcg agcttggggg cgacgcgctg cttcgagccg cgagctgcgg
     1801 ggggaacgaa gcggggccgt acgccgtagg gacgacgccg gccccgtgga gctctcctac
     1861 tcgcaggcta tttggatgca agcgacgccc ggcgggcggc accatccaca gcttgcgttg
     1921 cagcactggg tgccggccga gcaagtcacc ttgccgcagc tcgtgggccc atcgggttcg
     1981 caggtcgcgg taccgccgtt tgcgacgcag tgagtgccag ctttacagcg taccgtggag
     2041 caggcgtcgg tgatgggctc gcagcttgcc gtgttgccgc taactacgca ctgtgtgccc
     2101 gtcgggcaga ggatcgccgc gcacgggttc gtgcccgcgt ctggaacgca cgcgcaaacc
     2161 ttgggcgacg tgtcgaagtg cgaaccgatg acacaaagcg cgttcgcaat gcattcgcat
     2221 actccgccgc agtcggcgcc gccactcgca gggtcacagt catcgctcgg gtcgtctacg
     2281 caatggcccg cgcccgggca ttcgatcccc gcaatccccc cacaaaatac gctggggcac
     2341 gcgcccttgc tcgcgacgga cactccggcg gccgcggcct cgcaggcgtt gccgtaggtc
     2401 cgttcgtcgc agccacatac gggcgaatag atgcgtgtgc agatttgggg gcgtgctcgg
     2461 catacgccgc ggccgccgca agctccaacg tccgtcgcac agtagtcgct tttgcgacag
     2521 tctcggtccg acagacagcc gcgtggccga cgcagctcgt caccctgatt cccgacgctc
     2581 tcgtcgcctt cgttcgccgc gctgcatcct gctgccaaga gtaccggcgc ccacagtgca
     2641 aataggaaac gcttcatcga accctccttg tcgaagacac caccgcgacg gagcgcgatg
     2701 ggcggggcga agcctagcgc agcgcaggtt acttgccgtc aacaatctct cggcgatcgt
     2761 gacgctctcg cgcactccac tattgcgcgc tttcgtgttg gagctcctcc accccatcgg
     2821 atttcgtacg gcgatttgcg tcaccctcgc ggtgagagct atcgcgcgcg ccctgcactg
     2881 c
//
LOCUS       DASTKS010000510         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_81178,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000510 DASTKS010000000
VERSION     DASTKS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81178"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFQ35_10570"
     CDS             complement(<1..271)
                     /locus_tag="VFQ35_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HET9931123.1"
                     /translation="MAQAFDLRADSRPFKTDSAASGATTMRVQKRDGSFEPVDLNKIV
                     RAVTRSATGLTRVDPMRVSLKTIGGLYDGATTQALDELSIQTAAGL"
     regulatory      complement(406..603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(739..2328)
                     /locus_tag="VFQ35_10575"
     CDS             complement(739..2328)
                     /locus_tag="VFQ35_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931124.1"
                     /translation="MACSSSGGSSSDGGGNSANGARGGNTNGGASANGGANAGATATN
                     GGVTANGGAATSGGVTANGGTSSSAKGGATASGGANASGGASSGGRANTSGGAAGSDG
                     AGGGSTPTSLDLVAAPDGKDSAPGTLEQPTTLTAALTRVTAGHTIFLRGGTYASAVQI
                     TITRDNSGANGSLKQLVAYGSEHPVLDFSSQPYGSSSNPRGVQINGSYWHFNGLEVKG
                     SADNGIYVAGNYNVIEGCVTHGNRDTGLQLGRASSDLANISDWPSNNLILNCESYDNY
                     DVPPGSGENADGFAAKLTVGPGNVFRGCVSHHNIDDGWDLYTKTDTGPIGPVTIDQCI
                     AYSNGSLTDGTSNDNGDRNGFKLGGDDIAVAHVVTRSVAFKNGKNGFTWNSNPGAIRL
                     SNNLAFDNVEGNFKFGDNSTMTAAVFTNNVSFWTTSGAQSDKAVGTDAGSSNAWWDKS
                     KSQPSVNAKGLVVTAQDFASSLASVKVTRNADGSPNLSVFSLAAGSDLLNAGKLPDGA
                     LPFDAAAYYVGVPDLGAVEAK"
BASE COUNT          440 a          941 c          818 g          531 t
ORIGIN      
        1 tgagcccggc tgcggtttga atcgagagct cgtcgagcgc ttgcgtggtt gcaccgtcgt
       61 acaagccgcc gatcgtcttc aacgaaacgc gcatcgggtc gacgcgagtg agccccgtcg
      121 ccgagcgcgt gaccgcgcgc acgatcttgt tcaagtcgac gggctcgaag ctcccgtcac
      181 gtttttgcac gcgcatggtc gttgccccag aagctgcgct gtcggtcttg aatggacgag
      241 aatcagcgcg cagatcgaat gcctgagcca tcggatctcc ctgccgtttc cgtaaacggc
      301 gagtgaacga atgccgccac gttcacgcgc gcacacgagt gcgtcgcgct gaccacggtg
      361 cgcccacgcg cgagttccaa accgaggtcg aaccgtcgcc cgagcagtcg agcgacgact
      421 tccggcaggt cttcgggctc gtgagcgaaa gcctactatt cgccgcttcc cggcctctta
      481 agagaccagt gcgttcgtgc gaagttcgtt ctcacatacc gctgcgtggg cagccccgga
      541 ttcacaccgg gttcccttat gcgcctgagc acgcgctcag gcaccggaag tgcgcccaac
      601 atattggggt gcctcccgag ctgtcaaccc atcagtaggg gtcccagctg atttctcgtt
      661 cagtcactga accgatggcc ggcccagcgg cgtcgcacct cacagcgaga cgctgcacag
      721 cgagacgccg cgttcgcgtc acttggcttc gaccgcgccc agatcgggaa cgccgacgta
      781 gtacgcggcc gcgtcgaagg gcaacgcgcc gtccggcaac ttgcccgcgt tcaggagatc
      841 gctacccgct gcgagtgaaa acactgaaag attcggactg ccgtcggcgt tacgcgtgac
      901 cttcacgctg gcgagcgagc tcgcaaaatc ctgcgccgta acgactagcc ctttcgcgtt
      961 cacgctgggc tgcgacttgg acttgtccca ccaagcattg ctgctgcccg catcggttcc
     1021 aacggcttta tcactctggg cgcccgacgt cgtccaaaac gacacgttgt tcgtgaacac
     1081 ggccgccgtc atcgtgctgt tgtcgccgaa tttgaagttc ccttccacgt tgtcgaacgc
     1141 caggttgttg ctcaagcgaa tcgcgccggg attgctgttc cacgtgaagc cgttcttgcc
     1201 gttcttgaac gccaccgagc gcgtcacgac gtgggcgacc gcgatgtcgt caccgccgag
     1261 cttgaagccg ttgcgatcgc cgttgtcgtt gctcgtccca tcggtgagcg agccgttcga
     1321 atacgcgatg cactgatcga tcgtcaccgg cccgattggg cccgtatccg tcttcgtgta
     1381 aaggtcccaa ccgtcgtcga tgttgtggtg cgacacgcag ccgcgaaaca cgttgccggg
     1441 gccaaccgtg agcttggccg caaatccgtc cgcgttctct ccgcttccag gcggcacgtc
     1501 gtagttgtcg tacgactcgc aattcaaaat caggttgttg gaaggccaat cggaaatgtt
     1561 cgccaagtcg gagctcgcgc ggccgagctg caaccccgtg tcgcggttgc cgtgggtcac
     1621 gcagccctcg atgacgttgt agttgcccgc cacgtagatg ccgttgtcgg ccgagccctt
     1681 cacctcgagc ccattgaagt gccaatagct cccattgatt tgcactccgc gcgggttcga
     1741 gctcgagccg tagggctggc tcgaaaagtc gagcaccgga tgctcgcttc cgtaggcgac
     1801 gagctgtttc aagcttccat tcgcgccgct gttgtcgcgt gtgatcgtga tttgcaccgc
     1861 cgacgcatac gttccgccac gcagaaaaat cgtatgcccc gccgtcacgc gagtcagcgc
     1921 tgcggtcaaa gtcgttggtt gctcgagcgt gccgggcgcg ctgtctttcc catctggcgc
     1981 cgcgacgaga tcgagcgacg ttggcgtcga gccgccgcct gcgccgtccg aacccgcagc
     2041 gccgccactc gtattcgcgc ggcctccaga gctcgctccg ccgctcgcgt tcgcgccgcc
     2101 gctcgcagtc gccccgccct ttgcgctcga actcgtgccg ccgttcgcag tcacgccgcc
     2161 gctcgtcgca gcgccgccgt tcgcagtcac gccgccgttc gtcgcggtcg cgcccgcatt
     2221 ggctccgccg ttcgcgcttg ctccaccgtt ggtgttcccg ccacgagcgc cgttcgcgct
     2281 gttgccgccg ccgtccgaac tcgaccctcc actcgacgag cacgccacga ataacggaag
     2341 gagcagaaac cttgcagtgc ttcgggacaa catcactctc gcgaaccttt cggtgctcgc
     2401 ccgtgtcgaa cggagcggga cgaattctcg actcttcaca agtggcgcga gccgtcgctg
     2461 acggttcacc cgccgagctc ccaactcgaa gctcgcggag tacgacgccc ggcgacgcac
     2521 ctgatgggcc gcgaattcga ggagatttga tgcccacccc gcgccatgct cggggcttcg
     2581 cccctgcgcg tggcgccccc tcccaagcgt cgcgtcccgc gccgcgggag gggtcacggc
     2641 gcacgctgcg caccggcgca gccgacgttt cacgcgtcgg tgagcttcac tctgcgcgcg
     2701 ttgctcgctg tggcagcgga cgtttcatgc
//
LOCUS       DASTKS010000511         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1196471, whole genome shotgun sequence.
ACCESSION   DASTKS010000511 DASTKS010000000
VERSION     DASTKS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196471"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFQ35_10580"
     CDS             complement(<1..324)
                     /locus_tag="VFQ35_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931125.1"
                     /translation="MVKVAFRATAKAYEGEASSSSLLFVAWPLVLLIGAAMRDLRWLF
                     GFLGALLVVGCGDDGGSGGSGGAGGSGGGGGAGACVGGGQGTLNVNIRIDTGVPASVT
                     FKSDTG"
     gene            complement(349..1083)
                     /gene="deoC"
                     /locus_tag="VFQ35_10585"
     CDS             complement(349..1083)
                     /gene="deoC"
                     /locus_tag="VFQ35_10585"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9931126.1"
                     /translation="MTAADHAEIAKLIDHALLLPNLDRSALESGLALARSYEVASVCI
                     LPYYVRRAREALAGSSVLTSTTIGFPHGATSTRAKLAEAEAALDDGAEELDALVNVSQ
                     VLSGEWQSVAREVESLVTLCHQRGKKLKSIFENAYLDDGQKRTLCRICGELGVDWVKT
                     STGFGPSGATLPDVELMRAESPPHVQVKAAGGIRDLDTLLRFRPLVTRIGTSHTQTIL
                     DDCRRRMGLPAMRLDSSVGGGTSSAY"
     gene            1146..>2526
                     /locus_tag="VFQ35_10590"
     CDS             1146..>2526
                     /locus_tag="VFQ35_10590"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9931127.1"
                     /translation="MQPFRFWRRSLETNAFGAALVLALGCGATDSTKSPADGQNELAE
                     RDPAELYDWDHVPTFELSLPPGEFEHLLATAADEVYTRASLTFDQKPIGDVGLRFKGS
                     VGTLESCFDAQGNLTCPKLSMKLGFDEYVPSTRFFRQKRLNLHSMVRDDTKLHERLAY
                     DLYRSMGIVAPRSAWAVVKVNGESLGLFSMVEQIDGRFTANRWPGAGDGNLYKEAWPL
                     STDAQYYEDHLETGASQPDAAPHSAYVDFTSALSAGGDDKGRGALGRYMDLEYLSRYM
                     AVDDAILNVDGVTATYTGDDPRYYANHNFYVYQEEQRGFFWLVPWDMDATFNLRGDFE
                     LVPRWNGSGADCTSRYTVWGAARVVSPACDHLFQALASDPSSYQAAVDELLAGDFSEA
                     KLNETIDRHAAFIASAVEHDPKGAGLSAWQGAVSYLKRQLPLVRAHLGRAKLGLSSKP
                     LMLAAESVTD"
BASE COUNT          470 a          863 c          772 g          421 t
ORIGIN      
        1 gccggtgtcg ctcttgaagg tgacactcgc gggcaccccg gtatcgatgc gaatgttcac
       61 gttgagcgtg ccctggccgc cgccgacgca agcgccagcg ccgccgccac ccccggagcc
      121 gccagcacca ccgctaccgc ccgagccgcc gtcatcgccg cagcccacta ccaaaagcgc
      181 tcccaagaaa ccgaagagcc aacgcagatc tctcatggca gcgccgatga gtaacacgag
      241 cggccacgcc acgaacagca acgaactcga ggaggcttcg ccttcgtacg cttttgcggt
      301 cgccctgaac gcgactttca ccacatgagc ggcgacttca atacatgctc aatacgcgct
      361 gctcgtgccg ccgcccacgc tcgaatcgag ccgcatcgcg ggcaaaccca tgcggcgtcg
      421 acagtcatcc aggatggttt gcgtgtggct cgtcccgatg cgagtcacga ggggccggaa
      481 gcgcaacagg gtatcgagat cgcgaatgcc tcccgccgct ttcacttgga catgcggagg
      541 gctttcggcc ctcatcaact cgacgtccgg cagcgtcgct ccgctcgggc cgaacccggt
      601 cgacgtcttt acccagtcga cgccgagctc gccgcaaatc ctgcaaagcg ttcgcttttg
      661 cccgtcgtcg aggtaggcat tctcgaagat cgacttgagc ttcttccccc gctggtgaca
      721 aagggtgacg agcgactcca cctcgcgtgc gaccgactgc cactccccgc tcaagacctg
      781 gctcacgttg acgagcgcat cgagctcttc agcgccgtcg tcgagcgcgg cctccgcttc
      841 ggcaagcttt gcgcgcgtgc tcgtcgcgcc atgtggaaag ccaatcgtcg tcgacgtgag
      901 tacgctggag cccgcaagtg cctcgcgtgc gcggcgcacg tagtaaggta aaatgcaaac
      961 gctcgcgacc tcgtacgacc gggcgagcgc aaggcccgat tcgagcgcgc tccggtcgag
     1021 gttcgggagc aacagcgcgt ggtcgatgag tttcgcaatc tccgcatggt cggcggcggt
     1081 cattccgggc atcttacctc ttttcgtgat cggatcccgg gccgcgtcac atgttgagga
     1141 acaagatgca acctttccgg ttctggcgta ggtcactcga aacgaacgct ttcggcgccg
     1201 cgctcgttct cgccctcggt tgcggcgcga ccgactcgac gaaatccccg gcggacggcc
     1261 aaaacgagct cgccgagcgc gatccggctg agctctacga ctgggaccac gtccccacct
     1321 tcgagctctc gctaccaccg ggtgagttcg agcacttgct cgcgacggcg gcggacgaag
     1381 tctatacccg ggcgagcctg accttcgatc aaaagccaat cggggacgtg gggctgcgat
     1441 tcaagggcag cgtcggcacc ctggagagct gcttcgacgc tcaggggaac ctgacttgcc
     1501 ccaagctcag catgaagctc gggttcgacg agtacgtccc gagcacgcgc ttctttcgac
     1561 agaagcggct caacctccac tcgatggtgc gcgacgatac caagctccac gagcgcctgg
     1621 cctacgacct ctaccgaagc atgggcatcg tggcgccgcg cagcgcgtgg gccgtggtca
     1681 aggtgaacgg ggagtcgctc gggctcttct cgatggtgga gcagatcgac gggcgcttca
     1741 ctgcgaatcg ttggcccggc gcgggcgacg gcaatttgta caaggaggcc tggcccctct
     1801 ccacggacgc acagtactac gaagaccacc tcgagaccgg agcctcacaa ccggacgccg
     1861 ctccgcacag cgcgtacgtc gactttacga gcgcgctctc ggccgggggc gacgacaaag
     1921 gccgcggcgc gctcggtcga tacatggacc tcgaatacct ctcgcgctac atggccgtgg
     1981 acgacgcgat cctcaacgtc gacggcgtca ccgcgacgta caccggcgat gacccacgct
     2041 actacgcgaa ccacaacttt tacgtctatc aagaagaaca gcgcggcttc ttctggctcg
     2101 tgccctggga catggacgcg acgttcaacc tgcgcggcga cttcgagctc gttccgcgtt
     2161 ggaacgggag cggcgccgac tgcacgagcc gctacaccgt ctggggcgca gcgcgcgtgg
     2221 tctcgcccgc ttgcgaccac ttgtttcaag cgctcgcgag cgacccgtcg agctaccagg
     2281 ccgccgtcga cgagcttctg gcaggggact tcagcgaagc gaagctgaac gagacgatag
     2341 atcgccacgc cgccttcatc gcgagcgcgg tcgagcacga tcccaagggc gccgggctga
     2401 gcgcgtggca aggcgccgtg agttacctga aacgacagct tccactcgtg cgagcgcacc
     2461 tcggtcgcgc aaagctcggc ttgtcgagca agccgctcat gctcgccgcg gaaagcgtga
     2521 ccgact
//
LOCUS       DASTKS010000512         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_81328,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000512 DASTKS010000000
VERSION     DASTKS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81328"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFQ35_10595"
     CDS             complement(<1..186)
                     /locus_tag="VFQ35_10595"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET9931128.1"
                     /translation="MHVRFSWDPAKAASNAAKHGVTFKEAITVFDDLGIHIELQRHAN
                     EIRLSATGYSSNHRLLVV"
     gene            complement(203..325)
                     /locus_tag="VFQ35_10600"
     CDS             complement(203..325)
                     /locus_tag="VFQ35_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931129.1"
                     /translation="MKRRRSRVKTEHNGAKNGGGYYGTRAEGAARCSGCVEHRK"
     gene            complement(330..797)
                     /locus_tag="VFQ35_10605"
     CDS             complement(330..797)
                     /locus_tag="VFQ35_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II TA system antitoxin MqsA family protein"
                     /protein_id="HET9931130.1"
                     /translation="MKRCAECQSTELRQGKVEERIEVGGISFIADLPALICGECGENF
                     VDGPDLERFDLAVASWLTSQGQRTPEAFRFARKAIGMRAADLAQLVGVTPETVSRWEN
                     GHGAIDVGVFALLGELVADRIEGKENTLRRLRALAGPVKAPKRAVRVELPKAG"
     gene            complement(810..1067)
                     /locus_tag="VFQ35_10610"
     CDS             complement(810..1067)
                     /locus_tag="VFQ35_10610"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HET9931131.1"
                     /translation="MSPAEALAEIRRAAIARRVRFSPHARQRMNQRFVRPPDVYCALE
                     SATACMATEQERWKVAGPDRDGDELTMVVKIEGDVVVITVF"
     gene            complement(1064..>2155)
                     /locus_tag="VFQ35_10615"
     CDS             complement(1064..>2155)
                     /locus_tag="VFQ35_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931132.1"
                     /translation="QLRPRTLAAYRATADKLVAWAERVKLRSADDLNRARLMSFREAL
                     IAEAKRISKKGAKRGVFVETGERRSPERVNSELRKVRTVLGYLRRLELLPRVTNDDLQ
                     DALKRLPVASERIQYLRPHECQKLLEAALRHDAATYTETREEHAGKGRQRIGTTPRYD
                     PIAPFVAFVLLTGCRFGEALALDWTQVELDALDHEGRKVGEVHLKGAATKTHKARTIG
                     LEVSPALHKLLSAVHLASGGKGSVFALSRATAEAAAKRLRAEYGAPASFTWQALRRTC
                     GTYLTNAPGIFGAASAYRSAKQLGHSVQVAERHYVDVARGIPRDARTLEAAMQIDDAM
                     AKLLATASDRAVGGIADLKKTQVAGRIGL"
BASE COUNT          326 a          754 c          665 g          410 t
ORIGIN      
        1 gaccacgagg agacggtggt tgctcgagta cccagttgcg ctcaggcgga tctcgttcgc
       61 atgacgctgc agctcgatgt gaattcctag gtcgtcaaac acagtgatcg cttccttgaa
      121 ggtcactcca tgcttggcgg cgttgctcgc cgcttttgcc ggatcccaag agaagcgaac
      181 atgcatttct gaagtagtat gactactttc tatgctcgac gcaaccgcta cagcgggccg
      241 cgccctccgc gcgcgtgccg tagtagccgc cgccgttctt cgcgccgttg tgctcggtct
      301 tcacgcgcga tcgtcgcctc ttcatccggc tatccagcct tcggcagctc gacgcgcacc
      361 gcacgctttg gggcctttac agggccggcg agcgcgcgca gccggcgcag cgtgttctct
      421 ttgccctcaa tacgatcggc tacgagctcg ccaagcagcg caaagacacc gacgtcgatt
      481 gcgccgtgtc cgttctccca acgcgagacg gtctcaggcg tgaccccgac gagctgcgca
      541 agatccgcgg cgcgcatgcc gatcgccttg cgtgcgaagc gaaaggcctc cggcgtccgc
      601 tgcccttgac tcgtgagcca gctcgccacg gcgaggtcga agcgctcgag gtccggaccg
      661 tccacgaagt tctcgccgca ctcgccacag atgagcgccg gcaggtccgc aatgaagctg
      721 atacctccca cttcgatgcg ttcctccacc ttgccctgtc gtagctcggt gctctggcac
      781 tcggcacagc gtttcatagg ggctcctcat caaaagacgg tgatcacgac aacatcgccc
      841 tcgatcttca cgaccatcgt gagctcgtcc ccgtcccggt ccgggccggc caccttccat
      901 cgttcctgct cagtggccat gcaagcagtc gcgctctcca acgcgcaata gacatccgga
      961 gggcgcacga accgctggtt catcctctgc ctcgcgtgtg gcgagaagcg aacgcgccga
     1021 gcgatggcag ctcgccggat ctcggcaagg gcctccgccg gactcatagc cctatcctgc
     1081 ccgcgacttg agtttttttc aagtcggcga ttccccccac ggctcgatcc gacgcggtag
     1141 cgaggagctt cgccattgcg tcgtcgatct gcatcgcagc ttcgagcgtg cgcgcgtctc
     1201 gcggaatccc gcgcgccacg tccacgtaat gccgctccgc cacctgcacg ctgtgtccca
     1261 gctgctttgc gctgcggtac gcgctcgctg caccgaagat gcccggcgcg ttcgtaaggt
     1321 aggtaccgca ggtgcggcgc agcgcctgcc acgtgaagct cgcgggcgcg ccgtactcgg
     1381 cacgcaggcg cttcgctgca gcctcggcgg tcgctcgcga gagcgcgaac acgctgcctt
     1441 ttccgccgct cgcgaggtgc accgccgaga gcagcttgtg gagcgcgggc gagacctcga
     1501 gcccgatcgt gcgcgccttg tgcgtcttcg tcgcggcgcc cttcaggtgg acctcgccga
     1561 ccttccggcc ctcgtggtcg agcgcgtcca gctccacctg tgtccagtcg agcgcgagcg
     1621 cttcgccgaa ccgacagccg gtcagcagca cgaacgcgac gaacggcgca atcggatcgt
     1681 agcgcggcgt cgtgccgatc cgctggcggc ctttgcccgc gtgttcttcg cgggtctcgg
     1741 tgtatgtcgc cgcgtcgtgt cgcaaggcgg cctccaagag cttctggcat tcatgcgggc
     1801 gcaggtattg gatgcgctcg gaggcgaccg gcaaacgctt cagcgcgtct tgcaggtcgt
     1861 cgttggtcac tcgcggaagc agctcgagac gtcgcaggta gccgagcacc gtgcgcacct
     1921 tgcgcagctc gctgttcacg cgttcgggcg atcggcgctc gcccgtttcg acgaagaccc
     1981 ctcgcttggc gccctttttc gagattcgct tcgcctccgc gatgagcgcc tcgcgaaagc
     2041 tcatcagccg tgcgcggttg aggtcatcgg ccgagcgcag cttcacccgc tcggcccaag
     2101 cgacgagctt gtcggccgtt gcccgataag cggcgagcgt tcgcggccgg agctg
//
LOCUS       DASTKS010000513        12987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_81446,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000513 DASTKS010000000
VERSION     DASTKS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12987
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81446"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VFQ35_10620"
     CDS             complement(<1..1357)
                     /locus_tag="VFQ35_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931133.1"
                     /translation="MCAATVVYAGRASAYDARVDASFDAQYYSLSSPFGDPYIYRRRY
                     TSTLGLDLDDIQGERTRRGPRLNFRSRLRVDGDFAQDYAERDPASNRYVPGLAQSPLD
                     VMFAYVDGRGFVNGLFNFRLGRQYLIDALGYWSFDGALVEAMLPVHVALSGFLGFEQR
                     TGLPMLAGARFSGDGVWRGDRSGLEQNQYPAFLDDSSLAPARGVALETWGFSRFHSRL
                     SYRRVDNQSPVLVSAFFDPKEPLRFVKTTRTSSERVGYSLRIDAGDVGSLSARATYDL
                     YNRAVSDALVSVDVYAPKNLIVGADVDYYYPTFDADSIFNFFTHRGMTTAVARLSYAS
                     KSALSGTASAGLRWYATSGDPQVFGEQQLNDTTSRATAVQRDWIAQGGARYRMGPSTI
                     AFDLNAQSGISGHRVGGDLSTRRTFQGGRVDALAVASLYDWNDPVRPTRSATCFTYVL
                     GA"
     gene            complement(1469..3775)
                     /gene="pilQ"
                     /locus_tag="VFQ35_10625"
     CDS             complement(1469..3775)
                     /gene="pilQ"
                     /locus_tag="VFQ35_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET9931134.1"
                     /translation="MSFALQRQIGAGRLVGSALALLLALSVTARAGAESPSLRHAQKM
                     TVAADSDGGAVLRVDFSDVPAFTARLEQNGMRLVVDVPNADLQGIPAALTDRVGVVGG
                     VLTQAFKAGNQSTARILVTLLKSARYAVTVEGTTLLIRLAPGGSGVLDSPVKPATPKV
                     AAAVDPRVANVRFEHSELEDRVLIDMSESAEFDEPVFDGERRRLVLNAKLPSELERKY
                     DVSAFNGRVSAVSSYVGTGGRVVLEADCKRGVETRVSRRGTTLVWSFVEPGKLPATVT
                     GVARDGKAARRGRTVFVEPAPTGITEVHTGYENDVVETHSAGDQAAAFAPNLLGQQKK
                     AFGGRRIDLDLKDADIHNVLRLLADVGQVNIITADNVSGTVTIRMRNVPWDQALDVVL
                     QSKNLGMVRQANMIRVAPLADLEKEREMQLARRLQEVKLAPIETRLIPISYAQADDLQ
                     ERARGMLSERGSIAVDERTNVLIVRDIAGNLNQIEELLRSLDTQTPQVLIEARIVEAS
                     SRYLRDVGIQWGGDATFSGATGNPTGLAFPNSIGVTGGASDGQTPTAGLSPFTNSVAN
                     PNFAVNLPAAVGTGQGGAIGLTFGSIDNNFNLALRLSAAEASGMLRILSSPRVLTLDQ
                     KQARIAQGTLIPFSQVSAQGVQTTFQEAKLQLLVRPHVTADGSVSMHVKINRDEPDFN
                     QTSARGDPTILKREAETDLLVMDGHTAVIGGIYTRNTGRNLDQVPFFGDIPLLGLLFQ
                     RRRTSDQRGELVIFITPRIVNRAEALGR"
     gene            3939..4997
                     /locus_tag="VFQ35_10630"
     CDS             3939..4997
                     /locus_tag="VFQ35_10630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9931135.1"
                     /translation="MTTAHRLLNRMVGKELPGAQYLVTNADAPQLEFSVGMRDAASGQ
                     PMSPETLLMAYSMTKAITAVALVQLVDAGKVGIDEALSAFFPDHPYGREVTLRMLLAQ
                     TAGIPNPMPLDWFEVEGKSFDREEKLRNLLSKYPRLKHRAGHAYSYSNLSYWLLEKVV
                     EAASKADFADYVRANVFGRLGILGGSATFTLPPAATLATGHARRYSLQTLFVRWLSPR
                     DYWVEPSGSFCRTARVTPHGRGYGGLFTNAGALGAVLRDLLREEPKLMSRSAKALMFS
                     QQRANGRPIDMTLGWVIGRCQGTTYFGKQGGGLGFNGNLRIYPERGVATVFLANGTEL
                     SPGPIDRRSDVLDAAFLK"
     gene            complement(4999..5586)
                     /locus_tag="VFQ35_10635"
     CDS             complement(4999..5586)
                     /locus_tag="VFQ35_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET9931136.1"
                     /translation="MSRRSWTLGCLAAAALVLAACEDKMMVDEGAPPAPTPTEAVAAA
                     PKPAAVAPPRVRIEESDFVESDRNRDPFRSFVRSFVEDKTGERRNQREVVLSDYSLDE
                     LKLIGIVTRMQPAKAMLVDPTGKGHVIQRGQFVGRPDVVQGTGRGGSTYEVNWRVDSI
                     RDGDVVLVRSDPANPDVPTATRVIPLRPEGTRVER"
     gene            complement(5583..6209)
                     /gene="pilO"
                     /locus_tag="VFQ35_10640"
     CDS             complement(5583..6209)
                     /gene="pilO"
                     /locus_tag="VFQ35_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET9931137.1"
                     /translation="MAMARADLSLGKLPMGARVAVGALMMALVGVAYYVVFFSELAGR
                     IAGEKAREGQLTSELSEVRKAEFAYQKDLTELTEREQRQRELNKILPTTSEYPAFLSA
                     VQNVANVSGVALTAWSPNPEVPEKFYARIPMRVELSGRFHQIEKFFYGVGQLDRVINI
                     ENISLTDPKVVGEDLVLKAEGLATAFRALDNASAPGGDKRGSQMGGAP"
     gene            complement(6209..6850)
                     /locus_tag="VFQ35_10645"
     CDS             complement(6209..6850)
                     /locus_tag="VFQ35_10645"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET9931138.1"
                     /translation="MIRINLLSRKKRTAEAPQKTQVWLLVALGCVLVEVVVLVILHGL
                     KTDELRAQERRNSEVSAQIEQSKRAVQSHDAVKQKLALLRAREEAIAKLESARSGPTS
                     VLVELSRILTPGRGPSVDASKITQIRRDDPGAAFNPNWDTRRLWLTSFREEKRRMHVD
                     GLARDGEDVSEFARRLSLSDFFQGVKLLPAHRQVDAATKVDLVQFSLEAEVKY"
     gene            complement(6847..7917)
                     /gene="pilM"
                     /locus_tag="VFQ35_10650"
     CDS             complement(6847..7917)
                     /gene="pilM"
                     /locus_tag="VFQ35_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9931139.1"
                     /translation="MGEGKNLVGVDIGSGSIKVCLVKESRRGLGLVRLGYVPLSPQVI
                     VDGQVMDSGAVVEALGRAFSEAKIRQKECALSVSGQSVIIRKITVPMMTPAELDEQIQ
                     WEAEQHIPFDIKDVQVDYQVLRKRPDVSQMDLLLVAAKRDRIEDYAQLARNAKLKPLV
                     CDVDSFTVQNLFEYSRGLPDNQTIALINVGASFSSLSIIANGVHVFPREIANGGNAIT
                     EEIQKQLGIPYEQAEAYKCGGSQGDPNGGMVPQQVVQIVEAASDSIAAEIQRSIDFYL
                     ATSGEGEIARIYVTGGTANLAALARAIERRARVPVEIWSPTEKISIEAKEVDAQLLMR
                     RSAQLSVALGLSLRKEKEVRLG"
     gene            8330..9184
                     /locus_tag="VFQ35_10655"
     CDS             8330..9184
                     /locus_tag="VFQ35_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931140.1"
                     /translation="MPSTTAARAAQDSETREARPSVAPRPSVAPRPSVFPRAGGSKVK
                     AAPAARRHNGTATANRKARRPAAPKSPATPVALRPVERHVENSARARYLLEEYTPLVR
                     KIAGGFQRKLPRNVLREDLMAAGMSGLWDAIRRHGDTAGETFEWYVRVRVRGAILDEL
                     RAQDWLPRRARAAAASASAESGEYVPPPAVVRLDDVSEWEQSRCLSDLTSSETAVAAK
                     FTHETLAKAVQLLPERERHIVSEHYFRGVKFKDLGAELGVSEPRISQLHSRAMRRLKT
                     LMAEVQTA"
     gene            complement(9602..10930)
                     /locus_tag="VFQ35_10660"
     CDS             complement(9602..10930)
                     /locus_tag="VFQ35_10660"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9931141.1"
                     /translation="MNDVNPPPSSAAPSVGGSSVIPLPPRYEFFKVIQNYLDQAGKLA
                     GIPEHVALILSQPKAEIIVNFPVRMDDGKFRVFKGYRIQHNNILGPYKGGMRYHQDAS
                     LDDFKALAAMMTWKCALMGLPYGGGKGGIKFNPRDVSRGELSRITRRFFHALGTNIGP
                     DYDIPAPDVGTNAQTMAWALDTYANTVGTVHKTAMQGVVTGKPLSCGGTVGREKATGQ
                     GLVHSVRLWAERNKFDLSGKKLILQGFGNVGSNAAVLLSHLGVSLVAVGDHTGYFYNS
                     EGFNAYRLKQYVEQNGSISGYPNGSRISREEFFGIQADLFVPAALENQVGPQEAEALQ
                     VRLVAEGANGPCSPEGEEVLRRRNIEILPDVLANAGGVTVSYYEWVQNKRSETWTLEE
                     VDERLERAMVDAYERVEHFAETHHCDFRLACYGVALERLARAYVDREIFP"
     gene            complement(11172..12014)
                     /locus_tag="VFQ35_10665"
     CDS             complement(11172..12014)
                     /locus_tag="VFQ35_10665"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9931142.1"
                     /translation="MVRSNLALRLLTAAVVVPPLLLLLFRGPAWGFYALVLLVAVLGA
                     FELFRMTHPGDAISQGIGVAFTAAVSLALYWRPNDARVLLTILLLVPTLGIMVPLVRL
                     GTIATAGIRIMAGVAGPLYVGASLTTLALLKSELGPSGPAWVVLALTFAWLADTGGYF
                     FGRFLGKTKLYEAVSPKKTRAGFVGALVGAACGALAARFWFLPEINLAEVLPLAVVAG
                     ALGQFGDLVESLLKRSTGIKDSGAVVPGHGGILDRIDALLVVAPLVYLYVTWSGAAQH
                     LQGN"
     gene            12165..>12987
                     /locus_tag="VFQ35_10670"
     CDS             12165..>12987
                     /locus_tag="VFQ35_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931143.1"
                     /translation="MLGSPVGYLRRRGVALALAGSAALAARCASDGPPARTLDGLAQL
                     LGNAAHGVVEPEAIVWEPSAGLLVDTFVGRRVLFLASAKAGEPRDVYRARVRLALDGA
                     PIHVSDIKNLTDTPHGDDVALEGDGERVSFATLAFGRIQAISVLETTGVRDADRPSGA
                     LNRALFALSSFDKTGSLAGVGRTDIVLDVPARSARLTMDAAHLVVDFGERGRGLSYDL
                     ERRTVRGTDGGEAYAARVVPEAYGAKPPLLWAVDTVRGVVGPRPIAWLENVVFGAK"
BASE COUNT         2270 a         4337 c         4134 g         2246 t
ORIGIN      
        1 ccgcgccgag cacgtaggtg aagcacgtgg ccgagcgagt cggtcggacc ggatcgttcc
       61 agtcgtaaag ggacgctacc gccagcgcat ccacgcgacc gccttggaac gtgcggcgag
      121 tactgaggtc gccgccgacg cgatgcccgg agatcccgct ttgcgcgttc agatcaaacg
      181 cgatggtgct cggtcccatg cgataacgtg cgccgccctg cgcgatccaa tcgcgctgaa
      241 cggcggtcgc gcgcgacgtc gtatcgttca gttgctgctc gccgaagacc tgcggatcac
      301 ccgaggtcgc gtaccagcgc agccccgcgg aggcggtgcc cgagagcgcg ctcttcgatg
      361 cataactcaa gcgcgcgacc gccgtcgtca tgccgcgatg ggtaaagaaa ttgaaaatcg
      421 aatcggcgtc gaacgtcggg tagtagtagt cgacgtcggc cccgacgatg aggttcttcg
      481 gtgcgtacac gtcgaccgac acgagcgcgt cgctcacggc ccggttgtac aagtcgtaag
      541 ttgcgcgcgc cgagagggaa cctacgtcgc ccgcgtcgat acgcaacgaa tatccgacgc
      601 gctcactcga ggtgcgcgtc gtcttgacga agcggagcgg ctccttggga tcgaagaacg
      661 ccgacaccag caccgggctt tgattgtcga cgcgacgata cgacaagcgc gaatgaaatc
      721 gcgaaaagcc ccaggtttcg agggcaaccc cgcgagctgg ggccagcgat gaatcgtcga
      781 gaaacgcggg gtactggttc tgctcgaggc cgctgcggtc accgcgccac acgccgtcgc
      841 cactgaagcg cgcccccgcg agcatcggga gccccgtgcg ttgttcgaac cccaagaacc
      901 cggagagagc gacgtgcacc ggtagcatcg cctcgacgag cgcgccgtcg aacgaccagt
      961 agccgagcgc gtcgatcagg tactgccgac cgagccgaaa gttgaacagc ccgttcacga
     1021 acccacgccc atcgacgtag gcgaacatca cgtcgagcgg cgattgtgcg agccccggca
     1081 cgtagcggtt cgacgccgga tcgcgctccg cgtagtcttg ggcgaagtcc ccgtcgaccc
     1141 gcaggcgcga gcgaaagttc agccgcggtc cgcgacgcgt ccgctctcct tgaatgtcgt
     1201 cgagatcgag cccgagcgtc gacgtgtagc gacgtcggta gatgtacgga tcgccgaacg
     1261 gactcgacag cgaatagtac tgggcgtcga agcttgcatc gacgcgcgcg tcgtacgcgg
     1321 acgcccgccc cgcgtacacc acggtcgcgg cgcacacgcc gagccagcac ccccgccgtg
     1381 ctcgctccgt cgccgctaca cctcgcattt cctcgagcga gaatcgtcgc cgcgccgggc
     1441 gctgtcaatc gcgcggcgcg gccctgcctc agcgaccgag cgcctcggct cggttcacga
     1501 tgcgcggggt gatgaagatg acgagctctc cccgctggtc gctggttcgc cggcgctgga
     1561 agagcaggcc caacagcgga atatccccga agaacgggac ttgatcgaga ttacggcccg
     1621 tattgcgcgt atagatcccg ccgatcaccg cggtgtgccc gtccatcacg aggaggtcgg
     1681 tctcggcctc gcgcttcaag atcgttgggt cgccgcgcgc cgaggtctga ttgaagtcgg
     1741 gctcgtcgcg gttgatcttc acgtgcatgc tgacactgcc gtcagcggtc acgtgcggcc
     1801 gtaccaagag ttgaagcttg gcctcttgga aagtcgtctg cacgccttgc gcgctgacct
     1861 gcgagaacgg aatcaaagtg ccttgcgcga tgcgcgcttg cttttgatcc agcgtcaaca
     1921 cgcgcgggct gctcaggatg cggagcatgc cgctcgcctc ggccgccgac agacgcagcg
     1981 cgaggttgaa gttgttgtcg atcgatccga aggtaagacc gatcgcgcca ccttgaccgg
     2041 tgccgaccgc ggccggcagg ttcaccgcga agttcgggtt cgcgacgctg ttcgtgaagg
     2101 gactcaagcc ggccgtcggc gtttggccgt cgctcgcgcc accggtgacg ccgatcgagt
     2161 tcgggaaggc gagccccgtc gggttgccgg tcgcgcccga gaaggtggcg tcgccgcccc
     2221 actgaatgcc gacgtcgcgc agatagcggc tcgaagcttc tacgatgcgc gcctcgatga
     2281 gcacctgggg agtctgcgtg tcgagcgaac gcaggagctc ctcgatttgg ttcaggttgc
     2341 cggcgatgtc acgcacgatg agcacgttcg ttcgctcgtc gaccgcgatc gacccgcgct
     2401 ccgagagcat tccgcgagcg cgctcttgta gatcgtcggc ttgcgcgtag ctgatcggaa
     2461 tcaggcgcgt ctcgatgggc gcgagcttga cctcttgcaa gcgccgggca agctgcatct
     2521 cgcgctcttt ctcgaggtcg gcgagcggag ccactcggat catgttcgct tggcgcacca
     2581 tgccgaggtt cttcgactgc agcacgacgt cgagggcctg gtcccagggc acgttgcgca
     2641 tgcggatggt gaccgtgccg ctcacgttgt cggcggtgat gatgttcacc tgaccgacgt
     2701 cggcgagcag gcgcagcacg ttgtggatgt cggcgtcttt caggtccaga tcgatgcgcc
     2761 ggccaccgaa tgcttttttc tgctgaccga gcaggttcgg cgcaaacgcc gccgcttgat
     2821 cacctgcgct gtgcgtctcg accacgtcgt tctcgtagcc agtgtgcacc tcggtgatgc
     2881 cagtgggcgc tggctcgacg aacacggttc gaccccggcg cgcggctttt ccgtcgcgcg
     2941 cgacgccggt cacggtggcg gggagcttac cgggctcgac gaaagaccag accagagtcg
     3001 tgccgcgccg gctcacgcga gtctcgacgc cgcgcttgca gtcggcctcg agcacgacgc
     3061 gcccgccggt gcccacgtag ctcgacaccg cgctcacacg cccgttgaaa gcggaaacat
     3121 cgtacttgcg ctcgagctcg gaaggcagct tcgcgttgag caccaagcgc cgtcgctctc
     3181 cgtcgaacac gggctcgtcg aactcggcgc tctcgctcat gtcgatgagc acgcggtcct
     3241 cgagctcgct gtgttcgaac cgcacgttgg cgacgcgcgg atctaccgcc gcggctacct
     3301 tcggcgtggc cggcttcacc ggcgagtcca aaacccccga accgcccgga gcgagccgga
     3361 tgagaagagt ggttccctcg accgtcaccg cgtaacgggc gctcttcagc aaggtcacca
     3421 gaattcgcgc cgtgctttgg ttcccggcct tgaaagcctg ggtcaacacg cccccaacga
     3481 ccccgactcg atccgtcagg gctgcgggaa tgccttgcag gtcggcgttc gggacgtcca
     3541 cgacgagccg catcccgttc tgttcgagcc gcgccgtgaa ggccggaacg tcgctgaaat
     3601 cgacccgcag cacggctccc ccatcggagt cggccgctac cgtcatcttc tgcgcgtggc
     3661 gcagactcgg cgattcggct cccgcacgag cggttaccga aagcgcgagc agcagcgcga
     3721 gtgccgagcc aacgagccgc ccggctccaa tctgacgctg tagagcaaaa ctcataacct
     3781 gagctccaaa agcccgcaaa tctaccgact gggactcggc agccagccta gcgtttcgct
     3841 tccacgagga gcaaaaaaag cgagacccac ctggcgctta atgaagcgcg ccgatacgcc
     3901 ctcgcccccg cgctcgccgc catgttacgg agctcgaaat gacgaccgcc caccgactcc
     3961 tgaatcgaat ggtgggaaaa gagctacccg gcgcgcagta cctcgtcacg aacgccgacg
     4021 cgccacagct cgagttctcg gttggaatgc gcgacgccgc cagcggccaa cccatgtcgc
     4081 cggagacgct tctgatggcg tattcgatga ccaaggcgat cacggccgtc gcgctcgtcc
     4141 agctcgtcga cgcgggcaaa gtgggcatcg acgaggcgct cagcgccttc tttccggatc
     4201 atccgtatgg gcgcgaagtc acactgcgaa tgctgctcgc gcagaccgcc ggcatcccca
     4261 acccgatgcc gctcgattgg ttcgaagtcg aaggaaagag cttcgatcgc gaggaaaagc
     4321 tccgaaacct gctctcgaag taccctcgac tgaagcatcg ggccgggcac gcctattcgt
     4381 attcgaactt gtcgtactgg ctgctcgaga aggtcgtcga agccgcatcg aaggccgact
     4441 tcgcggacta cgtgcgcgcc aatgtcttcg ggcggcttgg gattttgggg gggagtgcga
     4501 cgttcacgtt gccgcctgcc gcgactctcg cgacggggca tgctcgtcgc tactcacttc
     4561 agaccttgtt cgtacgctgg ctttcccccc gtgattactg ggtcgaaccg agcggctcgt
     4621 tttgccgaac cgcgcgcgtc acgccccacg gtcgcggcta cggagggctc ttcaccaacg
     4681 ccggcgcgct cggcgcggtg ttgcgcgatc tcttgcggga ggagccaaag ctgatgagcc
     4741 gctcggcgaa agcgttgatg ttcagccagc aacgggcgaa tgggcgtccc atcgacatga
     4801 cgctcggctg ggtcatcggc cgttgccagg gcacgacgta cttcggcaaa caaggcggag
     4861 gcctcggctt taacggcaac ctcaggattt atccggagcg cggcgtcgcg accgtgttcc
     4921 tcgcgaacgg caccgagcta tcacccggcc ccatcgatcg acgcagcgac gtgctcgacg
     4981 cggccttctt gaaatgactc aacgctcgac gcgcgtacct tcgggacgca gcgggatcac
     5041 gcgcgtcgcc gtcggcacgt cggggttcgc cggatccgag cgaaccagca cgacgtcgcc
     5101 gtcgcggatg ctgtccacgc gccagttcac ttcgtacgtc gagcctccgc gcccggtccc
     5161 ctgaacgacg tcggggcgac ccacgaattg gccgcgctgg atgacgtgcc ccttccccgt
     5221 cggatcgacg agcatcgcct tggccggctg catgcgcgtc acgataccga tgagcttgag
     5281 ctcgtcgagc gagtagtcgg acagcacgac ctcgcgttgg ttgcgccttt cgccggtctt
     5341 gtcctcgacg aaagagcgca cgaacgagcg aaacggatcg cgatttcggt cgctctcgac
     5401 gaagtcggac tcttcgatgc gcacgcgcgg cggagcgacg gccgcaggct tcggggccgc
     5461 cgcaaccgct tcggtcggag tcggcgcggg cggagccccc tcgtcgacca tcatcttgtc
     5521 ctcgcacgcg gcgaggacca acgccgcggc cgcgaggcaa ccgagcgtcc aagagcgccg
     5581 actcatggag cgcctcccat ctgtgaacct cgcttgtcgc ctccgggggc gcttgcgttg
     5641 tcgagcgcgc ggaatgcggt cgcgaggccc tccgccttca acaccaggtc ttcgcctacc
     5701 actttggggt cggtgagcga gatgttctcg atgttgatga cgcggtcgag ctgccccacg
     5761 ccgtaaaaga atttctcgat ttgatggaag cgcccggaga gctccacgcg catagggatg
     5821 cgtgcgtaga acttctcggg gacctccggg ttcggtgacc aggccgtgag cgcgacgccg
     5881 gagacgttcg cgacgttctg caccgcgctc aagaacgctg ggtactcgct ggtggtcgga
     5941 aggattttgt tcagctctcg ctggcgctgc tcgcgctcgg tgagctcggt gagatccttt
     6001 tgatacgcaa attcggcttt gcgaacctcc gacagctcgg aggtcagctg cccttcgcgc
     6061 gccttctctc ccgcaattcg acccgcgagc tcgctgaaga aaacgacgta gtaagcgacg
     6121 ccgacgaggg ccatcatcag ggcgcccacc gcgacacgcg cccccatcgg gagcttgccg
     6181 aggctcaggt cggcacgagc catagccatc agtacttgac ctccgcctcg agggagaact
     6241 gcacgaggtc caccttggtc gcggcgtcca cctggcgatg cgcgggcagc aacttgactc
     6301 cctggaaaaa atccgagagc gagagccgcc gcgcgaattc gctcacgtct tccccatcgc
     6361 gcgccagccc gtcgacgtgc atgcgccgct tctcttcgcg gaaactcgtc agccagaggc
     6421 gtcgcgtatc ccagttcggg ttgaacgccg cgcccggatc gtcacggcgg atctgcgtga
     6481 tcttcgaggc gtccacgctc gggccccggc ccggggtgag aatgcgcgaa agctcgacca
     6541 gcaccgaagt ggggccgctg cgcgcgctct cgagcttcgc gatggcctct tcgcgggccc
     6601 tgagcagcgc gagcttttgt ttcactgcgt cgtggctctg cacggcgcgt ttgctctgct
     6661 cgatctgcgc cgacacttcg ctgttcctgc gctcctgagc ccgcagctcg tccgtcttca
     6721 agccgtgtaa aatcaccagc acgaccacct cgacgagcac gcagccgagc gcgacgagca
     6781 gccagacctg cgtcttctga ggtgcctcgg cggtgcgctt cttgcgcgag agtaagttga
     6841 tgcggatcat ccgagccgca cttctttctc tttgcgcagg ctgagaccca gcgcgaccga
     6901 tagctgcgcc gaacgccgca tcaagagctg cgcatcgacc tctttggctt cgatgctgat
     6961 cttctccgtc ggagaccaga tctcgactgg cacgcgcgcg cgccgctcga tggctcgcgc
     7021 caacgcagcg aggttcgcgg tcccaccggt cacgtaaatt ctggcgattt cgccctcacc
     7081 gctcgtggcg agataaaaat cgatgcttcg ctgaatctcg gccgcgatcg agtcgctcgc
     7141 cgcctcgacg atttgcacga cctgctgtgg caccatcccg ccgttcgggt cgccttggct
     7201 gccgccgcac ttgtaggcct ccgcctgctc gtacgggatc ccgagctgct tctgtatttc
     7261 ttcggtgatc gcgttgccgc cgttcgcgat ttcacgaggg aaaacgtgca cgccgttcgc
     7321 gatgatgctc agcgacgaga agctcgctcc cacgttgatg agcgcgatcg tctgattgtc
     7381 gggcagtcct cgcgaatact caaataggtt ctgcaccgtg aacgagtcca cgtcgcacac
     7441 gagcggcttc agcttggcgt tccgtgcgag ctgcgcataa tcctcgatgc gatcccgctt
     7501 cgccgcgacg agcaggagat ccatctgact tacatcgggt cgcttgcgga gcacctggta
     7561 gtcgacctgc acatctttga tgtcgaaggg gatgtgctgc tcggcctccc actgaatctg
     7621 ctcgtcgagc tcggccggcg tcatcatggg aaccgtgatc ttgcggatga tgacgctctg
     7681 ccctgacacg ctcagggcgc actctttctg gcggatcttg gcctccgaaa aggcgcgccc
     7741 caaggcctcg accaccgcgc cggagtccat cacctgccca tccacgatga cctgcgggga
     7801 gaggggcacg tacccgagcc gcaccaggcc caggccgcgt cgcgactctt tcaccaggca
     7861 gaccttgata gagcccgacc cgatgtcgac gccgactagg ttctttcctt cgcccatgga
     7921 aacgtaccgg cggaaaaagc cgccggtgaa aactttggca tccgccccag tcaatagcca
     7981 aaaaaggcgt agcgattact ggactgagtg cctgaaacgg ccttgttgca gggtagttaa
     8041 ggctcgcctg ggtcgcgctc gaccttccgc aagaacagga gaaaagcgaa agggggactt
     8101 aagtctgcgt cagggggcag gcgctgggtc gtcccaaacc caccgcgtgt ctcgggtatg
     8161 gaaaggcggg catcgcgaaa acccgagact cgtgggacgg cgccgaaaca cgcactgggt
     8221 tgggagcgac ccaggagggc tacctctttt cgactaacca ttcgtaatac ttcaataaaa
     8281 tacatcaggc acgcgcgttg cagaaggttg cgcctggaga ccgcgactaa tgccttcgac
     8341 caccgcagcc cgagccgccc aggattccga aacacgcgaa gcacgtccaa gcgttgcccc
     8401 gcgccccagc gttgccccgc gccctagcgt cttcccgcgt gcgggaggtt ccaaggtcaa
     8461 agcggcgcca gcagcccgtc gccacaacgg caccgccacg gcgaatcgta aggcgcgtcg
     8521 ccccgcggcg cccaagtcac cggcgactcc cgttgcgcta cggccagtcg agcgccacgt
     8581 cgaaaactcg gcgcgcgccc gctacctcct cgaggagtac acgccgctcg tccgcaagat
     8641 cgccggaggc ttccagcgca agcttccccg caacgtactc cgcgaagacc tcatggcagc
     8701 aggcatgagc ggcctttggg acgcgatccg tcgtcacggc gatacggcgg gcgaaacctt
     8761 cgagtggtac gttcgcgtgc gcgttcgcgg cgccatcctc gacgagctcc gcgcacagga
     8821 ttggttgccc cgccgcgctc gcgccgcagc tgcttcggct tccgcggaga gcggcgagta
     8881 cgtgccgccg cccgcagtcg tgcgcctcga cgacgtcagc gagtgggagc agtcgcgctg
     8941 cctcagcgac ctcacgtcca gcgagacggc cgttgccgcc aagttcacgc acgagaccct
     9001 cgcgaaggcc gtacagctct tgccggagcg cgagcgtcac atcgtgtccg agcactactt
     9061 ccgcggcgta aagttcaagg atctcggtgc cgagctcggc gtcagcgagc cgcgcatctc
     9121 ccaacttcat tctcgcgcca tgcgtcgctt gaagacgctc atggccgagg ttcaaaccgc
     9181 ctgagttccc ctcgctcctc aggcggaccg aacgccgaac ggatgtctcc ccgttcggcg
     9241 ttcggctttt gtagtccccg gacacgccct tcccccggtc ccgggacgag tccagcccca
     9301 ccggtcccgg gacaagtcca gccccaccgg ttccgggaca agtcccgacg ccaccggtcc
     9361 tgggacaagt ccagccccac cggtcccggg ccaagtccag ccccaccggt cctgggacaa
     9421 gtgctgacgc caccggtcct gggacaagtc ccgatgcacc ggtcccggga caagtcccga
     9481 cgcaccggtc ccgggacaag tcccgacaca ccggtcccgg gacaagtccc gacgcaccgg
     9541 tcccgggacg agtcccgacg ccaccggtcc cgggacaagg caggcctccc ctggccgggg
     9601 gctacggaaa gatttcgcgg tcgacgtacg ctcttgccag acgctcgagc gcgacgccgt
     9661 agcaggcgag gcggaagtcg cagtgatgtg tctcggcgaa gtgttcgacg cgttcgtacg
     9721 catcgaccat tgcgcgctcg aggcgctcgt ccacctcttc gagggtccaa gtctcgctgc
     9781 gcttgttctg tacccactcg tagtagctca ccgtgacgcc accggcgttc gcgaggacgt
     9841 cagggagaat ctcgatgttg cggcggcgca gcacttcttc accttcgggc gagcacgggc
     9901 cgttcgcacc ttcggcgact agccgcacct gcaatgcttc cgcttcttga gggccgacct
     9961 ggttttcgag tgcggcagga acgaacaggt ccgcctggat tccgaagaac tcctctcgag
    10021 agatccggct gccgttcggg tagccgctga tgctgccgtt ttgctcgacg tattgcttca
    10081 agcgataggc gttgaagccc tcggagttgt aaaagtaccc ggtgtggtcg ccgacggcga
    10141 cgagcgaaac tccgaggtgg ctgaggagca ccgccgcgtt cgagccgacg ttcccgaacc
    10201 cttgcaagat gagcttcttg ccggagaggt cgaacttgtt gcgctcggcc cataagcgaa
    10261 cagagtgaac caagccctga ccggttgcct tctcgcgacc aaccgtgcca ccgcagctga
    10321 ggggcttgcc cgtgacgaca ccctgcatcg cggtcttgtg gacggtacca acggtgttcg
    10381 cgtaggtgtc gagcgcccac gccatcgtct gtgcgttggt gccgacgtcg ggcgccggaa
    10441 tgtcgtagtc agggccgatg ttggttccga gcgcgtgaaa gaagcggcgc gtgatgcggc
    10501 tgagctcgcc gcgcgacacg tcacgcgggt tgaacttgat gccgccctta ccgcctccgt
    10561 agggaaggcc catgagcgcg cacttccacg tcatcatggc cgcaagcgct ttgaagtcgt
    10621 cgagtgacgc gtcctggtga taacgcatgc cacccttgta cgggccgagg atgttgttgt
    10681 gctggatccg atatcccttg aagacgcgga acttgccgtc gtccatgcgg acgggaaagt
    10741 tcacgatgat ctcggccttt ggctggctga ggatcagggc gacgtgttcg ggaatgcccg
    10801 ccagctttcc agcctggtcg aggtagttct ggatgacctt gaagaactcg tagcgcgggg
    10861 gaagcgggat cacggacgac ccgcccacac tcggcgcggc agagctggga ggagggttca
    10921 catcgttcat cggcgttctc cggggggcct cgatgctacc agcggccacc gaaactaccg
    10981 actaaatggc gccgcccctc ggcgatcggt agtccgttcg tggaccgacc aaaacagcgg
    11041 tgaccggacg caatcggcag tccctccctg gccggacccc cggacgcgag atcgcgcgtt
    11101 ttcccgcggg tgttcgcatc gagatgtcat gtgagctcac ctgaggtcag ctacggtgag
    11161 catctcaagg gtcagttacc ttggaggtgc tgggcagcac ccgaccaggt gacgtagagg
    11221 taaacgagcg gcgccaccac gagcagcgcg tcgattcggt cgaggatccc gccgtgaccc
    11281 ggcacgaccg cgccggaatc tttgatgcca gtcgagcgct tcaggagcga ttcgacgaga
    11341 tcgccgaatt gtccaagagc acccgcaacg acggcgagcg gtagcacctc tgccaagttg
    11401 atttccggca ggaaccaaaa gcgcgcagcg agcgctccgc atgccgctcc gaccaacgcc
    11461 ccgacgaagc ccgcgcgcgt cttcttcggg ctcaccgctt cgtaaagctt ggtttttccg
    11521 agaaagcgcc cgaagaagta accgcccgta tcggcgagcc acgcgaacgt cagtgcaagt
    11581 acgacccacg cagggccaga gggaccgagc tcggacttca agagcgcgag cgtggtgagt
    11641 gacgcgccga catagagcgg ccccgcgacg cccgccatga tgcgaatccc cgcggtcgcg
    11701 atggtcccga gccgaacgag cggcaccatg atgcccaagg tcgggaccaa gagcaggatc
    11761 gtgaggagca ctcgggcgtc gttcggtcgc cagtaaagag ccagcgagac cgcagccgtg
    11821 aaagctacgc cgatgccttg tgagatcgca tcgcccgggt gcgtcatgcg aaacaattcg
    11881 aacgcaccga gcacggccac gagcaagacg agcgcgtaga agccccacgc cggtccgcga
    11941 aacaagagca gcaacagggg tggaacgacg acggccgccg tcagcaaacg cagggcgagg
    12001 ttactgcgaa ccacgcgcat ccgtaacagc ggcggccccc ctgctcaagc cctccgcgcg
    12061 gacgtacgcg ggtggctagc cattctgcgg aatttttcag ttggctggcg ggcatccgcg
    12121 agtgctcttg cgcgtcacga actccgccgc taggaccggg ccaggtgctt ggttctccgg
    12181 tcggctatct gcgacggcgt ggcgtcgctc tcgccttggc aggctccgct gccctcgcgg
    12241 cgcgctgcgc gagcgacggt ccgcccgcgc ggacgctcga tggcctcgcg cagcttctcg
    12301 gcaacgccgc gcatggcgtc gtcgagccgg aagcgattgt ctgggagccg agcgcggggt
    12361 tgctcgtcga taccttcgtc gggcgccgcg tgctcttcct cgcatcggcg aaggccgggg
    12421 agccgcgcga cgtctatcgc gctcgggtcc ggctcgcgct cgacggcgct ccgattcacg
    12481 tctccgacat caagaacctc accgacacgc cccatggcga cgacgtggcg ctcgaaggcg
    12541 atggagagcg cgtctcgttc gcgacgttgg cgttcggacg cattcaagcc atcagcgtgc
    12601 tcgagaccac cggggtgcga gacgccgacc gaccgagcgg cgctctgaat cgggcgttgt
    12661 ttgcgctgag ctcgttcgac aagaccggga gcttggcggg cgtcgggcgc accgacatcg
    12721 tgctcgatgt gcccgctcgc tcggcgcgcc tcacgatgga cgccgcgcac ttggtcgtcg
    12781 acttcggtga gcgcgggcgc gggctcagtt acgatctcga gcggcgcacc gtgcgcggta
    12841 ccgatggcgg ggaagcctac gccgcgcgcg tcgttcccga agcctacggc gcgaaacctc
    12901 cgctgctctg ggctgtcgat acggtgcgcg gcgtcgtggg accgcggccc atcgcgtggc
    12961 tcgagaacgt cgtctttggc gccaaag
//
LOCUS       DASTKS010000514         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1196795, whole genome shotgun sequence.
ACCESSION   DASTKS010000514 DASTKS010000000
VERSION     DASTKS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196795"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFQ35_10675"
     CDS             <1..700
                     /locus_tag="VFQ35_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9931144.1"
                     /translation="LVPAPNAPRILVVEDEPSIRQGLLDVLVFHGYGADFAADGATAL
                     RRIADSEFDLVVLDVMLPVLDGFSVCEQLRRTQPELPVLMLTAKNSEDDVLRGFEAGA
                     DDYVTKPFSIRQLLARVHALLKRTRKGKTDAFSVGPLEVDPERAALHAPDGDQELTQR
                     EIEMLRLLAEEPGRIVSRRVLLREVWGMQNVDQVETRTVDVHVGKLRKKLGRHGELLE
                     TVRGQGYRLCTRTR"
     gene            682..1209
                     /locus_tag="VFQ35_10680"
     CDS             682..1209
                     /locus_tag="VFQ35_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931145.1"
                     /translation="MHPNPLTKLRLSFLALGALLFVPLLWLIHSVNARVEAQRRLRHQ
                     VVAERIFDELERELTRVLREESSRPSSAFDRAGPDSAPPYVVGYFRTENEAPTALRVQ
                     GPDGKARVDRALVAANLVTTLPSSLQGAKEPPPKSQRIPAPATPSPASRSMSPAEVLR
                     KLNRASEDRQEAPTR"
     gene            1215..2372
                     /locus_tag="VFQ35_10685"
     CDS             1215..2372
                     /locus_tag="VFQ35_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931146.1"
                     /translation="MTRRWLAVAVAVAALLSCVSAEASEDFRVVPAEPGTLVLVRPRA
                     NASDKEGVVIDSETLVQILKARVLQARGLDEVAELSSLGSYGRREASLVGGYRFAHEF
                     GPPFESVSASLNLRPLADPEDGSLVVPLSIVLCFSLALGLIALYRMAAAQVRFAERRN
                     NFVSAVSHELKTPLTAIRMHAEMLEEDLVDGDAKRHEYYRTITKESERLTRLIDNVLS
                     LAQLERGTRRLSLVLGDVRPAVVEALEMLRPQVESQGFVLEFEAAPSLPSVRYDPDAL
                     KQVLFNLVENALKYGRDASERRITVRLEPRGNGVELSVRDRGPGVLREHMKSIFEPFF
                     RAEHELTRKNQGTGIGLSLVQGLARAMEASVEGENAHPGFRVRLELRAEQG"
BASE COUNT          461 a          872 c          826 g          446 t
ORIGIN      
        1 gctcgtgcct gccccgaacg ccccgcgaat cctggtagtg gaggacgagc cgagtatccg
       61 ccagggccta ctcgatgtgc tcgtctttca cggctacggc gccgacttcg ccgccgacgg
      121 cgcaaccgcg cttcggcgta tcgctgactc ggagttcgac ttggtcgtgc tcgacgtgat
      181 gctgccagtg ctcgatggct tcagcgtgtg cgagcagctg cgacgcaccc agcccgaatt
      241 gccggtcttg atgctcaccg cgaagaactc ggaggacgac gtcctccgcg gcttcgaggc
      301 cggcgccgac gactacgtaa ccaagccgtt tagtattcgg cagctcctcg cgcgagtgca
      361 cgcgctgctc aagcgcacgc ggaaggggaa gacggatgct ttctcggtcg ggccattgga
      421 ggtagacccc gagcgcgccg cgctacacgc gccggacggc gatcaggagc tcacgcagcg
      481 agaaatcgaa atgctgaggc tgctcgcgga ggagccgggc cgcatcgtga gtcgtcgcgt
      541 gctgctgcga gaggtgtggg gcatgcagaa cgtagatcag gtcgaaacgc gcacggtcga
      601 cgttcacgtc ggcaagcttc gaaaaaagct cggtcgtcac ggggagctgc tcgaaaccgt
      661 ccgaggacaa ggataccgtc tgtgcacccg aacccgctaa ccaagctacg gctctcgttc
      721 ctcgcccttg gagcgttgtt gttcgttccg ctgctgtggc tcattcactc ggtgaatgca
      781 cgcgtcgaag ctcagcggcg cctgcggcat caggtggtcg ccgagcgcat cttcgacgag
      841 ctcgagcgcg agctcacgcg ggtcttgaga gaagagtcgt cgcgcccgag ctccgccttc
      901 gaccgagcgg gcccggactc cgcgccgccg tacgtcgtcg gttacttccg aaccgaaaac
      961 gaggcgccca ccgccttgcg tgttcagggg ccggacggaa aggctcgcgt agaccgcgcg
     1021 ctcgtggccg cgaacctcgt caccaccctc ccgtcgagtc tgcaaggcgc gaaagagccg
     1081 ccgccgaaga gccagagaat ccctgctccg gcaaccccct cccccgcctc gcgctcgatg
     1141 agccccgcag aggtgctgcg caagctgaac cgcgcgagcg aagaccgaca agaggccccc
     1201 acgcgatgac gaccgtgact cgacgttggc tcgcggtcgc ggtcgcggtc gcggccctgc
     1261 tttcgtgcgt ttcggcggag gcgagcgagg acttccgcgt cgtccccgcc gagcccggaa
     1321 cgctcgtctt ggtacgtccc cgcgcaaacg cctccgacaa agaaggtgtc gtcatcgact
     1381 cggagacgct cgtccagatc ctcaaggcgc gcgtcctcca agcccgcggg ctcgacgaag
     1441 tcgcggagct gagctccctc ggctcgtacg gccgtcgcga ggcttcgctc gtcggtggct
     1501 atcggttcgc ccacgagttc gggcctccgt tcgagtccgt gagcgcgagc ttgaacttac
     1561 gcccgctcgc ggatcccgaa gacggttcgc tggtcgttcc tctcagcatc gtgctctgct
     1621 tctcgctggc gctcgggctg attgctctct atcgaatggc ggcagcccag gtacgcttcg
     1681 cggagcgccg caacaacttc gtgtcggccg tgtcgcacga gctcaagacg ccgctcacgg
     1741 cgatccgcat gcatgccgag atgctagaag aagatctcgt cgacggcgac gccaagcgcc
     1801 acgaatacta ccgaaccatc accaaagaga gcgagcgtct gactcggctc atcgacaacg
     1861 tgctcagcct ggcccaacta gagcgcggga cccgaaggtt gtcgctcgtg ctgggcgacg
     1921 tacggccagc cgtcgtcgaa gcgctcgaaa tgttgcgccc ccaagtcgag tctcagggct
     1981 tcgtgctcga gttcgaagcc gcaccgagcc tgccaagcgt tcgctatgat cccgacgccc
     2041 tgaaacaggt gttgttcaac ctcgtcgaaa acgcgctgaa gtatggccgg gacgcgagcg
     2101 aacggcgcat caccgtgcgc ttggaaccgc gcggaaacgg cgtcgagctc agcgtgcgcg
     2161 accggggccc cggcgtgctc cgcgaacaca tgaagtcgat cttcgagccg ttcttccgcg
     2221 ccgagcacga gctaacgcga aaaaaccagg gaaccggcat cgggttatcg ctcgtccagg
     2281 ggctcgcgcg agccatggaa gcaagcgtcg agggtgaaaa tgcgcaccct ggcttccgcg
     2341 tccgcctcga actgcgcgcg gagcaaggct aggaattccg gacaccttct cgtatttaat
     2401 aaatgaagca tcgatttccg ccggcttcag cgacgcgcaa ttttgccgtc tgagccggtt
     2461 tgcaagcgtc agggacactc tagccaagag tcgcccgcgt ctctcgacat ggttaccccg
     2521 ctgaaattcg ttggcgacga gcgcgcgctc cttcaggcgc tccgggcccg gcatccgggt
     2581 gcggtggccg ccttctacga cgccc
//
LOCUS       DASTKS010000515         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_973532, whole genome shotgun sequence.
ACCESSION   DASTKS010000515 DASTKS010000000
VERSION     DASTKS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973532"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFQ35_10690"
     CDS             complement(<1..830)
                     /locus_tag="VFQ35_10690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931147.1"
                     /translation="MANEPHTVGTIVDRRYRLKREIARGAAGAVYEAVHLYTRRAVAV
                     KLLNAAHINVEETRIRLLREAAAMATVRHPGVAQVLDAGELEQLGPYVVMELLEGRTL
                     QGILAVRQRIGVHETVQIGRQIGEALAAAHAHGVVHRDIKPSNLFVARDETGREVCKI
                     IDFGIARDQADQRKLTLHGAVLGTPEYMAPEQMLGQSEIDGRADIYSVGATLYECLSG
                     AVPFEGNYAEVLLKSATQPLAPIRAKNPTVPAELVSVVEKALARDVADRYPSMVAFLE
                     A"
     gene            complement(921..2297)
                     /locus_tag="VFQ35_10695"
     CDS             complement(921..2297)
                     /locus_tag="VFQ35_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931148.1"
                     /translation="MFRSRFSVLALTLSTLAACGGASGSARIGEDPVSRKTPPSVRAR
                     APEHSEADSDKEDEDAPEAAPATPAASAQEPPTPTYDATQVRAAVDATAAAPDERTRL
                     GVRLAVVEQGPDAPWLIAVVNRGTEPVRVVHDLRTLNLEVTPPAPEPDPKKKAKPRAP
                     KPVTCALPRGIVPSEEEKTLELTLEPGEGLIDSFDPRLYCLPTAGKSVLTEGASVVAR
                     LGWQEKTRAVWTKGKKVTRVLEQSAPFVARRVRVAGSLPEAEEPKTKVEKDDPASEPK
                     SDREAVKQLVAAPISLGEAYAPQKPPPSDSLDLVLTRGSDASTERDATISVSLVNHSK
                     KAERVLFRRESLTFEVSGPDGLVACEPGPDNRAPDRSSIALLKPGRRLSSTSRLIELC
                     PQGAMRRPGLYLVHARFDGLKGGDSERPPTFAGRVVSREPVAIRVRRGWGEMPAQRQP
                     ERVRVGTP"
     gene            2381..3016
                     /gene="thiE"
                     /locus_tag="VFQ35_10700"
     CDS             2381..3016
                     /gene="thiE"
                     /locus_tag="VFQ35_10700"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET9931149.1"
                     /translation="MSRLYPIVDLDAISRANLEPLAFSERVVYAQPPVLQLRAKRTPA
                     RVTLDIARGLSALCKRFDVRFFVNDRPDLAVLAGADGVHVGQEDLSVSEVRAVAPSLQ
                     VGVSTHNIEQLTRALAERPSYVAFGPVFPTGSKENPDPVVGLGALERARELADAAGIR
                     LVAIGGIDLERARLLSKLDVDAAVISALVAPSLAEVERRAAELDHVLAFGQ"
     gene            complement(3019..3987)
                     /locus_tag="VFQ35_10705"
     CDS             complement(3019..3987)
                     /locus_tag="VFQ35_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HET9931150.1"
                     /translation="MSFDEIWGQDPAIQTLKRALTTGRVHHAYRFEGPPGVGKEMAAW
                     RFARALVCERGGLGCGECSACRRAMTLSENEPHVPIHPDVVLLARGLYRSVTGQAEAA
                     GIGVEQVRRIVLERVGYSPHEGRALVFIVRDADELTQQAANSLLKTLEEPPARTHFVL
                     VTSRPNRLLDTIRSRAQPLRFSPLPEAVIARILEKNGRDPKLAPFAQGSAELALALAD
                     PEALSEREAFVSALQQALDAKDLAMAIKFAEAQKGDRDSLKAELAFFAQKLAASARES
                     VQNDPAAAARSARRYAAVVAAMTDIESNVQAALVLETLIQTLRRAS"
     gene            4039..5130
                     /gene="hisC"
                     /locus_tag="VFQ35_10710"
     CDS             4039..5130
                     /gene="hisC"
                     /locus_tag="VFQ35_10710"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9931151.1"
                     /translation="MPTHPVADLLRPELAELSAYVPHPGQYAIRLDANEAPALLPPSV
                     RARLAEAMGALAYERYPDASVEKLRAALAQHSGVGADEVLVGVGSDELIAILLSCLSR
                     PRPGLDAPVVLTTTPSFVMYSMSARLRGQRVLEVPLSADWDLSVQSMLAATRVAEPSV
                     VFIASPNNPTGNKMSLDRLVAVIEAMPRSLVIVDEAYVDYSDVNQLELYRRYPNVGVL
                     RTLSKVGFAALRVGWLMAAPELIRELDKFRPPYNMSGLSQELATVVVSELWPEIRTMI
                     ASVVSERARVTEALNGLPGLSVTPSQANFVWLKTEKPAQVVFDGLRSRGILVRSFHTR
                     GGRLANQLRVTIGSAEENDALLQALKDLG"
BASE COUNT          803 a         1808 c         1781 g          906 t
ORIGIN      
        1 gcctcgagga acgcgaccat cgaggggtag cgatcggcga cgtcgcgggc cagcgccttc
       61 tcgaccacgc tcacgagttc ggcgggcacg gtagggttct ttgcccggat tggggcgagc
      121 ggctgcgtcg ccgacttcaa cagcacctcc gcgtagttac cttcgaaggg caccgcgccg
      181 ctcaaacact cgtagagagt cgcgccgacc gagtagatgt cggcgcggcc gtcgatctcg
      241 gattggccga gcatttgttc tggcgccatg tactccggag tgccgagtac ggctccgtgg
      301 agcgtgagct tgcgctggtc ggcttgatcg cgcgcaatgc cgaagtcgat gattttgcag
      361 acctcgcggc ccgtctcgtc ccgcgccacg aagaggttgc tcggcttgat gtcgcggtga
      421 acgaccccgt gcgcgtgggc ggccgcgagc gcttcgccga tttgtcggcc aatctgtacg
      481 gtctcgtgca cgccgatgcg ctgccgcacg gcgaggatcc cttggagcgt acgtccctcg
      541 agcagttcca tcacgacgta ggggccgagt tgctcgagct cccctgcgtc gagcacctgg
      601 gccacccccg gatgccgcac cgtcgccatc gccgcggcct cgcgcaacag ccggatgcgc
      661 gtctcttcga cgttgatgtg cgcggcgttc aggagcttca ccgcgaccgc gcgccgcgtg
      721 tagagatgca ccgcttcgta gacggcgccc gcggcgccgc gtgcgatttc gcgcttcagc
      781 cggtagcgcc gatcgacgat cgtcccgacg gtgtgcggct cgttcgccat gcgcggctca
      841 gcccgaggcc cctctcggca aaacggctct aacctcctcg cgaacgcgca tcgcgtctca
      901 aggctatcac gggatcgtcg ctagggagtc ccgacgcgca cgcgctcggg ttggcgctgc
      961 gcaggcattt cgccccagcc gcgccgcacc cgaatcgcga ccggctcccg gctaactacg
     1021 cgcccggcga acgtgggagg acgctcggaa tcgccgcctt ttaggccgtc gaagcgcgcg
     1081 tgcaccaggt agaggcccgg gcgacgcatc gcaccttggg ggcagagctc gatcagacga
     1141 ctcgtcgagc tgagccgccg gcccggcttg agcagggcga tgctggagcg gtcaggtgcg
     1201 cgattgtcgg gtccgggctc acacgcgacg agcccgtccg ggcctgatac ctcgaaggtg
     1261 agagactcgc gacgaaagag cacgcgctct gccttcttcg agtggttcac gagcgatacc
     1321 gagatggtcg cgtcgcgctc ggtcgacgcg tccgagccgc gcgtgagcac gaggtcgagc
     1381 gaatcgctgg gcgggggctt ctgcggagcg taggcctcgc cgagcgagat tggcgcagcc
     1441 acgagttgct tcaccgcctc gcggtcgctc ttgggttcac tcgcgggatc gtccttttcg
     1501 accttggtct tcggctcttc cgcttcggga aggctccctg caaccctcac tcggcgagca
     1561 acgaacggcg ccgactgctc gagtactcgc gtgaccttct tgcccttcgt ccagaccgct
     1621 cgggtcttct cttgccaacc caggcgcgcc acgacgctcg ccccttcggt gagcaccgac
     1681 ttgcccgcgg tgggcaagca atagagacga ggatcgaagc tatcgatgag gccctcgccc
     1741 ggctcgagcg taagctccaa ggtcttctct tcttcgctcg gaacgatgcc gcgcggcagg
     1801 gcgcacgtga ccggcttcgg tgcgcgcggc ttcgccttct tcttcggatc gggctcgggc
     1861 gcgggcggcg tcacctcgag gtttagggtg cgcagatcgt ggacgacgcg aacgggctcc
     1921 gtgccgcgat tgacgacggc gatgagccaa ggcgcgtccg gcccctgctc caccaccgcg
     1981 agccgcactc caaggcgcgt ccgctcgtcc ggcgcggccg cggtcgcatc cacggccgca
     2041 cggacttgag tggcgtcgta cgtcggcgtc ggcggctcct gcgcgctagc ggcgggggtc
     2101 gccggtgcgg cctcgggcgc atcctcgtcc tccttgtccg agtcggcttc ggagtgttct
     2161 ggggcgcgcg cgcgaacgct cggcggggtc ttcctgctga ccggatcttc cccgatgcga
     2221 gcactgcccg atgcgccgcc gcatgccgcg agggtcgaga gcgtgagggc gagcaccgag
     2281 aagcgagaac ggaacatggt ttccttttag tgccgggccg cacgcggaac aaaccacaaa
     2341 tggcgggtgc gaacgcgcct gccaagctac gctccgccga gtgagtcgcc tgtatcccat
     2401 cgtcgacctc gacgcgattt cccgcgcaaa cctggagccg ttggcgttct cggagcgggt
     2461 cgtgtacgcg cagccgccgg tcctgcagct ccgagcgaag cgaacgcctg cccgcgtgac
     2521 cctcgacatc gcgaggggtt tgagcgctct ctgtaagcgc ttcgacgtgc gctttttcgt
     2581 caacgaccgc cccgatctcg cggtgctcgc cggcgcggac ggcgtccacg tcggccagga
     2641 ggacctcagc gtcagcgagg tgcgagcggt ggcgccgtca ctccaggtcg gggtttcgac
     2701 gcacaacatc gagcaactca cgcgggctct cgcggagcgg ccgagctacg tggcgttcgg
     2761 ccccgtcttc cccaccgggt cgaaagagaa tccggatccg gtcgtcgggc tcggcgcgct
     2821 cgaacgagcg agagagctcg ccgacgcggc ggggatccgc ttggtcgcga ttggcggcat
     2881 cgacctcgaa cgcgcacgct tgctcagcaa gctcgacgta gacgccgccg tgatctccgc
     2941 gctcgtcgct ccgtcgctcg ccgaggtcga gcggcgcgcc gcggagctcg accacgtgct
     3001 cgcctttggg cagtgacgtc aggacgcgcg gcgcagcgtc tgaatcagcg tttcgagaac
     3061 gagcgctgcc tggacgttgc tctcgatgtc cgtcatcgcc gcaaccaccg ccgcgtagcg
     3121 tcgcgctgag cgcgcggcgg cggcggggtc gttttgaacg ctttcgcggg cgctcgcggc
     3181 gagcttttgc gcaaagaacg cgagctccgc cttgaggcta tctcgatcgc ccttttgagc
     3241 ttcggcaaac ttgatagcca tcgcgaggtc cttcgcgtcg agagcctgct gcaaagcaga
     3301 gacgaacgcc tcgcgctccg acaacgcctc tggatccgcg agcgctagcg cgagctcagc
     3361 gcttccctga gcgaacgggg caagcttcgg gtctcgtcca tttttctcca aaatccgagc
     3421 gatgaccgct tcgggcagcg gactgaagcg cagcggctgg gcgcgcgagc gaatcgtgtc
     3481 gagcaatcgg ttcggacggc tcgtgacgag cacgaagtga gtgcgagctg gaggctcttc
     3541 gagcgttttc agcagcgagt tcgccgcctg ctgcgtgagc tcgtccgcgt cgcgtacgat
     3601 gaagaccagc gcgcgccctt cgtgcgggga gtaccctacc cgttcgagca cgattcggcg
     3661 aacctgctcg acgccgatgc cggccgcctc cgcctgacca gtgacgctgc ggtagaggcc
     3721 gcgcgccagg aggacgacgt ccgggtggat ggggacgtgc ggctcgttct cggacagggt
     3781 catggcgcga cgacaggccg aacactcgcc acacccgagc ccgccccgtt cgcagaccaa
     3841 agcccgagcg aaccgccacg ctgccatctc cttgccgacg ccgggcggcc cctcgaaccg
     3901 ataggcgtgg tggacgcgcc cggtcgtcag cgcgcgcttc aaggtctgga tcgcggggtc
     3961 ctggccccaa atctcatcga aactcatggc ggacgggctt ctcgggctga gagggcgtga
     4021 tactactccg gccgccgcat gcccacgcac ccggttgccg accttttgcg tcccgagctc
     4081 gcggagctca gcgcctacgt accccatccc ggccagtacg ccatccgcct ggacgcgaat
     4141 gaagcgccgg cgctgcttcc cccatcggtt cgggcccggt tggcggaggc gatgggcgcg
     4201 ctcgcctacg agcgttaccc cgacgcgagc gtcgaaaagc ttcgcgccgc cctcgcgcag
     4261 cacagcgggg tcggcgccga cgaagtactg gttggcgtcg gcagtgacga gctcatcgcg
     4321 atcctgctga gctgcctgtc gcgacctcga cccggcctcg acgcgcccgt cgtgctcacc
     4381 acgaccccga gcttcgtgat gtactcgatg agcgcgcggc tccgcgggca gcgcgtcttg
     4441 gaggtgccgc tcagcgccga ttgggacttg tcggtgcaaa gcatgctcgc cgcgacacgc
     4501 gtcgccgagc cgagcgtcgt cttcatcgcg agcccgaaca acccaaccgg aaacaagatg
     4561 tcgctcgatc ggctcgtcgc cgtgatcgag gcgatgccgc gctcgctcgt catcgtcgac
     4621 gaagcgtacg tcgactattc ggacgtgaac cagctcgagc tctaccgccg ctacccgaac
     4681 gtcggtgtgc ttcgcaccct gtccaaggtg ggctttgccg cgctccgcgt cggctggctg
     4741 atggccgctc cagagctcat ccgcgagctc gacaagttcc gaccgccgta caacatgtcg
     4801 ggcctttcac aggagctcgc gacggtagta gtgagcgagc tctggcccga aatccgcacc
     4861 atgatcgcaa gcgtcgtgag cgaacgggcg cgcgtgaccg aagcgttgaa cggcctgccc
     4921 gggctctcgg tgactccgag ccaggcgaac ttcgtttggc tcaagaccga aaagccggcg
     4981 caagtcgtgt tcgatggcct tcggagccgc ggaattctgg tgcggagctt tcatacgcgc
     5041 ggcgggcgct tggcgaacca gctgcgtgtg acgatcgggt cggccgaaga gaacgatgct
     5101 ctcctgcaag cgttgaagga tttaggctga cgcacgatgc gccggcgctt cgctccaccg
     5161 tttctagtcg tcgcgctcct gaccacgagc ggctgcgcct tcacggccgg gagcgtcacg
     5221 cgcatcgccg acggtgtcga gcacgagcag gcggcggtct cgacggaggc ctacgcatac
     5281 tacgcgcgcg gcgtcgtg
//
LOCUS       DASTKS010000516         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_81808,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000516 DASTKS010000000
VERSION     DASTKS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81808"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFQ35_10715"
     CDS             complement(<1..297)
                     /locus_tag="VFQ35_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931152.1"
                     /translation="MTRDRAERRALRHLRAREAELRREALSVGGSANAQLGPDPLRLC
                     ALGERLVGLLHGTSELALLDASLNELERTKGPERPVACATFGRNELAVIGSTPEL"
     gene            543..1052
                     /locus_tag="VFQ35_10720"
     CDS             543..1052
                     /locus_tag="VFQ35_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931153.1"
                     /translation="MSTPSLSVQEQAERWGAEAKRLSDAGRDLPAFDLYRKAADALPG
                     APWLQHRTAEVARKLKRDELAIEYFRRSAAAFKKAGFDKRAVAPLRMAWTIARGDLKQ
                     FGEQFVESAHDLSHLLGALGLGADAASTLEETEESLRRMGLAHLLNPSMPPSRNAPKS
                     IYPTMRPPH"
     gene            1167..3707
                     /locus_tag="VFQ35_10725"
     CDS             1167..3707
                     /locus_tag="VFQ35_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9931154.1"
                     /translation="MSSKPEAIPSVLARLAALQARAPWHFVVVALLTLLPAAWSTSRL
                     GFRPDFAELLPDNKDSVIVMRKNAKRLAGASTLSLVIQIDDGSRRQSLRTFVDTLVPK
                     LRALGPDWIGSVDYGVKETQQFFEDNKLLYAKLDDLQRAHDRIVERYDWEVAKAQGTL
                     LEEDEAPPPISAEVIEKELKGKSAEPTKSKDLPAYPGGYYESADGTYSAVLIRTPVSG
                     RAKVNELLDRVNKLVAEVNPKSFDPSMHVGFTGDLITSQEEYDAIVTDLSEVGAGGVI
                     GVLASVFLFFLRVRTVMTMGATLLIGLLWTFGITHYTIGFLNSSTGFLVSIIAGNGIN
                     YGIMYMARYVEARRDQGLPPTEAVLVAHRDSWLPTLASAGTAALAYGSLMVTNFRGFK
                     HFGVIGAYGMMLCWLTTYLFMPAILVASERVLPAYKPLPPGHAPRRQVRYSAVFAKLA
                     FGFPRAVSIVGVVIGLASIALTVRYFERDPMEYNMRNVRTDSAASESGARALSDKVDK
                     VVGRLGQEGMAVMTDRVDQVPLLVAELEKRERAAPPDAKPFEKVVSVFSLLPSDQEKK
                     LPLVTEMVDRIRRAERRNLIPAADWAKIEPHLPKGELKPIGIAELPDQVARPFTERDG
                     SRGRIVYIAPKSGQSVWNAKYLMRWADSFRSVTLPTGEVIKGSGRAVIFADMIEAIGE
                     DAPKAIAMSALGSILVILLAFRGDKLAFGVFFPWLIGITTLIAFMLLKDIRLNFLNFV
                     AIPITIGIGAEYAHNMVQRYRAEGDERLEHVIVSTGSAVTLCSLTTTIGYLVLLMSIN
                     RGIRSFGLSAAVGEITCLCAAVLWLPSLLVWRARRKAAKAGSPASETR"
     gene            3720..>4587
                     /gene="nagZ"
                     /locus_tag="VFQ35_10730"
     CDS             3720..>4587
                     /gene="nagZ"
                     /locus_tag="VFQ35_10730"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET9931155.1"
                     /translation="MPKDLVARAARLVCVGFSGTEVSSELRELVRLGVRSVILFARNV
                     REPEQVLSLTQEIKALTTEPIAVAVDQEGGPVRRLRAGFTDVPAMRCVGAANDPLLAE
                     RTGMLLGRELRAVGIDWNFAPVLDVDTNPANPVIGVRAFDRSPARVAELGVALARGLG
                     LAGVAACAKHFPGHGDTEQDSHLALPRLTHSLDRLLGVELVPFQAAVRAAIPSVMSAH
                     VLFSALDPNYPATLSPLVLKGLLRERLGYTGLVVSDDLEMNAIVEHYGIETAIFRGLL
                     AGVDCFLVCHTPE"
BASE COUNT          810 a         1511 c         1451 g          815 t
ORIGIN      
        1 gagctccgga gtcgagccga tcaccgcgag ctcgttgcgg ccgaacgtcg cacacgcgac
       61 gggccgctcg ggccccttcg ttcgctcgag ctcgttcaac gaagcgtcga gtagcgcgag
      121 ctccgaagtt ccgtgaagga gcccgacgag gcgctctccc aaggcgcaca ggcgcagcgg
      181 gtcgggcccc aactgtgcat tcgcgctacc acctaccgaa agcgcttcac gccgtagctc
      241 cgcttcgcgc gcccgcaaat gccggagtgc gcgtcgctcg gccctatcgc gtgtcaccga
      301 gcgtgtcgtt accgtcttcg cttccttctc cggcggcgac ggcctcgagc aactcgataa
      361 cgagacgatg aggggcaaga tcgcagcgag gtgcgtcgcg cttctctctt tgagccgacg
      421 cccgcggcgc gcgtcccgaa gagagacatc gagcgcacca cctgccactc gcgcttcacc
      481 aatggttccg attagatggc tcgctgtcgg gatacggcac gtcgcgtgct gaatgcgtca
      541 tcgtgagcac accgagcctg tccgttcaag agcaagcaga acgctgggga gccgaagcaa
      601 agcgcctatc cgacgctggg cgggatctcc ctgcgttcga tctgtaccgc aaagccgcgg
      661 atgccctgcc gggagcgccc tggctacagc accgcaccgc cgaagtcgcc cggaagttga
      721 agcgagacga gctcgccatc gagtacttcc gtcgctcggc tgcagccttc aaaaaggccg
      781 gcttcgacaa gcgcgcggtc gcgccgctgc gcatggcgtg gaccatcgcg cgcggtgact
      841 tgaagcagtt cggcgagcag ttcgtggaga gcgcgcacga cctctctcac ttgctcggtg
      901 cgctcggcct tggcgcggac gctgcgagca cgctcgaaga gacggaagag tcgttgaggc
      961 gcatgggcct cgcacatctc ttgaacccgt cgatgcctcc gtcgcgcaac gccccgaaga
     1021 gcatctatcc gacgatgcgg cctccgcact aggggagcag ctgcgcggcg aacccagcgc
     1081 cgcgcgatga cacggccacg caccgaaccg gtggccggcg ctagcaagcg gtcgtctggc
     1141 gcgaatcgtg taatctgcgc ggctcgatgt catccaagcc cgaggcgatc ccctccgtgc
     1201 tggcgcgcct ggccgcgttg caagcgcgag ctccctggca cttcgtcgtg gtggcgctcc
     1261 tcacgttgct gcccgcagcc tggtccacga gccgcctcgg ctttaggccg gatttcgcgg
     1321 agctcctccc ggacaacaag gacagcgtca tcgtcatgcg caagaacgcc aagcgacttg
     1381 cgggcgcctc gacgctgagc ctggtgattc aaatcgacga cggttctcgg cgacagtcac
     1441 tgagaacttt cgtcgacacg ctggtgccga agcttcgcgc gctggggccg gattggatcg
     1501 ggtcggtcga ctacggggtg aaagagacgc agcagttctt cgaggacaac aagctcctct
     1561 acgcgaagct cgacgatttg cagcgcgcgc acgaccgcat cgtcgaacga tacgattggg
     1621 aggtcgcgaa agcccaaggc acgctgctcg aagaggacga agcgcccccg cccatcagcg
     1681 ccgaggtcat cgaaaaagag ctgaagggaa agagcgccga gcccacgaag agcaaggatc
     1741 tacccgcgta cccaggcggc tactacgagt ctgccgacgg gacctacagc gcggtcttga
     1801 tccgaacgcc ggtctccggg cgcgcgaagg tgaacgagct gctcgatcgc gtgaacaagc
     1861 tcgtggccga ggtgaacccg aagtcgttcg acccgagcat gcacgttggg ttcaccggag
     1921 acctcatcac gagccaggaa gagtacgacg ctatcgtcac cgatttgagt gaagtgggcg
     1981 cgggcggcgt catcggcgta ctcgccagcg tctttctgtt tttcttgaga gtgcgcacgg
     2041 tgatgacgat gggcgcgacg ctgctcatcg gactcttgtg gaccttcggc atcacgcact
     2101 acacgatcgg atttttgaat agctcgaccg gatttttggt cagcatcatc gccggcaacg
     2161 gcatcaacta cggcatcatg tacatggcgc gctacgtgga agcgcgccga gatcaggggc
     2221 tccctccgac cgaggccgtg ctcgtcgcgc accgcgattc gtggctaccg acgctcgctt
     2281 ccgccgggac cgcggcgctc gcgtacggct cgttgatggt cacgaacttc cgcggcttca
     2341 agcactttgg agtgattggg gcgtacggaa tgatgctgtg ctggctcacg acgtatctgt
     2401 tcatgccggc gatcctggta gcgagcgagc gcgtcttgcc ggcgtacaaa ccgctgccgc
     2461 ccggtcacgc cccgcgccga caagtgcgct acagcgccgt cttcgcgaag ctcgcgttcg
     2521 gttttccgcg cgccgtgagc atcgtcggtg tcgtgatcgg gctcgcgtcg atcgcgctca
     2581 ccgtccgcta cttcgagcgc gatccgatgg aatacaacat gcgcaacgtg cgcaccgaca
     2641 gcgccgccag cgagagcggc gcgcgggccc tgtccgacaa agtcgacaag gtcgtcgggc
     2701 gactcggcca agagggcatg gccgtgatga cggatcgcgt cgaccaggtt ccgttgctcg
     2761 tcgcagagct cgagaagcgc gagcgagccg ccccgcccga cgcaaagccg ttcgagaagg
     2821 tcgtgagcgt tttctccctc ttgccgagcg accaagagaa gaagctcccg ctcgtgaccg
     2881 agatggtcga ccgcatccgc cgcgccgagc gccgtaacct cattccagcc gcggattggg
     2941 ccaaaatcga gccccacctg cccaaagggg agctcaagcc aatcggcatc gccgagctcc
     3001 ccgaccaagt cgcgcggccc ttcaccgaac gcgacggctc gcgcggtcgc atcgtgtaca
     3061 tcgctcccaa gtcgggtcag agcgtttgga acgcaaagta cctcatgcgc tgggcggata
     3121 gctttcgctc cgtgacgttg ccgaccggcg aagtgatcaa aggctctggt cgcgccgtga
     3181 tcttcgcgga catgatcgaa gcgatcgggg aggacgcgcc gaaggcaatc gcgatgtcgg
     3241 cgctcggctc gatcctcgtc attctgctcg cgttccgcgg cgacaagctc gcgttcggcg
     3301 tcttctttcc ctggctcatc ggaatcacga cccttatcgc gttcatgttg ctgaaggaca
     3361 ttcgcctcaa ctttttgaac ttcgtcgcga tcccgatcac gatcggcatc ggcgccgagt
     3421 acgcgcacaa catggttcag cgctaccgcg ccgagggcga cgaacggctc gagcacgtga
     3481 tcgtcagcac gggcagcgcc gtgacgttgt gctcattgac cacgaccatc gggtacttgg
     3541 tgctgctgat gtccatcaac cggggcatcc gaagcttcgg gctgagcgct gcggtcggcg
     3601 aaatcacctg cctctgcgcg gccgtgcttt ggctcccatc gctcttggtg tggcgagccc
     3661 gccgaaaggc ggcaaaggct ggctctccgg cgagcgaaac gcgctaatct gccctcccga
     3721 tgcccaagga tctcgtcgcg cgcgctgcgc gcctagtgtg cgtcggattc tccggaacgg
     3781 aggtttcgag cgagctcaga gagctcgttc ggctcggggt acgcagcgtc atcttgttcg
     3841 ctcgcaacgt gcgtgagccg gagcaggtgc tctcgctgac ccaagagatc aaagcgctca
     3901 ccacagaacc catcgcggtc gcggtcgacc aagagggagg gcccgtgcgc cgccttcgcg
     3961 ctggcttcac ggacgtgcct gcgatgcgat gcgtcggtgc cgcgaatgat ccgttgctcg
     4021 cggagcgcac agggatgctg ctaggtcgtg aactacgcgc cgtcggcatc gactggaatt
     4081 tcgcccccgt gttggacgtg gacacgaacc cagccaatcc agtcatcggc gtacgcgctt
     4141 tcgatcgctc gccggctcgg gtggcagagc tcggggtggc actcgcccgc ggccttgggc
     4201 tcgcaggggt agcggcctgc gccaagcact ttccgggaca cggagacacc gagcaggatt
     4261 cgcacctggc gttgcctcgc ctcactcact cgctcgaccg actcctcggg gtcgagctcg
     4321 tcccgttcca agccgcggtg cgcgctgcga ttccgtccgt aatgagcgcg cacgtcctct
     4381 tctcggcgct cgacccgaac tacccggcca cactgagccc cctcgttttg aaagggctct
     4441 tgcgcgagcg actgggctac accggactcg tcgtgagcga tgacctcgaa atgaatgcca
     4501 tcgtcgaaca ttacgggatc gaaaccgcca tttttcgggg acttctcgcc ggtgtcgact
     4561 gctttctcgt gtgtcacact ccagagc
//
LOCUS       DASTKS010000517         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_82004,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000517 DASTKS010000000
VERSION     DASTKS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82004"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFQ35_10735"
     CDS             <1..992
                     /locus_tag="VFQ35_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931156.1"
                     /translation="APLEVVLLEVEELKVTFPSEQGAVPAVRGASFALAPGEAAALVG
                     ESGSGKSVTALSLLRLLPSSARIDARRVRFDGRDLATLSEAELRRLRGREIGMVFQEP
                     MTSLNPVFAVGEQIGEVLRLHRALDRRAAESEVIALLGRVGISSPERRAKQYPHELSG
                     GMKQRAMIAMALACQPKLLIADEPTTALDVTIQAQIMALLKDLQEELGMAVLLITHDL
                     GVVAHFAETVHVMYAGKIVERASTRDIFAAPAHPYTRALLGALPRPGNEGRRLEAIPG
                     RVPHPSRLPKGCAFSPRCKFVMDRCPDDQPPLLHASADHVAACWLLEDEPNDR"
     gene            982..1995
                     /locus_tag="VFQ35_10740"
     CDS             982..1995
                     /locus_tag="VFQ35_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931157.1"
                     /translation="MTAEAAARREPAAPLLRVRGLRKHFPVRGGVFGNVHGWVKAVDG
                     VSFELARGETLGLVGESGCGKTTVGRSILRLIDAEAEELSFDGEDLLKASRKRLRELR
                     TRMQIVFQDPYSSLNPRMPVGEAVAEPIRAHRLAEGKAVDRRVAELFERVGLPAAYRS
                     RFPHEFSGGQRQRIGIARALALGPKFIVCDEAVSALDISIQAQILNLLRELQSELGIA
                     YLFITHNLNVVRHVADRVAVMYLGEIVELANTDVLFDAPKHPYTRALISANPLPDPTV
                     PLAPLLLKGEIPSPLNPPSGCRFHTRCPEVFEPCPTVIPATTLLREKNEHRVHCHLYT
                     DAP"
     gene            1985..>2943
                     /locus_tag="VFQ35_10745"
     CDS             1985..>2943
                     /locus_tag="VFQ35_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9931158.1"
                     /translation="MLRSVAAALAVLFAGLLLVGLTFSASARQRADFTFVNNAEPKTL
                     DPTTMTGEPEGRLAAALFEGLMRLDAKSLEPVPGAAERYEVSEDGRRYEFHIRKDARW
                     SDGHPVTAHDFAYAWRRLQDPKLGSEYAYILHVVDGAEAFNTYAAQANALVGEVAGRL
                     RELAAQHPNDVPARTFRDFVEQNQLSSRLKGTPDPWLARRLADTHANVSTAELARFAA
                     AIAREAERRRSEAAAANEHFGVDRGVFAKDEKTLVVELRAPTPYFLELTAFHTSYPVP
                     RWVAEAPGNQNDWFLPKKIVSNGAFRMAEWQVGDHIRLVRSET"
BASE COUNT          506 a         1012 c          932 g          493 t
ORIGIN      
        1 aagctccgct cgaagtcgtg ctgctcgagg tcgaagagct gaaggtcacg ttcccctcgg
       61 agcaaggcgc cgttccggca gtgcgcggtg cgagctttgc cctggcgccc ggagaagcag
      121 cggccctcgt gggcgagtcg gggtctggca aatcggtcac ggccctctcg ctgctcaggc
      181 tcttgccgag cagcgcgcgc atcgacgccc gccgcgtgcg attcgacggg cgcgatctcg
      241 ccacgctgag tgaagccgaa ctgcgtcgcc tacgaggccg cgagatcggc atggtgtttc
      301 aagagccgat gacctcgctg aacccggtgt tcgccgtggg cgagcaaatc ggcgaagttc
      361 tgaggctgca ccgtgccctc gaccgacgcg ccgcggagag cgaggtcatt gcgctgctcg
      421 gtcgcgtcgg gatttcttct cccgagcgcc gcgccaagca atacccgcac gagctctcgg
      481 gcggcatgaa gcagcgcgcg atgatcgcga tggcgctcgc ctgccagccg aagctgctca
      541 tcgccgacga gccgacgacc gcgctcgacg tcaccattca agcccaaatc atggctcttc
      601 tgaaggacct tcaagaagag ctcggcatgg ccgtgctgct cattacgcac gacctcggcg
      661 tcgtcgcgca cttcgccgag accgtgcacg tgatgtacgc cggcaagatc gtcgagcgcg
      721 ccagcacacg cgacatcttc gcggcgcccg cgcaccccta tacgcgcgcg ctgctcggcg
      781 ccttgccacg cccaggcaac gaggggcgaa ggctcgaggc aattcccggt cgcgtgccac
      841 atccgtcgcg tttaccgaag ggctgcgcat tttctccccg ctgcaagttc gtgatggatc
      901 gctgccccga cgatcaacct ccgttactac acgcgtccgc tgaccacgtc gcggcatgtt
      961 ggctcttgga agacgaaccg aatgaccgct gaggccgctg ctcgacgcga accggctgcg
     1021 ccgctcttac gtgtgcgcgg tctccgcaaa catttcccgg tgcgcggggg tgtgttcgga
     1081 aatgtgcatg gatgggtgaa ggcggtggac ggcgtctcgt tcgagctcgc gcgcggtgaa
     1141 acgctcgggt tggtgggcga atcgggctgc ggcaaaacga cggtgggccg ctcgatcttg
     1201 agattgatcg atgcggaagc cgaggagctc agcttcgacg gagaagatct gctcaaggcc
     1261 tcacgcaagc gcctgcgaga gctccgcacg cgcatgcaaa tcgtgtttca agatccgtac
     1321 tcgtcgctga acccgcgcat gcccgtgggt gaagccgtcg ccgagccgat tcgcgcgcac
     1381 cgactcgcgg agggcaaagc cgtcgatcgc cgcgtcgcgg agctcttcga gcgcgtgggg
     1441 ctacccgcgg cttaccggtc gcgcttccct cacgagttct cgggcgggca gcggcaacgc
     1501 atcggcatcg cgcgggcgct cgccctcgga ccgaagttca tcgtgtgcga cgaagcggtg
     1561 tccgcgctcg acatctccat tcaagcgcaa atattgaatc tgctgcgcga gctgcagagc
     1621 gagctcggca tcgcgtactt gttcatcacc cataacctca acgtggttcg gcacgtcgcg
     1681 gaccgcgtgg ccgtcatgta cctgggcgaa atcgtcgagc tcgcgaacac ggacgtgctg
     1741 ttcgacgccc cgaagcatcc ctacacgcgg gcgctgattt cggcgaatcc cctgcccgac
     1801 cccaccgtgc cgctcgcgcc gttgctcctc aaaggcgaaa ttccctcgcc gctcaacccg
     1861 ccgagcggtt gccgttttca cacgcgctgt cccgaagtct tcgagccgtg ccccacggtg
     1921 atccccgcga cgacgctctt gcgcgagaag aacgagcaca gggtgcactg ccacctctac
     1981 accgatgctc cgtagcgtcg ccgccgccct cgccgtgctc ttcgccgggc tcttgctcgt
     2041 cgggctcacg ttctcggcgt cggctcgcca gcgcgcggat ttcaccttcg tcaacaacgc
     2101 cgagccgaag accctcgacc cgaccacgat gacaggggag ccggagggtc gcttggcggc
     2161 ggccctgttc gaaggcctga tgcgcctcga cgcgaagtca ctcgagccgg tgcccggcgc
     2221 cgccgagcgc tacgaggtct cggaggatgg acgccgctac gagttccaca ttcgcaagga
     2281 cgcacgctgg agcgacggac atcccgtcac cgctcacgac ttcgcctacg cctggcggcg
     2341 gctgcaagat cccaagctgg gctccgagta cgcctacatt ctccacgtcg tggatggcgc
     2401 ggaggcgttc aacacgtacg ccgcccaagc aaacgccctc gtcggtgagg tcgctggtcg
     2461 actacgcgaa ctcgcggcgc aacacccgaa cgacgtgccg gcgcgcacct ttcgcgattt
     2521 cgtcgagcag aatcagctgt cctcacgact gaaaggcacc cccgacccct ggctcgcgcg
     2581 gcgcctcgcg gacacgcacg cgaacgtcag caccgcagag ctcgcgagat tcgccgcagc
     2641 catcgcgcgc gaagccgagc gccggcgcag cgaagccgcg gccgcgaacg agcacttcgg
     2701 cgtcgaccgc ggcgtcttcg ccaaggacga aaagacgttg gtggtcgagc tgcgcgcgcc
     2761 cacgccgtac ttcttggagc tcaccgcttt tcatacgagc tacccggtac cgcgctgggt
     2821 cgccgaagcg cccggcaacc aaaacgactg gttcttgccg aagaagatcg tctcgaacgg
     2881 cgcgttccga atggctgagt ggcaagtggg cgatcacatt cgcttggtga gaagcgaaac
     2941 cta
//
LOCUS       DASTKS010000518         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1867045, whole genome shotgun sequence.
ACCESSION   DASTKS010000518 DASTKS010000000
VERSION     DASTKS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867045"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..436
                     /locus_tag="VFQ35_10750"
     CDS             116..436
                     /locus_tag="VFQ35_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9931159.1"
                     /translation="MHDLHDSRHNTVAVWDFRGKGPEGTQPIQLQAHHGVCTKLAFSP
                     HTGVLASGSQDTTVVLWQPRKHARPVQFAFLSDEVTALEWHPEHETLFGADASGSIVA
                     WPVS"
     gene            complement(463..891)
                     /locus_tag="VFQ35_10755"
     CDS             complement(463..891)
                     /locus_tag="VFQ35_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9931160.1"
                     /translation="MKAPLPRDEQIRELLTSRGLRITDQRVALLRELAKVKAPISHPE
                     LTERMVGSGLDRATVYRNLLTLADAGVLVRAQLGDQVWRYELPRTMSSDHAHHPHLVC
                     SECGNVRCLPAGTVKLHGEAAKSEIAEVQLRGRCASCVGG"
     gene            1180..1560
                     /locus_tag="VFQ35_10760"
     CDS             1180..1560
                     /locus_tag="VFQ35_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931161.1"
                     /translation="MLLLLLPDVFRLGHLVLVRHATCPYDGALIHDERETVGVDPSPT
                     GSRLPPEQAALSAPHHHEERCGLSSGAHRAVATLRFEQSLVATVSPPPDPLVAAREEP
                     SVRGVLSYAPKISPPARAQARTVA"
     gene            2041..2349
                     /locus_tag="VFQ35_10765"
     CDS             2041..2349
                     /locus_tag="VFQ35_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931162.1"
                     /translation="MATWTPLLSTVGLGLVLTEAAHGALVVAAIAVSLTTAGLRARRL
                     GTPTPLLFALAGAFLLALAHWHGASLTLSASAVVAFVLSAVVGRRASGIVPAVRREPV
                     "
     gene            2349..>3094
                     /locus_tag="VFQ35_10770"
     CDS             2349..>3094
                     /locus_tag="VFQ35_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9931163.1"
                     /translation="MLGFDPAAHALRVMWSASVGDYITALDVDSTGELLALGTDSGEV
                     CVFDVTSSALRTRVTVHDGGVLSAKWSPTQRLLATSGQDGSLRLQDSDGNERCRQPSS
                     RWIERLAWSPDGTRLAAASGSVVRIFDAEGRAMLATETHPGTVTGLAWNRRGNQFASA
                     CLDGVRIFHGDSPTRSRGLRWRVPLVSLSWSPNDAIIAAVTEANTVHFWRLPSGRDAE
                     IAGFPARPRALAWDPASHFLATSGDAKVAV"
BASE COUNT          497 a         1061 c          998 g          538 t
ORIGIN      
        1 gccagcgatc acccagagcg gcgcgccgcg cctcgtcctc acgctgtcga ccgcgaatcc
       61 gagatgacca ccgtgctgcg gtcgcaacgc cgccgccgag tacatcgcaa tccggttgca
      121 tgacctgcat gactcacgcc acaacacggt ggcagtgtgg gactttcgcg gcaaagggcc
      181 cgagggaaca cagcccatcc aactccaagc tcaccacggc gtgtgcacga agctcgcctt
      241 cagcccgcac actggcgttc ttgccagcgg gagccaagac accacggtgg tgctctggca
      301 gccgcgcaaa cacgctcgcc ctgtccagtt cgcgttcctg agcgacgagg ttaccgcgct
      361 cgaatggcac cccgagcacg agacgctctt cggagcagac gcgagcggga gcatagtcgc
      421 gtggcccgtg agctgagagc ggagccgaga attacggcgg cgtcatccgc ctacgcacga
      481 cgcgcagcgg ccgcggagct gcacctccgc gatttcgctc ttggccgcct cgccatgcaa
      541 tttgacggtg ccggcgggta ggcagcgcac gttcccacac tcggagcaaa cgagatgagg
      601 atggtgcgcg tggtccgagc tcatcgtgcg cggaagctcg tagcgccaga cctgatcgcc
      661 gagctgagcg cgcaccaaga ctccggcgtc cgccaaggtc agtaagttcc tgtatacggt
      721 cgcgcggtcg agaccgctgc caaccatccg ctccgtgagc tctggatggc tgatcggcgc
      781 cttgaccttc gcgagctcac gaaggagggc aacgcgctga tcggtgattc gcaacccacg
      841 cgaggtcaaa agctcgcgga tctgctcgtc tcgaggcaag ggcgccttca ttcaccggag
      901 catcccttgt tcgtcgccga cttgcaaccc gagcgaatcc acgccctgga cacggcccgg
      961 gcgccggctc cgaactgttc gtaatcaccg gcatttgccc catctgtgac cccgcgcgca
     1021 acgcgcacgc atgcaatccg gttgcattta ccttgacgcc acgatgcgcg ctgcgccaca
     1081 ttcgcgtgca gcaagttgca gagattggac caagcgagtc gtcccatacg gcgggcgcgt
     1141 ttcagcagga ggctctgggc gctggtcgca gcgtggctga tgctccttct gctgctgccc
     1201 gacgtgttcc gcttgggaca cctcgttctc gttcgtcacg ctacctgtcc atacgacggc
     1261 gccctgattc acgacgagcg cgaaacggtc ggagtcgacc cgagtccgac aggctctcgc
     1321 ttgccgcccg agcaggcggc cctgagcgct ccgcaccacc acgaagaaag gtgcgggctg
     1381 tcgtccggcg ctcatcgtgc cgtcgcgacg ttacgcttcg agcaatcgct cgtcgcgacc
     1441 gtgtcgcccc cacctgaccc gctcgtcgcg gcacgtgaag agccttccgt gcgcggcgtt
     1501 ctgtcgtacg ctcccaaaat ctcgccaccc gcgcgggcgc aggcgcgaac ggtcgcctga
     1561 ataggcggtt tcttctgcgc cagagtcgtc tagtcgagcc ctcttgaagt gggctcctgc
     1621 gccttgcttc gctcgcgtcg cgagaacgat gcacgcgggc tctggcgcgc acgatgcgtc
     1681 gccgcgcgac agcgtgccgc gcgggctttc gcgtcggctg cgccttcgcg cgtcgccaaa
     1741 ataactacga attgtcggct cgatttgccg cgcgcgagac gccccgaggc cgcgcgctgg
     1801 cgacaggtcg agccagaaac cggcgcccgc gttcccctcg agcggtcgtg cgcgcgcggg
     1861 agaagggtct ccatgaaaat cagccatctc gaacctacgc acgaagaatg caactgcccc
     1921 cgtcaccgac gaaacagcga aacggaccgg aaccgagcgc acgcgaaatc cccgcgacag
     1981 cgtttgcgct ggtccggcac gctgctctct attgcggctt gcgtattctg ccctgtctgc
     2041 atggccacgt ggacgccgct gctctccacg gtcgggctcg ggttggtgct cacggaagcc
     2101 gcgcacggcg ccctcgtcgt cgcagcgatc gccgtttcgc tgacgacggc cggcctgcgc
     2161 gctcgccggc tcggcacgcc gacgccgttg ctcttcgcac tcgcgggtgc attccttttg
     2221 gccctggcgc actggcacgg cgcgagtttg actctctctg cttcggctgt tgtggcgttc
     2281 gtcctctctg ccgtggtcgg tcgacgcgcg agcggtatcg ttcccgcggt tcgacgggag
     2341 ccggtgtgat gcttgggttc gatccggcag cgcacgcgct gcgggtgatg tggagcgcca
     2401 gcgtaggtga ctacatcacc gcgctcgacg tcgactccac gggagagctc ctggcgctcg
     2461 gaaccgactc cggggaagta tgcgttttcg acgtcacctc gagtgcgctg cgcacgcgcg
     2521 tgacggtgca cgacggcggt gtgctctccg cgaagtggtc accgacccag cgcctgctcg
     2581 cgacatcggg ccaagacggc tccctccggc tgcaagattc ggatggcaac gagcgctgtc
     2641 ggcagccgag ctcgcgttgg atcgaacgac tggcctggtc accggacggg acgcggctcg
     2701 ccgcggcgtc ggggagcgtg gtccggatct tcgacgctga aggccgagca atgctcgcaa
     2761 ccgaaacgca cccaggaacc gtgaccggtc tcgcttggaa tcgacgcgga aaccaattcg
     2821 cgagcgcgtg tttggatggg gtccggatct ttcacgggga ctcgccgact cgttcgcggg
     2881 gtctgcgctg gcgcgtaccg ctggtttcgc tcagctggag tccgaatgac gcgatcatcg
     2941 ccgcggtcac cgaggcaaat acggttcact tctggagact cccgtccggt cgcgacgccg
     3001 aaatcgcggg atttccggcg agaccgcgcg cgctggcgtg ggaccccgcg agtcacttcc
     3061 tcgcgacgag cggcgacgcc aaggtcgcgg tgtg
//
LOCUS       DASTKS010000519         7322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_973821, whole genome shotgun sequence.
ACCESSION   DASTKS010000519 DASTKS010000000
VERSION     DASTKS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973821"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VFQ35_10775"
     CDS             complement(<1..1630)
                     /locus_tag="VFQ35_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9931164.1"
                     /translation="MIVDGVNITARLGESQALSVLSDLGHAVSALSRGKRDRATLPLY
                     AADQAWELGLEADGSDVLVSVYRTGPAPEVAVHERRVDLVALRAALVRAIDEARTREP
                     SGGALGGLAAARRLLEAPWPSYGRRPLERVTALVSPKPAGGLTFGAQAAFRTSGESDF
                     ASGSPHVERADLHALLVRGSVVASARGRALRVDDVSPFLVAERLLTLAEEVLEAWRGE
                     RALVRRVNIDGAWVGVQRGPGDGPLTLTLSARGSSRDEGGVALAGLKPPSVVKAIARF
                     ARCLYDKFIELDRSQAKNLRLVELGRAAAELDSAVADATVDDSLTNPEPESYRSFGLP
                     RVRAERGLWEHSGKMRFLPRWVATVPGIDLRATFHCGDRIVVGSQRETACLESNTGKL
                     LWRVPGQRAACVATPLGIARIHPDGLLELLDIDCGEARFSTRLRPRSVSGAAGALVNA
                     PGLPRLLVLAEGDRSVTAVDLVSGEVRWRYSARRPTTYRLRRAGKLLLAAGGDSALVA
                     LDVVTGEVVWRVRDRLPFTGDLCVDKDSAFAIAGG"
     gene            1903..3480
                     /locus_tag="VFQ35_10780"
     CDS             1903..3480
                     /locus_tag="VFQ35_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931165.1"
                     /translation="MRDTHPSTKWLYTLFIGAAVVIAPMVWFWEFTVDDAWITGRVAH
                     HIATGRGYRFNPDGPVVDAVTPLGWAFVLAPFAASGPGAALVAAKWLGALSWLGAALS
                     LFAKLARLTWRAIIIAALLIATSMPIAAWAVAGMETGVVLALATLGLGEGWTARLALG
                     LAAGLRPELLPWAFIVGSARSALMTGTALDRARRVALASVPLLLPFLVVAVTRTVIFG
                     AAYPLAVLAKPSDASSGLRYALGALAFTGAAWTSLGIRSLAALRATPKSLPSPDGRSA
                     SHSFVAPPRRTSVRLVVSPRTARVSLPGSVQDLGVALKPFTRVLVAAALVHALVLVAV
                     GGDWMPIYRLFVPVLPSLVLAGAELAQTEPLWKVVMRSGVALFAAALLVLARGQAVRG
                     VGVQRAELIHEARPLLASARRIATLDVGWVGAATPAHIVDLAGVTDPTVARLPGGHTT
                     KRLPDSFLESRDIDALVLLADYAGTWPNHHFARVVEARIVTLPGAERFRAVGWVPLLG
                     TRQAYVVARRMSEAQAD"
     gene            complement(3468..5159)
                     /locus_tag="VFQ35_10785"
     CDS             complement(3468..5159)
                     /locus_tag="VFQ35_10785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931166.1"
                     /translation="MQRRATQGKTPASRLKTLYLHLLNEHGSAAADAFLLSTRLDRDF
                     LSDETRLVPIELWHTALGAFASRWGRDSLPTMARAAVHPENLGVWTHVLRGATDIASA
                     YRRLDHFGGEHVWTERWRTISERPGYWRGVLRAPSDAGLERDGLFSLAREAELASVPL
                     LFGAPKATVLALPSTGSNGSQEFEVRWREPNATQTTLLGLLGGAALGAVWNFLANGRD
                     WITGSLLGGACVGVVALLAAHESRRRAQSTAQLMRIQALERAATLREAREQGAVGFHA
                     GQVVAGQYRLTEKLGVGANGTIWEAERIADGGLVAIKLLRAAVAHDNIAADRLRREAA
                     ALGLAWHPNVVEIYDDGHLPDGTSYLVMERLYGESLAARLRRKGMLTPREVLPIALEL
                     CDALGAVHAAGIVHRDVKPSNIFIATEPATHDAPASPEHAKILDFGVARVEWAETRLT
                     NADVPLGTPGYMPPEQIEGQEVDARSDVYALGAALYECLSGRAPPARSGDSWEASSEE
                     STGESGVQPALRAIPGEWRAIIERAMSPLPRDRFQDTRALREALLATPEARLQSA"
     gene            5301..5798
                     /locus_tag="VFQ35_10790"
     CDS             5301..5798
                     /locus_tag="VFQ35_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET9931167.1"
                     /translation="MEVFNPAAIRRFAWIGIAACSLGLGAHSAQAGTLAVVAGETLDI
                     KADSLEVDMARGSALLEGKVKATLGELQVECARVEVRYDEAPNVRWARGTGGVSARLK
                     GIAATANTVEVDVARRSVRLEGAVKLSRGKGWVTADTATIDVATRKVVLNDVQGSIPV
                     QTPAR"
     gene            6094..>7322
                     /gene="rpoN"
                     /locus_tag="VFQ35_10795"
     CDS             6094..>7322
                     /gene="rpoN"
                     /locus_tag="VFQ35_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET9931168.1"
                     /translation="MTPQLQQAIRLLQLSRLELIEEVRKELDNNPVLSDEESSERTAR
                     AEPGEPRAAAEPQLEDPGSYYNKTNEDQKASERATREVDWEKFLENRTLQQAPGTSRG
                     GFEELPPIEQNLTKPRNLPDHLLWQLQMSDFNEPERKFAELVIGNLDEKGYLDLAGVE
                     RPDGTRTPDLTIEDLAEEAGLHPDDAPLVLEMIQKFDPVGVAARDLRECLLIQAYAAG
                     YEDAETVTEIIKNHLHQLERHNYQAIARDMKLAIEDVYEAAKEIQDFESRPARNFTDQ
                     DERTIGITPDVYVIKDGSDFVVLDNDRGVQRLFINEALTKRMMKDPSAKEFIGEKLRN
                     AQWLIRAIEQRRKTIIRVAECIVEKQRDFFERGVSFLKPMILRDVAEAVGMHESTISR
                     VTTNKYMHTPQGLFELK"
BASE COUNT         1244 a         2461 c         2410 g         1207 t
ORIGIN      
        1 ggccgccggc gatcgcgaac gcactgtctt tgtcgacgca cagatcgccg gtgaacggca
       61 gccgatcacg gacgcgccaa acgacttcac ccgtgaccac gtcgagcgcg acgagggccg
      121 agtctccacc cgccgcgagc agcagcttgc ccgcccgacg caaccggtac gtcgtcgggc
      181 gtcgcgcgct gtagcgccag cgcacctcac cagagacgag atcgacggcg gtgacggagc
      241 ggtcgccttc ggctagcacc aaaaggcgag gcaaccccgg cgcgttgacg agcgctccgg
      301 cggccccgga tacgctgcgc gggcgaagcc gcgtcgagaa gcgcgcctcc ccgcagtcga
      361 tgtcgagcag ctccaagagg ccgtccgggt ggatgcgggc gatcccgagc ggcgtcgcga
      421 cgcacgcagc gcgctgcccc ggcacgcgcc agaggagctt gccggtgttg ctctcgaggc
      481 acgccgtttc gcgctggctt ccgacgacga tgcgatcccc gcagtggaaa gtcgcgcgca
      541 gatcgatgcc gggcaccgtc gccacccagc gcggcaaaaa gcgcatcttg ccgctgtgct
      601 cccagagccc gcgctcggct cgcacgcgtg gcagcccgaa agaacgatag ctttcgggct
      661 cggggttcgt gagcgagtcg tcgacggtag cgtccgcgac cgcgctgtcg agctcggcgg
      721 cggcgcgacc gagctccacg agacgaagat ttttcgcttg gctgcgatcg agctcgatga
      781 acttgtcgta gaggcagcgc gcgaaacgcg cgatcgcctt cacgacgctc ggtggcttga
      841 gcccggcgag cgccacgccg ccctcgtcgc ggctcgaacc gcgcgcgctc aaggtcaacg
      901 tgagcgggcc gtcgcccggg ccgcgctgca cgccaaccca agcgccgtcg atgttgacgc
      961 ggcgcacgag agctcgctct ccgcgccacg cctcgagcac ttcttcggcc agcgtgagca
     1021 gccgctccgc caccaagaac ggagaaacat cgtcgacgcg cagagcgcgt ccccgcgccg
     1081 aggccacgac gcttccacgc accaagagcg cgtgaagatc cgctcgctcg acgtgcggcg
     1141 agccagacgc aaagtcgctc tctccactcg ttcgaaacgc tgcctgtgca ccgaaagtga
     1201 gtccacccgc gggcttcggt gacaccagcg ccgtcacacg ttcgagcggg cggcgcccgt
     1261 agctcggcca cggagcttcg agcagtcgcc gggccgcggc gagcccgccc agcgcgccgc
     1321 cggacggctc tcgcgtgcgc gcctcgtcga tcgctcgcac gagcgcggct cggagcgcga
     1381 cgaggtcgac tcggcgctcg tgaaccgcga cctcgggcgc gggcccggtg cgatacacgc
     1441 tcacgaggac gtcgctgccg tcggcctcga gcccgagctc ccaagcctgg tccgcggcgt
     1501 agagcggcag cgtcgcgcga tcgcgcttgc cgcggctcaa cgccgagacc gcatgcccga
     1561 ggtcggacag cacggacaaa gcctggctct caccgagccg agcagtgatg ttcaccccat
     1621 ccacaatcac atcgaacagc ccgtgaagcg gtgcgagtgc ggtggaaggg ggggcctggg
     1681 gccgtgggcg gacgacaacc tcgatggctg acatcagacg ttgctcatcg tagatagccc
     1741 gccgcacatt cctaagtttt gcgaaccgat cgctcaagag tggacacccg tcgccgacct
     1801 agctagccgt gacggattcg gtcgggccgg tctcttccga atggcgctgc ctcctcgttc
     1861 gagcctgaat ttgggcaatt cttagggccc cccagcggct tcatgcgcga cacgcacccg
     1921 tcaacaaaat ggctctacac cctattcatc ggcgcggcgg tggtgatcgc tccgatggtt
     1981 tggttttggg agttcacggt cgacgacgcg tggatcaccg gacgcgtggc ccatcacatc
     2041 gcaaccggtc gcggctatcg gttcaaccct gacggacccg tggtcgatgc ggtgacgccg
     2101 cttggttggg cgttcgtcct cgcgccgttc gcagcgagcg ggccgggggc agctttggtt
     2161 gcggccaagt ggctcggagc gttgagttgg ctcggagcgg cgctgtcgtt gttcgcgaag
     2221 ctcgctcgct tgacgtggcg cgccatcatc atcgccgcct tgctcatcgc gacctcgatg
     2281 ccgatcgcgg cgtgggcagt cgccggcatg gagaccggcg tcgtcctcgc gctcgcaacc
     2341 ctcggcctgg gggaagggtg gaccgcgcgt ctggcgctcg gcttggcggc ggggctcaga
     2401 ccggagcttc tgccgtgggc gttcatcgtg ggcagcgcac gctccgcgtt gatgacgggc
     2461 acggcgctcg atcgagcgcg ccgagtcgcg ctggcgagcg tcccgttgct cctgccgttc
     2521 ctggtcgtgg ccgtgacgcg cacggtgatc tttggcgccg cctatccgct cgccgtgttg
     2581 gccaaacctt ccgatgcgag ctccggcctg cgctacgcgc tcggcgcgct cgctttcacg
     2641 ggcgcggcgt ggacctccct cggcattcgt tctctcgcgg ctctaagagc cactcctaaa
     2701 tccctcccgt cgcccgacgg cagatccgct tcgcattcct tcgttgctcc tcctcggcgt
     2761 acttcagtac gcctcgtcgt ctcgcctcgg actgctcgcg tctctttgcc gggctcggtc
     2821 caggatttag gagtggctct aaagccgttc acgcgggtcc tcgtcgcggc cgcgctcgtg
     2881 catgcgttgg tgctggtagc ggtcggcggc gattggatgc cgatttaccg gctcttcgtt
     2941 cctgttttgc cgagcctcgt cttggcaggc gcggagcttg cgcaaaccga gcctctctgg
     3001 aaggtagtga tgcgcagcgg agtggccttg ttcgcggcgg cgctgctcgt gctcgcgcgt
     3061 ggtcaggcgg tacgcggcgt tggggtgcag cgcgccgagc tcattcacga agctcggccg
     3121 cttcttgcct cagcgcgacg catcgcaacg ctcgacgtcg gttgggtggg tgcggcaacg
     3181 cccgctcaca tcgtcgatct cgcgggagtg acggatccga cggtcgcccg gctacccgga
     3241 gggcatacga cgaaacgcct tccggatagt ttcctcgagt cccgagacat cgacgcgctc
     3301 gtgctcctcg ccgattacgc gggcacctgg cccaatcatc acttcgcgcg ggtggtcgag
     3361 gctcggatag tgaccctgcc aggcgcggaa cgcttccgag ccgtgggatg ggtgccgctg
     3421 ctcgggacgc gccaagcgta cgtcgtggcg cgccggatga gcgaagctca ggccgattga
     3481 agtcgcgctt cgggcgtcgc gagcaacgct tcgcgcagcg cgcgagtatc ctgaaagcga
     3541 tctcggggca ggggagacat cgcgcgctcg atgatcgcgc gccattcccc ggggatcgcg
     3601 cgcaacgcgg gctgcacgcc gctttcccca gtcgattcct ccgacgacgc ttcccacgag
     3661 tcgcccgacc gagccggcgg cgcgcgcccc gaaaggcact cgtacagcgc cgcgccgagc
     3721 gcgtagacgt cgcttcgcgc atccacctct tggccttcga tctgctccgg cggcatgtag
     3781 cccggcgtgc cgagcggcac gtccgcgttg gtgaggcgcg tctcggccca ctccacgcgc
     3841 gccacgccga agtcgaggat ctttgcgtgc tcaggagaag ccggcgcgtc gtgagtcgcc
     3901 ggttcagtcg cgatgaagat gttgctcggc ttcacgtcgc gatgaacgat gcccgccgcg
     3961 tgcaccgcgc cgagcgcgtc gcagagctcg agcgcaatcg gcaagacttc acgcggcgtg
     4021 agcatgccct tgcggcgtag gcgagccgcc aacgactccc cgtagagccg ctccatgacg
     4081 aggtaactgg tcccatccgg gaggtgcccg tcgtcgtaaa tctcgacgac gttcgggtgc
     4141 caggcgagac ccaacgccgc agcttcgcga cgcagtcgat ccgccgcgat gttgtcgtgc
     4201 gcgaccgcgg cccggagcaa tttgatggcg acgaggccgc cgtccgcgat gcgctcggcc
     4261 tcccaaatcg taccgttcgc accgaccccg agcttttcgg tgaggcgata ttgccccgcg
     4321 actacctgac cggcgtggaa gccgaccgcg ccctgttcgc gagcctctcg gagcgttgcc
     4381 gcacgctcga gcgcctgaat gcgcatcaac tgggctgtcg actgagctcg gcgacggctc
     4441 tcgtgagccg cgagcaacgc caccacgccg acgcacgcgc cgccgagcaa cgaccccgtg
     4501 atccaatcgc gcccgttcgc gaggaagttc cacacggcgc cgagcgccgc gccaccgagc
     4561 aagccgagca gggtcgtttg cgtagcgttc ggctcgcgcc agcggacctc gaattcctgc
     4621 gaaccgttcg acccggtgct cggcagagcg agcacggttg ccttcggcgc accgaaaagg
     4681 agcgggacgc tcgcgagctc cgcctcgcgc gccaacgaga agagcccatc gcgttcgagg
     4741 cccgcgtcag aaggggcccg cagcacgccg cgccagtaac cagggcgctc gctgatggtg
     4801 cgccaacgtt cggtccagac gtgctcgcca ccgaaatgat cgagacgccg atacgccgac
     4861 gcgatgtccg tcgccccccg aagcacgtgc gtccagactc cgaggttctc cgggtgcacc
     4921 gcggcgcgcg ccatcgtggg aaggctgtcg cgaccccaac gcgacgcgaa cgcgccgagc
     4981 gcggtgtgcc agagctcgat cggtacgagt cgcgtctcgt cgctcagaaa atcgcgatcg
     5041 agccgagtgc tcagtaggaa cgcgtcagcc gccgccgacc cgtgctcgtt cagaaggtgc
     5101 aagtacagcg tcttcagacg tgacgctggc gtcttccctt gcgttgcacg acgctgcacg
     5161 taacaaagcc tcccggacct agagggcgcg gtcaagcacc cttcgttctt cgtggcgcac
     5221 accggttgaa acgatcacga cactcaaact tggggaggct atcactgccg cccgacgtag
     5281 ctcatgctaa ggtaaacgcc gtggaagttt tcaatcccgc agcaatacgc aggttcgcct
     5341 ggattggcat cgccgcgtgc tcgttgggct tgggagctca ttccgcccag gccggtacgt
     5401 tagccgtggt cgctggcgaa accctcgata tcaaagcgga ctcgctcgag gtggatatgg
     5461 cccgaggctc agcgctgctc gagggcaagg taaaggcgac cttgggcgag cttcaggtcg
     5521 aatgtgcgcg agtcgaagtg cgctacgacg aggcaccgaa cgtgcgctgg gctcgaggga
     5581 ccggcggcgt gagtgctcgg ctgaagggca tcgcggcgac ggcgaatacg gtcgaggtcg
     5641 acgtcgcgcg ccgaagtgtc cgcctcgaag gtgcggtgaa gctcagccgc ggaaaaggct
     5701 gggttaccgc cgataccgcg acgatcgacg tcgccacgcg caaggtcgtg ttgaacgacg
     5761 tgcagggctc gatccccgtc caaacgcccg cgcggtagtc gaaactcgag cgccgtgttt
     5821 cgtcatcgct cgcggtcgtt cgaacggcac tcaggggacg gtcggttttc gccgagtggc
     5881 cgcgcatgcg ccacacttga atgatttccg ccacagcgtt cggatctgat ttggtcgagt
     5941 ggcccatcaa atcgacccaa aacgtgagct gggggaggcg cgctttttgc tttggggtgt
     6001 tagactgccc tcaactccgg tcattgccgg ctgtggagcg tacgggtcgc gatggagatt
     6061 aagcagcagt tacggctgtc ccagcagcta gtcatgactc cccagttgca gcaggcgata
     6121 cgcttgctgc agctctcccg actcgagctc atcgaggaag ttcgcaagga gctcgacaac
     6181 aacccagtcc tctcggacga ggagtcgtcg gagaggacgg cgcgggcgga acctggcgag
     6241 cctcgcgcag ccgcggagcc gcagctcgaa gaccccgggt cgtactacaa caagacgaac
     6301 gaggatcaga aggcgagcga gcgtgcgacg cgtgaggtcg attgggagaa gtttctcgag
     6361 aatcggaccc ttcagcaggc tccgggcacg agccgtggcg gcttcgaaga gctgccgcca
     6421 atcgagcaga acctaaccaa gccgcggaat ctgccggatc atctgctctg gcagctgcag
     6481 atgagcgatt tcaacgagcc ggaacgcaag ttcgccgagc tcgtcatcgg taacctcgac
     6541 gagaagggct acctcgacct cgccggggtc gaacgccctg acggcacgcg cacgccggat
     6601 ctcaccatcg aggacctcgc cgaggaggcg gggctccacc cggacgacgc gccgctcgtc
     6661 ctcgagatga tccagaagtt cgatccggtc ggggtcgcgg cccgagacct gcgcgaatgc
     6721 ttgctgattc aggcgtacgc ggctggctac gaagacgccg agaccgtcac cgagatcatc
     6781 aaaaatcacc ttcaccagct cgagcggcac aactaccagg ccatcgcccg ggacatgaag
     6841 ctcgccatcg aggacgtcta cgaggcggcg aaggagattc aggatttcga gagccgtccg
     6901 gcgcgcaatt tcaccgacca ggacgagcgc acgatcggga tcacgcccga tgtttacgtc
     6961 atcaaagacg gctccgactt cgtcgtgctg gacaacgatc gcggcgtgca acggctattc
     7021 atcaacgagg cgctcaccaa gcgcatgatg aaagatcctt cagccaaaga gttcattggt
     7081 gagaaattgc ggaatgcgca gtggctcatc cgcgccatcg agcagcgccg caagaccatc
     7141 attcgagtcg ccgagtgcat cgtcgagaag caacgcgact tcttcgagcg cggcgtctca
     7201 ttcttgaaac ccatgatctt gcgcgacgtg gccgaggcag tcggcatgca cgagtcgacg
     7261 atctcgcgcg tcacgaccaa caagtacatg cacacgcccc aagggctgtt cgagctcaag
     7321 ta
//
LOCUS       DASTKS010000520         4686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_750248, whole genome shotgun sequence.
ACCESSION   DASTKS010000520 DASTKS010000000
VERSION     DASTKS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750248"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..2136)
                     /gene="hisC"
                     /locus_tag="VFQ35_10800"
     CDS             complement(217..2136)
                     /gene="hisC"
                     /locus_tag="VFQ35_10800"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9931169.1"
                     /translation="MTDRRVTRAVILAAGTGSRLREGDENELPKPLRPVGGVPLCVRV
                     LRTLEQAGIREAVVIIGYQGERVRKALLSEPSLGLKLTFVENPRFDKKNGVSLLTAAE
                     HIDQECILTMSDHLYSPELVRRLIAWDLPAGACALGVDYDIERCFDIDDATKVVVDRT
                     HIARIGKELEEYDALDTGVFRIGPALIDELRKLDQERGDCSLSDGVYALARRGDFYAV
                     DVGEVRWIDVDTPPALERAEAMLRVFGDSLGDEPGSGAPVSIDPEALEMFAPSWVRAA
                     KPYNESHFAVAESQAGITRMMSNESPYAPSARVVNAIVEAALRGNLYPSGADQLAQRL
                     AAREALSPGSVLLGAGSTELIDVVIRTFVGPGEEVLLSVPTFSMYEARTRTQGGIPVL
                     VPMTDDHEHDIGEMIRAVTERTKVIFLCTPNNPTGGSIPVEDLKRLLRLGLPTVIDEA
                     YYEFGDGHSFAPLIEQFPNAIVLRTFSKAFGLAGLRLGYALSHPAVQRLLSRVKVPWN
                     LPAVTIAAASAILDDEAEQQGRLGSLRDAREELFRRISDIPGLRPVPSEGNFVLIDVS
                     GTGIGAERWVDLMVQEGVLVRSLSVHHASRSWVRVTVGTAEQNLKCVSGLERIVSRST
                     RVRRPFAAPAVSDAE"
     gene            complement(2423..3166)
                     /locus_tag="VFQ35_10805"
     CDS             complement(2423..3166)
                     /locus_tag="VFQ35_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET9931170.1"
                     /translation="MSNSTSPRADAAVILVAGVGSRLRPLTNDRPKALMDIGGETILN
                     RAIRLLIAHGVSEVVLATGYREDQIRAALQFLDAKISYFRNDAYDSTQNSVSLGLCRS
                     ALRGKSFFKLDGDVVFQPDVLTRLSVSRADLAVAVDSQRALDAEAMKVRAQGSVISAF
                     GKEIPLASAKAETIGIERLTNVGGDRVLARIGSAMAAGSRDQYYEHFYSELVAEGAIS
                     AEAVEVGDLPWSEVDTFEDLERARGAVKR"
     gene            3198..4511
                     /locus_tag="VFQ35_10810"
     CDS             3198..4511
                     /locus_tag="VFQ35_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9931171.1"
                     /translation="MQRPQKLESPQGKLAVLLPGMGAVATTFIAGSLLARRGLARPIG
                     AITQTGTIRLGKRTDNRNPKIADFVPLANLNDLVFGGWDLFPDNAYEAATKAEVLESK
                     HLDPIRDELSAIKPMSAVFYPEYVKRLTGSNVKTGKTKADMVEAVRDDVRRFIKQNGC
                     SRAVAVWCGSTEIYLEPRPVHQSVAAFEKGLLENDPSISNSMIYAWALLKEGIPYANG
                     APNLTVDIPAMHELARETSSPTCGKDFKTGQTLMKTILAPGFKARMLGMKGWFSTNIL
                     GNRDGEVLDDPDNFKSKEVSKLGVLEDVLQPHVYPELYGDLYHKVRINYYPPRGDAKE
                     GWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLALFMDLAQRAGFRGVQEWLSFY
                     FKSPMTAPGLYPEHDLFIQSMKLKNTLRWMMGEELITHLGHEYYD"
BASE COUNT          869 a         1558 c         1462 g          797 t
ORIGIN      
        1 ccagacaatc gagaccaccg tgacgaggtt cgggctcacc ggggtcgaga acaacgcctt
       61 cgcgagcacg taggcgagcg gccggtgcac ccagacgtcg atcggctctt cgacctcgag
      121 cggcttcagg ctcttccagt aaccgtccca gaaagccatc tagcgcctag tcgccgagcc
      181 gcgacgagag agctcgccgc ggccgccctt tcaccttcac tccgcgtcgc tcaccgcggg
      241 cgcggcgaac gggcgccgga cgcgcgtcga gcgcgacacg atgcgctcga gaccgctgac
      301 gcatttgagg ttttgctcag ccgtgccgac ggtaacgcgc acccagcttc ggctggcgtg
      361 atgcacgctg agcgagcgaa ctagcacgcc ttcctggacc attaggtcga cccagcgctc
      421 ggcgccgata ccggtaccag agacgtcgat gagcacgaaa ttgccttcgc tcggcaccgg
      481 acggagcccg gggatatccg agatgcggcg gaacagctct tcgcgcgcgt cgcgcagaga
      541 gccgaggcgg ccttgttgct cggcctcgtc gtcgaggatc gcgctcgccg ccgcgatggt
      601 gaccgcgggc aggttccacg gcaccttcac gcggctcagc aggcgctgca cggccgggtg
      661 cgacagtgcg tagcccaggc gaagcccggc gaggccgaac gccttcgaga acgtgcggag
      721 gacgattgcg ttcgggaact gctcgatgag cggcgcgaac gagtgaccgt caccgaactc
      781 gtagtaggcc tcgtcgatga cggtcggcaa accgaggcgc agaaggcgct tcaagtcctc
      841 gaccggaatg ctgccgccag tcggattgtt gggcgtgcag aggaagatga ccttggtgcg
      901 ctcggtgacc gcgcgaatca tctcgccgat gtcgtgctcg tggtcgtcgg tcatgggcac
      961 gagcaccggg atgccaccct gcgtgcgcgt gcgggcctcg tacatgctga acgtcggaac
     1021 gctgagcagc acttcctcgc ccgggcccac gaacgtgcga atcacgacgt cgatgagctc
     1081 ggtcgaaccg gctccgagca gcacgctgcc cgggctcaac gcctcgcgcg ccgcgagcct
     1141 ttgcgcgagt tggtcggcac cgctcgggta gaggttgccg cgcaacgccg cctcgacgat
     1201 cgcgttcacg acccgagcgc tcggggcgta cgggctctcg ttcgacatca tgcgcgtgat
     1261 gccggcctga ctctcggcga ccgcgaagtg cgactcgttg tacggctttg ccgcgcgcac
     1321 ccagctggga gcaaacattt cgagcgcttc gggatcgatc gaaacgggtg cccccgaacc
     1381 gggctcgtcg cccaggctgt cgccgaagac gcgcagcatc gcctctgcgc gctcgagcgc
     1441 gggaggcgta tcgacgtcga tccagcgcac ctcgcccacg tcgacggcgt agaaatcacc
     1501 gcgccgcgcg agggcgtaga cgccgtccga cagcgagcag tctccgcgct cttggtcgag
     1561 cttgcggagc tcgtcgatga gcgcgggacc aatgcggaag acgccggtgt cgagcgcgtc
     1621 gtactcttcg agctctttgc cgatgcgcgc gatgtgcgtt cggtcgacga ccaccttggt
     1681 cgcgtcgtcg atgtcgaagc aacgttcgat gtcgtagtcg actccgagcg cgcacgcgcc
     1741 ggcgggcaag tcccacgcga tgagacgacg cacgagctcg ggcgaataca gatgatccga
     1801 catcgtcagg atgcactcct ggtcgatgtg ctcggccgca gtgagcagcg atacgccgtt
     1861 cttcttgtcg aagcgcgggt tctcgacgaa cgtgagcttc aacccgaggc tcggctcgga
     1921 gagcaaggcc ttgcgaacac gctcgccctg gtagccgatg atgactaccg cctcgcggat
     1981 gcccgcttgt tcgagggtgc ggagcaccct aacgcacaac ggcacacccc caaccggccg
     2041 gagcggcttc ggcagctcgt tctcgtcacc ctctcggagg cgggagccgg ttccggctgc
     2101 caggatgact gctcgcgtca cgcgtcggtc ggtcatgatc aggactcgct ttcgtcaatc
     2161 gggcgctaag ttcgtagggc attagcacgc gccgtgacaa cccggcaaat ccttttccaa
     2221 tcaagtactt agaccccaat ttcccctgtg agaacggcac cacactgtag caattttgcg
     2281 acaaaggcgt tctcgaaata ctgcagtgcg gcagcacgat ttgaagcaga aaagctacca
     2341 agtagccact tttcacacat gggccacagc agcccgcccg tccttgtgta ctgcgtcgca
     2401 atttacgcag ctcaccggaa cattaccgct taactgcacc gcgcgctcgc tcgaggtctt
     2461 cgaaggtgtc gacctccgac cacggaagat cgccaacctc gaccgcttcc gcggagatgg
     2521 cgccttcggc gaccagctcc gaatagaaat gctcgtagta ctggtcgcga gaaccagcag
     2581 ccatcgcgct tccgatgcga gcgagcacgc gatcgccgcc gacgttcgtg agccgctcga
     2641 tgccgatcgt ctctgccttc gcgctcgcga gcggaatctc cttgccgaac gcactgatga
     2701 cgctcccttg cgcccgcacc ttcattgcct ccgcgtcgag tgcccgctgt gaatcgacgg
     2761 caacggcgag atctgctcga ctcaccgaga gtcgtgtcag cacgtccggt tgaaagacga
     2821 cgtcaccgtc gagcttgaag aagctcttgc cacgcaacgc gctccgacaa agccccagcg
     2881 aaacgctgtt ttgcgtgctg tcgtaggcgt cgtttctgaa gtagctgatt ttcgcgtcga
     2941 ggaactgcag cgcggcgcgg atttggtctt cgcggtagcc agtcgcgagc acgacttcgg
     3001 agactccgtg cgcgattaga aggcgaatcg cgcggttcaa gatggtctca ccgccgatat
     3061 ccatcagcgc cttcggccgg tcgttggtga gtggtcgcaa tcgcgaacca acgcctgcga
     3121 ccaaaatcac ggcagcgtcg gcgcggggag atgtggagtt gctcatcggc acggcgcttg
     3181 ctaccacgcc gcgcgtcatg caacgacctc aaaagctgga atcacctcag ggcaagctcg
     3241 ccgtgctgct gccgggcatg ggcgccgtcg ccacgacctt catcgcaggc tcactgctag
     3301 cgcgccgcgg cttggcgcgg ccgatcggcg ccatcacgca gaccggcacg attcggctgg
     3361 gcaagcgcac cgacaatcgc aacccgaaga ttgccgactt cgtccccctc gcgaatctca
     3421 acgacctcgt cttcggcggt tgggacctct tccccgacaa cgcctacgaa gccgcgacga
     3481 aagccgaagt gctcgagtcg aagcacctcg acccgatccg cgacgagctc tccgcgatca
     3541 agccgatgag cgccgtgttt tacccggagt acgtgaagcg cctcacgggc agcaacgtga
     3601 agaccggcaa gacgaaggcg gacatggtcg aggccgtgcg cgacgacgtg cgccgcttca
     3661 tcaagcagaa cggctgctct cgcgccgtcg cggtgtggtg cggctcgacc gagatctacc
     3721 tcgagccgcg ccccgtgcac caatcggtcg cggcgttcga gaagggtttg ctcgagaacg
     3781 atccttcgat ttcaaactcc atgatctacg cctgggcctt gctgaaggaa ggcattccgt
     3841 acgcgaacgg cgcgcccaac ctcaccgtcg acatcccggc gatgcacgag ctcgcccgcg
     3901 agaccagcag cccgacttgc ggcaaggatt tcaagaccgg ccaaacgttg atgaagacca
     3961 tcctcgcgcc ggggttcaag gcgcgcatgc tcggcatgaa gggctggttc agcaccaaca
     4021 tcctcggtaa ccgcgacggc gaggtgctcg acgatccgga caacttcaag tccaaggagg
     4081 tcagcaagct cggcgtgctc gaagacgtgc tgcagccgca cgtttacccc gagctctacg
     4141 gcgacctcta ccacaaggtc cgcatcaact attacccccc gcgcggcgac gcgaaggagg
     4201 gctgggacaa catcgacatc ttcggttggc tcggctaccc gatgcaaatc aaggtcgact
     4261 tcttgtgccg cgattcgatc ttggcggcgc cgctcgtgct cgacctcgcc ctcttcatgg
     4321 atctcgcgca gcgcgccgga ttccgcggtg tgcaagagtg gttgagcttc tacttcaaga
     4381 gcccgatgac ggcgcccggc ctctacccgg agcacgacct cttcatccag tcgatgaagc
     4441 tgaagaacac gctccgctgg atgatgggcg aggagctcat cacgcacttg ggccacgagt
     4501 actacgactg agcagaagtc gtcttcggac tccgagcgcc gagggccgtc gtggtcctcg
     4561 gcgttcgtgt tttcaggaga gccccgagcg gcgacgtgcg atccagtgcg cgcccaacgc
     4621 gagcgcggcg ccgagcgccc aaacccacgc cggcagcaac ggcgagacct cgcgctcgac
     4681 cgagac
//
LOCUS       DASTKS010000521         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_528657, whole genome shotgun sequence.
ACCESSION   DASTKS010000521 DASTKS010000000
VERSION     DASTKS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528657"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VFQ35_10815"
     CDS             complement(<1..1321)
                     /locus_tag="VFQ35_10815"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9931172.1"
                     /translation="MTSSRTSGTRPGSESPNAESPNRGAPVVAVTPSLAELLGDGSEF
                     GRTRTKASGPSGQLPLTREMLLREPSGNLFGMTQDAGMGWNPAKVDSPQYLILSTQGG
                     LRGPTGEPIALGYHTGHWEIGLLVKAAAEALREADVMPFAAYCSDPCDGRTQGTNGMF
                     DSLPYRNDAAITMRRLIRSLPRRVGVMGIATCDKGLPAMLLALAGSGHLPGILVPGGV
                     TLPADGAEDAGKVQTIGARFAHGLIDLEHAASMGCRACGTPGGGCQFLGTAATSQVVA
                     EAFGLSLPHSALSPSGEAVWLELARRSASALMGLVHAQIPMSRILTPPALENAMLVHA
                     AFGGSTNLLLHIPAIAHAGGLTRPTIEDWKRVNRATPRLVDALPNGPENHPTVRVFMA
                     GGVPEVMLRLRDAGLLHLDVLTVTGQKLGDVLDAWKESPRRHRSRERL"
     gene            complement(1318..2919)
                     /locus_tag="VFQ35_10820"
     CDS             complement(1318..2919)
                     /locus_tag="VFQ35_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9931173.1"
                     /translation="MSTTQLAQVLIDGRFRSATNPLGSFHAFDPTTRRELEPEFPISG
                     REDVAAALKAAVRAVDELEQLSPEARAHFLELYAAELEKNAEQLVAAAARETALPAAT
                     RLRQVELPRTVNQLRTAAAAVRKRDWCLATIDTRSGLRARFGALGGAVLVLGPNNFPF
                     AFNAVSGGDFAAAIAAGNPVIAKAHPSHPETTRLLADRALAALTDSRLPRATLQLLYH
                     VPEELGLELVTRAEVGATAFTGSKRAGLKLKAAADRAGKPIYLEMSSSNPVFFLPGAL
                     RERGQKLADELYESCTLGAGQFCTRPGLSVIVDGPEAKAFLDALESRFSSGTAGTLLS
                     RSVESGLEANVAELTAAGARLVTGGKPVPDAGYCFANTLLRVNAREFLERAEALQADC
                     FGGVHLVVSARDFAELLAVARALLGNLTGSIYSDTVGSDDAAYLELASIVRRRVGRLL
                     NDKMPTGVAVSSAMNHGGPFPATGHPGFTAVGMPASLLRFAALHSYDNVRPARLPPEL
                     ADKNPTGKMWRYVDGEWTQRDLGVA"
     gene            complement(2931..>3246)
                     /locus_tag="VFQ35_10825"
     CDS             complement(2931..>3246)
                     /locus_tag="VFQ35_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9931174.1"
                     /translation="KVYDGSCALGPGIVLSDVEPLRDLPIQIVIERNSQRVFEGETRS
                     SRIKRGLEELAGYLFRELSFPAGAYLLTGTGIVPPAEFSMQRGDVVRIRIAELELENP
                     VS"
BASE COUNT          563 a         1031 c         1119 g          533 t
ORIGIN      
        1 ggagccgttc gcgagagcgg tgacggcgcg ggctctcctt ccaggcgtcg aggacgtcac
       61 cgagcttctg gcccgtgacg gtgaggacgt cgagatggag gaggccagcg tcgcgcaggc
      121 gaagcatgac ttcgggcacg ccgccagcca tgaagactcg caccgtggga tgattctcgg
      181 gaccgtttgg tagcgcatcg acgaggcgcg gcgtggcgcg gttcactcgc ttccagtctt
      241 cgatggtcgg gcgggtgaga cccccggcgt gcgcaatcgc gggaatgtgg agcaacaggt
      301 tggtcgagcc gccgaacgcc gcgtgaacga gcatggcgtt ttcgagcgcc ggcggcgtga
      361 ggatgcgcga catcgggatt tgcgcgtgaa cgaggcccat gagcgcgctg gccgagcgtc
      421 gcgcgagctc gagccacacc gcctcacccg atgggctgag cgcgctgtgc ggcaggctca
      481 ggccgaacgc ttccgcgacg acttgtgacg tcgccgcggt tccgagaaat tggcatccac
      541 cgccgggagt tccgcacgcg cgacagccca tgctcgcggc gtgctccaag tcgatgagcc
      601 catgagcgaa gcgcgcgccg atggtttgaa ccttgcccgc gtcttcggcc ccgtcagccg
      661 gcaacgtgac accaccgggc actaagatgc cgggaaggtg acccgaacca gcgagcgcaa
      721 ggagcatggc gggtagccct ttgtcgcacg tggcaatgcc catgacgccg acgcgccgcg
      781 ggagcgaacg gatcaagcga cgcatcgtga tcgccgcgtc gttgcggtac ggcaagctgt
      841 cgaacatacc gttcgtgcct tgcgtgcgac cgtcacaggg atcgctgcag taggcagcga
      901 agggcatgac gtccgcttcg cgcagcgctt cggccgcagc cttgacgagc aagccaattt
      961 cccagtgtcc ggtgtgatag ccgagtgcaa tgggctcgcc ggtggggccg cgcagcccgc
     1021 cttgggtgct caagatcagg tactgtggac tgtctacttt ggcggggttc caacccatcc
     1081 ccgcgtcttg agtcatgccg aacaagttcc cgctcggctc gcgcaggagc atttcgcgcg
     1141 tgagcggcaa ctggccggat gggccactcg ctttggtgcg cgtcctgccg aactcggagc
     1201 cgtcgcccaa gagctccgcc aagctcggcg tgaccgcgac gacaggcgcg ccgcggttcg
     1261 gcgattctgc gttcggcgac tccgagccgg gccgcgttcc cgaggttcga gaggaggtca
     1321 agcgacgcct aggtcgcgtt gggtccactc gccgtcgacg taacgccaca tctttccggt
     1381 ggggttcttg tcggcgagtt ctggcggcaa ccgagccggt cgcacgttgt cgtaagagtg
     1441 cagcgccgca aaccggagca gcgaggcggg catgccaacg gcggtaaacc cgggatggcc
     1501 ggtggccggg aacggcccac catgattcat cgccgaagag acggcgacgc ctgttggcat
     1561 cttgtcgttg agcaagcgcc ccacccggcg acggacaatc gacgcgagct cgaggtacgc
     1621 cgcgtcgtcg ctgccgaccg tatcgctata aatgctgccg gtgaggttcc cgagcagcgc
     1681 gcgcgcgacg gcgagcagct ctgcaaaatc tctcgcgctg acgacgaggt gaaccccacc
     1741 aaaacaatcc gcttggagag cttccgcccg ctcgagaaac tctcgggcat tcactcgcag
     1801 cagcgtgttc gcgaagcagt agcccgcgtc cggaaccggc ttgccacccg taacgaggcg
     1861 tgcgccagcg gccgtgagct cggccacgtt cgcttctagg cccgactcga ccgagcgact
     1921 cagcagtgtg ccagcggtac ccgaactgaa gcgagactcg agcgcatcga ggaacgcttt
     1981 tgcttcggga ccgtcgacga tgacgctcag cccggggcgc gtgcaaaact ggccggcgcc
     2041 caacgtacag ctctcgtaga gctcgtcggc gagcttctgc ccgcgctcgc ggagcgctcc
     2101 cggcaagaag aagacggggt tcgagctcga catttcgagg tagatgggct tgccggcgcg
     2161 atcggccgcc gccttgagct tgagacccgc gcgcttgctc ccggtgaatg cggtcgcgcc
     2221 gacttctgcc cgcgtgacca actcgagccc gagctcctct ggcacgtggt aaaggagctg
     2281 caaagtggcg cgcggcaaac gcgagtccgt gagcgctgcg agcgcgcgat ccgcgaggag
     2341 gcgcgtggtc tccgggtgcg aaggatgcgc ctttgcgatg acgggattgc cggcggcgat
     2401 ggcggccgcg aaatctccac cgctcaccgc gttgaacgcg aacgggaagt tgttgggacc
     2461 gagcacgagc acggctccgc cgagcgcccc gaaacgagcc cgcaatccgc tcctcgtgtc
     2521 gatggtggca aggcaccagt cgcgttttcg aaccgcggcc gcagccgtgc gaagctgatt
     2581 tacggtgcgc ggaagctcga cctggcgcaa gcgggtcgcg gcgggcagtg cggtctcgcg
     2641 cgccgctgcc gcgacgagct gttcggcatt cttctcgagc tcggccgcgt agagttcgag
     2701 aaagtgagcg cgagcctcgg ggctcaactg ttcgagctcg tcgacggctc gcaccgccgc
     2761 cttgagagcg gccgcaacgt cttcgcgccc cgaaatcgga aattcgggct cgagctcgcg
     2821 gcgcgtcgtg ggatcgaacg cgtgaaacga gccgagcgga ttcgtcgcgg aacggaagcg
     2881 cccgtcgatg aggacttgtg cgagttgtgt ggtcgacatg ttcatcacgt tcacgagact
     2941 ggattctcta gttcgagctc cgcgatgcga atgcgcacga cgtcgccgcg ttgcatcgaa
     3001 aactccgctg gcgggacgat cccagtgccc gtcaacaggt aggcgcccgc tgggaaactg
     3061 agctcccgaa agagatagcc ggcgagctcc tcgaggcctc gcttgatgcg cgaggagcgt
     3121 gtttcgcctt cgaaaacgcg ttgcgagttc ctctcgatca cgatttgaat gggcaaatcg
     3181 cgaagcggtt cgacgtcgct gaggacgatg ccggggccga gcgcgcaaga gccgtcgtag
     3241 accttc
//
LOCUS       DASTKS010000522         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1643902, whole genome shotgun sequence.
ACCESSION   DASTKS010000522 DASTKS010000000
VERSION     DASTKS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643902"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..1626)
                     /locus_tag="VFQ35_10830"
     CDS             complement(343..1626)
                     /locus_tag="VFQ35_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET9931175.1"
                     /translation="MPVVSPLARELVFKIVYYGPGLGGKTTTLQYIHATAKPEHRGKM
                     VSLATPVDRTLYFDFLPIRLPSVRGMSVRLQLFTVPGQVYYNATRKLVLTGADAVVVV
                     YDSQRARMDANLESLENLRENLRAHGKSLEEFPHVVQYNKRDLYDVLPIEELDRQLNR
                     AGAPSLPTTATSGEGVYEALEAITRLSLDDFARRVPESRALDAGAHLELPEGGLVEAL
                     RLAEASPHAFANESTPAGPVEASADAPTPSMIRLSQLPDAEDPGEAAARAAAGDTPVP
                     PSPHTARAGQGLDEVGTKAESAPLASPGSRVPSMPALTFSFEALWPAAERPSVRELEA
                     ALAQGDFGRALTTADRLLARVLANTGALLGGSQDAPRDPATVALLLGLDGQRYLELRA
                     LVREVRSGRTATLNDALGAYATAIEARRLRSRVAR"
     gene            1868..>2114
                     /locus_tag="VFQ35_10835"
     CDS             1868..>2114
                     /locus_tag="VFQ35_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931176.1"
                     /translation="MTEQGALPLERERVLAARAQDGDRDALAELLRLHGPRLYRSVLL
                     PRLGSHAAAEDALSVTYMKVVERIAQFSWQEVGFYPWL"
BASE COUNT          328 a          692 c          727 g          367 t
ORIGIN      
        1 caccggtcca ggtctcgctg ccacttctcg ctgaggtcgc gcatcgcgct cgcctatgga
       61 gcacacggag cgccgaatgg aaagagaaaa gtggcggagt agtgctagaa cgccgatcag
      121 tttgactgct cggcgttcta gccttggttt gtgggggcgg cacgcgcagc ccccctcttc
      181 cgaaatcaat tcggaagaga ctcccacgcg gtcactccgt gcggagccgt tttccgctac
      241 ctccggcgaa tacctaaccg tttgtcgctc tagagagttt tccgaagtct ccccgctgct
      301 cgcatttctt ggctcgctac gggcaggaac ttgggacact ccctagcgcg cgacacgcga
      361 gcgcaggcgg cgcgcctcga tcgcggtcgc ataggcgccc aacgcgtcgt tcaacgtcgc
      421 cgtgcggccc gagcgcactt ctcgcaccag cgcgcgaagc tcgaggtagc gctggccgtc
      481 gaggccaagg agcaaggcga ccgtcgccgg gtcacgcggc gcatcttgcg atccgccgag
      541 gagcgccccg gtgttggcga gcacgcgcgc gagcaagcgg tctgccgtgg tcagcgcgcg
      601 accgaagtct ccttgcgcga gcgctgcctc gagctcacgc acgctgggtc gctcggccgc
      661 gggccagagc gcctcgaagc tgaaggtgag cgcgggcatg ctcggcaccc gcgagcccgg
      721 cgatgccagt ggagcggact cggctttggt cccgacctcg tcgaggcctt gcccagcgcg
      781 cgcagtgtgc gggctcggcg gtacgggggt gtcaccggcc gcggcgcgag ccgctgcctc
      841 acctggatcc tcggcgtcgg gaagttggga cagccggatc atcgagggcg tcggtgcgtc
      901 ggcggacgcc tcgaccggcc ccgctggcgt cgactcgttg gcgaacgcgt gcgggctcgc
      961 ctcggcgaga cgcagcgcct cgacgaggcc gccctcgggc aactcgaggt gagcccctgc
     1021 atcgagcgca cgcgactccg gcacgcgccg cgcgaagtcg tcgagcgaca gtctcgtgat
     1081 cgcctcgagc gcttcgtaga cgccttcgcc ggacgtagcc gtcgtcggga gtgacggcgc
     1141 gcccgcgcga ttgagttgac gatcgagctc ttcgatcggc agaacatcgt agaggtcgcg
     1201 cttgttgtat tgcacgacgt gcggaaattc ctcgaggctc ttgccgtgtg cccgcaagtt
     1261 ctcgcgcaag ttctcgaggc tctcgagatt tgcgtccatc cgcgcgcgct gcgagtcgta
     1321 aacgacgacg accgcgtcgg cgccggtgag aacgagcttg cgagtcgcgt tgtagtacac
     1381 ctgccccggc accgtgaaga gctgcaggcg cacgctcatg ccccgcaccg aaggcaagcg
     1441 aatcggcaag aagtcgaagt aaagagtgcg atcgaccggc gtcgcgaggc tcaccatctt
     1501 gccgcggtgc tcgggcttgg cggtcgcgtg gatgtactgc agagtggtcg ttttgccgcc
     1561 gagtcccggg ccgtagtaga cgattttgaa gacgagctcg cgagcgagcg ggctaacgac
     1621 gggcatcggg ggcctatgag catagccgtg ggacttcgcg gggcccagtc ctcgacctcg
     1681 gcgacgggct cgattccgag gggcgtaagg gaattgccgg ccgtgcgttc cccccatgac
     1741 cccgatgacg tttgccctgt gctccacgcc ccagagcctc ttcgttgggc gaggatccgc
     1801 gcgttggctg gcgtggtcgc tggaaatcga gctcggctgg ggggtaaatc gatctagagt
     1861 gcacgcggtg acggaacagg gagcgcttcc gctcgagcga gagcgagtcc tcgccgcgcg
     1921 agcccaggat ggcgaccgcg acgcgctcgc ggagctcctc cgcctgcatg gtccgcgttt
     1981 gtaccgaagc gtgctcttgc cgcgcctcgg cagtcacgcc gccgccgaag acgcgctctc
     2041 cgtcacttac atgaaggtgg tcgagcgcat cgctcagttc tcctggcaag aggtcggctt
     2101 ctatccttgg cttc
//
LOCUS       DASTKS010000523         6081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1422190, whole genome shotgun sequence.
ACCESSION   DASTKS010000523 DASTKS010000000
VERSION     DASTKS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422190"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1219)
                     /locus_tag="VFQ35_10840"
     CDS             complement(92..1219)
                     /locus_tag="VFQ35_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931177.1"
                     /translation="MSTRFILSNSSHVNSRSRLAPLLGSALIATLAAACSGSDATDVD
                     ATQSEQSLRAVIGAEGGELVGKAGTVFEGVRLSIPRGALVDKTEIEIVPATHLNPLPT
                     SAVGCGPEFEILPAGLKLDGEATLTLPFNEQTISDHYRFDDEVKVWAVMNGKWGQLLQ
                     TESSEGSVTVALQELNGVGAGINPPADSDIVKFDFAPNPKFAKCLARYPEDPSRAPHV
                     SAVVVRGELNDGLFLRGENVKPNLKFDMFSVERSSLLADATPDPNFKGFGLAWYQSDL
                     EANSYGRMNASIRTILLDQIFGFDPDVTLAPTGTFQMGFWFNNPDDAAECGFDVTKPT
                     PFNGEHRAGPLAMISLPNEETGLGPLCTKPDTSVSPARCDP"
     gene            complement(1431..2336)
                     /locus_tag="VFQ35_10845"
     CDS             complement(1431..2336)
                     /locus_tag="VFQ35_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931178.1"
                     /translation="MRNAQRCNSVLSSTHAGAALAFALVFAAGRARADVAMCVQSHAD
                     GQREAKAGHLKAAAELFATCGSTEGCPDTIRAECADLYRETQTNTPTAIFAALDANGN
                     DLTNVKVYAGDALLLDGLNGRAVALDPGKYTFKFELPSGQTLTNETLVREGEKNRIIS
                     VRVPKSPSPSPRLDEPLRHAPNRALPAPFWVSAGVGAAALASFGVFALLGRGKQSALD
                     DCAPRCPASRHDDFDAMRRDYLVADVSLGVGAVSLGLATWLFFSSQPSSATAATKSTH
                     QLAGVSVAPVISTHDAGLVATAVLF"
     gene            2606..4285
                     /locus_tag="VFQ35_10850"
     CDS             2606..4285
                     /locus_tag="VFQ35_10850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931179.1"
                     /translation="MSAAAIVERSHVRRAYRRLFELGRGGMARVYLAESLASGVRKLV
                     VLKALNPNLSADPEMREAFRREAELSAQMNHPNVVQVMEVVEHAQTPIIVMEYLDGVT
                     LSSLLKRARKDLPLALHLNILSQVLAGLHHFHELRDLEGAPLEAVHRDVSPQNIMVLH
                     EGGVKVLDFGIAKVSAPSDHATRTGIIKGKIQYMPPEQLLGRTKLDRRADVFAVGVML
                     WEAAAGRRLWKGSTEIELVRNLASGAVPSLREHAPHVPDPIVQVAERAMEIDCARRFA
                     TAKDMQAALDRALTDLGWFVRPRELADFMARHFGASRAEQEQKIKQALLQASESPPPA
                     DGLEAASLPALEIEPSAFGTVAPLGSPEARVESAPPTQGALDASATHLRRRNVRRSVA
                     TAALIACFALALLGLTRNRPSAAPVVSTRSATTTVSLQVDALPHGAQIYLDGALLGSD
                     HFAGSQPVRDHKAVLEVSAPGHLSERKEISLRKDIDLQIVLRPDPAANSETVKPDSTM
                     PAPDGSSASPRTRTRNGGRPSSKSSGNGSAKCTPPYTLGPDGIKTYKPECF"
     gene            4300..5256
                     /locus_tag="VFQ35_10855"
     CDS             4300..5256
                     /locus_tag="VFQ35_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9931180.1"
                     /translation="MFPASQALSRWALLCFAPLACSLQVPSEANVFGAESGGAPAGGN
                     EASGGVVTGGSANPNGGTTASSTGGTKANSSGGKAAGGAANGGSAESGAGALNAVDGL
                     IAYFPLDETSGTVAANVVNPNQSGSYFGSCTHPSGKRGMAVGIRNQPSNSDWVELPDG
                     LLSGLGEATVSIWVRDLSTARRGARLFDFSRGAGEGLYFSPDDDDPLASVAGAHLGGV
                     HSGTSFVDIWAPSPVLTDKIWHHVAVTWSGTNISLYIDGTTSGSKPRPTASPDDLGPT
                     TPNWLGKTLDDAFVALYAEIDEVRIYNRALEVREVGKLYQQP"
     gene            5588..>6081
                     /locus_tag="VFQ35_10860"
     CDS             5588..>6081
                     /locus_tag="VFQ35_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET9931181.1"
                     /translation="MKLVKPSQGLILEFKAVVAQLPRAEPRKMFGYDAAFVNGNMAVG
                     LWQDTCVLKLSAQHQAELLQAGHAKPFAPAKGRAMSAWLELSAELAHDPEELLVWSRR
                     AIEYTATLPPKVPKAAKVKTKATPSQGREPKPKGSRSKTASAKRSKTDAAKSPRRLRA
                     KASSA"
BASE COUNT         1086 a         2006 c         1925 g         1064 t
ORIGIN      
        1 gtaacctgac cggggcgaac aggcgcgctc gaaaagctct cgacatggcg cgcctcactt
       61 cgaccctatg cggctcgcgc ggttcgcgcc ttcacggatc gcagcgcgcc gggctgacgg
      121 acgtgtcggg cttcgtgcag agcggaccga gcccggtttc ttcgttgggc aggctgatca
      181 tcgcgagcgg accggcgcgg tgttcgccgt tgaaaggcgt gggcttggtc acgtcgaagc
      241 cgcactccgc ggcgtcgtcg gggttgttga accagaaacc catttggaaa gtgccggtgg
      301 gagcgagcgt cacgtccgga tcgaagccga agatctggtc gagcaagatg gtgcggatcg
      361 aggcattcat ccgcccgtag gagttggctt ccaagtcact ttggtaccag gcgaggccga
      421 agcctttgaa gttcggatcg ggtgtcgcat ccgccagcaa ggagcttcgc tcgacgctga
      481 acatgtcgaa cttcaagttc ggtttgacgt tctcaccgcg caggaagagc ccatcattca
      541 gctcccctct gacgaccacg gccgagacgt gcggcgctcg gctcggatct tctggatagc
      601 gagcgaggca ctttgcaaac ttcgggttcg gcgcaaagtc gaacttgacg atgtcggagt
      661 ctgcgggcgg gttgatccca gcgcccacac cgttcagctc ctgcagcgcc accgtgacgg
      721 aaccctcgct gctctccgtt tgcagcagct gtccccattt gccgttcatc actgcccaca
      781 ccttcacttc gtcgtcgaag cgatagtggt cggagatcgt ctgttcgttg aacggcaggg
      841 tgagggtcgc ttcaccgtct agtttcagcc ccgccggcaa gatctcgaac tcggggccac
      901 acccgactgc gctggtggga agcgggttca agtgcgtcgc gggaacgatt tcgatctcgg
      961 tcttgtcgac gagcgcgccc ctcggaatgc tgaggcgcac tccctcgaaa acggtgccgg
     1021 ccttgcccac cagctctccg ccctctgcgc caatcacggc gcgcaggctc tgctcggact
     1081 gagtcgcatc tacgtcggtg gcgtcgcttc cactgcacgc ggccgcgagc gtcgcgatta
     1141 gtgcgcttcc cagaagcggc gcgaggcgcg accgcgagtt cacgtggctc gagttcgaaa
     1201 ggatgaaacg agtgctcatg tctatctgct cctcagggtt cgggcgcgac gaggcgtcgt
     1261 cgtgccgacg tgaccccttc agcaacgccc atgccgaccc cgcgcgggcc cgttggaggc
     1321 gccgcggcac ggcgaatccg cgaatttgac gcgtttttgc cagcccaccc ttcgggccgg
     1381 acaccggcac gcgcgggcac gtgccgggca cgaacgcggg tgcggccgct ctaaaagaga
     1441 accgcggtgg cgactagtcc cgcgtcgtgc gtagaaatga ccggagccac cgagacgccc
     1501 gcgagttggt gcgtgctctt cgtcgccgcc gtggcgctcg acggttggct cgaaaagaag
     1561 agccacgttg cgaggcccaa ggagactgca ccgacgccga gcgagacatc cgctaccagg
     1621 tagtcgcgcc gcatggcatc gaagtcgtcg tgacggctcg cggggcaccg aggcgcacag
     1681 tcgtcgagcg ccgattgctt accgcgcccg agcagcgcga agacgccgaa tgaagcgagc
     1741 gcggccgcgc caaccccggc gctcacccaa aacggcgcgg gcagcgcacg gttcggggcg
     1801 tgacgtaaag gctcgtcgag tcgcggcgag gggctgggcg atttgggaac gcgcacggaa
     1861 atgatccggt tcttttcgcc ttcgcgaacc aatgtttcgt tcgtgagcgt ttgcccggaa
     1921 ggcagctcga acttgaaagt gtacttgcct ggatcgagcg ccaccgcgcg gccgtttagg
     1981 ccgtccagca ggagtgcgtc accggcatag actttcacgt tggtaagatc atttccattc
     2041 gcatcgagcg cagcgaaaat cgccgttggc gtgttcgtct gagtttcgcg ataaaggtct
     2101 gcgcactcgg cgcgaatcgt gtcggggcat ccctcggtgg agccgcacgt cgcgaagagc
     2161 tccgcggccg ccttcaggtg gcctgctttc gcttctctct gaccgtcggc gtgcgactgg
     2221 acgcacatcg ccacgtcggc gcgtgctctt ccggcggcga aaaccaaggc gaatgcgagc
     2281 gctgcgcctg catgagtcga agacagaacg ctgttgcatc gctgcgcatt tctcatggcc
     2341 aatttgtttc gtcgactccc gcggctcgac gcggacggtg agcatacgcg atcgctcgca
     2401 acctgggcac gtggaaccga gttcagtaac cgcttcgttc ggcatccgtc agcccaacgt
     2461 aacgtgcttg cgaaacgagc ggggccgtcg tcacccgcgt ggtgtgcatc tcgcacacat
     2521 gaagcagcat gtgcgaccag tgtcgccccc agctcactcg ggcggcccaa ccgcttgcgg
     2581 ctctgtgtta cttttggcct ggttcatgag tgcggcggct atcgtcgagc gtagtcacgt
     2641 gcgtcgcgcc tatcgtcggc tcttcgagct cggacgcgga ggcatggcgc gcgtgtacct
     2701 ggcggagagc ctcgcgtccg gcgttcgcaa gctcgtcgtg ctcaaggcgc tgaatccgaa
     2761 cctcagcgcc gaccccgaaa tgcgcgaagc ctttcgtcgg gaggccgagc tttcagcgca
     2821 gatgaaccat ccgaacgtcg tccaggtgat ggaggtcgtc gagcacgcgc agacacccat
     2881 catcgtgatg gaatacctgg atggggtcac actctcgtcg ctcttgaagc gcgcgcgcaa
     2941 agaccttccc ctggcgctcc acctgaacat cctctcgcag gtgctcgcgg gccttcacca
     3001 ttttcacgag cttcgcgatc tcgaaggcgc gccgctcgaa gcggtgcacc gcgatgtttc
     3061 gccgcaaaac atcatggtgt tgcacgaagg cggcgtgaag gtcttggact tcggcatcgc
     3121 caaggtgagc gccccgagcg accacgcaac gcgcaccggc atcatcaagg gcaagatcca
     3181 atacatgccg cccgaacagc tcctcggtcg gacgaagctc gaccgacgcg cggatgtctt
     3241 cgcggtgggc gtgatgctgt gggaagccgc cgcgggccga cgcctctgga aggggtcgac
     3301 cgagatcgag ttggtgcgga acctcgcatc tggcgcggtg ccgtcgttgc gcgaacacgc
     3361 gccgcacgtc ccagatccca tcgtgcaagt cgccgaacgc gcgatggaga tcgactgcgc
     3421 tcgacgcttc gccacggcga aggacatgca agcggcgctc gatcgcgcgc tcaccgacct
     3481 cggttggttc gttcgcccgc gcgaactcgc cgacttcatg gctcggcact tcggggcaag
     3541 tcgcgccgag caggagcaga agatcaagca agctctgctt caggcgagcg aatcaccgcc
     3601 gccagcagac ggtctcgaag ccgccagctt gcccgctctc gaaatcgagc cgtccgcctt
     3661 cggaaccgtg gccccgctcg gttctccaga ggcgcgcgtc gaatccgcgc ctcctacgca
     3721 gggggccctg gatgcgagcg ccacgcacct gcgacggcgc aacgtgcgcc gttcggtcgc
     3781 tacggccgca ctcatcgcct gctttgctct agccctgctc gggctcactc gaaatcggcc
     3841 gagcgcggcg cccgtcgtga gcactcgttc cgcaacgacg accgtcagcc tgcaagtcga
     3901 cgctttgccg cacggcgcgc agatctacct ggacggagcg ctgctcggaa gcgatcactt
     3961 cgccgggtcg caaccagtgc gtgaccacaa ggccgtgctc gaagtcagcg cgccggggca
     4021 tctcagcgaa cgcaaagaga tcagcctacg aaaagacatc gacctgcaga tcgtgcttcg
     4081 gcccgatccg gccgccaaca gcgaaacggt gaagccagac tccaccatgc ctgcaccgga
     4141 tggcagttcc gcgagcccgc gaacgcggac gcgcaacggc gggcgaccat cctcgaagtc
     4201 gtcggggaat gggtccgcca agtgcacgcc cccgtacacg ttggggcccg acggcattaa
     4261 aacctacaag cccgagtgtt tttgactgtc ttcgcccgca tgttccctgc gtcgcaagcc
     4321 ttgtcgcgtt gggctctact ctgcttcgct cccctcgcgt gctctctgca agtgccgagc
     4381 gaagcgaacg tattcggcgc ggaaagcgga ggcgctccgg cgggcggcaa cgaggcgagc
     4441 ggcggagtcg ttaccggcgg cagcgcgaac cctaacggtg gcaccaccgc gagttccacc
     4501 ggcggcacca aagccaactc gagcggcgga aaggctgcgg gaggcgccgc gaacgggggc
     4561 tcggcggaga gcggcgcggg cgcactcaac gcggtcgatg gcctcatcgc gtacttccca
     4621 ctcgacgaaa cgagcggcac cgtcgccgcg aacgtcgtga accccaatca gagcggcagc
     4681 tacttcggtt cctgcacgca tccgtcgggc aagcgcggca tggcggttgg aattcgcaac
     4741 caaccgagca actccgattg ggtcgagtta cccgatggcc tgctgagtgg gctcggcgaa
     4801 gcgaccgttt cgatttgggt tcgcgatctc tccaccgctc gaaggggcgc gcgtctattc
     4861 gacttcagtc ggggtgccgg cgaagggctc tacttttccc ccgacgatga cgaccccttg
     4921 gcctccgtag cgggcgcaca tctcggcgga gttcattcgg ggacgtcctt cgtcgacatt
     4981 tgggcgccct ctccggtatt gaccgacaag atctggcacc acgtcgcggt cacttggagc
     5041 ggcacgaaca tcagcctgta catcgacggg acgacttccg gcagtaagcc gaggcccacc
     5101 gcgtcgcccg acgatctcgg ccccacgacc cccaattggc tcgggaagac tctcgacgac
     5161 gcgttcgtcg ccctctacgc cgagatcgac gaagttcgca tttacaaccg tgcgctcgag
     5221 gtcagagagg tcggtaagct ctatcagcag ccataggttt ggagggcccg ccacgtcgac
     5281 gatgctcgcg gctctcgctc cgcccaccgg cgcagccgcc gtcgcttgat cgagggccgg
     5341 cgccgtcgaa cgacgccggc cctcgatttt gcagccccgt tcgcccgcta ctcacaccca
     5401 gagtgttccg cccctgctac gccgatcagt gcctcttcgc gccccgatcc cgcgccgaag
     5461 aacctcctcg agaggaatga tccgtccatc ctcgtgcaat gtccgaggcc gcggattttg
     5521 atccctaccc gtcttgcgcg gacggatgtg ttggcgaggc cacggtcaac ggttacgttg
     5581 agtcaccatg aagctcgtca aaccaagcca gggcttgatt ctagagttca aagcggtcgt
     5641 agctcagctc ccgcgcgccg aaccgcgaaa gatgtttggt tacgacgcgg cgttcgtgaa
     5701 cggcaacatg gcggttgggt tatggcagga cacttgcgtg ctcaaactgt cggcacagca
     5761 tcaggccgag cttctccagg cggggcatgc caagcccttt gctcccgcta aaggtcgcgc
     5821 gatgagcgcc tggctcgaac tctccgcgga gctcgcccac gacccggaag agctactcgt
     5881 ctggagtcga cgcgcgatcg aatacaccgc taccctgccc ccgaaggttc cgaaggccgc
     5941 aaaggtcaag acgaaagcca cgcctagcca ggggcgggaa ccaaaaccca aaggttcgcg
     6001 gtcgaagact gcctcagcga agcgctcgaa gaccgacgcc gccaagtcgc ctcgccgact
     6061 ccgagcgaaa gcatcgagcg c
//
LOCUS       DASTKS010000524         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_750902, whole genome shotgun sequence.
ACCESSION   DASTKS010000524 DASTKS010000000
VERSION     DASTKS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750902"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFQ35_10865"
     CDS             complement(<1..375)
                     /locus_tag="VFQ35_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931182.1"
                     /translation="MPLGKRQRLLIPCFLLSQLSCSGASREAMSPSARLEVGADDALK
                     TPSTGTPYAFHNVAIRGGGFVTGIVFSEAKAGIVYARTDVGGAYRFDPTRNTWLPLMD
                     SFGRADSNLTGVESIAADPHDAD"
     gene            complement(515..>2021)
                     /locus_tag="VFQ35_10870"
     CDS             complement(515..>2021)
                     /locus_tag="VFQ35_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9931183.1"
                     /translation="GAVLVFESANPTREHRLAVKQKVSALRFHPNAERLVVAGELGMV
                     SIWDFAGTKELELRGHNGTVRALAFAQDGHFLASGGEDHSIRFWDSTTGETGAAPILH
                     SDAITSLSWAEDGKVLAYGSKDKSLHLVELKAAEDKPTLVRYHDDSVDAVALAPDLSE
                     LVSMSRDLGAVTWSLDGIRKGSPLVERGNVLSLAVVPGRPEVISGGLGVNGVGIWNVA
                     TGEADTRLPAGVDRIRSLAASPDGQKLAFAGSAGRVFLWDLPSRIPLRVFESGRDEVR
                     SVAFSGDNRWLAFGGLDRTLHVVGAQKFEDVARFTNSSPVQGIAFVPHSATIAAVDRD
                     GQVSGYDVAHLRQSFRFKAHDEWALAVAPAPDGKWLVTGGGDRHVKIWEAGGARLLAD
                     CRGHEGKVLSVDVSPDGRIVASGSEDKSVRLWDPTTGAELALLAGHSGAVRSVKFAPS
                     GDILMSGSDDGTIRLWRLAVLFTRAATLRQSVATRFHVELVGTRVVRSNEP"
BASE COUNT          346 a          695 c          649 g          331 t
ORIGIN      
        1 gtccgcgtcg tgcggatcgg cggcgatgct ttcgacgccg gtcaggttcg aatcggcgcg
       61 accgaacgaa tccatcagtg gcagccacgt gttgcgcgtc gggtcgaagc ggtaagcgcc
      121 gcctacgtcg gtgcgtgcgt agacgatgcc ggccttcgct tcgctgaaga cgattccggt
      181 gacgaacccg ccgccgcgaa tcgctacgtt gtgaaaagcg tacggcgtgc ccgtgctcgg
      241 cgtcttcagg gcgtcgtcgg cgccaacttc gagccgcgcg ctcggcgaca tggcctctcg
      301 cgacgcgccg ctgcagctca gttgtgaaag cagaaagcag gggataagga gtcgctggcg
      361 cttgccgagc ggcatgcctc ctattcgcac ggaagctgtc cgaggttcaa gccgggcgcg
      421 cctgtatgag gcgagggtga cacctgcgat tcacgggttc ggacccaagc ccgcgccgcg
      481 tgccgacgga gctgggtcgc aagagccgcg ccccctaggg ttcgttagag cgcacgactc
      541 gggtaccgac gagctccacg tggaagcgcg tcgcgacgct ctgccgcagc gtggcggcgc
      601 gggtgaagag cactgccaaa cgccacaatc ggatggtccc gtcgtcgctt cccgacatca
      661 ggatgtcacc cgaaggcgca aactttacgc ttcgcacggc gccgctgtgc cccgcgagca
      721 gcgccagctc ggctcctgtc gtcgggtccc atagacgcac gcttttgtct tcgctgccgc
      781 tcgcgacgat gcgtccgtct ggggaaacat ccacgctcag cacctttccc tcgtggccgc
      841 ggcaatcagc cagcagtcgc gcgccccctg cctcccagat cttgacgtgg cggtctccac
      901 cacccgtgac gagccacttg ccgtccggag ccggcgccac cgcgagagcc cactcgtcgt
      961 gagccttgaa gcgaaacgac tgacgcaggt gcgcaacgtc gtagccgctc acctgtccat
     1021 cgcgatcgac ggcagcgatg gtcgccgagt ggggcacgaa cgcgatgcct tgcaccggcg
     1081 acgagttggt gaagcgcgcc acgtcctcga acttttgtgc accgacgacg tgcagagtac
     1141 ggtcgagccc gccaaaagcg agccagcgat tgtcgccgga aaacgccacc gatcgtactt
     1201 cgtcacggcc gctctcgaag acgcgcaacg gaatgcggga cggcaaatcc cacaaaaaga
     1261 cgcggcccgc cgagcccgca aacgcgagtt tctgaccatc gggcgaggcg gcgagcgagc
     1321 ggattcggtc cacgcccgcg ggcaggcgcg tatcggcttc acccgtagcg acgttccaga
     1381 tgccgacccc gttgactccg agcccgccgc tgatgacctc cggcctgccg ggtacgaccg
     1441 cgagcgagag cacgttcccc cgctcgacca gcggcgaacc cttccgaatg ccgtccagcg
     1501 accacgtgac cgcaccgaga tcgcggctca tcgaaacgag ctcggagaga tcgggcgcga
     1561 gcgctaccgc atcgacgctg tcgtcgtggt atcggacgag cgtcggcttg tcttccgccg
     1621 ccttcagttc gacgaggtgc agcgacttgt ctttgctccc gtaggctagg accttgccgt
     1681 cttcggccca gctgagcgac gtgatcgcgt cggagtgaag gatcggcgcc gccccggtct
     1741 cgcccgtcgt cgagtcccag aatcggatcg agtggtcctc gccgccgctc gccaagaagt
     1801 gtccgtcttg cgcgaaagcg agcgcgcgca cggtcccatt atgaccgcgc aattcgagct
     1861 ctttcgtacc ggcaaagtcc cagatggaca ccatcccgag ttcgcctgcg acgacgagcc
     1921 gctctgcgtt cgggtgaaag cgcagcgcgg acaccttctg cttcactgcg aggcgatgct
     1981 cccgcgtcgg gttcgcgctc tcgaacacga gcacggcgcc g
//
LOCUS       DASTKS010000525         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1197904, whole genome shotgun sequence.
ACCESSION   DASTKS010000525 DASTKS010000000
VERSION     DASTKS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197904"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..596)
                     /locus_tag="VFQ35_10875"
     CDS             complement(120..596)
                     /locus_tag="VFQ35_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931184.1"
                     /translation="MGKKKTEKSEPDDIKIEPFRQNLPVILTKEEVAERAQRSAHLIA
                     DHDFKEAQFKDEAASNKRVLARINVQIRELSNEVRDGKTYQDVACERVFNWTNGTVHD
                     RRTDTGEVISERAMSEAEKQKALPFERPAGDIDDEFGDPKKPDAENEDDDGETAAE"
     gene            complement(577..1545)
                     /locus_tag="VFQ35_10880"
     CDS             complement(577..1545)
                     /locus_tag="VFQ35_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931185.1"
                     /translation="MTASAHLLQLVRAHYSGNESAFTSAAMTLARAAKVASTREAILD
                     IVREGVSAQRQGRRANPSQPQQFRPQAVAQTNGMLEELPPVTFASLQLEPYLQLALDE
                     FVVELEYREELATRNLRARNRFLFHGPPGNGKTSSAAALANALDLKAYAVSLPRLIGS
                     HVGETGQNLGKIFENLTPNTVVVFDEIDAVGASRGEPDQAASKEYNSNVNTLLTLLDR
                     NRAGVIVATTNRPDVVDAALLRRFDEQVEFPGPSPQQLRALADKLADGYDIPRLHRDA
                     IGDCLNYDAVTKTVEREARRVVMREILAAEAAAETESEEEHGQEEN"
     gene            complement(1542..1892)
                     /locus_tag="VFQ35_10885"
     CDS             complement(1542..1892)
                     /locus_tag="VFQ35_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931186.1"
                     /translation="MRCAHGFETSVVACPVGCGGPKPARQRKLSEPQPREPDQRVTAI
                     VEEVAAELGGKPEAIVTPSAGWTRAGGLTFPVRARAEVVRRATERFGLTRYQVAKALG
                     VPLKDLSYAVRRCA"
     gene            complement(1889..2194)
                     /locus_tag="VFQ35_10890"
     CDS             complement(1889..2194)
                     /locus_tag="VFQ35_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931187.1"
                     /translation="MAEIIDLAPHRRARTRQAFAAIYGSDDARLVRIDAALADAEEQL
                     VLARHRARCAPEHERAWREADALALARGIVELERIRLRLAGPELALILGGAAREPRS"
     gene            complement(2336..>3209)
                     /locus_tag="VFQ35_10895"
     CDS             complement(2336..>3209)
                     /locus_tag="VFQ35_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9931188.1"
                     /translation="ATTHDPGPVKTWELYVDAHFEPFFGALHNFTTAQAELYMRERLG
                     KVLADTVRREHTALRAFAGWAHANGFLPEPMIVPHVPRRAIGKPHSKRRRAKAIELSE
                     EETRRLIALLPAWSTSKRVKRFPIRARFEVAYETGLRPELLNSLMAPLHYRKGQETIR
                     ITPDLDKGRWARDVPLSDAARRALDKVVPKSGLIFGRHDYREHLRAAAKAVLPPDRAE
                     RFNGAHLRSARVTHLLEQPNANLPGVQFLVGHRSTATTARYVRPSARAAREVLGILGA
                     PRNSGMGKSAKERT"
BASE COUNT          487 a         1069 c         1041 g          612 t
ORIGIN      
        1 tcgcacgagg tgatctcgcg caggcggtgc cgccaggatc gcatcggcgc ggttcagcgc
       61 gtcggcgatc cacgcagcgt caacgtgcgt gccgcgtcgg gggagcggaa gcgatcgcgt
      121 cattccgccg cggtttctcc gtcatcatcc tcgttctcgg catccggctt cttcgggtcg
      181 ccgaattcgt cgtcgatgtc tcccgcgggt cgttcgaatg gaagagcctt ttgcttctcc
      241 gcctcggaca tggcgcgctc cgaaatgacc tcgcccgtgt cggtgcggcg gtcatggaca
      301 gtgccattcg tccagttgaa tacacgctcg cacgccacat cctggtacgt cttgccgtcg
      361 cgaacctcgt ttgacagctc gcggatctgg acgttgatgc gcgcgagcac gcgcttgttt
      421 gaggccgcct cgtctttgaa ttgcgcttcc ttgaagtcgt gatcggcgat caggtgagca
      481 gaacgctgcg cgcgctcggc cacctcttcc ttcgtcaaaa ttaccggcaa attctgacga
      541 aacggctcga ttttgatgtc gtccggctcc gacttttcag ttttcttctt gcccatgttc
      601 ctcttcgctt tccgtttcag ccgccgcctc ggcggcaagg atctctcgca tcacaacgcg
      661 ccgagcttcg cgctcgaccg tcttcgtgac ggcgtcgtag ttcagacaat cgccaatcgc
      721 gtcgcgatgc agcctcggaa tgtcgtatcc gtcggctagt ttgtccgcga gggcgcgcag
      781 ttgctgcgga gacggaccgg ggaactccac ctgttcatcg aatcggcgga ggagcgccgc
      841 atccacgacg tcgggacggt tcgtcgttgc gacgatcacg cctgcgcgat tgcggtccag
      901 aagcgtcaat agcgtattga cgttcgagtt atattccttg gacgccgcct ggtccggctc
      961 tccgcgcgag gctccgaccg cgtcgatctc atcgaagacc acgaccgtgt tgggcgtgag
     1021 gttctcgaaa atcttgccaa ggttctgccc cgtttcgcca acgtgcgaac caataagacg
     1081 cggcagcgag accgcatagg ctttcagatc gagcgcattc gcgagtgctg cggcagaact
     1141 cgtcttgccg tttcctggcg ggccatgaaa caggaaccga ttgcgcgctc tgagattgcg
     1201 cgtcgctagc tcttcccgat actcgagctc gaccacgaac tcgtcgagcg cgagttggag
     1261 gtacggctcg agctgaagcg aagcgaaggt gaccggcggc aattcttcga gcatgccgtt
     1321 cgtttgcgcg acggcttgcg gccggaactg ctgcggctgg gacgggtttg cgcgccggcc
     1381 ctgccgctgc gcgctcacgc cctcgcgcac gatgtcgagg atggcctcgc gtgtgctcgc
     1441 caccttggct gcgcgcgcca gcgtcatggc ggcgctcgtg aaggccgact cgttgcccga
     1501 gtagtgggcg cgtacgagct ggagcaggtg cgctgacgcg gtcacgcaca cctcctcacc
     1561 gcgtacgaga gatccttcag cggaaccccg agcgccttgg ccacctgata gcgagtgagc
     1621 ccaaatcgct ccgtcgctcg gcgcaccacc tcagcgcgag cgcgaacggg gaacgtcagg
     1681 ccgccggctc gggtccaccc ggcggagggc gtgacgatcg cctcgggctt gccgccgagc
     1741 tcggcagcga cctcctccac gatagcggtg acgcgctggt cgggctcgcg gggctgcggc
     1801 tcggagagct tgcgttggcg ggctggcttc ggtcctccgc agccgactgg gcaggcgacg
     1861 acgctggtct cgaatccgtg agcgcacctc atgatcgagg ctcccgcgcg gcgccgccta
     1921 gaatcagcgc cagctccggc ccggcgagtc gcagccgaat ccgctcgagt tcgacgatgc
     1981 cgcgagcgag cgctaacgcg tcggcctctc gccacgcccg ctcatgctcg ggcgcgcatc
     2041 tggcacggtg gcgcgcgagc acgagttgct cctcggcgtc ggccagcgcg gcgtcgatgc
     2101 gcaccaggcg ggcatcgtcg gagccgtaga tcgcggcgaa tgcctgccgc gttcgggcgc
     2161 ggcggtgggg ggcgaggtcg atgatctcag ccacacggcc cccaggaaag cccccaggtc
     2221 tcacgacgct ccgagcattt gtctgctgtt ttctgagacg ctatccgcga cgaaccagca
     2281 tcgaaacgct tttcctgatt tattgaggtg ctagcggggt tactcccatg gaggttcaag
     2341 tcctctcctt cgcactcttt cccatccccg aattcctggg ggcccccagg atccccaaaa
     2401 cctcgcgcgc ggcgcgagca gaggggcgca cgtaccgtgc cgtcgtggcg gtcgagcggt
     2461 gcccaaccaa gaactgaacc ccgggcaggt tcgcgttggg ctgctcgagc agatgcgtaa
     2521 cccgggcgct ccgcaggtgg gcgccgttga accgctccgc tcggtccggc ggcagcaccg
     2581 ccttcgcggc tgctcgcagg tgctcccggt agtcgtgcct gccgaagatg agacccgact
     2641 tcggcaccac cttgtcgagc gcgcgccgcg ccgcgtccga aagaggcacg tcccgagccc
     2701 aacggccctt gtcgagatcc ggcgtgatcc ggatcgtctc ctgccccttc cggtagtgca
     2761 acggcgccat gagcgagttc aggagctcgg gccgaagccc cgtctcgtag gccacctcga
     2821 aacgggctcg aatcgggaaa cgcttgacgc gtttgctcgt gctccatgct ggcaagagcg
     2881 cgatgagccg gcgcgtctcc tcctcggaga gctcgatcgc cttcgcccgt cggcgcttgc
     2941 tgtgcggctt gccgatcgcg cgcctcggca cgtgtggaac gatcatcggc tcgggcagaa
     3001 aaccgttcgc gtgagcccag ccggcaaatg cccgcagggc cgtgtgttct cggcgcaccg
     3061 tgtctgcgag caccttgcca agtcgctccc gcatgtagag ctcggcctgg gcggtcgtga
     3121 agttgtggag cgcgccgaag aacggctcga agtgcgcgtc gacgtagagc tcccacgtct
     3181 tgaccggtcc cgggtcgtgc gtcgtcgcg
//
LOCUS       DASTKS010000526         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_974700, whole genome shotgun sequence.
ACCESSION   DASTKS010000526 DASTKS010000000
VERSION     DASTKS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974700"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..405)
                     /locus_tag="VFQ35_10900"
     CDS             complement(139..405)
                     /locus_tag="VFQ35_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931189.1"
                     /translation="MSLGVGPGSVPHQNEAQFLMANDPNADALGRNRCVDCGTLSPKT
                     DTNYTLISARHGWRLTRQVDANGRNVLEWRCPRCWEKYRKRDTV"
     gene            complement(441..1667)
                     /locus_tag="VFQ35_10905"
     CDS             complement(441..1667)
                     /locus_tag="VFQ35_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9931190.1"
                     /translation="MANSWPFPRVDTLPTYGFVVTDSVKARVLARGVDLIDLGLGNPD
                     RPTPKQIVAQLHEQADIGTNHRYHPGRGIPPLRDAAARWYQRRYGARFDVNREVVITM
                     GAKEGMSHLCLAVLDVGDVVIVPDPCYPIHAGAPRIAGAEVEYYQATPDIAPAAAVAA
                     ALARVKARGKRTKLVIANYPQNPTGQVVTKRELGDLVQVVGESGAMLLHDLAYADFDF
                     QSRFAPSVFDVGVDPELVKSFAVEVFSASKSYNMPGWRIAFMVGNERMISALAHLKTY
                     LDYGTFAPIQLAAAWALDNGQGAADELRALYKVRAEALVRGLRAAGFPDVRPPSGTMF
                     VWTRLPEPWLRLGSVGAASYLIEEAYVATSPGSGFGPGGEGFLRFALIEDPPRIEEAC
                     ARLGRLLEKDSRKGEG"
     gene            1860..2864
                     /locus_tag="VFQ35_10910"
     CDS             1860..2864
                     /locus_tag="VFQ35_10910"
                     /inference="COORDINATES: protein motif:HMM:NF016822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HET9931191.1"
                     /translation="MRSDEEELLALAGFLNTVNLPNDRAHIQRLLEHSERSFQGEIAK
                     NHRKFVFLLRDVAEERAVGTSMIVAQLGRRDAPYIYLDVISEEKYSHAVDKHFHHTVL
                     KIGFSYKGPTELGGLVVHPDHRRANERLGLLISYVRFLYIATHRELFQDEILAELLPP
                     LEPDGTSHLWEALGRHFTGMSYAEADRLSSTDKTFIRDLFPSGDIYASLLSADAQSVI
                     GKVGAQTRGVEKMLRRVGFRYAERIDPFDGGPHFVAGVDDVSLIQETRERRLCDLRAP
                     SAGSPRALVGRDDTKPPYFRAAACAVELGADDSLIVEEDVAARLGVKVGDRLVVLPLR
                     "
     gene            complement(2906..>3367)
                     /locus_tag="VFQ35_10915"
     CDS             complement(2906..>3367)
                     /locus_tag="VFQ35_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9931192.1"
                     /translation="VFQFHHLLPEFNALENVMLPGLIQGRPRKEMEKRARSLLEEVGL
                     GSRTTHRPGELSGGEQQRVAVARALALDPKLLLADEPTGNLDTATSDAVHDLFFQINR
                     EHGTTIVVVTHNPAFAERMPRVVNMRDGRVEHDDVRTGDKGVAPASPSLAV"
BASE COUNT          611 a         1086 c         1090 g          580 t
ORIGIN      
        1 agcgcagcac cgggaggcgc gggtacttgg ggaagcgcgg ctctacctcc gcccgacggc
       61 agagccaaaa ctcgctcccc ttagccgaac ggatcacgcg cgcgaagcgg caggaaacgc
      121 aaagtccggg cgacacgctc agaccgtgtc gcgctttcgg tacttttccc agcaacgcgg
      181 gcaccgccac tcgagcacgt tccgcccgtt ggcgtcgact tggcgcgtca aacgccagcc
      241 gtgtcgggcg ctgatgagcg tgtagttggt gtccgtcttc ggtgagagcg tgccgcagtc
      301 cacgcaacgg ttgcgcccga gggcgtccgc gttcgggtcg tttgccatga ggaactgcgc
      361 ttcgttttgg tggggcacac tacccgggcc aacaccaaga ctcatttctt acacagccca
      421 cttcaggccg caagccggcg ctacccctct cccttccggg aatccttctc caacagtcgt
      481 ccgagccgcg cacaagcctc ctcgatgcgg ggcggatcct cgatgagggc gaatcgaagg
      541 aacccctcgc ccccggggcc aaagccactc cccggtgagg tcgcgacgta cgcctcttct
      601 atcaggtagc ttgcggctcc caccgaaccc aggcgaagcc acggctccgg caagcgtgtc
      661 cagacgaaca tcgtcccgct cggcgggcga acgtcgggga accccgcggc gcggaggccg
      721 cgcaccaaag cctcggcccg gaccttgtag agggcgcgaa gctcgtcggc cgcaccctga
      781 ccgttgtcga gcgcccacgc cgccgcgagt tgaatgggcg cgaacgttcc gtaatctagg
      841 taggtcttca ggtgtgcgag cgcggagatc atgcgctcgt tgccgaccat gaaagcgatg
      901 cgccaaccgg gcatgttgta gctcttcgag gccgagaaca cttcgaccgc gaaggacttc
      961 acgagttcgg gatcgacgcc cacatcgaac acactcgggg cgaagcgcga ctggaagtcg
     1021 aagtcggcgt acgcgaggtc gtggaggagc atcgcgccgg actcgcctac gacttgtacg
     1081 agatcgccga gctcacgctt cgtcacgacc tgcccggtcg ggttctgggg atagtttgcg
     1141 atgaccagct tggtgcgttt gccacgcgct ttgacgcggg cgagcgccgc agcgaccgcc
     1201 gcggcgggcg cgatgtcggg agtcgcttgg tagtactcca cctccgcacc ggcgatacga
     1261 ggcgctcccg cgtgaatcgg atagcaagga tccggcacga tcacgacgtc gcccacatcg
     1321 aggacggcca agcacagatg gctcatgccc tccttggcgc ccatggtgat gaccacctcg
     1381 cgattcacgt cgaagcgcgc gccgtaacgg cgctggtacc aacgcgcggc cgcatctcgc
     1441 aacggcggga tcccgcgacc cgggtgatag cgatggttcg tgccgatgtc ggcctgctcg
     1501 tgcagctgcg cgacgatttg cttgggcgtc ggacgatccg ggttaccgag cccgaggtcg
     1561 atgaggtcga ctcctcgagc gagcacccgc gccttgacgg agtcggtgac gacgaatccg
     1621 taggtgggaa gcgtatcgac gcgcggaaaa ggccacgaat ttgccacggg ggcgctccct
     1681 taacacgaaa gggaacggaa acaatggcgt ggagcagctc ggtcaccgtg ccgcgcaggt
     1741 cgacgcgcac cggccccaca accaagccgc tttcacctgt agtttccgac ggtttcgccg
     1801 cgaaatcccg ccgtgttaca cagagaggag tcatgctgcg ctacgaagtt cgaggagcta
     1861 tgcgaagcga cgaggaggag ctgctcgcgc tcgcgggctt cctgaacacc gtcaatctgc
     1921 cgaacgatcg agcgcacatt cagcgcttgc tcgaacattc ggagcggagc ttccaaggcg
     1981 agatcgcgaa gaaccatcgg aagttcgtgt tcctgttgcg cgatgtcgcg gaggagcgcg
     2041 ccgtgggcac gtcgatgatc gtcgcgcagc tcgggcggcg tgacgccccg tacatctatt
     2101 tggatgtcat cagcgaagaa aagtactcgc acgccgtcga caagcacttc caccacacgg
     2161 tgctcaaaat cggattttcc tacaagggac caaccgagct cgggggattg gtggtgcacc
     2221 ccgatcatcg ccgcgcgaac gagcgcctcg gccttttgat ctcgtacgtg cgctttctgt
     2281 acatcgcgac gcaccgtgag ctcttccaag acgagatcct cgcggagctg ttaccgccgc
     2341 tcgaacccga cgggacgagt catctgtggg aggcgctcgg ccgccatttc accggaatga
     2401 gctacgcgga ggcagatcga ctctcgagca ccgacaagac gttcattcgc gaccttttcc
     2461 cgagtggtga catctacgcg agccttctga gtgcggacgc gcagagcgtc atcggtaaag
     2521 tcggcgcgca gacgcgaggc gtggagaaga tgctgcgccg cgtgggcttt cgttacgccg
     2581 agcgcatcga tcccttcgac ggcggaccgc atttcgtcgc cggtgtcgac gacgtgagcc
     2641 tcatccaaga gacgcgcgaa cggcgcctct gcgatctgcg cgcgccgagc gctggctcgc
     2701 ctcgagcgct cgtcgggcgt gacgacacga agccgcctta ctttcgtgcc gccgcgtgcg
     2761 cagtcgagct cggcgccgac gactctctca tcgtggaaga ggacgtggcg gcccggctcg
     2821 gcgtcaaagt cggcgaccgc ctagtcgtgc ttccgttgcg ctgagccaag ccccacgcgc
     2881 aaggccagta aactcgcgtg gctttctaga ccgcgaggga cggagatgcg ggggcaaccc
     2941 ctttatcgcc ggtgcgcacg tcgtcgtgct cgacgcgtcc atcgcgcatg ttcacgacgc
     3001 gcggcatccg ctcggcgaaa gccgggttgt gagtgacgac cacgatcgtc gtgccgtgct
     3061 ctcggttgat ttggaagaac agatcgtgca ctgcgtcgct cgtcgcggtg tcgaggtttc
     3121 cggtaggctc gtccgccagc aaaagcttcg gatcgagtgc caaggcgcgc gcaacggcga
     3181 cgcgctgctg ttcaccgccg gagagctcgc ctgggcggtg ggtggtgcgt gaaccgaggc
     3241 cgacctcttc gagcagagag cgcgctcgct tttccatctc tttgcgcggc ctgccctgga
     3301 tgagaccggg cagcatcacg ttctccagcg cattgaactc gggcaagaga tggtggaact
     3361 gaaacac
//
LOCUS       DASTKS010000527         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_529195, whole genome shotgun sequence.
ACCESSION   DASTKS010000527 DASTKS010000000
VERSION     DASTKS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529195"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VFQ35_10920"
     CDS             complement(<1..1069)
                     /locus_tag="VFQ35_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9931193.1"
                     /translation="MPNSSLSDSELFDRYLAQPATLPSSVCVAVETAFGEPEIVAFAM
                     ADLDRGLGLSQSWVVVGRERIAVVRGEPGAHRTECFRRNDVRGLSSEDGLSCQVLRLH
                     GETGSAPLVELYFTRRQRRAMGRIEFLLEQALEGRTVGTHDASAEYASSMLSQVREAQ
                     ALVTANKLSVVWRLLGYLRPYKRRLVWGMSAAFAITVFSMVPPLVTGFLVDRVIGPVQ
                     RAEVAATRVSSLAWLCIGGMVLVYVLRQLCLWVRLRLMAELGEFVARDLRDQVYSHLH
                     RLSLSFFGRKKTGSLITRTSSDTDRLWEFLAFGVVEVSLSLAMLVGLSAVLVYLDWRL
                     GLLMSLPVPALCYAIYRNGEHM"
     gene            complement(1226..2653)
                     /locus_tag="VFQ35_10925"
     CDS             complement(1226..2653)
                     /locus_tag="VFQ35_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931194.1"
                     /translation="MATSEREVGTRGAASRRLQLVVFWGEETAVFDLPLQGDVSIGRA
                     EDNDVRIENPSVSRKHALLRIDDVLSIEDLGGSNGTCVKDARASRDPTQTQGVRRLAR
                     ARAEVAVGDSILLGTVCAVLRHVPEPPAHFPDPGDEPDANSERPNGVVLRDPAMRAVY
                     AEAYGAARATISVLILGETGVGKELLARAIHGRSPRAGGPFVSVNCAAFSESLLEAEL
                     FGYERGAFTGARDAREGLFEAANGGTLFLDEVGEATQATQAKLLRVVEERAVTRIGAR
                     RPRAIDVRIVSATNRDPIADSESGRFRKDLYFRLGGVVLTVPPLRQRPSDIEPLARAF
                     AAAACSQLERGPVELAPETLERLQAYAWPGNVRELRNVIERAVILCADDRLREAQLPP
                     AVRDATPLAAASADATHPRPPEVDAASFKAEVKALDRERIVEALERASGNQTRAAQLL
                     GISRRTLVSRLHEFDLPRPRKRNPR"
     gene            2991..3554
                     /locus_tag="VFQ35_10930"
     CDS             2991..3554
                     /locus_tag="VFQ35_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931195.1"
                     /translation="MARLNVTSLFARLLGVGVIVAGVAASSPAHATGKVGLDLEAALP
                     SDVPDTEQGWGGAARLGNEWNLVLLKLTPEFEANYHKFGGTGDAKQFSALAGGRVGVG
                     LIIQPSVYAHAGVGHFGYRRLYQGMYEDIAQTSFAYDVGAALDLTVLPLIDVGAHASV
                     NGIAGNKDADPFTWYSIGAHVAISFPN"
     gene            3717..>4129
                     /locus_tag="VFQ35_10935"
     CDS             3717..>4129
                     /locus_tag="VFQ35_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931196.1"
                     /translation="MDDTLRHCSGCSGILPGRARFCPSCGRDMQAEDQHPPRAVEPRR
                     ESARRGSGEFEQLPPLRIAPGTRISVYRVEDVIGEGGMGVVYRAYDEALDRVVALKCL
                     HTNLAGDADIRRRFEREARVLRSYSHPGVVSVYDFV"
BASE COUNT          701 a         1365 c         1387 g          676 t
ORIGIN      
        1 ccatgtgctc gccgttgcga tagatggcgt aacagagagc tggcacgggc aagctcatca
       61 ggagccccaa acgccagtcg aggtaaacca agaccgccga caagccgacg agcatcgcga
      121 gtgaaagtga cacttcgacc acaccgaagg ccaagaactc ccacaaacgg tcggtgtcgg
      181 agctcgtgcg agtgatgagg ctccccgtct tcttccttcc gaagaagctc aagctcagcc
      241 ggtgcaggtg cgaatagacc tgatcgcgga ggtcccgtgc cacgaactcg ccgagctctg
      301 ccatcaagcg caagcgcacc cagaggcaga gctggcgcaa cacgtaaacc aagaccatgc
      361 ccccgataca cagccaggcc aggctgctca cgcgcgtggc tgcgacctcg gcgcgttgga
      421 ccgggccgat cactcgatcg accagaaagc ccgtcacgag cggcggcacc atcgaaaaga
      481 cggtgatcgc gaaggcggcg ctcatgcccc agaccagccg tcgtttgtat ggccgcaaat
      541 agcccagcag tcgccacacg accgagagct tgttggccgt caccagcgct tgagcctcgc
      601 ggacctgact cagcatgcta ctcgcgtact cggcggaggc gtcgtgggtc cccacggttc
      661 taccttcgag cgcctgctcg agcagaaact cgatgcgacc catcgcgcgg cgttgtcgtc
      721 gggtgaaata gagctcgacg agcggcgcgc tgcccgtttc gccgtgcaag cggagcacct
      781 ggcaagagag cccgtcctcg ctgctcagcc cgcgcacgtc gtttcgacga aagcattcgg
      841 tgcggtgcgc accaggctcg ccgcgcacga cggcgatgcg ctctcgtccg acgacgaccc
      901 agctctgtga aaggccgagg ccgcggtcga ggtcggccat cgcgaacgcg acgatctccg
      961 gttcgccgaa agccgtctcg acggcgacgc atacggagga cgggagcgtg gcaggctgtg
     1021 cgaggtagcg gtcgaagagc tcgctatccg acaaagatga attgggcatg atggctcgtg
     1081 gcgcacgcgc agagagcgcg cgccgcccca tgccgctttc atgccgacgc gaatctctcg
     1141 caaatccgcc aaattgcgcg gtgcgatggc ctggttgcgc aacgagctgc gcaaaccaat
     1201 tgagcaagtg ttcgcgcgag acgagtcagc gtgggttgcg cttgcgcggc cgaggcaagt
     1261 cgaactcgtg caagcgcgac accaaggtgc ggcgcgaaat gccgagcagc tgcgctgcgc
     1321 gggtctgatt tcccgaggcg cgctcgagcg cttcgacgat gcgctcgcgg tcgagcgcct
     1381 tcacctcagc cttgaagctc gccgcatcga cctcgggagg gcgcggatgg gtggcgtcgg
     1441 cagatgccgc ggctagaggc gttgcgtcgc gtacggcggg ggggagctgc gcttcgcgta
     1501 ggcggtcgtc ggcgcacaag atgaccgcgc gctcgatgac gtttcggagc tctcgaacgt
     1561 tgccaggcca agcgtaggct tgcaggcgct cgagcgtttc cggcgcgagc tcgaccgggc
     1621 cgcgctcgag ctggctgcat gcggccgcgg cgaacgcgcg cgccaacggc tcgatgtcgc
     1681 tcggccgctg gcgcagcggg ggaacggtga gcacgacccc gcccaagcga aagtagaggt
     1741 ccttgcgaaa gcgtcccgac tccgagtctg cgatgggatc gcgattggtc gccgacacga
     1801 tgcgcacgtc gatcgcgcgc gggcgccgag caccgattcg cgtgaccgcg cgctcctcga
     1861 ccacgcggag cagctttgcc tgagtcgcct gggtcgcttc gccgacctcg tcgaggaaca
     1921 gcgttccccc gttcgcggcc tcgaacaagc cctcgcgcgc gtcgcgcgct cccgtgaaag
     1981 cgccgcgctc gtacccgaag agctcggctt ctagtaggct ctcgctgaag gccgcacagt
     2041 tgacgctgac gaatgggccg ccggcacggg gcgagcggcc gtgaatggcg cgggcgagca
     2101 actccttgcc gacgccggtc tcacccagaa tgagcacgct gatcgtcgcg cgggcggcgc
     2161 cgtacgcctc ggcgtaaacg gcgcgcattg ccgggtcgcg cagcacgacc ccgttgggtc
     2221 gctccgagtt cgcgtctggc tcgtctcccg gatcgggaaa gtgcgcggga ggctccggca
     2281 cgtgccgcag caccgcgcac accgtcccga gcagaatgga gtcgccaacc gcgacctcgg
     2341 cgcgcgcgcg tgccaggcgt cgaacgccct gcgtttgggt ggggtcccgg ctcgcccgcg
     2401 cgtctttcac gcaggtgccg ttcgagccgc ccaaatcctc gatgctcagc acgtcgtcga
     2461 tcctcagcaa agcgtgcttc ctcgagacgg aagggttctc gatgcgcacg tcgttgtctt
     2521 cggctcgacc gatcgaaacg tcgccctgga gtggcagatc gaacacggcg gtctcttcgc
     2581 cccagaagac gaccagctgc agcctccggc tcgccgcacc tctcgttccg acttctctct
     2641 cgctggtcgc caccggacac gggtttaccc tcgagcgccg agggtcgtca aaaaggtcac
     2701 gaactccggc gaaattcggc ctccactgcg aggccgagcg gctgcttcgg tgcgcggcgt
     2761 tcacggcccg ggcgcgaagc gtggcgactc gcagagtcgt ttccgagcat gcggaattct
     2821 cggcgtagag gccaaaacgc cccgctgagc gcgctcggcg cgacctcgct tcttcgcgct
     2881 gcaacgcgca cggccacgat gtcgggtttt tccgcatggc acatcgcttg ctctggctct
     2941 catggcgcca ccgaacgagt ggcgagtctt atcaatccaa ggagatatcg atggctcgac
     3001 tcaacgtgac gtcgctcttc gcgcgactgc tcggtgtcgg cgtgatcgtg gcgggcgtcg
     3061 cggcgtcgtc accggctcac gccaccggta aagtaggtct cgatttggag gcggcgcttc
     3121 cttcggacgt tcctgatacg gagcagggtt ggggtggggc cgcgcgcctc ggcaacgagt
     3181 ggaacctcgt tctgctgaag ttgactcccg agttcgaggc caactatcac aagtttggag
     3241 ggacgggcga cgccaagcag ttcagtgcat tggcgggcgg gcgggtcggc gtcggtctga
     3301 tcatccaacc gagcgtctac gcgcacgcgg gcgtcggaca cttcgggtat cgcaggttgt
     3361 accaggggat gtacgaagac atcgcgcaaa cctcttttgc gtacgacgtc ggcgcggccc
     3421 tcgatctcac ggtgttgccg ctcatcgacg tgggcgccca cgcctccgtg aacggtattg
     3481 cgggcaacaa ggacgcagac cccttcacct ggtattcgat cggcgcgcac gtcgcgattt
     3541 cgttccccaa ctgatgtact gatacgactc gtgcgagcgg tccgtcgcgc cgccaacccg
     3601 gggacgggag gtcgagcggc gccgtcgacg cccgcggatc caccggctcc agaggcttcg
     3661 tcgcgcccga tccggcgcga gcgcggtgcg tattgtatgc tcgcgccggt atggccatgg
     3721 acgacactct ccggcactgc tctggatgca gcgggatcct gccggggcgc gctcgctttt
     3781 gcccgagttg cgggcgcgac atgcaggcgg aggatcaaca tcccccgcga gccgtggagc
     3841 cgcgtcgaga gagcgcgcgc cgcggaagcg gggagttcga acagctcccg ccgctgcgga
     3901 tcgcgccggg cacccgcatc tcggtgtacc gggtcgagga cgtcatcgga gaaggcggca
     3961 tgggagtcgt gtaccgcgca tacgacgaag cgctggaccg ggtcgtggcg ctgaagtgcc
     4021 tccacacgaa cctcgctggc gatgctgaca tccggcgccg cttcgagcgc gaagcgcgcg
     4081 tcttgcgttc gtattcgcac cccggcgtgg tcagcgtgta cgacttcgt
//
LOCUS       DASTKS010000528         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_530011, whole genome shotgun sequence.
ACCESSION   DASTKS010000528 DASTKS010000000
VERSION     DASTKS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530011"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VFQ35_10940"
     CDS             complement(<1..1061)
                     /locus_tag="VFQ35_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET9931197.1"
                     /translation="MAVRGFTPRLACVDATRANAERQIAANRGTYDPRPVWTANVAVG
                     SGARGRGFGVLVTLALVSVACAGPEGSSVKDGEPAVAATTLGLSADATVAGVDQITVR
                     VPKRFNVPYDGAARAEFPDGLPLSLGSGLRLSPNRRCRCDASNAEYTLIGLSDRGPNG
                     DAPDYLDAASVKHASKSYLVPDFTPELVTVRVRHGAAVVTHTEELSFGRHAAVGLPPA
                     AFTTEIGVDQSLHALASSADGIDPEGLDFDARGDAWISEEYGPSLLKVNTRSGQILRR
                     LLPSAGLPAILSSRQVNRGFEGVAVAPSGKVYGLVQSTLDVDGKSKGLAQFIRLVELD
                     PRTGATRMFAYPHDVAAYK"
     gene            1349..2530
                     /locus_tag="VFQ35_10945"
     CDS             1349..2530
                     /locus_tag="VFQ35_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9931198.1"
                     /translation="MKYRRFGRTNLNMPVLTCGGMRYQQAWKDLTASEIARENQANVE
                     ATVLRALDLGINHIETARGYGSSELQLGFVLPKLRREDLIVQTKVSPTEKADDFVRSF
                     HTSLERLKLDYVDLFSLHGINNDTIAEWSLKKNGCLDAALKLKEQGLVRHVGFSTHAA
                     PEVILHVIEQGDFEYVNLHYYFVNQRTARCVKAAAARDMGVFIISPNDKGGQLYAPPP
                     KLVELCRPLSPMIFNDLFCLSHPEVHTLSIGASKPGDFDEHIAALAHYENAREVTAPI
                     LERLEATLEATHGREWCERWFEGLPQFQDVPGNINVREILRVYTYAKAFDLSEWAKAR
                     YNLLGRADDWFPGENAASFDDAEVARALAKSPFPERIVQALHEAHAMLHDAPKQRLSQ
                     S"
     gene            complement(2540..2875)
                     /locus_tag="VFQ35_10950"
     CDS             complement(2540..2875)
                     /locus_tag="VFQ35_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931199.1"
                     /translation="MESGAQKEPSCTSCGGSGEIPTDFGPTDCPDCGGAGFLPRKHAL
                     VDWRSRDIERAVAAGIHPAAVDIRWLLAELRSARTALSQVVALAHDVQDEDAIAVKIR
                     LIASRALGL"
     gene            2985..3692
                     /locus_tag="VFQ35_10955"
     CDS             2985..3692
                     /locus_tag="VFQ35_10955"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9931200.1"
                     /translation="MHSAHETDPPDFSGIPDPLVERDVDLTHGVIEGSFRSGLERAGP
                     TRDGLARRRRTLLLASLVWLLALLAGFGVRRDFGALPSSQLLWMEIVPLLGVFLCARL
                     ALSSGAIGLGARTKVLLVAGVAALCVPATLSFLLLPADSGELGARQHLACFGIAFGGA
                     FPPVLLLGIALPHSFVSRPRLRGALLGIAAGLGGVSLVNLHCAANSRVHVGFAHHAPL
                     LALAVIAAAWLGHRMRV"
     gene            3777..4403
                     /locus_tag="VFQ35_10960"
     CDS             3777..4403
                     /locus_tag="VFQ35_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931201.1"
                     /translation="MSAVIPGVWPRVPERAPKLSALPDEELMLAYVLHEDRTAFAELY
                     RRLSPDLMRYAARRIPRESAEDLVQQAFLNAQLARERFALGSAVRPWLTRILVNLVRD
                     HLRARRNHLRSDIDVHELATGPREAVALHESREAIRRARHALSELSPSQRDVLRLHWL
                     EERPFPEVARALGVRLSTAKVRAHRAYKELRRALGDSDETLVRACRKC"
     gene            4413..4688
                     /locus_tag="VFQ35_10965"
     CDS             4413..4688
                     /locus_tag="VFQ35_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET9931202.1"
                     /translation="MSAVLGLSLQIILGIAFPALVIRRDLSHLSAERLALAWNDASLW
                     SAVVTFGPLSMIVHFARTRRSLRGFALGVGWASAGMLASALVGALFG"
     gene            4858..>5214
                     /locus_tag="VFQ35_10970"
     CDS             4858..>5214
                     /locus_tag="VFQ35_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HET9931203.1"
                     /translation="MVAPPRLPPKKDVANALLEQSTVFVHLDPRSDQVKVPPWFKKQP
                     QLVLQVGHNMAVRIPDLDIGDEALSCTLSFNRAPHFCWIPWGAVFALVGENGRGMVWP
                     DDVPKEVAAQAAQQQKK"
BASE COUNT          878 a         1723 c         1709 g          904 t
ORIGIN      
        1 ttcttgtagg cggcgacgtc gtgcgggtac gcgaacatgc gcgtcgcgcc ggtgcgcgga
       61 tcgagctcga ccaagcgaat gaactgcgcg agccccttcg acttgccgtc aacgtcgagc
      121 gtactctgga ctaggccgta gacttttccg gaaggagcga ccgcgacgcc ctcgaacccc
      181 cgattcacct gccggctcga cagaatggcg gggagccccg ccgaaggtaa caaccgccgc
      241 aggatctgcc cgctccgcgt gttcaccttc aacagcgagg gcccatactc ttccgagatc
      301 catgcgtccc cacgcgcgtc gaaatcgagc ccttcgggat cgatgccgtc ggccgacgat
      361 gccaaggcgt gcaagctctg gtcgacgcca atttcggtcg tgaacgcggc cggcggcaag
      421 cccacggccg catggcgccc gaagctgagc tcttcggtgt gcgtaacgac cgccgcgccg
      481 tgccgcacgc gcaccgtaac gagctccgga gtgaagtcgg gcacgaggta gctcttgctc
      541 gcgtgcttca ccgaagccgc atcgaggtaa tcgggagcat cgccgttggg tccgcgatcg
      601 ctcaagccga tcaaggtgta ctcggcgttc gaagcgtcgc agcggcagcg gcgattgggg
      661 ctcaggcgca gcccagaacc caagctgagc ggcaacccat ccgggaactc cgcgcgcgca
      721 gcgccgtcat agggaacgtt gaagcgcttc ggcacgcgca ccgtgatttg gtcgactccg
      781 gcgacggtcg cgtcggcgct gagcccgagc gtagtcgcgg cgacggctgg ttcgccatcc
      841 ttaaccgagg agccttctgg cccggcacac gccacgctca cgagtgcgag cgtcacgagc
      901 acgccgaagc cgcgcccgcg agcgccggaa ccgaccgcaa cgttcgcggt ccacaccggg
      961 cgagggtcgt aagtcccgcg gttcgccgcg atctgtcgtt cggcgtttgc tcgggttgca
     1021 tccacgcaag cgagccttgg tgtaaagcca cgcacagcca tgtcagtcct cctcggttgg
     1081 gcgggcagtg gacgactgct gccccgaagg ggtcaatagg ggaaatttct ggagcgcttc
     1141 gagccgcgaa gtgccgtgct cgccgccatc caccgcggac tttgagaggg cgcgtgtgac
     1201 ggcgcggtta cccactcgcg agatgtgggt gacacttcag gcactcgcgc aggattgctc
     1261 cgttttctga gcgtccttga gcaaaattat gtccgggtca gaaggccagg tcggccgtat
     1321 gtcatgctcc ggcggctcgc tcatgaccat gaagtaccga cgtttcggac gaacgaacct
     1381 aaacatgccg gttttgacct gcggaggcat gcgctaccag caggcctgga aggatctcac
     1441 cgccagcgag atcgcgcggg agaatcaggc caacgtcgag gcgacggtgc tgcgcgcctt
     1501 ggacttgggc atcaaccaca tcgagaccgc ccgcgggtat ggctcttcgg agctccagct
     1561 cgggttcgtc ttgccgaagc ttcgacgcga agatctcatc gtgcagacga aggtgtcgcc
     1621 gacggagaag gccgacgatt tcgtgcgtag cttccacacg tcgctcgagc gactcaagct
     1681 cgattacgtc gacctcttct cgctacacgg aatcaacaac gacaccatcg ccgagtggtc
     1741 gctgaaaaag aacggatgcc tggacgcagc gctgaagctg aaggagcaag ggctcgtgcg
     1801 ccacgtcggc ttttcgacgc atgcggcgcc cgaagtcatc ttgcacgtca tcgagcaggg
     1861 cgatttcgag tacgtgaact tgcactacta cttcgtgaac cagcgcacgg cgcgctgcgt
     1921 gaaagcagcg gccgcacgcg atatgggcgt cttcatcatc agcccgaacg acaagggcgg
     1981 acagctctac gcgccgcctc cgaagctcgt cgagctctgc cgcccgctct ccccgatgat
     2041 cttcaacgat ctcttctgtc tgtcacaccc cgaggtgcat acgttgagca tcggcgcgtc
     2101 gaagcccggc gatttcgacg agcacatcgc agcgctcgcg cactacgaaa acgctcgcga
     2161 ggtcacagcg ccaatcctgg agcggctgga ggcgacgctc gaggcgactc acggccgcga
     2221 atggtgtgag cgctggttcg aggggctgcc gcagtttcaa gacgttccgg gcaacatcaa
     2281 cgtgcgggag atcctgcgcg tgtacactta cgccaaagcg ttcgacctga gcgagtgggc
     2341 caaggcgcgc tacaacctgc tcgggcgcgc cgacgactgg ttcccgggcg agaacgcggc
     2401 aagcttcgac gacgccgagg tcgcgcgggc gctcgcaaag agcccgttcc cagagcgcat
     2461 cgtccaggcg ctgcacgaag cgcacgccat gctgcacgac gcgcccaagc agcgcctcag
     2521 tcagagctga agcgacgcct cacaggccga gcgcacgcga cgcgatcagc cgaatcttta
     2581 cggcgatcgc gtcctcgtcc tggacgtcgt gcgcgagcgc tacgacctgg ctcaacgccg
     2641 tacgggccga cctgagctcc gcgagcagcc agcggatgtc gacggccgcc ggatggatcc
     2701 ccgccgcgac cgcccgctcg atgtctcgcg agcgccagtc gaccagcgcg tgctttcgcg
     2761 gcaaaaagcc ggcgccaccg cagtcggggc agtcagtggg gccgaaatcg gtcgggattt
     2821 cgcccgaacc tccgcaactc gtgcagctgg gctccttttg agctccgctt tccatgctgg
     2881 tgggagtacc acaacttgca cgcctctggc ggacctcgca acgaagaaga aacgggcgcc
     2941 ctgattcgtc gtagagtggg cagaagcgga aagtagcctc gcccgtgcac tctgctcacg
     3001 agaccgaccc ccctgatttt tcgggaattc ccgatccgct cgtcgagcgc gatgttgatt
     3061 tgacgcacgg ggtcatcgaa ggctcgtttc gctccgggct cgagcgagct ggcccaacac
     3121 gagacgggtt ggcccggcgc cgacggacgc tgctgctcgc ctcgctggtc tggctgctcg
     3181 cgctgctggc cggtttcggg gtgcggcgcg acttcggagc cctcccgtcc tcgcaactcc
     3241 tgtggatgga gatagtgccc ctgctcgggg tgttcctctg cgcccggctc gcgctctcga
     3301 gcggcgcgat cggtttggga gcgcggacca aagtactgct cgttgcggga gtcgccgcgc
     3361 tgtgcgtgcc agcgaccttg agcttcttgt tactgccagc cgactccggc gagctcggag
     3421 cacgacagca cctcgcatgc ttcgggattg cattcggggg cgcattcccc ccggtattgc
     3481 tgctcggcat tgcgttgcca catagcttcg tgtcgcgccc gcgattgcgt ggggcgttgc
     3541 tcggcatcgc ggccggcctc ggaggcgtgt cgctcgtgaa cctgcattgc gccgccaatt
     3601 cgcgcgtgca cgtcggcttc gcgcatcacg cgccactgct cgcgctcgcg gtgatcgctg
     3661 cggcgtggct cgggcaccga atgcgcgtgt agagaaaatc gaaaaccggg agcacacgtc
     3721 ggcgtagtga ggctgcgcgc cgcaaaacga aacgacgtcg cgcgcaggag tcgaacatgt
     3781 ccgcagtgat acccggagtg tggccccgtg tgccggaacg cgcgccgaag ctctcggcgt
     3841 tgcccgacga agagctgatg ctcgcttacg tgctccacga ggaccgcacg gcgttcgccg
     3901 agctctatcg ccggctctca ccagacttga tgcggtacgc agcgcgacgt atcccacgcg
     3961 agagcgccga agatctggtg cagcaggcat tcttgaacgc gcagctcgcg cgcgaacgtt
     4021 tcgcgctcgg gagcgcggtg aggccctggc tcacgcgtat cttggtgaac ttggtgcgag
     4081 atcacttgcg cgctcgtcgc aatcacctgc gctcggacat cgacgttcac gagctcgcga
     4141 cgggaccgcg cgaagccgtg gctctgcacg agagccgcga ggccattcgg cgcgcgcgcc
     4201 acgcgctgtc ggagctctcc ccttcgcagc gcgacgtgct tcgcttgcac tggctcgaag
     4261 agcgaccgtt ccccgaggtc gcgcgcgcgc tcggagtgcg cttgtcgacg gccaaggtgc
     4321 gcgctcatcg cgcctacaag gagcttcggc gcgcgctcgg cgactccgac gagacgctgg
     4381 ttcgagcgtg ccggaaatgc tagcgatact gcgtgtccgc ggtgctcggg ctctcgctcc
     4441 agatcattct gggcattgcg ttccccgcgc tcgtcattcg ccgcgatttg tcacatcttt
     4501 cggcggagcg gctcgccctc gcctggaacg acgcgagcct atggtcggcg gtggtcacgt
     4561 tcggcccgct cagcatgatc gtccacttcg cacgcacgcg ccgctcgctg aggggcttcg
     4621 cactcggagt cggctgggcc agcgccggga tgctcgcgtc ggcgctcgta ggagccctgt
     4681 tcggctgagc ttcgggcacg cggcgcgccc cgccagggag tcggcgcctt cgcctgcgca
     4741 gatgttctcg acgagacggg gagccgcccc gccgattcgg cgaaagccct acccccgagg
     4801 ggccagaaat gccggggggt ctggccttcc gggcgcggcg gcgctattga gcgggtgatg
     4861 gtcgcgcccc ctcggctgcc cccgaagaaa gacgttgcga acgcgctcct cgagcagtcg
     4921 acggtattcg ttcacctgga tccgcgttcg gaccaagtga aggttccgcc ctggttcaag
     4981 aagcagcccc agctcgtgct tcaggtcggg cacaacatgg ccgtccgcat tccggatttg
     5041 gacatcggcg acgaagcgct ctcttgcact ttgagcttca accgcgcgcc ccacttctgt
     5101 tggattcctt ggggggcggt cttcgcgctc gtcggggaaa acgggcgcgg catggtttgg
     5161 cccgacgatg tgccgaagga agtcgcggcg caggcagcgc agcaacagaa gaag
//
LOCUS       DASTKS010000529         3925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_530567, whole genome shotgun sequence.
ACCESSION   DASTKS010000529 DASTKS010000000
VERSION     DASTKS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530567"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFQ35_10975"
     CDS             complement(<1..571)
                     /locus_tag="VFQ35_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9931204.1"
                     /translation="MKIFITGASSGIGEALARAYSRPGAHFGLVARRRELLEKLAAEL
                     RGTGAAVSVYPLDVTDTQAMHAAASDFVERAQGIDRVIANAGIGIGDTLRDGDSESVR
                     HLMEVNVIGVTNTIVPFIPVALRQGFGTLVAVSSMAGHRALPGRAAYSASKAAVITFM
                     DALRMQLVGTGVHSMTLCPGFVHTPLTETL"
     gene            complement(574..1806)
                     /locus_tag="VFQ35_10980"
     CDS             complement(574..1806)
                     /locus_tag="VFQ35_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9931205.1"
                     /translation="MGQLDTNAYYALGAPMYGLLVAVEYWVASRRGVRVFGFADSIGN
                     ISAGVGEVIVGLFLGPLLIALYDFGYDHIALVHWPERSWVPWVLAFLVSDFCYYWYHR
                     AGHRVAVLWSIHGVHHQSREFNVTVAMRHPWLSDSYSALFYVPCPLLGIPPTHFFVAI
                     SLISFYALTIHTRFFNRPSLYVLTTPATHVLHHATNPRYLGKNLGAMFTIWDRLFGTY
                     AAPDATEPPVLGSLGGYQSHDGAWAQWQFPRLLFHGARRARTFGEVLRVFFSHPGWLP
                     RECTPPKLPAARDDAQIPRSLKTYIATQFGVLLAFAIWVSWLRAHHGWALLLASSLLI
                     LFGLGALGGLLDGRRGARQVELIRLLANSSFAFTLAVVGRDPFSLVYVGFALLSARWF
                     VSIREDAPAAHHHFESRG"
     gene            complement(1871..2656)
                     /locus_tag="VFQ35_10985"
     CDS             complement(1871..2656)
                     /locus_tag="VFQ35_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931206.1"
                     /translation="MNWLHSTEAWTSRLLGAALVLQTLELVQIRAALRDDGVFAWPVL
                     RREHARLPALVRALFAPFLPYSRFVVLAWFRLGLALVLLAGESRAAPFLLFTQVAVCV
                     RFRGTFNGGSDYMTVLVLLGLTLPALFGSSPLVATAGYCYVAVQLVFSYFIAGLVKWR
                     EPSWRSGTALARFTNDPRFRSPRWARALFEKPRVGTLACWAVIAFECTFPVALVDGRA
                     TLAYAIFGATFHLFNVVVFGLNRFFFAWSAAYPALFLASHLRG"
     gene            complement(2653..3144)
                     /locus_tag="VFQ35_10990"
     CDS             complement(2653..3144)
                     /locus_tag="VFQ35_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931207.1"
                     /translation="MLVSRALYIGVGLVLLLLAAVALRPRRELSGAGWELFRCFWPSW
                     RFFDSADALPILRARAGRSEEELGPFRDVLRAPRRGPSTLLLNGAFNLELQYFSCVEA
                     LARESELVDVSEVRGLISYELVRRLVESRLLESESVYQFALCEPKGGDAHLVSELHTR
                     GGP"
     gene            3312..>3925
                     /locus_tag="VFQ35_10995"
     CDS             3312..>3925
                     /locus_tag="VFQ35_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9931208.1"
                     /translation="MANDLEGRVILLTGATEGIGKAAAHELSRRGATLVLVGRNREKG
                     ERLVGELKGSSKNSNIDLLIGDLSSLADVRAVAEAFRRKYDRLHVLVNNAGALFRDYV
                     LSKDGIEMTFALNHMGYFLLTRELLELLKSTPGARIVCTSSHAHRGGRIDVDTISTRP
                     DGRAGFRAYCDSKLANILFTKELARRLLGTNVSANCFHPGFVKSG"
BASE COUNT          849 a         1196 c         1312 g          568 t
ORIGIN      
        1 gcagcgtttc ggtgagcggc gtgtgtacga agcctgggca gagcgtcatc gagtgaaccc
       61 cagtgccaac gagttgcatg cgcagcgcat ccatgaacgt gatgacggct gccttcgagg
      121 cagagtatgc ggccctgccc ggtagcgcac ggtgcccggc catggagctt acggcaacga
      181 gcgttccgaa tccttggcgg agcgccaccg gaatgaacgg aacgatggtg ttcgtgacgc
      241 cgatcacgtt gacctccatc aagtgcctaa cggattcgga gtcgccatcg cgcagagtat
      301 ctccaattcc gattccggcg ttcgcgatga cgcgatcgat gccctgagcg cgctccacga
      361 agtcgctcgc ggccgcgtgc atggcttggg tgtccgtcac gtcgagcggg tacacgctga
      421 cggcggcacc agtaccgcgg agctctgccg cgagtttctc gaggagctca cgccgcctcg
      481 ccacgaggcc gaagtgggcg ccggggcggg agtaagcgcg tgcgagggct tccccgatcc
      541 cgctgctcgc tccagtaatg aagatcttca tggtcatcct cggctttcga agtggtgatg
      601 agcggcgggc gcgtcctcgc gaatcgagac gaaccagcgc gcgctgagca acgcgaagcc
      661 cacgtacaca agcgaaaacg ggtcgcgccc tacgacggcg agcgtgaacg caaacgacga
      721 attcgccaac aggcgaatca attcgacctg acgggcgccg cgccgcccgt cgagcaggcc
      781 cccaagcgcc ccgagtccaa ataggatgag cagcgagctc gcgaggagaa gcgcccaacc
      841 gtggtgagcg cgaagccagg acacccaaat cgcgaaggcg agcaacacgc cgaattgagt
      901 cgcgatgtac gtcttgagcg agcgggggat ctgcgcatcg tcacgagctg ccggcagttt
      961 gggcggcgtg cactctcgtg ggagccagcc cggatgcgag aagaagacgc gcagcacctc
     1021 accgaacgtt cgcgcgcggc gcgcgccgtg gaacagcaag cgagggaact gccattgggc
     1081 ccaagcgcca tcgtggctct gatacccgcc gagcgagccg agaaccgggg gctcggtggc
     1141 gtcgggggcg gcgtaggtgc cgaataggcg atcccagatg gtgaacatcg cgcccaggtt
     1201 tttgcctaaa taacgcggat tcgtcgcgtg atggaggacg tgggtggcgg gggtggtgag
     1261 gacgtacagg ctcggacgat tgaaaaagcg cgtgtggatg gtgagcgcgt aaaacgagat
     1321 caacgaaatc gcgacgaaga agtgcgtggg tgggatgccg agcaacgggc acggaacgta
     1381 gaacagcgcc gaatacgaat ccgacagcca agggtggcgc atcgcgaccg tgacgttgaa
     1441 ctcccgactc tggtggtgaa ccccatggat cgaccacagc acggccacgc ggtgccctgc
     1501 gcggtgatac cagtagtaac aaaagtccga caccaagaag gcgagcaccc aaggaaccca
     1561 cgagcgttcg ggccagtgca cgagcgcgat gtgatcgtac ccgaagtcgt agagcgcgat
     1621 gaggagcggc cccaaaaata ggccgacgat gacctcgccg acgcccgccg atatgttccc
     1681 gatgctgtcg gcgaacccaa agacacgcac tccccgtcgt gaggcgaccc agtactcgac
     1741 cgctaccaag aggccgtaca taggcgcgcc cagcgcatag taagcgttgg tgtcgagctg
     1801 ccccatcgct cattcggtgc ctggccgacg gccgggcgtc aagacggcag gtgtctaccc
     1861 gctgaaaccc ttatccgcgg aggtggctcg cgaggaagag agcggggtag gcggcgctcc
     1921 aggcaaagaa gaagcgattg agtccgaaca cgacgacgtt gaagaggtga aacgtggcac
     1981 cgaaaatggc gtaggcgagc gtcgcacgcc cgtcgacgag agcgacggga aacgtgcact
     2041 cgaaggcgat caccgcccag caggcgaggg taccgaccct cggcttttcg aagagagcgc
     2101 gtgcccaacg cggtgagcga aagcgtggat cgttcgtgaa tctcgccaac gccgtgcccg
     2161 agcgccagct cggctcccgc catttgacga gcccggcgat gaagtacgaa aacacgagct
     2221 gcaccgcaac gtaacaatag cccgccgtcg ctacgagcgg agacgagcca aacagggcgg
     2281 gcagcgtgag ccccagcagt acgagcacgg tcatgtagtc actgccgccg ttgaaggtgc
     2341 cgcgaaaccg gacgcaaacc gcaacttgcg tgaagagcaa gaaaggcgcg gcgcgcgact
     2401 cacctgcgag caagaccaaa gccagaccaa gccgaaacca cgcgagtacg acgaacctcg
     2461 aatagggcag aaacggtgcg aacagcgcgc ggacgagcgc cggaagccgg gcatgttctc
     2521 ggcggagaac cggccatgcg aacacgccgt cgtcgcgcag ggcagctcga atctgcacaa
     2581 gctcgagggt ttgcagcacg agggccgcgc cgagtaggcg tgacgtccac gcctcggtcg
     2641 agtgaagcca attcacggac cgcctcgcgt gtgaagctcc gaaacgaggt gagcgtcccc
     2701 gcccttgggc tcgcagagcg cgaactggta cacgctttcg ctctcgagca atcgcgattc
     2761 gaccaggcgg cgcacgagct cgtacgaaat cagcccccgt acctcgctta cgtcgacgag
     2821 ttccgattcg cgggccagag cttcgacgca agagaagtac tggagctcca ggttgaaggc
     2881 gccgttcagg agcagggtgc ttggcccacg cctgggcgcg cgcagcacgt cgcggaaagg
     2941 gcccagctct tcttcgcttc ggccagcccg ggctcgtaaa atgggaagcg catcggcgct
     3001 gtcgaaaaat cgccaagagg gccagaagca gcggaaaagc tcccagccag cgcctgacag
     3061 ctctcgccga gggcggagcg caaccgccgc cagcagcaga agcacgagtc caacgccgat
     3121 gtagagcgct ctcgagacga gcatggtgac gtgagccctc gaccgcgcgt gagcctcgcg
     3181 ctttcccgaa tgtatcgcgc ggtccggcga cgccgcaaca aaaccgcccc cgcgcaccaa
     3241 cgcggtagta tttaggcgac tccgccgacg aggctcgatg gacggcgggt gacgaggtaa
     3301 aagctccgtc gatggcgaac gatctggaag ggcgagtcat tcttctgacc ggcgcgaccg
     3361 agggaatcgg aaaggcggct gctcacgagc tgagtcgacg cggcgcgacg ctcgtgctcg
     3421 tgggccgcaa ccgcgagaag ggggaacgtt tggtcggcga gctgaagggg tcgagcaaga
     3481 actcgaacat cgacctcctg atcggagatt tgtcgagcct cgcggacgtt cgcgctgtcg
     3541 cggaagcgtt tcgaagaaag tacgatcggc tgcacgtgct cgtgaacaac gcgggtgcgc
     3601 tcttccgaga ctacgtgctc agcaaggacg gcatcgagat gacgttcgcg ctgaaccaca
     3661 tgggctattt cctgctcacg cgagagctac tggagctctt gaagagtacg ccgggtgccc
     3721 gcatcgtgtg cacgtcgagt cacgcgcatc ggggcgggcg tatcgacgtc gacaccatat
     3781 cgacccggcc cgatgggcgc gcggggtttc gcgcctactg cgactcgaag ctcgccaata
     3841 tcctgtttac gaaagaactc gcgcgccgac tgctcggcac gaacgtcagc gcgaactgct
     3901 ttcatcccgg gttcgtgaag agcgg
//
LOCUS       DASTKS010000530         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1199383, whole genome shotgun sequence.
ACCESSION   DASTKS010000530 DASTKS010000000
VERSION     DASTKS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199383"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1371
                     /locus_tag="VFQ35_11000"
     CDS             280..1371
                     /locus_tag="VFQ35_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9931209.1"
                     /translation="MKRPPKKRTSLAPSLRPEPPVENVFLALLSSVQKKVDARLGDFL
                     EQRLAQASSLGPEVKEMVAALGDLSRRGGKRLRPALLVTGYRAVSRRESSAALDAGVA
                     LELLQSYFLIHDDWMDGDLVRRGGPSVHAVLSRSLRDEHKGAASAILAGDYAAALALD
                     VLTRIRLPRSTSAALFGAFAEMQLSAVAGQQIDLVGKARDIEAAYELKTGSYTVYGPL
                     RMGAILAGGSPRLLSKLERLSRPLGVGFQLRDDLLSAFGDPKRTGKPLGNDLKAGKRT
                     LLLAESLRRARGKDRVALASVVGNARAKDSVVKAALEVMDSCGARAWVESRVAELAAE
                     ARSELGAGISSEGNALLEGAISALTERRA"
     gene            1379..2287
                     /gene="mvk"
                     /locus_tag="VFQ35_11005"
     CDS             1379..2287
                     /gene="mvk"
                     /locus_tag="VFQ35_11005"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET9931210.1"
                     /translation="MARANGKVILLGEHAVVYGVPAIAAGIGKGATAIAVPAERPLLV
                     LGAERVVRAGEDDELGRAFTALHAATGVGSYRIDVTLELPPGAGLGASASIGVATARA
                     LFEASGIEAGRERVLEAAGAWERVFHGNPSGIDAEAAACEACILYSRADGARPIAVAG
                     EVPLVIGIAGPSASTRVMVEGVAELRQRKPEVVDKAMAGILALVNAAKGCLGVGDLRG
                     LGRLMDLNQMLLSGLFVSTEGIERACAVARAAGALGAKLTGSGGGGAVIALAPGDPEV
                     ILSAFRSEGIQCFATTVGASSGGTRR"
     gene            2284..>3138
                     /gene="mvaD"
                     /locus_tag="VFQ35_11010"
     CDS             2284..>3138
                     /gene="mvaD"
                     /locus_tag="VFQ35_11010"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET9931211.1"
                     /translation="MSRASEAVACSNIALVKYWGKSARGQNLTAVPSLSLTLDALRTT
                     TRVRFDDELPEDRVKLGDDWVSGRPLERAVRLLDEVRELAAVRTRAEVISRNDFPTAS
                     GLASSASGFAALALAASSALGLGLSNAEISRLARRASASAARSLFPGFVQLLAEAESA
                     EPVAPADWLDVRMLVAVTSLGPKGTLSTDGMLHTQATSPYYPAWVASAPAIFERARAA
                     LLARDLEVLGQAMEQSTLLMHASMMAAEPSLIYLRAATLSVMERVRALRSEGAHAFFT
                     MDAGPHVKV"
BASE COUNT          453 a         1023 c         1122 g          540 t
ORIGIN      
        1 gcaaggcgtc gtgcacgagc cgcacacatt cgaacagggt ggtcttctcg tggaagccca
       61 cgtcgccttt agcggcgaga tccaggtgat ctgctttgcg tttcgcgatg tcgctcatgt
      121 cgtccttggc gcgctcgcgc ccgaatctcg aagctctgaa gcatgggctc ggtccggcca
      181 gcgtgacaac ccccttggac gccgattgtt cgcgtgagct ctcgcgcgtt ggcggcgctg
      241 tcacgaggtc ccgaaaagtg cgctaggctg ccgccgccca tgaagcggcc cccaaaaaaa
      301 cggacttcgc tcgctccctc gctgcgcccg gaacccccgg tggagaacgt ctttttggcg
      361 ctgctctcgt ccgtgcagaa gaaggtcgac gcgcgtctcg gcgacttcct ggagcaacgt
      421 ctcgcccaag cttcgtcgct cggtcccgag gtgaaggaaa tggtggccgc cctcggggac
      481 ttgtcacgcc gcggcggcaa gcggctccgc cccgcgctgc tggtgaccgg ctatcgcgct
      541 gtctcccgcc gtgagtcgag cgcggcgctc gatgcggggg tcgccctcga gctcttgcag
      601 tcgtattttc tcatccacga cgactggatg gacggcgacc tcgttcggcg gggcggcccg
      661 agtgtccacg ccgtgctttc gcgaagcctg cgcgacgagc acaaaggcgc cgcgagcgcg
      721 atcttggcgg gcgactacgc tgcggccttg gcgctcgacg tgctcacgcg gattcgccta
      781 ccgcgctcga cgagcgcggc gctgttcggc gcgttcgccg aaatgcagct ctcggccgtg
      841 gccgggcagc aaatcgatct cgtcggaaag gcgcgcgaca tcgaagccgc ttacgagctc
      901 aagaccggca gttacaccgt ctacggtccg ctccgcatgg gcgccatcct ggccggtggc
      961 tccccgcggc tgctttcgaa gctcgagcgg ctttcgcgtc cgctcggcgt cggtttccag
     1021 ctgcgcgatg acctgctgag cgcgttcggg gatccgaagc gcaccggaaa gccgctcgga
     1081 aacgatttga aggccggcaa gcgcacgctg ctgctcgccg aatcgctgcg tcgagcccgt
     1141 ggcaaggatc gggtcgcgct cgcctcggtc gtcgggaatg cgagggcgaa agactcggtg
     1201 gtgaaagccg ccctcgaggt gatggactcg tgcggtgccc gcgcctgggt cgaatcgcgc
     1261 gtcgcggagc tagcggcaga ggctcggagc gagctcggcg ccgggatttc gtcagaaggc
     1321 aatgcccttc tcgaaggtgc catctccgcg ctcaccgagc gtagggcata gttccatcgt
     1381 ggctcgcgcg aacggaaagg tgatcctgct cggtgagcac gccgtcgtgt acggcgtgcc
     1441 agccatcgcc gccgggatcg gaaaaggagc gacggccatc gccgttcccg cggagcgacc
     1501 gctcctcgtt ctcggcgcgg agcgcgtggt gagggcggga gaagacgacg agcttgggcg
     1561 cgcttttacc gccttgcacg ccgcgaccgg tgttggctcg tatcgaatcg acgtgacgct
     1621 cgagttgccg cccggggcgg gccttggtgc ctcggcgtcg atcggcgtcg cgaccgcgcg
     1681 cgcgctgttc gaggcgtccg gcatcgaggc aggccgagaa agagtgttgg aagccgcggg
     1741 cgcgtgggag cgagtctttc acggcaaccc atcgggcatc gacgccgagg cggccgcgtg
     1801 cgaggcgtgc atcctctatt cgcgtgcgga cggcgctcgg ccaatcgcgg tggctggcga
     1861 ggtgccgctc gtcatcggta tcgccggccc ctcggcgagc acgcgggtga tggtggaggg
     1921 cgtcgccgag ctgcgacaac gcaagccaga ggtggtcgat aaggcgatgg ccggcatcct
     1981 cgccttggtg aacgcggcca agggatgcct cggcgttggc gatttacgcg gcctcgggcg
     2041 cttgatggat ttgaaccaga tgctgctctc cggattgttc gtttcgaccg aaggtatcga
     2101 acgcgcttgt gcggtcgcgc gcgcggcggg tgcgctcggc gcgaagctca ccgggtcggg
     2161 cggggggggc gccgtcatcg cgttggcccc gggagatccg gaggtgattc tctcggcgtt
     2221 tcgcagtgag ggcattcagt gcttcgcgac caccgtcggc gcgagctccg gaggaactcg
     2281 acgatgagcc gggcgagcga ggcggttgcg tgctccaaca tcgccctcgt caagtattgg
     2341 ggcaaatcgg cgcgcggaca gaacctgacg gcggtgccga gcttgtcgct cacgctcgac
     2401 gcgctccgca cgacgacgcg tgtgcgcttc gacgacgagc tccccgaaga ccgcgtcaag
     2461 ctcggggacg actgggtgag cgggcggcca ctcgaacgcg ccgtgcgctt actcgacgaa
     2521 gtgcgagagc tcgcggcggt gcggacgcgc gcggaggtga tttcacggaa cgactttccg
     2581 accgcttcgg ggctcgcgtc gagcgcttcc gggttcgcgg cgctcgcctt ggccgcgtcg
     2641 tccgcgctcg ggctcggttt gtcgaacgcc gagataagcc ggctcgcgcg gcgtgcgtcg
     2701 gcgtcggcgg cgcgttcgct ctttccgggc ttcgtgcagc tcctggccga ggccgagagc
     2761 gccgagcccg tcgctcccgc cgactggctc gacgtgcgca tgctcgtcgc ggtcacgagc
     2821 ctcggcccga agggtacact ctcgaccgac ggcatgctcc acactcaggc aacgagtccg
     2881 tactacccag cctgggtggc gagtgcgccc gccatcttcg agagagcgcg cgcggcgctg
     2941 ctcgcccgcg atctcgaggt actcggtcag gcgatggagc agagcacgct actcatgcac
     3001 gcgagcatga tggcggccga gccgagcctc atctacctgc gcgccgcgac gctctcggtg
     3061 atggagcgcg tgcgcgcgtt gcggagcgag ggcgctcacg cattcttcac gatggacgcg
     3121 gggcctcacg tgaaggtg
//
LOCUS       DASTKS010000531         3684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_752485, whole genome shotgun sequence.
ACCESSION   DASTKS010000531 DASTKS010000000
VERSION     DASTKS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752485"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFQ35_11015"
     CDS             complement(<1..218)
                     /locus_tag="VFQ35_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9931212.1"
                     /translation="MTNSAETQASLVESGRRFLYPNYRQPALVIDRGDGAVLWDVAGK
                     RYLDFFAGIAVSALGHAHPALVRALAEQA"
     gene            complement(215..1426)
                     /locus_tag="VFQ35_11020"
     CDS             complement(215..1426)
                     /locus_tag="VFQ35_11020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HET9931213.1"
                     /translation="MPSSPFSEIPLAPPDPILGLTEAFQADKNPQKVNLGVGVYQDGN
                     GKVPVLKVVREAEKRWYEKEDSKSYLPIDGVPTYTAEVKRLLFGNDSPLIKEGRAVTA
                     QALGGTGALKLGADFLKRWVPGANLYISTPSWENHRAVFESAGFTVGEYTYYDAQKQS
                     VDFEGLLASLNKLAPKSIVLLHACCHNPTGVDLEAGQWTQVANLMKERGLIPYVDFAY
                     QGFGAGISEDAVAVRAFSEAGAPFLIATSFSKSFSLYRERVGAITLVAESADEAKRVT
                     SQLKRVIRTNYSNPSSHGAQVVALILSDAELRKQWEAELTEMRERIQRMRSLFVERLA
                     AKGVKRDFSFVKRQNGMFSYSGIALEPVRRLRSEFGIYIVDSGRICLAALNEKNVDYV
                     TDSIAKVLASA"
     gene            complement(1491..2270)
                     /locus_tag="VFQ35_11025"
     CDS             complement(1491..2270)
                     /locus_tag="VFQ35_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET9931214.1"
                     /translation="MQPASALSSERARGLRGLLFDLDDTLLDHGRLLPAALTALESMK
                     KTGLELFAVTGRPAGWGAVITHQWPIDGAITENGGIAIVRDGARVTLRDAVAPLERER
                     RRAELAALVSVLRREYPDLVPSDDVWQRITDYTFDIGEHQRVPRDVVLRVRRRAHELG
                     ATTIASSVHLHVSLDRADKASASCQLMAALYGIDPFRVLSSYAFIGDSENDAACFNAF
                     QVTFGVKNLSGRPTLSPRFLTRGERAAGFVEAAELLLARRA"
     gene            complement(2270..3469)
                     /locus_tag="VFQ35_11030"
     CDS             complement(2270..3469)
                     /locus_tag="VFQ35_11030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9931215.1"
                     /translation="MSIGYVTLRAGHVQPVWAGHPWVFAQAVERIEGGVRAGDEVVVR
                     DARGNPLGRGLYSPGSAIPVRLYTRDANRSIDEGLLAERISKAIQRRRVLGLPDDRTT
                     GFRLIHAEGDDLPGLVVDQLGDVLSLQFGTVGLKQRENIVLSLLERALSPRAIVDRTP
                     ERTAASEGFRPGTGVVRGDESLAAFEFSERDLRFRVPLELGQKTGFYFDQRPLRERVE
                     SLARGRRVLDLYSYTGAIALSAARGGAVSVEAVDTSAVALEVAAECAARNGLSGKVRF
                     ECGDASAALKRVGAEGGADLVIVDPPKLAPSRARRERALEAMRRLCAAGARATEPNGL
                     LIVSSCSAALGMMDLTRSLALGARDVGVRATILERIYQGPDHPVPAAFPEGLYLSTVI
                     AQVTGSA"
BASE COUNT          621 a         1220 c         1225 g          618 t
ORIGIN      
        1 gcctgctcgg cgagcgcgcg cacgagcgcc gggtgagcgt ggcccagcgc gctcacggca
       61 atgcccgcga agaagtcgag gtagcgcttg ccggcgacgt cccacaagac cgcgccgtcg
      121 ccgcggtcga tgacgagcgc cggctgacga taattcggat acaggaagcg tcgcccgctt
      181 tcgacgagcg acgcttgggt ttcggccgag ttggtcatgc ggaggcgagc accttggcga
      241 tgctgtcggt gacgtagtcg acgttcttct cgttcaaggc agcgaggcag atgcggccgc
      301 tgtcgacgat gtagatgccg aactcggagc gcagcctacg cacgggctcg agcgcgatgc
      361 ccgagtacga gaacatgccg ttttggcgct tcacgaagga gaagtcgcgc ttcacgccct
      421 tcgcggcgag gcgctccacg aacaggcttc gcatgcgttg gatacgctcg cgcatctcgg
      481 tgagctcggc ctcccactgc ttacggagct cggcgtccga caagatgagc gcgacgacct
      541 gcgcgccatg cgagctcggg ttcgagtagt tggtgcggat gacgcgcttc aactgcgacg
      601 tcacgcgctt ggcctcgtcc gcgctctccg cgaccagcgt gatggcgccg acgcgctcgc
      661 gatagagcga gaacgacttc gagaacgacg tcgcgatcag gaacggcgcg cccgcttcgc
      721 tgaatgcgcg caccgcgacc gcgtcttcgc tgatgcccgc gccgaagccc tggtaagcga
      781 agtcgacgta cgggatgagt ccgcgctctt tcatcagatt cgcaacctgg gtccactgtc
      841 ccgcctcgag atcgaccccg gtcgggttgt ggcagcaggc gtgcaggagg acgatgctct
      901 tgggcgcaag cttgttgagc gatgcgagca gcccttcgaa gtcgacgctc tgcttctgcg
      961 cgtcgtagta agtgtactcg ccgacggtga agccagcgga ctcgaacacg gcccgatggt
     1021 tctcccagct cggcgtgctg atgtagaggt tcgctcccgg cacccagcgc ttcaagaagt
     1081 cagcgccgag cttgagcgcg ccggtgccac cgagcgcctg cgccgtgacg gcgcgtccct
     1141 ccttgatgag cggagagtcg ttcccgaaga ggagccgctt gacctcggcg gtgtacgtgg
     1201 ggacaccgtc gatgggcaaa taggacttcg agtcctcttt ttcataccag cgcttctccg
     1261 cctcgcgcac caccttcagg accgggacct tgccattccc gtcttggtag acgccgacgc
     1321 ccaggttcac cttctggggg ttcttgtcgg cctgaaaagc ttcggtgagc ccgagaattg
     1381 ggtcaggggg agcgaggggg atttcggaaa acggagacga tggcatggtc ggatttcctc
     1441 gaaaggtccg gtttgttgcc ggaaccttga cccgcatccc tctagcaaag ctaggcccgg
     1501 cgtgcgagca agagctccgc ggcctcgacg aagcccgccg cccgctcccc ccgggtgagg
     1561 aagcgcgggg ataaagtcgg acgaccgctc aggtttttga ccccgaacgt gacctggaag
     1621 gcgttgaagc acgctgcatc attttcgctg tcaccgatga aggcgtagct cgacagcact
     1681 cggaaggggt cgattccata cagcgccgcc atcaattggc acgaggcgct cgccttgtcg
     1741 gcgcgatcca agctcacgtg caggtgaacg ctcgaggcga tcgtcgtggc tccgagttca
     1801 tgggcgcgcc gcctgacgcg aagtacgacg tcgcgcggca cgcgctggtg ttcgccgatg
     1861 tcgaaggtgt agtcggtgat gcgctgccac acgtcgtcgc tcggcacgag gtcggggtat
     1921 tcgcggcgca gcaccgagac gagcgcggcg agctcggctc ttcggcgctc gcgctccagc
     1981 ggcgcgacgg cgtcgcgcag cgtgacacgc gcaccgtcgc ggacgatggc gatgccaccg
     2041 ttttcggtga tggctccgtc gatcggccat tgatgcgtga tcacggcacc ccagccggcg
     2101 ggtctgcccg tcaccgcgaa gagctcgagc cccgttttct tcatgctctc gagtgcggtc
     2161 aacgctgcgg gcagcaggcg accgtgatcg agcagcgtgt cgtcgagatc gaacaaaagg
     2221 ccccggagcc ctcgagctcg ctccgagctc agcgcgctgg ctggttgcat caggcgctcc
     2281 cggtcacttg ggcgatgacc gtgctcaagt agagcccctc cgggaatgcg gccggcactg
     2341 ggtgatccgg gccttggtag atccgttcga ggatggtcgc tcgaaccccg acgtcgcgtg
     2401 ctccaagagc gagcgagcgc gtgaggtcca tcattccgag cgcggcgctg cacgagctca
     2461 cgatcaaaag gccgttgggc tcggttgcgc gagcgcccgc cgcgcacagc cgccgcatcg
     2521 cctcgagcgc gcgttcgcgt cgggcgcggc tcggcgcaag cttcggcgga tcgacgatca
     2581 cgagatcggc cccgccctcg gccccgacgc gcttcaacgc ggccgatgcg tcgccgcatt
     2641 cgaaacgcac cttacccgag agcccatttc gagcggcgca ttctgcggcc acttccagag
     2701 cgaccgcgct cgtgtcgacg gcttcgaccg aaacggctcc accccgcgcc gccgaaagcg
     2761 caatcgcccc cgtgtacgaa tagagatcga gcacccggcg ccctcgcgcg aggctctcga
     2821 cgcgctcgcg cagcggccgc tggtcgaagt aaaatcccgt cttctgtccg agctcgagcg
     2881 gtacgcgaaa gcgcaggtcg cgttcggaaa actcgaaggc cgcgagtgac tcgtcgccac
     2941 ggaccacgcc ggtgccgggg cgaaaccctt cgctcgcggc ggtccgctct ggcgtccgat
     3001 cgacgatcgc tcggggcgag agagcgcgct ccaacaacga aagcacgatg ttctcgcgtt
     3061 gcttgagccc caccgtcccg aactgcagag acagcacgtc gcccagctga tcgacgacga
     3121 ggccgggaag atcgtcgccc tccgcgtgaa tcaagcgaaa gcccgtggtg cgatcgtcgg
     3181 gcaaaccgag cacgcggcgg cgctggatgg ccttcgaaat gcgctccgcg agcaggcctt
     3241 cgtcgatcga gcgattcgcg tcccgcgtgt agagccgcac ggggatggcg gagccagggg
     3301 aatacaggcc gcggccgaga ggattgccgc gggcatcccg gacaacgacc tcatccccgg
     3361 cacgtacacc gccctcgatt cgttcgacgg cttgcgcgaa cacccacggg tggccggccc
     3421 agacgggctg cacgtgaccg gcccgcaacg taacgtatcc gatactcacg attggcgggc
     3481 gagcgtagca tcaggaactc ggttcggagg cgcgcgatga cggcccgagc ccttcccggc
     3541 cttcccggat ccggcgaagg tgctaccctg ggccaagatt tcgggccttc acatgcgccg
     3601 catcgtcctc ttcgttggtt cggccgcagt ctcagcgctc ggcactgttt ggccatcgct
     3661 cgcgctcgcg cagacgtgcg cgcc
//
LOCUS       DASTKS010000532         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_530762, whole genome shotgun sequence.
ACCESSION   DASTKS010000532 DASTKS010000000
VERSION     DASTKS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530762"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFQ35_11035"
     CDS             complement(<1..904)
                     /locus_tag="VFQ35_11035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931216.1"
                     /translation="MGELLAGKYRIEGTLGVGGMGVVLAARNELIGQPVAIKFLVVND
                     EEYLGEATQRLLREARAAAQLRGEHVVRIYDVGELPNGTPFIVMERLHGADLGDISQN
                     IGPMPIPLAVGLIVQAARAISEAHAVGIVHRDLKPSNLFICRQPDGKALLKVVDFGIS
                     KSLQRDAVEMKTLTGARVALGSPHYMSPEQVRDARRVDERSDIWALGVILYELVAGRP
                     AFQADTYPGIFAAITADVPTALSTLRPEVAAGLEQVVQRCLEREPARRYQRAEELAQA
                     LDPYRAREIEFAELFPPLGNIENSP"
     gene            complement(1213..2739)
                     /locus_tag="VFQ35_11040"
     CDS             complement(1213..2739)
                     /locus_tag="VFQ35_11040"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET9931217.1"
                     /translation="MRWFSSPRFGSAESSYTLTRFVLLRGSGFIYSVAFSIVWFQFPA
                     LCGSKGLLPAADFLRWVAERLGSRGAGFARLPSIFWLSASDASLRGAAIAGLVLSIAA
                     LLGYSNAITQALLWALYLSFVHVGQIFYGYGWESLLCEAGFLAIFLAEPWRLHPFSPS
                     PPPTLVIVLFRWLTFRVMFGAGLIKLRGDACWRELSCLSYHYETQPNPGPLSIYFHRM
                     PDVFHRLGVLFNHAVELVAPFFVFGPRRARLVAGASIVTFQVVLILSGNLSFLNWLTL
                     IVALSCFDDGVFARLLPRALSKRAADIERVSVLGRSRRVVLVVLSIAVALLSVNPAVN
                     MLSPRQAMNASFDPLMLVNTYGAFGSVGRERLGAIIQGTNDIDPAPAAHWETYELPCQ
                     VGAPERRPCLVTPYHYRLDWQMWFVPLAGPVAAPEPWLVHLVARLLEADPLVLSLFQK
                     NPFPSSPPRFVRVALYRYRFSRPGEAGYWQREYLGNILPPLSRDDPELHRYLAERGWE
                     "
     gene            complement(2875..>3724)
                     /locus_tag="VFQ35_11045"
     CDS             complement(2875..>3724)
                     /locus_tag="VFQ35_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET9931218.1"
                     /translation="LVAERGRGAQVLLTNKAVIDAEALASLPDLRGICVLATGYNVVD
                     VAAARARRIPVSNVPSYSTPSVIEHTFALLFELCRAVGKHDDAVHAGEWVRALDFSFV
                     KSSQRELAGRTFGVVGYGEIGKGVARAASAFGLRVLATPSRRSPPEPFVEVRSTTELV
                     RECDILSLHCPLTAETEKMVNTELLSTMKPTSLLINTARGALVDEAALARALERGVIA
                     GAALDVLSTEPPAAENPLLTAPNCIITPHLAWTSIEARQRLVTETIGNVRGILTGNPR
                     NVVNGV"
BASE COUNT          758 a         1217 c         1183 g          566 t
ORIGIN      
        1 tcggtgagtt ctcgatgttg ccgagcggcg gaaagagctc ggcgaactcg atttcgcgag
       61 cgcggtacgg gtcgagcgct tgcgcgagct cttcggcccg ctgataccgc cgtgccggct
      121 cgcgctcgag gcagcgctgg acgacctgtt cgagtccagc cgcgacttcg ggccgtaacg
      181 tcgaaagcgc ggtcggaaca tcggccgtga tcgcggcgaa gatgcccggg taggtgtccg
      241 cttgaaatgc gggtcgtccg gcgacgagct cgtaaaggat cacgccgagc gcccagatgt
      301 cactgcgctc atcgactcgc cgagcgtcgc ggacctgctc tggcgacatg tagtgcggag
      361 acccaagcgc gacgcgtgct cccgtgagcg tcttcatttc gacggcatcg cgctggagcg
      421 acttcgagat gccgaagtcc acgaccttca atagcgcctt gccgtcgggc tgccgacaaa
      481 tgaagagatt cgacggcttc aggtctcggt ggacgatgcc gaccgcatgc gcctcgctga
      541 tcgcgcgagc ggcctgcacg atgagaccga ccgccaacgg aatcggcatg gggccgatgt
      601 tctgcgagat gtcgcccaag tcggcgccgt gaaggcgctc catcacgatg aaaggagtgc
      661 cgttcggcaa ctcgccgacg tcgtagatgc gcacgacgtg ctcaccgcga agttgtgccg
      721 ccgctcgggc ctcccggagc agccgctgcg ttgcttcgcc gaggtactcc tcgtcgttca
      781 ccaccaaaaa cttgatcgca acgggctgtc caatcagctc gttgcgcgcg gccagaacga
      841 cacccattcc gcctacgccg agcgtcccct cgatccggta tttcccagcg agaagctcgc
      901 ccaaccgcgg aacctcgcta ccgggcggaa tgagttgaga cgaattcgct gacggctcgg
      961 gcacaggtat ccttttcggt atatcgcgag atacgcgcct cgtcatccga acgcgagcat
     1021 ctcgtcgact tcgcccgcgg gtccggtccc aacgggcggc tcgaactgca acaatcaagc
     1081 acttccgaaa caccgcaaag aggcgccgtg taggcacccg tccgaggtgt ggcgagctga
     1141 gcgcaccgcc gggcgcgtca gttcgccctt ggccgcggct tccccggacg caccgccacg
     1201 acgccgcgtg cgttactccc agccccgctc cgcgaggtat cgatgcagct ccggatcgtc
     1261 acgggaaagc ggcggcagga tgttcccgag gtactcgcgc tgccaatagc ccgcctcacc
     1321 tggtctgctg aaccgatacc gatatagcgc cactcgtacg aagcgtggtg gcgaggaagg
     1381 gaaagggttc ttctgaaaca acgacagaac gaggggatct gcctccaaaa ggcgcgccac
     1441 caagtgaacg agccaaggct cgggggccgc gaccgggccg gctaagggaa cgaaccacat
     1501 ctgccagtcc agtcgatagt gatacggggt cacgaggcac ggccgacgct cgggcgctcc
     1561 gacctgacac ggcaactcgt acgtttccca atgcgctgcg ggagcggggt cgatgtcgtt
     1621 cgttccttga atgatggcgc cgagtcgctc gcgcccaacg ctgccgaatg cgccgtacgt
     1681 gttcaccagc atcagagggt cgaaggaagc gttcatcgcc tggcgcggcg agagcatgtt
     1741 caccgccggg ttcacgctga ggagcgccac cgcgatcgaa agcacgacca gcacgactcg
     1801 gcgggaacgc cccaaaaccg aaacgcgttc gatgtccgcc gcgcgcttgc tcagcgcacg
     1861 cggaagtagc cgggcgaaga ctccatcgtc gaagcacgac agcgcgacga tcaacgtgag
     1921 ccaattcaag aaggagaggt tcccgctcag aatcagcacg acttggaagg ttacgatcga
     1981 agcgccggct accagacggg cccgccgcgg accgaagacg aagaacggcg cgacgagttc
     2041 gacggcgtgg ttgaacaaca cgccgagccg atgaaaaacg tccggcatgc gatgaaagta
     2101 gatcgatagc gggcctgggt tcggctgcgt ctcgtagtgg tacgagagac acgaaagctc
     2161 gcgccagcag gcgtcgcctc gcagcttgat gagccctgcg ccgaacatca cgcggaacgt
     2221 gagccagcgg aacaagacga tgaccaacgt cggaggcggg ctcggcgaaa aggggtgcag
     2281 cctccagggc tcggccagga aaatcgccag gaagcccgct tcgcaaagca gagactccca
     2341 gccgtacccg taaaagatct gccccacgtg cacgaaagag agatagagcg cccagagcaa
     2401 cgcttgcgtg atcgcgttcg agtagccgag cagcgccgcg atcgagagta cgaggcctgc
     2461 gatcgccgcg ccgcgcaagc tcgcatccga cgcgctcagc cagaagatac tgggcaaccg
     2521 cgcgaagccg gcgccgcggg agcccaggcg ctcggccacc cagcgcagga aatccgcagc
     2581 cggaaggaga cccttgctgc cgcaaagcgc tggaaactga aaccagacga tcgaaaatgc
     2641 gacggagtaa atgaaaccgc tgccccgtag cagaacgaaa cgagtcagcg tgtagctcga
     2701 ctccgcgcta ccgaagcgcg gcgaagagaa ccatctcacc gattcggcaa tcgaatgctc
     2761 ccgcgccgca tcggcgttcg ccgtgcccgc ggggctcgcc tccgcttcgc ctggagaggt
     2821 cacatgaacc agcataacgc gcgccgggca acgccgctgg aagacggagg ccgctcaaac
     2881 gccgttgacg acgttgcgtg gatttccggt caggattccc ctgacgttgc cgatcgtctc
     2941 cgtcacgagg cgctgccgcg cttcgatgct cgtccatgcc aagtgcggag taatgatgca
     3001 gttgggcgca gtgagcagag ggttctccgc cgccggcggc tcggtcgaga ggacgtccag
     3061 cgcggcaccg gcgatgacgc ctcgctcgag ggcccgcgcc aaagcagcct cgtcgaccaa
     3121 agcgccccgc gcggtattga tgagcagcga cgtcggcttc atcgtgctca agagctcggt
     3181 gttcaccatc ttctccgtct ccgcggtgag cggacagtgc agcgacaaga tgtcgcattc
     3241 gcgtacgagc tcggtggtgc tgcgcacctc gacgaaaggc tccggcgggc tacggcgtga
     3301 cggcgtcgcg agcaccctga gcccgaacgc gctcgcagcg cgcgctacgc ccttgccaat
     3361 ctcgccatat ccgaccactc cgaaagttcg tccggcgagc tcacgttgag aggacttgac
     3421 gaagctgaag tccagagcac ggacccactc ccccgcatgc accgcgtcgt cgtgcttacc
     3481 cacagcgcgg cagagctcga acaaaagcgc gaacgtgtgc tcgatgacgc tcggcgtact
     3541 gtaagaaggc acgttcgaaa ctggtatcct ccgagcgcgg gctgccgcga cgtcgaccac
     3601 gttgtaaccg gtcgcgagca cacaaatgcc ccgcaagtct ggcaaggacg cgagcgcctc
     3661 cgcgtcgatg accgccttgt tcgtcagcaa tacttgcgca ccgcgcccac gctccgcaac
     3721 gagc
//
LOCUS       DASTKS010000533         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_84474,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000533 DASTKS010000000
VERSION     DASTKS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84474"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..402)
                     /locus_tag="VFQ35_11050"
     CDS             complement(76..402)
                     /locus_tag="VFQ35_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9931219.1"
                     /translation="MTYEYLCTACGHAWEAEQSISEAPLKECPTCHAEAAKRQVSGGA
                     GFILKGGGWYADLYSSSKSEKKAESAESKTSSSSDSASDAKKSSDSTASTASGSTSKA
                     TPAAAS"
     gene            806..>2439
                     /locus_tag="VFQ35_11055"
     CDS             806..>2439
                     /locus_tag="VFQ35_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9931220.1"
                     /translation="MHIKKLEIAGFKSFVDRTVIHFDHDVIGIVGPNGCGKSNIVDAI
                     RWCLGEQSAKHLRGRAMEDVIFNGSESRNQAGMAEVTITFDNTNPEYAASLPIEYKDY
                     PEIAVTRRLFRDGTSEYLVNKTQVRLRDITELFLGTGVGTKAYSIVEQGRIGQIVSAR
                     PEDRRLFIEEAAGITKYKQRRKMAERKMDLTRQNLLRINDIVAEIDRNRASLKRQVAK
                     AERFIEYRRELDDLVLHDPSHRLLEMIVMDRVERGAYEQAAAATTGLRDQLRGAETVI
                     DTSRAEAAGIEAQADSASQSAFEADNEVTTLHAEIERARDRLTHLGQRLETGQVELEE
                     LGMRRSGLSSELESLLSRVSELSSEERAREADALAETESLSGLQAEETRANEEVLALR
                     KQSGGLSAQAAALEARVDGVLQRVADARARRDRLTQEQEGLEGERAAGLARQSALETS
                     VAELAEGKRFTEAERESLRVEMESLRGRQLESERAVDSIKNELGIRRNRLRALEDLHR
                     RLEGVGAGARALLSKNDARVLGLVADRIQVPEEVTLA"
BASE COUNT          471 a          777 c          766 g          425 t
ORIGIN      
        1 cgccaatctc gccgtcgagc cgcgcgatgc gttcccgaat ttcctgaacc tcgcgccgcc
       61 gatctgccat gcaccttagg atgctgcggc aggcgtcgct ttgctagtag aaccgctcgc
      121 cgtcgaagca gtgctgtccg aggatttctt ggcgtctgaa gcgctgtccg acgacgagga
      181 cgtcttcgat tcagcgctct cagctttttt ttcggatttc gacgagctgt acagatctgc
      241 gtaccagccg ccccccttga gaatgaagcc agcgccgccg gacacctgac gcttcgctgc
      301 ctcggcgtgg caggttgggc attccttcag gggtgcttcg ctgatggatt gctctgcttc
      361 ccaagcatgt ccacaggcag tgcacaggta ctcataagtc atcgcttcga aaccttcttg
      421 ctcgatacgc tggccggaaa acctcggcgt ttcgggtact ccgggacact agtgagtcgc
      481 gctcggctct ttgtcaacat cgcctcgccg cgtacgcgac tttcgtggct actcagctga
      541 cgagagagag caaacgggct cgtttttggt caaaatgaga cgaccggccc gtcaggacac
      601 gacgtttccc gaccaattcg tgcgatgaac gcagctcgct gtacgtttgc gcttgtgtgc
      661 atccgttcag gcgcgtagaa agaactctct gagacagcgc gtgacgatac atgtcatgcc
      721 acccgcctgg tcaggggtct cgattggccg gccgccgtgg gatgcgctgg ctgctataac
      781 cgctgagctg caggggtagt tatcgatgca tatcaagaag ctcgagatcg caggcttcaa
      841 gtccttcgtg gaccggactg tgatccactt cgaccacgac gtcatcggca tcgttggccc
      901 gaacggctgc ggcaagtcga acatcgtgga tgcaattcgg tggtgtttgg gcgagcagag
      961 cgccaagcac ctgcgcggcc gcgcgatgga agacgtgatc ttcaacggct ccgagtcgcg
     1021 caatcaggcg ggcatggcgg aggtgaccat caccttcgac aacacgaacc ccgagtacgc
     1081 cgcaagcctg ccgatcgaat acaaggacta cccggaaatc gccgttacgc gtcgcttgtt
     1141 ccgggatggg acgagcgagt acctggtgaa caaaacccag gtccgtttgc gtgacatcac
     1201 cgagctcttt ttgggcacgg gcgtcggtac caaggcgtac tcgatcgtcg agcagggccg
     1261 catcggccaa atcgtcagcg cccgcccaga agatcggcgc ctcttcatcg aggaggcggc
     1321 cggcatcacc aagtacaagc agcgccggaa aatggccgag cgcaagatgg atttgacgcg
     1381 gcagaacctg ctgcgaatca acgacatcgt tgccgaaatc gaccgcaatc gcgcatcgct
     1441 caagcgccag gtcgcgaagg ccgagcgctt catcgagtat cgccgcgagc tcgacgatct
     1501 ggtgctccac gacccatcgc atcgtctgct cgagatgatc gtgatggacc gggtcgagcg
     1561 cggcgcttac gaacaagccg cagcggccac gactggcttg cgcgaccagc tgcgcggagc
     1621 cgagacggtc atcgacacct ctcgggccga agccgccggg atcgaagctc aagcagactc
     1681 ggcgtcgcag tccgccttcg aggccgacaa cgaggtcacg accctacacg ccgaaatcga
     1741 gcgcgcacgc gaccgtctca cccacctcgg ccagcgtctc gaaacgggac aggtcgagct
     1801 cgaagagctc gggatgcggc gctctggcct cagctccgag ctcgaatcgc tactttcgcg
     1861 cgtgagcgag ctgtcgagcg aagagcgcgc acgcgaagcc gatgccctcg ccgagaccga
     1921 atcgctgagt ggcctccaag cggaggaaac gcgcgcgaac gaagaggtgc tcgccctgcg
     1981 aaagcaatcg ggcggactca gcgcccaagc cgccgccttg gaagcgcggg tcgatggcgt
     2041 gctccagcgc gtcgccgacg cgcgtgctcg ccgcgaccgc ctgactcaag agcaagaagg
     2101 tttggagggc gaacgcgccg cgggactcgc tcgtcagagc gccctcgaga cgagcgtcgc
     2161 cgaactcgcg gaaggcaagc gcttcaccga ggccgagcgc gagtcgctgc gcgtcgagat
     2221 ggagtcgctg cgcggccgcc agctcgaaag cgagcgcgcg gtcgactcga tcaagaacga
     2281 gctcggcatc cgtcgcaatc gccttcgcgc cctcgaagat ctgcatcgcc ggttggaggg
     2341 cgtgggcgct ggcgcgcgcg cgctgctctc gaagaacgac gcccgcgtgc tcgggctcgt
     2401 cgccgatcgc atccaggttc cggaagaggt cacgctcgc
//
LOCUS       DASTKS010000534         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_307307, whole genome shotgun sequence.
ACCESSION   DASTKS010000534 DASTKS010000000
VERSION     DASTKS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307307"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ35_11060"
     CDS             <1..353
                     /locus_tag="VFQ35_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931221.1"
                     /translation="LGVPLLALACQNDLDHPPPVNGSDAGAPDDTPGVVAGPGAGPSS
                     GTAQGGNSSVSGGGVVSGVFTGGSTGLGGTLGTGGTIGAGNAAGFGVTTGGTVNSFGF
                     GGNPITPGTSGGFP"
     gene            complement(358..>3189)
                     /locus_tag="VFQ35_11065"
     CDS             complement(358..>3189)
                     /locus_tag="VFQ35_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931222.1"
                     /translation="QRPFAAENVAQTLHSITFSAPPDLEALRPDLPLALVELVYGTLA
                     KDRNQRVPSLRQVSAVLDEVLRGGSDASPRDAAESAKRRAAAPLPLEELSERARAFPN
                     NVPPLGSTLVGRKAELSELSLRLSEPGTRLLSITAPGGMGKTRLALEFAQELVIVGQR
                     RGFDARNAWFEDGVFWVQLTALASSESIVPAIAEVLGVSSWPGQNLEEQLAAFLREKR
                     LLLVLDNFEHLLDGASRVGELLAKAPGVTMLVTSRERLGLSVETVFPLAGLEFPLASE
                     RGQPDDFSAVRLFVQCARQQRPGFVLDPQSARDAARICRLVEGIPLGIVLSASWAGTL
                     SPAEIAEELGNGLDFLATEWRDVPERQRSMRVVFEQSWSRLSEAERDVLSSLAVFRGG
                     FTREAAVRVAGANLRLLSSLIDKCLLRRVPSNGRFETHELLRQYAREKLTEQPARHAE
                     CLDRHAEFFFRFVTSREAALKSPSPALASSELEAELDNVRAAWSYLVESCRLPELAEM
                     AEPLHVFYARRAAFSEGEAAFAALSASLRKTTELPPESRRLLAYASSLRAMFLRAQGR
                     YQQACELLSEALGLLDAEQQPRETAFALATHGSALAMIGQLELARDLAEKAAESYRTT
                     SDAWGLANALESLGRLHANAGDLLRAASAYRESIEVQRRAGLLESGTMGLAIALVQQG
                     NYVEGRASMLQALATFETAGDRWNAMRCRMHLANTQRNLGEYESAEALARQCLDFCVE
                     VGNRDHEVWALFQLGNIMKEQARYAEAEAQFRLAHEKSARSGEVGKVALAQLEFGDLA
                     LIRGDFALAKESLAQSLAGFERAGQGWGKALALDVSALVAVNEGDLATAENLFSRALS
                     LSLSLGLLPFACNVVAGIASLRSKQGSLVRAAELVGLARSHRAVERHTLTRRVNPLLA
                     ALALSLGAEEFEAALARGASASLERALES"
BASE COUNT          548 a         1087 c         1080 g          474 t
ORIGIN      
        1 acctcggggt tcctctcctc gcgctggcct gccaaaatga tctcgaccac ccgccgcccg
       61 tgaacggtag cgacgccgga gcgcccgacg acacgccggg agtcgtcgcc ggaccaggcg
      121 cgggaccgag ctcgggaacg gcccaaggag gcaacagctc ggtgtctggc ggcggcgtgg
      181 tttcgggcgt cttcactgga ggctcgacgg gcctcggcgg cacgttgggc accggcggta
      241 caatcggcgc gggcaacgcg gctgggttcg gtgtcaccac gggtggaacc gtcaactcct
      301 ttggatttgg cggaaacccg atcacgcccg gcacgagcgg cggctttccg taggcgctca
      361 gctctcgaga gcgcgctcga gcgacgccga ggcaccgcgg gcgagcgccg cctcgaactc
      421 ttcggcgccc aaagacagtg ctagcgcagc caacagcggg ttcactcgcc gcgtcagggt
      481 gtgccgctcg acggcgcggt gactgcgcgc gagcccgacg agctcggcgg ctcgcaccaa
      541 cgagccctgc ttgctgcgga ggctcgcgat gccggccacc acgttgcagg caaagggcaa
      601 gagcccgagc gacaaggaaa gcgagagagc gcgcgagaac aaattttcgg ccgtggcgag
      661 gtcgccctcg ttcacggcaa cgagggcact cacgtcgagc gcgagcgcct tgccccagcc
      721 ctgacccgcg cgttcgaaac ccgcgaggct ctgcgcgaga gattccttcg cgagcgcgaa
      781 gtcgccgcgt atcagggcca gatcgccgaa ctcgagctgc gcgagggcga cttttcccac
      841 ctcgccggag cgtgcactct tctcgtgggc gagcctgaat tgggcctcgg cctcggcgta
      901 tcgcgcctgc tccttcatga tgttgccgag ctgaaacagc gcccacacct cgtgatcgcg
      961 gtttcccacc tcgacgcaga agtccaggca ttgccgcgcg agcgcctcgg ccgactcgta
     1021 ctcgccgagg ttccgttgag tattcgcgag gtgcatccgg cagcgcatcg cattccagcg
     1081 atcgcccgcg gtctcgaacg tcgcgagcgc ctgcaacatg gaagcgcgac cttcgacgta
     1141 gtttccctgc tgcacgagcg cgatcgcaag acccatggtg ccagactcga gcagccccgc
     1201 ccgccgttga acctcgatgc tctctcgata cgcggacgcc gcgcgcaaca gatcgccggc
     1261 attggcgtgc agacgaccga ggctctcgag cgcgttggcg agaccccagg cgtcgctcgt
     1321 ggtgcgataa gattcggcgg ctttctccgc gaggtcacgc gcgagctcga gctgcccaat
     1381 catcgcgaga gcgctgccgt gggtcgcgag ggcgaaagcg gtctctcggg gttgctgctc
     1441 ggcgtcgagc agcccgagcg cctcgctcaa gagctcgcag gcttgctggt agcgcccctg
     1501 ggcccggagg aacatggcgc gcagcgacga cgcatacgcc aaaaggcgcc ggctctcggg
     1561 cggtaactcg gtggttttgc gtaggctcgc cgacaacgcc gcgaacgccg cctctccctc
     1621 cgaaaacgcg gcccggcgcg cgtaaaacac gtgcagcggc tccgccatct cggcaagctc
     1681 gggtaagcgg caactctcga cgaggtagct ccacgcggcg cgcacgttgt cgagctctgc
     1741 ctcgagctcg ctcgaagcga gcgccgggct cgggctcttg agcgcagcct cgcgcgaggt
     1801 gacgaaacga aagaagaact ccgcgtgacg atcgaggcac tcggcgtggc gcgccggctg
     1861 ctcggtgagt ttctctcgtg cgtattgacg caagagctcg tgggtctcga agcgaccgtt
     1921 gctcggcacg cgccgcaaga ggcacttgtc gatgagcgac gaaaggagcc ggaggttggc
     1981 ccccgcgacg cgaaccgcgg cctcccgcgt gaagccaccg cgaaaaaccg cgagcgacga
     2041 gagcacgtcg cgctcggctt cgctgagccg cgaccacgac tgctcgaaca cgacgcgcat
     2101 gctgcgctgt cgctcgggga cgtcgcgcca ctcggtcgcc aagaagtcga gaccgttgcc
     2161 gagctcttcg gcgatctcgg ctgggctcag ggtgcccgcc cacgatgccg agagcacgat
     2221 tccgagcggg atcccctcta cgagacgaca gatgcgcgcc gcgtcgcggg ccgactgcgg
     2281 gtccagcacg aagccgggcc gctgttgccg cgcgcattgc acgaagagcc gtaccgcgct
     2341 gaagtcgtcg ggctgtcccc gctccgaagc cagcggaaac tcgaggcccg cgagcgggaa
     2401 caccgtctcc acgctgagcc caaggcgctc acgcgacgtg acgagcatgg taacgcccgg
     2461 agcctttgcg aggagttctc cgacgcggct cgccccgtcg aggagatgct cgaaattgtc
     2521 gagcacgagc aagagccgct tttcgcgcag gaacgccgcg agttgctctt ccaggttctg
     2581 ccccggccaa ctcgataccc cgagcacctc cgcgattgcg ggcacgatgg attcgctaga
     2641 agcgagcgcg gtcagctgaa cccagaagac gccgtcctcg aaccatgcgt tccgcgcgtc
     2701 gaagcctcgc cgctggccga cgatgacgag ctcctgggcg aactccagcg cgaggcgcgt
     2761 cttccccatg ccgcccggcg ccgtgatgct gagtagacgg gttccaggct cggataggcg
     2821 caaagacagc tccgagagct ctgccttacg accgacgagg gtcgagccga gcggaggaac
     2881 gttgttcggg aaggcgcgag cgcgctcgga cagctcctcg agcggcagcg gagctgcagc
     2941 gcgacgcttt gcgctctcgg cggcgtcgcg cggcgacgca tccgagccgc cgcgcaatac
     3001 ctcgtcgagc acggcgctca cttgccgcaa gctcggcacg cgctgattac gatcctttgc
     3061 gagggtcccg tacacgagct cgacgagcgc gagcgggaga tccggccgaa gcgcctcgag
     3121 gtccggcggc gcagaaaagg tgatgctatg aagcgtttgc gcgacatttt ccgcggcaaa
     3181 ggggcgctg
//
LOCUS       DASTKS010000535         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_307312, whole genome shotgun sequence.
ACCESSION   DASTKS010000535 DASTKS010000000
VERSION     DASTKS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307312"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFQ35_11070"
     CDS             complement(<1..1281)
                     /locus_tag="VFQ35_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HET9931223.1"
                     /translation="MDLEGLREKLSASSSLRPLGRVSGVTGLLVRLSVAGARVGDVVT
                     IRRRGEPLLAEVVGLAAGEALAVPLGELSGVGVDDEVENHGEALSIASGTGLLGRVLD
                     GLGRPLDGRPLPDGLRPMPVDRAPPSALTRRAVSEPLPTGVRVLDGLLTLGVGQRVGL
                     FAGSGVGKSTLLGAMARGTSADVVVVALVGERGREVGEFLRDSLGAEGLEKAVVVVAT
                     SDAPPVERLRAAQVATAIAEGFRDDGKRVLLLVDSVTRVARAQREIGLAVGEPPARRG
                     YPPSVFTLLPRLLERSGQGETGSITAVYTVLVEGGDMDEPIADEVRGVLDGHIVLSRD
                     LAARGIYPAVDVPVSISRVMDAVVSNEQALAARELRAALGAYAQRRDLVALGAYAKGS
                     DPKLDAALSIMPELERFLLQDPKQPEGFERTKSEV"
     gene            1345..2013
                     /locus_tag="VFQ35_11075"
     CDS             1345..2013
                     /locus_tag="VFQ35_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931224.1"
                     /translation="MMVLRLCCATLVLSLGCHGSMSAEAKMGQELDRQDPALQDPPEK
                     QGLASNSQPLSAEESARQAALAEPATNPDLALFGARHDFVVKDASGAVGCQCVSVLLG
                     PPSSGKLEWRGEMPRTKPETQLVVALVPGNCPDGAEGASYWGYRVDGNDVVVLLEGWK
                     PGRPRTLGAIVPRPAVGGQVYVAPVSSKLPYGKALAGGGARCAAGNPGPQRTTPLESS
                     APIP"
     gene            2250..2549
                     /locus_tag="VFQ35_11080"
     CDS             2250..2549
                     /locus_tag="VFQ35_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931225.1"
                     /translation="MGGAAEKRTTPSPHPTSALGRADYFHVGYVSLMVSCGEKAVLVV
                     TTSAEGTATNVARHRFELTCALAQGHAGAHRDAVHGEQWEAPPGQLKMLLRDENE"
     gene            complement(2553..3704)
                     /locus_tag="VFQ35_11085"
     CDS             complement(2553..3704)
                     /locus_tag="VFQ35_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9931226.1"
                     /translation="MSAFEFATAQQIVFGCGKLAELAVHACGLGSCGLLVTGSRPERA
                     DRARDLLEGAKINVVTLAVSKEPTTELARRGVELSREANVGFVIGMGGGSALDLAKAV
                     AALAANGGDPLDYLEVVGRGRALSKPSLPCILIPTTAGTGSEVTRNAVLQAEAERVKV
                     SLRSPHMLPKVALVDPELTLSVPRHVTAATGLDALTQVMEPYVSNARGALTDALCVEG
                     MRRGARSLLRAYEDGSDLGAREDLCVTSLFGGLSLANAKLGAVHGLAGPLGGAFEAPH
                     GALCARLLPNVMRANLRALEQRGHRELIERYRTIAGLLLGRPASPEEGIEWVEELVER
                     LGIPRLAAHGVTSAHFEDIVAKGRRASSMKGNPVELAAEELTRVLEAAL"
     gene            complement(3701..4027)
                     /locus_tag="VFQ35_11090"
     CDS             complement(3701..4027)
                     /locus_tag="VFQ35_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9931227.1"
                     /translation="MLIVHVEVQVKPEFVEDFKAATLQNARASLTEAGVLRFDVVQKE
                     DDPTRFVLVEAYRDDQAPLRHKETAHYAAWREAVESMMAAPRTSRRFRRVFPEEASWA
                     TVREGK"
     gene            complement(4036..4443)
                     /locus_tag="VFQ35_11095"
     CDS             complement(4036..4443)
                     /locus_tag="VFQ35_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931228.1"
                     /translation="MEVTQIVSNGFVSVSVDLVRQVVRVTRSSERGSIVQITRAFADA
                     VAALDGIGRARLGLLVDLRAAPGRNDPDFENAMAKQRVELLRGFAALAILVQTATGQL
                     QVSRIGREDGMDVAIFTDEFAALAWLEGARASG"
     gene            4629..>5203
                     /locus_tag="VFQ35_11100"
     CDS             4629..>5203
                     /locus_tag="VFQ35_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931229.1"
                     /translation="MRALGCLPLLLLGCAPPPSTRPLGGEEWVREERLADRKPHGSSE
                     PKLPEPVETEASAPAVAVTVAPQPIAPPPATVKPGDIEFKFEPLAKGTVVHVDTRVKL
                     EATFGSENAVAEGSERMDVRILEASAERVREVEVLYVKSDSSFRYVGGNEQSTKSGKR
                     YRVRFDTPPQVQLLDKGQGGDDDEDEDEDED"
BASE COUNT          918 a         1779 c         1697 g          809 t
ORIGIN      
        1 gacctcgctc ttggtgcgct cgaagccttc gggctgcttc gggtcttgca acaagaagcg
       61 ttcgagctcc ggcatgatgc tgagcgcagc gtcgagtttg gggtcgctgc ctttcgcgta
      121 agccccgagc gcgactagat cccggcgctg cgcgtaagcg ccgagcgccg ctcgaagctc
      181 gcgagccgcc agcgcttgct cgttcgatac gacggcgtcc atgactcgcg aaatcgagac
      241 gggcacgtcg acggcagggt agatgcctcg cgcggcgaga tcgcgggaca gcacgatgtg
      301 accatcgagc acgccacgca cttcatcggc gatgggctcg tccatgtcgc cgccctcgac
      361 caggaccgtg tacacggcgg tgatcgagcc ggtttcgccc tgcccgctgc gttcgagcag
      421 ccgcggtaag agcgtgaaca cgctcggcgg gtaaccacgg cgcgccggcg gctctcccac
      481 cgccaagcca atctcgcgct gagctcgagc gacgcgcgtc accgagtcga cgagcaagag
      541 cacgcgcttt ccgtcgtcgc gaaatccctc ggcgattgcg gtcgcgactt gagcggcgcg
      601 gagacgttcg actgggggcg cgtcgctcgt cgcgacgacg acgactgcct tctcgagccc
      661 ttccgcgccg agcgagtcgc gaaggaattc tccgacttca cgcccgcgct ctccgaccaa
      721 cgcgaccacg actacgtccg cagaggtgcc gcgcgccatc gcaccgagca gcgtgctctt
      781 gccgacgccg gaccctgcaa agagccccac acgttgaccg accccgagcg tgagcaaccc
      841 atcgagcaca cgcacacccg tcggcaacgg ctcgctgacg gcgcgacgag tcagcgccga
      901 cggcggcgcg cgatcgaccg gcatcgggcg caacccatcc ggcaacgggc gaccgtcgag
      961 aggacgcccg agcccgtcga ggactcgacc gaggagcccg gttccgctcg cgatggagag
     1021 ggcctcgccg tgattctcga cctcgtcgtc gacccccacg ccgctgagct caccgagcgg
     1081 caccgcgagc gcttcgcccg ctgcgagccc gaccacttcg gcgaggagag gctccccacg
     1141 gcgccgaatc gtaacgacgt cccccacccg agcaccggcg acgctcaggc gaacgagcag
     1201 ccccgtaacg ccgctgaccc gaccgagtgg gcggaggctg gacgacgccg aaagcttttc
     1261 ccggagcccc tcgagatcca atgtaaaatc gagactacaa cgaacatttg ccttctttcc
     1321 agcggaagtg cgaggctcgg gggcgtgatg gtcttacgcc tttgctgcgc gacgctcgtc
     1381 ctcagtctcg gctgtcatgg ctcgatgagc gccgaagcca agatgggaca ggagctcgac
     1441 cggcaagacc cggcgttaca agacccaccc gaaaaacaag ggctcgcgag taactctcaa
     1501 ccgctcagcg ccgaggagag cgcgcgacag gccgcgctgg ccgagccggc gacgaatccc
     1561 gacctcgcgc tgttcggggc gcgccacgat tttgtcgtca aagatgcgtc cggcgccgtg
     1621 ggctgccagt gcgtgtccgt gttgctcggg ccgccttcgt cgggcaagct cgagtggcgc
     1681 ggagaaatgc cgcggacgaa gccggaaacg caactcgtcg tcgcgctcgt accaggaaat
     1741 tgcccagatg gggccgaggg cgcgtcgtac tggggctacc gcgtcgatgg gaacgatgtc
     1801 gtggtcttgc tcgaagggtg gaagccggga cgccctcgga cgctcggcgc catcgttccg
     1861 cgcccggccg tcggcgggca ggtctacgtc gcgccggtga gcagcaagtt accgtacgga
     1921 aaagcgctcg ccggcggcgg agcgcgttgc gcggcgggga acccgggacc gcaacgaacg
     1981 acaccgctcg aatcgtccgc gccaatcccc taacgcggtt cgaacgcgtt tggaggtgct
     2041 ctgcgggaga gagcgaccca aggcggccga tgcagcgaca gccataaatt accggcgttt
     2101 tggcgaggag cgcgattgaa cgttcttgca aaaacgccct gctgcggcaa tttgcatcgt
     2161 cggccgttgg tcctggcggg catggacgag cgaacaaaag atttgggtac aaaagagact
     2221 gaaactccag gcctctgtgg tcgtagagta tgggtggcgc ggccgagaag cgcaccaccc
     2281 cctcccccca ccccaccagt gccctcggtc gcgccgatta cttccacgtc ggctatgtct
     2341 cgctgatggt ttcgtgtgga gagaaagcag tcctagtggt gacgaccagt gcggaaggca
     2401 ctgcgaccaa tgtcgcgcgt caccgtttcg agctcacgtg cgcgctcgcg caaggtcacg
     2461 cgggagctca tcgcgacgcg gtgcacggag agcagtggga agctccgcct ggtcagctca
     2521 agatgctcct gcgcgacgag aacgaatgaa tctcagagcg ccgcttcgag cactcgggtg
     2581 agctcttcgg cggcgagctc gacgggattg cccttcatgc tgctcgcgcg tcgtcccttc
     2641 gcgacgatgt cttcgaaatg cgcgctcgtc acgccgtgag cggcaaggcg tgggatcccg
     2701 agccgttcca cgagctcttc gacccactcg atgccctctt ccggagacgc ggggcgcccc
     2761 agcaaaaggc cggcaatcgt gcgataacgc tcgatgagct cgcgatgccc gcgctgctcg
     2821 agcgcgcgta ggtttgcgcg catgacgtta ggtaacaacc gcgcgcacag agctccgtgc
     2881 ggcgcctcga aagcgccgcc gagcggacct gccaagccgt gcacggcgcc gagcttcgcg
     2941 ttggcgagcg aaagcccgcc gaacagactc gtaacgcaga gatcttctcg cgcgccgaga
     3001 tcgctcccgt cttcgtaagc gcggagcagc gatcgcgcgc cacggcgcat gccttcgacg
     3061 cacagcgcgt cggtgagcgc gccgcgcgcg ttcgagacgt acggctccat gacttgagtc
     3121 agcgcgtcga gcccggtcgc ggccgtcacg tggcgcggca ccgagagcgt gagctccgga
     3181 tcgacgagcg cgaccttcgg caacatgtgg gggctgcgca agctcacctt cacgcgctcg
     3241 gcttctgcct gcagcacggc gttgcgagtc acctccgaac ccgtgccggc cgtcgtcgga
     3301 atcaagatgc aaggaaggct cggcttcgaa agcgcgcggc cgcgacccac gacctccaag
     3361 tagtccagtg gatcgccgcc gttcgcggcc aacgcggcca ctgccttggc caagtcgagc
     3421 gcgctgccgc ctcccatgcc gatgacgaag ccaacgttcg cctcgcgcga cagctcgacg
     3481 ccgcgccggg cgagctccgt ggtcggttct ttcgagaccg cgagtgtcac gacattgatc
     3541 tttgcccctt cgagcaagtc gcgcgcgcgg tcggcccgct cggggcgcga accagtgacg
     3601 aggagcccac aggagccgag tccgcaggcg tgcacggcga gctcggcgag tttgccgcag
     3661 ccgaatacga tttgctgcgc cgtcgcgaac tcgaacgcgc tcacttgccc tcgcgcacgg
     3721 tcgcccagct cgcctcttca gggaacacgc gtcggaaacg cctgctggtg cgcggagctg
     3781 ccatcatcga ctcgaccgct tcccgccaag cggcgtagtg cgccgtctct ttgtggcgca
     3841 ggggcgcctg gtcgtcacga tacgcttcga cgagcacgaa gcgcgtcggg tcgtcttctt
     3901 tttggacgac gtcgaaccga agcacaccgg cctcggtgag gctcgcgcgg gcgttttgca
     3961 gagtggcggc tttgaagtcc tccacgaact cgggtttgac ttggacctcg acgtgcacga
     4021 tgagcatgtt cgcttttacc cggaagcgcg ggcgccttct agccaggcaa gcgccgcgaa
     4081 ctcgtcggtg aagatcgcca cgtccattcc atcttcgcga ccaatgcgag agacctgaag
     4141 ctgcccagtc gccgtttgga ccaggatggc gagggcggcg aaccctcgca agagctcgac
     4201 ccgctgcttt gccatcgcat tctcgaaatc gggatcgttc cttcccggcg ccgcccgcag
     4261 atcgacgagc aaccccaggc gcgcccgccc gatgccgtcc agcgccgcca cggcgtcggc
     4321 gaaggcgcgg gtgatctgca cgatgctgcc gcgctcggag ctccgcgtca cccgcactac
     4381 ctgacgcact aggtcgaccg acacgctcac gaagccgttc gagacgatct gggtcacttc
     4441 cattatttac agggttcggc tcgaggcctt ccagccccga gtcggtggct cgacgtgtgg
     4501 gccaatgtgt gttgccccag cgctcgtacc gcaggctgcc ccaacgcttc ggaggaagtg
     4561 gggcacgggt tcgcttcatc gccccggtct gcctggtgca tgcggccctt tgcaacgata
     4621 ctgcccgtat gcgcgctctc ggctgcctcc ccctcctctt gctcgggtgc gcgccgccgc
     4681 ccagcacacg ccctctgggc ggcgaggaat gggtacgaga ggaacggctc gcggatcgca
     4741 agccccacgg ctcgtccgag ccgaagctac ccgaacccgt cgagaccgaa gcaagcgccc
     4801 cagccgtcgc cgtcacggtg gcgccccagc cgatcgcgcc cccaccggcc acggtcaagc
     4861 cgggcgacat cgagttcaag ttcgagccgc tcgccaaagg caccgtcgtc cacgtcgaca
     4921 cgcgagtgaa gctcgaagcg acattcggct ccgagaacgc ggtcgccgag ggcagcgaac
     4981 gaatggacgt tcgcatcctc gaagcgagtg ccgagcgcgt gcgcgaagtc gaagtgctgt
     5041 acgtaaaatc cgacagctcg ttccgctacg tcggcgggaa cgagcagagc accaagagcg
     5101 gcaagcgcta ccgagtgcgc ttcgacacgc cgccgcaagt gcagctgctc gacaagggcc
     5161 agggcggtga cgacgatgaa gacgaggatg aggacgagga cga
//
LOCUS       DASTKS010000536         4697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_84917,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000536 DASTKS010000000
VERSION     DASTKS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84917"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..647
                     /locus_tag="VFQ35_11105"
     CDS             132..647
                     /locus_tag="VFQ35_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931230.1"
                     /translation="MNQKTEAIGSVVVLEEGAEFPKWVAEYQRHATNSIVVAYSPGET
                     SADFAARFARRLAETRGELRVGILACGPRVDAEQLKVRESMARALMQAIEERGRGEIL
                     LAARVEASEDAKYAIFELAGVLCEGLHDGRCVVRVRFSSGRPESGIMPSVGGPFDSGD
                     MGYGAVGTGRG"
     gene            944..1474
                     /locus_tag="VFQ35_11110"
     CDS             944..1474
                     /locus_tag="VFQ35_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931231.1"
                     /translation="MSCDIARSREGDRCSLDEEGSASCADASSLVACHGGHFVQTPCR
                     GAKGCALEGDRAVCDTTLAIFGDVCKDEGKKACAIDKSAVLACKSMRMASLYACRGSR
                     GCTLANGKLDCDMSVASAGDSCDARQEGHVACSLDASSTLVCRAGSFVADEKCKPKTR
                     CEATEHETRCVPEKKI"
     gene            complement(1471..2493)
                     /locus_tag="VFQ35_11115"
     CDS             complement(1471..2493)
                     /locus_tag="VFQ35_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9931232.1"
                     /translation="MMRALSVEKFGLENLAVREVPRPRPGPGEALVRVRAASLNYRDY
                     LVAIGKYNPRLPRPLTLGSDAVGDIVEFGDAASAIGLSLGDRVVVHMVQGWLDGPPSR
                     NATQHTLGGPLPGVFSDYVITRADSLLRAPAYLDDAEAACLPCAALTAMSALAQSELL
                     ATGATLLTQGSGGVSLFTLQLGKARGVRVLATSRSTTKRARLLALGADEVLLADAPGW
                     GRSARMLSGGEGVEHVVEVTGGSTLAESLQAVRPGGTVSLIGTLGGSAAELDLLPIVM
                     RNVRVQGVFVGHLRRLRELLNECERTRLRPVIDSVYDLAEGPKAFERLASGEQFGKIC
                     LKIRVS"
     gene            complement(2549..>4697)
                     /locus_tag="VFQ35_11120"
     CDS             complement(2549..>4697)
                     /locus_tag="VFQ35_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931233.1"
                     /translation="GEVDATAGPAFVEFWGSPGAVPEGRNGLCVAAHFAPPIGSKFRV
                     VRSPRLGDVLSQLVAWVADRLPSKTRFEAASALEFLQKRWSSGMLATLGEALGWVGLL
                     DEYGARELQARSPARIAQRFVEDRVQRILDASAPFAGWVRRSIYAALLGMAERALVDS
                     EFPLAEPRRFDEWLSLVPSELERNVDLEWMRLSLTQIDSAIRPADVERAARRLPPGAF
                     RVITSLVEARLLRRDSQGNLRLEPAWLRGELERSAQASVLSRSPFDWGEALLRPHAAR
                     GLCEALLERTLREGGSAIESVLELEAEDQPGYAAAVDLSFRIAGIGRLLGAELSQELL
                     EGLWRESAQLVLEDPSGLPLPRLEFALEASRGGDGAEIAESLLSNGGYYLAALSISEQ
                     LDVRVSAPLRVLDPWHATKPEPSLGAVYDRIAESLGARPRWWRAAIQLVSRVRAVVGN
                     AVDAEAPHDLELPAQILDEVEHGVLSFSTLEHADEDRWLLPLLDLADEHGTRRVEVAR
                     AIWQAWDQAGRPDGAAFLAPTATEHLLFWGTIPAELLAALLVDVRRHNVPYAAFGDEQ
                     WEGFAEALRRNPELVSESAAWERMPSEWVSRLLALPLDWASTPESVKVLWRRFPELLS
                     NAVRRQLGPGTTNEDPSALAALLQAAPNDLASTLLGELLPRVYGIRPRARHAIRSLLR
                     RLVSERDAGWREAYATLSRLEREWRTLDAASAH"
BASE COUNT          842 a         1557 c         1591 g          707 t
ORIGIN      
        1 ctcgcgacgc caacggaaag agtctctgac tcacggcgca cgcgttgaac cacttcgctt
       61 cgccgaatag ctagaggcat cgggtcacac agaaggaggc gctcgacgtc gaactcgagc
      121 gtagggacgt gatgaatcag aagaccgaag ccatcgggtc cgtcgtcgtt ctcgaagagg
      181 gagcagagtt cccgaagtgg gtcgctgaat accagcggca cgctacgaac tcgatcgtcg
      241 tcgcctactc gcccggagag accagcgccg acttcgccgc gcgctttgcg cgacgcctcg
      301 ccgagacgcg aggggagctc cgcgtcggga tcctggcgtg tggtccgcgc gtcgacgcgg
      361 agcagctcaa ggtgcgcgag agcatggcgc gcgcgctgat gcaagccatc gaagagcgag
      421 ggaggggaga gatcctcctc gccgctcgtg tcgaagcgag cgaggacgcg aagtacgcaa
      481 tcttcgagct cgcgggcgtc ctgtgcgagg ggctccacga cggtcgctgc gtcgtgcgag
      541 tgcgcttctc gagtggccgt cccgagagcg gcatcatgcc gagcgtcggt ggacctttcg
      601 attcgggtga catgggctac ggcgcggtgg gtacgggccg gggctgagcg ttccggtcgg
      661 tcgttgaaaa gcgccgcgcg ggcgcgtata tggcggccac cgacgcgaaa tgagcaaagc
      721 tacacggcca ccgacgaggc ggcgcggggc gaggacgtcg ccgctggctg ctatcctggc
      781 tctgctcctc gcggcgcttg cgtgtcgtcc aggcgtgggc gggcgttgcg aaaaaggaga
      841 ggcccgatgc ctcgactcct cccgcgcgct cgcgtgtgag cgaggcgcct tcatcgaggt
      901 tccttgtcga ggcccgaagg gttgcgtcgc aaccgagagc gccgtgagct gcgacatcgc
      961 gcgcagccgc gagggagatc gctgctcgct cgatgaggaa ggcagcgcga gctgtgccga
     1021 cgcgagcagt ctggtcgcat gccacggtgg tcacttcgtc caaacgccat gccgcggcgc
     1081 caaaggttgt gcgctcgaag gcgatcgagc cgtgtgcgat accaccctcg cgatctttgg
     1141 tgacgtttgc aaagacgaag ggaagaaggc gtgcgcgatc gacaagagcg ccgtgctcgc
     1201 gtgtaagtcg atgcgcatgg caagcctcta tgcttgccgt ggctcgcggg gttgcacgct
     1261 ggcgaatggc aagctcgact gcgacatgag cgtcgcgagc gccggagact cctgcgacgc
     1321 gcgccaggag ggtcacgtcg cttgttcgct cgatgcatcg agcacactcg tttgtcgcgc
     1381 tggctcgttc gtcgccgacg aaaagtgcaa gcccaagact cgttgcgaag cgaccgagca
     1441 cgagacgcgc tgcgtccccg aaaagaagat ctagctcacc cggattttca ggcagatctt
     1501 accgaactgt tcgcccgagg cgagccgttc gaacgctttt gggccttcgg cgagatcgta
     1561 gaccgaatcg atgacaggcc gaaggcgagt gcgctcgcac tcgttcagga gctcgcggag
     1621 gcgtcgcaaa tggccgacga acactccttg aacgcgcaca tttcgcatca cgatcgggag
     1681 caggtcgagc tctgcggcac ttccgcccaa ggtgccgatg aggctgacgg tgccgccggg
     1741 acgcacagcc tggagggatt cggcgagcgt cgagcctccc gttacttcca cgacgtgctc
     1801 cacgccttct ccgccggaca gcattcgcgc gctcctgccc cagccgggag cgtcagcgag
     1861 gagtacctcg tcggcgccga gcgcgagtag ccgcgcgcgc ttcgtggtgc tgcgcgaagt
     1921 ggcaagcacg cgcacgccgc gcgccttgcc cagttgcagc gtgaacaacg aaaccccgcc
     1981 cgagccttgg gtgagcaggg tggcgccggt cgcgagcagc tcgctctgcg cgagagcgct
     2041 catcgcggtg agggcggcgc aaggcaaaca cgcagcttcg gcgtcgtcga ggtaggcggg
     2101 cgcccgaagc agactatccg cgcgcgtgat gacgtaatcg ctgaacactc ccggcagggg
     2161 gccgccgagc gtgtgttgag tggcgttgcg cgatggggga ccgtcgagcc agccctgcac
     2221 catgtgaacg acgacgcgat cgccgagcga cagaccaatc gcggacgcag cgtctccgaa
     2281 ctccacgatg tcgccgacgg cgtccgagcc gagcgtgagc ggccgcggca agcgcggatt
     2341 gtacttgcca atcgcaacca ggtagtcgcg atagttgagc gaagcggcgc gcacccgcac
     2401 gagcgcctct cccggccccg gtcgaggtcg aggaacctcc cgaaccgcga ggttctcgag
     2461 gccgaacttt tccacgctaa gagcccgcat cattggcaga ggctaaatca gcgttgtcgc
     2521 gctcgccaag cggtacgctc ggggaggctc aatgggcgct cgccgcgtcc agggtgcgcc
     2581 actcacgctc gagtcgcgac agcgtcgcat acgcttcgcg ccaaccggcg tcgcgctcgc
     2641 tgaccagacg gcgtagaagt gatcggatcg cgtgccgcgc ccgtgggcga atcccgtaaa
     2701 cgcgcggcaa aagctcgccg agcagcgtcg acgcgaggtc gttcggcgcc gcctggagca
     2761 gcgccgccaa ggcgctcgga tcttcgttcg tcgtgccggg gccgagctgt cgccgcacgg
     2821 cgttgctgag aagctcgggg aagcgccgcc acaacacctt cacggactcg ggcgtgctcg
     2881 cccagtcgag cggtagcgcc agcaagcgac tcacccattc gctcggcata cgctcccatg
     2941 ccgcgctctc gctgacgagc tcagggtttc gacgcaacgc ttcggcaaag ccttcccact
     3001 gctcgtcgcc gaacgcggcg tagggcacgt tgtggcgtcg cacgtcgacg agcagcgccg
     3061 ccaacagctc tgcagggatc gtcccccaaa acaaaagatg ctcagtcgcc gtgggcgcga
     3121 ggaaagccgc tccatcgggg cgccccgcct ggtcccacgc ttgccaaatg gcgcgagcga
     3181 cctcgacccg tcgcgtcccg tgctcgtccg ccaaatccag gagaggcagc agccagcggt
     3241 cttcgtctgc gtgctcgagg gtcgagaacg aaagcactcc gtgctccacc tcgtccaaga
     3301 tttgtgccgg tagctcgagg tcgtgaggcg cttcggcgtc gaccgcgttc ccgacgacgg
     3361 cccgaacgcg ggagacgagc tgaatcgccg cgcgccacca gcgcggacgc gccccgagcg
     3421 attctgcgat gcgatcatag acagcaccga gcgacggctc cggcttggtt gcgtgccatg
     3481 gatcgaggac acgcagcggc gcgctgactc ggacatcgag ctgctcactg atgctgagcg
     3541 cggccaggta atagccgcca ttgctcaaga gtgactctgc aatttcagct ccgtcgcccc
     3601 cgcgcgaggc ctccagcgcg aattcgaggc gcggcaaggg aagccccgac ggatcctcga
     3661 ggacgagctg agcgctctct ctccacaggc cttcgagcag ctcttggctg agctctgcgc
     3721 cgagcaagcg gccgatgccg gcgatccgaa aggagagatc gacggcagct gcataccctg
     3781 gctgatcttc cgcttccaac tccagcacgc tctcgatcgc tgagccgcct tcgcgcaggg
     3841 tacgctcgag cagcgcctca cagaggccgc gcgctgcgtg tgggcgcagc agcgcttcgc
     3901 cccaatcgaa aggagagcgc gacaggaccg aagcttgcgc gctgcgctcc aactcgcccc
     3961 gtagccacgc cggctcgagg cgcaggttgc cctggctgtc gcggcgcagc agccgcgctt
     4021 cgacgagaga agtgattacc cggaacgccc ctgggggcag acgccgcgcc gcacgctcga
     4081 cgtcggcggg ccggatcgcg ctgtcgattt gggtcaagga caggcgcatc cactccaagt
     4141 ccacgttgcg ctcgagctcg ctcggaacga gggaaagcca ttcatcgaag cgccgtggct
     4201 ctgccagcgg aaactccgaa tcgaccagag cgcgctcagc catgccgagc agtgccgcgt
     4261 agatcgagcg ccgaacccaa cccgcgaacg gcgcgctcgc atcgaggatg cgctgaacgc
     4321 ggtcctcgac gaaacgctgc gcaatgcgag ccggtgagcg cgcctggagc tcgcgtgcgc
     4381 cgtactcgtc caggaggccg acccagccca gcgcctctcc gagggtggcg agcatccccg
     4441 agctccagcg cttctgcagg aactccaagg cagacgccgc ttcgaaccgg gttttgctcg
     4501 gcaagcggtc ggcgacccag gcaacgagct gcgaaagcac gtccccaaga cgtggcgagc
     4561 ggacgacgcg gaacttggac ccgattggcg gtgcgaaatg ggcagcgacg cagagcccgt
     4621 ttcgaccctc cggaaccgcg ccgggcgagc cccagaactc aacgaaggct gggccggccg
     4681 tcgcatcgac ctcgccc
//
LOCUS       DASTKS010000537         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_307477, whole genome shotgun sequence.
ACCESSION   DASTKS010000537 DASTKS010000000
VERSION     DASTKS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307477"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..813
                     /gene="flgG"
                     /locus_tag="VFQ35_11125"
     CDS             28..813
                     /gene="flgG"
                     /locus_tag="VFQ35_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HET9931234.1"
                     /translation="MFRSLHIAATGMAAQETQLESISNNISNGNTVGYKKQRVDFQDL
                     LYQTIRAPGAPTGTTTTSPTGLQLGSGVRVVSTSRVFSQGTVLTTNNPLDVAIEGNGF
                     FVVQQNDGKPAYTRAGALRTDPQGRIVTPEGLPLDPPVTIPQGATNISIAANGKVSVT
                     LPGETNPADVGQIQIANFINPAGLQSIGHNLMTTSAASGDAQVGEPGTDGRGALLQGA
                     TEQSNVDIVEEMVGMISAQRAYEINSKVITTADDMLRAATQMR"
     gene            820..1563
                     /locus_tag="VFQ35_11130"
     CDS             820..1563
                     /locus_tag="VFQ35_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HET9931235.1"
                     /translation="MRPFLAALSLSAALLGLSPAALAEPTRTLESARIQLGQIAETTD
                     AELAAVDLGASPPPGSSRLISREDVMRELRAQGLEQRKLSFPPVLRVVSASRRYSPSE
                     LSALLLPEVNAALPPGVTLKELKAPRALVTAPHVTVGRIKLPKLARRAGPVDLTLVAD
                     LMHDGAVLTRVAFGLAVDVSEQAAAPLVDKGARVDLVILTGSARISASAVALEPADVG
                     DVVSFKVSTTQRVLRARLESRTQAQVVSQ"
     gene            1560..2258
                     /locus_tag="VFQ35_11135"
     CDS             1560..2258
                     /locus_tag="VFQ35_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HET9931236.1"
                     /translation="MTKSLAALLLLSTLLGACGPAHIAPFTPRERKFEAGEYAATKKG
                     SQPATGSIYSEAQPGFLEDTRALRVGDIVLIHINEHADAQGGATTNLKKETNREAGVD
                     SFLGLVPAIQKAYPNIDPTKLLAMASKSGFNGEGTTQRAGTLNGSIGVHVKQELPNGD
                     LYVEGTKVVMINHEEYHLYISGVLRPSDIAPDNSVDSSLIADARVEFTGRGDIDDQVN
                     RGWLTKVLDFINPF"
     gene            2262..>2804
                     /locus_tag="VFQ35_11140"
     CDS             2262..>2804
                     /locus_tag="VFQ35_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET9931237.1"
                     /translation="MRSRGTFTYSFARWLSLLAVLACIGFSPSARADRLRDLCDVVGA
                     RDNQLLGYGIVTGLNGTGDDLTAPFAMQSLRALLRRLGVQVDSGQMRLKNIAAVVVTA
                     TIPPFARNGSKLDITIASIGNAKSLRGGVLLQTPLHGADRKTYAVGQGPITIGGFSAG
                     SGGSSVQDGVTTTGRISNGAL"
BASE COUNT          504 a         1017 c          835 g          448 t
ORIGIN      
        1 cgttgcagtg ctgagtcacg gagccccatg tttcgatccc tacatatcgc cgcaaccggg
       61 atggctgctc aagagacgca gctcgagagc atctcgaaca acatctcgaa cgggaacacg
      121 gtcgggtaca aaaagcagcg agtcgatttt caagacctgc tctatcagac gattcgcgcc
      181 ccgggcgcgc ccacgggcac cacgacgact tctcccaccg ggctccagct cggttctggc
      241 gtgcgcgtgg tcagcacgtc tcgcgtcttc tctcagggca cggtgctcac cacgaacaat
      301 ccgctcgacg tcgcgatcga aggcaacggc ttcttcgtcg ttcagcagaa cgacggcaag
      361 cccgcctaca cgcgcgccgg ggcgctccgt acggatccgc aaggccgcat cgtgacgccc
      421 gaagggcttc cgctcgatcc ccccgtcacg attccgcagg gcgcgaccaa catcagcatc
      481 gccgcgaacg gcaaggtgag cgtcacgctc ccgggtgaaa ccaacccagc cgacgtgggc
      541 caaattcaga tcgcgaactt catcaacccg gcgggcctgc agtccattgg gcacaacctc
      601 atgacgacgt cggccgcgag cggcgatgct caggtgggcg agcccggcac cgacggacgc
      661 ggcgctctgc tgcaaggcgc gaccgagcaa tccaacgtcg acatcgtcga agagatggtc
      721 ggcatgatca gcgcgcagcg cgcctacgag atcaactcga aggtgatcac caccgcggac
      781 gacatgctgc gcgctgccac ccaaatgagg taatcgcgga tgcgtccctt cctcgccgct
      841 ctttcgctct ctgccgctct gctcgggctc tcgcccgcgg cgctggcgga gcccacgcgc
      901 acgttggaga gcgctcgcat ccagctcgga caaatcgcgg agacgaccga cgccgagctc
      961 gccgccgtcg acctgggagc ctcgccgccg ccggggagtt cgcgcctgat ttcgcgcgag
     1021 gacgtaatgc gcgagcttcg agctcaggga ctcgaacagc gcaagctgag cttccccccc
     1081 gtgctccggg tcgtgagcgc ctcgcgtcgc tactcgccga gcgagctcag cgcgctcctc
     1141 ttgcccgaag tgaacgcggc cctacctccg ggcgtgaccc tgaaagagct gaaggcgcca
     1201 cgcgccctcg tgaccgcccc gcacgtcact gtcggtcgca tcaagctccc gaagctggcc
     1261 cgtcgcgcgg gcccggtcga cttgaccctc gtcgccgatc tgatgcacga cggcgccgtc
     1321 ctcacccgcg tcgcgtttgg tttggcagtc gacgtgagcg agcaggccgc ggccccgctc
     1381 gtcgacaagg gagcgcgcgt cgacctcgtc atcttgaccg gctccgcgcg catcagcgcg
     1441 agcgccgtcg cgctcgagcc cgctgacgtc ggcgacgtag tttcgttcaa agtgagcacc
     1501 acgcagcgcg tgctccgagc tcggctcgaa tctcgcacgc aagcgcaggt ggtgtcgcaa
     1561 tgaccaaatc gctcgctgcg ctcctgctcc tttccacgct gctcggcgcg tgcgggcccg
     1621 cgcacattgc gccgttcacg ccgcgcgagc gcaagttcga agccggtgaa tatgccgcca
     1681 ccaagaaggg ctctcagccc gccacgggct cgatatacag cgaagcccag cccggcttcc
     1741 tcgaagacac ccgcgcgctc cgtgtcggtg acatcgtgct cattcacatc aacgagcacg
     1801 ccgatgctca gggcggcgcg accacgaacc tgaagaagga aaccaaccgc gaagcggggg
     1861 tagacagctt cctcggtctc gtgcccgcca tccagaaggc gtaccccaac atcgatccga
     1921 ccaagctcct cgcgatggca tcgaaatcgg ggttcaacgg cgaaggcacc acccagcgcg
     1981 cgggcacgtt gaacggctcc atcggcgtcc acgtgaagca agagctgccg aacggcgacc
     2041 tctacgtcga gggcaccaag gtcgtgatga tcaatcacga ggagtaccac ctctacattt
     2101 ccggcgtgct ccgtccctcc gacatcgcgc ccgacaactc ggtcgactcg tcgctcatcg
     2161 ccgatgcgcg cgtcgagttc accggacgcg gcgacatcga cgaccaggtg aaccgcggtt
     2221 ggctcaccaa ggtcctcgac ttcatcaacc cgttctgaac catgcgttct cgcggcactt
     2281 tcacgtactc cttcgctcgc tggctctcgc tactcgcggt gctcgcgtgc atcggattct
     2341 cgccttcggc gcgcgccgat cggctacgcg acctctgcga cgtggtcgga gcccgcgaca
     2401 accaactgct cggttacggc atcgtcaccg gtttgaacgg caccggcgac gacctcacgg
     2461 ctccgttcgc gatgcaatcg ctgcgcgcgc tcctgcgccg cctgggcgtc caggtcgact
     2521 ccgggcagat gcgcttgaag aacatcgcgg cggtagtcgt cacggcgacg atccccccct
     2581 tcgcccgcaa cggctcgaag ctcgacatca ccatcgcgtc catcggcaac gcaaagtcgt
     2641 tgcgcggtgg cgtgctcctc caaacccctc tccacggagc cgaccgaaag acctacgccg
     2701 tcggacaagg cccaatcacc atcggtggct tctccgccgg cagcggcggc agctccgtgc
     2761 aagacggcgt gacgaccacc ggtcgaatct cgaacggggc gctc
//
LOCUS       DASTKS010000538         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_753221, whole genome shotgun sequence.
ACCESSION   DASTKS010000538 DASTKS010000000
VERSION     DASTKS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753221"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VFQ35_11145"
     CDS             <1..1058
                     /locus_tag="VFQ35_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931238.1"
                     /translation="ALAEQHRPSGIVLDVVLPDRSGLSVLDALKHNPSTRHIPVHMVS
                     GSDFAKTALEMGAAGYAIKPVAREQLVDALRKLEQKFTQKLRRVLVVEDDDAHRNGTC
                     RLLEADDVETLAVGTAAEALDKLRNVTFDCMVLDLSLPDRTGFDLLEEMQKEQYGFPP
                     VIVYTGKSLSREEEQKLQRFSQSIIIKGAHSPERLLDEVTLFLHQVESKLPPDRQRML
                     RDARHREAAFEGRRILVVEDDVRNIFALTSALEPRGAKVEIARNGREALTHLKEKPGV
                     DLVLMDIMMPEMDGLQATREIRKLPELAKLPIIALTAKAMADDRENCLAAGANDYIAK
                     PLDVDKLLSLARVWMPR"
     gene            1078..1899
                     /locus_tag="VFQ35_11150"
     CDS             1078..1899
                     /locus_tag="VFQ35_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9931239.1"
                     /translation="MIEPVDADAELELLLEAIYSKYHYDFRGYARASLRRRLRAALAH
                     FGCSTVDRLQELIWREPRAFSELLQYFTVQVSEMFRDPSYFRAVREKVVPYLKTYPSL
                     KLWVAGCSTGEEAYSFAILLAEEGLLDRTLIYATDINPEALRSAEDGVYDVSRFAQFS
                     QSYLQAGGRGSLSDYYTARYSSAVLDRTLRKSILFSDHSLATDSAFAEVEFASCRNVL
                     IYFERALQDRAIGVLTDSLRRKGFLGIGLKETLRFSAHAASYKEFSAPERIYQRL"
     gene            1899..>2489
                     /locus_tag="VFQ35_11155"
     CDS             1899..>2489
                     /locus_tag="VFQ35_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9931240.1"
                     /translation="MGKSNAKEPKAIVIGGSAGALDALTNILPALPADFALPIALVLH
                     LLPGKPSQLASVLSLHTRLSVRETDDKQPFTPGTIYVAPPNYHLLLEKTGCLSLSVED
                     PVLFSRPSIDVLFESAAEAYGPSAVGVLLSGANEDGAAGLRGIAAAGGITIVQHPDTA
                     TARAMPEAALQLFKPHHVLPLAGIGALLARLPHATAE"
BASE COUNT          442 a          851 c          756 g          440 t
ORIGIN      
        1 tcgccctcgc cgagcaacac cgcccgagcg gcatcgtgct cgacgtcgtg ttaccggacc
       61 gctccgggct ctcggtgctg gacgccttga agcacaaccc ttccacgcgg cacatccccg
      121 tccacatggt ctccggctcc gatttcgcga agaccgcgct cgaaatgggt gccgccggct
      181 acgcgataaa gccggtcgcg cgggaacagc tcgtggacgc gctgcgcaag ctcgagcaga
      241 agttcacgca gaagctccgc cgtgttctcg tcgtcgagga cgacgacgcg catcgtaacg
      301 ggacttgtcg gctgctcgaa gcggacgacg tggagacgct ggcggtgggc acggcggcgg
      361 aagcgctcga caagctccgc aacgtgacgt tcgactgcat ggtgctcgat ttgtcgctgc
      421 cggatcgaac cggcttcgac ctgctcgagg agatgcaaaa ggagcaatac ggcttcccgc
      481 ccgtcatcgt gtacaccggc aagagcctct cacgcgaaga ggagcagaag ctgcagcgct
      541 tctcccagtc gatcatcatc aaaggcgccc attcgcccga gcgcttgctc gacgaggtca
      601 cgctcttttt gcatcaagtc gagtccaaat tgccgccaga ccggcagcgc atgctgcgtg
      661 acgcgcggca ccgcgaagcc gcgttcgagg gtcgccgcat cctcgtcgtc gaggacgacg
      721 tgcgcaacat cttcgcgctc acgagcgccc tcgagccacg cggagccaag gtggagatcg
      781 cccgcaacgg gcgcgaggcg ctgacgcacc tgaaggagaa gcccggcgtc gatctcgtct
      841 tgatggacat catgatgccc gaaatggacg gcctccaagc gacgcgcgag atccgcaagc
      901 ttccggagct cgcgaagctg ccaatcatcg cgctcaccgc aaaggccatg gccgatgacc
      961 gcgaaaactg cctcgccgcg ggagccaacg actacatcgc gaagccgctc gacgtcgaca
     1021 agctgctctc gctggcgcga gtctggatgc cgcgatgaac ggcgccgggg tagccgcttg
     1081 atcgaacccg tcgacgccga cgcggagctc gagcttctcc tcgaggcaat ttactcgaag
     1141 tatcactacg actttcgcgg ctatgcccgc gcttcgctgc gacgccggct gcgcgctgcg
     1201 ctcgcacact tcgggtgctc gacggtcgac agactgcaag agttgatctg gcgcgagcct
     1261 cgcgctttca gcgaactctt gcagtacttc acggtgcagg tcagcgagat gttccgcgac
     1321 ccaagctact tccgcgctgt ccgcgagaag gtggtgccgt acctgaagac ttacccgtcc
     1381 ttgaagctgt gggtcgccgg ttgttcgacc ggtgaggagg cgtattcgtt cgccatcctg
     1441 ctcgcggagg aggggttgct cgaccgcacc ctgatctacg cgaccgacat caaccccgaa
     1501 gccctacgct cggccgagga cggagtctac gatgtctctc gtttcgcgca gttcagccaa
     1561 agctacctcc aggcaggcgg tcgcggctcg ctctcggatt actacaccgc gcgctattcc
     1621 tcggccgtgc tggaccgcac gctgcgcaaa tcgatcttgt tctccgacca cagccttgcg
     1681 accgatagcg ccttcgcgga ggtcgagttc gcgtcgtgcc ggaacgtact catctacttc
     1741 gagcgcgccc tgcaagatcg cgccatcggc gtgctcaccg actccctgcg acgcaagggg
     1801 ttcctcggca tcggtttgaa ggagaccctg cgcttctcgg cccacgccgc ttcgtacaag
     1861 gaattttccg cgccagagcg catctatcag aggctttgat gggaaagagc aacgcaaagg
     1921 aaccgaaggc gatcgtcatc ggtggctcgg ccggtgcgct cgacgcgctg acgaacattt
     1981 tgccggcgct cccggctgac ttcgcgctgc cgatcgcact cgtcctgcac ctcttacctg
     2041 ggaagccgag ccagctcgcg agcgttctct cgctccacac gcgcctttcg gtccgcgaaa
     2101 cggatgacaa gcagccgttc acgcctggca ccatctacgt ggcgcccccg aattaccacc
     2161 tattgctcga gaaaacgggc tgcctgtcgc tctccgtcga ggacccggtc cttttctctc
     2221 gcccgtccat cgacgtcctc ttcgagtcgg ccgccgaagc gtacgggcct tccgcggtgg
     2281 gagtcttgct gagcggcgcc aacgaggacg gcgctgcggg cctgcgcggc atcgccgcgg
     2341 cgggaggcat tacgatcgtg caacacccgg acactgcaac ggcgcgcgcc atgccggaag
     2401 ccgcgctgca gctcttcaaa cctcaccacg tgctacctct ggcaggcatc ggcgcgctac
     2461 tcgcccgctt gccgcatgcg accgcggag
//
LOCUS       DASTKS010000539         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_976904, whole genome shotgun sequence.
ACCESSION   DASTKS010000539 DASTKS010000000
VERSION     DASTKS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976904"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFQ35_11160"
     CDS             complement(<1..202)
                     /locus_tag="VFQ35_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931241.1"
                     /translation="MGSFALPRTLSLVKSFAASSALVACAAAPAPTPRCEATAPALSS
                     PVPTAPAPNGTPAPPVAPAAPAS"
     gene            complement(285..668)
                     /locus_tag="VFQ35_11165"
     CDS             complement(285..668)
                     /locus_tag="VFQ35_11165"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HET9931242.1"
                     /translation="MFKRLFGASCALACVLLVGESARADGMQCGDSLVTTGDSLYQVR
                     STCGDPDAAYHRVEYRTVRHRVAAPCVHENGRVRCETVIEQTVEVHIDEWTYDFGKDR
                     FIEYLTFEQGQLVRVNEGSYGHKHR"
     gene            complement(872..1552)
                     /locus_tag="VFQ35_11170"
     CDS             complement(872..1552)
                     /locus_tag="VFQ35_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HET9931243.1"
                     /translation="MAFSGFADEKAKFFHALAKNQSREWFQAHKVEFEEGYQAPLKEL
                     LSEVRDGIDPAYKHSELDEPRTFRINRDVRFSKDKSPYKTHAGGIIPTKRQGKITEVP
                     MALYFHVGLPASFGAAGHYMMDPRSLSGFRAAVHDEKRGRELVKLLTALQKKGFRVDS
                     HDTYKRVPKGFDPEHPRAEHLKRKGLTVAFPDLPKGILKSPKLVPWVVTQAKAAAPLV
                     EWLIFATI"
     gene            complement(1573..2595)
                     /gene="cydB"
                     /locus_tag="VFQ35_11175"
     CDS             complement(1573..2595)
                     /gene="cydB"
                     /locus_tag="VFQ35_11175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET9931244.1"
                     /translation="MPELWYALLALMLTAYAVLDGFDFGAGILHLFVARNESERREVF
                     AAIGPFWDGNEVWLIGAGGVMFLAFPVLLATAFPAFYLSLFVVLWCLLLRGIAIELRS
                     HVNNAEWRTFWDAVFALSSALLALLFGVALGNVVRGLPLDAEEVTTLPLFTDFGVSGR
                     VGLIDWYTLLVGVFSVGLLTSHGALFLQYRSRSDLTTRAHRVERALRWPLLGLFIGVS
                     VATHLVRPDFFVALATRPLAWFALGIGLVGGVLASREAGQGGRRFVGSCLRISGLLGA
                     LAVAVFPTLLRSTLDPAHSLTLGQAMSGDYSRRVALFWWPLAFVLAVAYLALAFWTHR
                     APVTRE"
     gene            complement(2588..3970)
                     /locus_tag="VFQ35_11180"
     CDS             complement(2588..3970)
                     /locus_tag="VFQ35_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET9931245.1"
                     /translation="MVVSLLVDDPGFWHRVQFGFTIAFHYLFPQLTMGLALLVFLLKW
                     YALKKHDMLAADAARFWTRIFGINFAMGVATGVPMEFQFGTNWSRFSRYAGGVVGQTL
                     ALEGMFAFFLESAFVAALVWGERRLGPRRHLGAAFAVLLGSWLSAYFIVTTNAFMQHP
                     VGYALENGRLVLNDFWAFLFNPWALLQFAHTLAASVVTAAFVMTAVGAFYTLGGKHLP
                     AARLFLRWGTAAGLVAALFVAFPTGDRQAKAVAEHQPVTLAAMEGRFESGPWAELTFL
                     GQPNLGTGRIDNAIQAKGVLSILAYGTPRRSVKGLRDFPVEEWPSNVELLYYSFHIMA
                     GLGTLFIALMALANFARFRGRLESWRGLLWALMLAFPFPYIANTAGWLTAELGRQPYL
                     VYGLFRTLEGYSRGVSSGNALFTLLGFAGLYFVLGLLFLFLVAREIAHGPGGAAPTEY
                     GVPAEAEGHA"
BASE COUNT          852 a         1357 c         1314 g          645 t
ORIGIN      
        1 cgctcgcggg cgcggctggc gcgacggggg gcgcgggcgt gccattcgga gccggtgctg
       61 ttggtacggg gctcgagagc gctggagccg tcgcttcaca gcgcggggtg ggcgcgggcg
      121 cggcagcaca tgcaacgaga gccgaagacg cggcgaacga tttgaccagc gaaagggtgc
      181 gaggaagagc gaacgaaccc atcctgagct cgatagcgca agtcaaaggc ccgtggtcga
      241 ctttctctgc gcgagacgaa actcgccacc cgcggggacg aacgtcagcg gtgcttgtgc
      301 ccgtagctcc cctcgttgac gcgcacgagc tgaccttgct cgaaggtcag gtactcgatg
      361 aagcggtcct tgccgaagtc atatgtccac tcgtcgatgt ggacctcgac cgtctgctcg
      421 atgacggtct cacaacgaac tcgcccattc tcgtgaacac agggcgccgc gacccgatgc
      481 cttacggtgc gatactccac ccggtggtag gcggcatcgg gatccccgca tgtagagcgc
      541 acttggtaga gcgagtcgcc ggtcgtaacc aggctgtctc cgcactgcat gccgtcggcg
      601 cgcgcgctct cgccgacgag caggacacac gccaaagcgc aactggcacc gaacagacgc
      661 ttgaacatat cggcttctcc cgagccaccc agcccggaac tgggcatgaa tccgggcggc
      721 cagcgctcag acgtccgccc ggcttcttct attcaagccc gcatgcttcg ttcgaggccc
      781 acgtcccccc ggcttcttct attcaagccg gcccgcgtct tcccaccgag gccgttcgac
      841 gcgcgagatc tcccgttcgg cgcggcttcc ctcagatcgt cgcgaaaatc agccactcga
      901 cgagcggcgc ggctgctttc gcctgggtca cgacccatgg cactagtttc ggcgacttca
      961 atatcccttt ggggaggtcg gggaacgcta cggtcaagcc tttacgcttc aggtgctctg
     1021 cccgggggtg ctccgggtcg aaccccttcg gcacccgctt ataggtgtcg tgggaatcga
     1081 ctcgaaagcc ctttttttga agggcggtca gcaatttgac gagctcacgg ccgcgctttt
     1141 cgtcgtggac cgcggcgcga aacccactga gcgagcgggg atccatcatg tagtgaccgg
     1201 cagctccgaa cgaggcgggt agcccgacgt ggaagtagag agccatgggc acctccgtga
     1261 tcttgccctg gcgcttggtc gggatgattc cccctgcgtg cgtcttgtac ggcgacttgt
     1321 ctttcgaaaa gcgcacgtcg cggttgatcc ggaatgtcct cggctcgtcg agctcggagt
     1381 gcttgtacgc tggatcgatt ccgtcgcgaa cctcggagag aagctccttg agcggggcct
     1441 gatacccctc ttcgaattcg accttgtgcg cttgaaacca ttcacgcgat tggttcttgg
     1501 cgagcgcgtg gaagaacttg gccttctcgt cagcaaaccc tgaaaacgcc atgccgactg
     1561 actacactcg attcactctc gcgtcaccgg ggcgcgatgc gtccagaacg cgagcgctag
     1621 ataggcgacg gcgagcacga aggcgagcgg ccaccagaag agcgcgacgc ggcgtgagta
     1681 gtccccgctc atggcctggc cgagcgtgag cgaatgcgcc ggatccaacg tggaccgcag
     1741 cagcgtcggg aacacggcca cggcgagcgc gccgagtaga cccgaaatgc ggaggcacga
     1801 cccgacgaag cgacgccctc cctgtcccgc ttcccgcgac gcgaggacgc cgccaacgag
     1861 cccgattccg agggcgaacc aagcgagcgg gcgggtcgcg agcgccacga agaagtccgg
     1921 gcgcacgagg tgagtggcga cgctgactcc gatgaagagg cccagcaagg gccaacgcag
     1981 cgcgcgttcg acgcggtgcg cgcgcgtcgt aaggtcgctt cgactccgat actgaaggaa
     2041 cagggctccg tgcgacgtga ggagcccgac gctgaacacc ccgaccaaca gcgtatacca
     2101 gtcgatcagg ccgactcgcc cgctcactcc gaaatcggtg aacaagggaa gagtggtcac
     2161 ctcttcggcg tcgagcggca acccccgcac gacgttgccg agcgctactc cgaacagcaa
     2221 cgcgagcagc gcgctcgaca gcgcgaacac cgcatcccag aaggttctcc actcggcgtt
     2281 gttcacgtgg ctccgaagct cgatggcgat ccctctgagt agaaggcacc acaacacgac
     2341 gaacaggctc aagtagaagg ccgggaatgc cgtggcgagc aggaccggga aggcgaggaa
     2401 catcacgccc cctgcaccga tcaaccagac ttcgtttccg tcccagaaag gccctatcgc
     2461 cgcaaagacc tcgcgacgct cggactcgtt cctcgcgacg aacaagtgca agatcccagc
     2521 tccgaaatcg aagccgtcga gcacggcgta cgcggtcagc atcaacgcga gcagcgcgta
     2581 ccagagctca ggcatggcct tctgcctccg cggggacacc gtactcagtc ggggcggcgc
     2641 cgcccggacc gtgagctatc tcccgcgcga cgaggaacaa gaacaagaga ccgagcacga
     2701 agtagagccc cgcgaagccc agtaacgtga agagcgcgtt ccccgagctc acgccgcggc
     2761 tataaccctc tagggttcgg aacaacccgt aaacgaggta cggctgtcgc ccgagctctg
     2821 ccgtcaacca ccccgccgtg ttggcgatgt aggggaatgg gaacgcgagc atcagcgccc
     2881 acaaaagtcc gcgccacgac tcgagccgcc cgcgaaaccg agcgaagttc gcgagcgcca
     2941 tcaaggcgat gaacagggtg ccgagcccag ccatgatgtg gaacgagtag tagaggagct
     3001 cgacgttact gggccattct tcgaccggga agtctcgcaa gcccttcacg ctccgacggg
     3061 gagttccgta agcgaggatg ctgaggacgc ccttggcctg gatggcgttg tcgatgcgcc
     3121 cagtgccgag gttcggctgc ccgaggaagg ttagctctgc ccacggcccg ctctcgaagc
     3181 gcccctccat cgccgcgagc gtgaccggtt ggtgttcggc gaccgccttg gcctgccggt
     3241 caccggttgg aaatgcaacg aacagcgcgg ccacgagccc cgcggcggtc ccccaccgca
     3301 ggaacaggcg cgccgccggc aggtgcttgc ccccgagcgt gtagaacgcg ccaaccgccg
     3361 tcatcacgaa cgcggccgtg acgacgctgg cggccaatgt gtgcgcgaac tggagcaacg
     3421 cccacggatt gaacagaaac gcccaaaagt cgttcagcac tagacgcccg ttctcgagcg
     3481 catacccgac cgggtgctgc atgaaggcgt tggtagtgac gatgaagtaa gcggagagcc
     3541 aactaccgag cagcaccgcg aacgctgcgc cgaggtgtcg ccgcgggcct agccggcgct
     3601 caccccacac gagcgcggcg acgaaggcgc tctcgagaaa gaacgcaaac attccctcga
     3661 gcgcgagcgt ttgacccacg acgccgcccg cgtagcgcga aaagcgagac cagttcgtgc
     3721 cgaactgaaa ttccatcggc acccccgtcg cgacgcccat cgcgaagttg atgccgaaga
     3781 tgcgagtcca gaagcgcgcg gcgtcggccg cgagcatgtc gtgcttcttc agcgcgtacc
     3841 acttcaacaa aaagacgagg agcgcgaggc ccatcgtgag ctgcggaaac agatagtgaa
     3901 acgcgatcgt gaaaccgaac tgcacgcggt gccaaaagcc agggtcgtcc acgaggagcg
     3961 agactaccac ccgtattcga acgtgcgaca tcattgcctc gagtacgcgc tcggaagaca
     4021 cgctcgaacg caaatccagg cgtctcggtg agcgcgggtt tctcttggcg gagtttcggc
     4081 gactcgttta gattgaagaa cgctggggca catttcccct gcgcacggca tgagttttcc
     4141 ggcaaccgac aaagccccgg aacaacag
//
LOCUS       DASTKS010000540         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_976921, whole genome shotgun sequence.
ACCESSION   DASTKS010000540 DASTKS010000000
VERSION     DASTKS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976921"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1232)
                     /locus_tag="VFQ35_11185"
     CDS             complement(81..1232)
                     /locus_tag="VFQ35_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9931246.1"
                     /translation="MARVPPLGIRRIEAIHYYVRDLERSRRFYTERLDFAEIGQSSAQ
                     LVEGGHQRSLVFEGGSCRIVCSAPVGEGGRAHRYLQKHPDGVGSIIFEVADIERTFAF
                     LDARGGTPIHEIRTTTGPGGEFKTFLITTPFGDTTFRFIERKGSFPLYPGFETYVAPR
                     GGANSLGFVCFDHVTSNFETMTPALLWMEHVLGFERFWQVEFHTDDVKKRVDEAGSGL
                     RSVVMWDPESGVKFANNEPYRPNFKASQINVFHEENRGDGVQHVALCVTDIVSAVRAL
                     RARGVEFMPAPSAYYDALPSRLAALGIGELREPIDELRKLELLVDGDGRGRYLLQIFL
                     KDSAGLYKSPEAGPFFYELIQRHGDEGFGAGNFRALFESIETEQSRGSR"
     gene            1411..3537
                     /locus_tag="VFQ35_11190"
     CDS             1411..3537
                     /locus_tag="VFQ35_11190"
                     /inference="COORDINATES: protein motif:HMM:NF039970.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HET9931247.1"
                     /translation="MLRVPVAPGIFAVALGALACGASGSEASGSGGRASGGSTGNAGG
                     SAGGVTNGSGGSSRPASGGNAGVATSGGAADGGTVSSGGRSNGGSAGSSIGRGGSVNA
                     NGGSGSSSGGTSAGGSTSGGAASGGVPATCDPGSTTTQWATQCPTSGPTCTAGTWMAG
                     GPDPDHQSYKLIKESAHFAIYSDESVSDTSAQTALETLENLIWTVYFGNPIYFKEPLC
                     NQATKYKVSVHVNSANGLTGGAWSSNRMGMWIGPGALADHWGLAHEFMHGVQSVSGGM
                     SCGGGSNYCGWIYESHANFMPHQIAEYRNDVHCSEMSFNMPHLYLGSTRDRYCNWQFM
                     EFLKDKYCYSAVNAIWTQSAANDPFTNIMKGLDWNISQLNDFIGEWAMHNVTWDYKNP
                     EPKLAGDNSDPAANFRAKYGLITDTSQAIRRLRTTMLEPLDASPAQNHRFASPFYWAP
                     QRFGYNVVRLFPDGGVSNVRVTFRGVTQAGPNPDWRWGLVATNSALTTARYSALQKGA
                     EAELDFCVGAGESLWLVVAATPSVQQHIVWDQPYATVPRYPYMVAFEGAWPDGFQGGA
                     QAACPSGLSRAKNGGGCAPASIPDTVFVGPYATVLSGATVSGNARVEDHATVVSGTVS
                     GGTVGAMSLISGFKMSSGTVKTTFYPLGFFENNQGLSGGTLVGDVEYRGAGLSRSSGT
                     CAGFVDSGTCVAPGTDATPAPPYQWR"
     gene            complement(3547..>4429)
                     /locus_tag="VFQ35_11195"
     CDS             complement(3547..>4429)
                     /locus_tag="VFQ35_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931248.1"
                     /translation="DDAFNLDLLQEFLRGADYDVIAVPSGAAALETLERHPDIDVVVL
                     DRMMPAPNGMEVLERMKRHPTWASIPVVMQTAAGHREQMLEGMRAGAYYYLVKPYEEA
                     MLLAMVKAALSDEQQRVVRDELRILRAGFRLAARVDFRFRTVAEARDVSDFIARCFPT
                     ITDLRLGLAELTTNAIEHGNLGITYAEKSELLIRDAWEAEVERRQQLPENLCKYAELN
                     LEISAHRLSIRLRDKGNGFDWSKYQEFSVERAADPHGRGIAMARALAFDSLEYIGNGS
                     ELQCSVSLSPLSDGSTK"
BASE COUNT          789 a         1405 c         1463 g          772 t
ORIGIN      
        1 gtaaatgggt gagccgtcca gaccgcgaac gacgaggtgc ggcttctccg gaacgtcccc
       61 gtgaaccgcg cggtcgagca tcagcgacta cctctcgact gctccgtctc gatgctctcg
      121 aacagcgcgc ggaaattacc cgcgccgaag ccctcgtcgc cgtgacgctg gatgagctcg
      181 tagaagaacg gtcccgcttc ggggctcttg tagagccctg ccgagtcttt cagaaagatc
      241 tgcagcaggt agcggcctct gccatcgccg tctacgagaa gctcgagctt tctgagctcg
      301 tcgatcggct cacgcagctc accgatgccg agcgcggcga gacgcgacgg caaagcatcg
      361 tagtaggcag acggcgccgg catgaactcg acgccgcgtg cccggagcgc gcgcacggcg
      421 ctcacgatgt cggtcacaca gagcgctacg tgctgcactc cgtcgccgcg gttctcctcg
      481 tggaagacgt tgatttgcga agccttgaag tttggtcgat aaggttcgtt gttggcgaac
      541 ttcacgccgc tctcgggatc ccacatgacc accgagcgca gcccggagcc ggcctcgtcg
      601 acgcgtttct tgacgtcatc ggtgtggaac tcgacttgcc aaaagcgctc gaacccgagg
      661 acgtgctcca tccacaagag cgcgggtgtc atcgtctcga agttgctcgt gacgtggtcg
      721 aagcagacga agccgagcga gttcgcgcct ccgcgcgggg cgacgtacgt ctcgaagccg
      781 ggatacaacg gaaagctccc cttgcgctcg atgaagcgaa acgtagtgtc gccgaatggg
      841 gtcgtgatga ggaaggtctt gaattcgccg ccgggccctg tggtcgtgcg aatctcgtgg
      901 atgggggtgc ctccccgcgc gtccaagaac gcgaaggttc gctcgatgtc cgcgacctcg
      961 aagatgatgc tgcccacgcc gtccgggtgc ttctgcaagt agcggtgcgc gcggccgcct
     1021 tcacccactg gcgccgagca cacgatgcga caacttcccc cttcgaagac gagagaccgt
     1081 tggtgtcctc cttcgacgag ctgcgccgag ctttgcccga tttcggcgaa gtcgagccgc
     1141 tccgtataga acctgcggct cctctccaaa tcgcggacgt agtagtggat ggcctcgatg
     1201 cgacggatcc cgagcggggg aacgcgagcc atgcagccag tctacccctc gtacagggtt
     1261 cgaccagcgc cgtggctgtg cgtcgaggta agtgccccgc cggaaggctc ggcgggcgcg
     1321 cagcgcgacg gagtccacgg aaagttgacc agccgcggcc agcctccgtc actacaccct
     1381 gcgactcgtc gcctcggagg aactctgcgt atgcttcgtg tacccgtcgc tcctggaatc
     1441 tttgcggtcg cgctcggcgc attggcttgc ggggcgagcg gctccgaagc atccggctcc
     1501 ggcggccgag cgagcggcgg cagtacgggg aacgcaggtg ggagcgccgg cggcgtcacg
     1561 aacggcagtg gaggttcgtc tcggccggcg agcggcggta acgcgggagt cgcgacgagc
     1621 ggcggtgcgg ctgacggcgg cacggtcagc agcggtggtc gaagcaatgg cggtagcgcc
     1681 ggctcgagta ttggtcgcgg tggttccgtg aacgccaacg gaggcagcgg ctcgagcagc
     1741 ggcggaacga gcgctggtgg aagcacgagc ggcggcgcgg cgagcggcgg cgttcccgcg
     1801 acgtgcgatc ccggctcgac gactacgcag tgggcgacgc agtgccccac ctcgggtccg
     1861 acgtgcacgg ccggcacctg gatggcgggt ggccccgatc ccgatcacca gagttacaag
     1921 ctcatcaagg agtccgcgca tttcgcgatt tactcggacg agagcgtctc cgacacgagc
     1981 gcgcaaacgg cgctcgagac gctcgaaaac ctgatttgga ccgtctattt cgggaacccg
     2041 atctacttca aagagccgct ctgcaaccaa gcgacgaagt acaaagtgag cgtgcacgtg
     2101 aactccgcga acgggctcac cgggggagct tggtcctcga accgcatggg aatgtggatt
     2161 gggcctggag cgctcgcaga tcactggggt ttggcacacg agttcatgca cggtgttcag
     2221 agcgtgagcg gcgggatgag ctgcggcgga ggctcgaact attgcggctg gatctacgag
     2281 agccacgcga acttcatgcc ccaccagatc gcggagtacc ggaacgacgt gcactgctcg
     2341 gagatgtcgt tcaacatgcc gcacctttac ctgggctcca cgcgcgaccg ctactgcaac
     2401 tggcagttca tggagttcct gaaggacaag tattgctact ccgccgtgaa cgcgatttgg
     2461 acacagtccg ccgcgaacga tccgttcacg aacatcatga aagggctcga ctggaacatc
     2521 tcgcagctga acgatttcat cggcgagtgg gccatgcaca acgtgacttg ggactacaag
     2581 aacccagagc cgaagctcgc cggcgacaac agcgacccgg ccgcgaactt tcgagccaag
     2641 tacgggctca tcaccgatac gtcacaagcg atcaggcggc tgcgcacgac gatgctcgag
     2701 ccgctcgacg cgagcccggc gcagaatcac cgcttcgcgt cgccctttta ctgggcaccg
     2761 caacgcttcg gctacaacgt cgttcggctc ttcccggatg gtggggtgag caacgtacgc
     2821 gttacgtttc gcggcgtgac tcaggccggg ccgaaccccg actggcgctg gggcctcgtc
     2881 gcgacgaact cggcgctgac cacggcgcgc tacagcgcgc tgcagaaggg cgccgaagcc
     2941 gagctcgact tttgcgtcgg cgcgggcgag tcgttgtggc tcgtggtggc ggcgacgcct
     3001 tcggtgcagc agcacatcgt ttgggatcag ccgtacgcga ccgtgccgcg ctatccgtac
     3061 atggtcgcgt tcgaaggcgc ctggcccgac ggtttccaag gtggtgctca agcggcgtgc
     3121 ccgagcgggc tctcgcgcgc gaagaacggg ggaggctgcg ctccggccag cattcccgat
     3181 accgtcttcg tcggtccata tgcgacggtg ctgagcggcg ccaccgtttc cggcaatgcg
     3241 cgcgtcgagg accatgcaac cgtagtgagc ggtaccgtct cgggcggcac cgtcggtgcg
     3301 atgtcgctca tcagcggctt caagatgagc agcggcaccg tcaagacgac gttctacccc
     3361 ctcgggttct tcgagaacaa tcaagggctc tccggcggta cattggtcgg agacgtcgag
     3421 taccgaggcg ctgggctcag ccgtagttct ggcacgtgcg ccggcttcgt agacagcggc
     3481 acgtgcgtgg cgcccggcac cgacgcgact cccgcgccgc cgtaccaatg gcgctgagcg
     3541 acggcatcat ttcgtcgaac cgtcgctcaa agggctgagc gagacgctgc actggagctc
     3601 gctgccgttt ccgatgtact cgagagaatc gaaggcgagg gcgcgcgcca tcgcaatgcc
     3661 tcgcccgtgc ggatccgcgg cgcgttctac cgagaactct tggtacttcg accagtcgaa
     3721 gccgttccct ttgtcccgca aacggatcga gagccgatgg gcggagattt cgaggttcag
     3781 ctcggcgtac ttgcacaggt tttcgggcag ttgctggcga cgctcgacct ccgcttccca
     3841 ggcgtcgcgg atgaggagct ccgacttttc ggcgtacgtg atgcccagat ttccgtgctc
     3901 aatggcattg gtcgttagct ccgcgaggcc gagtcgcaaa tccgtgatcg tcggaaagca
     3961 gcgcgcgatg aagtcgctca catcgcgcgc ctccgcgacg gtgcgaaacc gaaaatcgac
     4021 gcgcgccgcg aggcggaagc ccgcccgcag gatccgcagc tcgtcgcgca ccacgcgctg
     4081 ctgttcgtcg ctcagcgcgg ccttgaccat ggcgagcagc atcgcctctt cgtatggctt
     4141 gaccaggtag taataggcgc cggcgcgcat gccctcgagc atttgctctc ggtggcccgc
     4201 ggcggtctgc atgactaccg ggatgctcgc ccaggtggga tgccgcttca ttcgctccag
     4261 cacctccatg ccgttgggcg ccggcatcat gcggtcgagt accaccacgt cgatgtctgg
     4321 atgccgctcc agagtctcca gcgccgccgc gccgctcggc acggcgatga cgtcgtagtc
     4381 ggcgccacgc aggaactctt gtaaaaggtc gagattgaag gcgtcgtca
//
LOCUS       DASTKS010000541         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_85126,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000541 DASTKS010000000
VERSION     DASTKS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85126"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..789
                     /locus_tag="VFQ35_11200"
     CDS             220..789
                     /locus_tag="VFQ35_11200"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9931249.1"
                     /translation="MSTARRLHHSYEDYLHALEMSELKLEYCEGEIYAMAGGTPTHAD
                     LAASMTRLIGNALLGRCRVSSSDLKVRLEATGLSTFPDVTVVCGERMLSPIDRNAIVN
                     PTVVVEVTSQSTEDYDRGEKLNHYQQCPSVRAVIFVSHRRPQLTLLRRTPGGFEQSEH
                     HTGESLELDEPRLSLSVDEVYAGIALDDG"
     gene            814..1743
                     /locus_tag="VFQ35_11205"
     CDS             814..1743
                     /locus_tag="VFQ35_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9931250.1"
                     /translation="MASPRTKKQKQPEGPAPVVLFDTYSLLFRAHHALPPMNTATGEP
                     TSALYGFSAVVLKVLKEQRPRAFAFALDAPRRTFRHEAYADYKGSREAAPSVLVEQLK
                     RLPWLLEAFGVPVMCVPGFEADDVLATLATKLCARDEAALVVSGDRDMLQLCNERCRV
                     YFVGARGQEPTLFDVEKVRERFGVEPAQMPTRAALVGDASDNLPGVPGIGPQTAAKLV
                     QEFGSVEGLLARVDEVNPERIREALRAHAEQIRLNEDLSRLRRDVDLPEGELAKSLSA
                     DSFVSLGKAFEALEFKSLLTRLAALEGVLRATP"
     gene            complement(1744..>2839)
                     /locus_tag="VFQ35_11210"
     CDS             complement(1744..>2839)
                     /locus_tag="VFQ35_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9931251.1"
                     /translation="NEQVAGNIVELASSVRETVASIEEMAYSIKEVAKNVDALSLTAE
                     ETSSSMNEMDVSIDQVQSNANETARLSEEVAQDAERGAEAILKTIGEIYRIKESSQEA
                     VAVISNLGSRIDAIGQILNVIDDVAEQTNLLALNAAIIAAQAGEHGKGFAVVADEIKD
                     LAERAGASTKEIADLIKTIQTESKNAIQAVERGALNVDRGVEVSNEAERALKKILESS
                     QKSTNMVRAIARATVEQAKGSKQVTDAIGRIAATVQQIAAATAQQARGSELIMKSGEK
                     MRTIAQQVERSTHEQSNGSRQLKQAIENINGMVSQLNTAHRSLHRGSERALAAANQLE
                     EAARRQDSACRELSTHLDRMKKTVDERSAS"
BASE COUNT          431 a          871 c          972 g          565 t
ORIGIN      
        1 gcgtgccctc accgtgtggc ggcgttgcgc gaccttgggc gcgaatgagc gccgcgcgcg
       61 cgagttcctt ctggggttcg atgcttcgcg cgggccagca cttcgacctc accgggagca
      121 acgcgtcgag cattggtctt agagcgccga acggtgacga agcagaacta gcgccgggcc
      181 gagcggcgtc ggtggcgatt gaatgctatg acggactgag tgagcaccgc acgtcgactc
      241 caccacagct atgaggacta cctccacgct ttggagatga gcgagctcaa actcgaatac
      301 tgcgagggcg agatctatgc aatggcaggg ggcacgccga cgcatgctga ccttgccgcg
      361 tcgatgaccc gcctgatcgg aaacgcactc ttgggaaggt gccgcgtctc ttcttcggac
      421 ttgaaggtgc gcctcgaggc tacgggtctc tccacttttc cggacgtaac cgtcgtttgc
      481 ggtgagcgaa tgctctcgcc catcgaccgc aacgcaatcg tgaatccgac cgtggtggtg
      541 gaggtgacga gtcagtccac cgaggactac gaccgcgggg agaagctgaa tcactaccag
      601 caatgtccga gcgtacgcgc ggtgatcttc gtgtcacatc ggcggccaca gctcacgttg
      661 ctgcgacgaa cgcctggagg gttcgagcag agcgagcatc acacgggaga gtcactggaa
      721 ctcgacgagc cgcgcctttc gctgagcgtc gacgaagtgt acgcaggcat cgcgctcgac
      781 gacggctgaa gcagcctcgt gctacgtagc gccatggcgt cgccgcgcac gaagaaacag
      841 aagcagcccg agggacccgc gccggtcgtc ttgttcgata cgtattcgct cttgttccgc
      901 gcgcatcacg ccttgccgcc gatgaacacg gcgacgggcg agccgacctc ggcgctgtac
      961 ggattttcgg cggtggttct caaagtgctg aaggagcagc ggccccgggc gttcgccttc
     1021 gcgctggacg cgccgcgacg gacttttcgc cacgaggcgt acgcggacta caagggctcg
     1081 cgcgaggcgg cgccctcggt gctcgtcgag cagctcaagc gtttgccgtg gctgctcgag
     1141 gcgtttggcg tgccggtgat gtgcgtgccg ggtttcgaag cggacgacgt gctcgcgacg
     1201 ctggcgacca agctttgcgc gcgcgacgaa gcggcgctcg tggtgagtgg cgatcgcgat
     1261 atgttgcagc tctgcaacga gcgctgccgt gtgtacttcg tgggggcgcg cggccaggag
     1321 ccgaccctgt tcgacgtcga gaaggtgcgg gagcgctttg gagtggagcc ggcgcaaatg
     1381 ccgacgcgcg ccgcgctggt cggcgatgcc tccgacaatt tgccgggagt gcctggaatt
     1441 gggccgcaga ccgcggcgaa gctggttcag gagttcggga gcgtcgaggg gctactcgcg
     1501 cgcgtcgacg aggtgaaccc cgagcgcatt cgggaggcgc tccgcgcgca cgccgagcaa
     1561 attcgtctga acgaagacct gagccgtttg aggcgcgatg tcgacttgcc cgaaggagag
     1621 ctcgcgaagt cgctctccgc ggactctttc gtaagcctcg gcaaagcctt cgaagcgctc
     1681 gagttcaaga gcctgctcac gcgcttggcc gcgctcgaag gcgttctgcg cgcgacgcct
     1741 tgactagctc gcggagcgct cgtcgaccgt cttcttcatg cggtcgaggt gcgtcgaaag
     1801 ctcgcgacac gccgaatctt ggcgacgcgc ggcctcttcg agttggttag cggcggcgag
     1861 ggcgcgctcg gagcctcggt gcagcgagcg gtgcgcggtg ttgagctggc tcaccatccc
     1921 gttgatgttc tcgatcgctt gcttgagttg gcggctaccg ttgctctgct cgtgggtcga
     1981 gcgctccacc tgctgagcga tggtgcgcat cttctcgccg ctcttcatga tgagctccga
     2041 gccgcgcgcc tgttgcgccg tagcggcggc gatctgctgc accgtcgccg cgatgcgacc
     2101 gatggcgtcg gtgacctgct tgctgccctt cgcttgctcg acggtggcgc gagcgatggc
     2161 gcgcaccatg ttggtgctct tctgcgagct ctcgaggatc ttcttcaggg cgcgctcggc
     2221 ctcgttcgag acttcgacgc cgcggtcgac gttcaaggcg ccgcgctcca ccgcctggat
     2281 ggcgttcttg ctctcggtct ggatggtctt gatgagatcg gcgatttctt tcgtgctggc
     2341 gccggcgcgc tcggcgaggt ctttgatttc gtcggcgacg accgcgaagc ctttgccgtg
     2401 ctcgccggcc tgagccgcga tgatggcggc gttcaacgcg agcaggttgg tctgctcggc
     2461 gacgtcgtcg atcacgttca agatctgccc gatggcatcg atgcgggagc cgaggttcga
     2521 gatcacggcc accgcttcct ggctgctctc cttgatgcgg tagatctcgc cgatggtctt
     2581 gaggatggct tccgcgccgc gttcggcgtc ttgcgccacc tcttcggaga ggcgcgcggt
     2641 ctcgttggcg ttcgactgaa cctggtcgat ggagacgtcc atctcgttca tgctcgagct
     2701 ggtttcctcg gcggtgagcg agagcgcgtc gacgttcttg gcgacctcct tgatggagta
     2761 ggccatctct tcgatcgatg cgaccgtttc acgcacgctc gaggcgagct cgacgatgtt
     2821 gccggccacc tgctcgttg
//
LOCUS       DASTKS010000542         7332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_85173,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000542 DASTKS010000000
VERSION     DASTKS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85173"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFQ35_11215"
     CDS             <1..299
                     /locus_tag="VFQ35_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9931252.1"
                     /translation="RDATISPGDLPFAKKPTGTQEPPPEVSTNGPSLDPLVDLPYREA
                     KDRAVHAFESSYFNALLQRAGGNVSEAARQAGLDRSNFRRAAKRAGVRMRDEGG"
     gene            296..1690
                     /locus_tag="VFQ35_11220"
     CDS             296..1690
                     /locus_tag="VFQ35_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9931253.1"
                     /translation="MTQSFVAKTCLFAALALFSHSVNADPVTDPVEERPARAFGAQIY
                     GLRRCLELAEQNYPRLHESRWRLAQKQAQYSQAKTAPYTDFTFTAGLAYAPRVIGTAL
                     YSPSSDVPITTDMGLAWQFGVSGVVPLWTFGKITNLWDAAEAQVKVGEGEVQKERNEL
                     RLAVRRAFYGVLLSRDALLLVREALERVDKYSNGLEEKVAAGDADEIQLLKLKIQRED
                     LLARESEARKQEAIALAGLKFLTGLSNGVNVPDRPLKRLEHHLGPLAHYLSAARIYRP
                     EINMARAGVLAREAQVRSERAKFFPDLGLALNASVINAPLVTDQRNPFVRDIGHLQSF
                     GAALALRYKLDFLPQAARYSQAQAQLEEQRATERYALGGVGVEVEQAFREAEDAERRL
                     DAFSRAVGYAKRWLIQVQQGIDVGTFEDGDVVDPAKEYALRRFSQMSATFDYNVAIAR
                     LAQTTGWDSIAADD"
     gene            complement(1817..3613)
                     /locus_tag="VFQ35_11225"
     CDS             complement(1817..3613)
                     /locus_tag="VFQ35_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET9931254.1"
                     /translation="MSARKKQKPPRKTSRPSRKPSPRAARPAKKRDARQGTKGRSAAG
                     GKARAEAKAPPRAKPKAAKKDQAAAKAKEKERLAKEKEQAAKAKEKERLAKEKEKERD
                     RLAKEKEQAAKAKEKEKEQAAKAKEKERLAKEKEKERERLAKEKEKERLAKEKEKEAE
                     KERIRKEREAEKERIRKEREEERARKIAEREAEREEARRQKEAERARREAEREAYRRA
                     KEAERERLRAEKEAARRALEGKVARASKRAQRGDGMGRGAGTTRVYRPDAIPNQSGTT
                     RRVGEIPLIGVARVVVNPPPAPVESLEQPSPLPPPPPEGSSPEGGAPGSHPPGAESKP
                     TPPPPPTLSVEERWARINQRLEATDTAFQREYRESFDMSWIYHDSALEGVVYTFQELK
                     TAIDPAVTVVADSSLQPVCEEIRRNRQAIELVRELAEKRRVPLNVDIIKKLYVTLHPE
                     EGDVKTVKYRKDIPQHRLYFHEYAPPDKIAYKVRQVVDWLNGPEPKKMKNAVRVAARV
                     HYDLLRSFPFQSDSGKVSRLLMNIILMRAGYPPAIIHSTERQKYYEALKGQLPIIIGM
                     TNDAITNALLSIEKRLEEYDARGRPGSAAPTA"
     gene            complement(3879..4724)
                     /gene="map"
                     /locus_tag="VFQ35_11230"
     CDS             complement(3879..4724)
                     /gene="map"
                     /locus_tag="VFQ35_11230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9931255.1"
                     /translation="MDMYLRSSTVEIKSPREIEAMRTVGRLAADTLNLVGSLVRPGVT
                     TEAINEFVHQDTLKKGARPAPLNYRGGGKGPPFPKSVCTSINEVVCHGIPGPRALREG
                     DIVNIDVTHIYGGFHGDTSATFYVGQKLSNEAKLVTEVARRCLELGIAQVRPGGRLGD
                     IGAAIQEFAEAHGCSVVRDFVGHGIGRRFHDEPKVTHYGQWGKGLRLRPGMTFTIEPM
                     INVGSWEVEMLDDDWTAVTADGSLSAQFEHTVLVTETGVEILTARDRSLENSEIFPDF
                     FKPGA"
     gene            complement(4776..5345)
                     /locus_tag="VFQ35_11235"
     CDS             complement(4776..5345)
                     /locus_tag="VFQ35_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931256.1"
                     /translation="MRGKIQLQGSYRLLVSFLLALGLAGAPLACGSKTNPSAVRALPE
                     YDAEQATLFGDVFRPELFGLGSERSVGVDGLLRDRATVADTVVAARVVTVSRETRGAA
                     RGYSVVLAPTENALVGPTPSGQLTFEIVETSPVFGWLEGASGAWGGTRMLLFLRQFRD
                     GTHFFGCADTEDVRTVVASAPRRRANPAP"
     gene            5500..6315
                     /locus_tag="VFQ35_11240"
     CDS             5500..6315
                     /locus_tag="VFQ35_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931257.1"
                     /translation="MKYSRFAAVVALLLGASLMGEAERPAFAKSPPAGATGNIEKSMQ
                     ISPKGIRWGMSLADISKLYEKVIEEEMKPQFRKAQPGMELDELTELLKDRQGALRRSK
                     IEFGTTPTGVDQSPLKGEYSYNNGESLAFMTLASGTKRNFFFFNDRLWKIYDEHKLKE
                     GGNLGKNFQEAVKALSERFGVPPKLVPADYDKGRPFDEAEWRDPDKLIRAVDRGNTLG
                     VVYVDRKVQEDLPRYRKNKTFESDEPLDKDVLNVTKKTEPEKKPEKETKGKKK"
     gene            complement(6418..6684)
                     /locus_tag="VFQ35_11245"
     CDS             complement(6418..6684)
                     /locus_tag="VFQ35_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9931258.1"
                     /translation="MSVPREALLRVVRDVIAPLVRADQGELYLVGLSDERVSLHLGGR
                     YSGCPGNTLARRRVIEPALHAVAPDAEIIITSGALIPAQAERLT"
     gene            6963..7205
                     /gene="rpsP"
                     /locus_tag="VFQ35_11250"
     CDS             6963..7205
                     /gene="rpsP"
                     /locus_tag="VFQ35_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9931259.1"
                     /translation="MAVHIRLSRHGTKKSAFYRIVVTDHRSPRDGRFIENIGVFDPAG
                     RFEIDRDRLAHWKSKGAQPSHTLDRLLKKNPAAPAA"
BASE COUNT         1203 a         2348 c         2358 g         1423 t
ORIGIN      
        1 gtcgcgacgc aacgatatcg ccgggcgatt tgcctttcgc caagaagccg accggcacgc
       61 aggagccgcc accggaggtc tcgaccaacg ggccgagctt ggatccgtta gtcgatttgc
      121 cctaccgcga ggccaaggat cgagccgtgc acgcgttcga gagcagctat ttcaacgcac
      181 tattgcagcg cgcgggcggt aatgtctcgg aggcggcgcg ccaggccggc ctcgaccggt
      241 cgaatttcag gcgtgcggcg aagcgagctg gggtcagaat gcgcgacgaa ggcggatgac
      301 tcagagcttc gttgccaaga cctgtctttt tgccgcactc gctctcttct ctcattcggt
      361 caacgccgat ccggtgaccg atccggtcga ggagcgacct gcgcgagcgt tcggcgcgca
      421 gatctatggc ttgcgccgtt gcctcgagct cgccgagcaa aactacccga ggcttcacga
      481 gtcgcgctgg cggctcgctc agaagcaggc tcagtactcg caggccaaga cggcgcccta
      541 caccgatttc acgttcacgg cggggctcgc ttacgcgccg agggtcatcg gaacggccct
      601 ctacagcccg tcgagcgacg tgcccatcac cacggacatg ggcctcgcat ggcagttcgg
      661 ggtttcgggt gttgtgcccc tctggacctt cggcaagatc acgaacctgt gggacgccgc
      721 cgaagcgcag gtaaaggttg gcgaaggtga ggtgcagaag gagcgcaatg agctgcgcct
      781 cgccgttcga cgggcattct acggagtgct gctctcgcgc gatgcgctgc tactggttcg
      841 cgaggctctc gaacgcgtcg acaagtacag caacggtctc gaagagaagg tggcagccgg
      901 cgacgccgac gaaattcagc ttttgaagct gaagattcaa cgggaggacc tgctcgcccg
      961 cgagagcgaa gcgcggaagc aagaggcgat cgcccttgcc gggctgaagt ttctgactgg
     1021 gctctccaac ggcgtcaacg tgcccgatcg gcccttgaag cggctcgaac accaccttgg
     1081 gccgctcgcg cactatctct cggcagcgcg aatctaccga cccgagatca acatggcgcg
     1141 cgccggggtg ctcgcgcgcg aagcgcaggt ccgctccgaa cgcgccaagt tcttccccga
     1201 tctcggcctc gctttgaacg cgagcgtcat caacgctcct ctcgtgaccg accagcgaaa
     1261 cccgttcgtg cgcgacatcg gtcacttgca gtcgttcggt gccgcgctcg ccctgcgcta
     1321 caagctcgac tttcttccgc aagcggcgcg gtacagccaa gcccaagcgc agctcgagga
     1381 gcagcgcgcg acggagcgct acgctctcgg cggcgtcgga gtcgaggtcg agcaagcgtt
     1441 tcgcgaagcg gaagacgccg agcgccgcct cgatgcgttt tcacgcgccg tcggttacgc
     1501 gaagcgctgg ctcatccagg tgcaacaggg catcgacgtt gggacgttcg aagacggcga
     1561 cgtcgtcgac ccggcgaagg aatatgcgct tcgtcgcttc tcgcagatgt ccgcgacgtt
     1621 cgactacaac gtggccatcg cgcggctcgc gcagaccacc ggctgggaca gcatcgccgc
     1681 ggacgactga gcgcgcgcgt atccatcgcg ggcggcgcgg ggagggcgcc caaaacctgc
     1741 cgcgagggtg ccgcggacgt tcggcggctc cctcgtgtcg tcgtcggcgc tcctcgtaaa
     1801 acgccggtcg agcggactac gcggtcgggg cggcgctgcc ggggcggcct cgcgcgtcgt
     1861 actcttccaa gcgcttttcg atgctgagca gcgcattggt gatggcgtcg ttggtcatcc
     1921 cgatgatgat gggcagctgg cccttcaacg cctcgtagta cttctggcgc tcggtcgagt
     1981 gaatgatcgc cggcggatag cccgcgcgca tcaagatgat gttcatcaag aggcgcgaaa
     2041 ccttcccgct gtcgctctgg aaggggaagc tgcggaggag atcgtagtga acgcgcgcgg
     2101 cgacccgcac cgcgttcttc atcttcttcg gctcggggcc gttcagccaa tcgacgacct
     2161 gacgcacctt gtaggcgatc ttgtcgggcg gcgcgtactc gtggaagtag agacggtgct
     2221 gggggatatc tttgcggtac ttgaccgttt tgacatcgcc ctcttccggg tgaagcgtca
     2281 cgtagagctt cttgatgatg tcgacgttca gcgggacccg ccgtttttcg gcgagctcac
     2341 gcacgagctc gatggcttgc cggtttcgcc gaatctcttc gcagaccggc tgcaagctcg
     2401 agtcggcgac gaccgtcacg gccggatcga tcgccgtctt tagctcctgg aacgtgtaca
     2461 cgacgccttc gagcgcgctg tcgtggtaaa tccacgacat atcgaagctc tctcggtact
     2521 cccgctggaa agcggtgtcc gtcgcctcga gtcgctggtt gatgcgcgcc caacgctcct
     2581 cgacgctcaa cgtcgggggc ggtggaggag tcggtttgga ttcggcaccg ggtgggtggg
     2641 aacccggcgc accgccttcg gggctgctgc cttcgggcgg gggaggcggg agcggtgaag
     2701 gctgctcgag cgattccacc ggcgccggcg gcgggttcac gaccacgcgt gcgacgccga
     2761 tgagcgggat ttcaccgaca cgacgggtcg tcccggattg gttcggaatc gcgtccgggc
     2821 gatagacacg ggtcgtcccg gcgccccggc ccatgccgtc gccgcgttgc gctcgtttgc
     2881 tcgctcgggc cactttgcct tcgagcgcgc ggcgagctgc ttccttctcg gcgcggaggc
     2941 gctctcgctc ggcctctttg gcgcggcggt aggcctcacg ctcggcttct cggcgagcgc
     3001 gctctgcctc tttctggcga cgagcttctt cgcgctcggc ctcgcgctcc gcgatcttgc
     3061 gggcgcgctc ttcctcgcgc tccttgcgaa tgcgctcttt ctcggcctcg cgctccttgc
     3121 gaatgcgctc tttctcggcc tctttttcct tctctttggc gaggcgttcc ttctcttttt
     3181 ccttggcgag gcgctcgcgc tctttttctt tctctttagc gaggcgctct ttttccttcg
     3241 ccttggcggc ctgctctttc tctttttcct tcgccttggc ggcctgctct ttttccttgg
     3301 cgaggcggtc gcgctctttt tctttctctt tggcgaggcg ctctttttct ttcgccttgg
     3361 cggcctgctc tttctctttg gcgaggcgct ctttttcttt cgccttggcg gcggcttgat
     3421 ccttcttcgc cgctttgggt ttggcgcgtg ggggcgcctt ggcttcggct cgcgctttac
     3481 cccccgctgc agaacggccc ttcgtcccct gacgcgcgtc tcgcttcttg gccggccgag
     3541 cggcgcgagg gctcggcttt cggctggggc gagacgtttt tcgcggcggc ttttgcttct
     3601 ttcgagcgga catcgacgaa aacagcgagt ggcgggcccc tacacctgta ataacccgac
     3661 ctgtcgtttt ttggcttgac agcggcagga gaccgcggtc gccgtgggcc atggccccgg
     3721 cgcaacgtag tcgtcccgcg tgcggtccgt caagccgagt gtgatgcgga cgcgtgagtt
     3781 catgtttgct gtgacgttgc ggtcgcgccg gcactcgcga gcgcagctcc gcacacgcct
     3841 ttcacgcgtc cggccgaggc gaccctgcgc gaccgacttc aagctccggg cttgaagaag
     3901 tcggggaaaa tttcgctgtt ttccagcgag cgatcccgcg cggtgaggat ttcgacgccc
     3961 gtctcggtca cgagtaccgt gtgctcgaat tgcgccgata gagatccatc agccgtgacc
     4021 gccgtccagt catcgtcgag catttcgacc tcccaggagc cgacgttgat catcggttcg
     4081 atggtgaacg tcatgcccgg tcgcagacgt aggccctttc cccactgccc gtagtgcgtc
     4141 accttgggct cgtcgtggaa gcgccggccg atgccatgac cgacgaagtc gcggaccacc
     4201 gagcaaccgt gcgcttccgc gaactcctgg atcgccgcgc cgatgtcgcc gagtcggcca
     4261 cctggcctaa cttgtgcgat tccgagctcc aagcagcgtc gagcgacctc ggtcacgagc
     4321 ttcgcctcgt tgctcagctt ttgaccgacg taaaacgtcg ccgaggtatc gccatggaag
     4381 ccgccataga tgtgcgtgac gtcgatgtta acgatatcgc cttccctgag agcgcgcggc
     4441 ccgggaatgc cgtggcaaac gacctcgttg atggacgtgc agacgctctt cggaaaagga
     4501 gggccctttc ccccgccgcg gtagttgagc ggagccgggc gagctccctt tttgagggtg
     4561 tcttggtgca cgaattcgtt gatggcctcg gtcgtgaccc cggggcgcac caggctaccg
     4621 acgaggttca acgtatctgc cgccaagcgc ccaacggtcc gcatggcctc gatttcgcga
     4681 ggtgacttga tttcgacggt gctactgcga aggtacatat ccatcggctg tcctgagggc
     4741 gctaccgcgt agcgtggatc tcccgggcgc aaggttcaag gggcgggatt ggcgcggcga
     4801 cgtggcgcgc ttgcgaccac ggttcgcacg tcttccgtat cggcgcagcc gaaaaagtgc
     4861 gtcccatcac ggaattgccg aaggaagagg agcatccgag ttcctcccca cgcgccgctg
     4921 gcgccttcga gccacccaaa caccgggctc gtttcgacga tctcgaacgt caactgtccg
     4981 ctcggcgtcg gaccaacgag tgcgttttcc gtcggtgcga gcacgaccga atagcctcgc
     5041 gccgcgccac gcgtttcgcg gctcaccgtg acgacgcgtg ccgcaaccac cgtatcggcg
     5101 acggtcgcgc gatcacggag aaggccatcg actccgaccg aacgttcgct gccgagcccg
     5161 aaaagttcag ggcgaaacac gtccccaaag agcgtggcct gttctgcgtc atactctggc
     5221 agcgctcgca ccgcgctcgg atttgtcttc gagccgcagg cgagcggcgc cccagcgagt
     5281 cccaatgcga gaaggaacga aacgaggagg cgatagctgc cttgaagctg aatttttccg
     5341 cgcatcggcg agcaacctcg tgggaacgag gcgggccggc gacgtatgat taaagcgcca
     5401 gcttcgaccg tccaaagggt tatgttggct tgagctgttc ggcgtcgagg tgtactttcc
     5461 cggcgacctt tcggcacggt ttttctggag gattcttcga tgaagtattc gcggttcgcg
     5521 gcggtggtag ctcttctgct cggcgcttcg ctcatgggcg aggccgaacg gcccgccttt
     5581 gccaagtcac ctcccgccgg tgccacggga aatatcgaaa agagcatgca gatctcccca
     5641 aagggcatcc gttggggaat gtcgctcgcc gacatctcga agctctatga aaaggtcatc
     5701 gaagaggaga tgaagcctca gtttcgcaaa gcacagccgg gcatggagct cgacgagctg
     5761 accgagctcc tgaaggatcg ccaaggggcc ttgcgccgca gcaaaatcga gttcggcacg
     5821 accccgaccg gcgtcgacca gtctccgctc aagggtgagt acagctacaa caacggcgag
     5881 agcctcgcgt ttatgacgct cgcgagcggc accaagcgga acttcttctt cttcaacgac
     5941 cggctctgga aaatctacga cgagcacaag ctgaaggagg gcggcaacct ggggaagaac
     6001 ttccaggagg ccgtgaaggc gctttccgag cgcttcggtg tgcccccaaa gctcgtcccc
     6061 gccgactacg acaagggccg gccattcgac gaggctgagt ggcgtgaccc ggacaagctc
     6121 attcgcgccg tcgatcgggg caacacgctc ggcgtcgtgt acgtcgaccg caaggtgcag
     6181 gaagatctgc cgcggtaccg caagaacaag acctttgaaa gcgacgagcc gctcgataag
     6241 gacgtcttga acgtgaccaa gaagacggaa cccgagaaga agcccgagaa ggaaaccaag
     6301 ggcaagaaga agtagggtgg gcccgggtcg ggcaggtgag gcgtcgcgcc gcctgcccgc
     6361 gcccgcgcgg cgcgaccccg ctgctgccgt ggctgcgctc cgaacttgga gcggagctta
     6421 cgtgaggcgt tcagcttgcg cggggatcag ggcgccgctc gtgatgatga tttccgcgtc
     6481 gggggccacc gcgtgcagcg ccggctcgat cacgcgacgg cgggcgaggg tattgccggg
     6541 gcaccctgag tagcggcccc cgaggtgaag tgacacgcgc tcgtcgctca gcccgaccag
     6601 gtaaagctcc ccctgatcgg cgcgaacgag cggcgcaatc acatcgcgaa cgactcgcag
     6661 cagtgcctcg cgcggaaccg acacgtgaag tgaccttaac acgcgccttt gcgagcgcag
     6721 ccctcctcgg cacccgcccc aaatttccgc cgcgaggccg ggactggatg cggtgggggg
     6781 ctcgggtttc gggaattcgg gtgctgttct gccaaagtgg cttgacgcac acgaaccgac
     6841 tctcctagtt tccgcgcccg gtccgaccgg cgggctttga cccggccgtt cggctctggc
     6901 gcccggagca tcgggtgcta gggttcgccc ccgcccggta ttgttaggag attcgagagt
     6961 tcatggccgt tcacatccgc ctttcccgtc acggcaccaa gaagtcggct ttctaccgca
     7021 tcgtggtgac cgaccatcgc agccctcgcg acggccgctt catcgagaac atcggcgtgt
     7081 tcgaccccgc cggtcggttc gagatcgatc gcgaccgcct cgcccattgg aagagcaagg
     7141 gtgcgcaacc ctcgcacacg ctcgaccgct tgctgaagaa gaaccccgct gctccggcgg
     7201 catgacgcct tcgcgcggct gcgctcgcag cgtggccgcg ctcaacggct gcttcggttt
     7261 gaagaggtaa gatggccctg aaggatttga tttccacgat tgcgcaggcg ctcgtcgacg
     7321 atcccgatgg tg
//
LOCUS       DASTKS010000543         3744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_753628, whole genome shotgun sequence.
ACCESSION   DASTKS010000543 DASTKS010000000
VERSION     DASTKS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753628"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VFQ35_11255"
     CDS             complement(<1..1412)
                     /locus_tag="VFQ35_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931260.1"
                     /translation="MDGLFIVRGTTVLLGLIACGIIIWRHERAFALLRKFFREPTSPI
                     NLAIVRVIVFYTLFESATKSEANFLASLPRGFQSLPRGWVWLADTGLFDSRVVHVAII
                     ALIVSTALAMVGALTPVATVVASACAILVFGVENFYFKIGHSLHVPTLSACVLAASPA
                     GDALSVDALVRRWRKKPRFEPSTAYTVPVRFCWLLVGTIYLFPGLWKLWETGDQWFDG
                     SKLSIEMFDKWAQLPDFAPTYRFDRAHTLLMFLGSWTIVIEVGIFFMIFARATRVAAA
                     LFASSFHIGVGLTLGIWFSPFVPLILLADIPEIFELRAFRPLLRLGEKARARLSRFFV
                     DRPERPTTLRNPRWTAASMLVGSFWLAGMFIAGLGPVDTWPIAIYPRFADRKSRPTST
                     GMTLGFVHVRRDGSEHELPNILDVLGDSASAFRVARTVIRARQRKDYRTLTRFEELFV
                     RCAERVMGPLQQGEQLRVVY"
     gene            2145..3620
                     /locus_tag="VFQ35_11260"
     CDS             2145..3620
                     /locus_tag="VFQ35_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931261.1"
                     /translation="MAWRTLSCLVLLAACSQNDSDAGGAGGQAGALSVRGGSAGNAAS
                     SGGISAGGTAGQATAAGGKAQAGDGSARGGVGNAMGGKGAAGGSASAGRGGASALGGS
                     GGMSAMSGGTSATCDASGGRGGQAGADVGMCVGRWKVQHLEDFGSTALPATAWSPDPV
                     PDDGPFADEGVYFTRDDISAPNAYRISQPFGEASWLTLESYTRDSTRKLADLASVVAD
                     PAGGTNKVLRVRSPQHTDATVVRSSAELPSRYRISLRVGYASFGDGVSDLNGYDSGDE
                     TAEPWLEDSAVSENGFYWLTILDSVPRPHNNVWIHHHRKVCIDSDNTTDGWMNVWNGS
                     SCEESGAHPIMMFALDGTSSGSPTSGKSFLSYRGGKWSSGICAVDAYRSERWYRVSIE
                     RFDDRFTLSISGDFTNGGNTTYTSTIDAVASCVFHYNQTPLPNGSRCVDTSFYPEVGS
                     SYPNWPSGATYPDYFMFGEPHENFYEGTVYYDDVQLETWEG"
BASE COUNT          717 a         1126 c         1261 g          640 t
ORIGIN      
        1 tagtacacga cacggagctg ctcaccttgt tggagcggac ccatcacccg ctccgcgcag
       61 cggacgaaca gctcttcgaa acgggtgagg gtgcgatagt ccttgcgctg cctcgctcgg
      121 atgacggtgc gagctacgcg aaacgccgag gcgctgtcgc cgagcacatc gaggatgttc
      181 ggcagctcgt gctccgagcc atcgcgccga acgtggacga agccgagcgt catcccggtg
      241 ctggtcggcc tcgacttacg gtccgcgaag cgcggataga tcgcgatggg ccacgtgtcg
      301 actggaccga gcccagcgat gaacataccc gcgagccaaa aactgccgac gagcatcgac
      361 gccgcggtcc agcgcgggtt ccgcagcgta gtcggacgct cgggtctgtc gacgaagaac
      421 cgactgagcc gagccctcgc cttttcgccg aggcggagca gcggcctgaa agctcgaagc
      481 tcgaagattt cgggtatgtc tgcgagcaag atgagcggca cgaacgggct gaaccaaatt
      541 ccgagggtga gcccgacgcc gatgtggaac gacgacgcga acaacgcggc tgcgacgcgg
      601 gtcgctctcg cgaagatcat gaagaagatg ccgacctcga tgacaatggt ccagctgccg
      661 aggaacatca gcagggtgtg cgcgcgatcg aagcggtaag tcggagcgaa atcgggtagc
      721 tgcgcccact tgtcgaacat ctcgatggag agcttggagc cgtcgaacca ctggtcaccg
      781 gtctcccaga gcttccacaa gccggggaag aggtagattg tgccgaccag caaccagcag
      841 aagcgcacgg ggaccgtgta cgccgtcgat ggttcgaagc gtggcttctt tcgccagcgc
      901 cggaccaacg cgtcgaccga gagcgcgtcg cccgccggac tcgcagcgag tacgcacgcc
      961 gacaacgtcg ggacgtgaag gctatgcccg attttgaaat agaaattctc gactccgaac
     1021 accagaatcg cgcacgcgct cgcgacgacc gttgcgaccg gcgtcagcgc gccgaccatc
     1081 gcgagcgccg tagagacgat gagcgcgatg atcgcgacgt gaacgacgcg cgagtcgaac
     1141 agtcccgtgt ctgctagcca aacccaccct cgcggcaagc tttggaaacc gcgtggcaac
     1201 gacgcgagga agttggcttc gctcttcgtc gcgctctcga acagcgtgta gaagacgatg
     1261 actcgaacga tcgccaaatt gattggactc gtcggttctc gaaagaattt ccgaaggaga
     1321 gcgaacgcgc gttcgtgtct ccaaatgatg attccgcacg cgatgagccc caggagcacc
     1381 gtcgtgcccc gcacgatgaa caacccgtcc atgacgcgcg taaactatcc cagaacgagc
     1441 cgccgcggga ttggtaatgg attcaggcca gcgagccatc gatattgggc ggggcgcgag
     1501 tcgggcgggt atcagcgtca gcgggcgcca tccaaggcgg ctatcggaat cgagaagaag
     1561 atcgcctcgg gttgggctca aagtcggttg tacgggcgac gttcaggatg cggctgacga
     1621 gaggtttagc gccgaggctt gctgggatga gcgacgcagc ggtgacccgg ccggcgcggt
     1681 ccacgtcgaa gtccaacgag acacgcggag cattggaggc ggcttcgtgg cttgggtcga
     1741 agcaccgttg aattgcgctc ctgcgcgatt ggcgcgcgct cgcttcggcg cgacacgacc
     1801 gcgaaacacc acggccagcg cgccgagagc ggggacttgc ccgcgccggt ccgagccagt
     1861 gctcgcgcgg cggtcagaag ccggtgctcg cgcggcggtc agaagctggt gctcgcgcgg
     1921 cggttagaag ccggtggtcg cgcggcgccc tcgggcgcta cggcggaatt cgacccgcgt
     1981 tccgcgcctc gcgttttcct ggctcgcgcc acgcgacgga ttttcttggt tctcgcgatg
     2041 ggcagaaacg gggcaacccc tcaaagcccg caattgctgg aacgttcact cttctcctgc
     2101 tcggagcagg tgacccgagg cggtgctcgc actaaaatga tgggatggct tggcgcacgc
     2161 tctcttgcct cgttcttctc gcggcgtgct cccaaaacga ctccgatgca ggcggggctg
     2221 gcggacaagc gggcgctctc tcagtgcgtg ggggaagcgc tggtaacgcg gcgtcctcag
     2281 gtggcatcag cgcgggcggc accgccgggc aagcgaccgc ggctggaggt aaagctcaag
     2341 ccggtgacgg cagtgctcga ggcggtgtcg gcaacgccat gggtggcaag ggggcggcgg
     2401 gcggctcggc gagcgccggg cgcggtggcg cgagcgcgct gggtgggagc ggcggcatga
     2461 gcgcgatgag cggaggcacg agcgcgacgt gcgatgcaag cggcggtcgc ggtggccaag
     2521 ccggcgccga cgttgggatg tgtgtcggac gttggaaggt ccagcacctc gaggacttcg
     2581 gttcgaccgc tttgcctgcg acggcgtggt cgccggatcc ggttcctgat gacgggcctt
     2641 ttgcagacga aggcgtctac tttacgcgcg acgacatttc ggcgccgaac gcataccgca
     2701 tcagtcagcc gtttggtgaa gcgagctggc tcactctcga atcgtatacg cgggacagca
     2761 cccgaaagct cgcagacctc gccagcgtgg tcgcagatcc ggccggcggc accaacaagg
     2821 tgctgcgcgt tcgctcaccg cagcacaccg atgcaaccgt cgttcgctcg agtgcggagc
     2881 tgccgagtcg ctatcgaatc agcctgcgcg ttggttacgc gagctttggg gacggcgttt
     2941 cggacctgaa tggctacgat agcggcgacg agacggcgga gccgtggctc gaggactcgg
     3001 ccgtgagcga aaatggcttc tattggctca cgattttgga ttcggtgccc cggccgcaca
     3061 acaacgtttg gattcatcat catcgtaagg tgtgcatcga ctctgacaac accactgatg
     3121 gttggatgaa cgtctggaac ggtagctcgt gcgaagagag tggtgcccat ccaatcatga
     3181 tgttcgcgct ggacggtacc tcgtccggct caccgacgtc gggtaagtcg tttctgtcgt
     3241 atcgcggcgg caagtggtcg agcgggatct gcgccgtgga tgcgtaccgc tccgaacgct
     3301 ggtaccgcgt gagcatcgag cgcttcgacg atcggtttac gctcagtatc tcgggtgact
     3361 tcacgaacgg aggcaacacg acgtacacga gcaccatcga cgccgtggca agctgcgtct
     3421 ttcactacaa ccagaccccg ttgccgaacg gatcgcgctg cgtcgatacg agcttttacc
     3481 ccgaagttgg ttcgagctat ccgaactggc cgagcggcgc gacgtacccc gactacttca
     3541 tgttcggaga gccccacgag aacttctacg agggcaccgt ctattacgac gacgttcagc
     3601 tcgagacgtg ggaagggtag tagtttacgc tcggttcgtg cctaaagggc gctaaggacc
     3661 tcgccgaatt tggcccgctg gcgacggctc atccattcac gggccctagg acctcgccga
     3721 atctcggctc ggcgggtatt ctgc
//
LOCUS       DASTKS010000544         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1647087, whole genome shotgun sequence.
ACCESSION   DASTKS010000544 DASTKS010000000
VERSION     DASTKS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647087"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFQ35_11265"
     CDS             <1..584
                     /locus_tag="VFQ35_11265"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931262.1"
                     /translation="LRHVHSTKDSDFRLERIAESHVSELAGKLGNLLQRTTALALKHR
                     ELRICRHATPSEDDELLIHAATRAFADVPAAVDDFALHEALSSIFALVGAANRYADAR
                     EPWALSRRLTTNGSDDCAGELGNVLWHLLEALRVVAVLVAPFLPRTAAQLCERIGVPR
                     RQLIDLENARFGSWSTYAPDGGPPLFPLEPRSG"
     gene            837..2093
                     /locus_tag="VFQ35_11270"
     CDS             837..2093
                     /locus_tag="VFQ35_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9931263.1"
                     /translation="MRTSRLARARRRALATTSSRRSLLSLILATACVTVACAGENDSS
                     YPKACSDVYDTSRVPTFELEMSAAELTGMEQDCAQKVKKYRPATFRYGSESADVMVRL
                     KGNWSFRCEKKQFVISFNEVNSDARFHGLRKIMLDAPWYDPSLLAERLGFSFMRRTGN
                     YSSCSNSARLMFNGQYYGAYTSSERIDKEYLQRHFSKADAEGNLYEGGKELKTNESEN
                     DTSRRDALFAAKDVASIAALADLDQAVKEWAALAMLPDPDSYWAGVEINYYLYDHPSR
                     GFVWLPVDMDLTMKQGVTNANTSSIKVGVQEEFVRADPFTYQNSAWGREPIYRTVLSD
                     EAWCNRFLDALRAARDAYDVQAMSKDLDTWAAQIADAVDKDPHKPYTMQTHLEGVATL
                     KAFMPQRLAFVDTWLSTARCPVTKWP"
BASE COUNT          451 a          743 c          689 g          383 t
ORIGIN      
        1 tgttgcgcca cgtgcactcc acgaaagaca gcgacttccg actcgagcgc atcgccgaat
       61 cccacgtcag cgagctcgct ggaaagctcg ggaatctgct ccaacgcacc accgcgctgg
      121 cgctcaagca ccgcgagctc cgtatctgca ggcacgcgac cccgagcgaa gacgacgagc
      181 tcctcattca tgccgccacc cgcgcgttcg ccgacgtgcc agcggcggtg gacgacttcg
      241 ccttgcacga ggcgctttcg agcatcttcg cgctggtcgg cgccgccaac cgatacgccg
      301 acgcgaggga gccctgggct ctttcccgcc gattgaccac gaacggcagc gacgactgtg
      361 caggagagct cggcaacgtg ctttggcacc tgctcgaagc gctgcgcgtc gtggcggtgt
      421 tggtcgcgcc gttcttgccg cgcaccgcgg cgcagttatg cgagcgcatc ggggttcctc
      481 gacgccagct catcgacctc gagaatgcac gtttcggctc ctggtcaacg tacgcgccag
      541 acggcggacc accgcttttc ccgctcgaac cgcggtcggg ttagggcggc ccttaagagc
      601 cagtccaaaa tcctggaccg agcccggtaa agaaagcagc tcgcagttct ggcgggactc
      661 catcgaagcc cgcgcacggc gtcactctgt ttggaacgca ccgccgaggt agtagcgaag
      721 cacacgtgct gccctgtcgg cgcaggcgcc acgagccgac agagcgcaca ccttaggcca
      781 ctcttcgacg cccgttcgta aacgagccgc gccgcacgat gctacgctga cgcggaatgc
      841 gcacatctcg gctcgcgcgc gcgaggcgcc gcgctctcgc aaccacgtcg tctcggcgtt
      901 cgctgctctc gctcattttg gcgaccgcgt gcgtgaccgt ggcgtgcgct ggagaaaacg
      961 actcgagcta tcccaaagcg tgcagtgacg tgtacgacac gagccgcgtg ccgacgttcg
     1021 agctcgaaat gagcgcggcg gagctcactg gcatggagca ggactgcgct caaaaggtga
     1081 agaagtaccg ccccgcgact ttccgctatg ggagcgagag cgccgacgtg atggtgcgcc
     1141 tcaagggcaa ttggtccttc cgctgcgaga agaagcagtt cgtcatttcg ttcaacgagg
     1201 tgaactcgga tgcgcgcttc cacggcctcc gcaagatcat gttggacgca ccctggtacg
     1261 atccgtcgct cctcgccgag cgcctcggct tctccttcat gcgccgaacc gggaactact
     1321 cgtcgtgctc gaacagcgcg cgcctgatgt tcaacgggca gtactacggc gcgtacacga
     1381 gctcggagcg catcgacaag gagtacctgc agcgtcactt ctccaaggca gacgccgagg
     1441 ggaacctgta cgagggcgga aaagagctca aaaccaacga atccgagaat gacacgagcc
     1501 ggcgtgacgc gctcttcgcc gcgaaggacg tcgccagtat tgcagccctc gccgacctcg
     1561 accaagccgt gaaggagtgg gccgcgctcg ccatgctgcc cgaccccgac agctactggg
     1621 ccggcgtcga gatcaactac tacctgtacg accatccgtc gcgcggcttc gtgtggctgc
     1681 ccgtcgacat ggatctgacg atgaaacagg gagtgacgaa cgcgaacacg tcgagcatca
     1741 aggtcggggt gcaagaggag tttgtgcgcg ccgatccgtt cacttatcag aactccgctt
     1801 gggggagaga gccgatttat cgcaccgtgc tgtctgacga ggcgtggtgc aaccgcttcc
     1861 tcgacgcatt gagagccgcg cgcgacgctt acgacgtgca agcaatgtcc aaagacctcg
     1921 acacctgggc tgcgcaaatc gccgacgccg tcgacaagga tcctcacaag ccgtacacga
     1981 tgcaaacgca cctggaaggc gtcgcgaccc tgaaagcgtt catgccgcag cgcctcgcct
     2041 tcgtcgacac ctggctatcg accgcgcgtt gcccagttac gaaatggcct tagagcaatc
     2101 ctaaatccct cccgtccagg atttaggact ggctcttaga agtccaagtg cgcgtgcgct
     2161 actttacgcc gcgcgccgac aagaattgac cgaggcgtcg atcggcttcg ctgatatggt
     2221 gagcgagcca aaactcgagg aacttgacga ccatgggaag ggtcag
//
LOCUS       DASTKS010000545         6032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_532207, whole genome shotgun sequence.
ACCESSION   DASTKS010000545 DASTKS010000000
VERSION     DASTKS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532207"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFQ35_11275"
     CDS             complement(<1..693)
                     /locus_tag="VFQ35_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9931264.1"
                     /translation="MIEGKKVVVVMPAYNAAKTLERTVAEVPSLVDQIILVDDKSSDD
                     TVAISSRLGLHTVVHPMNRGYGGNQKTCYTQALRVGADIVVMVHPDYQYTPKLIPALA
                     HCIASGLYDVALGSRILGGRALKGGMPLYKYVANRLLTATENLLLSEKISEYHTGYRA
                     FSRRLLETLPLEENDDDFVFDNQMLVQAVHFGFSIAEVTCPTAYFPEASSINFARSVK
                     YGLGVLRAAVDHR"
     gene            819..4778
                     /locus_tag="VFQ35_11280"
     CDS             819..4778
                     /locus_tag="VFQ35_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931265.1"
                     /translation="MDTTRIWNAAGTRSVAAIAALLVTPLLALMFGGNAQSQPAEPAP
                     AHSAPPSPAPAAEPLEYCAGRLFEPLASISGSEVVTGGAGFQAGLEATLADVRKTFPG
                     PIQTLIATIADPIDAGLGYQFDTSLSALRLGVESHVASSRAHYFRDRAFIPWDDRSAP
                     PDKRKESEACRYTTPGLVVFRGSDASHPELFALLLVGETPTTGVHERALHRAVQIARA
                     FDPRAGTDTSTPLNVIGPTFSGSAHSLRLAFSQAEGLRHVRVITGSASGSALKKTLTG
                     ATAGPKLSFLSTTAPESAVLCSYLRFLYYFLGFQSGSLEPFTGVPRAIPGVALLHESG
                     TEFGTGHDDTRSGGCRLRPEVDIAFPVHVSRLRDAYEEMDTQQKSGTSTERLARATTL
                     DISLRERNLPLTLGAEPSPKTTFAEDVALSNVLTAIAREGVRHVGIQATDVADAIFLA
                     RKIRDVSPDVRLAFFASDVLLLHPAFRRDLGGSLVVSPYPFLGSTDFAQVGAPETHSH
                     FGFENSASEGIFNATLAARGATVPELRDFRFTDIEALSLWVSAIGPNGLVPIALGMTD
                     DPDGTIFPGTEKPRKPGAQDEQTWRKLARAELRLAQNPHIPRLFQFAYVLLWLAFCID
                     QVMQRRTLETFSWLPVPGRMGRREDRQADLAIVRTKWSLYAAIRTFVLAFGIGYMTLV
                     YALPSALRRHGGERVVLALLSLLALGAVYRALVKAWAFGSDYVALGRCVGGFSPLTWV
                     SLRFRPRASQVPPESIRGTPLSDEQSQDSALEGEKPVSSLGWDRWSAPLGFARPEGRV
                     PAAQTSFAQLRLLANLALAVALAFSAGEVVEFFGRALTVFEQGHPVQGTTLLTLRSLP
                     LLNTVSPAAPTLICVAIVYIWAVGRMARIRVAHGLSRISPDDGVADVATTPIRVVLYP
                     YHGEGQRADESFTRIERRAINAVIRPITGPNYCVALLIIIFIPVVLFTLKPLSTLERP
                     LSTALLVSGLGLCTVLVGNTLLQLVQHWFSLELMLKRILQHPLGRSFDRVAGFVRDST
                     DHQVSRSPHDLLRLGGCAVRFDELVRAGKRLDASGFALERRKRLESLALTVHSMRTIA
                     LSAATHTDLRDASNHEANLGREIIRAASEVMKLLCDAWEGRILRTPPAFRGKAVERTR
                     APEEDDASEPAESGPIDSTDDALSAEAHRYSKLERAWLREAEGFVATVVALLINRHVR
                     QFQYFLYTMTSSALLLLLAIVSYPFQPQRLLLTWIWVVVGSVVLAGLFVYIELDRNAL
                     LSRIAGTTPGHLTLNGSLLFRVLGWGIVPLLGVAAAQYPDFANTLFHWLEPFTRALR"
     gene            complement(4737..>6032)
                     /locus_tag="VFQ35_11285"
     CDS             complement(4737..>6032)
                     /locus_tag="VFQ35_11285"
                     /inference="COORDINATES: protein motif:HMM:NF039677.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HET9931266.1"
                     /translation="RAGDDEEFDAARFVTWLEVLQEAGDAFAAARVTELSLDFVTLAF
                     NELLLVLNVDSMIQELDGGGEEVDGIEKALSNCAYEELDEFQLIARRADGWDAVWSLV
                     LALDRDHRDFLRRVLERCCRASHAYIEENGGLQDVLSDEEMFESDARAERDDRRAAKG
                     HVAASDAKSFLGLAASGAEPRERDAVTRAYFRELEPSVAAPKGSVDRRRSGARIERLL
                     AALEVDESTARAPRLAGASEEARLPLSVALDELSEGALVARYREELAYLANVLIAGAA
                     LEERRFRPIEALEAAIAVTNLGMELAMDSTRLSAVEVLSATSADVLFRQGFAVLRRDV
                     VAPARANRASSSLGEDVPRLPDAGGLGFVATREQLARATRLAVPKESATKQTAPKQTA
                     TKQPSSKQPSSKKEPATKQRATKQRSSTKRAREGLEPVK"
BASE COUNT          961 a         2204 c         1787 g         1080 t
ORIGIN      
        1 ccgatggtcg acggccgcgc ggagcacgcc gagcccgtac ttgacgctac gggcgaagtt
       61 gatcgacgac gcttccggga agtacgcggt cgggcacgtc acctcggcga tgctgaaccc
      121 gaagtgcacc gcctgcacca gcatctggtt gtcgaaaacg aagtcgtcgt cgttctcttc
      181 gagcggcagc gtctcgagca ggcgccgcga gaacgcgcga tagccagtgt ggtactcgga
      241 gatcttctct gacaagagga gattctcggt cgcggtgagc aggcgattcg cgacgtactt
      301 gtagagaggc ataccgccct tcagcgcgcg cccacccaaa atgcgcgagc cgagcgcgac
      361 gtcgtaaagg ccgctcgcga tgcagtgcgc gagcgccgga atgagcttgg gcgtgtactg
      421 atagtcgggg tggaccatca ccacgatgtc agcccccacc cggagcgctt gcgtgtagca
      481 cgtcttttga ttgcccccgt acccgcggtt catcgggtga acgacggtgt gaagtcccag
      541 tcggctcgaa atggcgaccg tatcgtcgct cgatttgtcg tccaccagga tgatctggtc
      601 gacgagcgac ggaacctcgg caaccgtgcg ctctagagtt ttcgcggcgt tgtacgccgg
      661 catcactacg actacttttt tgccctcgat cacgcttttt cccttactac acacccttct
      721 tccgtgaaag ccgctccgca gagcaatgac tatcgccgcg ccgagctttc gggcagataa
      781 acgagggggg tcgcgggcta cgcggccgac ctagggaaat ggacactacg cgaatctgga
      841 acgcagccgg cacccgcagc gttgcggcca ttgcggccct cttggtcacg ccgcttctcg
      901 cgctgatgtt cggcgggaac gcgcagagcc agccggcgga gcctgcgcca gcgcattcgg
      961 ccccgccgag ccctgcccca gccgctgaac cgctagaata ctgcgccggg cgcctcttcg
     1021 agccgctggc gtctatctcg ggcagtgaag tagtcaccgg aggcgcaggt tttcaggcag
     1081 ggctcgaggc gacgctcgcg gacgtgcgga aaaccttccc cgggcccatt caaacgctga
     1141 tcgcgaccat cgcggatccg atagacgcag gcctcggcta ccagttcgat acctcgctgt
     1201 cggcgctgcg gctcggggtc gagtcgcacg tcgcttcgag ccgcgcgcac tacttccgcg
     1261 atcgagcctt cataccgtgg gacgatcgct ccgcgcctcc cgacaaacgc aaagaatcag
     1321 aggcgtgtcg ctacacgacg cccgggctcg tcgtgttccg cggcagcgac gcgagccacc
     1381 cagagctgtt cgcgctgctg ctcgtcggag aaaccccgac gacgggcgtg cacgagagag
     1441 cactgcatcg cgccgtgcaa atcgcgcgcg ctttcgatcc gcgcgcgggg accgacacat
     1501 cgacgccgct caacgtgatc ggtcccacct tctcgggaag tgcgcactcg ctgcgcctcg
     1561 ccttctcgca ggcagaagga cttcggcacg tccgcgtcat cactgggtcc gcgagcggga
     1621 gcgcgctgaa gaagacgctg accggcgcga cggcgggacc caagctctct tttctttcga
     1681 ccaccgcgcc cgagagcgcc gtcctctgca gctaccttcg ctttctctac tacttcctcg
     1741 gctttcagag cggctcgctc gagccattca ccggggtgcc gcgagcgatc cccggggtag
     1801 cgctgctgca cgagtccggc accgagttcg gcaccggcca cgacgacacg cggagcggcg
     1861 gctgccggct gcggcccgaa gtggacatcg ccttcccagt gcatgtctct cgcctgcgcg
     1921 acgcctacga ggagatggac acccagcaaa agagcgggac ttcgaccgag cgcttggcgc
     1981 gagccacgac cctggacatc tcgctccgcg aacgcaactt gccgctgacg ctgggcgccg
     2041 agccgtcccc gaagacgacg ttcgccgaag acgtcgcgct ctcgaacgtg ctcaccgcca
     2101 tcgctcgcga gggcgtgcga cacgtcggca ttcaggcgac cgacgtcgcc gacgcaatct
     2161 tcctcgctcg aaagatccgc gacgtctctc ccgacgtgcg cttggcattc ttcgcctccg
     2221 acgtactgct acttcacccc gcttttcggc gcgacctcgg cggctcgctg gtcgtgagcc
     2281 cctacccgtt cctgggatcc accgacttcg cgcaagtcgg cgcgccggag acccactcgc
     2341 acttcggctt cgaaaactcg gcgagtgaag gcatcttcaa cgcgacgctc gcggcgcgcg
     2401 gcgctaccgt ccccgagctt cgcgactttc gcttcaccga catcgaagcg ctctcgctct
     2461 gggtctccgc gatcggcccg aacggcctcg ttccgatcgc gctcggcatg accgacgacc
     2521 cggatggaac catcttcccc ggcaccgaaa agccgcgaaa gccaggtgcc caagacgaac
     2581 agacctggcg caaactcgcc cgcgcggagc ttcgcctcgc gcagaacccg cacatcccgc
     2641 gactgttcca gttcgcatac gtgctgctct ggctcgcctt ctgcatcgat caggtcatgc
     2701 aaaggcgcac cctcgagacc ttctcgtggc ttccggtacc gggccgcatg ggtcgtcgcg
     2761 aagatcggca agccgacctc gccatcgtgc ggacgaagtg gtcgctctac gccgccattc
     2821 gaacgttcgt gctcgcattc ggcatcggct acatgacgct ggtctacgcg ctcccgtccg
     2881 ccctgcggag gcacggcggc gagcgcgtcg tgctcgcgct gctctcgctg ctggcgctcg
     2941 gagccgtgta ccgagccctc gtcaaagcct gggcgttcgg ctcggattac gtggcgctcg
     3001 gccgctgtgt cggcggcttc tctcctctca cgtgggtcag cttgcggttt cggccccgcg
     3061 cgtcgcaggt gccacccgag agcattcgag gcacgcccct ctccgacgag caaagccaag
     3121 attcggcgct cgagggagag aagcccgtga gctccttggg ctgggatcgc tggtcggcgc
     3181 cgctcggctt cgcccgaccc gaggggcgcg tcccggcggc tcagacttcg ttcgcacagc
     3241 ttcgcctgct cgcgaacctg gcgctcgcgg tcgcgctcgc gttcagcgcc ggtgaagtgg
     3301 tcgagttctt cggacgcgcg ctcaccgtgt tcgagcaagg ccacccagtg cagggcacga
     3361 ctctgctcac gctgcggagc ctaccgcttt tgaacaccgt ctcgccagcg gcgccgaccc
     3421 tcatctgcgt cgccatcgtc tacatttggg cggtggggcg catggcgcgc attcgggtcg
     3481 cgcacggcct ctctcgcatc tcgccggacg acggcgtggc cgacgtcgcc accaccccga
     3541 ttcgcgtcgt cctctacccg tatcacggcg aaggacagcg tgccgacgag tcgttcacac
     3601 gcatcgagcg ccgggccatc aacgccgtga ttcgaccgat caccggaccg aactactgcg
     3661 tcgcgctgct gatcatcatc ttcatccccg tcgtgctgtt cacgctcaag ccgctgtcca
     3721 cgctcgagcg gccgctctcc accgcgctgc tcgtctccgg tctcggcctc tgcaccgtgc
     3781 tcgtcggcaa caccttgctg cagctcgtgc aacactggtt ctcgctcgag ctgatgttga
     3841 agcgcatcct gcagcatccg ctgggccgct ccttcgaccg cgtggcgggc ttcgtgcgcg
     3901 actcgacgga ccaccaagtc agccgctcgc cgcacgatct cctccgcctc ggtgggtgcg
     3961 cagtgcgctt cgacgagctc gtgcgcgccg gcaagcgcct cgacgcgagc ggcttcgcgc
     4021 tcgaacgccg caaaaggctc gagtcgctgg ccctcaccgt ccattcgatg cgaaccatcg
     4081 cgctctccgc cgccacccac accgatctgc gcgatgcctc gaatcacgag gcgaacctcg
     4141 gccgcgaaat cattcgcgcc gcgagcgaag tcatgaagct cctctgcgac gcatgggaag
     4201 gtcgcattct gcgcacgccg cccgcctttc ggggcaaggc ggtcgagcgc acccgcgcgc
     4261 ccgaagaaga cgacgcgagc gagccggccg aaagtgggcc catcgactcc accgacgacg
     4321 cgctctccgc cgaagcgcac cgttactcga agctcgagcg cgcctggctg cgtgaagccg
     4381 agggcttcgt cgccaccgtg gtcgcgctgc tcatcaaccg ccacgtgcga cagtttcagt
     4441 acttcctcta cacgatgacg agctcggcgc tcctgctact gctcgcgatc gtgagctacc
     4501 ccttccagcc tcaacgcctg ctccttacct ggatttgggt cgtcgtcggc tcggtcgttt
     4561 tggccgggct cttcgtttac atcgagctcg accgcaacgc gctcctgagc cgcatcgctg
     4621 gcacgacgcc cggccatctc acgttgaatg gcagcttgct cttccgcgtg ctcggctggg
     4681 gcatcgtgcc gctgctcggc gtcgccgcgg cgcaataccc cgacttcgcg aacacgctat
     4741 ttcactggct cgagcccttc acgcgcgcgc ttcgttgaac ttcgctgctt cgtcgctctc
     4801 tgcttcgtcg cgggctcttt ctttgacgat ggctgcttcg acgatggctg cttcgtcgcc
     4861 gtctgcttcg gcgctgtctg cttagtcgca gactccttcg gcaccgcgag cctcgtcgcc
     4921 cgtgcgagct gctctcgtgt cgcaacgaag ccaagcccgc ccgcgtccgg aaggcgcgga
     4981 acgtcttcgc ccaagctcga cgaagcccgg tttgcgcgcg ccggcgccac gacgtcgcgc
     5041 cgcagcaccg cgaacccctg ccgaaacaac acgtccgcgg aggttgcgct caatacctcg
     5101 accgcgctca atcgcgtcga atccatcgca agctccatcc ccaagttcgt cacggcgatc
     5161 gccgcctcga gcgcctcgat aggccgaaag cgccgctctt cgagcgcggc acccgcgatg
     5221 agcacgttcg ctagatacgc tagctcctcc cggtaacgag cgacgagcgc tccttccgaa
     5281 agttcgtcga gcgccacgct gagcggcagc ctcgcctcct cggacgcccc ggccagcctc
     5341 ggcgcgcgcg ccgtgctttc atccacttcg agcgctgcaa gcaggcgttc gatgcgtgct
     5401 ccactacgcc gccggtcgac gcttcccttc ggagccgcga cgctcggttc gagctcccga
     5461 aagtacgcgc gtgtcaccgc gtcccgttcg cgcggctccg caccgctcgc agccaaaccc
     5521 aagaagctct tcgcatcgct cgccgccacg tgccccttgg ccgcacgtcg atcgtccctc
     5581 tccgcgcgcg cgtcgctctc gaacatctcc tcgtcgctca gtacgtcctg tagcccgccg
     5641 ttctcttcga tgtacgcgtg gctcgcgcgg cagcaccgct ccaaaaccct ccgcaagaaa
     5701 tccctgtgat cgcgatccag cgccaacacg agcgaccaga ccgcgtccca accgtccgcc
     5761 ctgcgcgcga tcaactgaaa ctcgtcgagc tcctcgtacg cgcagttcga aagcgctttc
     5821 tcgatgccgt cgacctcttc gcctccaccg tcgagctcct ggatcatcga atcgacgttc
     5881 aacaccaaga gcaactcgtt gaacgccagc gtcacgaaat cgagcgacaa ctcggtgacg
     5941 cgagctgccg caaatgcatc ccccgcctcc tgcaacacct cgagccacgt cacgaagcgc
     6001 gctgcgtcga actcttcgtc gtcaccggct cg
//
LOCUS       DASTKS010000546         6518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1425449, whole genome shotgun sequence.
ACCESSION   DASTKS010000546 DASTKS010000000
VERSION     DASTKS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425449"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3091)
                     /locus_tag="VFQ35_11290"
     CDS             complement(<1..3091)
                     /locus_tag="VFQ35_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET9931267.1"
                     /translation="MLKFLVLASIRARGAVFATLFLLLGVGVWAARTLPIDALPDVST
                     VQVSVLTSASGLSPVEIERTVTMPIENALNGVPGAIDQRSVSRSGLSAVTVIFRETMD
                     IWFARQLVLERLRGVELPASAGAPELAPVSSGLGEIFQFVVRSDQHSAMQLRTLLDWE
                     IVPKLRNVPGVVEVNTMGGDLKQFQVVADRAKLKAQGLSLGDVVDALRAANMNAGGGY
                     IDRREESFTVRGQGMLSGPDEIGNVMVRTTPEGMPVLIHHIADVKVGAALRQGVITHN
                     GKGEAVTGVVMMLLGENSRNVVTAVAKRAAEIQTKLPPGVKIEVIYDRSDFVGRTLST
                     VMKNLAEGIAIVTIVLILFLGTWRGALAVVLGIPCAMSVALIGMHLFHVTGDLMSLGA
                     IDFGFLVDGPIVILEAVIAATAGKKLVGAARAGAYGEIAGAVARPVAFAVAIIMLVYI
                     PLLALEGVEGKMFRPMAVTMASALFGALIYAVLFFPAVLVVLVPPAAGHGPHWLERLT
                     ALYQRMLASIIRFRVVWVAGGVAVFGVSAWLFTSQGAEFVPRIFEGDAVVTIRRAPSI
                     SLEAARKLDLDAEKVLHSFPEVVTTLGMTGRAEVAVDPVGNDNTDLFVRLRPLGEWTS
                     AHDFDELSELFKTQIENKVPGTFVSVSQPIEDRTNELISGSRADVQIQIFGDQLDTLM
                     KLAQSVGNRVRKIQGTGDVRVERVLGAPTISAVADRGRLARYGVRVEDAFAVLAAARE
                     GVPVGPVYEDERRFELRVLEPPARPTADGIGDLFVTTRNSENVPLREVLSITEGEGPT
                     AIRRENRTRAVRVDVNLRGRDLVSWVSEAQAAVKDSVPLPTGYRVEWGGQFENFERAQ
                     ARLAIVVPVVIMLIFGMLLWMFQNLRLTIAVFVLVPLSLAGGMLGLVLRSMPFSLPAA
                     VGFIALGGIAVLNGVVMASEVRKRLDAGQLLDDAVTGGCGATLRAVLTTAAVAALGFL
                     PMATATSAGAEVQRPLATAVVLGMAISTLLTVFVLPGVLRIMLAGYRRPEAEEPEARA
                     "
     gene            complement(3114..4367)
                     /locus_tag="VFQ35_11295"
     CDS             complement(3114..4367)
                     /locus_tag="VFQ35_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9931268.1"
                     /translation="MSTEIASDPMNPSLSAPEKTTTAASVAPRVGTARRRWIALGVAA
                     VALASGGALLASRHGAGSPAKAAEASDTPRVEGDRIAYSPAFAERAGIRLVPVDKSQL
                     VPVVTAVGTVDFNPEHVAAVGTRLRGLVSRVVKFEGDTVEKGDVMAVIDSAELGEAQA
                     AVSMLEAEKHAADVNLRRENELSQKNLTTQREAEVASVEAQKYQLLLGAARQKVSALA
                     GGVGSRPSGRLGAHELRAPIKGTIVARGIAPGQSVESNLVAFRVANINHLWVELDVFE
                     KNLAKIRLGDRVELSPLAAPQEVFEGRVARIGALIDADTRSAPVRVEVDNHDRRLRAG
                     QAVNARIHAAGGEHASTQVPSSAITYVDGKPTLFVASGNNSVRVVKVELGITNGHETE
                     ITSGVNPGDQVVSEGVFALKSELFR"
     gene            complement(4364..5653)
                     /locus_tag="VFQ35_11300"
     CDS             complement(4364..5653)
                     /locus_tag="VFQ35_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9931269.1"
                     /translation="MPRSRDIFFRFISVLFVGAAPLTALAEPSTVPRDAAAALPRQPE
                     SPTAAPSLRDLVTRAREHAPEVALGRAQLEASRSSYASARMAPVGNPYLEIRADHGNR
                     GVTRDVYIDGSLWLPLEVSGQRKSRGREAADFVAWNAALVEQARAAAAARTVRAYGLL
                     VVAEKRLRVLQELSEATEAEARYYSERLAMGDATERDAALSAVDAARHKMLLSETRSE
                     LLRANGELVELTGVRLSDDVPRDPLPSIFSRTPAPAPHVEKAPSLRALQAQAAYYASA
                     RERLRKEGQSPVSVGVLAGRGDFGETRLGAGLGYAFPLFRTNQPERARADAERVRALQ
                     ELSLRREVFSRRVEVLRQELVELRKAFDVLTEAALPAAERAVAAARETYRAGKGDLLS
                     VLVSRRDFSALALRQLEILDRSWQLFSELVEVTGELP"
     gene            5958..>6518
                     /locus_tag="VFQ35_11305"
     CDS             5958..>6518
                     /locus_tag="VFQ35_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931270.1"
                     /translation="MQGISFAAATFFSTAFAGAQPLFDNEAPTDTTDPEVRPSDAKKK
                     SRGGKKKKKNPVVSPAGRGELKGRLVARTEMRAHRGTIVNTSGNVENGLIKSLDFSVA
                     TARLSFHYESPAPWLTGVVELELAGKPDLKDGYLQARFSRFQLRAGQFKSPVAPQESV
                     APFVLPTVHRGFTSHLLTDWLDVGGRR"
BASE COUNT         1159 a         2217 c         2080 g         1062 t
ORIGIN      
        1 ccgctcgagc ctcaggctcc tccgcctcgg ggcgacgata gccagcgagc atgatgcgga
       61 gcacgccggg cagcacgaac accgtcagca gcgtgctgat cgccatgccc aacacgaccg
      121 cagtcgcgag cgggcgctga acctcggcgc cagcgctcgt cgccgtcgcc atgggcaaga
      181 acccgagcgc cgcgacagcc gcggtcgtga gcaccgcccg cagcgtcgcg ccgcagcccc
      241 ccgtcacggc atcgtccagg agttgtcccg cgtccagccg cttgcgcact tcactagcca
      301 tcaccacgcc gttcagtacc gcgatgccgc ccagcgcgat gaagcccact gccgcgggca
      361 agctgaatgg catactgcgc agcacgagcc cgagcatccc accggcgagc gagagcggca
      421 cgagcacgaa cacggcgatc gtgagccgta gattttggaa catccacagc aacatgccga
      481 agatcagcat gatgacgacc ggcaccacga tcgcgaggcg cgcttgtgcg cgctcgaagt
      541 tctcgaattg cccgccccac tcgacgcggt acccggtggg cagaggcacg ctgtccttca
      601 cggccgcctg cgcctcggag acccaggaga ccagatcgcg cccgcgcaag ttcacgtcga
      661 ctcgcaccgc acgcgtacgg ttctcgcgac gaatcgcggt tggtccctcc ccttcggtga
      721 tcgagagcac ctcgcgcaac gggacgtttt cggagttacg agtcgtgacg aacaggtcgc
      781 cgataccgtc ggcggtggga cgcgcgggtg gctcgagaac gcgcagctcg aagcgacgct
      841 cgtcttcgta caccggtccg accgggacgc cttcgcgcgc cgcggccaac accgcaaacg
      901 catcttcgac ccgtacgcca tagcgagcga gccggccgcg atcggcgacg gcgctgatgg
      961 tgggcgcgcc gagcacacgc tcgacgcgca cgtcgccggt cccctgaatc ttacggacgc
     1021 ggttaccgac cgattgcgcg agcttcatca gggtatcgag ctggtcaccg aagatctgaa
     1081 tctgcacgtc cgcgcgcgag ccgctgatga gctcattcgt tcggtcttcg atcggctgcg
     1141 acaccgaaac gaacgtgccg ggcaccttgt tctcgatttg agttttgaaa agctcggaga
     1201 gctcgtcgaa gtcatgcgcg ctcgtccatt cgcccagggg gcggaggcgc acgaacaggt
     1261 cggtgttgtc gttgcccacc gggtccaccg cgacttcggc tcgccccgtc attcccagag
     1321 tagtgaccac ctcgggaaag gagtgcagga ccttctcggc atcgaggtcg agcttgcgtg
     1381 cggcctcgag cgagatgctc ggcgcgcgtc ggatggtcac caccgcgtcg ccctcgaaga
     1441 tgcgcggcac gaactcggcg ccctggctcg tgaagagcca cgccgacacg ccgaacacgg
     1501 cgacgccgcc ggctacccac acgacgcgaa agcgaatgat ggaggcgagc atgcgctgat
     1561 acagcgcggt cagtcgctcg agccagtgcg gcccgtgccc ggccgcgggc ggaaccaaca
     1621 cgaccagcac cgcagggaag aagagcacgg cataaataag cgcgccgaag agggcgctcg
     1681 ccatcgtcac ggccatgggc cggaacatct tcccctcgac accttcgagc gcgagcaagg
     1741 ggatgtagac gagcatgatg atggccaccg cgaacgcgac cgggcgcgcg accgcgccgg
     1801 caatttctcc gtaagccccc gctcgagccg ctccgacgag cttcttgccg gcggtggcgg
     1861 cgatgaccgc ttccaagatg acgattgggc cgtctaccaa gaagccgaaa tcgatcgcgc
     1921 cgagtgacat caagtcaccg gtgacgtgga acaagtgcat gccgatcagc gccacgctca
     1981 tcgcgcaagg gataccgagc acgaccgcga gcgcgccgcg ccaggtaccc aagaacagga
     2041 tgagcacgat ggtcacgatc gcgatgcctt cggccaggtt cttcatcacg gtcgagagcg
     2101 tgcgcccgac gaagtcgctg cgatcgtaga tgacctcgat ttttacgccg ggaggcagct
     2161 tcgtttggat ctccgcggcg cgcttcgcca ccgcggtcac gacgttccgg ctattctcgc
     2221 cgagcagcat catcacgacg ccggttacgg cttcaccctt gccgttgtga gtgatcacgc
     2281 cctggcggag cgccgccccc accttcacgt cggcgatgtg atggatcagc accggcatgc
     2341 cctccggtgt cgtgcgcacc atcacgttgc cgatttcgtc agggccgctc agcatgccct
     2401 ggccgcgcac ggtgaacgac tcctcgcgcc gatcgatgta accgccgccc gcgttcatgt
     2461 tggcggcccg aagcgcgtcg acgacgtcgc cgagcgagag cccttgcgcc ttcagcttcg
     2521 cgcgatcggc gacgacctga aactgcttca ggtcgcctcc catcgtgttc acctcgacga
     2581 cgccgggaac gttccgcagc ttcggcacga tttcccagtc gagcagcgtg cgcagctgca
     2641 tcgccgagtg ctggtcgcta cgaaccacga actggaagat ctccccgagg ccggacgaaa
     2701 ccggcgcgag ctccggcgcg cccgccgaag ctgggagctc gacgccgcgc aaccgctcga
     2761 gcaccagctg gcgcgcgaac caaatgtcca tcgtctcgcg aaatatgacg gtcaccgccg
     2821 acagacccga gcgcgatacg ctgcgttggt cgatcgcgcc ggggacgccg ttcagcgcgt
     2881 tttcgatcgg catcgtgacc gttcgctcga tctcgaccgg cgagagcccc gaggccgaag
     2941 tgagcaccga cacctggacg gtggacacgt ccggcagggc atcgatcggc agcgtgcgcg
     3001 cggcccaaac cccgacgccg agaagcagga agagcgtcgc gaaaaccgcg ccccgcgccc
     3061 gaatggacgc caataccaag aatttcagca tgattcagcg gctcgggtaa aggtcagcgg
     3121 aagagctcgc tcttgagcgc aaagacgcct tcgctcacga cttggtcacc cgggttcact
     3181 ccgctcgtga tctccgtctc gtgaccgttg gtgattccga gctcgacctt caccacgcgc
     3241 accgagttgt tgccgctcgc gacgaacagc gtgggctttc cgtcgacgta ggtgatcgcg
     3301 ctgctcggca cctgggtgct cgcgtgttca ccgccagccg cgtggatgcg cgcgttcacg
     3361 gcctggcctg cgcgcaagcg ccgatcgtgg ttgtcgacct cgacgcgcac cggcgcgctc
     3421 cgcgtgtcgg cgtcgatgag ggcgccgatg cgcgcgacgc gtccctcgaa cacttcttga
     3481 ggagccgcga gcggactcag ctcgacgcgg tccccgagcc gaatcttggc gaggttcttc
     3541 tcgaacacgt cgagctcgac ccacaggtga ttgatgttgg cgactcgaaa cgcgacgagg
     3601 ttgctttcca ccgactgccc cggcgcgatg ccgcgcgcga cgatggtgcc cttgatcggc
     3661 gcgcgcagct cgtgggcacc gaggcgaccg gaggggcgac tgccgactcc gccggcgagc
     3721 gcggagacct tttgccgcgc agcgcccagc aagagttggt acttctgcgc ctcgacggac
     3781 gcgacctccg cctcgcgttg cgtggtgagg ttcttttgcg aaagctcgtt ctcgcggcgc
     3841 aggttcacgt cggcggcgtg cttctcggct tcgagcatgc tcaccgcggc ttgggcctca
     3901 ccgagctcgg cgctatcgat gacggccatc acgtcaccct tctcgaccgt atcgccctcg
     3961 aacttcacga cccgactcac gaggccgcgt aagcgcgtac cgaccgcggc gacgtgctct
     4021 ggattgaagt ccaccgtgcc caccgcggtc acgaccggca cgagctggga tttatcgacc
     4081 ggcacgagcc ggatgcccgc gcgctccgcg aaggcgggcg agtacgcgat gcgatcgccc
     4141 tcgacgcgcg gcgtgtcact cgcttcggcg gctttggcag gactgccggc gccgtggcgc
     4201 gaggccaaca aggcgccccc actcgcgagc gctaccgccg ccacgccgag cgcaatccag
     4261 cgccggcgag ccgtgccaac gcgcggcgcc acgctggcgg cagttgtggt cttttcaggg
     4321 gcagacagcg atgggttcat tggatccgat gcaatttcgg tactcatggg agctctccgg
     4381 tcacttcgac gagctcgctg aagagctgcc aacttctgtc caaaatctcg agctgtcgca
     4441 gcgcgagggc cgagaaatct ctgcggctca ccaagacgct cagcaagtct cccttgccgg
     4501 cgcggtacgt ctcacgagcg gccgccacgg cgcgctccgc cgcgggcagc gccgcctcgg
     4561 tcaacacgtc gaacgctttt cgtagctcga cgagctcctg acgtaacact tccacgcggc
     4621 ggctgaacac ctcgcgacgc agcgagagct cttgcagcgc gcgcacccgc tcggcgtcgg
     4681 ctctcgcgcg ctctggctgg ttcgtacgaa acagcggaaa tgcgtagccg agccccgctc
     4741 cgagccgcgt ctcaccaaaa tcgccacgcc ccgccaaaac accgacgctg acggggctct
     4801 gcccctcttt gcggagacgt tcgcgagccg acgcgtagta ggccgcctgc gcctgcagcg
     4861 cccgcaacga cggcgccttt tccacgtggg gcgccggcgc cggagtgcga gagaaaatgc
     4921 tcggcagtgg gtcgcgagga acgtcgtcac tcaggcgaac acccgtgagc tcgacgagct
     4981 ccccattcgc gcgcaagagc tcgctccgcg tttcgctgag cagcatcttg tggcgcgccg
     5041 cgtccactgc cgagagcgcc gcgtcacgtt ccgttgcgtc gcccatggcg agccgctccg
     5101 agtagtagcg ggcctccgcc tcggtcgctt ccgagagctc ttgcagaaca cgcaggcgct
     5161 tctccgctac gacgagcaag ccgtacgcac gcacggtgcg agccgcggca gccgcgcgcg
     5221 cttgctcgac gagcgctgcg ttccaggcga cgaagtcagc cgcctcgcgc cctcggctct
     5281 tgcgctgacc cgagacctcg agcggcagcc acagcgagcc gtcgatgtag acgtcgcgcg
     5341 tcacgccgcg attgccatga tcggcgcgga tttcgaggta ggggttaccg acgggcgcca
     5401 ttcgcgcgct cgcgtagctc gaccggcttg cctcgagctg tgcccttccg agcgcgactt
     5461 ccggcgcgtg ctcacgcgct cgggtgacga ggtcgcgtaa cgacggagcg gccgtgggtg
     5521 actccggttg gcgaggaagc gcggccgcgg cgtcgcgggg cacggtcgat ggctccgcga
     5581 gggctgtgag aggtgctgct cccacgaaga gcaccgagat gaaccggaag aagatgtcgc
     5641 gcgaacgagg catgtgggag taaaaggaaa tttccgagct ttgagcgttt ggcccctcga
     5701 cccaggaacg agcatcgtcg tgccgggtat cgaagacatt gcagaataat ttcttgagcg
     5761 gcttgcgcaa tgaaacaaag ttgtcctttg aatgttcccg caaatctctg caatttaaat
     5821 taccgaaacg ttacaagacg atgtggccga gactgaattg tccttccggc ctcgtatcca
     5881 tggaaaaata ttacattaac ggcccgtcgt gtgggccgtc tcggttgcgc tcgggaggta
     5941 agtgcaagtc gctttggatg cagggaattt cattcgctgc agctactttt ttttcgactg
     6001 ccttcgccgg cgcgcagcct ctcttcgaca acgaggcgcc cactgacacg accgatccgg
     6061 aagttcgccc gtcggatgcg aagaagaaat cgaggggtgg gaagaaaaag aagaagaacc
     6121 cggtggtgag cccggccggg cgaggcgagc tgaagggccg cctcgtcgcg cgaaccgaaa
     6181 tgcgggcgca ccgcggcacc atcgtcaaca cgagcggcaa cgtcgagaac gggctcatca
     6241 agtcgctcga cttctccgtc gcgacggcgc gactctcgtt ccattacgaa agtcctgcgc
     6301 cttggctcac cggcgtcgtg gagctcgagc tcgctggtaa gcccgacttg aaggacggct
     6361 acctgcaagc gcgcttctcg aggtttcagc tgcgcgcagg gcagttcaaa tcgccggtcg
     6421 cgccgcaaga atcggttgcg cccttcgtgc tccccaccgt gcaccgcggg ttcacgagcc
     6481 atttgctgac tgattggctc gacgtcggcg gacgcagg
//
LOCUS       DASTKS010000547         5492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_532748, whole genome shotgun sequence.
ACCESSION   DASTKS010000547 DASTKS010000000
VERSION     DASTKS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532748"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFQ35_11310"
     CDS             complement(<1..437)
                     /locus_tag="VFQ35_11310"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9931271.1"
                     /translation="MFVITGATGHTGSVAAETLLAQGHKVRVVVRDAAKAKGLEARGA
                     EVFVADLEDQEALARAVRGADGVYLLSPPDMGAKDFVAERKRLTERQVNTLAAEKVPH
                     VVLLSSVGAQHTSGTGPILTTHNVEQQLRAAGLNATFVRAAYFV"
     gene            515..1432
                     /locus_tag="VFQ35_11315"
     CDS             515..1432
                     /locus_tag="VFQ35_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9931272.1"
                     /translation="MHMVHDSALSGIDLNLLVVLRALLTERHVTRAAARVGLSQSATS
                     HALARLRELYGDPLLVRSGRALSLTPRAARLLPAVERGLGELENTMRDEPEFQPSTAR
                     RTFTLGMADYLQALIIGPLLQKVAKRAPGIDLSVVVFPNLRELVESGSVDLALTVSSA
                     DLRGLSHEPLFEEGFMCMVRRGHPRIRKTLTLASYLAERHVAIAPGGTPGTLVDTVLA
                     QHGLERRIALRVTNFLIAPVVVSQTDFINTMPTRLARQLASTYALRLVPSPIELPVFE
                     YCMTWHPRLDRDPAQHWLRDIVATATKAG"
     gene            complement(1510..3036)
                     /locus_tag="VFQ35_11320"
     CDS             complement(1510..3036)
                     /locus_tag="VFQ35_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9931273.1"
                     /translation="MRGWSIGYVAALISSCAALPLSSCAGSGGSGAEAGTGGTGASSG
                     AGSASGGVAGAAAFGGAADGGSPSDGGRSATSGGRATNGGASARGGSSSGGGSARGGG
                     TSGGGVTGGGTSATGGAPSGGAGGDGGVLVAQWNDAPGQCPSGAVRADIRTVAEMQSA
                     SRGESNQNATCFFVHDGTYAQSGSTLPLYIKRGGTPGAPVVWVGESRDGVIVKGRATF
                     EVGADHVLLSNMTFDISALTQSGAYNTITVLANDIVMSHLTLTGDCAHGSRGGHIEVP
                     GPTQTHVIIDSCIIEKFGHCSADGSLDHGVYLSGGNDIQVINNIVRENSSRGIQLYSH
                     YEDTSQTLTNVLIERNRIESNGHGDYQDGIVIDGNKDAGVDGTIDTVVIRNNVFWHNR
                     YSGVRFVGNAVKGVQISNNTFVENGVASTSKSRSELNLDGGTPSFSATRNIFVPARAV
                     INSCLDSGGISDNVVFPASGSPACVTSSVEADPDFVDAANGDFHPRNPTVARYGSYAP
                     "
     gene            complement(3360..3770)
                     /locus_tag="VFQ35_11325"
     CDS             complement(3360..3770)
                     /locus_tag="VFQ35_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931274.1"
                     /translation="MGWRFRDRLSVIVHTPKNPSNLEWQGLLRDRALHGRDSNERTLI
                     VSYGGGPDADQRKLLSREMDGRAAPTVIMTKSAMVRAIASALLFFNRQMKVVGIDERE
                     LAYQFLGFSPDESRIADRLRAELELELGIAKLCA"
     gene            complement(4027..>5492)
                     /locus_tag="VFQ35_11330"
     CDS             complement(4027..>5492)
                     /locus_tag="VFQ35_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931275.1"
                     /translation="VQTLLTDELPGAAVLATLQQLRAKSLVLAGDDDFALLAPVRDYA
                     LGELSRRGLEESLTARHRDYQATRVRELLRLRETEGARRTLVRLEREADDVLAAVEHA
                     LGAPNPQIGFVLGALVALEPLISARGPLPAFTEILSRAISAADAESVRGFVELLSRVL
                     LLRGRLLTTSGRFQQGREDLLRARSLGEQVESDELLGAILLEIGVNHHFQRELAAARA
                     CYERALELCRGARQGAVRGRCYGNLGAVLHDEGRLTEAAAHYWKAIRALEEGGEAREL
                     GNFLSNLAVLEQELGARADARRHYQQALAYLGELAETRLHAIALGNLGVLEAEDGAWE
                     AALKCHEQALSLLQTQNDAFSEALCRARLGAALAVLRREDEANEQLSHARRMIVEADA
                     PRIAAVRLQRAFLDFARARRALEGGERSEARERLRLAEDVSAEIASELHEGVKLTTLS
                     DDVRAALRLLAPLVRGVRHRLERDAEPPSSPTLVAER"
BASE COUNT          906 a         1861 c         1773 g          952 t
ORIGIN      
        1 acgaagtagg ccgcacgcac gaaggtcgcg ttgagccctg ccgcccgcag ttgctgctcg
       61 acgttgtggg ttgtcaatat cgggcccgta ccagaggtgt gctgggcgcc aaccgacgaa
      121 agcagcacga cgtgggggac cttctccgcg gcgagcgtat tcacctgtcg ctcggtcaag
      181 cgcttgcgct ccgcgacgaa gtccttggcg cccatgtccg gcggcgagag cagatacact
      241 ccgtcggcgc cgcgcacggc gcgcgcgagc gcctcctgat cttcgagatc tgcgacgaag
      301 acctcggccc ctcgcgcctc gagccctttg gccttggccg cgtcgcgaac tacgacgcgc
      361 accttgtgtc cctgagcgag cagcgtctcc gccgcgaccg aacccgtatg acccgtagca
      421 ccagtaatga cgaacatctg agttcctcct gctgacgacg agatctttgg gctcgaatga
      481 gtcatgcgtc caatgcaatc ttggcactat actcatgcat atggtgcatg acagcgctct
      541 ctcgggaatc gacctgaacc tgctcgtggt cttgcgggcg ctcttgaccg agcggcacgt
      601 gacgcgcgcc gccgcccgcg tcgggctcag ccaatcagcg acgagccacg cgctcgctcg
      661 cttgcgcgag ctgtacgggg accctctgtt ggtccgaagc ggtcgcgccc tgagcctcac
      721 tcctcgcgcc gcacggctgt tgcccgcggt cgagcgcgga ctcggcgagc tcgaaaatac
      781 catgcgggac gagccggagt ttcagccgag cacggcgcgc cgcaccttta cgctcggcat
      841 ggcggactac ttgcaagcgc tgatcatcgg accgctcttg cagaaggtcg cgaagcgcgc
      901 gccaggcatc gatctctcgg tggtcgtctt cccgaacctg cgcgagctcg tcgagtctgg
      961 ctccgtggac ctggccctca ccgtttcgag cgctgatctg cgcggcttga gtcacgagcc
     1021 cctattcgaa gagggcttca tgtgtatggt ccgccgcggt caccctcgaa tcagaaaaac
     1081 gctgactctc gcgagctatc tcgcggagcg gcatgtcgcg atcgcgccgg gcggcacgcc
     1141 gggaacgctg gtagacaccg tgctcgcgca gcacggactg gagcgtcgta tcgcgctgcg
     1201 cgtcacgaat tttttgatcg cgccggtggt cgtttcgcaa acggacttca tcaacaccat
     1261 gccgacacgc ctcgctcgtc agctcgcgag tacctacgcg ctgcggctcg tcccctcgcc
     1321 catcgagctg cccgtattcg agtactgcat gacatggcac ccgcgcctcg atcgcgatcc
     1381 ggcgcagcat tggctgcgag acatcgtcgc gaccgcgacc aaggccggct gacgccgtcg
     1441 aaaacggcag ccgactggtc gcggcgtttc ccgcccggga ctgcctgcag cgaggcgaat
     1501 gcgtgtccgt cagggcgcgt agcttccgta tcgggccacg gtcggattac gcggatgaaa
     1561 gtcgccgttg gcggcgtcga cgaagtccgg atctgcttcg accgacgaag tgacgcaagc
     1621 ggggctgcca gatgcgggga agacgacgtt gtccgagata ccgcccgagt ccaagcacga
     1681 gttgatgacg gcgcgggcag gcacgaaaat gttccgtgtc gcggagaagc tcggggtacc
     1741 accgtccagg ttcagctccg aacggctctt gctcgtcgat gcaacgccgt tttcgacgaa
     1801 ggtgttgttg cttatctgca cgcctttgac ggcgttcccg acgaacctca cccccgagta
     1861 gcgattgtgc caaaagacgt tgtttcggat caccaccgtg tcgatcgttc cgtcgacccc
     1921 tgcgtccttg ttgccgtcga tgacgatgcc gtcttggtag tcgccgtggc cgttcgactc
     1981 gatgcgattg cgctcgatga gcacgttggt gagggtctgc gacgtgtctt cgtagtggga
     2041 gtaaagctga atgccgcgcg aggagttctc gcgaacgatg ttgttgatga cctggatgtc
     2101 gttaccgccg gagaggtaca ccccatgatc gagcgagccg tccgcgctgc aatgcccgaa
     2161 cttctcgatg atgcacgagt cgattatgac atgggtttga gttgggcccg ggacctcgat
     2221 gtgtccgccg cgcgagccgt gcgcgcaatc gcccgtcaaa gtgaggtgtg acatgacgat
     2281 gtcgttcgcg agcacggtga tcgtgttgta ggctcccgac tgcgtgagcg ccgagatgtc
     2341 gaacgtcatg ttcgaaagga gcacgtgatc ggcgccgacc tcgaacgtgg ctcggccctt
     2401 cacgatgacg ccgtcgcgcg actcgccgac ccaaactacg ggagcccccg gcgtgccgcc
     2461 gcgcttgatg tatagcggga gagtgctgcc gctctgcgca taggtgccgt cgtgcacgaa
     2521 gaagcacgtc gcgttctggt tgctctctcc acgagacgcg ctctgcatct cggcaacggt
     2581 tcgaatgtct gcgcgaaccg cgcccgaagg acactgcccg ggagcgtcgt tccattgcgc
     2641 cacgagcacg ccgccgtcgc cacctgcacc gccgcttggc gcgccaccgg tcgcgctcgt
     2701 tcctccaccg gtaacgccgc cacccgaagt gccgccaccc cgagcgctgc caccgccgga
     2761 cgaactgccg ccgcgcgcgg acgctccgcc gttcgtagcg cggccgccgc tcgtagccga
     2821 gcgccccccg tcgcttgggg aaccgccgtc cgctgcaccg ccaaacgcgg ccgcaccggc
     2881 gacgccaccg ctcgcgctgc cggcgccgct gctagcaccg gtcccccccg tgcccgcttc
     2941 ggcaccggag ccgccgctgc ctgcacagct cgaaagcggc aatgcggcac agctcgatat
     3001 caaggcggcg acgtaaccaa tggaccaccc acgcatgact catggtccgc tccctcgggc
     3061 agtcgagtca atcagcgagc gcaatccacc gccgagttcg ctctcgtctt ggggtgcgac
     3121 acggtgacgc accggtcgtc cgaagctctc ttttcggtgg gggcgcgggc gcggtgggag
     3181 cgcgcccact gtcgcatggt gtctgcgctg gcgacacccg acgcctcgtt gcgcgctctt
     3241 tacatcgagg cgcccactct ccgagtgcgc tgctttgggc tcacgcgatc gcgcgcgatc
     3301 aaggtccgac tctcactctc cgacgcgcgc cgactcgagt gggtcggcct cgtcactcgt
     3361 caagcgcaga gcttcgcgat gccgagctcg agctcgagct ccgcacgcag tcgatccgca
     3421 attctggatt cgtccgggct gaagccgagg aactgatagg cgagctcacg ctcgtcgatg
     3481 ccaacgacct tcatctgacg attgaagaag aggagcgccg atgcaattgc gcgcaccatt
     3541 gcgctcttcg tcatgatcac cgtcggcgcg gcccgaccgt ccatttctcg cgagaggagc
     3601 ttgcgttggt ctgcgtcggg gccgccgcca tacgagacga tcagcgtgcg ctcattcgaa
     3661 tctcgcccgt gcaacgcgcg atcgcgtaac agcccttgcc actccaggtt actggggttc
     3721 ttgggggtgt gcacgatgac cgacaatcgg tcgcgaaacc gccagcccat cgtcgactcc
     3781 atggtcttta cggtaggcga cgccccacgc gcgtcaaaca ccaagcgaca aatatcacgc
     3841 tggcgtcgag cggcatcgta gggagaacac cgacgacacc gacttacact ccaaaatccg
     3901 gcgtgaagtc gcgacctcgt tcgaagcgcg cagcgaggtg gcaacgctgc cggcggcgtc
     3961 cgacagcgcg agctcgcgca cgcggagtgc ggcgtcgtcc ccgatcgtag gttgctgggt
     4021 cgcacgtcag cgttcagcta cgagcgtcgg gctagacggc ggctcggcat cgcgctcgag
     4081 gcggtgccgc acgccgcgca cgagcggcgc gagcagccgc agcgcagcgc gaacgtcatc
     4141 cgacaacgtc gtgagcttta cgccttcgtg cagctcgctc gcgatctccg cggagacgtc
     4201 ctcggcgagc cgcagccgct ctcgagcctc actacgctcg ccgccctcga gggcacgacg
     4261 cgctcgcgcg aaatcgagga acgcgcgctg gagacggacg gctgcgatgc gtggcgcgtc
     4321 ggcttcgacg atcatgcgac gggcatgcga cagctgctca ttggcttcgt cttcgcgccg
     4381 cagcaccgcg agcgccgctc ccaggcgtgc acggcagagc gcctcggaga aagcgtcgtt
     4441 ctgcgtctgc aagagtgaga gagcctgctc gtggcacttc agcgctgcct cccaggcgcc
     4501 gtcctctgcc tccaagacgc ccaggtttcc gagcgcgatg gcgtgtaggc gcgtctcggc
     4561 gagttcgccc aaatacgcga gcgcttgctg gtaatggcgc cgggcgtcgg cgcgcgctcc
     4621 gagctcctgc tcgagtacgg cgaggttcga aaggaagttg ccgagttcgc gcgcctctcc
     4681 accttcctcg agcgcgcgga tcgccttcca gtaatgggcg gcggcttcgg tgagacgccc
     4741 ttcatcgtgc aagacggcac ccaggttgcc gtagcaacga ccgcgcacgg cgccctgccg
     4801 agcaccgcgg cagagctcga gcgcgcgttc gtagcaggct cgcgccgcgg cgagctctcg
     4861 ctgaaaatgg tggttcaccc cgatttcgag gaggatcgcg ccgagcagct cgtccgactc
     4921 gacttgctcg ccgaggctgc gcgcgcggag caggtcctcg cggccttgct gaaaccgccc
     4981 cgaggtcgtg agcaagcgac cgcgcagcaa gagcactctc gacagaagtt cgacgaagcc
     5041 tcgaacgctc tccgcgtcgg cggcgctgat cgcgcgactc aagatctcgg tgaacgccgg
     5101 cagcggtcct cgcgcgctga tcagcggttc cagagcgacc agcgcgccga gcacgaagcc
     5161 aatctgcgga ttgggggcgc cgagagcgtg ctcgacggcg gcgagcacgt cgtctgcctc
     5221 tcgctctagg cgcacgagcg ttcgtcgcgc accctcggtt tcgcgcaagc ggagcagctc
     5281 gcgcacgcgc gtggcttgat agtcgcggtg acgcgccgtg agcgactctt cgaggccgcg
     5341 ccgggagagc tctccgagcg cgtagtcgcg aacgggagcg agcagcgcga agtcgtcgtc
     5401 tcccgcgaga acgagtgact tggcgcgcaa ctgctgcaag gtggccaaaa cggccgcgcc
     5461 cggcagctcg tcggtgagca gcgtctgaac gg
//
LOCUS       DASTKS010000548         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_86288,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000548 DASTKS010000000
VERSION     DASTKS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86288"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="cas4"
                     /locus_tag="VFQ35_11335"
     CDS             complement(<1..674)
                     /gene="cas4"
                     /locus_tag="VFQ35_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HET9931276.1"
                     /translation="MVNEYEYCPRLAYLEWVQGEWADSADTVEGRHTHRRVDRRSGGL
                     PEPDPEAESTGERIHARSVALSSTRLGLTAKIDLVEVDGNTVTPVDYKRSKRPHVPKG
                     AYDPERVQVCAQGLLLEEHGYNCTEGVLYFAGSKERVRVVFDDELREQTLAAVNGLRF
                     VAAGGRMPPPLEDSPKCPRCSLVGICLPDEVNFLRRTEVGARPLAVGRDDALPLYVQA
                     RQAKVA"
     gene            1163..2167
                     /locus_tag="VFQ35_11340"
     CDS             1163..2167
                     /locus_tag="VFQ35_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HET9931277.1"
                     /translation="MYALHAMLCDRRTPVAREALMRELECSEQTIYRLIRAMKDYLGA
                     PIEWNEELGGYYYDERSAQGLRYQLPGLWFNAKELQALVVFDKLFETLEPGLLGEHLA
                     PLTRRISDLLEHKRLGLGEVARRIRVLGMASRPVGPCFHTLASATLQRRRVHIVYHGR
                     ERNRVTERVVSPQRLVHYRDSWLLDAYCHTREGLRTFSIDRVREARELAEPATTIPDA
                     ELDEYFASAYGIFAGRANKTAVLRFSAERARWVADERWHPQQSGQFETDGRYTLEIPY
                     RDDRELVMDILRHGGEVEVIRPAALREAVRKELSKALGLYGRSTVPMRSERPSDGEDA
                     "
     gene            2257..>3643
                     /gene="rmuC"
                     /locus_tag="VFQ35_11345"
     CDS             2257..>3643
                     /gene="rmuC"
                     /locus_tag="VFQ35_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET9931278.1"
                     /translation="MTDHLIAVTAFLAGTAFAWWVRSTIVAGALRASRARIDDLTRQL
                     GEERRELEALSREHDRLAERLRSEIERRARAESDLDNERKGSTEKLAVIQQAETALSD
                     AFKALSAEALKSNNRAFLDLARATLEKHQESARNELEARQKAVDELVRPMRESLEKVD
                     GKLGEIEKVRIGAYAALDQQLKSLVETHLPALRGETANLVKALRQPAVRGRWGEIQLK
                     RVVEMAGMLGHCDFKEQESRATEDGRIRPDLVVRLPGGKQIVVDAKAPTEAYLKALEA
                     SDETEQRALLADHAAQVRSHMLSLGRKSYWEQFEATPEFVVMFLPGEVFFSAALQQDP
                     ALIEFGVNERVIPATPTTLIALLRAVAYGWRQEALAQNAQEVAELGRQLYERVASLAK
                     HWSDVGDRLDKAVESYNRSVGTLESRVLVSARKLSELRTAPEGVEIRVIEPVERTTRR
                     LTAVELLASSSN"
BASE COUNT          692 a         1091 c         1238 g          622 t
ORIGIN      
        1 ttcgcgacct ttgcctgtct cgcttggaca tacagcggca gcgcatcatc gcgaccgacg
       61 gcgagcggtc gtgcgccgac ttctgtgcgc ctgaggaagt tgacttcgtc cggcaggcag
      121 atcccgacca gagagcagcg agggcacttg gggctgtcct cgaggggcgg cggcatgcgt
      181 ccgccggccg ccacgaaacg caatccgttc acggccgcca gcgtttgctc gcgcagctcg
      241 tcatcgaaca cgactcgcac tcgttccttc gagcccgcga agtacagcac accttcagtg
      301 cagttgtagc cgtgctcttc gagaagcaac ccttgagcgc agacttgaac gcgctccgga
      361 tcgtaggcgc ctttcggcac gtgcggtcgc ttgctgcgct tgtagtcgac cggagtgacc
      421 gtattgccgt cgacctcaac gaggtcgatt ttggctgtca agccgagccg ggtagaggac
      481 agtgccacgg atcgagcgtg aattctctcg ccggtcgatt cagcttcagg atccggttcg
      541 ggcagaccgc cgctacgtcg atcgacacgg cggtgtgtgt gccgcccctc gacggtgtcg
      601 gcggaatcgg cccattcccc ctgcacccat tcaagatagg cgaggcgggg gcaatactcg
      661 tactcgttga ccatgcgtac cggcaagagc ggtacatcgc cggaatgctc ggggaaaggc
      721 agctgcagat ccggttgtcg aggttcatcc atacgcctta cctcatccga tgggctgcgc
      781 agtcttgcgc gaatcggccg acgaatgata aagagccaag cttaagaagc tgtcggtgct
      841 cggaacgtga ttaccggcac atcctcggcg gttcaaagcc gcggaagagc gaatggatcg
      901 gtgcaaatgc gaagtcgctc atagtgcagc acggcttgca aagcgctccg gctttgagaa
      961 tcggccggcg ggtcatatga ttcgcatggc aaagcaaagc gaatagcttc tgccgggaag
     1021 gtgtctgcct gggcggcccg agcatgcatg gggcttcaat ccgcgccgcc cgcgggtgcc
     1081 gagagagcag agttcgcgac gagaagaata accagggtcg cgacctgaat cgggggcgtc
     1141 aaagctggac aagttcgatc ggatctacgc gcttcacgcg atgctctgcg atcggagaac
     1201 gccggtcgct cgcgaggcgt tgatgcggga gctggagtgc tcggagcaga ccatctaccg
     1261 cctgattcgc gccatgaagg actacctcgg cgctccgatc gagtggaacg aggaactcgg
     1321 cggttactac tacgacgaac gcagcgccca gggcttgcgg tatcaattgc ctggtctctg
     1381 gttcaacgcg aaggagctcc aagcgctcgt cgtcttcgac aagctcttcg agaccctcga
     1441 gccgggcctg ctcggcgagc atctcgcacc gctcacgcgg cgcatcagcg atctcctcga
     1501 gcacaagcgg ctgggcctcg gggaagtcgc gcggcgcatt cgtgttctcg gcatggcatc
     1561 ccggccggtc gggccgtgtt tccacacact ggcgtcggcg acgctccagc ggcgtcgggt
     1621 tcacatcgtg tatcacggcc gcgagcgcaa tcgcgtcacg gaacgcgtcg tttcgccgca
     1681 gcggctcgtt cactatcgcg atagctggct gctcgacgcg tactgccaca cgcgcgaggg
     1741 gttgcggact ttttcgatcg accgcgtgcg cgaggcacgc gagcttgcgg agccggcgac
     1801 gacgattccc gacgccgagc tcgatgagta tttcgcgagc gcctacggaa tcttcgcggg
     1861 gagggcgaac aagaccgcgg tcctgcggtt ttcagcggag cgcgctcgct gggtcgcgga
     1921 cgagcgctgg catccgcagc agagcgggca gttcgagacc gatgggcggt acacgctcga
     1981 gatcccgtat cgcgacgatc gggagctcgt catggacatc ttgcggcacg gcggcgaagt
     2041 ggaggtaatt cggccggctg cgctgcggga ggcggtgagg aaggaattaa gtaaagcgct
     2101 gggcctgtac gggcgttcga ccgtcccgat gcgatcggaa cgcccttcgg acggtgaaga
     2161 cgcgtgacgg ccgcctgcga accactatcg tccgatgaga gtcgcatcgg tgagactgcg
     2221 ttgggtaccg aacgaaaaca acaacgacga cgacctatga cggaccatct gatcgcagtt
     2281 acggcatttc tcgcgggcac cgccttcgcg tggtgggttc gcagcactat cgtcgcggga
     2341 gctttgaggg catcgcgcgc gaggatcgac gacctaacgc gtcagctcgg ggaggagcgt
     2401 cgcgagctcg aagcgctgag ccgagagcat gatcgtctgg ccgagcggct gcgctcggag
     2461 atcgaacgtc gcgcgagggc tgagtcggac ctcgataacg agcgcaaggg ctcgaccgaa
     2521 aaactcgcgg tcatccagca agccgagacg gcgctttcag acgccttcaa agcgctgtcc
     2581 gccgaggcgc tcaagagcaa taaccgcgcg ttcctcgatc tcgcgcgagc gacgctcgag
     2641 aagcatcagg agtcggcaag gaacgagctc gaagcacgcc aaaaggcggt cgacgagctc
     2701 gtccggccga tgcgcgaatc gctggaaaag gtcgacggga agctcggcga gatcgagaag
     2761 gtccgcatcg gcgcttacgc ggcgctcgat cagcagctca agagtctcgt cgagacgcat
     2821 ttgcccgcgc tgcggggcga gacggcgaac ctcgtgaaag ccttgcgtca accggccgtg
     2881 cgcgggcgat ggggcgaaat ccagttgaaa cgcgtcgtcg agatggccgg catgctcggg
     2941 cactgcgact tcaaagaaca agagagccgc gccacggagg acggccgcat ccgcccggac
     3001 ctcgtcgtgc ggctgccggg aggcaagcaa atcgtcgtgg acgcgaaggc gccgacggag
     3061 gcgtatctga aggcgctgga agcttcggat gagaccgagc agcgagcgct ccttgccgac
     3121 catgccgccc aagtgcgttc ccacatgctg tcgctcgggc gaaagtccta ctgggagcag
     3181 ttcgaggcga cgccggagtt cgtcgtgatg ttcctgcccg gcgaggtttt tttcagcgcg
     3241 gcgcttcagc aggatcccgc gctcatcgag ttcggcgtca acgagcgcgt cattcctgcg
     3301 acgccgacga cgctgatcgc gctgctacgt gccgttgcct acggctggcg gcaggaagcg
     3361 ctcgcacaaa acgcgcagga agtcgcagag ctcggacggc agctctacga gcgggttgcg
     3421 agcctggcga aacactggag cgacgtcggc gatcggctcg acaaggcggt cgagtcgtac
     3481 aaccggtcgg ttggaacgct cgagtcgcgc gtgctcgttt cggcgagaaa gctcagtgag
     3541 ctcaggacgg cgccggaggg ggtggagatt agggtgatcg agccggtgga gcggacgacg
     3601 cggaggctaa ccgccgtgga gctattggcc tcgtcgtcga atc
//
LOCUS       DASTKS010000549         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1201408, whole genome shotgun sequence.
ACCESSION   DASTKS010000549 DASTKS010000000
VERSION     DASTKS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201408"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFQ35_11350"
     CDS             <1..660
                     /locus_tag="VFQ35_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931279.1"
                     /translation="SGAYTRLVVEDDGIGMSEQTLERIFDPFFTTKGPGEGTGLGLSV
                     VHGVMRAHEGGIEVVSSEGSGSRFELYFPVAQAPHERPSERHGASIRGRGERILCVDD
                     EASILRATTLILERLGYEVVPHSEPSRALAELGTPAPFDAVVTDCAMPSIWGVDFVRQ
                     ILERRPNTPIVMMSGLLEPSLEKTLRELGVREFISKPATVADLGGALARLFNARASTR
                     E"
     gene            complement(632..3586)
                     /locus_tag="VFQ35_11355"
     CDS             complement(632..3586)
                     /locus_tag="VFQ35_11355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931280.1"
                     /translation="MLEAQTERPISLSPSEVRTLRGELSRAAFAAELGVTPHTIYRWE
                     LPEGAAEARRPRGKELDRLLRLRTDDPARVGVEPGARMAPAPLASSRSTEGLAEALPA
                     LERILGGDVRRGQSTMLHLLATRRTLSPNARAVAAVGLALSEIVVANDAKRALLAISS
                     ALADAEAGELDAQIAARTFAVAALVRSMQDASLFDLGHVHAYASRSESLSTQSDPEVL
                     CLTFLASLSAAMGAGDRELLERAFTRIDEVGFSGLPLMLELHLEEFLGLRPMLAGKAT
                     TATRNFEALAERAKIAGYPILAARCFAHQAQCDLDNLANPESVLELIRRARDTSNDAR
                     SAMGKHHLLLTRVEAEALLRLGRTEQSLEALSLLEIWSDETGMPPLAAIPTWMRVLHL
                     SGRVEGFQAVAQRLRDCQVPSLRGICRAYLAYLDAMEALASSADTKLTLAAFEEAETE
                     AKRWPFLMRQVLQHRVLAHVVNWDEGPARLALRRAQRFMENYPAPWVTAHLRRIEGTL
                     AAAAGNWVEARHLIESAIYTFELARDQCDAGLARYVLAVFGEAYGEAGAAEEVEKRRE
                     ALAQIGIKPPQALAVGMQRLKLNRARDPVANAQSRALDVESLVVPLQRLSVRGALPAL
                     ILRELVSIVEGLFGARRVWLEELDSRGTPHPVLGDAAVGGAQNVEFSDGAGRTFRISL
                     TGELTVTERSALTILTTATSLALESSTLRGFDERKATSGTDERLPDLPVFLAASPSMR
                     KLRGELSRLAGSNATVIITGESGVGKEVVARAVHDLSDRAAKPYVAFNCATVPRDLFE
                     GQLFGYRRGAFTGATADHPGVIRAADGGTLFLDEIGELPLDIQPKLLRFLENGEVFPL
                     GERRPIRVDVRVLAATHRNLAELVQSGRFREDLYYRLQVVPIFVPPLRDRKEDIPVLA
                     RQFLRELTRRGEPPVLAPDAVAALVAHGWPGNVRELRNVIERAMAFSPTPSVLRSEHL
                     NLTVTPVLTRER"
     gene            3847..5013
                     /locus_tag="VFQ35_11360"
     CDS             3847..5013
                     /locus_tag="VFQ35_11360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET9931281.1"
                     /translation="MRKPRRVALVVLPLLSALAFGGGAALLLASGGHETTDDAQVEGH
                     VAYVAPHVAGQVKRVLVKDNQRVQVGDVLVELDERDLNAKLLAARADWEASKAQLHAS
                     ETQLAVTRKSVNSNLVVARGGLAQAASTRETTVAAIEQARASVQAAESRRALAATELE
                     RTKNLFETGASSKANLDSDRAAFDQAQAALAEASARLASAEANRQNSSGTVESARGRV
                     LAAEAGPEQIEAAEAAVELARARVNQAKAALDQAELNVSYTRVKAEVAGVVSRRSVEA
                     GQMAAPERPMLALVPLEDTWVVANFKEDQIASMRPGQTVNVSIDAYPGKKLSGHVDSL
                     AGGTGARFALLPPDNASGNFTKVVQRVPVLIRLDAHPGIELRPGLSARATVITE"
BASE COUNT          884 a         1674 c         1762 g          855 t
ORIGIN      
        1 tccggtgctt acacgcgact cgtcgtggaa gacgacggga tcggcatgag cgaacagacg
       61 ctcgagcgca tcttcgatcc ctttttcacc accaaaggcc ccggtgaagg caccggtctc
      121 gggctttcgg tcgttcacgg agtcatgcgc gctcacgagg gcggcatcga agtcgtgagt
      181 agcgaaggtt cgggcagtcg cttcgagctc tatttcccag tcgcacaggc tcctcacgag
      241 cgaccgagcg agcggcacgg cgcgagcatt cggggacgcg gcgagcgcat tctgtgcgtc
      301 gacgacgaag cctcgatttt gcgcgcgact acgttgatcc tggagcggct gggctacgag
      361 gtggtacccc acagcgaacc ttcgcgcgcg ctcgccgagc tcggaacgcc ggctccgttc
      421 gacgcggtgg tgaccgattg cgcgatgccc tcgatctggg gagtggactt tgttcggcaa
      481 atcctcgagc gacgcccgaa cacgcctatc gtgatgatgt cggggttgct cgagccgagc
      541 ttggagaaaa cgcttcgtga gctcggcgtg cgcgagttca tttcgaagcc agccacggtc
      601 gcggatctcg gcggcgcgct cgctcgtctg ttcaacgctc gcgcgtcaac acgggagtga
      661 ccgtcaggtt caagtgctcg ctgcgcagca cgctcggggt gggcgagaag gccatcgctc
      721 gctcgatcac gttacggagt tcgcgcacgt tgccaggcca accatgagcg accagcgccg
      781 cgaccgcgtc cggcgcgagc acggggggct cacctcgtcg cgtgagctcg cgcagaaact
      841 ggcgcgcgag caccgggatg tcttccttgc ggtcgcgtag cggcggcacg aagatgggca
      901 cgacctgcag gcgatagtaa aggtcttctc tgaagcggcc gctctgcacg agctcggcga
      961 ggttcctgtg cgtcgccgcc aacacgcgca catcgacgcg gatgggtcgg cgctctccga
     1021 gcggaaagac ttcgccattc tcgagaaaac gaaggagctt cggctgaatg tcgagcggga
     1081 gctcgccgat ttcgtccaag aagagcgtgc cgccgtcggc cgcgcgaatg actccggggt
     1141 ggtccgccgt cgctcccgtg aacgcgccgc ggcgataccc gaacagctga ccttcgaaca
     1201 ggtcgcgtgg caccgtggcg cagttgaagg cgacgtacgg cttcgctgcg cgatccgaca
     1261 aatcgtgtac ggcgcgcgcg actacttcct tgccgacgcc ggactcgccc gtgatgatca
     1321 cggtggcgtt cgagccggcc aggcgcgaca gctcgcctcg cagtttgcgc atcgacggcg
     1381 atgcggcgag gaacaccggc agatcgggca gccgttcgtc ggtgccagag gtcgctttgc
     1441 gctcgtcgaa gccgcgcagg gtcgaggatt cgagcgcgag ggacgtcgcg gtggtgagga
     1501 tggtgagcgc cgagcgctcg gtcaccgtaa gttcgccggt caggctgatg cggaacgtcc
     1561 gcccggcgcc atcgctgaac tccacgtttt gcgcgccgcc gaccgccgca tctccgagca
     1621 ccgggtgcgg cgtgccgcgt gagtcgagct cttccaacca cacccgccgc gccccgaaga
     1681 gcccttccac gatcgagacg agctctcgca agatcagcgc gggcagagcg ccgcgcaccg
     1741 aaagccgctg cagcggaacc accaagctct cgacgtcgag cgcacggctc tgcgcgttgg
     1801 ccaccgggtc acgcgcgcgg ttcagcttca gtcgctgcat ccccaccgcg agcgcttgcg
     1861 gcggtttgat cccgatttgc gccagggcct cgcggcgctt ctccacctct tcggccgcgc
     1921 ccgcttcccc gtacgcttcc ccgaagacgg ccagaacgta gcgagcgagc ccggcgtcgc
     1981 attggtctcg cgcgagctcg aaggtataaa tggccgactc gatcaggtgt cgcgcctcga
     2041 cccaattgcc ggccgcggcc gcgagcgtgc cttcgatgcg acgcaagtgg gcggtgaccc
     2101 acggcgccgg atagttctcc atgaagcgct gcgcgcgtcg gagcgcgagc cgcgcggggc
     2161 cttcgtccca gttgacgacg tgggcgagga cccgatgctg caacacctgg cgcatcagga
     2221 acggccagcg cttcgcctcg gtctcggcct cttcgaacgc agcgagcgtc agcttcgtgt
     2281 cggcggagct cgcgagcgct tccattgcgt ccaaataggc gagataggcg cgacagatcc
     2341 cgcgcaggga cggcacctgg caatcgcgca ggcgttgggc caccgcctgg aagccttcga
     2401 cgcgcccgct gagatgcaag acgcgcatcc acgtcggaat cgccgcgagc ggcggcatgc
     2461 cggtttcgtc ggaccaaatc tcgagcaagc tcaaggcctc gagcgattgt tcggtgcgtc
     2521 cgaggcggag cagcgcttcg gcttcgactc gggtgagcag taggtgatgc ttacccatcg
     2581 ccgagcgcgc gtcgttgctc gtatcgcgcg cgcgtcggat gagctccaaa acgctctcgg
     2641 ggttcgcgag gttgtcgaga tcgcactgcg cctggtgcgc gaagcagcgc gctgccaaaa
     2701 tcgggtaccc ggcgatcttg gcgcgctcgg ccagggcctc gaaattgcgc gtcgcggtcg
     2761 tcgccttccc cgcgagcatg ggcctgagcc ccaggaattc ctcgaggtgg agctcgagca
     2821 tgagcggcaa accactgaac ccgacctcgt cgatgcgcgt gaaggcgcgc tccaagagct
     2881 cccggtcacc cgcgcccatt gccgcggaga gcgacgccaa aaaggtcaag cacagcacct
     2941 ccggatcgct ctgcgtgctc aacgactcgc tgcgcgatgc gtaagcgtgc acgtgtccca
     3001 gatcgaacag actcgcgtct tgcatcgagc gcacgagggc agccaccgcg aaagtgcgcg
     3061 ccgcgatctg cgcgtcgagc tcacccgctt ccgcgtcggc gagcgcgctc gagatggcca
     3121 gcaacgctcg cttcgcatcg ttcgcgacga cgatctcgga cagagctagg ccgacggccg
     3181 ctacggcgcg agcgttgggc gagagcgtgc gtcgcgtcgc gagcagatga agcatcgtgc
     3241 tctgcccgcg ccggacgtct ccgcccagaa tgcgttcgag cgcaggcagc gcctcggcaa
     3301 gcccttcggt gctgcgagag ctcgcgagcg gcgcgggtgc cattcgggct ccgggttcga
     3361 cgccgacgcg cgccgggtcg tccgtcctga gccgcagcag acgatcgagc tcctttccgc
     3421 gtgggcggcg tgcctccgct gccccctccg gcagctccca acggtagatc gtgtgcggcg
     3481 tcactcccag ttcggctgcg aatgcagccc gactcagctc gccacggagc gtgcgcacct
     3541 cgctcgggga aagcgaaatc ggccgctccg tctgcgcttc cagcatccct tggatgtaat
     3601 ccgcgacgtg cggagaatcc agccaggccg agcgacaatg gactaaatgg aataatccgg
     3661 ttgtggtttt tggagtaatc cggatgaatg gcgcggatga cgttcagcac ctcggatgcg
     3721 tataagtgat tgaaattgtt gcttggcgcg atcggcacgg gccgtgcagt ggcggtggtc
     3781 actatggtca gcacagcaga acccggaagt ttcgcctctc cccgcattgg tgcagaggag
     3841 ggcgaagtga ggaagccgcg gcgcgttgca ttggtggtgt tgccgctact ctcggccctc
     3901 gcgtttggcg gcggcgcagc gttgttactg gcgagcggcg ggcacgaaac caccgatgac
     3961 gctcaggtcg aaggtcacgt cgcttacgtc gcgccgcacg tcgcaggtca ggtgaagcgg
     4021 gttctggtga aggacaacca gcgcgttcaa gtaggagacg tgctggtcga gttggacgag
     4081 cgcgatctga acgcgaagtt gttggcggcg cgggcagact gggaggcttc gaaggcgcag
     4141 ctgcacgcga gtgagactca acttgcggtc acgcgcaaat cggtgaactc gaacctcgtc
     4201 gtcgcgcgtg gtgggctcgc gcaggccgct tcgacccgtg aaacgacggt agctgccatc
     4261 gagcaggcgc gcgcatcggt ccaagcggcg gagtcgcgtc gcgcgttggc cgccaccgag
     4321 ctcgagcgca ccaagaatct gttcgagact ggtgcgagct ccaaggcgaa cctcgactcg
     4381 gaccgcgctg cgttcgacca agcgcaagcc gctttggccg aggcgagcgc gcgactcgcc
     4441 agcgctgaag ccaatcggca aaactcctcc ggcaccgtcg agtcggcacg cggtcgcgtc
     4501 ttggcggccg aggcggggcc cgagcaaatc gaagccgcgg aggcagccgt cgagctcgct
     4561 cgagcgcggg tgaatcaggc caaggctgcg ctcgaccagg cagagctgaa cgtctcgtac
     4621 acgcgcgtga aggcggaggt cgctggcgtc gtctcgcgac gctcggtgga agcggggcaa
     4681 atggccgcac ccgagcgccc gatgctcgcc ctggttccgc tcgaagatac gtgggtcgtc
     4741 gcgaacttca aagaagacca gatcgcctcg atgcgtcccg gacaaacggt gaacgtgagt
     4801 atcgatgcgt accccggcaa gaagctgagc ggtcacgtcg atagcttggc ggggggtacc
     4861 ggcgctcgct tcgcattgct cccgccggac aacgcgtccg gtaacttcac caaggtcgtg
     4921 cagcgcgttc cggtgttgat tcgcctcgac gcgcatccgg gaatcgagct tcggcccgga
     4981 ctcagcgcgc gcgccaccgt gatcacggag tgacagcgag gcctcatggc ggagcaaatt
     5041 cgtggatcca aggccggcat caccgcggtg gtgatggtgg ccgcgctgat ggcgatgatc
     5101 gacatcacca tcgtgaacgt cgcgctcaac gacatgcgcg cgagcttcgg tacgccgctc
     5161 gaccagatcg gttgg
//
LOCUS       DASTKS010000550         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_86644,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000550 DASTKS010000000
VERSION     DASTKS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86644"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1752
                     /gene="csb2"
                     /locus_tag="VFQ35_11365"
     CDS             148..1752
                     /gene="csb2"
                     /locus_tag="VFQ35_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HET9931282.1"
                     /translation="MLAFEIELLTERYAATAHNDRGRAEWPPHPARFFSALVAALHDD
                     ADIDQAEREALLWLERQNPPALWVDPESCVGRRQVQDVYVPVNDITLGLDADIRSAEQ
                     AVQQAATPAKRKAAEKKFVAARNAPNVVEDDPSDKALKTAIALLPERRTRQVRTFPVV
                     IPETPTFAFLWPAADPSPHRAALERLCARVTRLGHSSSFVRCSVVDRDLTPTLVPSDE
                     GDVVLRVVGPGQLERLEHAFERHQGVESRVLPARPQRYGSAFKVSALPARAESVFSTD
                     WVLFERVGGSRPVASRATDLARALRRALIELHGNQDLPATLSGHAERGPTEQPHVAFV
                     PLPFVENEYADGALMGCALVLPRELPKSERELLLRLVAKWEKERSDQRGNLTLAGGTL
                     PPFMVRRVDVSAKAALDPSRWCRASTRFVTATPIALDKNPGNLRSNQEGTARKAALEA
                     QQSISDACLRVVGARPCSVEVSLAPLLPGAQHVRDFLPWPGRPGRTPRVRVHADIVFA
                     KPVRGPLLLGAGRYFGLGLCLPMEDR"
     gene            1749..>2092
                     /locus_tag="VFQ35_11370"
     CDS             1749..>2092
                     /locus_tag="VFQ35_11370"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9931283.1"
                     /translation="MTLSPSDFGAFHAAVHGGKRPFAWQQRLLDQIVRDKAWPQVLDL
                     PTGAGKTTCIDIALFALALDTSNESAERWCPRRIAMVVDRRIVVDQIAERGRSILNAL
                     MTSADTVVIEVAR"
BASE COUNT          345 a          706 c          684 g          357 t
ORIGIN      
        1 cgcggtcgcc aaacttcctg aggccatcaa gttcgacaag cctgcgggca aagcactcgc
       61 ggagctgcag ccgtcgccga agcttgccga tctcgtgagg aagagccgcg agcttgcggt
      121 cgccgaggct gatgtcggag acgcgtaatg ctggcgttcg agatcgagct cttgacggag
      181 cgctacgcgg cgaccgcgca caacgatcgc ggacgcgccg agtggccgcc gcacccggcg
      241 cgtttcttct cggcgctggt cgccgcgctc cacgacgacg cggacatcga ccaagcggag
      301 cgagaggcgc tcttgtggct cgagcgacag aatccgccgg cgctgtgggt cgatcccgag
      361 tcctgcgtcg ggaggcgcca ggtgcaggac gtctacgtgc cggtgaacga catcacgctc
      421 gggctagacg cggatattcg cagcgccgag caagccgtcc aacaagcagc gacgccggcc
      481 aagcggaagg ccgcggagaa gaagttcgtc gccgccagaa acgccccgaa tgtcgtggag
      541 gatgacccgt ccgacaaggc actcaagacg gcgatcgcgc ttctccccga gcgtcgcaca
      601 cggcaggtgc ggacgttccc ggtcgtcatc cccgagacgc caaccttcgc gttcctctgg
      661 cccgctgccg atccttcgcc gcatcgagcc gcgctcgaac gactctgcgc acgagtgacg
      721 cgacttggac actcttcttc gttcgttcga tgtagcgtcg tcgatcgcga tctcacaccg
      781 acgctcgttc caagcgatga gggtgacgtc gtccttcgcg tggtcggtcc cggccagctt
      841 gagcgcctcg agcacgcctt cgagcgccat caaggtgtcg agagccgcgt gctcccagcg
      901 cgtccgcaac gctacggctc ggcgttcaag gtcagcgcgc ttcctgcaag agcggagagt
      961 gtgttctcga ccgactgggt gctgttcgag cgcgttggcg ggtcacggcc ggtcgcctca
     1021 cgcgcgaccg atctcgcgcg ggcgctacgt cgtgcgctga ttgaactcca cggcaaccaa
     1081 gacctgccgg cgacgctgtc aggacacgcg gaacgcggac cgaccgaaca gccccatgtc
     1141 gcgttcgtcc cactgccgtt cgtcgagaat gagtacgccg acggagcgct catggggtgc
     1201 gcgctggtac taccgcgtga gctccccaaa agcgagcgcg agcttttgct tcgcctcgtc
     1261 gcgaagtggg agaaggagcg ctctgaccaa cgagggaatc tgactctcgc tggtgggacc
     1321 ctcccgccgt tcatggttcg ccgagtggac gtgtcggcga aagccgccct cgatccgtcg
     1381 cggtggtgcc gcgcctccac ccgcttcgtg accgcgacgc cgatcgccct cgacaagaat
     1441 cccggcaacc tgcgcagtaa ccaggagggc accgcacgca aggccgcgct agaggcgcag
     1501 cagagcatta gcgatgcgtg cctccgcgtt gtcggggctc gtccctgttc ggtggaggtg
     1561 tcactcgcgc cgcttctccc gggcgcacag cacgtgcgtg atttcctgcc gtggccaggc
     1621 cgtccaggtc gaacgccccg cgtgcgggtc cacgccgaca tagttttcgc gaagcccgta
     1681 cgaggcccgc tcttgctcgg cgcagggcgc tacttcggcc tgggtctctg cttacccatg
     1741 gaggaccgat gacgctctcc ccttccgact tcggagcctt tcacgcagcc gttcatggcg
     1801 gcaagcggcc cttcgcttgg cagcagcgcc tgctcgatca gattgttcga gataaggcgt
     1861 ggccgcaggt gctcgacctg ccgaccggcg cgggaaagac gacatgcatc gacatcgcct
     1921 tgttcgcgct cgctctcgac acgagcaacg agagcgccga gcgttggtgc ccgcgccgca
     1981 tcgcgatggt ggtggaccgc cgcatcgtcg tcgatcagat cgcggagcgc gggcgctcca
     2041 tcttgaatgc cttgatgacg agcgcagata cggtcgtcat tgaggtcgct cg
//
LOCUS       DASTKS010000551         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_309480, whole genome shotgun sequence.
ACCESSION   DASTKS010000551 DASTKS010000000
VERSION     DASTKS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309480"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1063)
                     /locus_tag="VFQ35_11375"
     CDS             complement(38..1063)
                     /locus_tag="VFQ35_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9931284.1"
                     /translation="MKRVLVLWLVMLAAAIVAACVEREPARFPHQAHLTTQGCTGDCL
                     SCNTCHTPSAKGRERKLPPATICATCHRNDAARATAVLRVSPPRPTGSIVFDHAQHLA
                     MPAIQGQCVGCHAGVVEPERPSIPTMSQCFTCHEHEQQWQRAECTPCHDQRDLKASLP
                     RTFLRHDENFARRHATPATTQKQLCQSCHTQADCDGCHDAVQGLSIERRMPERIERTF
                     VHRGDFLSVHAIEARAEPAKCQRCHEASSCEGCHTARGVSGGLINGRNPHPLEWVRAN
                     PQALNFHGRAAKRDILSCAGCHDQGPATNCIRCHRVGGYGGNPHPNGRWPSGGGLNQT
                     MCGYCHE"
     gene            complement(1060..2436)
                     /locus_tag="VFQ35_11380"
     CDS             complement(1060..2436)
                     /locus_tag="VFQ35_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931285.1"
                     /translation="MNQSALLLIALAFSVSETAAAEVPSAEREDRFTFLTHSETHAQL
                     FRRALLPGTNGTTVATETALPITQYVTLSARDIDAPWRRDSVDIEVALWGRVHLYDLT
                     SQDRFDGDVQSANVRQRFGKFALRLGRQFVAGGAARYARFDGGDLTVDLGAGFWAEGY
                     AGFTAMPRWNERPSYLYLGTASDSGVRSLASTPAAARDSYWQAGARVGFMHVRGDAVV
                     SFHEQHEALGLAHRNLGANARYMIADKATLGGTAILDLDTNRFADASLFVDYFFSRKV
                     DASLEYFHLEPSLLLSRQSVLSVFTTDAYDEFGGSVRVRPTSSLTLQGLGFANGYSDG
                     EVGGRTELSASAWVDDRRLTFVRLAYARVLAPDNGYNSARASVGRKFGRWLMSTVEAY
                     YYLYDREVRGARSSSVYAATLTYRPSPSVSLLGGASLARTADANVDAQGQLRLSCDFD
                     VVRHARGL"
     gene            2843..3562
                     /locus_tag="VFQ35_11385"
     CDS             2843..3562
                     /locus_tag="VFQ35_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9931286.1"
                     /translation="MKPPKLATPAEWLQHRRLLLEKEKELSRLQDALARQRKELPWVR
                     LEKNYEFDGAAGKVRLSDLFSGKSQLIIYHFMFGPGWSEGCPSCSLMSDQFDATLCHL
                     AARDVAFAVVSRAPYAEIAPFKKRMGWSFLWVSSFETDFNRDYNVSFSREEVKGGKQF
                     YNFGPNGFPSEEGPGISVFAKDDEGALYHTYSTYARGAEPMLNVYQFLDITPKGRAEE
                     GLPWPMAWVRHHDRYEGPSAA"
BASE COUNT          661 a         1150 c         1192 g          639 t
ORIGIN      
        1 gagcgcccac agcgcacgag ctcgggacgg ctcgaggtca ctcatggcaa tacccgcaca
       61 tggtttggtt gagtccgcct ccgctcggcc agcgcccgtt cggatgcgga ttgccgccgt
      121 agccgccgac gcgatgacaa cggatgcagt tcgtagcggg gccttgatcg tggcagcccg
      181 cgcaagaaag gatgtctcgc ttcgccgctc gcccgtggaa attcaaagcc tgcgggttcg
      241 cgcgcaccca ctcgagcgga tgcgggttgc gcccgttgat gaggccgccg ctcactccgc
      301 gagccgtatg gcagccttca cagctactcg cctcgtgaca gcgttggcac ttggccggtt
      361 cggcgcgagc ctcgatcgcg tggacggaca agaagtcgcc gcggtggacg aacgttcgct
      421 cgatgcgctc tggcatgcgc cgctcgatgg aaagtccttg caccgcgtcg tgacagccgt
      481 cgcagtcggc ttgggtatgg cacgactgac atagctgttt ctgggtcgtc gcaggcgtcg
      541 cgtggcgtcg cgcgaaattc tcgtcgtgtc gcagaaacgt gcgcgggagg gaggccttga
      601 gatcacgctg atcgtgacac ggcgtgcact cggcccgctg ccactgctgt tcgtgctcgt
      661 gacaggtgaa acactgactc atcgtgggaa tgctcgggcg ctcgggctcc accacgcccg
      721 catggcaacc gacgcactgg ccctgaatcg cgggcatcgc caagtgctgg gcgtggtcga
      781 acacgatgga gccggtcggt cggggcgggg acacccgcag caccgcagtc gctcgcgccg
      841 cgtcgttacg atgacaggtc gcgcaaatgg tcgcgggggg aagcttgcgc tcacgccctt
      901 ttgccgaagg cgtgtggcaa gtgttgcagc tcaggcaatc gcccgtgcaa ccctgggtcg
      961 tgaggtgagc ctggtgcgga aagcgcgcgg gctcgcgctc gacgcacgcg gccacgatcg
     1021 ccgccgcgag catgaccagc cacagcacga gaacccgttt catagccccc tcgcgtgccg
     1081 aacgacgtcg aagtcgcacg acagccgcag ctgtccctgg gcgtcgacgt tggcgtccgc
     1141 ggttcgcgcg agcgacgcgc cgccgagcaa gctgacgctg gggctggggc gataggtgag
     1201 cgtcgctgcg tacacggacg agctgcgcgc gccgcgcacc tcacgatcgt aaaggtagta
     1261 gtaggcctct accgtgctca tcagccagcg cccgaatttc cgtcccaccg acgcgcgcgc
     1321 cgaattgtaa ccgttgtcgg gggcgagtac tcgagcgtag gcgaggcgca cgaacgtgag
     1381 ccgccggtcg tcgacccagg cgctcgcgct gagctcggtt ctgcctccga cttcaccgtc
     1441 cgagtacccg ttcgcaaatc cgagcccttg taatgtcaga ctcgaggtcg gtcgcacccg
     1501 gacggagccg ccgaactcgt cgtaagcgtc ggtggtgaac acggacaaca ccgactgtcg
     1561 cgacaggagc agagaaggct ccaggtggaa gtactcgagc gaagcgtcga ctttgcgtga
     1621 gaagaagtag tcgacgaaca acgaggcgtc ggcgaagcga ttcgtgtcaa ggtcgaggat
     1681 cgcggtcccc cccaaggtcg ccttgtcggc gatcatgtag cgagcgttgg ctcccaagtt
     1741 gcgatgagcg agcccgagcg cctcgtgctg ctcgtggaag gagacgaccg cgtcgccgcg
     1801 cacgtgcatg aagccgacgc gtgcacctgc ttgccaatac gagtcgcggg ccgcggccgg
     1861 cgtcgacgcc agactgcgga cgcccgagtc cgacgccgtg ccgaggtaca ggtagctcgg
     1921 tcgctcattc cagcgcggca tcgcggtgaa gcccgcgtat ccttcagccc aaaatccagc
     1981 tccgaggtcg acggtgagat ctccgccgtc gaaacgggca tagcgagcgg cgccgccggc
     2041 cacgaactgg cgacccagtc gaagcgcgaa cttgccgaaa cgctggcgaa cgttcgcgct
     2101 ctgaacgtct ccgtcgaacc gatcttggct cgtcaagtcg tacagatgaa cacgccccca
     2161 cagtgcaacc tcgatatcga cgctgtcgcg ccgccacggc gcgtcgatgt cgcgcgccga
     2221 aagggtcacg tattgcgtga tgggaagcgc ggtctcggtg gcaacggtgg tgccattcgt
     2281 gcccggtagc agcgcgcgcc ggaagagctg cgcgtgcgtc tcactatggg tcaggaacgt
     2341 aaaacgatct tcgcgctctg cactcggcac ttcggcagcg gcagtctcac tgacgctgaa
     2401 cgccaacgcg attagcagca acgcgctttg gttcaccgtt tcgcattact actcccgttg
     2461 tcacgccgag atgaacccaa attcaattgg caatgagcct ggtcagttcc cgccgtgggc
     2521 agcgtcgagc ggttgacacg gcgagtcctg gctgcggccg tcgagccgac ggggccggcg
     2581 tccgacgaag tgcgcatttt tcaagaaatc ggtgggggct tgtcgaaccg agcggggctc
     2641 gcccgacatc ggttggaaag catgaaaacg gaggctccca tgtgtccggc ctgtctcgct
     2701 gcgagcgcag tcatcgcgct caaagcggct tccgcgggcg gcgctgccgc gctcgccgtg
     2761 aagacgctgc gctccagatt cggcagttct tcaaccgagt ctgacccaac taccgccacc
     2821 gcttccgaag gagaacccct caatgaaacc gcccaaactc gcaaccccgg ctgaatggct
     2881 ccaacatcgt cgcctcctcc tcgaaaaaga gaaggagctg agccgtctgc aagatgcgct
     2941 cgcgcgccaa cgaaaggagc tcccctgggt tcgactcgaa aaaaactacg agttcgacgg
     3001 ggccgccggg aaggtgcgtc tctccgacct cttctcgggc aaaagccagc tcatcattta
     3061 tcacttcatg ttcgggccgg gttggagcga gggctgcccg agctgctcgt tgatgtcgga
     3121 ccagttcgac gccacacttt gccacctcgc ggcgcgcgac gtggccttcg cggtggtctc
     3181 ccgcgcgccg tatgccgaaa tcgcgccgtt caagaagcgc atggggtgga gctttctttg
     3241 ggtttcgtcc ttcgagaccg acttcaatcg tgactacaat gtctcgtttt cgcgcgagga
     3301 agtgaagggc gggaagcagt tctacaactt cgggccaaac gggtttccga gcgaggaggg
     3361 ccctggaatc agcgtgttcg cgaaagacga cgagggcgcc ctctatcaca cttactcgac
     3421 gtacgcgcgc ggagcggagc cgatgttgaa cgtctatcag tttctggaca tcacgccgaa
     3481 ggggcgggct gaggaaggtc tgccttggcc gatggcgtgg gtgcgccatc acgaccgcta
     3541 cgaagggcct tccgcggctt gaccatggac gcgccgcggt gctgaagtcg tgccgcggcg
     3601 cattttcgag ggttcgatgc gatacgtaat gttgatatac ga
//
LOCUS       DASTKS010000552         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1872692, whole genome shotgun sequence.
ACCESSION   DASTKS010000552 DASTKS010000000
VERSION     DASTKS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872692"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFQ35_11390"
     CDS             complement(<1..280)
                     /locus_tag="VFQ35_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9931287.1"
                     /translation="MAQRQKDEIRKRLLSAALEVFSEVGFAAATVAEVAARAQVSTGN
                     VYRYFASKEVLLREALPATFVSELKAMTRARVDALGAVRDIARLPADAE"
     gene            complement(994..1848)
                     /locus_tag="VFQ35_11395"
     CDS             complement(994..1848)
                     /locus_tag="VFQ35_11395"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET9931288.1"
                     /translation="MSPRPVVALLESRMSRELARLVEKHGGDSVGVPAVKECPEFTPE
                     LANKLIDELSGDRHEVVVFMTGVAVSLLFEMAEQIGRRPDLVAALRRLTTVCRGPKPT
                     AALRGFGVPPTLSAREPFTSADVIDALSGIELKGRRVILLHYGERSETLAETLLARQA
                     RVEELWLYRWLMPDDTTGLANIVKRIIAGDVDALAITCQIQFRHLHEVAARAGLAEKM
                     VRALNDRVVVAAVGPTCEAILHAYGVRVHVMPDHPKMGPLVVALMRYLERGRSTDSSS
                     AELAPVSN"
     gene            complement(1859..>3433)
                     /locus_tag="VFQ35_11400"
     CDS             complement(1859..>3433)
                     /locus_tag="VFQ35_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931289.1"
                     /translation="GLIRASLLETPIRTGASNESETSAPEHLLALAEVGRHLTSGGDI
                     PDAVARALRVLDRRLAALRSVLSIADSERRTLEIEATHGLAPEQFRLRFGQGVAGRVS
                     ETGRPIVVPVLRHEPMALSELSDLAAWSDGHWSVVSVPVTMAGRHVGALSAYFPRVSG
                     EDFSGRLGVLEIAASLIGQALRAARLEGLNDEGSVDEAEQRSQRALFEYSNMIGASAA
                     MRQVYEQVGQVARTNATALIRGESGTGKELVAHAIHNNSMRARQPFVRVNCAALPETL
                     FESELFGHERGAFTGAHARRKGRFELAQGGTLFLDEIGELSPTTQAKLLRVLQSREFE
                     RLGGSETLRTDVRIVAATNKDMERAVSAGTFREDLYYRLNVFTIALPALRDRRADIPS
                     LAEYFLSKFAGEHRRKIARVSSGAMEVLAQYSWPGNVRELENVIERAVVVCDGFVIQE
                     HHLPDSILSATRDRPVERLTLADAVARLERQMIEEALRHARGNCARAARALGTTERIV
                     RYKAGKYRIDTTRFRE"
BASE COUNT          609 a         1154 c         1107 g          563 t
ORIGIN      
        1 actctgcgtc cgccggtaac ctagcaatgt cgcgcacggc tccgagcgca tccaccctcg
       61 ctcgcgtcat cgctttgagc tccgacacga acgtcgccgg caacgcctcc cgcagcaaca
      121 cctccttgct cgcgaagtag cgatacacat ttcccgtcga cacttgcgcg cgcgcggcca
      181 cctccgccac cgtcgcggcc gcaaagccca cttccgaaaa cacctcgagc gctgccgaca
      241 acaagcgctt acgaatctcg tctttctgtc gctgagccac tcaaaagcga atattgaatt
      301 cacttttgag cccgtcaagc cgccatcact cagaccgcgc aaatttgctc agcaaattcc
      361 ggtagctggc ttccgaatgg gggcctcacc gaaaagagcg cgaaagccga ccccgagcgc
      421 ggcaggcccc ctctggcgtc gtctcacggg tccggtgccg cgcccgcccc acgccgtttg
      481 gtcgccaccg atcctgccaa cccgtcgata cggactccac gctaggtacc gcgcggaagc
      541 tcaattctac gtggacctgt gtgtgcgcgc ctgcgatccg cggttcgcca cccgggccgc
      601 tcgactgatc tcacgattca cgagccattg ttcaaaaccg tcccggaagt gagatccagg
      661 cgacgggagc gcaggttgac cggcgagcgt caacacaaac gacgccgcga tgcgtagtcg
      721 ttgagcgggc atgttgcgcc gccccgccgc cccaagtcga cctcgcagtg ccgccgcgag
      781 cgccccctca aggccgacaa cttctacgag tgctttgccg cgaatgtccg gacatccgct
      841 gatccgaatg cgccaccggg tctccgccgt tccgacgaag acatagagtt cttcaagttt
      901 cctcttttgg tgatggattc ggtctccgca atgtgcgccg cggcccgccg tgcccttcgt
      961 gacgccgctc cctccaaccg gaaattccga ccttcagttg ctgacggggg cgagttcggc
     1021 cgagctcgag tcggtgctgc gtccgcgttc gaggtagcgc atgagcgcga ccacgagggg
     1081 gcccatcttc gggtggtcgg gcatgacgtg gacgcgcaca ccgtaagcgt ggaggatggc
     1141 ctcgcaggtt ggaccgacgg cggcgacgac gacgcgatcg ttgagagctc gcaccatctt
     1201 ctcggcgagg cctgcccgcg cagcgacttc gtgcaagtgt cggaattgga tttggcaggt
     1261 gatggcgagc gcgtcgacgt cgcccgcgat gatgcgcttg acgatgttgg cgagaccggt
     1321 ggtgtcgtcg ggcatgagcc agcggtagag ccagagctct tcgacgcgcg cctgccgggc
     1381 gagcagcgtc tcggcgaggg tctcgctgcg ttcgccgtag tgaaggagaa tgacgcgacg
     1441 gcccttcagc tcgatgccgg agagagcgtc gatgacgtcg gcggacgtga acggctcgcg
     1501 cgcgctgagg gtgggcggca caccgaagcc gcgcaacgcc gccgtcggct tggggcctcg
     1561 gcacacggtg gtgaggcgac gcagcgccgc gacgagatcc gggcgccggc caatctgttc
     1621 ggccatctcg aacaagagag agacggcgac accggtcatg aacaccacga cttcgtggcg
     1681 atcgcccgag agctcgtcga tgagcttgtt cgcgagctcg ggcgtgaact cggggcactc
     1741 tttgacggcc gggacaccga cggaatcgcc gccgtgcttc tcgaccaaac gagctagttc
     1801 acggctcatg cgcgactcga gcagggcaac gacggggcga ggtgacatga cgtttcagct
     1861 actcacgaaa gcgagtggta tcgatgcggt acttgccggc cttgtagcgc acgatgcgct
     1921 cggtggtgcc gagggcgcgc gcagcacgag cgcagttgcc gcgagcgtgt cgtagcgctt
     1981 cttcgatcat ctggcgctcg aggcgagcca ccgcgtcggc caaggtgaga cgttcgacgg
     2041 ggcggtcacg cgtcgcggac agaatcgagt cggggagatg atgctcctga atgacgaagc
     2101 cgtcgcaaac gacgaccgct cgctcgatga cgttctcgag ctcacgcacg ttgccgggcc
     2161 acgaatactg cgctagcacc tccatcgcgc cgctcgacac gcgcgcgatt tttcggcgat
     2221 gctcgccggc aaacttgctg aggaagtact cggcgagaga cgggatgtcg gcgcgccgat
     2281 cgcgcagcgc cgggagcgcg atcgtgaaca cgttcaggcg gtagtagagg tcctcgcgaa
     2341 acgtgccggc gctgaccgcg cgctccatgt ccttgttcgt ggccgcgacg atgcggacgt
     2401 cggtgcgcaa cgtctcactg ccgcccaggc gctcgaactc gcgcgactgc agcacgcgca
     2461 ggagcttcgc ctgcgtagtc ggagagagct cgccgatctc gtcgaggaac aaggtgccgc
     2521 cttgagcgag ctcgaagcgc cctttgcgcc gagcgtgcgc gcccgtgaac gcgccccgct
     2581 cgtgaccgaa cagctccgac tcgaagagcg tctccgggag cgccgcgcag ttgacgcgca
     2641 cgaacggttg ccgcgcgcgc atcgagttgt tgtgaatcgc gtgcgcgacg agctccttgc
     2701 cggtgccaga ctcaccgcgg atcagcgccg ttgcgttggt ccgcgcgacc tgcccgacct
     2761 gctcgtagac ttggcgcatg gccgcgctcg cgccgatcat gttcgagtac tcgaacagcg
     2821 cgcgctgaga gcgttgctct gcctcgtcca ccgagccctc gtcgttcagg ccctcgaggc
     2881 gagcggctcg cagcgcttga ccgatgagcg acgccgcgat ctcgaggact ccgaggcgcc
     2941 cgctgaaatc ttcgcccgaa acgcggggaa agtaggccga aagcgcgccc acgtggcgcc
     3001 ccgccatggt taccggcacg ctgacgacgc tccaatgccc gtccgaccac gccgccaaat
     3061 ccgagagctc cgaaagagcc atcggctcgt gcctcaacac cgggacgacg attggacgcc
     3121 cagtctcgga aactcgaccg gcgacaccct gtccaaaccg gaggcggaac tgctccggag
     3181 cgagcccgtg cgtcgcttcg atctcgagag tacggcgctc cgagtccgca atcgagagaa
     3241 cggagcgcaa agccgcgagg cgccgatcga gcacacgcag cgctcgcgcc accgcgtccg
     3301 gaatgtcacc acccgacgta aggtgccgcc ccacctcagc gagagccaac aggtgttccg
     3361 gggcggacgt ttccgattcg ttgcttgcgc cggtgcgaat tggggtctcg agcagggagg
     3421 ctctaatcaa gcc
//
LOCUS       DASTKS010000553         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_310018, whole genome shotgun sequence.
ACCESSION   DASTKS010000553 DASTKS010000000
VERSION     DASTKS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310018"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFQ35_11405"
     CDS             complement(<1..467)
                     /locus_tag="VFQ35_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931290.1"
                     /translation="MRRFLRAVPLLACIACSGGSADPANTPTGGATSAGGQGGLTGGS
                     GGVSGATAMGGVGVGGGAAGAGAQCSNASVDPGAVAVGASLPARFVLVTDPALDDASA
                     IASWTDFTADCGSGAVGPLNQKGFAPWGECVARHYVERVNETFASLLQTKSPLL"
     gene            complement(1293..1934)
                     /locus_tag="VFQ35_11410"
     CDS             complement(1293..1934)
                     /locus_tag="VFQ35_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9931291.1"
                     /translation="MLELFYYPSNASLAPHVLLEELGASYELRLVDRDTGALDSPAYR
                     RLNPSGLIPALRDGELVLFESAAICLHLCDTHPDAGFAPHLGTPERAHFYKWLSWLSS
                     TLQPTILLGHYAARWCSDSTAASELRTKAESRASELLSLADAELARHGGPFLLGERYT
                     ALDAYALMLCRWSRNFARPARRMQSLGPYVERLLERPATIRAFEQERLPKPWA"
     gene            complement(2056..>2578)
                     /locus_tag="VFQ35_11415"
     CDS             complement(2056..>2578)
                     /locus_tag="VFQ35_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931292.1"
                     /translation="EAWRFSRNEHRLATLSKSGLVVWDTESGNKWGISAPPGLGRVRS
                     LDFANDDEVVVLRQANGDAIAFRYASNERVPPPALAFEDGAAVQWSLASHFLSAFRRS
                     DGAWLGAVGLLGGGSSGVVVSAGGELDFIGAEPVPQPRCAFGNVLAAWPVCSDTQLVP
                     GLLKRLLRDAGPR"
BASE COUNT          470 a          837 c          852 g          419 t
ORIGIN      
        1 agcagcggtg acttcgtctg cagcagcgac gcgaatgttt cgttcacgcg ctcgacatag
       61 tggcgggcca cacactctcc ccaaggggcg aagcccttct ggttcaaggg gcctaccgcg
      121 ccactgccgc aatccgccgt aaagtcggtc caggaagcga tcgcagaggc gtcgtccagc
      181 gccggatccg tcaccagcac gaagcgtgca ggcaacgatg cgccaacggc caccgcgccc
      241 ggatccacac ttgcgttcga gcactgcgcg ccggctccgg ctgctccccc gcctacgccg
      301 acgccaccca tggccgttgc gccgctcact ccccccgaac caccggtgag accgccttgt
      361 ccgcccgcgc tcgtcgcgcc acccgttggc gtattggctg gatcagcaga acctccagaa
      421 caggcaatgc aggccaagag cggaacagca cgcaagaatc gacgcatagc cgattcctac
      481 ccaaaccgag cttgaaggag aagccgttga ctttggcgcg cagtgcggtt tggagccgtc
      541 cagtcagaca ccgtccgccg cgggttacga tgagcgaacc ggcaggcgaa caggtacctg
      601 gggatctcgc ggcgagtctc ctggtgattc tctggatcgg aatagcgtcc tcttccggat
      661 cgagggcaat tgaaccacga ccgaacataa tgcctattgc ctggccacca cgaagcgcgc
      721 agagtcgacg gcgagcttcc gtgatcatcg gcaggcgggg ctcgggaggc gtatatcgat
      781 gattgggcga ctcgttttcg tcggggcgtt ggccgtgacg ttcgccggat gcgcggtgga
      841 tgccacgggc actgagtcgg acaagtctga agatacagcg gggtcgcaag cgacgctgcg
      901 aattaacccg gacaatcccg ggccgcggcc cgcgacgcct tgcgcgagag aatgtacccg
      961 tcgctacgcg agtgacatca tgatctgcgt caaccttgcg gatccgggtg atgatgcgga
     1021 caagtgctcg acggatgcgc tcgcccgcta cggcaattgt ctaacgggat gcacctaagg
     1081 ggcccgtgaa tcggtaagtc ataccgcgcg agccgatcct cttgctgcgg acgggctcgt
     1141 cgctgcgcta cttgcccaat cttagtaggc gtccggtgct cacccctcac gcgcctgcct
     1201 cgctgcggtc tcggcttgct cgcgactgac ttacccattc gcgggcccta accgcgttag
     1261 cgagaggcac tctgaatggg ttgccttccg aactaggccc aaggcttggg caagcgctcc
     1321 tgctcgaagg cacgaatggt cgcgggtcgc tccaacaagc gctccacgta cggcccgagc
     1381 gattgcatgc gccgcgcggg gcgcgcgaag ttgcggctcc agcggcacag catgagcgcg
     1441 taggcgtcga gcgcagtgta tctctcgcca agaaggaacg ggccgccatg gcgagcgagt
     1501 tcggcgtctg cgagactcaa gagttcgctc gcgcgtgact cggccttggt ccggagctcg
     1561 gacgcggccg tgctgtcact gcaccagcgc gccgcgtagt gtccgagcag gatagtgggc
     1621 tgaagcgtgc tcgagagcca cgacaaccac ttgtagaagt gagctcgctc tggcgtgccg
     1681 agatgcggcg cgaagccggc gtccggatgc gtatcgcaga gatgaaggca gatggcggcc
     1741 gactcgaaca ggacgagctc cccgtcgcgc agcgccggaa tgagcccgct cggattcaac
     1801 ctccgataag cgggcgagtc gagcgccccg gtatcgcgat cgaccaagcg gagctcgtag
     1861 cttgcgccca gctcttcgag gagaacgtgc ggtgcgaggc ttgcgttgga aggatagtag
     1921 aagagctcca gcatcgcgcg ctcggtacca ctcgcagaag ctcgcgtcga tgtgtaagga
     1981 gcggtgggca atagcgcccg tactcgtggc tagtaacccc catcgcaggc ctcgggttac
     2041 gtcgagaccg ctcgctcagc gtggccccgc atcgcgtagt aggcgcttca ggagtccggg
     2101 cacgagctgg gtgtcgctgc agacgggcca cgcggcgagc acgttgccga aggcgcagcg
     2161 cggttgaggc accggctcgg caccgatgaa gtcgagctcg ccgccggcgc tcacgacgac
     2221 gccggacgag ccgcctccga gtaacccaac cgcaccgagc cacgcgccgt ccgaacgtcg
     2281 aaaagcactc aggaagtgcg acgcgagcga ccactgcacg gctgcgccgt cttcgaacgc
     2341 gagcgctggc ggcggcactc gttcgttgct tgcgtagcgg aaagcgatcg cgtcgccgtt
     2401 ggcttggcgg agcaccacga cttcgtcgtc gttggcgaag tcgagcgagc gcacgcggcc
     2461 gagccccggc ggtgcggaga tgccccactt gttgccgctc tcggtgtccc acaccacgag
     2521 gccggacttg ctcagcgtcg cgaggcgatg ctcgtttcgg gagaagcgcc aggcctcg
//
LOCUS       DASTKS010000554         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_756183, whole genome shotgun sequence.
ACCESSION   DASTKS010000554 DASTKS010000000
VERSION     DASTKS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756183"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFQ35_11420"
     CDS             <1..373
                     /locus_tag="VFQ35_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931293.1"
                     /translation="FLASGGAFIALGNLCTALIGSNIVGFELGALLLGTGVSSMSSGA
                     LRYLATLYGSAEDAEANQAAVSLLTNVGVLLGGSLWGAIVESAIVPASVVRLGLLSMT
                     GAVVPMSFGVLLLRTEPARRI"
     gene            451..1596
                     /locus_tag="VFQ35_11425"
     CDS             451..1596
                     /locus_tag="VFQ35_11425"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET9931294.1"
                     /translation="MSSLEVRVPVKPKRRSLLDASDAGPVSLHRRRGGNAELAALKET
                     LRDAGIFSPVPGYYSRKLCEVLLLWGVSVAALLLFPRSLFVLGVGPLLALTTGQTVLL
                     AHDVVHGAAFTSRNVWIAGLRRTLPHVLIGLFSGGSASWWKQSHNAHHVLSNDAERDP
                     DIDYPFLAFDIEQARDKAAFFHPVLRNQHVLVWFMLPAVAITMRLYSVAFLLRRLTRP
                     GHHRLRRALELVVLVAHWILYLGLMSTLPPLVAVAIVAMHQAGFAVYLALITASNHWA
                     MPMPNAEQLSFVEHQVTTSRNIRGGALVQFFYGGLDAQIEHHLFTGLPRPRLLQARPF
                     IRAFCQQRGIPYAEEGPVEAIKLVYQRLRSVAFEVRALDWACLEEVD"
     gene            1597..2889
                     /locus_tag="VFQ35_11430"
     CDS             1597..2889
                     /locus_tag="VFQ35_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HET9931295.1"
                     /translation="MVPERPGWPTQGTSRLLGFLRDRGLPFLSGADDAKLRTLALGMR
                     AAARRTMAGPLPPGTARRGDGAIFATLGLEDRVRVLTDNRGAFLEKCELVEAARQSVD
                     CALFYLADDATGERFAHALEQAAARGVKVRLCADAYASVEKQYGPFGYALPASRGSLA
                     LVERLRERGCEVHLMGTDHWCMHRKFLFVDRSQLVLGGRNVADHYAEPGWRDLELFLQ
                     GPFARSFTALVDGTFAAPTEEPACVPGVLAGVPGGAGRAFERAVSRLVDEARTSIDIE
                     HAYLLSQPWLVERLRAAVNRGVRARAFTNGPRSNDLPFMNWRLAVSLRELLDAGVHVF
                     RCAGVNTLHTKLIVADERKIVFGSTNLDYYSPTYCAELDIAIESASLGAELASIIEAG
                     LALSDSEAVLPGTGTSLERECRGWSVSRVFDLLLHDIQ"
     gene            2886..>4103
                     /locus_tag="VFQ35_11435"
     CDS             2886..>4103
                     /locus_tag="VFQ35_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9931296.1"
                     /translation="MTLHPPFEPGQLLIAEALSAALGPISVPELERILLSVRLSRFAA
                     GTLVRFDAAAELRVVVAGIVAVHDLGGADETKYLPGQLLPSDAHSAPYRALRDVLVAH
                     VPSEALEGWGADRVLRALARTGLRAHSKPPLLVGISGTDPELVAELHRALAAALARFG
                     SVSVGVVERSRGALPSRLELSGLLAPAEFQVVALPSPVRPSDTPEVDRWLYVVDGQRP
                     GEGCAMPRRDAFERPGAVRLSTAVPRATDALGRAPCVLGEPGLMPRTLVVCHSGSRRL
                     PSATRRHLAFHEPDDHRHVRRGDVGHVERLARELAGVPVGFTFGAGGARGLAHLGVLC
                     AAEEAGVPVDYISGTSIGAVIGALVAMGHDADARREAAERLASARPFWDFTLPTTALL
                     RGRRFEALADEFFG"
BASE COUNT          563 a         1335 c         1373 g          832 t
ORIGIN      
        1 gttcctagcg agcggcggcg ccttcattgc gctcggcaat ctctgcacgg cgttaatcgg
       61 ttcgaacatc gtaggtttcg agctcggcgc gttgctgctc ggaaccggag tgtcgtcgat
      121 gtcgagcggt gcactgcgct acctcgcgac gctctatggt tcggcggaag atgcggaggc
      181 gaaccaagcc gctgtatcgt tgctgaccaa cgtgggtgta ctcctcgggg gttcgctttg
      241 gggcgccatc gtcgagtccg cgatcgtgcc tgcgagcgtc gtgcggctcg ggctactttc
      301 gatgacgggc gcggtagttc cgatgtcgtt tggggtgctc ttgctgcgca ccgaaccagc
      361 gcgccgcatc taacccggcc gatatgcagg cgacggggca tgagcattgt gtcgctggtc
      421 ggagtcgggt catgatgagg gttccccctc gtgtcatctc tcgaagtccg cgttcccgtg
      481 aagcccaagc gccgttcctt actcgacgca tccgacgccg gccccgtgtc gctgcatcga
      541 cggcgtggag gcaacgccga gctcgccgcg ttgaaggaga cgttacggga cgctggcatt
      601 ttctcgccgg tgcctggcta ctacagccgc aagctgtgcg aagtgctgct gctctggggc
      661 gtctctgtcg ccgccctttt gctcttcccg cgctcgcttt tcgtgctcgg cgtggggccg
      721 ctcttggcgc tcacgacggg gcaaacggtg ctgctcgctc acgacgtcgt gcatggcgcg
      781 gcgttcactt cgcgcaacgt ttggatcgcg gggctgcggc gaactctccc tcacgttctc
      841 atcgggctct tctcgggcgg cagcgcgtca tggtggaagc agagccacaa cgctcaccac
      901 gtgctctcga acgatgccga gcgtgacccc gacatcgact acccattttt ggcgttcgac
      961 atcgaacagg cgagagacaa ggctgcgttt ttccaccccg tgctccgaaa tcagcatgtc
     1021 ttggtgtggt tcatgttgcc tgcggttgcc atcaccatgc gcctctacag cgtcgcgttc
     1081 ctgctgcggc gcttgacacg tccgggccat caccggctgc gtcgtgcgct cgagctcgtg
     1141 gtgctcgtcg cgcactggat cctctacttg gggctcatgt ccacgcttcc tccgctcgtc
     1201 gcggtcgcca tcgtcgcgat gcaccaagct ggctttgcgg tctatctcgc gctcatcacg
     1261 gccagtaacc attgggcgat gccgatgccg aacgccgagc agctgtcgtt cgtcgaacat
     1321 caggtcacga cttcacgcaa cattcgcggg ggcgcgctcg tgcagttttt ttacggcggc
     1381 ctcgacgcgc agatcgagca ccatctcttc acggggctcc cgcgtccgcg cttgctgcag
     1441 gcacggccct tcattcgcgc gttttgccaa caacgaggca ttccttacgc cgaggagggt
     1501 ccggtcgagg cgatcaagct cgtataccag cgcctccgct cggttgcgtt cgaggtccgc
     1561 gctctcgatt gggcttgcct cgaggaggtg gactgagtgg tgcccgagcg cccaggctgg
     1621 ccgacgcagg ggacatcgcg tttgctcggt ttcctgcgcg accgtggctt gcctttcttg
     1681 agcggcgcgg atgacgcaaa gctccgaacg ctcgcgctcg gaatgcgtgc cgccgcgcgc
     1741 cggaccatgg ccggaccatt gccaccaggc acggcgaggc gaggggacgg agcgatcttc
     1801 gcgacgctcg gtctcgaaga tcgggtgcgg gtgctgaccg acaatcgggg tgcatttctc
     1861 gagaagtgcg agctcgtgga ggcagctcga cagagcgtcg attgcgcgct gttctacctc
     1921 gccgacgacg cgaccggtga acggttcgca cacgcgctcg agcaagcagc tgcccgcgga
     1981 gtcaaggttc gcctgtgcgc tgacgcttac gcgagcgtgg agaagcagta cggccctttt
     2041 gggtacgctt tgcctgcctc gcgaggctcg ctcgcgttgg tggagcggct ccgcgagcgc
     2101 ggttgcgagg tgcacctgat gggcactgac cattggtgca tgcaccgcaa gtttctgttc
     2161 gtggatcgtt cgcagctagt actcggcggt cgaaacgtcg cagatcacta cgcggaaccc
     2221 ggttggcgcg acctcgagct ctttcttcag ggcccgtttg cgcggtcgtt cacggcgctc
     2281 gtcgatggca cgttcgcggc gcccacggaa gagcccgctt gcgtgcccgg tgtgctcgca
     2341 ggcgtgccgg gtggcgccgg gcgcgccttc gaacgagctg tttctcgctt ggtggacgag
     2401 gcgcgaacca gcatagacat cgagcatgca tacctgcttt ctcagccatg gctggtcgag
     2461 cgccttcgcg ccgcggtgaa ccgtggcgtg cgagctcgag cgtttaccaa cggacctcgg
     2521 agcaacgatc tgccgttcat gaactggcgg ctcgcggtga gccttcgtga gctgctcgac
     2581 gccggcgtgc acgtgtttcg ctgtgcgggc gtgaacactt tgcacacgaa gctcatcgtc
     2641 gccgacgaac gcaagatcgt gttcggcagc acgaacctcg attactactc acccacctac
     2701 tgcgcggagc tcgacatcgc catcgagagc gcttctctcg gcgcggagct cgcaagcatc
     2761 atcgaagccg gcctcgcgct ttcggattcg gaggcggtgt tgccgggcac gggcacgagt
     2821 ctcgaacgtg aatgccgcgg gtggtcggtg tctcgcgtgt tcgaccttct gcttcacgac
     2881 atccaatgac gctgcatcct cccttcgagc caggccagtt gctcatcgcg gaagcgttgt
     2941 cggcggcgct cggaccgatt tcggttccgg agctcgagcg catccttctc agtgtgcgtc
     3001 tctcgcgttt cgcagccggg acgctcgtgc gcttcgatgc cgcagccgag ctccgcgtcg
     3061 tggttgccgg aatcgtggcc gtgcacgacc tcggtggagc cgacgaaacc aagtacttgc
     3121 cgggccagct cctgccttca gacgctcact cggcgccgta tcgagccttg cgcgatgtgc
     3181 tggtcgcgca cgttccaagc gaggccctcg agggctgggg cgcggaccga gtgctgcgcg
     3241 cgctcgcgcg gacgggcctt cgcgctcact cgaagccccc gctcctcgtc ggtatttcgg
     3301 ggaccgaccc ggagctggtc gccgagctac accgggctct tgcggcggcg ttggcccgat
     3361 ttggctcggt cagtgtcggc gtcgtggaac gctcgcgcgg cgcgctgccg agtcggctcg
     3421 agctctccgg cttgctcgcg cccgccgaat ttcaagtcgt ggcgctgccg tcgccggtgc
     3481 ggccctcgga cactcccgaa gtcgaccggt ggctgtacgt cgtcgatggc caacgcccgg
     3541 gtgagggttg tgcgatgccg cgcagggatg ccttcgagcg gcccggtgcg gtgcgactca
     3601 gcaccgcggt gccacgagct accgacgcgc tcgggcgcgc tccgtgcgtg ctcggcgagc
     3661 cagggttgat gcctcgtacg ctcgtcgtct gtcattcggg ctctcgccgt ctgcccagcg
     3721 cgacacgacg gcacctcgcg tttcacgaac ccgatgacca tcgtcacgtg cgtcgcggcg
     3781 acgtgggcca cgtcgagcgc cttgctcgcg agctcgctgg tgtgcccgtg ggcttcacct
     3841 tcggcgcggg cggggcgcgt ggcctcgctc acctcggcgt actctgcgct gcagaagagg
     3901 cgggcgtgcc cgtggattac atttcgggaa cgagcatcgg ggcggtgatt ggcgccctcg
     3961 ttgcaatggg tcacgacgcg gacgcccggc gcgaggcggc ggagcggctc gcgagcgcgc
     4021 ggccgttttg ggatttcacc ttacccacga ccgcgctctt gcgggggcgg cgcttcgaag
     4081 cgctcgcgga cgagttcttc ggc
//
LOCUS       DASTKS010000555         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1873435, whole genome shotgun sequence.
ACCESSION   DASTKS010000555 DASTKS010000000
VERSION     DASTKS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873435"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VFQ35_11440"
     CDS             complement(<1..1322)
                     /locus_tag="VFQ35_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET9931297.1"
                     /translation="MSTIVSKFRSRVAAEPDALALIFLQDGENDELRLSFAELDRRAW
                     SLAARLAEQGAFGERALLLYAPGVDYVVAFLACLYAGVVAVPAYPPDPARLQRSLPRL
                     LATVADSRCKFLLTTSQIASFGQAMLEFAPALREMRWLSTDDGNEASRDGFSTPDIRG
                     RDLAFLQYTSGSTGNPRGVMLTHDNLLHNLGLIASSLDVRKDTVGVSWLPPYHDMGLI
                     GAILCPLIHSGPLVLMSPLSFLQRPLRWLQAISKYRAEASGGPNFAYDLCVRRVSAEE
                     ASRLDLSAWRAAFCGAEPIRRDTLEAFGRHFAVSGFRSQAFYPCYGLAEATLIVSGAR
                     LGAGAQVFAADTQALMQNFVLRAEDPKSKTELVSSGNVLGEQSVIVVDPANGLALGEA
                     AVGEIWVAGPSVALGYWERSEESLATFRAYLADGRGPFLRTGDLGFLR"
     gene            complement(2029..>3040)
                     /locus_tag="VFQ35_11445"
     CDS             complement(2029..>3040)
                     /locus_tag="VFQ35_11445"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9931298.1"
                     /translation="LSSFLLHPDFNEVEVDVQRRLALARIASSADSSWHFRDIVLAKI
                     PGFERSSPKENAVRLLELKPPLLRRIHACTVRPDAAELVVVGPVSVEAVTAQAERAFD
                     SWETAPPDATKTCEWPQTVASAPGETVQRLARTELQLVYGDNDPFITIAADGPSLESE
                     DFLPFILLADVLEKRAQGSAKRLRHTGGTYGIWASVTEEAPGRSVLILRGQLDADVAQ
                     DALRALLQDIGVLSRVLDEGELESRKRAWRNELVTSMSSNMSLANVLLSGMARKREVT
                     TLPDMPNEIMRIDAARCRDVAERWLASAHPSILVVTGKPRTFLRGLGVDAHVELKYFS
                     AR"
BASE COUNT          608 a          985 c          963 g          484 t
ORIGIN      
        1 tcccgcaaga agccgagatc gccagtgcgt agaaatgggc cgcgcccgtc ggccaggtac
       61 gcgcggaacg tagccagcga ctcttccgag cgctcccagt aaccgagcgc gacgctcggc
      121 ccggccaccc aaatctcgcc gacggctgcc tcgcccagcg cgagtccatt ggcggggtcg
      181 acgacgatga cgctctgctc tccgaggacg tttccagagc tcacgagctc ggtcttcgac
      241 ttcgggtctt cggcgcgcaa cacgaagttt tgcatgagcg cctgcgtgtc ggcggcaaag
      301 acctgcgcgc cggcgccgag gcgagcaccc gagacgatga gcgtcgcctc cgccaacccg
      361 tagcagggat aaaacgcctg cgagcgaaac ccgctcaccg caaagtgcct gccgaacgcc
      421 tcgagcgtgt cccgccggat cggctcggcg ccgcagaaag ccgcgcgcca cgccgaaaga
      481 tccaggcggc tcgcctcttc cgcgctcacg cggcgtacgc agaggtcgta cgcgaaattc
      541 ggaccgcccg aggcttctgc gcgatacttc gagatcgctt ggagccagcg cagcggtcgc
      601 tgcaaaaacg acaacggcga catcagcacc agcggcccgc tgtgaatcaa agggcacagg
      661 atcgcgccga tgagccccat gtcgtgatac ggcggcaacc aactgacccc caccgtgtct
      721 ttccggacgt cgagcgaaga cgcaatcagg ccgaggttgt gcagcaggtt gtcgtgcgtg
      781 agcatcacgc cacgcgggtt cccggtagaa cccgaggtgt actgaaggaa ggcgaggtcg
      841 cgcccacgaa tgtcgggggt gctgaagccg tctcgcgacg cctcgttacc gtcgtcggtg
      901 ctgagccagc gcatctctcg cagggcgggc gcgaactcga gcattgcctg gccgaacgaa
      961 gcgatttggc tcgtggtcag caggaacttg cagcgtgaat cggccaccgt cgcgagaagg
     1021 cgcggtaggg accgctgcag acgcgccggg tccggcgggt acgctggcac cgccaccacg
     1081 cccgcgtaaa gacatgccag aaaggcgacc acgtagtcga caccgggcgc gtagagcagc
     1141 agggcccgtt caccgaaagc gccttgctcg gcgagcctcg cggcgaggct ccacgcacgc
     1201 cgatcgagct cggcgaacga gagcctgagc tcgtcgtttt ctccatcttg caggaagatc
     1261 agcgccagag cgtcgggctc cgcagcaact ctcgagcgaa acttcgaaac gatcgtgctc
     1321 atgaggatcc gtgagtccgt tcagtcaccg tgtgcacgcg gttgaggcag cagtgctgcg
     1381 ctcggcggaa cggaacgatg agcctagacc agaagaatgc gcgaaactag tcggaagcat
     1441 tagtcacccc tccggatgat ggccgcagcc aagagctcca cgaagctcga cttcatctta
     1501 cgaccgagcg gcgagcctca tgagcccgtc gcacgcgtcg gcgcgcgctc gccgagcact
     1561 gcctcgcgat aggcaattct ttgcaaaatc gcgcaaaaca ccgcagtttc acgggtcctt
     1621 cgagacaacc tactcgaagc acacacctcg agcccatgtg agagttttct gcggtgaacg
     1681 acctcacagg ccaccgaaac gaaacaacaa atctcctaaa gtgcgcgcac tcatggcgct
     1741 cgagtaggtg ctcgaccgaa cgcgcaactg cgctcgctgc gcgccccaaa acgagcgagt
     1801 tggagcaccc tgccggccga tccggaacgc tccaaggcga gacgctcgag cgaatagaaa
     1861 ggggtattac ctacggacga ggcacacgtt cgccgaagcg ctgcgcgctc tcgagcgcgt
     1921 catgccgccg ggtgacaaac cacgctaatc gaggccgaaa tgcgcgtaaa agaggccacg
     1981 acccccggtg gatatgtgcg acctcagtcg cgacgcgcgc gttcgaggct accgcgcgct
     2041 gaaatacttg agctcgacgt gggcgtcgac gcccagccct cggaggaagg ttcggggctt
     2101 gccggtaaca acgaggatgg atgggtgtgc ggacgcgagc cagcgttcgg ccacgtcccg
     2161 gcaacgcgcc gcatcgatgc gcatgatttc gttcggcatg tcaggcagcg tcgtgacttc
     2221 ccttttccgc gccattccgg acaggagcac gttggcgagg ctcatgttgg aggacatgga
     2281 ggtgacgagc tcgttgcgcc atgcgcgttt gcggctctcg agctcgcctt cgtcgagcac
     2341 tcgagagagc accccgatat cttgcaacag cgcacgcaaa gcgtcctgcg cgacgtccgc
     2401 gtcgagttgt ccgcgcagaa tgagcacgct tcgccccggc gcctcctctg ttacgctcgc
     2461 ccaaatgccg tacgttcccc cggtgtggcg tagccgcttg gcggagcctt gggctcgctt
     2521 ttccaagacg tccgcgagca aaatgaaggg caaaaagtct tctgactcga gtgaaggccc
     2581 gtccgccgcg atggtgatga aagggtcgtt gtctccgtag accaactgaa gctcggtgcg
     2641 cgccaagcgc tgcaccgtct ctcctggcgc cgacgcgacc gtttgcggcc actcacacgt
     2701 cttggtggcg tcgggcggag ccgtctccca tgaatcgaag gcgcgctcgg cctgagcagt
     2761 gacggcttcg accgagaccg ggcccacgac gaccagctct gcggcatcgg gacgcacggt
     2821 gcacgcatga atcctccgca gaagcggcgg cttcaattcg agcaaccgca ccgcgttttc
     2881 cttgggtgag ctgcgctcga agccagggat cttcgcgagg acgatgtctc gaaaatgcca
     2941 actcgagtcg gcggaagatg cgatgcgagc cagcgcgagc ctgcgctgaa cgtcgacttc
     3001 gacttcgttg aaatcagggt gtagcaggaa cgacgagagc
//
LOCUS       DASTKS010000556         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_756365, whole genome shotgun sequence.
ACCESSION   DASTKS010000556 DASTKS010000000
VERSION     DASTKS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756365"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFQ35_11450"
     CDS             complement(<1..438)
                     /locus_tag="VFQ35_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9931299.1"
                     /translation="MKAPPRVDRRVQRTRRALSEALIGLILERGWDDVSVQDVCDRAN
                     VGRSTFYTHFADKEELLVGGLDDLRMGLRQLAPVEAGARPLGFLRGIIEHADEHRRLF
                     RAVIGKRSGHAVQQRFRQMLIDLIQEDLAAVAPAGPHLDVTARY"
     gene            550..1287
                     /locus_tag="VFQ35_11455"
     CDS             550..1287
                     /locus_tag="VFQ35_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931300.1"
                     /translation="MSSNGHGVHVVELQAVTKIYPGADGGFTALGGVDLALSAGELTA
                     VVGKSGSGKSTLMNLIAGLDRPSRGAVLVAGVPLHSLDESTLSAWRGRNVGVVFQFFQ
                     LLPTLTIAENVMLPMDFCGTYPPAQARTRARELLDRVGIADQANKLPSALSGGQQQRA
                     AIARALANDPPLVVADEPTGNLDSRTASAVMLLLSELASMGKTVVVVTHERDVVSRFD
                     RVVTLADGRVAEQTLSKGRAAAVAVPC"
     gene            1281..>3511
                     /locus_tag="VFQ35_11460"
     CDS             1281..>3511
                     /locus_tag="VFQ35_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9931301.1"
                     /translation="MLSPRWRKLFRDLSMERGRALLMVTAIALSIGGIGAVLGAYSIM
                     TREMPRNYLGTRPASAALELRQVDRALVEKVRRYPGVAEAEAGDIVLSRAKVGEDWIP
                     LLLFVVDEFGAMRLNTFTRTSGAWPPPEGTMLIERSAVPMLKATEGDSVLVKTPHGRT
                     RSVKITGVVHDPGLAPAWQEREGYGYVTPATLASLGEPPSLGELRVELSDPGVDVRGI
                     ETKVNELARWLGTQGHPVSRVRVPPPRRHPHQSQMMGVLFLMLAFSGMALVLSAVLVA
                     TSVSALLARQVREIGVMKTLGARTGQLGLLYAALIVVLGSVAVALALPLGVVGARGLA
                     RMSAGMLNLTIATYAIPWWVFAIQIAAGVLVPLAAASAPIVRGCRVTVREALDRHGAS
                     QPALRSRGVALLHKLTWTSRVTSLALRNAFRQRTRTMLTLVLLAAGGAMFMTALDISR
                     GWARIVDRVYENRSYDVEIRLNSPAQLTEPLQGVPGVRKVEAWAYSHTALWKPNRVDV
                     VRTYPDGSHGSLALIGPPPTTDLVHFPLLAGRWLLPQDTDAVVINHMVLGQAPGTKVG
                     DELTFSLEGRPTTWRVVGIVEEVGSPGAAYVSREAFERLSGTPGKARMLRVATTATSP
                     NERTAVIRAIERRLEQERADVEAVIPLSVLRTAMGDHIVVLIRMLLAMAALMVTVGML
                     GLASTMGTNVLERTREIGVMKTIGATPNQVARLVVSEALFVGVVSWLAAVVLAVPLTA
                     LVG"
BASE COUNT          567 a         1159 c         1177 g          608 t
ORIGIN      
        1 gtagcgcgcg gtcacgtcga ggtgcggccc ggcgggggca accgcggcca agtcttcttg
       61 gatcaagtcg atcagcattt gacggaagcg ctgttgaacg gcgtgaccac tccgcttacc
      121 gatgacggcg cgaaacaggc gccggtgctc gtcggcgtgc tcgatgatac cgcgcaggaa
      181 gccgagcggc cgcgcgccgg cctccacggg cgcgagctgg cgcagtccca tgcgcaagtc
      241 gtcgagacca ccaacgagca gctcttcctt gtcggcgaag tgcgtgtaga aggtggagcg
      301 accgacgttg gcgcgatcgc acacgtcttg gacgctgacg tcatcccaac cccgttcgag
      361 aatcagcccg atgagggcct cgctcagggc tcggcgcgtt cgctggactc ttcgatcgac
      421 gcgtggcgga gccttcacgc ccaaggcagc ctcacttcct ggacagatcg ctctgcactg
      481 ttcgaaactg cgcaaaagcg ggctcgctgt cggttgcggg tgagaatcga cgtctgcatg
      541 ctgcacacta tgtctagtaa cggtcacggc gtccacgtcg tggagctcca ggcggtcacc
      601 aagatttacc cgggcgcaga cggaggtttc accgcactcg gcggggtcga tctcgcgctc
      661 tcggcggggg aactcaccgc ggtcgtgggc aaatcgggga gcggcaagtc cacgttgatg
      721 aacctcatcg cggggctcga tcggccctcg cgcggagccg tgctcgtcgc cggcgtaccg
      781 ctgcattcgc tcgacgaaag cacgctctcg gcttggcgcg gtcgcaacgt aggtgtcgtg
      841 ttccagttct tccagctgct gcctaccctg accatcgcgg aaaacgtgat gttgcccatg
      901 gatttctgcg gcacgtatcc gcccgcgcaa gcgcgcacgc gtgcccgcga gctgctcgac
      961 cgggttggga ttgccgatca ggcaaacaag ctcccgagcg ctctgtccgg cggtcagcag
     1021 cagcgcgccg ccatcgctcg cgcgctcgcg aacgatccgc cgctcgtagt agcggacgag
     1081 cccaccggca acctcgactc gcgcaccgcg agcgcggtga tgcttctctt gagcgagctc
     1141 gcctcgatgg gaaagaccgt cgtggtggtc acccacgagc gcgacgtggt gagccgtttc
     1201 gaccgtgtag taaccctcgc cgacggtcgc gtcgcagagc aaacactgag caagggtcga
     1261 gcagcggcgg tggcggtgcc atgctgagcc cgcgctggcg caagctcttt cgtgacctct
     1321 cgatggagcg gggtcgcgct ctcttgatgg tgacggcgat cgctctcagc atcggaggca
     1381 tcggggcagt gctcggcgcg tattccatca tgacgcggga aatgccgcgc aactacctcg
     1441 gtacgcgccc tgcctcggcc gcgctcgagc tgcgccaagt cgatcgagcg ttggtcgaga
     1501 aggtgcggcg ctaccccggg gttgcggagg cggaagccgg ggacatcgtg ctctctcgag
     1561 ccaaggttgg cgaggactgg atcccgctat tgctcttcgt ggtcgacgaa ttcggtgcga
     1621 tgcggctcaa caccttcact cgaacgagcg gcgcgtggcc tccgccagaa ggcacgatgc
     1681 tcatcgaacg ctctgcggtg ccgatgctga aggcgaccga gggcgacagc gtgctcgtca
     1741 agacgccgca cggccgcacg cgttcggtga agataacggg cgtggtgcac gatccggggc
     1801 tcgctccggc atggcaggag cgcgaaggct acggttacgt cacgccggcg acgttggctt
     1861 cgctcggaga acccccgtcg ctcggcgagc tccgggtaga gctgagcgac cccggcgtcg
     1921 acgtgcgcgg catcgagacg aaggtcaacg agctcgcgcg ctggctcgga acccagggcc
     1981 acccggtctc acgagtacgg gtgccaccgc cgcgcaggca cccgcaccaa agtcagatga
     2041 tgggcgtgct gttcctgatg ctcgccttca gcggcatggc gctggttttg agcgccgttc
     2101 tcgtggccac gtcggtctcg gcgctgctcg cgcgccaggt ccgggagatt ggcgtgatga
     2161 agacgctcgg tgcgcgcacc gggcagctcg gtttgctcta cgcggcgctg attgtcgtcc
     2221 tcggttcggt cgcggtcgcg ctcgcgctgc cgcttggagt ggtcggcgcg cgcggcctcg
     2281 cgcggatgtc ggccggaatg ctgaacctca ccatcgcgac ctacgccatc ccttggtggg
     2341 tcttcgcaat ccaaatcgcg gccggagttc tggtgccact cgcggccgcg agcgcgccca
     2401 tcgttcgagg atgtcgcgtt accgtgcgtg aggcgctcga ccgccacggc gcgagccaac
     2461 cagcgttgcg aagtcgcgga gtggcgctgc tccacaagct cacctggact agccgcgtca
     2521 cttcgctcgc gctccgcaac gccttccgac aacgcacgcg gaccatgctg accctcgtgc
     2581 tgctcgccgc gggcggcgcg atgttcatga ccgcgctcga catctcgcgc ggctgggcgc
     2641 gcatcgtcga tcgcgtttac gaaaaccgca gctacgacgt cgagatccgc ttgaactctc
     2701 cggcgcaact cacggagccg ctgcaaggcg tccccggcgt gcgaaaagtc gaggcctggg
     2761 cctacagcca caccgcgctt tggaagccga atcgcgtcga tgtcgtgcgc acctatccgg
     2821 acggcagcca cggcagcttg gcgctgattg ggccgccccc aacgaccgat ctcgttcatt
     2881 tccctttgct cgccgggcgc tggcttttgc cgcaagacac cgacgcggtc gtgatcaacc
     2941 acatggtctt gggccaggca ccgggcacga aggtcggaga cgagctcacc ttctctctcg
     3001 aagggcgtcc caccacgtgg cgtgtcgtgg gaatcgtcga ggaagtcggc tcaccgggcg
     3061 cggcgtacgt ttcgcgagaa gcgttcgaac ggctcagcgg cactccgggc aaggcgcgca
     3121 tgctgcgcgt cgcaaccacc gcgacttctc cgaacgagcg caccgcagtc attcgagcga
     3181 tcgagcgccg tctggagcaa gagcgggccg acgtcgaggc ggtgatccct ctcagcgtac
     3241 tgcgcaccgc catgggcgat cacatcgtcg tgctgatccg catgctgctg gcgatggcgg
     3301 cgctgatggt aaccgtcgga atgcttggcc ttgcatccac gatgggcacc aacgtgctcg
     3361 agcgcacccg cgaaatcggc gtaatgaaga ccatcggcgc cacgcccaac caagtggcgc
     3421 ggctcgtcgt cagcgaggcg ctcttcgtcg gtgtggtgag ctggctagca gcggtcgtgc
     3481 tcgcggtgcc gctcaccgct ctcgtaggaa a
//
LOCUS       DASTKS010000557         5777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1873928, whole genome shotgun sequence.
ACCESSION   DASTKS010000557 DASTKS010000000
VERSION     DASTKS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873928"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFQ35_11465"
     CDS             <1..628
                     /locus_tag="VFQ35_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9931302.1"
                     /translation="PLISDDALLDVAREVFLERGFQATTEEVAERAGVSEGTVFRRFK
                     SKDALFRAAMRFSPDAVPAFIDSFVSEVGQGDLRERLLSFATRLLELGRTAMPIMMMS
                     WSNPRECEFDKLSQRTEGYKKTFQSVVRFFSRELAARKLRAEKAELLARIFMGSLHHY
                     CMAELFLGSSQISPSREDFTQGLVDVLLSAAGHALPQAPPRRGSRRSE"
     gene            657..2114
                     /locus_tag="VFQ35_11470"
     CDS             657..2114
                     /locus_tag="VFQ35_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9931303.1"
                     /translation="MKNKASIAGAALALIVALVAPRSAHATQPLQEFIDRASSQSFDA
                     RESNALVSQREAEADAALGGLLPTASARGVYTRNQYEVAIPAQSTMGASTKPIVIQRH
                     NQLDGFLQLDVPLVNVSNYHRYKSGQALAQSTKEQRAAISLDVGRSVTRAYFNFLGAN
                     ALVRSAELSLKATEDNLRVVEARRDAGAATELDRERARANVERARQDVADAKLMVDLA
                     ARQLETLSGLTPTPAESFPVDDLHDEAPVATWLSQAEKTPLAKSAKSAQLAAQEARKA
                     ADTALYPTLAGSAQEHFTNAGGFAGRNAFYTLQLVAQVRFDYALIAQSRAQAFAADAS
                     GVRSERTVRSLQDATYEAYRRVETGIVKSRSARAQAAAAARASSLSSERYAAGVATQL
                     DVTQAQRDAFLADAARIQADADLSAARAQLRLSVGEAPPPTNAQNSVPLESVPAEPGG
                     SSAVSSPTPPPAGAAPAPPPSPANPTPAPNPTRKP"
     gene            2114..3205
                     /locus_tag="VFQ35_11475"
     CDS             2114..3205
                     /locus_tag="VFQ35_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9931304.1"
                     /translation="MSSPRASALRFLPLASLLFGFASACNKTATAEGDAGASPLDKPI
                     EVKTAPVSVLEVPTTLRLSGSLKGNQETDLAANAAGRVLATQVERGATIAPGQVLARL
                     DVRAAALSAADARAQAASIQAQQQSAAQECARYEQLKQKGAVSDLEYDRVQAQCRTLP
                     LNVQAATLRAQLAAQNVGDGVIRAPFAGVVTERYVEVGQYVRQDSRVVTIVSLDPLRL
                     ELAVPESEVGRVKVGATVSFSVAAFPSQKFTGEIRFVSGALRSTTRDLVAEAIVKNPD
                     RLLLPGMFADVELTTGTQKLPGVPKAALLNRDGQAHAFFVVDGRLEERILSLGPTTNE
                     TASVLKGAAPNQVVALGPFEQLSNGQRVR"
     gene            3224..>5777
                     /locus_tag="VFQ35_11480"
     CDS             3224..>5777
                     /locus_tag="VFQ35_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9931305.1"
                     /translation="MQWLAQVCVKRPVFAGVLMLVIVVLGLVGYTRLGLDQFPNIDLP
                     FVLVTTRLDGAAPEEVETDISDKIEGAVNTIEGIDELRSTSSEGFSQVAIAFKLDKNG
                     DVGAQDVRDKINTVLRDLPKGIDAPVVTKVDPQSAPIMLIALRSNQGIRELTEIADKR
                     VRRQIESISGVGQVSIIGGRARQIHVWLNPVRLRSYGLSSVDVERAIASQNLSTPGGS
                     LDTGPQSITLRIAGRVENVQGIERIVVRQQGDHPIRVSDVARVEDGEEQIASVASYDG
                     ERTVVLAIRKQSGTNTVQVVDQVKKRLLEVKRSLPRGIDLQIVRDNSQSIRTGVDSVK
                     EHLFVGAVLAALVVLLFLGNARSTLIAAIAIPISIIGTFALMWIEGFSLNMLTLLALA
                     LAVGIVIDDAIVVLENIVRYVTEKGVKPFPAAILATREIGLAVLATTLSLMAVFVPVA
                     FVGGIPGRFLKNFGYTMAFAVGVSLFVSFSLTPTMSARLLRSDIHSEDSKLGHLVNIF
                     YRPIERVYMAILTWAMKRRWVVVLLSAASLGSCGPLMKHVPTGFVPVDDQAQFEVSMR
                     APEGTSAEETALIAERVAQEVRHLTGVEHTVVTVAGGDQQTENLASVYVALSDPRERK
                     ISQLELMDKVRKEILTKLPPNLRVSVAEVAMINAGASSAAVQYVLVGPDLHKLEDLAG
                     KMGPLIKKHPAVVDFDTNLILGKPELRVDIDRARASDLGVSVADIAETLRMQVAGVKI
                     SSYAEQGEEYDVRLRADQDYRRDASALALITVPSTKYGSVPLTSVVRTSSGSGPSQVN
                     RLGRQRQVTFMANVAPGRGESEVVKAMQDAFKSVNAPPEYRLVPTGRSKSSAE"
BASE COUNT         1007 a         1958 c         1864 g          948 t
ORIGIN      
        1 gccattgatt tcggatgatg cgctgctcga tgtggcccgg gaggtgttcc tcgaacgcgg
       61 ttttcaagcc acgacggaag aggtggcgga gcgcgcaggt gtttcggaag ggacggtgtt
      121 caggcgcttc aagagcaagg acgcgctgtt ccgcgccgcg atgcgcttca gcccagacgc
      181 cgtgcccgcg ttcatcgaca gcttcgtgtc agaggtggga cagggtgatt tgcgagagcg
      241 cttgctgtcg ttcgcgacgc gcttactcga gctcgggcgc acggccatgc ccatcatgat
      301 gatgtcgtgg tcgaacccgc gcgagtgtga gttcgataag ctctcgcagc gcaccgaagg
      361 gtacaagaag acgttccaga gcgtggttcg cttcttctcg agagagcttg ctgcgcgcaa
      421 gctgcgcgcc gaaaaggccg agctcctcgc caggattttc atgggcagcc tgcatcacta
      481 ctgcatggct gagctcttcc tcggctcctc gcagatctca ccttctcgcg aggacttcac
      541 ccaggggctc gtcgacgtgc tgctctccgc ggccggccac gcgcttccgc aagcaccccc
      601 acggcgagga agccgacgca gcgaataggc cctcaacggc ttggaatgta cgcatcatga
      661 agaataaagc atcgatcgcg ggcgccgcct tggcgctcat cgtggcgctc gtagcgccgc
      721 gctcggctca cgccacccaa ccccttcaag agttcatcga tcgcgcaagc tcgcagagct
      781 tcgacgctcg ggagagcaac gcgctcgtat cgcagcgcga ggccgaggcg gacgcggcgc
      841 tcggcggctt gttgccgacg gcttcggccc gcggtgtgta cacgcgcaac cagtatgaag
      901 ttgcgatccc agcccagtcc acgatggggg catcgacgaa gccgatcgtc attcaacgcc
      961 acaatcagct cgacggcttt ctgcagctag acgtgccact cgtgaacgtc tcgaactacc
     1021 accggtacaa gtcgggccag gcgctagcgc aatccaccaa agagcagcgc gccgccattt
     1081 cgctcgacgt ggggcgtagc gtgacgcgcg cctacttcaa ctttctggga gcgaacgcgc
     1141 tcgtgcgctc cgcggagctc agcttgaagg cgaccgagga caacttgcgt gtcgtcgaag
     1201 cgcgacgtga cgcgggagcc gccaccgagc tcgatcgcga acgcgcgcgc gccaacgtgg
     1261 aacgcgcgcg ccaagacgtg gccgacgcca aactcatggt ggatctcgcg gcgcggcagc
     1321 tcgaaacgtt gagcgggctc accccgactc ccgccgagag cttccccgta gacgatctcc
     1381 acgacgaagc tcccgtcgcg acctggctgt ctcaggcgga gaagacgccg ctcgccaaat
     1441 cggcgaagag cgcgcagctc gcggcgcaag aggcacgcaa agccgctgac accgcgcttt
     1501 acccgacgct cgccggctcc gcgcaggagc acttcacgaa cgcgggcggc ttcgcggggc
     1561 gcaatgcgtt ctacaccttg cagctcgtcg cgcaggtccg cttcgactac gcgctgatcg
     1621 cgcagagtcg cgcgcaggcg ttcgcggccg atgcgagcgg tgtgcgcagc gagcgtacgg
     1681 tgcgaagcct tcaagacgcg acttacgagg cgtaccgccg cgtcgagact ggcatcgtga
     1741 agagccgctc ggcgcgcgcc caagctgcgg ccgccgcgcg cgcttcgtcg ttgtcgtccg
     1801 agcgctatgc ggccggcgtc gcgactcagc tcgacgtgac gcaggcgcag cgcgacgcgt
     1861 ttttggcgga tgccgcccgc atccaagccg acgcggatct ctccgccgcg cgcgcgcagc
     1921 ttcgcctgtc ggtcggtgag gctccgccgc caaccaacgc gcagaattcg gttccgctcg
     1981 agagcgttcc cgctgaaccc ggagggagtt cggccgtttc gtctccgacg cctccgccag
     2041 ccggcgcggc ccctgcccct cccccaagtc ccgccaatcc cacacccgct cccaacccga
     2101 caaggaagcc gtgatgtcca gcccgcgcgc gagtgcgctt cgtttcctac ccttggcgag
     2161 cctgcttttt ggcttcgcca gcgcttgcaa caagaccgcg accgcggaag gcgacgccgg
     2221 cgcgtccccg ctcgacaaac ccatcgaggt gaagacggcg ccggtctccg tactcgaggt
     2281 tccgaccacg ctccgcctct cgggcagctt gaaggggaac caagagacgg atctcgccgc
     2341 aaacgccgcg ggccgagtgc tcgcgacaca ggtggagcgc ggagcgacca tcgcgcccgg
     2401 acaggttttg gctcgcctgg atgtgcgcgc cgcagctctt tcagccgcag acgcccgagc
     2461 ccaggccgcc agcatccagg ctcagcagca gtcggcggcg caagagtgcg ctcgctacga
     2521 gcagctgaag caaaagggtg cggtgagcga cctcgagtac gatcgcgtac aggcgcagtg
     2581 ccgtacgttg ccgctcaatg tgcaggcggc gaccttgcgc gctcagctcg ccgcgcagaa
     2641 cgtgggcgac ggagtcatcc gggcaccgtt cgctggcgtc gttacggagc gttacgtcga
     2701 ggtggggcag tacgttcggc aggactcgcg agtcgtcacg atcgtttcgc tcgacccact
     2761 gcgtctcgag ctcgcggtgc cagagtccga ggtcgggcgc gtgaaggtcg gcgcgacggt
     2821 gagcttttcg gtcgcggcgt ttccgagcca gaagttcact ggagaaatcc gtttcgtgtc
     2881 cggtgcgctg cgctccacga cccgtgacct cgtcgcggag gcgatcgtga agaaccccga
     2941 ccggctgctc cttccgggga tgttcgccga cgtcgagctc acgaccggta cgcagaagct
     3001 gcccggcgtt ccgaaggcag cgttgctgaa ccgcgacggc caagcgcacg ccttcttcgt
     3061 ggtggatggc cgcctcgagg agcgaatctt gtcgctcggc cccacgacga atgaaacggc
     3121 gagcgtgctg aagggcgccg ctccgaacca agtcgtcgcg ctcggcccgt tcgagcagct
     3181 ctccaacggt cagcgcgtgc gctgaactca aaggcaacga cccatgcaat ggcttgctca
     3241 ggtctgcgtg aagcgcccgg tcttcgccgg cgtgttgatg ctggtgatcg tggtgctcgg
     3301 gctcgtggga tatacgcgcc tcggcctcga tcagttcccg aacatcgacc tgccgttcgt
     3361 gctcgtcacg acccgcctcg acggcgccgc gccggaggaa gtcgagaccg acatcagcga
     3421 caagattgaa ggcgcggtca acacgatcga aggcatcgac gagctgcgct cgacctcgag
     3481 tgagggcttt tcgcaggtcg cgatcgcctt caagctcgac aagaacggcg acgtcggcgc
     3541 gcaagacgtg cgcgacaaga tcaacaccgt gctgcgcgac ttgcccaagg gcatcgacgc
     3601 gccggtcgtc accaaggtcg acccgcagtc ggcgccgatc atgctcatcg cgctacgctc
     3661 caatcagggc atccgcgagc tcaccgaaat cgccgacaag cgcgtgcgca ggcagatcga
     3721 gagcatttcc ggggtcggtc aggtcagcat catcggcgga agggcccgcc agattcacgt
     3781 ctggctcaac ccggtgcgcc tcaggagcta cggcctcagc tccgtggacg tcgagcgcgc
     3841 catcgcttcg cagaacctgt ccacgccggg tggaagcctc gacaccggac cgcagtcgat
     3901 cacgctccgc attgccgggc gcgtggagaa cgtgcagggc atcgagcgca tcgtcgtgcg
     3961 gcagcagggc gatcacccca tccgcgtgag cgacgtcgcc cgggtcgagg acggcgagga
     4021 gcaaatcgcg agcgtggcga gctacgacgg cgagcgcacg gtggtcctcg cgatccgcaa
     4081 gcagtcggga acgaacacgg ttcaggtggt agaccaggtc aagaagcgcc tgctcgaggt
     4141 gaagcgctca ttgccgcgcg gcatcgatct ccagatcgtg cgcgacaatt cgcagtccat
     4201 ccgcaccggc gtcgattcgg tgaaagagca cttgttcgtc ggcgcggtgc tcgcggccct
     4261 ggtcgttctg ctcttcctcg gcaacgctcg cagcaccttg atcgcggcca tcgcgatccc
     4321 gatttcgatc atcggcacct tcgccctgat gtggatcgaa ggcttcagct tgaacatgct
     4381 cacgctgctc gcgctcgctc tggcggtggg catcgtgatc gacgacgcca tcgtggtgct
     4441 cgaaaacatc gtgcgctacg tgaccgaaaa gggcgtgaag cccttccccg ccgcgatcct
     4501 cgcgacgcgg gaaatcggcc tcgcggtgct cgcgacgacg ttgtcgctca tggccgtgtt
     4561 cgtgccggtc gcgttcgtgg gcggcatccc gggccgcttc ttgaagaact tcggctacac
     4621 catggccttc gcggtgggtg tgtcgctgtt cgtcagcttc tcactgacgc cgacgatgag
     4681 cgcgcgcctc ttgcgctccg acattcactc ggaggacagc aagctcggac acctggtgaa
     4741 catcttctac cggcccatcg agcgcgtcta catggccatc ctcacctggg ccatgaagcg
     4801 acgctgggtc gtcgtgctgc tctcggccgc gtcgctcggc tcgtgcggcc cgttgatgaa
     4861 gcacgtcccc accggcttcg tgcccgtcga cgatcaggcg cagttcgagg tcagcatgcg
     4921 cgcgcccgaa ggcaccagcg ccgaagaaac cgcgctcatc gccgagcgcg tcgcgcaaga
     4981 ggtacgtcac ctgaccggcg tcgagcacac cgtcgtgacg gtcgcgggcg gcgatcaaca
     5041 gaccgagaac ctcgcgtcgg tgtacgtggc cctgagcgat ccccgcgagc gcaagatctc
     5101 gcagctcgag ctgatggaca aggtccgcaa agagatcttg acgaagctcc cgccaaacct
     5161 gcgcgtcagc gtggccgagg tcgccatgat caacgccggc gcctcgagcg cggcggtgca
     5221 gtacgtgctc gtgggcccgg acctgcacaa gctcgaggac ctcgccggca agatgggtcc
     5281 gctcatcaaa aagcaccccg cggtcgtgga cttcgacacc aacctcatcc tcggaaagcc
     5341 cgaactccgc gtcgacatcg accgcgcccg cgcctcggat ctcggcgtga gcgtcgccga
     5401 catcgccgag acgctgcgca tgcaagtcgc cggcgtgaag atctcgagct acgcggagca
     5461 aggcgaagag tacgacgtgc gtctgcgcgc cgaccaagac taccgccgtg acgcctccgc
     5521 gctcgcgctc atcacggtgc cttcgaccaa gtatggcagc gtcccgctca cctccgtggt
     5581 tcgcaccagc agcggcagcg gtccctcgca ggtgaaccga ctcggtcgtc aacgccaggt
     5641 cacgttcatg gcgaacgtcg cgcccggccg cggcgagagc gaggtcgtga aggccatgca
     5701 agacgccttc aagagcgtga acgctccgcc cgagtaccgc ctggttccca ctggtcgctc
     5761 gaagtcgtct gcagagc
//
LOCUS       DASTKS010000558         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1428390, whole genome shotgun sequence.
ACCESSION   DASTKS010000558 DASTKS010000000
VERSION     DASTKS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428390"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VFQ35_11485"
     CDS             <1..1278
                     /locus_tag="VFQ35_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9931306.1"
                     /translation="DRSRSIGTQIAAIQKGFLPYDQLASNPQQLSADYERLRLSHELS
                     REIALERDLPSLLTKILTTIFKFVRADRGVIFLKGRDGEIVPSASLRRDGTDAPIPVS
                     STIMNHVVKERATVLTHDAAMDFAASKGKSMILNRISSAIVAPLLHNDEILGVLWLDS
                     ETLAQFQPKDMEIVTAIAAQAAMFIEISILGKKIEQEIVNRERFSRLLSPNVAERVLS
                     GELEVKKGGQLVRECTVFNSDIRGFTAMSEGSQPEVLVEMLNEYFELMVETVFKYEGT
                     LDKFMGDGIMALWGAPVVHPDDPLRCVSCALEQMEVLGRFNRRRLENNQPLLAIGIGV
                     HTGPLVAGYIGSSRALSYTVIGDTANTSARLCGVAAPGQILVSEATLARLGGRFEVEE
                     LPPAQLKGKEKPFRVFNVTRMRPSVQVAQAIGT"
     gene            1450..>2214
                     /locus_tag="VFQ35_11490"
     CDS             1450..>2214
                     /locus_tag="VFQ35_11490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HET9931307.1"
                     /translation="MKFVHAADLHIDSPLRGLERYQGAPVEIIRGATRRAVEHLVDLC
                     LEEGVKLLVLCGDLYDGDWKDYSTGLFFCQQMARLREADVVVAWIRGNHDAASRLTQH
                     LRPTDNVHELSHARPITLEREIDGVSVALHGQGFSKAKVTEDLTDRYPAPVSGAFNLG
                     LLHTALDGREGHDAYAPCRVESLINKGYDYWALGHVHQREQVNREPWIIFPGNLQGRH
                     ARETGKKGATLVTVSGGRVTEVEARALDEVRWAHVVY"
BASE COUNT          418 a          729 c          685 g          382 t
ORIGIN      
        1 gaccgctcgc gctcgatcgg cacgcaaatc gcggcgatcc aaaaaggctt tttgccttac
       61 gaccagctcg cgagcaatcc gcagcagctc tcggcggact acgagcgact gcgtctctcc
      121 catgagcttt cgcgcgaaat cgcgttggag cgcgacttgc cgtcgctgct caccaagatc
      181 ctcacgacca ttttcaagtt cgtgcgcgcc gaccgcggcg tcatcttttt gaagggacgc
      241 gacggcgaaa tcgtcccgag cgcgagcttg cgaagagacg gcaccgacgc gccgatcccg
      301 gtgtcgtcga ccatcatgaa ccacgtcgtg aaagaacgag cgaccgtgct cactcacgac
      361 gcggcgatgg acttcgcggc gtcgaagggc aagagcatga ttctgaatcg aatcagctcc
      421 gccatcgtcg cgcccctcct ccacaacgac gaaatcctcg gggtgctctg gctcgacagc
      481 gagacgctcg cgcagttcca gcccaaagac atggagatcg tgacggccat cgcggcgcaa
      541 gcagcgatgt tcatcgagat cagcattttg ggtaagaaga tcgagcaaga gatcgtcaat
      601 cgcgagcgct tcagtcgctt gctctcgccc aacgtcgccg agcgtgtgct ctcgggtgag
      661 ctcgaggtga agaagggcgg ccagctcgtg cgcgagtgca ccgtcttcaa ctcggacatc
      721 cgtggcttca ccgcgatgag cgaaggcagc cagccagagg tgctggtcga aatgctcaac
      781 gagtatttcg agctcatggt cgagacggtg ttcaagtacg aaggcacgct cgataagttc
      841 atgggcgacg gcatcatggc tttgtgggga gcgccggtcg tccaccccga cgacccactg
      901 cgctgcgtct cgtgcgcact cgagcagatg gaagtcttgg ggcgcttcaa ccgtcggcgc
      961 ctggagaaca accagccgct gctcgcgatc ggcatcggcg tgcacaccgg ccccctcgtc
     1021 gccggctaca tcggcagctc gcgcgcgttg agctacaccg tcatcggcga caccgcgaac
     1081 acgagcgcgc ggctctgcgg cgtcgccgcc ccgggccaaa tcctggtcag cgaagcgacc
     1141 ctcgcgcgac tcggcggccg cttcgaagtc gaagagctcc cgcccgccca actgaagggc
     1201 aaagaaaagc ccttccgcgt cttcaatgtg acgcgcatgc gcccgagcgt tcaggtcgcc
     1261 caagcaatcg gcacctgagc tcgaattcgc cgaagggacc tcgtctcctc tgcgaagcaa
     1321 cggagcgcgc ccggtgccgc cacgcgcgag cgacccggcg cgccaaccgg gccgatcgaa
     1381 ggcgcagccc gcgctcggtc atttcgtgac cagacgggtg aaagcctcgt ggtaggcctc
     1441 tgctcctgta tgaagttcgt ccacgccgcc gatttgcaca tcgatagccc gctccgcggg
     1501 ctcgagcgct atcaaggcgc gccggtggag atcattcgtg gcgccacccg acgcgcggtc
     1561 gaacacctcg tcgacctctg tctcgaagaa ggcgtcaagc tcctggtttt gtgcggagat
     1621 ctctacgacg gcgattggaa ggattattcg accgggctct tcttttgtca gcagatggcg
     1681 cggctacgag aagccgacgt cgtggtcgca tggatccgtg gcaaccacga cgccgccagt
     1741 cggctgaccc agcacctccg gcccaccgac aatgtccacg agctgtccca cgctcgtccc
     1801 atcacgctcg aacgcgagat cgacggtgtt tcggtcgctc ttcacgggca gggcttttcc
     1861 aaggcaaagg tcacggaaga tctcaccgat cgttatcccg ccccggtctc gggagccttc
     1921 aatctgggcc tcttgcacac cgcgctcgac ggccgcgagg ggcacgatgc gtacgcgccc
     1981 tgccgtgtcg agtcgctgat caacaagggc tacgactact gggcgcttgg ccacgtgcat
     2041 cagcgggagc aggtgaatcg cgaaccgtgg atcatctttc ccggcaattt gcagggacgc
     2101 cacgcgcgcg agaccgggaa gaagggcgcc acactggtga ccgtcagcgg cggccgcgtg
     2161 accgaggtcg aggctcgtgc gctcgatgag gtgcgctggg ctcacgtcgt ctac
//
LOCUS       DASTKS010000559         6898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_89072,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000559 DASTKS010000000
VERSION     DASTKS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89072"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VFQ35_11495"
     CDS             <1..1658
                     /locus_tag="VFQ35_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931308.1"
                     /translation="RDMWEKIVLNLVSNAFKFTFEGKVTVSLSAIDGRAVLRVRDTGI
                     GVPPEELPHLFERFHRVTGAKSRSHEGTGIGLALVHELVRMHQGTIDVESTVGVGTTF
                     SVSLPLGKDHLPAERLQPARPALHASIGAAAYAQEALRWLPGDTGSTAPGLEHPALGA
                     FGVPPPSLGRPARILFADDNADMREYVGRLLRNHWRVEVVADGRAALEAARREPPDVV
                     LSDVMMPELDGFELLAALRADERTRTVPVVLLSARAGEEATIEGLSAGADDYLVKPFG
                     ARELIARVRATLTLSRLRAEAKEQAENSARIEREARAEAEALARALERSNAELDQFAY
                     VASHDLKAPLRGIANLSQWIEEDVGASLSGESREHMALLRGRVERLEHLIDGILAYSR
                     AGRVRANNEWIDVGKVLTESVELLAPPTHVRVQIEPNMPTLEAERVPFQQVFLNLIGN
                     AIKYTRRADALVRVGCRDEDEYYHFTVADNGPGISPDYHQRIWGIFQTLAPRDEVEGT
                     GIGLSVVKKIVETRGGRTWVESAEGHGATFHVLWPKRPRGGVS"
     gene            1655..2074
                     /locus_tag="VFQ35_11500"
     CDS             1655..2074
                     /locus_tag="VFQ35_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931309.1"
                     /translation="MSEPKLLNILLVEDDKVDVKNVERALKRNNISNPLFVASNGLEA
                     LRMLRDGTVPGTRRLILLDLNMPKMNGIEFLRELRADPALAHSNVVVLTTSNDDQDKL
                     AAYDLHVAGYLLKPVTFVNFVELMAALNKYWALVEMP"
     gene            2071..4197
                     /locus_tag="VFQ35_11505"
     CDS             2071..4197
                     /locus_tag="VFQ35_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931310.1"
                     /translation="MTDQKQERLSLLVVDDDAVDRAAVKRALRGTDLDVEVMEVADAP
                     EALEALRRGGHDCALLDFRLPRGDGLALLRAARSEGLQTPIVVLTGQGDETLAVEIMK
                     AGALDYLAKNAVTPDRLRQSIVQAVRVRRAEVVADLERKRLARLHAFVAQLATTQTVT
                     EVAAAVLTEGLAVFDATRGIVALLTSDGDALDIVDARGYPDEAISVWRQTPLTSDAPL
                     TNAVRERELQFVESRAAFDARYPHLREHRSGQSEAVAVIPLVAGRVLGSVALSHERAF
                     TLGAEQRGELLAFGRVCAQAMARAQLFELAQLERNRAEEASRAKDDFLAVVSHELRTP
                     LNAILGWTRLLRTGNLSDSRGRQALEVIERNATAQAQLIEDLLDISRITAGKLRLQLR
                     QFDLHEVVKGALDVVRPAADAKSITLRSTIDDSVGPLMGDPDRLQQVVWNFLTNAVKF
                     TPKGGKVWVCVRPVDSQVEVEVSDTGCGIAPAFLPHVFERFRQAEAGTTRSHSGLGLG
                     LAIVKSLVELHGGTVEARSEGEGRGTTFIARLPVAPIRLEDTRRPSVHPRAPGVAPEF
                     ACPPQLASVRVLIVDDEPDGRDLIFAALSECGAEVVGAASAAEALSRLSNERFDVLVS
                     DIGMPGEDGYALIAKVRALGPESGGDMPAVALTAFARGEDRTRALAAGFDVHVAKPVE
                     PAELVLILARLVERRRPSTLPPLAPK"
     gene            complement(4314..5531)
                     /locus_tag="VFQ35_11510"
     CDS             complement(4314..5531)
                     /locus_tag="VFQ35_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ricin and poly(3-hydroxybutyrate) depolymerase
                     fusion"
                     /protein_id="HET9931311.1"
                     /translation="MQLHVDRRFSALAFVTALLSWTGCSNSAGNSGGNGGSSTSSGGA
                     SSSGGASNGGQVTGSGGSVTSGGQVSSGGSSTSSGGTSSGTGRGGGSSTTGGEQSSGA
                     GRGGTSSGQGGGASGGRSAGTGGAKANGGSTGTAGAAGAGNTTSPAPSSGCGKAAGLM
                     NGRATIDSGGKTREYIIALPDNYDQNHPYRLIFGWHPWGGSAQQVASGGYYGLQKLSS
                     GSAIFVAAEGLDFGGNGLGWGNSNGEDIALLGDMLTRFRSQLCIDENRIFSVGFSFGG
                     MFSFAAGCSSTSMMRAIAPMAGNTSVAGCGSGTRPVAIMGFHGVDDQVVAIDGGRKGR
                     DVFVQRNHCTSETVPADTTWCSSAGQNYQPCSCVSYKGCMEGYPVTWCEYKGQHMPAP
                     NSAQTLWTFFSQF"
     gene            complement(5819..>6898)
                     /locus_tag="VFQ35_11515"
     CDS             complement(5819..>6898)
                     /locus_tag="VFQ35_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931312.1"
                     /translation="GERALGGQSGVETGGSLGTNGGSAHSGGDTSLGGTGKGAGGSLG
                     TGGTNVVAGASSGGRASGGQPGSSGATGNGGSAGGAVAGAGGGSGRPIPLGNPAVPSS
                     GCGKAATLTSGKKTITSGGQQRTYIIDIPANYDMNKPYRLFYTSHWIGSTSEAVRDQN
                     YYFLKPLANAANAPAVFVAPQADGSTWQQKDHALFDDILAFVKSNLCIDTTRVFATGF
                     SFGGMITYSLSVNHQKDIRAAVGIAPANYNIYVPQKNHEPIAWMQTTGMSDTTCPWIN
                     GSSTTQGAKFIAIEHATDNRCTVPATLPIWQQGKHVCYDFQGCGAEYPTKVCTFGGGH
                     TNIANDPGASANWIPEESWKFFTQF"
BASE COUNT         1217 a         2334 c         2178 g         1169 t
ORIGIN      
        1 atcgcgacat gtgggaaaag atcgttttga acttggtttc taacgctttc aagttcacct
       61 tcgagggtaa agtcacggta tccctctcgg cgatcgacgg ccgggcggtg ctgcgcgtgc
      121 gagacaccgg catcggcgtc ccacccgaag agctgcccca cctgttcgaa cgctttcacc
      181 gagtgaccgg cgccaagtcg cgctcgcacg aagggaccgg tatcgggctc gcgctcgttc
      241 atgaactcgt tcgcatgcac caaggcacga tcgacgtaga gagcacggtc ggggtcggaa
      301 cgacgttcag cgtaagccta cccctcggca aagaccatct cccggccgaa cgattgcaac
      361 cggcgcgccc tgcccttcac gcttcgatcg gtgccgccgc ctacgcgcaa gaggcgctac
      421 gctggctacc gggcgatacg ggctcaactg cgcccggcct cgagcaccca gcgctgggcg
      481 cttttggcgt gccgccaccg tcgctggggc ggcccgcgcg catcttgttc gccgacgaca
      541 acgccgatat gcgcgagtac gtcggccgtc tgttacgcaa tcattggagg gtcgaggtcg
      601 tcgccgacgg ccgcgcggcc ctcgaggcgg cgcgtcgcga gccgcccgac gtcgtgttga
      661 gcgacgtcat gatgccagag ctcgatgggt tcgagctgct cgcggccctg cgcgcggacg
      721 agcggacgcg cacggttccg gtggtcctgc tctcggctcg cgccggcgaa gaagcgacga
      781 tcgaaggcct gagcgccggg gccgacgact acctcgtgaa gccgttcggc gcacgcgagc
      841 tcatcgcgcg ggtcagagcc acgctgaccc tgagccgcct ccgtgccgaa gccaaagagc
      901 aagccgagaa ttcggcgcgc atcgagcgcg aggcgcgcgc ggaggcagaa gcgctggcgc
      961 gagcactcga gcgctcgaac gcggagctcg accagttcgc ttacgtcgcg agccacgact
     1021 tgaaggcgcc gctccggggc atcgcgaacc tctcgcaatg gatcgaagag gacgtcgggg
     1081 cctcgctctc gggcgaaagc cgcgagcaca tggcgctcct gcgaggccgc gtcgaacgcc
     1141 tcgagcacct catcgacggg atcctcgcct actcgcgcgc gggtcgagtg cgcgcgaaca
     1201 acgaatggat cgacgtcggg aaagttctga ccgaatcggt cgagctgctc gcgcccccta
     1261 cccacgtccg cgtgcaaatc gaaccgaaca tgccgaccct agaggccgaa cgagtgcctt
     1321 tccagcaggt atttttgaac ctgataggta acgccatcaa gtacacgcga agagcagacg
     1381 cgctcgttcg agtcggctgc cgtgacgagg acgagtacta tcacttcacc gtcgccgaca
     1441 acggacccgg gatctcgccc gactaccatc aacgcatttg gggaattttt cagacgctcg
     1501 cgccgcgcga cgaagtcgaa ggcacgggga tcggtctatc ggtcgtgaaa aagatcgtgg
     1561 aaacgcgcgg agggcgcacc tgggtcgagt ccgccgaagg ccacggagcg accttccacg
     1621 tcctttggcc caagcgtccg cgtggaggcg tctcgtgagc gagcccaaac tgctgaacat
     1681 cctgctcgtc gaggacgaca aggtcgacgt caagaacgtc gagcgagcgt tgaagaggaa
     1741 caacatatcg aacccgctct tcgtggcttc gaacggcctc gaggcccttc gaatgttgcg
     1801 agacggcacc gtcccgggca cgcgccgcct catcttgctg gacttgaaca tgccgaagat
     1861 gaacggcatc gagttcctgc gcgagcttcg cgcggaccct gccctggccc attcgaacgt
     1921 cgtggtcttg acgacatcca acgacgacca agacaagctc gccgcttacg atctgcacgt
     1981 cgccgggtac ctgctcaaac cggtgacgtt cgtcaatttc gtggaattga tggccgctct
     2041 caacaaatac tgggcgctcg tcgagatgcc atgaccgacc agaagcaaga gcgcctttcg
     2101 cttttggtgg tcgacgacga cgcggtcgac cgcgccgcgg tgaaacgcgc gcttcgggga
     2161 acggatctcg acgtcgaagt catggaggtc gcggacgccc cagaggcgct cgaagcactc
     2221 cgtcgcggcg gccacgactg cgcgctgctc gactttcggc ttccgcgcgg cgacgggctc
     2281 gctctgcttc gagccgcccg cagcgagggg ctccagacgc ccatcgtggt gctcacgggt
     2341 caaggcgacg agacgctcgc ggtcgagatc atgaaagcgg gggccctgga ctatctcgcg
     2401 aagaacgccg tcacgcccga ccgcctacgg cagagcatcg tgcaagcggt gcgcgtgcgt
     2461 cgtgcggagg tggtggccga tctcgagcgc aagcggctgg cgcggctgca cgccttcgtc
     2521 gcgcagctcg ccactacgca gacggtgacc gaagtcgcgg cggcagtgtt gacggaaggg
     2581 ctcgccgtgt tcgacgcgac tcgaggcatc gtcgcgctgc tcacgagcga cggcgacgcg
     2641 ctcgacatcg tggacgcacg cggctatccg gacgaagcaa tcagcgtttg gcgccaaact
     2701 ccgctgacga gcgacgctcc gctcacgaac gcggtccgcg agcgcgagct tcagttcgtc
     2761 gagagccgcg ccgccttcga cgcccgttat ccccacttac gcgagcatcg ttcgggccag
     2821 agcgaggccg ttgccgtcat cccgttggtc gccggtcgag ttctcgggag cgtcgcactc
     2881 tctcacgaac gagccttcac cctgggtgcg gaacagcgcg gagagctgct cgcgttcggt
     2941 cgggtctgcg cacaagccat ggctcgtgcc cagctattcg agttggctca gctcgaacgc
     3001 aaccgcgccg aagaggcaag ccgagccaaa gacgacttct tggcggtagt ttcgcacgaa
     3061 ctcagaactc ccttgaacgc catcctcggc tggacccgct tactcaggac tggaaacctc
     3121 agcgactccc ggggcaggca agcgctcgag gtgatcgagc gcaacgcgac cgcccaagcg
     3181 cagctcatcg aggacttgct cgacatcagc cgcatcaccg cagggaagct gcggctccag
     3241 ctgcggcagt tcgacctgca cgaggtcgtc aaaggagcgc tcgacgtcgt gcgcccggcc
     3301 gccgacgcca agagcatcac gctgcgctcg acgatcgacg attcggtcgg gcccttgatg
     3361 ggtgatccgg atcgcttgca acaggtggtt tggaacttcc tcacgaacgc ggtcaagttc
     3421 acgccgaaag gcgggaaggt ttgggtctgc gtgcgtcccg tcgactccca ggtcgaagtc
     3481 gaagtgagcg acaccggttg cggcatcgcc cccgccttcc tccctcacgt attcgagcga
     3541 ttccgccaag ccgaggcagg cacgacgcgc tcccacagtg gtctcgggct cggcctcgct
     3601 atcgtaaaga gcctcgtgga gctgcacggc ggcacggtcg aggcgaggag cgagggcgag
     3661 ggccgcggaa cgacgttcat cgcccgccta ccagtcgcgc cgattcgtct cgaagacacg
     3721 cggcgccctt cggttcatcc ccgggcgccc ggcgtcgcgc cagagtttgc gtgcccgccg
     3781 cagctcgcct ccgttcgcgt gctcatcgtc gacgacgaac ctgacgggcg agacctgatc
     3841 ttcgctgcgc tttccgaatg cggcgccgag gtggtcggcg ctgcgagcgc ggccgaggcg
     3901 ttgagccggc tatcgaacga gcgcttcgac gtgctcgtga gcgacattgg catgcctggc
     3961 gaagacggct atgcgctcat cgcgaaagtc agagctctcg gccctgaaag cggaggggac
     4021 atgccggcag tcgcgctgac cgcgtttgcg cgaggagaag accgcactcg cgcacttgcg
     4081 gccgggttcg acgtccacgt cgcgaaaccg gtcgagccag ccgagctggt gctcatcctc
     4141 gctcggctgg tagagcgccg gcggccgagt acgctgccgc cgctcgcgcc aaagtagagc
     4201 gccgcgcagc cgagtacgct gccgccgctc gccctaaagc gcgcgcaggc gactaccctg
     4261 ccgctccgct tcgaagccgc agggtctcga ctcccgcaga gcggagaact gcctcagaac
     4321 tgggagaaga aggtccagag ggtctgcgcc gaattgggtg ccggcatgtg ctggcccttg
     4381 tattcacacc aggtgaccgg gtagccctcc atgcagccct tgtagctcac gcagctgcac
     4441 ggctgatagt tttggccagc ggagctacac caggtcgtgt ccgcgggaac ggtctccgag
     4501 gtgcagtggt tgcgttggac gaagacgtcg cggcccttgc ggccaccatc gattgcaacc
     4561 acctgatcat cgacgccatg gaagcccatg atcgccaccg ggcgcgtgcc acttccacaa
     4621 ccggcgacgg aggtgttgcc agccatcggt gcgatcgcac gcatcatgct cgttgacgag
     4681 caccccgcgg cgaacgaaaa catgccgccg aagctgaacc cgaccgaaaa gatcctgttt
     4741 tcgtcgatgc agagctgcga tcggaaccgc gttagcatgt cgccgagcaa cgcgatgtcc
     4801 tcgccgttcg agttgcccca gcctagaccg ttgccgccaa agtcgagccc ttcggccgcg
     4861 acgaagatgg cgctcccgct cgatagcttc tgaaggccat agtagccgcc gctggcgacc
     4921 tgctgagcgg agccgcccca gggatgccag ccgaagatca gacgatatgg gtggttctga
     4981 tcatagttgt ccggcaacgc gatgatgtac tcgcgggtct tgccacccga gtcgatggtg
     5041 gcgcgcccgt tcatcagacc tgctgccttg ccgcagccag agctcggcgc gggactggtg
     5101 gtgtttcctg cacccgcggc cccggcggtt cccgtggaac ccccgttggc cttcgcaccg
     5161 cctgtgccag cagagcggcc gccagacgcg ccccctccct ggccagagct cgtgccgccg
     5221 cgtccggccc ctgagctctg ctctccgccg gtcgtgctac tgccgccgcc gcgaccagtg
     5281 ccggaggagg tccccccgga gctcgtggag ctaccgccac tgctcacctg tccgccgctg
     5341 gtgacgctgc cgcccgagcc ggtcacctgc ccgccattcg aagccccgcc cgaactcgag
     5401 gcgccgccac tcgacgtgct cgagccaccg ttgcctccgg aattcccggc agaattcgag
     5461 cagcccgtcc acgagagcaa agccgtcacg aacgcgagcg cagaaaacct acgatcaaca
     5521 tgcagctgca tgactatctc cctgagaact cgaactctcg agcaatcagc aattccgcga
     5581 cagctccagg ccgccgcggg tgcgcgtgat gagcacaggg tagcccaggc acaggacatg
     5641 acgagtgggc acttctgccc cctgcggatc atcgtaagta tccaccgggg ctgtcatgtc
     5701 gcgaaagcca tgcgtcgcgc ccgtcggagg ggaccacgcg gcgcccagct aaaccgcgcc
     5761 cagccgtgca agaccctgcg cgtcgcgtaa ggaccgcgca cgaatggtct cgaacccatc
     5821 agaactgcgt gaagaacttc caggactctt ccggaatcca gttcgcgctg gcaccgggat
     5881 cattcgcgat gttcgtgtgg ccgccaccga acgtgcacac cttcgtcggg tattcggcgc
     5941 cacaaccctg gaagtcgtaa cacacgtgct tgccttgctg ccaaattggg agcgtggcgg
     6001 ggaccgtgca gcggttatcc gtcgcgtgct cgatcgcgat gaacttcgcc ccctgggtcg
     6061 tgctgctccc gttgatccac ggacaagtcg tgtcgctcat cccggtcgtc tgcatccacg
     6121 cgatgggttc atggttcttc tggggcacgt agatattgta gttggcggga gcgatcccca
     6181 ccgcggcccg gatgtccttc tgatggttca ctgacaagga gtacgtaatc atccccccga
     6241 agctgaaccc ggtcgcgaag acgcgtgtcg tgtcgatgca caggttcgac ttcacgaacg
     6301 cgaggatgtc gtcgaagaga gcgtggtctt tctgttgcca cgtggaaccg tcggcttgtg
     6361 gtgcgacgaa aacggctggc gcgttcgcgg cgttggcgag aggcttcaag aagtaatagt
     6421 tctgatcgcg aactgcctcc gacgtgctgc cgatccaatg cgacgtgtaa aacagccggt
     6481 acggcttgtt catgtcgtag ttcgcgggaa tgtcgatgat gtacgttcgc tgttgacccc
     6541 cgctcgtaat cgtctttttc ccgctcgtta gcgtggccgc ttttccgcac ccgctgctcg
     6601 gaacagctgg atttccgagc ggtatcggtc ggccgctgcc gccccctgct cccgctactg
     6661 cgccccctgc cgaaccaccg ttacccgttg cgccgctcga gccgggttgg ccaccgctcg
     6721 ccctgccgcc cgacgacgcg ccggcgacga cgttcgtccc gcccgtccca agcgaacctc
     6781 ccgcgccctt ccctgtgccg ccaaggctcg tatcgcctcc cgagtgagcg ctgccaccat
     6841 tcgtcccgag cgagcccccc gtctcgaccc ccgattgccc acccaaagcc cgctcgcc
//
LOCUS       DASTKS010000560         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_89399,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000560 DASTKS010000000
VERSION     DASTKS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89399"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFQ35_11520"
     CDS             <1..344
                     /locus_tag="VFQ35_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931313.1"
                     /translation="AHVASKAHRAARRRVETPMENVDESESDSPLQDALLDERRARDL
                     LDEFLSELADDVRAVFVLFEIEGLRAPEVAEALEIPVGTVASRLRRARAEISAKLSRR
                     EARSAFKGRRA"
     gene            341..1174
                     /locus_tag="VFQ35_11525"
     CDS             341..1174
                     /locus_tag="VFQ35_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931314.1"
                     /translation="MTELKRWEQDGASDAIARLLQAARTELPSEASLAQSIAAAGAGA
                     VAATATTTAQAALGKLGASTLLGAKAPGLLPALGVLKWLLVGTALGSVVMATLPSTRQ
                     DSPSAMAPSSHTARVGFVPVQSSRTLAPVAPEASSSPAIVAPLATVPARAASSEPART
                     TLERALPLADTRRTGTSRGAAPLIESERLAEEVRAIDDATRALGSGQATRALALLDDY
                     DRRYAERRFAPEALYLRMEALTRLGRSGEARTVAKRLANAYSTSPQAARARALLGETI
                     R"
     gene            1242..2225
                     /locus_tag="VFQ35_11530"
     CDS             1242..2225
                     /locus_tag="VFQ35_11530"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET9931315.1"
                     /translation="MKRSIALTSFAFSIFGTACGGFTEIGEGTAGSDSGGATAQGGKS
                     PGGSPGTGGARFTGGASGLGGAAAGGTTADGGSAGIGNASSTGGSAGSVGQPCTTSEE
                     CPQIKAPCRLCEDGSEACPWSRCENGVCLAGIDQCPTPNPCAGKKCGDICTTCSPGQT
                     CPPIVEYCDSKQQCQRDQPNCGGSCSATGESCANGETCCDGLECCVGVPVPQGSEFCG
                     TVCPKSDQNIKRNFLSVDPDQVLDKLSRLPVGTWSYRTESSTERHIGPMAQDFMAAFH
                     VGSSDRTILQVDADGVAFAAIQALNARLKSLEERNQKLERDLAEQRSRCER"
     gene            complement(2222..3271)
                     /locus_tag="VFQ35_11535"
     CDS             complement(2222..3271)
                     /locus_tag="VFQ35_11535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9931316.1"
                     /translation="MTTLELGLDTFGDRTRGADGQLESHAEVTRNLVEQAKLADRVGL
                     AFIGVGEHHRDDFAISAPEVVLAAIAGQTSRIHLGSAVTVLSSDDPIRVFQRFSTLDA
                     LSNGRAEVILGRGSFTESFPLFGFPLERYQELFEDKLALFAKVRSGNPVTWSGSTRTP
                     LENQVVYPPVEKGLLRTWIGVGGSPESVVRAARYGFDLMLAIIGGEPARFAPYVALYK
                     EALTQFGFGARPIGVHAPGHLADTDERAREELWPHYLEMRTKIGRERGWPPPSRAQFE
                     AEAGPRGALFVGSPETVAQKIVATARTLSLSRFDLKYSNGPMPHEKLMQSIELMGTKV
                     APRVQELLAADAVAP"
     gene            3452..>3648
                     /locus_tag="VFQ35_11540"
     CDS             3452..>3648
                     /locus_tag="VFQ35_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET9931317.1"
                     /translation="MPSKQTLQRAKEDRKEGKSASTQAGEFIREEMHHIREGKHGARN
                     AKQAIAIGLSKARRAGFGAPPG"
BASE COUNT          683 a         1179 c         1208 g          578 t
ORIGIN      
        1 cagctcatgt cgcttccaaa gcgcatcgcg cggctcgaag acgggtggag acgccgatgg
       61 aaaacgtgga tgagagcgaa agcgacagcc cgttgcaaga cgctttgctc gacgaacggc
      121 gcgcgagaga cctgctcgac gaattcttga gcgagcttgc cgacgacgtt cgcgccgtct
      181 tcgttttgtt cgaaatcgag gggcttcggg cgcccgaggt cgcggaagcg ctcgagatcc
      241 cggtgggcac ggtcgcgtcc cggttgcggc gcgcgcgagc cgaaatcagc gcgaagctgt
      301 cccggcgcga ggcccgctcc gctttcaaag gacggcgagc atgaccgagc tgaaacgctg
      361 ggaacaggac ggcgcctcgg acgccatcgc tcgcttgttg caggcggcgc gcaccgagct
      421 gccatccgaa gcatctctcg cgcaaagtat cgcggcggct ggggccggcg ccgtagcagc
      481 gaccgcgacg accacggccc aggctgctct cggcaaactc ggagcatcga ctttgctcgg
      541 cgcgaaagcg ccggggctct tgcccgctct cggcgtgctc aagtggcttc tagtcgggac
      601 cgcgctcgga agcgtcgtca tggctacctt gccctctact cgccaagaca gcccatcggc
      661 gatggctccg agcagccaca ccgcgcgcgt cggtttcgta ccggtgcagt cgtcgcgaac
      721 gctagccccg gtcgcgcccg aggcatcgtc ttcgcctgcg atcgtcgctc cgttggcgac
      781 ggtgccggct cgagccgcct cgtcggagcc cgctcgaacg acgctggagc gagctttgcc
      841 gctagccgat acacggcgca cggggacttc gcgcggagcc gcaccgctca tcgagtcgga
      901 gcgactcgcg gaagaagtgc gagccatcga cgatgcaacg cgggcactcg gaagtggaca
      961 ggcgacgcgc gcacttgcgc tcctcgatga ctacgatcgc cgctatgccg agcggcggtt
     1021 tgcgccggag gccctgtact tgcggatgga agcgctcacc cggctcggac ggagcggcga
     1081 agcccgcaca gtcgccaagc gattggcgaa cgcatattct acgagtccgc aggctgcgcg
     1141 cgctcgtgcg ctactcggcg agacgattcg gtgatcgaga ggcgtcgccc cggggatagg
     1201 cgccggaaca gacaaacgtt cggggacagg aggagtcgat catgaaacgc tcaattgcac
     1261 tcacgtcgtt cgcgttcagc atcttcggca cggcctgcgg gggctttacc gaaatcggcg
     1321 aaggcaccgc tggcagcgat agcggcggcg cgacggcgca aggaggcaaa tccccgggcg
     1381 gctcaccggg cacgggcggc gcgcgcttca ccggtggagc gagcgggctt ggcggtgcag
     1441 ctgcgggcgg gactacggct gacggcggaa gtgcaggaat cggcaacgcc tcgtccaccg
     1501 gagggagcgc cggaagcgtc gggcaaccgt gcaccacgtc cgaggaatgc ccgcagatca
     1561 aggcgccctg cagattgtgt gaggacggct ccgaggcgtg tccgtggtcg cgctgtgaaa
     1621 atggggtttg cctcgccggc atcgaccaat gcccgacccc gaacccctgc gcgggcaaga
     1681 agtgcggaga catctgtacg acgtgcagcc ccggccaaac ctgtcccccc atcgtcgaat
     1741 attgcgattc aaagcaacaa tgccagcgtg accagccgaa ttgcggcggc agctgctccg
     1801 ccaccggcga gagctgcgcc aatggcgaaa cgtgctgcga cgggctcgaa tgttgcgttg
     1861 gcgtacccgt ccctcaaggc agcgagtttt gcggcaccgt gtgtccgaag agcgaccaaa
     1921 acatcaagcg caacttccta tcggtggacc ccgatcaggt gctcgacaag ctgtcgcgtc
     1981 tacccgtcgg cacttggtcg tatcgcaccg agtcgtcgac cgagcgccat atcgggccca
     2041 tggctcaaga cttcatggcg gcgtttcacg ttggctcgag tgaccgcacg atcttgcagg
     2101 tcgacgcgga tggcgttgcg ttcgccgcaa tccaagcgct gaacgccagg ctcaagtcgc
     2161 tcgaagagcg caaccaaaag ctggaacgcg acctcgcgga gcagcgttca cgttgcgagc
     2221 gctagggcgc gaccgcatcg gccgcgagga gctcttggac cctcggcgcg accttggttc
     2281 ccatgagctc gatgctctgc atcagctttt cgtgaggcat cgggccgtta ctgtacttga
     2341 gatcgaagcg agaaagagac agcgtccgtg ccgtcgcgac gatcttctgc gcgaccgttt
     2401 cgggtgagcc gacgaagagc gctccgcgcg ggccggcttc ggcctcgaac tgcgcgcgcg
     2461 aaggcggcgg ccagccccgc tcgcggccaa tcttcgttcg catttcgagg tagtgcggcc
     2521 aaagctcctc gcgcgctcgc tcgtccgtgt ccgccaagtg ccccggtgcg tggaccccaa
     2581 tcgggcgcgc cccaaatccg aactgtgtca gcgcctcctt gtagagagca acgtaaggcg
     2641 caaagcgggc gggctctccg ccgatgatcg ccagcatcag gtcgaagcca tagcgcgccg
     2701 cgcgaaccac cgactccggg ctacccccaa cgccgatcca ggtgcgcagc agccccttct
     2761 cgaccggggg atacaccacc tggttctcga gcggggtgcg agtcgagccc gaccacgtga
     2821 cggggtttcc ggagcgcacc ttggcgaaca acgcgagctt gtcctcgaat agctcctggt
     2881 aacgctcgag cgggaagccg aacaatggga acgactcggt gaacgagccg cggccgagta
     2941 tcacctccgc gcgtccgttc gagagcgcgt cgagcgtcga aaaccgctga aacactcgaa
     3001 tcggatcgtc ggagctcaac accgtgaccg ccgaacccaa gtgaatgcgc gaggtctgac
     3061 cagcaattgc ggcgagaacg acttcggggg cagagatcgc aaagtcgtcg cgatgatgct
     3121 ccccgacgcc aatgaacgcg agccccacgc gatccgcaag cttcgcctgc tcgaccaagt
     3181 tccgcgtgac ctcggcgtgt gactcgagtt gcccatcggc gccacgcgtg cggtcgccaa
     3241 aggtgtcgag gccgagctcc aaggtcgtca cttcgttcct cgtttcgcga ggacggtacg
     3301 gagggtcgtc atcgccccgt cgtaccattc ggcgcgaccg ggcggcatct gactctgcag
     3361 aacggcgcga aacgtcacga cgcgaagcca agagcaacgt tgcggggtcg ctcgactgaa
     3421 agctggcctg cttgttgctt tcgactcacc tatgccgagt aaacagactc tacagcgggc
     3481 caaggaagat cgaaaagagg gcaagagcgc gtcgactcag gctggtgagt tcattcgcga
     3541 ggagatgcac cacatccgtg aaggtaagca cggcgctcga aacgcgaagc aggcgatagc
     3601 gattgggctc tccaaggcgc gccgagctgg ctttggcgct ccgccagg
//
LOCUS       DASTKS010000561         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_312205, whole genome shotgun sequence.
ACCESSION   DASTKS010000561 DASTKS010000000
VERSION     DASTKS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312205"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1195
                     /locus_tag="VFQ35_11545"
     CDS             335..1195
                     /locus_tag="VFQ35_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931318.1"
                     /translation="MLPERTLTPRFVAMSRLLAATACSLPTVSAWASEPTVSECLAAS
                     EASLTAGNEHRRLAERAELSRCASPSCPAVVREECTRRLAEVARLIPSVVFDIRGTKP
                     GALSGLSVSVDGHAVEASAMGSPMALEPGEHSVVVRAQGYAVESQQITVREGESKREI
                     HVQLVRASDQMREKEPPAIESAGRRTQRVAAYGAAGVAAVGLGVGTAFGVLAWSKKRK
                     AGEVCPTRCRDDAGVEAWEDARRAGNISTVAFAVGGVAVVAAAALWITLPGDTQVAIG
                     LDDISLRGHW"
     gene            1192..1869
                     /locus_tag="VFQ35_11550"
     CDS             1192..1869
                     /locus_tag="VFQ35_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931319.1"
                     /translation="MMTLVGRVCAAALGLVLLANCNAVLGIERHERDEAHGGNGAEAA
                     GGAFSAGGSSSVAASGNADFDGGAAGGSPDSANGGVTSLNGGVTSVSGGVTSVNGGGS
                     DATGGAPASGGASPNSCTPACAASQVCEGARCVECRNAERSCAADGTPLLCAGNVWSK
                     QSACAGTTPVCSHGTCVGTRLVGGIVSVGGTLSRAGVQLVEQSLELTAPACSTVVGKC
                     VVGGIRP"
     gene            1866..2660
                     /locus_tag="VFQ35_11555"
     CDS             1866..2660
                     /locus_tag="VFQ35_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931320.1"
                     /translation="MRRWLFTRTTRLTKSMKWVLPAATVMLCLLGAFLAGKSFAAGIP
                     PTGALTYSGLLQDAGGQPLAGPQFVEVKLWNHPSANANTNLLCDSGTPASVQLVSGHF
                     SLVLPDTCSTAVAANPDVYTEVFVGASAAGAASLGRSKLGAVPYAISAENSTRATVAS
                     TADTANVASSFAARTKPFPGATPSATTAYKLQSGSTVAKIEANGRVRIPFVEAFPKGL
                     VSVVATDSYAGSRPVFGLDDDEDLTGFTVIGTPNATIRVNWIAIGW"
     gene            complement(2685..3623)
                     /locus_tag="VFQ35_11560"
     CDS             complement(2685..3623)
                     /locus_tag="VFQ35_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931321.1"
                     /translation="MTEASLVPLLATGDDEAHQRFVDLVAEVRPELFKYCARMTGSIF
                     DGEDIVQETLARAYAALGNATEPPPLRPWLFRVAHNAAMDFLKRYERKHVDFVAEAPE
                     PVEPDVNDVDPALVEAALVRFMDLPLLQRSALVLKDVLGHSLAETAETMGISVSAVKA
                     ALVRARENLSQNTPRREPRERSVSAEEHQKLRQYAELFNNRDWDGLRALMGEQSRLEV
                     MTRVRRRGRAAAEYYTRYAEVAGPEALRAEVGWVDTTPVIAIFRPASAATPAYFILIE
                     WDGEKVSLIRDFRYVPYLTDAAEYASAENATPPEHA"
     gene            complement(3620..4369)
                     /locus_tag="VFQ35_11565"
     CDS             complement(3620..4369)
                     /locus_tag="VFQ35_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9931322.1"
                     /translation="MNLQGKKAIVIGASTGVGRAVTESLLSQGARVTAVARAAEGLRA
                     LQSELGSPIDVRQADAAEPSTAQRLLAELQPDYVVLSAGVRPQMGAIDELSWETFSEA
                     WTSDTKAAFHLVQAALRLPLRPGSVVAVVSSGAAINGSYLSGGYAGAKRMQWLLAGYA
                     QKIADERKLGIRFLAVVPKQLIEGTVIATVASERYARALSISPAAYMKRFEVPLHCDQ
                     VAAGIVTGLRGELAPHLTAIGVTGTGIEPLA"
     gene            complement(4501..5061)
                     /gene="orn"
                     /locus_tag="VFQ35_11570"
     CDS             complement(4501..5061)
                     /gene="orn"
                     /locus_tag="VFQ35_11570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET9931323.1"
                     /translation="MPPFVKRQQSATNLVWLDLEMTGLDPVRDVILQAAIVITSAALE
                     PLEEFACDVWQPASALEHMTPYVRDMHEKNGLLARVAASRTDLFAAEKQLLDRIAGWC
                     TYPALLCGNTIGQDKRFIDRYMPGLSGYLSYRSVDVSSIKMLAKLWYGDAAMFAKPTD
                     AEHDALFDVKQSVAELAHYRATLFRK"
     gene            complement(5063..>5245)
                     /locus_tag="VFQ35_11575"
     CDS             complement(5063..>5245)
                     /locus_tag="VFQ35_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931324.1"
                     /translation="AVAQVIGHTLLVYRRRKKDPKIQLPKDKPGSKAKAANQATSANK
                     SQPARKGGPASNDEEE"
BASE COUNT          865 a         1644 c         1812 g          924 t
ORIGIN      
        1 gtcacgcccg ggtttcagcg gccagtgccg gaaacacctc ggcgacggac gcttccgcgc
       61 gcgctttgcc tctcgtcgga cccgctggca tggcgccaag ctcggtatcg ccgcgcgagc
      121 ttccaagtgt gaccgagccg ggcgagggtc tcgccgcttc cgggtcaact cgttcgagcg
      181 cggtgcggcg agcgacgccc aactttggtg gtcggcttgg gtccgcgatg agcgctgcgc
      241 ccctgccgga tgcagcgcag cgccggagcg attcggtcgg ttgtgccccg ccattcgtgg
      301 tcgacgcact cggcaggaaa aggttcaaac cggaatgctt cctgaacgca ccctgacccc
      361 gcggttcgtc gccatgagtc gactgctcgc ggcgaccgcc tgcagcttgc ccacggtttc
      421 ggcttgggcg agtgagccga ccgtgtccga gtgcttggcg gcgagtgagg cgtcgctgac
      481 ggcgggcaat gagcatcggc ggctcgcgga gcgcgctgag ctctcgaggt gcgcatctcc
      541 gagctgtccc gcggtcgtga gggaagagtg cacgcgtcgc ctcgccgaag tcgcacggct
      601 catcccgagc gtcgtgttcg acatccgcgg cacgaagccc ggcgcgctgt cggggttgtc
      661 cgtgagtgtc gacgggcacg ccgtcgaagc gtcagcaatg ggctcgccga tggcgctcga
      721 accaggtgaa cacagcgtgg tcgttcgcgc gcagggctac gccgtcgaat cgcagcagat
      781 caccgtgcgc gagggcgagt cgaagcgcga aatccacgtg cagctcgttc gagcttccga
      841 ccagatgcgc gagaaggagc cgcccgcgat cgagagtgcg gggcggcgca cgcagcgggt
      901 cgcggcctat ggcgcagccg gtgtcgccgc ggtcggcctc ggtgtcggga ccgcctttgg
      961 tgtcctggcc tggtcgaaga agcgaaaggc gggcgaggtg tgtccgaccc gttgccgtga
     1021 tgacgccggt gtcgaagcgt gggaagacgc ccgacgcgcc ggcaacattt caaccgtcgc
     1081 tttcgcggta ggcggcgtcg ccgtcgtcgc ggcggccgcc ctttggatca cccttcccgg
     1141 ggacacccag gtcgcgatcg ggctcgacga catatccctg aggggccact ggtgatgacg
     1201 ctcgtcggca gggtatgcgc cgcagcactg ggactcgtgt tgctcgccaa ctgcaacgca
     1261 gtgttgggga tcgagcgcca cgagcgcgac gaagcgcacg gcgggaatgg agcggaagcc
     1321 gcgggaggag ccttctcggc aggcggtagc tccagcgtcg cggcttccgg caacgccgat
     1381 ttcgacggag gagctgctgg cggttctccg gacagcgcga acggcggcgt cacgtcactg
     1441 aacggcggcg tcacgtcagt gagcggcggc gtcacgtcag tgaacggcgg cggaagcgac
     1501 gcgaccggcg gagcccccgc gagcggcggc gcatccccga actcgtgcac tcccgcgtgt
     1561 gctgcctctc aggtatgcga gggcgctcgt tgcgtggagt gtcgcaacgc cgaacggagc
     1621 tgcgcggcag acggaacgcc gctgctgtgc gcgggaaatg tctggagcaa acagtcggcg
     1681 tgcgcgggaa cgacgccggt ctgcagtcac ggcacttgcg tcggcacgcg cctcgtcggg
     1741 ggcatcgtta gtgttggggg cacgctgagt cgtgccgggg tgcagctcgt ggaacaaagc
     1801 ctcgaactca cggcgcccgc gtgcagcacc gtggttggca agtgtgtggt gggaggtatt
     1861 cgaccatgag aagatggctg ttcacgagga cgacgcggct gaccaaaagc atgaagtggg
     1921 ttctgccggc ggcgacagtg atgctctgcc tgttgggtgc attcttggcc ggcaagagtt
     1981 tcgcggccgg tattcctccg acgggcgcgc tgacgtactc cggcctgctc caagacgccg
     2041 gcgggcaacc tttggcgggg ccgcaattcg tggaggtcaa gctctggaac catccgtctg
     2101 ccaacgcgaa tacgaacctt ttgtgcgaca gcgggacccc ggcttcggtg cagctcgtga
     2161 gcggccactt ctcgctcgtg cttccggaca cgtgcagcac cgcggtcgca gctaatcccg
     2221 acgtctacac ggaggtgttc gtgggcgcga gtgccgccgg cgccgcttcg ctcggtcgct
     2281 ccaagctggg cgcggtgccg tacgcgataa gcgccgagaa cagcacgcgc gcgaccgtcg
     2341 cgtcgacagc agacactgcg aacgtggcga gcagcttcgc cgcgcggacg aagccgtttc
     2401 cgggggcaac tccgagcgca acgacggcct acaaactgca gagcggcagc accgtcgcca
     2461 agatcgaagc caacggtcgc gtccgcattc cattcgtgga ggcatttcca aaaggtctag
     2521 tgagcgtggt cgcgacggat tcctacgccg ggagccgccc cgtgttcggc ctcgacgacg
     2581 acgaagacct gaccggattc actgtgattg gtacaccgaa tgcgacgatc cgtgtgaact
     2641 ggatcgcgat cggttggtga cctcgagagt aggcggcgcg cacgtcacgc gtgttcgggc
     2701 ggcgtcgcgt tttccgcgga agcatactct gcggcgtcgg tcaggtaggg gacgtagcgg
     2761 aagtctctga tcagcgagac cttctcgccg tcccactcga tgagaatgaa ataggcaggt
     2821 gtcgccgccg aggccgggcg aaaaatggcg atgacgggcg tcgtgtcgac ccaaccgact
     2881 tctgccctga gcgcctccgg tcctgcgact tcggcgtagc gtgtgtagta ctcggccgcg
     2941 gcgcgcccac gacgccggac gcgcgtcatt acctcgagcc gcgattgctc acccatcaac
     3001 gctcgcagtc cgtcccagtc gcggttgttg aagagttcgg cgtactgtcg gagcttctga
     3061 tgctcttcgg ccgacacaga acgttctcgg ggttctcgac gcggcgtgtt ctgcgacaag
     3121 ttttcccgcg cgcggaccaa cgccgctttc acggccgaga cgctgatgcc catcgtctcg
     3181 gcggtctctg cgagcgaatg gccgaggacg tccttgagaa cgagcgcgct ccgctggagc
     3241 agaggcaaat ccatgaatcg aaccagcgcc gcttcgacga gtgcagggtc gacgtcgttc
     3301 acgtcgggct cgaccggctc gggggcttcc gcgacgaagt cgacgtgctt gcgctcgtag
     3361 cgtttcagaa agtccatggc cgcattgtgc gcgacgcgaa agagccaagg ccggagcgga
     3421 ggaggctccg ttgcgttgcc gagcgcggcg tacgccctcg ccagagtctc ctgtacgatg
     3481 tcttcaccat cgaagatcga gccggtcatg cgcgcgcaat acttgaagag ctctggccgc
     3541 acctcggcca ccagatcgac gaaacgctgg tgcgcttcgt cgtcgcccgt cgcaagtagt
     3601 ggcaccagag aggcttccgt catgcaagcg gctcgatacc ggtaccggtc actccgatcg
     3661 ccgtcaaatg aggcgctagc tcaccgcgca agcccgtaac gatgccggcc gcgacctggt
     3721 cgcagtggag cggcacttcg aagcgtttca tgtacgcggc gggagagatc gacagggctc
     3781 tcgcgtagcg ctcggaggcc acggtcgcaa tcacggtgcc ttcgatgagc tgcttcggaa
     3841 cgaccgccaa aaagcgaatc ccgagcttcc tttcgtccgc gatcttctgg gcgtagccag
     3901 cgagcaacca ctgcatgcgc ttggcgccag cgtagccacc ggacaggtac gagccattga
     3961 tggccgcgcc gctcgacacc acggcaacta cactcccagg ccgtagcgga aggcgcaacg
     4021 cggcctggac gaggtggaat gcggccttgg tgtccgaggt ccacgcctct gagaacgtct
     4081 cccacgagag ctcgtcgatg gcgcccatct gcggtcgtac gcctgcggac aacaccacgt
     4141 agtctggctg gagctcagcc aagaggcgct gggcagtcga aggctcggcg gcgtcggcct
     4201 gccgaacgtc gattggcgag cccagctcgg attgtagcgc tcgtagcccc tccgccgcgc
     4261 gcgcaaccgc tgtcacgcga gctccctggg acagcagtga ctccgtcacg gccctgccca
     4321 cgccagtgct tgccccaatg acgattgctt ttttgccttg taggttcatc gctcacgctc
     4381 cttcttcgat gaagacgaat gagcgagagc aaaggatgcg ctcccgtgcg ctttcgtgct
     4441 cttttcgcgc cgcgtcaccg aggtcccctc ggcggtgctg cggcagagta ggcgtagggc
     4501 ctacttgcga aacagcgtcg cgcggtagtg ggcgagctcg gcgaccgatt gtttcacgtc
     4561 gaagagggca tcgtgctcgg cgtcggtcgg cttcgcgaac atggcggcat cgccgtacca
     4621 gagctttgcg agcatcttga tggaggagac gtcgacgctc cgatagctga gatagccgga
     4681 caggcccggc atgtagcgat cgatgaagcg cttgtcttgg ccgatggtgt tgccgcagag
     4741 cagggcaggg taggtgcacc agccggcgat gcgatcgagc aactgctttt ccgcggcgaa
     4801 gagatccgtt cgcgacgcgg cgacgcgggc gagcaggccg ttcttttcgt gcatgtcgcg
     4861 cacgtaaggg gtcatgtgct cgagtgcgct cgcgggctgc cagacatcac acgcgaactc
     4921 ctcgagaggc tcgagcgcgg cactggtgat gacgatcgcc gcttgcaaga tgacgtcgcg
     4981 cacagggtcg aggccggtca tctcgaggtc gagccagacg aggttggtgg ccgactgctg
     5041 acgcttcacg aaaggtggca tgtcactctt cctcgtcgtt gctggcggga ccgcccttgc
     5101 gggcaggttg ggatttgttc gctgaggtgg cctggttggc cgccttggct ttgctgcccg
     5161 gcttgtcctt gggcagctga atcttgggat cctttttccg gcgtcgatag acgagcagcg
     5221 tgtgcccgat gacctgcgcg actgc
//
LOCUS       DASTKS010000562         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1205063, whole genome shotgun sequence.
ACCESSION   DASTKS010000562 DASTKS010000000
VERSION     DASTKS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205063"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..905)
                     /locus_tag="VFQ35_11580"
     CDS             complement(135..905)
                     /locus_tag="VFQ35_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9931325.1"
                     /translation="MPRTVERLLPPALTFVIAMLAWEAGVRLLSVPAFLVPAPSSVLQ
                     SSWVERATLFGALVTTAEGAVLGFVLSLVFGTLAGLLLALSPRLERGLYPYTIFLQTV
                     PIVAIAPLLVLWFGAGLRAVSVSAFIVSVFPVVANSLSGLKSVDPRLYDMFRLYGANT
                     WDILVKLRLPSAIPSMTTGWRVASGLSVIGAIVGEFVAGFSEGSAGLGITILAAYRQL
                     RTDLLFGALLLSSLLGLALFSLVSLTARLLLRRLRAPG"
     gene            complement(889..1638)
                     /locus_tag="VFQ35_11585"
     CDS             complement(889..1638)
                     /locus_tag="VFQ35_11585"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931326.1"
                     /translation="MSHEFGATTPVLSGIDLEVVAGSFVALVGPSGSGKSTLLRLIAG
                     LEAPTRGRVEVEPTAAPQPGRASTAFVFQDAHLLPWRTVLDNVALPLELLGIDKAEAR
                     RRAQAPLADVELTDALERFPDQLSGGMRMRVSIARALVTEPELLLLDEPFAALDELSR
                     QRLEERLRKLWSKRGMTVLFVTHSLAEAVFLAERALVFSRRPARVVLDQHIELGAERT
                     AALRNDVAFVKQTALLYEALERNGDLDAADG"
     gene            1761..2243
                     /locus_tag="VFQ35_11590"
     CDS             1761..2243
                     /locus_tag="VFQ35_11590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9931327.1"
                     /translation="MRLVSSLAIGLLLLSACSKHKHAKHPKEAEPEAPLSEGSRCIDR
                     ARTPRKPPIDAPERMDLGQILVRHAGVRDSKGATRTREEACLRAEEAREKLLAGGDWD
                     SVFGEYSDAQGASQGVLYDVTQGSLEPAFADAAFSLKVDELSYVVETKRGFHIIWRKK
                     "
     gene            2359..>3106
                     /locus_tag="VFQ35_11595"
     CDS             2359..>3106
                     /locus_tag="VFQ35_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931328.1"
                     /translation="MRLRSLLRVLVLLVFALCRRAYAETLQAPIGGKGIALGTARVAC
                     GAPEGGWTLDSTGRLLRPPTQDDAVGKVVELKIAETVAGCRESTTVLRLVATGAWPTF
                     DPNSVVLSADQNTVEVRGRRLRGVGLAWRSDTASGIDICSAPKLDPNAERCQWAVGRG
                     LSADPNAGALQWVPAGARVGPDIVTFDSDGNRADPNTFVLTPARVVVNALLPPDASID
                     LSTGRGEVPLVHPEAIDTADCNPVHCEVHGG"
BASE COUNT          564 a          992 c         1077 g          473 t
ORIGIN      
        1 tggcgtcgat cgcagccggc tccccggagc tcgccttctt ggaacaaccg gccgcgacga
       61 gcagctgggt gagcagcgcg ccgagcgcga gtgcggacaa ccgacgtcgc gaaagttcga
      121 acacgagcgc ggttttagcc cggagcgcgc agtcgtcgca agagcaggcg cgcggtgagg
      181 ctcacgagcg aaaacagcgc gagcccgagt aacgatgaca gcaacagcgc accgaatagg
      241 agatccgtcc gaagttgccg gtaggccgcg aggatcgtga tgccgagtcc cgcgctgccc
      301 tccgaaaatc ctgcgacgaa ctcgccgacg atggcgccga tgaccgacag cccagaagcc
      361 actcgccagc cggtggtcat gctggggatc gccgaaggca aacgcaactt cacgaggatg
      421 tcccaggtgt ttgcgccata cagacgaaac atgtcgtaca ggcgcgggtc gacgctcttc
      481 aaccccgaca ggctgttcgc gacgacgggg aacaccgaca cgatgaaggc cgacaccgac
      541 accgctcgca gccctgcgcc gaaccagagc acgagcagcg gcgcaattgc gacgatcggg
      601 accgtctgca agaagattgt ataagggtag agtcctcgtt cgagccgcgg cgacagcgcg
      661 aggagaagcc cggcgagcgt gccgaagacg aggctcagca cgaaaccgag gacggcgcct
      721 tccgcagtgg tgacgagcgc gccgaagagc gtcgcgcgct cgacccaaga gctctgtaac
      781 accgagctcg gcgccggcac cagaaaggct ggaaccgaaa gcagacgcac gcccgcttcc
      841 cacgcgagca tcgcgatgac gaaggtgagc gctggtggca gcaggcgttc aaccgtccgc
      901 ggcatccaag tctccgttgc gctccagcgc ctcgtacaac agcgccgttt gcttgacgaa
      961 cgcgacgtcg ttgcgcagcg ccgcggtgcg ctcggcgccg agctcgatgt gttgatcgag
     1021 cacgacgcgg gcgggcctac gtgaaaacac gagcgctcgc tctgccaaga atacggcttc
     1081 ggcgagcgag tgcgtgacga agagcaccgt catgccacgc ttcgaccaga gcttgcgtag
     1141 tcgctcctcg aggcgttggc ggctgagctc gtcgagcgcc gcgaacggct cgtcgagcag
     1201 caaaagctcc ggctcggtga cgagcgccct cgcgatggac acgcgcatcc gcatgcctcc
     1261 ggagagctga tcggggaacc gctcgagcgc gtcggtgagc tcgacgtcgg cgaggggggc
     1321 ctgggctcgt cgtcgtgctt cggctttgtc gatgcccaag agctcgagcg gcagcgcgac
     1381 gttgtcgagc acggttcgcc acggcaagag atgcgcgtct tggaatacga acgcggtgct
     1441 cgcgcgcccg ggttggggcg ccgcggtcgg ttcgacctcg actcggcccc gtgtcggcgc
     1501 ctcgagcccc gcgatcaagc gaaggagcgt cgactttccg gagcccgagg gcccgaccaa
     1561 ggccacgaag gagccggcga cgacttcgag atcgataccg gagagtaccg gcgtggtcgc
     1621 gccgaattcg tgactcacgg agtggatcag gatgcggctg gcgatgggtc tctcctcgcg
     1681 cgtcgttcat aacacggcgg ggacaccccg tagttcgcgg cgatggtgcc cgcgggccga
     1741 gtcgtctata caagtggctc atgcggctcg tttcgagttt ggcaatcggt cttctcctcc
     1801 tttcagcctg cagcaaacac aagcacgcga agcaccccaa ggaggcggaa cccgaagcgc
     1861 cgctctcgga ggggtcgcgc tgcatcgacc gcgcccgcac cccacggaag ccccccatcg
     1921 acgctccaga gcggatggac ctcgggcaga ttctggtgcg acacgccggc gtacgtgatt
     1981 cgaagggcgc tacgcgcacg cgcgaggagg cctgcctccg cgccgaagag gcgcgcgaaa
     2041 aactgctcgc gggcggcgat tgggacagcg tcttcggcga gtactccgac gcgcaagggg
     2101 cgtcgcaggg cgtgctctac gacgtgacgc aggggagcct cgagccagcg ttcgccgacg
     2161 cggcgttctc tctcaaggtg gatgagctca gctacgtcgt ggagaccaag cgcggctttc
     2221 acatcatttg gcggaagaag tagccaccct ccgacgtttg cggaaaagtc cgacaggatg
     2281 ccgcgagccg gaccgcggag cggcgttttt gtactcgctt tgaagctcga gaagcggatg
     2341 ctatcaacgt gctcgcgcat gcgccttcgc tcattgcttc gggtgctggt cttgctggtc
     2401 ttcgcgcttt gtcgacgcgc gtacgcggag acgttgcagg cgcccatcgg tggcaaaggg
     2461 attgcgctcg gtaccgctcg cgtcgcctgt ggggcacccg agggcggctg gaccttggac
     2521 tcgaccgggc gtttgctgcg gccgccgacg caagacgacg ccgtgggcaa ggtcgtcgag
     2581 ctgaagatcg cggagacggt cgctgggtgc cgcgagagca cgaccgtcct tcgtttggtt
     2641 gcgaccggcg cctggcccac cttcgacccg aacagcgtcg ttttgtcggc cgatcagaac
     2701 acggtcgagg tgagagggcg gaggttacgc ggcgtgggcc tcgcatggcg cagcgacacg
     2761 gcgagcggca tcgacatctg ctcggcgccc aagctcgatc cgaacgcgga gcgctgtcag
     2821 tgggcagtgg ggcgcggcct gtccgccgat ccgaacgcag gcgctctgca gtgggtcccg
     2881 gccggggctc gcgtcgggcc cgacatcgtg acgttcgaca gcgatggtaa ccgcgccgat
     2941 ccgaacacgt tcgtgctcac gccggcgcgg gtagtggtga atgcgctgct tccgccggac
     3001 gcgtcgatag acttgtcgac gggccgcggc gaggtgccgc tcgttcaccc cgaggcaatc
     3061 gacacggcgg attgcaaccc cgttcactgc gaggttcacg gcggca
//
LOCUS       DASTKS010000563         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1875630, whole genome shotgun sequence.
ACCESSION   DASTKS010000563 DASTKS010000000
VERSION     DASTKS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875630"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFQ35_11600"
     CDS             complement(<1..474)
                     /locus_tag="VFQ35_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9931329.1"
                     /translation="MPEPLEDHPGRILSGRYELVEPLGKGGMAVVWRGVMHGADGFRR
                     AVAIKRIDPSCRGFLEVLEMFVEEARVGGLLTHSNIVQVYDFGADENGERYLVTELVR
                     GPHFGQWLAAHQALGNPAPWELVAAISVEVLRALDAAHSHVDASGRSLAVLHRDVT"
     gene            777..2000
                     /locus_tag="VFQ35_11605"
     CDS             777..2000
                     /locus_tag="VFQ35_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HET9931330.1"
                     /translation="MLGASSVDRVRFRLATGLLLTAWLAACSGTAKNDLFQSPISNAG
                     NESQAGGASSASGGAQPAASGGSTASGGELGNAGAGAEAFGGDENAGGTAPLGFGGET
                     SFGGMAVDSAGGASAGALNQGGTVNEAGASASGGGATTGGTDSGGVANGGATAGGGSS
                     ATGGISGDGNANGGASGGGNTNGGGLGDGGNSASGGSGASGGGGSENGGVPNGGGANG
                     GASSERTGGSSCQRSTEICDGVDNDCNSQIDENACPPKCTGFVAAGRHFMACSVVVAQ
                     ADAASLCATHGMRLAWLDSLDKVNAVLASLQEQNHRGMTPVFDEVYIGATDAEQEGHW
                     HWAGGADFWLGNGSGSAVNGAFSNWSSGRPNNFPKMPGENCAVSVIEFPSDGNPGQWN
                     DVTCTEPHGALCEVP"
     gene            complement(2083..3357)
                     /locus_tag="VFQ35_11610"
     CDS             complement(2083..3357)
                     /locus_tag="VFQ35_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9931331.1"
                     /translation="MTHAPSTSSPRGLGLRLSILFFIDVAVAAAYYPFLSLHLAKTLH
                     LTASEVALVYAMGPLTAFVGPPLVGYLADRVLPAERSLALIGVFRTLVLLLASRAHDF
                     GGILFAMALVGLTMAPAGVLSFTVAFHHLHDSRSIGKSRVFGTVSWISALWATSAYMH
                     HLGGVEQQLAHTRVMFVFAAAVSALGAVYALTLPHTPPAKAQGAFAFVEALSLLSNRS
                     FRALIAASALAAACLQFHFMLWPLFYTDKVTGLGMDLSRASSLSSIAQLLELGLFPAL
                     GLLIRRFGIRRVLLVGLLAWPIRFAAYAAGKPAFFVVGIQILHGVNVVCAAMVSQIAV
                     DRVAPRHARASSHALLFVATLGAGNLLGQLTCGALLGACTLGADGHDWPTIFSLPLAL
                     GALATLLVFLGYREDTAAEPTLALEQTKSQSA"
     gene            complement(3450..4187)
                     /locus_tag="VFQ35_11615"
     CDS             complement(3450..4187)
                     /locus_tag="VFQ35_11615"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9931332.1"
                     /translation="MRRNNEAASLYRSWESIVESVAVPNGERKRPPTSGAAPKRPASP
                     LRVLPGGAERTLSDEALVDAVQRGDQRVAQHLYDRLYPVVDRTLYRIFGKREADHDDL
                     MQAAFEQIVVTLGRRTYAGDCSLKTWAASITSHVAFNALRSRRRERRLLDRGIELDGD
                     NLAASTFGEDSASARLELNRVQQHLLAMKPEQAEALFLCDVLDHKLAEVARLTHVSVA
                     AATSRLVRGRRELHRRLAAERKAERFR"
BASE COUNT          757 a         1274 c         1525 g          661 t
ORIGIN      
        1 cgtcacgtcg cggtgcaaca cggcgaggct ccggccactg gcgtcgacgt gcgagtgggc
       61 cgcgtcgagg gcgcgcagca cctctaccga gatcgcggca acgagctccc acggcgcagg
      121 gttgccgagg gcttgatgcg cagcgagcca ttggccgaag tgcggtccgc gcacgagctc
      181 cgtgacgagg tagcgctcgc cgttctcgtc ggcgccaaaa tcgtacacct gcacgatgtt
      241 cgaatgcgtg agcagcccgc ctacgcgcgc ctcttcgacg aacatctcga gcacctcgag
      301 gaagcctcgg cacgacggat cgatgcgctt gatcgcgacc gcgcgccgaa agccgtctgc
      361 gccgtgcatc acgccacgcc aaaccaccgc catcccgccc ttgccgagcg gttccaccaa
      421 ttcgtagcgg cctgagagaa tgcgacccgg atgatcttcg agcggttcgg gcatgagcgg
      481 ctaggtccga ggaattcttt cgatgaagcg aaggacggct acagtctacc gagtgagtca
      541 gactcgtacc aatgcaccgg tggtgggtca gtcgttcggc gtctgaaatc agcggctttt
      601 atgctgagtg agaacggcac ggcgctcccg agggcgagca tccgtgaacc attttctgcg
      661 gaagcgccgc ggttgccgcg agcgcgcgtg gtgcgacaac acccgccgga gtaggctcga
      721 aagctctctt cttcgctgtt gattgcgtgg agcgacgcgc tcaaactcgc gagtgaatgc
      781 tcggcgcatc ttcggtcgac cgcgttcgct ttcgactcgc gaccggtttg ctgctcaccg
      841 cgtggcttgc ggcgtgtagc ggcacggcca aaaacgactt gtttcagtct ccaatcagca
      901 acgcggggaa cgaaagtcaa gctggcggcg cgagctcggc gtcgggtggg gcgcagccgg
      961 cggcgtccgg gggtagcact gcgtccggcg gtgagctggg caacgcgggc gccggtgcag
     1021 aggcgttcgg cggcgatgag aacgcgggcg gaaccgcgcc gcttggtttc gggggagaga
     1081 cgagtttcgg tgggatggcg gtggactcgg cgggtggcgc gagtgcggga gcgctcaacc
     1141 agggcggcac cgtgaacgaa gcaggagcgt cggcatccgg tggcggagcg acgacgggcg
     1201 gcactgattc gggcggcgtg gcgaacggcg gcgcgactgc cggtggaggc tcgtcggcga
     1261 cgggcggcat ctcgggtgat ggcaatgcga atggcggcgc ttcgggtggt gggaacacca
     1321 acggcggtgg gctcggtgac ggcggcaact cggcgagcgg tggctcgggt gcgagcggcg
     1381 gtggagggag cgaaaacggt ggcgtgccca acggcggcgg tgcgaacggc ggtgcctcga
     1441 gtgagcgcac cggtggctcg agctgtcaga ggtcgaccga gatctgcgac ggcgtcgaca
     1501 acgactgcaa ctctcagatc gacgagaacg cctgcccccc gaagtgtacg ggcttcgtcg
     1561 ccgcgggccg tcatttcatg gcttgttcgg tggtggtggc tcaggcggac gcggcgtctt
     1621 tgtgtgcgac ccacggcatg cggctcgctt ggctcgattc gctggacaag gtgaacgccg
     1681 tgttggcgag cttgcaggag cagaaccatc ggggcatgac gccggtgttc gacgaggtgt
     1741 acatcggcgc gaccgatgcc gaacaggagg ggcactggca ctgggccggc ggcgccgact
     1801 tctggctcgg taacgggagc ggaagcgccg tgaacggcgc gttcagcaac tggtcgagcg
     1861 ggcgccccaa caacttcccc aagatgccgg gagagaattg cgcggtgagc gtcatcgagt
     1921 ttccttccga tgggaaccca ggtcagtgga acgacgtcac gtgtaccgag ccgcacggcg
     1981 ccctctgcga ggtgccgtaa ggcgcgtgac tcgcgggaac gcccgcggcg cgcgaatgga
     2041 tcagtcagtc gcctcggtcc gggatctaga actggctcta aactaagcgc tctgcgactt
     2101 ggtttgctcg agcgcgagcg tcggttcggc ggcggtatct tcgcggtacc ccaagaacac
     2161 gaggagcgta gcgagcgccc cgagcgcgag cgggaggctg aagatcgttg gccagtcgtg
     2221 gccgtcggcg cccaacgtgc aggcgccgag gagcgcgccg caggtgagct ggccgagcag
     2281 gttgcccgca cccaaggtgg ccacgaacaa cagcgcgtgc gagctcgctc gagcgtgacg
     2341 cggcgccact cggtcgaccg cgatctggga gaccatcgcg gcgcagacca cgttgacgcc
     2401 gtgcaaaatc tgaatgccca ccacgaagaa cgcgggtttg ccggcggcgt aggctgcgaa
     2461 acggatgggc caagcgagca acccaaccaa caggactcgc cggatcccga agcgtcgaat
     2521 cagcaggccg agcgctggaa ataagccgag ctcgagcagc tgcgcgatcg aagacagcga
     2581 actcgcgcgc gacaggtcca tgccgagccc cgtcaccttg tccgtataga agaggggcca
     2641 cagcatgaag tggaactgca ggcacgccgc ggcgagcgcc gaggccgcga tgagtgcgcg
     2701 gaagctccgg ttcgaaagca aagacagcgc ttcgacgaac gcgaaagcgc cttgtgcctt
     2761 tgccggcggc gtgtggggca gggtgagcgc gtacacggcc ccaagcgccg agaccgcggc
     2821 tgcgaacacg aacatgaccc gagtgtgagc gagctgctgc tcgactccgc ccaagtgatg
     2881 catgtaggcg ctcgtggccc acaaggccga gatccaactc accgtaccga agacgcggct
     2941 ctttccgatg ctgcgggagt cgtggaggtg atggaacgcg acggtgaaac tcaggacgcc
     3001 cgcgggtgcc atggtgagcc cgacgagggc catcgcgaac agaatgccgc caaagtcgtg
     3061 ggcgcgactc gcgagcagga gcacgagcgt ccggaacacg ccgatgagag cgagggagcg
     3121 ctcggcgggc aatacgcgat cggcgagata gccgacgagc ggtgggccga cgaatgcggt
     3181 gagcgggccc atcgcataga cgagagcgac ctcactggcg gtaaggtgca gtgtcttcgc
     3241 gaggtgcagc gaaagaaacg ggtagtaggc ggcggcgacc gcgacgtcga tgaagaaaag
     3301 tatcgagagc cggaggccga gcccgcgggg actcgaggtg gaaggcgcgt gcgtcaagtg
     3361 tcggcggggt ttggcacagc ttgccgttcc cgcaccccca aacggcttag catcgtcgct
     3421 gctaaggcgc ggagggaacc gtcagtcctt caacggaagc gctcggcttt tcgttcggcg
     3481 gcgagccggc gatgcagttc tcggcgtccg cgtaccaggc gggatgttgc ggcggcgact
     3541 gaaacgtgag tcagccgcgc tacctccgcg agcttgtggt cgagcacgtc gcagaggaac
     3601 agcgcttctg cctgctccgg cttcatcgcc aagaggtgct gctgcactcg gtttagctcg
     3661 agccgcgccg aggcgctgtc ttccccgaag gtcgacgcag ctaggttgtc gccgtcgagc
     3721 tcgatgccgc gatcgagcag gcggcgctct cgccttcgcg agcgcagcgc gttgaaggca
     3781 acgtggctcg tgatgctggc agcccaggtc ttgaggctgc agtcgccagc gtaagtcctt
     3841 ctgcccaatg tgaccacgat ctgctcgaac gctgcttgca tcagatcgtc atggtccgct
     3901 tcgcgcttgc cgaagattcg atagagcgtt cgatcgacca ccgggtacag gcgatcgtag
     3961 aggtgctgcg cgacgcgctg atcgccccgc tgaacggcgt cgacgagcgc ctcgtcggac
     4021 agcgtgcgct ctgccccgcc cggcaagacg cgaagagggc tcgccggcct tttcggcgcc
     4081 gcgcccgagg tgggaggtcg cttgcgctcg ccgttcggta cggccaccga ttcgacgatg
     4141 ctctcccagc ttcgatacag actcgcggct tcgttattgc ggcgcacggg ctaaccaggg
     4201 ctgtaggttt aggtccg
//
LOCUS       DASTKS010000564         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_981822, whole genome shotgun sequence.
ACCESSION   DASTKS010000564 DASTKS010000000
VERSION     DASTKS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981822"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFQ35_11620"
     CDS             complement(<1..246)
                     /locus_tag="VFQ35_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET9931333.1"
                     /translation="MLFNTLQYAKFFAAVFVASWLLARAPRVRIGFLLAASYYFYAAF
                     DYRFLPLIFISSTADFWLARRIGETREPRERKLLLWLT"
     gene            311..1165
                     /locus_tag="VFQ35_11625"
     CDS             311..1165
                     /locus_tag="VFQ35_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9931334.1"
                     /translation="MTAQPPFFRLLSSSVGALSSVSPARFAFWASSSGLSALAANAVL
                     GGRPGAFVLSAASLGWGALVTSGVFFPWLEMYGGVVCRGPRGRAEVALTFDDGPHPDT
                     TAQVLRVLEGTPHRATFFVLGQKVRKYPELARAIVDGGHTIAIHGDTHDRLHSFRTPW
                     RVRDELVRARDSIEAASGKRAELFRPPLGHTTPATLRGARSAGVTPIGWSARGYDGIR
                     GRQPEAVVRGVTRSLDDGAIVLLHDAAENDDFVPAGVVALPALLDELARRKLESVALE
                     RWFPDAAR"
     gene            complement(1252..2793)
                     /locus_tag="VFQ35_11630"
     CDS             complement(1252..2793)
                     /locus_tag="VFQ35_11630"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9931335.1"
                     /translation="MRLGIDFGTTRTVVAAAIEGRYPVAAFDTGRGFVDFVPGLAVGL
                     QSGMALGWDAVERLGADAPSALRSIKRVVSGLSPDDTVEVLSGSPTALELTTDYLVGL
                     RRMIVERSNLDVDPAEPLEAMVAVPAGASTRQRFLTMEAFTRAGFRVSGMLNEPTAAA
                     IEYAHRSLGTLSRRSPKRYVVVYDLGGGTFDTSAVSLAGRRYELMATEGISELGGDDF
                     DQAILDLALESLGNDGAQLNSVALARALELCREAKEGLTPSTRRMLIDLGTVVDGMPS
                     VTLETSKVYDRVEPLVRRTLSMIDRVFTQLASHGIDTNNPRELGAVYLVGGATAFPLV
                     GRLLRETFGRKVLLATEPHAATAVGLAIANDPEADIFVREAVTRHFGVWREGDSGRDK
                     VFDPIFAKGTLSDTGEHVVERHYSPVHAVGHLRFLECSELGDHGQPAGDLTPWCDLFV
                     PYVPELAERPDLGSLSGGRDLREGSEHVVERYAYERDGKVRVAIENVTRGFRREFVLG
                     APSAR"
     gene            3413..>4473
                     /locus_tag="VFQ35_11635"
     CDS             3413..>4473
                     /locus_tag="VFQ35_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9931336.1"
                     /translation="MADAFGLRLALTLAALFLFAHCSVDRPYSSRSSPTGARGGDAGT
                     KPNGQGGSANSPSGTGSAPSISAEVDDPCQEEGLRTCLGSGQKQRLVCSNGVYERDTD
                     CTESENCDELSGACSPIVRECAGKSAGYRFCDAQGKLKVCGTDLVRTETEECNGTCRD
                     GACKPLGCGDGVVTPPEDCDDGNVVDDDGCTSQCKSARCGDAVQQAGEDCDDGNPADN
                     DSCVKCRFARCGDGVVGPNEDCDDGNAVDNDACSNACKFSKCGDGIQHSGEDCDDGNA
                     VDNDTCSNACHRPKCGDKIVQAGEACDDGNTVDNDTCSNRCTVPGCGDSVTQTATEEC
                     DDGNAVNTDGCTVACKKPK"
BASE COUNT          794 a         1454 c         1518 g          707 t
ORIGIN      
        1 ggtgagccac aaaagcagct ttcgctcgcg aggttcgcga gtctcaccga tgcgccgcgc
       61 gagccagaag tccgccgtcg acgagatgaa aatgagcggc aagaaccggt aatcgaaggc
      121 cgcgtagaag tagtagctcg ccgcgagcag aaagccgatg cgaacgcgtg gcgctcgcgc
      181 caagagccac gacgccacga agacggccgc gaagaacttg gcgtattgaa gcgtgttgaa
      241 cagcacggcg gacggcgcgc ctgccggccg actaccgggg agcgcggccg atggtagtat
      301 ctcggctcgt atgaccgcgc agcccccttt ctttcgtctg ctgtcgagca gcgtgggcgc
      361 gctctcatcc gttagcccgg cgcgattcgc gttttgggca tcttccagcg ggctttcggc
      421 gctggccgcg aatgcggtgc tcggtgggcg cccgggcgcg ttcgtgctct cggcggcctc
      481 gctcgggtgg ggagcgctgg tgacgagcgg cgtgtttttt ccctggctcg aaatgtatgg
      541 tggcgtggtg tgtcgcggcc cacgcggtcg ggccgaggtc gcgctgacgt tcgatgacgg
      601 cccgcatcca gacaccactg cccaggtgct gcgcgtgctc gaaggcacgc cgcaccgcgc
      661 gacgttcttc gtgctcggac aaaaggttcg aaagtacccc gagctcgcgc gcgcgatcgt
      721 cgacgggggg cacaccatcg cgattcacgg cgacacgcac gaccgcctgc actcatttcg
      781 gacgccctgg cgcgtccgcg acgagctcgt gcgcgcgcgg gactcgatcg aagccgcttc
      841 gggtaaacgc gccgagcttt tccggccgcc gctcggacac acgacgccgg ccacgttgcg
      901 cggcgcgcgc agcgcgggtg tgacgccaat cggctggtcc gcgcgcggct acgacggcat
      961 tcgcggacgc cagcccgaag cggtggtacg cggtgtgacg cgatcgctag acgacggcgc
     1021 gatcgtcctt ttgcacgacg ccgccgagaa cgacgacttc gttcccgccg gagtcgtcgc
     1081 gttgcccgcg ctcctcgacg agctcgcgcg gcgcaagctc gaaagcgtcg cactcgagcg
     1141 ctggtttccc gacgcggcac gctagcctag ctagccaggt gtcggcatag tgccaaagct
     1201 cgtcgggccg acgcagcggc gcgcgctttg gcccgcatct tccaagaagc gttagcgcgc
     1261 gctcggcgcg ccgagcacga attcgcgacg gaagccgcgg gtcacgttct cgattgcgac
     1321 ccgcaccttg ccgtcgcgct cgtaggcgta gcgctcgacg acgtgctccg acccttctcg
     1381 caggtcgcgc ccgcccgaga gactgccgag atcgggccgc tcggcgagct ccggcacgta
     1441 cggcacgaac aagtcgcacc acggcgtgag atcgcccgct ggttgaccat ggtcgccgag
     1501 ctcactgcac tcgaggaagc gcaggtgccc caccgcgtgc accggtgagt agtgtcgctc
     1561 gacgacgtgc tcgccggtgt ccgacagcgt gcctttggcg aagatggggt cgaacacctt
     1621 gtcgcgaccg ctgtcgcctt cgcgccatac gccgaagtgg cgcgtcaccg cttcgcgcac
     1681 gaagatgtcg gcttcggggt cgttcgcgat cgcgaggccc accgccgtgg ccgcgtgcgg
     1741 ctcggtcgcg agcagaacct tgcggccgaa cgtctcgcgc aagagccgtc caacgagcgg
     1801 aaacgcggta gcgccgccga cgaggtacac ggcgccgagc tcgcgcggat tgttcgtgtc
     1861 gatgccgtgg ctcgcgagct gagtaaacac gcggtcgatc atcgagaggg tgcggcgcac
     1921 gagcggctcg actcgatcgt aaaccttgct ggtttcgagc gtgacgctgg gcatgccgtc
     1981 gacgacggtg ccgagatcga tgagcatgcg tcgggtgctc ggggtgagcc cttctttcgc
     2041 ttcgcggcaa agctcgaggg cgcgtgccag cgcgaccgag ttcagctgag cgccgtcatt
     2101 gccgaggctc tccagcgcca aatccaagat ggcctgatcg aagtcgtcgc caccgagctc
     2161 ggagatcccc tccgtcgcca tcagctcgta acggcggccc gcgagcgaaa cggcggacgt
     2221 gtcgaacgtt cccccaccta aatcataaac gacgacgtaa cgttttgggc tccgccgcga
     2281 caacgtgccg agcgaacgat gggcgtactc gatagcagcg gcggtcggtt cgttgagcat
     2341 gccgctcacg cggaaacccg cgcgcgtgaa cgcttccatc gtgaggaagc gctggcgcgt
     2401 gctcgcgccg gccggaacgg cgaccatcgc ctcgagcggc tccgctggat cgacgtccag
     2461 gttgctgcgc tcgacgatca ttcggcggag gccgacgagg tagtcggtgg tgagctcgag
     2521 cgcggtcggc gagcccgaga ggacctcgac cgtgtcgtcc ggagaaaggc cgctcaccac
     2581 tcgcttgatg gagcgcaacg ccgaaggcgc gtccgcgccc agccgttcga ccgcgtccca
     2641 gccgagcgcc attccggact ggagcccgac cgccagcccc ggtacgaagt ctacgaagcc
     2701 gcgcccagtg tcgaatgccg ctaccggata gcgcccctcg atcgccgcgg cgacgaccgt
     2761 ccgtgtcgtt ccaaagtcga ttccgagtcg catcgagccc gtggcggcat cttgccggta
     2821 tgtgagggcg aggccaagcg ccgagatgag cggacctgac gactttgaaa cggcgcggca
     2881 acgaatcccc gcgagtcggc ggaattccgc gcaattatgc cccgagggga aacctccggt
     2941 cgaggagctg cggccttttt cgggtcgggc gccgtgccac cggagagcta cgggtgtcgc
     3001 gagttctccg ccccgttgcg agtcgagatc ctgcggctgc gaagtgcgag ctcaccgcgc
     3061 tggacgccga ccgacgtttt cgtacgcgcc gccgacccga tttgcgacag ggcgtcgagc
     3121 gaggcttgct aacgaagtca cgctcgaggg ctcattgcac ggccggaacg acggacgaaa
     3181 cgtctcaggg gagtgatggg cccgcggcgc ggcacgcggg gttcgacgta gcgctggtag
     3241 atcactccgc tttgtacctt cttgcgttga acgcacgcgt cgcggaccgg tttggtcgca
     3301 cgagcataca agatgaactc tgacgcgagc gtacgctccg cctcgtgaac cggatgcacg
     3361 aaggcatgag cgtcgaatga ctcgcgcgcg aagccttttc agaagtagcg cagtggctga
     3421 cgccttcgga ttgcgactcg cgctaacgct ggcggcgcta ttcctgttcg cgcattgctc
     3481 ggtggacagg ccgtattcga gtagatcgtc gccgacggga gcgcgtggcg gcgatgccgg
     3541 aaccaaaccc aatgggcaag gcgggagcgc caacagtccg tcgggcactg gcagcgctcc
     3601 ttcgatttcg gctgaggtcg acgacccgtg ccaagaggag ggcctgcgca cgtgcctagg
     3661 ctcagggcaa aagcaacggc tggtctgctc gaacggggtc tacgagcgag atacggactg
     3721 caccgagagc gaaaactgcg acgagctctc cggcgcttgc tcgcccatcg tgcgcgagtg
     3781 cgccgggaag tctgccggtt atcggttctg cgacgcgcaa ggcaagctca aggtgtgcgg
     3841 gaccgatctc gtgcgcaccg agaccgaaga atgcaacggc acctgtcgag acggagcgtg
     3901 caaaccgctg ggctgcggtg acggcgtcgt tacccctccc gaagactgcg acgacggcaa
     3961 cgtcgtcgac gatgacgggt gcacgagcca atgcaagagc gctcgctgcg gagacgcggt
     4021 gcagcaagca ggcgaagact gcgacgacgg caaccccgcc gacaacgact cctgcgtaaa
     4081 atgccgcttc gcacgctgtg gagacggcgt cgtcggtccg aacgaagact gcgacgacgg
     4141 taacgccgtc gacaacgatg cctgctccaa cgcttgcaag ttctcgaagt gcggagacgg
     4201 cattcagcat tcaggcgaag actgcgacga cggtaacgcc gtcgacaacg atacctgctc
     4261 caacgcttgt caccgcccga agtgcggaga caagatcgtt caggcgggtg aagcctgtga
     4321 tgacgggaac accgtcgaca acgacacctg ctccaatcgt tgcacggtcc cgggctgcgg
     4381 cgactcggtc acccagaccg ccaccgaaga gtgcgacgac ggcaacgcgg taaacaccga
     4441 cggctgcacg gtcgcctgca aaaagccgaa gtg
//
LOCUS       DASTKS010000565         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1651854, whole genome shotgun sequence.
ACCESSION   DASTKS010000565 DASTKS010000000
VERSION     DASTKS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651854"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFQ35_11640"
     CDS             complement(<1..429)
                     /locus_tag="VFQ35_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdH-redoxin"
                     /protein_id="HET9931337.1"
                     /translation="MLRRPHRLVLCALLLAVGCHTRTHSDAENNAAPKTNELPALSIK
                     ADTPNLLLTWIDDKGDFHVVDKPADVPAEARGAVRVVATDKEAGTSDQVYVADLNQTA
                     PDGSYKVKTLSRGAWDELGASKRKSRLEALAPASAAPSSSA"
     gene            complement(524..2455)
                     /locus_tag="VFQ35_11645"
     CDS             complement(524..2455)
                     /locus_tag="VFQ35_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9931338.1"
                     /translation="MALLEIADLEFGYSNEPLFTKVTFRLEAGERAALVAPNGAGKST
                     LLRVIAGELQADKGSVLLKKPATVGYYRQSHEARSEGTVLDALMSGFSELIDVRRELS
                     LAQHAAETGAPADLDRLANALDRYHQSGADRLEQRVQSIATRLGFADRELSRLVSTLS
                     GGERGRLALGVVLATTPDLLLLDEPTNHLDLESIRWLESYLREQRAAVLVVSHDRAFL
                     DAVCPTTMELGRTTFRVYPLPYTAYHEERREDLEREQKLAEEQAAFVAKTEDFIRKNI
                     AGQKTKQAQSRRRMLDKLERVGRPEDVWARAERVRFRFAEAPRSGDIVLEAKGLAAER
                     GGNRLFAGVDLLVRRGDRIGIVGANGSGKSTLMGLLAGRGHEADEGAVRRGTNLYEGY
                     FDQHLGSLDPARTGIDEIRSVRGDLNSDGARQYLARFRFYGDDPLRKVESLSGGERTR
                     LALGKLLLEPRNLLFLDEPTNHLDIPAAEILEEALVGFEGTVILVSHDRRFLETVTTR
                     TLAFTEHGVDVYEGGFKDYTDALERQRKAALEPAPKKPKTPSTPVKPEPAAGADAYRA
                     QKAAARDLERKRKRVQELETHISEGERETQRYRSELKEVPSTDWERLHALAKKEQELT
                     RRLERWTAEWVKLSEELET"
     gene            2617..3375
                     /locus_tag="VFQ35_11650"
     CDS             2617..3375
                     /locus_tag="VFQ35_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931339.1"
                     /translation="MELGDTPESTAPKPWQVARAEAIEGILQKRGRRIGRTLDVGLAD
                     GYTGARLFERLGMSSYVGYDAALTGAECEALSREAFRITNTLPEAANDFDLVLVRDAL
                     EYAADDALLLRDVRARVHSRGRVLVLVPAFEGLFSDYDRQLGRHRRYSLEELCGHLAS
                     AGMPVQSSGYLFGSLLLPRVLRRASEVARSLVSRHRAGAPDLPSAARLGGVARRLAAS
                     ALELENSALLSLNARGVKVPGITAWALSDLYEVR"
     gene            3512..>3996
                     /locus_tag="VFQ35_11655"
     CDS             3512..>3996
                     /locus_tag="VFQ35_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9931340.1"
                     /translation="MNFKHYFPTFRTRWRFLRECLSEVGHRQRALHIGCGEGDHDRLI
                     KRFADELVACDLNQADVEHARAVNADEPGIEYRVEDATALSFADLSFDTVVCMEVIEH
                     VSDPRKLLAEIARVLKPEGRAILTCPSEAFPVTYDPINYLLAPFDKHVSFGAYGYGHD
                     WL"
BASE COUNT          609 a         1296 c         1318 g          773 t
ORIGIN      
        1 cgccgaggag ctcggggccg cgctcgctgg cgccaacgct tcgaggcgtg atttgcgttt
       61 gcttgcgcca agctcgtccc aagctccgcg cgagagtgtc ttcaccttgt agctgccgtc
      121 gggcgcggtc tggttcaggt cggcgacata gacttggtcg ctcgtacccg cttctttgtc
      181 cgtcgcgacg acgcggaccg cgccgcgtgc ctctgccggg acgtctgccg gcttgtcgac
      241 gacgtggaag tcacccttgt cgtcaatcca agtaagaagg aggttgggtg tgtccgcttt
      301 gatcgaaagg gcgggcagct cgttggtctt cggcgccgcg ttgttctcgg catccgagtg
      361 cgtgcgggtg tgacatccca ccgcgagcag cagggcgcaa aggacgaggc ggtgcggcct
      421 tcggagcatc gcgcattcta tagcacggtt ctcgcgctgc tttcggcacg ccgagggccg
      481 cgcttgcctt ggttttcgca gtgacggcgg ctctcgatcg ctatcacgtt tcgagctctt
      541 cggagagctt cacccactcg gcggtccagc gctcgagcct gcgcgtcagc tcttgctcct
      601 ttttggcaag ggcgtgcaag cgctcccagt ccgtgctggg gacctccttg agctcgctgc
      661 ggtaacgctg cgtttcgcgc tcgccttcgg agatatgggt ctcgagctcc tggacgcgtt
      721 tgcgcttgcg ctcgaggtcg cgcgcggcgg ccttttgggc gcgataggcg tcggcacccg
      781 ccgcgggctc cggcttgacg ggtgtgctgg gagtcttcgg cttcttcgga gccggttcga
      841 gcgcggcttt gcgctggcgc tcgagcgcgt cggtgtagtc cttgaacccg ccttcgtaga
      901 cgtcgacccc gtgctcggtg aacgcgagcg tccgcgtcgt gaccgtctcg aggaagcgcc
      961 ggtcgtgcga gacgagaatg acggttcctt cgaaaccgac gagggcctct tcgaggatct
     1021 cggcggccgg gatatcgagg tgattcgtcg gttcgtcgag aaacaggagg ttccgcggct
     1081 cgagcaagag cttgccgagc gcgaggcgcg tccgttcgcc gccggagagg ctctcgactt
     1141 ttcggagggg gtcgtctccg tagaagcgaa agcgggcgag gtactgtcgc gcaccgtccg
     1201 aattcaggtc gccgcgcacg gagcggatct cgtcgatgcc ggtgcgcgct gggtcgagcg
     1261 atcccaggtg ttgatcgaag tatccctcgt acaggttcgt gcctctccgt acggcgcctt
     1321 cgtccgcttc gtggccacgg cccgccagca accccatcaa cgtcgacttg cccgagccgt
     1381 tcgcccccac gatgccgatg cgatctcccc tccgcaccaa caagtcgacc cccgcgaaca
     1441 agcgattgcc gccgcgttcc gccgcgagcc ccttcgcttc gaggacgatg tccccgctgc
     1501 gtggcgcttc cgcgaaacga aagcgcaccc gctcggcgcg cgcccacacg tcttcgggcc
     1561 gtcccacgcg ctcgagcttg tccaacatgc gacggcgcga ctgcgcttgc ttggtcttct
     1621 gtcccgcgat gttcttgcgg atgaagtctt cggtcttcgc gacgaacgca gcttgctcct
     1681 ccgctagctt ctgctcgcgc tccaagtctt cgcggcgctc ctcgtgataa gcggtgtagg
     1741 ggaggggata cacccgaaaa gtggtgcgcc cgagctccat cgtcgtcggg cacaccgcgt
     1801 ccaagaaggc gcgatcgtgc gagacgacca agaccgccgc gcgctgctcg cgcaggtaac
     1861 tctcgagcca gcgaatgctc tcgagatcca gatggttcgt cggctcgtcg agcaagagca
     1921 aatcgggcgt cgtggcgagc accacgccga gggctagtcg tccgcgctcg ccacccgaga
     1981 gcgtgctgac caggcgcgaa agctcgcggt cggcgaaccc gagccgggta gcgatgctct
     2041 gcacgcgctg ctcgaggcga tcggcgcctg attgatggta gcgatcgagc gcattggcga
     2101 gtcgatcgag gtcggcaggg gcgcccgtct cggccgcgtg ttgtgcgagt gaaagctctc
     2161 gtcgaacgtc gatgagctca gagaagccgg acatgagcgc gtcgagcacc gtgccttcac
     2221 tgcgagcctc gtggctttgc cggtagtatc cgaccgtcgc cggtttcttc agcaagacgg
     2281 agcctttgtc ggcctggagc tcacccgcga tcactcgcag cagcgtgctc ttacccgctc
     2341 cgttgggcgc gaccaacgca gcgcgctcac cggcctccaa tcggaacgtc accttcgtga
     2401 acaggggttc gttcgaataa ccgaactcga ggtctgcaat ttcgaggagc gccacgggaa
     2461 ccggcgcagc ctgacacgtc agcgggcgct tgctagcccc gcgacgactg tgctggggcg
     2521 accggccgcc ctgggcgccg atgcccccaa gcatcgccga aatcaagagc gaaaccgccg
     2581 gaaaggaggt gtttcgcgtt aagctggagc ccgacgatgg agctcggtga cacgcctgaa
     2641 tccaccgcgc ccaagccatg gcaagtggct cgcgccgaag cgattgaggg cattctgcaa
     2701 aagcgggggc ggcgcatcgg gcggaccttg gacgtaggtc ttgccgacgg ctacacgggc
     2761 gcgcgcttgt tcgagcgctt gggcatgagt tcgtacgtgg gttacgacgc cgcgctaacc
     2821 ggcgccgagt gcgaggcgtt gtcgcgcgaa gccttccgca tcacgaacac cttgcccgag
     2881 gctgccaacg acttcgactt agtcttggtg cgtgatgctc tcgagtacgc cgctgacgat
     2941 gcgttactgc tccgggacgt tcgcgcgcgt gtccattcgc ggggccgcgt cctcgtcttg
     3001 gtgccggcgt tcgaagggct gttttcggac tacgatcgac agctcgggag gcatcggcgc
     3061 tactcactcg aggagctgtg cggccacctc gcgagcgcgg ggatgccggt acaaagctcg
     3121 ggctatctgt tcgggtcctt gctgttgcca cgggtcctcc ggcgggcgag cgaagtcgcg
     3181 cgttcgctcg tctcgcgaca ccgcgccggt gccccggatc ttccgtctgc ggcccgactc
     3241 ggcggagtcg cgcggcgctt ggctgcctcg gctctcgagc tcgagaacag cgcgctcttg
     3301 tcgctcaacg cgcgcggcgt gaaagtcccg gggatcacgg cctgggcgct cagcgatctc
     3361 tacgaggtcc gatgagcccg gctcgctctc tttactggat ctttccgctc gcgccggttc
     3421 tcctcggggg tcgcatcgga ccgaaatacg gtaagctccg cgcccgctcg tcctcgagct
     3481 gacaagcttc gaacgtcgct ccgccgagtc aatgaacttc aagcactact tcccaacctt
     3541 ccgaacgcgc tggcgatttc tacgagagtg tctttcggag gtcggccacc gtcagcgcgc
     3601 gttgcacatc ggttgcggcg agggcgatca cgaccggctg atcaagcgtt ttgccgacga
     3661 gctcgtcgcc tgcgatctga atcaggcgga cgtagagcac gctcgagccg tgaatgccga
     3721 cgagcccggc atcgagtatc gcgtcgagga tgcgaccgcg ctgagcttcg ccgacctgag
     3781 cttcgatacg gtcgtatgca tggaagtgat cgagcacgtg agcgatccgc gcaagctatt
     3841 ggcggagatc gcgcgcgttc tcaagcccga ggggcgcgcc attctaacgt gtcccagcga
     3901 ggcgttccca gtcacttacg atccgatcaa ctacctgctc gcgcccttcg acaagcacgt
     3961 gtccttcggt gcgtacgggt acgggcacga ttggct
//
LOCUS       DASTKS010000566         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_758878, whole genome shotgun sequence.
ACCESSION   DASTKS010000566 DASTKS010000000
VERSION     DASTKS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758878"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFQ35_11660"
     CDS             complement(<1..841)
                     /locus_tag="VFQ35_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931341.1"
                     /translation="MKPTVLIVDDDRSFRILTEAGLAGEGFEVRSAATLRKAREELDS
                     GAPDVMILDRRLPDGDGIALLQELSASGGAVIPVIVVTAYGDIDSAVAAIQAGAVDYL
                     TKPINVADLLIKIHKVLETRGLRERLALANVAHRRPIGIEPQSAALRKVYSELERLSG
                     SPQTPVLLLGPSGAGKQFAAELLHRLTYADNEGAPFVDVNCAALPEQLVESELFGHER
                     GAFTDAKAARRGLIEMANGGTLFLDEVTELPEASQAKLLKFLDSARFRRVGGQREIEV
                     ELRV"
     gene            complement(838..2517)
                     /locus_tag="VFQ35_11665"
     CDS             complement(838..2517)
                     /locus_tag="VFQ35_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9931342.1"
                     /translation="MNRVNREAKLDNNSQNAAVTLAAAHARLAQWALAVYVGTAVVVA
                     SLLIAVLVTEEAHDEEQLENSMRMAAIDRSYALRRYLDLLTTELGRLASRAEIQAQDN
                     DSSPERELLEIAHKRSTLFDVGVAILDLEGAVVWAEPHAFLPQGTSFKSAPWFPDISR
                     SRETRIVSVNPGHENSLLYVVTPAIRDGKVGGVVLGAIDLAKRHFGSNQDGISSAVTL
                     IATRDGNFAYPPTPPALARDGKLGAWVAGGNGPLEARLWLNRDKWVVASAPLPGTDLV
                     YVLAAQDGVLFGKARQRMYVRTAVGLLLVIPPLFMLVLMLRRALRVFRQSEQETVERE
                     RLQVLADASNLIAHEVRNALNGLGVGLDLLLKGDRGAQQGREGRIVRQLRDEMQRLSE
                     FTGELMLFSKGIVPHPVRLDLADTVPKLTELSLDLASEAGCKIDVRVERSPVWTNADP
                     TLLRVVLGNLIGNALDVLAAHAPPSGGLVRILLEADDKQARLHVTDDGPGVPEAFRSH
                     LFEPFQTTKPSGTGLGLALSRRIARAHGGDLVLEASSSGASFLLTLPTETP"
     gene            complement(2551..>3459)
                     /locus_tag="VFQ35_11670"
     CDS             complement(2551..>3459)
                     /locus_tag="VFQ35_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9931343.1"
                     /translation="VQDYIETAMGGHVASELWDGERRFDVTVRLPRSTRADAESIRRI
                     MLPLDDGHLIPLAAVAQVAMGTGRAAITRENGRRYVGVRMNVRNRDLGSFVQEARDKV
                     NQSVTLPPGYEMKWGGEFENQQRAMERLKLVIPLSLLITFLLLFSAFQSVFDAVVILT
                     MVPFALIGGVIALALAHMPLSVSAAVGFIALLGQAVLNGVLVVAAIRERIAAGENLRD
                     AAMNGTRERLRAVLVTAFLASLGLLPAALSHAIGSETQRPIAVVVVGGTISAALLTLV
                     VLPVSYYVASVARQSLERSRLARAQA"
BASE COUNT          593 a         1126 c         1170 g          570 t
ORIGIN      
        1 tgactcgaag ctcgacctcg atttcacgtt gcccgccgac gcgacgaaag cgcgcgctgt
       61 ccaagaactt gaggagcttg gcctgagatg cttccggtag ctccgtgacc tcgtcgagga
      121 agagggtccc gccgttcgcc atctcgatga ggccgcggcg cgcggctttc gcatccgtga
      181 aggcgccgcg ctcgtgaccg aagagctcgc tctcgaccag ctgctccggg agggctgcac
      241 agttgacgtc cacgaaggga gcgccttcgt tgtcggcgta ggtgagccga tgcaaaagct
      301 ccgccgcgaa ctgcttaccg gcgcccgaag gcccaaggag cagcacgggc gtctgcgggc
      361 tgccgctcag ccgctcgagc tcggagtaca cttttcggag cgctgcgctc tgcggctcga
      421 tgccgattgg gcggcggtgc gcgacgttcg cgagagccag gcgctcgcgc agccctcgcg
      481 tctcgagcac cttgtggatc ttgatcagga ggtcggcgac gttgattggc ttcgtcaggt
      541 agtcgactgc gccggcttga atcgccgcga ccgcgctgtc gatgtcgccg taagccgtga
      601 cgacgatcac cgggatcacg gcgcctccgc tcgcgctgag ctcctgcagc aacgcgatcc
      661 cgtcgccgtc cggcaggcgg cggtcgagga tcatcacgtc gggcgcgccg ctgtcgagct
      721 cctcgcgcgc cttgcgcaga gtcgcggcgc tgcgtacctc gaatccttcg ccggcgaggc
      781 ccgcctcggt caaaatgcgg aagctgcggt cgtcgtccac aatcaatacg gtgggtttca
      841 tggggtctcc gtcggcagag tgagcaggaa cgaagcaccg gagctggacg cttcgaggac
      901 gagatcgccg ccgtgcgcgc gggcgatacg tcgagaaagc gcgaggccga gcccggtgcc
      961 gctcggctta gtcgtctgaa aaggttcgaa caggtggctc ctgaacgctt cgggcacgcc
     1021 ggggccgtcg tcggtgacgt gcaggcgggc ctgcttgtcg tcggcctcga gcaggattcg
     1081 aaccaagccg ccgctcgggg gagcgtgagc ggcgagtaca tcgagggcgt tgccaatcag
     1141 gttgcccagg acgacgcgga gcagcgtcgg atctgcgttg gtccacactg gcgaccgctc
     1201 gacgcgcacg tcgatcttgc agccggcctc gctcgccaaa tcgagcgaga gttcggtgag
     1261 cttgggcacg gtgtcggcca aatcgaggcg caccggatga ggtacgatgc ccttggaaaa
     1321 cagcatcagc tctccggtga actccgagag ccgctgcatc tcgtcacgca actggcgcac
     1381 gatgcggcct tcgcgtcctt gctgcgcgcc gcggtcgcct ttcaatagca ggtcgagtcc
     1441 caccccgagc ccgttcagcg cattgcgcac ctcgtgggcg atgaggttcg aagcatcggc
     1501 gagcacctgg aggcgttcgc gctcgaccgt ctcttgctcg ctttgtcgga acacgcgcag
     1561 cgcgcggcgc agcatcaaaa cgagcatgaa cagcggagga atgacgagca gcagcccgac
     1621 cgcggtgcgc acgtacatgc gctgtctggc tttgccgaag agcacgccgt cttgcgccgc
     1681 gagtacgtag acgagatccg tgccgggcag cggcgccgag gcgaccaccc atttgtcgcg
     1741 gttcagccag agccgcgcct cgaggggacc gttgccgcct gcaacccaag ccccgagctt
     1801 tccgtctcgc gccaaggccg gcggcgttgg tggatacgca aagttgccgt cgcgcgtcgc
     1861 gatcagagtg acggcgctcg aaatgccgtc ttggttgctg ccgaagtgcc gtttcgcgag
     1921 gtcgattgcg ccgagcacga cgccgccgac ctttccgtcg cggatggcgg gcgtcacgac
     1981 gtacaaaagc gagttttcgt ggcccgggtt tacggacacg atgcgcgtct ctcgggagcg
     2041 actgatgtcc ggaaaccagg gggccgattt gaacgacgtg ccctgcggca agaaggcgtg
     2101 aggctccgcc cacaccaccg cgccctcgag atcgaggatg gcgacgccga cgtcgaacag
     2161 cgtgctgcgc ttgtgcgcga tttcgaggag ctcgcgctcg ggggagctat cgttgtcctg
     2221 ggcttggatc tcggcgcgcg aagcgaggcg cccgagctcc gtggtcagga ggtcgaggta
     2281 gcggcggagc gcgtagctgc gatcgatcgc cgccatccgc atcgaattct cgagttgctc
     2341 ctcgtcgtgc gcctcttcgg tgacgagcac ggcgatgagg agcgacgcga ccaccactgc
     2401 cgtaccgacg taaacggcga gcgcccactg cgcgaggcgt gcgtgggcag cggccagcgt
     2461 gacggcggcg ttttgggagt tgttgtcgag tttggcttcg cgattcacgc gattcaccac
     2521 gagttacaaa gtatggaatc cgtacgacac tcaggcttgg gcacgggcga gccggctgcg
     2581 ctcgaggctc tgacgcgcaa cgctcgccac gtagtaagac acgggcaaga ccacgagggt
     2641 aagcagcgcg gccgaaatcg tgccgccaac caccacgacc gcgattggcc gctgcgtttc
     2701 gctcccgatc gcgtgtgaga gcgccgccgg caaaagcccg agagaagcca agaaagccgt
     2761 gaccagcacc gcccgcaagc gctcgcgagt gccgttcatc gcggcgtctc tcaggttttc
     2821 tccggccgcg atgcgctctc tgatggcggc caccacgagc acgccgttca gcacggcttg
     2881 cccgaggagc gcgatgaagc cgaccgccgc cgaaaccgag agcggcatgt gcgcgagcgc
     2941 gagcgcgatc acgccgccga tcaaggcgaa cggcaccatc gtcaagatca cgaccgcatc
     3001 gaacacggac tggaacgccg aaaacagcaa caagaacgtg atcaacagcg agagcggaat
     3061 cacgagcttc agccgctcca tggcgcgctg ctggttctcg aactcaccgc cccacttcat
     3121 ttcgtagccg gggggcaggg tcacgctctg gttcaccttg tcgcgagctt cttgcacgaa
     3181 cgagccgagg tcgcggttac gcacgttcat gcgcacaccg acgtagcgcc ggccgttttc
     3241 gcgggtgatc gcggcgcgcc cggtgcccat cgccacttgc gcgaccgcgg cgagcgggat
     3301 caggtgcccg tcgtcgagcg gcagcatgat gcgccgaatg ctctctgcgt cggcgcgcgt
     3361 cgagcgcggg agccggaccg tgacgtcgaa acgccgctca ccgtcccaga gctcgctcgc
     3421 gacgtggcca cccatcgcgg tctcgatgta gtcctgcac
//
LOCUS       DASTKS010000567         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_759069, whole genome shotgun sequence.
ACCESSION   DASTKS010000567 DASTKS010000000
VERSION     DASTKS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759069"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="VFQ35_11675"
     CDS             <1..1922
                     /locus_tag="VFQ35_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931344.1"
                     /translation="YRVTLRAGFPLLLGVVAAGSPQRALAAPPDAHFDVTDAASVLYN
                     FDNRDTRRGQVGTVANDHFGLLYNRLNLQVTSGKLTLMLRADSAWFYASRSPEEIADE
                     LASLESPSVAGTDYRLRKLTEAGIELSNRFINWTYPAKYAVTWGSPDFEVTLGDSYAQ
                     LGRGLVLSLRKLDELASDNTVRGARIAARFKTGDLRMKLTALGGSLNPLRLDEASGRY
                     LGVQSNVTPGFVALTEAGMPRAIGTAFTPLAGGCEQSGTCSFAPDRLLAAQVELAFPK
                     ATLSTQGSLVAREPALTQDTSRSASSILTVSQSLELPSFAQHGSAYFEGAFQRLDGAA
                     SAPDAGYALYGSVSWIEPRFSLVAEGKHYRRFFPLSANVSASRAREFALLAYSAPPTT
                     EEIWNDTQFGGLNTCVSGGRLKAEVHATRAYSVFTWLGHYRSFGESVSNDACDTRSEN
                     ENRIWDAAAGVNANLRTRPARYQIWMGTRFDDTTRTLSSPNGATEVFYRELYLRYSVS
                     EPLGGPFTLELQGFHRRRRETVGGPSDRWFEGQHSTAIDWGERLSAAIGVEYDSRPGV
                     PLGYVNAMLSYRPTSAWSFALFAGQRRGALRCVGGVCRVYPAFEGVRLDATFRYGAFS
                     AATMSRSRDSLEPVV"
     gene            complement(1859..2443)
                     /locus_tag="VFQ35_11680"
     CDS             complement(1859..2443)
                     /locus_tag="VFQ35_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9931345.1"
                     /translation="MSSSRKTPLTLWGRSGGRARASVVALGLALSTGACAPASGSATG
                     PAAAPNSARPPDFELNALDGSSVRLSEHLGKEVVLIDFWATYCEPCLRSMPALDALYT
                     KYKARGFVVFGIAIDGPDSLAQVRADVQRLGVHFPILLDQDTRVVALYNPKTSAPFSV
                     LIGKQGNVLRQKEGYTTGSSESLERDIVAALNAP"
     gene            complement(2443..3144)
                     /locus_tag="VFQ35_11685"
     CDS             complement(2443..3144)
                     /locus_tag="VFQ35_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9931346.1"
                     /translation="MMRPFTILLVSCAVFGCSSEPLSSSTPGDSAGGSTNAGGSSANG
                     GSATVPTLSRPDYPEGPYGHGVGATVPNLSFLGWRDPAAAQYDAEHLEVVNLSDFYNP
                     NGTEGKPRIIALNASAVWCTVCRAEYRQLQSSHTYANYRPKGVEMIGVLFEDNDYNPA
                     KPSDLNRWGTVFEVPFPLVLDPGFKTGQFFASDATPLNLLIDTSNMQILNVTMGYDAT
                     DPQAYWGAIDKWLSQ"
     gene            3244..4605
                     /locus_tag="VFQ35_11690"
     CDS             3244..4605
                     /locus_tag="VFQ35_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931347.1"
                     /translation="MRQSLNIGFAQGARRRFAALAALLSFASAAAAGCGSTKEKESPC
                     EDSSCLPGQRCTQGECRPTCASQDDCPKGQNCALYEFGDGSRGQYCVVLDYAKNGRTG
                     QLEACQADGECDTPRGFKCVDALCQRRTGQFEACEDDAQCDQPNGFSCVSGECRVPCA
                     SHFDCAPVGTCESTSQGMYCKSGTLSKRGQYYARCPYGNSDCDTTKGFTCIGSGVADL
                     DAFCTAACSSADDCPSGFRCGAVGATPCANTCGVTGQQVEGCIPASDIGPGKAYECGQ
                     PFGLIRRVCVRNAFCTTCRTDEDCFGISGQICAKDKSGQKICTFPCTPGIASCPWGAA
                     TECGVWDKERGVATCAHRFGACHGTGKGCEPCVDNIDCGKNGYCQRSSFTGERFCVDL
                     STSCNCGSDADANGSCEGHGCPESPGGLAMTCADTSLGQLCLGAQSGDSIGSVQTGCW
                     APP"
     gene            complement(4714..>5592)
                     /locus_tag="VFQ35_11695"
     CDS             complement(4714..>5592)
                     /locus_tag="VFQ35_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931348.1"
                     /translation="KIKANFKAGVSVTYVPGKCEVEAEAQLSCEASCKSDIMCTEPDI
                     SVRCEPGKLSVECSGECTGTLSCEGSASVAVKCEGACNGSCSGTCGGTCNGECDGTCS
                     AMDGEGKCAGTCSGTCKGSCSAKCTGTCSGSCTYDASASVKCDAKARCEGMCSVAGTA
                     PRCDGEITPPKCSGNASCQGSCEGKAKFNAKCTPPTVGITGFADAEFTATLTDNLPVV
                     FAVAEQAKVVADGAVTLANSTGQFLGQVGADLQCAAAHLTTVTSKLEASAKASVSVSV
                     SVMASASVSGAATTGG"
BASE COUNT          946 a         1876 c         1806 g          964 t
ORIGIN      
        1 gctatcgagt cacccttcgc gccgggtttc ccctcctcct gggtgtcgtg gccgcgggat
       61 cgccccagcg agccctcgct gcacctcccg acgcgcactt cgacgtcacc gacgcggcga
      121 gcgtcctcta caacttcgac aatcgagaca cgcggcgcgg ccaggtgggt acggttgcga
      181 acgatcactt cggcctgctc tacaatcgcc tgaacctgca ggtgacgagc gggaagctca
      241 ccctgatgct gcgcgccgac agcgcttggt tctacgcttc gcgctcgccc gaagagatcg
      301 ccgacgagct cgcctcgctc gagtcgccca gcgtcgctgg taccgactat cgcctgcgca
      361 agctcaccga agccggaatc gagctctcga accgattcat caactggact tatccagcca
      421 agtacgccgt gacctggggc tcgcccgact tcgaggtgac gctcggagac agctacgccc
      481 agctcgggcg cgggctcgtg ctcagcttgc gaaagctcga cgagctcgcc tccgacaaca
      541 ccgtgcgcgg agcgcggatc gcggcgcgtt tcaagaccgg tgacctgcgg atgaagctca
      601 ccgcgctcgg cggttcgctg aatccgctcc gcctcgacga ggcgagcggc cgctatctgg
      661 gcgtgcaaag caacgtgacc cctgggttcg tggccctcac ggaggcgggc atgccgcgcg
      721 cgatcggcac cgcgttcacg cctcttgccg gcggctgcga gcagagcggc acctgtagct
      781 tcgcgcccga tcgcttgctc gcggcacagg tagagctcgc ctttccgaag gcgacgttga
      841 gtacccaggg gagcctcgta gcgcgcgagc ccgccctcac ccaggacacc tcgcgcagcg
      901 caagctcgat tttgacggtg agccagtcgc tcgagctgcc gagcttcgct cagcacggct
      961 ccgcgtactt cgaaggcgct tttcaacgac tcgacggcgc cgcgagcgcc cctgacgcag
     1021 gctacgcgct ctacggcagc gtcagctgga ttgaaccgcg tttctcgctc gtggcagagg
     1081 gcaagcacta ccgccggttc ttccccctca gcgccaacgt gagcgcgtcg cgcgctcgtg
     1141 agttcgcgct gctcgcctac agcgcgccgc cgaccacgga ggaaatttgg aacgacaccc
     1201 agttcggcgg gttgaacacc tgcgtgagcg gcggacgcct gaaggcggaa gtccacgcga
     1261 cgcgcgccta ctccgtgttc acctggctcg gccactatcg gagctttgga gagtccgtga
     1321 gcaacgatgc ttgcgatacg cgctccgaga acgaaaatcg catttgggat gcggccgccg
     1381 gcgtgaacgc gaaccttcgc acccgccccg ctcgctatca gatttggatg gggacgcgct
     1441 tcgacgatac gacgcgcacc ctgagcagcc cgaacggcgc gaccgaggtc ttctatcgcg
     1501 agctctacct gcgctactcg gtgagtgaac cactcggagg accgttcacg ctcgagctgc
     1561 aaggcttcca tcgccgccgg cgcgaaaccg tgggcgggcc gagcgaccgt tggttcgaag
     1621 gacaacattc gaccgccatc gactggggcg agcgcctgag cgccgcgatt ggggtcgagt
     1681 acgacagtcg ccccggcgtg ccgctcggtt acgtgaacgc gatgctcagc tatcgcccga
     1741 cgagcgcctg gagctttgct ctcttcgccg gtcagcggcg tggcgcgcta cgctgtgtcg
     1801 gcggcgtctg ccgagtctac cccgctttcg aaggcgtgcg gctcgacgcc acgtttcgct
     1861 acggagcgtt cagcgcggcg acgatgtcgc gttcgaggga ctcactcgaa ccagtggtgt
     1921 agccctcttt ctgacggagg acgttgcctt gcttgccgat gaggacgctg aagggagcgc
     1981 tggtcttcgg gttgtagagg gcgacgaccc gcgtgtcttg gtcgagcaga atcgggaagt
     2041 gcacacccag gcgctgaacg tcggctctca cttgagcgag agaatcggga ccgtcgatgg
     2101 cgatgccgaa cacgacgaag ccgcgagctt tgtacttcgt gtagagcgcg tcgagcgccg
     2161 gcatcgagcg gagacacggc tcgcaatacg tcgcccaaaa gtcgatcagc acgacctcct
     2221 tgcccaagtg ctccgaaagg cgcacgctcg acccatcgag cgcgttgagc tcgaaatcgg
     2281 gaggccgcgc cgagttcgga gccgccgcgg ggcccgttgc cgagccagac gccggcgcac
     2341 acgcacccgt cgagagcgcg agcccgagcg ccacgaccga cgccctcgcg cgcccacccg
     2401 aacggcccca aagggtgagc ggcgtttttc gagagctgga cactactgcg acagccactt
     2461 gtcgatggcg ccccagtaag cctgggggtc ggtcgcgtcg tagcccatcg tgacgttcag
     2521 gatctgcatg ttgctcgtgt cgatgagcag gttcagaggc gtcgcatccg aagcgaaaaa
     2581 ttgacccgtc ttgaagcctg gatcgagcac cagagggaac gggacctcga acacggtgcc
     2641 ccagcggttg agatcgctcg gcttcgccgg gttgtagtcg ttgtcctcga agagcacgcc
     2701 gatcatttcg acgccttttg gacggtagtt tgcgtacgtg tgcgagctct gcagctgccg
     2761 gtactcggct cgacacacgg tgcaccatac cgccgacgcg ttcagggcga tgatgcgagg
     2821 cttcccttcc gtgccgttcg gattgtagaa atccgagaga ttgacgacct cgaggtgctc
     2881 ggcgtcgtat tgagcggcgg cggggtcgcg ccaaccgaga aagctcaggt ttggcaccgt
     2941 cgccccaacc ccatggccgt agggcccctc tggatagtcc gggcgcgaca gagtcgggac
     3001 tgtcgccgaa ccgccattcg ccgatgaacc gcccgcgttc gtgctgccac cagccgagtc
     3061 gccgggcgtg ctcgaactca atggctcact cgagcaaccg aacaccgcgc acgagaccag
     3121 caaaatagta aacgggcgca tcatggtgtg cgaggtagta tgcctcaatc ctccgcagcg
     3181 gggatgcact ttcctacccg gcacgccacg cgacctcgcc gtggccttat cgacggcatt
     3241 tcgatgagac agtctttgaa tatcggcttt gctcagggcg cccggcgacg cttcgccgca
     3301 cttgccgcgt tgttgtcgtt cgccagcgcc gccgccgccg ggtgcggctc taccaaggag
     3361 aaggagtccc cgtgcgagga ctcgagctgc ctgccgggac agcgttgcac gcagggcgaa
     3421 tgccgcccga cttgtgccag ccaagacgac tgtcccaagg gacaaaactg cgcgctctac
     3481 gagttcggtg acggctcgcg tggacagtat tgcgtggtgc tcgactacgc gaagaacggg
     3541 cgcaccggac aactcgaggc atgccaagcg gatggcgagt gcgacacgcc gcgtggcttc
     3601 aagtgcgtcg acgcgctttg ccagcgccgc acgggacagt tcgaagcgtg tgaagacgac
     3661 gcgcaatgtg accagccgaa cgggttctcg tgcgtgagcg gcgagtgccg cgtcccctgc
     3721 gcgtcacact tcgattgcgc acccgtcggt acgtgcgaga gcacctccca ggggatgtat
     3781 tgcaagtccg gaacgctttc caaacgaggc cagtattacg cccgctgtcc ctacggtaac
     3841 tccgattgcg acacgacgaa gggtttcact tgcatcggct ccggcgtcgc ggatctggac
     3901 gccttctgca cggccgcgtg cagtagtgcc gacgattgcc cgtcgggctt tcgctgcggc
     3961 gcggtgggcg cgacaccgtg cgccaacacc tgcggcgtga cggggcaaca ggtcgaaggt
     4021 tgcatccccg cgagcgacat cggccccggg aaagcctacg agtgcggtca acccttcggg
     4081 ctcatccgcc gcgtttgcgt gcgaaatgct ttctgcacga cgtgtcgaac cgacgaagat
     4141 tgcttcggca tctccggaca gatctgcgcc aaagacaaga gcggacagaa gatctgcacc
     4201 ttcccctgca cgccggggat cgcgtcctgc ccctggggcg ccgcgaccga gtgcggagtc
     4261 tgggacaagg agcgcggcgt cgcgacgtgc gcccaccggt ttggcgcctg tcacggcacc
     4321 ggcaaaggct gtgagccgtg cgtcgacaac atcgactgcg gcaagaacgg gtattgccag
     4381 cgctcgagct tcacgggcga gcgcttctgc gtcgacctct ctacgtcgtg caattgcggc
     4441 agcgacgccg acgcgaacgg ctcctgtgaa ggccacggct gccccgagtc ccccggtggt
     4501 ttggcaatga cgtgcgccga cacgagcctc ggtcaactct gtttgggcgc gcagagcggc
     4561 gacagcatcg gcagcgtcca gaccggctgc tgggcgccgc cctgagcgac cgcgcggagc
     4621 gcgtctgcga gcgcagccga ctcacaaaat gcgaccggcg ccgtcccgac tcgggaggcg
     4681 ccggtcttct tcgatgcgac taggggcggg tgctcagccg ccagtcgtgg ccgcgccgct
     4741 gacgctggcg ctcgccatca cggagacgct gaccgagacg gacgccttcg cgctcgcctc
     4801 gagcttcgac gtgacggtgg tgaggtgtgc ggcggcgcac tgcaggtccg cgcccacctg
     4861 acccaggaac tgacccgtcg aattcgcgag cgtgaccgcg ccgtcggcaa ccaccttcgc
     4921 ctgctcggcg acggcaaaca ccaccggcag gttgtcggtg agcgtagccg tgaactcggc
     4981 gtcggcaaaa cccgtgatgc caaccgtggg cggagtgcac ttcgcgttga acttcgcctt
     5041 gccttcgcag ctaccctggc agctcgcgtt gcccgaacac ttcggcgggg tgatctcgcc
     5101 gtcgcagcgc ggagcggtgc cggcgacgct gcacatgcct tcgcaacgag ccttggcgtc
     5161 acacttgacg ctggcgctcg cgtcgtaggt gcagctgccc gagcacgtcc cggtgcactt
     5221 ggccgagcac gagcccttgc aggtcccgct gcaagttccg gcgcacttac cctcgccgtc
     5281 catcgccgag caggtgccgt cgcactcgcc gttgcaggtt ccaccgcaag taccgctgca
     5341 gctgccgttg caggcgcctt cgcatttgac cgcgacgctc gcgctgcctt cacagctgag
     5401 cgttccggtg cattctccgg agcactcgac cgagagcttg ccgggctcgc aacgcacgct
     5461 gatgtcgggc tcggtgcaca tgatgtccga cttgcagctc gcttcgcacg agagctgtgc
     5521 ttcggcttcc acttcgcact tgccgggcac gtacgtgaca ctcacgccag ccttgaagtt
     5581 ggctttgatc tt
//
LOCUS       DASTKS010000568         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_91013,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000568 DASTKS010000000
VERSION     DASTKS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91013"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VFQ35_11700"
     CDS             <1..1131
                     /locus_tag="VFQ35_11700"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HET9931349.1"
                     /translation="HAYGSVLERGRYPRGAVYLELPFELVDVNVHPQKAEVRFADPRA
                     VGDALYGILSRALAGAFSLPPRRPAARPPTPNPAATAARLPGAVAPLAEPSTRAADWM
                     EALSAAASTASALEHPSAASPGSAANAPSSAASADAHSFSSAGAKSGPSPSESAVSRA
                     AEPPPAVLAFRDSSSARIRPSENVTWASLRFVAQLRQTYLLCEGTDGLYLLDQHAAAE
                     RVTFTRLRRDYQARAVPSQSLLFPAMVEVTASEAELIEARAKEFAEVGMDVRVRGPES
                     VSVHTVPKLLARSSPERMLRDLLTEVTRKGERAFSDAVDLALATMACHGSVRAGDTLA
                     ESEVSALLVALDDADFAGHCPHGRPVVTFIAWAELERKVGRR"
     gene            1157..2053
                     /gene="miaA"
                     /locus_tag="VFQ35_11705"
     CDS             1157..2053
                     /gene="miaA"
                     /locus_tag="VFQ35_11705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9931350.1"
                     /translation="MPTSPEKPPLLVVVGPTASGKTELATRLCESQGGEIVTADSVQV
                     YRGFDIGSGKPSRAERERVRHHLIDVVEPNEALDAARWAELAEASIADIRARGKVPIV
                     CGGTFLWVRALIYGLAAAPPASAEIRARHRELAERDGRAALHAELARVDAEAAARLAP
                     NDFVRVSRALEVFELTQVPISRWHAEHGFREKRHEARLIGVRREKSELDALIEKRVTN
                     MLDAGWVDEVAELIRAGHGESRAMGAVGYKQIKEALALGHIDREALAQGIVRVTRVFA
                     RRQRTWLRDEPVEYVAPAAALS"
     gene            complement(2083..2670)
                     /locus_tag="VFQ35_11710"
     CDS             complement(2083..2670)
                     /locus_tag="VFQ35_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931351.1"
                     /translation="MSLEGGSDDPVSLTNYLLAAIATLPTFHEDVGAQYVARKHAQAQ
                     VIAQAISDVAESTEGWSGTKRELATLLLTVAWHETHFSLRIHEGNCKPFECDHGRARS
                     LWQLHVHPSLPRETWLGLAGVDVDATRRAAREAAKALARSRNMCASRARGVELVAQTL
                     SAYAGRGCARLLPDIDARVRTYRRLILIQPKGTSA"
     gene            3165..>3359
                     /locus_tag="VFQ35_11715"
     CDS             3165..>3359
                     /locus_tag="VFQ35_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931352.1"
                     /translation="MSSRRFRAPCHHHRDRSANAGSPPPRLQRETPRLRHRACDAKRR
                     ICATAPQRETPRHNCGDRNAK"
BASE COUNT          569 a         1174 c         1070 g          546 t
ORIGIN      
        1 cacgcttacg gcagcgtgct cgagcgcggg cgctatccgc gcggcgccgt ctacctcgag
       61 ctcccgttcg agctcgtcga cgtcaacgtg cacccgcaaa aggcagaggt ccgtttcgca
      121 gatccgcgcg cggtcggcga cgccctctac ggcatcctgt ctcgcgcgct cgcgggcgca
      181 ttctcgctgc cgccgcggcg acccgcagcg cgcccgccga ctccaaatcc cgcggccacg
      241 gccgcgcgcc ttcccggtgc ggtagcgccg ctcgccgaac ccagcacgcg cgcagccgat
      301 tggatggagg cgctttctgc cgctgcaagc acggcttcgg ctctcgaaca cccgtctgcg
      361 gcaagcccag gctcggcggc gaacgctccg tcctccgcgg ccagcgccga cgctcactcc
      421 ttctcgagcg ccggcgcgaa gtctggcccg tcgccgagcg aatcggcggt gtctcgcgcg
      481 gccgagccgc cgcctgccgt gctcgcattt cgcgactcct cgagcgcgcg cattcggccg
      541 agcgagaacg tcacgtgggc gagcctgcgc ttcgtggcgc aacttcgaca aacgtatctc
      601 ctctgcgaag gcacggacgg cttgtacttg ctcgatcagc acgcggccgc ggagcgcgtc
      661 actttcacgc gtctacgccg cgactaccaa gcgcgcgcgg tgccgtcgca gtcgttgctc
      721 ttccccgcga tggtcgaggt caccgccagc gaggccgagc tcatcgaagc gcgggccaaa
      781 gagttcgccg aagtcggcat ggacgtccgt gttcgcggtc ccgagtcggt gagcgtgcac
      841 accgtgccga agctcctcgc gcgctcgagc ccagagcgca tgctgcgtga cctgctcacc
      901 gaagtgaccc gcaagggcga acgcgccttc tcggatgcgg tcgacttggc gctcgccacg
      961 atggcgtgtc acggcagcgt gcgcgccgga gacacgctcg cggaaagcga agtcagcgcg
     1021 ctgctcgtcg cgctcgacga cgccgacttc gccgggcact gcccgcacgg gcgccccgtc
     1081 gtcaccttca tcgcttgggc agagctcgag cgcaaagtcg gtcggcgata gcggctcgtc
     1141 gtcgtcgctt ccatccatgc ccacgagccc cgaaaagccg cctctcttgg tcgtcgttgg
     1201 gccaaccgcc tccggcaaga ccgagctcgc gacgcgcctc tgcgagagcc agggcggaga
     1261 gatcgtcacc gccgacagcg tgcaggttta tcgcggcttc gacatcggga gcggcaagcc
     1321 gagtcgcgct gaacgtgagc gtgtccggca tcacctcatc gacgtggtcg agccgaacga
     1381 agcgctcgac gctgcgcgct gggccgaact cgccgaggcg agcatcgcgg acattcgggc
     1441 ccgcggtaag gtcccgatag tctgtggtgg cacctttctg tgggtgcgcg cgttgattta
     1501 cggcctcgcc gcagcgccgc cggcgtcggc cgaaattcgt gctcgacacc gcgagctagc
     1561 cgaacgcgac ggacgcgctg cgctccacgc cgagctcgcc cgtgtcgatg cggaggcggc
     1621 cgcgcgcctc gcccccaacg acttcgtgcg cgtcagccgc gcgctcgagg tcttcgagct
     1681 cacccaagtg ccgatcagcc gatggcacgc cgagcacggc ttccgggaga agcgccacga
     1741 agcgcgtctg atcggagtgc gccgcgaaaa atcggagctc gatgcgctca tcgagaagcg
     1801 ggtgaccaac atgctcgatg cgggctgggt cgacgaggtc gcggagttga tccgagcggg
     1861 ccacggagag agccgcgcga tgggcgccgt cggctacaag cagatcaaag aagcgctcgc
     1921 gctcggccac atcgatcgcg aagcgctcgc ccaggggatc gtccgcgtga cgcgcgtgtt
     1981 tgcgcggcgt cagcgcacgt ggctccgcga cgagcccgtc gagtacgttg ccccggctgc
     2041 cgcgctctcg taacggccag ggcgccgcgg agcgcaacgc ctctacgcgg acgtcccctt
     2101 cggctgaatc aatatcaggc ggcggtaggt gcggacacgc gcgtcgatgt ccggcaaaag
     2161 ccgggcgcag ccgcggcccg cataggcaga caacgtttgc gccacgagct cgacgccgcg
     2221 agctcgactc gcgcacatgt tccggctgcg agcgagcgcc ttggccgctt cgcgcgccgc
     2281 gcgccgcgtg gcgtccacgt cgaccccagc gaggccgagc cacgtttcgc gcggcagtga
     2341 ggggtgcacg tgcagttgcc aaaggctgcg tgcgcggccg tggtcacact cgaaaggttt
     2401 acaattccct tcgtgaattc tcagtgagaa gtgcgtctcg tgccatgcca ccgtaagcag
     2461 caaagtggcc aattcacgct tcgttccact ccagccctcg gtcgactcgg cgacgtctga
     2521 aatcgcctga gcaatcactt gagcttgagc gtgttttctc gccacgtatt gtgcaccgac
     2581 gtcctcgtgg aacgtcggca acgtggctat ggctgccaag agatagttgg taagactcac
     2641 gggatcatcg ctcccgccct cgagcgacat gcgagttgat tcgcaatcgc gtgatccctt
     2701 ttagcactgt atttcgaaaa tcaacgcgtg atcgtcgtag tcaatgcagt ggcatttcac
     2761 cccgtttcac actccgcgcg aactattcga attacaacga catacgcgcg ggccggccag
     2821 ggggtcgagt tcggcgattt tcgcgtattc catatacgca acattcgtaa atcaagcgtg
     2881 ctgaaacaac ggggcaaatc tgcgttcgaa cctccacgtc gcacgataat actgaggggt
     2941 tattttcgca cacgcagtgc gaccgctgcg tttcgcgcac agcgtgagca actcgaaact
     3001 cgcgagctca ctgcggctcg catcgacgcc gccagtacga gcgcgcgtga agccggcgat
     3061 ggagcttcgg cggctcgccg cgcgacgagc tcgatcgaga atcgagatcg cgaattaccg
     3121 acacgcgctc ctcgtgcttc gtgctgcaaa gctcctcagt gaccatgagc tcacgacgtt
     3181 tccgcgcgcc gtgtcatcac caccgcgacc gcagcgcgaa cgcggggtca ccaccaccgc
     3241 gcctgcaacg cgaaacgccg cgtctgcgcc accgcgcctg cgacgcgaaa cgccgcatct
     3301 gcgccaccgc gccgcaacgc gaaacgccgc gtcacaactg cggtgaccgc aacgcaaag
//
LOCUS       DASTKS010000569         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1876430, whole genome shotgun sequence.
ACCESSION   DASTKS010000569 DASTKS010000000
VERSION     DASTKS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876430"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFQ35_11720"
     CDS             complement(<1..640)
                     /locus_tag="VFQ35_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9931353.1"
                     /translation="MLPFAQTGSVAPGVAWLRVSKTTELFRGMKAEHGITLTCAGRSL
                     SRVDGKERLHTPGALGLTQPGQVHCNVRRDTPGSFQLVSFDARRIEEARAALEQRGPG
                     LLESDSVDPSDQRARPLRRLHQVVLSGSADRFALDVAITEALTAFTSFLVGTRASHAG
                     LRPSVRRARDFLLHHIAEEVTLDAVAEHARTDKFHLCRAFSRELGLPPYAFLT"
     gene            742..1470
                     /locus_tag="VFQ35_11725"
     CDS             742..1470
                     /locus_tag="VFQ35_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931354.1"
                     /translation="MKKKKTALVTGANKGIGHETARRLAELDLTVLMGARDQGRGEAA
                     VDGLRNNSRDVQLLILDVADEQSIARAAASVAERFGRLDILVNNAGISLGAGLPSNQG
                     LEAMRSIFATNVFGPIAVAQAFLPLLERAPAARIVNVSSSLGSLGLAADPESPSAQLG
                     RLFGYAASKTALNAFTLWLANELRSKHVKVNAACPGYVATDLNRHKGPRTVEQGAEII
                     VRLATLPDDGPTGGFFDDAGSVPW"
     gene            1665..1865
                     /locus_tag="VFQ35_11730"
     CDS             1665..1865
                     /locus_tag="VFQ35_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931355.1"
                     /translation="MEPQHDTDYLGDGQGCIDPGSLKRAAADGCQTRGLVLTEIFPVT
                     TESCPNGAVEAKYQCCPASGLL"
BASE COUNT          366 a          640 c          702 g          335 t
ORIGIN      
        1 gagtcaaaaa cgcgtaaggc ggaaggccga gctctcgcga gaaggcgcgg cagaggtgaa
       61 acttgtctgt gcgcgcgtgc tcggcaactg cgtcgagcgt gacctcttcg gcgatatgat
      121 ggagcagaaa gtcgcgtgcg cgtcgcacgc tgggtcgaag cccggcgtgc gaggctcgcg
      181 tgcccacgag gaacgacgtg aacgcggtga gggcttccgt gatggccacg tccagcgcga
      241 agcgatccgc tgacccggag agcacgacct ggtgcaagcg gcggagcgga cgcgctcgct
      301 ggtcgctggg atcgaccgag tcgctctcga gcaggcccgg acccctctgc tccaaagcag
      361 cgcgcgcctc ttcgatgcgt cgcgcatcga aggaaacgag ctggaacgac cccggtgtgt
      421 cgcggcgcac gttgcagtgg acttgaccag gctgcgtgag gccgagcgct ccgggggtgt
      481 gcagacgctc tttgccgtcg acacgcgaga gcgagcgacc ggcgcaggtg agggtgatgc
      541 cgtgttcagc cttcattccg cgaaagagct cggtcgtctt gctcacgcgg agccaagcta
      601 cgccaggagc gacggatccc gtctgggcga acggcagcac gtcctcaatc tagcgcttcg
      661 gtgggtcctg cgcttgggcg ttcttggcac aagtcaagag cgcccaagat cgcgagcatg
      721 cggttcatga atgatcggac catgaagaaa aagaagacgg cgctcgtcac gggcgccaac
      781 aaaggaatcg gtcatgaaac cgcgcgccgc ctcgctgagc tcgatctgac ggtgctcatg
      841 ggagcgcgcg atcaagggcg aggcgaagcg gcagtcgacg gtctgcgcaa caattcgcga
      901 gacgtacagc tcctgattct cgacgtggcg gacgagcaga gcatcgcccg cgccgccgcc
      961 tccgtcgcgg agcgctttgg gaggctcgac atcctcgtga acaacgccgg catctcactc
     1021 ggcgcaggtc taccttcgaa ccaggggctc gaggcgatgc gcagcatctt tgcgaccaac
     1081 gtgtttgggc cgatcgccgt agcgcaggcg ttcctaccgc tgctcgaaag ggctccggca
     1141 gcgcgcatcg tcaacgtctc gagctcattg ggatcgctcg ggctcgcggc cgaccccgag
     1201 agcccgagcg cgcagttggg ccgactgttc ggttacgcgg cctccaaaac agcgctcaat
     1261 gctttcacgt tatggctcgc gaacgagctg cgcagcaagc acgtcaaggt aaacgcggct
     1321 tgtccgggat acgtggcgac ggacctcaac cgccacaagg ggccgcgaac ggtcgagcag
     1381 ggcgccgaga tcatcgtccg gctcgcgacc ctcccggacg acggtccgac gggcggcttc
     1441 ttcgacgacg ccggaagcgt cccgtggtga cgcagccttt gctctttcct agccatccgc
     1501 tgcggagaag tgggccgcgt tgacgtctcg tgctctgtag ctttcgcaag gctaattggc
     1561 aggggcgtgc gccgtcgcgt cgtatcgaca tcttacctcg cggtgctttg cgcaagcctc
     1621 cgcgtagctg cgcgactaga tgaaggcgta agagtcgtcg cggcatggag ccgcagcacg
     1681 ataccgacta tctcggcgac gggcagggtt gcatcgaccc ggggagcctg aagcgtgcgg
     1741 cggccgatgg ctgccagaca cgcgggctcg tcctgacaga aatcttcccc gtcacgaccg
     1801 agagctgccc gaacggcgcg gtggaggcga agtatcagtg ctgtcccgcc tccggcctac
     1861 tttgactcgg cgccggtcgt aacgtgagct ggaaggtggc gtcgccctcg gcgctggttg
     1921 ccggccgaga ggcgactgca cgcgaccgcc gagtgcacga agaacgcctc gggattggcg
     1981 accgccaagg cgcgccgcgc gtcgtgagcg cttgatagcg tatactcgtc gcggcctcat
     2041 gcg
//
LOCUS       DASTKS010000570        11244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_91137,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000570 DASTKS010000000
VERSION     DASTKS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91137"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VFQ35_11735"
     CDS             <1..1704
                     /locus_tag="VFQ35_11735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET9931356.1"
                     /translation="QLINAIRRDVPRPTVHARNLFHTSLAMWDAWAAYDSVADGYVHA
                     ERETADDVEAARAEAISYAAYRVLSHRYGNAVGGPVSQACFDAFMDKLGFDASDTHTE
                     GHEPRALGNRIGEAIIAAFANDGANETANYASPEPVPSGVPNLVVDVPGTKTTDPIRW
                     QRLVIAQAVTQNGIPQGAGAQDYVGPHWGQVTPFGLVRPAPDSAYLDIGAPALSVDDQ
                     LIDETVESIRKSAELDIEDDAFIDISAGAYGNNPLASNAGTGHPENPVTHQKYAPERV
                     KRGDFSRVLAEFWADGPRSETPPGHWNVLANGVSDNPLAAHKLFGAGAALDPLAWDVH
                     VYFALNGAVHDAAIAAWELKRKYQTARPITLVRYFGALGQRTDPTLPSYDQRGLPLVD
                     GLIELVTDESSRPGERHAHLRRYIGELAVRSWRGEPGDRAHEHGGIGWVRAVEWMPYQ
                     RRTFVTPAFPGYVSGHSTFSRAAATVLATITGDDFFPGGLATFTAKPGYLVFEQGPSA
                     QLELQWGTYYDAADQAGQSRIVGGIHTQRDDFDGRRIGALVGARAIERARPYFDGTAS
                     P"
     gene            1865..4459
                     /locus_tag="VFQ35_11740"
     CDS             1865..4459
                     /locus_tag="VFQ35_11740"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9931357.1"
                     /translation="MSNSSYVLVLHAPQQPPAYREFSQPRIVIGREAGDLVLPDPQCS
                     STHAEVLFDGANVRFRDLNSTNGTWFNGQRIGEMSWSAGASLQIGHHYLTLHEVRAAV
                     NARGRTAIGNAAQYGLGAPPAAAPGPNPHAPQPGAYGQQPNPYGQQPNAYGQQPNPYG
                     QQQNAPGQQPAYGQQPNPYGQQPSAPGQQPNAYGQQAYAAAAAPAAKKKKSNAIWIVL
                     PLVLVGTCGAGAAGLYWIGSNATEAERGGEAATLLDAPREATVKFVWFKGPPGPNASG
                     GTSPARVRVAPNRSGVVSVGVAEEFAGGGGNQWRTATWLAAFNATRTLNASLSDYEFN
                     VHVGGHTDGPSAGMLTTTTMLSLLRNKPMREDSTMTGTINPDGTAGPVGGIVQKMGGA
                     KESGLKRFGFPIGTRNHTDLKTQAQVDLVNVGQQLGLETREISDVYEAYEFMTGDKLP
                     RVEGVAENELEPSDQTQALLRAKVSGWKVRVEREIGALKEEVGRSGKAVQFAAPLLAQ
                     AERAYENAKRYESNSFLVSALDGYAETAILVASATRFTKIVPLMFQRNVPALLEVLES
                     AKTVNGDVDAFAQELEVRAQSKTRGGQVSTTFAFGHYVTARSATMIGDDFRDSAVRLI
                     QGIANGNVAADSNALVAVLERTTMPILYYDIARVYLEYAKDVQGLIADEGDRKPLTPQ
                     SVDRLVAGYASASGAVLAYFDALIVEEIANENSMTADQAKFAMAQREPEYYLTLKSNM
                     LSEYTGKTGASDGVKLMRLAAAGTAYLGGANLVNKWYSLGMNFDKSGNLVLENRRALS
                     AELDLARRNAREAAARAKAAGGFVPTAARLAFQVGSARREGNDEDKLAALSAYWQSSF
                     WSDLVAAQ"
     gene            complement(4479..6251)
                     /gene="argS"
                     /locus_tag="VFQ35_11745"
     CDS             complement(4479..6251)
                     /gene="argS"
                     /locus_tag="VFQ35_11745"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9931358.1"
                     /translation="MNHPVARLSELVLAAVVKCFGAEHASVDPAVHRSNFADYQADVA
                     MKLARALKKPPIEVARTVAAAIEAPELCESVQASAPGFVNFTLRREVLEAAVSELLTD
                     ERAGVPRSGTSETVVVDYPSPNAAKEMHVGHLRSSVIGDCLTRVLGFLGQRVIRQNHI
                     GDWGTPFGMLIEHLIDLGSEEARSQLEVGDLNAFYKAARQKFDADEAFAERARLRVVA
                     LQAGEAETLRLWQHLIDSSLKYFRTIYDKLGLTLEDSDVRGESFYNPFLAEVANELER
                     IGVAQVDQGALCVFSPEFKGREGEPLPLIVRKQDGGFGYAATDLAGVRFRTRELGATR
                     ILYVVGSPQQQHFAMVFSAARRAGYLPENVRAEHVAFGSVLGSDKKLLRTRSGDSPRL
                     MELLNEAVERVDAVIRDKNPELPAAERASVSELVGIGALKYADLSSERIKDYIFDWDR
                     MLAFEGNTGPYLQYAHARLCSIFRKAESEHAVAFAGALLRGEAAPGVSARVEDKRERE
                     LVLALLDFAGAVDAVATSLQPHRLCTYLYQLATAYTAFYEGCPVLKAPTSEQRDARLL
                     LCAATRQTLKLGLGLLGMGAPERM"
     gene            6498..7046
                     /locus_tag="VFQ35_11750"
     CDS             6498..7046
                     /locus_tag="VFQ35_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931359.1"
                     /translation="MQSSDVNGPSDAFFRVCSTCKKPLPFGERYYRCSVSTCNRPRTA
                     LYFCSVACWDAHVPEARHRDAWAEEVRAPTRAEWQAQQAEAEAERQRSEAPMTEKSPT
                     ERRIVAPKATGEAEREILVVVSKLKAYIKARSGFNTSDAVSEVLSDHLRRLCDLAIDN
                     ANVDNRKTVLDRDFKPLFKVGP"
     gene            complement(7063..7887)
                     /locus_tag="VFQ35_11755"
     CDS             complement(7063..7887)
                     /locus_tag="VFQ35_11755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9931360.1"
                     /translation="MSQKGGPLAAPEPWDLVSKGYAEEASYLMRAFSVRAIELMEPGV
                     SARVIDVAAGPGTLTLELAQRVASIDAIDFAPAMIEELQAALVTRNITNVTARVADGQ
                     SLPFGDERFDFGFSMFGLMFFPDRPRGYSELLRVLKPGGRAVVSSWAPLMDSSLMRGM
                     FGAIHEVDPNVPPPQRDPLGLENPDVLAAELTAAGFRDVAVEAVTRFVPPITSAEEVW
                     HRMSRSSAPLVLLRNRLGESEWQRGTERARAFLERYLRENPGELSSTALLGYGTKP"
     gene            complement(7972..8295)
                     /locus_tag="VFQ35_11760"
     CDS             complement(7972..8295)
                     /locus_tag="VFQ35_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931361.1"
                     /translation="MRLWLVLTAAILFYAWLDLRLNPRLLMETHFSMRPDLDQLPDLM
                     HWGGRLLNHRALSLLVGNALVLGGVGALFGRGIVRFVQRVREREASVSIVPSPPPTSV
                     PPAPR"
     gene            complement(8443..9690)
                     /locus_tag="VFQ35_11765"
     CDS             complement(8443..9690)
                     /locus_tag="VFQ35_11765"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HET9931362.1"
                     /translation="MLERLTSALGALVMLGLAFALCPRERRRSVNGRSVGGGMLLLLA
                     IGGLVLKTPLTRLFSFANRAVELLLGFTQDGARFVFGALVADPKTFGYIFAFQVLPTI
                     LFFSALMSLLYHVRVMPWVIQRCGRLLARTLNVSGAESFSTVADVFVGQTEAPLVIRP
                     YLRNMTASEINACMVAGYATTAGGVLAAYVAMLGRDIPGIAGHLIACSVMSAPASLVI
                     AKLMLPETETPETSKDAKVALPEAHANALDAVAAGAVDGLKLAANVAAMLIVFLAFTA
                     LADALLHGIGRAVGIELGLERILSVAFAPLAWLLGVPAGDVTKIAGLLGQKTLFNEFI
                     AYSHLAEMLHQDPAWISDRGRVVASYALCGFANFGSVGIQIGGYSALAPERRGDISRL
                     ALRAMFGGLLTTCLVACVAGMFL"
     gene            complement(9697..11037)
                     /gene="argG"
                     /locus_tag="VFQ35_11770"
     CDS             complement(9697..11037)
                     /gene="argG"
                     /locus_tag="VFQ35_11770"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET9931363.1"
                     /translation="MSRIYRSLPPAGTRLGIAFSGGLDTRCAVAWLTRNGMKVYSYTA
                     DLAQPDETDCSVIPKIALEHGAVEARLIDCREALVHEGIVAIQCGAFHLSSGGKKYFN
                     TTPLGRAVTTTAIIRAMRADDVHVFGDGSTHKGNDIQRFYRYGILTNPELKIYKPWLD
                     QKFVDAFGGRKEMSEYLNSIGLPHTMSVEKAYSTDSNVLGATHEAKDLERLDTGMRIV
                     EPIMGVAHYRPDVAIKTEEVTVEFNDGLPVSINGRRYDSLFQLFSEANRIGGRHGLGM
                     SDQIENRVIDAKSRGIYEAPGMALLHIVWERLLSAVHNENTLDLYFTLGRRLGRVLYE
                     GKWFDPEAMMLKDALTRWLAPSVSGSVTLELRRGDDYSILDTKAVYMAYGPEKLSMER
                     VEDPAFTPADRIGALEMQNLSVLDNRAFLVHHMDSIARLGGGDNPLPELLGKKS"
     gene            complement(11034..>11244)
                     /locus_tag="VFQ35_11775"
     CDS             complement(11034..>11244)
                     /locus_tag="VFQ35_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931364.1"
                     /translation="ERARAVSNRAGMPFPGRGTKFECKLGNGAREGPSPSVGGGEQAA
                     NGSTFLRVHVGVAQRPWDRATARPS"
BASE COUNT         2082 a         3855 c         3546 g         1761 t
ORIGIN      
        1 cagctcatca acgcgatccg ccgcgacgtc cctcgaccga cggtgcatgc gcgcaatctg
       61 tttcacacgt cgcttgcgat gtgggacgcc tgggctgcat acgacagtgt cgccgacggc
      121 tatgttcacg ccgagcgcga aaccgccgat gacgtagagg ccgcgcgcgc tgaggcgatc
      181 tcctacgccg catatcgcgt gctcagccat cgttacggca acgccgtcgg gggccccgtt
      241 tcacaggcct gcttcgacgc gttcatggat aagctcggct tcgacgcgag cgacacgcac
      301 acggagggcc acgagccgcg cgcgctcggg aaccgcatcg gtgaggccat catcgccgcc
      361 ttcgcgaacg acggcgcgaa cgaaaccgca aactacgcga gccccgagcc ggtgccgagc
      421 ggcgttccga acctcgtcgt cgacgtgccc ggcacgaaga ccacggatcc aatccgttgg
      481 caacgcctcg tcatcgctca ggccgtcact caaaacggca tcccgcaagg cgccggtgcg
      541 caggattacg tgggccccca ctggggccag gtgactccct tcgggctggt tcgaccagca
      601 ccagacagcg cctatctcga catcggagcg cccgcactca gcgtcgacga tcagctcatc
      661 gacgagaccg tcgagtcgat tcgcaagagc gccgagctcg acatcgagga cgacgcattc
      721 atcgacatct cggcgggcgc ctacggcaac aatccgctcg ccagcaacgc gggcaccggt
      781 cacccggaga acccggtgac gcaccagaag tacgcgccgg agcgcgtcaa acgcggtgac
      841 ttctcgcgcg tgctcgccga gttctgggcc gatggtcccc gctccgaaac gccgcctggc
      901 cactggaacg tcctcgcgaa cggcgtctcg gacaatccgc tcgcggccca caagctcttc
      961 ggcgccggcg ccgcgctcga cccgctcgct tgggacgtcc acgtctactt cgcgttgaac
     1021 ggcgccgtgc acgacgccgc cattgccgcg tgggagctca agcgaaagta tcaaaccgca
     1081 cgacccatca cgctcgtgcg ctacttcggc gcgctgggtc agcgcaccga cccgacgctt
     1141 ccctcttatg accaacgcgg tttaccgctc gtcgacggct tgatcgagct cgtcacggac
     1201 gagagctcgc gcccaggcga gcggcacgca catctacgcc gctacatcgg cgagctcgcg
     1261 gtgcgctcgt ggcgcggtga gccaggcgat cgggcccacg agcacggcgg catcggctgg
     1321 gtgcgcgcgg tcgaatggat gccgtatcag cggcgaacgt tcgtcacgcc cgcattccct
     1381 ggctacgtct cggggcacag cacgttcagt cgcgccgccg cgacggtgct cgcgaccatc
     1441 accggcgacg actttttccc cggcgggctc gccaccttca cggccaagcc cggctatctg
     1501 gtcttcgagc aaggcccgtc ggcgcaactc gagctccaat ggggcaccta ttacgacgct
     1561 gccgatcaag ctggccagtc gcgcatcgtc ggcggcattc acacgcagcg ggacgacttc
     1621 gacggccgcc gcatcggagc gctcgtcggc gcccgcgcca tcgaacgtgc gcgaccgtac
     1681 ttcgacggta cggcgagtcc gtaacgcgga gattcgcggc gaggtcgaat gtttccctac
     1741 acgggagctc acgctccgtg agcgaaacgc agcgatgaaa tcgcacgaaa gtgcgtgacg
     1801 gcgacgacgg ccggcaaacc cggtacaatc atcggtctct cttcatcggg accgtcaaag
     1861 tcgcatgtcc aacagctcgt atgtgctcgt cctccacgcg ccgcagcagc cgccagccta
     1921 tcgagagttt tctcaaccgc gaatcgtcat cggcagagaa gcgggtgacc tcgtgcttcc
     1981 ggatccgcag tgttcatcca cccacgcgga ggttctgttc gacggcgcca acgtgcgctt
     2041 tcgtgacttg aacagcacga acggcacgtg gttcaacggg cagcgcatcg gcgagatgag
     2101 ctggtctgcc ggcgcctcgc ttcaaattgg gcaccactac ctcacgctgc acgaagtccg
     2161 cgccgcggtg aacgcgcggg ggcgcaccgc gatcggcaac gccgctcaat acggcctcgg
     2221 ggcaccgccc gctgccgcgc cgggtccgaa cccgcacgcg ccgcagcccg gtgcgtacgg
     2281 ccagcaaccg aacccgtacg gccagcaacc gaatgcatac ggccaacagc cgaaccccta
     2341 cggccagcaa caaaacgcac ccggtcagca accggcgtat ggccaacagc cgaatcctta
     2401 cggccagcag ccaagcgcac ccggtcaaca accgaacgcc tatggccagc aggcgtacgc
     2461 ggccgccgca gccccagccg caaagaagaa gaagtcgaac gcgatctgga tcgtgcttcc
     2521 actcgtgttg gtcggcacct gcggagcggg cgcggcgggg ctctattgga tcggctcgaa
     2581 cgcgaccgaa gccgagcgcg gcggggaagc cgcgactctg ctcgatgcgc cacgcgaagc
     2641 gaccgtgaag ttcgtctggt tcaaggggcc gcccgggccg aacgcgagcg gagggacatc
     2701 gccggcgcgc gtgcgcgtgg caccgaaccg ctcgggagtc gtgtcggtgg gcgtggccga
     2761 agagttcgcg ggcggcggcg gtaaccagtg gcgcaccgcg acctggctcg cggcgttcaa
     2821 cgcgacgcgc acgctgaacg cctcgctctc cgactacgag ttcaacgtgc acgtgggcgg
     2881 gcacaccgat ggcccgtcgg cgggcatgct cacgacgacg acgatgctct cgctcttgcg
     2941 caacaagccg atgcgcgaag actcgaccat gaccggcacg atcaaccccg acggcaccgc
     3001 gggcccggtc ggcggcatcg tgcagaagat gggtggcgcg aaggagtccg ggttgaagcg
     3061 cttcggcttc cccatcggca cgcgcaacca caccgatttg aagacgcagg ctcaggtcga
     3121 cctcgtgaac gtcggtcagc agctcgggct cgagacgcgt gaaatcagcg acgtctacga
     3181 agcttacgag ttcatgaccg gcgacaagct gccgcgcgta gaaggcgtcg ccgaaaacga
     3241 gctcgagccg agtgaccaaa ctcaagcgct gttgcgggcc aaggtgagcg gctggaaggt
     3301 gcgcgtcgaa cgcgaaatcg gcgcgctcaa agaggaggtg gggcgcagcg gaaaggccgt
     3361 acaattcgcg gctccgttgc tcgctcaggc cgagcgcgcc tacgagaacg ccaagcgcta
     3421 cgagagcaac agcttcttgg tctcggcgct cgacggctac gccgaaacgg cgattttggt
     3481 cgcgtcggcc acgcgcttca cgaagatcgt gccgctcatg ttccagcgca acgtgcccgc
     3541 gctgctcgag gtcctcgagt cggccaaaac ggtgaacggc gacgtcgacg cctttgcgca
     3601 agagctcgaa gtgcgcgcgc agagcaagac ccgcggcggc caagtgagca cgacgttcgc
     3661 cttcggtcac tatgtcacgg cgcgttcggc gacgatgatc ggcgacgact ttcgcgacag
     3721 cgccgtaagg ctgatccaag gcatcgccaa cggcaacgtc gcggccgact cgaacgcgct
     3781 cgtcgccgtg ctcgaacgca cgacgatgcc catcctctac tacgatatcg cgcgcgtgta
     3841 cctcgagtac gcgaaggacg tgcaggggct catcgccgac gaaggcgatc gcaagcccct
     3901 cactccccag tcggtcgatc gcctcgtggc cggctatgcc tccgcttcgg gcgccgtgct
     3961 cgcctacttc gacgcgctca tcgtcgaaga aatcgcgaac gagaacagca tgaccgcgga
     4021 tcaagccaaa ttcgcgatgg ctcagcgcga gcccgagtac tacctcaccc tcaaatccaa
     4081 catgctctcg gagtacaccg gcaagaccgg cgcgagcgac ggggtgaagc tgatgcggct
     4141 cgccgcggcc ggcaccgcgt acctcggcgg ggccaacctc gtgaacaagt ggtactcgct
     4201 cggcatgaac ttcgacaaga gcggcaacct cgtgctcgag aaccgccgcg cgctctcggc
     4261 cgagctcgat ctggctcgcc gcaacgcgcg cgaagccgcc gcgcgagcaa aggccgcggg
     4321 tggcttcgtt cccaccgcgg cgcgcctcgc ttttcaggtc ggcagcgcgc gccgcgaggg
     4381 caacgacgag gacaagctcg cggcgctctc cgcctactgg cagtcgtcct tctggagcga
     4441 tctcgtcgcc gctcagtgag cctcgagagc gggcgcgcct acatccgttc cggcgcgccc
     4501 attcccagca agcccaagcc gagcttcaga gtttgccgcg tcgccgcgca cagcaagagg
     4561 cgtgcgtcgc gctgctctga cgtcggagcc ttcagcactg ggcagccttc gtagaacgcc
     4621 gtgtacgccg tcgcgagctg gtacaaatac gtgcacaagc gatgcggctg gagggacgtc
     4681 gccaccgcat ccaccgcccc cgcaaaatcg agcaacgcga gcaccaactc gcgctcgcgc
     4741 ttgtcctcga cgcgcgccga cacgcccggc gccgcttcgc cgcgcagcag cgcgcccgca
     4801 aatgcgaccg cgtgttcgct ctccgctttg cggaaaatcg agcagagccg cgcgtgcgcg
     4861 tactgcagat acggccccgt gttgccctcg aacgcgagca tccgatccca gtcgaagatg
     4921 tagtccttga tgcgctcgct cgagagatcg gcgtatttca gcgcgccgat gccgacgagc
     4981 tccgacacgg acgcgcgctc cgctgcgggc agctccgggt tcttgtcgcg aatcaccgcg
     5041 tcgacgcgct cgacggcctc gttcagaagt tccatcaggc gcgggctgtc accgctgcgc
     5101 gtgcgcaaca gcttcttgtc cgagcccaac accgaaccga acgcgacgtg ctcggcgcgt
     5161 acgttctccg gcaggtaacc ggcacgacgc gctgcggaaa agaccatcgc gaaatgttgt
     5221 tgttggggtg agcccacgac gtacaaaatg cgcgtcgccc cgagctcgcg agtacggaac
     5281 cgcactccgg ccaaatcggt tgcggcgtag ccgaacccgc cgtcttgctt gcgcacgatg
     5341 agcggcagcg gctccccttc ccgccccttg aattcaggcg aaaagacgca caacgcaccc
     5401 tggtcgacct gcgccacacc gatgcgctcg agctcgttcg cgacctcggc gaggaacgga
     5461 ttgtaaaagc tctccccgcg cacgtcgctg tcttccagtg tgagcccgag cttgtcgtaa
     5521 atcgtgcgga agtacttgag cgacgagtcg atcaggtgct gccacaagcg aagcgtctcc
     5581 gcctcaccgg cttgcagcgc caccacgcgc aggcgggcgc gctccgcgaa cgcttcgtcc
     5641 gcatcgaact tctggcgcgc ggccttgtag aaggcgttca ggtcgcccac ttcgagctgc
     5701 gaacgcgcct cctcggagcc gagatcgatc aggtgctcga tgagcatgcc gaagggcgtg
     5761 ccccaatccc cgatgtggtt ctggcgaatc acgcgctgac ccaagaagcc gagcacgcgc
     5821 gtcaagcagt cgccgatcac gctcgagcga aggtgcccga catgcatctc cttggccgcg
     5881 ttcggagacg gatagtcgac cactaccgtt tcgctcgttc cgctccgcgg cacgcccgcc
     5941 cgctcgtcgg tgagcagctc gctcactgcc gcctcgagga cctcgcgtcg taacgtaaag
     6001 ttcacgaacc cgggcgccga cgcctgaacg ctctcgcaaa gctccggagc ttcgatcgcg
     6061 gccgctaccg tgcgtgccac ctcgatgggc ggcttcttca aagcccgcgc gagcttcatc
     6121 gccacgtcgg cctggtagtc ggcgaaattg gagcgatgga ccgccggatc cacggatgcg
     6181 tgctcggcgc cgaagcactt cacgacagcc gccaaaacca gctcggaaag ccgagccacg
     6241 ggatggttca tggccaccgc tataactcaa ctcccgctcc cccgcccgct tactcgcgcc
     6301 tccccgtcac gctctccacc cgctcactcg cgcccgccgt cacgccccgc ccgcgcacca
     6361 gctccccatc acgctcagcc gcgctcactg tccctgtcac gtcccccgcc cgctcacggt
     6421 ccccatcatg gctcccctcc ctggatggcc catccaggcc gttccctggg ccaacattcg
     6481 cggctacact cactgcgatg cagagctccg acgtgaacgg cccgagcgac gccttctttc
     6541 gcgtttgcag cacctgcaag aagcccctcc ctttcggcga gcgctattac cgctgcagcg
     6601 tctcgacctg caaccgtccg cgcaccgcgc tctacttttg cagcgtcgcg tgctgggacg
     6661 ctcacgtgcc cgaagcgcgc caccgcgacg cctgggccga agaggtccgc gccccgaccc
     6721 gcgcggagtg gcaagcgcag caagccgaag ccgaagccga acgtcagcga agcgaggcac
     6781 cgatgaccga gaagtcaccc accgaacgtc gcatcgtcgc gcccaaagcc actggcgaag
     6841 ccgaacgcga aatcttggtc gtcgtgtcga agctcaaggc ctatatcaag gctcgcagcg
     6901 gcttcaatac ctcggacgcc gtgtccgaag tcctctcgga tcacctgcga cggctctgcg
     6961 atctcgcgat cgacaacgcc aacgtcgaca atcgcaaaac ggtgctcgac cgcgacttca
     7021 aacctttgtt caaagtcggg ccgtagcgct cgcgcagaat actcagggct tcgtgccgta
     7081 gccgagcagc gccgtgctcg aaagctcgcc ggggttctcc cgaaggtagc gttcgaggaa
     7141 ggctcgagcg cgctcggtgc cgcgctgcca ttccgattca ccgaggcgat tgcgcaggag
     7201 aacgagcggt gcactgctgc gcgacatgcg gtgccagacc tcttccgcgc tcgtgatggg
     7261 cggaacgaag cgcgtcacgg cttcgacggc gacgtcacga aagcccgccg cggtgagctc
     7321 ggccgccagc acgtcggggt tttcgaggcc gagcgggtcg cgctgcggcg gagggacgtt
     7381 cggatcgacc tcgtgaatcg cgccgaacat ccccctcatc agggacgaat ccatcagcgg
     7441 tgcccaactc gaaacgacgg cgcgcccgcc aggcttgaga acccgcagaa gttctgagta
     7501 gccccgaggg cgatcgggga agaacatcaa gccaaacatg gagaagccga aatcgaagcg
     7561 ctcgtctccg aatggcagcg actgaccatc ggcgacgcga gcggtcacgt tcgtgatgtt
     7621 acgcgtgacg agcgcggcct ggagctcttc gatcatggcc ggcgcaaagt cgatggcgtc
     7681 gatcgacgcc actcgttgcg cgagctcgag cgtcaacgtg ccgggccccg cggccacatc
     7741 gatgacgcgt gcgctcacgc ctggctccat gagctcgatg gcgcgcacgg aaaaggcacg
     7801 catcaggtac gaagcttctt cggcgtaacc tttcgacacc aaatcccacg gctctggcgc
     7861 cgccaggggc ccaccttttt gtgacatggg gcgcacggta tcacgccgcc cgacttcggc
     7921 gctttgccgc gcttccggat ttcatgaccg cgcggcggtc gcgcatcgag gtcaacgggg
     7981 cgcgggcgga acgctggtcg gcggagggct cggaacgatg ctgacggacg cttcgcgctc
     8041 tcgcacgcgc tgcacgaagc gtacgatgcc gcggccgaac aacgcgccaa ccccacccaa
     8101 aaccagggca ttaccaacga gcagagagag cgctcggtga ttcaagaggc gaccacccca
     8161 atgcatcaag tcggggagct ggtcgagatc gggccgcatc gagaagtgcg tctccatcaa
     8221 cagccgcggg ttcagccgca aatcgagcca ggcgtagaag aggatcgcgg cggtgagcac
     8281 cagccagagc cgcacgaaac tgcgcgccga atccactccg gagtctagca tgcggagcaa
     8341 acgcacccgt gcggacacgc gcgaccccgc cggtcaccgc gccgtatgcg ctcatgtagt
     8401 caattgccga agcccgatcg caacgcgacg cggcgaacac gctcacaaga acatgccggc
     8461 gacgcacgca acgagacacg tcgtcagtag tccgccgaac attgcgcgaa gcgctagacg
     8521 tgagatgtcg ccacggcgct ccggagcgag cgcgctgtaa ccaccgattt gaattccaac
     8581 gcttccgaag ttcgcgaatc cgcagagggc ataacttgcg acgactcgtc cgcggtcact
     8641 tatccacgcc ggatcttggt gaagcatttc ggccagatgc gagtacgcga tgaactcgtt
     8701 gaacagggtc ttttggccga gcaagccggc gattttggta acgtcgcctg cgggaacacc
     8761 gagcaaccaa gcgagcggag cgaacgccac cgagagaatc cgctcgagcc cgagttcgat
     8821 cccaacggca cgcccgatac cgtggagcag cgcgtcggcg agcgccgtga acgccaagaa
     8881 tacgatcagc atggctgcaa cgttcgcggc gagcttgagg ccgtcgacgg ctcccgccgc
     8941 taccgcgtcg agcgcgttgg cgtgcgcttc gggcagggcg accttcgcgt ccttgctcgt
     9001 ttcgggtgtc tccgtttcag gcaacatgag cttcgcgatg actagcgacg ccggcgcgct
     9061 catcacgctg catgcgatca ggtgccccgc gataccgggt atatcgcggc ccagcatcgc
     9121 gacgtacgca gcgaggacgc cgccggcggt cgtcgcgtag ccagcgacca tgcaggcgtt
     9181 gatctccgac gcggtcatat ttcggaggta cggtcggatc acgagcggcg cctcggtttg
     9241 cccgacgaac acgtctgcga cggtggagaa gctctccgcg ccgctcacgt tgagcgtcct
     9301 cgcgagcagt cgaccgcagc gctggatgac ccagggcatc acgcggacgt gatagagcag
     9361 gctcatcaac gccgagaaga acaggatcgt cggcagcacc tggaacgcga agatgtagcc
     9421 gaacgtcttg gggtccgcga cgagcgcccc gaacacgaag cgcgcgccgt cttgagtgaa
     9481 gccgagcagg agctcgacgg cgcgattcgc gaacgagaat aggcgcgtga gcggcgtctt
     9541 caatacgagc cccccgatcg cgagcaggag caacatcccg ccccccacgc tgcgcccgtt
     9601 cacgctgcgc cgccgctcac gggggcacag cgcgaacgcg agcccgagca tcacgagcgc
     9661 gccgagggcg ctggtgagtc gctcgagcat ctcgactcag ctcttcttgc ccagcagctc
     9721 gggcagcgga ttatctccgc ctccgaggcg cgcgatgctg tccatgtgat ggacgaggaa
     9781 ggcgcggtta tcgagcacgc tgaggttctg catctcgagg gcgccgatgc ggtcggcggg
     9841 ggtgaacgcg ggatcttcga cgcgctccat cgagagcttt tcggggccgt acgccatgta
     9901 gacggccttc gtgtcgagga tggagtagtc gtcgccgcga cggagctcga gcgtgacgga
     9961 gcccgagacg ctcggcgcga gccagcgcgt gagcgcgtct ttcagcatca tcgcctccgg
    10021 atcgaaccac ttgccctcgt agagaacgcg acccaagcgg cgaccgagcg tgaagtacag
    10081 gtcgagcgtg ttctcgttgt gcaccgccga gagcaggcgc tcccaaacga tgtggagcag
    10141 cgccattccg ggcgcttcgt aaatgccacg gctcttcgcg tcgatgacgc gattttcgat
    10201 ttggtcgctc atgccgaggc cgtgacgacc accgatgcgg ttcgcctcgc tgaagagctg
    10261 gaacaaggag tcgtagcggc gaccgttgat cgagacgggc aggccgtcgt tgaactcgac
    10321 cgtcacttct tcggtcttga tggcgacgtc cgggcgatag tgagcgacgc ccatgatcgg
    10381 ctccacgatg cgcatgccgg tgtcgagccg ctcgagatcc ttcgcctcgt gcgtcgcgcc
    10441 gagcacgttc gagtcggtgc tgtaagcctt ctcgacgctc atcgtgtgcg gcagcccgat
    10501 gctgttcagg tactcgctca tttccttgcg gccgccgaac gcgtccacga acttctgatc
    10561 gagccagggc ttgtagatct tgagctcggg gttcgtgagg atgccgtagc gataaaaccg
    10621 ctgaatatcg ttgcccttgt gggtgctgcc gtcgccgaac acgtgcacgt cgtcagcgcg
    10681 catggcgcgg atgatggcgg tcgtggtcac ggcgcggccg agcggtgtcg tgttgaagta
    10741 cttcttcccg ccactcgaga ggtggaaggc gccgcactga atcgcgacga tgccttcgtg
    10801 cacgagcgct tcgcgacaat cgatgaggcg cgcttcgacc gcgccgtgct cgagcgcgat
    10861 cttcggaatg acggagcagt cggtctcgtc gggttgggcc aggtccgcgg tgtaggagta
    10921 gaccttcatc ccattgcgag tgagccacgc gaccgcgcag cgtgtgtcga gaccaccgga
    10981 aaatgcgatg cccaggcgag tgcccgccgg gggaagcgaa cgatagatac ggctcatgac
    11041 ggccgagccg tagcacggtc ccatggccgc tgcgcaacgc ccacgtggac ccgcagaaaa
    11101 gtcgagccgt tggcggcctg ttcaccgccg ccaacgctcg gcgaaggccc ctctcgcgcg
    11161 ccgtttccaa gcttgcattc gaacttggtt ccgcgccccg gaaagggcat tccggcgcga
    11221 ttcgagaccg ctcgggcccg ttct
//
LOCUS       DASTKS010000571         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1876580, whole genome shotgun sequence.
ACCESSION   DASTKS010000571 DASTKS010000000
VERSION     DASTKS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876580"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="VFQ35_11780"
     CDS             complement(<1..1586)
                     /locus_tag="VFQ35_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931365.1"
                     /translation="MSRPPLEKHRTRRFRRVGLIVALVMLPWLLYVALGNLFLNAGGV
                     GLLLGSTREITLKYRYGWTLWPGRVNLRGFHLTFQDANVQFALDLDHANIRLALTELP
                     GRTIHATRLRGDGAAFRMRHRIQPGATHEPWVRALAPIPEFSNPPLFEATVPSPPIAE
                     SDYRLWTVHLEDVDVGISELWVQFIRYRGNARATGAFRLRPARQLWVGPAALDFNDGR
                     ITLGDDEILGAMRGRITCIAHPFDVRVPTGLAVLRHLSSKIELHGTGLRVEALPRWFS
                     THPEISLTSAPGTLDVALDVWHGRGQPGGHVSVSSDWVQAVLPRGTFRGRGFKTTSLV
                     SQDGGAESEVRIDTGEFDLKGTAARPLEFHEAALGAHIERADAADPPSGADRRIRVEK
                     VRVPDARWLNTALHLRGVEFLSGSLDGKVAFREHARRMTGTATLTLHSLRAALGSARA
                     NVDGHAELQLADASTSQATGRAQVEAQFSKAVVEAGEDAADRKGFNASLDGLGVKAKL
                     ERRKPGALEVTLSARAKSARA"
     gene            1853..3049
                     /locus_tag="VFQ35_11785"
     CDS             1853..3049
                     /locus_tag="VFQ35_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9931366.1"
                     /translation="MSLVEVATSTARAADGDDEEHKDKQSEFTAVPFAGGDSDVGFGG
                     GFIASWARLAGYDPYLLRVEAATAVTFKRPDDAWAVPYTDAYVLIDAPHVVPDRMRLR
                     LRISYTREQRLPYSGLGNASQRVPDSEDEQSRYTRIHPTADLQTELSLTKALHVLMGV
                     NYTPNQIDINPASRLAHDMNMGSDDVRRLLGDAHDHSVILFSYGVTIDTRDNLVNPRS
                     GFFATARVDLSPGESGVFTHRWARANTSVRGFVPLIPSRLTLAGRVVTDWLFGDAPFY
                     ELPRYDATSAIGGASGVRGIPAQRYFGKIKAFGNVELRSELVKMKLLGKETTWGITGF
                     FDTGRVWADYEAHPELDGTSLGLKYGTGAGLRIASGKSFVLRFDVAWSRESNPVSAYL
                     ISGHSF"
     gene            complement(3145..>3805)
                     /locus_tag="VFQ35_11790"
     CDS             complement(3145..>3805)
                     /locus_tag="VFQ35_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9931367.1"
                     /translation="LGALAAAQTVFGAATTVVVDAFVFALGFHACLVDGRRAYQWLLD
                     RSPIPRSHAVRLTRAFNETARGLLVGIGLTAVLQGAVATVGYELLSVPQAAVLGLLTT
                     AAALVPSVGTALVWLPVTAGLFLAGREEAALVLLAVGLFVSVVDNVVRPVLSRYGKLE
                     LSSFVVLVAMLGGVAAFGGFGLLLGPLFVRLALEGFRLLFEADAQSSPPPSTRANQLA
                     A"
BASE COUNT          682 a         1253 c         1259 g          611 t
ORIGIN      
        1 gctctcgcgc tcttcgctcg cgcgctcagc gtcacctcga gagccccggg ctttcgccgt
       61 tcgagcttgg ctttcacgcc gagcccatcg aggctcgcgt tgaaaccttt gcgatcggcg
      121 gcatcttctc cggcttccac cacggctttg gaaaattgtg cttcgacctg cgctcgaccc
      181 gtcgcttggc tggtcgaagc atcggcgagc tggagctcgg cgtgcccgtc cacattcgcg
      241 cgtgcgctgc ccagcgcggc acgcagcgag tgcagggtga gcgtcgcggt gccggtcatc
      301 cggcgcgcgt gctctcgaaa cgcgaccttg ccatccaggg atccgctcag aaactcgacg
      361 ccgcgtagat gaagcgcggt gttgagccag cgcgcgtcgg gaacgcgcac cttttcgacg
      421 cggatccgcc ggtcggcgcc gctcggcgga tccgcggcgt cggctcgctc gatgtgcgcg
      481 ccgagcgccg cctcgtggaa ttcgagtggc cgcgctgcgg tgcccttcag atcgaactca
      541 cccgtgtcga ttcggacttc actctcggcc ccgccatctt gcgagacgag gctcgtggtt
      601 ttgaagccgc ggccgcgaaa tgtgcctcga ggcaacaccg cctgaaccca atcagatgaa
      661 acgctgacgt ggccacccgg ctgcccgcgc ccgtgccaca cgtcgagcgc gacatccaag
      721 gtgccaggag cagaggtcag ggatatctcc gggtgcgtcg agaaccagcg cggcaaagct
      781 tcgacgcgga gccccgttcc gtggagctcg atcttgctcg agagatggcg cagcacggcg
      841 aggccggtcg gcacgcgcac gtcgaacggg tgggcaatgc acgtgattcg cccgcgcatc
      901 gccccgagga tctcgtcgtc gccgagcgtg atgcgcccgt cgttgaaatc gagcgcggca
      961 gggccaaccc acaattgtcg agcgggccgc aatcggaacg caccggtggc ccgcgcgttg
     1021 ccccgataac gaatgaactg cacccagagt tcggagatgc cgacgtccac atcctccagg
     1081 tgcaccgtcc agagtcgata gtcgctctcc gctatcggtg gcgaagggac cgtagcctcg
     1141 aacagaggag ggttcgagaa ctccgggatt ggcgccaaag cgcgcaccca gggctcatga
     1201 gtcgcgccgg gctggatgcg atgacgcatg cggaaagccg cgccatcgcc gcggaggcgg
     1261 gtcgcgtgga tggtgcgccc gggaagctcg gtgagcgcga gtcgaatgtt ggcgtggtcc
     1321 aagtcgagcg cgaactggac gttggcgtct tggaacgtga gatggaagcc gcgcaagttc
     1381 acccgtccgg gccatagcgt ccatccgtag cggtacttca gcgtgatttc gcgcgtcgaa
     1441 ccgagcaaca acccaacgcc gccagcgttg aggaacaggt tgccgagcgc gacgtagagc
     1501 agccaaggca gcatcacgag cgcgacgatg agcccaacac gccgaaacct ccgagtccgg
     1561 tgcttttcga gaggaggcct cgacactcgc acgtcgcgat gagcagcacc tttcgtgcca
     1621 ctgccatgcg tggcggatgc gccgccgaaa tggtgcgagc ccgagcgcgg cgaggcaaag
     1681 tgccatgctc ggtcgcctca ctgaggcgcg gtaccgaacc aatgagcgaa aacgctcgcg
     1741 caaccgtcgc cgacttgctt ggcgcgggag ttgcccatcg ggcgagctgt tggcatttac
     1801 caaaccacgt ccgtcgcgcg ttgtgcgtcc tgcgttgtgc gcgctgtggc tggtgagcct
     1861 cgtcgaagtg gcgacatcga ccgcgcgagc agcggacggg gacgacgagg agcacaagga
     1921 caaacagtcc gaatttacgg ccgtgccctt cgccggcggc gacagtgacg tcgggttcgg
     1981 cggtggcttc atcgcgtcgt gggcccggct cgcgggctac gacccgtatc tattgcgcgt
     2041 cgaggcggcg acggcggtca cgttcaagcg gccggacgat gcgtgggcgg ttccgtatac
     2101 cgacgcttac gttttgatcg acgcgcctca cgtcgttccc gatcgcatgc ggttgcggct
     2161 tcgaatttcg tatacgcgcg agcagcgact gccttactcc ggactcggca acgcgtcgca
     2221 gcgggtgccc gattcggagg acgagcagag ccgctatacc cgcattcacc cgacagcaga
     2281 cctccagacg gagctcagct tgaccaaagc gctccacgtc ttgatggggg tgaactacac
     2341 cccgaaccag atcgacatca accccgcgag ccgcctggcc cacgacatga acatgggctc
     2401 ggacgacgtt cgccgactct tgggcgacgc gcacgatcac tccgtgatcc tcttttctta
     2461 tggcgtcacg atcgatacgc gcgacaactt ggtgaacccg cggagcggct tcttcgcgac
     2521 cgctcgcgtc gacctgtcgc ccggcgaaag tggcgtattc acccatcgtt gggcccgcgc
     2581 gaatacgagt gtgcgcggct tcgtgccgct catcccttct cggctgactt tggcggggcg
     2641 agtcgtcacg gattggcttt tcggggacgc ccccttctac gagctgcctc gctacgacgc
     2701 gacttcggcg attggaggag cgagcggtgt ccgcggcatc cctgcccagc gctatttcgg
     2761 caagatcaag gcgttcggca acgtggagtt acggagcgag ctcgtgaaga tgaagctgct
     2821 cggcaaagag acgacgtggg gaatcaccgg cttcttcgat acggggcgag tctgggcgga
     2881 ctacgaagcc catccggagc tcgacggaac gtcgctcggg ctgaaatacg gtaccggggc
     2941 tgggctccgc atcgcttctg gcaagtcgtt cgttctgcgc ttcgacgtcg cctggtcgcg
     3001 cgaatcgaac ccggtctcgg cctacttgat ctcggggcac tctttctgag cgaactccac
     3061 tggctcgacg agaacctgcg cgccgaacag cccatcgcag ctcgcccgac cccgcggcgt
     3121 ggcgtatcgc cagccgtaga cagactacgc cgcgagctgg ttcgcgcgcg tcgacggagg
     3181 cggagagctc tgcgcgtccg cctcgaacag gaggcggaag ccttcgagcg ccagacggac
     3241 gaagagtggg cccaagagca ggccgaaacc accgaatgcc gcgacgccgc cgagcatcgc
     3301 tacgagcacc acgaagctcg agagctcgag cttgccgtag cgcgaaagca ccggtcgaac
     3361 gacgttgtcg accacggaga cgaatagccc gactgccaag agcacgaggg cagcttcttc
     3421 gcgccctgcg aggaagagcc ccgccgtgac cggcagccaa acgagcgcgg taccgacgga
     3481 gggaaccagc gccgccgcgg tcgtgagcag gccgagcacg gccgcctgcg gtaccgagag
     3541 cagctcgtac cccacggtcg ctaccgcgcc ctgcagcacg gcggtgagcc cgatccccac
     3601 caagaggccg cgcgctgtct cgttgaaggc gcgggtgagg cgaaccgcgt gcgagcgcgg
     3661 aatcggactg cgatcgagca gccactggta tgcgcgccgc ccgtcgacca aacaagcgtg
     3721 aaagccgaga gcaaacacga aggcgtccac caccaccgtc gtcgcggcgc cgaacacggt
     3781 ttgagccgcc gcgagcgcgc cgagc
//
LOCUS       DASTKS010000572         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_538119, whole genome shotgun sequence.
ACCESSION   DASTKS010000572 DASTKS010000000
VERSION     DASTKS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538119"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFQ35_11795"
     CDS             complement(<1..657)
                     /locus_tag="VFQ35_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9931368.1"
                     /translation="MNNEATLANPRTMRARYTKARVAVVEGPDAGSFIDMASQAVRIG
                     TSSQNDLVLTDQTVSRRHCALVPVPEGLRLVDEGSRNGVLVGAMRVFEAVLQGAQRLT
                     IGQSVLSVVPLEETVEREQITDDRFGGLLGGSAQMRELYADLERIAASDISVLIEGET
                     GTGKELVAESIHAKSGRAERPFVVFDCSAVVPTLAESELFGHERGAFTGAVATRVGVF
                     E"
     gene            835..1527
                     /locus_tag="VFQ35_11800"
     CDS             835..1527
                     /locus_tag="VFQ35_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931369.1"
                     /translation="MPSTSPRVFRTSPLVVMFASATTAACLGLGCETAVQEQSYELTL
                     RVLSDPNEPLAGANASRGSAALGVSDEHGQVHVLARGREGERVAIDVACPDGYRSPEG
                     PIAVTLRKVAGGHGPEYEVRCPPLRRQLVIAVRAERGANLPVRYLNREIGRTDEAGAA
                     LVMLESFTDDTLELTLDTSEEPRLLPRSPSTRFRMPDHDEIVVLNQRFELEPKAIQRV
                     HRRKSAGPIRIQ"
     gene            1524..1694
                     /locus_tag="VFQ35_11805"
     CDS             1524..1694
                     /locus_tag="VFQ35_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931370.1"
                     /translation="MTNRSLASETRGSVFVEYIVLVVFVGLVVAIALAKEAPRTLLMY
                     SSQRAALYQPNP"
     gene            1739..1912
                     /locus_tag="VFQ35_11810"
     CDS             1739..1912
                     /locus_tag="VFQ35_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931371.1"
                     /translation="MKSLLRKLAHDTRGAGFVEYIILVGVVALLCVAAFRTFGTDVGD
                     KIKSQGTEVKGIK"
     gene            2017..2583
                     /locus_tag="VFQ35_11815"
     CDS             2017..2583
                     /locus_tag="VFQ35_11815"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9931372.1"
                     /translation="MTPTQVVLLGLLATTATASYFDVRTRHIPNRMLVATGSVGVALH
                     FGAYAVLAHEAGGDWLAAAGSAAANIAAGVLVCAFVPVLLFRFGAMGGGDVKLLAVVG
                     ALSGPVLGAQIELYAFLLAALYAPLRLAFQGQLLRLLGNSAVLVTNVFLPKERRRPLP
                     TELLSELCFAPSVLASTVLVAFLRWGSR"
     gene            2580..3065
                     /locus_tag="VFQ35_11820"
     CDS             2580..3065
                     /locus_tag="VFQ35_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931373.1"
                     /translation="MKPWAALSESTRGAVFVEHIIAFLPVMFFFLATWQLIELCAAQL
                     MVKRAASAAARAAVVVLTDDYHFYSDTPVNTFAGERKRDIELAAAMILATSPHFSSDI
                     TVSVSSATGSAPLTATVKAKFHCIAGWVSVVCGGAYRELEAKSTYAYQGADYDYDMGG
                     L"
     gene            3065..4765
                     /locus_tag="VFQ35_11825"
     CDS             3065..4765
                     /locus_tag="VFQ35_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931374.1"
                     /translation="MREHDASLKSDTRGAIMIVGIVAGAILVGALWHIASVGDAILWR
                     ERAQDAADAAVFENAVWHARGMNMCAAINIVMAAVLSILVIWRTILILVSIALVIATI
                     LCVVTLGTGCGFAGAVLRVETFMLRNDNKVANGVVRVLGAMNAAEVAVATATPLVALG
                     TAKVHTEQAYVVNSAWTQSMSLLPSINDKGFQQLQRCAKGWKAGTAVKKKPKPGSKAD
                     YYGKFNDYINQSRMGFVVSLPVQLETYGLLCDKAGQFVLNSLAGILESMGVPAGAIEG
                     IDKAKVALGGIVSMLPGLFCAPMGTPPGVQKMIDDQAAESCRSDKDKDRVFVAGGPDG
                     TEVKYKDSSGKLVSEDDYVKDCSKKKKKDARNKLDDAFKQTGDQYGFAECGQPSKVWE
                     WAVNGNVFMRSFSEVKKDTPMLTRDDKGLMVGAFQGAKLEAVDTEDILAHAEIYYDCK
                     GPWASGTCRSNAMWALRWKARLRRVQSVGKLVSTALEPIIVSTLTQAINELANEKVND
                     FVKKKFKVPPILFPKLKETWIARYVKTNLVMGGLYGSEGFEKVGNLVERASADSGVIH
                     "
     gene            4777..>5145
                     /locus_tag="VFQ35_11830"
     CDS             4777..>5145
                     /locus_tag="VFQ35_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931375.1"
                     /translation="MSPHSTSAVKRKPRRLRLPNPRGAVFVEAIIMCTLFGLIMAGAV
                     FFHRLYFTKIKVVEEARAAVWTQAMGGCNAAADLAAIWSTGSNTAGGEIDTDSTPSFF
                     GSISHTDGSAARTVAKPSIIG"
BASE COUNT          971 a         1642 c         1626 g          906 t
ORIGIN      
        1 ttcgaagaca ccgactcggg tcgcgaccgc gccggtaaac gcgccccgct cgtgaccaaa
       61 gagttcgctt tcggccaaag tcggtacgac cgcgctgcag tcgaagacga cgaagggcct
      121 ttcggcccga ccgctcttcg catggatcga ctcggcgacg agctcttttc cggtcccggt
      181 ctcgccctcg atgagcacgc taatgtccga agccgcgatg cgctccagat cggcgtagag
      241 ctctcgcatc tgcgccgagc cgccgagcaa gcccccaaat cggtcgtccg tgatttgctc
      301 tcgctcgacc gtctcttcga gcggcacgac cgacaagacg ctttgcccga tggtcaggcg
      361 ctgagcccct tgaagcaccg cctcgaacac gcgcatcgcg ccgacgagaa ctccgttcct
      421 cgaaccctcg tccacgagtc gcaacccttc cggcacgggt acgagggcgc agtgccgccg
      481 cgaaaccgtt tggtccgtga gcaccaggtc gttctgtgac gacgtgccga tgcgaacggc
      541 ctggctcgcc atatcgatga aagatcccgc gtcgggccct tcgacgaccg cgacgcgcgc
      601 cttcgtatag cgagcacgca tcgtcctcgg gttcgctagc gtcgcttcgt tgttcatcgc
      661 gcaggagctc gactccgaat gttgcgcgag tccgtcacgc gatcaagcag tggaaactca
      721 gagtgacaac tagaggttgt cacttcctcg tgggtttacg gtaaacgaat cgccacatat
      781 gcattattca gcgggcgaac gcggcggcac agcgcgtgca ctggtcgcgc cgcgatgccg
      841 agcacgagcc cgcgtgtgtt ccgtacgtca cccctggtgg tgatgttcgc cagtgcgacg
      901 accgccgcgt gcctcgggct gggctgtgag accgcggttc aggagcagtc ctatgaattg
      961 acgctgcgcg tcctttcgga cccgaacgag ccattagccg gagcgaacgc gagccgtggc
     1021 agcgcggcgc tcggagtaag cgacgaacac ggccaggtcc acgtgctcgc ccgcggtcga
     1081 gagggtgagc gcgtcgcgat cgacgtcgcg tgcccagatg gctaccgctc gccggaaggc
     1141 cccatcgcag tcactttgcg caaagtggcc ggcgggcacg gccccgagta cgaggtgcgc
     1201 tgcccgccac tcaggcgcca gttggtgatt gcggtgcgcg ccgagcgcgg agccaacctg
     1261 cccgttcgct acttgaaccg cgaaatcggg cgcaccgacg aagcgggcgc cgcgctcgtc
     1321 atgctcgaga gcttcaccga cgacacgctc gagctcacgc tcgacacctc cgaagagccg
     1381 cggcttctgc cgcgcagccc ttcgacccgc ttccgaatgc ctgatcacga cgagatcgtc
     1441 gtcctgaacc agcgcttcga gcttgagccc aaggccattc aacgcgtgca ccgtcgcaag
     1501 agcgcggggc caatccgtat ccaatgacaa accgaagctt agcgtccgaa acccgcggct
     1561 ccgtcttcgt cgaatacatc gtgctcgtcg tgttcgtcgg cctcgtcgtg gcaatcgcgc
     1621 tcgccaaaga ggccccgcga acgctgctca tgtactcgag ccaacgcgcg gcgctctacc
     1681 aaccgaaccc ctgagtcgac tcgacctcgt aacgaaccca gaaagaaagg cacactcaat
     1741 gaaatcgttg cttcgtaaac tcgcacacga cacccgcggc gccggcttcg tcgaatacat
     1801 cattttggtg ggcgtagttg cgctcttgtg cgtagccgcg ttccgcacgt tcggcaccga
     1861 cgtgggcgac aagatcaaga gccagggcac cgaagtcaaa ggcatcaagt agtttcgctc
     1921 gcgcacgcga ttcgactgaa cgaccctgca ggaggcctgt tcatcgaacg cgcaggcccg
     1981 cggcaccaac gcgctccccg actcctggac cctatcatga cgccaactca agtcgtgctt
     2041 ttgggattgc tcgcaaccac ggccacggcg agctacttcg acgtacgcac ccgccacatc
     2101 cccaatcgta tgctagtcgc caccggcagc gttggcgtcg cgctgcactt cggagcttac
     2161 gccgtgctgg cgcacgaagc gggcggagat tggctcgcgg cggcggggag cgcagccgcc
     2221 aacatcgcgg ccggcgtatt ggtctgtgcg ttcgtgccgg tgctcttgtt tcgattcggc
     2281 gcaatggggg gcggcgacgt gaagctgctc gcggtggtcg gcgcgctctc gggaccggtg
     2341 ctgggggcgc aaatcgagct ctatgcgttt ttgctcgcag cgctttatgc gcccctccgg
     2401 ctggctttcc aagggcagct cctgcggttg ctagggaact ccgccgtgct ggtcaccaac
     2461 gtgttcttgc ccaaggagcg gcggcgcccg ctgcccaccg agcttttgag cgagctctgc
     2521 ttcgcgccgt cggtgttggc gagcaccgtg ctcgtggctt tcctccgctg gggctcgcga
     2581 tgaagccctg ggccgcgctc tccgagagca cgcgcggggc cgtgttcgtc gaacacatca
     2641 tcgccttctt gccggtaatg ttcttctttc tcgcgacctg gcagctcatc gagctctgcg
     2701 cggcgcagct gatggtgaaa cgcgcggcgt cggcagcggc gcgcgcggcg gtggtagtgc
     2761 tgactgacga ctaccacttt tactccgaca caccggtgaa cacgttcgct ggcgagcgca
     2821 agcgcgacat cgagcttgcc gccgccatga tcctcgcgac ttctccgcac ttctcgagcg
     2881 acatcacggt gagcgtcagc tcggcgactg ggtcggcgcc gctgacggct acggtcaaag
     2941 cgaagttcca ctgcatcgcg ggctgggtca gcgtcgtctg tggtggcgct taccgcgagc
     3001 tcgaagccaa atcgacctac gcctaccagg gcgccgacta cgactacgac atgggagggc
     3061 tctgatgcgt gagcacgatg cttctctgaa gagcgacacg cgaggcgcaa tcatgatcgt
     3121 cggcatcgtc gccggcgcga tcctggtcgg cgcgctctgg cacatcgcga gcgtcggaga
     3181 tgccatcttg tggcgcgaac gagcccaaga cgccgccgac gcggccgtgt tcgagaacgc
     3241 ggtttggcac gcccgcggca tgaacatgtg cgcggcgatc aacatcgtca tggcggccgt
     3301 gctctcgata ttggtcatct ggcggacgat cttgatcttg gtatcgatcg cgctcgtcat
     3361 cgctacgatt ctctgcgtcg tcacgctggg gacggggtgc gggttcgcgg gcgcggtgct
     3421 gagggtcgaa acgttcatgc tgcgcaacga caacaaagtc gcgaacggcg tcgtgcgagt
     3481 gctcggcgcc atgaacgcgg ccgaggtcgc ggtcgcgacc gcaacgccac tcgtcgcgct
     3541 cggcaccgcc aaagtgcaca ccgagcaagc ttacgtcgtc aatagcgcgt ggacgcaaag
     3601 catgtcgctg ttgccctcga tcaacgacaa agggtttcaa cagctgcaac gctgcgcaaa
     3661 gggctggaag gcggggaccg cggtgaagaa gaagcccaaa ccggggagca aggcggacta
     3721 ctacggcaaa ttcaacgact acatcaatca gtcgcgcatg ggcttcgtcg tgagcttgcc
     3781 cgtccagctc gagacttatg ggctcctttg cgacaaggcg ggtcaattcg tgttgaacag
     3841 cttggcgggg atcctcgaga gtatgggggt accggccggc gccatcgaag gcatcgacaa
     3901 agcgaaggtc gcgctaggcg gcatcgtcag tatgctgccc gggctcttct gcgctccgat
     3961 gggcacaccg cccggagtgc agaagatgat cgacgaccag gccgcggagt cttgccggag
     4021 cgacaaggac aaggaccgcg tcttcgtcgc cggggggccc gacgggaccg aagtcaaata
     4081 caaggacagc agcggaaaac tcgtgagcga agacgactac gtaaaagact gctcgaaaaa
     4141 gaagaagaag gacgcacgca acaagctcga cgacgccttc aagcagaccg gtgaccagta
     4201 cgggttcgcc gagtgcggac agccttcgaa agtctgggaa tgggccgtga acggcaatgt
     4261 gttcatgcgg agcttttccg aggtgaagaa ggacacgccc atgctcacgc gcgacgacaa
     4321 gggcttgatg gtgggcgcgt ttcaaggcgc caagctcgag gcggtggaca cagaagacat
     4381 ccttgcgcac gcggaaatct attacgactg caaggggcct tgggcatcgg gaacctgtcg
     4441 ctccaacgcg atgtgggccc ttcgctggaa ggcgcgcctg cgccgcgtgc agtcggtggg
     4501 caagctcgtc tcgacggcgc tcgagcccat catcgtgtcg acgctcacgc aggccatcaa
     4561 cgagctcgcg aacgagaaag tgaacgactt cgtgaagaag aagttcaagg tcccaccaat
     4621 cctattcccc aagctgaaag agacgtggat cgcccgctac gtgaagacga acctcgtgat
     4681 gggaggcctt tacgggagtg aaggcttcga gaaagtcggc aatctagtcg agcgcgcttc
     4741 cgccgactcc ggcgtcatcc attgatggtg agcacgatgt cgccgcattc cacctccgca
     4801 gtcaaacgca agccgcgacg cctacgcctg cccaatccgc gtggcgccgt gttcgtcgaa
     4861 gccatcatca tgtgtacgct cttcgggctg atcatggcgg gggcggtgtt ctttcaccgg
     4921 ctctatttca cgaaaatcaa ggtagtcgaa gaggcgcgag ccgcggtgtg gacccaagcg
     4981 atgggcggct gcaacgccgc ggcggatctc gctgcgatct ggtcgacggg ctcgaacacg
     5041 gcgggagggg agatcgacac cgactcgact ccgagcttct ttggcagcat ctcgcacacg
     5101 gacggcagcg cggctcgcac cgtcgcaaag cccagcatca tcggc
//
LOCUS       DASTKS010000573         5515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_91984,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000573 DASTKS010000000
VERSION     DASTKS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91984"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFQ35_11835"
     CDS             <1..943
                     /locus_tag="VFQ35_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9931376.1"
                     /translation="LWFVLRYGVGGFYVVSPNERAVLTTFGRAQRLGEGTTLDDPLSA
                     SLKPEERERYVYPLVRAIGPGFYWKLPWQSVHKVSVATQTASIAFDPELPSANNNGQM
                     LEAVTKDQLNIGLTGQIRFTISERNLYAYLFGVRNPIAHVMGYFISVLRDRIANFEAP
                     DASRNPSDVVAAQAISINDLRKHLRNLNERMNVECVSAEARYGMVLDAALITGIDSPA
                     EVESALAAINTAYNQVSSEVSLAQAAADQKIVQSRRAVEIETLNAQAEVEPLRALAEQ
                     LWTLKQAGPGVLGAYIRNAKLVLFRRAKQLVLSREGV"
     gene            943..1833
                     /locus_tag="VFQ35_11840"
     CDS             943..1833
                     /locus_tag="VFQ35_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9931377.1"
                     /translation="MEFLISVFVTALLCFIAVPVLLGLGSAFCLYVVVRERQAVVFEL
                     FGRVRAVISEPGLSSPWLHMGPVAMLVPIFGKRRVVDLRLGQTYLRSQAVNSEEGAPM
                     GIGVWYEMYVSDPLAYLYKNTDPQGSLLANVSNATVRSLSNMKLERMFEERHSMSRSV
                     REEVSPHSQEWGYRIGSVYIRKVHFRDPGMVRQIEQKVVNRLRQVTAAIQQDGANRVN
                     VIHSAAEREAAIEFARAAATRPRIVGEAIATIARDAEICGALFELLETQQLTAAEGLS
                     ISFVPEGARLLPELLAARGR"
     gene            1936..2397
                     /gene="rpiB"
                     /locus_tag="VFQ35_11845"
     CDS             1936..2397
                     /gene="rpiB"
                     /locus_tag="VFQ35_11845"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9931378.1"
                     /translation="MPELTPLRIALGSDHAGFPLKERVKTFLQELGHEVVDFGCFNTE
                     RCDYPDFCRPAAEAVRSGDCQRCIVFGGSGNGEAIVANRVPGVRAPLCWNLETARLGR
                     EHNDANGLALGARVIDHELALEIVRVWLNTPFEGGRHVPRIRKIDDPGYRS"
     gene            complement(2429..3256)
                     /locus_tag="VFQ35_11850"
     CDS             complement(2429..3256)
                     /locus_tag="VFQ35_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931379.1"
                     /translation="MTTLTQADVLRFLQTPPTFPRLDTPLKQAVFSFTLASGYIFANQ
                     LEPHVAEALERSGKISSVDPVATLLYDFGARVVRPLAEKLLTPNEYAAVHSYVQDMTA
                     LANEPSGDGSSWKDLATALNKLPTLGDLGIRIMTVRGVSKEYAQKFDSKITGLIWNSS
                     DGALYLQIGNQVHKHGLGHFTSTSLFRNASFWALLSDKDVGFLVVDDTGVPIGWSSSY
                     LFFDGSEVLYRPGSVTHLQRLGRSSLQHTVLYLCERERIDPGQRYLVNDTTLVSQSF"
     gene            complement(3556..4842)
                     /locus_tag="VFQ35_11855"
     CDS             complement(3556..4842)
                     /locus_tag="VFQ35_11855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET9931380.1"
                     /translation="MKSKRAFIIVDLGFGDAGKGLLTDYLVRRENADLVVRFNGGAQA
                     GHNVVTADGRHHTFSQFGSGTFVPGVRTHLGRDVVIHPTALAVEAQALAKAGETRALE
                     RLSVDPECRVTTPFQQAHNRLLELARGAHRHGSCGVGFGPTVDDSISRPELVVRFGEL
                     VRPTNALRERLRHEREHKLSQWPHISPEQEREFVALKNPDVLERWLGAASHVANQVTL
                     LESGAAIRGAERIVFEGAQGVLLDQDCGFHPYTTYSRCTPHAARALLHAAAFEGSAHC
                     VGVLRSYMVRHGPGPLPTEAAELVRVTAEPHNRHDPWQGAVRKGYFDVPLLRYALAAS
                     ASVDALCITHLDALPERICARYDSVTTLPLPRNLAEQERLTELLLRQRPVLSPVGSER
                     AFLELVGASTDVPVEYTSTGPRASDVHPWSPTRLGA"
     gene            complement(5253..>5515)
                     /locus_tag="VFQ35_11860"
     CDS             complement(5253..>5515)
                     /locus_tag="VFQ35_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931381.1"
                     /translation="ICLESPEHTCWAAAGIIALSEGAVPDLPAFAERLIALGLDRAKV
                     GAITRALVPYAASIDRAGAATPRTEADDATSPKPSFWKRLFG"
BASE COUNT          925 a         1681 c         1891 g         1018 t
ORIGIN      
        1 tctctggttc gtgctgcgct atggcgtcgg cggcttctac gtggtcagcc cgaacgagcg
       61 cgccgtgctc accacgttcg ggcgcgcgca gcgcctcggc gagggaacca ccttggatga
      121 ccccctttcc gcgagtttga agccggaaga gcgcgaacgg tacgtctacc cactggtgcg
      181 cgcgatcggc cccggcttct actggaagct cccctggcag agcgtgcaca aggtgtcggt
      241 ggcaacccag accgcgtcga tcgccttcga tccagagctc ccttccgcca acaacaatgg
      301 gcaaatgctc gaggcggtga cgaaggatca gctcaacatc ggcctcaccg gacagattcg
      361 cttcacgatt tcggagcgca acctctacgc ttacctcttc ggtgtgcgga accccatcgc
      421 gcacgtgatg ggctacttca tttcggtttt gcgcgaccgc atcgccaact tcgaagcgcc
      481 cgacgcttcg cgcaacccga gcgacgtcgt ggccgcgcag gcgatttcga tcaacgattt
      541 acgcaaacac ctccgcaact tgaacgagcg catgaacgtc gaatgcgtct cggcggaggc
      601 gcgctacgga atggtcctcg acgccgcgct catcaccggg atcgactcac cggcggaggt
      661 agaaagtgct ctcgccgcca tcaacaccgc ttacaaccag gtctcgagcg aagtgagcct
      721 ggcccaggcc gccgccgacc agaagatcgt gcagtcgcgt cgcgcggtcg aaatcgagac
      781 cctcaacgct caggcggagg tggagccgtt gcgtgcgctc gcggagcagc tttggacctt
      841 gaaacaagca ggtccggggg tcctcggggc ctacatccgc aacgcgaagc tcgtgctgtt
      901 tcggcgggcc aagcagctag tgctttcgcg ggagggcgtg tgatggagtt tttgatcagc
      961 gtgttcgtga cggccttgct gtgcttcatc gcggtgccgg tgctgctcgg gctcggcagc
     1021 gcgttctgcc tttacgtcgt ggtgcgcgag cgacaggcgg tagtgttcga gctcttcggt
     1081 cgggttcgcg ccgtcatctc cgagcccggg ctctcgtcgc cgtggcttca catgggccca
     1141 gtcgccatgc tcgtgccgat cttcggaaag cgccgcgtgg tcgacctccg cttgggccaa
     1201 acgtaccttc gcagccaagc cgtgaactcc gaagagggcg cgccgatggg gatcggcgtt
     1261 tggtacgaga tgtacgtgag cgatcccctc gcctacctct acaagaacac ggatccgcaa
     1321 ggttcgctcc tcgccaacgt ctccaacgcg accgtgcgca gcctgagcaa catgaagctc
     1381 gagcgcatgt tcgaggagcg gcactcgatg agccgcagcg tgcgggaaga ggtttctccg
     1441 cactcacaag agtgggggta ccgcattggc agtgtgtaca tccgaaaggt gcactttcgc
     1501 gaccccggta tggtgcgaca gatcgaacaa aaggtcgtca atcgcctccg ccaggtgacc
     1561 gctgcgattc agcaagacgg cgcgaatcgc gtcaacgtga ttcactctgc cgccgaacgc
     1621 gaagccgcga tcgagttcgc tcgcgccgcc gcgacgcgcc cgcgaatcgt cggcgaagcc
     1681 atcgccacca tcgcgcgcga cgccgaaatc tgcggtgcgc tcttcgagct gctcgagacg
     1741 cagcaactca ccgccgccga aggcttgagc atctcgttcg tccccgaagg cgcccggctc
     1801 ttaccggagc ttttggcggc gcgggggcgc tgagccctcg cggttcgggc tgagcgctca
     1861 cggtaggttc gcgctgaggg ctcgcggtgc gcgccgtgct ttgttcgcca gactccgtgc
     1921 tagcgtggcc gccgcatgcc tgaactcacg cccctgcgca tcgctctcgg ctcggaccac
     1981 gctggcttcc ccctcaagga gcgggtcaaa acctttctcc aggagctggg tcacgaggtc
     2041 gtagatttcg gctgtttcaa tacggagcgg tgtgattacc ctgacttttg ccgccccgcg
     2101 gcggaggcgg tgcgtagcgg cgattgccag cgctgcatcg ttttcggtgg ttcgggtaac
     2161 ggcgaggcga ttgtcgcgaa ccgtgtgccg ggggttcgtg cgccgctgtg ctggaacctg
     2221 gagacggctc gtttagggcg cgagcacaac gacgccaacg gcctcgctct cggcgcgcgg
     2281 gtgatcgacc acgagctagc gctcgagatc gtgcgagtct ggttgaatac gccgttcgaa
     2341 ggcgggcgcc atgtgccacg gatccgcaag atcgacgatc cgggctaccg gtcctgaccg
     2401 tcgatcactc gctggctccc cccgcctctc agaagctctg cgacacgagc gtcgtgtcgt
     2461 tgacgaggta tcgttggccc gggtcgatgc gttctcgctc gcacaggtag agcaccgtgt
     2521 gctgtaggct cgagcgcccg agccgttgca ggtgcgtgac cgaccccggt cgatacagca
     2581 cctcggaacc gtcgaaaaac aagtagctcg acgaccatcc gatcggaacg ccggtgtcat
     2641 ccacgacgag aaagccgacg tccttatcgg acaagagagc ccagaacgag gcattgcgaa
     2701 acagcgacgt cgaggtgaaa tggccgaggc cgtgcttgtg cacctgattg ccgatctgaa
     2761 ggtagagcgc gccatccgag ctgttccaaa ttagcccggt gatcttcgaa tcgaactttt
     2821 gcgcgtattc cttcgaaacc ccgcgcaccg tcatgatccg gatgccaagg tcacccaacg
     2881 tgggtagctt gttgagcgcc gtcgccaagt ctttccagga agaaccgtca ccggagggct
     2941 cgtttgcgag cgcggtcatg tcctgcacat agctgtgcac cgccgcgtac tcgttggggg
     3001 tcagcaactt ctcggcgagg gggcgaacga ctctcgctcc gaagtcgtag aggagggtcg
     3061 ccacgggatc cacggagctt atcttcccgc tgcgttccag ggcctccgcg acgtgaggct
     3121 cgagttggtt ggcgaagatg tatccagacg cgagcgtaaa gctgaatacg gcctgtttga
     3181 gcggagtgtc gagtcgagga aacgtcggcg gggtctgaag aaaacgcaga acatccgctt
     3241 gcgtcagagt cgtcacccat ttcgtcccgt cgtggtaccg aaagtactgg tgccacagct
     3301 gaaccagata tctctcatcg aacggcaact ttcgacatcc ccgttggtgc cgggtcgttc
     3361 aaccatcgtt gcgcgacgag cggcgagtcc gagcatattc tcaagagcac atctttcaag
     3421 atggtttggc cggtacgaga cccggaacac ggtgcgtagg ggtgcggacg cggcgtaatg
     3481 cgccgtcgta gcgttaggcg tggcgagcgc agctcttgga agtgagcccg tgacggcgcc
     3541 ccgctcaggg ggaagctatg cgccgagcct ggtcggtgac cacgggtgca cgtcgctcgc
     3601 cctcggtccg gtcgacgtgt actcgacggg gacgtcggtg ctcgctccga cgagctcgag
     3661 gaaggcgcgc tccgagccca ccggcgagag aactggccgc tgacggagca agagttccgt
     3721 gagtcgttcc tgctcggcga gattgcgggg cagcggaagc gtggtcacgg agtcgtagcg
     3781 cgcgcaaatg cgttccggca gcgcatccag gtgcgtgata cagagcgcgt cgacgctggc
     3841 gctcgcggcg agcgcgtagc ggagcagcgg cacgtcgaag tagcctttgc gcacggcgcc
     3901 ttgccaaggg tcgtgacgat tgtgcggttc ggcggtgacg cggacgagct cggcggcttc
     3961 ggtcggcagc gggccggggc cgtgacgcac catgtagctt cgcagcacgc cgacacagtg
     4021 cgcagagcct tcgaaggcgg cagcatgaag taacgcgcgc gccgcgtgcg gggtgcagcg
     4081 gctgtacgtc gtgtagggat ggaagccgca atcctgatcg agcaagactc cttgcgcgcc
     4141 ttcgaagacg atgcgctctg ctccgcgaat ggccgcgccg ctctcaagca gcgtcacttg
     4201 gtttgcaaca tggctcgctg cgccgagcca gcgctcgagc acatcggggt tcttcaacgc
     4261 gacgaactcg cgttcttgct ccggcgagat gtgcggccat tgagagagct tgtgctcgcg
     4321 ttcgtggcgt agacgctcgc gcagcgcgtt cgtagggcgt acgagctctc cgaagcgcac
     4381 gacgagctcg ggacgagaga tggagtcgtc caccgtgggc ccaaacccaa ccccacaact
     4441 tccatgacga tgggcgccgc gtgcgagctc tagcaagcgg ttgtgcgctt gttggaacgg
     4501 cgtggtgacg cgacattccg gatcgacgct gagtcgctcg agggcgcgcg tttcgcctgc
     4561 cttcgctagc gcctgtgctt cgaccgcgag cgcggtgggg tggatgacga cgtcgcggcc
     4621 gaggtgggtg cgcacgccgg gcacgaatgt gccgctgccg aactgcgaaa aggtgtggtg
     4681 tcgcccatcg gcggtcacga cgttgtggcc cgcttgggcg ccgccgttga agcgcacgac
     4741 gaggtcggcg ttttcccgcc gcacgaggta gtcggtgaga aggcccttgc cggcgtcgcc
     4801 gaagcccaaa tcaactatga tgaaggctct tttgctcttc atttagtctc ctgccaaccg
     4861 gtcgccgtgt gctccgtcgg ggccgcgcgg ggcccttccc cgctgggtgc gcttcgcgat
     4921 gctgccatga cctgagaggt tggttcgagg ctcgagaggt tgtggggcgg gggccttccc
     4981 acgcggcccc gacggagcac acggctccct ctgtgtggac tgacgcgatg gctacgaggc
     5041 gaacggctct cggcggattg gagcagtgat tcggcgcggc tccttcggag cgcgggtgtc
     5101 cgcggaggga gcggtgattc cgcgcggctc cttcggagcg cgcggggcga tgtgcgagcg
     5161 ggagagtcgc gcggctcctt cggagcgcgc gggcggtgtg cgagggggag gggcttgagg
     5221 gatgcgagga tggtgatgtt ggttggtggc gatcagccga atagtcgttt ccagaatgac
     5281 ggtttcgggc tggtggcgtc gtccgcttcg gttcgggggg tggcggctcc ggcgcggtcg
     5341 atgctggcgg cgtaggggac gagggcgcgg gtgatggcgc cgactttggc gcggtcgagc
     5401 ccgagggcga tgaggcgttc ggcgaaggcg gggaggtcgg ggactgcgcc ttcgctgagc
     5461 gcgatgatgc cagctgcggc ccaacaggtg tgttcgggcg attcgaggca gatga
//
LOCUS       DASTKS010000574         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_92008,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000574 DASTKS010000000
VERSION     DASTKS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92008"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFQ35_11865"
     CDS             <1..447
                     /locus_tag="VFQ35_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931382.1"
                     /translation="LAKINIDAALGRLDYSARRMRGAEISANVRYAARAAEVAGPGAV
                     ILADAQTSWPIPTFGPKVLLIFHNNPLVTDEGERIVHVAHFLNPETNEADRRETIQKY
                     GVTHLLLKRSQLRRVESFLPLTESRVQLPGGYWLYTLGPELRGQGK"
     gene            556..1293
                     /locus_tag="VFQ35_11870"
     CDS             556..1293
                     /locus_tag="VFQ35_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931383.1"
                     /translation="MRKLVGLSLLAGSFVACTPLPEPHEAVLVNAPKGAPTTELAMRV
                     EEVFGLASSEIGVLSFRFTNTGSDFLHIDRVVLGFGDKKTTESLSVLSGSDLESWADA
                     IDTRNRERRYARDAAILGLAIGADILAGAAALARSKPLPPPEATGFAVAATALDRESV
                     VLAPYPEAYVLATPFGVPPNLGVSRWVALQTRDQGTCVHFVSLSAYVRERGWLQFATE
                     FRGPRSPSEWQKRRCSSPSSAPSSDAW"
     gene            complement(1267..1743)
                     /locus_tag="VFQ35_11875"
     CDS             complement(1267..1743)
                     /locus_tag="VFQ35_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931384.1"
                     /translation="MRVLVVDEDATVLNTCCAWLIGAGYEVSTQRTAGRLLSCIRHAQ
                     AEVALIEPLMNGLTSEELSLLLASCRHAGAPSVILHSRLRPLLLRTVVDPAQARGVIR
                     KTDDPEEFLRDFRALTAGVWQHRPTSVSPSPAVSGTHRIDTDGAQVIALPSVGTRR"
     gene            2026..2511
                     /locus_tag="VFQ35_11880"
     CDS             2026..2511
                     /locus_tag="VFQ35_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931385.1"
                     /translation="MQRLELADEVSQSARRFLGARPCRAMPEGTRFGLPSEAERVTQI
                     LPNAREFLEFHWSHALGAPSRVERAAEADEITQGLEWNFGVARCQLFGVELPPLGGVK
                     APLQALERAKLSRCAKPAGTSRRHLYGCLEWIRLLPVQLPCDRPELCMKTVVRNGAEW
                     A"
     gene            2508..3542
                     /locus_tag="VFQ35_11885"
     CDS             2508..3542
                     /locus_tag="VFQ35_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9931386.1"
                     /translation="MNPLRRGTIVAIAAAILFGATTPLVKRFGAHTGPFATAALLYAG
                     SALGGGVWLPRGAPSRLAARWKRIAAVALLGAALAPASLAFGLQHSGALAGALLLNCE
                     AVFTVYLARAIYREPLGTRVIVATLSMVTGGVLLALRARAGGESTTLGLVAIVVATFA
                     WALDNTLTRALADFEPREVVLAKSALGGLLSTAFAVGFGEPWAEWWAAIGLVVSGVFG
                     YGFSLRLYLRAQRELGAARTGSLFSVAPFVGAMLAFAAGDREQALLVAGAAVAFCFAI
                     YLHATEQHHHLHHHEPVEHEHAHRHDDGHHMHVHDFEVIGEHTHRHRHEAVEHDHPHG
                     ADLHHRHEHD"
BASE COUNT          606 a         1272 c         1281 g          722 t
ORIGIN      
        1 ctcgcgaaga tcaacatcga cgccgcgctc ggccggctcg actacagcgc gcgtcgcatg
       61 cgcggcgccg agatcagcgc gaacgttcgc tacgccgcgc gcgccgccga agtcgcggga
      121 ccaggcgccg tgatcttggc ggacgcgcag acgtcgtggc caataccgac cttcggcccc
      181 aaagtcttgc tgatcttcca caacaacccg ctcgtcaccg acgagggaga gcgcatcgtc
      241 cacgtcgcgc acttcttgaa cccggagacc aacgaagcgg atcgacgcga gaccatccag
      301 aagtacggtg ttacccacct tctcctcaag cgcagccagc tccgtcgggt tgaaagtttc
      361 cttcctctca ccgaaagccg tgttcagctg ccggggggct actggctgta cacgctcggt
      421 cccgagctgc gtggccaggg aaaatgagag ctcggcgtta tcgctgacac ggtgaatcgc
      481 ttcgttgccg ccgcccgcgc ggggggctcg ccgactggaa cgcaccttgc ttcggaacgc
      541 ggtcggaggt gtgttttgag aaaactcgta ggcctgtcgt tgctcgccgg aagcttcgtt
      601 gcgtgcacgc cactgccgga accgcacgag gcggtcctag tgaatgcacc caaaggtgct
      661 cctacgacgg agcttgccat gagggtcgag gaggtcttcg ggctcgcgag ctccgaaatc
      721 ggtgtgctct cgtttcgatt caccaacacg gggagcgact tcctgcacat cgaccgcgtc
      781 gtgctcggct tcggcgacaa gaagacgacc gagagcttga gcgtgctttc gggaagcgac
      841 ctcgagagct gggcggacgc gatcgacacc cgcaatcggg agcggcgcta cgcgcgggac
      901 gccgcgatac tgggactggc catcggggcg gacattttgg ccggtgcggc ggcgctggcg
      961 cgctcgaagc ccctgccccc acccgaagcg accggtttcg cggtggctgc cacggcgttg
     1021 gaccgcgaaa gcgtggtcct ggcgccctat cccgaagcgt acgtgttggc gacgccgttt
     1081 ggcgtgcctc cgaacctcgg tgtctcgcgc tgggtcgcgc tccaaactcg cgaccaaggc
     1141 acctgcgttc acttcgtttc gctcagcgcg tacgtacgtg agcgcggctg gctgcagttc
     1201 gcgacggagt tccgcggccc gcgcagccct tcggagtggc agaagcggcg ctgttcgtcg
     1261 ccgagctcag cgccgagttc cgacgcttgg taacgcgatc acctgcgcgc cgtcggtgtc
     1321 gattcggtgg gtgccgctga ccgccggtga agggctgacc gacgtcggcc ggtgttgcca
     1381 tacccccgcg gtcaatgcac ggaagtcgcg caggaactct tcggggtcgt cggtcttgcg
     1441 aatcacgccg cgggcctgcg ccggatccac cacggtgcgc aagagcagcg gtcgcaaccg
     1501 cgaatgcaag atcacgctcg gcgcgccggc gtgtcgacag ctggcgagca agagcgaaag
     1561 ctcctcgctg gtgagcccgt tcatcagcgg ctcgatcaaa gcgacctcgg cctgtgcgtg
     1621 acgtatgcaa gaaagcaagc gcccagcggt gcgctgcgtg cttacttcat agcccgcgcc
     1681 aatcaaccat gcgcagcacg tattcaatac ggttgcgtct tcatcgacga ccagaacgcg
     1741 catacctctc atcatcgtcg aatgccgctc gcctgcatcc gagctccctt cggcctgagt
     1801 caacggtcgg cacgggtggc tctttagcga aggccgagtc attcccagct tggaacgact
     1861 cgaatgagct ctatcatggc gccgcgggtg cgcaccgggc cgcgtgtctc aacttctcat
     1921 gcggttgtgc ttctttttgg cttctctttg ctcgagcgtc ggattccgca cctcgcggcc
     1981 gggccgagct ctgagggcgc gggacgcggc cgcttccaaa gttggatgca acggctcgag
     2041 cttgccgacg aggtgtccca gtcggcgcgt cgcttcctcg gcgcgagacc atgccgcgcg
     2101 atgcccgagg gtacccggtt cggtcttccg agcgaggcgg agcgcgtcac tcagatcctt
     2161 cccaatgccc gcgagttcct cgagttccac tggtcccacg cgctcggtgc gccaagccgc
     2221 gtagagcgcg cggcagaggc cgacgagatc acgcagggcc tcgagtggaa ttttggcgtc
     2281 gcgcggtgcc aactctttgg agtggaactg ccgcccctcg gtggtgtcaa ggcgcccctg
     2341 caagccctcg aacgcgcgaa gctttcgcgg tgcgcgaaac ccgccggcac atcccgccgc
     2401 catttgtacg gctgtctcga atggatacgc ttgctgccgg tgcaactgcc gtgcgaccgt
     2461 ccggagctct gcatgaagac cgtcgttcgc aacggcgccg agtgggcatg aacccgctgc
     2521 ggcgaggcac aatcgtcgcc atcgcggccg cgattctgtt cggtgccacg acgccactgg
     2581 tcaagcgctt cggtgcccat accggtcctt tcgcgactgc ggcgttgctc tacgcgggtt
     2641 ctgcgctggg cggtggcgtc tggttacccc gaggtgcccc cagtcgcttg gcggcgcggt
     2701 ggaagcggat cgccgccgtc gccctgctcg gcgcggcgct agctccagcg agcctggcct
     2761 ttggcctgca acatagcggg gcgctagcgg gagccctgct gctcaactgt gaagctgtgt
     2821 tcactgtgta tcttgcgcgc gccatctacc gggagccact cggtacccgc gtgatcgtcg
     2881 cgacgctttc gatggttacg ggcggcgtgc tgctcgcctt gcgcgctcgc gccggtggcg
     2941 agtcgaccac gttggggctc gtggcgatcg tggtcgcgac gttcgcttgg gcgttggaca
     3001 atacgctcac gcgcgcgctt gccgacttcg agccgcgcga ggtagtcctc gccaaatcgg
     3061 cgctcggggg tcttttgtcc accgcctttg cagttgggtt cggcgagcct tgggcggagt
     3121 ggtgggccgc aatcggcctc gtcgtttcgg gcgtcttcgg atacggcttc agcctgcgcc
     3181 tctacctacg cgcgcagcgc gagttggggg ccgctcgaac gggctcgctg ttctcggtcg
     3241 cccccttcgt cggcgccatg ctggcgttcg cggctggtga tcgcgagcag gcgctgttgg
     3301 tggcgggcgc tgcggtcgcc ttttgctttg ccatctatct ccacgcaacg gagcaacacc
     3361 atcacttgca ccatcacgag ccggtcgagc acgagcacgc gcatcgccac gacgacgggc
     3421 accacatgca cgtgcacgac ttcgaggtga tcggcgagca cactcaccgg catcgtcatg
     3481 aggccgtcga acacgaccac cctcacgggg cggatcttca tcaccggcac gagcacgatt
     3541 gacggtggcg tctgcgccgg ctgcgcgcgg agttcgcgca agagcgcaac tttcagcaat
     3601 cccagtcgcc gcgtgagttc gagtgtcgag cgtaagttgc agccaggctc gccgggttgt
     3661 cgaaaacgag tcactctgag cagatctggg ctacggcggc gccggggccg aatgcacgcc
     3721 gcttttgggc gacgttagcg aggaaaagtc gggaaagcgc gacgcttccg cgagctgggc
     3781 ggaggtcgct cgggtccgcg tccccggtca aatttgtcct cctgcctggt cagcgagcgc
     3841 gggtacacta tcgatagttg gacgggaaaa tttggctccg t
//
LOCUS       DASTKS010000575         5675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_983861, whole genome shotgun sequence.
ACCESSION   DASTKS010000575 DASTKS010000000
VERSION     DASTKS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983861"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFQ35_11890"
     CDS             complement(<1..246)
                     /locus_tag="VFQ35_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931387.1"
                     /translation="MNARRWLLACLAGGGLALGCDPFHTQFDDVERAVEYRASKLKPA
                     RPGLSLRVMNYNVKFGGARIDFFFDCFGDRVLMSKSEV"
     gene            complement(243..989)
                     /locus_tag="VFQ35_11895"
     CDS             complement(243..989)
                     /locus_tag="VFQ35_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931388.1"
                     /translation="MRWFTRFGIALGLSLVGCGGSSEKPPESPSSNSVALAEPKSKPK
                     PKPRAKKEPATLFPQRCTMKRGECLPPAEWVNKLCDNIYPDLALHFFAPKTPWQRLYM
                     TARADPFNASGGMSLMGDKLEPGEEVIALRRRDATKMQSTGENAGYDVLRWNGACATI
                     HDDDFTEEPPSNLRSAPIDWRRLSVEMRNALEAIPEIGEVYELRHQKCRGKSVGIISD
                     QCELYDKKLSEVVAMKVRSGAKLPQPKQVP"
     gene            1125..2075
                     /locus_tag="VFQ35_11900"
     CDS             1125..2075
                     /locus_tag="VFQ35_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET9931389.1"
                     /translation="MLRVFEVPRSGQAVERPTEGPLAPNPEYLLWVDIEAPTREELER
                     LREPFGLHPLAIEDCLTQDQRPKLEEYPSNIFVVIHELSRNGDELEGKEIHAFLGERA
                     LITVRDHHCRRVEELVLRVLGASDLYERGIAFVYYLLADAVASHNAVVLDELTEAIDD
                     IEQQVLEGENEMTLPRIFSLKHSLGSARRALSPQRDLFVSLSRLQPRWVGERTAFYFR
                     DVYDKLARTVEALESNRDLLGNVLEAHFSVVAQRTNDIVKRLTILSAIFLPLTFLTGF
                     FGQNFDHLPFHSVTFMWAAFGCCIIVPPSMLFWFWRRGWL"
     gene            2215..2859
                     /locus_tag="VFQ35_11905"
     CDS             2215..2859
                     /locus_tag="VFQ35_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931390.1"
                     /translation="MVLPSRAPDPVAPEPARDLAEIPLAEVPLTEVAQDGDELSTATL
                     RELVTEHLDFVWRSLRRFGVPAADVDDAAQQVFLIANEKSGKIRRGSERSFLVGVALR
                     VASHARRSFERRAAVEQRFSTHPTPPNPDPEELAQQREARHLLDRVLDALPEDMRAVF
                     VLFELEELSLEQIASLLNVPRGTVASRLRRAREVFHSQAKIVEAAHQGGRGAQP"
     gene            2856..3584
                     /locus_tag="VFQ35_11910"
     CDS             2856..3584
                     /locus_tag="VFQ35_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931391.1"
                     /translation="MNDPDRLMQSGANDFEARLLRAGRSDAISEQSRRVIVSGLGLGG
                     VMLWASGLMAAVQKAGKGWLTAGAVRWVGVGALSGAALWAGMKLHEPSAPVSAPHKVV
                     AATSKVAPIAPQVESAPLPPIEEAKPAEEKAKALSARARANRSSIATNDKSTLAEELA
                     MVDLARAALARKDTAQSLRLLDEYTHRFSPRRLETEATVLRIEALSAAGDRATASRLG
                     QTFLSRHPQGPYARRVRSLIGAAE"
     gene            3571..4602
                     /locus_tag="VFQ35_11915"
     CDS             3571..4602
                     /locus_tag="VFQ35_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931392.1"
                     /translation="MPPNSLRRAAVCRSSYALVRAQRLASVLVCAACTSEFPLGAWGT
                     AGHEGDSNGGTTSRGGTGAGESGEAGGGPLRGGASGSNGCQLSGTVLPANAPGADVDV
                     TTTSTDFAWPSAFDSTEIELVVETEHDRDGYFWAYQFSLAGSPASGFVGLQVNGGYQA
                     VPPNGEVAVTTMAVFWIGGAPLKAELGDVAFPDARTYLKVDNAATTPNPNTWWTIHAK
                     YPVVRCRPYRMRVARVAQEANGDIWYGATIRDVQASVDTYFGRILVPAAWGQIRSTSA
                     FMDRIGWGTPTSCSYPEYASGRFSFPTASDGNLLPLSHSNRFESPAHCGTSRFTELVG
                     GVRHELGVR"
     gene            complement(4641..>5675)
                     /locus_tag="VFQ35_11920"
     CDS             complement(4641..>5675)
                     /locus_tag="VFQ35_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HET9931393.1"
                     /translation="ATERAPSQKPVVLIVDDSPESIDILRGALGDLFQVKAAIRGRTA
                     LEIVANDSPDLVLLDVMMPDLDGYEVCRQLKANPSTASIPVIFVTTLSHVGSEARGIE
                     LGAIDYITKPYVASLVRARVKTHVALYRYQRSLEEQVRERTRELHETRLEIIRRLGRA
                     AEYRDNETGMHVLRMSHISRLLAQRTGMNELESELLLQAAPMHDVGKIGIPDRILLKP
                     GPLTPEEWETMKQHTVIGAQIIGEHPSELMQMARIVALRHHERWDGSGYPDGLSGERI
                     PRSARVVAIADAFDALLSERPYKRPWSLEDTLAFFRSQRGAQFDPSGVDALLACVDAC
                     VSVRARFSDD"
BASE COUNT          931 a         1804 c         1877 g         1063 t
ORIGIN      
        1 gacttcgctc ttcgacatca gcacgcggtc cccgaagcag tcgaagaaga agtcgatgcg
       61 cgcgcctccg aacttcacgt tgtagttcat cacgcgcaag ctgagcccgg gcctggccgg
      121 tttcagcttc gaggcgcggt actccaccgc gcgctcgacg tcgtcgaact gggtgtggaa
      181 ggggtcgcaa ccgagcgcca agcccccgcc cgccaagcag gcgagaagcc agcgccgggc
      241 gttcacggga cctgcttcgg ttgcggcagc ttcgcgccgg agcgcacctt catggccacg
      301 acttcgctga gcttcttgtc gtagagctcg cactggtcgg agatgatgcc gacgctcttg
      361 ccgcgacact tctggtggcg gagctcgtac acctccccga tctcggggat cgcctccaag
      421 gcgttgcgca tctcgacgct gagccgacgc cagtcgatgg gcgcgctgcg cagattgctg
      481 ggcggctctt cggtgaagtc gtcgtcgtgg atcgtcgcgc acgcgccgtt ccagcgcaaa
      541 acgtcgtagc ccgcgttctc tcccgtcgac tgcatcttcg tcgcgtcacg tcggcgcagc
      601 gcgatcactt cttcgcccgg ttcgagcttg tcacccatga gcgacatgcc gccggaggcg
      661 ttgaacggat ccgcgcgggc ggtcatgtac aagcgttgcc acggcgtctt cggcgcgaag
      721 aagtgcagcg ccagatccgg gtagatgttg tcgcacagct tgttcaccca ttcggccgga
      781 ggcaggcact cgccccgctt catcgtgcag cgctgaggaa agagggtcgc cggctccttc
      841 ttggctcggg gcttcggctt gggcttgctc ttgggctccg cgagcgcgac cgagttcgac
      901 gacggggact ccgggggctt ctccgaggac cctccacacc ccacgagaga gagcccgagc
      961 gcgatgccga atcgtgtgaa ccaacgcatg ccgactcgcc tcgttaacgc gctgcccccg
     1021 acggcacaag ccgaggacga ggtccggtgc ggaagtgctg gaaattttcg aggtcattgc
     1081 gcggaggcct ttgcgctttc gaagggcttg gtatcgttcg cctcgtgctg cgtgttttcg
     1141 aggtgccccg aagcggtcaa gcggtcgagc gtccgacgga ggggcccctc gctccgaatc
     1201 cggaatacct cctgtgggta gacatcgaag cgccgacccg cgaagagctc gagcgcttgc
     1261 gcgagccgtt cgggcttcac cccctcgcca tcgaagactg tctgacgcaa gaccagcggc
     1321 cgaagctcga agagtacccg tcgaacatct tcgtcgtgat tcacgagctc tcgcgcaacg
     1381 gtgacgagct cgaaggaaaa gagattcacg ccttcttggg ggagcgcgcg ctcatcacgg
     1441 tgcgtgatca tcattgccgg cgcgtcgagg aattggtgtt gcgtgtgctc ggcgcgagcg
     1501 atctgtacga gcgcggcatc gccttcgttt actacttgct ggcggacgcg gttgcgtcgc
     1561 acaacgcggt ggtgctcgac gagctgaccg aagctatcga cgacatcgag caacaggtgc
     1621 tggaaggcga gaacgagatg acgttgccgc gcatcttctc gctgaagcac tcgctcggct
     1681 cggcgcgccg tgcgctctca ccgcagcgcg acttgttcgt ttcgctctcg cgtttgcagc
     1741 cgcgttgggt cggggagcga accgcttttt actttcgcga cgtctacgac aagctcgcac
     1801 gcaccgtcga ggcgctggag agcaatcgtg atctgttggg caacgtgctc gaggcgcact
     1861 tctcggtcgt cgcccagcgc accaacgaca tcgtcaaacg tctcacgatc ttgagtgcga
     1921 tttttctgcc cctcacattt ctcaccggtt tcttcgggca gaacttcgac caccttccct
     1981 ttcactcggt gacgttcatg tgggcggcat tcggctgctg cataatcgtc ccaccgagca
     2041 tgttgttctg gttttggcgg cgcggctggt tatgatgttt gcgtctgctt ggaagttttc
     2101 gttgacacgg gaagccgctg cggaaatccg cggcatccgc cgttttcgat cggcaccgtg
     2161 ggcatcgagc gggcatcaaa gtcaggattg gagttctttt ccgaaacctg cccgatggtt
     2221 cttccctctc gagcaccgga cccggtagcg cctgagcccg cgcgtgatct cgccgagatc
     2281 ccgcttgccg aggtcccgct caccgaggtg gcccaggatg gcgatgagct ctcgaccgcg
     2341 acgttgcgag agctcgttac cgagcatttg gatttcgtgt ggaggtcgct tcgccgcttc
     2401 ggggttccgg ctgcggacgt cgacgacgcg gcccagcagg tctttctgat cgccaacgag
     2461 aagtcgggga agatacgccg agggagtgaa aggtcgtttc tagtgggggt tgcgctgcgt
     2521 gtggcgtccc acgcgcggcg ctccttcgag cggcgcgccg cggtcgagca gcggttctcg
     2581 acccacccga ctcctcccaa cccggacccc gaagagctcg cccagcaacg cgaagctcgc
     2641 catttactcg accgcgtgct cgatgcgttg ccagaggaca tgcgcgcagt gttcgtcttg
     2701 ttcgagctcg aggagctttc gctcgagcag atcgcttcgt tgttgaacgt gccgcgcggc
     2761 accgtggctt cgcgtctgcg gcgtgcgcgc gaggtgttcc actcgcaagc gaagatcgtc
     2821 gaagcggccc accaaggcgg ccgaggagcc cagccatgaa cgatcccgat cgtctgatgc
     2881 agagcggcgc gaacgacttc gaggcgcgcc tgttgcgagc cggacgcagc gatgcgatct
     2941 ccgagcagag ccggcgcgtg atcgtgtcgg ggttgggcct cggcggcgtg atgctctggg
     3001 ccagcggctt gatggcggcc gttcaaaagg ccggcaaggg atggctcacc gcgggcgctg
     3061 tgcgctgggt cggcgtcggc gcgctttctg gcgcagcgtt atgggccggc atgaagctcc
     3121 acgagccgag cgcgccggtg agcgcacccc acaaggtcgt cgccgcgacg tccaaagtcg
     3181 cgcccatcgc gccgcaggtc gagagcgcgc ctctgccgcc gatcgaagag gcgaagccag
     3241 cggaagaaaa agcgaaggca ctttcggcgc gggctcgagc gaatcgcagc tccatcgcga
     3301 cgaacgataa atcgactctc gccgaagaac tcgcgatggt agatctcgcg cgcgcggcgc
     3361 tggcgcgcaa agacacggct cagagcctgc gcttgctcga cgaatacacg caccgcttct
     3421 cgccgcgccg gctcgagacc gaggccacgg ttctgcgcat cgaggcgctg tccgccgctg
     3481 gcgatcgggc caccgcatca cgtctcggtc agacattcct cagccggcat ccgcaaggtc
     3541 cgtacgctcg cagggtgcgc tcgttgattg gtgccgccga atagtctgcg acgcgcggcg
     3601 gtttgccgct cgagttacgc gctcgttcga gcgcagcgac tcgcttcggt gttggtttgc
     3661 gccgcgtgca cgtccgagtt tcccctcggc gcgtggggaa cggcggggca cgagggcgac
     3721 tcgaatggtg gcaccacttc gcgtggcgga accggagcag gcgagagcgg ggaagccggc
     3781 gggggacctc tgcgtggcgg cgcgagcgga agcaacggct gtcagctctc ggggacggtc
     3841 ctccctgcga acgcgccggg cgcggacgtc gacgtcacga ctaccagcac tgactttgcg
     3901 tggccgagcg cgttcgactc gaccgagatc gagctcgtgg tcgagaccga acacgatcgc
     3961 gacggctact tctgggctta tcagttcagc ctcgccggta gccccgcgag cgggttcgtt
     4021 gggctccaag tgaatggagg ctatcaagcc gtgcctccca atggcgaagt cgccgtgacg
     4081 accatggccg tgttctggat cggcggcgca ccgctcaaag ccgagctcgg cgacgtggcg
     4141 ttccccgacg ctcgcactta cctcaaggtg gacaacgcag cgacgacgcc caacccaaac
     4201 acttggtgga ccatccacgc caagtatccg gtcgtgcgct gccgtcctta tcgaatgcgc
     4261 gttgcgcgtg tcgctcaaga ggcgaacggc gacatttggt acggagcgac gatacgcgac
     4321 gtacaggcaa gtgtggatac ctacttcggc cggatcctgg tgcccgccgc gtggggacag
     4381 atccggagca cgagcgcgtt catggatcga atcgggtggg gaacgcccac gtcgtgttcg
     4441 tacccggagt atgcgtcggg ccgcttttcg tttccgaccg cgtcggacgg caacttgctg
     4501 ccgctcagtc attcgaaccg cttcgagtca cccgcgcatt gcggtacctc gcgcttcacc
     4561 gagctcgtcg gcggagtgcg gcacgagctc ggggtgcgct gacgagcgaa ttgtcgagca
     4621 cgagcacgcc cgccggcgtg tcagtcgtcg ctgaagcggg ctcggacgga gacgcaggcg
     4681 tcgacgcagg cgagcagcgc atcgacgccg ctcggatcga attgcgctcc gcgctggcta
     4741 cggaagaacg cgagcgtatc ttccaagctc caaggtcgct tgtacgggcg ctccgaaagt
     4801 agcgcgtcga aggcgtcggc gatggcgacg acgcgcgccg aacgcgggat cctttcgccg
     4861 ctcagcccgt ccggataacc agagccgtcc catcgctcgt gatgtcgcag cgcgacgatg
     4921 cgcgccatct gcatcaactc ggaggggtgt tctccgatga tctgcgctcc gatgaccgtg
     4981 tgctgcttca tggtctccca ctcttcgggc gtgagggggc cgggcttcag caagatgcgg
     5041 tcgggaatgc cgatctttcc cacgtcgtgc atgggtgcag cttgaagcaa gagctcgctt
     5101 tcgagctcgt tcatcccggt cctttgcgcg agcagccgcg agatgtggct catgcgcaag
     5161 acgtgcatgc cggtctcgtt gtcgcggtac tccgcggctc tgccgagccg ccgaatgatc
     5221 tcgagccgcg tctcgtggag ctcacgagtg cgctctcgga cctgctcctc gagcgagcgt
     5281 tgataacgat acagagcgac gtgcgtcttc acccgggcgc gcaccaggga cgcgacgtac
     5341 ggcttcgtga tgtagtcgat cgcgccgagc tcgatgcctc gagcttcgct tccgacgtga
     5401 gagagcgtcg tgacgaagat tacggggatg gacgcggtgc tcgggttcgc tttcagctga
     5461 cggcaaacct cgtatccgtc gagatccggc atcatcacgt cgagcaggac cagatcgggc
     5521 gagtcgttcg cgacgatctc gagcgccgtg cgtccgcgaa tggcggcctt gacctggaag
     5581 aggtcgccga gcgccccccg caagatatcg atgctctccg gagagtcgtc cacgatgagg
     5641 accacgggct tctgactcgg tgcgcgctca gttgc
//
LOCUS       DASTKS010000576         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_539222, whole genome shotgun sequence.
ACCESSION   DASTKS010000576 DASTKS010000000
VERSION     DASTKS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539222"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1090)
                     /locus_tag="VFQ35_11925"
     CDS             complement(200..1090)
                     /locus_tag="VFQ35_11925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9931394.1"
                     /translation="MKKSEPGWELYRSFLAVVRERSLSGAARALRLTQPTLGRHIDAL
                     EEALGAPLFTRSRVGLVPTETARALVPSAEAMSSAASAMARIASGEAHEERGAVRVTA
                     SEMVGTYVLPSFFATFRRAHPSIDVELVLSNRNHDLSRREADIAVRMVKPTQGALLAK
                     KVGVIPLSLHAHPSYLAARGTPHSAAELGQHSLIGFDTAASIARLPKLDFPLSRASFA
                     FRCDNDVAQYAAIRSGFGIGACQVPLAKRDLLVPLLPSAFRLELGVWVVMHRDLKSSR
                     RTRLMFEHLVTQLKAYCAEA"
     gene            1197..2171
                     /locus_tag="VFQ35_11930"
     CDS             1197..2171
                     /locus_tag="VFQ35_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9931395.1"
                     /translation="MQKTALVVGITGGIGSETARALLSAGWKVRALHRDPERAAEALA
                     GLGPIEWVRGDALNAGDAIAAARGVSVVVHAANPPGYRNWKGLALPMLESSIAAAKAN
                     HARIVLPGTVYNFGPEAPDCVDETSPQLPVTRKGKIRVAMEQRLRDASVESVSVLIVR
                     AGDFFGPHAGNCWFSQGVVKPGRALNAVTYPGKSTAGHAWAYLPDLARTIVELLERSS
                     ELGSFEVFHFRGHSFERGIELAQATRAAAGKPQAPIRSFPWFVIYLLAPFVETFREML
                     EMRYLWTRSLFLDNRKLVAFLGAEPHTPLDEALRVTLRGLGCLPQLRV"
     gene            2452..3276
                     /locus_tag="VFQ35_11935"
     CDS             2452..3276
                     /locus_tag="VFQ35_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931396.1"
                     /translation="MVARAECKPADWTPRELPPLLERGKGAPSSSACARPGCAESTRP
                     DQAGPGGALLERACTDSPEVTAGPAPAPVVVDGVELRVLSTTPAGKSGRNWAGNRCAF
                     GLRLADGSGTAVELGPEHVPAFNTITAVVRSGSAVWLSVGFNGYTKEFPGGGNRVIAV
                     DLCAGRVVWQSKDAMSNGGLLLLDDYLISPFGFTSERRFVYVLDARSGSVLQKLPVIE
                     NVCPSKSWAPNWHPGERCDAPGQAVGAATQPRVEDGLFYVDTNTGSSAFHFKATSR"
     gene            3273..4460
                     /locus_tag="VFQ35_11940"
     CDS             3273..4460
                     /locus_tag="VFQ35_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET9931397.1"
                     /translation="MNSLVRVGSALLLLALSCSRVAATPAPKPEPHAGPSPSATPRPL
                     LRVGTTGDYAPFSKRQSEGAPVGFDVDLAAALAQDLGFELRFVEVRWPELAARVSEGA
                     FDIAMTGVTWQPARAVTGYMTRSVAHGGPCVLGDTQAHDIGVNHGGVLESWAKKRFAD
                     RNLVVVDDNLGLPALLASGRVQAIVTDSFERRSFERPGVRAQCEPALAQKVYWVAPAR
                     AAELGARVDEWLARHSEKIGVAQARWFGERQRLDAVTHIVDLLARRLAFMPFVAALKA
                     ARNLPIDDPPREAEVLEGVRRGAERLGLPEQPTLELFKLQIELSKAVQRRQSEPSTLD
                     LKEQIRPALTDLGERILQGVVDARATKALSNLGDSDLEAVSPWLLAEERQQLGNALQR
                     LSL"
     gene            complement(4482..>4783)
                     /locus_tag="VFQ35_11945"
     CDS             complement(4482..>4783)
                     /locus_tag="VFQ35_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931398.1"
                     /translation="SVEPPSSTAAGRTRILQALVLAVGLFLGAVSLAIMIGLTSSEDL
                     DAALSLFLSITAIVAIFVGRRGIQAPWLAAEPAQRALGILLWLAAIGLTLLAWGS"
BASE COUNT          833 a         1564 c         1585 g          801 t
ORIGIN      
        1 gtaggtcgcg ttgttcacgt gcccgaacgt atcgaggtga cgctcgatga tcagaacctc
       61 gtactcgtgt gcgcccaggc tcatcattcg caatcggatt tctcgctcgc gacgtgacct
      121 tcgtcaagtg ctctccaaga attcaacggc tatcgagtcc cgcaacggct cgccatgtga
      181 acttgtttgc tcggggtcgt caggcttcgg cgcaatacgc cttcagctga gtgacgaggt
      241 gctcgaacat caggcgagtg cgacggctcg atttgaggtc gcggtgcatc accacccaga
      301 ccccgagctc gaggcggaac gccgacggca acagcggaac gagcaggtcg cgctttgcga
      361 gcggaacctg acaggcccca atgccgaagc ctgagcgaat ggcggcgtat tgagcgacgt
      421 cgttatcgca gcgaaacgcg aatgacgcgc gcgagagcgg aaagtcgagc ttgggtaggc
      481 gggcgatcga ggcggccgtg tcgaagccga tgagagagtg ttggccgagc tccgctgcgc
      541 tgtgcggcgt cccccgggcc gccaagtagc tcgggtgagc gtgcagagag agcgggatga
      601 cgccgacctt cttggccaag agcgcgccct gcgtcggctt gaccattcga accgcgatgt
      661 cggcctcgcg gcgggagaga tcgtgattgc gattcgagag cacgagctcc acgtcgatgc
      721 tcgggtgagc gcggcggaac gtcgcgaaga agctcggcag cacgtaggtg ccgaccatct
      781 cgctcgcagt aacgcgcacc gcgccgcgct cttcgtgcgc ttcacccgag gcgatgcgag
      841 ccatggcgct ggcagcgctc gacatggctt cggcagacgg aaccagcgcg cgcgctgtct
      901 cggtgggaac gaggccgacc cgcgaccgcg tgaaaagcgg agcgccgagc gcttcttcca
      961 gggcgtcgat gtgccggccc agcgtcggtt gagtcaacct cagcgcgcgc gcagcgccag
     1021 acaagctgcg ttcgcgaacc acggccagga aagaccggta cagctcccac cccggctcgg
     1081 acttcttcat acaaatttgt atagcagcta tgtcgattag gccaattccg tttgatggcg
     1141 agcaggcgca tggtgtccgt gtcgccgtcg agagcgcggc gcagcggagc ccaaatatgc
     1201 aaaaaactgc gttggtcgtg ggtatcacag gaggaatcgg cagcgagacc gcgagggcct
     1261 tgctgagcgc ggggtggaag gtgcgcgcgc ttcatcgcga cccagagcgc gccgcagaag
     1321 ctctggccgg cctcggcccc atcgagtggg tccgaggaga tgcactgaac gcaggcgacg
     1381 ccatcgccgc ggcgcgaggc gtcagcgtcg tcgttcacgc agcaaacccg ccagggtatc
     1441 gcaactggaa ggggcttgcc ctgccgatgc tcgaaagcag catcgcggcc gcgaaagcca
     1501 accacgcgcg catcgtgctt ccgggcaccg tttacaattt cggtccggag gcgcccgact
     1561 gcgtcgacga gaccagtccg cagctccccg ttacgcgcaa gggcaagatc cgcgtcgcca
     1621 tggaacagcg cttgcgggac gcttcagtcg agagcgtatc ggtgctgatc gtgcgtgccg
     1681 gcgacttctt cggaccccac gcaggaaatt gctggttcag ccagggcgtg gtcaagccgg
     1741 ggcgagcgct caacgccgtc acttaccccg gaaagtccac cgccgggcac gcttgggcgt
     1801 atctgcccga cctcgcgcgc acgatcgtag agctgctcga gcgttcctcc gagctcggca
     1861 gcttcgaagt attccacttc cgggggcatt cgttcgagcg cggcatcgag cttgcgcaag
     1921 cgacacgcgc tgccgccggc aagccccagg cgccgattcg gagcttcccg tggttcgtca
     1981 tctacctgct ggcgcccttc gtcgagacat tccgagaaat gctcgaaatg cgctacttgt
     2041 ggacgcgctc tctatttctc gacaatcgca agctggtcgc gtttctgggc gccgagcccc
     2101 atacgccact cgacgaggcg ctgcgagtaa ccttgcgcgg gcttggatgc ttgccccagc
     2161 tacgagtatg agcgcgtgac tcgagagcgc cgggcggcag agctgacatg ggtccgcgcg
     2221 cggcgcggaa cccgacggct ctcgactaca ttgcgcgccg gccgataacg atcgacattg
     2281 tcgccgatgc ggtacgcgct tctgttcgtc ttgttgggtg cgtgtggttc acggccacca
     2341 acgacctcga gcgcgagccc accgatcgcg cctgaaccgg tacagccccc gactgcgccc
     2401 gtatcgagca tcgcgccgag tgcctcgccc cgagctccca gcgcccccct cgtggtcgcg
     2461 cgagccgaat gcaaacctgc ggattggacc ccacgcgagc tgccgccact gctcgaacgg
     2521 ggtaaaggtg ccccctcttc gtcggcttgt gcgcgccccg gctgcgccga aagcacacga
     2581 cccgatcaag cgggtcccgg gggcgcgctg ctcgagcgcg cctgcacgga ctcgcccgaa
     2641 gtcaccgcgg ggcccgctcc cgctccagtc gtcgtggacg gcgtcgagct ccgcgtgctt
     2701 tcgacgacac ccgcgggcaa gagcgggcgg aactgggccg gcaatcgctg cgcttttggg
     2761 ttacgccttg ccgatgggag cggcaccgcg gtcgagctcg gaccggagca tgtgccggcg
     2821 ttcaacacca tcaccgccgt ggtgcggtcg ggctcggcgg tttggctcag cgttggcttc
     2881 aacggttaca ccaaggaatt ccccggcgga ggcaatcgag tgatcgcggt cgatctctgc
     2941 gcggggcgcg tcgtctggca atccaaagac gcgatgtcga acggagggct gctcttgctc
     3001 gacgactact tgatttcgcc gttcggcttc acgagtgagc gacgcttcgt gtacgtgctc
     3061 gatgcgcgct cgggaagcgt gcttcaaaag ctgcccgtga tcgagaacgt gtgccccagc
     3121 aaaagctggg ctcccaactg gcacccgggt gagcgctgcg acgctcccgg ccaagccgtc
     3181 ggcgccgcca ctcaacctcg agtcgaggac ggattgttct acgtggacac gaacaccggc
     3241 tcgtccgcgt ttcacttcaa agcaacgtca cgatgaattc gctcgttcga gtcggttcgg
     3301 ctctccttct tctcgcattg tcgtgctcac gcgtggccgc gacgccagcg ccgaagcccg
     3361 aaccgcacgc cggcccctcc ccgagtgcga ctcctcgccc gctgcttcgg gtcgggacga
     3421 ccggagatta tgctcccttc tcgaagcgac agagcgaagg cgctccggtc ggcttcgacg
     3481 tcgacctcgc ggcggcgctt gcgcaggacc tcgggttcga gctgcgcttc gtagaagtcc
     3541 gctggcccga acttgcggcg cgggtcagcg agggggcgtt cgacatcgcg atgacgggtg
     3601 tgacgtggca gcccgcgcgc gccgtgaccg ggtacatgac tcgttcagta gcgcacgggg
     3661 ggccctgcgt gctcggtgat acccaagccc acgacatcgg cgtcaatcac ggcggcgtac
     3721 tcgagagctg ggcaaagaag cgctttgcgg atcgcaactt ggtcgtcgtc gatgacaacc
     3781 tcgggcttcc ggccctgctc gcttccgggc gcgtccaggc gatcgtcacc gatagtttcg
     3841 agcgacgctc cttcgaacgc cctggagttc gggcccagtg tgaacccgcg ctcgcgcaaa
     3901 aagtgtattg ggtcgcccct gcccgtgccg cggagctcgg cgcgagagtc gatgagtggc
     3961 tcgcacgtca cagcgagaaa atcggggtgg cccaagcgcg ctggtttggc gagcgccagc
     4021 gcctcgacgc cgtgacccac atcgtggact tgctggcacg caggctcgcc ttcatgccgt
     4081 tcgtcgccgc gctcaaggcc gcacggaacc tccccatcga tgatccgccg cgcgaggcgg
     4141 aggtgctcga gggtgttcgc cgaggcgccg aacggctcgg gttgccggag cagccgacgc
     4201 tcgagctctt caagctgcaa atcgagctca gcaaagcggt ccagaggcga caaagcgagc
     4261 cctcgacgct cgacctgaag gagcaaatcc gcccggcgct gaccgacttg ggtgaacgca
     4321 ttttgcaagg ggtggtggat gcgcgcgcca cgaaagcgct atccaatctc ggcgactcgg
     4381 acctggaggc cgtgtctcct tggctgctgg ccgaggagcg ccagcagttg ggaaacgcgc
     4441 tccagcggtt gagcctttga ctcttcgagc taccgagtgg ctcaacttcc ccaagcgaga
     4501 agcgtgaggc caatcgccgc gagccacagc aagatcccca gcgcccgctg cgcgggctcc
     4561 gcggcgagcc aaggcgcctg aatccctcgc cgaccgacga agatcgcgac gatcgccgtg
     4621 atcgagagga acaagctgag cgcggcgtcg agatcttcgc tgctggtgag cccaatcatg
     4681 atggccaggg acaccgcccc gagaaagagg ccgaccgcga gcacgagcgc ctgcagaatt
     4741 cgagtgcgtc cggccgcggt cgaggacggc ggctcgacgc tga
//
LOCUS       DASTKS010000577         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1878744, whole genome shotgun sequence.
ACCESSION   DASTKS010000577 DASTKS010000000
VERSION     DASTKS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878744"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1215)
                     /gene="mutY"
                     /locus_tag="VFQ35_11950"
     CDS             complement(118..1215)
                     /gene="mutY"
                     /locus_tag="VFQ35_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9931399.1"
                     /translation="MNTVAPTRSTPPLSPALLEWYDQVRRDLPFRRTRDPYAIWVSEV
                     MLQQTQVATVLSYFTRWMQRFPNVGALASASEDEVLHAWQGLGYYSRARSLRRAAQAV
                     VERFDGSLPGTVEELRTLPGIGPYSAGAIASIAFGERTPVVDGNVVRVICRLFALRGD
                     PTKAAQRKLIWQRAAELVPSERPGDFNQALMELGATVCTPQSPRCALCPLRRHCRALA
                     LGIQEDLPELPARAAITEVHMAAAVVHFRGRVLVVKLAADAPRWASMWQFPNVERRAR
                     ESAEKAAVRAVRELVSLEVAAARTVSSVKHSVTRYRITLDAVLCGSKSNRLGALSHVE
                     AAGWKRPEELDELAMPAAHRRIARSLAEVLR"
     gene            1292..>2218
                     /locus_tag="VFQ35_11955"
     CDS             1292..>2218
                     /locus_tag="VFQ35_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931400.1"
                     /translation="MPKPMPDPLIVPLEAIRTDGWFERMGEGIGSFQALCDIIGARFF
                     AFAMITGARITSLTVDRRSPDNTIVEFVVGGDSNEIDAADTQRLSLAEFRLRLVNALV
                     TDEPIGLPPRRETDVEAVQLHIGVRYLLLAPLYGYALRELTVDYDGSRIRLFHDGVEE
                     LYPLGAFRARLRTHVREELERVQRPQSRSAIDLSRLTEAEQAASRGDHVRVLELLGAW
                     PAPLAIFLRTPEGQTLSMDTRALISRALGLLGSACVTLGELGKGEEVLRLAVQYAGDS
                     NGASDVYLRLGEAMMSDGRSGEAIGPLRRAANL"
BASE COUNT          354 a          773 c          728 g          363 t
ORIGIN      
        1 gcccgagctt gccgcccgga aactcccaga gcccaccgag gtgcacggcc tcggggcgtc
       61 gtgtaatgag cagctccgtg ccgcgccata cgagcgcgat cgcaacttcc accactttca
      121 tcgcagtacc tcggcgagtg accgagcgat gcgccggtgg gcggccggca tcgcgagctc
      181 gtcgagctcc tcggggcgct tccatcccgc cgcttcgacg tgcgaaagcg cgccgagccg
      241 gttcgatttc gagccgcaca gcactgcgtc cagggtgatg cggtagcggg tcacgctgtg
      301 cttcacgctg ctgacggtgc gcgccgcggc cacttcgagc gacacgagct cgcgcaccgc
      361 gcgcacagcc gctttttcgg cgctctcgcg cgctcggcgt tcgacgttcg gaaactgcca
      421 catcgacgcc cagcgcggcg catcggccgc gagcttcacg acgagcactc ggccgcgaaa
      481 atggacgacc gccgccgcca tgtgcacttc ggtgatcgcc gcacgcgcgg gcagctccgg
      541 caggtcctct tgaatgccga gcgcgagggc acggcagtgt cgcctcagcg ggcatagcgc
      601 gcagcgtggg ctttgagggg tgcacacggt cgcgccaagc tccatcagcg cctgattgaa
      661 gtcgcccggg cgctcgctcg ggacgagctc cgccgcgcgc tgccagatga gcttgcgctg
      721 cgcggccttg gtcgggtcgc cccgcagagc gaacagccgg cagatcacgc gcaccacgtt
      781 tccgtcgacg accggcgttc tctctccgaa agcaatgctt gcgatggccc ccgccgagta
      841 gggtccgatg cccggcaatg tgcggagctc ttcgaccgtg cccgggagcg acccgtcgaa
      901 gcgctcgact acggcctgtg cggcccgccg taagctccgc gcgcgggagt agtagcccaa
      961 gccctgccaa gcgtgcagca cctcgtcctc gctggcggaa gccaacgccc caacgttcgg
     1021 gaagcgctgc atccagcgcg tgaaatacga cagcacggtc gcgacctggg tttgctggag
     1081 catcacctca ctcacccaaa tcgcataagg gtcgcgggtg cgccgaaatg gaaggtcacg
     1141 gcgcacttgg tcgtaccatt cgagcagggc aggcgacagg gggggcgtcg agcgtgtcgg
     1201 agcgacggtg ttcacttcgt gcgtcgctcc ttctagcgat gctcgccccc gaccgaaagc
     1261 ggcttgtggt gcggcggaaa agcgtgtgaa aatgccgaaa ccaatgcccg accccctcat
     1321 cgtcccgctg gaagcgattc gcaccgacgg ttggttcgaa cgcatgggcg aggggatcgg
     1381 cagcttccaa gcgctgtgtg acatcatcgg agcgcgtttt ttcgccttcg cgatgatcac
     1441 cggcgcgcgc atcacttcgc tcaccgtcga tcgccgcagc cccgacaaca ccatcgtgga
     1501 gttcgtcgtc ggcggcgatt cgaacgaaat cgacgcggcc gacacgcaac gcctgtcgct
     1561 cgccgagttc cgcctgcgcc tcgtgaatgc gctcgtcacc gacgagccga ttgggctccc
     1621 gccgcgccgc gaaaccgacg tcgaggcagt gcaattgcac atcggcgtcc gctatttgct
     1681 gctcgcgccg ctctatggct acgccctccg ggagctcacg gtcgactacg acggctcgcg
     1741 catccggctc tttcatgacg gcgtcgaaga gctctatccg ctcggagcat tccgcgcgcg
     1801 tctgcggacc cacgtgcgcg aggagctcga acgcgtccag cgcccccagt cacgcagcgc
     1861 catcgatctc agccgtctga ccgaggccga gcaggccgca tcgcgcggcg accacgtccg
     1921 cgtgctcgag ctgctcggag cttggccggc gccgctcgcc atcttccttc gcacgccaga
     1981 ggggcaaacg ctgtcgatgg acacgcgggc gctcatctct cgagcgctcg ggcttctcgg
     2041 gtcggcgtgc gtgacgctcg gcgaactcgg gaagggcgag gaagtgcttc ggctggccgt
     2101 ccagtacgcc ggtgacagca atggcgcttc ggatgtctac ctgcgtctcg gcgaagcgat
     2161 gatgagcgac ggtcgctcgg gcgaagccat cggtccgttg cgtcgcgcgg cgaacctc
//
LOCUS       DASTKS010000578         5234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_93413,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000578 DASTKS010000000
VERSION     DASTKS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93413"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFQ35_11960"
     CDS             complement(<1..391)
                     /locus_tag="VFQ35_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET9931401.1"
                     /translation="MPRLETISQRVYRDAPHASPHQGRRSRTSGRFAEAATRLVFLAL
                     VAACGVFFLPVSAARAAAFDVNDTSWEGTSELLALARERLGRERIRIVKKLDYGALKP
                     ADGVLILHPEVELEFREVSAFLAAGGRL"
     gene            554..739
                     /locus_tag="VFQ35_11965"
     CDS             554..739
                     /locus_tag="VFQ35_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931402.1"
                     /translation="MLPPFIIDQIRRREEEESRRREHERPRLELPVDVYQPAAPKPRG
                     DEPEPDGERGVIILDLG"
     gene            819..2579
                     /locus_tag="VFQ35_11970"
     CDS             819..2579
                     /locus_tag="VFQ35_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931403.1"
                     /translation="MRRPALSLRHSSLLSPLLVASAIAAAGCYSAGEGQTPPEDRIYF
                     PVGLALANDSKHLLVANSDFDLQYNAGTLNVLDVGALHPLLTVPCGNNDDCAAASARC
                     RAESPEGSDCDSRPVCDVTLGLCKQTPADDSASPVVCGSSLRQRELKEQLLSPGACDY
                     IDPARLPNGGTLLRSEVQIGSFATDVVYRERPPSSDPSTFAGRAFLPVRGDATLHWID
                     VSPDGSLECGQGSGKVCDDLHRAGNHEEENTRNLRLAPEPFALDASPDGEAVLVTNQT
                     TGTVSLFVNDWNSPNGAALQFAVTGLPLRPMGIAALPVPAYASALGSYYPPGFLVTFR
                     NAAEVDLLRYYDDQNTALMQGGTPVARPYAARTAVSGIRINSVGTDSRGIAVDGVARA
                     EAELACFSAIGLDPSCIASNTCLEAIPEAQRQAFRNCLNTASATPLDVFVANHAPATL
                     LLGRTTPVQNALESTELPNFYDSLPLTLGPSRVVIGKVIVGVDEQGKNVLERRVFVVC
                     FDSRRIFVYDPHARRMDAEIVTGRGPHALAVDEKHGWLFVGHFTDSFIGVVSLDRRFP
                     KTYGTTLAIIGEPTPPRASK"
     gene            2624..>5234
                     /locus_tag="VFQ35_11975"
     CDS             2624..>5234
                     /locus_tag="VFQ35_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931404.1"
                     /translation="MSGALAAVVALGSSCSRQTITVLLHPFQASGDVTYACRTPQGEP
                     RPVADCNPAAIDRGELDLFALMTQTATGEVAVINVPNNPASLHSGEGIVDVDPSVPGY
                     GFLHVGGLPSKIVTTPGGSLSVVAVTEPGKPGLFGLPTRCVGAPIPGASGAAGTDRDL
                     ISWPACSLLVPSSALAGSGPPELPVAPGSIAIVNAPESDTLCDGRPSPRAAMVEQNAD
                     NSVDLSCVAEVALPTGERGPKGRRKIVVTLPDRGSIAIIDAAWLAQGTTPGDFSPCLI
                     EKELPLSPTFTPMSQVLPSDLQVNEMSSASQAPQSGAFKARPSGIALDGTTLYVADNE
                     TPVIHVVDLSNPCNPVEQAPLLTRSLDRPDRVVTTSKVAVSPLTPSGRKFVYAIDQDD
                     SPTASIMAFDVSPGATDRTPIVRPRSPLIPGEPPDRILFPGAPRDIKFFERDRPIVDP
                     ATGNVVEGLACDPTPGNTGIGTRYRSAADYASGARAVELRGVFGAALLTSGQVAVIDV
                     EDFDAPCRRPVTTNSAATEDFRGCQGDPKLEKTSSLFIEANGTRTVTDEVSCRMVEPH
                     RARAGSLGLTDATRGIHAPTLRGFPQLRIPDQAVQSGPEDRPKLLAVDFALRTKDEPS
                     AAPAQVFVGTGLYSSASRENPLDVAPNSAQRNSIALPFNEPRAYNPNESLSLVYEGFV
                     TDALPAGFVRVDDPSMPFTGSAFTLHDPSVAFCDRGVHDEELMSRIGERLGVTGDAAL
                     QSFAQSHSDSVVLVGEFPPADDPSWINSQCGRDACIKEFGDFDPTIPSATPSIEREFR
                     IVDALQQWLKLEPKPNPSLSEKGQEAELAVRNGMAQCCFPSGTPYVVRASNHWVLRSD
                     AYGTRHKVIAARQM"
BASE COUNT          912 a         1795 c         1640 g          887 t
ORIGIN      
        1 cgagtcgccc tccggccgcc aaaaacgcgc tgacttcgcg gaactcgagc tcgacctccg
       61 gatgcagaat caaaaccccg tccgcaggct tgagcgctcc gtaatcgagc ttttttacaa
      121 tccggatgcg ctctcgaccg agtcgctcgc gcgccaaagc caggagctcg ctcgttccct
      181 cccagctcgt gtcgttcacg tcgaaggcag ccgcacgcgc ggcgctcacc ggcaaaaaaa
      241 acactccaca ggcggccacg agcgccaaaa agacgaggcg agtcgcggct tcagcgaagc
      301 ggccggaggt tcggctgcgt cgtccctggt gtggggatgc gtggggcgcg tcgcgataga
      361 cgcgctgcga gatcgtctcg agacgaggca tggctaactt actcgccgaa ggggccgtat
      421 aaaagagcat gacccgcgga actgcgaagg tggcagattt ggtcgccgtc cttgacccgc
      481 gtgcatgcgt cgctaaatta gctctagatc caatggcctg cccaagaggt gcgtcggaaa
      541 ggcgaggcta atcatgcttc ctccgttcat catcgaccag attcgccgtc gggaggaaga
      601 ggagagccgt cgccgagagc acgaacgacc gaggctcgag ctgccggtcg acgtgtatca
      661 accagccgcg cccaagccgc gcggtgacga gcccgagccg gatggcgagc gcggcgtgat
      721 catcctggac ctcggctgag gcaacgggcc gagcgccttc ggtcgatacg cggtcggcaa
      781 gggtcgacat tcccacacgg tgatataggc tgcgcgccgt gcgacggccc gccttatcgc
      841 tcagacacag ctccctcctt tcgcctcttc tcgttgcgtc cgcaattgcg gctgctggct
      901 gctactcggc aggtgagggg cagaccccac ccgaggaccg aatctacttc ccggttgggc
      961 tcgcgctcgc caacgactcg aagcacttgc tcgttgcgaa cagtgacttc gacctccaat
     1021 acaacgccgg cacgctcaac gtcctcgacg tcggagcctt gcacccgttg ctcaccgtgc
     1081 cgtgcggcaa caacgacgac tgtgcggcag cgagcgcacg ctgccgcgcc gagtctcccg
     1141 aaggctccga ctgcgacagc cgtccggtgt gtgacgtcac gctcgggctc tgcaagcaga
     1201 cgccggccga cgacagcgcc tcgcccgtag tctgcgggtc ttcgcttcgt caacgggagc
     1261 tgaaggagca gctgctcagt ccgggcgcat gcgactacat cgatccggcg cgcctaccga
     1321 acggcggcac tctcctgcgc agcgaggtgc agatcggctc cttcgcgacc gacgtcgtat
     1381 accgcgagcg ccctccctcg agcgacccat cgacgtttgc gggccgggcg tttctccctg
     1441 tacgcggtga cgcaaccttg cactggatcg acgtgagccc cgacggctcc ctcgaatgcg
     1501 ggcaggggag cggcaaggtt tgcgacgact tgcatcgcgc cggcaatcac gaagaagaaa
     1561 atacgcgcaa cttgcgcctc gctccggagc cgttcgcgct cgatgcaagc ccggacggtg
     1621 aggcggtgct cgtcacgaac caaactacgg gcacggtttc gctgttcgtg aacgactgga
     1681 actcgccgaa cggcgccgcg ctccagttcg cggtgaccgg cttgcccttg cgcccgatgg
     1741 gcatcgcagc gctgcccgtt cctgcgtacg cctccgcgct cggcagctat taccctccgg
     1801 ggttcctcgt caccttccgc aacgcggccg aagtcgactt gcttcggtac tacgacgatc
     1861 agaacaccgc gctcatgcag ggcggaacgc ccgtcgcacg cccctacgcc gcgcgtacgg
     1921 ccgtgagcgg catccgcatc aactcggtcg gcaccgattc gcgcggaata gccgtcgacg
     1981 gcgtcgcgcg agccgaagcc gagctcgcct gcttcagcgc gatcggcttg gacccgagct
     2041 gcattgcttc caacacgtgc ttggaagcaa ttccggaggc tcagcgccaa gctttcagga
     2101 actgcctgaa tacggcgtcc gcgaccccgc tcgacgtgtt cgtcgcgaat cacgcgcccg
     2161 ccacgctctt gctgggccga acgacgccgg ttcagaacgc gctcgagagc acggagctcc
     2221 cgaacttcta cgatagcttg ccgctcaccc tcggcccctc tcgcgtggtg atcggtaagg
     2281 tcatcgtcgg cgtcgacgag cagggcaaaa atgtgctcga acggcgcgtc ttcgtcgtgt
     2341 gcttcgattc gcgccggatt ttcgtgtacg acccccacgc gcgccggatg gacgccgaaa
     2401 tcgttaccgg tcgcgggccg cacgcgctcg cggtcgacga aaagcatggt tggctgttcg
     2461 tcggccactt cacggactcg ttcatcggcg tggtgagctt ggaccgccgc ttcccgaaga
     2521 cgtacggaac taccttggcc atcatcggcg agccgacccc gcccagagct tccaagtgaa
     2581 aatgcccgaa aagtctcccc gtgttgctcg tcgtgtcttg accatgagcg gcgcgctcgc
     2641 cgctgtagtg gcgctcggca gcagctgctc acgtcagacg atcaccgtcc ttctgcaccc
     2701 gtttcaggcg agcggcgacg taacgtacgc gtgccggact ccgcagggtg agccgcgccc
     2761 ggtcgccgac tgcaaccccg ccgcgatcga tcggggcgaa ctcgacctgt tcgcgctgat
     2821 gacacaaacc gcgacgggcg aggtggcggt catcaacgta ccgaacaatc cggcatcttt
     2881 gcactcgggc gaagggatcg tggacgtcga tccgagcgta cccggttacg gctttctgca
     2941 cgtgggcggg cttccgtcga agatcgttac gacgcctggt ggctctctca gcgtagtcgc
     3001 ggtcaccgag cctggaaagc ccggtctctt cgggctgccg acccggtgcg tcggcgctcc
     3061 catccccggc gcttcgggag cagccggaac cgatcgggac ctcatcagct ggcccgcgtg
     3121 ttcgttgctg gtcccgtcct ctgccctcgc cggcagcggt ccgccggagt tgcccgtcgc
     3181 ccccgggagc atcgccatcg tcaacgcgcc cgagagcgat accctgtgtg acggtcgccc
     3241 gagtccgcgc gccgcgatgg tggagcagaa cgccgacaac tcggtcgatc tctcctgcgt
     3301 ggcagaggtc gcgttgccaa ccggtgagcg aggccccaag ggcaggcgca agatcgtcgt
     3361 cacgctgccg gatcgcggtt cgatcgcgat catcgacgcc gcttggctgg ctcaaggcac
     3421 gacgccgggc gacttctctc cttgcctgat cgaaaaggag ctcccgctct cgccgacgtt
     3481 cacgcccatg tcgcaggtgc tgccgagcga cttgcaggtg aacgaaatga gcagcgcttc
     3541 gcaggcgccc caatcgggcg cgttcaaggc tcgtccctcg gggatcgcgc tcgacggcac
     3601 cacgctttac gtggctgaca acgaaacgcc ggtcatccac gtggtggatc tctcgaaccc
     3661 gtgcaacccg gtggagcaag cgccgctgct cacgcgctcc ctcgatcgtc cagatcgagt
     3721 cgtgacgacg tccaaagtcg cggtcagccc gctgacgccg tctgggcgta agttcgtcta
     3781 cgctatcgac caggacgaca gcccgaccgc aagcatcatg gcgttcgacg tgagcccggg
     3841 cgcgacggac cgcacaccca tcgttcgtcc gcgttcgccg ctcatccctg gcgagccacc
     3901 cgaccgcatc ttgtttccgg gcgcgccgcg cgacatcaag ttcttcgagc gcgatcgacc
     3961 catcgtcgac cccgccaccg gcaacgtcgt ggaagggctc gcgtgcgatc cgacgccggg
     4021 caacaccggt atcggaactc gctatcgctc tgcggcggac tacgcgagcg gagcgcgcgc
     4081 ggtcgagctt cggggcgtgt tcggcgccgc gctgctcacg agcgggcaag tcgcggtcat
     4141 cgatgtggaa gacttcgacg cgccctgtcg acgacccgtc acgaccaaca gcgccgcaac
     4201 ggaggacttt cgcggctgtc agggggatcc gaagctcgag aagaccagca gccttttcat
     4261 agaggcgaat ggcactcgca cggtcactga cgaagtcagc tgccgaatgg tggagccgca
     4321 tcgcgcgcgc gcgggttctc tcgggctcac cgatgcgacg cgcggcattc acgctccaac
     4381 gctgcgcggc ttcccgcagc tgcgcattcc cgatcaggcg gttcagtccg gccccgagga
     4441 ccggcccaag ctcctcgcgg tcgacttcgc gttgcgaacc aaggacgagc cgagcgccgc
     4501 gcccgcgcag gtcttcgtgg gtacgggcct ttacagcagc gcgtccaggg aaaacccgct
     4561 cgacgtggct cccaatagcg cgcagagaaa ttcgatcgcg ctgccgttca acgagccacg
     4621 cgcttacaat ccgaacgaga gcctatccct cgtgtacgag ggcttcgtca ccgacgccct
     4681 ccctgcgggc ttcgtgcgcg tcgacgatcc gagcatgccg ttcacgggga gcgccttcac
     4741 gttgcacgac ccgagcgtcg cgttctgcga ccgcggcgtc cacgatgaag agctcatgag
     4801 ccgcatcggc gagcgtctcg gcgtgaccgg agacgcggcg cttcaaagtt ttgcgcaaag
     4861 ccatagcgac tcggtggtgt tggtggggga gttcccaccc gccgacgacc cctcgtggat
     4921 caactcgcaa tgcgggcgcg acgcctgcat caaagagttc ggcgacttcg atccgacgat
     4981 cccgagcgcc acaccgagca tcgaacgcga atttcgcatc gtcgacgcgc tccagcagtg
     5041 gttgaagctc gagccgaagc caaacccgag cttgagcgaa aagggccagg aagcggagct
     5101 cgcagtgcgc aacggcatgg ctcagtgctg cttcccgtcg ggaacgccgt acgtcgtccg
     5161 cgcttccaat cattgggttt tgcgaagcga cgcctacgga actcgtcaca aggtgatcgc
     5221 cgcgcgtcag atgc
//
LOCUS       DASTKS010000579         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1432999, whole genome shotgun sequence.
ACCESSION   DASTKS010000579 DASTKS010000000
VERSION     DASTKS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432999"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1059)
                     /locus_tag="VFQ35_11980"
     CDS             complement(1..1059)
                     /locus_tag="VFQ35_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931405.1"
                     /translation="MTARRLLIALLLASAPAGAQTAPPPQPSYGAPPGYGAPAPQPPY
                     GAQPYPAQPYTGQQPAYAYPAYAPSRRSRSGAEIAALYGVSVAYGVGVGVWFDAELGV
                     KDPAGALIAPAVLGIAAPIGVYVADSPMRRGLPAAIAAGAVVGAGEGIGIASLQFVRS
                     TKSDAWGFRGLSRATTIGATLGVIGGGAFGYLQEPSPRSSVLVTSSVAWGSTIGAMFG
                     YGASAKDAGYGRSNDQAALGGFIGYNVALVGAGALSMVYIPTTTSLEWMWAGAGIGFA
                     ASLPVYLLYAGSDSPAKRGLLFSATATTLGLGAGALFTARTTESAEASRPTFARLTGV
                     APLAQPGAVGLAAFGELF"
     gene            complement(1062..2060)
                     /locus_tag="VFQ35_11985"
     CDS             complement(1062..2060)
                     /locus_tag="VFQ35_11985"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9931406.1"
                     /translation="MAHVAVLGAGAWGTALSRQLAERGERVLLWSHQAEHVAALRAQR
                     ENVEFFPGYRLHEAVEPEHDLGRVLAGARAVVVVVPTTAVRATLERAREFLQPGAIVV
                     LASKGIETTSLMLLTEVVGDVLGGESEASAVALSGPSFAEEVARGLPTNLVAASRSAE
                     SSRAVQQLLSSERLRVYTSSDPVGVQVGGALKNVMAIAAGASDGLGFGHNTRAGLITR
                     GLAEMARLATAKGGEASTLSGLSGLGDLVLTATGALSRNRTLGFELAQGRSLSEVLSS
                     LGHVAEGVTTAKSAHDLGARLHVDLPITGEVYRVLYEGKPVYDAVQGLLHRLPGKE"
     gene            2129..3211
                     /locus_tag="VFQ35_11990"
     CDS             2129..3211
                     /locus_tag="VFQ35_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931407.1"
                     /translation="MQCIRIPVGWALVAVVASASVGCASRESMGQRSLTILGAGVVND
                     PKNRSLRFDLLKFGLEQFCAEMSARGLALKMSDDEPVMGRFYASACNTQTLDEETRKS
                     FVVQYAGSGFASSPQGGRVGFTTTGLVEYAPDFLMKDGALYVYFRPRVVDATGFQTLL
                     VESQLTASAIKIFGVNPDAFGRKVVDAELRRGFTVVRYGSSGETDFGLGVIALGEKPY
                     HPFHVDTEDKQVLVNERTEVHLGQQDYVGPLNVSEDGQALYVTLKLEGAQAIDALLVP
                     EAVGKSAIDAFTKQPGPVAISAPVLLNEPVTRGALWKRYVSVPKGRYYLVLDNSAAAG
                     NVAPPAGPADGQSARTDVLVLRGDQP"
     gene            3354..>3567
                     /locus_tag="VFQ35_11995"
     CDS             3354..>3567
                     /locus_tag="VFQ35_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931408.1"
                     /translation="MNAKNLRFLFWPLWFVLVPALLGYLTVTLLAPVDDFTPTSALEQ
                     ILWRIRDQRVPAMIVFFAAYEMLLYRY"
BASE COUNT          603 a         1200 c         1189 g          575 t
ORIGIN      
        1 ctagaagagc tctccgaacg cggcgagccc gaccgccccg ggttgagcca gtggcgcgac
       61 gccggtcaat cgagcaaagg tcggccggct cgcctccgca ctctcggtgg tgcgggcggt
      121 aaacagggcc cccgctccca accccaaggt cgtagccgtc gcgctgaaca gaagcccgcg
      181 tttggctggc gagtcactgc cggcgtagag gaggtaaacg ggaagcgacg ccgcgaatcc
      241 gatgccggcg cccgcccaca tccactcgag ggaggtcgtg gtcgggatgt agaccatcga
      301 gagcgctccc gcgcccacca gggcaacgtt gtatccgatg aacccaccga gcgcggcttg
      361 gtcattcgag cggccgtagc ccgcgtcttt ggcggaggct ccgtatccga acattgcgcc
      421 gattgtgctg ccccacgcga cggagctagt cacgagcacg ctcgatcgcg gcgagggctc
      481 ctgtaagtag ccgaaggctc cgcctccgat gacgcccagc gtggcgccaa tcgtcgtggc
      541 gcgcgacagc ccgcgaaacc cccatgcatc gctcttcgtc gaccgcacga attggagcga
      601 cgcgataccg atcccctcgc ccgctccaac gaccgcaccc gccgcgattg ccgcgggcaa
      661 gccgcgtcgc atcggagagt cggcgacgta gacaccgatg ggagcggcga ttccgagcac
      721 ggccggcgca atgagcgcgc cggcgggatc tttgacgcca agttcagcgt cgaaccagac
      781 gccgacgccg acgccataag caacggaaac cccgtagagc gcggcaattt cggcgcccga
      841 ccgcgagcgt cggctcggcg cgtacgccgg gtacgcatac gctggttgct ggcccgtata
      901 aggctgtgcg ggatatggct gcgccccata cggcggttgc ggcgcgggcg ctccgtaacc
      961 gggaggcgcg ccgtacgacg gttgcggcgg cggggctgtc tgcgcgcctg caggggcgct
     1021 cgcgaggagc aaagcgatta agagtcggcg agcagtcacg ttcattcctt gccagggagt
     1081 cggtgcagaa ggccctggac cgcatcgtac acgggcttac cttcgtacag cactcgatag
     1141 acctcgccgg tgattggaag gtcgacgtgg aggcgcgcgc cgaggtcgtg cgcgctcttc
     1201 gcggtggtaa cgccttccgc aacgtggccc aagctcgaca acacttcgga aaggctgcgc
     1261 ccttgcgcga gctcgaagcc aagcgtgcgg ttgcgcgaga gtgcacccgt cgcggtcagc
     1321 acgagatctc cgaggccgga gagaccggag agagtgctcg cttcgccgcc cttggcggtc
     1381 gcgagccgcg ccatttccgc gagcccgcgg gtgatgagcc ccgcccgcgt gttgtgcccg
     1441 aagccgagcc cgtcgctcgc gccggcggcg atcgccatca cgttcttgag cgctccgccg
     1501 acttgcacac cgactgggtc ggagctcgtg tagacgcgca agcgctcgct cgacaaaagc
     1561 tgctgcacgg cgcgtgacga ctcggcgctc cggctcgctg cgaccaggtt cgtcgggagc
     1621 cctcgcgcga cttcttcggc gaagctcgga cccgaaagcg cgaccgcgct ggcctccgac
     1681 tcgccaccga ggacgtcgcc aacgacctcg gtgagcagca ttagcgaggt cgtctcgatg
     1741 cctttgctgg cgagcaccac gatggctccg ggctgcaaga actctcgcgc ccgctcgagc
     1801 gttgcgcgca cggccgtggt cggcacgacg acgacgaccg cgcgcgcgcc agcgagaacg
     1861 cgcccgaggt cgtgctcggg ctcgactgct tcgtgcaacc ggtaaccagg gaaaaactcg
     1921 acattttccc gctgggctcg aagcgcagcg acatgctccg cttgatgcga ccagaggagc
     1981 acgcgctcac cgcgctccgc gagctggcgc gacagggcgg taccccaggc gcccgcaccg
     2041 aggactgcga cgtgggccat gggtcggcac tcttcgtgct ttccgccgga gtgtcaactc
     2101 cccccgagga tcggctatgg tgctctcgat gcagtgcatc cgcattccgg tcggctgggc
     2161 actcgtcgcg gtcgtcgcca gcgcttcggt gggctgcgcg tcgcgcgagt cgatgggaca
     2221 gcgctcactc acgatcctcg gcgcgggcgt cgtgaacgat cccaagaacc gaagcttgcg
     2281 cttcgacctc ttgaagttcg ggctcgagca gttttgcgca gaaatgagcg cgcgcgggct
     2341 ggctctgaag atgagcgacg acgagccggt gatggggcgc ttttacgcga gcgcctgcaa
     2401 cactcaaacc ctcgacgaag agacgcgcaa gtcgttcgtc gtacagtacg cggggagcgg
     2461 gttcgcgagc agcccacagg gtggacgcgt cggtttcacg acgactggcc tcgtggagta
     2521 cgcgcccgac ttcctgatga aggacggcgc gctgtacgtg tacttcaggc cgcgagtcgt
     2581 cgacgcgacc gggtttcaaa ctttgttggt cgagtcgcag ctgaccgcga gcgcgatcaa
     2641 aatcttcgga gtaaacccgg acgccttcgg acgtaaggtg gtcgacgcgg agctccgccg
     2701 cggcttcacc gtcgtacgtt acggcagctc gggcgagacc gacttcgggc tcggcgtcat
     2761 cgcgctcggt gaaaagccct atcatccctt tcacgtcgac accgaagaca agcaggtgct
     2821 cgtcaacgag aggaccgagg ttcatctcgg ccaacaggac tacgtgggtc cgctcaacgt
     2881 gagcgaggac gggcaggccc tgtacgtgac gctcaaactc gagggggctc aggcgattga
     2941 tgcgctcctg gtccccgaag cggtcggaaa gagcgcgatc gacgcgttta ccaagcagcc
     3001 gggtcccgtc gccatcagcg cccccgtgct cttgaacgaa cccgtcacgc gcggcgcgct
     3061 gtggaagcgc tacgtcagcg tgcccaaagg ccgatattat ttggtgctcg acaatagcgc
     3121 cgcagccggg aacgtcgcgc ccccggccgg ccccgccgac ggccagtccg cacgaaccga
     3181 cgtgctcgtg cttcgcggcg accagccgta gccgccctcc ccgagcggct cggcgagtgc
     3241 aatttcgttt tcggccgaaa ccccggggcg gcccgcgatt cgccgccagg tgatgcccca
     3301 ttgctcgagc ggggggtccc ggctagaacc agcgggctcg ccgagccgaa atcgtgaacg
     3361 ccaagaatct tcgcttcctg ttctggcccc tgtggttcgt gctcgtgccg gctctgctcg
     3421 gctacttgac cgtgacactg ctggcgccag tcgacgactt cacgccgacc tcggcgctcg
     3481 agcagatctt gtggcgcatc cgcgatcagc gcgtgcccgc gatgatcgtg ttcttcgctg
     3541 cgtacgagat gctgctgtat cgatatc
//
LOCUS       DASTKS010000580         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1208604, whole genome shotgun sequence.
ACCESSION   DASTKS010000580 DASTKS010000000
VERSION     DASTKS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208604"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFQ35_12000"
     CDS             <1..1107
                     /locus_tag="VFQ35_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931409.1"
                     /translation="AGELRFRATPKLDLSAIGEPLTHNLHAELALTELRANSVSADSD
                     CPSLRSPSLTVRLASSERDAELLAKIADLRFGWGDFKARTDTELEVLLRRPDEEDAAP
                     PTLSFSLKNRDVSLASGTGKKSGWDASLPNLGVTGKLALGDKNAGWIEIALDRLQGRI
                     GGSRVASRLQAHVKVAELDIARKHSRFSADVELGPADVQTESDHVRDWWARIALGSAL
                     VSVQRNLDLSAIFRANLRDATPGLAVLADSGSVPDLIARNLKLEQLEVTGILQRRCRL
                     TDFIITQASGGPLSARGRLDSTSEVTRAALLLRVNGLEAISAGVALGPDGTGVTPLAG
                     DDWLREEREKMERAAREVLEGPCASEHGACGDDS"
     gene            complement(1223..1789)
                     /locus_tag="VFQ35_12005"
     CDS             complement(1223..1789)
                     /locus_tag="VFQ35_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET9931410.1"
                     /translation="MSRENWFFALPVDGAFVLELPPLPPNFRRFQPEDVHVTLCFLGP
                     CGEAAAHLALAELDARSQEFVRPAIEVRLGTVVPMGARRAYSALSALLSEGREATESL
                     IASLRDPLAAAAGAPREQRAPKAHVTVARPSRRATPAERRAGLAWAGACELQSVTARL
                     DRVCLYRWSEDRAARMFRIAAERPFRAP"
     gene            2186..>2590
                     /locus_tag="VFQ35_12010"
     CDS             2186..>2590
                     /locus_tag="VFQ35_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931411.1"
                     /translation="MTQAVVTRGARLLWATMALLALACSSETATSSHFDPRGEVFVSP
                     RFTLEQCEVVESALAAWSNATNGDVAFQVRIGEGYPRIVPAEVGERIMGEFVPSERPE
                     IVLDTQKALDATALRNLTLHELGHAIGLPHIPE"
BASE COUNT          452 a          848 c          891 g          399 t
ORIGIN      
        1 gccggtgagc ttcggttccg cgcgactccc aagctcgatt tgagcgccat cggcgagccg
       61 ctgacccata acctccacgc cgagctcgcg ctcaccgagc tgcgggccaa ctcggtgagc
      121 gcggacagcg attgcccttc actccggagc ccgagcctta ccgtgcggct cgcgtcgagc
      181 gaacgtgacg ccgagctcct cgcgaagatc gcggatctga ggttcggctg gggcgacttc
      241 aaggctcgaa ccgatacgga gctcgaggtg ctgctacgcc gtccggacga agaagacgcg
      301 gccccgccga ctctctcgtt ctcgctgaag aaccgagacg tttcactcgc gagtgggact
      361 gggaaaaaga gcggctggga cgcttccctc ccgaacctcg gcgtcaccgg caagttggcc
      421 ctcggggaca agaacgctgg gtggatcgag atcgcgctcg atcgcttgca gggccgcatc
      481 ggaggttcgc gcgtcgcttc gcgactgcaa gcgcacgtca aggtggcaga gctcgatatt
      541 gctcgcaagc actctcgttt ctcagccgac gtcgagctcg gacccgccga cgtgcaaacc
      601 gagagcgatc acgtgcgcga ttggtgggcg cgaatcgcgc tcggctccgc gctcgtcagc
      661 gtgcagcgca acttggatct atcggcgata ttccgagcga atcttcgcga cgcgactccg
      721 ggcctggccg tgctcgccga cagcggctcc gtgccggatc tcatcgcgcg gaacttgaag
      781 ctcgagcagc tcgaggtgac ggggattctc cagcgccgtt gccgcctcac cgacttcatc
      841 atcacgcaag cgtcgggcgg tccgctcagc gctcgcggcc gcctcgacag cacgagtgag
      901 gtcacgcgcg cggcgctgct cttgagagtg aacggcctcg aggccatctc ggccggggtt
      961 gcactcggcc cagacgggac cggcgtcaca ccgctcgccg gagacgactg gctgcgcgaa
     1021 gagcgcgaga agatggagcg ggcggcacgt gaggtcctgg aagggccgtg cgcgagcgag
     1081 cacggcgcgt gcggcgacga ctcatagagc ccgcgaatgg atgagtggcg cgagcgcgag
     1141 ccgagaccgc ggcgacgagc cgtccggcgc agcgagagat cggctcgcgg tatgacttac
     1201 ccgttcgcgg gcacttagtc gtctagggcg cgcggaatgg ccgctctgca gcgatgcgga
     1261 acattcgcgc ggctcggtcc tcgctccagc gataaaggca aacgcgatcg agccgagcgg
     1321 tcacgctttg gagctcgcac gcccccgccc acgcgagccc ggcgcggcgc tcggccggcg
     1381 tcgcccggcg actcgggcga gcgacggtta cgtgggcttt gggggcgcgc tgctcgcgcg
     1441 gcgcgccggc tgcggcggcc agagggtcgc gcagggaggc gatgagcgac tcggttgctt
     1501 cgcgtccttc gctgagtagt gccgagagcg cgctgtatgc ccggcgcgcg cccatcggca
     1561 cgacggtacc gagcctcacc tcgatcgccg gccgcacgaa ttcctgtgag cgagcgtcga
     1621 gctccgcaag ggctaggtgt gccgcggctt caccgcaagg gccgaggaaa cagagcgtga
     1681 cgtgaacgtc ctctggctga aaacggcgaa aattcggagg cagcggcggc aactcgagca
     1741 cgaacgcgcc gtccaccggc agggcaaaga accaattctc tcgtgacatg actatcgggc
     1801 gcgacgctca gagtatcgcc attccgacgg gctgatctac ctgctgaccg tcgtccactt
     1861 cagtggccgc ttccagcttt acgacaagac aggaagtgag cgccgagcct gccgcagctc
     1921 cgtcgcgacg ccggggtcga agcgcttcgg ctcgggcgtt cgtgcgtcta ggtctgagac
     1981 gagggccagc gtcagagctc ccaagctgag acgcgctagg tgcgcagccg aggggatctc
     2041 gcggagtcgc gggaggcatg gcgctcgcaa tcgttcttcg cgcagagccg cgcgtcaccc
     2101 cgcagggtaa tcacgccggc cggtacgcac gatggtacga aagacgagcg cagcgcgctg
     2161 tcacgaacag cctggagcgg tgcgaatgac gcaagcagtt gtcactcgcg gcgcgaggct
     2221 gctttgggcg acgatggccc tgctcgcgct cgcttgctcg agcgaaacgg cgacgagcag
     2281 ccatttcgac ccgcgtggcg aggtgttcgt ttcgccacgc ttcacgctcg aacagtgcga
     2341 ggtcgtcgaa agcgcgctcg ccgcgtggag caacgcgacc aacggagacg tcgcgtttca
     2401 ggtgcgcatc ggcgagggat acccgcgcat tgtccctgcg gaagtcggcg agagaatcat
     2461 gggcgaattc gttccgagcg agcgccccga gatcgttctc gacacgcaga aagcgctcga
     2521 cgcaaccgcg ctcagaaacc tgacgttgca cgagctcggc cacgccatcg gactgcctca
     2581 tatccccgag
//
LOCUS       DASTKS010000581         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_93782,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000581 DASTKS010000000
VERSION     DASTKS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93782"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFQ35_12015"
     CDS             complement(<1..384)
                     /locus_tag="VFQ35_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HET9931412.1"
                     /translation="MYKLALAALGVVFGDIGTSPLYAMRECFHGEHAIDATQENVYGV
                     LSLVTWSLTLIVSIKYVAYVLRADNKGEGGILALMALSSSALRQSRLHVVLITLGVFG
                     AALLYGDGVITPAISVLSAVEGLQVA"
     gene            complement(602..>2347)
                     /locus_tag="VFQ35_12020"
     CDS             complement(602..>2347)
                     /locus_tag="VFQ35_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9931413.1"
                     /translation="SSGSGSTLFVEPREVTDLGNRLRVREAAVAREVARVLAELSGLL
                     AGLVGEVRAAFEACVEADALDALARFAIATRARPIDVADDDRMELSGARHPLLALAGG
                     EVVPNDIVLAGGKALVVSGPNAGGKTVALKCLGLFAWMARAGIPLPAREESHVGWFSR
                     VLADVGDEQSLVRSLSTFSAHVENLAHILNHAAPHVLVLLDEVAAGTDPEEGAVLAAA
                     VLEELTERGAAVAVTTHYERLKELAAEPGRLENASVGFDFERMAPTFRLTLGVPGASS
                     ALAVAARHGIPAPLLSRAQQLLPQASADRERIISELARERARVESERVAIEADRARYA
                     AREAELAAEREALHAEAREHIEREAQKLAQEVKVARAELLRARDQLKAERLTLAELKT
                     AEARVARVASDVAIGGRFARSVPNVGGQGLAASELRVGATVRVKRSGAIGTVLEPPNR
                     DEVHLRVGALRTREKLSGLELVTTKSTKAQPKSTKWRPAPKNDSLAQAQRTVDNTLDL
                     RGVRVEDAPDRLDAFLDRMLGEGEPVGFVLHGHGTGALRSKVREHLAGSSHIEHARAA
                     EPDEGGEAFTVFWVR"
BASE COUNT          368 a          789 c          795 g          395 t
ORIGIN      
        1 tgcgacttga agcccttcga ccgccgacaa aacggagatg gccggggtga tgacaccgtc
       61 gccatagagg agagcggctc cgaacacgcc gagcgttatc aatacgacgt gcagccggga
      121 ctggcggagc gcagagctcg agagcgccat gagggccagg atgccaccct cacctttgtt
      181 gtcggcgcgc agcacgtacg caacgtactt gatagagacg atcagcgtca gcgaccaggt
      241 tacgagcgag agcacgccgt acacgttctc ctgggtcgcg tcgatcgcgt gctcgccatg
      301 gaagcactcg cgcatggcgt acagggggct cgtgccgatg tcgccgaaga cgacgccgag
      361 cgcggccaga gcgagtttgt acatgttccc gtgcgagccg tgcgaatgcg gcctctcgct
      421 catcggcggc acgctggccg gtgccgcgtc gcgggtggga ttcacgcttg ggccgttcgc
      481 acttcggagc acttgcgcag gctgactcgc taccgcttca gccggggccg tactcgccgc
      541 ggggcgagaa gcaggttctg gcatgtccgg gcgctacgct agcaccaacc cgcgacgggg
      601 ctcaacgaac ccaaaaaacg gtgaatgcct cgccaccttc gtccggctcg gcggcgcgcg
      661 cgtgttcaat gtggctggag ccggcgagat gttcgcgcac tttgctgcga agcgctcccg
      721 ttccgtgccc gtgcagaacg aagccgaccg gctcgccctc cccgagcatg cgatcgagaa
      781 aagcatccag ccggtctggc gcgtcttcca cgcgaacgcc gcgcaggtca agggtattgt
      841 ccacggttcg ctgggcctga gcgagcgagt cgttcttggg tgcgggacgc cacttggtcg
      901 acttcggttg agccttggtg ctcttcgtcg tgacgagctc gagccccgag agcttctcgc
      961 gagttctcag cgcgccgacc cgcaggtgaa cctcgtcgcg attcggtggc tcgagtacgg
     1021 tgccaatcgc acccgaccgc ttcacgcgaa cggtcgctcc gactcgcagc tcggaagcag
     1081 cgagtccttg tccaccgacg ttcggcacgg agcgagcgaa gcgcccacca atggctacgt
     1141 cggacgcaac tctcgcgact ctcgcctccg ccgtcttgag ctcggcgagg gtgaggcgct
     1201 ccgctttcag ctggtcgcgg gcacgcaaga gctcggcgcg cgcgaccttg acttcttgcg
     1261 cgagcttctg cgcctcgcgt tcgatgtgct cgcgcgcttc ggcatgcagg gcttcgcgct
     1321 cggctgcgag ctctgcttcg cgggccgcgt agcgcgcgcg gtctgcttcg atcgcgacgc
     1381 gttcgctttc gacgcgagct cgttcgcgcg caagctccga gatgatgcgc tcgcgatcgg
     1441 ccgaggcttg cggcaagagt tgctgcgctc gagagagcag cggggccggg atcccgtgcc
     1501 gggccgcgac cgcgagcgcg cttgatgcgc ctggaacccc caaggtgagc cggaacgtgg
     1561 gtgccatgcg ctcgaagtcg aagccgaccg aggcattttc gaggcgccct ggctccgccg
     1621 cgagctcctt caaccgttcg tagtgcgtcg tgaccgcgac ggcggcccct cgctccgtga
     1681 gctcctccaa taccgcggcc gcgagcacgg ctccttcttc ggggtcggtg ccagccgcga
     1741 cctcgtcgag gaggacgagc acgtgcggtg ccgcgtggtt cagaatgtgg gcgaggttct
     1801 cgacgtgcgc gctgaaggtc gacagcgagc gcacgagtga ctgctcgtct cccacgtcgg
     1861 cgagtacgcg tgaaaaccaa ccaacgtgag actcttcgcg cgctggcagc ggtattcccg
     1921 cgcgggccat ccaggcgaat aggccgaggc acttgagcgc gaccgtcttt cctcccgcgt
     1981 tgggccccga gacgacgagc gccttgccgc ctgcgagcac gatgtcgttc ggcacgactt
     2041 cgccccccgc gagggcgagc agcggatgac gggctcccga gagctccatg cgatcgtcgt
     2101 cggccacgtc gatgggacga gcgcgcgtcg caatcgcgaa tcgggcgagc gcgtcgagcg
     2161 cgtccgcttc gacgcacgct tcgaaggctg ctcgtacctc gccgacgagc ccagcgagga
     2221 gcccgctgag ttcggcgagc acgcgcgcaa cttctctcgc gaccgcggcc tcgcgcactc
     2281 ggagccgatt gccgagatcc gtcacttcgc gcggttcgac gaacagggtg ctgccggagc
     2341 cgctcga
//
LOCUS       DASTKS010000582         6405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1433482, whole genome shotgun sequence.
ACCESSION   DASTKS010000582 DASTKS010000000
VERSION     DASTKS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433482"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..300)
                     /locus_tag="VFQ35_12025"
     CDS             complement(121..300)
                     /locus_tag="VFQ35_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931414.1"
                     /translation="MPRPRINKPVHLEVTGLAPLPHAIEEWERAVIEPVLARLFDAPK
                     ANATEPKETRDGEAH"
     gene            1046..1960
                     /locus_tag="VFQ35_12030"
     CDS             1046..1960
                     /locus_tag="VFQ35_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931415.1"
                     /translation="MIRTTTSSFNSADHLFDEGMEHWWAGHRREALAYFRRAIKTDPR
                     HADAHNHLGIASLDARKLKHAEQHFRAAVDGGERRVERDGKLVPWPILENRPYLRALG
                     NLALALAEQRKWPEALAIHQRMLELNPNDNQGVRYLIGVEHLQVGDNQGAIEAFDKCI
                     GEEVGCAFGLALARLRAFGSSADVGEPLLNGFAANRYVAPMLLGERWERLDAFHGTNM
                     AEPEWADDVVKAQANLWHAVPRGAEALRFWWSAPVVATWRRKLDDNMVKLKGLPISDE
                     RNAVVSDGLALRSEQTVRSLARSVLSSS"
     gene            2208..2777
                     /locus_tag="VFQ35_12035"
     CDS             2208..2777
                     /locus_tag="VFQ35_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931416.1"
                     /translation="MSQLHLEPGRVYRTRDLATWSANTPRLAKRLVEQGELVPLAHGL
                     FAHPKQSRFGPVPPLDEEVMRAFLDGGAFVFTGPERWNALGLGTTAVFAVPLVYNTKR
                     SGTFTFGKRRFVLRRVAFPENPPAEWFVVDLLEHVDQAAASPDDVTDVLTRALAHGKF
                     GRERLREMAKRYGSRATIARVERALRESA"
     gene            2777..3619
                     /locus_tag="VFQ35_12040"
     CDS             2777..3619
                     /locus_tag="VFQ35_12040"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET9931417.1"
                     /translation="MSFVHETPEEFTDLVQIIVGETGMGRSVIEKDYWVTHVLWALSI
                     SGLDVWFKGGTSLSKGFGGLIERFSEDLDLKIEQGSVSALPSVSSWKSEGTKATAERR
                     AFFEQLPKVLVVPGCTVEIEQETIDKSWRGANLRVLYPGLFLGELPSAISGNVRLEVG
                     SARVTPHVAQDLTSWIHEKLVASNVLAEFGDNRAKGVRCVHPLVTLLEKLDALHRRFP
                     NDKAVPAAFVRHYEDAARLIEGRAKLPRLNRYDNVRALADEMLAQKQIVAMPSAADPA
                     LRPC"
     gene            3826..6135
                     /locus_tag="VFQ35_12045"
     CDS             3826..6135
                     /locus_tag="VFQ35_12045"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET9931418.1"
                     /translation="MTAPITDLLAQLEAPGTFATRLRAPAGELDIEVPGVGPLNFPIT
                     ARLAQKLRNVARPSPFGLREQTLHDASVRDTWEIATSRVKIAARRWKPVLATYLRTLQ
                     ADLGFPDECEVEAVFDKLLIYEKGQFFKPHQDSEKDDEMVGTLVVILPSEYEGGAVTV
                     EHRGEKKVFRRVESQAKDLSLLAFYADCHHAVSPIKSGVRVALTYQLRLAKRSKVALP
                     NVRADAVERLTMAVREYFAVPIAKRYGQSEPAPPERFVYLLDHEYTQRSLSWSHLKNG
                     DRARVAALRAAAERLDCECFLALAEVHETWLCEDDYPRGRYGRRGWRYDEEDDESESD
                     EYELIELQDSNIELNHWLDVGGKHVEGIPGTISDDELHFMKPSRDMDPFKSEHEGYQG
                     NYGNTLDRWYHRAAFVMWPRTNGFALRAQASPQWAVGELLAVPRTSATELESRVSSLL
                     PRWKRTAGSVEGVQFFANLIKLAARIEDGALAREWLAPVGLHRLTSKPMRRGLAALVD
                     KHGFPWAKELLAEWAKHHHWGTPAWAPLVADLCADLQASKSKACEALAGWLLEREVKT
                     ALDRCVTAFKQPPPWLDLESLSDEAKHLAHALAAGAAISAHQVITDALASLLGGKQST
                     PLSFLAQLLEACLARSPALKARVVGSVLHRECAERLGAILRAPARAESDWTITYPLSC
                     ACADCKELSQFLRSGRADCDWPLNKDRRQHIHQTLDSAKLPVLHTTLRRGSPHVLQLR
                     KDRSLFSRERAYRARVKEIFGALPAARSA"
BASE COUNT         1155 a         2131 c         2066 g         1053 t
ORIGIN      
        1 atgaccgcgt cgtggtgcgc ggcgaactcc tcggcggaat gccgctgtgc cgtgagtgac
       61 agttccttct cggcctgctc caccgtgctg acgcggagat agctcaccca gcgagtgcgt
      121 tcagtgcgct tcgccatcgc gcgtctcctt tggctcggtg gcgttcgcct tcggcgcatc
      181 gaagaggcgg gcgagcacgg gttcgatgac ggcacgctcc cactcctcga tcgcgtgcgg
      241 gagcggcgcg aggccagtga cttcgaggtg aacgggcttg ttgatgcggg gtcggggcat
      301 ggtcgtgtcc ttttgagaag gaaacgcggt cttccctgcc aaaacgggac acgaaatcga
      361 ccggccggat tcgcttgcga aatcgcgccg agcaggacgg tccggccctc acgctgcggg
      421 tgcgtccgcg agcgcgacgc caagccgtag acctgtcggt cgagcggtag gagcgtgcgg
      481 accaatcttg cagcgggccg ctgcagcacc gggcgacgca tgatcttcgg cagtcgcgag
      541 tacgggcttg acagcgtagt gctcatatga aaaaacaggg caccacatga gcttcgtgcg
      601 gcgcaagcgc ggccaggtcc tgctggtcca caacgagcga gtccccgggt cggcacgcat
      661 acgccagcgt gagctccatc gcttcacgtc gccctcagag atcgagcagg tacttgcacc
      721 cgccgagtgg gcgaagtgga cgaaggcgat ctcttggcgg gagcgcgacc tcgagttcga
      781 ctggccgtcg ctccgagagc gactgcaaac ggagttggcc acgtggaccg ccgagccagc
      841 aggagccact catcggcgca acaagaagat cgaacgcctc gcgtcggagc tcgttgtcga
      901 gctggcacca ctctcgcccg tgaagcccgc tgacgatgcg ctcgttgagc gtgttcgccc
      961 ttccttgctc gcgcttcgcg actctatcgc gaggctcctc gtttccgaac gccacgctgg
     1021 ttcccccgat cccaaggagt ccactatgat acgcacgacg acatcctcgt tcaactccgc
     1081 cgaccacctc ttcgacgagg gcatggagca ctggtgggct ggccaccggc gcgaggcgct
     1141 cgcgtacttc cggcgcgcaa tcaagaccga tccgcggcac gccgacgcgc acaaccacct
     1201 ggggattgcg agcctcgacg cgcgtaagtt gaagcacgcc gagcagcact tccgcgcagc
     1261 agtcgatggc ggtgagcggc gggtcgagcg cgatggcaag ctcgtgccgt ggccaattct
     1321 ggagaaccgg ccgtatcttc gggctctcgg aaatcttgcg ctggcgctcg ccgagcaacg
     1381 caaatggccc gaggcgctcg cgattcacca gcgcatgctc gagctcaacc cgaacgacaa
     1441 ccagggcgtg cgctatctga tcggcgttga gcacctgcag gtcggggaca accagggagc
     1501 catcgaagct ttcgacaagt gtatcggaga ggaagtcgga tgcgcgttcg gcttggcgct
     1561 tgcgcgactc cgcgcgttcg ggtcgtcggc agacgttggc gagcccctgc tgaatggctt
     1621 tgcagccaat cgctacgttg cgcccatgct cctcggtgag cgatgggagc gtctggacgc
     1681 ctttcacggc acgaacatgg ccgagcccga atgggccgac gatgtagtga aggctcaggc
     1741 caacctctgg cacgccgtcc cgcgcggcgc cgaagcgctc cgcttctggt ggagcgcgcc
     1801 cgtggtcgcg acgtggagaa ggaagctcga cgacaacatg gtcaagctca aggggctgcc
     1861 catctcggac gagaggaatg cggttgtctc cgacggcttg gcgctccgat ccgaacagac
     1921 cgtgcgcagc ctcgctcgct cggttctctc atcatcgtag gtgtcggcgc cgcgggggag
     1981 gcacgctccg aatggcccgc gcttgtagcg agacagcgcg aacgcgggag agcgtcccgg
     2041 ccgaccgcac gcaaccacat ggattcgctg gcgttcgcat tttcgggacg ccgcgagccg
     2101 agcgattccc tgagacggga ctcgaaggct gtcgcgagct gtgacgctca tgaccttgcc
     2161 gagtgcgatg ccttcgtgta tcttttattc gggaaattga atacgcgatg agccagctcc
     2221 acctcgaacc cggccgcgtg taccgcacgc gggacttggc cacctggagc gcgaacacgc
     2281 cccggctcgc caagcgcctc gtcgagcaag gcgagctagt gccgctggcc cacgggctct
     2341 tcgcgcaccc gaagcagagc cgcttcggcc cggtgccgcc gctcgacgag gaggtcatgc
     2401 gcgccttcct cgacggcggg gccttcgtct tcaccgggcc cgaacgctgg aacgccctag
     2461 gcctcggcac caccgccgtc ttcgcggtgc cgctcgtcta caacaccaag cgctcgggca
     2521 ccttcacgtt tggcaagcgg cgcttcgtgc tccgacgcgt ggcgtttccc gagaacccac
     2581 ccgccgaatg gttcgtcgtc gatctgctcg aacacgtcga ccaggccgcc gcgtcgcccg
     2641 acgatgtcac cgacgtgctc actcgcgcac tcgcccacgg caagttcggt cgcgagcgtc
     2701 tgcgcgagat ggcgaagcgc tacggctcac gcgcgacgat cgcccgcgtc gagcgcgcgc
     2761 tgcgggagag cgcgtgatga gcttcgtcca cgagacgccc gaagagttca ccgacctcgt
     2821 ccagatcatc gttggtgaga ccggcatggg tcgctcggtc atcgagaagg actactgggt
     2881 cacccacgtc ctctgggcct tgtcgatctc tggtctcgac gtctggttca agggcggtac
     2941 ctcgctctcc aagggcttcg gcggcctcat cgagcgcttc tccgaggatc tcgacctgaa
     3001 gatcgagcag ggctcggtga gtgctttgcc gagtgtgtcg agttggaaaa gtgaggggac
     3061 caaggcgacc gccgagcgac gcgccttctt cgagcagctc ccgaaggtgc tcgtcgtgcc
     3121 cggctgcacc gtcgagatcg aacaggagac catcgataag tcgtggcgtg gcgcgaacct
     3181 ccgcgtgctc tacccgggct tgttcctagg tgagctaccc agcgcgatct ccggcaacgt
     3241 gcgcctcgag gtcggcagtg cccgcgtcac gccgcacgtc gcgcaggacc tgacgtcgtg
     3301 gattcacgag aagctcgttg ccagcaacgt gctcgcggag ttcggcgaca atcgcgccaa
     3361 gggcgtgcgc tgcgttcatc ccctcgtgac gttgctggag aagctcgacg cactccaccg
     3421 caggttccca aacgacaagg cggtgccagc cgcgttcgtg cggcactacg aggacgccgc
     3481 gcgcctgatc gaggggcgcg cgaagctgcc gcgactcaat cgctacgaca acgtacgtgc
     3541 gctcgctgac gagatgctcg cgcaaaagca gatcgtggcg atgccgagcg ccgccgaccc
     3601 agcactgagg ccctgctgat gacgcccgct ggaccgcgat tcgggaggcg cacgcgacga
     3661 tcggcgcgat gtactggggc ccgcgtctct ccgtcgagga cgcgaccgcg acgatccgcg
     3721 cgtggatcac tgccgaactc ggctgagacg ctgttgcctt cgctcacgag cgccgaacca
     3781 ccggccacgg tagcaatgct agaacccacg ccgagacgtg acgacatgac cgcacccatc
     3841 acagacctgc tggcccagct cgaagctccc ggaacgttcg cgacacgact gcgcgcgccc
     3901 gcgggtgagc tcgacatcga ggtaccgggc gtgggcccac tcaacttccc gatcaccgcg
     3961 cgcttggctc aaaagcttcg caacgtggcg cgcccttccc ccttcggctt acgcgagcag
     4021 actctgcacg atgccagcgt gcgagacact tgggagattg ccacgagccg cgtgaagatc
     4081 gctgcgcgtc gttggaagcc cgtgctcgcg acgtacctgc gaacactcca ggccgacctt
     4141 ggttttccag acgaatgcga ggtcgaggct gtattcgaca agctcctcat ctacgagaaa
     4201 ggtcagttct tcaagcctca tcaagactcg gaaaaggatg acgaaatggt gggcacgctc
     4261 gtcgtcatcc ttccgtcgga gtacgagggt ggcgccgtga ccgtggagca ccgcggggaa
     4321 aagaaggtgt tccggcgcgt cgagagccag gcaaaggacc tctcgctcct agcgttctac
     4381 gccgactgcc accacgcagt gagcccgatc aagagcggtg tccgcgtggc gctcacctat
     4441 caactgaggc tcgcgaagcg ctccaaggtg gccctcccca acgttcgagc cgacgcggtc
     4501 gagcgcctga caatggccgt gcgggagtac ttcgccgtac ccatcgcgaa gcgctacgga
     4561 cagtccgagc ccgcgcctcc cgagcgattc gtctacctgc tcgaccatga gtacacgcaa
     4621 cgaagtctct cttggagcca cctgaagaac ggcgaccgag cccgcgtcgc ggcgctgcga
     4681 gccgcggccg agcggctcga ctgcgaatgc ttcctcgcac tcgcagaggt ccacgagacg
     4741 tggttgtgcg aggacgacta cccaaggggc cgatacgggc gccgcggctg gcgttacgac
     4801 gaggaggacg acgagtcgga gtcggatgaa tacgagctca tcgagctcca ggacagcaac
     4861 atcgaactca accactggct cgacgtcggc ggcaagcacg tcgaaggaat tccaggaaca
     4921 atcagcgacg acgagcttca cttcatgaag ccttcacgcg acatggatcc gttcaagagc
     4981 gagcacgagg gctatcaggg caattacggc aacaccctcg atcgctggta ccaccgagcc
     5041 gcgttcgtga tgtggccacg cacgaatggg tttgcgctcc gggcacaggc gtcgccccag
     5101 tgggccgtgg gcgagctgct ggctgtgccg cgcacaagcg cgacagagct cgagtcgcga
     5161 gtaagctctc ttctgccccg atggaagcgc acggcgggca gcgtcgaggg cgttcagttc
     5221 tttgcgaacc tcatcaagct cgcggcacgc atcgaggacg gcgcactcgc gcgcgaatgg
     5281 cttgcgccag tgggactgca ccgcctcacc agcaagccga tgcgccgcgg tctggcagcc
     5341 ctggtcgaca agcacggttt cccgtgggcc aaggagctgc tcgcggagtg ggcaaagcac
     5401 catcactggg ggacacccgc gtgggcgccg ctggtcgccg acctctgtgc ggacttgcag
     5461 gcgagcaaga gcaaggcatg tgaagcgctg gccggctggt tgctcgagag ggaggtcaag
     5521 accgctctgg accgctgtgt cacggctttc aagcagcccc cgccgtggct cgatctggag
     5581 tccctcagcg acgaagccaa gcaccttgcc cacgcgctcg ctgcgggagc agccatctcc
     5641 gctcaccagg tcatcacaga cgccctcgcc tctctgctcg gcggcaagca aagcactccg
     5701 ctgtcctttc ttgcgcagct gctcgaggcc tgcctcgcca gatccccggc tctcaaggcg
     5761 cgggtcgtcg ggtccgtcct ccatcgtgag tgcgcagagc gcctcggcgc gatcctgcgc
     5821 gcaccggcac gggcagaaag cgattggacc atcacctacc cgctgagctg cgcctgcgct
     5881 gactgcaagg agctctccca gtttctacgc tcaggccgcg ccgactgcga ttggccgttg
     5941 aacaaggatc gacgccagca tatccaccag acgctcgatt cggccaagct gcccgtgctg
     6001 cacacgacgc ttcgacgcgg aagcccgcat gtcctgcagc tacgcaaaga ccgctcactc
     6061 ttttcgcgtg aacgagctta tcgagcgcgc gtgaaagaga tcttcggcgc actcccagcc
     6121 gcgcgctcag catgagccac catcatcagg aatcggaagg tggaccgcga gcgcgcgccg
     6181 gggctccgca ttcgtcgcgg gacagcgcga atcggagaga gcgtcccggc ccacgtcgcg
     6241 caaccacctg gattcgcaag cgttcgcatt ctcgggaccg tgcgagccaa gcgaacccgt
     6301 cccgccgaga gctccgcgaa cgggttagca ggcggcgccg aatgcggagc cagtttcgag
     6361 tcggcgcaat gcagtcgacc caacgttgaa cggaacccgg agcgg
//
LOCUS       DASTKS010000583         7901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_540262, whole genome shotgun sequence.
ACCESSION   DASTKS010000583 DASTKS010000000
VERSION     DASTKS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540262"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3173)
                     /locus_tag="VFQ35_12050"
     CDS             complement(<1..3173)
                     /locus_tag="VFQ35_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931419.1"
                     /translation="MPPLRPSKNSEPPPPDRTASAAPVPPKSGPSKAPAPSRAPSSKG
                     SNPNASSAPLSKVPPAPKRSVAPADPPSSPSASGGKSLRPPVPAGRSAVPLPPPKPSS
                     LRAPPRPPVRNDHPSLADDDIEEASDDAPTRGLGRPLPKPPSPPLRGGSTPPGQVNIT
                     PSAPPVRAGTVDAPTIALPKKHFPEVTIDATTLLRLGGPTPSVGQPPSAPQGSREQAA
                     RELLTALKTELDQHPKALRAGRLHFECARLLESPIGALKEAADHYLKAHALLGDHLPS
                     IRGARRVLLTLGRAQEAVQLFEAELKLTADAEHKAQLLYDKASVQEDMLGQRKEAREA
                     LEAASELSRGDATRVKAIERAEALARGWDGLGRAYEREANAIKDDPRHRAVLVAARAR
                     LLEAHRGDPATAVELYQNAAESDPESSTALLALKRLHYGHQRWADLIGVLEREAELAS
                     DSSVRAMAYYRVGRVWVDRLGNVDKALGSLEKAAEQSAVDPMILEELARLYEVAGRAA
                     ELANMLERLAEQVSSDSDKLAYFLRIGELYQVRLKNEAQAIAWYERARAIDRCYIPAL
                     QALSELYTDKGEFDKLVQVHAGEAEEALDPTRRAAAHARIAEIYESRLGRPDKALEPH
                     ARALGVLPGFSTSFKAYARLLMQGKRYPELVELYERAVDQASDNEGKLTYLYKIGRLY
                     EDALGSPAQAMVAYKRILEIAPHEVDAIHSLQRAADRAGEYKELISALELEAERLPDK
                     RRKLELLHRAGEVAEIDLGDDALSVSLYKRITEIDKAYAPAYASLGRLYHKAGRWEEL
                     LETYKNELRLLNKGPMLSALQFKMGQIYEDKLARDEDALASYRRAVEADPSHRAAMRA
                     LERKLEQKGNWDELVRVLEAELVILEDAKLKARTWLRIGEVAESRLRAQDRALSAYEQ
                     ALVLDSELSPARDGRLRLLTEARDYRHLVEELEREALSQKDVRLSVAALLRAGEVYRD
                     DLSEPSRAVRAFEQVLERDPAHIEALLALEALYAERGAWDALASIYSTEARVLSDKAA
                     RVAALRELGRLQQGGKVESKDRGRQAFV"
     gene            3383..4693
                     /locus_tag="VFQ35_12055"
     CDS             3383..4693
                     /locus_tag="VFQ35_12055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9931420.1"
                     /translation="MPTDLLVITAARAIDPASGLDAIVDIVVESGKISRVGPGAAQGL
                     AHSERARLIDARGRWVLPAFVDLHAHLREPGQEYKEDIRSGLAAAAAGGYAHVCVMPN
                     TRPVNDTRAVTELMLRRAEECGGPSLHPIAAITMGQKGKELTEMAELRDAGAVGVSDD
                     GVCVMSSAVMRRALEYAKNFDMPVIQHAEDHDLTQGADMHEGMVSAKLGLRGWPRVAE
                     DVIVARDVILAEYVGARYHLAHVSTAGAIRIVREAKHRGIDVTCEVTPHHLLLTDESV
                     IGYDTACKVNPPLREPEDVAALRAALADGTIDAVATDHAPHSRLEKDCEFSEASPGMM
                     GLELCFGLLLGLVGADGLSLPRLVEAMSTRPARIAGVEAPRLREGAIAEFVLFDPEAR
                     WKPRERKLETKSKNSPFMDRELKGKILMTLARGEVVFDTLGSER"
     gene            4690..5820
                     /gene="carA"
                     /locus_tag="VFQ35_12060"
     CDS             4690..5820
                     /gene="carA"
                     /locus_tag="VFQ35_12060"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET9931421.1"
                     /translation="MSDRKGHLALADGTVLPGTLFGADGTATGEVVFTTGMTGYQEVL
                     TDPSYTGQIVTMTAPQIGNTGINEQDPESVDGKPQVAGFIVRDDSVTVSNWRSKETLD
                     AYLARQGVVAISGVDTRRLTRYLRDKGSQNGAIGSGPVEELVERARRAPSMEGLDLVA
                     RVSPKQRYVFSESRAEWNLAAAPASGEQPLHVVAVDYGAKRNIFRCLVDTGFKVTVVP
                     ATTSASDILAQNPDGIFLSSGPGDPAAVTYAVDTIKGLHGKKPMFGICLGHQLLALSL
                     GARTYKLKFGHRGLNQPVKNLATGQVEITTQNHGFVVDVDSLKGMAKSTHLHLNDGSS
                     EGLEVPELKAFSVQYHPEAAAGPHDALYLFDRFKKLMLDARK"
     gene            5817..7256
                     /gene="rimO"
                     /locus_tag="VFQ35_12065"
     CDS             5817..7256
                     /gene="rimO"
                     /locus_tag="VFQ35_12065"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9931422.1"
                     /translation="MTEPSNPDRTIHFVSLGCPKNRVDTEVMLGLAERTGYTHVDDPE
                     SARVIVVNTCGFIDAAKKESIDTILELAQHKQSGSCERLVVTGCLSQRYPEELARDLP
                     EVDHLLGSSDMLKLEQVLKGGAERVLVGHPAEWVVAASDPRRVSTRGRSAYVKIAEGC
                     NRTCSFCVIPQIRGKQRSRSADDVVREVEALAAAGVLEVNLVSQDTIAYGRDLSDSKG
                     LADLVRRVAEVKGIRWVRVFYLYPEKLDDELIELLGQHPVVLPYVDMPLQHVADGMLR
                     RMRRGHGGKRLYELVERLKTRIEGLTMRTAFIVGHPGETDEEFEELMAFVRHSEFDRV
                     GIFQYSDEPTARAYELSDKVSDKVAESRLKKLTRLQRGISKKRNRALVGRTLEVLVEG
                     PSEDSEFVMVGRHAGQAPEIDGNVYLSGGEVLPGELRRVEVTQATDYDLVGEVFEDEA
                     PLVPAPSPRRTAQLAHRSSDGRRVSLKTV"
     gene            complement(7278..7637)
                     /locus_tag="VFQ35_12070"
     CDS             complement(7278..7637)
                     /locus_tag="VFQ35_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931423.1"
                     /translation="MQGARLTPLAVSASRRTILVAIAAIAATSCLSPTLPLPPPSDPK
                     QSDIDETTGTLHLTGHVRPTSWIYALNLETYKGYIQITGADGRYDLTVAASHGDRFSL
                     WYDYRGDQSDSLEITIK"
BASE COUNT         1323 a         2634 c         2629 g         1315 t
ORIGIN      
        1 acgaaggctt ggcggccccg atccttggac tcgaccttac cgccttgctg cagccgaccg
       61 agctcgcgca gcgcggcgac gcgcgccgcc ttatcggaga gcacgcgcgc ttcggtcgag
      121 tagatcgacg cgagcgcgtc ccaggcgccg cgctcggcgt acagcgcttc gagcgcgagc
      181 agggcctcga tgtgggcggg gtcgcgctcg agcacttgtt cgaaagcgcg caccgctcgc
      241 gaaggttcgc tgaggtcgtc tcgatagact tcacccgcgc gcagcaacgc cgcgaccgag
      301 agccgaacgt ccttttgcga gagcgcctcg cgctcgagct cctcgaccag gtgccgatag
      361 tcgcgcgcct cggtcagcaa gcgcagtcgt ccgtcgcgcg ccggcgaaag ctcggagtcg
      421 agcacgagcg cctgctcgta tgcgctgagc gctctgtctt gcgctcgcag ccggctctcg
      481 gcgacttcgc cgattcgcag ccaggttcgc gccttgagct tcgcatcctc gaggatgacg
      541 agctcggcct cgagcacgcg cacgagctcg tcccagttgc ccttctgctc tagcttgcgc
      601 tcgagggccc gcatcgcggc tcgatgactg ggatccgcct cgacggcgcg acggtaagag
      661 gcgagcgcgt cttcgtcgcg agcgagcttg tcctcgtaga tttgccccat cttgaactgg
      721 agcgcggaga gcatcggccc cttgttgagc aggcgcagct cgttcttgta ggtttcgagc
      781 agctcctccc agcgcccggc cttgtggtag aggcggccga gcgatgcgta cgccggcgcg
      841 tacgccttgt cgatctcggt gatgcgcttg tagaggctca ccgacagcgc gtcgtcgccg
      901 agatcgattt cggcgacttc gccagcgcgg tgcaagagct cgagcttacg acgcttgtct
      961 gggagacgct cggcctcgag ctcgagggcc gagatcagct ccttgtattc gcccgctcga
     1021 tcggccgcgc gctgcagcga atggatcgcg tcgacctcgt ggggcgcgat ctccaagatg
     1081 cgcttgtagg cgaccatggc ttgcgccgga gagccgagcg cgtcctcgta cagccgccca
     1141 atcttgtaga ggtaggtgag cttgccctcg ttgtcgctcg cttgatccac ggcgcgctcg
     1201 tagagctcga cgagctctgg gtagcgcttg ccctgcatca agagccgcgc gtacgccttg
     1261 aacgacgtgg agaagccggg cagcacgccg agagcgcggg catggggctc gagcgctttg
     1321 tcggggcggc cgagcctcga ctcgtagatc tccgcgatgc gagcgtgcgc ggccgcgcgg
     1381 cgcgtcggat ccagcgcctc ctcggcttcg cccgcgtgca cttgcacgag cttgtcgaac
     1441 tcgcccttgt ccgtgtagag ctcggagagc gcctggagcg cggggatgta acaacggtcg
     1501 atcgcgcggg cccgctcgta ccaggcgatc gcttgcgcct cgttcttgag tcggacttgg
     1561 tagagctcgc cgatgcgcaa aaagtacgcg agcttgtccg aatccgagct cacttgctcg
     1621 gccaggcgct cgagcatgtt ggcgagctcc gcggcgcgcc ccgcgacctc gtacaggcgc
     1681 gcgagctcct ccaagatcat cggatcgacc gcgctctgct cggccgcctt ctcgagcgac
     1741 ccgagcgcct tgtcgacgtt tcccaaccgg tccacccaaa cccgcccgac gcggtagtac
     1801 gccatcgcgc gcaccgacga gtcactcgcg agctctgcct cgcgctcgag cacgccgatg
     1861 agatctgccc agcgctggtg accgtagtgc aggcgcttga gcgcgagcaa cgcagtggag
     1921 ctctccgggt cgctctccgc cgcgttctgg tatagctcga ccgcggttgc cggatcgccc
     1981 cggtgcgctt cgagcaagcg cgcccgtgcc gccaccaaca ccgcgcggtg ccgcgggtcg
     2041 tccttgatcg cgttggcttc gcgctcgtag gcgcgcccga ggccgtccca cccccgcgcg
     2101 agcgcctctg cgcgctcaat cgccttcact cgtgtcgcgt cgcctcggct gagctcgctc
     2161 gcggcttcga gcgcctcgcg cgcctccttg cgctgcccga gcatgtcctc ttgaacggaa
     2221 gccttgtcgt agagcagctg ggctttgtgt tccgcgtcgg cggtgagctt gagctcggct
     2281 tcgaacagct gcactgcctc ctgcgcgcgc ccgagagtga gcaacacgcg gcgcgctcca
     2341 cggatggacg gcaggtgatc gccgagcagc gcgtgcgctt tcagatagtg atcggcggct
     2401 tccttcaacg cgccgatcgg tgactcgagc agccgcgcgc actcgaagtg caggcgtccg
     2461 gcgcgcagcg ctttcggatg ctgatcgagc tcggtcttga gggcggtgag gagctcgcgc
     2521 gcggcctgct cgcgagagcc ctgcggcgcc gaagggggct ggcccaccga gggcgtaggc
     2581 cctccgaggc gaagcaaggt cgtcgcgtcg atggttactt cggggaaatg cttcttcggc
     2641 agcgcaatgg tcggcgcatc gaccgttccc gcgcgcacgg gcggagcgct cggcgtgatg
     2701 ttcacttgcc cgggcggcgt gctcccgccg cgcaacggcg gcgacggagg ctttggcaaa
     2761 ggacggccga ggccgcgggt cggtgcatcg tcgctcgctt cttcgatgtc gtcgtccgcg
     2821 agcgatgggt gatcgttgcg cacgggcggg cgcggcggcg ctcgcaggct gctcggcttt
     2881 ggcgggggca aaggcacggc ggagcggccc gcgggcaccg gaggacggag actcttgccg
     2941 ccgctcgcgg aaggcgagct cggcggatct gccggcgcga cggagcgctt cggcgcgggc
     3001 ggcactttgc tcagcggagc gctcgaagcg ttcgggttcg agcccttgct gctcggcgct
     3061 cgcgacggag ctggtgcctt ggatggcccg ctcttgggcg gcacgggagc agcgctcgcg
     3121 gtccggtcgg gaggcggcgg ctcgctgttc ttggagggac ggagaggcgg cacgctcgac
     3181 cggcctcctt cagggcgcgg tgggctcgcc ttcttttggc tcttcgggtc gtcagccatg
     3241 cgatatgccc ggcgtttggc gcgagaatgc cggttacaaa gcagcgtaac atcactggta
     3301 gggtttcagc acgcccgtac ttctgccgcc ttgcacgggg accctgcacc ggccacctgc
     3361 cccatgctat caagcgcccg ccgtgccgac cgaccttctc gtgatcaccg ccgctcgcgc
     3421 catcgatccc gcctcgggac tcgacgcaat cgtcgacatc gtcgtcgaat ccggaaagat
     3481 cagccgcgtc ggccccggag ccgcccaggg cctcgcacat tcggaacgag cccgactcat
     3541 cgatgccagg ggccgttggg tgcttcctgc cttcgttgac ctacacgcgc acctgcgtga
     3601 gccagggcaa gaatacaaag aggacattcg aagcggcctc gccgctgcgg ccgccggcgg
     3661 ctacgcgcac gtctgcgtga tgccgaacac tcgcccggtg aacgacacgc gcgcggtgac
     3721 ggagctcatg cttcgtcgag ccgaagagtg cggcgggccg agccttcacc ccatcgcggc
     3781 gatcacgatg ggtcagaagg gcaaggagct caccgaaatg gccgagctac gcgacgccgg
     3841 agcagtcggc gtcagcgacg acggcgtttg cgtgatgtcg agcgcggtga tgcgacgcgc
     3901 cctcgagtac gcgaagaact tcgacatgcc ggtgattcag cacgccgagg accacgacct
     3961 gacccaaggc gccgacatgc acgagggcat ggtctcggcg aagctcggct tgcgcggctg
     4021 gccgcgcgtg gccgaggacg tcatcgtggc ccgcgacgtg atcctcgccg agtacgtcgg
     4081 tgcgcgctac cacctcgcgc acgtctcgac cgcgggcgcc attcgcatcg tgcgcgaagc
     4141 gaagcaccgg ggcatcgacg tgacgtgcga agtgacccct caccacctgc tactcacgga
     4201 cgagtcggtc atcggctacg acaccgcctg caaggtgaac ccacccctgc gcgagccaga
     4261 agatgtggcg gctttgcgcg ccgccctagc cgacggaacc atcgacgcgg tcgccaccga
     4321 tcacgcccca cattctcgcc tcgagaaaga ctgtgaattc tcggaggcct cgccgggcat
     4381 gatgggactc gagctctgtt ttgggctatt gttgggcctc gtcggcgccg atgggctcag
     4441 cctgccccgc ctcgtcgaag cgatgtcgac ccgcccggct cgcatcgcgg gagtcgaggc
     4501 acccaggctg cgcgaaggcg ccatcgcaga gttcgtgctg ttcgatccag aggcacgctg
     4561 gaagccgcgc gagcgaaagc tcgaaaccaa gagcaaaaat tcgccgttta tggacagaga
     4621 gttgaaagga aagattctga tgacgcttgc gcgtggagag gtggtcttcg acaccctcgg
     4681 gagtgagcga tgagcgaccg caaaggtcac ctcgccctcg cggacggcac cgtgctgccg
     4741 ggaacgcttt tcggcgcgga tgggaccgcg accggcgaag tggttttcac cacgggcatg
     4801 actgggtacc aagaggtcct cacggatccg tcctacaccg gacagatcgt cacgatgacc
     4861 gcgccccaga tcggcaacac cggcatcaac gagcaagatc ccgagagcgt ggacggcaag
     4921 ccccaggtcg ccggcttcat cgtgcgcgac gacagcgtca ccgtctcgaa ctggcgctcc
     4981 aaggagacgc tcgatgcgta cctcgcgcgt caaggcgtgg tcgcgatctc cggcgtcgac
     5041 acgcgccgac tcacgcggta cctgcgcgac aagggttctc agaacggcgc catcggcagc
     5101 ggcccagtcg aagagctcgt cgagcgagcg cgtcgggccc cgagcatgga gggcctcgat
     5161 ctcgttgctc gggtctcgcc caagcagcgc tacgtgttca gcgaatcgcg cgcggagtgg
     5221 aacctcgccg ccgcgccggc gagcggagag cagccactgc atgtggtcgc cgtcgactac
     5281 ggcgccaagc gcaacatctt ccgttgcctg gtggacacgg ggttcaaggt tacggtggtg
     5341 cccgccacga cgagcgcgag tgacatcctc gcccaaaacc ccgacggcat cttcctctcg
     5401 agcggccccg gggatccggc cgcggtcacg tacgcggtcg acaccatcaa gggcctgcac
     5461 ggcaagaagc cgatgttcgg catctgcctc ggacatcagc tcctcgcgct gtccctcggc
     5521 gcgcgcactt acaagctgaa gttcggccac cgcggcttga accagccggt aaagaacctc
     5581 gccaccggtc aagtcgagat caccacccaa aaccacgggt tcgtggtgga cgtggactca
     5641 ttgaagggca tggccaagtc gacgcatctt cacttgaacg atgggtcgag cgaagggctc
     5701 gaggtgcctg aattgaaggc cttcagcgtg cagtaccacc ccgaggcagc agcggggccg
     5761 cacgacgcct tgtacctctt cgatcgcttc aagaagctca tgctcgacgc gcgcaaatga
     5821 ccgagccgtc gaaccctgac cgcacgattc acttcgtgag cctcggttgt cccaagaacc
     5881 gagtcgacac cgaggtgatg ctgggcttgg ccgagcgcac cggctacacc cacgtcgacg
     5941 atcccgagtc cgctcgcgtc atcgtcgtga acacgtgcgg cttcatcgac gccgccaaga
     6001 aggagtcgat cgacactatc ttggagctcg cgcagcacaa gcagagcggc agctgcgagc
     6061 gcctcgtcgt cactggctgc ctcagccagc gctaccccga ggagctcgcg cgcgaccttc
     6121 ccgaggtcga tcacttgctc ggctcgagcg atatgttgaa gctcgagcag gtgctgaagg
     6181 gcggtgccga gcgcgtactc gtgggacacc ccgcggagtg ggtcgtagct gcgagcgacc
     6241 ctcgccgcgt ctcgacgcgc ggccgcagcg cctacgtgaa gatcgccgag ggctgcaacc
     6301 gcacctgctc attttgcgtc atcccgcaga tccgcggcaa gcagcgctcg cgcagcgccg
     6361 acgacgtcgt acgcgaggta gaagcgctgg cggccgccgg agtcttggag gtgaacctcg
     6421 tctcacaaga cacgatcgcc tacggccgcg acctctctga ttcgaaaggc ctcgccgacc
     6481 tcgtgcggcg cgtcgccgag gtgaagggca tccgctgggt gcgcgtcttc tacctctacc
     6541 cggagaagct cgacgacgaa ctcatcgagc tcctcggtca acacccggtc gtgctccctt
     6601 acgtcgacat gccgctccag cacgtcgcgg acggcatgct gcgtcgtatg cgtcgcgggc
     6661 acggcggcaa gcgcctctac gaactagtcg aacgactgaa gacccgcatc gaaggcctga
     6721 cgatgcgaac cgccttcatc gtcgggcacc ccggtgagac ggacgaagag ttcgaagaac
     6781 tgatggcttt cgtgcggcac tccgagttcg atcgggtcgg catctttcag tactcggacg
     6841 agccgaccgc gcgtgcctac gagctctccg acaaggtctc tgacaaggtc gccgagagcc
     6901 gcttgaagaa gctcacacgg ctccagcgcg gaatcagcaa gaagcggaac cgagccctcg
     6961 tgggacgcac gctcgaggtg ctggtcgagg gccccagcga agacagcgag ttcgtgatgg
     7021 tcggccgcca cgcgggacaa gctcccgaga tcgacggcaa cgtctacttg tctggcggag
     7081 aagtcctccc cggggaactt cggcgggtcg aggtgactca agccaccgac tacgatctcg
     7141 tcggcgaagt tttcgaggac gaggcgccgc tcgttcccgc accatcccca cgccgtaccg
     7201 cccagctcgc acatcgcagc agcgatggcc gccgcgtcag cttgaagacc gtttgagcgc
     7261 gagttcgcgc ccgggcatca cttgatggtg atctcgagcg agtcgctctg atcgcctcgg
     7321 tagtcgtacc agagagagaa gcgatcgccg tgcgaggccg cgacggtgag gtcgtaacgc
     7381 ccgtcagcgc cggtgatctg aatgtagccc ttgtaggttt cgaggttcag cgcgtaaatc
     7441 caggaggttg gcctgacgtg accggtgagg tggagagtcc ccgtggtttc gtcgatgtcc
     7501 gactgcttcg ggtcgctcgg tggaggcagc ggcagcgtcg gagaaaggca cgacgtcgcc
     7561 gcgattgcag cgatggcaac caagatggtg cggcgcgacg cggacacggc cagtggcgtc
     7621 aacctagcac cctgcatgcc gctctcaggc gagccctgct ggcgcaaaat ccggcgcggc
     7681 tgacacccgg ctgagcagag caggaacgag tcattggcaa cacagtttac gctcgagaca
     7741 actaagttgc caaccaccgg aggcgggccg cgtgacgcgc gtcgcgcgac caccgacctc
     7801 gtcttgcacg aaagcactgg ctcggaggtc ggacgactgt agagcgcacc tggttcggca
     7861 aaaagcgtac gaaaaatggc ccactcgaga gccaggtgtt a
//
LOCUS       DASTKS010000584         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1209180, whole genome shotgun sequence.
ACCESSION   DASTKS010000584 DASTKS010000000
VERSION     DASTKS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209180"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..928)
                     /locus_tag="VFQ35_12075"
     CDS             complement(83..928)
                     /locus_tag="VFQ35_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9931424.1"
                     /translation="MPELPEVETVCRTIRHALQGQRIARVEVVRDAIVFGSASPKTIE
                     AALLGRTVKAVGRRGKFFWLCLSGEGPTVFGHLGMSGWIREVGLEGVRLHGHGDAPFD
                     DEHGRPRFLKLGVAARSGRGIALTDPRRLGRVWLGPTPEEDRRVKRLGPDAFEALPPA
                     KELLALFARRKIPIKAVLLDQSALAGIGNWIADEVLYQSRIAPHRVAASLTPVEVAAL
                     RRAIVSVLARAVKVGADHKKYPKSWLFEHRWGGSRGAQQIGGQRIVREEVGGRTTAWV
                     PTRQK"
     gene            complement(972..2654)
                     /locus_tag="VFQ35_12080"
     CDS             complement(972..2654)
                     /locus_tag="VFQ35_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9931425.1"
                     /translation="MKLGTILRLLLAMLGILYSARAVADPTASLEAPASATAGSKVAV
                     KWTGPAGMLDRIAALPAGSPDTTAPKGLPCYPAGSKDAQLRPAYVKLPEEPGEYELRY
                     FMGGKVLARRKISVRAATATIQAPATAVAGSRISVAWRGPNNEFDKLGIVKAGAPERT
                     AAAGTPNFTGRSSPMGVNVPEQPGEYEIRYLTGAGATLARANLTVTAASASISVAPSV
                     VAGNRFPVTWTGPAGEYDAIVIAKKGSPDRTSVAGDFTMKGSPLGLGAPVETGEYELR
                     YKTGATGTVLARATLRVIPGQSEPGFVKVTLSAKAAGPDSAFAPGAVEVVLDASGSML
                     QRIGSERRIDIAKHTLGELTGELIPPGTPFALRVLGRGADTCQSELDIPFAPLDRGAA
                     GSKIAALQAKGNARTPISAALERVSADLAAAKGERVVILVSDGEETCGGNPARTIQKL
                     VQSGLQVRVNIVGFAVDDDKLAAKFETWSHAAGGSYFDARDAKALSRAFSEAVRPTFE
                     LIDAQKNVIAKGIVGGDPVRVLPGTYSLALKGRKEPPRSVTVRSRETSAVTY"
     gene            2824..>3336
                     /locus_tag="VFQ35_12085"
     CDS             2824..>3336
                     /locus_tag="VFQ35_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931426.1"
                     /translation="MNRAARLPFGFVASLLVGLNLAACGNANPCPRVVTAQRTLPASA
                     VAAASAKPSAAHPIVPCTVTVDKPGSLLADFEKPNAGEAWFSYSDGTAGGVLRQETST
                     WKDGQRALHVTASGFREWGAGLGVPVGMLAAAQGPCEFDASAYSGVRFRARGRGALRL
                     AVASVPSMPVA"
BASE COUNT          547 a         1139 c         1049 g          601 t
ORIGIN      
        1 gatcctgaga gactcgctga gctcgctctc gagttctaat tctgcgcact tcgcggccca
       61 tcgatagttg cggccgtgct cgctactttt ggcgcgtggg gacccacgcc gtcgtgcgtc
      121 cgcccacttc ttctcgaacg atgcgctgtc cgccgatttg ctgggcgccc cgactgccgc
      181 cccagcgatg ctcgaatagc cacgacttcg ggtacttctt gtggtcggcg ccgaccttca
      241 ccgcgcgtgc gagcaccgag acgatggcgc gacgcaacgc cgccacctcg accggcgtga
      301 gtgaagccgc gactcgatgc ggcgcgatgc gcgactgata cagcacctca tcggcgatcc
      361 aattgccgat gccggcgagc gcactctggt cgaggagaac ggctttgatc gggatcttgc
      421 gccgcgcgaa cagcgcgagc agctccttcg cgggcggcag cgcctcgaac gcatccggac
      481 ccaagcgctt gactcggcga tcttcctccg gcgtcggccc cagccaaacg cggcccagtc
      541 gacgcggatc agtcaacgcg attccgcggc ccgagcgcgc cgcgacgccg agcttcaaaa
      601 atcggggccg tccatgctca tcatcgaacg gcgcgtcgcc gtgaccgtga aggcgcaccc
      661 cctccaaccc aacttcgcga atccacccgc tcattccgag gtgcccaaac acggtcgggc
      721 cttcgccgct caaacacagc caaaagaact ttccgcgtcg accgaccgcc ttcaccgtgc
      781 gtccgagcag cgccgcctcg attgttttcg gggaggcgct cccgaacacg atcgcgtcgc
      841 gaacgacctc gacccgcgcg atccgctgcc cttgcagcgc gtggcgaatc gttcgacaaa
      901 cggtctcgac ctcggggagc tcgggcatcc ctcgattgta gccgcccgct gggctaaggg
      961 ctctcgtgag tttagtacgt caccgcgctg gtctcccgtg agcgcaccgt gacagagcgc
     1021 ggcggctcct tgcgaccctt cagcgccaac gagtacgtgc cggggagaac ccgcactgga
     1081 tcgcctccca cgatgccctt cgcgatcacg ttcttttgcg catcaatgag ctcgaatgtg
     1141 ggtcgaaccg cctcgctgaa ggcacggctc agggcttttg cgtcacgcgc atcaaagtag
     1201 ctgccgccgg cagcgtgcga ccaggtttcg aatttggcgg cgagtttgtc gtcgtcgacc
     1261 gcgaagccga cgatgttgac gcgcacctgc aaccccgact gcacgagctt ctgaatcgtg
     1321 cgcgccgggt tgccgccgca cgtctcctcg ccatcggaga ccaagatcac gacgcgctcg
     1381 cctttggccg cagcgagatc ggcgctcacc ctctcgagtg cggcggagat tggcgtgcgc
     1441 gcgttgccct tcgcttggag cgcagcgatc ttcgaacccg cggcgccgcg atcgagcggt
     1501 gcgaagggaa tgtcgagctc cgattggcag gtgtccgctc cgcgaccgag cacccgaagc
     1561 gcaaacggcg taccgggtgg gatgagctcg ccggtgagct caccgagggt gtgcttcgcg
     1621 atgtcgatgc gccgctccga gccgattcgc tggagcatgc tgccggaggc gtcgagaacg
     1681 acctcgaccg cgccgggagc aaacgccgaa tcggggccgg ctgctttggc ggacagcgtc
     1741 actttgacga accccggctc cgactgtcct ggtatcactc tcaaggtcgc tcgtgcgagc
     1801 acggtgccag tcgcaccggt cttgtagcga agctcgtact cgccggtctc gacgggcgca
     1861 ccgagcccca gcgggctgcc cttcatcgta aaatccccgg cgacggacgt gcggtccggc
     1921 gagccctttt tggcgatcac gatcgcgtcg tactcgcctg ccggtcccgt ccaagtcacg
     1981 ggaaagcgat tgcccgcgac gaccgatggt gcaaccgaaa tcgacgcgct cgcagccgtt
     2041 acggtgaggt tcgcccgggc gagtgttgca ccggcgccag tcagatagcg aatctcgtac
     2101 tcacccggtt gttcgggcac gttgacgccc atcggactgc ttcggccggt aaagttcggt
     2161 gtgcctgccg cagcggtgcg ttcgggtgcg ccggccttca cgatgccaag cttgtcgaat
     2221 tcgttgttcg gtcccctcca agcgacggaa attctcgagc ccgcgacagc ggtagcgggc
     2281 gcctggatcg tggctgtggc ggcgcgcacg gagatctttc tccgcgcgag aaccttgcct
     2341 cccatgaagt agcgaagctc gtattcgccg ggctcctccg gcaacttgac gtacgcgggc
     2401 cgaagctgcg cgtccttgga gccagctggg taacagggga gccccttcgg ggcggtcgtg
     2461 tcgggcgaac cggccggcag agccgcgatc cgatccagca ttccggcggg cccagtccac
     2521 ttcaccgcga ccttggaacc ggccgtcgct gaggccgggg cctcgagact ggcggtagga
     2581 tccgccaccg cccgagcaga atacaagatc cccaacatgg cgagcaaaag acgcaagatt
     2641 gtcccaagtt tcatcgcatc gcctccctga tgttcacgag cccgatacta gacgttcgtc
     2701 ccgcctatgt gatgcagccg cgcgctcact tcactcgtga ccaattggaa cgaatcgtcg
     2761 agcgcgagcc ggagcggttg cgattgaccg atcgacgaaa ctccgacacg acatgacatc
     2821 accgtgaatc gcgccgcgcg tcttccattc ggtttcgttg cctcgctgct ggtcggcctg
     2881 aatctcgctg cgtgcggcaa cgcgaatcca tgccctcgtg tcgtaaccgc ccaaagaact
     2941 ctcccggcgt cggccgtcgc agccgcgagc gccaagccaa gcgccgctca ccccatcgtt
     3001 ccgtgcaccg tcacggtcga caagcccggg tcgctgctcg ccgatttcga gaagcccaat
     3061 gccggcgaag catggttctc ctattccgat ggaacggcgg gcggcgtgct gcgccaagag
     3121 acatcgacct ggaaggatgg tcaacgcgcg cttcatgtca cggcctctgg ctttcgcgag
     3181 tggggcgcgg gattgggcgt tccggtcggg atgctcgcgg ccgcgcaggg tccttgcgag
     3241 ttcgatgcgt ctgcttactc gggcgttcgc tttcgtgcgc gaggtcgcgg cgcactgcgc
     3301 ttggcggtgg ctagcgtccc ttcgatgccg gtcgca
//
LOCUS       DASTKS010000585         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_985683, whole genome shotgun sequence.
ACCESSION   DASTKS010000585 DASTKS010000000
VERSION     DASTKS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985683"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VFQ35_12090"
     CDS             complement(<1..1240)
                     /locus_tag="VFQ35_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931427.1"
                     /translation="MVDAAKLWQRYGTGFISALAGAVVFWVAMRSVGSDWYISQTHAD
                     ILYTAVWRFHEFPFFSFVFGGGNYFIQDPQSNLFSIVNPLVLLTGPSNGLRLAEAVWG
                     GVGCYSFIVWMRRHVSETAAQFGALAWCLSLGVFWRVAVGNDMFLWHLGLPIFLVLIE
                     RLVQERAWKNAVALGLALGVFLLGPTFHTFTYLLVVTLPLFGLLMLITERPGLRAFGR
                     ILALLGGAFILGVVIASPKFACWTRFPMGRPTPDPGDADLFDAVRTLVDYTNTAWFKV
                     PLGHRAGRYQWWGIEEGAVALAPPATLLVPLGVVVGLWSRKRRAHALFAMVLIAIGLT
                     ISSSETVWNFFRELTHGNFRVALRYHALPWFGLTVLATLGADALFNRWPRARAPLAAT
                     FFGGVVAAAVWWVHTAGHLEV"
     gene            complement(1379..3211)
                     /locus_tag="VFQ35_12095"
     CDS             complement(1379..3211)
                     /locus_tag="VFQ35_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931428.1"
                     /translation="MVEAAKFWQRYGPGCISALAGLVVLWVALHSSGSDWYISETHAD
                     ILFTAVRSFHEFPFYSFVFGGGGYFLQDPQNNLFSIANPLVVVAGPTIGLRLAIALWG
                     VIGCYSFIAWMRRHTSELAAQLGGLSLCASLAVFWRVEVGNDMFLWHLGLPILLLLVE
                     KLVAERSWKNAVAFGLALGIFILGPTFHSFTYLYVPVLPIFTLGALAVERPSVRALGR
                     IVALFSFAFALALLIASPKIISWTKFSMGRPVPDPSTGTLVDSLRALLDYRYTSWSKF
                     EMGFFRRGRRLLRHWWGIHEAASALPPPATLLVPVGAVAGLLSKQKRPYALFAFLLVA
                     VGLSLANSDAIWFFLRRITHDSFRVAPRFLVVAWFGLAVLATLGADFLLSRARRAAAL
                     ASGVFVSAVVASALWWTYTAGRVEPRTTTDNILPQVMNPFTIMAEESALASNVKSFDR
                     LVPFQYGEREFLHGNGSIDGFFVVGNGPSPLAPRQRPVAVVRRTGRGASVIAHTTIEL
                     DAIAPEERVTVRIAEPQFGLRVRAYPENAKPQLWWDENGLTVENVGAEPIERLVLRPG
                     LPISRSWFVLSLLAAVGSVGALSYPLWWRKLGAFKRLRRLGVST"
     gene            3456..>4188
                     /locus_tag="VFQ35_12100"
     CDS             3456..>4188
                     /locus_tag="VFQ35_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9931429.1"
                     /translation="MPTALITGVTGQDGSYLAELLLSKGYTVHGTVRRSSSFNRGRID
                     HLAADEKGGRFFLHYGDMMDTSSITRTLERIRPDEIYHLAAQSHVKVSFELPEYTAQV
                     DALGTLRILDVLRETQLNARFYQASTSELYGKVQAIPQSETTPFYPRSPYGVAKLYAY
                     WLTVNYRESYGIFAANGILFNHESPRRGESFVSRKVTLGVARMKHGLQKKLVLGNLDA
                     ERDWGYAPEYVEGMWRILQQDKPEDF"
BASE COUNT          906 a         1379 c         1326 g          577 t
ORIGIN      
        1 gcacctcgag gtggcccgcc gtgtgaaccc accaaacggc cgcggcaacc acgccgccga
       61 agaacgtcgc cgcgagaggc gcgcgcgccc gcggccaacg attgaagagc gcgtcggcgc
      121 cgagcgtcgc caacacggta agcccgaacc agggcagcgc gtggtagcgc agcgcgacgc
      181 ggaagttgcc gtgtgttagc tcgcggaaaa agttccacac ggtctcggaa gacgagatcg
      241 tgagcccaat cgcgatgaga accatcgcaa agagcgcgtg ggcgcggcgc ttcctcgacc
      301 aaagccccac gacgaccccg agcggaacca gcagcgtcgc cggcggtgcg agagccacgg
      361 ccccctcctc gatgccccac cattggtacc ggccagcacg gtgccccaga ggaaccttga
      421 accaggccgt gttggtgtag tcgacgagcg tgcgcaccgc gtcgaacaag tctgcgtcgc
      481 ccgggtcggg cgtcgggcgg cccatcggaa agcgcgtcca gcacgcaaac ttggggcttg
      541 cgatgaccac cccgagaatg aacgctccgc cgagcagcgc gaggatacga ccgaaggcac
      601 gcagcccggg tcgctccgtg atcaacatga ggagcccgaa caggggcagc gtcaccacca
      661 acagataagt aaacgtgtgg aacgtgggcc caagtaggaa cactccgagc gccaggccga
      721 gtgcgaccgc gttcttccac gcgcgttctt ggacgaggcg ctcgatcagc acaagaaaga
      781 tcggcaggcc cagatgccag aggaacatgt cgttcccgac cgcgacccgc caaaacactc
      841 cgaggctcaa gcaccaggcg agcgcgccga actgcgccgc ggtctcgctg acgtggcgcc
      901 gcatccagac gatgaacgag tagcacccga cgccgcccca aaccgcctct gccagccgga
      961 gcccgttcga gggtccggtc aggagcacca gcgggttcac gatagagaag aggttgctct
     1021 gggggtcctg gatgaaatag ttcccgccgc caaaaacgaa cgagaagaac gggaactcgt
     1081 gaaaccgcca gaccgcggtg tagaggatgt ctgcgtgggt ctggctgatg taccagtcgc
     1141 tacccacgct gcgcatggcc acccaaaaca cgacggctcc ggccagcgcc gagatgaacc
     1201 cggtgccgta ccgctgccag agcttcgcag catcaaccat gcgggaaggc ggcctcgtag
     1261 catgggccct cggaggacgt catcctcatt tcagggcgtc tactagcggg atttcacgcg
     1321 ccgcgcccac cttttcaccg tgtatcgggg cggaaacggc tcaatggccg acgacgattc
     1381 aggtcgacac gccgagccgc ctcagtcgct tgaacgcacc aagcttccgc caccacaacg
     1441 ggtagctgag cgccccgact gaaccgaccg ctgccaacag cgaaagcacg aaccaagagc
     1501 gcgagatggg caggcctggg cgcagcacca ggcgctcgat gggctcagcg ccgacattct
     1561 cgacggtgag cccattctcg tcccaccaga gctgcggctt cgcgttctcg gggtacgcgc
     1621 gaacacgcag cccgaactgc ggctcggcga tgcgcaccgt tactcgctcc tccggcgcga
     1681 tggcgtcgag ttcgatcgtc gtgtgggcga tgacgctcgc gccccgcccg gttcgtctga
     1741 cgaccgcaac gggacgttgc cgcggggcga gcgggctcgg tccgttcccg acgacgaaga
     1801 agccgtcgat gctgccgttt ccatgcaaga actcgcgctc gccgtactgg aaagggacca
     1861 gccgatcgaa gctcttcacg ttcgaagcga gagcagactc ctccgccatg atggtgaagg
     1921 gattcatcac ctgaggcaga atgttatcgg tggtcgtacg cggttcgacc cgacccgcgg
     1981 tgtaggtcca ccacaacgcc gaggcgacga cggctgaaac gaacacgccg ctcgccagcg
     2041 cagccgcgcg ccgagcgcga gagagcaaaa agtcggcgcc tagggtcgcg agcaccgcga
     2101 gcccgaacca agccacgacg agaaagcggg gcgcgacgcg gaagctatcg tgcgtgattc
     2161 gccgcaggaa gaaccaaatc gcgtccgagt tggcgaggct cagtccaacc gcgacgagca
     2221 gaaaagcgaa cagggcgtaa ggccgcttct gcttcgagag cagacctgcc acagccccca
     2281 ccggaacgag cagcgtcgcg ggcggaggaa gcgcgctcgc ggcctcatga atgccccacc
     2341 agtgacggag gagacgacgc cccctgcgga agaaccccat ctcgaacttc gaccaggagg
     2401 tgtatcgata atcgaggaga gctcgcagcg aatccacgag tgtgccggtg ctcggatccg
     2461 gaacgggccg gcccatcgag aacttcgtcc aagatatgat tttggggctt gcgatgagca
     2521 gcgcgagcgc gaacgcaaaa gagaagagcg cgacgattcg cccgagcgcg cgaacgctgg
     2581 gacgttcgac cgcgagcgca ccgagcgtga agatcggaag caccggcacg tacaggtacg
     2641 tgaaagaatg aaaagtcggg cccaggatga agatgccgag ggccaaaccg aacgcgaccg
     2701 cattcttcca gctgcgctcg gcgacaagct tctccacgag gaggagcaaa atgggcaacc
     2761 ccaggtgcca gaggaacata tcgttcccga cttcgacccg ccagaacacc gcgaggctcg
     2821 cacacagcga aaggcccccg agctgcgcag cgagctcgct cgtgtggcgc cgcatccacg
     2881 cgatgaacga atagcaaccg atcactcccc aaagcgcgat cgccaagcgc agtccgatgg
     2941 tcggtccggc gacgactacg agcggattcg caatcgagaa aaggttgttc tgcggatctt
     3001 gaaggaagta gccgccgccg ccgaagacga acgagtagaa cggaaattcg tggaacgaac
     3061 gaaccgcggt aaacagaatg tcggcgtggg tctcgctgat gtaccagtca ctgccgctgc
     3121 tgtgcagcgc cacccaaagg actacgagcc cagcgagggc cgagatgcac ccgggaccgt
     3181 accgctgcca gaatttcgca gcctcgacca tacaacaggg cgggctccta ccatgggtct
     3241 agccgggccg tcatccgccg cttccccacc gcatgctcgc cgctcgttcg ctgcgcagac
     3301 gcggccccga gcggctactt ggccggagcg atgctcatgc cgcagcgcgc cggggcgaac
     3361 cacgtggacg tcaggcgcaa tatcgattaa ccgcggtttc actcttgcgc gcggcgggtt
     3421 cgggcgcgag aaagcgacgg gcgaggaaca acgatatgcc gacagcctta attaccggtg
     3481 tcacgggtca ggacggctcg tacctagcgg aactactcct ctcgaagggt tacacggtgc
     3541 acggcaccgt gcgccggagc agctctttca accgcgggcg catcgatcac ctcgccgccg
     3601 acgagaaggg tggacgcttc ttccttcatt acggcgacat gatggacacc tccagcatca
     3661 cgcggacgct ggagcgcatt cgccccgacg agatctacca cctcgcggcc cagagccacg
     3721 tgaaggtgtc gttcgagctc cccgagtaca ccgcgcaagt ggacgcgctc ggcacgctgc
     3781 gcatcctcga cgtgctgcgc gagacgcagc tgaacgcgcg cttttaccaa gcgtcgacga
     3841 gcgagctcta cggcaaagtc caggcgatcc cgcagagcga aacgacgccg ttctacccgc
     3901 gcagccccta cggcgtcgcg aagctctacg cctactggct caccgtcaac taccgagagt
     3961 cttacggcat cttcgccgcc aacggcattc tcttcaatca cgagtcgccg cgacgcggtg
     4021 agagcttcgt gagccgcaaa gtgacgctgg gcgtcgcgcg catgaagcac gggctccaga
     4081 agaagctcgt gctcggtaac ctcgacgccg aacgcgactg gggctacgcg cccgagtacg
     4141 tcgaaggcat gtggcgcatc ctccagcagg acaagccgga ggatttcg
//
LOCUS       DASTKS010000586         6192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_94381,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000586 DASTKS010000000
VERSION     DASTKS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94381"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFQ35_12105"
     CDS             <1..290
                     /locus_tag="VFQ35_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931430.1"
                     /translation="KAAFRPPSRRTRTSLDVALPRHFNRTRAPHLASPELAAVHGPRS
                     TSHGPSFFPLNVERVGEEMLGALALSLGVLALNQAPIARGERVQRMSCRTA"
     gene            complement(263..820)
                     /locus_tag="VFQ35_12110"
     CDS             complement(263..820)
                     /locus_tag="VFQ35_12110"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
                     /protein_id="HET9931431.1"
                     /translation="MPNPRLYGDVDEIENDDRDLSSRSDARRENRELEEKLARVAKQL
                     VEMRHVLLEKLDLPEALLDIVRDAQAMRDPRARNRQLRLVRTALRESNWSLISIRVEA
                     VLKHGALPPALAGDPSDPAAKAPEWAARLIGGGNDAIEAIVLEYPSADRTHLKNLVRE
                     VQKANAERRAKAEARLTQAVRQLMR"
     gene            complement(851..2290)
                     /locus_tag="VFQ35_12115"
     CDS             complement(851..2290)
                     /locus_tag="VFQ35_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9931432.1"
                     /translation="MSSLRQSSYRILSGPLGLEVARFGTPLAVGMGLQTAFNLVDAYI
                     IGELSDDVAGPALGAIGVCDQLAALGTIVSYGFTVATGALLSQKTGQNDQESVRRITV
                     QSLLFVLMLSLVFGGLGLFGAEWFVRDAVGAKGQVADLGVPYLRVMMGGSFSIFLMLH
                     LTSVLRALGSSKTPVTLMLAANVLNFVLAVLLVYGSGPAPLVFSWGPPLARALSLPRM
                     ELMGAAWATLIARVIVLPPLALILLKRLGVLRAHTPWSFDFSILGNIIKMGWPSSAQL
                     VVRIMAMLFTHALVARTFTTASNQTATTALGIVFRLETMALFIGLGWGSAAQTFIGQN
                     LGATNTERAKSSGWYAAGFNAVTMALLALVYWAFGRRLVALFDDDPEVVSMAMDYFRW
                     VGLSYVGLGTGVVLGSAIQGAGATRQALVLDAMVVLLLQLPASLLAVSLRGATPTMLF
                     QAVAITYFAFALVHAYSYWRGRFLRAVIA"
     gene            complement(2287..3246)
                     /locus_tag="VFQ35_12120"
     CDS             complement(2287..3246)
                     /locus_tag="VFQ35_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931433.1"
                     /translation="MSPIHRIGIDENGLGARLGPMVVTAVLAQVDESGARLLSRRLPK
                     AIRKDLDDSKRLVSHADFGLGEAWARAVVADAGHPSALLEALLHEPSEQLRAPCPSHV
                     EAQCWSTGDEAFVASDELCERVAQHLATLAERGVEVLTVRSAVLCTKRLNDSKERGVN
                     RFVADLHAMEHLLLSLRAKAGAKVHAVCGKVGGITEYEKFFGPLSGHLRTALEVGSKK
                     SSYYFPSLGEVSFVYDADARDPIVMLASLIGKYVRELLMARIVRFYRETPDDKAASGY
                     HDPHTNAFVERTALLRRKRNVPAQCFERTRDEGPRDAAAEVTS"
     gene            complement(3364..4215)
                     /locus_tag="VFQ35_12125"
     CDS             complement(3364..4215)
                     /locus_tag="VFQ35_12125"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HET9931434.1"
                     /translation="MNDFTRAPVPRHGDEAMTADREETKYLVPGALVEGIVRAVSRHL
                     DSHRFTGEGANRLPDPHHFVTTIYFDTPSRRHFHAAVGNMTQNVKIRAKEYYDLHPSL
                     AELATDPTQMVKGQPWVWFEIKRRNGSQTTKRRFRLAKKDMPRVLQAQRLSAEALLET
                     ERDTAVAGLSELADYVQSLSEPLTANTLVHYRRLSWQNPDSTLRVTLDLGLSFHTPPT
                     DLWTRDQALLRSAVGSLRHPFGSAVLEVKRRASLPAWLTEALEHARLEPQSFSKFVAG
                     SRSIHGA"
     gene            complement(4212..5009)
                     /locus_tag="VFQ35_12130"
     CDS             complement(4212..5009)
                     /locus_tag="VFQ35_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931435.1"
                     /translation="MNSKKAFFGAAALALVSLGALSGCGSDAPVNSATGGSGANGGSG
                     ANGGNSANGGTGNVGAATEPLLPWAVGNTWTYRVTENGMVSTKTTTVMELTTVGGVGA
                     LKDTPAYLVVTKKGANGNDKTESYQAPDPAEPDRILRYRELSYGAMTGALQLEEYWVP
                     ARIHADGSKVGTANWVDSYTEYKLPVGMQAPPGAETHDLWIVVSADEAIDSGGKHYDH
                     AVHLQKTGGSTKDYWYVRGVGKVRETGGQTEELLDAKLGTSINSLKK"
     gene            complement(5041..>6192)
                     /locus_tag="VFQ35_12135"
     CDS             complement(5041..>6192)
                     /locus_tag="VFQ35_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9931436.1"
                     /translation="KLTVGASSWDAVEASQKEADDWAAAHAPPPIKLPTLNLSVTDLR
                     DVKLMVDEKGPGTLRDQISASIQAFGTKASNYGVLANTMNVPIRPAFGIPAYYYDQFM
                     RENHLYERVDALLADATFIADASVRDQKLAALRDDMINSSSIVINADFQAALKTKLEK
                     DFPNQTMRFRTSTNAEDLDGFPCAGCYDSFTGDPTNWEGSLLHAIRKAWAGIWYFRTF
                     EERRFHSIDHKTVAMSLLVHHNFPAEEANGVASTANPYDPTGSEPGFYINVQFGGDAE
                     VVHPPPGVTSDQLIYFYDQSTVTPSYLAHSNITTPQGAHVLTDAQLQELGAALSAIHS
                     RFRPAYGPGVGFSDWYAMDVEFKFDDEGTTDGKPHLWVKQARPYPGRGQ"
BASE COUNT         1029 a         1989 c         2050 g         1124 t
ORIGIN      
        1 ccaaggcggc gtttcgcccg ccctcgcgcc gtacacgtac ctcgctcgac gtcgcgctgc
       61 cacgtcactt caatcgaact cgcgccccgc acctcgcctc acccgagctc gccgccgtac
      121 acggacctcg ctcgacgtcg cacggccctt cgttctttcc gctcaatgtc gagcgagtgg
      181 gggaagaaat gcttggtgcg ctcgctctgt cccttggcgt cttggcgttg aatcaagcgc
      241 ccatcgcgcg gggggagcgg gttcagcgca tgagttgccg gacggcctga gtgaggcgcg
      301 cttccgcctt cgcgcgccgt tcggcgttcg ccttttggac ttcgcgcacg aggttcttca
      361 ggtgagtgcg gtcggcgctc ggatattcga ggacgatggc ttcgatcgcg tcgttgccgc
      421 cgccgatcaa acgcgctgcc cactcgggcg ctttggcggc ggggtccgag ggatcgcccg
      481 cgagcgccgg gggaagcgca ccgtgcttca gcacggcctc cacgcggatg gagatcagcg
      541 accagttgct ctcgcgcagc gcggtgcgca ctaggcgcag ctgtcggttt cgggcccgcg
      601 ggtcacgcat ggcctgtgca tcgcgcacga tgtcgagcag cgcctcgggg aggtcgagct
      661 tttcgagcag cacgtgtcgc atctcgacga gctgcttggc gacgcgcgcg agcttttcct
      721 cgagctcgcg gttctctcgg cgcgcatcgc tgcgcgacga cagatcgcga tcgtcgtttt
      781 cgatctcgtc tacgtcgccg tacaaccgcg gattcggcat ggcggccgga atcttacacg
      841 ccgagagcgc tcacgctatg accgcgcgca aaaagcggcc ccgccagtag ctgtaggcgt
      901 ggaccaacgc gaaggcgaaa taggtgatgg cgaccgcttg aaagagcatg gtcggcgttg
      961 cgccgcgcag agaaacggcg agtaaactcg cgggaagctg cagtaacaac acgaccatcg
     1021 cgtccaggac cagcgcctga cgtgtcgcgc ccgcaccttg aatcgcggag ccgaggacga
     1081 cgccggtacc gaggcccacg tagctgagcc cgacccagcg gaagtagtcc atggccatgg
     1141 aaacgacctc cgggtcgtcg tcgaagagcg ctaccaagcg cctgccgaaa gcccagtaca
     1201 cgagcgcgag cagggccatc gtgactgcgt tgaagccggc ggcgtaccag ccgctgctct
     1261 tcgcgcgctc cgtattcgtc gcgccgagat tttgaccgat gaaggtctgc gcggcgctgc
     1321 cccaaccgag gccaatgaag agcgccatcg tctcgagccg gaacacgatg ccgagcgccg
     1381 tggtcgcggt ttggttgctc gcggtcgtga aggtgcgcgc gacgagggcg tgcgtgaaca
     1441 gcatcgccat gatgcgcacg acgagctgcg cgctcgaagg ccagcccatc ttgatgatgt
     1501 tgccgaggat cgaaaagtcg aagctccacg gcgtgtgggc gcggaggacg ccgaggcgct
     1561 tcagcaaaat caaggcgagc ggcggcagca cgatcacccg cgcgatgagc gtcgcccaag
     1621 cggcgcccat gagctccatg cgcggtaacg acagtgcgcg cgcgagcgga gggccccacg
     1681 agaacacgag aggcgccggc ccggagccgt acaccagcag caccgcgagt acgaagttca
     1741 gcacgtttgc cgcgagcatc agggtgaccg gcgttttgct gctgccgaga gcgcgcagca
     1801 cgctcgtcaa gtgcagcatc aagaagatgc tgaagctgcc gcccatcatc acgcgcaggt
     1861 atggcacgcc gagatcggca acttgcccct tcgcgcctac ggcgtcgcgc acgaaccact
     1921 ccgcgccgaa cagcccgagc cccccaaaaa ccagggacag catcagcacg aacaagagcg
     1981 actgcaccgt gatacggcgc acgctctcct ggtcgttttg tcccgtcttt tggctcaaca
     2041 gcgcgccggt cgcgacggtg aagccgtaac tcacgatcgt gccgagcgcc gcgagctgat
     2101 cgcagacgcc gatcgcgccg agcgccggcc cggcgacgtc gtccgacaac tcgccgatga
     2161 tgtaagcgtc gaccaagttg aaggcggttt gcagccccat cccgaccgcc agtggtgttc
     2221 cgaatcgagc gacttcgagc ccgagcggcc ccgacaaaat ccgatagctc gattggcgaa
     2281 ggctactcac gaggtcacct cggccgcggc gtcgcgcggg ccctcgtcac gggtccgctc
     2341 gaagcattga gccgggacgt tgcgcttgcg cctgaggagg gcggtgcgtt cgacgaaggc
     2401 gttcgtgtgc gggtcgtggt agccgctcgc agctttgtcg tccggcgttt cgcggtaaaa
     2461 gcgcacgatg cgcgccatca agagctcgcg gacgtacttc ccgatcagag aggcgagcat
     2521 cacgatcgga tcgcgcgcgt cggcgtcgta cacgaagctg acctcgccga gcgaggggaa
     2581 gtagtagctg ctctttttcg agcccacctc gagcgcggtt cgcaggtgac cgctcagcgg
     2641 accgaagaac ttctcgtact cggtgatgcc accgactttg ccgcacactg cgtgcacttt
     2701 ggcgcctgcc ttggctcgca acgaaagcaa aaggtgctcc atcgcgtgta ggtcggcgac
     2761 gaagcggttc accccccgct ccttggagtc gttcaggcgc ttcgtgcaga gcacggccga
     2821 gcgcacggtg aggacctcga cccctcgttc ggccaaggtg gcgaggtgct gcgcgacgcg
     2881 ctcgcagagc tcgtccgaag ccacgaaggc ctcgtcaccg gtgctccaac actgagcctc
     2941 tacgtgagag gggcagggtg cacggagctg ctcgctcggc tcgtgaagca atgcctccaa
     3001 gagtgcgctc gggtgcccag cgtccgcgac gacggcgcgc gcccacgcct caccgaggcc
     3061 gaagtccgcg tgcgacacga gccgcttcga gtcatcgagg tccttccgaa tagccttcgg
     3121 caggcgccga gaaaggagcc gcgcgccgct ttcgtcgacc tgcgcgagaa ctgccgtgac
     3181 aaccatcggg ccgaggcgag cgccgaggcc attctcgtcg atgccaatgc gatggatggg
     3241 ggacacgcgc cggccggcag catacagcgg cgcgcagctg ccggcccagc gctaatcgcc
     3301 agcagcgcgc tgtgaaaaaa cgttcgcgct tgcccgagcg cgctctgcgg aaccggcgtg
     3361 cggctaggcg ccgtggatcg agcgggagcc tgcaacgaat ttgctgaagc tctggggttc
     3421 tagccgggcg tgctcgagcg cctcagtgag ccaggcgggc agagacgcgc ggcgcttgac
     3481 ctcgaggacc gccgagccga atggatggcg caagctgccg accgcgctcc gcagcaaggc
     3541 ttgatcgcgc gtccagaggt cggttggtgg cgtgtgaaag ctgagcccca ggtcgagcgt
     3601 gacccgcagc gtgctgtccg gattttgcca ggacagcctg cggtaatgca ccagagtatt
     3661 cgcggtgagc ggctcactga gcgactgcac gtaatcggcg agctcgctga gccccgcgac
     3721 ggcggtgtcc cgttccgtct cgaggagcgc ctccgccgag agccgttgtg cctgcagcac
     3781 gcgcggcatg tctttcttcg cgaggcggaa gcgacgcttc gtcgtctgcg agccgttgcg
     3841 tcgtttgatc tcgaaccaaa cccacggctg gcctttcacc atctgcgtgg gatcggtcgc
     3901 gagctccgcg agcgaggggt gcagatcgta gtactccttc gcgcggatct tcacgttttg
     3961 cgtcatattg ccgacggcag cgtggaagtg ccggcgcgaa ggcgtatcga agtagatggt
     4021 cgtgacgaag tgatgcgggt ccggcaagcg gttcgcgcct tcaccggtga accggtgaga
     4081 atccaggtgg cgcgataccg ctcgcacgat accttcgacg agcgcgcccg gaacgaggta
     4141 cttcgtctcc tcgcggtcgg cggtcatggc ctcgtcgccg tgacgaggca ctggggcgcg
     4201 cgtaaagtcg ttcacttctt cagcgagttg atggaggttc cgagcttcgc gtcgagcagc
     4261 tcttcggttt ggccgcccgt ctctcgcacc ttgccgacgc cgcgcacgta ccagtagtct
     4321 ttcgtgctcc cgcccgtctt ctgcagatgc accgcgtgat cgtagtgctt accgccggag
     4381 tcgatggcct catcggcgga gacgacgatc cagagatcgt gggtttccgc gccggggggc
     4441 gcctgcatcc cgacgggcag cttgtactcg gtatacgaat ccacccagtt cgcggtgccg
     4501 accttgctgc cgtccgcgtg aatgcgcgcc ggcacccagt actcttctag ttgaagcgcg
     4561 cccgtcatcg ccccgtacga cagctctcgg tagcggagga tgcgatcggg ctcggcggga
     4621 tccggcgctt ggtagctctc ggtcttgtcg ttcccgttcg cgcccttttt cgtcacgacg
     4681 aggtaggccg gcgtgtcctt cagcgcgccg actccaccga cggtcgtaag ctccatgacg
     4741 gtggtggtct tggtactcac cattccgttc tcggtgacgc ggtaggtcca ggtgtttccg
     4801 accgcccagg gcaggagagg ttcggtcgcg gcgccgacgt tgccggtgcc accgttcgca
     4861 ctgttgccgc cgtttgcacc gctgccgccg tttgcaccgc tgccgcccgt tgcgctgttc
     4921 acgggcgcgt ccgagccaca accgctgagc gctccgaggg acaccagagc gagcgctgcg
     4981 gctccaaaga atgctttctt gctattcacg tttcgcatca ctttccaaat gcctcgcggt
     5041 tcactgcccg cggccggggt agggccgagc ttgcttcacc cacaggtgag gtttgccgtc
     5101 ggtcgttcct tcatcgtcga acttgaactc tacatccatg gcgtaccaat cgctaaaccc
     5161 caccccgggg ccataggccg gccggaaacg tgagtgaatt gcggagagtg ccgcaccgag
     5221 ctcttgaagc tgcgcgtcgg tgagcacgtg agcgccctga ggcgtcgtga tgttggagtg
     5281 ggcaaggtag ctcggcgtaa ccgtcgactg gtcgtagaaa tagatgagct ggtcgctcgt
     5341 gacgccaggc ggcgggtgta cgacctcggc gtccccaccg aactgtacgt tgatgtagaa
     5401 ccccggctcg ctcccggtcg ggtcgtaagg gttagccgtc gacgcgaccc cgttcgcctc
     5461 ctccgctggg aagttgtggt ggacgagcag cgacatcgcg accgtcttgt gatcgatcga
     5521 gtggaacctg cgctcctcga aggtgcggaa gtaccaaatc cccgcccacg cttttcggat
     5581 cgcgtggagc aagctgcctt cccaattggt tgggtcgccg gtgaacgagt cgtagcagcc
     5641 ggcgcagggg aagccgtcga gatcctccgc gttcgtgctg gtacggaagc gcatcgtttg
     5701 gttcgggaag tctttttcga gcttcgtttt caaggccgct tggaagtcgg cattgatgac
     5761 gatgctcgag ctgttgatca tgtcgtcgcg gagcgcggcg agcttctggt ctcgcacgct
     5821 cgcgtcggcg atgaacgtcg cgtcggcgag caaggcatcc acgcgctcgt acaagtgatt
     5881 ctcgcgcatg aactggtcgt agtagtacgc gggaattccg aaggcgggcc gaatcggcac
     5941 gttcatcgtg tttgcgagta cgccgtagtt gctcgccttc gttccgaacg cctgaatcga
     6001 cgcgctgatc tgatcgcgca aggtgccggg gcccttctcg tcgaccatga gcttcacgtc
     6061 gcggagatcg gtgacgctga ggttcaacgt cggcagcttg atcgggggcg gggcgtgcgc
     6121 tgcggcccag tcgtccgctt ctttttggct tgcctcgacc gcgtcccagc tgctggcgcc
     6181 gacggtgagc tt
//
LOCUS       DASTKS010000587         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_94525,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000587 DASTKS010000000
VERSION     DASTKS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94525"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VFQ35_12140"
     CDS             <1..1406
                     /locus_tag="VFQ35_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931437.1"
                     /translation="VQEDADDVLARVTCAWALVRSLRRSGDSALAAEAEALLATTTLA
                     DGPIATLLTALRAALAAERGQHAEALTLAERALSQDARQVDALATVAVASAGLGKAAE
                     AERTQRRLRELAPDEAVANDAAILRRRHGRDAPKAPPVPPARWGAEEAALTRGDSLPA
                     LELLARKARDALRAQQRRGGTEVWHALAMQAAHLLTELPVFRHFAPYDCSVFSVDRLE
                     AALSLLFGSGSPRTVLDEATVVLLGAYVGESWRQAFGADWHGVLSFPFAASVQGIGLM
                     VRPCERVRERLEQGVALGIETPHSLHPGADPLGNSVPLSLVPPAPWDPAEFPSVAEFS
                     ALGRNLGESVIGLYCERTLGLRLDLSISTAVAIDRYVALLAPSKAPPDPNARWARRVA
                     LLLGAYLGEILVDAVAASWENIVAPKSLEDYRLVLPHGNAATLAVRVEERLTGRRAGA
                     LAEHVARLSSGRNSISV"
     gene            1621..2829
                     /locus_tag="VFQ35_12145"
     CDS             1621..2829
                     /locus_tag="VFQ35_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9931438.1"
                     /translation="MFSSRRPGSSIAFVFLLAACSSPSESSQGTGGANSGGSANNGGS
                     ADAQGGAPPSGGAPANGGTNPSGGIASGGTDANGGAKSSGGASNGGAKSSGGAATGGG
                     ASSSGGAKSSGGASTTGGTAGNASGGSSSAGAPAGAAELCTRWKSDRMNMKEDAWNGN
                     AASCDAGDMTAASRDTVLRLVNLYRAIAQLPAVTFDPTRNQQDQACALMMQANGTIDH
                     EPPDTWKCYSADGAKAAGNSNLSSTSAVAGVDGYMLDPGNATTLGHRRWILSNSLGPI
                     GVGSTGKYSCMWTLGGTGKAGKPWMAWPSAGVFPLEAFGGPRQTIDSTGWSLQSDSIN
                     LSGAKVAVTFNGVSQPVTVTQLDPNYGSRYAIRFNPMGWTSAVGAYHVSVTGIPTAIE
                     YDVNVVACTP"
     gene            3073..3402
                     /locus_tag="VFQ35_12150"
     CDS             3073..3402
                     /locus_tag="VFQ35_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931439.1"
                     /translation="MRERDVSVGVKPALAAYDASDCLRFEKTQQPYSIDLEAKSACES
                     KLECSLDYSVRCESREGKTTSRTAHGVRFSIDAGRSAELSLSAEACKQSWTIDDVRWS
                     CHTVPSR"
     gene            complement(3471..>3813)
                     /locus_tag="VFQ35_12155"
     CDS             complement(3471..>3813)
                     /locus_tag="VFQ35_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9931440.1"
                     /translation="GLGLELAFPRKGGSMPEPVEFRINPGSHLMLVPPDGFAFVLGGG
                     RALDVAEARAHECVIGFPFESETAIAETYARAIAHGATAIAPPGPQPWGYSASFRDLD
                     GHVLLLSLRKL"
BASE COUNT          660 a         1265 c         1281 g          607 t
ORIGIN      
        1 ccgtgcagga agatgccgac gacgttctgg cgcgagtcac gtgcgcctgg gcgctcgtgc
       61 gctcgctgcg acgaagcggc gactccgcgc tcgcggcgga agccgaagcc ctcctcgcga
      121 ccacgactct cgccgatggg ccaattgcaa cgcttttgac ggcactgcgc gcagcgctcg
      181 cggcggagcg cggacagcac gccgaagcgc tcaccctagc cgagcgcgcg ctgtcgcaag
      241 acgcgcgaca ggtggacgcg ctcgcgaccg tagccgttgc ttcggcaggg ctcggaaaag
      301 ccgccgaggc cgagcgaacg cagcgccgcc tgcgcgaact cgcacctgat gaagccgtcg
      361 cgaacgatgc tgcaatcttg cgtcgacggc acggacgcga tgcgccgaaa gcgccgccag
      421 tcccgcccgc gcgatggggc gcagaagagg ccgcgctcac gcgaggtgac tcgctccctg
      481 cgctcgagct tctggcgcgc aaagccagag acgctctgcg cgcacagcaa cgacgcggcg
      541 ggaccgaggt ctggcatgcg ctcgcgatgc aagccgccca ccttctcacg gagctgccgg
      601 tgtttcgtca cttcgctccg tacgactgct cggtgttcag cgtggatcgc ttggaagctg
      661 cattgtcgct cctattcggc agtggcagcc cgcgaaccgt cctcgacgaa gccacggtcg
      721 tgttgctcgg cgcgtacgtc ggtgagagct ggcgccaggc cttcggcgcc gactggcacg
      781 gcgtgctttc gtttccgttc gcagccagcg tgcagggcat cggcctgatg gtgcggccgt
      841 gcgaacgcgt gcgggagcgg ctcgagcaag gtgtcgcact cggcatcgag acgccgcatt
      901 cgttacatcc cggcgccgat ccgctcggca actcggtgcc actttcgctc gtgccccccg
      961 ctccgtggga tccggctgag tttcccagcg tcgccgagtt cagcgcgctc ggtcgaaacc
     1021 tgggcgagtc ggtgattggc ctctactgcg agcgcaccct cggcttgcgg ctcgatttgt
     1081 cgatctcgac tgcggttgcc atcgaccgct acgtggcgtt gctcgcgccg tccaaggcac
     1141 cccccgaccc caacgctcgc tgggcgcgcc gtgtcgcgct gctgctcggc gcatacctcg
     1201 gagagatcct cgtcgacgcc gtcgcggcga gctgggaaaa tatcgtggcc cccaagtctc
     1261 tcgaggacta ccgcttggtc cttccgcacg gcaacgcggc cactctcgcg gtgcgcgtcg
     1321 aggagcgcct gaccggccgc cgtgcgggcg ctcttgccga gcacgtcgcg cgcctttcga
     1381 gcggaagaaa ctcgatcagc gtatgagacc accactggct gggacgacaa tggtgcgcac
     1441 agaagaaaag tgagctccgg cgcgaagcgc tcgcaacgct tttcgcggcg ccgtggtggt
     1501 gagccccgag gcgacacccg gcgccgtccc caaaagaggc gaagatttct gacctctcgc
     1561 gacgagttgg cgtcacaatt tgggcaagcg cgcgagcgtg atagcgcgca tcgaggttcg
     1621 atgttttcat cccgtcgccc cggtagctcg atcgcgttcg tgtttctgct cgccgcctgc
     1681 tcgagcccga gcgaatcgtc ccaaggaact ggtggcgcga actccggggg tagtgcgaac
     1741 aacggcggta gcgccgacgc acagggaggc gcgccgccga gcggtggggc tccggccaac
     1801 ggcggaacga acccgagcgg aggtatcgca agcggcggga cggacgcgaa cggaggggcg
     1861 aagtcgagcg gcggcgcttc gaacggcggc gcgaaatcga gcggcggcgc cgctaccggt
     1921 ggtggcgcgt cgtcgagcgg aggagccaag tcgagcggtg gcgcttctac gacgggcggc
     1981 accgcgggca acgcgagtgg cggcagctcg agtgcgggcg cacccgcagg cgcagccgag
     2041 ctctgtacgc gctggaagag cgatcgcatg aacatgaagg aagacgcctg gaacggcaac
     2101 gcggcgagct gcgatgcggg cgacatgacc gccgcatctc gcgacacggt gctgcgcctc
     2161 gtgaacctgt atcgagccat cgcccagctg cccgcggtca cgttcgaccc gacgcgcaac
     2221 cagcaggacc aagcctgtgc gttgatgatg caggccaacg gcacgatcga ccacgagccg
     2281 cccgacacct ggaagtgcta cagcgccgac ggcgcgaagg ctgctggcaa tagcaacctc
     2341 tcgagtacga gtgccgtcgc gggtgtcgac ggttacatgc tcgaccccgg caacgcgacg
     2401 actctcgggc accgacggtg gatcctgtcg aattcgctcg ggccgattgg cgtgggctcc
     2461 actggcaaat attcgtgcat gtggaccttg ggtggcacgg gcaaagccgg aaagccctgg
     2521 atggcatggc cgtccgcggg cgtgttcccg ctcgaggcgt tcggagggcc tcgccagacc
     2581 atcgactcga cgggctggag ccttcaaagc gacagcatca acctgagcgg tgccaaagtc
     2641 gccgtgacgt tcaacggggt aagccagccc gtcacggtga cccaactcga cccgaactat
     2701 ggctcccgct acgccatccg gttcaatccc atgggctgga cttcggcggt cggggcgtac
     2761 cacgtcagcg tgaccggcat cccgacggcc atcgagtacg acgtgaacgt ggtggcgtgc
     2821 acgccgtagc atccaacccc aagctccccc tcccggattt cgaatcggct ccacggcgct
     2881 gagcttgccg gaactttttg gggaacctag gcgggccgcc gctgtcgatc ggggcgttgg
     2941 cggcgcgttc gggcacggaa ccccgagcgg ctagcgctgg tttctcgagt tccggggaga
     3001 aagtctaccg tggatcgtca cgtgctcgcg gcgctagccg cgtcaggagc cctttgtttg
     3061 ctttcagtgc aagtgaggga gcgcgatgtc agcgtcggag tcaagcccgc gctcgccgcg
     3121 tacgatgcga gcgactgcct gcgcttcgaa aaaactcagc aaccctactc catcgacctc
     3181 gaggcgaaga gcgcgtgcga atcgaagcta gagtgttcac tcgactacag cgtgcgctgt
     3241 gagagccgcg aggggaaaac gacgtcgcgg actgcacacg gcgtgcgctt cagtatcgac
     3301 gcaggtcgaa gcgccgaact ttcgctctcc gccgaggctt gcaagcagtc ctggaccatc
     3361 gacgatgtgc gctggagttg tcacaccgtc ccctcgcggt agcacgagcg caatcatcgt
     3421 ccgcgatgta cgccgtgcct cgcattggtt actcattcat tcgcgggccc tcagagcttg
     3481 cgcaagctca gcaacaggac gtgcccgtcg agatcgcgaa aactcgcgga gtagccccag
     3541 ggctgtggac ctggcggcgc gatggcagtg gccccgtgtg cgatcgctcg cgcataggtc
     3601 tccgcgatcg cggtctcgct ctcgaacgga aaaccgatca cgcactcgtg agcgcgagcc
     3661 tcggccacgt cgagcgcgcg tcccccgccg agaacgaacg caaacccgtc gggcggtacc
     3721 aacatcaagt gtgagccggg gttgatccga aactcgactg gctctggcat ggagccacct
     3781 ttacgcggga aagccagctc caaaccgagc cca
//
LOCUS       DASTKS010000588         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1655716, whole genome shotgun sequence.
ACCESSION   DASTKS010000588 DASTKS010000000
VERSION     DASTKS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655716"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..663)
                     /locus_tag="VFQ35_12160"
     CDS             complement(292..663)
                     /locus_tag="VFQ35_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931441.1"
                     /translation="MLLLAVVSSVLALECSTIVGNELDDVRCTADNAVGPPACESGWF
                     CGYGRCRVCAATEACGDDLDNDCNGRRDDGCAGAGGLGSGGADGGSGGTGLASGGGGA
                     SANSGSGGGSTSAGTGGRAAQ"
     gene            complement(681..1481)
                     /locus_tag="VFQ35_12165"
     CDS             complement(681..1481)
                     /locus_tag="VFQ35_12165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931442.1"
                     /translation="MSPPKAQFALCRAPAWLGLGVLWAALSMTGTSRADDSNPNDEEP
                     RAASGGASAKHHFDEALAHYREGHYRAAIAELKAALTFDPTSKDLIYNLALVHEKLGE
                     LDQAIAALERYAELETDPKELARARQAKTRMQGARAELSAPVVVPSRVPIPPVAVPIN
                     ERRTNPWLVASTVVAATGGVLGVVFGVRALVLAPGESASTNASTNIDDLHDQQNRAAF
                     SARVADVSFGVGVVSGIFAAILWTREQPKLAPTTTGPSSLGLRWRTTF"
     gene            1615..2454
                     /locus_tag="VFQ35_12170"
     CDS             1615..2454
                     /locus_tag="VFQ35_12170"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET9931443.1"
                     /translation="MDAGSPQKLNTTALLAAISGVIGFCFWGIGGVLAIVLGITARTE
                     IARSAGRESGSGAAVAGIALGALNIASCVIAMAVGIAYLARPTPAPTPMPAAPIVAVP
                     TTTAPSPRTPKSSPSENATRERTPAEARFGTVRLVDVSTSSEKSLRRVLEREQRAASA
                     ANERLVVFVVGPNCLPCNGVMMSLGDPRMQRALPRVRLLRVDASEFGADLGALGVPAD
                     TVPGFALLGAGQRVTDYVDGGEWDADVAQNIAPVLGAFIEGKYRVRRRQYQSEERPDQ
                     TAL"
BASE COUNT          485 a          962 c          916 g          459 t
ORIGIN      
        1 agctcatggc gatccacgaa cgcgagcccg tgctctgggc acttcgcgac cgtgcccgag
       61 ggcggcggca tgtcctggaa cgcagcgaca ggctggcgac agctcggaca gacgcgctcg
      121 atttcgtcgt gatctgaggc caaggccgtc atcgggattc gcgtcaggcc tcgggagttt
      181 tagccgacgt cctcgtgagc gccagcctga aaatttggcg tcaagggctg gtacgatatt
      241 gcgcgcgagt cgcgtacaca ggcgccggcg ggtacgactt agcccacccc actattgggc
      301 ggctcttccg ccggttcctg cactggtcga accacctccg ctgccgctat tcgccgaggc
      361 gccccctcct ccgctagcca aaccagtgcc acccgagcca ccgtcggctc caccgctccc
      421 cagaccgccc gcccccgcgc agccgtcgtc acgccgcccg ttgcagtcgt tgtcgaggtc
      481 gtcgccgcac gcttccgtag cggcgcacac gcggcatcgg ccgtatccgc agaaccagcc
      541 cgactcgcac gccggcggac cgactgcgtt gtcggccgta cagcggacat cgtcgagctc
      601 gttgccgacg atcgtcgagc actcgagggc gagcaccgaa gagacgaccg caaggagcag
      661 cacggctcga ccgactctct tcaaaaggtc gtcctccagc ggagccccaa cgaggacggg
      721 cccgtcgtcg tcggggcgag cttcggctgc tctcgcgtcc agagaatcgc cgcgaagatt
      781 ccggagacta ccccgacacc gaacgatacg tccgcgaccc gcgccgaaaa cgcggcccgg
      841 ttttgttggt cgtgcaggtc gtcgatgttg gtcgaggcat tggtcgacgc ggattcgccg
      901 ggggcgagca cgagggcgcg cacgccgaac accactccca gcacaccccc ggtcgcggcc
      961 acgacggtgc tcgcaacgag ccaaggattc gtgcgtcgct cgttgatggg cacggcaacg
     1021 ggcgggatcg gcacgcgcga tggcacgacc acgggcgcgg aaagctcggc gcgcgcaccc
     1081 tgcatgcgcg tcttcgcctg ccgagcgcgc gcgagctctt tcggatccgt ctcgagctcc
     1141 gcatagcgct ccagcgcggc gatggcttga tcgagctcgc cgagcttctc gtgaaccagc
     1201 gcgagattat agatgaggtc tttgctcgtc ggatcgaaag tgagcgcagc cttgagctcc
     1261 gctatcgcgg cccggtagtg cccttcgcga tagtgagcca atgcttcgtc gaaatggtgc
     1321 ttggccgacg cgcctcccga ggcggctcgc ggctcctcgt cattcgggtt ggagtcgtcg
     1381 gcgcgcgacg tgcccgtcat gctcagcgcg gcccagagca ccccgagccc tagccaagcc
     1441 ggcgctctgc agagcgcgaa ctgagctttg ggaggggaca tggcgccggg cagcatacac
     1501 tagagcgaca tacggttagg aaatcgccga aggtagcgga aagtggctcc gcacccagta
     1561 gcggcttggg gagagtctct gcgcaccgga ggcgggcgat ctatactggc tgcggtggac
     1621 gctgggtctc ctcagaagct caacaccacg gcgctcctcg ccgcgataag cggagtcatc
     1681 ggcttctgct tctggggaat tggcggagtg ctcgcgatcg tacttggaat cactgcgcgc
     1741 accgagatcg ctcgttcggc cggacgggaa agcgggagcg gcgccgcggt cgcgggcatc
     1801 gcgctcggag ccctcaacat tgcgagctgt gtcatcgcga tggccgtggg aatcgcctac
     1861 ctcgcgcgtc cgacgccggc cccgactccg atgcctgccg caccgattgt cgcggtgccc
     1921 accaccactg ctccgtcccc gcgaacgccc aagtcgagcc cctcggagaa tgccacccgc
     1981 gagcgaactc ccgctgaggc tcggttcggc acggtgcgcc tcgtcgacgt cagcacgagc
     2041 tccgaaaagt ccctgcgccg cgtactcgag cgcgaacagc gcgctgcttc ggccgccaat
     2101 gagcgcctgg tcgtgttcgt cgtcgggccc aactgtctac cgtgcaatgg cgtcatgatg
     2161 agcctcggag acccgcgcat gcagcgcgct cttccgcggg tgcgattgct acgtgtagac
     2221 gcgagtgagt tcggagccga cctcggcgcg ttgggcgtgc cggccgacac cgtgccgggc
     2281 ttcgcgctct taggggccgg gcagcgagtc accgactacg tagatggtgg ggagtgggac
     2341 gcggacgtcg cacaaaacat cgcgccggtc ctcggtgctt tcatcgaagg caaataccga
     2401 gtgcggcgcc ggcaatacca gagcgaggag cgcccggatc agacggccct ttaaaagcgg
     2461 ggcgccgcga ccgtagacgg aacagcagac gctcttcgcc gctgggcgga agcccctctc
     2521 gcccgcattc gtaaacctgc ttaagaaacc ggcaaacctc gccgagctgc ctacaaggtt
     2581 gaacgtcggt tgaactttgt tgacccgatc ttcgagtttc ggtgcgatgg cgcagcgcac
     2641 acgtcgaaag ttgtgccacg gcgcgcacgg caagcgccgt aacgggcaga ggaccgacgg
     2701 gaccccttca ccgcagcaac ctgaactttg ctagtatccg ccccccggat gacgagctcg
     2761 caacagcacc taatcacgcc catccccgtt ggaactgggg tcgttccagg caccttgcgc
     2821 gc
//
LOCUS       DASTKS010000589         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_986105, whole genome shotgun sequence.
ACCESSION   DASTKS010000589 DASTKS010000000
VERSION     DASTKS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986105"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2326)
                     /locus_tag="VFQ35_12175"
     CDS             complement(<1..>2326)
                     /locus_tag="VFQ35_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET9931444.1"
                     /translation="RSDYRPGDIIDADLSVSGTDGRRGRSSVTFYAVDEGVLMLTGYQ
                     TPDPLPSFTTERKLAVFGLENRESLAKILAMKNGERVRTLGYDFLNPAGNDKGYDGGG
                     GGEDAVRADFKTTAFFEAGRVTSAEGTTHFRFKLPDNLTTFRLMAIASAEDERFGFGE
                     ARITTSRKLMARPALPRIVREGDEFEAGVVVSSKDLPDSNVSVTLRATGLVASGPAAR
                     QVTVPRGRSVEVRFPVKAQKSGPVSLEFGVNGANQRDSVLVKRQVELPVSREAVAVYG
                     ETSRAAAVELGNLGDVRPESGGLDVHLASTALAGIGEAFERNLEYPYGCTEQLVSRTL
                     PLLLLSDLARAFHVRMPAKVDDVVDDALGEILSHQTNSGGFGYWDGDWADVPWLSAYA
                     VLALETAADKRFYVPKSARDQAIAYLQRVVSSARFTNNAPDDDADVEPRDLLDEERTR
                     AGSYADVAFVADVLATVGQAQPGTLNQLFDARRDKPLFTQALLLHAMARAKMPRATQN
                     ILATELTNHLRVGANEAFVDDQSDDYAPWLDSSIRTTALVLRAFLAMDPHHPLAARLA
                     RGLLSRREHGAFRSTQENTWALLALDDYRKNQETAEPNFDAQVFLGDDLLGNASFHGS
                     SAIEVPFQIPASKLGQKRGPLTFAVNGPGKLFYAAQLRYASAKLPTRPDDTGFSVQRL
                     VRALKPSELAEAQKTVPTSGQSKASVNDLVMMDVLFETAEPREQVVLDDPLPAGLEPV
                     DFALQTSSMHAQVSDGPTTANQPRGAGTLGYGAFR"
BASE COUNT          375 a          735 c          781 g          435 t
ORIGIN      
        1 cggaacgcgc catacccgag cgtccccgct ccccgaggtt ggttggcggt cgtcggaccg
       61 tcgctcacct gcgcgtgcat cgacgacgtt tggagtgcga aatcgaccgg ctcgagcccg
      121 gccggaagcg gatcgtcgag cacgacttgc tcgcgaggct ctgccgtttc gaacagcacg
      181 tccatcatca cgaggtcgtt tacggaagct ttcgactgcc cgctcgtggg tacggtcttt
      241 tgagcttcgg cgagctcgct cggcttgagc gcgcgcacga gccgctgcac cgagaagccg
      301 gtgtcgtcgg gtcgcgtcgg tagcttggcg gaagcgtagc ggagctgggc ggcatagaag
      361 agtttgcccg ggccgttcac ggcgaaggtc agcgggcctc gcttttggcc gagcttcgac
      421 gcggggatct ggaacggcac ttcgatcgct gagctgccgt ggaagctcgc attgccgagc
      481 agatcgtcac ccaagaagac ctgcgcgtcg aagttcggct ccgcggtttc ttggttcttt
      541 cggtagtcgt cgagcgcgag caaggcccag gtgttttctt gagtcgagcg gaaggcgccg
      601 tgctcccggc gcgagagcag gcctcgggcg aggcgcgctg cgagcggatg gtgcgggtcc
      661 atcgcgagga aggcgcgcag aaccaacgcc gtcgtgcgaa tcgacgagtc caaccacggg
      721 gcgtaatcgt cgctttggtc gtcgacgaac gcctcgttcg ctccgacgcg aaggtgattg
      781 gtgagctcgg tcgccaagat gttctgcgtt gcgcgcggca tcttcgctcg agccatcgca
      841 tggaggagaa gggcttgcgt gaacagcggc ttgtcgcggc gcgcgtcgaa gagctgattc
      901 aaagttcccg gttgcgcctg tccgacggtc gccaagacgt cggctacgaa cgcgacgtca
      961 gcgtagctgc cggcgcgcgt tcgctcttca tcgagcagat ctcggggctc gacgtcagcg
     1021 tcatcgtcgg gcgcgttgtt ggtgaaccga gcgctcgaga cgactctctg caggtaggcg
     1081 atggcctgat cgcgcgccga cttcggtacg tagaagcgct tgtcggccgc ggtctcgagc
     1141 gcgagcactg catacgcgga gagccaaggc acgtccgccc agtcgccgtc ccagtaccca
     1201 aacccgcccg agttcgtttg atgactcaag atctcgccga gcgcatcgtc gaccacgtcg
     1261 tccaccttcg ctggcatgcg gacgtggaaa gcccgagcca gatcggagag gagcaagagc
     1321 ggcagggtcc ggctgacgag ctgttccgtg caaccgtagg ggtattctag gtttcgttcg
     1381 aaagcctcgc cgattcccgc cagcgcggtc gaagccaggt gcacgtcgag ccctccggac
     1441 tccggacgca cgtcaccgag attgccgagt tcgacggcgg cggctcggct cgtctcgccg
     1501 tacaccgcga ccgcctcgcg cgagacgggc agctcgactt gacgcttcac gagcacgctg
     1561 tcgcgctggt ttgctccgtt cactccaaac tcgagagaca cgggtccgct cttttgagcc
     1621 ttgacgggaa accgaacctc gacgctgcgt ccgcgaggca cggtgacttg tcgcgctgcg
     1681 gggccgctcg caacgagccc ggtcgcgcgc agggtgacgc tcacgttgct gtcgggtagg
     1741 tccttggacg aaacgacgac gccggcctcg aattcgtcac cttcgcgcac gatccgcggt
     1801 agcgcaggcc ttgccatcag ctttcggctc gtcgtgatgc gcgcctctcc gaaaccgaac
     1861 cgctcgtctt cggccgaagc gatcgccatc aagcggaagg tcgtgaggtt gtccggcagc
     1921 ttgaagcgaa agtgagtcgt gccttcggcc gaggtgacgc gccccgcttc gaagaaggca
     1981 gtcgtcttga agtcggcgcg cacggcgtct tctccaccgc cgccaccgtc gtaccccttg
     2041 tcgttgccgg cggggttcaa gaagtcgtag cccaacgttc ggacgcgctc gccgtttttc
     2101 attgccagaa tcttggcgag gctctcgcgg ttctctaggc cgaaaacggc gagctttcgc
     2161 tcggtcgtga acgagggaag cggatccggc gtttgatatc cggtcagcat cagcacgccc
     2221 tcgtcgacgg cgtagaaggt gacggagctt cgcccccgac gcccgtccgt gccgctcacg
     2281 ctgaggtcgg cgtcgatgat gtctccgggg cgatagtcgc tgcgcg
//
LOCUS       DASTKS010000590         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_95308,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000590 DASTKS010000000
VERSION     DASTKS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95308"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFQ35_12180"
     CDS             <1..974
                     /locus_tag="VFQ35_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931445.1"
                     /translation="QDYRLALGLVPGLESGVPFYDAFSSYERADVEAAPGTDVLRRAN
                     MVQRGLQSWTRTYVLKQAYKVLFDRDLDEEDPTRVPSSTPFRRRTVGDEVKEFLESRL
                     PERLASETGGHDLVAWVEWLMKNGEIGLDGRPVGTNTYAEMVAGYERRLTDYQARIEA
                     GEARGKNVRAERQRMLDVQREYGSFKSRVEALRGAAQESSSLYAKASRDYSEALWQSA
                     AWFAAILACWGVAHSFGGRLPPTRRFARWLLPVAFVVVPLALVVVPFWVLANPARAAQ
                     VAGRPLRAVRLLDFEAGEQPVTRGAAAQLEKTRPWTLLKSAAQWRLP"
     gene            959..>3574
                     /locus_tag="VFQ35_12185"
     CDS             959..>3574
                     /locus_tag="VFQ35_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931446.1"
                     /translation="MEAPVIVHRSAWLFLGCAVLGGACAKQTVRSAASPPPLVARVTT
                     EQPRAAKNVAWGIPEFFEVGRRIQVSPNAPPVDTTGWPNAILQAAHRGPRAYLAAIAD
                     EQKQVFVYSEPDPSGRAHRFAMVLPEPRRFSTNGAATAYWGRDPAFMNFALEGARSFA
                     GFVSAGAGGLRHARTTRARFGTGGVNPQDLENVYDLDGSRLTVLEHKGLVPGAPLSFL
                     EYSVYDRDATADDAEPEPVDATELGMLTVALTPLREISWQERRSFESNGYRVVLVTGA
                     HEQSYFLAAPVALEPRWSFGEAPTDLGPYVGSPSEATEAGPGSAAALLIPHAAQGSLL
                     SGVVRDASEVAALLERGGRFVAELAAARNELFRGNTAAYSAYAARALVVAHRSAREPR
                     ALTEFTYGDAPRPSNAADFSAALRYGDAASALRGLVAVHRATPTPELRTAIGDIAESS
                     LQALTRTGATLSRRFDQMALVTEHDDSDGRSDVFIDNGAAAVSVNHRRLVLSSGQSRA
                     PAVTWGALGFTVDGAAASVDEARFSFGIDGSTLPSVVRSDQAALSVSRLFTPSTGSIQ
                     IRETAELRRRTPAVGVRYRFENRGPRPATVNDARITLADFLEYGAGANERSQNRYGLS
                     RVVDGVPLPVGFWMENMSAPLWGDNFAAGEVDLTESYRRLGSRFLVVYGFDKAQVYFL
                     TRRADQVILHNVRSQTKGGFDGFTSLEVRYHLDTTVEPHASLDLPEVLSYTLRAPLHS
                     ADGDGIPDQLQELAAMWTRVVTGQDLAGGPKDAALETDSQHVGLVYSWILAADALEGR
                     SASPLPGREGIANALSEKLRRAALKGATFALGAVNQLRNAREFSSTYANGDDYGFHLA
                     IFDWAYRRTCDARFRD"
BASE COUNT          569 a         1187 c         1205 g          613 t
ORIGIN      
        1 atcaggacta tcggctcgcg cttgggctcg tgcccgggct cgagtcgggc gtcccgtttt
       61 acgacgcttt cagctcttac gagcgagccg acgtcgaagc ggctccgggc accgatgtcc
      121 tgcgtcgcgc caacatggtg caacgcggtc tgcagagttg gacgcgtacg tacgtgttga
      181 agcaggcgta caaggtgctg ttcgaccgcg acctcgacga ggaagatccg actcgagtgc
      241 cgagttcgac gccgttccga cggcgcacgg tcggagacga ggtgaaagag ttcctcgagt
      301 cgcgcttgcc cgaacggctc gcgagcgaga caggtggcca cgacttggtc gcctgggtcg
      361 aatggctcat gaagaacggc gagatcggcc tcgacgggcg ccccgtcggc acgaacacgt
      421 acgcggagat ggtcgccggc tacgagcgga ggctcaccga ctaccaggcg cgcatcgaag
      481 ccggcgaagc gaggggcaag aacgttcgtg ccgaacgaca gcgcatgctc gacgtgcaac
      541 gcgagtacgg atccttcaag tcgcgcgtcg aggccctgcg cggggccgcg caggagtctt
      601 cttcgctcta tgccaaggcg agccgcgact actccgaggc gctctggcaa agcgccgcct
      661 ggttcgctgc gattctcgcg tgctggggcg tcgcgcactc gttcggaggc cgccttccgc
      721 cgactcggcg ctttgcgcgc tggcttctgc ccgtcgcgtt cgtcgtggtg ccgctcgcgc
      781 tggtcgtcgt gccgttttgg gtcctcgcga atccggcacg agcggcgcaa gtggctggtc
      841 gtcccctgcg ggcagtgcgg ctcttggatt tcgaagccgg agagcagccg gtgactcgag
      901 gtgcggccgc ccagctcgaa aagactcggc cctggacgct cctcaagagc gcggcccaat
      961 ggaggctccc gtgatcgtgc atcgctccgc gtggttgttc ctgggatgcg cggtgctcgg
     1021 cggcgcatgc gcgaagcaaa cggtgcgctc cgcagcttcg ccgccgcctc tcgtggcgcg
     1081 cgtgacgacg gagcaacctc gggccgcaaa gaacgtggcc tggggaattc cggagttttt
     1141 cgaggttggg cgccgaatcc aagtttcgcc caacgcaccg cccgtcgata cgacagggtg
     1201 gcccaacgcc atccttcaag ccgcccatcg tggtccgcga gcatacctag ccgcaatcgc
     1261 cgatgagcag aagcaagtct tcgtctactc cgagccagac ccgagcggcc gagctcatcg
     1321 gttcgccatg gtcttgccgg agccgaggcg tttcagcacc aacggcgccg cgacggcgta
     1381 ttggggtcgc gatccagcgt tcatgaactt cgcgctcgaa ggcgctcgta gctttgcagg
     1441 gttcgtgtcc gcgggtgccg gcggactgcg acatgctcga acgacacgcg ctcgcttcgg
     1501 gaccggaggc gtcaaccctc aggatctcga gaacgtctac gatctcgacg ggagccgact
     1561 cacggtgctc gagcacaagg ggctcgttcc gggcgcgccg ctctctttcc tcgagtactc
     1621 ggtttacgat cgcgacgcga ccgcggacga cgccgaaccg gagccagtcg acgctacgga
     1681 gctcggcatg ctgacggtcg cgctcacgcc gcttcgagaa atctcgtggc aggagcggcg
     1741 ctcgttcgag agcaacgggt atcgggtggt cctggtgact ggcgcgcacg agcagagcta
     1801 cttcttggcc gctcccgttg cgctcgagcc gcgctggtcc ttcggcgaag ccccgacaga
     1861 tttggggcct tacgtcggga gcccgagcga ggcgaccgaa gccgggccgg gctctgcggc
     1921 ggcgctgctg attccgcacg cggcgcaggg ctcgttgctc tctggagtcg tgcgcgacgc
     1981 gagcgaagtg gcggccctgc tcgagcgcgg aggccgtttc gtagcggagc tcgcggcggc
     2041 gcgtaacgaa ctcttccgcg gaaacactgc ggcgtactcc gcgtacgcgg cgcgcgcgct
     2101 cgtcgttgct caccggagcg cacgagagcc acgggcgctc acggagttta cctacggcga
     2161 cgcgccgcga ccgagcaacg ccgccgactt ctccgcggcg ctccgctacg gcgacgcggc
     2221 gagcgccctt cgcggtctcg tcgccgttca ccgagcgacg ccgactcccg agcttcgaac
     2281 cgccatcggc gacatcgccg agtcgagcct gcaagcgctc acgcggaccg gcgcgacgtt
     2341 gagccgccgc ttcgatcaga tggcgctcgt gaccgagcac gatgacagcg acgggcgctc
     2401 ggatgttttc atcgacaacg gtgcggccgc ggtcagcgtc aaccatcgcc gcctcgtgct
     2461 gagcagcggg cagtcgcgag ccccggcagt cacctggggc gcgctcggct tcacggtcga
     2521 tggcgccgca gcgagcgtgg acgaggcgcg cttcagcttc gggatcgacg gatcgactct
     2581 tccatccgtc gtgcggtccg atcaggctgc gctcagcgtg tcgcggctgt tcacgccgag
     2641 cacgggaagt atccaaattc gtgagacggc ggagcttcga cgtcgaacac cggccgtggg
     2701 tgttcgatac cgcttcgaaa atcgcgggcc tcggccggcc accgtcaacg acgcgcgcat
     2761 cacgctggct gacttcttgg agtacggagc cggcgccaac gaacgctcac agaatcgtta
     2821 cgggttgagc cgcgtggtcg acggcgtccc cctccccgtc ggcttctgga tggagaatat
     2881 gtcggcgccg ctctggggcg acaacttcgc cgcgggagag gtggatctca ccgagtcgta
     2941 tcgccgtctc ggctcgcgtt tcctcgtcgt ctacgggttc gacaaggccc aggtctactt
     3001 tctgacacgc cgcgcggacc aggtgatctt gcacaacgtt cgctctcaga cgaagggcgg
     3061 cttcgacggc ttcacgtcac tcgaagtccg ctatcatctc gataccacgg tcgagccgca
     3121 cgcctcgctc gacctccccg aggtcctttc gtataccttg cgcgcgccgc tgcattcggc
     3181 agacggggac gggatccccg accagctgca ggagctagcg gctatgtgga cgcgcgtcgt
     3241 cacgggacag gatctcgcag gcgggccgaa agacgcggcg ctagagaccg actcgcagca
     3301 tgttgggctc gtctactctt ggatcctcgc ggcggacgcg ctggagggtc gcagcgcgtc
     3361 accgctaccc ggtcgagagg gcatcgccaa cgctctctcg gaaaagctac ggcgagccgc
     3421 tctcaaggga gcgacgttcg cgctcggcgc ggtcaaccag ctacgtaacg cgcgtgagtt
     3481 ctcgagcact tacgcgaacg gcgacgacta cggcttccat ctcgccatct tcgattgggc
     3541 ctaccgacga acctgcgatg cgcggtttcg cgac
//
LOCUS       DASTKS010000591         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_541484, whole genome shotgun sequence.
ACCESSION   DASTKS010000591 DASTKS010000000
VERSION     DASTKS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541484"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1636)
                     /locus_tag="VFQ35_12190"
     CDS             complement(137..1636)
                     /locus_tag="VFQ35_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931447.1"
                     /translation="MPGQSGGAPAMNALTIRFEGVPIRLGPRIDAQITVRVEPPGAYA
                     VRFSLLDAAAGAVLNRDQVVSGPDGRASVILTTPNSPTHFVLRAQVDSFVATTAISIE
                     GGALTTLNVLPEYSGKRAVSTWTANAYPNTTCGAFQGQPSDGPYLVSAPKGSALRLAD
                     VPAIAPLAVVARAGGVAQGCTTVDNPTPNGETTVGITATDVPLNLGATVLDVVFGASA
                     DDATLKAELQAGASLVQASMRGPDDNEAAALLDAMAAKLEGAELAQFTLARQDAGWDQ
                     SLAAALGRNGGTRIADALARWLRDGQRELLSSHAIQGRISATPMSSDVPHLVVQRIGG
                     ASAASVGVSAVGAKWSVDPNDTLAISASLSWPAASLACAVLSGAASAETGSSELGVAL
                     SRALSCSAVGSHLTTSNAEAALTWSTACDAECNAELCQSALTSSITQACAASKKELST
                     LAVLATGSAEVGSAAQATSFEGSWVGRLTRGTQKSSTSGALLGSAPRGL"
     gene            1894..3312
                     /locus_tag="VFQ35_12195"
     CDS             1894..3312
                     /locus_tag="VFQ35_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9931448.1"
                     /translation="MPSIVPPPLKVLVVEDEPGLREMLGILFRREGYEVVLAPGAMSA
                     IEAIQQHPQPFPVVLTDLAMPDGSGLDVLAAAKTRSSATEVILITAHSSLENAIAAMR
                     LGAYDFVAKPFQSGELAALVAKALEKQAIVAENLRLRAQMRDPDSREIIGRSPALRRV
                     MDLVERVAATRTTVLITGESGTGKERVARAIHRLSERASGPFLVVNSGALPEALMESE
                     LFGHEKGAFTGAQTKHRGLFREAEGGTILLDEVGELPANLQVKLLRVLQERAVRPVGS
                     TQEVPVDVRVLAATNRDIEADVAAGRFRSDLYYRLNVIRIELPPLRDRREDVSLLAER
                     FVRHFAEEMGKDIAGFTPDALRALQTYPFPGNVRELENVIERAVALAGSRVIGLGDLP
                     TELSGTAGGPTPALLALPREGVRLDEVLGEVERRLILEALDRTGGVRKRAADLLGITF
                     RSLRYRLKKLGLGEDSEEDGFD"
     gene            3422..3988
                     /locus_tag="VFQ35_12200"
     CDS             3422..3988
                     /locus_tag="VFQ35_12200"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9931449.1"
                     /translation="MFLPQRILGTRRSRKAQRGFTLTELMIVVTLVGILASVGIASFR
                     KRVAASKTTEAASVVQAIRGAEEAFRSENQVYLNVSSVGAWYPSKGQGSTITTWEQKT
                     HTDLAAWQRLGARVTQPVQFGYLVNAGRAGDALPALNIDDKSVNLGKPTDAWYLIQAR
                     SDSDKDGVYCNVLANSFTPELYVEHEGE"
     gene            4026..>4517
                     /locus_tag="VFQ35_12205"
     CDS             4026..>4517
                     /locus_tag="VFQ35_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931450.1"
                     /translation="MSKTRESDGGATRSAWGVYAQGLFAVYRTLGLLVLAAFGMTCVA
                     LWIHHRADVLPSSYTVFEPLRTLKGVLELPGFMVAVLVTFWVGRLRLRGEWKLDAIRA
                     AFTQRVALPDALPGLGRRAGLTALVLVADALVLDGQDVLDFLALGVLAFVASKERRGL
                     TEWL"
BASE COUNT          713 a         1469 c         1537 g          798 t
ORIGIN      
        1 accaatccga cgtcccgccc acactaccgg cggtacgtcg aatgctcgtg cgctggttgg
       61 ttgcgcctgt gccgtgtccg aagtgcgcgc accctgtgcg ccctttcgtg cctatctaga
      121 aagtggcgga gtagtgctag aggccgcgcg gcgcgctgcc gaggagcgca cccgaagtgg
      181 acgacttctg cgttccccgt gtgaggcgcc cgacccagct tccctcgaag ctcgttgctt
      241 gcgcggcgct gccgacctcg gcgctaccag tcgcgagcac cgcaagcgtg ctgagttctt
      301 tcttcgaggc ggcgcacgcc tgggtgatcg agctcgtgag cgcgctctgg cagagctcgg
      361 cgttgcactc cgcatcacaa gccgtcgacc aagtcagggc cgcctccgcg ttgctggtcg
      421 tgaggtgact accgacggcg ctacagctca acgcgcgaga cagcgctacc ccgagctcgc
      481 tcgaaccggt ttcggccgaa gcggcgccgg agagcaccgc gcacgcgagg ctcgcggcgg
      541 gccagctgag cgaagccgaa atggcgagag tgtcgttcgg atcgacgctc catttcgcgc
      601 cgacggcgct cacgccgacg ctcgccgccg acgcaccgcc gattcgttgg accacgaggt
      661 ggggaacgtc cgacgacatc ggagtcgcgg aaatcctccc ttgaatcgcg tggctcgaaa
      721 gcagctcgcg ctggccgtcc cggagccagc gagcgagcgc gtctgcaatg cgcgtcccgc
      781 cgtttcgccc gagcgccgcg gcaaggctct gatcccagcc cgcgtcttgg cgagccaggg
      841 tgaactgagc gagctcggcg ccttctagct tcgcggccat cgcatcgagc agagcggctg
      901 cttcgttgtc atcggggccg cgcatcgacg cttgtacgag ggaggcgccg gcctgaagct
      961 ctgccttcag tgtcgcgtcg tccgcgctcg ctccaaacac gacgtcgagc acggtcgcgc
     1021 cgaggttgag cggaacgtcc gtggcggtga tgccgacggt ggtttcgccg ttcggcgttg
     1081 ggttgtcgac ggtggtgcag ccttgggcca cgccgccggc gcgggccacg acggcgagtg
     1141 gcgcgatcgc gggcacgtcg gcaaggcgga gcgcgctgcc ctttggggcc gagaccaagt
     1201 aggggccatc gctcggctgg ccttgaaagg cgccacacgt cgtgttcggg taggcgtttg
     1261 cggtccaggt gctgacggcg cgtttgccag agtattcggg caaaacgttc aaagtcgtga
     1321 gggctccgcc ttcgatcgaa atcgcggtcg tcgcaacgaa cgaatccacc tgagcgcgca
     1381 gcacgaagtg cgtcggggag ttcggcgtcg tgagaatgac gctggcgcgg ccgtcgggac
     1441 cgctcacgac ctggtcgcga ttgagcacgg cgccggcagc ggcgtcgagg agcgagaacc
     1501 gtacggcgta cgcgcccggc ggctcgactc gcaccgtgat ttgagcatcg atgcggggac
     1561 cgagccggat cggtactccc tcgaaccgaa tggtgagtgc gttcatggcc ggcgcgccgc
     1621 cgctctgccc gggcatgaca gtgccggaat cagccgtgct ttgagccgaa cacgcaggga
     1681 gcccgagcga gcacgcgctc gcgaatacga accccagcgc cgccaaacag gcccactttt
     1741 cgagcctgcg gtgcggcgcg gaccatccag tcggtcgagg aggggctcgc atcggctctt
     1801 ctggctagat tagactagcc cccgacctcc gcaaggcgct cgcggcaacg cgcatagcgc
     1861 cgcacctgag agtcctctac attcttcggc gaagtgcctt ccatcgtccc tccgccgctg
     1921 aaggtcctcg tcgtcgagga cgagcccggg ctgcgcgaga tgctgggcat cctgtttcga
     1981 cgagaagggt acgaagtggt cttggcgccc ggtgccatgt cggcgatcga ggcgattcag
     2041 cagcacccgc agccttttcc ggtggtgctc acggatcttg cgatgcccga cggatcgggc
     2101 ctcgacgtgt tggcggcggc aaagactcgg agctcggcca ccgaagtcat cctgatcact
     2161 gcccattcct cgctcgagaa cgcgattgcc gcgatgcgtc tcggcgccta cgacttcgtc
     2221 gcgaagcctt ttcagtcggg ggagctcgcg gcgttggtcg ccaaggccct cgaaaagcag
     2281 gccatcgtcg ccgagaacct gcggttgcgt gcgcagatgc gcgaccccga ctcgcgcgaa
     2341 atcatcggac gcagtccggc gcttcgccga gtgatggacc tggtcgagcg agtcgcggcg
     2401 acgcgcacca ccgttctcat caccggagag agcggcaccg gcaaggagcg cgtcgcgcgt
     2461 gccatccatc gtctttcgga gcgcgcgagc gggccgttcc tggtcgtaaa ctcgggggcg
     2521 ttgcccgaag cgctcatgga aagcgagctt ttcggtcacg agaagggcgc gttcacgggc
     2581 gcgcaaacga agcatcgcgg gctctttcgc gaggccgaag gcggcaccat cttgctcgac
     2641 gaagtcggcg agctcccggc aaacttgcag gtgaagctcc ttcgcgtttt gcaggagagg
     2701 gccgtgcgtc cggtgggctc cacgcaagag gtgccggtcg acgtgcgggt gctcgccgcg
     2761 accaaccgcg acatcgaagc cgacgtcgct gcgggccgct tccgcagcga tctctattac
     2821 cgcttgaacg tcattcgcat cgaactaccg ccgttgcgcg accgacgtga agacgtgagt
     2881 ctgctcgccg agcgattcgt tcgtcacttc gcggaagaga tgggcaagga catcgccggg
     2941 ttcaccccgg acgcgcttcg cgccctgcag acctacccgt tccccggaaa cgttcgtgag
     3001 ctcgagaacg tcatcgagcg cgcggtcgca ctcgccggct cgcgcgtcat tggcctcggc
     3061 gatctcccga ccgagctgag cggcaccgcc ggcggcccca ctcccgcgtt gctcgctttg
     3121 ccccgcgagg gcgtgcgcct cgacgaggtg ctcggcgagg tggagcggcg cctgattttg
     3181 gaggccttgg atcgcaccgg gggcgtgcgg aagcgcgccg ccgacctgct cgggattacc
     3241 tttcgatcct tgcgctaccg cctgaaaaag ctgggcctcg gcgaagacag cgaagaagat
     3301 ggcttcgact gacgctgccg ctcgctgcga cctccatggc gcgcatgtcg tctggagtgg
     3361 ttcgcgttgg cagttggcgc gactttggta ggttttttcg gagccgtgct aggctttctc
     3421 tgtgtttttg ccgcagcgga ttctcggcac gcgacgcagc cgcaaggccc agcgcggctt
     3481 cacgctgacc gagctcatga tcgtcgttac cctagtcggt attctcgcga gcgtcgggat
     3541 cgcatccttc cggaagcgcg tcgccgcctc caaaaccacg gaggcggcga gcgtcgtgca
     3601 ggccatccga ggagccgaag aggcgtttcg cagcgagaat caggtgtatc tgaacgtctc
     3661 ctcggtcggg gcttggtatc ccagcaaggg tcaaggctcg acgatcacga cctgggagca
     3721 gaagacacac acggatctcg cggcgtggca gcgcctcggg gcgcgcgtta ctcaaccggt
     3781 tcagttcggg tatctcgtca acgcgggccg cgccggtgac gcgctgccgg ctttgaacat
     3841 cgacgacaag tcggtcaacc tcggcaagcc gaccgacgct tggtatttga tccaggcccg
     3901 ctcggactcc gacaaagacg gagtctactg caacgtgctg gcgaacagct tcacgcccga
     3961 gctctacgta gagcacgaag gggaatgagc gcttgctagc ctgaaccgcg gcgccgaatc
     4021 gggtcatgtc gaagacgagg gagagtgacg gcggtgccac gcgctcggct tggggcgtct
     4081 acgcccaggg cctgttcgcc gtttatcgaa ctctcggcct gctcgtcttg gcggcgtttg
     4141 ggatgacctg cgtggcgctt tggatccacc atcgcgcgga cgtcctgccg agctcgtata
     4201 cggttttcga accgctccgc acgttgaaag gcgtgctcga gctgccaggc ttcatggtgg
     4261 cggtgctggt caccttttgg gtgggccgcc tgcgcctgcg gggagagtgg aagctcgacg
     4321 cgatccgagc cgcgttcacg caacgggtgg cgctgcccga cgcgctccct gggttggggc
     4381 gccgcgccgg actcaccgcg ctcgtgctcg tcgcggacgc cctcgtcttg gacggccaag
     4441 acgtgctcga ctttttggcg ctcggcgtgc tcgctttcgt cgccagcaag gagcgacgtg
     4501 gtctcaccga gtggctg
//
LOCUS       DASTKS010000592         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_542110, whole genome shotgun sequence.
ACCESSION   DASTKS010000592 DASTKS010000000
VERSION     DASTKS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542110"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFQ35_12210"
     CDS             <1..420
                     /locus_tag="VFQ35_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9931451.1"
                     /translation="NDLDSLLDTADKCPSQAEDMDGFEDTDGCPDLDNDKDGLPDTAD
                     QCPNDPETKNGYKDEDGCPDEVDTDNDGIPDARDKCPNEAEDTDGFEDTDGCPDPDND
                     NDGVPDEHDECVDEPETVNGFQDEDGCPDVAEGKKRR"
     gene            420..956
                     /locus_tag="VFQ35_12215"
     CDS             420..956
                     /locus_tag="VFQ35_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9931452.1"
                     /translation="MVARGLGGAAVVAALLLGALPGCDAGGGKGAAHAISEHPLLGAA
                     APPLEVPTPDGKGRVALAAHAGKVVVVDFWATWCEPCRQSFPAYQKLVEEFGGKLVVI
                     GVSVDEGPEPIAAFVKATAAKFPIGWDEGQAAAKAYEPPKMPTSFVVDKSGIVRFVHG
                     GYSHGDEKQLREEIKSLL"
     gene            1130..1948
                     /locus_tag="VFQ35_12220"
     CDS             1130..1948
                     /locus_tag="VFQ35_12220"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9931453.1"
                     /translation="MQRLLRSALLIAVVSTALSAPAKPRLDDLTTRLANSDFRVRVQA
                     ALELGRTGDARALSPLVSALDDPSAAVRAAAAAALGKLGDSRGKGALAQHRDDSSNAV
                     RAEIKSALDKLEPKRTSDDEATQVVAQPRKIVVKLAGVHNGTRVKSTNVERDVLAESK
                     RKLGELGVSVVAEEGDASESSAMVKLIPSIQKLQASRDGDSVVYSAKVEYILQTLPDE
                     TIMARLSGSASAAASDAETQDRVAIARLRREVLSAAIQSALSRASNAFQAAARL"
     gene            2012..>2282
                     /locus_tag="VFQ35_12225"
     CDS             2012..>2282
                     /locus_tag="VFQ35_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931454.1"
                     /translation="MSVLPIGHLSRGPAMTAEARIYPDLNQTADELWAGDGQSQTEPP
                     PTAAPQVLRSAGKYSAGSIISGKYQLIEVLGEGGMGSVWLAKNRTL"
BASE COUNT          436 a          750 c          768 g          328 t
ORIGIN      
        1 aacgatctcg attcgttgct ggatacggcc gacaagtgcc cgagccaagc cgaggacatg
       61 gacggcttcg aagacaccga cggctgcccc gacctcgaca acgacaagga cggcctgccc
      121 gacaccgctg atcagtgtcc gaacgacccg gagaccaaga acggctacaa ggacgaagac
      181 ggctgccccg acgaggtcga caccgacaac gacggcatcc ccgacgctcg tgacaagtgc
      241 ccgaacgaag ccgaggacac cgacggcttc gaggacaccg atggctgccc cgatccggac
      301 aacgacaacg acggcgtgcc ggacgagcac gacgagtgcg tcgacgaacc cgagacggtg
      361 aatgggttcc aggacgaaga cggttgcccc gacgtcgccg agggcaagaa gaggcgctga
      421 tggtggcacg gggactcggt ggggccgcgg tggtcgcggc cctgctcctc ggcgcgctgc
      481 cggggtgcga cgctggcggt ggcaaaggcg cggcccacgc gatctccgag cacccgctgc
      541 tcggcgcggc cgcgccgccg ctcgaggtgc cgactcccga cgggaagggc cgcgtggcgc
      601 tcgccgctca cgccggcaag gtcgtggtcg tcgacttctg ggcgacgtgg tgcgagccct
      661 gtcgtcagtc gttccctgcg taccagaagc tcgtcgaaga gttcggcggc aagctggtcg
      721 tcattggcgt gagcgtcgac gaggggccag agcccatcgc cgcgttcgtg aaggcgaccg
      781 ctgccaagtt cccgatcggg tgggacgagg gtcaggcggc ggcgaaggcc tacgagccgc
      841 cgaagatgcc gacgagcttc gtcgtcgaca agagcggcat cgttcgcttc gtgcacggcg
      901 gttactcgca cggcgacgag aagcagctcc gcgaagaaat aaagtcgctc ctttagccac
      961 gagcgctgcc gtcgtcccac aaccgcgcag ctcgcgcgag cttcgcgatc ggtcggagta
     1021 cgccgcactt acgctccgat gctgacaccg aactaagcaa cgacgcctca ggtttgttgc
     1081 gaggtgtgcc gctgttcagc atgatcgttt gaaacgaacg aagtcaccga tgcagcgact
     1141 cctccgctcc gcactgctca ttgccgtcgt atccacggcc ctgtccgccc cggccaagcc
     1201 gcggctcgac gacctgacga cccggctcgc caacagcgac ttccgcgtga gggtgcaggc
     1261 ggcgctcgag ctcggccgaa ccggcgacgc gcgggcgctc tccccgctgg tctcggcgct
     1321 cgacgatccg agcgcggcgg tgcgtgccgc ggccgccgcg gccctcggca agctcggcga
     1381 cagccgcgga aaaggcgcgc tagctcagca tcgtgacgac agctcgaacg cggtgcgcgc
     1441 cgagataaag agcgcgctcg acaagctcga accgaagcga acgagtgacg acgaggccac
     1501 gcaggttgtg gcgcagcccc ggaaaatcgt ggtgaagctc gcgggcgtgc acaacggaac
     1561 gcgcgtcaaa tcgacgaacg tcgagcgcga cgtgctcgcc gaatcgaagc gaaagctcgg
     1621 tgagctcggc gtttcggtgg tggccgaaga gggcgacgcg agcgagtcgt cggcgatggt
     1681 caagctcatc ccgagcattc aaaagcttca agcctctcgc gacggcgact ccgtcgtcta
     1741 ctctgcgaag gttgagtaca tcctgcagac gctgccggac gagacgatca tggcgcggct
     1801 ctctggcagc gctagcgcgg ccgcatccga cgcggagact caagatcgcg ttgcgatcgc
     1861 ccggctgcgg cgtgaggtcc tttcggccgc gatccaaagc gcgctcagcc gcgcatccaa
     1921 cgctttccag gccgcggcgc gcctataaag gccctcgtcg ggcggagggc gcgaaacgtg
     1981 cgcgccgaga gcactttaag tgtcgcaaga agtgtccgtt cttccgatcg ggcacctctc
     2041 ccggggccct gcgatgacag cagaagcgcg catttacccc gatctgaacc agaccgccga
     2101 cgagctctgg gcaggggacg gtcaatctca gaccgaacct ccgcctacag cagctcctca
     2161 ggtactgcgg agcgcaggca agtacagtgc aggctctatc atctccggca agtaccagct
     2221 gatcgaagtg cttggcgaag gtggcatggg gtcggtgtgg ctcgccaaaa accgtacgct
     2281 gg
//
LOCUS       DASTKS010000593         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_318646, whole genome shotgun sequence.
ACCESSION   DASTKS010000593 DASTKS010000000
VERSION     DASTKS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318646"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..788)
                     /locus_tag="VFQ35_12230"
     CDS             complement(276..788)
                     /locus_tag="VFQ35_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931455.1"
                     /translation="MKATPVAFSLILLASTPAFGEPGQARAPHAAPRGSSDGERKVDA
                     TFAAEVLVLHATNTQKGIDARIGAMPELKKPPFSSYDSYELLDRARLTLDRQSPKTLK
                     LANGRVLETRLVEVLGKELVRLSASINQPGGKEFLPLLEVKAKVGQAFIVAGQRYKQG
                     ILVLVLRVTH"
     gene            complement(865..1446)
                     /locus_tag="VFQ35_12235"
     CDS             complement(865..1446)
                     /locus_tag="VFQ35_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931456.1"
                     /translation="MKPDELIMLLHDGELDPEQAEAVRALIEDDASAAALFEQLDQVA
                     AVVRESVLAAPASSDLTDAVMARIEREPVVLDVKRAGAPRRGPGRALAIVSGALALAA
                     GLSLLVTRAMHSESLARSGDVAAPVAPMSAPEPEPPEQSVAIERVDFGGSQGAVFMVP
                     GSDDRTLVIWTLDDAPIWAVDDVSDDKGPEVDL"
     gene            complement(1443..2120)
                     /locus_tag="VFQ35_12240"
     CDS             complement(1443..2120)
                     /locus_tag="VFQ35_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931457.1"
                     /translation="MGVNSGDERASNRLAREADAEVDRALIERAQAGDKSAFRELVER
                     HQRRAFAIAFALVKDEEDAREVVQEAFVRVFRGLDQFQGGSSFFTWLYRIVTNLSIDV
                     IRRPARRDTGLDESIELEESDDAPLARIEGADPLLAVHRTELRSRIESALAELPVYHR
                     GVILMREVQGMSYEEMAEAMGVSKGTIMSRLFHARRKLQKALAECYLDVFDAAPKPEE
                     GEEEGES"
     gene            complement(2314..3441)
                     /locus_tag="VFQ35_12245"
     CDS             complement(2314..3441)
                     /locus_tag="VFQ35_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9931458.1"
                     /translation="MTLRVSAGGFALAAASFLAVTACNGSGKSASPMPSASVLPPSAP
                     QAAVVPVPGNAPTSFADLVAHADPGVVFVKTTHERRALPGRSIIGEALGSAFVYDPDG
                     LILTNNHVIEGATDIQVTVGNSRVLSATVVGRDPPTDVAVLRVEAKGLPALPIGDSDA
                     SRVGDWVIAIGNPFGLSHTVSAGIISAKDRTNQDLNRSGGGLSEEGYYDFLQTDASIN
                     PGNSGGPLLDTQGRVVGINTAIRGGANNIGFAIPINMVKEVLPRLLKDGKIQRSALGV
                     VVSPVLPDDVERLKLGAPGGALVNSVVAGGPADRAGLAIDDVILAFNGRSILRPEHLR
                     WSASLAGVGSSATIRASRRGRQFDIKVTLGELPAPAVPSER"
     gene            complement(3438..3779)
                     /locus_tag="VFQ35_12250"
     CDS             complement(3438..3779)
                     /locus_tag="VFQ35_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET9931459.1"
                     /translation="MAQGTNDERELDPGQAEFEEARKAANAGAELPAIDLTTLILSFS
                     HNVLVHLGDAADPVTGEREVDLPLARQNIELIALLQEKTRGNLSGEEERVLGQALYDL
                     RMRYVEVVKRK"
     gene            complement(3782..4246)
                     /locus_tag="VFQ35_12255"
     CDS             complement(3782..4246)
                     /locus_tag="VFQ35_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931460.1"
                     /translation="MPMPEHFKFDVRVRERLQRKDQLSETELEQHLGALPDVSANAVD
                     IELKQPALQTEAERAENSVIVRPAPPRPVMVAPIPIEDEEDEEDELPVKPEKRVSAAP
                     AAAAAPSVDEEEDDEDEEDDEDEDDEDDEDDEDEDEGGGEKAPGADEGDSGV"
     gene            complement(4264..4869)
                     /locus_tag="VFQ35_12260"
     CDS             complement(4264..4869)
                     /locus_tag="VFQ35_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9931461.1"
                     /translation="MSDSKRQRSFQAGPGMWVRLKYRAFDEDDEPVEQLEQEIEYVHG
                     YGALLPELETALDGQGEGAHRTVRLDQKRAFGPRRNDRIVEFDRAEFPPELAAGDHFD
                     AEGEDGHVLVLRVLEVRDDAVVVDLNHPLAGQDVRFELEVLGVRPATPEEIELAEAAL
                     QAEGPVDGSLISAERLLRGPSRGYEMGPSAPPRPDGTEGEN"
     gene            complement(4866..5003)
                     /locus_tag="VFQ35_12265"
     CDS             complement(4866..5003)
                     /locus_tag="VFQ35_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931462.1"
                     /translation="MTFSSVINLNLSGRMGRGSRFVSSRFLLAASAPSGRLAHPKPER
                     A"
     gene            complement(5084..5251)
                     /locus_tag="VFQ35_12270"
     CDS             complement(5084..5251)
                     /locus_tag="VFQ35_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931463.1"
                     /translation="MVEDRKRPQPKPSSTLEARARRDRPEEDELVGAILAALEHGVEA
                     PPIKSVADRGA"
     gene            5534..>5787
                     /locus_tag="VFQ35_12275"
     CDS             5534..>5787
                     /locus_tag="VFQ35_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9931464.1"
                     /translation="MEIPGGAFSAGSVPGEEGRVPELEPRLRKVELGPYQIDRLPYPN
                     DPAQPPKTGVSRDDAKRLCAERGGRLCTELEWERACKGPE"
BASE COUNT          966 a         1976 c         1832 g         1013 t
ORIGIN      
        1 gagcgcatgg cggagatcgt acgtaaggtc ggccggatca cgaagtacga aacgaagaac
       61 tacgtgggcg acgccaaaat cctggatttg gaaaaggcga gcgagagcaa caagcccggc
      121 agtctcggag gctgacgcgc cagcctgtcg gcgctcgctg cgcgaggagt gagcaccgga
      181 gaaaggctga agcccttcga ggagcggagc gacgaggccg tgcttcgcgg ccgcaggatc
      241 ttggctcgca gcagggcggg gaacttggga cgcccctagt gcgtgacgcg caaaacgagc
      301 accaggatgc cctgcttgta gcgctggccc gcaacgatga aggcttgccc gaccttcgct
      361 ttgacctcca gcagcggcaa aaactcctta ccgccaggct ggttgatgct cgcggagagc
      421 cgaaccagct ctttgccgag cacctcgacc aagcgagtct ccaagacgcg gccgttcgcg
      481 agtttcaagg tcttgggtga ttggcgatcg agcgtcaggc gcgcgcggtc gagcagctcg
      541 tagctatcat acgaggaaaa tggcggcttc ttgagctccg gcatggcgcc gatgcgagcg
      601 tcgatgccct tttgcgtgtt cgtcgcgtgc agtaccagca cctcggccgc gaacgtggca
      661 tcgactttcc gctcgccatc gctcgaaccg cgaggggctg cgtgcggcgc gcgtgcttgc
      721 ccgggctcac cgaatgcggg agtcgaagcc aacaagatga gtgagaatgc gaccggcgtc
      781 gccttcatct tgggacggcg ctttccacgg acgctcgagg cagacggcgc gctctttctt
      841 tcgaatgcga gtgccgacta ggcttcatag gtccacctct ggacctttat catctgaaac
      901 gtcgtcgacg gcccaaatcg gcgcgtcgtc gagcgtccaa atgactagcg tccgatcgtc
      961 ggacccgggc accatgaaga ccgcgccctg acttccgcca aagtccaccc gctcgatggc
     1021 caccgactgc tctggcggct cgggctccgg cgccgacatg ggcgcgaccg gcgcggcaac
     1081 gtcacccgag cgcgcaagcg actctgaatg catcgcgcgc gtgacgagca gggacaaccc
     1141 cgcggcgagt gcgagcgcgc ccgaaacgat cgcgagagct cgcccgggcc ctcgacgagg
     1201 agcacctgcg cgtttcacgt cgagcaccac cggctcgcgt tctattcgcg ccatgaccgc
     1261 gtcggtaaga tcgctcgacg ccggtgcggc gaggaccgat tcgcgaacca ccgctgcgac
     1321 ttggtcgagc tgctcgaaca gagcggccgc gctcgcgtcg tcttcgatga gggcccgcac
     1381 cgcttcggct tgctcggggt cgagctcgcc gtcgtgaagc agcatgatga gctcgtcagg
     1441 cttcatgact cgccttcctc ttcaccttct tcgggtttcg gggccgcgtc gaacacgtca
     1501 aggtagcact cagcgagcgc tttttggagc ttccgcctag cgtgaaagag ccggctcatg
     1561 atggtgccct tcgagacgcc catcgcctct gccatttcct cgtagctcat cccctgcacc
     1621 tcccgcatga ggatgacccc tcggtggtag acgggaagtt cggcgagagc ggattcgatt
     1681 cgcgagcgca gctcggtgcg gtggacggcg agcaacgggt cggcgccttc gatgcgtgca
     1741 agtggggcat catccgactc ctcgagctcg atgctctcgt cgagcccggt gtcccggcgc
     1801 gccggcctgc gaatgacgtc gatcgagaga ttcgtgacga tacggtagag ccaggtgaag
     1861 aagctcgacc cgccttggaa ctggtccagg ccacggaaca cccgcacgaa cgcctcttga
     1921 acgacctcgc gggcgtcctc ctcgtccttg acgagggcga aagcaatggc gaaagcgcga
     1981 cgctggtggc gctccaccag ttcacgaaag gcgcttttgt cgccagcttg ggcacgttcg
     2041 atgagggcac gatcgacctc cgcgtcagct tcgcgggcca accggttgga cgcgcgttcg
     2101 tccccgctat tcacgcccat gacgtcgaag tctcgcgccc agctcgccaa cgaggcgaaa
     2161 ggaccaagcc ggaccgcttc gcccggccga gcacagtggg cgaccgagga ttcggctgct
     2221 tgggccatag cacgatcgac gagccgctta cggtttcgat gcaatcccga aaccgggccc
     2281 ctgcgatttt cgcccccgag cgggccatcg gggtcaccgc tcgctcggca ccgccggcgc
     2341 ggggagctcc cccagagtga ctttgatgtc gaactggcgg ccgcgacgcg acgcccgaat
     2401 cgtcgccgag ctccctaccc cagccaagct cgctgaccag cgaaggtgct cgggacgtaa
     2461 aatggagcgg ccgttgaacg ccagaatcac gtcgtcgatc gcgagcccag cgcggtctgc
     2521 cggaccgccg gctacgaccg aattgacgag cgccccgccc ggagcgccca atttcaggcg
     2581 ctcgacgtcg tccggaagca ccgggctcac cactaccccg agcgcgctcc gctgaatctt
     2641 cccgtccttc agcagtcgcg gcagaacttc cttcaccatg ttgatgggga tcgcaaagcc
     2701 gatgttgttg gcaccgccgc ggatggccgt gttgatgccg accaccctcc cctgtgtgtc
     2761 gagtagcggc ccgcccgagt ttccgggatt gatgctcgcg tccgtttgga gaaagtcgta
     2821 gtacccctcc tcgctgagcc ctccgccgct gcgattgagg tcctggttgg ttcggtcctt
     2881 cgccgagatg atgccggcgg aaaccgtatg gctgagcccg aacggattgc cgattgcgat
     2941 cacccagtcg ccgacgcgag acgcatccga gtcaccgatc ggaagagccg gcaggccctt
     3001 cgcctcgacc cgcagcacgg cgacgtccgt tggaggatct cgccccacga ccgtcgcgct
     3061 gagaacgcgg gagttaccca ccgtgacttg aatgtcggtc gccccctcga tcacgtggtt
     3121 gttcgtgagg atcagaccgt cggggtcgta cacgaacgcg ctgcccagcg cctcgccgat
     3181 gatgcttcgc ccgggcaggg cgcggcgctc gtgggtcgtc ttcacgaaca cgactccggg
     3241 atcggcgtgc gcgaccaaat ccgcgaagct cgtcggggcg tttccgggca ccggcacgac
     3301 cgcggcttgc ggcgcactgg gtggcaacac cgatgcgctc ggcatcggcg acgcggactt
     3361 accgctcccg ttacaggcag tgacggcgag aaagcttgcc gccgccagcg cgaacccgcc
     3421 cgccgaaacg cgcaacgtca cttgcgcttc acgacctcga cgtaacgcat ccgcaggtcg
     3481 tagagcgcct gaccgagcac gcgctcttcc tcaccgctga gattgccgcg cgtcttttcc
     3541 tgaagcagcg cgatgagctc gatgttctga cgggcgagcg gcaaatcgac ctcgcgctcg
     3601 cccgtcacgg gatccgcagc atcgcccaag tgaacgagca cgttgtgact gaacgagaga
     3661 atcagcgtcg tgagatcgat ggccggcagc tccgcgccgg cgtttgcggc cttgcgggct
     3721 tcctcgaact ccgcctgacc cggatcgagc tcgcgttcgt cgttcgtgcc ctgggccatg
     3781 atcagactcc cgaatcgccc tcgtcggcgc ccggcgcttt ctcgccgccg ccttcatcct
     3841 cgtcttcgtc gtcctcgtcg tcttcgtcgt cttcgtcctc gtcgtcttcc tcgtcctcgt
     3901 cgtcttcttc ctcgtcgacc gacggggcag cggccgccgc cggggcagcc gagacgcgct
     3961 tctcgggctt caccggaagc tcgtcctctt cgtcttcctc atcttcgatc ggaatcggcg
     4021 cgaccatcac cgggcgcggc ggagcgggcc gcacgatgac gctgttttcc gcgcgctcag
     4081 cctcggtctg caaagcgggc tgcttcaact cgatatccac ggcgtttgcc gacacgtcgg
     4141 gcaacgcgcc caagtgctgt tcgagctcgg tctcgctgag ttggtcttta cgctgcaggc
     4201 gctcgcgaac gcgaacgtcg aacttgaagt gctcgggcat cggcatgtgg aatttcttct
     4261 ccgtcagttc tcgccttcag tcccgtctgg gcgcgggggt gctgaaggac ccatctcgta
     4321 gccccgcgat ggcccccgaa gcaagcgctc ggccgaaatc aaagaaccgt caaccggccc
     4381 ctcggcctgc aacgcggcct cggcgagctc gatctcctcg ggcgtcgcgg gccgcacgcc
     4441 tagcacttcc agctcgaaac gcacatcctg tccagcaagg gggtggttca agtcgaccac
     4501 gaccgcgtcg tcgcgcacct cgaggactct gagaacgagg acgtgaccat cctcgccctc
     4561 cgcgtcgaag tggtctcccg ccgcgagctc aggagggaac tccgctcggt cgaactcgac
     4621 gatgcggtcg ttcctgcggg gaccgaatgc ccgcttctgg tcgagccgca cggtccgatg
     4681 agcgccctcc ccttgaccat cgagcgcggt ctcgagctct ggcaggagcg ccccgtaccc
     4741 gtgcacgtac tcgatctctt gttcgagctg ctcgacgggc tcgtcgtcct cgtcgaatgc
     4801 gcggtacttg aggcgaaccc acatgccagg gcccgcctgg aacgagcgtt gtcgcttgga
     4861 atcgctcacg cgcgctccgg tttaggatgc gcgagacgac cggagggcgc gctcgccgcg
     4921 agcaggaagc ggctcgagac gaaccggctg ccacggccca tgcgacccga gaggttcagg
     4981 ttgatgacgc tgctgaaggt cacttgctgt cgccgagtcg atacgccagg tcccgctcaa
     5041 atgcacgccg caagacgacc gccttgctcg agaatagctg cgttcacgcc ccacgatctg
     5101 cgacgctctt tattggggga gcttcgaccc catgctccaa cgcggccaaa atggctccga
     5161 cgagctcgtc ttcttcgggg cggtcgcgcc gcgctcgcgc ttccagcgtg gagctgggct
     5221 tgggttgtgg gcgcttgcgg tcctcgacca ttttgagctc acagagcttt ccatcccgta
     5281 ggcacgcgga caagctccgc cgcgccggtt tcagttgact cggacgcctc gcgcgtcttc
     5341 tgctcccggc gatcgcctca tgctggagca tttcgtccga tctgcgcggc tcttactgcc
     5401 gtgcgtttgt gtactcgcgg cgtgcaagcc gaaatccaac gcgcccaccc gccctgcgcc
     5461 ggcaagctcc gcctccagca gcccggccac gcccctgcgc gcacccaaag aagcgcctct
     5521 ctccggagag cgcgtggaga tcccaggcgg cgccttctcg gcgggaagcg ttccggggga
     5581 agaagggcgc gtgcccgagc tcgagccgcg cctgcgaaaa gtcgagctag gcccgtacca
     5641 aatcgaccgg cttccctatc cgaacgaccc cgcacagccc cccaagacgg gggtgagccg
     5701 cgacgacgcc aagcgcctgt gcgccgagcg cggagggcgg ctctgcacgg agctcgagtg
     5761 ggagcgcgcc tgcaagggac cggagag
//
LOCUS       DASTKS010000594         5799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_987650, whole genome shotgun sequence.
ACCESSION   DASTKS010000594 DASTKS010000000
VERSION     DASTKS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987650"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFQ35_12280"
     CDS             <1..546
                     /locus_tag="VFQ35_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931465.1"
                     /translation="DFEHEDSYSVSLGASPLVNPKSSVAATLGTAQPLSGMQELRVDF
                     EYRNESTAWTQWMYFPLRFDRTRDFKSLAGVRFLARADSARTLRVDLESIVYQAGNEG
                     IKFGWEVPVGPEATAVEVRFADAKLPSWAHATTDVLAAVLAKLDGLSFHPFCAGRDQS
                     GQLPDGTSDAGHLELDDIEFF"
     gene            complement(562..1305)
                     /locus_tag="VFQ35_12285"
     CDS             complement(562..1305)
                     /locus_tag="VFQ35_12285"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET9931466.1"
                     /translation="MAHPAFERTAHRPWPLPTTKWSWRQTWCDLLFAHWPVPVSVLRP
                     LVPPDLEIQEFDGTSWVGVVPFRMEGVMRRPLPNMPWVSAFPELNVRLYVERDGKPGV
                     WFLSLDATNPLAVWAARRFFFLPYYRAAMSVRVEQEFIHYGSKRAGAEFLGSYRPTSE
                     TYLSEPGTLEHFLTERYCLYALSPNGALVRNEVHHVPWPLQRAELRLQTNTMLDFHGI
                     AVSGPPPHLHFARRLEVVVYPGELALPPP"
     gene            complement(1420..2202)
                     /locus_tag="VFQ35_12290"
     CDS             complement(1420..2202)
                     /locus_tag="VFQ35_12290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931467.1"
                     /translation="MTTFAVVTGASSGIGKELAKLFAADGHGVVLVARRKDELDALAR
                     ELSAVRGVPAIVIARDLGQRESPSAIFEAVRAQSLDVEFLVNDAGFGSNGAFVEQDVE
                     RELQMVDVNVKALVELTYLFLPSMFSRGGGRILNIGSTSGFQPGPFMATYYATKAFVN
                     SFSEALAFELKGTGVTVTLSCPGATATQFSSSAGTDNTPPFRAAVAEPIDVAREAYRA
                     MLAGKTSIIHGFKNQAGVQALRVSPHSLAIAIAAKLNRPLRS"
     gene            complement(2277..4568)
                     /locus_tag="VFQ35_12295"
     CDS             complement(2277..4568)
                     /locus_tag="VFQ35_12295"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HET9931468.1"
                     /translation="MNKWWSWCALGAGFLVVVGCVAQGARPGPLPRGTKSEFRASAPA
                     GVAPAPVENVTSSSFAVPAAGIVAPLPGSPRAEELARVALQPSTCAPTAEAIRERALP
                     LIQQMRREVDEEYQRYQENLPNCWEEYRRNEEYWRSLKSGEQYGYGGLGLVGIGEGGG
                     GYGVGIGLGSVGTIGHGAAYGQAASASSVRAAKSHSKTNNQLADVDEADLVKTDGRYL
                     YVTLNGALRIVEALRPRVLSVTQLPGRVKDMFVEGDRAVVYSSIGGSGARSCKYAYDC
                     VFQGDGSSTRITVLDIRDRSAPKALRAIDFSGSVLASRRIGSAVHTVVSEGELAPPPY
                     ETWMSDLPECGVRDSAVRARVQRLKERNERAIREYVKTWAPSVRDGGVTRALCDDVYK
                     SQFDDGEVFTTLVSFDLERDAAPPVIASLRSRPGAVFASSDALYVSVAHTSVRDRKRG
                     YRPFGDSVREISEVHKFHIGSKPEDTRYVGSGVVPGHVLNQFSMDQWYGYLRIATSHG
                     RVPDSNVESTVSVLADDGKGGLVRVGAIEHIARGEDIRAVRFDGDRGYVVTFKKTDPL
                     FSLDLADPARPRLVGALKIPGFSTYLQRIDADHLISIGFDANDHGSFAYYDGLLLQLF
                     DVHAPTDPKLLFREKIGTRGSASEAATDHLAFTFFGERGLLAIPATICGGGGDGRNGD
                     TVTFSGLLVFQVDTKSGFKRLGGVDHGAPGGDCSSWWSNSSSAVKRSVFLDDLVYSIA
                     NDRVKVQNLARLGTDIADLDMQK"
     gene            complement(5243..>5799)
                     /locus_tag="VFQ35_12300"
     CDS             complement(5243..>5799)
                     /locus_tag="VFQ35_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9931469.1"
                     /translation="AGGARGVQAPGRAPRATQGQVMATSDVATVTVVVRAEVGLAFQV
                     FSEEIDTWWRTGPRFRIGRRAVGRLTFEAGVGGRLFETYETAAGTKTHVSGNVTVWEP
                     PRRLAFEWRGVNFRPDESTLVEVEFEPLGDNTRVTLRHSGWSRLPDDHPVRHGLAGAT
                     FIREIGMWWAALLSSLREHVGTRA"
BASE COUNT         1070 a         1945 c         1806 g          978 t
ORIGIN      
        1 gacttcgagc acgaggactc gtacagcgtg agtctgggcg cgagccctct cgtgaacccg
       61 aagtcaagcg tcgcggcgac gctcggcacc gcacaaccgc tctcgggcat gcaagagctg
      121 cgggtcgact tcgagtatcg aaacgagagc accgcgtgga cgcagtggat gtactttccg
      181 ctgcgcttcg atcgcactcg cgacttcaaa tcgctcgccg gcgtccgctt tctcgcgcgc
      241 gccgactcag cgcggaccct gcgcgtcgat ctcgagagca tcgtttatca agccggcaac
      301 gagggtatca agttcgggtg ggaggtcccc gttggccctg aagcgaccgc ggtcgaggtc
      361 cgcttcgccg acgccaagct gccgagctgg gcgcatgcca cgacagacgt actcgccgct
      421 gtcctcgcca agctcgatgg gctctcgttt catccgttct gcgccggccg cgaccaaagc
      481 gggcagctac cggatggaac ctccgacgca ggacacctcg agctcgacga catcgagttc
      541 ttctgaccag ggcgcggtcg tctacggcgg cggaagcgcg agctcgccag ggtagacgac
      601 cacttcgagc cgccgcgcga agtgcaggtg cggcggtggt ccgctcaccg cgataccgtg
      661 aaaatcgagc atggtgttcg tttgcagccg gagctcggcg cgctgcaacg gccacggcac
      721 gtgatgcact tcgttgcgga cgagcgcgcc attcggagaa agcgcataca aacaatagcg
      781 ctcggtgaga aagtgctcga gcgtgccggg ctcgctgaga taggtttccg aagtcggacg
      841 atagctcccg aggaactctg ctcccgctcg tttcgacccg tagtggatga actcctgctc
      901 gacgcggacc gacatcgcgg cgcgataata cggcaggaaa aagaaccggc gcgccgccca
      961 gacggccaaa ggattcgtcg cgtccaagct caagaaccag actccgggct tgccgtcgcg
     1021 ctcgacgtaa aggcgcacgt tgagctcggg aaacgcggac acccacggca tgttgggcag
     1081 cgggcggcgc atcacgcctt ccatccggaa cggtacgacg ccgacccaag atgtgccgtc
     1141 gaactcctga atttcgaggt cgggaggaac cagcggccga agcaccgata ccggcacggg
     1201 ccaatgagcg aacagcaagt cgcaccaagt ttgccgccag ctccatttgg tcgtggggag
     1261 cggccacggt ctgtgcgcgg ttcgttcgaa cgccggatgc gccatcagcg ccgagcgtag
     1321 tgaagcgtca gcgagacgca atcgtcctcg cgctacggca tgatcgccgc ctcgtgtcga
     1381 ggcggttcgt ctgcggaccg tcgcgccgag tcgcgcggct cagctccgga gcggccggtt
     1441 cagcttcgcg gcgatggcga tcgcgagcga gtgtgggctc acccggagcg cctgcacgcc
     1501 cgcttggttt ttgaacccgt gaatgatgct ggtcttgcct gcgagcatcg cgcggtacgc
     1561 ctcgcgcgct acatcgatcg gttccgcgac cgcggcgcga aagggcggag tgttgtcggt
     1621 gccggcgctg ctgctgaact gagtcgcggt ggcgcccggg cagctcagag tcaccgtcac
     1681 tcccgtccct ttgagttcga acgcgagcgc ctcgctgaac gagttcacga acgccttcgt
     1741 cgcgtagtag gtcgccatga acggccccgg ctgaaaaccc gaggtcgacc cgatgttcaa
     1801 gatgcgtccc ccaccgcggc tgaacatgga cggcaggaac aggtacgtga gctccacgag
     1861 cgccttgacg ttgacgtcca ccatttggag ctctcgctcc acgtcttgct ccacgaacgc
     1921 gccgttcgac ccgaaccctg cgtcgttcac cagaaactcg acgtcgaggc tctgcgcgcg
     1981 gaccgcttcg aagatcgccg agggactctc gcgctggccg aggtcgcgag cgatgacgat
     2041 ggctggcact ccgcgcaccg ccgagagctc acgggctagc gcgtcgagct cgtccttgcg
     2101 ccgcgcgacg agcacgacac cgtgaccatc tgccgcgaag agcttcgcca gctccttgcc
     2161 aatgcccgaa gaggcgcccg tcactacggc aaacgtcgtc atcgcgcgag gctacacccg
     2221 ttgtagctcc gaagggccac cggattggca gatcgcgttc cgccaactga cattgctcat
     2281 ttttgcatgt cgaggtccgc aatatcggtc ccgaggcgcg ccaaattctg aactttcact
     2341 cggtcgttcg cgatcgagta aacgaggtcg tccagaaaga cgctgcgctt gaccgcggaa
     2401 gaagagttcg accaccagga agagcagtcc ccaccgggcg cgccgtggtc gactccaccg
     2461 agccgcttga agccgctctt cgtgtcgacc tggaagacga gtagcccgct gaacgtcacc
     2521 gtatcgccat tgcgaccgtc gccgcctcca ccgcaaatcg tcgcggggat ggcgagcaac
     2581 ccgcgctcgc cgaaaaaggt gaatgccagg tgatccgtcg ccgcttccga agccgagccg
     2641 cgcgtgccga tcttctcgcg gaagagcagc ttgggatcgg ttggggcgtg aacgtcgaag
     2701 agctgaagta agagcccgtc gtagtaagcg aagctaccgt gatcgttcgc gtcgaagccg
     2761 atcgagatca agtgatcggc atcgatgcgc tggaggtacg tcgagaatcc tggaatcttg
     2821 agcgcgccga ccaagcgcgg gcgagccgga tcggctagat cgagcgagaa caaagggtcc
     2881 gtcttcttga aggtgacgac gtaaccgcga tcgccgtcga agcgcaccgc gcgaatgtcc
     2941 tcgccccgag cgatgtgctc gatcgcgccc acacggacga gcccaccctt tccgtcgtcg
     3001 gcgagcacgg agacggtgct ctcgacgttc gaatccggaa cgcgaccgtg cgacgtcgcg
     3061 atccgcaagt agccgtacca ttggtccatc gagaattggt tcaagacgtg tccgggcacg
     3121 actccgctgc ccacgtaacg agtgtcctcg ggcttgctgc cgatgtggaa cttgtggacc
     3181 tcgctgattt cacgaacgga gtcaccgaag gggcggtaac cgcgcttgcg atcgcgcacg
     3241 ctcgtatgag cgaccgaaac gtagagcgcg tcgctggagg cgaacacggc tccggggcgg
     3301 cttcgcagcg aagcaatgac gggtggcgcg gcgtcccgct ccaggtcgaa cgaaaccaac
     3361 gtcgtgaaca cttcgccgtc gtcgaactgg ctcttgtaga cgtcgtcgca gagagctcgc
     3421 gtgacgcccc cgtcccgcac cgaaggcgcc caggtcttta cgtactcgcg aatcgcgcgt
     3481 tcgttgcgct ccttcaggcg ctgaacccgt gcgcgcaccg ccgaatcgcg cacgccgcat
     3541 tcgggcaggt cgctcatcca ggtttcgtat ggcggcggcg cgagctcgcc ttcgctcacg
     3601 acagtgtgca cggcgctccc gattcgtcgc gaagcgagca ccgagccgga gaaatcgatc
     3661 gcgcgcagcg ccttcggggc ggagcgatct cgaatgtcga gcacggtgat gcgcgtcgag
     3721 ctgccatcgc cttggaacac gcagtcgtag gcgtatttgc aggatcgtgc gccgctccct
     3781 ccgatactgg aatatacgac cgcgcggtcg ccttcgacga acatgtcctt cactctgccg
     3841 ggcagctgcg tgacggagag cacccgcgga cgcaacgctt ccacgatgcg caaagcaccg
     3901 ttcagcgtga cgtagagata gcgaccgtcc gtctttacca ggtcggcctc gtcgacgtca
     3961 gcgagctggt tgttcgtttt ggaatggctc ttcgccgcac gcaccgacga cgcgctggcg
     4021 gcctggccat aagccgcgcc atggccaatc gttccgacac tgccgaggcc gatgccgacc
     4081 ccgtagccac cgcccccctc gccgatgccg acgagcccga gcccaccgta cccgtactgc
     4141 tcgccgctct tcaggctccg ccagtactct tcgttccgtc ggtactcctc ccagcagttc
     4201 ggcaggttct cctggtaacg ctggtactcc tcgtcgacct cgcggcgcat ctgctgaatc
     4261 agcggaagcg cgcgctcgcg aatggcctct gcggtcggtg cacaggtgct gggctgcaag
     4321 gcgactcgag ccaactcttc tgctcgcggt gatccgggca aaggagccac gatgccggcg
     4381 gctggcaccg caaacgagct ggacgtgacg ttttcgaccg gcgccggcgc gaccccggct
     4441 ggcgcgctag cgcgaaactc cgacttcgtg ccgcgcggca acggtcccgg gcgcgctcct
     4501 tgcgcaacgc agccgacgac gaccagaaaa cccgcaccga gagcacacca cgaccaccac
     4561 ttgttcatga gggagcccgt tcggcagtcg cccaacgcgg ctaccgcacg gctgagacct
     4621 caatccgaag ccgaagttcc ccaatcggga ggaagggcga ttcggggaac tatttcgagc
     4681 ccgagctgtc gaaacgaccg cggcccgcgc tgggtcttca cctcacgcaa tcgcggtccg
     4741 aagcccgcgc ctacatgccc aagcagtacc gctgcacgtg gctgcgctct gggtcgaacc
     4801 cacacgaggc acccgcgcaa cagtccgcgt tcgaatagca gggtcggtcc tcgcaggtgt
     4861 tcgcggtggc gttcgcggcc gcgtcgtcga acgccgagcc tcgcgccgcg cgtggagcgc
     4921 tgggctccgc attcgagcca ctgacgttgg gttctttggg gggcgcgctc gcgcacgaaa
     4981 gaacggttcc gacagccaat acactggcaa acgaaacgac cattcgcgat atcattcggt
     5041 cctcctcggc gacaaagtcg ctctggtcag agagacacga caggccgact gccgctccgg
     5101 cggcttcggc agttagtagt gtactcctcg cgcgcggcgc ggcacgcctt ctaggatcca
     5161 atcgaagtgc gaaacgtcag tcgatcacgt ttttgccagt acgctcgatc gccccactca
     5221 cgaatgcgct cggccaaccg cgtcaggctc gggtcccgac atgctcccga agcgagctca
     5281 agagcgccgc ccaccacatg ccaatttcgc gaatgaaggt cgctcctgcc agaccatgac
     5341 gcaccggatg atcatcgggc aggcgactcc acccggaatg acgcagcgtg acgcgcgtat
     5401 tgtcgccgag tggctcgaac tcgacttcga ccaaggtgct ctcgtctggc cgaaagttga
     5461 cgcccctcca ctcgaacgcg agacggcgag gaggctccca gaccgttacg ttcccagaca
     5521 cgtgcgtctt cgttcccgca gccgtctcgt acgtttcgaa caatcgtccg cccacgcccg
     5581 cctcgaacgt gaggcgcccg accgcgcgac gcccgattcg aaatcgcgga ccggtacgcc
     5641 accaagtgtc tatctcttcg ctgaacacct ggaacgccaa acccacttcg gcgcgaacca
     5701 cgaccgtaac ggttgcgacg tcggaagtag ccatcacttg cccttgcgtc gctcgcggag
     5761 ctcgacctgg cgcttgaacg ccgcgagctc cgcctgcca
//
LOCUS       DASTKS010000595         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_96535,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000595 DASTKS010000000
VERSION     DASTKS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96535"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1138)
                     /locus_tag="VFQ35_12305"
     CDS             complement(173..1138)
                     /locus_tag="VFQ35_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET9931470.1"
                     /translation="MTSSGDVSDRLEQTIARFVRHLADERRMSPHTVDAYGRDLRALA
                     AFVREKLGTAAALERIDRPLLRAYLGALAESRSPVTISRKLASLRAFFGYLERHDGLR
                     KNPAALLASPRLRRKLPKFLNTDAAAGVMQAPLASNGAQGPEHVRDTAMLELMYGSGL
                     RVSELVGLDVEHVALAARELRVLGKGRKERVVPLGSKARAALEQWFVTRPELLNGAPD
                     KGAVFLSRRGRRISVRWVQRLVRRYGILGAGRPDLHPHALRHSCATHMLEGGADLRAI
                     QELLGHSSLTTTQRYTHVSMDQLLSVYDRAHPLARAKKRRNSGSP"
     gene            complement(1148..1720)
                     /locus_tag="VFQ35_12310"
     CDS             complement(1148..1720)
                     /locus_tag="VFQ35_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931471.1"
                     /translation="MSLRTMVAGRAALAASERAFDQGELRESVRLAGRAATLAVPGAD
                     HVELAYRRLVIIARGAEAAGRPHLAAFAWNTVRGAAVESAAPGFGVRPELELANQNLA
                     RLASRLATDKDDPASEREISKTLERPPGPSAEGLWLLAGGLVLLVAGLGCVGLLGFNR
                     DGRAQPAKLGWGLLVVLLGAACWTLMAYRA"
     gene            complement(1801..>2496)
                     /locus_tag="VFQ35_12315"
     CDS             complement(1801..>2496)
                     /locus_tag="VFQ35_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931472.1"
                     /translation="ELKTIFPILARPIRRPEYLVGKYLGTLLTLGVFVAANTGALLLS
                     LAELSGRSLALCVLWAFGAALVCAAIGWRLPRTRTWLPIPFSLVLFASGVVLAAGAPD
                     DRGVLVGSALLTLCEVGIVTAVATVFAAFSSPFLTAVFTFGVFVVGRSADTLANLPVR
                     MFGETLRAIGQTLGHVVPNLMLYVPERPLLTGEAEGASLLGYLGLAAAYAAAWVLGLL
                     TFASLVFRRRDFL"
BASE COUNT          480 a          854 c          839 g          323 t
ORIGIN      
        1 gggaccaccg gagcgagaag cccgagcgag gtgagccagg cgctcgacaa cgacgtccgt
       61 gatgcgagcg ccacggcgcg cgcgtcgagg aacgcggcgg ctacgcttcc gaggcaagcg
      121 ccgagcaacg cgcccagcac ccgtgccgag agagagctcg cggccgtcga cttcatgggc
      181 tgccggagtt acgccgcttt ttggcccgtg ccaaggggtg agcgcggtcg taaaccgaaa
      241 gcagctgatc catggacacg tgcgtgtagc gctgggtggt agtcaggctc gagtgtccca
      301 aaagctcctg aatggctcgc aaatcggccc cgccctcgag catgtgcgtc gcgcacgagt
      361 gccgaagcgc atggggatgc aaatcgggcc ggcccgcgcc caagatgccg tagcggcgca
      421 ccagtctttg cacccagcgc acgctgatgc gacgtccgcg ccggctcagg aacaccgcgc
      481 ctttgtcggg cgctccgttc aacagctcgg gccgtgtgac aaaccattgc tcgagagcgg
      541 cgcgcgcctt cgagccgagc ggcaccaccc gttccttacg accctttccg agcaccctga
      601 gctcgcgcgc ggcgagcgca acatgctcga cgtcgagccc aacgagttcg ctcacgcgta
      661 gccccgagcc gtacatgagc tcgagcatcg cagtgtctcg aacgtgctct ggtccctgcg
      721 ctccgttcga cgcgagcggc gcctgcatca cacccgccgc ggcgtcggtg ttcaaaaact
      781 tcgggagctt gcggcgaagc ctgggtgagg ccaggagcgc agccggattt ttgcgcagac
      841 catcgtggcg ctcgaggtag ccgaagaatg cgcgcaggct cgcgagcttc cgagagatgg
      901 tgacgggcga ccgcgattcg gcgagcgcgc cgaggtaggc gcgcaaaagc ggccggtcga
      961 tgcgctcgag ggcagccgcc gtgccgagct tttcgcgaac gaacgcggcg agagcacgta
     1021 ggtcgcgccc gtaggcgtcc acggtgtggg gtgacatgcg ccgctcgtcg gcgaggtgac
     1081 gcacgaagcg agcgatggtt tgctccagcc ggtcgctgac gtcacccgaa gaggtcaccc
     1141 tggagtttta cgcccgatag gccatgaggg tccaacaagc ggcgccgagg agcacgacca
     1201 gaagccccca gccgagcttc gccggttggg cgcgcccgtc tcggttgaaa ccgagcagac
     1261 cgacgcagcc taggcctgcg acgagcaaaa ccaagccgcc tgccaagagc cagaggccct
     1321 ccgcgcttgg gcccggggga cgctcgagcg tcttcgagat ttcgcgctcg ctcgccgggt
     1381 cgtccttatc ggtcgcgagt cgactcgcga gcctcgcgag gttctgattg gcgagctcga
     1441 gctccggacg gactccgaag cctggcgccg cgctctcgac cgccgccccg cgcacggtat
     1501 tccaagcgaa cgccgcgaga tgcggccgtc ccgcagcttc ggcgccgcga gcgatgatga
     1561 cgagccggcg ataagcgagt tcgacgtgat ccgcgccggg cacggctagg gttgcagcgc
     1621 gcccagccaa tcgaaccgat tcccgcagct cgccctggtc gaacgcccgc tcggaagccg
     1681 cgagtgcagc acgcccagcg accatggtgc gaagcgacat gacagccagc gctcccagca
     1741 agatcgccgt tgccaatgca agccagcgcg cggagcgagc caaccgagcc gagagcctgc
     1801 tcataggaag tcgcgccgcc ggaacacgag gctcgcgaac gtcagtaagc cgagcaccca
     1861 agccgccgcg tacgccgcgg cgagacccag atatccgagg agcgacgcgc cctcggcctc
     1921 tcccgtcagc aggggacgct cgggcacgta caacatcagg ttgggcacca cgtgacccag
     1981 cgtctgcccg attgcgcgca acgtctcgcc aaacatgcgc accggcaggt ttgccaacgt
     2041 atcagccgag cgcccgacga cgaagacgcc gaacgtgaac acggccgtga ggaacggcga
     2101 tgagaacgca gcgaacacgg tcgccaccgc cgtgacgata ccgacttcgc acagcgtgag
     2161 gagggccgag ccgaccagca ctccccggtc gtcgggtgcg cccgcggcga gcacgacccc
     2221 cgacgcgaac agcacgagcg agaagggaat cggcagccaa gtgcgcgtgc ggggaaggcg
     2281 ccaaccgatc gccgcgcaca cgagcgctgc cccgaaagcc catagaacgc agagcgcgag
     2341 gctacgcccc gaaagctctg cgagcgaaag caacagcgct cccgtgttcg cggcaacgaa
     2401 aacgcccagg gtgagcagcg tgcccaggta cttgccaacc aggtactcgg gacgccgaat
     2461 cggccgcgcc aggatcggga aaatcgtctt gagctc
//
LOCUS       DASTKS010000596         5388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1435745, whole genome shotgun sequence.
ACCESSION   DASTKS010000596 DASTKS010000000
VERSION     DASTKS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435745"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFQ35_12320"
     CDS             <1..666
                     /locus_tag="VFQ35_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931473.1"
                     /translation="QAKLLRVLESRQVLPVGALVPQPIDVRFVAATNRDLRAEVARGA
                     FREDLFFRLAGVKIAIPPLRERPSEIAPLTDEIALAMTTALGRTAPSFATCARECLER
                     YAWPGNVRELRNVVESAVLRSEGGLVRAEDLPEELQFQQKRADASGRHRVRESDEFTS
                     SARAGVPPEQLTSRQREERARILDGLAACNGNQTRAAALLGMPRRTLVYKLAAYAIPR
                     PRI"
     gene            complement(900..1355)
                     /locus_tag="VFQ35_12325"
     CDS             complement(900..1355)
                     /locus_tag="VFQ35_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931474.1"
                     /translation="MLLTPPLATELPPPELMPPAPEPFDDPPSPVAPPRAADPPAAGP
                     EPPLPFEAPPALELVPPELDLAPPALDDVVPPVPIGPEPELLAEPPELETFPPLPGSP
                     PDEGLLQAASQGATAAAQSSGAPHLLRIKRLDDEVKTNCTALRESLDKG"
     gene            1345..2628
                     /locus_tag="VFQ35_12330"
     CDS             1345..2628
                     /locus_tag="VFQ35_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931475.1"
                     /translation="MSSTNHDHCLFGYDPEPSDNTMASGPLPYKLSDGTYDTVFQQPV
                     QDWMEANKWTGAHVIWHAVRGCKDGTAAGLLGPLGLPNLCKDYPVLVPTDQNCKSDGD
                     GYQFLLFHRHMLQALKQLWPKHTADFDGFPKFPTKKEELPNVWNDKDPTWNKQILDAA
                     AIGDSIEQHLDLFPDEGALGYWLQCPVGSPKQTFAPNIPYVGLHFNMHDQWSRGASSP
                     HGLNNGQANVTNYMFWKLHGWIDKVWEKYRVAKGLTKEGSPEMQKYKQDLAQACHEMD
                     TEIQILKSNNGQAVLDCPPDVDETGDFHTKVRPIFENATNHCASCHGPSQSSPYANLT
                     LGGAVSSKCIVQRLKQASLDGGQYKLVEPGNPDKSWLYLKAAGKAADANCMSTDPNRP
                     CNTATMPPNGTGVPTMTAAELEILRKWIADGANGP"
     gene            complement(2796..4865)
                     /locus_tag="VFQ35_12335"
     CDS             complement(2796..4865)
                     /locus_tag="VFQ35_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931476.1"
                     /translation="MTSTPKALLSLTALRNLLLLSLLIGVAAYTATLFAYSQRLKNTF
                     GPQVRADLEWRVLRGAQELARACDVGLVLGDPAMLKKSFGAYATSADVQAIVAVDAEG
                     KVLTTHGKSPEAIDKLFAGKELGMRVEPGYLVSWADATIEGSKVGRIAVVVSTARLSD
                     ANALLARASNVTLLGGTLALVLGVLVVSSFTRAVARRDAQLSEHAANLEQKVAERTQE
                     LDERNRGMRVVLDNVGQGLVTVDLAGVMANERSASVDRWFGPPTRSMTLSQYLKQHSP
                     AFAQQFELNMMQLADGFLAPELALDQLPKRMLAGNRTFDVHYTPVGAEEQFDKLLVIF
                     DDVTEQLASERAEREQKEAVAIFQRILVDRSGVEDFLNEANKFLSELHTQRDPIVERR
                     LLHTLKGNAAVFGLGSLADQAHELETQLHERDVKTGDHMALLEPAELEPFDHAWREAM
                     ARVQQLLGGARRDQIEIGQGELTSLVDRVRAGLPASEIAHELEAWKQEPVERRLEQLG
                     RQASTLARRLGKAAPALVIESSGVRSDGRSWAGYWSAMVHAVRNAVDHGLEDSAARVA
                     AGKPAAGRIALKAERSAKRLIISVEDDGRGIDWEKVRTKAASCGAPHGTHDELVDALF
                     LDGFSTREQVSDISGRGVGLAALRQAVRELGGSIEVDTNLGRGTAFRFIFDEPKAQRP
                     SIRAVAS"
BASE COUNT          928 a         1739 c         1807 g          914 t
ORIGIN      
        1 caggcaaagc tgctgcgggt gctcgagtcg aggcaggtgt tgccggtggg cgcactcgtg
       61 cctcagccca tcgacgtgcg cttcgttgcc gccaccaatc gcgatctgcg agccgaggtg
      121 gcgcgcggcg cattcaggga agatctgttc tttcggttgg cgggggtgaa aatcgcgatc
      181 cctccgttgc gcgagcgacc cagtgagatc gccccgctca ccgacgaaat cgccctcgcc
      241 atgacgacgg cgctcggccg gaccgcgccg agctttgcga cctgcgctcg agagtgcctc
      301 gagcgatacg cttggcccgg caatgtccgc gagcttcgca acgtggtcga gagcgcggtg
      361 ctccgcagtg agggagggct ggtccgtgcc gaggacttgc ccgaagagct gcagttccag
      421 caaaagcgag ccgacgccag cgggcgtcac cgcgtcagag agagcgacga gttcacgtcg
      481 agcgcacgcg ctggtgtgcc gcccgagcaa ttgaccagcc gtcaacgcga ggagcgcgcg
      541 cggatcctcg acggcctcgc tgcgtgcaac ggtaaccaga cgcgggcagc ggcgctgctc
      601 ggcatgccga ggagaacgct ggtttacaag ctcgccgcct acgccatccc gcgtcctcgc
      661 atctgacttc acgcgcagtc tgaggccagc gcgacgaatt cgtcgcgaac tcgtcaccca
      721 ttcgaaacgg gacgctaggg ccaacaatcc gtggcgcaac ctcgccgtgt tcggcgacag
      781 aagtatcgtt gctcgaagtg aacggatgcg ctcgccatga aaaatttcca gccgcgctcg
      841 gcatcttgag gttttccaaa tacgaagcgc agtgagcggg cacctattgc tggagtcgtc
      901 taccccttgt ccagcgactc acggagcgcc gtgcaattcg tttttacctc gtcatccaag
      961 cgttttatcc ggagcaagtg gggcgcaccg ctgctctgcg cagctgctgt ggcaccttgg
     1021 ctagcggctt gtagcagccc ttcgtcgggc ggggatcccg ggagtggagg gaaggtttcg
     1081 agttccggcg gctcggccaa tagctctggc tcgggtccga tgggaaccgg cgggacgacg
     1141 tcgtcgagtg caggtggtgc caagtcgagc tctgggggaa cgagctcgag cgctgggggc
     1201 gcttcgaacg gaagtggtgg ttccgggcca gcggcggggg gatccgctgc gcgcgggggc
     1261 gccaccggcg acggcggatc gtcgaacggc tcgggtgcgg gcggcatgag ctcgggcggt
     1321 ggcagctccg ttgccagcgg cggagtgagc agcacgaacc acgatcactg cctgttcggc
     1381 tacgatccgg agccgagcga caacacgatg gcgagcggcc cgctccctta caagctgtcg
     1441 gatggcacgt acgacaccgt ctttcagcaa cccgtccagg actggatgga ggcgaacaag
     1501 tggactggcg ctcacgtgat ctggcacgcc gttcgcggct gcaaggacgg cacggcggct
     1561 ggccttttgg gcccgctggg cctgccgaac ctctgcaaag actaccccgt cttggttccc
     1621 accgaccaaa actgcaaatc cgacggcgac gggtatcagt tccttctgtt ccatcgccac
     1681 atgctgcagg cgctgaagca actgtggccc aagcacacgg ccgacttcga cggtttcccg
     1741 aagttcccca ccaagaagga agagctgccc aacgtctgga acgacaaaga cccgacgtgg
     1801 aacaagcaga tcctggatgc cgccgcgatt ggcgacagca tcgagcagca tttggatctc
     1861 ttcccggatg aaggcgcgct cggttactgg ctgcagtgtc cggtgggctc acccaagcaa
     1921 acgttcgcgc cgaacattcc gtacgtcggc ttgcacttca acatgcacga tcagtggtcg
     1981 cgcggtgcga gcagcccgca cggcctcaac aacggccagg cgaacgtcac gaactacatg
     2041 ttctggaagc tgcacggctg gatcgacaag gtctgggaaa agtaccgcgt cgccaagggc
     2101 ctcaccaaag agggctctcc ggagatgcag aagtacaagc aggatctcgc gcaggcctgc
     2161 cacgaaatgg acaccgagat tcagatcctg aagtcgaaca acggtcaagc ggtgctcgac
     2221 tgcccacccg acgtcgacga gacgggcgat ttccacacca aggtgcgtcc gatcttcgag
     2281 aacgcgacca accactgcgc gagctgtcac ggcccaagtc agtcctctcc ttacgcgaac
     2341 ctgacgttgg gcggagccgt ttcgagcaag tgcatcgtcc agcgcctcaa gcaggcgtca
     2401 ctcgacggcg ggcagtacaa gctggtcgag ccgggcaacc ccgacaagag ctggctctac
     2461 ctcaaggcgg cgggcaaagc cgcggacgcc aactgcatgt cgacggatcc gaaccgcccg
     2521 tgcaacacgg cgaccatgcc accgaacggt actggcgttc ccacgatgac ggcagccgag
     2581 ctcgagatcc tgcgaaagtg gatcgccgac ggcgccaacg gtccatgagc cgtgacggag
     2641 ctcgacggtt cttgccgtcg agctccgcgc ccgaaccaaa caaaggcgtt ggacgagccg
     2701 ctgggcaact ggcggctcgc gctgcttcgt gggcccatcg gcgtcgatgc gacgctcgag
     2761 gaggccgcga cgaacgcggg accactgcag gtcgactacg aagcgacggc tcggatcgac
     2821 ggccgctgcg ccttgggctc gtcgaaaatg aagcggaagg cggtgccgcg ccccaggttc
     2881 gtgtcgactt cgatgctgcc gccgagctcg cgtaccgctt ggcggagcgc agcgagtccc
     2941 acgccgcgtc cggagatgtc gctcacctgt tcgcgagtgc tgaagccgtc gaggaacagc
     3001 gcgtcgacga gctcgtcgtg tgtaccgtgg ggcgcgccac aactcgcggc tttggtgcgg
     3061 accttttccc aatcgatgcc gcgaccgtcg tcctcgacgg agatgatcag ccgcttggcg
     3121 gagcgctcgg ctttgagcgc gatgcggcca gcggcgggct tgcccgcggc gactcgcgcg
     3181 gcgctgtctt cgagcccgtg gtccaccgca ttgcgcaccg cgtgcaccat cgccgaccag
     3241 tagcccgccc aggagcgccc gtcggagcgt acgccgctcg attcgatgac gagggcgggg
     3301 gcggctttgc cgagtcgacg agccaaagta ctcgcctgac ggccgagctg ctcgagtcgg
     3361 cgttcgaccg gctcctgctt ccaggcttcg agttcgtggg cgatctcgct cgccggaagc
     3421 cctgcgcgga ctcgatcgac caacgaggtg agctcgccct gaccgatttc gatttggtct
     3481 ctgcgcgcac cgccgagcag ctgctggacg cgggccatcg cttcgcgcca cgcatggtcg
     3541 aacggctcta gctccgccgg ttcgagcagg gccatgtggt cgccggtctt gacgtcgcgc
     3601 tcgtggagct gggtttcgag ctcgtgcgct tggtcggcga gagagccgag cccgaacacg
     3661 gcggcgttgc ctttcagcgt gtgcagcagg cgacgctcga cgatggggtc gcgttgggta
     3721 tggagctcgc tcaggaactt gttcgcttcg ttgaggaagt cttccacgcc cgagcgatcg
     3781 accaggatgc gttggaagat cgccaccgct tccttctgct cgcgttcggc gcgttcgctc
     3841 gcgagttgct cggtgacgtc gtcgaagatc acgagcagct tgtcgaactg ctcctcggct
     3901 cccaccggcg tatagtgcac gtcgaaggtg cggttgccgg cgagcatgcg cttcgggagc
     3961 tggtcgagag cgagctcggg cgccaagaag ccgtccgcca gctgcatcat gttcagctcg
     4021 aattgctgcg cgaaggccgg cgaatgttgc ttcaggtact gactgagcgt catcgagcgc
     4081 gtgggcgggc cgaaccaacg atcgacgctg gcagagcgct cgttcgccat cactcccgcc
     4141 aagtcgaccg tgacgaggcc ctggccgacg ttgtcgagca cgacgcgcat gccacggttg
     4201 cgctcgtcga gctcttgcgt gcgctcggcc actttctgct ccaaattcgc ggcgtgctcg
     4261 gagagctgcg cgtcgcgccg cgccaccgcg cgcgtgaacg aagacacgac gaggaccccg
     4321 aggacgagcg cgagcgtacc accgaggagc gtgacgttgg aggcgcgcgc caagagcgcg
     4381 ttcgcgtccg agagccgcgc ggtcgagacc actaccgcga tgcgaccgac cttgcttccc
     4441 tcgatcgtgg cgtcggccca gctcacgagg taaccgggtt cgacgcgcat gccgagctct
     4501 ttgccggcga acagcttatc gatcgcctcg ggcgacttgc cgtgcgtggt cagcactttg
     4561 ccctcggcat ccacggccac gattgcctga acgtcagccg aggtcgcata cgcgccaaac
     4621 gacttcttca gcatcgccgg atcgccgagt accaagccca cgtcgcaagc gcgcgcgagc
     4681 tcttgagctc cgcgcaacac gcgccactcg agatctgctc ggacttgcgg accaaacgtg
     4741 tttttcaggc gctgcgaata ggcaaacaac gttgccgtgt aggcggcgac gccgatgagc
     4801 agcgagagca ggagcagatt gcgaagcgca gtcagcgaca ggagtgcctt gggagtgctg
     4861 gtcatgtgac tcggcgagcg ggggctggcg ttcgcctctg cgcagtcgag cggtcactcg
     4921 aggcaaccgg gctcgtcgta cgcaaagggc gaggctcacg agtacaacgg ctcgaattgc
     4981 ctttgctcta gggcgtgccc cccctagggc gataatctcg tggcgccgtc gccgcaccta
     5041 cgcgcacccc cggcgcggcg gttccaaatt gggacgatgc gctgcactag gctgtgctgc
     5101 gacggcgcgt cgtggcgcgc ttgcgagacg taccgcgttt gcgcgtgctc gccgcgcgtc
     5161 gcgcggtggc agagggagaa ttggatcctc ccgctttgcg tcgccgctcg gcggaagttc
     5221 gcgcctggcg agatagcgcc gtgcgcgacg cggccgctcg cggctctttt tcgagagcgc
     5281 gctcggtcgc acgcgccctt cgagggtttg cgggcttgcc ggcacgagcg ctttcttgcg
     5341 cggccttgcg gcgtacgcgg ccgctcgcgt tcggccctgg cggagcgc
//
LOCUS       DASTKS010000597         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_987893, whole genome shotgun sequence.
ACCESSION   DASTKS010000597 DASTKS010000000
VERSION     DASTKS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987893"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1050
                     /locus_tag="VFQ35_12340"
     CDS             433..1050
                     /locus_tag="VFQ35_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9931477.1"
                     /translation="MAGGTFQRAHTPKPKDIPDLCFDVTEATAAEYDECVKAGACTKD
                     GVDCASQSTYGKADKAKLPMVCVEFSQAERYCAFRGKRLPTTDEWEWAARGGSEGRTY
                     PWGNDKPATQLCWAGKQKQLEACEVGSHPLDKSRDGLLDMAGNVLEFTTTENDAAAAV
                     RIARGGSWNSGAAELFRVSRLGAFGPSYRCGFLGIRCVEPASPAP"
     gene            1317..1805
                     /locus_tag="VFQ35_12345"
     CDS             1317..1805
                     /locus_tag="VFQ35_12345"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9931478.1"
                     /translation="MGDPPPPRAERGGVRYLVLEAISEQSRHGYEIIQHIEQRSRSTY
                     RPSPGVIYPTLQMLEELGHARVVEQEGRKAYSITELGKQDLEANRAAVDDFYSRCGED
                     PFESYADDFAELMKFFGRLMKTVKIGMHRGRMSPRTIRVIRQTLEEALKKIENALDER
                     DL"
     gene            1815..>2661
                     /locus_tag="VFQ35_12350"
     CDS             1815..>2661
                     /locus_tag="VFQ35_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931479.1"
                     /translation="MTALTKFDELPTYVVRAQREPVLERVEELELARRFREHGDRAAA
                     ERLTRAHLRVVVSIAHKYRRYGIPLADLIAEGNFGLAQALNKFEPERGLRFVTYASYW
                     IRAFVLDHVIRSWSMVGAGAGALRSRVFFRLRRERTRIANTLGTGEEADRVLAERLGL
                     GVDTVRNMMQRLDSRDVSLERESSAGESQLRLLDTLQSNDNQERDVLTREVEASVSSA
                     VGRAVAELDARERYIAERRLMADPSEELSLAEIGRELGVSRERARQLETRTKTKLKLR
                     IPALGG"
BASE COUNT          490 a          827 c          867 g          477 t
ORIGIN      
        1 ggtcgtgccc gccttggtcc ttcaaaccga ttggtcaggg gcggtaccca tggttgcgct
       61 gtacgacgcc gtccacgaac cggtgcgcac ggcgcgaggt gaacgtcgcg cgaaagacgc
      121 gcgcgaggcg gagacaatca cttggtagga gaaatcatgt cgttggtgag tcgtgcttca
      181 ttggtgcgcg tgttcgttct gggcgcgttc gcttgggctt gtgctgacac ccaagcaacc
      241 gaacgcgtcg cacaaagtcc gcccggggcc gctcctgcat ccagttcggt ggcttccgtg
      301 ccttccaatt cacccgcatc gctttcggca gggccgcgcg acgtaccttc cgagtctgct
      361 tcgccgccgg ccgcagccgc aagctcggct ccgactgcac ctgctcctcg atgcaaggcc
      421 aacgcggcgc tcgtggcagg aggcactttt cagcgtgcac atactccgaa gccgaaggac
      481 atcccagatc tttgcttcga cgtcaccgag gccaccgccg cggaatacga cgagtgcgtg
      541 aaggccggag cctgcactaa ggacggcgtc gactgcgcgt cgcagtcgac gtacggaaag
      601 gcagacaagg cgaagctccc gatggtgtgc gtcgagttct cccaggccga gcgctactgc
      661 gccttccgcg gaaagcgctt gcccaccacg gatgagtggg agtgggcggc gcgcggtggc
      721 tccgaggggc gcacgtaccc gtggggaaac gacaagcccg caactcagct ctgctgggca
      781 ggcaagcaga agcagctcga ggcgtgcgaa gtcgggagcc atccgctcga caagagccga
      841 gatgggctac tcgatatggc cggcaacgtg ctggagttca cgaccacgga gaacgacgcg
      901 gcggccgcgg tgcgcatcgc tcgcggtggg agctggaatt ccggtgcggc ggagctgttt
      961 cgcgtctcgc gcctcggtgc gttcgggccg agttaccgct gcggcttcct cggcattcgc
     1021 tgcgtggagc cggcgtcgcc ggccccttag gctctaaaag ctcacacctc gtcggcctga
     1081 cccgccgatc ggccatttgg ccgatcttcg tgtacgcctg ttgacgttcg atgagatata
     1141 tctatagcaa tatcgacaga catatcggaa acccattccg aatcgtaact gtctgaaagc
     1201 tatacaaaat gcgtcacagt cacttctctc atgaaggctc atggtgggcc ggtcgctttg
     1261 gtcacgggtc cgggttcgga ccgggtcgtc gcggaggtcg tccctggtgg gccgacatgg
     1321 gtgacccgcc gccgccgcgc gccgagcgcg gaggggttcg ctacttggtg ctcgaagcga
     1381 tcagcgagca gtcccggcac ggctacgaaa tcattcaaca catcgagcag cgctcacgct
     1441 cgacgtatcg cccgagcccg ggtgtcatct atcccacgct acagatgctc gaggagctcg
     1501 ggcatgcccg cgtagtggag caagaaggtc gtaaagccta ttcgatcacc gagctcggga
     1561 agcaagatct cgaagcgaat cgcgccgcgg tagacgactt ctattcgcgc tgcggagaag
     1621 atcctttcga gtcgtacgcc gacgacttcg ccgagctcat gaagttcttc ggacgtctga
     1681 tgaagacggt gaagatcggc atgcaccgag gtcggatgtc gccgcggacc atccgcgtca
     1741 ttcgccaaac tctggaagaa gcgctcaaga agatcgagaa cgccctcgac gagcgagacc
     1801 tctgaggcct cagcatgaca gcgctgacca aattcgacga gctccccacg tatgtcgtcc
     1861 gagcccagcg cgagccggtc ctcgagcgcg tcgaggagct cgagctcgcg cgtcgcttta
     1921 gagagcacgg tgatcgcgcg gcggcagaac ggctcactcg cgcccatctg cgcgtcgtgg
     1981 tgagcatcgc tcacaaatac cgtcgctatg gcatccccct cgcggacctg atcgccgaag
     2041 gcaacttcgg gctcgcgcaa gcgttgaaca agttcgagcc ggagcgaggt ctgcgcttcg
     2101 tcacttacgc gagctactgg attcgcgcct tcgtgctcga tcacgtgatc cggtcgtgga
     2161 gcatggtcgg cgctggcgcg ggggccctgc gctcgcgcgt cttctttcgt ctacggcggg
     2221 agcgaacgcg tatcgcaaac acgctcggta ccggcgaaga ggcggaccgg gtgcttgcgg
     2281 agcggctcgg tctcggggtc gacacggtgc gcaacatgat gcaacggcta gactctcgcg
     2341 acgtttcgct cgaacgcgaa agttcagcgg gagaatcgca gctgaggctc ctcgacacgc
     2401 ttcagtcgaa cgacaatcag gagcgtgacg tcctcacgcg agaggtcgag gcttcagtca
     2461 gtagcgccgt gggccgtgcg gttgcagagc tcgacgcgcg cgagcgctac atcgccgagc
     2521 gtcgtttgat ggcagacccg agcgaagagc tttcgctcgc cgagatcggt cgagagctcg
     2581 gtgtttcgcg cgagcgcgct cggcagctcg agacgcggac gaagaccaag ctcaagctcc
     2641 gcattccggc tctcggtgga c
//
LOCUS       DASTKS010000598         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_96828,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000598 DASTKS010000000
VERSION     DASTKS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96828"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2532)
                     /gene="glnD"
                     /locus_tag="VFQ35_12355"
     CDS             complement(<1..>2532)
                     /gene="glnD"
                     /locus_tag="VFQ35_12355"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET9931480.1"
                     /translation="VMVRSSATPVTILQSPSLPAELKSYLANHRGQVEAKVRVADEGA
                     GLPAAERLAKAYDGLFCSLFKAVESTFSREGGWQSVGLAAVGSYGRGALSLHSDLDVR
                     LVTSSRPEKVRPIAEAMLYPLWDAGLSIGHQVVNPSDMLDLAKTDLPTATTLLDWRLV
                     AGERTSTEKMLTRAFEGLFNSANIAKVLERMAERAEERSERFGDSVYLLEPDVKNGIG
                     GLRDLDLAHWAARARWRVENLEELVRHGVLLPREWQQIDAAARFAWRVRNLLHLHAGR
                     RSDRLSFDLQEQIAGELGYGSTGPGVEQFMSEYYRHARVLWQSREMLLHRAEPPPKRR
                     PRESVIGRGLKLTNDQVSIENPVELETDPALALRLFDEAVRRDLPVYPFARDSVARAA
                     SSPTFCEKLRASEEAAKLFARLCTTVQRTRLRGGSVLKELHDVGLLVAMIPEFVPVVG
                     RVHHDVYHVYTVDVHSVAAVDRLRAFCRGELAAEHPLASRLAAEISRPNVLFFAALLH
                     DIGKDVGGRKHDERGAQMVGEILPRLHIPENDVREVQHLVAKHLRMYHIATRRDLDDP
                     KTLTAFVEELHGSEGLRELYLLTLCDVSTTSPTALTSWKARVLEELYRGAERQLDGDH
                     DHPDQRLDQIKTSVLSTCPERGEREFLQHFLAAMPERYLFANEPADIVRHSRFARQAQ
                     MRQVNVAVMTMAEPYVELGFIADDRPGLLAMITATLTAARFKVISSQVYSWVDSFGRT
                     RALDLFWVRGSTMDAVVSSTPRLERDFGRLMSQELSPADLVTGGSRTSRWSDRPTPAV
                     QTEVNVDNRCATRDTVIEVTTRDQLGLLFWLAHTLQSLGLTISLAK"
BASE COUNT          445 a          830 c          840 g          417 t
ORIGIN      
        1 ctttgcgagc gagatggtga gccccaaaga ttgcagcgta tgggccagcc agaacaagag
       61 cccgagctgg tcgcgcgtgg tgacctcgat gacggtgtcc cgcgtagcgc agcgattgtc
      121 cacgttgacc tccgtctgaa ccgccggcgt cggccgatcc gaccaacgtg aggtgcgcga
      181 acctccggtc acgagatctg ccgggctgag ctcctgactc atgagccggc cgaagtcacg
      241 ttcgaggcgc ggcgtcgaag acacgaccgc gtccatggtg ctgccgcgaa cccagaatag
      301 atcgagtgcc cgcgtgcggc cgaacgagtc gacccaagaa tagacctgcg agctgatgac
      361 cttgaaacgc gcagcggtga gcgtggccgt gatcatggcg agcaagccgg gccgatcgtc
      421 ggcgatgaag ccgagctcga cgtaaggctc ggccatcgtc atcacggcga cgttcacttg
      481 gcgcatctgc gcttggcgcg caaagcgtga gtggcgcacg atgtccgcgg gttcgttcgc
      541 gaacaagtag cgctccggca tggcggccag gaagtgctgc aagaactcac gctcgccgcg
      601 ctcggggcag gtcgagagca ccgacgtctt gatttggtcg agccgctgat ctgggtggtc
      661 gtgatccccg tcgagctgcc gctcggcgcc gcgatagagc tcttcgagca cgcgcgcctt
      721 ccagctcgtg agcgcggtcg ggctcgtcgt cgatacgtcg cacagcgtga gcaggtagag
      781 ctcgcgaagg ccttcgctgc cgtgcagctc ttcgacgaac gcggtcagcg tcttcgggtc
      841 gtcgaggtca cggcgggtgg cgatgtggta catgcgaagg tgcttcgcca cgaggtgctg
      901 tacctcgcgc acgtcgttct cggggatgtg caggcgcggc aagatctcgc cgaccatctg
      961 cgcgccgcgc tcgtcgtgtt tgcgaccacc gacgtccttg ccgatgtcat ggagaagcgc
     1021 ggcaaagaac agcacgttcg gccgcgaaat ttcggccgcg aggcgtgatg cgagcgggtg
     1081 ttcggcggcg agctcgccgc ggcagaaagc ccgcagccgg tccaccgcag cgaccgagtg
     1141 cacgtcgacc gtgtacacgt ggtagacgtc gtggtgcacg cgcccgacga ccggcacgaa
     1201 ctcggggatc atcgcgacga gcaagccgac gtcgtgcagc tctttcaaga cggagccacc
     1261 gcgcagccgc gtgcgctgca ccgtcgtaca caaacgagcg aagagcttcg ccgcttcctc
     1321 gctcgcgcga agcttttcgc agaacgtcgg cgaggacgcg gcgcgtgcca ccgagtcccg
     1381 cgcgaagggg tacaccggca aatcacgccg aaccgcctcg tcgaacaggc gaagggcgag
     1441 cgctggatcc gtctcgagct cgacgggatt ctcgatcgaa acctggtcgt tcgtgagctt
     1501 cagcccgcgt ccaatcacgc tctcgcgcgg acggcgcttc ggcggcggct cggcgcggtg
     1561 cagcagcatt tcgcggcttt gccagagcac ccgcgcgtgg cggtagtact cgctcatgaa
     1621 ctgctcgacg cccgggcccg tgctcccgta gccgagctca cccgcgatct gctcctgcaa
     1681 atcgaagctc aagcggtccg agcggcgccc cgcgtgcaag tggagcaagt ttcgaacacg
     1741 ccaagcgaag cgcgccgccg cgtcgatctg ctgccattca cgcggcaaaa gcacgccgtg
     1801 gcgcacgagc tcttccaggt tctcgacccg ccaacgggcg cgggccgccc agtgagcgag
     1861 atccaaatcg cgcagaccgc cgatgccgtt cttcacgtcg ggctcgagca gatacaccga
     1921 gtcgccgaag cgctccgaac gctcttcggc tcgctccgcc atgcgctcga ggaccttcgc
     1981 gatgtttgcg ctgttgaaga gtccttcgaa cgcgcgcgtc agcatcttct cggtgctggt
     2041 gcgctcgccg gccacgagcc gccaatcgag gagcgtggtc gcggtcggca agtcggtctt
     2101 cgcgagatcg agcatgtccg acggattcac gacttggtgt ccgatgctga gccccgcgtc
     2161 ccaaagcggg tagagcatcg cttcggcgat gggcctcacc ttctccggac gactcgacgt
     2221 cacgaggcgc acgtcgagat cgctgtgcaa ggacaaggcg ccgcgcccgt agctcccgac
     2281 cgcggcgagc cctacgcttt gccagccccc ttcacgagag aacgtcgact cgacggcttt
     2341 gaagagcgag cagaacaaac catcgtaagc tttggcgaga cgctcggccg ccggaagccc
     2401 agcgccctcg tcggcgacgc gcaccttcgc ttccacctgt ccccggtggt tggcgagata
     2461 gctcttgagc tcggccggta gggacggcga ttggagaatg gtgaccggag tcgcggaact
     2521 tcgaaccatg ac
//
LOCUS       DASTKS010000599         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_543473, whole genome shotgun sequence.
ACCESSION   DASTKS010000599 DASTKS010000000
VERSION     DASTKS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543473"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFQ35_12360"
     CDS             <1..798
                     /locus_tag="VFQ35_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9931481.1"
                     /translation="PKARFDVTIFDNVAPRLAENSGSALYLNPPSEGSPVKLEKPIKD
                     FGFDQWNRKSPLLRFLALGDVQVAHGNRLVPEKGDEVLAASDQGPILVSGARAGQHFV
                     VLAFDPRDSDFVLRPAWPLFVLGTIDAFVAEDTGYLSAYRTGQSWRIPAPSSAESAEL
                     TTPRGEKVLVPVKEGRAAYFGNQAGFYKLHVTGPEPFDHEFAANLADPEESQIKPQPK
                     LEVAGKAAPVAVAGRPGVRNRFWAYLLLGVVVVSVLEWFSYHRRLTV"
     gene            795..3551
                     /locus_tag="VFQ35_12365"
     CDS             795..3551
                     /locus_tag="VFQ35_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9931482.1"
                     /translation="MSPRARRLLFGVAVVASGLLAALLFYKCVWLAPEGAFRFVRDGR
                     TFELTSPSALAVLLVLPLLVFALGRSLADLPWPQRILSLFLRLSFLGLLALALASPVR
                     SESSTRICTVVLLDVSDSVTDESLVDARKEIERVLAARRPDDVVRLVTFAARPRVVEL
                     FKDDKPVIPEVSQLRHTDKQSNGGTSTNIQSALELAYGLFPPGYLKRELLLSDGVETD
                     GDLLSEANRARVFGVHLSATPFRRPPPSEISVVRLTLPQKVDIGQTFPVMAEIYASRE
                     NEARLRLFQGEALNGLDGVRDVKLHPGRNEVEFKSVVRVGGDVTYALELEPRGPDKFK
                     ENNAYRTTLEVPSRPLVLYVDGHPERASYLSNALSAQQFDVDLRGPSAFPGSLGELER
                     FDFVVLSDTPKEAFGAGSEELVERYVRDLGGGFLFAGGPAGYGLGGWAHSGIERLLPV
                     RMDAEHRKEIPGVAMALVIDRSGSMTGLNMEMAKAACSATVSTLQGDDLMEVIAFDAE
                     PQRYVKLQPVRYRTRIQGDIQRIQPGGGTEIFPALDMAYQDLSVVQARKKHVVLLTDG
                     QAPVQGIRELVQAMIAESITVTTVGLGPEANAELLRSIADSGGGRFHAVPDPNSLPKI
                     FTRETELVAQQAAVEEWFPVDQVGSADFLKGISISSAPLLHGYVSTELKPPPAQLILQ
                     SDRGEPILARWRAGLGWALAWTSDVKNGWAVEWLRWPNFGRFWGQLVREHMRVKRRRE
                     VDMKTEVDGSHVTATVDAFTPDERFDNEWQSKLIVRRVGSKGEPREVPMRQVAPGRYA
                     ADFDLDGFGSFLLRAEHARKERDGSLRPVGVSFAHVSNPYPREYASFEPDIERLTRAV
                     HAARGQIDPPPAKLFEPQGEQIIKHAELYSRFVLASLVAFLLDLLVRRVRLFDRKRLT
                     TRRA"
BASE COUNT          617 a         1236 c         1243 g          650 t
ORIGIN      
        1 ccgaaggcgc gcttcgacgt cacgatcttc gacaacgtcg cgccccgact cgcggagaac
       61 agcggttcgg cgctgtattt gaaccccccg agcgaaggca gcccggtgaa gctcgaaaag
      121 ccgatcaaag acttcggctt cgaccagtgg aaccgaaaga gcccgctctt gcgcttcctc
      181 gcgctcggcg acgtacaagt ggcgcacggc aaccgattgg tgcccgagaa gggcgacgaa
      241 gtgctcgcgg cgagcgacca gggtcccatc ttggtgagcg gcgcgcgcgc gggtcagcac
      301 ttcgtcgtgc tcgccttcga tccgcgcgac agtgacttcg tgctgcggcc ggcgtggccg
      361 ctgttcgtgc tcgggacgat cgatgcattc gtcgccgagg acaccggcta cttgtcggcg
      421 tatcgcaccg ggcagagctg gcgcatcccc gcaccgagca gcgccgaatc ggccgagctc
      481 accacgcctc gcggcgagaa ggtgctcgtc cccgtgaaag agggccgcgc cgcatatttc
      541 ggcaatcaag cggggtttta caagctccac gtgacggggc ccgagccctt cgaccacgag
      601 ttcgccgcaa acctcgccga cccggaagag agccagatca agccgcagcc caagctcgag
      661 gtcgcaggaa aggcggcccc ggtcgcggtc gcgggtaggc caggggtgcg caaccggttt
      721 tgggcgtacc tcttgctcgg cgtcgtggtc gtgtccgtgc tcgagtggtt cagctaccac
      781 cgtcggttga cagtatgagt ccgcgagctc gccggcttct gttcggtgtc gccgtcgtcg
      841 cgagcggcct cctcgcggcg ctgctctttt acaagtgcgt gtggctcgcg cccgaaggag
      901 cgttccgctt cgtgcgcgac gggcgaacct tcgagctgac ctcgccgagc gcgctcgcgg
      961 tgctcttggt gctgccgctg ctcgtattcg cgctcgggcg ctcgctcgcc gacctgccct
     1021 ggccgcagcg cattctgtcg ctgtttttgc ggctttcgtt cctggggctg ctcgcgctcg
     1081 cgcttgcgag tccggtgcgt tcggagtcgt cgacgcggat ttgcacggtg gtcctgctcg
     1141 acgtgagcga ctccgtcacc gacgagtcgc tcgtcgacgc acgcaaggaa atcgaacgag
     1201 tgctcgcagc gcgtcggccc gacgacgtcg tccggctcgt cacgttcgcg gctcggccgc
     1261 gcgtggtcga gctgttcaaa gacgacaagc cggtgatccc ggaagtgtcg cagctgcggc
     1321 acaccgacaa gcagagcaac ggcggcacga gcaccaacat ccagtcggcg ctcgagctcg
     1381 cctacggact gttcccgccg ggctatctga agcgagagct tttgttgagc gacggcgtcg
     1441 agaccgacgg cgacttgctc tccgaggcaa accgcgcgcg cgtcttcggc gttcaccttt
     1501 cggcgactcc ctttcggcgc ccgcccccca gcgaaatcag cgtcgtacgc ctgactttgc
     1561 cgcaaaaagt cgacatcgga cagaccttcc cggtgatggc ggagatctac gcgagccgcg
     1621 agaacgaggc gcgcctgcgc ttgttccaag gcgaagcgct caacgggctc gacggcgtgc
     1681 gcgacgtgaa gctgcatccc ggtcgcaacg aagtcgaatt caagagcgtc gtgcgcgtcg
     1741 gcggtgacgt cacctacgcg ctcgagctcg agccgcgcgg ccccgacaaa ttcaaagaga
     1801 acaacgcgta ccggacgacg ctcgaggtgc cgagtcgccc gctcgtgctc tacgtcgacg
     1861 gtcaccccga gcgcgcctcg tacctttcga acgcgctgag cgcgcagcag ttcgacgtcg
     1921 acctgcgtgg cccatctgcg tttcccggca gcttgggcga gctcgagcgc ttcgacttcg
     1981 tcgtgctgtc ggatacgccg aaagaggcgt tcggcgcggg ctcggaggag ctcgtggagc
     2041 gctatgtgcg cgatctggga ggcggcttct tgttcgcggg cggacccgct gggtacgggc
     2101 tcggcggttg ggcgcactcg ggcatcgagc gtcttctgcc cgtgcgtatg gacgccgagc
     2161 accgtaaaga gatccccggc gtcgccatgg cgctcgtcat cgatcgctcc gggtcgatga
     2221 cggggctcaa catggagatg gcgaaggcgg cgtgctccgc gaccgtgagt acgttgcaag
     2281 gcgacgactt gatggaggtc atcgccttcg acgccgagcc gcagcgctac gtgaagctcc
     2341 agcccgtgcg gtatcggacg cgcattcaag gtgacatcca gcgtatccag cccggcggcg
     2401 gcaccgagat tttcccggcg ctcgacatgg cctaccaaga cctgtcggtg gtccaagccc
     2461 gtaaaaaaca cgtggttttg ctcaccgacg gtcaggcccc ggtgcaaggc atccgcgagc
     2521 tcgtccaagc gatgatcgcc gaatcgatca cggtgacgac ggttgggctc ggccccgaag
     2581 cgaacgcgga gctcttgcgc tcgatcgcgg atagcggcgg agggcgcttc cacgccgtcc
     2641 cggatccgaa cagcttgccc aagatcttca cgcgcgaaac cgagctcgtc gcgcagcaag
     2701 cggcggtcga ggaatggttt cccgtcgatc aagtcggcag cgccgacttc ttgaagggga
     2761 tctcgatctc cagcgcgccg ttgctccacg ggtatgtctc cacggagctg aagccgccgc
     2821 cggcgcagct catcctgcaa agcgatcgcg gcgagccgat cttggcgcgc tggcgagcgg
     2881 gcctcggctg ggcgctcgcg tggacgtccg acgtgaagaa cggctgggcc gtggagtggc
     2941 tgcgctggcc gaatttcggt cgcttttggg gccaattggt gcgtgagcac atgcgcgtga
     3001 agcggcgccg cgaagtcgac atgaagacgg aggtcgacgg ctcgcacgtc accgcgaccg
     3061 tcgacgcgtt cactcccgac gagcgcttcg acaacgagtg gcagtcgaag ctcatcgtgc
     3121 gacgggtcgg gagcaagggg gagccgcgcg aagtgccgat gcgacaagtc gcgccgggtc
     3181 gctacgcggc ggacttcgac ctcgacgggt ttggctcgtt cttgttgcgc gccgaacacg
     3241 cacggaagga acgcgatggc agcctgcgac cagtcggcgt gagcttcgcg cacgtctcga
     3301 acccataccc tcgcgagtac gcgagcttcg agcccgacat cgagcgactc actcgcgccg
     3361 tgcatgccgc gcggggccaa atcgatccgc cgcccgcgaa gctattcgag ccgcaggggg
     3421 agcaaatcat caagcacgcc gagctctact cgcgcttcgt cttggcttct ctcgtcgcgt
     3481 ttctgctcga ccttctggtg cgccgcgtga ggctgttcga ccgcaaacga ttgaccacgc
     3541 gccgcgcgtg aatccccggc cgctccgcaa acagtgcaga gcgccgaaat tattgcgtgg
     3601 tccgtcgggc gattcgccct tcaaccttcg accgcgaaga gcatactaag gggctgtcgt
     3661 gcgctcgttc acttaccttg cctgcctcgg cacaaaaccc gcagagacgc cggccgtgat
     3721 gcgcccactc ctcggcgaag tgccgg
//
LOCUS       DASTKS010000600         6745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_988935, whole genome shotgun sequence.
ACCESSION   DASTKS010000600 DASTKS010000000
VERSION     DASTKS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988935"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFQ35_12370"
     CDS             complement(<1..584)
                     /locus_tag="VFQ35_12370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931483.1"
                     /translation="MNETHDLFGWVGATLDGKYRIDAVVGEGGFGIVYRAHHLGFDQT
                     VAIKCLRLPKTLTAAERERFRETFLAEGRLLHQLSRANAGIVQALDLGAAVSPNKTWT
                     PYLVLEWLEGTPLDRDFAERRGNPRPVGDAVRLLSSAARALGVAHAQGVAHRDIKPAN
                     LFLANVAGQVVVKVLDFGIAKVMSETESVTKAFE"
     gene            755..1063
                     /locus_tag="VFQ35_12375"
     CDS             755..1063
                     /locus_tag="VFQ35_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9931484.1"
                     /translation="MPSIEFLVNSIGRGKRIDAPEGGELVDICDEYLAPIPFSCRSAS
                     CGTCYVQVLEGGELLEAPGPEEAELVALLGGGADHRLACQARVKPGPGLIRLRAVLGG
                     "
     gene            complement(1084..2070)
                     /locus_tag="VFQ35_12380"
     CDS             complement(1084..2070)
                     /locus_tag="VFQ35_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET9931485.1"
                     /translation="MRVVRVKAPGGPEQLEVVEVADPSPASHEVLIEVHASALNRADV
                     LQRRGLYPPPPGESDVLGLECAGVVRDAGREAGRFPAGSRVMALLGSGGYAECVRVHE
                     NLVMPIPEGMSFEAAAAVPEAFLTAYEALFNAAELAPGERVLIHAGGSGVGIATLQLA
                     RELGAQALCTLRSEPKAERLRALGAARTIVTSTEDFVDVVERETHGRGVDVIVDLVGA
                     AYAANNQRALAAGGRWVAIGLLGGAQATLDFGKLVMKRQTLRGIVMRSRPLPDKTAIV
                     RGFVRDILPWFEAGRLAPVVDSTFPLADVARAHEYMEQNKNFGKIVLTTHAG"
     gene            complement(2073..2960)
                     /locus_tag="VFQ35_12385"
     CDS             complement(2073..2960)
                     /locus_tag="VFQ35_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9931486.1"
                     /translation="MLIGVDFGGTHVKAGVVEGGQVLRSTSAATRDGASPDDVLDLLA
                     GTVLQLTSEPEAVGIAIPGEVDAEGRCWRLTNVPGFAGVHVAAELSRRLGGCPIAVEN
                     DATSAALGERLFGHGREHPSFLMVTLGTGIGAGLVLGHMLYPGANGFAGEFGHLNIDR
                     SPDAPLCACGQRGCVESFSGTKAIVRKYKEYSGIDVDEVRPVAESARRGEKAGLAVFS
                     SMGDALGRGLAAVQNLLDLNAIIFTGGIAAALDILEPHLRTALRSHTFAPPLAEVPLL
                     ASELGERAGIIGAAYLTTL"
     gene            3097..3957
                     /locus_tag="VFQ35_12390"
     CDS             3097..3957
                     /locus_tag="VFQ35_12390"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9931487.1"
                     /translation="MSTLRIATLNIWNKAGPWIERLALIRREVERLQPAVLGLQEVLR
                     FAPNGRERFEPNSATCQASEIGEGFDYHVAYAEASDYGGGLMFGNALLSRFPIVSAQS
                     FILPGLESGESRSLLYTVLETPFGKLPVFVTHLNWKFHQGSVRLKQVRFIAERVAQFV
                     PTRGGFLPPVLMGDFNAEPESDEIRFLRGLAVIEGQSVFFADAWVYGGDGSLGATYDR
                     SNDYARAAREPSRRIDYIFVRGPDSALRGEPMHTELAFATPDVGASGQVWPSDHFGLV
                     SDVYFAPRSP"
     gene            complement(3960..4610)
                     /locus_tag="VFQ35_12395"
     CDS             complement(3960..4610)
                     /locus_tag="VFQ35_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931488.1"
                     /translation="MLVARSAFSGLQHGVDWMVERRSWLALATYGAVLLATGRALAQS
                     QNLAVVQVRGTDPGTTFEIARPGLSSTRARCANPCQITAPQGKYELHVYRGDRELGTA
                     GLKLTGPASFEVSPPNLERQKMGLTLGITGSAFFLAGLVAVAYGTSERHRCGLDAACR
                     DRYESTLWYGAGAAVLGGILSPIGWVLYGTSRSPKVRVLPASQGSRDFSLRAAVTF"
     gene            4742..5050
                     /locus_tag="VFQ35_12400"
     CDS             4742..5050
                     /locus_tag="VFQ35_12400"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HET9931489.1"
                     /translation="MKHVVNHDLGQARAKEVAQSALSSYQEKFAKYSPEVRWQTDNKA
                     NIAFKIKGMTLSGSLEVLEKTIEMDLDVPFMLRPFKNQALGVIEGEIQDWIKKAKTGP
                     "
     gene            complement(5051..6292)
                     /locus_tag="VFQ35_12405"
     CDS             complement(5051..6292)
                     /locus_tag="VFQ35_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9931490.1"
                     /translation="MQAILLLLSLLVALATVGLGLLNLKQLEKPLPALPAALAEVVED
                     ETLLRANAYSRERARLGLATFVASRGLTLVFFFGGAGALYDRLVRAHVDSFVGRGVVF
                     VLGIALFSFLFSLPISLYSNFVLEARYGFNRMTLGLWAGDRVKGALLSGALLSGCAAL
                     ALYLVQAFPTYYWLLAWALLAVFSVLMSMAWPYLIEPLFFKVRPFQDPELEPRIRDLA
                     ERARVHVSRVFQVDASRRSSHSNAYFSGLGPEKRVVLFDTLLARMTPREILAVLAHEL
                     GHYRLHHVLQRFVVGQLLSLAAFAAGAFALAELDPASWVGAHEGSFAFRALIVGFAFS
                     RVEFVATPLFSYWSRHHERQADTFAGKLTRDPAALASGLAKLSRDNLSNLNPHPFYAA
                     FYASHPPAIERISALSRPEPA"
     gene            6502..>6745
                     /locus_tag="VFQ35_12410"
     CDS             6502..>6745
                     /locus_tag="VFQ35_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931491.1"
                     /translation="MSAVKNTTPVEELALDPAALRDSFLRHLQYTQGKHPVAATRLDQ
                     FMAVARSARDRMYDRWTRTWHRRERQQPKVVYYLSLE"
BASE COUNT         1243 a         2181 c         2207 g         1114 t
ORIGIN      
        1 tcctcgaacg ccttcgtcac gctctccgtc tcggacatca ctttggcgat gccgaaatcc
       61 aggactttga cgacgacttg tccggctacg ttcgccaaaa agagattcgc aggcttgatg
      121 tcgcgatgcg cgaccccttg cgcatgtgcg acgccgagcg cccgcgccgc gctggagagc
      181 aagcgcactg cgtcacctac gggccgcggg ttaccccgcc gctcggcgaa gtcgcgatcg
      241 agcggcgtgc cttcgagcca ctcgagcacc agataggggg tccacgtttt gttcggcgat
      301 actgctgccc cgaggtcgag cgcttggacg atgccggcgt tcgctcgcga cagctggtgc
      361 aggaggcgcc cttcggcgag aaaggtttcg cggaagcgct cgcgttccgc cgcggtgagc
      421 gtcttcggca aacgcagaca cttgatggcc acggtctggt cgaagccgag gtggtgcgcg
      481 cgatagacga tgccgaagcc gccctcgccg acgaccgcgt cgatgcggta cttgccgtcg
      541 agcgtcgccc cgacccagcc gaacagatcg tgcgtttcgt tcatcgcgcg gccgagggga
      601 gcgtagcaaa acgcgcgccg cgctccccgg gtttttaccc tcgaggtttg aagggtcgga
      661 gcgggtgggc aaaagcccac aactcccggc catggagccg cggggaagga ctcgattgca
      721 cggtccgagg gcgagcgcta tttcgacgaa aatcgtgccg tcgattgaat tcttggtcaa
      781 ctccatcggg cgtggaaaac gtatcgatgc gccagaagga ggcgagctcg tcgacatctg
      841 cgacgaatac ctggcgccca ttccgttctc gtgtcgctcg gcgagttgcg gcacgtgtta
      901 cgtacaggtc ctcgagggtg gcgagctgct cgaagctccc ggtcccgagg aagcggagct
      961 cgtagcgctg ctcggtggcg gggccgacca tcgtctggca tgtcaggcga gagtgaagcc
     1021 cgggcccggc ctcatccggc tccgcgccgt gctcggcggc tgaccgcgcc gaaactcgac
     1081 gcttcagcct gcgtgcgtcg taagcacgat ttttccgaaa ttcttgtttt gctccatgta
     1141 ttcgtgggct cgcgcgacgt ccgcgagcgg aaacgtcgag tccaccaccg gagcgagtcg
     1201 ccccgcctcg aaccacggca agatatcgcg cacgaagccg cgcacgatcg ccgtcttgtc
     1261 gggcaggggg cgcgagcgca tgacgatgcc gcgcagagtc tggcgcttca tgacgagctt
     1321 gccgaaatcg agcgtcgcct gtgcgccccc gaggagaccg atcgcgaccc agcgtccgcc
     1381 cgccgcgagc gctcgttgat tgtttgcggc gtaggctgcc ccgacgagat cgacgatcac
     1441 gtcgacgccg cgcccatggg tctcgcgctc gacgacatcg acgaagtcct cggtgctcgt
     1501 cacgatggtc ctcgccgcgc cgagggcgcg caggcgctct gctttgggct cgctacgaag
     1561 cgtacagagc gcttgcgcac cgagctctcg cgcgagctgg agcgtggcga tgcccacgcc
     1621 gctgccgccg gcgtggatga gtacccgttc accgggcgcg agctccgcgg cattgaagag
     1681 cgcttcatag gcagtgagaa acgcctccgg taccgcggct gccgcttcga agctcatgcc
     1741 ctccgggatc ggcatcacga ggttctcgtg cacgcgtacg cactccgcgt agccgccact
     1801 gccgagcagc gccatgacgc gcgagccggc agggaaccga ccagcttcgc gtcccgcgtc
     1861 gcgcaccacg ccggcgcact cgagcccgag aacatcgctc tctccgggcg gcggcggata
     1921 gaggccgcgc cgctgcagca cgtcggcgcg gttcagcgcg ctcgcgtgca cctcgatcag
     1981 tacttcgtgt gacgccgggc tcggatctgc gacctcgacg acctcgagtt gctccggccc
     2041 gccgggagcc tttacgcgga cgacgcgcat gctcagagtg tagtcaaata cgcggcgccg
     2101 atgatacccg ctcgctcgcc gagctcactc gcgagcagcg gaacctccgc gagcggcggg
     2161 gcgaaagtgt gactgcggag cgcggtccgc aaatgtggct ccaggatgtc gagcgcggcg
     2221 gcgatcccgc ccgtgaaaat gatggcgttg aggtcgagca ggttctgcac ggccgcgagc
     2281 ccccgcccga gcgcgtcgcc catgctgctg aacacggcga gccccgcttt ttcgccccga
     2341 cgggcgctct cggcgaccgg acgaacttcg tcgacgtcga tgccgctgta ttccttgtac
     2401 ttgcggacga tcgccttcgt tccggaaaag gattcgacgc agccacgctg accgcacgcg
     2461 cagagcggcg cgtcgggact gcggtcgatg ttgaggtggc cgaactcgcc tgcgaagccg
     2521 ttggcgcccg gatagagcat gtgcccgagc accagccccg ctccgatgcc ggtgccgagc
     2581 gtgaccatca gaaagctcgg gtgctcgcgg ccgtggccga acaagcgctc gccgagcgcg
     2641 gcgctggtcg cgtcgttctc tacggcgatg gggcaacctc caaggcgtcg cgagagctcg
     2701 gcagctacgt gcacgccggc gaaaccgggc acgttggtga gtcgccaaca acgcccttct
     2761 gcgtcgactt cgccggggat ggcaatgccg accgcttcgg gctcactcgt gagctgcagc
     2821 accgtgcctg caagcaaatc gagcacgtca tccgggctcg ctccgtcacg cgtcgcagcc
     2881 gaggtcgaac gcagcacctg gccaccttcg acaacgcccg ccttcacgtg cgtccctccg
     2941 aaatcgactc cgatcaacat ggagttcgga acaaatagat gacaccgaaa ctcatcgcaa
     3001 tggcgcccaa attcacgggg gggagtgccc ccgtgagatc caaggtaagg tcgtgtaagc
     3061 gcgcgagcat cgcaaactcc gctactgtag ttgcgcatgt cgacgcttcg gatcgcgacg
     3121 ctcaatattt ggaacaaggc aggcccctgg atcgaacgac tcgcgctgat tcgacgcgaa
     3181 gtcgaacgac tccagccggc cgttctgggg cttcaagaag tgctccgttt cgcgccgaat
     3241 ggccgcgagc gattcgagcc caattcggcg acctgccaag cgtccgagat tggggagggc
     3301 ttcgactacc acgtcgctta cgctgaagct tcggactacg gcggcggact gatgttcggc
     3361 aatgcgctgt tgagccgctt cccgatcgtc agcgcgcaaa gcttcatcct ccccggtctc
     3421 gaaagcggcg aatcgcgctc gctgctctat acggtgctcg agacgccatt cgggaagctc
     3481 cccgtcttcg ttacgcactt gaactggaag ttccatcagg gctcggtgcg gctcaagcaa
     3541 gttcgtttca tcgcggagcg cgttgcgcag ttcgtaccga cgcgcggtgg cttcttgccg
     3601 ccggtgctca tgggagactt caacgcggag ccagagtccg acgagatcag gttcttgcgc
     3661 gggctcgccg tgatcgaggg gcagagcgtg ttcttcgcgg atgcgtgggt ttacgggggc
     3721 gacggcagcc tcggtgcgac ttacgatcgc tcgaacgact acgcgcgcgc cgctcgcgag
     3781 ccgtcgcgac gcatcgacta cattttcgtt cgcggccccg acagcgcgct gcgcggtgaa
     3841 cccatgcaca ccgagctcgc cttcgcgacg ccggacgtcg gcgcgagcgg gcaggtatgg
     3901 ccgtcggatc acttcggcct cgtcagcgat gtgtatttcg cgccgcgttc gccgtgagct
     3961 caaaacgtta cggctgcgcg cagcgaaaag tctcgagagc cctgcgacgc ggggagaact
     4021 ctaactttcg gacttcggct cgttccgtag agcacccagc cgatgggcga caagatcccg
     4081 cccagcacgg cggcccccgc gccgtaccag agcgtacttt cgtaacggtc gcggcacgcg
     4141 gcgtccaatc cgcagcggtg ccgctccgag gtgccatacg cgaccgcgac caagcccgcg
     4201 agaaagaacg ccgaaccggt gatcccgaga gtgaggccca tcttctggcg ttcgaggttc
     4261 ggtggactca cttcgaacga cgccgggccg gtcagtttga ggcccgcggt gccgagctct
     4321 cgatcgcctc gatagacgtg gagctcgtac ttgccttgcg gcgcggtgat ctgacagggg
     4381 ttggcgcagc gcgctcgcgt cgaggacagg cccggacgcg cgatctcgaa cgtcgtgccg
     4441 gggtcggtgc cgcgcacctg aacgaccgcg agattctgcg attgggccaa ggcgcggccc
     4501 gttgcgagca aaacggcccc gtacgtcgca agcgccagcc aggatctccg ttccaccatc
     4561 caatccaccc cgtgttgcag ccccgagaag gccgatcgag caacgagcat gccacaaacg
     4621 gcgcatccgg cgacatcgct ccggggtgct cctcggggtg ccccccgaat gcggcgtttt
     4681 ggcgaaaata ttcgcccttt gcgaaacgcc gccgaatttg ccaagcttcg ccccgccaaa
     4741 gatgaagcac gtcgtcaacc acgatctggg tcaagctcga gccaaagagg tcgcgcagtc
     4801 cgccttgagc tcgtaccaag aaaagttcgc caagtactcg ccggaggtgc gctggcaaac
     4861 ggacaacaag gcaaacatcg ccttcaagat caaaggcatg acgctcagcg gttcgctcga
     4921 ggtgctcgag aagaccatcg agatggacct cgacgtgccg ttcatgctgc gccccttcaa
     4981 gaatcaagct ctcggcgtga tcgagggaga gattcaggac tggatcaaga aggcgaagac
     5041 cggcccctga tcatgcgggt tcggggcggg agagcgcgct gatgcgctcg atcgcagggg
     5101 ggtgcgaggc atagaacgcc gcgtagaagg ggtgagggtt caggttcgac aggttgtcgc
     5161 gcgacaattt cgcgaggcca ctcgcgagcg cggcgggatc gcgagtgagc ttgcccgcga
     5221 acgtgtcggc ttggcgctcg tggtgccgcg accagtagct gaagagaggc gtcgccacga
     5281 actcgactcg cgagaaggcg aagccgacta tcagtgctcg aaacgcgaac gagccctcgt
     5341 gggcgccgac ccagctcgcg ggatcgagct cggcgagcgc aaacgcacct gcggcgaagg
     5401 ccgcgaggga gagcagctgc cctacgacga agcgttgcaa gacgtggtgc agacgatagt
     5461 ggccgagctc gtgcgcgagc accgccaaga tctctcgcgg ggtcatgcgg gcgagcagcg
     5521 tgtcgaacag cacgacgcgc ttttccgggc cgaggccgct gaagtaagcg ttcgaatggc
     5581 tgctacgacg tgaagcgtct acctgaaaga ctcggctcac gtggactcgg gcgcgctcgg
     5641 cgaggtctcg aatgcgcggc tcgagctcgg gatcttggaa gggcctcact ttgaagaaca
     5701 gcggctcgat gaggtacggc cacgccatcg acatgagcac cgagaagacg gcgagcagcg
     5761 cccaggcgag aagccagtag taggtcggaa acgcctggac caagtacagg gcgagcgcag
     5821 cgcagccgga gagcagcgcg ccggagagca gcgcgccttt gactcgatcc ccagcccaga
     5881 gccccagcgt catgcggttg aagccgtaac gcgcttcgag gacgaaattg gagtacaggc
     5941 tgatcggcaa cgaaaagagg aacgagaaca gcgcgattcc gagcacgaag accacgcctc
     6001 gtcccacgaa cgaatccacg tgagctcgga ccaaacgatc gtacagagcc cccgcaccgc
     6061 cgaagaaaaa cacgagcgtg agacctctgc tggccacgaa tgtcgcgagc ccgagcctcg
     6121 cccgctcccg cgaataagcg ttcgctcgca gcagcgtctc gtcttcgacc acctcggcga
     6181 gcgcggcggg taacgccggg agcggctttt cgagctgttt caggttcagg agcccgagcc
     6241 cgaccgtggc gagcgcgacc agcagagaga gaaggagcaa aatcgcctgc actcagcgag
     6301 gctagcattc ggggcagagg acgtgctttc agccggaccg gacgggccgg ctcgctgaat
     6361 cgcaagcgag gcggttctgc ttcagggcgg aattcgtgcc gaaaccggcg attagcctgc
     6421 ccgaaagcgg gtgctgtttc ctagtggcgg tgttcgcgcg ccctttcaaa agtgaggcgg
     6481 gattggtatt cagcaaacat cgtgagcgcg gtcaaaaaca ccacccccgt cgaagagttg
     6541 gctctggatc ctgcggcctt gcgcgacagc ttcctgcgcc acctccaata cacccagggc
     6601 aagcaccccg tcgctgctac ccgtctcgac caattcatgg ccgtggctcg ctctgctcgc
     6661 gaccggatgt acgatcgctg gactcgtact tggcaccgac gcgagcgtca gcagccgaag
     6721 gtcgtttact atctctcgct cgagt
//
LOCUS       DASTKS010000601         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_765563, whole genome shotgun sequence.
ACCESSION   DASTKS010000601 DASTKS010000000
VERSION     DASTKS010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765563"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..906
                     /locus_tag="VFQ35_12415"
     CDS             439..906
                     /locus_tag="VFQ35_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931492.1"
                     /translation="MKKSPVSAVKEQFQSKEKLVEAVQKLATSDLWLDRVNSTKGLAR
                     VSNQKLLRLQAQLTDAKSRFGSRAKLIDAILELEKRVKDAGYKTRLEGFPLPRLLDRH
                     GALSKKQTAATKKPAPKKAAKAAAPAKPAAKAPAKKAPAAAAKKAPAKKAAKN"
     gene            complement(987..1499)
                     /locus_tag="VFQ35_12420"
     CDS             complement(987..1499)
                     /locus_tag="VFQ35_12420"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HET9931493.1"
                     /translation="MPEFRVEHTFNCSEETFWSKIFFDAEYNRRLFLERLKFAGWKET
                     KHEERDGKVHRVVEAIPPIGDLPSALKTVVGEGAGYEEKGVLDHATRRYTADVTPNKL
                     ADKISVRVELSTVADGPNRCKRIAKGSVNVKIFGVGGLLEKKMIGDLEKSYSKSAEFT
                     NEFVKEKSLT"
     gene            1578..>2161
                     /locus_tag="VFQ35_12425"
     CDS             1578..>2161
                     /locus_tag="VFQ35_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9931494.1"
                     /translation="MSSDARAVPLREVSRSTPSWFQQALSVLRKDVTIELSTGEVVVT
                     SGFFGLLIVVIASLAFYGGPTSQRLVGAGVIWVSLSFSAVLALGKTWQRERDESALEG
                     LLLLPLARSAIFAGKALGVLAFLIAISALVIPISALLFSLDLTEVGLGLVCIALAAVP
                     GLAASGTLFGAMTVRTQARDLLLAVVLFPLLTPTL"
BASE COUNT          349 a          698 c          705 g          409 t
ORIGIN      
        1 gcgggcccaa agccagttga agagtgccgt gcgggcgcct ccgatgtgca ggtagccgct
       61 cggcgacggc gcaaagcgca ggcggggctt cacgcggcct ccgtggcgta cgaggcagct
      121 agacgggcga aaaccaaggc cttcatgggc ccccgcgcgt accacgcgcc gcagatagag
      181 tcatcccgcc cctctcggaa ttcggcaggt ttctgcgctc cttcccgaca acggcgccgc
      241 ccgccgcgtc gacgctctcg tggatcgccg agaccgagcg ctccggagag ccgcgccgcc
      301 gggaggaacc gccacgttcc gcagccacta aggagggcct gccaggaatc tccgccattc
      361 cagttgcgac gtcgcgcgca cgttgctaga aggccggcct tctagaggcc cccttcggga
      421 gcggcccgga gtcgaacgat gaaaaagtct cccgtcagcg ccgtcaaaga gcagttccag
      481 tccaaggaga agctcgtaga ggcggtccag aagctcgcca cctccgacct gtggctcgat
      541 cgcgtgaatt cgacgaaggg tctcgcccgc gtctcgaacc agaagctcct gcgcttgcag
      601 gctcagctga cggacgcgaa gagccgcttc ggcagccgcg ccaagctgat cgacgccatc
      661 ctggagctcg agaagcgcgt taaggacgcc ggttacaaga cgcgcctcga aggcttcccg
      721 ttgcctcgcc tgctcgaccg gcacggcgcg ctcagcaaga agcaaacggc cgcgacgaag
      781 aagcccgccc cgaagaaggc ggcgaaggcc gctgctccgg cgaagcccgc cgcgaaggca
      841 cccgccaaga aggcgccggc cgccgccgca aagaaagctc cggccaagaa ggctgccaag
      901 aactgagcgc gctccgggag aggggttcgc cgctctcccg ctcgcagcgg tcagaacacg
      961 agggcgccgt gagcgccctc gttcgtttac gtgaggctct tctctttcac gaactcgttc
     1021 gtgaactcgg cgctcttcga gtagctcttc tcgaggtcgc cgatcatttt cttctcgagc
     1081 agcccgccaa cgccgaaaat cttcacgttg acgctgcctt tcgcgatgcg cttgcagcgg
     1141 ttcgggccgt cggcaacggt cgagagctcc acgcgaacgc tgattttgtc agccagtttg
     1201 ttcggggtta cgtcggcggt gtagcggcgg gtggcgtggt cgagcacgcc tttctcctcg
     1261 tagccggcac cttcaccgac gaccgtcttg agggcgctcg gcaaatcccc gattggcgga
     1321 atcgcctcga ctacgcgatg gacttttccg tcgcgctctt cgtgcttggt ctctttccag
     1381 ccagcgaact tgagtcgctc gagaaagagt cggcggttgt attcggcatc gaagaagatc
     1441 ttcgaccaga aggtttcttc gctgcagttg aaggtgtgtt cgacgcgaaa ttcaggcatg
     1501 ggctccgttc tcgagggtca tcattacaag gtcgtgcgcg gcgcgcaaag ggcggtcgtc
     1561 tggtaagctg acccgagatg tcgtccgacg ctcgagccgt tccgctacgt gaggtgtcgc
     1621 gctcgacgcc gagctggttc cagcaagcgc tgtcggtgct gcgtaaggac gtcacgatcg
     1681 agctctccac cggcgaagtc gtggtcacgt ccgggttttt cgggctgctc atcgtggtga
     1741 tcgcgtcgct cgctttctat ggaggcccga caagccagcg gctggtcggc gccggcgtga
     1801 tttgggtgag cttgagtttc tcggccgtgc tcgcgctcgg caagacgtgg cagcgcgaac
     1861 gggacgagag cgcgctcgag gggctcttgc tccttccgct ggcgcgcagt gcgattttcg
     1921 ccggaaaggc gctcggcgtg ctcgcctttt tgatcgcgat ttcggcgttg gtgatcccga
     1981 tctcggcgct gctgttctcg ctcgacctca ccgaggtggg cttgggtctc gtttgcatcg
     2041 ccctagcggc ggttccgggg ctggccgcgt cggggacgtt gttcggcgcg atgaccgtgc
     2101 gcacccaggc tcgcgacttg ctcctcgcgg tcgtgttgtt tcccttgctg acgcccacgc
     2161 t
//
LOCUS       DASTKS010000602         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_97822,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000602 DASTKS010000000
VERSION     DASTKS010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97822"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFQ35_12430"
     CDS             complement(<1..568)
                     /locus_tag="VFQ35_12430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931495.1"
                     /translation="MTPLLEVGTTLAGKYRIERLLAEGGMGIVYEGYHVELEQRVAVK
                     VVRAEYAHHQEAVARFLNEARAIARLRGHHIAKVLDTGRAETGAPYMVLEYLEGRDLR
                     TVLDNDGPLPVTQAVHYLLQTCEAVAEAHAVGLIHRDLKPDNLFISRGSDGQDLLKVI
                     DFGISKRIDGNGRCLTKQGQSLGSPHYMA"
     gene            918..1451
                     /locus_tag="VFQ35_12435"
     CDS             918..1451
                     /locus_tag="VFQ35_12435"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET9931496.1"
                     /translation="MSQRPFAAFLRGISPMNAKMSELRAAFEAAGFSDVKSVLGSGNL
                     VFRTSSKSETVVERKAEAAMREMLGQSFLTIVRRIESLADLIEGDPFAAFRLRAGSKR
                     IVSFLRSAPIETPRLPIEKDGARIVSLVGSDVFSAYVPSPRGPVFMSLIEKTFGTEIT
                     TRTWETVKKVVAASQRG"
     gene            1490..>2914
                     /locus_tag="VFQ35_12440"
     CDS             1490..>2914
                     /locus_tag="VFQ35_12440"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET9931497.1"
                     /translation="MKDENMRGARKLLFWSSLVLAGCGSDEKPRERPAAAIAALVFTK
                     AAGAQHDSSADAREFFATASNSDIGFVVSDDAGMFNDEQLGVFDAVIFANTSGDVLNS
                     SQQSALERFIASGHGFVGVHSAADTEHDWPFYGRLVGAVATNESAPATVSLRVEDPSH
                     PTAAELPSAFDYEDAWYDFEQTPRRTASVVLSEGSDTSDRPFAWYQYVGGGRSFYTSL
                     GHSSATWLDSRFRNHLLAAVRWAARGPGFGRSILTRQLRSPIALATRPDGDVYVIERT
                     GEVRILRKASGHLDTALALAVDTAQENGLLGLALDPNFAQNDFVYLYYSAPLTGDAVE
                     SGPPGRNVLARYTARADGTLEEGSRQVLLEVPSERRCCHEGGSITFASDGTLWLSVGD
                     NTNPFDSEGTAPIDRRPSRETFNAERTAANPFDLRGKLLRLNSDGSIPAGNLFPATGE
                     LGRPEVYALGVRNPYRLAADPASAR"
BASE COUNT          502 a          930 c          942 g          540 t
ORIGIN      
        1 gcgccatgta gtgaggagag cccaggcttt gaccttgctt cgtcaagcac cgcccgttgc
       61 cgtcgatgcg cttggaaatg ccgaagtcga tcaccttgag aaggtcttgc ccgtccgagc
      121 cgcggctgat gaagaggttg tccggcttca aatcccggtg aatgagccct accgcgtggg
      181 cttccgcgac ggcttcacac gtctgcagca gatagtgaac cgcttgcgtg accggcagcg
      241 ggccgtcgtt gtcgagcacg gttcgaaggt cgcgcccttc gagatactcg agcaccatgt
      301 agggtgcacc ggtctcggcc cgaccggtgt ccaacacctt cgcaatgtgg tgcccgcgga
      361 gccgcgcaat ggcacgcgcc tcgttcaaga atcgagcgac tgcctcttga tggtgggcgt
      421 actccgcccg aacgaccttg accgcgactc gctgctcgag ctctacgtgg tagccctcgt
      481 agacaatccc catcccaccc tcggcgagga ggcgctcgat gcggtacttt cccgcgagcg
      541 tggtccccac ctcgagcaag ggggtcatca gaacggggca accatcgggc attgcgctga
      601 cccgttgcaa ccgccgcacc aactccgagg ctcgtcggtc gatctccgcg tacggcaaac
      661 gtgtctcgcg cgtcgctgaa attcgcgttg ggcgcgctgc attttgcccg tggcgacgtg
      721 gtggtgcggg gcggccgtgc gcctgaacgc tcgagacgac gggccaatgg ggcggttgga
      781 ttcgcctcgc tgaggcgagc cgactcgctc ctgaccgggc gcgtggtcgg tcgcagctcg
      841 gcccgtgtgc cgtgaaatcc gcggccgagg ggccaagctt tacacgcccg aaagatccga
      901 cgaatgatgt cgcccccatg agccagcgac ccttcgccgc atttttgcgg gggatctcgc
      961 cgatgaacgc caaaatgtcc gagcttcgcg ccgctttcga agctgctggc ttcagcgacg
     1021 tgaaaagtgt gctcggtagc ggcaacctcg tgtttcgtac gagctctaag tcggagacag
     1081 tcgtcgagcg gaaggccgaa gccgcgatgc gcgaaatgct cggtcagagc tttctcacga
     1141 tcgtacgacg catcgaatcg ctcgccgacc tcatcgaggg agatcccttc gcggcgttcc
     1201 ggctgcgagc tggttcgaag cgaatcgtga gcttcttgcg cagtgctccg atcgagacgc
     1261 cgcgtctgcc gatcgagaag gacggcgcgc gcatcgtctc gctggtggga agcgacgtct
     1321 tcagcgctta cgtcccgagc ccgcgtggcc cggtgttcat gtctctcatc gaaaagacct
     1381 tcggcacgga gatcacgacg cgcacgtggg agacggtcaa gaaggtcgtc gcggcgtccc
     1441 agcgagggtg agcgaatggg cgaaagcttt ggagccgaga gtcgtcgtca tgaaggatga
     1501 gaatatgcga ggtgcccgca aactattgtt ctggagttcc cttgttctgg ctggctgtgg
     1561 ctccgacgaa aagccgcgcg agcgaccagc ggcggcgatc gcggcgctcg tctttaccaa
     1621 ggccgccggc gcgcaacacg attccagcgc ggacgcccgg gagttcttcg caactgcttc
     1681 caatagtgac atcggcttcg tcgtcagcga cgacgcgggc atgttcaatg acgagcagct
     1741 cggcgtgttc gacgcggtga tcttcgcgaa cacgagcggg gacgtgttga actcttccca
     1801 gcagagtgcg ctcgaacgct tcatcgcgag tgggcacggc ttcgttgggg tgcattcggc
     1861 tgccgacacc gagcacgact ggccctttta cggccgcctc gtaggggcgg tggcaacgaa
     1921 cgagtcagcc cccgcgaccg tgtctctgcg tgtcgaagac ccttctcacc ccaccgcggc
     1981 cgagctgccg tcggcattcg actacgaaga cgcttggtac gacttcgaac agacgccccg
     2041 aaggacggcg agcgtcgtgc tctccgaggg ctctgacacg agtgaccgcc cattcgcttg
     2101 gtatcagtat gtcggcggcg ggcgttcgtt ctacacgagc ttgggtcatt cttccgcgac
     2161 ttggctcgat tcgcgtttcc ggaaccacct cttggcagcc gtgcgttggg ctgcgcgcgg
     2221 tccgggcttt gggcgcagca tcttgactcg ccagctgagg agccccattg cgctcgcgac
     2281 gcgtcccgac ggcgacgtct atgtcatcga gcgcaccggg gaggtgagaa tcttgcgcaa
     2341 agcgagcggc cacctcgaca ccgcgctcgc cctcgccgtc gacaccgctc aagagaatgg
     2401 gctcctcggg ctcgcgctgg acccgaattt cgcgcagaac gatttcgtct acctctatta
     2461 ctccgcgccg ttgaccggtg acgcggtcga gagtggtccg ccgggacgca acgtgctggc
     2521 gcgttacacc gcgcgggccg atggcaccct cgaggaggga agtcgacagg tgctgctcga
     2581 ggtcccgagc gaacggcgct gctgccacga aggaggctcg atcacgttcg cgagtgacgg
     2641 caccctgtgg ctctcggtgg gagacaatac gaacccattc gactcggaag gcactgctcc
     2701 gatcgatcgc cggccatcgc gcgaaacctt caacgcagaa cgcactgctg cgaatccgtt
     2761 cgaccttcgc gggaagctgc tgcgcctcaa ctcggacggc agtattccgg ctggaaacct
     2821 attccctgcg actggagagc tcgggcgccc ggaggtgtac gcgctcggcg ttcggaaccc
     2881 gtaccgactc gcggccgacc ccgcgagtgc acgc
//
LOCUS       DASTKS010000603         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1659021, whole genome shotgun sequence.
ACCESSION   DASTKS010000603 DASTKS010000000
VERSION     DASTKS010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659021"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFQ35_12445"
     CDS             complement(<1..733)
                     /locus_tag="VFQ35_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9931498.1"
                     /translation="MPSPFRILRAAFNSFILPSWTVLCVSAGVIAGTVTRDRDLFYRW
                     QRGWGRGLFRLCGIELEVIGEEHMQRGVPYVIVANHASYMDIPSMFAGLPQPPQFIAK
                     QELARIPFVGTALRWGRHVLIQRGNRSSARESLEQAAQHVSAGAAVLVFPEGTRAVTD
                     EVQKFKTGALRLAKEAKVAILPVGISGTRRVLPKHGRLLYPGHVRVRIGPAIPAEEVA
                     ATDVRTLTQKARSLVADLASLPLAGD"
     gene            complement(850..2262)
                     /locus_tag="VFQ35_12450"
     CDS             complement(850..2262)
                     /locus_tag="VFQ35_12450"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9931499.1"
                     /translation="MGSVVYPFQAPGSGPVSSTDPAEDAAQILKSVTAEDVAIKTLGK
                     CLLPSPVAEHLGEGAMHFVGRADKVLLNDCVSQIDPATLASDRVVALELAGPRNKIFF
                     DPRDVRCGIVTCGGLCPGLNNVIRGLVFELSRGYGVRNILGFRYGYEGILARNGHHPV
                     QLTPESVARIHHEGGTILGSSRGAQDPEKVVDNLQALGIGVLFVVGGDGTLRGALEIA
                     REVERRGARIAVVGVPKTIDNDIHFTDRSFGFESAYAAAVEVIRSARVEASCAARGIG
                     LVKVMGRHSGFLACHAALASTDADLVLVPEVPSVLEGPSGVFTHLERVLTRQGHAVVV
                     VAEGAAQEAIEDDPASGGTDKSGNARLKDVGAFLRDRIVRHFKNEGREVTLKYIDPSY
                     AIRSVPASPSDSVYCWNMARNAVHAAMAGNTEMLIGRWHGRFVHVPMQLATRSRKQLD
                     PAGELWMSLIEATGQPRAFS"
     gene            2568..3398
                     /locus_tag="VFQ35_12455"
     CDS             2568..3398
                     /locus_tag="VFQ35_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931500.1"
                     /translation="MSSTLAPDFSLPPLSRAPNGDGPSKASLRAEREELHWLSIDLRS
                     LSDDVRSALRVSADEIAAWSAYAARFGASLVVVTTETSIELYSTEHDRRRAFRVAMES
                     FAARVELRHELGRCRTVERRGSAAARHLFLRAAGVAPGGGRPFLARMHAATTTAAAHA
                     VLGPTLASLFRIAANVGLRVRVEATNGGTGALGELESLVAERIVEEELAAWQSQEAEL
                     CRAEELSDVFRIQGESEVPSDSRFGEEPGSHVRIRASEYLHGLAEVVPFSLRVAGARK
                     "
BASE COUNT          671 a         1348 c         1367 g          721 t
ORIGIN      
        1 catcgccagc gagaggcaag ctcgcgaggt ctgccacgag gcttcgagcc ttttgggtga
       61 gggtgcgaac gtcggtcgcg gcgacctctt cggctgggat cgcgggtccg atgcgtacgc
      121 gaacgtgacc agggtagaga aggcgtccgt gcttgggcag cactcgtcgt gtcccagaga
      181 tgccgacggg caagatggcg actttggctt ctttcgcgag gcgtagcgcg cccgtcttga
      241 acttttgcac ctcgtcggtc acggcgcgcg tgccttcggg gaacaccaac accgccgcgc
      301 ccgcgctcac gtgctgagcg gcttgttcga gcgactcgcg cgcgctcgaa cggttgccgc
      361 gctggatgag cacgtggcga ccccagcgca gcgccgtgcc gacgaagggg atgcgggcga
      421 gctcttgctt ggcgatgaac tgcggcggtt gaggcagccc cgcgaacatc gacgggatgt
      481 ccatgtagct cgcgtggttt gcgacgatga cgtacggaac gcctctctgc atgtgctcct
      541 cgccgatcac ctcgagctcg atgccgcaca ggcggaacag gccgcgcccc cagccgcgct
      601 gccaacgata gaagagatct ctgtctcgcg tgaccgtccc cgcaatcact cccgcgctga
      661 cgcacaagac ggtccagctc ggcaagatga aggagttgaa tgcggcgcgc aggattcgga
      721 acgggctcgg cacgggcgca ccatatagca ctgcacgtgc tcagcctagg cgctgccgtc
      781 cgccgtgacg cagcgcgccc aaacgggtac ggcgcccttc gcgaactccg cgcgccgtgc
      841 tcttttcgct caggaaaacg cccttggctg ccccgtcgcc tcaatcagcg acatccagag
      901 ctcgccggct gggtcgagct gcttccggct gcgcgtcgcg agctgcatcg gaacgtgtac
      961 gaagcgcccg tgccaccgcc caatcagcat ttcggtgtta ccggccatgg cggcgtgtac
     1021 ggcgttgcga gccatgttcc agcagtagac gctatccgag ggcgaagccg gcacactgcg
     1081 gatcgcgtag ctcgggtcga tgtacttcag cgtgacctcc cgcccttcgt ttttgaagtg
     1141 gcgcacgatg cggtcgcgca ggaacgcacc gacgtccttc agccgagcgt tgccgctctt
     1201 gtcggtgcca ccgctcgccg gatcgtcttc gatggcttct tgcgcggcgc cttcggccac
     1261 caccacgacc gcgtggcctt ggcgagtgag cactcgctcg agatgagtga acacgccgct
     1321 cggcccctcc aacaccgagg ggacttccgg gacgagcacc agatccgcgt ccgtcgacgc
     1381 gagtgcggcg tgacacgcaa ggaagcccga gtgtctcccc atcaccttca cgaggccgat
     1441 ccctcgcgcc gcgcacgaag cctcgacgcg agcgctgcga atgacctcga ccgccgcagc
     1501 gtaggcgctc tcgaatccga agctccgatc ggtgaagtga atgtcgttgt cgatcgtctt
     1561 ggggacgccg acgacggcga tccgcgcgcc tcgacgctcg acttcgcgcg ctatttccaa
     1621 ggcgccgcgc agcgtgccgt caccaccgac cacgaagagc acgccgattc ccaatgcctg
     1681 gaggttgtcc accacctttt cgggatcttg ggctccgcgt gacgatccca gaatcgttcc
     1741 gccttcgtgg tgaatacgcg ccaccgactc cggcgtcagt tgaacggggt ggtgcccgtt
     1801 gcgtgccaaa atcccctcgt acccgtagcg gaagccgaga atgttgcgga ctccataccc
     1861 gcgcgagagc tcgaacacca agccgcgaat cacgttgttg agcccggggc agagcccgcc
     1921 gcacgtcacg atgccgcagc gcacgtcgcg cggatcgaag aagatcttgt tgcggggccc
     1981 cgcgagctcg agggcgacga cgcgatcact cgcgagagtt gcgggatcga tctgcgaaac
     2041 gcaatcgttg agcagcacct tgtcggcgcg gccaacgaag tgcatggcac cttccccgag
     2101 atgttcggcg acgggcgacg gtaacaggca tttgccaagg gtcttgatcg cgacgtcttc
     2161 ggccgtgaca gacttcagga tttgggccgc gtcttccgcc ggatccgtcg aggagactgg
     2221 tccgctccct ggcgcctgaa atggatagac gaccgatccc atgatgttgc tcctctcgcg
     2281 gatttttcgg tgaaccgcgt ttcgaagagc tttccgtccg agggccgccg cgtgacctcg
     2341 gcactgagat tcggggtctg tgtcgcccgc gtagagcgac cgcgagccaa cgcccttgag
     2401 gaaaatctcg gtgtcaagtc gttagcacgg gtttcgccag acccgccggg tcgactgaga
     2461 cccgagaacg ggggtgttga cggacccgct gcgatttccg ccaccgcgaa attcgctgga
     2521 tttttacaga tgggtctcct ggcgcgcccc ttgcttaatc agcagccatg tcctcgacct
     2581 tggcacccga tttcagccta ccgccgctca gccgagcgcc caatggggac ggcccgtcca
     2641 aggctagcct gcgcgccgag cgtgaggagc ttcattggtt gagcatcgat ctccgcagct
     2701 tgtcggacga cgtgcgctct gcactgcggg tgagcgcaga cgagatcgcg gcgtggtccg
     2761 cgtacgcggc tcgcttcggt gcctctctcg tggtggttac gacggagacg tcgatcgagc
     2821 tctactcgac cgagcacgat cgccggcgag cgtttcgggt tgcgatggag agcttcgcgg
     2881 ctcgcgtgga gcttcggcac gaactcgggc gctgccgcac ggtcgagcgc cgtgggagcg
     2941 ctgcggctcg gcacttgttc ctgcgcgccg ccggtgtcgc gccgggcggt ggccggccct
     3001 ttttggcgcg aatgcacgcc gcgaccacga ccgcagccgc tcacgccgtg ctcggcccga
     3061 cgctggcttc gctcttccgc atcgcggcga acgttggcct ccgcgttcgg gtcgaagcga
     3121 ccaacggcgg aaccggcgct ctcggtgagc tcgagtcgct cgtcgccgaa cgcatcgtcg
     3181 aagaggagct agcggcctgg cagtcgcaag aagcagagct ttgccgcgcc gaggagctct
     3241 ccgacgtgtt ccggattcaa ggcgagtcgg aggtcccgag cgatagccgc ttcggtgaag
     3301 agcccggcag ccacgtgcgc attcgcgctt cggaatacct tcacggcctc gccgaagtcg
     3361 tgccgttctc gttgcgcgtg gccggcgcgc gcaagtgacc gagtcgagcg ctctcggcgt
     3421 cagctcgacg ccgagtccgt agcgcggcgc ccgcgcgcgc gtcgctcggc acccgccgaa
     3481 ggcgtctcga cgtgacgtcg cgggagaagc gaccgccgcc cgtcattttc ccgaaacaga
     3541 gaccgcagct gtcgaagcgt ccgcacccga agttcccgga cgacgtgcga atgggtgccg
     3601 cacagcaaca cgcgacgcga ctcgaacacg tgctcctcga cacgtgcgcg gctcgcctcg
     3661 aacctctcgg gggacaggaa attcgaacag atttcgcatc gccgctgcat gcgtggagcg
     3721 tcgcgcggcg gcgggcgcgc ggacaacttt ctggcgagcg tcgcagcgag gctgcaatcg
     3781 caaagcgtat cgctgaaatc gtaagaaatt ccgtagattg atatcctatg gagtgccccg
     3841 gggctttctg attacgtggc ttcatggccg cgagtcgagg cgtgtgggtt cgcgcgagcg
     3901 cgctatgcat ggcactggtc gggtgcggca gtggctccgc tttcaaagac ggatcgctcg
     3961 gtgtcgcggc ttcgcccgcg ggaggaaggg cgaacggtca gggcggcgtg agcgccaccg
     4021 gaacgcgtcc tggctcgcgt ggcggctctt ccggggcgag cggcagcggc gcgatggttg
     4081 cagcaggagg tctgcaaggg ctcggcg
//
LOCUS       DASTKS010000604        13415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1659026, whole genome shotgun sequence.
ACCESSION   DASTKS010000604 DASTKS010000000
VERSION     DASTKS010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659026"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFQ35_12460"
     CDS             <1..1011
                     /locus_tag="VFQ35_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931501.1"
                     /translation="WQDLRVGDVIPAVPGSAEGFEVLRVEPPRVLVLGSSLRDAGRAW
                     DSTWAFALEPLDGSRTRLIARARVKATGMNVRLAWIRPVHHVMESAQLRHLAERAEHR
                     LPRDTLRDRIEGCGGAALMVASFLTPFLRSARSHWGLSEAEATQTRPGDELIPEPTWG
                     WTHAVEIDADANDVWPWISQIGADRGGFYSYQFLENVAGCSLKNAEAIHPEWQVREGG
                     KLSLHPKMPPLSVVSVLPGYGFVAHAAPDEAARARGKPWSSVTWAFFVDDLGAGRSRL
                     VSRYRCATSDDLATRLALGPVCVEPIGFAMDRRMLLGVKERAQRTVARKAAQFTRPTM
                     PR"
     gene            complement(981..1685)
                     /locus_tag="VFQ35_12465"
     CDS             complement(981..1685)
                     /locus_tag="VFQ35_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9931502.1"
                     /translation="MLQTNNLPRSVFRLPPPPLWFVTNGDITVGPVLTDLLIRGVEHG
                     RVPEYCHVRAYEGDWRALLGVREIAALHRKVAYGPSSEQLAEWGRNVERVRDEDELCH
                     TVTWLALITTGAESAMFHYRGRYGRTLFTRSVIGPVNNARLGYPLSELDPVLRAARHG
                     IPVQGPPYGPVEDELAKRFATSQGGVGAAAMIPLLVGGQLVAMLELSRPGHAFRRTDL
                     QRAERIVQRGIVGRVN"
     gene            2089..3567
                     /locus_tag="VFQ35_12470"
     CDS             2089..3567
                     /locus_tag="VFQ35_12470"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET9931503.1"
                     /translation="MSTGISGLDDVLGGGLPERRLYLVKGAPGVGKTTLALQFLTAGA
                     RLGESVLYITLSETESEIRQVAESHGWSLEGIRLFELSSADQTLRLFDENTLYAAEDV
                     DLREIVQVLLELVQKLNPKRVVFDSLSEIRLLSQTPVRYRRQLLALKHFFGERDCTTL
                     LLDEAPDDDGSDLQIESLAHGVILLEQLPVQYGADRRRLRIKKLRGTRFRSGFHDFTV
                     ATGGLSVFPRLVAAEHRTDLLAEPISTGIYGLDSVLGGGLDRATCTLILGPAGAGKSA
                     IATQMAVASAQRGEAASVFLFEERIGTWLRRARQLGMGVDEQVAKGKLHVHQVDPAEM
                     APDEFTHLVRSAVERDGCRQVLIDSITGYFTAMPEARFLTLQMHELLSYLSERGVASV
                     ITMAQSGMIGSQMRSPVDVSYLADTVMVLRYFELEGRLRQALSVLKKRSGAHENTIRD
                     VRFDQRGIHVGETLSELRGVLVGLPHLLGSSQEQEVPSRDFR"
     gene            3554..5098
                     /locus_tag="VFQ35_12475"
     CDS             3554..5098
                     /locus_tag="VFQ35_12475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET9931504.1"
                     /translation="MTSAERERRVDASNAVLVLVRGPRDGELTAELLKQARVACHLCV
                     DVAELCSRIEAGEGAAVLVAEEMLTPPALERLSNILSHQPPWSDFPLLVFGASARSQS
                     ERSEGLIGLGNVTFLDRPVRVRSMIASVRAAVRARDRQYEARRAIESRDSFLAMLGHE
                     LRNPLGAIAFALSVLGKKGVVQGLKEHAVIERQSRHLSRLVDDLLDVARVTHGKVDLK
                     LEKLNLVEVARGAFEALEGRAREQHFSYEFVSDTSTIFVDGDRQRLEQVFANLLTNAI
                     KYTRRGGGIKMRLSCEDEGDLATVAVIDNGVGLAPDMRERVFEPFTQVDESLDRAQGG
                     LGLGLALVRSIVQLHGGSVEAESAGLGHGSTFVVRLHRRADDLPSAPRASPDAVAVTP
                     RKVLVVDDNEDIRELFVVLLRQAGHEVAYAEDGPHGFAEVLRFKPEIAFVDLGLPGFD
                     GLELARRVRAAGARVYLIALTGYGQREDKQRTAEAGFDDHLVKPALDADVERAIRRAN
                     ATLSET"
     gene            complement(5135..5764)
                     /locus_tag="VFQ35_12480"
     CDS             complement(5135..5764)
                     /locus_tag="VFQ35_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9931505.1"
                     /translation="MKSFRLALALVLVATACNTNPSKGKPQASVASAEAPVTATPDAG
                     TVFNFSNDGSTLGFTGAKVTAKHEGTFNTFHGTVRVVDNDPTKSSVNAEIDAASISVE
                     PAKLNNHLKSPDLLDVAKFPKATFVSTAIKPGGDNGATHLITGNLTLHGVAKSITFPA
                     KIFIAPDAVDVESEFAINRKDFGIVYPGMPDDLIKDDVLIKLKIHAKKG"
     gene            5950..6810
                     /locus_tag="VFQ35_12485"
     CDS             5950..6810
                     /locus_tag="VFQ35_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931506.1"
                     /translation="MSMKETQSEASPDLDAGWDELESLSPPAPSRRDSDDDLASIFAV
                     DEGWENEPPAAKPAVPAPIRTALKPPSRPDVAREPRSAAAAEARASEARAVTPIAKEP
                     ASAVEVARASQAVASTASAPAASPKEARLTEARVVSAAELRGLSKKQRRELERRGRAH
                     ASRAKSERKERAKSERKARRGAEQQRLAAERAQAEAARRAEAEQRKQAQKAAKPNQPS
                     AKRVEASADSKPRADAAPAVPRAQRKLEQKVEQKKNVREKSRIAADLSPWLFAVAILA
                     LVAVLALLRH"
     gene            complement(6853..8388)
                     /locus_tag="VFQ35_12490"
     CDS             complement(6853..8388)
                     /locus_tag="VFQ35_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9931507.1"
                     /translation="MKVGVMRGLLREFDFGQVLQVAGIGRQYMEIEISDQNAVVGTIV
                     VKSGKVVSAETPNARGRDAFFQLFGRNDGEFEVFRMQTPEVLPEPVGAVSDLLEEAYD
                     YLKQVDPSSPRLSSRPPKGGELPRNLGLAAPPRISSAEPDFSQSPVVAHVEIAPPPAL
                     TQDAPPSIDHDALGAPPVPQDAVALSDAVPPPALDEALAAAKSPRTASSPPTPARRSA
                     PPERRSVRPPIERNAGEGAPSRHIRTLTASKVVAVASPKGGCGKTTIALNLGLSLARQ
                     GRSVILVDADINGDVLSSINARHRAEIGVYDVLLGVASADEALLDTVLPQFKIMPAVG
                     GQLPRLDAISADHSASWRALSLELAKRADIVLVDTPAGMFGATAQILQSSTHVIGVLQ
                     AEAVANRSFSRFVEALRAMPDERRPEVVGIVVNMLQTRVGASLAVFQSAVTDLPGEWL
                     FETTVPRHRAFLDATHAGLPLRHLDEVAPPAVAWLFDNLAGEVAERLRLPSVEKKPQP
                     LLL"
     gene            complement(8385..8960)
                     /locus_tag="VFQ35_12495"
     CDS             complement(8385..8960)
                     /locus_tag="VFQ35_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HET9931508.1"
                     /translation="MFTNDDKKALLGSWRLVVPIADTAADLFYKRLFELRPEYRELFP
                     EDMSGQKRKLLKMLAFIVKSLDWSDEQWKEDVAPNEDLMLVVLAMGRRHTTLYRVPEE
                     SYGVVGEALMWTLDYGLGEAFTPTVRSAWARLYTYVATAMRMGGAAIQPSSDIVSADE
                     AQRHGTFALLSQQAEAGIDEERLGLPREELS"
     gene            9319..11730
                     /locus_tag="VFQ35_12500"
     CDS             9319..11730
                     /locus_tag="VFQ35_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HET9931509.1"
                     /translation="MMSIGQQHPRTGAFSPCNVTEWSELLSDLGAFIDDESATFAVYS
                     RHAEHVLLEIYEQATGASAVYDYWMARGEGDVFRARIHGVPVGTLYGFRAWGQNWPLS
                     PEWRRGNCIYGFIADVDRDGNRFNPNKLLFDPYARELSHDRETREMKQAGHHAQMYGS
                     GGGLYTGFGHESVVRRAFDTGPFAPKSVLVHDTTTVAPKPLTEQKDLMIYEVHPRGFT
                     RHPSSQRLEQILRGIPGFEEVCNVPTQYRGTYRGAAYMAKYLKALGVNAVEFLPVHES
                     SNALCPDDEPEVDRTPTEPPHGTYWGYMTYGYFAPDRRYAWDQSPGGPTREFKQMVSA
                     FHEAGIAVLLDVVFNHSGEGGIWDDDFQSAEILFLRGLDNAEYYALTGEGSRYYWDST
                     GCGNNLNAARPAVAKLVLDSLEYWTRDMGVDGFRFDLATVLGRGSEGFHFHGGAELLA
                     DIESLAEREDFIAIAEAWDTAPDGYRVGEFPHGWAEWNGIYRDVVRRFLRGDGGQLRA
                     FLEVVNGDYRHFADQGGPHKSVNFITAHDGFTLMDLVSYAHKNNLQPWPFGPCDGGND
                     HNDSCDWGHDKALRRQMLRNFLTILTFSRGVPMLRGGDEFAQTINGNNNPYKIDSVAT
                     WHNFDMIASRAPNRVPTGGSGAYHDNYGLDGNARPVNGLFCFTYELLRLRQRHVCLRQ
                     DKFADFELDAGDDVTYLFKREDSLTDLEGWERCISMHVDGSAIGDRDFVLLINMSSEA
                     VEFRLPAAAPAEPWRRLFDTAHWAEPSANWFDPKQSPRFAGGDSYRVHPWSISGFVQA
                     KPNEV"
     gene            11808..12323
                     /locus_tag="VFQ35_12505"
     CDS             11808..12323
                     /locus_tag="VFQ35_12505"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931510.1"
                     /translation="MATVHLLCGKVGSGKTTYARRLESNGAVRFSVDEWMLRLYGPHM
                     PRKEFDARLSLCLDIIMGLSERLAMLGVPAVVDAGFWRREQRDEARARLERAGVSHCL
                     HFFDVPDDELWRRLELRNRERTSGTFEITREMFELFRGWFEPPGPDEVVHQIDFSRSS
                     GSWPGPPTERK"
     gene            12436..12675
                     /gene="rpoZ"
                     /locus_tag="VFQ35_12510"
     CDS             12436..12675
                     /gene="rpoZ"
                     /locus_tag="VFQ35_12510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET9931511.1"
                     /translation="MARVTVEDCLEKEENRFALVVLAATRCRQLMKGVPSFVNTKNKP
                     AVNALREIAAGRVRFHRPSGEVVNEYIEELSRVVY"
     gene            12675..>13415
                     /locus_tag="VFQ35_12515"
     CDS             12675..>13415
                     /locus_tag="VFQ35_12515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9931512.1"
                     /translation="MAGSRRGKGSSRRGASEHRATPKQPDGDVAPKAGGTRDEYGIFD
                     FGYAGDAGAAAHYQDPDYYTATYANRRHDVDYYVQKARLSRGPVLEYGVGNGRVALHV
                     ARAGIELTGVDLSPAMLEDFRARLEFEPKQVRARVTLVEGDMRTTRLEQKFALVIAPF
                     NAMLHLYDRRDMEAFLAGVRHHLRDDGKFMFDFSLPQPADLARDPERRYPAPPLRHPE
                     THEAVLYAERFEYDPLRQLLLVRMEFSPV"
BASE COUNT         2296 a         4320 c         4460 g         2339 t
ORIGIN      
        1 tggcaagacc tccgcgtcgg tgacgtcatt ccggctgtgc cgggcagcgc ggaagggttc
       61 gaggtgttgc gagtcgagcc accgcgggtg ctcgtgctcg gcagttccct cagagacgcg
      121 ggtcgcgctt gggattcgac ctgggcattc gcgctcgagc cgctcgacgg gtcccgaact
      181 cggctcatcg cgagggcgcg cgtgaaggcc accggcatga atgttcggct cgcctggatt
      241 cgtcccgtgc accatgtgat ggagagcgcg cagctcaggc atctcgcgga gcgcgcggaa
      301 caccgattgc cgcgtgatac gctccgcgat cgaatcgagg gttgtggcgg ggcggcgctg
      361 atggtggcga gctttttgac gccgtttctg cgctcggcgc gatcgcactg gggcctttca
      421 gaggcagaag cgacccagac acgcccggga gacgagctca tcccagagcc cacctggggt
      481 tggacccacg cggtcgaaat cgacgcggac gcgaacgacg tgtggccgtg gatctcgcag
      541 attggcgcag atcggggcgg cttttacagc taccagttcc tggagaacgt cgctggttgc
      601 agcctcaaga acgcggaagc cattcacccc gaatggcagg tgcgcgaagg cggaaaactg
      661 agcctgcacc cgaagatgcc gccgctcagc gtcgtctcgg tgcttcctgg ctacggtttc
      721 gttgcccacg cggcgccgga cgaagcggcg cgcgcccgcg gtaagccctg gagctcggtc
      781 acgtgggcct tcttcgtaga cgatctcggg gctgggcgct cgcgcttggt cagccgctat
      841 cgttgcgcca cctcggacga cctcgcgacg cggctcgccc tcggtccagt ctgcgtcgag
      901 ccaatcgggt tcgcgatgga tcggcgcatg ctgctcggcg tgaaggagcg cgcgcagcgt
      961 acggtcgcca ggaaggcggc tcagttcacg cggccgacga tgccgcgctg aacgatgcgt
     1021 tcggcgcgct gcagatccgt gcgccggaag gcgtgaccgg gccgcgaaag ctcgagcatc
     1081 gcaacgagct gtccgcctac gagaagcgga atcatcgccg ccgcgccgac gccgccttgg
     1141 ctcgtagcga agcgttttgc gagctcgtct tcgaccgggc catagggcgg gccctggacg
     1201 gggatcccgt gacgcgcggc gcgcagcacg gggtcgagct cggagagcgg gtatccgagg
     1261 cgcgcgttgt tgacgggtcc gatgaccgag cgcgtgaaca gcgttcgacc gtaacggcct
     1321 cggtagtgaa acatggcgct ctcggcgccg gtcgtgatga gcgcgagcca agtcaccgta
     1381 tgacagagct cgtcttcgtc tcgcacccgc tcgacgttgc gaccccactc ggcgagctgc
     1441 tccgagctcg gcccgtaagc aaccttgcgg tgaagcgctg cgatctcccg aaccccgagc
     1501 agtgcacgcc agtccccttc gtaggcgcgc acgtggcagt actcggggac tcgcccgtgc
     1561 tcgacgcctc gaatcaacaa gtcggtgaga acggggccga ccgtgatgtc gccgttcgtt
     1621 acgaaccaaa gaggaggagg cggtaaccgg aagacgcttc ggggcaggtt attggtttgc
     1681 agcattggac cggggcaccc tttcgaggcg ccccgaagtc tcttcgcgag acgcggttcg
     1741 ggccaacaaa ccggcgttat gcgatgaaag cgcccgttct cccccgagcc cgtccagcgg
     1801 ctgcacaccc cgggactctg ctgcctcgag agctctgcag cgcgcggcgc ggcggacgag
     1861 cacggagcca agcgtccaga acggcggaaa agcctcggat cgtcaaagag caaatctgca
     1921 tgaactcgcg ggtttgccac gaccggggca aatttgttgt cgcgagtatg cctcgaaaat
     1981 atcggagttg tacggactct gcgtcacgcg ggcgtctggc atagctgtcg catcagggtg
     2041 agggcctgaa tggactcgac cactacgagc aatcagtccc tccgacgcat gagcaccggt
     2101 atctcgggtc tcgacgacgt actcggcggg gggctaccgg aacgaaggct gtatttggtg
     2161 aagggcgcac ccggtgtcgg caaaacgacc ctcgcgcttc agttcctcac ggcgggcgcg
     2221 cgcctcgggg agtcggtact ctacatcacc ctctcggaga ccgaatccga gattcggcag
     2281 gtcgccgagt ctcacggttg gtctctagag ggaatccgtc tgttcgagtt gtcgagcgca
     2341 gaccaaacat tgaggctctt cgacgaaaac accctgtacg cggcggagga cgtcgatctg
     2401 cgcgagatcg tgcaagtgct gctcgagctc gtgcaaaagc tgaacccgaa gcgagtggtg
     2461 ttcgattcgc tgtcagagat tcggcttttg tcacagacgc cggtgcgcta ccggaggcag
     2521 ctgctcgcgc tgaagcactt tttcggagag cgcgactgca cgacccttct gctcgacgaa
     2581 gccccggacg acgacggttc ggacttgcag atcgagagcc tcgctcacgg cgtgattctg
     2641 ctcgagcaac ttccggtgca atatggcgct gaccgccgac gccttcgcat caaaaagctt
     2701 cgcggcacgc gctttcggag cgggtttcac gacttcacgg tggccaccgg cgggctctct
     2761 gtttttccgc ggctcgtcgc ggccgagcac cgcacggacc tcttggccga gcccatctcg
     2821 accggaattt acggcctgga ctcggttctg ggcggcggcc tcgatcgcgc gacgtgcacg
     2881 ctcatcctcg gccccgcggg ggcgggaaaa agcgccatcg cgacgcagat ggcagtcgct
     2941 tcggcgcagc gtggcgaagc ggcgagcgtg ttcctgttcg aagagcgaat cggcacttgg
     3001 ttgcgtcggg cgcgacagct cgggatgggg gtcgacgaac aagtcgcgaa aggaaagcta
     3061 cacgtccatc aggtagatcc ggcggagatg gcgcccgacg agttcacgca cctcgtgcgc
     3121 agcgcggtcg aacgcgatgg atgtcgccag gtgctcatcg atagcatcac cggttacttc
     3181 accgcgatgc ccgaggcccg ctttctcacc ctgcagatgc acgagctcct ctcgtacctg
     3241 agcgagcgcg gagtcgcgtc ggtgatcacg atggctcaat ccggcatgat cgggagtcag
     3301 atgcgctcgc ccgtggacgt gagctatctg gccgacacgg tgatggtcct ccgctacttc
     3361 gaactggaag gtcgcctgcg ccaggcgctc tcggttctga aaaagcgttc gggcgcccac
     3421 gagaacacga tccgcgacgt tcgtttcgac cagcgcggga tccacgtcgg tgaaaccctc
     3481 tccgagctgc gcggcgtctt ggtcgggctt ccccatctct tgggctcatc ccaggaacag
     3541 gaggtgccgt cgcgtgactt ccgctgagcg cgagcggcgc gtggacgcga gcaacgcggt
     3601 gctggtgctg gtgcggggcc cgcgtgacgg agagctaacg gcagagcttc tgaagcaggc
     3661 tcgcgtcgcg tgtcacctat gcgtagacgt ggccgagctg tgtagccgca tcgaggccgg
     3721 cgagggcgcg gccgtgctcg tcgcggaaga gatgctgacc ccacccgcgc tcgagcgcct
     3781 gagcaacatt ctgtcgcacc agccaccgtg gtccgacttt ccgttgctgg tcttcggcgc
     3841 gtcggcgcgg agccagtcgg agcggtccga agggctgatc gggctcggta acgtcacgtt
     3901 cctggaccgt cccgtgcgcg tgcgctccat gatcgcctcg gtccgggcag ccgtccgcgc
     3961 ccgcgaccgt cagtacgaag cgcggcgcgc catcgagtcg cgagactcgt tcttggcgat
     4021 gctcggtcac gagcttcgca atccgctcgg cgcgatcgcg ttcgcgctct cggtgctagg
     4081 aaagaagggc gtcgttcagg gcctcaaaga gcacgccgtc atcgagcggc agtcgcgtca
     4141 cttgagtcgc ctggtcgatg accttctcga cgtcgcccgc gtgacccacg gcaaagtcga
     4201 cttgaagctc gagaagctca acctggtcga ggtggcgcgc ggcgccttcg aggcgctcga
     4261 gggtcgcgct agggagcagc acttttctta cgagttcgtg agcgacacga gcaccatttt
     4321 cgtggatggc gatcgtcaac ggctcgaaca agtgttcgcg aacctgctga ccaatgcgat
     4381 caagtacact cgtcgcggcg gtggcatcaa aatgcggctc tcgtgcgagg acgaagggga
     4441 cctcgccacg gtcgccgtga tcgacaacgg cgtgggcctc gcacccgaca tgcgcgaacg
     4501 cgtcttcgaa ccgttcactc aagtcgacga gtcgctcgat cgcgcgcagg gcggtctcgg
     4561 tctcggcttg gcgctcgtgc gcagcatcgt tcagctccac ggtggtagtg tcgaagccga
     4621 gagcgctggc ttgggacatg gcagcacgtt cgtcgtgcgc ctccatcggc gcgccgacga
     4681 cctgccctcg gcgccgcgcg cgagccccga cgcagtcgcg gtcacgccgc gaaaggtgct
     4741 cgtggtcgac gacaacgaag acatccgcga gctcttcgtg gtgctcttgc gtcaagccgg
     4801 ccacgaggtc gcctacgccg aggacggtcc ccacgggttc gccgaagtgc tgcgattcaa
     4861 gccagaaatt gcgttcgtgg acctcggtct gcccggcttc gacggcctcg aactcgcgcg
     4921 gcgagtgcgc gcggcgggag ctcgcgtcta cctcatcgcg ctcactggct atgggcagcg
     4981 cgaagacaag cagcgaacgg cggaggccgg cttcgacgac catctggtga agccggcgct
     5041 cgatgccgac gtggagcgcg cgatccgtcg cgccaacgcg acgctctcgg agacatgatc
     5101 gagagaccga gagcgccggt ggctcctccg cgactcagcc cttcttcgcg tgaatcttca
     5161 gcttgatgag gacgtcgtct ttgatgaggt cgtccggcat cccggggtag acgataccga
     5221 agtcttttcg gttgatcgca aactcggatt cgacgtccac ggcgtcggga gcgatgaaga
     5281 ttttggccgg gaaagtgatc gatttcgcga cgccgtgcag cgtgaggttc cccgtgatga
     5341 ggtgcgtcgc gccgttgtcg ccgccgggtt tgatcgcggt cgagacgaac gtcgctttgg
     5401 ggaacttggc cacgtcgagc aggtccggcg acttcaagtg attattgagc ttggccggtt
     5461 ccacggagat cgaggcggcg tcgatctctg cgttcacgct gctcttcgtg ggatcgttgt
     5521 cgactactcg caccgttccg tggaaggtgt tgaaggtgcc ttcgtgtttg gcggtcacct
     5581 tcgcgccagt gaatcccagc gtcgagccgt cgttgctgaa gttgaagacg gtccccgcgt
     5641 cgggcgtagc ggttacgggc gcctcggcgc tcgcgacgct ggcctggggc ttgcccttgg
     5701 atggattggt gttgcaagca gtggcgacca gcacgagtgc caaagcgaga cgaaaggatt
     5761 tcattgggcc tcctcttcga ggagggtagt catgaaaccc ggccccgccc aagacaatgg
     5821 ttttgcgaga taattcgtga cggagcgtcg ccgctccaag tcgcctcgcc gagctctgcc
     5881 gcgatcgacc gaaccgctta tcgtggcgtg tgcgagtcag ctcgattagg ctccgcctcg
     5941 attcggccga tgagcatgaa ggaaacccag agcgaagctt cgcccgatct cgatgcaggt
     6001 tgggacgagc tcgagtcgtt gagccctccg gctccgtcgc ggcgtgattc ggacgacgac
     6061 ctcgcctcaa tcttcgcggt ggacgagggc tgggagaacg agccgcctgc ggcgaaaccc
     6121 gccgtgcccg cgccgatccg taccgccctc aagcctccgt cgcgaccgga cgtcgcgcgc
     6181 gagccgcgtt cggcggcagc ggctgaagcg agagcttccg aggcgcgagc agtgacgccg
     6241 atcgcgaagg agcctgcgtc ggcggtcgag gtggcgcgcg catcgcaagc cgtcgcgtcg
     6301 accgcgagcg cgccggccgc gtcgcccaag gaagcgcgcc tgaccgaagc gcgagtcgtg
     6361 tcggccgccg agctgcgtgg cctcagtaag aagcagcggc gtgagctcga gcgtcgcgga
     6421 cgagcgcacg cttcgcgcgc gaagtcggag cgtaaggagc gcgcgaagtc ggagcgcaaa
     6481 gcccgccgtg gcgccgagca gcagcggctc gcggccgagc gcgctcaggc ggaagcggcg
     6541 cggcgcgcgg aggcggagca acgaaagcag gcccagaagg cggcgaagcc gaaccagccg
     6601 agcgcgaagc gcgtcgaagc gagcgccgac tcgaagcctc gcgccgacgc ggcgcccgcc
     6661 gtaccgcgtg cgcagcgcaa gctcgagcaa aaagtcgagc agaagaagaa tgtacgcgaa
     6721 aagtcgcgta tcgcggcgga tctctcgccg tggctattcg cggtcgcgat cctcgcgctc
     6781 gtggcggtgc tcgcgttgct acggcactga gcgcaccctc gttcgatggc ccgacgaggg
     6841 cgaagccgcg cgtcagagca agagcggctg cggcttcttc tcgacgctgg gcaatcgcag
     6901 gcgctcggcc acctcaccgg cgaggttgtc gaagagccaa gcgaccgccg gcggcgcgac
     6961 ctcgtcgagg tgacgcagcg gtaggccagc gtgcgtcgcg tcgaggaacg cgcgatggcg
     7021 cggcaccgtc gtctcgaaca gccattctcc cggcagatcc gtcactgcgc tctggaaaac
     7081 ggcgagggac gcgccgacgc gtgtttgcag catgttcacg acgatgccga cgacctcggg
     7141 gcggcgctcg tcgggcatcg cgcgaagcgc ctccacgaag cgcgaaaacg agcgattcgc
     7201 caccgcctcg gcttggagca ctccgatcac gtgcgtcgaa gactgaagga tctgtgccgt
     7261 cgctccgaac attcctgcag gcgtatcgac cagcacgatg tcagcgcgtt tcgcgagctc
     7321 gagtgagagc gctcgccagc tcgccgagtg atccgccgag atcgcgtcga ggcgaggcag
     7381 ctggccgcct accgcaggca tgatcttgaa ctgggggaga acggtgtcga gcagcgcttc
     7441 gtctgcgctc gcaactccga gcagcacgtc gtaaacgcca atttcggcgc ggtggcgcgc
     7501 gttgatcgac gacagcacgt cgccgttgat gtcggcgtcc accaggatca ccgagcgccc
     7561 ctgtcgcgcc aaactgaggc cgaggttcag cgcgatggtc gtcttgccac agccaccctt
     7621 ggggctcgcg accgcgacga ccttcgacgc cgtgagcgtg cgaatgtggc gcgacggcgc
     7681 gccttcgccg gcgttgcgct ctataggagg gcgcacgctg cgccgctctg gcggtgcgct
     7741 gcgtcgcgct ggcgtgggcg ggctcgacgc cgtgcggggg ctcttcgccg ccgccagcgc
     7801 ttcgtcgagc gcgggtggcg gcaccgcgtc gctcaatgca actgcgtcct gtggcaccgg
     7861 cggggcgccc agcgcgtcgt gatcgatgga cggcggcgcg tcttgtgtga gcgctggcgg
     7921 cggcgcgatc tcgacgtgcg caactacggg cgattggctg aaatccggct cggcagagct
     7981 aatccggggc ggcgcagcga gccccaggtt gcgcggcagc tcgccacctt tgggcggacg
     8041 gctcgaaagg cgcggcgagc tcgggtcgac ttgcttcaag tagtcgtagg cctcttccag
     8101 tagatcgctc acggcaccga ccggttcagg cagcacctcg ggtgtctgca tgcggaacac
     8161 ctcgaactcg ccatcgttgc gcccgaagag ctggaagaag gcgtcacgac cgcgcgcgtt
     8221 cggggtctcg gcgctgacga cctttccgct cttcaccacg atcgtgccga ccaccgcgtt
     8281 ctggtcgctg atttcgatct ccatgtactg gcggccgatg cccgcgactt gaagtacctg
     8341 accgaagtcg aactcgcgga gcaacccgcg catcacaccg actttcacga cagctcctct
     8401 cgcgggaggc cgagtcgctc ttcgtcgatg ccggcttcgg cctgctgaga aagcagcgcg
     8461 aacgtgccgt gtcgctgcgc ttcgtctgcg ctcacgatgt cgctcgaggg ctgaatggca
     8521 gcgccgccca tgcgcatggc ggtcgcgacg tacgtgtaca ggcgagccca cgcgctcctg
     8581 acggtgggcg tgaaggcttc cccaagccca tagtcgagcg tccacatcag agcttcgccc
     8641 acgacgccgt aggactcctc cggaacccga tacaaggtcg tgtggcgcct tcccatcgcg
     8701 agcacgacga gcatcagatc ttcgttggga gcgacgtctt ccttccactg ctcgtcggac
     8761 caatcgaggg acttgacgat gaacgccagc atcttcaaaa gcttgcgttt ctgtcccgac
     8821 atgtcttccg gaaacagctc ccggtactcg gggcgaagct cgaacaggcg cttgtaaaag
     8881 aggtccgcgg cggtgtcggc gatcgggacg acgaggcgcc agctgccgag taacgctttc
     8941 ttgtcatcgt tcgtgaacac gagcgctcct gcgaagagcc cgcatgagcc gagcgggcga
     9001 ccaccaggag tagttcacac gcgcgcctac ggcaacggcc agcaaagggc cagaggagtg
     9061 acgaatgtcc cttcgatttc tcgatttacg caattttcgg cgtaaaagcg taggctactt
     9121 gtcaggtgtc ggggccagaa ccagctcctc agagctcgct cgtcaggtag gcttcgtctc
     9181 cttcgctcct cgaaacctcg gcatgaaaga gttcactacc attggggccg tgagcggctg
     9241 tgttcggatg cttgtttggt ttgcctctcg gggtgtccca agttcctgcc cgtcgcgaga
     9301 ggttccgagg tctgacgaat gatgagcata ggacaacagc accctcgtac cggggccttc
     9361 tcgccctgca acgtcactga atggtcggag ctcctctcgg atctcggcgc gttcatcgac
     9421 gacgagagcg cgacgttcgc agtttactca cgccacgccg agcatgtgct gctcgagatt
     9481 tacgagcaag caacgggcgc gagcgcggtt tacgactatt ggatggcgcg cggcgagggc
     9541 gacgtgtttc gcgcgcgaat tcacggcgta cccgtgggaa cgctgtatgg atttcgcgcc
     9601 tgggggcaga actggcccct ctcgccggag tggcggcgcg gcaactgtat ttacggcttc
     9661 atcgccgacg tcgatcgcga cggtaaccgc ttcaacccga acaagctgct gttcgatccc
     9721 tacgcccgcg agctctcgca cgatcgggag acccgggaga tgaagcaagc gggtcaccac
     9781 gcgcagatgt acggctcggg tggcggcctc tacaccggct tcggtcacga aagcgtggtt
     9841 cgtcgagcct tcgacaccgg cccgttcgcg ccgaaatcag tgttggttca cgacacgacg
     9901 accgtcgccc cgaagccgct caccgaacag aaagatctca tgatttacga ggtgcacccg
     9961 cgcggcttca cgcggcaccc ttcttcgcag cgcctcgaac aaatcttgcg tggcatcccc
    10021 ggcttcgagg aggtgtgcaa cgtgccgacg cagtatcgcg ggacgtaccg cggcgccgcg
    10081 tacatggcga agtacctgaa ggcgctcggc gtgaacgcgg tggagtttct ccccgtccac
    10141 gagagctcga acgcgctctg ccccgacgac gaacccgagg tggaccgcac cccgacggag
    10201 ccgccgcacg gcacctattg gggatacatg acgtacgggt atttcgcgcc cgatcgtcgc
    10261 tacgcctggg accagagccc tggcgggccg acgcgcgaat tcaagcaaat ggtcagcgct
    10321 tttcacgagg ccggcatcgc ggtgctgctc gacgtcgtct tcaaccacag cggcgagggc
    10381 ggcatctggg acgacgactt tcaaagcgcc gagatcttgt tcttacgcgg cctcgacaac
    10441 gccgaatact acgcgctcac cggcgagggt tcgcgctact actgggacag caccggctgc
    10501 ggcaacaacc tcaacgcggc gcgaccggcg gttgccaagc tcgtgctcga ctcgctcgaa
    10561 tactggacgc gcgacatggg cgtcgacggc ttccgcttcg atttggcgac ggtgctcggc
    10621 cgcggctcgg aaggcttcca ttttcacgga ggtgccgagc tcctcgccga catcgagagc
    10681 ttggccgagc gggaagactt catcgcgatc gcagaggcgt gggacactgc tcccgacggc
    10741 taccgcgtcg gcgagttccc gcacggctgg gccgagtgga acggtatcta tcgcgacgtc
    10801 gtgcgccgct ttcttcgagg ggacggcggt cagctgcgcg cgttcctcga ggtcgtaaac
    10861 ggagattaca ggcacttcgc ggatcaggga ggcccccaca agtcggtgaa cttcatcacc
    10921 gcccacgacg gcttcacgct gatggatctc gtgagttacg cccacaaaaa caaccttcag
    10981 ccgtggccct ttggtccctg cgacggcggt aacgaccaca acgactcgtg cgactgggga
    11041 cacgacaaag cgttgcgccg gcagatgtta cggaatttcc tcacgatcct cacgttctcg
    11101 cgcggcgtgc cgatgctgcg cggcggcgac gagttcgcgc agacgatcaa cggcaacaac
    11161 aacccctaca aaatcgacag cgtcgccacg tggcacaact tcgacatgat cgcgtcgcgc
    11221 gcccccaacc gggtgccgac gggtggtagc ggcgcgtatc acgacaacta cggcctcgac
    11281 ggcaacgcgc gacccgtgaa cggcttgttc tgcttcactt acgagctcct gcgcttgagg
    11341 cagcgccacg tctgcttgcg ccaggacaag tttgccgact tcgagctcga cgcgggcgac
    11401 gacgtcacgt acctgttcaa gcgcgaagat tcgctgacgg atctcgaggg ttgggagcgc
    11461 tgcatctcga tgcacgtcga cggcagcgcc atcggcgatc gcgatttcgt gctgctcatc
    11521 aacatgagct cggaggcggt cgagtttcgg ttgcccgctg ccgcccccgc cgagccgtgg
    11581 cgacgcttgt tcgacacggc gcactgggcc gagccctcgg cgaattggtt cgatcccaag
    11641 cagtctccgc gctttgccgg tggcgactcg tatcgcgtgc acccgtggtc gatctccggc
    11701 ttcgttcagg ccaaaccgaa cgaggtttga ccggcgatcc gtgagttgga tccgtcacgc
    11761 cgcgtttgac tcgggccgtt ccccttacca gactgcggcc gaacgcgatg gctaccgttc
    11821 acctcttgtg cggcaaggtc ggctcgggaa agacgactta cgcacggcgc ctcgagtcga
    11881 acggtgcggt gcgcttctcg gtcgacgagt ggatgttgcg cctctacggc ccgcacatgc
    11941 cgcgcaaaga gttcgatgcg cggctctcgc tctgcctgga catcatcatg ggcctctccg
    12001 agcgcctcgc gatgctcgga gtgcccgcgg tcgtggacgc tgggttttgg cgccgcgagc
    12061 agcgcgacga ggcgcgcgca cggctcgagc gggcgggcgt gagccactgc cttcacttct
    12121 tcgacgttcc ggacgacgag ctttggcgcc gtctcgagct gcgtaaccgc gaacgtacgt
    12181 cgggcacctt cgaaatcacc cgcgagatgt tcgagctctt ccgggggtgg ttcgaaccgc
    12241 ccggtcccga cgaggtcgtg caccaaatcg attttagccg gagttcaggc tcctggccgg
    12301 ggcctcccac tgaacggaag taggggcatc cattcggtag ctgcggcggt acggtaagta
    12361 ccctcggaac cacgacttgc gaagcgggcc gccgggggct agcctgccgg ccctttcgga
    12421 agaggctcac caccaatggc tcgcgttacc gtcgaagatt gcttggagaa ggaagagaat
    12481 cggttcgcac tcgtcgtgct cgcggccacc cgctgccgcc agctgatgaa gggcgtgccc
    12541 tctttcgtga acacgaagaa caagcccgcc gtgaacgcgc tgcgtgagat cgcggccggt
    12601 cgcgtgcgct tccaccgccc gtcgggcgaa gtcgtgaacg agtacatcga agagctgagc
    12661 cgcgtcgttt actgatggcc ggctcacgtc gcggcaaagg gtcgtcgcga cgaggagcgt
    12721 ccgagcatcg cgcgacgccc aagcagccgg acggggacgt cgcgcccaaa gccggaggca
    12781 cgcgcgacga gtacggcatc ttcgacttcg gttacgccgg agatgccggg gccgcggcgc
    12841 attaccaaga ccctgattac tacaccgcga cgtacgccaa tcgacgccac gacgtcgact
    12901 actacgtcca aaaagcccgg ctttcgcgcg gccccgtgct cgaatacggc gtcgggaacg
    12961 ggcgcgtggc gctgcacgtc gctcgcgccg gcatcgagct caccggggtc gatctctccc
    13021 ccgcgatgct cgaggacttc cgcgcccggc tcgagttcga gccgaaacag gtgcgcgcgc
    13081 gcgtaaccct ggtcgaaggc gacatgcgca ccacgcggct cgagcagaag ttcgcgctgg
    13141 tgatcgcgcc gttcaacgcg atgctgcacc tttacgatcg ccgcgacatg gaggccttct
    13201 tggccggcgt gcggcatcac ctgcgcgacg acgggaagtt catgttcgac ttctcgctcc
    13261 cccagcccgc ggacctcgcg cgcgaccccg agcgtcgcta ccccgcgccc ccgctgcgac
    13321 acccggagac ccacgaggcc gtcctttacg cagagcgctt cgaatacgac ccgctccgcc
    13381 agctcttgct cgtgcgcatg gagttctcgc cggtc
//
LOCUS       DASTKS010000605         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_98100,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000605 DASTKS010000000
VERSION     DASTKS010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98100"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFQ35_12520"
     CDS             complement(<1..708)
                     /locus_tag="VFQ35_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931513.1"
                     /translation="MKPPLYAGANAHPAQATCRLRRRSNQRLRFLSERGHRGATSSNL
                     PVLLGSPCGAALIATIANALARSTQLEQGFAVALQLLMDATGSALAAVDMLRGNAQPP
                     LRVEVSRGENKLPHFQSESQLILAISRIDRASELAPDEVDRVLGVQEREHPRVEDMVI
                     APIGEPLARRGLLWLQRPSESFTSEDWRSFAEGVASQVSQGLERRQTEAELSEHARLA
                     TLFADVSIALASTASLEV"
     gene            complement(897..1106)
                     /locus_tag="VFQ35_12525"
     CDS             complement(897..1106)
                     /locus_tag="VFQ35_12525"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9931514.1"
                     /translation="MKKNLVIFDRLLRMAAGAALLLAPLLDIRVYPVNLLGVVLIATA
                     LVGYCPLYTAMGWVARSFGSNRHAS"
     gene            1552..1962
                     /locus_tag="VFQ35_12530"
     CDS             1552..1962
                     /locus_tag="VFQ35_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9931515.1"
                     /translation="MSKVIPTVQKYMTTSPHTIGEEQPMAVAHRIMRDNRIRHLPVLR
                     DGKVAGMVSDRDLHLIETLKDVDPREVQVSEAMSQDPYVVAPDAPLDEVVRTMAEKKY
                     GSAIVVQSQKVVGIFTTVDVCRAFAELLHTRLAK"
     gene            2133..>2764
                     /locus_tag="VFQ35_12535"
     CDS             2133..>2764
                     /locus_tag="VFQ35_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9931516.1"
                     /translation="MKPAASESVQVLVVGAGPVGLFAAFCAAQRGLQVLVIDHVWRGY
                     ASGHASLVHSGFLDLLEDGPLLQKLRTEGHFLDRVELDIDGAEVAVCELPSPALVIPQ
                     RVLEEGLCAELRKMDVEIRSPHQAATIEQRASHVDVRVLRRELVTSGSPVLHSEWEPV
                     ESLLVRADFVIGADGYDSRVRAALGIDVVDFGGIESFAMFEGVARRPPKAT"
BASE COUNT          513 a          847 c          915 g          489 t
ORIGIN      
        1 aacctcgaga gatgcggtgg acgccagcgc gatgctcacg tcggcgaaca gcgtcgcgag
       61 ccgtgcgtgc tcgctgagct cggcttcggt ttgacggcgt tcgagcccct gagacacctg
      121 actcgcgacg ccctccgcga aagagcgcca gtcttcgctc gtgaaggact ccgacgggcg
      181 ctggagccat agcaggccac gtcgcgcgag gggctctcca atcggcgcga tcaccatgtc
      241 ttccacccgc gggtgctcgc gttcctgcac cccgagtacg cgatccactt cgtcaggagc
      301 tagctcgctg gcgcggtcga tgcgcgaaat ggcgaggatg agctgcgact cgctctgaaa
      361 atgaggcagc ttgttttctc cgcggctgac ctcgacccgg aggggcggct gcgcgttacc
      421 ccgcagcatg tcgacggcgg cgagcgccga cccggtcgcg tccatcaaca actggagagc
      481 gaccgcaaag ccctgctcga gctgcgtgct tcgcgcgagg gcgttcgcaa tggtcgcgat
      541 gagcgcggcg ccgcagggag agccgagcag caccggcaga ttcgagctag tggcgcctcg
      601 gtggccgcgc tccgacagaa agcgaagtcg ctggttcgag cgtcgcctca gccggcacgt
      661 ggcttgcgcg gggtgtgcgt tcgcaccggc gtagagcggc ggtttcattc gagcattcat
      721 tgcgcagcaa ttcgcgaaag gatcgctgcc gacgtgcggg tagaggagtc gccggcgacg
      781 acctgtgacg accaaaactg ggacgggcgc ctcgatggat tcattagatt gcggggctcg
      841 gtcaccccct aaaaaccaaa gagcgatgga ggctacgtct cgaagacgga accctcctag
      901 gaagcgtgac ggttggagcc gaagcttcga gcgacccaac ccatggcggt gtagagcgga
      961 cagtacccca cgagggcagt ggcgatgagc accacgccga gcaggttcac cggatagact
     1021 ctgatatcga gaagaggtgc gagaagtagc gctgcgcccg cggccatgcg cagcagccgg
     1081 tcgaagatga cgaggttctt tttcacgaag gccctctcat gaaccccaca agcagcaagc
     1141 gtgcctctcg gccctcgcgg gaaaatgccg caaggagccg cgcagaaagt gcgtttggag
     1201 tctcgacccg cgcaaattgc gctgcgcgct tcgagcacgc aatcagcgtt caagcgccgt
     1261 tcgcagcttc actctcgggt gtggggtgtt ggaacctcag gatttatcgg atctccgaac
     1321 cacgcatcgg tccgccgttt tcggggtttc tggggccccg aaaaccatac aaaccaaagc
     1381 atcggaacga actctttcga agcacgggtc ggaaccaact ctttcgtgtc gagccctctg
     1441 cgagcgaacg agacgaagac ctacctgctg gcgacatgtg acgaatcctg aggttggaac
     1501 acaccttgca gcgccctgtc gagcgagcca ccaaattcac tggagacttc gatgagcaag
     1561 gtcattccga cggttcaaaa gtacatgacg acttcgccgc acaccatcgg tgaggagcag
     1621 cccatggccg tcgctcaccg catcatgcgc gacaaccgca tccggcactt gcccgtgctg
     1681 cgagacggca aagtcgcggg catggtttcc gatcgtgact tgcacctcat cgagaccctg
     1741 aaggacgtcg atccgcgcga agtgcaagtg agcgaagcca tgtcgcagga tccttacgtg
     1801 gtcgcgccgg acgcgccgtt ggacgaagtc gttcgcacga tggccgaaaa gaagtacggc
     1861 tcggccatcg tcgttcagtc gcagaaggtg gtagggattt tcacgaccgt cgacgtgtgt
     1921 cgagcgttcg cggagctcct gcacacccgg ctcgcgaagt agcgggacct ttcgccgatg
     1981 cacgcagtct tttccggctc tctcgggccg cgcgatacgt ttgcgccgac gcgaagcgat
     2041 tgcgccctgt cgcgtcggaa atctggcagt gacgacccgc atttagcggt ggcgtcgctg
     2101 gcccgatctt tgcgtagcca gcgaggatgc tggtgaagcc tgcggctagc gaaagcgtcc
     2161 aggtgctcgt agtgggcgcg gggccggtgg gtcttttcgc ggcgttttgt gcggcgcagc
     2221 gcggcttgca ggtgctcgtc atcgaccacg tctggcgcgg gtacgcgagc ggccatgcat
     2281 cgttggttca ctcggggttc ctggacctgc tcgaggacgg ccctctgttg caaaaactcc
     2341 gcacggaggg gcacttcctg gatcgagtcg agctcgacat cgacggcgcc gaggtcgcag
     2401 tctgcgagct cccgtctccc gcgctggtga tcccgcagcg cgtcctggaa gaagggttgt
     2461 gtgccgagct gcggaaaatg gacgtcgaga tccgaagtcc acaccaggcg gcaacgatcg
     2521 agcaacgggc atctcacgtc gacgttcgcg tgttgcggcg ggagctcgtc acctcgggct
     2581 cgccggtttt gcacagcgaa tgggagccgg tagagtcgct cctggtgcga gccgacttcg
     2641 tcatcggggc agacggatac gactcgcgcg tgcgcgcggc ccttggcatc gacgtcgtcg
     2701 acttcggcgg catcgagagc ttcgcgatgt tcgaaggggt cgcgcgccgc ccgccgaagg
     2761 cgac
//
LOCUS       DASTKS010000606         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1883796, whole genome shotgun sequence.
ACCESSION   DASTKS010000606 DASTKS010000000
VERSION     DASTKS010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883796"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2347)
                     /locus_tag="VFQ35_12540"
     CDS             complement(<1..2347)
                     /locus_tag="VFQ35_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET9931517.1"
                     /translation="MADHPESTPSRWTRYRPTDLSAMLSCEHLVALERGVHEGALERT
                     FRDDPALELLRLRGLSHERRYLDELAARGRKVVSIAADAEQRDAQFWARGIFQTREAM
                     RAGADVIYQAPLSSGGYSGIADFLVRVETASALGSFSYEVLDAKLRADTLATTILQLC
                     LYSELVAEAQGLWPKWLSVLNGAGQVERYRVEDFRAYYAEVKGLFEARAAGESHPTYP
                     EPVEHCDVCNWWSTCDKKRRLDDHLSLVPRLSRRQRAVLGRNGIDTLSALACLPPEPP
                     RLDGIARLSLSRLREQARIQVEGRGLEKPKYELLEQKPKLGLNALPEPNAGDVFLDLE
                     GDHLAVGGGLEFLFGWVTFDSKREPVYEGHWAGDAASEKRAFERLVKFLDGRDWDYPG
                     FRVYHYGAYEESALKRLMYRHGVHDREVDDFLRRNLFVDLGRIVQQSLRASVESYSLK
                     DIERLADFVREVPPREASRARKNWLLSLELGEDSTRRQADAELIRAYNREDCLALVAI
                     RDFLEQRRAELIAAGEPLERPRRPEIEMSDALAEYLRESQRLRQALLSGFPSDEASLT
                     EEQRGKRLLAGLLEFHRREDNAGAWQYFGWLRMSEQELLEDRGPIAQLSSVGVSGKVK
                     KSQSHRSSFPLQEHAIRPGTEVIDPATNKSPGDVLSVNDSEGWLELKRGGKLEQTAHP
                     SALLPNDQTVRTQELRQSLLRVAEQAIAAGLGEGSRLPVVRALLRRETQGRPPQSSDA
                     EMIAALVRSQGVFALQGPPGTGKTYSGARLIIEALRQGLRVG"
     gene            2486..>3016
                     /locus_tag="VFQ35_12545"
     CDS             2486..>3016
                     /locus_tag="VFQ35_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931518.1"
                     /translation="MRTKSFTRISRAFLGLVPLLAALIFAGCNTTDSCKTSAECQKQG
                     KCTADKNGVCVASSDADCKGSELCKAHGKCTAQTSTCTAATDDDCRGSEDCTKGGACS
                     AYQGSCTDASKAMHAECSKTCASDGLCAKQGSECVALSRFHCAGTSDEKPETESPCAR
                     LGLCGAEKGKCVATSDA"
BASE COUNT          537 a         1001 c          959 g          519 t
ORIGIN      
        1 ccccaacacg caggccctgc cgaagcgcct cgatgatgag cctcgccccc gagtaggtct
       61 tgccagtgcc cgggggccct tgcagcgcga acacaccttg cgaccgcacg agcgcggcga
      121 tcatctcggc gtcgctggac tgcggcgggc gcccttgcgt ctcgcggcgc aacagagctc
      181 gcacgactgg caaacgtgaa ccctcaccga gcccggccgc aatggcttgc tccgcaacgc
      241 gcaaaagact ctgccgcagc tcttgcgtgc gcaccgtttg gtcgttgggc aaaagcgcgc
      301 tcgggtgcgc cgtctgttcc agctttccgc ctcgcttgag ctcgagccaa ccctcgctat
      361 cgttcaccga gagcacgtcg ccgggcgact tgttggtcgc gggatcgatc acctccgttc
      421 caggccgaat ggcatgctct tggagcggaa agctggaacg gtggctttgc gacttcttca
      481 ctttgccact cacgccgacc gacgaaagct gtgcgatcgg cccccggtct tcgagcagct
      541 cttgctcgct cattcggagc cagccgaagt actgccacgc gccggcgttg tcttcgcggc
      601 gatgaaactc gaggagcccg gcgaggagac gcttgccgcg ctgctcctcg gtgagcgagg
      661 cttcgtcact cggaaacccc gacagcaagg cttgtctcag gcgctgcgac tctcgcaggt
      721 actccgcgag cgcgtcgctc atctcgatct ccggccgccg cggccgttcg aggggttcgc
      781 ccgccgcgat gagctccgcg cgacgttgct cgagaaagtc acgaatcgcg accagggcga
      841 ggcagtcttc gcggttgtag gcgcgaatga gctcggcatc ggcctgacgc cgcgtcgagt
      901 cttcaccgag ctcgagcgaa aggagccagt tctttcgcgc gcggctcgcc tcgcggggcg
      961 gaacctcacg gacgaagtcg gcgagcctct cgatgtcctt caacgaatag ctctcgaccg
     1021 acgcccgtag cgactgctgc acgatgcgtc ccaaatcgac gaacaggttc cgccgcagga
     1081 agtcgtcgac ctctcggtcg tgcacgccgt ggcgatacat gaggcgcttc aacgccgact
     1141 cttcgtacgc gccgtaatgg tagacgcgga atcccgggta gtcccagtcg cgtccgtcga
     1201 ggaacttcac caagcgctcg aaagctcgct tctcgctcgc cgcgtccccc gcccagtgcc
     1261 cctcgtacac cggttcacgc ttggaatcga acgtcaccca cccgaacagg aattcgagcc
     1321 cgccgcccac ggcgaggtgg tccccttcca aatccaagaa gacgtcgccc gcgttcggct
     1381 ccggcaacgc gttcagaccg agcttcggct tctgctcgag cagctcgtac ttcggttttt
     1441 cgagaccgcg gccttcgact tgaatgcgcg cctgctcgcg caagcgcgag agcgagagcc
     1501 gcgcgatacc gtccaatcgg ggcggctcgg gcggcagaca tgcgagagcg ctcagggtat
     1561 cgattccgtt gcggccgagc accgcgcgtt gcctgcggct gagacgcggc acgagcgaaa
     1621 ggtgatcgtc caagcgccgt ttcttgtcgc acgtcgacca ccaattgcag acgtcgcagt
     1681 gctccactgg ttcggggtac gtggggtgcg attcgcccgc cgccctcgcc tcgaacaagc
     1741 cctttacctc agcgtagtag gcgcgaaagt cttcgacccg gtatcgttcg acttgcccgg
     1801 ctccgttcaa cactgagagc cacttgggcc aaaggccttg cgcctccgcg acgagctcgg
     1861 aataaaggca cagctgaaga atggtggtcg cgagggtgtc ggctcggagc ttcgcgtcca
     1921 gcacttcgta agagaacgag ccgagagcgc ttgcggtctc tacgcgtacg aggaagtctg
     1981 caatgccgct gtatcctccg gatgagagcg gcgcttgata gatgacgtcg gcgccggcgc
     2041 gcatggcctc gcgcgtctga aagatgcctc gggcccaaaa ctgggcgtcg cgctgctcgg
     2101 cgtccgccgc gatggatacg accttgcgac ctcgagccgc gagctcgtcg aggtaacggc
     2161 gctcgtgcga gaggccgcgc aagcgcagca gctcgagcgc tggatcatcg cgaaacgtgc
     2221 gctcgagcgc gccttcgtga acgcctcgct ccaacgcgac gaggtgctcg cagctaagca
     2281 tcgcggagag atcggttggc cgatagcgcg tccagcgact cggagtggac tcggggtggt
     2341 cagccattgg ctcgacgtgt catgggtcgc gtcgacccgc aagccgcaag gaaccagagc
     2401 gcagttcagg ttcaccatcg ccagattcgg cgctttgggc taccgtgcgc gcttcgagcg
     2461 ttatccccct gcgtcggagg catccgtgag gacgaagtcg tttactcgca tcagccgggc
     2521 ttttctgggg ctggtcccgc tactcgcggc cctaattttc gcgggctgca acaccaccga
     2581 ttcgtgcaag acgtcggcgg aatgccagaa gcaaggcaag tgtaccgccg ataaaaacgg
     2641 cgtttgcgtg gctagcagcg acgccgattg caagggctcg gagctttgta aggctcacgg
     2701 aaagtgcacc gcgcaaacct cgacgtgcac tgccgccacc gacgacgact gtcgcggctc
     2761 cgaagactgc accaaaggcg gcgcgtgcag tgcctaccag ggcagctgca ccgacgccag
     2821 caaggccatg cacgccgagt gcagcaagac gtgcgcgagt gatggcctct gcgcgaagca
     2881 aggcagcgaa tgcgtggcgc tgtcacgctt tcactgcgcc gggacgagcg acgaaaagcc
     2941 ggagaccgaa agcccctgcg cgcggctcgg cctgtgtggc gccgaaaaag gcaagtgcgt
     3001 cgctacgagc gatgcc
//
LOCUS       DASTKS010000607         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1437735, whole genome shotgun sequence.
ACCESSION   DASTKS010000607 DASTKS010000000
VERSION     DASTKS010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437735"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFQ35_12550"
     CDS             complement(<1..357)
                     /locus_tag="VFQ35_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931519.1"
                     /translation="MDEPTRQRDRLHGLAVVGIAFLMSLGISFWAKRVSEPEQGAPPA
                     PPTTAGIVGWPNAVDPIKTLSVARSLTRRVLFRGMVIEGARSDGTVAASQASSRIRYV
                     FQSPAGHGPQPPRVPGV"
     gene            533..604
                     /locus_tag="VFQ35_12555"
     tRNA            533..604
                     /locus_tag="VFQ35_12555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Val,seq:cac)
     gene            700..981
                     /locus_tag="VFQ35_12560"
     CDS             700..981
                     /locus_tag="VFQ35_12560"
                     /inference="COORDINATES: protein motif:HMM:NF018045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931520.1"
                     /translation="MLFGRALKVCFACVAFVGGCAAPNATGVREVSVHEFVFGAFGGR
                     SLDLRNVCNERPARGFELRRSALDYVLSVVSIGMYVPHRIRVECEERAP"
     gene            978..1346
                     /locus_tag="VFQ35_12565"
     CDS             978..1346
                     /locus_tag="VFQ35_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931521.1"
                     /translation="MKRGLAAAWLALTCFGCASTRAFSGKPPGRTARDYDARWHSALL
                     FGGVSLSRPYDLAQICRHGWSEVRVEPDTFTFAVTLATLFIYTPSRVTVVCAAPSGAG
                     RPKLHSYPPPGIERDTLRMP"
     gene            1343..1846
                     /locus_tag="VFQ35_12570"
     CDS             1343..1846
                     /locus_tag="VFQ35_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9931522.1"
                     /translation="MKRLNVEVEGEVFETESATTGEPGAFDVLVRSAENARRAVRVLR
                     QGDDPLVLVGERVVSLRGFRERAREREVHYQGAVRRAIVTPPGAGQGRGNAAGESALT
                     SPMPGRIVAISVKPGDHVEAGSLLLVIEAMKMQNELHAKGAAVIEAVLVKPGDTVERG
                     ATLVKFG"
     gene            complement(1887..>3908)
                     /locus_tag="VFQ35_12575"
     CDS             complement(1887..>3908)
                     /locus_tag="VFQ35_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9931523.1"
                     /translation="VLGVGVGVGLSCLLPDTVRARLIGWLRPEEAEARRRRAIELGLA
                     PFAAVLGLVVLARWAVLVLASSLEAGSSGAVLSLAAPIAALLLWFGVAGAARGIAART
                     AFTSDPGRVAAVATVVATLTLLLIITSGEPSGAGGALSMLGVLTRDELDLRPLGYLAV
                     IALGAMLAPRARSIAWLAPALAGPLVLLALTARAASALDAPGAALAFERNAPLGAPLL
                     RVLRRFFDRDHDGYAVRFGGGDCDDHSAAVNPGAEDVPANGVDEDCSGSDEAPVIAAP
                     VRRPSDAVADAKALRDKLPPSLNVVLLTIDTLRYDLGYAGNPRPLSPKLDELARESVV
                     FDKAYSLASYTAKSLPPMLIGRYSSETHRGFLHFNRFEKADRFLPERLQQAGIHTVSV
                     QGHWYFFQNYGMERGFDVINSAAAPRAAQAAEGDRSVTSDKLSDEVIGELEKPELTAK
                     PFYLWAHYTDPHAEYVAHSGIDFGQGSRAAYDGEVAFVDREVGRVLDALRAKPFWGNT
                     VVIVTSDHGEAFGEHSMIRHGFELWEELVRVPLLIRVPGAPPHHVVARRSAIDLVPTA
                     LELMRVPGDAELSGVSLLPDLLADSPASRPVFVDMAEGPHNAERRAYIDGDMKLVVSG
                     GRALGLYDLAKDPAEKQDLSSDKALVSAALEQYRAFRRTLHEIYVKPTR"
BASE COUNT          664 a         1286 c         1338 g          620 t
ORIGIN      
        1 gacgccaggc acccgcggag gctgagggcc gtgtccagcc gggctctgga acacgtaacg
       61 tatccgcgag ctcgcctgcg atgccgcaac ggttccgtcg gagcgcgcgc cctcgataac
      121 catgccccgg aacaagaccc gccgcgtgag cgaccgcgcg acggacagcg tcttgatcgg
      181 atccaccgcg ttcggccaac cgacgatgcc cgccgtggta ggcggcgcag gcggagcccc
      241 ctgctcgggt tcggagacgc gcttggccca gaacgaaatt ccaaggctca tcaggaaagc
      301 aattccgacc acggcaagcc cgtgaagccg gtcgcgttgg cgcgtgggct catccatccg
      361 agagacgtcc gtagtgggcg ttgagggctg aagacgacac gggccgtccc tataacatcc
      421 tgcgctccgt cttgggagtc gtcccgagcg gtctcaaagc cccgtctgcg cgattcttta
      481 acagaaacga ttgcgggggc gccgagagcg cgctagaagg gctgccgctt tcgggcgcat
      541 aactcagcgg tagagtgcgt ccttcacacg gacgaagtcg caggttcgat ccctgctgcg
      601 cccacgaaga aatcccggaa gcccggcctg ctccttcaag cagcggccgg cgttgctgtt
      661 tcaattcgcc cgagcgcgct cgggtgtaac gtcggtcctg tgctgttcgg ccgcgcgctg
      721 aaagtctgct tcgcctgcgt cgcattcgtt ggtggatgcg ccgcgccgaa cgcgacgggc
      781 gtgcgtgaag tctccgtgca cgagttcgtg ttcggcgcat tcggtgggcg ttcgctcgat
      841 ttgcgcaatg tgtgcaacga gcggcctgct cgggggttcg agctacgccg ctcggcgctc
      901 gactacgtgc tgagcgtggt ctcgatcggt atgtacgtcc cccaccgaat tcgcgtggaa
      961 tgcgaggagc gggcgccgtg aagcgcggac tagccgccgc ctggctcgcg ctcacgtgct
     1021 tcgggtgtgc gagcactcgc gcgttttccg gcaagcctcc cggcaggacc gcgcgcgact
     1081 acgatgctcg ttggcactcc gcgctgctct tcgggggagt ttcgctctcg agaccttacg
     1141 acctcgcgca gatctgccgg cacggctggt cggaagtgcg cgtcgagccg gacacgttca
     1201 cgttcgcggt gacgctggcc acgttgttca tctacacgcc gtcgcgcgtc accgtagtct
     1261 gcgctgcgcc gagcggcgcc ggaaggccca agttgcactc atacccaccg cccggaatcg
     1321 agcgtgatac gctgcgcatg ccatgaaacg cctcaacgta gaggtcgagg gcgaggtctt
     1381 cgaaaccgaa tccgctacga cgggcgagcc cggcgcgttc gacgtgctgg ttcgaagcgc
     1441 cgaaaacgcg cgccgcgcgg tgcgtgtgct tcggcagggc gacgacccgc tggtgttggt
     1501 aggagagcgc gtcgtttcgt tgcgtggctt ccgcgagcgc gctcgcgagc gcgaagtcca
     1561 ctatcagggc gcggtgcggc gggcgatcgt cacgccgccc ggcgctgggc aaggcagggg
     1621 caacgctgcc ggcgagagcg ccctcacctc gcccatgcca ggtcgcatcg tcgccatatc
     1681 ggtgaagccc ggtgatcacg tcgaagcggg ttccctgttg ctcgtgatcg aagcgatgaa
     1741 gatgcaaaac gagctgcacg caaaaggcgc ggcggtcatc gaagccgtgt tggtcaaacc
     1801 gggcgacacg gtcgagcgcg gcgcgacgtt ggtgaagttc ggctgacgcc tgaagcgact
     1861 cgtccgggta ctgccgcacg ccgagctcag cgtgttggct tgacgtagat ctcgtgcaac
     1921 gtgcgacgga aggcgcggta ctgctcgagc gccgcagaga cgagcgcctt gtcgctcgag
     1981 agatcctgct tctcagcggg gtccttggcg agatcgtaga gtccgagcgc gcgcccgccg
     2041 ctcacgacga gcttcatgtc cccgtcgatg tacgcgcgcc gttcagcgtt gtgaggcccc
     2101 tcggccatat cgacgaacac gggtcgcgag gcgggtgagt cggcgagcag gtctggcaag
     2161 agagagaccc cgctcagctc cgcgtcgccc ggtacgcgca tgagctcgag cgcggttgga
     2221 accagatcga tcgcgctccg gcgtgcaacg acgtggtgcg gcggcgcgcc cggtacccgg
     2281 atcaaaagcg gcacgcgcac gagctcttcc cagagctcga agccatggcg aatcatcgag
     2341 tgttcaccga acgcctcgcc gtgatcgctg gtcacgatga ccaccgtgtt gccccagaac
     2401 ggcttggctc tcagggcgtc gagcacccgc cccacctctc gatcgacgaa agcaacttcg
     2461 ccgtcgtagg ccgcgcggct gccctgaccg aagtcgatgc cggaatgcgc gacgtactcg
     2521 gcgtgaggat ccgtatagtg cgcccagaga tagaatggct tggcggtgag ctccggcttc
     2581 tcgagctcac cgatgacttc gtcgctgagc ttgtcgctcg tgacgctgcg gtcgccctcc
     2641 gcggcttgtg cggcgcgcgg cgcggcggcg ctgttgatga cgtcgaagcc gcgttccatc
     2701 ccgtaattct ggaagaagta ccagtgccct tgaacgctca cggtgtggat gcccgcctgc
     2761 tgcaggcgct cgggcagaaa gcgatcggcc ttctcgaagc ggttgaaatg caaaaaccca
     2821 cggtgggtct cgctcgagta tctaccgatc agcatcggcg ggaggctctt cgcggtgtac
     2881 gaggcgagcg agtacgcttt gtcgaacacg acgctctcgc gagcgagctc gtcgagcttg
     2941 ggtgacagcg gtcgggggtt gccggcgtag ccgagatcgt atctgagcgt atcgatcgtc
     3001 agcagcacca cgttgagcga cgggggcaac ttgtcgcgca acgccttggc gtccgccacc
     3061 gcgtctgatg gcctgcgcac cggagcagcg atgactgggg cttcgtcgct gcccgagcaa
     3121 tcttcgtcga cgccgttcgc cggaacgtcc tctgcgcccg ggtttacggc ggcggagtga
     3181 tcgtcgcagt caccaccccc gaagcgaacg gcgtagccgt cgtgatctcg gtcgaagaag
     3241 cgccgcaaga cgcgcagcag cggagcgccg aggggcgcgt tgcgctcgaa ggcgagcgcc
     3301 gctccgggcg cgtcgagagc gctcgcggcg cgggccgtga gagccaagag cacgagcgga
     3361 cccgcgagag ccggcgcgag ccacgcgatg gaacgagccc gaggagcgag catcgccccg
     3421 agcgcaatca cggcgaggta gccgagcggg cgcagatcga gctcgtcgcg cgtcaacacg
     3481 ccgagcatcg agagcgctcc tccggcgccg ctcggttccc cgctcgtgat gatgagcaac
     3541 aacgtgagcg tcgccacgac cgtagcgacg gccgcgacac gaccggggtc cgaagtgaaa
     3601 gcggtgcgag ccgcgatacc tcgcgccgca cctgcgaccc cgaaccagag aaggagcgcg
     3661 gcaatcggag ccgcgaggga gagcaccgcg ccgctcgacc ctgcctcgag tgacgacgcg
     3721 agcaccagca cggcccagcg cgcgagcacg acgagcccga gcaccgccgc aaacggcgcg
     3781 agcccgagct ctatcgcgcg ccggcgtctc gcttccgcct cctcggggcg aagccagccg
     3841 attagccgcg cgcgcacggt gtcaggcaac aagcacgaga gcccgacgcc aaccccgacg
     3901 ccgagcac
//
LOCUS       DASTKS010000608         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1213747, whole genome shotgun sequence.
ACCESSION   DASTKS010000608 DASTKS010000000
VERSION     DASTKS010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213747"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFQ35_12580"
     CDS             <1..210
                     /locus_tag="VFQ35_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931524.1"
                     /translation="FFSIQSGVRFVVALVPPLVFVGAWLSDSSRRLNFRRYIGFFALV
                     ALAWLALDLPELLSTVVVKARNAGW"
     gene            complement(299..775)
                     /locus_tag="VFQ35_12585"
     CDS             complement(299..775)
                     /locus_tag="VFQ35_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931525.1"
                     /translation="MDFTFRRGTNGDKTYFVLLHSEATPEEARWNAYLDAISRALAGS
                     VGRTHVFVATDGGGPDPAQRKALARVFEQGDALTHVFTTSVVVRGIVTAFRWIAGTRA
                     VAHQPQDFGRTCVEVGHDPALVVQSFDEAQSELAPVALLNELRRSALVPARLQIIR"
     gene            1050..1361
                     /locus_tag="VFQ35_12590"
     CDS             1050..1361
                     /locus_tag="VFQ35_12590"
                     /inference="COORDINATES: protein motif:HMM:NF018563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HET9931526.1"
                     /translation="MKFENVTATAKANVYFDGKVVSHTITFADGSKKTLGLIYAGEFH
                     FGTDAAERMDITAGLCRVRVDGTSEWKEYGAGSFFRVPAKSGFDIAVSSGIAEYVCSF
                     E"
     gene            complement(1377..>2536)
                     /locus_tag="VFQ35_12595"
     CDS             complement(1377..>2536)
                     /locus_tag="VFQ35_12595"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931527.1"
                     /translation="KLGAVKAPRHLQCSHRPACPGCPRFGVPGLAPSARATLAKLAER
                     AGLPEPALHEGAALDFRVRARLAVRGRAGQPKIGIFQLGTHRVVHIPQCRIHHPVINE
                     VSEQVRQALAAHRLPLYSDSAHAGRVRYLQIVVQRQSGSAQLVVVTNDPAYSGLDAFF
                     SDVAERLGSRLHSLFWNGQPERSNAVLGGAWHRFAGPQALEESFAGVRLFYPPDAFGQ
                     SHLNLAERISERVSVFVPQGARVVEFYAGVGALGLPLVPRIESLTLNEIGEGSLRGLS
                     LGIEALPEVVRPRVCVVPGAAGDAARLVTEDVDVVIVDPPRKGLDEPLLSRLVENPPR
                     RLIYVSCGLDALVRETESLLSRGAFALSALEAFALFPYTEHVETLAVFDRV"
BASE COUNT          462 a          842 c          818 g          414 t
ORIGIN      
        1 ttcttttcga tccagagcgg cgttcgcttc gtcgtcgcgt tggtgccccc gctcgtgttc
       61 gtcggcgctt ggctctccga ctcgagtcgc cgcctcaatt ttcgacgata catcggcttc
      121 ttcgcgctcg tcgcgcttgc gtggctcgcg ctcgacctcc ctgaattgct ctcgaccgtg
      181 gtcgtcaaag cgcgcaacgc tggctggtag gtcccacgcc ggcccacgcc accccagcgc
      241 ccgtcatgtc gtcgccgcag acccttcggg cctgcacaac tccgtcgcac gccgatgctc
      301 agcgaattat ctgcaggcgc gccggaacca acgcgctacg gcgaagctcg ttcaataggg
      361 caaccggcgc gagctccgac tgcgcctcgt cgaacgactg gactacgagc gccggatcgt
      421 gcccgacttc gacgcaagtc ctgccaaaat cttgcggctg gtgcgccacc gcccgagtac
      481 ccgctatcca gcggaacgca gtcacgattc cgcgcacgac gacgctcgtc gtaaatacgt
      541 gggtgagcgc atcgccttgc tcgaacactc gcgcaagcgc tttgcgctgc gctggatccg
      601 ggccgccacc gtcggtagcg acgaacacgt gcgtgcgacc gacacttccg gcgagagctc
      661 gcgagatcgc gtcgaggtac gcgttccagc gcgcctcctc cggcgtcgcc tcactgtgaa
      721 gcagcacgaa gtacgtcttg tccccgttcg tcccgcggcg gaatgtgaag tccactacga
      781 acttctatat cagccagaat cgaagaaact atagtggctt cgtccgtaaa aatcgcgtca
      841 cgctccgaca gaggctcgca cattgaaagc ggccgacaca cttgcatcca cggttcctta
      901 cggcgcgtcc gttttcgcct ggctcgcgcg cggcgagggc gtagcgcgtc gtgtttcggc
      961 aacgctcctc gccgagcgct ggcggacacc gcttgccggg gcaaacgccg ctttacatcg
     1021 agtccgggca ctactatcgg gggagcgcca tgaaattcga gaacgtcacc gccaccgcaa
     1081 aagccaacgt gtacttcgac ggcaaggtcg tgtcgcacac gatcaccttc gctgacggct
     1141 cgaaaaagac gctcggcctc atctatgcag gtgagtttca cttcggcacc gacgcggctg
     1201 aacgcatgga catcacggcc gggctttgcc gagtgcgcgt cgatggcacg agcgagtgga
     1261 aagagtacgg agcgggctcg ttctttcgcg tcccggccaa gtccgggttc gacatcgcag
     1321 tctcgagcgg catcgccgag tacgtctgct cgttcgagtg agccgcgcga gcgtcgttac
     1381 acgcggtcga aaacggcgag cgtctcgacg tgctcggtgt atggaaagag cgcgaacgct
     1441 tcgagagcgc tgagcgcgaa ggcgccccgc gagagcaagc tctcggtctc gcgcacgaga
     1501 gcgtcgaggc cgcagctcac gtagatgagg cgccgaggag ggttttcgac gagacgcgac
     1561 agcagcggct cgtcgagccc cttgcggggc ggatcgacga tgaccacgtc gacgtcctcc
     1621 gtgacgagtc gcgcggcgtc gcctgctgct ccgggcacga cgcaaacacg gggacgcacg
     1681 acttcgggca aagcttcgat accgagcgaa agaccccgca acgagccctc cccgatttcg
     1741 ttcagcgtaa gcgactcgat gcgcgggacg agcggcaacc ccaacgcgcc aaccccggcg
     1801 tagaactcga cgacgcgcgc gccctgcggc acgaagaccg agacccgctc cgaaatacgc
     1861 tcggcaaggt tcaggtggct ctggccgaaa gcgtcgggcg gataaaagag gcgaacgccg
     1921 gcgaacgact cctcgagagc ttggggcccc gcgaaccggt gccatgcgcc gccgagcacg
     1981 gcgttgctgc gctcgggttg gccattccaa aacaggctgt gcagcctcga accgagccgc
     2041 tcggcgacat ccgagaagaa agcgtcgagg ccactgtagg ccgggtcgtt ggtcacgacc
     2101 acgagctgcg ccgagccgct ttggcgctga acgacgatct gcaggtaccg gactcgaccg
     2161 gcgtgggcac tatcggagta aagtggcagc cgatgcgccg ccagggcctg acgaacctgt
     2221 tcgctgacct cgttgatgac cgggtggtga atgcggcact gcggaatgtg tacgacccga
     2281 tgcgtgccga gctgaaagat cccgatcttg ggttggcccg cgcggccgcg cacggcgagt
     2341 cgagcgcgca cgcgaaagtc gagcgcagcg ccttcgtgca gcgcgggctc gggcaagccg
     2401 gcgcgctccg caagcttcgc gagcgttgcg cgagcgctcg gcgcgaggcc gggaacacca
     2461 aagcgcgggc accctgggca cgcggggcga tgagagcact gaaggtgtcg gggcgccttc
     2521 acggccccga gcttga
//
LOCUS       DASTKS010000609         5928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1659977, whole genome shotgun sequence.
ACCESSION   DASTKS010000609 DASTKS010000000
VERSION     DASTKS010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659977"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFQ35_12600"
     CDS             complement(<1..848)
                     /locus_tag="VFQ35_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9931528.1"
                     /translation="MNPSARPALLTLRPIQSAAAGLSVACAVLASGCEQASSSANEGY
                     RRAQPSATKLSPLGTHCPPPSPPVEPDRGDGAEALARINHFVILYMENHSFDSLYGLF
                     PGADGLLDASCALTTPRQVDKFGVEYPMLDAVPSAPPGMAENLPNQPFLMDARFPPNV
                     PPPDLNHSFYGQQAQLNGGAMNQFAAFSDARGLTVAYYDTAKLPLAKLAEKYTLCDRF
                     FQSAFGGSFLNHQWLIAARTPYAPTLVDPNREPDLNDISKPFPVDGATGIFTGADPAI
                     TSDGFAV"
     gene            complement(1026..1688)
                     /locus_tag="VFQ35_12605"
     CDS             complement(1026..1688)
                     /locus_tag="VFQ35_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9931529.1"
                     /translation="MNLDSFRATATPVDLAEVWARLAARELVVLASGATDDSASLTIK
                     AAARLEFDESLERSLNILRRVLLGETQKAVAANCSIAASTVAMHCSRGLTRMGAPKLT
                     TRTPLVLVIAAHASAGFAFPFVSFENVEDGALRIAVRLAGPQVVPHLSGVERDILRGV
                     TCGLDNTEIATKRGTSRRTIANQLGSLFRKFGVSGRGELLAKIARSLALHTPNVLHEP
                     QG"
     gene            2080..2799
                     /locus_tag="VFQ35_12610"
     CDS             2080..2799
                     /locus_tag="VFQ35_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HET9931530.1"
                     /translation="MFRAIIVEDEAASRDRLRRLLEQHHGDEVEIVDEADSGPAAVAT
                     INERRPDLVFLDVSLPGFDGFDVLRRITSAVKVVFTTAYSEYAVQAFRERALHYLLKP
                     IDPEQLSEALDRVRASSKKESSEPARTALNRILCRDRDTTYVIRPSDVLFLKADQGYT
                     LVRTQNKEYLTADALASLEEALGDDFVRIHRNAVVNVTHVASLKHTGGEVTIVLQNGL
                     ELPVSRRHVPGLREKLLYGAD"
     gene            2890..4407
                     /locus_tag="VFQ35_12615"
     CDS             2890..4407
                     /locus_tag="VFQ35_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931531.1"
                     /translation="MTLDPSSLFNDLRDDGGAPVDGQQLLADLQGNILRGYGKPRTLL
                     VFVRFGPDPATNRRWVRDHVLRSEPGLRPASEQLSSAPVQTTFSLSASGLARLGCPSP
                     KLERFSGAFVRGARAEATRRRLDDPPVDAWERAFQKPWDALWLLACDVGALEVQRSII
                     EHAVRAEAISAALIESGNVLDRQGTPVGPDWTTEQPRFEPFGYRDGISTPVFSQAHLD
                     ELALSGKAITHQKRNLSVVLARDPLSSSGFGSYVVFRKLFQDVAGFRRRLDDMVRKLR
                     WRGPELSRVWGTDSSMPYGAFVDGSGAFDDSKLEDFVRQRIMGRSADGTPATLGPSAN
                     NPTNDFDYAEDDGRRCPFSAHVRKVCPRGGYSDLASEATHSVVRRGVSFGRADSEADP
                     ERGAGLLFMCAQSDIENQFEFVQARWVNDAERDFSAEPLPMRDNIAASGGASRPNGYV
                     QDPESRDDRGVRYAWLTETMEFDAKMYDLVSLRGAEYLFTPSISGLRALCQGGAP"
     gene            4404..5138
                     /locus_tag="VFQ35_12620"
     CDS             4404..5138
                     /locus_tag="VFQ35_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931532.1"
                     /translation="MSQRKPRGLLSFIRNTTGVAAYRDEMHVDMRAAFERFGITDGAV
                     QAVCFDVERAAYEIRRSSDDRFGRHASPFAASRHSAARKLMAESSLALFRELSNDLSV
                     RLLAREELAPGLDLAHEVRKDAPPSGVVRGEGPIELHAERIEVVAERELGEAPLLLDI
                     VYYFFHFPSTRAPLLSSHALERAVDAIAQKSGERNLYAQVHEGLTALDHYLRAGEPAS
                     RVSSLVERLCELLAVEYDDRASCYCW"
     gene            5142..>5928
                     /locus_tag="VFQ35_12625"
     CDS             5142..>5928
                     /locus_tag="VFQ35_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931533.1"
                     /translation="MRWSSSAVFREVVAKVPRALASSRRLELCHTVAASVPPAAAAYF
                     ECRLNEDERVDYLLLTRDRLDAADRFKRARGGLSDSSVWEKNRGLLQAWSDNVGNLAD
                     IPLLWFEYDIDERFDERVMDASPSLCVERGYHRREIRAPSLEPRRARRLAESALERLW
                     SGDAVSIVAALRCIDALPERGALVYVSSMLTRPECVTKVYVSLPKTSVFDYLRRISWP
                     GDPSLLEQVLRGWYAPIAETVFIDVTVSDRVHARLGLALSQLHR"
BASE COUNT         1028 a         1860 c         1945 g         1095 t
ORIGIN      
        1 accgcaaagc cgtcggacgt gatcgcggga tcggccccag tgaagatgcc ggtcgccccg
       61 tcgacgggaa aaggcttgct gatgtcgttg aggtcgggct cccggttcgg gtcgaccaac
      121 gtcggagcgt aaggcgtgcg cgcggcgatg agccactggt gattcaagaa cgagccgccg
      181 aacgcggatt gaaagaagcg atcgcagagc gtgtacttct ccgcgagctt cgcgagcggc
      241 agcttggccg tgtcgtagta cgcgacggtc aaccccctgg catcgctgaa ggccgcgaac
      301 tggttcatcg ccccgccgtt gagttgcgct tgctgcccat agaacgaatg gttcaaatcg
      361 ggcggcggaa cgttgggtgg gaaccgcgcg tccatcaaga acggttggtt cgggaggttc
      421 tcggccatgc cgggaggagc gctcggtacg gcgtccagca tcgggtactc gaccccgaat
      481 ttgtccacct gcctcggcgt ggtgagcgcg cagctagcgt cgagcagccc gtccgccccg
      541 ggaaacaagc cgtaaaggct gtcgaagctg tgattctcca tgtagaggat cacgaagtga
      601 ttgatgcgcg cgagcgcctc tgcgccgtcc ccgcgatcgg gctctacggg cggcgacggt
      661 ggcgggcagt gcgtgccgag cgggctcagc ttggtcgcag acggctgcgc gcggcgatag
      721 ccttcatttg ccgaggagga ggcttgctcg cagccgctcg ccagcacggc gcacgcgacg
      781 gatagcccgg cggctgcgct ctggatcggt cgtagggtaa ggagcgcagg gcgcgcgctc
      841 gggttcatga tgctccctca gtcgtaaggc gccgtcggca acggctcgcc ccatactagt
      901 cacgcgaagc gtcgcgcgcc cgtcagacaa tgcagacggc tgccgactcc gagcactcgc
      961 ggacggaaaa atccgctcga ggggaagcta gcgagcgtcg agctcgaacc tagcgcagca
     1021 acgtcctacc cttggggttc atgtaggaca ttgggcgtgt gcaacgcgag actccgcgcg
     1081 atctttgcga gcagctctcc ccgaccggaa actccgaact ttctgaacag cgagccgagt
     1141 tggttcgcga tcgttcggcg tgacgtgccg cgcttggtcg cgatctcggt gttgtcgagt
     1201 ccgcacgtca cgccccgcaa gatgtcgcgc tcgacgcccg acaagtgcgg aacgacctgt
     1261 ggccccgcca accgaaccgc aatccggagc gctccgtcct cgacgttctc gaaagaaacg
     1321 aacggaaagg cgaagcctgc gctcgcgtgc gccgctatca cgagtacgag cggcgttctc
     1381 gtcgtcagct tgggggcgcc catccgcgtc aggccgcgcg agcaatgcat cgcgaccgta
     1441 gacgctgcga tcgagcaatt ggccgcgacc gccttctgcg tctctccaag caagacccgg
     1501 cgcagaatgt tcaagctgcg ctccagggac tcgtcgaact cgagccgagc cgccgccttg
     1561 atcgtaagtg acgccgagtc gtcggtcgct cccgatgcca gcaccacgag ttcgcgcgca
     1621 gcgagccgag cccagacctc tgccaggtcg acgggcgtgg ccgtcgctcg aaaggagtcg
     1681 aggttcatcg actcgaggtc cagcgctggt ccgagtatcc ctcttccgcg acgctgttcg
     1741 gaactccagg taacgccttg cacggctctc cctcgctcta acgaaagtcc ggcgaacgcc
     1801 ttgttatcga gaacctcgcc aacggttcca atccgaatcc cgtgaacggc gccttcgggt
     1861 ccgcgagcgg tgacgtccgg aacatgagtg gtcgcggcga gccgcttccg gggagctagg
     1921 cgcgcccgtc cggcgagttt ctcggcgatg tcgaggtccc gttcggaggg tcggtaaagg
     1981 cagcggggcg gcggagtggg gccggtctcc tcaggaaagt ccgtcgattc cggccactgg
     2041 ttgcgactcg agttcggact tgctacctcg aaagattgaa tgttcagggc gatcatcgtc
     2101 gaagatgaag ccgcgtctcg cgatcgcctg cgtcgactcc tggagcagca ccatggggat
     2161 gaagtcgaga tagtcgatga ggccgacagc ggccccgctg cggttgcgac cataaacgaa
     2221 cgacggcccg atctcgtctt cttggacgta tctctcccgg ggttcgacgg cttcgatgtg
     2281 ctgcgccgta ttacttcggc ggtcaaggtg gtcttcacga ccgcatactc ggagtacgcc
     2341 gtgcaggcgt ttcgggagcg cgcgctgcat tacctcctga agccgatcga tccggagcag
     2401 ttgagtgagg cactcgatcg cgtgcgcgcg agctcgaaga aggagtcgag cgagcctgcc
     2461 cgcaccgctc tcaatcggat tctgtgtcgc gatcgcgata ccacatacgt aatcaggccg
     2521 agtgacgtgc tctttctcaa ggcggaccag ggctacacgc tggtccgcac gcagaacaag
     2581 gagtacctga ccgccgacgc tctcgcgtcg ctggaagagg cgctcggcga cgatttcgtg
     2641 cgcatccacc gaaacgcggt ggtgaacgtc acgcacgtcg cttcactcaa acacacgggc
     2701 ggtgaagtca cgatcgtgct tcaaaacggg ctcgagttgc cagtgagtcg ccgtcacgta
     2761 ccagggttga gagagaagct tttatacggc gccgattgac gtcgtaaatt gcccgctcga
     2821 aggcgagtta ctaccgttcg atgggttcgc gcgcacgata tcctcacaca gttgtagaag
     2881 cgaatgagga tgacactcga tccaagttcg ctcttcaatg atctgcgaga cgatggcggt
     2941 gcacccgtgg acggccagca gctactcgcg gacctccaag gcaacatcct gaggggctac
     3001 ggcaagccgc gtacgctgct cgtttttgta cgcttcgggc cggacccagc taccaatcgt
     3061 cgttgggtac gggaccacgt gcttcgctcg gagcctggct tgaggcccgc gtcggagcag
     3121 ctttcgagcg ctccggttca gaccaccttc tcgctcagcg cttcggggct cgcgcggctc
     3181 ggttgtccca gtccgaagct cgagcgtttc agcggcgctt tcgtgcgcgg cgcacgcgcc
     3241 gaggcgacgc ggcggcggct cgatgatccg cccgtcgacg cctgggaaag agcttttcaa
     3301 aagccgtggg acgcgctgtg gctcttggcg tgcgacgtcg gtgcgctgga ggtgcagcgc
     3361 tcgatcatcg agcacgcggt gcgcgcggag gcgatttcgg cggcgctgat cgagagcggc
     3421 aacgtgctgg atcgacaggg cacgccggtg gggcccgatt ggacgacgga gcagcctcgg
     3481 ttcgagccat tcggctatcg cgacggcatc agcactcccg tcttcagcca agcgcatttg
     3541 gacgagctcg cgctcagcgg gaaggcgatt acccaccaaa agcgcaattt gtcggtcgtc
     3601 ctggcgcgcg atcctctctc gagttctggg tttggctcct acgtcgtttt tcgcaagttg
     3661 ttccaagacg tagcggggtt tcgccgtcgc ctcgacgaca tggttcgaaa gctacgctgg
     3721 cgaggccccg agctgagccg ggtgtggggc acggactcga gcatgccgta cggcgcgttc
     3781 gtcgacggct ccggcgcgtt cgacgattcg aagctcgaag acttcgttcg ccagcgcatc
     3841 atgggccgct ctgccgacgg caccccggcg acgctcggcc catccgccaa caatccgacc
     3901 aacgacttcg attacgcgga ggacgacggg cgtcgctgtc ccttttcggc ccacgttcgc
     3961 aaggtgtgcc cacggggcgg ctactcggat ctcgcgagcg aggcgaccca cagcgtcgtt
     4021 cggcgtggcg tttctttcgg tcgcgccgac agcgaggctg acccggagcg cggtgcgggg
     4081 ctgctcttca tgtgcgcgca gagcgacatc gaaaatcaat tcgagttcgt tcaggcgcgt
     4141 tgggtgaacg acgccgagcg cgatttcagt gccgagccat tgccgatgcg cgacaacatc
     4201 gcggcgagcg ggggcgcgag tcggcccaac ggatacgtgc aagacccgga gagtcgcgac
     4261 gaccgcggcg tccgctacgc ctggctcacc gagaccatgg agttcgacgc caagatgtac
     4321 gacctcgtca gcttgcgcgg cgcggaatac ctgtttacgc cgagcatctc ggggctccgc
     4381 gcgctctgcc aaggaggtgc gccgtgagtc agcgcaagcc gagagggctg ctctcgttca
     4441 ttcgaaatac gaccggcgtc gccgcctatc gcgacgaaat gcacgtcgat atgcgcgcgg
     4501 ccttcgaacg cttcggcatc accgacggtg cggtgcaggc agtgtgtttc gacgtcgagc
     4561 gagccgcgta cgaaattcgt cgcagttcgg acgatcgatt cgggcgacat gcttcgccat
     4621 ttgcggcgtc gcgtcactcc gcggcgcgca agctgatggc tgagtccagc ctcgctctgt
     4681 ttcgcgaact ttcgaacgac ctatcggtgc gtttgctggc gcgcgaagag ctcgcgccgg
     4741 ggctcgactt ggcccacgaa gttcgcaaag acgcgccccc ttctggcgta gttcgcggcg
     4801 agggaccgat cgagctccac gccgagcgca tcgaagtcgt cgccgagcgg gagctcggtg
     4861 aagcgccgtt gctgctcgac atcgtttact acttctttca ttttccgagc acgcgcgccc
     4921 cgctcctctc gagccacgcc ctcgagcgcg ccgtcgacgc catcgctcaa aaaagcggcg
     4981 aacggaacct gtacgctcaa gttcacgagg gccttaccgc cctcgaccac tacctgcggg
     5041 cgggcgagcc tgcctcacgc gtgagctcgc tcgtcgagcg gctctgcgaa ctattggcgg
     5101 tcgagtatga cgaccgcgcg tcttgctact gctggtgagc cgtgcgttgg tcgagttccg
     5161 ccgtgtttcg agaggtggtc gccaaagtgc cacgcgcgtt ggcgtcctct cgccgtctcg
     5221 aactttgtca caccgtcgcg gccagcgtgc cgcccgctgc ggccgcatac ttcgagtgtc
     5281 gcctgaacga ggacgaacgg gtcgactatc tcctcctgac gcgcgacagg ctcgacgcgg
     5341 ccgatcgatt caaaagggct cggggcgggc tctccgactc gtcggtatgg gaaaaaaatc
     5401 ggggactgct ccaggcttgg tcggacaacg tcgggaactt ggcggacatc ccgctgctct
     5461 ggttcgagta cgacatcgac gaacgtttcg acgaaagagt gatggacgcg agcccgtcgc
     5521 tttgcgtcga aaggggctat catcgccgcg aaatcagggc gccgagcctc gagccacggc
     5581 gtgctaggcg tttggcagag tctgcgctcg agcgcctgtg gagcggagac gcagtgtcca
     5641 ttgtcgcagc tctgcggtgc atcgacgcgc tgccggagcg aggcgcattg gtctacgttt
     5701 cgagcatgct cacgcgcccg gagtgtgtga cgaaggtcta cgtttcgctc cccaagactt
     5761 cggtttttga ctatcttcgg cgcatctcat ggccgggaga tccgtcgctg ctcgagcagg
     5821 tgcttcgcgg ttggtacgcg ccaatcgccg aaacggtgtt catcgacgtc acggtcagcg
     5881 accgagtgca cgcaaggttg ggcctcgcct tgtcccaact gcaccgac
//
LOCUS       DASTKS010000610         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1438016, whole genome shotgun sequence.
ACCESSION   DASTKS010000610 DASTKS010000000
VERSION     DASTKS010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438016"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /locus_tag="VFQ35_12630"
     CDS             complement(<1..1982)
                     /locus_tag="VFQ35_12630"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9931534.1"
                     /translation="MGSPAKNQKFALDEELWFGDHDDADAENRAAASLAAVGARIVGA
                     KPFPVAARRLDELTRNPSTRIEQVVNVLESDPALSARLLRLVNSAGYGLKVRCTSVRH
                     AAVLVGTRRLNQVATTAAILDLFDGNSTHAMELLEHAAIVGSLCRYLAVHQGLPHDEL
                     FTCGFLHDIGKLMLLDAERDRYAELLNEFGHAPDQIHLAERRLFGFDHAVLGAHVLTA
                     WNIPEPVPRVIAWHHAPARAMQDTVVSAMVQTLRLADLLAYVLALPDTEQAIQLGAAS
                     EAANYLEISDVQLSAMWNDLVALRDRSRARSHGEPELEVIVPKVEVPSLRARASTTPS
                     QRPAKTGRPLHEVPSHFPCSVCAKPSFGNVCAACGGHVCPAHQVGADEWCSVCAREYV
                     RFKKTAELSGGLRAGVGTLVGSTLAIALYSASRAPDAGVETLILAPVMVLALWAVVLP
                     VAYRLWQKLAFLERRRKARLSEPRASVARPRVAHMPSAASASALIVASPVEATPQPAA
                     ATTSTSELDANGEHDTPLSMAPLSTAPSVPPALSPALSSPPRSSRSRIALEPPVEPSA
                     PESVAPPAAQRAPETGPEARNPSMLAESLLPDSEFLSVRPTTVSIALAAHFGKNGSQR
                     PSARSKAPHADEDIRSIHPERSSLVSERPRLAVSDS"
     gene            complement(2001..2471)
                     /gene="fliW"
                     /locus_tag="VFQ35_12635"
     CDS             complement(2001..2471)
                     /gene="fliW"
                     /locus_tag="VFQ35_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HET9931535.1"
                     /translation="MNITSQRFGALEIEEEQVLRFPRGVIGFPHEREFVLVPHNATGF
                     LAWLQSAKTPELALPVVSAHAFGAKYPDVDMEPVISDANLGGVEEDLALMVVLCAPQG
                     QPATVNLLAPIIVNAATRCGAQVILEGSRFSTRELFVREPEAEGEAPKAAAAAE"
     gene            complement(2632..3372)
                     /gene="fliA"
                     /locus_tag="VFQ35_12640"
     CDS             complement(2632..3372)
                     /gene="fliA"
                     /locus_tag="VFQ35_12640"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HET9931536.1"
                     /translation="METVSVSGTPATRMVRDRTQDRQVYERYMPLVRRIAMRTIRTLP
                     SSITFDDILSAGWVGMAEALQRRAEGMEDEHFEAYASYRVRGAILDYLRALDPLSRKL
                     RGASRRITTAASDLTQRLGRMPTEEEVAGELGLSLSAYQDLLGEISDAGFARLELGSI
                     AEPPTLDPSPEATTVRAELQSTVVAAIEELPERLRLVLSLHYQEECSLREIGEVLGVT
                     ESRVCQLHSEAVHLIRARVEGSVVRRRR"
     gene            complement(3378..4499)
                     /gene="flhF"
                     /locus_tag="VFQ35_12645"
     CDS             complement(3378..4499)
                     /gene="flhF"
                     /locus_tag="VFQ35_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HET9931537.1"
                     /translation="MQYQSFRGADLREALGQVKQTLGPNALIETTRQVTNGQAGGLER
                     SYVEVTAAAPAGIDWPFAAKAGIRTNANRPPAGAQGQKQGARGQSFQLLGGAHPDVER
                     ELSNLRAMVEELNANLPPRERALAMLHARGIEGALARELANGAARPAKKGREALQNWL
                     VERVRERLDVAEDLLNVEGPMILACVGPTGVGKTTTLAKLAARARLDLGRSVAVVSLD
                     SFRVGAAEQWQRYASLMGIPLHLVHEAAGFRRALRDTRADIVLVDTAGRSAHSVGSVL
                     AESLRGITQVRVNVALVVPAWLRANDAERVVEGYSDPALTHVIVTKLDETQQIGGALH
                     ASMPRRLPLAYLCNGPRVPEDITDASRDSVLAALFPSDD"
     gene            complement(4545..>4890)
                     /locus_tag="VFQ35_12650"
     CDS             complement(4545..>4890)
                     /locus_tag="VFQ35_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HET9931538.1"
                     /translation="AAFTGHDGTLSALVLDAPVEDMFRRSLRDIAAGTGGALDPEQAQ
                     RLGVSLEAAVKRMMQAGRAPCVITSPDVRRYLRAFAERRCPELAVLSFRELEPDATIR
                     PFETIGFGKAAA"
BASE COUNT          846 a         1587 c         1652 g          805 t
ORIGIN      
        1 atggagtcgc tgacggccag tcgaggacgc tcgctcacca gagacgagcg ctctggatgt
       61 atcgagcgaa tgtcttcgtc tgcgtgagga gctttggagc gtgcgctcgg acgctgcgaa
      121 ccgttctttc cgaagtgggc ggcgagcgcg atcgagacgg tcgtcggccg cacggaaagg
      181 aactcggagt ccggaagcaa cgactcggcg agcatggaag gattgcgcgc ctctggcccg
      241 gtttcgggcg ctcgctgagc cgcgggtggc gcaaccgatt ccggtgcgct gggctcgacg
      301 ggcggctcga gtgcgatccg cgagcggctc gatcgcggag ggctcgaaag cgcaggcgac
      361 agagccggcg gcaccgaggg cgcggtcgaa agcggtgcca tcgaaagcgg agtgtcatgc
      421 tcgccgttcg cgtcgagctc cgaggtggac gtcgtcgcag cggcaggctg cggtgtagcc
      481 tcgaccgggc ttgccacgat gagggcgctc gcgctcgccg cgctcggcat gtgagcgacg
      541 cgcggacggg caaccgacgc gcgcggctcc gaaagcctcg ccttcctgcg ccgctcgagg
      601 aatgcgagct tctgccacag gcgatacgcg accggcagca ccaccgccca caaggcgagg
      661 accatgaccg gcgccagaat cagggtctcg acgccggcgt cgggcgcgcg cgaagcgctg
      721 tagagtgcga tcgcgagcgt cgaaccaact agcgttccga ctccggcgcg caaaccaccg
      781 ctgagctctg ccgtcttctt gaaccgcacg tactcgcgcg cgcagacgct gcaccattca
      841 tcggcgccga cttggtgcgc cggacagacg tgaccgccgc acgccgcgca cacgttgccg
      901 aagctcggct tggcgcagac ggagcatgga aaatgggacg gcacttcgtg cagcgggcga
      961 ccagtcttgg ccggtctctg tgacggcgtc gtcgaagcgc gcgcgcgcag agacggcacc
     1021 tccaccttcg gcacgatgac ttcgagctcc ggctcaccgt gggagcgcgc gcggctgcgg
     1081 tcgcgcagcg ccacgaggtc gttccacatg gccgagagct gcacgtcgga gatctcgagg
     1141 tagttcgccg cttcgctggc ggctccgagc tggattgcct gctccgtgtc cggcagcgcc
     1201 aggacgtagg cgaggagatc ggcgaggcgc agcgtttgaa ccatcgcgga cacgaccgtg
     1261 tcctgcatgg cgcgcgcggg ggcgtgatgc caagcaatca cgcgtggtac cggctcggga
     1321 atgttccagg ccgtgagcac gtgggcgccc aaaacagcgt gatcgaagcc gaacagcctg
     1381 cgctcggcga gatgaatctg gtccggcgcg tgcccgaact cgttcaagag ttctgcgtag
     1441 cggtcgcgct cggcgtccag caacatcagc ttgccgatgt cgtgcaagaa gccgcacgtg
     1501 aagagctcgt cgtgcggcaa cccttggtgc acggcgaggt agcggcagag ggaaccaacg
     1561 atggccgcgt gctcgagcag ctccatcgcg tgggtgctgt tcccatcgaa cagatcgagg
     1621 atggcggcag tcgtagccac ctggttcagg cggcgagtgc cgaccaagac ggccgcgtga
     1681 cgcaccgagg tgcagcgaac ctttaggcca tagccagccg agttgacgag ccgcaggagt
     1741 cgggccgaaa gcgccgggtc gctctccagc acgttgacca cttgctcgat tcgtgtgctc
     1801 gggttgcggg tgagctcatc cagtcgtcgc gccgccaccg ggaacggctt cgcgccgacg
     1861 attcgcgcgc ccacagcggc gaggctcgcc gcggctcgat tctctgcgtc ggcgtcgtcg
     1921 tgatcgccga accacagctc ttcatcgagc gcaaatttct ggtttttggc tgggctgccc
     1981 acagtacgcc ttctctcggc tcactccgcc gcggcggcgg ccttgggggc ttcgccttcc
     2041 gcttcgggct cgcgcacgaa gagctcgcgc gtcgaaaagc gcgacccctc cagaatcacc
     2101 tgagcgccgc atcgagtcgc cgcgttgacg atgatcggag ccagcagatt gacggttgcc
     2161 ggttggccct ggggcgcaca cagcaccacc atgagcgcca aatcttcctc gaccccgccg
     2221 aggttggcat ccgagatcac cggctccatg tcgacgtcgg gatacttggc gccgaaggcg
     2281 tgcgctgaaa cgacgggcaa ggcaagctcc ggagtcttcg cggactgaag ccacgccaag
     2341 aaacccgtcg cattgtgagg caccagcacg aactcacgct cgtgcgggaa gccgatgacg
     2401 cctcgaggaa acctgaggac ttgctcttct tctatttcta gagcgccgaa gcgctggctc
     2461 gtgatgttca tgaggttttt cccggacctt tcgcctcgcg agagcgaggg cattgagtga
     2521 gaccccgacg ttgcagaacc cgtgccacgc cggacgaaac agctcgcgat gagacacacg
     2581 ggggccgctc tcgctgaacg gttggcgaat acgatgcttg agcgcggatc ttcatcgcct
     2641 acgtctcacc accgaaccct cgactcgcgc acgaatcaag tgaaccgctt cagagtggag
     2701 ctgacacacg cgcgactcgg tgacgcccag cacttcgccg atctcgcgca ggctgcactc
     2761 ctcttggtag tggaggctca acacgagacg cagtcgctcc ggcaactctt cgatcgcggc
     2821 caccaccgtg ctctggagct cggcgcgcac ggtagtcgcc tcgggagaag ggtcgagcgt
     2881 cggcggttcg gcgatcgagc cgagctcgag gcgggcaaag ccggcgtcgg agatttcgcc
     2941 gagtaggtcc tggtacgcgc tcagcgacaa gccgagctca cccgcgacct cctcttcggt
     3001 cggcattcgc ccgagtcgct gcgtcaaatc cgaggcggcc gtcgtaatcc gacgcgaagc
     3061 accgcgcagc ttccgagaga gcggatcgag cgcgcgcagg tagtcgagga tcgcgccgcg
     3121 cacacggtag gaggcataag cttcgaagtg ctcgtcctcc atcccctccg cgcgccgctg
     3181 cagagcttcc gccatgccga cccagcccgc cgacaggatg tcgtcgaaag taatgctcga
     3241 cggcagcgtg cggatggttc gcatcgcaat tcggcgcacg agcggcatgt agcgctcgta
     3301 gacctgccga tcttgagtgc ggtcacgaac catgcgggtt gctggcgttc cagacaccga
     3361 tacggtctcc acgtgattca atcgtccgag ggaaagaggg cggccagcac ggagtcacgg
     3421 gaagcatcag tgatgtcttc cggaacgcgc ggtccattgc agagataagc aagcggaagc
     3481 cgtcgcggca tactggcgtg cagcgcgccg ccgatttgct gggtttcgtc gagcttcgtt
     3541 acgatgacgt gggttagggc cggatccgaa tatccttcga ccacccgctc cgcatcgttg
     3601 gctcgaagcc aagccggcac cacgagcgcg acgttgacgc ggacctgcgt gatgccacgc
     3661 agagattcgg cgagcaccga acccacactg tgtgccgagc gcccagccgt atcgaccagc
     3721 acgatgtccg ctcgcgtatc acgcagggct cgacggaagc cggcggcctc gtggacgagg
     3781 tgcaaaggga tccccatcaa cgaggcgtac cgctgccatt gctcggccgc gccgactcgg
     3841 aaagagtcga ggctcaccac ggccacgctc cggccgagat cgagccgtgc gcgggccgcg
     3901 agcttggcga gggtcgtggt ctttccaacg ccggtgggac cgacgcaggc gaggatcatc
     3961 gggccctcga cgttcagcaa atcttcggcg acgtcgaggc gctcccgaac gcgctcgacc
     4021 agccaatttt gcagcgcctc gcggcccttt ttggctggcc gcgccgctcc gttggcgagc
     4081 tcacgcgcga gcgctccttc gataccgcgc gcgtggagca tggcgagggc tcgctcccga
     4141 ggcggcaagt tggcgttgag ctcctcgacc atcgcgcgga ggttcgagag ttcgcgttcg
     4201 acgtcggggt gcgcgccgcc gagcagttga aacgactgcc cgcgcgcgcc ttgcttctga
     4261 ccctgggcgc ctgcgggcgg gcgattcgcg ttcgtacgaa tgcctgcttt ggcggcgaag
     4321 ggccagtcta tcccggctgg tgcggccgcc gtgacctcga cgtacgagcg ttcgagtccg
     4381 ccggcctgcc cattggtgac ctggcgagtg gtctcgatca gcgcattggg ccccaaggtc
     4441 tgcttgacct gcccgagcgc ttccctgaga tccgctcctc gaaacgactg atactgcacg
     4501 ttgtacctcc actacctttg ctagtgaaaa ctgagagaga gtccctacgc tgcggccttt
     4561 ccgaagccga tcgtctcgaa gggacggatg gtggcgtccg gctcgagctc acggaagctc
     4621 aagacggcga gttctggaca gcgacgctcg gcgaaagcgc gcagatagcg acgaacatcg
     4681 gggctcgtga tcacgcacgg cgcgcggccc gcttgcatca tccgcttgac ggcggcctcg
     4741 agggagacgc cgagtcgttg cgcttgctcc gggtcgagcg cgccgccggt tccagccgcg
     4801 atgtcgcgca gcgatcgacg gaacatgtcc tcgaccggtg cgtcgagtac gagcgccgag
     4861 agcgtgccgt cgtgaccggt gaaggcggcg
//
LOCUS       DASTKS010000611         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_766961, whole genome shotgun sequence.
ACCESSION   DASTKS010000611 DASTKS010000000
VERSION     DASTKS010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766961"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..1187
                     /locus_tag="VFQ35_12655"
     CDS             537..1187
                     /locus_tag="VFQ35_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931539.1"
                     /translation="MKQRLLACVGVFGLLTLCGTARAADPPAPRAGFQLALRTGYSLP
                     LGKVWKGFPMSDLTTGQVPLLLDIGGKPIPELFLGAYIGFAFGKAAGVTKDVCEVGGG
                     CATGSFRLGIEGHWHFLPGGWLDPWAGYGIGIESLAVGKSKDGTMDSYAVAGFEFAHF
                     MLGADWRPERTVGLGPFVDVSLGKYRSVTTNSETDDIPNTALHSWITLGVRGVLFP"
     gene            complement(1318..1944)
                     /locus_tag="VFQ35_12660"
     CDS             complement(1318..1944)
                     /locus_tag="VFQ35_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9931540.1"
                     /translation="MHQRVSPSPNAEAFPSKRVFWGWWGGALVALPLLAGAVTAVQSA
                     HGLAKFGGLEYAPRVCALSVVREIAPASGTGAAVIAAVAWAHRVGTNSAASHLRSLYA
                     RFAVVSVGAFPFAAFVATVSSASVLAWHGISVVASWALAANVVAMSDVAAGLLLSALH
                     ALVALGLTRVGLIRLTRSSWGLGRKLSVAWAALIGINTLFGAVGSLFT"
     gene            complement(2057..3046)
                     /locus_tag="VFQ35_12665"
     CDS             complement(2057..3046)
                     /locus_tag="VFQ35_12665"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9931541.1"
                     /translation="MSKLLLLTGALALSGCVSHEWLHGEPTFTPDPPPAPAGLGTDRD
                     TGGNSIVVDGAFSKTSLEIAEGLQNSRRPYVVLDVRHDASWHSVNEAYDGARSAGMAI
                     VRIRREGAAGEISLRPTFGELEGGWLTLEIAADTVKARTWPIEGASGVKEALPSEPSR
                     LRTWLQRSCAAGRCARVVFLDESAASAARGLEVARAVIETWPFSVAPITVLIGRKDGA
                     PALEFEGAQLPPEMIQLVVRASFWRFAGCYKRALAKNPGLAGVVKVRFNILPSGRVAE
                     VGDGSDTKSLETGPLMADDDVKACVFKAFAEFRFPIFEGKPISIVYPIQFAPG"
     gene            3288..4421
                     /locus_tag="VFQ35_12670"
     CDS             3288..4421
                     /locus_tag="VFQ35_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9931542.1"
                     /translation="MVIFLIGEERHVFGSKESYYTVFNDVQGLRRGSPVRMGGVDIGT
                     VSEVAYGDDAKDSRIFVHMSIVSAEARRIGTDSVASIEGKGLLGDKMVVISVGSSSRR
                     LPVGGEVKSKPAEDISQMMGKLSAVSDKAEKVMANLEETTGALADEKLHQDLKAGVNS
                     LSHILASVDRHEGYIGKLVGDPAEAERLSRAIASVERTSDELQAVTRNVNQILARVQN
                     GPGLAHELIYGEDSSKAVAQFGNAAEELTVTLKGIREGNGVARSLLFGGGDQKLVNDL
                     NDMSGDLRQIVADLRAGRGTLGALLVDPSVYEDLKLVLGNVERNKALRALVRYSIRKD
                     EGTPKVSDPDPGTGEQSRPVPAAAGGTVGGKLGTGARSSGVSE"
     gene            4546..>6066
                     /locus_tag="VFQ35_12675"
     CDS             4546..>6066
                     /locus_tag="VFQ35_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HET9931543.1"
                     /translation="MRILERVGRALVVLGVFGVASYFALHFGWGGLWTGLEAAHAVTT
                     PGAQKPPYDLTQLTAVNETLNTIRNKYVDPSRVKPRQMFLSALNQIQKEVAQVIVHHE
                     DKAPTVRVQVEAESADFRVDNVQGPWDVAARLREVFAFLQNNLKDSGIDLREVEYAAC
                     NGMLRTLDPHSVFLSPEAYREMTLSTSGHFGGLGIVISIRDQQLTVMRPMPGTPAGRA
                     GLHRLDRITKINNESTLNMPLDDAVGRLRGKPGSKVTIWVHRDGGEGWQGSRPFELTR
                     EEIQVKSVEARQLSPGIGYVRLKQFQASSSDELDDALTEFSKKERIKGLVLDLRNNPG
                     GLLDQAAKIADRFLEDGVIVTTVGSAEGREEKRAQRKGTEPNYPLVVLTNEQSASASE
                     IVAGALKNQDRAVIVGQTTFGKGSVQLVFPRVTPDNAALKLTIAQYLTPGDVSIQGTG
                     VAPDIELDPMTADTLEMDLFRSERSFRERDLSKSLASPTGPARRSTEQPFVKFRYNL"
BASE COUNT         1162 a         1978 c         1908 g         1018 t
ORIGIN      
        1 tagataaccg atacgaacga agctcgcaag caaacactct cgcatttctt caaaaagatc
       61 attgcgcaat ccgttgctca actgaatcac tccgacccaa acactgagtg cgtcgcgttc
      121 gccaatcctg aaactcgtac cgagcccggc cgcaagtcgg ctcgatgacg gcgcgccatg
      181 taactcaacg gcgcgccatg taactcaacg gcgcgacggt tgcggcgcga cggttgcggc
      241 gcgacggttg cggcgcgacg gttcgtactc gagcggagcg gccgcacgcg tttggtagtg
      301 ccgtccgcgc aagcacagag acgacgctca cttcaaggca tggggcttta gcgctgactt
      361 cgatcatgaa tccggcgctc gctcccgaga tggctccaca gcgcccctgg gattctcgcc
      421 cgatctcgac tctcgctcac gtgactgcgc tgtatcatca cgagttcaat tcgcgctccc
      481 cgcaagtcga cttgctgcct tgacgggccc atggcgcaag cgcaccatcg cgcatcatga
      541 agcagcggtt gctcgcttgc gtcggtgtct tcgggctgtt gacgttgtgc ggcaccgcga
      601 gagccgccga tccgccagcg ccgcgcgccg gttttcagct ggcgcttcgc accgggtact
      661 cactgccttt gggaaaggtg tggaaaggct ttcccatgtc ggacctcacg acggggcaag
      721 tgccgctgct actcgatatc ggcgggaagc ccattcccga gctcttcttg ggcgcctata
      781 tcggattcgc tttcggtaaa gcggcaggtg tcaccaagga cgtatgtgaa gtcggcggcg
      841 gctgcgcgac tggttccttt cggctcggta tcgaagggca ttggcacttt ctacctggcg
      901 gctggctcga tccctgggcg ggctatggaa tcgggatcga atcgctcgcg gtcggaaaga
      961 gcaaggacgg cacgatggac agctacgcgg tggccggctt cgagttcgcc cacttcatgc
     1021 tcggggcgga ctggcgccct gaacgcaccg tcgggttagg cccgttcgtc gacgtttcac
     1081 tcggcaagta caggtcggtg acgacgaact ccgagaccga cgacatcccc aatacggcgc
     1141 tccactcgtg gataacgctg ggcgttcgcg gcgtgctgtt cccctgattc ggcacttcga
     1201 acgtcgataa tctcgagcct ggttcggctg cgctacgaag actgcgccgg ctgcttggat
     1261 ttgaccgaag tcgaactgcc gccgcgccgt tgaagacaat cgccgccgtt cgcgacgcta
     1321 ggtgaatagc gaaccgaccg ccccgaacag cgtgttgata ccaatgagtg ccgcccaagc
     1381 gacgctcagc tttcgtccga gcccccaact cgaacgcgtc aaccgaatca gtccgacccg
     1441 cgtgaggcca agagcaacta gcgcatgcag cgcggacagc agtagtcccg ccgcgacgtc
     1501 gctcatggca acgacattcg cagccagcgc ccaagaagcc acgacggaga tcccatgcca
     1561 tgcgagcacg ctcgcgctcg acaccgttgc aacgaatgcg gcaaagggaa acgcgccaac
     1621 tgacaccacc gcgaaacgag cgtagagact cctaaggtgc gacgccgctg aattcgtgcc
     1681 gacacggtga gcccaagcaa ccgcggcgat gaccgccgcg ccggtcccgc tcgcgggggc
     1741 gatttcgcga acgacgctca aggcgcatac gcgcggcgcg tattcgagcc cgccgaactt
     1801 ggcgagcccg tgggcgcttt ggactgccgt caccgcaccc gccaataagg gcaacgcaac
     1861 cagcgctcct ccccaccagc cccagaacac gcgcttcgat ggaaacgcct cggcgttcgg
     1921 cgaaggactg acacgctgat gcacggcgag cgaatgtacc acctccgcgt gccgtctcga
     1981 ggcttgcacg cgaggtcgga gcaccgcgac gccgtgcccg ccagccacag cgtgcttcac
     2041 gcgcggctcc ggcgcatcaa ccgggcgcga actgaatcgg gtagacgatg ctgatgggct
     2101 tgccctcgaa gatcggaaac cgaaactccg cgaaggcttt gaagacgcac gctttcacgt
     2161 cgtcgtccgc catcagcggt cccgtttcca gagacttcgt gtcgctcccg tctcccacct
     2221 ccgccactcg tccactcggc aggatgttga agcgcacttt gaccacgccg gcgagtcctg
     2281 gattcttcgc gagagctcgc ttgtagcagc ccgcaaagcg ccagaacgaa gcgcgaacca
     2341 ccaactgaat catctccgga ggcagctggg cgccctcgaa ctcgagcgcg ggagctccgt
     2401 ccttccgacc aatgagaacc gtgatgggcg cgaccgagaa tggccacgtc tcgatgactg
     2461 cgcgcgccac ctcgagccca cgcgcagcgg acgccgccga ctcatccaag aagacgactc
     2521 gcgcacaacg accggcagcg cagctacgct gcaaccatgt acgaagcctc gacggctcgc
     2581 tcggaagcgc ctctttgacg ccagatgctc cctcaatcgg ccacgtgcga gccttcaccg
     2641 tgtcggctgc aatttcgagc gtcagccaac cgccttcgag ctcaccgaat gtcggacgca
     2701 gcgaaatctc gcccgccgcg ccttcgcgtc ggatgcgcac aatcgccatg cccgcactcc
     2761 gagcaccatc gtaggcttcg ttcaccgagt gccagctggc gtcgtgtcgg acgtccaaga
     2821 cgacgtaagg tcgtcggctg ttttgcaacc cctcagcgat ctctaaagag gtcttggaga
     2881 acgcgccatc gaccacgatc gagttcccgc ccgtgtctcg gtcggtgccc aagccggccg
     2941 gtgctggcgg cgggtcgggc gtgaaggttg gttccccgtg cagccactcg tggctcacgc
     3001 aaccggacaa ggccagagca ccggtcagca gaagaagctt cgacatcgac gtccaagcac
     3061 gatggttaca aaggctcgcc ccctgggcag cactcctttc gcgcgcggca tggatctacc
     3121 cggcccgatg taggtgcccc accccttccc gggtttgctc gctgcggcct ttcggccggg
     3181 tgacgaagct ctgaggttgg gggagactct gccctcttag ccctatggcc cttggtcgcg
     3241 aattcaaggt cggcgtgttc gttgtcgccg gctctatggt gatagcgatg gtgatcttcc
     3301 tcattgggga ggagcgccac gttttcggta gtaaggaaag ctactacacg gttttcaacg
     3361 acgtgcaggg cctgcggcgg ggctctccgg tgcgcatggg gggcgtcgac atcggaacgg
     3421 tctcggaggt cgcgtacggg gatgacgcca aagattctcg aatcttcgtg cacatgtcga
     3481 tcgtctccgc cgaggcgcgg cgcatcggga ccgatagcgt ggcctcgatc gaggggaagg
     3541 gtttgctcgg cgacaagatg gtcgtcatca gcgtcggcag ctcgagtcgt cggttgcccg
     3601 tcggcggcga ggtgaagtcg aagccagccg aagacatctc ccagatgatg ggcaagctca
     3661 gcgccgtctc ggacaaagcc gagaaggtga tggccaacct ggaagagacg acgggtgcgc
     3721 tggcggacga aaagctgcac caagacttga aggctggcgt gaattcgctg agccacatct
     3781 tggcctcggt cgatcggcac gagggctaca tcggcaagct cgtcggagat cccgccgagg
     3841 ccgagcgcct gtcgcgggcg atcgcgagcg tggagcgcac gagcgatgag ctgcaagccg
     3901 tgacgcgcaa cgtgaaccag attctggcgc gcgttcagaa cggccccggc ctcgcccacg
     3961 agctcatcta cggagaggac agctcgaagg cggtcgcgca gttcgggaac gcggcggaag
     4021 agctgaccgt cacgttgaag ggcatccgcg aaggcaatgg cgtcgcgagg agcttgctct
     4081 tcggcggcgg cgaccagaag ctcgtcaatg acttgaacga tatgagcggc gaccttcgtc
     4141 agatcgtcgc cgacttgaga gcgggacgcg gcacgctcgg agcgctgttg gtggatcctt
     4201 ccgtgtacga ggatctgaag ttggtactgg ggaacgtgga gcggaataaa gcgctacggg
     4261 ctctggttcg ttactcgatt cggaaagatg aaggaacgcc gaaggtcagc gacccggacc
     4321 ccgggacagg agaacaatct cgacccgtcc cagccgccgc cggtggaacc gtcggtggga
     4381 agctcggaac gggcgcacgc tcttccggcg tcagcgagtg acgaaacggc aaacacgccg
     4441 aatcgccgct cggaagcgcc cgtgagcgac gacgccggtt gggcggcacc gcgtgcgaag
     4501 cgtggagatg cgacgcatcc cacccgccac gctatactgc gccccatgcg gatcctggaa
     4561 cgcgtcgggc gcgccctcgt cgtcttgggc gtcttcggcg tggcgtcgta ctttgcgctc
     4621 cactttggtt ggggcggcct atggacgggc ttggaggcgg cgcacgccgt tacgactccg
     4681 ggcgcgcaga agccaccgta cgacctgacg cagctcactg ccgtcaacga gacgctcaac
     4741 accatccgca acaagtacgt cgacccgagc cgcgtgaagc cgcggcagat gttcctgtcc
     4801 gcactgaacc agattcaaaa agaggtcgcc caggtcatcg ttcatcacga agacaaggcg
     4861 ccgacggtgc gcgtgcaggt cgaggcggag tcggccgact ttcgtgtcga caacgtgcaa
     4921 gggccctggg acgtcgccgc gcgcttgcgc gaagtgttcg cgttcctcca aaacaacctg
     4981 aaggacagcg gcatcgactt gcgcgaggtc gagtacgcgg cgtgcaatgg catgctgcgc
     5041 acgctcgatc cgcacagcgt gttcttgagc cccgaagcct atcgcgagat gacgctctcg
     5101 acgtcgggcc acttcggcgg tctcggcatc gtgatttcga ttcgcgacca acagctcacg
     5161 gtgatgcgtc cgatgccggg tacgccggcg ggacgcgctg ggctccaccg tctggatcgc
     5221 atcaccaaga tcaacaacga gtcgacgctc aacatgccgc tcgacgacgc ggtcgggcgt
     5281 cttcgcggca agccgggctc caaggtcacc atttgggtcc accgcgacgg cggcgaaggt
     5341 tggcaaggct cgcggccctt cgagctcacc cgcgaagaga tccaggtgaa gtcggtcgag
     5401 gcgcggcagc tctcgcccgg catcggctac gttcgcttga agcagttcca ggcctcgagc
     5461 tccgacgagc tcgacgacgc gctcaccgag ttcagcaaga aggagcgcat caagggcctc
     5521 gtgctcgatc ttcgcaacaa tccgggcggc ttgctcgatc aggccgccaa aatcgcagat
     5581 cgcttcctcg aagacggcgt catcgtgacg acggtcggca gcgccgaagg ccgcgaagag
     5641 aagcgcgctc agcgcaaagg tacggagccg aactatccgc tcgtcgtgct caccaacgag
     5701 cagtcggcga gcgcgagcga aatcgtcgcc ggcgctctca agaatcaaga tcgcgccgtc
     5761 atcgtcggcc agacgacgtt cggcaagggc tccgtccagc tcgtgttccc gcgcgtcaca
     5821 ccggacaacg cggcgctcaa gctcaccatc gcgcaatacc tcaccccggg ggacgtttcg
     5881 atccagggca ccggcgtcgc gcccgacatc gagctcgacc cgatgacggc cgacacgctg
     5941 gagatggatc tgttccgctc cgagcgctcg ttccgcgaac gcgacctcag caagagcctc
     6001 gcgagcccga ccggaccggc gcgccgcagc accgagcagc cgttcgtcaa gttccgctac
     6061 aacctg
//
LOCUS       DASTKS010000612         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_767280, whole genome shotgun sequence.
ACCESSION   DASTKS010000612 DASTKS010000000
VERSION     DASTKS010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767280"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VFQ35_12680"
     CDS             complement(<1..1576)
                     /locus_tag="VFQ35_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9931544.1"
                     /translation="MTAKTQKSALRRWLSTIVFVLALTTAAVVFRAQLVAWFIGKSIG
                     GASGNEGVKELGGGSPSPSPSASNDAHDGATMSAGSDAIDHYTCSMHPSVKQQGPGKC
                     PICGMDLIPVTKQQQEQGVVMIDEARRQLIGVRTGKVTEAPLSRSFRAVGHVTYDESA
                     LTDVNLKVRGWITKLFVNRTGQHVTVGQPLFQFYSPDLYSAEQDFLLATQGGTAAAST
                     GTQPSRLESLARAGRQRLHLLGLSEAQIDAVEKSGTPSESIAVSSPASGFVIEKNVVE
                     GASVDAGMRLYQIAALNKVWVEAEVYEADLAHVRVGQAANVTLDYLPGHSYEAKISYI
                     YPYLDPTARTGRVRVELQNKELELRPGMYASVELASAAEPRVQVPAAAVVYTGPRRLV
                     FVDLGEGRFKPQEVRVGTEANGMYEVLSGVKPGDTVATSGVFLIAAEARISTAAKYWD
                     KVPEGAEGEIPPSGGASMPSAMPMNAAPMPKRAAPKMPPAAPAMGRAAPVASSAASSS
                     AAVYTWPMHPEVKSPTP"
     gene            complement(1573..2931)
                     /locus_tag="VFQ35_12685"
     CDS             complement(1573..2931)
                     /locus_tag="VFQ35_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9931545.1"
                     /translation="MVQVCRTLLLLSVPALGCASTAGSTSYDAGVVEYSRLERRPATP
                     SAQERDVADGAVLERADYVRAVLRNNPSIESARQGWRAALSRVKQAGSFEDPMVDLGV
                     APLSIGSRAPFGYEVAISQHLPWFGKRSLEAAAAAGEADAAKSDYDAMKRELALTAVT
                     LYDQYVVAEQAIEINHRHVALMQVMRASATAQLASGRGSLQDPLQAEVELTHMEHDRA
                     ILASQRDVTVAQMNELLHRSPELPLPPPPKELPLPPMPDASDTKRLETDAVAHRSEIT
                     AARQRAEAAGARADRAEREYYPDLTLSTSYNSMWDMPQHRWMVGVGFNVPIQTGRRSG
                     SADESRAMRAQFESDAQRLSDSARTQVFVALKQLEESKHVLGLFETRLLPVARDQIDA
                     ARAGFTTSQNPFGAVVEAEKNLRSVELDYQMARAECDRRRAELDRALGRIPGLESKEE
                     EK"
     gene            complement(3061..3474)
                     /locus_tag="VFQ35_12690"
     CDS             complement(3061..3474)
                     /locus_tag="VFQ35_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931546.1"
                     /translation="MNPRSAMRPVAIRLLALISALFVALPSVASTRAHYFCHAMDRVL
                     PACCCEHEDEQESHETGVRAADCCDRLAPASRSGLALRVDAVQHVPSQVLLATLPAFE
                     LPAFRPSTALVPVPRAQAPPREGQRLFLLHCSLLT"
     gene            complement(3568..>3762)
                     /locus_tag="VFQ35_12695"
     CDS             complement(3568..>3762)
                     /locus_tag="VFQ35_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9931547.1"
                     /translation="ADRRVVFRRVADGTCATAVVFPSLGIQKALPLNTDVAVELPPDS
                     HGELAFQCGMGMYRGKVVAE"
BASE COUNT          616 a         1226 c         1247 g          673 t
ORIGIN      
        1 cgggcgtcgg gctcttcacc tccgggtgca tgggccacgt gtagacggcc gcggaggaag
       61 aggccgctga cgaagcgacc ggcgccgctc gtcccatcgc cggggcggcg ggcggcatct
      121 tcggcgccgc gcgcttcggc attggcgcag cgttcatggg catcgcgctc ggcattgacg
      181 cgccgccact cggcggtatt tcgccctcgg cgccttcagg aaccttgtcc caatatttcg
      241 cggccgtgct gatgcgcgcc tcggcggcga tcaggaagac gcccgatgtg gccacggtgt
      301 cgcccggttt gacgcccgag agcacctcgt acatcccgtt cgcttcggtt ccgacgcgca
      361 cctcttgagg tttgaaacgc ccttcaccca ggtcgacgaa cacgaggcgt cgtggtcccg
      421 tgtacacgac agcggcggcc ggcacctgaa cgcgcggttc cgccgccgat gcgagctcga
      481 cactcgcgta cattccgggc cggagctcga gctccttgtt ctggagctcg acacgaacgc
      541 ggccggttcg cgccgtcggg tcgagatacg gatagatgta cgaaatcttg gcttcgtagg
      601 agtggcccgg gaggtaatcg agcgtgacgt tggcggcctg accgacgcga acatgagcaa
      661 ggtctgcttc gtagacctcc gcctcgaccc aaaccttgtt gagtgccgcg atctgataaa
      721 ggcgcatccc cgcgtcgacg gacgcgccct cgacgacgtt cttttcgatc acgaagccgc
      781 tcgcaggcga cgagacggcg atgctctcgg acggcgttcc cgatttttcg accgcatcaa
      841 tctgcgcttc gctcaggccg agcaagtgca agcgttgccg gcctgctcgg gcgagcgatt
      901 cgagccggct cggctgggtg ccggtgcttg ccgccgcggt tccaccttgc gtcgcgagta
      961 ggaaatcctg ctcggcgctg tagaggtcgg ggctgtagaa ttgaaagagc ggctgcccga
     1021 cagtgacgtg ctgtccggtt cggttgacga agagcttggt gatccacccg cgcactttga
     1081 gattcacgtc agtgagcgcc gattcgtcat aggtgacgtg gcccacggcg cgaaacgaac
     1141 gactgagggg cgcttcagtc actttgccgg tgcggacacc gatgagctgg cggcgagcct
     1201 cgtcgatcat cacgacaccc tgctcctgtt gctgcttcgt gaccgggatg agatccatgc
     1261 cgcagatcgg acatttcccg ggcccttgct gcttcactga cgggtgcatc gagcaggtgt
     1321 agtgatcgat agcgtcggaa ccggcgctca tcgtggcgcc gtcgtgcgcg tcgttcgacg
     1381 cggaaggcga cggactcggg cttccacctc ccagctcctt gacgccctcg ttcccgctcg
     1441 ctcctccgat gctcttgccg atgaaccaag ctacgagctg tgcccgaaac acgacggcgg
     1501 ccgtggtcaa ggccagaacg aaaacgatcg tggaaagcca gcgccggagc gcgctcttct
     1561 gcgtcttggc ggtcatttct cttcttcctt cgactcgagc cccgggatcc gaccgagcgc
     1621 gcgatcgagc tcagcccgac ggcgatcgca ctctgcgcgc gccatctggt agtcgagctc
     1681 gacgcttctc aggttcttct cggcttccac tacggcgccg aacgggttct gcgacgtcgt
     1741 gaaccccgcc cgtgccgcat cgatctgatc tcgcgccacg ggcagtaggc gcgtctcgaa
     1801 tagcccaagc acgtgcttcg actcttcgag ctgcttgagc gcgacgaaca cctgggtcct
     1861 cgcggagtcg ctcaagcgct gcgcgtcgct ctcgaattgg gcacgcatgg cgcgcgattc
     1921 gtcggcgctc ccggatcgcc ggcccgtttg gatcggcacg ttgaacccga cgcccaccat
     1981 ccagcggtgc tgcggcatgt cccacatcga gttgtaggac gtggagagcg tgagatccgg
     2041 gtagtattcg cgctcagctc ggtctgctct cgctccggcg gcttccgcgc gctgccgggc
     2101 cgcggtgatc tcgctccggt gggcgacggc gtcggtctcg aggcgcttcg tgtccgacgc
     2161 gtccggcatc ggcggcaaag gcagctcttt cggcggtggt ggcaacggca gctcgggcga
     2221 acgatgcaag agctcgttca tctgagcgac cgtcacgtcg cgctgcgacg cgagaatggc
     2281 gcggtcgtgc tccatgtgcg tgagctcgac ctcggcctgc agcgggtcct ggagcgagcc
     2341 gcgtccgctt gcgagctgcg cggtcgcaga ggctcgcatc acttgcatga gcgccacgtg
     2401 ccggtggttg atttcaatcg cctgctcggc gacgacgtac tggtcgtaaa gagtgacggc
     2461 tgtcagcgcg agctcacgct tcatcgcgtc gtagtcgctc tttgctgcat ctgcctcacc
     2521 cgcggcggcg gccgcttcga gcgagcgctt gccgaaccag ggcaaatgtt ggctgatggc
     2581 gacctcgtat ccgaacggcg cgcgcgatcc gatggagagt ggcgcgaccc cgaggtccac
     2641 catcgggtct tcgaacgagc cggcctgctt cacccgagag agtgcggcac gccagccctg
     2701 cctcgcggac tcgatgctcg ggttgttccg gaggacggcg cgcacgtagt ctgctcgctc
     2761 gagcaccgca ccgtcagcga cgtcgcgctc ctgggcgctc ggagtggcgg ggcgcctttc
     2821 gagacgcgaa tactcgacga ccccggcgtc gtacgacgtg ctgccggccg ttgatgcaca
     2881 gccgagcgcg ggaacggaaa gtagaagtag agttcgacac acttggacca cgaagagctc
     2941 ctcgccgtgc tcgtcacggc atgggtgcgc atcgcggcac agcgctgcgc accgtcttga
     3001 cctcggcgga aacgcacggc gtcggaagca ccgagccgtg gggcacgagt caaggagcgc
     3061 tcaggtcaaa agcgaacagt gcaacaaaaa gagacgctgc ccttctcgtg gcggcgcctg
     3121 cgcgcgtgga accggcacca atgcggtgct cggccgaaac gcagggagct cgaacgcggg
     3181 gagtgttgca agtagaacct gggaaggaac gtgctgaacg gcatcgacgc gtagcgcgag
     3241 acccgagcga ctggccggcg cgagccggtc gcagcagtcg gcggcgcgga caccggtttc
     3301 gtggctctct tgttcgtctt cgtgctcgca gcaacaggcg ggcagcacgc gatccatcgc
     3361 gtggcaaaag tagtgggcac gagtcgacgc gacggacggc agcgccacga acagcgcgga
     3421 gatcaacgcc agaagtcgaa ttgctacggg ccgcatcgcg ctccggggat tcatcctagc
     3481 cctgtggcat aggttgcacc aggcacgacc gccgcctcga cagcttcgcc tgccgggtga
     3541 gcttccgctt ccataagccg tcgtgcttca ttcggcgacg actttgccgc ggtacatgcc
     3601 catcccgcat tgaaatgcga gctcaccgtg ggaatcaggc ggcagctcga cagcgacgtc
     3661 ggtgttcagg ggcagcgcct tctggatccc aaggctcgga aacacgaccg ccgtggcgca
     3721 cgtcccgtct gcgacacgcc ggaagacgac tcgccgatcg gc
//
LOCUS       DASTKS010000613         7939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_990505, whole genome shotgun sequence.
ACCESSION   DASTKS010000613 DASTKS010000000
VERSION     DASTKS010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990505"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..976
                     /locus_tag="VFQ35_12700"
     CDS             128..976
                     /locus_tag="VFQ35_12700"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET9931548.1"
                     /translation="MHPDALILGAPNGPFGLRIEPFGGFLAVGLALGVLLARRLAQRS
                     GFFLGEGLAALGAALLGSLIGARVVHWAFGAESGWRALLALGSGGLSGYGALFGAALG
                     AALALRSSPARAGWFDVGACGALLGISMGRLGCYLAGSDFGRAISGPVPKWLARLSSF
                     PHPRGAEVSEVWFAQTLKGAVNSGSLRTPALHPTALYEAAGALVVLAALLASRTRQRA
                     AGQSALVATVALAVLRFAVDGFRGGVESDRFAASSLDRAAAVLSVLAACAVLAWRKVH
                     RSSSAE"
     gene            complement(1080..1886)
                     /locus_tag="VFQ35_12705"
     CDS             complement(1080..1886)
                     /locus_tag="VFQ35_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9931549.1"
                     /translation="MRFALITDVHFGPRAYHEGKLRKLTDRAGELTARFVERMNRDER
                     PELVVNLGDVIEDKNREHDLEEYGKFTKLLGGLDARVQHVAGNHDQIYLSDEDLRGLW
                     GHTGRLFYSFDFGGVHFVVLCTQEIKDTKVHLPAEQLEFAASDLAATTLPSIVLMHHP
                     ISEQRVEGNRWFERAPHLCRVVERGAARKVLEASGKVVAVFNGHVHWNHLDVIRGIPY
                     VTLQSLTENLDDDAPGRPAAAYAIVDIDERRLVVDVRGEEPARYQLELTR"
     gene            complement(1902..3611)
                     /locus_tag="VFQ35_12710"
     CDS             complement(1902..3611)
                     /locus_tag="VFQ35_12710"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9931550.1"
                     /translation="MTPVAPRVSTAVAEIDHERALELDALATAILKQHFAPPQTLVGL
                     GVPEPKHPKAPSETGFAQVAPDAAPSFARSDEPPETVRAKPQVLNAALIEAALADADD
                     DDETVVMPDRAARSLVLGEAQQHAHQLLDAVDADPIPNPVFSATTPPVEELRQPLQTI
                     TPPPPSEASDLEPSSLPNTRRYDMTPHLPQKHEAPSESARPRGTHSDAPWAVPPSRPR
                     KAPHVGTRLALMVAAFGASLAATFVLINALSPSRKGNLLVTAAGPNQIRVDRAEVLLD
                     GKPVCSQVPCRLEGISSGAHAVTVRAPDYENMAAQPIIVRGGGDSALQLTLTRRDTAV
                     LKVNTKVSGLSLRLDGEERGSAPTTLPRLAPGEHIVRLSGNSAYAPFEQRVVLEADRT
                     LTIEPQLTPLHTVVRVEAGTGADDARVELVNGNERVPLTKLPARVELAPGNVYRVHAT
                     KRAFRDFDEEVRFDANVPERSLTVTLLPRDAKAPPEDRRASSSSTAASVPTTGGSGTL
                     SINSIPISSVLLDGRPVGSTPQKLTLDSGSHSVVFVHPTLGRKERNVSVNPGKTAVAA
                     VRF"
     gene            3610..4098
                     /locus_tag="VFQ35_12715"
     CDS             3610..4098
                     /locus_tag="VFQ35_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931551.1"
                     /translation="MARGGVGVTTGVLLGADPSDDARGDEGRAEELRAIGAHDAAGLA
                     EGAGGGVDAFGADVPASASVGAATTGTKAAAIGAATTGSTAAAIGAATTGSTAAAIGA
                     VTTGSMAAGSAEVGAEAVVAEAVVAGADGANEGRVDDDGAEPVGVESSFGTGAEAGAE
                     FV"
     gene            4533..6146
                     /locus_tag="VFQ35_12720"
     CDS             4533..6146
                     /locus_tag="VFQ35_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931552.1"
                     /translation="MTYAPEIKVRTSRIVANAEHLRRAWKYFGFRDRAPQVDFKARYV
                     VLFVEGGLCSDPGQGRGGRPVLAVAQADDGSILPIFEEPGQVACEASERVRTGFAYAV
                     SLPRGALPPTRVVLGQNSELIGLSSSEVEPARANPESSSTASATLATRNDGTPVWIVR
                     HPDGSASALGADYELMPEWLPGFRVSIGFNLRGRRFDAPFDEWGTHLSGTRSGLDRYS
                     LYVRNGRLEIGDVLGRVPPGKPRVRTESSYSVESSFSSAGAFDGVERRSVREARDARA
                     GALSIVSGQMRLEPGSTAMLCAPDASSTEECAAVREVRTARDCSGYIGGNFAVRSDGE
                     GGFVHVVGLSPDVAYTCRTDEGFRPFWPPGGAWGLRPLTATASFAASSGFAGPEFELG
                     AQASAALRLRHSLPAPGSLRRGLFGDVLELALRARWFDAQGRHGAPAWALGVGPAPLS
                     SNPFGSWATPALLGLVVPEFGVITQHERHRAYLAWRFPIEYRASPLRQRPYTAPERLA
                     WFATPEFVLALGEGVERWHAGAAAGFTVW"
     gene            6200..>7939
                     /locus_tag="VFQ35_12725"
     CDS             6200..>7939
                     /locus_tag="VFQ35_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HET9931553.1"
                     /translation="MAETIAAAATPFDPVPTLREELGLNESSIAAVIRLIAEGATVPF
                     IARYRKEATGGLDEVQIRTIGERFEYLTELDARRTTVLEEIQKQGKLTPELSARIRAI
                     TTKAELEDLYLPYKPKRRTRGTIAIERGLGPLAEKMWAQEGGPAPAESAKPFVNTEKE
                     VPDVAAALAGARDICAERLSEDAELRKALREAYVSSGVIRVKKRSEFEEKATKFDMYA
                     NFEEPLAKVPSHRFLAIRRGENENVLYATLSLDLEPHLPFARARIPVRAGSPWASELE
                     LVVKDALERILSPAVSGDVRVELKLRSDKEAIDVFAQNLRELLLAAPFGAQSVLGIDP
                     GQRTGCKCVVVDATGKLLENENIYLVQGDGALEKARQTLRRLVKTHNVRAIAVGNGTH
                     GRETESFVRELLRSEGLVESTLCVVVNEAGASVYSASDVAREEFPDLDLTVRGAVSIA
                     RRLQDPLAELVKIDPKSIGVGQYQHDVSQPALGRKLDEVVESCVNSVGVELNTASAWL
                     LSRVAGIGLTLAKRIVAHRNERGRFKSRKALLDVSGLGPRTFEQAAGFLRVSGSEHPL
                     DASAVHPERYALVE"
BASE COUNT         1205 a         2508 c         2833 g         1393 t
ORIGIN      
        1 caggcagcgc catcgagcac cagccacacc gcccaggtcg aaaccagaaa agttcgacga
       61 ggcaccactg gtggtagtac tcccgggccc tggtcgttgc cagcgccccg gcgctcttgc
      121 cgttttcatg catcccgatg ctctgattct cggtgcgccg aatgggcctt tcgggcttcg
      181 catcgagcct ttcggaggat tcttggcagt cgggctcgcg ctcggcgtct tactcgctcg
      241 gcgactcgcg caacgaagcg gcttctttct cggcgagggc ctcgccgcgc tgggtgcggc
      301 tctgctcggg agcttgatcg gggcgcgcgt cgttcattgg gcgtttggcg cggagtccgg
      361 ctggcgggca ttgctcgcgc tcggttccgg aggcctgagc gggtacggcg cgctattcgg
      421 cgcggcgctg ggggccgcgc ttgctttgcg gagttcaccg gcgcgcgctg gttggttcga
      481 cgtgggcgcc tgcggcgcgc tgctcggcat ctcgatgggg cgactcgggt gctacttggc
      541 cggctccgat ttcggacgag cgatctcggg accggtgccg aagtggctcg cgcggctgag
      601 ctcgtttccg cacccccgcg gagccgaggt gtcggaggtc tggttcgccc agacgctgaa
      661 aggagccgta aacagcggct cgctccgcac gcccgctttg catcccaccg cgctctacga
      721 agcggctggt gcgctcgtcg tgctcgcggc gctgctcgcg tcgaggacgc gccagcgggc
      781 cgcggggcaa agtgccttgg tcgcgaccgt tgccctcgcc gtgctgcgtt tcgcggtgga
      841 tggctttcgt ggcggggtcg agagcgatcg cttcgcggcc tcgtcgctcg accgagcggc
      901 agcggttctg agcgtgctcg cggcgtgcgc cgtgctggct tggcgcaaag ttcatcgaag
      961 ctcgagcgcc gagtgaagcg aagcggcgtg aggacgcgga cgagccgggc gcggacgctg
     1021 agggccgagt gaagcgaagc gacttgaggt cggggcgagc cgggcgcgga tgagccggat
     1081 tagcgcgtga gttctagctg gtagcgcgcc ggctcttcgc cccgaacgtc gacgaccaag
     1141 cgccgctcgt cgatgtccac gattgcgtac gcggcagcgg ggcggcccgg cgcgtcgtcg
     1201 tcgaggttct cggtcaggct ctggagcgtg acgtagggga tgccgcggat cacgtcgagg
     1261 tggttccagt ggacgtggcc gttgaacacc gcgacgacct ttcccgacgc ttccagcact
     1321 ttgcgcgcgg cgccgcgctc gacgacgcgg cagaggtgcg gcgcgcgctc gaaccaacga
     1381 ttgccttcga cgcgctgctc gctgatgggg tggtgcatga gcacgatgct cggcagcgtc
     1441 gtcgcggcga gatcgctcgc ggcgaactcg agttgctccg cgggcaaatg gactttcgtg
     1501 tccttgatct cttgcgtgca gagcacgacg aagtgcaccc ccccaaaatc aaaggagtaa
     1561 aaaagccttc ccgtatgacc ccaaagcccg cgcagatctt cgtcgctcaa gtagatctga
     1621 tcgtggttgc cggcgacgtg ctggacacgc gcgtcgaggc cgccgagaag cttggtgaac
     1681 ttgccgtact cttcgagatc gtgctcgcgg ttcttgtcct cgatcacgtc gccgaggttc
     1741 acgacgagct cgggacgctc gtctcggttc atccgctcga cgaagcgggc cgtgagttcg
     1801 ccggcccgat ccgtgagctt gcggagcttt ccctcgtggt aggcgcgagg gccgaagtgg
     1861 acgtcggtga tcagggcgaa gcgcatcgcg ccgagcgtag ttcaaaaacg cacggcggcg
     1921 actgcggtct tgcccgggtt cacgctgacg ttgcgctcct tgcgtcccag ggtcgggtga
     1981 acgaacacga ccgagtgact ccccgagtcc aacgtcagct tttgtggcgt cgaaccgacg
     2041 gggcgcccgt cgagcagcac cgaagagatc gggatcgagt tgatgctcaa cgtgccgctg
     2101 ccgcccgtgg tcgggacgga cgcggccgtg ctcgaagaag acgcgcggcg atcctcaggc
     2161 ggcgcctttg cgtcgcgagg caacagcgtg accgtgagcg aacgttcggg gacgtttgcg
     2221 tcgaagcgga cctcttcgtc gaaatcccgg aacgcgcgct tggtcgcgtg cacccgatac
     2281 acgttgcccg gggcgagctc gacgcgcgcg ggcagcttcg tcaagggcac gcgctcgttg
     2341 ccgttcacga gctcgacgcg cgcatcgtcg gcaccggtgc ccgcttcgac ccgcacgacc
     2401 gtgtgcagcg gcgtgagctg cggctcgatg gtgagcgtgc gatccgcctc gagcacgacg
     2461 cgttgctcga agggagcgta cgccgagttt ccgctgagcc tcacgatgtg ctcgccgggc
     2521 gcgagccgcg gcaacgtcgt tggcgccgag ccgcgctctt ctccgtcgag gcggagcgag
     2581 agcccggaaa ccttcgtgtt caccttcaag acggccgtat cgcggcgcgt cagcgtgagc
     2641 tggagcgccg agtcgccacc gccgcgcacg atgatcggct gggcggccat gttctcgtag
     2701 tcgggcgctc gcaccgtcac ggcgtgcgca cccgatgaga tgccctcgag ccggcacggc
     2761 acttgagagc acaccggctt gccgtcgagc agaacctcgg ctcgatcgac gcgaatttga
     2821 ttcggcccgg ccgcggtgac gagcaggttg cctttgcgag aaggcgagag tgcgttgatg
     2881 agtacgaacg tcgccgcgag gctcgcgccg aacgctgcga ccatcagcgc gagacgcgtg
     2941 cccacgtgcg gcgctttgcg gggccgcgaa gggggaacgg cccagggcgc gtcgctgtgc
     3001 gtaccgcggg gtcgagcgct ctcgctcgga gcctcgtgct tctgcggcag gtgcggcgtc
     3061 atgtcgtagc gccgcgtgtt cgggaggctg ctcggctcga gatcggaggc ttcgctcggt
     3121 ggcggcggcg tgatcgtttg aagaggctgc cggagctcct ccaccggcgg cgtcgttgcg
     3181 ctgaaaaccg gattcgggat cgggtcagcg tctaccgcgt cgagcagctg gtgcgcgtgt
     3241 tgctgcgctt cgccgaggac gagagagcgt gccgcgcggt cgggcatgac gaccgtttca
     3301 tcgtcgtcgt ccgcgtctgc gagggcggcc tcgatgagag cggcgttgag cacctgaggc
     3361 ttggcgcgca ccgtttcagg cggttcgtcc gagcgagcga agctcggagc cgcgtcgggg
     3421 gcgacttggg cgaagcccgt ttcgctcggc gctttcgggt gcttgggttc gggtacgccg
     3481 aggccgacca gcgtttgcgg cggagcgaag tgttgcttga gaatcgcggt tgcgagcgcg
     3541 tcgagctcga gtgcccgctc gtgatcgatc tcggcaaccg ccgtcgacac gcgaggcgcg
     3601 acgggagtca tggctcgtgg cggcgtgggc gtgacgaccg gcgtcctgct cggcgcggat
     3661 ccgagcgacg atgcgcgcgg agacgagggg cgcgcagaag agctccgtgc catcggtgcc
     3721 cacgacgctg caggtctcgc cgaaggagcc ggcggcggcg tagatgcttt cggcgcagat
     3781 gtcccggcgt ctgcgagcgt tggcgcggcg acgacaggca cgaaggcagc agcgatcggt
     3841 gcggcgacga caggctcgac ggcagcagcg atcggcgcgg cgacgacagg ctcgacggca
     3901 gcagcgatcg gcgcggtgac gacaggctcg atggcggcag gctctgccga agtcggcgcg
     3961 gaggcggtcg tcgccgaggc ggtcgtcgct ggagctgacg gcgccaacga aggtcgcgta
     4021 gacgacgacg gcgcagagcc ggttggagtt gaatcgtcgt tcggcacagg ggccgaggcc
     4081 ggcgcagaat tcgtttgagg agtggaagcg gtcgcctccc gcgagtcgac tttggacgca
     4141 ctggggcccg agagcggcgg cggctctgga gcgttccaaa tgtccttgta gtcgtcggtc
     4201 acggattgtt cccgcccgcg ctttcgaggt gccatgcgag caacgcggag gatactctcg
     4261 tcctttcggg gtttggcaca agaaaagctc acgccgcgat ccgccaccct cacttccgga
     4321 tccccggacg agctttttgc ggacgttttc gttaggctaa aaacctgttc ggtggacacc
     4381 cgtcgcgctg gattatgtgg ctgaggattc taggcttttc gctgatcgcg tcgtgtctcg
     4441 gcgcttgtgc gatctcagtg ccgacacgct cgccacatcg aatcgtaacg ttgtggtcca
     4501 tcgacccgac acatggcgcg acgacgattt cgatgacgta tgcgcccgag atcaaggtcc
     4561 gaacttcacg catcgttgcg aacgccgagc accttcgccg ggcctggaag tactttggat
     4621 ttcgggatcg cgctccgcag gttgatttca aggcgcgcta cgtcgtgctg ttcgtcgagg
     4681 gagggctctg cagtgatccg gggcaggggc gcggcggccg accggtgctc gctgtagcgc
     4741 aagccgacga tggaagcatc ctccccattt tcgaagaacc cggccaggtt gcgtgcgagg
     4801 caagcgagcg cgtccgtacc ggctttgcat atgctgtgtc gcttccgcgc ggcgcactgc
     4861 cgccgacgcg cgtcgtgctc gggcaaaatt ccgagcttat cggactttcg tcgagcgaag
     4921 tcgagccggc gcgagcgaat ccggagagct cgagcacggc ttccgcgacg ctagccacgc
     4981 ggaacgatgg gacgccagtc tggatcgtgc gtcacccaga tggttccgcg agcgcgctcg
     5041 gagccgacta cgagctcatg ccggagtggt tgccgggctt tcgcgtgagc atcggtttca
     5101 atctgcgcgg gcggcgcttc gacgctccgt tcgacgagtg ggggacacat ctgagcggca
     5161 cgcgttcggg gctcgaccgc tattcgctgt atgttcgcaa tggccggctc gaaatcggcg
     5221 acgtgctcgg cagagtgccg cccggaaagc ctcgtgtccg gacggagtcg agctattcgg
     5281 tggaatcttc gttctcgagc gccggcgctt tcgatggcgt cgagcgacgt agcgtgcggg
     5341 aggcgcgcga cgctcgagcc ggcgcgctca gcatcgtgtc gggccagatg cgcctcgagc
     5401 caggatcgac ggccatgctg tgtgcgccgg acgcatcgag caccgaagaa tgcgcggccg
     5461 tgcgcgaggt gcgaacggcc cgagactgca gcggctatat cggcgggaat tttgcggtgc
     5521 ggagcgacgg tgagggcggc ttcgttcatg tcgttgggct ctcgcccgac gtcgcgtaca
     5581 cgtgcagaac cgacgaggga ttccgtccct tttggccccc gggaggcgct tggggacttc
     5641 gcccgctcac cgcgacggcg tcgttcgcgg catcgtccgg cttcgcgggt ccggagttcg
     5701 agctcggcgc tcaggcttct gccgcgctcc gcttgcggca ttcgcttccc gcgccaggat
     5761 cgctgcggcg cgggctgttc ggggacgtgc tcgaactcgc gttgcgcgcg cgttggttcg
     5821 acgctcaggg tcggcacggt gcgcccgcat gggcgctcgg cgtcggtccc gcgccgctgt
     5881 cgtcgaatcc gttcggatcc tgggcgacac cggccctgct cggattggtg gtgccggagt
     5941 tcggtgtgat cactcagcac gagcgacatc gcgcgtatct cgcgtggcgt tttccgatcg
     6001 agtatcgcgc gtcaccactg cggcagcgcc cgtacaccgc gccggagcgg ctcgcgtggt
     6061 tcgcgactcc ggagttcgtg ctcgcgctcg gtgagggcgt agagcgttgg cacgccggcg
     6121 ccgctgctgg tttcaccgtt tggtagggtt ctcggcacgt cgcgcttgga catccgttca
     6181 ggcgttggta tggagcccca tggccgaaac gatcgcagcc gccgccactc ctttcgatcc
     6241 agtcccgacg cttcgtgaag agctcggact caacgagagc tcgatcgcag cggtcattcg
     6301 actcatcgcg gagggcgcga cggttccgtt catcgctcgc tatcgcaagg aggcgacggg
     6361 cgggctcgac gaggtacaga ttcgcacgat cggcgagcgc ttcgagtacc tcaccgagct
     6421 cgacgcgcgg cgcaccaccg tgctcgaaga gattcagaag caaggcaagc tcactccgga
     6481 gctgtcggcg cgaatccgcg caatcacgac gaaggccgag ctcgaagatc tgtaccttcc
     6541 ttacaagccg aagcggcgca cgcgcgggac gatcgcgatc gagcgcggtt tgggtccgct
     6601 cgcggagaaa atgtgggcgc aagaaggggg cccggctccg gccgagagcg cgaagccatt
     6661 cgtaaacacc gagaaggagg tgcccgacgt ggcggcggcg ctcgcgggcg cgcgagacat
     6721 ctgcgccgaa cgcctttcgg aggacgcgga gctccgaaaa gcgttgcgcg aagcctacgt
     6781 ttcgagcggt gtcatccgcg tcaagaagcg cagcgagttc gaggagaagg ccacgaagtt
     6841 cgacatgtac gcgaacttcg aagagccgct cgcgaaggtg ccttcgcatc gcttcctcgc
     6901 gattcggcgc ggcgaaaacg agaacgtgct ctacgcgacg ctgtcgctcg acctcgagcc
     6961 gcacctgccg tttgcgcgcg cacgcatccc cgttcgcgct ggctcgccgt gggcttccga
     7021 gctcgagctc gtcgtcaaag acgccttgga gcgcatcctt tcgccggccg tctccgggga
     7081 cgttcgcgtc gagctcaagc tgcgctccga caaagaggcg atcgacgtct tcgcgcagaa
     7141 cctgagggag ctgctactgg cggcgccttt cggcgcgcag tcggtgctcg gcatcgatcc
     7201 cggtcagcgc accggctgca agtgcgtcgt cgtcgacgcc accggcaagc tcctcgaaaa
     7261 cgagaacatt tacctggtac aaggcgacgg cgcgctcgaa aaggctcgtc aaaccctgag
     7321 gcggctcgta aagacgcaca acgtgcgcgc gatcgccgtc ggcaacggca ctcacggacg
     7381 cgaaaccgag tcgttcgtgc gcgagctgct gcgcagcgag ggcctcgtcg agtccactct
     7441 gtgcgtcgtc gtgaacgaag cgggcgcgag cgtgtactcc gcgagcgacg tcgcgcgcga
     7501 agagttcccc gatctcgatt tgacggtgcg tggcgcggtg agcatcgccc ggcgcctgca
     7561 agatccgctc gccgagctcg tgaagatcga tccgaagagc atcggggtcg gccagtacca
     7621 gcacgacgtc tcgcagccgg ctctcggtcg caaactcgac gaggtcgtcg agagctgcgt
     7681 gaacagcgtc ggcgtcgagc tcaacaccgc gagcgcgtgg ctgctctcgc gcgtcgcggg
     7741 cataggcctg acgctcgcca agcgcatcgt cgctcaccgg aacgagcgtg ggcgtttcaa
     7801 gtcgcgcaag gcgctgctcg acgtgagcgg cctcggcccg cgcaccttcg aacaggccgc
     7861 cggcttcctg cgcgtcagcg gcagcgagca cccgctcgac gcgagcgcgg tgcaccccga
     7921 gcgctacgcc ctggtggag
//
LOCUS       DASTKS010000614         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243 k141_99474,
            whole genome shotgun sequence.
ACCESSION   DASTKS010000614 DASTKS010000000
VERSION     DASTKS010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99474"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /gene="chrA"
                     /locus_tag="VFQ35_12730"
     CDS             <1..1257
                     /gene="chrA"
                     /locus_tag="VFQ35_12730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HET9931554.1"
                     /translation="ELVDKRRWLSDERFLHALNYCMLLPGPEAQQLATYIGWLLHRVP
                     GGIIAGTLFVLPGFLGVLVLSLLYAFFQQQPTIAALFFGLKAAVLAVVVEAVLRIGKR
                     ALKSRFLVAIAASAFIALFLFDVPFPAVVLAAGLLGFLGGRAVEANAGTASRGVTNAA
                     PKSLIEWMAHRGELAHTAASPASALRVLAVCGALWVVPLLALARIFGTRSVFVQEGLL
                     FSKAALVTFGGAYAALAYVAQRAVEVYGWLTPREMLDGLGLAETTPGPLILVVEFVAF
                     VGAFRNPAGLSPWVSGLVGATVTVWVTFVPCFLWIFLGAPYIESLRGNRSLSAALTGI
                     TAAVVGVILNLAFVFGTNVVFHRVRHVHCGPLNVLSPEWGSVDVAAALLAAFALLLLL
                     RFKLGLPRTLLACALLGAGYKLALGR"
     gene            complement(1371..3203)
                     /locus_tag="VFQ35_12735"
     CDS             complement(1371..3203)
                     /locus_tag="VFQ35_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9931555.1"
                     /translation="MQNRRFLRLASIPAFAFALHAHLAHADTIYVSPTGSGDGTSAAS
                     PTTIPTASMNAKAGDTVILAGGVYHDRIMPANKGTSSAWITFKAADGALPIIENTADG
                     TGAGSDDTEYVIFDGIATRHNLKLGFSNKYIDYCGGTSQAGGSQTSNGHFKYLNVIAE
                     GSGVSGIGTFCATDVTIQNAIVAHNGLGESWTSGVNLYGTYGGPSVNTIKNVVSFENI
                     DISKDKSDGSGFIMDVGTKGALFQNNIAFRNGGSCFRITNSNNAQMINNTCWDNAQFD
                     TQYADEIFFSTNGDSSGAIIRNNIIVPHSGKAAVGGTNSGSVAKDSCLILAAGAATPF
                     FTSTANGDFTLTSGATDLINKGATSGAPTTDIGFDPKCIKPGTSSPNDYPICTGTEGV
                     KNSCASKATWFKYKIDYDYIASIGGIKACFQPGMRMGNPDIGAYEQGTVIAHGGASST
                     GGTSSTGGGNNGGAAGAAGSGGKANTGGGNNGGASSGGTSSSSGGSSSGGSSSGGATA
                     NGGGATANGGSSSSNGGSSNASGGAVATSGGASNASGGATGMGGSMAKGGSATAAGSG
                     GGSTDSGCSCTVVGNERASTGAYSLFGVAMALTGLRMRRRRARG"
     gene            complement(3526..>4015)
                     /locus_tag="VFQ35_12740"
     CDS             complement(3526..>4015)
                     /locus_tag="VFQ35_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET9931556.1"
                     /translation="CDDVASLGLRTVIDLRTSDERVSVPEASCVADTTRIVEAPLPIP
                     YNVSPADYIADLDASASVAAAFAALGDDTAYPVYFHCTWGRDRTGILAALVLAALGAT
                     RDEILAEYSLSEWTVGAYPASLRAALDEVERRGGVASYLSSVGVTEGQLTVLRTRLVT
                     NP"
BASE COUNT          593 a         1261 c         1336 g          825 t
ORIGIN      
        1 gagctagtgg acaaacggcg atggctgtca gacgagcggt tcctccatgc tctaaattac
       61 tgcatgttgc ttccggggcc ggaggctcag cagcttgcca cgtacatcgg ttggctcctt
      121 caccgagtgc cggggggaat catcgctggc actctcttcg tcctccccgg gtttttgggc
      181 gtgctcgtgc tgagcctgct ctacgccttc ttccagcagc agcctacgat cgcggcgctg
      241 ttcttcggac tcaaggcggc ggtgctcgcg gtggtggtcg aagccgtgct ccgaatcgga
      301 aaaagagcgc tcaagagcag gttcttggtc gcgatcgcgg cttccgcgtt catcgctctt
      361 tttctattcg acgtgccatt tccggcggtc gtgttggcgg cggggctact cgggttcctc
      421 ggggggcgcg ccgttgaggc caacgctgga acggcaagtc gcggcgtcac gaatgcggcg
      481 cctaaatcgc tcatcgagtg gatggcgcat cggggcgagc tcgctcacac cgctgcctcg
      541 cccgcgagcg cgctgcgtgt cttggccgtc tgcggagcgc tttgggtcgt cccgttgctc
      601 gccttggcgc gcattttcgg gacgcgctcg gtgttcgtcc aagagggact gctcttttcg
      661 aaagcggcgc tcgtgacctt cggcggggcg tatgcggcgc ttgcttacgt cgcgcaacgc
      721 gcggtcgagg tctacggttg gctcacgccg cgcgaaatgc tcgatggttt ggggctcgcc
      781 gagacgactc cggggccgtt gattttagta gtcgaattcg tggcgttcgt cggagcgttc
      841 agaaaccctg cggggttgtc gccgtgggtt tccggcttgg tcggcgcgac agtgacggtt
      901 tgggtgacgt tcgtgccgtg cttcttgtgg atcttcctgg gggcgccgta catcgaatcg
      961 ctgcgcggca atcgatcgct ctccgctgcg ctcacgggca tcacggccgc ggtcgtcggc
     1021 gtgatcttga acctggcgtt cgtgttcgga acgaacgtgg tctttcaccg cgtgcgtcac
     1081 gtgcattgcg ggccactcaa cgtcctcagt cccgagtggg gcagcgtcga cgtggccgcg
     1141 gccctgctcg cggcgttcgc cctgctgctg ctattgcgct tcaagctcgg cttaccgcgg
     1201 acgctcctcg cgtgcgcatt gctcggtgcc ggctacaaac tcgcgctcgg tcgctgagcg
     1261 cacaccgtga ttttcacgcg ctgcccgcgg cgtggctagt ttcgttgctg actcgagcga
     1321 ggcgggaacg acttcgagtt cgtgcccgcc ttgcttcgag ctcgctcgac tcagccgcgc
     1381 gcgcgacgac gacgcatgcg gaggccggtg agcgccatcg cgacaccgaa gagcgagtag
     1441 gcgcccgtgg aggcgcgctc gttgccgacg acggtgcagc tgcagccgga gtcggtgcta
     1501 ccgccaccgc taccagcggc cgttgcgctg ccacccttgg ccatggatcc gcccatcccg
     1561 gtggcgccgc cgctcgcgtt ggatgcgcca ccggacgtcg ctacggctcc gccgctcgca
     1621 ttcgagctac cgccgttcga gctcgagcta ccgccgttcg ccgtcgcgcc accgccgttc
     1681 gccgtcgcgc caccgctgct ggagcctccg ctgctggacc cgccgctgga gctcgaggta
     1741 ccgccgctcg acgcgccgcc attgtttcct ccgcccgtgt ttgctttgcc gcccgagccc
     1801 gcagcgccgg ccgctccgcc gttattgccg cccccggtgc ttgaggtacc gccggtgctc
     1861 gacgcgccgc cgtgtgcgat gacggtgccc tgctcgtacg cgccgatatc cgggttgccc
     1921 atgcgcatgc cgggttggaa gcaagccttg ataccgccga tcgacgcgat gtagtcgtag
     1981 tcgatcttgt atttgaacca ggttgccttg ctggcacacg agttcttcac gccttcggtg
     2041 ccggtgcaga tgggatagtc gttcgggctg ctcgtcccgg gcttgatgca cttcgggtcg
     2101 aacccgatgt cggtagtggg cgcgccgctc gtcgctccct tgttgatgag atccgtcgcg
     2161 ccggaggtca gcgtaaagtc gccgttcgcg gtgctggtga agaacggcgt ggccgcgcct
     2221 gccgccagga tgaggcagct gtccttggcg acggagcccg aattcgtccc tccgaccgcg
     2281 gccttaccgc tgtgggggac gatgatgttg ttgcgaatga tcgcgccaga gctgtcgccg
     2341 ttggtcgaaa agaagatctc gtcggcgtac tgggtgtcga actgagcgtt gtcccagcac
     2401 gtattgttga tcatctgggc gttgttgctg ttcgtgatgc ggaaacacga gccgccgttc
     2461 ctgaacgcga tgttgttctg aaacagcgcg cccttcgtgc ccacgtccat gatgaagccg
     2521 ctgccgtccg acttgtcctt cgagatgtcg atgttctcga acgagacgac gtttttgatc
     2581 gtgttgacgc tggggccgcc gtacgtgccg tagaggttca cgccgctggt ccatgactcg
     2641 ccgaggccgt tgtgcgcgac gatcgcgttt tgaatcgtga cgtcggtcgc gcagaacgtg
     2701 ccgataccgc tcacgccgct gccctcggcg atcacgttga ggtacttgaa gtggccgttg
     2761 ctcgtctgcg agccgccggc ttggctcgtg ccgccgcagt agtcgatgta cttgttcgag
     2821 aagccgagct tcaagttgtg gcgcgtcgcg atgccgtcga agatgacgta ttcggtgtca
     2881 tcgctgcccg cgcccgttcc atccgccgtg ttctcgatga tcggcaacgc gccgtccgcc
     2941 gccttgaagg tgatccatgc gctcgaggtg cccttgttcg cgggcatgat gcgatcgtga
     3001 tagacaccac ccgccaggat tacggtgtcg ccggccttgg cattcatgct cgcagtcgga
     3061 atcgtcgtgg gggacgcggc cgaggtgccg tccccactgc cggtgggcga gacgtagatc
     3121 gtgtctgcgt gcgcgaggtg cgcgtgcaga gcgaaggcaa aggcagggat cgaagctagg
     3181 cgcagaaaac gacggttttg catgagaggg cccaaaactt acgagtctgt tcgcgcggag
     3241 caagcgcttc agacgggcga tctggaaaaa accatagaag tcgtcttcga cccaagaacg
     3301 gcataaaacg cgccaaatgg ggtcatcctt gactccgcgg gaggggctcc tcacctcggg
     3361 aaatatttcc ttaattaacg aattcagcgg cagcgttcga gccgtcgggc cgccgcctcg
     3421 gttttgcggc attcgtgacg gatttctcgt agcgagtgca cagtgctcgg cgcggctcgg
     3481 ggcgtagcaa aaccgccgcc gcgcgggagg tacgcggacg tcggcctacg ggttagtaac
     3541 caagcgcgtt cgcaggacgg tgagctgccc ttcggtgaca ccaaccgagc tcaggtagct
     3601 cgcgacgcct cctcgccgct cgacctcgtc gagcgcggca cgcaggctcg ccggatacgc
     3661 gccgacggtc cactccgaca aagagtactc agcgaggatc tcgtcgcgag tcgctccgag
     3721 cgcagcgagc acgagcgcgg ccaagatccc cgtgcgatcg cggccccaag tgcaatggaa
     3781 gtacaccgga taggcggtgt cgtctcccaa ggcggcgaat gcggccgcca ccgaagcgct
     3841 cgcgtcgaga tcggcgatgt agtcggcggg gctcacgttg tacgggatgg gcaagggcgc
     3901 ttcgacgatg cgagtcgtgt ccgcaacaca gctcgcctcg ggaacgctca cgcgttcgtc
     3961 gctcgtgcga agatcgatca cggtgcgaag cccgagtgaa gccacgtcgt cgcag
//
LOCUS       DASTKS010000615         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_990719, whole genome shotgun sequence.
ACCESSION   DASTKS010000615 DASTKS010000000
VERSION     DASTKS010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990719"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VFQ35_12745"
     CDS             <1..1348
                     /locus_tag="VFQ35_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9931557.1"
                     /translation="DEVASSSTPCFPPGPRTPALLQAIEWGFRRVQFLEECAARYGEI
                     FTIRLGKMALVVTARPSDLRQLFSARAETFVTGAANQSLASLIGDESLFVLDGADHRR
                     VRRLCLPAFSARRMDEYGDALLGDALKSLDDMPHGSFRLCDWLDTVLQRVIARVVFGL
                     TPADADYALLLSALRRIASTLTSSKAGLMAFPELQRFLGPLAPLSDFRSANEALEALA
                     RRLVHEARGSNRRPDSFLASLVAERDGDRALTDDEVLSQVRTFVVAGVETVVSGCAWT
                     LRWVLDSPELSSALRSELDACSQGGPLVAAQVAGLGLLDACVRESLRLSPPTASLVRI
                     PNEDLDLEPFLIPAGTPVLGSIYLAHHRSQAFAAPLTFDPLRFRGTAVSPYEWLPFGG
                     GERRCIGMHVAMHEIKLIVAAVVHHRNLTLHQPRRIRPVLRGTTLAPSGGLRVRSA"
     gene            complement(1380..2753)
                     /locus_tag="VFQ35_12750"
     CDS             complement(1380..2753)
                     /locus_tag="VFQ35_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9931558.1"
                     /translation="MKSKITIMGHAGLYIEAGAERILVDPLLRTSALASGTVVHSYTR
                     ELNLEAMPSPTLGVITHGHLDHFDPESLRRLDRRLPLVVPNDPAMQHALRELGFQQLI
                     TLNPWETHRADGVALTATPSHAEIDEWGVLFEASEARFLHHADAEPSLEDAQRVRRER
                     GEIDVTSVKFQPASPGHGVLRALGPYFDKRHVLEWLECAAELGAELSFPYASGVQFSG
                     RHAWMNDYAFPFQSNEIAALLSPRLASPPGAESTPTRRVECVLPGDVIEFAPRHAQRF
                     PQASRFVRHVEDGHGEPVWRPFDQATLEGLSSDAEREELEERLEAFLRDEIAPWIAMR
                     RDEEGSPIQSYVKYGVVYQMSVHVGAGQQLNYAIDFRNKLSLTAEWHPEANYFAHLSG
                     RSLLEVLRGNAGPELFYMSGDVRLYEKILWVQNGKFGMPNVRGWDLFERLPEPVTHYL
                     RHTRDAH"
     gene            complement(2781..4031)
                     /locus_tag="VFQ35_12755"
     CDS             complement(2781..4031)
                     /locus_tag="VFQ35_12755"
                     /inference="COORDINATES: protein motif:HMM:NF023417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0009820 - alkaloid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan dimethylallyltransferase family
                     protein"
                     /protein_id="HET9931559.1"
                     /translation="MDAARESWHRFDQSRALAADTGGPLRQGKRGGVRMRDIERTLAN
                     IAKTQLAALSEATGLGPRRRELESWFAILSESWGHASPEELPLWSGIGDDCSPLEWSV
                     VLRRDERAEMRILAEAHEEPASPASYWHAAEALTQRISANVPLDLERLERVRDLFAPR
                     GSEDEVFFSAFHAAVFWADRAPMLKLYLNPAARGFASATEVTREALARLGFHRAWASL
                     EPLLEPPHVTPAFMSLDLGAGEGGRVKVYLRHRHVPIDSVERAAATAPNYARADLARF
                     FETMAGERASAGVTRAVLTTHYFTDEESQAPRAVALQMPARPYTDDDAVFTHRFRALL
                     EREGIPERVYEQALRALGVGEDRQGAATQSWAAFSRIGGEPRIAAYFSPQFYARTFGP
                     LSLDPVRAWPRPSGHDVGAWGLAI"
     gene            4289..>4985
                     /locus_tag="VFQ35_12760"
     CDS             4289..>4985
                     /locus_tag="VFQ35_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931560.1"
                     /translation="MNISLLFPPGTDPRAPHLGLPSLAAVLRREGIRTTMHDLDLEGL
                     LALVAPERLQRAAKALRDAQSGPPSDRVRRLLGISEFLVDSSDRAVAVLRDPVDFYDP
                     LKHQQARTVLSDALALVSAASGNVQYNILPIAYDVEGADAASLRDLLRVTSDRATNLF
                     QEDWEEHLFPRLSAEAPDIVGISITNRQQIVPGLHLARALRERNHFVVIGGTVFTKFV
                     DVLRRRPAFFEHFA"
BASE COUNT          819 a         1624 c         1593 g          949 t
ORIGIN      
        1 ggacgaagtc gcctcgtcgt ctacgccgtg ttttccgccc gggccgcgta ctcccgcgtt
       61 gcttcaggcg atcgagtggg gatttcggcg tgtgcagttt ttggaagagt gcgccgcgcg
      121 ctacggcgag atcttcacga tacgcctcgg caaaatggcg ctcgtcgtca cggcgcggcc
      181 gagcgatctg cggcagctgt tttcggctcg ggccgaaacg ttcgtgaccg gggccgccaa
      241 tcagagcctg gcgagcctca tcggtgacga gtcgctgttc gtgctcgatg gcgccgacca
      301 ccgccgggtg cggcggcttt gcttaccggc tttcagcgca cgccgcatgg atgaatacgg
      361 cgatgctttg ctcggcgacg cgctcaaatc cctcgacgac atgccgcacg gttccttccg
      421 gctgtgtgat tggctggaca cggtcttgca gcgtgtcatc gcgcgtgtcg tgttcggctt
      481 gactccggcg gacgctgact acgcgcttct actcagcgcg ctccgtcgca tcgcgagcac
      541 gctgacgagc agcaaggcag ggctcatggc attccccgag ctgcagcggt ttttgggccc
      601 cctcgctccc ctcagcgact ttcgctccgc gaacgaggca ttggaagcgc tcgcgcgccg
      661 tttggtgcac gaggcgcgtg ggagcaatcg gcgacccgat agctttctgg cttcactggt
      721 ggccgaaagg gacggggaca gagcgctcac cgacgacgag gtcctctcgc aggtccggac
      781 cttcgtcgtg gctggagtcg aaacggtcgt atccggttgc gcgtggacgt tacgctgggt
      841 gctggactcg ccggagctct cgagcgcgct ccgaagcgaa ctcgatgcgt gcagtcaggg
      901 aggtccactc gttgccgcgc aagtcgcagg gctcgggctg ctcgacgctt gcgttcgcga
      961 aagcctccga ttgtccccgc ctacggcgag cctcgttcgc atcccgaacg aagacctgga
     1021 cctggagcct tttctgatcc ccgcgggcac gcccgtgctc ggctcgatct acttggcaca
     1081 ccaccgcagt caagcgttcg ctgcgccgct gaccttcgac ccgcttcggt ttcggggcac
     1141 ggcggtgagc ccctacgagt ggcttccatt tggcggtggt gagcgtcgct gcatcgggat
     1201 gcacgtcgcc atgcacgaaa tcaagctcat cgtcgccgcc gtcgttcatc atcgaaacct
     1261 cacgctgcat cagccgaggc ggattcgtcc ggtgcttcgc ggaaccacgt tggcaccgtc
     1321 gggagggctt cgggtccgca gcgcgtgaag gacggtgctc tcccggactc gggtgttcct
     1381 cagtgtgcgt cgcgggtatg gcggaggtag tgagtaacgg gctcggggag acgctcgaac
     1441 aagtcccagc cgcgcacgtt cggcattccg aacttcccat tttgaaccca caggatcttc
     1501 tcgtaaagcc gaacgtcgcc agacatgtaa aagagctcgg gtcccgcgtt gccgcgcagt
     1561 acttcgagca ggctgcgccc ggacaggtgc gcgaagtagt ttgcctccgg gtgccactcc
     1621 gccgtcaatg acagcttgtt ccgaaagtcg attgcgtaat tgagttgttg gcccgcgccg
     1681 acgtgcaccg acatttgata tacgacgccg tacttgacgt agctttggat cgggctccct
     1741 tcctcgtcgc ggcgcatcgc gatccacgga gcgatctcgt ctcgcaagaa ggcttcgagc
     1801 ctctcctcga gctcctcgcg ctcggcgtcg gacgagaggc cttcgagcgt cgcttggtcg
     1861 aacgggcgcc ataccggttc gccgtgcccg tcttccacat gacgcacgaa gcgcgacgct
     1921 tgcggaaagc gctgagcgtg ccgcggcgcg aactcgatga cgtcgccggg gagcacgcat
     1981 tcgaccctcc gcgttggcgt ggactccgcg ccgggcggcg aggccagccg cggagagagc
     2041 agcgcggcaa tctcgttgct ttgaaacggg aacgcgtagt cgttcatcca tgcgtggcga
     2101 ccgctgaact gcacgccgct cgcgtagggg aacgagagct cggcgccgag ctccgccgcg
     2161 cactcgagcc actcgagcac gtgtcgcttg tcgaagtatg ggcccagggc ccgcagcacc
     2221 ccgtggcccg gggacgccgg ctgaaacttc actgacgtca cgtcgatttc ccctcgctca
     2281 cggcggactc gctgcgcgtc ctcgagcgaa ggctccgcgt cggcgtggtg gaggaatcgc
     2341 gcttcggaag cttcgaacag cactccccac tcgtctatct ctgcgtgtga aggggtcgct
     2401 gtgagcgcga cgccatcggc gcggtgtgtc tcccatgggt tcaacgtgat gagctgctga
     2461 aagccgagct cacgtaaggc gtgctgcatc gccggatcgt tcgggacgac gaggggcaaa
     2521 cgccggtcca gccttcgcag cgactccgga tcgaagtggt cgaggtgccc gtgggtgatg
     2581 acgccgagag tgggcgacgg catcgcctcg aggttcagct ctcgagtgta gctgtgcacg
     2641 acggtgccgc tcgcgagcgc gctcgtgcgc aacagcgggt cgaccagaat gcgctccgcc
     2701 cctgcctcga tgtagagacc tgcatgcccc atgatggtga tcttggattt cacttttcgt
     2761 cttctccgtt cgacttttcg ctagatggca agtccccacg ctcctacgtc gtgcccactc
     2821 ggacgcggcc acgcccgcac cggatcgagc gacaaggggc cgaaggtgcg agcgtaaaac
     2881 tgcggcgaga agtacgcggc aatgcgcggc tccccgccga tcctcgagaa agctgcccag
     2941 ctctgcgtgg ccgcgccctg ccgatcttca cccacgccga gcgcgcgcag cgcttgctcg
     3001 tacactcgtt cggggatccc ctcgcgctcg agcaacgcgc gaaaccgatg ggtgaagacg
     3061 gcgtcgtcgt cggtgtacgg acgggcaggc atttgcagag ctaccgcgcg aggtgcctgc
     3121 gactcctcgt cggtgaagta gtgggtagtg agcacggccc gggttacccc ggcgctcgcg
     3181 cgctcgccgg ccatagtttc gaaaaagcga gccagatcag ctcgcgcgta gttcggcgca
     3241 gtggcggcgg ctcgctcgac cgagtcgatt ggcacgtgcc gatggcgaag gtaaaccttc
     3301 acccggcctc cttctcccgc tccgaggtcc agcgacatga atgcaggagt gacgtgcggc
     3361 ggctccagca gcggctcgag tgatgcccat gcgcggtgaa aaccgagccg agccagcgcc
     3421 tcgcgggtga cctccgtcgc gctcgcaaac cctcgcgccg cggggttcaa ataaagcttc
     3481 agcatgggtg cgcggtcggc ccaaaacacc gccgcgtgaa aggcagaaaa gaagacctcg
     3541 tcttccgacc cgcgaggcgc gaaaagatct cgaactctct ccaatcgctc caaatcgagc
     3601 ggcacgttcg ccgagatgcg ctgcgtgagc gcttcggccg cgtgccagta gctagcggga
     3661 gacgcgggct cctcgtgtgc ctcagccagt atgcgcattt cggcgcgctc gtcgcggcgc
     3721 aatacgacgg accactcgag cggagaacag tcgtccccga tccctgacca gagcgggagc
     3781 tcttccggtg aagcgtgtcc ccacgactcg gacaggattg cgaaccagct ctcgagttca
     3841 cgcctgcggg gaccgagccc ggttgcctca ctcaatgctg cgagttgcgt ctttgcgatg
     3901 ttcgccaacg tgcgttcgat gtctctcatt cgtacgcctc cgcgtttgcc ttgccgcaac
     3961 ggaccgccgg tgtcggccgc gagcgcgcgc gactgatcga aacgatgcca gctttctcgc
     4021 gcggcatcca tgggtgattc gttagcgctc cttcgtcgac tcgaagtgtg agcatcgtct
     4081 cgtcatgagc gattcgttga cgaagaactt cacacggcgt ggcgcgctgg cgctgacaac
     4141 gggtgttcgt tctctcgttc actcacggat cagaaacaaa ttaccgaaga cgcgcgcttc
     4201 gaccgtctga tagaaagtga cgtcgaacgt agaagtagcc gagtacggcc cgctcgccgt
     4261 tttcagctca cctcgctggg cgattacaat gaatatcagc ctcctctttc cgccaggcac
     4321 tgatccgcgc gctccacatt tggggctgcc ttcgctcgcg gcggtgctgc gccgcgaagg
     4381 gattcgaacg acgatgcacg acctcgatct cgagggcctg ctcgcgctgg ttgcgcccga
     4441 acgccttcaa cgggccgcca aggccctacg cgatgcgcag tcggggccgc cttcggatcg
     4501 agtccggcgc ctgctcggta tcagtgagtt cttggtcgat tcgagcgacc gcgcggtcgc
     4561 ggtgctgcgt gatccggtcg acttctacga cccgctcaag caccagcagg cgcgcaccgt
     4621 cttgtccgac gcgctcgcgc tcgtctccgc ggcgagcggg aacgtgcagt acaacatttt
     4681 gcccattgcc tacgacgtcg aaggtgccga cgccgcgtcg ctacgcgatt tgctccgcgt
     4741 tacctccgac cgcgccacca atctgttcca ggaggactgg gaagagcacc tatttcctcg
     4801 attgagcgcg gaggcgccgg acatcgtcgg tatcagcatc accaaccggc agcaaatcgt
     4861 gccgggctta caccttgcgc gcgcccttcg agagcgaaat cacttcgtcg tgatcggcgg
     4921 caccgtcttc acgaagttcg tcgacgttct ccggcggagg ccggcattct tcgagcattt
     4981 cgcgg
//
LOCUS       DASTKS010000616         4662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1660818, whole genome shotgun sequence.
ACCESSION   DASTKS010000616 DASTKS010000000
VERSION     DASTKS010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660818"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFQ35_12765"
     CDS             <1..888
                     /locus_tag="VFQ35_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931561.1"
                     /translation="PDLAVTILGEEGTGKALVAQAIHELSETEGDFVRVDCASIPEHL
                     FERELFGEEGSDGSSGDPPPHGLFSAAGRGTIFLDEIAEIPSSVQSTLLRALECRSYR
                     RVGGSREVPLEARVLSATSRRPTAQDSHIDQDLYFRLAGFTIVTPALRERKEDIPALA
                     RRCLHEFSERYPGTPKVLGERAIEALTSYDWPGNIRELKSVMQQAAVLTKGPTLELEA
                     IETVLRERSGDSLAPSANAAQNSMIAPRQTRESLPVLQRRAIEEAWAASGHNLSAAAR
                     TLGLPRTTLRDRLKRYGLR"
     gene            1145..3070
                     /locus_tag="VFQ35_12770"
     CDS             1145..3070
                     /locus_tag="VFQ35_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9931562.1"
                     /translation="MGSHEDRRDGTAIETTRSKSRPGRPVEAGFEPPLEPGDLLVGKY
                     RIEELIGTGGIGFVMSATNVGLDEKVALKFLRPEFLSSEEAVHRFTNEARLAAKIQHP
                     NVARVLDIENADELGPFIVMELLVGRDLNQILQHEGPMPVARAVDYVLEACAALSAAH
                     ARDIVHRDVKPDNLFLARQQQGPDLLKILDFGISKQTLRRAESGRSSVLYRTITGLGS
                     PSYMSPEQIRACPDIDGRADVWSLGCVLYELLTARHAFDAPTLMQVCAVVLEKDPPAV
                     RRHAKEVPSELEAVIRRCLQKAPADRFQTVDELARALRPFSSRGANDEERAPDSGRPT
                     PRRLALDPSGQSGEFEPTSVGSIAPASTAPVALNAAPARAVAQPRARRIHFAALTPRQ
                     AFACAAVAAGFGVLAATYRATRAPARFVPPVAAAARPELPELQATVTPFALESLPVSS
                     CPAPMQSASTARERRDVPKPTSSAALPKSPEVTLPAHATVHVPPSEEVPTPPSSNFTE
                     AIQPESAPASTASSSITLPRPEPVARPVEHIAAPKPAAASRLAPSAIAAVVRAHGSEL
                     QNCFERAQLDFDIAKGKLAIVATLDREGHVIDARAALPDADHARLGLCLTTAAKRWKF
                     PSNASAQPFTQYRLLFE"
     gene            complement(3186..3983)
                     /locus_tag="VFQ35_12775"
     CDS             complement(3186..3983)
                     /locus_tag="VFQ35_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9931563.1"
                     /translation="MPAERYKFEAEELSRVFRHGRKHAVAVDRVSFKIKDRDIVSLVG
                     QSGCGKTVLAKMLLRLEAPSAGSLLFDGKPIDAEKDPRAHWRRVQAVFQDPFSCFNQF
                     FTIRSQLKSSFHLFEQKPSEAEMEERVDRALLAVNIKPKEIEGKYPFELSGGQMQRML
                     LARIFILRPEVLIADEPTSMVDACSRANILDYLMKLKEELQMTIVFVTHDIGLAYYIS
                     DAIYIMHQGRIVEQGTPDQVTQSPTSDVTRQLLEDIPNIHHDWLRRA"
     gene            complement(3973..>4662)
                     /locus_tag="VFQ35_12780"
     CDS             complement(3973..>4662)
                     /locus_tag="VFQ35_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931564.1"
                     /translation="MIPQGALNSLNPTRKVKDLAADMILSHDESAERRGIYDRLRERF
                     GRIGLAADEVLNSYPVQLPAGMKQRVVIGISTLLNPKVVIADEPTSALDVSTQKAVIR
                     LLFDLMDQGIISSMIFITHELPLLRHVATSIAVMYAGEFVEVGTTEQVIFDPRQPYTR
                     ALMSSMLSAEVGQRGKKPIAIEGAPPNLAKKISGCRFAERCPEARPDCSTREQELRLV
                     AERKVRCQYAG"
BASE COUNT          848 a         1510 c         1488 g          816 t
ORIGIN      
        1 cccgatttgg cagtgaccat tttgggagag gagggcaccg gcaaagcgct cgtggcgcag
       61 gccattcacg agctcagcga gaccgaggga gatttcgtgc gcgtggactg tgcgtcgatc
      121 cccgagcatc tgttcgagcg cgagctcttc ggtgaagagg gcagcgacgg ttcctcgggc
      181 gatccaccgc ctcacgggtt gttctcggcg gctggacgcg gaacgatctt cttggatgaa
      241 atcgcggaaa ttccgagctc ggtgcagagc acgctcttgc gcgcattgga gtgccgttcg
      301 tatcggcgcg ttggcggcag ccgcgaagtc ccgctcgaag cgcgcgtgct gtcggcgacc
      361 agccggcgac ctacggcgca agactcacac atcgatcagg atttatattt ccggctcgct
      421 ggctttacga tcgtgacgcc agcgctccgc gagcgcaaag aagacatccc cgctctcgct
      481 cgccgttgtc tgcacgagtt cagcgagcgt tatcccggaa cacccaaagt gctcggagag
      541 cgcgccatcg aagcgctgac tagctacgat tggccaggca acatccgcga actcaagtcg
      601 gtgatgcagc aagccgctgt gctcaccaag ggccctacgt tagagctcga agcgattgag
      661 acggtgctgc gcgagcggag cggagactca ctcgcaccga gcgcgaacgc agctcaaaac
      721 tcgatgatcg cgccgcgaca gacacgtgag tcgctgccgg tcttgcagcg ccgagcaatc
      781 gaagaagcgt gggcggcgtc ggggcacaac ctgagcgcgg cggcgaggac gctgggcttg
      841 ccccgcacta cgttgcgcga tcgattgaag cggtacggac tccgctgacc gaccggtcat
      901 cgtcactcgc agtgtggcga tcccgccggc gtgagagaaa ctcacgatgt tctgcgagga
      961 agactccgcg cgcgcgggtg acattgaggc gcgcgcgggc gaaatccgtc ggcgacgttc
     1021 agtcacgttg gaccggcccg cgtgagcgga cttttccgcg cttgccgcac tcgaagagtg
     1081 cctgtaggag gaccctgagc gttctgactg cggcaattct ttggcgcgcg gaggagacga
     1141 acccatgggc tctcacgaag accgacgcga cggcaccgct atcgaaacga ctcgaagcaa
     1201 gtctagacca ggaagaccgg tcgaagcagg cttcgaaccc ccactcgaac cgggcgattt
     1261 gctggtcggc aagtaccgca tcgaggagct gatcggcacg ggcggcatcg ggttcgtgat
     1321 gtcggcgacg aacgtcgggc tagacgagaa ggtcgcgctg aagttcctgc gccccgagtt
     1381 tttgagcagc gaagaggccg tgcatcgctt cacgaacgaa gcgcgcctgg ccgccaagat
     1441 tcagcatccg aacgtcgcgc gcgtgctcga tatcgaaaac gccgacgagc tcgggccctt
     1501 catcgtcatg gagcttttgg tgggccgcga tctgaatcag atcctccagc acgaggggcc
     1561 catgcccgtc gcccgggcgg tcgattacgt gctggaggct tgcgcagccc tgtccgcggc
     1621 gcacgcacgc gacatcgttc accgcgacgt aaagcccgac aatttgttct tggcccgtca
     1681 acagcaagga cccgacctcc tcaagatctt ggacttcggg atctcgaagc aaacgctgag
     1741 gcgcgccgag agcggccgca gctcggtcct ataccgaacc atcaccgggc tcggctcacc
     1801 ctcgtatatg tcgcccgagc agatccgcgc ctgcccggac atcgatgggc gggcagacgt
     1861 ttggtcactc ggttgcgtgt tgtacgagct cttgaccgcg cgtcacgcgt tcgacgcacc
     1921 gacgctgatg caggtgtgcg cggtggtcct agagaaggat ccaccggccg tgcggcgcca
     1981 cgccaaagaa gtgcccagcg agctcgaagc ggtgatacgt cgctgcctgc agaaggcgcc
     2041 agcggatcga tttcaaacgg tcgacgagct cgcccgggcg ttgcgcccgt tttcgtctcg
     2101 cggcgcgaac gacgaggagc gcgcaccgga ttcgggccgg ccgacaccac gacggctggc
     2161 gctcgacccg tccggccaga gcggcgaatt cgaaccgact tcggtcggct caattgcgcc
     2221 cgcgtcgact gcgcccgtgg cattgaatgc agctcccgcg cgagctgtcg cgcagccgcg
     2281 cgctcggcgc attcacttcg cggcactgac gccgcgacaa gcgtttgcgt gcgccgccgt
     2341 cgcggctggt ttcggcgtgc ttgcggcgac gtaccgagcc acgcgggccc ccgcgcgttt
     2401 cgtgccgcca gtcgcggcgg cagcgcgacc cgagcttccg gaattgcaag cgacggttac
     2461 cccgttcgcg ctcgaatcgc tgccggtcag cagctgccca gcaccgatgc aaagcgcttc
     2521 cacggcccgt gagcgccgag acgtaccgaa gccaacgtcg agcgctgcgc tgccgaagag
     2581 ccccgaagtg acgcttcccg cgcacgctac ggtacacgtc ccgccatcgg aggaagtgcc
     2641 aacgccgcct tcttccaact tcacggaagc catccagcca gaaagcgctc cggcctcgac
     2701 ggcgagctct tcgatcacgc taccgcgacc ggagcccgtc gcgcgtccgg tcgaacacat
     2761 cgcggcgcca aagcctgcgg ccgcgagccg gctcgcgcca agcgcgatcg ccgccgtcgt
     2821 gcgagcgcac ggcagcgagc tccaaaactg cttcgaacgt gcgcagctcg acttcgacat
     2881 cgccaagggc aagctcgcca tcgtggcgac gctggaccgt gaaggccacg tcatcgacgc
     2941 gcgcgccgcc ctccccgacg ccgatcacgc gcggctcggc ttgtgcctga cgactgcggc
     3001 caagcgttgg aagttcccaa gcaacgcgag cgctcagccc ttcacgcagt atcgcttgct
     3061 cttcgagtga cgccgcgggc gctcagggcc gagccagcgc cacgggcgcg atgccgcgat
     3121 ggacaacctg ctcgattcca cgaatgccgg gacgcttgag ggtcggcgag cttcgcgaag
     3181 ctgggtcagg cgcgacgcag ccaatcgtgg tggatattcg gaatatcctc gagcaactgt
     3241 ctggtgacgt cgctcgtcgg cgattgcgtg acctggtccg gcgtcccctg ctccacgatt
     3301 cggccttggt gcatgatgta aatcgcatcc gagatgtaat acgcaaggcc gatatcgtgt
     3361 gtgacgaaca cgatcgtcat ttggagctct tctttgagct tcatcaagta gtccaggatg
     3421 ttggcgcggc tacaggcgtc caccatcgac gtcggttcgt ccgcaatgag cacctcggga
     3481 cgcagaatga agatgcgagc gagcaacatt cgctgcatct gcccccccga caactcgaac
     3541 gggtactttc cctcgatttc cttgggcttg atgttcacgg cgaggagagc ccgatcgact
     3601 cgctcctcca tctccgcttc gctcggcttc tgctcgaaga ggtgaaatga gctcttcagt
     3661 tgtgagcgaa tcgtgaaaaa ctggttgaag caggagaacg gatcttgaaa taccgcctgc
     3721 acgcggcgcc aatgagcgcg cggatccttc tcggcgtcga tcggctttcc gtcgaagagc
     3781 agcgagccgg cgctgggagc ctcgaggcgc agcaacatct tcgcgaggac ggtcttgccg
     3841 cacccagatt gtccgaccag gctgacgata tcgcggtcct tgattttgaa gctgactcga
     3901 tcgacagcga ccgcgtgctt gcgcccatgc ctgaacacgc gcgagagctc ctcggcttcg
     3961 aacttgtagc gctcagccgg catactggca ccgcaccttt cgctccgcga ccaggcggag
     4021 ctcttgctcg cgggtcgagc aatcgggcct cgcctcgggg cagcgctccg cgaagcgaca
     4081 gccggatatc ttcttcgcga ggttcggggg cgcgccttcg atcgcgatcg gcttcttgcc
     4141 gcgctgcccg acttccgcgc tcagcatcga gctcatgagc gcgcgcgtgt agggctggcg
     4201 cgggtcgaag atgacctgtt cggtcgtgcc cacctctacg aactcgcccg cgtacatcac
     4261 cgcgatggag gttgcgacgt gtcgtaacag cggcagctcg tgcgtgatga agatcatgct
     4321 cgagatgatg ccctgatcca tcaagtcgaa aagcaggcga atcactgcct tctgcgtcga
     4381 cacgtcgagc gccgaggtcg gttcgtcggc gatgacgacc ttgggattca agagcgtcga
     4441 aatgccgatg accacgcgct gcttcatccc cgctggaagc tggacggggt agctgttcaa
     4501 gacctcgtcc gcggcgagcc cgatccgccc gaagcgttcg cgcaagcggt cgtagatgcc
     4561 gcgccgctcg gccgactcgt catgactcaa gatcatgtcc gcggcgagat ccttgacctt
     4621 gcgggtcgga ttcagcgagt tgagcgcgcc ctgcgggatc at
//
LOCUS       DASTKS010000617         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_991174, whole genome shotgun sequence.
ACCESSION   DASTKS010000617 DASTKS010000000
VERSION     DASTKS010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991174"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1338)
                     /locus_tag="VFQ35_12785"
     CDS             complement(169..1338)
                     /locus_tag="VFQ35_12785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET9931565.1"
                     /translation="MADTGERSPDERWSNEFLDSMRQVGDTEADGVIRTLFQQGDVDA
                     VNSLMATLVRNDGLPSALLPEVVRDYLADNARITFDDTAQIARAQNLFALNGPEMLVA
                     LGFYSLPASYAAKKGVQVLYRTAFLKDRPVRRVFETTQMVIDVLTPSGLAAGGRGRVT
                     VDKVRLMHAAVRHLLLTDRALPWDTASLGYPVNQEDLAGTLMTFSYLVLDGLHRLGID
                     VPSEDQEAYLAFWVSLARLLGLRSELYPRNVSEARALTDIIYTRQIAPSMEGVQLTRA
                     LLDGMEGLIPIQHLKSLPAALVQFFLAKDAIGGQNVAALLQVPEEKLPLLAVRILGEL
                     GEISQRFGSDAIGSVIRFFSGHFLQGMLDVSRGGARSPFFIPDALKERWGMPGNN"
     gene            1720..>2536
                     /locus_tag="VFQ35_12790"
     CDS             1720..>2536
                     /locus_tag="VFQ35_12790"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9931566.1"
                     /translation="MNDRTKPLFVSTAIPYVNAAPHVGHAFELVLGDALSRHHRQRGR
                     AVFLSGGTDDHSLKNARAAAERGVSTAALVAEHAAIYRRLHRALDIELDGWLRTSNDP
                     RHLPAVTELWNRCAAAGDLYQDEYSGLYCTGCEAFVSEAELVQGGCATHRDPLERVQE
                     KNWFFRLSRYESALLAALESRTLRVEPRERHNEVLSFVRSGLRDFSVSRSHSRARGWG
                     LPVPGDPSQVIYVWFDALTNYLSVLGHPESTQDFERFWRGPCDREHLIGKDVLR"
BASE COUNT          495 a          852 c          756 g          433 t
ORIGIN      
        1 cgaagctctt gttcttcacc ccgtaagtga cggccgcgat gtagagcaac acccagcacc
       61 cgcctccgcc gagacctatc aggttcggca gcgtccacgg gtcgacgcct tcgaagcgcg
      121 agctaccgat caggctgaag ccgggaccaa ggttggagaa gcccagcatc agttgttccc
      181 tggcatgccc cagcgctcct tgagagcatc cggaatgaag aacggcgaac gcgcgcctcc
      241 ccgcgaaacg tcgagcattc cttgcaaaaa gtgaccgctg aagaaccgaa tcacggagcc
      301 tatcgcgtcg ctgccgaagc gctggctgat ctcaccgagc tcgccgagga tccgcaccgc
      361 gagcaggggc agcttctcct ccggcacctg caagagcgcg gccacgtttt gcccaccgat
      421 cgcgtccttg gccaaaaaga attgaacgag cgccgccggc aagctcttca ggtgctggat
      481 ggggatgagg ccctccattc cgtcgagcag cgcccgagtg agctgaaccc cctccatcga
      541 gggcgcgatt tgccgcgtgt agatgatatc ggtgagcgct cgcgcctcgc tcacgttgcg
      601 tggataaagc tcgctccgta aaccgagcag ccgcgcgagc gacacccaga atgctagata
      661 cgcctcctga tcctccgagg gcacgtcgat gccgaggcgg tgcagcccat cgagaaccaa
      721 gtagctgaaa gtcatcaaag tgcccgccag gtcttcttga ttcaccgggt aaccgagcga
      781 cgcggtatcc caaggcagcg cgcgatccgt cagcaaaagg tgacgtaccg ctgcatgcat
      841 gaggcgcact ttgtcgaccg tgacgcgccc gcggccgccc gccgcgagac cgctgggcgt
      901 cagcacgtcg atgaccattt gcgtcgtctc gaacacgcga cgcacgggtc gatccttcaa
      961 gaaggcggtg cgatacagga cctgaacgcc cttcttcgcc gcgtacgagg ccggcaacga
     1021 gtagaagccg agcgcaacga gcatttcggg cccattcaag gcgaacaggt tctgcgcgcg
     1081 ggcgatttga gccgtgtcgt cgaacgttat gcgcgcgttg tccgcgaggt agtcgcgcac
     1141 cacttccggc aacaaggccg acggcaagcc gtcgtttcgc accaaagtcg ccatcaacga
     1201 gttgaccgcg tcgacgtcac cctgctgaaa cagcgtgcga attacgccat ctgcttccgt
     1261 gtccccgact tggcgcatcg agtccaagaa ttcgttcgac caacgctcgt cgggcgagcg
     1321 ttccccagtg tccgccatct attgctctcc tctgcgtgcg gttgaaaacg caaccgcgct
     1381 tcgaccgaac ctcagatgat agaaagtgcg ttcgcccgcg cgcaatcgcg aacatcgcga
     1441 attcgcgggc agaatgctca cgacatcgcc ttacaaacgc gcccgtgccg aagaggccga
     1501 aggccgaccg agttgacggt cgcacccgag agacttagac tgctcgctac tttcgtcgtg
     1561 acgtctctcc atcgaatgcc gagcatgccc agcgcaacgc gccgggtcgc cgtgtattcg
     1621 atgagctgaa cgcccttcga accaagaaca cgagctgccc gcgggggcct cggtgttcac
     1681 gggccttcgc gcgcgctctc gacgcccgag gaggagccca tgaacgaccg aacaaagccg
     1741 ctatttgtca gcactgcaat cccctacgtc aacgccgcac cgcacgtcgg ccacgctttc
     1801 gagctagtcc ttggtgacgc gctcagccgc catcatcgac agcgcgggcg agccgtattt
     1861 ttgagtgggg ggaccgacga ccacagcctc aagaacgcgc gcgcggcggc agaacgcggc
     1921 gtctcgaccg ccgccctcgt cgcggagcac gcggcaattt atcggcgctt gcaccgcgcg
     1981 ctcgacatcg agctcgatgg ctggctgcgg acgtcgaacg accctcgcca ccttcccgcg
     2041 gtgaccgagc tttggaaccg ctgcgctgcc gccggcgacc tctaccagga cgagtacagc
     2101 ggactttact gcaccggctg cgaggccttc gtcagcgaag ccgagctcgt acaaggcggc
     2161 tgcgccacgc accgcgaccc cctcgaacgc gtgcaggaaa agaactggtt ctttcgcctg
     2221 tcacgctacg aatctgcgct gctcgctgcg ctcgagagcc gaacgctgcg cgtagaaccg
     2281 cgtgagcgac acaacgaagt cctgagcttc gtgcgctcgg gcctccgcga cttcagcgta
     2341 tcccgcagcc attcgcgcgc gcggggttgg ggcttgccag tcccaggaga tccatcgcag
     2401 gtcatctacg tttggttcga cgcgctcacg aactacctct cggtcctggg acatccggag
     2461 tcgacgcaag atttcgagcg tttttggcgc ggtccgtgcg accgggagca tctcatcggc
     2521 aaggacgtgc tgcgct
//
LOCUS       DASTKS010000618         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_768012, whole genome shotgun sequence.
ACCESSION   DASTKS010000618 DASTKS010000000
VERSION     DASTKS010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768012"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="VFQ35_12795"
     CDS             <1..1768
                     /locus_tag="VFQ35_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9931567.1"
                     /translation="CRATAPSERGWTMNINEQASSGPSAPEPSRLDTTLHRLRERAPA
                     FARLSPLKKADLLREVRQRFFEISERMVELGNERKGVSARSSAAGEEWFSGPDISLRS
                     LRLFEASLRDVATRGAPRISEQQRGHTHHGRALVTLTPRDSYDAVVFRGWRAEAWFEA
                     GVEPHDVSRYQASFYRQSAPSGHVVLVLGAGNVGSISVLDVLYHSFVEGGVCLLKMSP
                     VNDYLMPYYERAFAPLIELGYLAITSGDSEIGRYLVSHPAVDAIHVTGSVETHDRIVW
                     GTGADAERRRRENEPLTQKRVTSELGNVSPVLVVPAVYSERDLSSAARSVAGMFVHNG
                     SFNCNAAKLLVTARDWPQREAFLQRLGHVLADVPTRVAYYPGAHERFDRLLTGLDSGR
                     LERFGATVNGALPWVLVRDLEPTSNSPLFRIEPFCSILSEVALPCADTTEFLRTAVPF
                     TNERVWGTLNAMLIAPDRVEKDPALSRELDRALAELRYGTVALNVWPAVGYGLGTPPW
                     GGYPGATLADVQSGLGWGHNALLLERVEKAVLRGPLRLVPPPFWEPGHAHLCDLGRAL
                     AGLEAEPSVSGVARAAWAAFRP"
     gene            complement(1758..>2517)
                     /locus_tag="VFQ35_12800"
     CDS             complement(1758..>2517)
                     /locus_tag="VFQ35_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931568.1"
                     /translation="ARVRGDGRVFLSSRRGAWVLPKYVLGRPLDEVSGAPLPLPRKLR
                     QALAELWYRLAVGDPRRFGLPLPDHRLGDAHPTVSNDLFGLLGGGALSAKPAIVRFQG
                     KRAEFADGSSEELDAVVYATGYRVTFPFFAPEFISAPDNELSLYLRVFHPERPSVYFI
                     GLAQPLGPIMPIAEAQAKLVAAHVSGRYELPSAAEMQRAARRERQAVRARFGSSPRHT
                     MQVDFDEYLESLAAERELGERRPRRARAVREVTA"
BASE COUNT          407 a          831 c          845 g          434 t
ORIGIN      
        1 atgtcgcgcg acggctcctt ccgagagggg atggacgatg aacatcaacg agcaagcatc
       61 ttcggggcct tctgcccccg agccgtcccg actcgatacg acactccatc gtttgcgaga
      121 gcgcgcgccc gccttcgccc gcctgtcacc cctgaagaaa gccgatctct tgcgggaggt
      181 ccggcagcga tttttcgaga tttcggagcg catggtcgag ctcggaaacg agcgtaaggg
      241 ggtatcggcg cgaagctcgg cggcggggga ggagtggttt tccggtcccg acatttccct
      301 gcggtcgctg cgcctcttcg aagcgtcgct gcgcgacgtg gcgactcggg gcgcgccgcg
      361 catctcggaa caacagcgcg gtcatacgca tcatgggcgc gccttagtca cgctgacgcc
      421 gcgcgattcg tacgatgcgg tcgtttttcg cggctggcgc gcggaagcgt ggttcgaagc
      481 gggcgtagag ccccacgatg tctcacggta ccaggcgagc ttctaccggc aatcggcgcc
      541 gagcggacac gtcgtgttgg tgctcggtgc cggcaacgtg ggctccatct cggtgctcga
      601 cgtgctttac cactcgttcg tagagggtgg agtttgcctg ctcaagatga gtccggtgaa
      661 cgactacttg atgccgtatt acgagcgcgc gttcgcgccg ctcatcgagc tcggttatct
      721 ggcaatcacg agcggagaca gcgagatcgg tcggtatctc gtgagtcatc ccgccgtcga
      781 cgcgattcac gtgactggtt ctgtcgaaac gcacgaccgc atcgtgtggg gcacgggcgc
      841 cgacgcggag cgacgccggc gagagaacga gcccctcacc cagaagcgcg tgacgagcga
      901 gctcggcaac gtgagcccgg tgctggtcgt ccccgcggtg tacagcgagc gcgatctctc
      961 gtcggcagcc cgtagcgtgg ccgggatgtt cgtgcacaac ggctccttca actgcaacgc
     1021 ggcaaagctc ctcgtgaccg cgcgcgactg gcctcagcgc gaggcttttc tgcagcgcct
     1081 cggccacgtg ctcgccgacg ttccgacgcg agtcgcgtac tatccgggcg cgcacgagcg
     1141 cttcgaccgc ttgctcaccg gcctcgactc cggaaggctc gagcgcttcg gggcgaccgt
     1201 caacggggcg ttgccgtggg tgctcgtgcg agacctcgag ccgacatcca atagccccct
     1261 gttcaggatc gagccgtttt gctccatcct gagcgaagtc gcgctgccgt gcgccgacac
     1321 gacggagttc ttgcgcaccg ccgttccgtt caccaacgag cgcgtctggg gcactttgaa
     1381 tgccatgctg atcgcccccg accgcgtgga aaaggatcct gccctgtcgc gtgagctcga
     1441 tcgcgccctc gcggagcttc gttatggaac ggtggctctc aacgtttggc ccgcggtggg
     1501 ttacggcctc ggaacccctc cctggggagg ctatcccggg gcgaccctcg cagacgtgca
     1561 gtcagggctc ggctggggcc acaacgcgct cttgctcgag cgcgtcgaaa aagcggttct
     1621 gagggggccg ttgcgactcg tgcccccgcc gttttgggag ccgggccatg cgcatctttg
     1681 tgatctcgga cgagcgctcg caggtctcga agccgagccg agcgtgagcg gtgtggctcg
     1741 cgccgcgtgg gccgccttca ggccgtgacc tcgcggacgg cgcgcgcacg gcgtggacga
     1801 cgctcaccga gttctcgttc cgccgcgagc gactccaggt actcgtcgaa gtccacttgc
     1861 atggtgtggc gcggcgacga accaaagcgc gcccgcaccg cctgccgttc tcgccgcgcg
     1921 gctcgttgca tctcggcggc gctgggcagc tcgtagcggc ccgacacgtg cgcggcgacg
     1981 agctttgcct gcgcttccgc gatgggcatg atggggccga gcggctgcgc gagcccgatg
     2041 aaatacacgg aagggcgctc cgggtgaaag acgcgcaggt acagcgagag ctcgttgtcc
     2101 ggagccgaga tgaactctgg cgcaaagaac ggaaacgtca cccgatagcc ggtcgcgtaa
     2161 acgaccgcgt cgagctcctc gctcgagcca tcggcgaact cggcacgctt tccctggaac
     2221 cgaacgatcg cgggcttcgc cgagagcgcc ccgcccccca acagaccgaa caagtcgttc
     2281 gagacggtcg ggtgggcgtc accgagccgg tgatctggta acggcaagcc aaagcggcgc
     2341 ggatctccga cggctaggcg ataccagagc tcggccaacg cttggcgaag cttgcgcgga
     2401 agcggcagcg gcgctccgct cacctcgtcg agtggccttc cgagtacgta cttgggaagc
     2461 acccaggctc cccgacggct cgacaggaac actcgcccgt cgcctcgcac tcgcgca
//
LOCUS       DASTKS010000619         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1661718, whole genome shotgun sequence.
ACCESSION   DASTKS010000619 DASTKS010000000
VERSION     DASTKS010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661718"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..795
                     /locus_tag="VFQ35_12805"
     CDS             115..795
                     /locus_tag="VFQ35_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931569.1"
                     /translation="MQLLYLGDVGEWHFFEHALATGATGLVERRPLTQTLPVAALVNE
                     HPRRDQCVNELLASIPGLVNEDVNLLRLNVAPACFAATNSSDDATLAVALRTAPLGPD
                     LRERLDAHMKLYFPELGMLGEEVAKRARFAGTCAGCHDLGDSPWSNPSSFSHVSRDLM
                     QFCANSGPDSTRRCYARSPLLNTVFVPHWTDVLDRFLQQPGAYGPLPNGAQSNSAIDG
                     APLARHFR"
     gene            complement(873..2264)
                     /locus_tag="VFQ35_12810"
     CDS             complement(873..2264)
                     /locus_tag="VFQ35_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9931570.1"
                     /translation="MQPVLEFRDVSLKTPTGRALFEGLNVGIGSEHVAVVGRNGVGKS
                     TLLELMAGAISASSGHVQRRGTVHHVEQLEELGEPFSRGELRRRALERARASGADLLL
                     LDEPTLHLDDATVEWLRQWLAAFPGGVVVVSHDRRLLADMRHFFVVSESGCHYFEGSL
                     AALEQHLESEHERTERRYFANLNRLAEEHEHNAQVARGKARKKRRGRCSELDRATPRI
                     RLNQKRDHAQVSHGRLAKLREAKLLALQGWTRATRRALGVELPLELTLPALSRADGNC
                     ALSLQSLTHALHGRVLFEGLELRVCRERVAITGPNGAGKTTLLEIMMGERRADSGVVR
                     VDMSKVGYIAQGGRNWQLDEPLLAHLLTLGLTSDDAAALLVAHKFPLALADRPLRSLS
                     PGERARAAMAAIFARNPSVELLVLDEPTFSLDLVGQRALTRALQLWPGGLVVASHDRE
                     FLEQLRIDRTIAL"
     gene            complement(2264..>3470)
                     /gene="hflX"
                     /locus_tag="VFQ35_12815"
     CDS             complement(2264..>3470)
                     /gene="hflX"
                     /locus_tag="VFQ35_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9931571.1"
                     /translation="PIARAAQLAEILALVQHQGGRVVGQELCDITEPNPRTLLGKGTA
                     QAVAERARGCGATMLVLDAELSPSQTRNLEDATGIAVCDREAVILNVFLRHARTRRAK
                     IQVEIAQLEYLRPRIRGVGLDMDQQTGGNKNARGPGETASELMARKLDGRLKELRSAL
                     ASSETSSMTQRKQRSDCHRIALVGYTNAGKTSLMNALTGAGLAARDRPFETLDTTSRC
                     LTRHGGEIVVSDTVGFIRRLPERLLASFASTLSELTEASLLALVIDLSDPEHELQLLT
                     TLELLEKVRASGVPRFYVWNKVDRLSRPPDEEYLAELSDGHPFMALSSGDAAAVAELE
                     SKLIAAARVREVELDIRVPYAAAQTLSLIYANCRVLDTQANDTGLALRIVGASAAISR
                     IKHSLKKEP"
BASE COUNT          613 a         1154 c         1111 g          592 t
ORIGIN      
        1 ctcggcaacg cgacggccgc ggagagcgcg ccggcttcga ccgagctcgc cgcattggcg
       61 agcagcggct ttcggtcgct gatcagcccg gagcacttcg ggaacgaggg acgcatgcaa
      121 ctactctact tgggtgacgt cggcgaatgg cacttcttcg agcacgcgct cgccaccggt
      181 gcgaccggcc tcgtcgagcg tcgtccgcta acgcaaacgc tgcccgtcgc cgcgctcgtg
      241 aacgagcatc cgcggcgcga ccaatgcgtc aacgagctgc tggcgagcat tccaggcctc
      301 gtgaatgaag acgtgaattt gctgcggttg aacgtcgctc ccgcatgctt cgcggctacg
      361 aactcatcgg acgatgccac tctggccgtg gccttgcgaa ccgctccgtt gggccccgac
      421 ctgcgtgaac ggctcgacgc gcacatgaag ctctactttc ccgagctcgg gatgttggga
      481 gaagaggtcg ccaaacgggc gcgcttcgcc ggaacctgcg cgggttgtca cgacctcggc
      541 gactcgccat ggtcgaaccc gagcagcttt tcccacgtaa gccgggacct aatgcaattc
      601 tgcgcgaaca gcggccccga ctcgacccgc cgttgttacg cgcgctcgcc gctcctgaat
      661 accgtcttcg ttccacactg gacggacgtg ctcgaccgct tcctgcagca gcctggagcc
      721 tatggtcctc tgccgaacgg cgcgcagtcg aacagcgcaa ttgacggcgc gccgctggcc
      781 cggcacttcc gctgaccggg agacgagact cgaccgcgcg agcggcgagc tgtacggcga
      841 gcggaacgca actgcgtcga tccgcgcgcc attcagagcg cgatcgttcg atcgattcgc
      901 aactgttcga gaaactcgcg atcgtggctg gcgacgacga gaccaccagg ccagagctga
      961 agcgcgcgag tcagggcgcg ctggccaacg aggtccaggc tgaaagtcgg ctcgtcgagc
     1021 acgagcaact cgacgctcgg attgcgtgcg aagatggccg ccatggccgc gcgcgcgcgt
     1081 tcgccgggac tgagcgaacg cagcggtcga tccgcaaggg ccaacggaaa cttgtgcgca
     1141 acgagcaatg cggcggcatc gtcgctcgtc aaacccaagg tcagcaaatg agcgagcaga
     1201 ggttcgtcga gctgccaatt ccgtccgcct tgcgcaatgt agccaacctt cgacatgtcc
     1261 acgcgaacga cgcccgaatc ggcgcgacgc tcacccatca tgatttcgag caaggtcgtc
     1321 ttccctgcac cgtttgggcc ggtgatggcg acacgctccc ggcagacccg cagctcgagc
     1381 ccttcgaaga gaacgcgacc gtggagcgcg tgggtgaggc tctggagaga gagcgcgcaa
     1441 ttgccatcag ccctcgacag agccgggagc gttagctcga gcggcagctc gaccccgagc
     1501 gcgcggcgtg tggctcgagt ccatccttgg agcgcgagca gcttggcctc gcgcaatttt
     1561 gccagccggc cgtggctcac ctgggcgtgg tcccgctttt gattcaggcg gatgcgcggc
     1621 gtggcccgat cgagctcgct gcaacgacct cggcgcttct tgcgagcctt accgcgggcg
     1681 acctgcgcgt tgtgctcgtg ctcttcggcg agccggttga ggttagcaaa gtagcgccgc
     1741 tccgtgcgct cgtgctcgct ttcgaggtgc tgctcgagcg ccgctagcga accctcgaaa
     1801 tagtggcagc ccgactcgct gacgacgaag aagtggcgca tgtcggcgag caagcgccgg
     1861 tcgtgagaga cgaccaccac accccccgga aaagcagcga gccactgtcg cagccactcc
     1921 accgtcgcat cgtcgaggtg cagagtcggt tcatcgagca ggagcaggtc ggcgccggat
     1981 gctctggctc gttcgagggc ccgccgtcga agctctccgc ggctgaacgg ctccccgagc
     2041 tcctcgagct gctccacgtg atggaccgta ccgcgccgtt gaacgtggcc ggagctcgcg
     2101 gagattgcgc ccgccatgag ctccagcaac gtggatttgc cgacgccgtt gcgtcccacg
     2161 accgcgacgt gctcgctgcc gatgccgacg ttcaaaccct cgaacaaggc acggccggtc
     2221 ggtgttttga gcgaaacatc acgaaactcg agcactggct gcatcacggt tccttcttca
     2281 agctgtgctt gatgcgcgag atcgccgccg acgcaccgac gatgcgtaac gcgagaccgg
     2341 tgtcgttcgc ctgggtatcg agcacacggc agttggcgta gatcaagctc agcgtctgcg
     2401 ccgcggcgta gggcactcga atgtcgagct ccacctcgcg cacacgcgcg gccgcgatca
     2461 gcttggactc gagctccgca acggccgcgg cgtcgcccga actgagcgcc atgaaaggat
     2521 gcccatcgct cagctcggcc aagtattcct cgtcgggcgg ccgggacagc cgatccactt
     2581 tgttccaaac gtagaaccgt ggcaccccgc ttgccctcac cttctcgagc aattcgagcg
     2641 tcgtcaacag ctgcaactcg tgctccgggt ccgaaaggtc aatcaccagc gcgagcagtg
     2701 acgcttcggt gagctcggac aaagtggaag cgaagcttgc cagcaagcgc tcgggcaagc
     2761 gccgaatgaa gccgacggta tcgctgacga cgatttcgcc tccgtggcga gtgaggcaac
     2821 gcgaggtggt gtcgagcgtt tcgaagggcc tgtcgcgggc cgcgagcccg gcccccgtta
     2881 gcgcgttcat gagcgatgtc ttgccggcgt tagtgtagcc gaccaatgca atgcggtggc
     2941 aatcactgcg ctgtttgcgc tgcgtcatgc tggaggtttc ggagctcgcg agcgcgctgc
     3001 gcagctcctt gaggcggccg tcgagctttc gcgccatgag ctcggaagcg gtttcgccag
     3061 gaccccgcgc gttcttgttt ccgccggtct gctggtccat gtcgagaccc acgccgcgga
     3121 tgcgcggacg caagtactcg agctgcgcaa tttccacttg gatcttggcg cgacgagtgc
     3181 gtgcgtgccg caagaacacg ttcaagatga cggcctcacg gtcgcaaacg gcgatgcccg
     3241 tcgcgtcttc caggttgcgc gtctgcgagg gcgagagctc cgcatccaat accaacatgg
     3301 tcgcaccgca ccctcgagct cgctccgcga cggcctgagc cgtgcccttg ccgagcaaag
     3361 tccgcggatt gggctccgtg atgtcacaca gctcctgccc gaccacccgc ccaccttgat
     3421 gctgaaccag cgccaagatc tcggcgagct gggccgcgcg cgcaatcgga
//
LOCUS       DASTKS010000620         6339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1662037, whole genome shotgun sequence.
ACCESSION   DASTKS010000620 DASTKS010000000
VERSION     DASTKS010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662037"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ35_12820"
     CDS             <1..205
                     /locus_tag="VFQ35_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931572.1"
                     /translation="AEMPKLSHLIAQAEKQLGKDGRVLVRWSGTEPKLRLMLEGPEPD
                     VLKNLVSDMVDAARKDLGSGASA"
     gene            complement(334..1635)
                     /locus_tag="VFQ35_12825"
     CDS             complement(334..1635)
                     /locus_tag="VFQ35_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931573.1"
                     /translation="MTRGRKKTGYAIAWLILMSAPALLTDCGKMPGGPAPNLPGAPEG
                     LPGASNACPANIADAAAIMNANFGLQGELEGKVKAALAAGADLQKIAVDVEGEVTTAC
                     TNLAKDLGVGDDALKPAQDGPGKKAEAACNAASKAIGELKAKAQGKVVVETKPPVCSA
                     SVNAMTDCAAKCDATVKPGSVKVECEGGKLSGKCDAKCEGECTIEGAAKCEGTCSGSC
                     TGECKADFSGKCEGTCNGKCDGKNANGKCAGTCEGKCQGKATGNCSGECKGECSAGCT
                     MKAKGECSGTCTGGCSVEFKEPKCTGEVKPPQMSAECKAHCDAEVKGKLECTPAHVMV
                     RAEGSADAAAAAKLKGALEKNLPALLKVSVGMKKHIGDVAGNVKGSLEGVKAAASAGG
                     MAAVKAGACIAASMEAQAKASVSIEVSVKASASASASAGAG"
     gene            complement(1761..2132)
                     /locus_tag="VFQ35_12830"
     CDS             complement(1761..2132)
                     /locus_tag="VFQ35_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET9931574.1"
                     /translation="MKLPIVLVPHPHVRVDEHVLGGSPHVLGSRVPVRRLWAFYSGGA
                     SVERILKRYPQLGPAKVLDALAFALDNLEVIQADLEREEEILTRAGHRPARKPVSEDQ
                     MELPFAQQSVPTRSTPKRKTK"
     gene            complement(2150..4120)
                     /gene="tkt"
                     /locus_tag="VFQ35_12835"
     CDS             complement(2150..4120)
                     /gene="tkt"
                     /locus_tag="VFQ35_12835"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9931575.1"
                     /translation="MSKFEQAVKTIQFLSVDGVEAANSGHPGTPMALAGMAVDVFTRA
                     LRYNPKDPAWPNRDRFVLSCGHASMLLYSLLHLAGYDLPLDELKHFRQWNSKTPGHPE
                     VGHTVGVETTTGPLGQGVGNAVGLAIASKLAAARVNEPNSELINYRVFCFCSDGDLME
                     GVASEASSIGGHLGLDNLILIYDDNKITIDGSTELSFSEDVGKRYEAYGYFVQHVDGH
                     NPEQFRKALDAALANKGKPSMIVARTIIGIGAPTKAGTSKAHGAPLGKAEVEAAKKAA
                     GWPTEPTFYVPDEARALFAERAAENQKVYDAWYKQKASLPPERAKKLDELLLRAVPEN
                     LYEELIKAIDGKSDATRNTAGKLEQRAAALVPSLVGGAADLAESTKTTIKGSGDIKRG
                     EFGGRNFNFGIREHGMAAIMNGLSLSGFFIPFGSTFLMFADYCRPSIRLAALMHQRCI
                     YVFTHDSVFLGEDGPTHQPIEHLWALRMIPNLDVVRPADGLECAAAWAHAIARDQGPT
                     LIALSRQKLPELTRPAGFDPKQVLQGAYVLSDSPNPEYTLIATGSEVQVALDAKGILE
                     AQGKRVRVVSAPCWNKFESQPQELRDAVLGVGTRRVTIEAGMSGPWRSVVGDKGLTIG
                     IDRFGASAPAERLAEEFGLTGAKVAARVLSEL"
     gene            complement(4134..5282)
                     /gene="mltG"
                     /locus_tag="VFQ35_12840"
     CDS             complement(4134..5282)
                     /gene="mltG"
                     /locus_tag="VFQ35_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9931576.1"
                     /translation="MTPATDQRRKRPSRPTARRKRRYGSLWLGIAVALASALIAGTAT
                     VLIWATLETDAEDRAVFVTFRGAESADETAARLGELGLVSRPALFSIYMGWFAPSVRV
                     AGGEHLLRTGLSPRRILQRLGRLPSRPSSKVSLPEGYTSFQMADRLQERDICGGQAFL
                     KAARDPALLESLGVVGTSAEGYLFPATYELMVDSDPVQVIRQLVAELRKRLTKLDARA
                     NGGMSRLERQRGWKERDVLTLASIIEREAAVKEERPLIASVFLNRLDDPSFRPPGMLQ
                     SDPTAAYGCLAAPTLAPSCAGYTGRVTPEMLRDGANPYNTYRHSGLPPGPIANAGEDA
                     IFAVLSPASTSFLYFVADGRGRHRFSRSFEEHRRAIEQGVLPLGNEQR"
     gene            complement(5279..5701)
                     /gene="ruvX"
                     /locus_tag="VFQ35_12845"
     CDS             complement(5279..5701)
                     /gene="ruvX"
                     /locus_tag="VFQ35_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9931577.1"
                     /translation="MRAAAVDFGSVRVGLAVADEIGLLAHPRPFLDGRDPQGVLRDLK
                     ALVLNEGIDVFVVGLPRRLDGKEGPSARRAREFAGALRKATGVRVVLKDEWLTTREAK
                     GRLREQGLDERAQRGRIDSASAAVLLQSFLDAERGREP"
     gene            5878..>6339
                     /locus_tag="VFQ35_12850"
     CDS             5878..>6339
                     /locus_tag="VFQ35_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9931578.1"
                     /translation="MQFRPVKGMKDILPGDIERWQRLESVFRSTVELYGYSEVRTPIL
                     EALELFVRSTGETTEIVEKQMFEFERSGERLALRPEGTPGSARAFIGGSVHAKEPVTR
                     WYYIGPMFRAEEPQRGRYRQFHQAGCELYGDAGPICDAEMIDMLFCLLRELG"
BASE COUNT         1051 a         2107 c         2033 g         1148 t
ORIGIN      
        1 cgccgagatg ccgaagctct cccacctcat tgcccaagcc gaaaagcagc tcggaaaaga
       61 cggtcgcgtg ctcgtgcgct ggagcggcac cgaaccgaag ctgcgcttga tgctcgaggg
      121 gccggagccg gacgtgttga agaacttggt ttcggacatg gtcgacgccg cccgcaaaga
      181 tctgggctcg ggcgctagcg cctgaatcgc gacgcgacgg ccgccaaata gaaacgggca
      241 ccgaagcgaa cctcggtgcc cgtcttcttt tgtgggggag caacgacgcg tgctgggcag
      301 cgcgctgctg cccagcgttt cgcgaacgtt ggttcagccc gcgccggccg aggcgctcgc
      361 cgaagcgctc gccttcaccg acacctcgat cgagaccgag gccttcgctt gcgcttccat
      421 cgaagccgca atgcaagcgc ccgccttgac cgcggccatg ccgccagcgc tggcagctgc
      481 tttcacgccc tcgagcgagc ccttcacgtt gcccgcgacg tcgccgatgt gcttcttcat
      541 gccgacgctc accttgagga gcgcaggcag gttcttctcg agcgcgccct tcagcttggc
      601 ggccgcggct gcatccgccg aaccttcggc gcgcaccatc acgtgagcgg gcgtgcactc
      661 gagcttgccc ttcacttccg cgtcacagtg agccttgcac tcggcgctca tctgcggggg
      721 cttcacctcg cccgtgcact tcggctcctt gaactcgacg ctgcagccac cggtgcaggt
      781 gccggagcac tcgcccttgg ccttcatggt gcagcccgcg ctgcactcac ccttgcactc
      841 gccgctgcag ttgccggtcg ccttgccctg gcacttgcct tcgcaggttc ccgcgcactt
      901 gccgttcgcg ttcttgccgt cgcacttgcc gttgcaggtg ccttcgcact tgccggagaa
      961 gtctgccttg cactcgccgg tgcagctgcc ggagcaggtg ccttcgcact tggcggcacc
     1021 ttcgatggtg cactcgcctt cacacttggc gtcgcacttg ccgctcagct tgccgccttc
     1081 gcactcgacc ttcacggagc cgggcttcac ggtggcgtcg cacttggcag cgcaatcggt
     1141 catcgcgttc accgaagccg agcaaacggg cggcttcgtt tcgacgacga ctttgccttg
     1201 ggcctttgcc ttcaactcac caatcgcctt gctcgcggcg ttgcaagcgg cctcggcctt
     1261 cttgcccggt ccgtcttgag caggcttgag cgcgtcgtca ccaaccccga gatctttcgc
     1321 gaggttcgta caggcagtcg tgacttcgcc ttccacgtcg acggcgatct tctgcaggtc
     1381 ggcaccggca gccaatgcgg ctttcacctt gccttcgagc tcaccctgca gaccgaagtt
     1441 cgcattcatg atcgcggctg catccgcgat gttggcagga cacgcgttgg acgcgcccgg
     1501 aagcccttcc ggtgctcccg gcaggttcgg agcggggccg cctggcatct tgccgcagtc
     1561 ggtcaagaga gccggcgccg acatcaaaat aagccaagcg atcgcgtatc ccgtcttctt
     1621 acgacctcgg gtcatcgttt cgattcctcc taagcggcca cccgggccgc aatcgtgcga
     1681 gacgctacac ccaagtcgtg tcccgagaaa atacttcggc acaccgaggt cgaccaaccg
     1741 acctaaaatc ccccgtacag ttacttggtt ttccgcttcg gcgtggagcg agtcggcaca
     1801 gattgctgcg caaacggcag ttccatctga tcctcggaca ctggcttacg agccggacgg
     1861 tgccccgcgc gcgttaaaat ttcttcttcg cgttcgaggt cggcctgaat cacctcgagg
     1921 ttgtccagag cgaacgccag cgcatcgagc accttggccg gaccgagctg tgggtagcgc
     1981 ttcaagatgc gctccacgct ggcccccccc gaataaaagg cccaaaggcg tctgaccggc
     2041 acgcgcgagc cgagcacgtg cgggctaccg cccaacacgt gctcgtcgac gcgaacgtgc
     2101 ggatgaggaa cgaggactat cgggagcttc atggccgcgc gaggcgcgat tacagctcgg
     2161 agaggacgcg agcggcaact ttggcgcccg tcagcccgaa ttcttcggcg agccgctcgg
     2221 ccggcgccga agcgccgaag cgatcgatac caatggtcag gcccttgtca ccgaccaccg
     2281 agcgccacgg tccgctcatg ccggcttcga tcgtcacgcg gcgcgttccg accccgagca
     2341 cggcgtcgcg caactcttgg ggttgactct cgaacttgtt ccagcacggg gcggacacga
     2401 cgcgcacgcg cttgccctgc gcttcgagga tgcccttggc gtcgagggcg acttgcacct
     2461 cgctgccagt cgcgatcagc gtgtactcgg ggtttgggct atcggagagc acgtaggcgc
     2521 cttgcagaac ttgcttcggg tcgaagccgg cggggcgcgt gagctcgggc agcttttggc
     2581 ggctgagcgc gatgagagtc ggaccttgat cgcgcgcgat cgcgtgagcc caagcggcgg
     2641 cgcactcgag gccatccgcg gggcgaacga cgtcgaggtt cgggatcatg cggagagccc
     2701 acaaatgctc gatcggctgg tgggtggggc cgtcttcgcc gagaaacacg gagtcgtgag
     2761 tgaacacgta gatgcagcgt tggtgcatca aagccgccaa acggatgctc gggcggcagt
     2821 agtcggcgaa catgaggaag gtcgaaccga acgggatgaa gaacccgctc aaggagagac
     2881 cgttcatgat cgcggccatg ccgtgctcgc ggatgccgaa gttgaagttt cggccgccaa
     2941 actcaccgcg cttgatgtcg ccggagccct tgatggtcgt cttggtcgac tcggccaaat
     3001 cggcggcacc gccgacgagc gaaggcacga gcgcagcggc gcgttgctcg agcttccctg
     3061 ccgtattgcg cgttgcgtcg gacttgccat cgatcgcctt tatgagctcc tcgtacaggt
     3121 tctcggggac cgcgcgcagc aagagctcgt cgagcttttt ggctcgttcc ggcggaaggc
     3181 tcgccttctg cttgtaccag gcgtcgtaaa ccttctggtt ttcggcggcc cgctccgcga
     3241 acagggcgcg ggcttcgtcg ggcacgtaga aggtgggctc agtgggccac cccgctgcct
     3301 tctttgcggc ctcgacctcg gcctttccga gcggcgcgcc gtgggccttc gacgtgccag
     3361 ccttggtagg cgctccgatg ccgatgatcg tgcgagcgac gatcatcgag ggcttgccct
     3421 tgttcgcgag cgccgcgtcg agcgccttgc ggaactgctc cgggttgtga ccatcgacgt
     3481 gctgcacgaa gtagccgtac gcctcgtagc gtttgccgac gtcttcgctg aacgagagct
     3541 cggtagaacc atcgatcgtg atcttgttgt cgtcgtagat caggatcagg ttatcgagac
     3601 cgaggtgccc accgatgctc gacgcctcgc tcgcgacgcc ttccatcaag tcgccgtcgg
     3661 agcagaagca aaagacacgg tagttgatga gctccgagtt cggctcgttc acgcgcgcgg
     3721 cggcgagctt gctcgcgatc gcgaggccca ccgcgttacc gacgccctga ccgagcggtc
     3781 cggtcgtggt ttcgacgccg accgtgtgcc cgacttcggg gtgccctggc gtcttcgagt
     3841 tccactgacg gaagtgcttc agctcgtcga gcggcaggtc gtaaccggcc aggtggagca
     3901 acgagtagag caacatcgaa gcgtgcccgc acgagagaac gaagcggtcg cggttcggcc
     3961 aagccgggtc cttcgggttg taacgaaggg cgcgcgtgaa gacatcgacc gccatcccgg
     4021 cgagcgccat cggagtaccg gggtgacccg agttggccgc ctcgaccccg tcgaccgaca
     4081 agaactgaat tgtcttaact gcttgttcaa atttgctcat gactgggcct ggtctagcgc
     4141 tgctcgttcc cgaggggaag cacgccctgt tcaatcgcgc gccggtgttc ctcgaagctg
     4201 cggctgaatc gatgacgtcc acgaccgtcg gccacgaagt agagaaaact cgtgctcgcg
     4261 gggctcagaa ccgcaaagat cgcgtcctcg cccgcgttgg cgattgggcc tggtggcagt
     4321 cccgaatgtc ggtacgtgtt gtaggggttc gccccgtcgc gcagcatttc tggggttacc
     4381 ctgccggtgt acccggcgca cgagggcgcg agggtcggtg ccgcgaggca accgtaggca
     4441 gcggtgggat cggactgaag catccccgga gggcgaaagc tgggatcgtc caggcggttc
     4501 aggaacacgc tcgcaatgag cggtcgttcc tctttcacgg ccgcctcgcg ctcgatgatc
     4561 gaggcaagag tgaggacgtc gcgctccttc cagccacgct gccgctcgag ccgcgacatc
     4621 ccgccgtttg ctcgtgcgtc gagcttggtg agtcgcttgc gtagctccgc caccagctgg
     4681 cgaatgacct gaactgggtc cgaatcgacc atgagctcgt aggtcgccgg aaacaaatag
     4741 ccctcggcgc tcgtgccgac cacgccgagg ctctcgagca gcgccgggtc gcgcgctgcc
     4801 ttcaagaagg cttgccctcc gcagatgtcg cgttcctgca gtcgatccgc catctgaaac
     4861 gacgtgtagc cttcgggcag cgacactttg gagctagggc gcgacggcaa ccgtccgagg
     4921 cgctgcagaa tccgccgcgg tgaaagaccg gtacggagca gatgctcgcc gccggccacg
     4981 cgcacgctcg gagcaaacca gcccatgtag atggagaaga gcgccgggcg agagacgagg
     5041 ccgagctctc cgaggcgggc cgcggtttcg tcggcgctct ccgctccgcg aaacgtcacg
     5101 aatacggcgc ggtcctccgc gtccgtctcg agcgtcgccc aaatcagtac ggtggcggtg
     5161 ccggcgatga gcgcggacgc cagcgcaacc gcgatgccga gccagagcga tccgtaccga
     5221 cgcttccggc gagccgtcgg gcgactcggt cgcttccggc gctggtccgt cgcgggggtc
     5281 acggctcccg tcctcgctcc gcgtcgagga agctctgcaa cagcacggca gcggaggcgc
     5341 tgtcgatgcg tccgcgctgc gcgcgctcgt cgagcccctg ctcgcgcagg cgtcccttcg
     5401 cctcacgcgt cgtgagccac tcgtctttca acacgacgcg gacgccggtc gctttgcgga
     5461 gcgcgccggc aaactctcgc gcccgccgcg ccgacggccc ttccttcccg tccaagcgtc
     5521 gcggcagacc gaccacgaag acgtcgatgc cttcgttgag aaccagcgcc ttgaggtccc
     5581 tcaggactcc ctgcggatcg cggccatcta gaaacggtcg aggatgggcc agcagaccga
     5641 tttcgtccgc gacggccaat ccaacgcgca cagaaccgaa atcgacggcc gcggcacgca
     5701 tggcggcctc gatctagcac ggaccgcgca ttttcattcg ggtttggcgg gccgaaccgt
     5761 caccgcgacg agcctccgag accccctcca ttgtatcgcg ccgcttgacc cgaccccatc
     5821 caccgagcat aagcggtgcg cgcggcatcg ggccgcggcg tttacctcgg aaaacccatg
     5881 cagtttcgcc cggtcaaggg gatgaaggac atcctccccg gagacatcga gcgctggcag
     5941 cgcttggaga gcgtgtttcg cagcaccgtc gagctctacg gttactccga ggtgcgcacg
     6001 ccaatcctcg aagcgctgga gctgttcgtt cgctcgaccg gtgaaaccac cgagatcgtc
     6061 gagaagcaaa tgttcgagtt cgagcggagc ggggagcgct tggcgttgcg ccccgagggc
     6121 acccccggct cggcgcgcgc cttcatcggc ggcagtgtgc acgcgaagga gccggtgacg
     6181 cgctggtact acatcggacc gatgttccgc gcggaggaac cgcagcgcgg acgttaccgg
     6241 cagtttcatc aggctggctg tgaactctac ggcgacgcgg gcccgatctg cgacgccgaa
     6301 atgatcgata tgttgttctg cttgctgcgc gagctcggc
//
LOCUS       DASTKS010000621         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_547132, whole genome shotgun sequence.
ACCESSION   DASTKS010000621 DASTKS010000000
VERSION     DASTKS010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547132"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFQ35_12855"
     CDS             <1..1345
                     /locus_tag="VFQ35_12855"
                     /inference="COORDINATES: protein motif:HMM:NF027450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HET9931579.1"
                     /translation="ALLVGASFSTLLREKLRLAELGIPHVLLDSSEGRVARNDAEALR
                     RGGPLVVLVTSEVLGRAEVQSALERSEFGAVAIDSAELAADRGHEVRPSFGLLPARLQ
                     VLGSVSRFAFSRPVPSEVRKQAGERLGFGSTVVLEGALVDPAVRLESRAVRGERRNAT
                     LVELLPSLPERGVILCATPHEVDGLCAVLGKEHGSVCRVHAAMPAADRHVMRERFDAE
                     KGRALLVTTSALGPDVGIPGLGESALSEPRVGFGADTAPGALGFVLHHHAPASLEQYV
                     RELAWLRSADAVSLVFYDSSHRSMNGAILEQARLPSQKLEPFARALESTLAAGRPLKP
                     EALALQAGTSRRTSERMLALWIDAGLARRDKGTIVLEKTPAERAGLVDALARTLQQLE
                     HDDAKRLAAVERYAESNECKRRALAQYFGRSLEPNCGSCSSCRPRAAAERQAHAR"
     gene            1395..2528
                     /locus_tag="VFQ35_12860"
     CDS             1395..2528
                     /locus_tag="VFQ35_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET9931580.1"
                     /translation="MSIPLSTLLEKLESLAPLRYAEEWDNVGLLIDPRARGADLAVRR
                     VMLCIDATPAVVEEAVNARIGCLVAYHPPLFRPQKRLSNADAPLLFAAARHGFAVYSP
                     HTALDAVPEGINDWLVEALPVGAAQALTPYPEHDPNARFKLVVFVPRDHADRLRDALS
                     EAGAGRIGAYSRCSFNITGEGTFFGEPGASPVVGQAGELERVDEERLEMVCSQRSLPA
                     ISATIARHHPYEEPAWDLYPLAPKPDERAGAGRLVTLEQETSLSDIVERFKSHLELPR
                     LRVALSPAHRDGAYVRRVAICAGSGRSVFEKAPEAEVYVTGELGHHDVLALVNRGKSV
                     VLAEHTHTERGYLPRFRERLLSATQNEAEVLLAESDREPLAWI"
BASE COUNT          384 a          903 c          884 g          440 t
ORIGIN      
        1 ggcgctcttg gtgggcgcgt cgttctcgac gttgctccgc gaaaaactcc ggctcgcgga
       61 gctcgggatc ccgcacgtgt tgctcgacag cagcgagggt cgagttgccc gcaacgacgc
      121 agaggcgctt cgccggggcg ggccactggt cgtgctcgtc acgagtgagg tgctcggccg
      181 cgccgaggtt cagagcgcgc tcgagcgcag cgagttcggc gccgtcgcca tcgattcggc
      241 ggagctcgcg gcggatcgcg gccatgaggt gcgcccgtcg tttggcttgc tcccagcgcg
      301 tcttcaggtg ctcggttcgg tgtcccgctt tgcgttttcg cgcccggtgc cgagcgaggt
      361 gcgcaagcaa gcgggggagc ggctcgggtt cgggagcacc gtcgtcctcg agggcgctct
      421 cgtggacccc gcggttcgcc tggagtcccg cgcggtacga ggggagcgcc ggaacgcgac
      481 cctcgtcgag ctcttgccat cgctgcccga gcgcggagtc atcttgtgcg caacgccgca
      541 cgaggtcgac ggcctgtgcg cagtgctggg caaggagcac ggcagtgtgt gccgcgtgca
      601 cgcagccatg cccgccgccg atcgccacgt catgcgcgag cgcttcgacg ccgaaaaggg
      661 tcgcgctttg ctggtcacga cgagcgcgct tggtccggac gtcggcatcc ctgggctcgg
      721 cgagtcagcg ctgagcgagc cccgggtcgg cttcggcgcg gacacggctc ctggcgctct
      781 cggcttcgtc ctgcaccatc atgctccggc ttcgctcgag caatacgtgc gtgagctcgc
      841 ttggctacgc tcggccgatg cggtctcgct ggttttctac gactcatcac accgcagcat
      901 gaacggcgcg atcttggaac aagcgcgcct tccgtcgcaa aaactcgagc cgttcgcgcg
      961 cgccctcgag tcgacgctgg ccgcgggccg ccccttgaag ccagaagcgc tcgcgcttca
     1021 ggcggggacg agccgtcgta cgagcgagcg catgctcgcc ctctggatcg acgcggggct
     1081 cgcgcggcgc gacaaaggca ccatcgtgct cgagaagacc ccggccgagc gggccgggct
     1141 cgtcgatgcg ctcgcacgaa cgctgcagca gctggaacac gacgacgcga agcggctcgc
     1201 cgcagtcgag cggtatgccg aatccaacga gtgcaagcga cgcgcactcg cacagtactt
     1261 cggtcgctcc ctcgagccga actgcggctc gtgcagcagc tgccggcctc gcgcagctgc
     1321 cgagagacag gcccacgcgc gctgacccgt cggccgcggt tgacgcgcgt agcaagcgaa
     1381 gctgagctgt cggcgtgagt atccctctct cgaccttgct cgaaaagctc gagtccctgg
     1441 ccccgctccg ctacgctgaa gagtgggaca acgtcggtct tctgatcgat ccgcgcgcgc
     1501 gcggcgccga tctcgcggtg cgccgagtga tgctgtgcat cgacgcgacc cccgccgtgg
     1561 tcgaggaagc ggtcaacgcg cggatcggct gtctcgtcgc ttaccacccg ccgctgttcc
     1621 ggcctcaaaa gcggctctcc aacgccgacg caccgctgct cttcgcggcc gctcgccacg
     1681 gttttgcggt gtattcgccg cacaccgcgc tcgacgcggt cccggagggc atcaacgatt
     1741 ggctggtcga ggcgttgccc gtcggcgcgg ctcaggccct cactccgtac cccgaacacg
     1801 acccgaacgc gcgcttcaag ctcgtcgtgt tcgtcccgcg cgatcacgcc gaccggcttc
     1861 gcgacgcctt gagcgaggcg ggtgcaggac gcatcggcgc gtattcccgt tgctccttca
     1921 acatcacggg tgaaggcacg tttttcggag aacccggcgc ttctcctgtc gttggccaag
     1981 ccggagagct cgaacgcgtc gacgaggagc gtctcgagat ggtctgctcg caacgcagct
     2041 tgcccgcgat cagcgcgacc atcgcacggc accaccctta cgaggagccg gcgtgggacc
     2101 tttaccccct ggcgccaaag ccggacgagc gcgcgggcgc ggggcgcctc gttacgctcg
     2161 aacaggagac gtcgctcagt gacatcgtgg agcgcttcaa gtcgcacctc gagttgcctc
     2221 gcttgcgcgt cgcgttgtcg cccgcgcatc gggacggcgc ctacgtccgt cgcgtcgcca
     2281 tctgcgccgg ctcggggcga agcgtcttcg aaaaagcgcc cgaagcggag gtctacgtca
     2341 cgggagagct cggccatcat gacgtcctcg ccctcgtaaa tcgcggcaag agcgtcgttc
     2401 tcgccgagca cacccacacc gagcgcggct acttgcctcg ctttcgggaa cggctgctct
     2461 ccgcgaccca aaacgaggca gaggtcctgc tcgccgagtc ggatcgcgag cccctagcct
     2521 ggatctgatc gcgctcgggc ttgggacaga ccgataggca ggcgcctcgg gggtgggccg
     2581 ctagttagag cttgtcgtgg cgattggctt t
//
LOCUS       DASTKS010000622         7009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_547282, whole genome shotgun sequence.
ACCESSION   DASTKS010000622 DASTKS010000000
VERSION     DASTKS010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547282"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1690)
                     /locus_tag="VFQ35_12865"
     CDS             complement(311..1690)
                     /locus_tag="VFQ35_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9931581.1"
                     /translation="MQIKNPATLAIIEEVATDDAASIARKYERARKAQPAWASTPLER
                     RLGVLRAFRDAVAKERDTLALTLTREMGKPVVQAKNELNALSGRLDFFLENTAKVLHP
                     EVVLEERDGTTAKLVESIVFEPLGVIANVSAWNYPWFVGSNVFVPALLTGNTVLYKPS
                     EHATLTGLAIERLLHQSGVPSDAFIAVTGAGNVGAELLAQPVNGVFFTGSYRTGKKIA
                     EQVAARFVRVGLELGGKDPTYVCEDVDVRKAAESLADGAMYNTGQSCCSVERIYVHRN
                     IAPQFLKEFVDVVKSFTIGDPEDEKTYIGPLAREEQLAELELQVKDAVEKGARLLIGG
                     KRIARNGFYFEPTVLTDVSDEMRVMREESFGPIIGIGTVDSDEEAIRRMNDTEYGLTA
                     GVYSRDERRAEAILRELDAGSVYWNCCDRVSPRLPWTGRKHSGVGSTLSTHGIRSFVQ
                     PKAWHRRAP"
     gene            complement(1705..2892)
                     /locus_tag="VFQ35_12870"
     CDS             complement(1705..2892)
                     /locus_tag="VFQ35_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9931582.1"
                     /translation="MPIHQYNFPTKIHYGPGALEMLPDVLKSAGKSRPLIVTDRGLAM
                     LPPVLEASALLKRANLEHTVFSGVWGNPVKSQVSAGVEAFRSHGADAIVGLGGGAALD
                     VAKAIALMAHHPGDLFDYEDEKPGARPIDQPIPYWVAVPTTAGTGSEVGRSTVVSDDV
                     SHVKKIMFSPRLLAERVLADPKLTLALPASVTASTGMDALTHLVEAYLAKGFQPLCDG
                     IALQGVRLVSQSLVRAVDYARRIEGGERELLDDERHLEARGLMLNAAMMGGVAFQKGL
                     GVTHSLAHSLSTVTDMHHGFANGIAIPYAMKFNQSAAAERLADLALLVGASDHSPAGF
                     IRWLEELKAAIGIPRSLSEAGVKSEQLDALVNVAIHDACHPNNPRPVNEADFRGLFQA
                     ALG"
     gene            complement(3012..4382)
                     /locus_tag="VFQ35_12875"
     CDS             complement(3012..4382)
                     /locus_tag="VFQ35_12875"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9931583.1"
                     /translation="MSRARSHAQTVAERVRKSGAQKVKVAVTDIDGVMRGKYIHIDKF
                     LSALESGFGFCNVVFGWDSSDVCYDNAAYTGWHTGYPDANVRLDPNTLRTVPWDNGVP
                     FMLGDFVTETGDPLAVCPRQLLKRVLGELEGAGYAAYSSLEYEFFHFHETPQSLAENA
                     YTEPEPITPGMFGYSLLRLNQNREYVNALMDELLAFGVPIEGLHTETGPGVMEAAIVY
                     SEPLEAADRATLFKTASKEIARRFGIMPTFMAKWNAELPGSSGHIHQSLWDRGLKKNL
                     FFDARADGGVSKLFEHYIAGQLACLPELLPLYAPTVNSYKRLVEGAWAPTKANWGFDN
                     RTCALRVIAGSPKSTRLETRVNGADSNPYLAVAAAFASGLYGIREKLPLAPPVKGNGY
                     ADSKAAVLPRDLWEATQRFKKSKLARAFFGDAFVDHFAASREWEYRQYARAVTNWELK
                     RYFEII"
     gene            4467..5150
                     /locus_tag="VFQ35_12880"
     CDS             4467..5150
                     /locus_tag="VFQ35_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9931584.1"
                     /translation="MPVRLTLPTELDLQEVPVFPLPHVVLLPGALLPLHVFEPRYRLM
                     TRDALAGRRLLAIARLMPGFEADYHGRPPIHSICGVGEIIRHELRADGCYDILMAGLV
                     RAAITEELPATREYRLVRAQALRDRRPLDPLTLGAWQRELVTLWEKLGPYLPTSVRDL
                     CQRANASESIGAWGDRIAAALVADPDARQALLEELDPAERISSLVQRLHELLSALGED
                     RASANIQLN"
     gene            complement(5178..6623)
                     /locus_tag="VFQ35_12885"
     CDS             complement(5178..6623)
                     /locus_tag="VFQ35_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9931585.1"
                     /translation="MRTFKPQVSLAGRKTFSLGIADSIRASIVRGQLRPGELLPSTRE
                     LAAELRVHRHTVLAALEILVSEGRLEAHARRGFSVSQRAQFERSPPTRAAPSFAGFRI
                     VRDGARVDAPPVSAIPYPLHSATPDPTLAPHAELKAAYAHVLRRPRRELFEIADERGH
                     PPFIRVLGDHVRQNRGLVARELMVTHGSQEAIALVARALVAPGDVVAVEEPGYPPAWD
                     AFRAAGARLAAVRVDAQGLDVEALTRLAKRRRVRLLYTTPAHHFPTSVTLSPERRGEL
                     LTITARHGIPILEDDYDHEYQYVGLPVAPLAADSSFGHVIYAGTLSKLIGSGVRLGFV
                     VAAPELLQVLARLCRIGTRGHDPVAQAALAAWMGDGGLERHLRRARRAYVARREALLA
                     AFAEAVSDGSLALQVPNGGLGLWARFRGCDSMKLALAAQQRGITVLPEQSLRLREATC
                     DGARLSFSRCPPDELLAAGAELLQIARELRR"
     gene            6722..>7009
                     /locus_tag="VFQ35_12890"
     CDS             6722..>7009
                     /locus_tag="VFQ35_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931586.1"
                     /translation="MKGIARVLQGAAVRLLPLRAELEPRIVAAALAHPEVWRHIPYPM
                     RTEAEIRERLAQAEVMQSSGAGVVYATEHRETGKIIGGTAIWTVDPRVPSFE"
BASE COUNT         1157 a         2248 c         2359 g         1245 t
ORIGIN      
        1 cggaatttca gctttgctgc ccgtgtgagc ccagctcgga gaagctgacg aaggatgaga
       61 ggggcgcgcc tctgcgcggt ttgccacgac gcgccgcgcc ccgatgaaga atgagacttc
      121 ggcacctgac ggccggccgc ggccccctag cagggcgttg aaaagcgccc tgctagagtg
      181 ccgactggca ccaacgtgcg gctgcgcatc acgaaagccc atgaattgag gctttcgtga
      241 tgcttgcttc tgcgcgtcgc gtgtacctgg cattggtctg tcgtgacgca gggcgttttt
      301 caacgcccgg ctaaggcgct cggcgatgcc acgccttggg ctgaacgaaa ctgcggatgc
      361 cgtgcgtgga gagagtcgag cccacgccgg agtgctttcg tcccgtccac ggcaagcgcg
      421 ggctcacgcg atcgcagcaa ttccagtaga cgctgcccgc gtcgagctcc cgcaagatcg
      481 cttccgcgcg gcgctcgtcg cggctgtaga cgccggcggt gagcccgtat tcggtgtcgt
      541 tcatgcgccg aatcgcctct tcgtccgagt cgaccgtgcc aatgccgatg atggggccga
      601 agctctcttc gcgcatgaca cgcatttcat cgctcacgtc ggtgaggacg gtcggctcga
      661 agtagaagcc gtttcgagcg atgcgcttgc caccgatcaa gaggcgagcg cctttctcga
      721 cggcgtcctt cacttggagc tcgagctcgg cgagttgctc ttcgcgcgcg agcggcccga
      781 tgtacgtctt ttcgtcctcg ggatcgccga tcgtgaaaga cttcaccacg tcgacgaact
      841 ctttcaagaa ttgcggggcg atgttgcgat ggacgtagat gcgctcgacg gagcagcagc
      901 tctgacccgt gttgtacatc gcgccgtccg cgaggctctc ggcggctttc cgcacgtcga
      961 cgtcttcgca gacgtacgtc ggatcttttc caccgagctc gaggccgacg cgcacgaagc
     1021 gcgcggcaac ctgctcggcg attttcttac cggtgcgata actcccggtg aagaagacgc
     1081 cgttcaccgg ctgggccaaa agctcggcgc cgacgttgcc cgcgcccgtg acggcgatga
     1141 aggcgtcgct cggaactccg ctctgatgca acagtctctc gatggcgagc cccgtgagcg
     1201 tggcgtgctc ggacggtttg tagagcacgg tgtttcccgt caaaagagcg ggcacgaaca
     1261 cgttcgatcc gacgaaccag gggtagttcc atgccgatac gttcgcgatc acgccgagcg
     1321 gctcgaacac gatgctttcc acgagcttcg cggtcgtgcc gtcacgctcc tcgagtacga
     1381 cttcgggatg taggactttc gcggtgttct cgaggaagaa atcgagccgc ccgctcagcg
     1441 cgttgagctc gttcttggcc tgaacgacgg gctttcccat ctcgcgcgtg agggtcagag
     1501 cgagggtatc gcgctctttg gcgacggcgt cgcgaaaagc gcgcagcacg ccgaggcggc
     1561 gttcgagggg cgtgctcgcc caggctggct gagccttgcg cgcgcgctcg tacttgcgcg
     1621 cgatgctggc ggcgtcgtcg gtcgcgacct cttcgatgat cgcgagggta gctggatttt
     1681 tgatctgcat gggtgcgccc ttgctcagcc caacgcggcc tgaaacagac cgcgaaagtc
     1741 ggcttcgttc acgggacggg ggttgttggg atgacaggcg tcgtggatcg cgacgttcac
     1801 cagcgcgtcg agctgctcgg acttgacgcc cgcctcggac aagctgcgcg ggatgccgat
     1861 cgcggccttc agctcttcga gccaacggat gaacccggcc gggctgtgat ccgaagcgcc
     1921 caccaagagg gccaagtcgg ccaggcgctc cgccgccgcg ctctggttga acttcatcgc
     1981 gtaggggatg gcgatgccgt tcgcgaagcc gtggtgcatg tccgtgacgg tcgagagcga
     2041 gtgcgcgagc gagtgcgtca cgccgagccc cttttggaag gcgacgccgc ccatcatggc
     2101 cgcgttgagc atcagcccgc gcgcctcgag gtgacgttcg tcgtcgagta gctcgcgctc
     2161 gccgccttcg atgcgacggg cgtagtcgac cgcgcgcacc aggctttggc tcaccaggcg
     2221 cacgccttga agcgcgattc cgtcgcacag cggctgaaaa cctttggcga ggtaggcttc
     2281 gacgaggtgg gtgagcgcgt ccatgccggt cgacgccgtg accgaggcgg gtagcgcgag
     2341 cgtgagcttc ggatccgcca gcacccgctc ggcgagcaag cgcggtgaga acatgatctt
     2401 cttcacgtgc gacacgtcgt ccgaaacgac ggtgctgcgc ccgacctcgc tgccggtgcc
     2461 ggcggtggtg ggcacggcga cccaataggg aatcggctga tcgatggggc gcgcgccggg
     2521 cttttcgtct tcgtagtcga acaagtcacc cgggtggtgc gccatgagag caatggcctt
     2581 ggcgacgtcg agcgcggcgc cgccgccgag cccgacgatg gcgtcggcgc cgtgcgagcg
     2641 aaacgcctcc accccggcgc tgacctgcga cttcacaggg ttcccccaaa ccccagaaaa
     2701 aacggtgtgc tcgaggttgg ctcgcttcaa gagcgcgctc gcttcgagca ccgggggcag
     2761 catcgcgagc ccgcgatcgg tcacgatgag aggccgcgac ttgcctgcgc tcttcaatac
     2821 gtcgggcagc atttcgagcg cgcccggtcc gtaatgaatc ttggtcggga agttgtactg
     2881 atgaatgggc actgtcctct cctcggtttg accatacccg gtaccaggag cccgcacttg
     2941 gcgggcgggc gagctcgagg gtcggtatgt cggtgcgact cacgttcggt gcggcgacca
     3001 ggccgcatcg atcagatgat ttcgaagtaa cgtttcaatt cccaattggt gacggcgcga
     3061 gcgtactgcc ggtactccca ctcgcgagac gccgcgaagt gatcgacgaa ggcgtcgccg
     3121 aaaaaggcgc gcgcgagctt gctcttcttg aagcgctggg tggcctccca taaatcgcgc
     3181 ggtaatacgg ccgccttcga gtcggcgtaa ccgtttcctt tcaccggcgg ggcgagcggg
     3241 agcttctcgc ggatgccgta gaggccgctc gcgaacgcag ctgccacggc gaggtacggg
     3301 ttcgaatccg cgccgttcac ccgagtttcg aggcgagtgc tcttgggact gccagcgatc
     3361 actcgcaggg cacatgttcg gttgtcgaag ccccaattcg ccttcgtcgg tgcccacgcg
     3421 ccttcgacca ggcgtttgta gctgttcacg gtcggcgcgt agaggggcaa gagctccggc
     3481 aggcacgcga gctgacctgc gatgtagtgc tcgaacagct tgctgactcc gccgtcggcg
     3541 cgcgcgtcga agaacaggtt cttcttcagg ccgcgatccc acaagctttg gtggatgtgc
     3601 ccgctcgagc cggggagctc ggcgttccac ttcgccatga acgtcggcat gatgccgaag
     3661 cggcgcgcga tctccttgct cgcggtcttg aagagcgtcg cccgatcggc cgcctcgagc
     3721 ggctccgagt aaacgatggc cgcttccatc acgcccgggc ccgtctcggt gtgcagcccc
     3781 tcgatcggca cgccgaacgc gagcagctcg tccatcagcg catttacgta ctcgcggttc
     3841 tgattcaacc gcaaaagtga gtagccgaac atccccggcg tgatcggctc gggctcggtg
     3901 taagcgttct ctgccagcga ctgaggcgtc tcgtgaaaat gaaagaactc gtactcgagt
     3961 gacgagtacg ccgcgtagcc tgcgccttct agctcgccga gcacgcgctt caagagctgc
     4021 cgcggacaca ccgcgagcgg atcacccgtc tcggtgacga agtcgccgag catgaacggg
     4081 acgccgttgt cccacggaac cgtcctcaac gtattcggat cgagccggac gttggcgtcg
     4141 gggtagccgg tgtgccaccc ggtgtacgca gcgttgtcgt agcaaacgtc ggagctgtcc
     4201 cacccgaaca ccacgttaca aaagccgaat ccggactcga gtgcggaaag gaacttgtcg
     4261 atgtggatgt atttgccgcg catcaccccg tcgatgtcgg tgaccgccac tttcaccttt
     4321 tgcgcacctg atttacggac gcgctcggcc accgtctgag catgggaacg ggctcgactc
     4381 atggtggagc gagtatctgg cgcgcgcgcg ggcggggcaa gtcaccttgt gactcttctt
     4441 ccgccgctct aaactggagg ggtctgatgc cggttcgcct cactctgccc acggagctcg
     4501 accttcaaga ggtaccggtg tttccgcttc cgcacgtggt gttgctgccc ggagcgctcc
     4561 tgccgctcca cgttttcgag ccgcggtatc ggctgatgac gcgcgacgcg ctggccgggc
     4621 ggcgtctctt ggccatcgcg cggctcatgc ccgggttcga ggcggactat cacgggcgcc
     4681 caccgattca ctccatctgc ggtgtgggcg agatcatccg acacgagctc cgcgccgacg
     4741 gctgctacga cattctgatg gcgggtctcg tgcgcgccgc gatcaccgag gagctgccgg
     4801 ccacgcgcga gtatcgtctc gtgcgcgcgc aagccttgcg tgatcggcgc ccgctggatc
     4861 cgctgactct cggagcgtgg cagcgagagc tcgtcacact ttgggagaag ctcggcccct
     4921 acctgccgac ttccgtccgc gacttgtgtc agcgcgcgaa cgcttcggag agcatcggag
     4981 cctggggtga tcgcatcgcg gcagcgctgg tagccgatcc agatgctcgc caggctttgc
     5041 tcgaggagct cgacccggcc gagcgcatct cgagcttggt ccagcggctg cacgagctgc
     5101 tctccgcgtt gggtgaagat cgcgcaagcg cgaacatcca gctcaattga tgccggcctg
     5161 ggctcgcgcc gtaaagctca gcgtcgcagc tcgcgcgcga tttggaggag ctcggcgccc
     5221 gccgcgagga gctcgtcggg tgggcagcgc gaaaacgaaa ggcgcgcccc gtcgcaggtc
     5281 gcttcgcgca agcgcaggct ctgctccggg agcaccgtga tccctcgctg ttgggcagcg
     5341 agcgcgagct tcatgctgtc gcagccccga aagcgtgccc agagcccgag cccgccgttc
     5401 ggcacctgaa gcgcgagtga gccatcggac accgcttcgg cgaacgcggc gagcaaggcc
     5461 tcacgccgtg ccacataggc gcgtcgcgct ctgcgcaaat ggcgctcgag accaccgtcg
     5521 cccatccacg ccgccagcgc ggcttgggct acggggtcgt gaccgcgtgt tccgattcgg
     5581 cagaggcgcg cgagcacttg caagagctcc ggcgctgcga ccacgaaccc gaggcgcact
     5641 ccgcttccga tcagcttgga tagcgtaccc gcgtagatga cgtgtccgaa cgacgagtcc
     5701 gcggcgagcg gcgcgaccgg taagccgacg tactggtatt cgtgatcgta gtcgtcttcg
     5761 aggatgggga tgccgtgtct cgccgtaatg gtgagcagct cgccgcgacg ctcggggctc
     5821 agcgtgaccg aggttggaaa gtggtgcgcc ggggtcgtgt agagcaggcg cactcgacgc
     5881 cgcttcgcga gtcgcgtcag cgcttcgaca tcgagtcctt gcgcatcgac gcgcaccgcg
     5941 gcgaggcgcg cccccgcggc ccgaaacgcg tcccacgccg gcggataacc gggctcttcg
     6001 accgcaacta cgtcaccggg agcgaccaac gcgcgagcga cgagcgcgat cgcctcctgc
     6061 gaaccatgcg tcaccatgag ctcgcgcgcg acgaggccgc gattttggcg cacgtggtcg
     6121 ccgagcacgc ggatgaacgg cgggtgcccc cgctcgtcgg cgatttcgaa gagctcgcgg
     6181 cggggtcgcc gcagaacgtg tgcgtacgcg gccttgagct cggcgtgcgg cgccaaggtg
     6241 ggatccggcg tcgcggaatg gagcgggtag ggaatcgccg agacgggcgg cgcatcgacg
     6301 cgcgctccgt cgcgcacgat gcgaaagccg gcaaacgagg gagcggctcg agtaggcggc
     6361 gagcgctcga actgcgcccg ctggctgacc gagaagccgc gtcgcgcgtg cgcctcgagc
     6421 cgtccctcac tcaccagaat ctcgagcgcc gcgagcacgg tgtgacgatg cacccgaagc
     6481 tcagccgcga gctcgcgcgt cgacgggaga agctcaccgg ggcgaagctg gccccgtacg
     6541 atggaagctc ggatgctgtc ggcgatgccg agggagaagg tcttgcggcc cgcgagcgat
     6601 acttgcggct tgaaggtgcg catgttcggc ggcgtgagcg gcagtttagg cgccgttggt
     6661 cgaaggacaa actgggttct ggcacttttg gttcgaccag aatgcgctca ttgtcccgag
     6721 catgaagggt attgctcgtg tactccaagg cgcagccgta cggctcctcc cgctccgggc
     6781 ggagctcgaa ccgaggatcg tcgcggccgc cctcgctcac ccagaggtct ggcgacacat
     6841 tccctacccg atgcggacgg aggccgagat ccgcgagcgc ctcgctcagg ccgaggtgat
     6901 gcagagctcc ggtgccggcg tcgtttacgc gactgaacac cgggaaacag ggaagatcat
     6961 cggggggacc gcgatctgga ccgtcgatcc gcgggtcccg tcgttcgaa
//
LOCUS       DASTKS010000623         6455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1662508, whole genome shotgun sequence.
ACCESSION   DASTKS010000623 DASTKS010000000
VERSION     DASTKS010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662508"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFQ35_12895"
     CDS             complement(<1..721)
                     /locus_tag="VFQ35_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931587.1"
                     /translation="MGSASGGDSTNVGSASGGVADGGSTNTGGSFDSSSGAGGEPTAG
                     AASGGAPSTTEVKFGFSCNGVADGSLAKWLSSQVIAMAGTWNDTSADVQLEQYTLNHE
                     YRDWNEDIDDAIGGIFKGETWAQAANGDFDVRWRSAVTSIKRAWGSRDPALLNVRFAH
                     EFNLAESQWKVRGDEADAFRDAWARFHGIFKEVLPTASLVWCPNDGTSGSLALDVRKA
                     YPGGSYVDVICIDTYNQWPWVN"
     gene            1607..2914
                     /locus_tag="VFQ35_12900"
     CDS             1607..2914
                     /locus_tag="VFQ35_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931588.1"
                     /translation="MDQILIVEDEAGLRNSLLRCLQTQVGVEVSGVATLRDAVASLDR
                     SRPNLLVLDIDLPDGSGLELLPELSSRDLAIPIVVITAHAKRFQSAFVDQANIEVLSK
                     PFESVEFQRVVFDRLSRTKIEQAAPAFTVSDYLQLAGMARRSVVLSVREGSQKIGEIV
                     IQDGAPRWAEDQQGVGEAAFRRLALHARGDVRCRPLDAPLTKTNLSGSLEQLLIEAAR
                     KADEAKRRGGAAPPPESNRLRAKEPDANSEAPRSSESSRPTLSPVQASNAVQASNAVP
                     TRVPPPPPSAQPRKVPPPKPAGNIGLAGFQVKPSGDEPRPKEKVVTLNKNSKADLNQL
                     LQLDPSIKGAARADQQGSVLESRGELDAETACAVANVALRQIVEATADLGLGRPSAWH
                     VSLGGATWYVAQVRDEIFVARGTLHKNPISTLKKLSKACGAAT"
     gene            2911..3597
                     /locus_tag="VFQ35_12905"
     CDS             2911..3597
                     /locus_tag="VFQ35_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931589.1"
                     /translation="MNAEALSNLSKTDGVLGVVVFDEHGTCLVNQLPAPYEPILLFDV
                     LRRLAGAFDVFASLEDGSAASFSVDCEDGSLVLRCVDGHWVVALTHPEVNMNLLNVAM
                     NVLVLNLSRGFPGSGTQAAVRLQESMTGRSSGSLSISNSMGDQEIPPDAVDRSVVQQL
                     LTIYREFLGPAAKLVFKQQLGALGVTSRTLRRGQVSDLAARLASKIPVADRQKEFLAE
                     VRRFQSRIGI"
     gene            3625..5403
                     /locus_tag="VFQ35_12910"
     CDS             3625..5403
                     /locus_tag="VFQ35_12910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931590.1"
                     /translation="MDSFAPEEDLVRIEHGTAVGNYVVEGFLGQGSEGSVYIANDSIL
                     GRKVALKTLRTGEIGETRAVEEARLLARLEHPHIVRVYHARRYRGIWFVVCEYLGGGS
                     LYAQLQRVGALPTKRALEVAAQAASGLAYAHELGILHRDVKPQNLLIARNGDVKLADF
                     GLAFDTRGVRGASEQPIGTPAFLAPELWSGQAASAASDVFSLGACLFFALTGRAPFVA
                     EDVDRLRRAQLELEPKLPLSLPPAVRELTLGMLAKNPEQRPSMNVALTTLRELAQDPY
                     RVNNPSAEAVRTLPLDPFVSGGPERASASALCQGADFAFVSELAAALKTKPVGIELLA
                     PSLAEALLLCEVARGATSERYELVVRLMLTQPQMSIHDAILRKLGLIGDVPFALACSR
                     LLDSAREGNSAAVIEIGAPRGLTAQQRRELAALSEAAHQRGTVCVVCAPVAQALELAG
                     FRRIEAFSALALVDHEARHAAWIAAATGGRVSFSRDGLRVSGALCREEGRYWPDLLRD
                     SLLIASAARLPVVTSWAILAARSQGGALGSIDDVPAALRRRPVSWPPADVQTLLNRLR
                     KTGEAGFARTPRKETEESCCRPSVAE"
     gene            5400..6200
                     /locus_tag="VFQ35_12915"
     CDS             5400..6200
                     /locus_tag="VFQ35_12915"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HET9931591.1"
                     /translation="MIAILTAFALLLGGVSLATNIVLKRQADDAVVVNLAGRQRMLSQ
                     KMTKEAAQLVNASREQGSEAKFATQREQLQNTLRVFETTLISLRDGGPAPLNLEMTRM
                     RTLPPASTPEIKKQLETVTAVWEPFKKSMNDLIHSNGSDAVAGRAIMDQNLALMDQMN
                     TAVNLMQADSEQKVNVLFVVQTTALGLGLVLVVVGGWMARSTIAKPLIELARAAHSMS
                     TGNLNVELNLSGTQEVKELGASFDRMRASMVAALGGGMGSTNLDDDDL"
     gene            6276..>6455
                     /locus_tag="VFQ35_12920"
     CDS             6276..>6455
                     /locus_tag="VFQ35_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931592.1"
                     /translation="MSGARVCERRECGAQPVGLSLRTHFGTLSMLKAPISLLGRGAAD
                     SRQTLMERIPDLALSE"
BASE COUNT         1125 a         2127 c         2116 g         1087 t
ORIGIN      
        1 cgttgaccca cggccattgg ttgtacgtgt cgatgcagat cacgtcgacg taggaacctc
       61 cgggataggc cttgcgtacg tcgagggcga gcgagccgct cgttccatcg ttcgggcacc
      121 acacgagtga cgcggtcggt aagacttcct tgaagatacc gtgaaagcgt gcccaggcat
      181 cgcgaaacgc gtcggcctcg tctccgcgca ccttccactg gctctccgcc aagttgaatt
      241 cgtgagcgaa gcgcacgttg agcaacgccg gatcccgcga gccccacgcc cgcttgatcg
      301 atgtcaccgc gctccgccag cgcacgtcga agtcgccgtt cgccgcttgt gcccaagtct
      361 cgcccttgaa gatccctccg attgcgtcgt cgatgtcctc gttccaatcg cggtactcgt
      421 gattcaacgt gtactgttcg agctgaacgt cggcgctggt gtcgttccac gtgccggcca
      481 tcgcaatcac ttggctcgat agccacttcg cgagcgaacc gtccgctacg ccgttgcacg
      541 agaacccgaa cttgacctcg gtcgtgctcg gcgcgccccc ggaggcggct cctgcggtcg
      601 gctcgccgcc tgcgccgctc gacgaatcga agcttccgcc cgtattcgtg gagcccccgt
      661 ccgcaacgcc gccgctcgcg gaaccgacgt tcgtcgaatc gccgcccgac gccgagccca
      721 cgggcgtgct ccctccattg gacccactcg agcccggtgt ggtccctccg cctgcgaaat
      781 tggaagcgtg tgaaccgcct gcgtgcgttg cggctccgct cgttccaccc ggtccgccgt
      841 aagtcatcgc accactgctc acgggaggtg cgaccaggcc cgaacctccg ccatgagcct
      901 gagccaaatc cggctctgtc gccgctccct cgcagcggag caacgacaag gcgagcagcg
      961 cgcggggagc gatggaagca cgccgggatc cgggacgcgt gggcagagaa gacatccgta
     1021 cctcgacacc aggctcgtgt tcgcgacgtc ggagcacgag cttcgactcg aggacttaca
     1081 cgttgcgtgc cagcgctgcg tccggagttc gagccgagaa ttcgcgccgt ttcgctgggc
     1141 gagaggcctc ggtacgcaac ccgcagttgc acgagcaact tgcagttgcc tcagtgagcg
     1201 cgagggcaga attctggtcg ctgggcaggc gtgagcctgg tttccggaac tcgggcagcg
     1261 gcggtccgaa ccaacgtcct tcgtgcatga ggacgccgac gctgccaaca cgatttcgag
     1321 ccgtaggtag cgagacatcg tccctcggtc cacatcgtta cacggcgtgt accgccacgt
     1381 tcttccgctg agaagtgcga cctcggccgt cgttgtgagt cgtgtcccga tcgggacggt
     1441 gctgcgccga tccggacgat ctcggcgcgc gtcgtgaccc agtggcgtgt ccattcgagg
     1501 cgttagtgca cggcacgcgg actgcactag caagctccgc cgacttggga tggcgcgccg
     1561 ccactgcgcg ccttcgctca acgaaaccgg ggcggcgtca actgccatgg accaaatcct
     1621 gatcgtcgaa gacgaagccg gtctccgcaa ttcgctgctt cgctgtttgc aaacccaggt
     1681 cggagtggaa gtgagcggag tcgcgactct gcgggacgcc gtcgcctcgc tcgatcgctc
     1741 gcggccgaac ttgctcgtgc tcgacatcga tttgcccgac ggttctggtc tcgagctgct
     1801 gcccgagctc agctcgcgcg acctggcgat tccgatcgtg gtgatcacgg cccacgccaa
     1861 gcgctttcag agcgcgttcg tggatcaggc aaacatcgaa gtgctgtcga agcccttcga
     1921 gtcagtcgag tttcagcggg tcgtattcga tcggctttca cgcactaaga tcgagcaggc
     1981 ggcgccagcg ttcacggttt cggactacct ccagctcgcc ggaatggcgc ggcgcagcgt
     2041 cgttctcagc gtccgagaag gcagccagaa gatcggcgag atcgtgattc aggacggcgc
     2101 gccgcgctgg gcagaggacc agcagggggt gggcgaggca gcgtttagac gactcgcgtt
     2161 gcacgcgcgc ggtgacgtgc gctgccggcc tctcgacgct ccgctgacga agactaacct
     2221 ctcgggaagc ctcgaacagc tcctgatcga ggcggcgcgc aaggcggacg aagccaagcg
     2281 ccgcggcggc gccgctccgc cgccagaatc gaaccggctg cgagcgaaag agcccgacgc
     2341 aaactccgag gctccgcgca gttcggagag ctcgcgcccg acgctctcac ccgttcaagc
     2401 ttccaacgcg gttcaggcgt ccaacgccgt tccgacgcgc gtgccgccgc cgccgccgtc
     2461 cgcgcagccg cgcaaagttc ctcccccaaa gcccgccggg aacatcgggc tcgcggggtt
     2521 ccaagtcaaa ccgtccggcg acgagcccag gccgaaggag aaagtagtga ccctcaacaa
     2581 aaacagcaaa gccgatctga atcagctgct tcagctcgac cccagcatca agggagcggc
     2641 gcgcgccgat caacaaggca gcgtgctcga gtcgcgtggt gagctcgacg ccgaaaccgc
     2701 ctgcgccgtc gcgaacgtcg cgttgcgcca aatcgtcgag gctaccgccg acctcgggct
     2761 cggccgtccc tcggcatggc acgtgtcgct cggcggagcc acttggtacg tcgcgcaagt
     2821 acgagacgag atcttcgtcg cgcgcggcac gctgcacaag aacccgattt cgacgctgaa
     2881 gaagctgtcc aaagcctgcg gagccgccac atgaacgccg aagccctttc gaacctctcc
     2941 aaaaccgatg gcgtgctggg tgtcgtcgtc ttcgacgaac acggcacttg cctcgtcaac
     3001 caactgcccg cgccgtacga gccgatcttg ctcttcgacg tgctgcgacg cctcgcgggc
     3061 gcgttcgacg tattcgcgtc actcgaggac ggaagcgccg cgagcttctc ggtggactgc
     3121 gaagacggca gcctcgtatt gcgctgcgtc gacgggcact gggtcgtcgc gctgacgcac
     3181 ccggaagtga acatgaacct cctcaacgtc gcgatgaacg tgttggttct gaacctgagc
     3241 cgcggctttc cgggtagcgg aacgcaagcg gcggtgcgcc tccaggagtc gatgacgggc
     3301 cgctcgagcg ggagcctttc gatttcgaat tcgatgggcg accaggagat cccgccggat
     3361 gcggtggacc gcagcgtggt gcaacaactg ctcacgattt accgcgagtt cttgggcccg
     3421 gccgcgaaac tcgtgttcaa gcagcagctc ggcgcgctcg gcgtgacctc gcgcacgctc
     3481 cggcgcggtc aggtatcgga tctggcggct cgcctcgcca gcaagatccc cgtcgccgat
     3541 cgccagaaag agttcctcgc cgaagtgcga cgtttccagt cgcggatcgg tatctgaagc
     3601 actcgcgtcc gtcgagcaac acgcatggac tccttcgcac cagaggaaga cctcgtacga
     3661 atcgagcacg ggaccgccgt cggcaactac gtggtcgagg ggttcctagg gcaaggcagc
     3721 gagggctcgg tctacatcgc caacgactcc atcttgggtc gtaaggtcgc gctgaagacc
     3781 ctgcgcacgg gggagatcgg cgagacacgc gcggtcgaag aggcgcgtct cctggcgcgg
     3841 ctcgagcacc cacacatcgt gcgcgtgtac cacgcgcggc gttaccgcgg gatttggttc
     3901 gtcgtttgcg agtacctggg cggcggcagc ttgtacgcgc aactgcagcg cgtgggggcg
     3961 ctaccaacga agcgcgccct ggaggtggcc gcgcaagccg cgagcggcct cgcgtacgcg
     4021 cacgagctcg gtatcttgca ccgcgacgtc aaaccgcaga acctgctcat cgcccgcaac
     4081 ggcgacgtga agctcgcgga tttcggcctc gctttcgaca cgcgtggggt gcggggcgcg
     4141 agtgagcaac caatcgggac gccggcgttc ctggctccgg agctgtggag tggacaggcg
     4201 gcgagcgccg cctccgacgt gttcagcttg ggagcatgct tgttcttcgc gctcacgggc
     4261 cgtgcgccgt tcgtggccga agacgtcgac cgcctgagga gagcgcagct cgagctcgag
     4321 ccgaagctcc ccttgtcgtt gccgccggcg gttcgcgaat tgacgctggg gatgctcgcc
     4381 aagaacccgg agcagcgccc gagcatgaac gtcgcgctga ccacgctgcg cgagctcgcg
     4441 caggatcctt accgcgtcaa caacccgagc gcggaggccg tgcgcacgtt gcctctggac
     4501 ccgttcgtga gcggcggccc ggaacgcgcg tctgccagcg cgctctgcca aggagccgac
     4561 ttcgcgttcg tgagcgaact cgcggccgcg ctcaagacga agcccgtcgg catcgagctc
     4621 ctcgcgccga gcctcgcgga ggcgctgcta ctgtgcgagg tcgcgcgcgg tgcgacttcc
     4681 gagcgctacg agctcgtcgt gcggttgatg ttgacgcaac cacagatgag catccacgac
     4741 gcaatcttgc gcaagctcgg gttgatcggc gacgtgccgt tcgcgctcgc gtgttcgcgc
     4801 ttgctcgact cggcgcgcga gggcaacagc gcggccgtca tcgaaatcgg cgctccgcga
     4861 gggctcaccg cgcagcaacg ccgtgagctc gcggctctga gcgaggccgc gcaccagcgc
     4921 ggaacggtgt gcgtcgtgtg cgcgcccgtc gcgcaagctc tggagctcgc tggttttcgt
     4981 cgcatcgagg cgttctcggc gctcgccttg gtcgatcacg aggcgcggca cgcggcgtgg
     5041 atcgcagccg cgacgggcgg ccgggtgagc ttctcgcgcg acggcttgcg agtctccgga
     5101 gcgctttgca gggaagaggg ccggtactgg ccagacttgc tgcgcgacag cctgctcatc
     5161 gcgagcgctg cgcgccttcc ggtcgtgacc agctgggcga tcctggccgc gcgctcgcaa
     5221 ggcggagcgc tcggctccat cgacgacgtt ccggctgcgc tgcgaaggcg cccggtgagc
     5281 tggccacccg cggacgtcca aacgctcttg aatcgattgc ggaaaaccgg agaggcgggt
     5341 ttcgcgcgca cacctagaaa ggaaacggaa gaatcatgtt gtcgaccatc agtagcagaa
     5401 tgattgcaat tcttacggcg ttcgcgctgt tgttgggtgg agtgtcactc gccaccaaca
     5461 tcgtgttgaa gcgtcaggcc gacgacgcgg tcgtggtcaa cctggctggt cgtcaacgca
     5521 tgctgagcca gaagatgacc aaagaagcgg cccagctggt gaacgcctcg cgcgagcagg
     5581 gcagtgaggc caagttcgcg acgcaacgcg aacaactgca gaacacgctg cgtgtcttcg
     5641 agacgaccct gatttcgctg cgcgacggcg ggcccgcgcc gctgaacttg gagatgactc
     5701 ggatgcggac cctcccgccc gcctcgaccc cggagatcaa gaagcagctc gagacggtga
     5761 cggcggtctg ggagcccttc aagaagagca tgaacgatct catccactcg aacggcagcg
     5821 acgcggtcgc gggccgcgcc atcatggacc agaacctggc cctcatggat caaatgaaca
     5881 cggccgtgaa cctgatgcag gctgactcgg agcagaaggt gaacgtgctc ttcgtcgtgc
     5941 agaccacggc gctcggtctc ggtctcgtgt tggtcgtagt cggtggctgg atggcgcgtt
     6001 cgaccatcgc aaagccgttg atcgagctcg cgcgcgccgc gcactcgatg agcacgggca
     6061 acctcaacgt cgagctcaac ctgagcggca cccaggaagt aaaggagctc ggggcgtcgt
     6121 tcgaccgcat gcgagcgagc atggtggcgg cgctcggcgg cggcatgggc tctacgaatc
     6181 tggacgacga cgatctctga tgggagccca aagctcagga tggtgcacgc ccgtgaagcg
     6241 ggagaatcat cgccctcgat gattgcgttc acctcgtgag cggtgcacga gtgtgcgagc
     6301 ggcgtgagtg cggagcgcag ccagtggggc tgtcgctccg cacgcatttc ggcactctga
     6361 gcatgctgaa agctccgata tccctgctcg gtcgcggcgc ggccgactct cgccaaaccc
     6421 tcatggagcg aattccagac ctggcgcttt ccgag
//
LOCUS       DASTKS010000624         5024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_547573, whole genome shotgun sequence.
ACCESSION   DASTKS010000624 DASTKS010000000
VERSION     DASTKS010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547573"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1300)
                     /locus_tag="VFQ35_12925"
     CDS             complement(92..1300)
                     /locus_tag="VFQ35_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9931593.1"
                     /translation="MTPWLRTLLASAPRRAALLSLSFAPLGITVPSRAAEPSAKAQAA
                     AHFERGVELAKEAAYAEALIEFSRAYELSPHYSTLYNIAQAYVGLGKPVQAVDTLKRY
                     LAEGGAQIGIDRRRAVEAELVKQFARTATLDIQVDGSVAANAEVSVDGEVVGRAPLPR
                     PVRVAIGVHRVSVRAENGATSEQNITVAGEEQHTLRFELTPRATSPHTTSPAASPATS
                     LGWLHVTCAAAGVRVSVDGTEVGVTPLRAPLALAAGAHRILFRASDRPEAARNVSLAP
                     ATTAELDCGFVALRGESNHSGAQKTWGYVLGAVGIGLGAATVAHFAWNYGRYNDWNDE
                     RDAYLAAPSRDKRLHANALGTSIERASAVTIVLGVGAGLALGAGTLLIVTDTSSSKGP
                     RASLSFRGSF"
     gene            complement(1380..2837)
                     /locus_tag="VFQ35_12930"
     CDS             complement(1380..2837)
                     /locus_tag="VFQ35_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9931594.1"
                     /translation="MTSTDSSRVGSVVGGKYRLVRCIGEGGMGEVYEAQHVTVGRRFA
                     LKFLQRHLAQHQDALERFRREARAAGALENEHIAGVLDFDIASDGTPFLVMEYLVGES
                     VAELLRREGPLPVTRAVGIVLQVCRGLKAAHDAGIIHRDLKPDNLFSVRRADGSELIK
                     ILDFGIAKLSSEESSPALTRSGAMMGTPFYMAPEQARGDKLLDPRVDVYALGVILYEL
                     LSAEKPHPGDSHNAILAHILTESIVPLERLRPNLPSALSALIARTLSSSRVDRPNSVE
                     ALARELAPFQGNELSPVRSQFDFRADYASAKSEVAFPPELANAKTLPTPDTHALALPA
                     TPSAPSTRPHLRRSALIAGGAALLGAFALYAARAPTPAAPNVTAPATSTRAPAPQAFT
                     ASEALTASAAPPAATPSTLVADAPRPALNPAPPMSFRTSADIAGRPGGRPHKAPRADS
                     TGVVATTSKPPESPAKTSDSQRSPRVDFDTQNPYQ"
     gene            complement(2834..4054)
                     /locus_tag="VFQ35_12935"
     CDS             complement(2834..4054)
                     /locus_tag="VFQ35_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931595.1"
                     /translation="MLELSPGRGDCVIGRDEDCAVVLSGGDVSRRHALLKSEGHRSIL
                     SDLESRNGTFVNGRAVKSTLLELNDVVRIGGWVGVVTRDHAPLGSLAPGLFGGGALRR
                     ALAAAERAAKSDLPIILEGETGTGKEAVSRALHVWSGRTGPFVAVNCAALPEALAEGE
                     LFGYRRGAFTGAEQASIGHFRAAQRGTLLLDEICELKLPLQAKLLRVLEQREVLPLGE
                     STPVRLDVRVLAAAQEPLARAVQEGRFRADLYARLDGVTLELPPLRARRAEVPYLFSR
                     LLVEHAGARPPAVDVRLIERLCLYDWPFNVRELGLLAKRLLVLHGTEPSLKVSHLPER
                     MRGDVAPPPREERKPVPNPDGPELADLLTALRECGGNVAKAAVQLGISRQRAYRMMQG
                     QVDLTALREQGDDP"
     gene            complement(4331..>5024)
                     /locus_tag="VFQ35_12940"
     CDS             complement(4331..>5024)
                     /locus_tag="VFQ35_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9931596.1"
                     /translation="SNLFSHTGTFGAIVVLTAISNVLQFQSARYLPFGLRASVILTGV
                     SLGSVFLGWGVLGERLSPTAAAWCALIVLGAALAAPGEHARHEIEPRLAPGISLSLAS
                     AALMAVVALLMKSLTERTHPLLAAWAWEFGSGLILVPALLARRAAPRFAFKQFGRVAL
                     ASSPTAIGSSASMLALGAGRLGLWGALAGTQVLFTAVLGVLWHRELIGPRRWACFLAA
                     AIGVAGLALTRA"
BASE COUNT          870 a         1597 c         1771 g          786 t
ORIGIN      
        1 gtgcacgcta gggacaggta gaagatgttc cgcgagttca tgtttatctc cgcacgcccg
       61 cgctttcgtc gcggatctgc caaccccaaa atcagaaaga gccacggaaa gacaggctcg
      121 ctcgaggacc cttggacgag ctcgtgtccg ttactatcag tagtgtgccc gcaccgagcg
      181 cgaggcctgc accgacgccg agcacgatcg tgacggcact cgcgcgttcg atcgatgtgc
      241 ccagcgcgtt cgcgtgaagt cgcttgtcgc gagagggcgc tgccaggtag gcgtcgcgct
      301 catcgttcca gtcgttgtag cggccgtagt tccaagcaaa gtgcgccacc gttgcggcgc
      361 cgaggccgat gccgaccgcg ccgagcacat agccccaggt tttttgggcg ccggagtggt
      421 tagactcgcc gcggagtgcc acgaagccac aatcgagctc tgccgtcgtg gcgggcgcga
      481 gggacacgtt gcgtgccgct tcgggacgat cgcttgctcg gaacaagatg cggtgtgcgc
      541 cggcggcgag cgcgagcggc gcgcgcagag gagtcacccc gacctcggtg ccgtcgacgc
      601 tgactcgcac gcccgctgcg gcgcacgtga cgtgcagcca gccgagcgat gtcgcgggcg
      661 aagccgcggg cgaggtcgtg tggggcgagg tcgcgcgcgg cgtgagctcg aagcgaagcg
      721 tgtgctgctc ttcgcccgcc acggtgatgt tttgctcgct cgttgcgccg ttttcggcgc
      781 gcacgctcac ccggtgcacc ccaatggcaa cgcgaaccgg ccgaggcaac ggcgcgcgcc
      841 cgacgacctc accgtcgacg ctgacctctg cgttggcagc gacggaacca tcgacttgga
      901 tgtcgagcgt agcggtgcgc gcgaactgct tgacgagctc agcctcgacc gcgcgtcgcc
      961 gatcgattcc gatttgcgcg ccgccctcgg ccaggtagcg cttcagtgtg tcgacggcct
     1021 gcacgggctt gccgagtccg acgtacgctt gggcgatatt gtacagggtc gagtagtgcg
     1081 ggctcagctc gtaagcgcgc gagaactcga tcagcgcttc ggcataggcg gcttctttgg
     1141 cgagctccac gccgcgctcg aagtgagcgg cggcttgagc cttcgcgctc ggctccgccg
     1201 cgcgactcgg aacggtgatg ccgagcggcg caaaggagag cgagagcagc gccgcgcgtc
     1261 gaggcgccga cgcaagtaag gttctgagcc agggagtcac gggcgaagct gaagccgagg
     1321 ggagggcgat gaaggcgagc acggtgtcgt gcccccgagg gagcaggata cgtgccgagt
     1381 tactgatagg ggttttgagt gtcgaaatcg acgcgcggac tgcgctgcga gtcgctggtt
     1441 tttgctggtg attcaggtgg ttttgacgtg gtggcaacga cgcccgtcga atcggctcgc
     1501 ggtgccttgt gtggacgtcc acctggacgt ccggcgatgt cggcgctcgt gcggaacgac
     1561 attggcggag ccgggttgag ggcgggacga ggagcgtcag cgacgagcgt gctcggcgtt
     1621 gcggcggggg gggccgcgct tgccgtgagg gcttcgctcg cggtgaatgc ttggggtgcg
     1681 ggggcccgtg tgctcgtcgc gggagcggtt acattggggg ccgcgggcgt cggtgcacgc
     1741 gcggcgtaga gagcaaacgc tccaaggagc gccgcaccgc cggcgatgag agcgctgcgg
     1801 cgaaggtgag ggcgggtgct gggagcactc ggggtcgcag gcaacgcgag cgcgtgcgtg
     1861 tctggtgtcg gcaaggtctt ggcattcgcg agctcgggtg ggaaagcgac ctctgacttg
     1921 gcgctcgcgt aatcggctcg gaaatcgaac tgcgaccgaa cgggcgagag ctcgttgccc
     1981 tgaaacggcg cgagttcccg cgcgagcgct tccacgctgt tgggacggtc gactcgtgag
     2041 ctggacaggg tgcgcgcaat gagcgccgaa agggcgctcg gcaggttcgg ccgcagccgc
     2101 tcgaggggca cgatggattc ggtcaggatg tgcgcgagga tggcattgtg cgagtcgccg
     2161 gggtgcggct tttctgcgct caagagctcg tagagaatga cgccgagcgc gtacacatcg
     2221 acgcgcgggt cgaggagctt gtcgccgcgg gcttgttcgg gagccatgta aaagggcgtt
     2281 cccatcatcg cgccgctgcg cgtgagcgcc ggtgacgact cctcgctcga cagcttggcg
     2341 atgccgaagt ccaagatctt gatgagctcc gagccgtccg cgcgacggac cgagaagagg
     2401 ttgtccggct tcaagtcgcg atggatgatg ccggcgtcgt gcgcggcttt gagcccacgg
     2461 cagacctgca gcacgatgcc gaccgcgcgt gtcactggca gtggaccctc gcgtcgcaag
     2521 agctcggcga cgctctctcc gacgagatac tccatgacca agaagggcgt cccgtcgctc
     2581 gcgatgtcga aatccaggac tccggcgatg tgttcgtttt cgagagcccc ggccgcgcgg
     2641 gcctcgcgac gaaagcgctc gagcgcgtcc tgatgctgcg ccaggtggcg ctgcaagaac
     2701 ttcagcgcaa agcgacgccc cacggtgacg tgctgggcct cgtagacttc gcccatcccg
     2761 ccttcaccga tgcaccgtac caggcgatac ttgccaccga ccacactgcc gacgcgactc
     2821 gagtcggtcg aggtcaaggg tcgtctccct gctcgcgcag tgccgtgagg tcgacttgcc
     2881 cttgcatcat gcggtaggcg cgctgcctcg agatcccgag ctgcaccgcg gccttggcga
     2941 cgttgccgcc acactcgcgt agggccgtga gcaagtcagc gagctcgggg ccgtccgggt
     3001 tgggcacagg tttccgctct tcgcgcggcg gcggcgccac atcgcctcgc atccgctcgg
     3061 gcaaatgcga aaccttcaac gacggctccg taccgtgcag caccagcagg cgcttcgcga
     3121 gcaacccgag ctcacgcacg ttgaagggcc agtcataaag gcacaggcgc tctatcagac
     3181 gcacgtcgac ggcgggtggg cgcgcgcccg cgtgctcgac gagcaggcgt gaaaacaggt
     3241 acgggacctc cgcgcggcga gcgcgcagcg gcggaagctc gagtgtgaca ccgtcgagac
     3301 gtgcgtaaag atccgctcga aagcgacctt cttggactgc tcgcgcgagc ggctcttggg
     3361 cggccgcgag gactcgaaca tcgaggcgca ccggcgtcga ttcgccgagc ggcaagacct
     3421 cacgttgctc gagcacgcgc aggagcttcg cttgcagcgg cagcttgagc tcacagattt
     3481 cgtcgagcag cagcgtgccg cgttgggccg cacgaaaatg cccaatgcta gcttgttcgg
     3541 ctcccgtaaa ggcgccgcgt cgatagccaa aaagctctcc ttccgcgagc gcctcgggca
     3601 gcgccgcgca gttcacggcg acgaagggcc ccgtgcggcc gctccaaaca tgcagtgcgc
     3661 gggacaccgc ctccttgcca gtccccgtct ccccttcgag aattatgggc aggtcgctct
     3721 tcgcggcccg ctccgcggct gcgagcgcgc gccgcagcgc tccgcctccg aacagccccg
     3781 gcgccaaact gccgagcggc gcgtgatcgc gcgtcacgac ccccacccag cccccgattc
     3841 gcacgacatc gttcagctcg agcaacgtgc tcttcacggc tcgcccgttc acgaacgtgc
     3901 cgttgcggct ctcgagatcc gagagaatcg agcggtgtcc ttcgctcttc aacagcgcgt
     3961 ggcggcgaga cacgtcacct ccgctcaaca ccaccgcgca atcttcatcg cgtccgatca
     4021 cgcaatcgcc gcgcccgggg gagagctcca gcacggggcc gtccgtgcgg gggaacaacc
     4081 acgccaatac gcacggcccc tgaccctccg aaccaaatgt cgaattgaac gagggagcgg
     4141 tggtatccgg caatcgacgc ggcactattc tttctccagc ccacccggag gtgcccgtat
     4201 cataacgccg gaccgtccaa gagctcgcga cctggcgtcg tccctggcaa aagccgcata
     4261 aatcgccggt ctgaacgcgc ggccagcacc ccttgggcgc tcgagaaaac tagatgtcga
     4321 agccttggat ctacgcccgc gtgagcgcga gcccggccac gccaatcgct gcggcgagga
     4381 agcaggccca gcggcgcgga ccgatgagtt ctcgatgcca gagtacaccg agcacggcgg
     4441 tgaagagcac ttgagtgccg gcgagtgcgc cccagagccc gaggcggcct gccccgaggg
     4501 cgagcatgga ggcgctcgag ccgatggcgg tgggtgagga ggcgagcgct acgcggccga
     4561 attgtttgaa tgcgaaccgg ggcgcggcgc ggcgggcgag caacgcgggg acgaggatga
     4621 gccccgagcc aaactcccaa gcccaagctg cgagtagggg gtgagtgcgc tcggtgaggg
     4681 atttcatgag gagagcgacc acggccatca gagcggcgct ggcgagggag agcgagatcc
     4741 cgggcgcgag gcgcggttca atttcgtgcc gcgcgtgctc gccaggtgcc gcgagcgcag
     4801 caccgaggac gatcagcgca caccacgcgg cggcggtagg cgacaatctc tctccgagta
     4861 cgccccagcc gaggaagacg ctgccgaggg aaacgccggt gaggatgacc gacgcgcgca
     4921 agccgaacgg gaggtagcgc gcggattgga actgcaggac attcgagata gcggtgagca
     4981 cgacgatcgc gccgaaggta cccgtgtgcg aaaaaaggtt cgac
//
LOCUS       DASTKS010000625         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_547708, whole genome shotgun sequence.
ACCESSION   DASTKS010000625 DASTKS010000000
VERSION     DASTKS010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547708"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFQ35_12945"
     CDS             <1..939
                     /locus_tag="VFQ35_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931597.1"
                     /translation="SVCERIKARMPLLPESMKGRLDQIVVALEELQARATEMIPGAVK
                     ELWQRLEAIQKSLYEGQWHAASVGEKTATRTLERKLVKSESAGAAARWEMSTVDFPGL
                     KSIADLPEAKGWPAQRSTTRLTPAELAAACHTVPTPVWLPPGTELVRVISESKRAPGV
                     WWMFKKDLPKNGPDWRNDFAVLEYWSSNGLKVEVTVPEGGAWIWMSQVASQLQTSTEL
                     PGAGQYLRGGATQLFLDLDFAAHRDLKAAVLAAAPTPTNWDAAKLVNVGTPEAPAVTT
                     TAVEGVMPKPGVIEQLSRSSRVAASASSNQSQDAHP"
     gene            936..1838
                     /locus_tag="VFQ35_12950"
     CDS             936..1838
                     /locus_tag="VFQ35_12950"
                     /inference="COORDINATES: protein motif:HMM:NF026922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm72 family immunity protein"
                     /protein_id="HET9931598.1"
                     /translation="MKNWIFGNPVFSTTQSLEGNELPVTDAERARAFWRLRRYTSFAY
                     IERQAEHLNQLIEGFKSEARRAADPYAMRDFTQMLAVLYRHDSHYQKGLALLRPGDVG
                     GFDFILEGAKVASYFESRMFYEHEDLRWFAFGSEEARPLVGLLAWGRRTYTLARNTLR
                     TLRAEWTFELLRDPRPSRDPCRLPERCAALPAPQPGAARCSPGQPVPTTGVWLPTDRR
                     SGCPNYLVSRWIAPEAMATVASSENAAIESGYPEYTYERRASRWELCWEDVRLSEPET
                     RGGSEFLGDDVAFPDEPLEIMPHS"
     gene            1909..>4341
                     /locus_tag="VFQ35_12955"
     CDS             1909..>4341
                     /locus_tag="VFQ35_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HET9931599.1"
                     /translation="MNVGKEQPSLTQTLERARRELLDLTARNRLLHVPRDRAKSGRID
                     IVRERADDIYRLLVNDGRSMSFLVSKEPRPEDAEEDEAAYTDDALQTSLDEKVLEKRL
                     LRMYYDARTFEDEQGSNILFLAIGFLRWHEPDEAKTVREAPLLLVPVRLERRRAGGNF
                     TLSYDETELNQNLSLAEKLKGFGIELPELPEPDELIPTKFFAAVRTAVAAQAGWEVDE
                     NGIVLWFFSFAKFLMFRDLQPQAWPAPGLDGNPLIRALLRDGFANSPPAVSDEANLDR
                     ALRPRDLVHIVDADSSQTVATEEVNRGRSLVIQGPPGTGKSQTIANIIAAAILRDRKV
                     LFVAEKTAALKVVKQRLEAAGLGPACLELHSHKANKVAVLEELRRTLELELSADANID
                     ESAESLRRERERLNEYVEALHSPLTAARVTAFEAMSQLLALGQDGHVPVHVEHAGAES
                     WTRSDLEERRRTLEEVALTLEYIGVPAAHPCRGVHAATLLPNDKSRLLVELARILQEL
                     TLLETEAAELAALFGATGVQSLGQATLIAQQLRSIAQAPNLPLSSVAAPVWDNAPEEP
                     PRLVAAGHRLTALAPILTRAFVDTAWNADVSQIRTAVAAHGSSLLRWVSGDFRDAEAA
                     LNGLLREQPPPPSSECVALLDQLIEAQHIRQLLATSSSGPEAFGALWRGEASSFEALA
                     ALGQWVDAVRTQDPARSIRLAAARLGSPQAAGERLRGFEARLSQLYTTLHVATSTLAL
                     DLREAFGVATLSEVPLAALRERFGQWLEAPMILDRWCAYAAAVSALPRLGLDALVAPI
                     AKGSISPAALVRD"
BASE COUNT          809 a         1439 c         1379 g          714 t
ORIGIN      
        1 tcggtctgtg aacgaatcaa ggcgcgcatg ccgctcttgc cggagtcgat gaaggggcgc
       61 ctcgatcaaa tcgtcgtcgc gctggaggag ctccaagcgc gggccaccga aatgatcccc
      121 ggggcggtga aagagctctg gcaacggctc gaagccatcc aaaagtcctt gtacgaaggg
      181 caatggcacg ccgcttccgt gggcgagaag accgcgacgc gaaccctgga acgcaagctc
      241 gtgaaatccg agtccgcggg tgcggccgcg aggtgggaga tgagcacggt cgactttccc
      301 ggtctcaaat ccatagcgga cctccccgag gccaagggct ggcccgcaca gcgaagcacg
      361 acgcgcttga cccccgccga actcgcggcg gcctgccata ccgtcccgac ccccgtgtgg
      421 ctgccacctg ggacggagct cgtccgcgtc atcagcgagt ccaagcgcgc gcccggggtg
      481 tggtggatgt tcaagaagga ccttccgaag aacggccccg attggcgaaa cgatttcgcg
      541 gtgctcgagt actggagctc caacggcctc aaagtcgagg ttacggtacc ggaaggcggg
      601 gcttggattt ggatgtcgca ggtcgcgagc cagctccaaa cgagcaccga actgccgggc
      661 gccgggcagt acctgcgcgg tggcgccacc cagctcttcc tcgacttgga tttcgcggct
      721 catagagatc tcaaggcggc ggtgctggcg gcggcaccaa cgcccactaa ttgggatgcc
      781 gcgaaactcg tcaacgtggg cacgccggaa gcgcctgccg ttacgaccac cgcggtcgaa
      841 ggcgtcatgc ccaaaccagg cgtcatcgaa caactgtcga gaagcagccg agtcgccgcg
      901 agcgcttcgt cgaatcaatc ccaggacgcg cacccatgaa gaactggatc ttcggaaacc
      961 ccgtattttc gacgacgcaa agcctcgaag ggaacgagct acccgtaacc gacgccgagc
     1021 gtgcgcgagc cttctggcgg ctccgccgct acacgtcctt cgcgtacatc gagcgacagg
     1081 cggagcattt gaatcagctg atcgagggct tcaaatcgga ggcccggcgg gccgcggacc
     1141 cgtacgcaat gcgagacttc acgcaaatgt tggcggtgct ctaccgccac gactcgcact
     1201 accaaaaggg cctcgcgctg ttgcgtccag gtgacgtggg cggcttcgac ttcatcctcg
     1261 aaggcgcgaa ggtcgcgagc tacttcgaat cgcgcatgtt ctacgagcac gaggacctgc
     1321 ggtggttcgc gttcggcagc gaagaagctc gcccgctggt cgggctcttg gcatgggggc
     1381 gtcgcacgta cacgctggca aggaatacgc ttcgaacctt gcgtgccgag tggacgttcg
     1441 agcttctgcg cgatccgcgc ccttctcgag acccttgccg attgccggag cgctgtgcgg
     1501 ccctccccgc tccgcaacca ggcgccgcgc gctgctcgcc ggggcaaccg gttccgacga
     1561 caggcgtgtg gctgccgacc gatcgccgat ccggttgccc gaactacctc gtttcgaggt
     1621 ggatcgcgcc cgaggcaatg gccaccgtgg catcgtccga aaacgcggcc atcgagagcg
     1681 gctaccccga atacacctac gagcggcgcg cgagccggtg ggaactctgt tgggaagacg
     1741 tccgtctttc cgaacccgag acccgcggcg gctccgagtt cctgggtgac gacgtcgcct
     1801 tcccagacga accactggag atcatgcccc attcgtagac gcgcccaccg acgcgcgcgt
     1861 gaactttggc gcacgacacg gagttctgta ggctcccggc gcttgccaat gaacgtcggg
     1921 aaagagcaac cgtcactcac ccaaactctc gaacgagcgc gccgtgagct gctcgatctc
     1981 accgcgcgaa accgcttgct tcacgtcccc cgcgatcgcg cgaagtccgg acgtatcgac
     2041 atcgtacgcg aacgggcaga cgacatctac cgcttgctcg tcaacgacgg ccgctcgatg
     2101 tcgttcctcg tgtccaagga gccgaggccc gaagacgcgg aagaagacga agcggcgtac
     2161 accgacgacg cgctgcaaac atcgctcgac gagaaggtgc tggaaaaacg cctcttgcgc
     2221 atgtactacg acgcgcgcac gttcgaagac gaacagggct cgaacatcct gtttttggcc
     2281 atcgggttct tgcgctggca cgaaccggac gaagcgaaaa cggtgcgcga ggcgccgctt
     2341 ctgctcgtcc ccgtccgttt ggagcggcga cgcgcaggcg gtaacttcac gctgagctac
     2401 gacgaaacgg agctgaacca gaacctttcg ctggccgaaa agctcaaggg gttcggcatc
     2461 gagctcccgg aattgcccga acccgacgag ctgattccta cgaagttttt cgccgcggtc
     2521 aggacggccg tcgcagcgca ggcgggctgg gaagtggacg agaacgggat cgttctttgg
     2581 ttcttctcgt tcgcgaagtt tctgatgttc cgggaccttc agccgcaagc gtggccggcg
     2641 cctggcctcg acggcaatcc gttgattcgg gcattgctgc gcgacggttt tgcgaattct
     2701 ccgccggcgg tctcggacga agcgaatctc gatcgggccc tccgtccgcg tgacttggtg
     2761 cacatcgtcg acgccgactc atcgcagacg gtcgcaacgg aagaggtcaa ccgcggccgg
     2821 tctctagtca ttcaaggccc gccgggaacc ggaaagtcgc aaaccatcgc gaacatcatc
     2881 gccgctgcga ttctgcgaga tcgcaaagtc ttgttcgtcg cagagaagac cgccgcgctc
     2941 aaggtggtaa agcagcgact cgaggccgcg ggcctcggac ctgcctgcct cgagctgcac
     3001 agccacaaag ccaacaaagt cgccgtgctc gaggagttgc gtcgcacgct cgagctcgag
     3061 ctttcggccg atgcgaacat cgacgaaagc gccgaatcgc tgcggcgcga acgtgaacga
     3121 ctgaacgaat acgtcgaagc gttgcactct cccctcaccg ccgcgcgcgt gaccgccttc
     3181 gaggcaatga gccagcttct cgcgctcggc caggacggcc acgtacccgt gcacgttgag
     3241 cacgcgggcg ctgaaagttg gacgcgcagt gacttggaag agcgacgtcg cacgctcgag
     3301 gaggtcgcgc tcacgctcga gtacatcggc gttcctgccg cgcacccgtg ccgaggcgtg
     3361 cacgccgcca ctctgctccc aaacgacaag agccgtctcc tcgtcgagct cgcgcgcatc
     3421 ctccaggaac tcacgctgct cgaaacagag gccgccgagc ttgcggctct gttcggggcg
     3481 acgggagtcc aaagcctcgg gcaggcgacg ctcatcgccc agcaactccg ctcgatagcc
     3541 caggcgccga acctacctct ctccagcgtc gctgcgcccg tctgggacaa cgctccagag
     3601 gaaccgccac ggctcgtcgc ggccggccac cggctcacgg cgctagcgcc gatactgact
     3661 cgagcgttcg tggacacagc ctggaacgcc gatgtttcgc agatccgtac cgcggtcgca
     3721 gctcatggaa gctcgctcct gcgctgggtc agcggggact ttcgcgacgc cgaagcggcc
     3781 ctcaacgggc tgctcaggga gcaaccacct cctccgtcca gtgagtgcgt ggcgctgttg
     3841 gaccagctca tcgaggcgca acacattcgc caactgctcg cgacgagttc gtccgggcca
     3901 gaggcgtttg gcgcgttgtg gcggggcgaa gcatcgagct tcgaggcgct ggcggctctc
     3961 gggcaatggg tggacgccgt tcggacgcaa gatcccgcgc ggagcatccg cttagctgca
     4021 gcgcgcctcg gctcgcctca agcagccggg gagcggctgc gcggattcga agcacggcta
     4081 tcacagctct acacgaccct gcacgtcgcg acgagcacgc tcgcgctgga tctcagggag
     4141 gctttcggcg tcgcgaccct gagcgaagtt cccctcgcgg cgctccgcga acgcttcggt
     4201 caatggctgg aagctccgat gatcctcgac cggtggtgcg cttacgcggc ggcggtgtcc
     4261 gcgttgcccc gactgggcct cgacgcgctc gttgcgccga tcgctaaggg ttcgatttcg
     4321 cccgccgcgc tcgtacgcga c
//
LOCUS       DASTKS010000626         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_324081, whole genome shotgun sequence.
ACCESSION   DASTKS010000626 DASTKS010000000
VERSION     DASTKS010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324081"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1522
                     /locus_tag="VFQ35_12960"
     CDS             86..1522
                     /locus_tag="VFQ35_12960"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931600.1"
                     /translation="MPTAATNTRTAKDPDTSDDAPVIPLGTHLFLVLEAERPRAGGAR
                     YSLAETNEVVIGRGPDRTVRRDRSGSGTRLRISLPSSRVSSLHARLRNHRGHWTIEDL
                     GSTNGTFVNGIRVTEAVLNDRDSLEIGQVTLIFRDALGTPGGPARELELGPSTDGLQV
                     RSLLPLEEERMRVLSAMARTPLPVLLLGESGTGKEVLARAVHAQSGRLGSLVGFNCGG
                     LSSGLFESQLFGHTRGAFSGATRDELGLVRAAEGGTLFLDEIGDMPPAAQVALLRVLQ
                     EGEVLPLGTARPCRVDVRVIAATHRPLHQLSASGTFRADLLARLHGHLHTLPALRHRR
                     EDLGVLLADLLTELDPAQRIRRLSADAARSLLAHSWPLNVRQLRHALTRAAVLVEDGV
                     LRRRDLDPEPFVLDSEPEASGAAPRPALSASDEELRKRLLALLSAERGNVAEVARALG
                     KARMQVQRWMKRFGIDPANYRDSSRGDP"
     gene            complement(1558..2430)
                     /locus_tag="VFQ35_12965"
     CDS             complement(1558..2430)
                     /locus_tag="VFQ35_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931601.1"
                     /translation="MADLVTRGLERRRERRDTEALALFRDAYSISATPMILAQIALAE
                     QALGQWLLAEQDLTAALATHDGWVEANRDALDRALVVIQSHLSWLAVTTNVESAEVWL
                     DDRKLGVVRAAPWRVIQGPHELSLRLADGRSSTRSLELSAGAREHFHVDFSPLLPSIA
                     SGPHARAPRVRQSAKTFDAANATPETNTRKTLAFASAALSVSAFAGAVTASLLRLEYA
                     HHYNSDACAPDRSQKCAAYRDVAERLGTVAVVGYAVAGVGALGSVALFTAPYWLPGPD
                     PKASQAGLCVWGTF"
     gene            complement(2516..3898)
                     /locus_tag="VFQ35_12970"
     CDS             complement(2516..3898)
                     /locus_tag="VFQ35_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9931602.1"
                     /translation="MTAPDHSLGVRAGPDAVGRYQLLEPLGRGGMGTVYRARQVQLGK
                     LVAVKLLDPVIASGAAGVTRFMREARAAAQIHHPNVVEVFDVGVDEGAVFLAMELLQG
                     HDLAALLRSRRLELHEVVELFAPVLAGVRAAHEAGVVHRDLKPSNIFLARRGRTRREP
                     VVLDFGVSRVLLDDELGLTPVTSPDAMIGTAAYIAPEQAKQARLADARSDQFSLGVIL
                     YECATGSRPFSGQNTFELLHSIATATVVPPSERNPSLPRAFDELVLLVLSRAPQERFE
                     DLDAMARALMTFAAPRTWMAWGRELVADWADNDASPTAPPSEPHRSPKPLAASPAPAL
                     EATPRWRGLTVAFGLGALSSAAVAMLSSRPLAPQAAEVPLPRAVAATSLSSPPRTELA
                     EDAARPLATASTEGSSRSRANSARSVALRPPPPRIAPSARPHGEAHPSATARAPLPVE
                     LGQNDAPIIK"
     gene            complement(4073..>5105)
                     /locus_tag="VFQ35_12975"
     CDS             complement(4073..>5105)
                     /locus_tag="VFQ35_12975"
                     /inference="COORDINATES: protein motif:HMM:NF016345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HET9931603.1"
                     /translation="TINASAIYTRVSAPPRSAEQNLDTVAGLMAGKKFLETWYPKIAN
                     IIAYPAYAPATSITINGASQIIKEGENRGCLTDPNNKNTSAAGWVAGGTTLNICETSA
                     HDMGLYVHESTHIIQKNYLDNPKLSGLPESVASWAGNLSIGRQNTKPSQYMSFYDDYQ
                     YGAYFYDFVAESHPRFLEQLNQATFLGQYDDTWLTRNVQRTLGQLWGDMAGTGFTSPG
                     ALRNGTGQYIYPKDKGDESDHTTVGLRIRLNPPDNPARFFRGQIVEGKGLLRWEKDYC
                     FGENTGLVVLQTCDETADRWSYQDGAFVNARTNRCMQALAASTADMTPIGTAVCNGSA
                     AQHWEPLPL"
BASE COUNT          879 a         1640 c         1693 g          893 t
ORIGIN      
        1 tcgacgcggc cgtagcgacg gcggtgcaac gcaaacaatc ggaaggcggc gcgcggaagc
       61 ctaggttacc ttcgaggctg cgctgatgcc caccgctgcc accaacactc gcacggccaa
      121 ggatccggat acttcagacg acgcgcccgt catcccgctc ggcacacact tgttcttggt
      181 tctggaggcc gagcggccgc gcgcaggggg agcgcgttac agcctggcag agacgaacga
      241 ggtggtcatc gggcggggtc cagatcgcac ggtgcgccgc gatcgtagcg ggagcgggac
      301 gcggcttcgg atcagcttgc cgtcgtcgcg ggtttccagc cttcacgcgc ggctccgcaa
      361 ccatcgcggc cactggacga ttgaagatct cggatcgacc aacgggacct tcgtcaacgg
      421 cattcgcgtg accgaggcgg tgctcaatga ccgtgactcg ttggaaattg ggcaggttac
      481 actgattttc cgcgacgcgc tcgggacgcc cggagggccc gcacgggagc tcgagctcgg
      541 accgagtacc gacgggctgc aggtacgttc gctgctgccg ctcgaagaag agcgaatgcg
      601 ggtgctgagc gcgatggcgc gcaccccgct ccccgtcttg ctgctcggtg aatccggcac
      661 cgggaaggaa gtgctggcgc gcgcggtgca tgcgcagagc gggcgcctcg gctcgctcgt
      721 ggggttcaac tgcggcggcc tgagttcggg cctcttcgag tcacaactgt tcggacatac
      781 gcgcggcgca ttttcgggtg caacgcgtga cgagctcggg ctcgtccgcg ccgccgaagg
      841 tgggacgcta ttcttggacg agattggcga catgccgccc gcggcgcagg tcgcgctgct
      901 gcgggtcctg caagaaggcg aggtcctccc tctcggcacg gctcgtccgt gtcgagtcga
      961 cgtgcgcgtg atcgcggcga cccaccgccc gctccatcag ctgagcgcat cgggtacttt
     1021 ccgcgctgat ctcttggcgc gtttgcacgg ccatttgcat acgttgccgg cgttacgtca
     1081 tcggcgggaa gatctcggcg tcttgctcgc ggatctgctg acggagctcg acccagcgca
     1141 gcgcattcgt cgcctttccg cggacgccgc tcgttcgcta ctggcgcatt cctggccgct
     1201 gaacgtgcgt cagctccggc acgcgttgac gcgcgcggcg gtgttggtcg aagacggcgt
     1261 actgcgccgt cgagacctcg accccgagcc tttcgtgctc gactccgagc ccgaagcgtc
     1321 cggagccgcg ccacgacctg cgctttccgc gagcgacgag gagctccgca aacgactcct
     1381 cgcgctgctc agcgcggagc ggggcaacgt cgccgaagtc gcacgtgccc tcggcaaagc
     1441 gcgcatgcaa gtgcagcgct ggatgaagcg cttcggtatc gatcccgcga attaccgcga
     1501 ctcgtcgcgc ggtgacccct agctcatctc gagcaaccga tgggcattcg cgggccatta
     1561 gaacgtgccc catacgcaga gccccgcctg gctcgccttg ggatcgggac ctggaagcca
     1621 atacggcgcc gtgaacaacg ccacggaacc caaggcacct actcccgcga ccgcgtaccc
     1681 gacgacggca accgtcccga gtctctcggc cacgtcgcga taggcggcgc acttctggct
     1741 gcgatccgga gcgcaagcgt cgctattgta gtgatgcgca tactcgagcc tcagaagcga
     1801 ggccgtcacc gcgccggcga atgcgctcac ggaaagagcg gccgaagcaa aggcgagagt
     1861 ctttcgcgtg ttcgtctcgg gcgtggcgtt cgccgcgtcg aaggtttttg ccgattggcg
     1921 cacacgcggc gcacgcgcat gcggcccgga cgcaatggag ggtagaagag gggaaaagtc
     1981 gacgtggaag tgctctcgcg ctcctgccga gagctcgagg gaacgcgtgg atgaacgacc
     2041 gtccgcgaga cgcaacgaaa gttcgtgcgg gccctgtatc actcgccaag gggcggcgcg
     2101 aacgacaccc aacttgcggt cgtcgagcca gacctccgct gactcgacgt tggtggtgac
     2161 cgctaaccag ctcaggtgcg actgaatgac gaccaatgct ctatcgagcg cgtctcggtt
     2221 tgcttcgacc cagccgtcgt gagtggcgag cgcggcggtc aggtcttgtt cggccaaaag
     2281 ccactggccg agggcttgct ccgcgagcgc gatctgagcc aaaatcatcg gcgtcgcgga
     2341 gatcgagtaa gcgtcacgga acagcgccaa tgcctccgtg tcgcgccgct cgcgccgccg
     2401 ttcgaggcct ctcgtgacga ggtctgccac ggactcttcg gcgagagccg ggcgacaaat
     2461 ggtgcccaag cacgacatga gcacgagaca agacgcgaac gtcgagaggc gtcgcttact
     2521 tgatgatcgg tgcatcgttc tgtccgagtt cgaccgggag tggtgcgcgc gcagttgcgc
     2581 tcgggtgcgc ttcgccgtgc ggccgcgcgc tcggggcaat gcgcggtggc ggcgggcgta
     2641 gcgccacgct ccgcgccgag ttcgcccggc tccgagacga accctctgtg gacgcggtcg
     2701 caagcgggcg agccgcgtct tctgccagct cggtgcgagg cggcgaggag agactcgtcg
     2761 ccgccaccgc gcgcggcagc ggcacctcgg cagcttgggg cgcgagaggc cgactcgaaa
     2821 gcatcgcaac ggccgcgctc gacagcgccc ccaacccgaa tgcaaccgtc agcccgcgcc
     2881 agcgaggagt ggcttcgagc gccggggccg gcgaagccgc gagcggtttc ggcgaacggt
     2941 gcggttcgga tggtggcgcg gtcggcgaag catcgttgtc agcccaatcc gcgacgagct
     3001 cacgccccca ggccatccac gtacgtggcg ctgcgaacgt catgagggct cgcgccatcg
     3061 cgtcgagatc ctcgaaacgt tcttggggag cgcgcgacaa cacgagcaac acgagctcgt
     3121 cgaacgcgcg cggcagcgaa gggttccttt cgctcggcgg aacgacggtc gcggtcgcta
     3181 ttgaatgcaa gagctcgaaa gtgttctgtc cggaaaacgg gcgcgagccg gtagcgcatt
     3241 cgtacaagat gacgccaaga gagaattggt cgctccgcgc gtctgccaac cgcgcttgct
     3301 tggcttgttc aggcgcgatg tacgcggcgg tgccgatcat cgcgtccggc gaagtcaccg
     3361 gggtgagccc tagctcgtca tcgagcagca cgcgcgacac gccgaagtcc aagaccaccg
     3421 gctcgcgtcg agtgcgcccg cgacgtgcca gaaatatgtt gctcggcttt agatcgcggt
     3481 ggacgacgcc ggcctcgtga gccgcgcgca ctcccgccaa gacaggcgcg aagagctcga
     3541 cgacctcgtg gagctccaag cgtcgcgagc gaagcaacgc ggccaggtcg tgcccctgca
     3601 gcaactccat ggccaaaaag accgctccct catcgacccc gacatcgaag acttcgacga
     3661 cgttgggatg gtgaatctgc gcggccgcgc gcgcttcgcg catgaaacgc gtcactcccg
     3721 ccgccccgct cgcgatgacc ggatcgagga gtttgaccgc gaccagcttc cccaattgca
     3781 cttggcgcgc gcgataaacg gtgcccatgc cgccgcgacc gagcggctcg agcaactggt
     3841 agcggccgac ggcatccggg cccgctcgaa ctcccagaga atggtccggc gcggtcatat
     3901 agttgcccgc gcagattaca ctaggaagcg ccgcctccga accccactcg cccggtccga
     3961 tcccgtttcg cagctcgact gcaggccggc tcaacactcc tgaatgttgg gccggccgca
     4021 gttcactgaa ggcgcgcgga gtcgaacctc tcgaacacct ccgggattac tctcagagcg
     4081 gaagcggctc ccagtgctgt gccgctgagc cgttgcagac cgccgtgccg atcggcgtca
     4141 tgtccgcggt ggacgcggcg agcgcttgca tgcatcgatt ggtcctcgcg ttgacgaagg
     4201 cgccatcttg gtagctccac cgatcggcgg tctcgtcgca cgtctgcaac acgactaacc
     4261 cggtgttctc gccaaagcag tagtccttct cccagcgcag caggcccttg ccttccacga
     4321 tttgtcctcg aaagaagcgt gccgggttat ctggcggatt gagacgaatc cgcagcccga
     4381 ccgtcgtgtg gtccgactcg tcgcccttgt ccttgggata gatgtactgc ccggtgccgt
     4441 ttcggagcgc gcctggtgag gtgaaacccg taccggccat gtcgccccag agttgtccca
     4501 acgtgcgttg gacgtttctg gttagccacg tgtcatcgta ctgtccgagg aacgtggctt
     4561 gattgagctg ctccaggaaa cggggatggc tctccgcaac gaagtcgtag aaatacgcgc
     4621 catactggta gtcgtcatag aagctcatgt actggctggg cttggtattc tgacgaccga
     4681 tcgagagatt gcctgcccag gaggcgacgc tctccgggag tccggacagt ttcgggttgt
     4741 ccaagtagtt cttttgaatg atgtgggtcg actcatggac gtagaggccc atgtcgtgag
     4801 ctgacgtttc gcagatgttc agcgttgtcc cgccagctac ccaacccgcg gcggacgtgt
     4861 tcttgttgtt gggatcggtc aggcagccac ggttctcacc ctccttgatg atctggctag
     4921 cgccgttgat ggtgatcgag gtggcgggcg cgtacgctgg gtaggcgatg atgtttgcga
     4981 tcttgggata ccaagtctcc aagaacttct taccggccat taagcccgcg acggtgtcga
     5041 gattttgctc ggcgctcctt ggcggagcag acacgcgggt gtagatcgcg ctcgcgttga
     5101 tggtg
//
LOCUS       DASTKS010000627         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_769846, whole genome shotgun sequence.
ACCESSION   DASTKS010000627 DASTKS010000000
VERSION     DASTKS010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769846"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            724..2136
                     /locus_tag="VFQ35_12980"
     CDS             724..2136
                     /locus_tag="VFQ35_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9931604.1"
                     /translation="MIGLQGDIPVPQAETVRHSAKGRWIDDWDPENEEFWERVGKRVA
                     RRNLVWSILAEHIGFSVWLSWSVVAARLPAAGFPYTTDQLFALVALPGLVGALVRFPY
                     TFAVPKFGGRNWTLVSALLLLIPTLSLIVLVQRPSTPLWCMQLAAATAGLGGGNFSSS
                     MTNISFFFPDRQKGWALGLNAAGGNIGVSSVQLVVPLVVGYGLLPGSTQKVHLENAGL
                     IWLLPILVATIGAWFYMDNLSQARSSFKDQVVVGRRAQTWIMSFLYVGTFGSFVGFSA
                     AFPLLLKTQFPSVTANIAFLGALVGSLSRPFGGWLADKFGGARITFINFFAMALGSVG
                     VLAALGRHDIVLFLVAFLALFVTTGIGNGSTFRMIPVIFRAVHGVDQQTGEARAAAAA
                     RARRESAAVIGIAAAIGALGGYFIPRSFAASIKASGGPMLAVKYFLAFYLLCCISTWW
                     FYMRRRFLTKLLPSLAEAKA"
     gene            2133..3839
                     /locus_tag="VFQ35_12985"
     CDS             2133..3839
                     /locus_tag="VFQ35_12985"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9931605.1"
                     /translation="MTALRNRVLSPQPKGAAPGDYESGVVVSARRREPGEPRRLPLVN
                     VAAALAAQHELTAVERFSNLHDENAVPGRQHYRALLPLTPPRSGEQYAFEVDLDACTG
                     CKACVAACHTMNGLDGGETFRAVGLLVGGTVDEPRLQTVTSSCHHCLEPACMSGCPVG
                     AYEKDALTGIVRHLDDQCFGCQYCTLMCPYDAPKYNEAKGIVRKCDMCSGRLAHGEAP
                     ACVEACPNEAIAIRVVEKAVVAAAADARHFVRGAADRDNTLPTTSFKTARATPDNMLP
                     VDYYRVAVSPAHAPLVAMLTLSQWAVGLTWAEAVFADSRGSVVLASVEVGVLGVALLA
                     SLLHLGRPWLAPRAVLGVKTSWLSREVLAFGILMKLALLKLGLTLLPSIPSFHGRELV
                     ATLGGALVQVVPALGAFAVFCSVMVYVATRRSPWSFAKTSLRFFGTTALLGAASFLAL
                     FNGQLDGASRETLAHAVAAIGLLKLGFDTWLLLEARSRRFSGDKRMALAMLGPLGRVG
                     WSRLSCLVVGAAVPLTLLASNTVSAAGLWLGTALLLGAELAERFLFFSAAPSLRMPGN
                     LR"
     gene            3836..>4855
                     /locus_tag="VFQ35_12990"
     CDS             3836..>4855
                     /locus_tag="VFQ35_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9931606.1"
                     /translation="MKSVAEGLQQLKDAIIRRDGPLTRELVLKPSRFGLGRLPEKAQP
                     DAVLRTVCGFCSTGCSLDVLLQKQKLLGLVPSRGYPVNQGAACPKGWEALAPLTGSGR
                     ATTPLVRRDGKLVPVSWETALSEMVQRFKAIQEAHGPESLAWLGTGQMPTEELAFLGA
                     LAKFGMGIIHGDGNTRQCMATAAVAYKQSFGFDAPPLAYADFEESDVIVLVGSNLCIA
                     HPILWQRVCQNRRSPKIVVIDPRATETAMAATHHFAVKPKGDLALLYAVANELIARGH
                     IDRAFIDAHTTGFDDFAREVARFTPESVSAESGIEPERIRQLARLIHESERVSLWWTM
                     GVNQSH"
BASE COUNT          760 a         1547 c         1593 g          955 t
ORIGIN      
        1 ggactaccgg gcgaggctct gagcgcagcg ctcgagctcc gaacggctcg agcgctgcgt
       61 tcacttcgtc gtcaccccgg agcgttcact ctagaggcaa aggtcccgcg aatggacaag
      121 tcatactgcg cgggccgatc ctcttgctgc ggcgtgcggg ctcgtcgttg cgctcctcga
      181 cctgcttcag caggcctggg tgctctctcc tcacgcgccc gcctcgctgc ggtctcggcc
      241 ccctcgcgac tgacttgttc attcgcggcc ctagacgacc acgttggcgg tgtcgcaaag
      301 cgccacccgg cgaagtgaaa gttgcacgtg gttgtgcggg acgcggcgcg aggcgatctt
      361 cgtgtcgttc ccggtcatga aaactttcca tcgcgcgata ttgaacttgg gtcgcgccca
      421 cgcaaacgag ttggggagtc actcgatgca gttgctgaat cgcgcgtcga gaactcaatc
      481 acccgttagt cggaactcgc cacgacgttg acgactaaag ctctcgcgct tggtgtcgtt
      541 ccataatgcg cggagctggc tcggcggagg gctgccgaac cgacgcgcgt gaaagcgaaa
      601 gagttcagtg agagagggtg aacttattcc taaggctgat ttgccgaagc cgcgtaaggc
      661 tccgcgactt gccgaccaat acccgcgcgg acccagaacg tcccagaacc aaaaggtgga
      721 gacatgatcg gactacaagg agacatccct gtaccccaag cggaaaccgt acggcactcg
      781 gcaaaggggc ggtggatcga tgattgggat cccgaaaacg aggagttctg ggaaagggtg
      841 gggaagcgag tagcgcgccg gaacttggtc tggtccatcc tcgcggaaca catcggtttc
      901 tcggtctggc tctcgtggag cgtcgtcgct gcgcgcctac cggcggcagg cttcccctac
      961 acgaccgatc agctgttcgc cctcgtcgcg ctgccaggcc tcgtcggggc cttggtgcga
     1021 ttcccataca cgtttgcggt tcccaagttc ggcggaagga attggacgct cgttagcgca
     1081 ctcttgctgc tgattccgac tctgtcgttg atcgtgctcg tgcagcgccc gagtacacct
     1141 ctctggtgca tgcagctggc ggcggcaacc gccgggttgg gtggtgggaa cttctcgtcg
     1201 agcatgacga acatctcctt cttctttccg gatcggcaga agggttgggc gctcggcttg
     1261 aatgccgcgg gggggaacat cggcgtgagc agcgttcagc tcgtggtgcc gctcgtcgtc
     1321 ggctacggct tgctaccggg tagcacccaa aaggtacacc tcgaaaacgc ggggctcatt
     1381 tggctgctcc caatcctcgt cgcgacgata ggagcttggt tttacatgga caacttgagt
     1441 caggctcgct cgagcttcaa agaccaagtc gtcgtcggcc gccgcgcgca gacgtggatc
     1501 atgtctttcc tctacgtcgg aactttcggc tcgttcgtcg ggttctcggc ggcgttcccg
     1561 ctactgttga agacgcagtt cccgagcgtt accgccaaca tcgcgttctt gggagccctg
     1621 gtcggctcgc tgtcgcgccc attcggtggt tggctcgccg acaagttcgg cggggcgcgc
     1681 atcaccttca tcaacttctt cgcgatggcg ctcggcagcg ttggcgtcct cgcggcgctc
     1741 ggacgacacg acatcgtgct gttcctggtg gcgttcctgg cattgttcgt cacgaccggc
     1801 atcggcaacg gttcgacctt ccgcatgatt cccgtcattt ttcgcgccgt gcacggagtg
     1861 gatcagcaga cgggtgaggc gcgcgcggca gctgcggcgc gtgcacggcg tgaaagcgcg
     1921 gccgtgatcg gcatcgcggc cgcgatcgga gcgctgggcg gctacttcat cccgcgttcc
     1981 ttcgcggcct cgatcaaggc ctccggcgga ccgatgctcg ccgtgaagta cttcctcgcg
     2041 ttctatctcc tgtgctgcat cagcacgtgg tggttctaca tgcgacgccg cttcttgacg
     2101 aagctgttgc cgagtcttgc cgaggcaaaa gcgtgactgc actccgtaac cgagtacttt
     2161 cgccgcagcc gaagggcgcg gcaccggggg actacgagtc gggcgtcgtc gtttcggcgc
     2221 ggcgtcgcga gccaggtgag ccgcgccgtc ttccgctcgt gaacgtcgct gccgcgctcg
     2281 cggctcaaca cgagctaacg gccgtggagc gcttttccaa cctgcacgac gaaaacgccg
     2341 tgcccggccg gcagcactat cgcgcgcttc tgccgctgac accgccgcgc tctggcgagc
     2401 aatacgcgtt cgaggtcgat ctcgacgcgt gcacgggctg caaggcgtgc gtcgcggcgt
     2461 gccacaccat gaatgggctc gacggcgggg agaccttccg cgccgtcggc ttgctggtgg
     2521 gcggtacggt cgacgagcct cggcttcaga cggtgacgtc gtcgtgtcac cactgcctcg
     2581 agcctgcgtg tatgagcggc tgccccgttg gcgcctacga gaaggatgcg ctcaccggga
     2641 tcgtgcggca cctcgatgac caatgcttcg gttgtcagta ctgcacgttg atgtgcccgt
     2701 acgatgcccc gaagtacaac gaggcgaaag ggatcgtacg caagtgcgat atgtgcagcg
     2761 gccgcctcgc gcacggcgag gctccggcgt gtgtcgaagc atgcccgaac gaagcgatcg
     2821 cgatccgagt cgtcgaaaag gcggtggttg ctgcggctgc agacgcgcga cacttcgttc
     2881 gcggcgcggc cgatcgcgac aacactctgc cgacgacgag cttcaaaact gcgcgcgcga
     2941 ccccggacaa catgttgccg gtcgactact atcgcgtcgc ggtctctccc gcgcacgcgc
     3001 cgctcgtcgc gatgctgacg ttgtctcagt gggcagtggg cctgacgtgg gcggaggcag
     3061 tattcgcgga ttcgcgcggc tcagtcgtgc tggccagcgt cgaggtgggt gttttgggtg
     3121 tggcgctgct cgctagtttg ctgcatttgg ggcgtccttg gctggcgccg cgcgccgtgc
     3181 tgggagtgaa gacgtcgtgg ctcagccgtg aggtcttggc tttcggtatc ctgatgaagc
     3241 ttgcgttgct gaagctcggc ctcacgctgt tgccgtccat tccgagcttt cacggccgcg
     3301 agctcgtggc cacgctgggt ggcgcattgg tgcaggtcgt gccagcgctc ggcgccttcg
     3361 ccgtattctg ctccgtcatg gtctacgtcg cgacgcggcg ctccccctgg tcattcgcga
     3421 agaccagctt acgctttttc ggtacgacgg cgctgctggg agcggcctca tttctggccc
     3481 tgttcaacgg tcagctcgac ggcgcgtcgc gcgaaacctt ggcccatgcg gtcgcagcca
     3541 tcggcttgct gaagctcggg ttcgacacct ggcttttgct cgaggcgcgt tcgcgtcgct
     3601 tcagcggcga caagcgcatg gcgctcgcca tgctcgggcc gctgggtcgc gtcggatggt
     3661 ctcggttgag ctgtctcgtg gtgggcgcgg ccgtgccgct cacgttgctc gcgtcgaata
     3721 cggtgagcgc ggcgggcttg tggctcggaa cggcgctgct gctcggcgcc gagctcgccg
     3781 agcgctttct cttcttttct gcggcgccga gcctgcgcat gccggggaac ctgcgatgaa
     3841 atcggtagcc gaaggactcc agcagttgaa agacgccatc atccggcgcg acggcccgct
     3901 cacgcgcgag ctcgtgctga agccgagtcg cttcgggctc ggacgcctgc cggagaaggc
     3961 ccagcccgac gcggtgctgc gcaccgtctg cggattttgt tcgacgggct gcagtctcga
     4021 cgtcctcctt caaaagcaga agctcctcgg actcgtcccg agccgcggct acccggtgaa
     4081 tcaaggcgcc gcgtgcccca agggctggga ggcgctcgcg ccgctcacgg gctcgggtcg
     4141 cgcgacgact ccgctcgtgc gccgcgacgg caagctggtc ccggtcagct gggaaacggc
     4201 gctctccgaa atggtgcaac gcttcaaggc gattcaggag gcgcacggtc cggaatcgct
     4261 cgcatggttg ggcaccgggc aaatgccgac ggaggagctc gccttcctcg gcgcgctcgc
     4321 gaagttcggg atgggcatca ttcacggaga cggcaacacg cgtcagtgca tggccactgc
     4381 cgccgtcgcc tacaagcagt cgttcgggtt cgatgcgccg ccgctcgcct acgcagactt
     4441 cgaagagtcc gatgtcatcg tgctcgtggg ttcgaacctc tgcatcgccc accctatctt
     4501 gtggcagcgc gtttgtcaga accgccggtc acccaagatc gtcgtgatcg atccgcgcgc
     4561 gaccgagacc gcgatggccg caacccacca ctttgccgtc aaacccaaag gggaccttgc
     4621 gctgctctac gccgtggcca acgagctcat cgctcgaggc cacatcgacc gcgccttcat
     4681 cgacgcgcac acgacggggt tcgacgattt cgcgcgagag gtcgcgcgct tcacgcccga
     4741 atcggtcagc gccgagagtg gcatcgagcc cgagcgcatc cggcagctcg cgcgtctgat
     4801 tcacgagtcc gagcgagtca gcctgtggtg gacgatgggc gtcaatcaaa gccac
//
LOCUS       DASTKS010000628         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_547935, whole genome shotgun sequence.
ACCESSION   DASTKS010000628 DASTKS010000000
VERSION     DASTKS010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547935"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(363..1505)
                     /locus_tag="VFQ35_12995"
     CDS             complement(363..1505)
                     /locus_tag="VFQ35_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931607.1"
                     /translation="MFSVVPSAMHGTRRLSSARPRFSLLLSLRCRTAALLAALTLAVA
                     PRVEAADDSAAPKNQELTDLDKEDASGPSGAVNARPWAQGISEPRQAAARARFQEANA
                     LLRDSAFANAAQKYAEALSEWDHPGIHYNMALALINLDRPLEVREHLKKATAFGAAPL
                     DQARFDHAQSYLKLIENQLTPLTVQCDVPDAVVRFDDRQLFRGPGRFEGFVLPGEHLI
                     RASKPGFEPTVYQRTLLPGKPMRLHLGLYTQGELTRKLLPGWVPTLVTGVGAAAGATG
                     ALLLLQADSKYKEYDHEISTRPECENGCSPDAGLRALRDSAKRYRTLGTVLAVSGGTL
                     LVGGVTLLIVNAAAAQRESPEELDRRMTWTAVVTPSYVGLSGGARF"
     gene            2183..>2788
                     /locus_tag="VFQ35_13000"
     CDS             2183..>2788
                     /locus_tag="VFQ35_13000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931608.1"
                     /translation="MSRGAPHAVDEAPPVSSSGTFGYAAPSGRIGQYEIIRELGRGGM
                     GVVYLARDERLGRKVAIKVLQVHEPELTRRFVSEARTTALCSHENIVVIHDVGEFEGS
                     PFMVLEYLEGQTLAALLKKSGPLAPSRAVELVAFVVRALVTAHQQGIVHRDLKPENIF
                     ITDSGTVKVLDFGIAKVLNTEWSPSLRGGQLVAPNPQTGLAG"
BASE COUNT          535 a          904 c          877 g          472 t
ORIGIN      
        1 ggagccgcag tgcccgggag agccgttcaa cgaaccgtcg tcgcaaacct ctcctgggtc
       61 gggacgagcg ttgccacaac gttcgtcttg gcaggaaggg ctacagtggt cgccggcgac
      121 gatattgccg tcgtcgcact cctcaccctc gtctttcacc aggttgccgc acgtcccgac
      181 gacgcagatg tcctgcgccg ccgcgcaatg cttgccaact gggcagacgc gcccggacgg
      241 gcatgtgttc gtctccgttt ccgcacagcc tggcgcgctc gcgaaactgc cgacgatgat
      301 tgcagtcggc gcgataaaac gacgccagcg gacccaaagc agaacgcgct cactcgagag
      361 actcaaaacc gtgccccccc actcaaaccg acgtagctcg gcgtgacgac ggcggtccag
      421 gtcatgcgac ggtcgagctc ctcgggcgac tcgcgctgcg ccgcggccgc gttgactatg
      481 aggagcgtga ccccgccaac gagcagagtg ccccccgaga cggcgagcac ggtgcccagg
      541 gtccgatacc gtttcgcgct gtcgcggagc gctcggagcc ccgcgtccgg cgagcagccg
      601 ttctcgcact ccggccgcgt cgagatctcg tggtcgtact ccttgtactt cgagtccgct
      661 tggagcaaga gcagcgcgcc cgtggctccc gccgccgcgc caacccctgt aacgagggtt
      721 ggaacccacc ctgggagaag tttacgcgtg agttcccctt gagtgtaaag cccgagatgc
      781 aggcgcatcg gcttaccggg tagcaacgtg cgctgataca ccgtcggctc gaaaccaggc
      841 ttgctcgcgc gaatgaggtg ctctccagga agcacgaagc cttcgaagcg ccccggcccg
      901 cgaaacaact gccggtcgtc gaaacgaacc acggcatccg ggacgtcaca ttgcacggtg
      961 agcggcgtca gctgattctc gattagcttg aggtaactct gggcgtgatc gaaccgtgct
     1021 tggtcgagcg gcgccgcacc gaatgcggtc gcctttttga ggtgctctcg aacttcgagc
     1081 ggccggtcga ggttgatgag agcgagcgcc atattgtaat gaatgcctgg gtggtcccac
     1141 tcgctcagcg cttcggcgta tttttgagcc gcgttggcga acgccgagtc acgcaacagg
     1201 gcgttggcct cttgaaagcg cgcgcgagcc gctgcctgac gaggctcgga gataccttgt
     1261 gcccatggcc tcgcattgac ggcccctgac ggacccgacg catcttcctt gtcgaggtcc
     1321 gtcaactctt ggttcttggg ggccgctgaa tcgtctgccg cctctacccg cggagcaacg
     1381 gcaagcgtga gcgccgcgag caaagccgcg gtacggcacc gaagtgaaag cagcaaggag
     1441 aaccttggtc gcgccgagga gagccggcgc gtaccatgca tcgcggacgg aacgacgctg
     1501 aacatggagt ttgaataacc cgtgcgatag tcgctgaagc gactcgcaaa gaaggcgaac
     1561 gcaccttacc agggtcagtg tcgtatcgaa aagacaatac gagcctcggc gagctctcgg
     1621 ttcggcgaac ggcaaccgcg tactcgcgat tgcttcgtcg ctgacgtccg acatgaggca
     1681 tcgtcatcgc gacgacggaa tcctcctcac ccagaaagag ccgctgccac ttcgattccg
     1741 gccggcggga ttcgccggac agctcgcaca acgtcgagag cagtcacgcg gagtcgcaaa
     1801 ccagtgattc gcctctcgag tcgctgcctg cgctgaacga agccgtcctc tcccgcggtc
     1861 atcggtgtag agtgtatcga gtgcttcccg cacgctgtcg tcggccggcg acagacacaa
     1921 atcagaacag gtgtatttcc tcatgggaac ggcacgcgaa ggaaccgacc acgaaatcgc
     1981 ctccgactcc gagccgaggg agcggccaca ctcggacgtg gcgccccgca gccgagagac
     2041 ggtgaggacc ggcgaatcac cggaccgcgc atcgcacgag cgacttggac ccggcgctta
     2101 taacgcgcgc acagcgaccg cttttggcgt tcccattccg gctggcgctt acgcgggcca
     2161 gactccgtcg aagaccgcga cgatgtcacg cggcgcgccg cacgcagtcg acgaggcgcc
     2221 accggtttcg agtagcggca ccttcggtta cgcggcccct tccggcagaa tcggacagta
     2281 cgaaataatt cgggagctcg gccgcggagg catgggtgtc gtctatctgg cgcgcgacga
     2341 gcggctcggc cgaaaagtcg caatcaaggt gctgcaagtc cacgaaccgg agctgacgcg
     2401 gcgattcgtc agcgaagcgc gcacgaccgc gctttgcagt cacgaaaaca tcgtcgtaat
     2461 tcacgacgtc ggcgagttcg agggcagccc gttcatggtc ctcgaatatc tcgaggggca
     2521 gacgctggcc gccctgttga agaagagtgg ccccttggct ccctcgcgcg cggtcgaact
     2581 cgttgcgttc gtggtgcgcg cgctcgtcac cgcgcaccag caaggaatcg ttcatcgcga
     2641 tctcaagccc gagaacatct tcatcaccga ttccggcacg gtaaaagtgc tggatttcgg
     2701 gattgcgaag gtgctgaaca ccgagtggtc gcctagcctt cgcggcggtc agctagtcgc
     2761 gcccaacccg caaaccgggc tggccggc
//
LOCUS       DASTKS010000629         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_769916, whole genome shotgun sequence.
ACCESSION   DASTKS010000629 DASTKS010000000
VERSION     DASTKS010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769916"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFQ35_13005"
     CDS             complement(<1..181)
                     /locus_tag="VFQ35_13005"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 family protein"
                     /protein_id="HET9931609.1"
                     /translation="MNNTFEFVVRSVVIGSGATVTMDLWGAALRRLGIPSLDFALLGR
                     WLAHMPQGQFRHQSIA"
     gene            complement(212..907)
                     /locus_tag="VFQ35_13010"
     CDS             complement(212..907)
                     /locus_tag="VFQ35_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931610.1"
                     /translation="MKSQTAVTLTTLARFSFAAAFALSVPLIPAVAAAADGDLASRAG
                     EAAWLAPAAALEARSNDVPTSGASRPELAAVPRADDGTQPPTPALREGKYTGFSLNFT
                     PVLIAPHNDERLGGGVDPELKYTLDRGAVRLSAGLRVGGYYARNLFGITAMPTLRLVV
                     PVGPVEPYVSFGMGYGWLPKLEHSGIATMSRLGVVFRFSENFGLGVEGTIQKIDRSRF
                     EFPSFGSAMTLNF"
     gene            complement(904..1371)
                     /locus_tag="VFQ35_13015"
     CDS             complement(904..1371)
                     /locus_tag="VFQ35_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9931611.1"
                     /translation="MQTLRPLVTGTDFSDCAEEALEVALGLAGAAGTGLTLVHVCELS
                     VDETDEQQLYRCREMLATVVAKHRGRGVEITAVLRSGRPWEKLDNLAVEVGASLIVIG
                     RYGAGGVGLELGTVADHLVRSASRPVLTVSCHLTRFDDEADENTNQRAKENQS"
     gene            1500..>2237
                     /locus_tag="VFQ35_13020"
     CDS             1500..>2237
                     /locus_tag="VFQ35_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9931612.1"
                     /translation="MVVFPPFRLDSDQERLWKGDKQLVLRRKPFAILRYLAEHPKKLV
                     TAEELLAHVWRGAVVSDSAMRSHLHELRQVLGEGVIETVIGRGYRFIAELSDEAVPAP
                     EAPRAAVIDPLVVGRDTELETLRRAFEVARGGRRQVCFVTGKAGIGKSTLVRTFLAGL
                     DPSTVIVARGACFEQHGTPEPYVAIIEAVTTLARSPRGPQALAVLVRYAPTFVAQAPH
                     LVSDEQLVEITRRAAGSSESRQLRELAE"
BASE COUNT          385 a          701 c          762 g          389 t
ORIGIN      
        1 gtgcgatgct ttggtgacgg aattggcctt gcggcatgtg cgcgagccaa cgcccgagca
       61 gcgcgaaatc gagcgacgga atgccgagcc gcctcaacgc tgcgccccac aagtccatgg
      121 ttacggtggc gccgcttccg atgacgacgc tgcgaacgac gaattcaaag gtgttgttca
      181 tggtcatctc cgtagaggta gagacgcgcg cctagaagtt gagagtcatc gccgagccga
      241 acgacgggaa ctcgaagcga gaacggtcga tcttttggat cgtgccttcg acgccgaggc
      301 cgaagttctc gctgaagcgg aagacgacgc cgagtcgcga catcgtcgcg atgcccgaat
      361 gttcgagctt cggcagccag ccgtagccca tgccgaacga cacgtagggt tcgaccggtc
      421 ctaccggtac gaccaagcgc aacgtcggca tcgccgttat gccgaagaga ttccgcgcgt
      481 agtacccgcc gacgcgcagc ccggcgctca aacgcaccgc accgcggtcg agggtgtact
      541 tcagttcggg atcgacgccg cctccgagac gctcatcgtt gtggggagcg atgagcaccg
      601 gcgtgaagtt caggctaaag cccgtgtatt tgccttcgcg tagcgccggc gtggggggtt
      661 gggtgccgtc gtcggcacgg ggaacggcgg cgagctcggg acgagaagcg ccgctcgttg
      721 gaacgtcgtt cgagcgagcc tcgagcgccg cggctggcgc tagccaggcg gcttctccgg
      781 cgcgggacgc caagtcgccg tctgccgcgg ccgcgaccgc ggggatgagc ggaaccgaga
      841 gcgcgaaggc tgcggcgaaa gagaagcgag cgagagtcgt caaggtcaca gcagtttgcg
      901 atttcatgat tggttttcct ttgctcgttg gttggtgttt tcgtccgctt cgtcatcgaa
      961 gcgggtgagg tgacagctga ccgtcagcac cgggcggctc gcggagcgaa ccaagtggtc
     1021 tgcgacggtg ccgagttcga ggccgacgcc gccagcgccg tagcggccga tcacgatgag
     1081 gctcgccccg acttcgaccg cgaggttgtc gagcttctcc cacggcctgc cgctgcgcag
     1141 gacggccgtg atctcgacgc cgcgaccgcg atgcttcgcg acgacggtcg ccagcatctc
     1201 gcggcagcga tagagctgct gctcgtcggt ttcgtcgacg ctaagctcgc acacgtgtac
     1261 gagcgtgagc cccgtgcctg cagcgccagc gagcccaagc gccacctcga gcgcctcttc
     1321 tgcgcagtcg ctgaagtcgg tgccggtgac gagcggtcga agcgtttgca tgggaatcac
     1381 catgccgaca gcactcgtcc actgctgttg gatcaccgtt ggatccgagc cgcggcgtcc
     1441 aatcggtgtt ggatagcgct ctggcctctc gctgacctac gggttaaaac ggcccctaca
     1501 tggttgtttt cccgccattt cgcctagata gcgaccagga gcggctgtgg aagggcgaca
     1561 agcagctcgt ccttcgccgc aagccgttcg cgattttgcg ctacctggcc gagcatccga
     1621 aaaagctcgt cacggcggaa gagctgctcg cccacgtctg gcgcggtgcg gtggtgagcg
     1681 acagcgcaat gcgcagccac ctccacgagc tgcgacaagt cctcggcgaa ggcgtgatcg
     1741 aaacggtcat tgggcggggc tatcgcttca tcgccgagct gagcgacgag gccgtgcctg
     1801 cgccggaagc gccgagagct gccgtcatcg atccgctcgt ggtcgggcgc gacacggagc
     1861 tcgagacgtt acgccgcgct ttcgaagtcg cgcgtggagg tcggcggcaa gtttgcttcg
     1921 tcacgggcaa ggcggggatc ggcaaatcga ccctcgtgcg aacgttcctc gccggcttgg
     1981 atcccagcac cgtcatcgtg gctcgcggtg cctgcttcga gcagcatggc actccggaac
     2041 cgtacgtcgc gatcatcgag gccgtcacga cgctcgcgcg ttcgcctcgt ggcccgcaag
     2101 cactcgcggt gctcgttcgt tacgcgccga cgttcgtcgc gcaagccccg cacctggtca
     2161 gtgacgagca gctggtcgag atcacgcggc gggcggcggg tagcagcgag tcgaggcagc
     2221 ttcgggagct cgcggaa
//
LOCUS       DASTKS010000630         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_324239, whole genome shotgun sequence.
ACCESSION   DASTKS010000630 DASTKS010000000
VERSION     DASTKS010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324239"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFQ35_13025"
     CDS             complement(<1..888)
                     /locus_tag="VFQ35_13025"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9931613.1"
                     /translation="MAATSSGSRAKRRAERLRPLALFAALSLLLVRLPARGAEPAPRP
                     IGVVDLHVDLSYQLNYKGAEFGRGTGQFPADELLRAGVVGVVLPLFVPARVSPTGPRR
                     SDLEDSYRRVLAAIPRVAPYSAPGCGVGTGQVRTFLAFEGAAPLADDPVLLDEWATRG
                     LRSVGLVHTRNNALAGSSGEPSSKGSGLTRAGLALVQRAAALGLPIDVSHASDQATRD
                     VLTAARETKSVVIATHSNARALTNVPRNLADAELRGIAQTGGVIGVNFHSRFLAKGRR
                     ATLDDVVAHIRYLVRIVGIE"
     gene            complement(845..1414)
                     /locus_tag="VFQ35_13030"
     CDS             complement(845..1414)
                     /locus_tag="VFQ35_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9931614.1"
                     /translation="MARTPSTMLPLDTELPAFSLPDTTRDRVVSSGQYRGRPLVVAFI
                     CNHCPFVKHIRSELARFGRYCDERGVGMVAISSNDVDGYPDDSPPNMAKEAREAGYTF
                     PYLYDESQAVAQAFHAACTPDLYLFDSNGRLAYRGQFDDSRPGNGVEVTGRDLYRAVD
                     ALLAGGRPSTEQKASIGCNIKWKSGKAPG"
     gene            complement(1490..>3266)
                     /locus_tag="VFQ35_13035"
     CDS             complement(1490..>3266)
                     /locus_tag="VFQ35_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9931615.1"
                     /translation="TELELAETLLSDEAHRADDAKEALSLARRAGAVLVASKADQQRA
                     GELAARALALLAPADQARLAAPSLEEHVLSLSTLVDAREYERAAREAHELLDGLKDQD
                     RFSPAACDARLLEAKALSSLRRSESALDALAEAREHCAAEPERGARIFYLSGRYASSS
                     SKYTDAIRYFEEVEKRFPAHSLADDARYNAALAHEELGAEARATELLLSLPEAYPNGD
                     MAPEGLFRLALRSLERSEWATALGPLTRALEFLGERDAARGFELSGRERYFHARTLMM
                     MDQRALGLDELESLVKHRPLSYYMLQAYSVLSREAPGRGDQAIAAAEADANSLPFRIV
                     AGQELATPAFSRMMELLRIGEVDTALRELNALGFRNESVGSEILWGVALLYERAGASR
                     FSAELTKLRLSEVSGRWPFGQWAKAWEIAFPRPFLAVVEREAAAAGIDPALAYAIMRE
                     ESAFDPEAVSPANAYGLMQLIVPTAKRAARGTNFVATPSTLKRPRVNVALGCRELARL
                     AGMFEQNPLLAVPAYNAGPGKAAAWLRDRPSVDFDLWVERIPYLETRRYTKRVLSSRA
                     IYGFLYDRAQATSRLTLPKKVSGSG"
BASE COUNT          557 a         1112 c         1108 g          489 t
ORIGIN      
        1 ctcgatacca acaatgcgca cgagatagcg gatgtgcgcg accacgtcgt cgagcgtcgc
       61 gcgccgccct tttgccaaga accgggagtg aaagttcacg ccgatcacgc cgccggtttg
      121 ggcaatgccc cggagctctg cgtccgccaa gttgcgcgga acattcgtca gcgcgcgcgc
      181 gttcgagtga gtcgcgatga cgactgactt cgtctcgcgc gccgccgtga gcacgtcgcg
      241 cgtcgcttgg tccgaggcgt gagagacgtc gatcgggagc cccagagccg ccgcccgctg
      301 gacgagagcc agccccgcgc gcgtgagccc cgaacccttc gagctcggct cgcccgaaga
      361 cccagcgagc gcgttgttgc gggtgtgcac gagcccaacg ctgcgcaggc cgcgcgtagc
      421 ccactcatcg agcaggaccg ggtcgtcggc gagaggcgcc gcgccttcga acgcgaggaa
      481 cgtccgcacc tgacccgtgc cgacgccgca gcccggagcc gaatacggcg cgacccgagg
      541 gatggccgcg agcacgcgcc gatatgagtc ctccaaatcg ctgcggcgcg gtcccgtggg
      601 cgacacgcgc gccggcacga acagcggcaa cacgactccc accaccccag cgcgcaggag
      661 ttcgtcggct ggaaattgcc ccgtaccgcg gccgaactcg gctcccttgt agttcaactg
      721 gtacgagagg tcgacgtgca gatcgacgac gccaatgggg cgaggagcag gctccgctcc
      781 tcgcgctgga agccgcacca agagcagtga aagcgccgcg aacagcgcga gcggacgcag
      841 ccgctcagcc cggcgctttg cccgacttcc acttgatgtt gcagccaatg ctcgcctttt
      901 gctcggtgct cggtcgacct ccagccaaca gcgcgtcgac cgcgcggtac aagtcgcgcc
      961 cggtcacctc gactccgttg ccaggacgac tgtcgtcgaa ttggccgcgg taggcgagcc
     1021 gcccgttcga gtcgaatagg taaaggtcgg gagtgcacgc ggcgtggaac gcctgtgcca
     1081 ccgcctggga ctcatcgtaa aggtacggga aggtgtagcc ggcttcgcgc gcttccttcg
     1141 ccatgttggg cggcgagtcg tcggggtacc cgtcgacgtc gttcgagctg atcgcgacca
     1201 tgccgacccc gcgctcgtcg cagtagcgcc cgaagcgcgc gagctcgctg cgaatgtgct
     1261 tcacgaacgg gcagtggttg cagatgaacg cgacgaccaa agggcgtccg cggtactgcc
     1321 cggagctcac gacgcgatct cgcgtggtgt ccggcaagct gaaggcgggc agctcggtat
     1381 cgagcggcaa catggtggaa ggggtgcgag ccatggcgca atgctaatac cagcgctcgc
     1441 ggtttccagc cctgggcgac tcggccgcgg agggcaatcc cgggcgcggc tagcccgatc
     1501 cgctgacttt cttgggcaac gtgagccgcg acgtcgcttg cgcccggtcg taaaggaagc
     1561 cgtagattgc gcggctcgag agcacccgct tcgtgtagcg ccgcgtctcc aagtagggaa
     1621 tccgctcgac ccacagatcg aagtccacgc tggggcgatc gcgcagccat gccgcggcct
     1681 tccctgggcc agcgttgtac gcaggcactg ccaagagcgg gttctgctcg aacatcccgg
     1741 cgagacgcgc gagctcgcga cagccgagcg ccacgttgac gcgcgggcgc ttgagcgtgc
     1801 tgggcgtcgc aacgaaattg gtaccccgag ccgcgcgctt cgcagtcgga acgatgagct
     1861 gcatgagccc gtacgcgttc gccgggctca ccgcctccgg atcgaaggca gactcttcgc
     1921 gcatgatcgc ataagcgagc gcggggtcga tgccggccgc ggccgcctcg cgctcgacga
     1981 ccgcgaggaa cggccgaggg aaggcgattt cccatgcctt cgcccattgc ccgaagggcc
     2041 agcggcccga gacctccgac aagcgtagct tcgtgagctc ggccgagaaa cgcgatgcgc
     2101 ccgcgcgctc gtagagcaac gcgactcccc acaatatctc ggagcccacc gactcgttac
     2161 gaaagccgag cgcgttcagc tcgcgcagcg ccgtgtccac ctcgccgatg cgaagcagct
     2221 ccatcatgcg cgaaaacgcg ggcgtcgcta gctcctgccc tgccacgatg cgaaacggca
     2281 acgagttcgc gtccgcttct gccgccgcga tcgcctgatc gccgcgaccc ggcgcttcgc
     2341 gcgagagcac cgagtacgct tggagcatgt agtacgagag ggggcggtgt ttgaccaagc
     2401 tctcgagctc gtccaacccg agcgctcgct ggtccatcat catgagagtg cgagcatgaa
     2461 agtagcgctc gcgccccgag agctcgaagc cacgcgcagc gtcgcgctca ccgaggaact
     2521 cgagcgcgcg ggtgagcggc cctagcgccg tcgcccattc gctccgctcg agcgagcgca
     2581 gggccaggcg aaatagccct tccggcgcca tgtcgccgtt cgggtacgct tccggcaacg
     2641 acagcaagag ctcggtcgcg cgtgcctcgg ccccgagctc ctcgtgagcg agcgcggcgt
     2701 tgtagcgcgc gtcgtcggcc aacgagtgtg ccggaaagcg cttttcgacc tcttcgaagt
     2761 agcggatcgc gtcggtgtac ttcgagctcg acgaggcata gcgccccgaa aggtaaaaga
     2821 tgcgagctcc gcgctcgggc tccgcggcgc agtgctcgcg tgcctcggcg agtgcgtcga
     2881 gcgccgactc cgaacgacgc aagctcgaca atgccttggc ttcgagcaga cgggcatcgc
     2941 aagccgcggg cgaaaagcgg tcctggtcct tgaggccatc gagaagctcg tgagcttcac
     3001 gcgccgcgcg ctcgtattcg cgcgcgtcga cgagcgtgct gagcgagagc acgtgctcct
     3061 cgagcgacgg cgcggccaag cgtgcctggt cggccggggc gagcagagcg agggcgcgcg
     3121 ccgcgagctc accggcgcgc tgctggtccg ccttgctggc gacgaggacc gctcccgcgc
     3181 gacgagccaa cgagagcgcc tcttttgcgt cgtcggcgcg gtgcgcctcg tccgagagca
     3241 aggtttcggc gagttcgagc tcggtg
//
LOCUS       DASTKS010000631         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_548117, whole genome shotgun sequence.
ACCESSION   DASTKS010000631 DASTKS010000000
VERSION     DASTKS010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548117"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="VFQ35_13040"
     CDS             <1..1922
                     /locus_tag="VFQ35_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931616.1"
                     /translation="GLGAGAGMGLSARGTLSATSSVSARPNKAALKGTATLSEINVQG
                     GTISNAARVVASMRAGFRACYNRGLGEDPDSEGTLRVTMLVAPQGHVIRTDLGATDSL
                     PANVLSCIRARAQAAQFDAVAEGVPSRVSVTLTLATDSSPALERSDPLAVESPPVTPS
                     RKSAPNASDASAPMGVVRHQVRPCGPGANLPLAERRILWRERLSTASSASLALTIYRR
                     ALEACEATHWDERALLLVSIVDRLSTITDRVELWRALLQVSPAAADAVYRFMMLRVQT
                     AEDLKQLHEALGLTRIEPTLLAALLKKAKDPVERLSLLRGAAERFPDDTELALVVLDA
                     YEDAHDDAGGRAFARALRRRVDATAHVRTQVGEYYLRLASREQEPFAARDAEEARRTF
                     GELVEFASEDPLARRRLGDLLRAHGWFEEAARQYETLSELTPDDASVQLLRALAANGM
                     GRVEEAVRWTEKAASTGAEDGTSPLSLAARAQASTFLSWARSAALAAGRTAEVERLRT
                     RAARISAREASHVRFTLTWAHPELRPALWTVASGSAAPSPENLPLYGIAQAFVAEPAL
                     VEVRLDPEDAGNAARLDTKAVLTAVRGEGTPDERIARAEVSFRDGNGKPLDRIEARFE
                     NGELVVGAPARKEAP"
     gene            1919..>3064
                     /locus_tag="VFQ35_13045"
     CDS             1919..>3064
                     /locus_tag="VFQ35_13045"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9931617.1"
                     /translation="MKELKPWHVVVVSALLLGVGALLVLRNIANTPKFSIAQTVADVH
                     AEHAPVRVGSAEIRGHARLVDGSEVSTEAGGRARVRLDDGSLVVVDEGTRFRLRGQKL
                     KLSAGRIFVQGAAGAHTQVELDDVSTEVSGSSAAFALTKSSRSVYCAQGELLLRRGST
                     QTHVAAGERAVLEASKLEVSPETAFDDWTGGLAVPWSSHGAGRALVPELFTREADGAG
                     QSLSIRSEKIDVSIEGEFAITRTRTSYFNGSDHAVAPQVRVALPESAIISRVARATGS
                     EEIEAKIAIQRPGEGGAFPSTPSRLEWAGDGWMRGVLPSIESGASVDVLLTYAEWIGT
                     HAGQRTYRFPLASDDPPLIGELRAAVHASGDARLLSTNAGAATTSRHI"
BASE COUNT          489 a         1011 c         1074 g          490 t
ORIGIN      
        1 ccggtctggg cgcaggtgca ggcatggggt tatccgcgag ggggacgttg tcggccacca
       61 gttccgtgtc ggcgcgaccg aacaaggcgg cgctgaaggg cactgcgacg ctgagcgaga
      121 tcaacgtgca ggggggaacg atttcgaatg cagctcgcgt cgtcgcgagc atgcgcgctg
      181 ggtttcgcgc ttgctacaac cgcggcctcg gcgaagaccc agacagcgag ggcacgctgc
      241 gagtcaccat gttggtcgcg ccgcaaggtc atgtcatcag gaccgacctc ggcgctaccg
      301 attcgctgcc cgcgaacgtg ctttcctgca ttcgtgcgcg cgcgcaggcc gcgcaattcg
      361 atgccgtcgc cgaaggggtt ccgtcgaggg tctcggtcac gctcaccttg gcgaccgatt
      421 cgtcacccgc tctcgaacgc agcgacccgc tcgcggtcga gtcgccgccg gtcactcctt
      481 cgcgtaagtc ggcgcccaac gcgagcgacg catcggcacc aatgggcgta gtgcgtcacc
      541 aagtgcggcc ctgcggtccc ggcgcaaact tgccgctcgc cgagcggcgc attctgtggc
      601 gcgaacggct gtcgaccgcg agctccgcaa gccttgcgct cacgatttat cgcagagcgc
      661 tcgaagcctg cgaggcaacg cattgggacg agcgtgcgtt gctgctcgtc agtatcgtcg
      721 acaggctctc gacgatcacg gacagggtcg agctctggcg cgcgctcctg caagtgagcc
      781 cggcggcggc ggacgcggtg tatcgcttca tgatgcttcg cgttcagacg gcagaagatc
      841 taaagcagct ccacgaggcg ctcggtctca cgcggatcga gccaacgctg ctcgctgccc
      901 ttttgaagaa ggcgaaggac ccagtcgagc gactgtcgct gctacgcggg gccgcggagc
      961 gcttccccga tgacaccgag ctcgcgctgg tcgtgctcga cgcgtacgag gacgctcacg
     1021 atgatgcggg aggccgagcg ttcgcgcggg cgttgcgacg gcgggtggat gccaccgccc
     1081 acgtaaggac gcaggtgggt gaatactact tgcggctcgc gtcgcgcgag caagagccgt
     1141 tcgccgcccg cgacgccgag gaggcgcggc gcaccttcgg cgagctcgtc gagtttgctt
     1201 cggaggatcc gctcgctcgt cggcgcttgg gcgacctgct gcgcgcgcac ggctggttcg
     1261 aagaggcagc gcgccagtac gaaaccctga gcgaactcac gccagacgac gcgagcgtgc
     1321 agctcttgag ggcgctcgcc gcgaacggaa tgggccgcgt cgaagaagcc gtgcgctgga
     1381 cggagaaggc ggccagcacc ggcgccgaag acgggacgag cccgctctct ttggcggctc
     1441 gcgcccaagc gagcacgttc ctctcctggg cgcgcagcgc cgcgctcgcg gcgggacgca
     1501 ccgccgaagt ggagcgcttg cggacacgcg ccgcccgaat ctccgcgcgc gaagcttcgc
     1561 acgttcgctt tacgctgacg tgggcacacc cagagcttcg tcccgcgctg tggaccgttg
     1621 cttcgggctc ggccgcgcct tcgccagaga acctccccct ctatggcatt gcgcaggcgt
     1681 tcgttgcaga acccgcgctc gttgaggtgc ggctcgaccc cgaagacgcc ggcaacgccg
     1741 cgcggctcga tacgaaagca gtgcttacgg ccgtgcgcgg agaaggcacc ccggacgagc
     1801 gcattgcgcg cgcggaggtg agctttcgcg acggcaacgg caagccgctc gaccgcatcg
     1861 aggctcgctt cgaaaacggt gagctcgtag ttggcgctcc cgcgcgcaag gaggcgccgt
     1921 gaaagagctg aagccttggc acgtcgtcgt ggtctccgcg ctgttgctcg gggtcggcgc
     1981 gctgctcgta ctgcgaaata tcgcgaatac gccgaagttc agtatcgcgc agacggtggc
     2041 ggacgtgcac gccgagcatg cgccagttcg agtcggcagc gccgaaattc gcggacacgc
     2101 acggttggtc gacgggtcgg aggtgtcgac cgaggcgggc ggtcgcgctc gcgtgcgtct
     2161 cgacgacggc tccctcgtag tcgtcgacga gggtacccgc ttccgattgc gaggacagaa
     2221 gctgaagctc tcggcgggga gaatcttcgt tcagggtgcg gcgggtgccc acacgcaagt
     2281 cgagctcgac gatgtttcga cggaggtctc ggggagttcg gctgcgttcg cgctgaccaa
     2341 gtcgtcacgc tccgtttact gtgcgcaggg cgagctcctg ctccggcgcg gctccacgca
     2401 gacgcacgtc gccgcgggcg agcgcgcggt gctcgaggct tcgaagctcg aggtctctcc
     2461 cgaaacggcg ttcgacgatt ggacgggcgg actcgcggtc ccttggtcga gccatggcgc
     2521 gggccgcgcg ctcgtgcccg agctcttcac gcgtgaggcg gacggcgcgg gccagtcgct
     2581 ctccatacgc tccgaaaaga tcgacgtgag catcgagggg gagttcgcga tcactcggac
     2641 gcgcacgagc tacttcaacg gctcggatca cgcggtcgcg ccccaagtgc gcgttgccct
     2701 ccccgaatct gccatcatct cgcgcgtcgc gcgggcgacc ggaagtgagg agatcgaagc
     2761 caaaatcgcg attcagcgac ccggcgaggg cggcgcgttc ccctcgactc cttcacgcct
     2821 cgaatgggcg ggagatggct ggatgcgtgg cgtgctccca tccatcgagt cgggagcgag
     2881 cgtcgacgtg ctcttgacgt acgccgagtg gatcggcacg cacgcggggc aacgcacgta
     2941 tcgcttcccg ctggcgagcg acgacccccc gctcatcggc gagcttcgcg cggcggtcca
     3001 cgcgagtggt gacgcgcgcc tgctgtcgac gaacgcggga gccgccacga cttcgcggca
     3061 catc
//
LOCUS       DASTKS010000632         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_102101, whole genome shotgun sequence.
ACCESSION   DASTKS010000632 DASTKS010000000
VERSION     DASTKS010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102101"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..>2118)
                     /locus_tag="VFQ35_13050"
     CDS             complement(327..>2118)
                     /locus_tag="VFQ35_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9931618.1"
                     /translation="GACHPHKPRLERRTAKLIGVRGTLAGALLLSACGPSFERSPSHT
                     ASLESVSLIVSEVAQSTAFGGATADKVEVYCTNSSGCAAFKVCDSAPSGSSCSALQPA
                     LASGARTVVSRGASITTSDQVWLADATGAELPGTRVGPFSCDAGSSQSRADCSIAAFS
                     ACGAPTLGTSAGSCAAGDFPEAFSASVRFTQNQHGGPEPTCNRALCQELLQAIDGAET
                     SIDFAIYGMRAQPAILNALVQAQSRGVTVRGVVDTENSTGASFGYPDTAALISSLGSN
                     NVHPDVGPGYSYIMHNKFFVFDRASVWTGSTNISDTELGGEYNSDVAVLLSSYRIAEI
                     YEAEFAQMFGGAFHNRKTDDTEHVISPSHFSDGTQVRSYFSPTDHAVDNAVIPLVDQA
                     EHTLDIAMFYFTSTPIASAVLSAKARGVAVRMVLDASGASNAYSQHTTLCQNGVAVKV
                     ENWGGKSHSKWAIADAGYPGAAVVFGSMNWTRAGDEQNDENTLYVRNTAFANAFHTEF
                     ERQWADLASVPMCSSVSVEGADSSQCNASNDCHLSCSSGSCCDGIDNDYDGKIDLQEE
                     ACGCADGIDNDSDGYIDAADFDCQPPVDDP"
BASE COUNT          324 a          647 c          746 g          401 t
ORIGIN      
        1 gggtggccgg cagctgaacg gcgattcgcg attcgaccct gcccgggcgg cgcggttcgg
       61 gctggcggcg cgttcggggc gcggcgcgtt tcgggaacgg cgcgtttcgg gaacggcgcg
      121 ttcggggcgc ggtgcggttt gggaacggcg cgtttcggga acggcgcgtt tcggaacggc
      181 gcggttcggg aacggcgcgg ttcggggcgc ggtttgggaa cggcgcgttt gggaacggcg
      241 cgttcgggaa cggcgcgttc gggaacggcg ggtgcggttt gggactaggg tggtgggggg
      301 gcgtgcgaag ggtcgtggtt agcgggtcag gggtcgtcca ctgggggctg gcaatcgaag
      361 tctgcggcgt cgatgtatcc gtcgctgtcg ttgtcgatgc cgtcggcgca gccgcacgct
      421 tcctcttgga gatcgatctt gccgtcgtag tcgttgtcga tgccgtcgca gcaggagccg
      481 ctgctgcagc tcaggtggca atcgttgctg gcgttgcatt gcgacgagtc ggcgccctct
      541 accgacacgg agctgcacat cggcaccgag gcgaggtcgg cccactgtcg ctcgaattcg
      601 gtgtggaaag cgttggcgaa ggcggtgttt cgtacgtaca gcgtgttctc gtcgttttgc
      661 tcgtcaccgg cgcgcgtcca gttcatcgag ccaaacacga ccgccgcgcc cgggtagccg
      721 gcgtcggcga tcgcccattt cgagtgagac ttaccgcccc agttttcgac tttgacggcc
      781 acgccgttct ggcagagcgt agtatgctgc gaatacgcgt tcgacgcgcc gctcgcgtcg
      841 agcaccattc gcaccgccac accccgcgcc tttgccgaga gcaccgcgct cgcgatgggg
      901 gtgctcgtga agtagaacat cgcgatgtcg agcgtgtgct ccgcttggtc gacgagaggg
      961 atcaccgcgt tatcgaccgc atgatccgtc ggcgagaagt agctgcgcac ttgcgtgccg
     1021 tcactgaagt gcgaaggcga gatgacgtgc tcggtgtcgt ccgtcttccg attgtggaag
     1081 gccccgccga acatctgcgc gaattcagct tcgtagatct cggcgatgcg gtacgaggac
     1141 aggagcacgg cgacgtcgga gttgtattcg ccgccgagct cggtatcgct aatgttggtg
     1201 ctccccgtcc agaccgacgc acggtcgaag acgaagaact tgttgtgcat gatgtacgaa
     1261 tagccgggcc caacgtctgg gtgtacgttg ttcgacccga gggagctgat gagcgcggcc
     1321 gtgtcgggat agccgaacga tgctccggtc gagttctcgg tgtcgaccac tccgcgcacc
     1381 gtgactccgc gactttgcgc ttgaaccaat gcattcaaaa tcgcaggttg cgcgcgcatt
     1441 ccgtagattg cgaagtcaat cgaggtctcc gccccgtcga tggcttgcag gagctcctgg
     1501 cacaacgctc ggttgcacgt gggttcgggt ccgccatgct gattctgcgt gaagcggacg
     1561 ctcgcgctga aggcctccgg aaagtcgccg gccgcgcagc tccccgcgct cgtgcccagc
     1621 gtcggggcgc cgcaggccga aaacgcagca atcgagcaat ctgcgcgcga ttgcgacgac
     1681 cccgcgtcgc aggagaacgg cccgacgcgc gtaccgggta gctccgcgcc cgtcgcgtcg
     1741 gcgagccata cctgatcgct cgtcgtgatg ctcgcaccgc gcgagaccac cgtgcgcgcg
     1801 ccgctcgcga gcgcgggctg cagcgcagag cagctcgacc cggatggcgc gctgtcgcac
     1861 accttgaacg ccgcgcatcc actcgagttg gtgcagtaga cctcgacttt gtctgccgtc
     1921 gccccaccaa aagcggtgct ttgcgcgacc tcgctcacga tgagcgacac gctctcgagc
     1981 gacgcggtat ggctcggtga gcgctcgaag ctcggcccac aagcggagag cagcaaagct
     2041 cccgccagcg tccctcgaac gccgatcagt ttggctgttc ggcgttcgag ccttggtttg
     2101 tgggggtggc atgctccg
//
LOCUS       DASTKS010000633         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_324433, whole genome shotgun sequence.
ACCESSION   DASTKS010000633 DASTKS010000000
VERSION     DASTKS010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324433"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /gene="pgl"
                     /locus_tag="VFQ35_13055"
     CDS             complement(<1..532)
                     /gene="pgl"
                     /locus_tag="VFQ35_13055"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9931619.1"
                     /translation="MSRRVERVRVGTSGLPELAAREIAQMLSAAVREHGTATLALAGG
                     TTPRAAYEHLANEAVPWRSVSIFFGDERCVPPDDSDSNYRMAREALFGRLSEGPARVE
                     RMLGESSDHDAAARAYEAVLPDSIDVLVLGVGEDGHTASLFPGSSALTEKTRRVLPVV
                     GPKPPPNRLTITPPVLN"
     gene            586..2163
                     /locus_tag="VFQ35_13060"
     CDS             586..2163
                     /locus_tag="VFQ35_13060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931620.1"
                     /translation="MGRPAFEPQRVLAGRYRLEKRLGGGGMGAIWQAEHIALQSPVAV
                     KLIDRDAVHDEQAVARFLREAKAAAALRSHHVVHIIDYGMDDNVPFIVMELLEGETLA
                     QRLKRVRRLTPTDTARFVSHIGRAMSRAHEAGIVHRDLKPENVFIVHNEDEEIAKVLD
                     FGVAKIERGALAVEGERTRTGSILGTPYYMSPEQAQGNKAVDYRSDLWSLGVIAFECL
                     TGKRPFYSDGLGDLVLQICVRDLPVPSEVAAVPIGFDAWFAKAVARDPEHRFQSAREM
                     TEALRDALCIETRELRNSQPEILVSTGETVLERVSAAPSSARATRPHSDPLAVSRVDS
                     QTSAGDPTPPPPSSQRADPVAATVAFSPMPRERIALSERHSHPPAGSGMGSIVAVAAV
                     ALALGLGGGVWALQHFGIKARTSPLPTVNDSAERVDERVDRPSLKASSRKDTASTDAT
                     AQASASVAPRSAPAPSASASASGREHKEAPSTADAVPAASTKPPSVDGGWVKPAWAIP
                     DPEPKRADDIVDAPPDR"
     gene            2377..>3864
                     /locus_tag="VFQ35_13065"
     CDS             2377..>3864
                     /locus_tag="VFQ35_13065"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET9931621.1"
                     /translation="MNTETNQRNEPPRVVIEQVTPLVDGGRYPVKRQVGATVEVGAAI
                     FKDGHDHISADLVYQGPADREPRRQPLVYRVDPDRWYGSLKVDTLGRYRYWIEAWTDR
                     YATFRADLAKRVDAGQDVRSELLEGAAILSRHLNTLKGEALRRIQDAITKLRDANLSL
                     DERLRVAFAAEIQELVRSAPEAREVTRTTPALEVLVDPEEASFSAWYELFPRSQGAVP
                     GRHGTFKEAARRLPELAALGFDVVYLPPIHPIGRTHRKGRNNSRTAEPGDVGSPWAIG
                     GPEGGHDAVHPELGSLADFQEFVKSAREFGIEVALDFALQCSPDHPWCKEHPEWFFIR
                     PDGSIRYAENPPKKYEDIYPINFWCEDRQGLWNACRDLLLFWIQHGVKIFRVDNPHTK
                     PLSFWEWVLREVNVRHPDVIFLSESFTRPNRMKALGKLGFNQSYTYFTWKNSAWELKD
                     YLTELTRTEMVEYFRPNFFANTPDILHEYLVAGGRPAFRVRLILAA"
BASE COUNT          678 a         1267 c         1270 g          649 t
ORIGIN      
        1 cattcaatac aggtggcgtg atggtgagac gattgggcgg aggcttcggc ccaacgacgg
       61 ggagaacccg gcgcgtcttt tcggtcaaag cgctggaacc tgggaataaa gacgcggtgt
      121 ggccgtcttc ccccacaccg agtaccagca cgtcgatgga atcgggtagg accgcttcgt
      181 aggcccgggc ggccgcatcg tggtcggaac tttctcccaa catcctttcc acccgcgccg
      241 gaccctcgga aaggcgcccg aaaagtgcct cccgcgccat ccggtagtta ctatccgaat
      301 cgtccggagg aacgcaccgc tcgtccccga agaatatcga gacagaccgc cagggaaccg
      361 cttcgttggc gaggtgttcg taggcggcgc gcggcgtcgt tccgcccgcc aaagccagcg
      421 tcgcggtccc gtgctcgcgc accgccgcgg acagcatttg ggcgatttcg cgcgctgcga
      481 gctcgggtag gcccgacgtg cccacgcgta cccgttcgac tctccggctc atttgagcga
      541 gaagtaatgc acggagcgcc cccgagcgct atggtcccga accgcatggg acgccccgct
      601 ttcgagcccc agcgtgtgct ggccggccgc tatcggctgg aaaagcggct gggtggaggc
      661 ggcatgggcg cgatctggca agccgagcac atcgcgctgc aatcccccgt agcggtaaag
      721 ctcatcgacc gcgatgccgt gcacgacgag caagcggtcg ctcgcttctt gcgtgaagcg
      781 aaggcggccg ccgcgctgcg cagccatcac gtcgttcaca tcatcgacta cggcatggac
      841 gacaacgtgc cgttcatcgt gatggagctg ctcgagggag aaactctcgc gcagcgactc
      901 aagcgggtgc ggcgcttgac gccgacggac acggcgcgct tcgtgagtca catcgggcgc
      961 gcgatgagcc gcgcgcacga ggctgggatc gtgcaccgcg atctcaagcc ggagaacgtc
     1021 ttcatcgttc acaacgaaga cgaagaaatc gcgaaggtgc tcgatttcgg cgtcgccaag
     1081 atcgagcgcg gcgcgctcgc ggtcgagggt gagcggacgc gcaccggctc gattctgggc
     1141 acgccgtact acatgagccc cgagcaagcg cagggcaaca aggccgtcga ctaccgctcg
     1201 gatctctggt ccctcggcgt catcgcgttc gagtgcctca ccggaaagcg tccgttctat
     1261 agcgacgggc tcggcgatct ggttttgcag atctgcgtgc gcgacttgcc ggtgccgagc
     1321 gaagtcgcgg cggttcccat cggtttcgat gcctggttcg ccaaagccgt ggcgcgcgat
     1381 cccgagcacc gtttccagtc tgcgcgcgag atgacggagg cgttacgcga cgcgctctgc
     1441 atcgagacgc gcgagctccg caatagtcag ccggagatct tggtttcgac cggcgaaacg
     1501 gtgctcgagc gtgtgtcggc ggcgcccagc agcgcgcgcg cgacgcgccc gcattcggat
     1561 ccgctcgcgg tttcgcgcgt cgactctcag actagcgcgg gagacccgac gccgcctccg
     1621 ccgagctcgc agcgcgcgga tccagtggca gcgacggtcg cgttcagccc gatgccgcgc
     1681 gagcgcatcg cgctctcgga gcggcactcg catcctccgg cgggttcggg catgggcagc
     1741 atcgtcgcgg tcgcggcggt cgcgttggcg ctcgggctag gtggcggtgt ttgggcgctc
     1801 cagcacttcg gtatcaaggc gcgcacctcg ccgttgccga cggtgaacga cagcgccgag
     1861 cgcgtggacg agcgcgtcga caggccctcg ttgaaagcga gctcgcgcaa agacaccgcg
     1921 agcacggacg ccactgctca agcgagcgct tccgttgcgc cacgctctgc ccccgcgccg
     1981 agtgcatcgg ccagcgcgtc gggacgcgag cacaaagagg cgccgtccac cgctgatgcc
     2041 gttcccgcag cgtcgacgaa gccaccgagt gtcgatggcg gttgggtcaa acctgcatgg
     2101 gcgattcccg atcccgagcc aaagcgagcg gacgatatcg tcgacgcgcc gccggatcgt
     2161 tgacggacag acggtttcgc cggcgttgct ggcacgacaa ttcggtggag aaagtgtccg
     2221 ccgacgcgaa agtggcttcg aatcgacggc gcgctttcgg gccggcgcgt accgccccaa
     2281 aaaacggcaa cgcgcgctga tcctttttga gcacgcgctt gacgctttgc ttggtggcga
     2341 cgaaactcgg gctcaaagtc gcgagtttcg agcttcatga acacagaaac gaaccagcgc
     2401 aacgagccgc ctcgagtcgt aatcgaacaa gtcacgccgc tcgtcgatgg ggggcgctac
     2461 ccggtgaaga ggcaggtggg cgccaccgta gaggtcggag cggccatttt caaggacggc
     2521 cacgatcaca tctccgcgga cctcgtttat cagggcccgg ccgatcgcga gccgcggcgt
     2581 cagccgctgg tttaccgcgt cgatcccgat cgctggtacg gctcgctcaa ggtcgacacg
     2641 ctgggtcgct atcgatattg gatcgaagct tggacggacc gctacgcgac gtttcgcgcc
     2701 gatctcgcga agcgagtgga cgcaggtcaa gacgtgcgct ccgagctcct cgagggcgcc
     2761 gccatcctgt cgcgccactt gaacactctc aagggtgaag cgttgcggcg cattcaagac
     2821 gcgatcacca agctgcgcga cgcgaacctc tcgctcgacg agcgcttgcg cgtcgctttc
     2881 gcggccgaga tccaggagct cgtgcgctcc gcgccagaag cgcgcgaagt cacgcgaacc
     2941 acccccgcgc tcgaggtgct ggtcgacccg gaagaggcgt cgttttcggc gtggtacgag
     3001 ctgtttccgc gctcgcaagg cgcggttccc gggcgccacg gcaccttcaa agaggcggcg
     3061 cgacgcctgc ccgagctcgc cgcgctcggc ttcgacgtcg tttacttgcc gccgattcac
     3121 cccatcgggc gtacgcatcg caagggccgc aacaactcgc gcactgccga gcccggcgac
     3181 gtcgggagcc cctgggccat cggtgggccc gaaggcggtc acgacgcggt gcacccggag
     3241 ctcggatcgc tcgcagattt tcaggagttc gtgaagagcg cgcgcgagtt cggcatcgag
     3301 gtcgcgctcg acttcgcgct gcagtgctcg ccggatcacc cgtggtgcaa ggagcacccg
     3361 gagtggttct tcatccgccc cgacggcagc atccgttacg cggagaaccc acccaagaag
     3421 tacgaggaca tctatcccat caacttctgg tgcgaagatc ggcaagggct ctggaacgct
     3481 tgtcgcgacc ttttgctctt ctggatccag cacggggtga agattttccg cgtcgacaac
     3541 ccgcacacca agccgctctc gttttgggag tgggtgctgc gcgaggtaaa cgttcggcac
     3601 ccggacgtga tcttcctgtc ggagtcgttc acgcgcccga accgcatgaa ggcgctcggg
     3661 aagctcggct tcaaccagag ctacacctac ttcacgtgga agaactcggc gtgggagttg
     3721 aaggactacc tcaccgagct cactcgcacc gaaatggtcg agtatttccg gccgaacttc
     3781 ttcgcgaaca cgccggatat cctgcacgag tacttggtcg cgggaggccg gccggcgttt
     3841 cgagttcggc tcatcctcgc cgcg
//
LOCUS       DASTKS010000634         7419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_770275, whole genome shotgun sequence.
ACCESSION   DASTKS010000634 DASTKS010000000
VERSION     DASTKS010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770275"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFQ35_13070"
     CDS             <1..830
                     /locus_tag="VFQ35_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HET9931622.1"
                     /translation="REGNSYVEVPVAADGRADADRLGEAAQDAAAVLVGYPSFYGPLS
                     DLRRVASVAHDRGALLVAVNAEPYALALAESPGALGADIAVGEGQPIACPPQLGGPGV
                     GLFACRDDRQFLQQMPGRIAGETVDVNGQRGYVLTLSTREQHIRRERATSNICTNQGL
                     IALSLTIRMSLLGKRGFVEVAEQCLAKARYLRSAILALPGYAPGFGDAPFFNEFAIKV
                     RGGSAARVIERLESEGIVAGYDLGQVDPAFADRLLVAVTETHERADLDRLVRGLERC"
     gene            complement(886..1515)
                     /locus_tag="VFQ35_13075"
     CDS             complement(886..1515)
                     /locus_tag="VFQ35_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931623.1"
                     /translation="MIGPTWKLDRPHGGLPGTALPWYLAPVSFSSRSTLAGGLVAFAL
                     FSFAPSSARADVSSWAYAGLGPGFLRDDRQRLTLQLDAGIGTPPATLVVGGVFRAQPY
                     FKSGTDLALLARVATRGDVQGGFGLALDAGGYERFWGRHSEGGLAALNLGAPWGITLS
                     VTGGMGTHDERFASFTLGFDFARLTVYRTTGTNWFSNPFATDDRGRGPR"
     gene            1546..2064
                     /locus_tag="VFQ35_13080"
     CDS             1546..2064
                     /locus_tag="VFQ35_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET9931624.1"
                     /translation="MSLRTVREIDWENWRPRDVGTLLFIVRDGEVLLIRKKRGLGAGK
                     INGPGGRLEPGESPLAAAIREVEEEVCVTPSEIEARGELSFDFTDGYRLHAHVFVAFA
                     HAGTPSATPEADPFWCSLEELPYASMWADDALWLPHVLAGRGVRGRFVFEGDEMLDYE
                     LDVFEISSPARP"
     gene            complement(2042..3031)
                     /locus_tag="VFQ35_13085"
     CDS             complement(2042..3031)
                     /locus_tag="VFQ35_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9931625.1"
                     /translation="MPKKEKLALPLADPERLARAESLAEQAPLPARVGRVELGTASWT
                     DRTLIESHSFYPRGASTPEARLRHYAAHFPFVEVDATYYTLMPADTAARWVGFTPADF
                     SFDVKAFPSLTGHPIDVTRLPADLKEAVVAAGHERRVYPDKLPPELLGEIDARFLSFL
                     RPLSDAQRLRAVLLQFPPWFTATRGNVKKLSAIRAAHPALPFAVEFRHKSWFVEERRA
                     RVFDVLRAEQMSFVVIDEPGMPLVAEVTHPELALIRFHGKNERGWKKKGATVHERFGY
                     LYEPSELEAWVKPVKQLASQAERVQAIFNNCVRNYAVLGAKDLAVLLTGEPVS"
     gene            complement(3035..3550)
                     /locus_tag="VFQ35_13090"
     CDS             complement(3035..3550)
                     /locus_tag="VFQ35_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931626.1"
                     /translation="MRRRALTVACVALLLGCGGARGEGPVAVHPPVGEPPEFAFGVLD
                     GTVFTAENTRGRATALLFATSYDLASQLVARRLDEVYRVRKPRFNAACIALEAAENAV
                     LVETFRDTLDLRYPVAIADRVELRSFPAFANIDRVPTLVVLDRRGRERLRVFGPFEAK
                     HLRAWLSAAEE"
     gene            complement(3558..4262)
                     /locus_tag="VFQ35_13095"
     CDS             complement(3558..4262)
                     /locus_tag="VFQ35_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931627.1"
                     /translation="MSESTERRTPTSANLAELTPLPLTGAQRESRLVDAWREWLRALA
                     TDAEAALAAAIAYRELGGEARDNWLKALEQDADLISVPKFAVYAPLLAVESDPERRSR
                     IQRAVGPMESATPSVAAQAFQGVSATGMRVAVLVTPLYLDFAQVLACGYRTDMGFDWV
                     RHDPIVERRRAPAHGESIEGIKLESTPLTQVIDDLAHAVLAHRRSGRDLPEALRVFAD
                     WFGPAPGGSTPPPPDT"
     gene            complement(4259..4648)
                     /locus_tag="VFQ35_13100"
     CDS             complement(4259..4648)
                     /locus_tag="VFQ35_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931628.1"
                     /translation="MPVPPLLDADLVARRLRVRARDAVFVKGVLEASEGVGVLFAEQG
                     GDLVVAAPRSLEAALVAIVHDLALELDGVVEPDPREGIAGPGSQVEKVSESRRASPDG
                     DVRVAAERDPVLLSTSERPTARLFSKP"
     gene            4743..6179
                     /locus_tag="VFQ35_13105"
     CDS             4743..6179
                     /locus_tag="VFQ35_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931629.1"
                     /translation="MKESSDDLAPGSLPPLDKSEDAELDDAALGGLLDPLPTLDGSSD
                     DGDASALDTGVETFDILADNVEDAATTIDFGGNFADLLVLPSENEESDDSVGLAAADP
                     DFVNAPEVPEPRDDEEGLDEPGLGLGSLPELVQGEDDEGVDDTGELPASARFGDEPRP
                     AVAARAWALAGPELKLEPCAAIASIDGIVVAASSDLFWFSPGNLTPVRLEAGSSHVHS
                     IALLGGGWDYAACATASGKLIRRGRLASASEELRRVREELGSPGREVFDLCQPGPAFP
                     HTLLLRTASGKLLRSDDDGVTFRRVSEHKVVALAPHGPPALALSAEGVLLSSDDGAGS
                     FREVKLQGLAARVALAKSPLIAGNAKAVLLGDSSLGVLLSKDRSEHFVRLPGTLGVSA
                     LCMGGDAESPCGFAAVYDDARDHTLLLRIDLATFGVSTIASIDADSSDEDEMTEGARV
                     AALVWDNVYGRLWAAGGFGVKAYAPTDG"
     gene            complement(6189..6560)
                     /locus_tag="VFQ35_13110"
     CDS             complement(6189..6560)
                     /locus_tag="VFQ35_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931630.1"
                     /translation="MFPKLVIASVFAFGALSCKDNAECDKARTALAKSWHGLAESAAR
                     RQLGGVDIEGWKFVQGRAQLLESSFMTTQVTWDSADKARKEIADRVAGLQSDAPANVE
                     GFRLSLKAALDEQDAFAKKCR"
     gene            6731..>7419
                     /locus_tag="VFQ35_13115"
     CDS             6731..>7419
                     /locus_tag="VFQ35_13115"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9931631.1"
                     /translation="MAIVVQKFGGSSLADVERVGHVADFVKRTSQSGSKLIVVVSAMG
                     KTTENLLDLARTAASPHSSAAPAAGALPRRELDMLVSSGERVSMALLSIALHVRGVRA
                     VSLTGSQAGIVTSTLHFDARIQEIRRERLLNELATHDVVVVAGYQGVSPEREITTLGR
                     GGSDTTAVALAAAFGAARCEIYSDVDGVYTADPNRIQNARHLPELDYVTLQEMADAGA
                     KVLNARAVDWGR"
BASE COUNT         1284 a         2516 c         2456 g         1163 t
ORIGIN      
        1 ggcgcgaggg caacagctac gtcgaggtgc cggtcgccgc cgatggccgc gccgacgccg
       61 atcgccttgg cgaggcggcg caggacgcgg ccgccgtgct cgtcgggtat ccgagcttct
      121 acgggccgct ctccgatttg cggcgcgtcg cgagcgtcgc gcacgaccgc ggggcgttgc
      181 tcgtagcggt caacgccgag ccctacgcgc tcgcgctcgc cgagtcaccg ggcgcactcg
      241 gagccgacat cgcggttgga gaggggcagc ccatcgcgtg cccgccacag cttggcggcc
      301 ctggcgtcgg cctcttcgct tgccgtgacg accgccaatt cttgcagcag atgcccggac
      361 gcatcgccgg cgaaacggtc gacgtgaacg ggcagcgcgg ttacgtgctc acgctttcga
      421 cgcgcgagca gcacatccgt cgcgagcgcg cgacgagcaa tatctgcacg aaccaggggc
      481 tcatcgccct ctcgctcacg attcggatga gcttgctcgg aaagcgcggc ttcgtggagg
      541 tcgccgagca atgcctcgcc aaggcgcggt acctgagaag cgcgatcttg gctctccccg
      601 gctacgcgcc tggtttcggc gacgcgccgt tcttcaacga gttcgccatc aaggtgcgcg
      661 gtggcagcgc ggcgcgagtc atcgagcggc tcgaaagcga aggcatcgtc gccgggtacg
      721 acctcggaca ggtcgacccc gcctttgcgg atcgcttgct cgtcgccgtc accgagacgc
      781 acgagcgcgc ggatctggat cgcctcgttc ggggactcga gcgctgctga agcgctgcac
      841 ggtgagcgcg tctcttaggc gcgctcccgt gcccatgcgg cggtgctagc gcggaccgcg
      901 gccgcggtcg tcagtcgcga acgggttcga aaaccaattg gtgcccgtgg tgcggtacac
      961 cgtgaggcgg gcgaagtcga agccgagcgt gaacgaagcg aagcgctcgt cgtgcgtacc
     1021 catgccaccg gtaacgctca gggtaatgcc ccagggcgca ccgaggttga gcgcagcgag
     1081 cccgccttcg gagtggcggc cccaaaagcg ctcgtagccg ccggcgtcga gcgcgagacc
     1141 gaagccacct tggacgtccc cgcgcgtcgc aactcgcgcg agcagcgcga gatccgtacc
     1201 tgatttgaag tagggctgcg cccggaagac cccgcccacg accagcgtcg ctggcggcgt
     1261 gccgatccct gcgtcgagct gcaacgtgag ccgttggcga tcatcgcgga gaaacccggg
     1321 gccgagcccg gcataagccc aagaggacac gtctgcgcgc gcggacgatg gggcgaacga
     1381 gaacaacgca aaggcgacca agccccccgc gagggtgcta cgcgaagaaa acgacacggg
     1441 cgcgaggtac cacggaagag ccgtccccgg aagcccgccg tgcggccggt cgagcttcca
     1501 cgttgggcca atcacccgcc ctggcgcgct gatacgctct cgcgcgtgag ccttcgaaca
     1561 gttcgcgaga tcgattggga aaactggcgc ccgcgcgacg tcggaacgtt gctcttcatc
     1621 gtgcgcgacg gcgaagtgct cttgattcgc aagaagcgcg ggctcggcgc gggcaagatc
     1681 aatggccccg gcgggcgcct cgagccggga gagagccccc tcgcagcggc gattcgcgaa
     1741 gtcgaagaag aggtttgcgt gactccgtcc gaaatcgaag cgcgcggtga gctcagcttc
     1801 gacttcaccg acggctatcg cttgcacgcc cacgtgttcg tagcgttcgc gcacgcaggc
     1861 acaccgagcg cgacccccga ggccgaccct ttctggtgtt ctctcgagga gcttccctac
     1921 gccagcatgt gggcagacga tgcgctctgg ctacctcacg tgctcgccgg gcgcggcgtc
     1981 cgcgggcgct tcgtgttcga aggcgacgag atgctcgact acgagctcga cgtgttcgag
     2041 atcagctcac cggctcgccc gtgagcagca ccgcgaggtc cttcgcgccg agtacggcgt
     2101 agtttcgcac gcagttgttg aagatggcct gaacacgctc ggcttggctg gctagctgct
     2161 tcacgggctt cacccaagcc tcgagctcgg atggctcgta caggtaacca aagcgctcgt
     2221 ggacggtcgc acccttcttt ttccagccgc gctcgttctt cccatggaag cgaatgagag
     2281 cgagctccgg gtgggtcacc tcggcgacca gcggcatccc cggttcgtcg atcacgacga
     2341 agctcatctg ctcggctcga agcacgtcga agacgcgggc ccggcgctcc tccacgaacc
     2401 agcttttgtg ccgaaactcc accgcgaaag gcagcgcggg gtgcgccgcg cggatagcgg
     2461 agagcttttt cacgttgccg cgcgtcgccg tgaaccaagg cggaaactgc agcagcaccg
     2521 cacgcaaacg ctgggcgtcg ctgagcggcc gcaaaaaaga cagaaagcgc gcgtcgattt
     2581 ctccgagtag ctcgggcggc aacttgtcgg ggtaaacgcg gcgctcgtgc ccagcggcga
     2641 ccacggcttc tttcaggtcg gcgggcagcc gtgtgacgtc gatggggtga ccggtgagcg
     2701 aaggaaacgc tttgacgtcg aagctgaagt ctgccggcgt gaaacccacc cagcgggcgg
     2761 cggtatcggc gggcatcagc gtgtagtacg tcgcgtccac ctccacgaaa gggaagtgcg
     2821 ctgcgtagtg tcgcaggcgc gcttcgggtg tgctcgcgcc gcgcggatag aacgagtgac
     2881 tctcgatcag cgtgcgatcc gtccagctcg cagttccgag ttcgactcga cccacgcgag
     2941 ccggcagcgg cgcttgctcg gcgagcgact ctgcgcgcgc gaggcgctcg ggatcggcga
     3001 gcggcagcgc gagcttctct ttcttcggca cgcttcactc ctcggcggcc gaaagccagg
     3061 cgcgcagatg cttcgcttcg aaggggccga acacccgcag ccgctctcgt cctcgtcgat
     3121 cgagcaccac gagggtcggc actcgatcga tgttcgcaaa cgcgggaaaa ctcctgagct
     3181 cgacgcggtc ggcgatcgcg accgggtaac gcagatcgag cgtgtcgcgg aacgtctcga
     3241 ccaacacggc gttctccgcg gcctcgagcg caatgcacgc cgcgttgaag cgcggctttc
     3301 gcacccgata cacctcatcg agacgccgcg ccacgagctg actcgcgagg tcgtagctcg
     3361 tcgcgaagag caacgcagtg gctcgcccgc gagtgttctc cgcagtaaac accgtgccgt
     3421 cgagcacgcc gaatgcgaac tccggcggct ctcccactgg agggtgcacg gccacagggc
     3481 cctcaccgcg tgcaccaccg cagcccagta ggagcgcgac gcacgccacc gtaagcgctc
     3541 gacgtctcat cccaagatca ggtgtcgggc ggcggcggtg tggagccgcc cggcgctgga
     3601 ccgaaccaat ccgcgaatac tcgcagcgcc tctggcagat ctcgcccgct gcgtcggtgg
     3661 gccagcaccg cgtgcgccaa gtcatcgatg acctgcgtga gcggggtact ttcgagcttg
     3721 atgccttcga tgctttcgcc gtgcgccggc gcacgccgcc tctcgacgat gggatcgtgg
     3781 cgcacccaat cgaagcccat gtcggtgcgg tagccgcacg ccagcacctg cgcaaaatcg
     3841 aggtagagcg gagtgaccag caccgcgaca cgcatccccg tcgccgacac gccttggaac
     3901 gcctgagccg ccacgctggg cgtcgccgac tccatcggtc cgaccgcacg ctggatgcgg
     3961 gagcgacgct cgggatccga ctccaccgcg agcagcggcg cataaacagc gaacttcggg
     4021 accgagatca ggtccgcgtc ttgctcgagc gccttcagcc agttgtcccg cgcttccccg
     4081 ccgagctcgc gatacgcgat cgctgcggcg agcgccgcct cggcgtcggt ggccaaggcg
     4141 cgcagccact cacgccacgc atcgaccaaa cgcgactctc gctgcgcgcc cgtcagcggc
     4201 agcggcgtga gctcggcgag gttggcagag gtcggagtgc gacgctcggt ggattcactc
     4261 atggcttcga aaaaagccga gcggtgggcc gctcggaggt cgaaagtaag accggatccc
     4321 tctccgcggc aacccgaacg tcgccatcgg gagaagctcg tcgagattcc gacactttct
     4381 cgacctgact gccggggccg gcaatgccct cgcgcgggtc gggctcgacc acaccgtcga
     4441 gctcgagcgc caaatcgtgc acgatcgcca cgagtgcggc ttcgagtgag cgcggcgccg
     4501 cgacgaccaa atcgccccct tgctctgcga agagcacgcc aacgccctca ctcgcctcca
     4561 gcaccccttt gacgaacacg gcatcgcggg cgcgcacgcg cagacggcgg gcgaccaaat
     4621 cggcatcgag cagcgggggg acaggcacgg cggacggact agcacacaaa ggtcaccgag
     4681 cagccccggc acagtcttcg gggtcggccc cgataaggcc actctgctaa gctcattctc
     4741 cgatgaaaga gagctccgac gaccttgctc caggcagcct ccctccgctc gacaagagcg
     4801 aggacgcgga gctcgacgat gcggcgctcg gcggcctgct cgatccgctg cccacgctcg
     4861 atggcagcag cgacgacggc gacgcatcgg cgctcgatac gggcgtcgag acattcgaca
     4921 tcctcgccga caacgtcgag gacgccgcca cgaccatcga ctttggcggc aacttcgcgg
     4981 accttctggt cttgccttcg gagaacgaag agagcgacga cagcgtcggt ctcgcggcgg
     5041 cagatcccga tttcgtcaac gcccccgagg tgccagagcc ccgcgacgac gaagagggcc
     5101 tcgatgaacc agggctcggc ctcggctcgc tccccgagct cgtccaaggc gaagacgacg
     5161 agggcgtgga cgatacgggc gagttgccgg cttcggcacg cttcggcgac gagccgcgcc
     5221 cagcggtcgc agcaagggcg tgggccctcg cggggccgga gctgaagctc gagccgtgcg
     5281 ccgcgatcgc gagcatcgat ggcatcgtgg tcgccgcaag ctcggatcta ttttggttca
     5341 gtccggggaa cctcactccg gtgcgactcg aagccgggtc ctcacacgtc cacagcattg
     5401 ctctactggg cggcggttgg gactacgcgg cgtgcgctac cgcgagcgga aagctcattc
     5461 gtcgcggccg cctcgcttcc gcgtcagaag agctccggcg cgtgcgcgaa gagctcggct
     5521 cgcctggccg cgaagttttc gatctctgtc agcccggtcc ggcgttcccg cacaccctct
     5581 tgctcagaac cgcgagcgga aagcttctgc gcagcgacga cgacggcgtc acgtttcgac
     5641 gcgtgagcga acacaaggtc gtcgcgctcg cgccgcatgg cccgcccgcg ctcgccctct
     5701 cggccgaagg agtgctgttg tcgagcgacg acggcgccgg gagcttccgc gaggtgaagc
     5761 ttcaagggct cgccgcacgc gtcgcgctcg ccaagagccc actcatcgcc ggcaacgcga
     5821 aggcggtcct actgggcgat agcagcctcg gcgtgctttt gtcgaaagat cgcagcgagc
     5881 atttcgtccg cctcccggga acgctcggag tgagcgcgct ctgcatgggc ggcgacgccg
     5941 agtcaccttg tggtttcgcc gccgtctacg acgatgcgcg cgaccacacg ctgctcctgc
     6001 ggatcgatct cgcgacgttc ggcgtgagta cgatcgcgag catcgatgcg gactcgagcg
     6061 acgaggacga gatgaccgaa ggcgcgcgcg tcgccgcgct cgtgtgggac aatgtctacg
     6121 ggcggctgtg ggcggcgggc ggcttcggcg tcaaagctta cgctcccacc gacggctgat
     6181 agccgcgctc aacgacactt cttcgcgaaa gcatcttgct cgtcgagggc ggccttgagt
     6241 gaaagccgaa agccctcgac gttggcgggc gcgtcgcttt gcagcccggc aacccgatcc
     6301 gctatttctt ttcgcgcctt gtctgcgctg tcccacgtca cttgcgtggt catgaacgag
     6361 ctctcgagca actgcgccct tccctgcacg aacttccaac cctcgatgtc gactccaccg
     6421 agctgacggc gggctgcgga ctccgccaag ccgtgccagc tcttggcgag cgcggtacgc
     6481 gctttgtcac actcggcgtt gtccttgcag ctcagcgccc cgaacgcgaa tacgctcgcg
     6541 attacgagtt tcggaaacat ggaagaagcc ctttggtttc gacgatagct tccgcccgag
     6601 cggacgccaa gcaaaaccgc agggaattgg gcccaatcga gcggcgtttg atacttgtac
     6661 gctcaccggt cttcagaatt cggatcctcg ccggcgagca cttccctgct cgctcgcaga
     6721 aggtacgcac atggccatcg tcgttcagaa attcggcggg tcatctctcg cagacgtcga
     6781 gcgcgtcggg cacgtcgctg acttcgtgaa gaggacgagc caaagcgggt cgaagctcat
     6841 cgtggtcgtg agcgcgatgg gcaaaaccac tgaaaacctg ctcgatttgg cgcgaaccgc
     6901 ggcgagcccc cactcgagcg cggcccccgc tgccggcgcg ctgcctcgcc gtgagctcga
     6961 tatgctcgtc agcagcgggg agagagtgag catggccctg ctctcgatcg ccctgcacgt
     7021 gcgcggcgtg cgcgcggtga gcctcaccgg gtcgcaggcg ggcatagtca cgagcacact
     7081 gcattttgat gcccgcattc aagagatccg ccgtgagcgg ctcctgaacg aactcgcgac
     7141 tcacgacgtc gtggtcgtcg cgggatatca gggggtgagc cccgagcgcg aaatcacgac
     7201 cctggggcgc ggcggctccg acactaccgc cgtcgccctc gcggcggcgt tcggcgccgc
     7261 acgctgcgag atctacagcg acgtcgacgg cgtctacacg gcggatccga atcgcatcca
     7321 gaacgcgcgt cacctgcccg agctcgacta cgttacgctg caagagatgg cggacgcagg
     7381 cgcgaaagtg ctgaacgcgc gcgcggtcga ctggggacg
//
LOCUS       DASTKS010000635         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1887839, whole genome shotgun sequence.
ACCESSION   DASTKS010000635 DASTKS010000000
VERSION     DASTKS010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887839"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFQ35_13120"
     CDS             complement(<1..242)
                     /locus_tag="VFQ35_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931632.1"
                     /translation="MRSIAWLSFLLVCAAGCGRPATRAECDEIVGRIAELELRARGMT
                     GDNSDEVKATKDALEKTTLHDCVGRRLSDKAMACVRG"
     gene            391..1155
                     /locus_tag="VFQ35_13125"
     CDS             391..1155
                     /locus_tag="VFQ35_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931633.1"
                     /translation="MTRARTAVAESIAPEVFLRRATLARTSGARAKYAQRGLSHGGAL
                     DETTRSMLLRQLYLSHMEGREFEQALAVAVQMIDTAVMPDVARQDAARACLGLGDLDG
                     AIQHLRIAGRVSPAARRAFHLWTLGTVLYLNGRPEDAIGALERALRWGTIDKPLYAAQ
                     VALARHALGETDAEWESLREALADAPCGQGYGQFVLGELAFVQGDYTAAHRYLSAFVT
                     RSTGGRVALAVALEGEIRRARRLLRLLRGKRQRVQR"
     gene            1241..>2056
                     /locus_tag="VFQ35_13130"
     CDS             1241..>2056
                     /locus_tag="VFQ35_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9931634.1"
                     /translation="MHSFLRRLALLLPALLVGLLVAPSAWASAPDAFTRALEKGPIYA
                     GLAALLGGFLVSLTPCVYPMIAVTVSVFGARKAKSRWEGVGLSAAFVLGIVAMFVPLG
                     VIAGLSGSMFGSVLQSRWVLVGISLLFLAMASSLFGAFEFALPAGLTNRLAQMGGIGY
                     RGAFALGLACGLIASPCTGPVLTGILAFITQTRSAALGALAMGAFALGLGAPFFVVGA
                     FAVQLPKSGHWMVRIKSLLGIVLVVVAAYFLSTAFPGLGKWVKATWPFYAAASF"
BASE COUNT          277 a          696 c          683 g          400 t
ORIGIN      
        1 ccccgaacgc aagccatcgc cttgtcactc agccgcctgc ccacacaatc gtgcaaggtg
       61 gtcttctcga gcgcatcttt cgtggccttc acttcgtcgc tgttgtcgcc cgtcattccg
      121 cgggcgcgca gctcgagctc ggcaatgcgc ccgacgatct cgtcgcactc cgcgcgcgtc
      181 gccgggcgcc cacatcccgc cgcgcacacc aacaagaagc tcagccacgc gatcgatcgc
      241 atgcgggctg tttagcatcg cgagggcctt ccgcgctgcg gaacgggcgc tccgagcgac
      301 gcgtcgcggc agcggcgaga ggccaatgcg gtcggctatc cgatttgcag aaatctggac
      361 gtgtgtacag gtcttgctaa cctaacggac atgacacggg ctcgaactgc cgtcgcagag
      421 tcgattgctc ctgaggtgtt tttgcgacgt gcgaccctcg ctcgaacttc cggcgcacgg
      481 gccaaatatg ctcagcgcgg cctctctcac ggaggcgcgc tggacgagac cacgcgctcg
      541 atgctgctgc ggcagctcta cctctctcat atggaggggc gggagttcga acaggcgctc
      601 gccgtagccg tgcagatgat cgacacggcc gtgatgccag acgtcgctcg ccaagacgct
      661 gcacgggcgt gcctcgggct cggcgatctc gacggagcga ttcagcacct ccgcatcgcc
      721 ggacgcgtga gcccggctgc gcgacgagcc ttccatctgt ggacgctcgg caccgtgctc
      781 tacctcaatg ggcggcccga ggacgccatc ggcgcgctcg agcgagcgct ccgctggggc
      841 accatcgaca agccgctcta cgcagctcag gtcgcattgg cgcggcacgc gctgggagaa
      901 accgacgcag agtgggagtc gctgcgcgag gcgctcgccg acgccccttg cggtcagggc
      961 tatggtcagt tcgtcctggg ggagctcgcg ttcgtccaag gcgactacac cgcggcacac
     1021 cggtatctct ccgccttcgt cacccgttct accggaggcc gtgtcgcgct cgcggtcgcg
     1081 ctcgagggag aaatccgtcg tgctcgtcgg ttgttacggc tcctgcgtgg caaacggcag
     1141 cgagttcaga ggtagcgccg cttcgacatc catcgcgcgg agcagggtgc gcgtctcgac
     1201 cttgccccac ctgccgcgcc gtgtgacacg cttggggtcg atgcactcgt tcctccgccg
     1261 tttagccctg ctgctccccg cgctgctcgt cgggttgctc gtcgcgccga gcgcgtgggc
     1321 gagcgcacct gacgcattca cccgcgccct cgagaagggg ccgatttacg cagggctcgc
     1381 ggcgctactc ggcggcttcc tcgttagcct gactccctgc gtgtacccga tgatcgcggt
     1441 gacggtgagc gtgttcggcg ctcgcaaagc gaagagccgc tgggaaggcg tcgggctctc
     1501 ggcggccttc gtgctcggca tcgtcgcgat gttcgtgccg ctcggagtca tcgcgggcct
     1561 ttctgggtcc atgttcggct cggtgctgca gagccgctgg gtgctcgtcg gcatctcgct
     1621 cctgttcttg gcgatggcct cgtcgttgtt cggcgccttc gagttcgcgt tgccggcggg
     1681 gctcacgaat cggctcgcgc aaatgggtgg catcggctac cgcggtgcat tcgcgctcgg
     1741 cctggcgtgc gggctcatcg catcgccgtg caccggcccg gtgctgacgg ggatcctggc
     1801 tttcatcact cagactcgta gtgcggccct cggggcgctc gcgatgggag ccttcgcgct
     1861 cgggctcggg gcgcccttct tcgtggtggg tgcgttcgcc gtgcagctac cgaagagcgg
     1921 ccattggatg gttcgcatca agtcgctgct cggcatcgtg ctcgtcgtcg tggcggcgta
     1981 ttttctctcg acggcgttcc cagggcttgg caagtgggtg aaggcgacct ggcccttcta
     2041 cgccgcagca tcgttc
//
LOCUS       DASTKS010000636         4194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_548435, whole genome shotgun sequence.
ACCESSION   DASTKS010000636 DASTKS010000000
VERSION     DASTKS010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548435"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFQ35_13135"
     CDS             complement(<1..723)
                     /locus_tag="VFQ35_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9931635.1"
                     /translation="MIDSNPSAAAPFPSSADELAPSPQRQSVFRTLKDALRGVETDLT
                     EAPLGRALVMLAIPMILEMIMESIFAMVDVFWVAHLGAEAVATVGLTESMMMIVYSLA
                     MGTAIGTMALVARRVGEKDFELAARTAVQGIGLAAVFAIALGVVGAAFAPNLLRVMGA
                     DDAVVETGTRFTRIMLGGNLTVFLLFMINSVFRGAGDAALAMRVLWLGNALNIVLGPL
                     FIFGVGPFPRLGVAGAAVATTLG"
     gene            complement(820..1632)
                     /locus_tag="VFQ35_13140"
     CDS             complement(820..1632)
                     /locus_tag="VFQ35_13140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9931636.1"
                     /translation="MTDFGRIADQIEADAWTALFDAMPSPMKSALGARVERIGGVTVL
                     LAPGIPLSYFNRAIGLGMSSEVQEEDVGSLAELFEHAGCPASNIHLAPYARAHGDIRG
                     WLAAAGYALLPTRPTWGKFLRGPEACPVIQTELEVEPAEARDAAAVAAVLCDAHGMPP
                     SIAPWTAALVASPRLRTYRVLDGDAVVGGALLYSSGNRAWLGLGGVLPSHRRRGAQKA
                     LMRRRIVDAARLGAEHLATETGEPLGEERNPSFSNMLAMGFRKVMSRENWTR"
     gene            complement(1629..2384)
                     /locus_tag="VFQ35_13145"
     CDS             complement(1629..2384)
                     /locus_tag="VFQ35_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9931637.1"
                     /translation="MTLDDALGLAIPITYLTFLGVEARWPARSFPRVRGWRLIGWLAL
                     MMLMTINVALPLALPEPWLRSHRLLDGTRLGVVGGIVVGYAAVTFATYWWHRLEHRSD
                     WVFRFIHQLHHSPVRMDVSGGAYLHPLDIGASIVLSLLVTVFVLGLDPVAAAIVGYVG
                     AFYAMFQHLNVKTPPWLGYLIQRPESHCLHHERGVHARNYSDLPLWDIVFGTFHNPRS
                     FAGEVGFGGPAPRRIVAMLLGVDVDRDAKRSEE"
     gene            2437..3189
                     /locus_tag="VFQ35_13150"
     CDS             2437..3189
                     /locus_tag="VFQ35_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9931638.1"
                     /translation="MKRKAGLAPLKTATAPKTRLGRPLLSRDVIAERALEVIDAHGLE
                     ELSTRKLARTLGVEAMTLYHHFPTKAALLDAVAEKLTEMIAPPKLGTDALDTFISGAH
                     GLYAVAVEHPRAFSLLATRRANTPAMLAFYDALLAPLLAAGYSPKDAALIFRATGYFT
                     LGAGLARVATFAEEIEGARPLPESAEALASYPSLSVVAPHLTRARVDALFDRGLCALS
                     AGLRAGLTVAGEAPNEPRGGGHRGGGGKKNRK"
     gene            3329..>4194
                     /locus_tag="VFQ35_13155"
     CDS             3329..>4194
                     /locus_tag="VFQ35_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HET9931639.1"
                     /translation="MSIGLRRLRTWFGLCSLGAALWVSACSGKKDMDADASGGTDSGG
                     STSAGGVTNKGGVTGKGGATAEGGETTGGTLTSGGAVSEAGAGAAPAEGGAGGVLGVG
                     GAPELGDGSGLPNAPAGSWTYLVYMLADNNLEPFGLDDMKELMSVGSGGNVTILAEID
                     RAVGESSAPIGGLPNFTSTKRVRVDAGKLTELEDLGEQNLGSAQTFQAFLEWGLTASP
                     SEHYAVILWDHGGAWPRYGADDSAQGDGLTLPELTRGIDAAAKSTGLLGPIDVLGFDA
                     CLMGTWEVAASFA"
BASE COUNT          799 a         1384 c         1373 g          638 t
ORIGIN      
        1 accgagagtc gtcgctaccg ccgcgcccgc aacgcctagg cgaggaaacg gcccaacgcc
       61 gaagatgaag agcgggccaa gcacgatgtt gagcgcgttg ccgagccaaa gcacgcgcat
      121 ggccaacgcg gcgtcgcccg cacctcggaa caccgagttg atcatgaaca gcaagaacac
      181 ggtgaggttt ccgcccaaca tgatgcgggt aaagcgtgtg ccggtttcca cgaccgcgtc
      241 atcggcgccc atcacgcgca gcaggttggg cgcgaacgcg gccccgacga cgccgagcgc
      301 gatcgcgaac acggccgcga gcccgattcc ctgcacggcc gtgcgcgcgg cgagttcgaa
      361 gtccttctcc ccgacgcggc gcgcgacgag tgccatcgtt cctattgccg tgcccatggc
      421 cagcgaatag acgatcatca tcatcgattc ggtgaggccc accgtcgcga cggcctccgc
      481 gcccaggtga gccacccaga acacgtcgac catcgcgaag atggactcca tgatcatctc
      541 gaggatcatg gggatggcga gcatcacgag cgcgcggccg agcggagcct cggtgaggtc
      601 cgtttcaacg ccgcggagtg cgtccttcag cgtgcgaaac acgctctgac gttgcggcga
      661 aggcgcaagc tcatccgctg aactcgggaa aggggcagcc gcacttgggt tcgaatcgat
      721 catggctcgc gaagacgacg aacgatgacc ccgcagatcg acacgtcgag gcgagtttct
      781 gaaattcgga cgcaagcact cgaaacaact gaatttttct cagcgcgtcc agttctctcg
      841 cgacatcacc ttgcgaaatc ccatcgcgag catgttggaa aatgagggat ttcgctcttc
      901 gcccaatggt tcgcccgtct ccgtggctag gtgctcggcc ccgagtcgcg ccgcgtcgac
      961 gatgcgacga cgcatgagcg ctttctgagc gccgcgccgc cggtgcgagg ggagcacccc
     1021 gccgaggccg agccatgccc gattgccgct cgagtagagg agagccccgc cgacgaccgc
     1081 atcgccgtcc agcacgcgat aggttcgcag ccgagggctc gcgaccagag ccgccgtcca
     1141 gggtgcgatc gagggcggca tcccgtgcgc atcgcagagc accgccgcga ccgcggctgc
     1201 gtcccgcgct tcggcaggtt cgacctcgag ctccgtctgg atgactgggc atgcttccgg
     1261 accgcgcaag aactttcccc atgtcggtcg agtcggcagc aatgcgtacc ccgctgcggc
     1321 caaccaaccc cgaatgtctc catgcgcgcg agcgtaaggc gcgaggtgga tgttggacgc
     1381 aggacaaccc gcgtgttcga agagctcggc gagcgaccca acgtcctcct cctgaacctc
     1441 ggaactcatg ccgagaccga tggcgcggtt gaagtacgaa agtgggatcc caggagccaa
     1501 gagaaccgtc acgcctccga tgcgctccac gcgggcgccc agcgcgctct tcatggggct
     1561 cggcatggca tcgaacagcg ccgtccaagc gtcggcttcg atctgatcgg cgatgcggcc
     1621 aaagtcggtc attcttcgct cctcttcgca tcgcgatcga cgtcgacgcc aagcagcatg
     1681 gcgacgatgc gccgcggcgc ggggccgccg aagccgacct ctcccgcgaa gctccggggg
     1741 ttatggaagg tgccgaacac gatatcccag agcggcaagt cgctgtagtt gcgggcgtgc
     1801 accccgcgct catgatgcag gcaatgcgac tcgggccgct gaatcaggta gccgagccag
     1861 ggtggtgttt tcacgttcag gtgctggaac atcgcgtaaa aggcgccgac ataacccacg
     1921 atcgcggccg cgacgggatc caagcccaac acgaacaccg ttacgaggag agagagcacg
     1981 atgctcgcgc caatgtccag agggtgcaga taggcacctc ccgacacatc catgcgcacc
     2041 gggctgtggt ggagctggtg aatgaaacga aacacccagt cacttcggtg ctccaatcgg
     2101 tgccaccaat aagtggcgaa ggtgaccgca gcgtagccga cgacgatgcc gccaacgacg
     2161 ccgagccgcg tgccatcgag caaacgatgc gagcgcagcc aaggctcggg tagcgcgagc
     2221 gggagcgcga cgttgatggt catcaacatc atcagcgcaa gccagccaat gagcctccaa
     2281 ccgcgcaccc gcgggaacga gcgcgccggc caccgcgcct ccacgcccag aaaggtcagg
     2341 taggtgattg gaattgcaag ccccagagcg tcgtcgagag tcacgcgagt ctcctttgac
     2401 ttcccttacg atgcaaggga agattcggat caccgcatga agaggaaggc ggggctcgca
     2461 cccctgaaga cagcgaccgc acccaagacg cgccttgggc gcccactgct cagccgcgac
     2521 gtcatcgccg agcgcgcgct cgaggtgatc gatgcccatg ggctcgagga gctctcgact
     2581 cgaaagctgg cgcgcacgct gggagtcgag gcgatgaccc tctatcacca cttcccgacc
     2641 aaggccgcgc tcttggacgc ggtcgccgag aagctcacgg agatgatcgc gccgccaaag
     2701 ctcggcaccg acgcgctcga taccttcatc agcggagcgc acgggctcta cgccgtcgcc
     2761 gtcgagcacc cgcgcgcgtt ctcgctgctg gccacgcgcc gcgccaacac tcccgccatg
     2821 ctggccttct acgacgcgct cttggctccg ctcctcgcgg cgggctactc gccgaaagac
     2881 gcggcgctga ttttccgtgc caccgggtac ttcacgctgg gcgcagggct cgctcgagtc
     2941 gcgacgttcg ccgaggaaat cgagggagct cgcccgctgc cggaatcggc cgaggcgctc
     3001 gcgtcgtacc cgagtctaag cgtcgtcgcg ccgcatttga cccgcgcgcg cgtcgacgcc
     3061 ttgttcgacc gcgggctgtg cgcgttgtcc gcagggctcc gcgccgggct aacggtcgcg
     3121 ggcgaagcgc ccaacgagcc tcgaggcggc ggccatcgag ggggcggcgg caagaagaac
     3181 cgcaagtagc cgggccagcg gctctttcga ccccggctct tacactcaac gtgaaacagc
     3241 gatgtcaacg agccgctccg tgagcttcgg tataggcgag ctgtcgcagc cggtgtaagt
     3301 tcgcccgatc gtaaaagagg caatggaaat gagcatcgga ttgcgacggc tccgcacttg
     3361 gtttggcctg tgctccctcg gcgcggctct ttgggtttcg gcctgctcgg gcaagaagga
     3421 tatggacgcc gacgcgagcg gagggaccga cagcggcggc tcaacgagcg ccggcggagt
     3481 tactaataaa ggcggagtaa ccggtaaggg cggagcgact gccgaaggcg gtgaaacgac
     3541 cggcgggacg ctgacgagcg gcggggccgt atccgaggca ggcgctgggg ccgcacccgc
     3601 tgaaggcggc gccggtggcg tcctgggtgt cggcggcgcg cctgagctag gcgacggcag
     3661 cggcttgccg aacgcacccg cagggagctg gacctacctc gtgtacatgc tcgccgacaa
     3721 caacctcgaa ccgtttggcc tcgacgacat gaaggagctc atgtccgtgg gctcgggcgg
     3781 aaacgtgacc atcctggcag agatcgatcg cgcagtcggg gaatcctcgg cgccgatagg
     3841 cgggctgccg aatttcactt cgacgaagcg cgtccgagtc gacgccggca agctcaccga
     3901 gctcgaggac ttgggagagc agaatctcgg cagcgctcag accttccaag cgttcctcga
     3961 atggggcctc accgcctccc cttcggagca ctacgcggtc atcctttggg atcacggcgg
     4021 cgcttggcct cgctacggcg cggacgactc ggcgcaagga gacggcctca cgctgccaga
     4081 gctcacccgt ggcatcgacg ccgcggcgaa gtcgaccggg ctgctcggtc cgatcgacgt
     4141 gttggggttc gacgcttgct tgatgggaac ctgggaggtt gccgcatcct tcgc
//
LOCUS       DASTKS010000637         5640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1441716, whole genome shotgun sequence.
ACCESSION   DASTKS010000637 DASTKS010000000
VERSION     DASTKS010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441716"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFQ35_13160"
     CDS             <1..491
                     /locus_tag="VFQ35_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931640.1"
                     /translation="TSFYENVPQNTDTIVAVAEAKYFVIPPPSLDAQGSASTGLSTIA
                     IGFTRESSNSYLASYYERTGGYASLVYFQGGAFVAQLDGGYYHYYFPESRAFHTFTQD
                     RLNARFFAEYRFSNSLGLNGTVAYDQNLSGNLRPQTGSTVPIDNLDFNRWQAFIGLRW
                     FM"
     gene            455..1120
                     /locus_tag="VFQ35_13165"
     CDS             455..1120
                     /locus_tag="VFQ35_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9931641.1"
                     /translation="MASIHRPALVHVNGSCTPGVRRLDFVLWMLSAPAALLVASCGGG
                     NAAQHVNLPAPSERTLVGAGDVLTIEIVGEKELPREYQVASDGSIDVPYVHAVKVGGL
                     DPHQVAELVRKALIEAKVLTDPSVIVSVKEYHSQRVTLLGQVAKPGSYPFSAGLTLIQ
                     AVSLAGGLTAIADDDKVTITRKADNGKTYTAVLSVAPIMVGRAADIPLQAGDQIYVRE
                     RLF"
     gene            complement(1247..2257)
                     /locus_tag="VFQ35_13170"
     CDS             complement(1247..2257)
                     /locus_tag="VFQ35_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931642.1"
                     /translation="MRRSPLRRLAGLAALTSALSATFAANASDCRPGNGVSPCLDANA
                     LWLTTGDARFFSLPSGRVLGAGHVGLAFAASAAWRPLWLNVPSSNADGRDVELVERRV
                     EEETLLAVGLGKSLELGLALPVVLHQSGSGSQGITSQNGAALDGTGERDPRVGLSLGL
                     HFGALRLKPRIGVALPLGSTDAYASAGAFTFAPAVPLAFRHGRFEHAVELGLRLRPSV
                     EIGGVRVGSEATLAGGSSYAILRNELLVLCAEIFFLPSLVDNQAAKAKSFEANSTLLA
                     AEYLISVRSRPSVHDPVTIALGAGSALALSRQSSPAGDERFVAPGGPAFRLLAEVRYA
                     PE"
     gene            2359..2967
                     /locus_tag="VFQ35_13175"
     CDS             2359..2967
                     /locus_tag="VFQ35_13175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HET9931643.1"
                     /translation="MIDQARPLLLGSASPRRREILEGLRIPISVCPADIVEDVLPGEA
                     PQDYLARIVREKLGAVAARAAGLAYGALLVADTSVVLGREILGKPADVADAERLLASL
                     SGRTHIVYTRYAIARAEAPSVPVAERTVESEVTMRAASAEELRRYAATGEGLDKAGAY
                     AVQGIGSFLVESIQGSYSNVVGLPACEVVRDLLELGVLSQFP"
     gene            2964..4409
                     /locus_tag="VFQ35_13180"
     CDS             2964..4409
                     /locus_tag="VFQ35_13180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9931644.1"
                     /translation="MKSGDASRFWRAEPRRVDEVVTVALSLVVRAAVVAWAHGRFPPA
                     DDGKFYHVVADRIAHGLGYTWQWPDGVVTYAAHYPVGYPALMGAGYAVFGSSPTVAMA
                     LNAFIGTLGVWAVYRLASFNRKRGPAVVAGVVAALHPSLVFYTPALMTEGVTAALLAV
                     LGWLAVTARARGIGWLVALGLLSGVLVLVRPQALVLAPVLGAVAGVDGWPSRLRRAAL
                     VTVLALGVCLPWTLRNCSRLDHCAFVSANGGWNLFIGSAEKATGRWVSIDELGVPDEC
                     RNEFGEAGKDRCFGRAGTRNIREHPVHFLKLIPAKLSATFDWSGAPGHYLHSSNGALV
                     DEHGKLQLGIAEALYQRIVLLLGFVGVGLAPGPRRGARMLAAALSAVWLFRPEAWLSH
                     LLLVLLVASFGRSLWSRPAWLLGAAAVLATAVTHAVFFGAGRYGLVCVLLLVAFAAEG
                     LAALGGGKRPFVEARRLAPSLLSFDSRSSGE"
     gene            4420..4683
                     /locus_tag="VFQ35_13185"
     CDS             4420..4683
                     /locus_tag="VFQ35_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931645.1"
                     /translation="MPLIETEEAARRLARAIASDLSLYNEEKIIRGIEGDTLFDELAE
                     EIEEGRALYKSRVSPDLYHRNYYDRALVDILVKSKGHIKSKIW"
     gene            4680..5636
                     /locus_tag="VFQ35_13190"
     CDS             4680..5636
                     /locus_tag="VFQ35_13190"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9931646.1"
                     /translation="MSRLRFVLGPGERERIDKVLTRLWQGASRATVQRLIEQGKVWVN
                     GAPCRARDSVGEGSVIELEPQPPEPSAATPDAGVRFDVVHEDDDVLVVDKPAGLVVHP
                     ARGHRQGTLVNGLLARDSFTASDFEDDSDVHVRPGIVHRIDKDTSGLLVIAKNEAARE
                     GLKAQLAAHSMERVYRAITQGVPRVTTIRSLHGRDPRSRLRFTSRVEEGKHAVTHVRV
                     VEVLANAKAALVECRLETGRTHQIRMHLTEIAKTPLLADALYRTPNQPKELQPIVEGL
                     GRQALHAAVLGFEHPRTKLALRFESALPADMESALAALRALQ"
BASE COUNT          897 a         1844 c         1916 g          983 t
ORIGIN      
        1 aaacgagctt ctacgagaac gttccgcaaa acaccgatac aatcgttgcg gtcgccgaag
       61 cgaagtactt cgtcattccg ccgccgtcgc tcgacgcgca gggttcggcg tcgaccggtc
      121 tctcgacgat tgccatcggc tttacgcgcg aatcgagcaa cagctatctc gcgtcgtact
      181 acgagcgcac gggcggctac gcctcgctcg tgtacttcca gggcggcgcg ttcgtcgcgc
      241 agctggacgg cggctactac cactactact tcccggagag ccgcgcgttc cacacgttca
      301 cgcaagaccg cttgaacgcg cgttttttcg cggagtaccg cttcagcaac tctctcgggt
      361 tgaacggcac cgttgcctat gaccagaacc tgagcggtaa tttgcggccg cagacgggct
      421 cgaccgtccc gatcgacaat ctcgatttca atcgatggca agcattcatc ggcctgcgct
      481 ggttcatgtg aatggctcgt gcacgccggg cgtgcgcagg ctcgatttcg ttctctggat
      541 gctctcggcg ccggcggctt tgctcgtggc gtcgtgcgga ggcggcaacg ccgcccaaca
      601 cgtcaatttg cccgcgccgt cggagcggac gctcgtcgga gcgggcgacg tgctcacgat
      661 cgagatcgtc ggcgagaagg agttgccgcg cgagtaccag gtcgcttccg acggcagcat
      721 cgacgtgccg tacgtgcatg cggtgaaggt cggcggcctc gacccgcatc aggttgcgga
      781 gttggtgcgc aaagcgctga tcgaagcgaa ggtcctcacc gacccgagcg tcatcgtctc
      841 ggtgaaggag tatcacagcc agcgtgtcac gttgctcggg caggtcgcga agcccgggag
      901 ctacccgttt agcgcggggc tgacgttgat ccaggccgtc tcactggcgg gcggcctcac
      961 tgccatcgcg gacgacgaca aggtcacgat cacgcgcaag gcggacaacg gcaagacgta
     1021 tacggcggtg ctcagcgtcg caccgatcat ggtggggcgc gccgccgaca ttccgctgca
     1081 ggcgggcgac caaatctacg tgcgcgaacg gctcttctga tttcgctctg agagcgaacc
     1141 gaacgaacgc gagccgccgc gatttgacgg ctcgcggcgg cacctcgggc acgtgcgggc
     1201 catcgcgttc gcactcgacc cggctgcgtt atgaggagct gcgcgctcac tccggggcgt
     1261 agcgcacctc tgcgaggagc cggaacgctg gacccccggg cgcgacgaag cgttcgtcgc
     1321 cggcgggcga cgactggcgc gacagtgcga gcgcggatcc cgcgccgagc gcgatggtta
     1381 cgggatcgtg gacgctcgga cgcgagcgaa ccgaaatcag gtattccgcc gcgagcaggg
     1441 tcgagttcgc ctcgaagctc ttcgctttcg ctgcctggtt atcgacgagg cttggcagaa
     1501 agaatatctc ggcgcacagc acgaggagct cgttgcgcaa aatcgcgtag gacgagcccc
     1561 cggcgagagt tgcttcggag ccaactcgca cgcctccgat ttcgacgctc gggcgcaatc
     1621 gaaggccgag ctctactgcg tgctcgaacc ggccgtgtct gaatgcgaga ggtaccgccg
     1681 gcgcgaacgt gaaggctccc gcgctcgcgt aggcatcggt gctgccgagc ggcagcgcga
     1741 ctccaatccg cggcttcaac cggagcgctc cgaagtgcag cccaaggctc aagccaacgc
     1801 gcgggtcgcg ctcgccggtt ccgtcgaggg ccgcgccgtt ttgagaagtg attccctggc
     1861 tgccgctgcc agattggtga agcaccaccg gtagagcgag gccgagctcg aggctttttc
     1921 cgaggcccac cgcgagcagg gtttcttctt ccaccctacg ttcgacgagc tctacatcgc
     1981 gtccatcggc gttcgacgac ggcacattca gccagagcgg gcgccatgca gcgctcgcgg
     2041 cgaaggccaa cccgacgtgc ccggcaccga gcactctccc gctcggaagc gaaaagaagc
     2101 gcgcgtcgcc ggtggtgagc cacagggcgt tcgcgtcgag acaaggcgag acaccgttgc
     2161 cggggcgaca atcgctcgca ttggcagcaa aagtcgccga tagcgcgctc gtgagcgccg
     2221 cgagcccggc cagtcgacga agggggctcc gacgcatggt gccgcacgtt acacccaagg
     2281 gcccacgaat gggtgagcca tggcgtctgc gagaacggca gcattgacgc cccgaccaga
     2341 tccttgcatg gcccgggcgt gattgaccag gctcgtccct tgctcttggg ctctgcctcg
     2401 ccgcgccggc gcgaaatcct cgaagggctc cgcattccga tctcggtgtg cccagccgac
     2461 atcgtggagg acgtgcttcc tggtgaggcg ccgcaggact acctcgcgcg aatcgtgcgg
     2521 gagaagctcg gcgcggtggc ggcacgggcg gccgggctcg cgtacggcgc gctgctcgtc
     2581 gccgacacat cggtcgtcct cgggcgcgag attctgggca agccagcgga cgtggccgac
     2641 gccgagcgct tgctggcgag cctcagcggt cgcactcaca tcgtttacac gcgctacgcc
     2701 atcgcacgtg ccgaggcgcc cagcgtcccc gtcgcggagc gcactgtcga atccgaagtg
     2761 acgatgcgag ctgctagcgc cgaagagcta cgtcgctacg cggcgaccgg cgaggggctc
     2821 gacaaagcgg gcgcctacgc cgtccagggc atcggctcgt tcttggtcga gtcgatccag
     2881 ggctcgtact cgaacgtcgt cgggttgccg gcgtgcgaag tcgtgcgcga cctcttggag
     2941 ctcggcgtgc tctcgcagtt tccatgaaat cgggcgacgc gagccgcttt tggcgcgccg
     3001 agccgcgtcg tgtcgatgaa gtggtgacgg tggcgctttc gctcgtcgtg cgcgcggcgg
     3061 tggtcgcgtg ggcgcacggg cgttttcccc ccgccgatga cggcaagttc tatcacgtcg
     3121 tggcggaccg aatcgctcac ggtctgggct acacctggca atggccggac ggtgtggtga
     3181 cgtatgccgc gcattacccc gtgggctatc cggccctgat gggggcaggg tacgctgttt
     3241 ttggttcttc acccacggtg gcgatggcgc tcaacgcgtt catcgggacg ctgggggtct
     3301 gggcggtcta ccgcttggcg tcgttcaatc ggaaacgcgg tcctgccgtc gtggccggcg
     3361 tcgtcgcagc gttgcaccca agcctcgtct tttacacgcc ggcgctgatg accgaaggcg
     3421 tgacggcagc gctcctcgcg gtgctgggct ggctcgcggt caccgcacga gcccgtggca
     3481 tcggttggct ggtggcgctg ggcctgcttt cgggagtgct cgtactggtg cgaccgcaag
     3541 cgctagtgct cgcgccggtg ctcggcgcgg tcgcaggcgt tgacggctgg ccttcgcgcc
     3601 tgcgacgcgc tgcgctcgtc accgtgctcg cgctcggcgt ttgtctgccc tggacgttgc
     3661 gcaattgcag ccggctcgac cattgcgcct tcgtgagcgc caatggcggc tggaacctgt
     3721 tcattggctc cgcggaaaag gcgaccggcc gctgggtctc aatcgatgag ctcggcgttc
     3781 ccgacgagtg ccgcaacgag ttcggggaag ctggaaaaga tcgctgcttc ggtcgggcgg
     3841 ggacaagaaa catccgcgag caccccgtgc acttcctgaa gttgattccg gccaagctga
     3901 gcgcgacgtt cgactggtcc ggcgctcccg ggcactatct gcattcgtcg aacggggcgc
     3961 tcgtcgacga gcacggcaag ttgcagctcg ggatcgcgga ggcgctctat cagcgcatcg
     4021 tcctcttgct gggcttcgtt ggcgtggggc tcgcgccggg tccgcgtcgg ggcgcacgca
     4081 tgctcgcagc ggcgctttcg gccgtctggt tgtttcgccc ggaagcctgg ctctcacacc
     4141 tgctcttggt tttgctcgtc gcgagtttcg ggcgctcgct ctggtcgcgt ccagcctggc
     4201 ttctcggggc ggcggcggtc ctagccaccg ccgtcaccca cgccgtgttc ttcggcgcgg
     4261 ggcgatatgg gctcgtgtgt gtgctcctgc tcgtcgcatt cgcggcagaa gggctggcag
     4321 cgctcggcgg cgggaagcgg ccgttcgtcg aggcgaggcg gctcgcgccg tcgctcctct
     4381 cttttgacag ccgaagctcg ggcgagtgat acttccgcca tgccgctcat cgagaccgag
     4441 gaagccgccc gccgcctagc tcgcgcgatc gcgagcgatc tttcgctcta taacgaggag
     4501 aagatcatcc gcgggatcga gggcgacacc ttgttcgacg agctcgcgga agagatcgag
     4561 gaaggccgcg cgctctacaa gagccgcgtc tctcccgacc tctaccaccg caactactac
     4621 gatcgcgctc tcgtcgacat cctggtgaag tcgaaggggc acatcaagag taagatctgg
     4681 tgagtcggct gcgcttcgtg ctcggcccgg gggagcgcga gcgcatcgac aaagtgctca
     4741 ctcggttgtg gcagggcgct tcgcgcgcca cggtgcagcg cctcatcgag cagggcaagg
     4801 tctgggtgaa cggcgcgcca tgccgcgctc gcgacagcgt gggtgagggg agtgtcatcg
     4861 agctcgagcc tcagccgccc gagccgagcg cggccacgcc cgacgcgggt gtgcgtttcg
     4921 acgtcgtgca cgaggacgac gacgtgctcg tcgtcgacaa gcccgccggg ctcgtggtgc
     4981 atccggcgag agggcatcgc cagggcacgc tggtaaacgg acttctggcg cgcgattcgt
     5041 tcaccgcgag cgacttcgag gacgattcgg acgtccacgt tcgacccggg atcgtacacc
     5101 gcatcgacaa ggatacgagc ggcctgctcg tcatcgccaa gaacgaggcg gcgcgggagg
     5161 ggttgaaagc gcagctcgcg gcccacagca tggagcgcgt atatcgcgcg atcacccaag
     5221 gcgttccgcg cgtaacgacg atccgctcgc tgcacggtcg cgatccgcgt tctcggcttc
     5281 gcttcacctc gcgcgtcgaa gagggcaagc acgcggtcac gcacgtgcgc gtcgtggagg
     5341 tgctcgcgaa cgccaaggcc gcgctcgtcg agtgccgcct cgagaccggg cgcactcatc
     5401 agatccgcat gcacctcacc gagatcgcca aaaccccgct gctcgcggat gcgctgtacc
     5461 ggactccgaa tcaaccgaag gagcttcagc caatcgtcga agggctcggc cgccaagcgc
     5521 tgcacgcggc cgtcctcggc tttgagcacc cgcgcacgaa gctcgcgctg cgcttcgagt
     5581 cggcgttgcc cgccgacatg gaaagcgctc tcgcagcgct gcgcgccctg caatgagcgc
//
LOCUS       DASTKS010000638         6288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1217777, whole genome shotgun sequence.
ACCESSION   DASTKS010000638 DASTKS010000000
VERSION     DASTKS010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217777"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1448)
                     /locus_tag="VFQ35_13195"
     CDS             complement(150..1448)
                     /locus_tag="VFQ35_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931647.1"
                     /translation="MSDEPVRLAPRVEELLSALPTPERSDWEGLARQIDQRVRETEAK
                     STAPLWLMAPLPDEPTEPGAQAQTASDEPSSGAAPSADAQRSAPASSPPSAAAGPAPA
                     TSLAALARSVAQRDARPEAAHIAKESISLAAAARVHTDGIVERVRSARPSPTRDVDAR
                     VPPAPPSARVAPAVAPAATPPPARRSELGPWIAIGGMGAALAAALMVIAFRREPAQIV
                     VAAPPAATAAAPAAPVAQAQAPTQAPAPAQAVAPTEDAPRAEVASAPPTLAASSLPLA
                     SKNASPTSPAHANSEPVAVASRATTAVRPAPEKIQLEETPAKVKPASPTEPPAAGVSG
                     MRPAAGSTTSGGLPDRPSTGAVQAAVGSVMGAARACVAGGAATQAQITFGSDGTVSSV
                     AVNGPSAGTSAAKCIEAALKRARVAPFTNQSFSLGIWVRP"
     gene            complement(1441..2025)
                     /locus_tag="VFQ35_13200"
     CDS             complement(1441..2025)
                     /locus_tag="VFQ35_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931648.1"
                     /translation="MSERTDRELVEAAQKGDIEAFGVVVRRHQKRVYRLALHLLRSAA
                     EAEDVTQDTFVRAYGALDRFDGRSEPFTWIYRIAVNLSLNAIRARKHSERSSSPDDPR
                     VEPQLIEQRAKLASPESANSDRELAVALEAGIDSLSEVLRTTLLLVVVDGLPHADAGK
                     ILGCSEGTVAWRIHEARRRLREYLSQRGYGENHV"
     gene            complement(2237..3937)
                     /locus_tag="VFQ35_13205"
     CDS             complement(2237..3937)
                     /locus_tag="VFQ35_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9931649.1"
                     /translation="MSSEQAEKPEIANGDEALASVEATREAAVSDDAQASDGDADSDD
                     SADSSDEGESAAAEGAASASDPTAKKKRRRKKRKKKPEGAAQEAEARDAQRPHRASER
                     APFHVGEEVFGKVTAVLETAIMVDLSGKALAIFDRGEMEPDDLIPAVGDRLVARVHND
                     GSRGGLVVLTRKPLREEESKQKLENSYKAGEHVFGLITGAIKGGVEVDIGGVRAFAPA
                     SGMDLHPAQANFAALVGQRLDFKLVSFDKAGREVVVTRRPMLEAEAHERRKRALALLT
                     EGQVLQGVVRTVVEWGAFVALPGAENLEGLVHVSEASHDPRTPLAELMRPGDRFDVKI
                     IKIDEKGKIWLSRKALIEDPWADAKQKYEVRSRHAGKVTRVEKFGVFVELEPGVEGLL
                     HAADLSFERVEHPEQLLSVGQTIDVLVHHFDARSKKLTLHPAPPADRPEEAAQKVIKS
                     GLVKAEVIKGEAAGVVVRVLGVTGRAARGFIPAGQTGTARGTDLRKAFKPGSIIDVKV
                     IDVDPRSGEPKLSIRGFKEDEERRAHKEYRQKLKEEGGFGTLGDLLRDKLRASHEREM
                     "
     gene            complement(4102..4977)
                     /locus_tag="VFQ35_13210"
     CDS             complement(4102..4977)
                     /locus_tag="VFQ35_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9931650.1"
                     /translation="MTEREVPARPVTADDPVPEVKNVVLVMSGKGGVGKSTVATNLAL
                     ALSRMGLRVGLLDADIYGPSIPTMLGVTGRPNSDGQRIEPIERFGVKLMSIGFLIEDE
                     KSAVVWRGPMLHGALLQFLKDVAWGALDFLLLDLPPGTGDVALTLSQKVRASGAVIVT
                     TPQEVALQDVYKSVSMCQKVGIDILGVVENESYFVCDGCEKRHELFGAGGGQKIAEYS
                     NAPLLGQIPLNPSVRKWGDAGTPVVQAAPGSPAATAFVEVAERLLQAMAEREQGGILD
                     IDRSGGQNRHLPVAR"
     gene            complement(5009..6163)
                     /locus_tag="VFQ35_13215"
     CDS             complement(5009..6163)
                     /locus_tag="VFQ35_13215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931651.1"
                     /translation="MSAPISKSEPQVLAETYALGRRLGEGHAAVVYEAEHLVVGKRVA
                     IKLLRARVTETGARLRFVAETRAAARILHPNVVDIHDLGIAPDGTPYVVMELLAGETV
                     ASLTARKGALPIAQAVEITFQILSGLSAAHRKGIIHGDLRPENVIVMQPRSDQVLVKL
                     LDFGASRALPEAAPLSSAALLYQAPEQLEGNVDERSDVYAAAAVLSRLVRGPAPEARA
                     EAQLPKQLVELIERGLAKKRNERIPSADEFAEQLRTVLAATPSMPNSTTAGAHQVAVV
                     ARRPPGTDASKPPDSAVLRVNLSPRLVTDSLLMSPRLPRAPAPPKLEAGKDFLPMLGD
                     PERDSEDAFRSAPTRTPESERRRLDPRPALLAMAIGFGIGMVIAWLAGLI"
BASE COUNT          940 a         2093 c         2113 g         1142 t
ORIGIN      
        1 gaagtcacgc tggcacgcag tgcccctcgc gtggggggag cctcatccga attcacttcg
       61 gatgaggggg gcggcgcgtg ccgcccccac aaaccaacaa cgacggaggc tgggcccgaa
      121 gggcgccagc ctcctagcgc cccagcctcc tagggccgaa cccaaattcc gaggctgaac
      181 gactggttcg tgaagggcgc gactcgtgca cgcttcaacg cggcttcgat gcacttcgcg
      241 gcgctcgttc ccgcggaggg gccatttacg gctaccgaag agacggtgcc gtcggagccg
      301 aacgtgatct gtgcctgcgt tgcggctcct ccggctacgc acgcgcgggc ggcgcccatc
      361 acgcttccga cggcggcttg caccgcgccc gtcgaggggc ggtccggcag cccgccggac
      421 gtggtgctcc cagctgcggg gcgcatgccg ctcaccccgg ccgcaggtgg ttcggtcgga
      481 gacgccggct tgaccttcgc cggcgtctct tcgagctgaa ttttctccgg cgcggggcgg
      541 accgcggtcg tcgcgcggct cgcgaccgct accggctcgc tgttcgcgtg cgcggggctc
      601 gtcggagaag cgttcttcga tgcgagcggc agcgatgaag ctgcgagcgt cggtggggcg
      661 ctcgctacct cggcgcgcgg cgcgtcttcg gtgggagcga ctgcctgcgc tggagcgggc
      721 gcttgggttg gcgcttgtgc ttgcgcgact ggtgccgcgg gagctgcggc ggtcgctgcc
      781 ggcggcgcgg ccacgacgat ttgcgcgggc tcgcgtcgga aggcgatgac catcagcgcg
      841 gcagcgagcg cggcgcccat gccgccgatc gcaatccatg gcccgagctc cgagcgacgt
      901 gctggtggcg gcgtcgcggc gggtgcgacc gccggtgcga cgcgcgccga cgggggcgcc
      961 ggaggaacgc gagcgtcgac gtcgcgcgtc ggcgacggcc gcgcgctgcg cacccgctct
     1021 acgatgccgt cggtatgaac gcgtgcggca gcggcgagcg agatgctctc tttggcgatg
     1081 tgagccgcct caggacgtgc gtctctttgc gcgaccgaac gtgcgagcgc ggcgaggctc
     1141 gtcgcaggcg ctgggcccgc tgcggcgctg ggcggtgacg acgccggggc ggaacgctgg
     1201 gcgtccgcac ttggcgcggc tcctgaactc ggctcgtccg aagcggtctg cgcctgcgcg
     1261 ccgggctcgg tgggctcatc gggcagggga gccatcagcc acagcggagc ggtgctcttc
     1321 gcttcggttt cgcgcactcg ctgatcgatc tgccgggcga gtccttccca atcgctgcgt
     1381 tccggcgtcg gcagcgcaga aagcagctct tcgacgcgcg gagctaggcg caccggttcg
     1441 tcagacatgg ttctctccgt agccgcgctg agacaagtac tcgcgcagtc ggcgtctcgc
     1501 ttcgtgaatg cgccacgcaa ccgtgccctc gctgcaaccc aaaatctttc cagcgtccgc
     1561 gtggggcagg ccgtcgacga cgacgaggag cagggtcgtg cgcagcacct cgctcagtga
     1621 atcgatccca gcctcgagcg ctaccgcgag ttcgcggtcg gagttcgcgg actccggact
     1681 cgccagcttc gcgcgttgtt cgatgagctg tggctccact cgggggtcgt cggggctgct
     1741 cgagcgctcg ctgtgcttgc gggcccgaat cgcgttcagc gaaaggttca ccgcgattcg
     1801 gtagatccac gtgaacggtt cgctgcgccc atcgaagcga tccagagcac cgtatgctcg
     1861 gacgaacgtg tcctgagtta cgtcttcggc ctcggccgcg ctccgcagta ggtgcagtgc
     1921 cagccgatag actcgctttt ggtgtcggcg cacgacgacg ccgaacgctt cgatatcgcc
     1981 cttttgtgca gcctcgacga gttcgcgatc tgtccgttcc gacatttccg cgtaggggcc
     2041 aaggcccgcg aatcagggta gagggcgcac gatagttcaa caccgcttgg ttggacaatt
     2101 caccgcggaa agccttgggc accggccgtt cggctgatgc tctgagtccc cgtgcgctcg
     2161 caggcaggta gtcaccacct gcgagggtgc atttccaagc cagaggctgc ggcgcccgcg
     2221 cagccgtgcc ttcgcttcac atttcgcgtt cgtgcgacgc gcggagcttg tctcgcagaa
     2281 gatcgccgag cgtgccgaag ccgccctctt ccttgagttt ttggcggtac tctttgtgag
     2341 cgcgccgctc ctcgtcttcc ttgaacccgc gaatgctgag cttgggctct ccagagcggg
     2401 gatcgacatc gatcactttg acgtcgatga tgctgcccgg cttgaaggct tttctgagat
     2461 ccgtgccgcg cgcggtgcca gtctggccag cagggatgaa accgcgggcc gcacgccccg
     2521 tcacgccgag gacgcgcacg acgacgccag ctgcctcccc tttgatgacc tcggccttca
     2581 cgagcccgct cttgatcacc ttctgcgccg cctcttccgg gcgatcggcg ggaggagccg
     2641 gatggagggt gagcttcttg gagcgcgcat cgaagtggtg aaccaggacg tcgatcgtct
     2701 ggccaacgct cagaagctgc tcggggtgct cgacgcgctc gaaggataga tccgccgcgt
     2761 gcaacaggcc ttcgacgccc ggctcgagct cgacgaacac gccgaacttc tccacgcgag
     2821 tgaccttacc ggcgtggcgc gagcgcacct cgtacttctg ctttgcgtcg gcccaggggt
     2881 cttcgatcag cgccttgcgg ctgagccaga tcttcccctt ctcgtcgatc ttgatgatct
     2941 tgacgtcgaa gcgatcaccc gggcgcatca gctcggcgag gggcgtgcgc gggtcgtggc
     3001 tcgcctcgga tacgtggacg agcccttcga ggttctccgc tcctggcagc gcaacgaagg
     3061 cgccccattc gacgacggtt cgtacgacgc cctgaaggac ctgcccctcg gtgagtagcg
     3121 cgagagcgcg cttgcggcgc tcgtgagcct ctgcctcgag catcgggcgg cgagtgacca
     3181 ccacctcacg cccggctttg tcgaagctca ccagcttgaa atcgaggcgc tgaccgacga
     3241 gcgctgcgaa gttggcttgc gcggggtgaa gatccatccc cgacgcgggc gcaaacgcgc
     3301 ggacgccgcc gatgtcgacc tcgacgccgc ccttgatggc gccggtgatg agcccgaaca
     3361 cgtgctctcc ggccttgtag gagttctcga gcttctgctt cgactcttct tcacgcagag
     3421 gcttgcgcgt gagcacgacg aggccgccgc gcgagccgtc gttgtggacg cgcgcaacca
     3481 gccgatctcc aaccgccggg atcaaatcat cgggctccat ctcgccgcga tcgaagatgg
     3541 cgagcgcctt accagagagg tcgaccatga tggcggtctc gagcaccgcc gtgaccttcc
     3601 cgaacacctc ttcaccgacg tggaaaggcg ctcgctcact ggcgcggtgc ggacgctgcg
     3661 catcacgcgc ctccgcctcc tgcgctgccc cctcgggctt cttcttgcgc ttcttgcggc
     3721 ggcgcttctt cttcgccgtc gggtcagaag cgctcgcggc gccttccgct gcagcggact
     3781 caccctcgtc ggacgaatct gcgctgtcgt cggagtcagc gtcgccatca gacgcctggg
     3841 cgtcgtctga tacggcggct tctcgggtgg cctcgacgga ggccagcgcc tcatcgccgt
     3901 tcgcgatttc tggcttttcg gcttgttcgg agctcatggc ggaccggttg cctataacgc
     3961 ggctggggag gaaagggaac gccgacgctt ggcgcactcc ccctagcacg aaatcagagc
     4021 gttgggcggt ccgtgcgcgt ctgtgcagcg ttcccgtgac cgcctaccgc ttccgttacg
     4081 tttcctcgca gtgaaaaacg ctcagcgcgc aacggggagg tgacggtttt gtccgccgct
     4141 gcggtcgatg tcgaggatgc cgccctgctc gcgttcagcc atcgcttgca gcagccgttc
     4201 ggccacttcg acgaaggcgg tcgctgccgg cgaccccggg gcggcctgga cgacgggagt
     4261 cccggcgtct ccccatttgc gcacgctggg gttcagggga atttggccga ggaggggtgc
     4321 gttcgaatac tcggcgatct tctggccgcc gcccgcgccg aagagctcgt ggcgcttttc
     4381 gcacccgtcg cacacgaagt agctctcgtt ttcgacgacg ccgaggatgt cgatgccgac
     4441 cttctggcac atcgaaaccg acttgtagac gtcctgcagc gcaacctctt gcggagtcgt
     4501 gacgatcacc gcgccgctcg cgcgcacctt ctgcgaaagc gtcagcgcga cgtcgccggt
     4561 gcccggcggc aggtcgagca gcaagaaatc gagcgctccc caggccacgt ccttcaaaaa
     4621 ctggaggagc gcgccgtgca acatcgggcc gcgccagacg accgcggact tctcgtcctc
     4681 gatcaaaaag ccgatgctca tgagcttcac gccgaagcgc tcgatcggtt cgatgcgttg
     4741 gccatcgctg tttggtcgac ccgtcacccc caacatcgtc gggatgctcg gtccgtagat
     4801 atcggcgtcg aggagcccca cgcgaagccc catgcggctc agtgcgagcg cgaggttggt
     4861 cgcgacggtg ctcttgccga ccccaccctt gccgctcatc acgagaacga cgttcttcac
     4921 ttcgggcacg gggtcatcgg cggtgacggg acgggcaggc acttctcgct cggtcatggt
     4981 cggggcgtaa gcccagcccg agcgcctgtc aaatcaagcc agcgagccac gcgatgacca
     5041 tgccgatgcc gaacccgatg gccatcgcca acagcgccgg gcgggggtcg aggcgtcttc
     5101 gttccgactc gggagtgcgg gtgggtgcgc tccggaaggc gtcttccgaa tcccgctccg
     5161 gatcgccgag catcgggaga aagtcttttc ccgcttccag cttgggtgga gccggtgctc
     5221 gcggcaggcg cggcgacatc agaagcgagt cggtcaccaa ccggggagac aagttcacac
     5281 gcagtaccgc ggagtcgggc ggcttcgaag catcggtgcc cggtggccgc cgcgcgacca
     5341 ctgcaacctg gtgcgcgccg gcagtcgtcg aattcggcat gctcggcgtc gctgccaaca
     5401 ccgttcgcag ctgttcggcg aactcgtcgg cgctcgggat gcgctcgttg cgcttcttcg
     5461 cgagccctcg ctcaatcaac tccacgagtt gtttcgggag ctgcgcctcc gcgcgcgctt
     5521 ccggagccgg tccgcgaacg agccggctca atacggcggc ggccgcgtac acgtcgctgc
     5581 gctcgtcgac gttgccttcg agttgctctg gcgcctggta aaggagcgct gcgctcgaca
     5641 acggcgccgc ctccggcagt gctcgggacg caccgaagtc gagcagcttg accagtactt
     5701 ggtcgctgcg cggctgcatc acgatgacgt tctcggggcg gagatcgccg tgaatgatcc
     5761 cttttcggtg ggcggcggag aggccgctca ggatctgaaa cgtaatttcc actgcctgcg
     5821 cgatgggcag ggcgcctttc cgcgcggtga gtgacgcgac cgtctctccc gcgagcagct
     5881 ccatgacgac gtaaggagtt ccgtccggag cgatacccag gtcgtgaata tcgacgacgt
     5941 tcggatgtag aattcgagcc gccgcgcgcg tctccgccac gaagcgcagg cgcgccccgg
     6001 tctccgtgac acgcgcgcgt aacaatttaa tcgcgacgcg ctttccgacg acgaggtgct
     6061 cggcctcgta cactaccgct gcgtggcctt cgccgaggcg gcgtccaagc gcataggttt
     6121 ccgcgaggac ttggggctcg gacttcgaaa tcggggcgga catctccttc acacgagcga
     6181 acggcagata ccgcgtgcac gttagagtgc gctggggtga gctgctgtca cggcgcgttt
     6241 gaagcggatt tcactcggct cgtttctgag tttgggtgag ataattca
//
LOCUS       DASTKS010000639         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_102530, whole genome shotgun sequence.
ACCESSION   DASTKS010000639 DASTKS010000000
VERSION     DASTKS010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102530"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFQ35_13220"
     CDS             <1..892
                     /locus_tag="VFQ35_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HET9931652.1"
                     /translation="SVLLDAGAGAAWRFVERNGSEFSRSEGLAVASYHLFMSGALSSS
                     GGELRADAQGLSSFSAAELEKAFQVSSENPLAGVPGRAALVRNLGEVVGTAARYFGAD
                     APRLGNLALFFKRLAVQNRLPARSVLEGVLDALGPIWPGREQCAGKNLGDVFRHSRFG
                     LIPLHKLSQWLTYSLIEALEMEGVRVEALDELTGLAEYRNGGLFVDAGVLELKDPSAR
                     AVAHRVDSDLIIEWRSLTVALLDRVADLLRKQLHLSSTELPLARVLEGGTWHAGRAIA
                     KELRKDGGPPIQIQSDGTVF"
     gene            952..1605
                     /gene="upp"
                     /locus_tag="VFQ35_13225"
     CDS             952..1605
                     /gene="upp"
                     /locus_tag="VFQ35_13225"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9931653.1"
                     /translation="MESQRPFFTTNVTVIDHPLVQHKLTLLRQKERSTSSFRTLLAEI
                     SMLLVYEVTRDLPLTYQEIETPLAKMSAPLIDGKKVVLLNILRAGTGMVEGMLRILPS
                     ARVGQIGLYRDPETLGAIEYYFKLPNEMSDRDVLVVDPMLATGNSAVAAVDRVKTASP
                     RSIKFVCLLAAPEGLINFREAHPEVPVYTAAIDERLNDHGYILPGLGDAGDRLFGTK"
     gene            complement(1681..2553)
                     /locus_tag="VFQ35_13230"
     CDS             complement(1681..2553)
                     /locus_tag="VFQ35_13230"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931654.1"
                     /translation="MNKKYLEISNLEKTYPTPRGPAIIVRDFNLTLAEGEFVSIIGHS
                     GCGKSTVLSIVAGLNKATNGGVILAGREVVEAGPDRGVVFQAPCLLHWLSAIDNVLLG
                     VDEVHAKKSKEERREIARHYLELVGLGDSVDKMPAELSSGMRQRVGLARAFALSPKML
                     LLDEPFGMLDSLTRLELHEVLLAAWRQDRKAALMVTHDVDEALFLSDRIVMMTNGPEA
                     RVGEILTVPFERPRDRAKVMDHPDYYALRERLIGFLEEQGHGRKEPEEPEPARAKPKL
                     WGFLRSFSSGASAR"
     gene            complement(2550..3377)
                     /locus_tag="VFQ35_13235"
     CDS             complement(2550..3377)
                     /locus_tag="VFQ35_13235"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931655.1"
                     /translation="MSILELRSISKSYGATHVLEDVSLQIEPGEFVAIVGFSGAGKST
                     LVSLLNGLQKPDRGEALFKGAPIVGPGPERGVVFQNYSLLPWLTAFENILLAVEQVFT
                     EKTKQQQAEHAQRYVAMVGLSHARDRRPHELSGGMRQRVALARALAAEPEVLLMDEPL
                     SALDALTRATLQDEIERIGRVSGKTIVMITNDVDEAILLADRIIPLSAGPRATLGASH
                     SVDIARPRERVALNHDPHFREVRSSVIEYLMSSKKKRSAPAPEIVPPPMQVQPELGV"
     gene            complement(3389..>3951)
                     /gene="ntrB"
                     /locus_tag="VFQ35_13240"
     CDS             complement(3389..>3951)
                     /gene="ntrB"
                     /locus_tag="VFQ35_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HET9931656.1"
                     /translation="ERVAKGYLIAILLGVPIGFLLGVSNFFRSSFDPIIQILRPVSPL
                     AWLPLGLVIFQKPEPAGVFTIAMCSMWPTVLNTALGVRSVPQDYLNVARVLKLSRAKT
                     LRKVLLPAALPYMFTGFRLSLGIAWLVIVAAEMLTGAPGVGGFLWQEYNALIYEHIIL
                     CIVTIGTVGFVLDRLMGLVEKRLRTA"
BASE COUNT          724 a         1266 c         1280 g          681 t
ORIGIN      
        1 gagcgtcctg ctcgacgccg gtgcgggggc ggcctggcgc ttcgtcgagc gcaatggttc
       61 ggagttttcc cgctcggagg ggttggcggt ggcgagctat cacctcttca tgagcggagc
      121 gttgtcgagc tcgggaggag agctccgcgc ggacgcgcag gggctctcga gcttttcggc
      181 cgcggagctc gaaaaagctt tccaagtatc gagcgaaaac ccgctcgcag gcgtgccggg
      241 tcgcgcggcc ctcgtgcgaa acctcggcga ggtggtcgga accgcggcgc gctacttcgg
      301 cgccgacgcc cctcgcctcg gcaatttggc gctgtttttc aaacgactcg cggtgcagaa
      361 ccgcctgccc gcgcgcagcg tcctcgaggg cgttctcgat gcgctcgggc cgatctggcc
      421 ggggcgtgag cagtgcgcgg gcaagaactt gggcgacgtg tttcggcatt cgcgcttcgg
      481 gctcatcccg ctgcacaagc tgtcgcagtg gctcacctat tcgctgatcg aagcgctcga
      541 gatggagggt gtgcgcgtcg aggcgctgga cgagctgacc gggctcgccg agtaccggaa
      601 cggcgggctg ttcgtcgacg ccggcgtgct cgagttgaaa gatccgagcg cgagggctgt
      661 cgctcatcga gtcgactccg acttgatcat cgagtggcgg agcctcaccg tagcgctcct
      721 cgatcgagtc gccgacctgc tgcggaaaca gctgcacttg tcgtccacgg agctgccgct
      781 cgcgcgcgtg ctggaaggcg gcacttggca cgccggccgc gcgatcgcca aggagctccg
      841 caaagacggc ggccccccca tccaaatcca gagcgacggc accgtctttt gataacctga
      901 tttcgcctct cggaggccaa acccacggcc ccgaagcgcc cagagatttc gatggaaagc
      961 caacgtccct tcttcaccac gaacgtcacg gtcatcgacc acccgctggt tcagcacaag
     1021 ctgactctgc ttcgccaaaa agagcgcagc acgagctcat ttcgcacgct gctcgccgaa
     1081 atcagcatgc tcctcgtgta cgaagtgacg cgcgacctcc ccctcacgta tcaggagatc
     1141 gaaacgcctt tggcgaagat gagcgctccc ctcatcgacg gcaagaaggt cgtcttgctc
     1201 aacatcctgc gcgcgggaac gggaatggtc gaaggcatgc tccgcattct tccctccgcg
     1261 cgcgttgggc agatcggcct ctatcgcgat ccggagacgc tcggcgcgat cgagtactac
     1321 ttcaagctac cgaacgagat gtcggatcgc gacgtgctcg tcgtcgatcc gatgttggcg
     1381 accggcaaca gcgccgttgc cgcggtcgat cgcgtgaaga cggcgagccc gcgctccatc
     1441 aaattcgtgt gcctgctcgc cgcccccgaa gggctcatca acttccgcga ggcgcacccc
     1501 gaggtgccgg tctacaccgc tgccatcgac gagcgcttga acgatcacgg ctacattttg
     1561 ccgggtctcg gcgacgcggg ggatcgtctc ttcggaacga agtgacacct tcgctgcggt
     1621 gcgacctcgc cccgctcgtc gagtcccggc gcgtggcgag cgcagcagcg caatgaccgc
     1681 tcagcgtgcc gaagcccccg aggagaacga gcgcaagaag ccccacagct tgggcttcgc
     1741 gcgtgcgggc tcgggctcct cgggttcctt gcggccgtgg ccctgctctt ccaaaaaccc
     1801 gatcaggcgc tcgcgaagag cgtagtagtc ggggtggtcc atcacctttg cgcggtcgcg
     1861 tggtcgctcg aacggaacag tgagaatttc cccgacgcgc gcctcgggac cgttggtcat
     1921 catcacgatg cggtcggaga ggaagagcgc ctcatcgacg tcgtgcgtca ccatgagcgc
     1981 ggctttccga tcctgccgcc acgctgcgag caaaacctcg tgcaattcca ggcgagtcag
     2041 cgagtcgagc atgccgaaag gctcgtccaa aaggagcatc ttcggcgaga gcgcgaacgc
     2101 gcgcgccagg ccgacgcgct gacgcatgcc ggaagaaagc tcggccggca tcttgtcgac
     2161 cgaatcgccg agaccgacga gctccaagta gtgacgagcg atctcgcggc gctcttcctt
     2221 cgactttttg gcgtgcacct cgtccacacc gagcaacacg ttgtcgatcg ccgagagcca
     2281 atgcaagagg cacggcgcct ggaacacgac tccgcgatcc gggcccgcct ccacgacctc
     2341 tcgccccgcc aaaatgacac cgccgttcgt cgccttgttg aggccggcga cgatcgagag
     2401 caccgtcgac ttcccacagc cggagtgccc gatgatgctc acgaactccc cttcggcgag
     2461 cgtgaggttg aaatcgcgca cgatgatggc cggaccgcgc ggggtgggat acgtcttttc
     2521 gaggttcgaa atctcgagat acttcttgtt catacgccga gctccggttg aacttgcatc
     2581 gggggcggga cgatctcggg agcaggagcc gagcgcttct tcttgctact catcaggtac
     2641 tcgatgaccg aggaacgcac ttcgcggaaa tgagggtcgt gattcaaggc gacgcgctcc
     2701 cgggggcggg cgatgtcgac gctgtgcgaa gcgcctaggg tggcgcgcgg gcccgcgctg
     2761 agcggaatga tgcgatcggc gaggagaatc gcttcgtcca cgtcgttggt gatcatcacg
     2821 atggtcttgc ccgagactct cccgattcgc tcgatttcgt cctgcagagt cgcgcgcgtg
     2881 agcgcgtcga gggcgctgag cggctcgtcc atgaggagca cctctggctc ggcagcgagg
     2941 gcacgcgcga gcgcgacgcg ttgccgcatg ccgccgctga gctcgtgggg ccggcggtct
     3001 cgcgcgtgac tgagccccac catcgcgacg tagcgttggg cgtgctcggc ttgctgctgt
     3061 ttcgtcttct ccgtgaacac ctgctccacg gcgagcagga tgttttcgaa ggccgtgagc
     3121 cacggcagaa gcgagtagtt ttgaaaaacg actccgcgct ccgggcccgg accgacgatg
     3181 ggcgcgccct tgaacagcgc ttcaccgcga tcgggcttct gtagtccgtt gagcagcgag
     3241 accagcgtcg acttgcccgc gccagagaag ccgacgatag caacgaactc accgggctcg
     3301 atctggaggg acacgtcttc gagcacgtgc gtcgcgccgt acgatttcga gatggaccgt
     3361 agctccaaga tgctcattgg ttccgtcctc acgccgtacg cagccgcttt tcgaccaagc
     3421 ccatcaatcg atcgagcacg aaacccacgg tgccgatcgt gacgatgcag aggatgatgt
     3481 gctcgtagat gagcgcgttg tactcctgcc acaagaagcc accgacaccg ggagcgccgg
     3541 tcaacatttc ggccgcgacg atgacgagcc acgcgatgcc gaggctcagc cgaaagccgg
     3601 tgaacatgta gggcaacgcg gccggcagca gcaccttgcg cagcgtcttg gcgcgggaga
     3661 gcttgagcac gcgcgccacg ttcaagtagt cctgaggcac cgagcggact ccgagcgcgg
     3721 tgttcaaaac ggtgggccac atcgagcaca tggcgatggt gaagacgccc gctggctcgg
     3781 gcttctgaaa gataacgagc ccgagcggga gccaggcgag cggcgaaacc ggacgcaaga
     3841 tttgaatgat cgggtcgaag ctcgagcgaa agaagttcga cacgccgagg agaaacccga
     3901 tcggcacgcc gagcaagatg gcgatcaggt agcccttggc cacgcgctcg a
//
LOCUS       DASTKS010000640         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_770520, whole genome shotgun sequence.
ACCESSION   DASTKS010000640 DASTKS010000000
VERSION     DASTKS010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770520"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFQ35_13245"
     CDS             <1..267
                     /locus_tag="VFQ35_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931657.1"
                     /translation="VCESDHDADCRALVCSTDDECPQPGRAPQGSCVGGYCIDPTHEI
                     VSSDSVMLCLAGTGLGHGEARQVERYALGLNCGTPCTVPKPCER"
     gene            complement(349..1482)
                     /locus_tag="VFQ35_13250"
     CDS             complement(349..1482)
                     /locus_tag="VFQ35_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931658.1"
                     /translation="MPVEIRKSPIGVRLDDFLNVVDYIYRGDPSYVRPLNLDLKQRIS
                     ARNPFFDHAEGVLFTAHRNGWCVGRCSAQIDREHLRLHRDDAGFFGFFDTIDDEEVAK
                     ALLDAAASWLAERGMKRMRGPMSFGINEEIGCLVDGFDTPPMVLMPHHRPYQGGLIEK
                     AGLEKLKDVYAWRYTGGDVPTRARKAHDELDALPELKARHCDMKKFDTEIRTVVDVFN
                     DAWADNWGFVPFGERELKKLAEELKLIVIPELTYIAEIDGEPAAVAIAMPNVNELIRD
                     MGGSLSGLAIPKLLWRLKVQGPKTARLAILGIRRKYRGIRKYAGLSTYLYVKMNEAGR
                     RCGIRWGELSWTLEDNAPVNVAIKFMGGKIYKRYRLYERTLGT"
     gene            complement(1571..3184)
                     /locus_tag="VFQ35_13255"
     CDS             complement(1571..3184)
                     /locus_tag="VFQ35_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9931659.1"
                     /translation="MGLRQRIVEKSKGALLNPAVMRWVTDDRVMKATEGLLDARTRVK
                     AAWHVLLNGHELPNIDPALDNTIGTETGRSESKNGNGHVNGNGHVNGSAASVNGNGAA
                     VRINGGASARPDISSGSQDMDESLKERNTLASIGGRDIFEKCWKFMAADNARKMGIYP
                     FFRPLDLNDGPEAVINDRRVVMFGSNNYLGLTTHPRVRDAAKSAIDRFGTSMTGSRLV
                     NGSMKLHEEFEEKLAAYFGKESALVFTTGYQVNIATCAALLSNKYSVAVVDRNVHASL
                     YDGARLGQAAGARLVRYRHNDAESLDKTLEKLDEGEGALVITDGVFSAEGEIAHLDEI
                     VKVVKKHGARLFVDDAHALGVIGPGGRGTAAHFGVTDQVDLIGGTFSKSMASIGGWLV
                     GERKVLDYIRHFAPSFMFAAAAAPPSVAAAMAAFEVMQDEPWRIEKLRENFVYMRTEL
                     AKMGFELGHTQTAVIPIFIRQDLRTIMMWRDLLEEYGIYTNPFISPGVPPKSAMLRTS
                     YMATHSREHLDRGLEAFEKVGKKYGVLKG"
     gene            complement(3296..>3912)
                     /locus_tag="VFQ35_13260"
     CDS             complement(3296..>3912)
                     /locus_tag="VFQ35_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9931660.1"
                     /translation="GVHARGQLVAFDPSHEIPPRGWVLGLARHLPSEIAVVRAAAVEP
                     GFEPRRHVVSKTYRYSLFESPVRDPFLEGRVWRVTERLNHVAMAEAASHLIGEHDFAA
                     FRGAADEREETVRRLFRVDVARARTDERILEIAVRGDRFLYRMVRIIAGTLVDVGRGR
                     IAANATAQALVSKDRRTLGVTAPPDGLCLDEIVLDVAEDQHWPG"
BASE COUNT          644 a         1264 c         1262 g          742 t
ORIGIN      
        1 gtgtgcgaga gcgaccacga cgccgattgc cgagccctcg tgtgctccac ggacgacgaa
       61 tgcccgcagc ctgggcgcgc tccacagggc agctgcgtag gcggatattg tatcgatccg
      121 acccacgaga tcgtgagctc cgactcggtg atgctttgcc tggcgggtac ggggctcggg
      181 cacggcgaag cccgccaagt cgaacgctac gcgctcgggt tgaactgcgg cacgccgtgc
      241 acggttccga agccgtgcga gcgctgacca catccgaatc aacgccgacg tcggcgcgcc
      301 gagcgtgact tcacggccac ccggtgaggc accgggggcc gccgcgcctc acgtcccgag
      361 cgtgcgctcg tagaggcgat accgcttgta gatcttgccg cccatgaact tgatcgcgac
      421 gttcacgggg gcgttgtctt cgagcgtcca cgagagctcg ccccagcgaa tgccgcagcg
      481 gcggcccgcc tcgttcatct tcacgtacag gtacgtgctg aggccggcgt acttgcgaat
      541 gccgcggtac ttgcggcgga tccccaagat tgcgagccgc gccgttttgg gtccttgaac
      601 cttcaagcgc cacaacaact tcggtatcgc gaggccgctc aagctgccgc ccatgtcgcg
      661 gatgagttcg ttgacgttcg gcatcgcgat tgcgaccgcg gcgggttcgc cgtcgatctc
      721 ggcgatgtac gtgagctcgg gaatgacgat gagcttgagc tcctcggcga gcttcttgag
      781 ctcgcgttcg ccgaacggca cgaatcccca gttgtcggcc caagcgtcgt tgaagacatc
      841 gaccaccgtg cgaatctcgg tgtcgaactt cttcatgtcg cagtggcgcg ccttgagctc
      901 cggcagcgcg tcgagctcgt cgtgggcttt gcgcgcgcgg gttggcacgt cgccccccgt
      961 gtagcgccag gcgtagacgt ctttgagctt ttcgaggcca gccttctcga tgagaccgcc
     1021 ctggtagggt cggtggtgcg gcatcagcac catcgggggc gtatcgaagc cgtcgacgag
     1081 gcacccgatc tcttcgttga tgccgaacga catcgggccg cgcatgcgct tcatgccgcg
     1141 ctcggcgagc cagctggcag cggcgtcgag cagcgctttc gcgacctctt cgtcgtcgat
     1201 cgtgtcgaaa aagccgaaga agcccgcgtc gtctctgtgg agccgcaggt gctcgcgatc
     1261 gatctgcgcg ctgcaacgac cgacgcacca accgttgcgg tgggccgtga acagcacccc
     1321 ttcggcgtga tcgaagaacg ggttgcgggc gctgatgcgc tgcttgagat cgaggttcag
     1381 cggccgcacg tagctcgggt cgccgcggta aatgtagtcg acgacgttga ggaaatcgtc
     1441 gaggcgcacc ccgatcgggc tcttacgaat ttctacgggc atgttgcgtc tgagaggctg
     1501 gttttggggc accgggaggc tcgtgcctcc cttaggaccc cggttagccg ttgaaacgcg
     1561 gctgagtcaa tcagcccttg aggacgccgt acttcttgcc gactttttcg aatgcctcga
     1621 ggccgcggtc gaggtgctcg cgcgagtgcg tcgccatgta gctggttcgc aacatcgcgc
     1681 tcttcggcgg cacgcccggc gagatgaacg ggttcgtata gatcccgtac tcctccaaga
     1741 ggtcacgcca catcatgatg gtgcgcaagt cctggcggat gaagatgggg attacggcgg
     1801 tttgggtgtg gccgagctcg aagcccatct tcgcgagctc cgtgcgcatg tagacgaagt
     1861 tctcgcgcaa cttttcgata cgccacggct cgtcctgcat gacctcgaac gccgccatcg
     1921 ccgccgccac gctggggggc gccgccgccg ccgcgaacat gaagctcggc gcgaagtggc
     1981 gtatgtaatc gaggaccttg cgctccccca ccagccaacc gccgatgctc gccatcgact
     2041 tcgagaacgt gccgccgatc aagtcgacct gatcggtgac gccgaagtga gcggccgtac
     2101 cgcgcccgcc tgggccgatg acgccgagcg catgggcgtc gtcgacgaag agccgcgcgc
     2161 cgtgcttctt cacgaccttc acgatctcgt cgaggtgggc aatctcgcct tcggcgctga
     2221 aaacgccgtc ggtgatcacg agcgcgccct cgccctcgtc gagcttctcg agagtcttgt
     2281 cgagcgactc ggcgtcgttg tggcggtagc gaacgaggcg ggcgcccgca gcctgaccga
     2341 gccgcgcgcc gtcgtagagc gaagcgtgca cgttgcgatc gacgacggcg acgctgtact
     2401 tgttgctgag cagcgcggcg caagtcgcga tgttcacctg gtaaccagtg gtgaacacga
     2461 gcgcgctctc tttgccgaag tacgccgcga gcttctcttc gaactcctcg tgaagcttca
     2521 tcgagccgtt gacgaggcgt gagccggtca tgctcgtgcc gaagcgatcg atcgcgctct
     2581 tcgccgcgtc gcgcacgcgc ggatgcgtgg tgaggccgag gtagttgttc gagccgaaca
     2641 tcacgacgcg acggtcattg atcaccgcct cggggccgtc gttcaagtcg agcggacgga
     2701 agaagggata gattcccatc ttgcgcgcgt tgtccgcggc catgaacttc caacacttct
     2761 cgaagatgtc gcggccgccg atgctcgcga gcgtgttgcg ctccttcagc gactcgtcca
     2821 tgtcttgcga gccgctcgag atgtcggggc gcgccgatgc gccgccgttg atgcgcacgg
     2881 ctgcgccgtt gccgttcacg ctggcagcgc tgccgttcac gtggccattt ccgtttacgt
     2941 ggccgtttcc gttcttgctc tcgctgcggc ccgtctcggt gccgatcgtg ttgtcgaggg
     3001 ccgggtcgat gttgggcagc tcgtgtccgt tgaggagcac gtgccaagcc gccttcacgc
     3061 gggttcgcgc gtcgagcagc ccctcggtcg ccttcatcac gcgatcgtcc gtgacccaac
     3121 gcattacggc tggattgagg agcgcaccct tgctcttctc gacgattcgt tgcctaagtc
     3181 ccattggagt cctacgtcgc ccgctttgcc tcgtacgaga ggaccgggtc gaccctgagc
     3241 gggtcgcgaa ccgggactga gcgataacgg acgctacgtt gcaatccgac cttcgtcaac
     3301 ctggccaatg ctgatcttca gccacgtcga ggacgatctc gtccaggcac aacccgtctg
     3361 gaggagccgt aacgccgagc gtgcgtcggt ccttcgagac gagcgcctgt gcggtagcgt
     3421 tcgcggcgat tcgcccgcgt ccgacatcga cgagtgttcc cgcgatgatg cgaaccatgc
     3481 ggtaaagaaa gcgatcgccg cgcaccgcta tttccaagat gcgttcgtcc gttcgcgctc
     3541 gcgcgacgtc gacgcgaaat agccgacgca ctgtctcttc gcgttcgtcg gcggcgccac
     3601 ggaacgccgc gaagtcgtgt tcaccaatta gatgactcgc agcctccgcc atcgcgacgt
     3661 ggttcaacct ctctgtgact cgccagactc gaccctcgag aaaaggatcg cgcacgggac
     3721 tctcgaacaa cgagtagcga taagtcttcg agaccacgtg acggcgcggc tcgaacccag
     3781 gctcgacggc ggcggccctt accacggcga tttcagaagg caagtgccga gcgaggccga
     3841 gcacccaacc ccgcggagga atctcgtggc tcggatcgaa ggcgacgagc tgcccccgcg
     3901 cgtggacgcc cg
//
LOCUS       DASTKS010000641         9485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1442025, whole genome shotgun sequence.
ACCESSION   DASTKS010000641 DASTKS010000000
VERSION     DASTKS010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442025"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..561
                     /locus_tag="VFQ35_13265"
     CDS             61..561
                     /locus_tag="VFQ35_13265"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET9931661.1"
                     /translation="MTKRIRFSLTSKVFGVTALVACVALFNSRVSANAPVGRYVIGTG
                     ATAGTVYDSVTALTWMRAPIENPIDWVGATTYCSDLTFGGSSDWRMPTVKEAATLVDE
                     SRYYPAVDSDAFPAMPSGYFWTGTLIPWDSTHAQTLHTGSGTIGVAYPLLDGTNKAVL
                     RAICVH"
     gene            complement(664..1638)
                     /locus_tag="VFQ35_13270"
     CDS             complement(664..1638)
                     /locus_tag="VFQ35_13270"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HET9931662.1"
                     /translation="MRSNPSYASCSLLVFVTSACAVDLGPKEQAPPPGALAAVLPDCL
                     PLTRTLESLEGRLDAQRLPDGRLLLGAGDRAFSVESPRAACDDVFDAAFEAPLFDGRP
                     IGVDGAVTLLAGVEGYRFFSAEGRGLGVAEWNAERRSFEARALLFTADRPRFGSAATR
                     FGDHVYVFGGLPARFLAADVYLARVLMSDVAEPAAYEYWQGGGGWTSDADLAAPIVEG
                     GTSPSVLFDAAHDRWLMAYATPLASEIQLRSGLGVTGPWSLPLVVGRCRLPPSDPNAF
                     CDDVTLLPAMETNSLNLGYRIGSFTSAPRNPREYWTRLVTTTWPTTLP"
     gene            complement(1628..2371)
                     /locus_tag="VFQ35_13275"
     CDS             complement(1628..2371)
                     /locus_tag="VFQ35_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931663.1"
                     /translation="MTSYAWGPRARASLGADFTLLRLSHSRLGTRALLAFEDADEKRF
                     FPADVGRAAIEIGFAWPLAALSKRRALELGVSIGRETAFSLEGFVLRDRFHSDDVPFG
                     AGGWYSKGDVAFRSAVGSDWLVSTRVGLHLYTNLFPALVGARDASDVVADHLREGAEL
                     KTTFELGVRYMKSACYQPLARVYLDSIAPHDDSAHPLWLGRLLLAVALPREALEFQPF
                     FDAEAGHGSGLLVNRNQLRFGAGVRLHAK"
     gene            complement(2485..3606)
                     /locus_tag="VFQ35_13280"
     CDS             complement(2485..3606)
                     /locus_tag="VFQ35_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HET9931664.1"
                     /translation="MRIARLRAEERFAASRRQLCSPRARYGRLVAATFALSMAGCAPD
                     GPAWHAEPWPEANALFTNDPRWIGGDGAYTVDLGRGRILWLFGDSFIALSSERRRDDS
                     YMVRNSVAIQTGCDPSTAFMRFYYRTLERHPQSFIPEDGKFWFWPGHGVRIGDRALLF
                     YGRVFQEGEGMWGFSSGSWTAFVVENPDDEPIAWRYREARTAADTEVQLGGAVLPIGD
                     WLYVYGNGTGRHPIYLARFDTQRAMQGDLTSPEWWNGEGWGGSREALIEIGAPEYSVH
                     RVPGTTTFAFTQTAGFGASTLAVRTANALEGPWSAPRDIIRPPESFLPEPFVYAGKAH
                     PELCGADVAVTYVPSGFDTSPPDPKELYYYPRFVRVSYQ"
     gene            complement(3717..4370)
                     /locus_tag="VFQ35_13285"
     CDS             complement(3717..4370)
                     /locus_tag="VFQ35_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931665.1"
                     /translation="MTERRDSGARHPLFRAMVLMGGSLALHCGGNARHDEAGSGGNPA
                     GGAAGTGGQRTSGGTSLGGATVSGGTSGATGSGGAGNANGSGGSLPKGGEAGGPPNPV
                     TPGPFDCPPAQWDCSAIPPACTGEYFLLPEGCACDTTRPKTPEECGDADWVCRHAEFD
                     SQRRLFTTPVDFECFCAPHQPTCREACSMAYGYPSRCANELPATNAVLCDCAIIVLR"
     gene            complement(4367..5038)
                     /locus_tag="VFQ35_13290"
     CDS             complement(4367..5038)
                     /locus_tag="VFQ35_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931666.1"
                     /translation="MYGLSAEQCETLAVYWWRRAEGEMTSWVGFRHVLADLQAEGSPA
                     AILSLAERAVRDEHRHALWCRDFAVRFGYPAPPEVKPRSEARLEFRGATAAQNRLLRI
                     LLCCLTETVGCFILRHVRGVMREPEFLRNNQTHLADELQHSRVGWGHLATLEAHQRDF
                     LREWSPRLLALLPVACCEGPELELPELVPFGYFTPSVLRAAHDEAIDEVILPGLQHLG
                     LTEAA"
     gene            complement(5233..6600)
                     /locus_tag="VFQ35_13295"
     CDS             complement(5233..6600)
                     /locus_tag="VFQ35_13295"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9931667.1"
                     /translation="MSNTESAPLVLVVDDHFEMAETVAAGLVSRGFRGVAIGSAEEAL
                     RQIASGQHAALVTDLRVPGSDGLELLARSKLSAPESPVVVMTAFSDVDTAVESIRRGA
                     YHYMTKPFKMDELALFLRRALDDSAVRREARELRGALHRPLDSLVGESPPMLDVFDLV
                     RRVARSNAPVLVLGETGTGKGMVARALHTEGGRAHAPFVTVNCAAIPEALLESELFGH
                     VKGAFTGAARDHVGLFEQAHRGTLFLDEIAEMSPVLQSKLLHVLESGRIRPVGSTSER
                     QVDVRIVAATHRSLKQRAAQGTFREDLLYRLDVISIELPPLRHRLTDLPKLIAHFAAE
                     SQQKNPGFPRLRLSAEVMNVLTAHDWPGNVRELKHLIERLTLLCNNGEATLRDLPLDF
                     AGRQTPKRDLTFGGKVIPMRELQRRYVSWALEQLGGAKMATCEALGIDSKTLNRWLRD
                     NSEED"
     gene            complement(6600..7979)
                     /locus_tag="VFQ35_13300"
     CDS             complement(6600..7979)
                     /locus_tag="VFQ35_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9931668.1"
                     /translation="MISRLLDRLKKTSRRARTVFSTALGVVTVAAAGIWDAQRQDEFA
                     LNELIHAHHLLATALATRIAREPVRGLQEGRAFGEQVLAAGRVLGAEGNVVVMLQTPE
                     RREFSLSDGRVIELPDLERVLARNEQGVTLSRDVAGDLGLRQRAAVAGVARFRWPSGG
                     EAAVAVVGSAGAERDRRRHDQWRTVLTTLLVGGLVLGSGAAALRRQRRELDLERRLAV
                     HRTERERDAELARADRMAAIAAISSGIAHDIATPLGIISGRLEQLTQLLGTADATTKL
                     IDSIAQQVDRINKVIRGLLGLARGDSPTLMRVAPAKLASEAAQLVKHRFAVANVELRV
                     NVLVADDRLVACDTALFEQVLVNLLVNALEASSSGQLVELRVEEGERQVYFSVIDQGS
                     GISETAISRATEPFFTTKARQGGSGLGLAIARQIVAHHRGQLSVARRRSGEGEGTGTL
                     ALVALPESS"
     gene            8084..9481
                     /locus_tag="VFQ35_13305"
     CDS             8084..9481
                     /locus_tag="VFQ35_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9931669.1"
                     /translation="MLVSCPRSIRLGVALFTAALSSAGRAAAEEPASIRALALNAAVD
                     FALKHQPEVLAARKRLIASRADAEVPIAQWQPNVGLVAEALLGTANNSTASILSPRTV
                     DLPRIGGTSVEENPSARPYGSSLVALGVRQEVFDFGRIAAQAAAVRALAATDKERLRA
                     QRLDTVLSVTEAYYAVLAARTVVEVAGQAEARARMHRDYADAGVRSGLRSPIELTRAE
                     ADVTRFEVNRIRAEGGLRVARSVFAAAVGVDDPELDAGPDTSAPFQLPDHALVQARAR
                     DADPAVRVAVSRSRAQDAVTAVLKASERPNIYASAAVSGRAGGAPASDGVVPQGDGAL
                     PIVPNYSAGLVLEWPMYDAVTLARERASKQRELALLAEVDVARQRAVTRSEQAYRDAE
                     VALRALDALSRAAEAARANHDQAEARFKQGLGTSVELADAETLRIDAELQLAIGKFQL
                     AVDRAVLLRTMTEEP"
BASE COUNT         1623 a         3172 c         3143 g         1547 t
ORIGIN      
        1 cggagcgcgc agccatcgtt cgttgcgtgc actgaaccct cgacgaaagg agcaaatacc
       61 atgaccaaaa ggatccgctt ctccctcacg tcgaaggtct tcggcgtcac cgcgctcgtc
      121 gcctgcgtcg cactcttcaa ttcgcgagtc agcgcgaacg cgccagtcgg acgctacgtg
      181 atcgggacgg gcgctactgc cggcactgtc tacgacagcg tcaccgcgct aacctggatg
      241 cgcgcgccga tcgaaaatcc gatcgactgg gtgggcgcga ccacctactg cagtgatctc
      301 accttcggcg gctcttcgga ttggcgtatg cccaccgtga aggaggctgc gacgctggta
      361 gacgagagcc gctattaccc ggccgtcgat tccgatgctt ttcccgcgat gccttccggg
      421 tacttttgga cggggacgct catcccttgg gactcgacgc acgctcagac ccttcacacc
      481 gggtccggca cgatcggggt cgcctatcca ctgctcgacg gcacgaacaa ggcagtgctc
      541 cgcgcgatct gcgtgcactg ataccgatgc agtgcaagcg cgcagcgagc gtcacggccc
      601 gctgcgtgcg gccgagctcg cggaggcgtg aacgccgcga gcctcgaacg gcggtgcacg
      661 cgctcacggc aacgtcgtgg gccaagtcgt cgtcacgagt cgcgtccagt actcgcgagg
      721 gttgcgcggg gcgctcgtga agctcccgat gcggtagccg aggttcaatg aattcgtttc
      781 catggctggg agcagcgtga cgtcgtcgca gaaggcgttg gggtcgctcg gaggcaagcg
      841 gcaacgtccg acgacgagcg gaagcgacca gggtccagtg actccaagcc cactgcgaag
      901 ctggatttcg cttgctaggg gtgtcgcata ggccatcagc cagcggtcgt gcgcggcgtc
      961 gaagaggaca cttggggacg taccaccttc gacgattggt gcggcgagat ccgcatcgga
     1021 tgtccagccg ccaccgcctt gccagtactc gtaggcggcg ggctcggcga cgtcgctcat
     1081 gagcacccga gcgaggtaga cgtcggcggc gaggaaccgc gccggcaacc ctccgaagac
     1141 gtaaacatgg tcgccgaagc gcgttgcggc gctaccgaag cgcgggcggt ccgccgtgaa
     1201 aagcagggcg cgcgcctcga acgagcggcg ctcggcgttc cactcggcga cgccgagacc
     1261 gcgtccttca gccgaaaaga agcggtaccc ttcgacccca gcgagaagtg tgactgcgcc
     1321 gtccacacca atgggtcgcc cgtcgaagag cggcgcttcg aacgcggcgt cgaagacatc
     1381 gtcgcacgca gctcgcggag attcgacgct gaacgctcga tcgcccgcgc cgagcaggag
     1441 gcgcccgtcc ggcagccgct gcgcgtcgag acgaccttcg agagattcga gcgtgcgagt
     1501 cagcggcaga caatctggga gcacggccgc gagcgcgccc ggtggcggag cctgctcctt
     1561 gggcccgaga tcgaccgcgc acgccgaggt cacgaaaacc agcagtgagc acgacgcata
     1621 cgacgggtta cttcgcatgc aacctcacgc ccgcgccgaa gcgcagctgg ttgcgattga
     1681 cgaggaggcc gctgccgtgg cccgcctcgg cgtcgaagaa cggctgaaat tcgagggcct
     1741 cgcgggggag ggcgacggcc agcaacaacc gcccgagcca gagcggatgt gcgctgtcgt
     1801 cgtggggggc gatgctatcg aggtaaacgc gagccagcgg ctgataacac gcgctcttca
     1861 tgtagcgaac gccgagctcg aatgtagtct tcagctcggc gccctcgcgc aagtgatcgg
     1921 ccacgacgtc gctcgcatcg cgcgcgccga cgagagcggg aaacaggttc gtgtagaggt
     1981 gcagtccgac gcgcgtgctg acgagccaat cgctcccgac cgccgagcga aatgcgacgt
     2041 cacccttcga gtaccaacca ccggcaccga agggcacgtc gtcgctatgg aagcgatcgc
     2101 ggagaacgaa gccctcgagg ctgaaagcag tttcacgccc gatgctcacg ccgagctcga
     2161 gcgcgcggcg cttcgagagc gcggccagag gccaggcgaa gccaatttcg atcgcggcgc
     2221 gtccaacgtc ggcgggaaaa aagcgtttct cgtctgcgtc ttcgaacgcg agcaaggcgc
     2281 gagtgccgag acgcgagtgg ctcagccgca gcagcgtgaa atctgcgccg aggctcgctc
     2341 gcgcgcgggg accccatgcg tagctcgtca cgggcgcgtc cggggacgcc gaatctgcca
     2401 ctgaaatggc gcggctcggt tcggcgtgag ccaggcgcgc gcaaagtacg aatccgctcg
     2461 caagccaagt ggagacatgg cgcctcattg gtaactcacc ctcacgaacc gcggatagta
     2521 atagagctct ttcgggtcgg ggggtgacgt atcgaagccg gacggcacgt acgtgaccgc
     2581 aacatcggcg ccgcatagct ccgggtgcgc cttgcccgcg tacacgaatg gctccggcaa
     2641 gaacgactcc ggcggtcgaa tgatgtcgcg aggggcggac catggcccct ccagtgcgtt
     2701 cgccgtgcgc acggccagcg tgcttgcgcc gaaaccagcc gtttgggtga acgcgaacgt
     2761 ggtcgtcccg ggcactcggt gcacgctgta ctccggggcg ccgatttcga tcaacgcctc
     2821 gcgcgaacct ccccatccct cgccgttcca ccattcgggg ctcgtgagat cgccttgcat
     2881 tgcgcgctgc gtatcgaagc gagcgagata gatgggatgg cggccagtgc cgttgccgta
     2941 gacgtaaagc caatcgccaa tcggcagcac ggcgccgccg agttgcacct cggtgtcggc
     3001 ggccgtccgt gcctctcgat agcgccacgc gatgggctca tcgtctgggt tttcgacgac
     3061 gaatgcagtc cagctgccgc tcgagaaccc ccacatgcct tctccctctt ggaagacgcg
     3121 cccataaaag agcaacgcgc gatcaccgat gcgtacgccg tggcccggcc aaaaccaaaa
     3181 ctttccgtcc tcggggatga agctctgcgg gtgacgctcg agagtgcgat agtaaaagcg
     3241 catgaacgcg gtggacggat cgcacccggt ttgaatcgcg acgctgttcc gcaccatgta
     3301 cgagtcgtcg cgccgccgct cgctcgagag cgcgatgaac gaatcgccga acagccagag
     3361 gattcgcccc ctcccgaggt cgaccgtgta cgccccatcg ccgccgatcc aacgcggatc
     3421 gttcgtgaag agcgcgttgg cttcgggcca cggctcggcg tgccatgcgg gcccgtccgg
     3481 cgcgcacccc gccatcgaaa gcgcgaacgt ggccgcgacg aggcgaccgt aacgcgcgcg
     3541 cggcgagcaa agctgcctgc ggctcgcggc gaagcgttct tcggctcgga gtctcgcgat
     3601 gcgcatcgac aaacgagtag cgtctcttcc cacccgcgcg caacgtgccc ctctcgcgcg
     3661 cccttagtca gtgctcattg ggcctgactg acgaaacccg caagaagcaa tgcgtgtcag
     3721 cgcagcacga tgatggcaca atcgcagagg acggcgttcg tcgcgggcag ctcgttcgcg
     3781 cacctcgatg gatagccgta agccatgctg caagcctccc tgcacgttgg ctggtgcgga
     3841 gcgcaaaagc actcgaagtc gacgggagtc gtgaagagcc tcctctgcga gtcgaactcc
     3901 gcgtggcggc acacccaatc tgcatccccg cactcttccg gcgttttcgg ccgcgtcgtg
     3961 tcgcacgcac agccctcggg caacaagaag tactcgcccg tgcaagcggg aggtatcgcg
     4021 ctgcaatccc actgtgctgg cgggcaatcg aagggtccgg gcgtgaccgg attgggcggc
     4081 ccgcctgcct cgccgccttt tggtaagctc cccccgctac cgttcgcatt acctgctccg
     4141 ccgctgccgg ttgcaccgct cgttccgccg ctgaccgttg cgccgcccaa cgaggtccct
     4201 ccgctcgttc gttggcctcc ggtgcccgcc gctccgcccg cagggttgcc cccgcttccg
     4261 gcttcgtcgt ggcgcgcgtt gccaccgcaa tgaagcgcca gactaccgcc catcaacacc
     4321 atggctcgaa agagcggatg gcgcgctcca gaatcgcgac gctcggtcat gctgcctccg
     4381 tgagtcccaa gtgctgaagc ccgggtaaaa tgacttcgtc gatggcttcg tcgtgcgccg
     4441 cgcgcaagac gctgggcgtg aagtagccga acggtaccaa ctcgggcaat tcgagctccg
     4501 gcccttcgca gcatgcgacg ggcaagaggg cgagcagccg tgggctccac tcgcgcaaaa
     4561 agtcgcgctg atgcgcctcg agcgtggcca aatgccccca cccgacacga ctgtgctgaa
     4621 gctcgtcggc caagtgcgtt tggttgtttc gcaaaaactc gggctctcgc atgacgccgc
     4681 gcacgtggcg caagatgaag cagcccaccg tttcggtgag gcaacacagg agaatcctga
     4741 gcagccgatt ctgagctgcg gtggcgcctc gaaattcgag ccgtgcttcg ctccggggct
     4801 tgacctcggg gggagctggg tagccgaaac gaacggcgaa gtcgcgacac cagagagcgt
     4861 gccggtgctc gtcgcggacg gcgcgctcgg ccaggctcaa gatcgccgcc ggagacccct
     4921 ctgcctgcaa gtcggcgagc acgtgtcgaa agccgaccca cgacgtcatc tcgccctccg
     4981 cgcgccgcca ccaatacacg gcgagggtct cgcattgctc cgcgctcaag ccatacaccg
     5041 tgacagggtc tcacgtgtcg cggcacgact caagcaaagc agccgcggaa ggtaccggcg
     5101 aaccaagcaa cccctgaata ccccacggag tttatgcccg cgcctacatc atgcttttcg
     5161 catgactcag atgcagggct cgtgacgtgc gggcgcgccg gtttacgaat tctcgcgcga
     5221 ccttttcccc gatcaatcct cttcgctgtt gtcgcgaagc cagcgattca gtgtcttcga
     5281 gtcgatgccg agggcctcgc aggtggccat ctttgcgccg ccgagctgct cgagggccca
     5341 gctgacgtag cgccgctgca gctctcgcat cgggatgact ttgcctccga acgtgaggtc
     5401 gcgttttggc gtttgtcgtc ccgcgaagtc gagcggcaaa tcgcgcaggg tggcctcgcc
     5461 gttgttgcac agcaacgtga gtcgttcgat gaggtgcttg agctcgcgca cgttgccggg
     5521 ccagtcgtga gccgtcagca cgttcatcac ctccgccgac agccgcagcc gcgggaagcc
     5581 agggtttttc tgctgagact cggccgcgaa atgcgcgatg agtttgggca agtcggtcaa
     5641 gcgatgacgc agcggaggca gctcgatcga gatcacgtcg agccggtaca agaggtcttc
     5701 gcggaacgtc ccttgcgcgg cgcgctgctt gagtgagcgg tgcgtcgcgg cgacgatgcg
     5761 gacgtcgact tgccgttccg aagtgctgcc gacggggcgt atgcgtccgc tctcgaggac
     5821 gtgcagcagc ttggactgga gtacagggct catttccgcg atttcgtcga ggaagagcgt
     5881 gccgcggtgc gcctgctcga agagcccgac gtgatcgcgc gcggcgccgg tgaacgcgcc
     5941 cttcacgtgg ccgaacagct cgctctcgag caaggcctcg gggatcgccg cgcagttgac
     6001 ggtgacgaac ggcgcgtgcg cgcgcccgcc ctcggtgtgg agagcgcgcg cgaccatgcc
     6061 ttttccggtg ccggtctcgc ccagcaccag caccggcgcg ttcgagcgcg ccacccggcg
     6121 aacgaggtcg aacacgtcga gcatcggcgg gctctcgccg accaagctat cgagtgggcg
     6181 atgcagcgcg ccgcgcagct cgcgtgcctc gcgacgaacc gccgagtcgt cgagcgcgcg
     6241 ccgcaagaag agcgcgagct cgtccatctt gaacggcttc gtcatgtagt ggtacgcgcc
     6301 acgtcggatc gactccaccg cagtgtcgac gtcgctgaac gcggtcatca cgacgacggg
     6361 gctctcgggg gcggagagct tcgagcgcgc caagagctcg aggccgtcgc tgcccggcac
     6421 gcgcaggtcg gtcacgagcg ccgcgtgctg gccgctcgcg atttggcgca gcgcttcttc
     6481 ggcgctgccg atcgcgacgc cgcgaaaccc ccggctcacg aggccggccg ccaccgtttc
     6541 ggccatttcg aagtggtcgt ccacgacgag gacgagcgga gcggattcgg tgttcgacat
     6601 cacgagctct ccggaagtgc gaccagcgcg agcgtccccg tgccctcgcc ttcgccgctg
     6661 cgccgacgcg cgaccgacaa ctgtccccgg tggtgggcca cgatttgtcg ggcgatggcc
     6721 aacccgagcc cgctgccacc ctgacgggcc ttggtcgtaa agaaaggttc cgtcgcgcgc
     6781 gatatggccg tctccgagat tccggagccc tgatcgatca ccgaaaagta cacctgccgc
     6841 tctccttcct cgacgcgcag ctctacgagt tgaccagagc tcgaagcctc gagcgcgttc
     6901 accagtaggt tgaccagaac ttgctcgaac agggccgtgt cgcacgccac gagccggtcg
     6961 tctgcgacga gcacgttgac tcggagctcg acgttggcga ccgcgaagcg gtgcttcacg
     7021 agttgcgcag cctcgctcgc gagctttgcc ggtgcgacgc gcatgagcgt cgggctgtct
     7081 ccgcgcgcca gcccgagcag cccgcggatc actttgttga tgcgatccac ctgttgagcg
     7141 atgctatcga tgagcttcgt ggtggcgtcg gccgtaccga gtagctgggt gagctgttcg
     7201 agacggccgc tgatgatgcc gagcggcgtc gcgatgtcgt gcgcgatacc tgaagagatc
     7261 gccgctatcg ctgccatgcg gtcggcgcgc gcgagctctg cgtcgcgctc gcgctcggtg
     7321 cggtgaaccg cgagccgccg ctcgaggtcg agctcgcggc gttgtcgccg cagcgccgcc
     7381 gcgcccgatc ccaaaacgag accgccgacc agcagtgtcg tcagcaccgt gcgccactgg
     7441 tcgtgccttc ttcggtcccg ttcggcgccc gcgctaccca ccacggccac ggctgcttca
     7501 ccgccgctcg gccagcggaa gcgagcgacc cccgcgaccg ccgcgcgctg cctcagcccc
     7561 aaatcgccgg cgacgtctcg gctcagggtg actccctgct cgttgcgcgc gagcacgcgt
     7621 tcgaggtcgg gcaactcgat cactctgccg tcggacagag agaactcgcg gcgctccggc
     7681 gtctgcagca tgactactac attgccttcc gcgccgagta cgcgaccggc ggccaagact
     7741 tgctcgccga aggcgcgccc ctcctgcaaa ccgcgtacgg gttctcgcgc aattcgggtc
     7801 gccaacgcgg tggctagcag atggtgcgcg tgaatcaatt cgttcagcgc gaactcgtcc
     7861 tgccgctgcg catcccaaat tccggccgcg gcgacggtca cgacgcccag cgccgtcgag
     7921 aatacggtcc tcgctcgacg agaagttttt ttcaatcggt cgagcaatct tgagatcacg
     7981 gagattctcc tagtgcattc ggaaattttc cgagtacgaa ctcgcgaacc tcggggttta
     8041 ttcggggaga cgtgttggcg cgccgcttgc tctcaggcgc cgcatgctcg tttcgtgccc
     8101 tcgttcgatt cgtctgggag tcgcgctgtt tacggcggct ctgtccagtg cgggtcgagc
     8161 ggccgcggaa gagcccgcgt cgattcgagc gctcgctctg aacgccgcgg tcgactttgc
     8221 gttgaagcat cagccagaag ttctagcagc tcgaaagcgg ctgatcgcct cgcgcgccga
     8281 cgcggaggta ccgatcgcgc agtggcaacc caacgtgggg ctcgtcgctg aggcgctgct
     8341 cgggaccgcc aacaactcga ccgcaagtat tctgtcgccc cgaaccgtcg atctcccgcg
     8401 catcggaggc acgagcgtcg aggagaatcc gagcgcgcga ccttacggga gcagtctcgt
     8461 cgcgctcgga gtccgacaag aggtgttcga cttcgggcgc atcgcggcgc aggcggccgc
     8521 ggtccgagct ctcgctgcga ccgacaagga gcgcctgcgc gcacagcggc tcgacaccgt
     8581 gctcagtgtg acagaggcct actacgccgt actcgccgcg cgcacggtgg tcgaggttgc
     8641 gggtcaggcc gaggcacggg cgcgcatgca tcgcgactac gccgacgcag gagttcgctc
     8701 cggattgcga tcgcccatcg agctcacgcg agccgaagcg gacgtgaccc gcttcgaggt
     8761 gaatcgcatc cgcgccgaag gcggcttgcg cgtcgcgcgc agcgtgttcg cggcggcggt
     8821 gggcgtcgac gatcccgagc tcgacgcggg ccccgatacg agcgcgcctt tccagttgcc
     8881 ggatcacgcg ctggtgcaag ctcgcgcgcg cgacgcggac cccgcggtga gggtcgcggt
     8941 ttcgcggagt cgggctcaag acgcggttac cgcagtgctg aaggcctcgg agcggccgaa
     9001 catctacgcg agcgccgccg tctcgggacg ggcgggcgga gcgccggctt ccgacggcgt
     9061 cgttccgcaa ggtgacgggg ccctgcccat cgtgccgaac tacagcgctg ggttggtgct
     9121 ggagtggccg atgtacgacg cggtgacgct cgcgcgcgag cgcgcttcca agcagcgcga
     9181 gctcgcgctc ttggccgagg tcgatgtcgc gaggcaacgc gcggtgacgc gctccgagca
     9241 ggcttaccgc gacgccgagg tggcgttgcg cgcgctcgac gcactttcac gcgcggcgga
     9301 ggcggcgcgc gcgaaccacg atcaggccga ggcccgcttc aagcaagggc tcggcacgag
     9361 cgtcgagctc gccgacgcgg agacgctccg catcgacgcg gagctccagc tggcgattgg
     9421 caagttccag ctagcggtag atcgcgccgt tctcttgcga accatgacgg aggaaccatg
     9481 agcga
//
LOCUS       DASTKS010000642         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_103143, whole genome shotgun sequence.
ACCESSION   DASTKS010000642 DASTKS010000000
VERSION     DASTKS010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103143"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFQ35_13310"
     CDS             complement(<1..306)
                     /locus_tag="VFQ35_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9931670.1"
                     /translation="MRVIVLVKANKESEAGQMPSTEALAAMGRFNEELVKAGVMLAGE
                     GLHPTSRGKRVVIGSSGKRSIIDGPFTETKELVAGFWIWQVKSLEEAVEWAKRIPSPG
                     "
     gene            complement(468..1559)
                     /locus_tag="VFQ35_13315"
     CDS             complement(468..1559)
                     /locus_tag="VFQ35_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9931671.1"
                     /translation="MDFRIERLHREAAKQEQALSLAGGLPLASLFPRDELFGAAEQLN
                     DGARDEALQYTWPEGSEVLREWVAQGLRARGAPVRASDVLITNGAQQALSLLVSALGL
                     SRARIAADELSYPGAIDLFRHAEVRLVTEGPVVARYVIPGASNPLGLGLDSRRRQQLL
                     ASGVPLIADEAYAELRYDGMPERPLLADAPDRVFHVGTLSKTLCPGLRVGWLIAPPGF
                     RGALLEAKRDADLQAPSLTQALAERVLTRLDWSAHLKRAREAYVCRVTRLMRSVRRWL
                     PGFSFVEPEGGFTLFIQSNEPGLDEMKLLELATDFGTSFDPGRMFRVDEDSPGPCAMR
                     ISPCNIPEDRIDEAVQRLSRAVTACRKAA"
     gene            complement(1573..1962)
                     /locus_tag="VFQ35_13320"
     CDS             complement(1573..1962)
                     /locus_tag="VFQ35_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931672.1"
                     /translation="MSRRLIPLLSALALPGCFFVSESDHHSSDGTVVVDWTIHGYKDS
                     SLCTLGDADAIDVVIDRGDWSYETTQSCRAFSVSIDLAPGTYSGIAVLLDPSGADRTT
                     EVDLGTFTIYGNDVLSVPIDFPAASFY"
     gene            complement(2006..2422)
                     /locus_tag="VFQ35_13325"
     CDS             complement(2006..2422)
                     /locus_tag="VFQ35_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931673.1"
                     /translation="MLRTSLIVSFFNSPWLKLAVLLAAGATSSGCYARTQGYVVAEAP
                     PAYVETYPSYYYEGRTVYLVDGRWYTRDSGHWVYYRHEPPELYRYRSVRVAPPARRVA
                     PRVRVERREYRYSAPPVERNTRARRYHDGGPRRPGP"
     gene            2767..4365
                     /locus_tag="VFQ35_13330"
     CDS             2767..4365
                     /locus_tag="VFQ35_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9931674.1"
                     /translation="MREANVTSNRYLRVAAALGLAACLASAPAQAEERSTKLQVEARC
                     SQCFIDLDEKPRNFDAVYNAWFSHDTREPKYFIAALEQFAVLGAGTAYYWIRPSLNKV
                     DWDFPSPTTRLQYLEAAFDNNKFKTNQVLHPVAGSAYYALARENDLSPLASAAYAAGS
                     SAFFEFVLEWLEKVSYNDLIVTPSAGIPTGEFFFQLSRYLNSTSGAHDAGDYVAEYSL
                     GLPAKLHGDPKGLHSRERMPSDSLGYSSAYWHRFHLAFGLGLTSSGSDTLVFERFSGE
                     GQLFAMPGMLRPGSFATTFGEGNFSEARLHYSFGMRDLEADAAFQTTFAGYYYQDFAT
                     DEDGELRGRAAMVGGSMGYRYHDSQPANGIDQWSFVDLFGPRAQAWLAFGGAVLRVGA
                     DVHYDFGAVYALAYPQYTRVYPRDETKSVLKEQGYQFVQGGSARAEASLTYRSLELGV
                     RRGYGRYYSIQGADRFEDEIRHQVLGTDDISETDFWMRAAAPRPLPLELSIDYNANRH
                     INRLPPFSANRSERRMTFSLGFRL"
BASE COUNT          813 a         1444 c         1376 g          823 t
ORIGIN      
        1 accggggctc gggatccgct tcgcccactc gaccgcttcc tcgagcgact tcacctgcca
       61 gatccagaag ccagccacga gctccttggt ctctgtgaac gggccatcga tgatgctgcg
      121 cttgccggaa gaaccgatga cgacgcgctt gccacgcgag gtggggtgca agccttcgcc
      181 ggcgagcatc acacccgctt tcacgagctc ctcgttgaac ctgcccatgg ccgcgagcgc
      241 ctcggtgctc ggcatctgcc ccgcctcgga ctccttgttt gctttcacca atacaatgac
      301 tcgcatcgct ctcgctcctg tttggagacg gtcccgatga gcgagatgct cacctcgatg
      361 gccgccttac aatcgagcga cgaaccagcc ttcctcggat cgacacgggc ctcgaaatat
      421 ttcggtgctc gacagattca aagaaaatac gttgcgagtt caacaggtta ggcggctttt
      481 cggcaagccg tgacggctcg ggagagccgc tgaaccgcct cgtcgatgcg gtcctcgggg
      541 atgttgcaag gcgagatcct catcgcgcag ggcccggggg agtcctcgtc gacgcggaac
      601 atccgcccgg gatcgaagct ggtgccgaag tcggtcgcga gttcgagcaa cttcatttcg
      661 tcgagacctg gctcgttact ctggatgaac agagtgaagc cgccttcggg ttcgacgaac
      721 gaaaagcctg gaagccagcg ccgaacactc cgcatcaacc gagtgacgcg acacacgtac
      781 gcctcccggg ctcgcttcaa atgcgcggac caatcgagac gggtgaggac gcgctcggcc
      841 aacgcttgcg tcaagctcgg tgcttgcaag tcggcgtctc gctttgcttc gagcagcgcg
      901 ccgcgaaacc cgggtggagc gatgagccag ccgacacgca ggccagggca cagcgtcttc
      961 gacagcgtac ccacgtgaaa gacgcggtcc ggcgcatctg ccaagagcgg gcgttcgggc
     1021 ataccgtcgt aacgcagctc ggcgtacgct tcgtccgcga tgagtgggac gccgctcgca
     1081 aggagctgct gccggcgccg cgaatcgagc ccgagcccga gcgggttgct cgccccgggg
     1141 atcacgtagc gcgcgacgac ggggccctcg gtcacgagcc gcacctcggc gtgtcgaaac
     1201 aggtcgatgg cgccgggata gctgagctcg tcggcagcga tgcgcgcgcg ggatagcccg
     1261 agcgccgaga ccaagagcga cagcgcttgt tgagctccgt tcgtgatgag cacgtcgctc
     1321 gcacgaaccg gggctccgcg cgctcgaagc ccctgggcga cccactcgcg cagtacctcg
     1381 ctgccttcgg gccacgtgta ctgcagcgcc tcgtcgcgtg cgccgtcgtt cagctgctcc
     1441 gccgcgccga acaactcgtc acgcggaaac aacgaagcga gcggcaaccc acccgcgagc
     1501 gacagtgctt gttcttgctt ggctgcttct cgatgaagac gctcgattcg gaagtccatc
     1561 gtcgttcgtt gctcaataaa atgacgcggc cggaaaatcg atcggcacgc tcaggacgtc
     1621 gttgccgtaa atcgtgaagg tgcccaagtc cacctcggtc gtgcgatctg ccccgctcgg
     1681 gtcgagcagc acggcgattc ccgagtaagt ccccggcgcg agatcgatcg acaccgaaaa
     1741 cgcgcggcaa ctctgggtcg tctcgtacga ccaatcgccg cgatcgatca cgacgtcgat
     1801 cgcgtcggcg tcgccgagtg tgcagagcga gctatctttg tagccatgaa tggtccagtc
     1861 gacgaccacc gttccgtccg aggagtgatg atcgctctcc gaaacgaaga agcagccagg
     1921 aagagctagc gcgctcaaaa gaggtatcaa gcgtctcgac atcgccaaat tctccgtaat
     1981 cgaccgttca cgttgtgacg cagattcacg gtcccgggcg ccgtgggccg ccgtcgtggt
     2041 agcgacgcgc tcgcgtattg cgctccaccg gcggcgccga gtaccggtac tcgcgccgct
     2101 ccacgcgcac ccgaggcgca acgcggcgag ctggcggagc aacgcgaacc gagcggtacc
     2161 ggtagagctc cggcggttcg tggcggtagt aaacccaatg accgctgtcg cgcgtgtacc
     2221 agcgaccgtc tacgaggtaa acggtgcgcc cttcgtagta gtaactcggg tacgtctcga
     2281 cataagccgg aggggcttct gcaacgacgt aaccctgtgt tcgcgcataa caaccagagc
     2341 tcgtggcgcc ggcagcgagc agcaccgcga gcttgagcca aggggagttg aagaaggaca
     2401 cgatgagaga cgttcgaagc aaggcgtatg ccgctcggaa caggtggcgc tgcgcattgg
     2461 cgctggttcc ctctcgtgtc tcgcgccctc catcgcctcg ctgaggcggc gttgcgcgcc
     2521 gcgaacgagc gaacaccccc gaacgccgtc atctccgcgg gatgttccgc ttggcgccga
     2581 acgcgatgca cccgttcgcg tgcagcgagc gcaagttttg cgtccccgcc cattcttcac
     2641 tcgcgggatg gatagcaaaa acagtcgaat atcgagggaa atcgcgtgtt gcgacatttg
     2701 gcacgcgtgc tgcagcttgg atgttcagcc ttgtcattcg cagccgattc tcgccctagg
     2761 cgcgtcgtgc gcgaggcaaa cgtaacgtcg aaccgctacc tccgagtcgc tgctgcgctc
     2821 ggtttggccg cgtgtttagc gagcgcgcct gcccaagccg aagagcgctc taccaagctt
     2881 caagtcgaag ctcgctgcag ccaatgcttc atcgatctcg acgagaagcc gcgcaacttc
     2941 gacgccgttt acaatgcctg gttctcgcac gacacccgag agccgaagta cttcatcgcc
     3001 gcgctcgagc agtttgcggt gctcggcgca ggcaccgcct attattggat ccggccgagc
     3061 ctcaacaaag tcgattggga ctttccgagt ccgacgacgc gcttgcaata cctggaagcg
     3121 gcgttcgata acaacaaatt caagaccaac caggtgcttc atccagtggc tggaagcgcc
     3181 tattacgccc tcgctcggga gaacgatctg tctccgttgg cgagcgcggc ctacgcggcg
     3241 gggagctcgg cgttcttcga attcgtcctc gaatggctcg agaaggtgag ttacaacgat
     3301 ctcatcgtca ctccttcggc gggtatcccg acgggcgagt tcttttttca gttgtcgcgc
     3361 tacttgaaca gcacgagcgg cgcgcacgac gccggcgact acgtggccga gtatagcctc
     3421 gggttgccgg cgaagttgca cggggacccc aagggtcttc acagccgcga gcgcatgccg
     3481 agcgactcgc tgggctatag ctccgcttac tggcatcgct ttcacctggc cttcgggctc
     3541 ggcctcacga gctccggctc cgatactctg gtgttcgagc gattctcggg tgaggggcag
     3601 ctcttcgcga tgccgggcat gctacggccg ggtagcttcg caaccacctt cggggaggga
     3661 aatttcagcg aagcacggct gcattacagc tttgggatgc gtgatctgga agccgatgcg
     3721 gcgtttcaga ccaccttcgc cggttactac tatcaggact tcgcaacgga cgaagacggt
     3781 gagcttcgcg gtcgcgccgc catggtgggc ggaagtatgg gctaccgcta tcacgactcg
     3841 cagcccgcga acgggatcga ccagtggtca ttcgtcgact tgttcggccc gcgcgcgcag
     3901 gcgtggctag cgttcggcgg tgcggtcttg cgcgtcggcg ccgatgttca ctacgacttc
     3961 ggcgcggtct acgcgctcgc gtatccccag tacacgaggg tctacccgcg cgatgagacc
     4021 aagtcggtct tgaaagagca gggctaccaa ttcgtgcagg gtggctccgc gcgtgcggag
     4081 gcatcgctca cttatcggtc actcgagctc ggggttcgcc gcggctacgg tcgctactac
     4141 tccattcaag gcgccgaccg gttcgaggac gagatcaggc accaagtgct cggcaccgac
     4201 gacatctccg agaccgactt ctggatgcgc gccgccgcgc cgcgtccgct cccgctcgag
     4261 ctcagcatcg actacaatgc gaaccgtcac atcaatcgct tgccgccgtt ctccgcgaat
     4321 cgcagcgaac gtcgcatgac cttctcgctc ggattccgcc tgtgaggtcc ccatgctccc
     4381 ctcacgctca ctccgtcttg gttcggtcgt cgccctttcc ctcggctgcg cgccggtcgc
     4441 gatgatgcgg ccaccg
//
LOCUS       DASTKS010000643         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_994361, whole genome shotgun sequence.
ACCESSION   DASTKS010000643 DASTKS010000000
VERSION     DASTKS010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994361"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="VFQ35_13335"
     CDS             complement(<1..1871)
                     /locus_tag="VFQ35_13335"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET9931675.1"
                     /translation="MRALFLRAQAERQRVVALVLVAIVYFGIARLSLLLAFEGTNASP
                     VWPPSGFALAILLVLRRGLWPGVASGAFVANAVTFITNGAPLGWKVLLASTVIALGNA
                     TEATLAEAVLDRIVSPKRSPLARALLFAATTGAASAVAAAIGATTVLRGGFAPAAMAS
                     SIFVTWWVGDWVGMLLTTPLLLSILPLEREVIRTGKALPALIACGAAISIWLVARSSA
                     SLGSNELNRALLWLQAGIGAAAIIGLGFGAARSAEQPKPEALALHVDEQTLSLAPALA
                     GLGVLAASLALWRSVLRDHEVALFEATHAASEGVTAAIESALRSDARAVERLAARWVR
                     RGALDEGEWRDSARAFIADFHGFEAFAWVSSENSVGWVESRASATSIALTPAELSQAR
                     SAPGRAIASFRSRSADHEPVLIFAVALSGDSPGLIAGAVRLREVFAEATAHLSSDFEV
                     RVTGPFEPAVGRIGAIGPGRAMAQSATVRGSPTGLRVEVRASPRKARQLRSALPEVIL
                     GGGSVFSAMVAALLFLAQEVRSRERKARSDGEHLMKVNRELAEERKRAEAAAQAKAEF
                     LANMSHELRTPMSAVVGLSALLRDTALSETQREYVNDLELSSNLLRQLIDDILDLSKL
                     "
     gene            complement(2047..>2558)
                     /locus_tag="VFQ35_13340"
     CDS             complement(2047..>2558)
                     /locus_tag="VFQ35_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET9931676.1"
                     /translation="LLNRGVSNGIIAPKDYTNPFNTFCFIPDEKAPATDFNKQVDQAR
                     TNGGWRIVLVHGFVGGTDGAYQPVEFDQFASAVTYAKSLGDVWIDTMVAVGAYWRGQK
                     ALSQAQMTTSGDSTTYTWTLPPHFPPGRTLRVTVDGGTLSQNGAPLAWDEHGYYEISL
                     DALSLTISP"
BASE COUNT          450 a          870 c          839 g          399 t
ORIGIN      
        1 agcttcgaaa ggtccaagat gtcgtcgatg agctgccgca agaggttcga cgaaagctcg
       61 aggtcgttca cgtattcgcg ctgcgtttcg ctgagcgcgg tgtcgcgcag cagcgcgctg
      121 agcccgacga ccgcgctcat cggggtgcga agctcgtggc tcatgttggc caaaaactcc
      181 gccttcgctt gggccgccgc ttcggcgcgc ttgcgctcct cggcgagctc tcggttcacc
      241 ttcatgaggt gttcaccgtc gctgcgcgct ttgcgttcac ggctccgcac ctcttgggcc
      301 aagaacaaga gcgcggcgac catcgcggag aatacgctgc cccctcccaa gatgacctcg
      361 ggcagggccg agcgcagttg cctcgccttg cggggagacg cgcgcacctc gacgcgtagc
      421 ccagtggggg agcctcgcac cgtcgcgctt tgagccattg cccgccccgg gccaatcgca
      481 ccgatgcgtc ccaccgcggg ctcgaacggt cccgtcacgc gcacctcgaa gtcggatgag
      541 agatgggccg tggcctcggc gaatacttct ctcaagcgaa ccgcgcccgc gatgaggcca
      601 ggcgaatcgc cgctcagcgc gacggcgaag atcaagactg gctcgtgatc cgcgctgcgg
      661 ctgcgaaacg acgcgatcgc gcggccggga gcgcttctcg cctggctgag ctctgctggc
      721 gtgagcgcga tgctcgtcgc gctggcgcgc gactcgaccc agcccacgct gttctcggag
      781 ctgacccaag cgaacgcctc gaagccgtgg aagtctgcga tgaaggcccg cgcgctgtcg
      841 cgccattcgc cttcgtcgag cgccccccgc cggacccagc gcgcagcaag gcgctcgacg
      901 gcgcgcgcgt cgcttctcag cgcgctctcg atcgcggcgg tgacgccctc gctcgcggcg
      961 tgagttgctt cgaacagcgc aacctcgtga tcgcgcagaa ccgagcgcca gagcgcgagc
     1021 gaggccgcga gcactccgag cccggcgagc gccggggcca aagacaaggt ctgctcgtcg
     1081 acgtggagcg ccaaggcttc cggctttggc tgctcggcag accgagctgc tccgaagccg
     1141 agaccgatga tcgccgcggc tccgatgcca gcctgcaacc agaggagcgc tcgattcagc
     1201 tcgttgctgc cgagcgacgc cgacgagcgc gccacgagcc agatcgaaat ggccgcgcca
     1261 cacgcaatca acgctggcag cgccttcccc gtgcgaatca cctcgcgctc gagcggcaag
     1321 atgctcaaga ggagcggcgt cgtgagcagc attcccaccc aatctccaac ccaccaagta
     1381 acgaagatcg agctcgccat cgccgcgggc gcaaatccac cgcgtaacac ggtggtagcg
     1441 ccgatcgcgg cggctacggc gcttgccgcg ccggtcgttg ccgcgaacag caaagcgcgc
     1501 gcgagcggcg agcgcttcgg gctgacgatc cggtcgagca ccgcttcggc caaggtggcc
     1561 tcggtcgcat tgccgagcgc gatcacggtg gaggcgagca gcaccttcca accgagcgga
     1621 gccccgttcg tgatgaacgt cacggcgttg gctacgaacg cgccactcgc gacgcctggc
     1681 cagagccccc tccggagcac taggaggatg gcgagcgcga agccggacgg aggccaaact
     1741 ggcgaagcgt tggtaccttc gaaggcgagc agcagggata ggcgcgcgat cccgaagtac
     1801 acgatcgcga cgagcaccag cgccacgact ctctggcgct cagcctgcgc gcggaggaac
     1861 aacgctcgca tcgctatcac cgatatatcg gagtggccgc gttggcgcta cttacggcct
     1921 ggcgacgacg attaccgcca tccatccgaa cggtgtcccg cccgcgacgg tcaccgagac
     1981 taccccggtg accggcgacc attcagcagg cgacgcacgc gagacgtttg ccgcgttcga
     2041 aggctctcag ggggagatgg tcaacgaaag cgcgtcgagc gaaatttcgt agtagccgtg
     2101 ttcgtcccac gccagcggag cgccgttctg gctcaaagta ccgccgtcga cggtgacgcg
     2161 cagagttcgg cccggcggga aatgcggcgg cagagtccag gtgtacgtgg tcgagtcgcc
     2221 gctcgtcgtc atttgcgctt gcgatagcgc cttctgcccg cgccagtagg cgcctaccgc
     2281 gaccatggtg tcgatccaaa cgtcgcccaa cgacttcgcg taagtgacgg cggacgcgaa
     2341 ctgatcgaac tccaccggtt ggtaagcgcc gtcggtccct ccgacgaatc cgtgcaccag
     2401 gacgatacgc catccgccgt tcgttcgcgc ctggtcgacc tgcttgttga agtcggtggc
     2461 gggcgccttc tcgtccggaa tgaagcaaaa cgtgttgaac ggattggtgt agtccttggg
     2521 cgcgatgatc ccgttcgaga cgccgcggtt caggagga
//
LOCUS       DASTKS010000644         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_325691, whole genome shotgun sequence.
ACCESSION   DASTKS010000644 DASTKS010000000
VERSION     DASTKS010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325691"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VFQ35_13345"
     CDS             complement(<1..1213)
                     /locus_tag="VFQ35_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931677.1"
                     /translation="MTDPKPLPNVERDTKPWSPERPFQRVKSPSFTPAGAIEAKRLSG
                     DGHPRGTRRFEDTPFVVPKITIFGQAVQGALDTVLNDKIFERGVRMTPNWVRTLGDLK
                     NGPAEERAAEHEIYGKLLRSFQTWTDVPAFQWHRWYDWNFHVQTVPEFDWLRGEANRI
                     ELGKPKTAPGEPPLEQFCEGTMECEWDAGSIGPKPGPMFTDLNKQTKSEWIWPMAGDW
                     VWIVGRSIYDGGHEFRRKQGGVETGPRLSRSELHPCKAMAMVRKLALPIGGASHRIPV
                     TQFAFFASKLGGYIDFDTLAPLGDDYEFLVDLPLRPDDFQPVTHNIGRTPEFPVNRLV
                     LRHPEPIAEFDFAPYQTAFGALSVDSLKSRFKPEVSFEVPDPAKPEQVQARIRMPLKK
                     LAAETKAGSYGV"
     gene            1373..2401
                     /locus_tag="VFQ35_13350"
     CDS             1373..2401
                     /locus_tag="VFQ35_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931678.1"
                     /translation="MLARAPNGEQVLALDRGNLRLVNVRNASSAWQTPLPSWNEYSRC
                     NAFFSPRGDYFVLGEEHLVGDTKKLYSVWQTASGHRVSANFEHAFASATLDWGIAVSD
                     DGRWFATNRVRESTDLTVFDLGTQAATVLAVHGAADPARFSADSKLLFVRDEVFALTD
                     EGWKSAARFPGAVDAVWAGQRLAVATPGGVDIWERSGVVHVDYPFAFSQNQNKDPKTN
                     VFLRASERFLAICERAEDPSLLVYDLDRHAQAFARRGLGTIGDVTFRDNRPVVLAFRN
                     DFNTFVLMLDPTNGNVVREKSFGAYGSGGPPVSPHNFAFSPRLLPGGRFIELQYADSL
                     ASRFESLD"
     gene            2461..4110
                     /locus_tag="VFQ35_13355"
     CDS             2461..4110
                     /locus_tag="VFQ35_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET9931679.1"
                     /translation="MVDSEVEVHPEATFAVAVAPGDPADDSVFNSLRPGILSIACFRL
                     NDPHFDFDSSFILPATREALVQLGEMRADYADSPASIFGHADPVGTDTYNKTLSGNRA
                     RAFHALLTRDVQAWEALYNDHSWGLKSTQRILKFLRDEAAAKEASEASADDATAPSDT
                     DPDASGDADPDTSGDADPTDFEVDGKNTKEWKAAVKKFQGDESLHVDGDAGPETRKRL
                     YELYMAALCVDLAGNEFKFEPSDFLGKGKDKKGKAAFQGCSEFNPILLLSKSDDDTLE
                     KEERNSMNAPNRRVVLYFFPPGLEIDVDKWPCPRASEGSAGCKKRFWSDHERRRKQDP
                     ELRRSYARQPALELEGTRDTFACRFYDRLARHSPCEAGFEEWLVQLILPEPADKPLAE
                     HKRAAGATFKATFASGDKPAEGEADANGLIRIRARRETDQVTVELKVPLEDDGSAPNE
                     ASGSNDSAGAGDEQTSASTEKRRFATVRLTLQGGGLKELHDEENADAARKDRLLNLGF
                     GPTARANWDKDPKVLQKAQDDAAKQEGGAVDDDKLRQLYGS"
     gene            4160..>4462
                     /locus_tag="VFQ35_13360"
     CDS             4160..>4462
                     /locus_tag="VFQ35_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931680.1"
                     /translation="MEFGRRWKIRTSFGWVVLTLRTPAESEPKRDAAARERERLARSY
                     LLYEVAGSSENPRAPLRTLQAFLQGNASALPEYGTPEVESDVELSALNTLLETELAA"
BASE COUNT          828 a         1424 c         1444 g          766 t
ORIGIN      
        1 ttacgccgta agagccagcc ttggtctcgg ccgcgagttt cttcaaaggc atgcgtatgc
       61 gcgcctgcac ctgctcgggc ttcgccggat cgggcacctc gaacgacacc tcgggcttga
      121 agcggctctt caacgagtcg accgagagcg cgccgaacgc ggtttggtag ggcgcgaagt
      181 cgaactccgc gatcggctcg gggtgccgca agaccaagcg attgaccgga aactccggcg
      241 ttcgcccgat gttgtgagtc accggctgga agtcgtccgg gcggagcggc aaatcgacga
      301 ggaactcgta gtcgtcgccg aggggggcga gcgtatcgaa gtcgatgtac cccccgagct
      361 tgctcgcgaa aaacgcgaat tgagtcaccg gaatgcggtg cgacgctcct ccgatcggca
      421 gcgccagctt tcgcaccatg gccatcgcct tacaaggatg gagctccgag cgcgaaagcc
      481 gcggaccggt ttcgacacca ccttgcttcc gcctgaactc gtgcccgccg tcgtagatcg
      541 atcgcccgac gatccaaacc caatcgccgg ccatcggcca gatccactcg gacttggttt
      601 gcttgttcag gtcggtgaac atcgggcccg gcttcggacc gatcgagccg gcgtcccact
      661 cgcactccat ggtgccttcg caaaactgct cgagcggcgg ctcgccggga gcggtcttgg
      721 gcttgccgag ctcgatgcga ttcgcctcgc cgcgcaacca gtcgaactcc ggcacggtct
      781 ggacgtggaa gttccagtcg taccagcggt gccattggaa cgccggcacg tcggtccaag
      841 tttggaagct tcgcagcagc tttccgtaga tctcgtgctc tgccgctcgt tcctcggcgg
      901 gcccgttctt caaatcgccg agcgtgcgaa cccagttcgg cgtcatacgt acgccgcgct
      961 cgaagatctt gtcgttcaat accgtgtcga gcgcgccctg cacggcttga ccgaagatgg
     1021 tgatcttcgg caccacgaac ggcgtgtctt cgaagcgccg agtgccgcgc gggtggccgt
     1081 ctccgctcag gcgttttgcc tcaatcgcgc cggccggtgt gaaggagggc gatttgacgc
     1141 gttgaaaggg tcgttctggc gaccacggct tcgtgtctcg ttcgacgttc ggtaaaggtt
     1201 tgggatcggt catcgctcat ccccaccggt ggacggaatc ggggcgcatg ttaccttacc
     1261 gacgatgtcg agggtgaggc tatccgctgc gctgcccgtc tcgctccttc ggcgcgcact
     1321 ttcctttgcg gccctcaccc tgagtgcgtg cgccgggaat aaggtgcaag gagtgttggc
     1381 ccgcgcaccg aacggcgagc aggtgctggc gctcgaccgc ggcaatttgc gcctcgtcaa
     1441 cgtgcgcaac gcgagctcgg catggcaaac gccgctcccg tcgtggaacg aatacagccg
     1501 ctgcaacgca ttcttttcgc cgcgcggcga ctacttcgtt ctcggcgagg agcatctggt
     1561 cggggacacc aaaaagctct actcggtatg gcaaaccgcg agcggccacc gcgtgtcggc
     1621 caatttcgag cacgccttcg ccagcgcgac gctcgactgg ggcatcgccg tttcggacga
     1681 tgggcgctgg ttcgcgacga accgggtacg cgagagcacc gacctcacgg ttttcgactt
     1741 gggtacgcag gcggcgaccg tgctcgccgt gcacggcgcg gctgacccgg cgcgcttttc
     1801 agccgattcc aagctgctct tcgtgcggga cgaagtgttc gcgctgaccg acgagggatg
     1861 gaagtcggcg gcgcgctttc caggcgcggt cgacgcagtc tgggccgggc agcggctcgc
     1921 ggtcgcgacg ccgggcggcg tcgatatctg ggagcgatcc ggcgtcgtgc acgtcgacta
     1981 cccgtttgct ttcagccaaa accaaaacaa ggacccgaag accaacgtct ttctgcgcgc
     2041 gagcgagcgg ttcctcgcca tttgcgagcg cgccgaagat ccttcgctgc tcgtctacga
     2101 tctcgatcgc cacgctcagg ctttcgcgcg acgcggcctg ggtacgattg gtgacgtcac
     2161 gttccgcgac aatcggcctg tcgttctcgc gttccgaaac gacttcaaca ccttcgtgct
     2221 catgctcgac ccaaccaacg gcaacgtcgt gagggagaag agctttggcg cgtacgggag
     2281 cggcgggccc ccggtgtccc cgcacaactt cgctttctcg ccgcggctct tacctggagg
     2341 ccgcttcata gagctccaat atgccgatag cctcgcgagt cgcttcgagt cgctcgactg
     2401 aaggtgcgcg ccgcttgccg gtgcacgctc gggcttgtat tactcatttc agggttttgc
     2461 gtggtcgata gtgaggtcga ggtacatccc gaggcgacgt tcgctgtcgc ggtagcgccc
     2521 ggagatccgg cggacgactc cgtcttcaat tcattgaggc ccgggatcct cagcatcgct
     2581 tgctttcgcc tgaacgatcc ccacttcgac ttcgattcgt cgttcatcct ccccgcgacg
     2641 cgagaggcgc tcgtgcagct cggcgaaatg cgcgccgatt acgccgactc gcccgcgtcg
     2701 atcttcggcc acgctgatcc ggttgggacc gacacctaca acaagacgct cagcggcaac
     2761 cgggcgcgcg cgttccacgc gttgctgacc cgagacgtgc aagcgtggga agccctatac
     2821 aacgaccaca gctgggggtt gaagagcacg cagcgcatcc tcaagttctt gcgcgacgaa
     2881 gccgctgcga aggaagcgag cgaggcctca gccgacgatg ccacggcgcc gagcgacacg
     2941 gatcccgacg cgagcggcga cgcggatccc gacacgagcg gcgacgcgga cccgaccgat
     3001 ttcgaggtcg acggcaagaa caccaaagaa tggaaggcag cggtcaagaa gttccaaggc
     3061 gacgagagcc tgcacgtcga cggagacgcc gggcccgaga cgcgcaagcg cctttacgag
     3121 ctctacatgg cggcgctctg cgtggacctc gccggcaacg agttcaagtt cgagcccagc
     3181 gactttctcg gcaagggcaa ggacaaaaag gggaaagccg cttttcaggg ctgcagcgag
     3241 ttcaacccca tcctgctgct ttctaagagc gacgacgaca cgctcgagaa ggaagagcgg
     3301 aactcgatga acgcgccgaa ccggcgcgta gtgctctatt tcttcccgcc cggcttggag
     3361 atcgacgtcg acaagtggcc gtgtccgcgc gcaagtgaag gttctgccgg ctgcaagaag
     3421 cgcttttggt cggaccacga gcggcgcaga aagcaagatc ccgagctccg gcgcagctac
     3481 gcgcgccagc ccgcgctcga actcgaagga acgcgcgata cgttcgcgtg ccggttctac
     3541 gaccgattgg cgcggcactc gccgtgcgag gccggcttcg aagagtggct ggtccagctg
     3601 attctaccgg agccggcaga caagccgctc gccgagcaca agcgcgcagc gggcgcgact
     3661 ttcaaagcga cgttcgcgag cggcgacaag cctgccgagg gcgaagccga cgccaatggc
     3721 ctgattcgca ttcgggcacg ccgcgaaacc gaccaagtga cggtcgagtt gaaggtcccg
     3781 ctcgaagacg acggcagcgc gccgaacgaa gcgtcgggga gcaacgacag cgcgggagca
     3841 ggcgacgagc agactagcgc ttctaccgaa aagcgacgct tcgcgacggt ccgtctcacc
     3901 ctacagggcg gcgggctcaa ggagctgcac gacgaggaga acgccgatgc ggctcgcaaa
     3961 gatcgcttgt tgaacctggg gttcggtcca actgcccgcg ccaactggga caaagatccg
     4021 aaggttttgc agaaggcgca agacgacgcc gccaaacaag agggcggcgc ggtggacgac
     4081 gacaagctcc gtcagctcta cggcagctga cgcggcgatc gaatttgccg cagactgaag
     4141 ccgcactaac gtggggaaca tggagtttgg gagacgttgg aagattcgaa ccagtttcgg
     4201 ctgggtcgtt ctcaccctgc gtaccccagc cgagtcggag ccgaagcgcg atgcggccgc
     4261 gcgcgaacgg gagcgactcg cccggtcgta cctgctgtac gaggtcgcag ggagctccga
     4321 gaatccgcga gcgccgttgc ggacgttgca agcgttcttg caagggaacg cgagcgccct
     4381 gccggagtac ggcacgcccg aagtggagag cgacgtcgag ctcagcgcgc tgaacacgct
     4441 gctcgaaacc gagctcgccg cc
//
LOCUS       DASTKS010000645         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1218837, whole genome shotgun sequence.
ACCESSION   DASTKS010000645 DASTKS010000000
VERSION     DASTKS010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218837"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFQ35_13365"
     CDS             <1..930
                     /locus_tag="VFQ35_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9931681.1"
                     /translation="GMLLAVAMADQHTFDELWLYSQKWVNGNGLMNWYINAAGTQVLG
                     SGAATDADEDIAWSLVMADRQWGGKGSLDKPYIELAKAQIDRIWRTEVAHDQNEMLLP
                     GDTWGSNPLFNPSYFAPNQYRIFGAVTGKTADWNKVIDTGYTIIEKSLNATSKNATNG
                     LVPAWCGADGMPKAPPSGGATNYQYDSARTPYRIGLDFCFNGEERAKNYLGKISSFFA
                     GVGAGSIVDGYDLDGKPHPDPDSPSGSPQSAVFVGCAAVGAMHDAKYQSFIDDAYTRV
                     ATGQLLARSRYYNLSWTALNLLMLTGNFAEYAN"
     gene            complement(950..2740)
                     /locus_tag="VFQ35_13370"
     CDS             complement(950..2740)
                     /locus_tag="VFQ35_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931682.1"
                     /translation="MLKQAQQFALSRQLETESIVAREEALDTMDPLVRLLVRRRIAKL
                     ERRAELLTLHADPQHRARLAYLLGDGREVALPDDVDSVRTQAEQSRPPDSPGRKGPWL
                     SALCIAAILASAGAVFAARSILKPKRPETTPAAQLLGQSLPRFVVAVLRDDTPLQTEL
                     MTQMTGPLGAELLGVRSANELAALLRSTQALHAGKGSPPTPLLEAFQNRAVAFDKSLA
                     DATLPFFVDADTATLEQWPEPVLQSYYVQRESSATNGATRVRVLQLWRLDKLNVRHRV
                     LGYTRATTPAAIVLLDQVESDLVRFTLPAASSGGTLELVDANTRTRGEAWVSDVETQA
                     AKVVGSYYKGLGALSDSAAKLAELLGRRKELLRKWDASLRGQGLELNPPTGLFADADL
                     TKVLGLRVPRAEREEWDAIEDELHERMPSFEALRDRFVLGVERHEVQHRLDYQRGLIP
                     VPASLCGWLGLENPLDAPLGGLAARARDETSAYLAELTRNEDSPLLELVILSNFVLDQ
                     QARNAPYTYAALAVYAALAKALGIDVDAVLGRSISRQRFARLAVELWSRPPDELREAA
                     RRAYLAEFGVELPSVKRENGHENARYRPEP"
     gene            complement(2749..>3227)
                     /locus_tag="VFQ35_13375"
     CDS             complement(2749..>3227)
                     /locus_tag="VFQ35_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931683.1"
                     /translation="KRGWGGLMNTTNDSIDDIAAITPTPGVKLAGGLTILSGFISGVL
                     ALQAGTLLGARGVYRLSEPLFALLGLACLVSGFRLTRMRAGSARWATVAAALACVSGL
                     VWFVMTLQAGVFVLMALGLVPLASIATIAGLASLKPVERAAAARRRLREQGLDSGL"
BASE COUNT          577 a         1057 c         1060 g          533 t
ORIGIN      
        1 ggaatgttgc tcgccgtcgc gatggcggac cagcacacct tcgacgagct ctggctctac
       61 tcgcagaagt gggtgaacgg gaacggcttg atgaactggt acatcaacgc tgcgggcact
      121 caggtgctcg gcagtggcgc cgccaccgat gcagatgaag acatcgcctg gtcgctcgtg
      181 atggccgacc gacagtgggg cgggaagggc agcctcgaca agccctacat cgagcttgcc
      241 aaggcccaaa tcgaccgcat ttggcgcacc gaggtcgccc acgatcaaaa cgagatgctc
      301 ttgccagggg acacttgggg cagcaaccct ctcttcaacc cttcgtactt tgcgccgaac
      361 caatatcgca ttttcggcgc cgtcaccggc aagaccgctg actggaacaa ggtcatcgac
      421 accggctaca ccatcatcga gaagagcctg aacgcgacca gcaagaacgc gacgaacggg
      481 ctcgtccccg cctggtgcgg cgctgacggc atgccgaaag cgccgccttc gggcggtgcg
      541 acgaactacc agtacgactc ggcgcgcacg ccttaccgca tcggcctcga cttttgcttc
      601 aacggcgagg agcgcgcgaa gaactacctc ggcaagatca gctcgttctt cgccggcgtc
      661 ggagcaggct ctatcgtcga cggctacgac ttggacggta agccacaccc cgatcccgat
      721 tcgccgagcg gcagcccaca gtcggccgtg ttcgtcggct gcgccgccgt cggcgcgatg
      781 cacgacgcca agtaccagtc gttcatcgac gacgcttaca cgcgagttgc taccgggcag
      841 ctcctcgctc gcagccgcta ctacaacctg agttggaccg cgctgaatct cttgatgctc
      901 accggcaact tcgccgagta cgcaaactga gcagcggcgg ctcgtaggtt cacggctctg
      961 gccgataacg cgcgttttcg tgcccgttct cgcgcttcac gctgggtagc tcgacgccga
     1021 actcggccag gtaggcgcgc cgggccgctt cgcgcagctc gtcgggaggc cgcgaccaga
     1081 gctcgaccgc caagcgcgcg aagcgctgtc gcgagatcga gcggccgagc acggcgtcga
     1141 catcgatgcc gagcgctttg gcgagggctg cgtacacggc gagcgccgcg taagtatacg
     1201 gtgcgttgcg cgcttgttga tcgagtacga agttcgaaag gatcacgagc tcgagcagcg
     1261 gcgagtcctc gtttcgcgta agctctgcga ggtaggcgga agtctcgtct cgtgctcgcg
     1321 cggcgagccc gcccaggggg gcgtcgagcg ggttttcgag cccgagccag ccacaaagcg
     1381 acgccggtac ggggatgagc ccacgctggt agtctagtcg gtgctgtacc tcatggcgtt
     1441 cgacgccgag gacgaagcga tcgcgcaacg cttcgaagct cggcattcgc tcgtggagct
     1501 cgtcttcgat ggcgtcccac tcttcgcgct ccgcgcgcgg cacgcgtagc ccgagcacct
     1561 tcgtcaggtc cgcgtcggcg aagagcccag tcggcggatt cagctcgagg ccctggccgc
     1621 gcagcgaagc gtcccactta cgcaggagct ccttccggcg accgagcagc tcggcgagct
     1681 tcgccgcgga atcggagagg gcaccgagac ccttgtagta acttccgacg accttggccg
     1741 cttgtgtctc gacgtcgctt acccaggctt caccccgcgt gcgcgtgttc gcgtcgacga
     1801 gctccagcgt tccgcccgaa gatgccgcgg gcagcgtgaa acggactagg tccgactcga
     1861 cttggtcgag aagcacgatc gcggccgggg tagtcgcccg agtgtagccg agcacgcggt
     1921 gtcgcacgtt gagcttgtcg agccgccaga gttgaaggac gcggactcgc gtcgctccgt
     1981 tcgtcgcgct cgactcgcgc tgcacgtaat agctctgcag cactggctcg ggccactgct
     2041 cgagagttgc ggtgtccgca tccacgaaga aggggagagt cgcgtctgcg agcgacttgt
     2101 cgaatgcgac ggctcggttc tggaaggctt cgagtagcgg cgtgggaggg ctgcctttgc
     2161 ctgcgtggag ggcttgcgtg ctccgcagca gggccgcaag ctcgttcgcc gaacgcacgc
     2221 cgagcagctc ggcaccgagc ggccccgtca tctgggtcat cagctccgtt tgaagcgggg
     2281 tgtcgtcgcg taacacggcg accacgaagc gcggcaggct ctgtccgagg agctgggcgg
     2341 cgggcgttgt ttcggggcgc ttcggcttca ggattgagcg cgccgcgaac acggcgccgg
     2401 cgctggccag gatcgcggcg atgcagagcg cgctgagcca cggccccttt cgtcccggcg
     2461 aatccggcgg tctcgactgc tcggcttggg tgcgaactga gtcgacgtcg tccggaagcg
     2521 caacctcgcg cccatctccg agcaggtagg cgagccgagc gcgatgctga ggatccgcgt
     2581 gcagcgtgag aagctcggcg cggcgctcga gcttcgcgat acgtcgccgc accaaaaggc
     2641 gcacgagtgg gtccatcgtg tcgagcgcct cttcgcgggc gacgatcgac tccgtctcga
     2701 gttgtcgaga gagcgcgaac tgttgtgcct gcttcagcat cgcggagctc aaagaccgga
     2761 atcgaggccc tgctccctca gtcgtcgacg cgccgcggcg gctcgctcga cgggcttcag
     2821 actggcgagt ccagcgatgg ttgcaatgct ggcgagcggt accaatccga gcgccatcaa
     2881 cacgaacact cccgcctgga gcgtcatcac gaaccaaacg agccccgaca cacaggcgag
     2941 agcggcggct acggtcgccc aacgcgccga gccggcgcgc atccgcgtaa gacggaaccc
     3001 actcacgagg cacgcgagcc cgagcagagc gaagagcggc tccgacaatc gatagacgcc
     3061 ccgggcgccg agcaaggttc ctgcctgcag agccaaaacc cccgagatga atccgctcag
     3121 gatcgtgagg ccacccgcta acttgacccc cggagtcggt gtgatggcgg cgatatcgtc
     3181 aatcgagtcg ttcgtagtgt tcatgaggcc tccccaacca cgcttta
//
LOCUS       DASTKS010000646         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_549938, whole genome shotgun sequence.
ACCESSION   DASTKS010000646 DASTKS010000000
VERSION     DASTKS010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549938"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFQ35_13380"
     CDS             complement(<1..219)
                     /locus_tag="VFQ35_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931684.1"
                     /translation="MSFEKPPIPSGASGPPPALRRLLRAIPWLVALFASLAVVLGLRD
                     TPRRHALETALPSVTAPKGPTARVCLTLL"
     gene            263..1462
                     /locus_tag="VFQ35_13385"
     CDS             263..1462
                     /locus_tag="VFQ35_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9931685.1"
                     /translation="MRSFWAIRACALGLLVNALGVGCSNHDHAPPPSPAASGAAGKGS
                     GSGGGITIGGDFSGPPTCGGQTLQAITHPPVLYFVIDRSGSMGEAFDSFGTTKYAASL
                     TAIGRLLDAVGSRVRYGAAIYPATDIDSSCEAGFQMFPPTLGDALADIPAGGRGPILL
                     ELMRRLSAYLPGGATPTSATLNALRSHIAEYAAGGDTTQVVLVTDGAPNCNFDVTCDS
                     SSCIPDIEAAITQQGSVCGRGRSCCDRSLGANAGGNCIDGDASEQAVRALFDANVRTF
                     VVGLPGSEAYAKTLDRLADAGGTARDGTPRYYAVSDQEALSTALLEIGSAVTIPCEVE
                     LQAAPANPALVNVYFDGQVVPRDGENGWDYGGDTALTLAGEACKSLQAGAVHEVQIAY
                     GCETIVR"
     gene            1513..1800
                     /locus_tag="VFQ35_13390"
     CDS             1513..1800
                     /locus_tag="VFQ35_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9931686.1"
                     /translation="MKSPSPELDSLRQAIDQVDRRILDLLEERVRLVLAVGDYKRKNS
                     LPVYDPERERSLLERLSGAARPPLDGDTVRRVFERLIDESRRIEQHHVQQR"
     gene            2036..2407
                     /locus_tag="VFQ35_13395"
     CDS             2036..2407
                     /locus_tag="VFQ35_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9931687.1"
                     /translation="MRLAEQVRQLRVQAGMTQAELAARAGVTVETVARLERVLRGRSS
                     ANSNPSLETLARLSSALGVEVSDLLSAPSRPRPRDDRLFVILRSATPATRRRVLRVAE
                     ALVREDASEAKPESRTRRRPS"
     gene            complement(2434..>3617)
                     /locus_tag="VFQ35_13400"
     CDS             complement(2434..>3617)
                     /locus_tag="VFQ35_13400"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9931688.1"
                     /translation="SGSRFEPHGLGGISHFHEHMLHRGTTAHPTAHALALAFEELGSE
                     LGAATYVDHTTIGAGAPLENLDRVIGLLGELVQNPVWSAIETERAIVREEILESLNER
                     GESVDADEALIALAFPQHGLGRPIAGTIQALDRFDEPALSRFHAAHYHGGGLVVSVAG
                     PLDANAVAATLESAFGGLANGAPPEDAPPKSLAGPCFRYVSDSGTQTALRVAFRAPAE
                     VDKLEPATELLLRTLDDGMSTRLYHQVCDERGLAYDVSASYEAFHDAGLFTIAGESAH
                     GSAERLLRAFFEVVSDLRENGPNDAELEKAQRRFAWQMRSVLDDPGELAAFLGLGELT
                     GTVLTPASRQKELADVSSDLVREAARRVFQPSELAVVAVGDLTTTQRRALERALKSFG
                     A"
BASE COUNT          594 a         1174 c         1258 g          591 t
ORIGIN      
        1 gagcagcgtg aggcagacgc gcgcggtcgg tcctttcgga gccgttacgc tcgggagcgc
       61 tgtttcgagc gcgtggcgcc gcggcgtatc gcgcagcccg agcaccaccg cgagcgaagc
      121 gaacagcgcg acgagccacg gaattgctcg gagcagcctt cgaagcgcgg ggggcgggcc
      181 ggaagcccct gacgggattg gcggcttttc gaaactcacc gagcgccgac catagcgcgc
      241 ctctgcctgc tatcttttcg cgatgcgttc attttgggca attcgcgcct gcgcgctggg
      301 attgctcgtg aacgcgctcg gggttggctg ctccaatcac gatcacgcgc ccccgccgtc
      361 gcccgcggcg agtggtgcgg caggcaaagg ctccggtagc ggcggcggta tcaccatcgg
      421 cggcgacttc agcgggccac cgacctgcgg gggacaaacg ctgcaagcga tcacgcatcc
      481 gccggtgctc tacttcgtca tcgatcgctc gggcagcatg ggcgaggcgt tcgactcgtt
      541 cggcaccacc aagtacgcgg cttccctgac cgcgattgga cgcctgctcg acgcggtcgg
      601 atcgcgcgtc cgctacggtg ccgcgatcta cccggcgacg gacatcgaca gcagctgcga
      661 ggcgggattc cagatgttcc cgccaacgct cggcgatgcg ctcgccgaca tccccgcggg
      721 cggtcgcggc ccgatcttgc tcgagctgat gcggcgtctt tcggcgtact tgccgggggg
      781 tgcgacgccg acctcggcca cgctcaacgc tttgcgctcg cacatcgcgg agtacgcggc
      841 gggcggcgat accactcagg tcgtcctcgt gacggacggc gcgccaaact gcaacttcga
      901 cgtcacgtgt gactcgagct cctgcatccc cgacatcgaa gccgccatca cgcagcaggg
      961 ctcggtgtgt gggcggggaa ggagctgctg cgatcgcagc ctgggcgcga acgcgggcgg
     1021 caactgcatc gacggggacg cgagcgagca ggccgtgcgc gctctcttcg acgcgaacgt
     1081 acgcacgttc gtagtcggtc tgcccggcag cgaggcctac gcgaagacgc tcgaccgttt
     1141 ggcggacgcg ggcggcaccg cccgggacgg gacgccccgc tactacgccg tttccgacca
     1201 ggaagcgctg tcgacggcgc tcctcgagat cggcagcgcc gtgacaatcc cctgcgaggt
     1261 cgagctccag gcggccccgg cgaatccagc gctcgtcaac gtgtacttcg acgggcaggt
     1321 cgtaccgcga gacggcgaaa atggctggga ttacggcggg gacacggcgc tcacgctggc
     1381 tggcgaagct tgcaagagcc tgcaggcagg ggccgtgcac gaagttcaga tcgcgtacgg
     1441 ctgtgaaacc atcgtgcgct gatgcgctcg ctgttgtagc ctctcgtgcg acaagttgag
     1501 cgagcgcccc acatgaaaag tccctccccc gagctcgatt cgctgcggca ggcgatcgac
     1561 caggtggacc gccgtatcct ggacctgttg gaagagcggg tgcgcttggt gctcgccgtc
     1621 ggcgactaca aacgaaaaaa tagtctcccg gtttacgatc ccgagagaga acgatcgctg
     1681 ctcgagcgac tatccggcgc ggcgcgtccc ccactcgatg gggatacggt gcgccgtgtg
     1741 ttcgagcggc tgatcgacga gtcgcgccgc atcgagcagc accacgtgca gcaacgttag
     1801 gggtgtctcg ggctatcggc ccgctggcca gcgttgctga gtagtcagtt cgaactttgc
     1861 gtttcgggcc gagcacgctc tccgcgtggc cctcgtaacc gtgcagggtg gtgaactacg
     1921 acgggcgtcg aagtccaggc gcagagttct aatgacaagg cgaagaaaag tcgtaagtta
     1981 cgtttcgctc aagggctggg gagtctgaga gctagctttt gtgaatacca ttgcgatgcg
     2041 gctggcggag caagtccggc agctacgggt acaagccgga atgacccaag ccgagcttgc
     2101 tgctcgagcg ggcgtgacag ttgagacggt tgctcgcctc gagcgcgtat tacgtgggcg
     2161 atcttcagcc aattcgaacc cgtcgctcga gacgctggcg cgattgagct cggcgctcgg
     2221 cgtcgaggtc tccgatttgc tgtctgcgcc gagccgaccg aggccgcgag acgaccggtt
     2281 gttcgtgatt ttgcgtagcg cgacgcccgc gacgcgacgc cgcgtgctgc gagtagccga
     2341 ggcgctcgtg cgcgaggacg cgagcgaggc gaaaccggag agccgcacgc gccggcgccc
     2401 cagctgagcc gagaagcctc actcgccgaa gcgctacgcg ccgaagcttt tcagggcgcg
     2461 ctcgagggcg cgccgctgcg tcgtcgtgag atcacccacc gcaaccacgg cgagctcact
     2521 cggctggaag acgcggcgcg cggcttctcg cacgaggtcc gacgagacgt cggcgagctc
     2581 tttctgtcgc gacgctgggg tcagcaccgt tccggtgagc tcccccaaac ccaggaaggc
     2641 ggcgagctcg ccggggtcat ccagcaccga gcgcatttgc caagcgaagc ggcgttgcgc
     2701 cttctcgagc tcggcgtcgt ttgggccgtt ctcgcggagg tcgctcacta cctcgaagaa
     2761 ggcccgcagc agtcgctcgg ccgagccgtg agcggactca cccgcgatcg tgaataggcc
     2821 agcgtcgtgg aacgcctcgt agcttgcgga gacgtcgtaa gcgaggccgc gctcgtcgca
     2881 cacttggtga tagaggcgcg tcgacattcc gtcgtcgagc gtccgcaaga gcagctcggt
     2941 tgcgggctcc agcttgtcca cttcagccgg agctcgaaag gcgacgcgga gagccgtctg
     3001 agtgccggag tcgctcacgt aacggaaaca aggccccgcg agcgacttcg gtggggcgtc
     3061 ttcgggggga gcgccgttcg caaggccacc gaatgcgctc tcgagcgtcg cggccaccgc
     3121 gttggcgtcg agcggccccg cgacgctgac cacgaggccg ccgccgtgat agtgcgccgc
     3181 gtgaaaccga ctgagcgccg gttcatcgaa gcgatcgagc gcttggatcg tgccggcaat
     3241 ggggcgcccg agtccgtgct gggggaaggc gagcgcgatg agggcttcgt cggcgtcgac
     3301 cgactcgccg cgctcgttca acgactccaa gatctcttcg cgcacgatgg cgcgctcggt
     3361 ctcgatcgcg ctccaaaccg ggttttgaac cagctcgccg agcagcccga tgactcggtc
     3421 gaggttctcg agcggcgcgc ccgccccgat ggtcgtgtgg tccacgtagg tcgcggcccc
     3481 gagctcgctg ccgagctcct cgaaggcgag cgcgagcgca tgcgcggtcg ggtgcgcggt
     3541 ggtgccccgg tgcaacatgt gctcgtggaa atgactgatg ccgcccagcc cgtgcggctc
     3601 gaagcgcgag ccgctga
//
LOCUS       DASTKS010000647         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_995109, whole genome shotgun sequence.
ACCESSION   DASTKS010000647 DASTKS010000000
VERSION     DASTKS010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995109"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..797
                     /locus_tag="VFQ35_13405"
     CDS             207..797
                     /locus_tag="VFQ35_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931689.1"
                     /translation="MSVSVPPLAEFLRWLAEMPPVFHAEPEGFVEGSVRTRAVVADLF
                     ETLFGEAPNDSVLAGLDATDTGKTERNRLRWILAAAHLVWHPSFRSAPPARGALEKLF
                     FQELPSLAGTVDVAQLAHDSERREELIRRTLRLFERRLPGETENEASDRLKQVDSIER
                     RRVLLAAADRERRAREVREAMARKAAEEAAAKVSRE"
     gene            825..3167
                     /locus_tag="VFQ35_13410"
     CDS             825..3167
                     /locus_tag="VFQ35_13410"
                     /inference="COORDINATES: protein motif:HMM:NF040095.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET9931690.1"
                     /translation="MTISVLITQCLQRDFVDLLEPYQPLPNRLHVGHQEALRLLGADV
                     QSGPVSQLMAWARGRAESEMEIIHIRDWHDPNDPRQRDHLARFGDHCVRGTRGAELVF
                     GLDEAQRANEIVVDAIALNDFEGTTLNEHLTQIQRRAGGEPLRVGVVGVWTEAKVSFL
                     LYDLKTRCGIEQLATCSALTASASRAQHFNALEQLGRILGVECFDSVGEFVSWLAPGA
                     ALALPPVPARFGGQVIVSDGQAELSQADRDLVTYLHRDSARIVLEPLSGGFSGAAVYR
                     ASSTDAFGHQHAPSVVKLGPQRAIARERVNFERVEEVLGNNAPNVRAFVDLGERAGIR
                     YSYAAMGQGTVRTFKKLFESGYPSPKLDAILHAVFDEILGPLYAAARYEKLPILAHYA
                     FSPDWASSVRKTVTALVGERANARELDFSGGYRASNVCLFYEEFLRSGVIETATDFHY
                     ASVVHGDLNGANILVDGRENVWVIDYFHTGRGHVLKDLAKLENDILYIYTPIPTREAL
                     AEALRVTSALRAVEDLRQPLPERIEGVASEPLLRAWAAIRTLREIVARLCREDRHPLQ
                     MDVALLRFAVHTLSFDESSPLQKEWALAAACGFADDIVRAHAADRELRVDWLDSARLP
                     KGRFGLTLCPGRRDRGRDLTSDLQTLTRQKVTRLLCLVTDEELEWAGVSDIARAAAAH
                     GIELRHHALMDQGAPSVEDAEALVAWCREACERGEAVVVHCMGGLGRSGVIAACLLAS
                     AGASGEEAITTVRSARGPRAIESRQQERFVRHFAETRARA"
     gene            3164..>3798
                     /locus_tag="VFQ35_13415"
     CDS             3164..>3798
                     /locus_tag="VFQ35_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931691.1"
                     /translation="MNLLTRLREHPDAPRWNHSASDRLNPEDLMALDRLRDDLATKRG
                     ARDAEPSPAIVQRIAALREKVPSFRKRLHGLSDIAAEWHRIPTMSREDIAISVQELVP
                     DDADLENLIVYRTAGTSGHALLVPHHARAAASYQPLIEHALARYGVSPQFDPDTVACF
                     LVGAQARTVTYPTILSAWAGAGFAKLNLRPGDWPREHSAHRYFEAMSPRLLT"
BASE COUNT          644 a         1403 c         1114 g          637 t
ORIGIN      
        1 tgggacgttg gtcttgaacc tgcctgacgt agccagatgg cgggcgcggg acccgctcga
       61 gaaaatcggc taccgtgtgc atgccgtgcg tgcctgggag gacctcatcg tgtttgctcg
      121 agctttcgtg cgcgaaaact acggaggcaa ctcgtgagcg cggcccagcg gaaccggcgc
      181 tttctcgaag ccgagggaga agagccgtga gcgtgagcgt tcccccgctc gccgagtttt
      241 tgcgctggct cgcggagatg ccgcccgtct tccacgccga gccagaaggg ttcgtcgaag
      301 gcagcgtgcg cacgcgagcc gtcgttgccg atttgttcga gacgctgttt ggtgaagccc
      361 cgaacgattc agttcttgcc gggctcgatg cgaccgacac cgggaagacc gagcgcaatc
      421 ggctgcgctg gattctggcc gccgcgcacc tcgtgtggca cccctcgttt cgcagcgccc
      481 cgcccgcgcg cggtgcgctc gaaaagctct tctttcaaga gctccccagc ctagccggca
      541 ccgtcgacgt cgcgcagctc gcgcacgaca gcgaacgccg cgaagagctc attcgtcgta
      601 cgctacgcct cttcgagcgc cggcttccgg gtgaaaccga aaacgaagcc agcgaccgct
      661 tgaagcaagt cgactccatc gagcgccgcc gcgtgctcct tgcggccgcc gaccgcgagc
      721 gcagggcccg cgaagtgcgc gaagccatgg cgcgcaaggc cgccgaagaa gccgccgcca
      781 aagtgagccg cgagtagatc cgtcagcggc gaacaggtca cctcatgacg atttcggtcc
      841 tcatcaccca atgcctgcag cgcgacttcg tcgacctgct ggagccctat cagccgctgc
      901 cgaatcgctt gcatgtcggc caccaagagg cgctgcgcct gctcggtgcg gacgttcaga
      961 gcggcccggt ctcccaattg atggcctggg ctcgcggtcg cgccgaaagc gaaatggaga
     1021 tcatccacat tcgcgactgg cacgatccca acgacccacg ccagcgcgac cacctggcgc
     1081 gcttcgggga ccactgtgtg cgcggcaccc gcggcgcgga gctcgtcttt ggcctcgacg
     1141 aagcgcagcg tgccaacgaa atcgtcgtcg acgccatcgc gctcaacgat ttcgaaggca
     1201 ccaccctcaa cgaacacctc acgcagatcc aacgacgcgc cggtggtgag cccctgcgcg
     1261 tcggcgtggt cggcgtctgg accgaagcca aagtcagctt cctgctttac gacctcaaga
     1321 cccgctgcgg catcgaacag cttgccactt gcagcgcgct caccgccagc gcctcgcgcg
     1381 cccaacactt caacgctctc gagcagctcg gccgcatctt gggcgtcgaa tgcttcgact
     1441 cggttggtga attcgtgtcc tggctcgctc cgggcgccgc cctcgccttg ccgcccgtgc
     1501 ccgcgcgttt tggcggtcag gtgatcgtct cggacgggca ggcggagctc agccaggccg
     1561 atcgcgatct cgtcacgtac ctgcaccgcg acagcgcgcg catcgtgctc gagcccctct
     1621 cggggggatt ctcgggcgcc gccgtgtacc gcgcctccag caccgacgcc tttggacacc
     1681 aacacgcgcc gtccgtggtc aaactcggac cccagcgcgc catcgcccgc gagcgcgtca
     1741 acttcgaacg cgtcgaagag gtgctgggca acaacgcacc caacgtgcgc gccttcgtcg
     1801 acttgggtga gcgcgctggc atccgttact cctacgccgc gatgggccaa ggcaccgtcc
     1861 gcaccttcaa gaagctcttc gaaagcggct accccagccc caagctcgac gcgattttgc
     1921 acgccgtttt cgacgaaatc ttggggccgc tctacgctgc cgcccgctac gaaaagctcc
     1981 ccatcctcgc tcattacgcg ttttcgcccg actgggcctc gtcggttcgc aaaaccgtca
     2041 ccgcgctcgt cggcgagcgc gccaacgccc gtgagctcga cttcagcggc ggctaccgcg
     2101 cgagcaacgt ctgcctcttc tacgaagaat tcctgcgcag cggcgtcatc gagaccgcca
     2161 ccgacttcca ctacgcgtcg gtcgtccacg gcgacttgaa cggcgccaac atcctcgtcg
     2221 acggccgcga aaacgtgtgg gtcatcgact actttcacac cggtcgcggc cacgtcctca
     2281 aggatctcgc caagctcgag aacgacatcc tctacatcta cacgcctatc cccacgcgcg
     2341 aagccttggc cgaagccttg cgcgttacca gcgccctccg cgccgtcgaa gatctgcgcc
     2401 aaccgctgcc cgaacggatc gaaggcgtcg cgagcgagcc gttgctgcgc gcttgggcgg
     2461 ccattcgcac cctgcgcgaa atcgtcgccc gcctctgccg tgaagatcgc catcccctgc
     2521 agatggatgt cgcactgctg cgctttgccg tccacactct gagcttcgac gaatcgtctc
     2581 cccttcagaa agagtgggca cttgccgccg cctgcggttt tgccgacgac atcgtccgcg
     2641 ctcacgccgc cgatcgcgaa ctccgcgtcg attggctcga cagcgcgcgc ctcccgaaag
     2701 gccgctttgg cctcactctc tgccccggtc gtcgcgatcg cggccgcgac ttgaccagcg
     2761 atctccaaac cctgactcgc caaaaagtca ctcgcctgct ttgcctcgtc accgatgagg
     2821 aactcgagtg ggcgggagtc agcgacatcg ctcgagccgc ggccgctcat ggcatcgaac
     2881 ttcgccatca cgccctgatg gaccaaggcg cccccagcgt cgaagatgcc gaagccctcg
     2941 tcgcctggtg tcgcgaggcc tgcgagcgcg gggaagccgt cgtcgtccac tgcatgggcg
     3001 gcctcggtcg ctcgggcgtg atcgcggcct gcctgctcgc cagcgcgggc gcctctggtg
     3061 aagaagcgat caccaccgtg cgcagcgcgc ggggcccgcg tgcgatcgaa agccgacagc
     3121 aggaacgatt cgtgcgccat ttcgccgaga cgcgggctcg agcatgaacc tactcacacg
     3181 cctccgcgag caccccgacg cgccgcgctg gaatcacagc gcgagcgatc gcctcaaccc
     3241 cgaagacctc atggcgctcg atcgcctgcg cgacgacttg gccaccaagc gcggcgcgcg
     3301 cgacgccgag cccagccctg ccatcgttca acgcatcgcc gccctgcgcg aaaaggtccc
     3361 ctcttttcgc aagcgcctgc acggcctctc agacattgcc gccgagtggc accgcatccc
     3421 caccatgtcg cgcgaagaca tcgccatctc cgtgcaggag ctcgtgcccg acgacgccga
     3481 tctcgaaaac ctcatcgtgt atcgcaccgc cggaaccagc ggccacgccc tgctcgtgcc
     3541 tcaccacgcc cgcgcggccg cttcttatca gcctttgatt gaacatgcgc tcgcgcggta
     3601 cggcgtcagc ccgcaattcg accccgacac ggtcgcttgt ttcttggtgg gcgctcaagc
     3661 tcgcaccgta acttatccca ccatcctctc cgcgtgggcc ggtgccggct tcgccaaact
     3721 caacttacgc cccggcgact ggcctcgcga acacagcgcg catcgctact tcgaagccat
     3781 gtccccgcgc ctcctcac
//
LOCUS       DASTKS010000648         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1219345, whole genome shotgun sequence.
ACCESSION   DASTKS010000648 DASTKS010000000
VERSION     DASTKS010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219345"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VFQ35_13420"
     CDS             <1..919
                     /locus_tag="VFQ35_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931692.1"
                     /translation="TDYAFCEELARRKVYVSLQLDALSNPALRVLRGGGDQRTAKEKA
                     LKNLERAGVRTTIVSTVAKGVNDDAIGDCIRLLYERDFILSLTFQPAAYTGYGGEHFA
                     PHNPLDVITIPDIVRAAATQTGGMLTESDFLPLPCSHPSCFGLTYLLKTEDGFVPFPR
                     FLELEKYLELLANRGTIRPAAEFEGAIKSAIDEMWTSAGQVPDPAKILRALKRAIHLM
                     YPEDRALELEERLHIGESLVKTVFIHAFMDVHTFEVDRIKKCCTHYAMPDGRLMPGCA
                     YNLLYRERDARYVGPVGQTKIWGRNEGPS"
     gene            916..1656
                     /locus_tag="VFQ35_13425"
     CDS             916..1656
                     /locus_tag="VFQ35_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931693.1"
                     /translation="MKLLNGKRTLITGGARGLGRAIALAFAKHGARVAFTYAKNEEAA
                     ERTRRELAELGVDALSYRVDAADSAGTEAMLSAVEKAWDGLDVLVNNAAITQNLPLAL
                     LDEEDFDRVMRVNVKGPFITSRAALRGMIRRKSGVIVNIGSLAGARMIEAPIHYTTSK
                     AAIIGMTEALAKEVARHGIRVVCLAPGLLEDGLGRNLPDHRLVDYLHHCALGRVGKFE
                     EVAEMCALLASDRSSYVSGETLVMDGGV"
     gene            1643..2377
                     /locus_tag="VFQ35_13430"
     CDS             1643..2377
                     /locus_tag="VFQ35_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931694.1"
                     /translation="MAASESLPGVIVLGGSGAVGGAICRALVADGARVGFTYFKNADA
                     AAALERELPGSIAKRVDLESVAELTRTLTELRADLGGVCGLVHAATLTSAARDPKFEK
                     LDEADEASFDRLFAVNVRSAYFACREFARDFRGNIVLLGSLDGAKSLPSPVAYAASKG
                     ALVAMARALSKELGSRGVRVNVVAPGVLERGSSELVPAELRAEYLKHCGLKRFGKVEE
                     VARLVSYLTLFNTYVTGQTLVVDGAL"
     gene            2377..2898
                     /locus_tag="VFQ35_13435"
     CDS             2377..2898
                     /locus_tag="VFQ35_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931695.1"
                     /translation="MWKVGVAALLCYAIHAGSHVLAGRPAEVLWACHVATLLVGAGAL
                     SENATLVGIGVSWLAFGNPLWILDLMTGGEFLLTSPLTHVGGLVLGCIALRQLPPPAK
                     TIWWKAALAFLALLVLTRALTPPSANVNLAFAVAPGWERAFPSYPLYFLLLLGAGTAT
                     FFVTELGMRRVLS"
     gene            2895..>3366
                     /locus_tag="VFQ35_13440"
     CDS             2895..>3366
                     /locus_tag="VFQ35_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9931696.1"
                     /translation="MNDGSIVDLYARHVNPAFVRLLGTFGYGRVFVRARDCSLFDDQG
                     REYLDMLAAFGACNLGHNPPRLVEKMKSFLDDEAMNLIHVGPQPHAARLASELAAVLK
                     PPLEVSLFSNSGGEAVEAAIKLARVATGRSAIVYTHGGFHGTGLGTLSILGHPRL"
BASE COUNT          562 a         1124 c         1079 g          601 t
ORIGIN      
        1 gaccgattac gctttctgcg aagagctcgc gcgacgtaag gtgtacgtca gcctgcagct
       61 cgacgcgctg agcaaccccg cgctgcgcgt cctacgcggg ggcggcgacc agcgcaccgc
      121 caaagagaag gcgctgaaaa atctcgaacg cgccggggtt cgcaccacca tcgtctcgac
      181 ggtcgccaag ggcgtcaacg acgacgccat cggcgactgc attcgcctcc tctacgagcg
      241 cgatttcatc ctgtcgctga cgtttcaacc ggcggcgtat accggctacg gcggcgagca
      301 cttcgcgcct cacaatccgc tcgacgtcat cacgatcccg gacatcgtgc gcgcggccgc
      361 gacgcaaacc ggcggcatgc tcaccgaaag cgactttttg ccgctccctt gctcgcaccc
      421 gagctgtttc gggctcactt acctcctgaa gaccgaagac ggcttcgtcc ccttcccgcg
      481 cttcctcgag ctcgagaagt acctcgaact gctcgcaaac cgcggcacga ttcgacccgc
      541 ggccgaattc gagggcgcaa tcaagagcgc catcgacgag atgtggacga gcgccggtca
      601 ggttccggac ccggccaaaa tcctgagagc gctcaagcga gcgattcacc tcatgtaccc
      661 cgaagaccgc gcgctcgaac tcgaggagcg gctgcacatc ggcgagagcc tggtcaaaac
      721 ggtcttcatc cacgccttca tggacgtgca caccttcgag gtggatcgca tcaagaagtg
      781 ctgcacccac tacgcgatgc ccgacgggcg cttgatgccc gggtgtgcgt acaacttgct
      841 ctaccgtgaa cgcgatgcgc gttacgttgg ccccgtcggt caaaccaaga tttggggccg
      901 caacgagggc ccttcgtgaa gctactcaac ggtaagcgca cgttgatcac cggtggagca
      961 cgcggtctcg gccgcgccat cgcgctcgcc tttgccaagc acggcgcgcg cgtcgcgttc
     1021 acctacgcaa agaacgaaga agcggcggag cgcacccggc gcgagctcgc cgagctcggc
     1081 gtggacgccc tgagttaccg cgtcgatgcg gccgacagcg ccggcaccga agcgatgctc
     1141 tccgcggtgg agaaggcgtg ggacgggctc gacgtcttgg tgaacaacgc cgccatcacc
     1201 caaaacctgc cgctcgccct gctcgacgaa gaagacttcg atcgcgtgat gcgcgtcaac
     1261 gtaaaagggc cgttcatcac gagccgcgcg gcgttgcgcg gcatgatccg ccgcaaatcc
     1321 ggggtgatcg tcaacatcgg ctcgctcgcg ggcgcgcgca tgatcgaagc gcccatccac
     1381 tatacgacga gcaaggccgc catcatcggc atgaccgaag cgctcgccaa ggaggtcgcg
     1441 cgtcacggga ttcgcgtcgt gtgcctcgcg ccggggcttt tggaagacgg cctcggccgc
     1501 aacctgccgg accatcgctt ggtcgactac ttgcaccact gcgcgctcgg gagggtcggt
     1561 aaattcgagg aagtcgcgga gatgtgcgcg cttctcgctt cggaccggag ctcgtacgtc
     1621 tccggagaaa cgctggtgat ggatggcggc gtctgaatca ctcccaggcg tgatcgtcct
     1681 cggagggagc ggcgcggtgg gaggcgcgat ctgtcgcgcg ctcgtagccg acggagcccg
     1741 tgtcgggttc acgtacttca agaacgcgga cgctgccgcc gctctcgagc gcgaacttcc
     1801 gggcagcatc gccaaacgcg tcgacctcga gtccgtcgcg gaactcactc gtaccctcac
     1861 ggagctacgc gccgacctcg ggggcgtgtg cgggttggtt cacgccgcga ccctgacttc
     1921 ggcggcgcgc gaccccaagt tcgagaagct cgacgaagct gacgaggcgt cgttcgaccg
     1981 tctgtttgcg gtcaacgttc gcagcgccta tttcgcttgc cgcgaattcg cccgcgactt
     2041 tcgcggaaac atcgtgctcc tcgggtcgct cgacggggcc aagtcgttgc cttcgccggt
     2101 cgcctacgcc gcgagcaagg gggcgctggt agcgatggcc cgcgccctct cgaaggagct
     2161 aggcagccgc ggagtgcgcg tgaacgtagt cgcgccgggc gtcctcgagc ggggctcgag
     2221 cgagctcgtg ccggccgaac ttcgcgccga gtacctgaag cattgcgggc tgaagcgctt
     2281 cggaaaagtc gaagaggtgg ctcggctcgt atcgtacctg accctcttca acacgtacgt
     2341 caccggtcaa acactcgtgg tcgacggagc gctctgatgt ggaaggtcgg tgtcgccgcg
     2401 ctgctctgct acgcgatcca cgcgggctcg cacgtgctcg ccggccgccc cgcggaggtg
     2461 ctttgggcat gccacgtcgc taccctgctc gtcggcgcgg gggcgctcag tgagaacgcg
     2521 acgttggtcg ggattggcgt gtcgtggctc gctttcggca atccactttg gattttggat
     2581 ctgatgacgg ggggagagtt cttgctgacc tcgcctttga cgcacgtagg agggcttgtg
     2641 ctcggttgca tcgcgttgcg gcagctgccc ccgcccgcga aaaccatttg gtggaaggcg
     2701 gcgctcgcgt tcctcgcctt gctcgttctc acgcgtgcgc tgacgccgcc gagcgcgaac
     2761 gtcaaccttg ccttcgcggt ggcgcccggc tgggagcgcg cttttccgtc gtatccactg
     2821 tatttcttgc tgctgctcgg tgccgggacg gcgacgttct tcgtgaccga gctcggcatg
     2881 cggcgggtgc tctcgtgaac gacggctcca tcgtcgatct ctatgcgcgc cacgtgaacc
     2941 ccgcgttcgt gcggctactc ggcactttcg gctacggccg cgtcttcgtc cgcgctcgcg
     3001 actgcagctt gttcgacgac cagggccgag agtacctcga catgctggcc gcgttcggcg
     3061 cctgcaatct ggggcacaat ccgccgcggc tcgtcgaaaa gatgaagagc ttcctcgacg
     3121 acgaagccat gaacctgatt cacgtcgggc ctcagccgca cgccgcgagg ctcgcgtcgg
     3181 agctcgccgc ggtcttgaag ccaccgctcg aagtcagcct gttttctaac agcgggggcg
     3241 aagcggtgga agctgccatc aagctcgcgc gcgtcgcgac cggacgttcc gcaatcgtgt
     3301 acacgcacgg cggtttccat ggcaccggcc tcggcacact cagcatcctc gggcacccgc
     3361 gcttgc
//
LOCUS       DASTKS010000649         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1889595, whole genome shotgun sequence.
ACCESSION   DASTKS010000649 DASTKS010000000
VERSION     DASTKS010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889595"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFQ35_13445"
     CDS             <1..708
                     /locus_tag="VFQ35_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931697.1"
                     /translation="GEPPAAKPTDSPTTGEPEPPPSPAPPPPREEPPAKPEEAPKPAK
                     APRPVAPSTIRSPTTPADGASPVTRGSADHARLRALLAVDARRNLEVTSYCYGGKVGL
                     DYAGFRFLLEGCGAVVSVSRGTITSGIGDVRVGRAQPIVVLGGVQLGVDLSVAGGANW
                     AVGNSSVVNTHLRRVLMPYADARVGLFAASRARTAFTPLIEVYAGRAVGIVSNADGEA
                     VSATGGWFSGVELGLWL"
     gene            1412..1786
                     /gene="rbfA"
                     /locus_tag="VFQ35_13450"
     CDS             1412..1786
                     /gene="rbfA"
                     /locus_tag="VFQ35_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9931698.1"
                     /translation="MGNFSESKSERRSTRRAGIRAPRLELLFCEELCSLFADEINDAR
                     LSGVRVTRVELSRDGARARVWFDWVEPDFDAREVERALQRASGFLRSRLCEALPLKRS
                     PELSFRRDPACAGQVFEDLETD"
     gene            1786..>2382
                     /locus_tag="VFQ35_13455"
     CDS             1786..>2382
                     /locus_tag="VFQ35_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931699.1"
                     /translation="MSKKNIARTALEGGRRYYNCWYRRRTNAVVRAEERTAFSRLRDR
                     EDVDDILWPKREPVYQDFYDKLAPVFRWLKAQAGRPWSKVRSELTQRFDTRTTAGRHI
                     IFCHVLPAVDPVPRFSRYHPVYVDRYGILRLVRPKKRKGVRAKVHWLSPSTTQWLEGR
                     RIAQRGELLYWFVPTESGAFRQANRLNDAEAEQFREIPE"
BASE COUNT          382 a          711 c          828 g          461 t
ORIGIN      
        1 ggtgagcccc ccgctgccaa gcccaccgac tcgccaacaa ccggcgagcc agagccaccc
       61 ccgagccccg cgcccccgcc tccgcgagag gagccgcccg ccaaaccaga agaagctcca
      121 aagccagcga aggcaccacg accggtggcg ccgagcacga ttcgttcgcc tacgacgccc
      181 gcggacggag cgtctcccgt gacgcgcggc tccgcagacc acgcgcgcct gagagcattg
      241 ctcgccgtcg acgcgaggcg caacctcgaa gtgacatcgt actgttacgg tgggaaggtc
      301 ggcctcgact acgctggttt caggttcttg ctcgaggggt gcggcgccgt ggtgagtgtt
      361 tcgcgtggca ccatcacgag cggcatcggc gacgtgcgcg ttgggcgagc gcagccaatc
      421 gtggttctcg gcggtgttca actcggtgtc gacctcagcg ttgccggcgg cgcgaattgg
      481 gccgtcggca attcgagtgt cgtcaatacg catctacgcc gcgtgctaat gccatatgca
      541 gatgcgcgcg tcgggctctt cgcggcttcg cgcgcgcgca cggccttcac tccgctcatc
      601 gaggtgtatg cagggcgcgc tgtcggcatc gtatccaacg ccgacggtga ggccgtatcc
      661 gcgactggtg gttggttcag cggtgtcgag ctcggccttt ggctgtgagc ggtgtcgagc
      721 tcggcctttg gctgtgagcg gtgtcgagct cggcttgaca gggctgcgcg tgtctgtcaa
      781 atactcgccc acgccgttcg tggcagagaa gggagatgtc gctcatgcac aacttgaata
      841 atcattgcgc agatcgtgcg acgtttccct gcgtagctcg gtcttaagtt cgagctggcg
      901 gttcgccaaa gtcggcgagc ctgcgtcatc tcgagacgct gggctacgta gctagctctg
      961 tgcgggcgcg gttcacgtcg cgtcctattc aagtgggcgg tgtcaacgga accgttcgtt
     1021 cctttccgcc ccgcgcgctc gccttcgggc agcgccgaac ccaataaatc agccaatttg
     1081 ctgcgattcc ggatccgttc cggaacggcc gcgcgctcgt cctcgagcgt gaacggcggc
     1141 gtgtgtggtt gctctcgagc gggatgtcgc atgtcgggtt cgagcccggt gtgacgtcgc
     1201 gttggatctc tcgagtgcga tgtcgcgtgc cgggcttcga gctcggggtg acgtcgcgat
     1261 ggagagtctg atctctcgag cgcgatgtcg cgtgcttgcg aattggacag tgcctcgttc
     1321 tcactcgtga gtgcgcggcg agtcgatcga gctcgaagct cgatcggaaa tgggaggggt
     1381 gtgccttgcg cactcctcag gtggtagccc catgggcaat ttttcagaat cgaagtcaga
     1441 gcgacgctcg acgcgccgtg cggggattcg cgcgccgcgg ctcgagctcc tgttttgcga
     1501 agagttgtgt tcgttgtttg ccgatgaaat caatgatgcg cggctgagcg gtgtgcgtgt
     1561 cacgcgcgtc gagctgtcgc gtgatggagc gcgggctcgg gtctggttcg attgggtcga
     1621 gccggacttc gatgcgcgcg aggtcgagcg tgcgttgcag cgggcgagcg ggttcctacg
     1681 gagccgcttg tgcgaggcgt tgccgctgaa gcgcagccca gagctcagct ttcggagaga
     1741 tccggcgtgt gcggggcagg ttttcgagga tttggagacg gactgatgtc gaagaagaac
     1801 atcgcgcgca ctgccttgga gggcgggcgc agatactaca attgctggta ccggcggcgc
     1861 acgaacgcgg tcgtgcgcgc cgaagagcgg accgcgtttt cgaggctgcg cgatcgcgag
     1921 gacgtcgacg acatcctttg gccgaagcgg gaaccggtat accaagactt ttacgacaag
     1981 ctcgcgcccg tgtttcgttg gctcaaggcg caagcagggc ggccgtggtc gaaggtacgg
     2041 agcgagctca cgcagcgctt cgacacgcgc acgacggcgg ggcggcacat catcttttgc
     2101 catgtgctgc ccgcggtcga cccggtgcca cgcttctcgc gctatcaccc ggtgtacgtc
     2161 gaccgctacg gcatcctgcg gctcgtgcgg ccgaagaagc gcaaaggcgt gcgggccaag
     2221 gtccattggt tgagcccgag tacgacgcaa tggctcgaag ggcggcgcat cgcgcagcgg
     2281 ggcgagctgc tgtattggtt cgttccgacc gagtccgggg cgttccgaca agcgaatcgc
     2341 ctgaacgacg cagaggcaga gcagtttcgt gagatccccg ag
//
LOCUS       DASTKS010000650         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1443482, whole genome shotgun sequence.
ACCESSION   DASTKS010000650 DASTKS010000000
VERSION     DASTKS010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443482"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFQ35_13460"
     CDS             complement(<1..660)
                     /locus_tag="VFQ35_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HET9931700.1"
                     /translation="MIGDGGDTSSGGTIARAGAPGTGGAAGHATSGAGGSATATGGAS
                     ANGGAGGTGGSCPPGKALSGGRQYCSNSKGSVSGGYSYERWASGSGSGCMTVYGKNAA
                     FKATWTNVGDFLARIGLLFDQTKTPAQLGTISADFAETTTGNMGLIYVGIYGWSVSPL
                     REYYIIEDWGSSRPAELASDGSPRTKMGEIVVDGDTYDVWTHTQTNKPAITGDNQTFV
                     QY"
     gene            1306..3522
                     /gene="katG"
                     /locus_tag="VFQ35_13465"
     CDS             1306..3522
                     /gene="katG"
                     /locus_tag="VFQ35_13465"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HET9931701.1"
                     /translation="MTESVSETENPAIASPTPKTTRPRTNRDWWPNQLDLQVLHQHGP
                     KSNPLGRDFDYAKHFAELDVDALKRDLVSAMTNSQPWWPADYGHYGPLFIRMSWHAAG
                     TYRIEDGRGGAGEGAQRFAPLNSWPDNANLDKARRLLWPIKQKYGQKISWSDLLVFAG
                     NVALESMGFKTFGFGFGRPDVWEPPEIFWGPEDTWLGDERYSGERELANPLGAVQMGL
                     IYVNPEGPNGKPDPRAAAHDIRETFARMAMDDEETVALIAGGHTFGKTHGAGDPSRVG
                     PEPEGCPIHHQGLGWKSSHGSGKGKDAITSGLEGAWTPTPIEWDNSFFDTLLAYEWEL
                     AKSPAGAKQWKPKNGAGAGTVPDAHDPSVKHAPMMATTDLALLADPAYEKISRRFHAN
                     PGEFADAFARAWYKLLHRDMGPLSRYLGPWVPPPQLWQDPVPPVDHVLVGAQEVAALK
                     GEILSSGLSTPQLVSVAWRSAASFRGTDKRGGANGARIRLAPQKDWEVNEPAELSKVL
                     EKLEAIQKAFNASRTDQKRVSLADLIVLGGCAAIERAAKAGGHDIVVPFSPGRTDASQ
                     EQTDAHSFAVLEPKGDGFRNYVRAGQKLSPETLLLERANLLTLTAPEMTALIGGMRVL
                     GANFGNSSHGVFTRRAGALTNDFFVNLLDMGTEWQPSPSGGGTYEGRSRETKQVQWTA
                     TAVDLVFGSHSQLRAIAEVYASADAGEKFVRDFVAAWNKVMNLDRFDLARSHTMHT"
     gene            complement(3562..>5212)
                     /locus_tag="VFQ35_13470"
     CDS             complement(3562..>5212)
                     /locus_tag="VFQ35_13470"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9931702.1"
                     /translation="KRAVHVPDRMVELYRGVPSFSHYGAVSYLGVPLLDSQDRIIGQV
                     AVLDDKPMPSEPRNMAIFQIFANRAAAELQRLDAERATKEREAQLRLLVDNAMDAIVD
                     FDDEFQVALMNPAARRIFGYTEPPGQASDVRDLLSKASRAKLEGCVLELTAPSADRSL
                     WLAGGLDALTRDGGHFQAEATLSHYRREHRHKFTLILRDVDERLQAEQRIITLTREAR
                     YLSEELKSFQSFERIVGSSAALLRALRALEQVAPTDTTLLLLGETGTGKELFARAAHA
                     GSKRSEGPLIKLNCGAIPHNLIESELFGHEKGAFTGATQRRDGRFALADGGTLFLDEI
                     GELPLELQPKLLRVLQEGEIEPVGSSRVLKVDVRIVAATNRDLSELVAQGRFREDLFY
                     RLNVFPVTIPPLRERDSDIDELARVFLEKYARRLGRTLAPLSEPTLRKLRRYRWPGNV
                     RELQNVIERGVLVSTSGVFDVDLALPEAAKKAPPVEAREPDDNRVLSMAELERLERKN
                     FQRALAQTRGKVSGPDGAAALLGMNPSTLSSRLKALGIRTK"
BASE COUNT          914 a         1681 c         1693 g          924 t
ORIGIN      
        1 gtactggacg aaagtctggt tatcacccgt gatggcgggt ttgttggtct gggtatgggt
       61 ccagacgtcg taggtgtcgc cgtcgaccac gatctcaccc atcttcgtgc gtggcgaacc
      121 gtccgacgcg agctcggcgg gccgtgagct gccccagtcc tcgatgatgt agtactcccg
      181 cagagggctc accgaccagc cgtagatccc aacgtaaatc aagcccatgt tccctgtcgt
      241 ggtctcggcg aaatcggcgg aaatggtgcc gagttgcgct ggcgtcttcg tttgatcgaa
      301 cagcagcccg atccgcgcca agaaatctcc gacgttcgtc cacgtggcct tgaaggcggc
      361 gttcttgcca tagacggtca tgcatcccga gccgctgccg ctcgcccaac gctcgtaaga
      421 atacccgcca ctcacactgc ccttggagtt cgagcagtac tgcctgccgc cgctcagcgc
      481 cttgccgggg ggacagctgc cacccgttcc tccagcaccg ccgttcgcgc tcgcgcctcc
      541 ggttgcggtg gcgctgccgc ctgcgccgct cgtcgcatgt ccggcagcgc cgcccgtgcc
      601 gggagcaccg gcgcgcgcaa tcgttccgcc cgaactcgta tcaccgccat cgccgatcac
      661 tcctcccgag ctcgtcgttc ccgcggaact cgtaccaccg aggccgctcg tacccgcgct
      721 gctcgcgccg gcggcattcg gcgcgccgcc tgaactcgtg atcgctcccg atgccgcggt
      781 ggccccgccg gcgggggcgc cgcccgagtt cgatggcggt gcgccaccgc tcgaggcgct
      841 cgaattgttc ccgcctccgc taccccctgc ggagcgttgt tcatcactgc agccgagagc
      901 agcgagaaga caggccgcat ggaagctcgg cgaacgcagc cgcgggcgtg ggcgaggcat
      961 catcacggca agatgccctg gtcctcgagc cggaatcaat gtgttgcaat gccactcgag
     1021 gtttgttggg cgactgccac ctcgtcgtta gcccatgtgg agagtttcga gaggagaaac
     1081 gtgcactctc gactgctgaa tgggttcgcg ttggtcgacc ggcgttcacc cacgcgagct
     1141 catcgtcgat acgagcactt cgacggttca tctccggccg gcttgtccag gccttctcgc
     1201 ggccgacgca attcgacaca cggatgatgc tgttttctcg gccgagatcc gctacggaaa
     1261 tcgtggcgtc aacgaacgct agccagcgcg agaggaggac gaaccgtgac tgagagcgtc
     1321 agcgaaaccg agaaccccgc gattgcttcc cccacgccga agacaacgcg cccgcgaacc
     1381 aatcgagact ggtggcccaa tcagctcgat ctgcaggtct tgcaccagca cggcccgaag
     1441 tcgaacccgc tcgggcgaga cttcgattac gcgaagcact tcgcagagct cgacgtcgat
     1501 gcgttgaagc gcgacctcgt gagcgcgatg acgaactcac agccctggtg gcccgcagac
     1561 tacggacact acggacccct cttcatccgc atgagctggc acgccgcggg gacttaccgt
     1621 atcgaggacg gtcgaggcgg cgcgggggag ggggcccaac gattcgctcc gctcaacagc
     1681 tggccagaca atgcgaacct ggacaaggcg cgtcggttac tctggccgat caagcagaag
     1741 tacggccaaa agatttcctg gtcggatctc ctggtgttcg ccgggaacgt ggcgctcgag
     1801 tcgatgggtt tcaagacgtt cggctttggt tttgggcgcc ccgacgtttg ggagcctccg
     1861 gagatctttt gggggcccga agacacctgg ctcggcgacg agcgttacag cggcgagcgc
     1921 gagctcgcga acccgctcgg cgccgtgcag atgggtctca tctacgtgaa ccccgaaggc
     1981 cccaacggaa agcctgatcc gcgggcggca gcgcacgaca tccgcgaaac cttcgcccgg
     2041 atggcgatgg acgatgagga aaccgtcgcg ctcatcgcgg gcgggcatac attcggcaag
     2101 actcacggcg cgggcgatcc gagccgtgtc ggtcccgaac ccgagggctg cccgattcat
     2161 caccagggcc tcggttggaa gagctcgcac ggcagcggta aaggcaaaga cgcaatcacg
     2221 agcggtctcg agggggcttg gacgccaacg cccatcgagt gggacaatag cttcttcgat
     2281 accttgttgg cctacgagtg ggagctcgcg aagagccctg ccggcgccaa gcaatggaag
     2341 ccgaagaacg gcgcgggcgc ggggacggtg cccgacgcgc acgacccttc cgtgaagcac
     2401 gctcccatga tggcgacgac cgatctcgcg ctactcgccg atccagccta cgagaagatc
     2461 tcgcggcgct ttcacgcgaa cccgggcgag ttcgccgatg cctttgcaag agcttggtac
     2521 aaattgcttc accgcgacat ggggcctttg tcacgctacc tcggtccttg ggtcccgccg
     2581 cctcagctct ggcaagatcc ggttccgccc gtggaccacg tgctcgttgg cgcgcaggaa
     2641 gtcgcggccc tcaagggcga gatcctgagc tcggggctct ccacgccgca gctggtatcc
     2701 gttgcgtggc gctccgcggc ttcgttccgc ggcaccgaca agcgcggcgg ggccaacggt
     2761 gcgcgcattc gactcgctcc gcaaaaagat tgggaagtca acgaaccggc ggagctctcg
     2821 aaagtgctcg agaaactcga ggcgatccag aaggccttca acgcctcgcg cacggaccag
     2881 aagcgagtgt cgctcgcgga tctcatcgtc cttggcggct gcgcggcgat cgaacgagcc
     2941 gcgaaagccg gcggacacga catcgtggtt ccgttcagcc cggggcgtac cgacgcatcc
     3001 caagagcaaa cggacgcgca ctctttcgcg gtattggaac ccaagggcga cgggttccga
     3061 aactacgttc gcgcaggtca aaagctctcg ccggaaacgc tgttgctcga acgcgcgaat
     3121 ctgctcacgc tcaccgcgcc cgaaatgacc gcgctcatcg gaggcatgcg agtgcttggc
     3181 gcaaacttcg gcaattcatc tcacggcgtg ttcacgcggc gagccggcgc gctgaccaac
     3241 gacttcttcg tgaatctgtt ggatatgggg accgagtggc agccttcgcc ctcgggcggc
     3301 ggtacttacg agggccgttc gcgggagacg aagcaagtcc aatggacggc tacagcggtg
     3361 gatctggtct ttgggtcgca ctcgcagctc cgtgccatcg ccgaggtgta cgcttccgcc
     3421 gatgcgggag agaagttcgt tcgcgatttc gtcgccgcgt ggaacaaggt catgaacctc
     3481 gaccgcttcg atctcgcgcg ctcgcacacg atgcatacct gaagtcgtcg cggcggcgcg
     3541 cgaggcgtga cgccgcgacc atcacttcgt ccgaatcccg agggccttca gccgcgagct
     3601 cagggtcgac ggattcatgc cgagcagcgc cgccgcgccg tccggtccgg agactttccc
     3661 gcgggtttgc gcgagggcgc gctggaagtt cttgcgctcg agtcgctcga gctctgccat
     3721 cgaaagcaca cgattgtcgt cgggctcgcg cgcctcgacg gggggtgctt tcttcgcggc
     3781 ctcggggagc gcgagatcga catcgaaaac gccgctggtc gaaacgagaa caccgcgctc
     3841 gatcacgttt tgaagctctc gcacgttgcc gggccagcgg tagcggcgca gcttgcgtag
     3901 cgtcggctcg gagagcggcg cgagcgtgcg acccaggcgg cgcgcgtact tttccaggaa
     3961 cacccgcgcc agttcgtcga tatcggagtc gcgttcgcgc aggggcggga tcgtcaccgg
     4021 aaagacgttc agtcggtaga agagatcctc gcggaaacgg ccttgtgcca cgagctcgga
     4081 gagatcgcgg ttggtggcgg ctacgatgcg cacgtcgacc ttgaggacgc gcgagctccc
     4141 cacgggttcg atctcgcctt cttgtaaaac tcgcaggagc ttgggctgga gctcgagtgg
     4201 cagctcgccg atctcgtcga gaaatagcgt tcccccgtcc gccagcgcga agcgcccgtc
     4261 tcggcgttgg gtggcgcccg tgaacgcgcc cttttcgtgt ccgaagagct cactttcgat
     4321 caagttgtga gggatggcgc cgcagttcag cttgatgagc ggaccctcgc tgcgcttact
     4381 tccggcatgc gcggcgcggg cgaagagctc tttgccagtg ccggtttcgc ccaatagcag
     4441 cagcgtggtg tccgtcggcg cgacctgttc gagcgcgcgc agcgctctca agagcgcggc
     4501 agaagacccg acgatgcgct cgaagctctg gaacgacttg agttcctcgc tcaggtagcg
     4561 cgcctcccgc gtcaacgtga tgatacgctg ttcggcctgc aatcgctcgt ccacgtcccg
     4621 caagatcaga gtgaacttgt ggcggtgctc gcgccgatag tgtgacagcg tcgcttcggc
     4681 ctggaagtgg ccgccatcac gggtgagtgc gtcgagccct ccagcgagcc aaaggctccg
     4741 atcggcgctg ggcgcggtga gttcgagcac gcagccttcg agcttagcgc gcgaggcttt
     4801 gctcaacaga tcgcgtacgt cggaggcttg gcccggaggc tcggtgtaac cgaagattcg
     4861 gcgcgcggcg gggttcatca gcgcgacctg gaactcgtcg tcgaagtcga cgatggcgtc
     4921 catagcgttg tcgaccagaa ggcgcagctg agcttcgcgc tccttcgtgg cgcgctcggc
     4981 gtccagtcgc tgcagctccg cggccgcccg gttcgcgaag atctgaaaga ttgccatgtt
     5041 gcgcggctcg ctcggcatgg gcttgtcgtc gagcacggcg acttggccga tgatgcgatc
     5101 ttgcgaatcg agcaaaggta cgccgagata gctgaccgcg ccgtagtgac tgaacgacgg
     5161 aacgccgcgg taaagttcga ccatgcggtc cggtacgtgt acggcgcgct tc
//
LOCUS       DASTKS010000651         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_772244, whole genome shotgun sequence.
ACCESSION   DASTKS010000651 DASTKS010000000
VERSION     DASTKS010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772244"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFQ35_13475"
     CDS             <1..1066
                     /locus_tag="VFQ35_13475"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET9931703.1"
                     /translation="EVKLKQAELSLGDVRLRLAGTVSRDAEHTTVNVHAEVPQAACGA
                     LLAAVPHGMAPMLEALRVEGTLSLRSKVDFDSAHVDATGINLDVSNQCRITGVPLAIS
                     PNRFRDAWWREVKGPDGLPMTIQSGPGSPDWTAYEEISPYLETAILVCEDAGFFAHRG
                     IDYRAIENSIRMNLEAGRFLRGGSTVTMQLAKNLYLSKEKTISRKFQEAVFTHLLEQE
                     LSKHELMELYLNVIEFGPGIYGVRQAARYYFNEEPRDLSLGQALYLASILPAPDTQHF
                     AADGHVKDGWAGYLRKLMQIARKIRRISEEELQAGLAEQVAFRKPSERVGALGVAEDA
                     DDVPDDDERDDGPEAPAAAP"
     gene            complement(1068..1265)
                     /locus_tag="VFQ35_13480"
     CDS             complement(1068..1265)
                     /locus_tag="VFQ35_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931704.1"
                     /translation="MRLVNGYVCRNCADEELAKKGIDPAHPKREFSGSEVYVPPPKTP
                     ELGVNAPEKTGDVGAKLNLYA"
     gene            complement(1354..1869)
                     /locus_tag="VFQ35_13485"
     CDS             complement(1354..1869)
                     /locus_tag="VFQ35_13485"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET9931705.1"
                     /translation="MTNPTDGFLRGDQATPESTDVEFMRLALEEADSAVLHGDVPIGC
                     VVVGADGQVLARDHNRREELGDPTAHAEVLALRKAATVIGHWRLEGATVYSSLEPCPM
                     CAGALVVSRVARLVYGARDLKAGAVDTLFEIGRDTRLNHRFAIESGVLAQDSAARLGA
                     FFAKLRAAGEK"
     gene            complement(1892..2497)
                     /gene="leuD"
                     /locus_tag="VFQ35_13490"
     CDS             complement(1892..2497)
                     /gene="leuD"
                     /locus_tag="VFQ35_13490"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9931706.1"
                     /translation="MALEKILQVSGRGVYVAGNDIDTDRIIPARFMKCVTFDGLGQYL
                     FHDVRFNEDGSKKAHPLNDPRFEGAKILISNSNFGCGSSREHAPQALAKFGIRAVIAE
                     SFAEIFFGNSTTLGMPCVSLSHDDIVKLAAQVEKEPSTEVRIDLQANKVNAGPLSLPF
                     TMKPSARDALMNGVWDPIGQLLDGTQAVKALAQKLPYLHFG"
     gene            complement(2517..>2747)
                     /locus_tag="VFQ35_13495"
     CDS             complement(2517..>2747)
                     /locus_tag="VFQ35_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9931707.1"
                     /translation="FRDAGFEWRQPGCSMCLAMNPDKLNGRELCASSSNRNFKGRQGS
                     PTGRTVLMSPVMVAAAAISGKIADAREVFGAS"
BASE COUNT          480 a          887 c          897 g          483 t
ORIGIN      
        1 cgaagtgaag ctcaagcaag ccgagctctc gctcggagac gtgcggctgc ggctcgccgg
       61 gacggtgtcg cgtgatgccg agcacacgac cgtgaacgta cacgccgagg tcccgcaggc
      121 cgcgtgcggg gcgctgctgg ctgcggtacc gcacgggatg gcgccaatgc tcgaagcgct
      181 gcgcgtcgag ggcacgctgt cgcttcgctc caaggtcgac ttcgacagcg cgcacgtgga
      241 cgcgacgggt atcaacctcg acgtgagtaa ccagtgtcga ataacgggcg ttccactggc
      301 gatttcgcca aatcgcttcc gcgacgcatg gtggcgtgaa gtgaagggac ccgacggcct
      361 accaatgacc attcagagcg gtcccggctc gcccgactgg accgcctacg aggagatttc
      421 gccctacctc gagaccgcga ttttggtctg tgaagatgcc ggattcttcg cgcaccgcgg
      481 catcgactac cgcgcgatcg agaactccat tcgcatgaac ctcgaggcgg gtcgcttttt
      541 gcgcggtggc agcacggtca cgatgcagct cgcgaagaac ctctacctca gcaaggagaa
      601 gacgatctcg cgcaagttcc aggaagccgt gttcacgcac ctgctcgagc aggagctctc
      661 gaagcacgag ctgatggagc tttacctgaa cgtcatcgag tttggtcccg gcatctacgg
      721 tgtgcgacag gcggcgcgct actacttcaa cgaagagccg cgcgacctgt cgctgggcca
      781 ggcgctttac ctcgcctcga tcctgcccgc acccgatacg cagcactttg ccgccgacgg
      841 ccacgtgaag gacggatggg caggctacct tcgcaagttg atgcaaattg cccgcaaaat
      901 ccgcagaatt tccgaagaag agctgcaggc cgggctcgct gagcaggtgg cgttccgcaa
      961 gccgagtgaa cgcgtcggag cgcttggggt cgccgaagat gcggacgacg tgcccgacga
     1021 tgacgagcgc gacgacggac cggaagcgcc ggccgccgcc ccgtgactca ggcgtatagg
     1081 ttcagcttcg cgccgacgtc gccggtcttc tctggtgcgt tcacgccgag ctcgggcgtc
     1141 ttcgggggcg gtacgtacac ttcgcttcca gagaactcgc gcttcgggtg cgccgggtcg
     1201 atgcccttct tcgcgagctc ctcatcggcg cagtttcggc acacgtagcc gtttaccaaa
     1261 cgcatgctca aaaagggcct cgctcggagt gaacggcacc cgccaaaaat cttgaggaga
     1321 aattccgtgg gcgctctcgg gtgcgagcgc cggctacttt tccccagccg cccttagttt
     1381 cgcgaagaac gcgcccaggc gagcggcgct gtcttgcgcc agaacgccgg attctatggc
     1441 gaagcgatgg ttcaaccgcg tgtcgcgacc gatctcgaac agggtgtcca cggcgcctgc
     1501 tttgaggtca cgcgcaccgt aaacgagccg cgcgacgcgc gagacgacga gcgcaccggc
     1561 gcacatgggg caaggctcga gcgacgagta aacggtcgcg ccctcgaggc gccaatgacc
     1621 gatcaccgtc gccgcctttc gcagcgcgag cacctcggcg tgcgcggtgg gatcaccgag
     1681 ctcttcgcgg cggttgtggt cgcgggcgag cacctggccg tcggcaccga cgacgacgca
     1741 accaatcggc acgtccccgt gcagcacggc gctgtccgcc tcttccagcg cgaggcgcat
     1801 gaactcgacg tcggtcgatt caggcgtggc ctggtcgccg cgcaggaacc cgtccgtcgg
     1861 attcgtcatg cgcctcccgg gaactccgaa atcagccgaa atgcaggtag ggcagctttt
     1921 gcgcgagcgc cttcaccgct tgggtgccgt cgagcagctg acctatcgga tcccacacgc
     1981 cgttcatcag cgcgtcgcgc gcgctcggct tcatcgtgaa cggcaggctg agcggccccg
     2041 cgttcacctt gttcgcctga aggtcgatgc ggacctcggt gctcggctcc ttctcgacct
     2101 gcgccgcgag cttgacgatg tcgtcgtgcg aaagcgagac gcacggcatc ccaagcgtcg
     2161 tcgagttgcc gaagaagatc tcggcaaagc tctccgcgat gacggcgcga atgccgaact
     2221 tcgcgagcgc ttgcggcgcg tgctcgcgcg acgagccgca gccgaagttg ctgttcgaga
     2281 tcaggatctt cgcgccctcg aagcgcgggt cgttcagcgg gtgcgccttt ttcgagccgt
     2341 cttcgttgaa gcgcacgtcg tggaagaggt actgaccgag cccgtcgaag gtcacgcact
     2401 tcatgaagcg cgccgggatg atgcggtcgg tgtcgatgtc gttgccggcg acgtacacgc
     2461 cgcggccgct aacctggagg atcttttcga gtgccatcta gcgttctccc gtcgattcag
     2521 ctggcgccga acacttcgcg cgcgtcggcg attttgccgc tgatggcggc cgcggcgacc
     2581 atcaccgggc tcatcagcac ggtgcgaccg gtcgggctac cttggcgacc cttgaagttg
     2641 cggttcgagg acgaagcgca gagctcgcgt ccgttgagct tgtcggggtt cattgcgagg
     2701 cacatcgagc agcccggttg gcgccactcg aagccggcgt cgcggaa
//
LOCUS       DASTKS010000652         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1443615, whole genome shotgun sequence.
ACCESSION   DASTKS010000652 DASTKS010000000
VERSION     DASTKS010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443615"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFQ35_13500"
     CDS             <1..419
                     /locus_tag="VFQ35_13500"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9931708.1"
                     /translation="AMNYCGPAAQCNGTDPYQYLKDFFAACSSCEVDYVAVHWYNCDL
                     PSLKDYLEPGGNLPGFEQFGKPIWLTEFSCNGDASVAEQEAYMRAAIPYLEQSTKVFR
                     YSWFSADPIPNARLLDANGGPTALGRVYIDLPQSCR"
     gene            594..1691
                     /locus_tag="VFQ35_13505"
     CDS             594..1691
                     /locus_tag="VFQ35_13505"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET9931709.1"
                     /translation="MCALLAACGAAPNDADESVASDNAELSRGRTSVVYVESNDANAN
                     SVLAFVRRDDGQLIKLAEAPTGGLGLGRSLGSQGAVATALDGRFVLAVDAGSNEISAF
                     RARGAHLELRSRVPSGGVRPVSVAAHGPFVYVVNQGEPAMVTGFRLNDEGTLVPIAEA
                     IQPLSAPNANPAQVGVSPSGDAVVVSEKASNVLSVFRVTRRGGLEPAESVPSAGTTPF
                     GFAFDGRGDLVVSEAATMSASSYRFDRHGELVNVSAVVSDHQTAPCWVATSADSRYAY
                     VANAGSASISGYRIDVHGSLSLLDETGVTAATGEGSRPLDMAVDGDRHLYLLDAGNHA
                     LVEYAIERDGALRYEAKQANLPSFAAGVAAR"
     gene            complement(1705..2040)
                     /locus_tag="VFQ35_13510"
     CDS             complement(1705..2040)
                     /locus_tag="VFQ35_13510"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HET9931710.1"
                     /translation="MRAELVAIAEALLRRDEEELSLDVIAEALGTLNVDSEEIDGLFA
                     FLEERGRRIGEGAIGKASASLADVLRTARALRASLGRTPSVREIAEQSGVPVDAVRRA
                     LLFSQIVQR"
     gene            complement(2040..>2308)
                     /locus_tag="VFQ35_13515"
     CDS             complement(2040..>2308)
                     /locus_tag="VFQ35_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9931711.1"
                     /translation="ASLSHVMRRNLPACSADASLVQVRERIAETGLPVVVLSENRLMG
                     ILSAEDLARITELSSRLASLGIRRPPTGPAEELAPPTSADAKGT"
BASE COUNT          385 a          743 c          783 g          397 t
ORIGIN      
        1 cggcgatgaa ctactgcggc cccgccgccc aatgtaacgg gacggatccg tatcagtact
       61 tgaaagactt cttcgcggcg tgctcgagct gcgaggtcga ttacgtcgcg gtgcattggt
      121 acaattgcga tttgccgtcg ctcaaagact atctggagcc gggcggcaac ctgcccggct
      181 tcgagcaatt cggtaagcca atttggctca ccgagttttc ttgcaatggc gacgcgagcg
      241 tggccgagca agaggcttac atgcgcgcgg ccatcccgta cctcgagcag agcacgaagg
      301 ttttcaggta ctcgtggttc agcgccgacc caattcccaa tgctcggctc ctcgacgcga
      361 acggcgggcc caccgcgctc gggcgcgtgt acatcgactt gccgcagagc tgccgctgac
      421 gcagcggtcg gcgcgtgtcc gggcgaatcc ggtcgggcgc gcgtatctgg gttccatcgc
      481 gtatcgaggt aaccggccgc tgcgagctcc gtagggaggt gcgaagcggg acgaacccgc
      541 cgagagccgg aggatgcctt tcgatgcgat gcaatttgct aggtttgacg agtgtgtgcg
      601 ctctacttgc cgcttgtggg gccgctccca acgacgctga cgagagcgtc gcgagcgaca
      661 acgcggagct cagccgaggg cgaacttctg tcgtctacgt cgagagcaac gacgcgaacg
      721 cgaacagcgt gctcgcgttc gtacgccgtg acgacggtca gttgatcaag ctcgccgaag
      781 cgccgacggg aggcttggga ttgggccgaa gcctcggctc acaaggcgcc gtcgcgaccg
      841 cgctggatgg tcgcttcgtg ttggcggtcg acgcggggag caacgaaatc tccgcgtttc
      901 gtgcgcgcgg cgcgcacctc gagctgcggt cgcgagtgcc ctccggtggc gtgcgtcccg
      961 tgagcgtggc ggcgcacggc cccttcgtct acgtcgtcaa tcagggtgaa cctgcgatgg
     1021 tcaccggctt tcgattgaac gacgaaggca ccctcgtgcc catcgccgag gcaatccagc
     1081 cgctcagcgc gcccaacgca aacccagccc aggtcggcgt gagcccgtcc ggcgatgcgg
     1141 tggtggtgag cgagaaggcg tcgaacgtgc tcagcgtctt ccgtgtcacg cgacgcggtg
     1201 gcctcgagcc cgccgagtca gtgccttcgg cagggaccac gccgttcggg ttcgcgttcg
     1261 acgggcgtgg cgacttggtc gtctccgagg ccgccacgat gtcggcgtcg tcgtaccgct
     1321 tcgaccgcca tggtgagctc gtgaacgtga gcgcggtcgt atccgaccat caaaccgcgc
     1381 cgtgttgggt cgcgaccagc gccgattctc ggtacgctta cgtggcgaac gccggtagcg
     1441 cttcgatttc gggctaccgc atcgacgtcc atggttcgct cagcttgctc gacgaaactg
     1501 gcgtgaccgc tgcaaccggc gaaggtagcc gcccgctcga catggcggtc gacggcgacc
     1561 ggcacttgta cttgctcgat gccggcaacc acgcgctcgt cgagtacgca atcgagcgcg
     1621 acggtgccct ccgctacgaa gccaagcagg cgaacctacc gagcttcgcg gcgggcgtgg
     1681 ccgctcgctg aaagttgcac gagctcagcg ctgaacgatc tgcgagaaca ggagcgcgcg
     1741 gcgcaccgcg tcgaccggta cgccgctctg ctcggcaatt tcgcggaccg acggtgttcg
     1801 accgagcgag gcgcgcagcg cgcgagccgt cctcagcacg tcggcgagcg aggcggatgc
     1861 cttgccgatc gccccctcgc cgatgcgacg accgcgctcc tccaagaagg cgaagagccc
     1921 gtcgatctct tcgctgtcga cgttgagcgt cccgagcgct tcggcgatga cgtcgagcga
     1981 cagctcttct tcgtcgcggc gaagcaacgc ctctgcaatt gccacgagct ccgcgcgcat
     2041 cacgttccct tcgcgtcggc ggaggtgggc ggagcgagct cctcggcggg gccagtcggc
     2101 ggacgacgga tcccgaggct cgccaggcgg gacgaaagct cggtgatgcg agcgagatct
     2161 tctgcggaca ggatgcccat gaggcgattt tcggagagca cgacgacagg cagacccgtt
     2221 tcggcgattc gctctcgaac ttgcacgagc gaagcgtccg cggagcaggc tggcaagtta
     2281 cggcgcatca cgtggctgag gctcgcgc
//
LOCUS       DASTKS010000653         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_772325, whole genome shotgun sequence.
ACCESSION   DASTKS010000653 DASTKS010000000
VERSION     DASTKS010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772325"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFQ35_13520"
     CDS             <1..578
                     /locus_tag="VFQ35_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9931712.1"
                     /translation="DIFSGGGRGRANARGSDLQSEVVVDFVSALRGAELKLRLQDGGD
                     EVTVRVPAGAGDGDKVRVPGQGAPGVGGGPPGDLLLTIRVRPHQFFERSGLDLYLDLP
                     ITVGEAFRGAKVRVPTPDGPVTLSVPKHAQSGQVARLKGKGVKRGDNTGDLFVRFQIK
                     LPTAASPEVEKAVQALEDAMTGDVRAGIVFA"
     gene            complement(583..1761)
                     /gene="add"
                     /locus_tag="VFQ35_13525"
     CDS             complement(583..1761)
                     /gene="add"
                     /locus_tag="VFQ35_13525"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9931713.1"
                     /translation="MDSHDARPSSTADRPRPPVVPFEVIRALPKTDLHVHLDGSLRLE
                     TILDIAKQEGIELPASDVAGLREAIGCGRHFGSLVEYLRGFDITLRVMQTEESLERIA
                     FELAEDAHQENVRYMEVRYAPMLHTRRGLKLTRVVEAVLLGLRRARETYGIKANVIVC
                     GIRNISPESSYEMAELAVAYKGRGVVGFDLAGAEADFPAKHHRAAFQLVRDNNINCTI
                     HAGEAYGPESIAQAIHVCGAHRIGHGCRLRENGDLLHYVNDHRIPLECCPSSNVQTGA
                     VRDLRSHPLKLYFDLGLRVTVNTDNRLITDTTVSKELYLVHTELGVPFNDIKSMIVSG
                     FKSSFQPFHEKQALLRKVAQELARYDEHGNVREMPPGPERTSRARATAELAAIQSGGE
                     "
     gene            complement(1824..>2640)
                     /locus_tag="VFQ35_13530"
     CDS             complement(1824..>2640)
                     /locus_tag="VFQ35_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9931714.1"
                     /translation="HFCWLCLVTDVSILTGGIVSVSYLRTPPTERRRAPLKNWAWSVL
                     ALVAVVAPTLWPQLRPLPPVPSAIHAYYRSGKINVIEFSDFECPFCRDLHGRLHALLE
                     PYGDKVHFVRLNMPLASHEFARDAALASICAEPSGKAAQFAEFLYTTEDLSVPAIRRE
                     AERMGIDLTAFDACRSAPATLARLEREARALRDSGFQGLPTTYVGGRRIVGAQSDDTF
                     RAALEHAAAGDDVRGIPAWAYLLALTMLIVFVVRSGSLADPKPSDTASSPNKP"
BASE COUNT          462 a          813 c          922 g          443 t
ORIGIN      
        1 gggacatctt cagcggcggt ggccgtggac gagcgaacgc gcggggctcc gacctacaga
       61 gcgaggtcgt cgtggacttc gtgtccgcgc tccgcggtgc ggaactgaag ctgcggctgc
      121 aagacggcgg cgacgaggtc acggtgcgcg tcccggctgg cgctggggac ggcgacaaag
      181 tgcgcgttcc tggacaggga gcccccggcg tcggcggagg tccccccggc gacctactgc
      241 tgacgatccg tgtgcgcccg catcaattct tcgagcgctc ggggctcgac ttgtacctcg
      301 acctgccgat cacggtcgga gaggccttcc gcggcgccaa agtgcgcgtc cccacgccgg
      361 acgggccggt gacgttgagc gtaccgaagc acgcgcagag cgggcaggtc gctcgcctca
      421 agggtaaagg cgtgaagcgc ggcgacaata cgggagacct cttcgtgcgc ttccagatca
      481 agctgccgac cgccgcttcg cccgaggtcg agaaggccgt tcaggcgttg gaagacgcga
      541 tgaccggcga cgtgcgcgcc ggcatcgtct tcgcctgagc tcctactcgc cgccgctttg
      601 gatggcggcg agctccgcgg tggcccgggc gcgggacgtg cgctcgggac cgggcggcat
      661 ctctcgaacg ttgccgtgct cgtcgtaacg cgcgagctct tgagcgactt tgcggagcag
      721 cgcctgcttc tcgtgaaacg gctggaagct cgatttgaag cccgaaacga tcatcgattt
      781 gatgtcgttg aaggggacgc cgagctcggt gtgcacgagg tagagctcct tcgagaccgt
      841 cgtgtcggtg atgagacgat tgtcggtgtt cacggtcacg cgcaatccga gatcgaagta
      901 gagcttgagc gggtgcgagc ggaggtcgcg cacggcaccg gtttgcacgt tgctcgacgg
      961 acagcactcg agaggaatgc ggtggtcgtt gacgtaatgt aggaggtcgc cattctcgcg
     1021 gagacggcag ccgtggccga tgcgatgcgc cccgcagacg tggattgcct gcgcgatcga
     1081 ctccgggccg tacgcttcgc cggcgtggat ggtgcaattg atgttgttgt cacgaaccag
     1141 ctgaaaagcc gcgcgatggt gcttcgccgg aaaatccgcc tcggcgccgg ccaagtcgaa
     1201 gccgacgacg ccgcggccct tgtaagcgac cgcgagctcc gccatttcgt acgacgactc
     1261 cggcgagatg ttgcgaatcc cgcacacgat gacgttggct ttgatgccgt aagtctcgcg
     1321 agcacggcgc aagccgagca gcactgcctc gaccacgcgc gtgagcttca gtccgcggcg
     1381 tgtgtgcagc atcggcgcgt agcgaacctc catgtagcgg acgttctcct ggtgcgcgtc
     1441 ctcggcgagc tcgaaggcga tgcgctcgag agactcttcg gtttgcatca cccgcagagt
     1501 gatgtcgaag ccgcgcaggt attcgacgag cgacccgaag tgacggccgc agccgatggc
     1561 ctcccgcagc ccggcgacgt cgctcgcggg gagctcgatg ccctcttgtt tggcgatatc
     1621 gaggatggtt tcgagccgta gcgaaccatc caggtggacg tgtagatccg tcttcggcag
     1681 cgcgcgaatc acctcgaatg ggacaaccgg gggacgcggt cggtcggcag tcgagctcgg
     1741 gcgggcgtcg tgactatcca tgggtgagtc taacacgagc actcgagttg tcaggtgagc
     1801 cgactcgcgg actcgaggtc gcgtcagggc ttgttcggtg acgacgcggt gtcgctcggc
     1861 ttcgggtcgg ccaggctgcc gctgcgtacg acgaagacga tcagcatcgt cagcgcgaga
     1921 agatacgccc acgcgggaat gccccgaacg tcgtcgcccg cggccgcgtg ttcgagcgcg
     1981 gcgcggaacg tatcgtcgct ctgcgcgccg acgatgcgac gtccacccac atacgtggtg
     2041 ggcaagccct gaaatccgga gtctcgcagc gcgcgtgctt cgcgctcgag gcgagcaagg
     2101 gtcgcgggag ccgaacgaca agcatcgaaa gcggtgaggt cgatccccat ccgctccgcc
     2161 tcgcgccgaa tggcgggcac ggacaggtct tctgtcgtgt agaggaactc ggcaaactgg
     2221 gccgctttgc cgctcggctc ggcgcagatc gaagcgagtg ccgcgtctcg cgcgaactca
     2281 tgactcgcga gcggcatgtt gagccgtacg aagtgaacct tgtcaccgta cggctcgagc
     2341 aaggcgtgca accgaccgtg caaatcccga cagaacgggc actcgaagtc ggagaactcg
     2401 atgacgttga tcttgccgct gcggtagtac gcgtggattg cggatgggac cggaggcagc
     2461 ggccgaagct gcggccacag cgtgggcgca accaccgcga cgagcgcgag gacgctccat
     2521 gcccagttct tcagcggcgc tcttcgacgc tctgtcggcg gcgtgcgcag atacgaaacc
     2581 gacacgattc cgccagtcag aatcgacacg tcggtcacca ggcagagcca gcagaagtgg
//
LOCUS       DASTKS010000654         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1891088, whole genome shotgun sequence.
ACCESSION   DASTKS010000654 DASTKS010000000
VERSION     DASTKS010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891088"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFQ35_13535"
     CDS             <1..276
                     /locus_tag="VFQ35_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931715.1"
                     /translation="VNEHCVLPVREVSSLCRWADEFLRLGVLLDPATVLASATRGANT
                     AVVAWRAEDLCYAALCATRVASAVLTDRVANRRARSPNREQARGAPP"
     gene            273..1355
                     /locus_tag="VFQ35_13540"
     CDS             273..1355
                     /locus_tag="VFQ35_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931716.1"
                     /translation="MTKILIVEDDAAQARQLAKAISVRRPNYTVFTAPNGAAATALLR
                     DREADVVLTDLQMPEMNGFELIAWLLEHRPTVAVFIMTAYGNALTEQQLSAYGPVACF
                     TKPVDVEKVLERIGDSLSHDVRGHVKNMSLPPFLQLLELERMSCTLNVEHEGRRGTLF
                     VQKGELLDARVGELSGEPAALEVIAWNCAAITISANAPGVTRAIEKPIGFLIMDALRI
                     QDESARAKYIARSSLPPRKLEAAAFPLPTNALGVSLIDTRTGLVLAHGERPGVPLSEL
                     SDMALTILTQEERVLSSGEGDELIEEVVITGHDSCELIRRVPGHESLIVLMVFKTEET
                     NLVIARLELERLLRDTAWHALSPAAV"
     gene            complement(1467..2111)
                     /gene="maiA"
                     /locus_tag="VFQ35_13545"
     CDS             complement(1467..2111)
                     /gene="maiA"
                     /locus_tag="VFQ35_13545"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET9931717.1"
                     /translation="MISLYSYFRSSCAWRVRIALALKGLSFELFPVHLVRDGGEQHRP
                     EFHKQNPLEQVPVLEVNLGSTLFRLTQSMAILEYLEELYPNPALLPEGAEQRARVRQL
                     SEIVNSGIQPLQNLRLQQEVRAHGIDPAPLVQGFIQRGLEALEHIARGSASRFLVGNQ
                     VTFADVFLVPQLGAARRMNVNVEAYPLLRRIERECEALEPFQVAHPERQPDYEP"
     gene            complement(2108..3085)
                     /locus_tag="VFQ35_13550"
     CDS             complement(2108..3085)
                     /locus_tag="VFQ35_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9931718.1"
                     /translation="MRFATLRDGTRDGALIVVDPTGRRFARAGDIAANLQSALDRWPE
                     VEPKLRARADRLAHDPTAGEPLRVDELLAPLPRAYEWVDGSAYINHIVLVRKARHADP
                     PASLETDPMVYQGGSGVLLAPTQPLELPDARWGLDFESEIALVLGDVPLGTSVSEAAG
                     TVRLLMLANDVTYRELVPSELAKGFGFFQSKPATAFSPFAITPDELGERFREGRAFLR
                     LVSTLNGSIVGDTETGPEMHFSFFDLIAHIARTRAFTAGTVLGSGTVSNRDPARGVSC
                     LVERRTREIIEGGVARTPYLEVGDVIRIEAFTEDGKSPFGAIEQRVVEP"
BASE COUNT          595 a         1045 c         1116 g          581 t
ORIGIN      
        1 gtgaacgagc actgcgtctt gccggttcgc gaggtttcgt ccttgtgccg ctgggctgac
       61 gagttcctgc gtttgggggt gctgctcgac ccggcgaccg tactcgcttc tgcgacgcgc
      121 ggcgcgaaca cggccgtcgt cgcctggcgc gcggaggacc tgtgctacgc ggcgctgtgc
      181 gcaacgcgcg tggcttcagc ggtcctgacg gatcgggtcg cgaatcggcg cgcacgctcc
      241 ccgaaccgcg agcaagcgag aggagcaccg ccttgacgaa gattctcatc gtcgaggacg
      301 acgccgccca ggctcgccag ttagccaagg ccatcagcgt gcgccgtccg aactacaccg
      361 tcttcaccgc gccgaatggg gccgcggcga cggccctgct gcgcgatcga gaggccgacg
      421 tcgtcctgac cgacctgcag atgccggaga tgaacggctt cgagttgatc gcgtggttac
      481 tcgagcatcg gccaaccgtg gccgtgttca tcatgaccgc gtacggcaac gccctgaccg
      541 aacagcagct ctccgcgtac ggcccagttg cgtgcttcac gaagccggtc gacgtcgaaa
      601 aggtgctcga gcgcatcggc gacagccttt cgcacgacgt gcgcgggcac gtgaagaaca
      661 tgagtttgcc accatttttg caactcctcg agctcgaacg gatgagctgc acgttgaacg
      721 tggaacacga aggacggcgc ggcacgttgt tcgttcaaaa gggagaactg ctcgacgcgc
      781 gcgtgggtga gctctcgggc gaaccagcgg cgctcgaggt gatcgcctgg aactgcgccg
      841 cgatcacgat atctgccaac gcccccggtg tgacgcgagc gatcgaaaag cccatcggct
      901 ttctcatcat ggatgcgctg cggattcagg acgaaagcgc ccgtgccaag tacatcgctc
      961 gttcctcgtt accgccgcgc aagttggagg cggcggcctt cccattacca acgaacgctc
     1021 tcggcgtctc gttgatcgac acccgcacag gcctcgtgtt ggcacacgga gagcgccccg
     1081 gagttccact gagcgagctc tcggatatgg cgttgacgat actgacgcaa gaagagcgcg
     1141 ttctctcgtc tggtgaaggg gacgagctca tagaagaggt cgtcatcacc gggcacgact
     1201 cgtgcgagct catccgccgc gttccggggc acgaatcgct gattgtgttg atggtcttca
     1261 aaacagagga gacgaacctc gtcatcgcgc gactcgagct cgagcggctc ctgcgcgaca
     1321 ccgcgtggca cgcgctgtct ccagcggcag tctaggggct cgtcctgcgg tcgaagttgg
     1381 cacccgtcga tcggcctccg aactcgctcc ctgaaagtct cctcctacgg cgggaaccga
     1441 cacaaacggc acagcctgct agcgcgctac ggctcgtagt cgggctgtcg ctcggggtgc
     1501 gcaacctgaa agggctcgag cgcctcgcat tcccgctcga tgcggcgaag cagcggataa
     1561 gcttcgacgt tgacgttcat acgacgcgca gcgccgagct gagggacgag gaatacgtcg
     1621 gcaaaggtga cttggttgcc gaccaggaac cggctcgcgc tgccccgcgc gatgtgctcg
     1681 agcgcctcga gcccgcgctg aatgaagcct tgaaccagcg gcgctgggtc gatgccgtgc
     1741 gcgcgcacct cttgctgcag acgcaagttc tgaagcggtt ggatgccaga gttgacgatc
     1801 tccgagagct gccggacgcg ggcgcgttgt tcggcgccct cggggaggag tgccggattt
     1861 gggtagagct cctccaagta ttcgaggatg gccatcgact gcgtgagccg aaacagtgtg
     1921 ctgcccaggt tcacctcgag gaccggcact tgttcaagcg ggttctgctt gtgaaactcg
     1981 ggacggtgct gttccccccc gtctctcacg aggtgcaccg ggaagagctc gaagctgagg
     2041 cccttcagag cgagagcgat gcgtacgcgc caagcgcagc tgctgcgaaa atagctgtag
     2101 agacttatca tggttccacc actcgttgct cgatcgcgcc aaaagggctc ttgccgtctt
     2161 ccgtgaaggc ctcgatgcga atcacgtcgc cgacttctag gtacggcgtg cgcgcgacgc
     2221 cgccctcgat gatttcgcgt gtgcggcgct cgaccaaaca cgagacgccg cgcgccggat
     2281 cgcggttcga gacggtgccg ctgccgagca cggtgccggc cgtgaaggcg cgagtcctcg
     2341 cgatgtgcgc gatcagatcg aaaaatgaga aatgcatctc cgggccggtc tcggtgtcac
     2401 cgacgatgct cccgttcagc gtgctcacga gccgcaggaa ggcccgcccc tcgcggaagc
     2461 gctcgccgag ctcgtccggc gtgatggcga acggagagaa cgcggtcgcc ggcttcgact
     2521 ggaagaagcc gaagcccttc gcaagttcgc tgggtacgag ctcgcgatac gttacgtcgt
     2581 tcgccagcat gagcaggcgc accgtccccg cggcctccga caccgacgtc ccgagcggaa
     2641 cgtctccgag gaccagtgct atctccgact cgaagtcgag cccccaacgc gcgtctggca
     2701 actccagggg ttgagtgggc gcgagtaaga ctccggagcc gccctgataa accatgggat
     2761 ccgtctcgag cgaggcagga ggatccgcat ggcgcgcttt ccggacgagc acgatgtgat
     2821 tgatgtaggc ggagccgtcg acccactcgt aagcgcgagg tagcggcgcg agcagctcgt
     2881 cgacgcggag cggctcaccc gcggtcggat cgtgggcgag gcggtcggcg cgggcgcgca
     2941 atttgggctc gacttcgggc cagcggtcga gagcgctctg caggttcgct gcaatgtctc
     3001 cagcgcgcgc gaagcgtcta ccggtggggt cgactacgat gagtgcgcca tcccgggtgc
     3061 catcgcgcaa cgtggcgaac cgcatgaccg cgagcgtagc aagttttgcg ggcggactgt
     3121 cgagagactt ggcggcacgg ggcgctcgtt gggcgcggcg ctctgggtga ggtatgctgg
     3181 gggatgcagg ctgcgaaaga acgcgtgccg gcacacctcc ggcgcttcgt ggtccagcag
     3241 gactatgagc gatataccgc ggtcgatcag gcggtgtggc gtttcgtcct gctgcagacg
     3301 tactcgaaat tggtcgatac ggcccaccca gcgtacc
//
LOCUS       DASTKS010000655         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1891607, whole genome shotgun sequence.
ACCESSION   DASTKS010000655 DASTKS010000000
VERSION     DASTKS010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891607"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..791
                     /locus_tag="VFQ35_13555"
     CDS             309..791
                     /locus_tag="VFQ35_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9931719.1"
                     /translation="MQLTLFSDYSLRILLYLAAHRGERIALPEISAAYGISQHHLVKV
                     VQRLIEEGWVESTRGRGGGLTLACEPAAINVGTVVRATEPHLNLVECFDSVSNTCPID
                     GACGLKRALARARDAFFEELERHTLADFAPKAPALIKLWKQQLDVDGTRPTPAQARKT
                     "
     gene            complement(839..1525)
                     /locus_tag="VFQ35_13560"
     CDS             complement(839..1525)
                     /locus_tag="VFQ35_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931720.1"
                     /translation="MKADPTTPAVTPPAASAGGGAHAAASAAAASASGSAARSAAASA
                     ARLRRRMFFVGGVAAAAGIALGVLGVSWNDRRASDFLVNEVVNDHLRVLYDAHPTEVE
                     SGGLHQVKPWFEGRLDFAPVLDFEGDNDFTLTGGAVGYFVDRKAAVFLFKRRLHIVSV
                     LVFRADGLPWPHVGTKKLGRVNASVSRSRGFSTVLYRDGDLGYALVSDVDPPTLERLA
                     EKLAPAPQEK"
     gene            complement(1732..2220)
                     /locus_tag="VFQ35_13565"
     CDS             complement(1732..2220)
                     /locus_tag="VFQ35_13565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931721.1"
                     /translation="MLEQVDALYAFAYRLSRSGAEAEDLVQETFTRALAATQQFNPSS
                     NLRAWLFTILRNAFIDARRRARRAPLPDESAIEGASDPNLELLRGDLELDRMRRLVGE
                     DIEAALQQLSDDARTVVLLDLEGFTEAEIAGVMGCAAGTVKSRLARARAALRSKLAEY
                     GK"
     gene            2460..>3015
                     /locus_tag="VFQ35_13570"
     CDS             2460..>3015
                     /locus_tag="VFQ35_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9931722.1"
                     /translation="MKPNAPVRIGQQLLERRELLKLLSFGGVVSAAGLFGCASAASQK
                     LPANPAAPTPNEGPPARSLPAAPEDFVFLQLSDTHWGYRGDANPEAASTLRRAVDAIN
                     ASSLEPDFVVFTGDLTHTTDDSSVRRARMKEFRAIVDGLKVKTRYFIPGEHDASPDGG
                     EAYRDAFGETHASFDHKGVHFTLLD"
BASE COUNT          563 a          966 c          965 g          521 t
ORIGIN      
        1 ggtgagaaaa gccttttgct tcgcacgctg tcgatccatg tcgacggttt cgaagtaggc
       61 cgcgacgcgc tcgtcgagca gcatcttgcg gtagaagacg tcgacggcct tatcaatcgc
      121 tgcttctccg cccaactgct cgtaaatgct cgacatatat caacgctcca tgggtgtgag
      181 gagctcgagg cgcgaaacgt gctcgacgaa cgcagctccc gagcgcgcgg cggcacgaac
      241 tctcgagccg ccgtttaact tgtatccgaa atacaagtta tgctaccaac tcgtcaaccc
      301 caatcgtcat gcagctcaca ctcttttccg actactcgct gcgcatcttg ctgtacttgg
      361 cggcgcaccg gggcgagcgc atcgcgctac cagagatcag cgcagcgtac gggatctcac
      421 agcatcacct cgtgaaggtg gtgcagcgct tgatcgagga aggatgggta gagagcacac
      481 gtggacgcgg cggtgggctc acgctcgcct gtgaaccggc cgccatcaat gtgggcaccg
      541 tcgtgcgcgc caccgagcct cacttgaacc tcgtcgagtg cttcgattca gtttcgaaca
      601 cttgtccgat cgacggcgcc tgcgggctga aacgagctct cgcgcgggct cgcgacgctt
      661 ttttcgaaga gctcgagcgc cacacgctgg cggactttgc gcccaaggcg cctgcactca
      721 tcaagctgtg gaagcagcag ctcgacgtcg atgggacgcg cccgacgccg gcgcaggcac
      781 gcaaaaccta ggcgctggcc cgacgtagac gcgcagagcc taggtgcgct ggtcgcggct
      841 atttttcttg cggcgcaggc gcgagctttt cggcgaggcg ctcgagggtc gggggatcga
      901 cgtcggagac gagtgcatag ccgagatctc catctcgata cagcacggtg ctaaagccac
      961 gagagcggct taccgaagcg ttgacgcggc ccaacttttt cgtgccgacg tgcggccacg
     1021 ggagcccatc cgcgcggaag acgaggacgc tgacgatgtg cagacgacgt ttgaagagga
     1081 agacggccgc cttgcgatcg acgaagtagc cgaccgctcc gcccgtgagg gtaaagtcgt
     1141 tgtcgccctc gaaatcgagt accggcgcga agtcgaggcg cccttcgaac cagggcttta
     1201 cttgatgtag cccgccgctt tcgacctcgg tcgggtgcgc atcgtagagc acccgcaagt
     1261 ggtcgttgac cacctcattg acgaggaagt ccgaggcgcg gcggtcgttc caagacacac
     1321 ccagaacgcc gagggcgatc ccggccgcgg cggcgacccc tcccacgaaa aacatgcgac
     1381 ggcgcaagcg agccgcagaa gccgcggcgc tccgcgcagc agagccggaa gcggatgcag
     1441 cggcggccga ggcggcggcg tgggcaccac cgccggcgga agcggcaggg ggagtgacgg
     1501 cgggcgttgt gggatcggct ttcatttgga gtcgcgcttt cagcgaagtc ggcgccgcgc
     1561 ggggcggaag gcgcgcgagt gcttggtcta gagcctgctc gttcttttgg aaggtccggc
     1621 aatcttcgca agtttcgagg tgcgcggcga cttctcggtg ctgttcacgc gaaagaccga
     1681 ggcgcgtgaa gtcgcggagg aggtcgcgaa cctgttcgca gcgtaaagag gtcatttgcc
     1741 gtactccgcg agcttcgagc gcagcgcggc tcgggcgcgc gccaaccgcg acttgacggt
     1801 gcctgcggcg caacccatga cgcccgctat ctcggcttcg gtaaagcctt cgaggtcgag
     1861 cagcacgacg gtgcgagcgt cgtcgctcaa ctgttgtaac gcggcctcga tgtcttcacc
     1921 gactagtcgg cgcatccgat cgagctcgag gtcgccacgt agcagctcta ggttcggatc
     1981 gctggcgccc tcgatcgctg actcgtcggg cagcggcgcg cgtcgggcgc gacgacgagc
     2041 gtcgatgaat gcattgcgca aaatcgtaaa gagccaggcg cgcaggttcg agctggggtt
     2101 gaactgctgt gtcgcggcca aggcgcgcgt gaacgtttct tgcacgagat cttcagcctc
     2161 ggccccactc cggctcagcc gataagcaaa tgcgtagagc gcgtcgacct gctccagcac
     2221 tcggcgggaa aaagtcgatt gcgctgtggg gtccggcacc cgactcgaat accagctgga
     2281 cctaaccccc gacaagcgcg cgactcctcg cgagaggggc gccggagacg tattcgtagc
     2341 caatttgcct cagtaacgcg cgagaccgct cttggttccc cgaaaaacgg agattttcgg
     2401 ccgcaccagc cattctcgat ttgtcggaac caacccgcac tggcttcgtt gagctcggca
     2461 tgaagccgaa tgctcccgtc cgaattggcc aacaactgct cgagcgccgt gaactcctca
     2521 agctgctctc ctttggcggc gtcgtgagcg ccgctgggct cttcggctgc gcctcggctg
     2581 cgtcgcaaaa gctacccgcc aacccggccg cgcccacgcc aaacgaaggt ccgccggcgc
     2641 gctcgctccc tgccgcgccg gaggacttcg tgttccttca gctcagcgac acgcattggg
     2701 gctatcgcgg ggacgcaaac cccgaagctg cgtccacgct gcgacgcgcg gtcgacgcca
     2761 tcaacgcctc gagcctcgag cccgacttcg tcgtattcac aggcgacctc acccacacga
     2821 ccgacgattc ctcggtacgc cgcgcgcgca tgaaagagtt tcgcgcgatc gtcgatgggc
     2881 tcaaggtgaa gacgcgctac ttcattccgg gcgagcacga cgcgagcccc gacggcggcg
     2941 aggcctaccg cgatgcattc ggagagacgc acgcgagctt cgaccacaag ggcgttcact
     3001 tcacgctgct cgaca
//
LOCUS       DASTKS010000656         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_328423, whole genome shotgun sequence.
ACCESSION   DASTKS010000656 DASTKS010000000
VERSION     DASTKS010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328423"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFQ35_13575"
     CDS             complement(<1..1349)
                     /locus_tag="VFQ35_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9931723.1"
                     /translation="MIERFPYGRAPFWLLVLAVGSTCALLFIGRRGRERRADLTFALF
                     APNHLPAYNRVTPAFERKHGVRIAFQRVEPRALQTRLQNAMLSGTEVPDLVELPEGAL
                     TFFTRGPLKDVGFLDLTQRLEREGYRQRLVESRLSLWSSRRHVFALPHDVHPVMLAYR
                     ADLVESLGIDVSKLTTWDEFAAVGRRVVKDLDGDGVPDRYMLDLPLAATWGVTVLLRQ
                     RGVGLFDEQGRVAFNDPKTVETMIWYLHQTVGKERIAFECGEGQPLVKAMLDGLVLFY
                     LAPDWRTFVIEQQAPRLAGKMKLMPLPAWEPGGRRTSVWGGTGLAIAARSPHVELAWE
                     FAKFLYFDKSELGQRFLGTNIIPPLRDAWDLPEFKRRKAFYGGQALGEVFASLAPETP
                     PSWNTAYSKTAEAKLNAVFLRAVDHYRAHGDAGLQYVLTSLFFASVGTGVQLLFCSPR
                     "
     gene            1642..>2168
                     /locus_tag="VFQ35_13580"
     CDS             1642..>2168
                     /locus_tag="VFQ35_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9931724.1"
                     /translation="MNGRIYYAAPARPVLHAFSPGRTLGTMASVNLSDVVKVYDNGVR
                     VVDRMNLDIKDREFVVLVGPSGCGKSTTLRMIAGLEDVTSGSISIGGRVVNDVPPKDR
                     DIAMVFQSYALYPHMTVYENMAFALKLRKYSKSAIDEKVRHAAKILVIEHLLDRKPKA
                     LSGGQRQRVALGRAIV"
BASE COUNT          423 a          702 c          692 g          351 t
ORIGIN      
        1 cgcggcgagc agaacaagag ctgcacgccc gtgcccacgc tcgcgaagaa cagcgaagtg
       61 agcacgtatt gcaaacccgc gtcgccatga gctcgatagt ggtcgaccgc acgcaggaag
      121 acggcattga gcttcgcctc ggccgtcttc gagtaggcgg tgttccacga agggggcgtt
      181 tcgggcgcga gggaggcgaa cacctctccc agcgcttgcc ctccataaaa agcctttcgt
      241 cgcttgaact cgggcaagtc ccaggcgtcg cgcagcgggg ggatgatgtt cgttcccaga
      301 aagcgctgac cgagctcgct cttgtcgaaa tagagaaact tcgcgaactc ccacgcgagc
      361 tccacgtgcg ggctccgcgc tgcgatcgca agtcccgtac cgccccacac gctcgtccgc
      421 cgcccgcctg gctcccacgc ggggagcggc atcagcttca tcttgccggc caggcgaggc
      481 gcctgctgtt cgatgacgaa ggtgcgccaa tcgggcgcaa gatagaagag aacgagaccg
      541 tcgagcatgg ctttgacgag cggctgcccc tcgccacatt cgaaggcgat gcgctccttc
      601 cctaccgttt ggtgcaaata ccagatcatc gtctcgaccg tcttcgggtc gttgaaggcg
      661 acgcgaccct gctcgtcgaa caagccgacg cctcgctggc gtaagagcac ggtgaccccc
      721 caggtcgcgg cgagcggcaa atctagcatg tagcgatccg gcactccgtc gccgtctagg
      781 tctttcacga cgcgacgccc aaccgcggcg aactcgtccc acgtcgtcaa cttgctcacg
      841 tcgatgccga gcgattcgac gaggtcggct cggtaagcga gcatcaccgg gtgaacgtcg
      901 tgtggcaggg cgaacacgtg tcttcgtgag gaccacaggc tgaggcgcga ctcgacgagg
      961 cgctgacgat acccttcgcg ctccaggcgc tgggtgagat ccaagaaccc gacgtccttc
     1021 agcggcccgc gcgtgaagaa ggtgagcgct ccctcgggca gctcgacgag atccggaacc
     1081 tcggtgccgg agagcatcgc attctggagg cgggtctgca gcgcgcgcgg ctccacacgc
     1141 tggaatgcga tccgcacccc gtgcttgcgc tcgaacgccg gagtaacccg attgtacgcc
     1201 ggtagatgat tcggagcgaa cagggcgaag gtgagatccg cgcgccgctc ccggccgcgc
     1261 cgaccgatga agagcagcgc acaggtcgaa cccacggcga gcaccaagag ccaaaacggc
     1321 gcccttccat atgggaagcg ttcgatcatg gttggccgct ctagcccgcg gttcgcacgc
     1381 taaatcagcc cgcgcctttc ggcaacgacg tcccgacccg aactcggcag caccgctcga
     1441 gtagctcgga cccgcgggcc cttagggtcc gcgaatgaac aagtcagtcg cgagcgggtt
     1501 gagaccgcag cgaggcgggc gcgtgaggag agagcaccgc aggcctgctg aagcaggtcg
     1561 aggagcgcaa cgacgagccc gcccgccgca gcaagaggat cggcccgcgc agtatgactt
     1621 gtccattcga gggcccttag catgaacggt cgaatctact atgctgcgcc tgcgcgcccg
     1681 gttctccacg cattttcccc tggccgcact ctcggcacga tggcgtcggt caatctctcg
     1741 gatgtagtca aggtttacga caatggggtt cgcgtggtcg accgcatgaa tctcgacatc
     1801 aaagatcgag agttcgtggt gctcgtcggt ccctcgggct gcggcaagtc gacgacgctg
     1861 cggatgatcg cgggcctcga ggacgtgacg agtggcagca tctcgatcgg cggacgcgtc
     1921 gtcaacgacg tgcccccgaa ggatcgggac atcgcgatgg tgttccagag ctacgccctg
     1981 tacccgcaca tgacggtgta cgaaaacatg gcgttcgccc tcaagctccg aaagtactcg
     2041 aagagcgcga tcgacgagaa agtccggcac gcggccaaaa ttctggtcat cgagcacttg
     2101 ctcgaccgca agccgaaggc gctttcgggc ggtcagcggc agcgcgtcgc gctcggacgc
     2161 gccatcgt
//
LOCUS       DASTKS010000657         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_773927, whole genome shotgun sequence.
ACCESSION   DASTKS010000657 DASTKS010000000
VERSION     DASTKS010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773927"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1479
                     /gene="tssK"
                     /locus_tag="VFQ35_13585"
     CDS             187..1479
                     /gene="tssK"
                     /locus_tag="VFQ35_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HET9931725.1"
                     /translation="MNQPAQGMTRVHWHLGQALLPAHFMAQEESLRAEASLRFSMLPL
                     PAWGLGRLEFDTFLFGTTGVLSVRELSLVFESGTVLDIPGNAAPVSLDLKELGRSRTP
                     VYVHFKSDFTVVKLDPGHPGEEGIERLQQRAELSGDQAAGEQWFQLAEVRRGAQDAWE
                     FCPEYVPPLLRVRPGLLFDTQLERMRDIVNRLRHDLREELLANHLSGETHVLAKQALR
                     SLYGFQARLADLGGQVPPHPYELFSSLRGLYIDTCVLRNVQELEALEQPYDHRDIGAC
                     FQRILEPLSRLLSRGRPEVPYVEFSRKEGLLYCDVDKNVKRARDVFLLVQKPSVAAVV
                     DLSPEVSAEARQAGTTARVKLASPSRLHFVHERALTGIPYRRIERPPFAQSLSSTVDV
                     YALGRGQEWDYAVAEGRIVLFDIPALEQCRLYLYSRVD"
     gene            1504..>3713
                     /locus_tag="VFQ35_13590"
     CDS             1504..>3713
                     /locus_tag="VFQ35_13590"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HET9931726.1"
                     /translation="MSIVSELLVAVEKLPAVDAKGGLVTKLAGTSPLALLLGTLAAAA
                     LAYGGKRLVKFWGDRAAAKKALGPETLRPSTQPATPDIPPRQLRRAWLRFLLRLPREY
                     RRSILNFEHFVLLGRAGSGKSRLLETHTDYRYQMRQVAGDAPLDPELPVVLASGAIIT
                     ELPSRFLDDETLQGKVAIERLWGPLYTRRSPTVVLAVDATWLIDAAREDILELARCAR
                     SKVNILSAIRKKPVELRVAFTHLDGLDGARETARFWSQVGISACMPLDSAPTVEESVD
                     AWEREANAQLPRALGAVTAEQYRRVLAFTRGLPKLLAPLTTLVRSLFAVDLMSQTPLR
                     GGVFFCTDAPSSANPLRNAHDAGPGPNPLRQHVQLAVASVIVCVSYMTLAFNVQEDVW
                     APAADAAAAYEVSPALAGTERERTQRERIDEFTVKHAGILYRFPDFHGRVRRAMRAQL
                     STKLREALLVPRLQEVAQLGIARSNGMTLRWRRSVYFLSLIHSFVADQAGILPRLALW
                     QSMTELPPDLIRDYIANTDTPYQTPVLFKLAADAKDERDKPAFWEALPLEVNKALSDG
                     ILREEELTHLQSVIKPMTVAIERFQHDDVTLKVFANIDAAADIGGEHDGNPPPRLYDA
                     YLPQYDEILKGIGESDMQRQSGQISRLLTMVQNASVSGPEEKLLHDLNDRLAVLMHTS
                     KAIDVQPVEVELKHTKYFVDVKKWNEALSDGRAAVLINRFLAATARLDSIFFSQ"
BASE COUNT          632 a         1207 c         1216 g          658 t
ORIGIN      
        1 gcacttcgcg actgcttcgg ggaagaactt cgtgaagcgt tggggtctca aggttggaac
       61 ccaagccgcg gccgcgctcg cgcccgcaac ctgaggagag ggaaaccaaa gagctcggtc
      121 gatccgccgc tccctaccgc gggggcggcg gcgtcggcgg agcattctcg gacgcggtga
      181 gtggcgatga atcagcctgc acaaggaatg acgcgcgtgc actggcactt gggccaggcg
      241 ctgctgccgg ctcacttcat ggcgcaagag gagtcgctgc gcgcggaggc ttcgctgcgc
      301 ttttcgatgt tgcctctgcc cgcttggggg ctcgggcggc tcgaattcga cacatttcta
      361 ttcggtacca ccggcgtgct ctcggtgcgg gagctctcgc tcgtattcga atcgggaacg
      421 gtgctcgaca tccccgggaa cgcggcgccg gtgtcactcg acttgaagga gctcggccgc
      481 agccgcacgc cggtatacgt gcacttcaaa tcggacttca cggtggtcaa gctggatccc
      541 ggccatcccg gcgaagaggg catcgagcgg ctccagcagc gcgctgaact gtcgggcgac
      601 caagccgcgg gggagcaatg gttccagctt gccgaggtgc ggcgcggcgc tcaagacgcg
      661 tgggagtttt gccccgagta cgtcccaccg ttgctgcgtg tacgcccggg gctgctcttc
      721 gacacgcagc tcgaacggat gcgagacatc gtgaaccgcc tgcgtcacga tctgcgggag
      781 gagctcctcg cgaaccacct ctcgggcgag acgcacgttc tcgcaaagca agccctgcgc
      841 agcctatacg gcttccaggc gcggctcgcc gatttgggcg gacaggttcc accgcacccg
      901 tacgagctct tctcgagcct gcgcggcctc tacatcgata cgtgcgtact tcgtaacgtg
      961 caggagctcg aggcgctcga gcagccatac gaccaccgcg atataggcgc gtgcttccag
     1021 cgcatcttgg agccgttgtc gcgcttgctc agtcgcggtc gtccagaggt cccctacgtg
     1081 gagttttcgc gcaaggaggg gctcctttac tgcgatgtcg acaagaacgt gaagcgcgcg
     1141 cgcgacgtgt tcttgctggt tcaaaagccg agtgtggccg cggtggttga cctttcgcca
     1201 gaggtcagcg ccgaggcgcg tcaagcggga accaccgcgc gcgtgaagct cgcgagcccg
     1261 tcccggctgc acttcgtgca cgagcgtgcg ctgaccggca tcccctatcg tcgtatcgag
     1321 cgcccgccct tcgcgcagag cttgtcttcg accgtcgacg tttatgcgct cggtcgcggt
     1381 caagagtggg actacgcggt tgcagagggc aggatcgtgc tgttcgacat cccagccttg
     1441 gagcagtgtc ggctgtacct ctactcgcgg gtcgattgag cctttagcct tgagagtgag
     1501 aacgtgagta tcgtttccga gttgcttgtc gccgtagaga agctgccggc ggtggacgcg
     1561 aagggcgggc tcgtcacgaa gctcgcggga acttcccccc tcgcgctgct gctcgggacg
     1621 ctcgcggccg cggccctggc ctacggcggc aaacgcctgg tgaaattctg gggagaccgc
     1681 gcggctgcga aaaaggcgct cggaccggag actctgcgcc cgagcacgca gccggccacg
     1741 cccgacattc caccgcgcca gctgcgccgg gcgtggctcc gttttctact gcgactgccg
     1801 cgggaatatc gacgctcgat cctgaacttc gagcatttcg ttctgctcgg gcgcgccggc
     1861 agcggcaaga gccggctgct cgagacgcac accgactacc gctaccaaat gcggcaagtc
     1921 gcgggcgatg cgccgctcga tcccgagttg cccgtcgtgc tcgcgtcggg cgccatcatc
     1981 accgagctcc cctcgcgctt cctcgacgac gagaccctac aaggcaaggt cgccatagag
     2041 cgtctctggg gtccgctcta cacgcgccgg tcgccgacgg tcgtgctcgc ggtcgacgcg
     2101 acgtggctca tcgacgcggc gcgcgaggac atcctcgagc ttgcccgctg cgcgcgcagc
     2161 aaggtcaaca tcttgtcggc cattcgcaag aagccggtcg agctgcgggt cgcctttacg
     2221 cacctcgacg ggctcgacgg cgcgcgcgaa acggcgcgct tctggtctca agtggggatt
     2281 tcggcgtgca tgccactcga ctcggcgccc accgtcgaag agagcgtcga cgcttgggag
     2341 cgcgaagcca acgcgcagct tccgcgcgcg ctcggcgcgg tgaccgccga acagtaccgt
     2401 agggtcctcg cgttcacgcg cggcttgccg aagctcttgg ccccgctgac gacgctcgtg
     2461 cgctcgctgt ttgctgtgga tttgatgtcg cagactccgc tgcgaggcgg cgtcttcttc
     2521 tgcaccgacg cgccaagcag cgcgaacccg ctgcgcaacg cgcacgacgc cgggccgggg
     2581 cccaacccgt tgcgccagca cgtgcagctc gcggtcgcga gcgtcatcgt gtgcgtgagc
     2641 tacatgacgc tcgcattcaa cgtgcaggaa gacgtgtggg cgccggctgc cgacgcggcg
     2701 gcggcctatg aggtctctcc cgccctcgcc ggaacggagc gagagcgaac gcagcgtgag
     2761 cgtatcgacg agttcacggt gaagcacgcg gggatactct acaggttccc agacttccac
     2821 ggccgcgtgc ggcgagccat gcgcgctcaa ttgtccacca aactgcgcga agcgctgctg
     2881 gttccgcggc tgcaagaggt ggcgcagctc ggcattgctc gctccaacgg gatgacgctg
     2941 aggtggcgcc gctccgtgta cttcttgtcg ctgattcaca gcttcgtcgc ggatcaggcc
     3001 gggattttgc cgcgcctcgc gctctggcaa tccatgaccg agctgccccc cgacttgatc
     3061 cgcgactaca ttgcgaacac cgatacgccg taccaaacgc cggtcctctt caagcttgcc
     3121 gcggacgcga aagacgagcg cgacaagccg gcgttttggg aagctttgcc gctcgaagtg
     3181 aacaaggccc tcagcgacgg gattctgagg gaggaagagc tcacccatct tcaatcggtg
     3241 atcaagccga tgacggtcgc gatcgagcgt tttcagcacg acgacgtcac gctgaaggtt
     3301 ttcgccaaca tcgacgcggc ggcggacatt ggcggcgagc acgacggaaa cccgcccccg
     3361 cgtctctacg acgcatactt gccgcaatac gatgagatcc tgaagggcat cggcgaatcg
     3421 gacatgcagc ggcagtcggg gcagatctcg cggctgctca ccatggtgca gaacgcgagc
     3481 gtttcggggc cggaggagaa gctgctccac gacctgaacg accgcctggc ggtgttgatg
     3541 catacctcga aggcgattga cgtgcagccg gtcgaggtcg agttgaagca caccaagtac
     3601 ttcgtcgacg tgaagaagtg gaacgaagcg ctcagcgacg gcagggccgc cgtcttgatc
     3661 aaccgctttc tcgccgcgac tgcgcgactc gattccatct tcttttcgca aca
//
LOCUS       DASTKS010000658         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_106062, whole genome shotgun sequence.
ACCESSION   DASTKS010000658 DASTKS010000000
VERSION     DASTKS010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106062"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1894
                     /locus_tag="VFQ35_13595"
     CDS             332..1894
                     /locus_tag="VFQ35_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9931727.1"
                     /translation="MAHSTNSSLCSGTVVGERFTIRRPLGHGGMASVYEAHDTRLRQR
                     VAIKVLSSAFASDPGAVERFFREARAVVLLRNQHVVRVFDVGTLPDGAPYLAMEFLDG
                     QSLAALLSSSGPLPVSQVVDFTLQTLEALAEAHRAGIVHRDLKPDNLMLVRGEDALPQ
                     IKVIDFGISKLVGPEFTLKNLTLDSSFLGSPLYASPEQLNDAAHVDARTDLWSLGVVM
                     YELLAGEPPFNARSASALMVAICSDEPRRLEDLRADVPRELADVVARCLSKHVAFRFA
                     SARELAQALAPFAPGSQSRMSVARIARVHGNAWSQSSERASTLFSLPPAWTSATMIPP
                     PPREEPPRRGRAVSVVALALAVGAVGAIAFTSWIRARGAETDARANASQRREALVAPS
                     VEALGDAPSEGRAAPVTRLSNDAPGAPASGDALAVRTPELAPSNALAPPPFPVVGGAT
                     PSRRQPTKRSLAAPIASEGDVGSRAAMPEASAAPEMPAAPPAASAGAPSRAARRLDTS
                     NPFAPGSPRVSD"
     gene            2038..3549
                     /locus_tag="VFQ35_13600"
     CDS             2038..3549
                     /locus_tag="VFQ35_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9931728.1"
                     /translation="MSFRFRALRPAWSLALAAVIGSVHVPRTAHAEEPKSLEAAKAEA
                     RERFDRGLKLFNQLDNEGALAEFTRAYELVPHAVVLYNIALVYAAMSKPVQAKEAFDR
                     LLLAPAGLDPQKLERAMAERDQQAALIAEVEIASAVAGAQIEIDGIAVGKTPLSAPLK
                     LASGSHIIGVVGSGYVPLRKQVTLAGNTRTKLDFELVLADAVLAHIDVRTRIPKLEVL
                     LDGQFVGLTPLPASLAIVPGAHQIEARRVGYRTFSQGIEVGAGSTGAIDVNPDVDPTA
                     LSASGGQLELAISEPNAVVFIDGESRGQYQSPLILPQGEHIARVERADFFPIERRVTV
                     PVRGRVTVTIELEPTPEKRARYRSATVARRTWGYVATAAGALIAGGGGGFLIWNSQKK
                     SEKKDLFDAQAARHQPGGDCDPTMVQASTCDTELQLALDNLDSARKRDAYGIAGLIAG
                     GALLGTGLVLLIGNDDPNRYEPKPESDVFANLRVTPSAWFTPGGSGVGLVGSF"
BASE COUNT          638 a         1316 c         1290 g          725 t
ORIGIN      
        1 ttttgcagaa gggtcacatc gtcgagcagg gtcgtcacga agagctcctc gcgcacgggg
       61 gactctttgc gcggctgcac gacctccagt tcgcgcgcca ctccgacgct ccttcggccg
      121 aaacgcgagc aagctaaccc agcgcgagtg cggagcactt tcgctgcatt gcggcttcgc
      181 cgataggtta acgctgctgc tcagtcgacc agttgcgggg tgtcaaaggc gtaacaccga
      241 cttcggggcg agcgcggcga aaacctgact attgaccgac ggcacctctt cccgtagcgg
      301 ttcaaagagg gcgcgcgctt tcgagggatg gatggcgcat tcgacgaact cttcgttgtg
      361 ttccggcacc gtggtcggag aacgcttcac aatccgacgt ccactcgggc acggcggaat
      421 ggccagcgtc tatgaagcgc acgacactcg gctgcgccag cgcgtcgcga tcaaggtgct
      481 gtcctcggct tttgccagtg atcccggcgc ggtcgagcgc ttttttcgcg aggctcgcgc
      541 ggtggtgctg ctgcgcaatc agcacgtcgt gcgcgtgttc gacgtcggta ccttgcccga
      601 tggtgctccc tatttggcaa tggagttcct cgacgggcag agcctcgccg cgctcctttc
      661 gagctcgggc ccgctgccgg tgagccaagt ggtggacttt accctgcaga cgctggaggc
      721 gctcgccgaa gcacaccgcg cgggcatcgt tcatcgtgac ctcaagcccg ataacctgat
      781 gttggtgcga ggcgaagacg cgctgccgca aatcaaggtc atcgacttcg ggatatcgaa
      841 gctcgtaggt cccgagttca ctctcaagaa cttgacgttg gacagctcgt tcttgggctc
      901 gcccttgtat gcctcacccg agcagctgaa cgacgccgcc cacgtcgacg ctcgcaccga
      961 tttgtggtcg ctcggcgtgg tcatgtacga gctcctcgcc ggtgagccgc cgttcaatgc
     1021 ccgcagcgcc tcggccttga tggtcgcgat ctgctccgac gagccgcgcc ggttggaaga
     1081 cttgcgcgcc gacgtgccga gagaactagc ggacgtagtc gcgcgctgct tgagcaaaca
     1141 cgtggcgttt cgcttcgcga gcgctcgaga gttggctcag gccttggccc catttgctcc
     1201 tggctcgcaa agtcgcatgt cggttgcccg cattgctcgc gtgcacggta acgcgtggtc
     1261 tcagtcttcg gagcgggcat cgactttgtt ttcgctgccg cctgcttgga cgagcgcgac
     1321 catgattccg ccgccgcctc gcgaggaacc gccgcgacga ggccgcgccg tctccgtcgt
     1381 ggcgctcgct ctcgcggtcg gagcggtcgg agcgatcgcg ttcacttcgt ggattcgggc
     1441 gcgcggtgcc gagacggacg cgcgcgccaa tgcctcacag cgtcgcgagg cgctcgtcgc
     1501 accgtctgtc gaagcgctcg gcgatgcacc gagcgaaggg cgtgctgctc cagtaactcg
     1561 cctttccaac gatgcaccgg gcgcgccggc gtccggcgac gcgctcgcgg ttcgaacgcc
     1621 cgagctcgct ccttcgaatg cgctagcacc gccgcctttt ccggtcgtcg gcggcgcgac
     1681 gccttcgcga cggcaaccaa ccaaacgctc tctcgccgcg ccgatcgcga gtgaaggcga
     1741 cgttggctcc cgagccgcga tgcccgaagc ttccgcagcc cctgaaatgc ctgcggctcc
     1801 gccggctgcg tcggcgggcg cgccctcgcg tgcagcgcgc cgactcgata cgagtaaccc
     1861 gttcgcgccg ggctcacccc gcgtttcgga ttgactttag cggcggcaga gcggcgcggc
     1921 cccgtggccg aggtcgttcg ggcttgatcg tcgttttcgc gaggcgtgtt agcctcggct
     1981 ccggtttggt cgaccggcta gcgtcgtggg cgctccggtc tcgcctcgga gattcgtatg
     2041 agctttcggt tcagagcttt gcgcccggca tggtcgctcg cgctggctgc ggtcatcggc
     2101 agcgttcacg ttccccgcac cgcgcacgcc gaagagccga agtcgctcga agcggccaag
     2161 gcggaggcgc gcgagcgatt cgaccgcggg ctcaagctct tcaatcaact cgacaacgag
     2221 ggagcgctcg ccgagttcac tcgagcctac gagctcgtgc cgcacgcggt ggtcctgtac
     2281 aacatcgcgc tcgtttacgc ggcgatgtcg aagccggtcc aggccaaaga ggcgttcgat
     2341 cgcttgctgc tcgcgcccgc ggggctcgat ccacaaaagc tcgagcgcgc gatggcagag
     2401 cgtgaccaac aagctgccct catcgcagag gtcgagattg cgagcgcggt ggcgggcgcc
     2461 caaatcgaaa tcgacggtat cgcggtcggc aagacgccgc tcagcgcgcc gctcaagctg
     2521 gcgagcggca gtcacatcat cggcgtcgtt ggctccgggt acgtgccatt gcgcaagcag
     2581 gtgacgctcg ccggcaacac gcgcacgaag ctcgacttcg agctcgtact agcggacgcc
     2641 gtgctcgcgc acatcgacgt gcgtacgcgc atcccgaagc tcgaagtatt gctcgatggc
     2701 cagttcgtcg ggctcactcc gttgcccgct tcgctcgcga tcgttccggg cgcgcaccaa
     2761 atcgaggcgc ggcgcgttgg gtatcgaact ttttcgcaag gcatcgaggt cggtgcgggc
     2821 agcaccggtg ccatcgacgt aaaccccgac gtcgatccta ccgcgctctc tgcttccggc
     2881 gggcagctcg agctcgccat cagcgagccg aacgcggtcg tcttcatcga cggcgaatcg
     2941 cgagggcaat atcagagccc gctcatcttg ccccagggcg aacacatcgc gcgcgtcgaa
     3001 cgcgccgact tcttccccat cgagcgtaga gttaccgtac cggtgcgcgg gcgcgtcacc
     3061 gtcacgatcg agctcgagcc cacgccggaa aagcgcgctc gctaccgtag cgctaccgtg
     3121 gcacgacgga cttggggtta tgtcgcgacc gcggccggcg cgctcatcgc aggcggcgga
     3181 ggcggcttct tgatctggaa ctcacagaag aagtccgaga agaaggactt gttcgacgca
     3241 caagccgcgc gacatcagcc aggcggcgac tgcgacccca cgatggttca ggcgtcgact
     3301 tgcgataccg agcttcagct cgcgctcgac aatttggact cggcccgaaa gcgcgatgcc
     3361 tacggtatcg cgggcctcat cgccgggggc gccctcctcg gcaccggtct cgtgctcttg
     3421 atcggcaacg acgacccgaa ccgctacgag cccaagcccg agagcgacgt cttcgccaat
     3481 ttgcgcgtta cgccgagcgc ttggttcacc cccgggggta gtggcgtggg gctcgtcggg
     3541 agcttctgaa cggtcgcgcc agagcctctc ggcgcccccg tcgagcacta cccgcgctga
     3601 atcgcgcgtc ctgctcttcg agtcgtcggg cgcgagccga gcgcggagtc ggcttgcgcg
     3661 agcccaccga gcgcctggcc gcggcgttcg aacgggcgcg cggtgacgtc agggcactct
     3721 cgattcgcag ggccagcgca tccaaccgtt gactcgaggg ggacggtcag gcggcgtaag
     3781 tcggttcgag aacgctcccg ctgtaatcgc tcgcgatgcc tccgccgcgt gcgtcacgag
     3841 tgttctctcc gtttcggcag atttacgcgg taacgattcc gagaacgatt cgtgcgacga
     3901 ccgtgtgagt ttgtttctca aagcagtttt gggattgaaa caatcgggag ccaaggaagc
     3961 gcgaacgaa
//
LOCUS       DASTKS010000659         6280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_328776, whole genome shotgun sequence.
ACCESSION   DASTKS010000659 DASTKS010000000
VERSION     DASTKS010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328776"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..1551
                     /locus_tag="VFQ35_13605"
     CDS             535..1551
                     /locus_tag="VFQ35_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9931729.1"
                     /translation="MTIAYPSDRSGFFLLSICVSSLLVFTACIRKTNSGCTTDAECGA
                     NAKCAAGNCVMNSGLGGSDGQGGASSGGTSTASGGKTETVGSGGVGTGQGGKATGGGG
                     EMTASGGAMASGGSGGGAGPKGGASGSGGGNASGGAPPLVTEMIDDMEDGDGRILMMN
                     GRQGPWHTFNSGESTQMPAGPSITPEMAGANGSMLAMHTSGKGFAYAGIGFDLSNADT
                     APESAQSKAYDASAWTGVVFMAKGGPSASAKLRIEIPMRDFVPSDRGGTCSGSCWNVY
                     GFQMPMALGSSWQEVKVPFSSLVREDGSTKPAFDSKQIMSISFKQQKDDFDFWIDDVR
                     FYKE"
     gene            1605..2936
                     /locus_tag="VFQ35_13610"
     CDS             1605..2936
                     /locus_tag="VFQ35_13610"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET9931730.1"
                     /translation="MTVVSRAALGQDFKLGVATSSYQIEGAVDEGGRGPSIWDTFSHT
                     PGKIKHGHTGDVACDHYHRLDEDLELLGWLGVDAYRFSIAWPRVLPNGRGAVNERGLD
                     FYERLVDGLLARGIAPCVTLYHWDLPDALEGGWLSRATVDAFADYTDVVAKRLGDRVA
                     MWFTHNEPWCQAFLGYETGLFAPGHRDFAEALRCAHHLLVSHGRAVDVLRGHVRAPVG
                     IALNFVPAHSITDAPEDVAAARRFDGYFNRWFVEPVVGRGYPADMLELYRESLPEFPE
                     SDLALIARPTDVLGINYYDRVLIGQGDQGVLRVLRHQTTETPRTADREIFPEGLGETL
                     SRMHAEYGFSRLVVTENGAAFPDDMPSDGRVMDPARVEFFRNHLEQVVQARRSGVPVE
                     AYFAWSLMDNFEWSEGYTLRYGIVYVDFETQRRTPKASAHYLRSLSRGAGR"
     gene            3125..3727
                     /locus_tag="VFQ35_13615"
     CDS             3125..3727
                     /locus_tag="VFQ35_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02265 family protein"
                     /protein_id="HET9931731.1"
                     /translation="MSDNPHKQAFDLVAPCCDIADRLRDVPPSARVRGLYFRSVINVL
                     RELEHLHDYERYFPNEKWSSLALYPLGDYLLRLAVAGAVVSSPETLHQGVQQITRCNA
                     AAFANSLLGRVLVRILARDPVRVTEQGLAARRQSTTYGEWELVRKGPREIEMIYHAEY
                     MWIESAIAGAAIGSFESCETSPRIETLLRDKYNGSTVVRW"
     gene            complement(3737..5326)
                     /locus_tag="VFQ35_13620"
     CDS             complement(3737..5326)
                     /locus_tag="VFQ35_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931732.1"
                     /translation="MSEEHAPPLVILCREGERFAWLMALAERRFPGVENPVHVEVAND
                     DVREMVRRARGAHGGAPIGIVVVDEPAALLAVEAGADEAVPEEWLDEETAFGFIDRIL
                     LRARLRREQEQVRALYVHSEKLAALGTLVAGVAHEVNNPLTSLLLSAEGLKLRVNPLH
                     AALAQVEELARAKRGATPEELSEVLRIGRTGAPLAETRELLQEIESSAKNIAGVVRDL
                     KLFSRPDDDAAPEVVDIRALMDQVLRIVGRQIRQHGTLELDYASELPVVVAPSARLAQ
                     VFTNVLLNASHAISEVARPAHRIRISARADDEAVAVCISDTGPGIPPNVVGRIFDPFF
                     TTKRQGVGTGLGLCISRSILRRLGGDLLVESVHGDGATFIALIPLPDRRDLFEAYRRT
                     ANVTSQQPRPHLERRVLVVDSDERVLRALARTLENLHDVLLARDGQEAIDLLESGSRA
                     DVIVADVSMPDMSGVVLYGWLLQHRPELARRVIFVTDEDQERPSAVNETDQPVLRKPV
                     SRDALLGAVSNVFADETSPRK"
     gene            complement(5351..>6280)
                     /locus_tag="VFQ35_13625"
     CDS             complement(5351..>6280)
                     /locus_tag="VFQ35_13625"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET9931733.1"
                     /translation="FECEADRIAARYFASEAADPFLGSTRRRVAPREMTAPEAEALAA
                     EAALADAGLAASEVDAVFSWAAVPERIVPPSAPEVARRIGARAAYAVGMDAACATPLT
                     QLETAAALIESGRARHVLLTQSHLITRTFAMMHPASPNVGDAATAMVVSAAERGVPIV
                     TFAQTHGEYYSSVTWCRGKEADPPWWEAGPAYYLGSQNRDQAQELMRGTVRFAAETIN
                     QLLERAGTTLDEVDVIASVQPRKWIPEAIREALAARAAAPQTFDELAHLGGCGVITNL
                     IRAREESLLPRGGKAILYAQGAGFTRAAALVCW"
BASE COUNT         1059 a         2026 c         2076 g         1119 t
ORIGIN      
        1 tccgattcgg tgggcgccgg gatttcgatg gcggcttgca tgcgatgaac tccgtgaggt
       61 gaagacccga cttcgaccgt catgtccaaa ggccgaaggg tggcgccccg acgacatctc
      121 gagcgtttgc tcgcttgtga cgagggccgc gcagctcgag ccttcattct acacgagcgg
      181 caccttggct ctgcccgcct ctcggaaccc gagatcgtga ttcgtgggcg ttcgtgcgcg
      241 cttttctcac ccctcgcgac caacggagcg gccagcgtgt cgagccggtc cgaccgcgcg
      301 tctcgggtct cggattacgc tgttcgcgta tctttccagc gcctccgggc cggaaggccc
      361 ctgcaaggtc gtcagcttcg catgacctaa tgccgtcggt ttgacgaagg cccgtccgcg
      421 ccccgggtcg tcgcctttcg tcggagcggt gggcgagggc ttcctccgcc gaggtcccgc
      481 gcgcggccgc ccgagtgctg cgaacgtggc ccaatgtgct agtttgcgcc gaaaatgacc
      541 atagcgtatc cgtcggatcg ttccggcttc ttcttgctga gcatctgcgt ttctagctta
      601 ttggtattca cggcttgcat tcgcaagacg aactccggct gcaccaccga cgccgagtgt
      661 ggcgcgaatg cgaaatgcgc cgcgggtaac tgcgtgatga acagcgggct cggtggcagc
      721 gatgggcagg gcggcgcgtc ttcgggaggt acttccaccg cgagcggtgg caagacggag
      781 accgtgggca gcggcggcgt gggtacggga cagggcggaa aagcgactgg cggcgggggc
      841 gagatgacag cgtctggtgg cgcaatggca tccggtggga gcggcggcgg cgcggggccc
      901 aaaggcggcg cgtccggctc gggcggcggc aacgcgagcg gaggtgcgcc cccgttggtc
      961 accgaaatga tcgacgacat ggaggacggc gacgggcgga tcctgatgat gaacggccgc
     1021 caggggcctt ggcacacgtt caacagcgga gagtccaccc aaatgccggc cgggccttcg
     1081 attacccctg aaatggccgg agcaaacggc tcgatgctgg cgatgcacac gagcgggaag
     1141 gggttcgcgt acgccggcat cggcttcgac ttgagcaacg ccgacaccgc gcccgagtcc
     1201 gctcaatcga aggcgtacga tgcgtccgcg tggaccggcg tcgtattcat ggcaaaggga
     1261 gggccttcgg cgtcggcgaa gctacgcatc gagattccga tgcgcgactt cgttccgagc
     1321 gatcgcggcg ggacctgctc tggttcgtgt tggaacgttt acggctttca aatgccgatg
     1381 gcgctcggct cgagttggca agaggtcaag gtgccttttt cgtcgctcgt ccgcgaagat
     1441 ggcagcacca agcccgcgtt cgacagcaag cagatcatga gcatctcgtt caaacagcag
     1501 aaagacgatt tcgacttttg gatcgacgac gtccggttct acaaagagta ggcgatcgcg
     1561 atcgccccta tccctgacgg acccaccaac gatcgaatga tacgatgacc gtcgtttcgc
     1621 gggccgcgct cggccaagat ttcaagctcg gagtcgccac ttcttcgtac caaatcgaag
     1681 gcgccgtcga cgaaggcggc cgtggcccga gcatctggga caccttcagc cacacccccg
     1741 gcaagatcaa acacggtcac accggcgacg tcgcatgcga tcactatcat cgcctcgacg
     1801 aagacctcga gctgctcggt tggctcggcg tcgacgcgta ccgtttcagc attgcgtggc
     1861 cgcgcgtgtt gccgaacggt cgaggggccg tgaacgagcg agggctcgac ttttacgagc
     1921 gtctcgtcga cggcctcttg gcgcgcggca tcgctccctg cgtcacgctt taccattggg
     1981 atctgccgga cgcgctcgag ggcggctggc tctcgcgcgc aaccgtggac gcattcgccg
     2041 actacacgga cgtcgtcgcg aagcgcttgg gtgaccgcgt ggcaatgtgg ttcactcaca
     2101 acgagccgtg gtgccaagca ttcctcggct acgaaaccgg cctgttcgcg cccggtcatc
     2161 gcgacttcgc tgaggcgctg cgctgcgcgc atcacctact ggtctcgcat gggcgcgcgg
     2221 tcgacgtctt gcgcggccac gtgcgcgcgc ccgtcggtat cgcattgaac ttcgtgccgg
     2281 ctcactcgat cacggacgct cccgaggacg tcgcggcggc gcggcgcttc gacggctact
     2341 tcaaccgatg gttcgtcgag ccggtcgtcg ggcgtggtta cccagccgac atgctcgagc
     2401 tctatcggga gtcgctgccg gagttcccgg agtcggattt ggcgctcatc gcgcgcccca
     2461 ccgacgtact ggggatcaac tactacgacc gcgtgctcat tggccaaggt gaccagggcg
     2521 tcctcagggt tctccgccac caaacgaccg agacgcctcg caccgccgat cgggagatct
     2581 ttcccgaggg actcggggag actctgtcgc ggatgcacgc cgagtatggt ttctcgcggc
     2641 tggtcgtgac cgaaaacggc gccgcgtttc ctgatgacat gccgtccgac ggccgcgtaa
     2701 tggatcccgc gcgcgtcgag ttcttccgca atcatctgga gcaagtcgta caagcgcgtc
     2761 gctcaggcgt gccggtcgag gcatattttg cctggtcgct gatggacaac ttcgaatggt
     2821 cggagggata caccttgcgc tacggcatcg tgtacgtcga cttcgagacg cagcgccgga
     2881 ccccgaaagc gagcgcacat tacttacgct cgctttcgcg cggcgcgggc cgctgaattt
     2941 gggatcgtgc tcgcccactg aatcgagcgc gggcgcctca acgccccacg atcagagccg
     3001 ctggcgaaaa acgccaaaaa atcaggtgga agctgctgcg tcggcgagat cacgggccgc
     3061 gcgagcgtat ttgccggcgt ttcggagggg ttacgccgaa tcaaagttct gtatactgct
     3121 cgtcgtgtcg gataatccgc acaagcaggc cttcgatctg gtggcgcctt gttgcgacat
     3181 cgccgaccgc ctgagggacg tgcccccctc cgcgcgtgtg cgcgggctct attttcgcag
     3241 cgtcatcaat gtcctgcgcg agctcgagca cctgcacgac tacgagcggt acttcccgaa
     3301 cgagaagtgg tcgtcactcg cgctctaccc actcggagac tatctcttgc ggctcgcggt
     3361 tgcgggggcg gtcgtctcgt cgccggagac gttgcaccag ggcgttcagc agatcacgcg
     3421 gtgcaacgcc gccgccttcg cgaacagcct cctcggtcgc gtgctcgtgc gcatcttggc
     3481 gcgcgacccg gtgcgcgtca ccgagcaggg gctcgcggcg cgccggcaat cgaccacgta
     3541 cggcgagtgg gagctcgttc gtaaagggcc gcgcgagata gagatgatct atcacgcgga
     3601 gtacatgtgg atcgagtctg ccatcgccgg tgccgcgatc ggcagcttcg agagctgtga
     3661 gacgtcgccg cggatcgaga ccctgcttcg cgacaagtac aacggctcga ccgtcgttcg
     3721 ctggtgacgg cgcgcgctac ttacgcggcg acgtttcgtc cgcgaagacg ttgctgaccg
     3781 cgccgagcaa ggcgtcgcgc gaaacgggct ttctcagcac gggctggtcg gtctcgttga
     3841 cagccgacgg ccgctcctgg tcttcgtcgg tgacgaagat gacgcgccga gcgagctcgg
     3901 gccggtgctg caagagccat ccgtagagca cgacccccga catgtccggc atcgatacat
     3961 cggccacgat aacgtctgcg cgcgagcccg actcgagcaa atcgatcgcc tcttgtccgt
     4021 cgcgcgcgag cagcacgtcg tgcaggttct cgagggtacg tgcgagcgcc cgcaacaccc
     4081 gctcgtccga gtccacgacg agaacgcgcc gctcgaggtg aggccgcggc tgttgggacg
     4141 tgacgttggc ggtgcgccga tacgcctcga agaggtcgcg ccggtcgggt agtggaatga
     4201 gggcgatgaa cgtggcgccg tcgccgtgca ccgactcgac caacaagtca cctccgagcc
     4261 tgcgcaagat cgaccgggag atgcataagc cgagtccggt gccaaccccc tgtcgtttgg
     4321 tcgtgaagaa cgggtcgaag atgcgaccca ccacgttcgg ggggatgccg gggccagtgt
     4381 ccgaaatgca aactgcgacc gcctcgtcgt cggcgcgggc cgaaatccga atgcgatggg
     4441 ctgggcgcgc cacttcgctg atggcgtggg acgcgttcaa gagcacgttc gtgaagactt
     4501 gcgcgaggcg cgcgctcggc gcgaccacca ccgggagctc cgaggcgtag tcgagctcga
     4561 gcgtgccgtg ctgacggatc tgccgtccaa cgatgcgcag cacttggtcc atcaaggcgc
     4621 ggatgtcgac cacctcgggc gcggcgtcgt cgtccggccg agagaagagc ttgaggtcgc
     4681 gcacgacccc cgcgatgttc ttcgctgacg actcgatctc ttgcagcagc tctcgagtct
     4741 ctgcgagcgg tgctccggtg cgtccgatgc gcaggacctc cgagagctct tcgggtgtgg
     4801 cgccgcgctt ggctctcgct agctcttcta cctgcgcgag cgccgcgtgc agcggattga
     4861 cgcggagctt caggccctct gccgagagca gcagcgaggt gagggggtta ttgacctcgt
     4921 gggcgacgcc ggccaccaac gttccgagcg cggcgagctt ttccgaatga acgtagaggg
     4981 ctcgcacctg ctcctgctca cggcgcaggc gcgcacgcag caagatgcga tcgatgaaac
     5041 cgaacgcggt ttcctcgtcc agccactctt ctggtacagc ctcatccgcg cccgcctcga
     5101 ccgccaagag cgcggccggc tcgtcgacga ctacgatgcc gatcggcgct ccgccgtgag
     5161 ctccgcgcgc gcgacgaacc atctcgcgca cgtcgtcgtt cgcgacctcg acgtgcaccg
     5221 gattttcgac gccgggaaac cgcctctcgg ctagcgccat gagccaggcg aagcgctccc
     5281 cttcacggca aaggatcacc aaaggtgggg cgtgttcctc gctcatggct gctcgaaggc
     5341 agtccacgtt ctaccagcaa acgagcgcag ccgcgcgagt aaatccagca ccttgtgcgt
     5401 agaggattgc cttgccgccg cggggtagga gtgactcctc ccgagcgcgt atgaggttcg
     5461 ttattacgcc gcaaccccct aggtgtgcca gctcatcgaa cgtttggggt gcagctgcgc
     5521 gcgcagcgag ggcctcgcga atggcctctg ggatccattt gcgaggctgg acgctcgcga
     5581 tgacgtcgac ctcgtcgagc gtcgttcccg cacgctcgag tagctgattg attgtttcag
     5641 ccgcgaatcg cacggttccg cgcatcagct cttgggcctg atcacgattc tgactaccga
     5701 gatagtacgc gggccccgct tcccaccaag gaggatccgc ctctttaccg cggcaccatg
     5761 tcaccgaaga gtaatactcg ccatgcgtct gcgcgaacgt gacgatcggt acgccgcgtt
     5821 cggccgccga aacgaccatc gcggtggctg cgtcacctac attgggcgac gccggatgca
     5881 tcatcgcaaa ggttcgagtg atgaggtgac tctgcgtgag caggacatgc cgcgcccggc
     5941 ccgactcgat cagcgcggcc gcggtttcga gctgcgtcag cggcgtcgca caagcggcgt
     6001 ccatccccac cgcgtaggcg gcgcgcgccc cgatgcgccg ggccacttcc ggcgcgctcg
     6061 gcggaacgat gcgctcgggg accgcggccc acgagaacac cgcgtccact tccgatgcag
     6121 cgagccccgc gtcggcgagc gcggcttcgg ccgcgagcgc ttcggcttcc ggcgcggtca
     6181 tctctcgggg agcgacgcgt cggcgcgtgg agccgagaaa tggatccgcg gcttcgctcg
     6241 cgaagtagcg agcagcgatg cgatcggctt cgcactcgaa
//
LOCUS       DASTKS010000660         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_774531, whole genome shotgun sequence.
ACCESSION   DASTKS010000660 DASTKS010000000
VERSION     DASTKS010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774531"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1007
                     /locus_tag="VFQ35_13630"
     CDS             162..1007
                     /locus_tag="VFQ35_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9931734.1"
                     /translation="MQPPPSEREPTEAAPFPAEPALASASGAPAKGGSSAIRGGLLAI
                     FLSLLAKGKGLLIGLKALPFAKVLLTGGSMVASVLAYAMNGGVAFAVGLVLMILVHEL
                     GHGAAMKRAGVDASWPIFIPFFGAMISMKGRPEHPRVEAAIAYGGPLAGTGAALVAAA
                     LGLMLHSPFLLALSYTGFFLNLFNMAPFAFLDGGRIARVLSRKSWIVGAIIMGGMCLM
                     SPSPQLVMIAGMGAMHAFRRDNSDLELVTEQDRQTWAFRYFGLCGFLALAILFTHQLT
                     RAHGW"
     gene            1017..1460
                     /locus_tag="VFQ35_13635"
     CDS             1017..1460
                     /locus_tag="VFQ35_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931735.1"
                     /translation="MTVDYQGYQAAPTRFSRSDQSDLNTLAVCHYVYAALLAVGGLIP
                     LGISLVAVGFFSAMVPSHEPALKGLVGGALLLIWGAIGLLLWLKAGFVAYSGLSLRRG
                     QHRTLSIVVACLCCLNMPLGTLLGVFTLIVLNRPAVRAGYESLPP"
     gene            complement(1492..>2176)
                     /locus_tag="VFQ35_13640"
     CDS             complement(1492..>2176)
                     /locus_tag="VFQ35_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9931736.1"
                     /translation="LVSHGHLDHIGAIAQHAARRSLMKMSEGVYLVPRSIQPAVERLF
                     NAAGELDGHPIARTIVPLDPGEEFQLTGGRFVRPFTTYHRVPSQGYTIWERRHRLRKE
                     FQGRPGVELGRLRAQGVTLDEPHDAPVLAFTGDTRIEVLERTPELERVDTLVMETSFV
                     DERVSVSEAREMGHIHLDEVSQRHELVQSRDVVFTHFSARYGGADIARAISRLPEGLR
                     ERVRPFPAS"
BASE COUNT          327 a          701 c          709 g          439 t
ORIGIN      
        1 gttccgcgag caagctccgc ctcttgcatc gctagccgca tccaccgctc gtgctcagga
       61 gacatcgggg ggcgagtata atcgggaagc gtcagcgccg cttcgctact cgacttaccg
      121 gcttgcggag cggtggagag cgcatcatcg tgtaggtctc aatgcaacca cctccttccg
      181 agcgggagcc gaccgaagcg gcgccttttc ctgccgagcc ggccctcgcg agcgcgtcgg
      241 gcgctcctgc aaagggcggt agctccgcaa tccggggcgg cctgcttgct atttttctga
      301 gcctgctcgc gaagggcaag ggccttttga tcgggctgaa agcgcttccg ttcgcgaaag
      361 tgctcctgac cggtggcagc atggtcgcgt ccgtcctcgc ctacgccatg aacggcggag
      421 tggcgttcgc cgtcgggctc gtcctcatga ttttggtgca tgagctcggt catggcgccg
      481 ccatgaagcg tgccggcgtc gacgcgagtt ggcccatctt catcccgttt ttcggcgcca
      541 tgatctcgat gaagggcagg ccagagcatc ctcgcgtaga agcggcgata gcctacggcg
      601 gaccgctcgc cggcaccggg gcggcgctgg ttgcggcggc cctcggtctc atgctccaca
      661 gccccttcct gctcgcgctc tcttataccg ggttctttct gaatctgttc aacatggcgc
      721 cgttcgcctt cctcgacgga gggcggatcg cgcgggtgtt gtcgcgcaag tcttggatag
      781 tgggagccat catcatgggc ggcatgtgct tgatgagccc gtcgccgcaa ctcgtcatga
      841 tcgctggcat gggcgcgatg cacgcgtttc gccgagacaa ttcggatctc gagttggtca
      901 ccgagcagga ccgacaaacc tgggctttcc gctactttgg cctgtgcggt ttcttggccc
      961 tggcgattct tttcacgcac caactcaccc gcgcgcacgg ctggtaagct cctgccgtga
     1021 cggtggatta tcagggttat caggcggcac cgacccgctt ctcgcgatcg gaccagagcg
     1081 atctgaatac gctcgcggtg tgtcactacg tgtatgcagc gctgctggcg gtcggcgggt
     1141 tgattcccct cggcatatcg ctcgttgcag tcggattttt ttcggcgatg gtgccctcgc
     1201 acgaacccgc tctcaagggt ttggtggggg gagcgctgct gctcatttgg ggtgcaattg
     1261 ggctgctctt gtggctcaag gcgggcttcg tcgcgtattc ggggctgagc ttgcggcgtg
     1321 ggcagcaccg gaccctgagc atcgtggtcg cgtgcttgtg ctgcttgaac atgccgctcg
     1381 gcacgctgct cggcgtgttc acgctcatcg tgctgaaccg ccccgccgtg cgcgccggct
     1441 acgagtcttt gccgccgtag ctccaagtgg cgcggcgcgc ttgcctggcg ctcagctcgc
     1501 cggaaaagga cgcacccgct cgcgcagccc ttcgggcagc cgcgaaatcg ctcgggcgat
     1561 gtcggcgccg ccgtaacgag ccgaaaagtg cgtgaatacg acgtcacggc tctgcacgag
     1621 ctcgtggcgc tgactgacct cgtcgaggtg gatgtgaccc atctcgcgcg cttcggacac
     1681 gctgactcgc tcgtcgacga agctcgtctc catcacgagc gtatcgacgc gctcgagctc
     1741 tggagtgcgc tcgaggacct cgatgcgagt gtcccccgtg aacgcgagaa ctggcgcatc
     1801 gtgcggctca tccagcgtga cgccctgcgc gcgcaaccgg ccgagctcga ctcctggccg
     1861 cccctggaac tctttgcgca gccggtgccg gcgctcccaa atcgtataac cctggctcgg
     1921 aacgcggtga tacgtcgtga agggccgcac gaagcgaccg cccgtcaatt ggaactcctc
     1981 gccgggatcg agcggaacga tggtgcgcgc aatcggatgc ccgtcgagct caccggccgc
     2041 gttgaacagg cgctcgaccg caggctgaat actgcgcggg acgaggtaca cgccctcgga
     2101 catcttcatc agcgagcgtc gcgcggcgtg ctgcgcaatc gcgccgatgt gatcgaggtg
     2161 cccgtggctg acgagc
//
LOCUS       DASTKS010000661         7157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1222195, whole genome shotgun sequence.
ACCESSION   DASTKS010000661 DASTKS010000000
VERSION     DASTKS010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222195"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1368
                     /locus_tag="VFQ35_13645"
     CDS             232..1368
                     /locus_tag="VFQ35_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931737.1"
                     /translation="MAVVRRDGREPGLPSLNEARVERRRAFVMGKVRMPAVPMHFWLW
                     AAVLLSASGVVYWRLAEGKLETERSKIMAKQRAMSKALGPRLFPFREKSEGWVRELAA
                     PWTGPLVSPSADLEKVRNAGTIYLRLRMANAKGERDMRKAAKDSLRDGFTSCLFVTRS
                     SSGTNSTVAASAAPAASVAPAAMGAAAAGAACRTIGDCASGLLCNEWNTCAPPEQPYN
                     MRLVYRTLRVLSSDWTDELHEASSELALSAYDRDLENVARRDVPVTVEILARSKFFAL
                     VLDEDPPGGIPKPAPESGESDAETLQRLPHFARIGIWDIASGTPLLRVRAVAAGEVVQ
                     MGGGAAKDAVTRTATERQVNSCALALKAREALEQHTVADDAALP"
     gene            complement(1456..3759)
                     /gene="clpA"
                     /locus_tag="VFQ35_13650"
     CDS             complement(1456..3759)
                     /gene="clpA"
                     /locus_tag="VFQ35_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET9931738.1"
                     /translation="MKVSPEVEIACNLALKEAERRRHDVMTVEHLLFALLHDDRTAKV
                     LKKSGGNVEKLKARLDKILRDELPAVPAGGGLVTPTPSRGFQRVLQRAAFHVQQSGKE
                     RLEGHNVLIAIFAEPESLAAEAMKAEGITRFDVVNFVSHGVAKDGSGDSGLELSESEE
                     ARERDDDDEGGDESQGDALSQFAVNLNERARNGDIEPLVGRDKEIRRAIQVLCRRKKN
                     HPLFVGDAGVGKTAIVEGLAALIEAGKAPKALEGATIFALDMGGLVAGTRFRGDFEKR
                     VKAVLKQIEKLKGSILFVDEIHTVIGAGAASGGTLDAANLLKPALSSGKLRCIGATTF
                     EEYRTHIERDRALARRFQKIDVNEPTVEETIQILQGLQKRYEEFHEVTYTAEAIKGCA
                     ELADRHLRDKKLPDKAVDLLDESGADTKLDLGPKSVVDIGRVETVVSRMAQIPPRQVS
                     HDDKAALRNLEGDLEKVVFGQDRAIRELSTAIKLARAGLRAPNKPIGSYLFTGPTGVG
                     KTEVARQLAATLGIELTRFDMSEYMERHTVSRLIGAPPGYVGYDRGGLLTEAVAKTPH
                     CVLLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGKKADFRHVILIMTSNVGAADLARAP
                     VGFGSRESKGRDDPAFKNTFSPEFRNRLDARIAFDPLTPAVMNRVVEKFVKELNAQLG
                     EREVTFELSDEARDYLATKGYDKDNGARPLARLIQDEVKRPLTEELLFGELEFGGRVL
                     VQVRDGKLHFDLKGNPKPGAPAPRLLN"
     gene            complement(3756..4094)
                     /gene="clpS"
                     /locus_tag="VFQ35_13655"
     CDS             complement(3756..4094)
                     /gene="clpS"
                     /locus_tag="VFQ35_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET9931739.1"
                     /translation="MATDRPKHDESDGDLATDERRKVERPRKYVVVLHNDDYTTMEFV
                     VHVLMKFFHLGETESTQIMLHVHHKGYGIVGAYTRDVAETKSAQVMDYAKEHGHPLRC
                     TAEPEGFGES"
     gene            complement(4420..4560)
                     /locus_tag="VFQ35_13660"
     CDS             complement(4420..4560)
                     /locus_tag="VFQ35_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931740.1"
                     /translation="MGTPQDPRTRRRTRIRRAKKNLEWEQKRARENAEAAKPKASAKT
                     AT"
     gene            complement(4634..5656)
                     /locus_tag="VFQ35_13665"
     CDS             complement(4634..5656)
                     /locus_tag="VFQ35_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET9931741.1"
                     /translation="MTNIVEPTRLTKKDFETDQDVRWCPGCGDYAILATVQRVMPELG
                     ILPENMVWVSGIGCSSRFPYYMNTYGFHTIHGRAPAFATGIKVANPDLSVWLVTGDGD
                     GLSIGGNHLMHCLRRNVDVKILLFNNRIYGLTKGQYSPTSEFGKVTKSTPFGSADQPV
                     DPVSFALGANASFVARSIDTEAAHLADVLRRAAKHSGASFTEIYQNCNVFNDGAFDVI
                     KEKPQNVEHQLRVEHGKPLLWGKDRDRGLKLNAHTFKLEAVRVGENGVTLDDILVHDE
                     TNPTLAYMLSRLSFPEFPVALGVLYAAPRPTYDGTLREQRTTIDAQKGKPDLQRLLRG
                     GETWEI"
     gene            complement(5696..>7157)
                     /locus_tag="VFQ35_13670"
     CDS             complement(5696..>7157)
                     /locus_tag="VFQ35_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET9931742.1"
                     /translation="LENGSLSNYRLLSIDITKLTQNAVKDSGLSNKESNRCKNFWTLG
                     LMFWIYGRPLEPTLRWIDQKFGKRAEVAAANSAALRAGHAYGETAEIFHSRYEVPAAP
                     VERGVYRNISGNTATALGLVAAAQLSGRELVLGAYPITPASDVLHELSKHKHFGVTTL
                     QCEDEIAAVCAAIGASYAGSIGVTSTSGPGMALKTEAIGLAVSTELPLVLIDVQRGGP
                     STGLPTKTEQADLLQAVYGRNSEAPVCVIAAATPGDCFYMAIEAVRLAVKYMTPVILL
                     TDGYLANGAEPWKIPDVAQLPKIEVKFRTEPEGFHPFLRDETTLARAWAIPGTPGLIH
                     RIGGLEKDFNSGHISYSPDNHERMSLVRAAKIAGIANDIPDQTIEVGNDHGKLLVVGW
                     GSTYGAIREAVERCRERGLDVSHAHVRNINPFPKNLGDLLRRFDRVLVPELNLGQLVR
                     LLRSQFLVPAESFPKIQGQPFKIAELEERIRKALES"
BASE COUNT         1111 a         2214 c         2439 g         1393 t
ORIGIN      
        1 gtggtcaaga gcgtgttcca gacggcggcg ttcgcgggtt acccgaacgt cagcttcatg
       61 gtgggtcgcc tcggcaagca gggcggctga gcccgacgct cctcccgcag tagttcgtag
      121 acattgcgcc cggtggcaac gccaagttgc cgtcgggcgt tgtcgttagt agggcttcga
      181 gcgcggctcg tcgaatcggc gaaccgcggc taggcgaaga cacgagagta catggcagta
      241 gtgcgcagag acgggcgaga accaggcctg ccttcgttga acgaggcgcg cgtggagcgg
      301 cggcgcgcct tcgtcatggg gaaggttcgc atgccggccg tccccatgca tttttggctg
      361 tgggcggcgg tgctgctgag cgcttcggga gtcgtgtact ggcgactcgc cgagggaaaa
      421 ctcgagacgg agcgcagcaa gatcatggcg aaacagcgcg ccatgtccaa ggcgctcggc
      481 ccgcgcttgt ttccgtttcg cgaaaagagc gaagggtggg tgcgcgagct cgcggcgccg
      541 tggaccggac cactcgtttc accttccgcc gatctcgaaa aggttcgcaa cgccgggacg
      601 atctacctgc gccttcgcat ggcgaacgcc aagggcgagc gtgacatgcg caaagcggcg
      661 aaggactctc tgcgcgacgg ctttacgtcg tgcctgttcg tcacgcgctc gagctcgggc
      721 acgaattcga cggtagcggc ctccgcagca cccgctgcgt cggtggctcc ggcggctatg
      781 ggtgccgcgg ccgcgggcgc tgcctgccga accatcggtg actgcgcttc tggcttgctc
      841 tgcaacgagt ggaacacctg cgcgcctccc gagcagccct acaacatgcg gctcgtctat
      901 cgcacgctgc gcgtgctctc cagcgactgg accgacgagc tccacgaagc ctcgagcgaa
      961 ctcgctcttt cagcgtacga ccgagacctc gagaacgtgg cgcgccgcga cgtgcccgtc
     1021 acggtcgaga tcctggcgcg cagcaagttc ttcgcgctgg tgctcgacga ggatccgcct
     1081 ggtggcattc cgaagcccgc acccgagtcg ggcgagtccg atgccgagac gctccagcgc
     1141 ctcccgcact tcgcgcgcat cggcatctgg gacatcgcga gcggtacgcc gctcctccgc
     1201 gttcgcgccg ttgctgcggg cgaagtcgtt cagatgggcg gtggcgctgc gaaggacgcc
     1261 gtgacgcgaa ccgcgaccga gcgccaagtc aatagctgtg cgctcgctct caaggcgcgc
     1321 gaggcgctcg agcagcacac cgtcgctgac gacgccgcgc tgccctgaat cagacggctc
     1381 gcaccgctcg cgcgagcacc gtcgcaaggc gcgcgagaat tacttcgacg cgcccagcgg
     1441 cactcgcggc tagccctagt tgaggagacg cggtgccggt gcgcccggct tggggttgcc
     1501 cttcaagtcg aagtgcagct tgccgtcgcg cacctgaacc agcacgcggc cgccaaattc
     1561 gagctcaccg aacaagagct cttcggtgag cgggcgcttg acctcgtctt gaatgagacg
     1621 cgcgaggggg cgggcgccgt tgtccttgtc gtagcccttg gtcgcgaggt agtcgcgggc
     1681 ctcgtcgctg agctcgaagg tgacttcgcg ctcgccgagc tgggcgttca gctccttcac
     1741 gaacttctcg acgacgcgat tcatcaccgc gggcgtgagt ggatcgaacg cgatgcgtgc
     1801 gtcgagccgg ttcctgaatt cggggctgaa cgtgttcttg aacgccggat cgtcgcgccc
     1861 tttgctctcg cggctgccga agccaaccgg cgcgcgggcg agatcggccg cgcccacgtt
     1921 gctcgtcatg atgaggatga cgtgccggaa gtcggctttt ttgccgttgt tgtcggtgag
     1981 ggtgccgtga tccatcacct gcaagagcac gttgaagacg tcggggtggg ccttctcgat
     2041 ttcgtcgagc aagagcacgc agtgtggggt cttcgcgacg gcttcggtca atagacctcc
     2101 gcggtcgtaa ccgacgtagc cagggggcgc gccgatcagg cgagatacgg tgtggcgctc
     2161 catgtactcg ctcatgtcga agcgagtcag ctcgatgccg agcgtcgccg cgagctggcg
     2221 tgccacctcg gtcttgccga cgccggttgg gcccgtgaac aggtacgacc cgatcggctt
     2281 gttcggcgct cgcaggcccg cgcgagcgag cttgattgcg gtggagagct cgcgaatcgc
     2341 tcgatcttga ccgaagacga ctttctcgag gtcgccctcg aggttgcgaa gcgccgcctt
     2401 gtcgtcgtgg ctcacctggc gcggggggat ctgcgccatg cgcgagacga ccgtctcgac
     2461 gcgaccgatg tcgacgacgg acttgggccc gaggtcgagc ttggtgtcgg cgccggactc
     2521 gtccaggagg tcgacggctt tgtccgggag tttcttgtca cgcaggtggc gatcggcaag
     2581 ctccgcgcag cctttgatcg cctccgcggt gtacgtgact tcgtggaact cctcgtagcg
     2641 cttctgcaag ccctggagga tttgaatcgt ctcttcgacg gtgggctcgt tgacgtcgat
     2701 cttttggaag cgacgggcga gggcgcgatc gcgctcgatg tgcgtccgat actcctcgaa
     2761 ggtcgttgcg ccgatgcaac gcagcttgcc cgacgagagc gccggcttga ggaggttcgc
     2821 ggcgtcgagc gttccgccgc tcgcggcgcc cgcgccgatc acggtgtgaa tctcgtcgac
     2881 gaacaggatc gagcctttga gtttctcgat ttgtttgagg acggctttga cgcgcttctc
     2941 gaaatcgccc cggaagcgcg tgccagcgac gagcccgccc atgtcgagcg cgaagatggt
     3001 ggcgccttcg agggccttcg gggccttccc ggcttcgatg agcgcggcca aaccctcgac
     3061 gatggccgtc tttccgacgc cggcgtcgcc gacgaagagg ggatggttct tcttccggcg
     3121 gcaaaggact tggatggcgc gacgaatctc cttgtcgcgc cccacaagcg gctcgatgtc
     3181 tccattgcgc gcacgctcgt tcaagttgac ggcgaactgc gagagcgcgt caccctggct
     3241 ctcgtctccg ccctcgtcgt cgtcgtcgcg ctcacgcgcc tcttcgctct cactgagctc
     3301 gagcccgctg tcgccgctgc catccttggc gacaccgtga gaaacgaagt ttacgacgtc
     3361 gaaacgcgtg atcccttcgg ctttcatcgc ttcggccgcc aggctttcgg gctcggcgaa
     3421 gatggcgatg agcacgttgt gaccttcgag acgttctttc ccgctttgct ggacgtgaaa
     3481 ggccgctcgc tggagcacgc gctggaagcc ccgagacggc gtcggcgtca cgaggcctcc
     3541 gcccgcgggc acggcgggca gctcgtcgcg caagatcttg tcgaggcgag ccttgagctt
     3601 ttcgacgttg ccgcccgact tcttcagcac tttggcggtt cggtcgtcgt ggagcagggc
     3661 gaagagcaag tgctcgaccg tcatgacgtc gtgacggcgt cgctcggcct ctttgagggc
     3721 caggttgcag gcgatttcga cttccggact aaccttcacg attccccgaa tccttccggt
     3781 tccgccgtgc agcgcaacgg atgaccatgt tctttggcgt agtccatcac ctgcgccgac
     3841 ttggtttcgg ccacgtcgcg cgtataggca ccgacgatgc cgtagccctt gtggtgcacg
     3901 tgcagcatga tttgcgtcga ttcggtctcg ccgagatgga aaaacttcat caacacgtgc
     3961 acgacgaact ccatggtggt gtagtcgtcg ttgtgcaaga ccacgacgta cttgcgcgga
     4021 cgttcgactt tgcgccgttc gtcggtcgcg agatcgccat ccgattcgtc gtgcttgggg
     4081 cggtccgtgg ccatgcccac gaaatttgag cgcgtcggag cctgctgtcg agccccccat
     4141 tcggaggggc agcaaaccgc gccaatacgc gctaccgcga catacgagtt cgtttcggca
     4201 cgcgaatgcc ggcgctattg ttcatccgag gcggccggat ttcggaccgg ccagcgtcgt
     4261 tcgccgcgcc cggggttgcc ccgggatcga ggccgttcgc gcgcggcggc cggttgcagc
     4321 cggaccgtgc gacggccata gaggcctcgg cgggcgcgcg tcgagcatcg attttgttcg
     4381 aagcgacgtt ggcggccgag gccagctacc gattgctaat caggtcgcgg tcttggccga
     4441 agccttgggc tttgctgcct cggcgttctc gcgggcgcgc ttctgctccc attcgaggtt
     4501 cttcttggcg cgacggatgc gagtgcggcg acgggtacgg gggtcttgcg gggttcccat
     4561 ggtggcgcgt cctactagca ggtttgttcg cgactgccaa cggcggcatt ctgccgaacg
     4621 gacaggagaa agctcagatt tcccaggttt cgccgccgcg cagcaagcgc tggaggtcag
     4681 gcttgccctt ttgggcgtcg atggtggtcc gctgctcgcg caaagtgccg tcgtaagtgg
     4741 ggcggggagc cgcgtagagc acgccgaggg cgaccgggaa ctcgggaaag ctgaggcgcg
     4801 agagcatgta cgcgagggtc gggttggtct cgtcgtgcac gagaatgtcg tcgagggtga
     4861 cgccgttctc gccgacccgc accgcttcga gcttgaacgt gtgcgcgttc agcttgaggc
     4921 cgcggtcccg gtctttgccc cagagcaacg gctttccgtg ctcgacgcgg agctggtgtt
     4981 cgacgttttg aggcttctct ttgatgacgt cgaaggcgcc gtcattgaag acgttacagt
     5041 tttggtaaat ctcggtgaac gaggcgccgc tgtgtttggc ggcgcgtcgc agaacgtcag
     5101 cgaggtgagc ggcctcggtg tcgatggaac gcgcgacgaa ggaggcgttg gcgccgagcg
     5161 cgaagctcac cggatcgacg ggttgatctg cggagccgaa cggcgtgctc ttcgtgactt
     5221 tgccgaactc gctcgtcggc gagtactggc ccttcgtgag gccgtaaatt cggttgttga
     5281 agagcagaat cttcacgtcg acgttgcgac gcaagcaatg catcaggtga ttgccgccga
     5341 tgctgaggcc atcgccgtcc ccggtgacga gccacaccga gaggtccggg ttcgcgacct
     5401 tgatgccggt tgcgaacgcg ggcgcgcgtc cgtgaatcgt gtgaaacccg tacgtgttca
     5461 tgtagtacgg gaagcgactg ctgcagccga tgccgctcac ccagaccatg ttctcgggca
     5521 aaatcccgag ctcgggcatc acgcgctgga cggtcgcgag gattgcgtag tctccgcagc
     5581 ccgggcacca gcgtacgtcc tggtcggtct cgaagtcttt cttggtgagg cgagtcggtt
     5641 cgacgatgtt cgtcatggcg cgattctctc gagaaatcgg tggggtgggc tcgagtcagc
     5701 tctcgagggc tttgcggatg cgctcttcga gctccgcgat cttgaatggc tgcccctgaa
     5761 tcttcgggaa gctctcggca gggacgagga actggctgcg cagcaggcgc accagctgtc
     5821 cgaggttcag ctcggggacg agcacgcggt cgaaacggcg cagcaggtct ccgaggttct
     5881 tcgggaatgg attgatgttg cggacgtggg cgtgcgaaac gtcgagcccg cgctcccggc
     5941 agcgttcgac tgcctcgcgg atcgcgccgt aggtcgagcc ccaaccaacg accaagagtt
     6001 tgccgtggtc gttgccgact tcgatggtct ggtccgggat gtcgttcgcg atgccggcga
     6061 tcttggcggc gcgcacgagt gacatgcgct cgtgattgtc cggcgaatac gagatgtggc
     6121 cagagttgaa gtctttttcg aggccgccaa tgcgatggat gaggccgggc gtaccgggga
     6181 tcgcccacgc gcgagcaagc gtcgtttcgt cgcgcaagaa ggggtggaag ccctccggct
     6241 ccgtgcggaa cttcacttcg atcttcggga gctgggcgac gtctgggatc ttccagggct
     6301 cggcgccgtt ggcgagatag ccgtcggtga ggaggatgac cggggtcatg tacttcaccg
     6361 cgaggcgcac ggcctcgatt gccatgtaga agcagtcgcc cggagtggca gccgcgatca
     6421 cgcacaccgg ggcttcgctg tttcgaccgt acaccgcctg gagcaagtct gcttgttcgg
     6481 tcttcgtcgg gagccccgtc gaagggccgc cgcgctgaac gtcgatgagg acgagcggca
     6541 gctccgtcga caccgcgagc ccgatcgcct cggtcttcaa cgccatgccc gggccgctcg
     6601 tgctggtcac gccgatgctg cccgcgtacg aggcgccgat cgccgcgcaa actgccgcga
     6661 tctcgtcctc gcattggagc gtcgtgaccc cgaagtgctt gtgcttgctg agctcgtgca
     6721 gcacgtcgct cgcgggcgtg atgggatacg cgccgagcac gagttcgcgt ccgctgagct
     6781 gcgcggcggc gacgaggccg agcgcggtcg cggtgttgcc gctgatgttg cggtagacgc
     6841 cgcgctccac cggcgccgcg ggcacctcgt agcggctgtg gaagatctcc gcggtttcgc
     6901 cgtaagcatg gccggcgcgc aacgccgcgc tgttcgcggc cgctacctcg gcgcgcttgc
     6961 cgaacttctg gtcgatccaa cgcagcgtgg gctcgagcgg gcgcccgtag atccagaaca
     7021 tcaacccgag agtccagaag ttcttgcagc ggttgctctc cttgttcgaa agcccgctgt
     7081 ccttcaccgc gttttgcgtg agcttcgtga tgtcgatcga gagcagccga taattcgaga
     7141 gcgacccgtt ctcgagc
//
LOCUS       DASTKS010000662         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1892597, whole genome shotgun sequence.
ACCESSION   DASTKS010000662 DASTKS010000000
VERSION     DASTKS010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892597"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFQ35_13675"
     CDS             complement(<1..280)
                     /locus_tag="VFQ35_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931743.1"
                     /translation="MSQPLLYLPAVSRALSAERLGAYRYRGDDDVGAVSTYLWSVRLC
                     ETLYPALHGLEVALRNSLFNVGEQRFAGVAHREVPCWLDAHPPILAPDE"
     gene            complement(550..1029)
                     /locus_tag="VFQ35_13680"
     CDS             complement(550..1029)
                     /locus_tag="VFQ35_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931744.1"
                     /translation="MSNSRLAAGALALGLAIFGCAAELDRSQDYDWAYADSAGGSGNG
                     GSGNPGGGMTSVPDDPQCLKTIITNQCATCHSASSAASLGKGLDLTSSNIGQRLVNKD
                     SACDGDPKIINTSDKNNSAFLLRVTGAAKGCSKLVMPPGGALMGADLQCVQEWINKF"
     gene            complement(1236..2687)
                     /locus_tag="VFQ35_13685"
     CDS             complement(1236..2687)
                     /locus_tag="VFQ35_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET9931745.1"
                     /translation="MVCESGGALTLSLFTAAREAPTALALSTGTSKLCYDELAERVER
                     VARSLWSRRIVGDSEPVAVVARPTLASIEVLHALVATAAPIVLVHPSLPAVEQNALAE
                     RSGARSLLRPDDEEFGLEERAPWPGPTGARSDDAALAIVPTSGSTGQPKLVVLSQGAF
                     AASARASAANIPLAPGDRWLMCLPPAHVGGFSILTRSLLAKSAVVLFDPGPRGLLRAL
                     PELAERLLDEQVTVLSLVPAVLDALLTTLPDWRPPPSLRAVLIGGAAVPESLLARARA
                     RAVPLLTTYGLTEACSQVTTTRRGEIPSVRDGVVSSGFALPGVELEVRDRGRICVRSP
                     ALCSGYLGATAPIDENGWFETEDRGYIDERGELYVLGRISELIVSGGENVDPLRVEAA
                     LTSCPGVLAALVFGVPDERYGEVVACCLIRGEGFDLGRVSATLRQRLSRFELPRRLAV
                     VEELPTLPNGKVDRPKARAFFADRLQPFEHPRS"
     gene            complement(2660..3712)
                     /gene="menC"
                     /locus_tag="VFQ35_13690"
     CDS             complement(2660..3712)
                     /gene="menC"
                     /locus_tag="VFQ35_13690"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9931746.1"
                     /translation="MKLERSVARKHLRFAAPFANAHQRWEERETLALTLTDERGFSAQ
                     GEAAPLPGFSRDTLAEAERGLLEFPLELAAAIVERSVSDPSCTLRELESCAAIASPAA
                     RFAFESALVELIAARSGAMPSDLLGGPSSSSPSALPLARLVDVAHAEEEVAAALTTGF
                     TTIKLKLGTTLGFARELELIARVRARFGSAWGLRLDANQSFPRDEALSRLRALLPFDP
                     EFVEEPTRPDETPTEASPVPIALDESLTSPAFLVPSALLARNVRVLVLKPTLLGGIAV
                     TRAFVERARAVGLDWVLSHTFESALGYRALKMLAFALPPSRLAHGLGPHAALDEPAAA
                     LGVRAGKLWFARAEAP"
     gene            complement(3709..>3943)
                     /locus_tag="VFQ35_13695"
     CDS             complement(3709..>3943)
                     /locus_tag="VFQ35_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931747.1"
                     /translation="AYGTPLWLFATRSLAPTVLLPWVTAPLALRLIRGVVREQGRALN
                     PLLVLTAKLLFFFGVLFALGIVLAAQAHSGIAA"
BASE COUNT          698 a         1271 c         1369 g          605 t
ORIGIN      
        1 actcgtccgg cgccaggatc ggcgggtgcg cgtcgagcca gcacggcacc tcgcggtgcg
       61 cgactcccgc gaagcgctgc tcgcccacgt tgaacaggct gttgcgcagc gccacctcca
      121 gcccgtgcag cgcggggtag agcgtctcac acaggcgcac gctccacagg taggtggaca
      181 cggcgccgac gtcgtcgtcg ccgcggtagc ggtacgcgcc caagcgctcg gcggagagcg
      241 cgcgcgacac cgcggggagg tagagcaggg gttgactcat gggctgaccg gggttatcat
      301 ggttgcactg tccccgggtt aatcctctgc gcaagcatga tcccggggtc tttttttgcc
      361 ctgctcccgg gtgagcggag agaacagcgg cggctttcgc ggcttttgcg ggtttcggct
      421 tcgcgtcggc gagcccggga atcagcgtga gtggaacgat tgcaagtatg gtgagcgtgt
      481 aacggagcgg ggctcgcatc ttcatcgtcg gttctgctcg tcagaaggcc actgtccgct
      541 ggtcgcgaac tagaacttgt tgatccactc ttggacgcac tgcaggtcgg cgcccatgag
      601 agcgccgcca gggggcatca ccaattttga acagccctta gcggcgccgg tcactcgcag
      661 caaaaacgcg ctgttgttct tgtccgaggt gttgatgatc ttcggatctc catcgcaggc
      721 cgagtccttg ttgacgaggc gttgtccgat gttcgaagac gtgaggtcga gccctttgcc
      781 cagcgaagcc gcgctcgaag cggagtggca cgtcgcgcat tggttggtga tgatcgtctt
      841 cagacactgc gggtcgtccg ggaccgaagt catcccgccc ccaggattgc cggaaccgcc
      901 gttgcccgag ccgcccgcgc tgtcagcgta ggcccagtcg taatcttggg atctgtcgag
      961 ctccgcggca cagccgaaga tggccaaacc gagcgccagc gcgcccgccg ccaaacggga
     1021 attgctcatc ttgcgaacgt ggagcgactg tcttttggac atcgggaggc ttcgggcacc
     1081 ctagcatcaa acatccgcta gtggcggttg cgaacgaaga actccgcagc aaaaacggga
     1141 ggatatgctg cgcctcgcac gtgttcgcta gatttttttg ccgtgtttgg atcgcgcttc
     1201 gcctgccgcc aatgactcgc gtttcgcgaa atatttcagc tacgcggatg ctcgaagggc
     1261 tgaaggcgat cggcgaaaaa cgcgcgtgct ttgggccggt cgaccttacc gttcgggagc
     1321 gtcggcagct cctcgacgac agcgaggcga cgcggcagtt cgaagcgcga gagacgttgt
     1381 cgcagcgtcg cgctgacccg acccaggtcg aagccttcgc cgcgaatcag gcagcacgcg
     1441 acgacttcgc cgtagcgctc gtcgggcact ccgaagacca aggctgccag cactcctggg
     1501 cacgaagtga gcgcagcttc gacgcgcaac gggtccacgt tctcgccgcc gctcacgatg
     1561 agctcgctga tgcgaccgag cacgtagagc tcgccgcgct cgtcgatgta gccgcgatct
     1621 tcggtttcga accagccatt ttcgtcgatg ggcgcggtcg ctccgagata gccggagcag
     1681 agtgcgggcg agcggacgca gattcgccct cggtcgcgga cttctagctc gacgcccggc
     1741 agagcaaagc cggagctcac gactccgtcc cgaacgctcg ggatctcacc gcgccgtgtg
     1801 gtcgtcacct gcgagcaagc ttcggtgaga ccgtaggtcg tgaggagcgg cacagctcgg
     1861 gcgcgagctc gcgccaaaag cgactcgggc accgccgcgc cgccgatcaa gacggcacgc
     1921 agcgagggcg gggggcgcca atctggcagc gtcgtgagca gcgcatcgag gaccgccggg
     1981 acgagagaaa gcacggtgac ttgctcgtcc aaaagtcgct ctgcgagctc aggcaatgcc
     2041 cgcaacagcc cgcgaggacc tggatcgaac aaaacgacgg cactcttcgc gagcaggctg
     2101 cgggtcagga tcgaaaagcc accgacgtgc gccggcggaa ggcacatgag ccagcgatca
     2161 cctggtgcga gcgggatatt cgcggcgctc gcgcgcgccg aagccgcgaa cgcgccttgg
     2221 ctcagcacga cgagcttcgg ttggccagtg ctacccgagg tcgggacgat cgcgagcgcc
     2281 gcatcgtcag agcgcgcacc ggtggggcca ggccaaggtg cgcgctcttc gagcccgaac
     2341 tcttcgtcgt cgggccgaag gagagagcgc gcgcccgagc gctcggcgag cgcgttctgc
     2401 tcgacggcgg gtaacgatgg gtgcacgagc acgatgggcg ccgccgtcgc gacgagcgcg
     2461 tgcaggactt cgatgctcgc gagggtcggg cgagcgacca ccgcgacagg ctcagaatca
     2521 ccgacgattc ggcgagacca caacgaacga gcgacgcgtt cgactcgctc cgcgagttcg
     2581 tcgtagcaaa gcttggaagt gcccgtgctc aacgccaacg ccgtcggcgc ctcgcgtgcg
     2641 gccgtgaaga gcgagagcgt cagggcgcct ccgctctcgc aaaccacagc ttgcctgcgc
     2701 gcaccccgag cgcagccgcc ggctcgtcga gcgcggcgtg cggaccgagg ccgtgagcga
     2761 gtcggctggg aggcagcgcg aacgcgagca ttttgagcgc ccgatacccg agcgcgctct
     2821 cgaaggtatg agagagcacc cagtcgagtc cgaccgcgcg cgcacgctcc acgaaggccc
     2881 tggtcacggc gatcccgccg agcagagtcg gcttcaaaac gagcacgcgg acgttccgcg
     2941 cgagaagcgc gctcggcacg agaaacgccg ggctcgtcag cgattcgtcg agggcgatcg
     3001 ggacgggcga ggcctcggtc ggcgtttcgt cggggcgggt cggctcttcg acgaactctg
     3061 ggtcgaatgg caggagtgcg cgcaaccgag agagcgcttc gtcgcgcgga aagctctgat
     3121 tggcgtctag ccggagaccc caggccgaac cgaagcgcgc tcgcacgcga gcgatgagct
     3181 cgagctcccg cgcgaagccg agcgtggttc cgagcttcag cttgatcgtc gtgaaaccgg
     3241 tggtcagtgc cgccgcgacc tcttcttcgg cgtgcgcgac gtcgacgagc cgagcgagcg
     3301 gcagcgcgct cggggagctc gagctcggtc caccgagcaa gtcgctgggc atcgcgccgc
     3361 tgcgcgcggc gatgagctcc accaacgccg actcgaaagc aaagcgcgcc gcgggtgacg
     3421 cgatcgcggc gcaactttcg agctctcgga gcgtgcacga cgggtcgctc acgctgcgct
     3481 cgacgatcgc ggccgcgagc tcgagcggaa actcgaggag cccgcgttcc gcctcggcca
     3541 gcgtgtcgcg tgagaatccg gggagggggg cggcttcgcc ctgggcgctg aacccgcgct
     3601 cgtcggtcaa ggtgagagcg agcgtctcgc gctcttccca gcgctggtgc gcgttggcga
     3661 acggcgccgc gaagcgcagg tgtttacggg cgacgctgcg ctcgagcttc acgccgcgat
     3721 gcccgagtgc gcctgcgccg cgagcacgat cccgagcgcg aagagcactc cgaaaaagaa
     3781 caggagttta gcggtgagga ccaagagcgg gttcagcgct cgtccctgct cccgcaccac
     3841 cccgcggatc aacctgagcg cgagcggcgc cgtgacccac ggcaggagca cggtcggcgc
     3901 gagagaacgc gtagcgaaca gccaaagggg cgtcccgtaa gcg
//
LOCUS       DASTKS010000663         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_774839, whole genome shotgun sequence.
ACCESSION   DASTKS010000663 DASTKS010000000
VERSION     DASTKS010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774839"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..919
                     /locus_tag="VFQ35_13700"
     CDS             98..919
                     /locus_tag="VFQ35_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9931748.1"
                     /translation="MSDTPTADLRSARDQFLAKFTRGARITEEFLKEYENLAQRAQAL
                     ETENAILRAQVEADEAMRELVRKIEQLEQEKAELLSRFRRVEQQSTSVSARVEEVELE
                     FANLANLFVASNQIHSSLSPRGVTRRIKEVLAQFVGAERYAMYLVNPSGSELVPIASE
                     GVPGNKLLPVRVDATRVGEVFLSGNVVVNEDIDPSQGTLDEPCAVIPLSIDERTVGAV
                     AIFSTLSQKTRFDTVDFELFKLLGQHAAAALVSASLFVQADRKLPGLEAFLDLSV"
     gene            924..1301
                     /locus_tag="VFQ35_13705"
     CDS             924..1301
                     /locus_tag="VFQ35_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931749.1"
                     /translation="MADYSCLIVEDSPMMRQLLVFALARVKNLRVTEADDGVDGLRKL
                     ASARYDIIVTDINMPIMDGLKLVKRVRSDPVHKDTPIVIITTEGSQEDRQRALQLGAN
                     AYITKPIQAPQVIATVKELLRIE"
     gene            1298..1936
                     /locus_tag="VFQ35_13710"
     CDS             1298..1936
                     /locus_tag="VFQ35_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9931750.1"
                     /translation="MSERPELWVKICGVTREEDARAAVAAGASALGLNFVTRSKRYVD
                     VALARRLADAVRGEIELVGVVADEPVERIRELMLEVGLDSVQLHGGEPPEFLQALPKA
                     FKAIGIEGPSDVSAAQAFVGARLLVDAKSAGVTGGTGHAFDWRLVEALARRRPIILAG
                     GLRPDNVADAVRQVAPYGVDVAGGVELEGEPRHKDADKIKRFIENARNAVQR"
     gene            1940..2416
                     /locus_tag="VFQ35_13715"
     CDS             1940..2416
                     /locus_tag="VFQ35_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931751.1"
                     /translation="MRRALLLPWPLLLACAGPHLRGASAIEREKEPHGSERATLVTRD
                     CRDERGAPAAQLDLELVYLDPSGGPRRLLERRTDHDSVLVENTYEDGGAHVFVYVSAD
                     VKHRDVLHRVRLAPDGMSGELSFSRDFSVKDSEKGFRAETSRIALRCALVPLEARE"
BASE COUNT          507 a          826 c          880 g          431 t
ORIGIN      
        1 gagaggtgat aggctgaacc tcggtcgctt caccgcgcac tggggcgaag cgggcggcaa
       61 ctagagcatg accgacgaaa agcgtaatcg ttcggatgtg agcgacactc cgacggccga
      121 cctgcgcagc gcgcgcgacc agttcctcgc gaagttcacg cgcggcgctc gcatcacgga
      181 agagttcctc aaggaatacg aaaatctcgc gcagcgcgcg caggcgctcg agaccgaaaa
      241 cgcgattttg cgcgcgcagg tcgaggccga cgaagcgatg cgcgagctcg tgcgcaaaat
      301 cgaacagctc gagcaagaga aggccgagct gctctcgcgc ttccgccgcg tcgagcagca
      361 gagcacgtcc gtctcggccc gggtggaaga ggtcgagctc gagttcgcca atctcgcgaa
      421 cctgttcgta gcgagcaacc aaatccactc gagtctgtca ccccgaggcg taactcgccg
      481 gataaaagaa gtgctcgcgc agttcgtcgg cgccgaacgc tatgcgatgt acctcgtgaa
      541 cccgagcggc agcgagctcg tgccgatcgc gtcggagggc gtgcctggta acaagctcct
      601 gccagtgcgc gtcgacgcga cccgcgtagg cgaggtattc ctttcgggta acgtcgtcgt
      661 gaacgaagac atcgacccga gccaaggcac attggatgaa ccctgcgccg tgatcccgct
      721 ctctatcgat gagcgcacag tgggcgcggt tgcgattttt tctactcttt cccaaaaaac
      781 gcgcttcgac acggtagact tcgagctctt caaactcctc ggccaacacg ctgcggctgc
      841 gctggtgagc gcgagcctat tcgtccaggc cgatcgcaag ctgcccggat tggaagcgtt
      901 cttggatctg agcgtttaga accatggccg attactcctg cctcatcgtt gaagactcgc
      961 cgatgatgcg tcagctgctc gtgtttgcgc tggcgcgcgt caagaacctc cgggtcaccg
     1021 aagccgacga cggtgtcgac gggctccgta agctcgcgtc cgcgcgctac gacatcatcg
     1081 tcaccgatat caacatgccc atcatggatg ggttgaagtt ggtgaaacgg gtgcgcagcg
     1141 atccggtcca caaagacacg ccaatcgtca tcatcaccac cgagggctcg caagaggatc
     1201 gacagcgcgc cctgcagctc ggcgcgaacg cctacatcac gaagccgatt caggcgccgc
     1261 aggtgatcgc gaccgtcaaa gagctgctcc gcatcgaatg agcgaacgtc ccgagctgtg
     1321 ggtgaagatc tgcggagtca cccgagaaga agacgctcgc gcggcggttg cggctggcgc
     1381 gagcgccctt gggttgaact tcgtaacgcg ctcgaagcgc tacgtcgacg tcgcgttggc
     1441 gcgccgcctg gccgacgccg tgcgcggcga aatcgagctg gtaggtgtcg tcgccgatga
     1501 gcccgtcgag cggatccgcg agctcatgct cgaagtgggc ctcgactcgg tccagctcca
     1561 cggcggggag ccgccggagt tcttgcaggc cctgcccaag gccttcaaag cgatcggcat
     1621 tgagggcccc tcggacgtga gcgcagcgca ggcgttcgtg ggcgctaggt tgctcgtaga
     1681 cgcgaagagc gccggcgtga cgggcggcac ggggcacgct ttcgattggc gattggtcga
     1741 ggcgctcgcc cgtcgacgcc ccatcatcct cgcgggtggg ctgaggcccg acaacgtcgc
     1801 ggacgcggta cgccaggtcg cgccgtacgg cgtggatgtc gccggtggcg tcgagctgga
     1861 gggcgagccg cgccacaagg acgcagacaa gataaaaagg ttcatcgaga acgcgcgcaa
     1921 cgccgttcaa cgctgatcga tgaggcgcgc cctgctgctg ccgtggccat tgctgctcgc
     1981 gtgcgccggg ccgcacctgc gtggagcaag cgcaatcgag cgagagaagg aaccgcacgg
     2041 ctccgagcga gcgaccctcg tgaccaggga ctgccgcgac gagcgcggtg cgcccgcggc
     2101 ccagttggat ctcgagctcg tttacctaga cccgagcggc gggcctcgac ggctcctcga
     2161 acgtcgcacc gaccacgaca gcgtgctcgt cgagaacact tacgaggacg gcggcgcgca
     2221 cgtgttcgtt tacgtgtccg ctgacgtcaa gcaccgcgac gtgcttcacc gcgtccggct
     2281 cgcaccggac gggatgagcg gtgagctctc gttttcgcgc gacttttccg tgaaggacag
     2341 tgaaaagggc tttcgcgcgg agacatcgcg catcgcgctc cgctgcgcct tggtgccgct
     2401 cgaagcgcgc gagtgagccc gctccgcgga acctgggcca cttcgaaaaa taactattcg
     2461 acgaggttca gggcgctggt ctagggggtg tcccaagttc ctgcccgtcg cgagagccaa
     2521 gaaacgcgag cgagaagcgc gcccgaggag tgagcacagg aggaaggctg aagcctttcg
     2581 aggagcggag cgacgaggcc gtgcttcgca gccgcaggat cttggctcgc agcagggcgg
     2641 ggaa
//
LOCUS       DASTKS010000664         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_329357, whole genome shotgun sequence.
ACCESSION   DASTKS010000664 DASTKS010000000
VERSION     DASTKS010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329357"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFQ35_13720"
     CDS             complement(<1..629)
                     /locus_tag="VFQ35_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931752.1"
                     /translation="MQAPADVASIDELEAHAQAALEWQTLLDRIAEGTRSEPGRFRVK
                     SLQPSASAAEARSRMARVRALLELGEHGVELPLSEFPDILETLSRASVGASASGSDFL
                     DVLRVLEQAAALRGVAKEHAEQAPELAAVLDSDAKLTRVAERLASCIERDGNVSDGAT
                     PALAEARQRAREVREELKRRLGDLTQRYADVLSGTYYTERDGRYVLPVRS"
     gene            complement(682..1140)
                     /locus_tag="VFQ35_13725"
     CDS             complement(682..1140)
                     /locus_tag="VFQ35_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931753.1"
                     /translation="MQNATLVVIEFGASWPRWLSPTSADHMAVVAQHYEGSAHSLITQ
                     VSSRLSRLVNTGWRIEDAILVSNGRTDIEAIAARAVLVRGLLQHLRDVSGSRLLLSVK
                     KEFGQRPGHTLASLSQALRENALGTGIALGIRVGEEAPRYLNGAIPRLAS"
     gene            1307..2269
                     /locus_tag="VFQ35_13730"
     CDS             1307..2269
                     /locus_tag="VFQ35_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931754.1"
                     /translation="MRIYRLSWLLAVGCFLLGARGSAQGQGAFPTQSAPAPSTPTPTP
                     APAPPRAVPPPAAPAAPPSAPVAPAPPPVGGAPVAPPGTVPPPPPEPGAPVNPPAAPP
                     VVPPPPAPPPPSSAVLVEPLAPAPPPVPAPDPTVHRHDGFYLRIGLGGGYFGAKVEPE
                     VGTLHATGGGPAFEFALGGTIADGVVIGGGLYTASVNRLKWSGDPLASDVRGGAGSLA
                     LLGPFVDYYPNPKDGFHIQGSLGITGLNFEEHASTVPPKEWSGQGGGLMFGAGYEFWV
                     GSQWSIGGIARVLLVSATMREKNSDTDFSGKAFAPSILFVATHH"
     gene            2364..>3886
                     /locus_tag="VFQ35_13735"
     CDS             2364..>3886
                     /locus_tag="VFQ35_13735"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9931755.1"
                     /translation="MTHRHPLLSSFELPQSGAPSCGRRTVGRSALAGLLLAVSPLTVA
                     CSGSDDSGPRELTPGRLAQFEKDLDAARDHLEVPGAAYAIISHGAVVAANGIGVRNLT
                     TGEAVSTDTLFRVGALTKAMSSALVASRVDEGAVAWDSLAESIDPNFKLSDDALTVSV
                     TLQELLGMATGIGSSPPFWWEYQTADDVIEATSTAAKAGARGSFVYNDEMFARGVYLG
                     VGAGGETRPLLEAYSAELQARVFGPLEMSPAQVSDDPSQVGADFATSYTLSMVEQVNF
                     KTEATPFAFGGLAPSGGVVTSVNEYAKFLIAEMQGGVGPNGGRIATEENLKKTQLAQV
                     ALPTGTLARYTMGWVRDRDAGRTVLWHDGSIDGYRSYAFFLPDDQIGLVVLTNGYNGD
                     RLGAIARALLTNTLYGGSSFASTSFQAEYDSDKSALVASMTKIRPQFTTDPYVPYLGA
                     YEHGLSLVQHDDETLWLEAPGVSGRLLDASALSAPGVLLVGNGQFVLNLLQFVGNGSG
                     "
BASE COUNT          614 a         1330 c         1233 g          709 t
ORIGIN      
        1 gagcgcaccg gcagcacgta gcgcccgtca cgctccgtgt aataggtgcc gctcagtacg
       61 tccgcgtagc gctgcgtcag gtcaccgagt cgtcgcttca gctcttcacg aacttccctc
      121 gcgcgctgac gagcttcggc gagggccggt gttgcgccgt ccgacacgtt gccgtcccgt
      181 tcgatgcagc tcgcgagacg ctcggcgacg cgggtcagct tcgcgtcgct gtcgagcacg
      241 gccgcgagct ccggcgcttg ctcggcgtgc tccttcgcga cgccccgcag cgccgctgcc
      301 tgctcgagca ctcgcagcac gtccaaaaag tcgctcccgc tcgcgctagc tccgacgctc
      361 gcccgcgata gcgtttcgag gatgtccggg aactcggata ggggcagttc gacaccgtgc
      421 tctcccaact cgaggagcgc gcggacgcgg gccattcgcg aacgggcctc cgccgcgctg
      481 gcagacggtt gcaagctctt cacccggaaa cgcccgggct cactgcgcgt cccttcggcg
      541 atgcgatcga gcagagtttg ccactcgagc gccgcttgtg cgtgcgcttc tagctcgtcg
      601 atcgaggcca cgtcggccgg ggcttgcatg gcgcgctttg tagcactggc cctggacctc
      661 gcacctaccc gacgcgaagg ctcagctcgc gagccgtgga atggcgccgt tcaaatacct
      721 aggcgcctcc tcacccactc ggatgccgag cgcgatgccg gtccccaatg cattctctcg
      781 gagcgcctgc gacaaggacg ccaacgtgtg gccgggacgc tgaccgaatt cctttttgac
      841 actcagcagc aagcgggaac cggacacgtc acgcagatgc tgcaacagcc ctcgcaccaa
      901 gaccgcacgc gcggcgatgg cctcgatatc ggtgcgtccg ttggagacca gaatcgcgtc
      961 ctcgatacgc cagccggtgt tcacgagccg cgacaaccgg ctcgacactt gggtgatgag
     1021 ggagtgtgca gagccttcgt agtgctgtgc cactacggcc atgtgatcgg cgctcgtcgg
     1081 gctcaaccag cgaggccagc tggcgccgaa ctcgatgacc accagcgtcg cgttttgcat
     1141 tggcagggat cctccgagct ttccgtccga gccatacggg cgtctgcgga gctgggccaa
     1201 caaacctcat ctttgcgccg cccgatgagg gaagccctca gccaacaaac cccatacccc
     1261 ggttggacca gaaccgagag acgcgataac gtcttcgttc gtagcgatgc ggatttatcg
     1321 tctttcgtgg ctgctcgcgg tcggctgttt tctcctcggc gctcgcggtt cggcgcaagg
     1381 tcaaggcgcg tttccgacgc agagcgctcc ggcgccttcc acacctacgc ccacacccgc
     1441 tcccgctccg ccgcgagccg ttccgccgcc agcagctccc gcggctcctc cgtccgcgcc
     1501 agtagctccc gctccacctc cggttggggg cgccccagtt gctcctccgg gcacggttcc
     1561 cccgcctccg cccgagccgg gcgcgccggt gaacccgcct gcggcgccgc cggtcgtgcc
     1621 cccgccgcct gctcctcctc ctccttcgtc cgcggtgctc gtggagccgc tcgctccggc
     1681 gcctcccccg gtgccggcgc ccgatcccac ggtgcatcga cacgacgggt tctacctgcg
     1741 catcggcctc ggcggcggtt acttcggcgc gaaggtcgag cccgaggtgg gcacgctgca
     1801 tgcgaccgga ggtggaccag ccttcgagtt cgcgctcggt ggaacgatcg ctgatggagt
     1861 cgtgatcggc ggcggtctct atacggcgag cgttaaccgt ttgaagtgga gcggcgaccc
     1921 tctagcctcg gacgtgcgcg gaggcgcggg ctccctggcc ctgctcgggc cgttcgtcga
     1981 ctattacccg aaccccaaag acggctttca cattcagggc tcgctgggca tcacggggct
     2041 caacttcgaa gagcacgcct cgaccgttcc tcccaaggag tggtcagggc aaggcggcgg
     2101 gctcatgttc ggcgcgggct acgagttctg ggtgggctct cagtggagca tcggcggcat
     2161 cgcccgcgtc ttgctcgtga gcgccaccat gcgagagaag aacagcgaca cggatttttc
     2221 aggcaaggcc ttcgcgccat caatcttgtt cgtcgcgacg catcattgag ctgagttcct
     2281 gcaaaaatct gccaaagtta cgggttgttt cgcgtcgtcg cttctcgatt cacctgcgct
     2341 cgccgttggg gtatgggcag cgggtgacgc atagacaccc gcttctctcc tccttcgagc
     2401 ttccacaatc cggcgcgccc tcgtgcggac gccgcaccgt ggggcgcagc gctctggcgg
     2461 gacttttgct cgcggtttct ccactgacgg ttgcgtgctc ggggtcggac gactccggtc
     2521 cgcgcgagct aacccccggc cgcctcgctc aattcgagaa ggacctcgac gcggctcgag
     2581 atcatctgga ggtgccgggc gcggcgtacg ccattatctc gcatggagcg gtcgtggcgg
     2641 cgaatggcat cggcgtacgc aacctcacca ccggtgaagc ggtcagcact gatacgctgt
     2701 tccgggtcgg cgcgctcacg aaagccatgt cgtcagcgct cgtcgcgagc cgagtcgacg
     2761 agggcgcggt ggcgtgggat tcgctggcgg agtcgatcga tccgaacttc aagctgagtg
     2821 acgacgcgct caccgtgagc gtcacgctgc aggagttgct cggaatggca accggcatcg
     2881 gctcctctcc gccattttgg tgggagtacc agaccgcgga cgacgtcatc gaagccacgt
     2941 cgaccgccgc gaaggcgggc gcgcgcggtt cgttcgttta caacgacgag atgttcgcgc
     3001 ggggcgtcta cctgggcgtc ggcgctggcg gcgagacacg accgctgctc gaggcttaca
     3061 gcgccgagct gcaagcgcgc gtcttcggtc ccctcgagat gtcgcccgcg caagtctccg
     3121 atgatccatc ccaagtcggg gccgacttcg cgacctctta cacgctttcg atggtcgagc
     3181 aggtgaactt caagacggag gccacaccgt ttgcattcgg tgggctcgcg ccgtctgggg
     3241 gggtcgtcac tagcgtcaac gagtacgcca agttcttgat cgcggagatg caaggcggag
     3301 tcggtccgaa cggcggtcgc atcgccacgg aagagaacct caagaagact cagctggcac
     3361 aggttgcgtt gccgaccggc accctggccc gatacaccat gggatgggtg cgcgatcgag
     3421 acgcggggcg caccgtgctt tggcacgacg gttcgatcga cgggtaccgc agttacgcct
     3481 tctttctacc cgacgatcaa atcggcctag tcgtcctcac gaatggctac aacggtgacc
     3541 gcctcggggc gattgctcgc gcgctactca ccaacaccct ctatggcggc agctctttcg
     3601 cgtcgacctc gttccaagcc gagtacgact cggacaagag cgccctcgtg gcgagcatga
     3661 cgaagattcg cccgcaattt acgaccgacc cctacgtgcc gtacctgggc gcctacgaac
     3721 acggcttgag cctggtccaa cacgacgacg agacgctttg gctcgaagct ccgggcgtca
     3781 gcggtcgatt gctcgacgct tcggcgctca gcgccccggg cgtgctgctc gtcggcaatg
     3841 ggcaattcgt gctcaacttg ctgcagttcg tcggcaacgg cagcgg
//
LOCUS       DASTKS010000665         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1446361, whole genome shotgun sequence.
ACCESSION   DASTKS010000665 DASTKS010000000
VERSION     DASTKS010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446361"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFQ35_13740"
     CDS             complement(<1..900)
                     /locus_tag="VFQ35_13740"
                     /inference="COORDINATES: protein motif:HMM:NF040140.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931756.1"
                     /translation="MQIRKILALKGPNIWGNFPCIEAWVDLGKFEDFPSNTLPGFNDR
                     IMQWLPTMVEHRCSIGERGGFFQRMRTGTWLGHVLEHVTIELQSLSHMPAGYGRARET
                     SERGVYKVVVECEDHRFTETCMRAAHGLIMAAVDGKEVDVAGEIRKLREAADQLCLGP
                     STQAIVSAAEARKIPAIRLTEGNLVQLGYGKAQRRIWTAETARTSAVAEAIAQDKELT
                     RKLLSSVGVAVPSGRVATSREDAWAAAESLGLPVVVKPQDANHGRGVSIRLEDRAAVE
                     AAFDYAAKEGSGVVVERFIPGTQY"
     gene            1171..>2787
                     /locus_tag="VFQ35_13745"
     CDS             1171..>2787
                     /locus_tag="VFQ35_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9931757.1"
                     /translation="MDDALRYASQLVVLTNRRVYVQSQGGTWHSETLGENSKLAHREH
                     GGVGELRLLQAGHGVMRFRHTLAEAEGLQAFTQAFEELTLAGAKERRPRLDAQPDDVD
                     LPVPSTRMPLLRLLAFARPHLPKVVLGFALTLSTTAVGLIPPYLTMPLVDKVLIPGQA
                     GQLAADPALRQVAVYLGGLGFAAFIAWLLAWAQGAVLANVTERISADLRNRTFAHLQR
                     LSLEYFGGKRTGDLIARISTDSEHLCSFLSDTLIDFITDVLMITGTATVLISLDPLLA
                     LAALISFPPIAWLIVRIRGRLSHGFLRGGRVWSAMTNILADTIPGIRVVKAFSQERRE
                     VERFARANERIVDVNNRINALWTFFWPLVALLNQVGLLVVWAVGAYQVLHHRVTVGVL
                     TAFVAYISRFYTRVESMSRMLTATQRASAGAQRLFEILDRVPTVLDPKDPLPLPQVRG
                     EVRFERVSFRYGSRLVLNDVSFTVKPGQLVGIVGHTGSGKSTIANLLCRFYDATSGAV
                     LLDGVDLRRFSIEEYRKHISIVLQDPFLFFG"
BASE COUNT          453 a          927 c          888 g          519 t
ORIGIN      
        1 gtactgagtg ccggggatga agcgctcgac gacgacgccg ctgccctctt tcgcggcgta
       61 atcgaaggcg gcctcgaccg cggcgcgatc ttcgagacga atcgatacgc cgcggccgtg
      121 gttcgcgtcc tgtggcttca ccacgacggg gagcccgagg gactcggcgg cggcccaggc
      181 gtcttcacgg ctcgtggcaa cacgacccga tggcacggcg acgccgaccg aggagaggag
      241 cttgcgggtg agctccttgt cttgcgcgat cgcttcggca acggcgctcg tgcgcgccgt
      301 ctcggcggtc cagatacggc gctgtgcctt gccgtacccg agttgaacca agttgccctc
      361 ggtcagccgg atggcgggga tcttacgcgc ctcggcggcg ctcacgatgg cttgcgtgct
      421 cgggccgaga cacagttgat ccgcggcctc gcgcaacttg cggatctcgc ccgcgacatc
      481 cacctctttt ccgtcgactg cggccatgat caggccgtga gccgcgcgca tgcacgtctc
      541 ggtgaagcga tggtcctcgc actcgaccac cactttgtag acgccccgct cgctcgtttc
      601 gcgggcgcga ccgtacccag ccggcatgtg gctgagtgac tggagctcga tggttacgtg
      661 ctcgagcaca tgaccgagcc aagtgccggt acgcatgcgc tggaaaaaac cgccgcgttc
      721 gccgatgctg catcgatgct ccaccatcgt gggcagccat tgcatgatgc gatcgttgaa
      781 cccgggtagc gtattggaag ggaagtcctc gaactttccg aggtcgaccc aggcctcgat
      841 gcaggggaaa ttgccccaaa tgttgggccc tttgagcgcg agtattttgc gaatctgcat
      901 ggtgggggag cgcgaaggcc gcgcggcgcc gcatcctgag ccaagccggc cggcggcgcc
      961 agcgcgctgt tggcggctcg aaaatcggcc aatagggggc caacttgcgt tcgaagagcg
     1021 ttcggctcaa cgtccgcggc ccccgggcct cgacgcgcgg ggaacgtgct catgagtgct
     1081 cattcggaac gcaatgcggt cgagacaggc tccttcgagg gtctcgaact gagagcggac
     1141 gagaaagttc aagcagtatt tcggcctgac ttggacgacg ccctccgtta cgcgagccag
     1201 ctcgtcgtct tgacgaatcg gcgcgtctac gtgcagtcgc aggggggcac ctggcacagc
     1261 gagaccctcg gcgaaaattc gaagctcgcg catcgggagc acggcggggt cggtgagctc
     1321 cgtctgttgc aagcgggaca tggggtcatg cgcttccggc acactctcgc cgaagcagaa
     1381 ggactacagg cgttcacgca ggctttcgaa gagctcacgc tcgccggcgc caaagagcga
     1441 cgtccgcgcc tcgacgcgca gcccgacgac gtcgaccttc ccgtcccgag cacgcgcatg
     1501 ccgctgctgc gtttgttggc gtttgcgcgg ccgcacctgc ccaaggtggt gctcggcttc
     1561 gcgctcacgt tgtcgacgac ggcggtgggg ctgattccgc cgtacctcac gatgcctctc
     1621 gtcgacaagg tgctcatccc cgggcaagcc ggacagcttg cggcggaccc ggccctacga
     1681 caagtcgcgg tctatctcgg tggtctcggg ttcgcggcgt tcatcgcgtg gctcctcgcc
     1741 tgggctcaag gcgcggtcct cgcgaacgta acggagcgga tcagcgcgga cttgcgcaac
     1801 cgaaccttcg cgcatctgca gcgcctgagc ctcgagtact tcggaggcaa gcgcacgggt
     1861 gatctcatcg cgcgcatcag caccgatagc gagcacctgt gttcgttcct gtccgacacg
     1921 ctgatcgact tcatcaccga cgtcttgatg atcaccggca cggccacggt gctcatcagc
     1981 ctcgacccgc tcttggcctt ggcggcgctg atctccttcc cgcccatcgc ctggctcatc
     2041 gtgcgcattc gcggtcggct atcacacggc tttttgcgtg gcgggcgagt gtggtcggcg
     2101 atgaccaaca tcttggccga caccatccct ggcattcgcg tcgtcaaagc gttctcgcaa
     2161 gagcgccgcg aagtcgagcg ctttgctcgc gccaacgagc gcatcgtcga cgtcaacaac
     2221 cgcatcaacg ccctgtggac cttcttctgg ccgctcgtcg cgctgttgaa ccaggtcggc
     2281 ctgctcgtgg tgtgggcggt aggcgcgtat caagtgctgc accaccgcgt caccgtcggc
     2341 gtgctgaccg ccttcgtggc ctacatcagc cgattctaca cgcgagtcga gagcatgagc
     2401 cgcatgctca ccgccactca acgcgcgtcg gccggcgcgc agcgcctgtt cgaaatcctc
     2461 gatcgcgtgc cgaccgtgct cgacccgaag gatccgctgc cgctccccca ggtgcgcggc
     2521 gaagtacgct tcgagcgagt ctcgtttcgc tacggcagcc gcctcgtttt gaacgacgta
     2581 agcttcacgg tgaagccggg tcagctcgtc ggcatcgtcg gccacaccgg ctccggcaag
     2641 agcaccattg cgaacctact ttgccgcttt tacgacgcga cgagcggcgc ggtgttactc
     2701 gacggcgtcg acctccggcg cttctcgatc gaggaatacc gcaagcacat cagcatcgtg
     2761 ctccaggacc cgttcttgtt cttcggc
//
LOCUS       DASTKS010000666         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_106974, whole genome shotgun sequence.
ACCESSION   DASTKS010000666 DASTKS010000000
VERSION     DASTKS010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106974"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFQ35_13750"
     CDS             complement(<1..591)
                     /locus_tag="VFQ35_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9931758.1"
                     /translation="MSNDSRELLGRAERLLPGGVNSPVRAFRAVGGGPVFVKSARGSK
                     LTSAEGREYIDYVGSWGPAILGHAHAKVVEAVQRAAESGLSFGAPTELEVRFAERIIE
                     IYPSIEMLRCVSSGTEATMSALRVARGFTKRNVIVKFDGGYHGHSDSLLVAAGSGAAT
                     FGNPDSAGVPASIVEHTATLSYNDVPALEQLFARRGS"
     gene            complement(593..1864)
                     /gene="glyA"
                     /locus_tag="VFQ35_13755"
     CDS             complement(593..1864)
                     /gene="glyA"
                     /locus_tag="VFQ35_13755"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9931759.1"
                     /translation="MPSPNHLTDPPLSTRDPEIARLIKEEEVRETVKLRLIPSENYVS
                     RAVLEASGSVLTNKYSEGYAGKRYYEGQQIIDQVEELACSRVKALFGVDHVNVQPYSG
                     SPANLAVYLAFCQPHDVVMGLALPAGGHLTHGWNVSITGKYFKSVAYGVSRQDHRIDF
                     AEVRALAREHKPKLIWCGTSAYARTLEFDKFREIADEVGAKLCADIAHISGLVVGGAH
                     PSPAGIADIITSTTHKTFRGPRGGMILCKSEYAKDIDRAVFPGLQGGPHNHTTAGIAV
                     AAKEAATPEFKQYAHNVVANAKALAEGLLGHGFSLVTGGTDNHLVLIDLTGKNISGKI
                     AAKALDRAGIVCNYNAVPFDQRKPFDPSGIRIGTPAITSRGMGLPEMKKLAGWMNQVV
                     SHADDAALLDRIAAEVKEMCSGFPPPGITVS"
BASE COUNT          365 a          693 c          719 g          389 t
ORIGIN      
        1 gctgccgcgc cgcgcgaaga gctgttcgag cgcagggacg tcgttgtagg agagggtggc
       61 ggtgtgctcg acgatgctcg ctgggacacc cgccgaatcg gggttgccga aggttgccgc
      121 accgctgccc gcagcgacca ggagcgagtc cgaatggccg tggtacccgc cgtcgaactt
      181 cacgatcacg ttgcgcttcg tgaagccgcg cgcgacgcgc agcgcgctca tcgtcgcttc
      241 ggtgccgctc gacacgcagc gcaacatctc gatgctggga taaatctcga tgatgcgctc
      301 ggcgaagcgc acctcgagct cggtgggggc gccgaagctg agtccgctct cggcggctcg
      361 ctgcaccgct tcgacgacct tcgcgtgggc atgtcccaat atcgctggtc cccacgagcc
      421 tacgtagtcg atgtactcgc gaccctcagc gctcgtgagc ttcgacccgc gcgcgctctt
      481 cacgaacacg ggtccgccgc ccaccgcacg gaacgcgcgc acgggactgt tcactccgcc
      541 ggggagcaac cgctccgcgc gcccgagaag ctctcgcgag tcgttcgaca tttcagctca
      601 ccgtgatgcc agggggaggg aagcccgagc acatttcctt cacctcggcc gcgatgcgat
      661 cgaggagcgc cgcgtcgtcg gcgtgcgaaa cgacttggtt catccagccc gcgagcttct
      721 tcatctcggg gaggcccatg ccgcgcgacg tgatcgccgg tgtgccgatg cggatgccgg
      781 acggatcgaa cggcttgcgc tggtcgaacg gcacggcgtt gtagttgcaa acgatgccag
      841 cacgatcgag cgccttcgcg gcgatcttgc cggagatgtt cttgcccgtg agatcgatga
      901 gcacgaggtg gttgtcggtg ccgccggtga cgagcgagaa gccgtgtccg agtagccctt
      961 cggcgagcgc cttcgcgttc gcgaccacgt tgtgcgcgta ctgcttgaac tcgggagtgg
     1021 ccgcttcttt ggcggcgacg gcgataccgg ccgtcgtgtg attgtgcggg ccaccttgca
     1081 agcccggaaa cacggcgcgg tcgatgtcct tcgcgtactc gctcttgcag aggatcatgc
     1141 cgccgcgcgg tccgcgaaac gtcttgtgcg tggtgctcgt gatgatgtcc gcgatgccag
     1201 caggcgaagg atgcgcgccg ccgacgacca agccagagat gtgagcgatg tcagcgcaaa
     1261 gcttcgcgcc gacctcgtcc gcgatttccc ggaacttgtc gaactcgagg gtgcgcgcgt
     1321 acgcggaggt gccacaccag atgagcttcg gcttgtgctc gcgagcgagg gcgcgcacct
     1381 ccgcgaagtc gatgcggtga tcctgacggc tcaccccgta ggcgacgctc ttgaagtact
     1441 ttcccgtgat actcacgttc cagccgtggg tgaggtggcc gccggccggc agcgcgagcc
     1501 ccatcaccac gtcgtggggc tgacagaagg cgagatacac cgccaggttc gcggggctgc
     1561 cgctgtacgg ctggacgttc acgtggtcga cgccgaagag cgccttcacg cgggagcaag
     1621 cgagctcctc gacctgatcg atgatctgct gtccctcgta gtagcgcttg cccgcgtagc
     1681 cctcggaata cttgttcgtc aaaacggagc ccgacgcctc gagcacggcg cgcgagacgt
     1741 agttctcgct cgggatgagg cgcagcttca ccgtttcccg gacctcctct tctttaatga
     1801 ggcgcgcgat ttctgggtca cgggtcgaaa gcgggggatc agtgaggtgg ttggggctgg
     1861 gcatggcggc gcagtgggta ctcgcggcgc cccggccgcg ccagtgaact tcaacaggtt
     1921 gaaaataatg aacaattttc tattcaagca tcacggtaag cgctcgaacg agaagattgt
     1981 cactccctct cgacggcccc acccggggga ctaaactcgc gccggcgatg gccgagccta
     2041 gtaaagccaa gaactccccc gcgggggaga gctcccaggc ttcgcaggag cggcgcagta
     2101 cggcggtgcg agactccgtc tcgcccatag tcgtctctca cgaggcgaaa gccgcacctt
     2161 ctatcg
//
LOCUS       DASTKS010000667         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1668703, whole genome shotgun sequence.
ACCESSION   DASTKS010000667 DASTKS010000000
VERSION     DASTKS010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668703"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFQ35_13760"
     CDS             <1..193
                     /locus_tag="VFQ35_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9931760.1"
                     /translation="VLTGMGRDGERGARAIRDHGGVVLAESESSCVVYGMPRAVIEAG
                     LAQAEVPLDRMRSEIERWL"
     gene            431..1666
                     /gene="lysA"
                     /locus_tag="VFQ35_13765"
     CDS             431..1666
                     /gene="lysA"
                     /locus_tag="VFQ35_13765"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9931761.1"
                     /translation="MLPSVSGSLLAEIATRYGTPCWVYDASVIRRQIEQLRAFDVIRF
                     AQKANSNVHILRLMRSEGVQVDAVSLGELERALLAGYEVGAHPSPIVFTADLLDRPTL
                     KRVVELKVPVNAGSPQMLEQVGALSPGHPVWIRINPGFGHGHSKKTNTGGEHSKHGIW
                     HTHLADCLSLVDRHRLELEGLHMHIGSGVDYAHLGNVCDAMVSEVKRSGRDLAAISAG
                     GGLTIPYRSGDPVVDTAHYYELWDAARREIASFLGHPVKLEIEPGRFLVAQSGVLVSE
                     LRAQKPVGENLFTLVDAGFNDLMRPAMYGSHHRISLLSADGTPRAGTERPTVVAGPLC
                     ESGDVFTQAEGGEVLTRLLPPGEVGDWVVFHDAGAYGSSMSSNYNTRPLIPEVLVDGG
                     QVRLIRRRQTVDELLALER"
     gene            1808..2293
                     /locus_tag="VFQ35_13770"
     CDS             1808..2293
                     /locus_tag="VFQ35_13770"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9931762.1"
                     /translation="MNSDDLTLTLSLAASPEAVFAAINDPRAWWSGTIEGQTDALGAE
                     FTYAYKSMHRSRQRVSEWVPGKRVVWHVIDSQLTFVKDPSEWKGTDIVFDLVRQGDGT
                     QLTLTHRGLRPTCECFDACSSGWSSLATENLRSLVERRKTAQSDKSGSEPMPGVRSAS
                     R"
     gene            2297..3007
                     /locus_tag="VFQ35_13775"
     CDS             2297..3007
                     /locus_tag="VFQ35_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9931763.1"
                     /translation="MKDYLLLVLEDERAHASESPRATAALIEQRSAFIEELRRTGQLK
                     DSGRLRPSKEGYRVRRTSADVETESGPFGAERVALGAYYYVEAASLDAAAQLAESCPT
                     LASDELDVRPVMKGRLAADKDQKPGKIFACGVLGNAPNERAWVEVMDRIDAHKDSFPP
                     GAMLGGVRLDPPKTGKRVATRGGTRGVFDGPFLESKEVIGGLFFLRLASVDDVLRWAS
                     GVHFVEHGALEIRELWRS"
     gene            2995..4209
                     /locus_tag="VFQ35_13780"
     CDS             2995..4209
                     /locus_tag="VFQ35_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931764.1"
                     /translation="MAELSAFVDDVVRRERGRIVGALLRLGRSLDAAEEAFQEAVVAA
                     LQSWPQQTPANPGAWLMTAAKHSMSDARRRRAVADAKAPLLVEDDVDPGDIDTISDDH
                     LRLILTCCHPDLAVDSQIALTLKLVAGFSTEEIARAFVCSEATVSQRILRAKRAIESS
                     ELRDSTPDSADLEGRVGAALGVVYSLFNEGHTGRTGPLMRLDLQAEALRLGRLLCDLV
                     PGEPDAFALLAIMTFSAARAHTRVDEDGAPILLAEQDRGLWNRELLREGLVALARARS
                     LGGQGAYVLLASIAATHVTAPTWESTDWGTIVELYDTLARVSPSPIVELNRAIAVSMV
                     DGPSAGLALLGKLEPALASYHLFYATRAELLERLGRNALPDLQRALELATNDAERKLL
                     EKRLGSATRGTV"
     gene            4526..>5044
                     /locus_tag="VFQ35_13785"
     CDS             4526..>5044
                     /locus_tag="VFQ35_13785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9931765.1"
                     /translation="MKITLACGFLMLSVSATACSGGADSEGVLVEGAATEDSAILRPT
                     ATGGRDQIVMLYITVLTSSGFATRTCSGTYFAPRVVLTAAHCLENAYLDQVFVYYGDN
                     FTQDFPQLTQQGNLLVPPAPGSASVWAKADSFEKHPQWDPKLVHPDMGVVYLDRKLPF
                     DPLPLARFRLDST"
BASE COUNT          877 a         1645 c         1649 g          873 t
ORIGIN      
        1 ggttcttacc ggaatgggca gagacggcga acgcggcgcg cgcgccattc gagatcacgg
       61 cggagtcgtg ctggcggagt cggaatcgag ctgcgtcgtc tatgggatgc cccgggccgt
      121 catcgaagct gggctcgctc aggcagaagt cccgctcgat cgcatgcgct ccgaaatcga
      181 gcgctggttg tgaagggcgt gcagctgcgg cgccggcgca actagagtcg tcgtttttgg
      241 cccgcggaga ggatgctccc gcccgtccgc tcccggcggc agtcccgctt tccggcgagc
      301 aagtccccgc tcccgagagc cgccaagccc ggcaggcgac cggaatcgct ggtttccgag
      361 ggcgagcccg cgcgacatct gctatgcggt cgcacttgca tcggtttcag aagaggactc
      421 gttgagcacc gtgttgcctt ccgtttcagg ttccctactc gccgaaattg ctacccgcta
      481 cgggacaccg tgttgggtgt acgacgcgag cgtgattcgc aggcagatag agcagctgcg
      541 agcgttcgac gtcatccgct tcgcgcagaa ggcaaactcg aacgtccaca tcttgcgctt
      601 gatgcgttcg gagggcgtgc aagtggacgc cgtctcgctc ggggagctcg agcgagcact
      661 gttagcgggt tacgaggtgg gcgcgcaccc gtcaccgatc gtttttacgg ccgacttgct
      721 agaccgaccc accctcaagc gagtggtcga gctcaaggtg ccggtcaacg cgggctcgcc
      781 gcaaatgctc gagcaagtgg gcgcgctcag tcctggtcat cccgtttgga ttcgcatcaa
      841 cccgggtttt ggtcatggcc acagcaagaa aaccaacacc ggcggagagc acagcaagca
      901 cggcatctgg cacacgcacc tcgcggattg cctcagcttg gtggatcgcc atcggctcga
      961 gctcgaggga ctccacatgc acatcggttc tggggtggac tacgcccacc tcggcaacgt
     1021 gtgcgacgcc atggtctccg aggtcaagcg ctcgggccgc gacttggccg ccatttctgc
     1081 cggcggtggc ctgaccattc cttaccgttc gggcgatccg gtggtggata cggcgcatta
     1141 ctacgagtta tgggacgctg cgcgacgtga gatcgcgagt ttcttgggtc atcccgtcaa
     1201 actcgagatc gagcccgggc gcttcttggt ggcgcagtcg ggtgtgttgg tgagcgaact
     1261 gcgtgcgcaa aagccggttg gcgaaaacct gttcacgttg gtggacgcag ggttcaacga
     1321 cctcatgcgt ccagccatgt acggtagcca ccaccgcatc agcctgctca gcgccgacgg
     1381 aacgccgcgt gccggtaccg aacggcccac cgtggtagcc ggccccctgt gcgaatctgg
     1441 cgacgtgttc actcaggccg agggcggcga ggtgctgact cggctattac ctccgggtga
     1501 ggtgggcgac tgggtcgtgt tccacgatgc gggcgcttac gggtcgagca tgtcctccaa
     1561 ctacaacacc cgcccgctca tcccggaggt gctcgtagac ggaggccaag tccgcctcat
     1621 ccgtcggcgt cagaccgtcg acgagctgct cgcactcgaa cgctgattcg tcctcgcgtt
     1681 cgatgggagc tcctgggtcg aggaaggccc cccaaaaatt cttggagcca tgtcggatcc
     1741 gggtcgcgtg gaacgtcgcc tcggtgaagg cgcaagcaaa ggctcgcgcc gaaccaggag
     1801 aaacacgatg aattccgacg acttgaccct cacgctctcc ctcgccgcgt cccccgaagc
     1861 cgtgttcgcc gccatcaacg acccacgcgc ctggtggtcc ggcaccatcg aggggcagac
     1921 cgacgccctc ggcgccgagt tcacgtacgc gtacaagagc atgcaccgct cgcggcagcg
     1981 ggtcagcgag tgggttcccg gaaagcgcgt ggtttggcac gtcatcgata gtcagctgac
     2041 cttcgtgaag gacccgagcg agtggaaagg gacggacatc gtgttcgacc tggtccggca
     2101 gggcgacggt acgcagctca ctctcaccca ccgcgggctg cgccccacct gcgagtgctt
     2161 cgacgcgtgt tccagtggct ggagctccct cgcgaccgag aacctgcgta gcctcgtcga
     2221 acgccggaag accgcgcaat cggacaagtc gggctctgaa cccatgccgg gtgtacgctc
     2281 ggcgagccgg tagaccatga aagactatct gctgttggtg ctggaggacg agcgagcgca
     2341 cgcttccgaa tcgccgcgag ccacggcagc gctcatcgag cagcgctccg cgttcatcga
     2401 agagctgcgc cgcacggggc aactcaaaga cagcggtcgg cttcggccga gcaaagaggg
     2461 ctaccgagtt cgccgtacct ccgccgacgt cgaaaccgaa tcgggtccct ttggtgccga
     2521 gcgcgttgcc ctcggagctt actactatgt cgaggcggcg agcctcgacg cagcggcgca
     2581 gcttgcggaa tcttgtccaa cccttgcctc cgatgaactc gacgttcggc ccgtcatgaa
     2641 gggtcgcctc gccgccgaca aagatcagaa gccgggcaaa atcttcgcct gcggcgtgct
     2701 cggtaacgct cccaacgaac gtgcctgggt cgaagtgatg gatcggatcg atgcgcacaa
     2761 ggacagcttc ccgccgggcg caatgctcgg cggggtgcgc ctcgacccgc cgaagaccgg
     2821 caagcgtgtg gcgacacgcg gcgggacgcg cggcgtgttc gacggaccat tcctcgagag
     2881 caaagaggtg atcggcgggc tattcttcct gcgcctggcg agcgtcgacg acgtgctgcg
     2941 ctgggcgtcc ggggtccact tcgtcgagca cggcgcgctc gaaatccgcg agctttggcg
     3001 gagctgagcg ctttcgtcga cgacgtggtg cgacgcgaac gcgggcgcat cgtaggcgcg
     3061 ttgttgcgcc tcgggcggag cctcgacgcc gccgaagaag catttcaaga ggcggtggtc
     3121 gcggcgctgc aatcgtggcc ccagcaaacg cccgcgaacc cgggcgcgtg gctcatgacc
     3181 gctgcaaagc acagcatgag cgacgcgcgc cggcggcggg cggttgcgga cgcgaaggca
     3241 cccctgctcg tggaggacga cgtggacccg ggcgacatcg acacgatcag cgatgaccac
     3301 ctgcggctga tcctcacctg ctgccatcca gacctcgccg tcgacagcca gatcgcgctc
     3361 acgctgaagc tcgtcgcagg tttctcgacg gaggagatcg cgcgcgcgtt cgtctgctcg
     3421 gaagcgaccg tgtcgcagcg aattctgcga gcgaagcggg ccatcgagtc gagcgagctc
     3481 cgggattcaa cgcccgactc cgccgatctc gaagggcgtg tcggggcagc gctcggcgtc
     3541 gtctattcgc tcttcaacga gggccacacc ggacgtacgg ggccgctcat gcgcctcgac
     3601 ctccaagccg aggcgctgcg gctcgggcgg cttctgtgcg acctcgtgcc aggcgaaccg
     3661 gatgccttcg cgctcctcgc aatcatgacg ttcagcgctg ctcgcgctca cacgcgggta
     3721 gacgaagacg gagcgccgat cctgctcgcc gagcaagacc gcggcctctg gaatcgcgag
     3781 ctgctacgcg aagggttggt agcgctcgct cgagctcgct cgctcggtgg ccaaggcgcg
     3841 tacgtcttgc tggcttcgat agcggcgacc cacgtgaccg ctccgacgtg ggagagtacc
     3901 gactggggta cgatcgtcga gctctacgac acgctcgctc gagtctcacc ttcgcccatc
     3961 gtcgagctca accgcgccat cgcagtgtcg atggtcgacg gtccctcggc ggggctcgct
     4021 ttgctcggca aactcgagcc cgcgctcgct agctaccatc tattttacgc gacgcgcgcc
     4081 gagctcctcg agcggttggg gcgcaacgcc ctcccggatc ttcagcgagc cctcgaactc
     4141 gcgaccaacg acgccgagcg gaagctcctc gagaagcgcc tcggctccgc cacgcgggga
     4201 acagtgtaat ccgcacgagt tgtgcaacgt acgcgggggt gtgcacgcca cgcgaaccgt
     4261 ctaacgcgct caagttgtgc aattcgacac aagttgcgca gagggcgagc ggccggacgt
     4321 tcctgcaacg gccacgcgac gcacgcccgg tcgatgtgct gcacaacgag acgcgcgttc
     4381 gaggagcgcg cagcagccgc aggttttgag cgacgtgggc cctgaacacc gccgaagggg
     4441 aagacgcccc aggtggatgg ccctcggctt gcaatggcgt ccaaccgagc cgtcaggttt
     4501 cgaaacatcc ctgcaggaga tttacgtgaa gataaccctt gcctgtggtt tcttgatgct
     4561 ctcggtgtcc gcgaccgctt gctcgggcgg cgccgattcg gagggtgtgt tggtcgaggg
     4621 cgccgctacg gaagacagcg ccattctgcg gcccacggcg acgggcggcc gagaccagat
     4681 cgtcatgctc tacatcaccg tgctcacctc gagcggattc gcgacgcgga cctgcagcgg
     4741 cacctacttt gctccgcgcg tggtcctcac cgcggcgcac tgcttggaga atgcgtacct
     4801 cgatcaggtg ttcgtttact acggcgacaa cttcactcaa gatttccccc agctcacgca
     4861 gcaaggaaac ttgttggtgc cgcccgcgcc gggcagcgcg tccgtttggg cgaaggccga
     4921 ctccttcgag aaacacccgc agtgggatcc gaagctcgtt caccccgaca tgggagtcgt
     4981 gtacctcgat cgcaagctcc cgttcgatcc gctgccgttg gcgcggttcc ggctcgactc
     5041 cacg
//
LOCUS       DASTKS010000668         4109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1893119, whole genome shotgun sequence.
ACCESSION   DASTKS010000668 DASTKS010000000
VERSION     DASTKS010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893119"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1013)
                     /locus_tag="VFQ35_13790"
     CDS             complement(210..1013)
                     /locus_tag="VFQ35_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9931766.1"
                     /translation="MPKPDNMDDDRADTRERILEAARRLIETEGKDAATTRAVAAAAS
                     VQAPTIYRLFGDKFGLLDATAEYALAKYVGEKSAKPPHPDPIEDLRRGWDEHVAFSLS
                     HPALFALMSGDQRTEPSPAAKAGTEVLRRKIHRLAEHGLLRVDEKRAVLLMQAVGVGV
                     VKRLLEQPAHERDPGLSVFARDVVLDALVAKRGAVVTGAVRAAIALKAGLSEVDSLSN
                     GERLLLEELLERIAAPRAKPTSGTSGTSPARERATPEVTSPRPRRRRTR"
     gene            1093..1947
                     /locus_tag="VFQ35_13795"
     CDS             1093..1947
                     /locus_tag="VFQ35_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9931767.1"
                     /translation="MIVVTGATGKLGSAIVRSLLGRSLGSKVVASARSPEEASELSAL
                     GVEIRQGDFAKPETLKSAFAGAGQVLLVSSNAAAYGEDPVAQHRAAIQAAKAAGARRV
                     LYTSHVGASETSLFAPMLTHAATERMLAESGLAWTSLRNGFYASSALGFIGNGLKSGT
                     ISAPLDGKVAWTTHADLAEAAAAILASEGSFDGPTVLTGGEALDMDDLARIASEVLRR
                     EIRRVVVPDEDFEADLVVRHVPLPLVRITLGFYAASRNREFARVDGTLASLIGRKPQS
                     MRDVFGQL"
     gene            complement(2058..3080)
                     /locus_tag="VFQ35_13800"
     CDS             complement(2058..3080)
                     /locus_tag="VFQ35_13800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9931768.1"
                     /translation="MLSSAPAAAPSPRWVELSVVAPMYNEETNIQPFVDAVSSVLDTA
                     GIDYEIVLVDDGSRDGTWAAIGRAASGRSRVRGLSLSRNFGHQGALFAGLTQAQGRAV
                     VSMDGDLQHPPSAIPKLVEAWREGFQVVNTRRTDSADTSLFKRLTSRGFYWFFSRISG
                     VQMEAGASDFRLLDREALNALLSMGDADLFIRGLVSWLGFRTKTLDYQAQRRHSGVPK
                     FTLKKMLKFSSGAMLSFSVIPLRLGIGVGFVTSCFAFLEFGYILFAYFTGRTVTGWAS
                     MMAVMSLMFGVLFILLGIIGAYLGKIYEILKNRPRFVIGNRVGFEGSDSDRRRSRDRE
                     SATGFS"
     gene            3246..4019
                     /locus_tag="VFQ35_13805"
     CDS             3246..4019
                     /locus_tag="VFQ35_13805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9931769.1"
                     /translation="MSLYGKLSAEFYDLDKPEAPPDALEYYLQQAAHAAGPILEPMCG
                     TGRFLLPLLAAGFDVEGSDASAEMLALCRANAERLGLDARLAEQRLEELETSRKFGLV
                     FVPSGSFCLLTDTEVVKKCLQRIHAALAPRGRFIFEVERRVRQKPSELSGAWGGRWVS
                     RPDGAKLLFSWLSRYTAPSGIFSAVHRYELVADGRLIQQEFEDFELKLYELSEIRQLL
                     HAAGFPKIQMLEAYSQEPVNETSEAQDEEGIIVVAERGP"
BASE COUNT          715 a         1298 c         1382 g          714 t
ORIGIN      
        1 tggaatgcca agattcagct cctgaagcgc cctctgaaca ttctgctcac gatttggatg
       61 tcgagctcag cgtcgtgggc ggggccgatc ggtgctgatt cggcgccgac gagcgcagcg
      121 gtcgactgga ttcgcgttta cgactttcgg gggtgagtcg cgtaccctcg ggctctaagc
      181 gcacgtaggc gcccaacgac tccttgcgtt caacgtgtgc gacggcggcg tgggcggggc
      241 gaagtcactt cgggcgtcgc gcgctcgcga gcgggggagg tgcccgatgt cccagatgtc
      301 ggtttggccc tcggagcggc gatccgttcg agtagctctt ccagcagaag tcgctcgccg
      361 ttcgagagag agtcgacctc gctcaagccc gccttcagcg cgatcgcagc gcgcaccgcg
      421 cccgtcacga cggcgcctcg tttggccacc aaagcgtcca ggacgacgtc gcgggcaaac
      481 accgaaagac cagggtcgcg ctcgtgggct ggctgctcga gcaaacgttt gacgacgccg
      541 acgccaaccg cttgcatcag gagaaccgct cgcttctcgt cgacacggag caggccgtgc
      601 tccgccaatc gatggatttt tcggcggagc acttcggtac cggcttttgc cgcgggcgag
      661 ggctcagtgc gctgatcgcc gctcatcagc gcaaacagcg cggggtgtga gaggctgaag
      721 gcgacgtgct cgtcccagcc gcggcgcagg tcctctatcg gatcggggtg gggcggcttc
      781 gcgctcttct cgccgacata cttggcgagc gcatactctg cggtcgcgtc gaggagcccg
      841 aacttgtcgc cgaagaggcg atagatggtc ggcgcctgta ctgaggctgc ggccgcgacc
      901 gcccgcgtcg tcgccgcgtc cttcccttcg gtctcgatca ggcgtcgcgc cgcctcgagg
      961 attcgctcgc gggtatcggc gcggtcgtcg tccatgttgt ccggcttggg catatgactc
     1021 gaggcggggg ttgcgaaccg ttaacggcga tactatgaca ttgttatcgt cgttaacaag
     1081 gaggcgccac gcatgatcgt cgttacggga gctacaggaa agttgggcag cgcgatcgtg
     1141 cggtctttgc tcggccgctc gctcggctcc aaggtcgtcg cgagcgcgcg cagcccggag
     1201 gaggcgagtg agctctcggc gctaggcgtg gagatccgtc agggcgactt cgccaaaccc
     1261 gagacgctga agtcggcttt cgcgggggcg gggcaggtcc tgttggtctc gtccaacgcc
     1321 gccgcttacg gtgaggatcc ggtcgcacag catcgtgccg cgatccaagc cgcgaaggca
     1381 gccggggcgc ggagagtgct ctacacgagc cacgtgggcg cgagcgaaac gtcgctcttt
     1441 gcgcccatgc tcacccatgc cgcgaccgaa cggatgctcg ccgagagtgg tctcgcttgg
     1501 acttcgcttc ggaacggctt ctacgcatcg agcgccctcg gcttcatcgg caacggtctc
     1561 aagagcggca ccatctcggc gcctctcgac ggcaaggtgg cgtggactac tcacgccgat
     1621 ctcgccgagg cggcggcagc aattctcgcg agcgagggct cgttcgacgg ccccaccgtg
     1681 ctcaccgggg gcgaggcgct cgacatggac gacctcgcgc gcatcgcgag tgaagtgctg
     1741 agaagggaaa ttcgtcgcgt cgtggtgccg gacgaagact tcgaagcgga cctcgtggtt
     1801 cgccatgttc cgctgcctct cgtgcgcatc acgctcggct tttacgccgc gagccgcaac
     1861 cgcgagttcg cgcgcgtcga cggtacactc gcgagcctca tcgggcgcaa gccccagagc
     1921 atgcgcgacg tcttcggcca gctctgatgg ccaactaatt cgttccgagt tcttggagca
     1981 aagacgcagg cctacgtgct tgcgacgtga gacgaacgcc gagactgaag taggcggagg
     2041 agcgcagcgt agcccgctca gctgaagccg gtcgcgctct cgcggtcgcg gctgcgacgt
     2101 cggtcgctgt cgctgccctc gaagccgacg cggttgccga tgacgaagcg gggccggttc
     2161 ttcaaaattt cgtaaatctt accgaggtag gcgccgatga tgccgagcaa gatgaagagc
     2221 acaccgaaca tcaggctcat caccgccatc atcgaagccc agccggtgac ggtgcgcccg
     2281 gtgaaatagg cgaacaagat gtagccgaac tcgaggaagg caaagcagct cgtgacgaag
     2341 ccgacgccga tgccgagccg cagcggtatc accgagaacg acagcatcgc ccccgacgaa
     2401 aacttgagca tcttcttcag cgtgaacttc ggtacgccgg agtggcggcg ctgcgcttgg
     2461 tagtccagcg tcttcgtgcg gaagccgagc cagctgacga gaccccgaat gaaaagatcg
     2521 gcgtcgccca tgctgagcag ggcgttcagc gcctcgcgat ccaacaagcg aaaatccgat
     2581 gcgccggcct ccatctgcac gccggagatg cgcgagaaga accagtagaa gccgcgcgag
     2641 gtgaggcgct tgaagagcga cgtgtcggcg ctgtcggtgc gtcgggtgtt gacgacttgg
     2701 aagccttcgc gccaggcctc gacgagcttc gggatggcgc tcggtgggtg ttgcaagtcg
     2761 ccgtccatgc tgaccacggc ccgaccctgg gcctgcgtca gtccggcgaa gagcgcgcct
     2821 tggtgcccga agtttcgaga gaggctgagg ccgcggacgc gtgagcggcc cgatgccgcg
     2881 cgaccgatgg ccgcccaagt accatcgcga ctgccgtcgt cgacgaggac gatctcgtag
     2941 tcgatccccg ccgtgtccaa gaccgagctc accgcatcga cgaacggctg gatgttcgtc
     3001 tcctcgttgt acatcggggc gacgacggac agttccaccc agcgcgggga cggagcggcg
     3061 gcaggagcgc tggagagcat cgtcgcggat gctagcgcat ctgagaatta cgcaaggtgg
     3121 tctccgaacg cgcttgccgg cgccgtggcg gagggccgaa attgccggtc acgcgcgtca
     3181 ccgtgcccgg agagggctaa atttccggcg ttgcggcgca cggcacccgg gcgcaggctc
     3241 cgcgggtgtc cctctacggc aagctcagcg ctgaattcta cgacctggat aaacccgaag
     3301 ctccgccgga tgctctcgag tactacctgc agcaggccgc gcacgcagcg ggaccaatcc
     3361 tcgagcccat gtgcggcacg gggcgcttct tgttgccgct gctcgctgcc ggcttcgacg
     3421 tggagggcag cgatgcctcg gctgagatgc tcgccctttg ccgcgccaac gcggagcgcc
     3481 tcgggctcga cgcgcgcctg gccgagcagc gtttggagga gctcgagacg tcgcgaaaat
     3541 tcgggctcgt cttcgtcccg tccggctcct tctgcctgct gacggacacc gaagtggtga
     3601 aaaagtgcct ccagcgcatt catgccgcac tcgcgcctcg ggggcgcttc atcttcgagg
     3661 tcgagcgccg agttcgacag aagccgtcgg agctgagcgg ggcttggggt ggtcgctggg
     3721 tgtcgcggcc cgacggcgcg aagctcctct tcagttggct ttctcgctac acggcgccga
     3781 gcggcatctt cagcgcggtg catcggtacg agctcgtcgc cgacggtcga ttgatccagc
     3841 aagagttcga agacttcgag ctcaagcttt atgagctgtc ggagatccgt cagctgttgc
     3901 acgccgcggg cttccccaaa atccaaatgc tcgaggccta ctcgcaagaa ccggtgaacg
     3961 agacctccga agcgcaagac gaagagggca tcatcgtggt cgccgagcgc gggccctagc
     4021 ctagcagtgc gttgaaacgc cctgctagaa tgctgaccgg cactaccgtg cggctacgca
     4081 cccacgaaag cccatgaatg tgcggcttt
//
LOCUS       DASTKS010000669         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_107418, whole genome shotgun sequence.
ACCESSION   DASTKS010000669 DASTKS010000000
VERSION     DASTKS010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107418"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFQ35_13810"
     CDS             complement(<1..635)
                     /locus_tag="VFQ35_13810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET9931770.1"
                     /translation="MSRSRAPAPTAKLGLLAPHPVQLSTDPPPEEGKKRRRRSKASVI
                     EVRRIHRRDLNAVWDFLKLSFRDVNRATVEYQRPRTKQRFLETYEEEGVEQLLFLVNK
                     QIVAYAECTYEIMGKDTWLNPSYFERKDMRPLYVEELAVHPDYHGRGVGSFVLEQLQH
                     LARVRGLTHLVLEVAENNEHALAWYRKRGFRKLDAAIFLAQSIETEPELLPP"
     gene            complement(823..1329)
                     /locus_tag="VFQ35_13815"
     CDS             complement(823..1329)
                     /locus_tag="VFQ35_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HET9931771.1"
                     /translation="MLELDKHCTRSAAHRFDLRESATRISRRHRHRALAKESGVAPSR
                     PSASESPNPLTDASSERASSLVCAHCGAFVTSREQRIEIDGEHAHTFVNPAGMIFRIG
                     CFAVAPGVASVGEGSGDFTWFRGFVWQVALCRGCGEHLGWLFSSNAMRFFGLILDRLA
                     ESRAAPSD"
     gene            1808..>3019
                     /locus_tag="VFQ35_13820"
     CDS             1808..>3019
                     /locus_tag="VFQ35_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9931772.1"
                     /translation="MTRRFSRRVLLRGVGGACVAAPFLSSVMGRSVKAESVAATPKRL
                     ILMFTHYGCITTRFFPKLSHGKISAAELEPTTLKHLAPYADKLLLPRGIRSMNEWTAR
                     LERGQGNDMHIQACGSFFTCEPVRPNSDDPFSFQTNTRFQPRPIGASLDHVMAQQLSA
                     SKTPLLMRVGGTIDNALSAISYSAPETAFPGVTARQLFTNLTSLFQPGAPASPDSYRA
                     IRGKSIIDLVRDDLDALARADMSQADRLKLEAWKALLDDTGTVMSRAQCDPNAANTLL
                     LTEENMARATSNGGDDPLTRKVTDSLDAADLSSNLVVLAAICNLNPVIVLKYPGSFVF
                     RGLGLSSDSHSLSHRIGNAGLQGTCVADAIENIARMDDYNAQKFAHLVGKLDSIEEGD
                     ATVLDNSAVVWF"
BASE COUNT          546 a          949 c          961 g          563 t
ORIGIN      
        1 ggcggtaaga gttcgggctc tgtctcgatg ctttgcgcca agaaaatcgc ggcgtcgagt
       61 tttcgaaagc cacgcttgcg ataccaagcg agcgcatgct cgttgttctc ggcgacctcg
      121 agcacgaggt gagtgagccc tcgcacgcgc gctaggtgct gcagctgctc gagcacgaaa
      181 gagccaacgc cgcgcccgtg gtagtccggg tgcacggcga gctcttccac gtagaggggg
      241 cgcatgtctt tgcgctcgaa gtagctcggg ttgagccacg tgtcctttcc catgatctcg
      301 tacgtacact cggcgtacgc gacgatctgc ttgttcacga gaaagagcag ctgctcgacg
      361 ccttcttctt cgtaagtctc caagaagcgt tgcttcgtgc gcggtcgttg atactccacc
      421 gtcgcccggt tcacgtcgcg aaaagagagc ttcagaaagt cccacactgc gttcagatca
      481 cggcggtgga tgcgtcgcac ctcgatcacg ctcgccttcg accgccgtcg tctcttcttt
      541 ccctcttcgg gtggcggatc cgtagagagc tggacggggt gcggggcgag caagccgagc
      601 ttggccgtgg gggcaggggc gcgcgagcgg gacacgagac gtccgatggt agcaaagggc
      661 gcgcggcgcc attcaagagc atcgtccgcg tggtcggcct gctgaccgat gtcggcggtt
      721 ttggcgcaga agtcccgatt gcagaggggc ttgccgcgcc ctggcggtcg gcgaccaggc
      781 ccgatgtttg gtggcgggcc gtcgcccgct tcgagtttcg actcagtccg agggggcggc
      841 gcgcgattcg gcgagtcggt cgaggatcag tccgaagaag cgcatcgcgt tcgaactaaa
      901 gagccacccg aggtgctcac cacagccacg acagagcgcg acctgccaca cgaagccgcg
      961 aaaccaagtg aagtcgccgc tcccttcgcc cacactcgcg accccgggcg ccactgcaaa
     1021 gcacccgatg cgaaagatca tccccgccgg gttcacgaag gtgtgcgcgt gctctccatc
     1081 gatctcgatg cgctgctcgc gcgaagtgac gaacgcgccg cagtgagcgc atacgagact
     1141 cgatgcacgt tcggagctcg cgtcggttag cgggtttggc gactcgctag ccgatgggcg
     1201 cgacggcgct acgcccgatt ctttggcgag tgcacgatga cggtgacgcc gcgaaatgcg
     1261 ggtcgctgac tctcgcaggt cgaatcggtg agcagcgctc ctcgtgcagt gtttgtcgag
     1321 ttccagcatc gtgaattgcg tctccgtaat gtggcacaat ccacttgctg agtacgcccc
     1381 ccgcagtcgg ccttcgccgt cgcagcccgc cgactttcag tgaccgacgg cggttgggaa
     1441 ggcagagctc gaacgcactg ccggcctttc gaatgtcgct cagcggtgca attttgaaca
     1501 agcatggccc atgcgaggta tgaaattgca ggagaggaaa ccggcgggtt cggcagtttt
     1561 tgctcgccaa aagcgcgctc gtggtggttc gagggcgcgg cgcagcgtta ccgaaatacg
     1621 agcatgcgtc ggtgggcata catgtctgtt tggcggcgct cacacaattc cgcgcgggtt
     1681 tcgctcgcgg ctttcgagaa cgtggcggcg ggctcgaggc cggagtctct agtttcggcg
     1741 tcgggagcca gggctcactc gatgattcga ttcgcgcgcc gcaagcgagc gaaggaaagg
     1801 tcgaccaatg acgcggcggt tctcccgcag ggtgctgctc aggggcgttg ggggggcttg
     1861 cgtcgcggcg ccgttcctca gctcggtcat gggtcgctcg gtaaaggccg agtccgtcgc
     1921 ggctacgccc aagcgactca tcctgatgtt cacgcactac gggtgcatca cgacgcgctt
     1981 cttccccaag ctctctcacg gcaaaataag cgccgccgaa ctcgagccga ctacgctgaa
     2041 gcacctcgcg ccgtacgcgg acaaactgct tttgccgcgc ggcatccggt ccatgaacga
     2101 gtggacggcg cgcctcgagc ggggccaggg caacgacatg cacattcagg cttgtggttc
     2161 ctttttcacg tgcgagccag tgcggccgaa cagcgacgac cccttctcct tccagacgaa
     2221 cacgcgattt cagccccgcc ccattggtgc gtctctggat cacgtcatgg cgcagcaact
     2281 cagcgccagc aagaccccat tgttgatgcg agtgggtggc accatcgaca atgcgctctc
     2341 ggcgatctcg tactcggcgc cggagaccgc gttccctggc gtcacggcga ggcaactttt
     2401 cacgaatctc acgtcgctct ttcaaccggg agcgccggcg tccccggaca gctaccgggc
     2461 gattcgcggt aagagcatca tcgacctcgt tcgcgacgac ctggacgcgc tcgcgcgcgc
     2521 ggacatgagc caagccgatc gactcaagct cgaggcatgg aaagccttgc tcgacgacac
     2581 cggcacggtc atgtcgcgcg cccaatgcga tccgaacgcg gcgaatacgc tgctgctgac
     2641 ggaggagaac atggctcgcg cgacctccaa tggaggcgac gacccgctca cgcgcaaggt
     2701 caccgactcg ctggacgcgg ccgacctctc ttcgaacctg gtcgtgctgg cggccatctg
     2761 caacctgaac ccagtcatcg tcctcaaata cccaggtagc ttcgtgtttc gcgggcttgg
     2821 gctctcatcc gactctcact cgctttctca ccgcatcggg aacgcaggcc tgcaggggac
     2881 gtgcgtggca gacgcgatcg agaacatcgc gcgaatggac gactacaacg cgcagaagtt
     2941 cgctcatctc gttggaaaac tcgacagcat cgaagagggc gacgccaccg tgctcgacaa
     3001 ctcggctgtg gtttggttt
//
LOCUS       DASTKS010000670         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1447057, whole genome shotgun sequence.
ACCESSION   DASTKS010000670 DASTKS010000000
VERSION     DASTKS010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447057"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ35_13825"
     CDS             complement(<1..258)
                     /locus_tag="VFQ35_13825"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET9931773.1"
                     /translation="MPEEALLERLLRFGRALVVGSGATLTDFSIFTTCVRAFGVAPTV
                     ARVPALLAGACFQFFGNRSYTFRARAGKLSRQASLFVVAEAI"
     gene            complement(321..1349)
                     /locus_tag="VFQ35_13830"
     CDS             complement(321..1349)
                     /locus_tag="VFQ35_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9931774.1"
                     /translation="MPEPDPPISERQRLSLNALPPQLASRAHAWLGWSHQGRISLDVL
                     DGALEGDVLRAASAMAFDLFLAAIPLLALAGWVFAKFVSSGSAAQAMSLLLDTTPAAV
                     RSIAEEQLGQFQPSAFTPLALIGSVWLSSSAAATCMALLEARGHAEVRAWWSRRLLAM
                     ASVLIGIVVFGAGSALSLWIVGGPVAILEAFAGARVAAWTRYALGLSLLYLLVVVLLA
                     MFMRVAVRRPSIHQRRVFPGAVLGAVLCVVASAAFTFYASRIARFAFYYGGLAAVAVT
                     LVWLWLMCLFILVGAELNLVLEGGGSAGRALEERERGPLLADLVSQTEAVRDRETVKT
                     SSSGPPRA"
     gene            1394..1813
                     /locus_tag="VFQ35_13835"
     CDS             1394..1813
                     /locus_tag="VFQ35_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9931775.1"
                     /translation="MNVSQQTLEISTRGRGFSDITREVAAVVEKSGIRRGICSVFVQH
                     TSASLVIQENADPRVLKDLAAFMADLVPESRDWEHDDEGPDDMPAHARSALTRTSETL
                     PIVNSRLALGTWQALYLWEHRARPHRRSLVVTVMGDG"
     gene            1948..2520
                     /locus_tag="VFQ35_13840"
     CDS             1948..2520
                     /locus_tag="VFQ35_13840"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HET9931776.1"
                     /translation="MFDLRTATPPAWLDAVFADFDSFLIDHAACERKASATGMSFVVR
                     YPDKQSLIEPMIEFAREELEHFHIVYKLVAERGLTLVADYKDPYVNALRAECRHGAEL
                     LDRLIVSGVVEARGCERLFMVAGALEERDPRLSNIYLDLARAESRHHGLFFRLARQLF
                     DEPQVVERAGELLDFEARLVETLPHRAAVH"
     gene            2532..>3600
                     /locus_tag="VFQ35_13845"
     CDS             2532..>3600
                     /locus_tag="VFQ35_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931777.1"
                     /translation="MAAGAAFAKYLRRYAEAEARQVPRLALPHHAALVVPAFAESEVM
                     LDGYRDAARAAPGPVVVILVVNAPASASDEERAANAALLRSLRARASYDDGQFVFVAE
                     PEFSLLVVDRASPGREIEKKLGVGHARKIGADVALALFARGELESRFVFSTDADACLP
                     LDYFVRPRADEVAGALLFPFEHVEGSDPRVFEMTLLYELSLRYHVLGLAFAGSPYAHH
                     SVGSALAASYEGYAAVRGFPKRAAGEDFYLLDKVGKVLPIRRLAGAPVRILARRSLRV
                     PFGTGPRVERLLSEPSAFVDHPATYQALAALLAALDAFAQSRDDGSFARAFTDLPRDL
                     ATAADAAARELGILEAARGAIA"
BASE COUNT          611 a         1227 c         1174 g          588 t
ORIGIN      
        1 tatcgcttcg gccaccacga acaggctcgc ttgccgcgac agctttcccg ccctggcgcg
       61 gaacgtgtag cttcgatttc cgaagaattg aaagcaagcg ccggcgagca gcgcgggcac
      121 gcgcgcgacg gtcggtgcga cgccgaacgc ccgcacgcag gtcgtgaaga tggagaagtc
      181 ggtcagggtc gcgccgctac cgacgacgag cgcgcgcccg aaccggagca ggcgctcgag
      241 cagggcctct tcaggcatgc gcgtatgaaa ccgcaaaacc agcgaaccga aacggggtat
      301 tcgcgctcgt cgcgaggatg tcacgcgcgc ggcgggccgc tcgaggaggt cttgacggtc
      361 tcgcggtctc gaaccgcttc cgtttggctg acgagatccg cgagcagcgg tccgcgttcg
      421 cgctcttcca aggctcgacc cgcggaaccg ccgccttcga gcacgaggtt cagctccgcg
      481 cccacgagga tgaacaggca catcagccag agccagacga gcgtgaccgc gacggcggcg
      541 agccctccgt agtagaaagc gaaacgggcg atgcggctcg cgtagaacgt gaacgcggcc
      601 gacgcgacca cacacagcac ggcgccgagc accgcgcccg ggaagacgcg acgctgatgg
      661 atgctcgggc ggcgcaccgc gacgcgcatg aacatcgcga gcaacacgac gaccaagaga
      721 tacaaaagcg agagcccgag cgcgtagcgc gtccacgctg ccactcgcgc gcccgcgaac
      781 gcttctagaa tcgcgactgg cccgcccacg atccagagcg agagcgcgct cccagcgccg
      841 aacaccacga tgccgatcag cacggacgcc atcgccagca aacgccggct ccaccaagcg
      901 cgcacctcgg cgtgaccgcg cgcctcgagc agcgccatgc acgtcgccgc cgccgaggag
      961 ctcagccaaa ccgaaccaat cagcgcgagg ggtgtgaagg cagagggctg aaactgcccg
     1021 agctgttctt ccgcgatgct gcgcaccgcg gcgggagtgg tgtcgagcaa aaggctcatt
     1081 gcctgcgcag cgctgcccga gctcacgaac ttcgcgaaca cccagcctgc cagcgcgagc
     1141 agcggaatcg ccgcgaggaa caggtcgaac gccatcgcgc tcgccgcgcg cagcacgtcg
     1201 ccttcgagcg cgccgtccaa cacgtcgagc gagatcctcc cttggtgact ccagccgagc
     1261 caagcgtgcg ctcgggacgc gagctgtggc ggcagcgcgt tgagagacaa ccgctgacgc
     1321 tccgagatgg gagggtcggg ttccggcatc aaattcctgg cagagtaccc cgaatcatcg
     1381 catatcttcg ccggtgaacg tctcccaaca aacgctcgaa atcagcacgc gcggccgcgg
     1441 cttctccgac attacccgcg aggtagccgc cgtcgtcgag aagagcggca ttcggcgcgg
     1501 gatttgctcg gtgttcgtcc aacacacgtc cgcgagcctc gtcattcaag agaacgcaga
     1561 cccccgcgtt ttgaaggatc tggctgcttt catggcggat ctcgttccgg agagccgtga
     1621 ttgggagcac gacgacgagg ggcctgacga catgcccgcc cacgcgcgct ccgccctgac
     1681 ccggacgagt gagacgcttc cgatcgtgaa ctctcgcctc gcgctcggca catggcaggc
     1741 actttacctt tgggaacatc gcgcgcgccc gcatcgccgt agcctcgtgg tcacggtcat
     1801 gggagacggc tgagctcaga ccgcagattc gcggtccacg gcgagcgagg actgcaaatc
     1861 cagcggccct ttggggcgat ctgttgccag gtgtttgtct tgttgcatcc ccccaagcga
     1921 gcttgggtgt aaagtccgcc ccgacccatg ttcgacctcc gtaccgcgac gccgcccgct
     1981 tggctcgacg ccgtcttcgc cgacttcgac tcgtttttga tcgatcacgc cgcgtgtgag
     2041 cgcaaagcct ccgcgaccgg catgtcgttc gtggtgcgct acccggacaa gcagagcctg
     2101 atcgagccga tgatcgagtt tgcgcgcgag gagctcgagc actttcacat cgtctacaag
     2161 ctcgtcgcgg aacgcggcct cacgctcgtc gccgactaca aggatccgta cgtgaacgcc
     2221 ctgcgcgccg agtgccgaca cggcgccgag ctgctcgatc gtttgatcgt gtcgggcgtg
     2281 gtcgaggcac gcggctgcga gcggttgttc atggtcgcgg gagcactcga ggagcgcgac
     2341 ccgaggctgt cgaacatcta cctcgacctg gcccgcgccg agtcgcggca ccatgggctg
     2401 ttcttccgct tggcgcgcca actcttcgac gaaccacagg tggtggagcg tgcgggcgag
     2461 ctgctcgact tcgaagcgcg cctggtcgaa actctgccgc atcgcgccgc cgtgcactga
     2521 ggatcccgcg aatggctgcg ggggcggcgt tcgccaagta tcttcgtcgt tacgccgaag
     2581 ccgaggcgag gcaggtgccg cgtttggcgc tgccccatca tgccgctctg gtcgttcccg
     2641 cgttcgccga gtcggaggtc atgctcgacg ggtatcgcga tgccgcccgc gccgcacccg
     2701 ggcccgtagt cgtgatcttg gtggtgaacg cgcctgcctc ggcgtcggac gaggagcgcg
     2761 cagcgaacgc ggcactcctc cgtagccttc gcgctcgagc aagttacgac gacggtcagt
     2821 tcgtgttcgt ggcggagccg gagttttcgc tgctcgtcgt cgatcgcgcg agccccggcc
     2881 gcgaaatcga gaagaagctc ggcgtcggtc acgcacgcaa gatcggcgcc gacgttgcgc
     2941 tcgcgctgtt tgctcgcggt gagctcgagt cgcgcttcgt gttctcgacc gacgccgacg
     3001 catgcttgcc gctggactac ttcgttcgac cgcgcgccga tgaagtcgct ggcgctctgc
     3061 tgtttccctt cgagcacgtg gaaggctccg atccccgcgt tttcgagatg acgctgctct
     3121 acgagctttc gctgcgatac cacgtgctcg ggctcgcttt cgctggttcc ccgtacgctc
     3181 atcacagcgt cggcagcgcc ctcgccgcgt cgtacgaggg gtacgcggcg gtgcgcggct
     3241 ttccgaagcg cgctgccggc gaagactttt acctgctcga taaggtcggt aaggtgctgc
     3301 cgattcgtcg tctcgcgggc gctcccgtgc gcattctagc gcggcgctcg ttgcgggttc
     3361 cgttcggtac cgggccgcgc gtcgagcgct tgctcagcga gccgagcgca ttcgtcgatc
     3421 accccgcgac gtaccaagcg ctcgcggcgc tgctagccgc gctggacgca ttcgcacaaa
     3481 gtcgcgacga tggctccttc gcgcgcgcgt tcacggacct cccgcgagat ctagcgacag
     3541 cggccgacgc ggcggctcgc gagctcggca tactcgaagc agcgcgtggc gcgatcgccg
//
LOCUS       DASTKS010000671         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_553855, whole genome shotgun sequence.
ACCESSION   DASTKS010000671 DASTKS010000000
VERSION     DASTKS010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553855"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFQ35_13850"
     CDS             complement(<1..308)
                     /locus_tag="VFQ35_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931778.1"
                     /translation="MARFWSLMVVAVTLLAGRSARAELPPPGSPDERPLIPTAPPPPE
                     YPSAAARGRLALAGGALFAGWYAAAVAQSYGWKEAPAHERLLIPVAGPWMTVAHAGCS
                     "
     gene            421..1869
                     /gene="dacB"
                     /locus_tag="VFQ35_13855"
     CDS             421..1869
                     /gene="dacB"
                     /locus_tag="VFQ35_13855"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET9931779.1"
                     /translation="MIGARPSLLGRRGFTRGALGIAALLVQAPVRAASDALRMACSSA
                     LTELAGAAARNRFRLSACVAELETGEVLGDASAHVPQNPASNQKLLTMAVALDRLGPN
                     FRFATSLHGRALGVEALSELVLRGNGDPELSAATLEQLVRSLAEQGVRRVDGDLFVDQ
                     SAFDSLWDPPGYEQHPEEWAGYRAPVSAVSLAGNTVTLHVLGSESGPARAWLSPPGIA
                     SITGEILSGRGLPQNVRYSVRPRGTEFDVVVGGSVPTEKGELTFARRIENPELAPGRV
                     LRALLAEHGITVAGQVRSGGAEIEAERVASPSRSLAEIIHALGKRSDNFVAEMLLKAV
                     ASSDKAGPGSSARGARALEEHLTRLGALDAGSRLGNGSGLYDANRISAYALTRTLVSA
                     YNDPRVAPEFVAALSIGGLDGTLSQRFKAHRTERSVRAKTGTLADAIGLSGYLLTPRV
                     RVAFSVLLNGVNGKQQEARARIDSAVENILRA"
     gene            complement(2269..>2481)
                     /locus_tag="VFQ35_13860"
     CDS             complement(2269..>2481)
                     /locus_tag="VFQ35_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET9931780.1"
                     /translation="QGNLVVAMIIATIKAGLVVAFFMHLVWDKKLHLLLFLTSVLFLI
                     LFLSLSVNDRGEYQHDIDAFQNSAAK"
BASE COUNT          439 a          833 c          818 g          391 t
ORIGIN      
        1 ctcgaacaac cggcgtgcgc gaccgtcatc cacggaccgg caaccggaat gagtagccgc
       61 tcgtgcgcgg gcgcctcttt ccatccgtag gattgcgcaa ccgcggccgc gtaccacccc
      121 gcgaacaggg cgccaccagc caaggcaagg cgaccgcgcg cggcagccga cgggtactct
      181 ggaggaggcg gtgcggtggg gatgagcggg cgctcatcgg gagagcccgg cggtggcaac
      241 tccgcgcgcg cggagcgacc cgcgagcaac gtcacggcga cgaccatcaa cgaccagaat
      301 cgagccaccg aggcctcttt gcccgagttt gccctgaacg caagcacaat gcgccccggc
      361 gctcggttcg agcagctatc accacgccgt ttgttcggcg agctcgctta agatacgaaa
      421 atgatcggtg ctcgaccgtc tctgctaggc cgccgcggat tcactcgagg cgctctcggg
      481 atcgcggcgt tgctggtgca agcgccggtg cgtgccgcaa gcgatgcgct gcgcatggcg
      541 tgcagctcgg cgctcacgga gctcgcggga gcggcggccc gcaatcggtt tcggctcagc
      601 gcgtgcgtcg cggagctcga gacgggcgaa gtgttgggcg acgcttccgc gcacgtcccg
      661 caaaatcccg cttcgaacca aaagcttttg acgatggccg tcgcgctcga tcgcctcggc
      721 cccaatttcc gctttgccac gtctcttcac gggcgcgcgc tcggagtgga agcgctctcc
      781 gagctcgtgc tgcgtggcaa cggcgacccg gagctctccg ccgcaacgct cgaacagctg
      841 gtccgctcac tcgcggagca aggcgtgcgc cgcgtagacg gcgatctttt cgtagatcag
      901 agcgctttcg attcactctg ggatccgccc ggttacgaac agcaccctga ggagtgggca
      961 gggtaccgtg cgcccgtcag cgcggtgagc ctcgccggga atacggtgac gctgcacgta
     1021 ctgggctccg agtcggggcc ggcgcgcgca tggctctctc cgcccggcat cgcgagcatc
     1081 acgggagaga tcctctccgg gcgtgggctc ccgcaaaatg tgcgttacag cgttcgcccc
     1141 agagggaccg aattcgatgt ggtggtcggt gggagcgttc cgaccgagaa gggcgaactg
     1201 accttcgcgc gacgcatcga gaacccggag cttgcgccgg gcagagtgtt gcgcgcgctg
     1261 ctcgcggagc acggcatcac ggtcgcggga caggtccgct ccggaggcgc cgaaatagag
     1321 gccgaacggg tggcttcgcc gtcgcgctca ctcgccgaga tcatccatgc gctcggcaag
     1381 cgcagcgaca acttcgtcgc ggaaatgctg ttgaaagccg tcgcctcgag cgacaaagcc
     1441 ggccccggga gctctgcgcg cggcgctcgc gctctcgaag agcacctcac ccgtctcggc
     1501 gcgctcgacg caggctcgcg tctcggcaac ggctccggcc tctacgacgc gaaccggatc
     1561 agcgcctatg cgctcacgcg gacgctcgtg agcgcctaca acgatccgcg cgtcgccccc
     1621 gagttcgttg ccgcgctgtc gatcggtgga ctggacggca cgctgtccca acgcttcaag
     1681 gcgcaccgca ccgagcgcag tgtgcgcgcc aagacaggca cgctcgccga cgcgatcggg
     1741 ctatccggct acctgctcac gccgcgcgtt cgcgtcgcct tctcggtgct cttgaacggt
     1801 gtgaacggca aacaacaaga agcgcgcgcc cgcatcgaca gcgccgtcga gaacattctg
     1861 cgcgcctgag ctttttcaac gccaaggctc cgaggtgagc caaggccgcc gaattttttg
     1921 tggggagcga tgcgacggag acgtttcgct cgagcgctgt gaaaggcggg cgcgaagtga
     1981 gaaggttcga ttcgcgacgg agcgaacccg agcgaaacgt tccggtggtc ggcgctcacc
     2041 cccaaatcct cagcgctctt ggaccgactc tcggcgcctt ggcgttcaac caagcgccca
     2101 gattcgtcgg ccttcaacca ggcgccggat tcatcggcct tcaaccaggc gcccagagcc
     2161 gtcggccttc aaccaggcgc ccagagccgt ccgccttcaa ccaggcgccc agagccgccg
     2221 gccttcaacc aagcgcccag agccgttcgg gcgagcgagc acgagagctt acttggcggc
     2281 ggagttttgg aacgcgtcga tgtcgtgctg gtactcgccg cggtcgttca cgctgagcga
     2341 caagaacagg atcaggaaga gcacagaggt gaggaacagg agcaagtgca gcttcttgtc
     2401 ccagaccaag tgcatgaaga acgcgacgac cagcccggcc ttgatggtcg cgataatcat
     2461 tgcgaccacg aggttgccct g
//
LOCUS       DASTKS010000672         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1447584, whole genome shotgun sequence.
ACCESSION   DASTKS010000672 DASTKS010000000
VERSION     DASTKS010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447584"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1567)
                     /locus_tag="VFQ35_13865"
     CDS             complement(41..1567)
                     /locus_tag="VFQ35_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931781.1"
                     /translation="MVTALLLTAVLAACSAGESSESSDAGAAGVAKGGRSAASGGATS
                     SGGSAASGSANGGAASGGVQNTGAGGASNPSSGGKASASGGAAASGGAAGSGGAKASS
                     GGSSGGNASQMGGRAGTGGNTGGAGAASGGKASVGGAGSGGASGGGTSMGGAASGGAG
                     STQWAAGNPNGSCKSSLPSRAQPADTSSPTTVVGTGTAASCTFAKLQTAVTQGGVITF
                     DCGASPLTIPVTATLKPPTNKNTVIDGGRKITLDGGKAVQLINFTSPDFRANDHGLTL
                     QHIALVNGKQTPTEAIPPADPPCSQGFNDGEGGAVFMRDGYLNVIDSIFTGNQAAPLG
                     PDTGGGAIYVLGSKAGVVIAGSTFTNNAASNAGAVGGLFAELQIYNSYFSNNTALGHD
                     ANNDDASKCDAMNNGQHEIGSGGNGGAIYSDGNSVNVTLCGDAIVNNAAGTKAFGGGL
                     FFTSNDMGGTLSIADTTMSGNTGGHWTNVAGGSMNNAGTAVGTNCKSLSITNSTLQGL
                     "
     gene            complement(1786..>2491)
                     /locus_tag="VFQ35_13870"
     CDS             complement(1786..>2491)
                     /locus_tag="VFQ35_13870"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9931782.1"
                     /translation="TQRRLANSLPGEPPRYVEPVVPVIFPESAEVPETQLHFEVQTIL
                     YQLLFDHLGPDITVGSNQFVYFNAAEPKQCLAPDVYVRLAPRGAPIRSWKVWERGAPE
                     IAVEIVSDDDREADVWELKLRRYRQLGVSELVRFDPEASGTAALRIWDRVDGALLERE
                     LTGASAPSVVLNFDWVLAPADGFELALRIGVGEDGRTLLLTRAEARKVEAEARQAAEA
                     RVRELEAELARRSDPK"
BASE COUNT          368 a          874 c          781 g          468 t
ORIGIN      
        1 atcggcgcca aacgtacgag tcagtgctct cgccgcgacc tcaaagtccc tgcaacgtcg
       61 agttcgttat cgagaggctc ttgcagttcg tgccgacggc ggtgcccgcg ttgttcatgc
      121 tcccgcccgc gacgttcgtc caatgccctc cggtgttgcc ggacatggtc gtgtccgcaa
      181 tgctgagcgt cccgcccata tcgttgctcg tgaagaacaa cccgccgccg aacgccttcg
      241 tgccggccgc gttgttgacg atcgcgtcgc cacacaaggt gacgttcact gaattgccgt
      301 cgctgtaaat cgcgccgccg ttgccccccg aaccgatctc gtgctggccg ttgttcatgg
      361 cgtcgcactt gctcgcgtca tcgttgttcg cgtcgtggcc gagggcggta ttgttcgaga
      421 agtagctatt atagatctgg agctcggcaa acaggccgcc gaccgcgccg gcgttgctcg
      481 cggcgttgtt cgtgaaggtg ctccccgcga tgacgacgcc cgccttgctg ccgagcacgt
      541 agatcgcacc gccgcccgtg tcaggcccca acggggcggc ttggttgccg gtgaagatcg
      601 aatcgatcac gttcaagtag ccgtcgcgca tgaacaccgc gccgccttcg ccatcgttga
      661 agccctgcga gcacggcgga tccgccgggg ggatggcttc ggtcggggtt tgcttgccgt
      721 tcacgagagc gatgtgctgc agcgtgaggc cgtggtcgtt cgcgcggaag tctgggctcg
      781 tgaagttgat gagctgcacc gctttaccac cgtccagcgt gatctttcga ccaccatcga
      841 tcaccgtgtt cttgttggtc ggcggcttga gcgtcgccgt cactggaatc gtgagggggc
      901 tcgcaccgca atcgaatgtg atgacgccgc cctgagtcac cgccgtctgc aacttcgcga
      961 acgtgcagct cgcggcggtg ccggtgccga cgactgtcgt cgggctcgag gtgtctgcgg
     1021 gctgcgctcg cgagggtaga ctcgacttgc acgagccatt cggatttccg gcggcccatt
     1081 gcgtggaccc agctcccccg gatgccgcgc cccccatcga cgttccacct ccgctcgcgc
     1141 cacccgaacc ggcgcctccg acgctcgcct taccgccgct cgccgcgcca gctcctccgg
     1201 tgttaccccc ggtgcctgcg cgcccaccca tttggctcgc gttgccgcct gaagacccac
     1261 cgctagatgc tttcgctccc ccgctcccag cggcaccgcc gctcgcagcg gcgccccccg
     1321 aggcgctggc cttgccacct gagctcgggt tgctcgctcc tcctgctccg gtgttctgta
     1381 ctccgccgct cgcagcgccc ccgttggcac ttccgctcgc cgccgagcca ccgctggacg
     1441 tggctccgcc gctcgcagcg ctcctcccgc cttttgctac gcccgccgcg cctgcgtcgg
     1501 agctttctga cgactcccct gcgctgcagg cggcgagcac cgccgtgagt agaagagccg
     1561 tgaccaaccg agggagagct agagttgcca agctccagta cgacccccga cgcgggctat
     1621 tcggtccaag cgaggccgcg atgccgcacc gatgtcggga ttcgagcgac agtggctacg
     1681 ggctcgcctc cgcggccgct cgctttcgcg gcgtctcact ttcggatgcc tgcgacagcg
     1741 gccacggact cgcttccgcg gccgttcgct gctcgaagcg gcgtctcact tcgggtccga
     1801 gcgccgcgcg agctccgcct cgagttcgcg gactcgagcc tcagcggctt gccgcgcttc
     1861 ggcttcgacc tttcgcgctt cggcgcgtgt cagaagcaga gttcggccgt cctcaccgac
     1921 gccgattcgc aacgcgagct cgaagccgtc agctggcgcc agaacccaat cgaaattgag
     1981 caccaccgag ggggcgctcg caccggtcag ctcgcgctcg agcagtgcgc cgtccacacg
     2041 atcccagatg cgcagcgccg ctgtaccgga cgcttcggga tcgaagcgga cgagctcgga
     2101 aacgcccagc tgcctgtagc ggcgaagctt gagctcccag acgtccgcct cgcgatcgtc
     2161 atcgctcacg atttcgacgg cgatttctgg tgcgccgcgc tcccagacct tccacgagcg
     2221 aataggcgca ccgcgcggcg cgagacgaac gtagacgtcg ggagcgaggc actgcttggg
     2281 ctccgcggcg ttgaaataga cgaactggtt tgaccccact gtgatgtcag ggcccaggtg
     2341 gtcgaacaag agctgataca agatggtctg tacctcgaag tgcagctgtg tttcaggcac
     2401 ttcggcgctc tctggaaata tcaccgggac gaccggctcg acgtaacgag gcggctcgcc
     2461 ggggagagag ttcgcgaggc gacgctgcgt g
//
LOCUS       DASTKS010000673         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_999425, whole genome shotgun sequence.
ACCESSION   DASTKS010000673 DASTKS010000000
VERSION     DASTKS010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999425"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VFQ35_13875"
     CDS             <1..421
                     /locus_tag="VFQ35_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931783.1"
                     /translation="SKNKPNKVDCAIAKVLPNAKVSATPMRKVGRLASGEVIEATPGM
                     KVHKVGRTTSYTTGIVQDVNADVTVAYEVGAITFQDQIVIIGDAGSFSDSGDSGSMIV
                     DRATRRPTALLFAGSASHTIANHMQDVLDALAVEIVR"
     gene            843..>3043
                     /locus_tag="VFQ35_13880"
     CDS             843..>3043
                     /locus_tag="VFQ35_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9931784.1"
                     /translation="MSKLHRAASVVLAVLLKVASTRAEDAVELTPPVAQSSTDVAYPA
                     GADGDAAVVLELVIEKDGSVSSASVVEGEPPFAEQARTAALAWRFAPARRGDVPVAAR
                     IRTQVAFHREEELPAVAAPIEPRPVPATSLPSSALPSAPEPMVEVTVLGSRREIGQTT
                     LSKSDVRQMPGAFGDAFRAIEALPGVAPVASGLPYFYVRGAPPNNAGYYLDGIRIPFL
                     FHLGLGQSVVHPGLIDHVDFFSSAAPARYGGYAGANIAGQTREPARELHGEANLRLVD
                     AGALLEAPFAEGRGSALVAGRYGYPGPIIGALNNDLKIGYWDYQARTSYELGARDTLG
                     IFAFGAHDYVASRSPSGDPEARKKLVEQLVSDFHRLDLRYDHALTDGHVRLALTGGYD
                     SQGAEPTYITDKSLGARLFVEQRFGQSLRFRAGTEARLDQYRIRITRQGPGEPTVPQS
                     ANRPPTNLSAGLHFDLVWRIAPRVEIVPGARFDVYASSRLEEAPSTRHVRTILPAADP
                     RLSVRVSLTEKVTWLASFGLAHQYPSLRVGDIPAAIVSVPGFPFGDRKLQSAAQSSQG
                     IEIALPAELAFSATGFYSYFWGLTDLSASCFQLEPEMRERPTTPEPIVPPYVCPNNEP
                     VTGRAYGIELMLRRPFSKRLSGALSYTLSRTTRRAHFLTPSGTEDITTVPSEFDRTHV
                     FNAVLGYELGRRWRIGGRMLFYSGTPYSKLSGSLAVPPYNAYRNPAFYRLD"
BASE COUNT          526 a          997 c          968 g          552 t
ORIGIN      
        1 ctccaagaac aagcctaaca aggtcgactg cgcgatcgcg aaggtgctac ccaacgccaa
       61 ggtcagcgcg acgccgatgc ggaaggtcgg tcgcctagcc tcgggcgagg tgatcgaggc
      121 aactcctggc atgaaggtgc acaaggttgg gcgcacgacg agctacacga ccgggatcgt
      181 ccaggacgtc aacgccgacg tgacggtcgc ctacgaggtg ggcgccatca ccttccagga
      241 ccagatcgtc atcattggtg acgccgggtc attctccgac tccggcgatt caggatccat
      301 gatcgtcgac cgcgcgactc ggcgcccgac ggcgctcctt ttcgccggtt ccgcgagcca
      361 tacgatcgcg aaccacatgc aagacgtcct cgacgccttg gccgtggaga tcgtccggta
      421 gcggctcgct ccaagctcgg cgcggggcgg tgttggggct aagcttcggc atggccgagg
      481 accactccga cgctctcgca ctcaaacgac gtctctcgcc gctgcttcta cccctgccag
      541 gggtgactgg agtcggcgtt cgaagtggcc gcgtctgcgt gtaccttgcc agtgacgacg
      601 aggatactcg gcgacgggtc gagcagaccg tgcagtctga agcgcccggc gcctccgtgc
      661 tattcgaggt catgggtagg gtcgtgaagc agtaggccga gtgctgcggg cgcagcccat
      721 cgcccccatc ctttagggag gacgaaggac gggaaaggcg ggctggccac gaccgctgcc
      781 gccagagggc gcgcaatgaa ggaagtgcgt gcctcgagtg aataggggca agcgagcccc
      841 gtatgtcgaa acttcatcgc gccgcgagcg tcgtgctcgc ggtgttactc aaggttgcgt
      901 ccactcgggc ggaggacgcc gtcgagctca ctccgcccgt cgcgcagagc agtacggacg
      961 tggcgtatcc ggctggcgcc gacggcgacg ccgctgtcgt gctcgaactg gtcatcgaaa
     1021 aagacggctc cgtctcgagc gcgagcgtgg tcgaaggcga acctccgttc gcggaacagg
     1081 ctagaacggc ggcgctcgct tggcgtttcg ctcctgctcg acgtggcgat gtgcccgtgg
     1141 cggcgcgcat tcgcacccag gtcgcgttcc atcgagaaga agagcttccc gcggttgcgg
     1201 cgccgataga acctcggccg gttcccgcta catcgttgcc gagttcggcg ttacctagcg
     1261 ctcccgagcc gatggtcgaa gtcacggtac tcggttctcg ccgtgagatc ggccaaacca
     1321 cgctttccaa gagcgatgtg cgtcaaatgc caggcgcgtt cggcgatgcc tttcgggcta
     1381 tcgaagcgct gcccggagta gcaccggtcg cgagcgggct gccctacttt tatgtgcggg
     1441 gagcgccgcc caacaacgcg ggctactacc tcgacggcat tcgtatcccg ttcttgtttc
     1501 acctggggct cgggcagagc gtcgttcatc cgggactcat cgaccacgtc gacttctttt
     1561 cgagcgcggc tcccgcgcgc tacggcggtt acgccggggc caacatcgcc gggcaaacgc
     1621 gcgagcctgc gcgcgagctg catggtgagg cgaaccttcg gttggtcgac gcgggtgcac
     1681 tgctcgaggc accgtttgcc gaaggccgag ggagcgcgct cgttgcaggt cgatacggct
     1741 accccggccc aatcatcggc gctttgaata acgatctgaa gatcggatat tgggactacc
     1801 aggcgcggac cagctacgag ctcggtgctc gcgacacgct cgggatcttc gcgtttggtg
     1861 cgcacgacta tgtcgcctcc cgttccccgt ctggcgatcc ggaagcgcga aagaagctcg
     1921 tagagcagct cgtgtccgac tttcaccggc tcgacctccg ctacgatcac gcgctgacgg
     1981 acggacacgt acggctcgcg ctcaccggag gctatgactc tcagggagcc gagccaacct
     2041 acatcaccga caagtcgctc ggcgcgcgtc tcttcgtcga acaaaggttc ggccagagcc
     2101 tgcgttttcg cgcggggacc gaagcgcggc tcgatcagta tcgcattcgc atcacgcgcc
     2161 aggggccggg cgaaccgacg gttccccaga gcgcgaaccg tcctcccacc aatctcagcg
     2221 ccggcctcca cttcgacttg gtctggcgca tcgcgccgcg cgtcgaaatc gtccccggag
     2281 cgcgcttcga tgtgtacgct tcatcgcgtc tcgaagaggc tccgagcacc cgccacgtga
     2341 gaacgatcct gccggcagcc gacccgcgtc tctcggtgcg cgtaagcctc acggagaagg
     2401 tgacgtggct cgcgagtttc ggactcgctc atcaatatcc gagtttgcgt gtgggcgata
     2461 ttcctgccgc gatcgtttcg gtgccgggtt ttcctttcgg cgatcgaaag ctgcaaagcg
     2521 ccgcgcagtc gagccagggc atcgagattg cgctgccggc cgagctcgct ttcagcgcta
     2581 ccgggttcta ttcgtatttt tggggcctca ccgacttgag tgcgagctgc tttcaactcg
     2641 aacccgagat gcgcgagcga ccgacgacgc cggagccgat cgtgccgccc tacgtgtgcc
     2701 ccaacaatga accagtcacc ggccgggcct acggaatcga gctgatgctg cgtcggccct
     2761 tctcgaagcg cctgagtgga gcgctgtcgt acaccctgtc gcgaactact cggcgtgcgc
     2821 acttcctcac gccaagcggt acggaggaca tcacgacggt gccgagcgaa ttcgaccgaa
     2881 cgcacgtgtt caacgccgta ctcggctacg agctcggccg tcgctggcgc atcggcggcc
     2941 gaatgctctt ctacagcggt accccctatt cgaagctcag tggcagcctg gcggtaccgc
     3001 cctacaacgc ctaccgcaac ccggcgttct atcgtctcga ttt
//
LOCUS       DASTKS010000674         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1447649, whole genome shotgun sequence.
ACCESSION   DASTKS010000674 DASTKS010000000
VERSION     DASTKS010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447649"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFQ35_13885"
     CDS             <1..147
                     /locus_tag="VFQ35_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET9931785.1"
                     /translation="DYTSAKKVPSDAWGNPFRLVCPAHRPDERYELMSDGPDGKPGGL
                     DRIE"
     gene            159..620
                     /locus_tag="VFQ35_13890"
     CDS             159..620
                     /locus_tag="VFQ35_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9931786.1"
                     /translation="MKYRSLMSRLRERTARLRRAYAVRPRGVTLFEVLIVVAILAMVA
                     GGVAAFALPKFKEAQKSQAETGARVMRTAIQQWQAANSESTCPTISQLVQEKHIDPGQ
                     STNDPWSQPYTFVCSDDEVTVISAGPDKKKGTKDDISVPKNARLTGEEDGK"
     gene            632..1336
                     /locus_tag="VFQ35_13895"
     CDS             632..1336
                     /locus_tag="VFQ35_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9931787.1"
                     /translation="MSRRFRARGLTLVEVLIVVVLIALLTGGTVMGPGLLKASRVRGA
                     ATMLVSAVRLAQNRTNTVGKPVRLVLDLDKRRAALEEADSVSFARQKGGIAGGALAAD
                     DAEKKARAETERLLEGPKPQRAQFKPLKELSDPNDPNQGRELGSGVQFLSVETDHDEE
                     PVTEGRAYVYFWPGGITERAIIRLKRTDSNEEGLAVLISSLTGRTLIQHGKGEFPPPR
                     EDPEGQGSDSYDQEPR"
     gene            1336..2130
                     /locus_tag="VFQ35_13900"
     CDS             1336..2130
                     /locus_tag="VFQ35_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9931788.1"
                     /translation="MSDSSVRGFTLLEVLVAVAILGLGLTMILSSQVGLFSSASRGEH
                     LTIATNLARCKMADLEVELLKKGYPVTDSKDDGTCCGDDNDPAYQCEWKIERVTLPDQ
                     ALDGGAMDASLGPFAALANLGNPSSGSGSSPLGAGNPLGALGGGLGPAPMGSGGPQSL
                     QGLASSIGSSAANSGIGPMVMGMVYPSLKPMLEASIRKITLRVKWKEGVNERDIIITQ
                     YVTDPQKAQLFDGGMLPGLGVGGGSGLGSGSGLGTGTPGSTGIFGR"
     gene            2127..2828
                     /locus_tag="VFQ35_13905"
     CDS             2127..2828
                     /locus_tag="VFQ35_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9931789.1"
                     /translation="MRRFRRGFTLLELLVAISILAMVSILIYSAFAAMRKSRDGLARI
                     QDRYREGRLAMEYIRRDLSAAYLSMHLPIDQRQLVQKTAFIGKRGTPTDRVDFNSFSN
                     VRRDRDAHESDQAEISYFGSPNPTDSSVLDLARRSTSRLDLDPQHGGRVEVLASDIDL
                     FELEYLDPMTGMWSEIWDSTQVTGQPNRLPYQVRVTLVLNAGKRSSSDGGRGRIRLMT
                     TVNLAIQQPLNFATL"
     gene            2825..>3743
                     /locus_tag="VFQ35_13910"
     CDS             2825..>3743
                     /locus_tag="VFQ35_13910"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HET9931790.1"
                     /translation="MKWSPFQRQRRTLTRALRRSLARRRRRRGVAFIMVLGALTILTV
                     MLTELQDESSAEFASALEARDALVAEYAARSAINLSRLLIATEPTIRTAVSPILGLLF
                     GGQVPQIPVWNFADRMLGPFNDKSGGKDFSDFSGMDLSLGKNLGFPGARFEVNIVDED
                     AKINVNAASRGSTFGQQQLMTQLMALMAPAQYTPMFEKRDADGNFSDRQNICAAIIDW
                     VDPDQDTMLCDPTSQTAQSMPPEDSYYQNLKKPYQRKNAAFDSLEELRRVRGISDDYW
                     NTFVDPDPAHPEKRLITVWGQSNAININSA"
BASE COUNT          687 a         1203 c         1211 g          642 t
ORIGIN      
        1 gattacacga gcgccaagaa ggtcccgtcc gatgcttggg gcaatccgtt tcgcttggtg
       61 tgccccgcgc atcgccccga cgaacgttac gaactcatga gcgacggccc cgacggtaag
      121 ccgggcggcc tcgaccgcat cgaatgaaag agaaagccat gaaatatcga agcctcatgt
      181 cacgtctgcg cgagcgaacc gctcgtttgc gccgggccta cgcagtgcgt ccgcgcggcg
      241 ttacgctgtt cgaggtgctg atcgtggtcg ccatcctcgc gatggtggcc ggtggcgtcg
      301 cggcgttcgc cctgccgaag ttcaaggagg cgcagaagag ccaggctgaa acgggagctc
      361 gcgtcatgcg caccgcgatt cagcagtggc aggcggcgaa cagcgaatcc acgtgcccga
      421 ccatcagcca gctcgtgcag gagaagcaca tcgacccggg tcagagcacg aacgatcctt
      481 ggtcccagcc gtacaccttc gtttgctcgg acgacgaggt caccgtcatc tctgccggcc
      541 ccgacaagaa gaagggcacc aaggacgaca tctcggttcc gaaaaacgcg cgcctcacgg
      601 gcgaagagga cggcaagtaa gccgcaggcg tgtgtctcgt cgattccgtg cccgcggcct
      661 cacgctcgtg gaggtcctga tcgtggtggt gctgatcgcg ctactcacgg gaggcacggt
      721 catgggtccc gggctcctga aagcgtcgcg tgtgcgcggc gccgccacca tgctggtgag
      781 cgcggtgcgg ctcgcccaga accggacgaa caccgttggt aagcccgtgc gcctcgtcct
      841 ggatctggac aagcgccggg cggcgttgga ggaggccgac tcggtcagct tcgctcggca
      901 aaagggcggt atcgctggag gcgcgctcgc cgccgacgac gccgagaaga aggcgcgcgc
      961 cgagacggag cgcttgctcg aaggaccgaa gccccaacgt gcgcagttca aaccgctgaa
     1021 ggagctctcc gatccgaacg acccgaacca gggtcgagag ctcggcagcg gcgtacagtt
     1081 tctttcggtc gagaccgacc acgacgagga gcctgtcacc gaaggtcgcg cgtacgttta
     1141 cttttggccc ggcggcatca ccgagcgagc catcatccgg ctgaagcgca cggacagcaa
     1201 cgaggagggc ctcgcggtgc tgatcagttc gctcaccggt cgcacgctga ttcagcacgg
     1261 caaaggcgag tttccgcccc cgcgcgaaga tccggaaggg cagggctcgg acagctacga
     1321 ccaggagcca cgctgatgtc ggactcgtcg gtgcgcggtt tcactctgct cgaggtgctc
     1381 gtggcggtag cgatcctggg gctcgggctc accatgatcc tgagctcgca ggtaggcctc
     1441 ttctcgagcg cttcgcgggg cgagcacctg accatcgcga cgaacctcgc gcgctgcaag
     1501 atggctgacc tcgaagtcga gctcttgaag aagggctatc ccgtcaccga ttcgaaggac
     1561 gacggtacgt gctgcggcga cgacaacgac ccggcttacc aatgcgagtg gaagatcgaa
     1621 cgcgtcacgc tgccggacca ggcgctcgac ggcggtgcga tggacgcttc gctcggtcct
     1681 ttcgcggcgc tcgccaacct ggggaacccg agtagcggtt cgggcagcag cccgctcggc
     1741 gctggcaatc cgctcggtgc gctgggcggc ggcttgggcc ctgctccgat gggctccggc
     1801 ggcccccaga gcttgcaagg actcgcctcg agcattggct cgagcgcggc aaattccggt
     1861 atcggcccga tggtgatggg catggtttac ccgagcctca agccgatgct cgaggccagc
     1921 atccgcaaga tcacgttgcg cgtgaagtgg aaagaaggcg tgaacgagcg cgacatcatc
     1981 atcactcaat acgtgaccga cccgcagaaa gcgcagctct tcgacggcgg catgcttccc
     2041 ggcttgggcg tcggcggcgg ctccggtctc ggcagtggtt ccggcctcgg cacgggaacg
     2101 cccgggagca cggggatttt tggtcgatga gacgctttcg gcgaggcttc actctgctcg
     2161 agttgctggt cgcgatcagc atcctcgcga tggtgtcgat tctcatctac tcggccttcg
     2221 ccgcgatgcg aaagagccgc gatggcctcg cccgcattca agaccgctat cgcgaggggc
     2281 gcttggcgat ggagtacatt cgccgcgacc tctcggccgc gtatctatct atgcacttgc
     2341 cgatcgacca gcgacagctc gtccaaaaga cggcgttcat cggcaagcgc gggacgccga
     2401 cggatcgcgt cgatttcaac tccttctcga acgtgcgccg cgatcgcgac gcgcacgagt
     2461 ccgaccaggc cgagatttcc tatttcggct cgcccaaccc caccgatagc agcgtgctcg
     2521 atctagcgcg ccgttcgacc tcgcgtctcg acttggatcc gcagcatggc ggccgcgtcg
     2581 aggtcctggc gagcgacatc gacctgttcg agctcgagta cctcgatccg atgacgggga
     2641 tgtggtcgga gatttgggac agcacccagg tgacggggca gccgaatcgg ttgccgtatc
     2701 aggtgcgcgt cacgctggtg ttgaacgcgg gtaagcgctc gagctccgat ggtgggcgcg
     2761 gtcgcattcg cttgatgacg accgtgaacc tcgccatcca gcaacctttg aatttcgcaa
     2821 ctctatgaag tggtctccgt tccaacgcca gcgacggacg ctcacgcgag cgttgcgacg
     2881 gtcgctcgcg cggcgacggc gtcggcgcgg cgtcgccttc atcatggtgc tcggcgcgct
     2941 cacgattttg accgtgatgc tcaccgagct tcaggacgag agctcggccg agttcgcgag
     3001 cgcgctcgaa gcgcgcgatg cgctcgtcgc ggagtacgcg gcgcgcagcg cgatcaactt
     3061 gtcccggctg ctgatcgcca ccgaaccgac catccgcact gccgtctcgc cgattctggg
     3121 tctcttgttc ggcggccaag tgcctcaaat cccggtctgg aacttcgccg atcgcatgct
     3181 cggtcccttc aacgacaaga gcggcggcaa ggatttctcg gacttttcgg ggatggatct
     3241 ctcgctcggc aagaacctcg gcttcccggg cgctcgcttc gaggtgaaca tcgtcgacga
     3301 ggacgcgaag atcaacgtca acgcggcctc ccgtggcagc acatttggcc agcagcagct
     3361 gatgacgcag ctgatggcgc tgatggcgcc ggctcagtac acgccgatgt tcgagaagcg
     3421 tgacgcagac gggaacttct ccgaccgcca gaacatctgc gccgccatca tcgattgggt
     3481 cgatccggac caggacacga tgctctgcga cccgacgagc cagacggcgc agtcgatgcc
     3541 gcccgaggac tcgtactacc agaacctcaa gaagccctat cagcggaaga acgccgcttt
     3601 cgacagcctc gaagagctgc ggcgcgtgcg cggcatctcc gacgattact ggaacacctt
     3661 cgtcgacccc gaccccgccc atccggaaaa gcggctgatt acggtttggg gacagtcgaa
     3721 cgcgatcaac atcaacagcg cgt
//
LOCUS       DASTKS010000675         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_108515, whole genome shotgun sequence.
ACCESSION   DASTKS010000675 DASTKS010000000
VERSION     DASTKS010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108515"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VFQ35_13915"
     CDS             complement(<1..1379)
                     /locus_tag="VFQ35_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9931791.1"
                     /translation="MSLERIQGLFERFRREIAASTTPDAPPITLPRVLALELFESMLL
                     ARHLDRVAHELRASGHGHYTICSSGHEANVVLGRLTTPEDPSLVHYRSAALQVERARR
                     VPEIDPIADIVLSLVASSEEPASGGRHKVFGNRRLGIIPQTSTIASHLPRAVGLAFAL
                     ERKARLGLAPAKDSIVLVSFGDASINHSTALGAFNAAGWVAHQNLPCPVLFACEDNGL
                     GISVRTPPGWVRARLEALPHVRYFEADGSDIDASFRAAERAIDYCRNTRRPAVLHSSC
                     VRLLGHAGSDVDTSYRSPQELEEAAERDPLRRCALSLLAAEYASPAELLELDRRTGER
                     VDAARERAKQSPRLTSRGAVMSALARSIDPRANSSLAKRAPGNDRLTLAQGINRALTE
                     MLSEQPEALVFGEDVAKKGGVYGVTKGLLDRFGPTRVFNTLLDEQTILGLALGASTLG
                     LLPIAEIQYL"
     gene            1585..>3529
                     /locus_tag="VFQ35_13920"
     CDS             1585..>3529
                     /locus_tag="VFQ35_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931792.1"
                     /translation="MRLALLVGSAYEKNGRVASIPSAEIDEGLIERRLCEPDADFKVL
                     RFAAERGLAERIEQRLVAQTEPITELLVYFSGYSVLSEERGPALLLDGERLGTLSLTR
                     LRNLFLVHSTSSCLIVDAAAVVDANQSLGDVASAIGATLTSNAPSISALVAVRNSAVQ
                     DSFGGSAFTGMMLTALDWLAASRDEAQAIHMGELYSALSSDESAWKEIPAAGLFGDAP
                     AGFTILPAAAVVAATPPSARAPSPSFETVRPLDEVLGHPTLPPEEPKTQPGVAAFDAP
                     DEITPTHPHAPSSMEEPPLPSFELAGEHDADAPLPSFGLDESTEPHRLPLASEDGSGT
                     RLVPKATVLGVLAEGEQASSDASFESALSDHSQERARHSEPVESASATEAEHAEESDD
                     PDEIAVRELEAALTRTRDTSERATILARLARLLARVGRGERASVLFVEATQRDPLNIE
                     VLMLRGEWAEAARDTANLMHAAEMWLGVAPNDVRALILLGKAAERAGDARRSIDARLR
                     AAEHGECSPEDRRRALRAAARLVRSELADFDWAEKLDECARTGARLDAEESSQAAPLA
                     SEPLREPRVVAEPEREQYAAPPEAAESAPLASRELAIDAHPPDEFFAKDTAQAAHPAH
                     FTERHVTERFPDAISGAYPAPANA"
BASE COUNT          582 a         1163 c         1205 g          579 t
ORIGIN      
        1 agatactgga tctccgcgat cggcaagagc ccgagggtgc tcgcgccgag cgccagaccc
       61 aagatcgtct gttcgtcgag cagcgtattg aagacgcgcg tcgggccaaa ccgatcgagc
      121 aggcccttgg tcacgccgta gacgccgcct ttcttggcga cgtcctcacc gaacaccaac
      181 gcttcgggtt gctcgctgag catctcggtc agcgcccgat tgatgccctg cgcgagcgtg
      241 agccggtcgt tgccgggcgc tcgcttggct aaactggagt ttgcgcgggg gtcgatcgaa
      301 cgagcgagcg cgctcatgac cgccccgcgg ctcgtgagtc gtgggctctg cttggcgcgt
      361 tcacgggccg cgtccacgcg ctcgccggtt cgtcgatcga gctcgaggag ctccgccggg
      421 ctcgcgtact ctgcggccaa cagcgaaagc gcgcagcgtc gcagcggatc gcgctccgcg
      481 gcttcttcga gctcttgggg ggagcgatag ctcgtgtcca cgtcgctgcc ggcgtgcccg
      541 agcaagcgca cgcagctcga gtgcaggacc gccgggcgac gcgtgttgcg gcagtaatcg
      601 atcgctcgtt cggcagcgcg gaagctcgcg tcgatgtcgc tgccgtccgc ctcgaagtag
      661 cgaacgtgag ggagcgcttc gagccgcgcg cgcacccagc ccggaggggt ccgcacgctg
      721 ataccgagcc cgttgtcttc gcaggcgaaa agcaccggac aaggcaggtt ctggtgcgca
      781 acccaacctg cagcattgaa agcgccgagc gcggtgctgt gattgatact cgcatccccg
      841 aagctcacga gcacgatgct gtccttggcg ggtgcaaggc cgagtcgtgc cttgcgctcg
      901 agcgcgaacg cgagcccgac cgcgcgcggt aagtgggacg cgatcgtgct cgtctgaggg
      961 atgatgccca gccgtcgatt gccaaagact ttgtgacggc cgccgcttgc aggctcctcg
     1021 ctcgacgcaa ccagggagag cacgatgtcg gcgatcgggt cgatctcagg gactcgtcgg
     1081 gcgcgttcga cttggagggc ggcgctgcga tagtgcacga ggctcggatc ctccggtgtg
     1141 gttagtctcc cgaggacgac gttggcttcg tgtccgctcg agcagatggt gtagtgaccg
     1201 tggccgctgg cgcggagctc gtgggctacg cgatccaggt gccgcgcgag cagcatcgac
     1261 tcgaatagct cgagggcgag cacgcggggg agggtgatgg gcggtgcgtc gggcgtggtg
     1321 gacgccgcga tttcgcgccg gaatcgctcg aaaagtccct gaatacgctc gagtgacatc
     1381 tcggatggag gagcgtaccc gactcgtggg gtagtcgcgc gagccaccag aaaaactgca
     1441 caaaaagtgg tcgcgagtct cggaatcgca cgggagatcg aaacttgcgc cgaagctgga
     1501 ttgacttaca ggcttcgatg tgagcgcgcc gctgggcagg tcgcagaatc gaaagtatgg
     1561 ttcacgttcc tcaaagcgat agtcatgcgc ctcgcgcttc tcgttggttc cgcgtacgaa
     1621 aagaacggca gggtcgcgtc gattccgtcc gcggagatcg acgaagggct catcgagcga
     1681 cgcctctgcg agccagacgc tgacttcaag gtcctgcgtt tcgccgcaga gcgtggttta
     1741 gcggagcgca tcgagcagcg tctcgttgcg caaaccgagc cgatcaccga gttgctcgtc
     1801 tacttctcgg gttattcggt gctctcggag gagcgtgggc cggcgctgct gctcgacggc
     1861 gagcgactcg gcacgctttc gttgacccga ctacgcaatc tgtttttggt gcattcgacg
     1921 agctcgtgcc tcatcgtgga cgcggccgcg gtcgtcgacg ccaaccagag ccttggcgac
     1981 gtcgcgagcg caatcggggc gactctcacg agcaatgccc catcgatctc cgccctcgtc
     2041 gccgtgcgca acagcgccgt ccaagattcg tttggcggct cggcctttac gggcatgatg
     2101 ttgacagcgc tcgattggct cgcggcgagt cgtgacgagg ctcaggccat ccacatggga
     2161 gagctctact cggctctgag ctccgacgag tcggcatgga aggagatccc ggcggcgggc
     2221 ttgttcggcg acgcgccagc ggggttcacg atcctgcccg cggcagcggt cgtggccgct
     2281 acgccgccga gcgctcgagc cccttcgccg agcttcgaga ccgtgcggcc cctcgacgag
     2341 gtactcggtc atccgacgct cccccccgaa gagccgaaga cgcagcctgg cgttgccgcg
     2401 ttcgacgcgc ccgacgaaat cacgccgact cacccgcacg ccccaagctc catggaggag
     2461 cccccgctgc cttcattcga gctcgcgggg gagcatgacg ccgacgcccc cttgccctcg
     2521 ttcgggctcg acgagagtac cgagccgcat cgcctgccgc tcgcgagcga ggacggctcc
     2581 ggcacgcgcc tcgtgccgaa ggcgacggtg ctcggcgtgc tggccgaagg cgagcaggcg
     2641 tcgagcgacg cctcgttcga aagcgcgctc agcgatcatt cgcaggagcg cgctcggcac
     2701 tccgagccgg tcgagagcgc atctgccacc gaagcggagc acgcggaaga gagtgacgat
     2761 ccggacgaga tcgccgttcg tgaactcgag gccgcgctca cgcgcacccg cgacacgagt
     2821 gagcgcgcca cgatcctggc tcggctcgcg cgcctgctcg cacgggtcgg gcgcggagaa
     2881 cgcgcctcgg tgcttttcgt ggaagctact cagcgggacc cgctgaacat cgaagtgttg
     2941 atgcttcgcg gcgagtgggc ggaggccgct cgggatacgg cgaacctgat gcacgctgcc
     3001 gagatgtggc tcggcgtggc accgaacgac gtccgcgcgc tgatcttgct tggcaaggcc
     3061 gcggagcgcg ccggcgacgc gcgccgttcg atcgacgcac ggctgcgcgc ggccgagcac
     3121 ggcgagtgca gccccgaaga tcgcagacga gctctccgcg ccgccgcccg cttggtgcgc
     3181 tctgaactcg ccgacttcga ctgggccgaa aagctcgatg agtgcgcccg caccggggcg
     3241 cgtctcgacg ccgaagagtc ttcgcaagcg gctccgctcg cttcggagcc actccgggag
     3301 ccgcgggtgg ttgcggagcc tgaacgagag caatacgccg ccccgccgga ggcagcggaa
     3361 tcggctccgc tcgcgtcgcg cgaactcgcc atcgacgcgc atcctcccga cgagttcttc
     3421 gccaaagaca cagcgcaagc agcgcacccc gcccacttca ccgagcggca cgtgaccgag
     3481 cgcttcccgg acgctatctc cggggcatat cctgctcccg cgaacgcac
//
LOCUS       DASTKS010000676         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_331052, whole genome shotgun sequence.
ACCESSION   DASTKS010000676 DASTKS010000000
VERSION     DASTKS010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331052"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFQ35_13925"
     CDS             <1..523
                     /locus_tag="VFQ35_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931793.1"
                     /translation="IKAAPRSSSCDLATAVFIAEPDNEEESELAVLCQHGGRCGRLQV
                     PATWISDGFDVAQVKEVTVLASVAGGVVRVRSSRDHGKTWTPATVALDAESAGIPLAD
                     AKQVHLQPVGERLLLWVTGDGAQRSYPLVVSEDFGASFRGAELHTVPTRGVSRAAAPR
                     ASAMPGSVSLRAN"
     gene            complement(625..>2582)
                     /locus_tag="VFQ35_13930"
     CDS             complement(625..>2582)
                     /locus_tag="VFQ35_13930"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9931794.1"
                     /translation="DAIALLPNWVTALSMRAQLLADGGYRAAAAEAYERLAQAAHALP
                     LKAAAFKHAAEQLRRERPAEDEGLNTASNASLLPVVDPNRLRLNLERALDADPNDEDV
                     LDMLIDSYAQQRLDGELGELFLRRLEGAAPDVRQKLMLRWSQACSSMGSSERAFELVR
                     EVLKGNPDSAGALEQLASLTKDTAEREQTLLQLIRVAPTPAQQAAAYKQLGDFYHRES
                     PQPARAIKSYQEALRRKPDDGEALRALVEVQLAAGDVTSAKSTVDAFAQRSNTDAERR
                     AVAISRAAITAAADPNAGEAELQQLLADRPYDSVVLEELALVYVRANHTEKLRLLDER
                     VRQQAERELPAGAHVAAYLSALATLAKLRTDSSAFVLVSALSNLYHGVASGLDARGSR
                     AVSRALDADLAPHPLSESLRTLLSKTSEALEEAFPLNIADLNPLPLKDARLVASFEMK
                     AKAVGIAAPELFVVNSAPYLCQVTHHPSRVFLGSGWVEHAASLQDFVVWRSLKLLQAR
                     VGAVAHSSPESTALRLDALLSAYTDPGDTLVPNASGPNRLRAVLHPLLPKDPTLGALA
                     ATSVEELSQSELEVGDAIALWADRSALLATGEPRIALLAVCLMHGESGFTTPAALLDA
                     VARQPSARRLLVSLLDPGFVRAHRSIH"
BASE COUNT          428 a          811 c          885 g          458 t
ORIGIN      
        1 catcaaggct gcgccgcgct cgagctcgtg tgatctagcc accgcggtgt tcatcgcgga
       61 acccgacaac gaagaggaga gcgagttggc cgtgttgtgt caacacggcg gacgatgtgg
      121 ccgacttcaa gtgcccgcca cctggatctc cgacggcttc gacgtggctc aagtgaagga
      181 agtcaccgtg cttgcttcgg tggcgggcgg cgtggtgcgg gtccgttcgt ctcgcgacca
      241 cggcaagaca tggactccag cgaccgtcgc cctcgacgcc gagagcgcgg ggatcccgct
      301 ggccgacgcc aagcaagttc acctgcaacc ggtcggagaa cgcttgctgc tgtgggtgac
      361 cggcgacggc gcccaacgca gctaccccct cgtcgtttcg gaggacttcg gcgcgagctt
      421 ccgcggggcc gaacttcaca cggtgcccac gcgcggcgtg tcgcgtgcgg ccgcgccgcg
      481 agcgtctgcg atgcccggct ccgtttcgtt gcgggccaac tgaagctcgg tgccgccagg
      541 gccgacccgg tgggcgggag ccgtgaattg tctcacgggg ctgggcccaa cccaacccct
      601 tacgacacgc cactgcgacg agcctcagtg gatgctgcgg tgagcccgga cgaaaccggg
      661 atcgagtaga gacaccagca gtcggcgcgc gctcggttgc cgcgctacgg cgtccagcag
      721 agccgctggc gtggtgaaac cggactcgcc gtgcatcaag caaacggcga gcagggcaat
      781 ccgtggttca cccgttgcga gcagcgccga gcggtcggcc cacagcgcga tggcgtcgcc
      841 cacctcaagc tcgctctggc tcagctcctc gacgctcgtg gcggcgaggg cgccgagagt
      901 tgggtctttg ggtaacagtg ggtggagcac ggcgcgtagg cggttcggac cggaggcgtt
      961 ggggaccaac gtgtcgccgg gatcggtgta ggcgctcaac aacgcgtcta agcgcagcgc
     1021 cgtcgactcc gggctcgagt gtgcgaccgc gccgaccctg gcttgcagca acttcagcga
     1081 tcgccaaacg acgaaatctt gcagagacgc ggcgtgttcg acccagcccg aacccaagaa
     1141 cacgcggctc ggatgatggg taacctggca caggtatggc gcgctgttca ccacgaacaa
     1201 ctcgggagcg gcaatcccca cggccttggc cttcatttcg aagctggcca ccaggcgcgc
     1261 atccttgaga ggcaatgggt ttaagtccgc gatgttgagt gggaacgcct cttcgagcgc
     1321 ttcgctggtc ttggagagca gtgtgcgcag cgactcggac aacgggtgcg gtgctaggtc
     1381 ggcatcgagc gcacgcgaca cggcgcgtga acctcgggcg tcgagtcccg aagccacgcc
     1441 gtgatacaaa ttgctgagcg cgctgaccaa tacgaacgca gagctgtcgg ttcgcagctt
     1501 ggccaatgtc gccaacgcgc tgaggtaagc ggcgacgtgt gctcccgccg gaagctcgcg
     1561 ttcggcttgc tggcgaacgc gctcgtcgag cagccgcaac ttttccgtgt gattcgcgcg
     1621 cacgtatacg agcgcgagct cttcgagcac cacggagtcg tacgggcgat ccgctaacag
     1681 ctgttggagc tcggcttcac ccgcgtttgg gtcggccgct gcagtgatcg cagcccgaga
     1741 aatcgcgacc gcgcggcgtt cggcgtcggt gttgctgcgt tgcgcgaacg cgtcgacggt
     1801 cgacttggcc gatgtgacgt ctccggccgc gagctggacc tcgacgagcg cacgcagcgc
     1861 ctcaccgtcg tccggcttcc ggcgtaacgc ttcttgataa gacttgatgg cgcgcgccgg
     1921 ctgtggtgac tcgcggtggt agaagtcgcc gagttgcttg tatgccgctg cctgttgcgc
     1981 gggcgttgga gccacgcgaa tgagctgtag caaggtttgc tcgcgttcgg ccgtgtcctt
     2041 cgtcagtgag gccaattgct cgagggctcc ggcgctgtcg gggttgccct tgagcacctc
     2101 gcgaacgagc tcgaaggctc gttcgctcga gcccatgctc gaacacgcct ggctccagcg
     2161 taacatcaac ttctggcgga cgtccggcgc ggcaccttcg agcctgcgca agaacaactc
     2221 cccgagttct ccgtcgaggc gttgttgtgc atacgagtcg atcaacatgt cgaggacgtc
     2281 ttcgtcgttt ggatcggcgt cgagggcgcg ctccaagttc aaacgcaggc gattcggatc
     2341 gacgacgggc agcaatgaag cgttggacgc ggtgttgagt ccctcgtcct ctgctggacg
     2401 ctcgcgacgg agttgctcgg cggcgtgctt gaacgcggcc gccttgagcg gtagtgcatg
     2461 ggctgcttgg gcgagccgtt cgtaagcttc ggctgccgcg gcgcggtaac cgccatcggc
     2521 gagcaactgg gcgcgcatcg acagcgcggt aacccagttt ggcaacaggg caatcgcgtc
     2581 gt
//
LOCUS       DASTKS010000677         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_331215, whole genome shotgun sequence.
ACCESSION   DASTKS010000677 DASTKS010000000
VERSION     DASTKS010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331215"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFQ35_13935"
     CDS             <1..271
                     /locus_tag="VFQ35_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931795.1"
                     /translation="EAASAAALGLADAFVFRPADAQYLVALRAMEAAQSVDEPEARLE
                     HYERAITAWGEYLRTAPAEDRWLGAARAHQKRCELARRALAETAP"
     gene            complement(275..1390)
                     /locus_tag="VFQ35_13940"
     CDS             complement(275..1390)
                     /locus_tag="VFQ35_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931796.1"
                     /translation="MPRDSFFGERIIWQGRPEVVKQPPFLKALAAVAFVTSTISLSFA
                     AVIALALSELPVSPLLFSVWSAVLGGLLIVLPRRWAERARYTVTESHVIVKYGPFSRS
                     IERNAISFARIFWNEDHPNTGDLEIVRAVPTGALRRRLRLRLAGLLAPDRVWAIVRGA
                     ADLAPAGHGVRPVGQRLDQGERVVWTARSRFKFRAFLPQAQREWLLLTLALFLIAGVV
                     RMTSRLVPTFERLSAGALPMKSVSFVALSLGVALALLLMLAMAGYLAYDACIRQGLLA
                     METRYLVTNKRVLIQRRGEELHLDREKIIDVIDAPAGDGLRDIFIVLDGPRARAAATS
                     GAFGEMARGPHLRPVFEAVEDAESVSRILREKQRQAA"
     gene            complement(1536..2627)
                     /locus_tag="VFQ35_13945"
     CDS             complement(1536..2627)
                     /locus_tag="VFQ35_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931797.1"
                     /translation="MKSIALSASLLLGVACAAAPPRPRVLAELDASRTSAAVLDAEQH
                     APQAYAKAEDLRRRAEESHPKDPAGSQILAEQALVAYERAVIQARLVRAEGRRGEAEV
                     RVTRADAELSALQKQEQAVRAETDDLELRARVLEEALPVAQSSPATKEREQARLEAAR
                     ALGLQARLLCASARLLEPARPALAQLTTELNAFSERLSKKGPAPIDDATRLRARCLGE
                     LTEVRRPKTLAEPARGSGDTLLAELSNARYAPARDDRGVVVTLRGVFARGATVAPAAE
                     ATLQALGRVASAHPEFPILVVLHTARAQTAGLTEQLEAVVGGLKQAGAKQIAAELAGN
                     AAPLVDPSRPSADERNARVEVVFVGPSST"
     gene            complement(2624..3208)
                     /locus_tag="VFQ35_13950"
     CDS             complement(2624..3208)
                     /locus_tag="VFQ35_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931798.1"
                     /translation="MKSSEAPSPSRHWTGSPCRVLALLLALGSSGTFALPAAADDSAR
                     AQAEASLKGLDAEAKDKELVAPLVARARKALERALGARRSGDHAHGSQLEALALELTD
                     AAKDLVRTHAAEVAAKSTEDKALDLETRVVRARALVEQAAARRGRASERLRGIEAERG
                     KPAPATSKTKKVPAATDPATAAPPVKVPVGGSKP"
     gene            3299..3784
                     /locus_tag="VFQ35_13955"
     CDS             3299..3784
                     /locus_tag="VFQ35_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HET9931799.1"
                     /translation="MATHARASWDEYFMAIAIEVATRSTCDRKHVGAVIVRDKMILTT
                     GYNGSIRGLAHCDDEGHMMEDGHCVRTVHAEANAIVQAACNGVRLEGADIYVTASPCF
                     GCFKLIANAGLKRIVFGEFYRDERIYKLSEQLGIALLHQPIAPAQAAVAAAVSAAKEG
                     A"
BASE COUNT          601 a         1281 c         1324 g          613 t
ORIGIN      
        1 ggaggcagcg tcggcggccg cgctcgggct cgcggacgcg ttcgtgtttc ggcccgcgga
       61 cgcgcagtac ctcgtcgcgt tgcgcgcgat ggaagcggct caatccgtcg acgagcccga
      121 agctcggctc gaacactacg agcgcgcgat cacggcgtgg ggcgagtacc tccgcacggc
      181 tccggccgaa gatcgctggc ttggtgccgc acgcgcacac caaaagagat gcgagctcgc
      241 tcgccgggcc ctcgccgaga cggcgccctg aggttcaggc ggcttggcgc tgcttttcgc
      301 gcaggatccg gctcacgctc tcggcgtctt cgaccgcctc gaacaccgga cgaaggtgag
      361 gtccgcgagc catctcaccg aacgcgccgc tcgttgcggc ggcgcgcgcg cggggcccgt
      421 cgagcacgat gaagatgtcg cgcaggccgt cgccggccgg agcgtcgatg acgtcgatga
      481 tcttctcgcg atcgaggtgc agctcttctc cgcggcgctg gatgaggact cgcttgttgg
      541 tcacgaggta gcgagtctcc atcgcgagca ggccctgccg gatgcatgcg tcgtacgcga
      601 ggtaccccgc catcgcgagc atcagcagga gtgcgagcgc gacgccgagt gacaaggcga
      661 cgaacgagac gctcttcatc ggcagcgcgc ctgcgctcaa gcgctcgaat gtcggtacga
      721 ggcgcgacgt catgcgaact acgcctgcga tgaggaatag ggcgagggtc aagagcaacc
      781 actcacgctg agcctgtggc aaaaaagccc ggaatttgaa gcgagagcgc gccgtccaca
      841 cgacccgttc gccttgatcg aggcgctgtc cgacggggcg aacgccgtgg cccgctgggg
      901 cgaggtctgc tgcgccgcgc acgattgccc aaacccggtc gggagcgagc aggcccgcca
      961 ggcgcaacct gagtcgtcgc ctcaacgctc ccgtcgggac ggctcgtacg atctcgaggt
     1021 caccggtgtt cggatgatcc tcgttccaga agatccgcgc gaagctaatc gcgtttcgct
     1081 cgatgctccg cgagaacggc ccgtacttca cgatcacgtg tgattcggtg acggtgtaac
     1141 gcgcgcgttc ggcccagcgc cgtggcaaga cgatgagcaa gccgccgagc acggcgctcc
     1201 agaccgagaa gaggagcgga ctcaccggaa gctcggacaa cgcgagcgca atgacggctg
     1261 cgaaggagag gctgatcgtc gaagtcacga aggcgaccgc ggccagcgcc ttcaagaagg
     1321 ggggctgctt aacgacctca gggcgaccct gccaaatgat ccgctcgcca aagaaggaat
     1381 cgcgcggcac cggcaacctc acttacgcag ttacgaccaa tccttccata acttgcgctg
     1441 tgtgggctgc ttcgtccagc gttcgaccga gttcggcgtg tgggcggcgg tgctcaggga
     1501 gagcgcgacg tgcggaagcg cgccctccgc cgctctcacg tcgagctcgg gccgacgaac
     1561 acgacctcga cgcgggcgtt cctttcatcg gccgagggac ggctcggatc gacgagggga
     1621 gctgcatttc cggcgagttc tgcggcaatt tgcttcgcgc cggcttgctt caaacccccg
     1681 accaccgcct cgagttgttc cgtcagccct gcggtctgag cccgggccgt gtgaagcacc
     1741 acgagaatcg ggaattcggg atgagcgctc gcgacacgcc cgagcgcctg cagtgttgct
     1801 tcagcggcgg gagcaacggt ggcacctcgg gcgaagacgc cgcgcaaggt gacgaccact
     1861 ccacggtcat cacgggcggg agcgtagcga gcattcgaga gctccgcgag cagtgtgtca
     1921 ccgctaccgc gggctggttc ggcgagggtt ttggggcgtc gaacctcggt gagctcgccg
     1981 aggcagcgcg cccgcagtcg cgtcgcgtcg tcgatcggcg ctggcccctt cttcgaaagt
     2041 cgctcggaga aggcgttcag ctcggtcgtg agttgcgcca gcgctggacg tgccggctcg
     2101 aggagacgag ccgaggcgca cagcaagcgg gcttggagcc ccaacgccct cgcggcctcg
     2161 aggcgagcct gctcgcgctc tttggtagcc ggcgaactct gtgcgaccgg gagcgcctct
     2221 tcgaggactc gggctcggag ctccaggtcg tcggtctcgg cgcgcaccgc ctgttcttgc
     2281 ttctgcaacg ccgaaagctc ggcgtcggcg cgcgtgactc gcacttcggc ctcgccccgc
     2341 cgcccctccg ctcgcacgag gcgcgcctgg atgaccgctc gctcgtatgc gacgagcgct
     2401 tgctcggcga gaatctgcga cccggcgggg tccttggggt ggctttcttc ggcgcggcga
     2461 cggaggtcct ccgccttggc gtacgcctgc ggggcgtgct gttcggcgtc gaggaccgcc
     2521 gcgctcgtgc gcgacgcatc caactccgcg agcactcggg gccgcggggg tgcggcggcg
     2581 caagcgacgc cgagcagcag cgaggcagaa agcgcgatgc tcttcatggt ttgctcccgc
     2641 cgaccggcac cttgactggc ggcgccgccg tcgccggatc ggtcgcggcg ggcaccttct
     2701 tcgttttgct cgtggcgggc gcgggcttgc cgcgttccgc ttcgatcccg cggaggcgct
     2761 cgctcgcccg gccccgacgc gcggcggcct gttcgacgag tgcgcgcgcg cgaacgaccc
     2821 gcgtctcgag gtcgagcgcc ttgtcctccg tgctcttcgc cgcgacttcg gccgcgtgcg
     2881 tccgcaccag atctttggcg gcatcggtga gctcgagggc gagcgcttcg agctgactgc
     2941 cgtgcgcgtg gtcaccgctg cggcgggcgc ccagagcgcg ctcgagcgcc ttgcgggccc
     3001 gggcgacgag cggtgcgacg agctccttgt ctttcgcctc ggcatcgagg cccttcagcg
     3061 acgcctcggc ttgggcgcgg gcgctatcgt ccgcggcggc cggcaacgcg aaggtgccgg
     3121 aggagcccaa tgccagcaga agcgcgagaa cccggcaggg agaccctgtc cagtgccggc
     3181 tcggcgacgg agcttcactc gatttcacgc ccagaccaag ccatgcactc gtcgttgggt
     3241 caaggctctc gtgccttggg cggggccgcg ctatacggcc tggggagcct ttttacacat
     3301 ggcaactcac gctcgcgcaa gctgggacga atacttcatg gccatcgcta tcgaagtcgc
     3361 aacgcgttcg acgtgcgacc gaaagcacgt cggagccgtg attgttcggg acaagatgat
     3421 cctgacgacg ggctataacg gctcgattcg cggcctcgca cactgcgacg acgagggcca
     3481 catgatggag gacgggcact gcgtccgcac cgtgcacgcc gaggccaacg ccatcgttca
     3541 agccgcgtgc aacggtgtgc gcctcgaggg cgccgacatc tacgtgacgg cgtcgccctg
     3601 cttcggctgc ttcaagctca tcgccaacgc aggactcaag cgcatcgtgt tcggagagtt
     3661 ctaccgagac gagcgcatct acaagctgag cgagcagctc ggcatcgcgc tcttacatca
     3721 gcccatcgct ccggcgcagg ccgcggtcgc cgctgccgtc tctgcagcga aggagggcgc
     3781 ttgagcgggc tcaaggtcgc cgtggtcgcg ggcggcccc
//
LOCUS       DASTKS010000678         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_776891, whole genome shotgun sequence.
ACCESSION   DASTKS010000678 DASTKS010000000
VERSION     DASTKS010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776891"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFQ35_13960"
     CDS             complement(<1..613)
                     /locus_tag="VFQ35_13960"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9931800.1"
                     /translation="MNAYLREPVREDALYALGQTLKRHDYDFVTVTPSTHARVLGRET
                     RLGATLRDAFGWNRPFAKSALPAEIMELASAAGVLVELEHGFRLNVRFATLGGEIFAH
                     SSFPTVQDDAVFFGPDTYRFVRFVCRSLGTARSLVDIGCGSGAAGIFAARHVETVIST
                     DINPTALAFTRVNARLAGVTSVVRHSDVLRSVIEPVDVVIANPP"
     gene            complement(610..1362)
                     /locus_tag="VFQ35_13965"
     CDS             complement(610..1362)
                     /locus_tag="VFQ35_13965"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9931801.1"
                     /translation="MLELENEFVEQERAHIRHRALFAPRDPDAFVSWFEQLRQSGPGQ
                     GDSLFPWLATHASREEMRWFLEQEVAGEAGFDDLVALTQVKLPATAKLEMARNYWDEM
                     GRGHLGGMHGPMLSRLAKALNLHPSPNTVVWESLALGNLMIALAVNRRYTYHSVGALG
                     VIELTAPGRAEYVNAGLKRLGVDGTARRYFALHATLDVKHSEAWNREVLRPLVAEKRE
                     VAVAIAEGALMRLSAGARCFERYRRELGLGVS"
     gene            complement(1646..>2614)
                     /locus_tag="VFQ35_13970"
     CDS             complement(1646..>2614)
                     /locus_tag="VFQ35_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9931802.1"
                     /translation="QNSSGGTGSGGRSNGGTSGSSGGAPTSTGGRSQGGTGGKGGSAG
                     SSGSSGANSGGSNSSGGKANAGGGGGGASAGAAGTGTGSDPVKVWIAGDSTVQNCSSA
                     CPCGWGSQFDALFNSNVTVQNSAVGGRSVQTWLYDANVSSTMTNGECALSSTTYNARW
                     TAMTNASTGMKTGDYLFIQFGINDGDTTCPRHVGLTLFKDYLGKMAQAAEARGAQPIF
                     LTPVSAISCNSSGKAVGTRGAFVTATIEAGTTYDVPVIDLHQLSVDLYNSLGLCPNNS
                     DYTMGKVGDFFCEDHTHFEAAGAKQIAQIIAKALRDQKIGLASYLL"
BASE COUNT          443 a          830 c          874 g          467 t
ORIGIN      
        1 agggcgggtt cgcgatgacg acgtcaacgg gctcgatgac gctgcgaagc acgtcactgt
       61 gtctgacgac ggaagttacg ccggcgagcc tcgcattcac ccgcgtgaag gcgagcgcgg
      121 tcgggttgat gtcggtcgag atcaccgttt ccacgtgacg cgcagcgaag attcctgcgg
      181 cgcctgagcc acaaccgatg tcgacgagcg agcgggcggt accgagcgag cggcacacga
      241 agcgcacgaa gcgataggtg tctgggccga agaacacggc gtcgtcctga acggtcggaa
      301 atgacgagtg ggcgaatatt tccccgccga gcgttgcgaa ccgcacgttc agccggaacc
      361 cgtgctcgag ctccacgaga acccccgccg cgctagcaag ctccatgatt tctgccggta
      421 gagccgactt tgcgaagggg cggttccaac cgaaagcatc gcgcagtgtg gcgccaagcc
      481 gagtctcgcg cccgagcaca cgggcatgtg tcgacggcgt aaccgtaacg aagtcgtagt
      541 cgtgtcgctt gagcgtttgc ccgagagcgt agagggcatc ttctcgcacc ggctcccgca
      601 ggtaggcgtt catgagacgc cgagccccaa ctcccggcga tagcgttcga agcagcgcgc
      661 gcccgccgac aaccgcatca gcgcgccttc tgcaatcgcg accgctacct cccgcttttc
      721 ggctaccaag ggccgcaaaa cctcgcgatt ccaggcttcg gagtgcttca cgtcgagcgt
      781 ggcgtgcaga gcgaagtagc ggcgcgcggt tccgtcgacg ccgagccgtt tgagcccagc
      841 gttcacgtac tcggcgcggc ctggcgcggt gagctcgatg actccgagcg cgccaacgga
      901 gtgataggtg tagcggcggt tcaccgcgag cgcgatcatg agattgccga gcgcgagcga
      961 ttcccaaacg acggtattcg gcgacggatg aaggttcagc gccttcgcaa gccgcgacaa
     1021 catgggccca tgcatcccgc ccaagtgccc ccgacccatt tcgtcccagt agttccgggc
     1081 catttcgagc tttgcggtgg caggcagctt cacttgagtg agagcgacca agtcgtcgaa
     1141 gccggcttca cccgccactt cttgctcgag aaaccagcgc atctcctcgc ggctcgcgtg
     1201 ggttgccaac cacggaaaga gtgagtcccc ctgccccggt cccgactgcc tgagctgctc
     1261 gaaccagctt acgaaggcgt ccggatcgcg tggcgcgaac agcgcacgat ggcgaatgtg
     1321 cgcgcgctcc tgctcgacga actcgttttc gagctccagc atgaggagtt cggcccgcgc
     1381 cgagcggcgc cattcggagg acggcaatgc cggttcgagc cgtgcatggt tgaacccggt
     1441 gaggcgccgc tggaaacttt cgagcgcttc cgcagttgag gggagagctt cggccgatcg
     1501 catgacacgc ccagctgcaa tcgatgtgcc gcaagaccgg acgcttgctg cgaatcgccg
     1561 taaggcacgc gcggacgcga tcccccgttg ctgcgcgctt gcgcaccttg acgccccccg
     1621 gcccgaccgc accagcgcgt tggcgtcaga gcaggtacga agcgagcccg atcttctgat
     1681 cgcgcagggc cttggcgatg atctgggcga tttgcttggc gccagccgct tcgaaatggg
     1741 tgtggtcttc gcagaagaag tcgccgacct tgcccatcgt gtagtcgctg ttgttcgggc
     1801 acaagccgag ggagttgtag aggtcgacgc tcagctggtg caaatcgatg acggggacgt
     1861 cgtaggtggt accggcttcg atcgtggcgg tgacgaaggc gccgcgggta ccgaccgctt
     1921 tgccgctcga gttgcaggag atggcgctca cgggcgtaag gaagatgggc tgcgcgccgc
     1981 gggcttcggc agcttgcgcc atcttgccga ggtagtcttt gaagagagtc aggccgacgt
     2041 ggcgcggaca agtggtgtcg ccgtcgttga tgccgaattg gatgaagagg tagtcgccgg
     2101 tcttcatgcc cgtgctggcg ttggtcatcg cggtccagcg cgcgttgtac gtggtgctgc
     2161 tgagcgcgca ctcgccgttc gtcatggtgc tgctgacgtt ggcgtcgtag agccaggtct
     2221 gaacgctgcg gccgccgaca gcgctatttt ggacggtaac gttggagttg aagagcgcgt
     2281 cgaactggct gccccaaccg cacggacagg cggagctgca gttttggacg gtggagtctc
     2341 ccgcgatcca aacctttacg gggtcgctgc cagtgcccgt gccggccgcg ccagcgcttg
     2401 ctcctccgcc gccaccgcca gcgttcgctt ttccgccgct cgagttggag ccgccgctgt
     2461 tcgcgcctga agagcctgag gagccagcgc tcccgccttt gccgcccgta ccgccttggg
     2521 aacgcccgcc agtcgaagtg ggcgcgccgc cgctcgagcc cgaggtaccg ccgttgctgc
     2581 ggcctccgct tccggtgccg ccgctcgagt tttg
//
LOCUS       DASTKS010000679         5348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1448245, whole genome shotgun sequence.
ACCESSION   DASTKS010000679 DASTKS010000000
VERSION     DASTKS010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448245"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VFQ35_13975"
     CDS             <1..1660
                     /locus_tag="VFQ35_13975"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9931803.1"
                     /translation="ATAGTSPSEEVSGGSGGRATDPAPSRGGETSVASGGVAGGASIP
                     PADVNRVALHRLNNAEYDNTVADLLGVSSKPGAGFIDDEKLAGFDNIAAALAMSDARY
                     EQYFNAADSLVEQAFADDALRRRVLTCTPADAAQETDCARTILKAFAKRAYRRPIRDA
                     ELDRMLGLVSAARALGEDFTGAVKNAVKALLSSADFLYRVELDADPASVTPHPLSAHE
                     LASRLSYSLWRTMPDATLFQRADDGTLLQDSVLSAQLDRLLSDSRAQRFAEAFAGQWL
                     GLRALRSHQVDAATFPEWNEPLRAAMVDEGLQYFSEFLVGKRPMTEFLTADVNFVNGP
                     LAKLYGFSPPSGDKLVSVTNTSDQRRGFLGLASFLTLSSFAQRTAPTLRGKWVLENLL
                     CSPTPAPPPNVPELDTGGAANASSENIRDRLEQHRKNPTCAGCHKVLDPMGLGLENFD
                     AIGRFRDHYANGDAVDPSGMLPTGEAFAGLVELSGLVTKDERLLDCAAQKMMTYALSR
                     EVVESDAAYLRDIEAQWANDGMGLAALLKRIVLSDAFRLRRGEP"
     gene            1697..3097
                     /locus_tag="VFQ35_13980"
     CDS             1697..3097
                     /locus_tag="VFQ35_13980"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9931804.1"
                     /translation="MDRSNRRQFLRGAGIALTLPWLETLAHKEAKAETPRIQRYVMLY
                     FSNGTADFWRPASAGAGDAWALSPILEPLGKVKRYVSVLTNVSNYAPFGGHVEPSHSN
                     LGAATWTCVKPSGQNNANSGISVDQVIANRIGSATRLPSLQVGLSTLDSYTDGLPGQH
                     SRSMSWSSASQPLYKMVSPQAVFDRLVADSPTGGGMDDAVAARRRALKQSALDYVKDS
                     ASDLQKKLSKSDRDRLEQFLTATRSLEKRVADFAPAPVANACAMRSRPTEVYAVGNTG
                     DGYNRGVHSKLMIDLVTMAIRCDMTRVVSFMLDDARSDFVYDFLKERKFTDTGSTEGT
                     GTVGGYHGLQHAGDRNNGFATIGYWNAQQAAELAAALMAIDDGNGSNALDNTVIAFAS
                     GMKGGNHDAGELPIALIGGGGKTTSGTLLKTNQHVVFPNETRLADVHLTILQRVFGCP
                     EPSFGASSGVLPALLS"
     gene            3151..3744
                     /locus_tag="VFQ35_13985"
     CDS             3151..3744
                     /locus_tag="VFQ35_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931805.1"
                     /translation="MSVRGSQSSRAETFAESVQATLPSPEFREVYDTHFAFVWRAVAN
                     RGVPAAALDDVVQEVFLVVHRKLSEFEGRSSLRTWLAAIVRRVVADHMKKRGNRALGQ
                     ELSTELPAESGNEPAAQAERRAALLLAEELLATMSEVQREVFVLYELEQMTTREIAEL
                     TSTNENTVQTRLKAARRMFQEGVARHQARDTRNGGGT"
     gene            3741..4451
                     /locus_tag="VFQ35_13990"
     CDS             3741..4451
                     /locus_tag="VFQ35_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931806.1"
                     /translation="MTDLSPEARRILDLTREARTPSIDDKLRVARLLSRSVELTALSV
                     ASQASGAAAAKPTLFALSVKWLAGVVPIVVLAAAGYSYWPRPEPSVSAVDRVVVAPPA
                     QPPTVLESPASAVPAEATSVIAPVATVGSPPAARSDQKSRRPNREDTLNEELDLLHDA
                     QAAWRSGDATRALSLIAQHRKRFPKSALAPERDALSVLSLCASGRTAEAKAAAQRAFR
                     KGERSPLHAAVERSCIKD"
     gene            4561..>5348
                     /locus_tag="VFQ35_13995"
     CDS             4561..>5348
                     /locus_tag="VFQ35_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931807.1"
                     /translation="MRRVRLVTLLFFAFATRRAHATEPAPALKGEAPLPLELEWKAPP
                     ECPKADDIHAELSRIARVRPGRVPPTVVARVRIDKRSGRYHLVLKTTRDGEVGKRNLS
                     AAECGALGREVTLVLALTFGDGVALASEGEDEPPTADAGNSSAPVETKPEAPPKPLPV
                     APPSARAEAASPPAAPVTSLERTPRVAAFAGGGVLLRALPRLAARISLGAEVGSRKFW
                     LAPELSVVLPSHDSLARGVSARYDAAGGALAGCSALAESPRVAA"
BASE COUNT          939 a         1731 c         1788 g          890 t
ORIGIN      
        1 agccaccgcg gggacgtcac caagcgaaga ggtgtcgggc gggagcggtg gtagagcgac
       61 agaccctgcg ccgtcgcgcg gcggagagac ctccgtcgcg agcggcggag tggcaggcgg
      121 cgcgagcatt ccccccgccg acgtgaatcg cgtcgcgttg catcgcctca acaacgccga
      181 atacgacaac accgtcgcgg atttgctcgg cgtctcttcg aaaccgggag caggcttcat
      241 cgacgacgag aagctcgccg gtttcgacaa catcgcggcg gcgctcgcga tgagcgacgc
      301 gcgctacgag cagtacttca atgctgccga ctcgctcgtc gagcaggcgt ttgccgatga
      361 cgccctgaga aggcgcgttt tgacctgtac cccagccgat gccgcgcaag agacagactg
      421 tgcgcgcacc atcctgaagg cattcgcgaa gcgcgcgtac cgtcgcccaa ttcgcgacgc
      481 cgagctcgac cgcatgctcg ggctggtcag cgctgctcgc gctctcggcg aggatttcac
      541 gggcgccgtc aagaacgcgg taaaagcgct cttgtcttct gccgacttcc tgtaccgagt
      601 cgagctcgac gccgaccccg cctcggtgac gccgcacccc ctgagcgcgc acgagctcgc
      661 gtcgcggctc tcgtattcgc tttggcgcac catgcccgac gcgacgctct ttcagcgggc
      721 cgacgacggc actctgcttc aagacagcgt cttgtcggcg caactcgatc gactgctttc
      781 cgactctcgc gcgcagcgtt tcgcggaggc gttcgcgggg caatggctcg ggctgcgtgc
      841 gcttcgcagc caccaagtcg acgccgccac tttcccggag tggaacgagc cgttacgcgc
      901 cgccatggtc gacgaagggc tgcagtactt ttcggagttt ttggtgggca aacgcccgat
      961 gaccgagttt ctcaccgcgg acgtcaactt cgtgaacggg ccgctcgcga agctctacgg
     1021 gttttcgccg ccgtccggcg acaagctcgt ttcggtcacg aacacgagcg accaacgccg
     1081 aggcttcctc gggctcgcga gcttcttgac gctctcgtcg tttgcgcagc gcaccgcgcc
     1141 cacgctgcga ggtaagtggg tgctcgagaa cctgctttgc tcgccgacgc cggcgccgcc
     1201 gccgaacgtg cccgagctcg acacgggggg cgccgcgaac gcatcttcag agaacattcg
     1261 cgaccggctc gagcagcatc gcaagaaccc gacttgcgcg gggtgccaca aggtcctcga
     1321 tccgatgggc ctcgggctcg agaacttcga cgccatcggt cgcttccgag atcactacgc
     1381 gaacggagac gcggtcgatc cgagcgggat gttaccgacg ggcgaagcgt ttgcggggct
     1441 cgtcgagctc tctggcttgg tgacgaaaga cgagcgcttg ctcgattgtg ccgcgcaaaa
     1501 gatgatgacc tacgctctgt ctcgcgaggt ggtcgagagc gatgccgcgt acttgcggga
     1561 catcgaagcg cagtgggcga acgacgggat gggcctcgct gcgctgttga aacgcatcgt
     1621 tttgagcgac gccttcaggc tgcgccgagg agagccttga ggaatgacaa cgaacactca
     1681 cggagaatct cgccgaatgg accgttcgaa tcgtcgtcag tttctgcgag gcgccggcat
     1741 cgcgctcacg ctcccttggc tcgagacctt ggcgcacaaa gaagcaaaag ccgagacccc
     1801 acgcattcaa cgttacgtca tgttgtactt ttcgaacggc acggcggact tctggcgtcc
     1861 ggcgtcggcg ggggctggtg acgcctgggc gctctcgccc atcctcgagc cgctcggcaa
     1921 ggtgaagcgc tacgtcagcg tgctcaccaa cgtctcgaac tacgcgccgt tcggtggtca
     1981 cgtcgagccc agtcactcga acttgggcgc ggcgacgtgg acgtgcgtga agccgagcgg
     2041 ccaaaacaac gcgaacagcg ggatctcggt ggatcaggtg atcgcgaatc gaatcggaag
     2101 cgcgacgcgc ttgccgtcgc tgcaggtcgg gctctcgacg ctcgactcgt atacggacgg
     2161 gctgcccggg cagcactcgc gcagcatgtc ctggagttcg gcgtcgcagc cgctctacaa
     2221 gatggtgagt cctcaagcgg tgttcgaccg acttgtcgcc gactcaccga caggtggtgg
     2281 catggacgac gcggtcgccg cccggcgtcg cgccctgaaa cagagcgcgc tcgattacgt
     2341 gaaagacagc gcctccgacc tgcaaaagaa gctcagcaag agcgaccggg accggctcga
     2401 gcaattcctg accgcgacgc ggagcttgga gaagcgcgtg gccgatttcg cgcccgcccc
     2461 cgttgcgaac gcctgcgcga tgcgctcgcg gccgaccgaa gtttacgccg tcggaaacac
     2521 gggagacggt tacaatcgag gcgtgcactc gaagctgatg atcgacctcg tgacgatggc
     2581 gatccgctgc gacatgacgc gcgtcgtcag tttcatgctc gacgacgcgc ggagcgattt
     2641 cgtctacgac ttcttgaaag agcgcaaatt caccgacacg gggtcgaccg aagggaccgg
     2701 caccgtcggc ggctatcatg ggctccagca cgcgggcgat cgcaacaatg gcttcgcgac
     2761 gatcggctac tggaacgcgc agcaggcggc ggagctcgcg gcggcgttga tggcgataga
     2821 cgacggtaat ggtagtaacg ccctcgacaa caccgtaatc gctttcgcga gcgggatgaa
     2881 aggcggcaac cacgacgcgg gcgagctgcc catcgcgctc atcggtggcg gcggcaagac
     2941 gacgagcggc acgctgttga agacgaatca gcacgtcgtg ttcccgaacg agacacgcct
     3001 cgccgacgtg catctgacca tcctgcaacg cgttttcggc tgtccggagc cgagcttcgg
     3061 cgcaagttcc ggggttctcc ctgcgcttct gagttgaccg atgacagagc gcgcgcattc
     3121 gattcgcagc cgaaaggagc cggtgctcgc atgagcgtac ggggctcgca gagctcgcgc
     3181 gcggagacgt tcgccgagtc cgtgcaggct accttgccct cgcccgagtt ccgtgaggtt
     3241 tacgacacgc actttgcgtt cgtctggcgc gcggtcgcga atcgaggtgt tcccgcggcg
     3301 gctctcgacg acgtcgtcca agaggtcttc ctcgtcgtgc atcgcaagct ctcggagttc
     3361 gagggccgct ccagcctgcg cacctggctc gccgcgatcg ttcggcgggt cgtcgccgat
     3421 cacatgaaga agcgaggcaa tcgcgcgttg ggccaggagc tctccacgga gctgcccgcg
     3481 gagagtggca acgagcccgc cgcgcaggcc gagcggcgcg cggcgctact gctcgcggag
     3541 gagctgctcg ccaccatgag cgaggtccag agagaggtct tcgttctata cgagctcgag
     3601 caaatgacca ctcgagaaat tgcggagctt accagcacga acgagaacac cgtgcaaacg
     3661 cgcttgaagg ctgcgcgtcg gatgtttcaa gaaggcgtgg cgcggcacca agccagagac
     3721 acgcgaaacg ggggcggaac gtgacagact tgagtcccga ggcgcgacgg atcctcgatt
     3781 tgacgcgcga ggcccgcaca ccgagcatcg acgacaagtt gcgcgtcgcg cggctcttgt
     3841 cgcgaagcgt cgagctcacc gcgctcagcg tcgcatccca ggcgtcgggc gcagcggcag
     3901 caaagcccac gctcttcgcg ttgagcgtga agtggctcgc gggagtggtg ccaatcgtcg
     3961 tcctcgccgc ggctggatat tcttactggc cgcgacccga gccgtcagtg agcgcagtcg
     4021 accgagtcgt cgtggctccg ccggctcagc ccccgaccgt gctcgagagc ccagcgtcag
     4081 cggttccggc agaggcgaca tccgtcattg ccccggtagc gaccgtcggg agtcccccag
     4141 ctgcgcgctc cgaccagaag tcgcgccggc cgaaccgcga agatacgctc aacgaggagc
     4201 tcgacctgtt gcacgacgca caagcggcgt ggcgcagcgg ggatgctacg cgggcgctct
     4261 ctttgatcgc gcaacatcgg aagcgctttc cgaaatcggc gctcgccccg gagcgcgacg
     4321 ctctcagtgt gctcagcctc tgtgcgtccg gccgcacggc agaggcaaaa gcagctgcac
     4381 aacgggcgtt tcgcaaggga gagcgctctc cgctgcacgc cgctgtggag cggagctgca
     4441 tcaaggattg aggggcccag gtttcgggcg ggcggggcta cggaacgcga gcgggaaggg
     4501 gccattggcg cggtgcaagg gattggccca accgcgcgag cttgggtgta acctgggcgg
     4561 gtgcgccggg ttcgtttggt gacgttgctc ttcttcgctt tcgcgacccg gagagcgcac
     4621 gcgaccgagc cagcgccagc tctcaaaggt gaggcgccgc tgccgctcga gctcgagtgg
     4681 aaggctccgc ccgagtgtcc caaagcagac gacatccatg ccgagctttc gcggatcgct
     4741 cgcgtgcggc ctggaagagt gccccccacc gtcgtagcgc gtgttcggat cgataaacgg
     4801 agcggacgct accatctcgt gctcaagacg acgcgcgacg gtgaagtcgg taaacgcaac
     4861 ctctccgccg ctgagtgtgg tgcgctcggg cgcgaagtga cgctcgtttt ggcgctcacg
     4921 ttcggcgacg gggttgcgct tgcgtcggaa ggcgaggacg agcctcccac ggccgacgcg
     4981 ggtaactcct cggcgccggt ggagacgaaa cccgaagcgc caccaaagcc gcttcccgtg
     5041 gccccaccga gcgctcgcgc cgaagctgca tcgcccccgg ctgcgcccgt cacatcgctc
     5101 gagaggacgc ctcgagtcgc tgcgttcgcg gggggcggcg tgcttttgcg tgcgctgcct
     5161 cgcctagccg cgcgaatttc tctgggcgcc gaagtcggta gtcgcaagtt ttggcttgcg
     5221 cccgaactca gtgtggtcct tccgtcgcac gactcgttgg cgcgcggcgt gagcgcacgc
     5281 tacgacgcgg ccggaggagc gctcgcaggc tgttcggcac tagcggagtc tccacgcgtc
     5341 gctgcgtg
//
LOCUS       DASTKS010000680         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1670751, whole genome shotgun sequence.
ACCESSION   DASTKS010000680 DASTKS010000000
VERSION     DASTKS010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670751"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFQ35_14000"
     CDS             <1..445
                     /locus_tag="VFQ35_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931808.1"
                     /translation="IFFVFLTELARFGPLSGRHTLDHANQALSRTVRRVRQICDRDPA
                     VAPCTLTLMATDGETLVATHGGKELFVSTYKKRCVDRDRCASLSAACEGPSPDGRVNH
                     IILSSEPLHGENVWVPLEPGELIGVGADMRPRRQHIERCNLPLAG"
     gene            complement(541..1566)
                     /locus_tag="VFQ35_14005"
     CDS             complement(541..1566)
                     /locus_tag="VFQ35_14005"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET9931809.1"
                     /translation="MLLAAHAGACNAARPSNVGYDLVREPDREPVVAEKPKHRVPPDV
                     VQRARGPVRFKRLSDGSDVPEAQVATELLKADVVCAGEEHTRAAQHYGELWLFERLAE
                     RAPALGLELGVGFEMWASDYQRPLWDYQRGKIDQTTLLQRTQYEERWGYPFAYYSPIL
                     ERARRLRLPLVALNARAEITSKIAEGGVDALSPRQARSLPALDLDDAQHRADFERRMK
                     DHPGVTEQTLDRYYQAQVVWDESMAHTAARWVLEHAPMRRLLVLAGQAHCQERAIPNR
                     VERRGVRRVTNVLLAEGEPSPERRRGYEYALIVGQSEEAGDESESASESESEQNEGAD
                     PKSAPGE"
     gene            complement(1650..4136)
                     /locus_tag="VFQ35_14010"
     CDS             complement(1650..4136)
                     /locus_tag="VFQ35_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HET9931810.1"
                     /translation="MPVSQSDSAALSDDPERDRIVAEEESCLQRVTSHLAQNKQRVRA
                     ARDSAEYDARLLDLRDQIASARLEDIPPLVQEMERLQSLAAHLRETTESEVDPRSPYF
                     GRLVLEEGERKREVLIGRGTHLDTRENIRIVDWRDAPVSRLYYRYEEGEEYDEEFGGR
                     EVSGTVNVRRSLVITDAVLRRVGTPQGSFARTRDGSFRRLEATQTRLAGGAGSAIRAE
                     QHHRPGKLGTGKDRGEDKQLKEITPLIDRRQFELITRPDSGLVVIQGGAGSGKTTIGL
                     HRLAYLAFQDPRRFRPDRMLVIVFNAALARYINGVLPALGVNGVAIRTYEDFVSRLRQ
                     ALFPRLPSHYREDAPAVVTRLKKHPAMLHAIEAHVARFEAKLDVEVRAALAEEARTPG
                     AAATLLEGWDRSRKRIIAHRLHALSTRLEEAEMRQLSTNARVSLERTIKNGISAARDV
                     AQHWAELLSDRALIRKVYRDRAGGDFTDADLERAHTYCAARVSEVLDELIEKSERRAE
                     RREERGRDEEDEGKGRGRSRKSEDDDDDDSADPAPRVGVDGRDVEDRARLEPEDDTLI
                     LRLSQRLRGPLTRSATSKDPVIYEHILIDEAQDLSPVELAVVMNTVNRSESVTLAGDI
                     QQRLLLDNGFTNWKEVLGELGLSHVEVEPLKLSYRSTHEIIAFARHVLGPLVSPDAPE
                     ATRHGAPVELFAFGHMGDSVAFLADALRDLLSEEPNASIAVVTRFPEQADAYYDGLRH
                     GEVPKLRRITDQDFPFKPGVDVTDVRQVKGLEFDYVVLAEVGETAYPIDDEARHLLHI
                     GATRAAHQLWVLCSGKPSPLLPEDLVSQSL"
     gene            complement(4290..>4655)
                     /locus_tag="VFQ35_14015"
     CDS             complement(4290..>4655)
                     /locus_tag="VFQ35_14015"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931811.1"
                     /translation="EQLNVPFSRLVLGGFSQGGGLATDLALHSPKPPGALAILSGAVV
                     TLGAWRTLMSSRRGLKVFHSHGVQDPVLPFATAEMLRDAMVQAELDHSFVPFRGGHTI
                     PAAVMERFGAFLRGLAAAA"
BASE COUNT          802 a         1534 c         1458 g          861 t
ORIGIN      
        1 gatcttcttc gtcttcctga ctgagttggc gcgtttcggt ccgctctcgg ggcggcatac
       61 gctcgatcac gcgaaccagg cgctgtcgcg taccgtgcgg cgcgtcaggc agatctgcga
      121 tcgcgatccg gccgtggccc cgtgcacttt gacgttgatg gcgaccgatg gtgagacgct
      181 cgtcgcgacc cacggcggca aagagctgtt cgtctcgacc tacaagaagc gttgcgtaga
      241 tcgagatcgc tgcgcgagcc tgtcggccgc ttgtgagggg ccgtcacccg acggacgggt
      301 gaaccacatc atcctatcga gtgagccgtt gcacggagaa aacgtgtggg tgccgctcga
      361 gcccggagaa ctgatcgggg tcggggcgga catgcggccg cgccgtcaac acatcgaacg
      421 ctgcaatcta ccgttggcgg gctgagcctc cgaccgaagc gagccgggac tgagctcgcg
      481 cacacttagc cggcgcaatc caccggcaac ccaaccgtgt cgaacgattc gagcgccgct
      541 tcattcaccc ggagctgact ttgggtcggc tccttcgttc tgctcgctct cactctcact
      601 cgcgctctcg ctctcatcgc cggcctcctc cgattgaccg acgatgagag cgtactcgta
      661 accacgcctg cgctcgggag aaggctcgcc ctcggccaag agaacgttgg tgacccgacg
      721 cacgcctcgg cgctcgacgc gattgggaat tgcccgctct tggcagtgag cctgccccgc
      781 caaaacgagc aaccgccgca tcggcgcgtg ctcgagcacc cagcgcgccg ccgtgtgtgc
      841 catgctttcg tcccacacca cctgggcctg atagtagcga tccaaggtct gctcggtgac
      901 tcccggatga tctttcatcc gccgctcgaa gtccgcgcgg tgctgtgcgt cgtcgagatc
      961 gagagcggga aggctgcgag cctgacgagg gctcagggca tctaccccgc cttctgcgat
     1021 cttgctggta atttcggcgc gcgcgttcag cgccaccagc ggcagacgca agcggcgcgc
     1081 gcgctccaaa atggggctgt agtatgcgaa agggtagccc cagcgctcct cgtattgagt
     1141 gcgttggagc agcgtagtct ggtcgatctt gccgcgctga taatcccaga gtgggcgttg
     1201 ataatcgctg gcccacatct cgaacccaac cccgagctcc aggcccaacg ctggtgcacg
     1261 ctcggccaag cgctcgaata gccagagctc gccgtaatgc tgtgccgctc gggtgtgctc
     1321 ttcgcccgcg caaacgacgt cggctttcag aagctccgtc gcgacttgcg cttcgggcac
     1381 gtcgctaccg tcgctgagcc gcttgaaacg caccggcccg cgcgcgcgct gcaccacgtc
     1441 cggcggtacg cgatgcttcg gcttctctgc gacgaccggc tctcgatcgg gctcgcgcac
     1501 caggtcataa ccgacgttgg aaggtcgcgc cgcgttgcag gcaccagcgt gagctgcgag
     1561 cagcatcagc ggccagaaac gcgccgcacg gaggaggaga gctactcgag tacagtcctg
     1621 aatcaccgag aaaagcgcgc ttcgcatctt cacaggcttt gcgagaccag atcttctggc
     1681 aaaagggggc tgggttttcc ggagcaaagc acccaaagtt ggtgcgccgc gcgcgtcgcc
     1741 ccgatgtgca gcagatggcg cgcctcgtcg tcgattggat acgcggtctc accgacttcg
     1801 gcgagcacga catagtcgaa ctcgaggcct ttcacttgac gcacgtcagt tacatccacg
     1861 ccgggtttga acgggaaatc ttgatcggtg atgcgcctca atttggggac ctcaccgtgc
     1921 cgcagcccgt cgtagtaggc atccgcctgc tcaggaaagc gagtgaccac ggcaatcgag
     1981 gcgttcggtt cctcgctcaa caaatctcgc agcgcatcgg ccaggaacgc caccgaatcg
     2041 cccatatggc cgaatgcgaa cagctcgacg ggggcgccat ggcgcgtggc ctccggcgca
     2101 tcgggactca cgagcggtcc gagcacgtgc cgcgcgaagg cgatgatctc gtgcgtggat
     2161 cggtaactga gcttgagcgg ctccacttcc acgtgcgaga gaccgagctc acccagcact
     2221 tctttccagt tggtgaaacc gttatctagt agcagccgct gctggatgtc gccggccagg
     2281 gtaacgctct cggaacgatt cacggtgttc atgacgaccg cgagctcgac cgggctcaaa
     2341 tcctgcgctt cgtcgatcag gatgtgctcg tagataaccg ggtctttgct cgttgcgcta
     2401 cgggtgaggg ggccccgcag gcgttggctc aagcgcaaga tcagggtgtc gtcttcgggc
     2461 tcgaggcgcg cgcgatcttc gacgtcgcgg ccatcgacac caacgcgcgg cgccggatcg
     2521 gcgctatcgt catcgtcgtc gtcctcggat ttccgagagc gcccccgtcc tttgccttcg
     2581 tcttcctcgt ctcggcctcg ttcttcccgc cgttccgcgc gtcgctcgga cttttcgatg
     2641 agctcgtcca acacttcgct aacgcgcgcc gcgcagtagg tgtgcgcgcg ctccaggtct
     2701 gcgtccgtga aatcgccgcc cgctcgatcc cgatagacct tgcggatcaa ggcccgatcg
     2761 gagagcagct cggcccagtg ttgagccacg tcgcgagccg ccgaaatccc gttcttgatg
     2821 gtgcgctcga ggcttacgcg agcgttggtc gacagctgac gcatctctgc ctcttcgagt
     2881 cgagtcgaga gcgcgtggag acgatgcgcg atgattcgct tccggctacg atcccagccc
     2941 tcgagcaagg tcgcggcggc gcctggcgta cgcgcctctt cggccaatgc ggcgcgcact
     3001 tcgacgtcga gcttggcctc gaagcgcgcg acatgcgctt cgattgcgtg cagcatggca
     3061 gggtgtttct tgagccgcgt gacgaccgcc ggcgcgtcct cgcgatagtg cgaggggaga
     3121 cgaggaaaca gggcctggcg caagcgcgac acgaaatctt cgtaggtgcg gatcgcgacg
     3181 ccgttcacgc cgagcgcggg taagacaccg ttgatgtagc gtgccagcgc cgcgttgaag
     3241 acgatgacca gcatccgatc cggcctaaat cgccttggat cttggaacgc cagataggct
     3301 aggcggtgca acccgatcgt cgttttgccg ctgcccgcac caccctggat cacgaccaag
     3361 cccgagtccg ggcgcgtaat cagctcgaac tgtcgtcggt cgatcagcgg cgtgatctct
     3421 ttgagctgct tgtcttcgcc gcgatctttg ccggtcccga gcttgccggg tcggtggtgt
     3481 tgctcggccc gaatcgcgct gccggcgccg ccggcgagcc gcgtttgagt cgcctctagc
     3541 cgtcgaaagc tcccatctcg cgtgcgcgca aaggagcctt gaggcgtgcc gacccggcgc
     3601 agaaccgcgt cggtgatgac gaggctacga cgtacgttca cggtgccgga cacctcccgc
     3661 ccaccgaact cctcgtcgta ctcttcaccc tcttcgtagc ggtaatacag tcggcttacc
     3721 ggcgcgtcgc gccagtccac gatgcgaatg ttctcacggg tatcgaggtg ggtgccgcgc
     3781 ccaatgagga cttcacgctt gcgctcaccc tcctccagca cgagccgccc gaagtatggg
     3841 gagcgcggat ccacctcgga ttccgtcgtc tcgcgcaagt gcgccgcgag gctctgcagc
     3901 cgttccatct cttgcacgag gggaggaatg tcttccaagc gcgccgacgc gatctgatcg
     3961 cgtaggtcca gcagccgagc atcgtattca gcggagtctc gcgctgcacg cacgcgctgc
     4021 ttgttttggg cgaggtgcga cgtcacccgc tgcaggcagc tttcctcctc ggcaacgatg
     4081 cgatcgcgct cgggatcgtc ggacagggcg gcggagtcag actgagaaac gggcatcacc
     4141 ttcacttgat cttcaaaggc cttccggggt ctttcgaacg cggcggaccg gggcgagtag
     4201 cacgaactga gcccccctcc cagtcatgca agacgggccg aacggtcgat ctgaactgcg
     4261 actacgaccg catctgtgtt cgaacccctt caggccgcgg ccgcgagccc gcgcaggaaa
     4321 gcgccaaacc gctccatcac cgccgcggga atggtgtgcc cgcctcgaaa cggcacgaat
     4381 gagtggtcga gttccgcttg gaccatcgcg tcacgcagca tttccgcggt agcgaagggc
     4441 agcaccgggt cctgcacccc gtggctgtga aacactttta gcccgcgccg gctggacatg
     4501 agggtacgcc aggcgcccag ggtaacaact gcgccgctca ggatcgcgag cgcaccgggc
     4561 ggcttcgggc tgtgcaatgc gagatcagtc gcgagcccgc cgccttgaga aaacccaccg
     4621 agcaccagtc gactaaacgg cacgttcaac tgttc
//
LOCUS       DASTKS010000681         7378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1895061, whole genome shotgun sequence.
ACCESSION   DASTKS010000681 DASTKS010000000
VERSION     DASTKS010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895061"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFQ35_14020"
     CDS             <1..198
                     /locus_tag="VFQ35_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931812.1"
                     /translation="APSLAGLHLVDEGLWRVMERGLEKNLAARWQTADEFAAELAGWS
                     EAHAARARRNTPRRAGPRLVA"
     gene            267..839
                     /locus_tag="VFQ35_14025"
     CDS             267..839
                     /locus_tag="VFQ35_14025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9931813.1"
                     /translation="MIAIGIITGSTRPGRLNEGVANWVLENATRHGGARFELVDIETY
                     ELPLLDEPVPPSLGRYSKPHTKKWAEKIATFDAFVFVTPEYNHGTSGALKNALDFIYA
                     EWNNKAAGFVSYGSVGGTRAVEHLRGVMAELQIADVRQQVMLSLMDDFEEYRTFKPRP
                     HHVKLLETLFEQVISWGSALKTLRSSAAGS"
     gene            1039..1767
                     /locus_tag="VFQ35_14030"
     CDS             1039..1767
                     /locus_tag="VFQ35_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9931814.1"
                     /translation="MVNRDSIHSNELRLREARRKLLVATAASTAGILLASCAASARGG
                     DSPSSQSQGTEAEVTPGEDLMQEHGVIERVLLIYDEAARRIEQGQQLELSVLSGAGLI
                     VRRFVAEYHEKLEEQFVFPPLQKAGRESELVAVLLRQHERGRQLTEEIVKRAQSAASP
                     ELAQALRAFVRMYRPHAAREDTVVFPAFRDVVGGAAYRELGEQFEDKEHQLFGEHGFE
                     NIVKEVSKLEAALKIDNLADFTPP"
     gene            1918..3183
                     /gene="serS"
                     /locus_tag="VFQ35_14035"
     CDS             1918..3183
                     /gene="serS"
                     /locus_tag="VFQ35_14035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9931815.1"
                     /translation="MLDVKQIRENPDAVRRAIADKHVDLNLDELLAAADKVLELSQAQ
                     QSLQEKRNRNAKLIPSAAPDERPTLVAEGRKIGEELAALKPAIDAAEAVLRDWLLKVP
                     NIPAEQAPRGKSEADNVVVRIVGEPKQFDFTALDHVELLEKHGWSDLPRIAKIAGSRS
                     YALKGLAVLLEQSLLRFAIDRLVERGFTPMSVPSFASEEAFIGTGHFPSGRDQVYRIE
                     LDDKYLAGTSEVTLNALHSGEILSEADLPIRYAGISACFRREAGSAGKDVRGLIRVHQ
                     FNKVEQYVICKNDPEESERWHNTLLEVSESILRDLEIPYRVVECCTGDMGLGKYRMFD
                     IESWVPSEKLYRETHSCSNLHEWQARRSNLRYRDSQGKVRFCHTLNNTAIATPRFLVP
                     FLENHQRADGSVHIPAKLRPYLANREVLA"
     gene            complement(3316..4092)
                     /locus_tag="VFQ35_14040"
     CDS             complement(3316..4092)
                     /locus_tag="VFQ35_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931816.1"
                     /translation="MRPELELVSKGTIAVQLTDNTILITGGSSGIGLALAARLVRAGN
                     QVIVTGRRAHLLDEVRKQLPSVVTETSDAASPSERTALVESLTRRFPKLNVLINNAGI
                     QRKVDFLQSEPWEETREEVAINLEGPIHLASLLLPHLLRQPKSAIINVSSGLAFVPLA
                     AVPVYSATKAAIHSYTLSLRYQLRDTPVEVIEVIPPAVHSNLGGKHDFGVPTDEYADS
                     VMAQLAEGRPEITYQFSAATSQASRAELDAMFTRLNAPRT"
     gene            complement(4128..4916)
                     /gene="dapB"
                     /locus_tag="VFQ35_14045"
     CDS             complement(4128..4916)
                     /gene="dapB"
                     /locus_tag="VFQ35_14045"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9931817.1"
                     /translation="MKLAIFGATGRMGLAIIRLLQVDPDVQIVGGACSSNDPAHGRDL
                     GELAGIGSIGVVSTADVGSALLGADVVIDFSTASAVVPLYAVAARQKVAIVSGTTNLD
                     DAAQRALNKAAEAVPVLWSANMSLGIQVLAELVQQAVKKLGPAFDVEIMEIHHRKKID
                     APSGTAKRLAEAAHLARPELKDLHSRAGDVGARTNEELGVFGLRGGDVIGDHTVFLFG
                     SGERLELTHRASNRDLFARGAIAAAKFLPGKKPGRYTIADVLNG"
     gene            complement(4923..5816)
                     /gene="dapA"
                     /locus_tag="VFQ35_14050"
     CDS             complement(4923..5816)
                     /gene="dapA"
                     /locus_tag="VFQ35_14050"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9931818.1"
                     /translation="MTSLPAGAYTALVTPFGPDGSSVDWNAFEALVASQLEGKITGLV
                     PCGTTGESPTLTDSEQRELTRRTVSLAKGRATVVVGTGSNNTKKTIESSKAALEAGAD
                     GVMLVMPYYSKPSQEGLFRHIKAVASAVDCPVMLYNIMGRTGVDLAVDTLLRVLDASP
                     NVVAIKDASGNVSYCQDLLARAEGRILVLSGDDALAVPMMSVGARGVVSVTSNLYPRA
                     VSEIVEDMLANRLADAMRKDKKLLPVHRALFCEPNPQPVKAALALKGRMSDSVRLPLV
                     SASEASKARLRETMAAYDASL"
     gene            complement(5873..7174)
                     /locus_tag="VFQ35_14055"
     CDS             complement(5873..7174)
                     /locus_tag="VFQ35_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9931819.1"
                     /translation="MSKPRQDSVPPSARSVAARVLGRVHNDQAFAAAALDAELSRYAQ
                     LDARERGLATELVYGVLRCEGALDARISRHAPKGIKDRETRLHLLVAAYQLLLLDRVP
                     AFAAVDAAVSLVRKQRGDRVAAFANAVLRKLAASGERLALDAALRESAQPWLWQRLVE
                     SVGEDEALALLGGGDIAPTVGVRLVAGREAPAWAREASTGRVSPRARRIERRGDPKQL
                     EGYADGSFVVQEEGAQAVALLLGARPGERVLDACAGRGQKASLLAEQVGASGEVFATD
                     VHPPKLDALQSEFRRLRLPAPRVAAVDWSAGVGDVAEGFDRVLVDAPCTGTGTLRHRP
                     EIARRLSAEDPARLGALAESILRSASTRARPGGRVVFAVCSVLTAEGEAVVERVRDVL
                     EPVAFDDSEVPGLVPNGATYLRLLPHAHGTDGYFVASFVRR"
     gene            complement(7176..>7378)
                     /locus_tag="VFQ35_14060"
     CDS             complement(7176..>7378)
                     /locus_tag="VFQ35_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9931820.1"
                     /translation="PIPVLDGGHFLFFLIEGALRRPLPLRAREVMHIAGMAVLVGLTL
                     LAFKNDVERRWEGISLAKGAAE"
BASE COUNT         1308 a         2369 c         2451 g         1250 t
ORIGIN      
        1 gccccgtcgc tcgctggcct ccatctcgtc gacgaggggc tgtggcgcgt catggagcgc
       61 gggctcgaga agaacctcgc ggctcgctgg cagaccgccg atgaattcgc ggcggagctc
      121 gcgggttggt cggaggcgca cgccgctcgg gcaaggcgga atacgccacg tcgcgcaggc
      181 ccaaggctcg tcgcgtgaga gtgcaaagcc cgacttgacc caccacgagc ggacccaacg
      241 atcacggctc aacagaggag cgctacgtga tcgcaattgg aatcattacc ggcagcactc
      301 gtcctggtcg gctcaacgaa ggcgtcgcga actgggttct cgagaacgcg actagacacg
      361 gtggggctcg cttcgagctc gtggacatcg aaacctacga actccccttg ctggacgagc
      421 cggtcccgcc atcgctcggc cggtactcga agccccacac gaagaagtgg gccgagaaga
      481 tagcgacctt cgacgcgttc gtcttcgtta ctccggagta caaccacggc acctccggcg
      541 cactcaagaa tgccctcgat ttcatctacg ccgagtggaa caacaaggcc gcgggcttcg
      601 tcagctacgg cagcgtcggc ggcacccgcg ccgtcgagca ccttcgcggc gtcatggcgg
      661 aactccaaat cgccgatgtt cggcagcaag tgatgctgtc gctgatggac gacttcgaag
      721 aataccgcac cttcaaacct cggcctcacc acgtgaaact gctcgagacg ctgttcgagc
      781 aggtcatctc ttgggggagc gcgttgaaga ctctgcgctc gtccgccgcc gggagctgaa
      841 ccttcgcgcc gacgtcgaca gcagccgatt tcgaggcgtg cgctcgcgct gtccgtggct
      901 tttcggactc ctggtcatcc gctcgcgacc gacagtttct tccgagaccc tgaaacgagc
      961 gacaccttgg caataacgtc acacgtaaac gacgcgccaa cgcatttcag agtgcctttc
     1021 cgagtgtatg aagctcgaat ggtcaaccgc gattcgattc acagtaacga actccggctg
     1081 cgcgaggcgc gtcgcaaact tctggtcgcg acggcggcgt ccaccgctgg catcttgctc
     1141 gcgagctgtg ccgcgtccgc gcgcggcggg gattcgcctt cgagtcagag tcaaggcact
     1201 gaagccgagg tgacgcccgg agaagatctg atgcaggagc atggtgtgat cgagcgcgtc
     1261 ttgctcatct acgacgaggc ggcgcggcgc atcgagcaag gccaacaact cgagctgtcg
     1321 gtcttgagcg gcgcgggact catcgtgcgt cgcttcgttg cggagtatca cgagaagctc
     1381 gaagagcaat tcgtcttccc tccgctgcag aaggcgggtc gtgagtcgga gctcgtcgcc
     1441 gtgttgttgc ggcagcacga gcgcgggcga cagctcaccg aggaaatcgt caagcgagcc
     1501 caatctgcgg cctcgccgga gctagctcag gccctgagag ccttcgttcg tatgtatcgg
     1561 cctcacgccg ctcgcgaaga tacggtcgtg ttccccgcgt tccgcgacgt cgttggcggt
     1621 gctgcttatc gcgagctcgg tgagcagttc gaggacaagg agcatcaact cttcggcgag
     1681 cacggcttcg agaacatcgt gaaggaggtc tcgaagctcg aagccgcgct caagatcgat
     1741 aatctcgcgg attttacgcc gccgtgacga gcggtctgcc ccgcgcgccc gacatacggg
     1801 gactttactt cccgagctcc tggccatgtg gccgcagcaa gaacttccgc cggcggcgag
     1861 aaaccgcggg ttcgctgcgg cgaatggcgc tcgtggtgat agcctccgtg ggtcaaaatg
     1921 ttggatgtca aacaaattcg cgaaaacccc gatgcggtgc gacgcgcgat cgcggacaag
     1981 cacgttgatt tgaatttgga cgagttgctc gcggcggcgg acaaggtgct cgagctctca
     2041 caggcccaac agagccttca ggaaaagcgc aaccggaacg ccaagctgat ccccagcgcc
     2101 gcccccgacg agcgcccgac tctcgtcgcc gagggccgca agatcggcga ggagctcgcg
     2161 gccctgaagc ccgcgattga cgcggccgag gccgtgctgc gcgactggct gctcaaggtg
     2221 ccgaacattc ccgccgagca ggcgccgcgc ggaaagtccg aagccgacaa cgtggtggta
     2281 cgcatcgtcg gcgagccaaa gcagttcgat ttcaccgccc tcgatcacgt cgagctcctg
     2341 gagaagcacg gctggagcga cctgccgcgg atcgccaaga tcgcgggctc gcgctcgtat
     2401 gctctcaaag gactcgccgt cctgctcgag caaagcttgc tccggttcgc gatcgatcgc
     2461 ctggtcgagc ggggtttcac tccgatgagc gtcccgtcgt tcgcgagcga ggaggctttc
     2521 atcgggaccg gccacttccc ctccggccgc gaccaagtgt accgcatcga gctcgatgac
     2581 aagtacctcg ccggcacttc cgaggtgact ttgaacgccc tgcacagcgg cgaaatcctg
     2641 agcgaagccg atctaccgat tcgctacgcg ggcatttcgg cgtgcttccg acgtgaagcg
     2701 gggagcgctg gcaaggacgt gcgcggcctc atccgcgtgc accaattcaa caaggtcgag
     2761 cagtacgtga tctgcaaaaa cgacccggag gagtccgagc ggtggcacaa cacactgctc
     2821 gaagtttccg agagcatcct ccgcgatctc gaaatccctt atcgcgtcgt agaatgttgc
     2881 acgggcgaca tgggtctcgg caagtaccgt atgttcgata tcgaatcctg ggtgccgagc
     2941 gagaagcttt accgcgagac tcacagctgc tcgaacctgc acgagtggca agcgcgccgc
     3001 tcgaaccttc gctaccgtga cagccagggc aaagttcgct tttgccacac tctcaacaac
     3061 accgcgatcg cgacgcctcg ctttttggtc cctttcttgg aaaaccatca gcgcgccgac
     3121 ggctccgtcc acatccctgc caagcttcgc ccttatctcg caaaccgcga agtgctcgcc
     3181 tagcgcctca cgccgtcgca gcttacgcgc cgagctcggc accgcccgac cgaaccgaca
     3241 aaggagcgct ctcgacgagc gctcttggtt cggtgcgagt cgggcgattc ggaagggcga
     3301 accgggcgct cgggatcagg ttcggggcgc gttcagccgc gtgaacatcg cgtcgagctc
     3361 ggcgcgggag gcttggctgg tggcggccga gaactgatac gtaatctcgg gccgcccctc
     3421 cgcgagctgc gccatcaccg agtcggcgta ctcgtcggtg gggaccccga agtcgtgttt
     3481 tcccccgagg ttcgaatgca cggcgggcgg aatcacctcg atgacttcga cgggagtgtc
     3541 gcgcagctga taccgcagcg aaagcgtata cgaatgaatc gccgccttcg tcgcggagta
     3601 cacgggtacg gcggcgagcg ggacgaacgc aagcccggag ctcacgttga tgatcgccga
     3661 cttgggctga cgcagcaggt gcggcagcaa cagcgacgcg aggtggatcg gcccttcgag
     3721 gttgatggcg acttcttcgc gggtctcttc ccacggctcg ctctgcagga aatcgacttt
     3781 gcgctggatg cccgcgttgt tgatgaggac gttgagcttc ggaaagcggc gcgtgagcga
     3841 ctccacgagc gccgtgcgct cggaggggct ggcggcgtcg ctcgtctccg tgacgaccga
     3901 gggcagttgc ttccgcacct cgtcgagtag gtgagcgcgg cgtcccgtga cgatgacctg
     3961 attgcctgca cgcacgaggc gcgccgccaa cgcgaggcca atgcccgagg agcctccggt
     4021 gatcaggatg gtgttgtcgg tgagctgcac ggcgatggtt cctttcgaga cgagctcgag
     4081 ctctgggcgc acgtttcgac gttacgagct ctgctagcgc acggcgctca gccgttgagc
     4141 acgtcggcga tggtgtagcg ccccggcttt tttccgggga ggaatttggc ggcagcgatc
     4201 gcgccgcgcg cgaacagatc gcggttcgac gcgcggtggg tgagctcgag gcgctcgcca
     4261 gaaccgaaga ggaacacggt gtggtcgccg atcacgtcgc cgccgcggag gccgaagacg
     4321 ccgagctctt cgttggtgcg tgcgccgacg tcaccggcgc gcgagtggag atctttgagc
     4381 tcggggcggg ccaggtgggc cgcctcggcg aggcgcttcg cggtgccgct cggcgcgtcg
     4441 atcttctttc ggtggtggat ctccatgatc tccacgtcga acgcgggccc gagcttcttc
     4501 accgcctgtt ggacgagttc ggcgagcacc tggatgccaa ggctcatgtt cgcggaccag
     4561 aggacgggca ccgcttctgc ggccttgttg agggcgcgct gcgcggcatc gtcgaggttc
     4621 gtggttccgc tgacgatcgc caccttctgg cgtgcggcga ccgcgtagag cgggacgacc
     4681 gcgctcgcgg tggagaagtc gatgacgacg tccgcgccga gcaatgccga gccgacgtct
     4741 gctgtcgaga cgacgccaat cgagccgatg ccggcgagct caccgagatc gcgcccgtgt
     4801 gcggggtcgt tcgacgaaca ggcgccgccg acgatctgca cgtcgggatc gacctggaga
     4861 agtcgaatga tggcgaggcc catgcgccca gtggcgccga aaatcgcgag tttcatgtga
     4921 gcctagagcg atgcgtcgta agcagccatg gtttcgcgca agcgagcttt ggaagcttcg
     4981 ctcgcggaga cgagcgggag gcgcaccgag tcactcatgc gacctttgag cgcgagcgcc
     5041 gccttgacgg gctgaggatt cggttcgcag aagagggcgc gatgcacggg caagagcttc
     5101 ttgtccttgc gcatcgcgtc ggcgaggcgg ttcgccagca tgtcttcgac gatttcgctg
     5161 accgcgcgtg ggtagaggtt gctcgtgacg ctcacgacgc cgcgcgcccc gacgctcatc
     5221 atcgggaccg cgagcgcatc gtcgccggag aggacgagaa tgcgtccttc ggcgcgcgcg
     5281 agcagatctt ggcagtagct gacgttgccg ctcgcgtcct tgatcgcgac gacgttgggc
     5341 gaggcgtcga gcacccggag cagagtgtcg acggcgagat cgacgccggt gcggcccatg
     5401 atgttgtaga gcatcacggg gcagtcgacc gcgctcgcga cggccttgat gtggcgaaac
     5461 agcccttctt gcgagggctt gctgtagtac ggcatcacga gcatgacgcc gtcggcccct
     5521 gcctcgagcg ctgccttgga gctctcgatg gtcttcttgg tgttgttgga gccagtgccg
     5581 acgacgaccg tagctcgacc cttggcgagg ctcaccgtgc ggcgcgtgag ctcacgctgc
     5641 tcgctgtccg tcaacgtcgg gctctcaccg gtggttccgc aggggaccag gccagtgatc
     5701 tttccctcca gttgcgacgc gacgagtgcc tcgaacgcgt tccaatcgac cgatgaccca
     5761 tcgggaccga acggagtgac gagcgccgtg tacgcgcccg cggggagcga tgtcatggcg
     5821 atgctctgcc gcgtttctcc ccgaaaaaca agtcggagtg gcgcaaaacc ggctagcgcc
     5881 gcacgaaact cgccacaaag tagccgtcgg tgccgtgcgc gtgcggtaaa agccgcaggt
     5941 atgtcgcgcc gttcggcacg aggccgggaa cctcgctgtc gtcgaaggcg acgggttcga
     6001 gtacgtctcg cacccgttcg acgacggctt caccctcggc ggtgagcaca ctgcaaaccg
     6061 cgaagacgac gcggcccccg gggcgagctc gagtcgacgc cgagcgcaag atgctttcgg
     6121 cgagggcgcc gaggcgagcc ggatcttcag ccgagaggcg tcgagcaatt tcggggcgat
     6181 gacgcaatgt gccggtgcca gtgcacgggg cgtcgaccaa aacgcgatcg aaaccttccg
     6241 ccacgtcacc cacccccgcg ctccaatcga cggcggctac gcgcggcgct ggcaaacgca
     6301 gtcgtcgaaa ctcgctttgc aaagcgtcga gcttcggggg atgcacgtcg gttgcaaaaa
     6361 cctcgcccga ggctccgact tgttcggcga gcagcgaagc tttctgcccg cggcccgcgc
     6421 aggcgtcgag cacgcgctca ccggggcgcg caccgagcaa taacgcgacc gcctgggcgc
     6481 cttcttcctg aactacgaag ctaccatcgg cgtagccctc gagctgcttc ggatctcctc
     6541 gtcgctcgat gcgtcgcgcg cgcggcgaca cgcggccggt ggacgcttca cgagcccaag
     6601 ccggcgcttc gcggcccgcg accaggcgca ctcccaccgt aggagcgatg tcgccgccgc
     6661 cgagcaacgc cagcgcttcg tcttcgccga ccgactcgac gaggcgctgc cagagccagg
     6721 gctgcgcgct ttcgcgaagc gccgcgtcga gcgcgagccg ctcgccactc gcggcgagct
     6781 tgcgcagtac cgcgttggcg aacgcagcca cccgatcgcc ccgttgcttg cgcacgagcg
     6841 agacggcggc gtccactgcg gcgaaggcgg gcacgcgatc gaggagcagc agctgatacg
     6901 cggcgaccaa gagatggagc cgtgtttcgc ggtctttgat gccttttggc gcgtgccgcg
     6961 aaatccgcgc gtcgagcgca ccttcgcagc gcaacacgcc gtagacgagc tcggtcgcga
     7021 gcccgcgctc ccgggcgtcg agttgcgcgt agcgcgagag ctcggcgtcg agggcggcgg
     7081 cggcaaacgc ctggtcgttg tggacgcggc cgagtacgcg cgcggcgacg ctgcgcgccg
     7141 agggcggcac agaatcctga cggggcttgc tcactttatt ctgcagcgcc tttcgcgagc
     7201 gagatccctt cccaacggcg ctcgacgtcg ttcttgaacg cgagcagcgt gaggccgacc
     7261 agcaccgcca tgcccgcgat gtgcatgacc tcacgcgcgc ggagcggcag cggccgacgc
     7321 agcgcgccct cgatcaagaa gaacaagaag tgaccgccgt cgagcacggg gatgggga
//
LOCUS       DASTKS010000682         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1671072, whole genome shotgun sequence.
ACCESSION   DASTKS010000682 DASTKS010000000
VERSION     DASTKS010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671072"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VFQ35_14065"
     CDS             <1..1629
                     /locus_tag="VFQ35_14065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET9931821.1"
                     /translation="QAKQTLQAIREGSVDALVVEGPSGPAIYTLEGPDEPFRTFVEGM
                     QEGAITLSAEGELLYANSFFAELVGCVPSEVIGRPLIEFVAPDYRYTCRSLLEQGLSR
                     PIKGNLRLLRGNERISVQLTLSPLNRRETPTCCGVVFDLREREQADAAATARDAAEQA
                     NAAKDKFLAVLSHELRSPLNAVLGWAQILGRNPALDDASRRAAQAIERNARVQAQLIA
                     DLLDISRIVMHKLHLEMEVADFKGIVESAVQAARLGLSATGKKLVIESSSPDAEVYVY
                     GDPARLQQVVTNLLNNAMNFTESGGRIDVTLSLEGEGAVLTVRDTGVGIAPTQIEHIF
                     DPFHQASEDVNHRKGGLGLGLAIARQIAGAHGGRIDAASEGHGKGSTFTLQLGPVVTP
                     AFVVRTVSSDERELSKVQVLLVDDESDTLDVTRYLLEGAGASVQTADSAAAALEVIQT
                     QQFDVVVSDIGLPVQDGFALMREVRARGFGADALPAIALTGYASSSDARRSSAAGFQM
                     HMAKPVDAQALVRAIKTLSAKKLTERGSVSLDGT"
     gene            1892..2188
                     /locus_tag="VFQ35_14070"
     CDS             1892..2188
                     /locus_tag="VFQ35_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931822.1"
                     /translation="MANGNNGSDLSLERPPKSRRPKQNGSAPSKGGPASSRKGAAPSG
                     KVQAAPSSTGATHDAEGVMKLGKGTPVADARVNGNGHAVDGLRKPRERGLNGFG"
     gene            2237..>3566
                     /locus_tag="VFQ35_14075"
     CDS             2237..>3566
                     /locus_tag="VFQ35_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9931823.1"
                     /translation="MVEVLKAMKQGDFSVRFEYEQHGALSRVGELLNDVIVLSEHTTA
                     ELVRIGKVVGQDGRMHERASIGTAKGAWAQGMHSVNQLIADLVAPTNEVARVITAVAR
                     GDLSQKMVLEIEGRPVRGEFLRIGTTVNAMVDQLNSFAAEVTRVAKEVGSEGKLGGQA
                     EVKGVSGTWKDLTDNVNGLAANLTAQVRSIAKVTTAVANGDLSQKITVDARGEIYELK
                     NTINTMVDTLRSFAAEVTRVAKEVGTEGKLGGQADVKGVAGTWKDLTDNVNAMASNLT
                     VQLRDVSKVATAIASGDLTRKITVDARGEILQIKDVINMMVDQLNSFAAEVTRVAKEV
                     GTEGKLGGQADVKGVYGTWKNLTDNVNAMASNLTVQLRDVSKVATAIANGDLTQKITV
                     DVKGEILQIKDVINKMVDQLNSFAAEVTRVAKEVGTEGRLGGQAEVRGVSG"
BASE COUNT          703 a         1073 c         1221 g          569 t
ORIGIN      
        1 caggcgaagc agacgcttca ggcgatccgg gaaggctcgg ttgacgccct cgtggtcgaa
       61 ggcccgagcg gcccggcgat ttacaccctc gaaggacccg acgagccgtt tcgcacgttc
      121 gtcgagggca tgcaggaagg cgcgattacc ctgtccgcgg agggggagct cctctatgcg
      181 aacagcttct ttgcagagct cgtcggctgc gttccgtcgg aggtaatcgg tcggcccctc
      241 atcgagttcg tcgcgccgga ctaccgctat acgtgtcggt cgctactcga gcagggcctg
      301 tcacggccca tcaagggcaa cctgcgcttg ctgcgcggaa acgagcggat ctcggtgcag
      361 ctcaccctga gcccgctgaa ccgacgcgaa acaccgacgt gctgcggtgt cgtcttcgat
      421 ctgcgcgagc gtgagcaggc agacgccgcg gccacggccc gtgacgcggc cgagcaagct
      481 aacgctgcca aggacaagtt cctcgccgtg ctgagccacg agttgcgttc acctttgaac
      541 gcggtgctcg gctgggcgca aattttggga cggaaccccg cgctcgacga cgcgagccgg
      601 cgcgccgccc aagccatcga gcgcaacgcg cgagttcagg cgcagctcat cgccgacttg
      661 ctcgatatct cgcgcatcgt gatgcacaag cttcacctcg aaatggaggt cgcggacttc
      721 aagggcatcg tcgagtccgc cgtgcaagca gcgcggctcg gtctgagcgc cacgggcaag
      781 aagctcgtga tcgagtcgag ctcgcccgac gccgaggtgt acgtctatgg ggacccggcg
      841 cgtttgcagc aagtcgtgac caacttgctc aacaacgcca tgaacttcac ggagagcggg
      901 ggccgcatcg acgtgactct gagcctcgaa ggcgaaggcg cggtcctcac cgtgagggat
      961 actggcgtcg gcatcgcgcc cactcaaatc gagcacatct tcgatccgtt ccatcaagcc
     1021 tccgaagacg taaatcatcg caagggcggg ctcgggctcg ggctcgcgat cgcgcggcaa
     1081 attgcggggg cgcacggcgg gaggatcgac gctgccagtg aaggtcacgg gaagggcagc
     1141 acctttacgt tgcagctcgg gccggtggtg acgcccgcct tcgtggtgcg gaccgtgagc
     1201 tcggacgagc gcgagctttc gaaagtgcag gtgctgctcg tggacgacga gagcgacacg
     1261 ctcgacgtga cgcgctacct tttggaaggc gctggcgcgt cggtccaaac tgccgacagc
     1321 gcggccgcgg ccctggaggt catccagacc cagcagttcg acgtcgtggt gagcgacatc
     1381 gggctcccgg tacaagacgg cttcgcgctg atgcgcgagg tgcgcgcgcg gggcttcggg
     1441 gcggacgcgc tgcccgcgat cgctctgacc gggtacgcga gctcttccga tgcacgtcgc
     1501 tccagcgcgg ccgggtttca gatgcacatg gcgaagcccg tcgatgcgca ggcgctcgta
     1561 cgcgccatca agaccttgtc cgcaaaaaag ctgacagagc gcggaagcgt gtccctcgac
     1621 gggacgtgag cgaccggctg tgagggctca tcagcgtggc ctcggatcgc tagcgtgcga
     1681 acgctcgctt cactcggaag ggtcgcgttc gagtgcacgc cctgcggtga acaaattcgc
     1741 ctcgctttgc caagccgcca catggctcga atgcggcggc gaatcgggtc gagaagcacg
     1801 gagcctcttg atttgagagc agcttcgccg aactctaaag ttcccctttt cgcgcgccga
     1861 gcgcgcccgc gtccccccgg aggattttgc gatggccaat ggaaacaatg gctcggatct
     1921 atctctagag cgtccgccga aatctcgtcg acccaagcaa aacggcagcg cgccttcgaa
     1981 gggtggtccg gcttcgtcgc gtaagggagc cgcaccgagc ggcaaagtcc aagcggctcc
     2041 gtcttccacc ggcgcgacgc acgacgcgga aggcgtgatg aagctcggca aggggacgcc
     2101 ggttgcagac gcgcgcgtca atggaaacgg ccacgcggta gatggcctgc ggaaacctcg
     2161 cgagcgcggc ctcaacggct ttggatagct ttccgcgtac gagcgcgtcg agtcgagccg
     2221 ctccgagatc gagcacatgg tcgaagtcct gaaggccatg aagcagggcg atttctcggt
     2281 gcgcttcgag tacgagcagc acggcgcgct cagtcgcgtg ggtgagctgt tgaacgacgt
     2341 catcgtgctc agcgagcaca cgacggcgga gctcgtgcgc atcggcaagg tcgtcggtca
     2401 ggacggccgc atgcacgagc gcgcttcgat cgggacggcc aagggcgcat gggcgcaggg
     2461 catgcactcg gtgaaccagc tgatcgccga cttggtggcg ccgaccaacg aagtcgcgcg
     2521 cgtgatcacg gcggtggcgc gcggcgattt gtcgcagaaa atggtgctcg aaatcgaggg
     2581 ccgcccggtg cgcggcgagt tcctccgcat cggtaccacc gtgaacgcga tggtggatca
     2641 gctgaactct ttcgctgcag aagtgacccg cgtggccaag gaagtgggta gcgaaggcaa
     2701 gctcggcggt caggccgaag tgaagggcgt gtccgggacc tggaaggatc tgaccgacaa
     2761 cgtgaacggc ctcgccgcga acttgacggc gcaagtgcgc agcatcgcga aggtgacgac
     2821 ggcggtcgcg aacggcgatc tctcgcagaa gatcacggtc gacgcgcgcg gagaaatcta
     2881 cgagctcaag aacacgatca acacgatggt ggacacgttg cgctcgttcg cagcggaagt
     2941 gacgcgcgtc gcgaaggaag tgggcaccga gggcaaactc ggcggccagg ccgacgtgaa
     3001 gggcgttgcc ggcacctgga aagacctcac cgacaacgtg aacgcgatgg cttcgaacct
     3061 gacggttcag ctgcgtgacg tgtcgaaggt ggcgaccgcg atcgcgagcg gcgacctcac
     3121 tcggaaaatc acggtcgatg ctcgaggaga aattctccag atcaaagacg tgatcaacat
     3181 gatggtcgat cagctcaact cgttcgcggc ggaggtgacg cgcgtcgcga aggaagtggg
     3241 caccgagggc aaactgggtg gtcaggccga cgtcaagggc gtgtacggca cctggaagaa
     3301 cctcaccgac aacgtgaacg cgatggcttc gaacctgacg gttcagctcc gcgacgtctc
     3361 gaaggtggcg accgcgatcg cgaacggcga cttgacccag aagatcacgg tcgatgtgaa
     3421 gggcgagatt ctccagatca aagacgttat caacaaaatg gtggaccagc tgaactcgtt
     3481 cgcggcggag gtaacgcgcg tcgcgaagga ggtgggcaca gaaggtcgcc tcggtggtca
     3541 ggcagaagtg cgaggagttt ccggta
//
LOCUS       DASTKS010000683         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_555623, whole genome shotgun sequence.
ACCESSION   DASTKS010000683 DASTKS010000000
VERSION     DASTKS010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555623"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFQ35_14080"
     CDS             complement(<1..657)
                     /locus_tag="VFQ35_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931824.1"
                     /translation="MISWSRLALLVLLLFAWPACSGDDTPGPGGAGGLAAGGQSGNGS
                     GGTGAAATGGTGNASGGDAEEGGSSSSSGGAHLNAPPAWRCIEAAYGDGRCDCGCGAP
                     DIDCAEQNLAQCEVCNNSGSCSAGACPGRIDVSDVSHCLAVPNAWRCERQRFGDGVCD
                     CGCQTRDVDCPDANASSCESCSLTGSCARAECPATILENDNAHCAVPARWSCAPETYG
                     D"
     gene            complement(837..1223)
                     /locus_tag="VFQ35_14085"
     CDS             complement(837..1223)
                     /locus_tag="VFQ35_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931825.1"
                     /translation="MHSPQIRTTESVSRLPLALPEELWASLSPAQRAVYRRAEALLDA
                     FSFERKISLSRLLSQGPHAEVLRALQVLGSMSLVEVEEEDDEANVTLRALPEEHVRVT
                     GPDGRPRWVFVARPIEAPQVDRSLLN"
     gene            complement(1501..>2122)
                     /gene="secF"
                     /locus_tag="VFQ35_14090"
     CDS             complement(1501..>2122)
                     /gene="secF"
                     /locus_tag="VFQ35_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9931826.1"
                     /translation="ALGAKAPDQPLRSEWIGPRAGAQLRDSAIKSIAISIVFIMAYVA
                     FRFDLRFAPGGVLALIHDALGMVGILVLIGEEINLTTIASALTIVGYSVNDTVIVYDR
                     VRENLGKLRGASFRHLINVSTSEMLGRTLLTNATVQISLLAFFVWGTGTLKHFALSLT
                     IGMLLGAYSSIYVALPLTEWLDRVLFQRLGSSGKGASVGKNATAAV"
BASE COUNT          383 a          675 c          712 g          352 t
ORIGIN      
        1 atcaccgtaa gtctcgggag cacaagacca acgagccggc accgcgcagt gtgcgttgtc
       61 gttctcgagg atggtcgcgg ggcactcggc acgcgcacat gagcccgtga gcgagcagct
      121 ctcgcagcta ctcgcgttgg cgtcggggca gtcgacgtct cgcgtttggc agccgcagtc
      181 gcagacgccg tcgccgaagc gctggcgttc acacctccaa gcgttcggta ccgccaagca
      241 atgcgatacg tcgctaacgt cgatgcgacc agggcaggct cccgcgctgc agcttcccga
      301 gttgttgcag acctcgcatt gcgcgaggtt ctgttcggcg cagtctatgt ctggagcgcc
      361 gcaaccgcag tcgcagcgcc catcgccgta agcggcttcg atgcagcgcc acgcgggagg
      421 cgcattcaag tgagcgccac ccgagcttga gctactgccg ccttcctccg cgtcacctcc
      481 gcttgcgttg ccggttccgc cggtcgcggc agcgccggtc ccgccgctgc cgttcccgct
      541 ctgcccgccc gcggccaatc cgccggcgcc gccgggaccc ggcgtgtcat cgcccgaaca
      601 cgcaggccat gcgaaaagga gcaagaccag caaggccagt cgcgaccacg agatcatcct
      661 acggggttga tgcccggagc tcgcggccgt cattcgccca atctagtcga aacgggcaga
      721 cgcggcgcgg gtacgcccga actaggtgct tgccgcctgg caggcgagac tacgacgcag
      781 cgcgctgcgg ttgcgtcgta gtttcagcgg acgcgctgag tttagcggag tgacactcag
      841 ttcagaaggg agcggtcgac ctgaggtgcc tcgatcggac gggccacgaa cacccagcga
      901 ggccgcccgt caggtcccgt gactcgcacg tgctcctcgg gcagggcacg aagagtgacg
      961 ttcgcttcat cgtcttcctc ttcgacctcg accagggaca tggagccgag cacctggagt
     1021 gcacgaagga cttccgcgtg aggcccctgg ctgaggaggc gggaaagaga aatcttacgc
     1081 tcgaagctga acgcatcgag tagcgcttcg gctcgtcgat acactgcgcg ctgcgctgga
     1141 ctcaagctcg cccacaactc ttccggcaat gcgagcggca aacgggacac gctctcggtc
     1201 gttcggattt gaggcgagtg catgcttttg aacgtaaaca cgtggttcga agtgtcaaca
     1261 gactaggccg gtcagcgggc cgttaaagcg ttcggcagta aatgtgcgcg accagcttaa
     1321 cggcgcgatg ttgcaggcac tgttcggggc cgcgcctgcg cgaacacgcg ggtgagcgcg
     1381 ctccggaaat tgtgaagagc gccgcaggca cggcgcccgg gccgcgcccc cggcggctcg
     1441 agggtacggt gacgtcggtg gagggtacgg tgaggtgggc cggaactgcg tcgccggtgc
     1501 tcagaccgcc gccgtggcgt tcttccctac gctcgcacct ttgccgctcg agccgagtcg
     1561 ctggaagagc actcgatcga gccactcggt cagcggaagc gcgacgtaaa tcgaggagta
     1621 ggcgccgagg agcatgccga tcgtgagcga cagggcgaag tgcttcagcg tgcccgttcc
     1681 ccacacgaag aacgcgagca agctgatctg caccgtagcg ttcgtgagca gtgtacgtcc
     1741 gagcatttcg gacgtgctca cgttgatgag gtgtcggaac gacgcgccac gtagcttgcc
     1801 caagttctca cgcacgcggt cgtagacgat cacggtgtcg ttcaccgagt agccgacgat
     1861 ggtgagcgcc gaggcgatgg tggtgagatt gatctcctcg ccgatcaaca ccaggatgcc
     1921 gaccatgccg agcgcgtcgt gaatgagcgc gaggacgccg ccgggagcga agcgtaggtc
     1981 gaagcggaac gcgacgtacg ccatgatgaa gacgatggag atcgcgatgc tcttgatcgc
     2041 gctgtcccgc agctgagctc ctgcgcgcgg cccgatccac tcggaacgca gcggctggtc
     2101 gggagctttc gcgccgagcg ct
//
LOCUS       DASTKS010000684         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1895953, whole genome shotgun sequence.
ACCESSION   DASTKS010000684 DASTKS010000000
VERSION     DASTKS010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895953"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..330)
                     /locus_tag="VFQ35_14095"
     CDS             complement(7..330)
                     /locus_tag="VFQ35_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931827.1"
                     /translation="MDPALEALWKQTLDAWDEDRAHGAFLEYCRSTQRLAEAAARYRG
                     MTSDRERAEVAQQKLKAVVLLAMSQLESARSERKTPTRPASYLLVVFFLAASLGLLAY
                     VASTR"
     gene            complement(367..798)
                     /locus_tag="VFQ35_14100"
     CDS             complement(367..798)
                     /locus_tag="VFQ35_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931828.1"
                     /translation="MSDAIDARSFESRYRELVADFAKERGNTACVECERCQGCSNSTF
                     CKDSERLLRCHFCTRCQGCTDSSHCRGSKGLIGCQHCIDCENCSSSSYLVRSVGLSGC
                     SYCFGCVGLSNKDFHILNQPYPRKEYFAITARLIKELRIGS"
     gene            883..1542
                     /locus_tag="VFQ35_14105"
     CDS             883..1542
                     /locus_tag="VFQ35_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931829.1"
                     /translation="MSSSFPTRALPALLVIACASQPPPPKVAPGPAPQPSSNGRVTAA
                     SQSLVDGGWGERLLRRLALGVQLPDAAHWREVGGSRWVRLEHPTSRSLLELSTSRERR
                     LVSPEECEQRARLERPELPRPAEGEALDRRRAKLPDETFTNVTVSLEEAGGEELVGHL
                     TFFGASVGRCLVGHFSTRVSGAERDQEVARRLRLAVDGILPTLRRMAADERVQPQPFE
                     R"
     gene            complement(1561..2787)
                     /locus_tag="VFQ35_14110"
     CDS             complement(1561..2787)
                     /locus_tag="VFQ35_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HET9931830.1"
                     /translation="MHAAPKPRDLILALLRRVVLAFAVAILAVTPLEALADSAGQNVR
                     TGSADTGQADAALVRESPGDAPRVGVGRLNATPPPPGFNVFDGGWIRFYYHPSSREHV
                     QALIAESTVIRRELSERLGTQVLPRVRVDVARTPGEMATFAPPEAPYPEYAAGVAYPE
                     LGLVLLSLSPVHPTSEHNVVQVFRHELAHVALFDAASGRHVPRWFNEGFAVFASGESS
                     FERLRTLWMASVADTLIPLNQLDATFPSNEGEAEIAYAEAVDLVRYLVRKREHFRFQS
                     LVERVRGGEDFASALKNAYGVDLPLIEQEWREDVARRYTFWPVLLSGTFVWVGVMGLF
                     VVGWRKKKKRERLVLSRWAEEEAREDAMASELVARSPRVHIVLARGPQQGLPQMPPPE
                     SDVPKVQHEGQWHTLH"
BASE COUNT          551 a         1054 c          952 g          473 t
ORIGIN      
        1 cccccgtcaa cgcgtacttg cgacgtaggc caaaaggccg agacttgcgg ccaaaaagaa
       61 gaccacgagc agatagcttg ccggccgcgt cggggttttg cgctcgctgc gcgcgctctc
      121 gagctggctc atcgccaaga gaaccaccgc cttcaacttc tgctgagcga cctccgcccg
      181 ctcgcggtcc gaggtcatgc ctcggtaacg cgccgccgcc tccgcgagtc gctgggtcga
      241 gcggcagtac tcgaggaatg cgccgtgggc gcggtcttcg tcccacgcat ccagcgtctg
      301 cttccagagc gcctcgagag cggggtccat cggcgtcatc acaaacgcgt gccaggacgg
      361 ccgcgctcac gagccaatgc ggagttcctt gatcaaacgc gccgtaatcg cgaagtactc
      421 cttgcgcggg tagggctggt tcaaaatgtg aaagtccttg ttgctgagcc ccacgcagcc
      481 gaagcagtac gaacagcccg acagcccgac cgagcgcacc aggtaagaac tcgacgagca
      541 gttctcgcaa tcgatgcagt gctggcagcc gattaggccc ttgctgcctc gacaatgcga
      601 gctgtcggta cagccctgac agcgcgtgca gaaatggcag cgcaggagcc gctccgagtc
      661 tttgcagaac gtcgagttgc tgcagccctg gcaacgctcg cactcgacgc acgcggtgtt
      721 cccccgctct ttggcgaagt cggcgaccag ttcgcgataa cgagactcga agctccgggc
      781 gtcgatggcg tcgctcaccg cagcactatt tcatcccccg gccgggcgtt gcaagcacac
      841 gatttgaggc ccctccggcg cgcggtatac aggctttcgc gcatgagctc ctcgtttcca
      901 acgcgcgcgc tgcccgccct tctcgtcatc gcgtgcgcgt cgcagcctcc tccgccaaaa
      961 gtcgcgcccg gacccgctcc gcagccgtcg tccaacggca gagtcacggc cgcgagccaa
     1021 tcgctcgtgg acggcggctg gggcgagcga ctcttgcgcc gcctcgcgct cggcgttcag
     1081 ttgccggacg ccgcgcattg gagggaagtc ggcggaagcc gctgggtccg cctcgaacat
     1141 ccgacgagtc ggtcgttgct cgagctctcg acgagccgcg agcggcgatt agtgagcccc
     1201 gaagagtgtg agcagcgcgc gcgcctcgag cgcccggagc tgccacgacc cgcagagggc
     1261 gaagcgctcg atcggcgccg cgcaaagctc cccgatgaaa ccttcacgaa cgtcacggtg
     1321 agcctcgagg aagcgggcgg cgaggagctc gtcggccacc tcacattttt cggcgcgagc
     1381 gtcggtcgct gcctggtcgg gcacttttcc acccgcgtga gcggtgccga gcgagaccaa
     1441 gaagtcgcga ggcgcttgcg cctagccgtc gacggaattc tgccgacgct caggcgaatg
     1501 gccgccgacg agcgggtcca accgcagccg ttcgaacgct agaacgacga aacgcgagcc
     1561 tcagtggagc gtgtgccact gaccttcgtg ctgcaccttg ggcacgtcgc tttcgggcgg
     1621 aggcatttga ggaagccctt gctgcggccc tcgagcgagc acgatgtgca cgcgcggcga
     1681 acgcgcgacg agctccgacg ccatcgcgtc ctcgcgcgcc tcttcttctg cccagcgcga
     1741 caacaccagc cgctcgcgct tcttcttctt gcgccaaccg acgacgaaca agcccatcac
     1801 gccgacccaa acgaaggtcc cgctgagcag caccggccag aacgtgtagc gacgcgcgac
     1861 gtcctcacgc cactcctgct cgatcaacgg taagtccacg ccgtacgcgt ttttgagtgc
     1921 ggaagcgaag tcttcaccgc cccgcacccg ctcgaccaag ctttggaagc ggaagtgctc
     1981 tcgcttgcgc accaagtagc gcaccagatc gaccgcctct gcgtacgcga tctcggcttc
     2041 gccctcgttc gaagggaacg tcgcgtcgag ctggttcagc gggatcagcg tgtcggccac
     2101 ggaggccatc caaagcgtgc gaaggcgctc gaacgagctc tcgcccgagg cgaacaccgc
     2161 gaacccctcg ttgaaccaac gcggcacgtg tcgccccgac gcggcgtcga acaacgcgac
     2221 gtgcgcgagc tcgtggcgaa acacctggac cacgttgtgc tcgctcgtcg gatggaccgg
     2281 agagagcgac agcaagacga ggccgagctc tggatacgcg acgccggccg cgtactccgg
     2341 atagggagcc tcgggcggcg caaacgtcgc catttctccg ggcgtgcgcg caacgtcgac
     2401 ccgaacgcgc ggcaagacct gagtcccgag ccgctcgctc agctcgcgcc ggatcacggt
     2461 cgactcggcg atcagcgcct gcacgtgttc gcgactcgac ggatgatagt agaaacgtat
     2521 ccaaccgccg tcgaacacat tgaagccggg aggaggaggc gtcgcgttca gacggcctac
     2581 gcctacgcgt ggcgcatcac ctggagactc gcgcaccaac gccgcgtccg cttgtccggt
     2641 atccgcgctg ccggtgcgaa cgttctgtcc ggccgagtca gccaaggcct cgagcggcgt
     2701 cacggccagg atggcaaccg cgaacgccaa gaccactcgc cggagtaacg cgaggatcag
     2761 gtccctcggc ttcggcgctg cgtgcatcac cccaaatatg gaccgatttc gtgccgattg
     2821 ctgccgtcca aaccggcggg ttgcgcaagt cgccggcgac gcttaacgct cggcaaactg
     2881 acccgctggc cgacacgaac cggtgagggt gggacgacga cgcaccccca accgcgaact
     2941 agggggtgtc ccaagttccc cgccctgctg cgagccaaga tcctgcggct gcgaagcagg
     3001 gcctcgtcgc tccgctcctc gaaaggcttc
//
LOCUS       DASTKS010000685         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1671830, whole genome shotgun sequence.
ACCESSION   DASTKS010000685 DASTKS010000000
VERSION     DASTKS010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671830"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="folK"
                     /locus_tag="VFQ35_14115"
     CDS             complement(<1..441)
                     /gene="folK"
                     /locus_tag="VFQ35_14115"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9931831.1"
                     /translation="MTEPGFVNDASPSQPIDVVVGLGSNLGDRIETLRRAVRALAEFA
                     EVRAVSYTYETPPLGPPQPDFMNAAVRLHARLSPTELLARLLEIERAEGRERRVRWGP
                     RTLDLDILWIFGRSVAEPHLVVPHAELKKRAFALLPLLDVAPEAV"
     gene            887..3253
                     /locus_tag="VFQ35_14120"
     CDS             887..3253
                     /locus_tag="VFQ35_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931832.1"
                     /translation="MASEPAPGSDSAGGAPSEALIFVSDASAEAERLTATLRARGYLT
                     VDVPLGLLVGRVAVQRPSLVLCDADAPGALEAVERMREATGGRSVDVLFLGEAGRTLD
                     DQAERVAREGSGIFVRPVDVYALMRKVEAIIGPSSGRLGRPSTVPHANRVPVLVAATR
                     RPYRYDTKARARNFPASTAPTVATVPPPTARPTDVSMARVPSDPAGPDFAAVAQGGLS
                     ADLAALLGRAEQRVLGSRQFGGAGERLSPEAELEAVLPEDLLAALDEPLDELDDDDDD
                     EDGTPGTHGSDSNLGTRTGSRPGQDSTAGTSPGGSVNPFAQRDANTTSGGDREPAAPE
                     PAPPTPPAHPGRTAPPSRTNTGGDTTGFGTLPPGQSLEVGRSAPYFGLPIEPSSPAAA
                     EPEAHDSQPPSSPKASTKPPRGRARSEPPPPKPTTPPTGIAAGAADIDIPSALRVGDA
                     VRVLARAVRARYSGAIAFEDDAGIRRVVLRDGDFVIAASGIDGESLVAFLSQRGDISS
                     EAASRIGRKLPQFGRHAGAALIAQGHLRQEDLWPALRSHAEWIITRIVGLARGGASLE
                     RVVPPRLAAEPSVFGGATGAEVLIEVVRRAITPSDAQSWLGGPNAELSPGESESLLAE
                     CALSEYELSLVRNASGSSIGDLIERAQVEVPELANVLFALVELGVLRARATSVSSPRK
                     ARRPPVADVIDHDAVRARIRARRALVDDGDYFALLGVSRNATSYDIRRAYSALRNEFD
                     PSNLLTPLTADLSEDLALILEILDEAYDILRDQARRDRYRRALEDTPE"
     gene            3362..3841
                     /locus_tag="VFQ35_14125"
     CDS             3362..3841
                     /locus_tag="VFQ35_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931833.1"
                     /translation="MSSFAGNSSLSLFFALAVLALAGCSTSAEESSCTPDDQDGVIGG
                     KNVVLLSVSDVDFAVGAVGSGSTQRNITVQNSSELTLTLTNVGTKPHSFVVDCISTEL
                     PAECAQTSCFPPEANIPAVMPGESTTTKFVIPVVEGAYPFKSDVAGDDVLLGQFVVN"
     gene            3838..>4623
                     /locus_tag="VFQ35_14130"
     CDS             3838..>4623
                     /locus_tag="VFQ35_14130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9931834.1"
                     /translation="MIRRRLFPSCVLVGAATLALSTSVRADVIVAEPATPIVPAFPTD
                     APSTEAQVVLQLVIDVEGRVESAVVVSRAPSNIGTTFDAAALDAVRDARFQPSTRDGR
                     PFRSRVEYVVAFHPSPPSTLRSAPDSTRAEGAAAVNATANLAPFEHPREVEVRGTGWA
                     SPRGIGDTRIERDLLDASPRQQTSEMLSAAPGFFVDHEDGEGFGNDVYLRGFDLEHGS
                     GIEMRVGSVPINSPVHVHGQGYADANFIIPEVVRSVRVLEGPYD"
BASE COUNT          757 a         1587 c         1530 g          749 t
ORIGIN      
        1 gaccgcttcg ggcgcaacgt cgagcaacgg aagcagcgca aatgctcgct ttttcagctc
       61 tgcatgtgga acgacgaggt gcggttcggc gacgctccgg ccgaagatcc aaagaatatc
      121 cagatcgagg gtccgaggtc cccaacgcac gcgtcgctcg cgtccctcgg cgcgctctat
      181 ttcgagcagg cgcgccaaga gctccgtggg agagaggcgg gcgtgtagtc gaaccgcggc
      241 gttcatgaag tcgggctgcg ggggtccgag gggaggagtc tcgtacgtgt acgagaccgc
      301 gcgcacttca gcgaactccg cgagcgcccg gactgcgcgg cggagagtct cgatgcggtc
      361 gccgagattc gagcccagcc ccacgaccac atcgatgggc tgagaaggcg acgcgtcgtt
      421 cacgaatccc ggctcggtca tgcttcaatc cgttttcact ttagcgacgc ggaagtgcct
      481 cgctgcattc gcgacgcgaa aggtcccccc gaagggttct cccgcggccg gctgcctaca
      541 aatggttccg gagtgtgatg cgcaatgagg actgatacta tgcggcggtg ttcggtttga
      601 acggaggcga actcggcctc atcattttta tcgtcgccgc gatcctgagc gcccgttatt
      661 ggccaaaaat gggcgagcgc atcgcgctgt ccctcactcg cgagcgcgac gggcgaagct
      721 gagcgtccgc gccccacgcg cctcggagtt cgcccgccgg ccggcgtcgt gatgagccgg
      781 gggtcgccag ccggccgaca cgcagcgctt gggcttcgcc cggcggatcc gccgtgcgcc
      841 ggcaggagtc tggcgggcga gggagcggct aacgtaagat gtctccgtgg cgtccgaacc
      901 cgctcccggc agcgacagtg ccggcggagc gcccagtgaa gcgctgatct tcgtgagcga
      961 cgcttcagcg gaggcagaac gactcactgc gactctccgc gcgcgaggct atctcaccgt
     1021 cgacgttccg ctcgggctgc tcgtcggtcg cgtcgccgtg cagcgccctt cactcgtgct
     1081 ctgcgacgcc gacgcgccgg gggcgctcga ggcggtggag cgaatgcggg aagctaccgg
     1141 cggccgcagc gtcgacgtgc tgttcctcgg tgaagcgggc cgcacactgg acgaccaagc
     1201 ggagcgcgtc gcccgtgagg gcagcgggat tttcgtgcgt cccgtggacg tctacgcgct
     1261 catgcgaaag gtcgaggcga tcatcggtcc ttcgagtggc cgcctcggaa ggccgtcgac
     1321 cgtaccgcac gcgaaccggg tgccggtgct ggtagcggcg actcgtcgac cttatcgcta
     1381 cgacaccaaa gcgcgtgccc gaaacttccc cgcgagcacc gccccgaccg ttgcgaccgt
     1441 gccgccacca acggcgcgtc cgaccgatgt ctcgatggcg cgtgtcccgt cggatcccgc
     1501 tgggcccgat ttcgcggcgg tagcccaggg agggctcagc gccgacttgg cggcgttgct
     1561 gggccgtgcc gaacaacgcg tgctcgggtc gcgccaattc ggaggagcag gagagcgact
     1621 ctctccagaa gcagagctcg aggcggtgct gcccgaagac ctgctggctg cgctcgacga
     1681 gccactcgat gagctcgacg acgatgacga cgacgaagac gggacccctg gcacgcacgg
     1741 ctctgattcg aacctgggaa cgcgcaccgg aagtcgaccg gggcaggact cgacggcggg
     1801 cacgtcaccc gggggctcgg taaacccatt cgctcaacgc gacgcgaaca cgacgtctgg
     1861 cggcgatcgc gaacctgccg ctccagagcc ggctccgcca acgccgccag cacacccggg
     1921 acggacggcg ccgccgagcc gcacgaacac gggcggtgac accaccgggt tcggaacact
     1981 gcccccggga caaagtctgg aagtgggtcg ttcggcgccg tacttcggcc tgccaatcga
     2041 gccctcgtcg ccggcggcgg ctgaaccaga agcgcacgac tcgcagcccc cgtcgtcgcc
     2101 caaagcttcg accaagcccc ctcgaggccg cgcgcggagt gagccccctc cccccaagcc
     2161 gaccacgcca ccgacgggca ttgcggctgg agcggctgac atcgacatcc cgagcgctct
     2221 gcgcgtgggc gacgccgtgc gcgtgttggc ccgggccgtg cgcgctcgct actcgggcgc
     2281 gatcgcgttc gaagacgacg ccggtattcg tcgagtcgtg ctgcgcgacg gcgacttcgt
     2341 gatcgccgcc tccggcatcg acggagagtc gctcgtcgct tttctttctc agcgcggcga
     2401 catctcgagc gaggccgcct cgcgcatcgg ccgcaagttg ccgcagttcg gacgtcacgc
     2461 tggcgcggcg ctcatcgcgc aaggccacct gcgccaagag gatctgtggc ccgcgttgcg
     2521 ctcgcacgcg gagtggatca tcacgcgcat cgtgggcctc gcgcggggcg gagcgagcct
     2581 cgagcgcgtg gttccaccga gactcgcggc cgagccatcg gtcttcggcg gcgccaccgg
     2641 cgccgaggtg ctcatcgaag tcgtgcgccg cgccatcacc ccgagcgacg ctcagagctg
     2701 gctcggcggg cccaacgcgg agctcagccc cggtgaatcg gagtcgctgc tcgcggagtg
     2761 cgccctttcc gaatacgagc tcagcctcgt tcgcaacgcg agcggcagca gcatcggcga
     2821 tctcatcgag cgcgcccaag tcgaagtgcc ggagctcgcg aacgtgctct tcgcgctggt
     2881 ggagctcggc gtgttgcgcg cgcgggccac gagcgtgagc tcgccgcgaa aagcgcgccg
     2941 gcctcccgtc gccgacgtca tcgaccacga cgcggtgcgc gcccgcatcc gtgcccggcg
     3001 agccctggtc gacgacggtg actacttcgc gttgctcggc gtttcgcgaa acgcgaccag
     3061 ctacgacatc cgtcgagcgt attcagcgct tcgcaacgag ttcgacccgt cgaacctgct
     3121 cacgccgctg acggctgacc tcagcgaaga cttggcgctc attttggaga ttctggacga
     3181 ggcttacgac atcttgcggg accaggcccg acgcgaccgc taccgacgtg cgctcgagga
     3241 caccccggag taagcacgag ccggccgcgg gctcgatgga cgcgagcgca ttcgcctgcc
     3301 gcctcgccat ccgataaatg tcggataccg accacgattg gcgcgcgtta ccttccgccc
     3361 gatgagctcc tttgccggaa acagcagcct ttctctgttt ttcgcgctcg cagtgctcgc
     3421 tctcgccggg tgcagcacgt ccgccgagga gagttcctgc accccggacg accaagacgg
     3481 cgtgatcgga ggcaagaacg tcgtactgct cagcgtcagc gatgtggatt tcgcggtcgg
     3541 agcggtcggc agcggctcga cacaacgcaa tatcaccgtt caaaactcga gcgagctcac
     3601 gctcacgctc acgaacgtcg ggaccaagcc gcactcgttc gtggtcgact gcatctcgac
     3661 cgagctcccg gctgagtgcg ctcagacttc gtgctttcca ccggaagcca acatccccgc
     3721 cgtgatgcct ggcgaaagca cgacgaccaa gttcgtaatc ccggtggtag agggcgccta
     3781 tccgttcaag tccgacgtcg cgggcgacga cgtcttgctc ggccaattcg tggtcaattg
     3841 attcgtcgtc ggctcttccc ttcgtgtgtt ctggtcggcg ccgcgacgct cgcgctttcg
     3901 acgagcgttc gcgctgacgt catcgtggcc gaacccgcga cgcccatcgt tccggcattt
     3961 ccgaccgacg cgccatcgac cgaagcgcag gtcgtccttc agctcgtcat cgacgtcgaa
     4021 ggacgggtgg agtcggcggt cgtggtgagc cgcgctccat cgaacatcgg aacgacattc
     4081 gatgcggccg cgctcgatgc ggtacgcgat gcccggtttc agccctccac gagggacggg
     4141 cgtccgtttc ggtcacgagt cgagtacgtc gtcgcttttc atccgtcgcc gccctctacc
     4201 cttcggagcg cgcccgactc gacccgcgcc gagggggccg ccgccgtgaa cgccacggcg
     4261 aacctggctc cgttcgagca cccgcgcgaa gtcgaggtgc gcggcaccgg ttgggcttcg
     4321 ccgcggggaa tcggcgacac tcgcatcgag cgcgacttgc tcgacgcctc tccgcgccaa
     4381 caaacgagtg aaatgctgtc cgcggcaccg gggttcttcg tcgatcacga agatggtgag
     4441 ggcttcggaa acgacgtcta cctgcgcggg ttcgatctcg agcacggctc cgggatcgaa
     4501 atgcgcgtcg gcagcgtacc gatcaacagc cccgtccacg tgcacggcca aggctacgcc
     4561 gacgctaact tcatcatccc cgaggtcgtg cgcagcgtac gggttttaga agggccgtac
     4621 gac
//
LOCUS       DASTKS010000686         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1671842, whole genome shotgun sequence.
ACCESSION   DASTKS010000686 DASTKS010000000
VERSION     DASTKS010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671842"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFQ35_14135"
     CDS             complement(<1..422)
                     /locus_tag="VFQ35_14135"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931835.1"
                     /translation="MSAATESPEIPELLAERETWSEHMENDPAESPAPNTLSLPFLEA
                     LYADYLKDPASVPPTYRDYFAGVEHDERFSRSPRIGPAFRPSSVFNPETRVNGHAAHA
                     TVGRAPGASNGVSDAAVRQDRLDALVRAYRVRGHMIAK"
     gene            1114..2544
                     /locus_tag="VFQ35_14140"
     CDS             1114..2544
                     /locus_tag="VFQ35_14140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931836.1"
                     /translation="MSVKSFQPGTILADKYRVERVLGEGAMGVVLLARHLQLDEFVAI
                     KLPLSTVLESADAISRFLREARAAVKIKSEYVARVSDVGTLETGEPYIVMEYLQGQDL
                     ATLLRQRGQLPVEQALEFVLQACEALAEAHALGIVHRDLKPANLFCIRRADGVLAVKV
                     LDFGISKASSAGFDATLTRTGTLMGSPLYMSPEQLISARDVDARSDIWSLGIILFQLL
                     SGRAPFNGETLPALSMAIAHAPPESLRSLRPELPTGLERVVLKCLEKDRERRYRDVGE
                     LAKALLEFAPKRATASVERISRIVSLAASPAAPDTARPADAAFAATQTQDTLSNFGQS
                     RVASAKRAPTALLAGVVSLLGIAGAAWYWMRSTVPLPPTPMLVNSVAATASSGLVKNV
                     GAAIPQASTPAPPVASTAPPHGEAPPVAAPAPSTAPRANPAPPALPHVKRPPQTSTSN
                     CNPNFFLDDQGEKHFKPECFVAPSGH"
     gene            2552..>3080
                     /locus_tag="VFQ35_14145"
     CDS             2552..>3080
                     /locus_tag="VFQ35_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931837.1"
                     /translation="MRNFARTSSLAVLVAGATAAAHRAHAAEPTVSECLGANEKSIAL
                     RRESKLEAAREQLLVCAAASCPAEIRKECADRVERVNAAIPTIVFEVKDAVGIDVVAV
                     HLTVDGRPATDVKEGVALPLDPGTHTFQFEANGYLRSEKTLVLRQGEKDRRELITLAR
                     EPVKEARAATVPAALA"
BASE COUNT          580 a         1040 c          965 g          495 t
ORIGIN      
        1 atcttcgcga tcatgtggcc gcgcacgcgg taagcgcgaa cgagcgcgtc cagccgatcc
       61 tgccgcaccg ccgcgtccga aactccgttg ctggcgccgg gcgcgcgccc caccgtcgcg
      121 tgcgcggcat gcccgttcac ccgcgtttcg gggttgaaca cgctcgaggg gcggaaagca
      181 gggccaatcc gcggagaacg cgagaaccgc tcgtcgtgtt cgacacccgc gaagtagtcg
      241 cggtaggtgg gcggaacgct tgccggatcc ttcaggtagt cggcgtacag cgcttccaag
      301 aacgggaggc tcaacgtgtt cggtgccgga ctctcggcgg ggtcattttc catgtgttcg
      361 gaccaggttt ctcgctcggc caaaagctcc gggatttcgg gcgactcagt agcggcactc
      421 acgaggaaga cctttagttt gcgggagccc caggtggctc ggcgcgatga aagcgagcat
      481 tgaatgggcc gtgtcttcga gtgggtcaag gcacggcacg agcagcgatg cacaattagc
      541 taccgccccg aaacggccga cccgtcagca cgcaacctag cagaaacatt caaggcccag
      601 cgtgtgccac ttggccgctc tgcttccatt tttcggcgcg gctcggaaag gacaccgctc
      661 atataaggct gctgagccgt gagcgccagc cccgcggccg aaggggcgtc gaaagccgac
      721 cgcgttcgcc gcgaagctgg gcgcggccat cctttgagga cacccgcgcg atccgcagac
      781 tccacatgga acctggcgcg ctccgccagc gcttgccggg cgttgagtct ccgccatcaa
      841 gcgccgactc aacgccactc gaaagccgtt cgcgacgttc ccaacgcttt cccacagcgg
      901 cccaacgccg cgagccaagc tccacgaaag cgaccgcggc cgaagctcgt cgcgactcga
      961 tcgctaaggt ctcacctcgg caaaatgact atgcctcacc cgaagtcgga ctcgaccgaa
     1021 cgtcgtctcg acaactcgaa atgatcatct ccgttgcgtt ctgacgcgaa gctctggaag
     1081 atgaatccgt tctcaacggg gattcgatcg gtcgtgagcg tcaaaagctt tcaacccggc
     1141 acgatcttgg ctgacaagta tcgcgtggag cgcgttcttg gcgagggcgc aatgggcgtc
     1201 gtgctactcg cccgccacct tcagctcgat gagttcgtcg ccatcaagct gccgctgtcg
     1261 acggtactgg agtctgccga cgccatcagt cgattcctgc gagaagcgcg cgcggcggtg
     1321 aagatcaaga gcgagtacgt cgcgcgggtg agcgacgtgg gcactctcga aacgggcgag
     1381 ccctacatcg taatggagta tctccaggga caagatctgg cgacgctgct caggcagcga
     1441 ggtcagttgc cggtagaaca agcgctcgag ttcgtcctgc aagcgtgcga ggcgctggcc
     1501 gaagcccacg cgctcggcat cgtgcacagg gacctgaagc cggcaaatct gttctgcatt
     1561 cgacgcgcgg acggagtctt ggccgtgaag gtgctcgact tcggaatttc caaggcgagc
     1621 tctgcggggt tcgatgcgac actgacgaga accggaacgc tgatgggctc gccgctctac
     1681 atgtccccgg agcagctcat ttctgcgcgc gacgtcgacg cgcgcagcga tatctggtcg
     1741 ctcggaatca ttctgttcca gctcttgagc ggacgagcgc ccttcaacgg cgagacctta
     1801 cccgcgctga gcatggccat cgctcacgcc ccgcccgagt cgctacgcag cctccgaccc
     1861 gagttgccta cagggctcga acgagtcgtc ctcaagtgcc tcgagaaaga tcgagagcgg
     1921 cgctaccgcg acgttggcga gctcgcgaag gcactgctcg aattcgcgcc caagcgagca
     1981 acggcgtcgg tcgagagaat atcgcgcatc gtctcgctcg ccgcatctcc agccgcgccg
     2041 gacacggcac gacctgcgga cgcggcgttc gcggcgacac aaacccagga cacgttgtcg
     2101 aacttcgggc aaagccgcgt ggcgtccgcg aaacgcgcac cgacggcgct cctagccggc
     2161 gttgtgagct tgctcggtat cgcgggggcc gcatggtact ggatgcgttc gacggtaccg
     2221 ttgccgccga cgccgatgct ggtcaactcc gtcgcggcaa cggcgagctc cgggctcgtc
     2281 aagaacgtgg gcgcggcgat ccctcaggcg agtactcccg cgccccccgt agcttcgacc
     2341 gcgccgccgc acggtgaggc tccgcccgtg gccgcgcccg cgccgtcgac cgcgccacgc
     2401 gcgaatccgg ccccgccggc gctgccgcac gtgaagcggc ctccgcaaac gtcgacctcg
     2461 aactgtaacc ccaacttctt cctcgacgac caaggggaaa agcacttcaa gcccgagtgc
     2521 ttcgtcgcac ccagcggaca ctgagcaagc catgcgaaac ttcgcgcgca cctcgtctct
     2581 cgcagtgctg gtggccgggg ccacagctgc tgctcatcga gcccacgcgg cggagccgac
     2641 cgtctcggag tgcctcggag cgaacgagaa gtcgatcgcg ctgcgtcggg agtccaagct
     2701 cgaagccgcg agagagcagc tgctcgtgtg cgcggccgcg agctgccccg ccgaaatccg
     2761 caaggagtgc gccgaccgcg tcgaaagagt caacgccgcg atcccgacca tcgtcttcga
     2821 agtcaaagac gcggtcggca tcgacgtggt cgccgttcac ctcaccgtcg acggccgacc
     2881 cgccaccgac gtgaaggaag gcgtggctct cccgctcgac ccgggcacgc acaccttcca
     2941 attcgaggca aacggctacc tccgatccga gaagactctc gtgctgcgac aaggcgaaaa
     3001 agaccgacgc gagctcatca cgctggcgcg cgaaccagta aaggaagctc gagcagccac
     3061 cgtgcctgct gccttggccc
//
LOCUS       DASTKS010000687         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_778419, whole genome shotgun sequence.
ACCESSION   DASTKS010000687 DASTKS010000000
VERSION     DASTKS010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778419"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VFQ35_14150"
     CDS             complement(<1..1171)
                     /locus_tag="VFQ35_14150"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9931838.1"
                     /translation="MIRSPLEPEPRLHPSEPPPAVSAAPAPRVSKPSVPLAAPRELPP
                     VTVGIRVKLLALMVATISAVVIALATYFPARQITEVRAGLKERASVYAGLASQQLRSA
                     VAFNDQETAREVLAAVAKDPLIDSIAVYTAQGKRLHGEGHVSELAFAARSLDERRVFA
                     LPGRVLAVAPVVSLEGPKGTVVIELSTRAASQARNRLVWVALAAGASALLAAAGLVWL
                     IARSLAERVERIALVATAVAKGDLSQTLDTEGPSDEIGVLSHGFNAMLDQLRHLIGHI
                     RATAREEKARLERLVAERTVALDRKNQDLKLVLDNVDQGFVTIDRDARLVGEHSRIIE
                     SWLGDVQNEQNLWDYLERAAPGARLNFSLCWTEVTDAVLPLEVTLHQMPRKLEADG"
     gene            1294..>2257
                     /locus_tag="VFQ35_14155"
     CDS             1294..>2257
                     /locus_tag="VFQ35_14155"
                     /inference="COORDINATES: protein motif:HMM:NF041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6577-like cysteine-rich protein"
                     /protein_id="HET9931839.1"
                     /translation="MRSVFIAVRLLGLERAVRGLSAWALVCFSCVSVLGCQSNDALFD
                     GSTGNGSTCDAPLLRCANRCVDLFSDVSNCGGCGIACEDGLSCVDGECGLGCVGGTTS
                     CGERCVDTNVDPKHCGACDKACSDGEVCLDGSCELRCASGNQACAGRCVDLASDPQNC
                     GTCGKACQTGEVCSSGHCAASCLGGSSQCGARCVDLGSDPNNCGSCGNKCDLEKEVCV
                     EGQCGVSCLGGTTQCGNRCVDLNWDPENCSACDNACNPGEVCTQGSCGVQCLGGPSQC
                     GGRCVDLEGDRKNCGSCDNACGTGEVCLLGHCIACLGGTKQCGSR"
BASE COUNT          358 a          709 c          797 g          393 t
ORIGIN      
        1 gcccgtccgc ctcgagcttg cgcggcattt ggtgcaacgt gacctccagc ggcaacaccg
       61 cgtccgtcac ctcggtccag cagagcgaga agttgagacg cgcgcccggg gccgctcgct
      121 cgaggtagtc ccacaggttc tgctcgttct gaacgtcgcc gagccagctc tcgatgatgc
      181 gcgagtgctc gccgacgagg cgcgcgtcgc ggtcgatggt gacgaaccct tgatcgacgt
      241 tgtcgagcac gagcttcaag tcttggttct tgcgatcgag cgcgaccgtg cgctcggcca
      301 cgagccgctc gaggcgcgcc ttttcctcgc gcgcggtcgc gcgaatgtga ccgatgaggt
      361 ggcgcagctg gtcgagcatg gcgttgaagc catgggagag aacgccgatt tcgtcgctgg
      421 ggccttccgt gtcgagggtt tggctcaagt cgccctttgc cactgccgtc gcgacgaggg
      481 cgattcgttc cacgcgctct gcgagtgagc gcgcgatgag ccagaccagc ccggcggcgg
      541 cgagcagcgc cgatgcgccc gccgcgagcg cgacccacac caggcgattg cgcgcttggc
      601 tcgccgcgcg cgtcgagagc tcgatcacga ccgtgccctt gggcccctcg aggctcacca
      661 ccggcgcgac cgcgaggact cggcctggca gagcgaacac gcgccgttcg tccaggctgc
      721 gcgctgcgaa ggcgagctcg ctcacgtggc cttccccgtg caagcgcttg ccttgcgcgg
      781 tgtacacggc gatgctgtcg atgagcggat ccttggcgac cgccgcgagc acctcacgcg
      841 ccgtctcttg gtcgttgaag gcgaccgccg agcgaagctg ctggctggcg agccctgcgt
      901 acaccgaagc cctctccttc aaccccgcgc ggacctcggt gatctgacgt gcggggaagt
      961 aggtggcgag cgcaatgacc accgctgaaa tcgtcgccac catcagcgcc aggagcttta
     1021 cccggatccc gacggtcacc ggcggaagct cgcgcggcgc cgcgagcggc accgacggct
     1081 tcgataccct cggcgcggga gccgcactga cggcgggtgg cggttcggag ggatggagcc
     1141 gtggctccgg ttcgagaggt gatcgaatca tcgagaaggc cagaacggcg accctgaggc
     1201 gtgcttgagt gcgtctgcgc gaagtcgcgt acgagttttc ggaatcgacg ggagggcctc
     1261 aggtggcgcc cttcgacggc cgtagtgcga ggtgtgagga gtgtcttcat cgcggtccga
     1321 ttgctcggcc tcgaacgcgc ggtgcgcggg ctttcggcct gggcgctggt ttgtttttca
     1381 tgcgtcagcg tcctcggctg tcaaagcaac gacgcgctgt tcgatggctc gacgggaaac
     1441 ggctccacgt gtgacgcacc gcttctccgc tgcgcgaatc gctgcgtcga cttgttcagc
     1501 gacgtttcga actgcggcgg atgcgggatc gcctgtgaag atggcctgag ctgcgtagac
     1561 ggcgagtgcg ggctcgggtg tgtcggtggg acgacctcgt gcggagagcg ctgcgtcgac
     1621 accaacgtcg atccgaagca ctgcggagcg tgcgacaagg cgtgctccga cggcgaagtg
     1681 tgcctcgacg gaagctgcga gctccgctgt gcgagcggca accaagcctg cgctggtcgc
     1741 tgcgtcgatc tggcgagcga ccctcagaac tgtggaactt gcggcaaggc ttgccaaacc
     1801 ggcgaggtct gcagcagcgg ccactgtgcg gcgagctgtc tcggcggtag cagccagtgc
     1861 ggtgcgcgtt gcgtcgactt gggttcggat ccgaacaact gcggaagctg cggcaacaag
     1921 tgcgatctcg aaaaagaagt ttgcgtcgaa ggccaatgcg gcgtcagctg cctcggcggc
     1981 accacgcaat gcgggaaccg ctgcgtggat ctgaattggg accccgagaa ctgcagtgct
     2041 tgcgataacg cctgcaaccc cggcgaagtg tgcactcagg gaagctgcgg ggtccagtgc
     2101 ttgggcggcc cttcgcaatg cggcggccgc tgcgtcgatc tcgaaggcga ccgcaagaat
     2161 tgcggtagtt gtgacaatgc ctgcggtacc ggggaggtgt gcctgctcgg acattgcatc
     2221 gcctgcctcg gcggcaccaa gcagtgtggc tcgcgct
//
LOCUS       DASTKS010000688         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_332733, whole genome shotgun sequence.
ACCESSION   DASTKS010000688 DASTKS010000000
VERSION     DASTKS010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332733"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..686
                     /locus_tag="VFQ35_14160"
     CDS             141..686
                     /locus_tag="VFQ35_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931840.1"
                     /translation="MKSFWVLGIVAATSVIGCGAVEEGQEPLGPLRNGSGGAHSSAGT
                     GGNRYASSSGGDRSNASGGAPVLPASGGASPIATSGGGGSAPSAPASGGGGAGGGFAL
                     GTSGAGVGGRGLFFGTGGASFATGGVRNTAGSPQGGFTTTDPDFETHIDLNGDGWIDL
                     NPPSGESGEGGAGISIDWGAP"
     gene            828..>2348
                     /locus_tag="VFQ35_14165"
     CDS             828..>2348
                     /locus_tag="VFQ35_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HET9931841.1"
                     /translation="MVAAPLYDCLPPEAETTPDSILEGFLRYVSELGLELYPHQEESI
                     LAVVADNNVILNTPTGSGKSLVASAVHFNALAKGHRSFYTCPIKALVSEKFFALCREL
                     GPDRVGMMTGDATVNRDASVICCTAEVLANIALAEGDKANVDWVVMDEFHYFSDRDRG
                     VAWQIPLLTLKSARFVLMSATLGDVSEFERVLRQHTGADVVTVKSVERPVPLEFEYQE
                     KQLHEAVYSLAQSGRAPVYVVNFTQRACAEAAQSLLSLDFATKEEKKKLAELLAKESF
                     RSPNGKDMQKLLRHGIGLHHAGLLPRYRLLVEKLAQRGLLKIICGTDTLGVGVNVPIR
                     TVLFTKLCKYDGDKVKILSVRDFKQIAGRAGRRGFDERGYVVALAPEHVVENMRLEAK
                     AASDKFNKKKKFVRKKPPDWGYVHWDKSTFDKLVESEPEALVSRFSVSPGMLLQVLGR
                     PEGGCRAMKNLVRDSLEAPGAKRRHGKHALVLLRSLWQAGVVDFRPLSEGGGVRVNL"
BASE COUNT          436 a          686 c          826 g          400 t
ORIGIN      
        1 gagcgcaacc atatggccgc ggagtggaac ctgcagctag cgctcgggcg agaccccgag
       61 aaggtgctgg ggagctcgcc accttggctt cagtgaacgt gagcgcgcgg atcgcgcaat
      121 cacttagagg agtgagaaaa atgaagagtt tttgggtgct gggaatcgtt gcggcaacct
      181 ccgtcatcgg gtgcggggcc gtggaagaag gacaagaacc gttgggtccg ctccggaatg
      241 gatcgggagg cgcgcactcg agcgcgggaa ccggcggcaa tcgctacgcc tcgtcgagcg
      301 gcggcgatcg ttcgaatgca tctggcggag ctcccgtcct ccctgcgagc gggggagcgt
      361 cgccgatcgc gacgagcgga ggaggcggca gcgcgccctc tgctcccgcg agcgggggag
      421 gcggagcggg cggcggattt gcgctcggta ccagcggcgc gggcgtgggc ggcaggggtc
      481 tcttcttcgg cactggtggc gcgtcgttcg cgactggcgg cgtacgaaat accgcgggtt
      541 cgccccaagg aggtttcacg accaccgacc ctgatttcga aacgcacatc gacttgaacg
      601 gagatggctg gattgacttg aaccctccat ccggtgaaag cggagagggc ggggcgggaa
      661 tatccatcga ctggggcgcg ccctgaagag cgctcaccgt gcctgccttt attgcgccgg
      721 cgttggagtg cgccggcgcg tgagcgagaa gttctggacc gggggcgagc tgcgttccga
      781 ggaagaggtt gcggcgctgg cggtgacgtg agatgcgcgg caattccatg gtcgccgcgc
      841 ccctgtacga ttgtttgccc cccgaggccg aaaccacgcc ggatagcatc ctggaagggt
      901 tccttcggta cgtcagcgag ctcgggctgg agctctaccc tcaccaagaa gagtcgattt
      961 tggcggtggt ggcggacaac aacgtcatcc tgaacacgcc gaccggctcc gggaaatcgc
     1021 tggtcgcgtc ggcggtgcat ttcaacgctc tcgccaaggg acatcgctcg ttttacacgt
     1081 gcccgatcaa ggcgctcgtg agcgagaagt ttttcgcgct ctgtcgtgag ctcggcccgg
     1141 atcgagtcgg catgatgacc ggcgacgcga ccgtgaaccg cgacgcgagc gtgatttgct
     1201 gcacggcgga ggtgctggcg aacatcgcgc tcgccgaggg agacaaggcc aacgtcgact
     1261 gggtagtcat ggacgagttc cactacttct cggatcgtga tcggggagtc gcgtggcaaa
     1321 tcccgctgct cacgttgaag agcgcgcgct tcgtgctcat gagcgcgacg ctcggcgacg
     1381 tgagcgagtt cgagcgggtc ctgcggcagc acaccggcgc ggacgtcgtc accgtgaaat
     1441 cggtggagcg gcccgtgccg ctcgagttcg aatatcaaga gaagcagctg cacgaagcgg
     1501 tgtactcgct cgctcaaagc ggccgcgcac ccgtctacgt cgtgaacttc acgcagcgcg
     1561 cctgcgccga agccgcgcaa agcctgctca gtttggattt tgcgaccaag gaagagaaga
     1621 agaagctcgc ggagctcttg gccaaagaga gcttccgcag cccaaacggc aaggacatgc
     1681 agaagctcct tcgccacggg attggcttgc accacgccgg gctcctccct cgctatcggc
     1741 tcctcgtcga aaagttggct cagcgcgggc tcctgaagat catctgcggg accgacacgc
     1801 tcggcgtggg cgtgaacgta ccgattcgga cggtgttgtt cacgaagctc tgcaagtacg
     1861 acggtgacaa ggtcaagatc ctcagcgtgc gcgacttcaa gcaaatcgcc gggcgcgcgg
     1921 ggcgtcgcgg cttcgacgaa cgcggctacg tcgtggccct cgcgccggag cacgtcgtcg
     1981 aaaacatgcg gctcgaagcc aaggcggcct cggacaagtt caacaagaag aagaagttcg
     2041 ttcgcaagaa gccgcccgac tggggctacg tgcactggga caagagcacc ttcgacaagc
     2101 tcgtcgagtc ggagcccgag gccttggtct cgcgcttcag cgtgagcccg gggatgttgc
     2161 tgcaagtgtt ggggcgacca gaaggtggct gccgcgcgat gaaaaacttg gtgcgtgaca
     2221 gcctggaagc gcctggcgcg aagcggcgcc acggcaagca tgcgctcgtg ctcttgcgct
     2281 cgctgtggca ggcgggcgtc gtcgattttc gaccattgag cgaggggggc ggtgtgcggg
     2341 tcaacctc
//
LOCUS       DASTKS010000689         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1896347, whole genome shotgun sequence.
ACCESSION   DASTKS010000689 DASTKS010000000
VERSION     DASTKS010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896347"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFQ35_14170"
     CDS             <1..222
                     /locus_tag="VFQ35_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931842.1"
                     /translation="QEDRLNSAKSKGLVSTIGFGVGAAGIVLGTVFLVTSSGSSSSSA
                     SNSSAPPTAWRVRPKAAIGLGSVQLGADF"
     gene            293..760
                     /gene="greB"
                     /locus_tag="VFQ35_14175"
     CDS             293..760
                     /gene="greB"
                     /locus_tag="VFQ35_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET9931843.1"
                     /translation="MPNYLTPEGAKKYAEELNKLLSVDRPRVVQEVADAAAQGDRSEN
                     AEYTYGKKRLREIDRRVRFLTKRLDAAVVVRSEEVRKIDEVRFGATVEVVDENGKKSR
                     YQVVGPDEADPSTGRISFKSPLGASLMKKKVGDVIAVQRPAGEIEIEILEIEY"
     gene            complement(789..1613)
                     /locus_tag="VFQ35_14180"
     CDS             complement(789..1613)
                     /locus_tag="VFQ35_14180"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9931844.1"
                     /translation="MASLRDKTLFITGATRGIGRAIALRAARDGANVAVLGKTDEPHP
                     KLSGTVHDVVREIEAAGGRAIPCVTDIRFEDQVERAVADTLAAFGGIDVVVNNASAIS
                     LTTTAATSMKRFDLMQQVNVRGTFLCSKACLPALEKASNPHILMLSPPLSLKPAYYGA
                     HLAYSLSKFGMSFCVLGLAEELRPLGIAVNALWPRTVIATAAVENLLGGAATMARARK
                     PEIVADAAHAIVTRPSREFSGNFCIDEQVLREAGVEDFSQYAFAEGTELLTDLFID"
     gene            complement(1642..2697)
                     /locus_tag="VFQ35_14185"
     CDS             complement(1642..2697)
                     /locus_tag="VFQ35_14185"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9931845.1"
                     /translation="MVARPQNFGNVTGATKMAQSVAARVFRGDGVEAWHAASVAVVTP
                     EGLTKTFGDPQLASLARSTIKPFQALALLTSGAAEAFGFDDREIALACASHNGDDEHR
                     QVAERMLAKARVPASALGCGAHVPLGMRLRETPVNDGEDADPLRNNCSGKHAGFLALC
                     RHLEEPLENYLEPGSATQATVLRELARACDVDAESLPRGIDGCSAPNYALPLSALAAG
                     MLRLARPETAPEPLRAALERVRRSMLQHPELVSGPGRFDYALSCALPERLLVKSGAEA
                     MQIGACLDPPLAFAIKVHDGGARALPPICIAVLRQLGLLGSELAPGLVKYEAPVTTNH
                     KKVITGRIEATLELEPR"
     gene            2757..2903
                     /locus_tag="VFQ35_14190"
     CDS             2757..2903
                     /locus_tag="VFQ35_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931846.1"
                     /translation="MRKPELDGSERDRPGISVSKPGALLIALVLSAAGTGYAIYWLIR
                     SARG"
     gene            2925..3404
                     /locus_tag="VFQ35_14195"
     CDS             2925..3404
                     /locus_tag="VFQ35_14195"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET9931847.1"
                     /translation="MVRVCFVCLGNICRSPSAEGVMRYLVSEAGLEHAIEVDSAGTAG
                     YHSGEVPDARARAAGRRRGIEIKGRARRIEPLDFERFDYVLAMDAANLEDLLSIAPES
                     AKAKVRLLRSFDPASAPGSSVPDPYYGGDDGFDEVLDQCLAACAGLLNHLRREHSLR"
     gene            3401..4264
                     /locus_tag="VFQ35_14200"
     CDS             3401..4264
                     /locus_tag="VFQ35_14200"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET9931848.1"
                     /translation="MRPELESSLQRRLGVHHLRATAISGGDINDAFRIDCAKGTLFVK
                     ANARAPRGLFEAEARGLAWLSEASALRIPNVVAVSDPDEPVSYLVLEYIEPGRPARDF
                     DQALARGLAKLHRAGGERLGLDHDNFIGSLPQSNAAAPSWAEFYVSRRLAPLLERAAA
                     TGKASYAMRRGFERLFSVLPGLVGPEEPPSRLHGDLWSGNLHVAEDGAPCLIDPAVYA
                     GHREMDLAMMRLFGGFSERVFSAYHEAHPLASGVEERVALYQLYPLMVHVNLFGGSYV
                     TSVERALSRYV"
     gene            4486..>5932
                     /locus_tag="VFQ35_14205"
     CDS             4486..>5932
                     /locus_tag="VFQ35_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HET9931849.1"
                     /translation="MRRAACFVMLGFVASACGEGGSKPSSSTQSDISAPTNRYPDAPP
                     NAPAPPAACGDMNGGGDQHVDFAALMAQKVSEKPEVEARQSTLLMDRYDLSDNPHPTV
                     RMSAGKAVQRGARVKLPSGASFDALAFMTPAEIRSQDVFPLGFYPLPHPKQPEGGMVF
                     PHFHIEEVLRQTGRDLTRFDLDFDIPDTFLPEFPPPIYLTTHPELGDVSQGQLVTTDN
                     YYDLFKCILNPKQLDGLRLLLTPFPQQQFNPIDDRRSARPSLGVACFDCHANGHTNAA
                     THLVGDIRPQELRHRIDSVSLRGVNIQRLFGSQRALMSIEDFTEFEQRAAYFDGDPVL
                     ATKKGMNILDRGAQVQTMAEFQKILDFPPAPKLGLDGMLNQEVATAAEIRGQTLFFGK
                     ARCSTCHAPPYYTDLTMHDLELERFFAPKMINGIMANGDGPIKTFALRGLKDSPPYLH
                     DGRLLTIEDTVEFFNLVLGTRLTTQEKSDLTE"
BASE COUNT         1044 a         1888 c         1955 g         1045 t
ORIGIN      
        1 caagaagatc gattgaactc ggcgaagtcc aaaggcctgg tttcgaccat cggcttcgga
       61 gtcggtgcgg cgggaatcgt actcggtacc gtctttctcg tgacgagcag cggttcgtcg
      121 tcgtcgagcg caagcaacag cagcgcgcct cccaccgcgt ggcgagtgcg tccgaaggcg
      181 gcaatcggcc tcggaagcgt gcagctcggg gccgacttct gagctgattt cgcggcgaac
      241 gggtctcgtc acaggcgcgc tgatttgcgc tacggtggcg ggattcgttg ccatgccgaa
      301 ctacctaacc cccgagggtg cgaagaagta cgccgaagag ttgaacaagc tgctcagtgt
      361 ggaccggccc cgcgtggtgc aagaggtcgc cgacgcagcc gcgcagggtg atcgctccga
      421 aaacgcggag tacacgtacg ggaagaagcg cctgcgtgaa atcgatcggc gcgtccgctt
      481 tctgaccaag cggctcgacg ccgccgtcgt cgtgcgcagc gaggaagttc ggaagatcga
      541 cgaggtgcgt ttcggcgcga ccgtcgaggt cgtcgacgaa aacggcaaga agagccggta
      601 ccaagtcgtg gggcccgacg aggcggaccc gagcactggg cgcatcagct tcaagtcgcc
      661 gctcggcgcg agcctcatga agaagaaggt cggcgacgtg atcgcggtgc agcgtcccgc
      721 cggcgaaatc gaaatcgaga tcctcgaaat cgagtactga gcgtagcgcc tgcgccgagt
      781 cggcccgctc agtcgatgaa taggtcggtg aggagctcgg tgccttcggc gaaagcgtat
      841 tgcgagaagt cctctacgcc ggcttcacgc aagacctgct cgtcgatgca aaagttaccg
      901 gagaactcgc ggcttgggcg agtcacgatg gcgtgcgctg cgtccgcgac gatctccggt
      961 ttgcgtgcgc gcgccatcgt ggcggcaccc ccgagcaggt tttcgacggc agcggtggcg
     1021 atgacggtgc gcggccagag cgcgttcacc gcgatcccga gcgggcgtag ctcttcggcg
     1081 agcccgagca cgcaaaagct cattccgaac ttcgagaggc tgtaggcgag gtgcgcgccg
     1141 tagtacgcgg gcttcagcga aagcggcggc gagagcatca aaatgtgggg atttgaagcc
     1201 ttctcgagcg cgggcaggca cgctttggag cacaaaaagg tgccgcgcac gttcacttgc
     1261 tgcatgaggt cgaaacgctt catgctcgtg gccgcggtcg tggtcaggct gatcgcgctc
     1321 gcgttgttca ccacgacgtc gatgccgccg aacgcagcga gcgtgtcggc gacggcgcgt
     1381 tcgacctgat cttcgaagcg gatgtcggtc acgcagggga tggcgcggcc acccgccgcc
     1441 tcgatctcac gcaccacgtc gtggacggtt ccagagagct ttgggtgggg ctcgtcggtc
     1501 ttgccgagca ccgcgacgtt ggcgccgtcg cgcgccgcgc gcagcgcgat ggctcgcccg
     1561 atgcctcggg tcgcgccggt gatgaacaag gtcttgtctc gaagtgacgc catggctcct
     1621 cgttcgtgcg ccgccggtgg ttcagcgggg ttcgagctcc agcgtagcct cgattcgccc
     1681 cgtgatcacc ttcttgtgat tcgtagtgac cggcgcctcg tacttcacga gcccaggcgc
     1741 gagctcactg cccagtagcc cgagctgccg caggaccgcg atgcagatgg gcggcagcgc
     1801 tcgggcccct ccgtcgtgga ctttgatcgc gaaggcaagc ggcggatcca ggcaggcgcc
     1861 gatctgcatc gcctcggcgc cgctcttcac gagcagacgc tcggggagtg cgcacgagag
     1921 cgcgtagtcg aagcgccccg ggccggacac gagctcggga tgttgcagca tcgagcgacg
     1981 gacgcgctcc agggctgcac gcagcggctc gggggcggtt tcgggccgag cgagccggag
     2041 cattcccgcc gcgagcgccg agagtggcaa tgcgtagttc ggcgcgctgc agccgtcgat
     2101 gccacgcggc aggctctcgg catccacgtc acaagcgcgc gcgagctcgc gcagcacggt
     2161 cgcctgcgta gccgagcccg gttccagata attctcgagc ggctcttcca gatgacgaca
     2221 cagcgcgagg aacccggcgt gcttgcccga acagttgttg cgcagcggat cggcgtcttc
     2281 cccgtcgttg accggtgttt ctctgagtct catcccgagc ggtacgtgcg cgccgcagcc
     2341 gagcgcggag gcggggaccc gcgccttggc gagcatgcgt tcggcgacct gccggtgctc
     2401 gtcgtcgccg ttgtgcgacg cgcacgcgag cgcgatttcg cgatcgtcga agccgaatgc
     2461 ttccgcggca ccgctcgtca agagagcaag cgcttgaaac ggtttgatgg tcgatcgcgc
     2521 aaggctcgcg agctgcgggt cgccgaaagt tttcgtcagc ccttccggag tgacgacggc
     2581 tacggacgcg gcgtgccatg cctcgacgcc atcaccccga aacacgcgcg cggcgacgct
     2641 ctgcgccatc ttggttgctc cggtcacgtt gccaaaattc tgcggtcgcg cgaccacgga
     2701 agcaacgctt tacgagggtc gagaactcgt tcaaggtgcg agcgcgagga gaatcgatga
     2761 gaaagcccga gctcgatggt tccgagcgtg accgtcccgg catttcggtc agtaagccag
     2821 gagcgctctt gatcgcgctc gtactcagtg ctgccggcac gggttatgcg atctactggc
     2881 tgattcgctc ggctcgtgga tgagaagtga gctaggctga cagcgtggtt cgcgtgtgtt
     2941 tcgtgtgttt gggaaacatc tgtcgctcgc cgagcgccga aggcgtgatg cgttacttgg
     3001 tgtccgaggc cggtctcgag cacgccatcg aggtggatag cgccggtact gccggatatc
     3061 actcgggtga ggtgccggac gctcgagcgc gcgcagccgg gcgccgtcgt ggaatcgaga
     3121 tcaagggacg cgctcgtcgc atcgagcctc ttgatttcga acgcttcgat tacgtgctcg
     3181 cgatggatgc ggcaaacctc gaagatcttc tttcaatcgc acccgaatcg gcgaaggcca
     3241 aggtgcggct gctgcgcagc ttcgatcccg cgtccgctcc aggctcctcc gttcccgatc
     3301 cttactacgg cggcgacgac gggttcgacg aggtgctcga ccaatgcctc gccgcgtgcg
     3361 ctgggctctt gaatcacctc cggcgcgagc actcactgcg atgaggccgg agctagaaag
     3421 ctcgctgcag cggcgcctcg gcgtacacca cctgcgcgcg accgcaatct cgggcggcga
     3481 catcaacgac gcctttcgca tcgattgcgc gaaggggacc ctgttcgtga aggcaaacgc
     3541 ccgcgctccc cgcggtcttt tcgaagcgga ggcgcgaggg ctcgcgtggc tgagcgaagc
     3601 ctccgcgctc cgcatcccga acgtcgtggc ggtatcggat cccgacgagc cggtgagcta
     3661 cttggtgctc gagtacatcg agcccggcag acccgctcga gatttcgacc aggcactcgc
     3721 gcgcgggctc gccaagctgc accgtgcggg cggcgagcgg ctcggtctcg atcacgacaa
     3781 cttcattggg tcgttgccgc aatcgaacgc agccgcgcct tcgtgggccg agttctacgt
     3841 gagccggcgg ctcgcgccgc tgctcgagcg cgccgctgcg accggcaaag cgagctacgc
     3901 gatgcgacgt ggcttcgagc gactcttctc ggtgctacct gggctcgtcg gtcccgaaga
     3961 gccgccgagt cggctacacg gcgatttgtg gagtggcaac ctccacgtag cggaggacgg
     4021 ggcgccctgt ctcatcgatc cggcggtgta cgccgggcac cgagaaatgg atctcgcgat
     4081 gatgcgactg ttcggtggct tttccgagcg cgtcttttcc gcgtatcacg aggctcaccc
     4141 gctcgcgtcc ggagtcgaag aacgcgtcgc gctctaccag ctctacccgt tgatggttca
     4201 cgtgaacttg ttcggtggta gctacgtaac gtccgtggaa cgcgcgcttt cgcgttacgt
     4261 ttgagtcgta cgcggcgcgc gccgggcgag gcgatttgcc gggccaaggc gcctcgctgc
     4321 gtcggaattg tggacgagcg cagtcggcgc tcggcgcccc acttcgctgc atatcgtcgg
     4381 tgaatcgacg cgagtgaatc gcgagtcgtg agcgagtgga cgaccggcac gagagctgca
     4441 cgtggctcgc ggcgcgtttc tttggcgcga gtgaaggaga ggtccatgcg aagagcagcg
     4501 tgtttcgtga tgctcgggtt cgtcgcgtcg gcatgcggag aaggcggatc gaagccttcc
     4561 tcgtcgacgc aaagtgacat ttcggcaccc accaaccgat acccggacgc gccgccgaac
     4621 gcaccggcgc cgcccgcggc gtgcggagac atgaacggtg gcggagatca acacgtcgac
     4681 ttcgctgcgt tgatggcgca gaaggtgagc gagaaacccg aagtcgaagc gcggcaaagc
     4741 acgctcctca tggaccgtta cgacttgagc gacaacccgc acccgacggt tcggatgtcc
     4801 gcgggcaaag cggtgcagcg cggagcccgc gtgaagctcc cgtcgggcgc tagcttcgat
     4861 gcactcgcgt tcatgacgcc agccgagatc cgaagtcagg acgtgttccc gctcgggttt
     4921 tacccgctgc ctcacccgaa gcagcccgag ggcggcatgg tgttcccgca cttccacatc
     4981 gaagaagtgc tccggcagac cggccgcgat ctcacgcgat tcgacctcga tttcgacatc
     5041 ccggacacgt tcttgcccga gttcccgccg cccatctacc tgacgacgca ccccgagctc
     5101 ggggacgtca gccaagggca attggtgacg acggacaact actacgatct cttcaagtgc
     5161 atcctgaatc cgaagcagct cgacgggctg cgcctcttgc tcacgccgtt tccacaacag
     5221 caattcaacc ccatcgacga tcgaaggtcc gcgcggccga gcctgggcgt ggcgtgcttc
     5281 gactgtcacg cgaacggcca caccaatgcc gctacccacc tcgtcggtga cattcgaccg
     5341 caagagctac gtcatcgcat cgacagcgtg tcccttcgtg gcgtgaacat ccagcgacta
     5401 ttcggctcgc agcgtgcgct gatgagtatc gaagacttca ccgagttcga gcagcgcgcg
     5461 gcctacttcg acggcgaccc agtgctcgcc accaagaagg gaatgaacat tttggatcgc
     5521 ggcgcgcagg tgcaaaccat ggcggagttc caaaagatct tggacttccc ccccgcgccg
     5581 aagctcggcc tcgacggcat gctcaaccaa gaagtcgcga ccgcggccga gatccgcgga
     5641 cagaccctgt ttttcggcaa ggctcgctgc tcgacgtgcc acgcgcctcc ctattacacc
     5701 gacctcacga tgcacgatct cgagctcgag cgcttcttcg cgccgaagat gatcaacggc
     5761 atcatggcga acggcgatgg ccccatcaag accttcgcgt tgcgcggtct caaggactcg
     5821 ccgccctatt tgcacgacgg gcgcctcctc accatcgaag acaccgtcga gttcttcaac
     5881 ctagtcctcg ggacgcggct cacgactcag gagaagtcgg atctaaccga gt
//
LOCUS       DASTKS010000690         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_110658, whole genome shotgun sequence.
ACCESSION   DASTKS010000690 DASTKS010000000
VERSION     DASTKS010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110658"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..237)
                     /locus_tag="VFQ35_14210"
     CDS             complement(40..237)
                     /locus_tag="VFQ35_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET9931850.1"
                     /translation="MSRKKISTTVYITPEQNERLHLLHTRTKVPVAVYIREGIDLVLS
                     RYEHQLPGQMSLDVPAKTAKK"
     gene            551..694
                     /locus_tag="VFQ35_14215"
     CDS             551..694
                     /locus_tag="VFQ35_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931851.1"
                     /translation="MPGVSLGGGVESQAEGFFAARRERGARASATDEWVFSKNFSKDF
                     KVT"
     gene            838..1485
                     /locus_tag="VFQ35_14220"
     CDS             838..1485
                     /locus_tag="VFQ35_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931852.1"
                     /translation="MNSSSAERLDANGDGKTDVTIVRDGSREVCRAVDLNFDGVIDSW
                     VYYDGSGQVRRRENDYDRDGRIDEIATYKGGVISEKQRATMLANRIDTWEFYTGGKLA
                     RSERDSDGNQVVDQWWEYPKDGCPIIHADVNSDGQPDPGASVDYCKETGYVPPERQDY
                     RKVESPNFQQGGAALPTEVDSKEVPAQPPSPSSGSASPPAAPAPAQTAPAKGGSK"
     gene            1482..2243
                     /locus_tag="VFQ35_14225"
     CDS             1482..2243
                     /locus_tag="VFQ35_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931853.1"
                     /translation="MKALYWGGLSALVVGLGALLVACGSGGSQAPRTPLKEVAGVPTD
                     DRSRCDFKGRADREVSEATGPGSKFPNIRRVYGIVGQGEERRRVLLCREVDTNLDGAK
                     DIVRTFTDKGEPLSELSDSNYDGKIDTWISFTRGRVAKMQLDTAGRGSADETRFYVGG
                     KLSRAQRDTNQDGKPDVWEVYEDGHLQRIGVDLDFDGHVDRWDRDEIALREIADRERQ
                     ETEAQEKKAQEEKAREQSEAADAGATDARVSARKR"
     gene            2318..>3329
                     /locus_tag="VFQ35_14230"
     CDS             2318..>3329
                     /locus_tag="VFQ35_14230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9931854.1"
                     /translation="MPAERLQKILARAGVASRRAAEELIVNGRVKVDGRVVTELGTRV
                     DARRVRIDLDNKRLVAEPFVYVILHKPRGVMCTLSDPEGRQTVVDLLKGVGVRVVPVG
                     RLDYHTSGALLCTNDGDFAGKLLHPRGGVPKEYVAKVRGLLNDESLARWGESIEIEGR
                     ATRPAEVRFLRHEGDKSWISLVLHEGRNRQVRRLGEATGFPVLRLARVAHAGITTDGL
                     RPGQWRHLTRDELIELKKNYGVPRSIHSADPESLARRPARPEARRSTRKATGPSAPRP
                     SHGSGLRSERGAVTPRASAASGRGGSGRAKATIAHGGAQRSSAHTRASSGRERTSAPN
                     TKQ"
BASE COUNT          601 a         1038 c         1146 g          544 t
ORIGIN      
        1 gtttgagtga gtgcacgcga cgtgagcgtc gtccgagctc tacttcttgg ccgtcttcgc
       61 cggaacgtcg aggctcattt gtccgggaag ctgatgctcg taacggctga gaacgagatc
      121 gatgccctcg cggatgtaca cggcaaccgg caccttggtg cgagtgtgta gcaggtgcag
      181 ccgctcgttc tgctccggcg tgatgtagac cgtggtcgaa atcttcttgc gtgacatcgc
      241 cgctcgcgtg ggctgccgtg cgagccgtta cactagattc ccatacgtgt gttgtcaaat
      301 ctgtccgccg ccacgcaccc aaaaaaacta agtatctttc gatcgttacg gtttttcgga
      361 cgtgagctcc cgagcccgtt tggtggtcgc catgcgttgg tctcgaggcc agttcgggcg
      421 gcgtgtcggg cgggtagagc gctcggatcg gtgaccggtc ggtaggctcc cgggcaggga
      481 cgttactgac agaaatgcgg cgcgctcggt tccatcaaac catgtcgtgt tggccactta
      541 cgtgtgtgac atgcctggtg tttcgctagg gggcggggta gaaagccaag ccgagggctt
      601 ctttgcggct cgtcgcgagc gcggggcccg agcgtcggcc accgacgagt gggtcttcag
      661 caagaacttc agcaaggact tcaaggtcac atgagggtgt ttccgtggat tcgtgccgcg
      721 tctccggccg tgctgttggc ttcattgcag tgtgcttgta gttcgacccc ggcagccaaa
      781 ggggccgccg cctcgtccga gccgcgggat acgcgcattc agcacgaaga gtgtgacgtg
      841 aactcgtcgt cggcggagcg gctcgacgca aacggcgacg gcaagaccga cgtcacgatt
      901 gtacgagatg gttcccgcga ggtgtgtcgc gccgtggact tgaacttcga cggcgtcatc
      961 gattcctggg tctattacga cgggagcggt caggttcgcc gccgcgaaaa cgactacgac
     1021 cgcgacggcc gcatcgacga gatcgcgacc tacaaaggcg gcgtcatcag cgaaaagcag
     1081 cgcgcgacga tgctcgcgaa tcggatcgat acttgggagt tctacacggg agggaagctc
     1141 gcccgcagcg agcgcgactc cgacggcaat caggtcgtcg accagtggtg ggaatacccg
     1201 aaagacggct gccccatcat ccacgccgac gtgaattccg atggccagcc cgaccccggg
     1261 gcgagcgtcg actactgcaa agagacgggc tacgtcccgc cggagcgcca ggactaccgc
     1321 aaggtggaga gcccgaattt ccagcaaggt ggcgcggccc taccgaccga ggtcgattcg
     1381 aaagaggtgc ccgcgcagcc gcctagtcct tcttcgggca gtgcttcgcc gcctgccgct
     1441 cccgcccctg cccagacggc tccggccaaa ggaggctcga agtgaaggcg ctctactggg
     1501 gtgggctctc ggctctggtc gtcggcctgg gcgcgctcct cgtcgcttgc ggttcgggcg
     1561 gctctcaggc gccgcgcact ccactcaagg aggtcgcggg cgttcccacc gacgatcgct
     1621 cccgttgcga cttcaagggg cgggcggatc gcgaagtttc ggaggcgacg gggcctggct
     1681 cgaaattccc caacattcgt cgcgtttacg gcatcgtcgg gcaaggcgag gaacgtcgcc
     1741 gcgtgctctt gtgtcgcgag gtcgatacga acctcgacgg cgcgaaagac atcgttcgca
     1801 ccttcactga caagggcgag ccgctgagcg agctctcgga ttcgaattac gacggcaaga
     1861 tcgacacctg gatctcgttc acccgagggc gcgtcgcaaa gatgcagctc gacaccgcag
     1921 ggcggggcag cgcagacgag acccgcttct acgtgggcgg caagctctca cgcgcgcagc
     1981 gcgacaccaa ccaggacggc aagcccgacg tctgggaagt ctacgaagat gggcacttgc
     2041 aacgcatcgg cgtcgacttg gacttcgacg ggcacgtcga ccgctgggat cgcgacgaaa
     2101 tcgcgttgcg cgagatcgcc gatcgagagc gtcaagagac cgaagcccag gagaaaaagg
     2161 ctcaggaaga gaaagcgcgc gagcagagcg aggccgccga cgccggcgcg accgacgcgc
     2221 gcgtgagcgc tcgcaagcgc tgaatcgacc gccgcccgca aaaatactgg gctctcggtc
     2281 gccggagggg cggcgctgca ctataggtag cgcccccatg ccagccgaac gactgcagaa
     2341 gattctcgcg cgcgccggtg tcgcgtcgcg ccgcgccgcc gaagagttga tcgtcaacgg
     2401 gcgggtgaaa gtcgacgggc gcgtggtcac cgagctcggg acgcgcgtcg acgcgcgtcg
     2461 ggtccgcatc gatctcgaca acaagcgcct ggttgcggag ccgttcgtct acgtgatctt
     2521 gcacaagccg cgtggcgtga tgtgcacctt gagcgatccc gaaggccgcc aaacagtggt
     2581 cgatctgttg aaaggcgttg gggtgcgggt cgtgccggtg ggccgcctcg actaccacac
     2641 gagcggcgcg ctgctctgca ccaacgacgg cgactttgcc ggaaagctgc tccacccgcg
     2701 tggcggcgtg cccaaagaat acgtggccaa agtgcgcggt ctcctcaacg acgagtcgct
     2761 cgcgcgctgg ggagagagca tcgagatcga gggccgcgcg acgcgcccgg cggaggtgcg
     2821 ctttcttcgc cacgagggcg acaagagctg gatttcgctc gtcttgcacg aggggcgcaa
     2881 ccggcaggtg cgccgactgg gtgaggcgac cggctttcca gtgttgcgcc tcgcgcgcgt
     2941 ggcccacgcc gggattacta ccgacgggtt acgtccgggg cagtggcgcc acctcacgcg
     3001 cgacgagctc atcgagttga agaagaacta cggcgttccg cgcagcatcc acagcgcgga
     3061 tccagagtca ctcgcgaggc gtccggcgcg gcccgaggcg cggcgttcga cgcgtaaagc
     3121 cactgggcca tcggcgccgc gcccttcaca cggctccggc ctgcgcagcg agcgcggagc
     3181 ggtcacgccg cgagcgagcg cggcaagtgg tcgcggcggc tcggggcgcg cgaaggcaac
     3241 gattgcgcac gggggcgcgc agcgctcgtc ggcgcacacc cgtgcgagct cgggtcgcga
     3301 aaggacgagc gccccgaaca cgaagcaag
//
LOCUS       DASTKS010000691         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1450314, whole genome shotgun sequence.
ACCESSION   DASTKS010000691 DASTKS010000000
VERSION     DASTKS010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450314"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFQ35_14235"
     CDS             <1..252
                     /locus_tag="VFQ35_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931855.1"
                     /translation="LLANNSSLYNTLKTVTVKECSGKALVVPNDAANSSLIKLTKWEC
                     MDGSNGPFVMPQGCIDDPCLNAQELATLTGWIAAGAPGP"
     gene            complement(253..1125)
                     /locus_tag="VFQ35_14240"
     CDS             complement(253..1125)
                     /locus_tag="VFQ35_14240"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9931856.1"
                     /translation="MKQRSRKAERAALERQRQKDRAFRDDAGRVHLTMHRDPVTGRER
                     VGLKSRIFDSDWQNELVVSAANTVLAALGQRPTLERVVDVTRRAMASTSELIAGLLAR
                     APAGALACKAGCDHCCYQVVGVTAAEVLTIFEHLQRTRSAEERERIRARARQSYERGR
                     GLSSAERFSPEHPCVFLEGGACSIYEVRPLSCRGMNSLDAAECEARLRDPAEWASFLA
                     KGHGGRSYVEPIQAFRAISAGLQLGLAELYRLDVRPLDLTAAVHALFESGASLGSAWI
                     SGQKPFESALRTSG"
     gene            complement(1139..2656)
                     /locus_tag="VFQ35_14245"
     CDS             complement(1139..2656)
                     /locus_tag="VFQ35_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9931857.1"
                     /translation="MQGRPGSLRMPPVLWALLCVAWLPSCSGGSNGGNSSGTGGQSAQ
                     GGRAASSGGSTSGGANGVSSGGAAQGDQTGSGGTSAASAGGIPSAGSAAITGGAPNGG
                     ATLGAGRGGSGDSGAAAGGRANTGGQGGASHSDAFPTFTKHTIASFSSGYATVAVDVD
                     HDGLLDVVALSSGSAGLVWFKNPSWKKYTITTKAKQLIYSAPHDIDGDGHVDLAFISD
                     FDMNNSKSGGTISWAEAPDDPTQSQDWALHPIDAVPTSHRLRWADIDGDGKKELIVLP
                     IFGIGSTQPTRAGAVQLKAYAVPQDPKGAWTGKVLDDTHLEVAHGIEIVDWDGDKAED
                     ILTAANDGIDLFRPSLGSAPQHIGAGKEGQPPDRGSSEVGLGTLGGMRFIASIEFWHG
                     TDAVVYTPGASATELWTRQVLGTDFEHGHGLMVADFDGDGYDEIVAGGGQGDMAEIIY
                     RYAPSTKTWQKIPLDTGSVAVSEIDVKDIDGDGALDIVAIGGSPTNNVVWYENSR"
     gene            2827..>3119
                     /locus_tag="VFQ35_14250"
     CDS             2827..>3119
                     /locus_tag="VFQ35_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931858.1"
                     /translation="MSPPHLSGSPAIPGLDALAMPSSLLSSRWSRDNLITLHPAQLYD
                     GAEHERIEAETLEHLLEELTRERGPFQVESARLGVFTWDVVCQREHDSFVLQVP"
BASE COUNT          526 a         1084 c          950 g          559 t
ORIGIN      
        1 ctactcgcca acaactcgtc gctctacaat acactcaaga ccgtcaccgt taaggagtgc
       61 agcggcaagg cgctggtcgt gccgaacgac gcagccaaca gctcgctcat caagctgacc
      121 aagtgggagt gcatggacgg ctccaacggg ccgttcgtca tgccccaagg ctgcatcgac
      181 gacccttgct tgaacgctca agagctcgcg accctgaccg gatggatcgc ggccggcgcg
      241 cccgggccgt agtcagcccg aagtgcgcag cgccgattcg aagggctttt gcccgctgat
      301 ccaagccgag ccaagcgacg caccactctc gaacagcgcg tgcacggcgg cggtcaaatc
      361 caacggcctc acgtcgagcc gataaagctc cgcaaggccg agctggagcc ccgcgctgat
      421 ggctcgaaac gcctggatcg gttcgacgta gctccgccca ccgtggcctt tggcgaggaa
      481 ggaggcccac tcggctggat cgcgtagacg ggcttcgcac tcggccgcgt cgagcgagtt
      541 catcccccgg cacgagagtg ggcgcacctc gtatatcgag cacgctccgc cttctaggaa
      601 aacgcagggg tgctccggcg agaagcgctc cgcggacgac agcccacgac ctcgctcgta
      661 actctgacgc gcgcgggccc gaatccgctc gcgctcctct gcactgcgag tgcgctgcaa
      721 gtgctcgaag atggtgagca cctcggccgc ggtcacgccg acgacctgat agcaacaatg
      781 gtcgcagcca gctttgcagg ccagcgctcc cgccggtgct cttgccagca aacccgcgat
      841 gagctccgac gtcgacgcca tcgcacggcg cgtaacgtcc acgacgcgct cgagggttgg
      901 cctttgcccg agcgccgcaa gcacggtgtt cgctgcggaa acgaccagct cgttttgcca
      961 atccgagtcg aagatgcggc tcttcaaccc gactcgttcg cgaccggtga cgggatcgcg
     1021 atgcatggtc aggtgaacgc gccccgcgtc gtcacgaaac gcgcggtcct tttgacgttg
     1081 ccgctccagt gcggcgcgct cggctttgcg acttcgttgc ttcacgacct cgcagaactt
     1141 agcgcgaatt ttcgtaccac acgacgttgt tcgtggggct cccaccgatc gcgacgatgt
     1201 cgagcgcgcc gtctccgtcg atgtctttca cgtcgatctc cgagacggca acgcttccgg
     1261 tgtcgagcgg gatcttttgc caagtcttcg tgctcggcgc gtagcggtag ataatttcgg
     1321 ccatgtcgcc ttgcccgccc cccgcgacga tctcgtcgta accgtcgccg tcgaagtcag
     1381 cgaccatgag tccatgacca tgttcgaaat cggtgccgag cacctgccgc gtccaaagct
     1441 cggtcgccga cgcgcccggt gtgtacacca ccgcatcggt gccgtgccaa aactcgatgc
     1501 tggcaatgaa gcgcatccct ccgagggttc cgagcccgac ctcgctcgac cctcgatcag
     1561 gcggctgacc ttccttgccc gcgccgatgt gctgaggcgc gctcccgagt gaggggcgaa
     1621 agaggtcgat cccgtcattg gcggcggtca agatgtcctc ggccttgtcg ccgtcccaat
     1681 cgacgatctc gattccgtgc gccacttcga ggtgcgtgtc gtccaacacc ttgcccgtcc
     1741 acgcgccctt cggatcttga ggcaccgcgt aggccttcag ctgcacggcg cccgctcgcg
     1801 tcggttgagt cgagccgatc ccgaagatcg gcagcacgat gagctccttc ttaccgtcgc
     1861 cgtcgatgtc ggcccagcgt agccggtgcg acgtcggaac ggcgtcgatt gggtgaagcg
     1921 cccagtcctg gctctgggtt gggtcgtcgg gcgcctcggc ccacgaaatg gttccgccgc
     1981 tcttcgagtt gttcatgtcg aagtcgctga tgaacgcgag gtcgacgtgc ccgtctccgt
     2041 cgatgtcgtg cggggcgctg tagatgagct gctttgcttt ggtggtgatc gtgtacttct
     2101 tccacgacgg gttcttgaac cagacgagcc ccgccgagcc cgatgacaac gcgacgacgt
     2161 cgagcaagcc gtcgtgatcc acgtcgaccg cgacggtcgc atagccggac gagaagctcg
     2221 cgatggtgtg cttggtgaac gtcgggaacg catcgctgtg cgaagcgccg ccctgcccgc
     2281 ccgtgttcgc gcgaccaccg gcagcggctc cggaatctcc gctgccaccg cgaccggcgc
     2341 ccagtgtggc gccgccgttt ggagccccgc cagtaatagc cgcagagccg gcgctcggta
     2401 ttccacctgc gctcgccgcg ctcgtcccgc cgctcccggt ttggtcaccc tgagccgctc
     2461 caccggagct cacgccgttg gcgccgccgc tggttgagcc gccgctcgag gcggcgcgac
     2521 cgccttgtgc actctgaccg ccggtgccgc tcgagttgcc gccattgctc ccacccgagc
     2581 aggagggaag ccacgcgaca caaaggagcg cccagagcac cgggggcatc cgcagcgagc
     2641 ctggtcgtcc ttgcatgaca taaacagatg cccgagctgg gccgcggagt tcattcgcga
     2701 tgctgaagcg gtggcttctt ctcggccggc catcagtttg tagcgccccc acaaaccgag
     2761 ctcgctgaaa atcatttgcg ccccgcgccg acgcacatgg gacttcgcta ttctccgcat
     2821 cacggcatga gcccgcctca tctttccggc agccccgcca ttcccggact cgacgcgctc
     2881 gcgatgccat cgtcgctcct ctccagccgc tggtcgagag acaacctcat cacgctgcac
     2941 cccgcgcaac tctacgacgg cgcggagcac gaacgcatcg aggcagaaac tctcgaacac
     3001 ctgctcgaag agttgacgcg cgagcgcggt ccgttccaag tcgaatcagc gcgtttggga
     3061 gtattcacct gggatgtcgt ctgtcagcgc gagcacgaca gcttcgtact ccaggtgcc
//
LOCUS       DASTKS010000692         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_110980, whole genome shotgun sequence.
ACCESSION   DASTKS010000692 DASTKS010000000
VERSION     DASTKS010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110980"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..825
                     /locus_tag="VFQ35_14255"
     CDS             316..825
                     /locus_tag="VFQ35_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931859.1"
                     /translation="MIDDTALSQFERSHSEGLTSAEILDVFARHSIPLSEATLRKYVQ
                     LGLLPRSVRIGKKGKHQGSQGLYPVGVVRQILTIKQMMAESYTIEQIQREFLFIRSDV
                     EQLEQLLEGIFKKLDEVVRERRREHTAHSVTRDVGDARSLSKDLLARLSAIETKLTSR
                     TRMETVAAS"
     gene            1046..1726
                     /locus_tag="VFQ35_14260"
     CDS             1046..1726
                     /locus_tag="VFQ35_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9931860.1"
                     /translation="MDSNNSHGPNDSKSTWHPTEGALALDTETDEHITREQRRSRRKR
                     DVRARTISVKRMTKRELEIGRLLFPETDYWKPRTRAECAEGPRPCPFVSCKHHLYIDV
                     SPRTGAIKLNFPDLEVWDLGESCALDVADRGGTTLEDVGAIMNLTRERIRQVEVKALA
                     KLEALRDMMALRDYVDEGPVGRRRLPRLSREELSDIDNEDEDEVEIESEASTKPAVEA
                     ALPVALDA"
     gene            complement(1840..2901)
                     /locus_tag="VFQ35_14265"
     CDS             complement(1840..2901)
                     /locus_tag="VFQ35_14265"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9931861.1"
                     /translation="MIPARDADGWSRELGISREAVELYLASEVVDLHLDSFIWKRLFG
                     YDLARRHARPPLAGWFLGHADFPRVREAGVASATWVITTNPLRDAPDRTRTLFDNLTE
                     LKRQFAALSEELCFTRTHAEYVAARHAGKHAAFIGIQGGNALDGSADAVERLAPLDVL
                     RVTLVHLSNSSIGQTSSPLRAGADGGLSRFGRELVERLEHARIFVDLAHISRRGFWDA
                     VEVHDRTLPLLVTHTGVSSVHRHWRNLDDSQIRAIAASGGVIGIMYHSAFLGPSLARG
                     RAEWVVDHIEHVVRLVGEDYVALGSDFDGAIVPPRNLTSILELPRLVELMLGRGFGSD
                     VIRKVLGQNFLRVLAQVRP"
     gene            complement(2898..3209)
                     /locus_tag="VFQ35_14270"
     CDS             complement(2898..3209)
                     /locus_tag="VFQ35_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931862.1"
                     /translation="MSDRRDLREATARVLDPDDVEGVLSSAFYTKSPEPREPKTVAHA
                     EDKPQHYKVICISMYTEDLKRLDNMVDALKTRGLTKANRSALIRYALAAVDLDRVPKG
                     L"
     gene            complement(3206..>4544)
                     /locus_tag="VFQ35_14275"
     CDS             complement(3206..>4544)
                     /locus_tag="VFQ35_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9931863.1"
                     /translation="HRLKHFDNDRMETTMAASTPTCSVCSVKFELHFSYQMEQVPGEN
                     GTEERFYCSQRCLEASHHGRSDGQVACDACAKRFAVELAAQVLFTGGRRHYACSSTCR
                     SRILEGVRQMRVGQLMDPSYPLAEENAPGLEDVMPEVTPPPPQATPAPPQVQAARSAP
                     RASTQNAQAVPIMQQPASRPQQRSQRVLAVFNHKGGTGKTTTAVHVAAGLAAKGKRVL
                     LIDTDGQGNVATSLGMKFERSLYHVIVMGLDYREVLQEARPNLHVVPANETVAAAELY
                     LAGQRSRDRLLASRFQGIREAYDYVVVDCSPSLSLMNQNALVMADAVLCPVACDYLSL
                     VGIQKVVRTIKQINRLLSHPLNFWGVLPTFFDTRARICHEALDTLRGHFREVCLEPIR
                     LASRVKEAPSQGKTLFEYAPGSSAAVDYQRVVDRLLGTQQAESKEPVALEGVA"
BASE COUNT          814 a         1416 c         1529 g          785 t
ORIGIN      
        1 gggacgaatt tgcgaggctc attggactcg cgcggctcgc cgccccgcgc ggtgctgcgt
       61 gaccgcgggt tttgccgtgt agggaacgcg gccatggatg gtttatgagc tccatggatg
      121 atgcgtgaga tctgacgttc aatgtcacac ctcgggcacg tgaaggcatg cctggcgcgt
      181 cgcgagcatt tccgcaaatt tttgagggag aagtcctttg ggcgatccat tgagtggaat
      241 atattcgcgc acactgggtg atcacggaag gctcggcgcg agccttcccc tcgtgaaagc
      301 ccgcgagttt ctagcgtgat tgacgacacc gctctcagcc agttcgaacg gagccactcc
      361 gaggggctca cttcggccga gattttggac gttttcgcgc gtcacagcat cccgctgagc
      421 gaggccacgc tgcgcaagta cgtgcagctc gggcttttgc cgcgcagcgt gcgcatcggc
      481 aagaagggca agcaccaggg ctcgcagggc ctctaccccg tgggcgtcgt tcgccagatc
      541 ctcaccatca agcagatgat ggccgagagc tacaccatcg agcagattca gcgagagttc
      601 ctgttcatcc gcagtgacgt ggaacagctg gagcagctcc tcgaggggat cttcaagaag
      661 ctcgacgagg tggtgcgcga gcgccgccgc gagcacaccg cgcacagcgt gactcgcgat
      721 gtcggcgatg cgcgctcgct gagcaaagac ttattggctc gactatcggc catcgaaacg
      781 aaattgacta gcagaacgag gatggagacg gtcgcggcgt cgtaggcact ggcctaggca
      841 ccgtgacgag ctccgctcgg gagctcagcc aagcagcaag ggaagtacga gcgcgaagcg
      901 gtcgggttcg aacagcgggt ctgtcgaatc aataggagac cagggcttct agctcgcagc
      961 taaacagccg tcgaatgtgc cgggccgggt tccgggcgca gctctggcgt gcgtacgcga
     1021 ccattggaga cagcggaggc agggtatgga ttcgaacaac tctcacggcc cgaacgacag
     1081 caagtccacg tggcacccca ccgagggcgc gctcgctctc gacaccgaga cggacgagca
     1141 catcactcgc gagcagcgtc gctcacggcg caagcgcgac gtgcgtgccc ggaccatcag
     1201 cgtcaagcgc atgaccaagc gcgagctcga gattggtcgg ctcttgtttc cggagaccga
     1261 ctactggaag ccgcgcacgc gcgccgagtg cgcggagggg ccgcggccgt gtccgttcgt
     1321 ctcttgcaag catcacctgt acatcgacgt ctcgccgcgc accggagcga tcaagctgaa
     1381 tttccccgat ctcgaggtgt gggatctggg cgagtcctgt gcgctcgacg tcgctgatcg
     1441 cggcggcacg acgctcgaag acgtcggagc gatcatgaat ctcacgcgcg aacgtatccg
     1501 tcaggtcgaa gtgaaggcgc tcgccaagct cgaagcgctt cgcgacatga tggcgctccg
     1561 cgactacgtc gacgagggcc ccgtgggtcg ccgccgtttg ccgcgcctct cgcgcgaaga
     1621 gctttcggac atcgacaacg aagacgaaga cgaagtggaa atcgagagcg aagcgagcac
     1681 gaaacctgcc gtcgaagcgg cgctccccgt cgcgctcgac gcttgaacgg aaaagggcgc
     1741 gaccgagagt tactccgcct ctctcgtcgc gcccgcgcac ggactccgca atcggtgtcg
     1801 ccgtgcgctc gcgaactttc gtgagtgagt cgcttcgact cagggacgca cttgcgcgag
     1861 cacccgcaag aagttctgtc ccagcacttt gcggatgacg tcggagccga agccgcgtcc
     1921 gagcatgagc tcaaccaaac gagggagctc caggatgctc gtcagattgc gcggcggaac
     1981 gatggcgccg tcgaagtcgc tgccgagcgc gacgtagtcc tccccgacga ggcggacgac
     2041 gtgctcgatg tgatcgacca cccactcggc gcggccccgc gcgagcgatg ggcccaagaa
     2101 cgcggagtgg tacatgatgc cgatcactcc gccgctcgcg gcgatggcgc ggatctgtga
     2161 gtcgtccagg ttacgccaat ggcggtgcac ggagctcacg cccgtgtgcg tcacgagcaa
     2221 cgggagcgtt cgatcgtgaa cctcgaccgc gtcccaaaat ccgcgccggc tgatgtgcgc
     2281 caggtcgacg aagatgcgcg cgtgctcgag gcgctcgacg agctcgcggc cgaagcgact
     2341 caggcctccg tcggccccgg cgcgcagcgg agagctcgtc tgaccgatgc tcgagttcga
     2401 caggtgcacg agggtgaccc gcagcacgtc gagcggggcc agtcgttcga ccgcgtccgc
     2461 ggacccatcg agcgcgttcc cgccttggat tccgatgaac gccgcgtgct ttcccgcgtg
     2521 gcgcgcggcg acgtactcgg cgtgagtgcg cgtgaagcag agctcttcgg acaacgcggc
     2581 gaactgccgc ttgagctcgg tcaaattgtc gaacagggtt cgggttcggt ccggggcgtc
     2641 acgcagcggg ttcgtcgtga tgacccaggt cgcggaggcg acgccggcct cccgaacccg
     2701 tggaaagtcg gcgtgaccga ggaaccaacc ggcgagcggc gggcgcgcgt ggcgccgcgc
     2761 gaggtcgtac ccgaacaggc gcttccagat gaagctgtcg aggtgcaggt cgacgacctc
     2821 gctcgcgagg tagagctcga ccgcctcgcg cgagatgccg agctcgcgtg accaaccatc
     2881 ggcatctcga gcggggatca cagccccttc ggaacgcgat ccagatccac cgcggcgagc
     2941 gcgtagcgga tgagcgcgct ccggttggct ttggtgagac cgcgcgtctt cagcgcgtcc
     3001 accatgttgt cgagccgctt caagtcctcg gtgtacatcg agatgcagat caccttgtag
     3061 tgctgcggct tgtcttcggc gtgcgcgacc gtcttcggtt cgcgcggttc gggggacttc
     3121 gtataaaacg cggagctcag cacgccctcg acgtcgtcgg ggtcgagcac gcgggccgtt
     3181 gcttcgcgaa ggtctcgtcg gtcgctcatg ctacaccttc cagcgccacc ggctctttgc
     3241 tctctgcctg ttgggtacca agtagccgat ccacgacgcg ctggtaatcc accgcagcgc
     3301 tcgaaccggg cgcatattcg aatagggtct tgccctgtga gggcgcctct ttcacgcggc
     3361 tcgccaagcg aatcggctcg agacaaacct cgcggaagtg gccgcgcagc gtgtcgagcg
     3421 cctcgtgaca aatgcgggcg cgggtatcga agaacgtcgg caacacgccc cagaagttga
     3481 gtgggtgcga gagcagccga ttgatctgct tgatggtccg caccactttt tggatgccga
     3541 ccaaggagag gtagtcgcac gcgactggac agagcacggc gtcggccatg accagagcgt
     3601 tctggttcat gagagagagg ctcggggagc agtcgaccac cacgtagtcg taggcctcgc
     3661 gaataccttg aaagcggctg gccaagagcc ggtcgcgcga gcgctgcccg gcgaggtaga
     3721 gctccgcggc ggcgaccgtc tcgttcgcgg gcacgacgtg caggttggga cgcgcttctt
     3781 gcagcacttc gcggtagtcg agccccatca cgatgacgtg atagagagag cgctcgaact
     3841 tcatgccgag cgacgtcgcg acgttgcctt ggccgtcggt gtcgatgagg agcacgcgct
     3901 tgccctttgc ggcgaggccg gcggcaacgt ggacggcggt cgtcgtttta ccggtaccac
     3961 ccttgtggtt gaacacggcg agcacgcgct gggagcgctg ctgggggcgg ctcgccggct
     4021 gctgcatgat cggcacggcc tgcgcgtttt gagtgctcgc ccgcggcgcg gagcgggcgg
     4081 cttgaacttg cggcggcgcg ggcgtcgctt gcggtggcgg cggcgtgacc tccggcatca
     4141 cgtcttcgag ccccggcgcg ttctcttccg ccaacggata cgaaggatcc atcagttggc
     4201 ccacacgcat ctgccgcact ccctcgagga tgcgggagcg gcacgtggag ctgcaggcgt
     4261 agtgccggcg acctcccgtg aacagcacct gcgcggcaag ttcgaccgcg aagcgtttgg
     4321 cacatgcatc gcatgcaacc tgcccgtcgg agcggccatg atggctcgcc tcgaggcagc
     4381 gctgagagca gtaaaaccgc tcttccgtgc cgttttcgcc tgggacttgc tccatctggt
     4441 agctgaaatg gagctcgaac ttcacgctgc acacgctgca ggtcggggtg cttgccgcca
     4501 tcgtggtttc catcctgtcg ttatcgaagt gcttaagacg atgg
//
LOCUS       DASTKS010000693         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1227069, whole genome shotgun sequence.
ACCESSION   DASTKS010000693 DASTKS010000000
VERSION     DASTKS010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227069"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2218)
                     /locus_tag="VFQ35_14280"
     CDS             complement(<1..2218)
                     /locus_tag="VFQ35_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET9931864.1"
                     /translation="MLRFLPLGLLAFLPACSASEALSDSSRPSDDRETVASAQAALTV
                     DLTPTGASVIDQRNTVDGEWSIDNLVDGNLSTKSFFHHQDNWIRYQLPGAAVVTRYEI
                     SRSADDYPDRVPTAWTLEASNDGLAWTELDRRDNETLGAPSSYTISNTTPFVYYRLYV
                     RDNHTTANGDTELAEFRIFGEAPTGALPGIVSSVTASVNSDAITVSWSAASNAGSYIV
                     QRVGDDGKSVIEIPTSGLSYTDTKLAPGTTYVYQIQAVNGGRRGPSSTARAVATTAFA
                     ARGLKDLTALSAVAPTDEHATTTGPESVAMLTDNNPYTKWYQGDGSTWVQQQAPAGSV
                     VTEYTLTSANDYPERDPSSWILEASNDGTNWTQIDSRANQGFLARHQTRIFTCNPNKT
                     AYAQYRLSITSTRSSDTQLAEWRLLGTASATLAAPAAPSSLALNTFSYHQDPDDDTSP
                     LVTGNVLTSNQIGFKFADNAGKLNPEASYVLERSTSSSFSSVVSKTVGPNSTAARAIG
                     LSPNTTYWFRIKAVNAAGSSAYSAPLSVTTPAQNAPPNPWQEGGWYGHKSRSLILSDV
                     DDTAKVRVWFDNLVPNPTSIKWLTPVMAAVWKRVTDNYGSLSDPYLNVVGNADGPGYG
                     GGGAGNLYDPRTGFRNVPWVAVSGWSSIDWNDWDNVWKVHAITHETAHIVEGVSVATG
                     YAGAPSVWEDSWWAWIFQYDMYKNLPSVFPNVFSRWRDEGLVGRGDYGWYWARDWLI"
     gene            2564..3151
                     /locus_tag="VFQ35_14285"
     CDS             2564..3151
                     /locus_tag="VFQ35_14285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9931865.1"
                     /translation="MRRLSVVPKRDAADVPAGLEGRSDDELMLLSRGGMESAFATLIR
                     RHQARTLRLAVRYLGRESLAADVAQDTFVEIFRALPQYQAHGRFSSYLYRVLLNRCHM
                     TWRTAQAEQRALTVVSENVSEIDEGELLLRERRRDLTVALGKLSEKLRSVVLLRFSAE
                     LSLEEIAETLEIPTGTVKRRLFDAMTKLREGLAGS"
     gene            3148..>3569
                     /locus_tag="VFQ35_14290"
     CDS             3148..>3569
                     /locus_tag="VFQ35_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931866.1"
                     /translation="MSARRNPDAALLSAYLEGEVTASERASIEAELNESAEARRTLAQ
                     LRDVRALLSAPEAELESMDLPSRVRAAVRQPAAGPATLSSGRRARSMMPLWLGGVAAS
                     AALVALFAFSSKQEGNDAEFRAKGSELRVSEGSRWAGI"
BASE COUNT          618 a         1061 c         1232 g          658 t
ORIGIN      
        1 gaatgagcca atcgcgcgcc cagtaccaac cgtagtcgcc gcgaccgacg agcccttcgt
       61 cgcgccaacg cgagaacacg ttcggaaaga cgctcggcaa gttcttgtac atgtcgtact
      121 ggaagatcca tgcccaccag ctgtcctccc acacggacgg agcgccagcg taaccggtag
      181 cgacgctgac gccctcgacg atgtgcgcgg tttcgtgggt gatggcgtgc accttccaca
      241 cgttgtccca gtcgttccaa tcgatgctgg accagccgct caccgcgacc caaggcacgt
      301 tgcggaaacc ggtgcgcgga tcatagaggt tgccggcgcc accaccaccg tagcccggtc
      361 cgtccgcgtt gccgactacg ttcaggtacg gatcgctcaa gctgccgtag ttgtcggtca
      421 cgcgcttcca caccgcggcc attacggggg tgagccactt gatgctggta gggttcggaa
      481 ccaggttgtc gaaccagacc cggactttgg ccgtgtcgtc gacgtcactc aagatcagcg
      541 agcgcgactt gtgcccatac cagccgccct cttgccaggg gttcggcggc gcattctgag
      601 cgggggtggt gacgctgagc ggcgccgagt acgccgaaga gcccgcggcg ttcacggcct
      661 tgatgcggaa ccaatacgtc gtgttcggcg agagcccgat cgcccgcgcg gcggtgctgt
      721 tcgggccgac cgtcttcgag acgacactcg aaaacgagct actggtcgag cgctcgagca
      781 cgtaggacgc ttctgggttc agcttgccgg cgttgtccgc aaatttgaaa ccgatttggt
      841 tgctggtgag gacattgccg gtaacgagcg gcgacgtatc gtcgtcggga tcttgatggt
      901 aggagaacgt gttgagggcg agggagctcg gagccgcggg cgcggctagc gtcgcactag
      961 cggtgccgag caaacgccac tctgcgagtt gggtgtcgga cgagcgcgtc gacgtgatgg
     1021 agagccggta ctgcgcatag gcagtcttgt tggggttgca cgtgaagatg cgcgtctggt
     1081 ggcgcgccaa gaagccctga ttcgcgcggc tgtcgatttg ggtccagttg gtgccatcgt
     1141 tggatgcctc gaggatccag ctgctcgggt cacgctcggg gtaatcgttg gcggaggtga
     1201 gggtgtactc ggtcaccacg gagccggcgg gagcctgctg ctgaacccaa gtactcccgt
     1261 cgccttggta ccacttggtg taggggttgt tgtcggtcag catcgcgacg ctctcgggcc
     1321 cggtggtcgt cgcgtgctcg tccgtcggag cgaccgcgct gagcgccgtg agatctttca
     1381 agccgcgcgc cgcgaacgcg gtggtcgcga ccgcacgagc cgtggatgag ggtccgcgcc
     1441 ggccgccgtt cacggcttgg atttggtaaa cgtacgtggt cccaggcgcg agcttcgtgt
     1501 cggtgtagct gagcccgctg gtcgggattt cgatcaccga cttcccgtcg tccccaacgc
     1561 gctgcacgat gtaactgccg gcgttgctcg cggcgctcca gctcacggtg atggcgtcgg
     1621 agttcacgct cgcggtgacg ctcgaaacga tgccgggcag agcacccgtg ggtgcctcgc
     1681 cgaagatgcg aaactcggcg agctcggtgt cgccatttgc ggtggtgtgg ttgtcgcgaa
     1741 cgtacaaccg ataatagacg aacggagtcg tgttcgagat cgtgtaactc gacggcgccc
     1801 cgagcgtctc attgtcgcgc cggtcgagct cggtccaagc caggccgtcg ttcgaagctt
     1861 cgagcgtcca tgccgtgggc acccggtcgg gatagtcgtc tgccgaacgg ctgatttcgt
     1921 aacgggtaac caccgctgca cccggcaact ggtaacgaat ccagttgtct tggtggtgga
     1981 agaacgactt cgtcgaaagg ttaccgtcga cgaggttgtc gatgctccac tctccatcca
     2041 cggtattacg ctgatcgatc acgcttgccc cagtgggggt caagtcgacg gtgagcgcgg
     2101 cctgcgctga agcgacggtt tcacggtcgt cactcggtcg agaagaatca gagagcgctt
     2161 cagacgccga gcacgcaggc aagaaagcga gcaggccgag cggaaggaac cgaagcattg
     2221 gttttgcgaa cgatgaagaa tgcatcgttc actcaatgcg tcgacgacgc gctgggttcg
     2281 gtgctgcgct gaagatttcg cgaacagcac gtgcacagac gagcttgcgc cgagttcgtt
     2341 ggcgaacaca cgcgcacgat gcggcgcgcc gcccgccccg gcgtggccgc cggttggttt
     2401 tacactctga gccgagggac gccccgaatg gtcgggcgga cgggcgcgct cggaccgggc
     2461 ccgcaacgct cgggatttct gagcagctag tgctagatgg tccctcgcgt gcacgcgacc
     2521 gaacctttcg gcggtgtggc gcattgtcgt ttcgatggag aacgtgcgtc gattgtcagt
     2581 ggtcccaaag cgcgacgcgg cggacgtgcc cgccgggctc gagggccgca gcgacgacga
     2641 gctcatgctg ctgtcccgtg gcgggatgga atcggcgttt gcgacgctga ttcggcgtca
     2701 tcaggcgcga acgctgcgcc ttgccgtgcg ctatctcggg cgcgagtcgc tcgccgcaga
     2761 cgtggcgcaa gacaccttcg tcgagatatt tagggctctt ccccaatacc aggcgcacgg
     2821 ccgattctcg tcgtacctgt accgcgtgct cttgaaccgc tgccacatga cgtggcgaac
     2881 ggcgcaggcg gaacaacgag cgctaacggt agtgagcgag aatgtttcgg aaatcgacga
     2941 gggggagctg ctactgcggg agcgacgtcg cgatttgacg gtagcgctcg gcaagctttc
     3001 ggagaagctt cgcagcgtcg tgctgctgcg cttcagcgcc gagctcagct tggaggagat
     3061 cgcagaaacc ttggagattc caacgggaac ggtgaagcga cgcttgttcg acgcgatgac
     3121 gaaacttcgg gaagggctcg ctggctcatg agcgcacgac gtaaccccga cgccgcgctg
     3181 ctcagcgcat acctggaagg tgaggtcacg gcgtccgaac gcgccagcat cgaggcggag
     3241 ctgaatgagt cggcggaagc gcgacgcacg ctggcccaac tgcgagacgt gcgcgcgctg
     3301 ctatcggcgc ccgaggccga gctcgagagc atggaccttc cgtcgcgggt acgggcagcg
     3361 gtgcggcaac ccgccgccgg cccagcgacg ttgtcgtcgg gacggcgcgc ccgctcgatg
     3421 atgcccctct ggctcggggg tgtcgcggcg agcgcggcgt tggttgcgct gtttgcgttc
     3481 agctccaagc aggaggggaa cgacgccgag tttcgcgcga aaggaagtga gcttcgcgtt
     3541 tcggagggga gccgatgggc gggcattca
//
LOCUS       DASTKS010000694         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1897382, whole genome shotgun sequence.
ACCESSION   DASTKS010000694 DASTKS010000000
VERSION     DASTKS010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897382"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..252)
                     /locus_tag="VFQ35_14295"
     CDS             complement(70..252)
                     /locus_tag="VFQ35_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931867.1"
                     /translation="MCLICIDFDRGALKVREARRALGEMRAALSAEHVREIEQRLAEA
                     EREAESDGSGNGQPKP"
     gene            complement(292..1056)
                     /locus_tag="VFQ35_14300"
     CDS             complement(292..1056)
                     /locus_tag="VFQ35_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931868.1"
                     /translation="MTNSSHARPVLLVTGGSRGIGAEVARLAARRGYRVALSFLRNEE
                     AAQTVVADVREAGSEACAVQADVSVEADVVRLFEEVDRRFGALRVLVNNAGIVASSER
                     VDAFSAERVARVFAVNVIGSILCAREAVKRMSTRYAGAGGAIINVSSAASRLGSPGEY
                     VDYAASKGAIDTFTIGLAKEVAHERIRVNAVRPGVIDTEIHASGGQPDRAARLAATLP
                     FGRVGTPREVAEAVLWLASEESSYSSGALLDVAGAR"
     gene            complement(1073..2032)
                     /locus_tag="VFQ35_14305"
     CDS             complement(1073..2032)
                     /locus_tag="VFQ35_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9931869.1"
                     /translation="MNDEFDAILAAPSASTTRAPAPPSSAESTVEAPVTHAQIAVESP
                     RFSEFVENWALYRDPILCAVLAGAGLSALGLFVVLRRAVFVTATLTQAAGLGVALAFF
                     VEIHYGLAVPPVLGALISSVLFTALAGARPAKRLSRETAIGFTFVAASALAVLVGDCI
                     TQEAHDIAAILFGTAVLVRPLDLTLVAVTTALSLGALFWLARALTFTGFDPEGAKIQG
                     LPVRALEFAFWSLFAIEVSVATRALGSLPVFAFSVVPAAAGLVLANQLRAAMALSIVF
                     GMLAAAAGYLFAFVFSLPVGASQAAVAALFLLLARAFSRLRTS"
     gene            complement(2029..2766)
                     /locus_tag="VFQ35_14310"
     CDS             complement(2029..2766)
                     /locus_tag="VFQ35_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET9931870.1"
                     /translation="MSARDVGETLVSCSNVVVGYRGAALLPPISLTLRRGEIWAVIGR
                     NGSGKTSFIRTLLSLNAPLSGSVSRERPLRISYLAQRHAYDDLYPLLARDVVGMGLEQ
                     RETFWRKASRDRNVRIEKALTLCGAAELAPRPFRELSEGQKQRVLFARVAVGEPDLAL
                     LDEPTSAMDMVAEQEAFELISRLARELSLCIVLVSHYLGVVRKFADRALLVDRELGRV
                     VVGTPAEVLAHDAFRARYGVVGEGAVL"
     gene            complement(2763..3668)
                     /locus_tag="VFQ35_14315"
     CDS             complement(2763..3668)
                     /locus_tag="VFQ35_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET9931871.1"
                     /translation="MNSLLSKLLFVVSLLILPSRAYAELRVVTSTPDLAAIASAVGGS
                     RVKVKALALPTQDPHWVDARPNLALELSKADLLLAVGAELEIGWLPTLQVGSRNGKVQ
                     KGARGYLECAELVDLLDRPTAKVDRSMGDVHPTGNPHYMLDPRQVEKVAVGVARRMAE
                     LDPEGKGAYLEQATHFVSDLRAARAKWEERLKASRGRQIIAFHRSLVYLADWLGLVVV
                     DEVEARPGVPPNPSHVAEVISRAKQRSVRAVVQESYYPTNTSQLIAQKIGAKLVQLPG
                     GSNFQSGQSYLAFMNQVVNALERSL"
BASE COUNT          676 a         1231 c         1252 g          557 t
ORIGIN      
        1 ctgaaacgca tgcaccccgt cgttcttaca ccgagggatc gagctgcatc ctgcgatccc
       61 gttcagcctt catggctttg gctgtccgtt gccgctgcca tcggactctg cttctcgctc
      121 tgcttcggcg agccgctgct cgatttcacg gacgtgctcc gcgctcagag ctgcgcgcat
      181 ttctccgagc gctcggcgcg cctcgcgcac cttgagcgcg ccgcgatcga agtcgataca
      241 aatcaagcac atgggccgaa gcctactccc ctcctcgcca tggcgcaaaa ctcagcgtgc
      301 gccggcgacg tcgagcaagg cgccgctcga gtaggacgat tcctccgagg cgagccagag
      361 aacggcctcg gccacttcgc gaggagtgcc gacgcgcccg aatgggagcg tcgccgcgag
      421 gcgggcggca cggtcgggct ggccgccgct cgcgtggatt tcggtgtcga tgacgcccgg
      481 tcggaccgcg ttcacgcgga tgcgctcgtg ggctacttct ttggcgagcc cgatggtgaa
      541 ggtatcgatg gcgcctttcg acgcggcgta gtcgacgtat tcacccggcg agcccagccg
      601 cgaagcggcc gacgaaacat tgatgatcgc accgccggcg ccggcgtagc gcgtggacat
      661 gcgctttacg gcttcgcggg cgcacaagat gctgccgatc acgttcaccg cgaacacgcg
      721 tgcgacgcgc tcggcgctga acgcatcgac gcgctccgaa gaggcgacga tcccggcgtt
      781 gttcacgagc acgcggagcg cgccgaagcg ccgatcgact tcctcgaaca gcctgacgac
      841 gtcggcctcg acgctcacgt cggcctgtac ggcacacgct tcgcttccgg cttcgcggac
      901 gtcggcgacg accgtttgcg ccgcttcttc gttgcgcaag aagctgagcg caacgcggta
      961 acctcttcga gccgcgaggc gcgcgacctc ggcgccaatg ccgcggctac ctccggtgac
     1021 gaggaggacg gggcgggcgt ggctcgaatt ggtcatggta agtcagcgta gatcagctcg
     1081 tgcgcagtcg ggagaacgcg cgcgccaaga gcaggaacag ggccgccacc gctgcttgcg
     1141 aggcgccaac cggcagggaa aaaacgaacg cgaataggta gccggccgcc gccgcgagca
     1201 tgccgaacac gatggacaac gccatcgccg cgcgcagctg attcgcgagc acgaggcctg
     1261 ccgccgcggg aaccaccgaa aaggcgaaca cgggcaagga gccgagcgcg cgtgtcgcga
     1321 cggagacctc gatcgcgaaa agggaccaaa acgcaaactc gagcgctcgc accggtagcc
     1381 cttgaatctt ggcaccttcg gggtcgaagc cggtgaacgt gagcgcgcgg gccagccaaa
     1441 atagtgcgcc gaggctcagc gccgtggtca cggcgacgag cgtgagatcc agcggacgca
     1501 ccaacacggc cgtgccgaac aagatggcgg cgatatcgtg tgcctcttgg gtgatgcagt
     1561 ccccgaccag gactgcgagc gcggacgcgg cgacgaacgt gaacccgatc gcggtttcgc
     1621 gcgagagccg cttggcgggc cgcgctcccg cgagagcggt gaacaggacg ctcgaaatga
     1681 gagcgccgag cacgggaggt accgcgagcc cgtagtggat ctcgacgaag aacgcgagcg
     1741 cgacgccgag ccctgccgcc tgcgtcagcg tggccgtgac gaacactgcg cggcggagca
     1801 cgacgaagag cccgagcgcc gagaggcccg cgcccgcgag caccgcgcaa agaatcgggt
     1861 cacggtagag cgcccagttc tccacgaact cgctgaagcg cggcgactcg accgcgattt
     1921 gcgcgtgggt cacgggtgcc tcgaccgtgc tctccgcgct actcggagga gcgggcgctc
     1981 gcgtcgtgct cgcgctcggt gccgccaaga tcgcgtcgaa ctcgtcgttc acaatacggc
     2041 tccttcgccg acgacgccgt agcgcgcgcg aaatgcgtcg tgggcgagca cctccgcggg
     2101 cgtaccgacg acgaccctcc cgagctcgcg atcgacgagc agcgcgcggt ccgcgaactt
     2161 gcgcacgacg ccgaggtaat ggctgacgag cacgatgcac agcgagagct ctcgtgcgag
     2221 ccgagaaatc agctcgaagg cttcttgttc ggcgaccatg tccatcgccg acgtcggctc
     2281 gtcgagcagg gccaaatccg gctcgcccac ggcgacccgc gcaaagagca cacgctgctt
     2341 ttgcccctcg gagagctcgc gaaaggggcg cggcgcgagc tcggcggcgc cgcacagggt
     2401 gagcgccttc tcgatgcgga cgttacggtc gcgcgaagcc ttgcgccaaa aagtctctcg
     2461 ttgctcgagg cccatcccga cgacgtcgcg cgcgagcaac gggtacaagt cgtcgtaggc
     2521 gtgacgctgc gccaaatacg agatgcgaag cgggcgctcc cggctcaccg aaccggagag
     2581 cggcgcgttc agcgacagca gcgtccggat gaagctagtc ttcccgctgc cgttgcggcc
     2641 aatgacagcc caaatctcgc cgcgccggag cgtcagcgag atcggcggca ggagcgcggc
     2701 gccgcgatag cccacgacca cgttggagca cgagaccagt gtttcgccga cgtcgcgagc
     2761 gctcacaacg accgctccag cgcgttcacg acctgattca tgaacgcgag gtaggactgt
     2821 cccgactgga aattgctccc tcccggcagt tgcacgagct tcgctccgat tttttgtgcg
     2881 atgagctgcg acgtgttcgt ggggtagtag ctctcttgaa cgacggcgcg cacgctgcgt
     2941 tgcttggcgc gcgagatgac ctcggcgacg tgcgaagggt tcggtggaac cccggggcgc
     3001 gcctcgactt cgtccacgac gacgagcccg agccagtccg cgaggtacac gagcgagcgg
     3061 tgaaacgcga tgatctggcg tccacgcgac gccttcagcc gctcttccca cttggcgcgc
     3121 gcggcgcgca aatcgctcac gaagtgagtc gcttgctcca ggtacgcacc cttgccttcg
     3181 gggtcgagct cggccatgcg ccgcgcgacc ccgacggcca ctttctcgac ctggcgagga
     3241 tcgagcatgt agtgcgggtt accggtggga tgcacgtcgc ccatcgagcg atctaccttt
     3301 gcggtgggac gatccaagag gtcgaccagc tcggcgcatt cgaggtagcc gcgggctccc
     3361 ttttgcactt tgccgttgcg cgagccgacc tgcagcgtcg gaagccagcc aatttcgagc
     3421 tcggcgccga ctgccaacaa gagatcggcc ttgctgagct cgagcgccaa attgggtcgc
     3481 gcatccaccc agtgcggatc ctgggtgggt agcgccaagg ccttcacttt gacgcgagag
     3541 ccccccaccg cgctcgcgat ggcggcgagg tcgggcgtgg aggtgacgac gcgtagctcg
     3601 gcgtaggcgc gcgacggcag gatcaacaaa gagacaacga agagcagctt cgacaagaga
     3661 gagttcatgg acagctccga tcagaaaggg tgtgcgccgt gcgcgccggc cgcgaa
//
LOCUS       DASTKS010000695         5125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1002544, whole genome shotgun sequence.
ACCESSION   DASTKS010000695 DASTKS010000000
VERSION     DASTKS010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002544"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFQ35_14320"
     CDS             complement(<1..369)
                     /locus_tag="VFQ35_14320"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9931872.1"
                     /translation="MVARRRLLLLVALLSAACDLSRPLGEPLARGEDQSRGRPAKTIA
                     LNGAGATFPYPLYSKWMSEYNRLNPEVRINYQSIGSGAGIAQVKAGTVDFGATDKPLA
                     SDDLAQSNLAQFPTIIGGIVP"
     gene            complement(486..1820)
                     /locus_tag="VFQ35_14325"
     CDS             complement(486..1820)
                     /locus_tag="VFQ35_14325"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET9931873.1"
                     /translation="MHKLLGSLTGLALALVGVPALAQAPAPAAPAAAPAAPSPPTPGA
                     DLGTPAAAAPTAPVEVAPPASPAAPPPALAAPAASPTPAAPTPPAEAKPAPVGPEKLA
                     IGKALQGYFQPSLLLQFWGFYSHNPNITTGDKNASTFRVRRAEVRVKGDIIPNLLSYN
                     LMFDPAKAFAYGSKNLPVTGQVPAPDTAGQVAAQQPSYTDPAILQDYFITLKSDYADV
                     SLGQFKIPVSYEGFNGASKIIFPERALVARQYGDRRELGVKVEKKLGDYFFYSLGIFN
                     GSGVNTTDNDNEKDVALRLEAYPIKGLTVAGVGYRTFGERDGGYRDRLEADVRYEGYD
                     VLVLGEYIHAWDGPKGKEIEGHGAYGTLGYTFLKSIQPVARFGFLNPNKDLFGDRVRH
                     YEGGINYYLQALEARATLAVSAFELQDKKAANGTLSPQKTRWEAILAFQAGF"
     gene            complement(2397..3098)
                     /locus_tag="VFQ35_14330"
     CDS             complement(2397..3098)
                     /locus_tag="VFQ35_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931874.1"
                     /translation="MAKILVVEDEPDLQQVLEYNLKQAGHEVLATKLGQEGIRLAREH
                     RPDIVLLDLMLPDSSGIEVCRALKEGATTKGIPVLMVTARGEEIDRVVGFEIGADDYV
                     TKPFSVRELLLRVQAVLRRGKPEPEGAETIVDFGRLRIDRGAHRVWVDSQEIELTALE
                     FKLLVTLYDRRNRVQTRSALLDDVWGIQADITTRTVDTHVKRLREKLEGARDYVETVR
                     GVGYRFVGTPDEAVS"
     gene            complement(3224..4420)
                     /locus_tag="VFQ35_14335"
     CDS             complement(3224..4420)
                     /locus_tag="VFQ35_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9931875.1"
                     /translation="MHALLAITLVLSALSLVLTAATHFAVRLHLKRAPKPLAAAPPIS
                     VLKPLKGIDDELYANLVSLARQDYPSFELVLGCEDPGDPALSLAYRLQQEFPHVPIRV
                     LYGADARGFNPKVNNVRMLSRGARHDWILISDSSVRARRDYLRAMATELEDGVGLVSS
                     VLVGAGEQNLGARLDNMQMNSFVVRGVCGADALVAHPCVVGKSMLFRKSALESLGGFE
                     RVENVLAEDYVLGQMFRDAGYRVVLSSHLLASVSSRRSLREFCSRQVRWSQMRRHLAP
                     GMYFAELLQSPIPWLLTALALVVSGATTNGRALWATSIAAGLTLRLASDGWLMRELRG
                     APLAVRDYAAIVLKDLIWVWIWMQGGVRREVTWRGNSLRIGRGSALTPVELEPERARE
                     AVGSLS"
     gene            complement(4520..>5125)
                     /locus_tag="VFQ35_14340"
     CDS             complement(4520..>5125)
                     /locus_tag="VFQ35_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9931876.1"
                     /translation="GKVGLALAREQRPDLVLLDLMLPDVSGTEVCRAIKDDPSTRAMP
                     VVVLTARNSEIDRVVGFELGADDYVTKPFSIRELLLRIRAVLRRSESRPVEANLVDFG
                     TLRVDRDAHRVWVDGGEVELTLLEFKLLVTLYDRRNRVQSRGSLLDHVWGMDTKISTR
                     TVDAHVKRLREKLGSARDYIETVRGVGYRFAEIPSLGGGGW"
BASE COUNT          935 a         1694 c         1642 g          854 t
ORIGIN      
        1 cgggacgatg ccgccgataa ttgtcggaaa ctgcgcaagg ttgctttgcg cgagatcgtc
       61 ggaagcgagc ggcttgtcgg tcgcgccgaa gtcgacggta cccgctttca cctgggctat
      121 tccggcgccc gagccaatcg actgataatt gatgcgcacc tcgggattga ggcggttgta
      181 ctcggacatc cacttcgaat agagcggata cgggaaggtc gcgcccgcgc cgttcaacgc
      241 gatggtcttc gctggacgcc cacgcgattg gtcctcgccg cgcgcgagcg gctcaccgag
      301 cggacgcgag aggtcgcacg ccgccgaaag caacgcgacc aagagcaaga ggcgccgtct
      361 cgcgaccacg atcgttccct aacagcggcc aggaaatcgg gcgggacggc gccgcaacag
      421 atgagtgaca gttccgtgat tggtgcgccg cggcgaaggt cccggccttg ctcgcgatca
      481 cgcaatcaga agccagcctg aaaggcgaga atcgcttccc agcgggtttt ctgcgggctc
      541 agcgtcccgt tggccgcctt cttgtcctgg agctcgaacg ccgaaacggc gagggtcgcc
      601 cgcgcttcga gcgcctgcaa gtagtagttg atgccgcctt cgtagtggcg aacgcgatca
      661 ccgaacaagt ccttgttcgg gttcaagaag ccgaagcggg ccaccggctg aatgctcttg
      721 aggaacgtgt acccgagcgt gccgtacgcg ccatgtcctt cgatctcctt gcccttcggc
      781 ccgtcccaag cgtggatgta ctcgccgagc acgagcacgt cgtaaccttc gtagcggacg
      841 tcggcttcga gacggtcacg gtagcctcca tcgcgctccc cgaaggtgcg gtatccaacg
      901 ccagcgacgg tgaggccctt gatcgggtac gcctccaagc gcaaggcgac gtccttctcg
      961 ttgtcgttgt cggtggtgtt cacgcccgag ccgttgaaga tcccgagcga gtagaagaag
     1021 tagtcgccga gcttcttctc cactttgacg ccgagctcgc gccggtcgcc gtactggcga
     1081 gccacgagcg ctcgctctgg gaagatgatc ttggaggcgc cgttgaatcc ctcgtagctc
     1141 actgggatct tgaattgacc gagcgacacg tcggcgtaat ccgacttcaa agtgatgaag
     1201 tagtcctgca ggatggccgg gtccgtgtag ctcggctgtt gcgcagcaac ttgccccgcc
     1261 gtatccgggg ccggcacctg accggtgacg gggaggttct tcgagccgta ggcgaaggcc
     1321 ttggctgggt cgaacatcaa attgtacgaa agcaggttcg gaatgatgtc gcccttcact
     1381 cgaacttctg cgcgacgcac gcggaacgtc gaagcgttct tgtcgccggt agtgatgttc
     1441 ggattgtgcg aataaaagcc ccagaactgc agcaataggc tcggctgaaa gtacccctgc
     1501 agcgcctttc caatcgcaag cttttctggg ccgacgggtg cgggtttggc ctcggcgggc
     1561 ggagtgggcg cagcgggtgt cggcgaggcc gcgggcgcgg cgagcgccgg cggaggcgcg
     1621 gcgggcgagg ctggcggagc gacctcaacc ggcgcggtcg gggccgcggc agcgggggtg
     1681 cctaagtcag cgccgggcgt gggtggtgac ggtgcagcgg gtgcagcggc aggagcggcg
     1741 ggggcaggtg cctgtgcgag cgcgggcacc cccacgaggg ctaacgctag tcctgtcagt
     1801 gaaccgagaa gcttgtgcat gtatttcgtt ccttctaggt cgcgaccaaa cacgtcgaac
     1861 gtgtcggtat gaagacgact gcgtgacgtt caattcacac gttcgtacga ctgtctcata
     1921 taagagacac gcgaataacg caagagacgc gcgcggaagg ctcgaccggc caaccgcgcg
     1981 acgcctccgc cctacggccc ggttgccgcg tttttcaggc actttggcag gtcggccgct
     2041 ggccgtagag ctccgtcgcg cgccgacggc cgacggccga cgaccaacga ccaacgacca
     2101 acgaccgcaa gatggacacg acgcccgcga cccgaggcgg ctggaatgga cccgagcgcg
     2161 agccccaacc cacgctcgcc actcgaagcc gaggccgcct tcacgaccca ggttcacgcg
     2221 ccttcacggc ccaggttcac gcgccttcac ggcccaggtt cacgcgcctt cacggcccag
     2281 gttcacgcgc cttcacggcc caggttcacg cgccgcgcgg cgacaaggtt ccaccaccca
     2341 cgacccagcc ggctgcggtc acgctcgcgc gcagccggct atggctttcc gccaactcag
     2401 ctaacggctt cgtccggcgt ccccacgaaa cggtacccga ccccgcgcac ggtctcgacg
     2461 tagtcgcgcg cgccctcgag cttctcgcgg aggcgcttca cgtgggtatc gacggtgcgg
     2521 gtcgttatat cggcctgaat gccccaaacg tcgtcgagga gcgcgctccg cgtttggacg
     2581 cggtttcgcc gatcgtaaag ggtcacgagg agcttgaact cgagcgccgt gagctcgatc
     2641 tcttgcgagt ccacccaaac tcggtgtgca cccctatcga tacggagcct tccaaagtcg
     2701 acgatcgtct ctgctccttc gggctcgggc ttaccacggc gcagcaccgc ctgaacgcga
     2761 agcaagagct cgcgcacgct gaacggcttc gtgacgtagt cgtcggcgcc aatctcgaaa
     2821 ccgacaactc gatcgatctc ttcgccgcgc gccgtaacca tcagaaccgg aatgcctttg
     2881 gtggtcgccc cttctttgag tgctcggcac acctcgatcc ccgaactgtc ggggagcatc
     2941 agatccagca gtacgatgtc gggccggtgc tcgcgcgcca aacgaatgcc ttcttgcccg
     3001 agcttcgtgg ctagcacctc gtgcccagcc tgcttcaggt tgtactcgag gacctgctgg
     3061 agatccggct cgtcctcgac gactaagatt tttgccattc cggtcctcat catgcgtcac
     3121 atctcgccat ccgtcttccg ggtagcggtc acgtgtgaca cctgggtgac gcaagtgtag
     3181 tagaagcgtg ccagtcgcgc cggctcgctg cgccgcggag ccttcaggaa aggctcccga
     3241 cggcctcccg agcgcgctcc ggttccaatt ccacgggcgt caacgccgaa ccacggccga
     3301 tgcgcaggga gttgccgcgc cacgtcacct cacgcctgac cccgccctgc atccaaatcc
     3361 aaacccagat gaggtccttc aagacgatgg ccgcgtagtc gcgcaccgcg agaggcgccc
     3421 cccggagctc gcgcatcaac cagccgtcgg acgcgagccg cagcgtaagc cccgccgcga
     3481 tgctggttgc ccagagagcg cggccgttcg tggtcgcgcc cgaaaccacc agcgcgagcg
     3541 ccgtcaggag ccaaggaatc gggctctgca gcagctcggc gaagtacatg ccgggcgcga
     3601 ggtgccgccg catctggctc cagcgcactt gacgtgagca gaactctcgc aagctgcgcc
     3661 gcgagctcac cgacgcgagc aggtgcgacg agagcacgac ccggtagccc gcgtcgcgga
     3721 acatctgtcc gagcacgtag tcttcggcca gcacgttctc gacgcgctcg aagccgccga
     3781 gtgactcgag cgcgctcttc cggaagagca tggatttacc gaccacgcac gggtgcgcca
     3841 cgagcgcgtc ggcgccgcat acgccgcgca cgacgaacga gttcatctgc atgttgtcga
     3901 ggcgagcgcc gaggttctgc tcgccggctc cgaccaggac gctcgagacg agcccgacgc
     3961 cgtcttccag ctccgtcgcc atggcccgga ggtagtcgcg ccgcgcgcgt acgctcgagt
     4021 cggatatgag aatccaatcg tggcgagcgc cgcgactcaa catccgtacg ttgttgacct
     4081 tcgggttgaa gccgcgcgcg tcggcgccgt agagcacgcg aatcgggacg tgcgggaact
     4141 cttgctgcaa gcggtacgcg agtgacaacg cggggtcccc agggtcttca cagccgagca
     4201 ccaactcgaa gctcgggtag tcttgacgag cgagcgacac tagattcgcg tagagctcgt
     4261 cgtcgatacc cttcagcggc ttcaggacgg agatgggagg cgcggctgcg agcggcttcg
     4321 gcgcacgctt gaggtgcaac cggacggcga agtgcgtcgc ggcggtgagc acgagtgaca
     4381 gcgccgacaa aaccagagtg atcgcgagca gagcgtgcat ggactctgcc gatccacact
     4441 cggtgagctc ggtgaaagcc ccctgacgag agggcaacgc ttgggcaact tcagccccga
     4501 acgagcgcgg cgccgcgtct taccagccac cgccaccgag cgacgggatc tctgcgaagc
     4561 gataaccgac accgcgcacc gtctcgatgt agtcacgagc ggagccgagc ttctcccgca
     4621 agcgcttcac gtgcgcgtcg accgtgcgcg tcgaaatctt ggtatccatg ccccacacgt
     4681 gatccaggag cgacccacgc gactgcacgc ggttccggcg atcgtacagc gtaacgagca
     4741 gcttgaactc gagcagcgtg agctcgacct ctccgccgtc gacccacacg cggtgcgcgt
     4801 cgcggtcaac gcgcaacgtc ccgaagtcca ccaaattggc ctcgaccggg cgagactcgc
     4861 tgcggcgaag caccgcgcga atgcgcagca gtagctcgcg gatgctgaag ggcttggtca
     4921 cgtaatcgtc cgccccgagc tcgaaaccga ccacgcgatc gatctcgctg ttgcgcgcgg
     4981 tgagcaccac gaccggcatc gcgcgggtgg atgggtcgtc cttgatggcg cgacacactt
     5041 cggtgcccga tacgtccggc aacatcaaat cgagcaggac caagtcgggg cgttgttcgc
     5101 gagcgagcgc gaggcccacc ttccc
//
LOCUS       DASTKS010000696         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_334121, whole genome shotgun sequence.
ACCESSION   DASTKS010000696 DASTKS010000000
VERSION     DASTKS010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334121"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1680)
                     /locus_tag="VFQ35_14345"
     CDS             complement(1..1680)
                     /locus_tag="VFQ35_14345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931877.1"
                     /translation="MANRTALKPGDTLGRFQLLVPIGAGGMGRVWVAREQGLTARPRL
                     VAIKTALAEEAASEDYWKVLLDEARIASQIQHPNVCAIHALDRERGVVYLVMDWSDGG
                     SLREVLDATGENRLPVHIAARLVAHVCAGLQAAHDLVGDDGVALGVVHRDVSPQNILL
                     SSNGQVKLTDFGVAKARGQIHAPTQTGEVKGKLSYMAPEQVTTRDVDRRADVFALGCV
                     LYEATVGERPFHGDDALSTLYQLLERPVQAPTSKRLDYPVGLERIVLKSLERDPEARY
                     QTAEEMGRALQMWLASERTMVTDSDMAELVRVTLGDRIAARAKAVEEALVVVDSPEID
                     ADEPEQEAPAKPGVETLSGSAASTLPGNQLPRAPRKTLWAGVASAAVALSLVAAFAGN
                     RGSQQPLPNPVASATPAPLAAPAPVPAKEAEPARAEDVTITLRVSPDNASLTLDDGPP
                     LPNPYTIRVAPDARQHHVRAHLAGYEDHEETVVFDQSRQLSFALSKQTGGAPRGRAPA
                     VRVASVTNPTPPADTPAAPTQPTAPRNLGELPPVVKKPPRALDAENPFAKP"
     gene            complement(2243..2845)
                     /locus_tag="VFQ35_14350"
     CDS             complement(2243..2845)
                     /locus_tag="VFQ35_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931878.1"
                     /translation="MLAIAAASIVLALLGFWSFSAGIAFLLIGCALAASLAPRGFAFY
                     AFAGLAPSFILALRDGARSGPFLPLLVACAAAALGGRLLADSRDRSASPDVAARLRRL
                     ALLIWGLTLFPLYPAAKLSWVRVQVRRFCDDVHPGDAVTGLEARARSLGLEVVNRPAP
                     LDGSRKPALSAFQGFTFARTFCDVEYEAGRVTDARRTFVD"
     gene            complement(2912..>3747)
                     /locus_tag="VFQ35_14355"
     CDS             complement(2912..>3747)
                     /locus_tag="VFQ35_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET9931879.1"
                     /translation="AILSEWREDSEVGRINARAGEWVEVGPETMAVLEKSLWIGRASE
                     GTFDITFQAMSGVWRFGSAQSEHPTLPSPAEVRKLRALIDYRKVELDPKSSRVRIPKG
                     RKIGLGGIAKGYIVDRAVEVLRRAGLTAFLVQAGGDLYGAGRKPDGSPWVSGIQDPRG
                     PRGAFFATIELENHAFSTAGDYERAFVLDGKRYHHIIDPRTGYPATASRSVTVHAPDA
                     TTADMIDDAVFILGPEKGLKLAESLPGVGVVIVDAHNKVWVSPTLQGKVRLQSSPTDG
                     L"
BASE COUNT          616 a         1170 c         1333 g          628 t
ORIGIN      
        1 ttagggcttg gcgaaggggt tttcggcgtc gagagctcgc ggcggcttct tcacgaccgg
       61 gggaagttcg cccaaattgc gtggggcagt gggttgcgtc ggcgcggccg gggtgtcggc
      121 aggcggagtc gggttggtca cgctggcgac gcgcacggct ggtgctcggc cgcgcggcgc
      181 gccaccggtt tgcttgctga gggcgaagga gagttgtcgc gattgatcga agacgacggt
      241 ctcttcgtgg tcctcgtagc cggcgaggtg cgcgcgcacg tggtgctggc gggcgtcggg
      301 agcgacgcga atcgtgtacg gattcggcaa cgggggaccg tcgtcgagcg tgagcgaggc
      361 gttgtccggc gagacgcgga gggtgatggt tacgtcttcg gcgcgcgccg gctcggcctc
      421 tttcgcaggc acgggtgctg gcgcggcgag cggcgcgggc gttgccgaag cgaccgggtt
      481 cggcagtggt tgctgcgagc cccggttccc ggcgaacgcg gcgacgaggc tgagggcaac
      541 cgcggcactg gcaacaccgg cccatagggt cttgcgaggg gcgcggggca gctggttgcc
      601 aggcaacgtg ctcgctgcgg agccggacag cgtttcgacg ccgggcttgg ccggagcttc
      661 ttgctcgggc tcgtcggcat cgatttcggg cgagtcgacg acgacgaggg cttcttcgac
      721 ggccttggcg cgcgccgcga tgcggtcgcc cagggtcacc cgcacgagct ctgccatgtc
      781 cgagtcggtg accatggtgc gctcggacgc gagccacatc tgcagggcgc gacccatctc
      841 ttcggcggtt tggtaacgag cctcgggatc gcgctcgaga gacttgagca cgatgcgctc
      901 gagccctacc ggatagtcta ggcgcttgct ggttggcgct tgaacggggc gctcgagcag
      961 ctgatacagc gtcgaaagcg cgtcgtcgcc gtggaagggg cgttcgccca cggtcgcttc
     1021 gtagagcacg cagccgagcg cgaacacgtc cgcacgacga tcgacgtcgc gcgtggtgac
     1081 ctgctccggc gccatgtacg agagcttgcc cttgacctcg ccggtctgag tgggggcgtg
     1141 aatttgcccg cgtgctttgg cgacgccgaa gtcggtgagc tttacctgac cgttcgagga
     1201 cagcaggatg ttttggggcg aaacgtcgcg atggacgacg ccgagggcga cgccgtcgtc
     1261 gccgactaga tcgtgcgcgg cttgaaggcc tgcgcagacg tgagcgacga gccgcgccgc
     1321 gatgtgcacc ggcagccggt tctccccggt tgcgtcgagc acctcgcgca gcgagccgcc
     1381 gtccgaccaa tccatgacga ggtagacgac gccgcgctcg cgatcgagcg cgtggatggc
     1441 gcacacgttg ggatgttgaa tctgcgaagc gatgcgcgct tcgtcgagca acactttcca
     1501 gtagtcttcg ctggcggcct cttccgccag cgcggtcttg atcgcgacga ggcggggccg
     1561 tgccgtgagg ccttgctccc gcgcgaccca aacgcgtccc ataccgccag cgccgatcgg
     1621 caccaacaac tgaaagcgac cgagagtgtc gccgggttta agcgctgttc gattcgccac
     1681 gatgcaacat tcgagcttag tccatggcgc tccgtttcgc atctagaact tgccactcaa
     1741 tttgcgatca cacattcata caagcgtgtg tgatgcacag aattgtgtgt gcgagacgga
     1801 gcttgccttc acttattaaa cggctcgact ctctcaccct tcagagcgaa ttcgcaccgg
     1861 aaatcgcgtc gagaagtgag cttctgcacg cgacgcgagg catgaaattt tcgatgtgca
     1921 agggacacga gggctcatcg tttgtagagg tgggacaatg gctgctcgtg tgtgactcga
     1981 ggggcgccgt cggtcggggc aggcggctcg agtcgagacc cccgcctcgc ggctctcggg
     2041 gactctcgac gccgccttcg gcaaagacga tcgcgcgagc cgacgattcg cagtggcccg
     2101 cggcctcgag ttaggaccga ctcgctcgcg aggaggcccc cgagactcgc gtcgcggcga
     2161 gctcgtggcg aaattcgccg agcgatgccg cgttcgttca gacgatgctg cggggccgag
     2221 ctcgcgttgg tcgcgctgcg gttcagtcga cgaaggtgcg acgcgcgtcg gtgacccgcc
     2281 cagcttcgta ctcgacgtca cagaacgtgc gcgcgaaagt gaagccctga aaggcactga
     2341 gcgcaggctt gcgtgagcca tcgagcggcg cgggtcgatt cactacctcg agcccgagag
     2401 agcgcgcgcg tgcttcgagc ccagtgacgg catcgccagg atgcacgtcg tcgcagaagc
     2461 gccggacttg tactcgcacc cagctcagct tcgcggcggg gtagagcgga aaaagggtca
     2521 gcccccagat gagcagggcg agccgccgga ggcgcgcagc gacgtccggc gacgccgagc
     2581 gatcgcggct atccgcgagc agtcgaccac cgagcgcggc cgccgcgcat gcgacgagga
     2641 gcgggaggaa aggccccgat cgcgctccgt cgcgcagcgc gaggatgaag ctcggcgcga
     2701 gccccgcgaa cgcatagaag gcgaagccgc gcggcgcgag cgaagcggcg agcgcgcatc
     2761 cgatcagcaa gaacgcgatg ccggccgaga acgaccaaaa gccgagcagg gcgagcacga
     2821 tggaagccgc cgcgatcgcg agcattcggt tgttttacgc tgcgagagcg cgcccctcaa
     2881 acgcgtcctt cgcggcgccg accgccggaa ttcaaagccc gtcggtcggt gaactctgca
     2941 gtcggacttt gccttggagt gtagggctca cccacacctt gttgtgcgcg tcgacgatga
     3001 cgacaccgac gcccggtaac gattcggcga gcttcaggcc cttttcgggt cccagaatga
     3061 acacggcgtc gtcgatcatg tcggcggtcg tcgcgtcggg cgcgtgcacg gtaacgcttc
     3121 gcgaggcggt cgcggggtaa ccggtgcgtg gatcgatgat gtgatggtag cgcttgccgt
     3181 cgagcacgaa ggcgcgttcg tagtcgcccg cggtgctgaa agcgtggttc tcgagctcga
     3241 tggtggcgaa gaacgcgcca cgcgggccgc gcggatcttg aatgccgctc acccacggcg
     3301 agccgtcggg cttccgcccc gcgccgtaca gatcgccacc ggcctgcact aggaaagcgg
     3361 tgagaccggc gcgccgcaac acctcgacgg cgcggtcgac gatgtagccc ttcgcgatgc
     3421 cgccgaggcc gatcttgcgt cccttcggta tgcgcacgcg ggagctcttg ggatcgagct
     3481 ccaccttgcg ataatcgatg agcgcgcgga gcttgcggac ctctgcaggg gaaggcagcg
     3541 tcggatgctc ggactgcgcg gagccgaagc gccacacgcc gctcattgcc tgaaaggtga
     3601 tgtcgaaggt gccctccgac gcgcgcccga tccagagaga cttctcgagt accgccatcg
     3661 tctctgggcc gacctcgacc cattcgcccg cgcgcgcgtt gatgcggccg acctcgctgt
     3721 cctcgcgcca ctcgctgaga atcgctt
//
LOCUS       DASTKS010000697         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1898007, whole genome shotgun sequence.
ACCESSION   DASTKS010000697 DASTKS010000000
VERSION     DASTKS010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898007"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFQ35_14360"
     CDS             complement(<1..306)
                     /locus_tag="VFQ35_14360"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET9931880.1"
                     /translation="MTDSRAPSAEPRVRVLGAPRPPLRDFYHALLRLSWTRTLLLVAG
                     SYLALNLLFACFYSLAGGVAHAHEGFIDYFYFSVQTMGTIGYGAMFPETHPANLLVVL
                     "
     gene            445..1389
                     /locus_tag="VFQ35_14365"
     CDS             445..1389
                     /locus_tag="VFQ35_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931881.1"
                     /translation="MAKETRKLRRAQEQKEPNKDKDLLKRPMAKPVEIGDPLGPQQFR
                     SLLLRLGLPLLAIWLVCGFIASMVYSETVKVLLIALPVVLTAAAAGLVIWVMRQAKKA
                     RGVASILKNVETQEDRKAAIQQLESSFKKSDPAALFAKAQLELQEDPKKALATLEQID
                     LGKVMAPIADEARSQRAMIHLMIGEVSPARQLVDGIDLSRHQDPRSRAMIASVCAEAW
                     ARSGNAKKAVETLELFDPEESTFEQLRPQIYRAQAFAYAQTNDQTRMRRAMKKLASQD
                     VRLLGGFMVKRTHPLLQKEAKKLLEQSGLPRRMMVQRG"
     gene            complement(1409..>3175)
                     /locus_tag="VFQ35_14370"
     CDS             complement(1409..>3175)
                     /locus_tag="VFQ35_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HET9931882.1"
                     /translation="LKIAAFPSLLLITTLVRLALNVSSTRLILLQANAGEVIRAFGEF
                     VVRGNYVVGGVIFLILTIIQFIVIAKGGERVAEVGARFTLDALPGKQMAIDAELRAGS
                     IDGNEARMRRHKLARESQFYGAMDGAMKFVKGDVLASLLITLVNLFGGLAIGVFQRGL
                     EPTQALKRYGLLTIGDGLVTQIPSLVLATAAGVLVTRVASEDPDTPLGEELAQQLLGQ
                     PQALRVAAFFVLLLALAPGLPAFPFLVIAGLLGFASRARSRALRVEASKAAAEPIQRR
                     ADGNEPRFVPVVVAWGFEVSSDLIPLEDDDLRSEQRRPGLRSAMAAIREIMFRELGVP
                     LPRGRLAENRDLPPRTALLSLYEIPARLIEVPADVNDAQVASFLISASMPLLRQRAAD
                     FFGIAEAQSLLDQLEQVAPATVRQVVPKPVSVPLLADVLRRLLEEHISIRDLRAILEA
                     LSAVAASEKDPLNLAEFVRTQLRRAITHGLTRGARELSVLLVDAPIEETIRGAIQRTP
                     AGSFLALAPAAGRDVVQAVRRALQTVPPGTSAVLLTQPDIRRFMRKLLEVELPELFVA
                     SYAELLPEVALRPVARVTLRDL"
BASE COUNT          594 a          940 c         1158 g          483 t
ORIGIN      
        1 gaggacgacg aggaggtttg cggggtgggt ctccgggaac atcgcgccgt agccgatggt
       61 gcccatcgtt tggacgctga agtagaagta gtcgatgaag ccttcgtgag cgtgcgcgac
      121 gccgcccgcg agtgaataga agcacgcgaa gagcaggttc agcgcgaggt agctgccggc
      181 gacgagcagc agggtgcgcg tccacgagag tcgcaggagc gcgtgataga agtcgcgaag
      241 cggcgggcgc ggcgcaccca agactcgcac tcggggttcg gcgctcgggg cgcgtgaatc
      301 ggtcacgccc aatggtttgg cgggttttca cccgtgcgac aagcggctcg aagcgcgaca
      361 acgcgctagt tttcgtgggt ttaggcgcac tcgcgtgtca ccggcagtat tgagaggccc
      421 gggggaacgc gctatagcga ccgcgtggcc aaagagacgc ggaagctgcg acgagcgcag
      481 gagcaaaaag aaccgaataa agacaaagat ctgctgaagc gcccgatggc caagccggtg
      541 gaaatcggcg acccgctcgg ccctcagcag ttccggagct tgctcttgcg gctgggtctg
      601 ccgctgctcg cgatttggct cgtgtgtggg ttcatcgcca gcatggtcta ttccgagacc
      661 gtgaaggtgc tcttgatcgc gctgccggtg gtgctcacgg cggcggcggc cgggctcgtg
      721 atttgggtga tgcgccaggc caagaaggcg cgcggcgtcg ccagcatctt gaagaacgtc
      781 gagacgcaag aggatcgcaa ggcggcgatt cagcagctcg aaagctcttt caaaaagagc
      841 gatccggcgg cgctgttcgc caaggctcaa ctcgagcttc aggaagatcc gaagaaggcg
      901 ctcgcaacgc tcgagcaaat cgacctcggt aaggtgatgg cgccgatcgc ggacgaagcg
      961 cgctcgcagc gagcgatgat tcatctgatg atcggtgaag tgagccccgc ccgccagctc
     1021 gtggacggca tcgatttgtc gcgccaccaa gaccctcgct cgcgcgcgat gatcgcttcg
     1081 gtgtgcgccg aggcgtgggc tcgcagcggc aacgccaaga aggcagtaga gacgctggag
     1141 ctgttcgacc ccgaagagtc gaccttcgag cagcttcgcc cgcaaattta tcgcgctcag
     1201 gcgttcgcct acgcgcagac caacgatcag actcgcatgc gccgcgcgat gaagaagctc
     1261 gcgagccaag acgtgcgcct gctcggcggg ttcatggtga agcgcacgca ccccctcctg
     1321 caaaaagaag cgaagaagct tctcgagcag agtgggcttc cgcgccgcat gatggttcag
     1381 cgcggctaat cgacagtcga atcccggact agagatcgcg cagggtgacc cgggcgacgg
     1441 ggcgtagtgc gacctcgggg agcaattcgg cgtaggaggc gacgaagagc tccgggagct
     1501 cgacctcgag cagtttgcgc atgaagcggc ggatatcggg ttgggtgagg agcaccgccg
     1561 acgtcccggg cggcaccgtt tgcaaggcgc gccggacggc ttggacgacg tcgcgaccgg
     1621 cggccggtgc gagcgcgagg aagctcccgg ccggagtgcg ctggatggcg ccgcggatgg
     1681 tttcttcgat cggcgcatcg acgagcaaca ccgagagctc tcgcgcgccg cgggtgagcc
     1741 cgtgcgtgat ggcgcgacga agctgggtgc gcacgaactc ggcgaggttc agcggatctt
     1801 tttcggaggc ggcgacggcc gagagcgctt ccaagatcgc gcgaaggtcc cgaatgctga
     1861 tgtgctcttc gagcaggcgc cggaggacgt cggcgagcag cgggacactg accggcttcg
     1921 gaacgacttg gcgcaccgtc gccggcgcaa cttgctcgag ctggtcgagc aggctctgcg
     1981 cctcggcgat gccgaagaag tccgccgcgc gctggcgaag cagcggcatg ctcgcgctga
     2041 tcaggaaaga tgcgacttgc gcgtcgttga cgtcggcggg cacttcgatg aggcgcgccg
     2101 ggatctcgta gagggagagg agcgccgtac gcggcgggag atctcggttc tcggcgaggc
     2161 gcccgcgcgg cagcggcacg ccgagctcgc ggaacatgat ctcgcgaatc gccgccatgg
     2221 cggagcggag gccggggcgg cgctgctcgc tccggaggtc gtcgtcttcg agcggaatga
     2281 gatcggagct gacctcgaag ccccacgcga cgacgacggg cacgaagcgc ggctcgttgc
     2341 cgtcggcgcg gcgttggatt ggctcggcgg cagccttgga ggcctcgacg cgcagggcac
     2401 ggctgcgagc tcgtgacgcg aagccgagca ggcccgcgat gaccaagaac ggaaacgccg
     2461 gcaggccggg cgcgagcgcg agcaacagca cgaaaaacgc ggcaacccgc aacgcctgag
     2521 gctggccgag cagctgctgc gcgagctctt cgccgagcgg tgtatcggga tcttcgctcg
     2581 cgacgcgcgt gacgaggacg cccgcggcgg tggcgagcac gagcgacggg atttgcgtga
     2641 cgagcccgtc gccgatggtg agcaggccgt atcgcttgag agcctgggtc ggctcgagcc
     2701 cgcgttgaaa gacgccaatc gcgagcccgc cgaacaggtt gacgagcgtg atgaggagcg
     2761 aggccagcac gtcgcccttc acgaacttca tggcgccgtc catcgcgccg taaaattggc
     2821 tttcgcgcgc gagcttgtgg cggcgcattc gcgcttcgtt gccgtcgatg ctgccggcgc
     2881 ggagctcggc gtcgatcgcc atttgcttgc cgggcagggc gtcgagcgtg aagcgcgcgc
     2941 cgacctcggc gacgcgctcg ccgcctttgg cgatgacgat gaactgaatg atggtgagga
     3001 tcaggaagat gacgcccccc accacgtagt tgccgcgcac gacgaattcg ccgaaagcgc
     3061 gaatgacttc gcccgcgttg gcctgcagta aaatcagtcg cgtggaagag acgttgagag
     3121 cgaggcgaac gagcgtcgtg atgagcagga ggctcgggaa cgccgcgatt ttcag
//
LOCUS       DASTKS010000698         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1451680, whole genome shotgun sequence.
ACCESSION   DASTKS010000698 DASTKS010000000
VERSION     DASTKS010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451680"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VFQ35_14375"
     CDS             complement(<1..1578)
                     /locus_tag="VFQ35_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9931883.1"
                     /translation="MSRASVGRVLVVDDERLVAREIAEELEEFGYEVAATASTADEAL
                     SLAREKRPDLVLLDVRIRGPRDGIEAATVLRDRFRIPVVFLTACADAETLTRAKSTDP
                     YAYLVKPVSASDLRSTVEIAMNRARIERTLSEQFHWLSTTLRSIADGVVCVDARGCIT
                     FLNPTAERLTGWTLSEAVGKPWDDVVVLLDEDTRSSRRGPLNEALSQRTVAALPEGTL
                     LRSHDGTLYAIQDSAAPILSDDSALTGAVMVFQDVSEKRQLKRQAELNDRLASLGTLA
                     AGVAHEVNNPLSYIIGNQLFAQGELAEIVPLLSSVDPELRNAIQEKLESAVAALRDAS
                     SGAERVRAITSDLKMLGRPNPQRHRTLQPNALLDAALKITISRLSERARVVREFGECP
                     SIRGDEVRLTQVFVNLLLNAAHAIPAGQADQNEIRIVTRTSARGGAEIEIRDTGSGIS
                     KELIPRIFEPFFTTRGLETGTGLGLSISHGIVSSLGGSIEVDSRVGAGSSFRVLLPTA
                     TKVSSFPPPPPRPSLASG"
     gene            complement(1575..2729)
                     /locus_tag="VFQ35_14380"
     CDS             complement(1575..2729)
                     /locus_tag="VFQ35_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HET9931884.1"
                     /translation="MTTDHETSRAESDLENVGFHEEAASRRSRAPASTGTKAASRFHT
                     LIVEDDQAEACLLRKFLEDASQGYPSVGHFSAEWTSTLAAALARLSTGSVDAIVLDLG
                     LPDSQGLATLRMIREAARDAAIVVIASTSELELGLDALGLGAQNMLPKDELGPGTIAR
                     TLRCAIERRRIETELLATIREKEILLRELNHRAKNNLQVVGALLSMQARRSEDEGFRA
                     LVAAARSRIEAMARAHERLRSSNDLSRIDFSFYLRDLASSIYSSHGGESRGIALELDI
                     EQHELSLERAVTAGLIVNELLTNAFKHAFDADREGRIWLRFRGDDKLLELLVEDNGRG
                     MAGAEPGTRAEVGLGLGLVATLAEQLDAALDCDSGPGTRLRLTFPRGDSA"
     gene            complement(3257..3925)
                     /locus_tag="VFQ35_14385"
     CDS             complement(3257..3925)
                     /locus_tag="VFQ35_14385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9931885.1"
                     /translation="MKRLIAVVLVLAVANGLAFRDSLAGSPVFLWTLAVPYLALCGLA
                     FHHFWDEGTLLDRLKPRGGDISIGAVSALVLLFASWLGRSALAPSGTPRQAWLYRIYL
                     QLGDPEILQHSVTLTLLLLFIAAAEEIVWRGLVLALASERFGTRRGWLITTVLYAATI
                     CPTAYFLRDPEAGLNPLLPTAALGAGLVWGFLAGRLGRLMPVVVSHMAFTYFSAVQFR
                     WPMG"
     gene            complement(3922..>4527)
                     /locus_tag="VFQ35_14390"
     CDS             complement(3922..>4527)
                     /locus_tag="VFQ35_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET9931886.1"
                     /translation="TLSNRSSGKMGFAIAERAALHGAKVTLIAGPVALPTPPGVTRID
                     VRSAVAMRSALWLALHPDLSGADALIMAAAVADYRPAETHASKIKRGNGPLVLELVPN
                     PDLLAEIGEARRGLFPVLVGFALETDTDERVVERAREKLAKKRVDMVVANRAEESLGL
                     DYVRATLVSAKDAESLPALPKEDAADKILDFIANRFQEPLR"
BASE COUNT          782 a         1459 c         1507 g          779 t
ORIGIN      
        1 cccgctcgcg agcgatggac gcgggggggg cggcggaaag gagcttacct tcgtagccgt
       61 cggcagcagt acgcgaaagg agctccccgc gccgacgcgg ctgtctactt cgatgctgcc
      121 gccgagcgac gacacgatcc cgtgggaaat cgagagcccg aggccggtcc ccgtctccaa
      181 tccgcgcgtc gtgaagaacg gctcgaagat acgcggaatg agctccttcg agatcccgga
      241 gccggtgtcg cgaatttcga tttccgcgcc gccgcgagcg ctggtccggg tgacgatacg
      301 aatctcgttc tggtcggctt gaccggcggg gatcgcgtgc gccgcgttga gcagtaggtt
      361 cacgaagacc tgcgtgagcc tgacttcgtc tccgcggatc gacggacatt caccgaactc
      421 gcgaacgacg cgcgcacgct cgctcaagcg cgagatcgtg attttgagcg cggcgtcgag
      481 cagcgcgttc ggttggagcg tgcggtgacg ctgcgggttc ggtcggccga gcatcttcag
      541 gtcggaggtg atggcgcgca cccgctccgc gccgctgctc gcgtcgcgca acgccgccac
      601 cgccgactcg agcttttcct ggatggcatt tcggagctcg gggtcgacgc tcgagaggag
      661 cggcacgatc tcggcgagct ctccttgcgc gaacagctga ttgccaatga tgtacgagag
      721 gggattgttc acctcgtgcg ctacgccggc ggccaaggtc ccgagcgacg ccaggcggtc
      781 gttcagctcg gcttgccgtt tcagttgacg cttctcactc acgtcttgaa agaccatcac
      841 cgcgcccgtg agcgcgctgt cgtcgctcag gatcggcgcg gccgaatcct ggatggcgta
      901 gagcgtgccg tcgtggctgc gcagcagcgt cccttccggg agagcggcca cggttcgctg
      961 cgacaacgct tcgttcaacg gtccgcggcg cgaggaacgc gtgtcttcgt cgagcagcac
     1021 cacgacgtcg tcccacggct ttcccaccgc ctccgagagc gtccaacccg tgagtcgttc
     1081 cgcggtcggg ttcaagaaag tgatgcaacc acgcgcgtcg acgcagacca cgccgtcggc
     1141 gatcgaacgc agcgtggtcg atagccagtg aaactgttcg ctcagggtac gctcgatgcg
     1201 agcgcggttc atcgcgatct cgacggtgga gcggaggtcg ctcgcgctca ccggcttcac
     1261 cagatacgca tacggatcgg tcgacttggc gcgcgtcagc gtctcggcgt cggcgcacgc
     1321 ggtgaggaac actaccggga tccgaaagcg gtcgcgcagc acggtcgcgg cctcgattcc
     1381 gtctcgtggg ccccggatac gcacgtcgag cagaacgaga tcggggcgct tctcgcgagc
     1441 gagtgagagc gcttcgtccg cggtcgaagc cgtcgctgca acttcgtaac cgaactcctc
     1501 gagctcttcg gcgatctctc gggccacgag ccgctcgtcg tcgacgacca gcacgcgccc
     1561 caccgacgcc cggctcatgc ggagtctcca cgcggaaacg tcaagcggag ccgcgtaccg
     1621 gggccggaat cgcagtcgag cgccgcgtcc agctgctcgg cgagggtcgc gacgagcccg
     1681 aggcccaagc ccacttccgc tcgcgtccct ggttccgcgc cggccatgcc tcggccgttg
     1741 tcctccacga ggagctcgag gagcttgtcg tcgccgcgga agcgcaacca aatgcgaccc
     1801 tcgcggtccg cgtcgaaggc gtgcttgaag gcgttcgtga ggagctcgtt cacgatgagt
     1861 cccgcggtca cggcgcgctc gagcgagagc tcgtgttgct cgatgtcgag ttcgagcgcg
     1921 atgccgcggc tctctccgcc gtgtgacgaa tagatcgagc ttgcgagatc tctcaagtaa
     1981 aaagaaaagt cgattctcga taggtcgttc gagctgcgga ggcgctcgtg ggcgcgcgcc
     2041 attgcctcga tgcggctgcg cgcggccgcc accagcgcgc gaaagccttc gtcctcgctc
     2101 cgccgcgcct gcatcgacaa gagcgcgccg acgacttgga ggttgttctt cgcgcggtgg
     2161 ttgagctcgc gcaggagaat ctctttctcg cggatggtgg cgagcaactc cgtctcgatg
     2221 cggcgccgct cgatcgcaca tcgcagcgtt cgcgcgatcg tgccgggccc gagctcgtct
     2281 ttgggcagca tgttctgcgc accgagcccg agcgcatcga ggccgagctc gagctcgctg
     2341 gtcgaggcga tgacgacgat ggcggcgtcg cgcgcggcct cccggatcat gcgtagcgtc
     2401 gcgagcccct ggctgtcggg caacccgagg tcgagcacta tcgcatccac gctaccagtg
     2461 gacagccgcg cgagcgccgc cgcgagcgtt gacgtccact ccgcgctgaa gtgaccgacg
     2521 ctcgggtagc cctgcgaagc gtcttccaaa aactttctca gcaggcatgc ttccgcctgg
     2581 tcgtcctcca cgatcagcgt atgaaaccgt gaagccgctt tcgtccctgt cgaggccgga
     2641 gcgcggctac gacgcgacgc cgcttcctcg tgaaagccga cgttttcgag gtcagattcc
     2701 gcgcgcgagg tttcgtgatc cgtagtcatc taggacaatt cgtcgcagcg ctggccgtgc
     2761 tcgctgcgtt caccacccga gcgcttcgtg acggcgaacg tgccgcgacg gtcgcttgct
     2821 gagatatcgc catgaaagga tcgtttcctc ttccgctacg aagggttcgt tccgaggctt
     2881 cagctcgtgt ggtttgggcg ctccacaccg gcgaaaagcg gttggaacgg cgttccgaaa
     2941 agtgacgaac gatgggctca aactatcaca gcagacagcc cctcacatat ggcgagtttc
     3001 gccaggcttt aacgttgggc cacatcgaac caaacacgcg ttcacgggcg gaagacgaag
     3061 gctctgcact gcgcgcgcac gcgcggcgag cggtgtcgtt tggatgaatt tcgacggctg
     3121 tcgggccccg cactgacgcg tgcaacgcca ctcaatgcaa actgggttct cgtggtcgtc
     3181 ggtcgggtga ttgaggccag ctccagtcgc tagagaggcc gcaaacacgg cgcgctgagc
     3241 ccgtgcgtgg ccgcggtcac cccatcggcc agcgaaattg cacagcagag aagtacgtaa
     3301 acgccatgtg cgaaaccacg accggcatga gcctgccgag ccgtccggca aggaagcccc
     3361 acacgagtcc ggccccgaga gcggcggtgg gtagtaatgg attcagtcca gcttcgggat
     3421 ctcgcagaaa gtacgcggtg ggacagatcg tggctgcata gagcaccgtc gtaatgagcc
     3481 atccgcggcg cgtcccgaag cgttcagacg ccaaggccaa caccagcccg cgccagacga
     3541 tttcttcagc agccgcgatg aacaacagca agagcgtcag agtcacgctg tgttgcaaaa
     3601 tctcggggtc gcccagctga aggtagatgc gatagagcca cgcctgtcgt ggggttccgc
     3661 tcggggcgag ggcgctgcgg cccagccacg acgcgaacag cagcacgagc gcgctgaccg
     3721 cgccgatcga gatgtcccct ccgcgcggct tcaatcggtc gagcagggtc ccctcgtccc
     3781 agaagtgatg gaaggcgagg ccgcacaaag ccaagtaagg gacggcgaga gtccacaaga
     3841 atacgggaga gcctgccagc gagtcgcgga acgcgagccc gttcgcgact gcgaggacca
     3901 acaccaccgc gatgaggcgc ttcatcggag cggctcctgg aagcggttcg cgatgaagtc
     3961 gaggattttg tcggcggcgt cttctttcgg caacgcgggc agcgactctg cgtctttcgc
     4021 gctcacgagt gtcgcgcgca cgtagtcgag gccgagcgac tcttcggcgc ggttcgcgac
     4081 gaccatgtcg actcgcttct tcgccaactt ctcacgagcg cgctcaacga cgcgctcgtc
     4141 ggtgtcggtt tcgagtgcga agcccaccag caccggaaac aggccgcgtc gggcctcgcc
     4201 gatctcggcg agcaaatccg ggttcgggac gagttcgagt acgaggggtc cgttgcctcg
     4261 tttgattttc gaggcgtgcg tctcggccgg gcggtaatcg gccacggccg cggccatgat
     4321 caaggcgtct gcgccggaaa ggtccgggtg cagcgcgagc cagagcgcgc ttcgcatcgc
     4381 gaccgcgctg cgtacgtcga tgcgagtgac gccgggtggc gtcggcagcg cgacgggacc
     4441 cgcgatgagc gtcacttttg ctccgtgcag ggccgcccgc tcggcgatgg caaagcccat
     4501 ctttccggac gagcgattgc tgagggt
//
LOCUS       DASTKS010000699         8174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_558253, whole genome shotgun sequence.
ACCESSION   DASTKS010000699 DASTKS010000000
VERSION     DASTKS010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558253"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFQ35_14395"
     CDS             complement(<1..412)
                     /locus_tag="VFQ35_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931887.1"
                     /translation="MRASPAASLLAPFLGALVLAASCGTDATGIEDCRAIEEARCEAA
                     EACGAIDDASACKRFYRDQCLHGLSVPAPGGPVTDACVAAIHQITECARASAAEASTA
                     CARFRNRTQACKLVPSPQNLNECNFLTPGIPPIGE"
     gene            513..1385
                     /locus_tag="VFQ35_14400"
     CDS             513..1385
                     /locus_tag="VFQ35_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET9931888.1"
                     /translation="MVLCAGFGTRLRPLTDELPKPLLPYGDRSLLEHAVRSFQAAGFA
                     EVVANVHHLAERFEAALTGFGGSVALVHEPVLRGTAGGIAGARRALGPSPVLCTIGDV
                     VLERIPTELVDAAGFGGLVLAVAPRVAGSGTVGIGAHGEVVRLRGERFGQEVSGGDYI
                     GLCALGDRALTELPEMGCLVGDYALPVLREGGEVRTFAYRGAYVLPGDDLGSYFASQL
                     AWLAERGLASFVADEAKVSRGVLVDETLIGARATVTGTGVLRRVVALPGATLEAPLES
                     AIVAPSGRIIRIDS"
     gene            1398..2666
                     /locus_tag="VFQ35_14405"
     CDS             1398..2666
                     /locus_tag="VFQ35_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9931889.1"
                     /translation="MLSSNLEVSVVLKVTPLDLRPDAAQIARRLWDREGLVLVRVGAF
                     TFVACDPVEVAERFDPEPTLARRALLHGESPRWFGLLPYESLRGGEGELESDTRAAAL
                     VERPLWLRYGALVRVGDGVELIADSEDAARQLAALLERAPRARDVELSFDGALEAPGL
                     HALRVQKALEEIASGNIYEVNLARRFELSVNGSAPELLAAFERSGKMPHSFALEHRDL
                     KVVAVSPELCLRLEPDGELMTSPIKGTRPRHPDPLEDQRLARELEEDPKERAELTMII
                     DVERNDFGRVATLGSVRLSEPPHVVSLPGVHHRVASVTAQVAPELGRDELFEAVLPSG
                     SVTGAPKRRAMQLIRELEPHRRGLYTGAVGFVRHDGGVELKMAIRTLVARGSRGHYFA
                     GGGIVADSVPEREVEETLWKAERLLSLAAG"
     gene            2767..3615
                     /locus_tag="VFQ35_14410"
     CDS             2767..3615
                     /locus_tag="VFQ35_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9931890.1"
                     /translation="MQPVNVRNLEQIQEHDGVPKSSRLGALLLAALASAAVVTSSVMM
                     SRQKGPPTASSADPLAELVANAKAQAPAERLEGKEVTFPAVLSDREKPTTALAAVKDE
                     RGRLVARDEPEAPAAPTSPPPAADRLPVVPLPIGTLLTATPVTTEPKDRLTSLASDAA
                     RGAESSSLAPAGSDGGFQLQVASFKDQNDADKLVDELRHRGHRAFRQAAYVPERGLWH
                     RVRIGPFKSKLEAQKYKAEFERTERVSPFLVDPEKVKQAEQNRAMRLAAQEKKGGRHI
                     AVPASE"
     gene            complement(3619..4737)
                     /locus_tag="VFQ35_14415"
     CDS             complement(3619..4737)
                     /locus_tag="VFQ35_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931891.1"
                     /translation="MLSDTELGSLPGPAQKALHVDTPLPMKLMAARGVLPGLKPAEIV
                     IVVAALSASEDEKLRDTATATLGKLPPPILNGALSADLPTLVVDRLAHAYTNQHEVVE
                     QLLRMPRLGAAALEHMAEVADERSGELIATNERRMLEYPSVIEKLYMNKRVRMSTADR
                     LIELAVRNGLELNLPAFKEAAQAIKNELVPEPSEEPTYDDELFTNTEQIAKTADVADD
                     EDTHERDEEGEEHLRDKFVPLYAIIGQLTVTQKIRRATLGTAAERLLLVRDANRLVAT
                     AAVKSPLMRENEAAQISASRAVSEEVLRTIALNRDFTRNYQVKLNLVSNPRTPLTYAA
                     RFMPHLRDGDLRALQKSKNVPGAISLAARQQLMRKQGK"
     gene            complement(4832..6001)
                     /locus_tag="VFQ35_14420"
     CDS             complement(4832..6001)
                     /locus_tag="VFQ35_14420"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9931892.1"
                     /translation="MAEFLERVANARERVSPKWTPGRERELRFRVERGLDRRRTTIRA
                     LGGVALAVAALFLGHTGYRQVFGNTHIVAQAPLRHDVRNLFELTDGTRVTALSTDARV
                     EPVEVSREAVRVRLEAGGAHFDVAHVEGRHFQVQARDVLVTVLGTAFSVAVESGGVRV
                     EVERGRVDVGFDGQHRILGAGERIFASEGAFRTGQSAPRSAASAEVPAIEPVPPVEPS
                     SEAPNSNAPSTRRAPAAAPSSWRTLAQDGDYRSAFERMQAEGSNAVRDEPGDLLFAAD
                     VARLSGHAALALPLLERVLRGHPHDSRAPLAAFTLGRTLLDSLGRPREAADAFGKARR
                     LSPNGALAQDALAREVESWSRAGESVVARQRALEYLERYPGGRREKAVRYHGGLE"
     gene            complement(5994..6572)
                     /locus_tag="VFQ35_14425"
     CDS             complement(5994..6572)
                     /locus_tag="VFQ35_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9931893.1"
                     /translation="MQRARVALEIVPPVAPRETGEFDVVAAPEAALDFESVFKRYSPY
                     VAAVAHRLLGRDADVDDTVQEVFMVAVRGLSRVRDPLAVKSWLARIAVRVSRRKLQMR
                     RVRSFFGVDDPAAYDVIADDQASPEQRALLQRVYRVLDSLPADQRIAWTLRHIEGEQL
                     DAVAALSGCSLATAKRRISAAARTLEEAFSDG"
     gene            6861..8135
                     /locus_tag="VFQ35_14430"
     CDS             6861..8135
                     /locus_tag="VFQ35_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9931894.1"
                     /translation="MGLMDFVKGGVGRMMIARPDEHKDKVVYKHPDQTFPFWSQLTVD
                     SDEVCLFFKDGTYVGALPPGRHTLQTQNIPFLNALVDKFTGGNVFISEIYFVTTRPLY
                     NQGFGDTIGSMRDPELDIRVTPRAFGTYSFRVVDPLKFVVGFRGQTGGDPELSLRWVR
                     DQLFMGLKATLSRLIKSGELTLLDLGTCGPDIARAIVQNCPDLANIGVQVLEIAKLNI
                     NLSDEDQHRIDAAQGEIVQAKLAARKARIGIAQAEAEAQARQYQLDQEFLNRARYVNQ
                     LDMGRYQQYAAAEASLGLGAGMAQGGEGAGAAAAAAGVAQGLALGAGLAARPPYPPPG
                     YPAAGYPPPGYPPPGYPVPPAGYPGYPPPGPPPAPAVAAAPAAYAPPVPPPPVAPAAA
                     SGSDAQVRCASCNAPNPRTARFCADCGQRLSP"
BASE COUNT         1280 a         2735 c         2790 g         1369 t
ORIGIN      
        1 cttcaccaat cggcgggatg ccgggcgtca agaaattgca ctcgttcagg ttttggggcg
       61 aggggaccag tttgcaggct tgggttcggt tgcgaaagcg cgcacacgcg gtggaagctt
      121 cggcggcgct cgctcgagcg cactcggtaa tttgatggat cgccgcgacg caagcgtccg
      181 tgaccgggcc gccgggagcg ggcacgctga ggccatgcag gcattggtct cggtagaagc
      241 gcttgcaagc cgaagcgtcg tcgatggcgc cgcacgcctc ggcggcttca cagcgtgctt
      301 cctcgatggc tcggcagtct tcgattccgg tcgcgtccgt gccgcagctc gccgcgagaa
      361 cgagcgcccc gaggaacggg gccaagagag aagcggcggg agaggcgcgc atatggcccg
      421 aggccctagc acggccgcgg caaatcggaa aaacttcgcg gcttgccctt ccgtcggcag
      481 gcgctcacac tcgcccgccg atggcgcgtg ccatggttct ctgcgctggt ttcggtaccc
      541 gcttgcgtcc attgacggac gagctgccga agccgctgct gccttatgga gacaggagcc
      601 tgctcgagca cgccgtccgg tcgttccagg cggcaggctt tgcggaggtc gtggccaacg
      661 tgcaccacct ggccgagcgc ttcgaggcgg cgctcactgg ctttgggggc tcggtcgcgc
      721 tcgtgcacga acccgtgctg cgcggaaccg ccgggggcat tgcgggagct cgccgcgcgt
      781 tgggcccgtc gccggttctc tgcacgattg gcgacgtcgt gctcgagcgg atcccgaccg
      841 agctcgtcga cgcggctgga ttcggcgggc tcgtgctcgc ggtcgctccc cgtgttgccg
      901 gcagtggcac ggtcgggatc ggcgcgcatg gcgaagtggt gcggctccgc ggcgagcgct
      961 tcggtcaaga ggtgagcggc ggcgactaca tcggtttgtg tgcgctcggc gacagggcgc
     1021 tcaccgagct acctgagatg gggtgtttgg tcggtgacta tgcgttgccc gtgctgcgcg
     1081 agggcggcga ggtccgaacc tttgcttata gaggtgctta tgtcctcccg ggcgacgatc
     1141 tgggctcgta cttcgcgagt cagctcgcct ggctcgccga gcggggtttg gcttcgttcg
     1201 tcgccgacga ggcgaaggtg agccgaggcg tgctcgtgga cgaaacgttg atcggcgcgc
     1261 gagcgaccgt gacgggaacc ggagttctgc gccgcgtcgt ggctctaccc ggcgcgacgc
     1321 tcgaggcgcc cctcgaatcg gcgatagttg caccgagcgg gcggataatc cgaattgatt
     1381 cgtaggttca tgcgtcgttg ctctcgtcga atctcgaggt gtcggtcgtg ctgaaggtca
     1441 cgccgctcga ccttagaccc gacgccgcgc aaatcgcgcg gaggctttgg gatcgcgagg
     1501 gactcgtgct cgtgcgcgtc ggtgcgttca ctttcgtcgc gtgcgatccg gtcgaggtgg
     1561 cggagcgctt cgatcccgag ccgacgctcg cgcggcgagc gctgctgcat ggagaaagtc
     1621 cacgctggtt cgggctttta ccttacgaaa gcttgcgtgg tggtgagggt gagctcgaaa
     1681 gcgatactcg cgccgcggct ctcgtcgagc gcccgctttg gttgcgctat ggggcgctcg
     1741 ttcgcgtggg tgacggtgtc gagctgatcg ccgactccga ggacgcggcg cggcagctcg
     1801 cggcgctgct cgagcgtgct cctcgggctc gggacgtcga gctgagcttc gatggcgcgc
     1861 tcgaagctcc aggcttgcac gcattgcggg ttcagaaagc gctcgaagaa atcgctagcg
     1921 gcaacatcta cgaagtcaat ctcgcgcgtc gcttcgagct ctccgtgaac ggctccgcgc
     1981 cggagctctt ggccgcgttc gagcgctcgg gaaagatgcc gcactccttt gcgctcgagc
     2041 atcgagatct caaagtcgtc gccgtgtcac ccgagctctg tctgcgcctc gagcctgacg
     2101 gcgagctcat gacgagtcca atcaagggga cgcgcccgcg ccaccccgat ccgctcgaag
     2161 accaaaggct cgcgcgcgag ctcgaggagg atcccaagga acgcgcggag ctcacgatga
     2221 tcatcgacgt cgagcgcaac gatttcgggc gtgtcgcgac gctggggagc gtgcgcttga
     2281 gtgagccgcc gcacgtcgtg tcgctgcccg gtgtgcacca ccgcgtcgcg agcgtgacgg
     2341 ctcaagtggc gcctgagctc ggccgtgatg agctattcga ggccgtgttg ccgagcggca
     2401 gcgtcaccgg agcccccaag cgacgtgcaa tgcagctcat ccgagagctc gagccccacc
     2461 ggcgcggcct gtacacggga gccgtcgggt tcgtgcgtca cgacgggggc gtcgagctca
     2521 aaatggcgat ccgtacgctc gtagcgcgcg gctcgagggg gcactacttc gcgggcggag
     2581 gcatcgtggc cgacagtgtc cccgagcgcg aagtcgaaga aaccctgtgg aaagcggagc
     2641 gtcttctctc gctcgcggca ggctgagtcg gccgcggggc ccgaaacgtg cgccaggaat
     2701 tgggccgctt ctggccgctc gcggtacccg tagttgcagg gcgcattgac gcccggtcga
     2761 ttcgccatgc agcccgttaa cgttcgtaac ctcgagcaga tccaagaaca cgacggcgtc
     2821 cccaaatcgt cgcgtctcgg ggcgctgctg ctcgcggcgc tcgcctccgc ggcggtcgtc
     2881 acatcgagcg tcatgatgag tcggcagaaa gggccgccca cggcgtcgag cgcggaccct
     2941 cttgccgagc tcgtcgccaa cgcgaaggcg caggcgcctg cggagcgcct cgaaggcaaa
     3001 gaagtcacgt tcccggccgt gttgagcgat cgcgaaaagc cgaccacggc gctcgccgcg
     3061 gtcaaagacg agcgcggccg cctcgttgcg cgcgacgaac ccgaggcgcc cgcggcgccg
     3121 acgagcccac cgccggcggc agatcgcttg ccggtggtcc cattgccgat cggtacgttg
     3181 ctcaccgcga cgccggtgac caccgagccc aaggaccgcc tcacgtcgct cgcatcggat
     3241 gccgcccgcg gcgccgagag cagctcgctc gctccagctg gtagcgacgg cggcttccag
     3301 cttcaggtcg caagcttcaa agatcagaac gacgcggaca agctcgtcga cgagctccgc
     3361 caccgcggac accgcgcctt tcgccaggcg gcgtacgtcc ccgaacgcgg gctttggcat
     3421 cgcgttcgta tcggcccatt caagtccaag ctcgaggcgc agaagtacaa ggccgagttc
     3481 gagcgcaccg agcgcgtctc gcccttcttg gtcgaccccg aaaaggtgaa gcaggcggag
     3541 cagaaccgcg cgatgcggct cgccgcgcaa gagaagaagg gcggccgcca catcgcggtc
     3601 cccgcgagcg agtgacgctc acttgccttg ttttcgcatc agctgctgac gggccgcgag
     3661 cgaaatagcg ccgggaacgt tcttgctctt ttgtagcgcg cgcaggtcgc cgtcgcgcag
     3721 gtggggcatg aacctcgccg cgtaggtgag gggcgtgcgc ggattgctca ccaggttgag
     3781 cttcacctgg tagttgcgcg taaagtcacg gttcagcgcg atcgtgcgca agacctcctc
     3841 cgacacggcg cggctcgcgc tgatctgcgc ggcctcgttc tcgcgcatga gcgggctctt
     3901 cacggccgcg gtcgcgacca agcggttcgc gtcccgcacc aacaaaagcc tttcggcggc
     3961 ggtcccgagc gtggcccggc ggatcttttg ggtgaccgtc agctgaccga tgatcgcgta
     4021 gaggggcacg aacttgtcgc gcaggtgctc ttcaccttcc tcgtcgcgct cgtgggtgtc
     4081 ctcgtcgtcg gcgacgtctg ccgtcttcgc gatctgttcg gtgttggtga agagctcgtc
     4141 gtcgtaggtc ggctcttccg agggctcggg gacgagctcg ttcttgatgg cctgcgcggc
     4201 ttctttgaag gcgggcaggt tgagctcgag tccgttgcgc acggcaagct cgatcagccg
     4261 gtccgcagtc gacatgcgaa cgcgcttgtt catgtagagc ttctcgatga cgctcgggta
     4321 ctcgagcatg cgccgctcgt tcgtggcgat gagctccccc gagcgttcgt ccgcgacttc
     4381 ggccatgtgc tcgagcgccg ccgctccgag gcgcggcatg cgtaagagct gctccacgac
     4441 ctcgtgctgg ttcgtgtagg cgtgcgcgag ccggtcgacg acgagcgtcg gaagatcggc
     4501 gctgagcgct ccgttcagga tgggcggggg gagcttgccg agcgtcgcgg tcgcggtgtc
     4561 acgcagcttc tcgtcctcgc tcgcgctcag ggcggcaacg acgatcacga tctccgcggg
     4621 cttcagcccc ggcagcacgc cgcgcgccgc catgagcttc atggggaggg gggtatcgac
     4681 gtggagcgcc ttttgcgcag gaccgggaag ggaaccgagc tcggtgtcgg acagcatgtg
     4741 ggtcgcgacg cgacgccggc acccaaatac tagccttcgt cgcggggccg gtaagcatac
     4801 acctttcggt gcgcccgccc gaggagcttc ttcattcgag cccgccgtgg tagcggacgg
     4861 ccttctcccg gcgtcctccc ggataccgct cgaggtactc gagcgcgcgt tgccgggcta
     4921 ccacgctctc cccggcgcgc gaccagcttt cgacctcgcg agcgagcgca tcctgagcga
     4981 gcgcaccgtt cggcgagagt cgccgagcct tgccgaaggc gtctgcggct tcgcgtggcc
     5041 ggcccaaact gtcgagcaac gtgcgcccca aagtgaacgc ggcgagcggt gccctcgagt
     5101 catggggatg accccgcagc actcgctcga gcaacggcaa cgcgagcgct gcgtgcccgc
     5161 tcaagcgcgc cacgtcggcg gcgaagagca gatcgcccgg ctcatcacgc accgcgttgc
     5221 tgccttcggc ttgcatgcgc tcgaaagcgc tccggtagtc cccatcctgc gcgagtgtgc
     5281 gccacgaaga aggcgcggcc gccggcgctc gccgcgtcga aggcgcattc gaattcggcg
     5341 cctcgctgct cggttccacc ggcggcaccg gctcgatggc gggaacttcg gcgctcgccg
     5401 ccgagcgagg cgcactctgc ccggtacgaa acgcgccttc ggacgcaaag atgcgctcgc
     5461 cggcaccgag gatgcgatgc tgcccatcga accccacgtc cactcgcccg cgctccactt
     5521 cgactcggac gccgccgctc tcgaccgcga cgctgaacgc ggtgccgagc accgtgacga
     5581 gcacgtcgcg cgcctgaacc tggaagtgac gaccctccac gtgcgccacg tcgaagtgag
     5641 cgccgccggc ttcgagccgc acccgcaccg cctctcgcga cacctcgacc ggctcgacgc
     5701 gcgcgtcggt gctgagagcg gtgacgcgcg tgccgtcggt gagctcgaaa agattacgca
     5761 catcgtggcg caatggcgct tgcgccacga tgtgcgtgtt gccgaacacc tgacgatacc
     5821 ccgtatgccc caagaacaac gccgccaccg ccaacgcgac gcccccgagc gctcgaatcg
     5881 tcgtgcgccg ccgatcgagc ccccgctcga ccctgaagcg aagctcccgc tcgcgcccgg
     5941 gggtccactt gggactcacc cgctcgcgag cgtttgctac gcgctcgagg aactcagcca
     6001 tccgaaaatg cctcctccaa ggtgcgagcc gcagccgaga tgcggcgctt cgccgtcgcg
     6061 agagaacagc ccgaaagcgc cgcgacggcg tcgagctgct caccctcgat gtgacggagc
     6121 gtccaggcaa tccgctggtc ggcgggcaac gagtcgagaa cgcggtaaac gcgctggagc
     6181 agcgcgcgct gttcgggact cgcctggtcg tctgcgatga cgtcgtatgc ggctgggtcg
     6241 tcgactccga agaaggagcg gaccctccgc atttgaagct tgcgacgcga cacgcgcacg
     6301 gcgatgcgcg cgagccatga tttcaccgcg agcggatcgc gcacgcgact gagtccgcgc
     6361 acggccacca tgaagacctc ctgcacggtg tcgtccacgt cggcatcgcg tccgagcagg
     6421 cgatgcgcga ccgcggcgac gtacggagag tagcgcttga acacgctctc gaagtcgagc
     6481 gcggcttcgg gcgccgctac cacgtcgaac tcgccggttt cgcgcggggc gaccggcggg
     6541 acgatctcga gggcgacgcg agcgcgctgc attgctgccc ctgagtgtgg cccgggggaa
     6601 gaaacggctc acgcgaattc gatttgagcc gtgcccgcgg tttgccggca ccgagcgagc
     6661 atgccgaccc tgccgctccc tccgccgtaa agcgcccgag cgccggacgc ccggtgcgaa
     6721 atatcgtcca aactacgcga aaacgtccgc tctggagccg gatcccagcg ctggttgcga
     6781 gcccatcggc tcgaagctac gttgaacgcg ccgggttcgt cgctcgtttc gagctcgaac
     6841 cgaaaatcaa ggagctgcgg atgggcttga tggacttcgt gaagggtggc gtcggacgga
     6901 tgatgatcgc gcgtccggac gagcacaagg acaaggtcgt ctacaagcac ccggatcaga
     6961 ccttcccatt ctggagccag ctcaccgtcg actcggacga ggtttgcctc ttcttcaagg
     7021 atggaaccta cgtaggtgcg ctgcctccgg ggcggcacac cctgcagacg cagaacatcc
     7081 cgttcttgaa tgcgctcgtc gacaagttca ccggcggcaa cgtcttcatc agcgagatct
     7141 acttcgtcac gacgcggccc ctctacaatc aaggctttgg cgacacgatc gggtcgatgc
     7201 gcgatccgga gctcgacatt cgcgtgacgc cgcgggcttt cgggacctac tcgttccgcg
     7261 tcgtcgatcc gctcaagttc gtggtgggct ttcgcgggca aacggggggt gaccccgagc
     7321 tgtcgctgcg ctgggtgcgc gatcagctct tcatgggcct caaggcgacg ctttcgcggc
     7381 tcatcaagtc gggggagctc acgctgctcg atctgggcac gtgcggtccc gacatagcgc
     7441 gcgcgatcgt ccaaaactgc ccggacttgg cgaacatcgg cgtgcaggtg ctcgagatcg
     7501 cgaagctcaa catcaacctc agcgacgagg accagcaccg catcgacgcg gcccagggag
     7561 aaatcgtcca ggcgaagctc gcggcgcgca aagcgcggat tggcatcgcg caagccgagg
     7621 ccgaggcgca ggcccgccag tatcagctcg atcaggagtt tttgaatcgc gcccgctacg
     7681 tgaatcagct cgacatgggc cgctaccagc aatacgccgc agcggaggcg agcctcggcc
     7741 tcggtgcggg catggcgcag ggaggcgagg gtgcgggcgc ggctgctgcg gcggcggggg
     7801 tcgctcaggg tctcgctctg ggcgccgggc tcgccgcgcg accgccgtat ccgcccccgg
     7861 gctatccagc ggccggttat ccgcccccgg gctatccacc tcctggctat ccagtgccgc
     7921 ccgccggtta ccccggttac cctccgcccg gaccgcctcc tgcgcctgcg gtagccgctg
     7981 ctccggccgc ttacgctcct ccggtgcctc cccccccggt agcccccgcc gcggcatcgg
     8041 gctccgacgc tcaggttcgc tgcgcgagct gcaacgcccc gaacccccga acggcgcgct
     8101 tttgcgcaga ttgtgggcag cgcctatcgc cgtgagcgga ggggaaatgt cccgcgtgct
     8161 cgtcagcggg ctcc
//
LOCUS       DASTKS010000700         8222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1003748, whole genome shotgun sequence.
ACCESSION   DASTKS010000700 DASTKS010000000
VERSION     DASTKS010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003748"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFQ35_14435"
     CDS             complement(<1..964)
                     /locus_tag="VFQ35_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET9931895.1"
                     /translation="MKFGFSTQVLRARRALLGLALCACSNPSSTNSDQSFSGGAPAAG
                     GTSPGAGGASLAGGSAALPAGASSGGGGTAFSTGGEAGRNGGSTSGGYAASSGGSSAG
                     QASQGGAIVGGTTGVTAGVGSGGAASGGASIGGSTGGSTGGSAGADGNSGSFTLAWQD
                     DFDALDSSRWQAQNFTWEGNAAQFTPDNVSVANGALSMSLTTAPSGSTKPYRGVELRS
                     TKTLTYGKVSARMRFARGSGVVSGLVLFYTPYPNCDWNEIDIEHLGNSSRTSQLNAMV
                     YTGAPNDACTASVSPTQDPLVVSLGVDAEADYHVYDIEWTPLGVR"
     gene            complement(1019..1840)
                     /locus_tag="VFQ35_14440"
     CDS             complement(1019..1840)
                     /locus_tag="VFQ35_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9931896.1"
                     /translation="MIAVPLTRLDLILRLAKTAVLVVGVLLAGCSDQSSKEGSFSLLY
                     RDDFDALDFTRWQVMTHSWDTNLALFSGESVAVRDGSLELSLLKAPDGTMDSGGTAKP
                     FFGAEVRSRDTLTYGRVRTRARFARGPAVISSLVTIYTPWPADNWNELDIESLGAKPE
                     SLQFNAQVYLGTLPAPTKPVSPSPDEAVQALGFDASADYHVYQIEWTPREARFSIDDQ
                     VARTWTRHIDLMNLPQNVLLTIWASSVASWAGAVNDDTVKAKASYDWVELYEYAR"
     gene            1937..2743
                     /locus_tag="VFQ35_14445"
     CDS             1937..2743
                     /locus_tag="VFQ35_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9931897.1"
                     /translation="MSPSAPLFQPFPMLEGRSAQAWRHQPAFRRPRHFHEEPECNLAL
                     RGRAVVSVGAKTFTLSPGSLGVFWPGQDHELLEASADLELVVMALRPELASKIDGWQH
                     SRSSNSIELDARRVARIGERASAVNAMSDRLAVESTLASVFDEIASAFGRSHSLSRRA
                     ALALQQSSACSGDELSRRLRAHRSNVSRTFKDDWGVTLVDFRARVRLMKFIQLVDSGQ
                     SLTEAALAADFGSYAQCHRVFQRFLRCAPRTYFAGARSTIDSALAEPAGR"
     gene            3036..4892
                     /locus_tag="VFQ35_14450"
     CDS             3036..4892
                     /locus_tag="VFQ35_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9931898.1"
                     /translation="MITGGNVRPEQLQAAMDLAPLAMVLVDADGQIRFVNQACERMLG
                     FDEEELLGKPIDVLWAEPSRGEPANEARQVLLDPKRHEASLRELVACRRDRVELPVEV
                     SFTPAATSAGDLTLVSLRDATSQADADAMFRALVDAAPSAMIVVRHDGRIAFVNQRTM
                     ALFGYGRDDLIGSPIELLIPERYRREHPALVSGYMSRPVARPLGAGRELWGRRRDGSE
                     VPVEIGLTPIPTSRGELILASVIDVSERHRAEAVRERLAAIVEGSEDAILSKTLDGTI
                     TTWNGAAERLFGYSAAEAIGQSIALIVPESMRDEEAGLMERIRQRERVQQFETKRRRR
                     DGILVDVSITLSPLGNGSGEIVGASSIVRDVSALKLSNAELARSNAELARSNAELEQF
                     AYVASHDLQEPLRMVANYTELLRKNYGTRLDERADKYIHYASDGARRMQTLVNDLLAY
                     SRVGSQGKPMVSVAAGAVLGRAIASLGASINESGAVIEVGELPSVCADEVQLQQLFQN
                     LLSNAIKFRAADPPRIVISAKPAGEKVEFSVADNGIGIDMRFVGRIFQMFQRLHERDK
                     YAGSGIGLALAKRIVERHGGNIWVESALGVGSTFYFTLASASAGKGGLPCSH"
     gene            4880..5302
                     /locus_tag="VFQ35_14455"
     CDS             4880..5302
                     /locus_tag="VFQ35_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931899.1"
                     /translation="MQPLRVLLVEDNPADAELTRDTLESGKVVIDIEVVVDGAQALAY
                     LRREPPYADAPLPDLVLLDLNLPKLSGREVLAEMRKQPALRALPVVVLTSSDAEQDIL
                     RSYELGANCYVTKPVGLEAFQSIVRAVESFWFTVVKLP"
     gene            5299..6750
                     /locus_tag="VFQ35_14460"
     CDS             5299..6750
                     /locus_tag="VFQ35_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931900.1"
                     /translation="MSPYLADVCRVLVIEDNPGDAEMIRESLLEGQYTVSAASQLSEG
                     LRLLESDVPDVVLLDLNLPDGTGVECVDRVRTHANNVPIVVLTGGSDDALALACIRAG
                     AQDYLKKGEVRPADAQRAIAYAMARAKEVTERGRADALERQLAAIVDNSREAILSTTI
                     DGVVVSWNRGAERIFGFSPLEAIGRSASEVIRRVDGGSAVPPLEPESGDGQPIEIQCL
                     RKDGMRVSVSFVWSRFLDASGRGVALSAIGRDVTEARRRDDELKQRNAELEARDHQMR
                     ALTVRLHEIREGERTRISRTVHDELGALLTALKFDLRWCEKRVSVDRAVAERLREAGG
                     LVDQTVKTVQRIAVELRPSALDTLGLPAAIRDEVRRFSERTEITPQLRLTAETPTKPE
                     VATVLFRVFQELLTNVARHAKASHVEVALESIDGHWVLRVTDDGVGVGHDNPGARSLG
                     LLGIRERLQALGGSFLLEGEAGLGTRAVARVPN"
     gene            6754..7401
                     /locus_tag="VFQ35_14465"
     CDS             6754..7401
                     /locus_tag="VFQ35_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9931901.1"
                     /translation="MNPNSIQNVLVADDHEVTRRGVKDILQETFSGVQVAEAANADAV
                     LGLLGERPWNLLLLDVMMPGSNVLDLLKQIRRIDPKVPILMLTAATEVEYVLQTMQAG
                     ANGLIHKHRATEELIEAIHRVADGGTYLHPETAGAVAASLRPAPSRPVHTKLSERELE
                     IFRLIALGRAIKEIAAELGLSDKTIATYVARIREKTGLTSYVEIARYALQHRLVD"
     gene            complement(7439..>8222)
                     /locus_tag="VFQ35_14470"
     CDS             complement(7439..>8222)
                     /locus_tag="VFQ35_14470"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET9931902.1"
                     /translation="KQRSSGIRVSSGVPELDKMCDGGMYRDSIILVSGATGTGKTLMV
                     TQYIKAAIEAGERAILFGAEESREQLTRNAASWGVDFEKAEADGLLRIVCRYPEVMGL
                     EDHLLHMKRDINAFRPKRVAVDSMSAFERVSTPKSFREFVIALTSAVKQLEITGLFTN
                     TTSLLLGGESITETHISTITDTIILLRYVELHGEMRRGLTVLKMRGTFHDKGIREYVI
                     DNQGLHVKAPFRGVHGILNGTPTYTVDDERSRLGDMFRSERE"
BASE COUNT         1563 a         2631 c         2590 g         1438 t
ORIGIN      
        1 agcgcacgcc gagcggcgtc cactcgatat cgtaaacgtg atagtcggct tcggcgtcga
       61 cgcccaagct cacgacgagc gggtcttggg tcggcgaaac ggaggccgta cacgcgtcgt
      121 tcggtgcgcc agtgtaaacc atcgcgttca gctgcgacgt gcggctcgag tttccgaggt
      181 gctcgatgtc gatttcgttc cagtcgcagt ttgggtaagg cgtgtagaag aggacgagcc
      241 cagagacgac ccccgaaccg cgagcgaagc gcattcgcgc agacacctta ccgtaggtca
      301 gcgtcttggt ggatcgcagc tccacgcccc ggtatggctt ggtcgagcct gacggcgccg
      361 tcgtcaacga catcgagaga gccccattcg caacgctcac gttgtcgggc gtgaactgag
      421 ccgcgttgcc ttcccacgtg aagttttgag cttgccagcg actcgaatcg agcgcgtcga
      481 agtcgtcctg ccacgcgagc gtgaaactcc cagagttgcc gtcagccccg gcagagccac
      541 ccgtcgaacc acccgtggaa cctcctatcg aagcaccgcc cgacgctgcg ccgccggacc
      601 cgacgcccgc ggttacgccc gtcgtgccac ccacgattgc accgccctgg ctcgcttgcc
      661 ccgcggagct tccgccgctc gaagccgcgt agccgccgct cgtgctaccg ccatttcgcc
      721 cggcttctcc gccagtagaa aaggccgttc ctcccccgcc actcgaagcc ccagccggca
      781 atgctgcact gccgcccgcg agcgaagcgc cgccggctcc gggcgaagtg ccacctgccg
      841 caggcgcacc accgctgaag gactgatcgc tgttcgtcga actcgggttc gaacaagcgc
      901 acagagctag acccagcagc gctcgacgag cgcggagaac ctgtgtcgag aatccgaact
      961 tcatgcctga cgaaacctca gacggggaac cgacgggcac tcgaggccac cctatctttc
     1021 aacgcgcata ttcgtagagc tcgacccagt cgtacgaggc cttggccttc acggtgtcgt
     1081 cgttgacggc accggcccag gacgcgacag atgatgccca gatcgtaagc aatacgttct
     1141 gcggcaaatt catcagatcg atgtgacgcg tccacgtccg cgcgacctga tcatcgatcg
     1201 agaagcgcgc ctcgcgcggc gtccattcaa tctggtaaac gtggtagtcc gcgctcgcgt
     1261 cgaagccgag agcctgcacc gcctcgtcgg gcgaagggct caccggcttc gtcggcgccg
     1321 gcagcgtgcc gagatacacc tgggcgttga actggaggct ctccggcttc gcccccaggg
     1381 actcgatgtc gagctcgttc caattgtcgg ctggccacgg cgtatagatg gtcaccagcg
     1441 acgagatcac cgcggggcca cgcgcgaatc gcgctcgcgt cctcacccgc ccgtaagtaa
     1501 gagtgtcgcg cgatcgcact tccgccccga agaacggctt ggcggtgcct cccgagtcca
     1561 tcgtgccgtc gggcgctttc aagaggctca gttcgagcga cccgtctcga accgcgacgc
     1621 tctctccgga aaatagtgcg aggttcgtgt cccacgagtg ggtcatcacc tgccagcgcg
     1681 tgaagtcgag agcatcgaaa tcgtcgcgat acaacaaaga aaaggaccct tccttcgagc
     1741 tttggtcgct acagccggcc aggagtacac caacgaccaa cacggccgtc ttcgcgagcc
     1801 gaagaatcag gtccaaccga gtgaggggca ccgcaatcac gcgagagcta tgccgcggcg
     1861 cccgagctcg aacatctcac tttgtgccat tttgcgcgga ggcgacgggc cgcgaagaga
     1921 cgctatactc gagcttgtga gtccgagcgc acccctcttc cagccgttcc ccatgctcga
     1981 ggggcgctcc gcacaggcct ggcggcacca acccgcgttc cgtcgaccgc gccacttcca
     2041 cgaagagccg gagtgcaacc tggcgcttcg gggccgcgcc gtggtttcgg tcggcgcgaa
     2101 aaccttcacg ctatcgcccg gtagcctggg ggtcttttgg ccggggcaag accacgagct
     2161 cctcgaagcg agcgcagatc tcgagctcgt ggtcatggcg ctccgacccg agctcgcatc
     2221 caagatcgat ggctggcaac actcacgttc cagcaattcg atcgagctcg acgcacggcg
     2281 cgttgcccgc atcggggagc gagcttcggc cgtcaacgcg atgtcggata gactcgcggt
     2341 ggagagcacg ctcgcgagcg tcttcgacga gatcgcgagc gccttcggca gatcgcattc
     2401 cttgagccga cgagccgcgc tcgcgcttca gcagagctcc gcctgctcgg gagatgagct
     2461 atcgcgtcgc ctccgcgccc accgaagcaa cgtgagtcgg accttcaaag acgactgggg
     2521 cgtgactctc gtggacttcc gcgcgcgcgt gcgcttgatg aagttcattc agttggtcga
     2581 ttccggacag agcctgaccg aagccgcatt ggcagccgac ttcggcagct acgcccagtg
     2641 tcatcgcgtg tttcagcgct tccttcgctg cgcgccgcgc acttatttcg cgggcgcacg
     2701 gtcgaccatc gattcggccc tcgcagagcc tgccgggcgc tgagagccag tcctaaatcc
     2761 tggaccgagc ccggcaaaga gacgcgagca gtccaaggcg agacgacgag cgtactgaag
     2821 tacgccgagg aggagcaacg aaggaatgcg aagcggatct gccgccgggc gacgggaggg
     2881 atttaggatt ggctctacga gtgccgcgag tttttctgcc ccgggcgcga agcggcgcga
     2941 ctggcgagtg ccgctctttg ccgtcgctgt agccccatgg taatgagcgg cccagtgctc
     3001 taatctctcg gcagacctct gtttcaggca gatacatgat aacgggggga aacgttagac
     3061 ccgagcaact gcaagcagcg atggacctcg caccacttgc aatggtgctg gtcgacgcag
     3121 acggacagat ccgcttcgtg aatcaggcgt gcgaacgcat gctgggtttc gacgaggaag
     3181 agctcctggg aaagccaatc gacgtactgt gggccgagcc gagtcgaggg gaaccggcga
     3241 acgaagcccg tcaagttttg ctcgacccaa agcggcatga agccagcctt cgcgaactag
     3301 tcgcttgccg acgcgaccga gtcgagcttc cggtggaggt ctcgttcacg ccggccgcga
     3361 cgtcggcggg agatctcacg ttggtttctc tccgtgacgc gacttcgcag gcggacgccg
     3421 atgccatgtt tcgcgcattg gtcgacgcgg ccccgagcgc aatgatcgtc gttcgtcacg
     3481 acgggcgcat cgccttcgtg aaccagagga cgatggccct cttcgggtac gggcgggacg
     3541 acctcatcgg ctcgcccatc gaactcttga tacccgaacg ttaccgacgt gagcaccccg
     3601 cgctggtgag tggatacatg tcgagacccg tcgcgcggcc attgggcgcc ggccgtgaac
     3661 tctggggcag gcgccgtgac ggcagtgaag ttcccgtaga aatcgggctc acgccgattc
     3721 cgacttcccg cggcgagttg atcctcgctt cggtcatcga tgtcagcgag cggcatcgtg
     3781 cggaggccgt tcgcgagcgt ctggccgcca tcgtcgaagg gtccgaggac gcgatcctga
     3841 gcaagacact agacgggacc atcacgacat ggaacggagc cgcggagcgc ctcttcgggt
     3901 actccgccgc ggaggccatc ggtcagtcga tagcgctgat agtgccagaa tcgatgcgcg
     3961 acgaggaggc ggggctgatg gaacgtattc gtcagcgcga gcgcgtccaa cagttcgaga
     4021 ccaagcgccg gcgccgcgac ggtatcctgg tcgacgtctc catcacgctc tcaccgctcg
     4081 gcaacgggtc cggcgagatc gtcggcgcat cgtcgatcgt gcgcgacgtg agcgcgttga
     4141 agctcagcaa cgccgagctc gcacgcagta acgccgagct cgcgcggagc aatgccgaac
     4201 tcgagcagtt cgcctacgta gcctcgcacg atctccagga gccgctgcgg atggtggcga
     4261 actataccga gctcttacga aagaattacg gcacgcgcct cgacgagcgc gctgacaagt
     4321 acatccatta cgcgagcgat ggcgcacgcc gcatgcagac cctagtaaac gatttgctcg
     4381 cctattcgcg ggtaggctcg cagggtaaac cgatggtctc ggtcgccgcg ggagcagtgc
     4441 tcggcagagc cattgcttcg ctaggcgcct cgatcaacga gagtggtgcg gtaatcgagg
     4501 tcggagagct acctagcgtg tgtgccgacg aagttcagct ccagcaactt tttcagaatc
     4561 tactgagcaa cgcgatcaaa ttcagagccg ccgacccgcc gcgaatcgtc atttctgcca
     4621 agcccgctgg ggaaaaggta gaattttcgg tcgccgacaa cggcatcggc atcgacatgc
     4681 ggttcgtcgg aaggatcttc cagatgttcc agcggcttca cgagcgcgac aagtacgcgg
     4741 gaagcggcat cggcctggct ctcgcgaagc gcatcgtcga acgccacggc ggaaacattt
     4801 gggtcgagtc agcgctcggc gttgggagca cattttactt tactttggcg agcgcctcgg
     4861 ctggaaaagg aggcttgccg tgcagccatt gagagtactc ctcgtcgagg acaaccccgc
     4921 cgacgccgag ctcacccgcg acactctcga gagcgggaaa gtcgtcatcg acatcgaggt
     4981 cgtggtcgat ggtgcgcaag cattggccta tctgcgccgg gagcctccct acgcggacgc
     5041 gccacttcca gacctagtgc tgctcgactt gaaccttccc aagctcagtg gacgcgaagt
     5101 cctggctgaa atgcgtaagc agccggccct gcgagcgctc cccgtggtcg ttctcacctc
     5161 ttccgatgcc gagcaggaca tcctgaggag ctacgagctc ggagccaatt gttacgtgac
     5221 caagccggtg gggctagagg ctttccagtc catcgtccga gcagtagaga gcttttggtt
     5281 tacagtggtg aagctgccgt gagcccctat ctggcggacg tatgtcgggt gctcgtcatc
     5341 gaggacaacc ccggtgacgc ggagatgatc cgggagagcc tgctcgaagg tcagtacacc
     5401 gtctccgccg cgtcccaact cagcgaaggg ctacgcttgc tagagagcga cgtgcccgac
     5461 gtggtcttgt tggacctcaa cctgccggac ggcaccgggg tcgagtgcgt cgaccgagtg
     5521 cgcacgcacg caaacaacgt tccgatcgtc gtgctcacgg gtgggagcga cgacgcgctc
     5581 gccttggcct gcattcgcgc gggcgcgcaa gactatctga agaagggcga agtaaggccg
     5641 gcggacgcgc agcgagccat tgcatacgcg atggctcgcg cgaaggaggt caccgagcgc
     5701 ggacgcgccg atgccctcga gcgtcaactc gccgcaatcg tcgacaactc gcgcgaagcc
     5761 atcctcagca cgaccatcga cggcgtcgtc gtcagctgga atcgtggggc cgagcgcatc
     5821 ttcgggtttt cgcccctcga ggccatcggg cgttctgcaa gcgaggtaat tcgccgcgtc
     5881 gacggaggta gcgcagttcc accgctcgag ccggagtccg gagacggcca gcccatcgag
     5941 atccaatgct tgcggaagga cggtatgcgc gtctccgtat cgttcgtgtg gtctcgcttc
     6001 ctggacgctt ctgggcgcgg tgtggcgcta tccgctatcg gccgtgacgt gaccgaggcc
     6061 cgccgacgcg acgacgagct gaagcaaagg aatgccgagc tcgaggcccg agatcatcaa
     6121 atgagggcgc tcacggtgcg cctccacgaa atccgcgagg gagagcgcac gcgcatctcc
     6181 cgcaccgtgc acgacgagct cggggcgttg ctcacggcgc tgaagttcga cttgcgctgg
     6241 tgcgagaagc gcgtctcggt cgaccgcgcc gtcgcggaac gactgcgcga agcgggcggg
     6301 ctggtcgatc aaaccgtgaa gacggttcag cgcatcgcgg tcgagctgcg tccgagcgcg
     6361 ctggacactc tgggcctccc tgccgccatt cgcgacgaag tccggcgctt cagcgaacgg
     6421 accgaaatta cgccccagct tcggcttacc gccgagacac cgacgaaacc ggaggttgcg
     6481 acggtgttgt ttcgcgtgtt tcaagagctc ttgacgaacg tggccagaca cgcgaaggcg
     6541 agtcacgtcg aggtcgcgtt ggaatccatc gacgggcact gggtgttgcg agtgaccgac
     6601 gacggcgtgg gcgtcggcca cgacaacccc ggggcccgct ccttgggctt gctcggaatc
     6661 agagagcgct tgcaggcgct ggggggcagc ttcctcctag aaggagaggc gggcctcggc
     6721 acacgagccg tggcgcgcgt acccaactga aagatgaacc cgaacagcat acagaacgta
     6781 ctcgtcgccg atgatcacga agtcacacgg cgcggggtca aggacatcct ccaagagacc
     6841 ttctcgggag tccaggtcgc cgaggcggcg aacgcagatg ctgtcctcgg gttgctcggt
     6901 gagcgtccat ggaacctgct gctcttggac gtcatgatgc cgggtagcaa cgtcctcgac
     6961 ctcttgaagc agatccgccg aatcgacccc aaggttccga ttctgatgct gacggcggcg
     7021 accgaagtcg agtacgtgct ccagacgatg caagccggcg ccaacggcct cattcacaag
     7081 catcgcgcca ccgaagagct gatcgaggcg atccatcgag tcgccgacgg cgggacctat
     7141 ttgcacccgg agacggcagg agccgtggcc gcgagcttac gccccgcccc gtctcgaccc
     7201 gttcatacga agctctccga acgcgaactc gagatttttc gcttgattgc gctgggtcgc
     7261 gcgatcaaag aaatcgcggc cgagctcggg ctcagcgaca agaccatcgc gacgtacgtc
     7321 gctcgaattc gcgagaaaac cggcctgacc tcctacgtcg agatcgctcg ctacgcgcta
     7381 caacatcgat tggtcgacta agggtgaacg cactcggtcg ttggcgcgcc cgacctcatc
     7441 actctctctc cgacctgaac atgtcgccca agcgcgagcg ctcgtcgtcg acggtatagg
     7501 tcggtgttcc gttgagaatt ccgtgtaccc cacggaaggg ggccttcacg tgcaagccct
     7561 ggttgtcgat cacgtactcg cgaataccct tgtcgtggaa ggtgccccgc atcttgagca
     7621 cggtgagccc gcgccgcatc tcaccgtgga gctcgacgta acgtagcaag atgatggtgt
     7681 cggtgatggt ggagatgtga gtctccgtga tcgactctcc cccgagtagc aacgaggtcg
     7741 tgttggtgaa caagcccgtg atctcgagct gcttgaccgc gctcgtgaga gcgatcacga
     7801 actcacgaaa tgacttcggc gtcgagacgc gctcgaacgc ggacatactg tccactgcga
     7861 cgcgcttcgg gcgaaacgcg ttgatgtcgc gcttcatgtg gagcaagtga tcctcgaggc
     7921 ccatcacctc cgggtagcgg cacacgatcc gaagaagtcc gtcggcttcc gccttctcga
     7981 agtcgacgcc ccaagaggcg gcgtttcgcg tcagctgctc gcggctctcc tcggcgccga
     8041 agaggatcgc acgctcaccg gcctcgatcg cggctttgat gtattgagtg accatcagcg
     8101 ttttgccggt gccggtcgcg ccgctcacga gaatgatcga atcgcgatac atgccgccgt
     8161 cgcacatctt gtcgagctcc ggcacccccg acgacacgcg gatgcccgac gacctctgct
     8221 tg
//
LOCUS       DASTKS010000701         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_558436, whole genome shotgun sequence.
ACCESSION   DASTKS010000701 DASTKS010000000
VERSION     DASTKS010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558436"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFQ35_14475"
     CDS             complement(<1..1063)
                     /locus_tag="VFQ35_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9931903.1"
                     /translation="MSRASPRIAVTGVGMVSALGVGAKPTFDRLCAGDTGIGELTLFD
                     TAGLAAKLAGEVSGLVVPSGSERADALALLAAREALSQAKLQTTGSRLGLVLGSTAAG
                     MLETEAELLASPGNAPETWPRARRLLTHPLASAERSLARALGGIEQSVTVCSACSSGA
                     LALVVAAHWIASGRAERVLAGGVDPLCRMTFAGFGALGVLDAEACRPFDISRRGLTLG
                     EGAGFLLLESEASVARRGVEPLAWFSGWAVGAEAHHVTHPEEGGERAAELIERALAVA
                     GRSRHEVQYVNAHGTGTPANDAMEARAIARAFGDALPNVAVSSSKGQLGHTLGAAGAI
                     EAAICVLALCEGRVPPTRGL"
     gene            complement(1067..1345)
                     /locus_tag="VFQ35_14480"
     CDS             complement(1067..1345)
                     /locus_tag="VFQ35_14480"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9931904.1"
                     /translation="MREDSVADSSLKDEIKHLLVSELDLRHKTSADIDDAAPLFGAGL
                     GLDSLDALQLAMLVEERFGVTVPEGEAGRAVFASVNALADFVERARSG"
     gene            complement(1338..2345)
                     /locus_tag="VFQ35_14485"
     CDS             complement(1338..2345)
                     /locus_tag="VFQ35_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9931905.1"
                     /translation="MNSAAPALKESDLLERCSSACKSLKLQVSRAVVGQDEVVDALLV
                     TLLARGHALLVGVPGLAKTLLVSSIAEALGLSFGRVQFTPDLLPADITGTDVLNDADP
                     GSRPHVSFMPGPIFKNLVLADEINRTPPKTQAALLQAMQERNVTVGGHTHPLPDPFQV
                     FATRNPIEQEGTYLLPEAQLDRFLLEVHVEYPSEDEEREIARRTTSGERPHIEPVVTA
                     DEIRALGQLVPRIPVTDAAVRLAVRLARISRPSSPDAPEVVTKYVRFGAGPRGSQALV
                     LAAKARAALRGEAAADVEDVQAMALPALRHRLVLGYRADVDGVQDADVLRELVQRASR
                     A"
     gene            2472..2651
                     /locus_tag="VFQ35_14490"
     CDS             2472..2651
                     /locus_tag="VFQ35_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931906.1"
                     /translation="MQAGKEATSNQRVLVERPPEGLLRGKAEGPRWLFVVITLAALAL
                     LVAFYARKLRRKFRS"
     gene            2648..>2985
                     /locus_tag="VFQ35_14495"
     CDS             2648..>2985
                     /locus_tag="VFQ35_14495"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9931907.1"
                     /translation="MNRFVQRLLVATLCGVLVYGAFALYTGLEKMRASLDHYAWSSFA
                     VALLLASGNYLVRFMKWQYYLARLGIRGVGWVDSLLVFLSGFVLTITPGKVGEVFKSA
                     VLFRTHGVAV"
BASE COUNT          471 a         1028 c         1007 g          479 t
ORIGIN      
        1 cgagcccgcg ggtcggcggg acgcggcctt cgcagagcgc gagaacgcag attgccgcct
       61 cgatcgcgcc cgcagccccc agcgtatgac cgagctggcc cttgctcgac gaaaccgcga
      121 cgttcggcag agcgtcaccg aaagcgcgcg cgatggcgcg cgcctccatc gcgtcgttcg
      181 cgggagtgcc ggtgccgtgt gcgttgacgt actgcacctc gtgacggctc cgcccggcaa
      241 ccgcgagcgc ccgctcgatg agctcggccg cgcgctctcc gccttcctcg gggtgcgtga
      301 cgtgatgcgc ttccgcgccg acggcccagc cgctgaacca cgcgagcggc tcgacgcctc
      361 ttcgggcgac gctcgcttcg gactcgagca ggagaaagcc agcgccttcc cccaacgtga
      421 gcccgcgccg agaaatatcg aacggtcggc acgcttcggc gtcgaggact ccgagcgcgc
      481 cgaacccggc gaaggtcatt cggcagagcg ggtcgacacc gccggcgagc actcgctcgg
      541 ctcggccgct cgcgatccag tgcgcggcca cgacgagcgc gagcgcgccg ctcgagcaag
      601 cgctgcacac cgtgaccgac tgctcgattc cgccgagcgc gcgcgccaag cttcgttcgg
      661 cgctggcgag cggatgtgtg agcaagcggc gagcgcgagg ccacgtctcg ggtgcgttcc
      721 cgggagaagc caagagctcg gcctctgtct cgagcatccc cgccgcggtg ctcccgagca
      781 cgaggccgag gcgagagccc gtcgtttgaa gcttggcttg cgagagcgcc tcgcgcgccg
      841 ccagcagtgc caacgcgtcg gcgcgttcgc tgccgctcgg gacgaccaat cccgagacct
      901 cgcccgcgag cttggcggcg aggcccgccg tatcgaacag cgtgagctcc ccgatgcccg
      961 tatcacccgc gcagagccgg tcgaaggtcg gcttcgcgcc gacgccgagc gccgagacca
     1021 tcccaacgcc ggtgaccgcg atgcgaggcg acgcgcggga catcggttaa ccgctgcgcg
     1081 ctctctcaac gaagtcggcg agtgcgttca cggacgcaaa cacggcgcgt ccggcttcgc
     1141 cttccggcac cgtcacacca aaacgctcct cgacgagcat cgcgagctgc aacgcgtcga
     1201 gcgaatccaa accaagcccg gcgccgaaga gcggcgcggc gtcgtcgatg tcggcgctgg
     1261 tcttgtggcg caggtcgagc tcgctcacga gcaggtgctt gatctcgtct ttgagggatg
     1321 aatctgccac cgaatcctca cgcacggctc gcgcgctgca ccagttctct cagaacgtcc
     1381 gcgtcctgca ccccgtcgac gtcggcgcga tacccgagca ccagccgatg acgcaaggct
     1441 ggcaaagcca tcgcctgcac gtcctccacg tcggcggccg cttcgccccg cagcgccgcg
     1501 cgcgctttgg ccgcgagcac gagcgcttga ctgccgcgcg gccctgcgcc gaaccgcacg
     1561 tatttagtga ccacctccgg cgcgtccggc gacgaagggc gagaaattcg agcgagccgc
     1621 accgcgagac gcaccgccgc gtcggtcacc gggatgcgcg ggacgagctg ccccagcgcg
     1681 cggatctcgt ctgccgtgac taccggctcg atgtgagggc gctcgcccga agtggtgcgc
     1741 cgggcgatct cgcgctcttc gtcctcggag ggatattcga cgtgcacctc gagcaagaaa
     1801 cgatcgagct gcgcctcggg aaggagatac gtaccttctt gctcgatcgg gttccgcgtc
     1861 gcgaagacct gaaacggatc cggcaagggg tgcgtgtgcc caccgacggt cacgttgcgc
     1921 tcctgcatgg cttggaggag cgccgcttgt gtcttcgggg gtgtccggtt gatctcgtcg
     1981 gcgagcacga gattcttgaa gatgggcccc ggcatgaagc tgacgtgggg gcgcgagccg
     2041 ggatcggcgt cgttcaacac gtcggttccg gtgatgtcgg ccgggagtag gtccggcgtg
     2101 aactgaaccc gcccgaacga gagccccaat gcctcggcga tgctgctgac gagcagtgtc
     2161 ttcgcgagcc cgggcacccc gaccagcagc gcgtgacccc gtgccagcag cgtaacgagc
     2221 aaggcgtcga cgacctcgtc ttggccaacc accgcgcgcg aaacttgaag cttgaggctt
     2281 ttgcaggctg agctgcagcg ctcgaggagg tcggactctt tgagggcggg tgcggcagag
     2341 ttcacggagg cagtgctctt tttgcggcgc gaggccggga accggcagct tagcaccagg
     2401 atgcggcgac ttggggcctc cgcgttcgcc ggttgccgag ccggcccggc gcgtgtaatc
     2461 ccgagcgctc gatgcaagcc ggcaaggaag ccacctccaa tcaacgagtg ctcgtggaac
     2521 gcccacccga gggcctgctc cgcggcaagg ccgaaggacc gcgctggctc ttcgtggtca
     2581 tcacgctcgc agcgctcgcg ctgctcgtag ccttttacgc gcgcaaactg cgccgaaagt
     2641 tccggtcatg aatcgcttcg tgcagcgcct cttggtcgca acgctgtgtg gcgtcttggt
     2701 gtacggcgcc ttcgcccttt acacgggcct cgagaagatg cgcgcgagcc tcgatcacta
     2761 tgcctggtcg agtttcgcgg tcgcacttct gctggcgagc ggcaactacc tcgttcgctt
     2821 catgaagtgg cagtactacc tggcgcgtct cggcatccgt ggagtcggct gggtcgacag
     2881 cctgctcgtg ttcctctctg ggttcgtgct caccatcacc ccgggcaagg tcggtgaggt
     2941 gttcaagagc gcagtgctgt tccgcacgca cggggttgcc gtgga
//
LOCUS       DASTKS010000702         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1003871, whole genome shotgun sequence.
ACCESSION   DASTKS010000702 DASTKS010000000
VERSION     DASTKS010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003871"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFQ35_14500"
     CDS             complement(<1..545)
                     /locus_tag="VFQ35_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931908.1"
                     /translation="MKLGASTALGLGLALFAPPLRAQLPPAGERIQTHDYTIDLSQTP
                     VLAGTRVTGLSGAYVAIAEGIDGVAQNPGAIALRTPWSTDHFDFDVGIGITFSSALAR
                     SDVFNSGRRTLAAPTGEEALTFLNLAATFQIGRWGFGFSSDFQSYSLDRSKSGSTTGS
                     EQLLARFAITHSTIGYGFADN"
     gene            complement(542..1618)
                     /locus_tag="VFQ35_14505"
     CDS             complement(542..1618)
                     /locus_tag="VFQ35_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9931909.1"
                     /translation="MPWVLVLDPPSGDPTLRVAGLALALRLALDAQSAGASAVIVASG
                     SPAATSLSDRRLRIPVLSNAPSDARRVRVPADYVVFRGLFRRIAEHDSLLEAPPPERD
                     LAKEPFPHQSKYGFDAFAVTDDASRRRAESALFRALRKPEDGWTSRWLNRYISLAVSR
                     RLVKTPLTPNQISLGILAIGLGGACAAASGTEFGLPLGAFLLQTQSVLDGCDGEVSRI
                     THRGSKTGEWLDTIGDDLSNYGFFAGASWGLFKLTGSSWYLAVGAVIVLAGFIASGIE
                     YAYLVKIGSGDLLKYPLSQGGQSGALSYIAPLFKRDTFVLLTLLAALAGVLGPVLCVF
                     ALGALGVLASVIATEIRMRRQAGS"
     gene            1835..>2753
                     /gene="thrC"
                     /locus_tag="VFQ35_14510"
     CDS             1835..>2753
                     /gene="thrC"
                     /locus_tag="VFQ35_14510"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9931910.1"
                     /translation="MTRPIYRCPTCGGLLQVVHDLDALRHRSGASWMRLFDERYRQNK
                     WPYGSGVWGKREWVMPQMPDECIVSMYEGGTNLFWADRYGKTLGLTDLWVKLCGNSHS
                     GSFKDLGMTVLVSVVRMAIRDGLKVKAIACASTGDTSASLAAYGAAAGLPVVVLLPRG
                     KISTAQLVQPLAHGALVLGLDTDFDGCMTIVQRLAEEGLVYLANSMNPLRLEGQKTVG
                     MEIVQQFDWQVPDWIILPSGNLGNASALYAGFKMLKELGVVARYPRLVVAQAENANPL
                     YRAWTQGKRVVEPIQAKPTLATAIQIGNPV"
BASE COUNT          496 a          876 c          934 g          447 t
ORIGIN      
        1 tggttgtcgg cgaaaccgta gccgatggtc gagtgcgtga tggcgaagcg cgcgagtagc
       61 tgctcgctgc ccgtcgtgct gccgctcttc gagcgatcga gcgaatacga ctgaaagtcg
      121 ctcgaaaatc cgaaccccca gcggccgatc tggaaggtgg cagcgaggtt caagaaggtg
      181 agcgcctcct ctccggtggg cgcggccagc gtacgccgcc ccgagttgaa gacgtcggag
      241 cgcgcgagcg ccgacgagaa cgtgattccg atgccgacgt cgaaatcgaa gtggtccgta
      301 gaccaaggcg tgcgcagcgc gatcgcgccg gggttctgtg cgacaccgtc gattccttcg
      361 gcgatcgcca cgtaggctcc gctcaagccc gttacgcgcg taccggcgag caccggagtc
      421 tgcgacaaat caatcgtgta gtcgtgcgtc tgaatgcgct cgcctgctgg gggaagctgc
      481 gcccgcaggg gaggagcgaa caacgcgagg ccgaggccga gcgcggtgct cgcgccgagc
      541 ttcatgaccc agcctgccgc cgcatgcgga tctcggtcgc gatgacggac gcgagaacgc
      601 cgagtgcgcc cagcgcgaac acgcaaagca cggggccgag gacgcccgcc agcgccgcga
      661 ggagggtcaa gagcacgaag gtgtcgcgct tgaagagcgg cgcgatgtac gacagggccc
      721 cgctctgtcc gccttgactc agaggatact tgagtagatc gccggaaccg attttgacga
      781 ggtaagcgta ttcgatgccg ctcgcgatga agccagcgag cacgatcacg gcgccgacgg
      841 ccaagtacca gctcgagccg gtgagcttga aaagtcccca agaagcgccg gcgaagaagc
      901 cgtagttcga gaggtcgtcg ccaatcgtgt cgagccactc accggtcttg gagccgcggt
      961 gcgtgatgcg gctcacttcg ccgtcgcagc cgtcgagcac gctctgcgtc tgcaagagaa
     1021 acgcgccgag gggaagcccg aactcggttc cgctcgcggc cgcgcaggca ccgccgaggc
     1081 caatcgccaa gatgcccagc gaaatctggt tcggggtgag cggagtcttc actagtcgtc
     1141 gcgacaccgc gagcgagatg taacgattca accagcgtga cgtccagcca tcctccggct
     1201 tgcgcagggc acggaacagc gcgctctcgg cgcgccggcg gctcgcgtcg tcggtgacgg
     1261 cgaacgcatc gaagccgtac ttgctctggt gcgggaacgg ctctttcgcg agatctcgct
     1321 ccgggggcgg tgcttcgagc aaactgtcat gctccgcgat gcgccggaac agtccgcgga
     1381 agacgacgta gtcggcgggg actcgcaccc ggcgcgcgtc gctcggtgca ttcgagagca
     1441 ccgggatccg caaacgccga tcggagaggc tagttgcggc gggggacccg gacgcgacga
     1501 tcacggcgct cgctcccgct gactgggcat cgagggcgag tcggagtgca agcgcgagac
     1561 ccgcgacccg cagggtcgga tcgcctgatg gaggatcgag gactaagacc cagggcatcg
     1621 gcggcgcgca gcatagctcg ctccgcctct ctgtcctcta cggagcgtac gatcgccgct
     1681 ttcagcccaa agcgcgggtt ttcgggggtg gacaccgggc cggacgacga atggcagggg
     1741 tacggaagcg cggccccagt catcggctat agtcggggcc gtgttgaacg ctgccgaatt
     1801 ccgttgtttc gccggttgcc ccggctcctg gcccgtgacg cgtccgattt atcgctgtcc
     1861 aacctgtggc gggctgctgc aggtggtgca cgacctcgac gcgcttcggc accgcagcgg
     1921 cgcttcgtgg atgcgattgt tcgacgagcg ctatcgccaa aacaagtggc catacggctc
     1981 gggggtgtgg ggcaaacgcg aatgggtcat gcctcagatg cccgatgagt gcatcgtctc
     2041 gatgtacgaa ggcgggacga acctgttctg ggccgaccgc tacggcaaga cgctcggcct
     2101 caccgacttg tgggtgaagc tctgtggcaa cagccattcc gggtccttca aggacttggg
     2161 tatgaccgtg ctcgtcagcg tggtccgcat ggccatccgc gacggcctga aggtgaaggc
     2221 gatcgcgtgc gcttccaccg gcgacaccag cgcgtcgctc gcggcctacg gtgccgccgc
     2281 cggtctcccc gtcgtcgtgc tattgccgcg cggtaaaatc tcgaccgccc agctcgtgca
     2341 gccgctcgcg cacggcgcgt tggtgctcgg tctcgacacc gatttcgatg gctgcatgac
     2401 catcgtgcag cggctcgccg aagaaggctt ggtgtatctc gcgaactcca tgaacccgct
     2461 gcgcctcgaa ggtcagaaga cggtgggcat ggagatcgtc cagcagttcg actggcaggt
     2521 tcccgactgg atcatcttgc cgagcggcaa tctgggcaac gcctccgccc tctacgccgg
     2581 cttcaagatg ctgaaggagc tcggcgtcgt cgcgcgctac ccgcgcctcg tcgttgccca
     2641 ggcagaaaac gcgaacccgc tctaccgcgc ctggactcag ggcaagcgcg tggtcgaacc
     2701 gattcaagcg aagcccacgc tcgctaccgc gatccaaatc ggaaacccgg tga
//
LOCUS       DASTKS010000703         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_112648, whole genome shotgun sequence.
ACCESSION   DASTKS010000703 DASTKS010000000
VERSION     DASTKS010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112648"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFQ35_14515"
     CDS             complement(<1..641)
                     /locus_tag="VFQ35_14515"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET9931911.1"
                     /translation="MHLLINTGGGDAPGLNAVIRAVTLTGVRRGFRVTGVRRGYAGLL
                     ADFPSGLVPLNRDVVREIADRGGTILGTVNKGSPFEYPVANAHGEQELTDVSEKLIAN
                     FRATGADGLIAVGGDGSLRIAARLMEKGIPVIGVPKTIDNDLAATEATFGFDTAMSFA
                     TEAIGRLHSTAEAHSRVMVVEVMGRHAGWIALYSGIAGGANVILVPEMDFDFDA"
     gene            1059..2255
                     /locus_tag="VFQ35_14520"
     CDS             1059..2255
                     /locus_tag="VFQ35_14520"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HET9931912.1"
                     /translation="MTQIDSVLPKGISTVLLVDDEPAFLRALTRCLKSGPHAVEAFES
                     ATDAVERVRRGGIDVVVSDISMPGMNGVELLRQIRAHEPDLPVVLVTGLPALESATDA
                     VEYGAFKYLVKPVDPAALRATVDQAVQLYRLARMKRKALELIGIAGMASDRAGLEAGF
                     ERALSSLWVALQPILRSGDGSVFGYEALMRTREPSLPGPAEVLDAAERLSQLDRLGRV
                     VRARAVKPLLEARDDIVLFVNLHPEDLVDPELGAADSELASIASRVVLEITERASLEN
                     VENVRARVKALRELGFRIAIDDMGAGYAGLTSFALLEPDIVKLDMALVRGIDRKPVQQ
                     KLVASMTTLCKDMGLLVVAEGIETGQERDTLIELGCDLLQGFLFARPGPPFPVAEWHR
                     NALDDE"
     gene            complement(2271..2759)
                     /locus_tag="VFQ35_14525"
     CDS             complement(2271..2759)
                     /locus_tag="VFQ35_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9931913.1"
                     /translation="MNDDAHARSLEEREPRSERSTARKKHELALTARERSALLSLLSS
                     PDTTRNHARRVRVVLLSADGLRGVEIAQRLRLSPGQVSRIRARFAERRVEGLADEKHP
                     GRRDHAVSSEQVQAIERLSLSPPPEGRTRWSTRLIASRVGLTSATVAKVLRRTRAVDD
                     GG"
     gene            3074..4348
                     /gene="urtA"
                     /locus_tag="VFQ35_14530"
     CDS             3074..4348
                     /gene="urtA"
                     /locus_tag="VFQ35_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HET9931914.1"
                     /translation="MTLQAVPSRIGFAMSLLLFALSGALAGCKNAQGSTASSGSSAAS
                     AAPAAGAGKGLAITDSEVTVGQLHSATGTMAISETGSIQAERLAIDQINQSGGVLGKK
                     IKIIQEDGASDWPTFAEKAKKLLVQDRTASVFGCWTSASRKAVLPVFEKENGLLYYPT
                     FYEGLEQSKNVFYTGQEATQQILAGLDWLQKTKGAKSFFLIGSDYIWPRTSNKIARKH
                     IENVLKGSVVGEEYYPLGHTQFGSLINKIKAQKPDAVYAIVVGGSNVSFYKQLKAAGV
                     TAENQTLLTISVTEDELLGIGGENMVGFYAAMKYFQSLDNENNKKFVAAFKEKYGPKS
                     VIGDVTQAGYLGPWLWKAAVEKAGSFDVDKVVAASPGIELTTAPEGYVKIHPNHHLWS
                     RLRVGQVQADGQYKVVYESDLIEPNPFPKGYQ"
     gene            4370..5278
                     /gene="urtB"
                     /locus_tag="VFQ35_14535"
     CDS             4370..5278
                     /gene="urtB"
                     /locus_tag="VFQ35_14535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET9931915.1"
                     /translation="MDISFSIWAMQAFNGLSTFCVLLLMALGLAIIFGQMGVINMAHG
                     EFLTIGAYTTYLLSQASERLFPWMSSWYFLLAILVAFVLAGGVGLLVEHFMVRHLYKR
                     PLDTLLATWGLSLIMQQAFRSTFGAREVSATLPAWLMGSVKPTASIDIPINGLFVMAL
                     TLLLTGLVFFGLSYTRVGVNVRATMQNRAMASAAGIHTGRVDKLTFAIGCGLAGVAGA
                     AFTTIGSTGPTSGSLYIVDTFLVVVFGGAESLLGTIASAFGIAQTQATTEFFMTGSIA
                     KVLTLSLVIVILMVRPQGLFAVKVRR"
     gene            5283..>5470
                     /locus_tag="VFQ35_14540"
     CDS             5283..>5470
                     /locus_tag="VFQ35_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931916.1"
                     /translation="MKTLKQRLLTISGRFANTPDKRREVLGFLAMALLLLVVFPLGLD
                     TFRLNLVGKYLTYAFVALG"
BASE COUNT          954 a         1686 c         1791 g         1039 t
ORIGIN      
        1 gcgtcgaagt cgaagtccat ttcgggaacc aagatgacgt tggctccgcc cgcgatgccg
       61 gagtagagcg cgatccagcc agcgtggcgc cccatcacct ccaccaccat cacgcggctg
      121 tgggcttcgg cggtcgagtg aaggcgaccg atcgcctccg tggcgaagct catggccgta
      181 tcgaacccga acgttgcttc cgtcgcggcg aggtcgttgt cgatggtttt ggggacaccg
      241 attacgggaa ttcccttctc catcaggcgc gccgcgattc gtaagctgcc gtcgccgccg
      301 accgcgatga ggccgtcggc cccggtcgct cggaagtttg cgatgagttt ttcactgacg
      361 tcggtgagct cttgttcgcc gtgcgcgttc gcgacggggt actcgaaggg gctgcccttg
      421 ttcacggtgc cgaggatggt tccgcctcga tcggcgatct cgcgcacgac atcgcgattc
      481 agcggcacga gcccgctcgg aaagtcggcg agcagccctg cgtagccccg ccgcacgccg
      541 gttactcgaa agccccgccg aactcccgtg agcgttaccg cgcgaatcac ggcgttgagt
      601 cccggcgcgt ctccgccacc cgtattgatt agaaggtgca tcccctcgaa agctcgcctg
      661 aacgcgaaga gggcgtcaac tccctggcgg cgaaacccgc atggatgcgc gagaggagag
      721 gggttagtcg agttcgctcg gcgctccaag ccgaggcgcg taggtatttg gttggcggtc
      781 gcgttcggtg cgcgcggcgc gcctcgcgcg aaaagacgag cggaggggtg tcgggcaccg
      841 tcgtttcgtc cgtatggtgc ggaaagaggc gaagagcctc ggacaagacc gagtgggccg
      901 cgctgccttg cgtggttcgt cggcggtgtg ggaagatgaa agcttctgat tttatcttca
      961 ttgtctcttc ggtccgctcg cgaacaagtg ttcagcgcct ttgaagaagt cgtaacggtc
     1021 ttgggctccg cgttggctgc tcccattcga ggctttcggt gacgcaaatt gattcggtac
     1081 tgccgaaggg gatctccacc gtgctcttgg tggacgacga gcccgcgttc ttgcgggcgc
     1141 tgacccgttg cttgaaatcg ggaccgcacg ccgtcgaagc gttcgagagc gcgacggacg
     1201 cggtcgaacg cgtgaggcgg ggcggcatcg acgtcgtggt cagcgacatc agcatgccgg
     1261 ggatgaacgg cgtcgagctc ttgcgccaga ttcgtgctca cgagccagat ctacccgtcg
     1321 tgttggtgac gggcttgccg gcgctcgaga gcgcgacgga cgcggtcgag tacggcgcgt
     1381 tcaagtatct ggtcaagccg gtcgatccgg cggccctgcg cgcgaccgtc gaccaggccg
     1441 tgcagctcta tcggctcgcg cgcatgaagc gaaaggcgct cgagctcatc ggcatcgccg
     1501 gcatggcttc ggatcgggct ggcctcgagg cgggcttcga acgcgcgttg agctctctat
     1561 gggtcgcgct gcagccgatt ttacgctctg gcgacggctc cgtgttcggc tacgaggcgc
     1621 tcatgcggac gcgcgagcct tcgctgccgg gccccgctga agtgctcgat gcggccgagc
     1681 gcttgagtca actcgacagg ctcgggcgcg tcgtgcgggc gcgggcggtg aagcctctgc
     1741 tcgaggcgcg cgacgacatc gtcctgttcg tcaacttgca cccggaagac ctcgtggatc
     1801 ccgagctcgg cgcggccgac tccgagctcg ccagcatcgc ctcgcgcgtc gtgctcgaaa
     1861 tcaccgagcg cgcgtcgctc gagaacgtcg agaacgtgcg cgcgcgcgtg aaggccttgc
     1921 gagagctcgg tttccgcatc gccatcgacg acatgggcgc cggctacgct ggcctcacga
     1981 gcttcgcgct gctcgagccc gacatcgtga agctcgacat ggccctcgtt cggggcatcg
     2041 atcgaaagcc cgtgcagcaa aagctcgtcg cgtcgatgac cacgctctgc aaagacatgg
     2101 gcctgctcgt cgtcgcggaa gggatagaga ctgggcaaga gcgcgacacc ctgatcgagc
     2161 tcggctgtga tctcttgcaa gggttcttgt tcgcgcgccc gggaccgcct tttcccgtcg
     2221 ccgagtggca ccgaaacgcg ctcgacgacg agtgaatcgc ggcggcttcg tcagccgcca
     2281 tcgtcgacgg cccgcgtgcg tcgcaaaacc ttcgcaaccg tcgcgctcgt gaggcccacg
     2341 cgcgaggcga tgagccgagt ggaccagcgc gtcctacctt caggaggagg cgatagtgag
     2401 aggcgctcaa tcgcctgaac ctgctcgctg ctcacggcgt gatcacggcg cccggggtgc
     2461 ttctcgtcgg cgagcccctc gacccggcgc tccgcgaaac gcgcgcgaat gcgcgacacc
     2521 tgacccggcg acaagcggag tcgctgcgcg atttcgacgc cgcgcaggcc gtctgccgag
     2581 agcagcacga cacgcacacg ccgtgcgtgg ttgcgagtcg tatcggggct cgatagcaaa
     2641 gacagcagcg cgcttcgttc tcgcgcggtg agcgcgagct cgtgtttctt tcgcgccgtg
     2701 ctccgttcgc ttcgaggttc tcgttcttcg agtgaccgag cgtgcgcgtc gtcgttcacc
     2761 ccgcgagtat agacgcgaac gttgcgcgtt tctcgtatgc agttcgtgag cggtgtcgcg
     2821 cggacacagc atgtttcgtc cgtgatcgcg tctggtggcg cgaaacgaac gcgtaaagct
     2881 tcactcgtga gatttattcg ttggtcacga tcaccacaca cgagagaaac atgccgccgg
     2941 ctacaagcgg gtcacactga gattggatga gcgcgcgtag caatacacgg tgacgcgtgc
     3001 gcgattcgcg tgcggtgcgt cgtccgcgtg gcgagcggag ctttgctcgc gagcccatca
     3061 aaaggagcat gagatgactt tgcaggcagt gccttctcgc atcggattcg caatgagctt
     3121 gctgttgttc gcgctctcgg gagcgctcgc tggctgcaag aacgcccagg gcagcacggc
     3181 ctcgagcggg tcttctgcgg cttcggccgc gcccgccgcc ggcgccggaa aaggcctcgc
     3241 catcaccgac agcgaggtga cggtggggca gttgcacagc gctacgggca ccatggcgat
     3301 cagcgagacc ggatcgattc aggccgagcg cctcgcaatc gaccaaatca accaatccgg
     3361 cggcgtgctc ggaaagaaga tcaaaatcat tcaagaggac ggcgcgtcgg actggccgac
     3421 gttcgcagaa aaggcgaaaa agctgctggt tcaggaccgc accgcctcgg tattcggctg
     3481 ctggacctcg gcgtccagga aggcggtgct gcccgtcttc gagaaggaga acgggctcct
     3541 ctactatccg actttctacg aagggctcga gcagtccaag aacgtctttt acactgggca
     3601 agaggcgacc cagcaaattc tagccgggct cgattggcta cagaagacga agggcgcaaa
     3661 gagcttcttc ctgatcggct cggactacat ttggccgagg acctcgaaca agatcgcgcg
     3721 caagcacatc gaaaacgtgc tgaagggtag tgtcgtcggt gaagagtact atcccctcgg
     3781 acacactcag ttcggctcgc tgatcaacaa gatcaaggcg caaaagcccg acgccgttta
     3841 cgcgatcgtc gtcggtggca gcaacgtctc gttctacaag cagctcaagg ctgccggcgt
     3901 caccgccgag aaccagacgc tgctcaccat ttcggtcacc gaagacgagc ttctcggcat
     3961 cggcggcgag aacatggtcg gcttctacgc ggcgatgaag tacttccaga gcctcgataa
     4021 cgagaacaac aagaagttcg tcgccgcctt caaagagaaa tacgggccga aatcggtgat
     4081 cggcgacgtg actcaggcgg gctacctcgg tccctggctc tggaaagccg cggtcgaaaa
     4141 ggcgggcagc ttcgacgtcg acaaggtggt cgccgcgtct ccgggcatcg agctcacgac
     4201 cgcgcccgaa ggatacgtca agattcaccc gaaccaccac ttgtggagcc gcttgcgcgt
     4261 cgggcaggtg caggccgacg gacagtacaa ggtcgtctac gagtcggact tgatcgagcc
     4321 gaaccccttc cccaagggtt atcagtgagc gtcgcgaaag aggtgtccca tggatatctc
     4381 cttttcgatt tgggcgatgc aggccttcaa cggcctcagc accttctgcg tgctcctgct
     4441 catggcgctc gggctcgcga tcatcttcgg gcagatgggc gtgatcaaca tggcgcacgg
     4501 ggagttcctc accatcggcg cgtacacgac ctacctgctg tctcaagcga gcgagcggtt
     4561 gtttccctgg atgagcagtt ggtacttcct gctggcgatc ctcgttgcat tcgtgttggc
     4621 gggcggcgtg ggccttttgg tggagcactt catggtgcgt cacctctaca aaaggccgct
     4681 cgacacgctg ctcgccacgt ggggcctgag cctcatcatg cagcaggcgt tccgctccac
     4741 gttcggcgct cgggaggtca gcgcgacctt gccagcgtgg ctgatgggct cggtgaagcc
     4801 gaccgcgagc atcgatatcc ccatcaacgg cctgttcgtg atggcgttga cgctgctgct
     4861 cacggggctc gtcttcttcg gcctctcgta cacgcgggtc ggcgtcaacg tgcgggcgac
     4921 gatgcagaat cgagccatgg cgagcgcagc cggcattcac accggccgtg tcgacaagct
     4981 caccttcgcg atcggttgtg ggctcgccgg ggtcgcgggc gccgcgttca cgaccatcgg
     5041 ctcgacgggg ccgaccagcg gctcgctcta catcgtcgac acgttcttgg tggtcgtctt
     5101 cggtggtgcg gagagcctac tcgggacgat cgcgtcggcc ttcggcatcg cgcagacgca
     5161 ggcaacgacc gagtttttca tgaccggctc gattgcgaag gtgctcacgc tgtcgctcgt
     5221 gatagtgatt ctgatggtgc ggccccaggg gcttttcgcc gtcaaggtgc ggaggtaact
     5281 gcatgaagac gctgaaacag cgcctgctca ccatttctgg gagattcgcg aacacacccg
     5341 acaagcggcg agaggtgctc gggtttctcg cgatggcgct tttgctgctc gtggtgttcc
     5401 cgctcggcct cgatacgttt cgcttgaact tggtgggcaa gtacctgacg tatgcgttcg
     5461 tcgcgctcgg
//
LOCUS       DASTKS010000704         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_781287, whole genome shotgun sequence.
ACCESSION   DASTKS010000704 DASTKS010000000
VERSION     DASTKS010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781287"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1070
                     /locus_tag="VFQ35_14545"
     CDS             291..1070
                     /locus_tag="VFQ35_14545"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9931917.1"
                     /translation="MDSELQASPDASTPSIQPAELPPRPAGSPSVAAAGAVGALLGAL
                     LVVAFGDAVWSGKTDASKLSLDIEAAATAPRIAPANDVLAALGTVALDPEHVATVTIP
                     GPGVVRHVASFEGDRVRAGSLLAVIQSPNANRDPTARYELLSPIEGTVIERSLHPSDD
                     VEPEVLAFRVANLDHLKVDLRLKKSDARHLRVGDRAELSPLAAHELFVDGSVAAISER
                     TPEESTVSVRVSDPHHYLREGDGVRAHLYSAAAGGDRATLL"
     gene            complement(1087..1578)
                     /locus_tag="VFQ35_14550"
     CDS             complement(1087..1578)
                     /locus_tag="VFQ35_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9931918.1"
                     /translation="MVSIQPGDEAIEDVELESLLRRVYVDGGFTDPAVAEQALKPNAV
                     RRRGELLAAVTDDGSVAGIVIAVLPGSPARRIASESEAEMHLLAVLPEYRGLGIGSRL
                     VEAAVGRAKAAGLTKMVLWTQPSMLEAQRLYVAHGFVRRAERDASLSPPAGRSFWVYE
                     RLL"
     gene            complement(1733..2260)
                     /locus_tag="VFQ35_14555"
     CDS             complement(1733..2260)
                     /locus_tag="VFQ35_14555"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9931919.1"
                     /translation="MTTYKVDAAHSEIAFTVRHMMFAKVRGQFTKWTAELEYDADDPT
                     KSKARAEIDTTSIDTREAARDGHLRSADFFDTEHFPKMVFVSKSVEPAGKGRYKVLGD
                     LTIRDQTHEVSLEVEETGRGRDPWGNDRIGFSAKGSLSREKWGLKWNQALEAGGVLVS
                     DKVEIDVEAQVVQSK"
     gene            complement(2384..3304)
                     /locus_tag="VFQ35_14560"
     CDS             complement(2384..3304)
                     /locus_tag="VFQ35_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9931920.1"
                     /translation="MFDPVTLDQLRALVAVAEEGSFSGAARKLQRVQSAISTAMANLE
                     EQLAVPIWDRSTKVPRLTDEGRAVLAAARRVLSEVDSLRRLTTGMASGLEASVSLCVD
                     ALFPLGVLVDLCVSFAKEFPTVDLRVDTQVLSAVSARVLEGAATIGVAGEHGLAQGLA
                     RKVLAPIRMVPVVGATHPLASVRGPVATAQLSDTVQVVLSERSETGVADQGVLSPRTW
                     RVADLYTKHALLRAGLGWGNLPEHLVSDDLAKGRLVRVQLAEWGDAEQVLLLCAIYRG
                     DATFGPAHRWLLAQLETACAGAPTPKKARR"
     gene            3412..>4019
                     /locus_tag="VFQ35_14565"
     CDS             3412..>4019
                     /locus_tag="VFQ35_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9931921.1"
                     /translation="MIRIRPANERGHADHGWLDSHHTFSFADYYDPNHMGFRALRVIN
                     DDTVEAGRGFGTHPHRDMEIISYVLDGALQHKDSMGTGSIIRPNDVQRMSAGTGVTHS
                     EFNASQSESVHFLQIWLLPERRGIKPSYEQKTFSEDDKSGRLRLVASPDGREGSTTVH
                     TDALVYAGLFDGEQRAELPLSPNRHAWVQVARGKVSVNGHSLS"
BASE COUNT          743 a         1390 c         1214 g          672 t
ORIGIN      
        1 ggcagtcatc gtggcccgca gtgtgaagaa gggcagcacg acgcttcagc tgcgcgacga
       61 gcaggcaaat ccactctggt cgcactagct agaacgccga tcagtctgac tgctcggcgc
      121 cgtttgtcgc tatagcaggg cgttgaaaaa cgccctgcta gggctcgttt cccttcgagc
      181 ccgaacggcg gaaaacgccg aagtctggaa gcacgaagtc ttcccgaaaa ctcagtggaa
      241 ccgcgtcgac gcgagtccat gcttagtggt caactgaagg acggtgcaac atggactccg
      301 agcttcaggc gtcgcccgac gcgtccacgc cgagtattca gcccgctgag ttgcccccgc
      361 gcccggccgg ttcgcccagc gtggcggccg cgggtgcggt tggagctcta ctcggagcgc
      421 ttctggtcgt ggccttcggc gatgccgtat ggagcggcaa gaccgacgcc tcgaagcttt
      481 ctctcgatat cgaagccgct gcaactgcgc ccaggatcgc cccggcgaac gatgtgctcg
      541 cagcgctcgg caccgtcgcg ctggatcccg agcacgtggc cacggtgacc atccccgggc
      601 ccggagtcgt gcggcacgtg gcgagcttcg aaggggatcg ggtgcgcgcg gggagcttgc
      661 tcgcggtgat ccaatcgccg aacgccaatc gcgatccgac cgctcgctac gagcttttgt
      721 cgccgatcga aggcaccgtg atcgagcgct cgctgcatcc gagcgacgac gtcgagcccg
      781 aggtcctggc gttccgggtc gccaacctcg accacctcaa ggtcgatttg cgactgaaaa
      841 agagcgacgc gcgtcatctg cgggtcggtg atcgcgccga gctctcaccg ctcgccgcgc
      901 acgagctgtt cgtcgacggg agtgtggccg caatctcgga gcgcactccg gaagagagca
      961 ccgtgagcgt ccgcgtctcg gacccccacc actacttgcg cgaaggcgac ggggtacgcg
     1021 cgcacctata ttctgccgct gcgggcggcg atcgcgccac gctgctctag accctcgtca
     1081 cccgactcac agcaggcgct cgtagaccca aaagctgcgc ccagccggcg gcgagagcga
     1141 agcgtctcgc tccgcccgcc gaacgaagcc gtgagcgacg tacagccgct gagcctccag
     1201 catgctgggt tgggtccaga gaaccatctt ggtaagcccc gccgctttcg cgcggccgac
     1261 tgcggcttcg actagacgag acccgatgcc gagcccgcga tactccggaa gcaccgcgag
     1321 caagtgcatc tcggcctcgc tctcgctcgc aatccgacgc gccggcgagc ccgggagtac
     1381 cgcgatcacg atgccagcga ccgacccgtc gtcggtgacc gccgcgagga gctcgccgcg
     1441 ccgtcgcacc gcgttcggtt tcagcgcctg ctccgctacg gccgggtcgg tgaagccgcc
     1501 gtcgacgtaa acacgtcgca acagcgactc gagctcgacg tcttcgatgg cttcatcgcc
     1561 cggctggatc gagaccacga acgcttcata gcccagcggc agggtgctcg ctcaaattct
     1621 ccgaaactcg agcagagcgt gaccgactag gaaactgcca ggcggctccg gtaccagctc
     1681 tcccctgacc ctcccctccc aggggccgtc cggatcgcct ggcgattcaa cttcacttgc
     1741 tctgcacgac ctgagcttcg acgtcgatct ccaccttgtc gctgacgagc acgccgcccg
     1801 cctcgagcgc ctggttccac ttcaagcccc acttctcgcg gctcaggctg cccttggcag
     1861 aaaacccgat gcgatcgttc ccccacggat cgcgcccgcg gccggtctct tcgacctcca
     1921 gcgacacctc gtgggtttgg tcgcggatcg tgagatcccc caacaccttg tagcggccct
     1981 tgccagcggg ttcgaccgac ttgctcacga acaccatctt cgggaagtgc tccgtatcga
     2041 agaaatccgc gctgcgcagg tgcccgtcgc gcgcagcctc ccgcgtgtcg atgctcgtgg
     2101 tgtcgatctc ggcgcgcgcc ttcgatttgg tggggtcgtc cgcgtcatac tcgagctcgg
     2161 ccgtccactt ggtgaactgg ccgcgtacct tcgcgaacat catgtggcga acggtgaaag
     2221 cgatctcgga gtgagcggcg tcgactttgt acgtggtcat ggggaacctc ctcggtagtc
     2281 gatgaatcta cgcgccccga cccatcctga aaatcgatga aaaattgatt caaatcatct
     2341 gcaaatcaga tggtttgccg gtgagcgctc ggggcggact cgctcaccga cgcgcctttt
     2401 tgggggtggg agcgcccgcg caagccgttt cgagctgcgc caagagccac cgatgagccg
     2461 gcccgaaagt cgcgtcaccg cgataaatcg cgcaaagcag gaggacctgc tccgcatcgc
     2521 cccactccgc cagttgcacc cgaacgaggc gccccttcgc cagatcgtcg ctgacgaggt
     2581 gctcggggag gtttccccaa cccaggccag cgcgtaagag cgcatgcttc gtgtatagat
     2641 ctgcgacgcg ccaggtgcgg ggcgagagca cgccttgatc tgcaaccccg gtctcgctgc
     2701 gctccgacaa aaccacttgg accgtatcgc tcagctgtgc cgtcgcaaca ggtccgcgca
     2761 cggacgcaag gggatgcgtc gcgcccacga cgggcaccat gcgaatcgga gccagcacct
     2821 tgcgcgcaag gccctgagca agcccgtgct cgccggcgac tccgatggtc gccgccccct
     2881 cgagcacgcg cgcactcacc gccgacagca cctgggtatc gacccgaaga tcgacggtag
     2941 gaaactcctt cgcaaagctc acgcagagat cgaccaacac cccgagcggg aacagcgcat
     3001 cgacgcacag agaaaccgac gcctcgagac cgctcgccat acccgtggtc agccgccgca
     3061 acgaatcgac ctcgctgagc actcgacgcg ctgcggcgag gacagcgcgc ccttcgtccg
     3121 tcagcctggg cactttggtc gagcggtccc aaatcggaac cgcaagctgc tcttcgaggt
     3181 tcgccatggc cgtgctgatg gccgactgca cgcgctgcag cttgcgcgcg gcgcccgaaa
     3241 agctcccttc ctccgcgacc gcgacgagag cgcgcagttg gtcgagcgtg acgggatcga
     3301 acatggcctt caccatctct aaaccagatg gttactgtca accgattgcc gatttacgcg
     3361 atggctggca cgacccaagc tgagtcgtcg aacccacaga ggagcccgaa catgatccgc
     3421 atccgtcccg ccaacgaacg agggcacgcc gaccacggct ggctcgacag tcaccacacc
     3481 ttctctttcg ccgactacta cgatccgaac cacatgggct tccgcgcgct gcgcgtcatc
     3541 aacgacgaca ccgtcgaagc cgggcgcggc tttggcacgc acccacaccg cgacatggag
     3601 atcatctcgt acgtgctcga tggcgctcta cagcacaagg attcgatggg taccggctcg
     3661 atcattcgcc cgaacgacgt gcagcgcatg agcgctggga ccggcgtcac ccacagtgaa
     3721 ttcaacgctt cgcagagcga aagcgtgcac tttctccaga tttggctctt gcccgaacga
     3781 cgcggcatca agccgagcta cgaacaaaag accttctccg aagacgacaa gtcggggcga
     3841 ctccgcctgg ttgcctctcc ggatggacgc gaaggcagca cgaccgtgca caccgacgcc
     3901 ctggtctacg ctggcctctt cgatggcgag caacgtgcgg agttgccgct ttcgccgaac
     3961 cgccatgcgt gggtgcaggt cgctcgcggt aaggtttcgg tgaatgggca tagcctctc
//
LOCUS       DASTKS010000705         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_559044, whole genome shotgun sequence.
ACCESSION   DASTKS010000705 DASTKS010000000
VERSION     DASTKS010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559044"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..627
                     /locus_tag="VFQ35_14570"
     CDS             247..627
                     /locus_tag="VFQ35_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931922.1"
                     /translation="MTRRRKEDDSRPRREARAPLPPRDEAPKPSLDESGMTHSSLIEA
                     LRAIEGLVDVGRDPPNFQFCSRPFLHFHEQDGRVYADVRFGRDFEPVWASTPGERSEL
                     FSRVAEHVERVASTRKAKRRPRRA"
     gene            complement(692..1150)
                     /locus_tag="VFQ35_14575"
     CDS             complement(692..1150)
                     /locus_tag="VFQ35_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9931923.1"
                     /translation="MAATSPAWFFVLVIVRDGDRFLVVQERKFGQTWYLPAGRVEVGE
                     DFVAAAQRETLEEAGIHIEVNGIIRIEHTPNLNGERRIRIMFVASPVGSLAPKRVADE
                     HSLGAAWVTLDELKTLPLRGEEVVGLFEHVARGGAVHPLSLLTYEGAPYA"
     gene            1234..>2154
                     /locus_tag="VFQ35_14580"
     CDS             1234..>2154
                     /locus_tag="VFQ35_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931924.1"
                     /translation="MFRAAPFAFWAPTAAWVAGCGEAKGNATASSTGGTAAAGGSSAS
                     GGSGFTSLGGGGSSAGGATQTAGANNGLNGGTSAANGGSVSTSGGVATSSGGTSTANG
                     GSAASPGGGYAATGNGGSSASTGGVAASNGGTSQGTALGGANVGGTATGGEQSRGGAN
                     TGGFGGGLSPVGCPPNASFCANFETVALPTGAIYKSTGASTNWLDDFEIDHIVSAIGR
                     GCLKVKPTTEAQSGSAYQMLAAPSGGSVFWARFHIRADQVLGQDAHNVFVAASAGDGP
                     NDAALVELAEDCGLALKGRDNVLRPDGSSQN"
BASE COUNT          392 a          688 c          733 g          341 t
ORIGIN      
        1 acgttgccga gcggcctccc tacgagcgcc tcgcgtagat cctcgtagag cacgtcgcgc
       61 cagatgcgat cgccgcaccg gaatggctca gacgtacggc gcgccttcgt acgtgagcag
      121 cgaaagcggt gaacagcgcc accgcccgcg acgtgttcga acgagaagca ctttttcttg
      181 ggcgctcgcg gcgtccatca tagatgcagc cggggtctgc aaggcgccag ctcttagggg
      241 gctcgaatga cgcgacgtcg aaaagaagac gatagccgtc cgcgccgcga ggcgcgtgct
      301 ccattgccgc cacgggacga agcacccaag ccgtcgctgg atgagagcgg catgacgcac
      361 tcgtcgctga tcgaggcgct acgagcaatc gaaggtctgg tggatgttgg gcgtgaccct
      421 cccaactttc aattttgttc gcggccgttc ctccactttc acgagcaaga tggccgcgtg
      481 tacgccgacg ttcggttcgg gcgtgatttc gagcccgtct gggcgtcgac tccaggtgaa
      541 cgcagcgagc tcttcagtcg cgtcgccgaa cacgtggagc gcgtcgcgtc cacgcgcaaa
      601 gcgaaacgtc gcccgcgtcg cgcctgaggt gccgggcatc ttgtctagct ggtgcgagcg
      661 gtatggatcg ccgcacagcg ctcgaccgca ctcacgcgta cggcgcgcct tcgtacgtga
      721 gcagcgaaag cggatgaaca gcgccaccgc gcgcgacgtg ctcgaacaag cccacgacct
      781 cttctccgcg caacggaaga gttttcaact cgtcgagggt gacccacgcc gcgccgagcg
      841 agtgctcgtc ggcaactcgt ttgggagcga gcgagccgac cggcgacgca acgaacatga
      901 tgcggatgcg ccgctctcca ttgaggttcg gcgtgtgctc gatgcggatg atgccgttta
      961 cctcgatgtg aataccagcc tcctcgagcg tctcgcgctg cgcggctgcg acgaagtcct
     1021 cgcccacttc gacgcgcccc gccggcaagt accaggtctg accgaacttg cgctcttgaa
     1081 cgaccaggaa ccggtcgccg tcgcgaacga tcaccaacac gaaaaaccag gccggtgacg
     1141 tagccgccat gcccctgagc ttactccgtg tttgacagcg gccggaaagt cggccacgaa
     1201 cgatccgtgg acaggtggtg gcataagggg ctgttgttca gggctgcgcc gttcgccttt
     1261 tgggcgccaa cggccgcttg ggttgcggga tgcggcgaag ccaaggggaa cgcaaccgct
     1321 tcgagcacgg gcggcacggc cgcggcgggc ggctcctcag ctagcggggg ctccggcttc
     1381 acctcgctcg gcggcggagg gagctccgct ggcggcgcaa cccagaccgc gggtgcaaac
     1441 aacggactca atggaggcac ctccgccgcg aatggcggga gcgtgtccac gagcggcggc
     1501 gtcgcaacca gtagcggggg cacctcgacg gcaaacggcg ggagcgcggc gtccccgggc
     1561 ggcggatacg ccgcgacggg aaacggagga agctccgcct ctacgggcgg cgtcgcagcg
     1621 agcaacggag ggacctctca aggcacggcg ctcggtggag caaacgtggg tgggaccgcg
     1681 actggaggag aacaatcgag aggcggcgca aacaccggcg ggttcggggg cgggttgtcg
     1741 cccgtcggtt gtccgccgaa cgcttccttc tgcgcgaact tcgagacggt cgcgctgccc
     1801 accggagcaa tttacaagag caccggcgct tcgacgaatt ggctcgacga tttcgagatc
     1861 gaccacattg tgtctgcaat cggcaggggc tgcctcaaag tcaagccgac tacggaggct
     1921 caatcgggca gtgcgtatca aatgctcgcg gctccgtccg gcggcagcgt gttctgggct
     1981 cgctttcaca tccgtgctga ccaagtgttg ggacaggacg cgcacaacgt tttcgtcgcc
     2041 gcgtcggcgg gcgacggccc gaacgacgcc gcgttggtcg aactcgcgga agactgcggg
     2101 ctcgcgctga agggccggga caacgtgctg cgccccgacg gctcgagtca gaac
//
LOCUS       DASTKS010000706         6170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1229280, whole genome shotgun sequence.
ACCESSION   DASTKS010000706 DASTKS010000000
VERSION     DASTKS010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229280"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /gene="atpA"
                     /locus_tag="VFQ35_14585"
     CDS             complement(<1..1617)
                     /gene="atpA"
                     /locus_tag="VFQ35_14585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9931925.1"
                     /translation="MQLSADEISSIIKKQIQNFDRASLVSETGTVLATGDGIVRAYGL
                     ESALAGELVEFSGGTYGLVLNLEQDNVGIAILGDPTSVREGDVVKRTGRIADVPVGEA
                     VVGRVVNALGQPIDGKGPIESKHRRRIEVKAPGIIVRQTVREPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKGKGVTCIYVAVGQKASTVAQVVERLNQYGAM
                     EYTIVVAATATEPAPLQFLAAYTGVTMGEYFRDTGRHALCIYDDLSKQAVAYRQMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKMSERWFVVKKGTQVPPGDVNFRGVDGQEHVG
                     SECRKKVAKALEARPDKAELEVQKDPWSGGSLTALPIIETQAGDVSAYIPTNVISITD
                     GQIFLESDLFYSGIRPAINVGISVSRVGGNAQSKAMRKVAGTLRLELAQFREMAAFAQ
                     FASDLDAATRAQLERGRRLTELLKQGQFVPLPLERQVVTIFAGTNGYTDKLPVESLKA
                     YEEELYRFLDEKHPDIFNDIREKAELTDDIR"
     gene            complement(1680..2222)
                     /gene="atpH"
                     /locus_tag="VFQ35_14590"
     CDS             complement(1680..2222)
                     /gene="atpH"
                     /locus_tag="VFQ35_14590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9931926.1"
                     /translation="MSHSTVAERYGRAIFELGVESGSLESLVDRIQQFAKAYETSADL
                     RQVLENPLIDPARRDAILNDVAVRLNLSGLALNSVKLLARRRRLNMLPEIASLLSELS
                     DERAGVVRATVTTAVPMGESFYERLKAELETMTQRRVVIERRQDPSLIAGVVTRIFDN
                     TIDGSLKGRLSDMERQLRAS"
     gene            complement(2219..3019)
                     /locus_tag="VFQ35_14595"
     CDS             complement(2219..3019)
                     /locus_tag="VFQ35_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET9931927.1"
                     /translation="MKALRRWLVSSSLAGALALAGAAAVAAQDHGQNAAEHAVDHAVQ
                     HAAAESEDTAPPHGHGAEHEQHHVPTFDDVNWFYGILAARDGVEPSLLFRPTGMPVPV
                     ASLFLNAFIVYYLLYRLLGKTIADGLRKRKETILRGMQEAAKMKAEAEAELAQYEERL
                     AKIEQEIERIRREMRQTAETERNRVLTEAKERRERMERDARELVEQELAAVRQALFSE
                     TVQSAMRSAETALKERVTSADQQRLADEYIAGLRQTRVSLRPSTRGRA"
     gene            complement(3020..3511)
                     /locus_tag="VFQ35_14600"
     CDS             complement(3020..3511)
                     /locus_tag="VFQ35_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET9931928.1"
                     /translation="MTSIDLAVGSVTGLVAMSGGVNLDFDNTVILQAALFTTLLLVLK
                     PLLFDPVLKVFALREERTEGARATARELQERAGELLGQYEQELSRVAQVAAEERERLR
                     AETARLEAEILLEARTAAANIIEQGRKRIETEMNRVRFDMGRESEKVSGLIVSRVLGR
                     EGN"
     gene            complement(3714..4247)
                     /gene="folK"
                     /locus_tag="VFQ35_14605"
     CDS             complement(3714..4247)
                     /gene="folK"
                     /locus_tag="VFQ35_14605"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9931929.1"
                     /translation="MSEISARSDEICTPLDAVVGLGSNLGDRFSYLRFAVRRLAELGA
                     DLRVSGIYETEPLGPPQPRYLNAAVRARFELSPRSLLNSLLEIERAAGRERRERWGAR
                     TLDLDLLWIAGVSVNEPDLRVPHPELTRRAFALAPLVELAPEATDPQADESYSERLAR
                     LDLEGVERLSVALWERG"
     gene            4424..>6170
                     /locus_tag="VFQ35_14610"
     CDS             4424..>6170
                     /locus_tag="VFQ35_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931930.1"
                     /translation="MVSEPTPSADTGNGPSSEALIFVSDASAEAERITSGLRVRGYLT
                     VDVPLGLLVGRVAVQPPALILCDVDAPQALETIERMRALPSTSNIDVLFIGEAGRTLD
                     EQAERVGVQGSGIFVRPVDVGALTRRIETLLGPPKTTGARLSSPPQASRVPVLVAATR
                     KPYRYDARGKASRPSFESIPPPPLPSEPPHSNSQWPSSPTASGETRPGLSARTSLDPY
                     AGFDTRAGGEGRAGHDLLENADPYAISPDLAQLLGRAERRVLESKHFAAHGERLSPEA
                     ELEAILPEDLLSALDEPLDELDGDDDDPGTPGTHGESSFGARSRSSHDSQGGGTSPGG
                     AAPAPAARESTTSGGDRVDEIAQDKPAPPAEPVRDMAPATPQASRHQRTSPPSLTHTG
                     VDTGFGSIPPAPALPSEALPANAASPPSSERDSSPAQRHRRSTRPPRPRPPRELGEDS
                     AAELEPGPPPRPAITGPHAQGSTYGTGPASSSRNGARAERTAPPLDRNERGASPPERA
                     ERVEIEIPNTLRGGDALRVLARAVRARYSGAIAFEDNAGIRRLVLRDGDFVIAAAGIE
                     PESLLNFLVQRGDLSA"
BASE COUNT         1048 a         2100 c         1949 g         1073 t
ORIGIN      
        1 acggatatcg tccgtgagct cggccttctc gcggatgtcg ttgaagatgt ccgggtgctt
       61 ctcgtcgagg aagcgataca gctcttcttc gtaggccttg agcgactcga ccggcagctt
      121 gtcggtgtag ccgttggtgc cggcgaagat cgtgaccacc tggcgttcga gcggcagcgg
      181 cacgaactgg ccttgcttca agagctcggt caagcgacgg ccgcgctcga gctgagcccg
      241 ggtcgccgcg tcgaggtcgc tcgcgaactg agcgaaggct gccatctcgc gaaactgcgc
      301 gagctcgagg cgcagcgtgc cggccacctt acgcatggcc ttgctctgcg cgttaccacc
      361 cacgcggctg acgctgatgc cgacgttgat ggcggggcgg atgcccgagt agaacagatc
      421 gctttcgagg aagatctgac cgtcggtgat cgagatcacg ttggtcggaa tgtaagcgga
      481 cacgtcaccg gcctgggtct cgatgatcgg caacgccgtc agcgaaccac ccgaccacgg
      541 atccttttga acctcgagtt cggccttgtc ggggcgggcc tcgagcgcct tggcgacctt
      601 cttgcggcac tcgctaccca cgtgctcttg gccgtcgacg ccgcggaagt tgacgtcgcc
      661 gggcggaacc tgggtgccct tcttcaccac gaaccaacgc tcggacatct tggccgcgcg
      721 ctcgagcagg cgagagtgga gatagaacac gtcaccgggg tacgcctcgc gacctggcgg
      781 acggcggagc aagagggaca tctggcggta agccacggct tgcttcgaca agtcgtcgta
      841 gatgcacagc gcgtgacggc ccgtgtcgcg gaagtactcg cccatcgtca cgccggtgta
      901 cgccgcaagg aattgcagcg gagccggctc ggtcgcggtc gccgcaacca cgatggtgta
      961 ttccatcgcg ccgtactgat tgaggcgctc caccacctgc gccacggtcg aagccttctg
     1021 gcccaccgcg acgtaaatgc aggtcacacc cttccccttt tggttgagga tggtgtcgat
     1081 cgcgatggtc gtcttgccgg tctggcggtc accgatgatg agctcgcgct gaccgcggcc
     1141 aatcgggatc atcgagtcaa tcgctttgat gcccgtctgc agcggctcac gcaccgtttg
     1201 acgaacgatg ataccgggag ccttgacctc gatgcggcgt cggtgcttcg actcgatcgg
     1261 gcccttgccg tcgatcggct gaccgagcgc gttcaccacg cgacccacga cggcctcgcc
     1321 gaccggcacg tccgcgatgc ggccagtacg cttgaccacg tcgccttcgc gcacgctggt
     1381 cgggtcgccc agaatggcga tacccacgtt gtcctgctcg aggttgagca ccaagccgta
     1441 ggtgccgccc gagaactcga ccaactcacc tgcgagcgca gattccaggc cgtatgcgcg
     1501 cacgatgccg tcgcccgtgg cgagcaccgt cccggtctcg ctcaccagag acgcgcggtc
     1561 gaagttttga atctgcttct taataatcga ggaaatttcg tcggcgctga gctgcatgac
     1621 ggtctctcac aagctaggaa ccacgattcc accggcgggt gtgaatcaac acagtttctt
     1681 caagaagcac gcagctgtcg ctccatgtcc gacagccggc ccttcaacga accatcgatc
     1741 gtgttgtcga aaatacgggt aaccacgccc gcgatcaggg acgggtcctg acggcgctcg
     1801 atcacgaccc ggcgttgggt catggtttcg agctcggcct tgaggcgttc atagaacgac
     1861 tcgcccatcg gcacggccgt cgtcacggtg gcgcgtacca cgcccgcccg ctcgtcagag
     1921 agctcggaca gcagcgaggc gatctcgggc aacatgttca aacggcgtcg gcgcgcgagc
     1981 agctttacgc tgttcaacgc caagccgctc aggttcaagc gcaccgccac gtcattgagg
     2041 atggcgtcac ggcgagccgg gtcgatcaag gggttctcga gtacctgccg caagtcggcg
     2101 ctggtctcgt aggctttggc aaactgctgg atgcggtcga ccaggctctc gagtgaaccc
     2161 gactcgacgc cgagctcgaa gatcgctcgg ccataacgct ccgcaacggt ggagtgactc
     2221 atgctcgccc tcgcgtgctg ggccgcagac tcacgcgagt ctgacgcaaa ccagcgatgt
     2281 attcatcggc caggcgctgc tggtccgcag acgtaacgcg ctccttgagc gccgtctccg
     2341 cagagcgcat cgcgctctgc accgtttccg agaacaaggc ctggcgcacc gccgcgagct
     2401 cttgctccac gagctcgcgc gcgtcgcgtt ccatgcgctc gcgtcgttct ttggcctcgg
     2461 tcagcacgcg attgcgctcg gtttcggcag tttgtcgcat ctcgcggcga atacgctcga
     2521 tttcctgctc gatcttggcg agccgctctt cgtactgggc gagctcggct tcggcctcgg
     2581 ccttcatctt cgcggcttct tgcatgccgc gaaggatggt ttccttgcgc ttgcgcaggc
     2641 cgtccgcgat cgtcttcccg agcagacgat acagcaggta atagacgatg aaggcgttca
     2701 ggaagagcga cgccaccggc acgggcatac ccgttggccg gaacagcagg ctcggctcga
     2761 ctccatcacg cgcggcgaga atgccgtaga accagttgac gtcgtcgaac gtcgggacgt
     2821 gatgctgctc gtgttccgcg ccgtgaccgt gcggaggcgc cgtatcttcg gactcggcgg
     2881 ccgcgtgttg aacagcgtgg tcgaccgcgt gctcagccgc gttctgaccg tgatcctggg
     2941 cggcaacggc ggcggcgccg gccagggcca gcgcaccggc caggctggat gaaaccagcc
     3001 aacgtcgaag agctttcatt cagttgccct ctcgtccgag cacacgggac acgatcaatc
     3061 cggaaacctt ctcggattct cgccccatgt cgaaccgcac gcggttcatc tcggtttcaa
     3121 tccgcttgcg cccctgctcg atgatgttcg cggccgccgt gcgcgcctcg agcagaatct
     3181 cggcttccag ccgagccgtc tccgcgcgga ggcgttcacg ctcttcggcg gcgacctgcg
     3241 ccacgcgcga cagctcctgt tcgtactgac cgagcagctc accggcgcgc tcctgaagtt
     3301 cgcgggccgt ggctcgggca ccctcggtgc gttcctctct caaggcaaac accttgagca
     3361 ccggatcgaa aagcaaaggt ttcaacacca agagcagcgt cgtgaagagc gccgcctgca
     3421 agatcacggt gttgtcgaaa tccagattga ccccgccact catcgcgacg aggcccgtga
     3481 cgcttcccac ggcaaggtcg atcgaagtca ttgctggcta tgcctactga tggctatgcc
     3541 tctgctggct ctgcctgttt cgcctgttgg agccgttgac cttctgagcg ctccctctgg
     3601 aggctgcttt caaggtcgct ggcagtgcac gaaaattcct gcatcgccaa gcggcggggc
     3661 ggctcgtacc atcgtgccgt cggcgtgtcc acagcggcgt ttaggtttgt gccctaaccg
     3721 cgctcccaaa gtgccacgga caggcgctct acgccctcta gatcgagcct cgctaggcgc
     3781 tcggaatacg actcgtcggc ctgagggtca gtggcttccg gcgccagctc gacgagcggc
     3841 gccaaagcga acgctcgcct cgtcaactcg gggtgtggca cgcgcaggtc cggctcgttc
     3901 acgctgaccc ccgcgatcca caggaggtcg agatccaaag tgcgcgcacc ccagcgctcg
     3961 cgccgctcac gcccggcggc ccgttcgatt tccaaaagcg agttcaataa ggagcgcgga
     4021 gagagctcga agcgggcgcg caccgccgcg ttcaagtaac ggggttgtgg gggcccgagc
     4081 ggctccgtct cgtaaatgcc ggaaactcgc aggtcggcac ctaactcggc cagccgccgc
     4141 accgcgaagc gcaggtagga gaagcggtcc cccaggttgg agccgagccc aaccacggcg
     4201 tcgagtggcg tgcagatttc gtcggaacgg gcagagatct cggacacgag tcggatatca
     4261 taacccggtg ctcggcctca ccggctcgga actagctctc gtgtctttca tcgtcgtcgc
     4321 catcgtctcg gcgcggcact ggccagccat gggcgagcgg ctagccctga tgctttggcg
     4381 cgagccaaag gggccaagcc gcgagggcta agaggctttc gcggtggttt ccgaacccac
     4441 gcccagcgct gacaccggaa acggaccgag ctccgaggcg ctgatcttcg tcagcgatgc
     4501 ctcggcggaa gcggagcgca ttacctccgg cttgcgggtt cggggctact tgacggtcga
     4561 cgttccactt ggccttttgg ttggccgagt cgcggttcag cccccggcgc tgattctatg
     4621 tgacgtcgat gcgccgcaag cgctcgagac gatcgagcga atgcgggcgc tcccgagcac
     4681 ctcgaacatc gatgtgctgt tcatcgggga agcggggcgc acgctggacg aacaggccga
     4741 gagagtcggc gttcagggta gcgggatctt cgttcgcccc gtagacgtcg gcgcgctcac
     4801 gcggcggatc gaaactctgc tcggcccacc caagacgacg ggcgcgcggc tatcgtctcc
     4861 accccaagcc agccgcgtac cggtgctggt cgcagccacg cgcaagccat atcgctatga
     4921 tgcgcggggc aaagcttcgc gccccagctt cgaatccatc ccgccgccac ccctccccag
     4981 cgagccgccg cactcgaact cgcaatggcc gagcagccct actgcgagcg gcgagactcg
     5041 tccgggcctc agcgcgcgca ccagcttgga tccgtacgcc gggttcgata cgcgcgcagg
     5101 cggcgagggc cgcgccggcc atgacctgct cgagaacgcg gatccgtacg ccattagccc
     5161 agacctcgcg cagttactgg gtcgcgccga gcggcgcgta ctcgagtcca agcacttcgc
     5221 ggcgcacggc gagcggcttt cgcccgaggc cgagctcgag gcgatcttgc cggaagattt
     5281 actgtccgcg ctcgacgagc cgctcgacga gctcgatggg gacgacgatg atcccggcac
     5341 ccccggcacc catggggaat cgagcttcgg cgcacgcagc cgctcgagcc acgattcaca
     5401 aggaggaggc acctcgccgg gtggcgccgc gccagcaccc gcagcgcgtg agtccacgac
     5461 ttccggtggt gatcgcgtcg acgagattgc tcaagacaaa cccgcccccc ccgccgagcc
     5521 ggtgcgcgac atggccccgg ccacgcccca ggccagtcgt caccaacgca cttcaccgcc
     5581 cagcctcacc cacacgggtg tagacactgg cttcggcagt attccccctg cgcccgcgct
     5641 gccttccgag gcattgccgg ccaacgccgc gtcgcccccg tcttcggagc gcgattcctc
     5701 tcccgcacaa cgtcaccgtc gctcgacgcg gcctcctcgc ccacggcctc cgcgtgagct
     5761 cggggaagac tcggctgccg agctcgaacc ggggccgccc ccacgccccg cgatcacggg
     5821 tccccacgcc cagggcagta catacggaac gggccccgcg agctcatcgc gcaacggcgc
     5881 acgcgccgag cggaccgcac cgccgctcga ccgcaacgaa cgcggagcga gcccgccaga
     5941 gcgcgccgag cgcgtcgaaa tcgagatccc gaacacgttg cgcggcggag acgcgctgcg
     6001 cgtcttggcg cgcgccgttc gcgcccgcta ctcgggggcg atcgctttcg aagacaacgc
     6061 gggcatccga cgcttggtgc tgcgcgacgg tgatttcgtg atcgccgcgg ccggtatcga
     6121 gccagaatcc ctgctgaatt tcctggtgca acgcggtgat ctcagcgcgg
//
LOCUS       DASTKS010000707         9411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_782122, whole genome shotgun sequence.
ACCESSION   DASTKS010000707 DASTKS010000000
VERSION     DASTKS010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782122"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="ssb"
                     /locus_tag="VFQ35_14615"
     CDS             <1..448
                     /gene="ssb"
                     /locus_tag="VFQ35_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9931931.1"
                     /translation="GADPELRMTPNGQAVLKLRLATSESYLDRNKVRQERTEWHSVVV
                     WGKRAEALNKILSKGSRIFVEGGLRTSSYDDREGNKRYRTEIVASNLILAGGARGGGG
                     GGMRGGDDYGGPPEGGAPSGGGGGGGGGGYDDADYGGGGGDDDIPF"
     gene            470..2152
                     /locus_tag="VFQ35_14620"
     CDS             470..2152
                     /locus_tag="VFQ35_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931932.1"
                     /translation="MTSSRRLGRRRVLRGGTLLVAGLLSVTPLTLSSCRKAPPAPLTK
                     PAPLPDRPPEVVAELVLPTPHELWPRFRTLAGPIGDVLPSSPELALAAIASVPPLGAG
                     SLDVRSPIAIAWLGGDAPGLVVGVHVESGPELVAQVASGSHPTHRVDRRGGVAYLSQA
                     SGAGFSFAVVSDTLLLGPERAVERAGEYVARALRPRAPTQGPVVVDFIGPSLKAWLVP
                     RLSAAWAEQRAALVEALGREQSRKGRPADLADPAALLGALDGVVRDALGVVGSARGAH
                     AEISTASERLTLSLRVEADGALRALLAALRPGPADRLRELPPGAALALFRRRTSDTST
                     NGFVRALFGPRLSAADAARLDRALDRLRAGRGDAEAFALMPDLGLVWRGDVNDATELR
                     AAFADLVPFLARPPFADALSESVGRPSLGRRPVERRGDDTVERSLIRMMPKSGGPSAA
                     KNYELTTFVGTKRFVAAFGKSSSTVADAAIAAEAAPEARLGGAPPFASQLSATGDVAW
                     LLVTDLTRLGLAPADVNAVASIEAGVGDGALTVTLRATDGAVRALAAREFGR"
     gene            2149..3240
                     /locus_tag="VFQ35_14625"
     CDS             2149..3240
                     /locus_tag="VFQ35_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9931933.1"
                     /translation="MNGVLLVTGFPALRARHIVRALLTRDAAVRVVVLVARERADEAR
                     AVLSREALAERVSLVEGDPTAIDFGLGGARYLELARQVRIVHAAYSITDAAPERIAEA
                     VNVGQARELVELVRVAPELRAIVLYSSVFVSGQRSGIVKEAELEAGQGVRGPVERTLA
                     TAEKMLRDSHVPRIVLRAGHLLGDTAEGEVEHLTGPYWLLAFIASASKDAPLPVLPGA
                     DAPLCITPVDYLARFGAFAGERAAPGSTFHVIDPARLTLRQFLAEVAERSGRRLEPGF
                     HPSSLTRLLAGNPALKGLPQQVRSLLEVLTGSAEYASDGALDLVNHGAPECPPFASYL
                     DRLFDHVRARLERGDLFSSRRQSPAFLVA"
     gene            3191..4498
                     /locus_tag="VFQ35_14630"
     CDS             3191..4498
                     /locus_tag="VFQ35_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9931934.1"
                     /translation="MTSSRRVASHRRFSWRSLRRAVVAVVTLGSLFEAVIGPPPARAD
                     APVPAASTPLSVGAGAEPSDAARTPELRHFYDALAALARHQRNESVRVLWLGDSHTAS
                     DFLTGAVRSRLVERYGSGGPGFLRLGVPVRHDLATFERSGRFRIQPESPATRLHQDDG
                     VFGFGGMRVTPLSDGARLGVRLAPRSVRGRAKFTLLYDVKLGGEFAMRLGSTAVNVRG
                     TEGAQRVAGSPVLRRSIEGEPSEPFSVEVRRGRPRFYGLFVDGSEPGVVVDTVGINGA
                     RVATALAWAEAPFAAEVLAREPELVVMAFGTNEAFDELRVSAYEAQLAQLLRRLRQRP
                     NVDCLVLGPPDALAPSGEPAARVAEITRVYASSARTLGCAFVSAQALMGGPGAFASWQ
                     RQDPQLASADRVHLTPKGYRKLGELVFDALFAGAAPAEATNAP"
     gene            complement(4420..5886)
                     /locus_tag="VFQ35_14635"
     CDS             complement(4420..5886)
                     /locus_tag="VFQ35_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931935.1"
                     /translation="MLEKSPPAEPWRAVFLSRPFGAALLVTAFAVAAELVPALAPIRV
                     LGGSAEQQATTLDAEPQNLTPPEVEAKALAPPASSAATPAARIGFSDPPPKVPLESAN
                     PRALDDFFRALDATRAKKPGAVTHIIHFGDSVVASDYVSGTLRRLLQQRFGDAGHGFT
                     LIMPAWPGYFHNDIETYSTSGWKLSRVVMPFADDGLYGLGCVSFQAEKNTLARVATNK
                     KSDFGRNVSRFGVSYLAGPEGGTFQLSVDGAPQGLIDTRAISKEARVHTLDVPDGPHE
                     FELLTKSGTTRLFGFLLERDVPGVVLDAIGVQGARVRFLDKQDDAHWAEQLRSRNPAL
                     LVYQFGANESADGLMYPMVDYHRTMKDVLEQGQRAVPHASCLVIGVMDRASKKGDEIR
                     SLSIIPRLLAEQRAVAAEVGCAFFDTYRAMGGAGSMPSWVKRGLGQADLTHPSSVGAD
                     MIGTWIFRALMERYGAFVASAGAAPANSASNTSSPSLR"
     gene            complement(5903..7306)
                     /locus_tag="VFQ35_14640"
     CDS             complement(5903..7306)
                     /locus_tag="VFQ35_14640"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9931936.1"
                     /translation="MWKLTIEDDQTNKTVVHLVRDEYGLGRAEENAIRLTERNISRLH
                     AQLERKNEHWALRDLSSYNGCYVNGQRVAGAQELNHGDVIQVGDYRLALENEALLVNE
                     QDATATVPAPRTAAPSSSVDRLVMLMGPSPGAEFELTQQRMVIGRGEDCDVALNHASV
                     SRIHAEMHPLGDGRYEIVDRQSANGVRVNGVELPRSFIDARDVIELGDVILKFIPAGQ
                     MYTPGADESLQIAAIGAARRQEAEESGIEAFGRTSNAKWFVGLGVLFLVAVAAAVVIT
                     QRSTPAEMVSMKDAATARAEQTLSAAKQLLAQGDPTGAYAKSSEVPQGSALRETSDFR
                     QIQAAYADYLFTQADQASDAADKRALYDQIARAPTIDRGRRNRAAEQLAALSAKAVNV
                     KDLPNARPPAPPLDEAQRARTTPAPSASEEAPEVPEPPPAAAPPPRTPTPRGERSTPP
                     PATSKGQATTLVRESPF"
     gene            complement(7395..8549)
                     /locus_tag="VFQ35_14645"
     CDS             complement(7395..8549)
                     /locus_tag="VFQ35_14645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9931937.1"
                     /translation="MPVLLLAAFSRTSALDDLAERAVIDAGVARAAALGVAVRAGGEW
                     SVEVGAAGKLGRTIRASELTPFDLASVSKSFVACCFARLWKRGVLQPQTELESLVPEC
                     RGTRSARRSLAALLSHRAGLEGHRRLFAPLERGRPIERTTALRTAANAERRDVSNTLE
                     PPPLYSDLGYLLAGEALARAAGCALDQLVENEVSRPLGLGAGSARQWLRREPDFRNLV
                     APTEDVAFRGGTLTGVVHDENAWAFAGHGLAGQAGLFGTVLDVLGFGTALLDALSGRN
                     EGWLERAALEFLIAPRPGGTLRLGFDGKSDEHSSAGPGASTRTFGHLGFTGTSCWCDP
                     SAEAVTVLLTNRVCPTRENLRIRACRPIVHEALFERARHLRRAYGPGEHG"
     gene            8671..>9411
                     /locus_tag="VFQ35_14650"
     CDS             8671..>9411
                     /locus_tag="VFQ35_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9931938.1"
                     /translation="MAEFTWEARARTGELRKGSMEADSEEAVNQRLRQQQLNPVKVAR
                     KRDFGSLSFGSGVSVKDLVTFTRLFATMIDAGLPLVQCLEILGSQQTNKHFAAVLKDI
                     RGAVEGGSTFSEALRRHPKVFDELFVNLTHAGEVGGILDSILNRLSIYLEKRQKLVRQ
                     VRGALVYPSIVVVIAAGVMTVLLTFVIPAFEGMFKEFGGGKDAMPKLTQLVMDFSHLF
                     VSALPLLIVFGILASVGFTYFYRTPRDRK"
BASE COUNT         1375 a         3103 c         3280 g         1653 t
ORIGIN      
        1 gggagccgac cccgagcttc ggatgacccc gaacgggcag gcagtgctga agcttcggct
       61 agcgaccagc gagagctacc tcgatcgcaa caaagtgcgc caggagcgaa ccgagtggca
      121 cagcgtcgtc gtttggggca agcgcgccga ggccctgaac aagatcctct cgaaaggttc
      181 ccgcatcttc gtcgaaggcg ggctgcgcac ttcgagctac gacgatcgtg aggggaacaa
      241 gcggtatcgg actgaaatcg tcgcgagcaa cctcatcttg gcgggtggcg cgcgcggcgg
      301 tggcggcggc ggtatgcgcg gcggcgacga ctacggcggt ccgcctgaag gcggtgctcc
      361 gtctgggggc ggaggcggtg gcggcggtgg cggctacgac gacgctgatt acggcggcgg
      421 cggcggcgac gacgacatcc cgttctgaaa tcgtcctcgc gagtcgagga tgacgtcgtc
      481 tcgaaggctt gggcgccgcc gtgtattgcg cggcggcacc ctgctcgtcg cagggctttt
      541 gagtgtcaca cccctgacgc tctcttcctg tcgcaaggct ccgcccgcgc cgctcacgaa
      601 gcccgcgccg ttaccggatc gcccgcctga agtcgtcgcc gaactcgtct tgccgacgcc
      661 gcacgagctt tggccgaggt tccggacctt ggcagggccc atcggcgatg tcttgccgag
      721 cagcccagag ctcgcgctcg cggccatcgc gtcggttcct cctctcggcg cgggctcgct
      781 cgacgtgcga tcaccgattg cgatcgcctg gctcggcggt gacgcgccgg gcctggtggt
      841 cggcgtgcac gtcgagagtg gcccggagct cgtcgctcaa gttgcttcgg gatctcatcc
      901 gactcatcgc gtcgaccgtc gcggaggagt cgcgtacctg tcgcaagcct ccggggcagg
      961 gttttcgttt gcggtcgttt cggacacgct gctgctcggt cccgagcgag cggtcgagcg
     1021 ggccggtgag tacgtggcgc gagcgctgcg ccctcgagcg cccacgcagg ggccggtcgt
     1081 cgtcgacttc atagggccct cgctcaaggc ttggctcgtt ccgcggttga gcgcggcctg
     1141 ggcagagcag cgagcggcgc tcgtcgaggc actcgggcgc gagcagagtc gcaaggggcg
     1201 ccccgccgac ttggcggatc cggcggcgct cctcggcgcg ctcgacggtg tagttcgaga
     1261 tgcgctcggc gtggtcggca gtgctcgcgg cgcgcacgca gagatttcca ccgccagcga
     1321 gcgcttgacc ttgtcactcc gcgtcgaggc agatggcgct ctacgcgcgc tgctcgcggc
     1381 gctgcggccg gggccagctg accgacttcg agaactgccg ccgggcgcgg ctctcgcgct
     1441 ctttcggcgt cgcacgagcg acacctcgac gaatggcttc gtgcgcgcgt tgtttggacc
     1501 tcgcttgtcc gccgcggacg ccgcgcggct cgaccgcgcg ctcgaccggc tgcgggccgg
     1561 acgcggggac gcggaagcgt tcgctttgat gcccgatctc ggcttagtgt ggcgcggcga
     1621 cgtgaacgac gcgacagaac ttcgagccgc tttcgcggat ttggtgccgt tcctggctcg
     1681 gccgccgttc gcagatgcgc tgagcgagag cgtaggtcgg ccgtcgctcg ggcggcgtcc
     1741 cgtcgagcgg cgcggggacg acacggtgga gcgctcgttg attcgtatga tgccgaagag
     1801 cggggggccg agcgcggcga agaactacga gctgacgacg ttcgtcggaa cgaagcgctt
     1861 cgtggccgcg ttcggcaagt ccagttcgac cgtggctgat gccgcgatcg ccgcggaagc
     1921 agcacccgag gctcggctcg gcggagcgcc tcccttcgcc tcgcaattgt cggctacggg
     1981 cgacgtcgct tggttgctcg tgaccgactt gaccaggctc ggcttggctc cggccgacgt
     2041 gaacgcggtc gcgagcatcg aggcaggggt cggcgatggc gcgttgacgg taacgctccg
     2101 agcgaccgac ggcgccgtgc gcgcgctcgc ggcacgggag ttcggacgat gaacggggtc
     2161 ttgctcgtta cgggctttcc cgccctgcgc gcgcgtcaca tcgttcgtgc gctgctgacc
     2221 cgtgacgcgg ccgtgcgggt cgtggtgctg gttgcgcggg agcgcgccga cgaagcgcgc
     2281 gcagtcctct cgcgagaagc gctcgccgaa cgcgtttcgc tcgtggaagg cgatccaacg
     2341 gcgattgact tcgggctcgg gggcgcgcgg tacttggagc tcgcacgtca ggttcgcatc
     2401 gtgcacgccg cctactcgat caccgacgcg gcgccggagc gcatcgccga ggccgtcaac
     2461 gtcgggcagg cccgggagct cgtcgagctc gtgcgtgtgg cgccggagct ccgcgcgatc
     2521 gtgctctatt cgagtgtgtt cgtcagcggt cagcgctcgg gtatcgtcaa agaggccgag
     2581 ctcgaggcag ggcagggcgt gcgtggcccc gtcgagcgca cgctcgcgac cgcggagaag
     2641 atgctgcgtg acagccacgt cccgcgcatc gtgctgcgcg ccgggcacct gctcggcgat
     2701 accgcggagg gtgaagtcga gcacctcacc gggccgtatt ggctgctcgc tttcatcgcc
     2761 agcgccagca aagacgcgcc gctaccggtg ctgcccggcg ccgacgcccc gctctgcatc
     2821 actcccgtgg attacttggc gcgcttcggc gcgttcgccg gagagcgcgc ggcgcccggc
     2881 agcacgtttc acgtcatcga cccggcgcgg ctcaccctgc gtcaattttt ggcggaggtc
     2941 gccgagcgct ccgggcggcg actcgagccc ggtttccacc cgtcctcgct cacgcggctc
     3001 ctagctggga accccgctct caagggtttg ccgcagcaag tcaggagcct tttggaggtg
     3061 ctcaccggga gcgcagagta cgctagcgac ggcgccctcg atctcgtaaa ccatggagcg
     3121 ccggagtgcc ccccatttgc gagctacctc gaccgactct tcgaccacgt gcgagctcgg
     3181 ctcgagcgcg gtgacctctt ctcgtcgcgt cgccagtcac cggcgtttct cgtggcgtag
     3241 cttgcggcgc gcggttgtcg cggtcgttac gctgggctcg ctgttcgagg cagttatagg
     3301 tccgcctccg gcgcgcgccg acgctcccgt tcctgccgcc tcgactccgc tctcggtagg
     3361 ggcgggcgcg gagccttccg acgccgcgcg gacgcccgag ctccgacact tctacgatgc
     3421 gctcgctgcc ctcgccaggc atcagcgaaa tgagtcggtg cgggtgctct ggctcggaga
     3481 ctcgcacacc gcgtcggatt ttctcaccgg cgcggttcgg tcgcgcctcg tcgaacgcta
     3541 cggttccggc ggtcccggct tcctgcgctt gggggttccc gtgcggcacg atctggcgac
     3601 gttcgagcgc agcgggcgct ttcgcattca gccggagtcg cccgcgactc gcttgcatca
     3661 ggacgatggc gtcttcggct tcggtgggat gcgcgtgacg cccctgtcgg acggtgcgcg
     3721 cctcggggtg cgcctcgctc cgcgctccgt ccgcggccgc gccaagttca cgttgctcta
     3781 cgacgtgaag ctcggtggcg agttcgcgat gcgtctcggc tcgaccgcgg tgaacgtgcg
     3841 cggcaccgaa ggcgctcagc gtgtcgctgg gagtcccgtc ctgcgacgca gcatcgaagg
     3901 ggagcctagt gaaccgttct cggtcgaggt gcggcgcggg cggcctcgat tttacgggct
     3961 cttcgtcgac ggcagcgaac cgggcgtcgt cgtcgacacg gtgggcatca acggcgcgcg
     4021 cgtcgcaacc gcgctcgcct gggccgaggc gccgttcgcc gccgaagtgc tggcgcgcga
     4081 gccagagctc gtcgtgatgg cgtttggaac gaacgaggcg ttcgacgagc tgcgggtgag
     4141 cgcctacgag gcgcagctcg cccagctctt gcgtcgcctg cgccagcggc caaacgtcga
     4201 ttgtctagtg ctcggtccgc cggatgcgtt ggcaccgagc ggtgagcccg ccgcgcgcgt
     4261 cgccgaaatc actcgggtgt acgcttcgag cgcgcgcacc ctcgggtgcg ctttcgtgag
     4321 cgcacaagcg ttgatgggcg gtcccggcgc attcgcaagc tggcagaggc aggatccgca
     4381 gctcgcgagc gcggatcgcg tccacctcac gcccaagggc tatcgcaagc tcggtgagct
     4441 cgtgttcgac gcgctgttcg ccggcgccgc gcccgccgag gccacgaacg cgccgtagcg
     4501 ctccatcagc gcacggaaaa tccaagtccc aatcatgtcg gcaccgaccg agctcggatg
     4561 cgtcaagtcg gcttgcccaa ggcctcgttt gacccagctc ggcatcgagc cggcgccgcc
     4621 catggcccgg taggtatcga agaatgcgca cccgacttcg gcggcgacgg cgcgctgctc
     4681 ggcgagcagc cgtgggatga tgctgaggct ccggatctcg tcgcccttct tgctcgcgcg
     4741 atccatgaca ccgatgacga ggcagctcgc gtgcgggacg gcgcgctggc cctgctcgag
     4801 cacgtccttc atggtgcggt ggtaatccac catcgggtac atgaggccgt cggcgctctc
     4861 gttcgctccg aactgataga cgaggagcgc gggattgcgg ctcctcagct gttcggccca
     4921 gtgcgcgtcg tcctgcttgt cgaggaagcg cacgcgagcg ccttgcactc caatcgcgtc
     4981 gagcacgacg ccaggtacgt cgcgttccaa gagaaaaccg aagagccgcg tcgttccgct
     5041 cttcgtaagc agctcgaact cgtgtgggcc gtccggaacg tcgagcgtgt gtacccgcgc
     5101 ctccttgctg atggcgcgcg tgtcgatcaa gccttgcggg gcgccgtcga ccgagagctg
     5161 gaacgtgccg ccctcgggac ccgctagata cgagacgccg aagcgcgata cgttgcggcc
     5221 aaaatcgctc ttcttgttgg ttgcgacccg cgcgagcgtg ttcttctcgg cttggaacga
     5281 aacgcagcca aggccgtaga gcccgtcgtc ggcgaacggc atgacgacgc ggctgagctt
     5341 ccatcccgac gtcgaataag tctcgatgtc attgtgaaag tagcctggcc acgccggcat
     5401 gatgagggtg aagccgtgac ccgcgtctcc gaagcgctgt tgcagcaacc gacgtagcgt
     5461 gccggacacg tagtcgctcg cgacgaccga gtccccgaag tgaatgatgt gcgttaccgc
     5521 tcccggcttc ttcgcgcggg tcgcgtcgag cgcgcggaag aagtcgtcga gcgcgcgcgg
     5581 attggcgctt tcgagcggca ccttgggggg cggatccgaa aagccgatgc gcgcggccgg
     5641 cgtcgccgcg gagctcgctg gcggggccaa cgctttcgct tcgacttccg gcggcgtcag
     5701 gttttgtggt tcggcatcga gcgtcgtcgc ctgctgctct gccgaaccgc cgagcacgcg
     5761 gatgggcgcg agcgcgggga ccaattcggc ggctacggcg aaggccgtga ccaggagcgc
     5821 cgcaccgaac ggtcgactca aaaagacggc tcgccagggc tcggcgggcg gagatttttc
     5881 gagcacgggc tccacgcgcg cgtcagaagg gcgattcgcg cacgagggtc gtcgcctggc
     5941 ccttcgacgt ggcgggtggc ggggtggagc gctcgccacg cggagtcggc gtgcggggcg
     6001 gcggcgcggc ggcgggcggc ggctccggaa cctcgggcgc ctcttcgctc gcggacggcg
     6061 cgggagtggt ccgagcgcgt tgggcctcgt ccaagggcgg ggcggggggg cgcgcgttcg
     6121 gaaggtcttt gacgttgacc gctttggcag agagcgcagc gagttgctcg gcagcgcgat
     6181 tgcggcggcc gcgatcgatg gtcggggcgc gcgcgatttg atcgtagagg gcgcgcttgt
     6241 cggcggcgtc actcgcctga tccgcctgcg tgaagaggta atccgcgtac gcggcctgga
     6301 tttgacgaaa atcggaggtt tctcggagcg cggagccttg cggcacctcg ctcgactttg
     6361 catacgcgcc ggtcggatcg ccctgagcca aaagctgctt cgccgcgctg agcgtctgct
     6421 ccgcgcgcgc ggtcgcggca tccttcatgc tcaccatctc tgccggcgtc gagcgctggg
     6481 tgatgacgac ggccgcagcg actgccacta ggaagaggac gccgagcccg acgaaccact
     6541 tggcgttcga ggtgcgaccg aacgcttcga tgccgctctc ttcggcttct tgtcggcgtg
     6601 cggcgccgat cgcggcaatt tgcaggctct cgtccgcgcc cggcgtgtac atctgcccgg
     6661 cgggaatgaa cttcaggatg acgtcgccga gctcgatgac gtcgcgcgcg tcgatgaagc
     6721 tgcgaggcag ctcgacgccg ttgacgcgca cgccgttcgc gctctgccgg tcgacgatct
     6781 cgtaacgacc gtcgccgagc gggtgcatct cggcgtgaat acgactgacg gacgcatgat
     6841 tgagggcgac gtcgcagtct tcgccgcgcc caatcaccat gcgctgctgc gtgagctcga
     6901 actccgcgcc cgggctcggc cccatgagca tcaccaaccg atcgaccgag gagctcggcg
     6961 cggcggtgcg cggcgcgggg acggtcgccg tcgcgtcctg ttcattcacg agcaacgcct
     7021 cgttttccaa cgcgaggcgg tagtccccca cttggatcac gtcgccgtgg ttcaactcct
     7081 gtgcgcccgc tacgcgctgc ccgttgacgt aacagccgtt gtagctggac aggtcacgca
     7141 gggcccagtg ctcgtttttg cgttcgagct gcgcgtgaag gcgcgagatg ttgcgctccg
     7201 tcagacgaat cgcattctct tcggctcgac ctagcccgta ttcgtcgcga acgagatgca
     7261 cgaccgtctt gttggtctgg tcatcctcga tcgtcagctt ccacatggtt cgtatcgtgc
     7321 cagtgtagcc tggcccgccg cgcgcgaacg ctcacgaatt tcgcatgcgc tcagggcttc
     7381 gggaagcgtt ttgatcagcc gtgctcgcca ggcccatacg ctcttctaag gtgtctcgcg
     7441 cgttcgaaga gcgcctcgtg gacgattggc cggcacgctc ggatgcgcag gttttcgcgc
     7501 gtggggcaca cgcgattcgt aagcagcacc gttacggcct cggccgaagg gtcgcaccag
     7561 cagctcgtgc cagtgaagcc gaggtgtcca aaggtgcgtg tgctggcacc ggggccggcc
     7621 gatgaatgtt cgtccgactt gccgtcgaag ccgagtcgta gcgttccacc cgggcgtgga
     7681 gcgatgagga actcgagggc ggcgcgttcg agccagccct cgtttcgccc agacagggca
     7741 tcgagcagcg ctgttccgaa gccgagaaca tcgagcacgg tgccgaacaa tcccgcttgc
     7801 ccagcgagcc catgccccgc gaaagcccag gcgttctcgt cgtgaacgac gccggtcaag
     7861 gtgccaccgc ggaaggcgac gtcttcggtt ggcgctacga ggtttcgaaa gtcgggctcg
     7921 cgtcgcagcc actgtcgcgc cgagccggcg ccgagcccga gcggcctcga aacttcgttc
     7981 tccacgagct ggtcgagcgc gcagcctgca gcccgtgcga gcgcttctcc cgccagcagg
     8041 tatccgaggt cgctgtagag cggtggcggt tcgagcgtat tcgatacatc gcgccgctcg
     8101 gcgttcgccg ccgttcgtag cgctgtagtc cgttcgatgg gacgtccccg ctcgagcggc
     8161 gcaaagagcc tgcggtgccc ctcgagccct gcccgatgcg agaggagggc tgcgagcgag
     8221 cggcgagcgc tgcgcgttcc gcggcactcc gggacgagac tttctagctc cgtctgcggt
     8281 tgcaggacgc cccgcttcca caagcgcgcg aagcagcacg cgacgaacga tttcgagacc
     8341 gacgcgaggt cgaacggtgt cagttcgctc gcgcggatcg ttcgccccag ttttccggcc
     8401 gcacccacct ccacgctcca ctccccaccg gctcgcaccg cgactccgag cgctgccgct
     8461 cgagcgacgc cggcatcgat gaccgccctt tcggctagat catcgagcgc agaggttcgg
     8521 gagaaggccg ccaaaaggag gaccggcatc gtggcctcgc tcaccggacg aggcgaactt
     8581 ttgaccgacg ctttgcgaga gggctattct ggggcttgcc ccgaagcggg cggaagcgct
     8641 tgattccaat gcgaacctcg gacccgtcgg atggctgaat tcacctggga agcccgggct
     8701 cgcaccggcg agctgcgcaa gggttcgatg gaggctgaca gcgaagaggc tgtcaatcag
     8761 cgccttcgtc agcagcaact gaaccccgtc aaggtggcgc gcaagcgcga cttcgggagc
     8821 ctcagcttcg gcagcggggt ctccgtcaaa gacctcgtta ccttcacgcg cctcttcgcg
     8881 acgatgatcg atgcgggcct accgctcgtt cagtgcctgg agattctggg tagccagcaa
     8941 acgaacaaac acttcgcggc cgtgctgaag gacattcgcg gcgcggtaga aggcggctcg
     9001 accttcagcg aagcgctgcg ccgccacccc aaggttttcg acgagctctt cgtcaacttg
     9061 acccacgccg gtgaagtcgg cggcatcttg gacagcatcc tgaaccgctt gtcgatttac
     9121 ctcgagaagc ggcagaagct ggtccggcag gtgcgcggcg ctctcgtcta cccgtcgatc
     9181 gtcgtggtga tcgcggcggg cgtgatgacg gtgctgctca cgttcgtcat tcccgcgttc
     9241 gaggggatgt tcaaggagtt cggcggcggt aaagacgcga tgccgaagct cacgcagctc
     9301 gtgatggact tctcgcacct gttcgtctcg gcgctgccgt tgctcatcgt gttcggcatc
     9361 ctggcgagcg tcggcttcac gtacttctac cgaacgccac gagatcggaa g
//
LOCUS       DASTKS010000708         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1229685, whole genome shotgun sequence.
ACCESSION   DASTKS010000708 DASTKS010000000
VERSION     DASTKS010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229685"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1546)
                     /locus_tag="VFQ35_14655"
     CDS             complement(86..1546)
                     /locus_tag="VFQ35_14655"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HET9931939.1"
                     /translation="MMNAEILPNPSLVAPAERSERAPIPAVSRALLAEGLVEREGVLT
                     GTLPPWLNGRLLRTAPALFNAGTFHAKHWFDGIGMLYGFRFRGGKATFAQRLLECEAA
                     AEAHRGRLPRATFASPNQRSFWRRIFEPIPEVTDNANVNVLRQGDEWVALTESPYQLR
                     VSDAELSVRGRVVYDDALPKNMGMTAHPHFDFARNVAVNLGTVLGRKGELIAFEQAQG
                     SATRREIGRYVANELPYQHSFGITAASVVIVGHPWLVRPTSLLWSNRGFAEHFRWKPE
                     LGTRFIVLDRGGGATREFSAEALFTFHTVNTYPDGDDLVLDLLAYPDPSIVQNLKVEN
                     MLDGIVGLRPRLCRAHLGANGRARLETLSELGFEFPSINYRRVSGVRHRFVWGATARG
                     DGRDIVGIDCESGVVRHFGVPGFVFGEPIFVAAPGARAEDEGVLLAVGSNGESSKLAV
                     LDAHSLEPEALLHLDLPIPLGFHGSFSRDGGDRVAA"
     gene            1658..>2109
                     /locus_tag="VFQ35_14660"
     CDS             1658..>2109
                     /locus_tag="VFQ35_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET9931940.1"
                     /translation="MSFLPAASILAVLEGLAALKLDVARIRREVFRDEGESNPEALVP
                     AALFGEVLRAAQMQAPREELLLEAALSVPLGAFGLMDYLAASSDTIGEAFVSLSRLFP
                     SVAPGCAIEVDESPREYVVTLRNPNEHSLRVNGDELTVGVMLRHFRNLS"
BASE COUNT          384 a          682 c          685 g          358 t
ORIGIN      
        1 gcaagcagag cgaaaccgat atcgaaattt gcgaccgaaa aatcacgcgg gtagttagac
       61 gcggctcgcc cttcgccgca agcttttacg ccgccacccg gtcgcccccg tcccgtgaaa
      121 acgagccgtg gaagccgagc gggatgggca agtcgaggtg caagagggcc tcgggttcga
      181 ggctatgcgc gtcgagcacg gcaagcttcg aactttcgcc gttggagccc acggcgagca
      241 ggacgccttc gtcttcggcc cttgcccccg gcgcggcgac gaagatgggc tcgccaaaga
      301 cgaagcccgg cacgccgaag tgacgaacca cgccgctctc acaatcgatg cccacgatgt
      361 cgcggccgtc gccgcgcgcg gtcgcgcccc agacgaagcg gtgccgcacg ccgctcacgc
      421 gccggtagtt gatgctgggg aactcgaagc cgagctcgct cagagtctcg aggcgtgccc
      481 gtccgttcgc tcccaggtga gcgcggcaga gtcgcggacg cagaccgacg atgccgtcga
      541 gcatgttctc gaccttcagg ttttgaacga tgctcggatc cgggtaagcc aagagatcta
      601 ggacgaggtc gtctccgtcc ggatacgtgt tcacggtatg gaaggtgaac aacgcttcgg
      661 cgctgaactc gcgggtcgcg ccaccgccgc gatcgagcac gatgaagcga gtaccgagct
      721 cgggcttcca gcgaaaatgc tccgcgaagc cgcgattgga ccaaaggagt gaggttgggc
      781 gcacgagcca ggggtgcccc acgatgacga cgctcgcggc cgtgatcccg aacgagtgct
      841 ggtaggggag ctcgttcgcg acatagcgcc cgatctcgcg tcgcgtcgcg ctgccctgag
      901 cctgctcgaa cgcgatgagc tctccctttc gcccgagcac ggtaccgaga tttaccgcga
      961 cgttccgcgc gaagtcgaag tgtgggtgcg ccgtcatgcc catgttcttc ggcaacgcat
     1021 cgtcgtacac gacgcgacct cgcacgctga gttcggcgtc gctcacgcga agctggtacg
     1081 gcgactcggt gagagcgacc cactcgtcac cctgccgcag aacgttcacg ttcgcgttgt
     1141 cggtgacctc ggggatcggc tcgaaaatgc gtcgccaaaa ggagcgctga ttcggtgacg
     1201 cgaaggtcgc gcgcgggagc cggccgcggt gcgcttcggc cgccgcttcg cactcgagca
     1261 agcgttgagc gaaagtcgcc ttcccacccc ggaaacgaaa gccgtacagc atgccgatgc
     1321 cgtcgaacca atgtttcgcg tggaaggtgc ccgcgttgaa gagcgcaggc gccgtgcgca
     1381 atagacggcc gttgagccag ggcggcagcg tgcccgtcaa aaccccttcg cgttcgacga
     1441 gcccttccgc cagcaaggcg cgcgatacgg ctggaatcgg cgctcgttcg ctgcgctcgg
     1501 cgggtgcaac caaagacgga ttcggcaaga tttcggcgtt catcatcgag acctcctcgt
     1561 aacgtgacaa tcttgcggaa gctgcggctc gacggcttga cccaaaccgc ctcgggtttg
     1621 accctatgcg ccaaactgcg tggtacttta cgggggcatg agtttcctcc ccgcggcctc
     1681 tatcctcgcg gttttggagg ggcttgcagc gctgaagctc gatgtcgcgc gcatacgtcg
     1741 cgaagtcttc cgagacgaag gcgagagcaa cccagaagcg ctcgtccctg cggcgttgtt
     1801 cggcgaggtg ctgcgcgcag cgcagatgca ggctccgcgc gaagagctct tgctcgaagc
     1861 ggcgctctcg gtcccactcg gcgcgttcgg cctcatggac tatctggccg cgagctcgga
     1921 cacgatcggc gaagcgttcg tctctttgag caggctgttt cccagcgtcg cgcccggctg
     1981 cgcgatcgag gtcgacgaaa gcccgcgcga atacgtcgtc acgctgcgaa acccgaacga
     2041 gcactcgctg cgcgtcaacg gtgacgaact cacggtcggc gtcatgctgc gccactttcg
     2101 gaacctgtc
//
LOCUS       DASTKS010000709         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1229811, whole genome shotgun sequence.
ACCESSION   DASTKS010000709 DASTKS010000000
VERSION     DASTKS010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229811"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1145
                     /locus_tag="VFQ35_14665"
     CDS             90..1145
                     /locus_tag="VFQ35_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931941.1"
                     /translation="MHERSRWAARRAKSPAAIVSGILFAAIVRPALASPAEAPSVELE
                     WVGPGTEAVCLGAAGLTRAVNDYLGRAAISDPPGERRVLVEVERRPGSWRARIELIDA
                     QSNASLGEREIIAEGALCSGLDEPLKLSVALLVDSDLTETPAAGAHEAPRPPTPTPEP
                     PAPTPTPPRDEPPPLFPARPWRFSWEASLLALNQVLPTVAVGAEVGLEAWASDSVVLR
                     AHGAAFAPQTHEVTADTAYKATVLYGGLGVCPGGELAPKWRLFGCFGFEGGGIWVTRT
                     GFNGGKDAARRLLAGSLGTRLTYDLGSKLSLGAQFSVLLPLQQERFVADADGKRQELF
                     GISPACWVVGLGVASAL"
     gene            1187..1720
                     /locus_tag="VFQ35_14670"
     CDS             1187..1720
                     /locus_tag="VFQ35_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9931942.1"
                     /translation="MNAGSARAVTPTPDLFAEIFRQHARYLWRALLGLGVRAADVDDV
                     CQEVLLVVHRRLPEFAGGAMRSWLFAICLRVASDYRRSARVRREISVAETPEVEHGRG
                     PLEDLQMRELWQRLLQGLDGLDEDKRAAFVLYEIEELTVREVAEAMGCPLQTAYSRLH
                     AARAHMKEHFGVEGREP"
     gene            1717..>2255
                     /locus_tag="VFQ35_14675"
     CDS             1717..>2255
                     /locus_tag="VFQ35_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931943.1"
                     /translation="MTQDPKRLIVSERNELGLGLASARRQLPNELELTRLARRLAEAG
                     VAVPADLVPGGTEARPPSPHERTRGGLRFKVYTGMGLGTLAVCAVLGARLQMRSPGSA
                     PHAAIRPANADVSSQATRILSAPAPFVPSPAPPAMPEAERSRETARALPPTAAEAATP
                     VVEASSKPLASTSRDAARRG"
BASE COUNT          340 a          786 c          758 g          371 t
ORIGIN      
        1 ccggatagct cggtcggcgc ggaggcccgc gtgtcaggca ctttttcgag gaggtcgctc
       61 gcgcaccgct gttataggct ctgccaacga tgcacgagag gtcccgttgg gctgcccggc
      121 gagcgaagag ccccgccgcg atcgtcagcg ggatcctctt cgccgccatc gtgcggcctg
      181 cgctcgcatc cccagccgag gctccctcgg tcgagctcga gtgggtgggc ccgggcaccg
      241 aagcggtgtg cctcggtgcg gccgggctca cgcgcgcggt caacgactac cttggacgcg
      301 cggccatttc ggacccgccc ggagaacgac gcgtgctggt cgaggtcgag cggcgacctg
      361 gctcgtggcg agcacgcatc gagctcatcg acgcgcaaag caacgcttcg ctcggcgagc
      421 gtgaaatcat cgcagaaggt gcgctgtgca gcggcctcga cgaaccgctc aagctgtccg
      481 tcgctttgct cgtcgacagc gatctcactg aaactcccgc tgccggcgcg cacgaggccc
      541 cccgccctcc gacgcctacc ccagaacctc cggcccccac gccaactcct ccccgggacg
      601 agccgccgcc gctcttcccg gcacggcctt ggcgcttctc gtgggaggct tcactactcg
      661 cgttgaacca agttttgcca accgtcgcgg tgggcgccga agtcgggctg gaagcttggg
      721 cgagcgactc ggtcgtgttg cgcgcacatg gggcggcgtt cgcaccgcaa actcacgagg
      781 taacggccga cacggcgtac aaagcgaccg tgctctatgg cgggctcggc gtgtgccccg
      841 gtggagaact cgcacccaag tggcggcttt tcggctgctt cgggttcgag ggaggtggaa
      901 tctgggtgac ccgcaccggc ttcaacgggg gcaaggacgc cgcccgccgg ttgctggccg
      961 gctccctggg gacgcggctc acctacgacc ttggctccaa gctctcgctc ggagcacaat
     1021 tctcggtgct cttaccgcta cagcaggagc gattcgtggc cgacgcagac ggtaaacggc
     1081 aggaattgtt cggcatttcg ccagcttgct gggtcgttgg gttgggcgtg gcgagcgcgc
     1141 tctgaccccg attcagaaat ccggcgagct gccgccatgg agtagcgtga atgccggttc
     1201 ggcacgcgcc gtaaccccaa cgcccgatct gttcgcggag atcttccgcc agcacgctcg
     1261 ctacctttgg cgagcgctcc tgggcctcgg cgtgcgtgcc gcggatgtgg acgacgtctg
     1321 ccaagaggtg ttgctggtgg tgcaccggcg cttgcccgag ttcgcggggg gcgccatgcg
     1381 aagctggctg tttgcgattt gccttcgcgt cgcgtccgac tatcggcgca gcgcgcgcgt
     1441 ccgccgcgaa atcagcgtcg ccgagacgcc tgaagtcgag cacggccgcg gccccctcga
     1501 ggacttgcaa atgcgcgaac tctggcagcg cctgctccaa ggcctcgacg gtctagacga
     1561 ggacaagcgc gccgccttcg tcctctacga aatcgaggag cttaccgtgc gtgaagtcgc
     1621 cgaggcgatg ggctgccctc tgcaaaccgc gtactctcgg ttgcacgcgg cgcgcgctca
     1681 catgaaagaa cacttcggcg tggaagggag agagccgtga ctcaggatcc gaagcggctg
     1741 atcgtgtcgg agcgcaacga gctcggtctc ggtcttgcga gcgcgcgacg tcagctgcca
     1801 aacgagctcg agctcacgcg gctcgctcgt cgcttggccg aggccggcgt tgcggtgccg
     1861 gctgacctgg ttccaggcgg caccgaagct cggccgccgt cgcctcacga aaggactcga
     1921 ggcgggttgc gcttcaaggt gtacacgggc atggggctcg ggacgctcgc ggtgtgcgcc
     1981 gtactcggcg cgcggctgca gatgcgctcg cccggaagcg ctccccacgc cgccattcgc
     2041 ccagcgaacg cggacgtgag cagccaagct acgcgcatcc tttccgcacc cgcacctttc
     2101 gtcccctccc ctgctccccc cgcaatgccc gaggcggaac gctcgcgcga gacggcgcgc
     2161 gcgctccctc cgaccgctgc cgaagccgcg acgcccgtcg tcgaagcctc gtcgaagccg
     2221 ctcgctagca cctcacgcga tgcggcgcgc cgcgg
//
LOCUS       DASTKS010000710         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_559793, whole genome shotgun sequence.
ACCESSION   DASTKS010000710 DASTKS010000000
VERSION     DASTKS010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559793"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..734
                     /locus_tag="VFQ35_14680"
     CDS             114..734
                     /locus_tag="VFQ35_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9931944.1"
                     /translation="MSITFYRAPMSSAIPVAWALSELSVPHEAVTFDLKAKEQKRPEF
                     LKLNPNGKVPTLVVDGVPMFEALAINIWLGERFGVEKGMWPAPGTLSAGEALSWSTWA
                     YVTYGSMATRVFLASGRWHGEGFENAAQLAVAKKEIAELHRILDERLAERAFILGPAF
                     TLVDLVVASAVGYASAGGISVADYPRLKAWLETCQARPACRAAWKT"
     gene            complement(757..1536)
                     /locus_tag="VFQ35_14685"
     CDS             complement(757..1536)
                     /locus_tag="VFQ35_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9931945.1"
                     /translation="MPLAIVTGANRGIGLQVSKDLVQLGYEVIVASRDARAGEQAAQE
                     ELGGEPRARSIQLDVSDASSVAAFVEALREGARVDALVNNAGTSFDGFDAEVARRTLD
                     TNYRGPVRLTRALLPRLSESANVVMVSSGMGELSSFGPALREKLLAPTLSADDVERTA
                     RDFEQAVAAGRQKALGFPSNAYAVSKALLNAFTRVLGKEFAGTRHRVNAVCPGWVRTR
                     MGGSSAPRSLEQGARGIVWAATLPDGGPNGQFFRDGKSIAW"
     gene            complement(1598..3343)
                     /locus_tag="VFQ35_14690"
     CDS             complement(1598..3343)
                     /locus_tag="VFQ35_14690"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET9931946.1"
                     /translation="MEPRKLTQVPPRKLKRTKNFDRRLWNRFWQIARPYWFGDEKWAA
                     RGLLALLILLLLGRTEFTVLFNQQTGEFTSALAAHDTTRFWRSIRFFGIVLVLAAPIY
                     AFYYYVRDRLGVAWRRWLTHGLLDRYLSHRAYYALGADASIDNPDQRIAADVATFTQK
                     SLGFLLDVVGAVLQLIAFSGVLWSISRALVIFLVVYAALGTWVTFGIFGKPMIGLNFQ
                     QLRREADLRFALIRVREHAEGIAFYRGEAREKVQVRERFKELYRNYLRLLRRTLGLNF
                     FQYAYSFVTLVVPSIIVAPRIISGELEVGRAVQASGAFAAMLSALTVFVENFETLSAF
                     AAGIERLHSFSKALATHGATNGHTVIAREPNDRLELDHLTVQTPNYQRTLLNEVSFAL
                     DPGHGLLIVGASGGGKSSLLRAIAGLWNSGSGTIRHPKLEESLFLPQRPYMILGTLRE
                     QLLYPQIDREVSEDELRRVLALVNLPDLEERCGGFDVDLDFGRVLSMGEQQRLAVARV
                     LLAKPRFVVLDEATSALDEQNEARLYRELKQNQITLISVAHRHAVLTHHDHVLEVTGD
                     GGWRVSAAKGYTFND"
     gene            complement(3467..>4846)
                     /gene="ligD"
                     /locus_tag="VFQ35_14695"
     CDS             complement(3467..>4846)
                     /gene="ligD"
                     /locus_tag="VFQ35_14695"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET9931947.1"
                     /translation="SKRAASLYAPGRGRDWVKTKCSQRQEFVVVGYTDPRGSRSGLGA
                     LLLAARDGKELRYSGRVGTGFSQKSLADLVKKLKPLALKKAPFEGAPHTGISDVHWVR
                     PKLVAEVAFTGFTSDGLLRHPRFEGLREDKSADEVVIERPEKSRRSKSDAGSSSKKRK
                     GGGEPTSGRAASFPLTNPDKVLFPEVGLTKRHILEYYELVAERMLPHVANRPLTLVRC
                     PNGWKGTCFFQKHPGQGLPDALRTVPIREKEGKAAYAVLDDVEGLFALVQLGALEIHT
                     WGSFADDFERPNILVFDLDPDPSVGFGAVMDCAKQLREIFSAAKLESFVKTTGGKGLH
                     VCVPIEPELEWPEIKAFCKELVEALVRQSPDRYVATVSKAQRRGKIFIDYLRNARGAT
                     FIAPYSTRARENAPVAVPLEWDELTSKFRPGAVTVSNIRTYLAEHRKDPFARLVTVTQ
                     RLPRAPLTK"
BASE COUNT          918 a         1548 c         1521 g          859 t
ORIGIN      
        1 cggcgtgaac gagcggctcg gcgcgtatcg aggaccgaaa ctgtccgcat tctagagtag
       61 gctccaaaga gttcgaaccg gcagctaacc cgtttaatta caaggagtta cccatgagca
      121 taaccttcta ccgagccccc atgtcgagcg ctatcccggt tgcttgggcc ctgtccgagc
      181 tctccgtccc gcacgaggcg gtgaccttcg acctcaaagc caaggagcaa aagcggcccg
      241 agtttctgaa gctgaacccg aatggcaaag tgccgacttt agtcgtcgac ggcgtgccga
      301 tgttcgaggc gctcgccatc aacatctggc tcggcgaacg gttcggcgtc gaaaagggta
      361 tgtggcctgc gccgggaacg ctttctgccg gcgaggcact gtcgtggagc acgtgggctt
      421 acgtgacgta cggcagcatg gccactcgcg tgttcctggc gtcggggcgc tggcacggcg
      481 agggctttga aaacgcggct cagctcgcgg ttgcgaagaa agaaatcgcg gagctccacc
      541 gaattctgga tgagcgccta gcggagcgcg ccttcatcct cggtcccgca ttcacgctcg
      601 ttgatctcgt cgtcgcgtcg gcggtcggct atgccagcgc gggtgggatc tcggtcgccg
      661 actatccgcg actgaaggcc tggctcgaaa cctgtcaggc acgccccgct tgtcgagcgg
      721 cgtggaagac ttgagaatgt tcgcctcggg ccgccgtcac caggcgatgc tcttgccgtc
      781 gcggaaaaac tgaccgttcg ggccgccatc aggcaaagtc gcggcccaaa cgatcccgcg
      841 cgcgccttgt tccaagctgc gaggtgccga gctaccaccc atgcgagtgc gcacccagcc
      901 cgggcacacg gcgttcacac ggtgccgtgt gccggcgaat tccttgccga gcacacgcgt
      961 gaacgcgttt aggagcgcct tcgagacggc gtaggcgttg ctcgggaagc cgagtgcctt
     1021 ctggcgcccg gcggccaccg cttgctcgaa gtcacgcgcg gttcgttcga cgtcgtccgc
     1081 agagagggtc ggcgcgagca gtttttcgcg cagcgccggg ccgaatgacg acagctcgcc
     1141 catgccgctc gataccatga cgacattggc gctctcgctg aggcgaggca gcagcgcgcg
     1201 cgtcagacga acaggtccgc ggtagttcgt gtcgagggtg cgccgagcga cctcggcgtc
     1261 gaagccatcg aacgaggtgc cggcgttgtt gacgagcgcg tcgacgcgcg cgccctcgcg
     1321 aagcgcttcg acgaaagccg caacgctcga agcgtcggag acatcgagct ggatgctgcg
     1381 cgctcgaggc tcaccgccga gctcttcttg ggctgcctgc tcccctgccc gtgcgtcgcg
     1441 agaggctacg atgacttcgt agccgagctg aaccaggtcc ttactgacct gaagaccgat
     1501 tccacgattt gctccggtca ctatcgcgag cggcatgagt ccctctcaat caggagacgc
     1561 gagcggccct gagcctgccg tctcatttac cggggtgtca atcgttgaag gtgtatcctt
     1621 tggccgcgct gactcgccag ccaccgtcgc cggtgacctc caacacgtgg tcgtggtgtg
     1681 tgagcactgc gtgacggtgc gctacgctaa tcagcgtgat ctggttctgc ttgagttctc
     1741 ggtagagccg ggcctcgttt tgctcgtcga gagcgctggt agcctcgtcg agcacgacga
     1801 aacgcggctt cgcgagtaac acccgagcga ccgccaggcg ttgctgctct cccatcgaca
     1861 gcacccggcc gaagtcgagg tcgacgtcga agcctccgca gcgctcttcg agatccggga
     1921 ggttcaccaa ggcgaggact cggcgaagct catcctcgga aacttcgcga tcgatctgcg
     1981 gatagagcag ctgttcccgc agggtgccca ggatcatgta gggccgctga ggcaagaaca
     2041 gcgactcttc gagctttggg tgccgtatcg taccggaacc agagttccag agcccggcga
     2101 tggcgcgcaa gagagagctc tttccaccac cgctcgctcc gacgatgagc aagccatggc
     2161 ccggatcgag cgcgaacgac acctcgttca gcagcgtccg ctggtagttc ggggtttgaa
     2221 cggtgaggtg gtcgagctcc aaacggtcat tcggctcgcg cgcgatcacc gtgtggccat
     2281 tcgtcgctcc gtgcgtcgca agcgccttcg agaaagagtg caagcgctcg atgccggccg
     2341 cgaaggcgct gagcgtctcg aagttttcga cgaagacggt caaggcgctg agcatcgccg
     2401 cgaaagcacc gctcgcttga actgcgcgac cgacttcgag ctcccccgaa ataatgcgcg
     2461 gcgcgacgat gatgctcggc acgacgagcg tcacgaacga gtaggcgtac tggaagaagt
     2521 tcaagccgag cgtccgccgc agcaaacgca ggtagttccg gtagagctcc ttgaagcgct
     2581 cgcgcacctg caccttttcg cgtgcctcac cgcggtagaa ggcgatgcct tcggcgtgtt
     2641 cgcgcacgcg aatcaatgca aagcgcaagt cggcctcacg ccgcaactgc tgaaaattga
     2701 gcccgatcat cggcttgccg aagatgccga acgtgaccca cgtcccgagc gccgcgtaga
     2761 cgaccagaaa aatcacgagc gcacgggaga tcgaccacag caccccgctg aatgcgatga
     2821 gctgcagcac cgcacccacc acgtcgagca aaaagcccaa gctcttctgc gtgaaggtcg
     2881 cgacgtcggc cgcgatacgt tggtcggggt tgtcgatgct cgcgtcagcc ccgagcgcgt
     2941 aataagctcg gtggctcagg tagcggtcca acaggccgtg cgtcaaccag cggcgccacg
     3001 cgacgcccaa gcgatcgcgc acgtagtagt agaatgcgta gatcggcgcg gcgaggacaa
     3061 ggacgatccc gaaaaagcgg atcgaccgcc aaaagcgagt ggtgtcatgc gccgcgagcg
     3121 cggaggtaaa ctcaccggtt tgctggttga acagcaccgt gaactcggtg cgccccagca
     3181 aaagtaggat gagcaaggcg aggagtccgc gggctgccca cttctcgtct ccaaaccaat
     3241 agggacgcgc tatctgccag aagcggttcc aaagccggcg gtcgaagttc ttggtgcgct
     3301 tgagcttacg gggcggcact tgagtgagtt tgcgaggctc caatcgctcg cattgtcaca
     3361 cgcagcaagc aatgtgccac gtcactcagg ggattattgc ctcggtttgc gccgagcgcg
     3421 taccaatgtg gcgcagcttc gccgccacat tcgcgccaag tcgccatcat ttcgtcaacg
     3481 gcgcgcgcgg caagcgctgc gttacggtga cgagccgcgc gaaaggatcc tttcggtgct
     3541 ccgcaagata agtgcggata ttgctcacgg tcacggcacc gggccggaac ttcgaggtga
     3601 gctcgtccca ttcgagcgga accgcgaccg gcgcgttctc acgggctcgc gtcgagtaag
     3661 gcgcgatgaa agtagcgccg cgcgcgttgc gcaaatagtc gatgaagatc ttcccgcgac
     3721 gctgcgcctt ggaaacagtc gcaacgtacc ggtcggggga ctgtcgcacc agcgcctcga
     3781 cgagctcctt gcagaaggcc ttgatttccg gccactcgag ctcgggctcg atcggtacgc
     3841 agacgtgaag ccctttgcca cccgtcgtct tgacgaagct ctcgagcttc gccgccgaga
     3901 agatctcgcg cagctgcttg gcgcaatcca tgaccgctcc gaacccgacg gacggatcgg
     3961 ggtcgaggtc gaataccaga atgttcggcc gctcgaagtc gtctgcgaac gagccccagg
     4021 tgtgaatctc gagcgcgccc agctgaacca acgcgaagag gccttcgacg tcgtcgagca
     4081 ctgcgtaggc cgccttgccc tctttctcgc gaatgggaac cgtgcgcaac gcatccggaa
     4141 ggccttggcc cggatgcttt tggaaaaagc aggtgccctt ccaaccgttg ggacagcgca
     4201 cgagggtgag cggtcggttg gccacgtgcg gcaacatgcg ttccgcgacg agctcgtaat
     4261 actcgaggat gtggcgcttc gtgaggccca cctccgggaa cagcaccttg tccgggttcg
     4321 tcagtggaaa cgaagccgcg cgccccgaag tcggctcgcc accacccttc cgcttcttcg
     4381 atgagcttcc cgcgtccgac ttcgaacgac gtgacttctc gggccgctcg atcacgacct
     4441 cgtccgcact tttgtcttcg cgaagtccct cgaaccgagg atggcgcaac aagccgtcac
     4501 tcgtgaaccc ggtgaaggcg acctccgcca cgagcttcgg gcgcacccaa tgcacgtcac
     4561 tgatgcccgt gtgcggcgcg ccctcgaacg gtgccttctt cagcgcgagc ggcttcagct
     4621 tctttaccaa gtccgccagc gacttttgac tgaaccctgt ccccacccgc cccgaatagc
     4681 ggagctcttt cccatcgcgc gccgccagta gcaacgcacc gagacccgag cgtgaacctc
     4741 ggggatcggt gtacccaacc acgacgaact cttggcgctg ggagcacttg gttttcaccc
     4801 agtcgcgacc acggcccggt gcgtagagcg aggcggcgcg cttcga
//
LOCUS       DASTKS010000711         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1900368, whole genome shotgun sequence.
ACCESSION   DASTKS010000711 DASTKS010000000
VERSION     DASTKS010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900368"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFQ35_14700"
     CDS             complement(<1..1248)
                     /locus_tag="VFQ35_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931948.1"
                     /translation="MSQTSAPPSRPNNAAPSPALPSPALPSPAGAPSSSPKSSPVSSP
                     ISVAPSSRKTMPPLSRKSAPPGEAQQRPLDFRIIRRLLGFTGSYSRIRNLLAILVVAR
                     AVQLPLVTWGTARVISGPIARRDLVGSALGVVALIAFAGFTELCFVYRSRLALQLGEG
                     VVHDLRNLIYAHLLRQPMSFFKRTQVGRLIGRVTSDVDVVRTGVQDVAFVTVVQLGNL
                     LVSAALMAYYDFKLFLVVLTMAPILWYIVGYFRKRFSLAYRAQQESFSRVTATLAEAV
                     NGIREIQGFVRQNVNGGLFGQLVYDHSRYNMGAARQGAVFGPLLEFNGQLFLAILLVA
                     GGYQALVHEVRLEALIQFFFLSNALFAAIPNIGNQYNQALTAMAGAERVFSLLDTEPE
                     WEDREDAQEIPSIKGKVELKELCF"
     gene            complement(1245..>2265)
                     /locus_tag="VFQ35_14705"
     CDS             complement(1245..>2265)
                     /locus_tag="VFQ35_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9931949.1"
                     /translation="VNHLNVGVLLLYGGYLVVKSRLTIGDLVVFAGLLQQFSAQISSM
                     AGIVNTLQQSLAGARRVFEVLDAKLEVESPPAPRRIDRFEGRIRFEQVSFGYEESDEV
                     LRDIDFEVTPGRCVAILGLTGSGKSTLLSLIPRFYDTTKGRVLIDGVDVRDLELDTLR
                     RNIGLVFQESLLFRNTVAQNIAFGHPDASREAVENAARIAGAHEFICELTNGYDTVLE
                     EGAVNLSGGQRQRIAIARALLLQPRILLLDDPTTAIDPETEREVLSAMDSAMQGRTTL
                     VVANRLSTLRRADEILVLSDGRIVERGTHDQLMAEHGVYFRAASLQAADNESLMLLGA
                     LEAQA"
BASE COUNT          422 a          737 c          728 g          378 t
ORIGIN      
        1 gaagcacagc tccttgagct caaccttgcc tttgatgctc gggatctcct gcgcgtcttc
       61 gcggtcctcc cactctggct ccgtgtcgag cagggagaac actcgctccg ccccggccat
      121 cgccgtgagc gcttggttgt attgattacc gatgttgggg atggccgcga acagcgcgtt
      181 cgagaggaag aagaactgaa tcaaagcctc gagccgcacc tcgtgcacga gcgcctggta
      241 gccgcccgca accagcaaga tcgccaagaa gagctgtccg ttgaactcca agagcggacc
      301 gaaaacggcg ccctgtcgcg ccgcgcccat gttgtaacga gagtggtcgt agacgagctg
      361 gccgaagagg ccgccattta cgttctggcg aacgaagcct tggatctcgc ggataccgtt
      421 caccgcctcg gccaaggtgg cggtgacgcg gctgaagctc tcttgttgcg cgcggtaagc
      481 gaggctgaac cgcttccgaa aataacccac gatgtaccaa aggatcggcg ccatcgtgag
      541 cacgaccaag aacagcttga agtcgtagta cgccatcagc gcggcggaga ccaacagatt
      601 accgagctgc acgacggtga cgaacgccac gtcctgcacg cccgtccgca ccacgtcgac
      661 gtcggaggtg acccgcccga tgagccgccc cacctgcgtg cgcttgaaga agctcatcgg
      721 ctgacgcaag aggtgcgcgt aaattaggtt tcgcagatcg tgcacgacgc cttcgccgag
      781 ctgcagggcg aggcgcgagc ggtaaacgaa gcaaagctcg gtgaagcccg cgaacgcgat
      841 cagggcgacg acgccaagcg cgctacccac cagatcgcga cgcgcaatcg gcccgctgat
      901 cacgcgcgcg gttccccagg tgacgagcgg caactgaacc gctcgcgcga cgacgaggat
      961 cgcgagcaga ttgcggatcc gcgaataact gccggtgaaa ccgagcaagc ggcggatgat
     1021 cctgaaatcg agggggcgct gttgagcttc tcccggtggc gcgctcttgc gactgagcgg
     1081 cggcatcgtc ttccggctgc tcggcgcgac gctgatcggg ctgctcaccg gcgagctctt
     1141 cgggctgctc gacggggctc cagcggggct cggcagcgcg gggctcggca gtgcggggct
     1201 cggcgcagcg ttgttggggc gactcggcgg ggcgctcgtt tggctcatgc ttgcgcctcc
     1261 aaggctccga ggagcatgag gctctcgttg tcggccgcct gcaacgacgc cgcgcggaag
     1321 tacacgccgt gctcggccat cagctggtcg tgggtgcccc gctcgacgat gcgaccatcg
     1381 ctcaacacca ggatttcgtc cgcgcgccgc agagtcgaca gccggttcgc gacgacgagc
     1441 gtggtccggc cttgcatggc gctatccatt gctgaaagca cctcgcgctc ggtctcggga
     1501 tcgatcgcgg tggtcgggtc gtcgagcagc aagatgcgcg gctgcaacag aagcgcacgg
     1561 gcgatggcga tgcgctgcct ctgcccaccc gacaggttca ccgcgccttc ttccagcact
     1621 gtgtcgtaac cgttcgtcag ctcgcagatg aactcgtgcg cgcctgcgat gcgagccgca
     1681 ttctccaccg cctcgcggct cgcgtccgga tgtccgaagg cgatgttttg cgccaccgta
     1741 ttgcggaaca agaggctctc ctgaaacacg agaccgatgt tcctccgcaa cgtgtcgagt
     1801 tcgagatcgc gcacgtcgac gccgtcgatg agcacgcgcc ccttggtcgt atcgtagaag
     1861 cgcggaatca agctgagcag ggtgctcttt ccagagccgg tcagccccag gatcgcgacg
     1921 cagcgcccgg gcgtgacttc gaagtcgatg tcgcgcagta cctcgtcgct ctcttcgtag
     1981 ccgaacgaga cctgctcgaa gcgaattcgc ccttcgaagc ggtcgatgcg gcgcggcgcc
     2041 ggggggctct cgacctcgag cttcgcgtcg agcacctcga acacacgccg ggccccggcg
     2101 aggctctgct gcagcgtatt cacgatgccc gccatgctgg agatctgcgc cgagaactgc
     2161 tggaggagcc ccgcgaacac cacgagatca ccaatggtca ggcgcgactt caccaccaga
     2221 taaccgccgt acagcaagag cacgccgacg ttcaggtgat tcacg
//
LOCUS       DASTKS010000712         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1230343, whole genome shotgun sequence.
ACCESSION   DASTKS010000712 DASTKS010000000
VERSION     DASTKS010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230343"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFQ35_14710"
     CDS             complement(<1..208)
                     /locus_tag="VFQ35_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931950.1"
                     /translation="MNPSPVKSALCFLAMASLAACGASPVARTTASEATSRAGPSATE
                     ATQLELTYLGVAGWQLRSSEHVLLI"
     gene            complement(205..546)
                     /locus_tag="VFQ35_14715"
     CDS             complement(205..546)
                     /locus_tag="VFQ35_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931951.1"
                     /translation="MTSFCIRFSVGLLVGTALLHFALAARMGSTNALLLAAPGLVLAG
                     MTWLMRIRWVLWVGLSVLVLLETCALVGYASSDASTRSVIVWAFAIALSSVQLPIGAL
                     VAAARYFESAE"
     gene            complement(603..1517)
                     /locus_tag="VFQ35_14720"
     CDS             complement(603..1517)
                     /locus_tag="VFQ35_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9931952.1"
                     /translation="MADDSPFHRGELLVQERVGSRERVDRAGRRMIRDYMPDEHRTFF
                     EELPFLVVGSIDDAGRPWASILSGEPGFVTSPHPRSLMVAALPNRDDPLARNLRAEAP
                     LGVLGIQLETRRRNRANGKVQWCSERGFELFVEQSFGNCKQYIHTREPRAIAAHSTAE
                     HGSHRLSPRALELIRGCDTAFIASVSEHPRQGSREGVDVSHRGGSPGFIRVEPNGATT
                     RLTLPDYRGNFLFNTLGNIEVNPRAGLVCCDFETGDLLTLTGTAKVIWQGAEIATFDG
                     AERLLELEVEEYVLLGGGLGQTWAKTAA"
     gene            complement(1520..2149)
                     /locus_tag="VFQ35_14725"
     CDS             complement(1520..2149)
                     /locus_tag="VFQ35_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET9931953.1"
                     /translation="MTTPNAPLTLYYFPLSGHSHRAQLLLSLARLPHELVNVDLASGA
                     HKRPEFLKKNPFGLVPVLEHGDFVIAESNAILLYLAETFASAAPYWPSDARSRAGVQR
                     WLSVACGPLQRGPCLARVIRVFGRQFDHAEAVAVAQSLFGVMEQELAGRAFLVGGAPT
                     LADLAMYTYTAHAPEGDVSLEPYPNIRSWVARIQGLPHFVPMPSAPRRG"
     gene            2229..3155
                     /locus_tag="VFQ35_14730"
     CDS             2229..3155
                     /locus_tag="VFQ35_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9931954.1"
                     /translation="MDRLECMQMFVAVAETGGFASAARRVGVSPPVVTRAVANLEEHL
                     GARVLTRTTRKVRLTEAGARYFEDCRRLLAELDDVEAAVSGEYAELRGALNITASVMF
                     GRLYVSSIVLDFLAQHRDVTARVFLSDRVLDLIDEGLNLAVRIARLADSSLTALKVGE
                     VRRVVCASPAYLKKHGIPKTPEELADFDAIAFAPERSAPSWVFEGKRGKVSVKPRAQL
                     MANASEVGIQAALAGRGLTRVLSYMVASELRAGRLRLVLEDYEPEPLPIHIVYREGQR
                     APARVRAFADFASERLRKDLSMNLRPRRTGSD"
     gene            3335..>3815
                     /locus_tag="VFQ35_14735"
     CDS             3335..>3815
                     /locus_tag="VFQ35_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9931955.1"
                     /translation="MEAETQRPKILVVDDTAENRALAQATLEDEDYLVIQASSGEEAL
                     ALFERERPDCVLLDVRMPGMDGLTVCQRIRAMESGAETPVVFLTALRDVDTFDQALRA
                     GGDDFLTKPVRPTELLVRVQAALKLRRMSTELREHYDLVRHQRDDLMRLQLQKERLTA
                     "
BASE COUNT          694 a         1204 c         1305 g          612 t
ORIGIN      
        1 cgatgaggag gacgtgctcg gagcttcgaa gctgccatcc ggcgacgcct aggtaggtga
       61 gctcgagttg ggtcgcttcg gtcgcgcttg gccctgccct cgaggtggct tcgctcgccg
      121 tcgttcgcgc aacaggggaa gcgccgcacg cagcgagcga agccatcgca agaaagcaga
      181 gcgccgactt cacgggcgaa ggattcactc cgcgctctcg aagtagcgag cggcggcaac
      241 gagcgcgccg atcggcagct gcacgctgct caacgcgatc gcgaacgccc acacgatgac
      301 gctcctcgtg gaggcgtccg acgaagcgta gccgaccaag gcgcacgtct cgagcagcac
      361 caagaccgaa agcccgaccc ataggaccca ccgaattcgc atgagccacg tcatacccgc
      421 gagcaccagg ccaggagccg ccaacagcag cgcgttcgta gagcccatcc gcgcggcgag
      481 cgcgaaatga agcaacgcgg tgccgacgag aagccccacc gagaagcgga tgcagaagga
      541 ggtcacggcc gcgttctagc gcaagcggag cgcaccggac accgaagcct cgacgagccg
      601 cttcacgctg cggttttcgc ccaagtctgg ccgaggccgc cgcccaagag gacgtactcc
      661 tccacctcga gctcgaggag ccgctcggcc ccgtcgaagg tggcgatctc ggcgccctgc
      721 cagatgacct tcgccgtgcc ggtgagagtg agtagatctc ccgtttcgaa gtcgcagcac
      781 acgaggccgg cgcgcgggtt gacctcgatg ttgccgaggg tgttgaagag gaagttgcct
      841 cggtagtcgg gtaaagtgag tcgagtcgtc gcgccgttcg gctcgacgcg gatgaaccct
      901 ggcgagccgc cgcggtgtga aacgtcgacg ccttctcgac tgccttgccg tgggtgctcc
      961 gacaccgacg cgatgaacgc ggtgtcgcag cctcgaatca gctcgagcgc tcgaggtgag
     1021 aggcgatggg agccgtgctc agccgtcgaa tgcgctgcga tcgcgcgcgg ttctctcgtg
     1081 tggatgtact gtttgcagtt gccgaacgac tgctccacga agagctcgaa gccgcgttcg
     1141 ctgcaccatt ggaccttgcc gttcgcgcga ttgcgacgtc gcgtctcgag ctgaatcccg
     1201 agcaccccga gcggcgcctc ggcgcgcaag tttcgagcga gcggatcatc gcggtttggc
     1261 agcgccgcca ccatcaacga gcgcggatgc ggcgacgtga cgaagccggg ttcgccggac
     1321 aagatcgaag cccaggggcg gcccgcgtca tcgatgctac ccaccacgag aaacggaagc
     1381 tcctcgaaga aggtgcgatg ctcgtccggc atgtagtccc ggatcatgcg gcgaccggcg
     1441 cggtcgacac gctcgcgcga gccgacgcgc tcttggacga gaagctcgcc gcggtggaaa
     1501 ggggagtcgt cggccatggt tagccgcgcc ggggcgcgct cggcatcggg acgaagtggg
     1561 gcaacccttg gatgcgagca acccagcttc gaatgttggg atacggctcg agcgaaacgt
     1621 cgccctccgg cgcgtgcgcc gtgtacgtgt acatcgcgag gtcggcgaga gtcggtgcgc
     1681 cccccaccaa aaaggctcgt cccgcgagct cctgttccat cacgccgaac aggctttgcg
     1741 caacggctac ggcttcggcg tgatcgaact gacgcccgaa gacgcgaatc actcgcgcca
     1801 gacacggccc gcgctgtagc ggcccgcaag caaccgacag ccaacgctga acaccggcgc
     1861 ggctgcgcgc gtcgctcggc cagtacggcg ccgccgacgc aaacgtctcc gccaagtaga
     1921 gcagaatggc gttgctctcc gcgatgacga agtcgccgtg ttcgagcacc ggcacgagcc
     1981 cgaacgggtt cttcttcaag aactccggcc gcttgtgggc gcccgaagcg aggtcgacgt
     2041 tcacgagctc gtgcggcaag cgagcgaggg agagcaagag ctgcgcccga tgggaatggc
     2101 cggagagagg gaagtagtag agcgtcaacg gcgcgttggg tgtcgtcatg cagggaggat
     2161 gcggcaccgc gcgtacagaa acaatccgtc gcgttgacaa tgaactattt ccaaaacagg
     2221 aattatgtat ggatcgtctc gagtgcatgc aaatgttcgt ggccgtcgcc gaaacgggag
     2281 gttttgcgag cgcggcgcgg cgggtcggcg tgtcgccgcc ggtcgttacc cgcgccgtcg
     2341 cgaacctcga ggagcatctc ggcgcgcgtg tgctgacccg gacgacgcga aaggtgcggc
     2401 tcaccgaggc tggggctcgc tacttcgaag actgccggcg cctgctcgcc gagctcgacg
     2461 acgtcgaggc ggccgtgtcg ggggagtacg ccgagctgcg cggcgcgctg aacatcaccg
     2521 cgtcggtgat gttcggcagg ctttacgtgt catccatcgt tctggacttc ttggcgcaac
     2581 atcgcgacgt caccgcgcgc gttttcttga gcgaccgcgt gctcgatctc atcgacgaag
     2641 ggttgaacct cgcggtgcgc atcgctcggc tcgcggattc gtcgctcacg gcgctcaagg
     2701 tcggtgaggt gcgccgggtg gtctgcgcct cgcctgccta cttgaagaag cacggcattc
     2761 cgaagacgcc ggaggagctc gccgacttcg acgcgatcgc gttcgcaccg gagcggtcgg
     2821 cgccgtcatg ggtcttcgag ggcaagcgcg gcaaagtgag cgtcaagcct cgcgcccagc
     2881 tcatggcgaa cgcttcggag gtcggaatcc aagcagccct ggccggcaga gggctcacac
     2941 gcgtgctctc atacatggtc gcgagcgagc tacgcgcggg ccgactgcgg ctcgtgctcg
     3001 aagactacga gccggagccg ctccccattc acatcgtcta tcgagagggc cagcgcgctc
     3061 cggctcgggt ccgcgctttc gccgatttcg cgtctgagcg tctgcgcaag gatttgtcga
     3121 tgaatctccg gccccggcgc acgggctcgg actgaggttt gctgccgcgc tgccacagat
     3181 agcacgtaca tcaagcgtag atattcggcc ggcgcgttgg taccgggcgg ggaggtgact
     3241 ttggacggca aaagtgcggc agatcagggc atacagcgtc acgggtgcgc tgcaggtccc
     3301 atagggctat gctggcgcga aaccccaatc ggcgatggaa gcagagacgc agcgacccaa
     3361 aatcctggtc gtcgacgaca ccgcggagaa ccgcgcgttg gcgcaggcga cgctcgagga
     3421 cgaagactac ctggtcatcc aagcgtcttc gggggaagag gcgttggcgt tgttcgaacg
     3481 cgagcgcccc gactgcgttc tgctcgacgt tcgcatgccg ggcatggatg ggctgacggt
     3541 gtgtcagcgg atccgcgcga tggagagcgg ggccgagacg ccggtcgtct tccttacggc
     3601 tttgcgcgat gtcgacacct tcgatcaggc gctgagggcg ggcggcgacg acttcttgac
     3661 gaagccggtt cgcccgacgg agctcctggt gcgcgttcag gcggctttga agcttcgacg
     3721 catgagcacc gagctgcgtg agcactacga cttggtgcgt caccagcgtg acgacctgat
     3781 gcggctccag ctccagaagg agcgcctcac cgcct
//
LOCUS       DASTKS010000713         9674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1005679, whole genome shotgun sequence.
ACCESSION   DASTKS010000713 DASTKS010000000
VERSION     DASTKS010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005679"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFQ35_14740"
     CDS             <1..514
                     /locus_tag="VFQ35_14740"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9931956.1"
                     /translation="GTFFEGETVLLMAGYAAHAGRLSFGWVVVAAFFGSLVGDQTAFW
                     FGHHYGTRLLSRFPRLRPQVERASALLRRVQAPLLLGFRFVYGIRVATPIAAALAKVP
                     LVRFVVLNALGAAVWAVVITGAGYLFGHGISRVFEHAKHFEHIVLGVIAALGVALTLV
                     HWLRRRTVTE"
     gene            complement(504..1925)
                     /locus_tag="VFQ35_14745"
     CDS             complement(504..1925)
                     /locus_tag="VFQ35_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931957.1"
                     /translation="MQAARWSVLEALVNLALWVTFATIKTRAFAGKAPLYTGDKRVWA
                     TIAEAPFFSREFLLPRRPIVYPLLFKAGLSDPAIVSLQLWLSIVSWGLFAYALSRFVP
                     KLIGPIVFAATLFFALVTPVNAWDLIIRSESTSHSLVVLTMASALLFCRALTQGRRRA
                     IALAALTVLVGTASVFARDTNPFIVLLLLPTLGMWVWSVRRESRVTRRTLLTLTISVA
                     VALSAASYTAQWTARAAPRFDFPLMNVIFTRVLPNPVKRDYFVRELGMPMSHALMSRK
                     GRFASANGRRAYVDPALHTFRLWVFIEGYSAYQRYLLTHLEEASREALSEFPRVAGYS
                     PGREGVHSATSVTPLADEWLVLGVLARSPLEACGVFFGIGLLAAILRWTKTRVLGALL
                     VFLVLATLSQLFLCVHGDSMELYRHAVSVGILLRFALLVGVAIGASWLVSLVGVSAAW
                     GRRRLAARRNAVEPPKAPGPATP"
     gene            complement(2143..3231)
                     /gene="modC"
                     /locus_tag="VFQ35_14750"
     CDS             complement(2143..3231)
                     /gene="modC"
                     /locus_tag="VFQ35_14750"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HET9931958.1"
                     /translation="MSLEPSVRAELRLARENLSIEMRLELPARGVSVFFGRSGAGKTT
                     FLRLIAGLERGRGTLVACGECWQDDARKVFVPTERRGIGYVFQEASLFSHLTVRGNLN
                     YALERRSNSRAAPSEASILEILDIGQLLSRAPSSLSGGERQRVAIARALLSAPRLLLM
                     DEPLASLDDSRKAEVLPYLERLRDELSIPIVYVSHSIDEVARLADWLVLVENGRAERE
                     GTLTELLSRLDSPLSHGEQAGVVLDARITQHDPEHHLSHVEFPGGGLWVTEVKKPLGS
                     VVRARVLARDVSIALSEPGPSSILNVMPARVSDISDEGPDRVNVALQVGSGGTKLLAR
                     VTRRSLRALDLSPGAPVYAQVKSVALVT"
     gene            complement(3221..3898)
                     /gene="modB"
                     /locus_tag="VFQ35_14755"
     CDS             complement(3221..3898)
                     /gene="modB"
                     /locus_tag="VFQ35_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET9931959.1"
                     /translation="MTLSAGDASAIRVTLELASVTTVVLLLIGTPISWWLAFGSSRWK
                     GAVATCVALPLVLPPTVIGFYLLLLLGPNGFVGRITNAVGVGNLPFTFPGLVLASTVY
                     SLPFVVQPLQNAFEGVGRRPLEAEATLGAGPLDRIRTVAFPLARRGYLTATIVGFAHT
                     MGEFGVVLMIGGNIPGKTRVLSVAIYDHVEALEYDQAHTLAAVMLAFSFVVLFALYTL
                     RAKPSES"
     gene            complement(3895..4689)
                     /gene="modA"
                     /locus_tag="VFQ35_14760"
     CDS             complement(3895..4689)
                     /gene="modA"
                     /locus_tag="VFQ35_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9931960.1"
                     /translation="MTSKNRATLTVTTLFALALVLLVPARRAHAEEARVAVASNFVGP
                     MRKLSAEFTKRTGHTTVVVSGATGKLYAQIKNGAPFDMLLAADEKTPRQLEADGLAAP
                     NESFTYAVGKLVLFSPKTNFVKDGPALLRAGSFEHLAIANPKLAPYGSAAVAVMTKLG
                     VFEALSPKLVQGENIAQTLQFVESGSAEVGFVAWSQILEGGKPRAGSYWLVPESLYPP
                     ILQDAVVLRRASKNRAANAFAAFLKTDVALRVISEAGYATPSKAKE"
     gene            complement(4686..5558)
                     /locus_tag="VFQ35_14765"
     CDS             complement(4686..5558)
                     /locus_tag="VFQ35_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9931961.1"
                     /translation="MALLTTKQAAKLLNVHPKQLYRLLKQGLPAARVGTEWRFESDAV
                     LRWARRDGTEVSQTANEPELVRAAADSYRAAPLLAANGDLVIDELLAALHASGGPLLG
                     FVLADQAAAQTYLARNTVLLGGNHEDAPLPSSPFKRVRLHLATREIGIATRASSPLRK
                     LSELTRRSVALRPGSAGVRASLDAALTRASISLEDAYRRASEYASHREVALAVASGIA
                     DAGLTTRAWAVAARLAFHPIASEAYGLCVRADSLADPCVVRVCELSQTASFRNRLRDA
                     YGYGVERTGELKFS"
     gene            complement(5977..6360)
                     /locus_tag="VFQ35_14770"
     CDS             complement(5977..6360)
                     /locus_tag="VFQ35_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9931962.1"
                     /translation="MSDEGTSVSSTTAKLPDVVSATPEEFDALLEARRDALLVLYMWG
                     HDCPNCEFFAKRLPELLPQLAGAPLVLVKVNVYERPEFARRYGVFGIPHFLLFSQGRR
                     LGKMSEFRGDSFWLNVIREHLPQVA"
     gene            complement(6374..7258)
                     /locus_tag="VFQ35_14775"
     CDS             complement(6374..7258)
                     /locus_tag="VFQ35_14775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9931963.1"
                     /translation="MSVPALRDVSDTARWVAYFRAVESERPDALFADPYARVLAGERG
                     QRIAEQLPKGPLSWSIAVRTKVFDELVLEAITSHGARTVVNLAAGLDTRPYRLPLPPD
                     LRWVEVDLPELVASKTDALRRERPRCRVERMALDLADADARRALFSQFDHEPGCVLVV
                     TEGLLVYLEEEVVTALARELREYFPTGLWLMENISPAVLARMKRLWESTLYKAAATMK
                     FAPPEGLRFYEPLGWAPVAERALIEEAGRFRREMRVVTVIRALGRILPPVGAAFARRQ
                     ERFRDAVVYALMAPKPPQ"
     gene            complement(7255..7545)
                     /locus_tag="VFQ35_14780"
     CDS             complement(7255..7545)
                     /locus_tag="VFQ35_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931964.1"
                     /translation="MANRHTHKKLRAEVRARMTATGESHQTALAHILARGQADPAVPD
                     LIAVNYFGVTINVAVFEVLAHLRVVLVSGSGVPLGLPLGSALPLRVHTDGVQ"
     gene            complement(7742..>9674)
                     /locus_tag="VFQ35_14785"
     CDS             complement(7742..>9674)
                     /locus_tag="VFQ35_14785"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9931965.1"
                     /translation="PAADDMGHDVIGSLKLSNGKGFQARGLTHALTLTRALTLTRALV
                     MGLGVWCSGCGSSEEPIAPKCSPGQFAAADSAALFGAPAVARIDVALRGSTWKQLMSK
                     ARDEEYAAVDACYNGESIGTVGLRFKGQAGLSRCFDGDELICKKLSMRLKFDYADHEL
                     RFFGLKHLNLHSLTGDASHVRERLAYDLYRDMSVDAPRSDWAELVVDGKSQGLYSMVE
                     QIDGRFVASRWPDASDGDLYKETWPTSTDPEYYREGLETHEETASPTALVEFASSLAQ
                     APPEAGLSVISQFMDANQLFRYMAVDDATANWDGVTALYAKEDGTFRNHNFFIFEKPD
                     GAPGPRLQVIPWDMDHTFELPNWRLGSPPWRDTASACPQVVLGCLVADCDPILHALGS
                     NANAYRDAVHELLQGPFAEGLIESQLSRHAARIEAAVGRDALGPSVSEWRAEVDGVRA
                     SLPVLRQYMQRRADGPALERLTIPAEGSADFSRYDELELLFGISVLAAPTARASVTKS
                     PADSLVGGIRLNFALPRESQGWAVYFLPLATPNLDVRSKTGVRFRVRNAGANYVGLNI
                     DSAVGDVGNTRWTWPLELDEREKSYELHFSDLVWPDPSVTGPAKDLVLAHVQELVLVV
                     ANQGQASQGFVDVADFELF"
BASE COUNT         1796 a         3092 c         3147 g         1639 t
ORIGIN      
        1 ggggaccttc ttcgaaggcg agacggttct gctcatggcc ggttacgccg cccacgctgg
       61 tcgactgtcc ttcgggtggg tggtcgtggc ggcgttcttc gggagcctgg tcggagatca
      121 gaccgcgttc tggttcggtc atcattacgg gacgcggctc ctcagtcggt tcccgcgcct
      181 gcgtccccag gtcgagcgcg cttcggcgtt gctgcgtcgc gtgcaagcac cgctgctcct
      241 cggctttcgc ttcgtgtatg gcattcgcgt tgccacgccg atcgccgctg ctctcgccaa
      301 agtgcctttg gttcgattcg tcgtcttgaa cgcgctgggc gcggctgtgt gggcggtcgt
      361 aatcaccggc gctggttatc ttttcggtca cggcatcagc cgagtcttcg agcacgcgaa
      421 gcacttcgaa catatcgtgc tgggcgtcat cgcggcgctg ggcgtcgctt tgaccctcgt
      481 gcactggctg cgacggcgaa cggttacgga gtagccggtc cgggcgcttt aggcggctcg
      541 acggcgttgc gtcgcgctgc gagcctacgg cgaccccacg ccgcgctcac gccgacgagg
      601 gaaacgagcc agctcgcgcc gatggcaacg ccgaccagca gcgcgaaccg tagcaagatg
      661 ccgacgctca cggcgtggcg gtacagctcc atcgagtcgc cgtgaacaca caagaagagc
      721 tgagacaagg tggcgagcac caagaagacg agcaacgcgc ccagcactcg cgtcttcgtc
      781 caacgtagga ttgccgcgag gagaccgatg ccgaagaaga ccccgcacgc ttcgaggggt
      841 gagcgagcca gaacgccgag cacgagccac tcgtcggcga ggggcgtcac ggaagtcgct
      901 gaatgcacac cttctcggcc ggggctgtaa ccggcgacgc gcggaaactc gctcagggcc
      961 tctctggacg cttcttcgag gtgagtgagc aggtaccgct gataagccga atatccctcg
     1021 atgaacaccc agaggcgaaa cgtgtggagc gcgggatcga cgtaggcgcg gcgaccgttg
     1081 gcggacgcga acctcccttt gcggctcatg agcgcgtgcg acatgggcat gccgagctca
     1141 cgcacgaagt agtcgcgctt caccgggttc ggcaagaccc gcgtgaagat gacgttcatc
     1201 agcgggaagt cgaagcgcgg cgcggcgcgc gcggtccact gcgccgtgta cgacgcggca
     1261 ctgagcgcca ccgccaccga aatcgtcagt gtgaggagcg tgcgccgagt gactcgtgac
     1321 tcgcgcctca ccgaccaaac ccacattccg agcgtcggca agagcaggag aacgatgaac
     1381 ggattcgtat cgcgcgcgaa gacactcgcg gtgccgacga gcaccgtgag cgcggccaac
     1441 gcgatggcgc gccgacgccc ttgggtcaac gcgcggcaaa acaacaacgc cgaggccatc
     1501 gtcagcacga cgagcgagtg cgacgtcgac tccgagcgga tgatcaggtc ccaggcgttc
     1561 accggcgtga cgagcgcaaa gaagagcgtc gccgcgaata cgatgggccc gatgagcttg
     1621 gggacgaagc gcgagagcgc gtacgcgaag agcccccagc tcacgatcga gagccacagc
     1681 tgcaggctga cgattgcggg atcgctcaac cccgccttga acaacagcgg gtaaacgatg
     1741 ggacgtcggg gtagcaagaa ctcgcggctg aagaatggcg cctcggcgat cgtggcccac
     1801 acgcgtttgt cgcccgtata gagcggcgct ttcccagcga acgctcgggt cttgatcgtc
     1861 gcgaaggtga cccagagcgc gaggttcacc agcgcttcga gcaccgacca gcgcgccgcc
     1921 tgcacgcgtg cccagtcaag gtgagcgaac gcgtgtcggc gcgcataccc ccacaaatcg
     1981 aagctgggac gccgagcagt caaactgacg ggcgttttag ttgcacgcct cccaagtcac
     2041 aatcgttttg aagggcggcg gccgagtgtg gctccgcagt tggcccgaag tcccagcggc
     2101 tcgcctgtcg tgacggggcg ctggcgcttt ggtagcgtgc ggtcaggtga cgagcgccac
     2161 gctttttact tgggcgtaaa cgggagcgcc gggtgagagg tccagtgccc ggagggagcg
     2221 tcgcgtcacg cgagccaaga gcttcgtgcc accggaccca acctgcaaag cgacgttcac
     2281 ccgatcgggt ccctcgtcgc tgatatccga gacccgcgcc ggcatgacgt tcaggatgga
     2341 ggatggcccc ggttcactga gggcgatgct gacgtccctc gccaacacgc gcgctcgaac
     2401 gacgctgccg agcggctttt tgacttcggt gacccaaagc ccgccaccag ggaactcgac
     2461 atggctgagg tgatgctcgg ggtcgtgctg cgtgatacgc gcgtcgagca cgacgccggc
     2521 ctgttcgccg tggctgagtg gtgagtcgag acgactcagg agttcggtga gcgttccctc
     2581 ccgctcggcg cgcccgtttt cgaccaagac cagccagtct gcgaggcgcg cgacttcgtc
     2641 gatcgaatgg ctcacgtaca cgatcgggat cgagagctca tctcgcaggc gctcgaggta
     2701 cggcagtact tcggctttgc gactgtcgtc gagcgaagcg agcggttcgt ccatcagcag
     2761 gagacgcggc gcggacagga gcgcgcgcgc gatggcgacg cgctggcgct cgccgcccga
     2821 aagagagctc ggggcgcgcg agagtagctg accgatgtcg aggatctcga ggatgctcgc
     2881 ttcgctgggt gcggcgcgcg agttcgagcg acgctcgagc gcatagttca agttgccgcg
     2941 gacggtcagg tgcgaaaaca ggctcgcttc ctggaatacg tagccgatgc cgcggcgctc
     3001 ggtcggaacg aatactttgc gagcatcgtc ctgccagcac tcgccgcacg caaccaaagt
     3061 tccccgtccg cgctcgagcc ccgcgatgag ccgaaggaag gtggtcttgc cagcgccgga
     3121 tctcccgaaa aaaacgctca cgccgcgcgc gggtaactcg agccgcattt cgatcgaaag
     3181 gttctcgcgg gcgagccgaa gctccgcgcg taccgagggc tcaagactca cttggttttg
     3241 ctcgcaaggt gtacagcgcg aagagcacga cgaaagagaa ggccagcatc accgccgcaa
     3301 gcgtgtgcgc ctgatcgtac tcgagcgctt cgacgtgatc gtagatggcg accgacaaca
     3361 cgcgcgtctt cccagggatg ttcccgccga tcatcaatac gactccgaac tcgcccatgg
     3421 tatgcgcgaa gccgacgatg gtcgcggtca ggtagcctcg acgcgcgagc gggaacgcga
     3481 cggtgcgaat acggtcgagg ggccccgcgc ctagcgtcgc ctctgcctcc agcggacgcc
     3541 gacccactcc ttcgaaagcg ttttgcagag gctgcaccac gaaaggcagc gaatacaccg
     3601 tcgacgcgag caccagcccc ggaaacgtga acggaaggtt gccgacaccg acggcattgg
     3661 tgattcgccc cacgaacccg ttcggtccca gcaggagcag gagataaaag ccaatcacgg
     3721 tgggcggcaa tacgagcggc agcgcaacgc acgttgcaac cgcgcccttc cacctcgagg
     3781 agccgaacgc caaccaccac gagatgggtg ttccaatcag caggagcacc accgtggtaa
     3841 cgctcgcgag ctcgagcgtc acccgaatcg cggaagcatc gccggcgctc agcgtcattc
     3901 cttggccttg ctcggcgtcg cgtagccagc ctccgagatc acgcgaagtg cgacgtccgt
     3961 cttcaggaac gcggcgaaag cgttggcggc gcgatttttc gaagctctgc gcagcacgac
     4021 cgcgtcctgc aggatcggtg ggtagaggct ctcgggcacc aaccaatacg agcccgcgcg
     4081 cggcttaccg ccctccaaga tttgcgacca agcgacgaat ccgacttcgg cgctgccgct
     4141 ctcgacgaat tgcagcgttt gcgcgatgtt ttcgccttga acgagcttcg gcgaaagagc
     4201 ctcgaagacg ccgagcttcg tcatgaccgc gaccgccgcc gagccatagg gagcaagctt
     4261 tgggttcgcg atcgcgaggt gttcgaacga accagcgcgc agcagcgctg gaccgtcctt
     4321 cacgaagttc gttttggggc tgaacaagac gagcttgccg acggcgtaag tgaagctttc
     4381 gttgggggcc gcgagcccat ccgcctcgag ctgacgcggc gtcttttcgt ctgcggcgag
     4441 gagcatatcg aaaggagcgc cattcttgat ctgtgcataa agcttgcccg tagcgcccga
     4501 cacgacgacc gtcgtgtggc ccgtgcgctt cgtgaactcc gccgaaagct tgcgcatcgg
     4561 accaacgaag ttggacgcta ctgcgacgcg agcttcctcg gcgtgggcgc ggcgcgctgg
     4621 aacgaggagg acgagcgcga gggcgaagag tgtggtgacg gtaagcgttg ctcgattctt
     4681 ggaagtcatg agaatttcag ctctccagtg cgttcgacgc cgtacccata ggcgtcgcgc
     4741 aacctatttc ggaaggaagc ggtctggctg agctcgcaga cccgcacgac acacggatcg
     4801 gcgagggaat cagcgcgtac gcacaagccg tacgcctccg aagcgatggg gtggaaggcg
     4861 aggcgcgccg caaccgccca ggctcgcgtc gtcagacccg cgtctgcgat gccactcgcg
     4921 acggcgagcg cgacttcacg atgcgaggcg tactcgcttg ctcgacggta ggcatcctcg
     4981 agtgagatgg aagcgcgcgt cagcgccgcg tcgaggcttg cccgaacccc agcgctcccg
     5041 gggcgtaacg ccacgctgcg gcgagtcagc tcggacagtt tgcgcaacgg cgacgaggcg
     5101 cgcgtagcga ttccgatttc gcgcgtcgcg aggtggaggc ggacccgctt gaacggtgag
     5161 ctcggcaagg gcgcgtcttc gtgattgcct ccgagcagta cggtattccg cgccaggtac
     5221 gtttgggcgg cggcctggtc cgcgagtacg aagccgagca gcggcccgcc gctcgcatgg
     5281 agagcagcca gcagctcgtc gatcacgaga tcgccgttcg ccgcgagcag cggggcggct
     5341 cggtacgagt cggctgccgc tcgcacgagc tcgggctcat tcgcggtttg tgaaacctcc
     5401 gttccgtccc gtcgggccca ccgcagcacc gcgtcgctct cgaaccgcca ttcggtcccg
     5461 acgcgcgcag ccggcagccc ctgcttcagc agccggtaga gctgcttggg gtgaacgttg
     5521 aggagcttcg ctgcctgctt ggtggtgaga agcgccatcg aggacgaatg ttacccatcg
     5581 ctcctcacca gaccgcaagc agcggcgata tcatgggtta tttcaatggg atagcttgtg
     5641 tttcaggcgt gttcccattg atcctcgctc gtccaggtca ggggttcttg tcgccgctct
     5701 cgggtgcgga tccgggttga tcgttcctgc gggaactacc ggcgcgatcg cggctttggg
     5761 ttggcgcctt gtgcggcgac gactgcgact gctggggtgc ggggcgttgg ttcgactgag
     5821 acttttggga ccggtccgga tgtttgtcgg agttctgttt ggtgttcatt ggagtgcctc
     5881 ttcccttccg ggtttcgaga tgcactccat gtgccggcga agcgcgctct acgacttcgc
     5941 cccaacaccg caatcggctt acgtccgctc gagcgtttac gcgacctggg gcaggtgctc
     6001 gcggatcacg ttcagccaaa acgagtcgcc gcgaaattcg ctcattttgc cgagcctgcg
     6061 accctgtgag aagagcagaa aatgcgggat cccgaagacg ccgtaacggc gcgcaaactc
     6121 cgggcgctcg tacacgttca ccttgaccag gacgagcggc gcgcctgcga gttgtggcaa
     6181 gagctcgggg aggcgcttcg cgaaaaactc acaattgggg cagtcatgcc cccacatgta
     6241 caagacgagc agcgcgtcgc gccgagcctc gagcagcgcg tcgaactcct ctggtgtggc
     6301 agagactacg tcgggaagtt tcgcggtcgt gcttgagacc gaagtccctt cgtcggacat
     6361 tcgtcagcat agctcattgc ggtggcttcg gtgccatcag cgcgtagacg accgcgtcgc
     6421 gaaaccgctc ttgccggcga gcgaaggcag cgccgaccgg cggcagaatt cgcccgagcg
     6481 ctcgaatgac ggttacgacg cgcatttcgc gacggaagcg ccccgcctct tcgatgagcg
     6541 cgcgctcagc caccggcgcc cagccgagcg gctcgtagaa ccgcaggccc tccggcggcg
     6601 cgaacttcat cgttgcggcg gccttgtaga gggtgctctc ccaaagccgc ttcatgcgcg
     6661 cgagcacggc gggcgaaatg ttctccatca accacaagcc ggtcgggaag tactcgcgca
     6721 gctcacgagc gagcgcggtg accacttctt cttcgaggta cacgaggagg ccctcggtga
     6781 cgacgagaac gcatccgggt tcgtgatcga attgggagaa gagcgctcgt cgcgcatctg
     6841 cgtccgcgag atcgagcgcc atgcgctcca cgcgacaacg cggccgctct cgacggagag
     6901 catccgtttt cgatgccacg agctcgggca agtcgacttc gacccagcgc aagtccggcg
     6961 gtagtggaag gcggtacggt cgggtgtcga gtcccgccgc gaggttcacc accgtgcgcg
     7021 ctccgtgcga cgtgatcgct tcgagaacga gctcgtcgaa tactttggtc cgaacggcga
     7081 tggaccacga caagggccct ttcggcagct gttcggcgat acgctgtccg cgctctccag
     7141 caaggacgcg cgcataagga tctgcgaaga gcgcatcggg acgctccgac tcgacggcgc
     7201 gaaagtaggc gacccagcgc gccgtatcgg aaacgtcgcg cagcgcgggg acactcattg
     7261 gacaccgtcc gtgtgcactc gcagcggcaa agcgctcccg agcggcaaac cgagggggac
     7321 gcccgaaccc gagaccaaaa cgactcgcaa gtgcgccagg acctcgaaga cggctacgtt
     7381 aatcgttacg ccaaagtagt tcaccgcgat gaggtcggga acggccgggt ccgcttgccc
     7441 tctcgcaagg atgtgagcca gcgcggtttg gtgactttcg ccggtcgcag tcatacgcgc
     7501 gcgcacctcg gcgcgaagct ttttgtgggt atgtcggttg gccatgctca agctttctcg
     7561 gtgagacctg cccggcacag gagaccgatt tcgagcggca aacgactaca cccgaagggc
     7621 cgcgccctct tcggcgccga ttgagtcttg ccggggacga ctcgtcaacg ccagggctcg
     7681 ggaagagccc ggaagcaacg atagtgcagc gcccgcttct gtcaacgaca acctcggtcg
     7741 atcagaatag ctcgaaatcg gcgacgtcga cgaagccctg cgacgcctgt ccttggttag
     7801 ccaccaccaa gacgagctct tgcacgtgtg cgagtaccag gtccttcgct ggccccgtga
     7861 cgctcggatc cggccagacc aaatcggaga agtgaagctc gtacgacttc tctctttcgt
     7921 cgagctccag cggccacgtc cagcgcgtgt tgcccacgtc gccgaccgcg ctatcgatgt
     7981 tcaaccctac gtaatttgcg cccgcattgc gcacccgaaa tcgcacgccg gtcttgctcc
     8041 tgacgtcgag gttcggagta gcgagtggca agaagtagac ggcccaaccc tgcgactcgc
     8101 gcgggagcgc gaaattcagc cgaataccac cgacgagcga atcggctggg ctcttcgtca
     8161 cggaagctcg cgcggtcggc gcagccaaaa ccgagatacc gaagagcaac tcgagttcgt
     8221 cgtagcgcga gaagtccgcg ctgccctccg cggggatggt caggcgttcg agcgcgggcc
     8281 cgtcggcgcg gcgctgcatg tattgtcgca acactggcaa tgacgcccgc acaccgtcga
     8341 cttcggcgcg ccactccgag actgaggggc ccagcgcgtc gcggccgacc gcagcttcga
     8401 ttcgcgccgc gtgccgcgag agctgcgact cgataaggcc ctccgcgaat ggcccttgca
     8461 gtagctcgtg taccgcgtcg cgatatgcgt tcgcgttact cccgagcgca tgcaagatcg
     8521 gatcgcagtc cgctaccaag catccgagaa cgacctgtgg acatgcggac gccgtgtcgc
     8581 gccacggcgg cgagccgagc cgccaattcg gtagctcgaa ggtatgatcc atgtcccacg
     8641 ggatgacctg cagccgcggc ccaggagctc cgtccggctt ctcgaaaatg aagaagttgt
     8701 gattccgaaa tgtgccgtcc tctttggcgt agagcgcggt gacgccgtcc cagttcgccg
     8761 tcgcgtcatc gaccgccatg taacggaaga gctgattcgc atccatgaat tgcgaaatga
     8821 cgctcagccc ggcctccggc ggcgcttgcg caaggctact cgcgaactcg acgagcgcgg
     8881 tgggcgacgc cgtctcttcg tgggtttcga ggccctcacg atagtactcg ggatccgtcg
     8941 acgtcggcca tgtctccttg tagaggtcgc cgtcgctcgc gtcgggccag cgcgaagcca
     9001 cgaagcgccc atcgatctgc tcgaccatcg agtaaagccc ctgcgacttc ccatcgacga
     9061 cgagctccgc ccagtcgctc cgaggggcat cgacgctcat gtctcgatag agatcgtacg
     9121 cgagccgttc gcgcacgtga gaggcgtctc ccgtcagcga atgcaagttc aggtgcttca
     9181 acccaaagaa gcgaagctcg tggtccgcgt aatcgaactt cagccgcatg ctgagcttct
     9241 tgcaaatcag ctcgtcgccg tcgaagcagc gagacaggcc cgcttgccct ttgaaccgaa
     9301 gtccgaccgt cccgatcgat tcgccgttgt aacaggcgtc gacggcggcg tactcttcgt
     9361 cgcgcgcctt cgacatcaac tgcttccagg tggacccacg gagcgccacg tcgatgcgcg
     9421 cgaccgccgg cgcgccgaac aacgcggccg aatccgccgc ggcaaattgg cccggagagc
     9481 actttggcgc aatcggctct tcactgctgc cgcacccgga gcaccacacg ccgagcccca
     9541 tgacgagcgc gcgggtgagc gtgagcgcgc gggtgagcgt gagcgcgtgc gtcaaacccc
     9601 ttgcctggaa ccccttgccg ttgctgagct tcagcgaccc aatcacgtca tgccccatgt
     9661 catcggctgc tgga
//
LOCUS       DASTKS010000714         5572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_114665, whole genome shotgun sequence.
ACCESSION   DASTKS010000714 DASTKS010000000
VERSION     DASTKS010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114665"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1278)
                     /locus_tag="VFQ35_14790"
     CDS             complement(214..1278)
                     /locus_tag="VFQ35_14790"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9931966.1"
                     /translation="MCTVDSKARPWQMAANDMSADSLSVRGVAPPLDERRRRFARYAV
                     WLLGFTLLVVLFGAVVRITGSGAGCGKHWPTCQGEIVHLPKRVETLIELSHRLSTGLN
                     ALLVFALTFRATRIFDRKHPARRAFKFASAMMVVESLVGAALVLLALVGTDASMRRAI
                     VMPLHLVATSGLCAALGLGVFYSLPPAEAPPAALSVRRGVWLAAAAVLVVSASGALTA
                     LGDTVYPVQSAPLAARLLHDQGSGAHFLERLRVIHPLLAIAGAVVVLSSSARILGDRV
                     SEFARTLARAAICACLAQVGLGGLNIWLSAPGWMQVVHLLAANVVWLVLVLLAAELGQ
                     PRAVSLGVGRPRKLANLRAS"
     gene            1435..2745
                     /gene="xylA"
                     /locus_tag="VFQ35_14795"
     CDS             1435..2745
                     /gene="xylA"
                     /locus_tag="VFQ35_14795"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HET9931967.1"
                     /translation="MSFFPGIERIRYEGPESKNPLAFRYYDPDRIVLGKKMTDHLRVA
                     VCYWHTFAWPGNDIFGAGTFDRPWFKPGNPLELAELKLRTAFEFFEKLGSPFFTFHDR
                     DIAPEGATLRESHDNLDRIIEKAAAEMQRTNVKLLWGTANLFSNPRFMSGAATNPDPE
                     VFAFAAAQVRKILDITNQLNGANYVLWGGREGYETLLNTDMKREADQLARFLSLVAEH
                     KHKIGFKGLLLIEPKPKEPTKHQYDYDTASVYAFLQRYKLEKEYRVNIEANHAILSGH
                     SFQHEVAYALANGMFGSIDMNRGDDLLGWDTDQFPNDIQEIALVMHTIVKGGGFTTGG
                     MNFDAKLRRQSIDPEDLFHAHIGGMDTLARGLLIAEKMIQDGALDKIVEDRYAGWRGD
                     LGEKILGGRVSLADLSDLVLQKGIEPKPRSGRQEAIENLVNRYI"
     gene            complement(3118..3972)
                     /locus_tag="VFQ35_14800"
     CDS             complement(3118..3972)
                     /locus_tag="VFQ35_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9931968.1"
                     /translation="MRGPSARPAIVCWGETVWDEIAGREPSLGGVACSVATHLQLLGA
                     SPRLVSAVGADAHGEGAFRELEQRAVDTRWLLVVADGQTARVRVELDSSGPRYSALAR
                     LDWSLVHFDAALAQAVVAADALVFALFMQGTTVPLAALDRALASHHRPRLVGCDLNLR
                     HTTSRETIEKALTLVDFVKLNDVELRRLIDLYRTPDAASLLLQRKSNLAFVVETRGSQ
                     GARLIQRGRTTEVPAPLSAPPRHSVGAGDALMAAFVYALVSGDPPERALEEAVRYASQ
                     HVALNVRR"
     gene            4049..4432
                     /locus_tag="VFQ35_14805"
     CDS             4049..4432
                     /locus_tag="VFQ35_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931969.1"
                     /translation="MLVPDPFDIIAILLGIFLALRKSDVRAEDPARYPQVAIADFDRW
                     RARAISAYSLGIRACFTKVLADYLFLFFLQRFSMVLWLQRAIGVSITVIWVGLLIACW
                     IGARRARRLAGELRIEQVRDAAPSA"
     gene            complement(4507..>5572)
                     /gene="lepB"
                     /locus_tag="VFQ35_14810"
     CDS             complement(4507..>5572)
                     /gene="lepB"
                     /locus_tag="VFQ35_14810"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9931970.1"
                     /translation="GLRTDLPREMRHDFEDGMHLLEEADRLMHRNRASIERQLSADAR
                     EELQEALEGLRDQIDRKEFLPGEFMAAYERASDLVARRLSRWRKGELREYIESIGFAM
                     GVALLLRAFVVEAFKIPSGSMLPTLQIQDHIFVNKLSYGPMIPFLHYRLFSSLPPKRG
                     DIMVFEYPDPNPANERQDFIKRAIAFEGDTLEVESGHPIINGWRVPSCRVGAYEFNEG
                     SGYGMKHGELYIEFLGDYSYLTLFEDTAFTERRQGPYHVKPGETWVMGDNRNNSSDSR
                     AWNGGRGGGVPDANIKGRAMFVWLSFGTAAAVTWDRLFTNVLGKPRLPKEAPAELVQG
                     IERCLAQRPAQTLPPPPKTH"
BASE COUNT         1052 a         1780 c         1777 g          963 t
ORIGIN      
        1 ccgtgaggcc cgagcagcac gaaagcgaga cgatgagcag cctggcaatg tcgctcgatc
       61 agcgcctctc gcgcccaagg ctcgccgctg cgagcagcga ggaccaggtc cgcgtcgttc
      121 agggacggcg cgaccctgcg aatgctccga atgaccttca atggctctct tgttcatcag
      181 agcgacgagc cgggtgagtc gggtcaaaag agtctagctg gccctcaaat tcgcgagttt
      241 tcgggggcgg ccaaccccga gggataccgc tcgcggctgt ccaagctcgg cggcgagcag
      301 caccagcacg agccatacga cgttcgccgc gagtaggtga accacttgca tccagcccgg
      361 agcggagagc caaatgttca atccgccaag gcccacttgg gcgaggcagg cgcagatcgc
      421 agcgcgcgcg agcgttcgcg caaattcgct cactcgatcc ccaaggattc gagcagaact
      481 actcagaacg acgaccgcac ctgcgatggc gaggagcggg tgaatgacgc gcagtcgctc
      541 gaggaagtgc gcgccgctgc cctgatcatg cagcaggcga gctgcgagcg gcgccgactg
      601 gaccggatat accgtatcgc cgagcgcggt cagcgcgccg gatgccgaca ctacgagaac
      661 ggcggccgct gcgagccaca cgccgcgtcg caccgaaagc gctgccggcg gggcttcggc
      721 tggcggcaac gaatagaaga ctccgagccc gagcgcggcg cacagccccg atgtggccac
      781 caggtgaagc ggcatgacga tggcgcggcg catcgacgcg tcggtcccca ccagggccag
      841 caaaaccagc gcggcgccaa cgagcgactc gacgaccatc atcgcgctcg cgaacttgaa
      901 ggcacgccgc gccgggtgct ttcggtcgaa gattcgagtg gcccggaaag taagcgcgaa
      961 aaccagaagg gcgttgagcc cggtgctgag gcgatgtgaa agctcaatca aggtctcgac
     1021 gcgcttcggc aaatgcacga tctcgccctg acaggttggc cagtgcttgc cgcagccggc
     1081 gccggatccg gtgatgcgta cgacggcccc gaacagcacg acgagaagtg tgaacccgag
     1141 caaccaaacc gcgtagcgag cgaaccttct tcgccgctcg tcgagcggcg gcgcgacgcc
     1201 gcgaaccgag aggctgtccg cactcatgtc gtttgccgcc atctgccatg gacgcgcttt
     1261 gctgtcgacc gtgcacatga cgcatcctgg aactgagcga aatcgtagcc gatgcggttt
     1321 gcctgggctt ttaaactaag ttaagttata cgcccgctgc gagttcggac acgtgccgac
     1381 gcggttttca gtgaaccaag ccacagcgct gcacccaaag gaatcacggt catcatgagt
     1441 ttcttccctg gcatcgagcg cattcgctac gaaggccccg agagcaagaa tccgctggcg
     1501 ttccgctatt acgatccgga ccggatcgtt ctcggtaaga agatgacgga tcatcttcgc
     1561 gtcgccgttt gctactggca caccttcgcg tggccgggga acgacatctt cggcgcgggc
     1621 accttcgatc gtccgtggtt caagcccggc aacccgctcg agctcgccga gctgaagctc
     1681 cgcaccgcgt tcgagttctt cgagaagctc ggctcgcctt tcttcacgtt tcatgaccgc
     1741 gatatcgctc cggaaggcgc gacgctgcgc gagagccacg acaacctcga tcgcatcatc
     1801 gagaaggcgg ccgcggaaat gcagcgcacc aacgtgaagc tgctctgggg cacggccaac
     1861 ctcttctcca acccccgatt catgagcggc gcggccacga accccgatcc cgaggtgttc
     1921 gcgttcgccg ccgcgcaggt gcgcaagatc ctcgacatca cgaaccaact caacggagca
     1981 aactacgtgc tctggggtgg ccgtgagggc tacgagacgc tcctcaacac cgacatgaag
     2041 cgtgaggccg atcagctcgc gcgcttcctc agcttggttg cggaacacaa gcacaagatc
     2101 ggtttcaagg gcctgctgct catcgagccg aagccgaagg agccgacgaa gcaccaatac
     2161 gattacgaca cggcttccgt gtatgcgttc ctccagcgct acaagctcga gaaggaatat
     2221 cgcgtcaaca tcgaggcgaa ccacgccatc ctctccggcc acagcttcca gcacgaggtc
     2281 gcgtacgctc tcgccaacgg catgttcggc agcatcgaca tgaaccgcgg cgacgacctg
     2341 ctcggttggg acacggacca gttcccgaac gacatccaag aaatcgcgct cgtcatgcac
     2401 accatcgtca aaggcggtgg gttcacgacc ggcggcatga atttcgatgc caagctccgt
     2461 cgccagagca tcgacccgga ggatctgttc cacgctcaca tcggcggtat ggacacactc
     2521 gcgcgcggtc tcctgatcgc cgagaagatg atccaggacg gcgccttgga caagatcgtc
     2581 gaggaccgtt acgccggttg gcgcggtgac ctgggcgaga agatcctggg cggccgcgtc
     2641 tcgctcgccg acctttcgga tctcgtgctc cagaagggaa tcgagccgaa gccgcgttcg
     2701 ggtcgccaag aggcgatcga gaacctcgta aaccgctaca tctgagcaca gctcagcgag
     2761 cgggtcgcaa cggccgcgtg gtctcgtcgt caggcggggc gcgcggccgt tgcgcttttc
     2821 ggcaaccaca gaatgaatcg aggacaacct gtagcgagca gctcgagcgc gcgagaacgc
     2881 agcccacaaa aaggcaacgg ctcgaagcct tggggaggga tgcttcgagc cattgagtag
     2941 ttaacgcggc ggccgtcctc cgggggaggg gagcaccaga ggcgccgcca gcgaatgccg
     3001 atattgcagc cgctgtgcca gctgattctc tcggtttttc ccgcgaaagt ttggcggttg
     3061 tcagcgtgct tgcgtcagtt cgggacaatc gcgacaaccc gcgttccccg ctgagaatca
     3121 tcgccgcacg ttcaaggcga catgctggct tgcgtagcga accgcttcct cgagggcgcg
     3181 ctctggggga tcgccgctca ccaacgcata cacgaaggcg gccatcaagg cgtcgcccgc
     3241 tccgactgaa tgccgaggag gtgcgctgag cggagccggg acctcggtag tgcgccctcg
     3301 ctgaatcagg cgtgctccct gcgagccccg cgtctcgacg acgaaggcga ggttgctctt
     3361 ccgttgaagg aggagcgagg cggcgtcggg agtgcgataa aggtcgatca gacgccgtag
     3421 ctcgacgtcg ttcagcttca cgaaatctac gagcgtgagc gccttttcaa tcgtctctcg
     3481 actcgtcgtg tggcgcaggt tgagatcgca gccgacgagg cgcgggcgat ggtgcgatgc
     3541 gagagcgcga tcgagcgcgg cgagagggac ggtcgtcccc tgcatgaaca atgcgaacac
     3601 gagtgcatcc gcggcgacga ctgcctgcgc gagcgccgcg tcgaagtgga cgaggctcca
     3661 gtctaagcgc gcgagcgccg aataccgagg tcccgacgaa tcgagctcga cgcggacccg
     3721 cgctgtttgg ccgtcggcga cgaccaggag ccaccgcgta tcgactgcgc gttgctcgag
     3781 ctccctgaat gcaccttcgc cgtgagcgtc tgctcccacg gcgctcacca ggcgcggcga
     3841 tgcgccgagc agctgcaagt gggtcgcaac ggaacaggca acgcctccga gggacggttc
     3901 tctgcccgca atttcgtccc agacagtctc tccccagcac acgatcgccg gccgagccga
     3961 cggcccgcgc atgagcgagg cgccgtttcg tgctaaggtg cgcacctcgc cgctcggccc
     4021 cgcgggcgcg tcgctctctg gacccagaat gctcgtaccg gatccgttcg atatcatcgc
     4081 gatcctacta gggattttcc tcgcgttgcg caagagcgac gtgcgcgcgg aggacccggc
     4141 gcggtatccc caggtagcga tcgcggactt cgatcgctgg cgagcgcggg ctatcagtgc
     4201 gtattccctc ggcattcgtg cgtgtttcac gaaggtcctt gccgactact tgttcctgtt
     4261 tttcctgcag cgcttctcga tggttctctg gctgcagcgc gcgatcggcg tttcaatcac
     4321 ggtgatttgg gtggggctgc tcatcgcatg ctggatcggt gctcgtcgcg cgcgccggtt
     4381 ggcgggggag ctccggatcg aacaggtgag ggacgccgct ccgtccgctt gagtgcgttc
     4441 ccggcgcgcg cgtggcgccg cgccgtgggt catccaagcg agcgcacggc gtcgcgccgg
     4501 cgcttctcag tgagtctttg ggggaggcgg tagggtttgc gcggggcgct gagcgaggca
     4561 gcgctcgatg ccctgtacga gctccgcagg agcttctttc ggtaggcgcg gcttgccgag
     4621 cacgttcgtg aacaggcgat cccaggtgac cgccgcagcg gttccgaagc tcagccacac
     4681 gaacatcgcg cgccctttga tattcgcgtc gggaacgccg ccgcctcgcc cgccattcca
     4741 ggcgcgggag tcgctcgagt tgttccggtt gtcacccatc acccacgtct cgcccggctt
     4801 cacgtgatac gggccctggc ggcgctcggt gaacgcagtg tcttcgaaga gcgttaggta
     4861 cgagtagtcg ccaaggaact cgatgtagag ctccccgtgc ttcatgccgt agccgctgcc
     4921 ctcgttgaac tcgtaggcgc caacacggca gctcggcact cgccaaccgt tgatgatcgg
     4981 gtgacccgac tcgacctcca acgtgtctcc ctcgaaggcg atggctcgct tgatgaagtc
     5041 ctgacgctcg ttggctggat tcgggtcggg gtattcgaag accatgatgt cgccgcgctt
     5101 cggaggcagc gaagagaaca agcgataatg cagaaacggg atcatgggcc cgtaactcaa
     5161 cttgttcacg aagatgtgat cttgaatctg cagcgtcggc aacatcgagc cgctcgggat
     5221 cttgaacgcc tcgacgacga aggcgcgcag caagagcgcg acgcccatcg cgaaaccgat
     5281 ggactcgatg tattcgcgca gctcgccctt acgccatcgg ctcagccgcc gggcgacgag
     5341 atccgatgct ctctcgtatg cggccatgaa ctcgccgggc agaaactctt tgcgatcgat
     5401 ttggtcgcgc agcccctcga gcgcttcttg cagctcttcg cgcgcgtccg ccgagagctg
     5461 ccgctcgatc gatgcacggt ttctgtgcat caagcgatcc gcttcttcga gtaggtgcat
     5521 gccgtcctcg aagtcgtggc gcatttcgcg gggtaggtcg gtgcgaagac cg
//
LOCUS       DASTKS010000715         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1901255, whole genome shotgun sequence.
ACCESSION   DASTKS010000715 DASTKS010000000
VERSION     DASTKS010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901255"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..2632)
                     /locus_tag="VFQ35_14815"
     CDS             complement(86..2632)
                     /locus_tag="VFQ35_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9931971.1"
                     /translation="MTTNNAASSRTAPLPDLTRSQAEATVSSLRQTAADTSALAESLT
                     RAATSGSQRAAASQQTHAALQAAAASIEDTLEMLMKLTRGQADFDAATRALSQSTEEG
                     SRLLVRVSREAEAITKDAENAASSSESMAATIEESARSIRSVAANSDETASATEQLLA
                     TITEMAAGLVSLAGGSESNAGNVAEVGATAEQMAKGIERLASDARDMRQRMASAMESM
                     HALRASGAEVSDQAQALASAVAQTASTAEQMARSVAGVATHARTLEAASMENAAGLAE
                     IAASVEQVAVSSERTSRAIDSNATTMEQMAKSSSSVTRSAEEIRRLSESSATAASQLE
                     KSTQRIAKVTEGAQVASERAATLARDGGATVGKSIHGLSKIRDSLTSSAGVMREMTRR
                     ADEIGEFVQTINLIADRTNLLSLNASIEAARAGEHGRGFAVVADEIRALADRAATASS
                     DIAKVIRSLQNTARDALTTTVEGERVANEGGALAAEAQAALSSIVGSIGELGGSVREV
                     FAASSEQVDAARSLSSVANQVRERTREIAQAIQEQAKAHDELSRTAAEVRQMAKQSTQ
                     ASMEQARALRDVVKANDQLAASAKQVAHATSEQAASASDLSRVAAQMRTTTQATAGSI
                     TQQTSLINAVAAEIQEGSVAVERTSTALAEQSKGASEIARAMDVARKDVALAARGIAE
                     QTRAGKELETAARQVSRLAAEVTRATSEQGKAMASLGKAADESRRLAAQTARAVNEQR
                     ELVGSVNDAFVAQASCLKETSAAAAQQAHTTQQIARTVEDARARVRESAANAAEQTRG
                     MDLLADELRDLVVRVTRLRDSGFGNGVALQSVLDALNSPSSGPTAEPREP"
     gene            complement(2553..3062)
                     /locus_tag="VFQ35_14820"
     CDS             complement(2553..3062)
                     /locus_tag="VFQ35_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9931972.1"
                     /translation="MTSLYVGFVVAGTEYALAASDVLELEAFSGATEVPSTLPHVEGI
                     VAVRGKVMPVLDLRTLFGQERVAPTRDTRVLVTRRGARVVALRVDSAREVLKLEPSQF
                     QSAPALAAHEAAAYVSSVCMLGQRLLLILALSRLLGEEDEHHDDEQRRLIPNSSATGP
                     HALPSRGDR"
     gene            complement(3059..>3717)
                     /locus_tag="VFQ35_14825"
     CDS             complement(3059..>3717)
                     /locus_tag="VFQ35_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9931973.1"
                     /translation="PAPQSASELLDLLCVAGFSTRQVANTTSGRGMGMEIVKRIVVGE
                     LGGELELETAWGAGTKFTLRVPLTLSIVDAFLFECRAQRFVVPVSTVEEIVHVDPDEI
                     VYGPRANDRGPAVGVIARRGRSIPIVMLRELFNWGAAEASPKAIVVKRAGAALAFGVD
                     RMLGQQEVVVRPIEDPLIRLPGVTGATDLGDGKPTLVLDLIAFSEVLSAGRHRAESPS
                     "
BASE COUNT          510 a         1281 c         1209 g          717 t
ORIGIN      
        1 agcaaagcgt ccacgtcgag gctgccgtcg ggcgaacctc gttcgagcgt cgcgatgcgc
       61 tcgagatcgg gagaggaggc gccgctcatg gctctcgagg ctccgcggtg gggccgctcg
      121 acggcgaatt cagggcgtcc aatacgctct gcaacgccac tccgtttccg aacccagaat
      181 cgcgcagccg agtcacacgc acgaccaaat ctcgcagctc gtctgcgagt aggtccatgc
      241 cgcgcgtttg ctccgccgcg ttcgcggccg actctcgcac ccgtgcgcgc gcgtcctcca
      301 ccgtgcgagc gatttgttgg gtggtgtgag cctgttgcgc cgccgcggcg ctggtctcct
      361 tcaggcagct cgcctgcgcg acgaaagcgt cgttcaccga gcccacgagc tcgcgctgct
      421 cgttcaccgc gcgcgcggtt tgtgcggcga ggcggcgaga ctcatccgcg gccttgccga
      481 gagaggccat ggctttgccc tgctccgacg tcgcgcgggt cacctcggcc gccagtcgcg
      541 acacttggcg agcggccgtc tcgagctcct tgcccgcgcg cgtttgctcc gcgatgccgc
      601 gggcggcgag cgcgacgtcc tttcgcgcga cgtccatcgc gcgggcgatt tcgctcgcgc
      661 ccttggattg ctctgcgagg gccgtgctcg tgcgctccac ggcgacgctg ccttcttgta
      721 tttcggcggc cacggcgttg atgaggctcg tctgctgcgt gattgagccc gcggtcgctt
      781 gcgtcgtcgt tcgcatttgg gcggcaacgc gcgacaagtc ggaggcgctg gccgcctgtt
      841 ccgaagtggc gtgcgcgact tgcttcgcgc tggccgcgag ttggtcgttg gctttcacga
      901 cgtcccgcaa ggctcgcgct tgctccatcg acgcttgcgt actttgcttg gccatctggc
      961 gcacttcggc cgcggtccgg gacaactcgt cgtgcgcctt ggcttgctcc tgaatcgcct
     1021 gcgcgatctc gcgggttcgc tcgcgtacct gattcgctac actggacaac gagcgcgccg
     1081 cgtccacttg ctcgctactc gccgcgaata cttcgcgcac ggagccaccg agctccccga
     1141 tactgccgac gatgctggaa agggcagctt gcgcctccgc ggcgagcgcg ccgccctcat
     1201 tggcaacgcg ttcgccttcg acggtcgtgg tgagcgcgtc acgcgcggtg ttctgcagcg
     1261 agcgaatcac cttggcgatg tccgagctcg ccgtcgcggc tcggtcggcc agcgcccgaa
     1321 tttcgtccgc gacgaccgca aagccgcgac catgctcgcc cgcgcgcgcg gcttcgatgc
     1381 tggcgttcag cgagagcaaa ttggtgcggt ccgcgatcaa gttgatcgtc tgcacgaact
     1441 cgccgatttc gtcggctcgt cgcgtcatct cgcgcatgac accagccgag gacgtgagcg
     1501 aatcgcgaat cttcgacagc ccgtggatcg actttcccac cgtggcgccg ccgtcgcgcg
     1561 ccagcgtcgc cgcgcgctcg ctcgcgacct gcgcgccctc ggtcactttc gcgattcgtt
     1621 gcgtcgactt ttccaactgg gaggcagcgg ttgcgctgct ctccgacagt cgccgtattt
     1681 cctccgcgct gcgggtgacg gaggacgacg acttggccat ctgttccatc gtcgtggcgt
     1741 tgctgtcgat cgcgcggctc gttcgttcgg agctgaccgc cacctgttcc accgaagcgg
     1801 cgatctcggc gagtccggct gcgttctcca tgctcgctgc ttcgagcgtt ctcgcgtgcg
     1861 tggccacccc cgccacggaa cgcgccattt gttcggcggt gctcgcggtc tgagccaccg
     1921 cgctcgcgag cgcctgggct tggtcgctga cttctgcgcc gcttgccctg agggcatgca
     1981 tgctttccat cgccgaagcc atgcgctgac gcatgtcgcg cgcgtcgctt gccagtcgtt
     2041 cgataccctt cgccatttgc tcggcggtag cgccgacctc ggccacgttg ccggcgtttg
     2101 actcacttcc gccggcgagg gataccaacc cggctgccat ttcggtaatc gtagcgagca
     2161 actgctcggt cgcgctcgcg gtctcgtccg aattggcggc gacgcttcgg atcgagcggg
     2221 ccgactcttc gatggtcgcg gccatcgact cgctcgagct cgcggcgttc tcggcgtctt
     2281 tcgtgattgc ttccgcctcg cggctgacgc gtaccagaag ccgcgatccc tcttcggtgc
     2341 tctgactcag cgctcgagtc gccgcgtcga agtcggcctg tccgcgcgtc agcttcatca
     2401 gcatctcgag cgtgtcttcg atgctggcgg cggcggcctg gagcgccgcg tgcgtctgct
     2461 gactcgctgc cgcgcgctga cttccgctcg tggccgctcg cgtcaatgat tcggcgagtg
     2521 ccgacgtatc ggcggcggtt tgccgcagtg agctaacggt cgcctcggct tgggagcgcg
     2581 tgaggtccgg tagcggagct gttcgggatg aggcggcgtt gttcgtcgtc atggtgttcg
     2641 tcttcttcgc cgaggagcct ggagagagcc aaaatcaaga gcaatcgctg ccccaacatg
     2701 caaacggagc tcacataagc agccgcttcg tgtgcggcca gggcgggcgc gctctggaac
     2761 tgactgggtt ctagtttgag aacctcgcgc gcactgtcga cccgcagcgc gaccacgcgc
     2821 gcaccacgac gcgtgacgag gacgcgtgtg tcccgagtcg gcgcgactcg ttcctggccg
     2881 aacagcgtac gcaagtcgag cacgggcatc accttcccac gcacggctac gatcccttcg
     2941 acgtggggaa gcgtactcgg cacttccgtc gctccactga aggcttcgag ctcgagcacg
     3001 tcgctcgcgg cgagagcgta ttcggtcccc gcgactacga acccaacgta gagcgacgtc
     3061 acgacgggct ctccgcccgg tggcgccccg cgctcaagac ttcgctgaac gcgatcaagt
     3121 ccagcaccag cgtgggcttg ccgtcgccga ggtcggtggc gccggtcacc ccgggcaagc
     3181 ggatcaacgg atcttcgatg gggcgaacta cgacctcttg ctgacccaac atgcggtcga
     3241 caccgaaggc gagggcggcg cccgcgcgct tgaccacgat ggccttgggg ctcgcttcgg
     3301 cggcgcccca gttgaaaagc tcgcgcagca tgacgatcgg gatgcttcgg ccgcgtcgcg
     3361 cgatgactcc aaccgcaggc ccgcggtcgt ttgcgcgggg tccgtagacg atctcgtcgg
     3421 ggtcgacgtg aactatctcc tccaccgtcg acacgggcac gacgaagcgc tgggcgcgac
     3481 actcgaacag gaacgcatcc acgatcgaga gcgtcagggg tactcgcagc gtgaactttg
     3541 ttccggcgcc ccaggccgtt tcgagctcga gttctccgcc gagctcgccg acgacgatcc
     3601 gcttcacgat ttccatgccc attccgcggc cgctcgtggt gttcgcgact tgacgcgtcg
     3661 agaagcccgc aacacagagg aggtcgagca gctcgctcgc gctctgcgga gcggggc
//
LOCUS       DASTKS010000716         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_337697, whole genome shotgun sequence.
ACCESSION   DASTKS010000716 DASTKS010000000
VERSION     DASTKS010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337697"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFQ35_14830"
     CDS             <1..306
                     /locus_tag="VFQ35_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931974.1"
                     /translation="VEVGWEPPRGPGVRLASRLGVSLLSTEPASGFAASPTAVRTAAF
                     LELDVQRPIWFGAIGVAPGVGVRVFSAPRTVNVDGEQALRLAAPTPRVTVALLLQTN"
     gene            428..1747
                     /locus_tag="VFQ35_14835"
     CDS             428..1747
                     /locus_tag="VFQ35_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931975.1"
                     /translation="MSEVMEMQGGLVEGSGDEQPAESAELVQAPTKSFESYPYRFYVA
                     LDGNGVNGLEGMAGVCSFLFDPADGSYAHKIRFFDGAAAGHAVSVNPDGSVGFLGNAA
                     QHLLFYDARSLEEVGRVSTLRFEPPSSSLQGSTHVVWLSDREFVTAIGRHFYRFSLDR
                     LECPERLAEHKVKLPHGMKRTASGHFVVYGSMDNPAEGAQGEARHVGILDLRSGEARV
                     VALPATCWHVAVHPKEDVFYAISFRVAPQDHVDWHDWGMAFLKEYAFEIDVASGTVQR
                     HWAAGREVPAHINSDICLSDSELIFCNGGSQSIVCVDLASFARYRFIDEKPDAQAQLD
                     RPREVRTQVFDVLARGNLFTNARHLLGALRVSRFSLLDSVYACQLSKDQRLLFTANRG
                     LNHISIYDYPEATLRVRVKMPSIQEHFSWMSELDDPRLGFHHGHLIG"
     gene            complement(1817..2413)
                     /locus_tag="VFQ35_14840"
     CDS             complement(1817..2413)
                     /locus_tag="VFQ35_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931976.1"
                     /translation="MTNFDSFTMVREPLEVVWTTLRDDLSRVARQLEDLESIDTVERE
                     VSETGVWLLNRWTARQKVPALLARYLGTDRIAWEDRAFWRDASKCCEWSIRPTLLPEH
                     VECSGVTRYEPVMAGRGTRVSFRGSFTLKPGFGGALPAALEPTIASFAESIISTLIPR
                     NLARAVQVAGQTIAAERAPRGELAGPVESAPHQPLQRE"
     gene            complement(2397..>2990)
                     /locus_tag="VFQ35_14845"
     CDS             complement(2397..>2990)
                     /locus_tag="VFQ35_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931977.1"
                     /translation="EVLFACPTMTVGTQATALGPSNAAILAYLADPLRLTYPGGCNFV
                     AAPDVAVGHRLIALAGRPGEHYLLGSENATWEQIHRLIAELCGVPAPRAHINHSAAFL
                     AATFEELRAPLQGRKPLTTREQARMVGRYYWYSHAKAAALGYRPAPLRAALAAACAWL
                     VASPHVSRELRGQLRLHAEVHAARSAQRAGRTHDELR"
BASE COUNT          507 a          920 c         1003 g          560 t
ORIGIN      
        1 gtcgaggtcg gctgggagcc cccgcgcgga cccggcgtgc gactcgcttc gcgcctcggg
       61 gtgagcttgc tgtcgaccga gccggcgagc ggcttcgcgg cgtcgccaac ggcggttcgc
      121 acggctgcct tcctagagct cgacgtgcag cgcccgattt ggttcggcgc catcggcgta
      181 gctcccggcg ttggcgtgcg agtcttctct gctccgcgga cagtcaacgt cgatggagag
      241 caagcgctgc gcctcgccgc gccgacgccg cgcgtgactg tcgcgttgct cctgcaaacg
      301 aactgagcgt ttggcggtgg aaaaggtggt cgggtgaatg tttcgaacca tcgtggtgag
      361 cggtggtggc tggctgccga ggttgacgcg gagcgcgggt taaaagcaca ctgacgccgc
      421 gaagatcatg tccgaggtga tggaaatgca gggaggtttg gtggagggca gcggagacga
      481 gcagccggcc gagagcgccg agctcgtgca agctccgacc aagtcgttcg agagctatcc
      541 atatcgcttc tacgttgcgc tcgacgggaa tggcgtgaac ggcctcgaag gcatggcggg
      601 agtgtgctcg ttcttgttcg atccggcgga cgggagctac gcgcacaaaa ttcgcttctt
      661 cgatggggct gcggctgggc acgcggtctc cgtgaatcca gacggcagcg tcgggttctt
      721 gggcaacgcc gcacaacact tgctgtttta cgacgcgcgg agcctcgaag aggtgggccg
      781 cgtttcgact ctgcgcttcg agccgccaag ctcttcgttg caaggttcca cccacgtggt
      841 ctggctatcg gatcgtgaat tcgtcaccgc gatcggccgc cacttttacc ggttttcact
      901 cgaccgcttg gaatgtcccg agcgcttggc agagcacaag gtcaagttgc ctcacggcat
      961 gaagcgcacc gcttccggcc atttcgtcgt ctatggctcg atggataacc cggccgaagg
     1021 tgcgcagggc gaagcgcggc atgtcgggat cttggacctg cggagtggcg aggcgcgcgt
     1081 ggtcgcgttg ccggcgacgt gttggcacgt ggcggtgcac ccgaaagaag acgtatttta
     1141 tgcgatctct ttccgggtcg cgccacagga tcacgtcgat tggcacgatt gggggatggc
     1201 gttcctcaaa gagtacgcgt tcgagatcga cgtcgcgagc gggaccgtcc aacggcactg
     1261 ggcggccggg cgcgaagtgc ccgctcacat caactccgac atctgcttgt ccgacagcga
     1321 gctgatcttc tgcaatggcg gtagtcagtc gatcgtgtgc gtcgatctcg cgagctttgc
     1381 ccgctaccgt ttcatcgacg agaagccgga cgcgcaagcg cagctcgatc gccctcggga
     1441 ggtgcgcacg caagtgttcg atgtgttggc gcgtggcaac ttgttcacca acgctcgcca
     1501 tctgctcggt gcgctcaggg tgtcgcgctt ctccttgttg gactcggtct atgcctgcca
     1561 actatcgaag gatcaacggc tcctgttcac cgcgaaccgc gggctcaacc acatctcgat
     1621 ctatgactat cccgaggcga cgttgcgcgt acgggtgaag atgccgtcca ttcaagagca
     1681 cttctcttgg atgtcggagc tcgatgaccc gcggctcgga tttcatcatg gtcacctaat
     1741 cggttgagct cgacgccgcg ctcgcgacgt tcgcacgaac tcgagcgtcg agtgatggct
     1801 cacttcgcgc ccgtggtcac tctcgttgca ggggttggtg cggcgcgctc tcgactgggc
     1861 cggcgagctc gccccgtggc gctcgctccg ccgcgatcgt ctgcccggcg acctgcacgg
     1921 cacgggcgag attgcgtgga atcagcgtgc tgatgatcga ttcggcgaac gaggcgatcg
     1981 tgggctccag cgcggcgggc agagcgcccc cgaaccccgg cttgagggtg aacgagcctc
     2041 ggaagctaac gcgggtgccg cgtccggcca tcaccggttc atagcgcgtg acgccggaac
     2101 actcgacgtg ctcgggcaac agggtcgggc gaatcgacca ttcgcagcac ttgcttgcgt
     2161 cgcgccagaa tgcgcgatct tcccaggcga tgcggtccgt tccgaggtag cgagcgagca
     2221 gcgccggtac cttttgtcgc gctgtccaac ggttcaagag ccatactccg gtctcgctga
     2281 cctcgcgctc gacggtatcg atgctctcca gatcttcgag ctgtcgagcg acgcggctca
     2341 ggtcgtcacg cagagtggtc cacaccacct ccagcggttc acgtaccatg gtgaagctat
     2401 cgaagttcgt catgggtcct ccctgcacgc tgcgccgaac gcgctgcatg aacttctgcg
     2461 tggagccgca actgaccgcg gagctcccga gaaacatgag ggctcgccac cagccacgca
     2521 cacgcggcgg ccaatgccgc gcgaagtggc gcgggacgat agccgagcgc ggcggccttg
     2581 gcgtgggaat accaataata cctcccgacc atgcgcgctt gttcgcgcgt ggtgagcggc
     2641 ttccggccct gtaacggggc ccgcagctct tcgaaggtcg cggccagaaa agcggctgaa
     2701 tggttgatgt gcgcgcgggg agcggggacg ccgcagagct ccgcgatgag acgatgaatc
     2761 tgctcccacg tcgcgttctc actcccgagc aagtaatgct caccgggacg cccggccagc
     2821 gcgatcaagc gatgaccgac cgccacgtca ggcgccgcga cgaagttgca gccaccggga
     2881 tacgtgagcc gaagcgggtc tgcaaggtac gcgaggatcg cggcgttcga gggaccgagc
     2941 gcggtagcct gagtgccaac cgtcatcgtc gggcaggcga aaaggacttc
//
LOCUS       DASTKS010000717         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_114891, whole genome shotgun sequence.
ACCESSION   DASTKS010000717 DASTKS010000000
VERSION     DASTKS010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114891"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1861)
                     /locus_tag="VFQ35_14850"
     CDS             complement(206..1861)
                     /locus_tag="VFQ35_14850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9931978.1"
                     /translation="MVAETDATGFSPGYDPLLGTVVGGRFRIENRIARGGMSSVYFAV
                     QAPLNRPVALKVLRGSLGEDAESADGFRRRFLQEASILSQLSHPNIVTLLEYGQVREL
                     PEQPYFMAMEYLRGETLAQRFRARGRLGVDECIRLARQIGRGLRQAHRRGFVHRDLKP
                     SNIMLVPEDDGHDIVKLVDFGIGKILRGPLERDVEDQTRVGLLLGSPRHMAPEQIRCE
                     PVEVRTDLYGLGVILFQALTGRLPFDGRTEIDVLMAHCSMPAPALNEACPEQPFPESL
                     ASLVQSLLAKSPSGRPSVDEFLQSLSEIEQELFGRVGLAGPTSRHVAPTLRSPTRAGA
                     ESEDHTEVTLAEWRSQDPPLTGGAGVVSLAPHSLAAPRPKKRGRWAVAGAVVALAGVA
                     GFTLQNLRAQRAESAVKARSQPAAVVPARVARATPPVPDDAPTSSFALTISSSPTSAT
                     VSENGAPIGTTPLSLSIARDSVAGQKRRFVIEQEGFAPYTIEQGDSATSVSVLATLVA
                     SARKSARSRVSRGNSGGTIPTRAGEGFAAKRNDAINLDIRPRR"
     gene            complement(2097..>3634)
                     /locus_tag="VFQ35_14855"
     CDS             complement(2097..>3634)
                     /locus_tag="VFQ35_14855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9931979.1"
                     /translation="AAPRLSRRQVVAAASASALTLALPRPAFSKGQPSVAIVGAGIAG
                     LTCALKLADRGIASTVYEASGRAGGRMFSNSNYWAQGQVSEWCGELIDSGHETVQALA
                     KRFRLQLDDLHAAEPEGARDTYRFFNDYYTQSKAEADFLARVAEAVAADAEAADYPTH
                     FDSFTDAGALLDSMSVYDWIESRVPGGHRSRLGALLDIAYNTEYGAETTDQSALNLVY
                     LLGFQPDEAGFSVFGESDERYHIRGGNQQLPNKMAEHLGNVAYGHSLRRVKRTAAGRY
                     ALTFGRGAGSSVEVVADYVVLALPFAVLRGIDVRDAGFDALKLEAIQELGRGRNGKTQ
                     LQFSQRIWNGKGSWPGISNGSSYSDTGYQSSWEVTRAQPGTNGILVFYSGGKVSSAMA
                     TQRPFATVSDPGVLADAATALRRAEPVFRGLSSVWTGRATQSLPHESEFFKASYAYYR
                     VGQYLSFAGYEGVTQGGVLFCGEHTSQDFQGFMEGGASEGKRAARELAQLIRGRVDEL
                     DAI"
BASE COUNT          645 a         1206 c         1183 g          600 t
ORIGIN      
        1 gcgtgaggat agtcgcccgc ctgatagcgc tccgctccga gcgtgaacat caagtcggct
       61 tcgtcggcca cgtcgtcggc tcgcgcctcg aacgaccagc agacgctcag cgacaaacaa
      121 gcggtcaaga gccagcgcca cgagcggcgc ctgatgtgcg ttttcgggcg ggcaggcaca
      181 gtgcggccag aaataccgca gccgctcagc gccgtgggcg aatatcgaga ttgatcgcgt
      241 cgtttcgttt ggccgcgaag ccttcgcccg cgcgcgtcgg tatcgttccg cccgaattgc
      301 cgcgcgacac ccgcgaacgc gccgattttc tggcgctcgc gacgagcgtg gccaaaacgc
      361 tgacgctcgt cgcggagtcg ccttgctcga tggtataggg cgcaaagcct tcctgctcga
      421 tgacgaagcg gcgtttttga cccgcgaccg aatcgcgcgc gatgctcagc gaaagcggcg
      481 tggtcccgat gggcgcgcca ttttcgctca ccgtcgcgga cgttggcgac gagctgatcg
      541 tcaaggcgaa agagctcgtg ggagcgtcgt cggggacggg gggcgtagct cgagcgactc
      601 gagccggaac gacggccgct ggctgcgagc gcgccttgac cgcgctttcg gcccgctggg
      661 cgcggaggtt ttgcagcgta aacccggcga caccagcgag cgcgacgacc gcgcccgcga
      721 ccgcccagcg accgcgcttt ttgggccgcg gcgccgcgag tgagtgcggc gccagcgata
      781 cgacccccgc cccacccgtc aacggcgggt cctgcgagcg ccactcggcc agcgtcacct
      841 ccgtgtggtc ctcgctctcg gcgccagctc gagtcggaga ccgcaacgtc ggcgcgacgt
      901 ggcgagacgt cggccccgca aggccaaccc gaccgaagag ctcttgctca atttcactca
      961 aactctggag aaattcgtcc acgctcgggc gaccgctcgg gctcttcgcc aagagagatt
     1021 gcacgagaga agcgagggac tccgggaaag gctgctctgg acacgcttcg ttcaaggcgg
     1081 gagcaggcat cgaacagtgc gccatgagca cgtcgatttc ggtgcgaccg tcgaacggaa
     1141 gtcgacccgt gagcgcctga aaaaggatga ctcccaaccc gtataggtcg gtcctcacct
     1201 ccacgggctc gcaacgaatt tgctccggcg ccatgtggcg cgggctgccc aagagcaacc
     1261 ccacgcgggt ttggtcctcg acgtcgcgct cgaggggacc gcgcaagatc tttccgatcc
     1321 cgaaatcgac gagcttcacg atgtcgtgcc cgtcgtcctc ggggacgagc atgatgttcg
     1381 acggcttgag atcgcggtgc acgaagccgc ggcgatgcgc ttgcctgagg ccccgcccaa
     1441 tctgtcgagc gaggcgaatg cactcgtcga cgccgaggcg gccgcgagct cgaaaacgct
     1501 gcgccagcgt ttcgcctcgc aggtactcca tcgccatgaa gtacggctgc tcggggagct
     1561 ctcggacttg cccgtactcg agcagcgtga cgatgttcgg gtgagacagc tgcgaaagga
     1621 tgctcgcttc ctgcaggaac cgtcgccgaa agccgtcggc ggattcggcg tcttcgccga
     1681 gcgagccgcg caagaccttc aacgcgacgg gtcggttcag cggcgcttgc accgcgaagt
     1741 agacactcga cataccgcca cgcgcgatcc ggttctcgat gcgaaagcgt ccgccaacga
     1801 ccgtccccaa gagagggtcg tacccgggag aaaaccccgt ggcatccgtt tcggccacca
     1861 tcctgcgctc gttacgcgaa ggtcgtatct cgcttcgagc ccaaactcaa aatcaatttg
     1921 ccgcaatcgg agcgcgcgtg tgcgcatgca gaggcgcgtc tcgccgcttc cgtgcgcgtt
     1981 cgacgcaaag tgcggaacaa gtgctcagca gatgtctagc gggtttcgcc gaccgagcgc
     2041 gcggacggtt ctcgcgtcct gcacgcgcgc aatcggcgaa gtgagccagg tttcggtcag
     2101 atcgcgtcga gctcgtcgac gcgaccacgg atgagctgcg cgagctcacg cgccgctcgc
     2161 tttccttcgg aggcgccacc ttccatgaaa ccttggaagt cttgtgaggt gtgctcgccg
     2221 cagaagagca ctcccccttg ggtgacgccc tcataacccg cgaaagagag atactgcccg
     2281 actcgatagt aggcgtacga cgccttgaag aactcgctct cgtgcgggag agattgcgtc
     2341 gcgcgcccgg tccaaaccga gctcaacccg cgaaataccg gctcggcgcg ccgcagagcg
     2401 gtggccgcgt cggcgagcac tcccgggtcg ctcacggtcg cgaacgggcg ctgcgtggcc
     2461 atggcagagg atacctttcc acccgagtag aagaccaaaa tcccgttggt ccccggctgg
     2521 gcgcgggtga cctcccaact cgattggtaa ccggtgtcgg agtagctcga gccgttgctg
     2581 ataccgggcc aggagccctt gccgttccaa atgcgctgcg agaattggag ctgggtcttg
     2641 ccgtttcggc cccgaccgag ctcctgaatt gcctcgagct tcagcgcgtc gaagcctgcg
     2701 tcgcggacgt cgattccgcg caagaccgcg aacggcaacg cgagtacgac gtagtcggcg
     2761 acgacctcga cagaactgcc agcgccgcga ccgaaggtga gcgcgtaacg acccgccgcg
     2821 gtgcgcttga cgcgtcggag cgaatgtccg tacgccacgt tgccgaggtg ctcggccatc
     2881 ttgttcggca gctgttggtt gccgccgcga atgtggtagc gctcgtcgga ttcaccgaac
     2941 acggagaacc ccgcttcgtc cggctgaaat ccgagcaagt agacgaggtt gagagcagac
     3001 tgatccgtcg tctccgcgcc gtattcggtg ttgtacgcga tgtcgagcaa cgcccccagc
     3061 cgcgaacggt gcccgccggg gacgcgcgat tcgatccaat cgtagacgct catcgagtcg
     3121 agcagcgccc ccgcgtccgt gaacgagtcg aagtgcgtcg ggtagtccgc ggcttcggcg
     3181 tccgccgcga ccgcctccgc gacgcgcgcg aggaagtccg cctccgcttt cgactgcgta
     3241 tagtaatcgt tgaagaagcg atacgtgtca cgcgcgccct cgggctcggc ggcgtggaga
     3301 tcgtcgagct ggagacggaa acgcttggcc aaggcctgca cggtttcgtg gccgctatcg
     3361 atgagctctc cgcaccactc gctgacctga ccctgcgccc agtagttcga gttcgagaac
     3421 atgcgcccgc ccgcgcggcc cgacgcctcg tacaccgtcg atgcgatacc ccgatcagct
     3481 agcttcagcg cgcaggtgag ccccgcgatt cccgcgccga cgatcgcgac gcttggctgg
     3541 cccttcgaga atgccgggcg cgggagagcc aacgtgagcg cggacgcaga ggcggcagcg
     3601 accacttgcc gtcgcgacag tcgcggcgct gcct
//
LOCUS       DASTKS010000718         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1901481, whole genome shotgun sequence.
ACCESSION   DASTKS010000718 DASTKS010000000
VERSION     DASTKS010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901481"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..321
                     /gene="moaD"
                     /locus_tag="VFQ35_14860"
     CDS             73..321
                     /gene="moaD"
                     /locus_tag="VFQ35_14860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET9931980.1"
                     /translation="MTLTVLYFAKARELAGTSEERIQFTAEPPTLRRLMQELTQRHPL
                     LAGALECMRVARNEEFAELDDVAAENDVIALIPPVAGG"
     gene            complement(379..1815)
                     /locus_tag="VFQ35_14865"
     CDS             complement(379..1815)
                     /locus_tag="VFQ35_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9931981.1"
                     /translation="MPKFSSLLACLTSSLSSPPGRMDSSSGAVVDVARRRAARGVARL
                     SRPVKLVGAAGLVLTAMSAGCGLTSGDDDSGNGNGSGGQSPISFGGSSQASGGFDPIG
                     FGGGSSVAGAIGSGAANGFDGGLLPITPEQKKLVTDAACAGFLTEGESLPSVLELVVD
                     TSSSMRNMAPGNPGMSKWDVTRDALLEAVVGVTGSGLPASVAVGVLFYPNVEYQVKSS
                     QQPLETCVNTRAAVPIDLLGGKGAAHRTAIGNAISQVQLAQSTPTADAYGYALESQLL
                     PSKALGKKFMLLITDGTPTLMKGCYNPSGELNDVDPTPIVEEITGAAREGIETFLIGS
                     PGSEANRTWMSRAAVIGGTANAGCNVNGPNYCHMDMTTEPDFAAALRAGLARVVGQIT
                     PCTFSFAVPPAGQTIDPKNINVIINSSGQSSLVVRDDIGDCTQGWQLTPDNEILLCPD
                     TCATVKSDSGASVDVVFGCGALTEVPVK"
     gene            complement(2071..3000)
                     /locus_tag="VFQ35_14870"
     CDS             complement(2071..3000)
                     /locus_tag="VFQ35_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9931982.1"
                     /translation="MANGGAGGASAGRGGDAESGGAAGGGAPPSEGGAPPCEPVGESD
                     PHATTRFAIFGDYGSVGEAEAGVAELVKSWNVDFIATAGDNNYPLGEAETIDANIGQY
                     FSEFICPYRGSYGSGATKNRFFPALGNHDWYTAGAEPYLEYFELPGNERYYDFVWGSV
                     HLFMLDSDPSEPDGVMSDSRQAEWLSRGLAASTARWKIVVMHHAPYSSSQHGSNSYTQ
                     WPYKEWGADLVFAGHDHTYERLHVDGLTYIVAGLGGASTYGFTTTVLGSRAQYNAGYG
                     AGLVEADATHLWFRFVGIDGSLIDEFTIASPAW"
     gene            3573..>4607
                     /locus_tag="VFQ35_14875"
     CDS             3573..>4607
                     /locus_tag="VFQ35_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931983.1"
                     /translation="MRRLALKFGVAANVLSLLAACSSSDGATNPGSVHEETCRVSDPK
                     NSPDYIQHIGCKADFDALASVPLDATIPGARSVKVIVDQRDDQYPNELYFQNSTRFKI
                     HFEFASTHLSGKGPKDQTLPTVLGIADFSSTEYYKNTRRFVLGAVTYYEGPKVWALEI
                     APYDTASPEMIELLYSRVKAATYFGSELRFHPTSDSVLATSKKLASTVNVVTTDEIYA
                     GTDYQPLNLATTIGKVRFLTADTLDSNYVGFQDIVVLDHVPNDISVVAGLVTEEFQTP
                     LSHVNVLAQTRGTPNMGLRKALTNTKLRALEGKWAKLTVGASSWDAVEVTQKEADDWA
                     NAHKPAPITLP"
BASE COUNT          865 a         1546 c         1399 g          797 t
ORIGIN      
        1 tcgagtgtcg cccatccacc gacatgactt gcgacgaggc acaacgtgcg acatggtcgc
       61 ttgcgcgcag caatgaccct caccgttctc tactttgcca aggctcgcga gctcgcaggc
      121 accagcgaag agcgcattca gttcacggcc gagccgccga cgctgcggcg actgatgcaa
      181 gagctaaccc aacgtcaccc tctgctcgcc ggagcgctcg agtgcatgcg ggtcgcacgc
      241 aacgaggagt tcgccgagct cgacgacgtc gccgccgaaa acgacgtcat cgcgctcatt
      301 cctcccgtgg ctggcggctg agcggcccgc gaactttcga gtcagtcgca atgcggcggg
      361 tcgtgtgccc gccgccgttc acttgacggg cacctcggtg agcgcgccgc agccgaacac
      421 cacgtccacg ctcgcgcccg aatcggactt gaccgtcgcg cacgtgtccg gacagagcaa
      481 gatctcgttg tccggggtga gctgccagcc ttgcgtgcaa tcgccgatgt cgtcgcgaac
      541 gacgagcgaa gactgcccgc tcgagttgat gatgacgttg atgttcttcg ggtcgatggt
      601 ctgacccgcg ggtggcaccg caaagctgaa cgtgcacggc gtgatctgcc cgacgacgcg
      661 cgcgagccca gcgcgcagcg cggccgcgaa gtctggttcc gtggtcatat ccatgtggca
      721 gtaattgggg ccgttcacgt tgcagcccgc gttcgcagtg ccgccgatga cggcagcgcg
      781 cgacatccac gtgcggttcg cttcgctacc gggagacccg atcaagaacg tctcgatgcc
      841 ttcgcgcgcg gccccggtga tctcctcaac gatcggcgtc gggtcgacgt cgttcagctc
      901 gccgctcggg ttgtagcaac ccttcatgag agtgggcgtc ccgtccgtga tgagtagcat
      961 gaacttcttg ccgagcgcct tgctcggcag gagctgactc tcgagcgcat agccgtacgc
     1021 gtctgcagtc ggggtgctct gcgcgagctg cacttggctg atcgcgttgc cgatcgcggt
     1081 gcgatgcgcg gctcccttgc caccaagcag atcgatgggg acggccgccc tcgtgttcac
     1141 gcacgtctcg agcggttgct ggctcgattt cacctggtac tcgacgttgg ggtagaagag
     1201 tactcccacc gcgacgctcg ccggcagccc actgccagtt acacccacga ccgcttcgag
     1261 caacgcgtcg cgcgtgacgt cccatttcga catcccgggg ttgcccggcg ccatgttgcg
     1321 catcgagctg ctcgtatcga cgacgagctc gagcacagaa ggcaaagact cgccctcggt
     1381 caggaaaccc gcgcaagcag cgtcggtcac gagcttcttc tgctcgggag tgatcgggag
     1441 gagcccgccg tcgaaaccat tcgccgcgcc gctgccgatc gccccagcga cggagctccc
     1501 gcccccgaac ccaatcggat cgaagccgcc gctggcttgc gagctaccgc cgaagctgat
     1561 cggcgattgc ccgcccgaac cgttgccgtt accggagtcg tcgtcgccag acgtgagccc
     1621 gcagcccgcc gacatcgcag tgagcacgag gcccgccgcg cccacgagct ttaccggtcg
     1681 cgagagccgc gcgacgccgc gggccgcgcg acggcgagct acatcgacga cggccccgga
     1741 actcgagtcc atccgcccag gcggactcga aagcgaggac gtcaggcacg cgaggagaga
     1801 agaaaacttc ggcatggtaa tggaacctat cgcatcgagc tgaaacgcga agcctacgaa
     1861 ggacgagaaa tagctccgtc ccaggtgacg ccgcggaagc ggtacctgcg cggttcagtc
     1921 gtgcgcgcgc tcacatagtg caccgctcga gagacgagcg caatcgttgc aagcagcgct
     1981 ggcttcgtcg cgcgggggtc caccacccgc cggtccgagt gggtgctccg ccccgttacc
     2041 aagctccccc tctctgacga tcacgcacat tcaccacgcg ggtgaagcaa tcgtaaattc
     2101 gtcgatcaag ctgccatcga tgccgacgaa tcgaaaccaa agatgagtcg cgtcggcttc
     2161 caccaaccca gcgccgtagc cggcgttgta ttgcgcacgg ctgccgagga ctgtggtggt
     2221 gaacccatag gtgctagcac cgcccagccc ggcaacgatg tacgtcagac cgtcgacgtg
     2281 cagccgctcg tacgtgtgat cgtggccagc gaatacgaga tccgcgcccc attctttgta
     2341 aggccactgc gtgtacgaat tcgaaccatg ctgcgaagac gagtacggcg catggtgcat
     2401 cacgacaatt ttccagcgcg cggtcgaggc cgctaggccc cgcgatagcc actcggcctg
     2461 gcgcgaatcg ctcatgactc catccggctc gctcgggtcg ctgtcgagca tgaatagatg
     2521 cacgctgccc cacacgaagt cgtagtagcg ctcgttaccc ggcagctcga agtattcgag
     2581 ataaggctcg gccccagccg tgtaccagtc gtgatttccg agtgcgggaa agaaacggtt
     2641 cttcgtggcg cccgagccat agctcccgcg ataggggcag atgaactcgg agaaatactg
     2701 tccaatgttc gcgtcgatgg tttcggcttc accgagcggg tagttgttgt cgcccgcggt
     2761 agcgatgaag tcgacgttcc aactcttcac gagctcggcg actcccgctt cggcttctcc
     2821 cacgctgccg tagtcgccga agatcgcgaa gcgggtggtc gcgtgcgggt cgctctcgcc
     2881 cactggctca caaggcggtg ctcccccttc cgacggcggt gctcctccac cggctgcgcc
     2941 gcccgactcg gcatcccctc cgcgtcccgc gctcgcgcct ccggcaccac cgtttgccac
     3001 cgccccgccg agaccacgca ggccgccgct cgcgctggct ccgccggtgg cctcaggctg
     3061 cgctcgcgcg tctgcctcgg ttcgggagcg cgagggtgct gcgctggccc gacccggaaa
     3121 ccccctgctc gctatccttg cgtcgtcaca gctcgccagc agccccacca ccgtgagcga
     3181 gacgagagtc cgcgaaaccc tggagagggg aacggctact gaggcgatgt gacgacagtg
     3241 tcccgcccaa tgcgaaatca ggcgtcgcgg gaggaatttc cggttcatcg gtatcgtccg
     3301 cttggaaaat tctcgctaaa gcgcgtgccc gtggtcatct caaagtgacg aatgcgcgac
     3361 taaatagaaa acacctgcaa gctttttggc aatctaggtg actgagtcgg tcatgacgag
     3421 ctagtcgggc cgccgtaaac cctggaaact ccgaaagtcc ggccccccgt acaggcacga
     3481 cgctcacact tggcgacaac tgtatttctc ggcgccgcgt gagctcacga ggacagatgc
     3541 gaacagttac taacgagact ctctgtaaga atttgcgtcg actagcattg aagttcggcg
     3601 ttgcggcgaa cgtgctctcg ctgctcgcag cttgcagctc ctcggatggt gcgaccaatc
     3661 cgggaagcgt gcacgaagag acctgtcggg tgtcagatcc gaagaactct cccgattaca
     3721 tccagcacat cgggtgcaaa gcggacttcg acgcgctcgc gtccgttccg ctcgatgcga
     3781 cgatcccggg tgcgcgttcg gtcaaggtga tcgtcgacca acgcgacgac cagtatccga
     3841 acgagctcta cttccagaac agcacgcgat tcaagattca tttcgagttc gcgtccactc
     3901 acctctcggg taagggaccg aaggatcaaa ctctgccgac ggtgctgggc atcgccgact
     3961 tcagctccac cgagtactac aagaacacgc gccgcttcgt gctcggcgcc gtgacgtact
     4021 acgagggccc gaaggtctgg gcgctcgaaa tcgctcctta cgacaccgcg tcgcccgaga
     4081 tgatcgagct cctctactcg cgagtgaaag cggcgaccta tttcggctcg gagctgcgct
     4141 tccacccgac gtccgactca gtgctcgcga cgtcgaagaa gctcgcgtcg accgtgaacg
     4201 tcgtgaccac cgacgagatc tacgccggca cggactacca gccgctgaac cttgccacca
     4261 ccatcggcaa ggtgcggttc ctcaccgccg acacgctcga cagcaactac gtcgggttcc
     4321 aagacatcgt cgtcttggat cacgtgccga acgacatctc ggtcgttgcg ggtctcgtca
     4381 cggaagagtt ccagacgccg ctctcacacg tgaacgtcct cgcgcaaacg cgcggaacgc
     4441 cgaacatggg gctgcgcaag gcgctcacga acacgaagct ccgcgccctc gagggcaagt
     4501 gggccaagct caccgtcggc gccagcagct gggacgcagt ggaggtgacc caaaaggagg
     4561 cggacgactg ggcgaacgct cacaagcctg cgccgatcac gcttccc
//
LOCUS       DASTKS010000719         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1677503, whole genome shotgun sequence.
ACCESSION   DASTKS010000719 DASTKS010000000
VERSION     DASTKS010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677503"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2645
                     /locus_tag="VFQ35_14880"
     CDS             <1..2645
                     /locus_tag="VFQ35_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9931984.1"
                     /translation="ASEDAPGEPLGTPTYVVTEMLSHDAGIEQVSTTPLRARARQLEQ
                     LERAFQDAQRGAVVTRLIVGIAGAGKSRMLHEWRRRSWQATDLLLTAYATVHEHHSPF
                     APLLRMWRSWFGFPLDDPPQAIRERLQTVLEQAELPVEKFAAPLAYAFGAGDDIVSKQ
                     PAERRRHLVIQAFVELTIALAEKRLVVLAFEDLHFVDRSTLDVLRRLVESARPARLLL
                     VLTARPEFVVGWPLANRISRIDVTPLGQSDALQLVESMSQRSSLPTALVRRIVDLSDG
                     VPLVLEELVRAALSDWERDGEVADLMSRPPTSFADSVARRIEELGRARDLIRVAALLG
                     RETPVTILRAVMQIGDEEFLSRLRKLELADLVHLRDVSGEPCCVFAHILIQEAAKDAI
                     PAETSGALHASIVQVLDRDFSDRVLRNPERFAHLYATAGACERALALFELAANRAARD
                     SAHDEAAAHLQAALSLLQSRTDATDVAIERRLRGALGPCLMAIEGWSAGAVAENLNRS
                     RSLGDEASELRELWGTWAHGLVTHDVATVRNALAAVNRATPGAEQRFAVLSMEGVTAF
                     YRGQFASSRRYLEQAVAMLPPLEGDAASVTDERVSLANARSWGCEFVVAASMHLAWLE
                     ALSGRRERAEAVRANAEAILAHLSSDGELRETRHALYMQIHLGLTLRDYEYLGYSGLA
                     SGTGPLYRLFELAGGRFPYYRCVAQFGEARARAAGGDGSAIDDLLIVYERMKTISSRP
                     TGHVFLASILAEACIEAGRLSEAERLLDEAMQVADTEFGRFYAPEAYRLAAECALALG
                     ELRVARKRVRQARTACLTLDVPLESPVLHFERNLARTEASLVERVPLSPHSSDVSAPS
                     ALDDGHVADAKAQEPERRAALDEP"
     gene            complement(2568..>3490)
                     /locus_tag="VFQ35_14885"
     CDS             complement(2568..>3490)
                     /locus_tag="VFQ35_14885"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9931985.1"
                     /translation="PVSDPMGSLQNTKSGSRVRLAARCLVGRGSSCHLVLDDEDVSRE
                     QAVIFYHETGWRIRDLSSTNGTFVQGDRIRDTVALELGAAIRFGNSASVWVLSEDGPP
                     FARAISSRGEVIVATGSGLIIPGSEGVEAYVSQHDGEWLLERHGEVRPLCDMEEATFG
                     NATWRFELTTGDQAPQASTAIATGKEPQLIFNVSPDEEHVEIVFQSGVERHVLAHRSH
                     SYLLLLLARVRLDDQQSETTTSAEQGWIETKELASMMRSSNEQVNLWIWRAREQFERV
                     SADLASRLVERRPTLGLLRLGVGNVSIVKR"
BASE COUNT          559 a         1124 c         1162 g          645 t
ORIGIN      
        1 tcgcgagcga ggacgctccc ggtgagcctc tcggtactcc gacgtacgtc gtaacggaga
       61 tgctttcgca cgacgcgggg atcgagcaag tgtcgaccac gcccctgcga gcgcgcgcac
      121 gacagctcga gcagctcgag cgcgcatttc aggacgcgca gcgcggggcg gtagtgactc
      181 ggctcatcgt cggtatcgcc ggtgccggca agtcgcgcat gcttcacgag tggcgtcgcc
      241 gcagctggca agcgaccgac cttcttctga ccgcgtacgc cacggtccac gagcaccact
      301 caccgttcgc tccgctgctg cgcatgtggc ggagctggtt cgggtttccg ttggacgatc
      361 ctccgcaggc gatccgcgag cgcctgcaaa ccgtgctgga acaggccgaa ctccccgtcg
      421 aaaagttcgc cgcgcctttg gcgtatgcgt tcggcgcggg cgacgacatc gtttcgaagc
      481 aaccggccga gcgtcgtcgt cacctcgtca tccaggcgtt cgtcgagctc acgatcgcgc
      541 tcgccgagaa gcgtctcgtc gttctcgcgt tcgaggactt gcacttcgtc gaccgttcga
      601 cgctcgacgt gctgcggcgg ctcgtagaaa gcgcgcgacc cgctcgtctc ttgctcgtcc
      661 tgaccgcgcg acccgagttc gtcgtgggat ggccgctcgc caatcgcatc tcgcgcatcg
      721 acgtgacgcc gctcggccaa tccgacgcac tgcaattggt ggagagcatg tcgcagcgct
      781 cttcgctgcc gacggctttg gtccgccgca tcgtcgattt gagcgacgga gtcccactcg
      841 tgctcgagga gctggttcgg gcggctctgt ccgattggga gcgcgacgga gaagtcgctg
      901 atctgatgtc gcgcccaccg acatcctttg cggactcggt ggcgcgccgt atcgaagagc
      961 tcggtcgggc gcgcgatctg attcgggttg cggcgttgct cggacgagag actccggtca
     1021 cgattttgcg cgccgtgatg cagatcggcg acgaggaatt tctcagtagg ctgcgaaaac
     1081 tcgagctcgc cgacctggtc cacctgcgcg acgtgagtgg tgagccgtgc tgcgtgttcg
     1141 cccacatcct gattcaagaa gcggccaagg atgcgatccc ggccgagacg tcgggggcgc
     1201 tgcacgcctc gatcgtgcaa gtgctcgatc gggatttcag cgaccgggtc ttgcgcaatc
     1261 ccgagcgctt cgcgcacttg tacgcgaccg ccggcgcttg cgaacgcgct ctcgcgctat
     1321 tcgagctcgc cgcgaatcgc gcggcgcggg actccgcgca cgacgaggcg gcggcgcact
     1381 tgcaagccgc gctctctctg ctgcaatcga gaacggacgc gaccgacgtc gcgatcgagc
     1441 gccgtctgcg cggggcgctc gggccgtgcc tgatggcgat cgaggggtgg agcgccggag
     1501 cggtggcgga gaacctcaac cgcagccgtt cgctcggcga cgaagcgagc gagctgcgcg
     1561 agctgtgggg cacctgggcc catggcctcg ttacgcacga cgtggcgacg gtgcgcaacg
     1621 cgctcgcggc cgtgaatcga gcaacgcccg gagccgagca gcgcttcgcc gtgctctcga
     1681 tggagggtgt caccgccttc taccgcggcc aatttgcgag ctcccggcgc tatctggagc
     1741 aagcggtcgc gatgctccct ccgctcgaag gcgatgccgc gtcggtcacc gacgagcggg
     1801 tttcgctcgc gaatgctcga agttggggat gtgagttcgt cgtggccgct tcgatgcacc
     1861 tcgcttggct ggaggcgttg agcggtcgtc gcgaacgtgc cgaggcggtg cgcgcgaacg
     1921 cagaggcgat actggcgcac ctgtcgagtg acggagagtt gcgcgagacc cgacacgcgc
     1981 tctacatgca aattcacttg ggcctcaccc tgcgagatta cgagtacttg ggctactcgg
     2041 ggctggcgag cggaaccggc cctctgtacc ggctgttcga gctagcgggg ggacggtttc
     2101 cctattaccg atgcgtggcc caattcggcg aggcgcgcgc tcgagccgcg ggcggagatg
     2161 gcagcgcgat cgacgatctc ttgatcgtct acgagcgcat gaagacgata tcgagtcgtc
     2221 ccaccggtca cgtgtttctc gccagcattt tggcagaggc gtgcatcgaa gcggggcggt
     2281 tgtccgaagc ggagcgcctc ctcgatgaag cgatgcaggt tgcggacacc gagttcggac
     2341 gcttctacgc tcccgaggcg taccggttgg cggcggagtg tgcgctcgcg ctcggagagt
     2401 tacgagtggc gcgcaagcgg gttcggcaag cgcgcacggc gtgtctgacc ctcgatgtcc
     2461 cgctcgagag cccggtgctc catttcgaac gaaacttggc gcggacggaa gcctcgctcg
     2521 tcgagcgggt gccgctctcg ccgcattcga gcgacgtatc cgcgccgtca gcgcttgacg
     2581 atggacacgt tgccgacgcc aaggcgcaag agcccgagcg tcgggcggcg ctcgacgagc
     2641 cgtgacgcca gatcggcgct gacccgctcg aactgctccc gcgccctcca gatccaaagg
     2701 ttaacttgct cgttgctcga gcgcatcatg cttgcaagct ccttggtctc gatccagccc
     2761 tgctcggcgg acgtcgtggt ctcggattgc tgatcgtcga gtctgacacg ggcgagcagc
     2821 aggagtagat agctgtggct ccgatgcgcc agcacgtgac gttcgacccc gctttggaag
     2881 acgatttcca cgtgctcttc gtccgggctc acgttgaaga tgagctgtgg ctctttaccg
     2941 gttgcgattg cggtcgaagc ttgaggcgct tgatcgcccg tcgtcagttc gaagcgccag
     3001 gtcgcgttgc cgaaagtcgc ttcttccata tcgcacagcg gccgcacttc tccgtggcgt
     3061 tcgagcagcc actcaccatc gtgctgcgat acgtacgctt cgacgccctc gctgcctggt
     3121 atgatgagcc cgctcccagt cgccacgatg acttcgcctc ggctcgaaat ggcccgcgcg
     3181 aagggcggtc cgtcttcgct cagcacccac acgctcgcgg agttgccgaa gcggatggcc
     3241 gcgcccagct cgagcgcgac ggtgtcgcga attcggtcgc cctggacgaa agtcccgttc
     3301 gtggaagaaa gatcgcgaat gcgccaaccg gtctcatggt agaaaatgac cgcctgctct
     3361 cgcgaaacgt cctcgtcgtc caagactagg tggcagctac tgccacggcc gaccaggcag
     3421 cgagcagcca aacggaccct gcttcccgat ttcgtattct gaaggctgcc catgggatca
     3481 gagacggggt
//
LOCUS       DASTKS010000720        10378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1901923, whole genome shotgun sequence.
ACCESSION   DASTKS010000720 DASTKS010000000
VERSION     DASTKS010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10378
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901923"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1792)
                     /locus_tag="VFQ35_14890"
     CDS             complement(188..1792)
                     /locus_tag="VFQ35_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931986.1"
                     /translation="MPSIPQRRSLVPSSAGQDAYSDLLSGTRRLMREQRADREIWFVG
                     LNLEGKDELLFEFEVLLKATACFSNPRNHPGPPRRAAVVAQDFRGPLWVFREGLSRAV
                     SLARSLLGSRDRAFVFHRYLETVLPEDQARTRLLTEGTNQTTPEESLVTLRHGLTAAL
                     EVTEGTLRLQRVPFRLFHAVLATAYREVDRNNYFNPLSALEFRPEFDRIKSAQVLELI
                     QSVPGVEAHRLVSLTFLSLFRMLRYLQLLTRIANDPAQRRQGIGSAYLILSVLRSDAR
                     ALSDYLRQRSGSLLAESFERDVLRVPAGELRTRATVLRAAGHRLIGIKSALEGIAGNL
                     RLEMRRTLQHDIPEPHANLTPADFQRALSTATTNLRPALQNAILFLGKALGVSLEEGG
                     VFDHQAARRETSERLRRDVWMFGQIVRAFSTKAQHSPNEDRWAPVYNFQYVREFLAYF
                     RAMGYPLLRAADYPRFDAFMAAMTGLEDTDLVDPSRLEAAIDECMAFHGFLSQLFEDI
                     SRRDELVGVPFDKRAAAAALRLYLGD"
     gene            complement(1928..2608)
                     /locus_tag="VFQ35_14895"
     CDS             complement(1928..2608)
                     /locus_tag="VFQ35_14895"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9931987.1"
                     /translation="MALLSIRYKKSLAYCVLSLPLVVGCTITTGPAQPGTQTPAAAPP
                     PPPPPAATPAQPQGNNDDNWEGEDEATRRPRTHDSIDRRRARPQLGGAGEPARTPPTP
                     ATPATPATPAQPARSSHAAVGLDPTGETRTTEVIASVIRNNRQPFRDCYERSAAKDPN
                     LQGTLTLHFVLDPEGRVKLAELNDSRSTIKDQNVVNCAIGVLKGLRFPASSRGMESVA
                     NYPFDFKR"
     gene            complement(2813..3421)
                     /locus_tag="VFQ35_14900"
     CDS             complement(2813..3421)
                     /locus_tag="VFQ35_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9931988.1"
                     /translation="MDSEASSEARCKREVLTLPVLLLNRHFSPVSVATVRRAFVLLYG
                     GAARAVDAEGETYDFSAWRRVPVRSLDDGLPIIGGHIRAPRVLHLLRYDRAPKVSVRL
                     TRRNLMIRDQFQCQYCGRRPNTRELNLDHVVPRSRGGADSWENLVVSCRTCNLRKGRR
                     TPLEAGMTLLTVPHHPRWSTATQIRLVMREPFAEWQPFLQTG"
     gene            3945..5420
                     /locus_tag="VFQ35_14905"
     CDS             3945..5420
                     /locus_tag="VFQ35_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931989.1"
                     /translation="MIRQSLASLGATLFLVSSLSGCAGGAGVGEAAWPPLGKKWYERA
                     DASFQRGDMEDAREAIDNALKVVPDRPEARVLSAKVALASLDYDRVQAALRGIETSEA
                     RSLRGRAYWYAGDVEHAADELEKLLADPEVRDPWATEIAKLARLGAGRKPFDVSGSMV
                     GFTEMPRAGTSALIVPLDINGEPALGLVATGKAEAVIDSSGGARASWVSLRFGERLDV
                     RDVPVLAEDLSGVSRQVNAPIKILLGVNLLRHLRPTIDFAGGQFVVRAFEPPPPPVAT
                     TVKLSYVRGGGMLVRGAFGADQNAQPCSLMIDTTMSFPVALSSDAWKKAGVSPASLQP
                     VPGASDLRQGFVPALRLGAFDIPRVPGVQGDSVVKEREERLGISFDGLIGSGLLAGFR
                     VTLVDGGRSMWLEDIPAEALNAPSLFANLPPIEEAPDAEVPEEAEKPGKGKPGAAPRS
                     PASPSGAAPTPKSPVSPSGAAPAPAPKSPTAPPGAPKGTKP"
     gene            5417..5911
                     /gene="coaD"
                     /locus_tag="VFQ35_14910"
     CDS             5417..5911
                     /gene="coaD"
                     /locus_tag="VFQ35_14910"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9931990.1"
                     /translation="MSADAELAIYAGSFDPPTYGHLDLVERASKLFPRVLVAVGHNSQ
                     RTALFTVEERLELLRGVCAPFTNVSVDSFQGLLVDYGKRVGARVIVRGLRAGTDFEYE
                     LQIAHANADLRPEIDTVFLPTRTNYGFISASLVREIAVNGGEVSRYAPPSVCAALKRK
                     FDRR"
     gene            complement(6036..7013)
                     /locus_tag="VFQ35_14915"
     CDS             complement(6036..7013)
                     /locus_tag="VFQ35_14915"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9931991.1"
                     /translation="MSADPTSSEATEATRAPSQVVPLVLFLATVWSVFHAGALLAGQL
                     APSGPLVGLFDVRFLLTGASFALPLLAILVTHELGHYVAARWHGLPASLPYFIPLPLL
                     GLFGTLGALIGMHDEVRSRNALLDLGASGPLAGLLIAVPTLVWGLAHSHFAPQLSTGY
                     TQEGASLFYVFLKRVVAGPTPPGYDIMLHPTAFAGWAGLWLTMLNLFWWGQLDGGHVA
                     FALLGDRHHRLARWSRRGLLVLFGYNIVRFVVPVLLGKSHSGLGQALSNSSFWLVWFV
                     VLHVVERAAGEADHPPFEPGPLSPFRKVVGWACMALFALLFMPTPLAQY"
     gene            complement(7003..7659)
                     /locus_tag="VFQ35_14920"
     CDS             complement(7003..7659)
                     /locus_tag="VFQ35_14920"
                     /inference="COORDINATES: protein motif:HMM:NF017952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HET9931992.1"
                     /translation="MALGFGKIVPPGVARSAEPITLPAVSPAPGAHIVPPEVVAASAR
                     ARAILERAEAEAAALRARADADIAGLRAQAIEDARAHAVSRLAAETLALAQRDADADA
                     RALERSVELARLLAERLLDEELALKPSRVEAIARRALTEASGARRIRIVCHPEDAPFL
                     QAALEAGRFSHVSEIRGDSARRRGNLRFETEIGSLDADIAPQLERLADRLHQALVHER
                     "
     gene            complement(7659..7886)
                     /locus_tag="VFQ35_14925"
     CDS             complement(7659..7886)
                     /locus_tag="VFQ35_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931993.1"
                     /translation="MDSVVNSAAFQRAFVAVSYFLNRRGEELLAPLPQKTQAAVALAK
                     SLGHEAREQRAQALAGEIARIVAALESARAF"
     gene            complement(7895..9079)
                     /locus_tag="VFQ35_14930"
     CDS             complement(7895..9079)
                     /locus_tag="VFQ35_14930"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9931994.1"
                     /translation="MSASTVYVVSAARTPIGAYLGSLSALPAPKLGAVAIKGALERAR
                     VAPELVEQVFMGNVLSAGIGQAPARQAAIFAGLPQTVPATTVSKVCGSGLEAVVLGAR
                     TILTGDASIVAVGGMESMSNVPYYLREARTGYRMGDGKLVDGMIFDGLWDPYNDFHMG
                     QAGELCSRELDIGRAAQDDFARESYRRAREAQAKHAFADEIVKVSVPQRKGAALEVSV
                     DEEPGRGDPAKFSDLKPAFAKDGTITAANASSISDGAAALVLASEAAVKAHGLSPLAR
                     IVGFGGAAQAPEWFTTAPARAVERTLQKLQLQADAIDLWEINEAFACVTIACSRLIGI
                     SPEKVNVRGGAVALGHPIGATGARILTTLIHSLREQGKQRGLATLCIGGGEAIALVVE
                     RV"
     gene            complement(9108..10082)
                     /locus_tag="VFQ35_14935"
     CDS             complement(9108..10082)
                     /locus_tag="VFQ35_14935"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9931995.1"
                     /translation="MTLPQEVFEQALRHPLAHTEFSFGARYEGKVRDNYTTPDGRRIL
                     IVSDRVSTFDRIVSTLPLKGQLLNHVSNWWFDKTRDVCPNHVLAAPDPNVLVAVECTP
                     LPVEMVVRAYLTGTTSTSIWVHYSRGERVFCGHQLPNDLRQHQALPEPILTPSTKAEK
                     GDHDVSASRDEILRISKMAPEDFDRAAELAMSLFRRGQEICRARGLILVDTKYEFGRA
                     PDGRIVVIDEIHTPDSSRFWYAESYAERFARGEAPESLDKEFLRRWLVERGFSGDGPI
                     PEIPDAIRIEAARRYLTTIELITGESFRPNLEEPIARIQRNVSAYLAS"
     gene            complement(10110..>10378)
                     /locus_tag="VFQ35_14940"
     CDS             complement(10110..>10378)
                     /locus_tag="VFQ35_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931996.1"
                     /translation="DQLSSRSSIRSPTRLAIQGEPRIVKPVRDVPLRAAAHESDAPIG
                     VIEPGAETYVLDVVAGWASVMPKALNVVPGPDRQFWVKTSDLGI"
BASE COUNT         1739 a         3259 c         3632 g         1748 t
ORIGIN      
        1 ctctttccgg gtgtcctgag cgatcgatgt ctccgggctg actctcgatc gatgtccaag
       61 gcgtctactc ccgcctgcgc agggtcgctg tgttgaagcg ttttgagctt ctcggacgct
      121 caatcgtcgc gtgcgcttct cgctcgcgtc tcgtggctct cgcgctgagc agaaacttgg
      181 gacaccccta gtcgccgagg tagagccgta acgccgcagc ggccgcgcgt ttgtcgaagg
      241 ggactcctac gagctcgtca cggcgcgaaa tgtcctcgaa gagctgcgac aagaagccgt
      301 gaaatgccat gcattcgtcg atggcggctt cgaggcgcga agggtcgacc aagtcggtgt
      361 cttcgaggcc cgtcatggcc gccatgaaag cgtcgaagcg cgggtagtcg gcggcgcgga
      421 gcagcgggta tcccatcgcg cggaagtagg cgaggaactc gcgcacgtat tggaaattgt
      481 agacgggcgc ccagcgatct tcgttgggcg agtgttgggc cttagtcgag aaggcgcgca
      541 cgatttgccc gaacatccac acgtcgcggc ggaggcgctc actcgtttcg cggcgcgctg
      601 cttgatggtc gaatacgccg ccttcctcca agctgacgcc gagcgctttg ccgaggaaca
      661 gaatcgcatt ctgtagggcc gggcgaaggt tcgtggtggc ggtggagagc gcgcgttgaa
      721 aatcggctgg ggtgaggttc gcgtgaggct cggggatgtc gtgctggagc gtgcggcgca
      781 tttcgagccg gagattcccc gcgatgccct cgagcgcgct cttgatgccg atcaagcggt
      841 ggccggcggc cctcagcacg gtcgcgcgag tgcgaagctc gccggcgggt acgcgcagca
      901 catcgcgttc gaagctctcc gccaacagcg agcccgagcg ctgacgcagg tagtcgctga
      961 gagcgcgcgc gtcggagcgc aggacgctga ggatgagata ggcgctgccg atgccctgac
     1021 ggcgctgcgc gggatcgttc gcgatgcgag tcagaagctg caagtagcgc agcatccgaa
     1081 agagcgacaa gaacgtgagc gacacgagcc ggtgcgcttc gacgcccggc acgctctgaa
     1141 tcagctccag aacttgcgcg ctcttgatac ggtcgaactc ggggcgaaac tcgagcgcgc
     1201 tcagcggatt gaagtagttg ttccgatcga cctcgcgata tgccgtcgcg agcacggcgt
     1261 ggaacagccg aaaagggacg cgctggagcc gcagcgtccc ctcggtcacc tcgagcgcgg
     1321 cggttagccc gtggcgtaaa gtgacgaggc tctcctcggg cgtcgtctgg ttcgttccct
     1381 cggtgagcag ccgggtccga gcttgatctt cggggaggac cgtctcgagg tagcggtgga
     1441 aaacgaacgc gcggtctcgt gaaccgagca ggctccgcgc gagcgaaacg gcccgtgaca
     1501 gcccctccct aaaaacccag agcggcccgc ggaagtcttg cgcgaccacc gccgcccgac
     1561 gcggtggccc cgggtgattg cgcgggttgg agaagcacgc cgtggccttc agcagcacct
     1621 cgaactcgaa cagaagttcg tcttttcctt cgaggttgag cccgacgaac cagatttctc
     1681 ggtcggcgcg ttgttcgcgc atcagccggc gcgtaccgct caaaagatcg gagtacgcgt
     1741 cttgaccggc gctgctgggg acgaggcttc tgcgctgcgg gatagacggc atgaggtttg
     1801 cgctgaacgg agcgcggcta tgcgccttgc atcatcggac tggcgcgaag agcgcgcctt
     1861 gcatcatcgg aggcgcgaag agcacgcctt gcatcatcgc aggcgcgaag agcgcgcccc
     1921 gtaggaccta gcgcttgaag tcgaacggat agttggcgac gctctccatc cctcgtgacg
     1981 aagcggggaa acgcagacct ttcagcacgc caatcgcgca atttacgacg ttttggtcct
     2041 tgatggtcga acgagagtcg ttgagctcgg cgagcttcac gcggccttcg ggatcgagga
     2101 cgaagtgcag ggtcaacgtg ccttggaggt tcggatcctt cgcggcgctg cgctcgtaac
     2161 agtcgcggaa gggctggcga ttgttgcgga tcacgctggc gatgacctcg gtggtgcgcg
     2221 tttcgccagt gggatcgagc cccacggcgg cgtgactact gcgggcgggc tgggcaggag
     2281 tggcgggtgt cgcaggggtg gcgggggttg ggggcgtgcg cgccggctcg cctgcgccgc
     2341 cgagctgagg gcgagcccgt cgccggtcga tgctgtcgtg cgtgcgcggg cgtcgagtcg
     2401 cttcgtcctc gccctcccag ttgtcgtcgt tgttgccttg gggctgagca ggggtcgcag
     2461 cgggcggcgg agggggtggc ggcgccgcgg ctggagtttg cgtccctggt tgcgcgggac
     2521 ccgtcgtgat ggtgcagccc acgacgagag gcagggacaa cacacagtag gctaaggact
     2581 tcttgtagcg gatcgataga agcgccatgc gactcatctt agtcagactt tcgggggctc
     2641 gtctttagtc acgagcggcg cgaaaaagcg cagcaacggg aggcttcaac cggacggtgg
     2701 agttgcgtcg cgcattcttt ggatgcgtgg ccagatgccc aacaccgacg gcggcttggt
     2761 tatctgcaca gcagagccgg tcgagagggc cgcaagagcg atggatccga gctcagccgg
     2821 tttgcaagaa gggctgccac tcggcgaacg gctcgcgcat gacgagccgg atttgcgtgg
     2881 cggtcgacca gcgcgggtga tgcggcacgg tgagtagcgt catgcccgct tcgagcggcg
     2941 tgcgtcggcc ctttcgcagg ttgcacgtgc gacacgagac gacgaggttt tcccagctat
     3001 cggcgccgcc gcgcgaacgt ggcacgacgt gatccaggtt gagttcgcgc gtgttcgggc
     3061 ggcgaccgca gtactggcac tggaactgat cgcgaatcat cagattccgc cgcgtcagcc
     3121 gcacggagac tttgggcgcg cgatcgtaac gcaataagtg caacacccgc ggtgctcgaa
     3181 tgtgcccgcc gatgatcggc aagccgtcat cgagcgagcg caccggaacg cgccgccacg
     3241 cactgaaatc gtaagtctcg ccttctgcgt ccacggcgcg ggcggcgcca ccgtagagca
     3301 gcacgaacgc acgacggacg gtcgccacgc tcaccggcga gaaatgccgg ttcagcagta
     3361 ggaccggcag cgtcagcacc tcgcgtttgc agcgagcctc gctggatgcc tccgagtcca
     3421 tagactccac gggttttcag catacttcag attccatact ttggcgaaag gcacccacgc
     3481 cgcgacggac gcgaaggatc ttctggagtt gtgcgtgttt gtgtccgtca ccgctcgcag
     3541 ccggggctcg cgacggcgtg ccgggatcca ggttttttct gcattccggt tacgacatcc
     3601 gcagagcggc gcattcctgc aggggcgcaa cgcggctcag gtgcgaccgc cttcgagtgc
     3661 tcgctggacg cggaaagtcg gcgcccgcgg aaccgctcga gcgagccgct caggggaact
     3721 ttctggggct ttggggcccc aagcgcgtct aggaggcggc cgcgcccctc gacgggacga
     3781 ggattgttac tggcctgcct cgctgaccct tttcagcgaa ccgaatgggc gagtgcacga
     3841 aaccgcgcgc aaagtcgtcg cccacactcg accccgctgc agcaatacgc taatcccccg
     3901 tcgccctggc gctcccgccg tcttccacaa aacccatggc ctcaatgatt cgccaaagcc
     3961 tcgcgtccct cggcgccact ctgtttctgg tttcctcact gagcggttgc gctggtggag
     4021 cgggtgtggg tgaagcggcg tggccgcctc tcggcaagaa atggtacgag cgcgcggacg
     4081 cgagctttca gcgcggcgat atggaagatg cgcgcgaagc catcgacaac gctctgaaag
     4141 tggtccccga tcgacccgag gcgcgcgtgc tgtcggccaa ggtggcgctc gcgagcctcg
     4201 actatgatcg cgtgcaggcg gcgctgcgcg gtatcgagac gagcgaggcg cgtagcctgc
     4261 gcggacgcgc ttattggtac gcgggcgacg tcgaacacgc cgcggacgaa ctcgagaagt
     4321 tgctcgccga ccccgaggtg cgcgacccgt gggcgaccga gattgcaaag cttgctcgct
     4381 tgggtgccgg ccgaaagccg ttcgacgtga gcggcagcat ggttggcttc acggagatgc
     4441 cgcgcgcggg tacgagcgcg ctcatcgttc cgctggatat caacggcgaa ccagcgctcg
     4501 ggctcgtcgc gaccggtaag gcagaggccg tcatcgactc gagtggcggc gcgcgggcga
     4561 gctgggtttc gctgcgcttc ggcgagcgcc tcgacgtgcg cgacgtgccc gttcttgctg
     4621 aagacttgtc gggcgtttcg cgccaagtga acgctccaat caagatcctt ttgggagtga
     4681 atctgctccg ccacttgcgg cccaccatcg acttcgcggg tggccaattc gtggtgcgcg
     4741 ccttcgagcc gccgccccca cccgtggcta ccacggtgaa gctcagctac gtgcgcggcg
     4801 gcggcatgct ggtgcgcggc gcattcggag ctgaccaaaa tgcgcagccc tgttcgctga
     4861 tgatcgacac gacgatgagc tttccggtcg cgctcagcag tgacgcttgg aagaaggcgg
     4921 gcgtgagccc ggcgagtctc caaccggtgc cgggagcgag cgacctgcga cagggtttcg
     4981 tgccggcgct gcgtttgggc gcgtttgaca tccctcgcgt gcccggggtg caaggcgaca
     5041 gcgtcgtgaa ggagcgtgag gagcggctcg ggatcagctt cgacggcttg atcggctcgg
     5101 gattgttggc tgggttccgc gtcacgttgg tcgacggtgg acgctcgatg tggctcgagg
     5161 acatccccgc ggaggcgctc aacgcgccct cgctgttcgc gaaccttcct ccgatcgagg
     5221 aggcgccgga cgccgaggtg ccagaggagg ccgaaaagcc tggaaaaggt aagcccggtg
     5281 ccgcgccgag gtcaccggca tcgccttcgg gggccgcacc cacgccgaag tcgccggtat
     5341 cgccttcggg ggccgcgcct gcgcccgcac cgaaatctcc cactgcgccg cccggcgcgc
     5401 ccaagggaac caagccgtga gcgccgacgc cgagctcgcg atttacgcgg ggagcttcga
     5461 cccgccgact tacggtcacc tggacctggt cgagcgcgct tcgaagttgt tcccgcgtgt
     5521 gctcgtcgcg gttgggcaca actcacagcg cacggcgctc tttaccgtcg aggagcgcct
     5581 cgagcttttg cgcggcgtgt gcgcgccgtt cacgaacgtc tcggtggact cgtttcaggg
     5641 gttgctcgtc gattacggca agcgcgtcgg cgctcgcgtc atcgtgcgcg gtttgcgcgc
     5701 ggggaccgac ttcgagtacg agctgcaaat cgcgcacgcg aacgccgatt tgcgaccgga
     5761 aatcgacact gttttcctgc cgacgcgcac gaactacggt ttcatttcgg cgtcgctcgt
     5821 ccgcgaaatc gcggtcaacg ggggagaagt gagccgttac gcgccgccct cggtttgcgc
     5881 ggcgctgaag cgcaagttcg accggcgctg agtcggcgct gaggcgcaag ttcgaccgcc
     5941 gctgaaacgt cgcgagcgac ggcgttgccg cgagtccgtg cgcggctcta cgccgaactg
     6001 cagcaaagcc gcgcgacagc gagcccgctt ggctttcagt actgcgcgag gggtgtcggc
     6061 atgaacaaga gcgcgaagag cgccatgcag gcccaaccga cgactttgcg gaatgggctc
     6121 aacggccccg gttcgaaggg cggatggtcc gcctctccgg cggcgcgctc gacgacgtgc
     6181 agcacgacga accaaaccag ccagaacgac gagttcgaca gggcttggcc caggcccgaa
     6241 tggctcttgc cgagcagcac cggaacgacg aagcggacga tgttgtatcc gaaaaggact
     6301 agcagaccgc ggcgagacca gcgcgcgaga cggtgatggc ggtcacccaa gagagcgaac
     6361 gcgacgtgac ccccgtcgag ctgaccccac cagaaaaggt tcaacatcgt gagccacaac
     6421 cctgcccagc ccgcgaacgc ggtgggatgc agcatgatgt cgtaacccgg cggggtaggg
     6481 ccggcgacga ctcgcttgag gaacacgtaa aagaggctcg ccccctcctg cgtgtagcca
     6541 gtggaaagct gcggagcaaa gtgcgagtga gcgagccccc agacgagcgt cgggaccgcg
     6601 atcagcaaac ccgcgagtgg acctgacgcg cccagatcga gcagcgcgtt gcgagagcgg
     6661 acctcgtcgt gcatgccgat gagcgctccg agagtgccga acaacccgag cagcggaaga
     6721 gggatgaaat agggcagcga cgcgggcagg ccgtgccagc gcgcagcgac gtagtgaccg
     6781 agctcgtgtg tcaccaggat cgcgaggagc ggcagcgcga acgatgcgcc cgtgaggagg
     6841 aaacgcacgt cgaagagccc gacgagcggc ccgctcggcg cgagttgccc tgcgagcagc
     6901 gcgccggcgt gaaacaccga ccacaccgtt gcgagaaaca ggacgagcgg gacgacttgc
     6961 gacggcgcgc gggtcgcttc ggtcgcttcg ctcgaggttg ggtcagcgct catgcacgag
     7021 cgcttggtgt agccgatccg cgagacgctc gagctgaggc gcaatgtcgg catcgagcga
     7081 cccgatttcg gtctcgaagc gcaagttgcc gcggcggcgc gccgaatccc ccctgatttc
     7141 gctgacgtgg ctgaaccggc cggcctcgag cgccgcttgc aagaacggcg cgtcttcggg
     7201 gtggcagacg atgcgaattc ggcgcgcgcc gctcgcttcg gtgagggccc gtcgtgcgat
     7261 ggcttcgact cggctgggct ttagcgcgag ctcctcgtcg agcagtcgct cagcgagcag
     7321 tcgcgcgagc tccacgctgc gttcgagcgc tcgtgcgtcg gcgtcggcgt cccgctgagc
     7381 gagcgccaaa gtctcggcgg caaggcggct taccgcgtgc gcccgcgcgt cttcgatggc
     7441 ctgggcgcgg aggcccgcga tgtcggcgtc ggcgcgggcc cggagcgcgg cggcctcggc
     7501 ctcggcgcgc tcgaggatgg cacgggcgcg agcgctcgcc gcgacgacct cgggcggtac
     7561 gatgtgcgcg ccgggagcgg gcgagacggc tggaagcgtg atcggctccg cgcttcgggc
     7621 gacgccgggc ggtacgatct ttccgaaccc caaggccact agaatgccct cgccgactcg
     7681 agcgcggcga cgatgcgcgc gatttcaccg gcgagcgctt gcgcccgctg ctctcgcgcc
     7741 tcgtgaccga ggctcttggc gagcgcgacg gcggcctgcg tcttctgggg caacggcgcg
     7801 aggagctcct ccccgcggcg attcagaaaa tagctgacgg cgacgaaggc ccgctggaac
     7861 gcggcgctgt tcacgaccga gtccattcgc accctcagac gcgctcgact acgagcgcga
     7921 tggcctcgcc gccgccgatg cacagggtgg cgagccctcg ttgtttacct tgctcgcgca
     7981 acgaatgaat caacgtcgtg agaatgcgtg cgcccgtcgc accgattggg tgcccgagcg
     8041 cgaccgctcc gccgcgcacg ttgacctttt ctggcgaaat accgatgagg cgtgagcacg
     8101 cgatggtgac acaggcgaac gcctcgttga tttcccagag gtcgatcgcg tcggcttgga
     8161 gttgaagctt ttgcagcgtg cgctcgacgg cgcgcgcggg ggccgtggtg aaccattccg
     8221 gggcttgggc cgcgccgccg aacccgacga tgcgcgcgag cggcgacaag ccgtgcgctt
     8281 tgacggcggc ttcgctcgcg agaacgagcg cggccgcgcc atcgctgatg ctcgaggcgt
     8341 tcgcggccgt gatggtgccg tccttcgcaa aagcaggttt caaatcgctg aacttggccg
     8401 ggtcgccgcg cccgggttct tcgtcgaccg agacctcgag cgccgcgccc ttacgctgcg
     8461 ggacgctgac tttcacgatt tcgtcggcga aagcgtgctt cgcctgagct tcgcgcgcgc
     8521 ggcggtagct ctcgcgagcg aagtcgtctt gcgcggcgcg accgatgtcg agctcgcgcg
     8581 aacagagctc accggcctgc cccatgtgaa agtcgttgta cggatcccag agcccatcga
     8641 agatcattcc gtcgacgagc ttgccgtcgc ccatgcggta gccggtgcgc gcttcgcgca
     8701 ggtagtacgg cacgttggac atcgattcca tgccgccgac cgcgacgatt gaagcgtcac
     8761 cggtgaggat cgtgcgcgcg ccgaggacga ccgcttcgag cccggagccg cacaccttgc
     8821 tcacggtcgt ggctggcacc gtctgcggca gccccgcaaa aatggcggct tggcgcgcgg
     8881 gcgcttgccc gatgccggcg gagagcacgt tgcccatgaa cacctgctct acgagctccg
     8941 gagcgacgcg ggcgcgttcg agcgcgccct tgatggcgac cgcgccgagc tttggggctg
     9001 gcaacgccga gagggatccg aggtaggcgc cgataggagt tcgcgccgcg ctgactacgt
     9061 agacggtgct cgctgacatc ccagcgagca tagcagctcc ggcggactca agacgccagg
     9121 tacgcgctca cgttgcgctg gattcgcgca atcggctcct cgaggttcgg ccgaaatgac
     9181 tcgccggtga tgagctcgat ggtggtgagg tagcgccgcg ccgcttcgat gcggatggcg
     9241 tcgggaattt ccgggatggg gccgtcgccg ctgaacccgc gctcgaccag ccaacgccgc
     9301 aaaaattcct tgtccaggct ctcgggtgct tcaccccgcg cgaagcgctc ggcgtagctt
     9361 tcggcgtacc agaagcgaga cgaatccggc gtgtggatct cgtcgatgac gacgatgcgg
     9421 ccgtcgggag cgcgcccgaa ctcgtacttg gtgtcgacga ggatgagccc gcgcgctcgg
     9481 caaatctcct ggccgcggcg gaacagcgac atcgcgagct cggcggcccg atcgaagtct
     9541 tcgggagcca tcttcgagat gcgcaggatt tcgtcccgcg aagcggagac gtcgtggtcg
     9601 cccttctccg ctttggtact cggcgtgagg atgggctccg gcagcgcttg gtgctgacgc
     9661 agatcgttcg gcagttggtg gccgcagaag acgcgctcgc cgcgcgaata atgcacccaa
     9721 atgctcgtcg acgtcgtccc ggtgaggtag gcgcggacca ccatttcgac tggtaacggc
     9781 gtgcactcga ccgccacgag cacgttcgga tccggcgcgg cgaggacgtg gttcgggcac
     9841 acgtcgcgcg tcttgtcgaa ccaccagttc gagacgtggt tcaggagctg tcccttcagc
     9901 ggcagcgtgc tcacgatgcg gtcgaaggtg ctgacgcgat cgctcacgat gaggatgcgc
     9961 cgcccatcgg gcgtcgtgta gttgtcgcgc actttgcctt cgtaacgcgc gccgaaggag
    10021 aactcggtgt gcgcgagagg atggcggagg gcctgctcga atacttcctg cgggagcgtc
    10081 acgcggcgaa cgctacacca tggtccgctt caaatcccga gatcgctggt ttttacccag
    10141 aattgccgat ccgggccggg gaccacgttg agagctttgg gcatgacgct ggcccaaccc
    10201 gcgacgacgt cgagcacgta ggtttcggcg cccggctcga tgacgccgat cggcgcgtcc
    10261 gactcgtgcg ctgccgctcg cagcggaacg tcgcgcaccg gtttgacgat acggggttcg
    10321 ccctgaatcg cgagccgcgt ggggctgcgg atcgagctgc ggctgctcag ctgatcgg
//
LOCUS       DASTKS010000721         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_784049, whole genome shotgun sequence.
ACCESSION   DASTKS010000721 DASTKS010000000
VERSION     DASTKS010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784049"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1755)
                     /locus_tag="VFQ35_14945"
     CDS             complement(85..1755)
                     /locus_tag="VFQ35_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931997.1"
                     /translation="MRFTAVMLLCAASLATACAPTKAAMKAPPPKSHFGALGRTLTTT
                     PLLGPNAPNTSVVAVDAGVAGDRVSGLVEVPADECAVLIARAGPSVEDVDLLAYGEDG
                     STVGTDEGPDKTPALLVCPPHPTRIWVTARIAAGHGLVAVGVQRLSPTDAPHVAQSYG
                     ITDASNAASRIRNWPGLDEHLEAHRREVGGEWQDVRRVAIPLDARVPTRVSAQVDADR
                     CLDVFVAPSDDAGHIELVALDASGSIIARAASSGRERALVICSPLEAAIALELRPQSG
                     RGVGVLVLSRSRANTELDGDVDVTHVELFPSAELAAEVATVEARLSEQAGYAKGKQLA
                     SGTLEVGRRNSLNLNLPKGCVRLDVVGGKPLRGLEAVAWSSQGELVSRGRGGGTALLF
                     VCGMAGSVRLDLEATLRPGPYAVVMHTEAEVPSSLLAAPLAAGRLLGHLVTHGVLRRP
                     GEVVQAQEVQLSDTGIRSLDLTVPIGRCVDVALALGADTLGAEIRLVSVSRGVEITSG
                     RGAHAASARVCSLDASSANEHLKTRAELRVSAGKGIGLVATRMLTPAR"
     gene            complement(1752..>2373)
                     /locus_tag="VFQ35_14950"
     CDS             complement(1752..>2373)
                     /locus_tag="VFQ35_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931998.1"
                     /translation="DVLAEPTTGRAALGDLAVMPELPATASLVESERGDGLDAAFTLC
                     AGERASLAFRFAGAPPDSRMWLVASRVPLPAGLLDDWGNGGKARIAAVLYRHGVRVTS
                     APVDQALGVQGPTLMPISVVPGTCYVAAVAGVQGQVFGMALAARVAASEVQNHGGSSG
                     EGTLVSFCARGADTALVEVDSRGIGLTWLFALWEASRLPLGQEFPR"
BASE COUNT          395 a          839 c          801 g          338 t
ORIGIN      
        1 cgttggggcc gacgccacgc cgcgcttggt gccaacgcgc gatgtcctgc cgctcgcgcc
       61 ttcccccgag ccgcgttgag cggttcagcg cgctggcgtc agcatccgcg tcgcgacgag
      121 accgatgcct ttacccgccg agacgcggag ctcggcgcga gtcttcaagt gctcgttcgc
      181 gctgctcgcg tcgagcgaac acacccgagc gctcgcggcg tgagcgccgc ggccagaagt
      241 gatctcgacc cctcgactca cgctgacgag gcgaatctcg gccccgagcg tgtcggcgcc
      301 caacgcgagc gcgacgtcga cacagcgacc gattgggacc gtcaaatcca agctgcgaat
      361 accggtgtcg gagagttgca cctcctgagc ctgcacgacc tcgccgggtc ggcgcagcac
      421 gccgtgggtg acgaggtgac cgagtagccg ccccgccgcg agcggagccg cgagcagcga
      481 gctcggcact tccgcttccg tgtgcatcac caccgcgtag ggaccgggcc tgagtgtcgc
      541 ttcgagatcg agccgaaccg agcccgccat cccacagacg aagagcaacg ccgttcctcc
      601 gccccgacca cggctcacga gctcgccttg ggatgaccaa gcgacggctt cgaggccgcg
      661 caacggcttg ccgccaacta cgtcgagccg cacacaacct ttgggcaggt tcaagttgag
      721 gctattgcgg cgccccacct cgagcgtccc ggaggcgagc tgcttgcctt tcgcataccc
      781 ggcttgttcg ctcaggcgcg cctcaaccgt cgctacttcg gccgcgagct cggcgctcgg
      841 aaagagctcg acgtgagtga cgtcgacgtc accgtcgagc tcggtgttgg ctcgacttct
      901 cgaaaggacc aaaacgccga cgccgcgccc cgactgtggc cgcagctcga gcgcaatcgc
      961 cgcctcgagc ggagaacaga tgacgagcgc ccgctcgcgc ccggagctcg ctgcccgcgc
     1021 gatgatggag ccgctcgcgt cgagggcgac gagctcgatg tgaccggcgt cgtcggaagg
     1081 tgcgacgaag acgtcgaggc accgatccgc gtcgacttgc gcggagactc gagtgggcac
     1141 tcgggcgtcg agggggatgg ccactcgcct cacgtcttgc cattcgccgc ccacttcgcg
     1201 tcggtgcgcc tccaagtgtt cgtcgaggcc aggccaattg cggatgcgtg acgcggcgtt
     1261 gctcgcgtcg gtgatgccat acgactgtgc gacgtggggc gcgtcggtcg gcgagagacg
     1321 ctgcaccccc accgcgacga gcccgtggcc ggccgcgatg cgcgcggtca cccaaattcg
     1381 tgtggggtgg ggcggacaca cgaggagggc cggcgtttta tcggggcctt cgtcggtgcc
     1441 gaccgtactc ccgtcttcac cgtaggcgag cagatcgacg tcttcgacgc tgggcccggc
     1501 ccgcgcgatg agcaccgcgc attcgtcggc ggggacctcg acgagcccgg agacgcggtc
     1561 acccgcgacg cccgcgtcga cggcgacgac gctcgtgttc ggcgcgttcg gcccgagcaa
     1621 gggggtcgtc gtcaacgtgc gaccgagcgc cccgaaatgg ctcttcggcg gcggcgcctt
     1681 catggcggct ttggttggcg cgcaagcggt cgcaagcgac gccgcgcaga gcagcatgac
     1741 ggcggtgaac ctcatcgcgg aaactcctgc ccgagaggca aacgagaggc ctcccaaagc
     1801 gcgaacagcc aggtgaggcc gatgccccga gagtctacct cgacgagcgc cgtatccgcg
     1861 ccacgggcac aaaaggagac caaggtccct tcgcccgaag agcctccatg attttgcact
     1921 tcgctggcgg cgacgcgcgc ggcgagcgcc atgccgaaga cctgaccctg cacccccgcg
     1981 accgcggcca cgtaacaggt cccaggcacc accgatatcg gcatcagcgt ggggccttgc
     2041 acaccgagcg cttgatcgac gggcgcgctc gtcacgcgca ctccgtgccg gtacaaaaca
     2101 gcggcgattc gtgcttttcc accgttaccc cagtcgtcca gcaggcccgc ggggagcgga
     2161 acgcgtgaag caacgagcca catgcgcgaa tcagggggcg cgcccgcaaa acgaaaggcg
     2221 agcgacgcgc gttcgccagc gcagagcgtg aaggccgcgt ccaacccgtc gccgcgctcg
     2281 ctctcgacca ggctcgcggt cgcggggagc tccggcatca cggccagatc accgagcgcg
     2341 gcgcggccag tcgtgggttc cgcgagcacg tcg
//
LOCUS       DASTKS010000722         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_561630, whole genome shotgun sequence.
ACCESSION   DASTKS010000722 DASTKS010000000
VERSION     DASTKS010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561630"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFQ35_14955"
     CDS             <1..209
                     /locus_tag="VFQ35_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9931999.1"
                     /translation="SGNASDESEGAAGHAGGAANAQDDACTGEAGAGGERELSAYARL
                     RTACGAGVDVNRRGSRTTIGFNIK"
     gene            314..1621
                     /locus_tag="VFQ35_14960"
     CDS             314..1621
                     /locus_tag="VFQ35_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932000.1"
                     /translation="MLLVDPLELDGVLARYARLSARFRQALRTSDAPEHAFELPPREL
                     RDEQLAELTHALARDPLAKPLTQWTHELLVRHACLDAERRVAHARFRDERPFDGPERG
                     RFTPQALLERSLSDAGRRRAWLEALARHTSSLASARFALWETRAQALESFPEAAPSGE
                     RAELVSSAAARLLDSSRDAATSLGLDSFESWVSLGVGVDAIGDYPTRLSARSLVELLG
                     ERAWLHGLEPELRGVPALLGSSSVLRGMAQLGEALHNAAARERRPFVANHDPFEQRAL
                     LFGALFGMLPSLRPFAERRLAIARARLGDHERSLMRVVLLGARALALRALLAGYEARG
                     ERSYREAFAEQTARCFGFELEPVFAGALFVRRRAREELAAFLLALVESSELAERHDED
                     WFRNPRAIAELRARLESPPELSFDEARLTRGTELLLERLRAAL"
     gene            complement(1867..2874)
                     /locus_tag="VFQ35_14965"
     CDS             complement(1867..2874)
                     /locus_tag="VFQ35_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932001.1"
                     /translation="MSSRVRTLKRQRFASTGAAAIGFASLALFATRPAFADSSDLDPA
                     IGYNYGEIETARTAATGGAQRALSTSIGALFINPANIAQERVYHIGAFAQLWPEAHRQ
                     SYGAAATDSVSSGSHLAAAVGATYNFQDPDGIDRRWTDLRVALAYPFSDQFSFGLGGR
                     YLWLSQNGLGPLGKSPVSNGLSGEQIVRNFSFDAGATFRPVSELSLSVVGVNLSNPGH
                     GFFPTSLGGGIGYGGKQVALEGDLVADFTTWESTKLRAMTGLEVLFADHYSLRGGYRY
                     DQGPGSHGLSLGVGYIDRMFILDLAVRRTVTGGDATAVVLGFTYHLESAGMTPTMGET
                     F"
BASE COUNT          511 a         1100 c         1049 g          517 t
ORIGIN      
        1 gcagcggaaa tgcgagcgac gagtccgaag gcgctgccgg acacgccggc ggcgccgcga
       61 acgcccaaga cgatgcgtgt acgggtgagg ccggcgctgg aggcgagaga gagctctctg
      121 cctatgctcg ccttcgcacc gcgtgtggcg ccggggtcga cgtaaaccgg cggggttccc
      181 gcactaccat cggcttcaac atcaagtgat gccatccccg ccgccgcgac aagcgtcggt
      241 tctatccccc aagctcggtt ccgctgcgcg agtcgagcgc tcgcccgaga tggtgtaaga
      301 gcaatgtacc gccatgctct tggtagaccc gctcgaactc gacggcgtcc ttgcccgtta
      361 cgcgaggctc tccgcgcgtt tccgccaagc cttgcggacg agcgacgccc ccgaacacgc
      421 gttcgagctg cccccccgcg agctccgcga cgaacagctc gccgagctca cccacgctct
      481 cgcgcgcgat ccgctcgcga agccgctcac gcagtggacg cacgagctct tggtgcggca
      541 cgcctgcctc gacgcggagc gccgagtcgc gcacgcacgc tttcgcgacg aacgtccgtt
      601 cgacggcccc gagcgcggtc gcttcacccc gcaagcgctg ctcgagcgca gcctctctga
      661 cgctgggcgc cgtcgcgcct ggctcgaggc gctcgcccgt cacacgtcga gcttggcgtc
      721 cgcgcgcttc gcgctgtggg agacacgcgc ccaggcgctc gagagctttc cagaggcggc
      781 cccgtcgggc gagcgcgcgg agctggtctc gagcgcagcc gcgcgtttgc tcgattcttc
      841 gcgagacgcg gcgacgagcc tcggcctcga ctctttcgag agctgggttt cactcggcgt
      901 cggtgtcgac gcgatcggcg attacccgac gcggctcagc gcgcgcagct tggtcgagct
      961 tttgggagag cgagcttggc tgcacgggct cgaaccggag ctccgcggcg tgcccgcgtt
     1021 gctcggcagc tcgagcgtgc tgcgcggcat ggcccagctc ggggaggcgc ttcacaacgc
     1081 ggccgcgcgc gagcggaggc cgttcgtcgc gaaccacgat ccgttcgagc agcgcgcgct
     1141 gctcttcggc gcgctcttcg gcatgctgcc gtccttgcga ccgttcgccg agcgtcgcct
     1201 cgccatcgcc cgagcgcgcc tcggggatca cgagcgctcc ctcatgcgag tcgtgttgct
     1261 cggcgcgcgg gccttggcgc tccgagcgct gctcgcgggc tacgaagcgc gcggtgagcg
     1321 cagctaccgt gaagcgttcg cggagcaaac agcgcgctgc tttggcttcg agctcgagcc
     1381 cgtgttcgcc ggcgcgcttt tcgttcggcg ccgcgcgcgc gaagagctcg ctgcattttt
     1441 gctggcgctc gtcgagagct cggagctcgc cgagcgacac gacgaggatt ggttccgtaa
     1501 ccctcgcgcc atcgccgagc tgcgcgcccg cctcgagagc ccaccagagc tcagcttcga
     1561 cgaagcgcgc ctcacgcgcg gtacggagct actgctcgag cgcctgcgcg cagcgctgtg
     1621 agctcgcatc ggctctgcgc agcgctcgag ctcagcttcg acgaagcgcg cctcacgcgc
     1681 ggtacggagc tgctgcttga gcgcctgcgc gcggcgctct gagctcgcat tggctctgcg
     1741 cagcgctcgc tggggccgcg cttggatgcg cacgcgggcc gacgagggtt gaggcgatgc
     1801 ctcggagaat catcagggcg ggcgcgacag cgcgaacacc cccaattggc cgagcgctac
     1861 ctatcgctaa aacgtttcgc ccatcgtcgg agtcatgccc gccgactcga ggtgatacgt
     1921 gaagccgagc acgaccgcgg tcgcgtcgcc gccagtaacg gtgcgtcgaa ccgcgaggtc
     1981 gaggatgaac atgcggtcga tgtagccgac gccgagcgac agaccgtggc tacccggtcc
     2041 ttgatcgtag cgatagccgc cacgcagcga gtagtgatcc gcgaagagca cttcgaggcc
     2101 tgtcatcgcg cgcagcttcg tcgactccca ggtcgtgaag tccgcgacga gatcgccctc
     2161 gagcgcgact tgcttgcctc cgtagccgat gcctccgccc aagctggttg ggaagaatcc
     2221 gtggccaggg ttcgagaggt tcacgccgac gaccgaaagc gagagctccg aaacggggcg
     2281 aaatgtcgcg cccgcatcaa aggagaagtt gcgaacgatc tgctcgccgg agaggccgtt
     2341 cgaaaccggg ctctttccga gcgggccgag gccgttctgg ctcaaccaca aatatcgacc
     2401 accgagcccg aacgaaaact gatccgagaa cggatacgcg agcgcgacgc gcaagtcggt
     2461 ccatcgccga tcgatgccgt cgggatcttg gaagttgtac gtggcgccca ccgccgccgc
     2521 gagatgcgaa cccgagctca ccgaatccgt agctgccgct ccgtaacttt gacgatgcgc
     2581 ctcgggccac agctgcgcga acgcgccgat gtgatagacg cgctcctgcg cgatgttcgc
     2641 ggggttgatg aacagcgcgc cgatcgacgt cgacaatgcg cgctgcgccc caccggtggc
     2701 ggcggtgcgc gccgtctcga tctcgccgta gttgtatcca atcgcgggat cgaggtcgct
     2761 gctgtcggcg aacgccggcc tcgtcgcgaa gagcgcgagc gatgcgaacc caatcgccgc
     2821 cgcgcctgtc gaggcgaagc gctgcctctt cagggtgcga acgcgagagc tcatggtttc
     2881 ggagaaaccg cgtctagcgc gatacgatcg aaacggccaa aatttctgcc ttctctcgag
     2941 cgcaagcgct cttcgcgcat ttcgagcgga tttgctccga acgcgcgttc gctccgcccc
     3001 gcgctggtcc gccgtgccag gtcgctggca ccgcgcatgc aacttcgctc gaaacgtgaa
     3061 gcgaaaagtc tcgcgacaat ttttcgctcg cgaagcgccg cgacgatcct gcaaccggtg
     3121 cgccctcgag cgcactcgct tcacacaaat gtcgacaatc ggaatcgcgt cacgcga
//
LOCUS       DASTKS010000723         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1902261, whole genome shotgun sequence.
ACCESSION   DASTKS010000723 DASTKS010000000
VERSION     DASTKS010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902261"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..916)
                     /locus_tag="VFQ35_14970"
     CDS             complement(17..916)
                     /locus_tag="VFQ35_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET9932002.1"
                     /translation="MKNQLKTILLLGSLSAIVIGVGALVAPGYLYVSAALALAMNLGA
                     YFFSDRIVLKMHRAAEVTPAQAPDLHEMVEELAEKAGIPKPRVYVIPEEQPNAFATGR
                     NPERGVVAVTEGIVRILDRRELRGVIAHELAHIKNRDILVSSIAAAVASLVTYAAQAL
                     SLSSLFGGNNDDEESQGSAAGGILVALVAPLAATLVQLGISRAREYMADETGARISGD
                     PEALASALLKLEQAASVVPVNAAPATASLFIVNPLNAAQTVFKLFSTHPATEERVQRL
                     LAMRAPARRAAARPYTSRLVRGF"
     gene            complement(913..1248)
                     /locus_tag="VFQ35_14975"
     CDS             complement(913..1248)
                     /locus_tag="VFQ35_14975"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9932003.1"
                     /translation="MKIVVVFRGIEASDALREHVVLRAQRELQRFSDEVSSVVVRVQD
                     INGPKGGIDKRCHVTVRGRAMAPVVIEAMSADPYSAAESGLERAARAVRREIARARGR
                     RDDGFGRAS"
     gene            1347..2285
                     /gene="nhaR"
                     /locus_tag="VFQ35_14980"
     CDS             1347..2285
                     /gene="nhaR"
                     /locus_tag="VFQ35_14980"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HET9932004.1"
                     /translation="MNDINSRRLEWVNYHHLLYFWVVAREGGLVPAGRVLRLSHPTLS
                     AQVHALEDHLGEKLFTKVGRKLALTEVGRVVFRYADEIFTLGREMVDTVKGRATGQPL
                     RLNVGVVDVVPKLVVRRLLQPALSLPEPVKLVCYESSYERLLGDLAMHTLDIVIADSP
                     VPSGSSVRAFNHPLGETGVTFFGTKSLASSLKKGFPKSLEGAPLLLPLESSPLGRALN
                     QWFERNGVKPRVVGEFEDSALIKVFGADGIGLFPAPAAVEDEVCRQYGVQVVGRAPEV
                     KERFYAVSVERRLKNPAVVAITESARQELFVESTRK"
     gene            complement(2338..2727)
                     /locus_tag="VFQ35_14985"
     CDS             complement(2338..2727)
                     /locus_tag="VFQ35_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9932005.1"
                     /translation="MDDERRRAPRAPIELKIEYKRLNTFFADYTKNISRGGTCLATEK
                     PLSVGTEFVFALGVPGMPEPVRLRGKVIWVTPTHEATKANPAGMGIEFQYDTAERLEK
                     EAAVERLMLAELGEHLTTRLLGRKPQV"
     gene            2914..3996
                     /gene="dnaJ"
                     /locus_tag="VFQ35_14990"
     CDS             2914..3996
                     /gene="dnaJ"
                     /locus_tag="VFQ35_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9932006.1"
                     /translation="MRDPYEVLGVDRSATEAELKAAFRKQAAQHHPDRNPDDPDAAER
                     FKELNQAYQILSDPQKRAAYDRFGESAFRPGGAPMPDFGGFEDLFGDLLGAFGFKNGA
                     DKGVIRKTVQLTFEEAAHGCEKEISYERIDLCARCNGNGGAPGSRSETCGACGGRGKV
                     RFQQALFPLAMERPCSRCRGTGKIPLSPCLGCQGSGLAKATRRLEVQIPAGIEAGAVQ
                     TVEGAGNRVRPDRPASALELVVEVEAHPFFRRVGDDVICKIPISYSQAALGGEVEVPT
                     LEGNVRLRVPPATQVGSVLRLRGKGIAHRLRGGHGDQLVEVTIEVPSNLSERARQLLV
                     ELGQELGDDVQPQTASFMEKLKGLFG"
     gene            4075..>4428
                     /locus_tag="VFQ35_14995"
     CDS             4075..>4428
                     /locus_tag="VFQ35_14995"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9932007.1"
                     /translation="MDTTKIGMLGAGNMAGALIRGMLASQRVAPDQIRASDVREERLS
                     ELEAEYGIVTHQDNRELIDWANLVVIAVKPQVIDRVLDSLGPSFRPGTLVVSIAAGVP
                     IRALESRLPESVRVVR"
BASE COUNT          762 a         1456 c         1449 g          761 t
ORIGIN      
        1 ctcgtggtcg tgaaggtcag aagccgcgca cgaggcgcga agtgtaaggg cgcgcggctg
       61 ctcggcgcgc gggcgctcgc atcgcgagca agcgctgaac gcgctcttcg gtcgcggggt
      121 gagtcgagaa tagcttgaac accgtttggg cggcgttcag cgggttcacg atgaagaggc
      181 tcgcggtagc aggagccgcg ttgacgggca cgacgctcgc ggcctgctcg agcttcagga
      241 gcgcactcgc caaagcttcc ggatcgcccg agatgcgagc acccgtctcg tcggccatgt
      301 actcacgcgc ccgcgagatg ccgagctgga ccagagtggc ggcgagcggc gcgaccagcg
      361 cgaccaaaat tccgcccgcg gcgcttccct gcgactcctc gtcgtcgttg ttgccgccga
      421 agaggctgct caagctcagc gcctgagccg cgtacgtgac gagcgacgcc accgccgcgg
      481 cgatgctcga cacgaggatg tcgcgattct tgatgtgcgc gagctcgtgc gcgatcacgc
      541 cgcgcagctc acgccgatcc aatatacgga cgatgccctc ggtcacggcg accacgccgc
      601 gctcggggtt tcggcccgtg gcaaaggcgt tcggctgctc ttcggggatc acgtacacgc
      661 gcggcttcgg gatgccggcc ttctcggcca gctcctccac catctcgtgc agatcgggag
      721 cctgcgccgg agtcacctcc gcggcgcggt gcatcttgag cacgatgcga tcggagaaga
      781 agtacgcgcc gaggttcatg gcgagcgcca gcgccgcgga cacgtacaag taaccggggg
      841 cgacgagcgc gccgaccccg atgacgatcg ccgaaaggga gccgaggaga aggatcgtct
      901 tcaactgatt cttcatgacg ccctcccgaa accatcgtcg cggcgaccgc gtgccctcgc
      961 tatctcccga cgcaccgcgc gcgctgcgcg ttcgagcccc gattccgccg ccgagtacgg
     1021 gtctgcgctc atcgcttcga tcactaccgg cgccatggct cgtccgcgaa ccgtgacgtg
     1081 gcagcgcttg tcgatgccgc ccttcggacc gttgatgtct tgaactctga cgacgacgga
     1141 gctcacctcg tcgctgaagc gttggagctc ccgctgagcg cgcagcacga cgtgctcacg
     1201 gagtgcgtca gaagcttcga tacctcgaaa aacaactact attttcatcg ttcttgctcc
     1261 ttcttgcgcc acctcggcgc gacacctcct ttctgaatct ttcccatcat gtcgaccata
     1321 acgaaagttc gacgtaaact gtcgtaatga acgacattaa ctccaggcgc ttggagtggg
     1381 tcaactacca ccacctgctc tacttctggg tcgtcgcgcg cgaaggcggg ctcgtaccag
     1441 caggcagagt gttgcggctc tcccatccga cgctcagcgc gcaagtccac gctctcgagg
     1501 atcacctggg cgagaagctg ttcacgaaag tcgggcgcaa gctcgcgctc accgaggtcg
     1561 gccgggtcgt gtttcgctac gcggacgaga tcttcacgct cgggcgcgag atggtcgaca
     1621 ccgtgaaggg ccgcgcaacg ggacagccgc tgcggctgaa cgtgggcgtg gtcgacgtcg
     1681 tccccaagct cgtcgtgcga cggctcctcc aacctgcgct gtcgctgccg gagccggtga
     1741 agctcgtctg ctacgagagc tcgtacgaga ggctgctcgg cgatctcgcg atgcacaccc
     1801 tcgacatcgt catcgccgac tcgcccgttc cgtcgggcag cagcgtccgc gccttcaacc
     1861 acccgctcgg cgagacgggt gtcaccttct tcggtacgaa gtcgctcgcg agctcgttga
     1921 agaaaggttt tccgaagtca ctcgaggggg ctccgctgct gcttccgctc gagagctccc
     1981 cgctcgggcg cgcgctgaac caatggttcg aacgaaacgg cgtgaaaccc agggtcgtcg
     2041 gcgagttcga ggacagcgca ctcatcaagg tgttcggcgc ggacgggatc ggtttgttcc
     2101 cggctccggc tgcggtcgaa gacgaggtct gccgccaata tggcgtgcaa gtagtcgggc
     2161 gcgcccccga agtgaaagag cgcttttacg ccgtttcggt cgagcgccgg ttgaagaacc
     2221 ctgccgtcgt cgcgatcacc gagtcggccc ggcaagagct gttcgtcgaa tcgacccgca
     2281 aatgacgagc gtctcgcggc cttcgccgcg ctcactcgcc gctccgacca gagcgcctca
     2341 gacttgcggc ttgcggccga gcaggcgcgt cgtcaggtgc tctccgagct cggcgagcat
     2401 caatcgctcc accgccgcct ctttctccag gcgctccgcg gtgtcgtact ggaactcgat
     2461 gcccatccca gccgggttcg ctttggtcgc ctcgtgggtc ggcgtgaccc aaatgacttt
     2521 ccctcgcaaa cgcacgggct cgggcatccc cggcacgccg agcgcgaaga cgaattcggt
     2581 cccgaccgat agcggcttct ccgtcgcgag gcaggtgcca ccccgcgaga tgttcttcgt
     2641 gtagtcggcg aagaacgtgt tgaggcgctt gtattcaatc ttgagctcga tgggagccct
     2701 gggtgcgcgg cgtcgttcgt cgtccatgcc cggcttggtg gcgattgtag cggcgccttc
     2761 cgaagcaacg gaaggtgtgt tcgtgatgac ggcgcgaaag ggctgaaaaa tgccgcgtgg
     2821 gtgggtcggg gtgagcccgc tgcagccggc ccgaggtccc tcgccgaaat ctggaggccg
     2881 cggccgccgt tccagtgcta tgcaccccga aacgtgcgcg atccctacga agtgctcggt
     2941 gtggaccgct ccgcgaccga ggcggagctg aaggcagcct ttcgcaagca agccgcgcag
     3001 caccaccccg acagaaaccc cgacgacccc gacgccgccg agcgcttcaa agagctcaat
     3061 caggcgtatc aaatcctgag cgatccccag aagcgagccg cgtacgatcg gttcggagag
     3121 agcgcatttc gtcctggtgg cgctccgatg ccggatttcg gaggcttcga agacctgttc
     3181 ggtgacctgc tcggcgcctt cggcttcaag aacggcgccg acaagggcgt catccggaag
     3241 acggtgcagc tgaccttcga agaggcggcg cacggctgcg agaaggaaat ctcctacgag
     3301 cgcatcgacc tttgcgcccg ctgcaacggc aacggcggcg cgcccggtag ccggagcgaa
     3361 acctgcggag cctgcggtgg gcgcgggaaa gtgcgctttc agcaggccct gttccctctc
     3421 gccatggagc ggccgtgttc gcgctgccgc ggcaccggca agatcccgct ctcgccttgt
     3481 cttggttgtc agggcagcgg gcttgcgaaa gcgacgcggc gcctcgaggt gcagatcccg
     3541 gcgggcatcg aagcgggagc cgttcaaacg gtcgaaggcg ccggcaatcg cgtgcgtccg
     3601 gaccgccccg cgagcgcgct cgagctcgtg gtggaggtcg aagcgcaccc gttcttccgc
     3661 cgcgtcggcg acgacgtcat ttgcaagatt ccgatctcgt atagccaggc agcgctgggc
     3721 ggcgaggtcg aagtgcccac cctcgagggc aacgtgcggc tgcgcgtccc ccccgcgact
     3781 caggttggca gcgtgcttcg gctgcgagga aaaggcatcg cgcaccgcct gcgcggtgga
     3841 cacggcgatc agctggtcga ggtgaccatc gaggtgcctt cgaacctcag cgaacgcgcg
     3901 cgccagttgt tagtcgagct cgggcaagag ctcggtgacg acgtgcagcc ccaaaccgcg
     3961 agcttcatgg aaaagctcaa gggactgttc ggctgaggcc gaccgcgccc actagagaac
     4021 ctcgagaccg ctcgcccgag ggcgacacga ctccgctacg aaagaacgac gacgatggac
     4081 acgaccaaga ttgggatgct cggcgccggc aacatggctg gcgctctgat tcgagggatg
     4141 ctcgccagcc agcgcgtggc tccggaccaa atccgcgcga gcgacgtgcg tgaagagcgc
     4201 ctttcggagc tcgaggccga gtacggcatc gtcactcatc aggacaatcg cgagctcatc
     4261 gactgggcga acttggtcgt cattgcggta aagccgcagg tcatcgatcg cgtgctcgac
     4321 agcctgggac catcgttccg ccccggcacg ctcgtcgttt cgatcgcggc gggcgtaccg
     4381 attcgcgcgc tcgagtcgcg cttgccggag agcgttcgcg tcgtgcgt
//
LOCUS       DASTKS010000724         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_116101, whole genome shotgun sequence.
ACCESSION   DASTKS010000724 DASTKS010000000
VERSION     DASTKS010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116101"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFQ35_15000"
     CDS             complement(<1..237)
                     /locus_tag="VFQ35_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9932008.1"
                     /translation="MGVKTNAARILDSLGISYELRNYEVDESDLSATSVARKVGLPPE
                     QVWKTLVARGDRHGVCFAVVAADAELDLKALARAT"
     gene            306..1163
                     /locus_tag="VFQ35_15005"
     CDS             306..1163
                     /locus_tag="VFQ35_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9932009.1"
                     /translation="MTSPLLTNGPLELLYEERGLPSFDLGARLREAYGGGSLGFERPA
                     LIANFVSSVDGVVALPSGGESGMVISQSSAADHFVMGLLRMVAGAVVVGASTLRRSPG
                     HRWTAEAIYGPAAADYASTRRALGLSPQPTFVVVSASGNIDTSQPALENAIVVTTGAG
                     AALLESRARSHCRVIAFEGARVDMKAVLRTLRSEGHELVLTEGGPSLFAELLHEGLVD
                     ELFLTTSPVLFGRYASDQRKSLAEGLDLAGTALELRSLRRHGSHLFHRYRLKRDASDK
                     ESASRSAAS"
     gene            1169..2332
                     /gene="tgt"
                     /locus_tag="VFQ35_15010"
     CDS             1169..2332
                     /gene="tgt"
                     /locus_tag="VFQ35_15010"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9932010.1"
                     /translation="MLLPFTVEAVDVHSRARRGRLLTAHGPVETPAFMSVGTRATVTG
                     LDPDDLKAVGANVLLANTYHLLLRPGPALFRRVGGVHAFMRWPGPILTDSGGYQIFSL
                     PGDRTITEKGARFKSYVDQRTYLLSPESSIDMQTALGSDIMMVLDECVASTSDEATTR
                     QAMERTHRWALRSLAARTNPAQALFAIVQGGVVPALRRESAAFLSAHPFDGFALGGLA
                     VGDTRTQREDITAFAAELLPGERPRYLMGVGTPPDLLYAMLAGIDMFDCVLPTHLGWQ
                     GTAFTSTGRVRVTRSACASSDVPLDATCGCSTCARFTRAYIHHLFKCSEPLGPRLLSI
                     HNLHHYLALMSEAREAISQGRYAQYARAKLDQIDRHEHNGKRLGRTDALEAFG"
     gene            2329..3153
                     /locus_tag="VFQ35_15015"
     CDS             2329..3153
                     /locus_tag="VFQ35_15015"
                     /inference="COORDINATES: protein motif:HMM:NF017266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HET9932011.1"
                     /translation="MTLDCEVVLTRSGEPAMRDRISGEVMHPVVGPRVESRLLYAEGS
                     KLRERLLGSGPGALLLLDVGLGAGSNAVAALEVALALQGKGRPLRVVSFDQSLSALEQ
                     ALRPEHREAFGYSGALLEAGLAVLRDRKYCSESVTWELELGTLPDTLARIDAGSADVV
                     YWDPFSPAKNPTLWTVATFSELWTRCRDGATFFTYSAATATRSALLLAGFAVGEGEST
                     GVGKRTTQAAANVRDLARPLQRRWLETLSRSSAPLPSDAPIDALDRIRAAAQFCIG"
     gene            complement(3199..3870)
                     /locus_tag="VFQ35_15020"
     CDS             complement(3199..3870)
                     /locus_tag="VFQ35_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9932012.1"
                     /translation="MSSQAFTRNYSDHSNDTGFQFEFFCDKCGNGHRSSFKHNALGVA
                     ASMIKAAGALFGGAAYRAGWGADHVKDALRGPAWDSAFKEAVEECRPKFRQCTRCGKW
                     VCPEVCWNEPRGLCEECAPDLSEHAPMIQAQVAVEQAWEQARKGDQLRGADLNAAPMR
                     VGAEVECVKCRTTLGPNARFCAQCGTPVAAAPRAATGPKFCAGCGTKLDSGARFCPGC
                     GSAVK"
     gene            4501..>5479
                     /locus_tag="VFQ35_15025"
     CDS             4501..>5479
                     /locus_tag="VFQ35_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9932013.1"
                     /translation="MTLAPPPQRTRKRPGGPAIGILGNAFGDPYSIPLTQAATQRIAQ
                     SGVHTLCFTGGFPNAPFFRSNDGRLSVPEAIDAMILLSATLRGFDEDLKQMVDESSAP
                     IISVGANLARASSITADDETGVFQAIAHLVKRHECKRIAFIAGPEGSFDGARRLTAYR
                     IALDSFKLPWDPTLAVRGDYEARSGREAVLELRRNGSRSFDAIVAANDLMAIGAIEGL
                     RAGGMRVPEDVKVVGFDDMEEASFTSPSLTTVRQPITEQGVCAADVALRELGGEALSE
                     IPTHISTPLVIRQSCGCASASNSETTRRIAKPTGAEAEDRELVGDALRGI"
BASE COUNT          919 a         1921 c         1694 g          945 t
ORIGIN      
        1 ggttgcccgc gcgagagctt tgagatcgag ctcagcatcc gccgcgacga cggcgaagca
       61 gacaccgtgg cggtcgccgc gcgcgaccaa cgtcttccag acttgttcag gcggcaagcc
      121 caccttgcgt gcgacgctcg tcgccgataa atcgctctcg tcgacctcgt aattccggag
      181 ctcgtaagag ataccgagcg aatccagaat gcgcgcggcg ttggtcttca cgcccatcgc
      241 ttaccgcaaa acctcggcgc cgcacgaacc gtgctaagag accggccgcc ttcgcgcaga
      301 tccgcatgac ttccccgctt ctaaccaacg gtcctctcga gctcttgtac gaagagagag
      361 gcctcccctc gttcgacctc ggcgcacgcc tgcgagaggc ctacggcggc ggcagcctcg
      421 gtttcgagcg ccccgcgctc atcgcgaact tcgtgagctc cgtcgacggc gtcgtcgccc
      481 tgccctcggg cggcgagtcc ggcatggtca tcagccagag cagcgcagcc gaccacttcg
      541 tcatggggct cttacgcatg gtcgcgggcg cggtggtcgt cggcgcgagc accctgcggc
      601 gctcacccgg acaccgctgg acggcggagg ccatctacgg accagccgcg gccgattacg
      661 cgagcacccg gcgtgcactc gggctctccc ctcaaccgac gttcgtcgtg gtgagcgcgt
      721 cgggtaacat cgatacgagc caaccagctc tcgagaatgc catcgtcgtc acgaccggcg
      781 ccggcgcggc gctcctcgaa agccgcgcgc gttcccactg tcgcgtcatt gcgttcgaag
      841 gagcgcgcgt cgacatgaag gcggtgcttc gtacgttgcg aagcgagggc cacgagctcg
      901 tcctcaccga aggagggccc tctctcttcg ccgagctact tcacgaaggg ctcgtcgacg
      961 agctcttcct cacgacgtca ccggtcttgt tcggccgcta cgcgagcgac cagcgcaaat
     1021 ccctcgccga agggctcgat ctcgcgggca ccgcgctcga gcttcggagc ttgcgcaggc
     1081 acggctccca cctgttccat cgctaccgat tgaagcgcga cgcgagcgac aaagaaagcg
     1141 cgtctcgctc cgcagccagc tgagcaccat gctactgccc ttcaccgtcg aggccgtcga
     1201 cgtccactcc cgcgcacgcc gcggtcggct gctcaccgcg cacggaccgg tcgagacccc
     1261 tgcgttcatg agcgtaggga cgcgtgcgac cgtaaccggg ctcgaccccg acgacctcaa
     1321 ggcagtcggc gcaaacgtgt tgctcgccaa tacgtatcac ctgctgctgc gccccgggcc
     1381 cgcgctcttc cgacgcgtgg gcggggtgca cgctttcatg cgctggccag gccccatcct
     1441 caccgattca ggcggctacc agatcttttc gctgcccggc gaccgcacga tcacggagaa
     1501 gggcgcgcgc ttcaagagct acgtcgacca gcgcacgtac ctgctctcac ccgagagctc
     1561 gatcgatatg cagacggcgc tcggctcgga catcatgatg gtgctcgacg agtgcgtcgc
     1621 ttcgacctcg gacgaggcca ccacacgcca agcgatggag cgcactcacc gctgggccct
     1681 ccgcagcttg gccgcccgca cgaaccctgc gcaagcactg ttcgcgatcg tccaaggcgg
     1741 cgtcgtgccc gcgctgcgcc gagaatctgc cgcgttcttg agcgcgcatc cgttcgacgg
     1801 cttcgcgctc ggcggcctcg cggtgggcga cacgcggact cagcgtgaag acatcacggc
     1861 cttcgcggcc gagctcctgc cgggcgaacg ccctcgctac ttgatgggag tcggcacgcc
     1921 ccccgatttg ctatacgcga tgctcgccgg catcgacatg ttcgactgcg tgctgccgac
     1981 tcatcttggc tggcaaggca ccgcgttcac gtcgacgggc cgggttcgcg tgacgcgaag
     2041 cgcctgcgcg tcgagcgatg tccccctcga cgcgacgtgc ggttgctcca cctgcgcgcg
     2101 cttcactcgc gcctatatcc accacctctt caagtgcagc gagccgctcg ggccacggct
     2161 cctctcgatc cataacttgc accactacct tgcgttgatg agcgaagcgc gtgaggcgat
     2221 ttcgcaaggg cgctacgccc aatacgcgcg ggccaagctc gatcaaatcg accgacacga
     2281 gcacaatggc aagcgcctcg gtcgaaccga cgcgctcgag gctttcgggt gacgctcgac
     2341 tgtgaagtgg tgctcacgcg cagcggcgaa ccggcgatgc gcgaccggat ctcgggcgaa
     2401 gtgatgcacc ccgtcgtcgg tccgcgcgtc gagtcgcgtc tcctttatgc ggaagggtcg
     2461 aagttgcgcg aacggctgct tggctcgggg cccggcgctt tactccttct ggacgtcggg
     2521 ctcggcgccg gatcgaatgc ggtggccgca ctcgaggtgg ctttggcgct ccaaggcaag
     2581 ggtcggccgc tccgcgtcgt gagcttcgat cagagccttt ccgcgctcga gcaagcgctt
     2641 cgccccgagc accgcgaggc cttcggctat tcgggagcgc tgctcgaggc tggcctcgcc
     2701 gtgttgcgcg accgcaaata ttgctctgaa tccgtgacat gggagctcga actcgggact
     2761 ttgcccgaca cgctcgcgcg catcgatgcc ggttccgccg acgtcgtgta ttgggacccg
     2821 ttctctccgg cgaaaaatcc gactctctgg accgtggcga ccttctccga gctctggacg
     2881 cgttgccgcg atggcgcgac gtttttcact tacagcgcgg cgacggcgac gcgctccgcg
     2941 ttgctgctcg ccggcttcgc ggtgggtgaa ggcgagtcga cgggggtcgg caagcggacg
     3001 acgcaagccg ccgcgaacgt ccgcgatctc gcgcggccgc ttcagcgccg ctggctcgaa
     3061 acgctgtctc gctcgagcgc accgctgcca agcgacgctc caatcgatgc gctcgaccgc
     3121 atccgtgcgg cggcccagtt ctgcatcggc tgaaggccgc gcacgacgag cgccaaagcg
     3181 cgctctacga gactgtgctc acttcacggc ggagccgcag ccagggcaaa agcgcgcccc
     3241 gctgtcgagc ttggttccgc agcccgcgca aaacttcggc cccgtcgccg ctcgaggcgc
     3301 agccgcgacc ggcgtcccgc attgcgcgca gaaccgcgcg ttgggaccga gagtcgtgcg
     3361 gcacttcacg cactcgacct cggccccgac gcgcatgggc gcagcgttca agtcggcgcc
     3421 gcgcagctgg tcgcccttcc ttgcttgttc ccaggcttgc tcgaccgcga cctgcgcttg
     3481 gatcatggga gcgtgctcgc tcaaatccgg agcgcactct tcgcaaagcc cgcgcggttc
     3541 gttccagcag acctcggggc acacccattt gccgcaccgg gtgcattgac gaaacttcgg
     3601 ccggcactcc tcgaccgcct ctttgaaggc gctgtcccat gccgggcccc tcagggcatc
     3661 cttcacgtga tcggcgcccc accccgcgcg ataggcagcg ccgccgaaca gcgctcccgc
     3721 cgctttgatc atcgacgcgg cgacgccgag cgcgttgtgc ttgaagctcg agcggtgacc
     3781 atttccgcac ttgtcgcaga agaattcgaa ctgaaaaccg gtatcgttcg aatggtcaga
     3841 atagttgcgc gtgaacgcct gcgagctcat cggcgcagga tcatagtcaa aacccgcgtc
     3901 actcgcgcgg caaagtggcg aaggccgcgc ctccgcccgt tacgtgctgc aatcatccgg
     3961 acgcttgaga agcggcgacc tccctcgcga aaggtcgccg cggcggaacg aaagcagccg
     4021 gcgcttcact tgcgcgcggc tttctccttc ttcttttcct tcttctcttt cggggtgagt
     4081 ttcggcttgt tgttcgtttt gggccgctgc tgacctttgg ccatggtgct aactccttcg
     4141 cggaacgccc ggatagtagc gcactttctc cgatttcgat ccggtgcgtc attcgcgcca
     4201 gtcgccggtc gatcgcggac cgccctacag cccgacgctc acccggcgag cgacgcggat
     4261 tccgctcgac gacttcgccc agcgtgaaga ggcggccgaa ccgcagcaga gccctcgaac
     4321 tgccgagcag cgtggaccga tgtaggcacc atggtgggcg gcatgtaggg ccgcgggggc
     4381 caagcaggag tccttacgaa cccttaacac ccgacaagaa caaggctatt tcaaatggtg
     4441 cgcttcgccg aacatttttt cggaagagca ccaccccatt ttcgaagcgg gcaactacgc
     4501 atgactctcg ctccccctcc gcaacgaacg cgaaaacgcc caggtggccc tgcgatcggc
     4561 atcctcggca acgccttcgg agacccttac tcgatcccgc tgacgcaagc cgcgacccaa
     4621 cgcatcgcgc agagtggcgt tcacaccctc tgcttcaccg gaggctttcc gaacgcccca
     4681 tttttccgaa gcaacgacgg gcggttgagc gtccccgaag ccatcgacgc gatgatcctg
     4741 ctctccgcaa cgttgcgggg tttcgacgaa gacctaaagc aaatggtcga cgagagctcc
     4801 gccccgatta tcagcgtggg cgccaatttg gcgcgtgcct cgagcatcac cgccgacgat
     4861 gaaaccggcg tgtttcaagc catagcgcac ctcgtgaagc gacacgaatg caagcgcatc
     4921 gccttcatcg cggggccaga gggcagcttc gacggagcgc ggcggctcac cgcctatcgc
     4981 atcgcgctcg acagcttcaa gttgccctgg gacccgaccc tcgccgtccg cggcgactac
     5041 gaggcgcgtt cgggacgcga agcggtgctc gagctgcgcc gaaacggtag tcgcagcttc
     5101 gatgcgatcg tcgccgcgaa cgatctcatg gcgattggcg ccatcgaagg cctgagagca
     5161 ggcggcatga gggttccgga agatgtcaaa gtcgtcgggt tcgacgacat ggaggaggct
     5221 tcgttcactt caccttccct caccaccgtc cgacagccca tcacggagca aggcgtctgc
     5281 gccgccgacg tcgcgctgcg cgagctcggc ggcgaggccc tgagcgagat cccgacgcac
     5341 atctcgactc cgctcgtcat tcggcaatcg tgcgggtgcg ccagcgccag caactcggag
     5401 acgacgcggc gcatcgcgaa gcctaccgga gccgaggcgg aggaccgcga gctcgtcgga
     5461 gacgctttgc gcggtatcg
//
LOCUS       DASTKS010000725         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1456427, whole genome shotgun sequence.
ACCESSION   DASTKS010000725 DASTKS010000000
VERSION     DASTKS010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456427"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2086
                     /locus_tag="VFQ35_15030"
     CDS             <1..>2086
                     /locus_tag="VFQ35_15030"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9932014.1"
                     /translation="DGQADALRFLDALGARIANYPKNLVAKVRVDSRAEREFAETYAL
                     SLMDPEDVRTLREAVERRRDWDVSRQLDLDLDGDSDEPRPEIPLDELMKKYEARFGKP
                     RAGSGDHFVSADGKSAVLLVQVASHDTGQKSDEELIRRVQADVAELKFPSAFGGGLRV
                     GYAGDVAARVEEARGLESDLTLSSLVVLLLVGGSLVFFYRSAWSIPILCLPLALGTFT
                     AFALVALPPLGIVSLNTNTAFLGSIVVGNGINSGIILLARFLEERRGGFDVDASVERA
                     VSGTWKATLAASLAAAVAYGSLVFTAFRGFNQFGWIGAFGLLSCWVANYTLLPVLAKL
                     WGGRLGTLHLAKESKERSGLTRWALDHARGVLILTLAASVSAGVGLVQRSGDWIEYDL
                     SKLRRRDSWESGERYWGKRMDAALGTYLTPTVIMAGSDDEARLIEARLVDLKANSGAG
                     GLIGSVRSAELFRSPARDVSRAEARKLKAVLTPRLLAELSPKDRALVERALSPRSLEP
                     FSAKDIPETLLTGLRQRDGSFAPNVLVFPKLGVGTWNATLLDAYSNDLREAATINGTA
                     RPVAGSLLLSSDIARAMNADGPKATRISLLAVLAICLLAFRATGRGHATGFARESFGY
                     SLSAMASLALGILLMMGLLAWSSQRINFSNFVALPITFGVGADYAINVLRRYQAESKN
                     SLSHALDSTGGAV"
BASE COUNT          313 a          705 c          686 g          382 t
ORIGIN      
        1 ccgatgggca ggcggacgcg ctccgcttcc tcgatgcgct cggtgcgcgc atcgccaatt
       61 accccaagaa cctggtggcc aaggtgcggg tggactctcg cgccgaacgc gagttcgccg
      121 agacctacgc gctcagcttg atggatccag aagacgtccg cacgctgcgc gaagcggtgg
      181 agcgacgacg cgactgggac gtctcgcgtc agctcgactt ggatctcgac ggggacagcg
      241 acgaaccgcg cccggagatc ccgctcgacg agctgatgaa gaagtacgag gcgcgcttcg
      301 gaaagccgcg cgccgggtcg ggcgaccact tcgtctccgc cgatggcaag agcgcggtgc
      361 tgctcgtgca ggtcgcgagc catgacacgg gtcagaagag cgacgaagag ctgattcggc
      421 gcgtgcaggc cgacgtcgcc gaactgaagt ttccgtctgc gttcgggggt ggcttgcgcg
      481 tcggctacgc tggcgacgtc gcggcacgcg tcgaagaagc gcgcgggctc gagtcggatc
      541 tcactctgtc aagcctggtc gtgctgctgc tggttggcgg ctcactcgtg ttcttctatc
      601 gctcagcgtg gtcgattccg atcttgtgcc ttccgctcgc gctcgggacc ttcaccgcgt
      661 tcgcgctcgt cgcgctgccg ccgctcggga tcgtctcgct caacacgaac acggcgttcc
      721 tcggctcgat cgtggtcggc aacggcatca atagcggcat catcctcctc gcgcgctttc
      781 tcgaagagcg gcgcggcggg ttcgacgtcg acgcgagcgt cgagcgcgcc gtttccggga
      841 catggaaagc gaccctcgcc gcatcgctcg ccgccgcggt cgcttacggc tcgctcgtct
      901 tcaccgcttt ccgcggcttc aaccagttcg gctggatcgg cgccttcggg ctcttgagct
      961 gctgggttgc caactacacg ctcttgccgg tgctcgcgaa gctctgggga gggcgcctcg
     1021 gcaccctcca cctcgcgaag gaatcgaaag aacgttcggg gctcacgcgt tgggcactcg
     1081 accatgcgcg cggcgtgctc atcctcacac tggcggcgag cgtctcggcc ggcgtcggcc
     1141 tggtgcagcg ttctggcgac tggatcgagt acgatctttc gaagctgcgg cggcgcgact
     1201 cctgggagag cggcgaacgc tactggggca agcgcatgga cgccgcgctc ggcacctatc
     1261 tcacgccgac cgtgatcatg gcgggctcgg acgacgaggc acgcctcatc gaagcgcgcc
     1321 tcgtcgatct gaaggcaaac tccggagccg gcgggctcat cggctcggtt cgaagcgccg
     1381 agctctttcg ctcgcctgcg cgagacgtct cgcgcgcgga agcacgcaag ctgaaagcgg
     1441 tgcttacccc gcgtctgctc gccgagctct ctccaaaaga tcgggcgctg gtggagcgag
     1501 cgctctcgcc gcggtcactc gaaccattca gcgcaaagga cattcccgaa acgctcttga
     1561 cggggctgcg gcagcgcgac ggcagcttcg ctcccaacgt gctcgtcttc cccaagctcg
     1621 gtgtcggtac gtggaacgcg accttgctgg acgcctactc gaacgacctg cgcgaggccg
     1681 cgaccatcaa cggcacggcg cgtccggtcg ctggctcgct gctgctctcg agcgacatcg
     1741 cgcgtgccat gaacgcggat gggcccaagg cgacgcgcat ctcgctgctc gcggtactcg
     1801 cgatttgctt gttggcgttt cgcgcgacgg gtcgcgggca cgccaccggt ttcgcgcgcg
     1861 agtcgttcgg ctattcgctc tcggccatgg cgtccctcgc cctcggcatc ttgttgatga
     1921 tggggcttct cgcctggtcg tcccagcgca tcaacttctc gaacttcgtg gcattgccga
     1981 tcaccttcgg cgtcggtgcc gactacgcca tcaacgtgct ccggcgttac caggccgaat
     2041 ccaagaactc gctctcgcac gcgctcgaca gcacgggcgg agcggt
//
LOCUS       DASTKS010000726         5059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1232873, whole genome shotgun sequence.
ACCESSION   DASTKS010000726 DASTKS010000000
VERSION     DASTKS010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232873"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFQ35_15035"
     CDS             <1..451
                     /locus_tag="VFQ35_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9932015.1"
                     /translation="LADVLGEKARFAGAGAAGAIPASLVAVLGARVVAGSDFVLDLIG
                     FDAALRGASLVISAEGRFDAQSAMNKGPVAVARRARARGVPTIVLAGAMETRELDETH
                     VAAALSITRTPCELDQARSSTADWLALTAEQVLRVFMLGQSAARAVP"
     gene            complement(458..592)
                     /locus_tag="VFQ35_15040"
     CDS             complement(458..592)
                     /locus_tag="VFQ35_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932016.1"
                     /translation="MYLARTKSPRKTNRKRSARLRAAAKAKNKKRRALSGTLSKRRGG
                     "
     gene            complement(678..1001)
                     /locus_tag="VFQ35_15045"
     CDS             complement(678..1001)
                     /locus_tag="VFQ35_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9932017.1"
                     /translation="MAHGRKAERVTINKEFESFDQFMSEYVTNLSRTGAFIRTSTPLP
                     VGTEVDLRFTVVLEGVEVIEGIGEVVRLETSPPGMGITFKELYGDSQALIERLLARNA
                     KAEPG"
     gene            complement(1023..3254)
                     /locus_tag="VFQ35_15050"
     CDS             complement(1023..3254)
                     /locus_tag="VFQ35_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9932018.1"
                     /translation="MPRNTLVINCDIRETRVALIEGGVIAELQIERAGARGTVGNIVL
                     GRVTRVLPGMQAAFIDVGMERAAFLHVEDLIRPDDFDAYLAGARRHDEGERWNKGEGG
                     KRRGTAPVKSSQAQDEDDDDDEEDDDDEISSPHSTAVEPEVAEDDDESDDGEEEEEEE
                     EEGEEQEIDPEEEEVAQIVDDEDDSSETIAEQEAEAEAVDADDEDEVPEAEVEVEAEA
                     DDAEVEAEADEADDDDSSAEAEADGDAPAASLNGEAVETSLARKRRRRRRRRGRGGDA
                     QKSSPTAAEAPVEARPAARPQRAERAERAERRPERQGRSERERSPKESGNDKDARRAK
                     GIPRVSRSVPITEVVKEGDEVIVQISKEPIGTKGARVTSHVSLPGRYVVYLPTVDHVG
                     VSKRIGSARERTRLRQVVDTLKPPSGGIIVRTVAEGLTKKDLKSDIGYLVRLWDDVLK
                     KRAGAKAPCVLYTELDSVLKTARDLFTDEIESIVIDDKAEYARLVRFVEMFMPSRVKD
                     IHYYSGDEPIFDAYGIEDEISRALSRKVPLPSGGYLIIDQAEALTAIDVNTGRFVGKG
                     SKDLEETALKTNLEAVEEIAYQLRFRNIGGLVILDLIDMDAPQSREKVWRALEQRLAK
                     DKAKTTINRISELGLIEMTRKRTRESLTRTMHETCFYCDGTGQIQSRTTIAYEILRQI
                     RRERHNLAGYSVVVNAHPAVVDLMTGDEREAVTEAERRYMRRIEFNPRREYHIEQFDL
                     QGK"
     gene            3554..4267
                     /locus_tag="VFQ35_15055"
     CDS             3554..4267
                     /locus_tag="VFQ35_15055"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET9932019.1"
                     /translation="MATSFLVLDIETVLDPELPIVATGEVERLPSPPHHKVMVIGALL
                     FDVGYEPRRIGVMSESKDEPGMLGDFAKLLEERRPCLVTFNGRGFDLPVIATRCLRYG
                     IPLRHYYRTREVRYRFSPEGHLDLMDYVADFGAAKPSRLDVIAKLCGMPGKVGVDGKD
                     VGPLVHAGRIQEVRNYCLCDVAQTAGVFLRVQLLRGELEREGYVAAMRKLIELIKGDE
                     RLAPVATALNEKRLLLEET"
     gene            4280..4612
                     /locus_tag="VFQ35_15060"
     CDS             4280..4612
                     /locus_tag="VFQ35_15060"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9932020.1"
                     /translation="MDRLVDKRCTACTPNTPRIEATELPAYLAQAPGFGANSEKTLIE
                     RRFEFKNYPETMIFVNAVAAIAHREDHHPDLEVSYGSCTVRFTTHAIHGLSENDFICA
                     AKVNALLA"
     gene            complement(4616..>5059)
                     /locus_tag="VFQ35_15065"
     CDS             complement(4616..>5059)
                     /locus_tag="VFQ35_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932021.1"
                     /translation="LGGLKASHLSLPLLESGYAYGSEAVQFEVGAHGAPVLAGRLRLN
                     DSVRRMGLGAKGGPFLNLMLLKLGVSFRASADHLIASTTSGGASDWVQGLLCLDLGWF
                     ATCADGFAARFAETSDSAKQTMGYGGLSFGFNLVKFGEQFTRGMR"
BASE COUNT          789 a         1680 c         1614 g          976 t
ORIGIN      
        1 cctcgccgac gtgctcggcg aaaaggcgcg ttttgccggc gcgggggcgg cgggagcgat
       61 cccggcgtcg ctcgtggcgg tgctgggcgc tcgcgtcgtc gccggcagcg atttcgtgct
      121 ggacctcatc ggcttcgacg cggcgctacg cggagcgtcg ctcgtcatca gcgccgaagg
      181 tcgcttcgac gcgcagagcg cgatgaacaa gggccccgtc gccgtagcgc ggcgggcacg
      241 agctcgcggc gtaccaacca tcgtcctcgc gggagcgatg gaaactcgcg aactcgacga
      301 aactcacgtc gccgcggcgc tgtcgatcac tcgcacacct tgcgagctcg accaggcacg
      361 aagctccacg gctgactggc tcgcgctcac ggcggagcaa gtgctccgcg tgttcatgct
      421 cggtcagagc gcagcgcgag ccgtgccgtg aagcgactca gccgccgcga cgcttgctga
      481 gcgtgccgga gagcgctcgg cgcttcttgt tcttcgcctt ggcggcggcg cgcaggcgag
      541 cggagcgctt gcggttggtc ttgcgcgggg acttggtgcg agcgaggtac atgaggggcc
      601 tctctctaat gcgaccgggc cgatttcgca agaagcggag tttccgccag ggactgaacg
      661 gaaacgcagg gcgaacgtca gcccggctct gccttcgcgt tccgcgccaa aaggcgttcg
      721 atcagggcct gcgagtcgcc gtagagctcc ttgaaggtga tgcccatacc cggcgggctc
      781 gtctcgaggc gaacgacttc accgattccc tcgatgacct cgaccccttc gagcacgacg
      841 gtgaaacgca ggtcgacctc ggtgccgacc ggtagcggcg tgctggtacg gatgaacgcg
      901 cccgtgcggg acaggttcgt cacgtactcg ctcatgaact ggtcgaacga ttcgaattcc
      961 ttgttgatgg tgactcgctc ggccttgcgt ccgtgtgcca tgagagcctc cctccccttc
     1021 ctctacttcc cctgcaaatc gaactgctcg atgtgatact cgcggcgggg attgaactcg
     1081 atgcggcgca tgtagcgacg ctcggcttcg gtcaccgcct cgcgttcgtc gccggtcatc
     1141 aaatcgacga ccgccgggtg cgcgttgacg accacggagt agccggcgag gttgtgacgc
     1201 tctcgccgaa tctgccgtag gatttcgtac gcgatggtcg tgcgcgactg aatctgaccg
     1261 gtgccatcgc agtagaagca cgtctcgtgc atcgtgcgcg tcaggctctc gcgcgtgcgc
     1321 ttgcgcgtca tctcgatgag cccaagctcg ctgatgcggt tgatcgtcgt cttggccttg
     1381 tccttggcca ggcgctgctc gagcgcgcgc cagaccttct cccggctctg cggcgcgtcc
     1441 atgtcgatca agtcgaggat gacgaggccg ccgatgttgc ggaagcgcag ctggtacgcg
     1501 atttcctcga ccgcctcgag gttggtcttg agcgccgtct cttcgagatc tttgctgcct
     1561 ttaccgacga accgaccggt gttcacgtcg atggcggtga gcgcctcggc ctgatcgatg
     1621 atgaggtagc cgccgctcgg cagcgggacc ttgcggctga gcgcgcgcga gatctcgtcc
     1681 tcgatgccgt aggcgtcgaa gatcggctcg tcgccggagt agtaatggat gtccttcacg
     1741 cgcgacggca tgaacatctc gacgaagcgc acgagccgcg cgtactccgc cttgtcgtcg
     1801 atgacgatgc tctcgatttc gtcggtgaag aggtcgcgcg ccgtcttcag caccgagtcg
     1861 agctcggtgt agaggacgca cggcgccttc gctccggcgc gcttcttgag gacgtcgtcc
     1921 cagaggcgaa ccaggtagcc gatgtccgac ttcaagtcct tcttcgtgag gccttcggcg
     1981 acggtgcgca cgatgatgcc gcccgaggga ggcttcagcg tgtcgacgac ctgtcgtagc
     2041 cgcgtgcgct cacgcgccga accgatgcgc ttcgagacgc caacgtggtc gaccgtcggt
     2101 aagtacacca cgtagcgccc cggcaacgag acgtggctcg tcacgcgtgc gcctttggtt
     2161 ccaatcggct ccttcgagat ttgaacgatg acctcgtcgc cctctttgac gacctcggtg
     2221 atcgggacgg agcgagagac gcgcgggatg cctttcgccc gtcgcgcgtc cttatcgttg
     2281 cccgactctt tcggactgcg ctcacgctcg gagcggcctt gtcgctcagg acgtcgttcg
     2341 gctcgttcgg ctcgttcggc tcgctgcgga cgggctgcag gacgcgcttc gacgggggcc
     2401 tcggcagcgg tggggctcga cttttgagca tcgccgcccc tacctcgtcg ccggcgccgt
     2461 cgtcggcgct tgcgggcgag cgacgtctcg acggcctcgc cgttcagcga cgccgcaggc
     2521 gcgtcgccgt cggcctcggc ttccgcggac gagtcgtcgt catcggcctc atcggcctcc
     2581 gcttcgacct ctgcgtcatc ggcctccgct tcgacctcga cctcggcttc gggaacttcg
     2641 tcttcgtcgt ccgcgtcgac ggcctcggct tcggcttcct gctcggcgat ggtttcgctc
     2701 gagtcgtctt cgtcgtcgac gatttgtgcg acttcttctt cttcggggtc gatctcttgc
     2761 tcttcccctt cttcttcctc ctcctcttct tcctcgccgt cgtcgctttc gtcgtcgtct
     2821 tcggcgacct ctggttcgac ggcggtggag tggggggagg agatctcgtc gtcgtcgtct
     2881 tcctcgtcgt cgtcgtcgtc ttcgtcttgg gcttggctgg atttcaccgg ggcggtgcca
     2941 cggcgtttgc cgccttcgcc cttgttccag cgttctcctt cgtcgtgccg acgcgctccc
     3001 gccaggtagg cgtcgaagtc gtcgggacgg atcaggtcct cgacatggag gaacgctgcg
     3061 cgctccatgc ccacgtcgat gaaggccgct tgcatgccgg gcagcacgcg tgtgacccgg
     3121 ccgagaacga tgttgcccac cgtaccgcgc gcaccggcgc gctcgatctg aagctcggca
     3181 attacgccgc cttcaatcag ggcaacccgc gtctcacgga tgtcgcaatt aataacgaga
     3241 gtatttcgag gcatggctcg ccgcttgggc gggcccccga cgctcagtgc gcccaggggc
     3301 agtcacgtcc gcttaccacg gggaagacca cctaggcgca gccgctctcc cgcggttcgg
     3361 ccacgtgacc acgtgcgcta gcctatgtca accacaccga aataattcga gatttaacca
     3421 gcggccggcc tgctgcgcga aacactttcg gaaggcttcg cccgcgaaat ctctcgaagt
     3481 tccgcgaaaa tcccgaggtt ttggggaacg cgccgattga cccgtcgcga gtccccctca
     3541 atgctccggt cgcttggcga cgtcattcct cgtcctcgac atcgagaccg tgctcgaccc
     3601 ggagctgccc atcgtggcga cgggtgaagt cgagcggctg ccctcgcccc ctcaccataa
     3661 ggtgatggtc atcggcgcgc tcctgttcga cgtcgggtac gagccgcgtc gcataggagt
     3721 gatgagcgag tccaaggacg agccgggaat gctcggtgat ttcgcgaagt tactggagga
     3781 gcggcgcccc tgtctcgtca ctttcaacgg ccgcggcttc gacctgccgg tgatcgcgac
     3841 ccgctgcctc cgctacggca ttccgctccg ccactattac cggacgcgcg aggtgcggta
     3901 ccgcttctcg cccgagggcc acctcgacct gatggattac gtcgccgact tcggcgccgc
     3961 gaagccgtcg cgtctcgatg tcatcgccaa gctctgcggc atgccgggca aggtcggggt
     4021 ggatgggaaa gacgtcgggc cgctcgtcca cgcggggcgc atccaagagg tgcgtaacta
     4081 ttgcttgtgc gacgtggcgc agaccgccgg cgtcttcttg cgagtccagc tcctgcgcgg
     4141 cgagctcgag cgcgaaggtt acgtcgcggc gatgcgcaag ctcatcgagc tcatcaaagg
     4201 tgacgagcgc ctcgcgccgg tcgcgacggc gttgaacgag aagcggttgt tactcgaaga
     4261 aacgtaaacg gagggaacca tggatcgact cgtagacaag cgctgcacgg cttgcacccc
     4321 caacactcct cgcatcgagg caacggagct cccggcgtac ctagctcaag ctccagggtt
     4381 cggtgcgaac tccgaaaaga cgctgatcga gcggcgcttc gagttcaaaa actacccgga
     4441 gacgatgatc ttcgtgaacg cggtcgccgc catcgcgcac cgcgaggacc accaccccga
     4501 cctcgaggtg agctacggca gctgcaccgt gcgcttcacg acccacgcca tccacgggct
     4561 gagtgagaac gacttcatct gcgcggcgaa ggtgaacgcg ttgctcgcgt gacgctcaac
     4621 gcattccgcg agtgaactgt tcgccgaact tcacgaggtt gaagccgaag ctgaggccgc
     4681 cgtagcccat cgtttgcttt gcggagtcgc tcgtttcggc gaaccgcgcc gcgaagccgt
     4741 ccgcgcacgt ggcgaaccaa ccgaggtcca ggcacaagag accttgcacc caatcgctcg
     4801 cgccgccgct agtcgtgctg gcaatcaggt gatcggccga ggcgcggaac gaaacgccga
     4861 gcttcaagag catcaagttg aggaaaggtc cgcccttggc cccgagcccc atgcggcgca
     4921 ccgaatcgtt caggcggagc cgccccgcca ggaccggcgc gccatgggct cccacttcga
     4981 actgcaccgc ctcgctcccg taagcgtagc cgctctcgag caacggcagt gacagatgcg
     5041 atgccttgag cccacccaa
//
LOCUS       DASTKS010000727         6219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1903446, whole genome shotgun sequence.
ACCESSION   DASTKS010000727 DASTKS010000000
VERSION     DASTKS010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903446"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFQ35_15070"
     CDS             <1..515
                     /locus_tag="VFQ35_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932022.1"
                     /translation="IRVFHGVFGAFWVGSTLSAREDEAGRGSLQGRVVFVECRGIPVG
                     ESHPMRFAAPDAPTAPDRRAADTPMRFAAPDALRRARCASPRPMRFAAPAAPDRRAGC
                     ASPRRMRFAAPDALRRAGCASPRPMRPPRPMRFAAPDAPAGPMRFAASDAPAARRLKR
                     GDAGSEAGYG"
     gene            complement(524..1897)
                     /locus_tag="VFQ35_15075"
     CDS             complement(524..1897)
                     /locus_tag="VFQ35_15075"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HET9932023.1"
                     /translation="MYGRPRSRASELLVVASLGACACSLLAPPDENFLSPAHGGAGAD
                     AIVGGRASGGGRGGASTTGTTTGSTAGSSSDATAGGRGGAPASGGTGNTGSTGNAAGT
                     GSTVNPADPSGGVSGVGGQTSDGGGGTTSGVGGHASGGAQTSGGGGAPASGGVRTSEG
                     GTSPTNSQGGVGATSGALGAAGQTGSSPTCSTMTCGANAHCVQDDTGAHCVCDAASLP
                     DGLGCRLAFSCNELHQRAPTLPSGIYSIQPPSATRTAKVYCDMKESGGGWTLILNEGA
                     DFMPKTPGSDDAICYEKNCTSSVYATLPLASDILLDVADEDIIGANYAARIRILGVNS
                     ASRGKTLRDLFTSGPNFLERGDNGNLEIFTPHGEACEDVLPPDFASLVCDTCESAVAA
                     CGTPVITLGDADSACNDAVTFAIGASDSESESWTNCAGWPQAPFLVDSQSNQFQYYPT
                     NFRVWLR"
     gene            2255..3862
                     /locus_tag="VFQ35_15080"
     CDS             2255..3862
                     /locus_tag="VFQ35_15080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932024.1"
                     /translation="MVEPHSGWAMTFAQQRVIGRYLLGDAIAAGGMATVHFGRLLGPA
                     GFSRTVAIKQLRPGLPSDTESLTTFLDEARLASRIHHPNVVSPIDVVLIANEAFIVME
                     YVHGESLSRLLKAAPGVPVRPTLAAAVIGQVLFGLHAAHRAVSEDGVPLELVHRDVSP
                     QNILITPEGIARILDFGIAKAARQSHHTMNGVLKGKLAYMAPEQVTSSPVSSRTDIFA
                     AGTVLWEMLTGQRLFLAETPAACVQKILHDPIPAPSALVDDVPRELDEVVLRALARDP
                     EERFPDAQTMALAVAAAVRSASSLEVGAWVTELCGDSLLERGDRIADFEAMPLEELTL
                     GLPAASFGTPVPTGFTMANALLPPPAADALSSSRAARFRRRAAVFGAAVLTFGTLSVA
                     GPRAMQRFSQAGKSKVARTALANEPTPANEPTLAVASSESGSPHARRFVDSGSSSSTV
                     APSESPRPEGSRRSAVQTRLRPSVAAVGPSRSVDAPHTELARATPAVPNKLVSPKPVV
                     SAKRSPNCDIPYTIDERGVKRFNPDCL"
     gene            3862..4668
                     /locus_tag="VFQ35_15085"
     CDS             3862..4668
                     /locus_tag="VFQ35_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932025.1"
                     /translation="MPSVRSFRGLSTIAALVSVFGAAPARASDDGVRACVEAHAQGQV
                     DRGSGQLLAARRQFLSCSQETCPLVVRKECVAFAEQIAAETPSIVLVATDSRGHEVPL
                     ASAAIDASWSLNHDFGMALELDPGSHEIVLRALDGRAAQKTFLLRAGEHLRRVVVEFP
                     EPVPASPQEERPAAARGGVLPYVLGGVGLLALGSFVGFALDGKSQEHRLDECKPNCTR
                     EGVDRMRRSYLIADVSLAVALVAVGVDTYLVLKPPVSTQGAFGVSAMGRF"
     gene            complement(4674..5357)
                     /locus_tag="VFQ35_15090"
     CDS             complement(4674..5357)
                     /locus_tag="VFQ35_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932026.1"
                     /translation="MTFSRFWTPVRARSKPDVLPESVEADEPETGVLSLRQLMDSLGP
                     VDPAFDPDSTAHFSTDLLRRRSVQLASREPEPPSPSPEAPPPEPEPQLTSLQAGSPTE
                     RTGSRRLSPITLVSLVLLVPATALLFVPRGAPTVSPTSVEAPSQPRETPKLRAAADHH
                     EVHAPAPPALADEKGITRERSAVDHLAAGDFTRALEDYRAISGRSPAHAAAVRILGRK
                     LKAEQSATP"
     gene            complement(5354..>6219)
                     /locus_tag="VFQ35_15095"
     CDS             complement(5354..>6219)
                     /locus_tag="VFQ35_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9932027.1"
                     /translation="EARLPDGSRLQALVAPVAPGGPCVAIRRFSRDTFSLDRLVDLGT
                     LSHDAVATLDAFVVAKLNVLVAGGTGSGKTSLLNALTRFIPESERIVVIEDSKEVQVQ
                     HGHVVQLEAHPPDAEGRGAVSIRDLFRATLRLRPDRIVIGEIRGGEALDIVQAMVSGH
                     GGCLATLHATYPRDTLTRLETMALMSDVDIPLAALRAQIASGVNVIVQVARARDGARK
                     VTHITEVRGFDFATARYELADLFVRDVFTPKNELRPTGVMPTFLEQLAEHGARLPKSV
                     LDAGKRKEPKS"
BASE COUNT          949 a         2000 c         2127 g         1143 t
ORIGIN      
        1 tgattcgagt tttccatggc gtcttcggtg ctttctgggt cggttcgacc ctttctgcac
       61 gggaagacga ggcgggccgc ggttcattac agggacgggt cgttttcgtt gaatgccgcg
      121 gaattccggt gggcgagtcg cacccgatgc ggttcgccgc acccgatgcg cccaccgcgc
      181 ccgaccgccg cgccgccgac accccgatgc gcttcgccgc gcccgatgcg cttcgccgcg
      241 cccgatgcgc ttcgccgcgc ccgatgcgct tcgccgcgcc cgccgcgccc gaccgccgcg
      301 ccggatgcgc ttcgccgcgc cggatgcgct tcgccgcgcc ggatgcgctt cgccgcgccg
      361 gatgcgcttc gccgcgtccg atgcgcccgc cgcgtccgat gcgcttcgcc gcgcccgatg
      421 cgcccgccgg cccgatgcgc ttcgccgcgt ccgatgcgcc cgccgcacgg cgactgaagc
      481 gaggggacgc tggcagcgag gctggatatg gctagggcta gggctagcgc agccagacgc
      541 ggaagttggt cgggtagtat tggaactggt tcgattggct gtcgacgagg aagggggctt
      601 ggggccaccc tgcgcagttg gtccagcttt cggactcgct gtcgcttgct ccgatggcga
      661 aggtaacggc gtcgttgcac gctgaatcgg cgtctccgag cgtgatgacg ggggtgccgc
      721 acgcggctac ggcgctctcg caggtgtcgc aaacgagact cgcgaaatct gggggtaaga
      781 cgtcttcgca agcttcgccg tggggcgtga agatctctag gttgccgttg tcgccgcgtt
      841 cgaggaagtt gggtccggac gtgaataggt cccgcaaggt tttgcctcga gaagcggagt
      901 tgacgccgag gatgcgaatg cgagcagcgt agtttgcccc gatgatgtct tcgtcggcga
      961 cgtcgaggag gatgtcgctc gcgagaggga gggtggcgta gacgctcgag gtgcagttct
     1021 tttcgtagca aatcgcatca tcgctgcccg gcgtcttggg catgaagtcg gcgccttcgt
     1081 tcaagatcag tgtccacccg ccgcctgact ccttcatatc gcaatatact ttggcagtgc
     1141 gagtcgctga agggggttgg attgagtaga ttcccgaggg tagggtcggt gcgcgctgat
     1201 ggagttcgtt gcacgagaag gcgagccgac aaccgagacc atcgggcagc gacgccgcgt
     1261 cacaaacgca gtgggcgcca gtgtcgtctt gtacgcagtg agcattcgcg ccgcatgtca
     1321 tggtcgagca ggtcggcgac gatccggttt ggcccgcggc cccgagagcg ccgctcgtcg
     1381 ctccgacgcc gccttgggag ttggtcggcg acgttccgcc ttcgcttgtg cgcactccgc
     1441 ccgaagcggg cgcgccgcct ccacccgaag tttgggctcc acccgaagcg tggccgccga
     1501 ctcccgaagt ggtgcccccg ccgccgtccg aggtttgtcc ccccacgcct gagactccgc
     1561 ccgacgggtc cgcggggttt acggtgctgc ccgtgccggc ggcgttgccg gtgctgccgg
     1621 tgttgccggt gccgccactc gcgggggctc ctccacgtcc gcctgcggtc gcgtcggacg
     1681 acgagcccgc tgtcgaaccg gtcgtggtgc ctgtcgtcga cgcaccgcct ctcccgccgc
     1741 cgctcgctct tccgccaacg atagcgtcgg cgcctgctcc gccgtgtgcc ggactcaaaa
     1801 agttttcgtc cggtggagcg agcaagctac acgcgcacgc tccgagcgac gcaacgacga
     1861 gcagctcgct ggcgcgagaa cggggtcttc cgtacatgat gagtcttctt cgcgctactc
     1921 gagttcgaac gagggcgttt cgacgggagc ggccgctaaa gctacagtgt gcttacggcg
     1981 cacttcgggt gagcgcaaga cgcagaatcg aaacatgcgc ggcgctgcaa tccggtacta
     2041 acacccttcg cgtatatgcg gtaagctagc ggtgctcgaa cgctcgcgtg ggctcgcgcg
     2101 caccgagagt tcgcacaagg atacgttcgc gtcgttcgct gcagcggcgc gagctggcgg
     2161 caatcgccgc ttcaacttcg gaacgcgtgg acgtcatgga ttcgttgcac agctgtctgc
     2221 aatggtcgcg gccgcctgca tctaggctgc tggcatggtt gagccacatt ctggttgggc
     2281 catgacgttc gcgcagcagc gagtcattgg gcgctatttg ttgggcgacg ccatcgccgc
     2341 cggaggcatg gctaccgttc atttcggtcg tctgctcggg cccgctggtt tctcgcgaac
     2401 cgtcgcaatc aaacagctgc ggccggggtt gccgtcggac acggagtcgc tgacgacctt
     2461 tctcgacgag gcacgtctcg catcacgcat ccatcatccg aacgtcgttt ctcccatcga
     2521 cgtcgtgttg atcgccaacg aggcgttcat cgtgatggag tacgtgcacg gtgagtcgtt
     2581 gtcgcggctt ttgaaggctg cccctggcgt accagttcga ccgactttgg cagctgcggt
     2641 catcgggcaa gtgctgttcg gcctgcacgc agcgcatcgg gcggtctccg aagacggcgt
     2701 gcccctcgag ctcgtgcatc gcgacgtctc tccccagaac atcttgataa cgccagaggg
     2761 aatcgcgcgc attctcgatt ttggcatcgc gaaagcggca aggcagagcc accacaccat
     2821 gaacggtgtg ctcaagggca agctcgccta catggctccc gagcaagtga cctcgtcgcc
     2881 ggtcagcagc cgaacggaca tttttgcagc cggaacagtg ctttgggaaa tgctcacggg
     2941 ccagcgacta ttcctggctg agactcctgc agcttgcgtt caaaagatct tgcacgaccc
     3001 gatcccggcg ccgagtgcgc tcgtggacga cgtgccgaga gagctcgacg aggtcgtgct
     3061 gcgagcgctc gcgcgcgacc ctgaagagcg cttccccgac gcgcagacga tggcgcttgc
     3121 cgttgccgcg gcggtgcgtt cggcgagctc tctcgaggtg ggcgcctggg tcactgagct
     3181 ctgcggcgac tctctgctcg agcgcggtga tcgcatcgcc gacttcgagg cgatgccgct
     3241 cgaggagctg acccttgggc tcccggccgc gagcttcggc acacccgtcc cgacgggctt
     3301 cacgatggcc aacgccttgt tgccgcctcc agcggccgac gcgctgagca gctctcgagc
     3361 ggcgcggttt cgccggcgtg ctgccgtctt tggggcagcg gtgttgacgt tcgggacgct
     3421 gagcgtcgca gggccgcgtg ccatgcagcg cttttcccaa gccggcaagt ccaaagtagc
     3481 tcggaccgcg ctcgcgaacg agcccacgcc ggcgaacgaa cccacgctcg cggttgcgtc
     3541 ttcagaatcg gggtcgccgc atgcccgccg tttcgtcgac tcgggctcga gctcgtccac
     3601 agtagctcct tcagagtcgc cgcggcccga aggctcgcga aggagcgcgg tgcaaactcg
     3661 cctccgcccc tcggtggccg cggttggtcc ctctcgcagc gtcgacgctc cccatacgga
     3721 gcttgcgcgg gcgacgccgg ccgtgccaaa caagctcgtg tcgccgaagc ccgtggtctc
     3781 tgccaaacga tcacccaatt gcgatattcc ctacacgatc gacgagcgcg gagtgaagcg
     3841 cttcaacccg gattgcctgt gatgccaagc gttcgaagtt tccgcggcct ctccactatc
     3901 gcggcgttgg taagcgtctt tggcgcagcg cccgcgcgag ctagcgacga cggggtgcgc
     3961 gcctgcgtgg aggcccatgc acaaggccaa gtcgaccgcg gctccggtca gctgctcgcg
     4021 gcgcgccgac agttcctgtc gtgtagccaa gagacctgtc ccttggtcgt tcgcaaggag
     4081 tgcgtcgctt tcgccgaaca aatcgcggca gagactccct cgattgtttt ggtcgcgacc
     4141 gattcgcgcg ggcacgaggt gcccttggcg agcgcagcca tcgatgcgag ctggtcgctg
     4201 aaccacgatt ttggcatggc gctcgagctc gacccgggct cgcacgagat agttcttcga
     4261 gcgctcgacg ggcgcgccgc gcaaaagacg ttcctgctgc gcgcggggga gcacctgcgg
     4321 cgcgtcgtcg tcgaattccc agagccggtg ccggcttcgc cgcaagaaga gcgtcccgcg
     4381 gccgcgcgcg gaggggtact tccctacgtg ctcggcggcg tcgggttgct cgcgttgggg
     4441 tcgttcgtgg gttttgcgct cgacggaaag tcccaggagc atcggctcga cgagtgcaag
     4501 ccaaactgca ctcgcgaagg cgtcgaccga atgcgaaggt cgtacctcat cgccgacgtg
     4561 tcgctcgcgg tggcgttggt tgcggttggg gtcgacacct atttggtgtt gaagccgccg
     4621 gtctcgacgc agggagcgtt cggcgtcagc gcgatgggaa ggttctgagt cgttcacggt
     4681 gtggcgctct gttctgcctt gagcttccgt ccgagaatgc gcacggcggc cgcgtgcgcg
     4741 ggcgagcgcc cggaaatcgc ccggtagtct tcgagggctc gcgtgaagtc gcctgccgcg
     4801 aggtggtcga cggcggagcg ttcccgcgtg atccctttct cgtcggcgag ggctggcggc
     4861 gcaggcgcgt gcacctcgtg gtggtccgcc gccgcgcgca actttggagt ctctcgcggt
     4921 tgcgacggag cctccacgct agttggtgaa acagtaggcg caccgcgagg cacgaagagc
     4981 aacgccgtcg ccggaaccag caggactaac gaaaccaggg tgatcggtga tagccggcgc
     5041 gagccggttc gctcggtcgg ggaacccgct tgcaggctgg ttagttgcgg ttcgggttcg
     5101 ggtggaggcg cttcgggcga aggcgacggc ggctccggtt cgcgcgaagc gagctgaacg
     5161 ctgcggcggc gcagcaagtc ggtcgaaaag tgcgcggtgg agtcagggtc gaaggcaggg
     5221 tcgacaggcc caaggctatc catcaattga cgaagcgaga gcacgcccgt ttctggctcg
     5281 tccgcttcga ccgactctgg cagcacgtcg ggtttcgatc gcgcgcgcac cggcgtccag
     5341 aatcgcgaga acgtcacgac ttcggctcct ttcgtttgcc cgcatcgagc acgctcttcg
     5401 ggagccgcgc gccgtgctcg gcgagttgct cgaggaacgt tggcatgact cccgtcgggc
     5461 gaagctcgtt cttgggggtg aacacgtcgc gcacgaacag atcggcgagc tcgtagcggg
     5521 ccgtggcgaa gtcgaacccg cgcacttcgg tgatgtgggt gaccttgcgg gcaccgtcgc
     5581 gcgctcgtgc gacttgaacg atgacgttca cgccggaggc gatttgggcg cgcaaggccg
     5641 cgagcgggat gtcgacgtcg ctcatcaagg ccatcgtttc gagccgcgtc aacgtgtcgc
     5701 gcgggtacgt ggcgtgcagc gtggccaagc aaccgccgtg gccgctgacc atcgcctgca
     5761 cgatgtcgag cgcctcgcca ccgcggattt ccccgatcac gatgcggtcc gggcgcaagc
     5821 ggagcgtcgc tcggaagagg tcgcggatcg acaccgctcc acggccttcc gcgtcgggcg
     5881 ggtgcgcctc gagctgcacg acgtgtccat gctggacctg cacttccttc gaatcttcga
     5941 tgacgacgat gcgctcgctc tccggaatga agcgagtgag ggcgttgagc aacgaagttt
     6001 ttccgctccc agtgcccccg gcgacgagca cgttgagctt ggcgactacg aaagcgtcca
     6061 gcgtggcgac ggcgtcgtga ctcagcgtcc cgaggtctac caatcgatcc aggctgaagg
     6121 tgtcccgaga gaaccggcga atggcgacgc agggcccgcc gggggccacg ggcgccacga
     6181 gcgcttgcag acgcgagccg tcgggcaacc gggcttcca
//
LOCUS       DASTKS010000728         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_339999, whole genome shotgun sequence.
ACCESSION   DASTKS010000728 DASTKS010000000
VERSION     DASTKS010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339999"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..1381
                     /locus_tag="VFQ35_15100"
     CDS             404..1381
                     /locus_tag="VFQ35_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932028.1"
                     /translation="MKARVSWWGACEDGEALKRELGERGVLLDEPTEGDKDAVSLTIA
                     VQPTPASAWNARLIVADASGREHERVIQVKSCDELRAALAWVLLVLAREGVVPSQESV
                     LSSAAGFSSGSEGDSNAGAQPSVASAPPTPPPRVEPPPKSPSPTPTTVAVPRSARGVL
                     RVGTLFLGAFGLLPKGALGPALYAEYEPPARGVPAFSVALFQLTTLAYERGGVTLDVA
                     RVGGRLGARIGTGWEPLSVALALEAGRLTGSGTGALLASRHDSELWVAAELGPTLRAP
                     LIGRVLAGELGGALAYTPFRYSFQFGDGASLSRSENFEVRLFAGLSAKL"
     gene            1449..2024
                     /locus_tag="VFQ35_15105"
     CDS             1449..2024
                     /locus_tag="VFQ35_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932029.1"
                     /translation="MVRHEAEAVLLEAAAEPPAVLAAARVRDIVERDYALVWRFLRRL
                     GVPESEVDDLAQAVFARVLARAEVIVPGAERAYLMKAAYHASFEGRRALSRARGRAAA
                     IDPEELQSHEPLPDQSLHARQQRELLDAALARLTPELRAVFTLFELEELTFSEIAELL
                     DIPRGTVASRLRKARQVFTEAVKRLEPGVLE"
     gene            2021..>2355
                     /locus_tag="VFQ35_15110"
     CDS             2021..>2355
                     /locus_tag="VFQ35_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932030.1"
                     /translation="MTDDLEPPRLIDAGDPELRSLLESARHDAPTNRALKAAPFAIGA
                     LLASGAAEAATVATAGGVTIAAKWIAIGLVAGSVASAGAVKVQSAMQAPSSAVSAVTA
                     ARAPQRKRPP"
BASE COUNT          346 a          745 c          850 g          414 t
ORIGIN      
        1 cgtcgtgacc gccgaggcag gcaagctagc cccgctgcca agcacgtagc ttcgcttcgc
       61 gctcagcgcc gaggccggca gtgtcgcgcg cgacgcggct gaacgcattc gacgtgagtt
      121 tctcgactgg gggagtagcc gagcggagct cgcggcgaat tccgttgcga gccgtccgaa
      181 gcgtttggcg tccgcgccgt cgccgagccg aagtagcggc gctcttttta ggtgtggaaa
      241 ttccgcgaaa atgagggagc acccaggggt cgcgcgcgcg gctggccttc gtgttagggt
      301 gcgtcgttct tcgcgcatga agatcgtcgg tcgcttaggt gttggggttc tcttagcgtc
      361 gcagctgctc ctcggagcgc aatccgcgcg ggcagagtca cgagtgaagg cgcgcgtttc
      421 gtggtgggga gcgtgcgagg acggcgaggc gttgaagcgg gagctcggtg agcgcggagt
      481 tctgctggac gagccgacgg agggcgacaa ggacgcagtt tctctgacga tcgccgtaca
      541 gccgacgccg gcgtcggctt ggaacgcgcg gctcatcgtc gctgatgcca gcggtcgcga
      601 gcacgagcga gtgattcagg tgaagagctg cgacgaattg cgggcagcgc ttgcctgggt
      661 gctgctggtg ctggcgcgcg aaggcgtggt gccgagtcag gagagcgtgc tgagctcggc
      721 ggccgggttc tcgtccggtt ccgagggcga ttcgaacgcg ggcgcgcagc caagtgttgc
      781 gagcgcgccg ccgaccccac cgccgcgcgt ggaacctcct ccgaagtctc cttctcccac
      841 tcccacgacc gtcgcagtgc cgcggagtgc ccgtggcgtg ttgcgcgtgg gtacgctgtt
      901 cctgggagcg tttggcttgc tgcccaaggg ggcgctcggg ccagcgctgt acgcggaata
      961 cgagcctcct gcgcgcggag ttccagcttt cagcgtggcg ctcttccaat tgacgacgct
     1021 cgcgtacgag cgaggcggcg taaccttgga tgtggcgcgc gtcggcgggc ggctgggtgc
     1081 gcgcattggc acgggttggg agccattgtc ggtggcgctc gcgctcgagg cggggcggct
     1141 taccgggtcg ggtacgggcg cgctgctggc ctcgcgacac gattccgagc tgtgggtggc
     1201 cgcggagctc gggcccacgt tgcgcgcacc gctcatcggc cgagtcttgg cgggggagtt
     1261 ggggggagcc ctggcctaca cgccgtttcg ctattctttc cagtttggcg acggggcctc
     1321 gctgtcccgg tccgagaatt tcgaggtgcg gctgttcgcc ggactttcgg ccaagctcta
     1381 gctcgaaaaa gctgtcactt ttcgtgatcc aaaggctgcc gtccgagctt tgaagtcggt
     1441 ggacctgcat ggtgcgacac gaggccgaag ccgtgctgct cgaagcggca gctgagcccc
     1501 ccgccgtttt ggcggcggcg cgcgtgcgcg acatcgtcga acgagactac gcgctcgtgt
     1561 ggcgcttctt gcgtcgcctc ggcgtgcccg agagcgaggt agacgacctg gcgcaagccg
     1621 tctttgcgcg cgtgctcgct cgagccgaag tgatagttcc gggcgccgag cgcgcttact
     1681 tgatgaaggc ggcttaccac gcctcgttcg aaggacgccg ggcactcagc cgggcgcggg
     1741 ggcgcgctgc cgccatcgat cccgaagagc ttcaaagcca cgagccgctt ccggaccaat
     1801 cgttgcacgc gcgacaacag cgcgagctgc tcgatgcggc gcttgcgcgc ctcacacccg
     1861 agcttcgcgc ggtgttcacg ctgttcgaac tcgaggagct cactttcagc gagattgcgg
     1921 agctgctcga cataccgcgc ggcaccgtcg cgtcgcgcct gcgcaaagcg cgccaagtgt
     1981 tcaccgaagc cgtcaaacgg ctcgaacccg gagtcctcga atgacggacg acctcgaacc
     2041 gcctcgcttg attgacgcgg gcgatccgga gctgcgctca ctactcgagt cagcgcgcca
     2101 cgacgccccg accaaccggg ccctcaaagc tgcacccttc gcgatcggcg cattgctcgc
     2161 gtcgggagcg gccgaagcgg cgacggttgc tacggcaggt ggcgtcacga tcgccgcgaa
     2221 atggatagcc atcggactcg tggctgggtc ggtcgcttcg gccggtgcgg tcaaagtgca
     2281 gagcgccatg caagcgccct ccagcgcggt ctcggccgtc acggcggctc gcgccccgca
     2341 gcggaaaagg ccgcc
//
LOCUS       DASTKS010000729         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1456995, whole genome shotgun sequence.
ACCESSION   DASTKS010000729 DASTKS010000000
VERSION     DASTKS010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456995"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1582)
                     /locus_tag="VFQ35_15115"
     CDS             complement(68..1582)
                     /locus_tag="VFQ35_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9932031.1"
                     /translation="MGAFKEFSRFDAVGLGELVRRREVTPLELCDEAIARIERLNPEL
                     NAVVVKLYDSARERAERPFDMTSPVAGVPFLVKDLLSPVAGAPMSSGSRLYRNHVPDY
                     DAELVRRYRSAGLVLVGKTNTPEFGILPITEPVLFGACKNPWDLQRTPGGSSGGAAAA
                     VASGIVPVAHGGDGGGSLRIPASCCGLFGLKPSRGRNPVGPDASEHWLGLAVEHVVSR
                     SVRDSAALLDVSAGPEATSPYHAPPQVRPYVEELRQRPAKLRIAVSERPHLTGKVHPD
                     CRRALSDAATLCRDLGHEVEEASIDIDPDEFGHAFFTVICGSVAAGIERAAVELGRRP
                     ARDELEVATWLAGLLGAELSAGAAIAGLEVLQQVARRALRFFERYDVLLTPTLSKPPL
                     FIGALEPRGAEALAHKTIANLGIGSILRLKRVIKATVDRVFDFVPFTPLANVTGQPSM
                     SVPLYWNADGLPIGSLFTARLGDEATLFRLAAQLEEARPWAQRKPSIHADSLRS"
     gene            complement(1802..2743)
                     /locus_tag="VFQ35_15120"
     CDS             complement(1802..2743)
                     /locus_tag="VFQ35_15120"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HET9932032.1"
                     /translation="MTRSVRSLFGVWAGFLVALCCIAVSLVARAEPSEPAGATSAEAA
                     GPPPEWQPGPRTVNLGHDIKVELPADYMYLPPAEAKKALEKGGSFHNDAVLGLLASVK
                     PSDDWFVVLQYEDSGYVKDDEKLDADELLSSMREAVKDSNEERVKRGFKPLALDGWSE
                     PPRYEKAQHHMVWALIVSDPDGKSVNFNTRVLGRRGYVSLNLVTAPESLATYKSNATT
                     LLAATHFDSGARYEDFNEKTDKVAEYGLTGLILAGAGLGAAKLVKIGLLAKFWKVLIG
                     IIIAGKKAIVLAVLAIGGAIKKFFGSKGNQETPSASG"
     gene            complement(2856..>4197)
                     /locus_tag="VFQ35_15125"
     CDS             complement(2856..>4197)
                     /locus_tag="VFQ35_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9932033.1"
                     /translation="PQRARASERRKRDLVGFLDYYREHRKGGRSWRLVIAGDFVDFVG
                     MSVLPTADYETEPSEEELAHGLGGAWDHALAKLRLLMRHEVEVMTSLARFIADGNSLV
                     IVRGNHDVEWHWEAVQKAFRDELSALANFAPEQLEFRAWFYYEEGRVFIEHGHQYDAY
                     CSFEHILNPVSPADPRRTVRSLSDVLVRYVVRPTRGMTEAGHAAAGIWDYLRFAASLG
                     FRGMLQLTRRFAASTLALFSLWREHVSEAAAHIQHEQELRLSALGRAHKIQVERLKAL
                     LRLHRPPITKSLPAIFASVMLDRVLLAFVGLAMLFAIAMWAPSWRHGLLEAGAATVAL
                     LGIGYAWRRMRDTLEPSAELRERSPLIARLFPAALVVMGHTHLPEKHVANDSTTYVNL
                     GAWAEDDVEDGHTPNLPATRTHLVVADAGDESVTELFTWHDEGPAVFRPEPRSP"
BASE COUNT          733 a         1391 c         1358 g          715 t
ORIGIN      
        1 cggcgagggg cagaacttgg cccccccgag aaagccgccc gaggcccctg tcggtttgcg
       61 cgggcgctca gctcctcagc gaatctgcgt gaatggaggg tttgcgttgc gcccagggcc
      121 gcgcctcttc gagctgagcg gcgagtcgaa acagcgtcgc ttcgtcgccg agccgggccg
      181 tgaagaggga accgatgggc agcccgtccg cgttccaata caacggcacg ctcatcgagg
      241 gttgcccggt cacgttggcg agcggtgtga acgggacgaa gtcgaacact cgatccacgg
      301 tcgccttgat cacccgcttc agtcgcaaga tcgagccgat gccgaggttc gcgatggtct
      361 tgtgagcgag cgcctcggcc ccgcgcggtt cgagcgctcc gatgaagagc ggcggcttcg
      421 agagcgtcgg cgtgagcagc acgtcgtagc gctcgaagaa gcgcagcgcg cgtcgcgcca
      481 cctgctgcag cacctcgagc ccggcgatgg cggcccccgc cgaaagctct gcgccgagca
      541 gccccgcgag ccaagtcgct acttcgagct cgtcgcgagc cggccggcgc ccgagctcga
      601 ctgcggcgcg ctcgatgccc gccgcgactg aaccgcagat caccgtgaag aaggcgtgcc
      661 cgaactcgtc cggatcgatg tcgatgctcg cttcctccac ctcgtgaccg aggtcacggc
      721 agagcgtcgc cgcgtccgag agcgctcgac ggcagtcggg gtgcaccttg ccggttagat
      781 gcgggcgctc gctcaccgca atgcgaagct ttgcggggcg ctggcggagc tcctcgacgt
      841 acgggcgaac ctgcggaggc gcatggtaag gcgacgtcgc ttcggggccc gcgctcacgt
      901 cgagcaacgc ggcactgtcg cgaacactcc gcgagaccac gtgctcgacg gcgagcccga
      961 gccaatgctc gctcgcgtct ggacccaccg ggttgcgtcc gcggctcggc ttcaagccga
     1021 acaggccaca acaagacgcc ggaatacgca gcgacccccc accgtccccg ccgtgcgcca
     1081 ccggtacgat gccggatgct acggcggccg cagctccacc gctcgagccg cccggtgtac
     1141 gctggaggtc ccacggattc ttacaggcgc cgaagaggac cggttctgta atcggcaaga
     1201 tgccgaactc cggcgtattc gtctttccga ccaagacgag ccctgcgctg cgatagcgcc
     1261 tgacgagctc ggcgtcgtag tcgggcacgt ggttgcgata caaccggctt ccggagctca
     1321 tgggtgctcc tgccaccggc gatagcaagt ccttaaccaa gaacggcacg cccgccacgg
     1381 gactcgtcat gtcgaacggg cgctcggcgc gttcgcgcgc gctgtcgtac agcttgacga
     1441 cgacagcgtt caactccgga ttcagtcgct cgattcgagc gatggcttcg tcgcagagct
     1501 cgagcggagt cacctcccgg cggcgtacca actcgccgag accgacggcg tcgaatctgg
     1561 aaaactcctt gaatgcgccc atggaacgac tctcgccgcg tacgtcatgc ggttgagtcc
     1621 gccgtcgaac gcccacggcg tcacccaatc gagccccgag tgcaggcgca agccgcgcct
     1681 gaccgaggcc gcacgctcta acgctttcga gtatagcgct gctttttaaa atcgcccgcc
     1741 gcgagagtcg tcagtgtggc gggcgatcac cggggagcgt tccgagctac gtacgtgagc
     1801 ttcagccgga ggcgctcggc gtctcttggt tgcccttgct gccgaagaat ttcttgatgg
     1861 cgccaccaat ggcgagcacc gcgagcacga tcgctttctt accggcgatg atgatgccga
     1921 tcaaaacctt ccaaaacttc gccaagaggc cgattttgac gagctttgcc gcgcccaagc
     1981 cagcgcccgc gaggatgagg ccggtgaggc cgtactcggc gactttgtcg gtcttctcgt
     2041 tgaaatcttc gtagcgcgcg ccgctgtcga agtgagtggc ggcgagcagc gtcgtggcat
     2101 tcgacttgta ggtggcgagg ctctcgggtg cggtaaccag gttcaaagag acatagccgc
     2161 ggcgtccgag gacacgggtg ttgaagttca ccgatttacc atccgggtcg ctcacgatga
     2221 gagcccacac catgtggtgt tgcgccttct cgtaacgcgg cggttccgac cagccgtcga
     2281 gcgccagcgg cttgaagccc cgcttgacgc gctcttcgtt cgaatccttc acggcctcgc
     2341 gcatggatga gagcagctca tcggcgtcga gcttttcgtc gtccttcacg tagccggagt
     2401 cctcgtactg aaggacgacg aaccagtcgt cgctcggctt cacgctggcg agcagtccga
     2461 gcacggcgtc gttgtggaaa ctcccaccct tctcgagcgc cttcttcgcc tcggcaggcg
     2521 gcaagtacat gtagtcggcg ggaagttcga ccttgatgtc gtgaccaagg ttcacggttc
     2581 gggggcccgg ttgccactct ggaggaggtc ccgcagcttc ggcggacgtg gcgcccgccg
     2641 gctcggaagg ttcggcgcgc gcgaccagcg agaccgcgat gcagcacagc gccaccaaga
     2701 aaccagccca gaccccaaac agcgacctaa cagagcgcgt cattttgcat gccttctttg
     2761 atcccgagcc ccacctgaac ccggctcgga aggagcagct tatcgcgctc gctgtttctc
     2821 tggaagtcgc aacccgctct ggaagctcta cgaagtcacg gcgatctcgg ctcgggacga
     2881 aacacggctg gcccctcgtc gtgccaggtg aaaagctcgg tgaccgattc gtcccccgca
     2941 tctgcaacta ctaggtgcgt gcgagtggca ggcaggtttg gcgtgtgccc atcttcgacg
     3001 tcgtcctcgg cccaggcgcc gaggttcacg taggtcgtgc tgtcgttcgc gacgtgcttc
     3061 tcgggaaggt gggtgtggcc catcacgacc aaggcagcgg ggaacagccg cgcgatcagt
     3121 ggcgagcgct cacgcagctc ggcgctcggc tcgagggtgt cgcgcattct gcgccacgcg
     3181 tatccaattc cgagcagcgc gaccgttgcg gcccccgcct cgagcaggcc gtgccgccac
     3241 gaaggcgccc acatcgcgat cgcgaagagc atcgcgaggc caacgaaagc caacaacact
     3301 cgatcgagca tcacgctcgc gaagatggcg gggaggctct tcgtaatcgg cggccgatga
     3361 agccgcagca atgctttcag tcgctccacc tgaatcttgt gggcacgacc gagcgcgctc
     3421 aagcggagct cttgctcgtg ctggatgtgc gccgcggctt cgctcacgtg ctcgcgccaa
     3481 agagagaaca gcgcgagggt gctcgcggcg aagcggcgcg tgagctgcaa catgccgcga
     3541 aacccgaggc tcgccgcgaa gcggaggtag tcccaaatgc cggcggcggc gtgcccagct
     3601 tcggtcatgc cgcgcgtcgg tcgcacgacg tagcgcacga gcacgtccga tagcgagcgc
     3661 acggtgcgcc gcggatctgc aggcgagacc gggttcaaga tgtgctcgaa gctgcagtaa
     3721 gcgtcgtact gatgcccgtg ttcgatgaag acgcgcccct cttcgtagta gaaccaggcg
     3781 cggaactcga gctgctccgg tgcgaagttc gcgagcgcgc tgagctcgtc gcgaaaggct
     3841 ttctgcaccg cttcccagtg ccactcgacg tcgtggttcc ctcgcacgat gacgagcgag
     3901 ttgccgtcgg cgatgaagcg agcaaggctc gtcatcacct cgacctcgtg ccgcatcagc
     3961 agtcggagct tggcaagagc atggtcccag gccccgccga ggccgtgcgc cagctcttct
     4021 tcgctgggct cggtctcgta atcggccgtc ggcagcacgc tcatccccac gaagtcgacg
     4081 aaatcgccgg cgatgacgag ccgccaggag cgcccgccct tgcgatgctc tcgatagtaa
     4141 tcgagaaagc cgaccaggtc gcgttttcgc cgctcactcg cccgtgcgcg ttggggc
//
LOCUS       DASTKS010000730         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1903841, whole genome shotgun sequence.
ACCESSION   DASTKS010000730 DASTKS010000000
VERSION     DASTKS010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903841"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1385
                     /locus_tag="VFQ35_15130"
     CDS             237..1385
                     /locus_tag="VFQ35_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932034.1"
                     /translation="MNMRSLHQLSQIALFASLVAACSSKSETDAELEGVPEQAAFALS
                     VTDDATSEGSATADDAVDPSADITSSLEAVSQAVESDVAPELANARGAVRALNQALRH
                     FMQPIVALVRDTEPTSTVGRVKVWGPVTRGPTEFRFTLRHGTLRHYGWVLEARPEGSN
                     DAFTTVAAGGITVGFAPRRGRGTVGIDLDALASVDPTVVAGGALLARFAHGAAGSIVG
                     YRLRDFMSEAQATPINALVQGVHLKGGTNRLRLAYHGNLPETATAAPELVLARVRHQR
                     GEGGRADLLVTGGDVPNGHAWVVSECWNSTLKAGFRRVLDCPGDGIGGERCEVVATTG
                     DASACRALFAQAELPPADAEAPMSDDASPEGDVTPPEAMPDGAPPADG"
     gene            1604..2035
                     /locus_tag="VFQ35_15135"
     CDS             1604..2035
                     /locus_tag="VFQ35_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932035.1"
                     /translation="MACKSQHLWRSLLVVGIVGMAGCRASYVDRGAALYAEHNYLEAD
                     ELFEHNEARLQSAPVAERARYALYRGVTLLSLGDSARSRYWLSYAARCGERDSQALSS
                     DERSMLTHALNTHSEYEARIASRRRAVEGARASLESAPSAQ"
     gene            2198..3136
                     /locus_tag="VFQ35_15140"
     CDS             2198..3136
                     /locus_tag="VFQ35_15140"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9932036.1"
                     /translation="MILKSVRVSAIAARSMWACVLLAPIGCGQAPLEPREPTPGRAHF
                     TIATFNVEDGKQTDQATVEAIGATKADLICLQETGDGWIEVIKARYAAEYPYMMFEPG
                     GTGGLAVLSKQRLVSRAVYPGEAGGHPSWSLFAETPAGWLSLLNVHLRSLYAAKKGLI
                     GDYLSWGSDHVTQLESVWSNSSTGEEVVRTMPRLVMGDFNDGENGDAVDYLTSIGLTD
                     VLPLFHPGQYTWRHPSLAGQFNEALDHVLFDSWVEPLDAYVLNRGNSDHIPVVAHFEA
                     SAPWPEFDPSKLQPGTIDPHTIAGTQPVITLESTQN"
     gene            complement(3127..>3674)
                     /locus_tag="VFQ35_15145"
     CDS             complement(3127..>3674)
                     /locus_tag="VFQ35_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932037.1"
                     /translation="LGSVVALSFGCAPVAMMRPPGVIGDDRSFEAGSAVASLGKRPYV
                     EESAQYTGQAWFSADVKRWLTLSGIAAFDTSAAAVGGAARWNALRTGRFTVGPELEFG
                     YAWTAASLGGSVRVIGRNYVYTAPRFGTMGSHWSVGVPAGVSIDLTHGFALRAEGQLS
                     WQELKYYNRRLHLAAGLAYQF"
BASE COUNT          681 a         1193 c         1204 g          596 t
ORIGIN      
        1 tctcaagatt cgcgcgcgcc tcgccggtcg cagcgcgctc tatcgtgacc tcaatgacaa
       61 cggcgtgctc gacgccgacg aacgcagcgg cgagctgatg gtcggcgacg aaaacgccga
      121 cgagtagctc gcaatactcg aaatcacgcg cccgcgtcga cgcggagcct cgaggctccg
      181 cgaacggctc ggtgttgccg aaaacccaaa gaaagtccga acgagaagga aacatcatga
      241 acatgcgctc tcttcatcag ttgtcccaaa tcgctctctt tgcgtcgctc gtcgcggcgt
      301 gcagctcgaa gtcggaaacc gacgcggagc tcgaaggggt gccggagcaa gcggcgttcg
      361 cgctcagcgt caccgacgac gccacgagcg aaggctccgc gaccgccgac gacgccgtcg
      421 atccgagcgc ggacatcacg agctcgctcg aagcggtctc tcaagccgta gagagcgacg
      481 tcgctcccga gctcgcgaac gcgcgcggcg cggtgcgcgc gttgaaccaa gcgctgcgcc
      541 acttcatgca gccaatcgtt gcgctcgtgc gcgacaccga gccgacgtcg acggtcggtc
      601 gagtgaaagt gtgggggccg gtgacgcgcg gcccgaccga gttccgtttc acgctgcggc
      661 acggcacact gcgtcactac ggttgggtgc tcgaagcgcg tccggagggg agcaacgacg
      721 ccttcacgac cgtcgcggcc ggcggcatca cggtgggctt cgctccgcgt cgcgggcgcg
      781 ggaccgtggg catcgatttg gatgcgctcg cgagcgtcga ccccaccgtc gtagcgggcg
      841 gcgcgctgct cgctcgcttc gcgcacggcg cggcgggctc gatagtcggg tatcgactcc
      901 gagacttcat gagcgaggcg caagcgacgc caatcaatgc gctggtccaa ggcgttcacc
      961 tgaagggcgg aaccaatcgc ttacgcctcg cgtaccacgg gaacctccca gagacggcca
     1021 ccgcggcccc tgagctcgtg ctggcgcgcg tcaggcacca gcgcggcgaa ggcggtcgcg
     1081 cggatctgtt ggtgacgggc ggtgacgtgc cgaacgggca cgcttgggtg gtgagcgagt
     1141 gctggaactc gacgctgaaa gcgggtttcc gacgtgtgct cgactgccct ggcgacggca
     1201 tcggaggcga gcgttgcgaa gtggtcgcga cgactggcga cgcgagcgcc tgccgcgcgc
     1261 tctttgctca agccgagttg cctccggcgg acgcagaagc tccgatgtcg gacgacgcga
     1321 gccccgaagg tgacgtgacg ccacccgaag cgatgcccga cggcgcgcct cccgccgatg
     1381 gttgaacgcg gctttctgcg acacggcccg ctcatcggtt ggagcgggcc gcgcctgcgt
     1441 acgctcgagt catagaaatc gcgctcgagc acgagtgcct cagtttgaca gcgccgcctg
     1501 cgacaattga ccactcagcc gctgcggctc gcgaaaacag gccggatctc gctcggcatt
     1561 gcccttgcaa actccactta tataccgctt tggagattgc aaaatggcct gcaaaagcca
     1621 gcatttgtgg cgctcgttgc tcgttgtcgg aatcgtcgga atggcagggt gccgagcgtc
     1681 ctacgtggac cgcggcgcgg ccctctacgc ggagcacaac tacctcgaag ccgacgagct
     1741 cttcgaacac aacgaggccc ggctccaaag cgccccagtc gccgagcgag cgcgctatgc
     1801 actgtatcgc ggagtgacgc tgctctcgct cggtgactcc gcgcgctcga gatactggtt
     1861 gagttacgcc gctcgctgcg gcgagcgaga ttctcaagcg ctgagctccg acgagcgctc
     1921 gatgctcacg cacgcgctga atacgcactc tgagtacgag gcgcgcatcg cgagtaggcg
     1981 acgcgcggtc gagggtgcgc gcgcgtcgct cgagtcggca ccgagcgcgc agtaacgcga
     2041 atgcgaattc gtttcacact gacgctcgcg tcagcgaatg cgacagaaga actctctcgg
     2101 cgataaaatg cgtcggagct cgaaagcgac gcgtgcaagc gcgcttatga cgaactccct
     2161 tgcgcccctg tgactccaag tcacactgcc tacgccgatg atcctgaaga gcgtaagggt
     2221 gagcgcgatc gctgcgcgct cgatgtgggc ctgtgtctta ctagcgccga ttggctgcgg
     2281 acaggctccg ctcgagccac gcgagccaac gccgggccgc gcgcacttca cgattgcgac
     2341 gttcaacgtc gaagacggca aacagaccga ccaagccacg gtcgaggcga tcggcgccac
     2401 caaggccgat ctgatttgcc ttcaagagac cggcgacggc tggatcgagg tcatcaaagc
     2461 acgttacgcc gctgaatatc catacatgat gttcgagccg ggcggcacgg gcgggctcgc
     2521 ggtgctttcg aagcagcgct tggtgagccg cgcggtgtat cccggcgagg ccggtggtca
     2581 tccgagctgg tcgctcttcg cggagactcc ggcgggctgg ctcagcttgc tcaacgtcca
     2641 cctgcgttcg ctctacgccg ccaagaaggg gctcatcggc gactatcttt cgtggggctc
     2701 ggaccacgtt acgcagctcg aatcggtgtg gtcgaacagc agcactggcg aggaagtggt
     2761 tcgaacgatg ccccgcctgg tgatgggcga tttcaacgat ggtgagaacg gtgacgccgt
     2821 cgactacctg acgtcgattg gactaaccga cgtgttgccg ctgttccacc ccgggcagta
     2881 cacgtggcgt cacccttcgc tcgccgggca attcaacgaa gcgctcgatc acgtgctctt
     2941 cgacagctgg gtcgagccgc tcgacgccta cgtgctgaat cggggcaact cggatcatat
     3001 cccggtggtc gcgcatttcg aagcgagcgc gccgtggcct gaattcgatc cctcgaagct
     3061 ccaaccggga acgatcgatc cgcatacgat cgcggggacg caaccggtga tcacccttga
     3121 aagcactcag aactgatagg cgagccccgc cgcaaggtgc agcctgcgat tgtagtactt
     3181 gagctcttgc cagctgagct gtccttcggc gcgcagcgcg aagccgtgag tcaaatcgat
     3241 gctgacgcca gcgggaacac caaccgacca gtgtgagccc atggtgccaa agcgcggcgc
     3301 cgtgtaaacg tagttccggc cgatgacgcg gacgctgccg ccgagcgaag ccgcggtcca
     3361 ggcgtagccg aactcgagct ccggcccgac cgtgaaccgg cccgtgcgca gcgcattcca
     3421 acgcgcggcg ccgccaacgg ctgcggcgga cgtgtcgaat gcggcgatgc cagaaagagt
     3481 gagccagcgc ttcacgtcgg cgctgaacca cgcttgcccg gtgtactgag ccgactcctc
     3541 gacgtacggc cgtttgccga gcgacgcgac ggcgctgcct gcttcgaagc tgcgatcgtc
     3601 tccgatgaca cccggtggcc gcatcatcgc gactggcgcg cagccgaagg aaagggcgac
     3661 gaccgaacca agac
//
LOCUS       DASTKS010000731         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1457115, whole genome shotgun sequence.
ACCESSION   DASTKS010000731 DASTKS010000000
VERSION     DASTKS010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457115"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFQ35_15150"
     CDS             complement(<1..183)
                     /locus_tag="VFQ35_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932038.1"
                     /translation="MGARSAEARGAARFRDFATGTAEVAGTAAGATADAGGDTDPASG
                     GAGEVTSAAAAGPTAAR"
     gene            279..1754
                     /locus_tag="VFQ35_15155"
     CDS             279..1754
                     /locus_tag="VFQ35_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9932039.1"
                     /translation="MVEGVRPVLFGFLLGVLATAHARADEPETDASAQAADAATQSAR
                     RHFRVGVKLYRDANYPGALAEFEAAYRDKPGPGSLQNVALSLKALFRYAEAASTLRLL
                     LERHATELSDSERAAMQTALAELESLVGTLVIAIQPAEASVTIDGRATTRDDISRGVS
                     LNVGEHTVVVEARGYERRAQIVRIASLQRLALNVDLKPTAGFIHVVASDPKAAIAIDD
                     EPLAYHTYRGPLTPDVEHVVQVYRAGFEPFEKMVRVELGRTVDVQAELGAPTGVVDDR
                     AELPKKPVAPPPSRKPVGYYALATVAAVGLNDTPLNLRVDGSSGITLPSFGLRGGYRL
                     SEPVAVELAVDFGRLEAKDACELDQSDTCVTKRDFSLRSFRFGPNLRLMTHGEVVRFA
                     TSVGAGLVSHTLSLSRDEASARAGGSASGVDPYFSLELGAAFNYRHLLAEVGAIAFIE
                     GATGLHGAFDEQTERAVFSSGTLPMLGFVLKLGYSAWSPRN"
     gene            1897..>3141
                     /locus_tag="VFQ35_15160"
     CDS             1897..>3141
                     /locus_tag="VFQ35_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9932040.1"
                     /translation="MSHDTTAIESHSSETRTFEPSPEFAQLARIRSAEEYQKLYRESL
                     DSPETFWKRETSELVFRTPWKQLLDWKLPHAKWFLGATLNVTESCLDKHLSGARRDKT
                     AIIWESEPGESKKLTYAELHREVVELSAALRGLGIKKGDRVAIYMGMVPEVVVGMLAC
                     ARIGATHTVVFGGFAAESLRDRINDSGAKLVLTQDGALRRGNVVELKATVDKAVQDAP
                     SVEKVVVYRRLGQERLPIEMKAGRDLDWNELLASARPNADRDGTPEIVEAEHPLFILY
                     TSGSTGKPKGVLHTTAGYLAGAHLTSKYVFDLRDEDIYFCTADVGWVTGHSYIVYGPL
                     SNGATCVLYEGAPNFPDWGRFWQIIEKHKVSILYTAPTAIRAFIRQGTEWPNKSDLSS
                     LRLLGSVGEPINPEAWVWYYENI"
BASE COUNT          543 a         1057 c         1003 g          538 t
ORIGIN      
        1 cctcgctgcc gtcggtcccg cagcggcagc gctcgtcacc tctcctgctc cgcccgaggc
       61 cgggtcggtg tcgccccccg catctgccgt ggctcccgcc gccgtgcccg cgacttcggc
      121 cgttcccgtg gcgaaatcgc gaaaccgtgc cgccccccgc gcctccgccg atcgagcgcc
      181 catccgtcgc ggtgttgctg gcgggctcga gctcagcaac cgagaacctt gacgcgcggt
      241 tccctagcgc tccccgactt ggtagatgag tggggagtat ggtggaaggc gtccgcccgg
      301 tccttttcgg cttcttgctc ggggtgctcg cgacagcgca cgcccgcgcg gacgagcctg
      361 agacggatgc cagcgcccag gccgcagacg ccgcaaccca gagcgctcgc cggcacttcc
      421 gggtcggggt gaagctttat cgggacgcga attatccggg agcgctcgcc gagttcgagg
      481 ctgcgtaccg cgacaagccg gggccgggct ctctgcagaa cgtcgctttg tcattgaagg
      541 cgctctttcg ctacgccgaa gcggcgagca ctctgcgcct gctgctcgag cgacacgcga
      601 ccgaactatc ggattcggaa cgcgccgcga tgcagaccgc gctcgccgag ctcgaaagct
      661 tggtcggcac gctggtgatc gcgattcagc ccgcagaagc gagcgtgacc atcgacgggc
      721 gcgcaaccac tcgcgacgac atctcacgag gcgtctcgct gaatgtcggc gagcacacgg
      781 tcgtggtcga agcgcgcggt tacgaacgcc gcgcgcaaat cgtgcgcatc gcgagcctgc
      841 aacggctcgc gctcaacgtg gacctgaaac ccacggctgg gttcattcac gtggtcgcga
      901 gcgatccgaa ggcggcaatc gcgatcgacg acgagccgct cgcttatcac acgtatcgcg
      961 gtcccctgac gcctgacgtc gaacacgtgg tccaagtcta ccgggcaggg ttcgagccgt
     1021 tcgagaagat ggtgcgggtc gagctcggga ggaccgtgga cgtgcaggcg gagctcggcg
     1081 ctcctaccgg cgtcgtcgac gaccgagccg aactaccgaa gaagccggtg gctccgccgc
     1141 cttcacgcaa accggtcggc tattacgcgc tcgcgacggt cgcggccgtg ggtctcaacg
     1201 acacgcccct caacctcagg gttgacggct cgagtggcat cacgctcccg tcgttcggat
     1261 tgcgcggtgg atatcgcctc tccgagccgg ttgcggtgga gctcgccgtc gacttcggcc
     1321 gtctcgaagc caaggacgct tgtgagctcg accaatcgga cacttgtgtc acgaagcgcg
     1381 atttttcgct ccgctcattt cgattcggcc ccaatcttcg actcatgaca cacggcgagg
     1441 tcgtgcgatt cgcgacgtcg gtcggggctg ggctcgtgag tcacacgctc tccctttccc
     1501 gcgatgaggc gagcgcccgc gccggcggct cggccagcgg cgtcgacccg tacttctctc
     1561 tcgagctcgg cgccgccttc aactaccggc accttttggc ggaggtgggc gctattgcat
     1621 tcatcgaagg ggctaccggc cttcacggcg ccttcgacga acagaccgaa cgcgccgtgt
     1681 tctcgagcgg caccttaccg atgctcgggt tcgttctcaa actcggttac agcgcgtggt
     1741 cgccccggaa ttgagccgat tctgcgccgt catgatccgc gtcgcgaggt gataaatcac
     1801 tcgccgcagg gaggctgggg cagtagcata tgcgagcatt ccgggccggt tcggaggctt
     1861 ctgagtcggc catttcacca ccaaaccagg gcagccatga gtcacgacac gacggcaatc
     1921 gagagccatt cgagtgagac ccgcacgttc gagccgagtc cggagtttgc acagctcgcg
     1981 cggattcgct ccgcggagga gtatcagaag ctctaccgcg agagcctgga ctcgcccgag
     2041 accttctgga agcgcgaaac gtcggagctc gtgtttcgga cgccgtggaa acagctgctc
     2101 gactggaagc tccctcacgc gaaatggttc ctgggagcca cgctgaacgt caccgagagc
     2161 tgcctcgaca agcacctttc cggcgcgcgt cgcgacaaga ccgcgatcat ttgggagagc
     2221 gagccgggcg agtcgaagaa gctcacgtac gccgagctgc atcgcgaagt ggtcgagctc
     2281 agcgctgccc tgcgcggcct cggcatcaag aagggcgatc gcgtcgccat ctacatggga
     2341 atggtccccg aggtggtcgt cggtatgctc gcgtgcgcgc gcatcggcgc aacgcatacg
     2401 gtcgtcttcg gagggttcgc cgccgagtcg ctacgcgatc gcatcaacga ttccggagcc
     2461 aagctcgtgc tgacgcaaga cggcgcgctc cgtcgcggca acgtggtcga gctcaaagcc
     2521 accgtcgaca aggccgtgca agacgcgccg agcgtcgaga aggtcgtcgt gtaccgtcgc
     2581 ttgggacaag agcggctgcc catcgaaatg aaggccgggc gcgatctcga ctggaacgaa
     2641 ctcctcgcat ccgctcgccc caacgccgat cgcgacggca ctcccgagat cgtcgaggcc
     2701 gagcacccgc tcttcatcct ctacacctcg ggctcgacgg gcaagccgaa gggcgtgctg
     2761 cacacgacgg ctgggtacct cgccggcgcg catctcacga gcaagtacgt cttcgacctg
     2821 cgcgacgagg acatctactt ctgcaccgcc gacgtcggtt gggtcaccgg gcacagctac
     2881 atcgtctacg gtccgctctc gaatggcgcc acgtgcgtgc tctacgaagg cgcgccgaac
     2941 ttcccggatt ggggccgctt ctggcagatc atcgagaagc acaaggtcag catcctgtac
     3001 acggcgccca ccgcgatccg cgccttcatt cgccaaggca cggagtggcc caacaagagc
     3061 gacctttcga gcttgcgctt gctcggcagc gtgggtgagc cgatcaaccc cgaagcctgg
     3121 gtttggtact acgagaacat c
//
LOCUS       DASTKS010000732        10057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1457329, whole genome shotgun sequence.
ACCESSION   DASTKS010000732 DASTKS010000000
VERSION     DASTKS010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457329"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1312
                     /locus_tag="VFQ35_15165"
     CDS             119..1312
                     /locus_tag="VFQ35_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9932041.1"
                     /translation="MSRALSFAIFFSVMLSLVGGAHYYLWARLVRDPGFSPGIFKALT
                     AALVALFVSIPLSLVLARTLTGPFARAAIWVAFVWLGMTFILLVVLGGLDLLRNAALL
                     ASHYLGHAEPIDPERRRLLSRILAGTVALIGGSLGLTALRSGLGRIRTHEVRVALERL
                     PSELDGTTIVQLSDVHIGPTLRREFLEEVVRQANALNPDVIAITGDLVDGSVADLREH
                     VAPLRELRARHGVFFVTGNHEYYSGVDEWCVELERVGIRVLRNERVSVGIGDISFDLA
                     GIDDFSSAQFGNGHGPNLPRALDGRDPSRELVLLAHQPRAVFEARQHDVGLQLSGHTH
                     GGQIWPWNFMVKLQQPVVSGLARFGRTWVYVSNGTGFWGPPMRLGAPAEITRVVLLAP
                     AEAVA"
     gene            complement(1319..2863)
                     /locus_tag="VFQ35_15170"
     CDS             complement(1319..2863)
                     /locus_tag="VFQ35_15170"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932042.1"
                     /translation="MPALRAAAARTLRISPEELPELVLRKRSIDARRGGVRFHLVLGV
                     REASAPPEIARRPRSLGESQRVLVVGDGPAGLFCAYELACHGVGALVLDRGKPVQPRR
                     HDLKGLNRRGVVDPDSNYCFGEGGAGTYSDGKLYTRSHKRGSVRDVLLALADNGAPEA
                     ILTDARPHIGSNRLPQVVTSLRTRLEAVGTEFVFGAKVTGLLLEGSGAARRVCGVRSA
                     DGREFLADAVVFATGHSARDVFQLLLDAGLPLEAKPFALGVRIEHPQPLINRIQYGRF
                     AEHPKLPNAAYRLVQQVDGRGVFSFCMCPGGWVVPASTEPDGLVVNGMSLSRRSSPHA
                     NSGLVVAVEPADWERAGFSGPLGGVELQRAIERAAAKAGGGALVAPAVRATDFARGRP
                     SSTVPESSYIPGIVAADVAAVLDAAGVPIARRLSQALEAFNRRMPGYVTEEAVLIGVE
                     SRTSAPVRVPRDDESLEARGLAGLYPAGEGAGYAGGIVSAALDGVRVAHAILKKRGIA
                     VEASGD"
     gene            3139..3657
                     /locus_tag="VFQ35_15175"
     CDS             3139..3657
                     /locus_tag="VFQ35_15175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932043.1"
                     /translation="MARGNSEALGALYDRYAPLLLALVERVAARGAEAEDLVHDVFLE
                     AWRRAADYDAARGTVKSWLLLRARSRALDHKKSARVARHGGGSDSVFLEQISDGSAEP
                     SLAPDQSRLREVLLSLPSEQRTVLVLGYFEGMSSSEIAERVGIPLGTVKSRVAAALAN
                     LRQTLADRRQGP"
     gene            3654..4217
                     /locus_tag="VFQ35_15180"
     CDS             3654..4217
                     /locus_tag="VFQ35_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9932044.1"
                     /translation="MKTPPEALDDALGLASKEGELLSTLAEALPARAPGHALRARLLA
                     SAASPRLRWAPFFDKLGALFDLDEAALMQMAERAALEDQWQPGPLPSVSLFHLSGGPA
                     IASADAGLVRVAAGFEFPTHRHLGEERTLILEGEVRESSGLLRRPGDALSMPADSSHS
                     FEVVSSKPLLYALVLFGGVEIGGIKFP"
     gene            complement(4281..6065)
                     /locus_tag="VFQ35_15185"
     CDS             complement(4281..6065)
                     /locus_tag="VFQ35_15185"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9932045.1"
                     /translation="MVRIVRARTAWCIVLVTSLFAGASGCILGNFEIEGALGGGGIAG
                     GGSAGASFAGMGSAGIGGSAGGVSRISPPTLVADRYVMLQGTTLVRDAAAGLLANDTP
                     VNLSVTGFTNVDPSRPRLFDADMTLERDGSFRFTPKPRFFGTYKLSYTAENQVGIRAT
                     SDIEIRVVPSEIDLDAIVVGIGGYVLYGAKSEALGAVLDGAGDVNGDEVSDLLIGAPN
                     ANSGDGAAYVVFGKTDLDSVDVEPVSLGSKEKRFLCVLGAPAEKLGSSVAGLGDVDGD
                     ELGDLAIGASGENGRVYVTQARDLSVVNRLPLAHGYSIAGDSANVAVGRLLEAGGDLD
                     GDGVPDALVSALNGSTGWLHLPFGDPELTGQLGVTGLPGAHVRGAVEGDAFPLDAAFV
                     GDLDGDGGDEVLAASNTAFYLFSGNGGFPVDAGAATVGGTRGNYRAARVAEGSASVTG
                     LGDVNGDSAKDFAYCDGTAACTVVFAPIATLAQGTPVYGFTRDAQRLSVRGGGDVDGD
                     GLSDILFSDDQSAYVVYGNRAGLLPTDVSKLGQNGFSIIPASGGTITAASIVGDVNGD
                     GIDDLAIADAAADDGNGRVYVVFGLSSR"
     gene            complement(6163..7107)
                     /locus_tag="VFQ35_15190"
     CDS             complement(6163..7107)
                     /locus_tag="VFQ35_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932046.1"
                     /translation="MLLSAHRGEAATPDAADGPSLAPARELWLEGQQAYQSGDYVRAA
                     DRFDRAYELERVPPLGLWVARSLMQAGRWVDALDRYREVADQSLPSDASSRDWAARRV
                     AKLERQQLLPRVPNVIVLLAGAPSSEVTVEVNGTFLPSEFLGIARAVDPGPVRVHGEH
                     GKQTADANIDIAEGETRTVQLSFAEPPPKREPARSVAPPLRPSSRGEVQRVVSFVAVG
                     VGGAALLTGGVFATLAAGDEGRLLRDCPMSRCPASYRSNVETYESKKTVATVALWSGA
                     ALATAGLVLYFTLPKSTPSSRALGAFWAGSHAGVWGRF"
     gene            complement(7252..7740)
                     /locus_tag="VFQ35_15195"
     CDS             complement(7252..7740)
                     /locus_tag="VFQ35_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932047.1"
                     /translation="MLALVTSALLVAPSVVAEPLAAPKRAVRSAGVDEPNVPNVTFTG
                     FRRLSDGRALLYVELSAKVPVSVLRQQGAVIYRLEGARVALKNNKNPLLTGAFATNLL
                     SARLVTPKPARKGKGRAKEPSTRYVDVVLNLRAGVTPTHVMSDRPGGAVLEITIPAAG
                     GK"
     gene            complement(7915..8805)
                     /locus_tag="VFQ35_15200"
     CDS             complement(7915..8805)
                     /locus_tag="VFQ35_15200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET9932048.1"
                     /translation="MSSLIGISQAVSELVRKVQGAIVSVGSRHAASASGIAWEKDLVV
                     TAAHAIERDDKVSVVSSEGNCLEAECIGTFLPADLALLRVKGGELPAWERTARAELEL
                     GELVLALGRSGQRLRAKVGLVSFLGAEWRLGPGVRVDRYIESDIAPCPAFSAGALVRA
                     DGTLIGVNSARFGRGALVTLPTATVERVVAALSERGRVRRAELGVAVHPVQLPSTLGQ
                     RLGQSAGLIVMAVRPDSPAERSGLMLGDVLLRLGDAVLGGVEELENALGEGSIGQSLP
                     LLLVRAGERLELRTEPREKA"
     gene            9056..9670
                     /locus_tag="VFQ35_15205"
     CDS             9056..9670
                     /locus_tag="VFQ35_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9932049.1"
                     /translation="MLALTLAAAIVAESALVRSGLSSILNGSSQIRVVAEATPSEAKE
                     LPTEGVDVVVCDVADATSATSALENAPKGVPVLALVSAPEKARELVRAGVRGVLNRDA
                     PGERLRAASIAVAAGLCTLDEQTLEDLVSPRTAETRATVLTPREQQVLELLAEGLSNK
                     VIALRLDISEHTAKFHVNSILDKLEADTRTDAVMRAARSGMLAI"
     gene            complement(9725..>10057)
                     /locus_tag="VFQ35_15210"
     CDS             complement(9725..>10057)
                     /locus_tag="VFQ35_15210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9932050.1"
                     /translation="CIRDYIHVEDLVEAHIAVMEALRPSDLRVYNLGIGRGYSVREIL
                     DAARSVIIRPFTVETGPRRPGDPPVLYADASKISREIGWKAKRTDIATTIESAWKWFQ
                     AHPSGYLD"
BASE COUNT         1675 a         3496 c         3276 g         1610 t
ORIGIN      
        1 gccgctcccc gctccgcgac aattccggac gtgaccgcga cgattttggc ggagattgtg
       61 cgtctgttcg gtcgaagggc ttgccggaac gtcagcgcgg cgcatatgag cgggtgccgt
      121 gtcgagggct ttgtcgttcg cgatcttctt cagcgtgatg ctcagcctcg taggtggggc
      181 gcactactac ctctgggccc gattggtacg agacccaggt ttttcacctg gaattttcaa
      241 ggcgctgacc gcggctctgg tcgcgctttt cgtcagcatc cctttgtctc tcgtactggc
      301 ccgtaccctg accggccctt tcgcgcgggc tgcaatctgg gtcgcgttcg tctggctcgg
      361 catgactttc atcttgctcg tcgtgctggg cggcctggac ctcttgcgca acgcagcact
      421 gctcgcgagc cactatctcg ggcacgctga gcccatcgat cccgagcgcc gccgtctgct
      481 ttcgcgcatt ctggcgggca cggtggcgct cattgggggc agccttggcc tcaccgcgct
      541 tcgctcgggg ctcgggcgga tccgcactca cgaagtccgc gtcgcgctcg aacggctacc
      601 gagtgagctc gacggcacga cgatcgttca gctcagcgac gtacacatcg ggcccacgct
      661 gcgcagagaa ttcttggaag aggtcgttcg ccaagccaac gcgttgaacc ccgacgtcat
      721 tgcgatcacc ggcgatctcg tcgacggctc ggtcgcagat cttcgcgagc acgtcgctcc
      781 cctgcgtgaa ctccgagcgc gccacggcgt cttcttcgtc accggaaatc acgagtacta
      841 ctccggcgtc gacgaatggt gcgttgagct cgagcgcgtc ggcattcgcg tccttcgcaa
      901 cgagagagtc tcggtaggca tcggcgacat cagcttcgat ctcgccggaa tcgacgactt
      961 cagctcggcg cagttcggca acggccacgg ccccaacttg ccccgcgcgc tcgacggtcg
     1021 cgacccgagc cgcgagctcg tgctcctcgc gcatcagccg cgcgcggtgt tcgaggcgcg
     1081 ccagcacgac gtcggcctgc agctctcggg tcacacgcac ggcggccaga tttggccttg
     1141 gaacttcatg gtcaagctgc agcagccggt ggtctccggg ctcgcccgct ttgggcggac
     1201 ttgggtctac gtgagcaacg gtaccgggtt ctggggaccg ccgatgcgac tcggcgctcc
     1261 cgccgagatc acccgggtcg tcttgctcgc gccggccgaa gccgtcgcct agcgaacgct
     1321 agtcgccgct cgcttcgacg gcgatcccgc gctttttcag gatggcgtgc gcgacccgaa
     1381 cgccatcgag agccgcgctc acgatgcctc ccgcgtaccc ggcgccctca cccgcgggat
     1441 agagcccggc aaggccgcgg gcctcgagcg actcgtcgtc gcgtgggacg cgtacgggcg
     1501 cgctcgtccg cgactccacc ccgatgagca cggcttcttc cgtgacgtag ccgggcatcc
     1561 gccgattgaa cgcctccaag gcttgcgaga gccgcctcgc gatgggaacg ccggccgcgt
     1621 cgagcactgc cgcaacgtcg gcagccacga tgcccggaat gtagctcgac tccggcaccg
     1681 tgctcgacgg tcgtccccgc gcgaagtcgg ttgcacgcac cgccggcgcg accaacgcac
     1741 cgccgcccgc cttcgctgcg gcgcgctcga tcgcgcgctg gagctcgacg ccgccgagcg
     1801 gacccgaaaa gcccgcgcgc tcccagtccg ccggctcgac tgcgacgacg agccccgagt
     1861 ttgcgtgcgg cgagctgcgg cgcgaaaggc tcatgccgtt cacgacgaga ccgtccggct
     1921 cggtcgacgc cggaacgacc cagccacccg ggcacatgca gaacgagaac acgccgcgcc
     1981 cgtcgacttg ctgcacgagc cgataggcgg cgttcggcaa cttcgggtgc tcggcgaatc
     2041 ggccgtactg gatgcgattg atgagcggct gcgggtgctc gatgcgaacc ccgagcgcaa
     2101 acggcttggc ctcgagcgga agtcccgcgt cgagtagcag ctgaaacacg tcgcgagccg
     2161 agtgccccgt cgcgaacacc accgcgtctg ccaaaaactc gcggccgtcg gcggagcgca
     2221 cgccgcagac acgccgcgcc gcgccgctgc cttcgagcaa tagccccgtc accttcgcgc
     2281 cgaagacgaa ctcggttcca accgcttcga ggcgcgtgcg gagcgaagtg accacctgcg
     2341 gcaggcggtt actgccgatg tggggtcgcg catcggtgag gatcgcttcg ggcgcgccgt
     2401 tgtcggcgag cgccaagagc acgtcgcgta cgctgccacg cttgtgcgaa cgcgtgtaga
     2461 gcttgccgtc gctgtaggtt ccggcgccgc cctcaccgaa acagtagttg ctgtcgggat
     2521 ccacgacgcc gcggcggttc aaccccttga ggtcgtggcg gcgcggctgg acaggcttgc
     2581 cgcgatcgag aacgagcgcg cccacgccat ggcacgcgag ctcgtaagcg cagaaaaggc
     2641 ccgcggggcc gtcaccgaca acgagtacgc gctgcgactc gccgagggac cgcgggcgcc
     2701 gcgcgatttc gggcggagcg gaggcttcgc gcaccccgag gacgaggtga aagcgcactc
     2761 cgcctcggcg agcgtcgatg gagcgctttc ggagcacgag ctcgggcaat tcctcgggag
     2821 agattcgcag agtccgagcg gccgcggccc gcagcgccgg catatcgtca gcctgatcta
     2881 ggccgagttc gagggtgagc tcgtcggatc cctgcagagc gcgccgggta gaagcggggg
     2941 cgcgagaagg ccgggaatga ggatgcctag ggcgaggagg catggcgagt tggctccgcg
     3001 atatcacacc ggcgccgggg cgcccgtctc ggcgcttttc ccaggggcgc aacgacgcta
     3061 tcgtgggttt ccgtggcctc gaccgaagag tcttccgctg aagcggccga accgagcgat
     3121 gcagctttgg tggctgcaat ggctcgaggc aactcggaag cgctgggagc gctctacgat
     3181 cgctacgcgc cgctgctgtt ggcgctcgtc gaacgggtcg cggcacgagg agccgaggcc
     3241 gaagacctgg tgcacgacgt ctttctcgag gcgtggcggc gcgccgcgga ctacgacgcg
     3301 gcccgcggca ccgtcaaatc atggctcctg ctgcgcgccc gcagtcgagc actcgatcac
     3361 aaaaagagcg cccgcgtggc gcgacacggc ggcggcagcg acagcgtctt cctcgagcag
     3421 atcagcgacg gcagcgccga gccctcgctc gcgccggatc aatcacgcct acgcgaggtg
     3481 ctgctctcgc taccgagcga gcaacgaacc gtgctcgtgc tcggctactt cgaagggatg
     3541 agctcgagcg aaatcgccga gcgcgtcggg atcccgctcg ggacggtcaa gagccgggtc
     3601 gccgcggcgc tagcaaacct tcggcaaacg ctcgctgaca ggagacaagg accatgaaga
     3661 cgccgcctga agcgttggac gatgcgctcg gtctcgcgtc gaaagagggc gagctcttga
     3721 gcacgctcgc cgaagcattg ccggctcgcg ccccgggcca cgcgctgagg gcgcggcttt
     3781 tggcgagcgc tgcgtcccca cgcctgcgtt gggcgccgtt cttcgacaag ctcggcgctc
     3841 tcttcgatct cgacgaggcc gccctcatgc aaatggccga gagagccgcc ctcgaagacc
     3901 aatggcagcc cggccccctt ccgagcgtgt cgctctttca tctgagcggt ggccctgcca
     3961 tcgcgagcgc ggacgcgggc ctcgtccggg tcgcggcagg gttcgaattt ccgacccacc
     4021 gccatttggg agaagagcga actctcatcc tcgagggaga agtgcgcgaa tcgagcggcc
     4081 tcttgcgaag gcctggcgac gcgctcagca tgcccgccga ctcgtcgcat tcgttcgagg
     4141 tggtgtcgag caaaccgttg ctctacgcgc tcgtactttt cggcggagtc gagatcggcg
     4201 gcataaagtt cccttgagct cgagctcggc cacgcgggga accgaagttt ctgcccggtg
     4261 cgcgaggcaa gaaacgcggg ctaacgcgac gagaggccga acacgacgta gacgcgcccg
     4321 ttgccgtcat cggccgcagc atcggcgatt gcgagatcgt cgatgccgtc tccgttgacg
     4381 tcgccgacga tgctggcggc ggtgatggta ccgccgctcg cggggatgat gctgaacccg
     4441 ttttgaccga gcttgctcac gtcggtgggc aaaagcccag cgcggttgcc gtagacgacg
     4501 taggcgctct ggtcgtcgga aaagagaata tcggagagcc cgtccccgtc gacgtcgccg
     4561 ccgccgcgaa ccgagaggcg ctgcgcgtcc ctcgtgaaac cgtagacggg ggttccttga
     4621 gcgagcgtcg caatcggcgc gaacacgacc gtgcaagccg cggttccatc gcagtacgcg
     4681 aaatccttgg cgctgtcgcc gttcacgtcg ccgagccccg ttacggatgc cgacccttct
     4741 gcgacgcgcg cagcgcggta gtttccgcgc gtgcctccca ccgtcgccgc gcccgcgtcg
     4801 accgggaacc caccattgcc gctaaaaagg tagaacgccg tgttcgaagc cgcgagcacc
     4861 tcgtccccac cgtccccgtc gagatcgcct acgaacgcgg cgtcgagcgg gaaggcgtcg
     4921 ccctccacgg cgcctcgaac gtgcgcgccg ggcaacccgg tcacgccgag ctgacccgtg
     4981 agctccggat ctccgaaagg taaatgcagc cagccggtgc tgccattcag ggcggaaacc
     5041 agcgcgtcgg ggacgccgtc gccatcgaga tctccgcccg cctcgagcag ccgcccgacc
     5101 gcgacattcg cgctgtcccc cgcgatcgag tacccatgag cgagcggcaa ccggttgacg
     5161 acgctgagat cgcgggcttg cgtgacgtac acgcgcccgt tctccccgct cgcgccgatc
     5221 gcgagatctc cgagctcgtc cccgtcgacg tcgccgagtc ctgcgacgga gctgccgagc
     5281 ttttcagcgg gagccccgag cacgcacagg aagcgcttct ctttgctgcc gagagacacg
     5341 ggctcgacgt cgaccgagtc gagatcggtt ttaccgaaca cgacgtaggc cgcgccgtct
     5401 ccgctgttcg cgttgggagc gccgatgagt aggtcggaga cctcgtcgcc gttgacgtcg
     5461 cctgcgccgt cgagcaccgc ccccaaggct tctgattttg ctccgtagag cacgtatccg
     5521 ccgatgccca ccacgatcgc gtcgagatcg atttcgctcg gtaccacgcg aatctcgatg
     5581 tccgaagtgg cgcgaatgcc gacctggttc tccgcggtgt agctcagctt gtaagttccg
     5641 aaaaagcgcg gtttcggcgt gaatcgaaac gacccatcgc gttcgagcgt catgtccgcg
     5701 tcgaacaggc gcgggcggct cgggtcgacg ttcgtgaagc cggtgacgct gaggttcacg
     5761 ggggtatcgt tcgcgagcag ccccgccgcg gcatcacgca cgagggtcgt gccttgcagc
     5821 atgacgtacc ggtccgcgac cagcgtcggc ggtgagatcc ggcttacgcc gccggcgctg
     5881 ccgccgattc cggccgaccc cattcccgcg aacgacgctc ccgcggagcc tcctcctgcg
     5941 atccctcccc cgcccaacgc gccttctatt tcgaagttac cgaggatgca ccccgacgcc
     6001 cccgcgaaca ggctcgtcac cagcacgatg caccaagcgg tgcgcgctct cacgatcctg
     6061 accattcatc acatctcgca ggcaggtaag ccgtcgtcgc cttcgctcct cgccacttcg
     6121 gcagggaagg cttcattccc aggagacgca gctccttgcg gctcaaaatc tcccccagac
     6181 tccggcgtgc gaccccgccc aaaacgcccc gagcgcgcgc gagctcggcg tgctcttcgg
     6241 cagggtgaag tagaggacga ggccagccgt cgcgagcgcc gcgccggacc acagcgcaac
     6301 ggtcgcgacc gtcttcttgg actcgtaagt ctcgacgttc gagcgataac tcgcggggca
     6361 gcgcgacatg gggcagtcac gcaggagccg cccttcgtca cccgcggcca gcgtggcgaa
     6421 cacccccccc gtgagcaacg cagcgccgcc gacgccgacc gcgacaaacg agacgactcg
     6481 ctgaacctcg ccgcggctcg atggccgcaa tggcggcgcg acgctgcgcg ccggctcgcg
     6541 cttcggaggg ggctcggcga acgagagctg cacggtccgc gtttcgcctt cggcgatgtc
     6601 gatgttcgcg tcggcggttt gcttgccgtg ttctccgtgc acacgcacgg gacccggatc
     6661 gacggcgcgg gcaattccga ggaactcgct cggcaagaaa gtaccgttca cctcgaccgt
     6721 gacctccgag ctaggcgctc ccgccagcaa aacgatgacg ttcgggacgc gcggcaagag
     6781 ctgttgccgc tcgagcttcg ccacgcgacg cgcggcccaa tctcgcgacg aggcgtccga
     6841 ggggagcgac tggtcggcga cctcacggta tcggtcgagc gcatcgaccc agcgccccgc
     6901 ctgcatcaga ctgcgcgcga cccaaagccc gagcggtggc acgcgctcga gctcgtaagc
     6961 ccgatcgaaa cggtcggcgg cgcgcacata gtcgccgctc tggtaggctt gctgaccctc
     7021 gagccaaagc tcccgtgccg gcgccaggct cgggccatcg gcggcgtcgg gggtcgcggc
     7081 ctcgcctcga tgagccgaga gcaacacgag cgcgccgaac gccgcgcacc gcgcccaccg
     7141 accaaaactc gaacgccgag cagtcgaact gctcgacggc ctcggaagga acggcgaacg
     7201 gcgaatgggc actgcctagc tttttagcag gcaatgccgc ccgttgccgc gctacttacc
     7261 gcccgcggcc ggaatcgtta tctcgagcac ggctccccct gggcgatcgc tcatgacatg
     7321 ggtgggggtt acccccgcgc gcaggttcag gacgacgtcc acgtagcgcg tgctgggctc
     7381 cttggcgcgt cccttccctt tgcgcgcggg cttgggggtg acgagccgag cgctcagcag
     7441 gttcgtagcg aacgctcccg tgagcaacgg attcttgttg tttttgagcg cgacgcgcgc
     7501 gccctccagt cggtaaatca ctgcgccctg ctgccgcagt acgctcaccg gcaccttggc
     7561 gctgagctcg acgtaaagca gtgcgcggcc gtcggacaag cggcgaaacc ccgtgaacgt
     7621 cacgttcgga acgttgggct cgtccacgcc ggcggagcgc actgcgcgct ttggagccgc
     7681 caacggctct gcaaccaccg acggcgccac caacaacgcc gaagtaacga gggcgagcaa
     7741 tcgggcgcgc cgcggccgtg caggaggtgc gggcggttcc gaagagcgag gaggcatgcg
     7801 accccccaat tgtgggccca gtgggcgtca gtggaaaaga ttcgcgtacg cgaaaacgtc
     7861 ggcccaaagg ccgctcccgg tagacgcggc tcgccgggat tccactctgc gccctcaggc
     7921 cttttcgcgc ggctcggttc ggagctcgag gcgctcaccg gcgcggacga gcaggagcgg
     7981 cagcgattgg ccgatcgagc cctcgccgag agcattctcg agctcttcga ctccaccgag
     8041 caccgcgtcg ccgagcctga gcaacacgtc gccgagcatc aaaccggagc gctccgcggg
     8101 gctgtccggg cgcaccgcca tgacgatgag ccccgcgctc tgtccgagcc tctgaccgag
     8161 agtgctcggc agctgcaccg ggtgcaccgc gacgccgagc tccgcgcggc gcacgcgtcc
     8221 gcgctccgag agtgccgcga cgacgcgctc gacagtcgcg gtgggaagag tgactaaggc
     8281 gcccctgccg aagcgcgcgc tgttcacgcc gatcagagtc ccgtcggcgc gcaccaaagc
     8341 tccggccgaa aaagcggggc acggtgcgat gtcactctcg atgtagcgat ccactcgcac
     8401 ccccggaccg agccgccact ctgcccccaa gaagctcacg agaccaacct tcgcgcgcaa
     8461 acgttgaccg ctgcgcccga gcgccagcac gagttcgccg agctcgagct cggcgcgcgc
     8521 ggtccgctcc cacgccggaa gctctccgcc ctttacccgg aggagcgcga gatcagccgg
     8581 caagaaggtg ccgatgcact ccgcttcgag gcagttcccc tcgctcgaaa cgacgctgac
     8641 cttgtcgtcg cgctcgatgg cgtgcgccgc cgtgaccacc aaatccttct cccaagcgat
     8701 tccgctcgcg ctcgctgcgt gtcgcgaacc cacgctgacg atggctccct gaacctttcg
     8761 aacgagctca ctcactgctt gcgaaatgcc gatcaggctg gacatggctt ctccttgcga
     8821 cgaccggctg agccgtcgtc tcggaaccct acgacccgcc gcatgggagc gaaactctcg
     8881 gatcggcgag tcgaacctgc ccgatcagac gggacgatgc cgtcaagcag cgagctttgg
     8941 ctcacaccgg gcgccgaatc gccgaatcgc cgaaacaccc gtgacactcg tgaaccgaga
     9001 ggaaaaagcg gagcgcccct ttacagccct tctccgcgac ttatggtgcc ctactatgct
     9061 ggctctcacc ctggcggcgg cgatcgtggc cgaatcggcg ctcgtccgca gcgggttgtc
     9121 ttcgattttg aacggttcat cgcagattcg cgtcgtcgcg gaagcgaccc cgagcgaagc
     9181 gaaagagctc ccgaccgagg gagtggatgt agtggtgtgt gacgtggcgg acgccacctc
     9241 ggcgacgagc gccctcgaga acgcgcccaa gggagtcccc gtgctcgccc tggtcagcgc
     9301 gcccgaaaag gcgcgcgagt tggtccgcgc tggagtgcgc ggtgtcctga accgcgacgc
     9361 tccaggagag cgactccgtg ccgcctcgat cgcggtcgcg gcaggtctct gcactctcga
     9421 cgaacaaacc ttggaggacc tcgtttcacc gcgtactgct gaaacgcgcg cgaccgtgct
     9481 caccccacgc gagcagcagg ttctcgagct tctcgcggag gggctctcga acaaggtgat
     9541 cgcgctgcgc ctcgacatca gcgaacacac ggcgaagttt cacgtcaact cgatcttgga
     9601 caagctcgag gccgacaccc gcaccgacgc ggtgatgcgc gccgcgcgct ccggaatgct
     9661 cgccatctga gctatcgggc tcgaggttgg cggtgtcacc gaacgccgcc gctccgaccg
     9721 caattcagtc gaggtaaccg ctcgggtgcg cttggaacca tttccatgcg ctctcgatgg
     9781 tagttgcaat gtcggtccgc ttggctttcc agccgatctc ccgagagatc ttgctcgcgt
     9841 cggcgtagag caccggtgga tctcctggac gccgggggcc cgtttcgacg gtgaacggac
     9901 ggatgatcac gctccgcgcc gcgtccaaga tctcgcgcac gctgtacccg cgaccgatgc
     9961 ccaggttgta gacgcggaga tccgaagggc gcagagcttc catcaccgcg atgtgcgctt
    10021 cgaccagatc ctcgacgtgt atgtagtcac gaatgca
//
LOCUS       DASTKS010000733         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1009290, whole genome shotgun sequence.
ACCESSION   DASTKS010000733 DASTKS010000000
VERSION     DASTKS010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009290"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..630)
                     /locus_tag="VFQ35_15215"
     CDS             complement(70..630)
                     /locus_tag="VFQ35_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932051.1"
                     /translation="MQKRGATFGVALCGLVVGCGSADGDPQPDDRAKQFEFNELVRPA
                     PCFVRELPVAGDGAACVLTKTKLLGQTSCDCEKVGLGVAPPELASATKQTLRRESLCD
                     APGGLACESLCVCVVPQLHDELGERCRNDPNAEDIAGWCYVAPAQGLGNASQLETCSA
                     DNARELRLFGVTPSENTILTLSCYAP"
     gene            complement(732..1436)
                     /locus_tag="VFQ35_15220"
     CDS             complement(732..1436)
                     /locus_tag="VFQ35_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932052.1"
                     /translation="MSLIASEPLAVTSVVSLVKGEESVPLQPQVGGAPPSIATFQTGN
                     FLPFGSTWQVQATGEDLAGLPITTAEPVVVSTDPGVFAEDGFEAKPSMAVEGGVALVS
                     QVGGVPALSGSQSLLMHSDAVVLFHLSRRAGATAVRFTAQMFSMNADSVSLPSIVAGV
                     IGESKYLQVRSTPSDSPVVPTMDPSLAYAGPKQEVLLPIGEDGSDIALKIQMRMKCST
                     FCTDSRALLIDDLRVE"
     gene            1851..2159
                     /locus_tag="VFQ35_15225"
     CDS             1851..2159
                     /locus_tag="VFQ35_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932053.1"
                     /translation="MSFGARRIVNGNSVQRTKGALNDIVMFTSHELSPSLVGTSCGSP
                     PLPLEPAAPKPPTPPPALPPCVAPPKVDYPATLPPPPSSVIDPPLLPPCRPTAPPIAS
                     "
     gene            2294..>3686
                     /locus_tag="VFQ35_15230"
     CDS             2294..>3686
                     /locus_tag="VFQ35_15230"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HET9932054.1"
                     /translation="MASELGADDALADANDRFHAAYERSLDLQPNGPVIVLLNGELVI
                     RNGAVRRAFPLESGEFTAAKIAAHLTVATFLLANDEERLRPTVQRVDSMLEACSRGEP
                     TRLRTAMRTLLEASQRFGSRALQDGPLGERARSEFAASTREGLLRLTELATEAELIAL
                     HDATRAALSSLSERERETLEIVVAGNHQARSRSLGMQYFARRLGEEEGEERRVAYGEN
                     IETEDEALELVATRRLDRELAAAFFGDERRMQRDLLADAATRCLDRMNLDEPGTGRPS
                     DSPRNDPNANDTKRELGFLRRNGLSLMAGALFVASLSGQLATGFFAHNAELEDHGAAS
                     LSFFQYLASGHCIEAIFENWESEFLQMALFVVLTVMLRQQGSSESKNLDAPEAVDEEP
                     RKHRDEADAPWPVRHGGLALGMYRHSLSTAFFALFVSSFALHAFGGLRAANAERALHG
                     LSAQTAWEFVSSAE"
BASE COUNT          680 a         1236 c         1134 g          636 t
ORIGIN      
        1 ccctccgccc atcaagttag ctaatcgtgc cgtccgcggc cagcccattc tcgtggtgtc
       61 tcgttccctt tacggcgcgt agcagctcaa ggtcaggatg gtgttttcgc tcggtgtgac
      121 cccaaacaag cgcaactcgc gggcgttgtc ggcgctgcaa gtctcgagtt gggaagcgtt
      181 tccaagaccc tgcgccggag cgacgtaaca ccagcccgcg atgtcttcgg cgtttgggtc
      241 gttgcggcag cgctcgccca gctcatcgtg caattgcggc accacgcaaa cgcaaagtga
      301 ctcgcacgcg agtccgccag gcgcgtcgca aagagactcc cttctcagcg tttgcttcgt
      361 cgcggaagcg agctcggggg gcgccacacc gagcccgacc ttttcgcaat cacaggatgt
      421 ctgaccgagg agcttcgtct tcgtcaaaac gcaggccgcg ccgtcgccag cgaccggcag
      481 ctcgcgcaca aagcaaggag caggccgtac gagctcgttg aactcgaact gcttggcgcg
      541 gtcgtcgggt tgagggtcac cgtccgcgct tccgcagccg actactaggc cgcacagcgc
      601 aactccgaag gtcgctcctc gcttctgcat gcggcgtagg tgcgacgttc gagcgatttc
      661 ggctcacgcg aattcgtatt tttctcgaac ctgctctatc acatcgagaa ccagcctacg
      721 gcgcccacag gctactcgac gcgtaggtca tcgatgagta gcgcgcgact atcggtacag
      781 aacgtgctgc acttcatcct catttgaatc ttgagcgcta tgtccgagcc gtcctcgccg
      841 attggaagta agacctcttg cttggggccc gcgtaggcta gcgacggatc catcgtgggg
      901 actacgggcg agtcgctcgg ggtgcttcgc acttgcaaat acttcgattc gccgatcaca
      961 cccgctacga tggaaggaag gctaacgctg tcggcgttca tcgagaacat ttgcgccgtg
     1021 aagcgaacgg ccgtagcgcc cgcgcgccgc gacaggtgaa agaggacgac cgcatccgaa
     1081 tgcatcagga gcgattgcga accgctcaac gcgggcacac cgccaacctg cgaaacgagc
     1141 gcgacgcccc cttcgacggc catgctgggc tttgcttcga agccgtcctc tgcaaacacg
     1201 ccggggtccg tcgacaccac caccggctcc gcagtggtga tcggcaggcc cgcgagatct
     1261 tcgcccgtcg cttgaacttg ccacgtcgac ccaaagggta agaagttccc cgtttggaag
     1321 gttgctatcg acggcggggc gcctcccacc tgcggctgca gaggcaccga ctcctccccc
     1381 ttcacgagcg aaacgacgct ggtcacggcg agcggctcgg aagcgatcaa cgacacgccc
     1441 gagagcgggt cgaccggcgc ggcaacgacg agcgatggcg gggtccgatc gggcgagccg
     1501 cgcagcgcaa attcgaacct gcgaaacgag cgcgacgccc ccttcgacgg ccatgctggg
     1561 gctttgcttc gaagccgtcc tctgcaaaca cgccggggtc cccgatgacc gcgtaaccca
     1621 acgcggtgcc gcgcccgtcg agacgatccg cgacaccgtc gccattcgaa tcgctacccc
     1681 agatgacgag ctcggaaatt tcgaggcttc ggaaggagca attgggtacg tttttgatct
     1741 cgatctcgaa cgttgcggag agcgcgtagg caccctgcgt aacgtcgttg acggcgctca
     1801 aagctgggtt cgcgactgcg ctgccgcgac cgaggaccga actgagccca gtgtcgttcg
     1861 gcgcgaggcg catcgtgaac ggcaactccg tgcaacgcac gaagggggcg ctgaacgaca
     1921 tcgtcatgtt cacgtcccac gagctttcgc cgagcttggt tggaacgagc tgcggctcgc
     1981 caccgctacc gctagagcct gcagcgccaa aaccgccaac tcccccgccc gcgctccctc
     2041 cctgcgtcgc tccgccaaag gtcgactacc ccgccacgct gccgcctccg ccaagctcgg
     2101 tgatcgatcc gccgctgctg ccgccttgca ggccgacagc accccccatc gcgtcctgat
     2161 tacccaggcc cgcggagccg ccgcaagcgg tcaggaaagc gaaccggcaa gcatcgcata
     2221 cctcccctcc caagagcgac gtggagcgtg cgcaggcagg tcgcgctggc tcggcatttg
     2281 cttacccagg ggcatggcat cggagcttgg cgccgatgat gcgcttgcgg acgcaaacga
     2341 tcgcttccac gccgcgtacg agcgcagcct cgatttgcag ccgaacgggc ctgtaatcgt
     2401 gctgctgaac ggcgagctcg taatccgcaa cggagccgtg cggcgagcat tccctctcga
     2461 gagcggcgaa tttaccgccg cgaagatcgc ggcacatctg acggttgcga cgttcttgct
     2521 cgccaacgac gaggagcgac tgagaccgac cgtccagcgc gttgattcga tgctcgaagc
     2581 ttgctcccgc ggcgaaccca cccggctccg caccgcgatg agaaccttgc tcgaagcgag
     2641 tcaacgcttt ggctctcgcg cgttgcaaga cggcccactc ggtgaacgtg cgcgctcgga
     2701 gtttgcggct tcgacgcgcg aaggactctt gcgactcacc gagctagcaa cggaggctga
     2761 gctcatcgcg ttgcacgacg cgacgcgggc cgcgctcagc tcgctcagcg aacgtgaacg
     2821 cgaaacgctc gaaatcgtgg tggcgggcaa ccaccaagcc cgctcacgca gcctcgggat
     2881 gcagtacttc gctcggcgct tgggcgagga ggaaggcgaa gagcggcgcg tcgcctacgg
     2941 agagaacatc gaaacggagg acgaggcgct cgaactcgtc gccacccgtc gcctcgaccg
     3001 agagctggca gctgcatttt tcggcgatga gcggcgcatg cagcgcgacc tcctcgccga
     3061 cgctgcaacg cgttgcctcg accgcatgaa cctcgacgag cccggaaccg ggcggccttc
     3121 ggactcaccc cgcaacgacc cgaacgcgaa cgacacgaag cgcgagctcg gattccttcg
     3181 ccgcaacggt ctcagcctca tggcgggagc gcttttcgtg gcttcgcttt cggggcagct
     3241 cgcgaccggc tttttcgcgc acaacgccga gctcgaggac cacggcgcgg catcgctgag
     3301 cttcttccaa tacttggcga gcggtcactg catcgaggcg atcttcgaga actgggagag
     3361 cgagttccta caaatggccc tgttcgtggt gctcacggtg atgctccggc aacagggctc
     3421 ctccgaatcg aagaacctcg acgcgccgga ggccgtggac gaagagccgc gtaaacatcg
     3481 cgacgaggcc gacgcgcctt ggcccgtgcg acatggcggt ctcgctctgg gtatgtaccg
     3541 tcactcactt tcaacggcct tctttgcgct gttcgtctcg agtttcgcgt tgcatgcttt
     3601 cggtggcctg cgcgccgcga atgccgagcg cgccctgcac ggactttctg cgcaaacggc
     3661 gtgggagttc gtgagctccg ccgagt
//
LOCUS       DASTKS010000734         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_786224, whole genome shotgun sequence.
ACCESSION   DASTKS010000734 DASTKS010000000
VERSION     DASTKS010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786224"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..649)
                     /locus_tag="VFQ35_15235"
     CDS             complement(122..649)
                     /locus_tag="VFQ35_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932055.1"
                     /translation="MVSSAKPGSAFELLFLFAALACGIALFATLPLVAGRTSLRCERS
                     GPAVSCAIQTDYFGWFGETERIALRSVSTETHRTRLRNSDFYSKILVLNSTRELSGSK
                     LDPDTAESTLRELLAGGITAAPPMVVQEPSHGLTFGLLLGGMMFTGLSLVGLKHANWG
                     VAPGLRSGHRGRKRP"
     gene            complement(680..1030)
                     /locus_tag="VFQ35_15240"
     CDS             complement(680..1030)
                     /locus_tag="VFQ35_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932056.1"
                     /translation="MTKSAARRFGLQEVLALIGGALVVLHVLLSRLSDPTSAFWAWLL
                     TAGLCVFWALVILLATLVLLVRRNHSPRWWLPLTAIGIFAALGVELAVKGLQALAWLE
                     QLLRSIGDPNSGRF"
     gene            1435..1818
                     /locus_tag="VFQ35_15245"
     CDS             1435..1818
                     /locus_tag="VFQ35_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932057.1"
                     /translation="MVIDWTAGIGGMGAGGATSASGGMVSVGGAVASSNTVEIDWADV
                     LPSTEAACMDSFLAAYHFADNGSGSWVAVDFASANGTWRAPWCVGPSVVFSDIAPRAR
                     IAVTARSKQASTAPTRAFFIGFSPP"
     gene            complement(1855..>2056)
                     /locus_tag="VFQ35_15250"
     CDS             complement(1855..>2056)
                     /locus_tag="VFQ35_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932058.1"
                     /translation="APYAAAYVAPDGDRRPFNYVVMEPPQSGLRFTVKCLSSEARCTG
                     GVLDRFEVAYPALPTSAQSRCS"
BASE COUNT          394 a          622 c          681 g          359 t
ORIGIN      
        1 ttccgccgac accgccgagg ctgagccgcc cgaaagcgcg caacagtcat cgcgacgcag
       61 cgaggcgtga aagtcaccag cgtgagcggg tgagacgata aggtcgaagc gccgctcgct
      121 ctcagggtcg cttgcgtccc cggtgccccg acctcaaacc gggcgcgacc ccccagttcg
      181 cgtgcttgag cccgacgaga gagagcccgg tgaacatcat tcctccgagc agcaggccaa
      241 acgtaagacc gtgggatggc tcctggacga ccatgggtgg cgccgcggtg attccgcccg
      301 cgagcaattc ccgaagagtg ctttcggctg tgtcgggatc gagcttcgag ccgctcaact
      361 ccctcgtcga gttgaggacg aggatcttgg aatagaagtc actattgcga agtcgcgtgc
      421 ggtgcgtctc cgtcgagacg gaccgtagcg cgatgcgctc ggtctcgccg aaccaaccga
      481 agtagtcggt ttgtatcgcg caagatacgg cgggcccgct tcgctcgcag cgcagcgacg
      541 tgcgaccggc cacgagcggg agcgtcgcga acagcgctat tccgcacgcg agcgcggcga
      601 agaggaacag aagctcgaaa gcgctgccag gttttgctga ggaaaccacc gtcgacccgc
      661 aaggttgcga tcgttcgctt caaaagcgcc cgctgttcgg atccccaatg cttcgtagga
      721 gttgctcgag ccatgccagc gcctgaagtc ccttgaccgc aagctccaca ccgagcgcgg
      781 cgaagatgcc gatggctgtc agcggtagcc accagcgcgg cgagtggttt cgccgaacga
      841 ggagcacgag ggtcgcgagc aggatgacca gcgcccagaa gacgcagagc cccgccgtca
      901 acagccacgc ccaaaacgcg gacgtcggat cgctgagccg cgagagaagc acgtgcagga
      961 cgaccaaggc cccgccaatc aacgcgagta cttcttgcag gccgaagcga cgcgcggcgc
     1021 tcttcgtcat gggaaactcg gcgcgaggaa ttaatcccgc caagacgtac gaagcaagta
     1081 gattctcttc ttgtttccgc tcgtcgacgc gagcggcgag cgcgaatagc agccaattca
     1141 gttgtgccgc cgggttgcga gttcggctcc gctgcgatat ggtcgctgcg gtggtggtga
     1201 gtcgtgggaa tgcacaaatc tgtaagggtt gttgctttcg taacgctcgt gttcggctgc
     1261 ggtgaccaac aggggctcga gaatggatca ggtagtggct tgtcggaacc gacgagcagc
     1321 ctggaatttg cgttcaatca aggttgcggg aactcggcgc cggcggttgt tggtttcggt
     1381 cacttcgtat acctgtcccc tcaaacctat tccgcgagtg gctgctcgaa gggaatggtc
     1441 atagattgga cggcagggat tggcggaatg ggcgccggcg gtgccacttc ggctagcggc
     1501 ggtatggtca gtgtcggcgg cgctgtcgcg agcagcaaca ccgttgaaat cgactgggcg
     1561 gacgtcctcc caagcaccga agctgcttgc atggactcct ttctggccgc ttaccacttc
     1621 gcggacaacg gcagcggcag ttgggttgca gtcgacttcg cgagcgcaaa cggcacgtgg
     1681 cgtgcgccct ggtgtgtggg cccaagcgtc gtcttttcgg acatcgcgcc cagggcccgc
     1741 atcgcggtta ccgcacgtag caagcaagcc agcacggcgc cgacacgcgc gttcttcatt
     1801 ggtttttcgc cgccatgaaa gtcttgccgg cgagcgtgtc gagtccgctg gctgtcaaga
     1861 gcagcgcgac tgagctgacg tgggcaacgc cggatacgct acttcgaagc ggtcgagcac
     1921 gccgcccgta caccgagcct ccgaactcag gcactttacg gtgaagcgaa ggccgctttg
     1981 gggaggctcc atcacgacgt agttgaacgg ccgccggtcg ccgtcgggtg cgacgtaggc
     2041 cgcggcgtac ggcgcg
//
LOCUS       DASTKS010000735         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1904497, whole genome shotgun sequence.
ACCESSION   DASTKS010000735 DASTKS010000000
VERSION     DASTKS010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904497"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1196
                     /locus_tag="VFQ35_15255"
     CDS             180..1196
                     /locus_tag="VFQ35_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932059.1"
                     /translation="MPRPRSTALCVVRGSNLLATLSFGALLAMTQVAAAQSRAGTSPA
                     QALFDEAKALMQAGRAEEACPKLEESQRLEAGSGTLLNLGLCYEQTGRLASAWSTYIA
                     SEQAARARGKVDRAAAAHERAEALAPQLSRLTIEVPLDARPSGFRIERDGVELDATQW
                     NAAIPLDGGSHSVRASAPGFHEWRESIVVPEKNGLLTLRVPALTPIASPPPTSAPRAE
                     PPRVKLSNSDPSALPPQRVAALVAGGVGVAGVAVGAIYGLRAISKKQQADETCTDGVC
                     TTPGGVRAGKDAHAAGNVATVASVIGVLGLAGGAVLWFTTPHTERSTRVGLGFGSVQL
                     EGRF"
     gene            1196..>3022
                     /locus_tag="VFQ35_15260"
     CDS             1196..>3022
                     /locus_tag="VFQ35_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932060.1"
                     /translation="MRVRSLTRVVAAGTCLFACNALVGVSDDYRLAEGGGAGAGAGRS
                     GGMNAGGKATGGVGASRGGAAGGTEPPENPVSEGGRPTGPPGTGGSSIPDSGGAGGAA
                     GAGPEPVCEDGSAALPSGACVPCGPCVGLGLTGSVHPRTTQTGQCVCQTTEGFFLDGA
                     LSSQPCDADNDGWTRISAKAALESADQALRTNARCTLRSAQEIKLVNEAGQSLSLYFD
                     ATGKNVTATSGGGALPLYESVANDGGTRAGQPALPLYGAGASARSLAPAELNSFTKAC
                     VNSTADHNGNGLADVAEWAQLPAPAASTDPLSEYLPLYTRFSYFTELHTAAFSAPQSR
                     PGGVAGTYTITERKRTGASSDVLVNVVGDPSGGSTYWRSCQRKRDAGYVEGSPAVTYD
                     FASVGSSSASWSGMNHHSQFKCVVGVTSQVYAGFKTEAEHAAALQQQTFDSLSARKWY
                     ANDCRATDSVLPSGADTTNPATPKLDCSPASALNAGDARWVAVSYSAVHGSTSYKYQR
                     GCIDECVDVVQSIPLCQQCDDAAWGQAAVKPVADGVSTDGCKAPLHCDGKGNCGQCNP
                     TAVQCKDKSTAQKCESDRLWHDTACAAGQECLGGNCLKSDGQE"
BASE COUNT          526 a          956 c         1057 g          483 t
ORIGIN      
        1 acgatttgag aggcagagga cgattctgtc ctgcagcgaa tcgcttcgat catcgagctg
       61 atgcgagact ggagaccttg cgtgcggtga gaggtttcgg atagaaaccg gaggcgcttt
      121 gtctcgctcc agctgggtga cgaagcgaca cgatgcttga gccataccga gcccccggtt
      181 tgccgcgacc tcgttcgacg gcgctctgcg tcgttcgggg gagcaacctg ctcgcgacgc
      241 tttcgttcgg cgccctgctg gcgatgactc aggtggccgc cgcgcagtcg agagcgggca
      301 cgagccccgc gcaggccctc ttcgacgagg ccaaggcgct gatgcaagcc ggacgcgccg
      361 aggaagcctg ccccaagctc gaggagagcc aacgtctcga agcgggtagc ggcaccttgt
      421 tgaaccttgg tctctgctac gagcaaacgg ggaggcttgc atccgcctgg tccacgtaca
      481 tcgcgtcgga gcaagcagcg cgagcacgcg gcaaggtcga tcgcgccgca gccgcccacg
      541 aacgcgcgga ggccttggct ccgcaacttt cgcggctcac catcgaggtg ccgctcgacg
      601 ctcgcccctc cgggtttcgc atcgagcgcg acggcgtcga gttggacgcg acccagtgga
      661 acgcagcgat ccccctggac ggcgggagcc acagcgtccg cgcgtccgcg ccgggctttc
      721 acgagtggcg ggaaagcatc gtcgtgccgg aaaaaaatgg gctcctcacc ctccgcgtgc
      781 ccgcgttgac gccgattgcg agccctccac ccacctcggc tccgcgcgcc gaaccgcctc
      841 gggtgaagct ctcgaacagc gatcccagcg cgcttccacc acaacgggtc gcggcactcg
      901 ttgccggtgg cgtcggagtg gcgggcgtcg cggttggcgc gatctatggg cttcgcgcga
      961 tcagcaagaa gcaacaagcc gacgaaacgt gcacggatgg cgtttgcacc actcccggcg
     1021 gagtgcgggc tgggaaggac gcgcacgccg ctggcaacgt ggcgacggtt gcgagcgtca
     1081 tcggtgtgct cggcctggcc ggcggcgccg tactctggtt tacgacgcca cacaccgagc
     1141 gttcgactcg cgtggggctc ggtttcggaa gcgtgcaact ggagggacgc ttctgatgcg
     1201 agttcgaagt ctgactcgag tggtggccgc cggtacttgc ctttttgcgt gcaatgcact
     1261 cgtaggtgtg agcgacgact atcgtctcgc cgagggtggc ggggccggcg cgggagcggg
     1321 gaggagcggg ggcatgaatg cgggcgggaa ggctaccggc ggcgttggag ctagccgagg
     1381 tggcgctgcc ggagggacgg agccacccga gaacccggtc agcgaaggcg ggcgaccgac
     1441 ggggccccct ggcacgggtg ggtcgtctat tccggattcg ggcggagcgg gcggagccgc
     1501 tggcgccggt ccggaacctg tctgcgagga tggttcggcc gcgctcccga gcggagcgtg
     1561 tgtcccctgt ggcccgtgcg tgggactcgg acttaccggc tccgttcatc cgcgcacgac
     1621 ccaaacggga caatgtgttt gccagacgac ggaggggttc ttcctcgacg gcgcgttgtc
     1681 gagccagccc tgcgacgcag acaacgatgg ctggacgcgt atttcggcaa aggctgcgct
     1741 cgagagcgcg gaccaggcgc tgcgcaccaa tgcgcgttgc acgctgcgtt cggcgcagga
     1801 aatcaagctc gtcaacgaag ccggtcagtc gctgtcgctg tacttcgacg cgacggggaa
     1861 aaatgtcacg gctacgagcg ggggtggcgc gctgccgctc tacgagtccg tcgcgaacga
     1921 tggcggaacg cgcgctggcc aaccggcgct gccactttat ggcgccggcg cgagcgcgcg
     1981 ctcgctggcg cccgcggagc tcaacagctt cacgaaggcg tgtgtcaatt cgaccgcaga
     2041 tcacaatggt aacggcctcg ccgacgtagc ggagtgggcg cagctgcccg cgccggcggc
     2101 gagcactgat ccgctctcgg aatatctccc gctgtacacg cgcttcagct acttcaccga
     2161 gctacacacg gcggcgttct ccgccccgca gtctcgtccg ggtggtgtcg cagggaccta
     2221 cacgatcacc gagcgaaagc gcacgggcgc cagcagcgat gtgctcgtca acgtcgtcgg
     2281 cgaccccagt gggggaagta cgtactggcg cagctgtcag cgcaagcgcg atgcgggcta
     2341 tgtcgagggg agcccggccg tgacctacga cttcgcttct gtcggctcgt cgagcgcgag
     2401 ctggtcgggg atgaaccatc acagtcagtt caagtgcgtc gtcggcgtca cgtcgcaggt
     2461 ttatgcgggg ttcaagaccg aggcggagca cgccgcggcg ttgcagcaac agaccttcga
     2521 cagcctgagc gcccgcaagt ggtatgcgaa cgactgccgg gcaaccgaca gcgttctccc
     2581 gagcggcgct gacactacga accccgcgac gccaaagctc gactgttcgc ccgcgagcgc
     2641 gctgaatgcc ggcgacgctc gttgggtcgc ggtgagctat agcgcggtcc acggcagcac
     2701 gtcgtacaag taccaacgtg gttgcatcga cgaatgcgtc gatgttgtgc agtcaatacc
     2761 tctatgccag cagtgcgacg acgcagcctg gggccaagcg gcggtcaagc ccgtcgcgga
     2821 cggcgtctcc accgacggtt gcaaggcgcc tttgcattgc gacggcaaag ggaactgcgg
     2881 gcaatgcaat ccgaccgcgg ttcaatgcaa agacaagtct accgcgcaaa agtgcgagag
     2941 cgatcggctc tggcacgaca cagcttgcgc tgcggggcag gagtgcctgg gaggcaactg
     3001 cctgaaatcc gacggccaag ag
//
LOCUS       DASTKS010000736         6610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1458147, whole genome shotgun sequence.
ACCESSION   DASTKS010000736 DASTKS010000000
VERSION     DASTKS010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458147"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFQ35_15265"
     CDS             <1..1415
                     /locus_tag="VFQ35_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9932061.1"
                     /translation="ALPSAELDGALLDRALESAGCRVVIAEVTLADRLGSRRRCIRYG
                     DEAWRQMVAESASLPRTPLRCESNADFAYVFSSGTTGVSKACRVSHWRSRLSATAFSR
                     LVHGLRADDVLYCPLPLYHASPLLLGFGACLVAGATLALRSRFSASAVLADVRRFDAT
                     VMLYVGELGRAWLAQPPSPHDRAHRLRALVGNGMNARVWAALRERFAIPHVREFYAAS
                     EFPGAVVNITDQVGSVGHLPFGRLRGYRLVRVDANDGSIVRDASGRAIECAADEPGEL
                     VLRLRPSRERPTGEYLGYVGEGPNAERIARDLFEPGDLYCRSGDLLRRDRAGHFYFVD
                     RLGDTFRFKSENVSTREVEDAFSALPGVVSAVSLGVRLPEFDGKLGLLALEVGAEFSL
                     ASVAERMVTLPRAMRPCFVRLVEHIELTPSLKYKKSDIARDGVDPSVIGDPLFFRDGE
                     ELRPLDPAAFAGILGGAHRF"
     gene            1473..2225
                     /locus_tag="VFQ35_15270"
     CDS             1473..2225
                     /locus_tag="VFQ35_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932062.1"
                     /translation="MRFRQLCIAALMAASTFACGAKRRPAKGALPPLTASSWLIGLPN
                     AESGEAKVAVPLGAVKARPLLIALHGEVDRPEWQCGSYRGITHGRSFILCPSGPARAA
                     LGGVRAPVADTARELRAEIAALKAKFGAHLARGSVVLAALGPTVEQALTIALEEPSFF
                     SRLVLVNGSLSRLDAAFATRYGQLGGERVLVVCSRGACDADADTRARSLRPSGVEVHF
                     VVPERGEGLDGEIARVIEEQWPWLVGSDSRWR"
     gene            2186..3055
                     /locus_tag="VFQ35_15275"
     CDS             2186..3055
                     /locus_tag="VFQ35_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932063.1"
                     /translation="MALARWLRLSLALMPFASACARAEAGANGREPRPLSPVESAEPK
                     RNDATPPLAEPARVEPSPPRLVSAPLVELEVPGFRPATLVVPAGAQSKRPIVVALHGN
                     FDRPEWQCGVWQTIVEGNAFVLCPRGIPRRDAPAALDRWEYASAKAMKSELEAGLAAL
                     RARFADFVDDGPIVFIGFSLGAIYGASLVHTEPARFSRVVFIEGGVGTWTGASAKKFS
                     QAGGQRLILACGQADCLSRVKRLGPSLEKAGLPTRVGGSAKAGHTYDGEVADVVRENW
                     SWFVEGDARWAHP"
     gene            3286..4641
                     /locus_tag="VFQ35_15280"
     CDS             3286..4641
                     /locus_tag="VFQ35_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET9932064.1"
                     /translation="MSFLAGLACAGGLLLIMGLAAGYLKRLPISTSAIYLLLGLAIGP
                     VGFNWLVFSLRGSAEWMERLCEIAVAVSLFIGGLKMRLPLRHPAWSPAYILAGPVMLV
                     TVAGVALFARASLGLAAADCLLLGSILAPTDPVLASAVSVNDARDQDRMRYGLSGEAG
                     FNDGIAMPLVMLALLWREHASVGGWVWPWVLQHVAWAMPAGCAIGFLMGSGIGQLAVR
                     LRSRLQEPHAPSDFLALALILLSYVAAEAVSALGFLAVFAAGVGLRHAERKIVAASPH
                     PNAPRTDGRKTHPPAEDLATTRIAEEQAGEPAVAVGVLVAETTLFGDTVERLLEVMLV
                     LLVGASLVHAFSLKGVLVALFLFLLLRPLATLALLAHTETTWRQRWLMGWFGIRGIGS
                     LYYVSYALNRGVSDPAAHQLTSVTLSVIATSILLHGVSAQPLLTYYEKALKRRRERRP
                     A"
     gene            complement(4775..5311)
                     /locus_tag="VFQ35_15285"
     CDS             complement(4775..5311)
                     /locus_tag="VFQ35_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932065.1"
                     /translation="MTTGNELLRVQIPAPSGSFALRATVTAGPSAAYLRTPLDPIQTE
                     GSLFLSGWVRVPSEAAHNVAPFAFWSANEEDWALRVAIQDARLDVWTKTTPVTGSFQL
                     QRGEWYCLGLELTVGNAPDGRVTITVNDQEVAGATELDTLPDGGIQAVAVGTLWTNSP
                     AEVMVDRVVVSRERVPCF"
     gene            complement(5809..>6610)
                     /locus_tag="VFQ35_15290"
     CDS             complement(5809..>6610)
                     /locus_tag="VFQ35_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932066.1"
                     /translation="MFGLALRGYQRRVQRLNESATDRGKTLWQAVLELLEKEGAVTRR
                     RVFQRFQNDDAALVGAVLNDLTATGLTFRTGSGDDTVYGLTQERERVALARAGSLETI
                     ASLVWLEFCRDPRATTREVAERLALGDDQVEAALVSLEEDGRVRRQDGRILAEALTIP
                     VGAHAGWEVAVFDHFRMVCVALATKLNRGEARAETTDTTGGQTLTFELDDEHPARAEV
                     LGLLARLRAETNDLWQRVEDHNRAVPKVDRHRVGFYMGQVEMPRDDEA"
BASE COUNT         1019 a         2159 c         2174 g         1258 t
ORIGIN      
        1 ctgctttgcc gagcgccgaa ctagacggag cgttgctcga ccgtgctctc gaaagcgcgg
       61 gctgtcgagt cgtcattgcc gaggtgacgc tcgccgatcg gttggggtcg cgcaggcgct
      121 gcattcgtta cggagacgag gcgtggcgac agatggtcgc ggagagcgct tctttgcctc
      181 gtacgccgct tcgctgcgaa agcaacgccg acttcgctta cgtcttcagt tcgggaacga
      241 cgggcgtctc gaaagcgtgc cgggtgagcc attggcgctc gcgcctctcc gcgactgctt
      301 tctctcgcct cgtgcacggc ctgcgcgcgg acgatgtcct ctattgcccg ctgcctctct
      361 atcacgcaag ccccctactg cttggcttcg gcgcgtgcct ggtggcgggg gcgacgctcg
      421 ctttgcgatc tcgcttcagc gcgagcgcgg tgctcgccga cgtgagacgt ttcgatgcga
      481 cggtcatgct ctacgtgggt gagctcgggc gcgcttggct cgctcaaccg ccgtcacctc
      541 acgatcgcgc gcatcgcttg cgagcgctgg tgggcaacgg catgaacgcg cgagtttggg
      601 ccgcgttgag ggagcgcttc gcgatccctc acgtccgaga gttttacgcc gcgagcgagt
      661 ttccgggcgc ggtcgtcaac attacggacc aggtcggcag cgtcggccac ttgccgttcg
      721 ggcgcttgcg cggctatcgg ctcgtgcgcg tggacgcaaa cgacgggagc atcgtgcgcg
      781 acgcctcggg gcgagccatc gaatgcgccg ccgacgagcc gggcgagctc gtcctgcgcc
      841 tacgaccgtc gcgtgaacga ccgacgggcg agtacctggg ctacgttggc gaaggtccga
      901 acgccgagcg catcgctcgc gatctcttcg agccgggcga tctgtattgt cgctcgggag
      961 atttgctacg gcgagatcgc gccgggcact tttacttcgt cgatcggctc ggcgacacct
     1021 ttcgcttcaa gagcgaaaac gtctccacgc gcgaggtcga ggacgccttt tcggcgctac
     1081 ccggcgtcgt atccgcggtg tcactcgggg tgcggttgcc tgagttcgac ggcaagctcg
     1141 gcctgctggc tctggaggtc ggcgccgaat tctccctcgc gagcgtcgcc gagcgcatgg
     1201 tgaccctgcc gcgcgccatg cggccgtgct tcgtccggct agtggagcac atcgagctca
     1261 ccccttccct caagtacaaa aagtcggaca tcgcccgaga cggagtcgac ccgagcgtca
     1321 tcggagatcc tctgttcttt cgggacggcg aagaacttcg cccgctcgat cccgccgcct
     1381 ttgccggaat tctcgggggc gcgcaccgct tctaatccgc agctgcccac aaagcttgtc
     1441 ccgggcggcg ccgctcgtgg acaatcagcc agatgcgctt ccgacagctc tgcatcgccg
     1501 ccctcatggc ggcttctacc ttcgcctgcg gtgcgaagcg tcggcctgcg aagggagcgc
     1561 taccgccgct cactgcatct tcgtggctca tcgggctgcc gaatgcggag tccggtgaag
     1621 ccaaggtggc cgttccactc ggcgccgtaa aggcacgccc gctgctcatc gcgctgcacg
     1681 gcgaggtcga tcggcccgaa tggcagtgcg ggagctaccg aggcatcacc cacggtcggt
     1741 cgttcatcct ttgtccgagc ggccccgctc gcgccgcgct gggtggggta cgcgctccgg
     1801 tagcggacac cgctcgcgag ctccgcgccg agatcgcggc cctgaaagca aagtttggcg
     1861 cgcatctcgc tcgcggttca gtcgttctcg ccgcgctcgg tccaaccgtc gagcaagcac
     1921 tcacgatcgc gctcgaagag ccgagcttct tctcgcggct cgtgctcgtg aacggctcgc
     1981 tatcccgcct cgatgcggcg tttgcgacac gttacggtca acttggcgga gaacgcgtcc
     2041 tcgtggtgtg cagccggggc gcttgtgacg ccgacgcgga tacgagagca cggtccctgc
     2101 gcccatcggg cgtggaggtg catttcgtcg tgcccgagcg tggcgagggg ctggacggcg
     2161 agatagcgcg cgttatcgaa gaacaatggc cttggctcgt tggctccgac tctcgctggc
     2221 gctaatgccc ttcgcttcgg cgtgcgctcg cgctgaagcc ggtgcaaacg gccgcgagcc
     2281 gaggccgctc tccccggtcg aaagcgcgga gccgaagcgc aacgacgcga cgccgccgct
     2341 cgctgaacct gctcgggtcg agccgtcgcc gccacgactc gtgagcgcgc cgctggtgga
     2401 gctcgaagtt ccgggttttc gtcccgcgac cctcgtcgtc cctgctggcg cgcagagcaa
     2461 gcgcccaatc gtcgtcgccc tgcacggaaa cttcgatcga ccggagtggc aatgtggagt
     2521 ttggcagacc atcgtcgagg gtaacgcctt cgtcctttgc ccgcggggga tcccacggcg
     2581 tgatgcgccc gcggcgttgg atcgctggga atacgcttca gccaaggcga tgaagagcga
     2641 gctcgaggcg gggctcgccg cgcttcgtgc ccgctttgcc gacttcgtcg acgatgggcc
     2701 gatcgtcttc atcgggttct cgctcggggc gatttacggc gcgtcgctgg ttcacaccga
     2761 gcccgcgcgc ttttcgcgcg tggtgttcat cgagggcggc gttggcacgt ggacaggagc
     2821 gagcgcgaag aaattctccc aagccggcgg tcagcggctc attcttgcgt gcggtcaggc
     2881 agactgcttg agccgcgtaa aacggttagg tccgagcctc gagaaagccg gtttgccgac
     2941 gcgcgtcggt ggcagcgcca aggcgggcca cacttacgat ggggaagtcg ccgacgtcgt
     3001 gcgtgaaaac tggagctggt tcgtagaagg cgatgcgcgt tgggcgcatc cgtgacactt
     3061 gcgcgttgcg gcagtcgaca cgagtattgc gcgatggccg ttgctcctcg ttggcttggc
     3121 cggatctgtg gcgttcacga acttcacgcc gaagcgcccc atgtcccgca gatgcgatag
     3181 cgaatcgcgc taacacgccg ggcggcgcgc gcggcgcggc atgaggcgtg caggcattcg
     3241 ggcgatgctg gcgaaccaag gggagcgctg cggcagcgcg aacccatgag cttccttgca
     3301 ggcttggcct gcgctggagg cttgctcttg attatggggc tcgcggctgg gtacttgaaa
     3361 cgcctcccga tttcgacctc agccatttat ctgttgcttg gtttggcgat cggtcccgtg
     3421 gggttcaatt ggctggtgtt ctcgctgcgc ggttcggcgg aatggatgga acggctgtgc
     3481 gagatcgccg tagccgtatc gttgttcatc ggcggtctga agatgcgctt gcccttgcgt
     3541 catcctgcgt ggtcgcccgc atacatcctc gcgggcccgg tgatgctggt cacggtcgcc
     3601 ggggtcgcgc tctttgcgcg ggcttcgctg gggctggccg cggcggattg cctgttgctc
     3661 ggctccattc tggctccgac tgatcccgtg ctggcgagcg cagtctcggt caacgacgct
     3721 cgagaccaag atcgcatgcg ctatggattg tcgggcgaag ccgggttcaa cgatggcatc
     3781 gcgatgccgc tcgtaatgct cgccctgctc tggcgagagc acgcttcggt cggaggatgg
     3841 gtgtggcctt gggttctcca gcacgtcgcc tgggccatgc ccgctggctg cgcgatcggc
     3901 ttcctcatgg ggagcggcat cgggcagctc gcggtgcggt tgcggagccg cctccaggag
     3961 ccccacgcgc cgagcgactt cctcgcgcta gcgctgatcc tgctttcgta cgtcgcagca
     4021 gaagccgttt ccgcgctcgg gtttctcgct gtcttcgcgg cgggagtggg tttgcgccat
     4081 gcagagcgga agatcgtcgc cgcaagccct catccgaatg cacctcgtac ggatgggcga
     4141 aagacgcacc cacccgccga agatctcgcg actacgcgaa tcgcggaaga acaagcggga
     4201 gaacctgcgg tggcagtcgg agtgttggtc gccgaaacga cgctgtttgg cgacacggtc
     4261 gagcgtctgt tggaagtgat gttggtcctc ctcgtcggag cctcgctcgt ccacgccttc
     4321 tcgctgaaag gcgtgctcgt ggcactattt ctatttctgc tgctccggcc cctggcgacg
     4381 ctagctctac tcgctcacac ggagacgacg tggcggcagc gctggctcat gggttggttt
     4441 ggcattcgcg ggatcggcag tctttactac gtcagctacg ctttgaatcg cggcgtgtca
     4501 gaccctgcag cgcatcagct aacgtccgta acactctccg tgattgcgac gagcatcctt
     4561 cttcacggcg tgagtgcaca gccacttttg acctactacg agaaggcgct gaagagacgt
     4621 cgtgagcgca gacccgcgtg aagacgaccc cgctgccggc acggcgagga tcttcgagcc
     4681 gtagcgctgc acgtcgctag aagagacgca gcgtggcgtg agtgcgcgtc agcgcgcgct
     4741 cgagggccgc tcgttcgccg cgaagtcacg acctctagaa gcagggcacg cgctcgcgac
     4801 ttaccacgac ccggtcgacc atgacttcag cgggcgagtt ggtccacaag gtgcccaccg
     4861 cgacggcttg gatcccgcca tcgggtaggg tgtcgagttc ggttgcgccc gcgacctcct
     4921 ggtcgttcac ggtgatggtg acacgtccgt ctggtgcgtt gccgacggtg agctcgagcc
     4981 caaggcagta ccactcgccg cgctgaagct ggaaggagcc ggtcacgggt gtcgtcttgg
     5041 tccagacgtc gaggcgcgcg tcctgaatcg cgactcgcaa cgcccaatcc tcttcgttcg
     5101 ccgaccagaa cgcgaacgga gcgacgttgt gagcagcttc gctcgggacg cgcacccaac
     5161 cgctcaagaa caacgagccc tccgtttgta ttgggtcgag cggcgttcgc aggtacgccg
     5221 cggaggggcc cgccgtaacg gtagcgcgga gcgcgaaaga gcccgaagga gccggaatct
     5281 gcacccgcaa cagctcgttg cctgtcgtca cgacgggagt tgcgctcgtc gagtcggtct
     5341 cgaagtcgtc gcagtaaaga gccccgtctg ggcaccggtt tggcagcggg tcgacgcaag
     5401 cgcccgccag acatcggcgc ccgctttcgc actctgcatc acccttgcaa gcgggctggc
     5461 aagtcgcaag tgcttctccc ccgcactgca cgaggctgga gagcccgccg gtgcaggtga
     5521 gcccatcgat gcaagcattc gaggactcgc aggttaccgt gcaacgaccg cacaagcacc
     5581 gagccgctct tccgcactgc gcgtcgttct cgcagcgacg cagccaattc gtattcgtgc
     5641 cggagtcttc ttcaggtgag ccacaagcac tcgctgcgat cagggtcgcg agggcaaccg
     5701 ctgccaaccc gcgcgcagac gctgcgagcc gctcccgaac actcggctgt gacgatgcca
     5761 accaatcaag cggcctccgg cgccgtgcgg ctgacgcccc tcgcctcgtc atgcctcgtc
     5821 atccctcggc atctcgacct gccccatgta gaagccgacg cgatggcggt ctaccttcgg
     5881 gacggcgcgg ttgtggtctt caactcgctg ccacaagtcg ttcgtttcgg cgcgcaaacg
     5941 cgccagtagc ccgaggacct cggcacgcgc gggatgctcg tcgtccagct cgaaggtcaa
     6001 ggtttgaccg ccggtggtgt ccgtcgtctc tgcacgcgcc tcgccgcgat tcagcttggt
     6061 cgcgagcgcc acgcaaacca tccgaaaatg atcgaagacg gctacttccc accccgcgtg
     6121 ggcccctacc ggtatggtca atgcttctgc caagatccgg ccgtcctgtc gacgcactct
     6181 gccatcttct tccagcgaaa cgagtgcagc ttcgacctgg tcgtcgccga gcgcaagcct
     6241 ctcggccacc tctcgcgtcg tcgcgcgtgg atcgcggcag aactcgagcc aaacgagtga
     6301 tgcgatggtc tcgagagagc cggcccgcgc gagtgccacg cgttcccgtt cttgggtcag
     6361 gccatacacg gtgtcgtctc cgctgccggt acgaaacgtg agccccgtcg cggtgagatc
     6421 attcagcacc gctcccacga gcgcggcgtc gtcgttctga aaccgttgaa agacgcgacg
     6481 acgagtgacc gcgccctcct tttcgaggag ctcgagcacc gcttgccata gggtctttcc
     6541 ccgatccgtc gcgctctcgt tcaggcgctg cacgcgacgc tgatagccgc gcaacgcgag
     6601 gccgaacatg
//
LOCUS       DASTKS010000737         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1680559, whole genome shotgun sequence.
ACCESSION   DASTKS010000737 DASTKS010000000
VERSION     DASTKS010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680559"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1288
                     /locus_tag="VFQ35_15295"
     CDS             176..1288
                     /locus_tag="VFQ35_15295"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9932067.1"
                     /translation="MNPRAESRAGLSRVIESERPTYLLGLVRVAVGVFLLVGTFFAIR
                     EVLDHGYFADAFFIPVLPHAAVASRSVYLALLGLRGVASLLIVIGVQARPALLVASSI
                     GLYGLLCDRLEYHNNRYVLELLAFLLAFAPCDRSFVLRKGNGGANGPVWAIWLMKLQI
                     SLVYAASAGSKLVDTDWRGGQVLHIRYLHVLDLAAARGKELPNWVHTALSSPFFAELS
                     SKAAIFGELFLAVGLWVARTRPFALWLGVMLHLGIQLAAHVEIFSFLMGAAYVLFARQ
                     ELGARTLHFDPASLRGARIGAVVQRLDWLKRFTIEPRAGSFEIVERDGTSAHGFDAIV
                     ALTRALPLLFPLWLPLAIAARLRGGGVRVAPAARAP"
     gene            1374..1856
                     /locus_tag="VFQ35_15300"
     CDS             1374..1856
                     /locus_tag="VFQ35_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9932068.1"
                     /translation="MSNDLILRRAAKADLPTVGEFAGRLVRDHHEADPARFFLPERVE
                     QGYAWWLGQELENPKALVMVAELSGELVGYSYSEVSERDWNLFIDAHATLHDILVAPE
                     VRRRGVGRELLAATLRELEARGVTRILLYAREGNEKAERLFASVGFRRTMIEMMRATP
                     "
     gene            complement(1859..2794)
                     /locus_tag="VFQ35_15305"
     CDS             complement(1859..2794)
                     /locus_tag="VFQ35_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9932069.1"
                     /translation="MNEKTSVPPADKDRAMLALERAVACAERGISDRPVVLTSPVDLA
                     ELPRGDVRSRLDRVMMGDHPDEGLDALLVSGILDALLPEVKAMVGFGDGEWRHKDVWK
                     HTKQVVAQSVPRIEVRYGALFHDIGKVRTRSISPDGEVHFFGHAEVGARMFDKLERRE
                     RLFSSEESLRKDVRFLVLHHLRASQYDGKWTESAVRRFAREMGAHLEDLLCLSRADIT
                     TKRPEKKRRGISLINELAERITELAALDAVQPALPTGVGNAIMEAFKLPPSRLIGDIK
                     RALEGAVDAGEIEARLECEAYVQFVAQNKSRFGLE"
     gene            2959..>3782
                     /locus_tag="VFQ35_15310"
     CDS             2959..>3782
                     /locus_tag="VFQ35_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932070.1"
                     /translation="MVGRPSPAARRFDAARLLARILCAVFGLVGVLPLAAGFFVELGR
                     VQRWAAAETARVLKEQLGVQASFEVHLHIVPLRLRVEHLVVPASDGGTPALTVERVSV
                     TPRLFSLLAGRLDIGDVEVESPRARLVIEKGEIKNLRYRLPKTQTKPSKTTSSPFGSL
                     SLNDGRFTVDIDGTRIDTGTLDLDVFAEAGPVFEVMLSGSGATIARSRRPIGSGIPAD
                     APLVHDADALCRLELRARIENGELLIRRLSVLGVADLDPEQGAAPSCEIGSNAVGRV"
BASE COUNT          572 a         1279 c         1231 g          700 t
ORIGIN      
        1 agttcgtaca cgccacgtac ggcctcgacg ctcaactgtt tcggctgcaa ctcgcgctcg
       61 aggacgtcgc agcacacatc caggaggacc gtgaaacggt cgcgctcgta gcaaaggtcg
      121 agacactcaa gaacggcaga cctggcgaaa accgaacact ggaggcgcgg cgaccgtgaa
      181 cccccgggca gagtcgagag ctggcctgag ccgcgtcatc gagtccgagc ggcccaccta
      241 cctgctcggg ctggtgaggg tcgccgtggg cgtcttcttg ctggtgggga ccttcttcgc
      301 gattcgcgaa gtcctcgacc acggttactt cgccgatgcc ttcttcatcc cggtgctgcc
      361 ccacgccgcc gtcgcgtcgc gctcggttta cctcgcgttg ctcgggctgc gcggcgtcgc
      421 gtcactcctc atcgttatcg gcgttcaggc tcggccggcg ctgctcgtcg cttcgtcgat
      481 tggcctctac gggctgctct gtgaccgact cgagtaccac aacaaccgct acgtcctcga
      541 gctcctcgcg ttcctgctcg cgttcgcacc gtgcgaccgt tccttcgtgt tgcgaaaggg
      601 gaacggcggc gcgaacggcc ccgtctgggc gatctggttg atgaagcttc agatctcgct
      661 cgtgtacgcc gcctcggccg ggagcaagct cgtcgacacc gattggcgcg ggggccaggt
      721 gctgcatatc cggtacctgc acgtcttgga tctcgcggcc gcgcgcggga aagaactgcc
      781 gaactgggtg catacggccc taagctcgcc gtttttcgca gagctctcgt cgaaggcggc
      841 aatcttcggc gagctcttcc tcgcggttgg gctttgggta gcgcgcacgc gcccgttcgc
      901 gctctggctc ggcgtgatgc tgcatctcgg gatccagctc gccgcgcacg tggagatttt
      961 cagcttcttg atgggcgccg cgtacgtgct cttcgcgcga caagagctcg gcgcgcgcac
     1021 cctccatttc gatcccgcat cgttacgcgg cgcacgcatc ggagccgtcg tgcagcgact
     1081 cgactggctc aaaaggttca ccatcgaacc ccgcgccggc tcgttcgaga tcgtcgaacg
     1141 tgatggcact tcggctcacg gcttcgatgc gatcgtcgcg ttgacccgcg cgctgccgct
     1201 cctctttccg ttgtggctcc cgctcgcgat cgcggcaagg ctccgcggcg gtggggtccg
     1261 cgtcgcgccg gccgcgcgcg ctccgtgacg cgcgtcgtcc ccgtgcctcc accgccgcac
     1321 ggcgcgaggc ccgccgggtc gccatcgcgc cccttcagcg tatgctctct cccatgtcga
     1381 acgacctgat tttacgccgc gccgcaaagg cagatctgcc cacggtcggc gaatttgcag
     1441 ggcgcctcgt gagagaccac cacgaggcgg atccagcgcg cttcttctta ccggagcggg
     1501 tcgagcaggg ctacgcctgg tggctcggtc aggagctcga gaatccaaag gcattggtga
     1561 tggtcgcgga gctctcgggg gagctcgttg gatacagcta ttcggaggtg agcgagcgcg
     1621 actggaacct gttcatcgac gcgcacgcga cgttgcacga catcctcgtg gctcccgagg
     1681 tgcgacggcg cggtgtgggc cgagagctcc tggcggcgac gctgcgtgag ctcgaagcgc
     1741 gcggcgtgac gcgcatattg ctctacgcgc gtgagggcaa cgaaaaagcc gagcgcctat
     1801 tcgcctcggt tggttttcgg cgcaccatga tcgagatgat gcgcgcgacg ccgtagccct
     1861 attcgagccc gaagcgcgac ttgttttgcg cgacgaactg gacgtacgcc tcgcactcga
     1921 gccgcgcttc gatctcaccg gcgtcgacgg cgccttccag ggcgcgcttg atgtcgccga
     1981 tgaggcgtga cggaggcaac ttgaaagcct ccatgatcgc gttgccgacc cccgtgggca
     2041 gcgccggctg aaccgcgtcg agggcggcga gctcggtgat gcgctcggcc agctcgttga
     2101 tgagcgagat gccgcgacgc ttcttctccg ggcgcttggt cgtgatgtcg gcgcgcgaaa
     2161 ggcagagcag atcttcgagg tgcgcgccca tttcacgggc gaagcgccgc acggcgctct
     2221 ccgtccattt gccgtcgtat tggctcgcgc gcaggtggtg caaaaccaag aagcgcacgt
     2281 ccttgcgaag cgactcctcg ctgctgaaaa ggcgctcgcg ccgctcgagt ttgtcgaaca
     2341 tgcgcgcgcc gacctcggcg tgaccgaaga agtgcacctc gccgtcgggg ctgatgctgc
     2401 gcgtgcgcac cttgccgatg tcgtggaaca gcgcgccgta acggacctcg atgcgaggca
     2461 cgctctgtgc gaccacctgc ttcgtgtgct tccacacgtc cttgtgccgc cactcgccgt
     2521 ccccgaaccc gaccatcgcc tttacctcgg gcagtagcgc gtcgaggatg ccgctcacga
     2581 gcaacgcatc caacccctcg tcggggtggt ctcccatcat cacgcggtcg agtcgcgaac
     2641 gtacgtctcc gcgcggaagc tccgcgagat ctaccggcga agtcagtacg actggccgat
     2701 ccgagatccc gcgctccgcg caggccaccg cccgctcgag agcgagcatg gcgcggtcct
     2761 tatcggccgg ggggacgctc gtcttctcgt tcatggctgc gcgggccggc tcttctacgt
     2821 gcccgccccg aaagcgcaag cagaaagcga cgccggttct gtacgcctcg gaactttcct
     2881 cctggtttgc gcggagcgcc gcccaaattc agcagaaacc gggcactttt cggttttgag
     2941 cgatacgctt cagcccagat ggttggtcgc ccgagcccgg ccgcccgccg cttcgacgcg
     3001 gcacggctgc tcgcccgaat tctctgcgca gttttcgggc tggtcggcgt gttgccactg
     3061 gcggccggct ttttcgtaga gctcgggcgt gtccagcgct gggcggcggc ggaaaccgcc
     3121 cgcgttttga aggagcagct cggagtccag gcaagcttcg aggttcacct tcacatcgtt
     3181 ccgctccggc tgcgggtcga gcacttggtc gtgccggcgt cggacggcgg aacccccgcg
     3241 ctcacggtcg agcgcgtcag cgtgacgccg cggctattca gcctcctcgc ggggcgcctc
     3301 gacatcggcg acgtggaggt cgagtcgccg cgcgcccgcc tcgtcatcga aaaaggcgag
     3361 atcaagaacc tgcgctatcg gcttccaaag actcagacga agccgtccaa gacgacgagc
     3421 agccccttcg gctcgctatc gctgaacgac gggcgcttta cggtggacat cgatggcacc
     3481 cgcatcgaca ccggcacgct cgacctcgac gtgttcgcgg aagcagggcc agtgttcgag
     3541 gtgatgctga gcggcagcgg cgcgaccatc gcgcgctcgc gaaggccgat tggctccggg
     3601 atccccgccg acgcgccgct ggttcacgac gcggacgcgc tctgccggct cgagcttcgc
     3661 gcgcgcatcg aaaacggcga gctcttgatt cgtcgattgt ccgtactcgg agtcgccgat
     3721 ctggatcccg agcagggcgc tgcgccttcc tgtgagatcg ggagcaacgc cgtcggtcga
     3781 gt
//
LOCUS       DASTKS010000738         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_564347, whole genome shotgun sequence.
ACCESSION   DASTKS010000738 DASTKS010000000
VERSION     DASTKS010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564347"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFQ35_15315"
     CDS             <1..1003
                     /locus_tag="VFQ35_15315"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9932071.1"
                     /translation="EPHPRSKSDAVTSSERAPSEAGAKGPSLVVIGNFDGVHRGHRAV
                     IASALSLAKAHGLAPKVLTFHPHPSEVLGRGVRPVLTPVERKVELLQRLGAELEVVVA
                     PFTLELSRMTPRDFVRHVLVEELVAKVVIVGENFRFGHGRAGDLHTLRELGAEFGFEA
                     RAEPLVGDELGIFSSTRAREALERGDLSGVLRCLGRPHALSGTVVHGAHRGRDLGFRT
                     ANLGEVREAIPPYGVYACLVDRLDGGNARALARGVMNLGVRPTVQAGYSAEVHLLDWE
                     GDLYGATLRVHLVDRLRGEQRFPSLEALVEQIHRDASAARELLEGRAPDPAANGAWA"
     gene            1071..2597
                     /locus_tag="VFQ35_15320"
     CDS             1071..2597
                     /locus_tag="VFQ35_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932072.1"
                     /translation="MDPANFDKMSRDELVAKARSLGVERPELMTRVELGDEIVRRTQT
                     DPGAQQRARGWLGVARDLVASVVGSGLSMPDAAALIRGDRSVLELKGPSPVATVTLAE
                     IYATQGHTERALSMLDEVLTREPEHAAALALRARLLGEPRTRRVDAAVGQPKSNAEPQ
                     VWAASATIEEDDAPRTSQTPPDEEAELPSATPELVGEAAPREVPPVHAASDSDAGVVD
                     ADAPAFEPVSLAPPLTDLPTRPDATALAAPVHPPAEREPEPSPVQTYPEPAPEPPAPE
                     ATPEPAMESPAPVSDAIPTPASPELRVAPAEPEPASGVEVPAEPPGPDEFAAEIQAAP
                     EPARFGLEPVEPSASLEPVQELAPEPSQPQSDIVPTLLPAPMPEPEPAPALIVLRRSS
                     QDPLVCWDLPESQLAAHSPLELDCHGFSASPKGTERRSVTVLVESRRGSAVLSGFARA
                     TLIRVALGTRSEGSFLPLVIASELRQSDVSLDVSFRPPLSESTPPTPIERGLIDQFAN
                     "
BASE COUNT          423 a          970 c          965 g          472 t
ORIGIN      
        1 ggagccccat ccccggagta agagcgacgc cgtgacgtcc tccgagcgag cgccgagtga
       61 agccggggcg aagggtccgt ccctcgtcgt catcgggaac ttcgacggcg tacaccgcgg
      121 gcatcgggcc gtgattgcgt cagcactgtc gctcgccaaa gcgcacggcc tcgcgccgaa
      181 ggtgctcacc tttcacccgc atccatccga agtgttgggg cgtggcgtgc gcccggtgct
      241 gactcctgtc gagcgaaagg tggagctgct gcagcggctc ggcgcagagc tcgaggtggt
      301 cgtggcgccc tttacgctcg agctttcgcg catgacaccg cgcgacttcg tacgacacgt
      361 gctggtcgaa gagctggtcg cgaaggtcgt catcgtgggc gagaattttc ggttcggcca
      421 cggacgcgcc ggcgatcttc acacgctgcg cgaactcggt gccgaattcg ggttcgaggc
      481 gcgcgcggag cccttggtgg gagatgagct cggcatcttc tcgtctacca gggctcgcga
      541 agccctcgag cgcggcgacc tcagcggcgt tcttcgttgt ctcgggcggc cgcacgccct
      601 gtcggggacg gtcgttcacg gcgctcatcg ggggcgcgac ttgggtttta gaacggcgaa
      661 cctcggcgag gtgcgcgaag ccattccgcc gtacggggtc tacgcatgcc tcgtcgatcg
      721 gttggacgga ggaaatgcgc gggcgctcgc gcgtggcgtc atgaacctcg gcgttcgtcc
      781 cacggtgcaa gcgggataca gcgcggaggt tcacctgctc gactgggaag gtgacctcta
      841 cggcgcaacg ctccgagtgc acttggtcga tcggttgagg ggggagcagc ggtttccgag
      901 cctcgaagcg ctcgtcgaac agattcatcg ggatgcgtcg gcggcgcggg agcttctcga
      961 ggggcgcgcg cccgatcccg cggccaacgg cgcttgggca tagttttcgg ccccttggcc
     1021 cgaagcttgg cttgaccgcg agcgcggggc ggcttctgaa tagcgcccag gtggaccccg
     1081 caaacttcga caagatgagc cgcgatgaac tcgtggcgaa ggcgcgctcg ctcggcgtcg
     1141 aacgtccaga actcatgact cgcgtcgagc tcggcgacga gatcgtgcgc cgcacgcaga
     1201 cggaccccgg agcgcaacag cgcgcgcgtg gttggctggg cgttgcgcgc gatttggtag
     1261 cgagcgtcgt gggatcgggg ctgagcatgc cggacgcggc cgcgctcatc cgcggtgacc
     1321 gcagcgtgct cgagttgaag gggccgagcc ccgtcgcgac cgtgacgctc gccgagattt
     1381 acgcgacgca ggggcacacc gagcgcgcgc tcagcatgct cgacgaggtg ctgacgcgcg
     1441 agcccgagca cgctgctgcg cttgcgctgc gcgcgcgttt gctcggcgag ccgcgcacgc
     1501 gccgcgtcga tgcggccgtc ggtcagccga agagcaacgc ggagcctcag gtctgggccg
     1561 cgtctgcgac catcgaggaa gacgatgctc cgcgcacctc acagactccg cccgacgagg
     1621 aagccgagct tccatctgcg actcctgagc tcgtcggcga agcagcgccg cgcgaggtcc
     1681 ctcccgtgca cgcggcgagc gactccgacg ccggagtagt cgacgccgat gctccggcgt
     1741 tcgagcccgt ctcgctcgcg cctccactca cggatctgcc gactcgaccc gacgcgacgg
     1801 ctcttgccgc cccggtacat cctcccgccg agcgcgaacc ggaaccgtcc cccgtgcaga
     1861 cgtatccgga gccagcaccc gagccccccg cgcccgaggc tactcccgaa cccgcgatgg
     1921 agtctcccgc acccgtgtcc gacgccattc cgactcccgc gagcccagag ctccgtgtgg
     1981 cgccagccga gcccgagcct gcgtctggag tcgaagtgcc tgccgagcct ccaggtccgg
     2041 atgagttcgc agccgagata caggcggcgc ccgagccggc tcggttcggt ttggagcctg
     2101 tcgagccgag cgcgagcctc gagccagtgc aggaactcgc gccggagccg tcacagccgc
     2161 agagcgacat cgttccgacg ttgttgccgg cgcccatgcc cgagccggag cccgcgccgg
     2221 cgctcatcgt gctgcgccgg tcttcgcagg atccgttggt ttgttgggat cttcccgaaa
     2281 gccagctcgc cgcacattcg ccgctcgaac tcgactgcca cggcttttca gcgagcccga
     2341 aaggcactga gcgacgcagc gtcaccgtgt tggtcgagag ccgcaggggc agcgccgtgc
     2401 tctctggctt cgcgcgcgcg acgctgattc gagtggcgct cggtacgcgc tctgaaggct
     2461 ccttcttgcc gctcgtgatc gcttcggagc ttcgccaaag cgacgtgtcg ctcgacgtga
     2521 gctttcggcc tccgttgagc gagtcgaccc cgcccacccc catcgagcgc gggttgatcg
     2581 accaattcgc gaattgagtc gtcggacctc gccgttcact cgcgagcaga ttcgacgcaa
     2641 cccgttccgc ggttgaccga ccgtcgctca tgtggcgccg gcttctcatc ctgtccttcg
     2701 caatgtcgct cactcccaag gccctggcgg ggccgctgcg gctcagcgcc ctcttcgagt
     2761 gggagcgagg ggtccaacgg cagggctacg caggctttct gctgctagaa ataccgctcg
     2821 agcgcctggc
//
LOCUS       DASTKS010000739         6763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1010375, whole genome shotgun sequence.
ACCESSION   DASTKS010000739 DASTKS010000000
VERSION     DASTKS010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010375"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFQ35_15325"
     CDS             <1..1410
                     /locus_tag="VFQ35_15325"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HET9932073.1"
                     /translation="LIPLRNPRTQSPIASPVRLTPNRNQPLWVDVDVPRSAAAGNYRG
                     ALRVQPSPGAEVSIPIELEVFPFEIPARRGLTAWVPMYATRLIRAEGLPEEPPPEPYG
                     KLSHAYHEMAHAHRFWTQVIEEQPRVRWNEKEGTIQDADWKTYDAVNGPVLDGSLFED
                     HEPPPIWKVGGFIWWGARPGDPPHFGGNYQQDSALTPAHKRAMKEYATAIRAHFTEHG
                     WNHPRLFEYMIDEPDVDNHAGFVSLIADYGHALHAANANIDHLLTLAPRKELFDAVDI
                     WAVWAGGYVPSRMQERQRAGQKTWFYQHHEPFVGGNCLNNDGVSMRSWPWIAWRYRVD
                     AIFLWVGNFWNDDPYRKAINWDDELIGNGVFFYPGHLLTTLGYPALDGPVSSFRMKAL
                     RRGLFDYDYFELLRKAGGDPDKLVNRIVHSALNEEGWEPVWRHPRFEASGDWEHDGPR
                     WDAVRLEAAREIIARQPKQ"
     gene            complement(1468..2211)
                     /locus_tag="VFQ35_15330"
     CDS             complement(1468..2211)
                     /locus_tag="VFQ35_15330"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET9932074.1"
                     /translation="MSTREQNGQRDIQLAHAGTHAVVAGGTRHLSDRPGPDQVSAELL
                     EGLANTTEQLDAFRELRTQLMAMAARMDMSHFTTMVAPVSAASGGGFVARNLAASFTL
                     EESRVAVLIDCNLRDPTQHRAFGVDSEEGGLFDYLDKRQGAVTLRPTALPRLHLIPAG
                     RPDTRPREYFSGRTMRDIMAAFRQSSCFVVLDAPAVKGAPDARILSELADLVVLVAGY
                     GVDTAESVSEAAELFDKKKFAGVVFNEAR"
     gene            complement(2208..3755)
                     /locus_tag="VFQ35_15335"
     CDS             complement(2208..3755)
                     /locus_tag="VFQ35_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932075.1"
                     /translation="MSTTPEPLTETLALILKEGRRRLLALTGAFAFIAIVALPIGLNL
                     PKRWDASTLILAEHSNIIQPLMQGAAVPTDVTDQVAILNQIMARHRIMREVATFGGWL
                     DGKQSRADEERVINKVKARIKVDSPRPEMVRISYYDTDPVKAAKVANKLAEIYVREGT
                     AAKERESKEAFDFIDKRVTEYAIKLTEAHQAVIARYREQNKPAAAPVASAAGSAAAAR
                     PQVVAGAPRPAAPAPAPVRRPPPVSPEARRIEEQLSTRVGQLQNELSRLLSNYTEQHP
                     DVVRVRRDLAAAQAELRSAVDARMATERAAVEAAAGEDEVILAARAAVQRSGGPVLPR
                     AATGVPGTPGAVPGAYDPLNPMAAQDPTLRVVNQDVTLGELLNRYEATRAVYQDLLKR
                     RENARVSMDLDAEHRGLSLRVQEPAEVPATASSMRLLHLCLIGLVTAIAVPCGLLIAL
                     VKMDGRLRTERQIERFTKLPVLTTIPRAETPKTVSKDRFRTVLAAAMVAGVLAVYLVT
                     MIVRVRS"
     gene            complement(3919..5097)
                     /locus_tag="VFQ35_15340"
     CDS             complement(3919..5097)
                     /locus_tag="VFQ35_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9932076.1"
                     /translation="MQTSRELVPVPTGSLVVSPSTNASGPIALSLVVPTYNESANIRA
                     LLSRLVAALEPRLPGAYELIVVDDDSPDRTWEIALEIAASQPQVRVMRRTGERGLASA
                     VIRGWQVGQGNALAVIDGDLQHPPEVTVRLYDRIAEGFDIAVASRNAEGGGVSSWSMA
                     RRIVSRGAQLIGLIIVPSIVGRVSDPMSGYFIVTRAAIAGVPLSPLGYKILIEVMGRG
                     RIGKVAEEGYVFRERQEGSSKVTARLYLEYLRHLVRLRLTTESSLRFFRFAIVGLSGV
                     GVDMALLYLLSDPSQLGLGLTRSKFIAASAAIVNNFFWNERWTFRRAAQGKSGAIWRR
                     FVKFALVCGMGLALNVAVLNVLFNWGHVNRYVANLIAIFLVTVWNYWLNAKLTWNVRN
                     "
     gene            complement(5109..6611)
                     /locus_tag="VFQ35_15345"
     CDS             complement(5109..6611)
                     /locus_tag="VFQ35_15345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9932077.1"
                     /translation="MDDTATSAPSASWAGEELRSTLKFLAMPACIAAFPVVLGVQLPS
                     IVLYGAAGIVGLILINRLMRDPEWLMAAAIIYLPLNMMFVVPIGPGLNATNILLILLL
                     FAYYRYGRERDEPKLAPFATLVRAYMIMTLLSVVTAVITMGPGFIAERTLDVKGWLDQ
                     FIIFFVFLNLIRDANMARRMAVYMMLGSVVVAALGFQEWLEKRDYSSMEKSRLLGPQL
                     QPNDFGAFVAYASAPFLALALNNWRRLKPLMVAVPYLFVSARLLIATYSRGAYLGMAL
                     AGIVASYVRSKTFLLAGILIGAAVVVTMPEVLPESVVARMSQTAAQPASDIGGEKLDN
                     SSQTRLILWKAALTMTAYSPVFGWGFRTFPHFKGDFTETQVHESDNHNMYLYLSSQMG
                     VPAVVLLLLIFWRLYSAGVFVFRRNTDPFTRVIGMSAAAMAAAAFLVNMFGSRMVDIC
                     VTVHIWIMLAIIANLRATTPATPAGEVSVDSRPRTWSDVRAEEERATGPA"
BASE COUNT         1244 a         2214 c         2191 g         1114 t
ORIGIN      
        1 ctcattcccc ttcgaaaccc gcgcacgcag agccccatcg cgagccccgt gcgtttgact
       61 ccgaatcgca accagccgct ctgggtcgac gtcgacgtcc cgcgctcggc cgcggcgggt
      121 aactatcgcg gcgcgctgcg cgtgcagccc tctcccggag ccgaggtctc gatcccgatc
      181 gagctcgagg tgttcccctt cgagatcccc gcgcgacgag gcttgacggc gtgggttcca
      241 atgtacgcga cgcgcctgat tcgcgccgaa gggttgcccg aggagccgcc gcccgagccg
      301 tacggaaagc tttcgcacgc ctaccacgag atggcgcacg cgcaccgctt ctggacgcaa
      361 gtgattgaag agcagccacg cgtgcgttgg aacgaaaaag aaggaacgat tcaagacgcg
      421 gactggaaga cttacgacgc ggtgaacggg cccgtgctcg acggaagtct gttcgaggac
      481 cacgagccac cgccgatctg gaaggtgggc ggcttcattt ggtggggcgc tcgccccggt
      541 gatcctccgc atttcggcgg caattaccag caggacagcg cgctcacgcc cgctcataaa
      601 cgagcgatga aagagtacgc gaccgccatc cgcgcccatt tcaccgagca cggctggaac
      661 catccgcgcc tcttcgagta catgatcgac gagcccgacg tggacaacca tgccgggttc
      721 gtgagcctca tcgccgacta cggacacgcg ctccacgccg cgaacgcgaa catcgatcac
      781 ttgctgacgc tcgcgccgcg caaggagctc ttcgacgcgg tcgacatctg ggcggtgtgg
      841 gctggcggct acgtcccttc gcgcatgcaa gagcggcagc gcgcgggaca gaaaacttgg
      901 ttctatcagc accacgagcc gttcgtcggc ggcaactgct tgaacaacga cggcgtctcg
      961 atgcggagct ggccgtggat cgcgtggcgc taccgcgtcg acgcgatctt cctctgggta
     1021 ggcaacttct ggaacgacga cccgtatcgg aaggctatca attgggacga cgagctcatc
     1081 ggcaacggcg tcttcttcta cccgggccac ctcctcacga cgctcggcta cccggcgctc
     1141 gacggacccg tctcttcgtt tcgcatgaag gcgctgcgcc ggggcctctt cgactacgat
     1201 tacttcgagc tcctgcgcaa ggctggcggc gaccccgaca agctcgtcaa ccgcatcgtg
     1261 cactcggcgt tgaacgagga aggctgggag cccgtttggc gtcaccctcg cttcgaagcg
     1321 tcgggtgact gggaacacga cggtccacgt tgggacgcgg ttcgcctcga agcggctcgc
     1381 gaaatcatcg cgcggcagcc caagcaatag gcaaaggctg cgcgcaagcg cgcgttgccc
     1441 cgaggctccc gaaaagagct cgagctctca gcgcgcttcg ttgaagacga cgccggcgaa
     1501 ctttttcttg tcgaacagct cggccgcttc ggagacgctc tcggccgtgt ccacgccgta
     1561 gccggccacg agcaccacca aatccgcgag ctccgagagg atgcgagcgt ccggtgcgcc
     1621 tttgaccgcc ggcgcgtcca gcacgacgaa gcacgacgac tgccgaaacg ccgccatgat
     1681 gtcgcgcatc gtgcgccccg aaaagtattc gcgagggcga gtgtcgggcc ttcccgccgg
     1741 gatcaaatgc agccgcggga gcgccgtggg acggagcgtt accgcaccct gtcgcttgtc
     1801 caggtagtcg aagagcccgc cttcttcgct gtcgacgccg aaggcgcggt gctgggtcgg
     1861 gtcgcgcagg ttgcagtcga tgaggacggc gacgcggctc tcctcgagcg taaacgacgc
     1921 cgccaagttc cgcgcgacga agcctccgcc cgacgcggcg ctgacgggcg ccaccatcgt
     1981 ggtgaagtgg ctcatgtcca tgcgcgcagc catcgccatc agctgagtgc gaagctcgcg
     2041 gaacgcgtcg agttgttcgg tcgtgttcgc gagcccctcg aggagctccg cgctgacttg
     2101 atccggaccg ggccgatcgg agagatggcg cgtgcccccc gcgacgacgg catgcgtccc
     2161 cgcgtgagcg agctgaatgt ctctctgccc gttttgttcg cgggtgctca cgacctcacc
     2221 ctgacgatca tggtgacgag gtagacggcc agaacaccgg cgaccatcgc ggcggcgagc
     2281 acggtgcgga accgatcttt cgacactgtc ttcggcgttt cggcgcgtgg gatggtggtg
     2341 aggacgggga gcttggtaaa gcgctcgatt tggcgctcgg tgcgaagccg accatccatc
     2401 ttcaccaggg cgatcagcaa accgcaggga accgcgatcg ccgtgacgag gccgatgaga
     2461 cagaggtgaa gcaagcgcat gctgctcgcg gtcgccggca cctccgccgg ctcctggaca
     2521 cgcagcgaga ggccgcgatg ctcggcgtcg agatccatcg acacgcgcgc gttctcacgg
     2581 cgcttcagca gatcttggta cacggcgcga gtcgcctcgt agcgattcaa gagctcgccc
     2641 agcgtgacgt cttgattcac gacgcgcaga gtcgggtcct gagccgccat ggggttcagc
     2701 ggatcgtacg caccgggaac cgcgccgggc gtaccgggga cccccgtcgc cgcgcgagga
     2761 agcactggac caccgctgcg ctgcaccgcg gcgcgagccg ccagaatgac ttcgtcttcg
     2821 ccagcagccg cctcgaccgc ggcgcgctcc gtagccatgc gcgcgtccac ggcgctgcgg
     2881 agctcggcct gcgcggccgc caaatcgcgg cggacgcgga cgacgtcggg gtgctgctcc
     2941 gtatagttgg agagcaagcg gctgagctcg ttctgcagtt gaccgacgcg cgtgctgagc
     3001 tgctcctcga tgcggcgcgc ctcgggcgag accggcggcg ggcggcgaac cggggccggc
     3061 gcgggcgcag cggggcgcgg cgcgcccgca acgacctgcg ggcgagcggc ggcggcacta
     3121 cccgcggccg acgcgactgg cgcggctgcc ggcttgttct gctcgcggta ccgcgcgatg
     3181 acggcctgat gcgcctcggt gagcttgatg gcgtactcgg tgacgcgctt gtcgatgaag
     3241 tcgaaggctt ccttgctttc gcgctctttg gcggcggtgc cttcgcgcac gtagatctcc
     3301 gcgagcttgt tggcgacctt ggccgccttg accggatccg tatcgtagta ggagatgcgg
     3361 accatctcgg gtcgcgggct atcgaccttg atgcgcgcct tcaccttgtt gatgacgcgc
     3421 tcctcgtcgg cgcgactctg cttgccgtcg agccagccac cgaacgtcgc gacctcgcgc
     3481 atgatgcgat gacgcgccat gatctggttc aagatcgcga cttgatccgt cacgtcggtc
     3541 ggcacggccg cgccctgcat gagcggctga atgatgttgc tgtgctccgc gaggatcagc
     3601 gtcgaagcat cccacctttt gggaaggttc aagccgatgg gcagcgcgac aatcgcgatg
     3661 aacgcgaacg cgcccgtcaa cgccaacagc cgccgcctcc cctccttaag gattagcgcc
     3721 aacgtttcgg tcaacggctc tggtgtcgtg ctcatgaaca gaaccctgtc gcgaccaagg
     3781 taggccaata taacatgaac cgtcgcccga gcacgatttg ggccaaagca gtttccgctt
     3841 gaaatacgct gaaactcgag ctcctggctg gccgaacgag tccctccgtg cgcacgccag
     3901 tgcgccacgt ggcgtggctc agttgcggac gttccaggtc aatttggcgt tgagccagta
     3961 gttccagacc gtcacgagaa agatggctat caaattcgcc acgtagcgat tcacgtgccc
     4021 ccaattgaag agcacgttga gaacggccac gttgagcgcg agccccatcc cgcaaaccag
     4081 ggcgaactta acgaagcgcc gccagattgc ccctgacttt ccctgcgcgg cgcggcgaaa
     4141 agtccagcgc tcgttccaaa agaagttgtt cacgatcgcc gcgctcgcgg cgatgaactt
     4201 gctccgcgtg agcccgagtc ccaactgact cgggtcgctg agtaggtaga gaagcgccat
     4261 gtcgaccccg acgccgctca gacccactat cgcgaagcgg aagaagcgca ggctcgactc
     4321 ggtggtgaga cgaagtcgga cgaggtgacg caggtactcg aggtagaggc gcgcggtgac
     4381 cttgctcgag ccctcctgcc gctcgcgaaa gacgtaacct tcctctgcga ccttgccgat
     4441 gcggccacga cccatcacct cgatgaggat cttgtaaccg agggggctca gcggaacgcc
     4501 ggcgatggca gcgcgcgtca cgatgaagta gccgctcatc gggtcgctga cgcgtcccac
     4561 gatgctcggt acgatgatga ggccgatcag ctgtgcgccc cgcgacacga tgcgccgcgc
     4621 catgctccaa ctcgagacgc cgccgccctc ggcgttgcga ctggcgaccg cgatgtcgaa
     4681 gccctccgcg atgcgatcgt agagccgaac cgtgacctcg ggagggtgct ggagatcgcc
     4741 gtcgatgacc gcgagcgcat tgccctgccc tacttgccag ccgcggatga cggcgctcgc
     4801 gaggccccgc tctccggtgc gccgcatgac gcgcacctgc ggctgtgaag ccgcgatttc
     4861 gagcgcaatc tcccaggtgc gatcggggct gtcgtcgtcg acgacgatga gctcgtaagc
     4921 gccgggcagg cgaggttcga gcgcggcgac gagccggctc aagagagcgc ggatgttcgc
     4981 gctttcgttg taagtgggaa cgaccaacga gagcgcgatt ggtccgctcg cgttggtcga
     5041 aggggaaacc accaagctcc ccgtcggcac ggggaccagc tctctcgacg tttgcatgag
     5101 cgctgcgctc aagccggccc tgtcgctcgc tcttcctcgg cccgcacgtc cgaccacgta
     5161 cgcggacgtg agtcgaccga gacttcgccc gccggcgtcg ccggggtcgt ggcacgaaga
     5221 ttcgcgatga tggccaacat gatccagatg tgcacggtga cacaaatgtc caccatgcgc
     5281 gacccaaaca tgttgaccaa aaacgcggct gcggccatcg ccgccgcgct catccctatc
     5341 acccgcgtga acgggtccgt gttgcggcga aacacgaaga ctccagccga gtagagccgc
     5401 caaaagatga gcagcaagag cacgactgcc ggcaccccca tttgcgagct caggtagaga
     5461 tacatgttgt gattgtcgga ctcgtgaacc tgagtttcgg tgaagtcccc tttgaaatgg
     5521 gggaaggtgc gaaaccccca gccgaatacg gggctgtagg ccgtcatcgt gagcgcggcc
     5581 ttccacaaga tgaggcgtgt ctgagagcta ttgtcgagct tctctccgcc gatatcggat
     5641 gcgggctggg ccgccgtctg cgacatgcgc gcgaccaccg actcggggag cacctccggc
     5701 atcgtcacta cgaccgcggc gccgatcaaa atgccggcga gcaagaaggt cttgctgcgc
     5761 acgtagctcg cgacgatgcc ggcgagcgcc atgccgaggt aagcgccgcg cgagtaagtt
     5821 gcgatgagta ggcgcgcgga aacgaacagg tacggaaccg cgaccatgag cggcttcagg
     5881 cgacgccagt tgttcagcgc gagcgccaag aagggcgcgg aagcgtaggc cacgaacgca
     5941 ccgaagtcgt tcggttgcag ctgcgggccg agaagccgcg acttctccat cgagctgtag
     6001 tcgcgctttt cgagccactc ctggaagccg agggcggcga cgacgaccga gccgagcatc
     6061 atgtacacgg ccatgcggcg cgccatgttc gcgtcgcgaa tcaggttcaa aaatacgaag
     6121 aatatgatga actggtcgag ccaacccttc acgtcgagcg tgcgctcggc gatgaagcca
     6181 ggacccatcg tgatgacggc cgtcacgacc gacaacagcg tcatgatcat gtaagcgcgc
     6241 acgagggtcg cgaacggcgc aagcttcggc tcgtcgcgct cgcggccgta gcggtagtag
     6301 gcgaacaaca gcaagatgag gaggatgttc gtggcgttga gacccggccc gatgggcacc
     6361 acgaacatca tgttgagtgg caggtaaatg atcgccgccg ccatcaacca ctcggggtcg
     6421 cgcatcagac ggttgatcag gatgagcccg acgattcccg cggctccgta cagcacgatg
     6481 ctcggcagct gcacgcccaa aaccactgga aacgcggcga tgcacgctgg catggccagg
     6541 aacttcagcg tcgatcgcag ctcctcccct gcccagctcg cgctgggggc ggaggtggcg
     6601 gtgtcgtcca tcgcccgatc ctgcctcctc ggacccgctt gtccaccagc gattcaggag
     6661 ctcgctcgag ccctacccgt gcctcacgtc cgaccgccta gccggacctg agcgaccgag
     6721 cgccgcgcgt tgcagtgcaa cgaactgacc atgaagccac agt
//
LOCUS       DASTKS010000740         4733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_787270, whole genome shotgun sequence.
ACCESSION   DASTKS010000740 DASTKS010000000
VERSION     DASTKS010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787270"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFQ35_15350"
     CDS             <1..559
                     /locus_tag="VFQ35_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932078.1"
                     /translation="HEIPVMVVTVVDRAQKARALGADEFWLKPVDGQRLIRKLNELAA
                     RTPGSVRILIIDDDDAARYLIRKLLEQTTYVILEAKSGPEGVELARRERPHVIFLDFV
                     LGDITAFDVLDELKVNPTTRGIPVIIQTAKTLDESERERLRRETAAILSKQSLNREVA
                     IGRVREALLKAGSGATVIHDGVRDA"
     gene            552..2150
                     /locus_tag="VFQ35_15355"
     CDS             552..2150
                     /locus_tag="VFQ35_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932079.1"
                     /translation="MPEERSWRVLNVNDTESARYLVSRVLTSRGFEVTEARSGSEALE
                     LALKVQPDVIVLDIKLPDVSGYEVCRQLKNDPLTQSIAVIQTSATFVTSENKVLGLES
                     GADAYLTQPFEPIELVAMVSALLRLKRFEAEARRRADELAEANRRKDEFLAMLAHELR
                     NPLSTIVTGVKLLDEFNKESEQARNVCDAVQRQAKHLSRLIDDLLDVSRITQGKIELR
                     REPIELGALVRAAVEQQRPKFEKRDYRVELSLPNQPVYVHADATRVEQILVNLLNNAE
                     RYGERRGVIQVSLGVSETKEPRALLSVKDDGVGIAPEHLSSLFELFYQVDRSLARSRS
                     GLGIGLTMVKKLVELHGGQVQVRSDGLGHGSEFLVELPLSEPALAIEQPHAASTPEVG
                     DAGNLRILLVDDNVDSCDMFRGALELAGHRVDCAYDGADGLRMALEKSYDTAVIDIGL
                     PGLNGYEVAERLGHALGERRPFLVAMTGYGRQQDRKQSLEAGFDMHLVKPVAPNELER
                     VLAQSRRSPRRAASAPQSDGADLA"
     gene            complement(2387..3670)
                     /gene="nrfD"
                     /locus_tag="VFQ35_15360"
     CDS             complement(2387..3670)
                     /gene="nrfD"
                     /locus_tag="VFQ35_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9932080.1"
                     /translation="MVDSVQPTPFGSHTRNYPRFAWHVLRHSFQGSPRFYVWMTVLTA
                     IMLVGANAWAHQVARGMALTGMSDHVSWGLYIANFTFGVGLAAGAVLLVIPAYLYDDH
                     DMHDVVIIGEIVAIAAIVVCLLFITVDLGRPDRFWHIIPGVGRFNWPISMLTWDVIVL
                     NGYLLLNLHITGYLVYMRYIGRRPAKRWYLPFVFLSIGWAISIHTVTAFLYSGLGGRP
                     FWNSALLAPRFIASAFVSGPALVILLLQIVRRLTSFEIGDGPVHTLTRIMRITVLVNL
                     FMAASEFFTALYTGGAHGASARYLLVGMHGHAALVPWIWSAIALNIGSVVLIEVERRT
                     QAMRYLNLACVAAFAGVWIEKGMGLIVPGFIPSTLHELVEYAPSVTEWKVTLGIWAAG
                     GMVLTVALKVALPLMSSGRLAQPSAPPEPALLDSE"
     gene            complement(3678..4580)
                     /locus_tag="VFQ35_15365"
     CDS             complement(3678..4580)
                     /locus_tag="VFQ35_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9932081.1"
                     /translation="MVDSKRRVALKVLGGTLGAAAFARALAPLQSLAASTNLDEFLQR
                     HYKELTPEALRSVIVRLEAAALREYGKAVKIRDLRPQDGVGFAYALNLSVCIGCRRCA
                     EACHQENNHDRATHNSYIRVFEMQQGSIDFEKGDATYDHPVPQPGKYYLPVQCQQCDN
                     PPCVKVCPVQATWKERDGIVVVDYNWCIGCRYCEAACPYHARRFNWQKPTIAPDEINP
                     NQGYLSNRVRPQGVVEKCHFCLHRTREGRLPACLEACPTGARVFGNLLDPDSEIRWVL
                     ANKRVFVLKEELGTEPRFFYFFDK"
BASE COUNT          915 a         1449 c         1550 g          819 t
ORIGIN      
        1 ccacgaaata ccggtgatgg tcgtcacggt ggtagatcga gcgcaaaagg ctcgcgccct
       61 cggcgccgac gaattctggc tcaagcccgt cgatggccag cgcctgattc gcaagttgaa
      121 cgagctcgcg gcgcgtacgc ctggttccgt tcgcatcttg atcatcgacg atgacgacgc
      181 ggctcgctac ttgattcgga agctcctgga gcagacgacc tacgtcatct tggaggcaaa
      241 gagcggtccc gaaggcgtcg agctcgcgcg ccgcgagcgt ccccacgtca tcttcctcga
      301 cttcgtgctg ggtgacatca ccgcgttcga cgtactcgac gagctcaagg tcaacccgac
      361 tacgcgcggc attcccgtca tcattcaaac cgcgaagacg ctcgacgaat ccgagcgtga
      421 acgcctgcgt cgcgagacgg ccgccatcct ttcgaagcag agcttgaacc gcgaggtcgc
      481 gatcggccgc gtgcgcgaag cgctattgaa ggcggggtcc ggcgcgaccg tgatccacga
      541 cggagtccgc gatgcctgaa gagcgcagtt ggcgggtcct caacgtcaac gacaccgaga
      601 gcgcacgcta tctggtgtca cgcgtgttga cgtcgcgcgg cttcgaggtg accgaggcac
      661 ggagcggcag cgaggcgctc gagcttgcgc tgaaggtgca gcccgacgtg atcgtgctcg
      721 acatcaagct ccccgacgtg agcggctacg aggtgtgtcg acagctgaag aacgatccgc
      781 tcacgcagtc gatcgcggtg attcagacgt cggcgacctt cgtgacgagc gagaacaagg
      841 tgctcgggct cgagtctggc gcggacgcgt atttgacgca gcctttcgag ccgatcgagc
      901 tcgtcgcgat ggtcagcgcc ctcttgcgcc tgaagaggtt cgaggcggag gcgcgtcgtc
      961 gcgccgatga actcgccgag gccaaccgca gaaaagacga gttcttggcg atgttggcgc
     1021 acgagctccg aaatccactc agcacgatcg tcacgggggt gaagctgctc gacgagttca
     1081 acaaagagag cgagcaggcg cgcaacgtgt gtgacgcggt gcagcggcaa gccaagcacc
     1141 tctcacgact gattgacgac ttgctcgatg tctcgcgcat cacgcagggg aaaatcgagc
     1201 tgcgccgcga gcccatcgag ctcggcgctc tggtcagggc ggccgtcgag cagcagcggc
     1261 cgaagttcga aaaacgggac taccgggtcg agctcagcct tcccaaccaa ccggtgtacg
     1321 tccacgccga cgcgactcgc gtcgagcaga ttctcgtcaa cttgttgaac aacgcggagc
     1381 gatacggaga gcgtcgcggt gtaatccagg tgagcctggg cgttagcgaa accaaggagc
     1441 cccgcgcgct tctcagcgtc aaagacgacg gagttggtat cgcaccggag cacctttcgt
     1501 cgctgttcga gctcttctac caagtcgatc gctcgttggc tcgctcgcgg agcggtctcg
     1561 gtatcggctt gacgatggtc aagaagctcg tggagctgca cggcggtcag gtgcaggtgc
     1621 ggagcgatgg tctcggacac ggctccgagt tcctcgtcga gctgcctctg agcgagccag
     1681 cgctcgccat cgagcaacct cacgcggcct cgacccccga agtcggagat gccggaaatc
     1741 tgcgcattct gctcgtcgac gacaacgtcg actcttgcga tatgtttcgc ggtgcgctcg
     1801 agctcgcggg tcaccgcgtc gactgcgctt acgacggcgc ggatggtctg cgcatggcac
     1861 tcgagaagag ctacgacacc gccgtcatcg acattggact gcctggcctg aacgggtacg
     1921 aagtcgcgga gcggctgggc catgctcttg gtgaacggcg cccattcctg gtcgcgatga
     1981 ctggctacgg tcgccagcag gaccgaaagc agagcctcga ggctggcttc gacatgcatc
     2041 tcgtgaagcc cgtcgcgccg aacgagctcg agcgggtgct cgcgcaatcg cggcgatcac
     2101 cgcgccgcgc cgcctcggcc ccgcaatcgg atggcgccga tctagcttag tttagagcca
     2161 atcctaaatc cctctcgtcg ctcggcggag atccgcttcg cgttccttcg ttggtcctcc
     2221 tcggcgtact tcagtctcag gattcgtcac atgtcgcgag caggtaggcc ttcgtctcgt
     2281 tcgctcgcag agggctcgac acgaaagagt tggttccgac ccgggcgcca gccgagcgcg
     2341 agttggcccg atttctccgc gcctccgtgc gacgggagac gtcttgctat tcagagtcca
     2401 gcaacgccgg ctccggaggg gccgatggct gcgccaggcg tcctgacgac atgagcggca
     2461 gcgcgacctt cagcgcgacc gtgagtacca tgccgccggc ggcccagatg ccgagagtta
     2521 ccttccactc ggttacgctc ggtgcgtact cgactagctc gtgaagcgtg ctcgggatga
     2581 agccgggcac gatgagcccc atacccttct cgatccagac gccggcgaac gcggcgacac
     2641 acgcgaggtt gaggtagcgc atcgcttggg tgcgacgctc gacctcgatg aggacgaccg
     2701 agcctatgtt caacgcgatc gcagaccaaa tccatgggac gagcgcggcg tggccgtgca
     2761 tgccgaccag caggtagcgt gcggaggcgc cgtgagcgcc gcccgtatac agcgcggtga
     2821 agaactcgga cgcggccatg aagaggttga ccagcaccgt gatgcgcatg atgcgagtca
     2881 acgtgtgcac cgggccgtcg ccgatctcga agctggtgag gcgccgcacg atttggagca
     2941 gcaagatgac gagcgccggg ccactcacga acgcggaggc gatgaacctc ggggcaagca
     3001 acgccgagtt ccagaatggg cgccccccca accccgaata caagaaggcg gtgacggtat
     3061 ggatggagat tgcccatccg atcgacaaga agacgaacgg caggtaccag cgctttgccg
     3121 ggcgacgccc gatgtaccgc atgtatacga ggtacccggt gatgtgcaaa ttgagcaaca
     3181 agtagccgtt cagcacgatc acgtcccacg tgagcatcga gattggccaa ttgaagcggc
     3241 cgacgcccgg gatgatgtgc cagaagcgat cggggcgacc gagatcgacg gtgatgaaca
     3301 gcaaacaaac gacgatggcc gcgatcgcga cgatttcgcc gatgatgacg acgtcgtgca
     3361 tgtcgtggtc gtcgtataga tacgcgggga tgacgagcag cacggcgccc gcggccaacc
     3421 cgacgccgaa cgtgaagttc gcgatgtaga ggccccacga gacatggtcg gacatcccgg
     3481 tgagcgccat cccccgcgcg acctggtgcg cccaggcgtt ggcacccacc aacatgatgg
     3541 cggtcaacac cgtcatccaa acgtagaagc gtggcgagcc ttggaagctg tgccgaagca
     3601 cgtgccacgc gaagcgggga tagttccggg tgtggctacc gaacggagta ggctgcaccg
     3661 agtcgaccat ggtcttgcta cttgtcgaag aagtagaaga agcgaggctc ggttccgagc
     3721 tcctccttca gaacgaacac tcgtttgttc gcgagcaccc agcgaatttc gctgtccgga
     3781 tcgaggaggt tgccaaacac gcgcgcgccg gtcggacagg cttcgaggca agcgggaagg
     3841 cgcccttctc gcgtgcgatg caggcaaaaa tggcactttt cgacgacgcc ctgagggcgg
     3901 acgcgattgg aaaggtagcc ctggttcggg ttgatctcgt cgggggcgat cgtcggcttc
     3961 tgccagttga agcgccgcgc atgatacggg caggccgctt cacagtatcg acagccgatg
     4021 caccagttgt agtcgacgac gacgataccg tctcgctctt tccaggtggc ttgcaccggg
     4081 caaaccttga cgcagggagg gttgtcgcac tgctgacatt ggaccggcag gtagtacttg
     4141 ccgggctgag ggacggggtg atcgtaggtg gcgtcgccct tttcgaagtc gatgctcccc
     4201 tgctgcattt cgaagacacg gatgtaggag ttgtgggtcg cgcgatcgtg attgttctct
     4261 tggtggcaag cttccgcgca gcggcgacag ccgatgcata ccgagaggtt cagcgcatag
     4321 gcgaagccca cgccgtcttg cggtcgtaaa tcgcggatct tcaccgcctt gccgtactcg
     4381 cggagcgcag ccgcttcgag ccgaacgatg acggagcgaa gcgcctccgg cgtcagctct
     4441 ttgtagtggc gttgcaggaa ctcatccaag ttcgtgctcg cggcgaggct ctggagaggc
     4501 gccagcgctc gcgcgaacgc tgcggcgccg agcgtgcccc cgagcacctt cagcgcgaca
     4561 cgccgtttac tatcgaccat gggtttcctc tcgatgacgg gccacgaacc gatcgcgcgg
     4621 ggcgggcgcc ggaacgacct gcgggatctt cggcgtgtgc ggatcgtggc agtccacgca
     4681 gttgttgcgc gagcgaggac cactcgcgag atcccaggag ccgatcatcc cgc
//
LOCUS       DASTKS010000741         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_787272, whole genome shotgun sequence.
ACCESSION   DASTKS010000741 DASTKS010000000
VERSION     DASTKS010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787272"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..339)
                     /locus_tag="VFQ35_15370"
     CDS             complement(25..339)
                     /locus_tag="VFQ35_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932082.1"
                     /translation="MNGDIETSRVKFSTKVALAIGFALLVAVGALWLRDEPERPSLRS
                     VDDLLIGGAPATSRRLRVGGTVVAGSFKRGAGGCGLELRLRGSERRDGARTPAHCGCP
                     IC"
     gene            complement(336..1559)
                     /locus_tag="VFQ35_15375"
     CDS             complement(336..1559)
                     /locus_tag="VFQ35_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET9932083.1"
                     /translation="MSRYTVVVTPWGHPKVSRTLSLSGRHSLEQLHAAIIGAFGFADD
                     HLHAFFLNGHAWDREFGYFSRPDIGQKDTAGTKLDSLALVQNKSFLYLFDYGDEHRFG
                     VRVTEIEAQSEGPKQPELLASAGVAPKQYEIGESEDELDDAAELEGAGATAKATPLPE
                     PDPAWRDLVTRLGACLPHEVENEVANETEPSRPGSAEQLALATELLDRVGTDEAMLDR
                     VGEWTGGIVDWLIDLASALSREDKPAEAWALSQRLHAFSFREVLEPSMARWAALSGER
                     ERALELIATSLRNHPEDAAVLLELGAAWEELGDAAQAERCYREGLRFAGTDLAFRKEA
                     FGALLRLLKEQGRSIEARQAEHSEARWRDRFLSTAALNGVQRTVQNSASKVGRNDPCP
                     CGTGKKYKKCCGAAG"
     gene            complement(1649..>2499)
                     /locus_tag="VFQ35_15380"
     CDS             complement(1649..>2499)
                     /locus_tag="VFQ35_15380"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9932084.1"
                     /translation="CKRPVCGDGILSAGETCEDGNRKDGDGCSSACQTEVCGDNKKTG
                     IEECDDGNRNDNDGCSATCRTEVCGDGIVQRPREECEDRNTVDTDLCRNGCKNAASLN
                     ALSNSCANTDQITQTVCMVAVANWCKQFDNSPLAGMVTGQKGDNEYSVGCINGFKREE
                     VPTSSLNDKCGGGRQQSPACLDQAAAACQRKGYTQGFYLGAGSAADSTALACGMGAKR
                     GPESVQACNGIADTSPVPVDCAKALADKCGSGKGGMIQALAQKTQVTYTCIDLNLTGS
                     ARLKAP"
BASE COUNT          406 a          838 c          772 g          483 t
ORIGIN      
        1 ccctggcgcc gcctccacgt ttcgtcagca aatcggacag ccgcagtgcg cgggcgtgcg
       61 agcaccgtcg cggcgctcgg aaccacgaag ccgaagctcc aggccacaac ctccggcgcc
      121 gcgcttgaaa cttcccgcga ctacggtgcc tccgacgcgc aagcgtcgag acgtcgctgg
      181 tgctccacca atcagcaaat cgtcgacgga tcgcagggaa gggcgttcgg gctcgtctcg
      241 aagccacaac gcacccacgg cgacgagcag cgcgaaacca atcgcgagcg cgaccttggt
      301 cgagaacttt acgcgagatg tttcgatgtc gccgttcaac ccgcggctcc gcagcacttc
      361 ttgtattttt tgccagtgcc gcaggggcaa ggatcgttgc ggccgacctt ggacgcgctg
      421 ttctggaccg ttcgctgaac gccgttcagc gctgcagtcg agagaaaacg gtcacgccag
      481 cgcgcttcgc tgtgctctgc ctgtcgcgct tcgatcgaac gcccctgctc tttcagcaga
      541 cggagcagcg ccccaaacgc ctctttacga aaggccaggt cggttccggc gaagcgcaga
      601 ccctcgcggt agcaccgctc cgcctgtgcg gcgtcgccca gttcttccca ggcggcgccg
      661 agctcgagga gaactgcggc atcttcagga tggttacgaa gactggtcgc gatgagctcg
      721 agcgcgcgct cccgctctcc cgataaggcg gcccagcgtg ccatcgacgg ctcgagcacc
      781 tctctaaacg agaacgcatg aagacgctgg gaaagtgccc aggcttcggc cggcttgtcc
      841 tctctcgata gcgcggaagc aaggtcgatc agccaatcga cgatgccgcc ggtccactct
      901 ccgactcgat cgagcatggc ttcgtcggtg ccgacgcggt cgagcaactc ggtcgccaga
      961 gcgagctgct ccgcgctgcc tggtcgcgac ggctcggtct catttgcgac ctcgttctcg
     1021 acctcgtgcg gaaggcaagc gccgagtcgg gtgaccaagt cgcgccaagc gggatccggc
     1081 tccggtagtg gcgttgcctt cgccgttgcg cccgcgcctt cgagctcagc ggcatcgtcg
     1141 agctcgtctt cgctctcgcc gatttcgtac tgcttcggcg cgacgcccgc ggacgcgagg
     1201 agctcgggtt gcttcggccc ttcactttgc gcctcgatct ccgtgacgcg aacgccgaag
     1261 cggtgttcgt caccgtagtc gaagaggtac aagaagctct tgttctgcac cagtgcgagc
     1321 gagtcgagct tcgtgccggc cgtgtctttt tggccgatat ccgggcggct gaagtaccca
     1381 aactcacgat cccaagcgtg cccgttgagg aagaaggcgt gcaggtggtc gtcggcgaac
     1441 ccgaatgcac cgatgatcgc cgcgtgcagc tgctcgagtg aatggcgccc cgacagcgag
     1501 agcgttctcg agactttggg atggccccac ggtgtaacga cgacggtata gcggctcacg
     1561 cccggaaccg aatccggttc gagtcggagc gcaacccaaa tcgaaacgtg cgctgattaa
     1621 ggccggcccg agcgaatggc ggccgaattc aaggcgcctt caaccgagca ctgccggtca
     1681 ggttcaagtc gatgcacgtg tacgtgactt gcgtcttctg ggcgagagcc tgaatcatgc
     1741 cgcctttgcc gctaccgcac ttgtccgcga gcgccttggc gcaatcgacg ggcaccggcg
     1801 acgtgtccgc gatgccattg caggcttgca cgctctcggg tccccgcttc gcgcccatcc
     1861 cgcacgcgag cgccgtcgag tccgcggccg agccggcgcc gagatagaac ccctgcgtgt
     1921 accccttgcg ttggcaagcg gcggcagctt ggtcgaggca cgcggggctt tgttgacggc
     1981 cgcctccgca cttgtcgttg agtgaagagg tgggcacttc ttctcgcttg aagccgttga
     2041 tgcagccgac gctgtactcg ttgtccccct tctgcccggt gaccatgccc gccaaggggc
     2101 tgttgtcgaa ctgcttgcac cagttggcga ccgcgaccat gcaaacggtc tgcgtgatct
     2161 gatccgtgtt ggcgcacgag ttgctcagcg cattcagcga agccgcgttt ttgcagccgt
     2221 ttctgcacag atcagtgtcc accgtgttcc ggtcttcgca ttcctcgcgc ggccgctgaa
     2281 cgatcccgtc gccgcacacc tcggtgcggc aggtcgcgct gcagccgtcg ttgtcgttcc
     2341 ggttgccatc atcgcactct tcgatgcccg tcttcttgtt gtcgccgcaa acttcggtct
     2401 ggcacgcgga gctgcagccg tcgccgtcct tccgatttcc gtcttcgcag gtttcccccg
     2461 ctgacaagat cccgtctccg cagacgggcc gcttgcagg
//
LOCUS       DASTKS010000742         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_118933, whole genome shotgun sequence.
ACCESSION   DASTKS010000742 DASTKS010000000
VERSION     DASTKS010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118933"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2034)
                     /locus_tag="VFQ35_15385"
     CDS             complement(<1..2034)
                     /locus_tag="VFQ35_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932085.1"
                     /translation="MHTPSQRELRLFGVVARQAADWIERHLAEGALRASEEKYRALFE
                     NVDEGVARIELLFDERGQPNDFRWLDYNPAFTRHTGLANVLGKRGREAVPNFDADVLE
                     IYAGVARAGQGIRFERFAPKLERWFSIFVSPVLGDANQLVVIFANVTERKRAEEALRR
                     SEARNAYLVRLGDALRPLGDPGAVKRAAMQVLGEQLAVNRAFYGEFDGDDLLVEHAYE
                     SGVPPMPVGGPSKPSPAPWMRATLRAGENVIIADVRTDSRFASELRDGALSIHAIGAV
                     TVPMMKDGRLASSVSVHTDSPRTWAAEEVSLIEETAERTWEAVERARAEAALRESERA
                     ARTLLGEATRARADAEAANRAKDEFLATLSHELRTPLAAMLLWARSMRAGGVKPAEIS
                     RAVDAIVQSAESQSRLIDDLLDLSRLTAGRIELQRSAVDLRRVVEQAVDTVKPMAAEK
                     SVNLLVSVHREMGSVLLDEQRVKQVLWNLLSNAVKFTPKGGTVRVSATTVEGFIELEV
                     IDDGEGIDAAFLPHVFDKFRQADMADTRRHMGLGIGLTIAKRLTELHGGSIAATSAGL
                     GLGAKFRIRLPWVPAIAPPAPSRPGTLTPLDGLRTLLVEDDTSTLLAMKSSLENAGAR
                     VAPFEDGGEALAALERGLDVDVIVSDLGLPRMSGFELIARIGRHYTKDGRPIPP"
BASE COUNT          357 a          711 c          633 g          355 t
ORIGIN      
        1 aggtggaatt ggccgaccat ccttcgtgta gtggcgcccg attcgcgcga tcagctcgaa
       61 gccgctcatc cgcggcagac cgagatcgct gacgatgacg tccacgtcga gccctcgctc
      121 caaagccgcg agcgcctccc cgccgtcttc gaacggtgcc actctcgcac ccgcgttctc
      181 gagagaagac ttcatcgcga gcaacgtgct cgtgtcgtcc tcgacgagca gcgtgcgaag
      241 gccgtcgaga ggagtaagcg tccctgggcg cgatggcgcg ggcggcgcga tggccggtac
      301 ccacgggaga cggatgcgaa atttcgctcc aagtcccaag ccggcgctgg tcgctgcgat
      361 gctgccaccg tgaagttcgg tgagccgctt cgcgatcgtg aggcctatac cgagacccat
      421 gtggcgacga gtgtcggcca tgtccgcctg gcggaacttg tcgaagacgt ggggaagaaa
      481 agcggcgtcg ataccctcac cgtcgtcgat cacctcgagc tcgatgaatc cttcgaccgt
      541 cgtcgcgctg actctgaccg taccaccctt gggtgtgaac ttgacggcgt tcgatagcag
      601 gttccagagc acctgcttca cgcgctgctc gtcgagcagc accgacccca tctcgcgatg
      661 gacgctcacc aagaggttga cactcttttc ggccgccatc ggcttcaccg tgtccaccgc
      721 ttgctcgact acgcgccgaa ggtcgaccgc tgagcgctgc agctctatcc tgcccgcggt
      781 caggcgagac aggtccaaga ggtcgtcaat caaccgagac tggctctcgg cgctctgcac
      841 gatcgcgtcc accgctcgcg agatctccgc gggctttacg ccaccggcgc gcatggagcg
      901 tgcccaaaga agcatcgccg ccagcggggt gcgcagctcg tggctcagtg tcgcgagaaa
      961 ctcgtccttg gcccgattcg cggcttccgc gtccgcgcgc gcccgagtgg cctcccccag
     1021 cagcgttcgc gccgcccgct cgctctcccg caaagctgcc tccgcgcgcg ctcgctccac
     1081 cgcttcccac gtgcgctctg cggtctcttc gatcagcgaa acctcctcgg ctgcccacgt
     1141 gcgcggagag tcggtgtgaa cgctcacgct cgaggcgagg cggccgtcct tcatcatcgg
     1201 gacggttacc gctccgattg cgtggatact cagagcgcca tctcgcagct ctgaagcaaa
     1261 gcgcgaatcg gtccgaacgt cggcgatgat gacgttctcc ccagcgcgaa gcgtcgcgcg
     1321 catccacggc gcgggagaag gcttcgaagg cccaccgact ggcatcgggg ggacgccact
     1381 ctcgtaggca tgctcgacca acaagtcatc cccgtcgaac tcgccgtaga acgctcgatt
     1441 gaccgcgagc tgctcaccga gcacctgcat cgcggcacgc ttcacggcgc caggatcacc
     1501 gagcggccgg agcgcatcgc cgagccggac caagtaggcg ttgcgcgcct cgctgcgcct
     1561 caacgcctcc tcggcgcgct tacgctcggt gacgttcgcg aagatgacta cgagctgatt
     1621 ggcgtcgcct agtaccggtg aaacgaatat cgaaaaccat cgctcgagct tgggagcaaa
     1681 gcgctcgaag cgaatacctt gtcccgcacg cgcaacgccg gcgtaaatct cgagcacgtc
     1741 cgcgtcgaag ttcggaacgg cctcgcgtcc tcgcttgccg agcacgttcg cgagaccggt
     1801 gtgtcgcgtg aaagcggggt tatagtcgag ccagcgaaag tcgttcggct ggccgcgctc
     1861 gtcgaacaag agttcgatgc gcgcgactcc ctcgtcgacg ttctcgaaca gggctcggta
     1921 cttttcctcg ctcgcgcgca gcgcgccctc tgcgaggtgc cgctcgatcc aatcggcggc
     1981 ctgccgcgcg acgacgccga acagccgcag ctcgcgctga gaaggcgtat gcacgtcgcg
     2041 ccaatgggtc gaaatc
//
LOCUS       DASTKS010000743         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_787360, whole genome shotgun sequence.
ACCESSION   DASTKS010000743 DASTKS010000000
VERSION     DASTKS010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787360"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFQ35_15390"
     CDS             complement(<1..314)
                     /locus_tag="VFQ35_15390"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9932086.1"
                     /translation="MRQARVMTYNVHGFVGTDGVYDPERIASAIEQNQPDLVALQEVE
                     FGASARDQEARSEWLGTRLGMHCHFTLTRPGSSGGQFGNAVLTRHAFELVSEGELPRL
                     GS"
     gene            471..1055
                     /locus_tag="VFQ35_15395"
     CDS             471..1055
                     /locus_tag="VFQ35_15395"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9932087.1"
                     /translation="MTTQMRAQFDGRHVTLLALRWLAGVLGLLVVMIGLASFFRAPLE
                     HVGRVFVERFGYVGMGLGTFIADGFHFPIPPQFYMLLSVTSGGSELAAFGAITLGSLA
                     GGLAGYKFSAHLAHVEWLAKRLEPSRAQAQHLFDRYGYVAAVMSTFLPIPYSVLCYLS
                     GLNGLPHRVFLVLSLCRIPRLVFFFLVIRLGWHF"
     gene            complement(1088..1939)
                     /locus_tag="VFQ35_15400"
     CDS             complement(1088..1939)
                     /locus_tag="VFQ35_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932088.1"
                     /translation="MLCAALSGAHRVRVIRGGMACGEALGGEVLLETRDTESVRELVA
                     ALRITDGSGGQCQLCLGDPTIELYVGDRRRVILGVHHGTHVRWSEWHDDARLVSARAL
                     LTWLEQRGISYPSRYGALDGATLESRARQRWLDAVPRCLRAFAENDLLRELSVAPARF
                     MEALLVAFPDPRDQALALFEWLGNGAGSWSAYPVYESAAERLLLCLPFTALAECLRSE
                     LSAAQLEGAARFFTGWQFQNERRADATRLPTMAAKRLLEHALRSHDPEKERLARKAFG
                     LLAYSQS"
     gene            complement(2259..2978)
                     /locus_tag="VFQ35_15405"
     CDS             complement(2259..2978)
                     /locus_tag="VFQ35_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET9932089.1"
                     /translation="MASANDAVSYACPNCGAPVAATARACTYCHVALATRRCTHCFDH
                     SAASARHCATCGRELGLEPVAEASELSCPACRNALAALTEAGGTLFDCARCGGHFVEH
                     ALFRALIEQRASLAQSSTIAPARSALQTQKVTYRPCARCGALMHRKNFGGTSGVIVDV
                     CPVHGIWFDAGELPEVLTFVERGGLERARQRDEERARRARIEERSAHSKPFTNLESLE
                     NGSTKESALVELITNFLLWLV"
     gene            complement(3176..4042)
                     /locus_tag="VFQ35_15410"
     CDS             complement(3176..4042)
                     /locus_tag="VFQ35_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932090.1"
                     /translation="MRFGLVCAVGLSVSASGCSLLSLSHPRNGAPPKEVRQACGGKND
                     AALYPLADGSWVVLGALDVYLASSKLETEGDPSTRSWLKAQRVFGYTSAILAGISMVH
                     GIAVAVDCASRERPRREMRPSAPEEAARPEPAATSFPWEVLGYQFGTSPAAAKSVCQG
                     SGGVWVDAAGENPAQCQKSGLGPAVRLTFELGSLTELTLVYRPVRERMNEVFFELEGS
                     LQKYYGKGSRSKRELAPACNQSLYACLLNGDRPPASTWSWPDGSIELVPGFDSETPAL
                     LIHYAHTPNVGP"
     gene            complement(4215..>4429)
                     /locus_tag="VFQ35_15415"
     CDS             complement(4215..>4429)
                     /locus_tag="VFQ35_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932091.1"
                     /translation="PVLGVAVGEEVRGIWGFAALYGFPWFGVGISAQFPIATERPYWL
                     EKVGVAFRFQLPLSPCRSEFFSCAKK"
BASE COUNT          762 a         1469 c         1393 g          805 t
ORIGIN      
        1 tcactgccga ggcgaggcag ttctccctcc gacaccaact cgaaagcgtg gcgcgtgagc
       61 acggcgttgc caaattgccc cccgctcgag ccgggccgag tgagcgtgaa gtggcaatgc
      121 attccgagcc gagtgccgag ccactcgctg cgggcctctt ggtcccgcgc gctcgcgccg
      181 aactcgacct cctgcagcgc caccaaatcg ggctgatttt gctcgatcgc gctcgcgatg
      241 cgctcggggt cgtagacacc gtcggtgccg acgaacccgt gcacgttgta tgtcatcacg
      301 cgtgcctgcc gcatgaggct gattggagtt cctttgctga tgatccggat gcgtgagcac
      361 ttgcacccac cgttccaatc tcgcaggtcc ggagggggag ctcgcggaca cgccccgcgc
      421 gcaagtttgc ctcagtcggc ggcgccggaa atcgatgtca cgttggtagt gtgaccactc
      481 agatgcgcgc ccagttcgac gggcgtcacg tcaccctcct agcgctgcga tggctggctg
      541 gggtgctcgg cttgctcgtg gtgatgattg ggttggcgag cttctttcgt gcgccgctcg
      601 agcacgtggg gcgcgtattc gtcgaacgct tcgggtacgt gggcatgggg ttgggtactt
      661 tcatcgccga cggctttcat ttcccgatcc ctccgcagtt ctatatgctt ctcagcgtaa
      721 cctcgggcgg ttccgagctc gctgcgttcg gcgccatcac gctcggctcg ctcgccggcg
      781 gtctcgccgg ttacaagttc tcggcccacc tcgcgcacgt cgagtggctc gcgaaacggc
      841 tcgagccttc gcgcgcgcaa gctcaacact tgttcgaccg ctacggttac gtcgccgcgg
      901 tcatgtcgac gttcctcccc atcccgtatt cggtgctctg ttacttgtcg ggactgaacg
      961 ggctgccgca ccgagtgttc ctcgttctct ctctgtgccg catcccgagg ctagtcttct
     1021 tctttctcgt gattcgcttg ggctggcact tttagcggcc aaacctgcgc cgcgatggat
     1081 tggggcttta ggattggctg taggcgagaa gcccgaaggc tttgcgcgcg agcctttctt
     1141 tttcaggatc gtgactgcgc agggcatgct cgagcaagcg tttggcagcc atcgttggga
     1201 ggcgagtcgc atcggcgcga cgctcgttct ggaactgcca cccggtgaag aaccgcgctg
     1261 ctccttcgag ttgagcggcg ctgagctcgg agcgcaggca ctcggccaaa gccgtgaatg
     1321 gcagacacaa caagagccgc tccgcggctg attcgtagac ggggtaagcc gaccaggagc
     1381 ccgcgccatt gccgagccat tcgaacaacg cgagcgcctg gtcgcgaggg tcggggaacg
     1441 ctacgagcag cgcctccatg aagcgcgccg gcgcgacgct gagctcgcgc aagaggtcgt
     1501 tttcggcgaa tgcccgcagg caccggggca cggcgtcgag ccagcgctga cgagctcgcg
     1561 attcgagcgt ggccccatcg agcgctccgt aacggcttgg atagctgatg ccccgctgct
     1621 cgagccaggt gagcaacgcg cgtgcggaaa cgaggcgagc gtcgtcgtgc cactcgctcc
     1681 agcgcacgtg agtcccatgg tgaacgccga gaatgactcg gcgccggtcg cccacgtaaa
     1741 gctcgatggt cgggtctccc aagcagagct ggcactggcc gccgcttccg tccgtaatgc
     1801 gcaacgcggc tacgagctcg cggacgctct ccgtatcgcg ggtttcgagc aacacttcac
     1861 cgccgagcgc ttcgccgcac gccatgccgc cgcgaatgac tcgtacacga tgggctccgc
     1921 tcaacgccgc gcacagcatg gcatcgctcg ggcttgggcc gtacgcagag atgtttccct
     1981 tccccctcac ggaaccattg cccgagcttg tcgcagagac gaccgccata tcgtgctcga
     2041 caccagcgga gagccccaaa ggccccgcat ccccttcagc gtaagctagc gggcggagtc
     2101 gcgcaacgag tgcgggcgac gcgcactact catttaagtc tggttcgaga gacgcgcccc
     2161 agctcggcgc ggcggggtcc gtcgctcggc ggcaaaaatc tgacggaggc tgccacgctt
     2221 tgcggcagta ccgcctcgag cggggtcggg gcctttgctc aaaccagcca gagcaggaaa
     2281 ttggtaatca gttcgacgag agcgctttct ttcgtgctcc cgttttcgag cgattcgaga
     2341 ttcgtgaacg gcttactatg tgcgctccgc tcttcgatgc gcgcgcgccg ggctcgttcc
     2401 tcgtctcgtt ggcgagccct ttcgagcccg ccgcgctcga cgaaggtcaa cacctccggc
     2461 agctcacctg catcgaacca gatgccgtga acggggcata cgtccacgat cacgccgctc
     2521 gtcccgccga aattcttccg atgcatcagg gccccgcagc gagcacacgg gcgataggtg
     2581 accttttgcg tctgcaacgc cgagcgcgcc ggggctatgg tcgagctttg ggcgaggctt
     2641 gcgcgctgct cgatgagcgc tcggaagagc gcgtgctcca cgaagtggcc gccgcagcgt
     2701 gcgcaatcga agagtgtgcc gccggcttcg gtcaatgctg cgagcgcatt ccgacacgct
     2761 gggcaagaga gctcggatgc ttccgcgacg ggctcgaggc cgagctcgcg tccgcaggta
     2821 gcgcagtgcc gtgccgacgc cgcgctatgg tcgaagcagt gcgtgcaccg ccgcgtcgcg
     2881 agcgcaacgt gacaataggt gcaggctcga gcggtcgcag cgacgggagc tccgcagttt
     2941 gggcacgcat agctgacggc gtcgtttgcc gaggccatgt cgcaatcgta gccatgtagc
     3001 cgcgcggagc taccggcctt gctgcgctgc gaggattgct gggctcggag aggggcaggg
     3061 atgaagcggg taccgatgcc gatacatgcc ggggcccagg ccgcacaagt gtgtgtgtgt
     3121 caggtgaacg gcgctggttc tggagcggcc gacttggccc tcgaagcgag gctagtcagg
     3181 gcccgacgtt tggcgtgtgc gcgtagtgga tgagcaacgc gggcgtctcg ctatcgaagc
     3241 ctggtacgag ttcgatgctg ccgtcaggcc agctccacgt cgacgcaggc ggccgatcgc
     3301 cattcaacaa gcaagcgtag agcgactgat tgcacgcggg agcgagttcg cgtttcgacc
     3361 gcgagccctt gccgtaatat ttttgcagcg acccttcgag ctcgaagaac acctcgttca
     3421 ttcgctctcg tacgggccgg tagacgagcg tcaattcggt gagtgagcca agctcgaacg
     3481 tgaggcggac agccggcccc agcccgctct tctgacattg cgcggggttc tctcccgcgg
     3541 catcgaccca gacgccgccg gaaccctggc acacgctctt cgcggctgcc gggctcgtcc
     3601 cgaactgata cccgagcacc tcccacggaa aagaagtcgc cgcgggctcc gggcgcgctg
     3661 cctcctccgg agcggagggg cgcatctctc gccgcggacg ttcacgcgag gcgcagtcca
     3721 cagccacggc gatgccgtga accatagaaa tgcccgccaa aatggcgctc gtgtacccga
     3781 acacccgctg cgccttcagc cacgagcgag tgctcgggtc accctccgtc tcgagtttcg
     3841 acgaggcgag atacacatcg agcgccccca gcaccaccca gctgccatcc gcgagcggat
     3901 agagcgccgc atcgttcttc ccgccgcaag cttgccgcac ttccttggga ggcgcgccgt
     3961 tgcgcggatg agacagcgac aacagcgagc acccgctcgc gctcaccgac aacccaaccg
     4021 cacaaaccaa tccaaatctc atggctttct catcggtcga ctcgcggcac cagttgctac
     4081 tcttttttcg acgtacccgg gagaggatcg ccgctgcccc ccgtcgacga acccattggt
     4141 ttcaaatcgt cggcgcggtc actcggagtc ggcacggtga cgtgtccatc gggcacgccc
     4201 cccgccctac ctaactactt cttcgcacag gaaaagaatt ccgagcgaca tgggctcaac
     4261 ggcagctgga accgaaacgc caccccgact ttctcgagcc aataagggcg ttccgtcgcg
     4321 attgggaact gcgcggagat gccaacccca aaccaaggga atccatacaa cgcagcgaac
     4381 ccccagatcc ctctcacctc ctcgccgacc gcaacaccga ggacgggct
//
LOCUS       DASTKS010000744         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_787466, whole genome shotgun sequence.
ACCESSION   DASTKS010000744 DASTKS010000000
VERSION     DASTKS010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787466"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFQ35_15420"
     CDS             <1..1227
                     /locus_tag="VFQ35_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932092.1"
                     /translation="LPSESKRERSPSWDSVSAAAKLRAEPVAPAATDLSASAQLPTPP
                     APRQPTPWFSRLVLIGGAMLVMGGSLAVAVRLFMTHDAQVEKPPAPPPEASAVAAPSE
                     APPDSPVASAESPAPPLLESAAAVPPAPPVSSAASAAPPAPPAAEPDAPKQAAALAGV
                     PIRDVTGGPVGRAVIGSYRLLAREAAAGRDFYGAAKECADVGMALCSEAQWMHACSAF
                     PELGAVASWTSTLKGEHVVVRGGQDCAAESKAAPVERSLNRTGMCCDRAIAMTTDNLQ
                     KNYLTTTGERVLAIERALNQRNMSSLLELSESSVAVDDQPKSPAQLRALVESAFKSAP
                     GIYVLNDTCHFSVQAKKITKGTRRKKKVVYQTQGWTAECDQTAIVPSTSVSARKVTYG
                     FSASSKLREITTKPEE"
     gene            complement(1235..1684)
                     /gene="dtd"
                     /locus_tag="VFQ35_15425"
     CDS             complement(1235..1684)
                     /gene="dtd"
                     /locus_tag="VFQ35_15425"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9932093.1"
                     /translation="MRAVVQRVSSADVRVDGVVVGSIERGVCVFVGVAREDDAASARE
                     LADKVVGLRIFEDEAGKMNLPLAAVGGKVLAVSQFTLLGDCRKGRRPSFAHAMAPETA
                     EQLFETFCSACRELGVAVETGRFRTEMRVSLVNEAPVTLLLDTTRLF"
     gene            1772..2260
                     /locus_tag="VFQ35_15430"
     CDS             1772..2260
                     /locus_tag="VFQ35_15430"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HET9932094.1"
                     /translation="MTPNRIAPPTNIPPWAKALVRLLDRAFVLPGTQIEVGLDPILGL
                     FAPGAGDLVGALATLSLLHLGFKLRVPRVILLRMLLNVAIDAIVGSIPVLGDAFDLFY
                     RAAQKNLALIEKHGGAAPQNPSFGDYAVVGLAVVVAIALLALPVLAGFGLLYLTARWV
                     GR"
     gene            complement(2201..2914)
                     /locus_tag="VFQ35_15435"
     CDS             complement(2201..2914)
                     /locus_tag="VFQ35_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932095.1"
                     /translation="MPDPPRKLPPHGFILAAVALLGLVAPMLGGARLQTLISLLVGAT
                     AVGVSSHFGSSRRARARAALTKSPSGRIESRVGPQSHRIFDLPGAGIEPWRADAWVMG
                     IVASAALFLGLSAEALSRPINFLLVLVSGALSLRLLTVNSDHIRLEISEHGYWIQAHE
                     GGRVIRRSGLGALFAELLPEAVVLWSREGRIGVLRGELEPAERAWLAEQLPIGASSER
                     SAPDPASRQVEQPKPGEDG"
     gene            2947..3630
                     /locus_tag="VFQ35_15440"
     CDS             2947..3630
                     /locus_tag="VFQ35_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932096.1"
                     /translation="MQSETNANPETGVARIDLVRNTGRMTSRVLAFALAWAFCCGCSS
                     DHHADDENPSLSDVVFVGGTTDEALSVMLDATPKDVASERVTFVSPEPSATLSKAAPV
                     AIQFQGAHARLQAPVDERPAQRPSLARRALWDLEQLLKPIGVAHAHGAPFNGTGYFLV
                     FEDAAHQSRLRVFTDQTAYTPDSAGWGAIAAGQQPIKLSVTSAVFEENAVLGDGGPFA
                     AGSIEFSVR"
BASE COUNT          766 a         1423 c         1284 g          665 t
ORIGIN      
        1 cttccctctg agtcgaaacg cgagcgctcg ccgtcgtggg actctgtctc cgcggcggca
       61 aaactgcgcg cagagccggt cgctcccgcc gcgaccgatc tcagcgcgag cgcacaactt
      121 ccaacgccgc cggcgccccg ccagccaact ccctggtttt cgcggctggt cctcatcggt
      181 ggcgcgatgt tggtgatggg tggctccttg gccgtcgcgg tgcgtctgtt catgacgcac
      241 gacgcacagg tcgaaaaacc accagcgcca ccgcccgaag cgagtgcggt ggccgcacct
      301 tcggaggctc ctccagactc gcccgtggca tccgcggagt cacccgcccc gccgttgctg
      361 gagagcgccg cggccgtacc gccggcgcca ccagtttcct ccgcggcgag cgcagcgccc
      421 cccgccccgc ccgcagccga acccgacgca ccgaagcaag ccgcagcgct cgctggcgtc
      481 cccattcgcg acgtcaccgg tggtccggtt gggcgcgccg tgatcggttc ttatcggctg
      541 ctcgcccgcg aagccgccgc cggtcgagac ttttacggcg ccgccaaaga atgcgccgac
      601 gtggggatgg ccctctgcag cgaagcgcag tggatgcatg cgtgcagcgc cttccccgag
      661 ctcggcgcgg ttgcaagctg gaccagcacc ttgaaggggg aacacgtcgt ggttcgcggc
      721 ggccaggact gcgccgccga gagcaaggcc gcgccggtgg agcgttcgct gaaccgcacc
      781 gggatgtgct gcgaccgcgc catcgccatg accaccgaca accttcagaa gaactacctc
      841 acgaccaccg gtgagcgagt gctcgcgatc gagcgcgcct tgaaccagcg caacatgagc
      901 agcctactcg agctgtcgga gtcgtcggtc gcagtcgacg accaaccgaa gagccccgct
      961 cagctacgcg cgctcgtcga gagcgctttc aagagcgccc ccggcattta cgtgctgaac
     1021 gacacttgcc acttctcggt gcaagccaaa aagatcacga agggcactcg tcggaagaag
     1081 aaggtcgtgt accagacgca gggctggacc gccgagtgtg atcaaacggc gatcgtgcct
     1141 tccacgagcg tatcggcgcg gaaggtcacg tacgggttca gcgcgtcgag caagctgcgc
     1201 gagatcacga ccaagccgga agagtgagag aaagtcagaa gaggcgcgtc gtatcgagga
     1261 gcagcgtcac gggtgcttcg ttcacgagcg acactcgcat ctcggttcgg aagcggcccg
     1321 tttcgaccgc gacaccgagc tcgcggcagg cggagcaaaa tgtttcgaag agctgctcgg
     1381 cggtctcggg tgccatcgcg tgcgcgaagc tcggccggcg acccttgcgg cagtctccga
     1441 gcagcgtgaa ctgcgagacc gcgagcacct ttcccccaac cgcggcaagc ggcaggttca
     1501 tcttaccggc ttcgtcctcg aagattcgaa gcccgacgac cttgtcggct agctcccggg
     1561 cgctcgcggc gtcgtcctcg cgagcgaccc cgacgaagac gcatacgccc cgctcgatcg
     1621 agccgaccac gacgccgtcg accctcacat ctgcgctcga aactcgctgc actacggctc
     1681 gcatcggcca gagtctaaag cgacggcggc gcgaagcctc ccaaatcgcc gcttgcacgg
     1741 cggcgccggc ccgccacatt ctcccgcgcc aatgaccccg aaccgaatcg cgccaccgac
     1801 gaacatcccg ccgtgggcga aggcgctggt tcgcctgctc gatcgcgcct tcgttttgcc
     1861 cggcacccaa atcgaagtcg gcctcgaccc gatcttgggc ctcttcgcac cgggcgccgg
     1921 tgatctggtc ggagcgctcg ccacgctctc gctcttgcac ctcgggttca agctgcgtgt
     1981 cccgcgcgtc atcttgctgc gcatgctcct gaacgtcgcc atcgacgcga tcgtcgggag
     2041 tattccggtg ctcggagatg cgttcgacct gttctaccgc gcggcccaga agaacctcgc
     2101 tctcatcgaa aagcacggcg gtgccgctcc gcaaaacccg agcttcggcg actacgcagt
     2161 cgtcgggctc gcggtcgtcg ttgccatcgc gctgttggcg ctacccgtcc tcgccgggtt
     2221 tgggctgctc tacttgacgg ctcgctgggt cgggcgctga tcgctccgag ctcgccccta
     2281 tcggcaactg ctctgctagc cacgctcgct ccgccggctc gagctcgccg cgcagcacgc
     2341 cgatgcgccc ttcgcgagac cacagcacga ccgcctccgg caagagctcc gcgaacaagg
     2401 cgcccaaccc cgagcgacgg atcacgcgac cgccctcgtg cgcttgaatc caatagccat
     2461 gctcggagat ctcgagccgg atgtgatccg aattgacggt caggagccgc agtgaaagcg
     2521 caccgctgac gagcaccaag aggaagttga tcgggcgaga gagcgcctcc gcgctgagcc
     2581 ccaagaacag tgccgccgac gcgacgatgc ccatgaccca agcgtcggcg cgccacggct
     2641 cgatgcccgc accaggcaga tcgaaaatgc ggtgactctg cggaccgact cgcgactcga
     2701 ttcggccact cggggacttt gtcaacgcgg cacgcgcccg tgctcggcgc gagctaccga
     2761 agtgcgacga gacgccgacc gcggtagccc cgacgagcag tgaaatgagc gtctgcaacc
     2821 gtgccccacc gagcatcggc gcaaccaaac ccaagagtgc gacggcggcg agaatgaaac
     2881 cgtgcggcgg tagctttcga ggcggatcag gcacggcttc gattgtggca ccgtttcgag
     2941 tgcacgatgc aatccgaaac gaacgcgaac cccgaaacgg gcgttgcgcg gattgatttg
     3001 gtgcgtaaca ccggacgaat gacgagtcgt gttcttgctt tcgcgctcgc gtgggccttc
     3061 tgctgcggtt gttcgagcga tcatcacgct gacgacgaga acccgtccct gagcgacgtc
     3121 gtcttcgtag gaggcacgac cgacgaagcg ctgagcgtca tgctggacgc aacgccgaag
     3181 gacgtcgcga gcgagcgggt aaccttcgtg agccccgagc cgtcggccac gctgtcgaag
     3241 gccgcgccgg tcgcaatcca atttcagggc gctcacgctc ggctgcaagc accagtcgac
     3301 gaacggcccg cgcagcgtcc ttcgctcgca cgccgggctc tctgggacct cgagcagttg
     3361 ctcaagccga tcggcgttgc gcacgcgcat ggggcaccat tcaatggaac cggatatttc
     3421 ctggtcttcg aagacgctgc tcaccaatcg agactacggg ttttcaccga ccagactgct
     3481 tacactccgg acagcgcagg atggggcgca atcgccgccg gccaacagcc gatcaagctc
     3541 agcgtgactt cggccgtgtt cgaggagaac gcggtgctcg gcgatggtgg gccctttgcg
     3601 gccgggagca tcgagttcag cgtccgctga gccgactccc gggcgagagc gaaaatgcgc
     3661 gtgcagccta tttaaggaga ggctgcaccc agaaccgaaa atgtcatcgt cacacgaacc
     3721 gatgcaccga attgcgcaga ggtcgccttg acgaacgtca ttgccaaagc gagactcctg
     3781 gcgttgccga ccagtcggta tacgcacgcg tgcgcctgca atcgagttgc attccgagct
     3841 tcgctcgtga atcggatttc gacgttctcg tgacaaacgc agctttcgat tgaaagagtc
     3901 gcgctctctc aaataaatgc gcactgcatg cgggcattcg aatcagcata gctgtgcggc
     3961 accgtgtgcg tgccgtgccg cgctacctac gaccagaccc atgtgccccg aagccgctca
     4021 ctcccagaca gcgaatagcg tgagcggcta ctgagaaaga ctgaaccgaa gcgcttcgac
     4081 gacgcgcaac cgtgtctctc gcagcacggc cgcctcaagt atgcggccgc gccgcccg
//
LOCUS       DASTKS010000745         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_565217, whole genome shotgun sequence.
ACCESSION   DASTKS010000745 DASTKS010000000
VERSION     DASTKS010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565217"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFQ35_15445"
     CDS             complement(<1..346)
                     /locus_tag="VFQ35_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9932097.1"
                     /translation="MVHDSRSDDPRSALDELTRLRAENARLGRELDEQRERFAHVMQN
                     GLVGYLVSSFEENRFIDANRAFCDFIGMSRDELLATDPYQFWLDTTFSDDKDAEHREL
                     QRVADGSVDDYRI"
     gene            complement(432..803)
                     /locus_tag="VFQ35_15450"
     CDS             complement(432..803)
                     /locus_tag="VFQ35_15450"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET9932098.1"
                     /translation="MFIAKHFLGLPVDEADDIRRNLSLVLSTKRGTGYFLKNFGLSDA
                     VFRTPEETVTVLGEELKENIRLYEPRVELIKINEVYDDEGRRMSLVVVLQRRATKTRM
                     HLNVDLKTGSFDVVPAQAPKP"
     gene            complement(811..1818)
                     /locus_tag="VFQ35_15455"
     CDS             complement(811..1818)
                     /locus_tag="VFQ35_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932099.1"
                     /translation="MTPGALFPAKELTPIEARIEAEATRFDLRPLLSLLSQIGYGREA
                     ILFESTESGRSPSLVQAVRFVKSPPVTAIISVHVGLLGDNTLLPSYFFYLIERSPDPA
                     KFFDFLRFFDHRLIENMFAVLHPEIGEDERSPMEGSSTGKSWLFGNYRGVLRSFFRMS
                     APNSPGTLHSLVQLYFPEFRVRVSRQPFAEETDRHACLTGVSKLDGSAILGATYVTEM
                     PGFLVDLIVDDEVDLSGREQADIVKSRLEERLLPLLTSFHLPLTIRLVVLWHSSAAYV
                     DDPSAEDKSFLGYNRLHGARNRPEIKHTTVMYRGLTDEFGQRETHDLKSLVPRAAAGA
                     R"
     gene            complement(1822..2790)
                     /locus_tag="VFQ35_15460"
     CDS             complement(1822..2790)
                     /locus_tag="VFQ35_15460"
                     /inference="COORDINATES: protein motif:HMM:NF041244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein IglI family
                     protein"
                     /protein_id="HET9932100.1"
                     /translation="MGADFSLLLAPFAPSGAVSDAVVERLEAVERAAEKEDYALARDL
                     AAELGRDGIHDIRPISYWLFQEFREEGFEGIAKSLEALENVLGPSLAALGPERRKEEY
                     VTRRLVWLFDKISAAVEYHEKYATAQWDSLKRTLVPETLARIAAAGERVTVLLAAQAE
                     AMRALSQLLSRARSYRLEAPKPPEPTAESKSASVVPASPADPTTTAAAVPAIPTPFPA
                     KEGLSRVELLVAYPFVELCRRLKAFESLVEKHELRKAAVVAEDIAYAVEHFDPLANFP
                     ELFARHAALLCKHAARLSEFDAERQSATWLALARYARVDLDAFVEG"
     gene            complement(2793..3179)
                     /locus_tag="VFQ35_15465"
     CDS             complement(2793..3179)
                     /locus_tag="VFQ35_15465"
                     /inference="COORDINATES: protein motif:HMM:NF025472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HET9932101.1"
                     /translation="MAQLVVAGAILKCTMGAAPSSLAVLPQNRVMASTPTANIMDHVP
                     FLNVLPFGMCNSMANPVVAAATAAALGAFTPMPCIPATAAPWMPGVPTVLVGNLPAVD
                     SGCQLMCSFGGAISVVNPGQLQATGG"
     gene            complement(3192..>4081)
                     /locus_tag="VFQ35_15470"
     CDS             complement(3192..>4081)
                     /locus_tag="VFQ35_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932102.1"
                     /translation="SAWFAGAAFVELSAERVARQPELLSAYQVNFLGVRPELRDAFNE
                     RAQWPAPTVRGWFRNLTDALVLTPWDRFGKLASAAGVFGFNTAFSESAGGALLRGPTL
                     NDEPSLRAWPVAGRPWLLTEVLGGTLESLHDSGIYTPLHAEKAIEGAPRLILSRGERG
                     YECSGALDLGPDACTYPIEEVSRVAERHPAVTYASVVVSGRGINSANVTLLAFCEDPS
                     AVDRTDVLRWLEREMGARLLPGRVEVFALLPRLGKEGVDHGWCRSQYLSGALTKKASD
                     PLFTMISRLSYLMSAPPGA"
BASE COUNT          755 a         1313 c         1338 g          675 t
ORIGIN      
        1 ggatgcgata gtcgtcgacg ctgccgtccg ccacgcgctg gagctcgcgg tgctcggcgt
       61 ccttgtcgtc ggagaaggtc gtatccaacc agaactggta agggtccgtc gcgagtagct
      121 cgtcgcggct catgccgatg aagtcgcaaa aagcacgatt tgcatcgatg aagcggttct
      181 cctcgaacga cgacaccagg tagccgacga gcccgttctg catgacgtgc gcgaagcgct
      241 cacgttgctc gtcgagctcg cggccgagcc gcgcgttctc ggcgcgcaac ctcgtgagct
      301 cgtcgagggc ggagcgcgga tcgtcggagc gcgagtcgtg aaccatgggg acttcggacc
      361 tttaccgcct gctcgcgaga ccgcaagccc ggctcaggtc ggcgcccttc ccgagcgccg
      421 gcaaggatgg atcaaggctt gggcgcttgc gccggcacga cgtcgaagga accggtcttg
      481 aggtcgacgt tcaggtgcat tcgcgtcttg gtagcgcgcc gctgaaggac gacgacgagg
      541 ctcatccttc ggccctcgtc gtcgtacacc tcgttgatct tgatgagctc gactcgcggt
      601 tcgtagaggc gaatgttctc cttcagctcc tcgccaagca ccgtgaccgt ctcctccggc
      661 gtccgaaaaa ccgcgtcgct gagcccgaag ttcttgagaa agtaccccgt tccgcgcttg
      721 gtcgagagga cgagcgagag attgcgccga atgtcgtcgg cctcgtccac agggagcccc
      781 aagaaatgtt tcgcgatgaa catctgaaac ctagcgcgcc cccgccgcgg ctcgcggcac
      841 gagcgacttg agatcgtggg tctcgcgttg cccgaactcg tcggtgaggc cgcgatacat
      901 caccgtcgtg tgcttgattt cgggccgatt gcgggcgccg tgcaagcggt tgtagccgag
      961 aaagctcttg tcctcggcgc tcgggtcgtc gacgtaggcg gcgctcgagt gccaaaggac
     1021 gaccaaccgg atcgtgagcg gcaggtggaa cgacgtgagc agcggcaaga gccgctcctc
     1081 gagccgagat ttgacgatat cggcttgttc gcggccgctc aggtcgacct cgtcgtccac
     1141 gatgagatct accaagaagc ctggcatctc ggtgacgtag gtcgcaccga ggatggcgct
     1201 gccgtcgagc ttcgagacac cggtgaggca agcgtggcga tcggtctcct cggcgaaggg
     1261 ctggcgcgaa acacgcaccc tgaactccgg gaagtagagc tgtacgagcg aatgaagcgt
     1321 tcctggcgag ttcggcgcgg acatgcggaa gaacgagcgc agcacgccgc gatagttacc
     1381 gaaaagccag ctcttaccgg tcgagcttcc ttccatcggc gagcgctcgt cttcgccgat
     1441 ttcggggtgg agcacggcaa acatgttttc gatcagacga tgatcgaaga agcgcagaaa
     1501 gtcgaagaac ttcgccgggt cggggcttcg ttcgatcaga tagaagaagt agcttggtag
     1561 gagtgtgttg tcgcccaaga ggccgacgtg cacgctgatg atggcagtca cgggaggtga
     1621 cttgacgaac cgcaccgcct ggacgagtga cggtgagcgc cccgactccg tactctcgaa
     1681 aaggatcgct tcgcgcccgt atccaatctg cgaaagaagc gaaaggagtg gcctcagatc
     1741 gaagcgcgtc gcctccgcct cgatgcgcgc ctcgatggga gtgagctcct tcgctgggaa
     1801 gagtgcgccc ggagtcaacg ctcagccctc gacgaaggca tccaggtcga cgcgcgcgta
     1861 gcgagcgagc gcgagccacg tcgccgactg ccgctcggcg tcgaactcgc tcaggcgcgc
     1921 agcgtgcttg cagagcaacg cggcgtgacg cgcgaacagc tccgggaagt tcgcgagcgg
     1981 gtcgaagtgc tcgaccgcat aggcgatgtc ctcggcgacg accgcggctt tacgaagctc
     2041 gtgcttctcg accaggctct cgaacgcctt gagacgccga cagagctcga cgaacgggta
     2101 cgcgaccaag agctcgacgc gcgagagacc ttctttcgcg gggaacggcg tcggaatcgc
     2161 tggcaccgcc gccgccgtcg tcgttggatc cgcagggctc gcaggaacga cagaggcgct
     2221 cttcgactcc gcggtgggct cgggcggctt cggagcctcg agacgatagc tgcgcgcgcg
     2281 tgagagcagc tgcgagagcg cgcgcatcgc ttcggcctgg gccgccaaga gcaccgtcac
     2341 tcgctcgccg gcggcagcga ttcgcgccag tgtctcgggc actagcgttc gcttcaatga
     2401 gtcccactgc gcggtggcgt acttctcgtg atactcgacc gccgcggaga tcttgtcgaa
     2461 cagccagacg agccgccgtg taacgtactc ctctttgcga cgctcggggc cgagcgcggc
     2521 gaggctcggt ccgagcacgt tctcgagcgc ctccaagctc tttgcgatgc cctcgaaccc
     2581 ttcttcgcga aactcttgaa agagccagta agatatcggc cgaatgtcgt ggatgccgtc
     2641 ccggccgagc tccgcagcga gatcacgcgc aagcgcgtag tcttccttct cggcggcgcg
     2701 ctcgacggcc tccaggcgtt cgaccacggc gtcggagacg gcgccggacg gcgcgaacgg
     2761 cgccagcaag aggctgaagt cggcgcccat ggtcagcctc ccgtcgcctg cagctgcccc
     2821 gggttcacga ccgagatggc gcccccgaag ctgcacatga gttggcagcc agagtccacc
     2881 gccggcaggt ttccgaccaa caccgtcggc actcccggca tccacggcgc ggccgtagcc
     2941 ggaatgcacg gcatcggagt gaacgcaccg agcgcggccg cggtcgccgc ggcgacgacc
     3001 gggttcgcca tgctgttgca catcccgaaa ggcagcacat tgagaaatgg cacgtgatcc
     3061 atgatgttcg cggtcggagt ggacgccatc acgcggtttt gcggcagcac cgcgagcgag
     3121 gagggcgccg cccccatcgt gcacttcaaa atcgcaccag ccaccaccaa ttgagccatg
     3181 atctaccgcc ttcatgcgcc ggggggcgcg gacattaggt aactcaaacg cgaaatcatc
     3241 gtgaagagag gatcgctcgc cttcttcgtg agcgcgccgg acaggtactg agagcgacac
     3301 cagccgtgat cgacgccctc tttgccgagc cgagggagca acgcgaatac ttcgactcgc
     3361 cccggcaaga ggcgcgcgcc catttcgcgc tcgagccagc gtaaaacatc ggtgcggtcg
     3421 accgcactcg gatcctcgca gaaggcgagt agcgtcacgt tcgccgagtt gatgccccgc
     3481 ccagatacga cgacgctcgc gtaggtcacg gccgggtgcc gctccgcaac ccgactcacc
     3541 tcctcgatgg gataagtgca agcatcgggt ccgagatcga gtgcaccgct gcattcgtag
     3601 ccccgttcgc cgcgcgacaa gatgagccga ggggcgccct cgatcgcctt ttcggcgtga
     3661 agcggcgtgt agatccccga atcgtgcagc gactccagcg tgccaccgag cacttcggtg
     3721 aggagccaag gcctgcccgc gaccggccac gcgcgcagac tcggctcgtc gttgagggtc
     3781 ggtccgcgca gcagggcgcc gcctgcgctc tcggaaaacg cggtattgaa gccgaagacg
     3841 ccggccgcgg acgcgagctt tccgaagcgg tcccacggag tcagcacgag cgcgtcggtg
     3901 aggttccgaa accaaccccg taccgtcggc gccggccatt gtgcgcgctc gttgaaggca
     3961 tcgcgaagct cgggccgcac gccgaggaag ttcacctggt aggcgctgag tagctctggc
     4021 tgccgtgcca cgcgctcggc actcagctcc acgaaagccg ctccggcaaa ccacgcgctg
     4081 a
//
LOCUS       DASTKS010000746        10251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_565284, whole genome shotgun sequence.
ACCESSION   DASTKS010000746 DASTKS010000000
VERSION     DASTKS010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565284"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VFQ35_15475"
     CDS             complement(<1..1362)
                     /locus_tag="VFQ35_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HET9932103.1"
                     /translation="MPRRHFSQIGLRASTPPTSVDEAEPEVDPMGYPRPQFRRREWFT
                     LNGEWDFALDADHEFTKPNQITWASKITIPFAPETQLSGIGDTSLFRACWYRRTFQAP
                     ELREKERLILHFGAVDYQANVWVDGTLVTQHEGGYTPFWVDITEALSPSADHEIVVLA
                     LDDPSDLAKPRGKQDWQLNPHSIWYPRTTGIWQTVWMERVPAVRLASLQWTPNLASWE
                     IAIDTAVHGHVTEKLSVSVRLKVDDLVLAHDTYTVVASEVHRRIALSDPGIDDFRNEL
                     LWSPERPKLIEAEVELLDSSGQRIDRVTSYTALRSFSVQGDRFLLNGRPYPLRLVLDQ
                     GYWDGGGATAPSDAAYRRDVELVRQMGFNGVRKHQKIESPRYLYWADRLGLLVWEEMP
                     SAYRFTKDSVARLTREWLQVLRRDASHPCIMAWVPFNESWGVPNLPDSLPERNFVRAL
                     YYLT"
     gene            1754..2944
                     /locus_tag="VFQ35_15480"
     CDS             1754..2944
                     /locus_tag="VFQ35_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9932104.1"
                     /translation="MENYDIVCLSHLRWSFVFQRPQHLLSRCALERRVFFVEEPIFSA
                     EHCGPARLEATTSSEGVVVVVAHFADGISPEEVDHTYGQLLREYLAREDVSDYVLWYY
                     TPMALPLAEQLAPLAVVYDCMDQLSAFKNAPPLLTRRERELFARADVVFTGGHALYEA
                     KKQQHPNVHAFPSSVDVPHFAKARVPQLDPEDQRNIPGPRLGFFGVIDERMDLELLTR
                     VAAARPYWQLVMLGPVVKIDPADLPNAPNIHYLGGKSYAELPAYIAGWDVALLPFARN
                     ESTQFISPTKTPEYLAAGKPVVSTSIRDVVRPYQRLGLVRIADAAEAFVSACEEAMCE
                     PVGQRLLKADAYLSHLSWDRTWREMRTRMEQAIERRSMKSAPQLRVAESSDEALLDVA
                     LGGE"
     gene            2944..4161
                     /gene="glf"
                     /locus_tag="VFQ35_15485"
     CDS             2944..4161
                     /gene="glf"
                     /locus_tag="VFQ35_15485"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HET9932105.1"
                     /translation="MFDYLVVGAGFAGSVLAERLASDWGKKVLVVDRRPHVAGNAYDC
                     YDDAGILIHKYGPHIFHTNSREVFDYLGRFTEWRPYQHRVLASVDGQLVPIPINLDTI
                     NTLYGTNMSATELEAFFEKMAEKRHPVRTSEDVVVNKVGRELYEKFFRGYTRKQWGMD
                     PSELNSSVTARVPTRTNRDARYFTDTYQAMPLRGYTRLFEKMLDHPNIHVMLNTDYRS
                     IAGMIPYREMVYSGPVDEFFDFKLGKLPYRSLHFSHETLRQSQFQAVGTVNYPNENAY
                     TRISEFKHMTGQRHEYTSVVYEYPRAEGDPYYPVPKPENDELYAKYKAMADATANVHF
                     VGRLATYKYYNMDQVVAQALTLYKKLREHAEPVLELNDVSNNHRVHLEARNAGESGTL
                     AVGRVRVHHQSGG"
     gene            4097..5395
                     /locus_tag="VFQ35_15490"
     CDS             4097..5395
                     /locus_tag="VFQ35_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9932106.1"
                     /translation="MQANQVLSLWGGFECTINRVGDRYHDQLEYSGHDERIEDLDLIA
                     DLGIRCLRYPVLWERSEPALGVERFGWADQRLARLQELGIEPIVGLLHHGSGPRYTSL
                     LDPNFPTKLAGYARRVAERYPTLERFTPVNEPLTTARFSGLYGHWYPHGRDDRTFVKS
                     LLNQCKGTCLAMQEIRNVNPGAKLVQTEDMGFTRSTQELTYQAEFDNQRRWLSLDLLA
                     GHVHAEHPLYDYLLDAGASRRDLDFFLEHPCPPDLVGLNYYVTSERFLDGRVELYSPE
                     IVGGNGRDRYVDVEAVRVCADGLLGPTPLLLEASARYGRPVAITEAHLGCASAQQIRW
                     LEYVWNAALAARELGADVRAVTVWALLGAYGWDKLATQGPCSYEAGTFDVSSGVARET
                     PLTHFVRMLARGEAHETEVGWWELEARLLYEPHESESRAA"
     gene            5694..6491
                     /locus_tag="VFQ35_15495"
     CDS             5694..6491
                     /locus_tag="VFQ35_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9932107.1"
                     /translation="MTGSGGATSGGSASGGVSSGGASSGGASTNGGTASGGASSGGAS
                     IGGASGGGTSSTGSGGSSAGGRGGAGGVGGAAGGASGASGTTGGSGGGSGTSFTLTSP
                     SWTSQPDCSPDKKAACGVFPKESLGASIGGTNKSPELVWTEGPSGTQSYAIVMHDLTY
                     LMNGKPFVHWVIWNIPGSTRMLPAGLDSAAMPSVPAGASQKSFQGNGFQGSGKCGNVY
                     ELVLYALPTATISPTMTSQTTVADALDATKAPTTTLRVRSGAPGCTE"
     gene            complement(6557..6811)
                     /locus_tag="VFQ35_15500"
     CDS             complement(6557..6811)
                     /locus_tag="VFQ35_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932108.1"
                     /translation="MNTAVLSIACSIGLATLTACGDAGENGKSEPTPTPEEAGKLQGI
                     EGTGLQGIEGTGRKSSPRRDTQGIQGSGRTLQGIQGTGRQ"
     gene            6950..8098
                     /locus_tag="VFQ35_15505"
     CDS             6950..8098
                     /locus_tag="VFQ35_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HET9932109.1"
                     /translation="MKGSSLRAPPIECRDPESFGGFTVRERFPKIMAQARAGLEPARA
                     ADARFDELLDDVVSGRAIRMAHFAHQTEYWRSYLQALRGRSWSELSFFETEFCFYQAI
                     NSLAGAFDSEVDVFAHVKSEALEAAVRSLESRPYEFLSKTSLGELVMSATIGNVADAS
                     QLAAHGDVERIQSLIVDDSAGLSQLLSAPLGAVHWLLDNAGAELCADLALVDRLITTT
                     AARVVLHAKPWPMFVSDALLSDVDNSLARFSYSESSELRALGRRLQVQREAGRLSLRA
                     PSCWGEPRHFFEVPSDLAAELRSATVVVAKGDLNYRRFIEDRAWPADTPLSLANTSSF
                     SAYALRVLKSEALAGISTESARALSQAEPDYRISARHAMVQYLPCAVG"
     gene            complement(8153..9076)
                     /locus_tag="VFQ35_15510"
     CDS             complement(8153..9076)
                     /locus_tag="VFQ35_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932110.1"
                     /translation="MRKTKHYGALLTGMLSAVAWPRSGVAEVAGHSAIDSRAYSRPSP
                     ASLEPSSPRALARDGGVEARASLDFGGLLPLYHHIQLGRDGTEFDYVRDGGQDVIFPV
                     GRVSVELRSQRHHATFLYQPLALNSEVVLKRPLTVDGLTFPAETPMNLRYGFPFYRVS
                     YAYDFVSTSAWRVALGASFQLRDATISFTSADGAARRTRRDVGPVPALKGVVRYDAPS
                     GWFGAVEIDGFYAPIKYLNGGDSDVVGAIIDANVRAGYRLSPRVDGFLNLRWLGGGAE
                     GSSEPEDFSDGYNQNWLQFLLVTVGVDFALL"
     gene            9242..>10251
                     /locus_tag="VFQ35_15515"
     CDS             9242..>10251
                     /locus_tag="VFQ35_15515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9932111.1"
                     /translation="MSMFSRFLLRAVRAYRPTPPNGLALFALAGAAAFVGARKLMHRP
                     AVGARALVLGGSRGLGLLLAEELVARGSRVVIAARDDAELTEAVAAIEHSMAAEGRIS
                     AVRCDVEDEGDIALAVHTARERLGGIDVLVNNAGVIQVGPLEAMDVADFEHALRVHLR
                     APLIASLAVVEEMRARRAGRIINISSIGGLVPIPHMAPYVASKFALTGLSQGLQVALA
                     KDGISVTTVCPGLMRTGSSHRATFKSKHRSEHAWFSLGAATPLTSMNARRAARQILNA
                     AAAGKGLVVLSWQAKLLSSFHALAPGMSLRVLALVNRLLPAFGGIGKQALEGRYSASR
                     WAP"
BASE COUNT         1850 a         3187 c         3323 g         1891 t
ORIGIN      
        1 ggtgaggtag taaagggctc gcacgaagtt gcgctcgggc aagctgtcag gcaggttggg
       61 cacgccccac gactcgttga atggaaccca cgccatgatg cacggatggc tcgcgtcgcg
      121 gcgcaacacc tggagccact ctcgagtgag gcgagcgacc gagtctttcg tgaagcgata
      181 cgcactcggc atctcctccc agaccaagag cccgagtcga tccgcccaat ataggtagcg
      241 aggcgactcg atcttttgat gtttccgtac gccattgaaa cccatttgcc ggaccaactc
      301 gacgtcgcgc cggtaggcgg cgtcgctcgg cgcggtggct cctccgccgt cccagtagcc
      361 ctgatccaag accagcctga gcgggtatgg gcgcccgttc aaaaggaacc gatctccttg
      421 caccgaaaag gagcgcagcg ccgtatagct agtcacccgg tcgatacgct gccccgacga
      481 gtcgagcaac tcgacctcgg cttcgatcaa cttcggccgc tcggggctcc acaagagctc
      541 gttgcggaag tcgtcgatgc ccggatccga cagggcgatg cgtcgatgca cctcgctcgc
      601 gaccaccgtg tacgtgtcgt gagccaaaac gagatcgtct accttgaggc gtacgctcac
      661 cgagagcttt tcggtgacgt ggccgtgcac ggcggtatcg atggcgatct cccagctagc
      721 caagttgggc gtccattgca ggctggcgag tcggaccgcg ggcacccgct ccatccaaac
      781 cgtctgccaa atgcccgtcg ttcgcggata ccaaatcgag tgcggattca gctgccaatc
      841 ttgcttcccg cgcggtttgg cgagatccga tgggtcatcc agggcgagga ccacgatttc
      901 gtgatcggcg ctcgggctga gcgcttccgt gatgtcgacc caaaacggcg tgtaaccacc
      961 ttcgtgttgc gtgacgagcg tgccgtccac ccagacgttg gcctggtagt cgaccgcgcc
     1021 gaaatgcaga atcagccgct ccttctcgcg aagctcgggt gcctgaaaag tgcgacggta
     1081 ccagcacgct ctgaagaggg acgtgtcgcc gatgccgctc agttgagtct cgggggcgaa
     1141 cgggatcgtg atcttgctcg cccacgtgat ctggttcggc ttcgtgaact cgtggtcggc
     1201 gtctagcgca aagtcccact caccgttcag cgtaaaccat tctcgtctac gaaattgcgg
     1261 acgaggatag cccattggat cgacctcggg ttcggcctcg tccaccgatg tcggaggggt
     1321 cgaggctcga agaccaatct gcgagaagtg ccgacgcggc atgcaatccc ttcctcagag
     1381 acggacagct gatcatgaat cgcggacaga accttccgtc taaagtccgt ggttcgcccg
     1441 acgagcgacg cgtgcagaac gcgtaccact aggcccgctg agtgcgtcct cgcacggatc
     1501 aatcacgaat gagcgcgttt tatcctgcga gatctgctcg ttgccgcgca aagcccttgg
     1561 ttcgcgctcg atcggtggct cgcgctctcg aaggaacgcc ccacgtgcgc cgcatttgac
     1621 gttggtgcgc tgccgttcag aagcattctc ctcggcagat caatcacacc gtttcgttgg
     1681 tatgagaact gctaggtgcg agttctaaca accctaaagg cgttggcgct gtcacgccgg
     1741 agagattcca taaatggaga actacgatat cgtgtgcctc tcgcaccttc gttggagctt
     1801 cgtgtttcag cgtccccagc acttgttgag ccgctgtgcg ttggagcgac gcgtgttttt
     1861 cgtggaggaa cccatattct ctgcggagca ttgcggcccc gctcgcttgg aggcgacaac
     1921 aagctccgaa ggcgtcgtcg tggtcgtggc gcacttcgct gacgggattt cacccgagga
     1981 ggtcgaccat acttacggac aactgctgcg cgaatacctc gcgcgtgaag acgtgagtga
     2041 ctacgtgctc tggtactaca cgccgatggc ccttccgctc gccgagcagt tggcgccgct
     2101 cgcggtggtc tacgattgca tggaccagct ctctgccttc aagaacgctc ctccactgct
     2161 gacgcggcgt gaacgagagc tcttcgcgcg ggccgacgtc gtctttaccg gcggacacgc
     2221 gctctacgaa gcgaagaagc agcagcaccc gaacgtgcac gccttcccga gcagcgtgga
     2281 cgtgccgcat ttcgcgaagg cgcgcgtgcc gcagctcgac ccagaagacc agcgcaacat
     2341 cccagggccg aggcttggct tctttggcgt catcgacgag cgcatggatc tcgagctgtt
     2401 gacgcgagtg gcggcggcgc ggccgtactg gcaactcgtg atgctgggtc ccgtcgtgaa
     2461 gatcgatcct gcagatttgc cgaacgctcc taacatccac tacctcggcg gtaagagcta
     2521 cgccgagctg ccggcgtaca tcgcgggctg ggacgtcgcg ctcctgccct tcgccagaaa
     2581 cgagtccacg cagttcatca gtcccacgaa gacgccggaa tatttggcgg cggggaagcc
     2641 ggtcgtgtcg acctcgattc gcgacgtcgt gcggccctac cagcggctcg gcctcgtgcg
     2701 tatcgccgac gcggctgaag cgttcgtgtc ggcctgcgaa gaagccatgt gcgagccggt
     2761 cggtcaacgt ctgctgaaag ccgacgcgta cctgtcgcac ctgtcttggg atcggacctg
     2821 gcgagaaatg cgcactcgca tggagcaagc aatcgagcgc cgaagtatga agtcggctcc
     2881 gcagttgcgc gtggcggagt cgagcgacga agcgttgctc gatgtcgccc tgggagggga
     2941 atagtgttcg actacttggt cgtgggcgca ggcttcgcgg gtagcgtgct ggccgagcgc
     3001 ttggcgagtg attggggtaa gaaggtgctc gtcgtcgaca ggcgcccgca cgtggccgga
     3061 aatgcctacg actgttatga cgacgcgggc atcctgatac acaagtacgg tccccacatt
     3121 ttccacacga actcgcgcga agtgttcgac tacctcgggc gcttcaccga gtggcgaccg
     3181 taccagcacc gcgtgttggc gagcgtcgat ggccagctgg tcccgattcc gatcaacctc
     3241 gacaccatca acacgctgta cgggacgaac atgtctgcaa ccgagctcga ggcattcttc
     3301 gagaagatgg cggagaagcg tcatcccgta cgcacgagcg aggatgtggt cgtcaacaag
     3361 gtggggcgag agctgtacga gaagtttttt cggggctaca cgagaaaaca atggggcatg
     3421 gatccgtcgg agctgaacag ctccgtaacg gcgcgagtcc cgacccgtac gaaccgagac
     3481 gcgcggtact tcacggatac gtatcaggcg atgccccttc gcggctatac gcgcctgttc
     3541 gagaagatgc tcgatcaccc gaacattcac gtgatgctga ataccgacta ccggagcatc
     3601 gcggggatga tcccgtaccg cgagatggtg tactccggtc cagtcgacga gttcttcgat
     3661 ttcaaactcg gaaagctgcc ttaccgctct ctgcacttca gccacgaaac actgcggcaa
     3721 agccagtttc aggccgtcgg caccgtgaac tacccgaacg agaacgctta cacgcggatt
     3781 tcggagttca aacacatgac cggtcagcgg cacgaataca cgagcgtcgt gtacgagtat
     3841 ccgcgcgccg agggcgatcc gtactacccg gtgccgaagc cagaaaacga cgaactctac
     3901 gccaagtaca aggcgatggc agacgcgacc gcgaacgtgc acttcgtcgg acgcctggcg
     3961 acgtacaaat attacaacat ggatcaggtc gtcgcgcagg cgctgacgct ctacaagaag
     4021 ttgcgcgagc acgccgagcc ggtgctcgag ctgaacgacg tttcgaacaa ccacagagtt
     4081 cacttggagg cgaggaatgc aggcgaatca ggtactctcg ctgtggggcg ggttcgagtg
     4141 caccatcaat cgggtgggtg atcggtatca cgatcagctc gaatactcgg ggcacgacga
     4201 gcgcatcgaa gatctcgatc tgattgcgga cctcggtatc cggtgtctgc gctatcccgt
     4261 gctttgggag cgctctgaac ccgcgctcgg cgtcgagcgc tttggctggg ccgaccaacg
     4321 gctcgcgcgc ctgcaggagc tcggcatcga accaatcgtc gggctcttgc accacgggag
     4381 cggccctcga tatacctcgc tgctcgaccc gaattttccg acgaagctcg cgggatacgc
     4441 gcgtcgagtc gccgagcggt acccaaccct ggagcggttt acccccgtga acgagcccct
     4501 caccaccgcg cgcttcagcg gcctttacgg gcactggtac ccccacgggc gcgacgatcg
     4561 caccttcgtg aagagcctct tgaaccagtg caaaggcacg tgtttggcga tgcaggagat
     4621 ccggaacgtg aacccgggcg cgaagctcgt gcagaccgag gacatgggct tcactcggtc
     4681 cacccaggag ctcacttatc aagccgagtt cgataaccaa cggcgctggc tgagcctcga
     4741 cctactcgcg gggcacgtgc acgcagagca cccgctctac gactatctcc tggacgcggg
     4801 cgcctcgcgc cgagatctcg actttttctt ggaacacccg tgtccgcccg acttggtcgg
     4861 cttgaactac tatgtgacga gcgaacgttt cctcgatggt cgggtcgagc tctactcgcc
     4921 agagatcgtc ggcggtaacg ggcgcgaccg ctacgtcgac gtcgaggccg tacgcgtctg
     4981 cgccgacggc ttgttggggc ctacgccgct cttgttggag gcgagcgcgc ggtacgggcg
     5041 cccggtggcg attaccgagg cgcaccttgg ttgcgcttcg gcccagcaaa ttcgctggct
     5101 cgagtacgtg tggaacgcgg cgcttgcggc gcgagagctg ggcgccgacg tgcgcgcggt
     5161 cacggtttgg gcgctgctcg gcgcttatgg ctgggacaag ctcgcgaccc aaggcccgtg
     5221 cagctacgaa gcggggacct tcgacgtctc gagcggcgtt gcgcgagaaa ctccgctcac
     5281 tcacttcgtc cgaatgctcg cgcggggcga ggcgcacgaa accgaggtcg gctggtggga
     5341 gctcgaggcg cgcttgctct acgagccgca cgagtccgaa tccagagccg cgtgagcgag
     5401 tcgcatccgt tgccttcggc tgaaatgacg ccgttgcgcc ggtccgcgca ggccacaaaa
     5461 ttgcagcaga tcgaatgaca atatcctttc aactggcgtc cccacggctg cagcgcgcgt
     5521 gctaatgcgc gggcatgacg gaccgcatgc agatcttgcg atgggctcta cctactactt
     5581 tggtttcctg cttggcgatc gcagcgggat gcagctccaa cgagtcgaac tcgggagcag
     5641 gtggctcccc gggtaatggc ggagccgcga gtggtggcac tgcgagcggc ggcatgactg
     5701 gatcgggcgg cgcgacgtct ggcggtagcg cgtctggcgg cgtgagcagc ggcggcgcaa
     5761 gcagcggcgg cgccagcacc aatggaggaa cagcaagcgg tggcgcgagc agcggcggcg
     5821 cgagcatcgg cggtgccagc ggtggaggca cgtcttcgac gggctcggga ggctcgtcgg
     5881 cgggaggtcg tggcggtgcc ggtggtgttg gtggcgccgc gggcggtgcg agcggcgcct
     5941 cgggaacgac cggcggcagc ggcggcggca gcggcacgag cttcacgttg acgagcccca
     6001 gctggacaag tcagcccgat tgctcccccg acaagaaggc ggcgtgcggc gtcttcccca
     6061 aagagagctt gggcgcgagc atcggcggta ccaacaagtc gcccgagctc gtttggacgg
     6121 aagggcctag cggtacgcaa agctacgcga tcgtcatgca cgatctcacg tacctgatga
     6181 acggtaagcc tttcgtgcac tgggtcatct ggaacatccc tggttcgacg cggatgctgc
     6241 ccgcgggtct cgactcggcg gcaatgccga gcgtgcccgc tggcgcgtcg cagaagtcct
     6301 tccagggcaa cgggttccaa ggctctggca agtgcggcaa cgtctacgaa ctggtgttgt
     6361 acgcgttgcc taccgcgacc atttctccga cgatgacttc gcaaacgacg gtcgccgatg
     6421 cgctcgacgc gaccaaggcg ccgacgacga cgctgcgcgt gcggagcgga gcgccgggct
     6481 gcaccgagtg acacgctgag ttccgctggc gggcgcgcct cggcaccgtc gccgagcgcg
     6541 tccgcgcgac gttgctctat tgccgccccg tgccttgaat gccttggagt gtccgtccac
     6601 tcccttggat gccttgcgtg tcacggcgcg gagagctctt gcgtcccgtg ccttcgatgc
     6661 cctggagccc cgtaccctcg atgccctgga gttttcccgc ttcctccggc gttggcgtgg
     6721 gctcgctctt cccgttctcg ccggcgtcgc cgcaggcggt gagagttgcg agcccaatcg
     6781 agcacgcaat gctgaggacg gccgtgttca tggtcgacgc agtctacacc cgagtgtccg
     6841 cgaaggagca atccccaaca ggccgggcga cagatctcgt cggaagacgt aagtcgtgcc
     6901 cgtcgaaatc cgctccgtct cgcgcctctg agtggcgcaa ccaagcgaca tgaaaggttc
     6961 ttcgcttcga gcgccgccga tcgagtgccg cgatcccgaa agcttcggcg gctttaccgt
     7021 ccgcgagcga ttcccaaaga taatggccca agcgcgcgct ggtttggagc ccgcgcgcgc
     7081 cgcggacgcg cgcttcgacg agctcttgga cgacgtcgtc tcgggtcgcg cgatacggat
     7141 ggcgcacttt gctcaccaga ccgaatattg gcgatcgtat ctgcaagcgt tgcgcgggcg
     7201 cagttggtcg gagctgtcct tcttcgaaac cgagttctgt ttttaccagg cgatcaattc
     7261 gctcgcgggc gcgttcgact cggaggtgga cgtgttcgcg cacgtaaaat ccgaagccct
     7321 cgaggcggcc gtgcgctcgc tcgagagtcg gccctacgag tttctatcca agacttctct
     7381 cggtgagctc gtgatgagcg ccacgatcgg aaacgtagcg gatgcaagtc agctcgcggc
     7441 tcacggcgac gttgagagga ttcaaagcct gatcgtcgac gattcggcgg ggttgagcca
     7501 actgctttcg gcgcccctcg gggccgttca ctggctcctc gataacgccg gagccgagct
     7561 ctgcgccgat ctcgcgctcg tcgatcggct catcacgacg actgctgccc gagtagtcct
     7621 tcatgcgaaa ccctggccga tgttcgtctc cgatgcgttg ctcagcgacg tcgacaacag
     7681 tctggctcgc ttctcttact ccgaaagcag cgaactgcga gcgcttggac gtcgcctgca
     7741 agtgcaacgc gaagccgggc gactctcgct gcgtgcgcca tcgtgctggg gcgagccgcg
     7801 ccattttttc gaagtaccga gcgacctcgc cgcggagcta cgaagcgcga ccgtcgtcgt
     7861 tgcgaagggc gacttgaact atcgccgttt catcgaagac cgcgcatggc cagccgatac
     7921 accgctgtct ttggcgaata cgagttcctt ctcggcctat gcgttacggg tgctgaagtc
     7981 cgaggcgctg gcagggatct cgacggaaag cgcccgcgcg ctgagccaag cggagccaga
     8041 ttaccgcatc agcgcgcggc acgcgatggt gcagtacctt ccgtgcgccg tcgggtagcc
     8101 tggctctttc tgtttggacg gacggtgccc atcgagtgca gcctggccgc gttcacagca
     8161 gcgcaaagtc gacgcccacc gtcacgagca agaactgaag ccagttctgg ttgtagccgt
     8221 cgctgaagtc ttcaggctcc gaactgcctt ccgcgccacc accgagccaa cgcaggttca
     8281 agaaaccgtc gacgcgaggg ctcaggcgat agcccgcgcg aacgttcgcg tcgatgatcg
     8341 cgccgacgac gtccgaatcg ccgccgttga ggtattttat cggcgcatag aacccgtcaa
     8401 tctcgaccgc gccgaaccag ccggagggcg cgtcgtagcg cactacgcct ttgagagcag
     8461 ggacgggtcc gacgtcacgc cgtgtgcgcc gggccgcgcc gtccgccgat gtgaacgaaa
     8521 tggtggcgtc gcgcagctgg aacgaagcgc ccaaagcgac gcgccacgcg gaagtcgaga
     8581 cgaaatcgta ggcgtagctg acgcgataga aggggaagcc gtagcggaga ttcatcggcg
     8641 tctcggcggg gaacgtgagg ccatccaccg tgagcggtcg tttcagcacg acctcgctat
     8701 ttagtgcgag cggctgatag agaaacgtcg cgtgatgccg ctggctgcgg agctcgacgc
     8761 tgacgcggcc gacggggaag attacgtctt gcccgccgtc gcggacgtag tcgaactcgg
     8821 ttccgtcacg cccgagctga atgtgatggt agagggggag taaccctccg aagtcgagcg
     8881 atgcgcgcgc ttcgacgccg ccgtcgcgcg caagcgctcg cggggaggac ggttcgagcg
     8941 aagcggggga gggacgagaa tacgcgcggg aatcgatcgc ggagtggcca gcaacctcgg
     9001 cgactcccga cctcggccag gctacggcgc tgagcatgcc cgtgaggagc gcgccgtaat
     9061 gcttcgtctt gcgcatctcg gattggcatg gagccttcac tgcgtgcgtc aactgacgaa
     9121 ctcgtgcact cggggcgaga aaggtacgag gcacctttgc gcgtgcgcgt cagccgtgct
     9181 ctagcaaggt gcttcctctc gagcggcact cgtcttgctt tcaaccgcca cgaggtgcca
     9241 catgtcgatg ttttcgcgct tcctgttgcg ggcggttcgc gcatatcgcc ccaccccgcc
     9301 gaatggtctc gccctatttg cgctagcggg agcggcggcg ttcgtcggag ctcgaaagct
     9361 gatgcacagg cccgccgtcg gtgctcgcgc gctggtgctc ggcggctcgc gtggcctcgg
     9421 gttgctgctt gccgaggagc tcgtggcacg cggtagccgc gtcgtaatcg ccgcgcgcga
     9481 cgacgcagag ctgactgaag ccgttgcggc gatcgagcac tcgatggcgg cggagggacg
     9541 gatcagcgcg gttcgttgtg acgtcgagga cgagggcgac atcgcgcttg cggtgcatac
     9601 tgcgcgcgaa cggctcggcg gcatcgacgt actcgtgaac aacgcgggcg taattcaggt
     9661 cggacccctc gaggccatgg atgtcgccga tttcgagcat gcgctgaggg tgcacctgcg
     9721 cgcgcctctc attgcgagtc tcgccgtcgt cgaagagatg cgggctcgac gcgcgggccg
     9781 catcatcaat atttcgtcga ttggtggctt ggtgccgatc ccgcacatgg ccccctacgt
     9841 tgccagtaag ttcgccctga cagggctctc ccaagggctt caggtagcgc tcgcaaagga
     9901 tggcatcagc gtgacgacgg tctgtcctgg tctgatgcgc accgggagct cgcatcgtgc
     9961 caccttcaag agcaagcaca ggagcgagca cgcatggttt tcgttgggcg ccgctacgcc
    10021 gctcacgtcg atgaatgctc ggcgtgcagc gcgccaaatc ctcaatgcag cggccgcggg
    10081 aaaggggctg gtcgtgctct cgtggcaagc caagctgctc agctcctttc acgcgctcgc
    10141 tccgggcatg agtctgcgcg tcttggcctt ggtgaaccgg ctgctgccgg ctttcggcgg
    10201 catcgggaag caagcgctcg agggccgcta cagcgcttcg cgctgggctc c
//
LOCUS       DASTKS010000747         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_787926, whole genome shotgun sequence.
ACCESSION   DASTKS010000747 DASTKS010000000
VERSION     DASTKS010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787926"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFQ35_15520"
     CDS             <1..574
                     /locus_tag="VFQ35_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HET9932112.1"
                     /translation="SYSGVQRPVLFKLGPDGQGPRWVFSEPVGTAAEAYGAALQSNGK
                     FVTAGYGRPNAAATTSDFVSIRLNATGTLDTSYGVSGARWLDVGGFGDNARTVVVLGD
                     DRVLLAGGGRLTADDTDGAVAILTASGAPDATFSPAGCKTYDFGTPGDFLWAGALSPD
                     KKFVALVGITGVSSSSMLDNDSTFLLLPVE"
     gene            682..2019
                     /locus_tag="VFQ35_15525"
     CDS             682..2019
                     /locus_tag="VFQ35_15525"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9932113.1"
                     /translation="MPDEHVYTGRDTNGRGPSTASGGSTQFAKGGTGPSPDASGGRGG
                     ALPSLPPGGSAPYPDESGGASAGEGNVAHGGSPPVTEEPAPSFSKQALLGAIASCAAE
                     QIDEFAARADALTERATAHERAPGEESLAAVRAAWQTAMDSFQVLETLRFGPLARASE
                     DPKGRDLRDQMYAWPLGGRCNIETQLASQGYEGASFGASLINLRGLGALEYLLFYTAS
                     DNACPAYSPLNKDGTWAALGTDELARRKARYAAIVAADVARHAHDLYDDWKLDGDFRT
                     SFVSGAAYPSAQAALNAASNALFYVEVEVKDSKLGIPLGLSPDCTSATCPEALESRYA
                     TYSKRNIAQNLRGFRRLFAGCTATSQTSLGFDDWLRAVGAGDLSTRMLAQLELAEQSV
                     GGADPPLEQLLLNDPAAVRRIYDTVKSLTDLLKSEFVSVLELELPKSTEGDND"
     gene            2012..>2595
                     /locus_tag="VFQ35_15530"
     CDS             2012..>2595
                     /locus_tag="VFQ35_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9932114.1"
                     /translation="MTEGLRRLSARLLEPVDIAWLAAFRVMFGITLCVSMLRFLAYGW
                     VDELFVKPRLHFTYWGLGWVRPLSASHMHALFWVLAVLALCVAAGLAYRVTLGLFALG
                     FGYLQAVDVSNYLNHYYLALLLAFLLWAAPAHRALSLDAVLGLTRRRDYVPRAWHWLF
                     RFQVGVVYTFAGVAKAHADWLFHAEPLSIWLGGR"
BASE COUNT          404 a          847 c          852 g          492 t
ORIGIN      
        1 gtcgtactcg ggcgttcagc gtccggtgct cttcaagttg ggaccggacg gacaggggcc
       61 gcgctgggtc ttcagcgagc ccgtcggcac ggccgccgaa gcttacggcg ccgcgttgca
      121 gtcgaacggc aagttcgtca ccgcgggata tggtcgaccc aacgccgccg ccaccaccag
      181 cgacttcgtg tcgattcgtc tgaacgcgac gggcacgctc gatacgagct acggagtgag
      241 cggcgcccgc tggctggacg tcggcggctt cggtgacaac gcgcgcacgg tcgtcgtgct
      301 cggcgacgat cgcgtgttgc tcgccggtgg tggccgtttg acagccgatg atacggacgg
      361 cgccgtcgct atcctgaccg cgagcggcgc tcccgatgcg acattctccc ctgcgggctg
      421 caagacctac gatttcggca ctcccggcga ttttctctgg gcgggagcgc tctcgcctga
      481 caagaagttc gtggcgctcg ttgggatcac cggcgtttcg tcgagttcga tgctcgacaa
      541 cgattcgacc ttcttgctcc tgccggtcga gtgatgaaaa ggctcgcgag acgtgcacgt
      601 gcggtagcgc taaggcactg gagtgttgct ttggccgcgc cgactctcct tggcgcgata
      661 ggaccaagtt gccggtcgcc cgtgccggac gagcacgtct acacgggtcg cgacacgaac
      721 ggtcgcggtc cgtcgacagc gagcggtggt tcgactcagt tcgcgaaggg tggcactggc
      781 ccatcacctg acgcgagcgg cggacggggc ggcgcgctcc catctcttcc tccgggtggg
      841 agcgcgccgt atccggatga gtccggcggc gctagcgccg gcgaaggcaa cgtcgcgcat
      901 ggcggctctc cacccgtcac cgaggaaccc gcgccatctt tcagcaaaca ggcgctgctc
      961 ggcgccatcg cgagttgcgc cgccgaacag atcgacgagt tcgcggcacg cgccgacgcg
     1021 ctcaccgagc gtgccacggc tcacgaacgc gcccctggcg aggagagtct cgccgccgtg
     1081 cgcgcggcgt ggcaaacggc catggactcg ttccaggtgc tcgaaacctt gcgattcggt
     1141 ccgctcgcga gggcgagcga agatccgaag gggcgcgact tgcgggacca gatgtacgcg
     1201 tggccgctcg gtggtcgctg caacatcgag acacaactcg cctcgcaggg ttatgagggc
     1261 gcgagcttcg gcgcgtcgct catcaacctt cgagggcttg gtgcgctcga gtacctgctg
     1321 ttttacacgg cgagcgacaa cgcctgcccc gcgtattcgc cgctcaacaa ggatgggact
     1381 tgggccgcgc tcgggaccga cgagctcgct cgccgcaagg ctcgctacgc ggcgatcgtc
     1441 gcggcagacg tcgctcggca cgcccacgac ctgtacgacg actggaagct cgacggcgac
     1501 tttcgcactt cgttcgtctc cggggcagcg tatccgagcg cacaagcagc cttgaatgcg
     1561 gcgagcaatg ccctgtttta tgtcgaagtc gaagtcaagg actcgaagct cggcattccg
     1621 ctggggctct ctccagactg caccagcgcg acctgcccgg aggctctcga gtcgcgctac
     1681 gcgacctact cgaagcgaaa catcgctcag aacttacgcg gctttcggcg cctattcgcc
     1741 ggatgcaccg cgacttcgca aacatcgctc ggcttcgacg attggctccg cgcggtgggc
     1801 gcaggtgacc tttcgacgcg catgctcgcc cagctcgagc tcgcggagca aagcgtcggc
     1861 ggcgctgacc cgcccctcga acagctgctc ctgaacgacc ctgcggccgt gcgccgcatc
     1921 tacgatacgg tgaagtcgct gacggaccta ttgaagagcg agttcgtcag cgtgctcgag
     1981 ctcgagctcc ccaagtccac ggagggtgac aatgactgaa gggctacgtc ggctctcggc
     2041 gcgactgctc gaacccgtcg acatcgcttg gctcgccgcg ttccgcgtga tgttcggaat
     2101 cacgctgtgc gtgagcatgt tgcgcttttt ggcttacggc tgggtcgatg agctcttcgt
     2161 caaaccgcgt cttcacttca cgtactgggg actcggctgg gtgcgaccgc tctcggcgtc
     2221 gcacatgcac gcgctgttct gggtgctagc cgtgctcgcg ctatgcgttg cggcagggct
     2281 cgcctaccgc gtgactcttg ggctatttgc gctcggcttc gggtaccttc aggcggtcga
     2341 tgtctcgaac tacctcaatc actactatct agcgctgctg ctcgcattcc tcctgtgggc
     2401 agcgccggcg catcgtgcct tgtcgctcga tgcggtgctc ggcctcacca gacgacgaga
     2461 ctacgttcca cgcgcctggc actggctgtt tcgattccaa gtgggggtgg tttatacgtt
     2521 cgcgggggtg gccaaagcgc acgccgactg gctgtttcac gccgagccgc tctcgatttg
     2581 gttgggcggg cggag
//
LOCUS       DASTKS010000748         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_342537, whole genome shotgun sequence.
ACCESSION   DASTKS010000748 DASTKS010000000
VERSION     DASTKS010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342537"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..>2810
                     /locus_tag="VFQ35_15535"
     CDS             201..>2810
                     /locus_tag="VFQ35_15535"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET9932115.1"
                     /translation="MPRRLRTAPSALFVAIALELASGKSVAQTASPPPTASARAGSSE
                     SNSGDATASPESRALGALVGRDIERIEVLYDPPVWKSPVTLGGVQVGQTFTPEVARRA
                     LDELADTGRFGELRAEVEPLGTGVLLRLRVVARRTLLSLRLSGVSDADELLRNAGLRV
                     GSEVTARELPDLVRRLEQGLSRRGFPYSHVSVEALETDDPMGIVLSFQATRGAPARVL
                     EERFFAWPDPNAQGLATLLATYAVRRGDRADADALDAASHALEERLKRAGFYDARVEH
                     ELEPIRGGARVDVKVYAGSLRELHILGNKSFDISEIQAALSLDDTTDRSAAALAQRVR
                     EFYQVRGFLDVSVETRETGKPDAPLRQLSLIIHEGDAVRVIARDFPCLAGERAPDEVG
                     SEIDSFLSELPGGTLVDAVDPAEVDSLFGPKQSTGARPKPLKLNPWSVYSLDVYERAV
                     EHLRDLFRSEGYLSASVGPVIPERRRCDPRSPSGTCVPIGPRVRPQIACRYDAIGLPK
                     DESDVSSTACVPDLAKGVRCEPEMVLSIPIKLGPRSFLQAVQIDGNLSLSDEELLSAS
                     ELKVGAPVSQIEMDRARRRLLDRYAEEGFAFAEVDANLEFSPDHRQAKVVFTVSERKR
                     VGVSRIVVSGAHLTSESLIRRRIALEVGQLYRRSLVRKTEERLATLGVFSSVSVGLED
                     PYVPASEKVVVVNVQEKVPQAVEGRLGFSTGEGFRAFLEYGHNNLAGQAIGFTLHVQG
                     GFLPTPLIFEADVRRKYEPLSLLERLERRNTATLDFPEIGLGPLFRLSVQGIDVRHNA
                     RDYALRKDAGIVTLFFTPQRGLSFQLGGSLEGNNATIFGSGSVSQYVRTHPGLSRVFR
                     VPESPTFVIAQRAG"
BASE COUNT          432 a          948 c          962 g          468 t
ORIGIN      
        1 cgaggatacg gcgatcacgc acgcgcgggg ccaaggcgaa tagggctcga tccgggcgaa
       61 tcccttcggt ctcggggttt ggggctgcct attcgaacgc ggctttcggc catgttgccg
      121 cgggaaatga ggagaggcct ctgggcccga acgatacgag cggggtgaaa cagttccgct
      181 tctcgactaa gaagcgagga atgccgcgcc gccttcgaac cgcgccctcg gcgctcttcg
      241 tggcgatcgc gctcgagctc gcgtccggca agtccgttgc gcagaccgcg tctccgccgc
      301 cgaccgcttc cgcccgggcg gggagctcgg aaagcaactc aggggacgcg acggcgagcc
      361 cggagtcgcg cgcgctcggc gcgctggtcg gacgagacat cgagcgcatc gaggtgctct
      421 acgacccgcc ggtctggaag agcccggtga cgctcggcgg cgtgcaagtc gggcagacct
      481 tcacccccga ggtcgcgcgc cgggcgctcg atgagctggc cgataccggc cgcttcggtg
      541 agctgcgggc cgaagtcgag ccgctcggca cgggggtgct cttgcggctg cgcgtggtgg
      601 cgcggcggac cctgctcagc ctgcgtttgt cgggagtgag cgatgccgac gagcttttgc
      661 gcaacgcggg ccttcgggtc ggcagcgagg tcaccgcgcg cgagctgccg gacctggtgc
      721 gcaggctcga gcagggcctt tcgcggcgcg gttttccgta ttcgcacgtc tccgtcgagg
      781 cgctcgagac cgacgatccg atgggcatcg tgctgtcgtt ccaagcaacg cgcggcgcgc
      841 ccgcgcgcgt gctggaggag cgctttttcg cctggccgga tccgaacgcg caggggctcg
      901 caacgctctt ggcgacgtac gccgtgcgcc gcggcgatcg agcggatgcc gacgcgctcg
      961 acgcggcgag ccacgcgctc gaagaacgct tgaagcgcgc cgggttttac gatgcacgcg
     1021 tagagcacga gctcgagcca atccgcggcg gagcgcgcgt ggacgtcaaa gtttacgcgg
     1081 gctccctgcg cgagctccac atcctcggca acaagagctt cgacatctcg gagatccaag
     1141 cggccctgtc gctcgacgac acgacggata ggtccgcggc cgcgctggcg cagcgagtgc
     1201 gcgaatttta ccaagtgcgg ggcttcctcg acgtctccgt cgaaacgcgc gaaacgggca
     1261 agcccgacgc gccgctgcga cagctttcgc tcatcatcca cgaaggcgac gcggtgcgcg
     1321 tcatcgcgcg cgacttcccc tgccttgcgg gcgagcgcgc gccggacgag gtcggcagcg
     1381 agatcgatag cttcctctcc gagctccccg gcggcacgct cgtcgacgcg gtcgatcccg
     1441 ccgaggtcga ctcgctcttc gggcccaagc agagcacggg cgcgcgcccg aagcctttga
     1501 agctgaaccc gtggtccgtc tacagcctcg acgtctacga gcgcgccgtc gagcacctgc
     1561 gcgatctgtt tcgctccgag ggctatctgt ccgccagcgt cggccccgtg ataccggagc
     1621 gtcgacgttg cgacccgcgc tcgccgtccg gcacctgcgt gcccatcggc ccccgcgttc
     1681 gcccccaaat cgcgtgtcgc tacgacgcca tcggtctgcc caaggacgag tccgatgtct
     1741 catcgaccgc ctgtgttccg gatctcgcga agggcgtgcg ctgcgagccc gaaatggtgc
     1801 tcagcattcc gatcaagctc gggccgcgca gcttcctaca ggccgttcaa atcgacggga
     1861 acctgagcct ctccgacgaa gagctgctct cggcctccga gctcaaggtg ggggcaccgg
     1921 tctcgcagat cgagatggat cgcgcgcgcc gccgtctctt ggatcgctac gccgaagagg
     1981 gctttgcctt cgcggaggtg gacgcgaacc tcgagttttc gcccgaccat cgccaggcca
     2041 aggtcgtgtt caccgtgagc gagcgcaagc gcgtcggtgt ctcgcgcatc gtggtgagcg
     2101 gcgcgcacct cacgagcgag agcttgattc gacgtcgcat cgcgctcgag gtcgggcagc
     2161 tctaccggcg tagcctggtg cgcaagaccg aagagcggct cgcgacgctg ggcgtcttct
     2221 cgtcggtgtc ggtggggctc gaagatccgt acgttcccgc gagcgagaag gtcgtcgtcg
     2281 tcaacgtgca agagaaggtg ccccaagcgg tcgagggacg gctcggcttc agcaccggcg
     2341 aggggtttcg cgccttcttg gagtacgggc acaacaactt ggcggggcag gccatcggct
     2401 tcacgctgca cgtgcagggc ggcttcttgc cgactccgct catcttcgaa gcagacgtga
     2461 ggcgcaagta cgagccgctc agcttgctcg agcggctcga gcgcaggaac accgcgaccc
     2521 tcgactttcc ggagatcggc ttgggtccgc tgttccgcct gagcgtgcag ggcatcgacg
     2581 ttcgccacaa cgcgcgcgat tacgcgctcc gcaaggacgc cggcatcgtc acgctgttct
     2641 tcactccgca gcgcggtctg tcgtttcaac tcggcggctc gctcgagggt aacaacgcga
     2701 ccattttcgg cagcggttcg gttagccaat acgtgcgcac gcacccgggc ctgagccgag
     2761 tgttccgtgt gcccgagagc cccaccttcg tcatcgcgca gcgcgcgggt
//
LOCUS       DASTKS010000749         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_342625, whole genome shotgun sequence.
ACCESSION   DASTKS010000749 DASTKS010000000
VERSION     DASTKS010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342625"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFQ35_15540"
     CDS             complement(<1..900)
                     /locus_tag="VFQ35_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HET9932116.1"
                     /translation="MGVTALRSEVALTRLREFLHGDLGAVLERSQRCDPSAEALKLFH
                     DVAANVPAYAKFLAARGVDPRAVQTAEDFARLPSTSKAEYHKAYPLAELCRDGNLSAC
                     DFLSVSSGSTGEPTLWPRFASDEYGTARRFEQVLLDGFEASSKRTLGVVCFSLGSWVG
                     GMYTTFACRSLALKGYPLTLVTPGNNRAEILRVVRALAPSFEQVVLFGYPPFLKDVID
                     AGRADGLDWSRVPTRLVMAGEVFSEEWRALVCERLGGVDPALHTASLYGTADGGVLAN
                     ESPVSVRIRRLLAEHPAAARELFG"
     gene            complement(903..2672)
                     /gene="thiC"
                     /locus_tag="VFQ35_15545"
     CDS             complement(903..2672)
                     /gene="thiC"
                     /locus_tag="VFQ35_15545"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET9932117.1"
                     /translation="MIGNLPLRGAHNPSSRQQGTNPGSFALPPNIGGPLTYASPDTPG
                     MPAPSEKTAWDFLPDGWRREGAHAVAPEGFVPVTQLEFARLGIVTEQMRRVAEREPHL
                     TPEAVRDEVAAGRMIIPANKVHLGYALEPMCIGRASRTKVNANMGASPVSSSTDEEVE
                     KLKWAERWAADTVMDLSTGGDLDACREAIIQNARVPIGTVPIYSMIIGRSIEELGEED
                     ILEGLRHQAKQGVDYFTIHAGVLREHLPFVKKRLIGIVSRGGSLLAKWMITHGKQNPM
                     YTLFDQICDIMREYDVSFSLGDGLRPGGLADASDDAQLRELETLGELTERAWRRGCQV
                     MIEGPGHMPFDQIEYNMKLQRTLCHGAPFYVLGPLVTDVFPGYDHITSCIGATAAAYH
                     GASMLCYVTPKEHVGLPKKDDVKQGCVAYKIAAHAADVALGIPGSRDWDDELTKARAA
                     LNWEKHFELAFDADTARAFHDEDLDVDTDFCAMCGHDWCSVRISKEIVEFASGKHEGF
                     ERDGVQKSKAINVEQRAILEKRGVLSPEEIERLASKTARAVGASRGHKAGCHSDTTDA
                     NSARDLQQQLVELRKKPAADAVS"
     regulatory      complement(2711..2805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3062..4402)
                     /locus_tag="VFQ35_15550"
     CDS             complement(3062..4402)
                     /locus_tag="VFQ35_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932118.1"
                     /translation="MASEHQDENPEPVYVIESIPVGGEHEVTPADVLREGIPVPSHLR
                     VFEELGRGAMGRIHPATDRNLLRHVALKRLDKQLAKEPFYRDGFVAEAQITGQLEHPN
                     IVPVHELSIDKSGVPYFTMKLVQGENFGRWLRNPDRAPGMHERLEEGLDILSKVCDAV
                     AYAHHRGVIHRDLKPENIMVGAFGQVYLMDWGLARLTKTRPASGDYAQMEAPGPVGTP
                     EYMAPEQARGNPSDMDERSDVFGLGAVLYEIVSGKRPYGEAKSQEIVLGRAKIGAVVP
                     IDEATMGLGIPKRIRSIVEKALAPEPKDRYQTVLELKRDIHEFLRGGLHLPRRNFPSG
                     SVIIREGDAGDAAYLIVSGTCRAYRDVAGQKEVLATMNAGDVFGEMALLLDELRAASV
                     EAVTPVTVLVLDRATISEGLGVNGWTGALVHALAQRFRALEQTIRNSGIVRRET"
     gene            complement(4644..>5592)
                     /locus_tag="VFQ35_15555"
     CDS             complement(4644..>5592)
                     /locus_tag="VFQ35_15555"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932119.1"
                     /translation="EDTITRREAVRDLLVRSGLQETIAYSFNGPELESKIFRGHPGAE
                     PPRPVVVLNPVSVDRSVLRTSLLPALLAAAANNLRHEASCRLFEIGPVFTPGADVRDL
                     PVEEERVSVVIGGLARVPSLHEPKPPRLDFFDAKGLIGEVLASLRVADGVEWVPHAEA
                     PYRPGAAARIVRGGRTYGTVGAVHPLVLRAFDMEEQSVFVADLSLKALLQDSPRRPTF
                     HEYDRLPSIELDVAMFVGVATPAADIRKVAERAAGPLLRSVEVFDQFFNPELPEQKSI
                     AIRLRLNAGERTLEMPEALSVRERVARALEAELSAKIRE"
BASE COUNT          959 a         1871 c         1812 g          950 t
ORIGIN      
        1 accaaagagc tctcgcgccg ccgcgggatg ctccgcgagc aagcgccgaa tgcgtacgga
       61 cacggggctc tcgttggcga gaactccgcc gtcggcagtc ccgtacaggg acgcggtgtg
      121 gagcgcgggg tcgacacccc cgagtcgttc gcaaacgagg gcgcgccact cttcgctgaa
      181 cacctcgccg gccatgacga gccgagtggg cactcgcgac caatcgagcc cgtccgccct
      241 acccgcgtcg atcacgtcct tcagaaacgg cggataaccg aacaaaacga cctgctcgaa
      301 ggagggggcg agcgcccgca cgacgcgcaa gatttccgct cgattgttgc ccggcgtgac
      361 gagcgtgagg gggtatccct tcaaagccag cgagcgacac gcgaaggtcg tgtacatgcc
      421 gcccacccag ctcccgagtg agaagcaaac gacgccgagt gtccgcttcg acgacgcttc
      481 gaaaccgtcg agcaacacct gctcgaagcg gcgcgccgta ccatattcgt cactcgcgaa
      541 ccgcggccag agcgtcggtt cgcccgtcga gcccgaagac acggacagga agtcacaggc
      601 gctcaggttt ccatcgcggc agagctcggc gagcgggtac gccttgtggt actccgcttt
      661 gctcgtggac ggcagacgcg cgaagtcttc cgcggtctgg acggcgcgcg gatccacgcc
      721 gcgcgcggcc aagaacttcg cgtaggcagg gacgttcgcc gcgacgtcgt gaaagagctt
      781 gagggcctcc gcgctcgggt cgcagcgctg cgagcgctcg agcaccgcgc cgagatcgcc
      841 gtgcaagaac tcgcgaagcc gcgtgagagc gacttcgctg cgaagagcgg tgacccccat
      901 ggtcagctca ccgcgtcggc ggccggcttc tttcgcagct cgaccagctg ctgctgcagg
      961 tctcgcgccg agttcgcgtc ggtcgtatcg ctgtggcagc ccgccttgtg accgcgcgaa
     1021 gcgccgaccg cgcgagcagt cttcgaggcg agccgctcga tctcttccgg cgacagcaca
     1081 ccgcgcttct ccagaatcgc gcgctgctcg acgttgatgg ccttcgactt ctgcacgccg
     1141 tcgcgctcga agccttcgtg cttgccgctc gcaaactcga cgatctcttt cgaaatccgg
     1201 accgagcacc agtcgtgccc gcacatggcg caaaagtcgg tgtccacgtc gaggtcttcg
     1261 tcgtggaaag cgcgcgcggt gtcggcgtcg aaggcgagct cgaaatgctt ttcccaattg
     1321 agcgcggcgc gagccttcgt gagctcgtcg tcccaatcgc gcgaaccggg gatcccgagc
     1381 gcgacgtccg cagcgtgggc cgcgatcttg taggcgacgc agccctgctt cacgtcgtcc
     1441 tttttcggca ggcccacgtg ctctttggga gtgacgtaac agagcatcga agcgccgtga
     1501 taggcggccg cggtcgcgcc gatgcagctc gtgatgtggt cgtagccggg gaacacgtcg
     1561 gttacgagcg ggccgagcac gtagaagggc gcgccgtggc acagcgtgcg ctgcagcttc
     1621 atgttgtact cgatctgatc gaagggcatg tgaccggggc cctcgatcat cacctggcag
     1681 ccgcgtcgcc acgcgcgctc ggtgagctca cccagggttt cgagctcgcg tagctgcgcg
     1741 tcgtccgaag cgtcggcgag accacccggg cgcaggccgt cacccagcga gaacgacacg
     1801 tcgtactccc gcatgatgtc gcagatctgg tcgaacagcg tgtacatcgg gttctgcttg
     1861 ccgtgggtga tcatccactt ggcgagcagc gagccaccgc ggctcacgat gccgatcagg
     1921 cgctttttga cgaaagggag gtgttcgcgg aggacaccgg catggatggt gaaatagtcg
     1981 acgccctgct tcgcctgatg ccgaaggccc tcgaggatgt cttcttcgcc gagctcttcg
     2041 atgctgcgac cgatgatcat cgagtagatc ggcacggtgc cgattggcac gcgcgcgttt
     2101 tggatgatcg cttcacggca ggcatccaaa tcgccgccag tcgaaaggtc catcaccgtg
     2161 tcggccgccc agcgctccgc ccacttcagc ttctcgacct cttcgtcggt gctcgacgaa
     2221 actggcgacg cgcccatgtt cgcgttcacc ttggtacggc tggcgcgccc gatgcacatc
     2281 ggctcgagcg cgtagccgag gtgcaccttg ttggcgggga tgatcatgcg cccggccgcc
     2341 acctcgtcgc gcaccgcttc gggggtgagg tgcggctcgc gctcggcgac gcgacgcatt
     2401 tgctcggtca cgatcccgag gcgagcaaac tcgagctgcg tcacgggaac gaagccctct
     2461 ggcgccaccg cgtgtgcacc ttcgcgacgc cagccatcgg gcaagaagtc ccacgccgtc
     2521 ttctcactcg gcgccggcat gccgggcgtg tcgggcgatg cgtacgtgag cgggccgcca
     2581 atgttcggcg ggagggcgaa cgagccgggg ttcgtcccct gctgccggct ggaaggattg
     2641 tgggctccgc gaagtggcag gtttccaatc atgggcgtcc tcgtcttttc gggggaaagg
     2701 cgacgccgat cggaatcgtg cgacgctttc cctacgccgg tgcgaaccgg atcaggttct
     2761 acgggtgctt ctcagcggca atgggccgcg ccccgagcga cggtcggacg ctagctcatg
     2821 gcccattggc tcgcaagccc gacctttcgc ggtccggcac ggaaatgcgc gcatcgcgag
     2881 tccgtcgagc gcgcagccgg aagcctggac caactgcggg gctcgtgcga gcgccgcgac
     2941 gacacgcgta gcgcaagagc tcgggcgact cacccgtttg cgcaccgagg ttccgtcgga
     3001 cgagcctcga gccgtgcaga gaagagcgag cgttctgaaa agccgccgaa actcgagaag
     3061 gtcacgtttc gcggcgcacg atgcccgaat tgcggatggt ttgctcgagc gcgcgaaaac
     3121 gctgtgccaa cgcatgcact agggcaccag tccagccgtt cacaccgagg ccctcgctga
     3181 tcgtcgctcg gtcgagcacc agcacggtga cgggggttac cgcctcgacg ctcgcggcgc
     3241 gtaactcgtc gagcaagagc gccatctccc caaaaacgtc ccccgcgttc atggtcgcaa
     3301 gcacttcctt ttggcccgcg acgtcgcgat aagcgcggca cgtgccgctc acgatgaggt
     3361 aagccgcatc gcccgcgtcg ccttcccgga tgatgacgct gccggacggg aagttgcgac
     3421 gtggcaagtg cagccctccc cgcaagaact cgtggatgtc acgcttcagc tcgagcaccg
     3481 tctggtaacg atctttcggc tccggtgcga gcgccttctc gacgatgctg cgaatccgct
     3541 tcgggatgcc gagccccatc gtcgcctcgt cgatcggcac gaccgcgccg attttggcgc
     3601 gcccgagcac gatttcttgc gacttggctt cgccgtaagg ccgcttaccg ctcacgattt
     3661 cgtagagcac ggcgccgaga ccgaacacgt ccgagcgttc gtccatgtcg ctcggattgc
     3721 cacgcgcctg ctcgggggcc atgtactcgg gtgtcccgac gggaccgggc gcctccatct
     3781 gcgcgtagtc gccagaagcc ggccgcgtct tggtgagccg cgcgagcccc caatccatca
     3841 ggtagacctg gccgaacgcg cccaccatga tgttctctgg cttcaggtcg cgatgaatga
     3901 cgccgcggtg atgcgcgtac gcgaccgcgt cgcaaacctt cgacaagatg tcgagccctt
     3961 cctcgagtcg ctcgtgcatt ccaggcgccc gatccggatt tcgcagccaa cgcccgaagt
     4021 tctccccctg cacgagcttc atcgtgaagt acgggacgcc gctcttgtcg atcgagagct
     4081 cgtgaacggg aacgatgttc gggtgctcga gctgccccgt gatctgcgcc tcggcgacga
     4141 agccgtctcg atagaagggc tctttcgcga gctgcttgtc gaggcgcttc aacgccacgt
     4201 gccgaagcag gttgcgatcc gtcgcgggat ggattcgccc catcgcgccg cgcccgagct
     4261 cttcgaagac ccgaaggtgc gaagggaccg ggatgccttc acgcaggacg tctgcgggag
     4321 tcacttcgtg ctctcccccg accgggatcg actcgatgac atagacgggc tcggggttct
     4381 cgtcttggtg ctcggaagcc acgcgcaaaa cctgctttca gactggagac gctcgagaag
     4441 ccccagaggc ttgccgtcgc ccggcagcag atcgcttcgc caagctcggt gtaggatctg
     4501 ggacttttct taaacgcgcg gagagtagcc tgttttcccg cgtagcatcg cggttttggc
     4561 gcaagaacct gccaactacg ccgaaaacct agagatcttt cggggttacg gcagcgccga
     4621 ccggacgtcg ccggaacccc tgtctactcg cgaatcttcg ccgaaagctc ggcctcgaga
     4681 gcccgcgcga cgcgctcgcg aaccgagagc gcctccggca tttcgagcgt gcgctccccc
     4741 gcgttcaagc gaaggcggat cgcgatcgac ttctgctcgg gtagctcggg gttgaagaac
     4801 tggtcgaata cctcgacgct tcgaaggaga ggccccgccg ctcgctccgc cactttgcga
     4861 atgtcggccg caggcgtcgc gacgccgacg aacatggcaa cgtcgagctc gatcgagggc
     4921 agccggtcgt attcatggaa cgtcggcctg cgcggcgagt cttggagcag cgccttcaag
     4981 gacaggtccg cgacgaacac cgactgctct tccatgtcga acgctcgcaa cacgagcggg
     5041 tgcaccgcgc cgacggtccc gtacgtgcga ccgccacgca cgatgcgcgc cgcggctccc
     5101 gggcgatacg gcgcctctgc gtgcggcacc cattcgacac cgtcagcgac gcgaaggctc
     5161 gcgagcacct cgccgatgag ccccttcgcg tcgaagaagt ccaagcgcgg cggcttcggc
     5221 tcgtgcagcg acggcacccg cgcaagccca ccgatgacga cgctcacgcg ctcttcttcg
     5281 acgggcaggt cccgcacgtc ggcgcccggc gtgaacacgg gaccgatctc gaagaggcgg
     5341 catgaggctt cgtggcgcag gttgttcgcg gcggccgcga gcaaggccgg caagagcgac
     5401 gtgcgcagca ccgaacgatc gacgctcacc gggttcaaga cgacgaccgg gcgcggcggc
     5461 tcggcccctg gatgcccgcg gaaaatcttg gactcgagct ccgggccgtt gaacgaatag
     5521 gcgatcgtct cttgcaggcc cgagcgcacc aggagatcac gaacggcttc gcgccgagtg
     5581 atcgtatcct cg
//
LOCUS       DASTKS010000750         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_120294, whole genome shotgun sequence.
ACCESSION   DASTKS010000750 DASTKS010000000
VERSION     DASTKS010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120294"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFQ35_15560"
     CDS             complement(<1..204)
                     /locus_tag="VFQ35_15560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9932120.1"
                     /translation="MSITTRRNFLRTFAAAAGVGIGSNLLPPAIRKALAIAPRRCKGT
                     IEDVEHVVILMQENRSFDHYFGTL"
     gene            908..>2143
                     /locus_tag="VFQ35_15565"
     CDS             908..>2143
                     /locus_tag="VFQ35_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932121.1"
                     /translation="MFEAVTFENASRLPLRALLLASVALAGCHGKRAAPTPSEPSSAS
                     AQASHAVVPAAKFDRVSRGDFNRLASELNLPLFWSEDKNRNGALEPEELAVYWGLAPG
                     ARLTDYVVAERNAFTARMTNAYDAIVRRQAGIVVNPGDPKEAARRAAVVKELAQGRPT
                     LVATDFSKASEEERAFVALVMKSAELVEQIYAKQLGTAALKLKVPADDTASQTLFFRS
                     QGPRCQAPLTQDDPLCSAIPDFTEKKLSGLYPHDLLNQPNFCEALAKRDKALMDPFVA
                     VAGDVNAPRALPYNQAYAPEMGQIAEQLRAAARAIGDKEPALQNYLTAAAQSFVKNAW
                     WPADEAWAKMDAKNSKFYLRIGPDETYEEPCNSKALFHVSFGLINQGSLAWQQKLEPF
                     RSDMESALAELAGPPYKARK"
BASE COUNT          431 a          691 c          671 g          350 t
ORIGIN      
        1 gagcgtgccg aagtagtggt cgaaggaacg attttcctgc atcaggatga cgacgtgctc
       61 cacgtcttcg atggtgcctt tacagcgccg cggcgcaatc gcgagcgcct ttcgaatcgc
      121 ggggggcaag aggttcgagc cgatgccgac gcccgccgca gccgcgaacg tgcgcaagaa
      181 gttacgtcga gtcgtgatgc tcatgaggct tcctttcgaa cgcgaggaga gacacggggc
      241 acgaacgccc tcacgtgctc ggctcgacgc tcacacgact cggtggcgct gacgtgacgt
      301 tgcctcagcg attgcgcagt gactcgccga cggctgcgcg caccgagccg gcgctcggcg
      361 ccttcgagct gctcttcagg caaggcagca gacggagctg aggatcgctg accgcgggtc
      421 acccgctccg cgaggcttga accctactga gctgaggagc agaagtcgcg gcgcgctgga
      481 cattcttgcg caccgacaga ggattcgaac accctggacg attatcacgc agggctcctc
      541 gattcgcact gaggagcacc caatgcggca cccgacgaac gatgtttcga tgattgtcac
      601 caaagtttcg ccaaggcgta cgtgcgatgc cccggtgcat ttgcgagcgg cgacgcgcgc
      661 caaaacgcct cgagccactt cggccggtgc atcgagccgc tcgccgtcgg cgccgagtgc
      721 aagaaggagc tctacggggc gtgtaacggc tacacctgca acacgaccgc cgatcccggt
      781 gaggccgggt cgacgtgtac cggtggaaaa tgcacatcga acaggggcaa taccctctcg
      841 tcggagaacg atccggggcg ttcctttcgc gccacgtcgc gctcgtctgt ggtagacgtc
      901 gaagccaatg ttcgaagcgg tgacgttcga aaacgcctcg cggcttccac ttcgggcgct
      961 cttgctggcc agcgtggctc tcgccggttg ccacggtaaa cgcgccgcgc cgactccgtc
     1021 agagccatcg agcgcgagtg cgcaggcgtc gcacgcggtt gtgcccgccg cgaagttcga
     1081 tcgagtctcg cgcggcgact tcaaccggct cgcgagcgag ctcaacttgc cgctcttctg
     1141 gtccgaggac aaaaatcgga acggcgcgct cgagcccgaa gaactcgccg tgtattgggg
     1201 actcgccccg ggagctcggc tcaccgacta cgtcgtcgcg gagcgcaacg cattcacggc
     1261 aagaatgacg aacgcgtacg acgccatcgt acggcgccaa gccggcatcg tcgtcaaccc
     1321 aggagatccg aaggaagccg cacgccgcgc cgccgtggtg aaagagctgg cacaggggcg
     1381 tccgaccttg gtcgccaccg atttttcgaa agcgagcgag gaagagcgcg cgttcgtcgc
     1441 gctcgtgatg aagtcggcgg agctcgttga gcaaatctac gcgaagcagc tcgggacggc
     1501 agcgctcaag ctcaaggttc ccgccgatga tacggcctcg cagactctgt tttttcggag
     1561 ccagggccct cgctgccaag ctcctctgac acaagacgat ccgctctgca gcgccatccc
     1621 cgacttcacc gagaagaagc tttcgggtct ctatcctcac gacctactca atcagccgaa
     1681 cttctgcgaa gcgttggcca agagagacaa ggccttgatg gacccattcg tcgcggtcgc
     1741 aggcgacgtg aacgcgcccc gcgcgctgcc ctacaaccaa gcctacgcgc cggagatggg
     1801 gcaaatcgcc gagcaactaa gggccgccgc gcgagccatc ggcgataaag agcccgccct
     1861 ccagaactat ttgacggccg ccgcccagtc gttcgtgaaa aatgcgtggt ggcccgccga
     1921 cgaggcctgg gcgaagatgg acgcgaagaa ctccaagttc tatcttcgca tcggcccgga
     1981 cgagacctac gaagagccct gcaactccaa agctctgttt cacgtgagct tcggcctcat
     2041 caaccaaggc tcgctcgcat ggcagcagaa gctcgaacct ttccggagcg acatggaaag
     2101 cgcgttggca gagctcgcgg ggccgcccta caaagcgagg aag
//
LOCUS       DASTKS010000751         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_566285, whole genome shotgun sequence.
ACCESSION   DASTKS010000751 DASTKS010000000
VERSION     DASTKS010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566285"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..2807
                     /locus_tag="VFQ35_15570"
     CDS             573..2807
                     /locus_tag="VFQ35_15570"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HET9932122.1"
                     /translation="MSRLVVISNRLPFSIESQGDEVRFAPSAGGLVSALGAYLERRRA
                     DEPDFQCVWVGWPGAAVPVEAEERVRRTALERHSAFPIFLTTDEVHSFYHGFCNSTLW
                     PLFHYFPSYTVYDPREWETYERVNRRFCDAVLELLRPDDTIWVHDYQLFLLPGMLRAA
                     LPEAIIGFFLHIPFPSHELFRLLPSRWQRELLSGMLGADLVGFHTFDYTQHFLQCVYR
                     TLGHDHDLGQIAFGDQARRADTFPIGIDFERFMSAATSPRVAEIRAEIERGLEGRTAI
                     FSVDRLDYTKGILNRLRAYEEFLKTRPEWLGKVVLLLTVVPSRAEVPQYRRMKQAIDE
                     RVGRINGRFGSTGWVPIIYQYRSVDFETLVALYQLSPIALITPLRDGMNLVAKEYLGS
                     KPDGRGVLILSEMAGAARELGEAVIVNPNDENEVVGALESALAMPEDEQVRRNRAMQE
                     RLKNYDATRWATHFLSTLANVKATQGSLATRSLKGHAASEVQARYAKSSKRLVFLDYD
                     GTLVPFAAEPRAAVPDKKLLAILGALCDDTANEVYLISGRDRSTLTQWFEGIGLNFVA
                     EHGAWLRQRDLEWRLLKPLTSEWKERLVPILKTYADHLAGALLEEKEYSLAFHYRRCD
                     PELGAQRAKQLIYELRRFTANFNVQVLEGKKVVEVRNAGVTKGSAAAQIASELNPDFI
                     LAIGDDETDEDLFRSLPAGAVSIRVGTPFSYAQFSLSGHREVRALLVSLIESSRGPTS
                     FREG"
     gene            complement(2816..3316)
                     /locus_tag="VFQ35_15575"
     CDS             complement(2816..3316)
                     /locus_tag="VFQ35_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932123.1"
                     /translation="MGRARLGMMNRWWPLFATACLVCCGPDSKPRQQCAGKPDFVVTV
                     SAPPGLLPEDTTVLVTFGGDGHESYRPNAHNERQVLFCEPTRMPGGGGEGGSTSADDQ
                     ELAGAGGASNPVATISAITCELWTGGPASIRVRAGELEVEQTLTPKAETCTVWTSLVL
                     GRDKEP"
     gene            3511..4446
                     /locus_tag="VFQ35_15580"
     CDS             3511..4446
                     /locus_tag="VFQ35_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9932124.1"
                     /translation="MSFDQIRHRLSSGRPLVLDADSGASFRARGVELDMPGTLGVLLR
                     EQPREVLSHHLAEVASRVDVLSALTVDTTPRALAEVGMQHRSAQLTGRALDLALEAAS
                     DAMRAVAVAGVLGSDMVGPIAADRLQEELAEHASRLKVSGAELIIARGQGSRMSLMAA
                     VSTAVELELPTWAVLECSGDSELESSSLGEFFESLRDAGTSAVLLEVPSVRVGLAELE
                     RSRPALDALGLVAGVLLAASDKAVRGFPDPTSEPEQWAEHAIDLSLAGARVLGGGAGT
                     TESHTRALALALGALHPSVPPPRAASEIDRRPRDW"
     gene            4459..>5228
                     /locus_tag="VFQ35_15585"
     CDS             4459..>5228
                     /locus_tag="VFQ35_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET9932125.1"
                     /translation="MQRQSLDDNAPLGDTDALTSVFHAAEKPVSAFRLGAEGEKFGVH
                     ELTGAPLSYEGEHGVARVLAALTRFGWQPESETEGGPIIALTRGAASVTLEPGSQLEL
                     SGSALPDVHAVQAEFAEHMQELADISREMQLAWLGVGFHPIAQQAELTWVPKQRYGIM
                     REYLPPLGSAAHDMMRRTATVQVNMDYASEEDAMRKLRIGLLLAPLLNAMTANSPFRE
                     RARTDKKSLRGNVWLHMDPSRSGLVRRVLAKKRATYRD"
BASE COUNT          866 a         1690 c         1746 g          926 t
ORIGIN      
        1 ccgaagccga gacgaacgaa ggcaacttga gcgagtcgtt ggtgctgcgc tcgcacgggc
       61 cgcgcgtcga tctctcggct ccgaaccagt gactgtccgt catccgtcgc tacgcgcttt
      121 ccaccaccga cacgggcttg cgcgcctcat cgaggcgcgg acgcgaccag cacggaacgc
      181 cgctcggtcg ttacggccgc ttggcggcag atgggcgagg cggccgtacg cgaacccagc
      241 attgagctac gcgaggtggg gcgcgaacgt cccatgattt ctacgctcga gcccccgcgt
      301 ggttgaatcg gcgcgttccg ggtggcgaaa gagagctgcg aggccgacga agccagcgtg
      361 gcgaacgtgc agcgcgcgcg cctcactagc tattggtggg gcaccccgat agacttctgt
      421 ttttgggtgt cgcggcaacg gggctgcgac aggttacctc ggaaggtcgg gccgcctccg
      481 aacgaacggc gctagttgca cgcaccttgg catgccgcca cacacggtcg tcctcgggac
      541 gtgtcgcagc gctctttccc agcagggtca acatgtctcg tttggtcgtc atttcgaatc
      601 ggctgccgtt cagcatcgag agccaaggcg acgaagttcg cttcgccccg agtgccggtg
      661 gattggtcag tgccctcggc gcgtatctcg agcgccgtcg cgcggatgag ccggatttcc
      721 agtgtgtgtg ggtgggatgg ccgggcgcgg cggtgccagt agaggccgaa gagcgtgttc
      781 gccgcacggc gctggaaagg cattcggcct tcccgatctt cctgacgacc gacgaagtgc
      841 actcgtttta tcacggcttc tgcaacagca cgctgtggcc gctgtttcac tacttccctt
      901 cgtacacggt ttacgatccg agagagtggg agacctacga gcgggtgaat cgacgcttct
      961 gcgatgcggt gctcgagctc ttgcgaccgg acgacaccat ctgggtgcac gactatcagc
     1021 tctttctcct gcccgggatg ctgcgagccg cgctccccga ggcgatcatc ggattctttc
     1081 tccacatacc gttcccctcc cacgagctct ttaggctgct gccgtcgcgg tggcagcgcg
     1141 agctcttgtc ggggatgttg ggcgcggact tggtgggctt tcacacgttc gattacacgc
     1201 agcacttctt gcaatgcgtg tatcgaaccc tcggtcacga ccacgacctc gggcagatcg
     1261 cgttcggcga ccaggcgcgc cgggccgaca cctttccgat tggcatcgat ttcgagcgtt
     1321 tcatgagcgc ggcgacgagc ccgcgcgtcg cggaaatccg cgctgaaatc gagcgaggtc
     1381 tcgaggggcg caccgccatc ttttcggtgg atcggctcga ctacacgaaa ggcatcctga
     1441 accgcctgcg cgcttacgaa gagttcctga agactcgtcc ggagtggctc ggtaaagtcg
     1501 tcttgctgtt gacggtcgtg ccgtcgcgcg cagaggtgcc gcaataccgg cgcatgaagc
     1561 aagccatcga cgagcgggtc ggccgcatca atgggcgctt cgggtcgacg ggctgggtgc
     1621 ccatcatcta tcagtaccgc tcggtcgact tcgaaacgct ggtcgcgctc taccagctct
     1681 caccgatcgc gttgatcacc cccctgcgcg acggcatgaa tctcgtcgcg aaggagtatc
     1741 tcggctcgaa accggacggg cgcggcgtgc tcattttgag cgagatggcg ggcgcggcgc
     1801 gcgagctcgg cgaagccgtg atcgtgaacc cgaacgacga aaacgaggtc gtcggcgcgc
     1861 tcgagagcgc cctcgccatg cccgaagacg agcaggtgcg caggaatcgc gcgatgcagg
     1921 agcggctcaa gaactacgac gcgactcgct gggcgacgca ctttctgtcg acgctcgcga
     1981 acgtcaaggc gacgcaaggc tcgctcgcga cccgctcact gaagggtcac gctgcgagcg
     2041 aggtgcaggc gcgctacgcc aaatccagca agcgtctggt cttcctcgac tacgacggca
     2101 cgttggtgcc gttcgcggcg gagcctcgcg cggccgttcc cgacaagaag ctgctcgcca
     2161 tcctcggcgc gctctgcgac gacaccgcca acgaggtgta cttgatcagc gggcgcgacc
     2221 gctcgacatt gacgcagtgg ttcgagggca tcggcttgaa cttcgtggcg gagcacggcg
     2281 cgtggttgcg tcagcgcgac ctcgagtggc ggctattgaa gccactcacc tcggagtgga
     2341 aggaacgcct ggtgccgatc ctgaaaacgt acgcggacca cctcgcgggc gcgctgctcg
     2401 aggagaagga gtattcgctc gcctttcact atcgtcgctg cgacccggag ctcggcgccc
     2461 aacgcgcgaa gcaactgatt tacgagctcc ggcggttcac cgcgaacttc aacgtgcagg
     2521 tgctcgaagg caagaaggtc gtggaggtgc gcaacgccgg cgtcaccaag ggctcggcgg
     2581 ccgcacaaat cgcctccgag ttgaacccag atttcatctt ggccatcgga gacgacgaga
     2641 ctgacgagga tctgttccgc tccctgccgg ccggggcggt atcgatccgc gttggcacgc
     2701 cattctcgta cgcgcagttc agcctgagcg gtcaccgcga agttcgggcg ctgctcgttt
     2761 cgctcatcga gagcagccga gggccaacga gcttccgcga aggttagagg cgcgctcacg
     2821 gctccttgtc tcgtcccagt acgagcgagg tccagaccgt acaggtctcg gctttgggcg
     2881 tgagcgtctg ctccacctcg agttcgccgg ctcgtacgcg gatcgacgcg ggcccgcccg
     2941 tccagagctc acacgtaatg gcgctgatcg tggcgacggg gttgctagcg cctcctgcgc
     3001 ccgcgagttc ctggtcgtcg gcgctcgtcg agccgccctc cccacctccg ccaggcattc
     3061 gagtcggctc acagaataga acctggcgct cgttgtgcgc gttcgggcgg tagctctcgt
     3121 ggccatcgcc cccgaaggtc acgagcacgg tcgtgtcctc gggtagcagc ccgggagggg
     3181 cgctcacggt gacgacgaag tcgggtttcc cggcgcactg ttgccgaggc ttcgaatcgg
     3241 gaccgcagca aacgaggcaa gcggtcgcaa aaagcggcca ccagcggttc atcattccga
     3301 ggcgcgcgcg acccatggcc cgctgcgcat aaggcagagc ccaccgttca gcaacacctg
     3361 aatgcgaacg gttcggttct ttcggctcga agcccacatc ggaggccgag ggagcaggct
     3421 ctgcgcgcgt gtgcggccga tcgctcgtcg tcgctttgtt ctgtctacgc gatgccgttc
     3481 gttccgtggg atcatcgatt ggcccatcgc atgagcttcg accagattcg gcatcgcctg
     3541 agcagtggac gtcctttggt tctcgacgcg gacagcggcg cgtcgtttcg cgcgcgcggc
     3601 gtggaactcg acatgccggg aactctcggc gtgctcttgc gtgagcagcc gcgagaagtg
     3661 ctgagccacc atttggcgga agtcgccagc cgcgtcgacg tgctctccgc gctcacggtc
     3721 gacacgaccc cgcgcgcgct cgcggaggtg ggcatgcaac atcgctccgc tcagctcaca
     3781 ggtcgtgcgc tcgatttggc gctcgaggca gcgagcgacg cgatgcgggc ggtggcggta
     3841 gcgggagtgc tcggttcgga catggtcggc ccgatcgccg cggatcgctt gcaggaagag
     3901 ctcgccgagc acgcgtcgcg cttgaaggtc tccggcgccg aactcatcat cgcgcgtggc
     3961 cagggctcgc gcatgagcct gatggcagcc gtgagcaccg ccgtcgagct cgagctgccg
     4021 acgtgggcgg tgctcgagtg ctcgggtgac agtgagctcg agagctcatc gctcggcgaa
     4081 ttcttcgagt cgttgcgcga tgcggggacg agcgcggtct tgctcgaagt gccctccgtg
     4141 cgagtcgggc tcgcggagct cgagcgctcg cgacccgcgc tcgacgcctt ggggctcgtg
     4201 gccggcgtgc tgctcgctgc gagcgacaaa gcagtgcgag gcttcccaga cccgaccagc
     4261 gagccggagc aatgggcgga gcacgcgatc gacctttcac tcgccggcgc gcgagtgctc
     4321 ggcggtggtg ccggaacgac ggaatcgcat acgagggcgc tggcgttagc gctcggagcg
     4381 ctccatcctt ccgtcccacc ccctcgcgcc gcgtccgaaa tagatcggcg tccgcgggat
     4441 tggtgaccgc gcgcctggat gcaacgacaa tctctcgacg acaacgcccc gctgggtgac
     4501 acggacgcgc ttacgtccgt ctttcacgcg gcagagaagc cagtttccgc gttccggcta
     4561 ggcgccgaag gtgaaaagtt cggcgttcac gagctgaccg gggctccgct ctcttatgag
     4621 ggcgaacacg gcgtggcgcg cgtgctcgcg gcactcaccc gctttgggtg gcagcccgaa
     4681 agcgagaccg aaggcgggcc catcatcgcg ctgactcgcg gcgcagcttc agtgacgctc
     4741 gaaccgggct cgcagctcga gctgagcggt tctgcgctac ccgacgtgca cgccgtgcag
     4801 gcggagttcg cagagcacat gcaagagctc gccgacatct cgcgcgagat gcagctcgca
     4861 tggctcggcg ttggcttcca tcccatcgcg cagcaggcgg agctcacctg ggtgccgaag
     4921 cagcgctacg gcatcatgcg cgagtacttg ccgccgctcg gctcggccgc gcacgacatg
     4981 atgcgtcgca ccgccaccgt gcaggtgaac atggactacg cgagcgaaga ggacgcgatg
     5041 cgcaagctgc ggatcggcct cttgctggct ccgctgctca acgcgatgac cgcgaattcc
     5101 ccgttccggg agcgggcgcg caccgacaag aagagcctgc gcggcaacgt ctggctccac
     5161 atggatcctt ctcgcagcgg tctcgtgcgg cgggtgctcg cgaagaagcg ggccacgtat
     5221 cgtgacta
//
LOCUS       DASTKS010000752         5311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_566469, whole genome shotgun sequence.
ACCESSION   DASTKS010000752 DASTKS010000000
VERSION     DASTKS010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566469"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFQ35_15590"
     CDS             <1..414
                     /locus_tag="VFQ35_15590"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HET9932126.1"
                     /translation="YAAAAMSSLAASSFGADEPSGNDVVEFRTSVSNDKGVVRCGLFD
                     AGGWLKRPIRTAVAKIQGGTALCRFQRVPRGTFGASAFHDENSNGKLDTNFIGLPTEE
                     YCASRNARGTFGPPSFDDAKFQYRGGTKRIEARMK"
     gene            649..1383
                     /locus_tag="VFQ35_15595"
     CDS             649..1383
                     /locus_tag="VFQ35_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932127.1"
                     /translation="MQRSRSFVRFVWPAFAALVVLNCTVKVEDGDGHGGSSSASGGAA
                     NGGGGTANLSGGVANSSGGTAGASTGGAPVTSGGMASGGEAGAAQAQGGSGAMGGAGV
                     GGDGGSGGGPAADACEDCLLANCTAELEACLADSRCYSDDPDQPGQYQDMVQCIDQTR
                     VMHSVKRVDVVDCGLAIVNSSNLAWPPDEMASTTTDLINCMATGQTNKPNNNGWSNGD
                     PDVPTDPISQKWADGSCAKTSCTSQL"
     gene            1477..2133
                     /locus_tag="VFQ35_15600"
     CDS             1477..2133
                     /locus_tag="VFQ35_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932128.1"
                     /translation="MKRSQWIWALLLLTSASFAPAARAADPEALRQKLEKIKADREKA
                     RAAARATATPQPTAAPTPTPPPPPPPTATTTATSSAPKPTASAAASVAAPKPSAVPSA
                     IAPTASVAPSASAVPASVALSDVEQLRKSRPDRKHREAQNLRERWGELVNNEQAKAEL
                     RLHAQRGAYLQRIRQLAESKNDSKLVESIDKLITVEERRSADAMNALRTAALPSGGKP
                     "
     gene            2130..2429
                     /locus_tag="VFQ35_15605"
     CDS             2130..2429
                     /locus_tag="VFQ35_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932129.1"
                     /translation="MSRATLVFACALLCATGCKALLKKREPITTESSATAPQASAPVA
                     VAPAPPPPAPPPVAPVVDDAVIPTTQDFEDEALEKVTPANFRAELARLKKEIEKK"
     gene            2600..3694
                     /locus_tag="VFQ35_15610"
     CDS             2600..3694
                     /locus_tag="VFQ35_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932130.1"
                     /translation="MSENRRGTDLRVRVAFRVRFRSIDQLVMALTGDLSRGGMRLSSP
                     QKVEIGSRVSVNVSLPDAGPEMTVLCEVVTLTPHKSGEGFSIGVKFVDPNGDFQQRIE
                     WFILNSEPAERQFGQHPHRRQLKLVVADDDPLQAEATAKPFLGRGDNVLLAADGLEAL
                     GLCLKEKPDVLLTDVHMPKMDGWQLLRMVRARPQLAGIPVLFLTTLASESDHLLGYRL
                     GVDDYLAKPCAPSALLERIDRAALRATQAARSSQGHVEPGALRGDLQLVGLASVLSFL
                     ELERKSGVVRVGPDANGVIYLRDGVPVHIAVDGVSDDAAPEERFAPLISARSGRFEFR
                     PGAVDRADTVKRSLGMLLLECARRADEAAR"
     gene            3750..4976
                     /locus_tag="VFQ35_15615"
     CDS             3750..4976
                     /locus_tag="VFQ35_15615"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET9932131.1"
                     /translation="MSRSQLVRMSSAALAFAVASSAASVAGAHGLAMDQVLLQPDRAA
                     HVLRGQVTFDPHHSRGEARAIAPDTEQRVLSTLRNSLVVEIDGKRCPLELEVRELWEP
                     AGATVGDIVRLSCPLPVNARELQVFAGSALTVLIVSVQAPSGSDAARTVSVLVQGGAA
                     TPVYRFDRAPEAWREGGANQFLADGGLASTAPDVPAATPTAVASPMAVAPAASNGRFE
                     ESSGALFWRYVRLGFTHILPSGWDHVLFVTGLVLGASGRLRRLLIELSAFTLAHTMTL
                     GLGALGVFAASPRLVEPLIALSIAFIGFENLRAKPSPRGRLALVFGFGFLHGMGFASA
                     LAQIGLARDSFVLSLLSFNGGVELGQLAVVVVLMTLLAPVRDEGRLRRFVVRPASIAI
                     AGAGIAWALARVFGYA"
BASE COUNT          907 a         1694 c         1769 g          941 t
ORIGIN      
        1 tatgcggcag ctgcgatgtc gagtctcgcc gcatcgagct ttggtgcgga cgaaccttca
       61 gggaacgatg tagttgagtt tcgcacctcg gtgagcaacg acaagggtgt cgtgcgctgc
      121 ggcctcttcg acgcgggggg atggttgaag cgaccaatcc gcactgcagt cgcgaaaatc
      181 cagggcggaa cggcgctctg tcgctttcaa cgcgtccctc gcgggacctt cggagcttcg
      241 gcgtttcacg acgagaactc gaacggaaag ctcgatacga atttcatcgg tctgccgacg
      301 gaggaatact gtgcatcccg gaacgcccgc ggaacatttg gtcccccgag cttcgacgat
      361 gcgaagtttc agtatcgggg tgggacgaag cggatcgaag cgcggatgaa gtagtgaaga
      421 gcttttgctc gtaacgaaaa ttggctccac atcgcttcac cgagaacgag gtgactgcgc
      481 gactcgctga atgagcagcg ctagacgcgg cctcgaggcc gaccgtcctt tgtgcgcagc
      541 cggaaggtgg ggatcccaag ccgaacagtt tcgtatatga ctacggcctg ggattgagcc
      601 ctgccaagcg gcgtggtggt acgcaggggc tcgaagtagg agcaatcgat gcagcggtcg
      661 cgttcgttcg tgcggttcgt ttggccggct tttgcagctc tcgtcgtgtt gaactgcacc
      721 gtgaaggtcg aggatggcga cggacacggc ggttcatcga gcgcttctgg aggtgcggcg
      781 aacggcgggg gaggtaccgc gaatctcagc ggcggagtcg cgaactcgag cggcggaacg
      841 gcgggcgcct cgaccggcgg cgcacccgtc acctctggcg gcatggcgtc ggggggcgag
      901 gcgggagcag cccaagcgca gggcggcagc ggtgccatgg gaggagccgg cgtcggtggc
      961 gatggcggta gcggcggagg ccccgcggct gacgcgtgcg aggactgctt gctcgctaat
     1021 tgcaccgccg agctcgaagc gtgtctcgct gattcgcgct gttacagcga cgatccagac
     1081 caacccggtc agtaccaaga catggtgcag tgcatcgacc agacccgtgt gatgcactcg
     1141 gtcaaacgcg tcgacgtcgt cgactgcggc ctcgccatcg tgaacagctc gaacctcgcc
     1201 tggccgcccg acgagatggc gtcgactacg accgacctca tcaactgcat ggcgacgggc
     1261 cagacgaaca aaccgaacaa caatggctgg tcgaacggcg atcccgacgt tccgacggat
     1321 ccgatcagtc agaagtgggc cgacggcagc tgcgccaaga cgtcctgcac ctcacagctc
     1381 tgagcctcga aggttcttct tcgttcacct cctctcgcgg ctgaccgctg ctccgagatc
     1441 caagtggatc gaccgtccct ctcacgaggc aagctaatga aacgaagtca gtggatttgg
     1501 gccctgcttt tgctcaccag cgcaagcttt gctccagcgg cccgcgccgc ggatcccgaa
     1561 gcgctccgcc aaaaactcga gaagatcaag gctgaccgcg agaaggcgcg cgccgcggca
     1621 cgtgcgaccg caactccgca gccgacggcg gcaccgactc ccacgccgcc gccaccgccg
     1681 ccgcccacgg caacgacgac cgcaacgagc agcgcgccga agccgaccgc gtcggcagcc
     1741 gcatccgtag cggcgcccaa gcccagcgcg gtgccttccg cgatcgcgcc gaccgcatcg
     1801 gttgcgccca gtgcgtcggc cgtgccagcg agcgttgcgc tttccgacgt cgagcaactg
     1861 cgcaaatcgc ggcccgaccg caagcaccgt gaagcacaaa acctgcgcga gcggtggggg
     1921 gagctcgtga acaacgaaca ggcgaaggcc gagcttcgcc tgcacgcgca acgcggcgcg
     1981 tatcttcagc gcattcggca gctcgccgag agcaagaacg attcgaagct cgtcgagagc
     2041 atcgacaagc tcatcaccgt cgaagagcgc cgcagcgcgg acgccatgaa cgcgctccga
     2101 acggccgcgt tgcctagcgg aggcaagcca tgagtcgcgc aaccctcgtg ttcgcctgtg
     2161 cgctcctgtg cgcaactggc tgtaaagcgc tgctgaagaa gcgcgaaccc attacgaccg
     2221 aatcgagcgc gactgctcct caggcttcgg ctcccgtcgc cgtagctccg gcgcctcctc
     2281 ctcccgcgcc gccaccggtc gcaccggtgg tcgacgacgc cgtgattccc actacgcaag
     2341 acttcgaaga cgaagcgctc gaaaaggtga cgcccgcgaa cttcagggcg gagcttgcgc
     2401 ggttgaagaa ggaaatcgaa aagaagtaat tgtgattggg gcggcggcct tgtgcgacac
     2461 cgaggctcgc gcggcgccca catcgcttcg cgtgtgcacg gttccgcacc aaagctgatg
     2521 tgaggcagct ttgcgcgtga tacgttgaac cgaagaggcg acgtacgccg atgaatcact
     2581 gggtatcgaa aggggcgccg tgagcgagaa ccgccgcggg accgatctac gcgtccgtgt
     2641 ggctttccgc gtgcgcttcc gctccatcga ccaactcgtg atggcgctca cgggcgatct
     2701 gtctcgtggc ggaatgcggc tcagctcgcc gcaaaaagtc gaaatcggct cgcgcgtctc
     2761 ggtcaacgtc agcctgccag acgctggacc cgagatgacg gtgctgtgtg aggtggtgac
     2821 cctcactccg cacaagtccg gtgaggggtt cagcatcggg gtcaagttcg tcgatccgaa
     2881 cggtgatttt cagcaacgca tcgagtggtt catcctcaat tcggagcctg ccgaacgcca
     2941 attcggtcag cacccgcatc gccgtcagct caagctcgtc gtcgccgacg acgacccgct
     3001 gcaggccgaa gccaccgcaa agccgttcct cgggcgtggc gacaacgtct tgctcgctgc
     3061 cgatggcctg gaggcgctcg gcttgtgcct gaaagaaaag cccgatgtgt tgctcacgga
     3121 cgtccacatg ccgaagatgg acggctggca gctcttgcga atggtccgag cgcgaccgca
     3181 gctcgccggg atcccggtgc tgttcttgac gacgctcgcg agcgaaagcg atcacctgct
     3241 cggctatcgg ctcggcgtcg acgactacct cgccaagccg tgcgcgccga gcgcgttgct
     3301 cgagcgcatc gaccgcgctg cgcttcgcgc gacgcaggct gcgcgctcga gtcagggtca
     3361 cgtagaaccg ggagcgctgc gcggtgattt gcagctcgtc gggcttgcct cggtgctctc
     3421 gttcctcgag ctcgaacgaa agagcggcgt cgtgcgagtg ggacccgacg cgaacggcgt
     3481 gatttatctg cgtgacggcg tcccggtgca catcgcggtg gacggcgtct ccgacgacgc
     3541 ggctcccgag gagcgattcg cgccgctcat ctccgcgcgc tcgggccggt tcgagttccg
     3601 tcccggagcg gtggatcgcg cggatacggt gaagcgttca ctcggtatgc tcttgctcga
     3661 gtgcgctcgc cgggccgacg aagccgcccg ttgaacggag cctccggctt tcgcgccacg
     3721 aggattcgtg aataatgcgc ggcgctcgaa tgtctcgttc gcaactcgtc cggatgtcgt
     3781 ctgcggcact cgcctttgcg gttgcgagca gcgcggcgtc cgttgcgggc gcgcatggcc
     3841 tcgcgatgga ccaggtgctc ttgcagcccg atcgagcggc gcacgtgctt cgcggccaag
     3901 tcaccttcga tccccatcac agtcgaggtg aggcgcgagc gatcgcgccg gacaccgaac
     3961 agcgcgtgct ctcgactctg cgaaatagcc tcgtggtgga gatcgacgga aagcgctgtc
     4021 cgctcgagct cgaggttcgg gagctctggg agcctgccgg agcaaccgtc ggcgacatcg
     4081 tgagactgag ctgcccgttg cctgtgaacg cacgtgagct acaggtcttc gctggctcgg
     4141 cgctgaccgt cttgatcgtc tccgtgcagg cgccctctgg ttccgacgcg gcgcgcaccg
     4201 tcagcgtgct cgtccagggc ggtgccgcga cgccggtgta tcgcttcgac cgcgctcccg
     4261 aggcttggcg agaaggcggt gcgaaccagt tcctcgcgga cggcgggctt gcctcgacgg
     4321 cgccggacgt acccgcggcg acgcctaccg cggttgcatc gcccatggcg gtggcacctg
     4381 cggcgagcaa cggacgtttc gaggagagca gcggggcact cttttggcgc tacgtgcggc
     4441 tcggcttcac gcatatcctt ccgtccgggt gggatcacgt attgttcgtt accgggctcg
     4501 tgctcggagc gagcggacgg ctgcgacgtc tgctcatcga gctcagcgct ttcacgctgg
     4561 cgcacacgat gacgctcggg ctcggcgcgc tcggtgtttt cgcggcgtcg ccgcggctcg
     4621 tcgagccgct catcgccctc tcgatcgcgt tcattgggtt cgaaaacctg cgcgccaagc
     4681 cgagtcctcg ggggcgcctc gccctcgtgt tcgggttcgg tttcctccac ggcatggggt
     4741 tcgcttctgc gctcgcgcag atagggctcg cccgagactc tttcgtcttg agcttgctgt
     4801 cgttcaacgg cggcgtagag ctcgggcagc tcgcggtcgt ggtggtgctg atgacattgc
     4861 tcgcgccggt tcgcgatgaa gggcggctgc ggcgcttcgt ggtgcgtcca gcatcgatcg
     4921 cgatcgcggg cgcagggatc gcctgggctt tggcgcgagt attcggttac gcctgagctc
     4981 gcgctgcgct tcccgactgg ctcgagccct acgcggacgg ctggtcgcgg cggaaaattg
     5041 cagtcgaacg gtaggtgtcg ttcgtgcgat cggcaagcag caagatcgta gagtcagcgc
     5101 ccctatcctt tgcgagtgcg tcgatgagct tcgcgaagtt cgtgcggcat ctataccaat
     5161 gaaagcaatg agacacctcg ggctccacac ggcgattcgt ccgaactcgg tcggccccta
     5221 cgtggcgctg ctcctgctct cgacgagcta tgcatgcagt tccggcgatt ccccgggtgg
     5281 cggcagtggc gggcgccaga tgaatggctc c
//
LOCUS       DASTKS010000753         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1907086, whole genome shotgun sequence.
ACCESSION   DASTKS010000753 DASTKS010000000
VERSION     DASTKS010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907086"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFQ35_15620"
     CDS             complement(<1..617)
                     /locus_tag="VFQ35_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932132.1"
                     /translation="MVTPSTILIVEDEPSFGQALGRWLEMLGHDSLLVGTAEEAASVY
                     AARRFKSILLDLHLPGRSGHVFLRQLCGVNSQTPVIVISGGAQMDDVILALRNGAADF
                     LRKPFRIDELAQALARATDDRSALNRLLSLAKDVALPTPELTAAPGTSGAAAPAPEPA
                     RAVAVPIAERTLPSRPRTVQALLEQIRRGVASLPVLDSQVGRIAQL"
     gene            complement(626..4105)
                     /locus_tag="VFQ35_15625"
     CDS             complement(626..4105)
                     /locus_tag="VFQ35_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9932133.1"
                     /translation="MVDSPCVLLVSSDVDRHRSLADTVAAAGFGTVCVALRELCEDEL
                     RFATPSVCLLACSSEAELEVVARYRGATGVKSTPWLVVTPSAEPRLREGALRRGADDV
                     LDDSASPFELGAKLRLLTELSTLRTQASPHESDSAEILNRIRAVASRLPLLMYEVDVV
                     GPSQMRGIWMLGGLARSADIDARAFAEGSNWEERIHPDDRAHVKGSLGASLEGHTELH
                     YEFRLRRRDGGYDWVSNVSLFEPERRRRSGILLDITKQKQAEERLAHHQKMEALSVLA
                     GGIAHDFNNILAVILSFVGFASEGLAPTDRRCADLNEVVKAAHRAARLTRQLLTVTSQ
                     QPAEIEPIDLNHRLGELATLMGAAAGPSVRIIVVNSLGPAIVELDPAQLDEVVLNLVM
                     NARDAMPEGGRLTIEIAGPYTDALGRDMVRLEITDTGIGMDARTLQRVFEPFFTTKVK
                     GGGAGLGLATCFGIVDAAGGRISAHSEPGKGSTFTVELPSSRRAPASPLASNGHEPRG
                     HGETVLLVEDEEVLLRALVRVFEGAGYRVRSAVDGSTAKKIIDELGASLDALVTDVII
                     PQVGGNEVAHHAKSVAPNATIFLTSGHVDGARVDGDFPILWKPIPPREIARAVTAALE
                     LRGGARRVADSSAKTVLVIEDDPAAGQALTRLLAGAGFEPRLLTTVAESRRYLETQRP
                     PTLVLCDLGLPDGSGSAVLESFAQKWPQLRSRLFALSGAFGNAEDRRALAERGVRVLS
                     KLTPPAQLLELLGASGAASEAPTPSSRAPSALDIAASPAGARHTRERVLVLERDVAMA
                     QASARMLQSAGFDVTIAASRSDATKRYATARFDALVLDASLAAPELDSLPALPAVILG
                     AAPGTAGVAVARRSATEQLRKPFSPAELQAAARNVIDAERSARVRRQLAATHLGGDQY
                     QADLTLTGRAFESALARVYVAFQPIVRAENDSIYGFEALLRCHDEAFSTPARLLAAAQ
                     VLGRQRELGLSVRRAIADALSAHPERDETIFVNIDAHELDDGTLYDPQEPLLSRAARI
                     VLEVSERASLGVADTRLERKLSRLRERGYRFALDDLGEGHVGLSSLVALKPDIVKIDP
                     ALVRRIETSSLKRDLVGSIVQAARRSGILVAAEGIESREERDTMIALGCDLLQGYFFA
                     RPAPSFPALERPI"
     gene            4345..5844
                     /locus_tag="VFQ35_15630"
     CDS             4345..5844
                     /locus_tag="VFQ35_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932134.1"
                     /translation="MSSVSEGAPGLVSVLDGVPSAMVQWNRMGRVTAWNRAATRLFGF
                     SREEALGAHVDRFLAPAYRGVLTKVWQEFEGHSECSVHCQILTVGGESVPTEWHSIAL
                     TAPDGSVDRASTSVTVSHGGPRSDAEGGGALSLLASGVAHEFMNVFAVILSHAKFIEA
                     ASHGDPRQGDANEIVKSAATGACLTRHLLSISGLGSNHGRLDVNATVQAMAAAITSAM
                     GGAFELVVTTGDEAVVDMDWAEFDRMLLDLVLAARRRMPRVERLRISTERTVRSEGGS
                     LVRISVAPQGPEGEAPLPVRGAEFDCSEAKRIAESAEGTMGVRGAELFVELPCSGPAE
                     ETSSSGIGRGAQPPAAELKDTVFVLEDEDSVREALVRVLELAGYEVRASGSVASARAE
                     LESGLVPDLIVCDLSLHDGSSTKLLEWLRRERPAVYERAIVLTGGAITTADRSYLRRN
                     ALRVLNKPVGPGSLLSALADARARGGQPLRRGETHRPESARVSAKEGLP"
     gene            5841..>6141
                     /locus_tag="VFQ35_15635"
     CDS             5841..>6141
                     /locus_tag="VFQ35_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932135.1"
                     /translation="MTDASKVRAHFAALPSDAKPPAAELLAQISHDIKGPIATLAMEC
                     FSARMVLRKARQNASGEHVQTLAKQLEEISNNLERASTQLSDYLAALTTLRDADKD"
BASE COUNT         1050 a         1984 c         2088 g         1019 t
ORIGIN      
        1 agctgcgcga tgcgcccgac ttgggaatcg aggaccggta acgacgcgac gccgcgtcgg
       61 atctgctcga gcaatgcctg cacggtgcgc ggacggcttg gcagagtgcg ctcggcgatg
      121 ggtaccgcga ctgctcgcgc gggctccggc gcaggcgcgg ctgcaccaga cgtgcccgga
      181 gccgcggtga gttccggcgt cggcaaagcg acgtccttcg cgagtgaaag cagcctgttc
      241 agcgcggatc tgtcgtccgt ggcgcgcgcg agagcctgcg cgagttcgtc gatgcggaac
      301 ggcttgcgca gaaagtctgc cgcgccgttg cggagcgcca ggatcacgtc atccatctgg
      361 gcgccgccgc tgatcacgat gacgggtgtt tgcgagttga cgccgcagag ttgccgcaag
      421 aacacgtgac ccgagcggcc cggcaagtgc aggtcgagta ggatcgactt gaaccgtcgg
      481 gccgcgtata cgctcgccgc ctcctctgcg gtaccgacga gcagcgaatc gtgccccagc
      541 atctcgagcc agcgtcccaa ggcttggccg aacgatggtt cgtcttcgac gatcagaatc
      601 gtggagggcg tgaccatgct cgagctcaga tcgggcgttc cagcgccgga aacgagggcg
      661 cgggccgtgc gaagaagtag ccttgaagta gatcgcagcc gagagcgatc atcgtatcgc
      721 gctcttcacg cgactcgatc ccctccgccg cgacgaggat gccgctccgc cgcgccgctt
      781 gcacgatgct gccgaccaag tcgcgcttga ggctcgacgt ctcgatacga cgaaccagcg
      841 ccggatcgat cttgacgatg tcgggtttca acgcgaccag gctcgagagg cccacgtgcc
      901 cttcacccaa gtcgtcgagc gcgaaccgat agcctcgttc tcgtaggcgg ctcaacttgc
      961 gctcgaggcg cgtgtcggcg acgcccaagg aggcgcgctc gctcacttcc agcacgatcc
     1021 gcgcagcgcg cgacagcaaa ggctcttggg ggtcgtagag ggtcccgtcg tcgagctcgt
     1081 gcgcgtcgat gttcacgaag atcgtttcgt cgcgctcagg gtgagcgctc aaggcgtccg
     1141 cgatcgcgcg gcgcacgctg aggccgagct cgcgttgcct gccgagcact tgcgccgcgg
     1201 cgaggagccg cgcgggcgtg gagaacgctt cgtcatggca gcgcagaagc gcctcgaagc
     1261 cgtagatcga gtcgttctcc gcgcgcacga tcggctgaaa cgcgacgtag acccgcgcca
     1321 aagcgctctc gaaagcgcgc ccggtcaagg tgaggtccgc ttgatactgg tcacctccga
     1381 ggtgcgtcgc cgcgagttgc cggcgaacgc gcgcagagcg ctcggcgtcg atgacgttgc
     1441 gcgccgccgc ctgcagctcg gcaggtgaga acggctttcg cagctgctcc gtagcgctcc
     1501 tgcgagcgac cgcaacgcct gcggttccgg gagccgctcc caagatcacc gccggcaacg
     1561 caggcaagct gtcgagctcg ggcgcagcca gagaggcgtc gagcacgagc gcatcgaagc
     1621 gggccgttgc gtagcgtttg gtcgcgtcgg agcgtgacgc ggcgatggtg acgtcgaagc
     1681 cagccgattg cagcatgcgc gcgctggcct gggccatggc gacgtcgcgt tcgagcacca
     1741 gcacgcgttc gcgggtatgc cgtgctcccg cagggctcgc cgcgatatcc agggcactcg
     1801 gcgctcggga tgaaggcgtc ggcgcttccg acgccgcgcc cgatgcaccg agtagttcga
     1861 gcagctgggc gggcggcgtg agcttggaga ggactctcac cccacgctcg gcgagcgcgc
     1921 gtcgatcttc ggcgttcccg aacgcgcccg agagcgcgaa tagccgcgag cgaagctgcg
     1981 gccatttttg cgcgaacgac tcgagcaccg cactgccgga gccgtcgggc aaaccgaggt
     2041 cgcacagcac gagcgtcggt ggtctttgcg tctcgaggta gcgtcggctc tccgctacgg
     2101 tcgtcaagag ccgtggctcg aacccggctc cggccaacaa gcgagtcaga gcctggcccg
     2161 cggccggatc gtcctcgatg acgagcacgg tcttcgcgct cgagtctgcc actcgtctcg
     2221 cgccgccgcg cagctcgagc gccgccgtga cggcacgcgc gatctcacga ggagggatgg
     2281 gcttccacag gatcggaaag tcgccgtcga cgcgcgcgcc gtcgacgtgg cccgaagtca
     2341 ggaagatggt agcgttcggc gccacgctct tcgcgtggtg cgcgacctcg ttgccgccga
     2401 cctggggaat gatgacgtcg gttaccagcg cgtcgaggga ggcgccgagc tcgtcgataa
     2461 tcttcttcgc cgtggagccg tcgaccgcag agcgcacgcg atagccagcg ccttcgaaga
     2521 cacgcacgag cgcgcgcagc agcacctctt cgtcttccac gagcagcacc gtttcgccgt
     2581 gcccgcgcgg ctcgtggccg ttgctcgcga gcggggaggc gggcgcgcgt ctcgagctcg
     2641 gcagctcgac ggtgaaggtt gaacccttcc cgggctcgct gtgcgcgctg attcggcccc
     2701 ccgccgcgtc gacgatgccg aagcacgttg cgaggcccaa gccggcgccc ccgcctttga
     2761 ccttggtcgt gaagaacggc tcgaacacgc gctgcaatgt gcgagcatcc ataccgatgc
     2821 cagtgtccgt gatctcgagg cgtaccatgt ctcgccccag cgcatcggta taagggccag
     2881 caatctcgat ggtcagtcgt ccgccctccg gcatcgcgtc gcgcgcgttc atcaccaggt
     2941 tcagcacgac ctcgtcgagc tgcgccgggt cgagctcgac gatcgcgggg cccagactat
     3001 tcaccacgat gatgcgcaca ctcgggccgg cggcagcgcc catgagcgtc gcgagctcgc
     3061 ccaaccgatg gttcaagtcg attggctcga tctctgctgg ttgctggctc gtcaccgtga
     3121 gaagttgacg agtcagccgc gccgctcggt gggcggcctt cacgacctcg ttgagatcgg
     3181 cgcatcttcg gtccgtcggt gcgaggccct cgctcgcgaa tccgacgaac gacaggatga
     3241 cggcgaggat gttgttgaag tcgtgagcga tcccgcccgc gagcaccgac agggcttcca
     3301 tcttctggtg atgtgcgagc cgctcttcgg cctgcttttg cttggtgatg tcgagtagga
     3361 tgccggagcg acggcgccgc tcgggctcga agagcgacac gttcgagacc caatcgtatc
     3421 cgccgtcgcg gcgtcggagc cgaaactcgt aatggagctc ggtgtggccc tcgagtgacg
     3481 cgccgagcga ccccttgacg tgcgcgcggt cgtcggggtg aatacgctct tcccagttcg
     3541 aaccctccgc aaacgcgcgt gcatcgatgt ccgcggagcg cgcgagcccg ccgagcatcc
     3601 agatgccgcg catctgcgag gggccgacga cgtccacttc gtacatcaat agcggcaggc
     3661 ggctcgcgac cgcgcgaatg cggttcaaga tctcggccga atcgctctcg tgcggagacg
     3721 cctgcgttcg aagcgttgag agctcggtca agagccgcag cttcgcgccg agctcgaaag
     3781 gcgacgcgga atcgtcgagg acgtcgtctg cgccacgacg cagcgcccct tcgcgcagcc
     3841 gcggctcggc gctcggtgtg acgacgagcc aaggcgtcga cttcacgccc gttgcgccac
     3901 gatatcgagc tacgacctcg agctcggctt cgctcgagca cgcgagcagg cacacagaag
     3961 gcgtggcaaa gcgtagctcg tcctcgcaca actcgcgcag cgccacacaa acggtaccaa
     4021 agcctgcggc cgcaacggtg tctgcaagcg accggtgccg gtcgacatcg ctcgagacca
     4081 gcaagacaca cggggaatca accatcggcg cttcgggaag aaggagtgcg cctgctttca
     4141 ccgctcattc gagagcgata ctcgccagcg cgctgccttg ctcctcgaca ttacgccaca
     4201 ctcgcggcgc atctttacct gcctacatag gcaccccgct gagtacgcta atatcgttgg
     4261 cgggcgttac gaagtcgtcc tgtgcgacga gccgcgcagc gattgcgtgc acagtcgagc
     4321 gtacgagggt aaagtaaagt ttcaatgagc tccgtgtcgg aaggcgcgcc cgggctcgtt
     4381 tccgtattgg acggcgttcc cagcgccatg gtccagtgga accgaatggg ccgagtaact
     4441 gcgtggaatc gagcggctac gcgactcttt ggattcagtc gcgaagaggc gctcggcgcc
     4501 cacgtcgatc ggttcctagc gcccgcgtat cggggcgttc tgacgaaggt ctggcaggag
     4561 ttcgaggggc actccgagtg ctccgttcat tgccaaatcc tcacggtcgg cggggaaagc
     4621 gtgcctacgg aatggcacag catcgccctg acggcgcccg acggttccgt cgaccgtgct
     4681 tcgaccagcg tcaccgtgtc acacggaggg cctcggtccg atgccgaggg tggcggcgcg
     4741 ctgagccttt tggcatccgg agtagctcac gagttcatga acgtgttcgc ggtgatcctc
     4801 tcccacgcga agttcatcga agcggcgtct cacggcgacc cacgccaggg tgatgcgaac
     4861 gaaatcgtga aatcggcagc cacgggagcc tgcctcacga ggcacttgct cagcatctcg
     4921 ggccttggct cgaatcatgg gcgactcgac gtgaacgcaa cggtgcaggc gatggcggcc
     4981 gcaatcacgt cggcgatggg cggcgccttc gagctcgtgg tgaccacggg ggatgaggcg
     5041 gtcgtcgaca tggattgggc ggagttcgat cgtatgttgc tcgacctcgt gctcgccgcc
     5101 cggcgtcgga tgccgagagt cgaacggctc cgtatcagca cggagcgaac ggtgcggtcg
     5161 gaaggaggct cgctcgttcg catcagcgtc gcgccgcaag gacccgaagg agaggctccg
     5221 ttgccggtcc gaggcgcgga gttcgactgc tcagaagcaa agcgaatcgc agagtcggcg
     5281 gagggaacca tgggcgttcg cggggcagag ctgttcgtcg aattgccgtg tagtgggccc
     5341 gccgaggaga cctcttcgag cggcatcggt aggggcgcgc agccccccgc cgccgaactc
     5401 aaggatacgg tgttcgtgct cgaagacgaa gacagcgtgc gagaggcgct ggtacgggtg
     5461 ctggagcttg ccggttacga ggtgcgagcc agcggtagtg tggcatcagc gcgggctgag
     5521 ctcgagtccg gcttggttcc tgacctgatt gtgtgcgatc tgtcgttgca cgacggctcc
     5581 agcaccaagc ttctcgaatg gctccggcgt gaacggccgg cggtctacga gcgggcgatc
     5641 gtgctcacgg ggggcgccat caccaccgcg gaccgcagct acctccgcag gaacgcgttg
     5701 cgggtgctca acaagccagt cggacctgga tcgctgttga gcgcactagc cgacgcgcgc
     5761 gctcgaggag gccagcctct acggcgcggc gaaacgcatc gcccggagag tgctcgtgtg
     5821 agcgcgaagg aagggctgcc gtgaccgacg cttccaaggt tcgcgcgcat ttcgcggctc
     5881 tcccctcgga tgcgaagcct cccgcggcgg agcttctcgc gcaaatctcg cacgacatca
     5941 agggccctat cgcgaccctc gccatggagt gcttttcggc gaggatggta cttcgaaaag
     6001 cgcgccagaa cgcgtctggc gagcacgtgc agactctcgc aaagcagctc gaggagattt
     6061 cgaacaatct cgagcgtgcg tcgacccagc tgagcgacta cttggcggcg ctgacgacct
     6121 tgcgcgacgc ggataaagac t
//
LOCUS       DASTKS010000754         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_121053, whole genome shotgun sequence.
ACCESSION   DASTKS010000754 DASTKS010000000
VERSION     DASTKS010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121053"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..574)
                     /locus_tag="VFQ35_15640"
     CDS             complement(143..574)
                     /locus_tag="VFQ35_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932136.1"
                     /translation="MSNLRISRCPRARKFSRGAAFVEALVASVALAGLVTAGFLLFRK
                     WERAASYLDERARTFAAAFGGCGRRRDLSSEASLAWHAFDPRAFVGAEPSKRAFSVSS
                     NADYPGERRESVACNERAVLFPAELTRLRHDIERQLLGRSK"
     gene            complement(595..>2340)
                     /locus_tag="VFQ35_15645"
     CDS             complement(595..>2340)
                     /locus_tag="VFQ35_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932137.1"
                     /translation="LSLEDTRGAILVVGLVLGFALALAVHTLVGVDSAVLDRETVQSA
                     ADAAAFESAVWHARGMNSLAGLNVLLALVLATLVFWRLATFAVGLAVLLESVALVQQP
                     GSARTQIRELTPFAELLEYDGHVAGSVLLAASGLRDAEAAVAAYTPVLAAHAAALHTR
                     ESYDVVARTFSASLLPNVQTSAPAALAACLRVAAASSNESGTAADGESRSGGTDLSGL
                     RQRAEEDVTSMLAFAERGPALPSASARLGMPFSLPVEVGANAMLCERATEVARDGEPV
                     HGAPPSIANALNANEHISGRKQRALVALGVTSKEERLRRLGEALSGVLDAKAPAAFCA
                     STGAGVDALHHSMAKLASDLREYAADDRTRTMLARLGSGRATLLGGPQSLEEARAIWS
                     AWRRALEAGGDPSRCIRPVEVWSPARNGTGFFRSASAVAMPTAPFGLRSPGEVGASVD
                     FRATAHAETFFDCEDSWDRCRGNALWEPSWSARLRRVRSFGALLDAGESTPNFTVADA
                     NVMARWVERLARRIGQPRVLFDVLAERAELEVEPDPVARTWSVGLLQAQASAQAQRPL
                     AGFVARHAKESSIVH"
BASE COUNT          426 a          823 c          736 g          355 t
ORIGIN      
        1 tcgagcgcac tgttctccgc gtacgcgcta gccaggcggc tgccgagcag cagcgtgccc
       61 atcccgatca cggcagcgcg cacgaaacgt gcgaaccagc gtcggcaggc gcgctcgcag
      121 ccgggactcg aggtcgcttc agtcatttgg acctccccag taactggcgc tcgatgtcgt
      181 gccgtagccg tgtcagttct gcagggaata ggactgcccg ctcgttgcac gcgacgctct
      241 cacgacgctc cccagggtag tccgcgttgc tcgaaacgct gaatgcacgt ttcgatggct
      301 cggctccaac gaaggcgcgg ggatcgaagg cgtgccacgc gagcgaagcc tcggaggaga
      361 ggtcccgtct gcgcccgcat cccccaaacg cggccgcaaa agttcgagcg cgctcatcga
      421 ggtacgacgc cgcgcgttcc cacttgcgaa acagcaagaa cccagccgtg acgaggccgg
      481 cgagcgcaac gctcgccacc aacgcttcga cgaacgcggc cccgcgcgag aacttccgcg
      541 ctcggggaca acgactgatt cgtagattgc tcattgcgat tcgaccacgt aatttcagtg
      601 aacgatgctg ctttccttgg cgtgacgcgc cacgaagcca gccagcggac gttgagcctg
      661 cgcgcttgcc tgagcctgca gcaggccgac gctccaagtg cgagcaaccg ggtcgggttc
      721 cacctcgagc tcagcacgct cggcgagcac gtcgaacaga acccgaggtt gcccgatgcg
      781 tcgcgcgagg cgctcgaccc agcgtgccat gacgttcgcg tcggccaccg tgaagttggg
      841 cgtgctctca cccgcatcga gcaatgctcc aaacgaacgc actcgtcgca agcgcgccga
      901 ccagctcggc tcccagagcg cgtttccgcg gcacctatcc cagctatcct cgcagtcgaa
      961 gaatgtttcg gcatgcgcgg tagcgcggaa gtcgacgctc gcgccaactt caccggggga
     1021 ccgcaaccca aagggtgcag tcggcattgc gacggcgctc gcgcttcgaa aaaagccagt
     1081 cccgttacgc gcgggcgacc acacctctac cggtcgaatg cagcgactcg gatctccgcc
     1141 cgcttccagg gcgcgtcgcc aagcgctcca aatcgcccgc gcctcctcga gcgactgcgg
     1201 gcctccaagc aacgtggcac gaccggagcc gaggcgtgca agcatggtcc gggtgcggtc
     1261 gtccgcggcg tactcacgaa gatcgctcgc gagctttgcc atgctgtggt gcaaagcgtc
     1321 cacacctgca cccgtcgatg cacagaaggc tgcgggcgcc ttcgcatcga gtacgcccga
     1381 cagcgcctcc cccaaacgcc gaagccgctc ttccttcgag gtgactccaa gtgccacgag
     1441 agcacgctgt ttcctgccag aaatgtgttc gttcgcgttc agggcgttcg ctatcgacgg
     1501 cggggctcca tgcactggct cgccatcacg ggcgacctcg gtagcgcgtt cgcagagcat
     1561 cgcgttggct cccacctcga ctggcagact gaaaggcatt ccaagccgcg cgctcgcgct
     1621 cggcagtgcc ggccccctct ccgcaaaggc gagcatcgac gtaacgtctt cttctgcgcg
     1681 ctggcgaagg cctgaaaggt ccgtacctcc gctgcgcgac tccccgtccg cggcggtgcc
     1741 agactcgttg ctcgaagctg cggcgacgcg caggcacgcc gctagagccg ccggcgcgct
     1801 ggtttgcacg ttcggcagca acgacgcgct gaacgttcgc gcaacgacgt cgtacgattc
     1861 tcgcgtgtgc aacgcggccg cgtgcgcagc aagcaccggc gtataggccg ccaccgccgc
     1921 ctcggcgtcg cgcagccccg acgcggcgag aaggacgcta ccggcgacgt ggccgtcgta
     1981 ctccagcagt tccgcgaagg gagtaagctc gcggatttgg gtccgcgccg atcccggctg
     2041 ctgcaccaaa gccaccgact cgaggagaac cgcgagccca actgcgaacg tggcgagccg
     2101 ccaaaatacc aatgtggcga gcacgagcgc gagcagtacg ttcaggcccg cgagcgagtt
     2161 catgccgcgc gcgtgccaga ctgcgctttc gaacgcggcc gcgtccgcgg cgctctgaac
     2221 cgtctcgcga tcgagaacgg cgctatccac tccgaccagg gtgtgaaccg cgagcgcgag
     2281 cgcgaagccg aggacgaggc ccaccaccag gatcgcgccg cgagtatcct cgagggagag
//
LOCUS       DASTKS010000755         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1907567, whole genome shotgun sequence.
ACCESSION   DASTKS010000755 DASTKS010000000
VERSION     DASTKS010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907567"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VFQ35_15650"
     CDS             <1..1322
                     /locus_tag="VFQ35_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932138.1"
                     /translation="VTNVVTGAYMPAISPDGKTLVYVGYTSKGFDLYRMPIDATNWLD
                     PLPPPERPDGVVVTPTKHYPVARYNPLPTVRPRAYDLTYGTGNFGRNALTISTKGSDA
                     VGHHVYGASVTFEPNGPEWQGGLDYWYTRLPFDFHASVFRSTQPQRNYRVGETPETVV
                     EHAYGVSTGLELFVPSEFNWQAAALSYNVTDTFHDAPFGTRVDPWAPVPTEPSSGFVT
                     WVHAGYAFGNAEGSTYSIKFERGLALNLGVDWSGPALGSESTFSVFSGNVIGYVLMPW
                     RHHHVLALQAMAGSSAGTWARRAGFATGGYVDQPVVDVYTKGIRQPQFVLRGFEPLQF
                     FGNNFTLFNAEYRFPIAYVDRGVSTLPVFLQQVSGTVFADWGGAYDSIPHDSPLDVMH
                     LGAGGELWVNFSLGYRLDWMLRIGLAHGFGAAAKNPIQSYFVISTAY"
     gene            1376..1846
                     /locus_tag="VFQ35_15655"
     CDS             1376..1846
                     /locus_tag="VFQ35_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932139.1"
                     /translation="MRALFCSLALAATACLAPPAASERATDAARSLNVAARFGRMDVA
                     LGLTSDAVRKTFLEHRASWGKDLRVLDVELVGFEMAASDRAKVEVEYAWSRLNESLLR
                     STRVTQEWRDAGGGFRLVREKRSSGDLGLFGEALPAQVEPSPRRDAQFATKVIQ"
     gene            complement(1875..2012)
                     /locus_tag="VFQ35_15660"
     CDS             complement(1875..2012)
                     /locus_tag="VFQ35_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932140.1"
                     /translation="MGKFDRRNSQKMTRRKAQRKKKARLARKADAARAERSKKKPSKK
                     R"
     gene            complement(2191..2442)
                     /locus_tag="VFQ35_15665"
     CDS             complement(2191..2442)
                     /locus_tag="VFQ35_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932141.1"
                     /translation="MQVVLRKLGRGSRAVAGRLVRAPRKGSVVVIEFSDGMHEYVTTP
                     VKRVLRLATREVFYIETVNSRYRLEVRDRERALEDASSG"
     gene            complement(2672..>2870)
                     /locus_tag="VFQ35_15670"
     CDS             complement(2672..>2870)
                     /locus_tag="VFQ35_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932142.1"
                     /translation="VLGHESGLSHVARGWLVADAGYLYTASTDLVYATSGGSPARAAP
                     ISLGELGLHGATLRVTAAVSF"
BASE COUNT          467 a          978 c          896 g          529 t
ORIGIN      
        1 aagtcaccaa cgtcgtgacc ggcgcgtaca tgcccgccat ctcgcccgac ggcaaaacgc
       61 tcgtctacgt cggctacacg agcaaaggct tcgacctcta tcggatgccg atcgacgcta
      121 ccaactggct cgatccgctt ccgccgccgg agcgcccgga cggcgtcgtc gtgactccga
      181 cgaagcacta cccagtcgcg cgctacaacc cgctgccgac cgtgcgcccc cgcgcttacg
      241 atctcaccta cggcaccggg aacttcgggc gcaacgcgct aacgatctct accaagggct
      301 ctgacgcagt cgggcaccat gtttacggag catcggttac gttcgagccg aacggccccg
      361 aatggcaggg aggcttggac tactggtaca cccgactgcc gttcgacttt cacgcgagcg
      421 tgttccgcag cacccaaccg caaaggaact atcgcgtcgg ggagacgccc gagacggtgg
      481 tcgagcatgc ttacggggtt tcgaccggcc tcgagctgtt cgtgccttcg gagttcaatt
      541 ggcaggcggc tgccctctcc tacaacgtca ctgacacctt ccacgatgcg ccgttcggca
      601 cgcgcgtcga tccatgggcg ccggtaccga cggagccatc ttctggtttc gtcacttggg
      661 tccacgcggg ttacgcgttc ggcaacgccg agggcagcac ctactccatc aaattcgagc
      721 gcggcctcgc gctgaacctg ggcgtggact ggtctgggcc agcgctcggc agcgaatcga
      781 cgttctccgt gttctccggg aacgtcatcg gctacgtgct catgccctgg cgccaccatc
      841 acgtgctcgc gctgcaagcg atggcgggga gctccgccgg aacttgggcg cgccgcgccg
      901 gcttcgcgac gggcggatac gtcgaccaac cggtagtcga cgtgtacacg aagggtattc
      961 gtcagccgca gttcgtactc cgcggcttcg agccgctgca gttcttcggc aacaacttca
     1021 ccctgttcaa cgccgagtac cggtttccaa tcgcgtacgt cgatcgcggc gtgagcacgc
     1081 ttccggtctt cttgcagcag gtgagcggca ccgtcttcgc cgattggggc ggggcttacg
     1141 attccattcc ccacgattcg ccgctcgacg tcatgcatct gggagccggc ggcgagctct
     1201 gggtgaactt ttcgctcggc tatcggctcg actggatgtt gcgcattggc ctcgcccacg
     1261 gcttcggcgc ggccgcgaag aaccccatcc agagctactt cgtcatcagc accgcgtatt
     1321 gaagcgcagc gggtttcttt cccgccgctc tcgagccctg ctatgcgctc gagagatgcg
     1381 cgctctcttt tgctctcttg ctctggcggc gacggcttgc ctcgcgcctc cggctgcctc
     1441 cgagcgcgcg acggacgccg cacgctcgct caacgtggcg gcccgcttcg gtcgcatgga
     1501 cgtcgcgctc gggctgacct ccgacgcggt tcgcaagacg ttcctcgagc atcgcgcgag
     1561 ctggggcaaa gaccttcgcg tgctcgacgt agagctcgtc ggcttcgaga tggcggccag
     1621 cgaccgcgcg aaggtcgagg tcgagtacgc ctggtcgcgg ctgaacgagt ccctgctgcg
     1681 ctcgactcgc gtcacgcaag aatggcgcga cgcgggaggc ggtttccgcc tcgttcgcga
     1741 aaagcgcagc tccggtgacc tcggcttgtt cggcgaagcg ctccccgcgc aagtcgagcc
     1801 gagcccacgg cgggacgcgc agttcgccac gaaggtgatt cagtagcgag gcgcgccgag
     1861 ccgcgagcag agcttcagcg cttcttcgag ggcttcttct tcgagcgctc ggcgcgggcg
     1921 gcgtcggcct tacgcgcgag gcgcgccttc ttctttcgct gagccttgcg gcgcgtcatc
     1981 ttctgcgagt tgcggcgatc gaactttccc atcggcggct attcctcagt aactcaggcg
     2041 cgctcggaag cgagccgtgc cccaccacgg ggcgtcgggg gcttaccttg cttttcgccg
     2101 cagcggaagt ctgaagtttc gtcgagccaa aggataggcg acgaacactt acgaaaaacg
     2161 aacgtcggtg cgcgccgctg cgcgcggtgg tcagccgctc gaggcgtctt cgagcgcgcg
     2221 ctcgcggtcg cgcacttcga gacggtaacg gctattgacg gtttcgatgt agaagacctc
     2281 gcgcgtggcg agtcgcagga cgcgcttgac gggagtggtc acgtattcgt gcatcccgtc
     2341 ggagaactcg atgacgacga ccgagccctt gcgcggtgct cgaacaagcc tgccagcgac
     2401 cgctcttgat cctcgtccaa gctttctaag cacgacctgc acgactctta tcctagcaac
     2461 ctcggcccct gcgtccacca gcattcagcg tcggatcgcg aggacgcgac gtctgtctcc
     2521 cacctcgacg catcggcgct tcccggacta atcgtcagca cgccgcgcaa attccaattc
     2581 tggcggacga tttgccgggt gtcacctaca cgacacggct cggcgcccac aagccccgcg
     2641 cgaaacgaca tgttcacagc ggttcgcatg ttcagaagct gacggcggcc gtgacccgca
     2701 gcgtcgcccc atggaggcct agctctccga gcgaaatcgg cgcggcgcgg gcgggagagc
     2761 cgccgctcgt cgcatagacg aggtcggtgc tcgccgtgta gaggtagccc gcatccgcca
     2821 cgagccagcc gcgcgcgacg tgactgaggc cgctctcgtg gcccaagact
//
LOCUS       DASTKS010000756         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_343917, whole genome shotgun sequence.
ACCESSION   DASTKS010000756 DASTKS010000000
VERSION     DASTKS010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343917"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFQ35_15675"
     CDS             complement(<1..896)
                     /locus_tag="VFQ35_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9932143.1"
                     /translation="MKTRLLNWILLATVASAFSAACGDDDDSSGATGGAHQGGKTNTG
                     GAGRGGGGHGGAQAGETSSGGVGEGGATISQGGSTVVPEGGASGASPSGGTNPGTGGT
                     NTGGTTTASGGTNAGGTTTANGGTTTASGGTNSGGTGTGGIMMTGGAGGAEGGSGGNA
                     AGAGGEGGVALDPVAQRGQYLVVTVAQCSSCHTDPTKATMFLGGNPNFTSGVKIPAPN
                     LTPDVTGLGDWTDEEIKRAIRDGIDDEGRQLAPAMPYWLYHNLTDADADAIVAYLRTI
                     PKISNAVGTTNPAATAVTPLSP"
     gene            complement(1301..2554)
                     /locus_tag="VFQ35_15680"
     CDS             complement(1301..2554)
                     /locus_tag="VFQ35_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932144.1"
                     /translation="MPRVVTQLSELEIARLTLAKTPRETDQELAHSLLQVLRVVADAL
                     DVERVGIWEINADWSRMECQALYERTPKRSSSGIVLELSKFPAYARALKERRVITAED
                     ARADPRTRELEASYLSVHDIGAMLDCPIYDNGSVMGILCQEHVGSSRKWTPRDADFAV
                     NAADIVSTLFAQSARTRYEIELRKAREALAEARVMDSLGRMAARVAHDFNNTLMGISL
                     SLSALEHEAELNTASRAALADSRNLVDQGSRLVRSLLSFARTGDSESCSVNLCEATTE
                     MLPRLKRLVGPDISVQAEVPEEAGLVPLDLARAEQLLMNLVLNARDALPGGGTIRLRL
                     HLSEREVELRVSDNGIGMDAETRQKIFEPFFTTKGDRGSGLGLAIVFSVVRGAGGSID
                     VESELGSGATFTLRLPRLGASAAGQ"
     gene            complement(2618..>4141)
                     /locus_tag="VFQ35_15685"
     CDS             complement(2618..>4141)
                     /locus_tag="VFQ35_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET9932145.1"
                     /translation="ERGHRTWTRPYTFYPDFRTGITRAEPVYDAGGAVLAVITADYDV
                     SELSEILSQATRARDSLVLFTTDGVVLGLSGLKAPPRPNSDGRPLSFDVLGRPELRAF
                     YESRGAKGAAPLREYFVGREAFVASEHHLRGVQGLDWRLALILPKETITAAARAQARK
                     ELWLAGAVVLFAVAIACGLSVGIQRLRKARARAEAQSERVMGKLRELGAYTLEEQLGS
                     GGMGEVYRARHRMLARPAAIKLIRTDMPKEEREALELRFDREAKVLSELRSPHTVAIL
                     DYGRTTDDRLFIVMELLTGLPLDKLVEKHGPQPAARVIPILVGVCRSLAEAHAAGIVH
                     RDIKPANLFLCHDAEGVEIVKVLDFGLAKAARKPGLTSEGHVAGTPHFMPPEQALGRE
                     VDGRADLYALGCTAHFLLTGRTVFEEPSDIAVILAHQMKAPPPLAEHTTAWLPDGMEA
                     LIQRCLAKDPEFRPVSAAALARALLSLRIPEEQQLGAETWRDWWLRFENLPGAVAPS"
BASE COUNT          677 a         1351 c         1362 g          751 t
ORIGIN      
        1 ctcggcgaca gcggcgtaac ggcggtcgcg gcggggttcg tcgtgccgac tgcgttgctg
       61 atcttgggga tcgtgcgcag gtacgcgacg atcgcatcgg cgtccgcgtc ggtgaggttg
      121 tgatagagcc aatagggcat cgccggtgcg agctgacggc cttcatcgtc gattccgtcg
      181 cgaatggcgc gcttgatttc ctcgtcggtc caatcgccga gaccggtcac atcgggcgtg
      241 aggttcggcg ctggaatctt cacaccgctc gtgaagttcg ggttgccgcc cagaaacatc
      301 gtcgccttcg tcgggtcggt gtgacacgac gagcactgcg ccaccgtgac gaccagatac
      361 tgtccgcgct gagcgaccgg atccagcgct acaccgcctt cgccgccagc acccgccgcg
      421 tttccgccgc tgccgccctc ggcgccgcca gcaccacccg tcatcatgat accgccggtc
      481 ccggttccgc cgctgttcgt gccgccgctc gccgtggtgg ttccgccatt ggcggtggtg
      541 gttccgccgg cgttcgtgcc gccgctcgcc gtggtggttc cgccggtgtt cgtgccgccg
      601 gtgccggggt tggtgccgcc gctcggggac gcgcctgagg cgccgccttc gggcaccacg
      661 gtacttccac cttgcgagat ggtcgcgccg ccttctccaa cgccaccgct agaagtttct
      721 ccagcttgcg cgccgccatg gccgccgccg ccgcgccctg cgccgccggt gttcgttttg
      781 cccccttggt gagcgccacc cgtggcgccg gaagagtcgt cgtcgtcgcc gcaagcagcc
      841 gaaaaggcgc tcgcaaccgt ggccaacaag atccaattca gcaatcgagt tttcatcaca
      901 cactcgcgtg aaagagcgtt cccctgaacg agaaccccgt atattggtta ttcgcgccgg
      961 gcgcacgtgg tttcgaatca aaatcatcga acgtgcgttt cccgtcgggg aaatgcatct
     1021 gaaacggggc ggcgcggacg accgctagcg gcgccaagcg gcccggagtt tcgccgtcgc
     1081 gagaaccaag agcgagcgag aggcgccacg attcgaggat ccggtcgagg cactcgcgag
     1141 ggagactccg cgggcagtgc cgaagctggc ctaaagtgcg tccatgggcc tgggacattc
     1201 ttgcattggc ctggtcagtg gctttgcgag tgcggcaacg gtcagtgact ccgaacgtgg
     1261 cggaaaacgg ctccgcacaa ggtacccgcg tggggtgagt ttactgcccg gccgcactcg
     1321 cgcccagcct cggaagccgc aacgtgaacg tggcgccgct gccgagctcg ctctccacgt
     1381 cgatgcttcc gcccgcgccg cgcactacgc tgaaaacgat cgcgagtccg agcccggagc
     1441 cacgatcgcc cttggtcgta aagaatggct cgaagatctt ctgtcgggtc tccgcatcca
     1501 tgccgatgcc gttgtccgaa acgcggagct caacctctcg ttcggagagg tgtaaccgga
     1561 gccggatcgt gccgccgccg ggaagggcat cgcgcgcgtt cagcaccaga ttcatcagca
     1621 gctgttccgc gcgcgcgagg tcgagcggca ctaggcctgc ttcttccggt acttccgcct
     1681 gcaccgagat gtccggcccg accaatcgct tcaagcgagg aagcatttcg gtcgtcgctt
     1741 cgcacaggtt caccgagcag ctttcgctgt cgcccgtgcg agcgaaggac aggagcgagc
     1801 gaacgagccg tgagccttga tcgacgaggt tcctcgagtc ggcgagggcg gctcgagacg
     1861 ccgtgttgag ttcagcctcg tgctccagtg cggacaagct gagcgatatg cccatgagcg
     1921 tgttgttgaa atcgtgcgcg acgcgggccg ccattctgcc gagcgagtcc atgacgcgtg
     1981 cctcggcgag cgcctcgcgg gcctttcgga gctcgatttc gtagcgggtc cgggccgact
     2041 gcgcgaacaa cgtcgatacg atgtcagctg cgttcacggc gaagtcggcg tcgcgcgggg
     2101 tccacttgcg cgatgagccc acgtgctcct gacacaaaat tcccatgacg gaaccgttgt
     2161 cgtagatggg gcagtccagc atcgcgccga tatcgtggac gctcagatag ctcgcctcga
     2221 gttcgcgcgt tcgtgggtcg gcgcgcgcgt cttccgccgt gatcacgcgg cgctccttca
     2281 acgctcgagc gtaggcgggg aacttcgaca gttcgagcac gatgccagag gaagagcgct
     2341 tcggagttct ctcgtacagg gcctggcact ccatgcggct ccagtcggcg ttgatttccc
     2401 agatgccgac ccgctcgacg tcgagcgcgt ccgcaaccac tctcagaact tggaggagcg
     2461 agtgcgcgag ctcttggtcg gtttctcgcg gggtcttcgc cagagtgagg cgcgcgatct
     2521 cgagttcgct cagctgggtc accactcgag gcatgttact ccgttggtac ccacgccgga
     2581 ccaggattcg cagtctacga ctgcaagcag gctaatttca ggatggggcg acggctccgg
     2641 gtaggttttc gaagcggagc caccagtcac gccaggtttc ggcgccgagc tgctgctctt
     2701 cggggatgcg cagggagagg agtgcgcgcg cgagcgctgc cgcggagacc ggacgaaact
     2761 ccggatcctt cgcgaggcag cgctggatca aggcctccat cccgtcgggc agccacgcgg
     2821 tcgtgtgctc ggcgagcggc ggcggcgcct tcatttggtg ggcgaggatg accgcgatat
     2881 cgctcggttc ttcgaagacg gtccttcccg tcaagagaaa gtgagccgtg caaccgagcg
     2941 cgtagaggtc ggcgcggcca tcgacctcgc gcccgagcgc ctgctcaggc ggcatgaaat
     3001 gcggcgttcc tgcgacgtgc ccttcgctgg tcaggcccgg cttgcgcgct gccttggcga
     3061 gtccgaaatc gagcactttg acgatctcga cgccctcggc gtcgtggcag agaaacaggt
     3121 tcgccggctt gatgtcgcgg tgaacgatgc cggccgcgtg cgcctccgcg agcgaacggc
     3181 acacgccgac caaaatcgga atgacgcgtg cggccggttg cggcccatgt ttctcgacga
     3241 gcttgtcgag cggcaacccc gtcaaaagct ccatgacgat gaataggcga tcgtcggtgg
     3301 tacggccgta gtccaggatc gccaccgtgt ggggtgagcg cagctcagag agcaccttgg
     3361 cctctcgatc gaagcgcagc tcgagtgcct cacgctcttc cttcggcatg tcggtgcgaa
     3421 tgagcttgat cgccgccgga cgcgccaaca tgcggtgccg cgcgcggtac acttcgccca
     3481 tgccgccgct cccgagctgc tcttcgagag tgtaggcgcc gagctcgcgc agctttccca
     3541 tcactcgttc ggactgcgct tccgcgcgcg cgcgcgcttt gcgtagacgt tggattccaa
     3601 ccgagagccc gcacgcaatc gcgaccgcga agaggactac tgcgccggcc aaccagagct
     3661 ctttgcgggc ctgtgcgcgg gccgcggcgg tgatggtctc tttcggcaaa atcagagcca
     3721 gacgccaatc gagaccttgc acgccgcgca ggtgatgttc actcgccacg aaggcttccc
     3781 ggccgacgaa gtattcgcgg agcggagcgg cgcctttggc tccgcgcgat tcgtaaaacg
     3841 cgcgcagttc gggacgcccg agaacatcga agctcaacgg ccgcccgtcg gagttgggcc
     3901 gcggcggtgc tttcaaccct gaaagtccga gcaccactcc atcggtcgtg aacagaacca
     3961 aggagtcgcg cgcgcgcgtc gcttggctca atatctccga aagctcgctc acgtcgtagt
     4021 ctgccgtaat cacggcgagg acggctccgc ccgcgtcgta cacgggctcg gcgcgcgtga
     4081 tgcccgtgcg aaagtcaggg tagaaggtgt aaggccgcgt ccaggtccgg tgccctcgct
     4141 c
//
LOCUS       DASTKS010000757         3933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1012808, whole genome shotgun sequence.
ACCESSION   DASTKS010000757 DASTKS010000000
VERSION     DASTKS010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012808"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..898)
                     /locus_tag="VFQ35_15690"
     CDS             complement(107..898)
                     /locus_tag="VFQ35_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9932146.1"
                     /translation="MRAKTRKKRPLPETHAIAEAIRPWLAAWHERQGSPTTGPVFPVR
                     RGPRAGESKARSNMSYARRLRRELRKAGVDRHGLHNETPTTKPTVFHTLRGAFATALA
                     RVGANAQLAMSLTGHADSKVHRRYLDQETIRQLPAAVPAIGLVVLPSNENRPKSGAKP
                     ETLASPNSVFSGAGHEARTRDPELGKVDGLEIAQESSGNASARTPGGSTFPVASVTRA
                     VLPVPVSDRVGGAAVWDEVIRAWCRARAQELATEWVRVQSCGSSS"
     gene            complement(985..2172)
                     /locus_tag="VFQ35_15695"
     CDS             complement(985..2172)
                     /locus_tag="VFQ35_15695"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9932147.1"
                     /translation="MGSLSDSTHPLSSEVVPTSLHYSASVHLGFLDGVRGLAAGYVVL
                     HHVMLNVPRSPNAPGWERALRLATGYGHYAVDLFIVLSGYCLMLPVLRSPRQFDSARF
                     LIRRAARILPPYYAAMLFSWALILSFIGHPSGTHWDVSIPVSARDIVYHSLLIHDLSE
                     ASAPKINHAHWSVAVEWKLYFLFPLLLIAKKRFGAVVTVVAATVSSYVLWLVLRRYSL
                     FNPSPWGSSVYYLGLFAFGMLAADLAEPQARSFSPTFRRNINVGVALLSLVVVALSSR
                     YFGRLGDLPLQVVSAGVGAWSAVLLVSLRVRSLPRWLSTTFSNPASEWIGRRSFSLYL
                     THAPILELVYRCLAAARVTGASLILLMLGLATPAAVLISAAFYRLVELPSHQLSRRVT
                     AGA"
     gene            complement(2315..3238)
                     /locus_tag="VFQ35_15700"
     CDS             complement(2315..3238)
                     /locus_tag="VFQ35_15700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9932148.1"
                     /translation="MGELPLFSPRRPLGKTGFAVTRVGIGDLADRSIPIEQCVSTLRR
                     ALDAGLNIVDTAPSYEDGYSEQIVGRALAGRRQGVFLIDKVDHLDRPVLPQVAESLER
                     LGTNSVDLFVFHGVSSLAAYRELMAKGGGWDQLEACRAAGHCRFVGLSSHHPGVLKEA
                     IEAGRSDVLMFPVGPFVDARYVDEILPLTRERGIGSVCFKTFGAGKLLGDTEGYGREL
                     SLRPRGKLSSGGTDQAEPTLPRLGVEECVRYTLTLDPDVALLGMSFPNEQDAALSAAA
                     NFKPLSAAELDAVRSRASEAIRDKGPCWWNP"
     gene            complement(3240..>3933)
                     /locus_tag="VFQ35_15705"
     CDS             complement(3240..>3933)
                     /locus_tag="VFQ35_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932149.1"
                     /translation="ATLTVIPEEFETSRITLDQIADILVASIVKRLAYGRSDGVAVLA
                     EGLVEHLDEKELEKYVPNVERDEHDHIRISEVEFGPIIKTRVSEKLKELGIKITIVSK
                     NIGYELRCTDPIPFDMEYTRDLGYCAAKYLIEGGSGAMVTIQRGCFKPIKFADMLDPQ
                     TGRTRVRMVDTNTEHYKIARRYMLRLRQDDFEDPAEIASYAAVAKLTPEAFKERFEYL
                     VKNEAPPISFQV"
BASE COUNT          733 a         1208 c         1325 g          667 t
ORIGIN      
        1 cgttctgtga ccacgcccac gcgatcattg gctactacac cgtggtgaac cagcggcgtt
       61 ggtttcgaga gtacccgtac gcggtcaggg aagcggacgg gacgagctat gacgatgatc
      121 cgcagctttg aacccgcacc cattcggtcg ccagctcctg tgctctcgcc ctgcaccacg
      181 cacgtatgac ttcatcccac accgcagcgc cgcctactcg atccgaaacg ggcacgggca
      241 aaacggcacg agttacggaa gcgaccggaa acgtacttcc gcccggggtg cgggcgctcg
      301 catttccgct ggattcctgc gcgatctcta agccgtccac cttcccaagc tcggggtcgc
      361 gagttcgagc ctcgtgtccc gctccagaaa agacgctgtt tggtgaagcg agcgtctccg
      421 gtttcgcccc tgacttgggg cgattctcgt ttgaggggag aacgacaaga ccgatcgccg
      481 gcacggcggc gggcagctgc cggatcgtct cctggtcgag gtagcggcga tgcaccttgc
      541 tgtcggcgtg gccggtgagg ctcatggcca gctgtgcgtt cgcgcccacc cgcgccagcg
      601 cggtagcgaa tgcgccgcgc agcgtgtgga acacggtcgg cttggtcgtc ggggtctcgt
      661 tgtggagccc gtgccggtcg acgcccgcct tcctgagctc tcgccgaagg cgtctcgcgt
      721 aggacatgtt cgagcgagcc ttgctctcgc cggcgcgtgg cccgcggcgg accgggaaga
      781 ccgggcccgt cgtcgggctg ccctggcgct cgtgccacgc cgcgagccaa ggccgaatgg
      841 cttcggcgat agcgtgggtc tcaggcagcg gccgcttctt gcgagtcttg gcgcgcacga
      901 aggtgacgct ctcgaagccg ggtgagaact gcgtccagtc gacgtggggg ctgagggtgc
      961 gcgcgagcgt cgtcggtcgc tcgtctaggc gccggcggtg acccgacggc tgagctggtg
     1021 cgaaggtagt tcgacgagtc gatagaaggc tgccgaaatg aggaccgccg cgggcgtggc
     1081 gagcccgagc atgagtagga tgagacttgc tcccgtgacg cgagccgctg cgaggcaacg
     1141 gtacacgagc tcgaggatgg gagcgtgggt gagatagagg ctgaagctac gccgtccgat
     1201 ccactcggag gcggggttac tgaacgtcgt cgatagccaa cggggcagcg agcgcactct
     1261 gagcgagacg agtagcacgg cgctccaggc gccaactcct gcgctgacca cttgcagcgg
     1321 caagtcgccg agcctgccga agtagcgaga agacagggcg accacgacga ggctcaagag
     1381 cgcgacgcca acgttgatgt tgcggcggaa ggtcggcgaa aacgagcgag cctgcggctc
     1441 ggcgaggtcg gcggcgagca tcccgaacgc gaacaacccg aggtagtaca cggagctgcc
     1501 ccaagggctc gggttgaaga gcgagtatct tcggagaacg agccagagca cgtacgacga
     1561 gacagtcgcg gcgacgacgg tcacgacggc gccgaagcgc ttcttcgcga tgaggaggag
     1621 aggaaacaag aaatagagtt tccactccac ggccacagac cagtgggcgt gattgatctt
     1681 gggagccgag gcctcgctga gatcgtgaat gagcagcgag tgataaacga tgtcccgcgc
     1741 cgaaaccgga atggagacat cccagtgggt gccgctcggg tgcccgatga acgatagaat
     1801 gagcgcccaa gaaaagagca tcgccgcgta atacggaggc aaaatgcgag cggcgcgtcg
     1861 gatgaggaag cgggcggagt cgaattgccg gggcgagcgg agcaccggca gcatcaagca
     1921 atagccggat agaacgatga aaaggtcgac cgcgtagtgt ccgtagcccg ttgctagccg
     1981 aagcgcgcgt tcccatcccg gtgcgttcgg tgagcgcggc acgttgagca tcacgtgatg
     2041 caaaacgacg tacccggccg cgagtccccg cacaccgtct agaaacccga ggtggacgga
     2101 cgcgctgtag tggaggctcg tcggcacgac ctcgctcgac aacggatgcg tgctgtcgct
     2161 caagctgccc atggaaaggc cgccaactct gacatggtgc gcagcctagc agcgccatga
     2221 gcgtcgtgtg ccgtcttcgt gccgtgcgaa cgcgaaggtc cgttacgccg ctgcgagatc
     2281 gaggcgaacc ccgtaagctt ttttcagcgc gtcgctacgg attccaccag cacggaccct
     2341 tgtcgcgaat cgcctcgctc gcgcggcttc gaacggcgtc gagttcggcc gctgaaagcg
     2401 gcttaaaatt cgctgcagcg gagagcgcgg cgtcttgctc gttcggaaag ctcatgccga
     2461 ggagcgcgac gtcgggatcc agcgtgagag tgtagcgcac gcactcttcg acgccgagcc
     2521 gcggtagcgt gggctccgcc tgatcggtgc caccggagct cagcttgccg cgcggacgta
     2581 acgagagctc gcgtccgtat ccctctgtat cgccgagcag tttaccagca ccgaacgtct
     2641 tgaagcacac gctgccgatg ccgcgctccc gcgtgagcgg caagatttcg tcgacgtagc
     2701 gcgcgtcgac gaagggtccg accgggaaca tcaaaacgtc ggaccgtcca gcttcgattg
     2761 cctctttcag cacacctggg tggtgagacg agagcccgac gaagcggcaa tgcccggccg
     2821 cgcggcacgc ttcgagctgg tcccagccgc cacctttggc catgagctcg cgatacgcag
     2881 cgagggacga gacaccgtgg aaaacgaaca gatcgaccga attggtgccc aggcgctcga
     2941 ggctctcggc gacctgcggc agcaccggcc gatcgaggtg atcgactttg tcgatgagga
     3001 acacgccctg ccggcgccca gcgagcgctc gcccgacgat ctgttcgctg tagccatctt
     3061 cgtagctcgg cgcggtgtcc acgatgttca gccccgcgtc gagagcgcga cgcagggtgc
     3121 tcacgcattg ctcgatcggg atgcttcgat cggcgagatc tccgataccg acccgggtga
     3181 ccgcgaatcc ggtcttgcca aggggacgac gcggtgaaaa gagcggcagc tcgcccactt
     3241 cacacctgaa acgagatcgg aggcgcctcg ttcttcacca agtactcgaa gcgctccttg
     3301 aaggcctcgg gggtgagctt cgcgaccgcc gcgtagctcg cgatttcggc tggatcctcg
     3361 aagtcgtctt ggcggagacg cagcatgtac cggcgcgcga tcttgtagtg ctcggtgttc
     3421 gtatcgacca tgcgaacgcg cgtgcggccc gtctgtgggt cgagcatgtc ggcgaacttg
     3481 atcggcttga agcagccacg ctggatggtc accatcgcgc ccgagcctcc ctcgatgagg
     3541 tacttggcgg cgcagtagcc gaggtctcgc gtgtactcca tatcgaacgg gatcggatcc
     3601 gtgcagcgca gctcgtagcc gatgttcttc gacacgatcg tgatcttgat cccgagctcc
     3661 ttcagcttct cgctcacgcg cgtcttgatg atgggaccga actcgacctc ggaaatgcgg
     3721 atatggtcgt gctcgtcgcg ctcgacgttg ggcacgtact tctcgagctc cttttcgtcg
     3781 aggtgctcga cgaggccttc cgcgagcacg gcgacgccat cggagcgccc gtacgcgagg
     3841 cgcttcacga ttgaagcaac caggatatcg gcgatctgat cgagcgtgat ccggctggtc
     3901 tcgaactcct cggggatgac ggtgagcgtg gcc
//
LOCUS       DASTKS010000758        11149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_343933, whole genome shotgun sequence.
ACCESSION   DASTKS010000758 DASTKS010000000
VERSION     DASTKS010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343933"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1085)
                     /locus_tag="VFQ35_15710"
     CDS             complement(117..1085)
                     /locus_tag="VFQ35_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9932150.1"
                     /translation="MRLGLRSTVASFAFVLLSSSAFAQGRLAETLTGDAKNEYEAAKI
                     LYADGDFAGSALKFHHAYLLSGDARLLWNEAAAEKNQRHYARVEALIREYLQKGGATS
                     EADKADAQALIETVRAFIADLNVTSNESGANVYVDDVQVALTPLAAPLRIDMGLRRVR
                     VVKPGFKEFSKNVEIVGGGQSSVDAQLVAETHQGTLRVVGSADSTIRVDGKVVGIGQW
                     QGTLPSGVHGVEVTADRKLPYRADSLVQDGELSTVQVTLQDEPSSSPVMLQRTSSNTW
                     LWVAGGAVLAAGLGVGAYFALKPDHQGPPAPIDGTIGSVQLDLFRH"
     gene            complement(1097..2521)
                     /locus_tag="VFQ35_15715"
     CDS             complement(1097..2521)
                     /locus_tag="VFQ35_15715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932151.1"
                     /translation="MPPPSQSPVNPGEILAGKYRVERVLGAGGMGVVVSALHLELDQR
                     VAVKFLLSQIASPEAVARFVREARAAVKIRSEHVARVIDVGRLEDGAPYMVMEYLEGR
                     DLAAQLAHGPLPIEDAVDYVIQAADAMAEAHAEGIVHRDLKPANLFLTWRSDGAPVVK
                     VLDFGISKLQTTDSELSLTRTSAVMGSPVYMSPEQARAVHSVDARTDIWSLGIILFEL
                     VTGRTPFRGNTLPELITAIAMDAPEPLRKLRPDAPEELEVAISRCLEKDRARRFQHVG
                     ELVAALVPVAPPRSRPIFERVLKVTGVIEPVPTARQPNTTSGNAAANPTGTAWGATGP
                     QGGRGRGGLWLGVGGGLVVVGLAAAYIGLRPKEATTASPEPASSPSVVPSVPAPAAVV
                     PEVTPVPMPEAPAASAASSALPAAPARGPGRPPTRGLPGVSTQAPRAPENTPAPEASA
                     QLKPTTPPAAPAPTRKRGLEIDLK"
     gene            2685..4898
                     /locus_tag="VFQ35_15720"
     CDS             2685..4898
                     /locus_tag="VFQ35_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932152.1"
                     /translation="MKQGYRWTFFRAGGFDQVKLESGADLLNLENLDQKLWVALACPS
                     TGLEMDARTLALVDSDKDGRVRAPELIAAVKFAGTRLKNADDLLKGSSSLPLASIDDS
                     TPEGKTLLASARQVLRNLGRPDAEALTCDDFADPSKIFADTPFNGDGVVTELTSELED
                     VRALLREIMDTHGSEQDRSGKPGVSGKQADEFFAEVDAYAAWMAKAKESESSVYPLGA
                     EKTSAAVAAVRALRAKVNDYFGRCRLAAFDPRAALVMNRKEEEYLDVATHDLSLTADE
                     VARFPLAQVAADRPLPLTRGVNPAHAAALAKLREDAIVPLLGQREQLSEKDWLSITEK
                     LAAHDAWLAEKPNQKIEKLGNERILAIHGSNLKQVLYDLIAKDKALEAESASIDQVER
                     LVRYHRDLFKLCENFVNFRDFYDGDEPAIFQCGTLYIDQRACGLCLPVHDAAKHATLA
                     GLAGAYLAYVDCVHKATDEKMQIVTAFTDGDSDNLMVGRNGVFYDRKGRDWDATINKI
                     VDNPISLRQAFWAPYKKFVRMIEEQVAKRAAAADTKAHEHLGQAAEATAQVGNTNVVS
                     AKSQEPTKIDVGAVAALGVAVGAIGTALTAFLGYAGGVLKLGLLATIGAVVGLILLIS
                     TPSLVLAYIKLRKRNLGPILDANGWAVNARARINVPFGTTLTAVAKLPPGSRRDSTEL
                     YAERGLPWKRWVFLGIVLYLAYGWYVGNFDRFLPETVRPPAVLGKFAPEKKAPSK"
     gene            4968..5387
                     /locus_tag="VFQ35_15725"
     CDS             4968..5387
                     /locus_tag="VFQ35_15725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9932153.1"
                     /translation="MKYLHTMVRVSNLEQSVDFFCNKLGLRELKRTHNEKGRFTLVFL
                     SAPGDEHAQVELTHNWDPEELRGGRNFGHLAYEVDDIYATCERLQNSGVVINRPPRDG
                     RMAFIRSPDKISIELLQKGDALPPREPWLSMPNTGEW"
     gene            complement(5464..6759)
                     /locus_tag="VFQ35_15730"
     CDS             complement(5464..6759)
                     /locus_tag="VFQ35_15730"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HET9932154.1"
                     /translation="MLLGAAGTLLLACTSGSDSEDGASTRLSESERSGFVNYRGVRTA
                     FVRRDGALFIEGDIEVGPADLAFGESGDSSTHGDVGRVSQAVRRVGATFRWPNAVVGW
                     NWDTTLVDVDGNGTLDSFTAAQQTAIQQAMTDWSRAVPGLSFQQVTTGDRILFRLDKG
                     CSSPVGRQGGAQIIRLTGGCVSSFSVHHEIAHSLGIFHEQSRADRNSFVTINWANIKG
                     CTATATGPTVAQGCGGCSAASPAPCGCTAADFAATPETCNTWQNFVNTNPSADVFNYD
                     LESVMHYPADAFQKAGFGNTITVIGGGTIGQRIRLSSPDRSKMRVLYPSPSVAPVIFA
                     GTGNQRLCLLNGREDDASVDVVTNSLPGAFLNTNSLTPGTTLSATCTMKSALWAETYT
                     YPNAADISFSGRPASEIETYSANRSVPVLSAGLIPIFAM"
     gene            complement(6859..7248)
                     /locus_tag="VFQ35_15735"
     CDS             complement(6859..7248)
                     /locus_tag="VFQ35_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932155.1"
                     /translation="MQTRHRVSMTLAALGFAGVLAAAPTASANGAVGNALITELALSG
                     NVAWLRFNVALGTSNPPNNTRPACIASGWQDYFMFDHTTAQGKSFLTMLSAAMLAGKR
                     VDASGVTSGTLCTNSTAETLGYLKVYP"
     gene            7430..8380
                     /locus_tag="VFQ35_15740"
     CDS             7430..8380
                     /locus_tag="VFQ35_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9932156.1"
                     /translation="MTNTGVRGVPCAALAGVRMLGDLFEARVVHGWLQPRRSRQAHAF
                     SVQFAPTREESPLCLEVPSLFCGGRGAARMAVEAFSNECPLLRLPAVPPTQRVATPTV
                     HTGIWVPLTYGSHDFGVLRIERDSSVVPGAVSRAGRELLPLISAGVRAELEARDTETE
                     LSVQAALGAKYQRTLLFSRENRRVTWTSWVSCGCESEASTVGIETLLDATISEPRLSQ
                     RRQTAGLRWVELGEYSDGAVAVGLCALEQPPEARLSPRERQVSTLLAEGYAIVNIAAV
                     TGLSEHTVRTYARRVYRKLGVHSRASLVRRLLEQSASNLG"
     gene            8408..9304
                     /locus_tag="VFQ35_15745"
     CDS             8408..9304
                     /locus_tag="VFQ35_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9932157.1"
                     /translation="MLDESCSRESVEFLRKDELPGVEVCEVRHSARRWRGFTSSFEII
                     ASSSWCGEVIYKQCLHTLRPGMIFCAQPGESYATPRVHRAGSRATLSIEPAKFFEYLS
                     AHRLANTELSIRRFTPLSHQLAQRFVKVLAMMRGPASGWELQSGVCELVEALILELVS
                     APRAIAPSPAQAWTVDRIREYSDFEAGTFVDLRTLAAQTGLSRFQVLRAFKRKYGLPP
                     HAYQLCVRISSARKALRAGAAVKDVAATHGFVDQSHFARTFKQLVGVTPANYQRAAPP
                     RNWVATARSARLVVLNDSIADG"
     gene            complement(9312..10445)
                     /locus_tag="VFQ35_15750"
     CDS             complement(9312..10445)
                     /locus_tag="VFQ35_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9932158.1"
                     /translation="MTLRRLLPLFATLGILGVFAWTLFFLYKKSEARPVVYETTHAER
                     RDIIKKTVAPGAIVPRREVAIKPRVSGVIEKLFVNPGDLVKEKALLARIRIIPNVVNL
                     NQAEASVRSAQISLETARKEFDRLTKLKEQGLLNQGEFAQQELAFKLRQEELDSAENN
                     LQLVRVGASKKSGNVSNLVYSTVTGMVLEVPVREGGSVIEANNFNEGTTIASVADMND
                     LIFQGRVDESDVGGIKQGMPVSIAVGALSDQRFDGVLEYIAPKGIEKEGTIQFEVRAA
                     VKLKPEATLRANYSANADIILDRRDNALAVNESAVVYQGGKTYVEVETTPQHFERRAV
                     TLGLSDGIWVEVKSGVDEKTSLKKQDPGDTSARPAASGARPKR"
     gene            complement(10569..>11149)
                     /locus_tag="VFQ35_15755"
     CDS             complement(10569..>11149)
                     /locus_tag="VFQ35_15755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9932159.1"
                     /translation="RVGWFALTTLANAPPEVVEVAAKSALQARHNVHPQDPDAIGSYN
                     VASQIAKVEGLFRGLRAFVWFVGTLTLLAGVLGVSNILLITVKERTRELGIRKALGAT
                     PWAIVSMVLKEAVVLTSLSGYLGLVAGVAALEGLAKVIERLPNAPLNQPEIDLKVALG
                     AMLVLIVSGTLAGVVPARHAARVSPVEALRTE"
BASE COUNT         1963 a         3587 c         3569 g         2030 t
ORIGIN      
        1 cgaggagtga gcaccggagg aaggctgaag cctttcgagg agcggagcga cgaggccgtg
       61 cttcgcagcc gcaggatctt ggctcgcagc agggcgggga acttgggaca ccccctctag
      121 tgcctgaaca ggtcgagctg cacgctgccg attgtgccgt cgatcggcgc gggggggcct
      181 tgatggtcgg gcttcagcgc aaagtatgct cctacgccga ggcccgccgc gagcacggcg
      241 ccgccagcga cccagagcca ggtattgctg ctcgtgcgct ggagcatgac cggcgaggaa
      301 ctgggctcgt cctgcagcgt cacctgcacg gtgctgagct ccccatcctg gacgagtgag
      361 tcggctcgat agggcaattt gcgatctgcc gtgacctcga cgccgtgcac gccgctcggc
      421 aacgtgcctt gccactgacc gatgccgacc acctttccgt cgacgcgaat cgtcgagtcg
      481 gcgctgccaa cgacgcgcag cgtaccttgg tgcgtttcgg ctacgagctg tgcgtcgacg
      541 ctcgactgac caccgcccac gatctcgacg ttcttcgaaa attctttgaa gcccggcttc
      601 accactcgca cacggcgaag ccccatgtcg atgcgtaggg gcgcagcgag tggcgtcaac
      661 gcgacttgca cgtcgtcgac gtaaacgttc gccccgcttt cgttcgacgt cacgttgaga
      721 tccgcgatga acgcgcgcac cgtttcgatc agcgcctggg cgtcggcttt gtccgcctcg
      781 gaagtcgcgc cacccttctg caggtactcg cggatgagcg cttcgacgcg agcgtagtgg
      841 cgttggttct tttccgcggc ggcctcgttc cacaggagcc gcgcatcgcc ggaaaggaga
      901 taggcgtgat ggaacttcag cgccgagccc gcgaagtcgc cgtcggcgta gaggatcttc
      961 gcggcttcgt actcgttctt cgcgtcgccg gtcagcgtct ccgcgagccg tccttgagcg
     1021 aatgcggagg aagacaggag aacgaacgcg aaggaggcaa cagtcgaacg gagaccgagg
     1081 cgcatggcgc cgcagtctac ttgagatcga tttcgagccc gcgttttctt gtgggagccg
     1141 gcgcggcggg tggcgtcgtc ggttttagct gggctgaagc ttccggcgct ggcgtgtttt
     1201 ccggggcgcg cggcgcttgg gtggaaacgc cggggagtcc gcgggtaggc ggtcgacctg
     1261 gccccctggc gggcgctgca ggcagcgctg atgacgcggc gctcgccgcc ggagcctcgg
     1321 gcattggcac tggcgttact tcgggcacga ccgcggccgg agcaggcacc gacgggacaa
     1381 cggagggcga ggaggcgggc tctggcgacg cggtggtcgc ttctttgggc ctgagcccga
     1441 tgtaggcagc tgcaaggcca acgactacca gcccgccgcc gacgccgagc cacaagccgc
     1501 cgcgcccgcg tccgccctgg gggccagtgg cgccccacgc ggtgccggtt gggtttgccg
     1561 ccgcgtttcc cgacgtggtg ttcggctggc gcgccgtcgg caccggctcg atgacgcctg
     1621 ttaccttcag cacgcgctcg aaaatcgggc gactgcgcgg cggagccacg ggcacgagcg
     1681 cggcgacgag ctcgccgaca tgctggaagc gccgcgcgcg atctttctcg aggcagcgcg
     1741 atatcgctac ctcgagctcc tcgggcgcgt cgggcctgag cttgcggagc ggctccggcg
     1801 cgtccatcgc aatcgcggtg atgagctccg gtaacgtatt gcctcgaaac ggcgtacgtc
     1861 ccgtgacgag ctcgaacaga atgattccga gcgaccagat gtcggtgcgc gcatcgacac
     1921 tatgcaccgc gcgcgcttgc tcgggagaca tgtagacggg cgagcccatg accgcgctcg
     1981 tccgggtgag gctcagctcg gaatcggtcg tttggagctt cgaaatcccg aaatccaaaa
     2041 ccttgaccac gggggcgcca tccgaacgcc aggtgagaaa cagattcgca ggcttcaagt
     2101 cgcgatggac gatgccctcc gcgtgcgcct cggccatggc gtcggccgcc tgaatcacgt
     2161 agtcgacggc gtcttcgatc ggcagcggcc cgtgcgcgag ctgcgccgcg agatcacgcc
     2221 cctcgaggta ctccatcacc atgtacggcg cgccgtcctc caggcgcccg acgtcgatca
     2281 ctcgagccac gtgctcgctg cgaatcttca ccgcggcgcg cgcttcacgc acgaagcgcg
     2341 cgaccgcttc cggactcgcg atctgcgata gcaggaattt gaccgcgacg cgctgatcga
     2401 gctcgaggtg aagcgccgaa accacgacgc ccatcccgcc cgcgcccaag actcgctcga
     2461 cgcggtactt gcccgccaag atttcgcctg ggtttaccgg gctctgagag gggggcggca
     2521 tgctcgacga catggcaatc gcggatccgc gacaggctag cacggcccgc ggttcttcgc
     2581 gttcaggtgc gataaagccc agcaatgaac tgcctatgat gaaccgtccg ctcggagtcc
     2641 gagggctctc gcggccgcgg cagcgctgta gtactgcgct ctacatgaag cagggctatc
     2701 ggtggacctt ctttcgggcc ggcggtttcg atcaggtcaa gctggagagc ggagcggacc
     2761 tgctgaatct cgagaacctc gaccagaagc tgtgggtagc gctcgcttgc ccgagcaccg
     2821 gactcgagat ggatgcacgg acgctcgcgc tcgtcgacag cgacaaggat ggtcgcgtgc
     2881 gcgctcccga gctcatcgct gccgtcaagt tcgctggaac gcggctgaaa aacgccgacg
     2941 acctgctcaa gggcagctcc tcgcttccgc ttgcgtcgat cgacgacagc acccccgaag
     3001 gtaagacact cctcgcttcc gctcgccagg ttctgcgcaa cttgggtcgc cccgacgccg
     3061 aggcgctcac ttgcgatgac ttcgcggacc catcgaagat tttcgccgat actccattca
     3121 acggtgacgg cgtcgtcacc gagctcacga gcgagctcga agacgtccgg gctctgctcc
     3181 gcgagatcat ggatacccac ggctccgagc aggaccgctc gggcaagccg ggcgtctccg
     3241 gcaagcaagc ggacgagttc tttgccgagg tcgacgccta cgcggcgtgg atggcgaagg
     3301 cgaaggagag cgaatcctcg gtgtacccgc tcggcgcgga gaagacgagc gccgccgtcg
     3361 cagccgttcg agcgttgcgc gcgaaggtga acgactactt cggacgctgc cggttggctg
     3421 cgttcgatcc gcgcgcggct ctcgtcatga atcgcaaaga ggaagagtac ctcgacgtcg
     3481 ccacccacga cctcagcttg accgccgacg aagttgcccg ctttccgctc gcgcaggtcg
     3541 ccgccgatcg accgctgccg ctcacgcgcg gcgtgaaccc agcccacgcc gcagccctcg
     3601 ccaagctacg cgaagacgcg atcgttccgc tgctgggaca gcgcgagcag ctctccgaaa
     3661 aggattggct ttccatcacc gaaaagctcg ccgcgcacga cgcttggctc gccgaaaagc
     3721 ccaatcaaaa gatcgagaag ctcggcaacg agcgcatcct cgccattcac ggctcgaacc
     3781 tgaagcaggt gctctacgat ttgatcgcga aggacaaggc gctcgaagca gagagcgcga
     3841 gcatcgatca ggtcgagcgc ctcgtgcgct atcaccgtga tctcttcaag ttgtgcgaaa
     3901 acttcgtcaa cttcagagac ttctacgatg gggacgagcc cgcgattttt cagtgcggca
     3961 cgctctacat cgatcagcgt gcctgcgggc tctgcctgcc cgtgcacgac gcggcgaagc
     4021 acgcgacgct cgccgggctc gccggcgcgt acctcgcgta cgtcgattgc gtgcacaagg
     4081 ccaccgacga gaagatgcag atcgtgaccg ccttcaccga cggcgatagc gacaacttga
     4141 tggtcgggcg gaacggcgtc ttttacgacc gcaaggggcg cgactgggac gcgacgatca
     4201 acaagatcgt cgacaacccg atcagcctgc gtcaggcgtt ctgggcaccg tacaaaaagt
     4261 tcgttcgaat gatcgaagag caggtcgcga agcgcgcggc cgccgcagac accaaagctc
     4321 acgagcacct cggccaagcc gccgaggcca ccgcccaagt tggcaacacc aacgtcgtgt
     4381 cagccaaatc ccaagaacca acgaagatcg acgtcggagc cgtagccgcg ttgggagttg
     4441 ccgtgggcgc aatcggtacg gccttgaccg cgttcctcgg ctacgcgggt ggcgttttga
     4501 agctcggcct gctcgcgacc attggcgcgg tcgtcggcct catcttgctc atctcgacgc
     4561 cgagcctcgt gctcgcttac atcaagcttc gaaagcgaaa cctcggtccc atcctcgacg
     4621 caaacggctg ggccgtgaac gcgcgcgcgc gcatcaacgt gccgttcggg acgaccctga
     4681 ccgcggtcgc gaagcttccg ccaggttcgc ggcgtgactc gaccgagctt tacgccgagc
     4741 gcggccttcc gtggaagcgc tgggtcttcc tcggcatcgt gctctacctc gcgtacggct
     4801 ggtacgtggg caacttcgat cgcttcttgc cggaaacggt gcgcccaccg gcggtgctcg
     4861 gcaagttcgc tcccgagaag aaagccccgt cgaaatagcg gcacgtggct tcgagtgagc
     4921 gaatttgcgc ggtttgcgtg gactacgcga gcgcgtaaag ttcgcccatg aaatacctgc
     4981 acacgatggt gcgagtcagc aacctcgagc agtcggtcga cttcttctgc aacaaactcg
     5041 gcctccgcga gctcaagcgc actcacaacg agaaggggcg cttcacgctc gtgttcctat
     5101 cggcgccggg agacgagcac gcccaggtcg agctcactca caattgggat ccagaagagc
     5161 tccgcggcgg acgaaacttc ggccacctcg cgtacgaggt cgacgacatt tacgcgacct
     5221 gcgagcgctt gcagaactcc ggcgtcgtca tcaaccgacc gccgcgcgac gggcgcatgg
     5281 cgttcatccg ctcgcccgac aagatctcga tcgagctctt gcagaagggt gacgcgctcc
     5341 ctccgcgcga accctggcta tcgatgccga acactggtga gtggtgaaga gcgccccctg
     5401 ccgcgtcgaa tctggaactg ggctcttcga gcccgttcca ggctccgatc cgaggcgtga
     5461 gcgctacatc gcgaaaatcg ggatcagtcc tgcgctcaac acggggacgg aacggttcgc
     5521 gctgtacgtc tcgatttcgc tggcagggcg accgctgaag ctgatatcgg ctgcgttcgg
     5581 gtaggtgtag gtttcggccc agagcgcgct cttcatggtg cacgtcgcgc tgagggtcgt
     5641 ccctggggtg agggaattcg tgttgaggaa cgcgcccggc aagctattgg tgacgacatc
     5701 gaccgaagcg tcgtcttcgc gaccgttcag caagcagagc cgttggtttc ccgtacccgc
     5761 gaagatcacg ggcgcgaccg agggcgaggg atagagcact cgcatcttcg agcggtcggg
     5821 cgagctcagc cgaatccgct gcccaatggt gccaccgcca atcaccgtga tggtgttccc
     5881 gaatccggcc ttttggaaag cgtcggccgg atagtgcatt acggactcca gatcgtagtt
     5941 gaacacgtcg gcgctcgggt tcgtgttcac gaagttttgc cacgtgttgc aggtctctgg
     6001 agtcgcagcg aagtctgcgg ccgtacaccc gcacggcgca gggctggccg cgctgcagcc
     6061 gccgcagccc tgggcgacgg ttggacccgt tgcggtcgcc gtgcagccct tgatgttggc
     6121 ccagttgatg gtcacgaagc tgttgcgatc ggcgcgcgac tgctcgtgaa atatgccgag
     6181 cgagtgggcg atctcgtggt gtaccgagaa gctgctcacg caaccgcccg tcaaccgtat
     6241 gatctgggcg ccaccttgcc ggcccactgg gctcgaacag cccttgtcca accggaatag
     6301 gattctgtcg cccgtagtca cttgttggaa actcaggcct ggcaccgccc gggaccaatc
     6361 ggtcatcgcc tgctgaatgg cggtctgctg ggcggccgtg aacgaatcga gggtaccgtt
     6421 gccatcgaca tcgacgagag tggtgtccca gttccagccc accaccgcgt tcggccaccg
     6481 aaaggtcgct ccaaccctcc ggacggcttg gctgacgcga cccacatcgc cgtgagtgga
     6541 ggagtctccg ctttcgccaa acgcgaggtc cgccggcccc acttcgatat cgccttcgat
     6601 gaagagcgcc ccatcgcggc gcacgaacgc cgtccgaacg ccgcgatagt tgacgaaacc
     6661 gctgcgttcg gactccgaca gccgagtgct cgcgccatcc tccgagtccg accccgacgt
     6721 gcacgccaag aggagtgtgc cggcggctcc gaggagcatt ccatatcgta ttcggtcgca
     6781 tgctgcgttg atagaactca tggtattgct cctctgttca catccagagc caggaacgat
     6841 gagacaatcg ggccgcgact acgggtacac cttgagatac cccaacgtct ccgccgtact
     6901 gttcgtgcac agggtcccgg acgtcacgcc cgaagcgtcg acgcgcttgc cggccaacat
     6961 cgccgcgctc agcatggtca agaacgactt cccctgagcg gtcgtatgat cgaacatgaa
     7021 gtagtcttgc cagccactgg cgatacacgc cggtcgcgtg ttgttcgggg gattcgacgt
     7081 gcccagcgcg acgttgaagc gaagccacgc gacattgccc gaaagtgcca gttcagtaat
     7141 cagcgcatta cccacggcgc cattcgcgct cgcggtcggt gccgccgcca gaactcccgc
     7201 aaaaccgagc gccgccagtg tcatcgaaac tctatgtcgc gtttgcatgg gcaatccctt
     7261 tctctctaat agcgaatcgg cgcgtcagct gttttccgtg cttcgcgctc ctgtcgaacg
     7321 acagcgacac tacagctctg gtgtggatcg ccctagtcgc actagtgagt ggcattccgc
     7381 actttcgtac tagtcgttac tcacgctgag ctgttaggtt gccgcgcaca tgacgaacac
     7441 cggcgtgcga ggcgtcccat gtgcggcgtt ggccggtgtc cgcatgttgg gagatctctt
     7501 cgaggctcgg gtcgtgcacg gctggcttca gccccgacgc agccggcaag cgcacgcctt
     7561 ctccgtacag ttcgctccga ctcgagaaga gtcaccgctc tgcctcgagg tgccgagctt
     7621 gttttgcggt ggcagagggg cggcgcggat ggcggtcgaa gcgttcagca atgagtgtcc
     7681 gctgctgcgt ctgccggcag tgccgcccac gcagcgtgtc gccacgccga ccgtacacac
     7741 tggcatttgg gtgccgttga cctacggcag tcacgacttt ggcgtgctgc gcatcgagcg
     7801 cgacagtagc gtcgttccag gtgcggtgtc gcgtgcgggg cgtgagctgc taccgctgat
     7861 tagcgccgga gtacgcgcag agctggaggc gcgcgacacc gagacggagc tttcggtcca
     7921 ggcggcgctc ggtgccaaat accagcgcac gttgctcttc tctcgcgaaa atcgaagggt
     7981 gacgtggacc tcgtgggtga gctgcgggtg cgaatccgaa gcgtccaccg tcggcatcga
     8041 gacattgctc gacgccacca tttccgagcc gcggctgtct cagcggcgcc agacggctgg
     8101 cttgcgctgg gtggagctcg gggaatattc ggacggagcg gtcgcggtcg ggctgtgcgc
     8161 tctcgagcaa ccaccggagg cgcgtctgtc cccgcgtgag cgccaggtca gcaccttgct
     8221 cgcggaaggc tacgccatcg tgaatatcgc cgcggtgacc gggctttcgg agcacacggt
     8281 gcgcacgtac gcccgccgcg tctatcggaa actcggcgtg cattcgcggg cgtcgctggt
     8341 gaggcgcttg ctcgagcaga gcgcctcgaa cctgggctag cccgctcggg cgtgaatcga
     8401 ggtttttatg ttagacgagt cctgttcgcg ggagtcagtg gagtttttgc ggaaggacga
     8461 gttgcccggg gtcgaagtct gtgaggtacg tcactctgcg cggcgctggc ggggcttcac
     8521 cagcagcttc gaaatcatcg cgtcgagtag ctggtgcgga gaagtaatct acaagcaatg
     8581 tctccacacc ttgcgtcccg ggatgatttt ctgcgcacaa ccgggcgaat cgtatgcgac
     8641 gccgcgcgtc catcgcgccg gttcgagagc cacgctgtcg atcgaacccg cgaagttttt
     8701 cgaatacctt tccgcccacc ggctcgcgaa cacggagcta tcgattcgca gattcactcc
     8761 tctctcgcac cagctcgcgc aacgcttcgt aaaggtcttg gccatgatgc gcggccccgc
     8821 gtctggctgg gaactccagt ctggggtgtg cgaattggtg gaggccttga tcctcgagct
     8881 cgtctctgca ccgcgcgcga ttgcgccatc acccgcgcag gcgtggaccg tcgaccgaat
     8941 tcgagagtat tcggatttcg aggccggtac cttcgtggac ctgcggacac ttgccgcgca
     9001 gacgggactc agtcgctttc aagtgcttcg cgcattcaag cgcaagtatg gtttgccccc
     9061 acacgcctac cagctgtgcg tccggatcag ctcggcgcga aaagcgctac gagccggggc
     9121 tgcggtcaag gacgtggccg ccacgcatgg tttcgtagac cagagccact ttgcgcgaac
     9181 tttcaagcag ctggttggag tgacgccggc gaattatcag cgagcagcgc cgccgcgcaa
     9241 ttgggtggcc acggcgcgaa gcgcgcggct cgtcgtcttg aacgactcaa ttgcagacgg
     9301 gtgagggagg ttcagcgttt cggtcgtgcg cccgaagcgg ctgggcgcgc gctcgtgtcg
     9361 cctggatcct gcttcttgag ggacgtcttt tcgtcgacgc cgctcttcac ctcgacccag
     9421 atgccgtccg agaggccgag cgtcacggcg cggcgttcga agtgctgcgg tgtggtctcg
     9481 acttcgacgt aggtcttgcc gccctggtag actaccgcgc tctcgttgac cgcgagcgcg
     9541 ttgtcgcgcc gatcgaggat gatatcggcg ttggcgctgt agttcgctcg taacgtcgcc
     9601 tccggcttca gcttcacggc ggcgcgcacc tcgaactgaa tcgtaccctc tttttcgatc
     9661 cccttcggcg cgatgtactc gagcacacca tcgaagcgct ggtcggacag agccccgacc
     9721 gcgatggaga ccggcatccc ttgcttgata ccgccgacgt ccgattcgtc gacgcgaccc
     9781 tgaaagatga ggtcgttcat gtcggcgacc gaagcgatgg tcgtgccttc gttgaagttg
     9841 ttggcctcga tgacgctacc gccctcgcgt accggcactt cgagcaccat acccgtcacc
     9901 gtcgaataga cgaggttcga gacgttaccg gacttcttcg aggcgccgac gcgcacgagc
     9961 tgcaggttat tctcggcgga gtcgagctcc tcttgacgaa gcttgaaggc gagctcttgc
    10021 tgcgcaaact ctccttgatt gaggaggccc tgctccttca gcttggtgag ccggtcgaac
    10081 tctttgcggg ccgtctcgag gctgatttgc gcggagcgca cgctcgcttc ggcttggttc
    10141 aagttgacca cgttggggat gatccggatc cgtgcgagga gcgccttctc cttcacgaga
    10201 tcgcctgggt tcacgaaaag cttctcgatg acccccgaaa ctcgcggctt gatggcgact
    10261 tcacgccgcg gcacgatggc gcccggagcg accgtctttt tgatgatgtc gcgacgctcg
    10321 gcgtgcgtcg tttcgtacac gaccggtcgc gcctcggact tcttgtagag gaagaacagg
    10381 gtccaggcga atacgcccaa aatgccgagc gtcgcgaaga ggggcagcag tcgacggagc
    10441 gtcatcgtag aaaggtcacc atccccgagg cggcgttttg ttggcgagcc cgcaaccacc
    10501 gcaagccgaa tttcgatagc accgaagcga cgcaagcgca cgggttcgac cgaaagcacg
    10561 cactcgggtc actcggtcct cagcgcctcc actgggctca ctcgcgcggc gtgtcgagcc
    10621 ggtacgacgc cagccaaggt gcccgacacg atgaggacga gcattgcgcc gagcgctacc
    10681 ttcaggtcga tttcaggctg attcaaggga gcgttgggca agcgctcgat caccttggcg
    10741 aggccttcga gcgcggcgac acccgcgacg aggccgagat agcccgaaag cgacgtgagc
    10801 acgaccgcct ccttcaacac catcgagacg atggcccacg gcgtcgcccc gagcgctttg
    10861 cggatcccga gctcgcgcgt tcgctctttg acggtgatga gcaagatgtt gctcaccccg
    10921 agcacccccg ccagcaaggt cagcgtcccc acgaaccaca cgaaagcgcg aagtcccctg
    10981 aaaagaccct cgaccttcgc gatttgcgac gcgacgttgt acgagccgat cgcgtcgggg
    11041 tcttgcgggt gcacgttgtg gcgggcctgc agggcgctct tcgccgccac ctcgacgacc
    11101 tcgggcggcg cgttggcgag cgtcgtcagc gcgaaccatc ccactcggt
//
LOCUS       DASTKS010000759         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1908084, whole genome shotgun sequence.
ACCESSION   DASTKS010000759 DASTKS010000000
VERSION     DASTKS010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908084"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="VFQ35_15760"
     CDS             complement(<1..2201)
                     /locus_tag="VFQ35_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9932160.1"
                     /translation="MLERLLKYALSNRLAAFVLAAVMLGWGIYAYRNLTIEAFPDPTD
                     TQVQVITLFSGQPTEEVERRVSLPLERALNGTPKLARLRSISLFGLSFVTLTFEDGTD
                     PLIARQQVVERMAGAELPEGVSPSLGPMATPIGEIYRYTLDGGKNTDPMTLRTLQEWV
                     VRPRLLQAPGVADVVSYGGLVREIHVQPDPARMSDLGVGLNDVFEALRKASVNATGGY
                     VERGAEMFVIRSIGIFRSIQDLEGVRVGFHQGVPVSVRDVAQVSEGYAPRQGIVTREK
                     NEDAVEGIVLMRRGENPTIVLDGLRERINELNARGLPGEVKIRAYYDRTELVNTTIKT
                     VFKNLAEGAALVVLVLLAFMLSLRAALIVAAVIPLSLAASFLYLHTRGMSANLLSMGA
                     VDFGIIVDGAVILVEHLFHKLSPAPGHPEEHTDVPLATRIFEASKTVAKPTLFSLLII
                     IAAYLPIFSLQRVEGRIFSPLANTVVSALLGALIMTFTLVPVLSLLALRKPKPARESP
                     LLTWAQRLFDPTLRFAMNNAAAVLIFATCALLSAGTLLTRLGSEFLPALNEGALYVTF
                     TLPDNLSLAEGRKLTPQLKSLLHRTPEVTEVLSQLGRPEDGTDPTLPSNLEIFVKLKP
                     LEQWRPQIHNLDDLIAEMAKQLREVPGIEYNFSQPIRDNVAENISGQFGQVALKIYGD
                     DLDQLQAAAEKSMQVASTVPGAADVGIVKAGETPQLAVRLDRAALARYNLDL"
     gene            complement(2214..3404)
                     /locus_tag="VFQ35_15765"
     CDS             complement(2214..3404)
                     /locus_tag="VFQ35_15765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9932161.1"
                     /translation="MTQNQPETISLSTALALNRQKAWTFLAALGIGAIGLLATALFHR
                     APPPENPPAPGMQVGKTDVTLAANAAQWKVLRLTPVQAPAVHWSNAFPARFTVDEALA
                     AKVGTPLAGRVSTVFVVLGQAVKAGQPLFSVTSPDIAGLRAEREKSAVGLDVAKAGYE
                     RVKAMVDAKALPAKDAIESDQQLREAALALRLAETKLRSLKVSSRGDNEFTVVSPRDG
                     VVIEKNVLPSQQVEGGAPLVSVADLSTVRVAADLFEADAAGIVKGTKALVTSPSLPGF
                     QVETEIEMVSSVADPERHTVPVWVRLPNTDGRIRPNAYAEVRLATNPQPGTLEIPTSA
                     LVSDGAHQYVYVQESPGHFVRRPVVAGSSRDDRLLIYQGLKPGEVVVEQGAVLLDNQI
                     ALAN"
     gene            complement(3401..>4405)
                     /locus_tag="VFQ35_15770"
     CDS             complement(3401..>4405)
                     /locus_tag="VFQ35_15770"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9932162.1"
                     /translation="WNVGVSETVELGKRGPRQAAADLRSRAAKSYYTLALSERVGEAR
                     LSMANALHLMRRTATLEESLEDAQRATALERVRYEQKALAGMDYDRLLLELENLSAEV
                     AHSRAEFQSALADCGAVLLGACDLSSAREEDLDTSLPLPATVPAAPDLRARPDVQGNL
                     LESDAARREAVLARRRAIPDLTLRVGYVRDNTAGGVQAVDGLSVGVMMPLPLSDRGQH
                     DAAKALSRAEELDHQRTATLTVAQAELTGLLRRKEALEHTLSVLEKDSLPRAKSVLES
                     TQQAFDHGGISLTDFLLARRSYVSLRLWLLEQRYELFTVRNDIYRVLGLDARGYENAR
                     "
BASE COUNT          736 a         1412 c         1511 g          746 t
ORIGIN      
        1 agatcgaggt tgtatcgggc gagcgcggcg cgatcgaggc gcaccgcgag ctgcggagtc
       61 tcaccggcct tgacgatgcc gacgtcggcg gcgccgggga ccgtgctcgc tacttgcatc
      121 gacttctcgg cggctgcttg cagctgatcg aggtcgtcgc cgtaaatctt gagcgcgact
      181 tgtccgaact gcccggagat gttctcggcg acgttgtcgc ggatgggctg ggagaagttg
      241 tattcgatgc ccggcacctc acggagctgc ttcgccattt cggcgatgag gtcgtcgagg
      301 ttgtgaattt ggggccgcca ttgctcgagc ggcttcaatt tcacgaagat ttcgaggttc
      361 gagggcagcg tcgggtcagt cccgtcttcg gggcgaccga gctgggacag gacctccgtc
      421 acctccgggg tgcgatgcaa gagggacttc agctgcggag tgagcttgcg tccttcggcg
      481 agcgacaagt tgtccggcag cgtgaacgtg acgtacagcg cgccctcgtt cagcgcgggc
      541 aagaactcgg agccgaggcg cgtgagcagc gtgcccgccg acagcagagc gcacgtcgcg
      601 aagatgagca ccgccgcggc gttgttcatc gcgaagcgaa gcgtggggtc gaagaggcgc
      661 tgggcccagg tgagcagcgg cgactcgcgc gccggcttcg gctttcggag cgcgagcagc
      721 gacagtaccg gcaccagcgt gaaggtcatg atcagcgcgc cgagcagggc gctgacgacc
      781 gtattggcga gcggcgagaa aatgcggccc tcgacgcgct gcagcgagaa aatcggcaaa
      841 tacgcggcga tgatgatcag aagcgagaac agcgtcggct tggcgacggt cttcgacgct
      901 tcgaagatgc gcgtcgcgag ggggacgtcg gtgtgctcct cggggtggcc aggcgcaggg
      961 gatagcttgt ggaacaggtg ctccaccaag atcacggcgc cgtccacgat gatgccgaag
     1021 tcgaccgcgc ccatcgagag caggttcgcc gacatgccgc gcgtgtggag gtacaggaag
     1081 gacgccgcga gcgagagcgg aatgacggcc gcgacgatga gcgccgcgcg cagcgagagc
     1141 atgaacgcca gcaacacgag cacgacgagc gccgcgcctt cggccaggtt cttgaacacg
     1201 gtcttgatcg tcgtgttgac gagctccgtg cggtcgtaat acgcgcggat cttcacctcg
     1261 cccggcaaac ctcgcgcgtt cagctcgttg atgcgctcac gcagcccgtc gagcacgatg
     1321 gtcgggtttt cgcctcggcg catcagcacg atgccttcga cggcgtcttc gttcttctcc
     1381 cgggtgacga tgccctgacg gggcgcgtag ccttcgctca cctgcgccac gtcacgaacc
     1441 gagactggca cgccttggtg gaatccaacg cgcacacctt ccagatcttg gatgctgcgg
     1501 aagatgccga tgctgcggat gacgaacatc tccgcgccgc gctcgacgta accgccggtc
     1561 gcgttgacgc tcgctttgcg cagcgcctcg aagacgtcgt tcagcccaac cccgagatcc
     1621 gacatgcgcg ccggatcggg ctgcacgtga atttcacgga cgagcccacc gtacgacacg
     1681 acgtccgcga cacccggcgc ttgcaagagg cgaggccgca ccacccactc ctgcaaggtg
     1741 cggagcgtca tcggatccgt gttttttccg ccgtcgagcg tgtaccggta aatttcaccg
     1801 atcggcgtgg ccatcggccc gagcgacggg ctcactccct ctgggagctc ggcgccggcc
     1861 atgcgctcga ccacctgctg acgcgcgatc agcggatcgg tgccgtcctc gaaggtgagc
     1921 gtcacgaacg acaaaccgaa gagtgagatg ctacgcaggc gcgcgagctt cggcgtgccg
     1981 ttcagcgcgc gctccagagg cagcgatacg cggcgctcca cctcttccgt cggctgcccg
     2041 gagaagagcg tgatgacctg cacctgcgtg tcggtcgggt ccgggaacgc ctcgatcgtg
     2101 aggtttcggt acgcgtagat gccccagccg agcatcaccg cggccagcac gaacgctgcc
     2161 aggcgattcg atagcgcgta tttgagcaga cgttcgagca tccggcagag acctcagttc
     2221 gcgagcgcga tttggttgtc gagcagcacc gcaccttgct cgactacgac ttcacccggc
     2281 ttcaagcctt gatagatcag caggcggtcg tctcgtgacg agccggcgac gacgggacga
     2341 cgcacgaagt gccccgggct ctcttgcacg tagacgtact ggtgcgcgcc gtcggagacc
     2401 agcgcgctcg tcggaatttc gagcgtgccc ggctgcggat tcgtggcgag ccggacctcg
     2461 gcgtacgcgt tcggacgaat gcggccgtcg gtgttcggca ggcgcaccca aaccggcacc
     2521 gtgtgccgct caggatccgc caccgacgag accatttcga tctcggtctc cacctggaag
     2581 ccggggagcg aagggctcgt gacgagcgcc ttggttcctt tcacgattcc ggccgcgtcg
     2641 gcttcgaaga gatccgccgc gacacgcacc gtgctgaggt ccgccacgct gacgagcggc
     2701 gcgccgcctt cgacctgctg cgacggaagc acgttctttt cgatcacgac gccgtcacgc
     2761 ggcgagacga ccgtgaactc gttgtcgccg cgcgatgaca ccttcaagct gcgaagcttg
     2821 gtctccgcca ggcgcagcgc gagcgccgct tcgcgtagct gctggtcgga ctcgatcgcg
     2881 tccttggccg gaagcgcctt cgcgtcgacc atcgccttca ctcgctcgta gccggctttg
     2941 gcaacgtcca ggccgacggc agacttctcg cgttcggcgc gcagcccagc gatgtcgggg
     3001 ctcgtcaccg agaacagcgg ctgccccgct ttcaccgctt gtcccagcac cacgaacacg
     3061 gtgctcacgc ggccggcgag cggagtgccg accttggccg cgagcgcctc gtccacggtg
     3121 aagcgcgctg gaaaggcgtt cgaccagtgc acggcggggg cttgcacggg cgttagccgg
     3181 agcaccttcc attgagcggc gttggccgcg agcgtgacgt cggttttgcc gacctgcatg
     3241 ccgggcgcgg gcggattctc cggcggtggc gcgcggtgga acagcgcggt agcgagcaag
     3301 ccgatcgctc cgatgccaag cgccgccaag aaggtccaag ccttttgccg attgagggcg
     3361 agggcggtgg agagcgaaat cgtttcgggc tgattttggg tcatcgggcg ttctcgtagc
     3421 ctcgggcatc gagcccgagc acgcgataga tgtcgttgcg aacggtgaag agctcgtaac
     3481 gttgttcgag cagccaaagc cgcaggctca cgtacgagcg acgagcgagc aaaaagtcgg
     3541 tgaggctgat accgccgtga tcgaaggctt gctgtgtaga ctcgaggacg ctcttcgcgc
     3601 gcggcaacga gtccttctcg agcacgctga gcgtatgctc gagggcttct ttacgtcgca
     3661 gcaagcccgt cagctccgcc tgggcgacgg tgagcgtggc ggtgcgctgg tgatcgagct
     3721 cttcggcacg cgagagcgcc ttcgccgcgt cgtgctgtcc gcggtccgag agcggcagcg
     3781 gcatcatcac gccgaccgat agcccgtcga cggcctgcac tccgccggcg gtgttgtcgc
     3841 gcacgtagcc cacgcgcagc gtcagatctg gaatcgcgcg tcgccgagcg agcaccgctt
     3901 cgcggcgcgc ggcgtcgctt tcgagcaaat tgccctgcac gtcggggcgg gctcgcagat
     3961 cgggagcggc cggaacggtg gcaggcagcg gcagcgaagt atccaagtct tcttcgcgcg
     4021 cgctcgagag atcgcaggcg ccgagcagca cggccccgca atcggcgagc gcggattgga
     4081 actcggcgcg gctatgcgcg acctctgcgg agaggttttc gagctcgagc agcaggcgat
     4141 cgtagtccat gccggcgagc gccttttgct cgtaccgaac acgctcgagc gcggtcgcgc
     4201 gttgggcgtc ttctagcgac tcctcgagcg tcgcggtgcg ccgcatcaag tgcagcgcgt
     4261 tcgccatcga gagccgggcc tcgccgactc gctccgagag cgcgagcgtg tagtagctct
     4321 tcgcggcgcg ggagcgcagg tcggccgccg cttggcgcgg accgcgcttg ccgagctcga
     4381 ccgtctcgct gacgcccacg ttcca
//
LOCUS       DASTKS010000760         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1237651, whole genome shotgun sequence.
ACCESSION   DASTKS010000760 DASTKS010000000
VERSION     DASTKS010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237651"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFQ35_15775"
     CDS             <1..791
                     /locus_tag="VFQ35_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932163.1"
                     /translation="GGRLDEAQKAVEELDPKSSDVAVVRSVVAYENGDAAELEAALSL
                     LGASRTSPPFAALAAGPAVLQGTRYLDAQKLQALAAPSIPWGELVAADSALATGNLEL
                     AERVLAVHAKSNAAPVHLLRIARLRRYQKRLDDALSASERALSEKPSAPLGIERVLEL
                     VEKERAGEAREFVARHQGALGASAGWLSVVIDMAANQPKLALGRMTQLEPPSADAPVY
                     LRLIAARALVVSNDKRARGYVLDLARRLGKHPDAIAIAVELGKH"
     gene            complement(862..1731)
                     /locus_tag="VFQ35_15780"
     CDS             complement(862..1731)
                     /locus_tag="VFQ35_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932164.1"
                     /translation="MSLLGTHVTLLSCAAALMTANAWAQDAAAPPAPTPAAEPAPVAA
                     PPPVAAPAPPPAQVQPDTAAQGTAPTAPAAEPAAAAPTGPAGWFRIDSDLTNIQLWAG
                     ATHPLADGIGLASDIYLTTGLSSFGEFDLGPAITAGPFTVTPMLGYQIDWAGRRSNSI
                     VPQLYVTGGPDPIYMEFWFQNYENQIFKDDAPNDIYTRLFVNYKLSPFISIGPEIEPW
                     FALNDAGGSRKTGISSFVVGGDVMFFSYGTNNTLQLFLGYETKKSAQTVGSGEDAVDG
                     RKLGGRFSFVHNW"
BASE COUNT          384 a          712 c          763 g          370 t
ORIGIN      
        1 tcggaggtcg cctcgacgag gcccagaaag cggtcgagga gctcgatccg aagtcgagcg
       61 acgtcgcggt cgtgcgcagc gtcgttgcct acgagaacgg tgacgcggcc gagctcgaag
      121 ccgcgctcag cctactcggc gcgtcgcgca cttcgccgcc ctttgcggcg ctcgcggccg
      181 gcccggcggt gctgcaaggc actcgctacc tcgacgcgca aaagctccaa gcgctcgcgg
      241 cgccgtcgat tccgtggggc gagctcgtcg ccgccgacag cgcgctcgcg accggcaact
      301 tggagcttgc cgagcgcgtg ctcgccgtcc acgcgaagtc caacgcggcg ccggtgcacc
      361 tgttgcgcat cgcgcggctg cgccgctacc aaaagcgtct cgacgacgcg ctttctgcga
      421 gcgagcgcgc actctcggaa aagccgagcg cgccgctcgg catcgagcgt gtgctcgagc
      481 tcgtcgagaa ggaacgcgcg ggggaggcgc gagagttcgt ggcccgccac cagggcgcgc
      541 tcggagcgag cgccggctgg ctctcggtcg tgatcgacat ggccgcgaac caaccgaagc
      601 tcgccctcgg ccgcatgacg cagctcgaac cgccgagcgc ggacgcgccg gtctacctgc
      661 ggctcatcgc ggcccgggcg ctcgtggtct ccaacgacaa gcgcgcgcgc ggctacgtcc
      721 tcgatctagc gcgtcgcctc ggcaagcacc cggacgcgat cgcaatcgca gtcgagctcg
      781 gtaagcactg accgcaaaga gaaacgggga gaaccgtggc tctccccgtc gctcaattca
      841 agtctgcgtc tttgcgcgcg ctcaccagtt gtgcacgaag ctgaagcgtc cgccgagctt
      901 gcgcccgtcg acggcgtctt cgccggagcc aaccgtctgc gccgacttct tcgtctcgta
      961 gccgaggaac agctgcagag tgttgttcgt gccgtagctg aagaacatca cgtcgccgcc
     1021 gaccacgaag ctcgagatgc ccgtcttgcg ggagccgcca gcgtcgttca gcgcaaacca
     1081 cggctcgatc tccgggccga tgctgatgaa cggcgagagc ttgtagttca cgaacaggcg
     1141 cgtgtagatg tcgttcggcg cgtcgtcctt gaagatctgg ttttcgtagt tctggaacca
     1201 gaactccatg tagatgggat cgggaccgcc cgttacgtag agctgcggga cgatcgagtt
     1261 cgacctcctg ccggcccagt cgatttggta accgagcatc ggcgtcacgg tgaacggccc
     1321 cgccgtgatg gccgggccca ggtcgaactc gccgaacgat gacagacccg tcgtaagata
     1381 gatgtcggaa gcgaggccaa tgccgtccgc gaggggatgc gtagcgcctg cccagagctg
     1441 aatgttcgtg aggtcggagt cgatacggaa ccatcccgcg gggcccgtgg gagcggctgc
     1501 ggcgggctca gcagcgggcg ccgtcggcgc ggtgccttgc gcagccgtgt cgggctgcac
     1561 ctgtgccggc ggcggggcag gcgcggcgac aggcggtggg gcggcgacgg gggcgggctc
     1621 cgcggcgggc gtcggagcgg gcggtgctgc cgcgtcttgc gcccaggcgt tcgccgtcat
     1681 gagcgcagcg gcgcacgaaa gcagggtcac gtgggtacca agcagggaca tgcgcatctc
     1741 tagatctccg ggggtatcga gaagccgcga tttcaaggcc gcggaagttg ccgccccggg
     1801 aatcagggac ggctggcgga gacctatcgc tctcggagtt gcgcgtggat ttctcaattg
     1861 tttcgtgact tcgacagcga gttccggttg cgaaacatct cagacatcaa cgtcggtttc
     1921 ggcgcgcacg gtgtaagcgc gtttcaacac atcgaattta aagagtaaaa ttggtcatag
     1981 cgtccaaaat tacaggtagg taactgtgcg atgggatccg aagcgttgct tcggcgaaac
     2041 tcgccagaca cgctccgagc cgacaaaatt agtcgaaaaa agattcggca gtcgcgtact
     2101 accccgggcg cccgaccctg agcactgcgc acgtccggtt ctaggtgcgc ttgaaggcgc
     2161 ggctcgggtt catcgacctt gcaccgcgca cgtccggctc taggcgtgct tcaggcgcgg
     2221 ctcgggttc
//
LOCUS       DASTKS010000761         5820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1237743, whole genome shotgun sequence.
ACCESSION   DASTKS010000761 DASTKS010000000
VERSION     DASTKS010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237743"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /gene="recO"
                     /locus_tag="VFQ35_15785"
     CDS             complement(<1..388)
                     /gene="recO"
                     /locus_tag="VFQ35_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9932165.1"
                     /translation="MPERAVASAHIDSEALLLRRVELGEADLVVTLFTRALGRVSALA
                     RGARKSQKRFGGALEPFFTLRVRLEERPHSELFILAEARVSRARLGMLSDLTRLEVAG
                     RALGWIRKAAPPRTPEPETFAHLERVL"
     gene            complement(381..806)
                     /locus_tag="VFQ35_15790"
     CDS             complement(381..806)
                     /locus_tag="VFQ35_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9932166.1"
                     /translation="MPSVGQFLREQREERGMSVEEISRATRVPVSSVERIESDRFDEL
                     PGEVFVRGFLKSYARALGVAGDEVLARYTASRRVAWVTPLPLSSANPKPARGRRFGVA
                     IAFVLLLILFTLALSIVLKPRGDDMPQELSMRASSAQRA"
     gene            complement(819..1631)
                     /locus_tag="VFQ35_15795"
     CDS             complement(819..1631)
                     /locus_tag="VFQ35_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932167.1"
                     /translation="MMSPIARTVSSFIVAASLAVAGCAGQSSGSGARDPVRLAETEYD
                     LARDLWLQRGQLREALEHALKATELDPDNAEAHHLAALLYLDFCARSPSECRLGEAEK
                     HARLAISLRNDLREAKNTLGVILINAHRAREAVEVLRPLSEDILYSTPEKAWGNLGLA
                     YLDAGDIDRAIDALRRSLAAQPLFCVGSYRLGLAYEKKGDLRAAAEALTRALETEAPE
                     CSRLQDALAVRARVRGRLDQVEEQKADLRRCVELSPSSETGKNCRSILEKLK"
     gene            complement(1628..2326)
                     /locus_tag="VFQ35_15800"
     CDS             complement(1628..2326)
                     /locus_tag="VFQ35_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9932168.1"
                     /translation="MTSELDERERLLSRYGRRFDAGSIIYREGEAAAQAFLVQEGRVR
                     LFKPVGAMERSLRVVRPGEVFGESALLSGSTRPATAIALDTVVTLAFDAGGFDQILVL
                     APEVGTRVAQQLVRRLRDAEDQIELLMIRDAQSKVVVALLKLAQNRAGTDARVPALEV
                     TPLELSAQMGLDVDSVKRIVLGLRESGYVRIQDERIEIPDVEALRELHGLLGVKEQLR
                     GGARDVRSRPSQRG"
     gene            complement(2340..3011)
                     /gene="tsaB"
                     /locus_tag="VFQ35_15805"
     CDS             complement(2340..3011)
                     /gene="tsaB"
                     /locus_tag="VFQ35_15805"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9932169.1"
                     /translation="MRILALETSSSRGSVALTEGATLVATEEHSEPAAHAERMLPLVG
                     RALERAGWARSSLDRIAVGIGPGSFVGLRVGIALAEGLSLGLGRPVVGVESLSAILWA
                     MQLEHVGPRAALVDARRGEVFCAAEDANGRSLLEACAIGRASVSEVLAGLGDGLAVAG
                     EVTATLNLPQKWLRGPLLDLPHAHAIAALASARSVTDEPVLPHYVRGPGAVKPNLPPS
                     PLTPR"
     gene            3132..4343
                     /locus_tag="VFQ35_15810"
     CDS             3132..4343
                     /locus_tag="VFQ35_15810"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9932170.1"
                     /translation="MPSILPRFDYRVDYAIEKDQGRERATYEDAALVAPELAVFAVAD
                     GMGGHAAGEVASRMAVDEVRHALSGRTAQRAVEAYLQRADLSSRREVFAQLRAAVERA
                     NERVRNDAAENEEHRGMGTTLDVVWLARDHAFVAHAGDGRVYLARARAVLQVTQDHTT
                     AELARSDGQPAPTGYRSGITNAVGLSDAIAVDMAFVDLTKGDRLLVCSDGVHSQIDGE
                     GELTELLRSGDAKIAARGLIARAAEGGRDNATAIVVEIGERFVRRGDRDRGLDSADLE
                     RARQSPLLVDLPESFALSALTAAVEVEVSANDRVPRVVANDLVAYVVLDGVVHYPTSG
                     RRMGAGALVFPESLVGVTGISEAPVAEQTARLLRVRADDFAEVCSDPRLAAQLYRRLA
                     QHLARLTSRTP"
     gene            4522..4599
                     /locus_tag="VFQ35_15815"
     tRNA            4522..4599
                     /locus_tag="VFQ35_15815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4557..4559,aa:Pro,seq:ggg)
     gene            complement(4693..5466)
                     /locus_tag="VFQ35_15820"
     CDS             complement(4693..5466)
                     /locus_tag="VFQ35_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932171.1"
                     /translation="MSSPHLALPLLLLAQPAPVTTPGSTPDLTTQPASAGVPEAAPPA
                     VVPAPPPLPATAGAPTPTSAPAPTAAPPPPVSAAPAALIPPATAAPAAPQTPAPSSTP
                     TPTDSLVPAPSASTAGLRRTSLAGFAEGALGLADDGFYNQLVGVRLDYRPSDRLALGF
                     ALSYANLKGKNGRAHNVLPAAMLEWRIPASDRVAIPVRFFSGYLPNNGPWLKAALGLS
                     HRLGERSRITLEAIAPALWIVHDASVGSFDASLEYAVDL"
     gene            5548..>5820
                     /locus_tag="VFQ35_15825"
     CDS             5548..>5820
                     /locus_tag="VFQ35_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932172.1"
                     /translation="MSGSTAIFEDQAHEFDGAFTRRARCFAPGVGGDFGVGADVCEAE
                     ELARSFEEAWRLFSLAMAAQAIAAMRAEIVAAAVLLGRGFRAVTFDA"
BASE COUNT          962 a         1858 c         2039 g          961 t
ORIGIN      
        1 ccaagacgcg ctcgaggtgc gcaaacgtct cgggctccgg ggtgcgtggt ggcgcggcct
       61 tgcgaatcca tccgagcgcg cgcccggcca cttccaagcg agtgaggtca ctcaacatac
      121 cgagtcgcgc gcggctgacg cgggcctcgg cgagaatgaa gagctcgctg tgggggcgct
      181 cttccaagcg aacgcgcaac gtgaagaagg gctcgagagc tcccccgaag cgcttctgac
      241 tcttgcgagc gccgcgcgcg agcgccgaca cgcgtccgag cgcgcgcgtg aacaacgtga
      301 ccaccaagtc ggcttctccg agctcgacgc gccggagaag caacgcctcg ctgtcgatgt
      361 gggcgctcgc caccgcgcgc tcaggcacgc tgggcggagg acgcgcgcat cgacagctct
      421 tgcggcatgt cgtcgccgcg cggcttcagc acgatgctga gcgccagcgt gaacagaatc
      481 aaaagcagca cgaacgcgat cgcgacaccg aagcgccggc cacgtgcagg tttcgggttc
      541 gcgctcgaca aaggcaatgg ggttacccat gccactcgtc tgctcgcagt gtaacgagcg
      601 agcacttcgt caccggcgac gccgagggcg cgcgcgtacg acttcagaaa gccacgtacg
      661 aagacttctc cggggagctc gtcgaagcga tccgactcga tgcgctccac gctcgacacc
      721 ggcacacgcg tcgcgcgcga gatttcctcg acgctcattc cgcgttcttc gcgctgctcg
      781 cgcagaaact ggccgacgct cggcatgggg cagtgagact acttcagctt ttccaatatg
      841 gagcggcaat tcttgccggt ttcgctgctc ggcgagagct ccacgcagcg gcggaggtcg
      901 gccttctgtt cctcgacttg atcgaggcga ccgcgcacgc gcgcgcgtac cgccagcgcg
      961 tcttgcagcc ggctacactc gggggcttcg gtttccagtg cacgcgtaag cgcttccgcg
     1021 gctgcgcgca agtcaccttt tttctcgtag gccaggccga ggcggtaact gcctacgcaa
     1081 aacagcggct gagctgccaa gctgcggcgg agcgcgtcga tggctcggtc gatgtcacct
     1141 gcgtccaggt acgcaaggcc gaggttgccc cacgcctttt cgggcgtcga atagaggatg
     1201 tcctcggaaa ggggccgcag cacttcgacc gcctcgcgag cgcgatgggc gttgatgagt
     1261 atcaccccca aggtgttctt cgcttcccgc aggtcgtttc gcagcgaaat cgcgaggcgc
     1321 gcgtgttttt cagcctctcc gagccgacat tcgctcggac tccgcgcgca gaagtcgagg
     1381 tagagcagcg ccgcgaggtg gtgcgcctcg gcgttgtcgg ggtcgagttc ggtcgctttc
     1441 agcgcgtgct cgagtgcttc gcggagttga cctcgctgga gccaaagatc tcgagccaag
     1501 tcgtattcgg tttcggccaa acggacgggg tcacgggccc ccgaaccgga tgattggcca
     1561 gcgcatcccg ccacggcgag actcgcggcg acgatgaagg acgatacggt gcgtgcgatg
     1621 ggtgacatca tcctcgttgc gaaggtcgcg aacggacgtc tcgcgctccg ccacgtagct
     1681 gttccttcac gccgagcagg ccgtgaagtt cacgcaaagc ctccacgtcc gggatctcga
     1741 tgcgctcgtc ttggatgcgc acgtacccgg attcacgcag accgagcacg atgcgcttca
     1801 ccgagtccac gtcgaggccc atttgcgccg acaactcgag cggcgtcact tcgagggccg
     1861 gcactcgcgc gtccgtcccg gcgcggtttt gcgcgagctt caacagcgcg accaccactt
     1921 tggattgcgc gtcgcggatc atcaggagct cgatctgatc ttctgcgtcg cgcagacgtc
     1981 gaacgagctg ttgagccact ctcgtgccga cctcgggagc gagcaccagg atttgatcga
     2041 agccgccggc atcgaacgcg agcgtcacga ccgtatcgag cgcgatcgca gtcgccggac
     2101 gggtcgagcc gctgagtagc gccgactcgc cgaacacctc accgggtcgc accactcgca
     2161 ggcttcgctc cattgcgccc accggtttga acaaccgcac gcgcccctct tgaacgagga
     2221 acgcttgtgc cgcggcttct ccctctcgat aaatgatgct gccggcgtcg aagcggcgcc
     2281 cataacgaga aaggaggcgt tcgcgctcgt ctagctcgga cgtcatcgcc acttggactc
     2341 taccttggag tgagcggaga tggcggtaaa ttgggcttca ccgcgccggg gccgcgcacg
     2401 tagtggggta acactggttc gtcggtgacc gagcgagcgc tcgcgagcgc agcgatcgcg
     2461 tgggcgtgtg gcaaatcgag cagcgggccg cgcagccatt tttgcgggag attgagcgtg
     2521 gcggtgactt cccctgccac cgccagtcca tcgccgagcc ccgcgagcac ctcgctcacg
     2581 cttgctcggc cgatcgcgca cgcttcgagc agcgaccgcc cattcgcgtc ctcggcagcg
     2641 cagaagacct caccgcgccg agcatcgacg agcgcagcgc gtggaccaac gtgctcgagt
     2701 tgcatcgccc acaatatcgc actcaaggac tcaactccta cgaccggccg accgagtccg
     2761 agcgagagtc cttctgcgag ggcgatgccg acgcgcagcc cgacgaacga gcccggcccg
     2821 atgccgacgg cgatgcgatc gaggctcgat cgggcccacc ctgcgcgctc gagagcgcga
     2881 ccgacgaggg gcagcatgcg ctccgcgtgc gcggcaggtt cgctgtgctc ctccgtggcg
     2941 accaacgtcg ccccctcggt gagcgcgacg ctgccgcgtg agctcgaggt ttcgagggcg
     3001 aggattcgca tggcggggcc gcggtttagc gcgcacaacg ccacgtccgc aaccgagaag
     3061 cccgaaatag cacgctcggg acggcgcctt tttttgggga aacgcgagcg cgcctggggc
     3121 tatgctgtgc cgtgccgtcg attttgccgc gcttcgacta tcgcgtcgac tacgcgatcg
     3181 agaaggacca gggacgggag cgagcgacgt acgaagacgc agcgctcgtc gcgccagaac
     3241 tcgcggtgtt tgccgtagcc gatgggatgg gcggtcacgc cgcgggagaa gtcgcttctc
     3301 gcatggccgt cgacgaggtg cggcacgcgc tttctggacg caccgcccag cgcgccgtcg
     3361 aagcgtatct gcagcgggcg gatctttcct cgcggcggga agttttcgcg cagctgcgcg
     3421 cggccgtcga acgcgcgaac gagcgcgtcc gcaacgacgc ggcggagaac gaagagcacc
     3481 gggggatggg aacgacgctg gacgtggtct ggctcgctcg cgaccacgcc ttcgtagcgc
     3541 acgcagggga cggccgtgtc tacctggctc gcgcccgcgc cgtgctgcaa gtgactcaag
     3601 accacaccac cgcggagctc gcgcgctcgg atggccagcc tgctcccacg gggtaccgct
     3661 cggggatcac gaacgcggtc ggcttgagcg acgcgatcgc ggtcgacatg gcattcgtgg
     3721 atctgaccaa gggcgaccgg ctcctcgtct gcagcgacgg cgtccactct cagatagatg
     3781 gggaaggcga gctcacggag ctcctgcgct cgggggacgc gaaaatcgcg gctcgcgggc
     3841 tcatcgcgcg cgccgccgag ggcgggcgcg acaacgcaac cgcgatcgtc gtcgaaatcg
     3901 gcgaacgctt cgtgcggcgc ggtgatcgcg atcgcggcct cgactccgcc gacctcgaac
     3961 gagcgcgaca aagtcccttg ctcgtggatc tgcccgagtc gttcgcgttg agcgcgctca
     4021 cggccgcggt ggaggtcgag gtgagcgcga acgatcgcgt gccgcgcgtc gtcgcgaacg
     4081 atctcgtcgc gtacgtcgtg ctcgatggcg tcgttcatta tccgacgagc ggccgccgga
     4141 tgggcgccgg tgcgctcgtg ttcccggagt cattggtcgg tgtcaccggg atcagcgagg
     4201 cgcccgtcgc cgagcagacc gcgcgcttgc tgcgcgttcg cgccgacgac ttcgccgaag
     4261 tctgctcgga tccgcgcctt gcggctcagc tttaccggcg tctcgcccag cacctcgcgc
     4321 ggctcacttc tcgaacgccg taacccggcg cgaccggacc cgagccagcg gtgttgacgg
     4381 ctctcgggcg aattctttgc cgcaaggcgt cgggctccgc acaaagctgt tgcaatcctt
     4441 gctggaagga ctacgttgcg cgggcttcta acggtcggcc ctagctaaaa acatagcgaa
     4501 aatccgactg ttggtcggga tcggggcgta gcgcagcctg gttagcgcac cagactgggg
     4561 gtctgggggt cgcaggttcg aatcctgccg ccccgaccaa tgttgagcag agagagcgcg
     4621 gggaggtagc ctcgaaagag gctccggggt cgcgctcgag ctccttcact cgagctcttc
     4681 acttgaagtc cttcaaaggt cgactgcgta ctcgagcgac gcgtcgaaag agcctacgga
     4741 cgcgtcgtgc acgatccaga gcgcgggcgc gatcgcttcg agcgtgatgc gggagcgttc
     4801 tccgagtcgg tgactcagcc cgagcgcggc tttcagccag ggtccgttgt tcgggaggta
     4861 gccgctgaaa aagcgaacgg ggatggcgac gcggtcggac gcaggaatgc gccactcgag
     4921 catggcggcg ggaagcacgt tgtgcgcgcg tccattcttg cctttgaggt tcgcgtagct
     4981 gagcgcgaag ccgagcgcga ggcggtctga ggggcgatag tcgaggcgga cgccaacgag
     5041 ctggttgtag aagccgtcgt cggccaagcc gagcgcgcct tctgcaaatc cggcgaggct
     5101 cgttcgccgc agcccggcgg tgctcgcact cggtgccgga accagtgagt ccgttggtgt
     5161 tggcgtcgag ctcggtgcag gagtttgcgg ggcagcgggc gcggcagtgg caggagggat
     5221 cagtgcggcg ggcgcggcgc tgacaggagg agggggtgcg gcggtaggtg cgggcgcgct
     5281 cgtcggggta ggagcgccag ccgtcgccgg cagcggcggg ggtgctggaa cgaccgcagg
     5341 tggggccgcc tcgggaactc cagcggatgc aggttgggtc gtcaggtcgg gtgtggatcc
     5401 gggcgtcgtc actggcgctg gttgcgcgag gaggagcagg gggagggcga gatgagggct
     5461 cgacacgtga cctcagcgag tggaagcagg gggagggttc agcacttgcg cggcgcgctc
     5521 gaggagaaac tcggcgtaac gcgggttgtg agcggcagca ccgcgatctt cgaggaccag
     5581 gcgcacgagt tcgatggcgc gttcacgcgg agagctcgct gctttgctcc gggcgtgggc
     5641 ggggacttcg gagtgggggc cgacgtttgc gaagccgagg agctcgcgcg ctcgttcgaa
     5701 gaggcgtggc gattgttctc gctcgccatg gcagcgcagg caatcgctgc gatgcgcgcg
     5761 gaaatcgtgg ccgcggccgt gctcctcggg cgcggctttc gtgcggtgac attcgacgca
//
LOCUS       DASTKS010000762         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_790139, whole genome shotgun sequence.
ACCESSION   DASTKS010000762 DASTKS010000000
VERSION     DASTKS010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790139"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFQ35_15830"
     CDS             <1..447
                     /locus_tag="VFQ35_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932173.1"
                     /translation="VRHWLVGFEGRADGYRSLIGSDPETTLELAVLGGRRFDVGDMAL
                     DVSAGPGIAIEGVALSSARTAQASSGNVMARAPEPVEQRSGALPRLLVGARLGFSPRS
                     VFRTFIGVDAELGPVRTSDSSEPNLSATHLPAYLVGLALGATVGSP"
     gene            444..1028
                     /locus_tag="VFQ35_15835"
     CDS             444..1028
                     /locus_tag="VFQ35_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9932174.1"
                     /translation="MTVRGAKHASSRGGQETSDPELMQQLARGQLNALGDLYDRYQAP
                     VRSFIARATRDAHDVDDLVHATFLAAAQCAERYDGRASCRPWLIGIAARHLRRRRQTF
                     ARLVSMLTALQGTRPLESDPRPSLQARTDIERALALISEPKRITLLMAEVEGLTCAEI
                     AEALSIPIGTVWTRLHAARRELRAALGAPGDAKP"
     gene            1025..2161
                     /locus_tag="VFQ35_15840"
     CDS             1025..2161
                     /locus_tag="VFQ35_15840"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9932175.1"
                     /translation="MTTATKPACARLFEAEALRDGRLSGAEVDRYHAHLKVCSTCASE
                     VEALQALANALRSSAPPSPINELHVRRERTRLLAAFDARLVAAPAGLHRNRRRNAVAA
                     LAVLSALAVTLAFVFSPSRRVAPVATSPAVSPEPVIVQADSKTQWSRRTESDLETITL
                     QSGVLSIRVDHDAAAPPRRLLVILPDGELEDIGTAFSVTADAGHTTQVSVRAGSVVLR
                     LHGKPPLALGAGDSWSPVPVYDTPAISAPARASNTSAIPAPAPASAPAASSSSPPPVP
                     SARSPRATPALPRTAASAPDAFTDFRIAMSRLNSGDNAAAAAQFAAFVHQHPNDSRAE
                     DAAYLCVLAWQRAGNSSATRRAATEYLARYPRGFRRAEVESLAR"
BASE COUNT          409 a          987 c          759 g          464 t
ORIGIN      
        1 gtccgccatt ggctcgtcgg attcgagggt cgcgcggacg gctatcgatc tctaatcggt
       61 agcgatccag aaacgacgct cgaactcgcc gtattgggcg gccgccgctt cgacgtcggt
      121 gacatggcgc tcgacgtcag cgcggggcct ggcatcgcaa tcgagggtgt tgctctttcg
      181 tcggcccgaa cggcgcaggc gagtagcggc aacgtgatgg cacgcgcgcc agaacccgtc
      241 gagcagcgat cgggcgcgct gcccaggctg ctcgtcggtg cgcgcttggg attcagtccc
      301 cgctccgtct ttcgcacctt cattggagtc gacgcagagc tcggcccggt gcgcacgtcc
      361 gattcgtccg agcccaacct ctccgcgacc cacttgcccg cctacctcgt aggtctcgcg
      421 cttggcgcca ccgtgggtag tccatgaccg tgcgcggcgc caaacacgca tcttcgcgcg
      481 gcggtcagga aacctccgat ccggagttga tgcagcaact ggcgcgaggt cagctgaacg
      541 cgctcggcga cctctacgat cgctatcagg ctccggtgcg cagtttcatc gcccgcgcca
      601 cgcgcgacgc gcatgacgtc gacgacttgg tgcacgcaac gttcctcgcg gctgctcaat
      661 gtgccgaacg ctatgatggc cgcgcgtcgt gccgtccttg gctcatcggc atcgcggcac
      721 gccacctacg tcggcgccgc caaaccttcg cgcgcttggt ttcgatgttg accgcactcc
      781 aaggcacgcg accgctggag agcgacccgc gcccgagtct ccaagcccga actgacatcg
      841 agcgcgccct cgctctcatc agcgagccca agcgcatcac gctcctcatg gctgaagtcg
      901 aagggctcac gtgcgccgag atcgccgagg cgctctccat ccccatcggc accgtgtgga
      961 cccgtcttca tgccgcccgt cgcgagctgc gcgcggccct cggcgcgcca ggagacgcga
     1021 aaccgtgacg acggccacca agcccgcgtg cgcccggctc ttcgaggccg aagccttgcg
     1081 cgacggccga ctcagcggcg ccgaagtcga tcgctaccac gcccacctca aagtctgctc
     1141 gacgtgcgct tccgaggtag aagccctgca agccctcgcg aacgccctcc gctcgtcagc
     1201 gcccccgtca ccaatcaacg aacttcacgt gcgccgcgaa cgcacccgtc tactcgctgc
     1261 gttcgacgcg cgcctcgtcg cagcaccggc cggccttcac cgaaatcgac ggcgcaacgc
     1321 ggtcgccgct cttgcggtcc tctccgcgct ggcagtcacg ctcgcgttcg tattctcgcc
     1381 ctcgcgccgg gtcgcgcccg tcgcgacttc gccggccgtg tcgcccgagc ccgttattgt
     1441 ccaagcggac agcaaaaccc aatggtcgcg acgaaccgaa agtgacctcg agacaatcac
     1501 tctccaaagt ggcgtgctct cgatccgggt cgatcacgac gcggcggctc caccacgccg
     1561 cctcctcgtc atcctccccg acggcgagct cgaagacatc ggcaccgcct tctccgtcac
     1621 cgccgacgct ggccacacca cccaagtttc cgtgcgcgca ggcagcgtcg ttctacgcct
     1681 ccatggcaaa ccgccactcg cactcggcgc cggcgactcc tggagccccg ttcccgttta
     1741 cgacacgcca gccatttccg cgcccgcgcg cgcttccaat acgtcagcca ttcccgcgcc
     1801 cgcgcctgct tccgcgcccg ctgcgagttc gtcatctcca ccgcctgtac ccagcgcacg
     1861 ctccccgcga gccacacccg ctttgccccg caccgcagca tcggcgcccg acgctttcac
     1921 cgactttcga atcgccatgt cccggctcaa cagcggcgac aatgcggcag ccgccgccca
     1981 atttgcggcc ttcgtccacc aacacccaaa cgactcacgc gcagaagatg ccgcttacct
     2041 ctgcgtgctc gcctggcagc gcgcgggcaa ttcgagcgcc acgaggcggg ccgccactga
     2101 atacctcgct cgctatccgc gtggctttcg tcgcgcggag gtggaatcat tggcgcgtta
     2161 gcgctggcgg ttgcggcgcg ctgatgccgt tgagtcggcg ctcggatgct cggcctcggc
     2221 ccggcccctg cctcgctggc tagctactgt ttccaggcgt cgccgcactc gcgtggggtc
     2281 acgtcagcgc ccccccgtaa catcttgtgt cttactgtcg caacgccggt actccgcggc
     2341 aatttgccgt agacagacca gccttctccg aaggctgcgc ccgcgttcca tgggagcgcc
     2401 gtaattgcaa tggtcggcat tgcggcttaa gtgcggcaga atgtagagtt gggcggaccg
     2461 catggcgaac gagggtctcg actttggtcc gaatcatcga gcggttgctg atctactcct
     2521 cgagtatcgt tcgtcctccc cctaccgaaa tctgggcgag ccaacgtccc tcgacgcttc
     2581 gctcacccga gtagatttct ccttcgtgat cgagcatcg
//
LOCUS       DASTKS010000763         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1237885, whole genome shotgun sequence.
ACCESSION   DASTKS010000763 DASTKS010000000
VERSION     DASTKS010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237885"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFQ35_15845"
     CDS             complement(<1..676)
                     /locus_tag="VFQ35_15845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9932176.1"
                     /translation="MKVSLRHTLYSTAFLRAVSTSATGLFLGIHLAEQGHTPTETGAV
                     ISAGLAGTALSAALVTFFGDRFGKRAWLTGITLLSCVGTISLLFVQSTSLLCVAAFAG
                     MLNGAGRDRGAALILEQAALPTLATAEQRTSVIARYTLLQDVGHALGALLPSAFVKLP
                     LALPASASSELTLVSIAGLGLVSLALYATRGRALDPAEALPKKPLSARSRAILFRISA
                     LFAVDSI"
     gene            1130..2014
                     /locus_tag="VFQ35_15850"
     CDS             1130..2014
                     /locus_tag="VFQ35_15850"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HET9932177.1"
                     /translation="MNSAPNALSAPIDEKAVAEATGVETPERSADGVVKASFPRKDVE
                     VAVDGAKLPPFMGLTSWAAFAPGRKGVADAMVMGDLVLFEDEVNAVLSTLLDHGLEVT
                     ALHNHFFYDTPHVYFMHIGGEGSVTKLGAGVRAAMDAIAAIRKKTPKPGHTFGKGALP
                     VKSTIDATKLEAILGVKGQAKDGMFKATMGRKATASCGCNVGKTMGVNTWAAFFGSDE
                     NAVVDGDFAMAEGELQSVLRTLRAGGIDIVAIHHHMSGESPRVLFLHYWGRGKAADLA
                     VTVKKALDLTAWDRPEAT"
     gene            2043..3059
                     /locus_tag="VFQ35_15855"
     CDS             2043..3059
                     /locus_tag="VFQ35_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932178.1"
                     /translation="MDGYARKLRFSLCALLLGCAKGSHAGGIKLPDTPPGIGFDDLRY
                     SPNLHRVLVPSGRSGRLNLIDPDTLAVTSIEGFSTKAEYSGGHDDGPTSVEEARGLLY
                     VTDRSSQKLVVVDSATRRARGNVALSAEPDYVRFVSATNELWVTEPAADQIEILKLRS
                     DGLPVPSATVHVENGPESLVIDASRGRAYTHRWQKTTLAVDVTTHEIVAEWANGCVAS
                     RGIALDEQRGWLFSGCSEGTVSVLDVAHAGKLLSQLERGSGFDVIGYNARLGHVYLAG
                     GACSCLVILGVNPQGQLSFLERASAPSSTHCAVADDAGNAWVCDPDAGSILRFVDHHA
                     STLD"
     gene            3062..3931
                     /locus_tag="VFQ35_15860"
     CDS             3062..3931
                     /locus_tag="VFQ35_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932179.1"
                     /translation="MNPAAFLRHFGARTLLGSALGLCVLCSGREARAYRPFDGTDADV
                     ADVGVFELELGPAQFYRRGDANYVLAPSTVLNFGIARDLELVVDFKQVIASRASQSEA
                     RVRWLDTDVLLKWLMRRGSLQDGQGLSVALEAGPLVPEFRGEAGFGAQANLIVSARAR
                     FGTLHFNEEAALSRLGRVELFSSIIAEGPESLVVRPVAELFVERELEARSTTYSALFG
                     TIWSAAETLSVDGALRLASEERVRALEVRVGFTWAESLWSRSVAAPQNAHASRRSLSA
                     YSAPLSSPVGGRL"
     gene            complement(3889..>4741)
                     /locus_tag="VFQ35_15865"
     CDS             complement(3889..>4741)
                     /locus_tag="VFQ35_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9932180.1"
                     /translation="WVVHLWVEGFFEVFATVVIAFLFARLGLIRVATAGRSTVLASTI
                     FLGGGVIGTLHHLYFSGTPTFVLALGSVFSALEVVPLLFVGFEAWENLRLSRATPWVQ
                     NYKWPIYCFVAVAFWNMVGAGLFGFMINPPIALYFMQGLNTTPVHGHTALFGVYGMLG
                     IGLMLFCLRALRPSEVWRENLIKPAFWLINGGLLAMVLLSVLPVGLLQTKASVEVGYW
                     YARSEEFLHSGPMSTLRWMRVLGDTAFALGAILLVLFVAGLGTGRSYRARGAQSLPPT
                     GELSGAE"
BASE COUNT          946 a         1509 c         1523 g          763 t
ORIGIN      
        1 cgatgctatc gacggcaaag agcgcggaaa tgcggaagag gattgcgcga ctccgcgccg
       61 aaagcggctt tttcgggagt gcctcggccg gatcgagcgc gcggccccga gtagcgtaga
      121 gcgcaagcga aacgaggccg agcccggcga tgctgacgag cgtgagctcc gaggacgcac
      181 tcgcgggaag cgcgagcgga agtttcacga acgcgctcgg caagagcgcg cctagagcat
      241 gaccgacatc ctgcaataac gtgtacctgg caatgacgct cgtgcgttgt tcggcggtcg
      301 cgagcgtcgg gagcgcggct tgctccaaga tcaacgccgc gccgcgatct ctcccggccc
      361 cgttgagcat gccggcgaag gcggcgacgc acaaaagcga agtcgactga acgaacagca
      421 agctaatggt tcccacgcac gagaggagcg tgatgccagt gagccacgcg cgtttaccga
      481 agcggtcccc gaagaacgtc acgagcgcgg ccgacagcgc ggttccagcc agccccgcac
      541 ttatcaccgc accggtctcg gtcggagtgt gcccctgctc cgcgaggtga atgcccagaa
      601 acaagcctgt ggcactcgtg ctgactgcgc gcaaaaaggc agtcgagtag agggtatgac
      661 ggaggctcac cttcacgacg ccttaacgag cgatagccgg cgttgcactc gtgacgcccg
      721 cgtgatagcg ccgaagtgcg cgggagcgtt gctgcgctct gcccagcgaa agattcttcc
      781 aagaggcaca gggagcgaca gcccgcgttg gtcgagccga ggcgcgacca tgcagctcgc
      841 cgccgccacg accgcgcctg agcctggagc tcgaggcgga gagatcttgc acgcccatcc
      901 gcgcggaacg cattcgaagc gagagagccc aatgatgacg cgccgaatct ctagttcgct
      961 ggtccaagtt tcactactat gcatcgcggc cggctgcgcg gggcagcaat ctcgaccagc
     1021 gccgcccact tcctcggatg ccgcacctgc tcggccgccc accaccgagg cgccgtcgcc
     1081 gagcgccgag ctccccgtca gcgccgcccc cgcaacgccg ccgcccgccg tgaactcggc
     1141 acccaacgcg ctttcggcac cgatcgacga gaaagccgtc gccgaggcca ccggtgtcga
     1201 aacgccggag aggagcgcgg atggcgtagt gaaagcttct ttcccccgca aagacgtcga
     1261 agtggcggtg gatggcgcca agttaccacc gttcatgggg ctgacatcgt gggccgcatt
     1321 cgcgccaggg agaaagggag tcgctgacgc gatggtgatg ggtgacctcg tgctgttcga
     1381 ggacgaggtc aacgccgtgc tctcgacgct gctcgaccat gggctcgagg tgaccgcgct
     1441 ccacaaccac ttcttctacg acacgccgca cgtgtacttc atgcacatcg ggggcgaagg
     1501 aagcgtgacg aagcttggcg ccggtgttcg cgccgccatg gacgctatcg ccgcgattag
     1561 aaagaagaca ccgaagcctg gacacacctt tggaaaggga gcattgcctg tcaagagcac
     1621 gatcgacgcc accaaactcg aggcgattct cggcgtaaaa ggtcaagcca aagacggcat
     1681 gttcaaagca accatgggcc gaaaagctac cgcgagctgc ggctgcaacg tcggcaaaac
     1741 catgggcgtg aacacttggg cggcgttctt cggtagtgac gagaacgcgg tggtagacgg
     1801 cgacttcgcc atggcagagg gcgagcttca gagcgtgctg aggactttga gagcgggcgg
     1861 catcgacatc gtcgccatcc atcaccacat gagcggtgag tctccgcgcg tccttttctt
     1921 gcactattgg gggcgaggaa aggccgccga cctcgcggtg acggtgaaga aggctctcga
     1981 cctcaccgcc tgggatcggc cggaagcgac ctaaggccga cctcgaccag gagtgattca
     2041 ccgtggacgg atatgctcga aagttgcgct tttcgctctg cgcgctgctt ctcggttgcg
     2101 cgaagggctc tcacgccgga ggcataaagc ttcctgacac accgcccggc atcggcttcg
     2161 acgacttacg ctattcgccg aatctccacc gtgtgttggt gccttcggga cgaagcgggc
     2221 gactgaacct gatcgacccc gacacgctcg cggtgacttc gatcgaagga ttttcgacga
     2281 aggccgaata cagcggcggc cacgacgacg gcccgacttc ggtcgaagag gcacgcggtc
     2341 tgctttacgt gacggatcgc agcagccaaa agctcgtcgt cgtagattct gcgacgcgca
     2401 gagcgcgcgg caacgtcgcg ctttcggccg agccagacta cgtgcgcttc gtttccgcga
     2461 cgaacgaact ctgggtaacc gaacccgctg cagatcaaat cgagatcctg aagctgagaa
     2521 gcgatggcct ccccgttccc agcgcgaccg ttcacgtcga aaatggtccc gagtcgctcg
     2581 tgatcgacgc gagccgcggg cgcgcttaca cccatcgctg gcagaagacg acgctggccg
     2641 tagacgtaac gacgcacgaa atcgtggcgg agtgggcgaa cggctgcgtc gcctcgcgcg
     2701 gcatcgccct cgacgagcag cggggctggc tcttttcagg ctgcagtgaa gggacggtct
     2761 cggtcctcga cgtggcgcac gcggggaagc tcctctccca gctcgagcgc gggtcgggct
     2821 tcgacgtgat cgggtacaac gctcggctcg ggcacgtgta cctcgcgggc ggcgcctgca
     2881 gttgcctcgt gatcctcgga gtgaacccgc agggccagct gagctttctg gaacgagcga
     2941 gcgcaccgtc gagcacgcat tgcgccgtag ccgacgacgc tggaaacgcc tgggtttgcg
     3001 acccagatgc cggaagcatc ttgcgtttcg tcgatcacca cgcttcgacg ctggattgag
     3061 cgtgaaccca gccgcgttcc ttcgccattt cggtgcgcgc accttgctcg gctcggccct
     3121 cggcctgtgc gtgctctgct ccggcaggga ggctcgggcc tatcggccct tcgacgggac
     3181 ggacgcggac gtggcggacg tgggggtatt cgagctcgag ctcggtccag cacagttcta
     3241 tcgacgaggc gacgccaact acgtgctggc cccttcgacc gtcctcaatt tcggaatcgc
     3301 ccgcgatctg gagctggtcg tcgacttcaa gcaggtgatc gcgagccgcg cgagccagag
     3361 cgaagcgcgc gtacgctggc tggacaccga cgtgctgctc aagtggttga tgcggcgggg
     3421 cagcctacaa gacggacaag ggctcagcgt cgcgctcgag gctggtcctc tcgtccccga
     3481 atttcgaggc gaagccggct tcggcgctca agcgaacctc atcgtttcag cccgcgcacg
     3541 cttcggaacg ctgcacttca acgaagaggc agcgctctcg cgtctgggtc gcgtagagct
     3601 gttcagcagc atcatcgcgg aaggacccga gtcgctcgtc gttaggccgg tcgcggagtt
     3661 gttcgtcgag cgtgagctcg aagcgcgatc gacgacctac tcggctctct tcggaaccat
     3721 ctggtccgcg gccgagaccc tgagcgtgga tggggctctt cgtttggcga gcgaagagcg
     3781 agtccgcgca ctagaagttc gcgttggatt cacctgggca gagtcgctgt ggtcgcgctc
     3841 cgtcgccgca ccgcaaaatg cacacgcctc gcggcgctca ctctcggcct actcggcgcc
     3901 gctgagctcg ccggtcgggg gcaggctttg agctccgcgc gcgcgatacg accggccagt
     3961 gccgaggccg gcgacgaaca acacgagcaa aatggctccc aacgcgaagg cggtgtcacc
     4021 gagcacgcgc atccagcgca aagtgctcat tggaccgctg tgaaggaact cttcgctgcg
     4081 ggcgtaccag tagccgacct cgacggaggc cttggtttgc agcaaaccca ccggcaatac
     4141 gctgaggaga accatcgcga gcagaccgcc gttgatgagc caaaacgcag gctttatcag
     4201 gttctcgcgc cagacctcac ttgggcgcaa tgcgcgcaga cagaacagca tgagcccgat
     4261 ccccaacatc ccgtatacgc cgaagagagc cgtgtggcca tgcacgggcg tagtgttcag
     4321 gccctgcatg aaatagaggg cgatgggcgg gtttatcatg aacccgaaca gccccgcgcc
     4381 aaccatgttc caaaacgcta ccgcaacgaa gcagtaaatc ggccacttgt agttctgcac
     4441 ccagggcgtg gcgcgagata ggcgcaagtt ctcccaagcc tcgaagccga cgaacaggag
     4501 gggtaccacc tcgagcgcgc tgaacacgga gcccagcgcg agcacgaagg tcggggttcc
     4561 ggaaaaatac aaatggtgca atgtcccgat gactccaccg ccgaggaaga tcgtcgacgc
     4621 gagcacggta ctacgccccg ccgtcgcgac tcgaattaga cccaatcggg cgaacaggaa
     4681 cgcgatcacg accgtcgcaa atacctcgaa gaagccttcg acccacaggt gcacgaccca
     4741 c
//
LOCUS       DASTKS010000764         6303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1013599, whole genome shotgun sequence.
ACCESSION   DASTKS010000764 DASTKS010000000
VERSION     DASTKS010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013599"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            613..1080
                     /locus_tag="VFQ35_15870"
     CDS             613..1080
                     /locus_tag="VFQ35_15870"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET9932181.1"
                     /translation="MFGFLFGAICLGGLASMLFHRPHHHHYGHYGHCGRGFGSRWGRR
                     HFGINMAFDRLDTTPGQEKAILAALDELQESARGVRAKVVASRGDVADALRAEHFDSE
                     RVNTVVARHAEDFATLGNALSATLGKIHEALDPEQRKRLARWLDSGPAFSHFG"
     gene            1096..1299
                     /locus_tag="VFQ35_15875"
     CDS             1096..1299
                     /locus_tag="VFQ35_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932182.1"
                     /translation="MRRKFWAVLLGIGAVAGFASGFRSLHYMGHGPCACGWAGRHANF
                     EAHVADVCTEAVERAQRARGNGH"
     gene            1489..2169
                     /locus_tag="VFQ35_15880"
     CDS             1489..2169
                     /locus_tag="VFQ35_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9932183.1"
                     /translation="MGLRILVVDDDAKLFELLKSYFEQNDVSAISAPDGPSALTLLDT
                     ESVDAVLLDVMMPGMDGLTVLRKLRDKSQVPVIMLTAKGDETDRVVGLELGADDYLPK
                     PFAPRELLARLRAVLRRTQSDLLGRRMQVSGVTLDLDARSVERDGVPVTLTGLEFDIL
                     VALLARAGRVVPRSALLELAGRSDVVVNDRTVDVHVSHLRKKLADDPPRLIRTVRGVG
                     YVFAKDSV"
     gene            2169..3482
                     /locus_tag="VFQ35_15885"
     CDS             2169..3482
                     /locus_tag="VFQ35_15885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9932184.1"
                     /translation="MRAFRRWHRRWHEQHGGRLWQRRRPQLRRQLFAWLVLTIFSTGV
                     AVFLVLRVAGDRRQAFDLRRAEGFVAEHFALTWDDPALRRAAAESLSRTIQVDVLVRD
                     AAGRAMTEVGSCVAPDHSISVRRGTAALGRVDICVKNKGFNRAVSLLAFVTAFLVLWK
                     AASALARFLTRPLSQLIGVTREIGAGNLKSRVRLHRAHRGELGFLADAINDMAERIER
                     QLTEQRELLAAVSHEVRSPLARLRISSELLRENPRDARALASIESEVDEIDTLVGKLL
                     ANSRLDFGTLTRAPLDAASVGRTALARQSLPLDRLADESNGARFSADPTLIARALDNL
                     LDNAIAHGGGLSRLVVRRARPGEHAQAENSVVFEAWDEGPGFDKTAQTRAFEAFFRGA
                     NAASDGRASLGLGLALVQRIAAAHGGRAWAENLPNGGARVCFSAG"
     gene            complement(3568..5538)
                     /locus_tag="VFQ35_15890"
     CDS             complement(3568..5538)
                     /locus_tag="VFQ35_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET9932185.1"
                     /translation="MRGEQRINITALTGLLSALSGVACSSSFDSPPPEAAPFGAGGSS
                     SDIGLGIGGSGRVTDTRAVEKAKTPPPPITGGTLLALSDGKRALVADPDRDRIWIVDY
                     QAGSLSTPIVLEQGAQPTRAVEDDAGQAYVVLRGTGDIMSVDLATATAVERRHVCIAA
                     RGISLAPERDKLLVSCAEGRLVELPFSGSGQKETVIPTDARDVVVQAGRVLVSRFRAA
                     ELLEVTAQGVQPTPLPKFGGVSARLGGASIIDFEPGVAWRTALGPSGHSVVVVHQRAT
                     TTEIQLPPPETTDQPGPTVGPSQGGPGTESAYAGDSSGCGGIVQSAVTVMQDGVTVTS
                     PQLAGVVLPVDVAVSSDGYIAVASAGSADSLLRTKSVSSAVQFLTVGSFDRNINGDCV
                     KGAPVPELANEQITSVAFEPGDTGRALVLARDPFRLFVVQGFRGGVGLLQSVVSIPGD
                     RVTDTGHEIFHRDAGGGIACASCHPEGTDDGRTWNFLPIGGRRTQPIDVGLSGTAPFH
                     WDGDLPTVGSLMSEVFVRRMGGFPESPERINAIESWVFSLRPRAAVRAADDPAAVRGQ
                     ALFSSAEVGCATCHAGSKFTSATSFDVGTGGKFQVPSLLGVAQRLPLMHTGCAHTLRD
                     RFDASCGGTAHGNTSQLDESQLSDLIAYLESI"
     gene            complement(5659..>6303)
                     /locus_tag="VFQ35_15895"
     CDS             complement(5659..>6303)
                     /locus_tag="VFQ35_15895"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9932186.1"
                     /translation="WDTAWTKHLQQQALTERWVVQPLWDETPPPPGMERIVIRPTLAF
                     GDGAHVTTRLAAQAVERFCLALPGASVLDVGTGSGILTLLAALSGAKTAHGVDVDSVA
                     LGAARENAELNGIAQLVSFADAAATAASGYDLVVANLEPRALLADAERIARRARDARE
                     LILTGFLCAQADDVLAKFEEFGFRKLASVEEDEFCLLVLGRNTSAPLSVVPPAG"
BASE COUNT         1076 a         2080 c         2107 g         1040 t
ORIGIN      
        1 gagttccgcg agctcgacgc gcagtgcgtc caagctggcc gaatgagact tctccgcctc
       61 tctcagcatc cgttccgcgc gcgcgcggtc gcactcagct cgcatctgag ctgccttcgc
      121 ttcgacgatt tctcgcccag agcgggaaac cttcgattcg agctcgatca accgcgctcg
      181 ttccccgggg gtgagcgggc gcgcgctcga cggcaccgcg ggcgcgcctg gaatagaggc
      241 aggcgcgggg aacggcggcg gcaccaagct gggcctcgcg tcgctcgcgg cgccctcggc
      301 cgagtccgca gcagcgggtg gggcactggt agccgcgctt ggccggagcg gaggaaccgt
      361 atgcctagga gcggggctcg aggcggtacc atcgggccgg ggaggcgaaa atacgggccg
      421 cgagattcga ggcagtgcag ccagaggctt ttcgccttgt ccaggctctg agtcgcgttt
      481 ctcgtcaccc atcgaggacg aattggagct taacacttct tcacccggga ccgcgaactg
      541 ccccaacacg cgggaccaaa actcctttcg tcccgagcgg caccccgccg ggcacatcga
      601 aaggatctac ggatgttcgg ttttctattc ggcgcaattt gcctcggcgg gcttgcttcc
      661 atgctgtttc accgccccca ccaccatcac tacgggcact atggccactg cgggcgcggg
      721 ttcgggagcc gctggggtcg gcgtcatttc ggcatcaata tggcgttcga tcgcttggac
      781 acgacgccag ggcaggaaaa ggccatttta gccgctctcg acgagctgca ggagtccgcc
      841 cgcggagttc gcgcgaaggt cgtcgcttcc cgcggtgacg tcgctgacgc gctccgcgcc
      901 gagcacttcg acagcgagcg cgtcaatacc gtcgtcgctc gtcacgccga ggactttgcg
      961 acgctcggaa acgcgctgag cgcgacgctc ggcaagatcc acgaggcgct cgaccccgag
     1021 cagcgcaagc gcctcgcgcg ctggctggat tcagggcctg cgttctcgca ctttggatga
     1081 gaaaggaggt tcaccatgag acggaagttt tgggcagttc tcttgggcat cggcgcagtc
     1141 gcgggctttg cctcggggtt tcgcagcttg cactacatgg gccacggacc gtgcgcgtgc
     1201 gggtgggcgg ggcgtcacgc gaatttcgaa gcgcacgtcg ccgacgtgtg caccgaggcc
     1261 gtggagcgcg cacaacgagc gcgcggaaac ggccactgat gccgctcccc gtacggttga
     1321 cttcggcccg ggagaaagct ccgggccgga gtgcccctgg aaagtcgctg gccgaggcgc
     1381 cccgggtcgc gtacacccgc gacgcgttgc tccatcggac gagacctggc gagaggtacg
     1441 ctctcgtttg caattcaccc gagctcgctc ggatgtaagg atgacccaat gggcctcagg
     1501 atcctcgtcg tggacgatga cgccaagctg ttcgagctcc tgaagagcta cttcgaacag
     1561 aacgacgtga gcgcgatttc ggcgcccgac ggaccgagtg cgctcacgtt gctcgacacg
     1621 gagagcgtcg acgcagtgct gctcgacgta atgatgccgg gcatggatgg gctcacggtg
     1681 ctgcgcaagt tgcgcgacaa gagccaggtt ccggtgatca tgctcaccgc caaaggcgac
     1741 gagacggatc gcgtcgtggg cctcgagctc ggagcggacg actatcttcc gaagccgttc
     1801 gctccgcggg agctcttggc tcggctccgg gccgtgcttc ggcgcaccca gtcggatctt
     1861 ttgggccgca ggatgcaggt ctccggggtc acgctcgatt tggacgcgcg cagtgtcgaa
     1921 cgcgacggtg ttcccgtgac gctcaccggg ctcgagttcg acatcctggt agcgctcctt
     1981 gcgcgagccg ggcgcgtcgt gcctcgcagc gctttgctcg agctcgcagg gcgctccgac
     2041 gtcgtcgtca acgatcgcac ggtcgacgtg cacgtctcgc acttgcgcaa gaagctcgcc
     2101 gacgacccac cgcggctgat tcgaactgtg cgcggcgtag gctacgtgtt cgcgaaagat
     2161 tcggtgtgat gcgagcgttt cggcgctggc accggcgctg gcacgagcag catggtggtc
     2221 ggctctggca gcgccgccga cctcaattgc gacggcagct gtttgcgtgg ctggtgctca
     2281 ccatcttcag caccggtgtc gcggtgttcc tcgtgctccg ggtcgccggc gaccgccggc
     2341 aggcgttcga cctacgccgc gccgaaggct tcgtcgccga gcacttcgcg ttgacctggg
     2401 acgatccggc gttgcgccgt gccgcggccg agagcctctc gcgaacgatt caagtcgacg
     2461 tcctcgtgcg cgacgcggcg gggcgagcaa tgaccgaggt gggaagctgc gtcgcgccgg
     2521 atcacagcat ctcggttcga aggggcacag cggcgctcgg acgcgtcgat atttgcgtaa
     2581 agaacaaggg cttcaaccgc gcggtgtcac tgctcgcgtt cgtgaccgca tttctggttc
     2641 tatggaaagc ggcgtcggcg ctcgcgcgtt ttctcacccg cccgctctcg cagctaatcg
     2701 gggtgacacg tgaaataggc gccggtaacc tgaagagccg tgtgcgattg catcgcgccc
     2761 accgaggcga gctcgggttc ctggccgatg cgatcaacga catggcagag cgcatcgagc
     2821 gtcagctgac cgaacagcgc gagctcctcg cagccgtgtc ccacgaagtg cgcagtcccc
     2881 tcgcccgctt gcgcatctcc agcgagctct tgcgcgaaaa tccgcgagac gctcgggccc
     2941 tggcttcgat cgagagtgag gtcgacgaga tcgacacgct ggttggcaag ctgctcgcca
     3001 actctcgcct cgatttcggg acgctgaccc gagcgccgct cgacgccgcc agcgtgggcc
     3061 gtaccgcgct cgcgcgacag agcctgcccc tcgatcgcct cgcggacgag agcaacggag
     3121 ctcgcttttc ggctgacccg acgctgattg cgcgagcgct cgacaaccta ctcgacaacg
     3181 cgatcgccca cggcggaggg ctctcgcggc tcgtcgtgcg ccgggcccgc cccggggagc
     3241 acgcgcaagc cgaaaactcg gtcgtcttcg aggcttggga cgaaggacct gggttcgaca
     3301 agaccgctca gacgagggcc ttcgaagcct tttttcgggg agcgaacgcg gcgagcgatg
     3361 gtcgcgcttc gctcgggctc gggctcgcgt tggtacagcg catcgcggcc gcgcacggag
     3421 gtcgagcctg ggccgaaaac ctgccgaacg gcggcgcgcg agtctgcttc agcgcgggtt
     3481 gatcgaggaa cgcgccgcgc aagtcgaacg gaatcggcgg cccgcgtgag cgaagccgcc
     3541 gaaccgatgc gtcatctcgt cagctactca gatcgactcg aggtaagcaa tcagatccga
     3601 aagctgactc tcgtccagtt gcgacgtgtt gccgtgcgcg gtgccaccgc aggaggcatc
     3661 gaagcggtcg cggagcgtgt gagcacaccc ggtgtgcatg agaggcaggc gctgcgcgac
     3721 gccgagcaac gaaggcacct gaaacttccc gcccgtaccc acgtcgaagc tcgtcgcgct
     3781 cgtgaacttc gagcccgcgt ggcacgtcgc gcagccaacc tcggcggagc tgaacagcgc
     3841 ctggccgcgg accgcagcgg gatcgtcggc ggcgcgcact gccgctcgcg gacgcaaaga
     3901 gaacacccag ctctcgatcg cgttgatgcg ctccgggctc tcgggaaagc cgcccatgcg
     3961 ccgaacgaag acctcgctca tcaagctgcc aacggtaggc agatcgccgt cccaatggaa
     4021 cggcgcggtg ccagagaggc cgacgtcgat tggctgagtg cggcgtccgc cgattggcaa
     4081 gaagttccag gtccgtccgt cgtcggtgcc ttctgggtgg caagacgcgc acgcgatgcc
     4141 gccgccagcg tcgcgatgga agatctcgtg gccggtgtcg gtcacgcgat ctccagggat
     4201 ggagacgacg ctctgaagga gcccgacccc tccgcggaac ccctggacga cgaacagcct
     4261 gaacgggtcg cgcgccaaaa ccagggcgcg gccggtgtct cccggttcga acgccaccga
     4321 ggtgatctgc tcgttggcaa gctccggcac cggcgcaccc ttgacgcaat cgccgttgat
     4381 gtttcgatcg aatgacccga cggtcagaaa ctgcaccgca ctgctcaccg acttggtgcg
     4441 cagcaacgaa tctgccgaac ccgcgctcgc gacggcgatg taaccgtcgc tcgagacggc
     4501 gacatcgacg ggcaagacga cgcctgcgag ctgcgggctc gtcaccgtta cgccgtcttg
     4561 catcaccgta acggcggact gcacgatacc gccgcagccg gacgaatcgc cggcatacgc
     4621 gctctccgtt ccggggccgc cttgcgaagg tccaaccgtc gggcctggct ggtccgtcgt
     4681 ctcgggaggg ggcagctgga tctcggtggt ggttgcgcgt tggtgcacga cgacgacgct
     4741 gtggccgctc gggcccagcg cggttcgcca agcgacgccc ggctcgaagt cgatgatcga
     4801 cgcgccgccg agccgcgcgg aaaccccgcc gaacttgggc aacggggtcg gctggacccc
     4861 ttgggctgtc acctcgagga gctcagccgc gcggaagcga gagaccagga cgcgaccggc
     4921 ttggacgacg acgtcacggg cgtcggtagg aatcactgtt tccttctgac ccgaaccgct
     4981 gaatggaagc tcgacgaggc ggccctcggc gcagctgacg agcagcttgt cccgctcggg
     5041 agcgagcgag atgcccctcg ccgcaatgca aacgtgccga cgttccaccg cggtcgcagt
     5101 cgccaagtcg accgacatga tgtcgcccgt gccgcgaaga acgacgtagg cctggcccgc
     5161 gtcgtcctct actgcgcgcg tgggttgtgc tccctgctcg agcacgatcg gcgtggagag
     5221 gctgcccgcc tgatagtcga caatccaaat gcgatcgcga tcgggatccg cgacgagagc
     5281 gcgcttgcca tccgacaagg cgagtagcgt tccgcccgtt atgggcggcg gcggcgtctt
     5341 tgccttttcg acagcccgag tgtcggtgac acggccgctc ccaccgatgc ccaagccaat
     5401 gtcactcgaa ctgccgccgg ccccgaatgg agctgcttcc ggcgggggcg agtcgaacga
     5461 gctgctgcac gccactcccg agagggcact cagcagaccc gtgagcgccg tgatgttgat
     5521 tcgttgttcg ccacgcatgc cacacactcc ccgcgaagcg gaatcccgcg cgacccaagt
     5581 gcttcgctct tcgcagcatg acggcagtca tagagggtct caaggttaaa gagccgatga
     5641 cgcatcgtgt cagattcgct atcctgcggg gggaacgacc gataacgggg cgctcgtgtt
     5701 tcggccgagc acgagcagac agaactcgtc ttcctcgacg ctcgccaatt tccgaaaacc
     5761 aaactcctcg aatttggcga ggacgtcgtc ggcctgagca cacagaaagc ccgtgaggat
     5821 cagctcgcgc gcatcgcgtg cacggcgagc gatgcgctcg gcgtcggcca gcagggcgcg
     5881 cggctcgaga tttgctacga cgaggtcgta gcccgaagca gccgtcgcag ccgcatcggc
     5941 gaacgagact agctgcgcga ttccattgag ctctgcgttc tcgcgcgcgg cccctagcgc
     6001 aaccgagtcg acgtcgacgc catgagccgt cttcgcacca gagagcgcgg cgagcagcgt
     6061 gaggatcccg ctacccgttc ccacatcgag aacgctggcg cccggcaatg cgagacaaaa
     6121 gcgctcgacg gcttgagcag ccaagcgagt cgtcacgtgc gcgccgtcgc cgaacgcgag
     6181 cgtcggccga atgacgatgc gctccatgcc tggcggaggg ggcgtctcgt cccagagggg
     6241 ctgcaccacc cagcgctccg tcagcgcttg ctgttggagg tgcttggtcc aagcagtgtc
     6301 cca
//
LOCUS       DASTKS010000765         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_568122, whole genome shotgun sequence.
ACCESSION   DASTKS010000765 DASTKS010000000
VERSION     DASTKS010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568122"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..817
                     /locus_tag="VFQ35_15900"
     CDS             185..817
                     /locus_tag="VFQ35_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932187.1"
                     /translation="MRGHVSCLLLAALLVTVSASAEEPAKPESKEATTDSADGVRRDP
                     KGVQGVSPMWEAIGKGDSAVLARDYEAAVAAYREAITKNPQNALAHYRMGEAELLKGD
                     LKEAQAAYEAALRFAGKDKGLTAKALFRLADLNERAKNWDQALARWNAYSEFAKTAES
                     VKTFPATAEERRKRVTEWKQIQSDSAAVKERIEKRAKEAEETAKKAPITK"
     gene            complement(972..>2092)
                     /locus_tag="VFQ35_15905"
     CDS             complement(972..>2092)
                     /locus_tag="VFQ35_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932188.1"
                     /translation="EAMADASVDLTPVDPRIERIARHIVESAPPDHASDRAKLLYHWL
                     VTNVQEGEESDGRRVIIGKNGNLWRGFITLCRAVNIPVDYAVAQSRLTLAASGPFSGA
                     QLYTMPLVRVAVEKGDDWLSLGSKYAPFGYVPADARGMPAFVLSESGPRAVRVPTSGV
                     ADRVTYVGDVRLAADGSADIDLEQTLSGKYATALRGALAEMAAQQVRDLIETRLIGHA
                     LRGAKLEKYDIVNAADPDKPIAIRTRSRVPSFAQVAGGVLLVSPPFAPRVSQLAALPA
                     RQTPLLLADSSEQEIVLTVHPPQGSTLSSPVAPQEISDGEYKVQIRDREQAGAVVLDR
                     RIVLPAGRIQVGAYPEFLRFARKADDALFASVRIRVGR"
BASE COUNT          375 a          654 c          734 g          329 t
ORIGIN      
        1 agttgaccgg cggcggagga gatctcctgg agggtctctt ctacgactct accggcgtcg
       61 ggtaccgcat cagcgggcaa ttcgacggcc cgattcttcg cttccgttct tacgacgcgc
      121 cgtggcgtgg ctcgggcacg atggtccgct gaggctcccg gcgagctgtg ttagcctcgt
      181 gtccatgcgc ggccacgtct cgtgtctcct gctcgctgcc ctgttggtga cggtttcggc
      241 gagcgccgaa gagcccgcca agcccgagtc gaaggaagcc accaccgatt cggcggacgg
      301 cgttcgacga gatccgaaag gggtccaggg cgtgagcccg atgtgggagg cgatcggcaa
      361 gggtgacagt gccgtactcg cgcgcgacta cgaagcggct gtcgccgcgt accgcgaggc
      421 catcaccaag aacccgcaga acgcgctcgc gcactaccgc atgggcgagg cggagctctt
      481 gaaaggcgat ctcaaggaag cgcaggctgc ttacgaggcc gcgctgcgtt ttgcgggtaa
      541 ggacaagggc ctcaccgcga aggcactgtt tcgcctcgct gatctgaacg agcgcgcgaa
      601 gaactgggac caggcgctcg cgcgctggaa cgcgtattcg gagttcgcga agacggcgga
      661 gagcgtgaag acctttcccg ccaccgctga agagcgtcgc aaacgagtga cggagtggaa
      721 gcagatccag tccgactcgg ccgccgtcaa agagcgcatc gaaaagcgcg cgaaagaagc
      781 cgaagagacc gcgaagaaag cgccgatcac caagtaggcg ctcgggcgtc cgttagagcg
      841 acaaacggtt aggaattcgc cgaaggtagc ggaacacggc tccgcacgga gtgaccgcgt
      901 ggggggaggc gcgtgccgcc cccacaaacc aaggagagcc caagcggacg ccggcgcgga
      961 gcgacttcgc ttcagcgccc cacgcgaatg cggacgctcg cgaagagagc gtcgtcggct
     1021 ttgcgcgcaa agcgcaggaa ctccggataa gcgccgactt gaatgcgccc ggcggggagg
     1081 acgatgcgcc gatcgagcac cacggcgccc gcttgctcgc ggtcacggat ctggactttg
     1141 tattcgccgt cgctgatctc ctgaggtgcg acggggctgc tcagcgtgct gccctgcggt
     1201 ggatgcaccg tgagcacgat ttcttgctcg ctgctgtcgg ccaagagcag aggcgtttgc
     1261 cgcgccggca acgcggcgag ctgactcacg cgcggggcga aaggcggcga aacgagcagc
     1321 acgccgcccg cgacctgagc gaacgaaggc actcgcgagc gcgttcggat cgcgatgggc
     1381 ttgtcgggat ccgcggcgtt gacgatatcg tacttctcga gcttggcccc gcgcagggcg
     1441 tgaccaatga gccgcgtctc gatgagatcg cgaacttgtt gcgctgccat ctccgcgagc
     1501 gcaccgcgca gcgcggtcgc gtatttgccg ctcaacgttt gttcgagatc gatgtcggca
     1561 gagccgtccg cggcgagccg cacgtcgccc acgtaggtga cgcgatcggc gacgccgctg
     1621 gttggcacgc gcacggcgcg cggtccggac tccgaaagta cgaacgccgg catgccgcgc
     1681 gcatccgccg gtacgtagcc gaacggcgcg tacttgctgc cgagggagag ccagtcgtcg
     1741 cccttctcga cggccacgcg cacgagcggc atcgtataga gctgagctcc cgaaaaaggg
     1801 ccgctcgcgg cgagcgtcag gcgactctgc gcgacggcgt agtcgaccgg gatgttcacg
     1861 gcgcggcaca gcgtgatgaa gccgcgccac aggttgccgt tcttgccaat gatgacgcgc
     1921 cggccgtcgc tctcttcgcc ttcttggacg ttcgtgacga gccagtgata gagcagcttc
     1981 gctcgatcgg aggcgtggtc gggcggggcg ctctccacga tgtgtcgcgc gatgcgttcg
     2041 atgcgtgggt cgaccggagt caagtcgacg gaggcgtcgg ccatggcttc ga
//
LOCUS       DASTKS010000766         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1461922, whole genome shotgun sequence.
ACCESSION   DASTKS010000766 DASTKS010000000
VERSION     DASTKS010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461922"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..2153)
                     /locus_tag="VFQ35_15910"
     CDS             complement(102..2153)
                     /locus_tag="VFQ35_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932189.1"
                     /translation="MQSELAEGAPPHRPSGVPAAAKWSDSIGAWELVEFDANARRCGT
                     YRSYRVDGSLKFECRYENGLRHGPFRAYHPNGERSREGEFIGDAPHGLVVAYRSDAIG
                     SEPLRACCVPTRAREMRAEYNHGELLLERFFDGDGRLLLSDGNVAPQRPSGVPERALY
                     DESSDRWYTREADGELELVRIFDASGLLTQDDLYLNCRRTRTRRFFGDGTCKAEEHFD
                     DSGQLTGKRTLRYGDGEPSPFANPEIVEECAMFEAGEPVGVTDYFSAAGSVRRSNRGA
                     AFEPADLASPVFGAAELTAAAWSELCDELTRDGRVRLAFCAAARAAARGRSKTPLANW
                     LEENVVELRGDVANELAEALSRGESSDVSRVLSAVLLGAEPSLSLCTLAKCLPGTGQV
                     ALDFVEAAILLAPDNDAAYGMRAMTRLELGLVDQALADAERAAKFSPALTTFVREYAR
                     LLFPTWRFFPRETGIDSPALEGLSEAPRQPLESVRRTITLYATRLQRLRAAAKARLAE
                     GEPDWLPPELTSLMPTGPLELTRATATIVDQTEDGEEASEVIVDETLPIEGASLQTLQ
                     LLARADWAALTWLCWVSGLDHVAMPDTFSPRANFAAAASQAIVRAGRIADVVATGGLR
                     ARMQGAPSFEWEGFDVDGLPASFARIAMTELVQVRAMFLWLCFAENVSPFQGDLQELE
                     G"
     gene            complement(2182..3807)
                     /locus_tag="VFQ35_15915"
     CDS             complement(2182..3807)
                     /locus_tag="VFQ35_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HET9932190.1"
                     /translation="MAHFLHERPLLLLFGVAAIGYLIGRVRIFGFSLGVAAVLFAGLF
                     VGWWLPGIELPELVAQLGLVLFVYTVGLASGPGFFASLRLRGLRDNGLAVGVLTLSFA
                     LSLALGRAFGLSGPALAGLFSGSLTNTPALAAAVDVLKQSGASAEVRSAPVVAYSVCY
                     PLGVLLPLLVVALSERWFKVSYETEPISISYRGLSEEPIINVTVLVERDQTLEAEHLR
                     KIGNFTVNFGRIRRGFKTEIVHDDTLFRAGDLITIVGCQKDVEVATKMLGRNSDEHLE
                     DDRTTVDYRRMFVSRSDVTERPLRELNLLQKFDAVITRVRRGDVDLVPDGRFELMLGD
                     RARVLAPRDRMPEVAKALGDSVRHVAEIDVITFGLGIALGLLLGAVPFPMPGGGSFTF
                     GLAGGPLIAGLVLGRVGRTGSLVWSSPYGANMTLRQLGVVLFLAGVGIKAGGTLGTTL
                     SQGAVLSIALSGALVTLTSVLVAVIVGHRIMRIPLGVMVGTVAGIQTQPAVLAFAVEK
                     TRKDLPNVGYATVYPLVMITKIIMAQLVFRLIG"
BASE COUNT          752 a         1298 c         1321 g          649 t
ORIGIN      
        1 agctcaagct tcgcattccg gctctcggtg gaccggtcgt gaacgagtgg ctcagcgact
       61 tcgaggcgcg gccgagcgcg ctcgccgccg gcgctcgctg atcagccctc gagctcctgc
      121 agatcgccct gaaacggaga cacgttctcc gcgaagcaaa gccagaggaa catcgcgcgc
      181 acctgaacga gctcggtcat ggcgattcga gcaaacgaag cgggaaggcc gtctacatcg
      241 aagccctccc actcgaagct aggggcacct tgcatgcgtg ctcgcaatcc ccccgtggcg
      301 acgacgtcgg cgatgcgtcc cgccctcacg atcgcttggc tcgcggccgc ggcgaagttc
      361 gctctcggcg agaaggtgtc cggcatggca acgtggtcga gtcccgagac ccagcacagc
      421 cacgtgagcg ccgcccaatc ggctcgggcg aggagttgca gcgtttgcag cgaagcgccc
      481 tcgatcggca aggtctcgtc gacgataacc tcggaagcct cctcaccgtc ttccgtttgg
      541 tcgacgatgg tcgcggttgc gcgcgttagc tcgagcggcc cggtcggcat caggctcgtt
      601 agctcgggtg gcaaccaatc gggctctccc tcggcgaggc gcgctttcgc ggcggcgcgc
      661 aaacgctgga gccgcgtcgc gtagagcgta atcgtgcgac ggacggactc gaggggctgc
      721 cgcggcgcct cgctgagccc ttcgagcgca gggctatcga tgccggtctc tctggggaag
      781 aatcgccagg tcgggaacaa gaggcgcgcg tactcacgaa cgaaggtggt gagcgcgggc
      841 gaaaattttg cggctcgctc tgcgtctgcg agcgcctggt cgacgaggcc gagttcgagc
      901 cgggtcatcg ctcgcattcc gtacgcggcg tcgttgtctg gcgcgagcaa gatggccgct
      961 tcgacgaaat cgagcgcgac ttggccggtc ccagggaggc acttggcgag cgtgcacaga
     1021 gacaggctcg gctctgcgcc gaggagcacg gcagagagca ctcgggaaac gtccgaggat
     1081 tcgcctcgac tcagggcttc ggcgagttcg ttggcgacgt cacctcggag ctcgaccacg
     1141 ttctcttcga gccagttcgc gagcggcgtt ttcgagcggc ccctggcagc ggcgcgcgct
     1201 gcggcgcaga aagctagccg cactcttcca tctcgggtga gctcgtcgca gagctcggac
     1261 cacgctgcgg cggtgagctc ggccgcgccg aagacggggc tcgcgagatc cgcgggctcg
     1321 aatgccgcgc cccggttcga gcggcgaacg ctcccggcgg cggagaagta gtcggtcact
     1381 ccgaccggtt ctcccgcctc gaacatggcg cattcttcga cgatttcggg gttggcgaag
     1441 ggactcggtt cgccgtcacc gtagcgcagc gtacgcttgc ccgtgagctg tcccgagtcg
     1501 tcgaagtgct cttccgcttt gcacgtgccg tctccgaaaa agcggcgagt gcgcgtgcgg
     1561 cgacagttca gataaaggtc gtcctgcgtc aacaggccgc ttgcgtcgaa aatgcgaact
     1621 agctcgagct cgccgtccgc ttcgcgcgtg taccagcggt cactcgactc gtcgtagagc
     1681 gcgcgctcgg gaaccccgct tgggcgctgc ggtgcgacgt taccgtccga caagagcagt
     1741 cgcccgtcgc cgtcgaagaa gcgctcgagg agcagctcgc cgtgattgta ctcggcgcgc
     1801 atctcgcggg cccgggttgg cacgcagcac gcgcgtaggg gctccgaccc gatagcgtcc
     1861 gagcggtacg cgacgacgag gccgtgcggc gcgtcgccga tgaactctcc ttcgcgggag
     1921 cgctcgccgt tcgggtggta ggcgcgaaaa gggccgtggc gaaggccatt ctcgtagcgg
     1981 cactcgaact tcagcgagcc gtcgacgcga taactacggt aagtcccgca gcgccgggcg
     2041 ttcgcgtcga actcgaccag ctcccacgct ccgatcgagt ccgaccactt cgccgccgcc
     2101 ggaactccgg aaggccgatg cggcggcgcg ccttcggcga gctcactttg cacgccgcga
     2161 ccatagcacg gcgatcactc ttcatccgat gagccggaac acgagctgcg ccatgatgat
     2221 cttggttatc atcacgagcg ggtacaccgt ggcgtaaccg acgttcggca gatctttgcg
     2281 tgtcttttcg acggcgaacg cgagcaccgc gggctgtgtt tgaattccgg cgacagtgcc
     2341 gaccatcacc cccaacggaa tgcgcatgat gcgatgcccg acgattacgg cgacgagcac
     2401 gctggtcaga gtaacgagcg cgccgctcaa cgcgatgctg agcactgcgc cctggctcaa
     2461 cgtcgtgccc agcgtgccac ctgctttgat gccgacgccg gcgaggaaca agacgacgcc
     2521 gagctgtcgc aaagtcatgt tggccccgta cggagagctc cagacgagcg agccggtccg
     2581 ccccacgcga ccgaggacga gtccagcaat gagcggtcca cccgcgagcc cgaaagtgaa
     2641 gctgccgcct cccggcatcg gaaacggaac cgcaccgagc aacagcccga gcgcaattcc
     2701 gagcccgaag gtgatgacat cgatttcggc gacgtggcgt accgagtcac cgagcgcctt
     2761 cgcgacttcg ggcatgcggt cgcgcggagc gagcacgcgc gctcggtcgc cgagcatgag
     2821 ctcgaagcgc ccgtcgggta cgaggtcgac gtcaccgcgc cggacgcgag tgatcacggc
     2881 gtcgaacttt tgcaagaggt tcagctctcg cagcggccgc tcggtcacgt cggagcggga
     2941 tacgaacatg cgccggtagt cgaccgtcgt gcgatcgtcc tcgaggtgtt cgtcgctgtt
     3001 gcggccgagc atcttggtcg cgacttccac gtctttttgg cacccgacga tcgttatcaa
     3061 gtcaccggcg cgaaacagag tgtcgtcgtg aacgatctcg gtcttgaagc cgcgccggat
     3121 gcggccgaag ttcacggtga agttgccgat cttgcgcagg tgctccgctt ccagcgtctg
     3181 gtcgcgctcg acgagcaccg tgacgttgat gatgggctct tcgctcaggc cacgatacga
     3241 aatcgaaatg ggttccgttt cgtacgagac cttgaaccac cgctcgctca gcgcgacaac
     3301 caagagcggc agcagcaccc cgagcgggta acacacggag taagccacga ccggcgcgga
     3361 gcgaacctct gcgctcgcgc cggattgctt caatacgtcc accgcggcgg ccagcgccgg
     3421 agtgttcgtc aacgagcccg agaacaaacc cgcgagggca gggccgctga gcccgaaggc
     3481 gcggccgagc gcgaggctta gcgcgaacga gagtgtcaac acaccgactg cgaggccgtt
     3541 gtcgcgaagt ccgcgcaagc gcagcgacgc gaagaacccg ggtccactcg cgagtccgac
     3601 ggtgtaaacg aacagcacca ggccgagctg cgcgacgagt tcgggcagct cgatgcctgg
     3661 tagccaccat cccacgaaga ggcccgcgaa caacaccgcg gcgactccta gggaaaagcc
     3721 gaagattcgg acacgaccaa tcaagtaacc gatcgcggcg acgccgaaga gcagcagcaa
     3781 aggccgctcg tgaaggaaat gagccatgaa atctttctga gcgaaggtct aaggcgacaa
     3841 agccggactc aatccaagtg actaaactgt ggacgttcca tgttctcgcg tgggctcagt
     3901 cttttgaacc cgtgtgaaag aggggcgacg tttgccgccc tcgctactta cacccaagct
     3961 cgcttgggtg gatggaacaa aacgatacac ctggcgacag attgccccaa aggaacgcgg
//
LOCUS       DASTKS010000767         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_568657, whole genome shotgun sequence.
ACCESSION   DASTKS010000767 DASTKS010000000
VERSION     DASTKS010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568657"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3105
                     /locus_tag="VFQ35_15920"
     CDS             <1..3105
                     /locus_tag="VFQ35_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9932191.1"
                     /translation="RSNEDAESQRSPLVLAGAATACAAPPPAPRASYADQAAVVQNLA
                     SNAQYDVLTQHNDISRTGAAVHESILTPAAVKGGFGLLASVKVSGKIYAQPLYVEQSA
                     VSCGGGAVTNANIAYVATLANVVHAIDVDTHTICWSSSPLGCGQAADGLLGFNPNSSE
                     GVGAVSVGIVSTPVIDLARSVLFAVTREWSGSPAATHFFVNALDTRTGALVGRAEVTG
                     AGSAADCGGHPFEPRAHNNRPGLLLVQNQLFLGFGSTIGEDAGTPYHGFVLGFDVSDP
                     THPTALAHAFCSTPNGTGGGIWQSGAGLASDGTSIYLLTGNGAYTLSPGGGPPTNVPE
                     LPSPNNYPDSFVKIPLAYFTNGAASPPTGYTDTRYAWQMPAIVASPNQPTPVPAWGLK
                     AGSTNHTLFWGREYSDADLGSGGAVLFGSRVIGGGKDGILYLLNRADMAHVQSMQAFV
                     DADNALGTDTSYFANYSYMTQWYSGPNIHGAPIVWDVSARGGSAGAFVYGWSEKDSLK
                     RFEIRNGSFTSTASATPWNPTPTPHGEVMSAFRSMPGAMLSLSSNGATGGVVWATLEE
                     PYPDAGIVGMTPRCGSGFGANEPCAGCMVGPSKTTFVEHCDATQGYVAGRLYAFAADD
                     NGRGLLPLLWGDKRVSVGTAPNNRIARYSKFTAPTVAHGKVLVPTGNDELLIYGLAAQ
                     APRARRADDLMAAWNYGGYASFAMYASSKTDFAPHIVWAAQEGGFDGSRWMASDYDGD
                     GRSDIGAAWPNGYSGLPLLNSLAVRRTLGNATPPFESPAQWAGSLQNGNWVAGTDGTW
                     VESSQWVSGDFDGDGRGDVGVVWNDGSNSASFSVYRAASNGARFERSFPWSIHDGGWL
                     DPPYITWLAGDFNGDGLADLVAIWDDAGYNTLTVRLSNRSAFAPQWHWLVKSGDYATN
                     TRWLVGDFDGDGLADVASAARSGLGAAFTIFRSNGTSFVRGTTSAPDGGWSDTIKWIA
                     GDFDADGKSDIAAVWNNGGRNAIAMRRSFGTGFYGGVSWMRAGLDYGGWMDSTNWLAG
                     KFQR"
     gene            complement(3156..3515)
                     /locus_tag="VFQ35_15925"
     CDS             complement(3156..3515)
                     /locus_tag="VFQ35_15925"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9932192.1"
                     /translation="MHENVGNTDRWLRVAVGGALVLGAVSSLGARRAFGPGLLLATGA
                     LLIETALTRVCPMSAALGVDSRDWEPFARLGASSEKGDADGRNDADDSGRRELGRVRE
                     APGGTEALRSAQPEVAT"
     gene            complement(3607..>4035)
                     /locus_tag="VFQ35_15930"
     CDS             complement(3607..>4035)
                     /locus_tag="VFQ35_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932193.1"
                     /translation="TVAVSRAATRGITEELGATDATNANGRAGSSRIDALAKQLSNAF
                     AVGFEDAVDRSMVRRNQGHERDAPVLASLGRAATTSFDILSLGGAIAVLLFLGLVGAL
                     VALFVRARRYRRESELRDNVLSNLLRLHPELTSREVHAGS"
BASE COUNT          691 a         1350 c         1334 g          660 t
ORIGIN      
        1 cgctcgaacg aagacgccga aagtcagcga agccctctcg tcctcgccgg ggcggcgacg
       61 gcctgcgccg cgcctccgcc cgcgccgcgc gcttcgtacg cagaccaagc cgcggtagtg
      121 cagaacctcg cgagcaacgc ccagtacgac gtgctcacgc agcacaacga catttcgcgc
      181 acaggagccg ccgtccacga aagtattctc acgcccgcgg cagtaaaagg cgggttcggt
      241 ttgctcgcgt cggtaaaagt cagtgggaag atctacgcgc agccgctcta cgtcgagcaa
      301 tccgccgtct cgtgtggcgg cggcgcggta acgaatgcga acatagcgta cgtcgcgacg
      361 ttggcgaacg tggtgcacgc gatcgacgtc gacacgcaca cgatctgctg gagcagctca
      421 ccgctcgggt gtggccaagc cgcggatggg ttgctcgggt tcaacccaaa ttcgagcgag
      481 ggtgtgggcg cggtgtcggt cggcatcgtg agcacgcccg tgatcgatct cgcgcggagc
      541 gtgctgttcg cggtcacgcg cgaatggagc ggctcaccgg cagcgacgca cttcttcgtg
      601 aacgcgctcg acacgcgcac gggcgcgctc gtcgggcgcg cagaggtgac cggagcggga
      661 tcagcggcag attgcggcgg gcaccccttc gaaccgcgcg cgcacaacaa ccggcccggc
      721 ttgctgctgg tgcaaaacca gctctttctc ggcttcggca gcacgatcgg cgaggacgcg
      781 ggcacgccct atcatggctt cgtactcggc ttcgatgtga gcgacccgac gcacccgacc
      841 gcgctcgcgc acgccttctg ctcgacgccg aacggcaccg gtggcggcat atggcaatcg
      901 ggcgcggggc tcgcgtcgga cggcacgtcc atttacctgc tcacgggcaa cggcgcgtac
      961 acgctgtcgc ccggcggtgg gccgccgacg aacgttccgg agctcccgtc gcccaacaac
     1021 tacccagata gcttcgtcaa gattccactc gcgtacttca cgaacggcgc ggcctcacca
     1081 ccgactgggt acaccgacac gcgttacgca tggcaaatgc cagcgatcgt tgcgtcgccg
     1141 aatcagccga ccccggttcc ggcgtgggga ctcaaagccg gcagcacgaa tcacaccctc
     1201 ttttggggcc gcgagtattc cgatgccgac ctcggctcgg gtggtgcggt gctcttcggc
     1261 agccgcgtca tcggtggcgg aaaggacggc atcctgtatc tgctgaaccg tgccgacatg
     1321 gcccacgtgc agtcgatgca agcattcgtc gacgcggaca acgcactcgg caccgacacg
     1381 agctacttcg cgaactactc gtacatgacg cagtggtact cgggcccgaa catccacggc
     1441 gctccgatcg tgtgggacgt gagcgcgcgc ggcggctccg cgggtgcatt cgtgtacggt
     1501 tggagtgaga aggactcgct caagcgcttc gaaattcgaa acggctcgtt cacgtcgacg
     1561 gccagcgcga cgccttggaa cccgactccg acgccgcacg gcgaggtcat gagcgcgttc
     1621 cgctccatgc cgggggcgat gctgtcgttg tcttccaacg gcgcgacggg cggggtcgtc
     1681 tgggcgaccc tcgaggagcc gtacccggac gcgggcatcg tcggcatgac gccgcgctgt
     1741 ggttcggggt tcggggcgaa cgagccgtgc gcggggtgca tggtggggcc gagcaagacc
     1801 actttcgtcg agcactgcga cgcaactcaa gggtacgtcg cggggcggct ctatgccttc
     1861 gccgcggacg acaacggtcg cgggctcttg ccgttgcttt ggggcgacaa gcgtgtgtcc
     1921 gtcggcacgg caccgaacaa ccgaatcgcg cggtattcga agttcacggc accgacggtc
     1981 gcgcacggca aagtgctcgt ccccactggc aacgatgagc tcctcatata cgggctcgcc
     2041 gcccaagcgc cgcgcgcacg ccgcgcggat gacctcatgg cggcgtggaa ctatggcggg
     2101 tacgcgtcgt tcgccatgta tgcctcgtcg aagacagact tcgcacccca catcgtgtgg
     2161 gcagcgcaag agggcggctt cgatggctct cgctggatgg cgagcgacta cgacggcgac
     2221 ggccgcagtg acatcggcgc ggcgtggccg aacggctact ccgggctccc gctgttgaat
     2281 agcctcgccg tgcgccgcac cctggggaac gcgaccccgc cgttcgagtc gccggctcag
     2341 tgggctggct cgctgcaaaa cgggaactgg gtcgccggca ccgacgggac gtgggtcgaa
     2401 agctcgcagt gggtgagcgg tgatttcgac ggtgacggcc gtggcgacgt cggcgtcgtg
     2461 tggaacgacg gcagcaactc ggcaagcttc tccgtgtacc gagctgccag caacggcgcc
     2521 cgtttcgagc gcagctttcc ctggtcgata cacgacggcg gctggctcga cccgccgtac
     2581 atcacttggc tcgcgggcga cttcaacggc gacggtctcg ctgatctcgt cgcgatctgg
     2641 gacgacgccg gatacaacac gctcacggtg cggctctcga atcgttccgc cttcgccccg
     2701 cagtggcact ggctcgtgaa gagcggcgac tacgcgacga acacgcgctg gctcgtcggt
     2761 gacttcgatg gcgatggcct agccgatgtc gcgtcagcgg ctcgcagtgg gctcggcgcc
     2821 gcgttcacca tctttcgctc gaacgggacg agcttcgtcc gagggacgac gagcgcgcca
     2881 gacggcggct ggtcggacac catcaagtgg atcgccggtg acttcgacgc cgacggcaag
     2941 agcgacattg ccgcggtatg gaacaacggc gggcgcaacg cgatcgcgat gcgccgctcg
     3001 ttcggcaccg gattctatgg cggcgtgtcc tggatgcgcg ccgggctcga ttacggcggc
     3061 tggatggact cgaccaactg gcttgcgggc aagttccagc gttaatcgac gcacgcgagc
     3121 ggacggcgcg tcaaggcgac ggcaaagctc gacgcctagg tagcgacttc gggttgagcg
     3181 cttctcagcg cctcggttcc gcccggagct tctctcacgc gaccaagctc tctgcgtccg
     3241 gagtcgtctg cgtcgttgcg cccgtcggcg tcacccttct ccgaacttgc gccgagccgc
     3301 gcaaagggct cccagtcgcg tgagtcgaca ccgagcgccg cactcatggg acacacgcgt
     3361 gtgagcgcgg tctcgattaa aagcgcaccc gtcgcgagca gtagcccagg cccgaacgcc
     3421 cggcgagctc cgagggaact cactgcaccc aaaacgagcg ctccgcctac ggcgacgcgc
     3481 agccagcgat ccgtattgcc aacgttctca tgcatgacat cgactcctcg ggatccgcga
     3541 tcccatcttc gaatgaactc cgagttcaga tgaaccctcg cgctatcgag gtcgtgctcg
     3601 ctcgaatcac gaccctgcat ggacctctcg cgaagtgagc tccggatgca gacgcagcaa
     3661 gttcgagagc acgttgtccc gcaactcgct ctcgcgtcgg tatcgacgag ccctgacgaa
     3721 gagcgcaacc agggcgccga cgaggcccaa aaagagcagc accgcgatgg cgcctcccag
     3781 cgaaagtata tcgaagctcg tcgtcgcagc gcgcccgagc gacgcgagca cgggcgcgtc
     3841 gcgttcatga ccttggttgc gccgcaccat gctcctatcc actgcatctt cgaagccaac
     3901 cgcgaaggcg ttcgagagct gtttcgcgag cgcgtcgatc cggctgctgc cggccctgcc
     3961 gttggcgttc gtcgcgtccg tggccccgag ctcttcggtg atgccgcgcg tcgcggcgcg
     4021 gctgaccgcg accgt
//
LOCUS       DASTKS010000768         7436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1238709, whole genome shotgun sequence.
ACCESSION   DASTKS010000768 DASTKS010000000
VERSION     DASTKS010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238709"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1368
                     /locus_tag="VFQ35_15935"
     CDS             292..1368
                     /locus_tag="VFQ35_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932194.1"
                     /translation="MDALFAEATASLRSDAWWRKRIATLSKGLGAELDDGLVVRARER
                     ALWPLAEYVVARAPATLRARAGRRAWPFERALDALGKRREQFARATLRAGFSRGHLLE
                     IVVNLPGATGNEDDLGAAESLVWNVLGERLANDWVGAVKVAPAPRGGPLRVLATAKES
                     GFALSELDAAVAAAIAGVYDGLPKEPHWARNDSDEWTLFELEAEPADDWADEDDLVMA
                     TTSVPELLKSRLTKQPFSSLRFSRAGELFLHLKYESDGEPEERLAQRQRLEDALDRSL
                     IERKRGRVVGAGIGLRYSYVLLAVVDAERTATAIAELARPHVSSKRAWLIPLDTDLAH
                     EWCGVWPDSPAPYGLVPEARNALE"
     gene            complement(1318..2388)
                     /locus_tag="VFQ35_15940"
     CDS             complement(1318..2388)
                     /locus_tag="VFQ35_15940"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9932195.1"
                     /translation="MTAAPFPALPSLERLVESATGTQPPLEYEEIVGGGSVRRFFRVR
                     TRDGGSVVAMYAPSHTHEVGSDPASRRPWPFLEVRDLLEQHGVRVPSLVATACHDGLI
                     LVEDLGETLAQHLLRCPENRETLYRIAVRELGRAQRALSPVPPTSIVRGRAFDERLLR
                     WEIDHFKEWALEARGIHLEPNDAAIFDRAAAHLAAMISDWPRTFVHRDYQSRNLMVVS
                     GPGNDLGLAWIDFQDAMLGPRVYDMVALLSDSYQTFTREFVEARLDEFTQHLGLDRNE
                     RARIGREFRVVTVQRKLKDAGRFVFFDRMNNNPSFLRFVEPTIEKARAALRELTDDPI
                     LAELGRLLERVPSLGHEPVRRG"
     gene            2572..3219
                     /locus_tag="VFQ35_15945"
     CDS             2572..3219
                     /locus_tag="VFQ35_15945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET9932196.1"
                     /translation="MSEATQLRARISRVLSAFGGERPLANDAALEQLLLWTTRVLEWN
                     QRIDLTAARSHDELVDLLVADAAAIAMVGAGRLSGAWVDVGSGAGGPGLGLALLVPES
                     SLTLVEPKAKRVAFLRSVLGELAVSRVRVQRARSDELPSGTFDGALSRATFSPGEWLK
                     EGARLARTHVWVLLAQAEAPPATEFRPVLDHRYVWPLTGAERRLVLYVRADDVAG"
     gene            3544..4080
                     /locus_tag="VFQ35_15950"
     CDS             3544..4080
                     /locus_tag="VFQ35_15950"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9932197.1"
                     /translation="MYRRTAVIAVAACLTSIALAAHAMSGGGGHIDVQGSASPALGSF
                     SFESSDVKVSESDGKVIFVTNLGGMKMGLRKSHCHEAFEVPKHPNAKLTVDKSKLKIP
                     AAGATEKGSVQGELTLHGVTKPVTVKYTATGEGNGYKVGGSFTFKYEDFKVSKICKVG
                     VCVDPAVNVTVKDVHVNG"
     gene            4427..5947
                     /locus_tag="VFQ35_15955"
     CDS             4427..5947
                     /locus_tag="VFQ35_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932198.1"
                     /translation="MHYRSRRGPSSFAASRLALRRFLAALFAFVCIALSHRAEAYPWM
                     IRHGYTNCSGCHADPSGGELLTVYGHAISYEALSTKWGGSSSEARAYEQRVARAISRA
                     AHAKAANKKDEDADDEADADEPEGKAKGDGSDKAKGEGDADEGEAEEGAAEEEGASSE
                     ASSTEAPSSSGGDDNPFEGMSGITGPLFGLMKPSDTLLLGGAVRVATIHKFDAGTTRV
                     FPMQLDLYGQLRLGSLRFGGSVGAAKVPAGSLLARPAQVTSNQGDGYNVISRTHWVGF
                     DFGNGMHTIRAGRLNLPFGLRMSEHTMWVRDKTQTNRESQQQHGVALFMGFDKVRFEI
                     MGIAGNLQANPDKFRERGYSGYVEFVVGDRATLGVNSLYTESKADPIYNEDLTTDRGA
                     HGLFARAALGKALVVMGEADVLTRSRRELGYVGFLQLDAEVISGLHLLASGEVLDAGY
                     PKNGGPSRVARIAGQGKTGLGGWFGAQWFFLPHFDFRVDAIVRQDTQLLGQLHIYL"
     gene            5956..6462
                     /locus_tag="VFQ35_15960"
     CDS             5956..6462
                     /locus_tag="VFQ35_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932199.1"
                     /translation="MNDALSPSVRARALRAFFAAMALGCVAMVADPAAAKPEFPAELA
                     KGANMGCVPQCTVCHTVNPGVAGTGLLKPFPKALVGYNLLGDGAEKAFEAMKAENKPE
                     YQSMIQAIEDDHDPNYPLQLACGPAYGCGAHVAKKPPRNYWGAGWVLGVLSAYALARR
                     LKSRAAQR"
     gene            6645..6935
                     /locus_tag="VFQ35_15965"
     CDS             6645..6935
                     /locus_tag="VFQ35_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932200.1"
                     /translation="MLDEVAAEVTLRGESVVRAAAERQIFQRVLAALCKRRQMMKFEP
                     VRLSAALPRAVDIRAAPFIALEDRTPDRGRHISPALPALARYFLFPGGRVRC"
BASE COUNT         1239 a         2386 c         2472 g         1339 t
ORIGIN      
        1 tcgtactacc cgtgttcatc gcgcgacgcg cgcggagggc ctggcaggcg tttcgccatg
       61 tcccgagtgc ggcccccgcc ggggctcgaa ctcgtcggcg tgaggcttcg ggaccaagag
      121 ctttgtcgcg cgtttgcgcc gaggcgcctc gctcgccgcg agagtgaggc gccgccgcgc
      181 acgggcggcg ttgtgaaatc gcgggcgctg gcctaaattc gtgaggtgcg ccttcgtttc
      241 ggcccccgta caccaagcga acagaagaag ctcgaagcgt tcacgagtcg agtggacgcg
      301 ctattcgccg aagcaaccgc gtcgcttcgg tccgacgcct ggtggcggaa gcgcatcgct
      361 accctctcga aagggctcgg cgccgagctc gacgatggcc tcgtcgtgcg cgcccgcgag
      421 cgcgcgctgt ggcccctcgc cgaatacgtc gtcgctcgcg ccccggcaac gcttcgagca
      481 cgcgctggcc ggcgcgcctg gccgttcgaa cgtgcactcg acgcgctcgg aaagcgccga
      541 gagcaattcg ctcgcgctac gttgagggcg gggttcagcc gcggtcacct cctcgagatc
      601 gtcgtgaacc ttcccggcgc gacgggcaac gaggacgacc tcggcgccgc cgaaagtctg
      661 gtgtggaacg tcctgggaga aaggctcgcg aacgattggg tgggcgcggt gaaggtcgct
      721 cccgcgccgc gcggagggcc gctgcgcgtg ctcgccaccg cgaaggaaag cggctttgcg
      781 ctgagcgagc tcgacgcagc cgtagccgcc gcgatcgcgg gcgtttacga cggcttaccg
      841 aaagagccgc actgggcgcg caacgactcg gacgagtgga cgctgttcga gctcgaagcg
      901 gagcccgccg acgactgggc cgacgaagac gacctcgtca tggcaacgac ctccgtgccc
      961 gagctcttga aatcgaggct gaccaagcag cctttttcat cgttgcgctt ctctcgcgcc
     1021 ggtgagctct tcttgcacct gaaatacgag agcgacggcg agcccgaaga gcggctcgct
     1081 caacgccagc ggctcgagga cgcgctcgac cgctccctca tcgagcggaa gcgcgggcgc
     1141 gtggtcggcg cgggcatcgg cttacgctac tcgtacgtat tgctcgccgt cgtcgacgcg
     1201 gagcgcacgg ccaccgccat cgccgaactc gctcgtcccc acgtttcctc gaagcgagcc
     1261 tggctcatcc cgctcgacac cgacctcgcg cacgaatggt gcggcgtatg gcccgactca
     1321 cccgcgccgt acgggctcgt gcccgaggct cggaacgcgc tcgagtaggc gaccgagctc
     1381 cgccaagatc gggtcgtcgg tgagctcgcg cagcgcggcg cgcgccttct cgatcgtcgg
     1441 ctccacgaag cgcaggaacg acggattgtt gttcatgcga tcgaagaaga cgaagcgtcc
     1501 ggcgtccttc agcttgcgct gcacggtgac gacgcgaaac tcgcggccga tgcgtgctcg
     1561 ctcgtttcgg tcgagcccga ggtgctgcgt gaactcgtcg agccgcgcct ccacgaactc
     1621 ccgcgtgaag gtctggtagc tgtcgctgag cagcgccacc atgtcgtaaa cacgcggccc
     1681 cagcatggca tcctggaaat caatccaagc gaggccgagg tcgtttccgg ggccactcac
     1741 caccatgagg tttcggctct ggtagtcgcg gtgcacgaac gtgcgcggcc agtccgagat
     1801 catcgcggcg aggtgcgcgg cggcgcgatc gaaaattgcg gcgtcgttcg gctcgagatg
     1861 aattccgcgc gcttcgagcg cccactcttt gaagtggtcg atctcccagc gcagcaagcg
     1921 ctcgtcgaag gcgcgaccgc gcacgatcga ggtgggtggt acgggcgaca gcgcgcgttg
     1981 ggcccgcccg agctcgcgaa ccgcgatgcg gtagagggtc tctcggttct ccgggcagcg
     2041 cagcagatgc tgggccaacg tctcgccgag atcctcgacc aagatgagcc cgtcgtgaca
     2101 agccgtcgca acgagagacg gcacgcgtac gccgtgctgc tccagcaggt cgcgcacctc
     2161 gagaaagggc cacggccgac ggctcgccgg atcactgccg acctcgtgcg tgtgcgacgg
     2221 agcgtacatc gcgacgacgc tgcctccatc tcgcgtgcgc actcggaaga agcgccgcac
     2281 ggagcctccc ccaacgatct cttcgtactc gagcggcggc tgggtgccgg tggccgattc
     2341 gaccagccgc tcgagcgaag gcagtgcagg gaagggggcc gcagtcacag ctcctacttt
     2401 tagcctcgtt ccagtccctt tgctaccggg gcacactttt cgggtgtgcc ccgagttcct
     2461 ctcggtgagt taacacgctt atttttcagg gtttcgctgc ggcggcgggc gtgggctgga
     2521 cgaagcgcgc catagtgaca agtttgagcc cccgaccaat cgctctcgtc catgagtgaa
     2581 gcgacccagc tccgagcccg catctcgcgc gtgctctctg ccttcggcgg cgaacgcccg
     2641 ctcgccaatg acgccgctct ggagcagctg ctgctttgga ccacgcgcgt cctcgaatgg
     2701 aaccagcgca tcgatctcac cgccgcgcgc tcgcacgatg agctcgtcga cctgctcgtc
     2761 gctgatgccg ccgcgatagc gatggtaggc gctggtcggc tgagcggcgc ttgggtcgac
     2821 gttggaagcg gtgcaggcgg accaggcctc gggctcgcgc tgttggttcc ggagtcgtcg
     2881 ctgacgctgg tcgagccgaa ggccaagcgt gtggcgtttc tccgcagcgt gctcggggag
     2941 ctcgcggtgt cgcgggtgcg ggttcaacgc gcgcgatcgg acgaactccc gagcggcacc
     3001 ttcgacggcg cgttgtctcg cgccacgttc tcacctggcg aatggctgaa ggaaggtgcg
     3061 cggctcgcac ggacacacgt gtgggttctc ttggcgcagg ctgaagcacc gcctgcaacc
     3121 gagtttcgtc cggtgctcga ccacaggtac gtttggcccc tgaccggagc ggagcggcgc
     3181 ctggtcttgt acgttcgcgc ggacgatgtg gcggggtgag atctggtcgc ttgcgagcgg
     3241 gcattcggtg ggcaacggtg cgctgaaccg ctctagcttc tctgaaaaat atcaattccg
     3301 cgggattttc caggtttcgg gaagccttcg gggtggatga acgtagaact ttgacggatt
     3361 gcgcgtcgtt gatggactgg ctttttttat gaatgtcgcg cgttcgtcgc ggcacttgat
     3421 taggacccca tcatctgcaa gcaaggcgtc atcaggtgcc gaaccccgat tcaatcgctg
     3481 actgaatagg cttccggatc gcctcgtcga tttctcgccg tatattacga agaaggagtg
     3541 cccatgtatc gccgtacagc cgtcattgca gtcgccgctt gtttgaccag catcgcgctc
     3601 gccgctcacg cgatgagcgg gggcggggga cacatcgatg ttcagggctc ggcgagtccc
     3661 gcgcttggct cgttcagttt cgagtcgagt gacgtcaagg tctccgagtc ggacggcaag
     3721 gtcattttcg tcacgaacct cggcggcatg aagatggggc tgcgcaagag ccactgccac
     3781 gaagccttcg aggtaccgaa gcacccgaac gccaagctca ccgtcgacaa gagcaagctg
     3841 aagatcccgg ctgccggcgc caccgagaag ggcagtgtcc aaggtgagct caccctccac
     3901 ggtgtgacca agccggttac cgtgaagtac accgctaccg gggaaggtaa cggctacaag
     3961 gtgggcggtt ccttcacgtt caagtacgag gatttcaagg tttccaagat ctgcaaggtc
     4021 ggcgtgtgcg tcgaccccgc cgtcaacgtg acggtcaaag acgtccacgt gaacggctga
     4081 gagctcctgc agcgcgctcc gcgcggcgcc ggcgtaagtc gccgaggtgt ggagcgcgcg
     4141 tcgacgtcgc gatgagatac gcctcggccc gcgttggttc gcgccgaggc gtgccgccgc
     4201 gagtcgtgct cgaagcgttg cctgtgccga cgagtgcgca cagaagtgag aacgcttcgg
     4261 cgagtgcgca cgaacgcgag agcgcttcca cgagacgctc cgagttgaga ctcgtccaac
     4321 tccggaccga gaagctcggc gccatcggcg agtctcgtag agcagatgcc atcaaatgcg
     4381 ttttccggaa ttgacacggc tttcggttgc gatgaagagt cgacccatgc actaccgcag
     4441 ccgacgcggc ccctcgtcgt tcgccgcgtc tcgactggct ttgcgccgct tcttggccgc
     4501 gctcttcgcc ttcgtttgca tcgcattgag ccaccgcgcc gaggcgtacc cttggatgat
     4561 tcggcacggc tacacgaact gcagcggctg ccatgcggat ccatcgggcg gcgagctcct
     4621 caccgtgtac gggcacgcca tttcgtacga ggcgttgagc accaagtggg gcggctcttc
     4681 gtcggaggcc cgtgcgtacg agcaacgcgt cgcgcgcgcg atctcacgcg cggcgcacgc
     4741 caaggccgcg aacaagaagg acgaggacgc ggatgacgag gccgacgccg acgagccgga
     4801 gggcaaggcg aaaggcgacg gctcggacaa ggccaaaggt gagggcgacg cggacgaggg
     4861 tgaggctgag gaaggtgccg ccgaagaaga gggcgcttcc agcgaagcga gctcgaccga
     4921 agcgcctagc agtagcggtg gcgacgacaa ccctttcgag ggcatgagcg gcatcacggg
     4981 gcctctgttc ggcctcatga agccgagcga cacgttgctg ctcggtggcg cagtgcgggt
     5041 cgctacgatc cacaagttcg acgccggcac gacgcgcgtg ttcccgatgc agctcgactt
     5101 gtacgggcaa ctcaggctcg gcagcttgcg cttcggcgga agcgtgggtg cggccaaggt
     5161 ccccgccggt tcactgctcg cgcgtccggc gcaggtcacg tcgaaccaag gcgacggcta
     5221 caacgtcatc tcgcgcacgc actgggttgg cttcgatttc ggaaacggca tgcacacgat
     5281 ccgtgctggc cgcttgaatt tgccgttcgg gctccgcatg tccgagcaca ccatgtgggt
     5341 gcgcgacaag acgcagacca atcgcgagtc tcagcagcag cacggcgtcg cgctcttcat
     5401 gggcttcgac aaggtgcgct tcgagatcat ggggatcgcc ggtaacctcc aagcgaaccc
     5461 agacaagttc cgtgagcgcg gttactccgg ctacgtggag ttcgtcgtcg gcgatcgcgc
     5521 gacgctcggg gtcaacagct tgtacaccga gtcgaaagcc gatccgattt acaacgagga
     5581 cttgacgacg gaccgtggcg cgcacggcct cttcgctcgg gctgcgctcg gcaaagcgtt
     5641 ggtggtcatg ggcgaggccg acgtgctcac tcgttcacgc cgcgagctcg gctacgtcgg
     5701 cttcttgcag ctcgatgccg aggtgatcag cggcttgcac ctgctcgcct cgggcgaggt
     5761 gctagacgcc ggctacccga agaacggcgg accaagtcgc gtcgcgcgca tcgccggtca
     5821 aggcaagacc ggcctcggcg gttggttcgg cgctcaatgg ttcttcttgc cgcacttcga
     5881 ttttcgggtc gacgccatcg ttcgccaaga cacgcagctt ctcggccagc tgcatatcta
     5941 cctgtgagag acgagatgaa cgacgcgctt tccccctccg ttcgtgcgcg cgcacttcgc
     6001 gcattcttcg cggccatggc tcttggctgc gtcgcgatgg tcgcagaccc tgccgcggcc
     6061 aagccagagt tcccggccga gctcgccaag ggcgccaaca tgggctgcgt gccgcaatgc
     6121 accgtgtgcc acacggtaaa ccctggcgtc gccggcaccg gcttgctgaa gccgtttccc
     6181 aaagccctgg tcggttacaa ccttttgggt gatggggccg agaaagcctt cgaagcgatg
     6241 aaggcggaga acaagcccga gtaccagtcc atgatccagg cgatcgagga cgaccacgac
     6301 ccgaactatc cgctgcagct cgcctgcggt cccgcctacg gttgcggggc gcacgtcgcc
     6361 aaaaagccgc cgcgcaacta ttggggcgcc ggttgggtgt tgggagtgct ttcggcgtac
     6421 gctctcgcgc gtcgcctgaa atcgcgcgcc gcccaacgct aagggttcaa tgccgttcat
     6481 ttagtgagct caacggcggg gcgtttgcgg tcgtagctgc tcatcttgat tttgacagcc
     6541 cctaaaacgg tcgcctagcg gcaactactc tgacaccgag tggttcctcg acccattcgt
     6601 cctctttagt gtctgcacgg ttcgtgtcgc gctgagtcgc gacgttgctg gatgaagtcg
     6661 ctgccgaagt cacgctccgc ggcgagagcg ttgtgcgcgc ggcagcggag cgccagattt
     6721 tccagcgtgt gctcgccgcc ctttgcaaac gccgtcaaat gatgaagttc gagccagtgc
     6781 gtctctcggc agcgctgccc cgcgccgtcg acatacgcgc agcgcccttc atcgcgctcg
     6841 aagaccgcac gccggaccgc ggcaggcaca tatctcccgc gctgcccgcg ctggcgcggt
     6901 acttcttgtt ccctggtggt cgcgtccggt gctgagcttg ggcttcgcgt gggacacgtg
     6961 agcgccgttg cggctgcccc agtagcgcgc gagctcggtg ttgcttcagg ccgcgcactc
     7021 gccgttgcgg ccgcggctgc gggaggaagc tcagctgcgg ttgcgtccca agctgcgttt
     7081 gccggccgat ttctcgcagc gcgcttcttt cttgcaagct ccgtcacgag agcgcgcatc
     7141 gctcggagtt ggagttcctc cagggagacg cgcttggcgg agtgagagag cagtgcttgg
     7201 gcttcttcca ccagcttcac gtactcttcg gtagcagtga actgaacgcg acagcgttga
     7261 ggttcgagcc gcgccggcac aggtagtgca cccaacgctt gcgcggtctc cggcagcgtg
     7321 ttcacgggcg ctgggcatgc aggtggcgga tgcagcgctt gcgtagagtt aggcagcgcg
     7381 ttcatattcg gcgcgctcac tggcggcgca ggtactcgca tcgcttcagg cccgtc
//
LOCUS       DASTKS010000769         4909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1014442, whole genome shotgun sequence.
ACCESSION   DASTKS010000769 DASTKS010000000
VERSION     DASTKS010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014442"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFQ35_15970"
     CDS             <1..573
                     /locus_tag="VFQ35_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932201.1"
                     /translation="AAETSAAAILTKGLEQKLLRQEAWINELEARAATADERADAAES
                     ELDEVRERLGELEQRHEREATALTQERDALKAELERLRKRVNDQDDLIALKDTELAAL
                     ADDGEATRELARLEAQLKERGERVLSLEREVFEAERIGRELLRKLDTSAGAASQQLAE
                     QLARAEAELLTLRWSLDLAKRPAHNGVPQS"
     gene            657..1751
                     /gene="dnaJ"
                     /locus_tag="VFQ35_15975"
     CDS             657..1751
                     /gene="dnaJ"
                     /locus_tag="VFQ35_15975"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9932202.1"
                     /translation="MSEKRDYYEVLGLSQGCSSDEIRKAYKQCALKYHPDRNPGDKSA
                     EAKFKEATEAYTVLSDDDKRRTYDRFGHAGLDGAGVDFSGAGVNDILNHFQDMFMDFF
                     GGMGGPQQGRRRRAERGQDVGVAVELTLAESMLGGKREVTVRGPAACETCHGKGTKSG
                     SAPAKCQACRGTGQVTAQRGFIMFASPCARCGGSGTFIQDPCETCNGAGSVERQRRVL
                     VTFPAGIDSGQRLRVPGQGMPGPEGTPAGDLYVDVELTAHPQFERHGDSLGTRRRVSF
                     STAALGGEVEVELPDESVVTAKIPPGTQPGTVISVSGKGMPRIDRSGRGSLHVMIDVV
                     VPAKLSKRAKKLLQELDEELSTDGTEAHAQ"
     gene            complement(1802..3145)
                     /locus_tag="VFQ35_15980"
     CDS             complement(1802..3145)
                     /locus_tag="VFQ35_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932203.1"
                     /translation="MTTVRSIAESIRRTYVGSLSLMALALVAVTVASSAYVAIQRDDA
                     ATLSQVQILDGELRESREASLEEQDEHVRHEMAEQEGSPHEMEIWKGARRLGWPGQSN
                     QPPFWRMTRGCSTSHARGGWERVCAFEIGGGMTLVSATGLRPLLAKQLPIWGAVAVCT
                     GLAVLALALLSRRVVEASLTPLASFEREMAKLPAETGARLPTAWSATEIDQLARTFNS
                     LLARIDEMVEREQTFASNAAHELRTPLTRLRGQLGLLLEEPAVRGEIRERLERAQLTC
                     VELVDCTEALLALSHRVASLDEAVDLAEVALTVRNGLREQDVKRVELSASEAIVRGDT
                     ALVRLAVQNLVENALKYSDGRIDVEVRSDDDECLLMVADAGPGIPEAELAVVREPFQR
                     GSKRRGDVRGTGLGLALVDHVASLHGGRLELANRVAPRGLLASLHLPPWSPRKAA"
     gene            complement(3142..3816)
                     /locus_tag="VFQ35_15985"
     CDS             complement(3142..3816)
                     /locus_tag="VFQ35_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9932204.1"
                     /translation="MRVLIVDDDEETLEVVGRALERDAHVVSFATTAEQAWQALESNA
                     IDVIVLDVMLGQSSGLELCAQIRRRGLSIPILFLSARGAVNARVEGLEAGGDDYLPKP
                     FAVRELLARVRALGRRQPTKRAVQISFGRLTLNFDTRRALADEQEIPITRREWDILRA
                     LADADGRVVSFDEVLEQAWGDVSERGRASLEVIVSRLRRKLSAAAGRQLIRTVRGAGY
                     ALEVDT"
     gene            4107..4811
                     /locus_tag="VFQ35_15990"
     CDS             4107..4811
                     /locus_tag="VFQ35_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9932205.1"
                     /translation="MARILIIEAEATTRQLLAYDLGAAGHEVITSATAADGLQIARDT
                     QPDLILSELTLPDGSGTDVCGVLRQEAGIGETLFMILSAQSDEVDRVRAFENGVDDYL
                     TKPFSPRELVLRIRAMLRRSLRSHAQSTITNIGCLRLDTAAYRVWVDGQEVHLTTTEF
                     QILLALHENFGRVLTREKLIESLWNEGEIDIRTVDAHVRRLRLRLGAAANYIKTVRGV
                     GYRLSYERAVQAAGRA"
BASE COUNT          892 a         1636 c         1604 g          777 t
ORIGIN      
        1 gctgccgaga cctcggccgc cgctatcctc accaaaggcc tcgagcagaa gctcctcagg
       61 caagaggcct ggatcaacga gctcgaggcg cgggccgcaa ccgccgatga gcgagccgac
      121 gcagctgaat cagagctcga cgaggtccgc gagcggctcg gtgagctcga gcaacgtcac
      181 gagcgcgagg ccacggcgct cactcaagaa cgcgacgcgc tcaaggccga gctcgagcgc
      241 ttgcgcaagc gcgtgaacga ccaggacgac ctcatcgcct tgaaagacac cgagctcgcc
      301 gccctggccg acgacggcga ggcgacccgt gaacttgcac ggctcgaggc gcagctgaaa
      361 gaacgcggag agcgcgtcct cagcctggag cgcgaagtgt tcgaggccga gcggatcggg
      421 cgcgagcttt tacgcaagct ggatacgagc gccggggccg cctcccagca gctcgccgag
      481 cagctcgccc gagcggaagc ggagctactc acgctgcgct ggtccctgga tctagccaag
      541 cgcccggcgc acaacggcgt accgcagtcg tgaacggagc tcgctcggac gcgttgacag
      601 gcttgccgca ggccttagct tgcagacaca tctctgcgac tcgggcgaat tgaagcatga
      661 gtgaaaaacg ggattactac gaagtcttag ggttatcgca gggatgcagc tccgacgaga
      721 tccggaaggc ctacaagcag tgcgcgctga agtaccaccc ggatcgcaac cccggggata
      781 agtcggctga agcgaagttc aaagaggcga ccgaggcgta cacggtcctc tcggacgacg
      841 acaagcgacg tacctacgac cgcttcgggc acgcgggcct cgacggggcg ggcgtcgact
      901 tctcgggcgc cggcgtgaac gacatcctga accactttca ggacatgttc atggacttct
      961 tcggcgggat gggtgggccg caacagggcc gccgacgccg agccgagcgc gggcaagacg
     1021 tcggtgttgc cgtggagctg acgctcgcgg agtccatgct cggcggcaaa cgcgaggtca
     1081 ccgtacgggg acctgccgcc tgcgagacgt gccacggcaa gggaacgaag tcgggcagcg
     1141 cgcctgccaa gtgccaggct tgtcgcggca ccggtcaggt gacagcgcag cgcggcttca
     1201 tcatgttcgc gtcgccgtgc gcccgctgcg gcggtagcgg tacgttcatt caggacccgt
     1261 gcgagacgtg caatggcgcc gggtcggtcg agcggcagcg ccgcgtgctc gtcacgtttc
     1321 cggccggcat cgactctggg cagcgcttgc gtgtgccggg tcagggcatg cccggtcccg
     1381 aggggacgcc cgcgggagat ctgtacgtcg acgtcgagct taccgcgcac ccccagttcg
     1441 aacggcacgg cgatagtttg gggacgaggc gtcgcgtctc gttttcgacg gccgcgctcg
     1501 gcggcgaggt cgaagtcgag ctccccgacg aaagcgtcgt cacggccaag atccctcccg
     1561 gtacgcaacc gggaacggtg atctccgtct ccggaaaagg catgccgcgc atcgatcgct
     1621 ctggccgagg cagccttcac gtcatgatcg acgtggtggt gcccgcgaag ctctcgaagc
     1681 gcgcgaagaa gctcttgcaa gagctcgacg aagagctctc gaccgacggc accgaagccc
     1741 acgcccagta agggcggtct ttgcggccaa gttgccgccc cactcaccga cgagctcgcg
     1801 ctcacgccgc cttgcgcggc gaccatggcg gcaagtggag cgacgcgagg aggccgcggg
     1861 gggctacacg gttcgcaagt tccaggcgcc caccgtgcaa cgaagcgacg tggtcgacga
     1921 gcgcgaggcc gagccctgtg ccgcgcacgt cgccgcgacg cttgcttccg cgttggaagg
     1981 gctcgcgcac gacggcgagc tctgcctccg gaattccggg gccggcgtcc gcgaccatca
     2041 agaggcattc atcgtcgtcg gaccgaacct cgacgtcgat gcggccatcg ctgtacttga
     2101 gcgcgttctc gaccaggttt tgcaccgcca agcgaacgag cgccgtatcg ccgcgcacga
     2161 tggcttcgct cgcagaaagc tcgacgcgct tcacgtcctg ttcacgcagc ccgttgcgca
     2221 cggtgagcgc cacttcggcc aagtcgacgg cttcgtccaa ggaggcgacg cgatgagaca
     2281 acgcgagcag ggcctcggtg cagtcgacga gctcgacgca cgtgagctgc gcgcgctcga
     2341 ggcgctcgcg gatctcacct cgtaccgcgg gctcttcgag cagcaagccg agctggccac
     2401 gtaagcgagt gaggggcgtc cggagctcat gcgcggcgtt cgatgcgaag gtctgctctc
     2461 gctcgaccat ctcgtcgatg cgcgccagga gagagttgaa ggtcctcgcg agctgatcga
     2521 tttcggtggc gctccacgcg gtcggaaggc gcgcgccggt ctccgcaggc aacttcgcca
     2581 tctctcgctc gaagctcgcg agcggggtga ggctcgcctc gacgacgcga cggctcagca
     2641 gcgcgagcgc gaggacggcg aggccggtgc aaacagccac tgctccccag atcggaagct
     2701 gcttcgcgag cagcggccgg agccccgtag cggacaccag tgtcatgcca ccgccgattt
     2761 cgaacgcgca cacgcgctcc cagccgccgc gcgcgtggct cgtcgagcag ccgcgcgtca
     2821 ttcgccaaaa tggcggctga ttgctctgac cgggccaccc gagccggcgc gcgcccttcc
     2881 agatttccat ctcgtgggga gagccttctt gctccgccat ttcgtggcgc acgtgctcgt
     2941 cctgctcctc gagcgaagct tcgcggctct cgcgaagttc gccgtcgaga atttggacct
     3001 gcgacagcgt ggccgcgtcg tcgcgttgaa ttgcgacgta cgcggaagac gctaccgtca
     3061 ctgcgacgag cgcgagcgcc atcaacgaga gggagccgac gtacgtccgt cggatcgact
     3121 cggcgatgga ccgcaccgtc gtcacgtgtc gacctcgagc gcatagccgg cgccgcgcac
     3181 ggtgcggatc aactgtcgcc ccgccgccgc cgaaagcttt cggcggagcc gcgaaacgat
     3241 cacctcgagg ctcgcgcggc cgcgctccga gacgtccccc caagcctgct ccaagacttc
     3301 gtcgaacgaa acgacgcggc catctgcgtc ggcgagagcg cgcaaaatgt cccattcgcg
     3361 gcgggtgatg gggatttctt gctcgtccgc gagcgctcgg cgcgtatcga agttcagtgt
     3421 aagtcgcccg aaagaaatct gcaccgcgcg tttcgtcggc tggcgacgac ccaacgcacg
     3481 cacgcgcgcc aagagctctc gcaccgcaaa cggcttcggc aaatagtcgt caccgcccgc
     3541 ttcgagaccc tcgacgcgcg cgttgacggc gccgcgcgcg ctcaagaaca atatggggat
     3601 agagaggccg cggcgacgaa tctgcgcgca caattcgagc cccgagctct ggcccaacat
     3661 cacgtcgagc acgatgacat cgatggcgtt gctctcgagc gcctgccacg cttgctcggc
     3721 cgtcgtcgcg aacgaaacca catgcgcgtc gcgttccaat gcgcgcccga cgacctccaa
     3781 agtttcttca tcatcgtcca cgatcagcac gcgcatgggc acgatattgc ctcagctcga
     3841 cgctcgagcg cgaggtgacg cccccccgct cacgagaccc gtgggctgaa tcggccttaa
     3901 ccggagctta acgggggctc gcgccgaact catttccgcg tattctttcg cgaggcagct
     3961 ctcccgagcc gagctcgtcg cgtacccaac cccgtcgagc tggtggggaa tcatcggtaa
     4021 cgcgagccgt tccccaagtc gaccaaaatc aaattgatgc tctttgggca tcatcggaat
     4081 cccggcgcgg agcagccgtt tcagagatgg ctcgaattct catcatcgag gctgaagcaa
     4141 ccacccgtca attgctggca tacgaccttg gtgccgctgg ccacgaggtg atcacctcgg
     4201 ccactgcggc cgacggcctg cagattgcgc gggataccca accggatctc atcttgagcg
     4261 agctcaccct tccggatggc tccggtacgg acgtgtgcgg cgtgcttcgg caagaagcag
     4321 ggatcggcga gacgctgttc atgatcttga gcgcgcagag cgacgaagtc gaccgggttc
     4381 gcgcgttcga gaatggcgtg gacgattacc tcacgaagcc cttcagcccg cgagagctcg
     4441 tgctgcgcat tcgggcaatg cttcgtcgtt ccctccgctc ccacgcccag tcgaccatca
     4501 cgaacatcgg ctgcctgcgg ctcgacacgg cggcctatcg cgtctgggta gatggccaag
     4561 aggtgcacct gacgaccacc gagttccaaa tccttttggc gctacacgaa aatttcggtc
     4621 gcgtgctcac tcgcgagaag ctcatcgaaa gcttgtggaa cgagggagag atcgacattc
     4681 ggaccgtcga tgcccacgtg cggcgcttgc ggctccgtct gggcgcagcc gcgaactaca
     4741 tcaagaccgt gcggggcgta ggctaccggc tctcttacga gcgagccgtg caggccgcgg
     4801 gcagggccta agagccaata gctccgggtt ggttctcgtc ttcaacgctt cgcggcgggg
     4861 cgcgcgccga gcgctcgaga gagccgcacg acctcgctct tggtgcgct
//
LOCUS       DASTKS010000770         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1685348, whole genome shotgun sequence.
ACCESSION   DASTKS010000770 DASTKS010000000
VERSION     DASTKS010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685348"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2339)
                     /locus_tag="VFQ35_15995"
     CDS             complement(<1..>2339)
                     /locus_tag="VFQ35_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932206.1"
                     /translation="RVELALGEHLFAVPYTKNALPQQPFDGTTLEQLMADGPAAIQGR
                     VVHLIEVEVVDRAAQHLDWEKWLRAGMTRPIAVVRKSVVRPRALDVPLTYPLRWLTLR
                     SQGPDRYGPPLKSEFDLVFSVADAREAVIVDDAEEDEVIAFRRTVAWPTAEVIHFDHP
                     PLMNVEALFDLTASWQTRLVLFETPTPTELAETAGRLADLGGPAVLIVKPGLRFPLCL
                     SLVHDEPLDWLAFQLASEDLILVGGAGREDLLRVSRIARMLESTELDRTIQEQLQTAP
                     NDAELELGFAGGTPADTSFLSSFRSVEARTLSELSFDQERRGTLPSAHLVRKLQAAVR
                     SARTPTPPLRAPTEANERRHVNAVLHHVSERERSLLDSAYGWVEPLQELELAVQIGAR
                     IEGLRTIGSAAFLEEAVLWDADAQQTILEVGVTSLDFELFGAPVQRIALPRTGDSAIA
                     TFRLKAKAETRIPGVARLRFCLYHGNTLIQSFRVAVAHGKQPPDGEELQNAFQRALDL
                     SHEHAALLRGVAGGYFARLEYSAFDLEHIRDFKPRRVSFFVNDSEGEVVVTTKGDEIF
                     SGEISPNLTGYVSEARNTLKNSSYALDAEGGLIPNVYRFFNEKKLNVGDEVAYDAALC
                     DLAAAGFDLYSALVPRDDQAKFERALSEPRQIVHAGHLKLDGVVPWNLLYLRQFDDKS
                     GKPTKACSAPVEGSRCGDHPDCPLHTSNAARGFTDTTTACPRYFLGFRQLVEVPPQQV
                     QGIKQTLAEQSETASSMNRVIVCGPKVRTLVGYNPALRL"
BASE COUNT          401 a          714 c          789 g          435 t
ORIGIN      
        1 gaggcgaaga gctggattgt acccgaccag cgtgcggact ttgggcccgc agacgatgac
       61 gcggttcatc gagctcgcgg tttcactctg ctcggcgagt gtctgtttga tgccctgtac
      121 ttgctgcggt ggtacctcca cgagctgccg aaacccgagg aagtagcgcg ggcacgcggt
      181 ggtcgtgtcc gtgaagccgc gggccgcatt cgaagtgtgc agcgggcagt cggggtggtc
      241 tccgcagcgc gagccttcga ccggcgccga gcaagccttc gtcggtttgc cgctcttgtc
      301 gtcgaactga cgcaggtaga gcaagttcca gggaacgact ccatcgagct tcaaatgtcc
      361 tgcgtgtacg atttgccttg gctcgctgag ggcgcgttcg aacttcgcct ggtcgtctcg
      421 gggaaccaga gccgaataga ggtcgaagcc agctgccgct aggtcgcaga gggcagcgtc
      481 gtatgcaacc tcgtcgccga cgttgagctt cttctcgttg aagaagcggt aaacgttcgg
      541 aatcaagccg ccttccgcgt cgagagcata ggaagagttt ttgagagtgt tgcgcgcttc
      601 ggatacgtac ccggtgaggt ttggcgagat ctctccggag aagatctcgt cccccttcgt
      661 ggtcacgacg acctcgccct cgctgtcgtt gacgaagaag ctgacgcgcc gtggcttgaa
      721 gtcgcggatg tgttcgaggt cgaacgccga gtactcgagg cgcgcgaagt agccaccggc
      781 gacgccgcgg agtagcgccg cgtgctcgtg ggacagatcg agcgcgcgct ggaaggcgtt
      841 ctgtagctct tcgccatccg gcggttgctt cccgtgggcg acggccacgc gaaacgactg
      901 gatcagcgtg ttgccatggt agaggcaaaa acggagccga gctacgcccg gaatcctcgt
      961 ctccgccttt gccttgagtc gaaacgtcgc gatcgcgctg tcgccggttc gtggcaacgc
     1021 gatgcgctgc acgggcgcgc cgaagagctc gaagtcgagt gacgtgacgc cgacctcaag
     1081 gatcgtctgc tgcgcgtcag cgtcccacag caccgcctct tcgaggaaag cggcgctgcc
     1141 gatcgttcgg aggccctcga tgcgcgcccc gatctggacg gcgagctcga gttcctgcag
     1201 cggctcgacc cagccatagg cgctgtcgag cagggagcgt tcccgctcgc tcacgtgatg
     1261 caagaccgcg ttcacgtgtc ggcgttcatt cgcttcggtc ggggcgcgga gaggaggagt
     1321 tggtgtgcgc gccgagcgaa cggcggcttg cagctttcgc acgaggtgcg ccgaggggag
     1381 ggtgccgcgg cgctcttggt cgaagctcag ctccgacagc gttcgagctt cgacgctccg
     1441 gaaactcgag aggaaactcg tatcggcagg cgtcccgccc gcgaagccga gctcgagctc
     1501 cgcgtcgttc ggcgcagttt gaagctgctc ctggatggtt cggtcgagct ccgtgctctc
     1561 cagcattcga gcgatgcgcg acactcgcag caggtcttca cgtcctgccc cgccgacgag
     1621 gatgaggtcc tcgctcgcga gctggaacgc cagccaatcc agcggctcgt cgtgaacgag
     1681 cgataaacac agcgggaagc gaagcccagg tttgacgatg agcaccgcgg ggccgcccag
     1741 atccgcgagc cgccccgcgg tctcggcgag ctccgtcggg gttggcgttt cgaataagac
     1801 gaggcgggtc tgccaacttg cggtgaggtc gaagagcgct tcgacgttca ttagaggcgg
     1861 atggtcgaaa tggatgacct ccgccgtcgg ccaagcaacg gttcgcctga acgcgatgac
     1921 ctcgtcttct tccgcgtcgt cgacgattac cgcttccctc gcgtccgcga cggagaacac
     1981 gaggtcgaac tcgcttttca gcggcggccc gtaacggtcc gggccctgcg atcgcaaggt
     2041 gagccagcgc aacggatagg tgagcggaac gtcgagcgcg cgtgggcgca cgacgctctt
     2101 ccgcaccact gcgatggggc gagtcattcc agcgcgcagc cacttctccc aatcgaggtg
     2161 ttgcgcggcg cgatccacga cttcgacttc gatgaggtgg acgacgcgcc cctggatcgc
     2221 cgctggccca tctgccatca gctgttcgag cgtggttccg tcgaaaggct gctgcggtaa
     2281 ggcattcttc gtataaggga cagcgaacag atgctctccg agggcgagct cgactcggc
//
LOCUS       DASTKS010000771         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1463236, whole genome shotgun sequence.
ACCESSION   DASTKS010000771 DASTKS010000000
VERSION     DASTKS010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463236"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFQ35_16000"
     CDS             <1..441
                     /locus_tag="VFQ35_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9932207.1"
                     /translation="AHSQATSVHFFGLPISVHRAIEPALRCVEKRIRATCQRGADHYT
                     PRAIGGFRDRNTYRGGEVSNHMFGIAIDIDPERNPCCGCVEPWPQHPACQTHGANAVE
                     SAALPKCWIEGFERYGFYWLGRDPQLRDTMHFEFLGNPERVVAR"
     gene            complement(471..1577)
                     /gene="ald"
                     /locus_tag="VFQ35_16005"
     CDS             complement(471..1577)
                     /gene="ald"
                     /locus_tag="VFQ35_16005"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9932208.1"
                     /translation="MIVGIPKETKLHEYRTGMLPVGVQELTRQGHRVLVQRAAGLGSG
                     IRDAEYEQAGAMMVDDPFEIYGAADLIVKVKEPQPAEIPLLRSGQLVFTYFHFAASES
                     LTMGVLASGATAIAYETLSDQRGNLPLLTPMSEVAGRMSIQQGAKFLERPQEGRGILL
                     GGVPGVEPAHITILGGGVVGTNAAKIAAGFGASVCVLDTNLDRLRYLDDIMPHNVTTL
                     FSDRYTILDQLAKADLVVGAVLLRGARAPRLVRREDLKQMKPGAVVVDVAVDQGGCFE
                     TTRPTTHAEPTYVVDDVVHYAVANMPGAVGRTSTYALCNATLPYALLLAKHGLDEAAR
                     QRSELVSAVNVHAGKVTHPAVAETFGLEYTPFSG"
     gene            complement(1596..2228)
                     /locus_tag="VFQ35_16010"
     CDS             complement(1596..2228)
                     /locus_tag="VFQ35_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9932209.1"
                     /translation="MQLIGQYDSPFVRRVAIALTLYELPFEHLPLSVWADAEELAKSN
                     PLRRVPTLVLDTGECLIESFAILDALDEMVGPRRALIAAAGVERRNALRICALATGSA
                     DKAVSLFYEKLLRPEPSEKWLSRCRAQIGDALSLLNRERERIATSYWFGESLNHADIA
                     VACALRFINDALPGAFDMRRFPALARHSAQCEALPVFQAIAQPFEVMLSR"
     gene            2316..>2843
                     /locus_tag="VFQ35_16015"
     CDS             2316..>2843
                     /locus_tag="VFQ35_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9932210.1"
                     /translation="MRDPDAIVIGSGPNGLTAAVELARAGLAVLVLEANPRRPGGSVG
                     SDEGTLPGFVHDYGAAFFPMARVSPAFRSLGLERHGVTWLNADVESCHPALDGSSASI
                     VRLESDAPRRPDYFGSVPDTLLWERLAREHAREERRLFDSLLAPLPDFGFPFRLGLLR
                     SLRLATRFAVSTAGLS"
BASE COUNT          469 a          926 c          943 g          505 t
ORIGIN      
        1 gcgcactccc aggccacttc ggtacacttc ttcgggctac cgatcagcgt tcatcgagcc
       61 atcgagccgg cgctgcgctg cgtggagaag cgcatccgag cgacgtgtca gcgcggcgca
      121 gatcattaca cgccgcgcgc gatcggcggc tttcgtgatc ggaacacgta ccgcggcggc
      181 gaggtctcga accacatgtt cggcatcgcg atcgacatcg atcccgagcg caacccgtgc
      241 tgcggatgcg tcgagccatg gccgcagcac cccgcttgtc aaacgcacgg cgcgaacgcg
      301 gtcgagagcg cggcgctacc caaatgttgg atcgaagggt tcgaacgcta cggcttttat
      361 tggctcggcc gcgatccgca gctccgcgac acgatgcatt tcgagttctt gggaaatccg
      421 gagcgcgtcg tcgcgcgctg acgcgctccc atcggcaacc ggcctcgtcg tcagccgctg
      481 aatggcgtgt attcgaggcc gaacgtctcg gccacggcgg ggtgagtgac cttgccggcg
      541 tggacgttga cggcactgac gagctccgag cgctgacgcg cggcctcgtc gagaccgtgc
      601 ttcgcgagca ggagcgcgta aggcagcgtg gcgttgcaga gcgcgtacgt gctcgtcctg
      661 ccgacggcgc cgggcatgtt cgcgaccgcg tagtgcacga cgtcgtcgac cacgtacgtc
      721 ggttcggcgt gcgtcgtcgg gcgcgtcgtt tcgaagcagc cgccttggtc gacggcgacg
      781 tcgacgacca ccgcgcccgg cttcatctgc ttcaaatctt cgcgacgtac gagccgcggc
      841 gcgcgcgctc cacggagcaa cacggccccg acgacgagat ccgctttcgc gagctgatcc
      901 aagatcgtgt agcgatcact gaacaacgtc gtcacgttgt gcggcatgat gtcgtcgagg
      961 taccggaggc ggtcgaggtt cgtgtcgagc acgcagacgg acgcgccgaa gccggccgca
     1021 attttcgcgg cgttggtacc gacgacgccg ccgcccaaaa tggtgatgtg cgcaggctcc
     1081 acgccgggca cgccgccgag caagatgcct cgaccctctt gcggccgctc caagaacttg
     1141 gctccctgct ggatgctcat gcgcccggcg acttcgctca tgggcgtgag cagcggtaag
     1201 ttcccgcgct gatccgagag cgtctcgtaa gcgatcgcgg tcgcgcccga ggcgagcacc
     1261 cccatcgtca gcgactcgct cgccgcgaaa tgaaaatagg tgaaaacgag ctggccgctt
     1321 cgcaaaagcg gtatttcggc gggctgcggc tccttcactt tgacgattag gtcagcagcc
     1381 ccgtagatct cgaacgggtc gtcgaccatc atcgcgccgg cttgctcgta ctcggcgtca
     1441 cgaatgccgc tgccgagccc ggcggcgcgt tgcacgagca cgcggtgccc ttgacgcgtc
     1501 agttcttgaa cgccgaccgg gagcatcccg gtacggtact cgtgcagctt ggtttctttg
     1561 ggaatgccga cgatcacagg aactctcctc tgcgctcagc gcgaaagcat gacttcgaat
     1621 ggttgggcga tggcttggaa gacgggtagc gcctcgcatt gggcggagtg gcgcgcgagc
     1681 gccggaaacc gcctcatgtc gaaggcgccg ggaagcgcgt cgttgatgaa gcgcagcgcg
     1741 cacgcgaccg cgatgtcggc atggttcaag ctttcgccga accaatacga agtcgcgatg
     1801 cgctcgcgct cgcggttcaa gagcgagagc gcgtcgccga tctgcgcgcg gcagcgcgaa
     1861 agccacttct ccgacggctc cggcctgagc agcttctcgt aaaataagct caccgccttg
     1921 tcggcactgc ccgtcgccag cgcgcaaatg cgcagcgcgt tgcggcgctc gacgcccgcg
     1981 gcggcgatca aagcgcgccg ggggcccacc atctcgtcga gagcgtcgag gatcgcgaag
     2041 ctttcgatca ggcattctcc cgtgtcgagg acgagcgtcg ggacacgccg cagcgggttg
     2101 ctcttcgcga gctcctccgc gtctgcccag acggaaaggg gcaggtgctc gaaaggaagc
     2161 tcgtagagcg tcagcgcgat cgcgacccgc cgcacgaacg gggaatcgta ttggccgatc
     2221 agctgcatct ggttacaata ccgccgacat gcacgcggag cgagatgccg tgtcgagcgc
     2281 gacttcttcg cctccacggc gccgccttgt ttcggatgcg cgatcctgat gccatcgtaa
     2341 tcgggtctgg gccgaacggg ctcaccgccg cagtcgagct cgcgcgcgcg ggtctggcgg
     2401 tgctcgtgct cgaggcgaat ccgcggcggc ccggcggttc ggtcggaagc gacgagggga
     2461 ctctacccgg gttcgtccat gactacggcg ccgcgttttt tccgatggcg cgcgtaagtc
     2521 ctgcgtttcg aagcctcggg ctcgaacgac atggagtgac ttggctcaac gcggacgtgg
     2581 agagttgcca tcccgcgctc gacggaagct ccgcgtcgat cgtccggctc gaaagcgacg
     2641 cgccgcgaag gcccgattat ttcggttcgg ttccagacac gcttctgtgg gagcgtctag
     2701 cgcgcgaaca cgcgcgcgaa gagcggcggc tcttcgactc gctcctggct ccgctcccgg
     2761 attttgggtt tccgtttcgc ttgggactgc tgcgctcact gcgcctcgcc acgcggtttg
     2821 cagtctccac ggcagggctt tcg
//
LOCUS       DASTKS010000772         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1910123, whole genome shotgun sequence.
ACCESSION   DASTKS010000772 DASTKS010000000
VERSION     DASTKS010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910123"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VFQ35_16020"
     CDS             complement(<1..1021)
                     /locus_tag="VFQ35_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932211.1"
                     /translation="MKQETEDFLADHGLGRLERRLRLGGRLKSGAGPELPAELCLTPT
                     GAWVVACDGRFVGEAIDAGDAEKVRYHTGALTDRLSVGEQFLAVPPGKGKEARRLIAL
                     GRLRRRRLGNEPATLPLGGDRYLTDEGEVSRELVAAVVASPDQLIALVELDATEQASA
                     LGPEVDAVNYFVLSCDKAWFVALSELGDLATEELDARTLCVEAKSDRTVLTDGNRSRA
                     VPGKRRSLVQELVELGQHAGAERACEAARRLHVSGRAPRARTLLAEAGRRGEPRATLL
                     GIALDIELGATHVPRDEYVAAVARLRDGTVTGDACGDTFRRWRFRLAVGREVVHALTL
                     LGGEAE"
     gene            1151..>2012
                     /locus_tag="VFQ35_16025"
     CDS             1151..>2012
                     /locus_tag="VFQ35_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9932212.1"
                     /translation="MTTPHTSSEIRRAFLEFFRGKDHEVLPSGSLIPANDPTLMFANA
                     GMVQFKDVFTGREKRPYRRATTSQKCIRISGKHNDLEAVGPSPRHNTFFEMLGNFSFG
                     DYFKEEAIVYAWEFLTKVAGIPPERLAVTYFAGEQGFAEDAQARDIWKKVTGFGDDCV
                     RPLGMADNFWSMGDTGPCGPCSEIYFYNGSSDVRVEAFGEEQTAGGVGWMEIWNLVFM
                     QFERSIDSSGQTHTTALPAPSIDTGMGLERLACVLQGKSIAYDTDILRDLVEQAARLS
                     NKTFHGSADPD"
BASE COUNT          341 a          704 c          646 g          321 t
ORIGIN      
        1 gctcggcctc tcctccgagt aacgtgagcg cgtgaacgac ctctcgcccc accgccaggc
       61 ggaagcgcca acggcgaaac gtgtcaccac aggcgtcgcc ggtcaccgtt ccgtcgcgta
      121 agcgcgccac tgcggcgacg tattcgtcgc gcggcacgtg cgttgcgccg agctcgatgt
      181 ccaaagcgat cccgagcaag gtggctcgag gctctccccg gcgtcccgcc tcggcgagca
      241 acgtgcgcgc tcgcggcgcg cgcccactca cgtgcaatcg gcgagcggcc tcgcaggctc
      301 gctcggcgcc agcgtgctgc ccgagctcga cgagctcctg gaccaggctg cgtcgcttcc
      361 ccggtaccgc acgggagcga ttgccgtcgg tgagcacggt ccggtcgctt ttcgcctcca
      421 cgcagagcgt tcgcgcgtcg agctcttcgg tggccaagtc gccgagctcg ctgagcgcca
      481 cgaaccaggc cttgtcgcag ctcagcacga agtagttgac ggcgtcgacc tcgggtccca
      541 gcgcggacgc ctgctcggtc gcatcgagct cgacgagcgc gatgagctga tcgggcgaag
      601 cgacgacggc tgcgacgagc tcacgagaaa cctcgccttc gtccgtcaag tatctgtcgc
      661 cgccgagcgg cagcgtagcc ggctcgttgc cgaggcgccg tcgccgcagc ctaccgagcg
      721 cgatcagccg tcgagcctcc ttccccttac cgggcgggac ggcgaggaat tgctcgccca
      781 cgctgaggcg gtcggtgaga gcgccggtgt gatagcggac cttttcggcg tctccggcgt
      841 cgatagcctc cccgacgaac cgaccgtcgc acgcgaccac ccaggctccc gtcggggtga
      901 gacacagttc ggcgggtagc tctggccccg cgccggactt cagcctaccg ccgagccgaa
      961 ggcgccgttc gaggcgaccc aggccatgat cggccagaaa gtcctcggtt tcctgcttca
     1021 cgagcgccct ccgaacgggc tgcgggggct cgccgaacga gccccgccgg gcgttttccg
     1081 ttttcgatga agcggctccg gctcgacgct gcccgcctcg gggtgagtaa tgctatgggt
     1141 cacgccgacc atgaccacgc cccacacgtc atccgagatt cgccgcgcgt tcctcgagtt
     1201 cttccgcggc aaggaccatg aagtgctgcc gagcggctcg ctgattcccg ccaacgaccc
     1261 gactctgatg ttcgccaacg ccggcatggt tcagttcaag gacgtgttca ccggccgtga
     1321 gaagcgccca tatcggcgcg ccacgacgag tcagaagtgt atccgcatca gcggcaagca
     1381 caacgacctc gaagcggtag gcccctcgcc ccgtcacaac accttcttcg agatgctcgg
     1441 aaacttcagc ttcggcgact acttcaaaga agaagccatc gtctacgcat gggagttcct
     1501 gaccaaggtc gccgggatcc cgccggagcg cctggcggtg acttacttcg ccggagagca
     1561 aggcttcgcg gaggacgccc aggcgcgcga catctggaaa aaggtcaccg gctttggtga
     1621 cgactgcgtt cgcccgctcg gcatggccga caacttctgg tcgatgggcg acacggggcc
     1681 gtgcggaccc tgcagcgaaa tctacttcta caacggcagc agcgacgtgc gcgtcgaggc
     1741 tttcggcgag gagcaaaccg cgggcggcgt tggctggatg gagatctgga acctggtctt
     1801 catgcagttc gaacgcagca tcgactcgag cggccagacg cacacgaccg cgttgccggc
     1861 tcccagcatc gacaccggca tggggctcga gcgcctcgcg tgtgtgctgc agggcaaatc
     1921 gatcgcgtac gacaccgaca tcctgcgtga cctcgtcgag caggcggctc gcctcagcaa
     1981 caagacgttc cacggcagcg cggaccccga cg
//
LOCUS       DASTKS010000773         6216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_569853, whole genome shotgun sequence.
ACCESSION   DASTKS010000773 DASTKS010000000
VERSION     DASTKS010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569853"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFQ35_16030"
     CDS             complement(<1..645)
                     /locus_tag="VFQ35_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9932213.1"
                     /translation="MNKGTAIVGFIMSFIAGVFLMWGVERSRGGSDIQAESASGSGAV
                     ADQSAASVPVSAKDPQKGKADAPVTIVEISDFQCPFCSRVTPTIKQVEETYKDQVRVV
                     FKHNPLPFHNRARPAAEAANTVFALGGSQAFWKFHDQAFGNQQNLTDENFENWAAAAG
                     VDKAKFKEAYGAKKYAAKVDEDMALAQKINANGTPAFRINGVTVSGAQPFDKFKE"
     gene            complement(784..1173)
                     /locus_tag="VFQ35_16035"
     CDS             complement(784..1173)
                     /locus_tag="VFQ35_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932214.1"
                     /translation="MARLFAVVLLTLLVFGTRRAHAMSLVPMCGVNAETVVAPPISRA
                     AQEIPLGPSACDPQSDPRLETTAPRQTPPELPSIEVVPRVPPVAFHLPREPRTRAPLV
                     PIQDGERSAHRSALERPPRRLAAIQLA"
     gene            1353..4070
                     /locus_tag="VFQ35_16040"
     CDS             1353..4070
                     /locus_tag="VFQ35_16040"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9932215.1"
                     /translation="MQALELSENAPKSLENQASTAAPAEARPEPLPRSFERFTLLSRV
                     ARGGMGEVYLAAARSIEGAERPLIVKIIRPDHADDRSFVARFLDEARIQAQLQHPGVA
                     QIIEATTDQTGKPYVVVEYVEGRNLSDVRARAGQLGVRVSWPEALALGVCMGEALAHI
                     HERTDPEGRPLEIVHRDLSPQNVMVGFGGDLKLIDFGTARGQNRRCQTIAGVVFAKPG
                     YVAPEVANNSPGGIPADLYAFGVILWELLAGRRFLTGEATAHMVAVGSGKRTLPPLAP
                     IVGAPPEIDQILAQLTATKIEDRYASARVATQDLVKLLQRAPSLADGDRSVRGRISDL
                     MRRLYPAEPTRSRADFARRVAEARSVAPRVVAPPPSPEPPESTDSSVLDGTRYRLGRL
                     IGRGGMGAVHEAVHLDLGRTVALKLLESDTMGEKAQSRFRAEARAIAQLSHDNIVRVY
                     DFGACRDGRLFYAMELLDGETLAMRLLSVPHLPFASVVDFGIQACRALEAAHAASVVH
                     RDLKPANLFVTQTGTLKVLDFGVAKSAAASEAAEKDAITIVGTPEYMAPEQLKGTADA
                     RSDLYALGVVLFELATGTLPFEAEGPLEILSRKNSEVAPRVRERGAEPLPPAFDSLLA
                     RLLERDPARRPESARALRQELEALLTAPRSRQTLRRKFGSVAVGASLFIGAAVLANAG
                     MRNTQASSALRSSLGEVTARVMSFGEQLKHRREALLAARTASSAVAQSQLAAQPEAKI
                     VAKQPSEPAPVTEASPKEPEIIDADALANEVSAEPVRAPEVSSETAQALAEAKALTDK
                     GRELKALDVLRRAVERHPDDVELLGALSQALSKNRSWGEALRVARHRAELDQSAPARF
                     DLARLERATGHRDRAIELLNGLLGDDQHGSAARELLKSLNGASRVALKD"
     gene            4208..5647
                     /locus_tag="VFQ35_16045"
     CDS             4208..5647
                     /locus_tag="VFQ35_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9932216.1"
                     /translation="MALPPSASSASVATDGSPYRVVLRLATPTILAMLAQSAVNEIDI
                     IFFARLPCPESSNAQAALLPSLILLWMFGGSLSAISVGTQAIAARRFAERKQLDAGAV
                     LVNSWVFSLVAGIAFTLLGYLAMPSVLNLLIKVPEVREAARTYLGYRLLGITSMAMTF
                     SFKAFFDGIGRTHVHMIASVVMNILNVLLCIAFIFGNWGAPRMGIGGAGLAGFVSTWV
                     GLAIMIAWALHSRYRSQYNPFSLSRVSKRGVWDILRLSIPSAIATIAVMTGFALFSTI
                     VSYLDSVSHAAVLSPDCPGGKAQAVNSAATMVIVGILKLTFTACLAFGTSTATLVSQS
                     LGEGSPERATRFGWVSVRLGLMIFGVVGLIEGVLFTNQILGFVTHSEAVLAAASGPMR
                     LMGICTPLIATGMILTQALFGAGNSRFVMIVELVLHFGCLVPLAWLLGVTLNFGLPGI
                     WGAATVYVVLLTAIMVMKFRSGDWQAIRL"
     gene            5938..>6216
                     /locus_tag="VFQ35_16050"
     CDS             5938..>6216
                     /locus_tag="VFQ35_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9932217.1"
                     /translation="MSSVRIHELLARRLTTHAFDPEERVSRADCSALLRAAHAAPSWN
                     NQQPWRFLVCDRFERPDAWQSAFDALERKERPWAYNAALFIVVAVDLDD"
BASE COUNT          967 a         2053 c         2063 g         1133 t
ORIGIN      
        1 ctccttgaac ttgtcgaagg gctgcgcgcc gctgaccgtg acgccgttga tgcggaacgc
       61 gggggtcccg ttggcgttga tcttctgcgc gagggccatg tcctcgtcga ccttggccgc
      121 gtacttcttg gcgccgtagg cctccttgaa cttggctttg tcgacgcccg cggcggcggc
      181 ccagttctcg aagttctcat cggtcaggtt ctgctggttg ccgaaagcct gatcgtggaa
      241 cttccagaac gcctggctgc caccgagcgc gaacacggtg ttggccgctt cggccgccgg
      301 acgcgcgcgg ttgtggaagg gcagggggtt gtgcttgaaa acgacgcgga cctggtcctt
      361 gtaggtctct tcgacctgct tgatggtcgg cgtcacgcgc gagcagaacg ggcactgaaa
      421 gtcgctgatc tcgacgatcg tgacgggcgc atcggccttg cccttctgcg ggtctttggc
      481 gctgacgggc acgctggcgg cgctttgatc tgcgaccgcg ccgctgcccg aggcgctctc
      541 ggcctgaatg tcggagccgc cgcgactgcg ctcgaccccc cacatcagga acacgccggc
      601 tatgaagctc atgatgaagc cgacgatggc agttccctta ttcatggaaa actccttcta
      661 aacacacaag atattcgaat tgcggacatt gaaaatcgat gatggagccg cgcgcatcgc
      721 acagctttcg tgtgcgaagt cgatgtgcac gcctccgccg ctcccggttc gaggagctcg
      781 gctttacgcg agttggatgg cggcgaggcg acgtggcggt cgctcgagcg cggaacggtg
      841 agcgctccgc tcgccgtctt gaatcggaac caacggagct cgcgtacgcg gctcgcgcgg
      901 caaatggaag gcgaccggcg ggacgcgtgg gacgacctcg atgctcggga gctcgggagg
      961 cgtctgacgg ggcgcggtcg tctccaagcg cgggtcgctc tgaggatcgc acgcgctcgg
     1021 tccgagcggg atctcttgcg ctgcgcgact gatcggagga gccaccaccg tctcggcgtt
     1081 caccccgcac atcggcacca acgacatggc gtgcgcgcgc ctcgtcccaa agacgagcag
     1141 cgtcagcagg acgacggcaa agagccgcgc cactctgcgg aatcgaatcc gtgccgagtg
     1201 ctgcatgaag gtggccgggc ggagtctagt cgcttgcgac cagggctcaa cgggttgcgt
     1261 tggaggaaca ttcccagggc cgcggaccga cggggcagag ctgaaatgcc tctttcttgc
     1321 ccacagtgtc ccacattgta caccatgcgg gcatgcaggc tctggagctc agcgaaaacg
     1381 ccccgaaatc attggaaaat caggcgtcaa cggcggcacc cgccgaagcg cgcccagaac
     1441 cgcttccgcg ttccttcgaa cgctttaccc ttctctctcg cgtcgcccgc ggaggcatgg
     1501 gggaggtcta cttggcggcc gctcggagca tcgaaggcgc cgagcgcccg ctcatcgtca
     1561 agatcatccg ccccgaccac gccgacgatc gctcgttcgt ggcgcgcttc ctggacgagg
     1621 cgcgcatcca ggctcagctc cagcacccgg gcgtcgctca gatcatcgaa gcaacgacgg
     1681 atcagaccgg taagccctac gtcgtcgtcg agtacgtcga aggtcgcaac ctgagcgacg
     1741 tgcgtgcgcg cgcggggcag ctcggagtgc gcgtgagctg gcccgaggcg ctcgcgctcg
     1801 gcgtgtgcat gggcgaagcg ctcgctcaca tccacgagcg tacggatccc gaaggccgcc
     1861 cgctcgagat cgtgcaccgc gacctcagcc cgcagaacgt gatggtcggc ttcggtggtg
     1921 acctgaagct catcgatttc ggcaccgcgc gcggccagaa ccgtcgctgc caaacgatcg
     1981 ccggcgtcgt gttcgcgaag ccgggctacg tagcgccgga agtcgccaac aattcgcccg
     2041 gaggcatccc cgccgatctc tacgcattcg gggtgatcct gtgggagcta ttggccggac
     2101 gacgttttct cacgggagag gcgacggcgc acatggtcgc ggtcggctcc ggcaagcgca
     2161 cgctgccgcc gctcgctccc atcgtcggtg cgccaccgga gatcgatcag atcctcgcgc
     2221 agcttaccgc gacgaagatc gaagatcgtt acgcgagcgc gcgcgtcgcg acgcaagact
     2281 tggtcaagct ccttcagcgc gctccgagcc tcgccgacgg cgatcgcagc gtgcgtgggc
     2341 gcatctcgga cttgatgcgc cgcctgtatc ctgccgagcc cacccgcagc cgcgccgatt
     2401 ttgctcggcg cgtcgcggag gctcgctcgg tggcgccgcg cgtcgtggcc ccgccgccgt
     2461 ctcccgaacc gccggagagc accgactcgt cggtcctcga tggtacccgc tatcgtctcg
     2521 ggcgactcat tgggcgcggc ggcatgggcg ccgtgcacga ggccgttcac ctcgatctcg
     2581 ggcgcaccgt ggcgctcaag ttgctcgagt cggacacgat gggcgagaag gcgcagagcc
     2641 gcttccgcgc cgaggcgcgc gccatcgccc agctttcgca cgacaacatc gtgcgcgtgt
     2701 acgacttcgg cgcgtgccgc gacggccgtc tgttttacgc catggagctg ctcgacggcg
     2761 agacgctcgc catgcgcctg ctcagcgtgc cgcacttgcc tttcgcgtcc gtcgtcgatt
     2821 tcggcatcca ggcatgccgc gcgctcgaag ccgctcacgc cgcgagcgtg gtgcaccgcg
     2881 acctcaagcc tgcgaatctt ttcgtcacgc agacgggcac tttgaaggtg ctcgacttcg
     2941 gcgtcgcgaa gagcgcggct gcctccgaag ctgcggaaaa ggacgcgatc acgatcgtgg
     3001 gcacgccgga gtacatggcc ccggagcagc tgaaggggac agccgacgcg cgcagtgatt
     3061 tgtatgcgct cggcgtggtg ctcttcgagc tcgcgaccgg cacgctgccg ttcgaagccg
     3121 aaggtccgct cgagatcctc tcgcgcaaga actccgaggt tgctccgcgt gtgcgtgagc
     3181 gaggagccga gcccctgccc cccgcgttcg attcgctgct cgcgcggctg ctcgagcgcg
     3241 acccggcgcg ccgccccgag agcgcccgtg cgcttcgcca ggagctcgag gcattgctca
     3301 cggcgccgcg ctcacgccag actctgcgtc gcaagttcgg cagcgtcgcg gtcggagcgt
     3361 cgctcttcat cggtgcggcc gtgctcgcga acgccggcat gcgcaacacc caagcgagca
     3421 gcgcgctgcg ctcgagcctc ggcgaagtca cggcccgcgt catgagcttc ggtgagcagt
     3481 tgaagcaccg acgtgaggca ctgcttgcag cgcgcactgc gagctctgcg gtcgctcagt
     3541 cccaattagc ggcccagccg gaagcgaaga tcgttgcaaa gcaaccgagc gagcccgctc
     3601 ccgtcacgga ggcgtcaccg aaagagccgg aaattatcga cgcagacgcg ctcgccaacg
     3661 aggtgtcggc ggagcccgtg cgcgcgcccg aagtgtcgag cgagacggcg caggcgctcg
     3721 cggaagcgaa ggccctgacc gacaagggcc gcgagctgaa ggcgctcgac gtgctgcgtc
     3781 gagccgtcga gcgacatcca gacgacgtgg agctgctcgg cgcactttcg caggcgctct
     3841 cgaaaaatcg ctcatggggc gaagcgctgc gcgtcgctcg acaccgggcc gagctcgacc
     3901 agagcgcgcc ggctcgcttc gacctagcgc gcctcgagcg agcgacgggc catcgcgacc
     3961 gagccatcga gctcttgaac ggcttgctcg gggacgatca gcacggcagc gccgcgcggg
     4021 agctcctgaa gagtctgaac ggcgcttctc gcgtcgcgtt gaaggattga gccgcacctg
     4081 tgcccaggcc actcgcccga ggacactccg gccgaggggc ctcgggcttg cggagacgta
     4141 acttgggcgg ctcggcttct cgtatcgccg gatcaattcc gtgcgctata agaggccccc
     4201 aagcctcatg gccctgcccc ctagcgcttc gagcgcgagc gttgcaaccg acggctcccc
     4261 ttatcgggtc gtcctgcggc ttgccacgcc gacgatcctc gcgatgctgg cgcagagcgc
     4321 cgtcaacgag atcgacatca tctttttcgc gcgcctgccg tgccccgagt cgtcgaatgc
     4381 gcaggcggcg ctcttgccgt cgctcatcct gctttggatg ttcggtgggt cgctgagcgc
     4441 gatttcggtc ggcactcagg ctatcgcggc gcgtcgcttc gccgagcgca agcagctcga
     4501 cgcgggcgcc gtgctcgtca actcgtgggt gttctcgctc gtggctggca ttgccttcac
     4561 gctgctcggc tacctcgcga tgccgtcggt gctgaatctg ctcatcaaag tgccggaggt
     4621 tcgcgaagcc gcgcgcacgt acctgggtta tcggttgctc ggcatcacgt cgatggcgat
     4681 gacgttctcg ttcaaagcct tcttcgacgg catcggtcgc acgcacgtcc acatgatcgc
     4741 ctcggtggtc atgaacatcc tcaacgtgct gctgtgcatc gcgttcatct tcggcaactg
     4801 gggagcgccg cgtatgggca tcggcggcgc ggggctcgcc ggcttcgttt cgacgtgggt
     4861 gggtttggcg atcatgatcg cttgggccct gcactcccgg taccggagcc aatacaatcc
     4921 gttctcgctc agccgcgtgt cgaagcgcgg cgtgtgggac atcctaaggc tctcgatccc
     4981 gagcgccatc gcgacgatcg ccgtgatgac ggggttcgcc ctgttctcca cgatcgtgag
     5041 ctacctcgac tcggtttcgc acgcggcggt cctgtcgcct gattgcccgg gcgggaaagc
     5101 tcaggccgta aacagcgccg cgacgatggt gatcgtcggc atcctgaagc ttaccttcac
     5161 cgcctgcctc gcgttcggca cttcgaccgc gacgctcgta agccaatcgc tgggcgaagg
     5221 ctcgcccgaa cgcgcgacgc gcttcggttg ggtctcggtg cggctcggct tgatgatctt
     5281 tggcgtcgtc ggcctcatcg aaggtgtgct gttcacgaac cagattctgg ggttcgtgac
     5341 tcacagcgaa gcggtgctcg ccgcagcctc cggaccgatg cggctcatgg gcatctgcac
     5401 gccgctcatt gccaccggca tgatcctcac gcaggcgttg ttcggcgcgg ggaactctcg
     5461 cttcgtgatg atcgtggagc tggttttgca ctttggctgc ctcgttccgc tcgcctggtt
     5521 gctcggcgtg accctgaact ttggtttgcc ggggatttgg ggggcggcga cggtgtacgt
     5581 ggtgcttctc accgcgatca tggtgatgaa gttccgaagc ggcgactggc aagccattcg
     5641 gctttagcgt cagtgctgaa tcgtggacgg cgatcggctg ccggggcacg ccgaatgggc
     5701 gcgtgtcgca gccagcggtg ctggtcggct tcgctacagt tcggcgccga ttgtcagaat
     5761 tgtcgggttt cggccatggc ttggggctcg attcgggaac gttcagcgtg attgcttcgg
     5821 cgtgagcgtc tccaccccgt ggcgcgcttc ttggaacgcc caagccacga tgcgctcagt
     5881 cgccaaagca acgccagagg cgaccgggag ggcccggtct cacgtggtga agtcggggtg
     5941 agctcggttc gcatccacga actcttggcc cggcgtctca cgacgcatgc gttcgacccc
     6001 gaagagcgcg tttcgagagc cgattgctct gcactcctgc gcgcggctca cgccgcccct
     6061 tcttggaaca accagcagcc ctggcgcttc ctcgtctgcg atcgtttcga gcggcctgat
     6121 gcgtggcagt ccgccttcga cgcgctggag cggaaggagc gaccgtgggc atacaacgcg
     6181 gccctcttca tcgtggtcgc ggtcgacttg gacgac
//
LOCUS       DASTKS010000774         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1686224, whole genome shotgun sequence.
ACCESSION   DASTKS010000774 DASTKS010000000
VERSION     DASTKS010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686224"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2876)
                     /locus_tag="VFQ35_16055"
     CDS             complement(<1..2876)
                     /locus_tag="VFQ35_16055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9932218.1"
                     /translation="MADLLDVVRKACLPAIWSQGVKLARGNAVLADKTSAEERTFRVK
                     AQGLAVAPTVTLYLKEQEWSCDCGGRFDPCAHVAAAVIASEQGLLTVEKDEGATSNGR
                     AAEESAASGGEASAPAAASPKSAARARLEYRLLAKNRGLVLTRVVVHPDGREERITTL
                     ASDIARGRLPREITPTHDDLSIDRILNANRRESLALDRLAVVLRALSTAEHVRLDGKP
                     IKASGELVYPQATLEDGPHGGYRLRLEKDPAVDEVVAMGVARLGDTLRPLGETQSTGY
                     LLERLPLATVFPRERQAELVAHVLPELEAKLKVNVRTTRLPRVAKDVRPRIAFDLSHH
                     GHTLSVLPTLVYGDPPLLRIDGDQAIALSDDVPIRRRDEERARLQELRDGLNLVTGRR
                     IDLDGTEAVRFAAKLRDWQRQSGDVRNAAMFGDKPLVPRLVMNGDEFEVLFETEDGDG
                     DGEATARKRADAASVLRAFRDGLDLVPLDGGGWAPLPAAWLAQHGHRVLDLLAARDKD
                     KKLPTAALPDLGALCDVLNAPKPVSLSKLEPLLKDFTGLPHVELPADLNAELRTYQQF
                     GVDWLSFLRSAGLGAVLADDMGLGKTLQTICVLKGRVLVVCPKSVVHNWAAEIARFRP
                     GLRTTIYHGIRRELEEHADVTLTTYSVLRLDIERLAELDWDIIVLDEAQAIKNAESQT
                     ARAAFQLKGEFRVALSGTPVENRLEELWSLIHFVNPGLLGARSDFQERYATPIAAGNV
                     EAGTRLRSKVRPFVLRRVKRDVLPELPPRTDAVLHVELDENERAVYDAVRAATHKELI
                     ERLSQGGSVMAALEALLRLRQAACHAGLVPGQTAERSSKVDRLLEALDEVSAEGHKAL
                     VFSQWTSLLDRVEPHLKEAGIAFTRLDGSTRDRASVVANFQSESGPPVMLVSLKAGGT
                     GLNLTAADHVFLLDPWWNPAVEAQAIDRAHRIGQACQVFAYRLIA"
     gene            3551..5269
                     /locus_tag="VFQ35_16060"
     CDS             3551..5269
                     /locus_tag="VFQ35_16060"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9932219.1"
                     /translation="MSDSTERAPDANESSKSGAENLPVAEGTRSNDEPWAVYLAKRLV
                     GEKSFQLGVPEEAAGLAEGSDIVLSYGDLSLNLACIVDREGNPEKRFSLEQKELLEIG
                     SACLKYTGTMNGAKMPVGISLYEVGDRPASPEDIERFKALQKSMPGSSKVLVTCFYLD
                     TKNKTVFCSALFNGLFRGRKWLESMLAEPRKRHEDIFVPEALLPSDEGRPVATIGVLV
                     ALASMFVVEQLADAGRQAHGLLGVNVGTLFALGGMNRDAVLTNGEWHRLLSAALLHGD
                     AFHILLNGFALGMAGLVLEPLLGRAWFLSLFFIGALGGSLMGLLVNPANIVSVGASGA
                     VMALLAAAVVVAMRFPRGAQRTQIQVSMLQFLIPSLIPLASHRQQGHIDFAAHFGGAI
                     AGVAAGFALMKIWPRTQERPRFRSAALALAAASLVGFALSLFLTKQHYPEYAAAATFA
                     SKELLVDDDAIPKDFAAAKQQVETWGKDRPRDPRVHLYRAFRLFDEEQLAGAEKELRA
                     ALSEREILERAFSNGKLEASIRSVLCQLLVQQGRTDEARREAQPVCKGSDPKLAEPLK
                     SLGLCE"
BASE COUNT          977 a         1880 c         1997 g         1068 t
ORIGIN      
        1 gcgatcaaac ggtaggcgaa tacctggcac gcctggccga tccggtgcgc acgatcgatc
       61 gcctgcgctt cgaccgccgg gttccaccac ggatccagca gaaatacgtg gtccgcggcg
      121 gtcaggttga gcccagtgcc acccgccttg agtgacacga gcatgaccgg cggtccggac
      181 tcgctctgga aattcgcgac cacgctcgcg cgatcgcgcg tggagccgtc gaggcgggtg
      241 aaggcgattc cagcctcttt caagtgcggc tcgacgcgat cgagcaggct agtccattgc
      301 gagaagacga gcgccttgtg gccttcggcc gacacctcgt cgagcgcctc gagcaggcgg
      361 tcgaccttgg aggagcgctc ggcggtttgc ccggggacca ggccagcgtg gcacgccgcc
      421 tgccgcaatc ggagcaatgc ttcgagcgcg gccatcacgc tgccgccctg gctcaagcgc
      481 tcgatgagct ccttgtgagt ggcggcgcgc acggcgtcgt aaacggcgcg ttcgttctcg
      541 tcgagctcga cgtggagcac ggcgtccgtg cgcggcggga gctcggggag gacgtcgcgt
      601 ttcacgcggc ggagcacgaa gggacgtacc ttggagcgca ggcgagtgcc ggcctcgacg
      661 ttgcccgcgg cgatcggcgt cgcgtagcgc tcctgaaaat cgctgcgagc gccgagcaac
      721 ccagggttca cgaagtgaat caagctccag agctcttcga gccggttctc gaccggagtg
      781 ccgctgagcg cgacgcggaa ctctcccttg agttgaaacg cggcgcgcgc ggtctggctc
      841 tcggcgttct tgatggcctg cgcttcgtcg agcacgatga tgtcccaatc gagctcggcg
      901 aggcgctcga tgtcgaggcg cagcaccgaa taggtggtca gcgtcacgtc ggcgtgctcc
      961 tcgagctcgc gccgtatgcc gtgataaatc gtggtgcgga gcccggggcg gaaccgcgca
     1021 atttccgcgg cccagttgtg caccacgctc ttcgggcaaa cgacgaggac gcggcccttc
     1081 agcacgcaaa tcgtttgcag tgttttaccg agacccatgt cgtcggcgag cacggcgccc
     1141 aagccggcgg agcgcaggaa ggagagccag tcgacgccga actgctgata cgtgcggagc
     1201 tcggcgttca aatcggcggg cagctcgacg tgtggcaggc cggtgaagtc tttgagcagc
     1261 ggctcgagct tggagagtga aaccggcttt ggcgcgttga gcacgtcgca cagcgcgccg
     1321 agatcgggca gggccgcggt cgggagcttt ttgtctttgt cgcgcgcggc gagcaggtcg
     1381 agcacgcgat gaccgtgctg cgcgagccac gccgccggca agggagccca gccgccgccg
     1441 tcgagcggca cgagatcgag cccgtcgcga aatgcgcgca gcacgctcgc cgcgtccgcg
     1501 cgttttcgcg ccgtggcctc gccgtcgccg tcgccgtctt cggtctcgaa caagacctcg
     1561 aactcgtcgc cgttcatgac gaggcgcggc accaagggtt tgtctccgaa catcgccgcg
     1621 tttcgcacgt cgccgctttg tcgctgccaa tcgcggagct tcgcagcgaa acgcaccgcc
     1681 tcggtgccgt cgagatcgat gcgccggccc gtgacgaggt tcaagccgtc gcggagctcc
     1741 tgcagccgcg cgcgctcttc gtcgcggcga cggatcggga cgtcgtcgct cagcgcgatg
     1801 gcttgatctc catcgatgcg caagagcggc gggtcgccat aaacgagcgt tggcagcacc
     1861 gacagcgtgt gcccgtgatg cgacaggtcg aaagcgatgc gggggcgcac gtccttcgct
     1921 actcgcggca gtcgtgtcgt gcggacgttg accttcagct tcgcttcgag ttcgggcagt
     1981 acgtgcgcga cgagctcggc ttggcgctcg cgggggaaca cggtcgcgag cggcaaacgc
     2041 tccaggaggt acccggtgct ctgcgtctcg ccgagcgggc gcagcgtgtc gcccagacgc
     2101 gcgacgccca tcgcgactac ctcgtcgacg gcggggtcct tttcgagccg tagccggtac
     2161 ccgccgtgcg gcccgtcttc gagcgtcgct tgcggataga cgagctcacc ggatgccttg
     2221 atgggcttgc cgtccaggcg cacgtgctcc gccgtcgaca gcgcgcgcaa cacgaccgcg
     2281 aggcggtcga gcgccaacga ctcgcgccgg ttcgcgttca atattcggtc gatgctgagg
     2341 tcgtcgtggg tcggcgtgat ctcacggggt agccttccgc gagcgatgtc cgaggcgagc
     2401 gtcgtaatgc gctcttcgcg cccgtcgggg tgcaccacga cgcgcgtcaa cacgagcccg
     2461 cggttcttgg cgagcagccg gtactcgagc cgagcgcggg ctgcgctctt cggggaagcc
     2521 gccgccggtg ccgaagcctc tcctcccgaa gctgcgctct cctcagcggc tcgtccgttg
     2581 ctcgtcgcac cctcgtcttt ttcgaccgtc agaaggcctt gctcggaggc gatcaccgcc
     2641 gccgcgacgt gggcgcaggg gtcgaagcgc ccgccgcagt cgcatgacca ctcttgctcc
     2701 ttcaagtaca gcgtcaccgt tggtgccacg gcgagccctt gcgctttcac gcggaacgtt
     2761 cgctcttcgg cagaagtctt gtcggcgagc accgcgttgc cgcgcgctag cttcactcct
     2821 tgcgaccaga ttgcgggcaa gcacgccttg cgtacgacat cgagcagatc ggccattcga
     2881 gcgggcggga actttggctc atttttggcc gacgtgcgac ccgcgagcgc agctacgggt
     2941 tgaaaccgtt gcaattcggt ccgaggtcgt tgctcgacgc gcggcttgcg gacgaaacgt
     3001 tgcgacatcg accatctagc cgagtcacgt ggatccgcgg cgttttgctc gagccggact
     3061 tccctgagga ctatcgctcg cgacggtgct acgaacggag gtttgttcct cggggagctc
     3121 gcgcgcggcg taccgtacga cactgcccac tcgctccgcg cttcttcggc ggctggtaac
     3181 cgcggttcgc cctttcggag cggagtggcg ggcgttggct cgtgcacatt tgtccattcg
     3241 aaatgggtga gcggcgaatc gcgagattgg ctccgggagc gatccgcttt gcgcttcggc
     3301 gggcacgacc gttcaggggg gttcttttcg gagcggtgcg ggcgcggtgg cggcttcgcg
     3361 caacgccgag cagtgtctgc gctcgcgacg cccgctccgc cctgcgcgct cttttcggtg
     3421 ctgcgcccaa ccaagaccac gaccgagcag agggagtgcg aaaccggctc ttacgatggc
     3481 agtcgacgcg ttcgatgtcg cccgccgccg aactcgagta cagacgagag aggtgcgagg
     3541 gagagagctg atgtccgact ctacagaacg agcccccgac gcgaacgagt caagcaagtc
     3601 cggtgcggag aatttgccgg tcgcggaggg gacgcgcagc aacgacgagc cgtgggccgt
     3661 ctacttggcg aagcgactcg tcggcgagaa gtcgtttcag ttgggcgttc cggaagaggc
     3721 ggccgggctc gccgagggca gcgatatcgt tctttcgtac ggagacttga gcctcaacct
     3781 cgcttgcatc gtcgatcgcg aaggcaaccc cgaaaagagg ttctctctcg aacagaagga
     3841 gctgctcgag atcggcagtg cgtgcctgaa gtacaccggc acgatgaacg gagccaagat
     3901 gccagtcggc atctcgctct acgaagtcgg cgatcgcccg gcgagcccgg aagacatcga
     3961 gcgcttcaag gcgctgcaga agtcgatgcc gggttcgtcg aaggtgctgg tgacgtgctt
     4021 ttacctcgac accaagaaca agacagtctt ttgctcggcg ctcttcaatg ggctctttcg
     4081 cggccgaaaa tggctcgaaa gcatgctcgc cgagcctcgc aagcgccacg aggacatctt
     4141 cgttcccgaa gcattgctcc cgagcgacga gggacgccca gttgcgacga ttggcgtcct
     4201 cgtcgcgctc gcgtcgatgt tcgtggtgga gcagctcgcc gacgcgggtc gtcaggcgca
     4261 cgggctgctc ggagtgaacg tcggtacttt gttcgcgctc ggggggatga atcgggatgc
     4321 ggtcctcacg aacggtgagt ggcaccggct cctctcggcc gcgctgttgc acggcgacgc
     4381 ctttcacata ttgctcaatg gctttgcgct cggtatggcc ggcctcgtgc tcgagccgct
     4441 gctcggccgt gcttggttct tgagtttgtt cttcatcgga gcgctcggcg gctcgctgat
     4501 gggactactc gtgaatcccg ccaacatcgt gtcggtgggt gcctcgggag cggtgatggc
     4561 gctgctcgcc gcggccgtgg tcgttgcgat gcgttttcca cgcggcgcgc aacggacgca
     4621 aatccaggtg tcgatgttgc agttcttgat tccttcgttg attccgctcg cgagccatcg
     4681 ccagcagggc cacatcgact ttgccgcaca cttcggcgga gccatcgcgg gagtggcagc
     4741 cggcttcgcc ttgatgaaga tctggccgag gactcaagag cggccgcgct ttcgaagcgc
     4801 tgcgctcgcc ctcgcagcgg cgtcgctcgt tgggttcgcg ctcagcctgt tcctcacgaa
     4861 gcagcactac ccggagtacg ccgcggccgc gacctttgcc tcgaaagagc tgctggtgga
     4921 cgacgacgcc atccccaaag acttcgcggc ggccaagcaa caagtagaaa cgtggggtaa
     4981 ggaccgccca cgggacccgc gcgtgcacct ctatcgagca tttcgcctgt tcgacgaaga
     5041 gcagcttgcg ggcgccgaaa aggagctccg cgccgcgctc tcggagcgcg agatcctcga
     5101 gcgcgctttc tccaatggaa agctcgaggc gagcatccgc tcggtgttgt gccaactgct
     5161 cgtgcagcaa ggtcgcaccg acgaggcgcg tcgcgaagcg cagccggtgt gcaaaggatc
     5221 ggatccgaag ctcgcggagc cgctgaagtc actcggtctc tgcgaatagc ctgtcgcgtc
     5281 accacattac tgacatcacg cccggaaata ctcgaggcga cggtaatcgc cgttcgacat
     5341 catcgacgaa cggtgttgcg ccatcgaatg atggagtggt atatgcggtg aatgggtgtc
     5401 gtgaggggga gatccggtcc gcgcgctggt ggtttaccag aatcccgaaa cggaaccttt
     5461 tcagggcttt tcaccagaaa agcactcgca agactagtgg gtggggtagt cgcaattgcc
     5521 agtcttgctg gcgcgtactg gctcggcgcg caaaacgccc caacgcacgt cgctgccgct
     5581 gccgcaccca cgaacgcaat taccaatgcc ccgagtgtgc ccggaatcgt atcgaccgcg
     5641 ggcccaagcc aaccagctcc gcgagaggcg gcgcgtgtta cggtcgctgc ggcggaaccc
     5701 gcgccgggtg agtccctcgc ggcgcccatt gagcctcgcc cgcaactttc cgcgctcgaa
     5761 caccgagatc gagtgctggc tgcactgcgt gcatcgggtc gcgatacccg attgaccagc
     5821 tttgcccaga cggtcgtgaa gggctggtcg agcaagctac agcaattggc ggttggtgga
     5881 aaagtcgagc cgctcgaatg ctacgcgaag gggtgcgcgg tg
//
LOCUS       DASTKS010000775         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_124393, whole genome shotgun sequence.
ACCESSION   DASTKS010000775 DASTKS010000000
VERSION     DASTKS010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124393"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFQ35_16065"
     CDS             complement(<1..1049)
                     /locus_tag="VFQ35_16065"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9932220.1"
                     /translation="MKQFLRLASSCLLALACAPKPVAAPAPQAPPPAPVEQEVAPKPS
                     DTIEPPTSGISPSAPFPTIVHRDLENGLGLRVVERHVHPIVELRLVVRSGSASDGEKP
                     GLAIVAGELLKAGGAGGMTPEQLIRRAESLGTNLDITTDRDSTRIALGVPSGDFERAV
                     DILAAVAQKPAFAPVEFNKLRTREIERVKSSARGSATWAAAMVLYRELFEIPTAVHPY
                     SRFDAQPAELEKLTLADCKQWHRTHFVPANASLVVVGDVTPDRVEAAAKKAFGEWKSQ
                     PAPTLSVSAPFPPAQRDVYLVDRPGAAQSQIYVGMLGLSRTDPDYPALAAANQVLGGG
                     VSGRLFLDVREKRSLA"
     gene            complement(1046..>2028)
                     /locus_tag="VFQ35_16070"
     CDS             complement(1046..>2028)
                     /locus_tag="VFQ35_16070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9932221.1"
                     /translation="LFLEADRMRWLAVNAKNFENQRAVVQEEYRMRVENAPYAKGLNR
                     LEEITFADYGPYAHTTIGSMESLAVAKLEWVTEFYKTYYAPNNAVLTIAGDFDSDRAM
                     ELVRKYFGAAERRSVPAFDASAPAPRPDAKLTRREVVEDKNVNTPGVLWAFRIPPARS
                     ADHYALELASQLLSDGESSRLHQKYVRDRPILQRVGSWTYDRRGPDELAIMGILTEKA
                     KPEEVERAFTAELEALRKTPPSLEELTRIKARLKHDFLFELQSNSRRAIRLGEYEVFF
                     GDARILTRELAAYLAVTPEDIRRVANEYLKPELRFAVDVRPAARSAGGAK"
BASE COUNT          313 a          642 c          709 g          364 t
ORIGIN      
        1 gcgagcgagc gcttctcgcg cacgtcgagg aagaggcgac cggaaacgcc gccgccgagg
       61 acctggttcg ccgcggccaa cgccggataa tccgggtcgg tgcgcgagag gccgagcatc
      121 cctacgtaga tttggctctg cgcagcgccc ggacggtcca cgaggtagac gtcccgctgc
      181 gctggtggga acggggcgct cacgctgagc gtcggcgccg gctggctctt ccactctccg
      241 aacgcctttt tcgcggcggc ttcgacgcga tctggcgtga cgtctccgac gacgacgagg
      301 ctcgcgttgg cggggacgaa atgcgttcgg tgccactgct tacaatcggc gagcgtgagc
      361 ttctcgagct cggccggctg cgcatcgaag cgggagtaag ggtgaaccgc cgtcggaatc
      421 tcgaacaact ctcgatacag aaccatcgcc gcggcccagg tggcgctgcc gcgcgccgaa
      481 cttttcacgc gctcgatttc gcgcgtgcgg agcttgttga actcgaccgg cgcgaaggca
      541 ggcttttgcg cgacggccgc gaggatgtcg acggcgcgct cgaagtcgcc gctcggtacc
      601 ccgagcgcga tgcgcgtcga gtcgcggtcg gtcgtgatgt cgaggttcgt ccccagggac
      661 tccgcgcgac ggatgagctg ctcgggcgtc atccctccgg cgccgccggc cttcaagagc
      721 tcgcccgcca cgatcgcgag tccgggcttc tcgccgtcgc tcgcgctgcc cgagcgcacg
      781 acgaggcgga gctcgacgat gggatgaacg tgacgctcca ccacgcgcag cccgaggccg
      841 ttttcgagat ctcgatgcac gatcgtgggg aagggggcgc tcggcgagat ccccgacgtt
      901 ggcggctcga tcgtgtcgct tggcttcggc gcgacttctt gctcgaccgg cgcggggggc
      961 ggcgcttgcg gcgcgggcgc agcgacaggc tttggggcgc aagcgagcgc gagcagacac
     1021 gacgaggcga gacgtagaaa ctgtttcact tggcacctcc ggcgctgcgg gcggcgggac
     1081 gcacgtccac cgcgaagcgc agctcgggtt tcagatattc gttcgcgacc cgacggatgt
     1141 cttcgggggt caccgcgaga tacgccgcga gctctcgcgt gaggatgcgg gcgtcgccga
     1201 agaatacttc gtattcaccg aggcgaatcg ctcggcgcga gttcgattgg agctcgaaca
     1261 agaagtcgtg cttcaagcgg gccttgattc gggtcagctc ctctaacgaa ggtggagtct
     1321 tgcgcagcgc ctcgagctcg gcggtgaacg cgcgctcgac ctcctcgggc ttggccttct
     1381 cggtgaggat ccccatgatc gcgagttcat cagggccgcg gcggtcgtag gtccagctcc
     1441 ccacgcgctg tagtatcggc cggtctcgca cgtacttttg gtgcagccga gagctctcgc
     1501 cgtcgctcaa gagctggctc gcgagctcga gcgcatagtg atcggcgctg cgggcagggg
     1561 ggatacggaa ggcccagagc acgccgggcg tgttcacgtt cttgtcttcg acgacctctc
     1621 gtcgggtgag cttcgcgtcg ggccggggcg cgggcgctga cgcatcgaag gcagggacgc
     1681 tacggcgctc ggcggccccg aagtacttgc gaacgagttc catcgcgcgg tccgagtcga
     1741 agtcgccggc gatggtaagc accgcgttgt tgggcgcgta gtacgttttg tagaactcgg
     1801 tcacccactc gagcttcgct accgcgagac tctccatgct gccgatggtg gtgtgagcgt
     1861 agggcccgta gtccgcgaac gtgatctcct cgaggcggtt caagcccttc gcgtacggcg
     1921 cgttctcgac gcgcattcgg tactcttctt gaacgaccgc ccgctggttc tcgaaattct
     1981 tcgcgttgac ggcgagccac cgcatgcgat ctgcttcgag aaacaagg
//
LOCUS       DASTKS010000776         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_346692, whole genome shotgun sequence.
ACCESSION   DASTKS010000776 DASTKS010000000
VERSION     DASTKS010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346692"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            529..2184
                     /locus_tag="VFQ35_16075"
     CDS             529..2184
                     /locus_tag="VFQ35_16075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9932222.1"
                     /translation="MSKRVLIVGGVAGGASAAARLRRLDESSEIVLFDRGPYVSFANC
                     GLPYHVGNVIEEERKLLLASSSLFRQRFNIDVRTEHEVLAIDRANSTLRVRDLKTGLE
                     RDERWDSLVLAPGAAPLIPPIEGRDLNGVFVVRTIPDTRRIRAFLEQQRRKTAVVVGG
                     GFIGLEMAENLVHRGLSVTLIEKLPQILPPLDPEMAESVASHLRDAGVKLCAAEGLTR
                     IAQDGERLRVETDRGQAHPADLVILALGVRPETALARDAGLAIGERGGIVVDEQQQTT
                     DARILAVGDAVEVRDSVSGQEVILPLAGPANRQGRVAAETIARGTARFRGVQATAIVG
                     LFGLSAATTGANEKGLRRLGRNDYHVIYLHPGHHAGYYPGAKPVHMKLIFDQQGKILG
                     AQAVGSEGVDKRIDVIATVMKLGGSVYDLAELELCYAPQFGSAKDPSNVAGMMAVNVL
                     RGDMVLADWSSVTDPSHLLLDVREPNEYASGHIEGATNIPLSELRQRAPELPRDRPIF
                     VYCAVGQRGYFAQRFLSQNGYDARNLSGGFTTWKAFCGSQRKL"
     gene            2267..3700
                     /locus_tag="VFQ35_16080"
     CDS             2267..3700
                     /locus_tag="VFQ35_16080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HET9932223.1"
                     /translation="MSVLTTLRHYALAFSGPFAVLAAIAGTVAAAHREERRDQAPGFR
                     ERPRAHFDHTPVIPAHFASPQAVTRKCLECHPDAAAVMKTSHWLWLGPESKIAGREGK
                     QRIGKKNVLNNFCISTRGNERACTKCHAGYGWEDASFDFSKTENVDCLVCHEHSGVYV
                     KGTAGLPTKDSDLAIAARSVGMPSRENCLGCHAYGGGGQAVKHGDLDSSLLHPFADQD
                     VHLGKHRLDCIDCHRAPKHQLRGRAFSVSAEDSNGVRCNDCHANVRHRDTRIEAHLAS
                     LACQSCHVPNFARTVPTKATWDWSQAGDGQRRDDPHHYLKIKGEFTYEQDVVPEYRWF
                     NLTVARYLAADPIAKAGVTTLNAPQGDVRDRSARIWPFKVHRARQPYDAVNGYLLQPV
                     TGGEGGYWTTFDWNSAFELGARATGVPYSGQFGFADTEMYWPITHMVAPKEQALGCTS
                     CHGPAARRMDWMALGYAGDPIKTGARR"
BASE COUNT          694 a         1203 c         1201 g          631 t
ORIGIN      
        1 tgcttcgttt gtgtgggttt cggggcccca gccccaaaac cggcgggctg acgactagtt
       61 tcgagttctg aaccctgaca gcgcgcccgc aggatgcgca ctccctccga cctcactcgt
      121 cgctcggtcg gcgggctgtg gaattttacg ggttcgaggg gctcgcgccg actccttttg
      181 ggtcgcggtt ggctccaacg aggcgaagga atcatgaacg aaaccggaac ctcccgtggc
      241 tcgaacttcg ccactgacgg ggatcatggc ccttcgttca tcatcggccg aactacaagt
      301 gggccgtggc gcgagcgcgc tgaaagagag cgatcatgtg tcgacgagtg acctgtgaaa
      361 tctgcggaaa accaagcttt gcgggctgcg gcgcccacgt cgagcaggtt ttgtcggacg
      421 ttccgaagag cgagcgctgc cgctgccggg aagaacctcg aaagaagtcg gacaccaaag
      481 aggagaggcc ctccttttgg aagcgcttga ccgcgcgcta ggacccccat gtcgaagcgc
      541 gtcctcatcg tcggcggtgt agcgggcgga gcctctgcgg cggcgcgact acgcaggctg
      601 gacgaatcga gcgaaatcgt tttgttcgat cgcggcccct acgtctcgtt cgcaaactgc
      661 gggctgccct atcacgttgg aaacgtgatt gaagaggagc gcaagctact cctggcttcg
      721 agctctctgt ttcgacagcg cttcaacatc gacgtaagga ccgagcacga ggtcttggcg
      781 atagatcgcg ccaattcgac cctacgcgtg cgcgacctca agactggcct ggaacgcgac
      841 gagcgctggg acagcctggt gctggccccc ggcgcggccc cgttgattcc accgatcgaa
      901 ggccgcgacc tgaacggcgt cttcgtcgtt cgcaccatcc ccgatacgcg acgcatccgg
      961 gcctttctcg agcagcagcg ccgaaagacc gccgtcgtgg tgggaggcgg gttcatcggc
     1021 ctcgaaatgg ccgagaatct ggtgcaccgg ggcctctccg taaccttgat cgagaagctg
     1081 ccgcagatcc ttccaccgct cgaccccgag atggcggaga gcgtggcgtc gcacctgcgc
     1141 gacgccggcg tcaagctgtg cgcagcagag ggactcacgc gcatcgcaca agacggcgag
     1201 cgactgcggg tcgaaacgga tcgcggacaa gctcaccccg ccgacctcgt gatcctggcg
     1261 ctgggagtgc gccccgagac cgcgctcgcg cgcgatgcgg ggctcgcgat cggggagcgc
     1321 ggtggcatcg tcgtcgacga gcagcagcaa acgaccgatg cgcgcatcct cgcggtaggc
     1381 gacgcggtcg aggtgcgcga tagcgtgagc gggcaggagg tgattctgcc gctcgccggc
     1441 cccgccaatc gccagggacg cgtcgcggcc gagaccatcg cgcgcggaac cgcgcgcttt
     1501 cgcggcgtgc aagcgaccgc gatcgtgggc ctgttcggct tgagcgctgc gaccaccggc
     1561 gccaacgaga agggccttcg acgcctcggt cgcaacgact accacgtgat ctacctgcat
     1621 ccaggtcatc acgccgggta ctatccaggc gcgaagcccg tgcacatgaa gttgatcttc
     1681 gaccagcaag ggaagatctt gggcgcgcag gcggtcggga gcgaaggcgt cgacaaacgc
     1741 atcgacgtca tcgcgaccgt catgaagctg ggcggctccg tgtacgatct cgccgagctc
     1801 gagctctgtt acgcgccgca gttcggcagc gccaaagacc catcgaacgt cgccggcatg
     1861 atggccgtga acgtcttgcg tggcgacatg gtgctcgccg attggagttc ggttacggat
     1921 ccatcacacc tattgctcga cgttcgcgag ccgaacgaat acgcgtcggg ccacatcgaa
     1981 ggcgcgacga acatcccgct ctcggagctc cgccagcgcg ctccggagct gccgcgtgat
     2041 cgaccgattt tcgtgtactg cgcggtgggt caacgcggct acttcgcaca gcggttcttg
     2101 tctcaaaacg gttacgacgc gcgaaacctc tctggcggtt tcacgacctg gaaagcgttc
     2161 tgcgggtcgc aacgaaagct ctaggaagaa ctcggagcgt cccattcggc gtcggcgcgc
     2221 gtttcgagcg ccgtcgttcg cgcgtgccct tgcccaagag ttcgtcatgt ccgttctcac
     2281 tacgctcagg cactacgccc tggcattctc gggccctttc gcggtactcg cggcaatcgc
     2341 cggcaccgtc gcggccgcgc atcgggagga gcgtcgcgac caagctccgg ggttccggga
     2401 gcgcccgcgt gcgcacttcg atcacactcc ggtgatcccc gcgcactttg cgtcaccaca
     2461 ggcggtgacg cgcaagtgcc tcgagtgtca ccctgacgcg gccgcggtaa tgaagacctc
     2521 gcactggctt tggctcggtc ccgagtcgaa aatcgcaggg cgcgaaggca agcagcgtat
     2581 cggcaagaag aacgtactga acaacttctg catctcgacg cgcggcaacg agcgcgcgtg
     2641 caccaagtgc cacgcgggtt acggctggga ggacgcgagc ttcgatttct ccaagaccga
     2701 gaacgtggac tgcctggtgt gccacgagca ctcgggagtg tacgtcaagg gaactgccgg
     2761 cttgcccacg aaggatagcg acctcgccat cgcggcgcgc tcggtgggca tgccctcgcg
     2821 cgaaaattgc cttggctgcc atgcgtacgg cggcggcgga caagccgtaa aacacggtga
     2881 tctcgattct tcgttgcttc acccgttcgc cgaccaagac gttcacttgg gtaagcaccg
     2941 cctcgactgc atcgattgtc atcgggctcc caagcatcaa cttcgaggtc gcgccttctc
     3001 ggtgagcgcc gaagacagca acggagtcag gtgcaacgac tgtcacgcca acgtacgcca
     3061 tcgagacacg cgcatcgagg cacacctcgc gagcctcgcg tgccagagct gccacgtgcc
     3121 gaacttcgcg cgcaccgttc ccaccaaagc gacgtgggac tggagccaag ccggtgacgg
     3181 ccagcgacgc gacgacccgc accactacct gaaaatcaag ggcgagttca cgtacgagca
     3241 agacgtcgtg cccgagtatc gctggttcaa cttgaccgtc gcgcgatacc tcgccgccga
     3301 tccaatcgcg aaagccggcg tcaccacgct gaacgcgccc cagggtgacg tgcgcgaccg
     3361 cagcgcgcgc atttggcctt tcaaggtgca tcgagcgcgt caaccctacg acgcggtgaa
     3421 tggctacttg ttgcaaccgg ttacgggcgg agagggtggg tattggacta ccttcgactg
     3481 gaacagcgcg ttcgagctgg gggctcgcgc gaccggcgta ccttacagcg ggcaattcgg
     3541 gttcgccgac accgagatgt actggcccat cacgcacatg gtcgcgccca aagagcaggc
     3601 gctcgggtgc acgagctgtc acggtcccgc agcgcggcgc atggattgga tggcgctcgg
     3661 gtacgcaggc gaccccatca agacgggagc ccggagatga agccagtctt tcgcctccgc
     3721 gaggccgcg
//
LOCUS       DASTKS010000777         6631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1240459, whole genome shotgun sequence.
ACCESSION   DASTKS010000777 DASTKS010000000
VERSION     DASTKS010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240459"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1402
                     /locus_tag="VFQ35_16085"
     CDS             446..1402
                     /locus_tag="VFQ35_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HET9932224.1"
                     /translation="MQSLSLLNPKLDLVFKILFGRIENRELLISLVSAVLNPPIPISS
                     VDVLDPEMPRGGVDERGIVLDLRVRLDDGRQVNVEMQAQLRPAQRERAVYHFARMYGS
                     QLVRGEDYRDLRGCVVIFLCDFAADASDRFHSVFRFTEIHDRSTLSEQAEIHFVELPR
                     LAQDPTAKHERFLSAWAKFLSASDEHDFDELSKVDPIFRKATMALKALSEDAKLRLLA
                     ERREAELLFQEYERNVARREAQEEGRREGLEEGRREGLQEGRREGLQQGMTDLLLKLL
                     AQKFGEVPNTVRARVEHASESDLVEWARRVLVEPTLTAVFRE"
     gene            complement(1651..2622)
                     /locus_tag="VFQ35_16090"
     CDS             complement(1651..2622)
                     /locus_tag="VFQ35_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932225.1"
                     /translation="MTTTLLGVDDSKTMRKVIEITFAGEDFKTVLAENADDAMSKLSA
                     DRPNIVLVDAALEGTNGYDLCQRIKAASPGVGVVLLASKQQPYDRGRGSQVGADDFVD
                     KPFDTQQLIDKVAALARKLAGGTQSHPMATTQHGIGPAAAPAAAPFTAPAAHAPQAVV
                     SARPVSLDVGTRPRSPTLAYGNVNPMAPAGPGPVAPMIPSPAAPAAPRSNPIAAPAPA
                     PAAARPAAPAYSPPSPAAYSAPTPPPPAPPAAAPVAAAAPIAAAAAAVTNGADFATKL
                     GSLGLTKDQIAGVLALSREVVEKVVWEVVPTLAETLIKEEIKRLTAE"
     gene            complement(2710..3513)
                     /locus_tag="VFQ35_16095"
     CDS             complement(2710..3513)
                     /locus_tag="VFQ35_16095"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET9932226.1"
                     /translation="MTTPKAAPKSKANPAPRTKPKTQTAAKQRGKTPHKQASGERSKG
                     KRKSKRAFRRHREASPPVGPADSPAIRYGALEPADCLGELRARGIAFREEPTTRGVSI
                     PVRLLGPLAGVSYRTDLSDQQRMNTPWEIYDCRLVLSLADFSEIIAKHGIREVRIFSA
                     YRPPPKNGGDQGSKRHQAALAVDVRTLKKESGEELVVLDHFEPHPGVEPCSEAREPSD
                     PAAKELRAITCETARAHLFNSILTPNFDDRHRNHFHLEVAPGKAWFLLR"
     gene            3676..4695
                     /gene="hrcA"
                     /locus_tag="VFQ35_16100"
     CDS             3676..4695
                     /gene="hrcA"
                     /locus_tag="VFQ35_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET9932227.1"
                     /translation="MLYALVTEFIATGEPIGSRTLSKKYGFLLSPATIRNVLADLEES
                     GYLAQPHASAGRIPTATAFRFFIDALMQERKLPEGEANRISELAELPPGVDLLRETGK
                     LLSDMSGATAVLVRMRTDRRVLSKVHFIRTRPQELLAVLVATDGTVDNRFIALDGPID
                     DAAIERLHNLLDEVVVGRTLSALRDHFELAALDQHGELVKLMQLGHSLLEQIASNSDR
                     RADVVVEGQARLLDQREFGNVEVIRELLVALEDRERLVMLLDQAINADRVNVFLGEDT
                     RRMVGCAVSLVAAGYRDEDGAPGGALGIIGPQRMDYPFVVPLVGATADAMSVALAKKR
                     DADRS"
     gene            4739..6076
                     /locus_tag="VFQ35_16105"
     CDS             4739..6076
                     /locus_tag="VFQ35_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9932228.1"
                     /translation="MARPSTPDLLRPIDHALSRIAEDDPEGSLRYAIPLAQREPTGAL
                     EAFVVGYALSLLSEPELAGKALERAALAAVTESNLPLALTAALVSKRVDAKASQALLS
                     RIARAFGRGSSLLGERRLAPPNLPGASESFKTIDDGVERKDLVARARKLLDAAADIGE
                     PAPRSLAPQPLFSSLAADDLALFAATFKVRVYGAGAVVVEEGTVGSEAFIIARGELDV
                     EKGALQPGLVAVHLARLGTGALVGEMALLLRSPRAATVTAARPSIVLVADKEALDGVA
                     ERAPVVAQRFAEHCKRRMLDNLVRTSPLFHAATPAERPALVERFGIRTFEPGEKLARQ
                     GAPSDGLLLIASGEVSIVHKEGDDRTLVTRLGPGEVVGEVALVLRRPAIADAVAQSPT
                     VCLFLPKERFLELVRSHPKVFADLYEIAVRRDAEIESISQEEAAVEGDDFVLV"
     gene            complement(6391..>6631)
                     /locus_tag="VFQ35_16110"
     CDS             complement(6391..>6631)
                     /locus_tag="VFQ35_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932229.1"
                     /translation="EGIAKSCYILSNDLDFLADAGGGYILPHGTSYVTYYGSGLNGIY
                     ESGVARTAACTNLAQGGDVHPGVQKHCWGRSDLLQ"
BASE COUNT         1125 a         2097 c         2227 g         1182 t
ORIGIN      
        1 ccggaggcgg aggcattgtg tattgtgtgg cgacttgcga cgttttgtgc gcaacgaact
       61 cagtgctcag cgcttggaac cgcgccatga gtttcatgcc gcgcgacctc cgatccgatt
      121 gcggaagttt cgctccttgc cccgcgctcg ccgtgcccgg gagccggtca gggtagaact
      181 gtctttctcg acgactcggc acgacggagc cttccgcccg aacgcgctac gtcgaagcag
      241 gaccgtttcg cgggtctctg caggagcttt tctgcgcttc ccagggcctc gaactccatt
      301 gcatgaccgg agccgcgcgc ccgaccttgc tacgctccag gagcacgggt tcgaaccctc
      361 cggcatattc tgtttgcaag cgttgctcca agcttcggtc caaaattgga gcaactcgct
      421 tcgcgccgcg accgataggg gctgaatgca atcgctctcg ctcctcaatc cgaagcttga
      481 tctcgtcttc aagatactgt ttggccgaat cgaaaaccgc gagcttctca tttcgctcgt
      541 gagcgcggtc ctcaatcctc caatcccaat cagctccgtc gacgtactcg atcctgaaat
      601 gccgcgaggc ggcgtcgacg aaagaggaat cgtcttggac ctccgagtgc gcctcgacga
      661 cggccgccaa gtgaacgttg aaatgcaagc ccaactgagg cccgcccaac gcgagcgtgc
      721 cgtatatcac ttcgcacgga tgtacggcag ccagctggtc cgcggtgagg actaccggga
      781 ccttcgcggc tgcgtcgtca tcttcctctg cgatttcgct gccgacgcaa gcgatcggtt
      841 ccattcggtc tttcgcttca cggaaatcca cgatcgctcg accttgtcgg agcaggccga
      901 gatccatttc gtcgaattgc cccggctcgc gcaggaccct acggcaaagc acgagcgctt
      961 cttgtcagct tgggctaaat tcctgtccgc ctcggatgag cacgatttcg atgagctctc
     1021 caaggtcgat cccatctttc ggaaggctac catggcactg aaggcgctca gcgaagacgc
     1081 gaagctccgc ctgctcgccg agcggcgtga ggcagagctc ctctttcagg agtacgagcg
     1141 aaacgtcgca cgacgcgaag ctcaggaaga aggtcgtcgc gaaggactgg aagaaggtcg
     1201 tcgcgaggga ctgcaagaag gtcgccgcga gggactgcaa cagggcatga ccgacctgtt
     1261 gctcaaactg ctcgctcaaa agtttggcga ggtgcccaac accgtccgcg cgcgcgtcga
     1321 acacgccagc gaatccgacc tcgtcgaatg ggcgcgccgt gtgttggtgg aaccgacact
     1381 caccgccgtg tttcgcgagt aagtcgagca acgggggact cgtgtgtccc cgcgaagtac
     1441 tcgggccggc acccgctggg ccatgaatgg tcgacgagcg cccgagctaa cggcaggacg
     1501 cgcccggaag ttcgcacggc gcccgaacga agagagcgcg cgaagagagc gaacgaagag
     1561 agcgcgcgaa gcgaagcgcc cgcaacggtt aggcgagcgg ggatgtgcca tcgcccgcga
     1621 ttagcggtgg caccggacgc gacccaagcg tcactccgcg gtgaggcgct tgatctcttc
     1681 tttgatgaga gtctcggcga gggtcggaac tacttcccac acgaccttct cgacgacttc
     1741 gcgcgagagg gcgagcacgc ctgcgatttg gtccttcgta agaccgagcg agccgagctt
     1801 cgtcgcgaag tcggcgccgt tcgtgacggc cgcagctgct gccgcgatgg gtgcagcggc
     1861 agcgaccggc gccgccgcgg gaggcgcggg gggcggcgga gtcggcgcgg agtacgccgc
     1921 gggcgacggc ggcgagtagg caggagcagc cggacgggcc gcggcaggcg caggcgccgg
     1981 tgcagcgatt ggattcgatc gcggagcggc aggtgcggcc ggcgaaggaa tcatcggggc
     2041 aacggggccg ggccccgcgg gagccatcgg gttcacgttg ccgtaggcga gcgtcggcga
     2101 gcgggggcgc gttccgacat cgagcgaaac aggtcgcgcg ctgacgaccg cttggggcgc
     2161 gtgggcagcg ggcgccgtaa acggagccgc tgcgggtgcg gcagcaggcc cgatgccgtg
     2221 ctgggtggtc gccatcggat gcgactgcgt gccgccggca agctttcgcg cgagcgctgc
     2281 gactttgtcg atgagttgct gcgtgtcgaa gggcttgtcg acgaagtcgt ccgccccgac
     2341 ctgcgagccg cggccgcgat cgtacggctg ctgcttgctc gcgaggagca cgacgccgac
     2401 gcccggcgaa gcagctttga ttcgctggca gagatcgtaa ccgttcgtgc cttccaaagc
     2461 ggcatcgacg aggacgatgt tgggtcggtc cgcgctgagc ttggacatcg catcgtccgc
     2521 gttttcagcg agcactgtct tgaaatcttc gcccgcgaag gtgatttcaa tcaccttgcg
     2581 catggtcttg ctgtcgtcga cgcccagcag cgtggtggtc acggatcctc caaattaaaa
     2641 tcggacggcg cgaggatcat cgagggcccg gcacaccgtg tcaaggtttt cgcccgaaaa
     2701 gctcggcact taacgaagca ggaaccaagc cttgccaggt gctacctcga gatgaaagtg
     2761 gtttcgatgt cggtcgtcga agttcggggt cagaatgctg ttgaagaggt gggcgcgcgc
     2821 ggtttcgcac gtgatcgctc gaagctcttt ggccgcggga tctgacggct cacgggcctc
     2881 cgagcagggc tcgacgccag ggtgcggctc gaaatggtcg agcacgacga gctcttcgcc
     2941 cgactctttt ttgagtgtgc gcacatccac cgcaagcgcc gcctgatgtc gtttgctgcc
     3001 ttggtccccg ccatttttgg gagggggacg ataggcagag aagatgcgca cctcgcggat
     3061 gccgtgcttg gcgatgattt cgctgaaatc agcgaggctc agcacgagcc ggcaatcgta
     3121 gatctcccaa ggcgtgttca tgcgttgctg gtcggagaga tctgttcggt aagacacacc
     3181 agcgagcggg ccgagcagcc gcaccggaat gctgacgccg cgcgtagtgg gttcctcgcg
     3241 gaaggcgatg ccgcgagcgc ggagctcacc cagacagtcg gcgggttcga gcgcgccata
     3301 gcggatggcg ggcgagtcgg cgggcccaac cggcggcgag gcctctcgat gacgacggaa
     3361 tgcccgtttg gacttccgtt tccctttgct ccgctcgccc gacgcttgtt tgtgcggtgt
     3421 cttgccgcgt tgtttggcgg cggtctgggt ttttggcttc gttcttggcg ccgggttcgc
     3481 cttcgacttg ggagccgctt tgggggttgt caccgagtcc ccacggggcg cggccgcggt
     3541 ttcggcgcga gcggtcgccg taacgagtgc agaaatcgct agaaccaacg cgccagccac
     3601 gctgcgattt gacgagcacc tggatttggg gccaaatttc aagtcgtgaa ccctcttccg
     3661 cgacgtgccc gctctgtgtt gtacgcgctg gtgaccgagt tcattgcgac aggggagccg
     3721 attggcagcc gaaccctgag caagaagtac ggctttttgc tctctccggc caccatccgc
     3781 aacgttctgg ccgatctaga ggagtcgggc tacctcgctc aacctcacgc ctccgcgggg
     3841 cgaattccga cggcgacggc gttccgcttc ttcatcgacg cgctgatgca ggagcggaag
     3901 ctgcccgaag gggaagccaa ccgcatcagt gagctggccg aattgccgcc tggcgtggat
     3961 ctgctgcgcg agaccggcaa gctgctttcg gacatgagcg gggcgacggc ggtgctggtg
     4021 cggatgcgca cggaccgccg cgtcctgagt aaggtgcact tcattcgaac acgcccgcaa
     4081 gagctcctcg cggtgctcgt ggcgacggac ggcaccgtcg acaatcgctt catcgcactc
     4141 gacggtccga tcgacgacgc ggccatcgag cgcctgcaca acttgttgga cgaggtggtc
     4201 gtgggtcgca cgctgtccgc gctgcgcgat cacttcgagc tcgcggcgct ggatcagcac
     4261 ggcgagctcg tgaagttgat gcagctcggc cacagcttgc tcgagcaaat cgcgtcgaac
     4321 tcggatcgcc gcgccgatgt cgtggtcgaa gggcaagcgc gtctgctcga tcagcgcgag
     4381 ttcggcaacg tcgaggtcat tcgcgagctg ctcgtcgcgc tcgaagatcg cgagcggctc
     4441 gtcatgctgc tcgatcaggc catcaacgcc gatcgggtca acgtgttctt gggggaggac
     4501 acgcggcgaa tggtgggctg cgcggtgagc ctcgtcgccg ccgggtaccg cgacgaagac
     4561 ggagcgccgg gcggagccct cggcatcatc gggccccagc gcatggacta cccgttcgtc
     4621 gtcccgctcg tcggcgcgac cgcggacgcg atgtcggtcg cgctggcgaa gaagcgcgac
     4681 gcggaccgct cctgattccg aacgccagat tcgaaacgac cgtgatacgc tcctttcgat
     4741 ggccaggccc tcgactcccg acctgctccg tcccatcgat cacgcgcttt cgcgcatcgc
     4801 ggaggacgac cccgaaggtt cgttaaggta cgcgattccg ctcgcccagc gcgaacccac
     4861 cggggcgctc gaggcgttcg tggtcggcta cgcgctttcg ttgctctccg agccggagct
     4921 cgcggggaag gcactcgagc gcgcggccct cgcggcggtc accgagagca atctaccgct
     4981 cgcgctcacg gccgcgctcg tgagtaagcg cgtcgacgcc aaagcgtcgc aggcgctgtt
     5041 gtcacgcatc gctcgcgcgt tcgggcgcgg gtcgagcttg ctcggtgagc gacgcctcgc
     5101 accgccgaac ctgcccggcg caagcgagag cttcaaaacc atcgacgacg gcgtcgagcg
     5161 aaaagacttg gtcgcgcgcg ctcgcaaatt gctcgacgcg gccgcggaca tcggcgagcc
     5221 ggcaccgcgc tcgctcgctc cgcaaccctt gttctcgtcg ctcgcggccg acgatctcgc
     5281 gctgttcgct gccacgttca aggtgcgcgt gtacggcgct ggcgcggtcg tggtcgaaga
     5341 gggcacggtg ggctccgagg cgttcatcat cgcgcgaggc gagctcgacg tcgaaaaggg
     5401 agcgctgcag ccagggctcg tcgccgtgca cttggctcgg ctcggcacgg gggcgctcgt
     5461 cggtgagatg gcgctcctct tgcgctcgcc gcgcgcggca acggtcacgg cggcgcgacc
     5521 cagcatcgtg ctcgtcgccg acaaggaagc gctcgacggt gtcgccgagc gcgcgcccgt
     5581 cgtcgcgcag cgctttgccg agcattgcaa gcggcgcatg ctcgacaact tggtgcgcac
     5641 gagcccgctg tttcacgccg cgactccggc ggagcgcccg gccctcgtcg aacgcttcgg
     5701 catccgcacc ttcgagcctg gagaaaagct cgcgcgtcag ggcgcgccga gcgacggctt
     5761 gctgttaatt gcctcgggcg aagtctccat cgttcataag gagggcgacg accgcacgct
     5821 ggtcacgcgc ctcggtcccg gtgaggtggt gggggaggtc gcgttggtgc tgcgccgtcc
     5881 cgccatcgcc gacgcggtcg cgcagagccc gacggtgtgc ctcttcttgc cgaaagagcg
     5941 cttcctcgag ctcgtccgct cgcaccccaa ggtcttcgcg gatctctacg aaatcgccgt
     6001 tcgccgtgac gccgagatcg agagcatcag ccaggaagaa gccgccgttg agggcgacga
     6061 cttcgtgttg gtctgattcg agcggaaagg ggaagcggcc gcgctcgggg cggtgcgaca
     6121 ggcggcaact tttcggcgtt accacctcca aaatcaaatt acagctcacc ccgcctcgaa
     6181 tggaaccgcg ccgacgttga gagtttcgaa gttcttctgg cgatccgccg agcgtcgcta
     6241 acgtgagttg cgcgctgccg acagggataa ggtggcagcg cgccgtgcgt cccgaaacgg
     6301 gctcacgacc gcgctagaac ggctcggcgc atgcgacacg aacacgaacg gcgccgcgtg
     6361 gctcgaagcc gccgctcttc ttgacgcgcg tcattggagg aggtccgacc tgccccagca
     6421 atgcttctgc actccggggt ggacgtcgcc accctgagcg aggttggtgc aagctgcggt
     6481 tcgcgcgaca ccggactcgt agatcccgtt caagccgctg ccgtagtagg tgacgtagga
     6541 ggtgccatgc ggaagaatgt agcctccgcc cgcatccgcc aaaaagtcca ggtcgttgga
     6601 gaggatgtag caggacttcg cgatcccttc t
//
LOCUS       DASTKS010000778         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_570362, whole genome shotgun sequence.
ACCESSION   DASTKS010000778 DASTKS010000000
VERSION     DASTKS010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570362"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /gene="hisE"
                     /locus_tag="VFQ35_16115"
     CDS             <1..301
                     /gene="hisE"
                     /locus_tag="VFQ35_16115"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HET9932230.1"
                     /translation="ALEGIIQARSQSTAEKSYTRSLLDGGAAKIGAKLREEGGELAQA
                     IADESDDRVVSEAADVMFHLLVGLRLRGLSLREVEAKLNSRMGVSGHVEKASRKH"
     gene            517..2853
                     /locus_tag="VFQ35_16120"
     CDS             517..2853
                     /locus_tag="VFQ35_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932231.1"
                     /translation="MPTKVLVFESDAGFAGELRTELTRLDCEVNVVDDASVGLQTAAR
                     DRPDLILLAIELPRMNGFSVCNKLKRDPGLKDVPLIIMSTDSSEETFEQHRRLRTRAE
                     DYVHKPIAFGDLLTRIQAFVPIGKNASHVDDDDDAIVIEDDIVLEEGGGVVVADAELD
                     LETDKQTDAAFDSLVEGPRPSEPVEAGIEITEAEVLSAPPPSGSASPSSQPLEITDEE
                     VGRALSEYPPRVSSLPPRVSSIPARIATPAPASMRGSAIPKRVSTPAARLPSVAPRTD
                     EVARLAGEAERYRNRSAELEEELKTTRARITELEDVQRRASAREHELQRLERELEEVK
                     SKLTSGGKSMGNAREFLDLREQLNKKDKELIDFRDQLTHRDKELVAARDATLDLERKN
                     ADQSDRIVELERQTNDLSRNVEVLRNDKDAASKRAEDFKRKSEKLKTDLDAKLVEAAE
                     LKAQLDAELEARTVREAELEARAQADLEKAVLATESSERERAEKALAEARATAAQELE
                     AALNAERERGLQELTNAVNTKEALLTEQHNERIASFERANEETLLKLRAEHEQALADQ
                     SAAAAQHLAEREAELAAQAQRELARANDEGFAKLAALEAARVEQLAALEAARAEQVAE
                     RDGTIANLERDLAHRTSERDDARRDVDKKKLRIEGLETDVATTRDALDAARNNVKELR
                     ASLESTQQALQQKQAEAADLSQRLNQTQAALTTLEVQHAAQAGELQQTQAALEHDRSV
                     LQRAREKRDQDRASLERTKDALAAALQQVDDIEARTFD"
     gene            complement(2856..3641)
                     /locus_tag="VFQ35_16125"
     CDS             complement(2856..3641)
                     /locus_tag="VFQ35_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET9932232.1"
                     /translation="MIRRVVALACSNTEIVCALGCAHLLVAVDDHSDFPVGVVNALPR
                     VGPDLGIDVERVASLSPDLVLASLSVPGHERVVENLKRKGLPFIAPEPRSVEDVYANI
                     RQIGALLSVPERADALVAEMQRELAPAPPSASAPRILIEWWPKPVIAPGRDSWVNQVL
                     AAAGARNPLAERAVKSTPLSDEDAAALRPDASVISWCGVRLEKYRPDVVLRRPAWQGL
                     ELVERRRVFRISEAWLGRPGPRLVEGARALREIVRQLQAETPA"
     gene            complement(3638..>3927)
                     /locus_tag="VFQ35_16130"
     CDS             complement(3638..>3927)
                     /locus_tag="VFQ35_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932233.1"
                     /translation="GLLEGRSWASFHDRFSGSLRPSLEGTLSADSRAADAELVETLFG
                     EGGGEGGQPSELTDHLTEGFFPANLPHSYFCWELEPGVFHVIEGVKSVHEP"
BASE COUNT          792 a         1362 c         1272 g          501 t
ORIGIN      
        1 ggcgctcgaa ggcatcatcc aggctcgcag ccaaagtacg gcagaaaaga gctacacccg
       61 ttccttgctg gacggcggag ccgcgaaaat cggcgcaaaa ctccgcgaag agggcggcga
      121 gctcgcgcag gccatcgcag acgagagcga tgaccgcgtg gtcagcgaag ccgccgacgt
      181 catgttccac ctgctcgtcg gcctgcggct ccgtgggctt agcctgcgag aggtagaggc
      241 caagctaaac agccgtatgg gcgtcagcgg acacgtcgag aaagcctcgc gcaaacactg
      301 aagcggcgga gccgaggcgc gcgcgcgatc agcgtttccg gcaccgtacg cggtggttca
      361 aggtcgcacc ttggacgcga gcgggctcct gtgaagcgcg caaaatcaag cattaccggg
      421 agtttgaccc cacgagacgc gcccgatatc gtgacagttc gtgaggcagg aagtcgcgca
      481 ctcagccctg ccgcgaggtc ataggtaaac gacgcgatgc ccaccaaggt cctggtcttc
      541 gaaagtgacg ctggcttcgc cggcgagctc cgcacggagc tcacgcgctt ggactgcgag
      601 gtcaacgtgg tggacgacgc cagcgtcggc ctccagaccg ccgcacgcga ccgtcccgac
      661 ttgatcctgc tcgcgatcga gctgccgcgc atgaacggct tctccgtctg caacaagctc
      721 aagcgcgatc cggggctcaa ggacgttccc ctcatcatca tgagcaccga ctcgagcgaa
      781 gagacgttcg agcagcaccg gcgcctccgg acacgcgccg aagactacgt gcacaagccg
      841 atcgcgttcg gtgacctgct cacgcgcatc caagccttcg taccgatcgg taaaaacgcg
      901 agccacgtcg acgacgacga cgacgccatc gtcatcgagg acgacatcgt gctcgaagag
      961 ggcggcgggg tcgtcgtcgc ggacgccgag ctcgatctcg agaccgacaa gcagacggac
     1021 gcggctttcg attcgctcgt agaagggccg cgcccctccg agcccgtgga ggctggcatc
     1081 gaaatcaccg aagcggaggt gctctccgcc ccgcccccgt cgggcagcgc gtcgccgtcg
     1141 tcccagccgc tcgaaatcac cgacgaggaa gtcggtcgcg cgctctcgga gtatccgccc
     1201 cgcgtatcgt cgctcccgcc gcgcgtctct tcgattccgg ctcgaatcgc gacgccggca
     1261 ccggcttcga tgcgaggctc cgccatcccc aagcgagtct cgactcccgc ggcgcgcctg
     1321 ccttcggtcg ctccgcgcac ggacgaagtc gcgcgcctcg ccggcgaggc cgaacgctac
     1381 cgcaatcgca gcgccgagct cgaggaagag ctgaaaacga cgcgagcccg catcacggag
     1441 ctcgaagacg tgcagcgccg cgcgagcgcg cgcgagcacg agctgcagcg gctcgaacgc
     1501 gagctcgaag aggtcaagtc gaagctgacc tccggcggaa agtcgatggg caacgcgcgc
     1561 gagttcctcg atctgcgcga gcagctcaac aagaaggaca aggagctcat cgattttcgc
     1621 gatcagctca cgcatcgcga caaagagctc gtcgccgcgc gtgacgcgac cctcgacctc
     1681 gagcgcaaga acgccgacca atcggaccgc atcgtcgagc tggagcgaca gaccaacgac
     1741 ctttcgcgca acgtcgaggt gctgcgcaac gacaaggacg cggccagcaa gcgcgccgag
     1801 gacttcaagc gcaagagcga gaagctcaag accgacctcg acgcgaagtt ggtcgaagcc
     1861 gccgagctca aggcccagct cgacgccgag ctcgaggcgc gcaccgtgcg cgaagccgag
     1921 ctcgaagcgc gagcgcaggc cgacctcgag aaagcggtgc tcgccaccga atcttccgag
     1981 cgcgagcgcg cggaaaaggc tctcgccgaa gcgcgcgcca ctgccgcgca ggagctcgaa
     2041 gccgctctca atgcggaacg cgagcgcggc ctgcaagagc tcaccaacgc ggtcaacacg
     2101 aaggaagcgc tcctcaccga gcagcacaac gagcgcatcg cctcgttcga gcgcgccaac
     2161 gaagagacgc tcctcaagct acgcgccgaa cacgagcagg ccctcgccga tcagagcgcc
     2221 gccgccgcgc agcacctcgc cgaacgcgaa gcggagcttg cggcgcaggc gcagcgagag
     2281 ctcgcccgcg ccaacgacga gggcttcgcg aagctcgcgg cgctcgaggc cgcacgggtc
     2341 gagcagctcg cggcgctcga ggccgcgcgc gccgaacagg ttgccgagcg cgatggaacg
     2401 atcgcgaacc tggagcgcga cctcgcccat cgcacgagcg aacgcgacga cgcgcgacgt
     2461 gacgtcgaca agaagaagct ccgcatcgag gggctcgaaa ccgacgtcgc gacgacgcgc
     2521 gacgcgctcg acgctgcgcg caacaacgtg aaggagctcc gcgcctcact cgagtcgacc
     2581 cagcaagcac tgcagcagaa gcaggccgaa gccgcggatc tctcgcagcg cctgaaccaa
     2641 acccaagccg cactcacgac gctcgaggtc cagcacgcag cccaagccgg cgagctacag
     2701 caaactcaag ctgcgctcga gcacgaccgc agtgtgctcc agcgcgcgcg cgaaaagcgc
     2761 gaccaagatc gggcgagcct cgaacgcacg aaggacgcgc tcgcggccgc cttgcaacaa
     2821 gtcgacgaca tcgaggcgcg caccttcgac tgaactcaag ccggcgtttc ggcctggagc
     2881 tgacgaacga tctcgcgcag cgcccgcgct ccttcgacta gacgcgggcc tggtcgaccg
     2941 agccaggctt cgctgattcg gaacactcgg cgtcgctcca cgagctcgag cccttgccac
     3001 gccggacggc ggagcacgac gtcggggcga tacttttcga gccgcacgcc gcaccagctg
     3061 atgacgctgg catcggggcg gagcgcggcc gcgtcctcgt cgctcagcgg agtgctcttc
     3121 accgcgcgct cggcgagcgg gttccgagca ccggccgcgg cgagcacctg gttcacccag
     3181 ctatcgcggc ccggcgcgat cacgggcttt ggccaccact cgatgagaat acggggagcg
     3241 ctcgcggagg gcggtgccgg ggcgagctct cgctgcattt cggcgaccag cgcatccgcg
     3301 cgctcgggaa cgctcagcaa ggctccgatc tgacggatgt tcgcgtagac gtcttcgacg
     3361 ctgcgcggct cgggcgcgat gaatggcagc cccttccgct tcagattctc gacgacgcgc
     3421 tcgtggccgg gaacgctgag cgacgcgagt acgagatcgg gagagagcga cgcgacgcgc
     3481 tcgacgtcga tgccgagatc cggaccgacg cgcggcaatg cgttgacgac gccgacgggg
     3541 aagtcgctgt gatcgtccac cgctaccagc aagtgagcgc agccgagcgc gcagacaatc
     3601 tcggtgttgg agcaggcgag cgcgacgacg cggcgaatca cggctcgtgc accgacttca
     3661 cgccttcgat gacgtgaaag actccgggct cgagctccca acaaaagtag ctgtgcggca
     3721 agttcgccgg aaaaaatcct tccgtcaggt gatccgtaag ctcactcggc tggcctccct
     3781 cgccgccacc ttcaccgaac agcgtctcga cgagctcggc gtctgcggcg cgcgaatcgg
     3841 cgctgagcgt gccctcgagc gacggccgca agctgcccga aaaacgatcg tgaaagcttg
     3901 cccacgagcg cccttcgagc aaaccag
//
LOCUS       DASTKS010000779         5382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_570364, whole genome shotgun sequence.
ACCESSION   DASTKS010000779 DASTKS010000000
VERSION     DASTKS010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570364"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VFQ35_16135"
     CDS             <1..1485
                     /locus_tag="VFQ35_16135"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9932234.1"
                     /translation="LGLLPSIDDSSVAAELVAVLGKSHDERALPALLELLRSGDTDVH
                     ASAIRGLGELPQREAAPILCSLLKVGSANEFDQAAEALARLAPATLVQELASLLESNN
                     AARRMLAAVGLGRMLPNAEARRMVLGLLDSDEPPLVAVAARFLSRAKEPGIVDKLLYL
                     ARSDVPGVRMAAVRALGDTVDEGARAALKSLYDEQSVPLHVVLTNLAVDSSPEAQSAL
                     LGHLSEPGEDAIGVIQSFVPSLPDSTRRAIAARVATERDPERYRSFVEALSASADPDL
                     VPLLREALREHSGVARRAATRALISLDVPEAEQLLDGLLNSDDPDEQSLGFESLTERG
                     DAESLTRLAALAGRKLEDESLDAFEASRAFSVLKFRSPERAAQLLDAAFSRGPASTRA
                     RMLSLGNGLGQLAIPTCLRALTSDDSGVVEAAIDLAQSLGGERVSRALLGIARDPRRD
                     EAERRSAANALKSIGGPVAREQASLLGSLLEEEEEAFECPWSEP"
     gene            1464..2711
                     /locus_tag="VFQ35_16140"
     CDS             1464..2711
                     /locus_tag="VFQ35_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932235.1"
                     /translation="MPLERAVSATRVWMLLALAASAMLLFAAARAPRIAPPRHDPCSA
                     RATPETTTEPTRLDAPAFERAPRPKSESPLSLTVLEERASKARDSELSQAMRTIAEQG
                     GDGALAALTRLSRAKRGSVARAALVALDQLDTEAARNYMLAALGGDTPAAALEYFKDA
                     VEPRAIPRLEQLAQTSGTELTEAAVAALVTQGGPAHASLTRLVQSDGEAREAVIANDA
                     ARLALRPTLRTTCIAALRSGTAIQSCWDLLSSDRSTAALAALSSAAHDRALGQAAIQA
                     LSHRGDPAAVAVLLELDRRGEPWAACEARQVVLSDVDSRSLTPLLSMLRDAPRTEVIR
                     GLLELGAEEAARALDARLRSSESRVRARAVTLLAEFGPKDYMRSLRSLRNDQDAAVAS
                     LAEASLERLAVQSRSYSLHAYSQ"
     gene            2946..4112
                     /locus_tag="VFQ35_16145"
     CDS             2946..4112
                     /locus_tag="VFQ35_16145"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET9932236.1"
                     /translation="MDTNLWMAGVAALVIALVGWIWSGRPQTKRAASNDPSKQTSLPK
                     QKPVRKARPPALPTTTLDEDVEITLIATAPIADIVRKVRAEHAAALQQPGDKDSPAIS
                     AEEPREPDDQRPSRVELTYEAEAEAEEVTSPVVRILIHAAGDSDRGRTRAKNEDSMLV
                     FPERSVFAVADGMGGHAGGEVASSVAVDVLRDAFERSVFEARTEASVEVPRRGRELAC
                     AIQMANEAIYALAQDDPALKKMGTTVVAARFSPNKQRVYIGHVGDSRCYRLRGEDFRQ
                     LTKDHTMSTFGVTGPRGNDLYQAVGVQPTLSIDLIVDKPSENDLYLLCSDGLSKMVSN
                     EEIREVLLAEADLEASVYGLMELANDRGGRDNITLILVKVIERPVKVLSPEQRS"
     gene            4216..>5382
                     /locus_tag="VFQ35_16150"
     CDS             4216..>5382
                     /locus_tag="VFQ35_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9932237.1"
                     /translation="MKRSTRPFARVLPLLLSACQKSPTADRKHVEPTGADATSAGAGH
                     GAERVSAASASAAPPVPFEPRALALAWNGSATLPFDAQRLGADHTIFARFMPAYEGAY
                     RGILIADQTGSYRLGMAPYASLNQPAMEVVIGGSTFTTPLPQPLLSTLDVTRRAEPPL
                     TARWLSLAVSVRGTQATVYVNGASAGTLDVGERHLAGPLVLGRLARAEAVQDQFYGLV
                     DDVAIFERALAPAEVLALNRLPRFDSRLDALLAVADFDRTRASSRSLEPTLSGAATLS
                     AVSGRREPRPDAARLPSPQLPVRFSLPFAPGQTWMVIQGANSVGSHHDTAAFAIDFQR
                     VDPLVVEHNPEHRPGASAALSAGQSFLAAAEGRVVSLVDCFRENDRGCSGVHVDP"
BASE COUNT          867 a         1833 c         1801 g          881 t
ORIGIN      
        1 ctcgggctac tgccgagcat cgacgattcg agcgtcgcgg cagagctcgt ggccgtactc
       61 ggaaagtcgc acgacgagcg cgcgctgccc gcgttgctcg agctcttgcg gagcggcgat
      121 accgacgtgc acgcgagcgc gattcgcggc cttggcgagc tgccgcagcg cgaggcggca
      181 ccgatcttgt gctcgctgct gaaggtcggt tccgccaacg agttcgacca ggcggcggag
      241 gccctcgctc gtctcgctcc cgcgactttg gtgcaggagc tcgcgagtct cctcgaatcc
      301 aacaacgctg cccgtcgcat gctcgcggcg gtcgggctcg ggcgcatgct cccgaacgcg
      361 gaggcgcgac gaatggtgct cggtctcttg gattcggacg aaccgccgct ggtcgcagtt
      421 gcggctcgtt tcctctcccg cgcgaaagag cctggcatcg tcgacaagct gctctacttg
      481 gcgcgctcgg acgttcccgg ggtgcgaatg gcggccgtgc gtgcgctcgg agacaccgtg
      541 gacgaaggtg cgcgagcggc cttgaagtcg ctctacgacg agcagagcgt gccccttcac
      601 gtcgtgttga ccaacttggc ggtcgactcg tcgccggagg ctcagtccgc gctgctcggt
      661 cacctgagcg agcccggaga agacgccatc ggcgtcatcc agtcgttcgt cccctcgtta
      721 ccagactcga cgcgacgagc gatcgctgcg cgggtagcga cggagcgcga ccccgagcgc
      781 tatcgttcct tcgtcgaggc gctctcggct agcgccgatc ccgacctcgt gccgctgttg
      841 cgcgaggcgc tgcgcgaaca ctcgggagtg gcccgacgcg ccgcgacgcg cgcactgatc
      901 tcgctcgacg tgccggaggc ggagcagctg ctcgacggcc tgctgaacag tgatgatcca
      961 gacgagcaaa gcctaggctt cgagtcgttg accgagcgag gcgacgctga atcgctcacc
     1021 cggctcgcgg cgctcgcagg ccgaaagctc gaggatgaat cgctcgatgc gttcgaggct
     1081 tcccgcgcgt tctccgtctt gaagttcagg tctcccgagc gagctgcgca actcttggac
     1141 gcggctttct cgcggggccc cgcgtcgact cgcgcccgca tgctctcgtt ggggaacggc
     1201 ctcggccaac tagccatccc gacctgcctg cgggcgctca ccagcgacga cagcggcgtg
     1261 gtcgaagctg cgatcgactt ggcgcagagc ctcggcggcg agcgcgtgag ccgcgcgttg
     1321 ctcggcattg ctcgcgatcc acgccgagac gaggccgagc ggcggtccgc ggcgaacgcg
     1381 ctgaagagca tcggcgggcc cgtcgcgcgc gagcaagctt cgctgctcgg gtcgttgctg
     1441 gaggaagaag aagaggcgtt cgaatgccct tggagcgagc cgtgagcgcg acgcgcgttt
     1501 ggatgctgct ggcgctcgca gcgagcgcca tgctgctctt cgcagccgca cgagcgcccc
     1561 ggatagcgcc gccgcgacat gacccctgca gcgctcgcgc gacaccggag acgacgaccg
     1621 aacccactcg tctcgacgcg ccggctttcg aacgagctcc gcgcccgaag tccgagtcac
     1681 cgctgtcgct caccgtccta gaagagcgag cgtcgaaggc gcgcgactcg gagctttcac
     1741 aggcgatgcg cactattgca gagcagggcg gggacggtgc gctcgcggcg ctcacgcgac
     1801 tctcgcgagc aaagcgcggc tcagttgcgc gcgcagcgct cgtcgcgcta gatcagctgg
     1861 atacggaagc tgcccgcaat tacatgctgg cggcgctcgg gggcgacact cccgccgcgg
     1921 ctctcgagta cttcaaagac gcggtcgagc cacgtgcgat ccctcgcctc gagcagctcg
     1981 cgcagacgag cgggacggag ctgacggagg cggccgtggc cgcgctggtc acgcaaggcg
     2041 gtccggcgca tgcctcgctg actcgcctcg tgcagagcga cggcgaagcg agggaagccg
     2101 tgatcgcgaa cgatgcggct cgactcgcgc tccgaccgac gcttcgaacg acgtgcatcg
     2161 ccgcgttgcg ctcggggacg gcgattcaaa gctgttggga cctgttgtcg agtgatcgct
     2221 cgacggcagc gctcgcggcg ctgtcttctg ccgcgcacga ccgagccctc ggccaggcag
     2281 cgatccaggc tttgagccac cgcggagacc cggccgcggt cgcggtgctg ctcgagctcg
     2341 atcgtcgcgg agaaccttgg gccgcgtgcg aggcgcggca agtcgttctc tccgatgtcg
     2401 actcgcggtc cttgacgccc ctcctctcca tgctgcgcga tgctcctcga acggaggtca
     2461 tccgcggctt gctcgagctc ggcgcagaag aggcagcgcg agctctcgac gcgcgccttc
     2521 ggagcagcga gagcagagtg cgcgcccgcg cggtgacgct gctcgcagag ttcgggccga
     2581 aagactacat gaggtcgtta cggtcgctac gaaatgacca agacgccgcg gtcgcttcgc
     2641 tggccgaggc gtcgctcgag cggctcgcgg tccaatcacg gtcctactcg ctgcacgcgt
     2701 actcgcaatg agtgcggcgc tcgcgtttcc cgctcgccgc tcgatcaggt gcggcggggt
     2761 acgctctggt gcgcattcgc gtccgtgtac tcgcggtgcg ttcggccgag ccagcccgag
     2821 ggtacctgct ctcgcgggcc gaagtcgcgt gcgcgtatcc taccttggac ccgtcgagcg
     2881 catcgacgcg catcgaatgt tatgagagct agctttctgg acccaaaatc ccgcaatatt
     2941 ttccggtgga cacgaatctt tggatggccg gcgtcgccgc gctcgtgatc gcgctggtcg
     3001 gctggatctg gtcggggcgc ccgcagacga agcgcgcagc gtcgaacgac ccaagtaagc
     3061 agacgagttt gccgaaacag aagccggtgc gtaaagcgcg acctccggcg cttccgacta
     3121 cgactctcga cgaggacgtc gagatcacct tgatagcaac cgccccgatc gcggacatcg
     3181 ttcgcaaagt gcgggccgaa cacgccgccg cactgcagca accgggcgac aaggattcgc
     3241 cggccatctc cgccgaagag ccgcgcgaac cagacgacca gcgcccgtcg cgggtcgagc
     3301 tgacctacga agcggaggcg gaggccgaag aggtcacgtc tcccgtcgtc cggattctca
     3361 tacacgccgc gggtgacagc gatcgcggcc gcacgcgcgc caagaacgaa gacagtatgc
     3421 tcgtcttccc ggagcggtcg gtcttcgccg tcgcggacgg gatgggcggc cacgccggcg
     3481 gcgaggtggc aagctccgtc gcggtggacg tgctgcggga cgccttcgag cgcagcgttt
     3541 tcgaggctcg aaccgaggcc tccgtcgagg ttccccgccg cggtcgcgag ctcgcctgcg
     3601 ccatacagat ggccaacgaa gcgatttacg cgctcgcgca ggacgacccg gcgctgaaga
     3661 agatgggcac caccgtcgtg gcggcgcgat tctccccgaa caaacagcgg gtttacatcg
     3721 gtcacgtcgg cgatagccgc tgctaccgac tgcgcggtga agacttccgt cagctgacca
     3781 aagaccacac catgagtacg ttcggcgtga cgggaccgcg cggaaacgac ctctaccagg
     3841 ccgtcggcgt gcaacccacg ctcagcatcg atttgatcgt cgacaagccg agcgaaaatg
     3901 acctctacct cctgtgctcg gacggcctga gcaaaatggt gagcaacgaa gagatccgcg
     3961 aggtgcttct cgccgaagcc gacctcgagg cgtccgtata cggcttgatg gagcttgcca
     4021 acgatcgcgg cgggcgcgac aacatcaccc tcatcttggt caaagtcatc gaacgccccg
     4081 tcaaggtgct ctcgccggag caacgaagct gagcgagcgc tgacgcgcat ccgcggcgaa
     4141 tgacgccgcg cacatgcggc tgacgtcgaa ccgcatccgt cgcgacatca acggccgctc
     4201 tcacgaatcg tcgagatgaa acgcagtacg cgcccattcg ctcgtgtgct gcctttgctg
     4261 ctctctgctt gccagaagtc gcccacggcc gaccgcaagc atgtcgagcc gaccggcgcc
     4321 gacgcgacca gcgcgggcgc cggccatggt gcggagcgag tctccgccgc gtcagcgagc
     4381 gccgcgcccc cagtgccgtt cgagccccgc gcgttggctc tcgcgtggaa cggctcggcg
     4441 accctcccat tcgatgcgca gcgcctcggc gccgatcaca cgatattcgc gaggttcatg
     4501 ccagcgtacg agggcgctta tcgcggaatc ctcatcgctg accagaccgg cagctatcgg
     4561 ctgggcatgg ccccctacgc atcgctgaat caaccggcga tggaggtggt aatcgggggt
     4621 tcgacgttca ctaccccgct acctcagccg ctcttgtcga cgctcgatgt gactcgccgc
     4681 gcggagccac cgctcacggc gcgctggctt tcgcttgcgg tgagtgtccg cgggacccag
     4741 gcgaccgtat acgtaaatgg cgcttcggca ggtacgctcg acgtggggga acgtcacctg
     4801 gctgggccgc tggtcctcgg tcggctcgca cgcgcggagg ccgtccagga ccagttctac
     4861 gggctcgtgg acgacgttgc gatcttcgag cgagctctcg ctccggccga agttttggct
     4921 ctgaatcgcc tgccgcgttt cgactcgcgc ctcgacgcgc tcctggccgt cgccgacttc
     4981 gatcgaactc gcgcgagctc gcggtcgctc gagccgaccc tgagcggcgc cgcgacgctc
     5041 tccgcggttt cgggaaggcg cgagcctcgc ccggacgccg cgcggctgcc gtcaccgcaa
     5101 ctgccagtgc gtttcagctt gcccttcgca ccggggcaaa cctggatggt catccaaggc
     5161 gcgaacagcg tcggctcgca tcacgacacg gcggcgtttg cgatcgactt tcagcgcgtc
     5221 gatccgctcg tcgtggagca caatcctgag caccgccccg gtgcgtcggc cgcgctcagc
     5281 gcggggcagt catttctagc ggcggcggag ggccgcgtcg tctcgctcgt cgattgcttt
     5341 cgagagaacg accgaggttg ctccggggtg cacgtcgacc ct
//
LOCUS       DASTKS010000780         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_124738, whole genome shotgun sequence.
ACCESSION   DASTKS010000780 DASTKS010000000
VERSION     DASTKS010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124738"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VFQ35_16155"
     CDS             <1..845
                     /locus_tag="VFQ35_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932238.1"
                     /translation="ERAKVRLRESLVQGLSRLASAEPANWRRVLRRHNEALLGAAISD
                     ERLFQLLSKELTVPTSMGDMTVPAVLEQSRGTVHVRDLDTSPHQELLFRALNVPVVDG
                     ARYGAHSFCRLYCERIAGKLVVLGTQQGDRELFKEANLGVAERVRLNELFGAPDRQVL
                     VSRFAPAYLPFIAVKDREVELKRRIEAEEADKRMGSAVLSLARKFTATIEGAAVYRFH
                     VNVDSPVIQALLAAPPARAEFALRLLAPLGVLLGEAGPSGATETALKGFSDAICTVLS
                     EAKT"
     gene            842..>2673
                     /locus_tag="VFQ35_16160"
     CDS             842..>2673
                     /locus_tag="VFQ35_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932239.1"
                     /translation="MNIWEWVHDAVRELRGNGQERLAELIDDVSTACCDGQHDRVENI
                     VPEALALARAAKNPWIELFIRHWLLQSRVLHRHDVSRDTMKEAVSLIEFASRPETRDC
                     PQGICAVQDLASAYGLVDGVGYAEERLAVTEEALGRIDASWPCFDCVSSERASALLDL
                     RRHREALEFCDAQLAQSPRSDSGIMGNRFRALCALGRFADAERVAANIDIENAGESGA
                     VRKALYEALALAKLGQAGAARDKLPALSRLEPEHFVDFLRCVRALGVGDDRPNTWRIE
                     RAVADMSRVLQRYESYFTLTRVHKIAVELAFQRGARASAERHIAAARKAAEHLRDPRW
                     VETSLAPLADRLRALPAPTVPNDADELFAALGEDPERDLALLECAGLSEVRFERARSR
                     ALDALGMSREALDVLVAAFERWPDDSRLLLHLLELLREQGEHARLESLCEEATGPART
                     ASRIILARSRVARGEPELARRACLEALELDPTQDEARSLLATLLRDAGEFEPALEHMN
                     ELVQRHEPGDLDWDRMLIATLAGRWDTVRDSAKRLGLPVEDAESGPIDDVWGPCRIRF
                     RLADGSERAYFAWRTGPVTARVDEVIHPALEQHHGDVVVFDATP"
BASE COUNT          432 a          863 c          915 g          463 t
ORIGIN      
        1 tcgagcgagc gaaggtgagg ctgcgcgaat cgctcgtgca agggctctcg cgtctcgcgt
       61 cggcagagcc agccaactgg cgtcgcgtcc tccgccgaca caacgaggcc ctgctcggcg
      121 ccgcaatctc cgacgagcgc ctgtttcagc ttctcagcaa ggagctcacc gtccccacca
      181 gcatgggcga catgaccgtg ccggcggtgc tggagcaatc gcgcggaacc gtgcacgtgc
      241 gcgacctcga tacgtcgccc caccaggagc ttttgttccg cgccttgaac gtccccgtag
      301 tggatggcgc acgctatggg gctcactcgt tttgtcgctt gtactgcgag cgcatcgccg
      361 ggaagttggt cgtcctcggc actcagcaag gcgaccgcga gctcttcaag gaggcgaact
      421 tgggcgtcgc cgagcgcgtc cgcctgaatg agctcttcgg cgcgcccgac cgccaggtgc
      481 tggtcagtcg atttgcgccc gcatacctcc ctttcatcgc ggtaaaggac cgggaggtcg
      541 agttgaaacg ccggatcgag gcggaagagg cggacaagcg catgggctcc gccgtgctgt
      601 cgctcgctcg aaagttcacc gcgaccatcg aaggagccgc tgtctatcgt ttccacgtga
      661 acgtggactc accggtgatc caagcgttgc tcgcggcgcc ccccgcgcgc gccgagttcg
      721 cgctgcgctt gctcgcgccg ttgggggttt tgctcggcga agcggggccg agcggcgcga
      781 ccgagaccgc tttgaaaggc ttcagcgacg cgatttgcac cgtgctttcg gaggccaaga
      841 catgaacatc tgggagtggg ttcacgacgc agtgcgggaa ctgcggggaa acgggcaaga
      901 gcgcttggcc gagctgatcg acgacgtatc gaccgcctgc tgcgatggcc agcacgatcg
      961 cgtggagaac atcgtgcccg aggcgctggc gctggcgcgc gcggcaaaaa atccgtggat
     1021 cgagctgttc attcggcatt ggctgctgca aagccgtgtg ctgcaccgtc acgacgtgag
     1081 ccgtgacacg atgaaggaag cggtatcgct catcgagttc gcgagccggc cggagacacg
     1141 cgactgcccg caaggcatct gcgcggtgca agatctcgcg agcgcctacg gtctcgtgga
     1201 cggtgttggg tacgcggaag agcggttggc cgttaccgaa gaggcgctcg ggcgcatcga
     1261 tgcgtcgtgg ccatgcttcg actgcgtgag ctccgaaagg gcgagcgcgt tgctcgacct
     1321 tcgccgccat cgagaggcgc tcgaattttg cgacgcacag ctggcgcagt cgccgcgcag
     1381 cgatagcggc atcatgggca atcgctttcg cgccctgtgc gcgctcggtc gattcgccga
     1441 cgcggagcgt gtcgccgcga acatcgacat cgagaacgcc ggtgaaagcg gggcggtccg
     1501 caaggcactc tacgaagcac tggcgctcgc gaagctcggc caggccggtg ccgctcgcga
     1561 caagctgccg gcgctctctc gactcgagcc cgagcacttc gtcgattttc tgcgctgcgt
     1621 gcgagctctt ggggtgggtg acgaccgccc caacacttgg cgtatcgaac gcgccgtggc
     1681 ggacatgagc cgagtcctcc aacgctacga gtcgtatttt actctgacgc gcgtccacaa
     1741 gatcgccgtc gaactcgcgt ttcagcgcgg cgcccgcgct tccgccgagc gtcacatcgc
     1801 ggccgctcgg aaagccgcgg agcacttgcg cgatcctcgc tgggtggaga cttcgctcgc
     1861 gccgctcgcg gaccgacttc gcgcgctgcc cgcgccgacg gtaccgaacg atgcggacga
     1921 gctcttcgcc gcgctcggtg aggacccgga gcgagacctt gcgctgctcg agtgcgccgg
     1981 attgtcggag gtccgcttcg agcgagcgcg ctcgcgtgcc ctcgacgctc tcggcatgtc
     2041 gcgcgaagcg ctggacgtgc tggtggcagc tttcgagcgt tggccggacg actcgcggct
     2101 gctcttgcac ctgctcgaac ttctgcgcga gcagggcgaa catgcgcggc tcgaatcact
     2161 ctgcgaagaa gcgactggcc cggcgcgcac cgcttcgcga atcatcctcg cccggagccg
     2221 agtcgcccgg ggcgaacccg agctcgcgcg tcgagcgtgt ctcgaggcgc tcgagttgga
     2281 tccaacgcag gacgaggcgc gctcgctact cgcgacgttg ttgcgagacg cgggagagtt
     2341 cgagccagcg ctcgaacaca tgaacgagct ggttcagcgt cacgagccgg gcgatctgga
     2401 ttgggatcgc atgctgatcg cgaccctggc cggtcgttgg gacaccgtgc gcgactcggc
     2461 gaagcgcctc ggattgccgg tcgaagacgc agaaagcggg ccgatcgacg acgtgtgggg
     2521 tccgtgtcgg atccggtttc ggttggcgga cggcagcgag cgcgcttact tcgcgtggcg
     2581 caccggtcct gtcacggctc gcgtcgacga ggtaattcat ccggcgctcg agcagcatca
     2641 cggcgacgtc gtcgtgttcg acgccacgcc ttt
//
LOCUS       DASTKS010000781         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1464298, whole genome shotgun sequence.
ACCESSION   DASTKS010000781 DASTKS010000000
VERSION     DASTKS010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464298"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1738)
                     /locus_tag="VFQ35_16165"
     CDS             complement(167..1738)
                     /locus_tag="VFQ35_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9932240.1"
                     /translation="MQSIDQRHYDVIIIGSGAGGGTLAYALAASGKRILLLERGDFVK
                     REKGNFDSEVVNLEARYQTKESWRDSRGEPLHPHTNYCVGGNTKFYGAALFRMRERDF
                     GEVKHKGGISPRWPISYADLEPYYARAEQLYQVHGERGADPTEPPASTGYPFPAVSHE
                     PRVQGLHDDWGNAGLRPFHVPLGVMLNEGDPAQSACIRCDTCDGFPCLLRAKSDAETC
                     AVRPALAHPNVSLLTQALVTRLETSASGREVTGVWVERRGERFRLSANIVVVSAGAIN
                     SAALLLRSASSRHPHGLANGSGVVGRHYMGHINSVLLALSLCPNPTVFQKTLALNDFY
                     FGDDEFPYPMGHISFVGKLDVHALRAGAPAIAPGWTLDLMARHSLDFWLTSEDLPDPN
                     NRVTLDAQGNIVLSYTPNNLEGHEQLQARLKRLMRRQKCQIHGADCHQGLFARNLFVG
                     NRIPLAGVAHQNGTVRFGEDPQSSALDPNCKAHELDNLYVVDASFFPSSSAVNPALTI
                     VANALRVADHLLDRL"
     gene            1904..2704
                     /locus_tag="VFQ35_16170"
     CDS             1904..2704
                     /locus_tag="VFQ35_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9932241.1"
                     /translation="MAETKLKILVTGASGAVGFAVARALRQRGHQVRGFDREPARVEG
                     AHTTADLRDFESLSQAVSGVDLVVHCAAVPDRQDFPTELVPTNIVGTHLVFEAARLAK
                     VSRVIYASSIRVVGGLDWGSGTIGVGAGLVPGDHYGVSKATGELLARMYAERFGLSVL
                     SARLGWFVRNLGEAELFETVRYARRIYLSHRDAEAFFVRAAEASWSGYSAVFVTSRND
                     GDSGFDLEPVRNLLDFEPADTWPSGSTWSDDMRFESPTFGPSVRPSGR"
     gene            2920..3321
                     /locus_tag="VFQ35_16175"
     CDS             2920..3321
                     /locus_tag="VFQ35_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9932242.1"
                     /translation="MKSGDGSAEQSSRTETMLPPSSGVDEYTFPVAGDTTQTLSFRVT
                     TAPPSVDFTLGERSVLKAIAAGMTNDEIAEERGSSRRAVMNLVRSICAKLNLANRAEL
                     ERHLAAEAGCVSAQDLRASNSLGGLAREKIL"
BASE COUNT          580 a         1060 c         1105 g          621 t
ORIGIN      
        1 cggagcagca cccgagtttg gtccgataac cgtcgagcgc gatgagtgat gcagagctct
       61 tcacgcacga gctcttgacg gtctccgtat gcgtccagcg cagggtcttg atgcgacgcc
      121 accacgcacg gaccctacac cgccggcggg cccgcggggc gccaattcaa aggcggtcga
      181 gcagatggtc agccacccga agcgcgttgg caacgatggt gagcgcaggg tttacggcag
      241 agctcgaagg aaagaaactg gcgtcgacga cgtagagatt gtcgagctcg tgggctttac
      301 aattggggtc cagagcggac gattggggat cttcaccgaa gcgcacggtg ccattttgat
      361 gcgccacccc cgcgagcggg atgcggttac ccacgaacag gttccgcgcg aagaggccct
      421 gatgacaatc cgcgccgtga atctggcact tttgacggcg catcaagcgc ttcagtcgcg
      481 cttgaagttg ctcgtgccct tcgaggttgt tgggggtgta actcaatacg atgtttcctt
      541 gggcgtcgag cgttacccgg ttgttcgggt ccggtaagtc ctccgaggtg agccagaaat
      601 cgagcgagtg gcgcgccatg aggtcgaggg tccaaccggg cgcaatggcg ggcgcacccg
      661 cgcgcagcgc gtgcacgtcg agcttcccga cgaacgaaat gtgccccatc gggtacggaa
      721 actcgtcgtc cccgaagtag aaatcgttca gcgcgagcgt tttctgaaac acggtcgggt
      781 tcgggcacaa cgacagcgcc agcagcaccg aattgatgtg gcccatgtag tgccgcccga
      841 cgactcccga tccattcgcg agtccgtgcg gatgccggct gctcgcagag cgcagcaaga
      901 gcgccgccga attgatggcc cccgccgaca cgacgacgat gttcgcggag aggcgaaagc
      961 gttcgccgcg gcgctccacc caaactccgg tcacttcacg ccccgaagcg ctggtttcta
     1021 gccgcgtcac gagcgcttgg gtcagaaggc tcacgttcgg gtgtgcgagc gcaggtcgga
     1081 ccgcgcacgt ctcggcgtcg gacttcgcgc gaaggaggca cggaaagccg tcgcacgtgt
     1141 cgcaacggat gcacgcgctc tgcgctgggt cgccttcgtt cagcatcact ccgagcggca
     1201 catgaaaagg ccgcagccct gcgttccccc aatcgtcgtg gaggccctgg acgcgaggct
     1261 cgtggctcac cgcggggaaa gggtagcccg tgctcgcggg tggctccgtc ggatccgcgc
     1321 cgcgctcgcc atgcacttgg tagagctgct cggcgcgcgc gtagtacggc tcgagatccg
     1381 cgtagctgat gggccaacgc ggcgagatcc ctcccttgtg cttcacttcg ccgaagtccc
     1441 gctctcgcat tcggaagagc gccgcaccgt agaacttcgt attcccgccg acgcagtagt
     1501 tcgtgtgcgg gtgaaggggc tcgccgcgcg agtcacgcca cgactccttc gtttggtagc
     1561 gggcttcgag gtttacgacc tcggagtcga agttgccctt ctctcgcttg acgaagtcgc
     1621 cgcgctcgag gaggaggatg cgcttgcccg aggcggcgag cgcatacgcc aatgttccgc
     1681 ctccagctcc gctgccgatg atgatcacgt cgtagtgtcg ttggtcgatg ctctgcatgt
     1741 ggccctccgc gcgaattcag cgctggcgta agtcgcgcgt ttccgcgcaa aaactgcgag
     1801 ctttcgctca gctagcgcgc tcgatgagta ctcactcgca ccggaagtac ggagatgacc
     1861 tcgaccggtt acctcgttgc ttctatcctt cccgcaaact tcgttggccg agacaaagct
     1921 caaaattttg gttaccggcg cctcgggtgc cgtaggtttc gcggttgcgc gcgcgttgag
     1981 gcagcgcggg caccaggtgc gcggcttcga tcgcgagcca gcgcgggtag aaggcgccca
     2041 cacgacagcg gacctacgcg acttcgagtc gttgagtcag gccgtcagcg gcgtagacct
     2101 cgtcgtgcat tgcgcggccg tgccagaccg tcaggacttc ccgaccgagc tcgttcccac
     2161 gaacatcgtc ggtactcatc tggtgttcga agccgcgcgc ctcgccaagg tttcgcgcgt
     2221 catctacgcg agcagcatcc gggtcgtggg cggcctcgac tggggcagcg ggaccatcgg
     2281 agtcggagct gggctggttc cgggcgacca ctacggcgtg agcaaggcga ccggggagct
     2341 tctggccagg atgtacgccg agcgcttcgg cctgtcggtg ctctcggcgc ggctcggctg
     2401 gttcgtgcgc aacctgggcg aggcggagct gttcgagacc gtgcgctacg cccgccgaat
     2461 ttacctgagc caccgcgatg cagaggcctt cttcgtgcgc gcagcggagg cctcgtggag
     2521 cggctactcg gcggtgttcg tgacgagccg aaatgacggg gactctggct tcgacctcga
     2581 gcctgtccgg aatcttctcg acttcgagcc ggccgacacc tggccgagcg gctccacttg
     2641 gtccgatgac atgcgcttcg aatccccaac gttcggcccc agcgtgcgcc cgagcggtcg
     2701 ctaaaaacgc cgagaatagc ccgccttcgt caatggtttg gcgcggcggc ttctgcggag
     2761 gcggcttcga ggcgacctgg cgcaccgcgt cgactaacac tctcgcggcg attttcgtcc
     2821 tattgagttc tcgaaagccg ataatgttgc ctaggcgagc gatgttgaag cagaaatagc
     2881 ctcgtcgtat cgtcctcgtc tcgagcggag gaattccgaa tgaagtctgg cgatgggagt
     2941 gccgaacaat cgagccgcac ggagaccatg ctgccgccgt catccggagt cgatgagtac
     3001 acgtttcccg tcgctggcga tacgactcag acgctgtcgt tccgcgtgac caccgcgccg
     3061 ccgtccgtcg atttcacgct cggcgagcgc agcgtcttga aggccatcgc ggccggcatg
     3121 accaacgacg agatcgctga agagcggggt tcctctcggc gcgcggtgat gaatctggtt
     3181 cgctcgatct gcgcgaagct gaacctcgcc aaccgtgccg agctcgagcg ccacttggcc
     3241 gcggaggccg gctgcgtctc ggcgcaagat ctgcgagcgt cgaacagcct cggcgggctc
     3301 gctcgcgaga agatcctcta ggatctgcgt tgcgtcgaac ggcgcgcgca attccgagac
     3361 acgcgc
//
LOCUS       DASTKS010000782         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1464802, whole genome shotgun sequence.
ACCESSION   DASTKS010000782 DASTKS010000000
VERSION     DASTKS010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464802"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..2062)
                     /locus_tag="VFQ35_16180"
     CDS             complement(245..2062)
                     /locus_tag="VFQ35_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932243.1"
                     /translation="MHESTLLAERYSVDAVLKSEAGAHRWLGTEAKSGRRVLLSWVSK
                     ARAEELAAARGLHQPHLAELLDLVRDADAAKLPGGSVLEPGGVIAVAEYVPGRTLQQL
                     LTERVSPVKAVAWLLRLIDAVRELHARGGVHGAISPFTVVAEPEGRAIAPVLTQLSAP
                     ALGAYCSPERLKGGAATAADDVWALHATLYALLAGKLPFDATERDALVKQTLITKPQA
                     LLQLGVNEPALWEIISRGLVGERRLRVTELAELTKSLDDWERDPRTMPARRSPPRQAP
                     PRLDAGSKVAADVVLFDVGALPQDFGASEPVRPPPRPKIASASGTGPHRAMPPPLPAE
                     VRGSVNAPSLGDLASQLPRAPTPPPLPPSPPSVVARISKRLSFNPFERKRRVWPLVVL
                     AASAGGLAVYVAIATTQDAPAAPPPVEAAPAAAPRARAPEKPKRSAAEERESCVRSYF
                     PERQIEANLDFAFLCEDGDFREVAHKVFARAKQQPLTAAAVTDKVKDAAGTGLGWYEL
                     PATGIMRKACCASSPPVTLPETPGWCEQLQSAVRQIADDSARAEDLAPAARAYDKAVT
                     CLFANRISRPYSYDSVPNSAQRAAFQHFLSQAAISEARR"
     gene            complement(2639..4954)
                     /locus_tag="VFQ35_16185"
     CDS             complement(2639..4954)
                     /locus_tag="VFQ35_16185"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932244.1"
                     /translation="MSFGPGLRRSLVHRAALLDWLPPSERVGLLFGWGALAALGFLPQ
                     LGGPGYDSALVAGIVLPSTAAVSVALGSLRLERSLAASLVHALHFGLVLVAGAATLTL
                     LHGLRVGMCDPKEGFALYGLGPGVGALMGCATGALAAALAKRLRPNSRRVVAVLFALG
                     GPLAGIALSLVRFYTSPMVFAYDPYFGYFSGPLYDTVIASLWPLASYRLGSLATLIAL
                     LAWSVALDARGNRGVFRTLLDRKNAALIGVGATVASLVVTVSGTELGHFSTAGSIERA
                     LGRRLTSGRCDVVYSSAVLENDARRIGRECHMHLAQVERFFDVSGPERVRVLLFANDA
                     EKGRLMGAAHTYIAKPWRGEVYIQAAGYPHPVLGHELAHVVAGSFGRGPFKVAGLLSG
                     LVPDPGRIEGVATAASPDENDALTLEEWAAAMQRLALLPKLDSLFRLTFFGHNAAQAY
                     TAAGAFVRYLHGEFGAATVRRWYGGETLESVTQVSLGELEARWHAALKHISLSDAVLA
                     NARARFERPAFFARRCPRVIDRENAEANAHLAASDLTGAREGFHEVLRLDPHDASARI
                     GLATCAARANDLQRAAVSFGKLSEARDMAPWARLSAQEARADVLLRQGQTEQAFDAYD
                     ALAQKLVDEDRLRTLDVKRLADEGLAREAIVALLLGDELGPSWDVAAPKLGQWSALAP
                     GQGLADYLLGRNLYGRGRYHEAALALDRALGRKLPEPRVLDEALRLRLVLACALVDVD
                     AARWALERLRARKLSAARREAVERLAERCSI"
     gene            complement(4963..>5906)
                     /locus_tag="VFQ35_16190"
     CDS             complement(4963..>5906)
                     /locus_tag="VFQ35_16190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET9932245.1"
                     /translation="KDRMELIDVLGYTRTEKRSIAEKFLVPKQLRENGLTAEQVIFPR
                     EGLETIIDHYTREAGVRSLEREISAVCRDVTVRFAEKRLTELVKVTPDVVRELLGPEK
                     VRPELAERKLTPGVALGLGASGGGGELLIVEATRMPGKGEIFITGNMRAVMKESAATA
                     VSFVRSRAERLRLDPEWLRTIDLHLHVPRAAAVRDAAGTGLAMFVAVTSLLLGVSVRP
                     DVAVTGELTLRGSILPVTHLKEQILAAHRAGIRTVLIPARNQRDFADVPEEVKNDIEV
                     HLVSRLDEILPLVLAAPTENESGDRDTDAGSSDEVSP"
BASE COUNT         1020 a         2022 c         1950 g          914 t
ORIGIN      
        1 gagttcgccg agcgcggcga gaccatccca agacatgtca ccggggttga gagcgtgacc
       61 atcgagcacg accaagcgag gcatgcgcgg cgtcttacac ccggcctcgc tcgggtggag
      121 agcaaaacgg agctcccacg aaacagatcc gagcacgagg ccgtgggttc agcgttcctc
      181 aatcgtactg aatccagggc caaggcaagc agtcgcggac agagcccggc gagctagcac
      241 tgggctagcg gcgcgcttcg cttatcgcag cctggctcaa gaaatgttga aacgctgcgc
      301 gctgcgcgct gttcggaacc gaatcgtacg agtacggcct cgagatgcga ttggcgaaca
      361 gacaggtcac cgccttgtcg taagcgcgtg ccgccggcgc gaggtcctcg gcccgcgcgc
      421 tatcgtccgc gatctggcgg acggcggatt ggagctgctc gcaccaccca ggagtctccg
      481 gcaacgtcac cggaggtgac gacgcacagc acgccttgcg catgatcccc gtcgcgggca
      541 attcgtacca accgagcccg gtgccggcgg catcctttac cttgtcagtg acggctgccg
      601 cggtgagcgg ctgctgcttc gcgcgagcga acaccttgtg cgccacctcg cggaagtcac
      661 cgtcttcgca caaaaacgcg aagtcgaggt tcgcctcgat ttgacgctcc gggaagtacg
      721 agcgcacgca gctctcccgc tcttcagcgg ccgagcgctt gggcttctcg ggggcacgag
      781 cgcgcggcgc cgcagccggc gctgcttcta ccgggggcgg agccgcgggg gcatcttgag
      841 tcgtcgcaat ggcgacgtag acggcaagcc cacccgcact cgccgcgagc accacgagcg
      901 gccaaacccg gcgcttgcgc tcgaacgggt tgaagctgag gcgcttcgag attcttgcga
      961 cgacggacgg cggcgacggc gggagcggag ggggcgtcgg cgctcgcggt agctgcgaag
     1021 ccaagtctcc gagtgaaggc gcgtttacgc tgcctcgcac ctcggctggt agtggcggag
     1081 gcatggctcg gtgcggcccc gttccactcg ccgaagcgat cttggggcgc ggcggaggac
     1141 ggaccggctc tgacgcacca aagtcctgag gaagagcccc gacgtcgaac agcacgacat
     1201 cggccgcgac cttggagcca gcgtcgagcc tcgggggcgc ctggcgcgga ggcgaacgac
     1261 gcgctggcat ggtgcgagga tcacgctccc aatcgtcgag cgacttggtg agctccgcta
     1321 gctccgtgac gcgcaggcgt cgctcgccga cgagtccccg cgaaatgatt tcccagagcg
     1381 caggctcgtt gacgccgagt tgaaggagcg cctgcggctt cgtgatcagc gtttgcttta
     1441 ccagcgcgtc gcgctcggtc gcatcgaacg gcaacttgcc ggccagcagt gcgtagagcg
     1501 tcgcgtgcag cgcccaaacg tcgtccgctg cagtcgcggc gccgcctttc aaccgttcgg
     1561 gtgagcaata cgcgccgagc gcaggcgcac tcagctgagt gagaaccggg gcaatggcgc
     1621 gcccctcggg ctcggccacc accgtgaagg gggatatggc accgtgtacg cctccccgtg
     1681 cgtgtaactc acgaacggcg tcgatgagcc tcaggagcca tgcgacggct tttacgggac
     1741 tcactcgctc cgtgagaagt tgctgcagag tgcgccccgg aacgtactcg gcgaccgcga
     1801 tgacgcctcc cggctcgagc acgcttccgc ccggcaattt ggccgcgtcc gcgtcacgaa
     1861 ccaaatcgag cagttcggca agatgcggct gatgcaagcc gcgagcggcg gcgagctcct
     1921 ccgcgcgcgc cttggatacc cacgagagca atacgcgacg gccgctcttg gcctcggtac
     1981 cgagccagcg gtgggcgcca gcttcggact tgagcacggc gtcgaccgag tagcgctcgg
     2041 ctagcaaagt ggattcgtgc atcccgacga ttctctacgc gcgcttggcg cgattgttga
     2101 gagttcaact cgtgagcata gcatcatcgc agtattttct gggaagttct ctctcgcgga
     2161 tcagccgcat aaaaattcct cgtctctcga cctctcacgc tcacgcacat acgcctgcat
     2221 tctctcctcg ggacctctct cgtctcgctc acccgcgaga catcgagttc acctccgccg
     2281 cgcagatccg cgcgcgccat cgatgccaga ggggcgccgc gccgaaacga cccaacgcgc
     2341 tccgcgtgca cgcgacgaaa cggttccttc gaccactccg cgcacgactc gttcgaccac
     2401 gagagccttc gtcggaccgg tcgcttcgaa cgaccgggcc gcccgagcta ggggtgtccc
     2461 aagttccccg ccctgctgcg agccaagatc ctgcggctcg gaagcacggc ctcgtcgctc
     2521 cgctcctcga aaggcttcgg ccttcctccg gtgctcactc ctcgggcgcg cttctcgctc
     2581 gcttttcttg gccctcgcga ggggcaggaa cttgggacaa cccctagttc cgcggcgttc
     2641 agatgctgca gcgctcggcg aggcgctcga cggcctcgcg ccgcgcggcc gagagcttgc
     2701 gagccctgag ccgttcgagc gcccagcgcg cagcgtccac gtccacgagc gcgcaggcga
     2761 gcacgaggcg tagacgcagc gcctcgtcga gcacccgcgg ctctggaagt tttcgcccga
     2821 gcgctcggtc gagcgccagc gcggcttcgt ggtaacgccc acggccgtag aggttccgtc
     2881 cgaggaggta gtcggcgagc ccttgccctg gtgccaacgc cgaccactga ccgagcttcg
     2941 gcgcggcgac gtcccacgaa ggtccgagct cgtcaccgag cagcagcgcg acgatcgctt
     3001 cgcgcgcgag cccttcgtcc gcgagccgct tcacgtcgag cgttcgcagc cgatcttcgt
     3061 cgactagctt ctgagcgagg gcgtcgtaag cgtcgaatgc ctgctcggtt tggccttgcc
     3121 gcaagagcac gtccgcccgc gcttcttgcg cggagagccg cgcccaggga gccatgtcgc
     3181 gagcctcgga gagcttgccg aacgagaccg cggcgcgttg caagtcgttg gcacgcgccg
     3241 cgcatgtcgc aagcccaatg cgtgcgctcg catcgtgcgg atccaagcgg agcacctcgt
     3301 gaaagccttc gcgcgcgccg gtgagatccg aggccgcgag gtgagcgttg gcctccgcgt
     3361 tttctcggtc gatgacgcgc ggacaacgcc gagcgaagaa ggcgggccgc tcgaagcgcg
     3421 ctcgcgcgtt ggcgagcacg gcgtcgctca aagaaatgtg cttcaaggct gcgtgccagc
     3481 gcgcttcgag ctccccgagt gaaacctggg tcacgctctc gagcgtctcg cctccgtacc
     3541 agcgccgcac ggtcgcggca ccgaactcgc cgtgcaagta gcgaacgaag gcgcccgcgg
     3601 ccgtgtacgc ctgcgccgcg ttgtgtccaa agaaggtcag acgaaacaag ctatcgagct
     3661 tgggtaacag ggcgagccgc tgcatcgccg cagcccactc ttcgagcgtg agcgcatcgt
     3721 tctcgtcggg cgaggcggcg gtggcgacac cttcgatgcg gccgggatcc ggcacgagcc
     3781 ccgaaaggag cccggccacc ttgaacggcc ctcgaccgaa gctgcccgcg acgacatgag
     3841 cgagctcatg cccgagcacc gggtgcgggt agcctgccgc ttggatgtag acttcgccac
     3901 gccagggctt cgcgatgtac gtatgggccg cccccatcaa gcgccccttc tcggcgtcgt
     3961 tcgcgaacaa gagcacacgc acgcgctcgg gcccgctcac atcgaagaag cgctccacct
     4021 gagcgaggtg catgtggcac tcgcgaccga tgcgccgcgc gtcgttttcg aggacggcgc
     4081 tcgagtagac gacgtcgcag cgcccggaag tcagccggcg accgagggcg cgctcgatgc
     4141 tgcccgcggt gctgaagtga ccgagctcgg tgccggagac cgtcacgacg aggctcgcga
     4201 cggtggcccc taccccgatc agcgccgcgt tcttgcggtc gaggagggtg cggaagacgc
     4261 cgcgatttcc gcgcgcatcg agcgccacgc tccacgcgag tagagcgatg agcgtggcga
     4321 gcgatccgag acggtagctc gcgagcggcc agagcgacgc gatcaccgtg tcgtagagcg
     4381 gccccgagaa gtagccgaaa taggggtcgt acgcgaatac catcgggctc gtgtaaaacc
     4441 ggaccaggct cagcgcgatc ccggcgagcg gccccccgag cgcaaacagc accgcgacca
     4501 cgcgacgaga gttcggtcga agacgcttcg cgagagctgc ggcgagcgct cccgtagcgc
     4561 atcccatcag cgctcccacg cccggcccca agccatacag cgcgaagccc tctttcgggt
     4621 cacacatgcc gacgcgcaga ccgtggagaa gcgtgagagt cgctgctcct gctaccagca
     4681 cgagcccaaa gtgaagcgca tgcacgaggc tcgccgcaag cgagcgctcc agtcgaaggc
     4741 ttccgagggc tacgctaacg gcggccgtcg aggggaggac gatgccggcg acgagcgcgg
     4801 agtcgtatcc gggtccgccg agctgcggca aaaacccgag cgcagcaagt gctccccacc
     4861 cgaacaaaag ccccacgcgt tcgctgggag ggagccagtc gagaagagcg gcgcgatgaa
     4921 ccaaggaacg acggaggccg ggtccgaagg acaccagccc tcctagggag agacttcgtc
     4981 gctgctcccg gcgtcggtgt cgcgatcgcc cgactcgttt tcggtcggcg ctgccaacac
     5041 gagcggcaag atttcgtcga gccggctcac gagatgcacc tcgatgtcgt tcttcacttc
     5101 ttccggcacg tccgcgaagt cgcgctggtt cctcgcgggg atgaggacgg tgcgaatgcc
     5161 ggcgcggtgc gcggcgagga tctgctcctt caaatgcgtc accggcaaga tgctgccgcg
     5221 cagcgtgagc tcgcccgtga ccgcaacgtc gggacgcacc gagaccccga gcagcagcga
     5281 cgtcaccgca acgaacatcg cgagccctgt gcccgccgcg tcccgcaccg ccgctgcgcg
     5341 cgggacgtga aggtgcaggt cgatggtccg cagccactcg ggatcgagcc gcagccgctc
     5401 ggcgcgcgag cgcacgaacg acaccgccgt cgccgcggac tccttcatca ccgcccgcat
     5461 gttgccggtg atgaagatct cgcctttgcc gggcatgcgc gtcgcttcga cgatgagcag
     5521 ctcgcctccg ccgcccgagg ctccgagccc gagcgcaacg ccgggcgtca gctttcgctc
     5581 ggcgagctcc ggacgaacct tttccggccc gagtagctcc cgcaccacgt cgggcgttac
     5641 cttcacgagc tcggtcagcc gcttctccgc gaagcgtacg gtcacgtcgc gacagactgc
     5701 cgagatctct cgctcgagag agcgaacgcc cgcctcgcgc gtgtaatgat cgatgatggt
     5761 ttcgaggcct tcgcgcggga agatcacctg ctcggcggtg aggccatttt cgcgcagctg
     5821 cttcggcacc aagaacttct cggcgatgga acgcttctcg gtgcgcgtgt agccgagcac
     5881 gtcgatgagc tccatgcgat ctttca
//
LOCUS       DASTKS010000783         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1241222, whole genome shotgun sequence.
ACCESSION   DASTKS010000783 DASTKS010000000
VERSION     DASTKS010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241222"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1664
                     /locus_tag="VFQ35_16195"
     CDS             339..1664
                     /locus_tag="VFQ35_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9932246.1"
                     /translation="MSELKQALLDKISSGTIHVGIIGMGYVGLPLALAFVERAKVKVT
                     GFDVDQSKIDALKAGRTYIMHMGQDRVKQANESGRFTATADFDRLGEPDALMIAVPTP
                     LTPQREPDMQYVVATGEAIAKRLRRGQLVILESTTYPGTTDELLRGILEKTGLKSGTD
                     FFLAFSPEREDPGNKHFNTATIPKVVGGVDPDSGDIAAALYARGLEKVVRVRTARVAE
                     ATKLTENIFRGVNIALVNELKMVYERMGIDVWDVLDAAATKPFGFMKFTPGPGLGGHC
                     IPLDPFYLTWKAREYGINTRFIELAGEVNVNMPRYVIEKLQEGLNNQRKALRGSKVLV
                     LGIAYKKDIDDPRESPSFELIHLLMERGAEVIYHDPHIPTAPAMRSWPDLPPLKSQPL
                     TAELLKSTDAVLISTEHSAVDYDLVLRESPLVIDTRGVYRDPHAKVVKA"
     gene            complement(1803..2792)
                     /locus_tag="VFQ35_16200"
     CDS             complement(1803..2792)
                     /locus_tag="VFQ35_16200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9932247.1"
                     /translation="MNVNVAAAKRTVKTALASNLGWKLFNRTLRRPGVSVLMYHRIRD
                     DDASLGGLPLDVFTAHMEWLRERCDPIWPEELPARLRETRPQKPAVLVTFDDGFRDYH
                     DRAYPVLSRLRIPATVFLATSFMDRGGEGIMWTEELQWAAVSTQKKSVALPWEAQKTI
                     DLPDRNARARLGEIARDHLKSLPDDQRRETLDALLRELGSAPNRGREMLNWDEVRKTT
                     QWTRYGGHTHTHPILSRLPRHEAEREIATCRERIKAETGDDPKTFAYPNGRASDYTAE
                     TQAILKEQGFTLAFTTVEGVALPNSDLLAINRLPAYADNIPDFAYATAGLERV"
BASE COUNT          507 a          969 c          969 g          540 t
ORIGIN      
        1 gacgggcagg aacttgggac acccccctag cctcgggcgc tcgcgacgct aaatgcgttc
       61 agtgctttgt ttgcgctctg cctcggatcg cgcggaggcg atttcgcgac ccggtcctcg
      121 aacctggcag cgccggctcg atggagttcg taaggacgat gcggttcgcg tcgtaagcgg
      181 caattccgct tcggccgatg acgaacgctc ctttcggact tggggcgtcg tggcgctctc
      241 gatccatggg agggtgttga cgtccttcgg aaggtaggct ttcgtccaac gctaggcgtg
      301 gccccaatcg gctccacatc taccgaaatg acaatgtcat gagcgagctc aagcaagcat
      361 tactcgacaa gatctcctct ggaaccatcc atgtcggcat catcggcatg ggttacgtcg
      421 gcctacctct cgcgctcgcc ttcgtcgaac gagccaaggt gaaggtcacc ggattcgacg
      481 tcgatcagag caagatcgac gcgctgaagg ccggccggac ctacatcatg cacatgggtc
      541 aggaccgcgt taagcaggcg aacgagtcgg gtcgcttcac cgctaccgcc gatttcgacc
      601 gcctcggcga gcccgacgcg ctgatgatcg cggtgcccac gccgctcacg ccgcaacgcg
      661 agcccgacat gcaatacgtc gtcgccaccg gcgaagcgat tgcgaagcgt ctgcgccgcg
      721 ggcagctcgt catcctcgag tcgacgacgt atcccggcac caccgacgag cttctgcgag
      781 gcatcctcga gaagacgggg ttgaagagcg gcaccgactt cttcctcgcg ttttcgcccg
      841 agcgcgaaga tcccggtaac aagcacttca acaccgcgac catcccgaag gtcgtcggcg
      901 gcgtcgaccc cgactccggc gacatcgcgg cggcgctcta cgcgcgtggc ctcgaaaagg
      961 tggtgcgcgt gcgcacggcg cgcgtcgccg aggcgacgaa gctcaccgag aacatcttcc
     1021 gcggcgtcaa catcgcgttg gtgaacgagc tcaagatggt ctacgagcgc atgggcatcg
     1081 acgtgtggga cgtgctcgac gcggccgcca cgaagccgtt cggcttcatg aagttcacgc
     1141 ccgggcccgg cctcggcggt cactgcatcc cgctcgatcc cttctacctc acgtggaagg
     1201 cgcgcgagta cggcatcaac acgcgcttca tcgagctcgc gggcgaggtg aacgtgaaca
     1261 tgccgcgcta cgtgatcgag aagctgcaag agggcctcaa caaccagcgc aaggcgctgc
     1321 gcggcagcaa ggtgctcgtg ctcggcatcg cctacaagaa ggacatcgac gacccgcgcg
     1381 agagcccgtc gttcgagctc atccacctcc tcatggagcg cggcgccgag gtcatctatc
     1441 acgacccgca catccccacc gctcccgcga tgcgcagctg gcccgatctc cctccgctca
     1501 agtcgcagcc gctcacggct gagctgctca agtcgaccga cgcggtgctc atctcgaccg
     1561 agcactcggc ggtggactac gacctcgtgc tgcgcgagtc gccactcgtc atcgatacgc
     1621 gcggcgtcta ccgcgatccg cacgccaagg tcgtcaaagc ctgagttcga ccgaggtcgc
     1681 gacgggcgtc acgggcgctc gtcgagaaag cgccgagcac gaaattgctc ggcgcttttc
     1741 tttttgctcg cggggctcgt ggatcgcgcg acccgtgcgc gcggacgaac tcgttcggtt
     1801 ggttaaacgc gctcgagccc ggcggtcgcg taggcaaagt ccgggatgtt gtcggcgtaa
     1861 gcgggcaagc gattgatcgc gagcaagtcg ctgttcggaa gcgcgacgcc ttcgacggtg
     1921 gtgaaggcga gcgtgaaccc ttgctccttc aagatcgcct gcgtctccgc ggtgtagtcc
     1981 gacgcgcggc cgttcgggta ggcgaacgtc ttcgggtcgt caccggtctc ggccttgatc
     2041 cgctcgcggc acgtcgcgat ttctcgctcc gcttcatgac gcggcaagcg cgacaggatc
     2101 gggtgagtgt gggtgtgccc gccgtagcgc gtccactgtg tcgtcttgcg cacctcgtcc
     2161 caattgagca tctcgcggcc gcggttcggc gcgctgccga gctcgcgcag caacgcatcg
     2221 agggtctctc ggcgctggtc gtcggggagt gacttcaagt gatcgcgggc gatttcgccg
     2281 agtcgggccc gcgcattgcg gtcggggaga tcgatggtct tttgcgcttc ccacggaagc
     2341 gcgacgctct ttttctgagt cgaaacggcg gcccactgga gctcttccgt ccacatgatc
     2401 ccttctccac cccgatccat gaacgacgtc gccaagaaca cggtggcagg tatgcggaga
     2461 cggctcaaaa ccgggtaggc gcgatcgtgg tagtcgcgga aaccgtcgtc gaacgtcacg
     2521 agcacggcgg gcttctgcgg acgcgtttca cgcagacgtg cggggagctc ctccggccag
     2581 atcggatcgc agcgctcgcg cagccactcc atgtgcgcgg tgaagacgtc gaggggcaag
     2641 cctccgaggc tcgcgtcgtc gtcgcggatg cggtgataca tcaggacgct cacaccgggg
     2701 cgccggagcg tgcggttgaa gagcttccat ccgaggttcg aggcgagcgc ggtcttgacg
     2761 gtacgttttg cggcggcgac gttcacgttc atggcagcgt tctttcgcta gcttcggcgt
     2821 tttcgcgtgg gcggggccga tcactcctct ttttccggag ccggcggctc tttgcggact
     2881 ttggcgagcg cgcgccgggc cgccgccttc actcgaggga gcacgtggcg gcgcatgcgg
     2941 tacacagcgg ccgcgggggt attccgaaag acatcgacgt aaacg
//
LOCUS       DASTKS010000784         4968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_347954, whole genome shotgun sequence.
ACCESSION   DASTKS010000784 DASTKS010000000
VERSION     DASTKS010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347954"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..2088
                     /locus_tag="VFQ35_16205"
     CDS             325..2088
                     /locus_tag="VFQ35_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932248.1"
                     /translation="MTSSYLQGMEQLVGVVQELSRARDLPRVQEIVRKAARELTGADG
                     ATFVLRDGDRCFYADEDAIEPLWKGQRFPMSACISGWVMLHGKPAVIEDIYADARIPH
                     EAYRPTFVKSLAMIPVRTESPIAAIGNYWATRRLPTDIEMKLLQALANSTSVALENAQ
                     LYAELEERIAQRSAALESARRAEAQALKELEERRRTQAQLDRTEAQLRQSQKMDAVGQ
                     LAGGIAHDFNNLLSVILSFSSMAREALPPGDPIRDDLSEVSLAGERAAALTKRLLTFS
                     RQQIVEPRLVDVGEVTRGMEMMLRRLLGEHVELDVNVAPELFPVMADAGQLEQVLMNL
                     AVNARDAMPDGGKLTIELGNVHLSGEYTNSYLGVSDGDYLMLAVSDTGSGMDRETQSR
                     IFEPFFTTKPQGKGTGLGLSTVYGIVKQSGGHVWVYSEPGEGTTFKVYLPRGSGDAAV
                     PRARPPLVVRLNGSETVLLVEDDEQVRKAARGILRRNGYEVVEATGASQAVEFAESGG
                     RFDVLLTDVIMPQVGGMQLIERLRAVRPELRAVCMSGYAAEAALRHGLIERGIAFVQK
                     PLTPEALLLKVREALEQAPIC"
     gene            2134..3498
                     /locus_tag="VFQ35_16210"
     CDS             2134..3498
                     /locus_tag="VFQ35_16210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9932249.1"
                     /translation="MRLSFDHGTLVLSEAPDRKLDFIPGLSWDPRVALYRAPAFRFVD
                     VASALAQSGLPFVDEVKPRAGGNTGPWNDFELRPYQDAALLAWQLAGRRGTVVMPTGS
                     GKTRVALAALASTGARALCLVPTRALLEQWLRELASARAAPVGCFGDGRRERAPITVA
                     TFESAFRHMHELGADFELLVVDEVHHFGGTSRDAALEMCAAPLRLGLTATPPEPRGLA
                     LLSELVGPVCYQLAIGDLTGKWLACFDRVVLRIPLEPDERERYGRGRAQFSALCSDFY
                     RLHPGASFQDFVSAALKSSEGRAGLRAFRDNQRLVGYTRGKDRALEELLARHRDSKVL
                     IFTADNDAAYTIARRHLIMPITCDTKRRERERVLAAFRGGELRALVSARVLNEGIDVP
                     DADVAIIVGAALGAREYVQRLGRLLRPAPGKRATIYELVSRGTDESRRAARRRRSVAP
                     TQSR"
     gene            3476..4672
                     /locus_tag="VFQ35_16215"
     CDS             3476..4672
                     /locus_tag="VFQ35_16215"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HET9932250.1"
                     /translation="MLPPKVAELCLRVDAHGVRPRYFTERDLPWIAALLGEYRAFEGQ
                     RRSRLRERLREPLSVRAPFGKLRALNHLLEHLARDPVRAVLAPAEARWLVFGSAAQRP
                     APRAEVLARVAAAQNVDAASLEAALFADLDSERRVAPLPSAFEPRDCLPMANRALVAA
                     LLARAVEVRVRVAEDGAARLARAATRLGLICTARPMHPERASDEGKRSEPRLYVSGPL
                     TLFRHSHAYARALAALVSSLVGSFFELDAECRFGRRPTSLRIEASDPLGESELVTEAR
                     CTPFDRFVADFHELSLEWKLEATCMPSTEGALALQDFVLFERERPERRWLLQILPFWT
                     ARSLAAALARATAENGLLCVDGTGACDGTPAPTSEQSASRVLLYRKRLDARKVLAWLS
                     AQAREP"
     gene            complement(4755..>4968)
                     /locus_tag="VFQ35_16220"
     CDS             complement(4755..>4968)
                     /locus_tag="VFQ35_16220"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9932251.1"
                     /translation="AASEGKMKGVLGYTDEKVVSTDFVGEVCTSVFDAEAGIQLDKTF
                     VKVVSWYDNEWGYSNKVIDLVKRIAG"
BASE COUNT          795 a         1618 c         1684 g          871 t
ORIGIN      
        1 ctcgaagagt tcccgccacc agccgcgtga gtcgggaaat tgacgtgcgg cgatgtcctc
       61 gcgtcgtcgc cgcgcgtcag tgcggtcgaa tgcattcgtg cctcacccgc agtgtcagga
      121 attgcgcgac gaacgacgcg ctccctcctg gaaatacaaa ttcaaaacgc atgatttgta
      181 cgggacaagt cgttcgagtt gcgatgagca atcgcattgt tttgtctcgc gcacagtgtg
      241 cgtcgttgtg ccgttcgaga cgttagatca actgtcatat cgcctgcgtg cgacgtgctc
      301 gatgtaacgc tacgttgaag cagagtgaca agttcgtatc tgcaggggat ggagcagctc
      361 gttggcgtcg ttcaagagtt gtcccgagcg cgagacctgc cgcgcgtgca ggagatcgtt
      421 cggaaggcgg cccgcgaact cacgggggcg gacggcgcga cgttcgttct gcgcgatggc
      481 gaccgctgct tttatgctga cgaggacgcg atcgagccgc tttggaaagg gcagcgtttt
      541 ccgatgtcgg cgtgcatcag cggctgggtg atgctgcacg gcaagcccgc cgtgatcgaa
      601 gatatctacg cggacgcgcg gattccgcac gaggcgtatc ggcccacgtt cgtgaagagc
      661 ctcgcgatga tccccgtgcg gaccgaaagc ccgatcgctg cgatcggaaa ctactgggcg
      721 acacggcggc tgccgaccga catcgagatg aagctgctgc aagcgctcgc gaacagcact
      781 tcggtggcgc tcgagaacgc gcagctttac gccgagctcg aggagcgcat cgcgcagcgg
      841 tccgccgcgc tcgagagcgc tcggcgcgcc gaggctcagg ctttgaaaga gctcgaagag
      901 cgacgacgaa cccaggctca gctcgaccgc accgaggcgc agctccgcca gtcgcagaag
      961 atggacgcgg tggggcagct cgcgggcggc atcgcgcacg acttcaacaa cctcttatcg
     1021 gtgatcctca gcttcagcag catggcgcgc gaggcgttgc ccccaggaga tccaatccgc
     1081 gacgatctct cggaagtttc gctcgcgggc gagcgggccg ccgcgctcac caaacgcttg
     1141 ctcacgttca gtcgtcaaca aattgtagag ccgcgtttgg tggacgtcgg ggaggtgacg
     1201 cggggcatgg agatgatgct tcggcgcttg ctcggagagc acgtggaact cgacgtcaat
     1261 gtcgcgcctg agctcttccc ggtcatggcc gacgcggggc agctcgagca ggttctgatg
     1321 aacctcgcgg tgaacgcacg cgacgcgatg ccggacggcg gcaagttgac gatcgagctc
     1381 ggcaacgtcc acctctcggg tgaatatacg aactcgtacc tcggcgtcag cgacggcgac
     1441 tacttgatgc tcgcggtttc ggatacgggc tcgggtatgg accgcgagac gcagtcgcgc
     1501 atcttcgagc cgttcttcac gaccaagccg cagggtaagg ggaccggcct cgggctctcg
     1561 accgtgtacg ggatcgtcaa gcaaagcggc ggtcacgttt gggtctacag cgagcccggc
     1621 gaagggacga cgttcaaggt gtacttgccg cgtggctccg gcgatgcggc ggtgcctcgc
     1681 gcgcggcccc cgctcgtggt gcgcctcaat ggctcggaga ctgtgctctt ggtcgaagac
     1741 gacgagcagg tgcgcaaagc ggcgcgcggc attctccgtc gaaacggcta cgaggtggtg
     1801 gaggctaccg gcgcgagcca ggcggtcgaa tttgccgagt ccggcggccg cttcgacgtg
     1861 ctcctcacgg acgtcatcat gccccaggtc ggagggatgc agctcatcga gcgactgcgc
     1921 gcggtccggc cggagctccg tgcggtgtgc atgtcgggtt acgcggccga agcggccttg
     1981 cgccacggct tgatagagcg cggcatcgcg ttcgtacaaa agccgctcac ccccgaggcg
     2041 ctcctcctca aggtgcgcga ggcgctcgag caagcgccca tctgttagtc gaattttcgc
     2101 aactcgcagg cgtcgaggcc gaccttcgac cgcatgcgac tctcgttcga tcacggtacc
     2161 ctggtccttt ccgaggctcc ggaccgaaag ctcgacttca ttcccgggct ctcgtgggat
     2221 ccccgagtgg cgctgtaccg agcgcctgcg ttcagattcg ttgacgtggc gagcgcgctc
     2281 gcccagagcg ggctgccctt cgtcgacgaa gtgaaacccc gcgccggtgg caacaccggg
     2341 ccgtggaacg acttcgagct caggccctac caggacgcgg cgctgctcgc gtggcagctc
     2401 gcggggcggc gcggaacggt ggtgatgccg actggcagcg ggaagactcg cgtcgcgctc
     2461 gcagcgctgg cgagcacggg ggcgagggca ctttgcctgg tgccgacgcg cgcgttgttg
     2521 gaacaatggc ttcgcgagct cgcttcggcc cgcgcggcgc cggtcgggtg ttttggagac
     2581 ggccgccgag agcgggcgcc gatcacggtc gcgacgttcg aaagcgcgtt ccgccacatg
     2641 cacgagctcg gcgccgattt cgagctactc gtcgtcgacg aggttcacca tttcggagga
     2701 acgtcgcggg acgcagcgct cgagatgtgc gccgcgccgc ttcggctcgg cctcaccgcg
     2761 actccacccg agccgcgcgg cctcgcgctc ttgtccgagc tggtcggtcc cgtttgttac
     2821 cagctcgcga tcggcgatct taccggaaag tggcttgcat gcttcgaccg cgtcgtgctg
     2881 cgcatcccgc tcgagcccga cgaacgcgag cgttacggcc gcgggcgcgc tcagttttca
     2941 gcgctgtgca gtgattttta ccgcttgcac ccgggggcaa gcttccagga tttcgtgagc
     3001 gccgccctga agagcagcga ggggagagcc ggtctgcgcg cgttccgaga caaccagcgc
     3061 ctggtcggtt acacgcgcgg caaagatcgc gccctcgagg agctgctcgc ccgccatcga
     3121 gactccaagg tgctcatctt cacggccgac aacgacgccg cgtacaccat cgcgcgccgc
     3181 cacctcatca tgccgatcac gtgtgacacc aagcgccgcg agcgcgagcg ggttctcgcg
     3241 gcgtttcgag gcggggagct cagggcgctc gtcagtgcgc gcgtcctgaa cgaaggcatc
     3301 gacgtgcccg acgccgacgt cgccatcatc gtcggcgccg cgctcggagc gcgagagtac
     3361 gtgcagcgct tggggcggct actgcgtcca gcgccgggaa agcgcgcgac catctacgag
     3421 ctcgttagcc gcggaaccga cgagtcgagg cgcgcggcga ggaggaggcg ctccgttgct
     3481 cccacccaaa gtcgctgagc tctgtcttcg cgtcgacgcg cacggcgtga ggccgcgcta
     3541 tttcaccgag cgcgatttgc cttggatcgc ggcgttgctc ggcgagtacc gcgccttcga
     3601 agggcagcgt cgttcgcggc tccgcgagcg gctccgcgag ccgctttcgg tgcgcgcgcc
     3661 gttcggcaag ttgcgggcgc tgaaccactt gctcgagcat ctcgcccgcg atcccgtgcg
     3721 agcagtgctc gcgccagccg aagcgcgctg gctcgtgttt ggttccgccg cccagcgacc
     3781 cgccccgcgc gcagaggtgc tcgcccgcgt ggccgccgcg caaaacgtcg acgccgcgtc
     3841 gctcgaggcc gccttgtttg cggacctgga tagcgagcgt cgagtcgcgc cccttccgag
     3901 cgcgttcgaa ccccgcgact gcttgccgat ggcgaaccgc gcgttggtcg cggcgttgct
     3961 cgcgcgcgcg gtagaggtcc gagtgcgcgt tgcggaggac ggcgccgctc ggctggcgcg
     4021 cgctgcgacg cgactcggcc tgatttgcac tgctcgtccg atgcacccag agcgcgcatc
     4081 ggacgaggga aagcgctccg agccccggct ctacgtatcc gggccgctca cgctgtttcg
     4141 ccactcgcac gcctacgccc gcgcccttgc ggcgctggtg tcttcgctgg tcggctcctt
     4201 cttcgagctc gacgccgagt gccggttcgg ccggcgaccg acctcgcttc gaatcgaagc
     4261 gtcagacccg ctcggagaga gcgagctcgt aaccgaagcc cgatgtacgc cgttcgaccg
     4321 cttcgtcgca gattttcatg agctctctct cgagtggaaa ctcgaagcga cgtgtatgcc
     4381 gagcaccgaa ggagcgctcg ccctccaaga tttcgtcctg ttcgagcggg agcgcccgga
     4441 gcgtcgctgg ctcttgcaga ttctgccgtt ttggaccgcg cggtcgcttg cagccgcgct
     4501 cgcgcgggca acggcagaga acgggttgct gtgcgtcgac gggacgggtg cttgcgatgg
     4561 gacgccagcg ccgacgagcg agcaatcggc ttcgcgcgtg ctcctttatc gcaagcggct
     4621 cgatgcgcgt aaagtgctcg cctggctttc ggcgcaggcg cgcgagccct gagcgacggc
     4681 cgaaaaagaa acgaggcggg ccgggacttt gcccgacgcc gcctcgtcgc tgtcgctacc
     4741 gaaaggcgcg actttcagcc cgcgatgcgc ttgaccaggt cgatgacctt gttcgagtag
     4801 ccccactcgt tgtcgtacca ggagaccacc ttgacgaagg tcttgtcgag ctggatgccg
     4861 gcctcggcgt cgaacaccga ggtgcacacc tcgcccacga agtcggtcga gacgaccttc
     4921 tcgtcggtgt agccgaggac gcccttcatc ttgccttcgg aggccgcc
//
LOCUS       DASTKS010000785         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1687808, whole genome shotgun sequence.
ACCESSION   DASTKS010000785 DASTKS010000000
VERSION     DASTKS010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687808"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFQ35_16225"
     CDS             <1..1504
                     /locus_tag="VFQ35_16225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9932252.1"
                     /translation="PSTFFPEVDEGMERVYVRFAPGTPLAEANKKLIDMGRMLKERLP
                     AGSVKLVLTNAGSPGKARSAMNSPNAGPHMGFIRLALSDAEEREQSQRELADQMRALL
                     VHQYPGVDFLQAPGGLVASVFANGYLAPIVIELRGDDLNELMEGSRRIANVARQVPGV
                     RDIYTNLETEYPELRVETRRDEAGMVGVSARDAAQTTLEATLGNINTPGVWVDEGNGQ
                     SYYVVTFYDGAAVSDRAALSSLPVKPSPDAGAVALGTYAEIRRSFGPIAIERNHLGRV
                     ATVYCQTERRDVGSAAEELERRLQSDPSTRDLPFSFVGQVDLMRSTFSGLGVAIGLAI
                     MVVFMIMASQFKSFRLPLVMLFAVPVSLIGIVLALMAAGQGFSVTALMGALMVIGIAV
                     SNGILLVDHANRKLEEGLDVVPAVLDAARRRVIPIAMTSLSTIVGLLPTALGLEKAAA
                     ANQPLALAVVGGLGSSTLLSLFLVPSMFTLLVRKRSAGSDVLGEPAEVLA"
     gene            1584..1976
                     /locus_tag="VFQ35_16230"
     CDS             1584..1976
                     /locus_tag="VFQ35_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932253.1"
                     /translation="MNVSKFAVLAAVIAVCASGCMLKQDGELYTIKTGQKSPIIIEHP
                     IASAGGIHGSLPSGTACDGTFGLVSPEDAKQISNSEVPFSENAGASVAILNCGRNVLR
                     CTLARRPGSGFSYGACKDQQGEEYAMMF"
     gene            2042..2437
                     /locus_tag="VFQ35_16235"
     CDS             2042..2437
                     /locus_tag="VFQ35_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932254.1"
                     /translation="MHKTSLKLISVLTLVATAGCALPRDGYLYSPTQPGRGTLKFADS
                     KADHGELVAALANGERCQGRYATIPGPRVSWDYEQVGVVEMEDTQDGMAVFECQQGHL
                     LRCTFTRELAGAGMGTCLDNHGDKLTMYF"
     gene            complement(2538..>3036)
                     /locus_tag="VFQ35_16240"
     CDS             complement(2538..>3036)
                     /locus_tag="VFQ35_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HET9932255.1"
                     /translation="VRVVRAMPNTAAIALAGATGIAPGSRATEADIIATRSLFDATGR
                     SVVLDESLIDAVTGLSGSGPAYVMLMIEALADGGVKVGLHRDTALLLAAQTVFGSAKL
                     LLDTGEHPGRLKDMVTSPGGTAIAGLHTLESGGLRRTLIDAVEAATRRAQELGEIMAK
                     KLVKA"
BASE COUNT          504 a         1018 c          992 g          522 t
ORIGIN      
        1 gcccagcacc ttcttccccg aagtcgacga aggcatggag cgcgtctacg tccgcttcgc
       61 ccccggcacg cctctcgcgg aggcgaacaa gaagctcatc gacatgggcc gcatgctgaa
      121 agagcggcta ccagccggca gcgtcaagct cgtccttacc aacgcgggct ccccgggcaa
      181 agcgcgcagc gccatgaaca gccccaacgc tggcccgcac atgggcttca ttcgcctcgc
      241 cctctccgac gcagaagagc gcgagcaaag tcagcgagag cttgcggatc agatgcgcgc
      301 gctgctcgtt catcagtatc cgggcgtcga ttttctgcag gcccctggcg ggctcgtcgc
      361 gagcgtgttc gcgaacggct accttgcccc gatcgtcatc gagctgcgtg gcgacgacct
      421 caacgagctc atggagggct cgcgacgcat cgccaacgtg gcccgtcagg tgccgggcgt
      481 gcgcgacatc tacacgaacc tcgagaccga atacccggag ctgcgcgtcg agacgcggcg
      541 tgacgaggcg gggatggtcg gcgtctctgc gcgcgacgcc gcgcagacga cgttggaagc
      601 gaccctcggc aacatcaaca cgcccggcgt ttgggtcgac gaaggcaacg ggcagtcgta
      661 ctacgtggtc acgttctacg acggcgcggc cgtgagcgat cgggcggccc tgtcttcgct
      721 cccggtgaag ccttcgccgg acgccggtgc ggttgcgctc ggaacctacg ccgagattcg
      781 gcgctcgttc ggccccatcg ccatcgagcg aaatcacctc gggcgcgtcg ccaccgtgta
      841 ctgtcaaacc gagcgccgcg acgtcggcag cgccgccgaa gagctcgagc gacgtttgca
      901 gagcgatccg agtacgcgcg atctcccctt ctcgttcgtc gggcaagtcg atttgatgcg
      961 cagcaccttc tcggggctcg gcgtcgcgat cgggctcgcg atcatggtcg tgttcatgat
     1021 catggcgagc cagttcaagt cgtttcgcct gccgctcgtc atgttgttcg cggtgcccgt
     1081 ttcgttgatc ggcatcgtgc tcgcgttgat ggcggcgggg cagggctttt cggtgacggc
     1141 gctgatgggc gcgctgatgg tcatcggcat cgcggtttcg aacggaatcc tcctggtcga
     1201 tcacgcgaac cggaaactcg aggagggcct cgacgtggtg ccggcagtgc tcgacgcggc
     1261 gcgacgtcgc gtcattccga tcgcgatgac gagcctctcg accatcgtcg gcctacttcc
     1321 gaccgcgctc gggctcgaga aggcagccgc ggcgaaccag cccctcgcgc tcgcggtcgt
     1381 gggcgggctc ggttcgtcca cgctgctctc gttgttcctg gtgccctcga tgttcacgct
     1441 gctcgttcgc aagcgaagcg ccggaagcga cgtgctcggg gagcccgccg aggtgctcgc
     1501 ctagccgcgg tcgtcgcgcg gtcttcgttt cgcaaaacta cgtacttcgg tcctttcaga
     1561 accccacaag cggagtcgat cccatgaacg tctcgaaatt cgccgtgctc gccgccgtca
     1621 tcgcggtgtg cgcaagcggt tgcatgctga agcaggacgg tgagctctac accatcaaaa
     1681 ccggccaaaa atcccccatc atcatcgagc atccgatcgc tagcgccggt ggcattcacg
     1741 gttcgctccc gagcggcacc gcttgcgacg gaaccttcgg cctcgtgtcg cccgaagacg
     1801 cgaagcaaat ctcgaactcg gaggtgccct tcagcgagaa cgccggcgcg agtgtcgcca
     1861 tcttgaactg cggccgcaac gtgttgcgtt gcacgttggc gaggcggccc ggtagcggct
     1921 tcagctacgg cgcatgcaaa gatcagcagg gcgaagagta cgcgatgatg ttctgagtgc
     1981 cgacgcgcct cacgagctct cgttccgcct ggtggtcgcc ctctgacccg gagtccaacc
     2041 catgcataaa acctcactca agctcatctc ggtcctgacg ctcgtagcta ccgcaggctg
     2101 cgcattgccc cgcgatggct acctctattc accgacgcag cccggacgcg gaacgctgaa
     2161 gttcgcggac tcgaaggccg accatggcga actcgtcgcg gcgctcgcca acggggagag
     2221 gtgccaggga agatacgcga ccattcccgg gccgcgcgtg agctgggact acgagcaagt
     2281 cggcgtggtc gagatggaag acacccaaga cgggatggcc gtgttcgagt gccagcaagg
     2341 ccacctgctg cgctgcacgt tcacgcgcga gctcgccggc gcgggcatgg gcacttgtct
     2401 cgacaaccac ggtgacaagc tgaccatgta cttttgacgg gcgagcccga gggattcctc
     2461 cggggggccg ctcatgcgac ggcgcccgtt gctctggcgc gtgaacacgg cgtgcggtgc
     2521 ccgcgcgccg ggcgcactca cgccttgacg agtttcttgg ccatgatttc gccgagctct
     2581 tgcgcgcgtc gcgtcgcggc ttcgaccgcg tcgatcagcg tgcgccgcaa gccgccggat
     2641 tccagcgtgt gcaagcccgc gatcgcggtg ccaccggggc tcgtcaccat gtccttcaag
     2701 cgcccggggt gctcaccggt gtcgagcagg agcttcgcgg agccgaacac cgtttgtgcg
     2761 gcgagcaaga gcgccgtgtc acgatgcaag ccgaccttaa cgccgccgtc ggcgagcgcc
     2821 tcgatcatca acatgacgta agctggtccg ctgccggaga gccccgtcac ggcgtcgatc
     2881 aagctctcgt cgagcacgac cgagcgcccg gtggcgtcga acagcgagcg cgtcgcgatg
     2941 atgtcggcct cggtggcgcg cgaacccggc gcgatgccgg tggcgccggc gagggcaatc
     3001 gccgcggtgt tcggcatcgc acgcacgacg cgaacg
//
LOCUS       DASTKS010000786         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1241811, whole genome shotgun sequence.
ACCESSION   DASTKS010000786 DASTKS010000000
VERSION     DASTKS010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241811"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFQ35_16245"
     CDS             <1..545
                     /locus_tag="VFQ35_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET9932256.1"
                     /translation="KKSGDAKMGDLVALDDNNFLTIEAGKDKDGKLRNVVYRFDISGA
                     TDLTDLTLPSGTNAGKALEYGTPAELSSVITMASKQLVLDLRAYGYTAEKSEGMALVD
                     DRTLVVINDNDFGATAVIENDANTTDPTKYVVDSTGALSFNGAPSPGSYAIHALEPEA
                     QATNLFVVHLQEPFAGYCSH"
     gene            complement(662..>2099)
                     /locus_tag="VFQ35_16250"
     CDS             complement(662..>2099)
                     /locus_tag="VFQ35_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932257.1"
                     /translation="PGEGRLPRDKDVYERVVATFNGAFKTTHGAYGMMVQRRVLLPPV
                     KGGATVVVNDSHEIGLGSWPQSEEIPQSVISFRQNLDPLVEDGVANPTGRQLWGWQLD
                     GTSVMTQRTALCVTPAGHLYYAFGAEIDGKTLGKAMYQAGCAYAMHLDMNPAHCGFVF
                     SDVRNPRSGDMTLRLLDDEMKIAPDKYARWSAKDFFYVSVRDATPHDASGVLWTADGG
                     VQPPPAWLPGVFSGKLTLGSLEVNLLSIEKGRVEYRVRAGSREPGRKKRTGHGLSDED
                     ATRVLAALGLGHATEATRYGMTYAGDELSSLKANYATVVFEDGFPPRVVLPNGAAPTL
                     TEKQEAVQLPLLAADAKLLDRARDRGDRRQRAAFCVTPSGRVIVGSAEHDSSDGIASA
                     LMRVGCREVLELDRGSHHPAYVHRAGGSTPPMDEYEASTLYVLGRPMIPHAFRWKADG
                     SVPSKRVTSYDVPHPEDVPPPRRKRASK"
BASE COUNT          362 a          684 c          680 g          373 t
ORIGIN      
        1 acaagaagag tggcgacgca aagatgggcg atctcgtcgc gctcgacgat aacaacttcc
       61 tcaccatcga agccggcaaa gacaaagacg gcaagctccg caacgtcgtc tatcgattcg
      121 atatcagcgg cgcgaccgac ctcaccgacc tcactctgcc gagcgggacg aacgccggta
      181 aagctctcga gtacggcaca ccggccgagc tatcgagcgt catcaccatg gcgagcaagc
      241 agctagtcct cgacttgcgc gcctacggct acaccgccga gaagtccgaa ggtatggcgc
      301 tggtcgacga tcgcacgctc gtggtcatca atgacaacga tttcggcgcc accgccgtca
      361 tcgagaacga cgcgaacacc accgatccga cgaagtacgt cgtggattcg accggggcgc
      421 tctcgttcaa cggcgcaccg tctcccggaa gctacgcaat ccacgcgctc gagcccgagg
      481 cccaagccac gaacttgttc gtcgttcacc tgcaggagcc cttcgctggg tactgcagtc
      541 actgaagcga gtcctaacgt tgagagccag tcctatcggg gggaggatag gacttggctc
      601 ttggtcgcga ctcggatcat cagcactcga tcagctcgcc cgctcggttt gcgccgctcg
      661 gtcacttgct ggcgcgcttg cgcctcggag gcggaacgtc ctcggggtgc ggcacgtcgt
      721 agctcgtcac gcgtttgcta ggcacgctgc cgtctgcctt ccagcggaag gcgtgcggaa
      781 tcatgggtcg gccgagcaca taaagggtgc tcgcttcgta ctcgtccatc gggggcgtgg
      841 agccaccggc gcgatgcacg taagcgggat ggtgagagcc gcgatcgagc tcgagcacct
      901 cgcggcaacc gacgcgcatg agggcgctcg cgatcccgtc gctgctgtcg tgctcggcgc
      961 ttccgacgat gacgcggccg ctcggcgtca cacagaaggc ggcccgctgt cggcgatcgc
     1021 cgcgatcgcg agcgcgatcg agcagtttag cgtcggccgc cagcagcggg agctgcaccg
     1081 cttcttgttt ttcggtgagg gtgggcgcgg cgccattcgg aaggacgacg cgcggcggga
     1141 agccatcctc gaagacgacg gtcgcgtagt tcgctttcag tgacgagagc tcgtcgccgg
     1201 cgtaggtcat tccgtaccgc gtcgcctcgg tggcgtgccc gaggccgagc gcggcgagca
     1261 ctcgagtcgc gtcctcgtcg ctcaatccgt ggccggttcg ctttttgcgt cccggttctc
     1321 tcgagcccgc gcgaacgcgg tactccacgc ggcctttttc gatgctgagc agattcactt
     1381 ccaggctgcc gagcgtcagc tttccgctga acacgccggg gagccacgct gggggcggct
     1441 gcacgcctcc gtcggcggtc cacagcacgc cgctcgcgtc gtgtggcgtc gcgtcgcgca
     1501 cggagacgta aaagaagtcc ttcgccgacc agcgcgcgta cttgtcgggc gcgatcttca
     1561 tctcgtcgtc gagcaagcgc agcgtcatgt caccgctgcg gggattgcga acgtcggaga
     1621 aaacgaagcc gcagtgggcc gggttcatgt cgaggtgcat cgcgtaggcg cagccggctt
     1681 ggtacatggc tttgccgagc gtcttgccgt cgatttcggc gccaaaagcg taatagaggt
     1741 gccccgcggg agtgacgcaa agggccgtgc gctgggtcat cacgctcgtc ccgtcgagct
     1801 gccaacccca gagctggcgc ccagtggggt ttgccacgcc gtcttcgacg agcggatcca
     1861 aattctggcg gaacgagatc acgctctgag ggatctcctc gctctgcggc cagctcccga
     1921 gcccgatctc gtgcgagtcg ttgacgacca ccgtcgcccc gcccttgacc ggagggagca
     1981 acacgcggcg ttgcaccatc atgccgtagg cgccgtgcgt ggtcttgaag gcgccgttga
     2041 aagtcgcgac cacgcgctcg tagacatcct tgtcgcgcgg taaacgacct tctccgggt
//
LOCUS       DASTKS010000787         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_794360, whole genome shotgun sequence.
ACCESSION   DASTKS010000787 DASTKS010000000
VERSION     DASTKS010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794360"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFQ35_16255"
     CDS             complement(<1..551)
                     /locus_tag="VFQ35_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932258.1"
                     /translation="MSTRRLQLFVLAAMSVLAGCSAGSGTEVDTGGRGNAQGGHDSSG
                     GTQSGGTLGEGGRTTTPTGGASSAMGGASFGGANGSGGSSSGGSANSSGGQSGGRGGA
                     TSGGQSSGAGRGGTTNSGGTSAGGRGGASASGGASSSGGASSTAGSGPITPGKKFVGN
                     ITTGNSIDSGSLVFAKYWDQITP"
     gene            complement(788..1843)
                     /locus_tag="VFQ35_16260"
     CDS             complement(788..1843)
                     /locus_tag="VFQ35_16260"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET9932259.1"
                     /translation="MRPLLAFSIALAALLSGALAFAHAVGLSNGEYERTPGGLRAEMV
                     FAHSDLIAALPELDRNRDGSVDGTELSESRARLHQFAAEALVVRAGSERCRLLETLPR
                     LTDAMGLSLSSSFECPRVDAALSIELRFLNELGREHRHRAKVVGGAERLVFGGDARLE
                     LPALGSASDAHSSYTEYVRLGFGHILSGLDHIAFLLALLLSVRRIKTLLAVVSAFTLA
                     HSLSLALVTLGAWAPSPRYVEPAIALSIVYVALEGATGRMPTRPALVSFAFGLVHGMG
                     FAGALGELNLTPSEIPVALVLFNAGVELGQLLLLFALVPSLRWLTKYGWFQRRVLPLC
                     SVALAVCGVFWFAARVG"
     gene            complement(1845..3170)
                     /locus_tag="VFQ35_16265"
     CDS             complement(1845..3170)
                     /locus_tag="VFQ35_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932260.1"
                     /translation="MQRVRPWTIALILAATACQRSPRELARAPRDHAAAKAAESEPER
                     ARSLALSGSTGGARIDAEIRALITRARKQPQKAATWIELGRSYVDKARAASDPGYYLN
                     ADACAEVALGISPDDPLALDLRALVLLNNHEFTKAKALSESLIARRPDAAMAWGSLSD
                     ALLELGEQDAAERAANTMLDLKPNLPSYSRASYLRWLRGAESDARELARLAIDASDDP
                     RRPEPRAWMLVQAALLFWHAGDWQGADAGFVRALEVVKDYPPALVGRGRVALADGNAG
                     AAADFFQRAYDASPLTETAWLLGEARELEGRHAEAKSAFADAEREGRRADRRTLSLMF
                     STQARNIEEAVSLAERERQVRADIYTEDALAFALYRAGRMHEANEAIQRARRLGTPDA
                     KLMFHDGLIRLGLGDSRSGKRLLSEALAQNAHFDERSAREARTLLGTGG"
     gene            3410..4444
                     /locus_tag="VFQ35_16270"
     CDS             3410..4444
                     /locus_tag="VFQ35_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932261.1"
                     /translation="MRTIVVRPGPFRPVFRIGCFLVAALGFTLGCADTKSDSSGTNPT
                     NEGPFKSLEAISNAPSTFSSPRAGAPLSDGSVVLIATLESAPTEDATDSGERVAILKQ
                     SPGGAPPSVLYSGDALVNPLDLAVSLDGETVYVADPSGGPDGSGAVSTLSSSGGEPSV
                     VLAGYRPRAITVADDGRVYVSGVSNESGAPGVFELRGGTAVSVFEGAPLVDPSGIAVQ
                     ADGTVLVADTRLFDASADVSSEAGIVRIQGGKASVFASGFATGYPAGIALTRDEKTLI
                     VSGEGPDRSDTVFLVDMSAPAQPPVAVTADFSRYQDASAGLKRAHDSNTFIWASLAAN
                     GGTVFRIVAN"
     gene            4483..>5185
                     /locus_tag="VFQ35_16275"
     CDS             4483..>5185
                     /locus_tag="VFQ35_16275"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HET9932262.1"
                     /translation="MMTIRRASTVLASLFTLLAAAQANASSHREAPAISADPAADNTD
                     LYAWACNEAGTPSLCIAANYIPLELPEGGPNYHKFSDDVLYQLHVTYNDRNGVPTLTD
                     YMTIDVRFETKTRSRVAVDDKKAGPGGGKEFFAQIGGEFDQTADVVLRRADGRAERIF
                     QGVDVAPPNIGQRTATFAYGNPSYADYAQKFVVNGDRGFRFFAGPRDDAFYVNLAQIF
                     DLGAFVGLNTLNGQPF"
BASE COUNT          885 a         1730 c         1661 g          909 t
ORIGIN      
        1 tcgggagtga tctggtccca atacttggcg aacacgaggc tccccgagtc gatcgagttg
       61 ccggtcgtga tgttgccgac gaacttcttt ccgggagtga tgggcccgct accggcggtc
      121 gagctcgcgc cgccgctcga gctcgcgcca ccgctcgcac tagcgccgcc tcggccgccg
      181 gcgctagttc ctcccgagtt cgtggtgccg ccacggccgg cgccgctgct ctggccaccg
      241 ctcgtcgcgc caccacgtcc gcccgactgg ccaccagagg agttcgcgct gcctccgctc
      301 gagctgcctc cggagccgtt cgcgccgcca aagctcgctc cacccatcgc agagctcgct
      361 ccacccgttg gggtggtcgt tcggccacct tcgcccaacg tgccgcctga ctgagtgcca
      421 cccgagctgt cgtgcccacc ctgcgcgttt ccgcggccac ccgtatcgac ctcggtgcct
      481 gagccggcgc tgcagccagc caggacggac atagcagcca gaacgaacaa ctgaagccgt
      541 cgcgtactca cgaggagcct cctgcgcaga tctctgcgtg tgacttgtca gtatgccctc
      601 gtatcgagat tttgatctgc gctcaacgat tcttcgcgtg cgtttggcag aactcagcgt
      661 cgagctcctc acccaatagg aattttctag tttgaaggtt tgcgtcggcg cgcacgagct
      721 cgagagttcc ttcaggaaag gaatttgtta atcctctttt ccgaagcgag ctggagcgcg
      781 gccggtccta gccgacgcgc gctgcgaacc agaagacacc gcacaccgcg agcgcaaccg
      841 aacagagcgg caacactcgg cgctggaacc agccgtactt cgtcaaccaa cgcagcgacg
      901 gtaccagggc gaagagcagc agcagctgac cgagctcgac ccccgcgttg aatagaacga
      961 gcgcaaccgg gatctcgctc ggcgtgaggt tcagctcgcc gagtgctccg gcgaagccca
     1021 tgccatgaac cagcccgaac gcgaagctca cgagcgctgg acgggtgggc attcgcccgg
     1081 tcgcgccctc gagcgcgacg taaacgatcg aaagcgcgat ggcgggctcc acgtagcggg
     1141 ggctcggtgc ccacgccccc aacgttacga gcgcgaggct cagagaatgc gcgagagtga
     1201 aggcgctcac cacggcgagc agcgtcttga tgcgcctgac cgagagcagg agcgccaaca
     1261 aaaaggcgat gtggtcgagc ccgctcagga tgtgaccgaa tcccaggcgc acgtactcgg
     1321 tgtagctcga gtgagcgtcg gaagcgcttc ctaacgctgg cagctcgagt cgagcgtcgc
     1381 cgccgaaaac gaggcgttcc gctccgccta ccacctttgc tcgatgtcga tgctcgcggc
     1441 cgagctcgtt cagaaaccga agctcgatcg aaagcgcggc gtcgacgcgc ggacactcga
     1501 acgacgagct caaagagagc cccatcgcat cggtgaggcg cggaagggtc tctagcaacc
     1561 ggcatcgctc cgaacctgct cgaacgacga gcgcttctgc cgcgaattgg tggaggcgtg
     1621 ctctgctctc gctcagctcg gtgccgtcca cggatccgtc gcggtttcgg tcgagctcgg
     1681 ggagcgcggc gatgagatcc gaatgggcga acaccatctc ggcgcgaagc ccgccaggag
     1741 tccgctcgta ctctccgttc gacagcccga cggcgtgcgc gaaggcaaga gcgccgctga
     1801 gaagcgcggc caacgcaatc gaaaacgcga ggagcggtcg cacttcagcc acccgtgccg
     1861 aggagtgttc gcgcttcgcg cgcgctgcgc tcgtcgaagt gggcgttctg ggcgagcgct
     1921 tcggagagca atcgcttgcc gcttcgcgaa tcgccgagcc caagccggat gagcccgtcg
     1981 tggaacatca acttcgcgtc gggagtgccg aggcgtcgtg cgcgctggat tgcttcgttc
     2041 gcttcgtgca tgcgcccggc gcggtacagc gcgaacgcga gagcgtcttc ggtgtagatg
     2101 tcggcccgta cctgtcgctc tcgctctgcg agcgagacgg cctcttcgat gttgcgtgct
     2161 tgtgtcgaga acatcaatga cagcgtgcgc cgatccgcgc gacggccctc acgctccgcg
     2221 tcggcgaatg cggatttcgc ctcggcatgg cgcccctcga gctcacgggc ctcgcctagg
     2281 agccacgccg tctcggtgag ggggctcgcg tcgtacgcgc gctgaaagaa gtcggcggcg
     2341 gcgcctgcat tgccgtccgc caaagcgaca cggccgcggc ccacgagcgc cgggggatag
     2401 tccttcacga cttcgagcgc tctcacgaag cctgcgtccg cgccttgcca atcgcctgca
     2461 tgccagaata gcagcgccgc ctgcaccagc atccaggcgc gcggctcggg cctgcgcgga
     2521 tcgtcgctcg cgtcgatcgc gagccgagca agttcacgcg cgtcggattc ggctcctcgc
     2581 agccagcgca aatacgaggc tcggctgtag gatggcaggt tcggtttgag gtccagcatc
     2641 gtgtttgcgg ctcgctcggc ggcgtcctgc tctccgagct cgagcaacgc atcgctcagg
     2701 ctcccccacg ccatcgccgc gtccgggcgc ctcgcgatca gcgattcgga gagggccttg
     2761 gctttcgtga actcgtgatt gttcaggagc acgagcgcgc gcagatcgag ggcgaggggg
     2821 tcatcggggg aaattcccag cgcgacctcg gcgcaagcgt ccgcgttcag gtagtacccc
     2881 gggtcactcg cggctcgcgc cttatcgacg taggaacgtc cgagctcgat ccaggtcgcg
     2941 gccttttgcg gctgcttgcg tgccctcgtt atcagcgcac gaatttccgc gtcgatgcga
     3001 gccccgcccg tcgagcccga aagcgcgagg gagcgcgctc gctccggctc gctctctgct
     3061 gcttttgctg cggcgtgatc tcgaggcgca cgcgctagtt cgcgaggcga acgctgacac
     3121 gccgtcgcgg cgaggatcag ggcgatggtc cacggacgta ctcgttgcat cgatgcatct
     3181 cctccgagcc cttgcggtcc aagctttgcc cgccgcgcgc cgagatcccc cggctgcaaa
     3241 gcccgctttc tcggcgctcc cgacgggcag ttacttggga cacccgctaa tacgcgcgac
     3301 ctcggtggtt cggatttgcg caggcacgaa atcgacgccg atgtgcgttt ttgcagggtc
     3361 gaacaaatcc ggagaggccg cccgctgcgt aagggggcga ggaggttaca tgcgaaccat
     3421 cgtcgttcgg ccgggaccgt tccggcctgt ttttagaatc gggtgctttc tcgtcgctgc
     3481 cctcggattc acgctcggtt gcgccgacac caaatccgac tcgagcggga cgaaccctac
     3541 gaatgaaggt cctttcaagt cgctcgaagc gatctccaat gcgccgagca ccttttcgtc
     3601 gccgcgcgcg ggggcaccgc tttcggacgg aagcgtcgtc ctcatcgcga ccctcgaaag
     3661 cgcgccgacc gaggatgcaa cggattcggg agagcgcgtc gcgatcctga aacagagccc
     3721 tggcggcgct ccgccgtcgg tgctctacag tggtgacgcg ctggtcaatc cgctcgatct
     3781 agcggtgtcg ctcgacggag agacggtcta tgtcgccgat ccatcgggtg gacccgacgg
     3841 aagcggagcc gtttcgacgc tctcgagcag cggtggagaa ccatccgtgg tcctcgccgg
     3901 ataccgccct cgtgcgatca cagtcgctga cgatggtcgc gtttacgtga gcggcgtcag
     3961 caacgaatct ggtgctccgg gcgtcttcga gctgcgaggc ggtacggcgg tcagcgtgtt
     4021 cgaaggcgcg ccgctcgtcg atccatccgg catcgccgtg caggcggacg gaaccgtgct
     4081 cgtggctgat acgcggttgt tcgacgcttc cgccgacgtc agcagcgagg ccggtatcgt
     4141 gcggatccaa ggcgggaagg cgtcggtctt cgcctccggg ttcgcgaccg gttaccccgc
     4201 gggcatcgcg ctcactcgcg acgagaagac gctgatcgtc tcgggagagg gacctgatcg
     4261 cagcgacacc gtgttcctcg tcgacatgag cgctcccgct cagccgcccg tcgccgtgac
     4321 ggccgacttt tcacgatatc aagacgcttc ggcgggcctc aagcgagccc acgacagcaa
     4381 caccttcatc tgggcttcgc tcgccgcgaa cgggggaacc gtgtttcgca tcgtggcgaa
     4441 ctgagcgtgt ctttcaaggg aagtatcagg agattttaga aaatgatgac cattcgtcgt
     4501 gcttcgacag ttcttgcaag tctgttcacg ctgctcgccg cggcgcaggc gaacgcctcc
     4561 agccaccgcg aggcgcccgc aatttcggcc gaccccgctg ccgacaacac cgacctttac
     4621 gcctgggctt gcaacgaagc cgggactccg tcgctgtgca tcgccgccaa ctacattccg
     4681 ctcgagctcc ccgagggcgg gcccaactac cacaagttct ccgacgacgt cctctaccag
     4741 ctccacgtca cgtacaacga tcgcaacggc gtgcccacgc tgaccgacta catgacgatc
     4801 gacgtgcgct tcgagacgaa gacgcgctcg cgcgtggcgg tcgacgacaa gaaggcgggg
     4861 ccgggtggag gcaaagagtt tttcgcgcag atcgggggcg aattcgacca aaccgcggac
     4921 gtcgtcttgc gacgcgcgga cggccgagcc gagcgcattt tccagggggt cgatgtggcg
     4981 cctccgaaca tcggccagcg aacggcgacc tttgcgtacg gcaacccgag ctacgcggac
     5041 tatgcgcaga agttcgtggt gaacggcgat cgcgggttcc gcttcttcgc cgggccgcga
     5101 gacgacgcct tttacgtgaa cctcgcccag attttcgatt tgggcgcgtt cgtcggcttg
     5161 aacaccctca acgggcaacc ctttc
//
LOCUS       DASTKS010000788         6426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1241898, whole genome shotgun sequence.
ACCESSION   DASTKS010000788 DASTKS010000000
VERSION     DASTKS010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241898"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFQ35_16280"
     CDS             complement(<1..363)
                     /locus_tag="VFQ35_16280"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9932263.1"
                     /translation="MSRVDDELAEAVAQSEASSSTEDAVPVTRPAPVPVKSTKRNLGL
                     LAALLVMGGGILTLVMTSFENAAVYSRGVDSLLKEKDKLVGRNVRIEGALVKGSLMRR
                     DQPCEYRFNLAKGGATVPV"
     gene            complement(512..1174)
                     /locus_tag="VFQ35_16285"
     CDS             complement(512..1174)
                     /locus_tag="VFQ35_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932264.1"
                     /translation="MTVLLAALATVLVYAWRDRAARVERTRFERTLNVAVVIVEAAPV
                     AANALTALRGRTRALEDRLASEFHRYRPDGPRPFGLVVFGPVSQVLPPPSHLEPSALG
                     LAKRAWELLRFTRDVDARAHVPTRGFDTRLYMVVRPPANASRAFVEGASEQGGRVGIA
                     LVELDDTMADFALFVGAHELFHTLGATDKVRHRRPYALSRRARGTRPRAPVAAAVRRF
                     DG"
     gene            1605..1868
                     /locus_tag="VFQ35_16290"
     CDS             1605..1868
                     /locus_tag="VFQ35_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932265.1"
                     /translation="MDKSSWRSEVARLLDHCAAALRAGHDRQALISFGTGIAYLVKAS
                     AAGSLVAGELLAQLNEVAGDGATQRFTFAPGELEELRSRPINE"
     gene            2060..2653
                     /locus_tag="VFQ35_16295"
     CDS             2060..2653
                     /locus_tag="VFQ35_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932266.1"
                     /translation="MATSSAVLVDTGEFEAVTEAELVPDAPEAAASASETHRAIGKVA
                     SEPARPSGQSGIRPSPAALAVRIEAALRASTKTEATLADLLRTAKFLSASIDAVREAN
                     TAVLRELEVLCELVDGEGAERSSLERRIQRLEHLVDETGRDAARDRRFLVEEHDAFIA
                     TLVSDHERELQALRERLAELEQQPAPSSEHEEAGDRS"
     gene            2775..5102
                     /locus_tag="VFQ35_16300"
     CDS             2775..5102
                     /locus_tag="VFQ35_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9932267.1"
                     /translation="MRAAAGPVRKWMLTLVMLWVTALMTTFALPSRAEGNLLSGKSPS
                     RVQGVSNAKVLTDGARAHEGDEWNSIAAAIIESDRAFVVFDLGKVTPIVAAYVQGDNN
                     DEYTLSVSDDDKTFTPLWSAGPRSEPGLRERYNDSLTGQGRYVRLTARGGDRAYSVTE
                     LQIFSEKPKTPPALGRVSGESQASHVRTSLLYVAAAFALWLFTTWASASTVAMLIGAA
                     AVLVAGGIAYEAVDSAWPLANREVAYVRAVSAAIALLAVLRRVAAAKRFPAHRHVVTL
                     ALLASAVMAFASFYNLGRPQFWNHKENRPEFVHTYDMRVYQPFARFFKELQYDGVYLA
                     SVLAFAEDQRHGSLESMANVEVRSLRDHKVQRVGDIGPQIQELRKRFSDDRWAELKKD
                     MLYFEDVMGPEFLGTLTDHGANATPVWVFFARLMLGHAPASEKLLTWAGLADGALLLG
                     MAFAMWRSFGLWPALLAMTVFGANDLYMFGTNWSGATLRHDWLALLGFGACALKSERW
                     VVAGVLLALAAMIRAFPAAALLGVSIPAIWWLYEYWQAERKIPNYRDVYEAHPETVRV
                     LLAAIGTMVFAFLFTGLLYGFHAWGNWWEKVTLLNRDVGVNEISLRALVAGADGNAGR
                     VLQARRAIHIAAVLLSVGTVFALGRGRPLHQAMLIGMPLALVIWNPSNYYSHFVFLLV
                     LLGSVTVPRKSKVTEKSEDDEQEEEPKFQPIWAQLHLTAAPLLVMCIAGYWSALEPDL
                     ERHFQNETVLLFAAFAWVYANLLKRDPKLKSALMN"
     gene            complement(5092..5559)
                     /locus_tag="VFQ35_16305"
     CDS             complement(5092..5559)
                     /locus_tag="VFQ35_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932268.1"
                     /translation="MARRLPVIQSPPSDEAAALSRSSPQWLLIGAAFTVVTFLPLSLV
                     GLWAGSRLARGLARSETAALGWAALPVLLAYVVAAAGAGAVVGRFGQRAHRAVPAGAG
                     ALGGALLVGFSLLKGVGSSAMAVGAACIFIGGGALFAALGASYGRKRRPTLSS"
     gene            5625..6329
                     /locus_tag="VFQ35_16310"
     CDS             5625..6329
                     /locus_tag="VFQ35_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932269.1"
                     /translation="MSLSLVLALPLALAPATLGHIGERPPALAELPAPAAPVDEPPEL
                     PRRTFELWPAAGLSLPQCGVSSGTGCAALSAGPELGLVGLMRPLPHFAFGASARRATF
                     SAKPAEDTWRTSATMSLFSVVGRVYAFEGGRFDPYLELDLGAGSLGVEVTGHGAHKEE
                     ALFSPAVRSVLGVDVVLSGWLRAGAFLAYARYFPGTVSFCSDLGCGAVAASNSGLTLG
                     SLSLGVEVGLVGGETL"
BASE COUNT         1073 a         2092 c         2187 g         1074 t
ORIGIN      
        1 aacgggcacg gtcgctccgc ccttcgccag gttgaagcgg tattcgcaag gttggtcgcg
       61 acgcatgagc gaccccttca cgagcgcgcc ctcgatgcgg acgttgcggc cgacgagctt
      121 gtccttctcc ttcaacaggg agtccacgcc gcgcgagtac acggcggcgt tctcgaagct
      181 cgtcatcacc agggtcagga tgccgccgcc catgacgagc aacgcagcaa ggagacccaa
      241 gttgcgcttc gtcgacttga cggggaccgg tgcgggccgc gtgacgggta cggcatcttc
      301 ggtcgaggac gatgcctcgg attgggctac ggcttcggca agctcgtcat cgacgcggct
      361 catgggggaa aaggtagcct ccagacccca ccggatccac cccggctacg gttttcgctg
      421 ccacccgact tctttggcgg tagcgggacc tacccccagc tcggcgaggg aggtgggcgg
      481 ctcctcgtgg gtgaggtcga gtggtcggtt ctcagccatc aaatcggcga accgctgcgg
      541 caactggggc acgaggtcgg gttccgcgag cccttcggga aagcgcgtac ggcctgcggt
      601 gtcgtacttt gtcggtcgcg ccaagcgtat gaaaaagctc atgtgctccg acgaacagcg
      661 caaaatcggc catggtgtcg tcgagctcga ccagggcgat accaacgcgc ccgccctgct
      721 cgctggctcc ctcgacgaac gcgcgcgatg cgttggccgg cgggcgcacc accatataga
      781 gccgcgtgtc gaagccccgc gtcgggacgt gagcgcgcgc gtcgacgtcc cgcgtgaagc
      841 gaaggagctc ccaagcgcgc ttcgcgaggc cgagcgcgga tggttcgagg tgcgagggtg
      901 gcggcagcac ctgcgacacc ggcccgaata cgacgagccc gaagggacgc ggaccatcgg
      961 gacggtaccg atgaaactca cttgccaatc gatcttcgag cgcgcgggtg cgcccacgca
     1021 gcgccgtgag cgcgttcgcc gcgacgggcg ccgcttcgac gatgaccacg gccacgttga
     1081 gcgtccgctc gaatcgggtg cgctctacgc gcgcggcgcg gtcacgccag gcgtacacga
     1141 gcaccgtcgc gagcgcggcc aagagcaccg tcactcgaat tcgaaatgcg agaccacgcc
     1201 cgcgcacacg cataccaccg atactgaacg ccctcggacc cgtttccaat agaaccgatg
     1261 agggccgcga atgggttcgt cagtcgcgac cgtgccgaga ccgcagcgaa gtgggcgcct
     1321 gacgagtgag cacccgcgaa tgggttcgtc agtcgcgagc ctgcccgagg ccgcagcgag
     1381 gcgagcgcgt agcatctccg ctgacccatt tcgcgacgcg cgaccaagga gggattcgta
     1441 ggcgacgctg cgggccatcg tgagcacgtg gttccggtgt gcgcgcgcgg agtagctcga
     1501 gagcttctcg cgtacgtcgt gcttggtgac gcgcatgagt cgatcaacac gaatgcatct
     1561 cttctgacct cgaaccaacc cggtcgcgtt gctacgcttg aaggatggac aagagctcgt
     1621 ggcggagcga ggtggcgcgt ctgttggatc actgcgcggc ggcgctacgc gcagggcacg
     1681 atcgtcaagc cctgatttcg ttcggcaccg gtatcgccta cctggtgaag gcaagcgcag
     1741 ctggctcact cgtcgcgggc gagctactcg cgcagctgaa cgaagtcgcg ggcgacggcg
     1801 cgacccagcg tttcacgttc gcgccggggg agctcgagga gctccgcagt cgtcccatca
     1861 acgaatgacc ctcccgggtt cgagggtgag aggttaccgg cgcgaaacgc gtgcgaccgc
     1921 ggaagaatac tttgtcgaga atggattgac cgcaggctga gtctcgtcgc aggcttgggc
     1981 tgacaacgga gctgcctgcc ccggcgtgag gaacgtgact aaggaaagag cggccgaaac
     2041 ggttatcgaa ggtgaacgga tggcgacctc tagcgcggtg ctggttgata cgggcgagtt
     2101 cgaagcggtg accgaagccg agctggttcc ggatgcgccc gaggcagcgg ccagcgcgtc
     2161 ggagacgcat cgcgcgattg ggaaagtcgc ttccgaaccg gcgcgcccga gcggtcaatc
     2221 tggcattcgt ccatctcccg cggccctcgc ggtgcgcatc gaagccgcgc tgcgagcaag
     2281 cacgaagacc gaagcgacgc tcgcggatct acttcgcacg gccaaattcc tgagcgccag
     2341 catcgatgcg gtacgagaag cgaacacggc ggtgttgcgc gagctcgagg tgctctgcga
     2401 gctcgtcgac ggcgagggcg cggagcggag ctctctcgaa cggcgcattc agcgcctgga
     2461 gcatttggtc gacgagacag ggcgcgacgc ggcacgagat cgtcgctttc tcgtcgagga
     2521 acacgatgcc ttcatcgcga ccctcgtgag cgatcacgag cgcgagctcc aggccctgcg
     2581 agagcgccta gccgagctcg agcagcagcc ggctccaagc tccgaacacg aagaagccgg
     2641 cgatcgctcc tgaaggcggc gccgctcacg gcaatagcca gcgcccgtta taaagagctc
     2701 gaatacgggg agctctaggc tagagtgtcg ccgccacgtc ggccgtttgg cccgtggcgg
     2761 aggatgtgta ctcgatgaga gcggctgccg gacccgtgcg caaatggatg ctgaccctcg
     2821 tgatgctgtg ggtcacggcg ttgatgacga cgttcgcgtt gccatcacgg gcagagggca
     2881 acctgctttc agggaagtca ccgtcgcggg ttcaaggcgt ttcgaatgcc aaggtgctga
     2941 ccgacggagc tcgcgcgcac gaaggcgacg agtggaactc gatcgccgcc gccatcatcg
     3001 aatcggatcg cgccttcgtc gtcttcgatc tcggcaaggt cacgcccatc gtcgcggcgt
     3061 acgtgcaagg ggacaacaac gacgagtaca ccctgtccgt ttcggacgac gacaaaactt
     3121 tcactcccct ttggagcgcg gggccgcgca gcgagcctgg tctacgcgag cgctacaacg
     3181 actcgttgac tggccagggc cgctacgtgc gcttgaccgc tcgcggcggt gaccgcgcct
     3241 actcggtgac ggagctgcag atcttctccg agaaaccgaa gacgccgccg gcgctcgggc
     3301 gcgtgtccgg agagagccag gcgtcgcacg tgcgcacctc cctgctttac gtcgcggccg
     3361 ctttcgcgct ctggcttttc acgacgtggg cctccgccag caccgtcgcg atgctgattg
     3421 gcgctgccgc cgtgctcgtc gctggcggca tcgcttacga ggcggtcgac tcggcgtggc
     3481 cgctcgccaa ccgcgaggtc gcttacgtac gcgcggtctc ggcggcgatc gcgttgctcg
     3541 cggtcctgcg ccgcgtcgcg gccgccaagc gtttccccgc gcaccgtcac gtcgtgacgt
     3601 tggctctctt ggcgagcgcg gtgatggcgt tcgcttcgtt ctacaacctg ggtcgcccgc
     3661 agttctggaa tcacaaagag aaccggcccg agttcgtgca cacctacgac atgcgggtgt
     3721 accagccgtt cgcgcgcttc ttcaaagagc tccagtacga cggcgtctac ctcgcgagcg
     3781 tgctggcctt cgccgaagat caacgccacg gctcgctcga gtcgatggcg aacgtggaag
     3841 ttcgcagcct gcgcgaccac aaggtgcaac gcgtcggcga catcgggcct caaatccagg
     3901 agctccgcaa gcgcttcagc gatgaccgat gggccgagct gaagaaggac atgctctact
     3961 tcgaggacgt gatgggccct gagtttctgg gcacgctcac ggaccacggc gcgaacgcaa
     4021 cgccggtgtg ggtgttcttc gcgcggctga tgctcggtca cgctcctgcg agtgagaagc
     4081 tcctgacgtg ggcggggctc gcagacggag cgctcttgct cggcatggcg ttcgccatgt
     4141 ggcgaagctt cggcctctgg ccggcattgc tcgcgatgac cgtcttcggg gcgaacgatc
     4201 tctacatgtt cgggacgaac tggagcggcg cgacgttgcg ccacgactgg ctcgcgctgc
     4261 tcggctttgg cgcctgcgca ttgaagagcg aacgctgggt cgtcgccggc gtgctcctcg
     4321 cgctcgcagc gatgatccgc gccttccccg cggcggcgct gctgggcgtt tcgatcccgg
     4381 cgatttggtg gctctacgag tattggcaag cagagcgcaa gatccccaat taccgggatg
     4441 tgtacgaagc gcacccggag accgtgcgcg tgctgctcgc cgcgatcggc acgatggtgt
     4501 tcgcgttcct gttcactggt ttgctctacg gcttccatgc gtgggggaac tggtgggaga
     4561 aggtgacgct cttgaaccgc gacgtgggcg tcaacgagat cagcctgcgc gcgctggtgg
     4621 cgggcgccga cgggaacgcc gggcgcgtgc ttcaggcgcg ccgcgcgatc cacatcgcgg
     4681 cagttctgct gagcgtcggc acggtgttcg cgctcggtcg cggccgcccc ctgcaccagg
     4741 ccatgctcat cgggatgccg ctcgcgctcg tgatttggaa cccctcgaac tactactcgc
     4801 acttcgtctt cttgctcgtg ctgctcggca gcgtgacggt gccccgcaag tcgaaggtca
     4861 ccgaaaagag cgaagacgac gagcaagagg aagagccgaa gttccagccg atctgggcgc
     4921 agcttcacct cacggccgcg cccctcctcg tcatgtgcat cgcggggtac tggtcggcgc
     4981 tagagcccga cctcgagcgt cactttcaaa acgaaaccgt gctgttgttc gcggcctttg
     5041 cgtgggttta cgcgaacctg ctcaagcgcg accccaagtt gaagagcgcg ctcatgaact
     5101 gagcgtgggg cgacgtttgc ggccgtagct cgcaccgagc gccgcgaaca gcgccccgcc
     5161 gccgatgaag atgcaagcgg cgccgacggc catcgccgag gaacccaccc ctttcaacag
     5221 cgagaagcca acgagcagcg cgccgccgag cgcccccgcg cccgcgggaa ccgcccggtg
     5281 cgcgcgctga ccaaagcgcc ccacgactgc gcccgctccc gctgccgcaa ccacgtaggc
     5341 caagagcacc ggcagcgccg cccagccgag cgcggccgtt tccgagcgcg cgagccctcg
     5401 cgcgagccgg gagcccgccc agagtccgac gagcgacaac ggcaagaagg tcacgaccgt
     5461 gaaggcggcc ccgatgagga gccactgcgg gctgctccgc gaaagcgcgg ccgcctcatc
     5521 ggagggcgga ctctggatga cgggaaggcg tcgagccacg gctcgaacgt ggcggacgca
     5581 aaagaaaggc gcaactgtgg cacgcgcggg gccgacgttg cggcatgtcc ctttcgctcg
     5641 ttttggcgct ccccttggcg ctcgcacccg ccacgctcgg ccacattggc gaacgtccac
     5701 cggcgctcgc ggagcttccc gccccggcgg ccccggtcga tgaaccgcca gagctcccac
     5761 gccgaacgtt cgagctctgg cccgcggccg gcctcagttt gcctcaatgc ggtgtgagca
     5821 gcggcaccgg ctgcgcggcg ctgagcgcag gcccagagct tgggctcgtc ggcttgatgc
     5881 gaccactgcc gcacttcgcg ttcggcgcga gcgcacgccg ggccaccttt tcagcgaagc
     5941 cggcagaaga cacctggcgc acctcggcga cgatgtcgct cttttcggtg gttgggcggg
     6001 tgtacgcttt cgaaggcggg cgcttcgatc cttacctcga gctcgatctc ggcgcgggct
     6061 cgctcggtgt cgaagtgacg gggcacggcg cacataagga agaagcgctg ttttcaccgg
     6121 cggttcgctc ggtgctcggc gtcgacgtcg tactgagtgg gtggctacgc gcgggcgcgt
     6181 tcctcgccta cgctcgctac tttccgggca cggtgtcgtt ctgcagcgat ctcgggtgcg
     6241 gcgcggtggc ggcttcgaac agcgggctta cgctcggctc gctgtcgctc ggggtcgaag
     6301 tggggctcgt tggcggagaa accttgtagc cgcgcgcttc gctccggtgt tacgttgaac
     6361 ccatgggcgg acgattcggg gttcgagtgt gcgggctttt gctagcgctc gcgctgtgcg
     6421 ccgcgt
//
LOCUS       DASTKS010000789         5135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_794426, whole genome shotgun sequence.
ACCESSION   DASTKS010000789 DASTKS010000000
VERSION     DASTKS010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794426"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFQ35_16315"
     CDS             complement(<1..520)
                     /locus_tag="VFQ35_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932270.1"
                     /translation="MKRISLRNHACRLFNLSAHGALLSLPLLLACSSSGSGGSGDGGS
                     TSASGGATSSGSGGASTGQGGNTASGGSTSASGGSTNANGGNTAKGGSSSESGGNTAK
                     GGSTGSGGSTNQSGGSTSTTGGNTAKGGGTGTAGAGGGDKCPTDAEEKFSFFLTSNAG
                     LIRESGKADGFGG"
     gene            complement(810..3146)
                     /locus_tag="VFQ35_16320"
     CDS             complement(810..3146)
                     /locus_tag="VFQ35_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9932271.1"
                     /translation="MTALELARRRRLLERGLPWLVCGLALAGAAIAASALIDLGILSE
                     YQLFPSIVRGYTPSGAAFGVLAVLFTLGTLFYSLRRRSLQETLPIGRGTLAMWLWSHV
                     TLGGLALVFACVHAGYGAFSLQPSFGKALLALLFVVSASGAFWRLVYGFVPRRAARTL
                     GNYAADESLSRARAQAVEIDKLAAGRSPRLRALVDRVLAVPIDAAEAGRSTAALDPSE
                     AAVFADIARLASERHAALARARGQERALSRLQGPRVWHVPVSLLFALLVPLHVFSAYD
                     VPERLFAKTPSTAALAFEPSTTCSRCHAQIVKEWQSSMHAHALDGPLMRAQTNVAART
                     SLASASGPDPKLICVNCHGPVARLLSPSPTLPLAAPALAAPEQVHEGITCVACHAQRA
                     EPRTGGAGLSAFAASLAPGRTYFGPRDDAVGNAFHRSERSAHFEAPERLCQNCHSVVY
                     DLDGDGRIEKGKDLVLQDLFSEWQAYRAAGGASCIDCHMPPTAPGRSAERADVPFEQD
                     GDAPPRARRSHRFVGPDYPLDRPAVRDETRAERHALLASAAKLALSELGSTPEALRFV
                     LTLSNTGAGHGLPGGFAFVRQMWLEVSVFDAQNRLLASSGRLRSVTDDLCDAELLQPG
                     RALARFASGCSEPDRLLVNLQQRLVERIEVDKDASGNARRDARGQAVLAAAANADEVV
                     VQHVTSGAVARLRPFDGQRMPPLDPGSARQFAYTFVLPAGVRAARIEARLLFRAVPPY
                     FLRALANQQSSGDGARLDTWLGNLEIVEMTRVSRNATD"
     gene            complement(3143..4984)
                     /locus_tag="VFQ35_16325"
     CDS             complement(3143..4984)
                     /locus_tag="VFQ35_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9932272.1"
                     /translation="MTIGAASAQSISAYEVDLARRVEAEAMACIGCNDCLLACPIPES
                     QNVTIGELNRALHLPVITAENVARFVTACTQCGQCVPACPADLSRAQMVLYNKLKVED
                     SVQDFELSLSARTVTFAAGITRNALASELATLELFRDASAQALRRLVQKSTLRLLVPG
                     EVLCVEGEFHERLCIVLSGALDQVSRDARGATVQLATLGPGSFFGEMGILGDAPEPFE
                     VVARDKSIVLQSPKVSVLRLLAEAPSVEGTLSELYSRRALFTYAKSPGALGALPDHAI
                     SELFRDARLALLSSGQTLLEKGSSPKEFYLVRSGFLRALQSDAGGERVLTYFREGNVF
                     GILPLLNREPQHRYSVFAVSRAEVICVRANTLAGVLARYPGAGEMLLQGSFEAERLAR
                     ASDVGLRPLRSIAPASSIAPASRSEASHPSLPGERAPLEAAVLVERGIAKGREVLVID
                     QTRCTGCGNCIDACERRHGMSRLQLRGIQAENYLFPSACRHCQDPACLLCSVNGIARL
                     PSGEIQIVTENCIGCGSCAERCPYGSISMHVAQPKRRGFLPTLRDFLAGPTRRLALEA
                     IDPSQARVAVKCDLCAHHDDYACVTACPTASAFRMNPETALRGDPAK"
BASE COUNT          865 a         1668 c         1758 g          844 t
ORIGIN      
        1 ctccgccaaa gccgtccgcc ttgccggatt cgcggatcaa ccccgcgttg ctcgtcagga
       61 agaagctgaa tttctcttcg gcgtcggttg ggcacttgtc accgccgccc gcgcctgcgg
      121 taccggtgcc tccgcccttt gcggtgttgc caccggtcgt gctggtgctg ccgccgctct
      181 ggttcgtgga acctccggaa ccggtgctcc cgcccttcgc ggtattgccg ccactctcgc
      241 tgctgctgcc acccttggcg gtgttcccac cgttcgcgtt ggtgctaccg ccactcgcgc
      301 tcgtgcttcc gcccgaagcc gtgttcccgc cctgcccagt gcttgctcct ccggagccgg
      361 agctcgtcgc gcctccgctc gcgctggtag agccaccgtc gcctgaccct ccgctgccgc
      421 tgctggaaca cgcgaggaga aggggcaacg acaacaacgc cccatgcgcc gagagattga
      481 acagacgaca agcatgattg cgaagagaga ttcttttcac tgattgctcg cggtgttgtg
      541 gctgggatgt cgctcgtcaa ggacggtcag aactttgaag tcactcggaa gcgacggctt
      601 gggtcgtctt gatacagtgt tcgatttgtc tattgagtgt cacgtggagt taacaagaat
      661 ttcaacattc cgtgcgctga gaatattcgc gtgctcgtgg aagtatcccc agcattacgc
      721 cctgattttt tgccgtctct aaccgtgctt cggctctcgt ggcgcgggca gcagctgcga
      781 gcggacacga gtgtcgtcga tgcgatcgct cagtcggtcg cgttgcgact gacgcgtgtc
      841 atctcgacta tttcgaggtt gccaagccag gtatcgagtc gtgctccgtc accgctcgat
      901 tgctggttcg cgagagcgcg caggaaataa ggaggcacgg cgcgaaataa tagcctcgcc
      961 tcgatccgcg ccgcgcgaac tccagcaggt aggacgaagg tgtacgcgaa ttggcgtgcg
     1021 ctgcccggat cgaggggcgg catgcgctgc ccatcgaaag ggcgaaggcg cgcgacggca
     1081 ccggaagtga cgtgttgcac tacgacttcg tcggcgttcg cggcggcggc caacaccgct
     1141 tgtccccgcg catcacggcg cgcgttcccc gaagcgtctt tgtcgacttc gatgcgctcg
     1201 acgaggcgct gttggaggtt cacgagcaag cggtcgggtt cgctgcagcc gctcgcgaac
     1261 cgcgcgagcg cgcggcccgg ttggaggagc tctgcgtcgc aaaggtcgtc agtcaccgaa
     1321 cgcaatctac cggagctcgc gagcaaccgg ttctgagcat cgaacacgct cacttcgagc
     1381 cacatttgcc gcacgaacgc gaacccaccg ggcaagccgt gcccagcgcc cgtgttcgag
     1441 agagtgagga cgaagcgcag cgcctctggt gtcgaaccga gctccgacaa ggcgagcttc
     1501 gccgcgctcg cgagtagcgc gtgccgctcg gcgcgcgtct cgtcgcgtac cgcaggccga
     1561 tcgagcgggt agtcagggcc tacgaagcga tggctgcgcc gcgcgcgggg cggggcatcg
     1621 ccatcttgct cgaagggaac gtcggcgcgt tcggcgctgc gtcccggggc cgttggcggc
     1681 atgtggcagt cgatgcagct cgcgccgccg gcggctcgat aggcttgcca ctccgagaag
     1741 aggtcctgca aaacgagatc ttttcccttc tcgatgcgtc cgtcgccgtc gaggtcgtag
     1801 acgaccgaat gacagttttg gcaaaggcgc tccggggcct cgaagtgcgc cgagcgctcg
     1861 ctccggtgga aagcattgcc gaccgcgtca tcgcgcgggc cgaaataggt ccggccaggg
     1921 gcgagcgaag ccgcgaaggc gctgagcccg gcgccgcccg tacgcggctc ggcccgttgg
     1981 gcgtggcacg cgacacacgt aatgccttcg tgcacttgtt ccggcgccgc cagagccggg
     2041 gccgcgagcg gcagggtcgg acttggcgag agtaggcgag ccaccgggcc atggcagttc
     2101 acgcaaatca gtttggggtc gggccccgac gccgacgcga ggctggtgcg agccgcgacg
     2161 ttggtttgag cgcgcatcag cggcccgtcg agcgcgtgcg cgtgcatcga gctctgccac
     2221 tccttcacga tttgcgcgtg gcaacgtgag caggtcgtcg acggctcgaa cgcgagcgcg
     2281 gccgtgctcg gcgttttcgc gaacagtcgc tcgggaacgt cgtacgcgga gaagacgtgc
     2341 agaggaacga ggagcgcgaa cagcaagctc actgggacgt gccagacgcg cggaccctgg
     2401 aggcgagaca gcgctcgctc ctggccgcgc gcacgagcga gcgccgcgtg acgttcactc
     2461 gcgagtcgcg cgatgtcggc gaacactgcc gcctcgctcg gatcgagggc agcggtgctg
     2521 cggccggcct cggccgcgtc gattggcacc gcaagcaccc gatccacgag cgcgcgcaga
     2581 cgaggtgagc gcccggccgc gagtttgtcg atttcgaccg cttgcgctct ggcgcgcgag
     2641 aggctctcgt ccgcggcgta gttgccgagc gtacgcgcgg cgcgccgtgg cacgaaccca
     2701 tagacgagac gccaaaacgc gccactcgcg ctcacgacga agagcagcgc gagcagcgcc
     2761 ttgccgaatg aaggctgtag gctgaaggcg ccgtagccgg cgtggacgca cgcgaaaacc
     2821 agcgcgaggc cgccgagcgt aacgtgcgac cagagccaca tcgcgagcgt gccgcgcccg
     2881 atgggcaggg tttcttgtag ggagcgccgc cgcagcgaat agaacaacgt gccgagcgtg
     2941 aagagcaccg cgagcacgcc gaaggcggcg ccgctcggcg tgtagccccg cacgatcgac
     3001 gggaacagct ggtactcgct caagatgccc agatcgatca gcgcgctcgc agcgatcgcg
     3061 gctccggcga gcgcgagccc gcacacgagc cagggcaggc cgcgctccag caagcgtctg
     3121 cgccgggcga gctcgagcgc cgtcacttgg ctggatctcc gcgcagggcc gtctccgggt
     3181 tcattcggaa agcggatgcg gtcgggcacg ccgtgacgca tgcgtagtcg tcgtgatgcg
     3241 cgcagaggtc acacttcacg gccacgcgtg cctggctcgg atcgatcgcc tcgagcgcga
     3301 gtcgtcgggt agggccggcg agaaagtccc gcagcgtcgg cagaaacccg cgtcgcttcg
     3361 gttgagccac gtgcatgctg atgctgccgt aggggcagcg ctcggcgcac gaaccacagc
     3421 cgatgcagtt ctccgtgacg atttgaattt cgccgctcgg gagccgcgca atgccgttca
     3481 cgctacagag caggcatgcc gggtcttggc agtggcggca cgcgcttgga aaaaggtaat
     3541 tctccgcctg aatgccgcgc agctgaaggc ggctcatgcc gtgcctccgt tcgcaggcgt
     3601 cgatgcagtt gccacaaccg gtgcagcgag tctggtcgat gaccagcact tcgcgcccct
     3661 tggcgattcc gcgctcgacg aggacggcgg cttcgagtgg ggcgcgctcg ccgggtaggc
     3721 tcgggtgcga cgcttcggag cgcgatgccg gcgcgatgct cgaagcgggc gcgatgctgc
     3781 gcagcggacg gagcccgaca tcgctcgcgc gcgcgagccg ttccgcttcg aaggagccct
     3841 gcaagagcat ctcgcccgcg ccgggatagc gcgccaaaac gcctgcgagc gtgttcgcgc
     3901 ggacgcagat tacctcggcg cggctcaccg cgaacaccga ataccggtgt tggggctcgc
     3961 ggttcaggag cgggaggatg ccgaacacgt ttccctctcg gaagtaggtg agaacgcgct
     4021 caccgcccgc gtcgctctgc aacgcccgca agaagccgct ccgaaccaag taaaattcct
     4081 tgggcgagct acccttctcg agcaacgttt gccccgagga gagtagggcc agacgcgcgt
     4141 cgcggaaaag ctcggagatc gcgtggtcgg gcagcgcgcc gagcgcgccc gggctcttcg
     4201 cataggtgaa gagcgcgcgt cgcgagtaga gctcgctcaa cgtcccttcg acgctgggag
     4261 cttccgccaa caagcgcaac accgagacct tcggcgactg gagcacgatg ctcttgtcgc
     4321 gagcgacgac ttcgaagggc tccggcgcgt cccccaagat gcccatttct ccgaaaaagg
     4381 agccggggcc gagcgtcgcg agttgaaccg tcgcgcctcg agcgtcgcgc gatacctggt
     4441 cgagcgcgcc gctcagcacg atgcaaagcc gctcgtgaaa ctcgccttcg acgcagagca
     4501 cttcgccggg gacgaggagc cgcagcgtgc ttttttgcac gaggcggcgc agcgcctggg
     4561 cgctcgcatc ccgaaacagc tcgagcgttg cgagctcgct cgcgagtgcg ttccgcgtga
     4621 taccggctgc gaaggtgacc gtgcgcgcgc tgagcgagag ctcgaagtcc tggacgctgt
     4681 cctcgacctt gagcttgttg tagagcacca tctgcgcgcg gctcaggtcc gcggggcacg
     4741 ccggaacgca ttgaccacac tgcgtgcacg cggtcacgaa tcgagccacg ttctcggcgg
     4801 tgatcacggg caagtgcagc gcccggttga gctcgccgat ggtgacgttt tgcgactccg
     4861 ggatggggca ggcgaggagg cagtcgttgc aaccgatgca ggccatcgct tcggcttcga
     4921 cccggcgcgc gaggtcgacc tcgtacgcgc tgatcgactg ggcagaagcc gcgcctatcg
     4981 tcatgtttcc tcgaaccgga gcaggcgagg gccggagagc atcaagaggt cgccgtgttc
     5041 gagcggcacc cactcggtgc caagctgccg cccggagcgc agcgtgcgtt ggccgatgtc
     5101 ccgcacgaaa aagccgcgac cgtgctggac gattt
//
LOCUS       DASTKS010000790         9642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1912441, whole genome shotgun sequence.
ACCESSION   DASTKS010000790 DASTKS010000000
VERSION     DASTKS010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912441"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VFQ35_16330"
     CDS             complement(<1..1578)
                     /locus_tag="VFQ35_16330"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HET9932273.1"
                     /translation="MGPLWLEFDKNGLGTSAAPSPFHSEPGGATASPGHAAHISGTLG
                     ANKAPWSWVQLQVHFNANQAPEDLSGYKTLRFFVKGNGGRYAVAFAKASVTDHDHFRY
                     EFTAPSAWTEVSVPLAELRQAGWGKRVAARFDDVVRIYFSPVEFDKPFDVVIDQVVLS
                     PSETKLEAKPYATADWFAWSGIDVAKRRGTALDVSRLLDAPAGKHGALARKGEAFVFA
                     DGKRTRFWGVNIVASANFPSHEEADKLAEFLASLGVNMTRHHHIDAAWSTPNVFGNKS
                     STLALDAAAMDRFDYFVAALQKRGIYQYFDMLVHRKVTAEDGVPGADKFIAGLKIEGE
                     FEPKLIELEEKFIDAFMGHENPYTKQTYAKNPAIALVDIINEDSLFWIRNEGDFAVKS
                     EASKRELGRQFSAWLAQRVPGGRAALEARWADAGGKQGLSQKEDPAQGSVEFAFAFEP
                     ETYQRFSRARLADTLRFLYDTQLSYYRRLQARLKRVGYRGLVTGSNHWTEHPLDLAAN
                     SELDFVDRHSYFSHPEGG"
     gene            1886..3082
                     /locus_tag="VFQ35_16335"
     CDS             1886..3082
                     /locus_tag="VFQ35_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9932274.1"
                     /translation="MIPVSVGLGDKSLFASLEADAYLGYAAVAPVSRLVQSTLEELMR
                     DCAHTGQPALLRWYERRERLREKLSALIDASPEQVAFTGGTTQGLNALALCFPWERED
                     TVLLVDGEFPANVTPFQNAARLFGLRVRFLPKPDPREPEAFLRALEAALRERVRLVAL
                     SAVCFQTGLAMPLERIAELCREYDAELSVDAIQALGVVPLSVRKLKVDYLVGGAHKWL
                     MGIEGAGFLFARAERVRKLIPRNAGWLSHEDGLRFLFTGPGELRYDRPIKSGLQFMEG
                     SSMSTFGFAALEAGLDPIQELSVPRIFAHVNNYLDALEPGLVERGFASFRAQDPTRRS
                     GILSTLPPRGIKAGELVQALRMRRVIATMPDGLLRFAPHFPNSLDEVPLVLAALDGAL
                     DEVKSR"
     gene            3201..3818
                     /locus_tag="VFQ35_16340"
     CDS             3201..3818
                     /locus_tag="VFQ35_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9932275.1"
                     /translation="MKLYVNPLSPNCRRILAVADYLGLTPEIKVVDFSKGEHRSAEFL
                     AVNPNGAVPVLEDGDWILTESRAILHYLSSQRPEARLVPTDAKELAEVMRWQFWDAAH
                     FGPPNGTAFFQRVLKPMTGGDPDESRVRDAEAQFQRYAKVLDEHLANRAWLVGRSITV
                     ADFTVAASLAQGMAAGISLEAFPNVRAWYAHLAELDAWRNTEPKS"
     gene            complement(3832..4455)
                     /locus_tag="VFQ35_16345"
     CDS             complement(3832..4455)
                     /locus_tag="VFQ35_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932276.1"
                     /translation="MNSVIESATTGRSKCRGCGRAIAKGDLRYGDSVPNPYAEGETLV
                     WFHLGCAACMRPEPFLPVLKAHSPSPPDADWLLQVAEKGVKHPRVTRLGHAERAASGR
                     AHCRSCRETIDKGHFRIALQSFEDGRMGPIGSIHIECAEAYFGTSDILDRIERLTPEL
                     DAEAITEIRAGLANQRPAPPPEEEEPRLAKTSGEGGDAEAGTEKKQA"
     gene            complement(4506..4769)
                     /locus_tag="VFQ35_16350"
     CDS             complement(4506..4769)
                     /locus_tag="VFQ35_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HET9932277.1"
                     /translation="MALADDFAEAQTRVKTLKTTPSPDELLELYALYKQATVGDVQGE
                     RPGMLDFKGRAKYDAWAARKSTSSEQAMTGYVELVNRLAKKYG"
     gene            complement(4981..6669)
                     /locus_tag="VFQ35_16355"
     CDS             complement(4981..6669)
                     /locus_tag="VFQ35_16355"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9932278.1"
                     /translation="MSFEPQRYVAPERVHLPDRRWPERALTAAPVWCSVDLRDGNQAL
                     PDPMGHARKQALLELLVKVGFKQIELGFPSASRTDFDFIRWVIEHGVLPSGVIPQVIT
                     QSREALIARTMESVVGAERAIIHLYNSTSELQRRVVFGMSRQQIVDLALSGTRQIRER
                     AAALKGTRVTFQYSPESFTGTELDFAAEICNAVIEAWEPTPSEPMIINLPSTVENTTA
                     NVFADRMEWMSRNLARRDSVVLSVHAHNDRGSGVASAELAMLAGAERVEGTLLGNGER
                     SGNVDIVTLAMNLYSHGVDPGLRFEDMREVVRIVEESTRLPVHPRHPYAGELVFAAFS
                     GSHQDAIHKGFKHMASAKPTQWEVPYLPIDPADIGREYEPVIRINSQSGKSGLLHVLE
                     RDYGYRVPRELAIEFSKRVQGVTDERGEELSSAAVHELFEATYLRAPGAVAVSSVAIE
                     RRANGRECAVSAEISADGCARTVQGIGSGPIEAFARAVALVVGEPFDVVDYAEHARGA
                     GADAEAVAYVALQIRGAVYYGVGSNADVVLASLHALVSALNRAGCSARSAAESG"
     gene            7041..8645
                     /locus_tag="VFQ35_16360"
     CDS             7041..8645
                     /locus_tag="VFQ35_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932279.1"
                     /translation="MRASLRSVSFALSFCFSACGGDNHPGMDTAAGASSLGGSAGQNS
                     ARGGSAGSVSIETGGSSGATSGGAGGSAQGTAASAFRYGFNAGYPNPKFNDQDLAALG
                     VRAGATSERVSLPEQHLERWGYDIEVADVKAYRTLGMGSLVGFLTSPTRAHSTAPADA
                     SDDQVGQYIPKNLYEPITTANGEVNPNNYWASYVYRTVSTYGSWIDTWEIWNEPDWVS
                     DWKVTEAWATRAPSAADLPRFHGSIFDYVRMLRVSSVAAKLANPNAKIATGGIGYPNF
                     LDAIVRYSDEPKAGAIDADHPATGKAYFDVVSFHHYPIYTPGNSEAGVDGFMKQRQAL
                     GALVDPSRVLGWECTETGAPHLPLDGIPSGSAYAKNYLLKVMALAPSADLGGVHWFVL
                     SDAKAAGQSTDPYAFMGLYAPVASLSTPSEAELSESGVAYATLSQLLRGAHYDGIATS
                     ALGLPGTARGIAYRSADDARILVLWAIAPADSETASTSVELASPRTLRQHAWDFSKTG
                     QSQLLTPVNGVVTLALEATPRIFVEQ"
     gene            complement(8866..9381)
                     /locus_tag="VFQ35_16365"
     CDS             complement(8866..9381)
                     /locus_tag="VFQ35_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932280.1"
                     /translation="MALKHLAAFAFAIVLGSACSAGTEPGEGSAVERDAVTKVCVQNV
                     LCIQGSHWDPVACQCVPDQCVSGPGGPCGGFTQNPCVCRAGTKCVLNHIPDLPGVCAV
                     CDPIKCPSGQRWDAAACQCVSGCRTAADCHGLLPQFCKQCADGTYGCAHHACVAGKCE
                     IAFCADGGTTM"
BASE COUNT         1695 a         3273 c         3004 g         1670 t
ORIGIN      
        1 cccaccttcg ggatggctga agtaggagtg gcgatcgacg aaatcgagct cgctgttcgc
       61 ggcgaggtcg agcggatgct cggtccagtg attgctaccc gtgacgagcc ctcgatagcc
      121 gacgcgcttc agtcgcgctt gcaagcgacg atagtacgag agctgcgtgt cgtagagaaa
      181 gcggagcgtg tccgcgaggc gagcccggct gaaccgctgg taagtctctg gctcgaacgc
      241 aaacgcgaac tcgacgctgc cctgcgccgg atcttccttc tgcgagaggc cctgcttgcc
      301 gccagcgtct gcccaacgcg cctcgagcgc ggcccgtccg cctggcacgc gctgagcgag
      361 ccacgccgag aactgccgcc cgagctcacg cttgctcgcc tctgacttca ccgcgaagtc
      421 gccctcgttt cgaatccaga agaggctgtc ttcgttgatg atgtcgacga gcgcaatcgc
      481 cggattcttc gcgtaggtct gcttcgtgta cgggttctcg tgccccatga aggcgtcgat
      541 gaacttctct tcgagctcga tcagcttggg ctcgaactcg ccttcgattt tgagtccggc
      601 gatgaatttg tccgcgccgg gcacgccgtc ttcggcggtc accttgcgat gcacgagcat
      661 gtcgaaatac tggtagatcc ctcgcttttg cagcgcggcc acgaagtagt cgaagcggtc
      721 catcgcggcg gcatcgagcg cgagcgtgct cgacttgttg ccgaacacgt tcggcgtact
      781 ccaggccgca tcgatgtgat ggtggcgcgt catgttgacc ccgagcgacg ccaagaactc
      841 ggcgagcttg tcggcttctt cgtggctcgg gaagttcgcg ctagcgacga tgttgacgcc
      901 ccagaagcgc gtcctcttgc cgtcggcgaa cacgaacgct tcaccttttc gagccagcgc
      961 gccgtgcttg ccagcgggag catcgagcag tcggctgaca tcgagcgccg ttccgcggcg
     1021 cttggcaaca tcgatgccgc tccaagcaaa ccaatccgcc gtcgcgtacg gcttcgcctc
     1081 gagcttggtc tcgctcgggg agagaacgac ctggtcgatg acgacgtcga acggcttgtc
     1141 gaactcgacc ggcgagaagt agatccgtac gacgtcgtcg aagcgcgcgg cgacccgctt
     1201 accccaaccg gcttgccgga gctcggcgag cgggaccgag acttcggtcc acgccgacgg
     1261 cgccgtgaat tcgtaccgga agtgatcgtg atccgtgacg gacgccttcg cgaaggccac
     1321 ggcgtagcgg cccccgttac ccttcacgaa aaagcgcagg gtcttgtagc cggagaggtc
     1381 ctcgggcgct tggttcgcat tgaaatgcac ctgaagctgc acccacgacc agggcgcctt
     1441 gttggccccc aacgttccgc tgatgtgtgc cgcgtgtccc gggctggcgg tcgccccacc
     1501 cggctcggag tgaaatggag agggcgccgc actggtccca agtccgttct tgtcgaattc
     1561 gagccagagc ggtcccaagt gaccctgtcc ggtctcgaaa tcctccagta accaatctcc
     1621 cttggggatc ttggcagcag aatttgcgct ggaggcagcg ggggcggcgg gattcggcgg
     1681 tggcgcgcat cgcaccgggg cgcacgcgac gagccacgga gcgccgagag ctgcaagaag
     1741 aacgagtggg cgaagggagc gattcgattc caggcgcatg cggttcgggt ttaacactcg
     1801 ttcgcctcgg gcacctcgcg aataaagttc gactttccat cagaaagtct cgacgcgccg
     1861 agtctgcgct gccaaaaggg catacgtgat cccggtatcg gtcggactag gggacaagag
     1921 cttgttcgcc tccctcgagg cggacgccta cctcggctac gcagcggtcg caccggtgag
     1981 ccgcctggtc caaagcacgc tcgaagagtt gatgcgcgac tgcgcgcaca ccgggcaacc
     2041 tgcgctcttg cgctggtacg aacgccgcga acgcttgcgc gaaaagctga gcgccctcat
     2101 cgacgcatcg ccggagcaag tcgcattcac gggtggcacg acccaggggc tcaatgcgct
     2161 cgcgctttgc ttcccctggg agcgcgagga cacggtgctg ctggtcgacg gtgagttccc
     2221 cgcgaacgtc acgccgtttc agaacgctgc acgcctattc ggcctgcgcg tgcgtttttt
     2281 gccgaaaccg gatccgcgag agcccgaagc cttcctgcgt gcgctcgagg ccgcgctgcg
     2341 cgaacgcgtg cggctcgtcg ccttgagcgc cgtttgcttt caaaccgggc tcgccatgcc
     2401 gctcgagcgt atcgcagagc tctgtcgcga atacgacgcc gagctctcgg tcgacgcgat
     2461 ccaggcgctc ggggtcgtgc cgctcagcgt gcgaaagctc aaggtcgact acttggtcgg
     2521 cggcgcgcac aagtggctga tgggcatcga gggcgcgggc tttctcttcg ctcgcgccga
     2581 gcgagtgcgg aagctcattc cgcgcaacgc cggatggctg agtcacgaag acgggctgcg
     2641 ctttctgttc accggtccgg gcgagctccg ctacgaccgt cccatcaaga gcggcctcca
     2701 gttcatggaa ggcagctcga tgagtacatt cggtttcgct gcgctcgaag cagggctcga
     2761 ccctattcag gagctctccg ttcctcgcat ctttgcgcac gtgaacaact acctcgacgc
     2821 gctcgagccg gggctcgtcg agcggggctt cgcgagcttt cgcgctcaag acccgacacg
     2881 tcgctcgggc attctgtcga cgctgcctcc gcgcggcatc aaggcggggg agctggttca
     2941 ggccttacga atgcgtaggg tgatcgccac catgcccgac ggactgctgc gttttgcgcc
     3001 ccattttccg aactccctcg acgaagtgcc cctggtcttg gccgccctcg acggcgcgct
     3061 cgacgaagtg aagagccgct agtcgccatt cgcaaccgca aacccgcccc gcctctgacg
     3121 gttgctgcgc tctctgccgg tgccgccttg ctccacggca cgaggacgag ctacacctgg
     3181 agccgcggtt ggaactcccc atgaaactct acgtgaatcc gctctcgccg aactgtcgtc
     3241 gcatcctcgc cgtcgccgat tacttggggc tcacccccga aatcaaagtg gtggacttca
     3301 gcaaaggcga gcatcggagc gccgagtttc tcgcggtgaa cccgaacggg gcggtcccgg
     3361 tgctcgaaga cggagattgg atcctcaccg agtcccgcgc catcttgcac tatctgtcct
     3421 cgcagcggcc cgaggctcga ctcgtcccga ccgacgcgaa ggagctcgcc gaggtgatgc
     3481 gctggcagtt ctgggatgcc gcgcacttcg gtccgccgaa cggcaccgcc ttctttcagc
     3541 gcgtgctcaa gccgatgacc ggcggggatc cggacgagag ccgcgtacgc gacgcggaag
     3601 cgcaatttca gcgttacgcc aaggtgttgg acgaacacct cgcgaaccgc gcctggctcg
     3661 ttgggcgtag catcaccgtc gcggacttca ccgtcgccgc ctcgctcgcg caaggtatgg
     3721 ccgcaggcat cagcctcgaa gcgttcccca atgttcgcgc ctggtacgcg cacctcgccg
     3781 agctcgacgc ttggcgcaac accgagccga agagctgagc tttcgcggcg ctcacgcctg
     3841 ttttttctcg gttcctgctt ccgcgtcgcc cccctcaccg ctcgtcttcg caaggcgcgg
     3901 ttcttcttcc tcgggcggcg gcgcgggacg ttggttcgct agccccgcac gaatctccgt
     3961 gatcgcttcg gcgtcgagct ccggggtgag ccgctcgatg cggtcgagga tgtcgctcgt
     4021 gccgaaatag gcctcggcgc actcgatgtg aatcgagccg atggggccca tgcggccatc
     4081 ctcgaaagac tgcagggcga tgcggaaatg tcccttgtcg atcgtttcgc ggcacgagcg
     4141 acaatgcgct cgccccgaag cggcgcgctc cgcgtgcccg agccgcgtga cccggggatg
     4201 ctttaccccc ttctcggcca cctgcagcag ccagtcggca tccggcggag agggagaatg
     4261 agccttgagc acaggaagaa acggctcggg gcgcatgcac gcagcgcacc cgagatgaaa
     4321 ccaaaccagc gtctcgcctt ccgcgtaggg gttgggaacg ctgtcgccgt agcgcagatc
     4381 tcccttggct atggctcgcc cgcacccgcg gcatttggaa cgtcccgtag tcgccgattc
     4441 gatgaccgag ttcacgaccg aattttccgg cgaacggacc cgcgggtcaa acttttgaag
     4501 cctgctcagc cgtatttctt ggcgagccgg ttcacgagct cgacgtagcc agtcatcgcc
     4561 tgctcgctcg aggtggattt gcgggcggcc caggcgtcgt acttggcccg acctttgaag
     4621 tccaacatcc ctggcctctc gccctgcacg tcgccgaccg tggcctgttt gtagagagcg
     4681 tagagctcga ggagctcgtc ggggctcggc gtcgtcttca acgtcttcac gcgcgtctgc
     4741 gcctctgcaa aatcgtcggc gagggccatt ggtgcagtct agagaagcgg ctcgtcgaag
     4801 gcgagttttc gaattcgacc ataacgcacg gccaaccgcg ccccgaaggc gacacggagc
     4861 cgacgagcag acgaaccgct cgccgccgac gtggggcgtc gatgtctcga cggctaggag
     4921 ccgccgcagg gcgtctagtc gacattgcga acgagacgcc ccgcgcatcg agcggttccg
     4981 tcaaccggat tcggccgcgc tgcgcgcgct gcacccggcg cgattcagcg cggacacgag
     5041 cgcgtggagc gacgccaaaa cgacgtccgc attcgagccg acgccgtagt acaccgcgcc
     5101 gcggatctgg agcgcgacgt aggcgaccgc ctcggcgtcc gcacccgcgc cacgtgcgtg
     5161 ctcggcatag tcgacgacat cgaacggctc gcctacgacc aacgcgacgg cgcgcgcaaa
     5221 cgcttcgatc ggccccgacc caatgccctg caccgttcga gcacatccat ccgctgaaat
     5281 ctccgcgctc accgcgcatt cacgcccgtt cgcgcgccgt tcgatcgcga cgctcgacac
     5341 cgccaccgcc cccggcgcac gcaggtacgt cgcctcgaac aactcatgga ccgccgccga
     5401 agagagctcc tcgccgcgtt cgtcggtaac accttgaacg cgcttactga actcgatggc
     5461 gagctctcgc gggacccgat agccgtagtc gcgctccaaa acgtgtaaga ggccgctctt
     5521 gccggactgg ctgttgatgc ggatgaccgg ctcgtattcg cgcccgatgt cggcgggatc
     5581 gatgggcagg tacggcacct cccactgcgt gggtttcgcc gacgccatgt gcttgaaccc
     5641 cttgtggatc gcgtcctgat gcgagccgct gaacgccgcg aacacgagct cgccagcgta
     5701 cggatgacgc ggatgcaccg gtaagcgcgt gctctcttcc acgatccgca cgacctcgcg
     5761 catgtcctcg aagcggagcc ccgggtcgac gccatgagag tacaggttca tcgccagagt
     5821 cacgatgtcc acgttgccgc tccgctcgcc gttgccgagc aacgtccctt cgacccgctc
     5881 ggcgccggcc aacatcgcga gctccgcgga ggcgacgccg gagccgcgat cgttgtgggc
     5941 gtggacgctg agcacgacgc tgtcgcgccg cgccaagttg cgcgacatcc actccatgcg
     6001 atcggcgaag acgttcgcgg tcgtgttttc gaccgtcgac ggcagattga tgatcatcgg
     6061 ctcgctcggt gtcggctccc acgcctcgat caccgcgttg caaatctccg ccgcgaagtc
     6121 gagctcggtc ccggtgaagc tctcgggcga atattggaag gtcacgcgcg tacccttcaa
     6181 cgccgccgcg cgttcgcgga tctgccgagt gccggacagc gccagatcga cgatctgttg
     6241 gcggctcatg ccgaaaacga cgcgccgttg cagctccgaa gtcgagttgt acaaatggat
     6301 gatcgcacgt tcggccccga cgaccgactc catggtgcgc gcgatgagcg cctcgcgcga
     6361 ctgcgtgatc acctgcggaa tcacaccgct cggcagcacg ccatgttcga tcacccaacg
     6421 aatgaagtcg aagtcggtgc gcgacgccga aggaaagccg agctcaatct gcttgaaccc
     6481 caccttcacc agcaactcga ggagcgcctg cttgcgcgca tgccccatcg ggtcgggcag
     6541 cgcctgattg ccatcgcgca aatcgacgct gcaccaaacc ggtgcggcag tgagcgcgcg
     6601 ctccggccat cgacggtcgg gcaagtgcac gcgttcgggg gctacgtaac gttggggctc
     6661 gaaggacatc gtttttctcg tttgggacct gttaagggcc ggagaaaacg aagccttcgc
     6721 tctccggccg ggttggcggg agagggtgaa tcgggctctg ctctaggttc tgccgcgctt
     6781 cacacctcgc ccgcgccgct aagaagcagc tgggagaggg atagaaggga gagcaggcga
     6841 accacgagtt cggtcatctt actcagccgc gctcctctgt caatcgaagc gcagaaccga
     6901 tgcgcccgcc cgacaccgcc agccgcagca aatcggccca tttgctgttc ggtgcgcgca
     6961 catcgccgct cgacatacct cagcgccttg cgccgcgtca cttccacgcg cttcgcgcgg
     7021 ccgttacgtt cgtcagagtc atgcgcgcgt ctctccgctc ggtcagtttt gccctctcgt
     7081 tctgcttctc cgcgtgcggc ggagacaatc accccggcat ggacaccgcc gccggcgctt
     7141 cgtcgctcgg cggaagcgct ggacagaact cggcgcgggg cggcagcgcc ggttcggtgt
     7201 cgatcgagac cggcgggagc tccggagcga cctccggagg cgcgggcggc tcagcgcaag
     7261 gcactgcagc aagcgccttt cgctacgggt tcaacgctgg ctatccgaac cccaagttca
     7321 atgatcagga tctcgccgcg ctcggcgtgc gagcgggcgc cacgagcgaa cgcgtcagct
     7381 tgccggagca acacctcgag cgctggggtt acgacatcga ggtcgctgac gtcaaagcgt
     7441 accgcaccct cggcatgggc agcctcgtcg gttttctgac gagcccgacg cgcgcgcact
     7501 ccaccgctcc cgccgacgcg agcgacgacc aagtcggtca gtacatcccg aagaacctct
     7561 acgaaccgat cacgacggcg aacggcgaag tcaacccgaa caactactgg gcaagctacg
     7621 tctaccgaac cgtgtccacg tacggctcct ggatcgacac ctgggaaatc tggaacgagc
     7681 ccgattgggt gtcagactgg aaagtcacgg aggcgtgggc cacgcgcgcg ccgagcgccg
     7741 cggatctgcc acgctttcat ggcagcatct tcgactacgt gcgcatgctg cgcgtctcga
     7801 gcgtcgccgc caagctcgcc aaccccaacg cgaagatcgc gaccggcggc atcggctacc
     7861 caaacttcct cgacgccatc gtccgctact ccgacgaacc gaaggcgggc gcaatcgacg
     7921 cggaccaccc cgcgaccggc aaggcctact tcgacgtcgt gagctttcac cattacccga
     7981 tctacacacc gggcaactcg gaggcaggcg tcgacggctt catgaagcag aggcaagcgc
     8041 tcggcgcgct cgtcgacccg tcgcgcgtgc tcggctggga atgcaccgag accggcgcgc
     8101 cgcacttgcc gctcgatgga attccgagcg gcagcgccta cgcgaagaac tatttgctca
     8161 aggtcatggc gctcgctccg agcgcggatc tcggcggcgt ccattggttc gtgctctcgg
     8221 atgccaaagc ggccggccaa agcacggatc cgtacgcctt catggggctc tacgcgccgg
     8281 tcgcgagcct ctcgactccc tccgaagcag agctcagcga gagcggcgtc gcgtacgcca
     8341 ccctcagcca attgctgcgc ggcgctcact acgacggcat cgcgacttcg gcgctcggcc
     8401 tgcccggtac cgcgcgcggc atcgcgtacc gctcggccga tgacgcgcgc atcctcgtgc
     8461 tctgggccat cgctcccgcc gacagcgaga ctgcgagcac cagcgtcgag ctcgcgagtc
     8521 cacgcacctt gcgccagcac gcttgggact tttccaagac gggccagagc cagctgctca
     8581 cgcccgtgaa cggagtcgtc acgctcgcgt tggaagcgac gccgcgaatc ttcgtcgaac
     8641 agtagcaccg gcgctttttc gcagagcagt tcagcgcgcg cgattcctca ggggggagca
     8701 ccgaaaaagg cgcgcaacca aggcgcagaa cccgatagcg caggcgctca ccggcgccgc
     8761 gcacgctgcc accgcgcccg ccccactccg aaaagccgcg ccggacactc cgcgcgaagg
     8821 cggagcgcac cgccacgctg ccgcggcggc gcgctccgca actcgctaca tcgtggttcc
     8881 accgtcggcg cagaaggcga tctcacattt tcccgcaacg caggcatggt gggcacagcc
     8941 atacgtgccg tccgcgcact gcttacagaa ctggggcagg aggccgtgac agtccgccgc
     9001 ggtacgacaa cccgagacgc actggcaagc cgccgcatcc cagcgctggc cgctcgggca
     9061 cttgatcgga tcgcacaccg cacacacgcc cggcaagtcg ggaatgtgat tgagcacgca
     9121 cttggttccg gctctgcaca cgcagggatt ctgcgtgaat ccgccgcacg gcccaccagg
     9181 acccgacacg cattggtcgg gcacgcactg gcacgccacc gggtcccaat ggctgccttg
     9241 gatacagagc acgttttgaa cacagacctt cgtgaccgcg tctcgttcaa cggcagaacc
     9301 ttcccccggt tcagttcccg cagagcaagc cgagccgagg acgatagcaa acgcgaacgc
     9361 agcgagatgc ttcagtgcca tagaggtact cctcgtcgcg cgttgggttg gcgaagcgcg
     9421 acgaggctga atctatcatg cgcttggtgc gcgcagcgct cgtcacgctc tgtcgcagac
     9481 tcgtcacgaa cgcgttcgca gaactgcgac gcgtgcactg cgaatctcgc aaagtatcac
     9541 ctacgagcga gtgaaagttt atggcaccac tcgtggcgat taacgccgtt cgttcgcaaa
     9601 tctttcagct cacgaatcgt accgaacatc gcacgctcat tc
//
LOCUS       DASTKS010000791         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1017592, whole genome shotgun sequence.
ACCESSION   DASTKS010000791 DASTKS010000000
VERSION     DASTKS010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017592"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFQ35_16370"
     CDS             <1..693
                     /locus_tag="VFQ35_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932281.1"
                     /translation="VVSVDSNGDITVNVATGDQETLGASPTTKNYVWSLTEKTTSNRT
                     VVSTKANWYLPFTNGERTVGPMSLFNSQLFFTSFAPPAVNAHACASGSSKVWGMDYLK
                     PVNYKAASPPKNQGGDGRLPDSTSPTGYTQSYTAQQVTKSSDAVIFGVSVAQQPSCSQ
                     VSDGVKNDFLGYGSQKTLTSVTPGQFQLVMHVGGIKNTTATGDQQSNVRTVDLQPPPS
                     AARVDSWAALVE"
     gene            690..1217
                     /locus_tag="VFQ35_16375"
     CDS             690..1217
                     /locus_tag="VFQ35_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932282.1"
                     /translation="MRFGALCASAAALLGASSCKHAAESRRAEKPLPSSSAAPPVDRL
                     AKGELPPGREALFGLVIPRGMTVQGQFKDSALAFGDMDSEAVANYVRDRVAVSHVEVG
                     AARTIFPAARILEGPPSRVFRIEVVGEGAGTRLLVEDVTPPPPPPPENISDTERWRRA
                     GFTRDGKPLDMNALK"
     gene            complement(1223..1990)
                     /locus_tag="VFQ35_16380"
     CDS             complement(1223..1990)
                     /locus_tag="VFQ35_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932283.1"
                     /translation="MPKGRVLIIESDEWASTLLAKFLREAGYEVEVEEQARSGFDRVR
                     ALEPDCIVCGVALPDIDGFWVARRVRAEQGPVGATPFLFLIDEQDLAGRLHGLNVGGD
                     LFLTKPFRNEVAVAQVGALIGMAKRLKATLPPATEGPPSSGGSTVLQGDLAEMSVSTV
                     LTLLELERRTGRLRARSSDGRTALFDLVDGTLARAELDDATADPLLAMRALLGFTAGT
                     FLFRARPVESTAAPQNLSALMLEALRIEDESKRSHDA"
     gene            2100..3206
                     /gene="gcvT"
                     /locus_tag="VFQ35_16385"
     CDS             2100..3206
                     /gene="gcvT"
                     /locus_tag="VFQ35_16385"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9932284.1"
                     /translation="MTSSLRHTPVHSEHVRLGARLVPFAGYEMPIQYAGISEEHNAVR
                     NKAGLFDVSHMGEIQVSGPAALATVDRIITGDLTRVADGQALYTCACNEGGTILDDLI
                     VYRHGSSDVLVVCNASNREKMVRHFSSFATPEARIEDVSENYALLALQGPRAFDVLAT
                     LRGNGELASSLKSFYFTRTTLGSIEVTIARTGYTGEDGVELFVAPGKAAELFRALLDA
                     GASHGIQPVGLGARDTLRLEARLSLYGNDIDETTNPLEAGLGWIVKLDKPDFVGKAAL
                     VAVRDQGSGRKLVGFEMTGRGIARHGYPLQDSDGRVIGVCTSGGPSPTLGKNIGLGYV
                     ETRFAEVGNVIQVDCRGKSVSAVVVRTPFYRRPR"
     gene            3218..3622
                     /gene="gcvH"
                     /locus_tag="VFQ35_16390"
     CDS             3218..3622
                     /gene="gcvH"
                     /locus_tag="VFQ35_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9932285.1"
                     /translation="MSAATDVKSDRKYTKDHEWALTQGGEILVGITAFAVDQLGDITL
                     VNVDVKPGDKVEAKKTFGTVESVKTLSDLYCPVSGTVVRVNGELEHKPELLNEDPWQK
                     GWMIAIAPSDPSAVAELLDAAAYGNLIASAEH"
     gene            3622..>4160
                     /locus_tag="VFQ35_16395"
     CDS             3622..>4160
                     /locus_tag="VFQ35_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HET9932286.1"
                     /translation="MRYLPHTPEEIQEMLRVVGRASLDELFETVPPAARFKGELAVAP
                     SLDESRLMAHLRGLADKNSGARYLSFLGAGMYAHHIPPAVDQLLLRSEFYTAYTPYQP
                     EVSQGTLQAIWEYQTIVSELYGLPLANASLYDGASAAAEAALMACRLNKRSRVVVSGC
                     VHPDYRATVRTYLSGREGN"
BASE COUNT          766 a         1410 c         1328 g          656 t
ORIGIN      
        1 gtcgtatcgg tcgactcgaa cggcgacatc accgtgaacg tcgcgaccgg cgaccaagag
       61 acgctggggg catcgccgac gaccaagaac tacgtctggt cgctcaccga aaagacgacc
      121 agcaaccgca ccgtggtctc gaccaaggca aattggtacc tgccgttcac caacggcgag
      181 cgcaccgtcg ggccgatgtc gctcttcaac tcgcagctct tcttcacgag ctttgcgccc
      241 cccgcggtca acgcgcacgc ctgcgcgagc ggcagtagca aggtctgggg catggattac
      301 ttgaagccag tcaactacaa ggccgcctct ccgccgaaga atcagggcgg cgatggccgc
      361 ctgcccgact ccacgtcgcc tactggctac acccagtcgt ataccgcgca gcaggtcacg
      421 aagagcagcg acgccgtcat cttcggcgtg tccgtcgcgc agcaaccctc ctgcagccag
      481 gtctccgacg gcgtcaaaaa cgacttcctg ggttacggct cgcagaagac gctcacgagc
      541 gtgacccccg gacagtttca gctcgtcatg cacgtcggcg gcatcaagaa cacgaccgcc
      601 accggcgacc agcagtcgaa cgttcgcacc gttgatcttc agccgccgcc gagcgcggcc
      661 agggtggact cgtgggccgc gctcgtagag tgaggttcgg cgcgctgtgc gcgtcggcgg
      721 cggcgctgct cggagcgtcg agctgcaagc acgccgccga gagtcgtcgc gccgaaaagc
      781 cgctcccgag ctcgagcgcg gcgccgccgg tggatcgcct cgcgaagggc gagcttcctc
      841 ccgggcgcga ggcgctcttc ggcctcgtga taccgcgagg catgaccgtg caagggcagt
      901 tcaaagactc ggcccttgcg ttcggcgaca tggactcgga agccgtcgcg aactacgtcc
      961 gcgatcgcgt ggccgtgtct cacgtcgaag tgggcgccgc gcgcaccata ttccctgccg
     1021 cgcgcatctt ggaagggcct cccagtcgcg tctttcgcat cgaggtcgtg ggtgaaggcg
     1081 cggggacacg cctcttggta gaagacgtga cgccaccacc gccgcccccg ccggaaaaca
     1141 tctccgacac cgagcgctgg cgccgcgccg gcttcacgcg cgacggcaag ccgctcgaca
     1201 tgaacgctct gaagtagcag tctcaggcgt cgtgcgagcg cttcgactcg tcttcgatgc
     1261 gcagcgcttc gagcatgagc gcgctcaggt tctgtggggc cgcggtcgac tcgacgggcc
     1321 gcgcgcggaa caagaacgtc cccgcggtaa aaccgaggag cgcgcgcatc gcgagcaaag
     1381 gatcggctgt agcgtcgtcg agttccgctc gagccagggt accgtcgacc aagtcgaaca
     1441 gcgccgttcg cccatcgctc gaccgggcac ggagccgccc tgttcgccgc tcgagctcga
     1501 gcagagtcag aacggtggaa acagacatct cggcgaggtc gccctggagc acggtcgaac
     1561 ctccgctcga gggcggacct tcggtcgcgg gcggcagtgt cgctttcagc cgcttggcca
     1621 tgccgatgag agccccgacc tgcgccaccg ccacctcgtt tcggaacggc ttcgtcaaga
     1681 acaggtcgcc cccgacattc aggccgtgca gacggcccgc gagatcttgc tcgtcaatca
     1741 agaacaagaa gggggtcgca ccgacgggcc cctgctcggc tcgcacgcga cgcgccaccc
     1801 agaacccgtc gatgtcgggg agagccaccc cgcacacgat gcaatcgggc tcgagcgccc
     1861 tcactctgtc gaagccgctg cgagcctgct cttcgacctc gacctcgtaa cctgcctccc
     1921 gcaggaactt ggcgaggagc gtggaagccc actcgtcgct ttcgatgatg agaactctgc
     1981 ctttcggcat cgtactacgc tagcacgacc ccgaccagcg cagaaaagcc cggaccgctt
     2041 tcccggcgcc gccgcgcgca ggggttgtcg agcggccgac ttcgagctac cgagcggctg
     2101 tgactagctc gctgcgacat acccccgtcc actccgagca cgtccgcctc ggcgcgcgac
     2161 tcgtgccatt cgccggatac gagatgccga ttcagtacgc cggcatcagc gaagagcaca
     2221 acgcggtgcg aaacaaggcg ggcctgttcg atgtctcgca catgggcgaa atccaggtga
     2281 gcggccctgc ggcgctcgcc acggtcgacc gaatcatcac cggcgatctc acgcgcgtgg
     2341 cggatggcca agccctctac acgtgcgctt gcaacgaggg cgggacgata ctcgacgacc
     2401 tcatcgtcta ccgccacggc tcgagcgacg tgctcgtcgt gtgcaatgcc tcgaatcgcg
     2461 aaaagatggt tcggcacttc tcgagcttcg cgacgccgga ggcgcgcatc gaggacgtaa
     2521 gtgagaacta cgctttgctc gccttgcagg ggccgcgcgc cttcgatgtg ctcgccacgc
     2581 tgcgcggcaa cggggagctc gcgagctcgc tcaagtcttt ttacttcacg cgcacgaccc
     2641 tcggttccat cgaggtcacg atcgcgcgca ctgggtatac gggcgaggac ggcgtcgagc
     2701 tgttcgtggc gcccggcaag gcggcggagc tctttcgggc cctgctcgac gcgggcgcta
     2761 gccacggcat tcaaccggtt gggctcggcg cgcgcgacac cctgcggctc gaagcgcggc
     2821 tctcccttta cggcaacgac atcgacgaga cgaccaaccc gctcgaggca ggcctcggct
     2881 ggatcgtgaa gctcgacaag ccggacttcg tgggcaaagc agctctcgtg gccgtgcgcg
     2941 accaaggctc gggccgcaag ctcgtcggct tcgagatgac gggacgcggc atcgcccgtc
     3001 atggataccc ccttcaagac tccgacggac gggtgatcgg cgtgtgcacg tccggcggcc
     3061 cctccccgac cctcggcaag aacatcgggc tcggctacgt cgagactcgg ttcgccgaag
     3121 tggggaacgt catccaagtc gattgccgcg gaaaatccgt ctccgccgtg gtcgtccgca
     3181 caccgtttta ccgacgccca cgttgaaagg aacagccatg agcgcagcga cagacgtcaa
     3241 gagcgaccga aagtacacga aagatcacga gtgggctctc acgcaaggcg gggagatcct
     3301 ggtggggatc accgcattcg cggtcgatca actcggtgac atcaccctcg tcaacgtgga
     3361 cgtgaagccg ggcgacaagg tcgaagcgaa gaagacgttc ggtacggtcg agagcgtcaa
     3421 gacgttgagc gatctgtact gcccggtgag cggcaccgtg gtgcgcgtca acggcgagct
     3481 cgagcacaag cccgagctct tgaacgaaga cccctggcaa aagggctgga tgatcgcgat
     3541 tgctccgagc gatccgagcg ccgtagccga gttgctcgac gccgccgctt acggcaacct
     3601 catcgcgagc gccgaacact gatgcgttac ctgccgcaca ccccggaaga aatacaagaa
     3661 atgctgcgcg tggtcgggcg cgcttcgctc gacgagttgt tcgagacggt gccacccgcc
     3721 gcgcgcttca aaggcgaact cgccgtcgcg ccgagcctcg acgagagccg cctgatggcg
     3781 cacttgcgtg ggctcgccga caagaacagc ggcgcgcgct acttgagctt tctcggggcc
     3841 ggcatgtacg cgcatcacat tccgcctgcg gtcgatcagc tgcttttgcg cagcgagttc
     3901 tacaccgctt acaccccgta tcagccggag gtgtcacaag gcactttgca ggcgatctgg
     3961 gagtaccaga ccatcgtcag cgagctttat gggctgccgc tcgcgaacgc cagcctctac
     4021 gacggtgcga gcgccgccgc agaggcggcg ctgatggcgt gccggctcaa caagcgctcg
     4081 cgcgtggtcg tgagcgggtg cgtgcacccg gactatcgcg ccaccgtccg cacgtatctg
     4141 agcgggcgcg agggtaacag
//
LOCUS       DASTKS010000792         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1912668, whole genome shotgun sequence.
ACCESSION   DASTKS010000792 DASTKS010000000
VERSION     DASTKS010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912668"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..2072)
                     /locus_tag="VFQ35_16400"
     CDS             complement(99..2072)
                     /locus_tag="VFQ35_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9932287.1"
                     /translation="MNDLYEAKLRVGELERQVTELNAQLDERRANRYSTDPRLNRLLG
                     DPLVLATFPHLILVLSRDLRILYINRAESGLDPRDFVGVDCLASMHPEDRERYRVAFD
                     ECWRSGEPASIEVRAASGSWWDSRLVPVRDGGEVAFMLVSSTDVTPRKAQEQTLRDRE
                     SSARLSLLASGVGTWTWWKHKNELYWDEALCAIFGVEPHNAPRSLEEYLALIHPEDRA
                     DAQETIERYVKSGVYEGMAYRIVRPDGKVRHVIAKGIVQLDEHGQVEALRGGVLDVTE
                     RKELEAGLAQAQRMQAVGRLTAGIAHNLNNALSVILPNVAECRELAAEPIAERLADVE
                     HASMRAAEMVRQLMLFARPQENATRSAFDLVAAARRIVDMCRSTFDRKIEIELVTAEV
                     PPVLGNSGQVEQVLLNVCLNARDALLESDRGAPKLRVEFSVPRSGRVAVSIIDNGVGM
                     TEAVRARMFEPFFTTKEVGRGTGLGLSSAYAIVTDHGGTIHCSTRVGQGTRFDIELPE
                     APAAPPAPSLSERVPVPVGTETILMADDEAAVRRVLRRMLERSGFRVIECEDGVQALA
                     ALERGAERVDAILIDRSMPGLSGEEVIARIAQRGIRLPILVLSGHSSVELATPNVVAV
                     LSKPITREALIYEIRRALDQYGAKREERRRDII"
BASE COUNT          434 a          822 c          740 g          411 t
ORIGIN      
        1 gctcggcatg cagctcggga ggttggaaga agcaacgctg ctcttggaag cgcaaaacag
       61 caccggaacg atcgacttca cgagcgccac cgtcgtggtc aaatgatgtc tcggcggcgc
      121 tcctcgcgtt tggcaccata ttgatcgagc gcgcgacgga tttcgtaaat caacgcctcg
      181 cgcgtgattg gcttggacag caccgcgacg acgttcgggg tggcgagctc cacgctcgag
      241 tgcccgctca gaaccaagat cggcagccga atgcctcgct gcgcgatacg cgcgatcacc
      301 tcctcacccg agaggccggg catggagcga tcgatcaaga tcgcgtccac tcgctcggcc
      361 cctcgctcga gggcggctag cgcctgaacg ccgtcctcgc attcgattac tcgaaagccg
      421 ctacgctcga gcatccgacg gagaacgcgt cgcaccgccg cttcgtcgtc cgccatcagg
      481 atggtctcgg tgccgacggg cacgggaacg cgctcggaaa gcgatggcgc aggcggcgcc
      541 gcgggcgcct cgggcagctc gatgtcgaac cgcgtgccct gtcccacgcg cgtgctgcaa
      601 tgaatggtgc cgccgtgatc cgtcacgatc gcgtaggcgc tcgacagccc gagcccagtg
      661 ccgcgaccaa cctccttcgt ggtgaagaac ggctcgaaca tgcgtgcgcg gaccgcctcc
      721 gtcatcccga ccccattgtc gatgatgctg accgcaacgc gtccgctcct cggcacgctg
      781 aactcgaccc gcaatttcgg cgcgccgcgg tcgctctcca agagagcgtc gcgcgcgttc
      841 aagcagacgt tgagcagcac ttgttcgacc tgccccgaat tgccgagcac tggagggacc
      901 tcggccgtta cgagctcgat ctcgatcttt cggtcgaacg tcgagcggca catgtcgacg
      961 attcggcgcg ccgcggcgac gaggtcgaac gcgctccgcg tcgcattctc ttgcggacgc
     1021 gcgaacaaca tgagctggcg caccatctcc gccgcgcgca tcgacgcgtg ctcgacgtct
     1081 gcgaggcgct ccgcgatggg ctcggcggct agctcgcggc actcggccac gttgggcagt
     1141 atcacgctga gcgcgttgtt gaggttgtgg gcgatgccag cggtcaagcg ccccaccgcc
     1201 tgcatgcgct gcgcctgcgc caagcccgcc tcgagctcct tgcgctcggt gacgtcgagc
     1261 acgccaccgc gcagcgcttc gacttggccg tgctcgtcga gctgaacgat gcccttggcg
     1321 atgacgtgac gcaccttgcc gtcgggacga acgatgcgat acgccatgcc ttcgtagacc
     1381 ccgctcttca cgtaccgctc gatcgtctcc tgagcgtccg cgcgatcttc ggggtgtatc
     1441 agcgcgaggt actcttccaa gctgcgcggg gcgttgtggg gctccacgcc gaagatcgcg
     1501 cacagcgcct cgtcccaata gagttcgttc ttgtgcttcc accaggtcca ggttccaact
     1561 ccgctcgcga gcaacgacaa ccgcgcgctg ctctcgcggt cgcgcagggt ttgctcttga
     1621 gccttccgcg gcgtgacatc ggtgctcgaa accaacatga acgcgacctc gccgccgtcc
     1681 cggaccggca cgagccgcga gtcccaccag gatccactgg cagcgcgcac ctcgatactg
     1741 gccggctcac cgctgcgcca gcactcgtcg aacgcgacgc ggtaccgctc tcgatcctcc
     1801 gggtgcatcg aagccaaaca atcgacgccg acgaagtctc gtggatccaa gcccgactcc
     1861 gcacgattga tgtacaagat ccgaaggtcg cgactcagca ccaggatgag atggggaaag
     1921 gtcgcgagga ctagcggatc gcccagcagt cgattcagcc gcgggtcggt cgagtagcgg
     1981 ttggcgcggc gttcgtcgag ctgagcgttg agctcggtga cttggcgctc gagctcccca
     2041 actcgaagct tggcctcgta caggtcgttc aactccagct cctaatccaa acgtattcgc
     2101 actcgtggac gattgcacgg cagcaaacgc ccgagatcga ggcgcggcgg tgggccaccc
     2161 gtacgccaag tggggcaacc cgccacagca cggaacgaaa tgctgcaatc cgaccacgcg
     2221 aacgacgctc cctcgacggc gcgcacactc cgccaagccc atgacgacaa tgtgctcaaa
     2281 acggtgctgc catcgtcttt gggcatattt cccatactca aggcgccgta aggatgtact
     2341 gctgccaact cgtacgaaac gccttttcga ggtctcgagc gaagcgcgag gcgtcgccga
     2401 gtggaga
//
LOCUS       DASTKS010000793         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_572162, whole genome shotgun sequence.
ACCESSION   DASTKS010000793 DASTKS010000000
VERSION     DASTKS010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572162"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFQ35_16405"
     CDS             complement(<1..174)
                     /locus_tag="VFQ35_16405"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932288.1"
                     /translation="MITHPEKQLFPSDGISKGELAAYYELVAPHLIPFVRGRPVTLER
                     FPAGIERKGFIQKD"
     gene            620..892
                     /locus_tag="VFQ35_16410"
     CDS             620..892
                     /locus_tag="VFQ35_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932289.1"
                     /translation="MISNISRLIVTLGFVGLNVAACTANVENPTVDQTGRDGDTTCVT
                     DCDDAQTTCVAKCSDDTCKATCKTTHDSCASKCTVTTTTGTGGKSG"
     gene            1168..2031
                     /locus_tag="VFQ35_16415"
     CDS             1168..2031
                     /locus_tag="VFQ35_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932290.1"
                     /translation="MVAALEPAISAPAAAAPAGALDAETAAPRVEAWTEASPEVLSDL
                     RAGRPLVTVLQVPLCSNRQIRCGGQGAGDPASLDKNLYWGRGFGVRRYFDETARGWRL
                     IARSEPGGTTLEQRVYARDFDGSSFGLPSGHTVRHLVVMRAVNGDAIESAVDSFYELA
                     TRGGRVRFEEDGQARDERVHVAGYAGHNRLMDGYRLKEAAVDSASSLPSFVIACRSSQ
                     YFSSALAAHGSTTLVMTRDLVAPEGYVIEALANALGRNSSPREIRAQVVRAYARWQKL
                     SENVASTIFAR"
     gene            2118..2990
                     /locus_tag="VFQ35_16420"
     CDS             2118..2990
                     /locus_tag="VFQ35_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET9932291.1"
                     /translation="MRIATWNVNSLRARIDKVSWWLERARPDVLLMQETKMTDDDAPS
                     DEFARQGYELAHHGEGRWNGVAIASRLPIRDVVTNFGQPLQPPKSPDVGDDEPLAEAR
                     MISAVCGDVRVVSIYAPNGRSLGSEFYKAKLVWFARLLRWFQETRSPNEAVALGGDFN
                     VAPTDQDVWEANACHGGTHVSPEERAAIAALTDWGLADAYRLHHPEPNRYTWWDYRAG
                     AFHKNMGMRIDHLLVSRALAPRVVASEIDREARKGKPTPSDHTPLVIDLDEPGVPFDA
                     GWAGAEGRIAARRV"
     gene            3175..3609
                     /locus_tag="VFQ35_16425"
     CDS             3175..3609
                     /locus_tag="VFQ35_16425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HET9932292.1"
                     /translation="MAIWFDEFPLSFVREHSQNTLIAQLGIEFIEAGDDYLTARMPVD
                     ARTHQPAGMLHGGASVALAETLASWGATFTVDREKNHCVGLEINANHVRAVGSGWVIG
                     AGRPLHIGRSTQIWEVRIVDEQSRLVCVSRVTMAVLEMKNRY"
     gene            complement(3603..4541)
                     /locus_tag="VFQ35_16430"
     CDS             complement(3603..4541)
                     /locus_tag="VFQ35_16430"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9932293.1"
                     /translation="MTTRFGYRKVLVVGAKGTVGSEATRALLERGAAVRALVRSLRGT
                     ERLDACEFVVGDLKDDKATAAALEGVSAAFYVSPHEPDEETLAETFVRACERARVRLV
                     FVGVHADAPTRLQRALKRFMFGRLLSPYAPKFRISERVRRSSADPVVLMPTNYFQNDE
                     LFLDQIAAGHFTQPFERAVNRVDVRDLGAAAARACLDPTLPSGAYPVVGPASLDAAAC
                     AEIWANELGSDVRYVRDESLEALRSALAGKLSGKKRQDFTASYSAIRRYELPTSQAEL
                     ARTAALIEREPTPYAAYVRDAVKRLRAAREANTAAQ"
BASE COUNT          831 a         1514 c         1520 g          779 t
ORIGIN      
        1 gtccttctga atgaatccct ttcgctcgat ccctgccgga aagcgttcga gggtcaccgg
       61 ccggccgcgc acgaatggga ttaggtgggg cgcgacgagc tcgtagtaag cggcgagctc
      121 tcctttgctg atcccgtcac tcgggaacag ctgcttctcc ggatgtgtga tcacgacgtc
      181 caatggcttg ccccgagtct agacgaggcg gcggcccgta gccgcaggat cttgcctcgg
      241 agccggccga gaacttggca atgctggagc gtggcacccg cgcgcggcgc tccagagtga
      301 gaaagcagaa cgccgcgggg cgcgagcacc cgtgctgggg caccagcgca cgcaggtgat
      361 ccagcgaggc agcacataga ctttcgctag cagtcgaccc gctcgggaga gcgccacgct
      421 gcggcgggaa tgaagtactc agaaggggcc ggcggagcgt ggcaggctgt caccttacgc
      481 tcgattccgc tccaaacctg ggcgacattc ggtgtttgcg cagcgtccga gaatgacgca
      541 gtcgctgtac gacttacgtg gcatcccgct tgctcaagta gccatcagaa acggcgatga
      601 gccgcttacg gaggaacaaa tgatttcgaa tatcagtcgc ctcattgtga ccttgggctt
      661 cgtgggtttg aacgtcgctg cgtgcaccgc aaacgttgag aacccgaccg ttgaccaaac
      721 cgggcgtgac ggcgatacca cctgcgtcac cgactgcgac gatgcccaga cgacgtgcgt
      781 cgcgaaatgc agtgacgaca cctgtaaggc cacgtgcaag acgactcacg acagctgtgc
      841 ctccaagtgc accgtgacga ccacgaccgg caccggcggc aagagcggct gattcctctg
      901 ctaagggccc gcgaatgaac aagtcagtcg cgagcgggcc gagaccgcag cgaggcgggc
      961 gcgccctgag ggccttcacc tagcgttagc cttccacggc ttatgcgggg cgagggccct
     1021 cgccaaccta tcttcgcgtg gtgagagcag tgagatgcca aggatagtgg acttggtcgg
     1081 tcacactagt tcgtggaacc ccgatatgag agagtgaagc ccactcgacg cgcgtcttgg
     1141 cctgcactcg gtctcgtcgt tcccctcgtg gtcgcagctc tggagccagc tatctccgct
     1201 ccggctgccg cggctcccgc gggtgccctc gacgctgaaa cggctgcgcc gcgagtcgaa
     1261 gcttggaccg aggcgagccc cgaggtgctc tcggaccttc gcgccggccg tcctctcgta
     1321 acggtactcc aggttccgct ctgctcgaac cgacaaatca gatgcggcgg ccagggtgcc
     1381 ggtgacccag cgtcgctcga caagaatctc tattgggggc gcggcttcgg tgttcggcgc
     1441 tacttcgacg agactgcccg cgggtggaga ctcatcgcgc gctctgagcc aggaggcacg
     1501 acgttggagc agcgggtcta cgcgcgtgac ttcgacggct cgagcttcgg cctgccctcc
     1561 gggcacacgg tgcgacacct cgtcgtgatg cgcgccgtga acggcgacgc gatcgagagc
     1621 gcggtcgatt cgttttacga actcgcgact cgaggagggc gcgtacgatt cgaggaggat
     1681 ggacaagcgc gcgacgagcg ggtccacgtc gcggggtacg cggggcacaa ccgtttgatg
     1741 gatgggtacc gactgaagga ggcggcggtg gactccgcga gctcgctgcc ctcgttcgtc
     1801 attgcgtgcc ggtcctcgca gtacttctcg agcgcgctcg cggcgcacgg ctcgacgacg
     1861 ctggtcatga cgcgcgacct ggtcgcgccc gagggttacg tgatcgaggc gttggcaaac
     1921 gcgctcggac ggaacagctc cccccgggag attcgagctc aggtggtccg cgcgtatgca
     1981 cgctggcaga agttgagcga aaacgtggcg agcaccattt ttgcgcgctg aggccggccc
     2041 tgggctcacc ggcaccggcg atagcagtgg cacaaactcg tagtcgctgc ccctcgcttt
     2101 tgctacaccc gccgaccatg cgaatcgcaa cctggaacgt gaactccctc agggcgcgca
     2161 tcgacaaagt ctcttggtgg ctcgagcgcg cacgcccgga cgtgctgctc atgcaagaga
     2221 ccaagatgac cgacgacgac gcacctagcg acgagttcgc gcgccagggt tacgagctcg
     2281 cacaccacgg cgaaggacgg tggaacggcg tggccatcgc gagccgcttg ccgatcaggg
     2341 acgtggtcac caattttgga cagccgcttc aaccaccgaa gagtccggat gtcggcgacg
     2401 acgagccgct cgccgaggca cgcatgattt ctgccgtctg cggggacgtg cgcgtggtca
     2461 gcatttacgc gcccaacggt cgttcgctgg gttccgagtt ttacaaggcg aagctcgtct
     2521 ggttcgcgcg gctactacgc tggtttcaag agacgcgcag cccgaacgag gcagtggcgc
     2581 tgggcggcga cttcaacgta gcgccgaccg accaagatgt ctgggaagcc aacgcctgtc
     2641 acggcggaac ccacgtctcg cccgaggagc gcgcggcgat cgcggcgctt accgattggg
     2701 gcctcgcaga cgcctatcgc ctgcatcatc ccgaaccgaa ccgttacacg tggtgggact
     2761 accgagccgg tgcctttcac aaaaatatgg gcatgcgcat cgaccacctg ctcgtctcgc
     2821 gcgcacttgc gccgcgcgtc gtagcgtccg aaatcgatcg cgaggcccgc aagggcaagc
     2881 ccacgccgtc ggatcacacg ccgctcgtca tcgatttgga cgagcccggt gtcccgttcg
     2941 acgcgggctg ggccggcgcc gaggggcgaa tcgccgcccg tcgagtctga cgcgcgctgg
     3001 gcaggcccgt ggaccgcgtg ccgcctcggc atcaacgcaa agcggaagca aacgcgggaa
     3061 cgtttccgca tccaagtgga aacgacccta actcgtgtgc agcgagcgga aacgaaaccg
     3121 agtttcaatc gctcgcgacc tcccgatcga caatccgggt gtactctccg tcgcatggcc
     3181 atttggttcg acgaatttcc gctgagcttc gtgcgcgagc acagccagaa caccctcatc
     3241 gcccagctcg gtatcgagtt catcgaagcc ggagacgact acctcacggc gcggatgccg
     3301 gtcgacgcgc gcacccatca gcccgccggc atgctccatg gcggcgcgtc ggtggcgctc
     3361 gctgaaacgc tcgcgtcttg gggcgccacc ttcacggtcg accgggagaa gaaccactgc
     3421 gtcgggctcg agatcaacgc gaaccacgtg cgcgcggtcg gctccggttg ggtcatcggg
     3481 gcgggacggc cattgcacat cggtcgcagc acccaaatct gggaggtgcg catcgtcgac
     3541 gagcagagcc gcttggtatg cgtctcgcgc gtcaccatgg ccgtcctcga gatgaagaac
     3601 cgctactgag cggcagtgtt tgcctccctc gcagcgcgaa ggcgcttcac cgcgtcacgc
     3661 acgtacgccg cgtaaggcgt tggttcgcgc tcgatgaggg ccgcggtgcg ggcgagctcc
     3721 gcttgggagg tcggcagctc ataccgccga atcgcggagt agctcgcggt gaaatcttgg
     3781 cgcttcttcc cgctgagctt tccggcgagc gctgatcgaa gcgcttcgag agactcgtcc
     3841 cgcacgtagc gaacgtcgga cccgagctcg ttcgcccaga tctccgcgca agcggccgcg
     3901 tcgagcgatg cgggacccac taccgggtag gctccggacg gaagcgtcgg gtccaggcag
     3961 gcgcgcgccg ccgcagcgcc caggtcgcga acgtccacgc ggttcaccgc gcgctcgaac
     4021 ggctgggtga agtgtcccgc cgcgatttgg tcgaggaaga gctcgtcatt ttgaaagtag
     4081 ttcgtcggca tgagcaccac cggatccgcg ctcgaacggc gcactcgctc cgagatccga
     4141 aacttcggcg cgtacggcga tagcaaacgg ccaaacatga agcgcttgag cgctcgctga
     4201 aggcgcgtcg gcgcgtcggc gtgaactccc acgaacacga gccgaacgcg cgccctctcg
     4261 cacgcgcgca cgaacgtctc cgcgagcgtt tcctcgtccg gctcgtgcgg agagacgtag
     4321 aaggcagccg aaacgccctc gagggcagcc gcggtggcct tgtcgtcctt gaggtcgccg
     4381 acgacgaatt cgcatgcgtc gagccgttcg gtgccgcgta gcgaacgaac cagggcgcgc
     4441 accgcggccc cgcgctcgag gagagccctc gtcgcctcgc tccccaccgt gcctttcgcc
     4501 cctaccacca ggacttttcg gtatccaaat cgtgtcgtca tcgttttcct aggcggcgtc
     4561 tcgcgcaccg ctcatccctc ccagtgaggc gaagagcggc ttcgttacgg cgtctcattc
     4621 cgaggcggga agcggcgctt ggac
//
LOCUS       DASTKS010000794         8414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1018235, whole genome shotgun sequence.
ACCESSION   DASTKS010000794 DASTKS010000000
VERSION     DASTKS010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018235"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFQ35_16435"
     CDS             complement(<1..551)
                     /locus_tag="VFQ35_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932294.1"
                     /translation="MTGVWFPALLGVSLCAVAFLVAEPARASPSMNLECPELASERAE
                     VEARFLASAVSRDAADLTITIRCRDGIAELEVSVGARDQRRGVSFASEIAREAILTNL
                     DRALSELVPSEATPQSMRASLPEPIARPSAATPARAQAPVIAPTPPPPKPPVAAPCVR
                     NSTPNRVRAEAALESWGSTLALAG"
     gene            652..1251
                     /locus_tag="VFQ35_16440"
     CDS             652..1251
                     /locus_tag="VFQ35_16440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9932295.1"
                     /translation="MTRTQRSSVHRLPQPVVMCASSGGPATERELFELVYRRMRSLAA
                     GTPDFDDLVQAAAEQVFRSLPSFDGRSELETWVYAVCYRVMLAQRRWYWRFKRRFSLD
                     WGEPALELGLRDESPTPDQRIESRQRLEVLNRALARMSDKYRAVLVLYYLEDFETAQI
                     AAIVGSNELTVRSRLRDARKQLLKLLGKDAALICEETAT"
     gene            1248..1907
                     /locus_tag="VFQ35_16445"
     CDS             1248..1907
                     /locus_tag="VFQ35_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932296.1"
                     /translation="MKSFLREDVVTAFRADEPDASTLQRAYVRFSWTRRRSAPPLRLL
                     RWVALGILVGLGGASAATLLPHRLVSGEHPVPVVSVRARAQRHAPLRAERAVAPSPSA
                     PDAHSTARSIASAPLSAPPAAPQPAVAKEPESSNGEWARVAQALRASDFEGARAALSR
                     LKRSANTREREAAELAEAQLLVKAGDRTSPRSTLVRLSRDALSPLVRAQANALLNEVS
                     K"
     gene            1968..2516
                     /locus_tag="VFQ35_16450"
     CDS             1968..2516
                     /locus_tag="VFQ35_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932297.1"
                     /translation="MIQSNLFQRTCFLTALFTFACGGPIDIGKINGYPAGGTEPDAKG
                     GDVGSGGSQSSGGATNSGGATASGGTEQSGGATSAQGGAVPASGGTMGQGGSVPSASG
                     GTGTTPECSQDSDCTMCSTFNDPTEVGCYIPCCETRPKSVAQCSLNWQHLMTQCGGGE
                     CLVLCSQQPPHVACVNGVCTAP"
     gene            complement(2546..3466)
                     /locus_tag="VFQ35_16455"
     CDS             complement(2546..3466)
                     /locus_tag="VFQ35_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932298.1"
                     /translation="MRLEGASSCLDAATLAERVRKRLGNDPFDAQAKRSIEGIVRRAD
                     GVWLAEIAVRKRADDAEPPLRQLKSTAPDCASLGDAIVLAVALAIDPEAAFAATEAEE
                     PPPPPAATRAPVPPPPPALPLPPSMPRVPPQSVRLGSVTASAVFQVGALPRASLGAGL
                     FAAAALGSSVDVGIGARVFPSVRGDGEPGFEVGASVGTLQLCPRLRTSSVTTARVCAG
                     PSLGVMHAVLLTGERAQPGERLWLAAEAGADFTLDVSKSLALMLGAGGLVPITRYRFS
                     VEGTDGTLFRQSALALVAQLGVELRFSGAR"
     gene            complement(3571..4401)
                     /locus_tag="VFQ35_16460"
     CDS             complement(3571..4401)
                     /locus_tag="VFQ35_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932299.1"
                     /translation="MSARSLVIALASVTLAACSIDWRLARGDGGAGSGASGGGGGAPV
                     LANAALLCDRIPSLSTEPVVDGKLESGLSLYTWLDEASRDTRSGVSANVSLAYRPDGV
                     YFFAAVVDPTRDPAPLDALPYCGDGVELYVDDDGSIRNPPGYDRPGTMQLIVAAPSGG
                     VAAEHRGERFVNPGNGDGIDLGAWSSSRFAAVPTADGYVVEAFVVATDLDLASWRLAP
                     GAQIGWNLSLNIGGPEPAGIDACTTRSQQIHFRLATSDACTAPFCNASAFCATALLGP
                     "
     gene            complement(4398..5090)
                     /locus_tag="VFQ35_16465"
     CDS             complement(4398..5090)
                     /locus_tag="VFQ35_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932300.1"
                     /translation="MNDPIRIRESGSDAPDELRELFRSAKGPEPLTAPVEAALSTRVA
                     GISSARSAALAKALPYLVSAALAGGGLVAWRATTQRSAHPASVAIAPSSTSSSLPAVA
                     PAPSASSVGVASPESVVAPSDASARLRVRAQSSAAPAKSADEDSLSGESRLLNQAHRA
                     LTTDPARALAIAREHAKRYPHGQLAAERELIMVQALVKLGRTREAEARGQELRKTAPK
                     SIYEERLDKVLR"
     gene            complement(5087..5662)
                     /locus_tag="VFQ35_16470"
     CDS             complement(5087..5662)
                     /locus_tag="VFQ35_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932301.1"
                     /translation="MSDANAVPRTTQATASPLDVGAVYASQRDFVWLTLQRMGVRRAD
                     LEDVFQDVFMIVHRRLHTYRADAKLSAWLYGICLRCVARHRRRAFRRRESPEGVELEQ
                     RDSSPWQARVDAPDRHLDDAQRQERLNRLLDVLDPEHRAMVVMYEIEELGCAEISELT
                     GVKLGTIHSRLHAARRKLVSAARRFKGEEGR"
     gene            5883..7802
                     /locus_tag="VFQ35_16475"
     CDS             5883..7802
                     /locus_tag="VFQ35_16475"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9932302.1"
                     /translation="MLRPFVVAIVCLTPACTTLFGDRNPGGEDETPDATHSGGTSGSN
                     ATGLEGGASTANGAAPGHGGTGGAASGSGGTNGGGKPGSGGGAGLDSSQPAGPQFTCN
                     SANGSAELPFPRLSRAQLQNTLKFAIRSALPADADRIWNSVSTRFAEYPVDQRTAAPG
                     DLKGGYSRFDQSIQLAQVQAMYGLGIEIARELTSTTARLTSLLGACATDASTANDRAC
                     LETFLRRWAARVMRTELSSEDVAFYADIAGTTPVAAVAVADVVAAVLNAPETLYRVEH
                     GTGTSSPSPLSSFELAARLSFNLWQEPPDDELWAAASNGSLLQASTYESQLARLVSDP
                     KLRRSLDEWVSEWLRLGELPQLDTLKDDPAFAAFAGSDLPTQSTRQAMIDDVLESAWS
                     TTRSGQPASALLADRHAYAKDPILARIYGVTQWDGVGEAPLFPSPNRSGLLTRAALLA
                     TGTAGTRPIHKGYLVRNALLCQQLGAPPLNANTEVPVATGQLTTREAVSNRTSPASCT
                     GCHSIINPAGFITEKFDALGRERDAERLFDARGQLIAALALDTAAAPGVIPGDTRVMR
                     SAPELTEAIDTSRLFHSCVARHYFRFASARVEDTERDGCLLAKLEEVARSGASLKALL
                     QAFAQDPTFKNKRFE"
     gene            7799..>8414
                     /locus_tag="VFQ35_16480"
     CDS             7799..>8414
                     /locus_tag="VFQ35_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932303.1"
                     /translation="MSNGSHLLRINRRFFLRGVGGTLLALPTLPSLLRPKTARAEAAT
                     PPKCFVHFRTPHGGISTANMWPNEATLTDTLGYTHDIRRGALKATIDSGNSVVSPVLT
                     ASSSVLTASLVAKMNVLRGLDIPIAMAHNFGAALGYYDVDHQTPARPTATVDQVLAYS
                     SAFYPELGSVKQRSVVISAPGTNSGSHGYVTPGVRTSGVSKDALS"
BASE COUNT         1488 a         2814 c         2774 g         1338 t
ORIGIN      
        1 ccggcgagtg ccaatgtcga cccccagctt tcgagtgcag cctcggcgcg aacccgattg
       61 ggcgtcgagt ttcgaacgca aggcgcggcg acaggtggct tcggtggcgg cggagtcggc
      121 gcaatgaccg gagcctgcgc ccgcgcggga gtcgcagcgg agggcctcgc aatcggttcc
      181 gggagtgaag ctcgcatcga ttgcggggtc gcttcactcg gaacgagctc gctcaaggct
      241 cgatcgaggt tggtgagtat cgcctcgcga gcgatctcgc tcgcaaaaga caccccgcgc
      301 cgctggtcac gcgcccccac cgacacttcg agctcggcga tgccgtctcg gcagcgaatc
      361 gtaatcgtaa ggtccgccgc gtctcggctc accgcgctcg ccaagaatcg agcctcgacc
      421 tcggctcgct cggatgcgag ctccgggcat tcgaggttca tggaaggact ggctcgtgca
      481 ggctccgcga ccaagaaagc aactgcacag agactcaccc cgagcaatgc tgggaaccaa
      541 accccggtca cgggggctag cctacgcgaa actggaaaga ttcgtcgacc tcgtcgctcg
      601 tgaattttgc accgctcgtt cgaaccggga gcggcaacca acctacgatc cttgactcga
      661 acccagcgct ccagcgtgca ccgtttaccg cagccggtgg tgatgtgtgc ctcgagcggc
      721 ggccccgcga ccgaacggga gctcttcgag ctcgtgtatc gacgtatgcg gagcctggcg
      781 gcgggcacac ctgacttcga cgacttggtc caagcggcag cagagcaggt gtttcggagc
      841 ctgccttcgt tcgacggccg tagtgagctc gagacgtggg tatacgcagt ctgctatcgc
      901 gtgatgctgg cgcaacggcg ctggtattgg cgcttcaagc gccggttcag cctcgactgg
      961 ggagagcccg cgttggagct tgggttgcga gacgagagcc cgacacccga tcagcgcatc
     1021 gagtcgcgcc aacggctgga ggtcttgaac cgcgccttgg cgcgcatgag cgacaagtac
     1081 cgcgccgtgt tggtgctcta ctacttggag gacttcgaga ccgcgcagat tgccgccatc
     1141 gtaggcagca acgagctgac ggttcggtcg cggctgcgcg acgcgcgcaa acagctattg
     1201 aagttgttgg gcaaggacgc ggctctgatt tgtgaggaga cagcgacatg aagtcgttct
     1261 tgcgtgagga cgtggtgacg gcgttccgtg cggacgagcc ggacgcttcc acattgcagc
     1321 gcgcctatgt ccgcttttct tggacgcgac gtcgaagcgc accgccgctg cggctgctcc
     1381 gctgggtagc actggggatc ctggtcgggc tcggcggcgc gtcggcggcg acgctgttgc
     1441 cccaccgcct ggtttctgga gagcatcccg tgccggtcgt cagtgtacgg gcgcgcgcgc
     1501 aacgtcacgc gccccttcgc gcggagagag cggtagcgcc ctcgcccagt gcgcccgatg
     1561 ctcacagcac cgcacggagc atcgcgagtg cgccactcag cgcgccaccc gcggcgccgc
     1621 aacccgcggt ggcgaaggag cccgagagct cgaacggcga gtgggctcga gtggcgcaag
     1681 cgctgcgagc gtcggatttc gaaggggcgc gcgccgcgct ctctcgcctg aagcgcagcg
     1741 cgaatacgcg cgagcgggaa gccgcggagc tcgcggaggc gcagctactc gtaaaggctg
     1801 gcgaccgaac gagcccgcgc tcgactctcg ttcggctctc gcgcgatgcg ctctcaccat
     1861 tggttcgcgc gcaagcaaac gccctgctga acgaagtgag caagtgaaat gaagagcact
     1921 cgcagcttcg ggcgctcgcc gagccgtcga ccggcaacga agaagacatg attcaatcga
     1981 acttgtttca gaggacttgc ttcttgacgg cgctcttcac attcgcgtgc ggcgggccaa
     2041 tcgatatcgg caagatcaat ggctacccgg cgggtggcac ggagcccgac gcgaagggag
     2101 gcgacgttgg gtcgggaggt tcgcagagca gtgggggcgc gacgaacagt ggaggtgcga
     2161 ccgcatcggg cggcacggag caatccggtg gcgcaacttc ggcgcagggg ggcgcggtac
     2221 cggcgtcggg cggcacgatg ggccagggcg gttccgtgcc ttcggcgagc ggcggaacgg
     2281 gaacgactcc cgagtgctcg caagactcgg actgcaccat gtgttcgacg ttcaacgacc
     2341 cgacggaggt tggttgttac attccatgtt gtgaaaccag gccaaagtcc gtggcgcagt
     2401 gctcattgaa ctggcagcac ctcatgaccc agtgcggagg cggtgaatgt ttggtgctgt
     2461 gtagccagca accacctcac gtggcgtgcg tgaacggtgt ttgcacggcc ccatagcgcg
     2521 cgggcttcgg cccaacgccg cgatctcacc ttgccccgct gaagcgaagc tcgacgccaa
     2581 gttgtgcgac gagagcgagc gcggactgac gaaacagcgt tccgtcagtc ccctcgacgc
     2641 taaaacggta gcgcgtgatc ggtacgaggc cgccggctcc gagcatcagg gcaagtgact
     2701 tcgaaacatc caaggtgaaa tcagcgcctg cctcggccgc gagccacagg cgctcaccag
     2761 gctgcgcccg ctctccggtg aggagcacgg cgtgcatgac accgagcgac ggtccggcac
     2821 aaacgcgtgc ggtcgtaact gagctggttc gcaagcgcgg acagagctgc aatgtgccca
     2881 cgctcgcacc gacttcgaaa ccgggctcac catcgccgcg cacggacgga aaaacgcgcg
     2941 cgccgatgcc aacgtcgacg gatgacccga gcgccgccgc ggcgaacagc cccgcgccaa
     3001 gcgacgcacg cggcaacgcg cccacttgga atacagcgct cgcggtgacg ctgccgagac
     3061 gaacactctg gggcggaacc cgaggcatcg atggcgggag gggcagggct gggggcggcg
     3121 gcggcaccgg agctcgagtg gcggcgggag gcggtggcgg ctcttccgct tcggtggccg
     3181 cgaatgccgc ctctggatcg attgcaagcg ccaccgccag tacgatcgcg tcgccgagcg
     3241 acgcgcaatc tggagcggtg ctcttcagct ggcgcagcgg cggctcggcg tcgtcggcgc
     3301 gcttccgcac cgcgatctct gcgagccaaa cgccgtccgc gcggcgaacg atcccttcga
     3361 tgctgcgctt cgcctgcgca tcgaacgggt cgttgcccaa gcgcttgcga acccgctcgg
     3421 cgagcgtcgc tgcgtcgagg caactactag ctccttcgag ccgcacccat tgcaggcgga
     3481 ccggctcgga agcattcgcg gagcgtgagt agctcccaag cagacagcat gcggcaatac
     3541 cgagccgcag cgcacgaaag gagcggaaag tcaggggccc agcagcgcgg tcgcgcaaaa
     3601 cgcggaggcg ttgcaaaaag gggcggtaca tgcgtcggaa gtcgcgaggc ggaagtggat
     3661 ttgctgactg cgagtcgtgc aggcgtcgat gcccgctggt tccggcccgc caatgttcag
     3721 gctcaagttc cagccgatct gcgcgcccgg cgcaagcctc cagctcgcca ggtcgaggtc
     3781 ggtcgccacc acgaacgctt cgacgacgta gccgtcggcg gtaggaaccg ccgcgaaacg
     3841 tgacgaggac caagccccga ggtcgatgcc atcgccgtta ccggggttga cgaagcgttc
     3901 ccctcgatgc tccgccgcca cgccaccact cggcgccgca acgatgagct gcatcgtgcc
     3961 gggcctatcg tatcccggag gattgcgaat gcttccgtca tcgtcgacgt acagttcgac
     4021 cccgtccccg caataaggca gcgcgtcgag cggcgcgggg tcacgcgtcg gatccacgac
     4081 cgcggcgaag aagtatacgc catccggacg ataagcgagg ctgacgttgg cgctaacccc
     4141 ggaacgcgta tcccgacttg cttcgtcgag ccacgtgtac agcgacaggc ccgattcgag
     4201 ctttccgtcc accaccggtt cggtcgaaag agaagggatt cggtcgcaga gcagcgccgc
     4261 gttggcaagg actggcgcgc cgccaccgcc accgcttgcg cccgaaccag ctcccccgtc
     4321 tccgcgggcg aggcgccaat cgatcgaaca cgcggcgagc gtcacggacg cgagcgcaat
     4381 gacgaggctc cgcgcgctca acgcagtacc ttgtccaagc gctcttcata gatgctcttc
     4441 ggcgccgtct tgcgcagctc ttggccgcgt gcttccgcct cacgcgtccg gcccagcttg
     4501 accagcgctt gcaccatgat gagctcacgt tcggccgcca gttgcccatg cggatagcgt
     4561 ttggcgtgct cacgagcaat cgctagcgcc cgagcggggt cggtggtgag agcgcgatgc
     4621 gcctggttca ggaggcgcga ttcgcccgaa agcgagtcct cgtcggccga cttggcggga
     4681 gccgcagagg attgagcgcg gacgcgaagg cgcgcgctcg cgtcgctcgg agcgaccacg
     4741 ctctcgggcg aggcgacacc aacggacgaa gcgctcgggg ccggcgccac ggcgggcaat
     4801 gatgaagaag tcgagctcgg agcaattgcc accgatgccg ggtgcgcgct ccgttgcgtc
     4861 gtcgcgcgcc aagcaacgag cccgccgccg gcgagcgccg cgctgacgag gtacggcaac
     4921 gccttcgcga gcgccgcact gcgcgcggaa gagatcccgg cgacgcgggt cgacaacgcc
     4981 gcctcgaccg gcgccgtcag cggctcaggc cctttggcgg accgaaacag ttcacgcagc
     5041 tcgtccggcg catcggagcc cgactcgcga atgcgaatgg gatcgttcat cgtccctcct
     5101 cccctttgaa gcgccgcgcc gcagacacga gcttgcgacg ggcggcgtgc aggcgcgagt
     5161 gaatggtccc gagcttgacg ccggtgagct ccgagatctc cgcgcagccg agctcctcga
     5221 tttcgtacat gacgaccatc gcgcggtgtt cgggatcgag cacatcgagc aggcgattca
     5281 atcgctcttg cctttgcgcg tcgtccaaat gccgatccgg cgcgtccacg cgcgcttgcc
     5341 aggggctcga atcgcgctgc tcgagctcga ccccctcggg gctttcgcgc cgccgaaacg
     5401 cccgccgccg gtgccgcgcg acgcaacgca ggcaaattcc gtagagccac gccgaaagct
     5461 tcgcgtccgc tcgatacgtg tgcaggcggc gatgcacgat catgaatacg tcctggaaca
     5521 cgtcttccag atccgcgcgg cgcacgccca tgcgttgcaa agtgagccac acgaagtcgc
     5581 gctggctcgc gtagacggct cccacgtcga gggggctcgc cgtcgcctga gtcgttcgcg
     5641 gaactgcgtt cgcgtcactc aatgctgatg tccgttcgcg ggtcaacgct ttaaccaatt
     5701 tgacggaggt ctgccaatcg acgctcactc gttcatgaca tctcgccgag ggcgcggccg
     5761 actgcccatt ctacacgctg acgtaaggga aggctcgaac cgagtggccg gcatcgtcca
     5821 cgcgacgtgg gtacgatctt tttttcgaag cgaagcgcgc cgccccggca ttacccacga
     5881 agatgctgcg ccccttcgtt gtcgcaatcg tctgtttgac tccggcctgc acgacattgt
     5941 tcggcgaccg aaacccggga ggcgaggatg agacgccaga cgcaacccac agtggcggca
     6001 cgagtgggag caacgccacg gggctcgaag gcggcgcctc caccgcgaac ggagcggcgc
     6061 cgggacacgg cggcacgggc ggagctgcgt cgggaagcgg cggcacgaac ggtgggggaa
     6121 aaccggggag cggtggcgga gccgggctcg actcgagtca gcccgcgggt cctcagttca
     6181 cctgcaactc ggcgaatggc agcgccgaac tgccgttccc acggctctcc cgcgcgcagc
     6241 tgcaaaacac gttgaagttc gccatccgct cggccctgcc cgcggacgcg gaccgaattt
     6301 ggaactccgt gagcacgcgc ttcgcggagt atccggtcga tcaacgaacg gccgcaccgg
     6361 gcgatctgaa aggcggctat tcgcgcttcg atcagtcgat tcagctcgcg caagtgcagg
     6421 cgatgtatgg gctcggcatc gagatcgctc gcgagctcac ctcgacgact gcgcgcctca
     6481 cctcgctatt gggcgcgtgc gcgaccgacg cttcgaccgc gaacgatcgc gcttgtctcg
     6541 agacctttct gcggcgatgg gccgcgcgcg tcatgcgcac cgagctcagc agcgaagacg
     6601 tcgcattcta cgccgatatc gcgggaacca cgccggtcgc ggcggtcgcg gtggccgacg
     6661 tagtcgcggc ggtactcaac gcgcccgaaa ccttgtatcg cgtcgagcac ggtaccggta
     6721 cgtcgtcgcc aagcccgctg tcgagcttcg agctcgcggc gcggctctcg ttcaacctct
     6781 ggcaagagcc acccgacgac gagctctggg cggccgcttc caacggttcg ctgctgcaag
     6841 cgtcgacgta cgaaagccag ctggcccggc tagtctcgga tcccaaattg cgacgctcgc
     6901 tcgacgagtg ggtgagcgaa tggctgcgac tcggtgagct tccgcagctc gacacgctca
     6961 aggacgatcc cgcgttcgct gcctttgccg gctccgacct gccgacgcag tcgacgcgac
     7021 aggcgatgat cgacgatgtg ctcgagtccg cctggagcac gacgcgaagt gggcagcctg
     7081 cgagcgctct tctcgcggac cggcacgcct acgcaaaaga tccgattttg gcgcgcattt
     7141 acggcgtcac gcagtgggac ggcgtcggag aggctccgct cttcccgtcc ccgaaccgga
     7201 gcgggttgct cacgcgtgcg gcgctcttgg cgacgggcac ggccggcacc cgaccgatcc
     7261 acaaagggta cctcgtccgt aacgctctct tgtgccagca gctcggcgct ccgccactca
     7321 acgcgaacac ggaagtcccg gtcgcgaccg gacagctcac gacgcgggag gcggtttcca
     7381 accgaacttc tcccgcgagc tgcacgggtt gccattccat catcaacccc gcgggtttca
     7441 tcaccgagaa gttcgacgcg ctcggccgcg aacgcgacgc cgaaaggttg ttcgacgctc
     7501 gaggtcagct gatcgcagct ctcgcgctcg acaccgccgc ggctcccgga gtcattcccg
     7561 gcgacactcg cgtgatgcgc tccgcgccgg agctgaccga agccatcgac acgagcaggc
     7621 ttttccactc ctgcgtggcg cgtcactact tccgcttcgc ctccgcgaga gtggaagaca
     7681 cggagcgcga cggttgcttg ctcgccaaac tcgaagaagt ggcgcgcagc ggggcgtcgc
     7741 tcaaggcgct gctccaggcc ttcgctcaag accccacctt caagaacaag aggttcgagt
     7801 gagcaacggt tcccatctgc ttcgcatcaa tcgcaggttt ttcctacgcg gcgtgggggg
     7861 cacgctgctc gcgctgccga ccctgccatc tctactgcga ccgaagacgg cgcgggccga
     7921 ggccgccacc ccgcccaagt gcttcgtgca cttccggact ccgcacggcg gcatttcgac
     7981 cgccaacatg tggcccaacg aggcgacgct gaccgacacg ctcggctaca ctcacgacat
     8041 ccgccgcggc gcgttgaagg cgaccatcga tagcggcaat agcgtggtaa gtcccgttct
     8101 gaccgccagc tcgagcgtgc tcaccgcctc cctcgtcgcc aaaatgaacg tcctgcgcgg
     8161 cctcgatatc ccgatcgcga tggcgcacaa cttcggcgcg gcgctcggct actacgatgt
     8221 cgatcatcaa acgccggcgc gacccaccgc caccgtcgat caggtgctcg cgtattcgtc
     8281 cgcattctac ccggagctcg gctcggtaaa gcaacgcagc gtcgtcatct cggcgcccgg
     8341 cacgaatagc ggctcgcacg gctacgttac tcctggcgtg cgaacgtcgg gggtttccaa
     8401 agacgccctc tccg
//
LOCUS       DASTKS010000795         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_572286, whole genome shotgun sequence.
ACCESSION   DASTKS010000795 DASTKS010000000
VERSION     DASTKS010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572286"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFQ35_16485"
     CDS             <1..750
                     /locus_tag="VFQ35_16485"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9932304.1"
                     /translation="GEYELERGELLWFMPHSGHELVSASADFDMWVISFQPELVERVE
                     AKASGFDRVLSARRVPRTRLGSVALTWVEGALLSSFQLLCRGRAPEPPLMAQVLTRLL
                     RETVSEPPNAPAQGVRLSAEESRGDRLHSVTRRARAVLVTEPWLGRAEVAERLRVSES
                     TLAHSFRSDLGVTLDEFRNRVRFGRLLQLLDARPQSLLDAGLGAGFGSAAQYHRSVRA
                     LAGCRPGDLTSAATRRALSERVHATSRRSAD"
     gene            complement(730..3039)
                     /locus_tag="VFQ35_16490"
     CDS             complement(730..3039)
                     /locus_tag="VFQ35_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9932305.1"
                     /translation="MRTFAFVSLLLVAPWLAGCDSRGTTLGEQSGLPSGVDLLSNGQF
                     DQVSAPWMEYFAPGAAGTLGLESGQLCLTVTARGTNEWDAQMRARGISLQQGHRISVR
                     MLASSTPAARVSLKLAMQAAPYTAYWQAQVELGPEPRSVESSFVMSAASDDVAELVIM
                     AGASLTRSVPTKVCIDDVSVRDPEATLRPGENEPEPSVVRVNQLGFRTDASKLGVLVG
                     DGAEPRAFSLITDDGTEAFRAMSEPRGRDAASGDDVQWLDFSAFTAHCESCRLEVEGA
                     SSAPFRIADDVLAPLISSSLEYFYYNRSGTPIVMPYAGELRWSRPAGHLGDSHVTCLK
                     EHPCDYFLDASGGWYDAGDYGKYLVNGGLALFLLQNLFEHQRYSSLPAFDDGSLAIPE
                     GGNGVPDLLDEARWESEFFLRMQIPSGPLAGMAHHKLHGTDWTSLGTAPDEDTQTRYL
                     HPPSTAATLNLAATAAQASRLSREYDAPFAARSLAAAEAAYAAALAHPAEFAPNQSEG
                     GGPYDDVYVEDEFYWAASELWLTTNDSRYWDALRRSPLFGAVPSLVEPSRSDAGHYRA
                     LTWQNVEALGTFSLLLGLERLPADARESLRANLLSAAERYVSVSEHEGYRLPYAPGKD
                     GSYLWGSNVVVLSNAIVCAYAYDLVGDPRYLRVVQDALDYVLGRNALDRSYVTGVGER
                     PVRFPHHRFWSHSVRAERPPPPAGAMVSGANSALADTAVKSAVPSGAPPQRCHVDDIE
                     AYSVNEVAVHDNAALAWVATYLDSVRRAP"
BASE COUNT          560 a         1072 c         1051 g          567 t
ORIGIN      
        1 ggcgaatacg agctcgagcg cggcgagctc ctttggttca tgcctcattc gggtcacgag
       61 ctcgtgtcag cgagcgcgga tttcgacatg tgggtcatat cctttcaacc cgagctcgtg
      121 gagcgtgtcg aggcgaaggc cagtggcttc gaccgagtgc tttcggcgcg tcgcgtacct
      181 cgaacacggc tcggcagcgt cgcgctaact tgggtcgagg gcgcgttgct cagctcattt
      241 cagcttcttt gccgaggtcg cgcgcccgaa cctccgctca tggcgcaggt gttgacccgc
      301 cttttgcgcg agaccgtttc ggagcctccc aatgctcccg cgcaaggtgt ccggctttcc
      361 gcggaagagt cgcggggtga ccggctgcat tcggtgacgc gacgcgctcg cgcggtgttg
      421 gtgacggagc cgtggcttgg tcgggcggag gttgccgaac ggctcagggt gagtgagagc
      481 accctcgccc attccttccg cagcgacttg ggtgtgacgt tggacgagtt tcgcaaccgc
      541 gtacgcttcg ggcgcctttt gcagctgctc gacgcgcggc cacaatcgct tttggatgcc
      601 gggctcggcg ccgggttcgg tagcgcggcg caataccacc gctcggtgcg cgcgcttgcc
      661 ggctgtcgcc ccggtgatct cacgagcgcg gctacacgaa gagcgctttc cgagcgcgtg
      721 cacgcaacct caaggcgctc ggcggactga atcgagatac gtggctaccc aggcgagcgc
      781 cgcgttatcg tgaacggcga cctcgttgac gctgtacgcc tcgatgtcgt cgacgtggca
      841 acgttgcgga ggggccccgc tcggaaccgc gctcttcacg gcggtatccg cgagtgccga
      901 gttcgctccc gaaaccatcg ccccagcagg cggcggtggc ctctcggcgc gcacggaatg
      961 tgaccagaaa cggtggtggg ggaaacgcac ggggcgctca ccaaccccgg tgacgtacga
     1021 gcggtcgagc gcgttgcggc ccaaaacgta gtccaaggcg tcctgaacga cgcgtaaata
     1081 gcgcggatcg ccgaccaagt cgtatgcata cgcgcaaacg attgcgttcg agagcacgac
     1141 cacgttggag ccccacaagt agctcccgtc tttgccaggc gcgtacggca gccgataacc
     1201 ctcgtgctcg gagacgctca cgtagcgctc ggcagcgctg agaagattcg cgcgcaacga
     1261 ctcgcgagca tcggccggca acctttcgag cccgagcaac aggctgaagg tgccgagcgc
     1321 ctcgacgttt tgccaggtga gcgcgcggta gtggccagcg tcgctgcgcg aaggctcgac
     1381 gaggctcgga acagcgccga agagaggcga tcgacggagc gcgtcccaat atcgcgagtc
     1441 gttggtcgtc agccagagct cgctcgcggc ccagtagaac tcgtcttcta cgtagacgtc
     1501 gtcgtaaggt cccccaccct ccgattgatt gggagcaaac tccgctggat gggcgagcgc
     1561 cgccgcgtac gccgcctcgg ccgcagcgag gctccgcgcc gcaaacggcg catcgtactc
     1621 gcgggatagc cgagaagcct gagccgcggt cgcggccaag ttcaaagtcg cggccgtgct
     1681 cggcggatgc agataccgtg tctgggtgtc ctcgtcgggc gcggtgccca ggctcgtcca
     1741 gtccgtaccg tgaagcttgt gatgggccat ccccgcgagc ggcccactcg ggatttgcat
     1801 gcgcagaaag aattcgctct cccagcgcgc ctcgtccaat agatcgggaa cgccattgcc
     1861 gccttccggg atcgcgagcg acccatcgtc gaacgcgggg aggctcgagt atcgctggtg
     1921 ctcgaacagg ttctgcagca agaacagcgc gagcccaccg ttcaccaggt acttgccgta
     1981 gtctccggcg tcgtaccaac cgccgctcgc atccaagaag tagtcgcacg gatgctcttt
     2041 caaacaggtg acgtgggagt cgccgagatg gcctgcgggc cgcgaccatc gaagctcgcc
     2101 ggcgtaaggc atgacaatcg gcgtgccgct gcgattgtag tagaagtact cgagcgagct
     2161 cgagatcaaa ggagcgagca cgtcatccgc tatgcgaaac ggtgcgctcg aggcaccttc
     2221 gacctcgagc cgacacgact cgcagtgagc cgtgaacgcg gagaagtcca gccactgcac
     2281 gtcgtcgccc gaagcggcgt cgcggccgcg cggctcactc attgcgcgaa atgcctcggt
     2341 cccatcgtcc gtgatcagcg agaatgcgcg gggttctgcc ccatcgccga ccagcacccc
     2401 gagcttgctc gcgtcggtgc gaaaaccgag ttgattgact cgcacgacgc tcggctcggg
     2461 ttcgttttcg ccggggcgca gcgtcgcttc ggggtcccgc acgctgacgt catcgatgca
     2521 aaccttcgta ggcaccgagc gggtcaggct cgcgccggcc atgatgacca gctccgcgac
     2581 gtcgtcgctt gcggccgaca tgacgaacga gctttcgacg gaccggggct cgggtccgag
     2641 ctcgacttga gcttgccagt acgcagtgta cggcgccgcc tgcatcgcga gcttcagaga
     2701 gaccctcgcc gccggcgtcg agctcgccaa catacgaacg ctaatgcgat ggccctgttg
     2761 cagcgaaatg ccccgcgcgc gcatctgggc atcccattcg ttggtccctc ttgcggtcac
     2821 ggtgaggcac aactgacccg actcgagacc gagcgtcccc gccgcaccag gagcgaagta
     2881 ctccatccag ggcgcgctca cctggtcgaa ttgcccgttg gacaggaggt cgacgcccga
     2941 aggcaagccg ctctgttcac ccaatgtcgt tccgcggctg tcacagccgg cgagccacgg
     3001 cgcaacgagg agcaggctca cgaaagcgaa cgttcgcatc cgccgaggct acgctcggcg
     3061 aagctcacga tcgtctcatc tcgtgcaaaa accgttacgg ccctagggga tgtcccaagt
     3121 tccccgccct gctgcgagcc aagatcctgc ggctgcgaag cacggcctcg tcgctccgct
     3181 cctcgaaagg cttcagcctt cctccggtgc tcactcctcg ggcgcgcttc tcgctcgcgt
     3241 ttcttggctc
//
LOCUS       DASTKS010000796         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1018324, whole genome shotgun sequence.
ACCESSION   DASTKS010000796 DASTKS010000000
VERSION     DASTKS010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018324"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFQ35_16495"
     CDS             <1..381
                     /locus_tag="VFQ35_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932306.1"
                     /translation="PASEQKAASALLASSEPARALLRREAWLDGELSGQAPLELNGAF
                     ARKLAEIPLRNPQRSWSLFRRWLWAPALGWSIAAALGLFLGASFSDADEATSGATESA
                     QVAPDDPLDALALGDFTEWEETAP"
     gene            378..812
                     /locus_tag="VFQ35_16500"
     CDS             378..812
                     /locus_tag="VFQ35_16500"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET9932307.1"
                     /translation="MNPLTKRLAIVLAISLGINLLLGGFMLGRRFHGGPPPMASAHGR
                     DFPLLRERMRMKHGMGDMRPEWRAFQKDHRAARERVARAFEHEPFDPGELDGALAALR
                     EQTTRGQKALHDELAQQAKSGDEKLRQNLARSFRRPAMHGPE"
     gene            complement(878..1765)
                     /gene="ilvY"
                     /locus_tag="VFQ35_16505"
     CDS             complement(878..1765)
                     /gene="ilvY"
                     /locus_tag="VFQ35_16505"
                     /inference="COORDINATES: protein motif:HMM:NF008722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HET9932308.1"
                     /translation="MDSDALRLFLHLARTLHFTRTSRECNISLSALSRTVQRLEAEVG
                     QPLFLRDRRSVSLTSAGELFREYAADALSRLQVLRQRMEGTAEQLSGTIRIFASVTAV
                     QSFLPRILTTFRRTHPEIHIELETGYALDALARLEQGAVDVSVAALPARVPPNLEARV
                     VVVTPLVFVAPASECEASRLVERRPVPWGEVPMVLPASGLARAAVDRWFARRKLHARL
                     YGEVAGNEAILSLVSLGCGVGVVPRLVADKSPLRGELQVLEVEPRLGEFRVGVCTEKR
                     SLKTPLVRAFWDSIDEARA"
     gene            complement(1814..2998)
                     /locus_tag="VFQ35_16510"
     CDS             complement(1814..2998)
                     /locus_tag="VFQ35_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932309.1"
                     /translation="MRSATCSVLFLVVGFGSVEAQAESSLESSLCGERRGCRIDKSLA
                     SLKMRDGGRHELVRLTLPEEGEPIENDANCTFEEFWLVARDAQGGVLESHLFAGGCSR
                     DSPQEAWCGAPPRASVRVRGTVVEARWDAHALRCMSVYHSAGSYEASLESLLPLREES
                     RYFRVVPPTEEIRRSWDFTTMRGSWGAYAGADTCAALRRGPIPRIPAPTIAPEFLEAA
                     WQGADMSRCATTIDSVYGFAPTGHAAKVKLLLLGSREENLFVEVVPTRAQRKLPSDAI
                     LRVCYTDYAVDSYAYCHTQPKIECTRFGMDGRVLDGELRVERSSERARFKVHLPAEAQ
                     SFTVSYTEPSSGASLASSQQRPLNFTYMNDLFPIAEEVGSCELGDDGLRMRLGPQSRA
                     PE"
     gene            3191..4564
                     /locus_tag="VFQ35_16515"
     CDS             3191..4564
                     /locus_tag="VFQ35_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932310.1"
                     /translation="MSLRSLSYISFGLTIAALGCSASSDAGTAEGETSGNVELAIERV
                     PDDAACLRVAASAGRTVSKTFKLTAGASQTFSLDRLPLGVARVDAEVYPLSCEKVQAS
                     SVPNYVTDSSTLVRIDPLVPAQVVLKLIRNGRANVKVDFETPPWIDASRGPVELALIG
                     DTPYGAAQIADFPAFIASIGNAPNLREVVHLGDIKNGSSRCDDSYFQFVLDNFESSAI
                     PFVYTPGDNEWTDCHRANNGAYDPLERLATLRSMFFPTPGLSLGRAPRQVESQSFYPG
                     FETFVENQLWVEAGAVFAAVHVVGSNNSLLAWYTDDRTGTKLDDPARRQAEVEARTRA
                     NLAWLDHAFSVATEQSAVGVVLLMQADMWDGSPTNGFDSTVQKIASQSLAFGKPVLLV
                     EGDSHVFKADNPLAAGDPTHGVTTPVPNLTRIVVQGSTTAPLSEWLRLRVDPSQAQPF
                     SFTRNPR"
BASE COUNT          878 a         1655 c         1470 g          788 t
ORIGIN      
        1 ccggcgagtg agcagaaagc cgcctcggcg ctgctcgcga gctccgaacc agcgcgcgcg
       61 ctgttgcgtc gcgaagcttg gctggatggg gagctttcgg ggcaagcgcc gctcgagctg
      121 aacggcgctt tcgcacgaaa gctcgccgaa attccgctca gaaatccgca gcgctcctgg
      181 tccctgttcc ggcggtggtt gtgggcgccc gccctcggct ggagcatcgc agcggcgctc
      241 gggttgttcc tgggcgcgag cttctcggac gcggacgaag cgacgagcgg agccaccgag
      301 agcgctcaag ttgcaccaga cgaccccctc gacgcgctcg cgctcggcga ttttaccgag
      361 tgggaagaga ccgcaccatg aacccgctga ccaagcgcct cgcgatcgtg ctcgccatct
      421 cgctcggcat caacctgctg ctcggtggtt tcatgctggg ccggcgcttc cacggaggtc
      481 ccccgccgat ggcttccgct cacggccgtg acttcccgtt gttgcgcgag cgtatgcgaa
      541 tgaagcacgg catgggagac atgcggcccg agtggcgcgc cttccaaaag gatcaccgcg
      601 ccgcgcgcga acgcgtcgcg cgcgctttcg agcacgagcc gttcgacccg ggcgagctcg
      661 acggcgccct cgctgcctta cgcgaacaga cgacccgcgg acagaaggcg ctccacgacg
      721 agctcgctca gcaagcgaag agcggcgacg aaaaactgcg acaaaacctc gcgcggtctt
      781 tccgcaggcc ggcgatgcac ggtccagaat gaaccaagag cccgcgtcgc actaagagcc
      841 aatcctaaat ccctcccgtc caggattagg actgaatcta agcgcgggct tcgtcgatcg
      901 agtcccagaa cgcgcggacc agcggggtct tcagcgagcg cttttcggtg cacacgccca
      961 ctcggaattc tcccaagcgc ggctcgacct ccagcacttg cagctcgcct cgtagcgggc
     1021 tcttgtccgc gacgaggcgc ggaacgacac cgaccccaca cccgaggctg acgagcgata
     1081 agatcgcttc gtttccggcg acttcaccgt agagccgcgc gtgcaactta cggcgagcga
     1141 accaacggtc gacggccgcg cgcgcgagcc ccgacgccgg gaggaccatc ggcacttctc
     1201 cccagggaac cgggcgacgc tcgacgagcc gactcgcttc gcactccgag gcgggcgcca
     1261 cgaagacgag cggggtcacc accacgacgc gcgcctcgag gttaggtggt actcgcgccg
     1321 gcagcgcggc cacgctgaca tccaccgcgc cctgctcgag ccgtgcgagg gcgtcgaggg
     1381 cgtatccggt ctcgagctcg atgtgaattt cgggatgagt gcgccgaaat gtggtgagga
     1441 tgcgcggcaa aaagctctgc acggccgtca ccgacgcgaa gatgcgaatg gtcccgctca
     1501 actgctcggc ggtcccttcc atacgctgcc gcaagacttg caggcgggag agcgcgtcgg
     1561 cagcgtattc acgaaatagc tctccggcgg acgtgagcga cacgctccga cggtcgcgca
     1621 aaaacagagg ctgaccgacc tctgcctcga gccgctgcac cgtccgtgaa agcgcggaca
     1681 ggctgatgtt gcactcgcgg ctcgtccgcg tgaagtgcag cgtgcgcgcg agatgcaaaa
     1741 acaaccggag cgcgtcagag tccatgttgc gatatcagca acacggtatg ccacgccttt
     1801 cgcgccgagc aagctactcg ggagccctcg attgcggccc caagcgcatg cggaggccgt
     1861 cgtcgccgag ctcacacgac ccgacctcct cggcgatggg gaacagatcg ttcatgtaag
     1921 tgaaattcag cgggcgctgc tgactcgaag cgagcgaagc tccgctcgag ggctccgtat
     1981 acgaaacggt gaacgactgc gcttccgcgg gcaggtgaac cttgaagcgc gcgcgctcag
     2041 acgagcgttc gacccgaagc tcgccgtcga gcacccgccc atccatcccg aaccgagtgc
     2101 actcgatctt cggctgcgtg tggcagtagg cgtacgaatc gaccgcgtag tccgtatagc
     2161 agacacgcag gattgcgtcg ctcggcaact ttctctgcgc tcgggtcggc acgacctcca
     2221 cgaacaggtt ctcctcgcgg ctgcctaaca gcaagagctt caccttggcg gcgtgccccg
     2281 tcggcgcaaa gccgtaaacc gaatcgatcg tcgtcgcgca acgactcatg tcagcgcctt
     2341 gccaagccgc ttcgaggaat tctggcgcga tggtcggcgc agggattctc ggaatcggcc
     2401 cgcgacgtag agcggcacac gtgtcggccc ctgcatacgc tccccaggag ccgcgcatcg
     2461 tggtgaagtc ccaagaccgt cggatctctt cggttggcgg gactacgcga aagtagcgcg
     2521 actcctcccg cagcggcagc aagctttcga gcgaggcttc gtagctgccg gctgagtgat
     2581 acacgctcat gcagcgcagt gcgtgggcgt cccagcgcgc ttcgaccacg gttccgcgga
     2641 cgcgcacgct cgcgcgcgga ggagcgccac accaggcttc ctgcgggctg tcgcgactgc
     2701 agccaccagc aaacagatgg gactcgagca cgccaccctg cgcgtcgcgc gccacgagcc
     2761 agaactcctc gaacgtgcag ttcgcgtcgt tctcgatggg ctctccctcc tccggcaacg
     2821 tcaagcgaac gagttcgtga cggccgccat ctcgcatctt gagcgaagcc aacgacttgt
     2881 cgatccgaca gccgcgccgc tcgccgcaga ggctcgactc gagcgacgac tcggcctgtg
     2941 cctcgacgct accgaacccc accaccagga agaggacgct acacgttgcc gatcgcatgg
     3001 gccgcgcgct cagcaaccct cgtaccagct cgcgtttggg cggaatcgac ggggagacgc
     3061 ggccaggcgc ccacaccgcg tgtctcgccg tccacgagcg agttcgcaac gacaacgtca
     3121 cgccgaattc ctggcgacgt gaccgcgcct ccgcacgcgc tcggtatcag gcgcgcttct
     3181 cggaggttcc atgtcacttc gctcgctctc gtatatctcc tttggattaa caatcgccgc
     3241 gctgggctgc agcgcgagct ccgacgccgg cactgctgaa ggcgaaacca gcggcaacgt
     3301 cgaactcgcc atcgagcgcg tccccgacga cgcggcgtgc cttcgcgttg ccgcatcggc
     3361 tggccgcacc gtgagcaaga ccttcaagct cacggcgggc gcgtcgcaga ccttctcgtt
     3421 ggatagactg ccgctcggcg tcgcgcgcgt ggacgccgaa gtctatcccc tctcttgcga
     3481 gaaggttcag gcgagcagcg ttcccaacta cgtcacggac agctccacgc tcgtccgcat
     3541 cgatcccttg gttccggcgc aggtcgtctt gaagctgatc cgcaatggac gcgcgaacgt
     3601 caaagtggac ttcgagacgc cgccctggat cgatgcgagc cgcggtcccg tcgaactcgc
     3661 gctaataggc gacacgccgt acggtgcggc ccaaatcgcc gactttccgg cgttcatcgc
     3721 ttcgattggg aacgctccga accttcgcga agtcgtacac ctcggcgaca taaagaacgg
     3781 cagcagccgc tgcgacgact cctacttcca gttcgttctc gataacttcg aaagctctgc
     3841 tatcccattc gtctacacgc ccggcgacaa cgaatggacc gactgtcatc gtgcgaacaa
     3901 cggcgcgtac gatccgctcg agcgcctcgc aaccctccgc tcgatgttct ttccgactcc
     3961 gggcctttcg ctcggaaggg ccccgcgcca agtcgagagc cagtcgtttt atccaggctt
     4021 cgagaccttc gtcgagaacc aactctgggt cgaggcgggc gcggttttcg cagccgtaca
     4081 cgtcgtcggc agcaacaaca gcctgctcgc ttggtacacc gacgatagga ccgggaccaa
     4141 gctggatgac cccgcgcgcc gccaggccga ggtggaggcg cgcactcggg cgaacctggc
     4201 ttggctcgac cacgctttct cggtcgcgac agagcagagc gcggttggcg tcgtactcct
     4261 gatgcaggct gacatgtggg acggttcgcc caccaacggc ttcgactcga cagtgcagaa
     4321 aatcgcctcg cagtcgctcg ctttcggaaa gccggtgctg ctcgtcgagg gagactcgca
     4381 cgtcttcaaa gccgacaatc cgttggcggc cggcgacccc acgcatggcg tgacgacccc
     4441 ggtgccgaac ctcacccgca tcgtcgtgca aggctcgacg acggcgccgc tctccgagtg
     4501 gctacgcctc cgcgtcgatc cgagccaagc tcagcccttc agcttcacgc gcaacccgcg
     4561 ctgaacgctc gcaacgagtt cgagcagcgc cgtaatgagc tccggcgctg ctcgccgaag
     4621 tgagacgttc gtaacgaggc attgcttcat ccgtctcgct ttcttcggtc tactcagtga
     4681 ggccttccaa cctcatcatt gcgggctgat cgcggcttct acccgcagcg gacgccagct
     4741 gtgccaccgg cggccaaccg tctggctcaa acatcgcgac atccgcacca g
//
LOCUS       DASTKS010000797         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1018353, whole genome shotgun sequence.
ACCESSION   DASTKS010000797 DASTKS010000000
VERSION     DASTKS010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018353"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VFQ35_16520"
     CDS             complement(<1..847)
                     /locus_tag="VFQ35_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9932311.1"
                     /translation="MKPDAAPGTWIESDVPARLDALGWSGWHRRVVIALGITWILDGL
                     EASLIANLGPSLRDPRTLGLSAAQIGLSNSAYLAGQVLGALVFGHLTDRWGRKRLFLV
                     TLCLYLVATALSGLAPTFAIFAAFRALAGAGIGGEYSAVNSAIDELIPARLRGQIDLG
                     INGSYWIGVALGAGMTLVLLNPSWVPLSLGWRLVFGTGSILGLAILLVRRHVPESPRW
                     LLMHGHVERARQTLAAIELEVLGRDSHPKLRPVRLQVTGTVGLRYLLRTLLVRHPRRT
                     VLGLSL"
     gene            complement(947..1657)
                     /locus_tag="VFQ35_16525"
     CDS             complement(947..1657)
                     /locus_tag="VFQ35_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932312.1"
                     /translation="MRIGSKLGSVLVVVGLGCVTRAANAADEFGAPKTFSVSAERLSG
                     VVFASETHDTAGTTVTSKRTTISFMGSALAGAVNVYSLPRIAFDVFPIKGLSLGGSLA
                     LINISANSETEAGGISSSTDGTNIFGLLFSPRVGYAVMFNPKVGIWPRGGFTYWWTKA
                     TPNDSSVDASQSTSRWAVTLEAPFVISPAPHAAIYVGPTLDLGVAGSNTSKQGSTETS
                     VDYKGTDIGLQAGLGLYF"
     gene            complement(1849..>3399)
                     /locus_tag="VFQ35_16530"
     CDS             complement(1849..>3399)
                     /locus_tag="VFQ35_16530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9932313.1"
                     /translation="LMGVERFGSNRADLVDDLELEQRGSETRLRAVRAIGESGETSEA
                     PPRVLLALLAGHERELRRLCLRLDVPRDGFVSIWLERLSHSALHADEASIVAPALRSM
                     GNPLLESLGSGRLERAELAADELFGVLRASSLGVNSALALIGAFEDAAARRAAGAELS
                     PTIRAVGQFFTCVAVRAFGGGAHSPALEQSSERVRAKVERRSAATYLVGESRCMVELR
                     AAIADLAMTRGAVLIVGESGTGKELVAKALHELGPLAQEPFVAINCAALPRELIESEL
                     FGHERGAFTGSRESAPGLLRAAGGGSVFLDEITEMPQALQPKLLRALEQRAVRPVGGL
                     RELPFRARIVAATNADPEALGESQRLRPDLFYRLCVHRIDVPPLRERCGDIPLLVEHF
                     LRELECGGLVPEFSAEAIAALQRYPWPGNVRELRNAVEHASATAKGGRIEVRHLPARV
                     TRRDESTSLNVSRSMPMGDELSLRLVERRHIEHVLQLSNGNKARAARLLGLSRHQLYL
                     KLERFGIG"
BASE COUNT          657 a         1159 c         1070 g          513 t
ORIGIN      
        1 tcaaagagag accgagcacc gtccgacgcg gatggcgaac gagcagcgtg cgcagcagat
       61 agcgaaggcc gaccgtgccg gtcacctgca accgcaccgg cctgagcttt gggtgggagt
      121 cacggccgag cacctcgagc tcgatcgcgg ccaaggtttg gcgcgcccgc tcgacgtgac
      181 cgtgcattaa gagccagcgc ggactctccg gtacgtgccg ccgcacgagc agaatcgcga
      241 gccccaagat cgagccggtg ccgaacacga gccgccatcc gagagagaga ggcacccagc
      301 tcgggttcaa caacacgagc gtcatgcccg cgcccaacgc gactccgatc cagtagctgc
      361 cgttgatacc gagatcgatc tgccctcgaa ggcgagccgg gatgagctcg tcgatggcgg
      421 agttgaccgc cgagtactca ccgcctatcc cggcccccgc caacgcgcga aatgccgcaa
      481 atatcgcgaa cgtcggggcg agcccgctga gcgccgtcgc caccagatag agacacagcg
      541 tgaccaagaa caggcgcttc cgtccccagc gatccgtcag gtgaccgaac accagcgcgc
      601 cgagcacttg gccggcgagg taagccgaat tggagagccc aatctgggcc gcgctcaagc
      661 ccaacgtccg tgggtcccgg agcgaggggc cgaggttcgc gatgaggctt gcctcgagcc
      721 cgtcgagaat ccaggtgata ccgagcgcga tcacgacgcg acggtgccaa ccagaccaac
      781 ccagcgcgtc gagccgcgcc ggaacgtccg attcgatcca ggtgccgggt gctgcgtcgg
      841 gcttcacgac ggatacgcgc catcacgcta acacgtctcg tcgaaagccc ttcccgtcaa
      901 atacgccaca gagcggcgcc ttcgcagcac gaccaacggg gaactctcag aaatagaggc
      961 ccagcccagc ctgcaatccg atgtcggtgc ctttgtagtc gacgctggtt tcggtcgagc
     1021 cttgcttgga ggtgttgcta ccggcaacgc cgaggtcgag cgttggaccc acgtagatcg
     1081 cggcgtgagg ggcgggagaa atcacgaaag gagcctccaa cgtgaccgcc cagcggctcg
     1141 tggattggct cgcgtcgacg gagctgtcgt tcggcgtggc tttggtccac caataggtga
     1201 aaccaccgcg aggccaaata ccaaccttgg ggttgaacat cacggcgtaa ccaacccgcg
     1261 gcgagaacaa caaaccgaaa atgttggtgc cgtccgtcga cgacgagatc ccccccgctt
     1321 cggtctcgct attggcggag atgttgatga gcgcgaggct tccgcccagg ctcaagcctt
     1381 tgatcggaaa cacgtcgaac gcaatgcgag gcagcgaata gacattcacc gcccccgcga
     1441 gagcgctgcc catgaaagat atggtcgtgc gcttcgaggt gaccgtagtg ccggccgtat
     1501 cgtgcgtttc cgacgcaaaa accactccgc tcagcctctc cgcactgacg ctgaacgttt
     1561 tgggtgcgcc gaactcgtcg gcggcgttcg cggcccgcgt cacgcacccc aaaccaacca
     1621 ccaccaacac cgaacccaac ttgctcccga ttcgcatcct tcctcctgtc ttcgagcgag
     1681 ggagcgtcgc actacgccgc gcggaaggtc aagggcgctc actcgcaccc acacgaggaa
     1741 caatcgccga gcgctgtgat cttcgcgctg acgcgcgtca cagtgtcaac cgatgccgaa
     1801 gcgttcgagc ttcaggctgt gatcttcgcg ctgacgcgcg tcacagtgtc aaccgatgcc
     1861 gaagcgttcg agcttcaggt agagttggtg gcgcgataac cccagcaatc gcgcggcgcg
     1921 cgccttgttt ccgttcgaga gctgcagaac gtgctcgata tgacgccgct cgacgaggcg
     1981 cagcgagagt tcgtcgccca tgggcatcga acgcgacacg ttcagagagg tcgactcgtc
     2041 acgtcgcgtg acgcgcgcgg ggaggtgccg cacttcgatg cggccgccct tcgccgtcgc
     2101 ggacgcgtgc tcgaccgcgt tccgcagctc gcgcacattt cccggccacg ggtagcgttg
     2161 aagcgccgcg atggcttctg cgctgaactc gggcactagc ccaccgcact cgagctcgcg
     2221 cagaaagtgc tcgacgagca gcgggatgtc tccgcagcgc tcgcgcagcg gcggaacgtc
     2281 gattcggtga acgcacaaac gataaaagag gtccggcctc aagcgctgcg attcaccgag
     2341 cgcttccggg tcggcgttgg tcgcagccac gatgcgcgcc cgaaacggca actcgcggag
     2401 tccgccgact ggtcgcaccg cgcgctgctc gagggcgcgc aggagcttcg gttgcaatgc
     2461 ctggggcatc tcggtgatct cgtccaggaa cacgctgccc ccaccagccg cgcgcagtag
     2521 ccccggcgcg ctctcgcggc tgcccgtgaa cgcgccacgc tcgtgtccga agagctcgct
     2581 ctcgatgagc tcccgcggga gggccgcgca gttgatggca acgaacggct cctgcgcgag
     2641 tggaccgagc tcgtgcaacg ccttggcgac cagctcctta cccgtgccgc tctcacctac
     2701 gatcagcact gcacctcgcg tcatggcgag atccgcaatg gcggctcgga gctccaccat
     2761 gcaacgactc tcaccgacga ggtaagtcgc cgccgagcgc cgctctacct tggcgcggac
     2821 gcgctcgctg ctctgctcga gggcgggcga gtgcgcgccg ccgccgaatg ctcgcaccgc
     2881 gacacacgtg aagaattgac ccacggcgcg gatcgtcggc gagagctcgg ccccagcggc
     2941 gcgccgggcg gccgcgtctt cgaacgcgcc gatcagcgcg agcgcggagt tgacccccaa
     3001 agaagaagcg cgcaaaacgc cgaagagctc gtcggccgcg agctcggcgc gttccagtcg
     3061 accgctgccc agggattcga gcaggggatt ccccatactg cgcaacgcgg gagctacgat
     3121 cgaagcctcg tcggcgtgca aggcggagtg cgagagccgc tccagccaaa tcgagacgaa
     3181 gccatcgcga ggaacgtcga gcctcaggca caaccggcgc agctcgcgct cgtgtccggc
     3241 gagaagcgcc agcaagacac gcggcggtgc ttcactcgtc tcaccacttt cgccgatcgc
     3301 gcgcaccgcg cggagtcgag tctcactccc ccgctgttcc aattcgagat cgtcgacgag
     3361 atccgcgcgg ttcgagccga agcgttctac tcccatgag
//
LOCUS       DASTKS010000798         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1018502, whole genome shotgun sequence.
ACCESSION   DASTKS010000798 DASTKS010000000
VERSION     DASTKS010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018502"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFQ35_16535"
     CDS             <1..881
                     /locus_tag="VFQ35_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HET9932314.1"
                     /translation="GESRTLNVEFAPASGQSETSTLNIRNTSSNLASPSLSLAGTGTS
                     NANSAPIQVFITQQNAVNDNSVSLRAEVKNRGTSAIRLSDYRVVYYLSDPEQDPTTWV
                     WDTAYTTVGPITAKAKKLLATKKVGARKADTALTFTFANTTVAAGASAIFQGTLHRAD
                     YSWYPDETDDWSRYLRRDGMAEGTTVQLISNNSTVFGVGGDLQPGAHQFKLSPTTVSS
                     SVTSTFVMDASDGYERTYRFFTPSGTLVKSIWTYMSAPGQQTETLELSDLPAGTYTVI
                     LELGGNAADLTTITKL"
     gene            complement(962..1438)
                     /locus_tag="VFQ35_16540"
     CDS             complement(962..1438)
                     /locus_tag="VFQ35_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932315.1"
                     /translation="MRRIYGLLAPLFASCLLSVHAEADNTPANCRSVSGTFTVITSTG
                     GDCKSPVGLCGSVEWRGDLRAKSSFVATSSVETVDSAVTSVLVFTGDATVTAKGGTIL
                     TKDAIVFRLTGAGDFAEVDTVVGGTGVFSNASGAWRASGTFAGNVGEGRYEGMICR"
     gene            complement(1650..2630)
                     /locus_tag="VFQ35_16545"
     CDS             complement(1650..2630)
                     /locus_tag="VFQ35_16545"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HET9932316.1"
                     /translation="MGHDSRLSALSRRRFLKSVAAAGGALGCAEIPPVTRPDPARFDA
                     CPNSRALSILTWNIFMMPEWIKESPFNQSRARAIAATLLEQQFDILCLQKVFDRAALA
                     ILENALGRRYPHRYGPANDGCLLLSSGVWVLSRYPLVDYQQIEFSECSSWECFARKGA
                     LLLSGTCGKTPFRLVTTHLQGEEGSSFTRANQAVRDRQMRQIRDRLIVPHLEAEVPFI
                     ICGDFGTPRFAKSHGDETDAYRSMLTTLGVENGATWRITLDEIDSQLAKSASARRNEL
                     EYVFVRKNGFPLEVERMRHVFERDGWDPKQHHTDLSYRYAVSAKLSFGQA"
     gene            complement(2609..>3042)
                     /locus_tag="VFQ35_16550"
     CDS             complement(2609..>3042)
                     /locus_tag="VFQ35_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9932317.1"
                     /translation="YKAIWDALEAAGIPFTCHWGQLNGMTPERLSTYFGPRVQAWKSA
                     RTKLLGENSPALEVFAAPILKQVGLDGVASTQPPPVQPHPVIFRGSRGPAVALWQAAI
                     GVGADGIFGPATETATRSWQAAHNLSADGVVGPASWATIRA"
BASE COUNT          598 a          998 c          935 g          511 t
ORIGIN      
        1 ccggcgagtc gaggacgctc aacgtcgagt ttgcgcccgc ctctggccag agcgagacct
       61 cgacgttgaa cattcgcaac acgagctcga acctggcgag ccccagcctt tcgctagctg
      121 gaacaggcac gagcaacgcc aacagcgctc cgattcaggt tttcatcacg cagcaaaacg
      181 cggtcaacga caactcggtg tccttgcgcg ccgaagtgaa gaaccgcggc acgagcgcga
      241 tccggctgtc tgactatcgc gtcgtctact acctgagcga tcccgagcag gatccgacca
      301 cttgggtgtg ggacaccgcg tacacgacgg ttggacccat cacggccaag gccaagaagc
      361 tgctcgcgac caagaaggtc ggcgctcgga aggccgacac cgcgctcacg ttcacgttcg
      421 cgaacacgac cgtcgccgct ggcgcctcgg ccatctttca aggcaccctg caccgcgcgg
      481 actacagctg gtatcccgac gaaaccgacg attggtcgcg ctacctgcgc cgtgacggaa
      541 tggcggaggg cactaccgtt cagctgattt ccaacaacag cacggtcttc ggcgtcggcg
      601 gtgatctcca gccgggcgct caccagttca agctcagccc gacgacggtt tctagctcgg
      661 tgacctccac gttcgtcatg gacgcgagcg acggttacga acgaacctat cgcttcttca
      721 ccccgagcgg caccctcgtg aagtcgatct ggacgtacat gagcgctccg ggtcagcaaa
      781 ccgagacgct cgagctctcc gacctgcctg ccggcacgta cacggtgatc ctcgagctcg
      841 gcggcaatgc cgcagatctc accaccatca ccaagctctg agtttcgcac tcgaggtaaa
      901 agggagggcg ctcgcaccgc ggggcgccct cttcgttact cgaggcggaa agagaatgga
      961 ttcaacggca gatcatgcct tcgtagcgtc cctcgccgac gttgccggcg aaagtgccag
     1021 atgcgcgcca cgcgccgctc gcattcgaaa agacaccggt cccaccaacg acggtgtcga
     1081 cctccgcaaa gtctcctgcg ccggtcaggc gaaacacgat ggcgtccttg gtgagaatgg
     1141 tgccaccctt ggccgtcacg gtggcgtcgc cggtaaagac cagtacggat gtcaccgcgc
     1201 tgtccaccgt ttcgacactg ctcgtcgcga cgaagctcga cttggcgcgc aaatcgccgc
     1261 gccactcgac cgaaccgcag agcccgacag gagacttgca gtcgcccccg gtcgaggtga
     1321 tgacggtaaa ggtgcccgaa accgagcgac aattcgccgg cgtgttgtcg gcttcagcgt
     1381 gcacagacag caagcaagag gcgaacaagg gagcgagtag accgtaaatt cgacgcattg
     1441 ggtaacctcc acgcgccagt acgaagcgtt tcgagctcgg ttacctcgag agccaacgtc
     1501 aacctcacgc cggcgagccg tgaccgaacc gtcagctcgt tgcacccgac gatggctctg
     1561 ccttcacgag acgggttggg ccaagggccc gtgccgatgg cgaggctcga ggcgggccgc
     1621 gctcgagcga acccgcgcgc ccgaagcgac tacgcctgtc cgaaagagag cttggcgctg
     1681 accgcgtagc gatacgacag gtcggtgtgg tgctgtttgg gatcccagcc atcgcgctcg
     1741 aagacgtggc gcatgcgctc gacctcgaga gggaacccgt tcttcctcac gaacacgtac
     1801 tcgagttcgt tccggcgggc gcttgccgac ttggcgagct gactgtcaat ctcgtccagt
     1861 gtgatgcgcc aggttgcgcc gttctcgacg ccgagcgtag tgagcatgct gcgataagcg
     1921 tcagtctcgt ccccgtggct cttcgcaaag cgaggtgtgc cgaaatcgcc gcaaatgatg
     1981 aacggcactt ctgcctcgag atgcggcacg atcagccgat cccggatctg ccgcatttgt
     2041 cgatcgcgaa ccgcctggtt cgctcgcgtg aacgaagacc cttcttcacc ttgcaagtgg
     2101 gtggtgacca ggcgaaacgg agtcttcccg cacgtgccgg agagcagtag cgcccccttg
     2161 cgcgcgaagc actcccaaga gctgcattcg ctgaattcaa tctgctggta gtcgacgagc
     2221 gggtaacggc tgagcaccca gacgccgctg ctcagcaaca aacatccgtc gttcgcggga
     2281 ccatatcgat gcggatagcg acggcccagg gcattctcga ggatcgcgag cgcggctcgg
     2341 tcgaagacct tttgcagaca caggatatcg aattgctgct cgagcaacgt tgccgcaatg
     2401 gcgcgagctc gcgactgatt gaacgggctc tctttgatcc actccggcat catgaagatg
     2461 ttccaggtca agatcgagag agcgcgtgag ttcgggcagg cgtcgaagcg cgcgggatcg
     2521 gggcgcgtga cgggcggaat ctccgcgcac ccgagcgcac cccccgcggc ggccacgctc
     2581 ttcagaaacc gacggcgcga gagcgcgctc aggcgcgaat cgtggcccat gatgctggtc
     2641 cgacgacccc atccgccgac aggttgtgcg ccgcttgcca gctgcgcgtc gccgtctccg
     2701 tcgcaggacc aaatatgccg tcggcgccca cgccaatcgc ggcttgccag agcgcgaccg
     2761 ccggaccgcg cgagccgcga aaaatcaccg ggtgtggctg aaccggcggc ggctgtgtag
     2821 acgccacgcc gtcgagcccg acctgcttca gaatcggcgc tgcaaaaact tcgagcgccg
     2881 gactgttctc gccgagcaac ttcgtgcgcg cggatttcca tgcctgcacg cgcggcccga
     2941 agtacgtgct caagcgctct ggggtcatgc cgttgagctg gccccagtga cacgtgaacg
     3001 gtatgccggc ggcctcgagg gcgtcccaaa tcgccttgta ga
//
LOCUS       DASTKS010000799         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1242846, whole genome shotgun sequence.
ACCESSION   DASTKS010000799 DASTKS010000000
VERSION     DASTKS010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242846"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1856
                     /locus_tag="VFQ35_16555"
     CDS             213..1856
                     /locus_tag="VFQ35_16555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9932318.1"
                     /translation="MPWVALVGPEIEENLSLRYIASSLGVAGYSSEIVPFNNEGDFGS
                     ALEAILSAAEPPVVVGISLAFQWRAQDFLALVVALRQGGYRGHVTVGGHFATFASLEL
                     MSDFPELDSVVRQEAEETMVALTRAVAQGQALDGIPGLALRSTDGRAWLTPHPALPDI
                     AKLPRPDRRGEPASCFAHPIAPLVSSRGCYANCSFCCIAAWQEQSLPGKRYRLRDIED
                     VADEMVEMQRARGIDIFVFHDDNFFVPGHKKNAARFSALADALERRQIGPFATVVKAR
                     PTDVDPLVFEILRRRLNCIRVYIGIETDADQGLETLRRWSQAKQNKRAIEVARALELY
                     TCFNLLVFDPDTTLESLRTNIAFMRFACEYPSNFGRVELYAGTPLLARMQAEGRTSGD
                     YMQWDYRLASPELERVFELAMDCFHERNFGPEALNNRIMGTRFDVEVCRHFHPDRFDP
                     AWLAEGKSLTSELSLDSARALEAIVSHVEQRAPNEDRALVLALSKQLRATERAVGEAA
                     RELARKLQRAVGRGSPLTDIGDRVATPLQTQRLAAGAMP"
     gene            1853..2272
                     /locus_tag="VFQ35_16560"
     CDS             1853..2272
                     /locus_tag="VFQ35_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932319.1"
                     /translation="MSNRRRLKLMNEVLPSPTNAGASSPRARTLLHMQRLLSATAFAV
                     ACSKQTAPEPPGYGVVDPMPTPARDRDEPDASAPIPVPISDPKVDGGTAQFAEPPPSA
                     SQSAKPPKGKPPKQPEPPGYGVVDPMPPPAKRNRSEP"
     gene            complement(2334..2924)
                     /locus_tag="VFQ35_16565"
     CDS             complement(2334..2924)
                     /locus_tag="VFQ35_16565"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9932320.1"
                     /translation="MNGAGMSKGRLEAFSDGVFAILITIMVLELKIPHGTDWGAIRHL
                     VPVFLTYVMSYVFLGIYWSNHHHMLHATEHISGGVLWANLHLLFWLSLVPFVTGWMGE
                     NHFAALPTAAYGLVLLMSAVSYTLLQNVILSVQGPHSKLSVAVGRDRKGKISLVFYAL
                     AILLAFVNQFISDALYALVALIWLVPDKRIEHTLSR"
BASE COUNT          560 a          973 c          928 g          485 t
ORIGIN      
        1 gtggggtgga cggcgttgcc gacaccggcg cggtgctgcg cgtccggttg tgaggggacg
       61 ggagccagtg acggcggcaa cgaacccaaa gatgggctgc gccactacca cacggcgatg
      121 aattgcccaa gcggcggccg cgtgccgctg aacccctgaa ccgtcttgcg tctcgatgcg
      181 aagcggaaga ccgttacgct cggaggtgcg agatgccgtg ggtggcgctg gtcgggcccg
      241 agatcgaaga gaacctcagc ttgcgataca tcgcttcgtc gctgggagtc gccggctact
      301 cctccgagat agtcccgttc aacaacgaag gcgactttgg ctcggcgctc gaagcgatcc
      361 tgagcgccgc tgagcctccc gtcgtcgttg gcatctcact cgcattccag tggcgagcgc
      421 aagacttctt ggcgctcgtg gtcgcgctgc ggcagggcgg ctatcgcggt cacgtaacgg
      481 tgggcgggca tttcgcaacg tttgcgagcc tcgagttgat gagcgacttt cccgagctcg
      541 attcggtggt gcgccaggag gcagaagaga cgatggtggc gctcacacgc gccgtggctc
      601 agggacaggc gctcgatggg atccccggtc ttgcgctgcg gagcacggac ggacgcgcct
      661 ggctgacgcc gcacccggcc ctacccgaca tcgccaaact gccgagaccg gatcggcgcg
      721 gcgaacccgc gtcgtgcttc gcgcacccca tcgctcccct cgtgtcgagc cgcggctgct
      781 acgccaattg cagcttttgc tgcatcgcgg cctggcaaga acaaagccta ccgggcaagc
      841 gctatcgtct acgcgacatt gaagacgtcg ccgacgagat ggtcgagatg cagcgcgcgc
      901 ggggcatcga cattttcgta ttccacgacg acaacttctt cgtccccggg cacaagaaga
      961 acgcagcgcg cttctccgcg ttggcggacg cgctcgagcg tcggcaaatc ggaccgtttg
     1021 cgacggtagt gaaggcgcgc cccaccgacg tggatccgct cgtgttcgag atcctgcggc
     1081 gtcgattgaa ctgtatccgc gtgtacatcg gcatcgagac cgacgcggac caaggcctcg
     1141 agaccctgcg tcggtggagc caagcgaagc agaacaagcg cgcaatcgag gtggcgcgcg
     1201 cgctcgaact ctacacctgt ttcaacctcc tggtgttcga cccggacacc acgctcgagt
     1261 ctttgcgcac gaacatcgcg ttcatgcgct ttgcttgcga atatccatcg aacttcggac
     1321 gggtcgagct ctacgcgggc acgccgctcc tcgcgcgcat gcaagccgaa gggcgcacca
     1381 gtggcgacta catgcaatgg gactaccggc tggcgagtcc cgagctcgag cgcgtattcg
     1441 agctcgcgat ggactgcttt cacgagcgaa acttcggccc cgaggcgctc aacaaccgta
     1501 tcatgggcac gcgcttcgac gtcgaagtgt gccgccactt ccaccccgac cggttcgacc
     1561 cggcgtggct cgccgaaggc aaatcactca ccagtgaact ttcattggac tcggcgcgcg
     1621 cgctcgaagc gatcgtctct cacgtcgagc agcgcgcgcc gaacgaggac cgcgccttgg
     1681 ttctcgcctt gtcgaagcaa cttcgggcaa ccgagcgcgc ggtgggcgaa gcggcgcgtg
     1741 aactggcgcg caagctgcaa cgcgcagtgg gccgtggcag cccgctcacc gacatcggcg
     1801 accgggttgc aacaccgctt cagactcaac gcctcgccgc gggagccatg ccatgagcaa
     1861 ccgtcgtcgc ctgaagctca tgaacgaggt gctacctagc cccaccaacg ctggcgcttc
     1921 gagcccacgt gcccgcacgc tcctgcacat gcagcggctc ctctcggcga ccgcctttgc
     1981 cgtcgcgtgc tcgaagcaaa ccgcgcccga accaccgggc tacggtgtgg tcgaccccat
     2041 gccgacgccc gcgcgggatc gcgatgagcc cgatgcgagt gcgcccatcc ccgtcccaat
     2101 ctccgacccc aaagtcgacg ggggcaccgc gcaatttgcc gagccgcccc cttcggcgag
     2161 ccagagcgcc aagccgccga agggcaagcc ccccaaacaa ccagagccgc ccggctatgg
     2221 cgtcgtcgat cccatgccgc cgcccgcgaa gcgaaatcgc agtgaaccgt gaccgcgagg
     2281 gagctcgacc atctcgccga gaccagccgc ccgagtcgcc tcgtcgccgt acgctagcgg
     2341 ctcaacgtgt gctcgatgcg cttatccggc acgagccaga tcaaggcgac gagtgcgtac
     2401 agcgcgtcgg agatgaattg gttcacgaac gcgagcagga tcgcgagcgc gtagaagacc
     2461 agcgagattt ttcccttcct atctcggccg accgcgaccg aaagcttcga atgcgggccc
     2521 tgaaccgaaa gaatgacgtt ctgcaagaga gtgtaggaca cggcgctcat cagtaagacg
     2581 aggccgtagg ctgcggtcgg cagcgccgcg aaatggttct cgcccatcca accggtgacg
     2641 aaagggacca gcgacagcca gaacagcagg tgcaggttcg cccagagcac gccgccgctg
     2701 atgtgttccg tcgcgtgcaa catgtggtga tggttgctcc agtagatgcc gaggaacacg
     2761 taactcatca cgtaggtgag aaacacgggg accaagtgtc gaatggcgcc ccagtcggtt
     2821 ccgtgcggaa tcttcagctc cagcaccatg atggtaatca ggatcgcgaa tacgccgtcg
     2881 ctgaaggctt ctagccgtcc tttgctcata ccagctccat tcatgcggcg gtgattctag
     2941 gcgagg
//
LOCUS       DASTKS010000800         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1689381, whole genome shotgun sequence.
ACCESSION   DASTKS010000800 DASTKS010000000
VERSION     DASTKS010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689381"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFQ35_16570"
     CDS             <1..267
                     /locus_tag="VFQ35_16570"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9932321.1"
                     /translation="PEGVVVDIGRASNALANVLSEVEYRNLDEVPAFNGKNTTTEFLA
                     HFVFQRMAERLRAGDLGSGSAEITSMKVTLRESHVASASFEGAL"
     gene            complement(272..571)
                     /locus_tag="VFQ35_16575"
     CDS             complement(272..571)
                     /locus_tag="VFQ35_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9932322.1"
                     /translation="MKSPREVQAIANILGGVPILGVVPGSPAERSGLRYGDIVLSLNG
                     VLTDSFEAFLSAHDLNHGGELAIEVFRDGVRLSLTMKVSSRRRGDKSPPADASLC"
     gene            969..2606
                     /gene="pgm"
                     /locus_tag="VFQ35_16580"
     CDS             969..2606
                     /gene="pgm"
                     /locus_tag="VFQ35_16580"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HET9932323.1"
                     /translation="MSNHPLAGKPAPAELLIDPERLIAAYYSERPDVAEPEQRVSFGT
                     SGHRGSPLSRAFNEAHILAITQAICEYRKQEGIDGPLFLGKDTHAASAPAERTALEVL
                     AANGVETLIQANDGFTPTPAVSHAVLSYNRGRNAGLADGVLVTPSHNPPEDGGFKYNP
                     PNGGPADTNVTAWIQDRANQLLSNGCREVRRVTYEAAMKAPTTRPIDIAEGYIDDLAA
                     VVDVEAIRDAKIRIGVDPLGGASVHYWPRIAERWGLTLDVVNPAVDPRFAFMTVDHDG
                     KIRMDCSSAFAMAKLVALKDRYDVAFGNDPDADRHGIVSSSLGLLNPNHYLAVAISYL
                     FSHRPKWSPKARIGKTLVSSGLIDRVAAALGREVAEVPVGFKWFVPGLISGSLAFGGE
                     ESAGASFLRREGFAWTTDKDGLILCLLAAEMMAVTGKEPGEIFRELTAAHGTQYYTRL
                     DAPATPAEKARLKALSPAAVKAETLAGETIRARLTEAPAGGAIGGLKVTTDNGWFAAR
                     PSGTENVYKIYAESFKSRDHLDLLVKEAKAIVAAALA"
BASE COUNT          505 a          911 c          878 g          467 t
ORIGIN      
        1 cccgagggcg tcgtggtcga catcggccgc gcctcgaacg cgctcgctaa cgttttgtcc
       61 gaggtcgagt atcggaatct ggacgaagtg cctgcgttca acggaaagaa tacgacgacg
      121 gagtttctcg ctcacttcgt atttcaacga atggcagagc ggcttcgcgc gggtgatctc
      181 ggctcgggca gcgccgagat cacgtcgatg aaggtcacgt tgcgcgaatc gcacgttgcc
      241 tcggcgagct tcgaaggcgc gctctgaatc atcagcaaag cgaagcgtcg gccggtggcg
      301 acttgtcgcc gcgacgtcgg ctcgacactt tcatcgtgag cgacaggcgc acgccatcgc
      361 gaaatacctc gatcgcgagc tcgccgccgt ggttgaggtc gtgcgcgctc aggaacgctt
      421 cgaagctatc ggtgagaacg ccgttcaacg agaggacgat gtcgccgtaa cggagaccgg
      481 agcgctcggc tggactaccg gggacgacgc ctaagatggg aacacctccg aggatgttgg
      541 cgattgcttg aacttcacgg ggacttttca tactgatttc ctggcttgcc cgcggtgatt
      601 ggattgggac cgcttgcgtt gcttgcgccg gaactcactt caggaagcgt gccacggttc
      661 gcgcgttgga aaatcgtgat taaattcaat cgcttgaccg tgccgggtga cggcgccgcc
      721 cctcgatcgc tcgtcccgtt cagggaccgc gcgatccccg tctgagactg gtcgggacgg
      781 cgcgctgttg caccgcctag cgcgccatcg agtcgagcgc gcaggggaga gcggggcgaa
      841 aacgcgttcg cgctcacggc gatgctcgac gaaaaaaatc ggcgcctgct ttgctctctt
      901 accgaagcac acctgcgcga ctatggtgcg agcaggtacc tcgagcgcga ggtatcgctc
      961 gaaacgccat gtcgaaccat cctcttgccg gaaagccagc ccccgccgag ctcctcatcg
     1021 atcccgagcg cctcattgcc gcgtattact ccgagcgacc cgacgtcgcc gaacccgagc
     1081 aacgagtcag cttcgggacc agcggacatc gcggctcgcc gctctcgcgc gccttcaacg
     1141 aagctcacat cctcgccatc acgcaggcca tctgcgagta ccgaaagcaa gagggcatcg
     1201 acggcccgct gttcctcggc aaggacacgc acgccgcgtc ggcgccggcc gaacgtaccg
     1261 cgctcgaagt gctcgccgcg aacggggtgg agacgctgat tcaggccaat gacggcttta
     1321 cgcccacgcc ggcggtgtcg cacgcggtac tcagctacaa ccgcggccgc aacgctgggc
     1381 tcgccgacgg agtcctcgta actccttccc acaatccgcc cgaggacggc ggtttcaagt
     1441 acaacccgcc gaacggaggg cccgcagaca cgaacgtcac tgcgtggatc caggatcggg
     1501 ccaatcaact gctctcgaac gggtgccgcg aggttcggcg cgtcacctac gaagcggcga
     1561 tgaaggcgcc caccacgcgc ccgatcgaca tcgcggaggg gtacatcgac gacctcgcgg
     1621 cggtcgtcga cgtcgaagcg attcgcgacg ccaagattcg aatcggcgtc gaccccttgg
     1681 gcggcgccag cgtgcactac tggccgcgta tcgccgagcg ctgggggctt acgctcgacg
     1741 tcgtgaaccc ggctgtggat ccgcgcttcg cgttcatgac cgtcgaccac gacgggaaga
     1801 tccgcatgga ttgctcgagc gccttcgcga tggcaaagct cgtcgcgctg aaagaccgct
     1861 acgacgtcgc gttcggcaac gaccccgacg cggatcggca cggcatcgtc tcttcgtcac
     1921 tcgggctcct gaacccgaat cactacctcg ccgtcgcgat ctcctacttg ttctcgcacc
     1981 ggccgaaatg gagccccaag gctcgcatcg gcaaaacgct cgtgagcagt gggctcatcg
     2041 atcgagtagc cgcggctcta ggccgtgagg tggcggaggt gccggtcggc ttcaagtggt
     2101 tcgtgccggg gctcatcagc ggctcgctcg ccttcggcgg ggaagaaagc gcgggtgcca
     2161 gcttcctccg gcgcgagggc ttcgcgtgga ccaccgacaa ggacggtctc atcttgtgcc
     2221 tactcgccgc ggagatgatg gccgtcaccg gaaaggagcc gggcgagatc ttccgcgagc
     2281 tcaccgccgc gcacggcacc cagtactata cgcgcctcga cgccccggcg actccagccg
     2341 aaaaggctcg cctgaaggcg ctgtctccgg cggccgtgaa ggccgagacg ctggccggag
     2401 agaccatccg cgcgcggctt accgaagcac ccgccggcgg cgcgattggg gggctgaagg
     2461 tcacgaccga caacggttgg ttcgcggcgc gcccctctgg caccgaaaac gtctacaaaa
     2521 tctacgccga gagcttcaag agccgggacc acctggacct cctggtcaaa gaggccaaag
     2581 ccatcgtggc ggctgcgctc gcctgacatt cggctattag cgttgtggcg ttactggcgc
     2641 ccccctggta tcagggcggg cacccatggt agcgcgcgcc ctcatcatcc gtcccgagcc
     2701 agaaccgacg tttcgcgttg gcgggctcgg cctcgtggaa cgttggcaaa agtcgctcct
     2761 c
//
LOCUS       DASTKS010000801         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_573451, whole genome shotgun sequence.
ACCESSION   DASTKS010000801 DASTKS010000000
VERSION     DASTKS010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573451"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFQ35_16585"
     CDS             <1..801
                     /locus_tag="VFQ35_16585"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HET9932324.1"
                     /translation="QQSLLAGRMKLRRMDPSLRNFAVFSHYEGATRYSGEREGDISIV
                     LVPEGWWWVIPMRGDRTSVGLVAPAHTLSGRKPDEAYFNEKLASSEFLKQRFESARRV
                     APVRTISDWSYTSERFAGDRWVLVGDAAAFIDPVFSTGVYLGMIGAFKAVEAILPAFE
                     KRRFSREEFLGYERFVSRGVRTYRDFVRGFYHPAFVEVLLHPSDWLGLRGAITSLLAG
                     WGVESFDVSWRVTLFQLLARINRHVEIVPRLPERRAAHAAHAAHIGAP"
     gene            complement(930..2042)
                     /locus_tag="VFQ35_16590"
     CDS             complement(930..2042)
                     /locus_tag="VFQ35_16590"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9932325.1"
                     /translation="MRVLVSCACSLVIAASFTACKKSEPPTETTAPSSAPVTSAPEVP
                     AGPRADDMKLPTLGAVPIPESNPMSDEKVALGNQLFFDKRLSVDETRSCYSCHQNADG
                     TGGHDPVAVGAKGKQLTRHSPVMWNVGYLPRLYWDGRSDSLEAQGTAAWAGGNMGVGK
                     ENLEAKAKDIAKIKGYKKQFDKVFPGKGATPETIIQAISAYERTLVCDDTAYDKFAKG
                     DKSALNAKQKEGLELFMGKGQCVTCHTPPYFSVAYLGQEGAYFNAGRGIQGKKPEEVD
                     VGRMTVTKKDTDWAAFKPPTLRNVSKSAPYFHDGSEHTLEDAVTYMASGGFDNPHKSP
                     LLADRKLTKAEIQSIVAFLEGLECKGELKEPKLPEK"
BASE COUNT          354 a          740 c          688 g          464 t
ORIGIN      
        1 cagcagtcgc ttctcgccgg tcgcatgaag ctccgccgca tggatccgag cctgcgcaac
       61 ttcgcggtgt tctcccacta cgagggagcg acgcgctact caggcgagcg tgagggcgac
      121 atcagcatag tgctcgtgcc cgagggttgg tggtgggtga tcccgatgcg cggcgatcgc
      181 acgagcgtcg ggctcgtggc gccggctcac accttgagtg gccgtaagcc ggacgaagca
      241 tatttcaacg agaagctcgc gagctccgag ttcctgaagc agcgtttcga gtccgcacgc
      301 cgggtcgcgc cggttcggac catttcggat tggtcgtaca cgagcgagcg cttcgccggg
      361 gatcgctggg tcctcgttgg cgacgccgcc gcgttcatcg accccgtttt ctcgactggc
      421 gtctacctcg gcatgatcgg cgccttcaaa gcggtcgaag cgattttgcc ggcgttcgag
      481 aagcgccgct tctcacgcga agagttcttg ggctacgagc gcttcgtgtc tcgcggcgtg
      541 cgcacctatc gagacttcgt gcgaggcttc tatcaccccg ccttcgtcga agtcctgctg
      601 catccgagcg attggcttgg gctccgtggc gcgatcacct cgttactcgc gggttggggc
      661 gtcgaaagct tcgacgtcag ctggcgtgtc acgctgttcc agctcttggc tcgcatcaac
      721 cgccacgtcg aaatcgttcc gcgcctcccc gagcgccgcg ccgcgcacgc cgcgcacgcc
      781 gcgcacattg gcgcgccctg agaacgcgtc tgcacgccgc gcacgttggt gcgccctgaa
      841 aacgcgccgc gcgccagtcc gcgcgccctg aaagccccag ccccgatgag cgacgctcgg
      901 cgccgctcac gcggtgctga cgcgaacgct cacttctccg gcagcttcgg ctccttgagc
      961 tcgcccttgc attcgaggcc ctctaggaac gcgacgatcg actgaatctc ggctttggtc
     1021 agcttgcggt ccgcgagcag tgggctcttg tgcgggttgt cgaagccgcc gctcgccatg
     1081 tacgtcaccg catcttctag cgtgtgctcg gagccgtcgt ggaagtatgg cgcgctcttc
     1141 gatacgttgc gcaacgtggg gggcttgaaa gctgcccagt cggtatcctt cttcgtgacg
     1201 gtcatgcgcc ctacgtcgac ctcctctggc ttcttgcctt ggatgccacg cccagcgttg
     1261 aagtaggcgc cctcttgacc gagatacgcg acggagaaat agggcggggt gtgacatgtg
     1321 acgcactgcc cctttcccat gaagagctcg agcccttcct tctgcttcgc gttgagcgcg
     1381 ctcttgtcgc ccttcgcgaa cttgtcgtac gccgtgtcgt cacaaacgag cgtgcgttcg
     1441 tatgcggaaa tcgcttgaat gatcgtctcc ggggtggcgc ccttacccgg aaacaccttg
     1501 tcgaactgct tcttgtagcc cttgatcttg gcgatgtcct tggccttcgc ctcgaggttc
     1561 tccttgccga cacccatgtt gccgcccgcc cacgcggccg tgccttgcgc ctcgagcgag
     1621 tcggaccgcc cgtcccaata tagacgtggc aagtagccga cgttccacat cacggggctg
     1681 tggcgcgtca gctgcttacc tttcgcgccg accgcaacgg gatcgtgccc tcccgttcca
     1741 tccgcgtttt gatggcacga atagcaagag cgcgtctcgt cgacactcaa gcgcttgtcg
     1801 aaaaatagct gattcccgag agcgaccttc tcgtcactca tcgggttgct ctccggaatc
     1861 ggaacggctc cgagggtcgg aagcttcatg tcgtcggcgc gcggcccagc aggaacctcg
     1921 ggtgccgacg tgacaggcgc cgaactcggc gccgtcgttt ccgtcggtgg ctccgacttt
     1981 ttgcaagcgg tgaaggatgc ggcgatgacg agcgagcaag cgcagctaac gagaaccctc
     2041 atgtcctcgt ggatagtcga ccctccgagg cgacgcaatg ggccgtatgg cacgcaagtt
     2101 ttcgcatgac ccccgaatga aacgaacttt cgctgctcgt atcgaaccgc gcgtgaaatg
     2161 cgaaccgatt acttcagcca gtcgcaggcg acgcccagtc cgtcgccttc tcgcgtcgag
     2221 cgtcgagcgt cgagcgtcga gcgtcg
//
LOCUS       DASTKS010000802         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1244203, whole genome shotgun sequence.
ACCESSION   DASTKS010000802 DASTKS010000000
VERSION     DASTKS010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244203"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFQ35_16595"
     CDS             complement(<1..494)
                     /locus_tag="VFQ35_16595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9932326.1"
                     /translation="MSESAPDRIYTLVAELSYRCPLRCPYCSNPLDYNERRDELTTAQ
                     WLSVLDQAAALGALQVTFTGGEPLLRRDLEELVSRAHALDLYSSLITSGLPLGRERLA
                     ELKRCGLTAVQLSLQDPREANSNRIAGTDCHAQKLEVASWIKELGFPLTLNVVLHREN
                     LDQVA"
     gene            complement(491..775)
                     /gene="pqqD"
                     /locus_tag="VFQ35_16600"
     CDS             complement(491..775)
                     /gene="pqqD"
                     /locus_tag="VFQ35_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HET9932327.1"
                     /translation="MIAPSARPKLAQKVKLRLDPKTRKHVLVYPEKGLELNATGGAIA
                     SLCTGEFTFEQIVQRLKEQFQQGDTAALEAHVSAFLSALAERGLVEGLEL"
     gene            complement(831..1556)
                     /gene="pqqC"
                     /locus_tag="VFQ35_16605"
     CDS             complement(831..1556)
                     /gene="pqqC"
                     /locus_tag="VFQ35_16605"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HET9932328.1"
                     /translation="MSDVDPTRPPLPREEFVEWLRREGATRYHDAHPVHVAMHEGKLS
                     KEELQCWVLNRYYYQTRIPIKDALILSKSEDPAFRRMWIHRIHDHDGNEAGEGGLELW
                     LRLADGVHLDRELTASCRAVLPGVRFACDAYVELVRERSLLEAVASSLTEFFAPDIMS
                     KRIAAWERFYPWVKEETLAYFRSRVTRARRDSLEAIEYVVKHANTYALQERCVAALIR
                     KTEILWHLADCVQLEIERQRSRK"
     gene            complement(1553..2470)
                     /gene="pqqB"
                     /locus_tag="VFQ35_16610"
     CDS             complement(1553..2470)
                     /gene="pqqB"
                     /locus_tag="VFQ35_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HET9932329.1"
                     /translation="MFVRVLGSSAGGGFPQWNCGCPNCTGVRAGTIRAKARTQESVAV
                     SADGDSWFLLNASPEIRAQIEGFPRLHPRALRDTPLAGILFTSGDLDHCLGLLSLRES
                     QRLDVYATAAMRRSFVEGNTLYPTLERFEGQVRWHPLAPNERCALLLSDGKESGLELV
                     PYSVRGKPPLHAKQLAPSPGDNLGLRIEDRRTGGVLAYVSGVTGPSSELEACVAGANA
                     IFFDGTFWTRDELVALGVSNRYAEDMAHWPLSGPDGSLAFLSRQPAARKLFIHINNTN
                     PILREDSPERAEVMARGVEVSEDGQEFSL"
BASE COUNT          497 a          858 c          861 g          488 t
ORIGIN      
        1 gcaacttgat cgaggttctc gcgatggagc acgacgttga gcgtgagcgg gaacccgagc
       61 tctttgatcc agctcgctac ttcgagcttc tgagcgtggc agtcggttcc tgcgatgcgg
      121 ttcgagttcg cctcccgagg gtcttgaagc gagagctgca ccgcggtcaa gccgcagcgc
      181 ttcaactcgg caagccgctc gcgcccgaga ggcaagccgc tcgtgatgag cgaggagtac
      241 aaatcgaggg catgcgcgcg gctgaccagc tcctccaaat cgcgccgtaa gagcggctcg
      301 ccacccgtga acgtcacctg cagagcgccg agggcggcgg cctgatccag aactgaaagc
      361 cactgcgcgg tcgtgagctc gtctctacgt tcgttgtaat cgagcggatt cgaacagtag
      421 gggcagcgca gagggcagcg gtacgatagc tcggcgacga gggtgtaaat gcgatctgga
      481 gcggattcgc tcacagctcg aggccctcga cgagtccgcg ctcggcgagc gcagagagaa
      541 aagcggacac gtgggcttcg agcgccgccg tgtcgccttg ttggaactgc tctttcaggc
      601 gctgaacgat ttgttcgaac gtgaactcac ccgtgcacaa gctcgcgata gctccgcccg
      661 tggcgttgag ctcgagcccc ttctcgggat agacgagcac gtgctttcga gtcttcgggt
      721 cgaggcgaag cttcaccttc tgcgcgagct tggggcgcgc gctaggcgcg atcacttgcg
      781 gccccgctgg cgctcgattt cgagctgaac acagtcggcg ctcggcgcga tcacttgcgg
      841 ctccgctggc gctcgatttc gagctgaaca cagtcggcga gatgccacag gatctcggtc
      901 ttgcggatca gcgcggccac gcatcgctcc tggagggcgt acgtgtttgc gtgcttcacc
      961 acgtattcga tcgcttcgag cgaatcgcgc cgcgcccgcg tgacgcgcga acggaagtac
     1021 gcgagcgtct cctctttgac ccatgggtag aagcgctccc acgccgcgat gcgcttgctc
     1081 atgatgtcgg gcgcaaagaa ctcggtcagc gacgaagcga ccgcctcgag cagcgagcgc
     1141 tccctgacga gctcgacata ggcgtcgcac gcaaagcgca cgcccggtag cactgcgcgg
     1201 cagctcgccg tgagctcacg atcgagatgc acgccgtcgg ccaagcggag ccagagctcg
     1261 agacctccct cacccgcctc gttgccgtcg tgatcgtgga tgcggtgaat ccacatccgc
     1321 cgaaacgccg gatcctccga tttagagagg atcaaggcgt ctttgatggg gatgcgcgtc
     1381 tggtagtagt agcggttcag cacccagcac tggagctcct ctttcgagag cttgccctcg
     1441 tgcatcgcaa cgtgaaccgg gtgcgcgtcg tggtagcgag tcgcgccttc gcgtcgcaac
     1501 cattcgacga actcttcgcg cgggagtggg gggcgcgtgg gatcgacgtc gctcatagcg
     1561 aaaactcctg gccgtcctcg gacacttcca ccccgcgcgc catcacctcg gcgcgctcgg
     1621 gcgagtcttc tcgcaaaatg gggttcgtgt tgttgatgtg gatgaagagc ttgcgcgccg
     1681 cgggctgcct gctcaaaaac gcgaggctcc cgtcgggacc gctgagcggc cagtgcgcca
     1741 tgtcttcggc gtagcgattg ctcacgccga gcgcgacgag ctcgtcgcga gtccaaaacg
     1801 tcccatcgaa gaagatcgcg ttcgcgccgg cgacgcacgc ttcgagctcc gaggacggcc
     1861 cggtgacgcc tgaaacgtac gccagaactc cgcccgtcct tcggtcctcg atgcgcaaac
     1921 cgaggttgtc gcctgggctc ggagcgagct gcttcgcgtg tagcggtggc ttgccgcgca
     1981 ccgagtaggg tacgagttcg agcccgctct ctttgccatc ggacagcagc agcgcgcagc
     2041 gctcgtttgg cgcgagtggg tgccagcgta cttgcccttc gaagcgctcg agcgtcgggt
     2101 acagcgtatt gccttcgacg aagctccgac gcatggccgc ggtcgcgtag acgtcgagcc
     2161 gctgagactc gcgcaaggac aggaggccca ggcagtggtc gagatctccg ctcgtgaaga
     2221 ggatgcccgc aagcggcgta tctcgcagcg ctcgcggatg cagacgcggg aacccctcga
     2281 tctgtgctcg gatttctgga gacgcgttca gcaggaacca gctgtccccg tcagcactga
     2341 cggcaactga ttcctgcgta cgcgctttgg cgcggatggt acctgcacgg acgccggtgc
     2401 aattagggca accgcagttc cactgcggaa agccgccacc ggccgatgac ccgagcaccc
     2461 ggacgaacac gtagcgttac ctttccggat gccgaggggt gcggttcaga tcccgtcgaa
     2521 gtcttcttgg tacgagccga tctctgcgtt catctcaatt tcaacgaaag acggtagttc
     2581 ccaaaccatg atgaaatctc cgacaggaag caggtaacga gcgagaatct cgatatccca
     2641 cgcgcactct caccggtcaa gcaacacccc gccgcccaga aagcctgagt ccccaacaac
     2701 cctc
//
LOCUS       DASTKS010000803         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_796975, whole genome shotgun sequence.
ACCESSION   DASTKS010000803 DASTKS010000000
VERSION     DASTKS010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796975"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VFQ35_16615"
     CDS             complement(<1..1045)
                     /locus_tag="VFQ35_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932330.1"
                     /translation="MRRITAIVLSSTALLLACTAEQDSAEDTTASQGSPLTPASQSGL
                     KVDDMLLAMAQDGSGVLSLALDANEQKRVSRENQGATFSLSANPKEKAIVFNDLGKAP
                     DAKAGDGLFTASIPLSIEKLTALNREIAQLKSGPLYRFAPGSRLVSRREEFSQTNAID
                     LTALKRGATIRLPILAELGPLKTLGAVTTASINPQKSLLIRSLPVIEDPTRTMNPCDT
                     SASNTPLKKWTFGHLMEQMAQGSGLTPSAFVEQWLNSWRTQQIVNASGGGPVLDATSA
                     AALANIDSLIINPWRQRSGGGALDLSIAPFRLQAIVYRSDLAQSSPYGGSTKGNNAGE
                     LRFVFGAMEVRDQN"
     gene            1785..2876
                     /locus_tag="VFQ35_16620"
     CDS             1785..2876
                     /locus_tag="VFQ35_16620"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET9932331.1"
                     /translation="MAKSRVAVLKVKPETILDDIERLCDLADLSSSLARGATTILKDN
                     ISWHFPFPAANTTPWQLEGAVLALKKAGFSELCCVQNETVVTNAFKGEDLNHYKPVFR
                     RHEVPVRFNFREADMKWIEYRPKARLRALHRIYPEGLMLPDYFFGKNIVHLPTMKCHI
                     YTTTTGAMKNAFGGLLSAKRHYTHSVIHQTLVDLLAIQKEIHGGVFAIMDGTTAGNGP
                     GPRTMIPVVKDYMLASADQVAIDAVAAKMMGFDPLSIEYIRLAHEDGLGICDPRDIEI
                     VGADISGENWGFQVGDNAASRVGDIVWFGPLKAAQKLFFHTPLVHAFVLGSELYHDHL
                     RWPRKEQKLFEEWQRGTGWGRLFASYASL"
     gene            complement(2873..>3703)
                     /locus_tag="VFQ35_16625"
     CDS             complement(2873..>3703)
                     /locus_tag="VFQ35_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9932332.1"
                     /translation="RAKALLRRRDPSVASWADSHQEHLRTMDTVEKELSRVGVQAMWI
                     SRAHAIFDASDAAMVIALGGDGTLLAASHSVESAPILGVNSAPKHSVGFFCAARRETF
                     AKLFAEAMEGRLSGVTLTRMRVSLNGRLRSSRVLNEALYAHDSPAATSRYVLELGKIR
                     EDQRSSGFWIGPAAGSTAAQHSAGGRVLPLASKKLQLVVREPYEPHGQRFRMRTRLIT
                     PSEHLRVHSKMDEAKLWLDGPNREISVRLGDIVEFGVSDEPLRVLGLGTKSRPKVGRG
                     "
BASE COUNT          647 a         1180 c         1187 g          689 t
ORIGIN      
        1 tgttttgatc gcgcacttcc atcgcgccga acacgaagcg gagctcaccg gcgttgttgc
       61 ccttggtgga gcccccgtaa ggcgagctct gcgcgagatc ggagcggtac acgatcgcct
      121 gcagccggaa cggcgcgatg ctgagatcga gcgcgccgcc tccactccgc tggcgccagg
      181 ggttgatgat caagctgtcg atgttggcca gcgccgcggc gctcgtggcg tcgagaacgg
      241 gaccgccccc gctcgcgttc acgatctgct gcgttcgcca ggagttcagc cattgttcga
      301 cgaacgcgct cggggtgagg cccgaacctt gcgccatctg ctccatcaag tgaccgaagg
      361 tccacttctt caacggcgtg ttgctcgcgc tcgtgtcgca cgggttcatc gtgcgcgttg
      421 ggtcttcgat cacgggtagc gagcgaatga gcagcgactt ctgagggttg atgctggcgg
      481 tggtgaccgc gcccagcgtc ttcagcgggc cgagctcggc gaggatcgga agccgaatgg
      541 tcgcgccgcg cttgagagcg gtgaggtcaa tcgcgttcgt ctgtgagaac tcttcgcgac
      601 gcgacacgag acgggagccg ggggcgaatc ggtagagcgg tccgctcttc agctgcgcga
      661 tctcgcggtt tagcgcggtg agcttttcga tgctgagcgg gatgctcgcc gtgaacaggc
      721 catcaccggc ctttgcgtcc ggcgccttgc ccaagtcgtt gaatacaatc gctttttcct
      781 tggggttggc gctgagagag aaggtcgcgc cctggttctc ccgagacacg cgcttctgct
      841 cgttcgcgtc gagggcgagc gagagcacac cgctcccgtc ctgagccatc gcgagcagca
      901 tgtcgtccac cttgaggcct gactgcgacg cgggcgtgag cggtgaacct tgcgacgcgg
      961 tcgtgtcttc ggcggagtcc tgctcggccg tacacgccaa gagcagcgcg gtggaactca
     1021 agacgatagc agtgattctt ctcatgacgt ttacctcatc gttcttgctc gcggtgcgag
     1081 cggacacgac accgaggaca accctcggcg tcgtgcggtg gtgagcgcga gcacgtgagt
     1141 cgcatggtct atcacgatga gtccattgcg ttcgattaca tgagtgggtg ttgatcgaat
     1201 gaacgagttt cgccgatctc caccacccat gtgagtttgg tcgcgaacgt ggcgaagtcc
     1261 gtgtggcatt cgagttacgc gcgtcgtcgc cgagcgttca cacgacgatc tcgagcgcgg
     1321 tcgaacatgg tcgaaatggc gcgaatcgag cgtgtttggc gatcgggcgg cgccgctcgc
     1381 cgcgctcgca ctttctcttc gtgcagtgaa gagcggcgag tttcgcgcgc gcacgggagt
     1441 cgttgtctta cttctcgatc gcgtcgcgat gcttttcgat ttgttaacga agcgcactga
     1501 ggtagtaggt ttgccgcgca tgacgatgct tttgcgagca ggcgcgcgtc atcgacgacc
     1561 acgggtcgaa cccaaacctc aaacctcgct accaccgagc cgaaagccct tggtccgaag
     1621 gttcgaagct cgagcgaagg aacggccgcg gcaattcgcg ctcaaagcac tgcgtttcgg
     1681 gcctcgaccg gagaccgcct ttgccgctcg ttgccggcca acccacccgt ttgtgctccc
     1741 gcgcttggca gccgctccgg gcgccgccat cctctctcgt acgcatggca aagtcgcgcg
     1801 tcgcggtact caaggtcaaa ccagaaacga tcctcgacga catcgagcgc ctctgtgacc
     1861 tcgcggatct gtcgagctca ctcgcgcgtg gtgcaacgac gatcctcaaa gacaacatct
     1921 cgtggcattt cccgttccca gccgcgaaca cgacgccgtg gcagctcgaa ggcgccgtgc
     1981 tcgccctgaa gaaggctggg ttctcggagc tctgctgtgt gcaaaacgag acggtggtta
     2041 ccaacgcctt caagggcgaa gacctcaacc actacaagcc cgtgttccgc cgccacgagg
     2101 tgcccgtgcg cttcaacttc cgcgaagcgg acatgaagtg gatcgagtac cgacccaagg
     2161 cgcggctgcg ggcgctacac cggatctacc cggaagggtt gatgctcccc gactacttct
     2221 tcggcaagaa catcgtgcat ctgccgacca tgaagtgcca catctacacc acgacgacgg
     2281 gcgcaatgaa gaacgccttc ggcggtcttc tcagcgcgaa gcgccactat acgcactccg
     2341 tgatccacca gactctggtc gaccttttgg cgattcaaaa ggagatccac ggcggggtct
     2401 tcgcgatcat ggacggcacc accgccggga acgggcccgg tccgcgcacg atgatcccgg
     2461 tggtgaagga ctacatgctg gcgagcgccg accaggtcgc gatcgacgcg gtcgcggcca
     2521 agatgatggg cttcgatccg ctgagcatcg agtatattcg gctcgctcac gaggacggct
     2581 tggggatttg cgacccgcgc gacatcgaga tcgttggcgc ggacatttcg ggagagaatt
     2641 ggggatttca ggtgggggac aacgctgcga gtcgcgtggg cgacatcgtg tggttcggtc
     2701 cgctcaaggc cgcgcagaag ctcttcttcc acaccccgct cgtgcacgct ttcgtgctcg
     2761 gcagcgagct ctatcacgat cacctgcgct ggccgcgcaa ggagcagaag ctattcgaag
     2821 agtggcagcg cggcaccggc tggggacgcc tcttcgcgag ctacgcgagc ctctagccgc
     2881 gcccgacttt gggacggctc ttggtcccga gccccaatac gcggaggggt tcgtcggaga
     2941 cgccgaactc cacgatgtcg ccgaggcgca ccgaaatttc gcggttcgga ccgtcgagcc
     3001 agagcttcgc ctcgtccatc ttgctgtgga cgcgcaggtg ctcgctcggg gtgatcaggc
     3061 gcgttcgcat gcgaaagcgc tgaccgtgcg gctcgtacgg ctcgcgcacg acgagctgaa
     3121 gcttcttcga agcgagcggc agcacgcgac cgcccgccga atgctgcgcc gccgtcgagc
     3181 ccgccgccgg cccaatccaa aaaccgctcg agcgttggtc ctcgcggatt ttcccgagct
     3241 cgagcacgta gcgcgacgtc gccgcgggag agtcgtgcgc gtagagcgcc tcgttcagca
     3301 cgcgcgacga ccgaagccgc ccgttcaagc tcactctcat gcgcgtcagc gtgacgccgc
     3361 tgagccgccc ttccatcgcc tccgcgaaga gcttcgcgaa cgtttcgcga cgcgcggcgc
     3421 aaaagaagcc cacgctgtgc ttgggcgcgc tgttcacccc caaaatcggc gccgactcga
     3481 cgctgtgcga agcggcgagt agggtcccgt caccgccgag cgcgatcacc atggccgcgt
     3541 cggaagcgtc gaagatggcg tgcgcgcgcg agatccacat cgcttgaacg ccgacacgcg
     3601 agagctcctt ctccaccgtg tccatcgtgc gcaagtgttc ttggtgtgag tccgcccacg
     3661 aagcgacact cgggtcgcgg cgccggagca gcgccttcgc gcg
//
LOCUS       DASTKS010000804         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1468300, whole genome shotgun sequence.
ACCESSION   DASTKS010000804 DASTKS010000000
VERSION     DASTKS010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468300"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..398
                     /locus_tag="VFQ35_16630"
     CDS             87..398
                     /locus_tag="VFQ35_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932333.1"
                     /translation="MNKRLAVLEKMTSSDAADSFAFYGLAMEYRKEGRIDDALATFQR
                     LKAKDPGYLPMYLMAGQMLVEVRRATEARAWLEAGIDVATARGDTKTLGELESELANA
                     R"
     gene            complement(424..720)
                     /locus_tag="VFQ35_16635"
     CDS             complement(424..720)
                     /locus_tag="VFQ35_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932334.1"
                     /translation="MLRRCEICQARGESATSTGKLRRLLVGQRVVALCMHHAEAAESF
                     EASTIDQLRALFVEQNGRRSLLDRRSPLDRRIFPARPEGRRGAAGRRSSDERSG"
     gene            809..2608
                     /locus_tag="VFQ35_16640"
     CDS             809..2608
                     /locus_tag="VFQ35_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932335.1"
                     /translation="MLSYWTRASLLSTCLVLGCAPAPSQSAPKAPTSAASPSRPPNPF
                     ETTLDWKPEQTTAPAGFWFRIKSPNAILPRLVKLAADPTLRGFSDLRALAGITLGGEL
                     SALVDLDAPITVVMARDANDFTVVAAAHPSNGAQFAPEKLGDKLKPLGPARWEIEPGD
                     GGEKFHCELWHVAAPVGYRVLCGLKGTDFAAHAPFLLGQLEKTPPSADATVGSSHFPL
                     AESIAKQQRERAADSEANVESESIGRWLQSFAKAESVAFDLDFVHDDVELAVEFDYAE
                     ADTSPAVRAWITKTPEPLPPQFWQTLDVSPVALASAGVDEATMKDLLSSPVVSELLAS
                     LNRANGVTPEVATEMLNAMGGLIPNRLRFTMSMGGTLPSSTRQGEKRASGPFKSSKAA
                     NGWMLLGYAGSGERYLKGLAVLEQASKAKHRSANSNAAAPRWTRLAKVPGDLPPSSVI
                     FRQDHAGKSTWFTCVLPGTDWIWILSAASEQQMLAQVRRVLPSLDAPAPRDDTEHAAF
                     GAAPPLMMMSSSLAALKEVSGSSMHDLELSTLPFGGSSRILVGVTGKTKQVAGKAVVT
                     LRVASRWSPPAMADMVELFKRTKSESERAKGSE"
     gene            2803..3201
                     /locus_tag="VFQ35_16645"
     CDS             2803..3201
                     /locus_tag="VFQ35_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932336.1"
                     /translation="MKSRAPSDGTNAIAGAIAAQSDVVANGCAECGLSSAELAIWSTP
                     TLVQDEAEAQTVVSAKPPTFSLSAHGTYRQPVPPGTYLFCATECVAVTVTLGMVTTVN
                     IMQVFGPTSYRVFEAAASMWQTPVPFKVMR"
BASE COUNT          666 a         1167 c         1150 g          611 t
ORIGIN      
        1 gcgagggaac atggctcaat ggacactggg caaaggccta ccttggcggg gccctcccag
       61 catccgtgtg atacgacatc cccgccatga acaagcgcct agcagtgctg gaaaagatga
      121 ccagcagcga tgctgcagat tccttcgcgt tttacggact cgccatggag taccgcaagg
      181 agggtcgcat cgacgacgcg ctcgcgacct tccaacgtct caaggcaaaa gaccctggct
      241 acttgccgat gtacctgatg gcggggcaga tgctcgtcga agtgcggcgt gcgactgaag
      301 cgcgtgcgtg gctcgaggca ggcatcgacg tcgcgaccgc gcgcggtgac acgaaaaccc
      361 tgggtgagct cgaatccgag ctcgccaacg cgcgctgatc cagcgcacgc ggtctcgtcg
      421 acttcaaccc gagcgttcgt cgctcgagcg gcgacccgcc gcgccacgcc tgccctcggg
      481 gcgagccgga aagatgcgtc gatcgagcgg cgagcgccga tcgagaagag agcgacgacc
      541 gttttgctcg acgaacagcg cgcggagctg gtcgatggtc gaagcttcga acgactctgc
      601 cgcctctgcg tggtgcatgc aaagggccac gacgcgctgg ccgaccagca gtcgtcgcag
      661 ctttccggtg gatgtcgccg actcaccgcg agcttggcaa atttcacaac gccgcagcat
      721 ctctttcgcc tagctcgctc gagcacacag ggccaagttt cgaggcacgc ggtgccggcg
      781 gagccgcccc ctggtagctt tccggccgat gctctcctac tggacgcgag cgagcctgct
      841 ctcgacatgt ttggtgctcg gatgcgctcc tgcgccctct cagagcgcgc cgaaggcgcc
      901 gacatccgcc gccagcccga gtcgtccgcc gaatccgttc gagacgacgc tcgactggaa
      961 acccgaacag acaactgctc ccgcggggtt ctggtttcgg atcaagtcgc cgaacgcgat
     1021 cctgccccgc ctcgtcaagt tggcggcaga tccaacgctg cgcgggttct ccgatttgcg
     1081 cgcgctcgct ggaatcactc tcggtggcga gctgtcggcc ttggtcgacc tcgacgcgcc
     1141 aatcacggtc gtgatggcgc gcgatgcgaa cgacttcact gtcgtagccg ccgcacatcc
     1201 ttcgaacgga gcacagttcg ccccggagaa gctcggcgac aagctcaagc cattggggcc
     1261 cgcacgctgg gagattgagc cgggcgacgg cggagagaag ttccactgcg agctctggca
     1321 cgtcgctgcg ccggtcgggt accgcgtctt gtgcgggttg aaaggcaccg acttcgcggc
     1381 gcacgcgccc ttcttgctcg ggcagctcga gaaaacgccg ccaagtgccg acgcgaccgt
     1441 cggaagctcc cacttccccc tcgccgagag catcgccaag cagcaacgag agcgagcagc
     1501 ggactccgaa gcaaacgtcg aatcggaatc catcggtcga tggctccagt cgttcgcgaa
     1561 ggccgagagc gttgccttcg acctcgactt cgtacacgac gacgtcgagc tcgcggtgga
     1621 gttcgactac gccgaagcgg acacgtcccc cgcggtgcga gcctggatca ccaagacgcc
     1681 ggagccgctc ccgccgcaat tctggcaaac gctcgacgtg tccccagtcg cgctggccag
     1741 cgctggcgtt gacgaagcga cgatgaagga cctcctgagc agccccgtcg tcagcgaact
     1801 tctagcgtcc ctgaatcgcg cgaacggcgt aacgcccgaa gttgcgaccg aaatgttgaa
     1861 cgcgatgggc ggactcattc cgaatcggct ccgcttcacc atgtccatgg gaggcacgct
     1921 gccttcgtct actcgccagg gcgagaagcg cgcgtcgggt cccttcaaat cgagcaaagc
     1981 cgcgaacggc tggatgttgc tggggtacgc aggttcgggt gaacgctact tgaaagggct
     2041 cgccgtactc gagcaggcga gcaaagccaa acatcgcagc gcgaactcga acgcggcggc
     2101 ccctcgctgg acacggctcg ccaaagtgcc gggcgatctt ccgccgtcta gcgtcatctt
     2161 tcggcaggac cacgcgggca agagcacttg gtttacctgc gtgctgccgg gcaccgattg
     2221 gatttggatt ctgtccgcag cgagcgagca gcagatgctg gctcaagtgc ggcgcgtgtt
     2281 gccatccctc gacgcgcctg cgccccgcga cgacactgag catgcggcgt ttggcgctgc
     2341 accgccgctc atgatgatga gctcgagcct agcggctctg aaggaagtgt cgggctcgtc
     2401 gatgcacgac ctcgagctgt ccacgttgcc gttcggcggc tcctctcgca tcctcgtcgg
     2461 cgtgaccggc aaaaccaagc aagtggcggg aaaagccgtc gtgacgctgc gcgtcgcgtc
     2521 ccgctggtcg ccgccggcaa tggccgacat ggtcgagctc ttcaagagaa cgaagagcga
     2581 atcggaacgg gcgaagggct ccgaatgaac tcggtccgcg tctccgatcg accgcattca
     2641 cgacacagcg cggctgcgcg tcgatggcgc tcgacggggc gaatggcgag tgtcccccgc
     2701 tcgtgatatc gaaacagcat gcggggtgtt gtttcaatct tggcttgtac ggcgctgctg
     2761 gcgtcggctt gcggaacctg cgatcgggaa gggtgcgata aggtgaagtc gcgcgcaccg
     2821 agcgacggca cgaacgcgat cgcgggggcg attgccgcgc agtcggacgt cgtcgcgaac
     2881 ggctgcgccg aatgcgggtt gagctctgct gaactcgcga tttggtctac ccccacgctc
     2941 gttcaagacg aggccgaagc gcagacggta gtctctgcga aacctccgac gttctcgcta
     3001 tcggcgcacg gaacctatcg acaaccagtt cctcctggca cgtacctctt ttgcgcaacg
     3061 gagtgcgtgg ccgtaactgt caccctaggc atggtgacga cggtgaacat catgcaggtc
     3121 ttcgggccca cttcgtaccg cgtgttcgag gcagcggcct cgatgtggca gacgccggtg
     3181 cctttcaagg tcatgcgctg aggcgaacac gccgctcggc tgcggcccgc aacgccgaaa
     3241 cgccgctgtg acagcgtgat gcagccgagc atgtgaacgc accgaagccg ggcccgtcgc
     3301 cggtttcggc gtacggcgcg ctggattgac ctcttgctcg acgaaaccag cgtgcagtcg
     3361 ttcaactcca ccccattcgc tgacgcagcg gagctcttcg cattctcggc acgctcggca
     3421 gacgcgcccg aaggcgcggt gcgtctacga gtgaactcgg cacgatcgta agcatcgaca
     3481 cagtgcgcgt tcgctcgaga ttcgttgcga cgcacgtagc aagcgctcgg cgccgcgcgc
     3541 gccagtgagc aagtgagcga gcacgaccga aaaaacgact attgctgagt ttcg
//
LOCUS       DASTKS010000805         6996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1468355, whole genome shotgun sequence.
ACCESSION   DASTKS010000805 DASTKS010000000
VERSION     DASTKS010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468355"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFQ35_16650"
     CDS             complement(<1..504)
                     /locus_tag="VFQ35_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9932337.1"
                     /translation="MATDTAPTGPFDARIPNAPLAEMWTTYKSTCKLVNPANKRHLDV
                     IVVGTGLAGASAAATLGELGYNVKTFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVY
                     RLFYETIKGGDYRAREANVHRLAEVSTAIIDQCVAQGVPFAREYGGMLDNRSFGGTQV
                     QRTFYARG"
     gene            complement(514..1287)
                     /locus_tag="VFQ35_16655"
     CDS             complement(514..1287)
                     /locus_tag="VFQ35_16655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HET9932338.1"
                     /translation="MGQTLQTPHGANIAPRATDAPLMRVGSWFEDIGMVSSSVGRKFV
                     MAITGLFLISFLVVHLSGNLLLFKDDGGAAFNAYAHFMATNGVVRLMEAVLFAGIIFH
                     FAEGVALVLKNRAARPKTYELKGDNGATWSSRFMGALGGIITVFLVIHLADFFYKSRI
                     ASPESGGIQVIDAEGTRNMYAAVRESFSSGLYSAVYVVCMVALAYHLLHGFRSAFQTL
                     GLSHRGYALQIRRAGAAFSVIVPLGFAIMPIYFYLRSLN"
     gene            1383..1997
                     /locus_tag="VFQ35_16660"
     CDS             1383..1997
                     /locus_tag="VFQ35_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9932339.1"
                     /translation="MTVRLLMLNGSLRGTSGNTEELLSLAASYVGGDVTVERIALAEY
                     EGSVENLAARLAASDAFLFGTGVYWGSWGSPLQRFLEVMTSYELTPSFLGKPAAVVVN
                     ADSLGASDVAHRLQGVLNHFGCWSPPLASVALTRVAAAVKGRAGFEDVFQVDDMRIAL
                     ENLCRAARIPSPQWSAWSIARTPSVSGAYPGVGPVLAGLPKSTL"
     gene            complement(2034..5636)
                     /locus_tag="VFQ35_16665"
     CDS             complement(2034..5636)
                     /locus_tag="VFQ35_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9932340.1"
                     /translation="MSDSRYQFWIDRGGTFTDIVARGPDGSCRSIKLLSDNPEQYTDA
                     ALAGMRQLLGLGAGERLTVDHVEAVKMGTTVATNALLERRGEPVALFITRGFRDQLRI
                     GYQNRPRLFARHIELPEPLYAQVFEVDERLDATGAVVRPLDENTLELALAEARSAGFR
                     SCAIVFMHAHSFPRHELRALELARKAGFEQISASHRVSPLSKLVSRGHTTVADAYLSP
                     VVRRYVERMANELGDVPLYFMKSNGGLSEARAFQGKDAILSGPAGGIVGMVRTALGAG
                     FDRVIGFDMGGTSTDVSHFSGKDESDCERSFETRIAGVFVRAPMLAIHTIAAGGGSVL
                     HFDGHSYRVGPDSAGANPGPRCYRRGGPLTVTDCNVLLGRIQPSAFPRVFGPHANGEL
                     DRDGVTAGFESLCEEITQCTGQSQTREAVAEGFLRVAVNNMANAIKFISVQRGYDLAD
                     YVLCCFGGAGGQHACLVAEELGIRRIFVHPLASVLSALGVGLARITAIQERAVERTLS
                     AEAQPRLVDEVAELVEAASAEVASQSVDLEAIETRARAHLRYAGTDTALVVSFAEPLE
                     MRLEFERLHRARFGFVMSGRELVTEAISVEARGEGEPPLTATSNRFEVEASGSAPMWV
                     MGRWARVPVYPSAALAQSQVVTGPALITEEKTTIVVEPGWVALGTERGELLLEHRTSR
                     KSERVDLTACDPVLLEVFNGAFMNVAEQMGVVLQNTAQSVNIKERLDFSCALFDGEAN
                     LVANAPHMPVHLGSMDESVRVLLKAQRALMRAGDVYALNDPYHGGTHLPDVTVVMPIF
                     DDDGETLLFFVAARGHHADIGGITPGSMPPASRSIAEEGVLLDQVKIVEGGVFAEETL
                     LALLAGGSYPARDPRKNIADLRAQIAACEKGRRELLALTERYGFPCVRAYMQWVQDHA
                     EESVRRAISSLRDGEFSYSMDNGAVVRVRVSVDRAARSACVDFTGSGAQLESNFNAPR
                     AVVHAAVLYVFRTLVDADIPMNAGCLRPVRIVIPEGSMLAPRYPAATVAGNVETSQCV
                     TDALYGALGVMAAAQGTMNNFTFGNERYQYYETISGGSGAGPGFDGAAAVQTHMTNSR
                     MTDPEVIEQRFPVRVEEHSIRAGSGGKGRYRGGDGALRRIRFLQPMRAAILADRRIVA
                     PYGMAGGAPGEVGATWVERADGRRDALESCDEVDVQPDDVFAISTPSGGGYGTP"
     gene            complement(5771..6880)
                     /locus_tag="VFQ35_16670"
     CDS             complement(5771..6880)
                     /locus_tag="VFQ35_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9932341.1"
                     /translation="MIKHHPVYFALAAQLFACGAGQITDQSGGGALGGSTSSSAGGST
                     SDPASGGVATTNGGSGTTANGGSGTTGDGGSSTGDGGSSTTSNGGAATTGNGGQSSSA
                     NGGATGIGSGGTCATDALPAVVQSMLTNKCVACHGSVPITGVPSLVTYADLTRAAASD
                     AAKTNAEVALARIQNSASPMPPAPGASATAAEVAALSQFISAGYPKASCPTGGSGGSA
                     STGGAGAVDPLSAAPTCTSNTRWTQGNEGSASMNPGEACINCHKTTGGEGPSFSIAGT
                     VYPTGHEPDLCNSKVGTSGAQVVIVGADGQTVTLTPNSAGNFSLRTSIKTPYQAKVTF
                     QGRERLMLSAQTSGDCNSCHTQTGANGAPGRVTVP"
BASE COUNT         1200 a         2270 c         2287 g         1239 t
ORIGIN      
        1 cccgcgtgcg tagaaggtgc gctgcacctg tgtcccgccg aacgagcgat tgtcgagcat
       61 gccgccgtac tcgcgcgcga acggcacgcc ctgggcgacg cattgatcga tgatggccgt
      121 gctcacctcg gccaagcggt ggacgttggc ttcgcgggcc cggtagtcgc cgcccttgat
      181 ggtctcgtaa aacagccggt aaacgctgtc gccgtcgttc tggtaattct tggcggcgtt
      241 gatgccgccc tgcgcggcga tcgagtgggc gcggcgggga ctgtcttgga agcagaaggt
      301 ctttacgttg tacccgagct cacccagggt cgcggcggcc gaagcgcccg cgagccccgt
      361 gccgactacg atgacgtcca ggtgacgctt gttggcgggg ttgaccagct tgcaagtgga
      421 cttgtaggtc gtccacatct cggcgagcgg cgcgttcggg atgcgcgcgt cgaaggggcc
      481 tgtcggagcc gtgtctgttg ccatgttgga gcgctagttg agagagcgga ggtagaagta
      541 aatcggcatg atcgcgaagc cgagcgggac aatcaccgag aacgcggcgc cggcccggcg
      601 gatttgcagc gcgtagccgc gatggctgag gcccagcgtc tggaacgcgc tgcgaaaccc
      661 gtgcaacagg tggtaagcga gcgccaccat gcacacgacg tatacggccg agtacaagcc
      721 agaggagaac gactctcgaa ctgccgcgta catgttgcgc gtgccctccg catcgatcac
      781 ctggatgccg ccgctctccg gcgacgcgat gcgcgacttg tagaagaagt cggcgaggtg
      841 gatcaccaaa aagacggtga tgatgccgcc gagcgcgccc atgaagcgag acgaccaggt
      901 cgcgccgttg tcgcccttga gctcgtacgt cttcggccga gcggcgcggt tcttgaggac
      961 cagcgcgacg ccttctgcaa agtggaagat gatgcccgcg aagagcacgg cctccatgag
     1021 ccgcacgacg ccgttcgtgg ccatgaagtg cgcgtaggcg ttgaacgcgg cacccccgtc
     1081 gtccttgaac agaaggagat ttccgctcag gtgcacgacc agaaacgaaa tgaggaacaa
     1141 gcccgtgatc gccatcacga acttgcgacc aactgacgac gaaaccatgc cgatgtcctc
     1201 gaaccaagac ccgacgcgca tcaaaggcgc gtccgttgcg cgcggcgcaa tgttggcgcc
     1261 gtggggagtt tgtagggtct gccccacgat aagtccaatt caaataagtt cttgctaaga
     1321 taagtcagtc ttatgagcgg cgaggatggg cgctcgccgg cggctctgtc agactggccg
     1381 ccatgacggt ccgcctcttg atgctgaacg gctcgctccg cggaacctcc ggcaatacgg
     1441 aagagctcct ctcccttgcg gcgagctacg tcggcggcga cgtcacggtc gaacgcatcg
     1501 cgctcgcaga gtacgagggc agcgtcgaga atttggcggc gcggctcgcg gcgagcgacg
     1561 cctttctgtt cggcaccggg gtttactggg gctcatgggg ctccccgctg caacgctttc
     1621 tcgaggtgat gacgagctac gagctgacgc cgagcttttt gggcaagccg gcggcggtgg
     1681 tcgtcaacgc cgattctctc ggcgcgagcg acgttgccca ccggttgcag ggcgtactca
     1741 accatttcgg ttgctggagc ccgccgctcg ccagcgttgc cctcacgcgc gttgccgcgg
     1801 cagtgaaagg ccgcgctggc ttcgaagacg tgtttcaggt cgatgacatg cgcatcgctc
     1861 tcgagaacct gtgccgcgcc gcgcgcatcc cgtctccgca atggtcggct tggagtattg
     1921 cgcggacccc gagcgtctcc ggcgcttatc cgggggtcgg gccggtgctc gccggtttgc
     1981 ccaaatcgac gctgtgattt ctcagctcga gagcaccggg gcgctagggc gcgctaggga
     2041 gtgccgtatc cgccgcccga gggcgtcgaa atggcgaata cgtcatccgg ctgcacgtcg
     2101 acttcgtcgc atgactcgag cgcgtcgcgg cgcccatcgg ctcgctcgac ccatgtagcg
     2161 ccgacttctc ccggcgcgcc acccgccatg ccgtaaggag cgacgatgcg ccggtccgcc
     2221 aggatggcgg cgcgcatcgg ttgcagaaaa cggatgcgtc gcagcgcgcc atctcctccg
     2281 cggtaacgtc ctttcccgcc ggagccggcg cgaatcgagt gctcttcgac acgaacgggg
     2341 aaacgttgct cgatgacctc cggatcggtc atgcgggaat tggtcatgtg ggtttgcacc
     2401 gctgcggcac cgtcgaagcc ggggccggcg ccagagccgc ccgaaatggt ctcgtaatat
     2461 tggtagcgct cgttcccgaa agtgaagttg ttcatggtgc cttgagcagc ggccatgaca
     2521 ccgagcgcac catacagcgc gtccgtgaca cactgtgatg tttcgacgtt gcctgcgacg
     2581 gtcgcggccg ggtagcgcgg cgccagcatc gaaccttcgg ggatcacgat tcggaccggt
     2641 cgcaggcagc cggcgttcat cggaatgtcc gcgtccacca aagttcgaaa tacatagagc
     2701 acggcagcgt gcacgaccgc gcgcggggcg ttgaagttgc tctcgagctg ggcgcccgag
     2761 cccgtgaagt cgacacacgc agagcgcgcg gcgcgatcga cgctgacgcg caccctgacg
     2821 accgctccgt tgtccatcga atagctgaac tcgccgtcgc ggagagacga gatcgctctc
     2881 cgcacgctct cttctgcgtg gtcttgcacc cattgcatgt acgcgcgcac gcaggggaag
     2941 ccgtagcgct cggtcagcgc gagaagctct cggcgacctt tctcgcacgc ggcgatctgg
     3001 gcgcggaggt cggcgatgtt cttgcgtgga tcgcgcgctg ggtaggagcc gcccgcgagc
     3061 agcgcgagca gcgtctcttc tgcgaacacg ccgccttcga cgatcttgac ctgatcgagc
     3121 aggacgccct cctctgcgat actgcgcgag gccggtggca tcgagcccgg cgtgatgccg
     3181 ccgatgtcgg cgtggtggcc gcgcgccgcc acgaagaaca gcaacgtctc cccgtcatcg
     3241 tcgaagattg gcatcaccac cgtgacgtcg gggaggtggg ttcccccatg ataagggtcg
     3301 ttcaaagcgt agacgtcgcc ggcgcgcatg agagcgcgct gcgccttcag cagcacgcgc
     3361 acggactcgt ccatgctccc caagtgaacg ggcatgtgag gggcattcgc gaccaggttt
     3421 gcctcaccgt cgaacaacgc acacgagaag tctaggcgct ccttgatgtt taccgactgc
     3481 gcggtgttct gcagcacgac gcccatctgc tcggcgacgt tcatgaaggc gccgttgaac
     3541 acctcgagca acaccggatc gcaagcagtg agatcgaccc gctcggactt ccgcgaagtg
     3601 cggtgctcga gcaggagctc accgcgttcg gtgccgagcg cgacccagcc cggctcgacg
     3661 acaatggtcg tcttctcttc ggtgatcagc gcgggccccg tcacgacctg gctttgcgcc
     3721 aatgcggcgc tcggatacac cggcactcga gcccatcgac ccatgaccca cattggcgcc
     3781 gacccgctcg cttcgacttc gaatcggtta ctggtcgctg tgagcggcgg ttcgccttcg
     3841 cctcgcgcct cgaccgagat tgcttcggtg acgagttcac ggccgctcat cacgaacccg
     3901 aaccgcgctc gatgaagcct ttcgaactct agccgcattt ccaaaggctc cgcgaacgaa
     3961 acgacgagcg cggtgtccgt gccggcgtag cgaaggtgag cccgcgcgcg tgtttcgatc
     4021 gcctcgaggt cgacgctctg cgatgcgacc tctgcgctcg cggcttcgac gagctctgcc
     4081 acttcgtcaa cgagcctcgg ctgagcctct gcgctcaggg ttcgttccac ggctcgctct
     4141 tggatcgcgg tgatgcgcgc gagcccaacc ccgagcgccg aaaggaccga cgcgagtggg
     4201 tgaacgaaga tccttcgaat gccgagctct tcggcgacga ggcaggcatg ctgacctccc
     4261 gcgccgccga agcagcagag cacgtagtcg gcgagatcgt acccgcgctg cacggagatg
     4321 aacttgatcg cgttcgccat gttgttcacc gcgacccgca agaacccttc cgcgaccgct
     4381 tcgcgcgtct gcgattggcc cgtgcactgc gttatctctt cgcaaagcga ctcgaagccc
     4441 gcggtcactc cgtcgcgatc gagctcaccg ttcgcgtgcg ggccgaatac acgcgggaag
     4501 gcgctgggct ggattcggcc gagcaatacg ttacagtcgg tcaccgtgag cgggcctccg
     4561 cgccgataac aacgcggacc tggattggca cccgccgaat ccgggccgac ccgatagctg
     4621 tgtccgtcga aatgcagcac cgagccgccg cccgctgcga tggtgtgaat ggcgagcatc
     4681 ggcgcgcgca cgaacacccc ggcgatgcgc gtttcgaacg agcgctcgca gtcgctctcg
     4741 tctttgccgg aaaagtgcga gacgtcggtc gaggtgccgc ccatgtcgaa accaatgact
     4801 cggtcgaagc ctgcgccgag cgccgtccgg accattccga cgattccgcc cgccggtccc
     4861 gacaagattg cgtccttgcc ctgaaacgcg cgtgcttcgc tcagcccgcc gttcgacttc
     4921 atgaaataga ggggcacatc gccgagctcg ttcgccatcc gctcgacgta acgacggacg
     4981 accggcgaca ggtacgcgtc ggcgacggtc gtgtggccgc gtgagacaag cttcgagagc
     5041 gggctcaccc ggtgcgacgc cgagatttgc tcgaaccccg cctttcgcgc gagctcgagc
     5101 gcacggagct cgtgccgagg aaacgaatga gcgtgcatga atacgatcgc gcacgagcga
     5161 aaccctgccg agcgagcctc ggcgagtgcg agctcgagcg tgttctcgtc gagcggacgc
     5221 acgacggcgc cggtcgcgtc gagccgttcg tcgacttcga agacctgcgc atagagcggt
     5281 tcgggaagct cgatgtgccg cgcgaagagc cgcggtcggt tctggtaccc gatacggagc
     5341 tgatcgcgaa aaccgcgcgt gatgaagagc gccactggct cgccacgtcg ctcgagcaag
     5401 gcgttagtcg cgacggtcgt tcccattttc accgcctcga catgatcgac ggtgaggcgc
     5461 tctccggcgc cgagccccaa aagctgacgc atcccagcga gcgcggcgtc cgtgtactgt
     5521 tcggggttgt cggacaggag cttgatgctc cggcagctcc cgtctggacc gcgagcgacg
     5581 atgtccgtga aggtgccgcc tcgatcgatc caaaactggt agcgcgagtc gctcatgtgc
     5641 caacgccctc gtaggtcgtt gcatgtcgac gcgcaggtcg agcgtgaaca ggtcggagga
     5701 cgcgggcgag tgagcctgaa accatcggcg ccgctatgtt agccacagcg agcgcgcggt
     5761 tggtgcaaga tcagggcacg gtcacgcgcc ccggcgcgcc gttcgcgccg gtttgcgtgt
     5821 ggcaggagtt gcagtcgccg gaagtctgcg ccgacagcat caagcgttca cggccttgga
     5881 acgtgacttt ggcttggtac ggcgtcttga tcgacgttct cagcgagaag ttgcccgccg
     5941 agttcggcgt caaagtcacc gtctgtccgt ccgcgccgac gatcacgacc tgcgcgcccg
     6001 acgttcccac cttgctgttg cagaggtcag gttcgtggcc cgtcgggtaa acggtgcctg
     6061 cgatgctgaa agacgggcct tcgcctcccg tcgtcttgtg gcagttgatg cacgcttcgc
     6121 ccgggttcat cgaggcagag ccttcgttgc cctgagtcca gcgcgtgttg ctcgtacaag
     6181 tgggcgcggc ggaaagcggg tcgacggcgc cggcacctcc agtgctagct gacccaccgc
     6241 tgccgccggt tggacagctc gccttcggat acccggctga gatgaactgg ctcaacgccg
     6301 cgacctcggc ggctgtcgcg gaagcgccgg gcgcgggtgg catgggagag gcgctgtttt
     6361 gaatacgtgc gagcgccact tcggcgttcg tcttggccgc atcgctcgcg gcggcgcgcg
     6421 tcaaatcggc gtacgtcacc aaagaaggca ctcccgtgat gggaacggag ccgtgacacg
     6481 cgacgcactt gttcgtgagc atgctttgaa ccacggcggg cagtgcgtcg gtggcgcaag
     6541 tgccgccgga gccaatcccc gttgctccgc cgttcgcaga gctcgactgt cctccgttgc
     6601 cggttgtcgc agccccgcca ttgctcgtcg tcgaagaccc gccgtcgccc gtcgaagacc
     6661 cgccgtcgcc cgtcgtgcct gagcccccgt tggccgtcgt gcccgagcct ccgttcgtcg
     6721 ttgcaacacc tccacttgca ggatcggagg tgctgccgcc cgcgctcgac gacgtcgagc
     6781 cgcccagggc gccgcctccg ctctgatccg tgatttggcc tgcgccgcaa gcgaagagct
     6841 gggctgccaa cgcaaaatag acgggatggt gctttatcac gcgacgttac ctctgcgagc
     6901 agtgggtttt gcgagccacc aagcgcttgc gagaggtcag ttcccccatc cccgagaacc
     6961 actgcacgcc gaacccattt ttctcgtcag gggacc
//
LOCUS       DASTKS010000806         9775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1020479, whole genome shotgun sequence.
ACCESSION   DASTKS010000806 DASTKS010000000
VERSION     DASTKS010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9775
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020479"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFQ35_16675"
     CDS             complement(<1..629)
                     /locus_tag="VFQ35_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932342.1"
                     /translation="MLRRLVGVVGFLVLAGCSGADESESVAPSEALALLRSGGHGHDS
                     PVRAAIGSAAERLCDLAADRVGDPAHNGASDDDPDDGAWDWVLSPTALSHSPAASPEN
                     LYGSVALGPWAALRLGLAEMRNRQTLYNAFAGARANPNVDSPPDFVFLSLLGDVVTGA
                     APRLAKARYDAKVAQAGGARALASSIRDAHAASATDGLVAYDLAWLALAA"
     gene            842..1321
                     /locus_tag="VFQ35_16680"
     CDS             842..1321
                     /locus_tag="VFQ35_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9932343.1"
                     /translation="MTTDSTTVHALDRSKVAGVHQGGCHCGAVRFEVDFDPGKGATRC
                     NCSICVKTATTGVIVKPEAFRLLSGEDALSTYEWGHKVSRRYFCKHCGIHCFGRGHLD
                     VLGGDFVSLAVNCLDDVDLAELSILHWDGRHDNWYVGPRPEPWPIFTASNATPSGAT"
     gene            1497..2537
                     /locus_tag="VFQ35_16685"
     CDS             1497..2537
                     /locus_tag="VFQ35_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9932344.1"
                     /translation="MIAQRREQPLVLVALNEWKFPSEVAAKHIRAELRGLGASLVIVS
                     ASAVFCFRPDDEAHSAGLDDAGMRRLADSLCERYAVSPPAAGLTLVLVDADGRERWRT
                     NAPARADLCEALLEALSEAGRSVVEVGKTKDQLIPPDSLVRTRFSRRESVVLCLAAAL
                     AAALGESCKRTESRPRPQPSAAAKKASPTHEREIVLRVNGAERRVRVEPRVSLLDALR
                     ERMGLTGTKKGCDHGQCGACAVLVDGRRVNSCLTLAVMVENKAITTIEGLASGSQLHP
                     LQQSFASEDALQCGYCTPGQIMSALGLLHEGYATSDDDIREMMSGNICRCGAYANIVR
                     AVQNVRKTGLPA"
     gene            2534..3517
                     /locus_tag="VFQ35_16690"
     CDS             2534..3517
                     /locus_tag="VFQ35_16690"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9932345.1"
                     /translation="MKDFDYVVAKSAEEAARQGKAANARYIAGGTLLVDLMRLNVERP
                     ELVIDVNQALSSEIRAEDDRLFIGALARNSDLAHHERVKSDYPVLSEALLAGASPQLR
                     NMATVGGNLLQRTRCAYFRDPAFRECNKRHPGSGCAAMQGFTRMHAILGGSDHCIAAH
                     PSDMSVALLALDAVIHTQGDGTSRAIPIAEFHTLPGDHPEIETALTKGELVTSVEVPR
                     QGFSKTSAYVKARDRASYAFALASAGVALEFDGGRIRAARVALGGVSTKPWRAAETEA
                     ALVGSIPSEDSFASAAKLAANGAKTTPDNDFKRKLTERVVLAALTRAWKRS"
     gene            3514..5727
                     /locus_tag="VFQ35_16695"
     CDS             3514..5727
                     /locus_tag="VFQ35_16695"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9932346.1"
                     /translation="MNKPLIGKPVDRVDAKGKVTGAIEYAADVPVANVAHAVIVTSGV
                     GRGRVETVDVEAARHMAGVLAVITPENAPKLPGASARKDPNDRVLQLLQDAEISFNDH
                     PIAVVVADTLERAREAADTLAVKYSSGSGVQVHLAEHASDVYTPRTAGPREKPDTSRG
                     DIEQALSSAAVKIDQTYRTPSQNHNPMEMHGLTAVWQGKDALTVYDTSQGIFTVKKKL
                     AAVFGISPTDVRVISHYVGGGFGCKGTPWSHVALCALAARVVNRPVKLVLTRQQMFSL
                     VGHRPETIQHVVLGADRSGKLTSMKHEVISSTSRFDEFSEPSALQTRMLYACPSVATS
                     HRLVRLDIPTPTFMRAPGEATGTFALEVAMDELATELGIDPVELRLRNYAERDPHEDK
                     PWSSKSLRECYKRAAETFGWARRKPAPRSMRDGRMLVGWGMATATYPARLTPASARVT
                     LKKDGSIVVQAGSQDLGTGTYTILSQIAADVLGVPLERVRTEIGDTALPETPVSGGSQ
                     TAASTGSAVKLAAEELKKKLVQTALADSQSPLFSANADHVDVSDGALLLRGSSKREDY
                     QALVARAPANELSAEVKSNEKPERKSRSCHSFGAQFVEVKVDPDTCEIRVTRCVSAFA
                     AGKILNEKTARSQYMGGMVWGIGFALEEHTVRDGRNGRVVTRDLADYHVPVNADVPAL
                     TVIMVDEEDPYVSETGSKGIGEIGITGISAAIANAVYHATGKRIRDLPITLDKLL"
     gene            5735..6883
                     /locus_tag="VFQ35_16700"
     CDS             5735..6883
                     /locus_tag="VFQ35_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9932347.1"
                     /translation="MNETEMICRAAALVKERGEASWLASVVRVRGSAYRRAGARLLFT
                     REAAVAGSISAGCIENALVQSGSFFAADRPVLRTFEAALPDDDEPQPGSGCGGSVDVL
                     IEPVVEGCADTLAFIGEALAGQQRVAMVTVVHSSSRFGGLGARVVRSGNQSLSVGCEL
                     ALESELELFARESLETGPAKARCIRRGALELLLEVLEPSPRLFVFGAGPDVPPLVSLA
                     SQLGWDVTVVSRRDRPALRERFVGLPCRYVVSDAAALVPAINRSTRALAVVMSHDFAN
                     DSDCLEALLQANPHYLGVLGPLARTQRLLSEIENRGGDVLGGLSRLHAPVGLNIGAET
                     SAEIALAIVAEAQARLKGADAQTLREKSGPVHAPPLPDLRLALAEAAE"
     gene            6880..7443
                     /locus_tag="VFQ35_16705"
     CDS             6880..7443
                     /locus_tag="VFQ35_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9932348.1"
                     /translation="MIGGAVLAAGASRRLGSPKQLLRVHGTTFVRHAALCTLSAADRS
                     AVVVGHGAEAVIASLGELPLETLENAEWSEGIASSIRLATGWARDNAFEALLLVLTDQ
                     PGLSAVHLQRLCARYRVTGAPVASRYSGVLGAPAIFPASYFAALDRSSGDRGAGALLR
                     QTIDAEAIDCPALELDVDTEAAARAFR"
     gene            complement(7505..>9775)
                     /locus_tag="VFQ35_16710"
     CDS             complement(7505..>9775)
                     /locus_tag="VFQ35_16710"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HET9932349.1"
                     /translation="NSQRHRLTPWSNDATRDPSGEVLYLRDETDGTSWSLTPAPAGRE
                     AEFLVRHGAGYTHFEHTRGELEHLLTVFVSAADPVKFFRVRLANRGARARKLSLFGVV
                     EWVLGGVRDSAHLTTATCWDAERRAALAFNPWSVFTEHRAVFFSTNPVTSFTCDRKEW
                     FGRFGSRERPSALERAGLSGRTGVGLDPCAALQVSLELAPGDEVELAFALADGESVDH
                     ARTLAERYGDMKAVNAAFDQMNEHWEKTLGAVQVKTPDSAFDVMANRWLLYQALSCRI
                     WARTAFYQSSGAYGYRDQVQDVLALLLSKPELAREHLLRAAARQFAEGDVQHWWHPET
                     GEGIRSRCSDDLLWLPYATAQYVLATADKSILEERVPVLEERQLAPQESDLFGSPPAS
                     KEGVPLYEHCVRSLMRGATEGPHGLPLMRGGDWNDGMNRVGREGKGESVWLSWFLAKT
                     LKDFALVAKQQGDAVRATWCTQQVTRLAEAIDASAWDGAWYRRAIFDDGSPLGSAQNT
                     ECQIDAIAQSWAVISGVGDERRAAQALQSAEQRLILEDERMMLLLTPPFERAEPDPGY
                     IRSYPPGIRENGGQYTHGVLWTVVALCLLGEGDRAYRLMSYLNPVHHGENLEQLARYR
                     VEPYVVAADVYSSAEHQGRGGWTWYTGSASWMYRDMVESILGLRKENGRLRIAPCLPR
                     DWPGFEATYRYGNSELEIVVENPERISSGTVQLSVDDKPEPTGAVALVDDGRRHRVRA
                     VLRKPLEEARRRVSGS"
BASE COUNT         1720 a         3262 c         3192 g         1601 t
ORIGIN      
        1 gcggcgaggg cgagccaggc gagatcgtaa gcgacgaggc cgtccgtcgc ggaggcggca
       61 tgcgcgtcac gaatgctcga cgcgagggcg cgcgcaccac cggcttgcgc gaccttcgcg
      121 tcgtagcgcg ctttcgcgag ccgcggggca gcgcctgtga cgacgtctcc tagcaagctc
      181 aagaagacga aatccggcgg cgagtcgacg ttcgggttcg cgcgcgcacc ggcgaaggca
      241 ttgtaaaggg tttggcggtt ccgcatctcc gctagcccta accgcagcgc cgcccacggc
      301 ccgagcgcga ccgaaccgta aaggttctcc gggctcgccg ccggcgagtg gctcagcgcg
      361 gtcgggctca gcacccaatc ccatgcgccg tcgtcgggat cgtcgtcgct cgctccgttg
      421 tgagccggat cacccactcg atccgcggcc aagtcgcaca agcgctcggc cgcgctcccg
      481 atcgccgcgc ggacgggaga atcgtgacca tgcccaccgc tccgaaggag ggccaaggcc
      541 tcgctcggcg cgaccgactc ggattcgtcc gcccccgaac accctgccag caccaaaaag
      601 ccaacgactc cgactagacg acgaagcatg accgcctcct ccactctggt tgcgacgtga
      661 gtcgccagtt tgccaacttc tcgcgtcttg gttggtgtcg cgcgagacgt ccgacgttca
      721 agtggcggaa gccgctccta agcttttgct ttcgctggtg aatttcgagc ctgcgaaaca
      781 ctgacaatac gctgtcattc ggatgggcga tggtgggctt cgttgctcac ggaggttcac
      841 gatgacaact gattccacga cggttcacgc gctcgatcga agcaaggttg caggggttca
      901 ccagggagga tgtcactgcg gggcagttcg cttcgaagtc gacttcgacc caggcaaggg
      961 cgccacccgc tgcaattgca gcatctgcgt gaagaccgct accaccggcg tgatcgtgaa
     1021 gcccgaagcc ttcagacttc tttcaggcga ggacgctctg tccacgtacg agtggggcca
     1081 caaggtgtca cgccgatatt tctgcaagca ctgcggcatc cactgcttcg ggcgcggtca
     1141 cttggacgta ctcggcgggg acttcgtctc cctcgccgtg aactgcctcg acgacgtcga
     1201 cctcgccgag ttgagcatcc ttcactggga tgggcgccac gacaactggt acgtggggcc
     1261 gcgtcccgag ccgtggccca tcttcacggc gagcaacgcg acgccgagcg gcgcaacctg
     1321 aagagtcgct cagcgctgcc ccagcgtggc gctgctgcgc tcggggcagc gccgcatctg
     1381 catgcaaacg gagcctttcg cgcgctcgct ggatgcacgg tgggaacgaa acccgaggta
     1441 cgaaaattga atcagtcgcg cggatgtcga gcgccggatc cgcgacagca ctggacgtga
     1501 tcgcgcagag gcgcgaacaa cccttggtgc tcgttgcgct caacgagtgg aagttcccga
     1561 gcgaggtggc ggcgaagcac atccgcgcgg agctccgcgg gcttggggcg tcgctggtca
     1621 tcgtgtcggc gagcgctgtc ttctgcttcc gtcccgacga cgaagcgcat agcgccgggc
     1681 tggacgacgc tggtatgcgc cgcctcgccg actcgctctg cgagcggtac gcggtttcgc
     1741 cgcccgcggc tggtctcact ctggttttgg tcgacgccga cggacgcgag cgttggcgca
     1801 cgaacgctcc cgctcgtgca gatctctgtg aggccctatt ggaagcgctg agcgaggcgg
     1861 gccgcagcgt cgtcgaagtg ggcaagacca aagatcagct gatcccgccc gacagtttgg
     1921 tgcgcacgcg cttcagccgc cgcgaaagcg tggtgttgtg cctggcagcc gcgctcgcgg
     1981 ccgcgctcgg tgaaagctgc aagcgcaccg agtcgcgccc tcgaccgcaa ccctcagccg
     2041 ccgccaagaa ggcgtctccg actcacgagc gcgagatcgt tctgcgcgtg aacggcgccg
     2101 agcgccgcgt gcgtgtcgag ccacgcgtgt cactgctcga tgcgctccgc gagcgcatgg
     2161 gtcttacggg tacgaagaag ggctgcgacc acggtcagtg cggcgcctgc gccgtgctcg
     2221 tcgacggtcg ccgcgtgaac tcctgcctga cgctcgcagt gatggtcgag aacaaggcga
     2281 tcacgacgat cgagggactg gcgagtggct ctcagctgca cccgctccag cagtcgttcg
     2341 cgagcgaaga cgctctgcag tgcggctact gcactccggg ccaaatcatg agcgcgctcg
     2401 ggctgctgca cgagggatac gcgaccagcg acgacgacat tcgagagatg atgagcggaa
     2461 acatctgccg ctgtggcgcc tacgccaaca tcgttcgcgc ggtccaaaac gtgcgcaaga
     2521 cgggactacc ggcatgaaag acttcgatta cgtggtcgcg aagtcggcgg aggaggccgc
     2581 tcggcaggga aaagctgcga acgcgcgcta catcgcgggg ggcactttgc tcgtcgatct
     2641 gatgcgcctc aacgtcgagc gaccggagct cgtcatcgac gtcaaccaag ccctctcgag
     2701 cgagatccgc gcggaggacg accgcttgtt catcggcgcg ctcgcgcgca acagcgatct
     2761 cgcccaccac gagcgcgtga agagcgacta cccggtgcta tcggaggcgc tcctcgcggg
     2821 cgcttcaccg cagctccgca acatggcgac ggtgggcggc aacttgctgc agcgcacgcg
     2881 ttgcgcgtac tttcgtgacc ctgccttccg cgagtgcaac aagcgtcacc ctggtagcgg
     2941 ctgcgcggca atgcagggat tcacgcgcat gcacgcgatt ctcggcggaa gtgaccattg
     3001 tatcgccgcg cacccttccg acatgtcggt tgcgttgctc gcgctcgacg ccgtgattca
     3061 cacccagggg gacggcacga gccgcgcgat ccccatcgcc gagttccaca cgctgccagg
     3121 cgaccacccc gagatcgaga cggcgctcac caagggagag ctcgtgacga gcgtcgaagt
     3181 tccgcgccaa ggcttctcga agacgtcggc ttacgtcaag gctcgcgata gagcatctta
     3241 tgcttttgcg ctcgcttcgg cgggcgtagc tctcgaattc gatggcggcc gcatccgggc
     3301 cgcacgcgtc gccttaggag gagtctcgac caagccctgg cgggcggcgg agaccgaagc
     3361 cgcgctcgtc ggcagcatcc cgagcgagga ctccttcgcg agcgcggcca agttggccgc
     3421 gaacggcgcg aagacgacgc ccgacaacga cttcaaacgc aagctgaccg agcgcgtcgt
     3481 gctcgcggcg ctcactcgag cgtggaaaag gtcatgaaca agccattgat cggtaagcca
     3541 gtcgaccgcg tcgacgcgaa gggcaaggtg acgggcgcca tcgagtacgc cgcagacgtc
     3601 ccggtcgcga acgtggcgca cgccgtgatc gtcacgagcg gagtaggtag aggccgcgta
     3661 gagaccgtcg atgtcgaagc cgcgcgccac atggcgggcg tgctcgccgt gataacgccc
     3721 gagaacgcgc cgaagcttcc cggcgcgagc gcgcgaaagg atccgaacga tcgcgtgctc
     3781 cagctgctgc aagacgccga gatctctttc aatgatcatc cgatcgcggt cgtggtagcc
     3841 gacactctgg agcgcgcgcg cgaggcggcc gatactctcg ccgtgaagta ctcgagtggc
     3901 agcggtgtcc aggtacacct cgccgagcat gcatccgatg tgtacacgcc gaggacggcc
     3961 ggtccgcggg agaagccgga cacgtcgcgc ggcgacatcg agcaggcgct ctcgagcgcg
     4021 gccgtgaaga tcgaccagac gtaccggacg ccctcccaga accacaaccc gatggagatg
     4081 cacggcctca ccgcggtttg gcaaggcaaa gacgcgctca ccgtttacga tacctcccag
     4141 ggcatcttta ccgtcaaaaa gaagctcgcg gcggtgtttg gaatctcgcc gaccgacgtg
     4201 cgcgtgatat ctcactacgt gggcggcggc ttcgggtgca agggcacgcc gtggtcgcac
     4261 gtggcgctgt gcgcgctcgc ggctcgggtg gtgaaccgcc cggtcaagct ggtgctcacg
     4321 cgtcagcaga tgttttctct ggtcgggcac cgacccgaaa cgatccagca cgtggtgctc
     4381 ggcgcggacc gcagcggcaa gctgacgtcg atgaagcacg aggtcatctc ctcaacctcg
     4441 cgcttcgacg agttctcgga gccgagcgcg ctccaaaccc gcatgctgta cgcatgcccg
     4501 agcgttgcca cgagccaccg gctagtgcgc ctcgacattc cgactcccac cttcatgcgt
     4561 gctccgggtg aggctacggg cacgttcgcg ctcgaagtgg cgatggacga gctcgcgaca
     4621 gagctcggca tcgatccggt cgagcttcgc ttgcgcaact acgcagagcg cgacccgcac
     4681 gaagacaagc catggtcgag caagtcgctg cgagaatgct acaagcgcgc agccgagacc
     4741 ttcggttggg cgcgccgcaa accagcgccg cgcagcatgc gcgacgggcg gatgctcgtc
     4801 ggctggggca tggctaccgc cacttatcca gcgcgtctca cccccgcttc ggcccgagtc
     4861 accttgaaga aggacgggag catcgtcgta caagcgggca gtcaagatct gggcaccggc
     4921 acgtacacga tcctgtcgca gatcgcggca gacgtactcg gcgtaccgct cgagcgggtc
     4981 cgcaccgaga ttggggacac ggcgctgccg gagacgcccg tctctggcgg ctcgcaaacc
     5041 gcggcgagca ctggctcggc ggtgaaactg gcggcggagg agctcaagaa gaagctcgtt
     5101 caaaccgcgc tcgccgatag ccaatcgccg ctcttttccg ccaacgccga ccacgtcgac
     5161 gtgagtgatg gcgccttgct cctgcgcggt tcgagcaaac gcgaggacta tcaggcgctc
     5221 gtggcgcgcg cgccggcaaa cgagctcagc gccgaggtga agagcaacga aaagccggag
     5281 cgcaagtcgc gctcttgtca ctccttcgga gctcagttcg tcgaggtcaa ggtcgatccg
     5341 gacacttgcg aaatccgagt cacgcgctgc gtatcggcgt tcgccgcagg caagattctg
     5401 aacgagaaga cggcgcgcag tcagtacatg ggcggcatgg tttgggggat cgggttcgcg
     5461 ctcgaggagc atacggtgcg cgacggccgc aacgggcgcg tcgttacgcg agacctcgcg
     5521 gactatcacg ttcccgtgaa cgccgacgtt ccagctctca ccgtcatcat ggtcgacgaa
     5581 gaagatccct acgtgagcga gacgggcagc aaagggatcg gcgaaattgg aataaccggg
     5641 atctcggccg ccatcgcgaa cgccgtctac catgccaccg ggaagcgaat tcgggacctt
     5701 cccatcacgc tcgacaaact tttgtgaggg cgacgtgaac gaaaccgaga tgatttgcag
     5761 agcggccgcg ctcgtaaagg aacgaggcga agcgagctgg ctcgcgagcg tagttcgcgt
     5821 acgcggatcg gcctatcggc gcgcgggcgc gcggctcctg ttcacgcgcg aggcggctgt
     5881 ggctggctcg atcagtgccg ggtgcatcga aaacgcgctc gtgcaaagcg gctcgttctt
     5941 cgccgccgat aggccggtgc tgcgcacctt cgaagccgcc ctgcccgacg acgacgaacc
     6001 ccagcccggc agcggctgcg gcggctcggt cgacgtcctg atcgagccgg tcgtagaggg
     6061 ctgcgccgac acgctcgcat tcatcggtga ggcgctcgcc gggcagcagc gcgtcgcgat
     6121 ggtcacggtt gtgcacagca gctctcgctt cggcgggctc ggcgcgcggg tcgtccgatc
     6181 cggcaaccag agcctgagcg tcggttgcga gctcgccctc gaaagcgagc tcgagctctt
     6241 cgcgcgcgag agcctcgaga ccggccccgc gaaggctcgg tgcatccgcc gcggggcgct
     6301 cgagctgttg ctcgaggtgc tcgaaccttc ccctcgcctc ttcgttttcg gggccggccc
     6361 cgacgtcccg ccgctcgtct cgctggcgag tcagctcggt tgggacgtca cggtggtgag
     6421 ccgccgtgac cgcccggccc tgcgcgagcg cttcgtcggg ctcccgtgtc gctacgtcgt
     6481 gagtgatgcc gccgcgctcg ttcccgccat caatcgctcg acgcgagccc tcgccgtcgt
     6541 catgtcgcac gacttcgcga acgactccga ctgcttggag gccctactgc aagcgaaccc
     6601 ccactacctt ggcgtgctgg ggccgctcgc tcgcacgcag cgactacttt cggagatcga
     6661 gaatcgcggc ggtgacgtgc tcggcgggct gagccgcctg cacgcgcccg tgggattgaa
     6721 catcggcgcc gaaacctcgg ctgaaatcgc gctcgcgatc gtggccgaag cgcaagctcg
     6781 tttgaagggc gccgatgccc aaaccctgcg cgaaaagagc ggccccgtgc acgcccctcc
     6841 cctccccgat ttgcggctag cgctcgccga ggcagccgaa tgataggagg cgccgtactc
     6901 gcggcaggag catcgcggcg cctgggctct cccaaacaac tgctgcgagt tcatggcacg
     6961 accttcgtcc gacacgctgc gctgtgcacg ctgagcgcgg cggaccgctc agcggtggtc
     7021 gtcgggcacg gcgcggaggc agtgatagcg agcctcgggg agctgccttt ggagacactc
     7081 gagaacgccg agtggagcga gggcatcgcg tcttcgatac ggctcgccac cggttgggcg
     7141 cgagacaacg ccttcgaagc tttgctgctc gttctgaccg accagccagg gctcagcgcc
     7201 gttcacctcc aacgtttgtg cgcccgctac cgcgttacgg gcgcgccggt cgcttccagg
     7261 tactccggcg tcctcggcgc gcccgccatc ttccccgcga gctactttgc ggccctcgat
     7321 cgctcgagcg gtgatcgcgg cgccggcgcg ctacttcgcc aaaccatcga cgctgaagcc
     7381 atcgactgcc cggcgctcga gctcgacgtc gacaccgaag cggcggcgcg agcctttcgc
     7441 tgagcgaatc ggcgaatgcc tgcgttggcg atcgcatgca gcagacgccg cgacgtgtgc
     7501 tcgctcaact tccggagacg cgacggcgcg cttcctcgag gggctttcgc aagacggctc
     7561 ggacccggtg acgtcgccca tcgtcgacca acgcgaccgc tcccgtaggt tcgggcttgt
     7621 cgtccacgct gagctgcacg gtcccgctcg aaatgcgctc cggattctcc acgacgattt
     7681 cgagctccga gtttccgtat cggtacgttg cctcgaagcc gggccagtcg cgcggcagac
     7741 agggcgcaat gcgcagtcgg ccgttttcct tccgcaagcc gaggatgctc tcgaccatgt
     7801 cgcggtacat ccacgaagcc gagccggtat accaggtcca gccgccgcgg ccttggtgct
     7861 ctgcagacga atacacatcg gcggcaacga catacggctc gactcgatag cgcgcgagct
     7921 gctccaggtt ctcgccgtgg tgcaccggat tcaagtagct catcaggcgg tacgcgcggt
     7981 cgccctcacc gagcaggcaa agcgcaacta ccgtccacaa gacgccgtgc gtgtactgtc
     8041 cgccgttttc tcggatgccc ggtggatagg aacgaatgta ccccgggtcg ggctcagcgc
     8101 gctcgaaagg cggggtgagc aagagcatca tccgttcgtc ttcgaggatg aggcgctgct
     8161 cggcgctctg tagagcttga gccgcccggc gctcgtcgcc aacgccggat attacggccc
     8221 aagactgcgc aatcgcgtcg atttgacatt cggtgttttg agccgagccg agcggagaac
     8281 catcgtcgaa aattgcgcgc cggtaccacg ctccgtccca agcgctcgcg tcgatcgcct
     8341 cggcgagccg cgtaacctgt tgagtgcacc aagtggcacg taccgcgtca ccctgctgct
     8401 tggcgaccaa ggcgaagtcc ttcaacgtct tcgcgaggaa ccacgagagc cagacgctct
     8461 cgcccttgcc ctcgcgccca acccggttca tgccgtcgtt ccagtctccg ccgcgcatca
     8521 acggcaagcc gtgcggccct tcggtggcgc cgcgcatcag cgagcgcacg caatgttcgt
     8581 agagcggaac gccttctttc gacgccggag gggaaccgaa caagtcgctt tcctggggcg
     8641 cgagctggcg ctcttcgagc acgggcacgc gctcttcgag aatgctcttg tctgccgtcg
     8701 cgaggacgta ctgcgccgtt gcgtagggca gccacaaaag gtcgtccgag cagcgcgagc
     8761 ggatgccctc acccgtctcg gggtgccacc aatgttgaac gtcaccctcg gcaaattgcc
     8821 gcgccgccgc gcgcagcagg tgctcccgcg cgagctccgg cttgctcaac aacaaggcga
     8881 gtacgtcttg cacttgatcg cggtaaccgt aagcgccgct cgattggtag aaagcagtgc
     8941 gcgcccagat gcgacaggac agcgcctgat acagaagcca gcggttcgcc atcacgtcga
     9001 aggcagaatc cggggtcttc acctgaaccg cgccgagcgt cttttcccag tgctcgttca
     9061 tctggtcgaa ggcggcgttc acagccttca tgtcgccgta acgttcggcg agggtgcgag
     9121 cgtggtcaac gctctcgccg tcggcgagcg caaaggcgag ctcgacttcg tcgcccggcg
     9181 caagctcgag cgatacctgc aaggcggcgc acggatccag accaacgcca gtccgcccgg
     9241 agaggcccgc ccgctccagc gccgacggac gctcgcgcga gccgaagcga ccgaaccact
     9301 ccttgcggtc gcacgtaaag ctggtcacgg ggttggtcga gaagaacacc gcgcgatgct
     9361 cggtgaacac cgaccacggg ttgaaggcca aagccgcacg acgctccgcg tcccagcacg
     9421 tcgcggtagt gaggtgcgcg ctgtcgcgaa ctcccccgag cacccactcg acgacgccga
     9481 acaacgagag ctttcgtgcg cgggctccac ggttcgcgag cctcacacgg aaaaacttca
     9541 ccgggtcggc cgcgctgacg aacaccgtga ggaggtgttc gagctctccc cgggtatgct
     9601 cgaagtgcgt gtagcccgcg ccgtgccgca cgaggaactc cgcttctctg cctgcaggcg
     9661 cgggagtcaa cgaccaactc gttccgtcgg tctcgtcgcg taggtagagc acctcgccgc
     9721 tcggatcgcg agttgcgtcg ttgctccacg gggtgaggcg atgccgctgg ctgtt
//
LOCUS       DASTKS010000807         5223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_129252, whole genome shotgun sequence.
ACCESSION   DASTKS010000807 DASTKS010000000
VERSION     DASTKS010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129252"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFQ35_16715"
     CDS             <1..213
                     /locus_tag="VFQ35_16715"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET9932350.1"
                     /translation="TRLLLPVLRARTLLCLRKDRQQFEQELDRVLRENDPNPSLRLEN
                     AAAKRRARRYLTSRVVDAECFAPSAP"
     gene            complement(239..1678)
                     /locus_tag="VFQ35_16720"
     CDS             complement(239..1678)
                     /locus_tag="VFQ35_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9932351.1"
                     /translation="MAAPIAASGVNVSTSGSPYRVVLRLATPTILAMLAQSAVNEIDI
                     IFFARLPCPESSNAQAALLPSLILLWMFGGSLSAISVGTQAIAARRFAERRPTDAGGV
                     LLNSWLFSLVSGIVFTLLGYLAMPFVLSALIKVPEVREAARGYLGWRLLGITSMAMTF
                     SFKAFFDGIGKTYVHMVSSIVMNIINVVLCVMFIFGMWGAPRMGMAGAGFAGFVSTWV
                     GLAIMIAWAMSPQLARRFEPFALGRWSRRITSDILRLSIPSAVATVAVMSGFALFSMI
                     VSHLDSTSHAEVVSASCPGGHAEAVNSAATMLIVGILKLTFTACLAFGTSTATLVSQS
                     LGEGDPDRATRFGWVSVRLGLVIFGVVGLLEGVVFTRPLLSVVTHSPAVLEAALGPMR
                     LMGICTPLIATGMILTQALFGAGNTRFVMLVELVLHFLCLVPLAWLLGITMGFGLKGI
                     WASATTYVVLLTAVMVWKFKTGDWRDIRL"
     gene            complement(1851..4694)
                     /locus_tag="VFQ35_16725"
     CDS             complement(1851..4694)
                     /locus_tag="VFQ35_16725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932352.1"
                     /translation="MSPLELTDNSLKSQEINPGSQAPQEVDLPLPRRFERFTLLARVA
                     RGGMGEVYLAAARGIEGAERPLIVKIIRPDHAQDRSFVARFLDEARIQAQLQHPGVAQ
                     ILEATTDAEGKPYVVVEYVEGRNLGDVRTRSGQLGLRISWAEAVAIGTSLGDALAHVH
                     ERTDAEGRPLEIVHRDLSPQNVMVGYGGELKIIDFGTARGQNRRCQTISGIVFAKPGY
                     VAPEVANNQPGGIPADLYAFGVMLWELLAGRRFLTGEATQHLTEVGAGRRPLVPIAKL
                     IGAPAELDLILARLTAQRIEDRYESARKATQDLVKLLQRATSLADGERSVRARVADLM
                     RRLYPAEPARSRGEFVKRLAQARSLEPKHPLPEASPEAPEATEPGMLSGTRYRLVREI
                     GRGAMGIVHQAVHVDLSRTVALKLLSAETMSAAAQSRFRAEARAIAQLSHENLVKVFD
                     FGMCRDGRLFYAMELLDGQTLAERLDREQRLTAASVIDFGIQACRALDAAHRAKVIHR
                     DIKPANLFVTRDGTLKVLDFGLATSITAADAPPNAENPVALVGTPEYMAPEQARGESD
                     ARSDVYALGAVLYELCTGFLPHDADAPLAIIAEKQKRPERPSRRVEGLQLPRALENAI
                     LRALSPSPEDRPESAEQLAKELEAALHEPRRRSVIRRRLGAVAVGVTLVGCFAVLGSA
                     FARSPRLSGLVREHASEGLQTVTGAWDRLAERRAALVSARQAPPGASPKVNDVRALPP
                     IEATSVHEANAVQVEEANSKAPEVTPTVEATPKTNAEASEQKVAGLDADAETTDPSAD
                     DKAAEEADESSGSSDASSQGIKGAAASKVTEQLERARVLAEKGRELRALALLRSLARR
                     HAKDPNVLAALTQALQKNRSWGEALKVARQRVSVDGSPQARLELARVERASGHRERAI
                     ALLNPLTQVAETQAAARELLRSLGGTERVALRQ"
     gene            complement(4731..>5223)
                     /locus_tag="VFQ35_16730"
     CDS             complement(4731..>5223)
                     /locus_tag="VFQ35_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932353.1"
                     /translation="FLGGRSNFRAYPASSPLELASSGTRARGTGPKRYTMGGRRGAAS
                     RLVLGGEGALLGAESTALGAVFRSQLSGTSFAARLIGGAAIVSACSPHMGTLVNADAR
                     DETQNVSAMIQTHKPMRRAKSATNQLVKDQCIMGRARQSNPVAAQLNENPAAGIPELS
                     TDE"
BASE COUNT          945 a         1742 c         1666 g          870 t
ORIGIN      
        1 acgcggctac tattacctgt gctgcgcgcc agaacgctgc tgtgcctgcg taaagatcgt
       61 cagcagttcg agcaagagct cgaccgggtg ctgcgcgaaa atgacccgaa ccccagtctg
      121 cgactggaaa atgcggcggc caagcggcgt gcacggcgct atctgacgag ccgtgtcgtc
      181 gacgcggagt gcttcgctcc gtccgcgccc tgagccgagc cgcgctgggt taacccgact
      241 agagccgaat atcgcgccag tcaccggtct tgaatttcca gaccatgacc gcggtgagca
      301 gcacgacgta ggtcgtggcc gaagcccaaa ttcccttcag gccgaagccc atcgtgatgc
      361 ccagcaacca ggcgagcggc accagacaca agaagtgcag caccagctcc acgagcatca
      421 cgaagcgggt gttgcccgcg ccaaacaagg cttgcgtcag gatcatgccc gtcgcgatca
      481 gcggcgtaca gatccccatc aagcgcatcg gacccaaggc ggcctcgagc acggccgggc
      541 tatgcgtcac gacggacaac agcggccgcg tgaacacgac gccttcgagc aagcccacga
      601 cgccgaagat taccaggccg agcctcacgg aaacccagcc aaagcgagtc gcccggtcgg
      661 gatccccctc cccgagcgat tgactgacga gggtcgcggt agaggtcccg aacgccaagc
      721 acgccgtaaa cgttagcttc aagatgccca cgatcagcat cgtcgcggcg ctgttgaccg
      781 cttcggcgtg cccacctggg cagctcgcgc tgacgacctc ggcgtgtgac gtcgagtcca
      841 gatggctcac gatcatggag aacagagcga agccgctcat caccgctacg gtggccaccg
      901 cgctcggaat cgacaggcgc aagatgtcgc tcgtaatccg cctcgaccag cgcccgagcg
      961 cgaagggctc gaagcgacgc gccagctgcg gagacatggc ccatgcgatc atgatcgcca
     1021 agccgaccca ggtggatacg aaacccgcga agcctgcgcc ggccatgccc atgcgcggcg
     1081 cgccccacat cccgaagatg aacatcacgc acagcacgac gttgatgatg ttcatcacga
     1141 tcgacgacac catgtgcacg tacgtcttac cgatgccgtc gaagaaggct ttgaacgaga
     1201 acgtcatcgc catcgacgtg atcccgagca aacgccagcc caagtaccct cgcgcggcct
     1261 cacggacctc cgggaccttg atcaacgccg agagcacgaa cggcatcgcc aagtagccga
     1321 gcaacgtgaa gacgatgccg gagaccagcg agaacaacca ggagttgagc agtaccccgc
     1381 ccgcgtccgt gggccgtcgc tccgcgaagc ggcgcgccgc gatggcctgc gtgcccaccg
     1441 aaatcgcgct caaggagccg ccgaacatcc agagcaaaat caacgagggc agcagcgccg
     1501 cctgagcatt cgaggactca gggcacggca gccgcgcaaa gaagatgatg tcgatctcgt
     1561 tgaccgcgct ctgcgcgagc atcgcgagga tcgtcggcgt ggcgagtcgc aggacgacac
     1621 ggtacgggga cccgctcgtt gatacgttca cgccagacgc ggcaatgggc gctgccataa
     1681 gggtgcttgg gggtggcgga ctcgcgtttc tatgagcatt ccggcctttc ggcaagcgcc
     1741 gaaggccgga agtcagcgat ttccaccggc cctaggggcc agcacctgat tctcgcaggg
     1801 ttacagtagc ttcacgcaag cgtcatttgg ctcgcgggag caacacgccg tcattggcgc
     1861 agagcaacgc gctcggtgcc gccgagcgag cggagtagct cgcgagcagc cgcctgcgtt
     1921 tctgccacct gggtgagcgg gttcagcagt gcgatcgctc gttcgcggtg tccggatgcc
     1981 cgctcgacac gcgccagttc gaggcgtgct tgcgggctgc cgtcgacgct gacgcgctga
     2041 cgggcaacct tcagcgcctc tccccaggaa cggttcttct gtagggcttg agtcagcgcc
     2101 gcgagtacgt tcgggtcctt cgcgtgacga cgggccaagc ttcgcagcag ggccagtgcg
     2161 cgaagctcgc ggcctttttc ggctagaact cgagcacgct cgagctgctc ggtaactttg
     2221 ctggccgctg cgcccttgat gccctgcgag cttgcgtcgg agcttccgct gctttcgtca
     2281 gcttcttccg cagctttgtc gtcggcggag ggatccgtcg tctccgcatc cgcgtcgaga
     2341 ccggcgacct tttgctccga cgcttctgcg ttggtcttcg gtgtcgcctc gactgtcgga
     2401 gttacctcgg gcgctttgga gtttgcttcc tcgacctgaa ccgcgttcgc ctcgtgaacg
     2461 gacgtggcct cgatcggcgg caacgctcgt acgtcattca ctttcgggct tgccccagga
     2521 ggagcttggc gcgcggaaac caaagccgcg cggcgctccg cgagcctgtc ccacgcaccc
     2581 gttacggttt ggaggccctc actggcatgc tcacgcacca gcccggagag ccgtgggctc
     2641 cgagcaaacg ccgatccgag gaccgcaaag cagcccacca gcgtgacccc aaccgccacc
     2701 gctccgagtc ggcgccgaat cacgctccga cgtcgcggtt cgtgcagagc ggcttcgagc
     2761 tccttggcga gctgctcggc gctctctgga cggtcttcgg gagaaggtga cagagcacgc
     2821 aagatcgcat tttcgagcgc acgcggtagc tgcagtccct ccacgcggcg gctcgggcgc
     2881 tccgggcgct tttgcttctc cgcgatgatg gcgagcggag cgtctgcgtc gtgcggcaag
     2941 aacccggtac aaagctcgta cagtacggca ccgagcgcat agacgtcact gcgcgcgtcc
     3001 gactcgcccc gcgcttgctc cggggccatg tattcggggg tgcccaccag cgcgacggga
     3061 ttttcggcgt tcgggggcgc gtcagcggcc gtgatcgaag tggcgagccc aaagtccagc
     3121 accttcaggg taccgtcacg agtcacgaac aggtttgcag gcttgatgtc ccgatggatc
     3181 accttggcgc gatgtgcggc gtcgagggcc cggcaggctt gaattccgaa gtcgatgacc
     3241 gacgcggccg tcaggcgctg ctcgcgatcc aaccgctctg cgagcgtctg accgtcgagc
     3301 agctccatcg cgtagaacag gcgcccgtcg cggcacatgc cgaaatcgaa aaccttgact
     3361 aggttctcgt gtgacagctg cgcgatggct cgggcctcag cgcgaaaacg gctctgagcc
     3421 gcggcgctca tcgtctcggc ggagagcaac ttcaacgcga cagttcggct caagtcgacg
     3481 tgcacagcct ggtgcacgat gcccatcgcg ccgcgtccaa tctcacgcac cagccggtag
     3541 cgcgtaccgg acagcatgcc aggctccgtt gcttccggcg cctcgggcga cgcctcgggc
     3601 agcgggtgct tcggctcgag cgaacgagct tgagccaagc gcttgacgaa ttcaccgcga
     3661 ctacgagccg gctcggcggg atagagcctt cgcatcagat cggcgacgcg cgctcgaacg
     3721 ctgcgttcgc cgtcggctaa actggtcgcg cgctgcagga gcttgacgag gtcctgcgta
     3781 gctttgcgcg ccgactcata gcggtcctcg atgcgctgag cagtgagccg cgccaggatc
     3841 aaatcgagct ccgccggggc gccgatcaac ttggcgatgg gcaccaacgg gcggcgtccc
     3901 gcgcccacct cggtcagatg ctgcgtggct tccccggtca ggaagcggcg accggccaag
     3961 agctcccaaa gcatgacgcc gaaggcgtag agatccgccg gaatcccccc gggctgattg
     4021 ttcgcgacct cgggggccac gtagccgggc ttcgcgaaca cgataccgct gatggtttgg
     4081 cagcgccggt tttggcctcg agccgtgccg aaatcgatga tcttcagctc gccgccatag
     4141 cccaccatca cgttctgagg gctgaggtcg cgatgcacga tttcgagggg gcggccctcg
     4201 gcgtcggtgc gctcgtgcac gtgagccaga gcgtcgccca ggctcgtacc aatcgccacg
     4261 gcctcggccc agctgattcg aagcccgagt tggcccgagc gggtgcgtac gtcgcccaga
     4321 ttgcggccct cgacgtactc gacgacaacg taaggtttgc cttcggcgtc cgtggtcgcc
     4381 tccagaattt gggcgacgcc cgggtgctgg agttgagctt gaatgcgtgc ctcgtccaag
     4441 aagcgcgcga cgaacgaacg gtcctgcgcg tggtcgggcc gaatgatctt cacgatcagg
     4501 ggcctttcgg cgccctcgat gccgcgtgcc gccgccaggt agacctcgcc catcccccca
     4561 cgtgcgaccc gcgccagcag cgtaaagcgc tcgaagcggc gaggcagcgg gagatcgacc
     4621 tcttgcgggg cctgcgaacc cggattaatc tcttgcgatt tcaacgagtt atctgtgagc
     4681 tccagcggcg acatgtgggc atcctcggcg atgtaggcaa ctgtgggcaa ctattcgtca
     4741 gtcgaaagct cgggaatgcc ggccgcaggg ttctcgttga gctgggctgc gacgggatta
     4801 gactgccgcg ctcgccccat gatgcactgg tccttcacga gctggttcgt cgcgctcttc
     4861 gcgcgccgca tgggcttgtg cgtttggatc atggcgctca cgttttgcgt ctcgtcccgc
     4921 gcgtcggcgt tgacgagcgt gcccatgtgc ggcgagcacg ccgaaacgat cgcggccccg
     4981 ccaattagcc gcgcagcgaa agatgtaccg ctcagctgcg agcgaaagac cgcgcccagc
     5041 gccgtcgact cagccccgag caaagcgccc tcgccgccga gtacgagcct agaagcggcg
     5101 cctcgcctgc ctcccatcgt atatcgcttc ggccccgtgc ctcgggcgcg agtcccactc
     5161 gaagcaagct ccaacggtga actcgccggg tacgcgcgaa agttggagcg accgccgaga
     5221 aac
//
LOCUS       DASTKS010000808         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_351517, whole genome shotgun sequence.
ACCESSION   DASTKS010000808 DASTKS010000000
VERSION     DASTKS010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351517"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..2001)
                     /locus_tag="VFQ35_16735"
     CDS             complement(244..2001)
                     /locus_tag="VFQ35_16735"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET9932354.1"
                     /translation="MDLLTAGVQVELDADLELDRQQRAEALKAKLTRMLAEGKGAEAI
                     DQMMSLMLEMERTNDRLAWRVLLANRYRFGRSTEKLSRDELKQLYLAFGGDNAVAETQ
                     AELAVPAPAEPEQVDDRSAGTLESESESEPEGAASAEPAAPKKKTRQRVRSIQIAPHV
                     ERKEHTSLVPEEERVCALCGKEKKSFGCVEHQVITWVPAKIVVEVESREKLACQSCRK
                     DVSVAPRSQAPSVVRKVDASLLAKLVDEKCTLALPVDRQRRELARLGLDVPDKTLQSY
                     WAYTTDLIEPIAVATLSDVFGSCIVGVDDSHLKTLDKSRKHGVSRAHLWCFVGSGRSV
                     GGPESVAYGYTPSWDAAEIIDWFSAIEGFIQCDGYAGYSREVEDDDGQTLVAVPPERR
                     LGCGMHIRSKFHAALLAKDGRAAIPLKLFADLYKVEDDCKARGLDADARSEERRRRSA
                     PLLDVFDDWVDQLHPKLLPKSPLRRATTYAINQRPFFRRCFDDGRFEIDNGRVERRIR
                     LFAVARRNFLFTGSARGGERLAIAFTLVDNCLALGIEPHRYLVDVIGKLEAGWPMQRL
                     SELIPHRWATQHSAEKRAQ"
BASE COUNT          388 a          678 c          765 g          435 t
ORIGIN      
        1 ggaggagctc acatgattcc ggcaggcgtt cgcatcttcg tgtgtaccga gccagtcgac
       61 atgcgccaag gcttcgatcg tcttgcgcac actgctcgtg agcgcgtcgg tcacgatccg
      121 atcgacggcg gagcgtaagc cccttatggg gagcgtgatg gcgctgaacg cgagcgctac
      181 cagactgtcg gagcgatgtc ggagcagacc gagcagcgag tggcgccagc ggagactata
      241 ggcctactgg gcgcgcttct cggcggaatg ctgagtggcc cagcggtgcg ggatgagctc
      301 ggagaggcgt tgcatgggcc agcccgcctc gagcttgccg atgacgtcga cgaggtagcg
      361 gtgcggctcg atgccgagcg cgaggcaatt gtcgacgaga gtgaaggcaa tcgccagacg
      421 ctcaccgccg cgggcggagc ccgtgaagag gaagttgcgg cgagcgaccg cgaacagtct
      481 aattcggcgt tcgacgcgcc cgttgtcgat ctcgaagcgg ccgtcgtcga agcagcggcg
      541 gaagaacggg cgctgattga tagcgtaggt cgttgcgcgc cggagcgggg atttcggcag
      601 cagtttgggg tgaagctggt ccacccaatc atcgaacaca tcgagcagcg gtgccgagcg
      661 ccgccgccgt tcctcgctcc gagcgtcagc gtcgagcccg cgggctttgc agtcgtcttc
      721 gaccttgtac aagtcggcga agagtttgag cgggatggct gcgcgaccgt ctttcgcgag
      781 cagcgccgcg tggaacttgc tgcggatgtg catgccacaa ccgaggcggc gctcgggagg
      841 cacggcgaca agggtctgcc catcgtcgtc ttcgacttcg cgagagtagc cggcgtagcc
      901 gtcgcattga atgaagcctt cgatggccga gaaccagtcg atgatctccg ctgcgtccca
      961 gctcggggta tagccgtagg cgacggattc gggacctccg acactgcgac ccgagccgac
     1021 gaagcaccac aagtgagcgc gcgaaacgcc gtgtttgcgc gacttgtcga gggttttcag
     1081 gtgcgagtcg tccacgccaa cgatgcagga tccaaacaca tccgagaggg ttgcgacggc
     1141 aatgggctcg atcaggtctg tcgtgtaggc ccagtacgac tggagagtct tgtctggcac
     1201 gtcgagaccg aggcgtgcga gctcacggcg ttgacggtcg acgggcagtg cgagcgtgca
     1261 cttctcgtcg acgagcttcg cgagcagcga agcgtcgacc ttgcgcacca cgctcggcgc
     1321 ttggctgcgc ggcgcgaccg aaacgtcctt gcggcacgat tgacaagcga gcttttcgcg
     1381 gctctcgact tcaacgacga tcttggcggg gacccaggtg atgacttggt gctcgacgca
     1441 gccgaacgac ttcttttcct tgccgcagag cgcgcagact cgctcctcct ccggcacgag
     1501 agaggtgtgc tccttacgct cgacgtgggg agcgatttga atcgatcgaa cgcgctggcg
     1561 cgttttcttc ttcggcgcgg ctggctccgc gctagctgcg ccctccggct cggattcaga
     1621 ttcggactca agcgtgccgg cggatctgtc gtcgacttgc tcgggctcag ccggagccgg
     1681 aacagccagc tcggcctggg tttcggcgac ggcgttgtcg cccccgaacg ccagatagag
     1741 ttgtttgagc tcatcacgcg agagcttctc cgtgctgcgc ccgaagcgat aacgattggc
     1801 gagcagcact cgccacgcca gtcgatcgtt ggtgcgctcc atctccagca tgagcgacat
     1861 catttggtcg atcgcttcgg cacctttgcc ctctgcgagc atgcgcgtga gcttggcttt
     1921 cagcgcctcc gcgcgctgct gacggtcgag ctcgagatcc gcgtcgagct cgacctgcac
     1981 tccagccgtg agcaggtcca ttgagacggg cagcggtccc ggtgcaacga aggcgcgtgc
     2041 cttgctggca ctcttgttgg gctgtttttt aggctttcga cgcacgaggg acgtgcgccg
     2101 atatgatcaa tttcgatcgg gtggcgcaat agaattcgaa cgtgcggagc cgtttctact
     2161 cggcggcgtg atcaacgtgg ttagcgtcgt cgtgcttgta acgcttgcgc cgcctcgcac
     2221 ccgtgagctc gatgccttcc aacattagcg cgagctcggc ggagtc
//
LOCUS       DASTKS010000809         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_129505, whole genome shotgun sequence.
ACCESSION   DASTKS010000809 DASTKS010000000
VERSION     DASTKS010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129505"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..2087)
                     /gene="ftsH"
                     /locus_tag="VFQ35_16740"
     CDS             complement(144..2087)
                     /gene="ftsH"
                     /locus_tag="VFQ35_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9932355.1"
                     /translation="MKQPHKTLLLWVVLIFAFLAIWRFLNDADSNQKQDFAYSDFMAL
                     VKAPRDQRHIDEVEIKLNEHEYSFTIKNPNGKGQPEHGRTIGPGEDSAEELIKNNVRV
                     KFLRDDGGSLITPVLTILLPMVFVIVMFYLFMRQLQAGGGKAMSFGKSRARLLSESQN
                     KVTFADVAGVDEAKDEVEEIIAFLKDPKKFQRLGGRIPKGVLMMGPPGTGKTLLARAI
                     AGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRHRGAG
                     LGGGHDEREQTLNQLLVEMDGFESNEGVIIIAATNRPDVLDPAILRPGRFDRRITVPR
                     PDIRGREGILAVHTKKVPLSGDVDLSILAAGTPGFVGADLENLVNEAALLAARQDKDA
                     VSMEDFELAKDKVLMGSERRSMVMSEAERRTTAWHEAGHTIVGKLVEGNDAVHKVSII
                     PRGAALGVTMFLPTEDRHSMTRQQTLARIAMALGGRAAEEVVFGEITTGAQDDIKRAT
                     RLARAMVCELGMSSKLGPIAYGENEENVFLGREMSSRREDYSEDTAREIDQEVRAIVE
                     GQYRIAQSVLGANRDKLDRLASALLERETLDSEEIEATLAGRELPPRQRLIIPTYSEK
                     RNRDRSEKKRPASIFGAPKPATG"
     gene            complement(2256..>2707)
                     /locus_tag="VFQ35_16745"
     CDS             complement(2256..>2707)
                     /locus_tag="VFQ35_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932356.1"
                     /translation="VLIRLLRGTTARGLGVLPAQSGDLVRPLIRARRADIRAHAERHR
                     LRFAEDPSNRDPRFLRSRVRHELLPLLTALSPGIVENLNALADEQLNEALPPVVAADG
                     TRLSLGRAQRNELTRMLERPSLRACLRLSAGRKLYVDPRTRQIRVEE"
BASE COUNT          461 a          877 c          851 g          518 t
ORIGIN      
        1 tggtgcgagc ccaatgacgg ggcgctgcga tcgcgctcgc tcgcagcgat cactgtcgaa
       61 ccgcgagctc acaaaaaccg agagcgccgg cccagtgcgt tcactggact cggcgctctc
      121 agtcgcgctc ggcggagctt ggctcagccg gtggccggct tgggagcacc gaagatgctc
      181 gccgggcgct tcttctcgga gcggtcgcga ttgcgcttct ccgagtaggt cgggatgatc
      241 aagcgctgac gaggcggcaa ttcacgccca gcgagggttg cttcgatctc ctctgagtcg
      301 agcgtttcgc gctccagcaa cgccgaagcc aaccggtcca gcttgtcgcg attggcgccg
      361 agcacgctct gcgcgatgcg gtattgcccc tcgacgatgg cgcgcacttc ctgatcgatt
      421 tcgcgcgccg tatcctcgga gtagtcctcc cggcgcgaag acatctctcg gccgaggaac
      481 acgttctcct cgttctcgcc gtaggcgatg gggccgagct tcgagctcat gccgagctcg
      541 cacaccatgg cgcgagcgag gcgcgtggct cgcttgatgt cgtcctgcgc gccggtggtg
      601 atctcaccga acaccacctc ttccgcggcg cgtccaccga gggccatggc gatgcgcgcg
      661 agcgtttgct ggcgcgtcat cgagtggcgg tcctcggtcg gcaagaacat cgtgacgccc
      721 agggcggcgc cgcgcgggat gatcgacacc ttgtgcacgg cgtcgttacc ttcgacgagc
      781 ttgccgacga tggtgtggcc ggcctcgtgc caagcggtcg tacgacgctc ggcctcgctc
      841 atcaccatcg agcgacgctc gctgcccatc aggaccttgt ccttggcgag ctcgaagtcc
      901 tccatcgaga ccgcgtcctt gtcctgacgc gcggcgagca gcgccgcctc gttcacgagg
      961 ttctcgagat ccgcaccgac gaagccaggc gtgccggcgg cgaggatgct gaggtcgacg
     1021 tcaccggaga gcggcacctt cttggtgtgc acggcgagga tgccctcacg accgcggatg
     1081 tccggccgcg gcacggtgat gcggcggtcg aagcggcccg gacgcaagat agccgggtcg
     1141 agcacgtccg gacggttggt cgcggcgatg atgatgacgc cctcgttgga ctcgaagccg
     1201 tccatctcga cgagcagctg gttcagcgtc tgctcgcgtt cgtcgtggcc accgccgaga
     1261 ccggctccgc ggtgacgacc caccgcgtcg atttcgtcga tgaagatgat gcagggcgcg
     1321 tgcttcttgc cctgctcgaa gaggtcgcgg acgcggctcg cgccgacacc cacgaacatc
     1381 tcgacgaaat cggagccgga gatgctgaag aacgggacac ccgcttcgcc ggcgatggcg
     1441 cgtgccagca aggtcttccc ggttccgggc ggacccatca tgagcacgcc cttcgggatg
     1501 cgaccgccga ggcgctggaa cttcttcggg tccttcagga aggcgatgat ctcttcgacc
     1561 tcgtccttcg cctcgtcgac gccggcgacg tccgcgaacg taaccttgtt ctgtgactcg
     1621 ctgagcaggc gcgctcggct cttaccgaag ctcatcgcct tgcctccacc tgcctgcagc
     1681 tgccgcatga acaggtagaa catcacgatc acgaacacca tcggcaacag aatggtgaga
     1741 acgggcgtga tcaatgagcc tccatcgtcg cgcaagaact tgacgcgcac gttgttcttg
     1801 atgagctctt cggcgctgtc ctcaccgggc ccaatggttc ttccgtgctc gggctggccc
     1861 ttcccgttcg ggttcttgat cgtgaacgag tactcatgct cattgagctt gatctcgacc
     1921 tcgtcgatgt gacgctgatc gcgaggcgcc ttcacgagcg ccatgaaatc gctgtacgcg
     1981 aagtcttgct tctggttgct atctgcgtcg ttcaaaaatc gccagatcgc gaggaacgcg
     2041 aaaatcagaa cgacccagag caaaagtgtt ttgtgcggct gcttcacccg aaggacctca
     2101 atgcacggtt gaacgacctc gatgcctgcc tattgacaag catctcaacc gaagcatgcc
     2161 acccttcccg tgcttcgtcg atggggcgca cttcaataat ttgtgatttt cggggcttat
     2221 ctcgcgtctc gagcggtcgc cgcgagagcg cacactcatt cttccacgcg aatctgacga
     2281 gttcgtgggt cgacatacag ctttcttccc gccgaaaggc gtaaacacgc gcgcagactc
     2341 gggcgttcga gcattcgtgt aagctcgttt cgctgcgccc tgcccaagct aaggcgtgtt
     2401 ccgtcggctg caaccactgg cgggagcgct tcgttcagct gttcgtcggc gagcgcgttc
     2461 aggttctcta cgatgcccgg cgaaagcgcc gtgaggagcg gcaagagctc atggcgcacg
     2521 cgcgagcgta aaaagcgcgg gtcgcggttc gaaggatctt ccgcaaagcg tagccgatgg
     2581 cgctcggcgt gcgctcgaat gtcggcgcga cgcgctcgaa tcaggggccg taccaaatcg
     2641 ccgctctgcg ccggaagtac tcccagcccg cgcgccgtag tgccgcgcag caagcggatc
     2701 agcaccg
//
LOCUS       DASTKS010000810         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_797802, whole genome shotgun sequence.
ACCESSION   DASTKS010000810 DASTKS010000000
VERSION     DASTKS010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797802"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFQ35_16750"
     CDS             <1..464
                     /locus_tag="VFQ35_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932357.1"
                     /translation="TRDEFRAEVWLAIIHGARGIFYFAHSDCVAGPCTTPDGMTADLV
                     DEMTIQNARISGIASVLQSPINPSTIGFAPGKSAVNLPLEATWRQSGGKSYLIVANAS
                     PNPLTAAPMVVSGLPAGTSALTVHGENRSVSLASGQFSDSFQPYAVHIYRN"
     gene            824..2029
                     /locus_tag="VFQ35_16755"
     CDS             824..2029
                     /locus_tag="VFQ35_16755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9932358.1"
                     /translation="MIGRSRKAEVLLADPGVSGVHACIEPRSGGAFVRDLGSRNGTFA
                     NGERVTSAGAALRSGEVLRVGDTLLLLVDDVELYRTRPRRVEAATLGIAEPVVAGPAL
                     ARVWDEATRAAQLRHPVLLLGESGSGKECIARMLHAARAEGGPFVGINMAAVPDSLFE
                     AELFGHERGAFTGARSARLGAFREATGGVLFLDEVGELNPPLQAKLLRTLDQRRVRPL
                     GADREYEVNVQLVSATNRELKSASEDGNEAFRTDLYYRLAGIVIRVPPLRERPDDIIL
                     LTEQLLRAQRPQLKLSADVAELLVLGRWEGNARELRYAVVHATLAALASGSDELRAEH
                     VKAPLPKAAGGGPLTAERMRAALAQCDGVATHAARALGVSRATFYVALKRFNLGSQKL
                     ARGSNLDGT"
     gene            2026..3567
                     /locus_tag="VFQ35_16760"
     CDS             2026..3567
                     /locus_tag="VFQ35_16760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932359.1"
                     /translation="MIADPFGLIGTIVDGRYRVERVAGQGGFGIVYRAHHLGFESPIA
                     LKILKLPERWTSAARTARIANFQREGRMLFELSLIHPSIVRAFETGTVTGPDGSPAPY
                     LALEWLDGVSLEHEIKLRRRKQLPPLSLHQVLNLLSAPAQGLARAHARGIAHRDIKPG
                     NLFLSVREAEPSVKILDFGIAKAIDETSSTSEQFAETDGLATSFTPMYAAPEQWLRRL
                     GATGTWTDVHALALVCVELLSGRSPFSGGESAQFMAACLDPQRRPTPGAAGVRLSPSV
                     EAVFARAVSLEPRSRCRDIGEFWSALCSAAGWSESEALAPAFMGSISELSLAELTSAP
                     ASRAVAPSSSTTAATAVRVSGYQVAQRLRLSRRHALVIAASAVAVAGVAVRALPEPPQ
                     SPPQAPSRHVLPAVTSPLETRIATATAASSGAQIASTPSAEAGASLSEPEPSAARLRA
                     VERTATRAPAKRIEPASSAKPADSGPQTTPPDHDTTRALAPAEPATQLGPEMDLDDPA
                     LAKRR"
     gene            3659..>3871
                     /locus_tag="VFQ35_16765"
     CDS             3659..>3871
                     /locus_tag="VFQ35_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932360.1"
                     /translation="MTAALAAAGSAYADPNLTPSERAQQLFDAGRVALKEHRLEEACA
                     DLTESARLDAAAGTWLNLAACREAQGR"
BASE COUNT          648 a         1268 c         1279 g          676 t
ORIGIN      
        1 ccactcgaga cgagtttcgc gcggaagtgt ggctcgccat cattcacggc gcgcgaggga
       61 tcttctactt cgcccattcg gactgcgttg ccggaccttg cacgaccccc gatggcatga
      121 ctgcagatct ggtagacgag atgaccatcc agaatgctcg catcagcggg atcgcgtcag
      181 tcttacagtc cccgatcaac ccctcgacga tagggtttgc accgggcaag tctgcggtga
      241 acctgcctct cgaagcgacg tggcggcagt cgggcggcaa gtcctatcta atcgtagcga
      301 acgcctcgcc gaacccccta accgccgcgc cgatggtagt aagtgggctg cccgcgggaa
      361 caagcgcgct cacggtgcac ggagagaatc ggtcggtgtc gctcgcatcg ggtcaattca
      421 gcgattcctt tcagccgtac gcagtacata tctatcggaa ctgaaagtgg ctcgattcta
      481 catcacacga taagtcgcat cctgcggaga agagcgaccg ctggccgagg agccggggat
      541 tcgggggcaa ttgggggtct tctccaattg cccgattggc cctgaagctc gtttttgcgg
      601 tcggggtggt tgaacggcgt gagagcgtgt ggcagagtct cgcgagtgag ggtgtggcga
      661 ggggagtgca gtggtgggga tgaatgcttc gcggactgct ccgacgtctg accgtggcgc
      721 gtcttcgctc gatgctcgag cgatattgcg accagggctc gtggcaattc atccaatccg
      781 agatgacgaa gccccgcccg tatggacgct caagcgcacc gccgtgatcg gtcggagcag
      841 gaaggccgaa gttctcttgg ccgatccggg cgtctccggc gttcacgcct gcatcgagcc
      901 tcgctcggga ggcgcgttcg ttcgagatct gggaagccgc aatggtacct ttgcaaacgg
      961 tgagcgcgtc acttcggccg gagccgcctt gcgcagcggc gaggtattgc gagtgggcga
     1021 tacgctgctg ctcctcgtcg acgacgtaga actctacaga accaggcccc gccgcgtgga
     1081 agcagcgacg ctcgggatcg cggagccggt cgtcgcgggg cccgcgctcg ctcgggtttg
     1141 ggatgaagcg acacgtgccg cgcagctccg tcatcccgtc ctgctgctcg gcgaaagcgg
     1201 cagcggaaag gaatgcatcg cgcgcatgct gcacgcggcc cgtgccgagg gaggtccctt
     1261 cgtcggcatc aacatggccg ccgttcccga ttctctgttc gaggccgagc tttttggtca
     1321 cgagcgcggg gcgttcaccg gagcgcgaag cgcgcggctt ggtgcctttc gcgaggccac
     1381 gggcggggtg ctcttcttgg acgaggtggg tgagctgaat ccgccgctgc aggcgaagct
     1441 cctgcgcact ctcgatcagc ggcgtgttcg tccgctcggc gccgatcgag agtacgaggt
     1501 gaacgtgcag ctcgtgagcg ccaccaaccg cgaattgaaa tctgcgagcg aggacgggaa
     1561 cgaggcgttt cgtacggatc tgtactaccg cttggcgggc atagtcatcc gcgtgccacc
     1621 gctgcgggaa cgtccggacg acatcatcct tttgaccgag cagttgttgc gagcacaacg
     1681 cccgcaacta aaactctcgg cggacgtcgc cgagctcttg gtgctcggtc gctgggaggg
     1741 caacgctcgc gagttgcgct acgccgtggt tcacgcaact ctcgccgcgc tggccagcgg
     1801 gagcgacgag ttgcgcgcgg agcatgtcaa ggctccgctg ccgaaggccg ccgggggcgg
     1861 gccgctcacc gccgaacgca tgcgggcggc gctagcgcag tgcgacggcg tcgccacgca
     1921 cgcggctcgc gcattgggcg tatcgcgagc gaccttctat gtggcgttga agcgcttcaa
     1981 tctcggctcg cagaaactag ctcgcggctc gaacctcgac ggaacatgat cgcggatccc
     2041 ttcgggctca tcgggaccat cgtggacggt cgctaccgcg tcgagcgcgt ggctgggcaa
     2101 ggtgggttcg ggatcgtcta tcgcgcgcac cacctcggat tcgaatcgcc catcgcactc
     2161 aagatcctga agctgcccga gcgttggacg agcgccgcaa ggaccgcgcg catcgcgaat
     2221 tttcagcgcg aagggcggat gctgttcgag ctgtcgttga tccatccgag tatcgtccgc
     2281 gcattcgaga cggggacggt tacggggcct gatggctcgc cagcgcctta cctcgcgctc
     2341 gaatggctcg atggcgtgtc gctggagcat gaaatcaagc ttcgccgccg aaagcagctg
     2401 ccgcccctgt cactgcacca agtgctgaac ctgctttcgg cccctgccca aggtttggct
     2461 cgggcgcacg cccgcggcat cgcgcatcgc gacatcaagc cgggcaactt gttcctgagc
     2521 gttcgcgaag ccgagcccag cgtgaagatc ctagattttg gcatcgccaa agcaatcgac
     2581 gaaacctcga gcacgagcga acagtttgcg gaaaccgacg gtttggccac gtcgtttacg
     2641 cccatgtatg cggctcccga acagtggctt cgccgcctgg gcgcgacggg cacgtggacc
     2701 gacgtgcacg cattggctct cgtctgcgtc gagctcttga gcgggcgcag tcccttctcg
     2761 ggtggggaat ccgcgcaatt catggcggcg tgcctcgatc ctcaacggcg cccgacgccg
     2821 ggagccgcgg gcgtgcgact gtcgccgagc gtagaggctg tatttgctcg cgccgtttcg
     2881 ctcgaaccgc ggagccgctg ccgcgacatc ggtgagttct ggtcggcgct atgcagcgcc
     2941 gcgggttggt ccgagagcga ggccctcgcg cccgcgttca tgggatcgat ctccgagctt
     3001 tcgttggcgg aactcacgag cgcccccgcg agccgagcgg tcgcgcccag ttcgagcacg
     3061 accgccgcaa cagccgtgcg cgtgagcggc taccaggtgg cacagcgact tcgcctgagt
     3121 cgccgacacg ccttggtcat cgcggcgtcc gcagtcgcgg tcgcaggtgt cgccgtgcgc
     3181 gcgctaccgg agccgcccca gtcgccacct caggccccct cgcggcacgt cctaccggct
     3241 gtgacgtccc cgctcgagac gcgaattgcc accgccaccg cggcttcgtc cggggcgcaa
     3301 atcgcctcca caccttcggc cgaggccggc gcctcgctct ccgaaccgga gcccagcgcg
     3361 gctcgcctgc gcgctgtcga gcgcaccgcg acgcgagccc cggcgaagcg aatagagccg
     3421 gcatcttcag ccaagcccgc cgactcgggg ccgcaaacga cgccgccgga tcacgacact
     3481 accagggcgc tcgcgccggc cgaacctgcg acgcaactcg gcccagaaat ggatttggat
     3541 gacccagccc tcgcaaagcg tcgctagtac acgtcgccaa aaaggtgccg cgctctctca
     3601 gcgcggccct cgacgttcat cctggccgac gcgtcaccga ctcgttatcg ggtgctgggt
     3661 gacagcggct ttggctgcgg caggctcggc ctacgctgac cccaacctca ctccgagcga
     3721 acgcgcccaa cagctcttcg atgcgggtcg cgtcgccctc aaggagcatc gtttggagga
     3781 agcctgcgcc gacctgacag agagtgctcg tttggacgca gctgccggga catggctcaa
     3841 cctggccgcc tgccgcgagg cccaaggccg c
//
LOCUS       DASTKS010000811         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_798006, whole genome shotgun sequence.
ACCESSION   DASTKS010000811 DASTKS010000000
VERSION     DASTKS010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798006"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..917)
                     /locus_tag="VFQ35_16770"
     CDS             complement(27..917)
                     /locus_tag="VFQ35_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9932361.1"
                     /translation="MCRTILDVAARAISSGTISFGLVSIPVKLYTAASSEQVSFNMLH
                     TKCKGRIKMQYFCPTDNEVVERSDTLKGYEYAKGQYVLFTEEELKALEAARDNSIEIT
                     EFVPLSSVDLVQVEKSYYLGPDKGGDKAYRLLSEAMDGKDRVAVGRWAARGKEQLVLV
                     RPHGDGGLILHQLYYANEVRSFEDIETGAKFSFSDKERELADKLIEQLSSEAFEAEKY
                     HDSYSDRVLAAVDQRVQGQEVTVAPEAPKAQIIDLFEALKKSLENAPKPFEPGTGAIK
                     PPKKAPAKPKSEGKKTKASG"
     gene            982..3579
                     /gene="ligD"
                     /locus_tag="VFQ35_16775"
     CDS             982..3579
                     /gene="ligD"
                     /locus_tag="VFQ35_16775"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET9932362.1"
                     /translation="MPKPPPSKQPASGVSAASSDALAEYRHKRNPLSTNEPFAAERQR
                     RTGATRNGRFVVHLHSARNRHYDLRMQVGRRLVSFPVPRGPSLDPREKRLAIQTEDHP
                     LEYADFEEIIPEGNYGAGPMIAWDIGRVSYLETTAEEGLVAGKLDFVLYGHKLRGRFA
                     LVRTKRGAGNEWLLIKKPDAYSREEGDILEDSPESVLSGLTVDELALRDAIAAETAAR
                     ARQAGAVDRQLAASFEPMLCSDEGGRIDDPERLYELKLDGVRIVARKSGRKVSLTYRN
                     GGVCTHNYPEVARALETIPGNEVVLDGEIIAFDEHGRPRFQRLGPRIQARTALDVARV
                     AAETPVVMLVFDLLALDGLDLTQLPLVTRKSLMKELVRGKGLVRALDHFEGRGAELFE
                     LCKAQGLEGVVGKQRNSVYRFGPRRGLEWVKFKCEREDDFVVIGYIPGKGTRGKIGAL
                     GLGTYRDGVLTYRGRVGTGFDGKMLDFFAKEIGARETKERTIPAELPEDSRQMRWVRP
                     ELVVNVRYQGYTDDARLRFPIFQGVRADIAPSSCLAGPHDEANGPLVFEAPEQKAATP
                     ERAQAVVKLSNRGKVFWPEQGYTKGDLLDYYASVAHVMLPFLEERPVVLVRYPDGIHG
                     KNFYQWNVPQGTPEWLRVLVLQDPEEPDKQKHVFLLDSVEALLHVINLGCIPLHVLAS
                     RAGTPELCDFITFDFDLGSQPFARAIELALRLKNVLEDTGLVGFPKTSGQEGLHVLVP
                     LGPGVPFTAAKLMVELLGRIVVSTNESFSTMERRVDKRGGRLYVDTGQTGTSRTIVAP
                     YSVRANAIAGVSTPLFWDELSGALDVQRFNLMTVPARIEELGDPWSALLEARPDIPGA
                     LAKLEKWLR"
     gene            complement(3646..>5069)
                     /locus_tag="VFQ35_16780"
     CDS             complement(3646..>5069)
                     /locus_tag="VFQ35_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932363.1"
                     /translation="GGASSGGKTSQGGNPSTGGASGGEGGSNPVQGGSAGSEPMTGGA
                     GGWSSSEGGAGGMAGGGGEGGVPVEQITSDGYSIGAFAPLANYTGISGQALIARKRGG
                     GTHVSVQVIGLQPSAQYPVHVHAAPCSQSAGGGHYKRDPSVTTTSEANELWVPVTTDA
                     NGVGIGWLDVPTHEARGDALSVVVHDPNATDTPKMACADLSIQSATPLQASGKFGSFA
                     QATTVDTPIAGTAWLVRNTASTLVSFDVKGLDPAAMYSAHIHALPCAVTAAGGHYKID
                     TTITATQETNELWVPLGDTSTGSASGSLNFAHAARSDAQSMVIHRMDGANALKVACAD
                     LAVSVYPPLRMAGVAVPFPAATDLGYPSLAATAAMTREQDQSMRFELTVSGLAEHSVY
                     PTHVHNLPCALQSGGGHYKIDPTIAATEQSNEIWLNFTAGHEGGGSFRTRGSTLLRAD
                     ARSIVIHDSATAATRLACIDLAP"
BASE COUNT          828 a         1647 c         1638 g          956 t
ORIGIN      
        1 gcaacccgtc cgacgttcac gtttgctcag ccgctcgcct tggtcttctt gccctcgctc
       61 ttgggcttcg cgggggcctt cttcggcggc ttgatggcgc cggttcctgg ctcgaacggc
      121 ttgggcgcgt tctcgaggct cttcttcagc gcctcgaaca ggtcgatgat ctgtgccttg
      181 ggcgcctcgg gcgctaccgt gacctcttgc ccttgcacgc gttggtcgac ggcggcaagc
      241 actcggtcgc tgtacgaatc gtggtacttc tccgcttcga acgcctcact cgagagttgt
      301 tcgatgagct tgtcggcgag ctcccgctct ttgtcgctga agctgaactt cgcgcccgtc
      361 tcgatgtctt cgaaggagcg cacttcgttc gcgtagtaga gctggtgaag gatgagcccc
      421 ccgtcgccgt gggggcgcac caagacgagc tgctctttgc cgcgcgcggc ccagcgcccg
      481 acggcgactc gatccttgcc gtccatcgcc tcgctgagga ggcggtacgc cttgtcgccg
      541 cccttgtcgg gcccaaggta gtacgatttt tcgacttgca cgaggtcgac gctcgagagc
      601 ggcacgaact cggtgatctc gatgctgttg tcgcgcgccg cttcgagcgc cttcagctct
      661 tcttcggtga agagcacgta ctgccctttg gcgtactcgt agcccttcaa ggtgtcgctc
      721 cgctcgacca cctcgttgtc ggtggggcag aagtactgca tcttgatcct ccccttgcac
      781 ttggtgtgca gcatgttgaa gctcacctgc tccgaggagg cagcggtata gagcttcacc
      841 gggatcgaga cgagaccgaa cgaaatggtg cctgaagaga tggcgcgtgc agccacgtcc
      901 aggatggtac ggcacatatt cggttcttgg aaattacacg cgaagcattg gctcgccggt
      961 ccgctatagt tcgataaggc tttgcccaaa cctcctcctt cgaagcagcc agccagtggc
     1021 gtttctgcag cgagttcgga cgcgctcgcc gagtatcggc acaagcgcaa cccgctcagc
     1081 acgaacgaac cgtttgccgc tgaacgccag cgcaggactg gcgcaacccg caacggtcgc
     1141 ttcgttgttc atttgcacag tgctcggaac cgccactacg acttacgtat gcaggtcggg
     1201 cggcgcctgg tgagctttcc cgtaccgcgc ggtccgagcc tcgatccccg agaaaagcgc
     1261 ctcgcgatcc agaccgagga ccaccccctc gagtacgccg acttcgagga aatcatcccc
     1321 gagggtaact acggcgccgg ccccatgatc gcgtgggaca tcggacgggt gagctacctc
     1381 gagaccacgg cggaggaggg gttggtcgcg ggcaagctcg acttcgtgct gtacggccac
     1441 aagctgcgag gccgcttcgc cctcgtccgc acgaagcgcg gcgccggcaa cgagtggctc
     1501 ctcatcaaaa agcccgacgc ctactcgcgc gaggaaggcg acatcttgga ggattcgccc
     1561 gagagcgtgc tcagcggcct cacggtcgac gagttggcgc tgcgggatgc gatcgccgcc
     1621 gaaaccgcgg cgcgagccag gcaagcaggc gcggtcgacc ggcagctcgc ggcctcgttc
     1681 gagcccatgc tgtgctcgga cgagggcggc agaatcgacg acccggagcg cttgtacgag
     1741 ctcaagctcg acggcgtgcg catcgtcgct cgcaagagcg gtcgtaaggt gagcctcact
     1801 taccgcaacg gtggcgtgtg cactcacaac tatccggagg tggcccgcgc gctcgaaacc
     1861 atccctggca acgaggtcgt gctcgacggc gagatcatcg cgttcgatga gcacggccgg
     1921 cctcgctttc agcgcctcgg gccccgcatt caagcgcgca ccgcgctcga cgtggcgcgg
     1981 gtagccgctg agacgccggt cgtcatgctc gtgttcgact tgctcgcgct cgacggcttg
     2041 gatttgacgc agcttccgct cgtaacgcgc aagagcttga tgaaggagct cgtgcgcggg
     2101 aagggcctcg tgcgtgcgct cgatcacttc gaaggccgcg gcgccgagct cttcgagctc
     2161 tgtaaagccc agggtctcga aggggtcgta ggcaagcaac ggaactcggt ttaccgcttc
     2221 ggcccgaggc gcggccttga gtgggtcaaa ttcaaatgtg agcgcgaaga cgacttcgtg
     2281 gtcatcggct acatcccggg caaaggcacg cggggaaaaa tcggcgcgct cgggctcggt
     2341 acttatcgcg acggcgtgct cacctatcgc gggcgggtcg gtactggctt cgacggcaag
     2401 atgctcgact tctttgccaa agagatcggc gcgcgcgaaa ccaaagagcg aacgattcct
     2461 gccgagctgc ccgaggactc acgccagatg cgctgggtgc ggcccgagct cgtcgtgaac
     2521 gtgcgctacc agggatacac cgacgacgcg cgccttcgct tcccgatctt ccaaggggtg
     2581 cgggccgaca tcgcaccgag ttcgtgcctc gccgggcctc acgacgaagc caatggtccg
     2641 ctcgtcttcg aagcccctga gcaaaaggcg gcgacgcccg aaagggcgca ggccgtcgtc
     2701 aagctcagca accggggcaa ggttttctgg cccgagcagg gctacacgaa aggggatctg
     2761 ctcgattact acgcgagcgt cgctcacgtg atgctgccgt tcttggagga acgcccggta
     2821 gtcttggtgc gctatcccga tggcatccac ggcaagaact tttaccagtg gaacgtcccg
     2881 caaggcacgc cggaatggct gcgcgttctc gtcctccaag accctgaaga gccggacaaa
     2941 cagaagcacg tcttcttgct cgactcggtc gaagcgttgc tgcacgtcat caatctcggc
     3001 tgtatcccgc tgcacgtcct cgcgagccgt gccggaacgc cggagctttg cgacttcatc
     3061 accttcgact tcgatttggg gagccaaccc tttgctcgcg ccatcgagct tgcgctgcgg
     3121 ttgaagaacg tgctcgaaga cacgggcctc gtcggctttc cgaagacttc gggccaagag
     3181 ggcctgcacg tgctcgtgcc gctcggcccg ggcgttccct tcacggccgc gaagctcatg
     3241 gtcgaattgc tcgggcgcat cgtggtgagc acgaacgaat cgttttcgac catggagcgt
     3301 cgtgtggaca agcgcggcgg gcgcctttac gtggatacgg gccagaccgg cacgtcgcgc
     3361 accatcgtcg ctccgtactc ggtacgcgcg aacgccattg ccggcgtttc gacgccgctc
     3421 ttttgggacg agctctccgg tgcgctcgac gtgcagcgct tcaacctcat gaccgtgccg
     3481 gcgcgcatcg aagagctggg cgacccatgg tcggctctcc tcgaagcgcg cccagacatt
     3541 ccgggcgcgc tcgcgaagtt ggagaagtgg ctcaggtgag gtgagggcgc gccattagct
     3601 ctgcgctcgt ggcgcgcttt cggtacgggg tagagggcgc cgagcctacg gggccagatc
     3661 gatgcaggcg agccgtgtcg ccgcggtcgc cgagtcgtga atcacgatcg aacgcgcgtc
     3721 cgcgcggagc agcgtgctcc cgcgtgttcg gaaagagccg ccaccttcgt gcccagccgt
     3781 gaagttcagc caaatttcgt tagattgctc cgtggccgcg atggtcggat cgatcttgta
     3841 gtgtccgccg ccactttgca gtgcgcaggg caggttgtgg acgtgagtcg gatacacgct
     3901 gtgctccgcg agcccgctca ccgtgagctc gaagcgcatg ctctggtctt gctcgcgcgt
     3961 catggccgcg gtcgccgcga gcgatgggta accaaggtcg gttgcggccg ggaacggcac
     4021 ggccacgccc gccattcgga gcggaggata tacggacacc gcgagatcgg cgcacgccac
     4081 cttcaaagca ttcgcgccgt ccatccggtg gatcaccatc gactgagcgt cgcttcgcgc
     4141 ggcgtgggcg aagttcagcg agccgctcgc gctgcccgtg ctcgtatcgc cgagcggaac
     4201 ccagagctcg ttcgtctcct gagtcgcagt gatggtggta tcgattttgt agtgccctcc
     4261 tgcggcggtg acggcgcaag gtagcgcgtg aatgtgcgcc gagtacatcg ccgcgggatc
     4321 gagccccttg acgtcgaagc tgacgagcgt cgaagccgtg ttgcgcacga gccaggcggt
     4381 cccggcgatg ggagtgtcca cggtggtggc ctgcgcaaag ctcccgaatt tgccgctcgc
     4441 ttgcaggggc gtagccgact gaatcgacaa gtcggcgcac gccatcttgg gagtgtcggt
     4501 ggcgttcggg tcatgcacca cgaccgagag cgcgtccccg cgggcttcgt gggtgggcac
     4561 gtcgagccag ccgatgccca cgccgttggc gtcggtcgtg actggaaccc aaagctcatt
     4621 cgcttcgctc gtcgtcgtta cgctcggatc gcgcttgtag tgaccgccgc ccgcgctctg
     4681 gctgcagggc gccgcgtgaa cgtgaacggg atactgcgcg ctcggctgta gcccgatgac
     4741 ctggacggag acatgggtgc cgccgccacg cttccgcgca atgagggctt gcccgctgat
     4801 gccggtgtaa ttcgcaaggg gagcgaacgc gccgatcgag tacccgtcgc tcgttatctg
     4861 ctcgaccggg acgccgcctt cccctccgcc ccctgccatg cctcccgcgc cgccttcgct
     4921 cgagctccaa ccgccggctc cacccgtcat cggctcgctg cccgcgcttc cgccctgaac
     4981 ggggttgctg ccgccttctc cgccggaagc tccgccggta gaaggattcc cgccttgtga
     5041 agtctttccg ccgctcgatg ctccgcccg
//
LOCUS       DASTKS010000812         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_798334, whole genome shotgun sequence.
ACCESSION   DASTKS010000812 DASTKS010000000
VERSION     DASTKS010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798334"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1282)
                     /locus_tag="VFQ35_16785"
     CDS             complement(38..1282)
                     /locus_tag="VFQ35_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9932364.1"
                     /translation="MQVSAFRPVPRTGVIYVTVEAQRRGFRAEDPDWCNLGQGQPETG
                     ALPGAPPRVEHLEISEFNQEYAPVAGLWEVREAVANLYNDLFRRGMPSKYSAENVAIC
                     GGGRVSIMRACAAIGQIHLGHFLPDYTAYEELLDIFRRFTPIPILLDPARGYDFSLAE
                     LRREIGGRGLGALLLSNPCNPTGKAIVGPELESWVATARELECALLVDEFYSHYLWGD
                     GPPLASAAEFVEDVEKDPVVIFDGLTKNWRYPGWRVTWTVGPKHVIESLASAGSFLDG
                     GGSRPMQHAALQVLTRDHALREAEAIRQTFRRKRDKLIDGLRSIGVRLEREPEGTFYV
                     WGNLSNLPAPLDTGEGLFQAALEQKVITVPGQFFDVDPGQRRVGRASRFRHHARFSFG
                     PAESVIDTAIGRLKKIVADAKK"
     gene            complement(1313..2263)
                     /locus_tag="VFQ35_16790"
     CDS             complement(1313..2263)
                     /locus_tag="VFQ35_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9932365.1"
                     /translation="MTEPLLSVRDLRVHFPSPAGLVRAVDGVSLDLYPGETLALVGES
                     GCGKSTLARALVGLVPSSGGSVKLEQREIAKLGRAALRPLRKQLQLVFQDPYASLNPR
                     LSIGSALAEAVSLHRLLTPSELDTRVAELLSQVGLDPKLRDRYPHEFSGGQRQRISVA
                     RALAVEPRILLCDEVTSALDVSVQAQLIELLKGLQSSLGLSYLFITHDLGVVRAVAQR
                     VAVMYLGQIVEVQPTEAFFEEPEHPYSEALLASVPRLRPEVVERALLRGEVPSPANPP
                     FGCRFHPRCPARFDRCDKEAPELYPLEQGVSRCFLCDRSA"
     gene            complement(2260..3252)
                     /locus_tag="VFQ35_16795"
     CDS             complement(2260..3252)
                     /locus_tag="VFQ35_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9932366.1"
                     /translation="MSLLSVRNLTVEFTNGEEGVRAVDDLGFEIEPGEIVGLVGESGS
                     GKSVSTLAVLGLLEGRGVITRGSAWFGGEDLMKLSSERLRRVRGRRIALVFQDPLTSL
                     NPYLTLDEQMCEVAEVHLGLSRKAARSRAVELLTRVGIAEAEKRLRGYPHQLSGGMRQ
                     RVCIAMALLCGPDLLIADEPTTMLDVTIQAQILELLRELRRERNMSLLFITHDLGVVA
                     ELADRVVVMYAGRVVEEALTQDLFREPLHPYTEALLKSTPRIDQAGGGKLSPIGGFPP
                     RLGLQPVTECTFAPRCSRVREGCRHGEPKLFTLATTRRRRCIAPVEEMQSERAP"
     gene            complement(3328..>3796)
                     /locus_tag="VFQ35_16800"
     CDS             complement(3328..>3796)
                     /locus_tag="VFQ35_16800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9932367.1"
                     /translation="VILIMLLFAESARGDPLPVFLALGLVQWLTMARIVRGQVISLRH
                     REFVAAAELLGASDARILLRHILPNVLGVVIVYATLMVPSVIILEAFLSFLGLGVALS
                     WGQLVADGVAVVNPVHSFWWILLWPSLFLGLTLLSLNFLGDGLRDALDPKSRE"
BASE COUNT          709 a         1219 c         1260 g          608 t
ORIGIN      
        1 tcgggagcgc gtgcagcacc gcctcgggcg cgggccttca cttcttcgcg tcggctacga
       61 ttttcttcag gcgcccgatc gcggtgtcga tgacgctttc ggccgggcca aacgagaaac
      121 gcgcgtggtg cctgaagcga gaagcgcgac ccacgcgacg ctgtccgggg tcgacgtcga
      181 agaactgacc cggaaccgtg atgacctttt gttcgagcgc ggcttggaac agtccctctc
      241 cggtgtcgag cggcgccggc aagttcgaga ggttgcccca cacgtaaaag gtgccttccg
      301 gctctcgctc gaggcgcaca ccgatgctgc gcagcccgtc gatgagcttg tcgcgcttgc
      361 gccgaaaggt ctgacgaatc gcctccgcct cgcgcagggc gtggtcgcgc gtcaacactt
      421 ggagcgcggc gtgctgcatc ggtcgcgacc ccccgccgtc gaggaacgat cccgcgctcg
      481 ccagggactc gatcacgtgc ttgggtccga ccgtccaggt cactcgccag ccggggtatc
      541 gccagttctt cgtgagcccg tcgaagatca cgaccggatc cttttccacg tcttcgacga
      601 attcggctgc gctcgccagg ggcggaccat caccccagag gtaatgtgag tagaactcgt
      661 cgacgagcag cgcgcactcg agctcgcgcg ctgtcgcgac ccacgactcg agctccggac
      721 caacgatcgc cttgccggtc gggttgcaag ggttcgacag caacaacgct cccaagccgc
      781 gccccccgat ctcgcggcgg agctcggcga gtgagaagtc gtagccacgc gccgggtcca
      841 gcaaaatcgg gatcggcgtg aagcggcgaa aaatgtcgag gagctcttcg tacgcggtgt
      901 agtccggcaa gaagtggccg agatggatct gcccgatggc cgcgcacgca cgcatgatcg
      961 agacgcgtcc gcccccacag atcgcgacgt tctccgcgga gtacttcgaa ggcatgccgc
     1021 gccgaaagag gtcattgtag aggttcgcga ccgcttcgcg cacttcccac aaaccagcga
     1081 ccggcgcgta ctcttggttg aactcgctga tctcgaggtg ctcgacgcgc ggcggggcgc
     1141 cgggtagcgc acccgtctcc ggttgacctt gtccgaggtt gcaccaatcc ggatcttctg
     1201 cgcggaagcc acggcgctgg gcttcgaccg tcacgtagat gaccccggtg cgcggaacag
     1261 gacgaaacgc gctcacttgc atgcacgcga agttagcacg gccacgcggg agctatgcgc
     1321 tgcgatcaca caggaagcag cggctcacgc cctgttcgag ggggtagagc tcgggagctt
     1381 ccttgtcgca ccggtcgaag cgggcggggc agcgcggatg gaagcgacag ccgaacgggg
     1441 gattggcggg tgacggtact tcgccgcgta agagcgcacg ctcgactacc tcggggcgca
     1501 agcgggggac ggacgctagg agagcctcgc tgtaagggtg ctccggctcc tcgaagaacg
     1561 cctcggtggg ctgaacttcg acgatttggc ccagatacat caccgcgacg cgctgcgcga
     1621 cggcgcgtac gacgccgagg tcgtgcgtga tgaagaggta cgagaggccg agcgagcttt
     1681 gcagcccctt caagagctcg atgagctgcg cctgcaccga cacatcgagc gcggaggtga
     1741 cttcgtcgca gagcaagatg cgtggctcca ccgcgagagc gcgcgcaacg ctgatgcgct
     1801 ggcgttgacc gccagagaat tcgtgcggat agcgatcgcg gagcttcgga tcgagcccca
     1861 cttgcgagag gagctccgcc acgcgcgtat cgagctcgct cggcgtaagg aggcggtgca
     1921 gactcactgc ctccgcgagc gcgctgccga tgctgagacg ggggttcagg ctggcgtacg
     1981 gatcctgaaa cacgagttgc agctgttttc gcagggggcg cagcgcggcg cggccgagct
     2041 ttgcaatctc gcgttgctcg agcttcacgc tgccgccgct gctcggcacg agcccaacga
     2101 gagcgcgcgc gagcgtcgac ttcccgcaac ctgactcgcc gacgagcgcg agtgtttcgc
     2161 ccgggtaaag gtcgaggctc acgccgtcca cggcgcgaac caatccggcc gggctcggaa
     2221 agtgcacccg gaggtcacgc acgctgagca gcggctcggt catggggctc gctcgctctg
     2281 catttcttcg acgggggcga tgcaccttcg ccgccgcgtg gtcgcgaggg tgaacagctt
     2341 tggctcgccg tggcgacacc cttcacgaac tcgtgaacaa cgaggcgcga aggtacactc
     2401 ggtcaccggc tgaagcccga gccgtggcgg aaacccccca atgggcgaga gctttcctcc
     2461 gccagcttga tcgatgcgcg gcgtgctctt caagagcgct tcggtgtaag ggtgcagcgg
     2521 ttcgcggaac aagtcctggg tgagagcttc ttcgacgacc cggcccgcgt acatgacgac
     2581 cacccggtcc gcgagctccg cgacgacccc gaggtcgtgg gtgatgaaca aaagagacat
     2641 gttgcgctcg cgccggagct ctcgcaaaag ctcgagaatt tgcgcttgga tcgtaacgtc
     2701 gagcatcgtc gtcggctcgt ctgcgatgag cagatccgga ccgcacaaga gcgccatggc
     2761 gatgcagacg cgctgccgca tgccgcccga gagttggtgc ggatagccgc gcaggcgctt
     2821 ttcggcttcc gcgatgccga ctcgcgtgag gagctcgacc gcgcggctgc gcgcggcttt
     2881 tcgcgagagc ccgaggtgca cctcggcgac ttcgcacatt tgctcgtcga gggtgaggta
     2941 ggggttcaag ctcgtgagcg gatcttgaaa aacgagcgcg atgcgtcgcc cgcgcacgcg
     3001 gcggaggcgt tccgacgaga gcttcatcaa atcttccccg ccaaaccatg ccgaaccgcg
     3061 tgtgatgacg ccccgtccct ccaggagccc gagaacagcc aaggtgctta cgctctttcc
     3121 agagccggac tcacccacca acccgacgat ctcacctggc tcgatttcga agccgaggtc
     3181 gtccacggcg cgaacgcctt cctcgccgtt cgtgaactcg accgtgaggt tgcgcacgct
     3241 gagtaacgac atcagaagga gccctcgata ctcgttcgat tgacgacggg cggggcgagc
     3301 tttcgcatcc ttcggttcat cggcggatca ctcgcgagat ttggggtcga gcgcgtcgcg
     3361 aagaccgtcg cccaagaagt tcagactgag cagcgtgagg ccgaggaata ggctcggcca
     3421 gagcagaatc caccaaaagg agtgcaccgg gttcacgacc gcgacgccgt cggccacgag
     3481 ctgcccccaa gagagcgcga cgcccaaccc gaggaacgac aggaaagcct ccaggatgat
     3541 caccgacggc accatcaagg tggcgtaaac gatgacgacc ccgagcacat tgggcaggat
     3601 gtgtcggagc aagatgcgcg cgtcactcgc gccgagtaac tccgcggccg cgacgaactc
     3661 gcggtggcgc agcgaaatca cttggccgcg cacgatgcga gccatggtga gccactgaac
     3721 gagcccgagc gccaaaaaca ccggtagcgg gtcgccccgc gcgctctcgg cgaagagcag
     3781 catgatcagg atgacg
//
LOCUS       DASTKS010000813         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_798352, whole genome shotgun sequence.
ACCESSION   DASTKS010000813 DASTKS010000000
VERSION     DASTKS010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798352"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..2174)
                     /locus_tag="VFQ35_16805"
     CDS             complement(36..2174)
                     /locus_tag="VFQ35_16805"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET9932368.1"
                     /translation="MFSFKPKALSRLPPYIINGITVSLGLGIVQALAARVAHFAPAFP
                     GGSQFAIVGAICASLPHLPDRAGRAARRVLAGGVFGCVAEAVVLSLHGSRLGLGFAIP
                     ALVFCAMLMLSWGSRAGPIAFAGVLGAVFSLALPVAPDPLASAAWSLFGVLVYAAWSF
                     GSTLLLEARYRTLAVAACLRESARLLRARASIVTAPSVDGEPGALEAGLVAEEALLSS
                     LLQSARDLVFHAHDSAASRRVTAVLLRIMDLRDLALASRLDLEMLGTDDVARGTRQCL
                     ARGLVAVAEGLDAAEISLREGSAVAADDWAIDARRFADCALDGIAIADDDVRRPLLPV
                     ILNRLKHLADMVGRIHALLRAEDVVLSVPRDELRRFAQDEGWPRKVLLGNLSLESSVF
                     RHALRSALAVGSVFWLAHAMPWASRPHWLVLSVAVVLRGTVYETLSRRNARVLGTAIG
                     CVVAVALVRLVPDSVLAVVFLLAAGTAHAFVSARYLLTAIAATVMALLQIHFASPSIT
                     SSVVERLVDTSLGAGFAWAFSYVLPSWERRNLPRAVARALAALQDYAETALTPSDVIA
                     ESRLARQRAYDELDSLRSILPRIGAEPARVRPPVRELSAFIERAQILLAHLSSVRLIM
                     VRRRDQLDKESLELALGEARAALRALLAVRVQAPLSRPPQPSELDLPFEPPERQALPW
                     LTRRLKIALYDAAKVGAEARVTLEKLGATA"
     gene            complement(2421..>2697)
                     /locus_tag="VFQ35_16810"
     CDS             complement(2421..>2697)
                     /locus_tag="VFQ35_16810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241772.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9932369.1"
                     /translation="DVAKRLIDYGIHPPTVYFPLVVRGALMVEPTETESKQTLDAFIE
                     AMVQILAEAEREPELLLKAPHLTQLGRLDEARAARKPRLRWTRETPA"
BASE COUNT          525 a          899 c          906 g          367 t
ORIGIN      
        1 ctgccgccat ccatcgaggg ccgcaagtgt ggcaactacg cagtcgcccc gagtttctcc
       61 agcgtaacac gcgcttctgc gccgactttc gccgcgtcat agagcgcgat tttgagtcgt
      121 cgggtcagcc acggcaaagc ttgccgctcg ggcggctcga agggcaagtc gagctccgag
      181 ggctgtggcg gtcgggagag gggcgcttgg actcggaccg cgagcaacgc gcgcaaagca
      241 gctcgggcct cgccgagcgc gagctcgaga gactccttgt cgagctggtc gcgtcttcgg
      301 accatgatga gccgaaccga tgaaaggtgg gctagcaata tttgcgcgcg ttcgatgaac
      361 gcgctgagct cgcgcaccgg tggccggacg cgcgccggct cggcgccgat gcgtggaagg
      421 atcgagcgca gcgagtcgag ctcgtcgtaa gcgcgttgtc gtgcgagccg cgactccgca
      481 atcacgtccg acggagtcaa cgccgtctcg gcgtagtcct gcaacgccgc caaggcgcga
      541 gccaccgcgc ggggcaaatt tcgccgctcc caggatggca gcacgtagct gaacgcccaa
      601 gcgaacccag cgcccaacga cgtgtccacg agccgctcga ccacggagct cgtgatgctc
      661 ggtgacgcga agtgaatttg caagagcgcc atcacggtcg cggcaatcgc ggtgagcaga
      721 tagcgcgcgc tcacgaatgc atgcgccgtc cccgctgcca gcaagaagac cacggccaga
      781 acggagtccg gaaccaaacg aaccagggcc accgcgacga cgcatccgat ggcggtgccg
      841 agaacacgcg cgttgcgacg cgacaaggtc tcgtagacgg tgccgcggag caccaccgca
      901 acgctcaaca cgagccagtg cggccgcgac gcccacggca tcgcgtgagc gagccagaag
      961 acgctgccca ccgcgagggc gctccgcagc gcgtgccgga acacggaaga ctccaagctc
     1021 aggttgccga gaagaacctt gcgcggccag ccttcgtctt gggcaaagcg gcgcaactcg
     1081 tcgcgcggaa cgctcaggac cacgtcttcg gctcgaagca gcgcgtgaat acggccgacc
     1141 atatccgcga ggtgcttcaa gcggttcaga atgactggta agagaggccg ccggacgtcg
     1201 tcgtccgcga tcgcgatgcc gtcgagcgcg cagtcggcga accgcctcgc gtcgatggcc
     1261 caatcgtccg ctgcgaccgc agagccttcg cgcagcgaaa tctctgccgc gtcgagccct
     1321 tcggccaccg cgaccaagcc gcgcgcgaga cactggcgcg tgccgcgcgc gacatcgtcc
     1381 gtgccgagca tctcgagatc gaggcggctc gccagcgcaa ggtcccgcaa atccatgatc
     1441 ctgaggagga ccgcggtgac gcgccggctc gcggcgctgt cgtgagcgtg aaagacgagg
     1501 tcgcgtgcgg actgcagcag cgacgagagc aacgcttctt ccgcaaccaa gccagcttct
     1561 agcgccccgg gctcgccgtc cacggagggc gccgtcacga tgctggctcg agctcgcaag
     1621 aggcgcgcgc tctcacgtaa gcacgcggcc acggcaaggg tgcgatagcg agcctcgagc
     1681 aacagagtgc tgccgaagct ccacgccgcg tacaccaata cgccgaacag gctccacgcc
     1741 gcgctcgcga gcgggtccgg ggcaaccggc aacgccaagg agaacacggc gccgagcacg
     1801 cccgcaaacg cgatgggccc cgcccgcgaa ccccacgaga gcatgagcat tgcgcagaaa
     1861 accaacgccg gaatggcgaa accgagcccc aaacgcgagc cgtgcaacga cagcaccacg
     1921 gcttccgcta cgcagccgaa gacgccgccc gcgaggactc gtcgcgcggc tctgccagcg
     1981 cgatccggca ggtgcggaag gctcgcgcaa atggcgccga cgatcgcaaa ctgagaaccg
     2041 ccgggaaacg ccggcgcgaa gtgcgcgacc cgcgcggcca gcgcttgaac gatgcctagc
     2101 ccgagcgaca ccgtgatgcc gttgatgatg tacggtggaa gccgtgacag cgccttcggc
     2161 ttgaacgaaa acatgtccgc gagaaggagg aagagcgggt ggacgaactc cactgtacag
     2221 gccgggtgcc aacctggggg cctgattttc cttgcctcgg cgcgcgctct ctctcggatt
     2281 tgcggctgag aggcgatcgg gcgcgcacta cgtcgtcaca tgaggcgtat tgcgcgcccg
     2341 agcagcgcga tcacggggct cgacgaaatc cgcctcgcta actcgcgtgc ctgctcgagt
     2401 ggagcgacac tgataccagc ttacgcgggt gtctcgcgcg tccaacgcag gcgaggcttg
     2461 cgtgccgcgc gcgcttcatc gagacggccg agctgggtaa ggtggggggc cttcaagagg
     2521 agctctggct cgcgctcggc ctcggccagg atttgaacca tggcttcgat gaaggcgtcg
     2581 agagtctgct tcgactccgt ctcggtgggt tcgaccatga gcgcgccacg cacgaccaag
     2641 gggaagtaca ccgtcggcgg gtgaatcccg tagtcgatga gccgcttggc gacgtcg
//
LOCUS       DASTKS010000814         7529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1021672, whole genome shotgun sequence.
ACCESSION   DASTKS010000814 DASTKS010000000
VERSION     DASTKS010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021672"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFQ35_16815"
     CDS             complement(<1..301)
                     /locus_tag="VFQ35_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932370.1"
                     /translation="MKRAHLLFAAPLLLAACESCRRERPYTPFGVTSALPTTASPVDA
                     AAPSASAPPLLPGPDTSIKKAVMAPADAHRWNLEGRDLDAPPGRHFEQALVADFNA"
     gene            complement(309..1106)
                     /locus_tag="VFQ35_16820"
     CDS             complement(309..1106)
                     /locus_tag="VFQ35_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9932371.1"
                     /translation="MKANSATTRLEVHGGVATITLNRPDKLNALSGAVLDELFAVVSE
                     LDARAPSERPRVLVIASASDRAFAAGADISELGGLDAEAARELSERGHRLGRALDSAS
                     FPSIAAVNGFALGGGLELALSCDLIVASDKARFGQPEINLGLIPGFGGTFRLAARVGT
                     GRARRLIYTGETIDAARAEVMGLVDAVFAHAEFSARVNELAHALASKAPLALGTAKQC
                     LLANQYQSYASSAALEAKRFGALFSTADAREGIEAFLTKRPASFTGR"
     gene            complement(1103..2035)
                     /locus_tag="VFQ35_16825"
     CDS             complement(1103..2035)
                     /locus_tag="VFQ35_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9932372.1"
                     /translation="MNAEGLSGPSAATGQRPDWPTRAAVIGAGQMGGGIAQVLCLAGI
                     DVAVCDVSRERAAAAVRSVLGRLERLTQKGKLDAARLETARQKLLPHEFDEALADAEL
                     VIEAASEDPALKTQLFKRADAAAPAAAVLASNTSSISITRLGAVTSRPDRVVGMHFMN
                     PVPLMKLVEVVRGIATSPATVDLILGLCQRLEKTAIQSSDSPGFLVNRMLLPFLNEAC
                     FALQEGVGTAEDIDQGARLGLNHPLGPLELADLIGLDTVLAIAEVLHRDLGDDKYRPP
                     TLLRNLVAAGYLGRKSGRGFYSYGADGQKRGTDS"
     gene            2203..2961
                     /locus_tag="VFQ35_16830"
     CDS             2203..2961
                     /locus_tag="VFQ35_16830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932373.1"
                     /translation="MLLADKGQNREAVALLEGELTKHPRLVRERRLLIRLYGSLGDLG
                     KARNEANVLAKQLGPQSPLPWLELGHAHELCHRYDVALELYDYASAVAPLDPAGPKTG
                     GIRAAAWGELEAAEPRLSEAVRRDVRDARSFHALGLVRTKLGDLDGAERAYRAGVLSD
                     PRGIDNRIGLATLALVRGDVDRVLAEYEAILQLYPKFADAELGRSWALGKLGRLTEAR
                     AALERAARLGASPKSVERQRAWLADERAKSATTR"
     gene            3074..>7529
                     /locus_tag="VFQ35_16835"
     CDS             3074..>7529
                     /locus_tag="VFQ35_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932374.1"
                     /translation="MDSREIRTALGKLQAEPDAEQAWQALTAAAKDTSGDLSSADFIQ
                     LLEAASEEHKKRGEWPAVARLLELAVTAARDTEREPELLAAHAKVLSEELFDDDAAAV
                     VYLRLLERRPQDPVAVAAIEESDGRRRRMTELHESYLTEVERATDDVYKSSMLMRASE
                     LEVRFAAPSTDLESAIDRLEQAVRLDPTNVRAGRLLEHVYRRRGAWEELARVLERMAD
                     RSERASERVPAGVRLARVYAQHLSDRERAARAYDRVLRDDASHAEAKAFLSELYSSEE
                     RWAELVALYERELKSKDLHDAERLGDMLQIAMLHWKKLEHPQDAEPWFERVRKVDPTN
                     EGMLTFYREYCRTLEDDTRLMDVLQGAQRALKDGTKEKTRLAQEIARLAEGQANAAKA
                     IEQYKSVLRQDPDNAEARERLKTLYKQTQGHNALVELLRQQLERTPQDKYAERLAILR
                     EVATVYRQYQKSDTALLSVLNQIVQLDDKLDEHDVLELREIVALYDKLGRHRELITHQ
                     IKLAEVTPDVEEKRELYRAAARRWLDQFSNVQNATEAYAKLLAIAPEDREARERLEEL
                     YRKRRAWSELFALYSSELERAEGQQRLTLLREMAQLAAERLNRSEEAVRLYKQILEAE
                     PERADVLDALEKHAERNKDFRTLAEALERRVNLVTDDAAKLAALQKLGAIYAEQLADP
                     AAAAHTWRRVLTLSPGHNRALRVLRETYLASGDYDGLEQLYGSQNDWEGLAEVLSNAA
                     DRAKEATTRVELSYRTARVLAEHLNQPERAFRSYERILAADPTDTRAARALVPLYERD
                     EKWSRLPALYELLLEKAESSDEKLALFEKLVDVSGKRLSDRRAAAAYARRAYELAPTH
                     PRALSLFEEASRAASSWDVFVEVIGARLAAEPVVAAQANEASSPTASGDAKKKGKKKK
                     KGAEAAAADAPVSNVTNEIPSDPVGDRRAVELMLARVYAEELGRSDDAVAIYKRLLER
                     DPLDPDARTRLEAILRREDRRDDLRWLLELKIDATSDPSEKLDMLAEFAELEEDVFMA
                     PERAVALYRRILDLGPSDERALRALPRLLLAADDVAGAVQIIERHRDVLGGNARGERE
                     ADLAELYADRLGKPEAALESAIAALASPPHVARAIAVLETLLRVDGVRARAAEVLADR
                     YAESGDARKEVAALEVMLACAKDPSERMSLYQRLADAQEKKLSSYGNALDTMLSAVRE
                     FPKELELWTRADLLSGLAGRPTDLADAYREVLRRDLPKELEIELSERAAHLHEDKLGD
                     PMGAIPYLESVLALAPSNEAAFQRLKDILTGAERWAELEALYDRASNATADVERRIEM
                     LVEVALICEEIIEDPAKATRYYERILETDPLHDGAVRALDRLYVRQGKDRELANLLDK
                     RLETASGEEALELKLRLAKLQLDLHEPNKAVAHVEDVLQERVQNYEARSLAERMLEIG
                     QLRVRAARMLENVYEARDEMRELVRVLQIRLDGLDNSPEAGLEQRDLLR"
BASE COUNT         1304 a         2536 c         2567 g         1122 t
ORIGIN      
        1 ccgcgttgaa gtccgcgacc agcgcttgct cgaagtgacg ccccggtggg gcgtcgagat
       61 cgcgcccctc gaggttccag cgatgcgcgt cggcgggagc catgaccgcc ttcttgatgc
      121 tcgtgtccgg ccccgggagg agcggcggag cgctcgcgct gggcgcggct gcgtccacgg
      181 gcgaagcggt ggtcggcagc gccgatgtca ccccgaaggg tgtgtacggt cgctcgcgtc
      241 ggcagctctc gcacgccgcc aagaggagcg gcgctgcgaa aaggaggtga gctcgcttca
      301 tcttcgaatc agcgtccggt gaagctcgct gggcgtttgg tgaggaacgc ctcgatgccc
      361 tcacgggcgt ccgccgtcga aaacagcgcc ccgaagcgct tggcttcgag agcggcggag
      421 cttgcgtagg actggtactg attggcgagc aggcactgtt tggcggtgcc gagcgcgagc
      481 ggcgccttgc tcgcgagcgc gtgcgcgagc tcgttcacgc gcgccgaaaa ctcggcgtgg
      541 gcgaagacgg cgtcgacgag acccatcacc tcggctcgcg cggcgtcgat ggtctcgccg
      601 gtatagatga gccgccgggc gcggcccgtg ccgacgcgcg ctgccaaccg aaaggtgccg
      661 ccgaaccccg ggatcaagcc caggtttatc tccggttggc cgaagcgcgc cttgtcggag
      721 gcgacgatga gatcacagct gagcgcgagc tcgagcccgc cgccgagcgc gaagccgttg
      781 acggccgcga tgctgggaaa gctcgccgag tcgagcgcgc gccccaagcg atgcccccgc
      841 tcactgagct cgcgcgccgc ttccgcgtcg agcccgccga gctcgctgat gtcggcgccc
      901 gcggcaaagg cgcgatcgct cgcggaggcg atgaccagca ctcggggacg ctcggaagga
      961 gctcgcgcgt cgagctcgga cacgactgcg aagagctcgt cgagcaccgc gccgctcagc
     1021 gcgttgagtt tgtcgggacg gttgagcgtg atggtggcaa cgccgccgtg cacttcgagg
     1081 cgggtcgtcg cggagttggc cttcatgagt cggtccctct cttttgtccg tccgcgccgt
     1141 aactatagaa accacgcccg gacttcctac ccagatagcc tgccgcgacc aggttgcgaa
     1201 gcaacgtcgg agggcggtac ttgtcgtccc ccaagtcgcg gtggagcacc tctgcgatgg
     1261 cgagtacggt gtcgagcccg atgagatcgg cgagctcgag ggggccgagc ggatgattga
     1321 ggccgagccg cgcgccttga tcgatgtcct cggccgtccc cactccttct tgaagcgcga
     1381 agcacgcctc gttgaggaag gggagcagca tgcggttcac caagaatccc gggctgtcgg
     1441 agctctggat cgcggtcttt tcgagccgtt ggcagagtcc caagatgagg tcgacggtgg
     1501 ccgggctggt cgcgatgccg cgcaccacct cgacgagctt catcaaaggg accgggttca
     1561 tgaagtgcat gccgaccacg cggtcgggac gcgacgtaac cgcacctagt cgcgtgatgg
     1621 agatgctgga cgtgttcgag gcgagcaccg ccgcagccgg cgccgccgca tcggcacgct
     1681 tgaagagctg cgtcttcaac gcggggtctt cgctcgcggc ctcgatgacg agttcggcgt
     1741 cggcgagcgc ctcgtcgaac tcgtggggca aaagcttctg ccttgcggtc tcgagccgcg
     1801 cggcatcgag cttgcccttc tgggtcagtc gttcgagccg gcccagcacc gagcgaaccg
     1861 ccgcggcagc tcgctcgcga ctgacgtcgc agaccgcgac gtcgatgccg gctaaacaaa
     1921 ggacctgggc gattcccccg cccatttgcc cggccccgat gaccgcggcg cgcgtcggcc
     1981 aatccggccg ctgcccagtg gccgcgcttg gaccactcag gccctcggcg ttcattgccg
     2041 actcgtaaca atgagcacgg ggcttcgcaa gtctcgtggt atcgtgcagc gcgtcggatg
     2101 cgccgagggt tttcgcccaa cctcctttat tgggcaggcc tcctcgcact gggggtggcg
     2161 attgccggat gcgctcgcgg ctcccacgtg agcgagcgag cggtgcttct cgctgacaag
     2221 ggacagaacc gcgaggcggt tgctctgctc gaaggggagc tcacgaaaca tccgaggctc
     2281 gttcgcgagc ggcgcttact cattcggctg tatgggtcgc tcggggacct cggcaaggcg
     2341 cgcaacgaag cgaatgtgct ggcgaagcag ctcggcccgc aaagccctct gccatggctc
     2401 gagctcggtc acgcgcatga gctctgtcat cgctacgacg tcgcactcga gctctacgac
     2461 tacgcgtcgg cggttgcgcc gctcgatccg gccggcccga aaaccggtgg catccgcgcg
     2521 gctgcgtggg gagagctcga agccgccgag ccgcgcctca gcgaagcggt gcggcgcgat
     2581 gtgcgcgacg ctcgctcctt tcatgcattg gggctcgtgc gtacgaagct cggagacctc
     2641 gacggagcgg aacgcgcata ccgagcgggc gttctctctg acccgcgcgg catcgacaat
     2701 cgcatcggac ttgcgacgct cgcgctcgtg cgcggtgacg tcgatcgcgt gctcgccgag
     2761 tacgaagcaa ttctgcagct ttacccgaaa ttcgccgacg ccgagctcgg tcgctcgtgg
     2821 gcgctcggca aactcggtcg gttgaccgaa gcacgcgcgg cgctcgagcg agcggcgcgt
     2881 ctcggcgcgt ctccgaagag cgtcgaacgg cagcgcgcat ggctcgcgga tgagcgagcg
     2941 aagtctgcga caacccgctg aaaatctagg ccgtagctgg gcttgtaagc cccagtcgag
     3001 cggcgtaacg tgagccccca cccaagagcg cgtggtccag cgctatcacg gggttggttg
     3061 cacgggttga ggaatggatt cccgagagat ccgcacggca cttggaaaac ttcaggcaga
     3121 gccggatgcc gaacaggcat ggcaggcgct aaccgcagca gcgaaagaca cgtcggggga
     3181 cctgagctcc gccgacttca ttcagctgct cgaggccgcg agcgaggagc acaaaaagcg
     3241 aggagagtgg cccgccgtcg cgcgactact cgagctcgcg gtgacggccg cgcgcgacac
     3301 tgagcgcgag cccgagctgc tcgccgcgca cgccaaagtg ctgagcgaag agctcttcga
     3361 cgacgatgca gctgccgtcg tttacctgcg tttgctcgag cggcgtccgc aagatccggt
     3421 tgccgtcgcc gcgattgaag agagcgacgg ccgtcgtcgg cgcatgaccg agctccacga
     3481 gagctacctc accgaggtcg agcgcgcgac ggacgacgtt tacaagagct cgatgctgat
     3541 gcgcgcttcg gagctcgagg tgcgcttcgc cgccccgagc acggatctcg agtctgccat
     3601 cgaccgcttg gagcaagcgg ttcgtctcga ccccaccaac gtgcgcgccg gcaggttgct
     3661 cgagcacgtg tatcgtcgac gcggcgcgtg ggaagagctc gcccgcgtcc tcgagcgcat
     3721 ggcggaccgc agcgagcgcg cgagcgaacg cgtgccggcg ggagtacgcc ttgcccgcgt
     3781 ctacgctcag catctgagcg accgcgagcg cgccgcgcgg gcttacgatc gcgtgctgcg
     3841 cgacgacgcg agccacgccg aagcgaaggc gttcttgtcg gagctctact cgagcgaaga
     3901 gcgctgggcg gagctcgtgg cgctctacga gcgcgagctg aagagcaagg acttgcacga
     3961 cgccgagcgc ctcggcgaca tgctgcaaat cgcgatgctg cactggaaga agctcgagca
     4021 tccgcaagac gcggagcctt ggttcgagcg cgtgcgcaag gtcgacccca cgaacgaggg
     4081 catgctcacc ttttaccgtg agtactgccg cacgctcgag gacgacacgc gtctcatgga
     4141 tgtgctccag ggcgcgcagc gcgcgttgaa ggacggcacc aaggagaaga ctcggctcgc
     4201 gcaggagatc gcgcggctcg ccgaagggca ggccaacgcc gccaaggcga tcgagcaata
     4261 caagagcgtc ctccggcaag atccggacaa cgccgaggcg cgcgagcgcc tgaagacgct
     4321 ctacaagcag actcaggggc acaacgcgct cgtcgagctc ttgcgccagc agctcgagcg
     4381 cacgccgcag gacaagtacg ccgagcgact cgcgatcttg cgcgaagtcg cgaccgtgta
     4441 tcgccagtat cagaagagcg acaccgcgct gctctcggtg ctgaaccaga tcgtgcagct
     4501 cgacgacaag ctcgacgagc acgacgtgct cgagctccgc gagatcgtcg ccctatacga
     4561 caagctcggc cgacaccgcg agctcatcac gcaccaaatc aagctcgccg aggtcacgcc
     4621 cgacgtcgaa gagaagcgcg agctctaccg agcggccgcg cgccgctggc tcgaccagtt
     4681 ctccaacgtg cagaacgcga ccgaagcgta cgcgaagcta ctcgcgatcg cgcccgagga
     4741 ccgcgaggcg cgcgagcgct tggaggagct ctaccgcaag cgccgcgcct ggagcgagct
     4801 cttcgctttg tactcttcgg agctcgagcg cgccgaaggt cagcagcggc tcacgctgct
     4861 gcgcgagatg gcccagctcg ccgcggagcg cctgaaccgc tccgaagaag cggtccgcct
     4921 ctacaaacaa atcctcgaag ccgagcccga gcgcgccgac gtgctcgacg cgctcgagaa
     4981 gcacgccgag cgaaacaaag attttcgcac cttggccgag gcgctcgagc gccgcgtgaa
     5041 cctcgtcacc gacgatgcgg cgaagctcgc ggcgctgcaa aagctcggcg ccatttacgc
     5101 cgagcagctc gcggatcccg ccgcggccgc gcacacgtgg cggcgcgtgc tcacgctctc
     5161 gcccgggcac aaccgcgcgc tgcgcgtact tcgcgagacc tacctcgcga gcggcgacta
     5221 cgacggtctc gagcaactct atggctcgca gaacgactgg gaaggcttgg ccgaggtgct
     5281 gagcaacgcc gccgatcgcg cgaaagaagc gacgactcgc gtcgagctca gctatcggac
     5341 ggcccgcgtg ctcgccgagc acttgaacca gccggagcgc gccttccgct cctacgagcg
     5401 catcctcgcc gcggatccga ccgacacccg cgccgcgcgc gcgctcgtgc cgctctacga
     5461 acgcgacgag aagtggtcgc gcctgccggc gctgtacgag ctcctactcg agaaggcgga
     5521 gtcctccgac gagaagctcg ccctgttcga gaagctcgtc gacgtcagcg gcaagcgcct
     5581 ctcggatcgt cgggccgcgg cggcgtatgc acgccgcgct tacgagcttg cgccgacgca
     5641 cccgcgtgct ctttctttgt tcgaagaggc gtcgcgcgcg gcaagctcct gggacgtatt
     5701 cgtggaggtg attggagcgc gcctcgcggc cgagcccgtc gtggctgcgc aagcgaacga
     5761 ggcctcgtct ccgaccgcca gcggcgacgc caagaagaag ggcaagaaga agaaaaaggg
     5821 agcggaagcc gcggccgcgg acgcccccgt ctccaacgtc acgaacgaga tcccgtcgga
     5881 tccggtcggt gatcgccgcg cggtcgaact gatgctcgcg cgggtgtacg ccgaagagct
     5941 cggccgtagc gacgacgcgg tcgcgatcta caagcgcctg ctcgagcgcg atccgctcga
     6001 ccccgacgcc cgcacgcgtc tcgaggcgat tttgcggcga gaagaccgcc gcgacgacct
     6061 tcgttggctc ttggagctca agatcgacgc gaccagcgac cccagcgaga agctcgacat
     6121 gctcgccgag ttcgcggagc tcgaggaaga cgtcttcatg gcgcccgagc gtgccgtcgc
     6181 gctctatcgt cgcattttgg acttgggtcc gagcgacgaa cgcgcgctgc gggcgctgcc
     6241 gcgcctgttg ctcgccgcgg acgacgtcgc gggcgccgtc caaatcatcg agcgccatcg
     6301 cgacgtgctc ggcggcaacg cgcgcggcga gcgcgaggcg gatctcgcag agctctacgc
     6361 ggatcgcttg ggcaagccgg aggccgcgct cgaaagcgcc atcgccgcgc tcgcttcgcc
     6421 tcctcacgtg gcccgtgcca tcgccgtgct cgagacgctg ctgcgggtcg acggcgtgcg
     6481 cgcgcgcgcg gccgaggttc tcgcggatcg ctacgccgag tcgggagacg ctcgcaaaga
     6541 ggttgccgcg ctcgaggtca tgctcgcttg cgcgaaagat ccgagcgagc gcatgagcct
     6601 ctaccagcgc ctcgccgacg ctcaggagaa gaagctctcg agctacggca acgcgctcga
     6661 cacgatgctc tccgccgtgc gcgagttccc gaaggagctc gaactctgga ctcgcgccga
     6721 cctgctctcc ggcttggcgg gccgccccac ggatctcgcg gacgcttacc gagaggtgtt
     6781 gcgtcgcgac ctgccgaagg agctcgaaat cgagctctcg gagcgcgccg ctcacctgca
     6841 cgaggacaag ctcggggacc cgatgggcgc gattccctac ctcgagagcg tgctcgccct
     6901 cgcgccgagc aacgaggccg cgttccagcg cctgaaggac atcctcaccg gcgcggagcg
     6961 ctgggccgag ctcgaggcgc tctacgatcg cgcctcgaac gccaccgccg acgtcgagcg
     7021 tcgcatcgag atgctcgtcg aggtggccct catttgtgag gagatcatcg aggatccggc
     7081 gaaggccacg cgctactacg agcgcatcct cgagacggac ccgttgcacg acggcgcggt
     7141 gcgcgcgctc gatcggctct acgttcgcca gggcaaggat cgcgagctcg ccaacctgct
     7201 cgacaagcgc ctcgagaccg cgagcggcga agaagcgctc gagctcaagc tgcgcctcgc
     7261 gaagctgcag ctcgacctcc acgagccgaa caaggcggtc gcgcacgtcg aggacgtgct
     7321 gcaagagcgc gtgcagaact acgaggcgcg gagcctcgcc gagcgcatgc tcgaaatcgg
     7381 tcagctgcgt gtgcgcgccg cgcgcatgct cgagaacgtg tacgaggcgc gcgacgagat
     7441 gcgcgagctc gtgcgggtcc ttcagatccg cctcgacggc ctcgacaaca gccccgaggc
     7501 cggtctcgag cagcgcgacc tcttgcgcc
//
LOCUS       DASTKS010000815         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1246444, whole genome shotgun sequence.
ACCESSION   DASTKS010000815 DASTKS010000000
VERSION     DASTKS010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246444"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..556)
                     /locus_tag="VFQ35_16840"
     CDS             complement(299..556)
                     /locus_tag="VFQ35_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932375.1"
                     /translation="MELGSGVALGFVFATLLGPRFLSWWYEPPSKLAISCGPSVESAL
                     SQFVVVQLVSAALGGIFVSLILFYIRRLWRRRAQVEPTPKP"
     gene            complement(799..1491)
                     /gene="urtE"
                     /locus_tag="VFQ35_16845"
     CDS             complement(799..1491)
                     /gene="urtE"
                     /locus_tag="VFQ35_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HET9932376.1"
                     /translation="MLEVSELNQYYGESHTLWDMSLSVPEGSCTCLMGRNGVGKTTLL
                     KTVMGLLPARSGKIRLGAHELAGSPAETRARRGIGYVPQGRDIFGQLTVAENLRTGLG
                     ARSDGKKEVPERIFELFPVLKSMMRRRGGDLSGGQQQQLAMGRALALDPKLLILDEPT
                     EGIQPNIVSQIADVLNQLRKAGLTILLVEQKLPFARRVADRFCIMDRGRRVAAGEMSE
                     LSDEVVSKYLTV"
     gene            complement(1491..2411)
                     /gene="urtD"
                     /locus_tag="VFQ35_16850"
     CDS             complement(1491..2411)
                     /gene="urtD"
                     /locus_tag="VFQ35_16850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HET9932377.1"
                     /translation="MSGKALVLNETESVPPDADESGKSPVSTPVPKSPRRAPISLFPR
                     RRTVDKSRVDALTKGRKALLCVDDISVSFDGFKALNNLTLVLDEGELRCIIGPNGAGK
                     TTLQDVITGKTKPDSGSVFLVGDDTDLTHLSEHEIAQRGVCRKFQRPTVFQGHSVFEN
                     LELALPGKKSVFQSLFARLSAKQKEKIESILETIGLTDQMFTRAGLLSHGQKQWLEIG
                     MLLAQDPRVLLVDEPVAGMTHQETERTAELLNSLAGERTVVVVEHDMEFVRSIARTVT
                     VLHEGSVLAEGPMDVVQNDPKVIEVYLGES"
     gene            complement(2408..>2862)
                     /locus_tag="VFQ35_16855"
     CDS             complement(2408..>2862)
                     /locus_tag="VFQ35_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET9932378.1"
                     /translation="AGLMAAFLICRFVTTSKAGRVLRSIRDEESRIMFLGYDPTRFKL
                     FVYTLSAVLCGFAGALYVPQVGIINPGELQPSNSIEMAIWVAVGGRGSLLGAPLGAFV
                     VNGAKSWFTVALPDLWLYVLGALFVAVTLFFPRGLVGALEGALVRVRK"
BASE COUNT          528 a          894 c          931 g          509 t
ORIGIN      
        1 gaatgaacca aagcccgagg gcgggagctg ggatcgccgg gcaaaaggtt ttgacgttgt
       61 gacatatttt gtcaaaacgt tcacgcagca taaagaggcc gtccgcttgg aaatggcgtc
      121 acgccgacta gggtcgccgg gtgtgcgaca gcgcgggact agctgcgggt gtgcgacagc
      181 gcgggactgc cgcgggcgtg cgacagcgcg gtctgctgcg ggcggtgcga cagagagcag
      241 gactgctgcg ggggtgcgac agcgcgggac tactgtggct cttgctcgac gctagcgctc
      301 aaggcttcgg tgtgggttcc acttgcgcgc ggcggcgcca aagccttcgg atgtaaaaca
      361 agatcaagga gacgaagatc ccgccgagcg cggccgacac gagctgcacg acgacgaatt
      421 ggctgagcgc gctctccacg ctgggcccac acgatatggc gagcttgctg ggcggctcgt
      481 accaccagct cagaaagcga ggccccaaca acgtcgcgaa aacgaagcca agtgcaacgc
      541 cagacccgag ctccatggca aagcgtttca gtggaagagg atttccttca gccatagcgc
      601 gagcaggcta ccccagccct tcgcgaagcg cactctgtgt gcaggccttt ccacgatagg
      661 cttcggaatg cgcaacaggc gctgctcggg gtctcggtgc gtcccgacaa agcggtcacg
      721 ggcgagcgcg gggcggtaat gtcttcgcgg tcggcgttgg ccctacgacg agccgccgca
      781 cggcgaccac tgtctggctc agaccgtgag gtacttgctc accacctcgt cgctgagctc
      841 ggacatttcg ccggcggcca cgcgacggcc tcggtccatg atgcagaagc ggtcagcgac
      901 ccgccgcgcg aacggcagct tctgctcgac gagcaagatg gtaagtcccg ccttgcggag
      961 ctgattcaag acgtcggcga tttgcgaaac gatgttcggc tgaatgccct cggtcggctc
     1021 atccaagatc aagagcttgg gatcgagcgc gagtgcgcgg cccattgcga gttgctgctg
     1081 ctggccgccg gagagatcgc cgccccgccg acgcatcatg ctcttcaaca cgggaaagag
     1141 ctcgaagatg cgctcgggga cctccttctt tccgtcgctg cgcgcgccga ggccagtgcg
     1201 gaggttctca gcaacggtga gctgcccaaa gatgtcgcgc ccctgtggta cgtagccgat
     1261 accgcgccgc gcgcgcgttt ccgcgggcga cccggcgagc tcgtgtgcgc caaggcgaat
     1321 cttgccggag cgcgcgggca agaggcccat gaccgtcttg agcagcgtcg ttttgccgac
     1381 cccgttgcgc cccatcagac aggtgcagga gccctcaggc acgctgagcg acatgtccca
     1441 aagggtgtga ctttcgccgt aatactgatt gagctcgcta acttccagca tcagctttcc
     1501 ccgagataga cttcgatgac cttcgggtcg ttctgcacga cgtccatggg accttcggcg
     1561 agcacgctgc cttcgtggag cacggtcacg gtgcgcgcaa tgctgcgcac gaactccatg
     1621 tcgtgttcga cgacgacgac ggtgcgctcg ccggcgagcg agttcaggag ctctgccgtt
     1681 cgctccgttt cttgatgggt catccccgcc accggctcgt cgacgagcag cacgcgcgga
     1741 tcctgtgcga gcagcatacc gatctcgagc cattgctttt gaccatgcga gaggagccca
     1801 gcccgcgtga acatttggtc ggttaggccg atcgtctcca aaatgctctc gatcttctct
     1861 ttctgtttgg ccgaaaggcg ggcgaacagg ctctgaaaga cgctcttctt cccgggcagc
     1921 gcgagctcga gattttcgaa tacgctgtgc ccctggaaga cggtcgggcg ctgaaatttg
     1981 cggcacacgc cgcgctgggc gatttcgtgc tcgctcaagt gggtgaggtc cgtgtcgtct
     2041 ccgaccaaaa agacgctacc gctgtcgggc ttggtcttgc ctgtgatcac gtcctgaagc
     2101 gtagtcttgc cggcgccgtt cggtccgatg atgcaacgga gctcgccttc gtcgagcacg
     2161 agcgtgaggt tgttcaacgc cttgaacccg tcgaagctca cgctgatgtc gtcgacgcag
     2221 agcagcgcct tccgtccctt cgtcagcgcg tcgacgcgcg acttgtcgac ggtgcgacgc
     2281 cgcggaaaga ggctgattgg cgcccgccgc ggtgacttcg gcaccggcgt gctgacaggg
     2341 gactttccag actcgtctgc gtccggaggg acgctctcgg tttcgttcaa gaccagtgcc
     2401 ttcccgctca ttttcgcacc cgtacgaggg cgccttcgag cgcgccgacc aaaccacgcg
     2461 gaaaaaagag tgtgacggcg acgaacagcg cgccgagcac gtagagccat agatccggca
     2521 gcgcgaccgt gaaccagctc ttcgcgccgt tcaccacgaa cgcgccgagg ggcgcgccga
     2581 gcaggctgcc gcgcccgccg accgcgaccc agatcgccat ttcgatcgag ttcgagggct
     2641 ggagctcacc cgggttgatg atgccgacct gcgggacgta gagtgcacca gcaaagccgc
     2701 acagcactgc ggagagggtg tagacgaaca gcttgaagcg cgtcgggtcg tagccgagga
     2761 acatgatgcg gctctcttcg tcgcggatcg agcgcagtac gcggcccgct ttcgaggtcg
     2821 tgacgaagcg gcagatcaga aaagccgcca tcaagcccgc cg
//
LOCUS       DASTKS010000816         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_353059, whole genome shotgun sequence.
ACCESSION   DASTKS010000816 DASTKS010000000
VERSION     DASTKS010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353059"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFQ35_16860"
     CDS             complement(<1..601)
                     /locus_tag="VFQ35_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932379.1"
                     /translation="MKYSSPIAHGAGHRQRRCAPLERMPLWLLVALALVAVLACSPAD
                     LQAITESSGGSSGADTTGSGGAPPSGGASHGGGGAAPSGGTRSSGGAPNASGGSGGAA
                     CPATSLRPGDTVETLRIGGKTRSFVLHVPSTYDGSRRVPLVVDFHGMGSSGSSELANS
                     AFTALTDPESVIWAFPDGLKGPLGTAWNLGPCCVADVDDL"
     gene            complement(598..1635)
                     /locus_tag="VFQ35_16865"
     CDS             complement(598..1635)
                     /locus_tag="VFQ35_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932380.1"
                     /translation="MNRLAIAALCSVVWLWATTALAQKVVLLRPPRPSPSVAEVLARL
                     HGELLSIGIEVSFTAASPASSSSAESEAIRELARDSEAEAVLEVLGDVNPQAIHVWIA
                     DSKRDYEPALVELSTDQNAPNELAIRAVEVLRSRLLEGDLTPRRSVSPPKHAAATPGT
                     LRPKNPVPPREDERNRRLGFEIGAATLISFDRLGPAVLPLARVNWSLDAPVVLQATAA
                     GLGTRPNVATNAGSAQVAQQYVLVGARAGFREKRSLRPFFALSAGALRTAVEGVASGA
                     GEGHSLSQWSFLVDAGVGASLELGAHYSLSLVTEVQLAQPYVVIHFLDDAVASSGRPN
                     LVVALAFGAWP"
     gene            complement(1643..2947)
                     /locus_tag="VFQ35_16870"
     CDS             complement(1643..2947)
                     /locus_tag="VFQ35_16870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9932381.1"
                     /translation="MTPAPEREGRETRGLAALTRLGRDAVERPSAAELDKGLNVLLAR
                     IAAREAGRTHWVRRSLVAVAALACLVGGIRLSGALYQRWTSHPPALSYRVEGGSVLEG
                     GYLRDSGAGEVRVLFNEGSKVTLMRGARGRLRTVDEQGSRVVIDHGTASFEVARSNEF
                     HWFVEAGPFSVAVKGTQFTVSWDPVTEQFELNLRHGRVVVSGPVLEGDIELRAGRRLV
                     VNLARAETRITDEKPGTVNDPPKLDDPAARTAAPEAVHTPESSTPAPAMSAAVPPPAA
                     SSGKLGRGGWSEQLAKGRWNQILDEAERVGLQTTLETASSEDLFALADAARYRRRLDV
                     ARAALLAQRRRFPQSARSLDALFLLARVSESHDRARAIEGYDDYLRRSPSGTYAAEAL
                     GRKMVLTNELGGRSAAKPIAEEYLRRFPGGSYAGSARALSKP"
     gene            complement(2944..3576)
                     /locus_tag="VFQ35_16875"
     CDS             complement(2944..3576)
                     /locus_tag="VFQ35_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932382.1"
                     /translation="MSTRRSQASLALVGGGHPRPPSDAEVTDGLSAGEGWAVAAAWHR
                     FAPMVLTMAERALGSRSEAEDIAQEVFCAVYRKASTLREPDKLRSFVYSFAIRMLKSE
                     LRRRKVRSWLSFHTPEALVDMSSRSLDVESRDLLRRFYGVLERLSPRDRLVFTLRHME
                     SMTIEEVASTMNLSISTVKRSAKHAETRLSRWCRADAGLAHLFEQKRWDP"
BASE COUNT          641 a         1262 c         1188 g          549 t
ORIGIN      
        1 ccaggtcgtc gacgtccgcg acgcaacaag gaccgaggtt ccaagccgtg ccgagcgggc
       61 ccttcaatcc gtccggaaac gcccaaatca cgctctcggg gtcggtcagg gcggtgaaag
      121 ccgagttggc gagctcactc gaacctgagc ttcccatgcc gtgaaagtcg acgacgaggg
      181 ggacgcgacg cgagccatcg taagtcgacg gcacgtgcag gacgaagctg cgcgtctttc
      241 caccgatgcg cagtgtctcg accgtgtcac ccgggcgcag cgaggttgcg gggcacgccg
      301 ctccgccact tcccccactt gcgttgggcg ctccaccact cgagcgagtt cctccgcttg
      361 gagcggcacc gccgcccccg tgactcgcgc caccagacgg gggtgccccg cccgagccgg
      421 tggtgtcggc tccgctactc ccgccggagg attcggtgat ggcctgcaag tcagccggcg
      481 aacacgcaag aacagcgacg agcgcaagcg ctaccaacag ccagagcggc atgcgctcta
      541 gcggcgcgca gcgacgctgt cgatggccgg caccgtgtgc gatcggtgaa ctgtacttca
      601 tggccaagct ccgaacgcaa gcgcgacgac gaggttgggg cgccctgacg acgcgaccgc
      661 gtcatcgagg aagtgaatga ccacataggg ctgagcgagc tgcacctcgg ttacgagcga
      721 gagcgaatag tgtgcaccga gttcgagcga cgcgccgaca cctgcgtcga ccaaaaatga
      781 ccattggctc agcgagtgcc cctcgccggc gccagaggcg accccttcga ccgccgtgcg
      841 cagcgcgccc gcggacagcg cgaaaaaagg ccgtaaggag cgcttttcac ggaagccagc
      901 gcgggccccc acgagcacgt attgctgcgc gacctgcgcg ctccctgcgt tggtcgcgac
      961 gttgggcctc gttcccaatc cggccgcggt cgcttgaagc accactggcg cgtcgaggct
     1021 ccaattgacg cgagcgagcg gcaacacggc tgggcccagg cgatcgaagc tgatgagggt
     1081 cgccgcgcca atctcgaagc cgagccggcg gttgcgctcg tcttcgcgcg gcggcaccgg
     1141 attcttcggg cggagcgtgc ccggagtcgc cgctgcgtgc ttgggcggcg acacggatcg
     1201 gcgaggggtg aggtcgcctt cgaggaggcg cgagcgcaga acctcgacag cgcgaatcgc
     1261 gagctcgttg ggcgcgttct gatcggtcga cagctccacg agcgcgggct cgtaatcgcg
     1321 cttcgaatcc gcgatccaga catgaatcgc ctgcggattg acgtcgccca acacctcgag
     1381 caccgcttcc gcctccgagt cgcgcgcgag ctcgcggatt gcctccgact cggcgctcga
     1441 agagctggcg ggagacgcgg cggtgaagct cacttcgatg ccgatcgaca aaagctcgcc
     1501 atgcagacgc gcaagcactt cagcgacgct cggggagggg cgcggcggcc gcaacagcac
     1561 gaccttctgc gccaacgccg tcgtcgccca aagccaaacc acggaacaca gcgccgcgat
     1621 cgccagacga ttcacggcgc gctcacggct tggacagcgc gcgcgccgag cccgcgtagc
     1681 ttccccccgg gaaccgacgc aaatattctt ccgcgatggg cttggcggcg cttcgtccgc
     1741 cgagctcgtt cgtgaggacc atctttcgcc cgagtgcttc ggcggcgtag gttccgctgg
     1801 gcgagcggcg caggtagtcg tcgtatccct cgatggcgcg agcgcggtcg tgtgactccg
     1861 agacgcgagc gagcaaaaag agcgcatcga gtgagcgcgc cgattgcgga aagcgacggc
     1921 gttgagcgag aagcgcggcg cgcgcgacat cgagccggcg acgataccgc gcggcatcgg
     1981 cgagggcgaa caagtcttcg ctcgaggcgg tctcgagcgt cgtttggagc ccgacgcgct
     2041 cggcctcgtc caagatctgg ttccagcgcc cctttgcgag ctgctccgac catccgccgc
     2101 gaccaagctt cccactcgag gccgcaggcg gagggacggc cgcgctcatt gcaggcgccg
     2161 gcgtggacga ctcaggcgtg tgaaccgcct cgggagccgc agtccgggcg gcgggatcgt
     2221 cgagcttcgg gggatcattg acggtccccg gcttttcgtc cgtgatgcgt gtttcggcgc
     2281 gcgccaaatt gacgacgagc cggcgcccag cgcgaagctc gatgtctccc tcgagcaccg
     2341 gaccgctcac gacgaccctg ccgtggcgca agttgagctc gaattgctcg gtcaccggat
     2401 cccaagacac ggtaaattgc gtgcctttca cggcgaccga aaagggacct gcctcgacga
     2461 accagtgaaa ctcgttgctg cgcgcgactt cgaacgatgc cgtgccgtga tcgatgacga
     2521 cgcggctgcc ttgctcgtcg acggtgcgca accgcccccg cgcgccgcgc atcaacgtca
     2581 ccttgctgcc ctcgttgaac agcaccctca cctcgcccgc acccgaatcg cgcaggtagc
     2641 cgccttcgag cacgctgcca ccttcgactc gatacgagag tgccggcgga tggctcgtcc
     2701 aacgctggta cagagcgccg cttagccgta tgccccccac caaacaagca agggcggcga
     2761 cggccaccaa agagcggcgc acccagtgcg tccgccctgc ttcgcgcgcc gcgatgcgcg
     2821 ccaacagcac gttgagcccc ttgtcgagct cggccgcgct ggggcgctcg accgcgtcgc
     2881 gaccgagccg cgtcaacgcc gcaagcccgc gcgtctctcg cccctcgcgt tcgggtgccg
     2941 gggtcacggg tcccaccgtt tctgctcgaa gagatgcgca agccccgcgt cagcgcgaca
     3001 ccagcgcgac agccgcgttt ctgcgtgctt ggccgagcgc ttcaccgttg aaatcgagag
     3061 gttcatggtg ctggccacct cctcgatcgt catcgattcc atgtggcgca gcgtgaagac
     3121 gagccggtca cgcggcgaga gccgttcgag caccccgtag aacctgcgca gcaggtcgcg
     3181 tgactcgacg tcgagcgagc gtgagctcat gtcgacgagc gcctcgggcg tatgaaacga
     3241 gagccacgag cgcactttgc ggcggcgaag ctccgacttc aacatgcgga tcgcgaacga
     3301 gtagacgaag ctccgcagct tgtccggttc tcgcaacgtg ctcgccttgc ggtacacggc
     3361 acaaaacacc tcttgagcga tgtcttcggc ctcggagcgt gagcccaatg cccgctcggc
     3421 catcgtcaag accatcggcg caaagcgatg ccacgccgca gcaaccgccc aaccctcgcc
     3481 ggcagacagg ccgtccgtca cttccgcgtc gctcggcggc cgaggatgcc ctcccccaac
     3541 gagagccagg gaggcttgag aacgtcgcgt cgacatggat ccggtgctcg agcagtggca
     3601 accgaccctc caaactggct ctctttcttc tcccgcaggg
//
LOCUS       DASTKS010000817         5352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_576548, whole genome shotgun sequence.
ACCESSION   DASTKS010000817 DASTKS010000000
VERSION     DASTKS010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576548"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VFQ35_16880"
     CDS             complement(<1..1437)
                     /locus_tag="VFQ35_16880"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9932383.1"
                     /translation="MTHRWLYATAVVVAACASEPGAANGTGGTAGASGASTGGLASAS
                     GGVSGGSSEPDPDELLPARVRRLATAEYDASVQALLTTEQAIATGADFPPDLRQDGFT
                     VNGAQRVDAVSVERFADAADALATEAQQNGTLARLAPCERDADPASCARTFIETFGAK
                     VYRRPLLAEEVDALLALYAVGAEDATYEDGITHVTRGLLQSAGFLYLTELGDGAPSTS
                     GVVRMTPYELASELSYFVTSAPPDDELLAQAESGALSDPSERSAQARRLFRTDPRAQA
                     TAVRLVREWLGIDRIDASAKDSLIYPDFEAQKPRIVAESQDFVRAVTFQSTGTVAELI
                     GARWTVDTGPLGMYQAGARGPIAGSTLIDRVGILNQAAFLATYANAHESHPIFRGVAI
                     TRRVVCQHLDSPASFNLQVVPPPPDPSKTTRERYEVHAKDGLCAGCHDMIDPFGFAFE
                     HFDGMGAYRAKEHGRDVDSTVFVNLEGDY"
     gene            complement(1602..3248)
                     /locus_tag="VFQ35_16885"
     CDS             complement(1602..3248)
                     /locus_tag="VFQ35_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932384.1"
                     /translation="MAEELGTKSGALKVDAANELPSGTRRGFLQLGAVVVAGLVDGCS
                     GGTPEPGGEGGSPGKGGATGSGGGASGGAASGGTSNGQGGAASGGTSNGQGGASMGGR
                     QSSGGGTSTGTGGSTSSSSGGTTSSSGGTSSGVTGGTSPGGGATSGGSTGAGGGGSSG
                     GGWKESNTGGCTLGTVPSPTSNAKLPDPFKFISGQRLAKKEDWECLRAELSAMAQQCI
                     YGPKMPPPDKLTATFSGGTVTVNMTVGSTMKSFTFKISGAGSQSSPAPVLITCGGSSL
                     PKLSGVAQIDMSNNSFSSEKDNSGMVKDLYGSKAAKSGALISWAWGCSRIIDALELLP
                     EAGVDVKRIAVTGCSRNGKGALAMGAFDERVALTIPQEGGSGGPALWRVSEKEKAMGQ
                     NIQESGEIVGEAKWQGGDFPTYANGKNAMLLADQHTVIALCAPRAVLVIDNDIDWLGP
                     VACYGGGKAASKVYEGLGIKDRCGVSVAANHSHCQFPSAQQKFLDAFVNRFLKGMKVD
                     TSGVDDFHGTNSKLKTFDESMWIDWTVPTLSGSLSWDPFA"
     gene            complement(3971..4903)
                     /locus_tag="VFQ35_16890"
     CDS             complement(3971..4903)
                     /locus_tag="VFQ35_16890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9932385.1"
                     /translation="MVTREFPAVSVVIRSYDRLDACIELIENVLSQDHPSFEVVVVEQ
                     TPNPTAAQVARLTEFERDPRVRILRSAPLGAARARNVGWRSARNEVVLFMDDDDLPLR
                     ADWIRCHAQNFRDPLCVAVTGREVRSRDEDPSPYDTARARRLCLRYTFFKMPRGHTRH
                     TTRIEGVTAVQGGNASFRRAAIERAGGWDELTESTDENSFDFRFERVRRPGEYKVFDP
                     TPVMLRRLDQVGGLARRQASLSRVLSFELSYSHRLIRRYYPFRFYLCYFVYLWLAASR
                     AVSYVADHHRERSLLSLLRDLARYFAPAWRRAWA"
     gene            complement(4928..>5352)
                     /locus_tag="VFQ35_16895"
     CDS             complement(4928..>5352)
                     /locus_tag="VFQ35_16895"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HET9932386.1"
                     /translation="GRWKGRRPVVGYTVQRPEHWAFEGTGLERGDVFGADPTQPVVGY
                     ECDSAPFKIDADGTAVIIDPVSYATPATFEILGLAELNRAWSSRKGHSATMGAFSVDG
                     GGEVFNAATTDWVKLLDRDPAVTRITRNVLNRFARLDP"
BASE COUNT          909 a         1753 c         1715 g          975 t
ORIGIN      
        1 atagtcgccc tctaggttca cgaacacggt gctgtccacg tctcttccgt gctctttcgc
       61 gcgataggcg cccatgccgt cgaagtgctc gaaggcaaac ccgaacggat cgatcatgtc
      121 gtggcaaccg gcgcagagcc cgtctttcgc atgcacttcg tagcgctcgc gcgtcgtctt
      181 cgatggatcg ggtggcggag gaaccacctg caggttgaac gacgccggag agtccaagtg
      241 ctggcacacc acgcgccgag tgatcgcgac gcccctaaaa atcgggtggc tctcgtgagc
      301 gttcgcgtaa gtcgcgagga acgcggcttg attcaagatc cccacgcggt cgatgagcgt
      361 cgaccccgca atcggtcccc gcgcgcccgc ctgatacatg ccgagaggtc ccgtgtcgac
      421 cgtccagcga gcgccgatga gctccgcaac cgtgcccgtc gattggaagg tcacggcgcg
      481 cacgaagtct tggctttcgg ccacgatgcg tggtttctga gcctcgaaat ctggatagat
      541 gagcgaatct ttggcgctcg cgtcgatacg gtcgatgccg agccactcgc gtacgaggcg
      601 aaccgcggtt gcctgcgcgc gcggatcggt ccgaaacagg cgtcgcgctt gagcagagcg
      661 ctcgctcggg tccgacagcg caccgctctc cgcttgggcg agaagctcgt cgtcgggggg
      721 agccgacgtg acgaagtaag aaagctcgga cgcgagttcg tacggagtca tgcgcacgac
      781 gccgctcgtc gaaggcgcgc cgtctccgag ctcggtcagg tacaggaagc cagccgactg
      841 gagcagcccg cgcgtcacgt gagtgatgcc gtcttcgtac gtggcgtctt cggcgccgac
      901 ggcgtaaagc gcgagcagcg cgtcgacttc ttctgcgagt agcggccttc ggtagacttt
      961 ggcgccgaag gtttcgatga aagttcgcgc gcagctcgct ggatcggcgt cgcgctcgca
     1021 cggtgcgagg cgcgccaacg tgccgttctg ctgcgcctcg gtcgcgagtg cgtcggcggc
     1081 gtcggcgaat cgttcgacgc tcaccgcgtc gacacgctgt gcgccgttta cggtgaaccc
     1141 gtcttgccgg agatcgggcg ggaaatccgc gcccgttgcg atggcctgct cggtcgtgag
     1201 cagggcctgc acggaagcgt cgtactcggc ggtagccagg cgacgtacgc gagctggcaa
     1261 gagctcgtcc ggatccggtt cgctcgatcc gccgctcact cctccgctcg ctgacgcgag
     1321 tccgcccgtc gaagcgccgc tcgcgcccgc ggtgccgccg gtcccgtttg ccgcaccagg
     1381 ctccgaagcg caagccgcga cgacgaccgc ggtggcgtaa agccagcggt gcgtcatcgt
     1441 cattcgcttc tccggctcac gatttcccac actgacctct gggcggactg gggtcgagcg
     1501 gtctcggagc attcgagcct cgccatgagt cgcgaggctc taagggcgtg ttggtgggcg
     1561 ccgccgcaaa cgacgcgccc accagctccc atcgcgcacg ctcaggcgaa gggatcccaa
     1621 gaaaggctgc cgctcagcgt cggcaccgtc cagtcgatcc acatcgactc gtcgaaggtc
     1681 ttgagcttgc tgttcgtgcc gtggaaatcg tcgacgccgc tcgtatccac cttcatgccc
     1741 ttcaggaagc ggttcacgaa agcgtcgagg aacttctgct gggcggacgg gaactggcaa
     1801 tggctgtggt ttgccgcgac cgagacgcca cagcgatcct tgatgccgag gccctcgtac
     1861 actttggacg ccgctttgcc gccgccgtag caggcgaccg ggccgagcca gtcgatgtcg
     1921 ttgtcgatga cgagcacggc gcgcggagcg caaagggcga tcacggtgtg ctggtcggcc
     1981 agcagcatgg cgttcttgcc attcgcgtag gtcgggaagt cgccaccttg ccacttcgct
     2041 tcgccgacga tctcgccgga ctcctggatg ttctggccca tggccttctc cttttcggag
     2101 acgcgccaca gcgccggccc gccagagcct ccttcttgcg ggatcgtgag tgccacgcgt
     2161 tcatcgaacg cgcccatggc gagcgcgccc ttgccgttcc gcgagcatcc tgtgaccgca
     2221 atgcgcttca cgtcgacgcc cgcctcgggc agaagctcga gcgcgtcgat gatgcggcta
     2281 cagccccagg cccagctgat gagcgcgccg ctcttggcgg ccttggaccc gtacaggtct
     2341 ttgaccatgc cgctgttgtc tttctcgctc gagaagctgt tgttcgacat gtcgatctga
     2401 gcgactccgc tcagcttcgg caagctcgaa cctccgcacg taatgagcac cggcgccggg
     2461 ctcgactggc tgcccgcgcc gctgatcttg aaggtgaagg acttcatcgt gctgccgacc
     2521 gtcatgttga cggtgacggt gccaccgctg aaggttgcgg tcagcttgtc gggaggtggc
     2581 atcttcgggc catagatgca ctgctgagcc atcgcgctga gctccgcgcg caggcactcc
     2641 caatcttcct tcttggcgag acgctgcccg ctgatgaact tgaacgggtc gggcagcttc
     2701 gcattcgacg tcggagaggg caccgtgccg agcgtgcagc cacccgtgtt cgattccttc
     2761 cagccgccgc cgctactgcc accgccaccg gcgccagtgc tgccgccgct ggtcgcgcca
     2821 ccaccgggcg acgtgccgcc cgtgacgcct gaagaggtac cgcccgagct cgacgtcgta
     2881 ccgccagagc tcgagctcgt cgacccgccc gtgccggtag aagtgccgcc accggagctc
     2941 tgacggccgc ccatggacgc gccgccctgt ccgttgctcg tgccaccgct cgccgcgccg
     3001 ccttgcccat tgctcgtgcc gccgctcgcc gcaccaccgc tcgccccacc gccactgcct
     3061 gttgcgccgc ctttgcccgg ggagccaccc tctccacctg gttccggcgt tccccccgag
     3121 cagccgtcga cgagaccagc tacgacgacc gcgccaagct ggagaaagcc gcggcgtgtc
     3181 cccgagggga gctcattggc ggcatcgact ttgagagccc ctgacttcgt acctaattct
     3241 tcagccatga ttcctcgtcg gtgacaggcg acgcccacta ttcccggatg tgggtctagt
     3301 gatttgggaa aagcaataag gtccaataca ggacctcgat gcgtgcgttc gctaccccgc
     3361 actaacggcg atggccgata attcgaggca gcttggatcc ctcgttgcgt tggtgattcg
     3421 gacgttgcgc ctccgaatcg cgctcgaggg tctatttagg cgagcaatcg cgcacgacac
     3481 cgcgagttgg cggcatcagc gggacctctg ctacgttcgc attccacacg cattcgcgca
     3541 agtgccgtgt gtgtcactca ctcgcgcaag gacggtggtt cttacgacag gcgagcgcaa
     3601 cgaagcgact cgagccgtcg ccacgacaaa cttacgcgcg acgtgcagcg acgccacgct
     3661 gctcctcggt acccttcttc aaaggtgagt tctcctcggg cgtgcttctc gctcgcgtct
     3721 cttggctctc gcgacgggca ggaacttggg acaccccgta gtccggtcgt ggcttcgcga
     3781 cgtccgaagt cgtgagttca tgaacgcgcc gcgagagaca aaatatcctg gccttccgtg
     3841 gggcgggaaa gttgccagag caatagaagc gcggggcgga gcgcgtcagc ctggcgggct
     3901 ccgtcctcgc cgcaggggag aaacccgacg aagggcttac cgcttcgcgg ttactcgcgc
     3961 gtcgttcggc tcaagcccaa gcgcgacgcc acgccggcgc gaagtaacgc gccagatcgc
     4021 ggaggagcga gagcaacgat cgctcgcgat gatgatctgc cacgtacgag acggcgcggc
     4081 tcgcggcgag ccagagatac acgaagtaac agagatagaa gcggaagggg taatagcgtc
     4141 gtatcaagcg atgtgagtac gagagctcga agctcaatac gcgcgacaag ctagcctgac
     4201 gtcgcgcgag tccgcccact tggtcgagcc gtcgcagcat gaccggggtt gggtcgaata
     4261 ccttgtattc gccgggacgt cggacgcgct cgaatcgaaa gtcgaaggag ttttcgtccg
     4321 tgctctcggt gagctcgtcc cagccgcctg ctcgctcgat ggctgcccta cggaaagacg
     4381 cattcccgcc ctgaactgcg gtgacgccct cgatgcgcgt ggtgtgacga gtgtggccgc
     4441 gcggcatttt gaagaacgtg tagcggaggc aaagtcggcg ggctcgagcc gtgtcgtacg
     4501 ggctcggatc ttcgtcacgc gaccgcacct cgcgccccgt cactgcgacg cacagcgggt
     4561 ccctgaagtt ttgcgcgtgg caccggatcc aatccgcgcg aagcgggaga tcgtcgtcgt
     4621 ccatgaagag gaccacttca ttgcgtgcgc tccgccaacc cacgttgcga gcgcgggccg
     4681 cgccgagcgg cgcagagcgc aggatccgca cgcgagggtc tcgctcgaat tcggtcaggc
     4741 gggcgacttg agcggcagtg ggattggggg tctgctcgac gacgacgacc tcgaacgacg
     4801 ggtgatcctg cgagagcacg ttttcgatca actcgatgca agcgtcgagc cggtcgtagg
     4861 agcgaatcac gactgaaact gcggggaatt cacgtgtgac catgatgcaa tctctcggcc
     4921 gatcagctca aggatcgagc cgcgcaaagc ggttcaaaac gttccgtgta attcgagtta
     4981 cggccgggtc gcggtcgagc aacttgaccc aatcggtagt tgccgcgttg aacacctctc
     5041 caccgccatc tacggagaac gcgcccatcg ttgcagagtg cccttttcgg ctggaccacg
     5101 cccgattgag ctcggcgaga cctaagatct cgaacgtcgc tggcgtcgcg taactcacgg
     5161 gatcgatgat gacggcggtg ccgtctgcgt cgattttgaa aggtgcgctg tcgcactcgt
     5221 atccgaccac gggttgagtc gggtcggcac cgaacacgtc gcctcgctcg agaccggtcc
     5281 cttcgaatgc ccaatgctcg gggcgttgaa cggtgtaacc gacgacgggc cgacgcccct
     5341 tccatcgacc gc
//
LOCUS       DASTKS010000818         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1917320, whole genome shotgun sequence.
ACCESSION   DASTKS010000818 DASTKS010000000
VERSION     DASTKS010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917320"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..2060)
                     /locus_tag="VFQ35_16900"
     CDS             complement(90..2060)
                     /locus_tag="VFQ35_16900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9932387.1"
                     /translation="MASSSQPGGSRHVGRVIDPSVREDGEPEPAVELEPQQPWAESAG
                     ESASDAAGPTLPRGGRAREILLERAQSMRSTESASQDGSGLGDLDDIGKTDTAADPLE
                     AIGGSRAPSAPDSGVPEGRLSPNLLALFGTLLGIATVASLTALGMNLELKQSGSTTAP
                     SASAAVVAPAPAPEVVPKRQRTKQAGPWRIADARSDARMRVIEGKVGTDPFLKSLEAA
                     GVKTREIYRILTSMKGVRDFDRCKSSDRFAVLVERGSSKVKAFEYVTNPEDVYQSREG
                     DDGLLKGSKLDLKVERNQVTGALVFDGKSFDESAERGGFDKGLARVVAKALDGHMSLD
                     ELDRGARVRVIAQEVTVLGDFARYSGVEALEIVDADGKSFRLYYVEAPGERGYYDADG
                     HSPYEGGWRKPIKDAPMTSPYNLKRMHPILHKVVPHLGIDFGAPMGTPIGAASAGTIT
                     AASFVGPTGNFVRIQHANGIETGYAHLSRFAEGLKVGDKVKRLQVIGYVGSTGRSTGP
                     HLHFTARKNGEFFDPATLNLDAMRTLSKEARSMFSEVKAKYDAQLDKIRLPAPLAPEP
                     VAAAPSSDATPASAPAPSSASAAAAVASGSPPARDEAPEEDDEVPNAAAPAAAPAPAP
                     PGAKPSSGSSVYLSDQELLKLQGASDEGEVAE"
BASE COUNT          343 a          742 c          741 g          389 t
ORIGIN      
        1 cccccagggg agctcgttcg tcttcagcgt ctcactcgac gtgcgcaagc cgaagagcga
       61 acgtccaccg gcggtttgaa acggagtcgc tattcggcga cttcgccctc gtcgctcgcc
      121 ccttgaagct tcaagagctc ctgatccgac aggtacaccg agctacccga cgaaggcttt
      181 gcgccgggag gggcgggcgc cggggccgca gccggcgcgg ccgcgttcgg cacttcgtcg
      241 tcctcttcgg gtgcctcgtc gcgcgcgggc ggtgagccgc tcgcgaccgc ggccgccgca
      301 ctcgcgctac tcggagcagg cgcagacgcc ggggtggcgt cgctcgaggg tgcagccgcg
      361 accggctccg gcgcgagcgg cgctggcaaa cggatcttgt cgagctgcgc gtcgtacttc
      421 gctttgacct ccgaaaacat cgagcgcgcc tccttgctca gcgtgcgcat cgcgtcgagg
      481 ttcaaggttg cggggtcgaa gaactcgccg ttcttgcggg ccgtgaagtg gaggtgggga
      541 cccgtcgaac gcccggtaga gccgacgtag ccgatgactt gcagtcgctt caccttgtcg
      601 cccaccttca agccttccgc gaagcgcgaa agatgagcgt agccggtctc gattccgttc
      661 gcgtgctgaa tgcgcacgaa gttgccggtg ggaccgacga agctcgcggc cgtgatcgtc
      721 cccgccgacg ccgcgccgat aggcgttccc atcggcgcac cgaagtcgat gccgaggtgc
      781 ggtacgactt tgtgcaagat ggggtgcatg cgcttcaagt tgtagggcga cgtcatcggc
      841 gcgtccttga tcggcttgcg ccagccgcct tcgtaaggcg agtgcccatc cgcgtcgtag
      901 tagccgcgct cgccgggcgc ctcgacgtaa tagagccgaa aggacttgcc atcggcgtcg
      961 acgatttcga gcgcctcgac accggagtag cgcgcgaaat caccgagcac ggtgacctct
     1021 tgtgcgatga cgcgaacgcg cgcgcctcgg tcgagctcgt cgaggctcat gtggccgtcg
     1081 agcgccttgg cgacgacgcg cgcgaggccc ttgtcgaagc caccgcgctc ggcgctctcg
     1141 tcgaagctct tgccgtcgaa gacgagcgcg cccgtcacct ggttccgctc gactttgaga
     1201 tcgagcttgc tgcccttgag aaggccgtcg tcgccttcgc gcgactgata gacgtcttcg
     1261 gggttggtca cgtactcgaa agctttgacc ttcgagctgc cgcgctcgac gaggacggcg
     1321 aagcgatcgc tcgacttgca gcgatcgaaa tcgcgtaccc ccttcatcga cgtgaggatg
     1381 cggtagatct cgcgcgtctt gaccccagcg gcttcgagcg acttcaagaa cggatccgtc
     1441 ccgacctttc cttcgatgac tcgcatccgt gcgtcggagc gcgcgtcggc gatgcgccag
     1501 ggacccgctt gcttggtgcg ctgtcgcttt ggaacgactt cgggagcagg ggcgggagca
     1561 acgacggccg cgctcgcgga cggagccgtc gtcgagcccg actgcttgag ctcgaggttc
     1621 atcccgagcg cggtgagcga cgcgacggtc gcgatgccga gcagcgttcc gaacagcgcc
     1681 aagaggttcg gcgacaggcg gccctcgggc actcccgaat ccggcgccga aggtgcgcgg
     1741 cttccgccga tcgcctccag cgggtcggcc gccgtgtcgg ttttgccgat gtcgtcgagg
     1801 tcgccgagtc cgcttccgtc ctgactcgcg ctttccgtcg agcgcatact ctgggcgcgc
     1861 tcgagcaaga tctcgcgagc tcgcccgccg cgcggcagcg tcgggcccgc tgcgtctgac
     1921 gcagattcgc ccgcgctttc ggcccaaggc tgttgcggtt cgagctcgac agcgggctcg
     1981 ggctcgccgt cctcacggac cgacgggtcg atcacgcggc cgacatggcg cgagcccccg
     2041 ggctgcgatg aggacgccac tagaccgcct cggtagcagc ctccgttagg cgctgcaagt
     2101 tgcgcaccca caaaagggcg caacgaaacg cggcttcggt ggctttcagc ttcccaccag
     2161 ggcgaaagat cttggaaaat caacgcctcg ccatggatga ccacctgaag cagct
//
LOCUS       DASTKS010000819         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1246836, whole genome shotgun sequence.
ACCESSION   DASTKS010000819 DASTKS010000000
VERSION     DASTKS010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246836"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..951
                     /locus_tag="VFQ35_16905"
     CDS             64..951
                     /locus_tag="VFQ35_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9932388.1"
                     /translation="MADDSAAETVLSWCAKTTRSVVEQASAASERGVLEVGRALGVLV
                     EESRSQTSDLLEVLPRLEGDGAAHESSISIRDAIDRQTATVTTFAEQLISRAGRQNEI
                     AEHASKLTEGIAHLARDVSSIAVKARLLSLNARIECSRLGEQGKAMTVVANEIRQVSA
                     SVAKANADISELAGQLTELLPAVARLSAELQTSSQQFRESFEQSQSETQATYSDLMTL
                     VRSTVERSQTRAERMLSEAQDALSALNFQDPMAQQLRELAAKVEARCGNASAYTDGSP
                     SSSSKSDDSNMQAGEVALF"
     gene            1073..>2343
                     /locus_tag="VFQ35_16910"
     CDS             1073..>2343
                     /locus_tag="VFQ35_16910"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HET9932389.1"
                     /translation="MSASARVRLANEAPLAPRGQYVLYWMTSARRPAHNFALDHAIRL
                     ARELDKPLVVLEALTTSYRWASDRFHAFVVQGMADNRAAFAKSAAFYYPYVEPRAKAG
                     RGLVEALASRAAVVVADDFPCFFLPRLVASVAARIRVRCDAVDSNGIVPLAEVPREFS
                     RAFDFRRFLGKALVGHAQVAPVAEPLKHLRLPTLAELPVDIVTRWPIATDETLTGKAS
                     ALARLPIDHRVRPGLLPGGRRAGRRALERFCRERLDRYVDERNHPDCHATSELSPYLH
                     FGHISAHEVFAAVLAHDELSAKALEPAPSAQKSPFFQLRPGPAAFLDQLVTWRELGYN
                     FCSRRPDTYDRYEALPEWARRSLAQHQKDAREATYSLEELESARTGDPLWNAAQRELV
                     ESGEMHNYMRMLWGKLVIAWCKSPERAYELL"
BASE COUNT          411 a          753 c          770 g          409 t
ORIGIN      
        1 gaacggtgcg gaggcgatgc ccgagggtga agtcgcacta ttttgaggaa tcggggagct
       61 ctcttggctg acgatagtgc cgccgaaaca gtgctgagct ggtgcgccaa aacgacgcga
      121 tccgtcgtgg agcaagcttc ggcggcgagc gagcgcggcg tcctcgaggt ggggcgcgcg
      181 ctcggagtgt tggtcgagga gtcgcgctca cagaccagcg acctcctgga ggtgcttcct
      241 cggctcgagg gcgacggtgc cgcgcacgag tccagcatca gcattcgcga cgccatcgat
      301 cgccagaccg cgaccgtgac gacgttcgcg gagcagctga tatcgcgcgc gggcagacag
      361 aacgaaatcg ccgagcacgc ctccaagctg acggagggga tcgctcattt ggcgcgcgac
      421 gtttcgagca tcgcagtgaa agcgcggcta ctcagcttga atgcgcgcat cgagtgcagc
      481 cggctcgggg agcagggcaa agcgatgacg gtcgttgcga acgagattcg gcaggtcagc
      541 gcctccgtcg ccaaagcaaa cgccgatatt tccgagctcg ccgggcagct cacggagctc
      601 ttgcccgcgg tcgcccgcct ctcggccgag ctccaaacca gctcgcagca atttcgtgag
      661 tcgttcgagc aatcgcagtc cgaaactcaa gccacctact cggatctcat gactctcgtg
      721 cgctcgaccg tggagcgcag ccaaacgcgc gccgagcgca tgttgagtga agcgcaagac
      781 gcgctctcgg ccctgaactt ccaggacccc atggcgcagc aactgcgcga gctcgccgcg
      841 aaagtggagg ctcgctgcgg caacgcatcg gcctacacgg acggctcacc ctccagctct
      901 tcgaagtcag acgactcgaa catgcaggcg ggtgaggtcg cgctgttcta ggtctgtacg
      961 agtccgacga tcacgggccc gcaagtggga caagccgcat cgccggccga aatcgaagcg
     1021 tgtgagccac ggggattgtg gtgactcgat ccgttcacca tgattgtccg aaatgtcggc
     1081 ttcggctcgc gtgcgtttgg cgaacgaggc gccccttgcg ccgcgtggac agtacgttct
     1141 ctattggatg acgtcggcgc gccgaccggc gcacaatttc gcgctcgacc acgccattcg
     1201 gcttgcgcgg gagctcgaca agccgctcgt cgtgctcgag gcgttgacca ccagttaccg
     1261 ctgggcgagc gatcgctttc acgcattcgt cgtccagggg atggccgata atcgcgccgc
     1321 tttcgcgaag agcgcggcgt tttactatcc ctatgtcgag cccagggcca aggctggacg
     1381 cggactagtg gaggcgctcg cgtcgcgggc ggcggtcgtc gttgccgacg actttccctg
     1441 tttcttcttg ccgcgactcg tcgcgagcgt cgccgcgagg atcagagtgc gctgtgacgc
     1501 ggtcgattcc aacggcatcg taccattggc agaggtcccg cgggagttct cgcgcgcgtt
     1561 cgactttcgg cgttttctcg gtaaggcgtt ggtcggccac gcgcaggtgg cgcccgttgc
     1621 cgagccgctc aagcatttgc gcctgccgac gctggcggag ctgccggtcg acatcgtaac
     1681 gcgttggccg atcgcgaccg acgagacgtt gacgggcaaa gcctcggcgc ttgctcgctt
     1741 gcccatcgat caccgcgttc gcccgggact tctccctggg ggccggcgcg cgggacggcg
     1801 cgcgctcgag cgcttttgtc gagagcgcct cgaccgctac gtcgacgagc gcaaccatcc
     1861 ggactgccac gcgacgagcg agctctcacc gtacctccac ttcggacaca tcagcgcgca
     1921 cgaagtcttc gcagcggtgc tggcccatga cgagctctcg gcgaaggcgc tcgagcccgc
     1981 tcccagcgcg caaaagagcc cgttctttca gcttcggccc ggtcccgcgg cgttcctcga
     2041 tcagctcgtg acgtggcggg agctcggata caatttctgc tcgaggcgac cggacactta
     2101 cgaccgctac gaggcactgc cggagtgggc tcgccgctcg ctcgctcagc accagaagga
     2161 cgctcgagaa gcgacttatt cgctcgagga gctcgagtcg gcccgcaccg gagacccgct
     2221 ttggaatgcc gcgcaacgcg agctcgtcga gagtggagag atgcacaact acatgcgcat
     2281 gctctgggga aagctcgtca tcgcatggtg caaaagcccg gagcgcgcgt acgagctctt
     2341 gat
//
LOCUS       DASTKS010000820         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_799738, whole genome shotgun sequence.
ACCESSION   DASTKS010000820 DASTKS010000000
VERSION     DASTKS010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799738"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFQ35_16915"
     CDS             <1..553
                     /locus_tag="VFQ35_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932390.1"
                     /translation="ATHGAGGAPEWECEYWRRLTQGRWFIACLRGEPIDRRGSGSYYY
                     KNHLVLGREFRALNDALQQSFAGQLGTGFAVYAGFSQGAIMGAPMIVAHAARFRRLAL
                     IEGGYEYWSLSTARAFARNGGERVLFACGTRWCAQKAELPASWLRQAKVDVRIEYAEG
                     AGHTPVGEVMSKVQASLPWLLGP"
     gene            595..1752
                     /locus_tag="VFQ35_16920"
     CDS             595..1752
                     /locus_tag="VFQ35_16920"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9932391.1"
                     /translation="MDRTDVSARRALLVMLLSSLVLVLAVFRPLGAALFLAAVLAMVL
                     WPLHQWLTRRLRGRSSAAAGILVAAVVAIVLGPLVGLSAFVVNETAGATRFVSETVRS
                     EGTRGLLAKLPDPIEEQLTRLLSRVDADTGEVTKWVQQQVTQAGGSAALVVGAAVSAT
                     GSLLFQGVMMILALFFFMTNKEGILNWLDEASPLRSGQTRELFAEFVRVCKSVIVSTA
                     ATSLVQAIAALIGYLIARVPHPFFFFAVTFVIAFIPAAGAASVCLLAAAIQLVTGHPW
                     AALFLAIYAFLVVGLVDNVVKPLLMKDSIHMHGAVLFFALLGGLTAFGAMGMLIGPLA
                     VALFLAMLRIYQRDYSNEPKEREAMRREHVGSTESLATSSPRAEAGGSAGG"
     gene            2080..>2540
                     /locus_tag="VFQ35_16925"
     CDS             2080..>2540
                     /locus_tag="VFQ35_16925"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9932392.1"
                     /translation="MKTRDLSRREVLVRAAVGVASLNVAAALTALSRTARADDDGEGD
                     VGQLNVALGAEYEAIATYTSAAPLLAQDATMTKAARDALAALTAQFQAHHVEHAAALK
                     KLIQSSGGTPRADSGKPNLPASFPTAAKAMDVVKLAADREKHAAIAYARALS"
BASE COUNT          400 a          814 c          849 g          477 t
ORIGIN      
        1 ggcgacccac ggcgcaggtg gcgcgccgga atgggaatgt gagtattggc gtcggctgac
       61 ccaaggccgc tggttcatcg cgtgtctgcg tggggagccg atcgatcgac gcgggagcgg
      121 cagctactac tacaagaatc atttggtgct cggccgcgag tttcgagcat tgaacgacgc
      181 gctccagcaa agcttcgcgg gccagctcgg caccggattc gccgtttacg caggcttctc
      241 gcagggggcg atcatgggcg cgccgatgat cgtggcgcac gcggcccgct ttcgccgact
      301 ggcactcatc gagggcggct acgaatactg gagcctctcg accgcgcgag ccttcgctcg
      361 aaacggaggt gagcgggtgc tctttgcgtg cggcacgcgc tggtgcgctc aaaaggccga
      421 gctccccgcg agctggctgc gtcaggccaa agtcgacgtc cgcatcgaat atgccgaagg
      481 cgcgggtcat acgccggtcg gtgaggtgat gtcgaaggtg caagcctccc tcccctggtt
      541 gctcgggcca tgacgctcct tgcggcagcc gcagcgtcag cgcacgcttc ggcaatggat
      601 cgcacggacg tttcggctcg gcgcgcgctc ttggtcatgc tgctctcgag ccttgttcta
      661 gtgctggccg tatttcgccc gttgggggcg gcgctgttct tggccgccgt cctcgcgatg
      721 gtgctctggc cgttgcacca gtggctcacc cggcgcttgc gcggccgctc gagcgccgcg
      781 gccggcattt tggtggccgc cgtcgtcgcg atcgtgcttg gacctttggt cggcttgtct
      841 gcgttcgtcg tgaacgaaac cgcaggcgcg acgcgcttcg tgtccgagac ggtgcgcagc
      901 gaaggtacgc gtggtctcct cgccaagctt ccggatccga tcgaagagca gctgacgcgt
      961 ttgctctcac gcgtcgacgc cgacaccggc gaagtcacga agtgggtaca gcagcaagtc
     1021 acgcaagcgg gcggaagcgc ggccctggtc gtcggcgcgg ccgtatcggc cacgggctcg
     1081 ctcttgtttc agggtgtcat gatgatcttg gccttgttct tcttcatgac gaacaaggag
     1141 ggcatcctca actggctcga cgaggcgtcg ccgctacgca gcggccagac gcgcgagctc
     1201 ttcgcggagt tcgtgcgtgt ctgcaagtcg gtgatcgttt cgaccgcggc gacttcgttg
     1261 gtgcaagcga tcgcggcgct gatcggctat ctcatcgcgc gggttcccca cccgttcttc
     1321 tttttcgcgg ttacgttcgt catcgcgttc atccctgccg cgggcgccgc atccgtctgc
     1381 ctcctcgcgg cggcgatcca actcgtgacg gggcacccgt gggctgcgct gttcctcgcg
     1441 atttatgcct tcttggtcgt cggcttggtc gacaacgtgg tcaagccgct cttgatgaag
     1501 gacagcatcc acatgcacgg cgcggtactg ttcttcgctc tcttgggtgg actcaccgcg
     1561 ttcggcgcga tgggaatgct gattgggccg ctcgcggtcg cgctgttctt ggcgatgctt
     1621 cgcatttatc agcgcgatta ctcgaacgag ccgaaagagc gcgaggccat gcgccgcgag
     1681 cacgtcggct cgaccgagtc actcgccacg tcttcgccaa gagcagaggc aggcggaagc
     1741 gctgggggct gaggcgacga gcgcgcgcgg agcgagctct cggggctccg gctccgggtc
     1801 gaagttcgcc acgatacgga gttcgccacg gagacggact ccgtcagcgc gtccgggcac
     1861 gaccggccgt gcgaagccaa ggttttgcac gggagcgcgc tgacgattgt tgattatcgc
     1921 gtgtcgaggc tgtcgattgt tcgaatgtcg ccattttcag agacactcgc gcgcacgtat
     1981 cctccgaccg aaagcatgcg agtgagttcg gcgtcatacc gccgacgatt tccgctggat
     2041 gagcatgcga cttcgtctga cgcacatacc ggaggataga tgaagactcg agacttgagc
     2101 cgacgtgagg tgctggtgcg cgccgcggtc ggcgtcgcca gcctgaacgt cgcggcggcg
     2161 ctcacggcct tgagccgaac ggcgcgcgct gacgacgacg gagaaggcga cgtcggccag
     2221 cttaacgtcg cgctcggcgc agagtacgag gctatcgcga cgtacacgag cgcggcgccg
     2281 ctactcgcgc aagacgccac gatgaccaag gccgcgcgcg acgctctggc cgccctcacc
     2341 gcgcaattcc aagctcacca cgtcgagcac gccgcggcgc tgaagaaact cattcagtcg
     2401 agcggcggca cgccacgcgc cgacagcggt aaacccaact tgccggcctc gttccccacc
     2461 gccgcgaaag caatggacgt cgtcaagctc gccgccgacc gcgagaagca cgccgcgatc
     2521 gcgtacgctc gagcgctctc
//
LOCUS       DASTKS010000821         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1247404, whole genome shotgun sequence.
ACCESSION   DASTKS010000821 DASTKS010000000
VERSION     DASTKS010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247404"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFQ35_16930"
     CDS             <1..537
                     /locus_tag="VFQ35_16930"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9932393.1"
                     /translation="AELVRRAADGDAWAEEAIYRRYVTLVLGTSRRLLADNSDAEDVA
                     QETFATAFGAWSQLREPERLRQWLLQIAVRKVHRRFRRRKLLRALGFDDGPNDATLDV
                     LARTDCPQETRMELSLLAKALDKLSVNDRVAWMLRHVEGLSLEEVAQECGCSLSAAKR
                     RITSASEKIARFAAKGQP"
     gene            534..1661
                     /locus_tag="VFQ35_16935"
     CDS             534..1661
                     /locus_tag="VFQ35_16935"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET9932394.1"
                     /translation="MKLLHESMQEPIREVLDDSVSEEVVQRVWKGVRRRRSAGASSGW
                     RFERRYWAVFASAAMAMAFVFWFLRAPSKYQGPLLEANGQVLNSLGADSASSTLLNDG
                     SRILLGPGAALEVLDNDESAFVCVLRRGRGTFEVKPGGPRRWRVEAGPLRVEVIGTRF
                     TVDRSKSRVSVDVDHGTVLVRGDRVPDGVQKLTAGSRLVIDTAERASAERTSSAAPSA
                     GPGPAGAGSMVAASSGEVSTAKSSSAAASAQAPSAKIDLLQLADEQRRHGNVKGAIAT
                     LRTASSEGGDSARRAIAAFTLGRLLLDSAGRPAEAAQAFRSCLRLAPPAAIAEDAAAR
                     LVEALARSGNRDAAQRSAREYERRYPSGRRLADVRRWAAPG"
     gene            1677..>2083
                     /locus_tag="VFQ35_16940"
     CDS             1677..>2083
                     /locus_tag="VFQ35_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932395.1"
                     /translation="MQGSPTLGRGDIAKTFGIVLGIAAFAFCKAAGAVPNERATGERA
                     NRDAPGDVLRVAFSDCEAEPLGKQAFLKVLSLELGRRQLRESSSEAAALSVVYRCDGS
                     AHVRVASTSPEVDRELRVDDVAPRERARALALAV"
BASE COUNT          314 a          657 c          762 g          350 t
ORIGIN      
        1 gcggagctcg tgcgccgcgc cgcggacgga gacgcgtggg cagaagaggc catctatcgt
       61 cgttacgtca cgctggtgct cggcacttcg cggcgtttgc tggccgacaa cagcgacgca
      121 gaagacgtcg cgcaagagac cttcgcgacc gcgttcggcg cgtggtccca gctgcgtgag
      181 cccgagcggc tccggcaatg gctcttgcag attgccgtgc gcaaggtgca tcggcgcttc
      241 cggcggcgga agctgttgcg cgcgctcggc ttcgacgacg gtccgaacga cgcgaccctc
      301 gatgttttgg cgcggacgga ttgtccgcaa gagactcgga tggagctttc gttgctggcg
      361 aaggcgctcg acaagctctc cgtgaacgat cgtgtcgcct ggatgttgcg gcacgtcgaa
      421 ggtttatccc tcgaagaggt ggcgcaagag tgtgggtgct cgctctcggc ggcgaagcga
      481 cgcatcacct ccgcgtcgga gaagatcgcg cgctttgcag caaaggggca gccatgaagt
      541 tgctgcacga atcgatgcaa gagccaattc gcgaagtctt ggacgactcc gtcagcgaag
      601 aggtcgttca acgcgtttgg aagggcgttc gacggcgccg ctccgcgggc gcttcgagtg
      661 ggtggcgctt cgaacgacgc tactgggcag tgttcgcgag cgcggccatg gcgatggctt
      721 tcgtgttttg gttcctgcgc gcgccatcga agtaccaggg tcccttgctc gaggcgaacg
      781 gacaggtgtt gaactcgctc ggtgcggatt cggcgagctc cactttgctg aacgatggct
      841 cccgcatttt gctcgggccg ggcgcggcgc tcgaggtgct cgacaacgac gagagcgcct
      901 tcgtctgcgt gcttcgtcgt ggccgcggta cgttcgaggt gaagcccggc gggccccgcc
      961 gctggcgcgt cgaagccgga ccgttgcgcg tggaggtcat cggtactcgc ttcaccgtcg
     1021 atcggagcaa atcacgcgtg agcgtcgacg tggaccacgg tacggtgttg gtgcgcggcg
     1081 atcgcgtgcc ggacggcgtg caaaagctca ccgccggctc gcgcctcgtg atcgataccg
     1141 ctgagcgtgc gtcggcggag cgcacctcga gcgcagcgcc gagcgcgggt cctggccctg
     1201 cgggcgcggg gtcgatggtc gcagcgagca gcggcgaggt gtcgacggca aagtcgagct
     1261 cggccgccgc gagcgcccaa gccccgagcg cgaagatcga cctcctgcag ctcgctgacg
     1321 agcagcgtcg tcacggcaac gtcaagggtg ccatcgcgac tttgcggacg gcgtcgagcg
     1381 aaggtggcga ctccgcgcgt cgtgccatcg cggcgttcac gctgggcagg ttgttgctcg
     1441 acagcgcagg tcggcccgct gaagcggcgc aagccttccg ctcgtgcctg cgtctcgcgc
     1501 cgccggcagc cattgccgag gacgcggctg cgcgtttggt cgaggctttg gctcgctcgg
     1561 ggaaccgaga cgccgcgcaa cgaagcgcgc gcgaatacga gcgccgctac ccgagcggcc
     1621 gccgcctggc agatgttcgt cgctgggcgg ccccgggctg agctacgcta cgcaccgtgc
     1681 aaggctcgcc gacgctcggg cgtggtgaca tcgcgaagac gttcggcata gtcctcggca
     1741 tcgccgcgtt cgcgttctgt aaggcagccg gcgccgtgcc gaacgagcga gcgacgggcg
     1801 agcgcgccaa ccgggatgct ccaggcgacg tgctgcgcgt cgccttcagc gattgcgagg
     1861 cggagccgct cggcaagcaa gcgttcctga aagtgctgtc gcttgagcta ggccgtcgcc
     1921 agctgcgtga gagctcttcg gaggccgcgg cgctcagcgt cgtctatcgc tgcgacggca
     1981 gcgcccacgt gcgagtggcg tcgacctcac ccgaggtcga tcgggagctg cgggtggacg
     2041 acgtcgcacc gcgtgagcga gcacgcgcac tggcgctggc ggt
//
LOCUS       DASTKS010000822         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_132122, whole genome shotgun sequence.
ACCESSION   DASTKS010000822 DASTKS010000000
VERSION     DASTKS010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132122"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFQ35_16945"
     CDS             complement(<1..532)
                     /locus_tag="VFQ35_16945"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932396.1"
                     /translation="MMSTTSVTTLGIGVGDVIAEKYRIESVLGAGGMGVVFSARHTTL
                     NGQFALKFLSRDGLQSSQAIGRFYREAQAAARLRSEHAVRVFDVGTHTNGLPFIVMEY
                     LEGADLGRVLRASGQLPVTEAVDYTLQTCAAIADAHKAGIIHRDLKPSNLFCVTREDK
                     SVIVKVLDFGISKVTST"
     gene            1418..2212
                     /locus_tag="VFQ35_16950"
     CDS             1418..2212
                     /locus_tag="VFQ35_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932397.1"
                     /translation="MALLSSCSLVVDANRTQCSTNADCAAYANSVCVDSLCQPEPKWK
                     CLGKPIQQPAATGKFQVDFLVRHLVTQMPLPGVSARTCRKLDVECAAQPGEQLVTDES
                     GKVTVSVDAGFEGYVRFEGPSIAPGLYFFNPGVTSDLPQITISIGTIDVISLLALQAG
                     ARQSTDRGVALISVRNCVGGVAPGVTMASSVHDDVAVPFYSEQGLPSGSAVQTDDDSY
                     GGLLNASPGTHTFTATLAATGQRIGQATVLIQAGALTFGSVVPDGN"
     gene            2202..3923
                     /locus_tag="VFQ35_16955"
     CDS             2202..3923
                     /locus_tag="VFQ35_16955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932398.1"
                     /translation="METSSVSISRLQTRGSIGRHELVTALGEGGMAKVYLALQRGAFD
                     SAKLVVIKQIRPEFASDHDFLAMFVDESRIALRLNHPNVIHTYEVIAEHPDYYLTMEY
                     LEGQTLLQLVRRVGREAFPLLEHVWILTQVLAGLHHAHELRDLDGTPLGIVHRDVSPS
                     NIFVTSAGEVKLLDFGIAKAAGAVSLTQQGVIKGKLGYAAPEQCLAQPSDARSDIYAV
                     GVMLWEAIACRRRMAGESNMAALQARVTDGEPPIEQIVPEVAPELAAACRKALARDPR
                     NRYRSALEFQRALEAYLEQHQSTSTLAERVGALVREYFASELTHIRQVVETHVNESRR
                     SSARGLVPGTQRLTPLTDFAGAFDPRSDASNPGFRRGMSKRAWIGVGGGAALLALVGG
                     IWATQRSSAPHTAEAPSAGVPAGNATPSNAAVARVGGPTTAAAPVPSNINLLVAAGPA
                     RAHLRLDGKPIDNPFAGSFPADGHEHELVASAEGYQNEVRKLRFERDLEIRLSLSPII
                     PRGRAAAPSNNSAPEPKSAPAPAAAPPSPPPAPRSAPAAAVAQPGADLRKSPQRTSRT
                     IDDKDPY"
     gene            3926..4906
                     /locus_tag="VFQ35_16960"
     CDS             3926..4906
                     /locus_tag="VFQ35_16960"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9932399.1"
                     /translation="MKRIGVGLLFLQVCLSAPPALAESGQSSSSQVDQARGHFERGAA
                     LFKEGSFDAALAEFKRAYEIVPNYKVLYNIGQAQIERHDYVAALRAFEDYLRQGAADI
                     PSERRESVEREITSLKTRVASLVVRSNVRGAELFVDGVSVGTLPLDSDPLVSAGTRRL
                     TLKKTGYTQLERTVSVAGGDHPEIEMNLEPLPTTTGADAQPSGPVTPIAMPASRPIRP
                     ATWVSLAATCVFTGAAVTFGILAQKSNDDLDTAMRYPADPNRVDDVRSRLKLQAALTD
                     GFGAAAAASAILTVYFALSGSHSSETARAHRAPIARVVPTGQGVAVFGKF"
     gene            5015..>5999
                     /locus_tag="VFQ35_16965"
     CDS             5015..>5999
                     /locus_tag="VFQ35_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932400.1"
                     /translation="MKRAKGTVCLGLCAALWTAGCSDGQEAAARGDGQVATQSAALGR
                     GPKHPPHGADATPFPTRDPRALAFRERQVRKIYENLVFPAPIAILTGAQSVDDIFERE
                     VVKGRVTPVGQFLDFTAVVEYFYALAVTPGSQVQNIKFRSLFAGDDKVAVAVDIFFCR
                     APDQQCDPSVPVSPTSQTLTQVGFFQFNKQNRVISMDLNILNLGAASDPPNDPAAHAA
                     AIGNVCTALTRVHVGPTTGAVVSGGTCTGFFDSADDFSSGFVLSADPYENCVNFMQSI
                     PYGTWDRANSNSVTCRQLHTLLTAIDPEMHCPHASPSGGGKCIDHTYASYYD"
BASE COUNT         1045 a         1949 c         1917 g         1088 t
ORIGIN      
        1 gggtgctcgt cactttggag attccgaagt cgagcacctt gacgatgacg ctcttgtcct
       61 cccgggtgac gcagaacagg ttcgacggct tgagatcccg gtggatgatg ccggccttgt
      121 gagcgtcggc gatcgcggcg caggtttgca gcgtgtaatc gacggcttcg gttaccggaa
      181 gttgccccga ggcgcggagt acccgaccga gatccgctcc ttcgagatac tccatcacga
      241 tgaagggcag gccgttcgtg tgggtgccga catcgaagac gcggacggcg tgctcgctgc
      301 gcaatcgcgc ggcggcctga gcttctcgat aaaagcgccc gatggcctgc gaactctgga
      361 ggccatcgcg ggacaggaac ttgagggcga actggccgtt cagcgtcgtg tgacgcgccg
      421 agaagacgac gcccatgccg ccggcgccga gtaccgactc gattcggtac ttctcggcga
      481 tgacgtcgcc gacgccgatg ccgagggttg tcacactggt cgtactcatc atccgagcag
      541 tccgccgcgc ttcgcgagcc gacatcttaa tcgatgcgcc ttcgacgcgc cgagccgagg
      601 ccaaaaaacc ggggaagttc gtgcggaggt gcggcccaat tacaaccaaa gcggataggt
      661 ggtcctccgc caggaaccct cgtggcggcg aaagggggat ccttcgaaga agattccgca
      721 ggcaaagcga ttcgtggtat tcgccgtggc tcgccggtgg agccaagggt ggctaccaag
      781 caaccgcagc gcgcaagcgg gtctgctgcg gtgcgaaact cttgggagtg ggtccgcgcc
      841 gcgacctcat gacgcggagc tcgctgcgtc agtagcgggc tggaacgggt ttcagggccg
      901 gccgggtcag ttggtgcgcg gcagtggcac tcgcgagatg agaagcaaat tggcagtttc
      961 gctaggacga cgctctcgaa ttatgggagt ctcatgagtc tggggtgctt cgttaacgaa
     1021 ctcgaaatgc agctgaatct tcggcgcatt cgcgcattct tcgaaaagag tcccccagag
     1081 tcgcaaggct tcgacgcgct cgcgcgtagc cgagcttcgc atccgacatc ggcccgcttt
     1141 tccttgctgc atctacgtgc agcggttcgg cgcgtgaagc ctctcagttc atgaagtgac
     1201 tagtgtcgaa tgccgccagc attcttgaat cagcgagttc actgcgacgg gtgcgagcgt
     1261 cacctgcatg cgggccgcag aaagaagtcg tatgccgtct cgtcaggtta ttgacaagcc
     1321 atcgcgtgtc gagaaaagat tcgagtccac tcggaggagt tggtctgtcg tgaaaagcgg
     1381 tgtggtgagc cgactcattt ttcctttctg cttggcgatg gctttgctgt cttcgtgctc
     1441 gttggtcgtg gacgccaatc gcacgcagtg ctcgacgaac gccgattgcg cggcgtacgc
     1501 gaacagcgtt tgcgtcgact cgctgtgcca gcctgaaccg aagtggaagt gcctcggcaa
     1561 gccaattcag cagcctgccg cgactggcaa gttccaagtc gattttctgg tgaggcacct
     1621 cgtcacgcag atgccgttgc ccggcgtgag cgcgaggacg tgtcgcaagc tcgatgtcga
     1681 gtgcgccgcg caacccggcg aacagctcgt caccgacgag tccggaaagg tcaccgtctc
     1741 ggtcgacgcc ggcttcgaag ggtacgtgcg cttcgagggg ccgagcatcg ctccggggct
     1801 ctacttcttc aaccctgggg tcacctcgga cttgcctcag atcacgattt cgatcgggac
     1861 catcgacgtc atctcgctgc tcgcgctcca agccggcgct cggcaatcca ccgatcgcgg
     1921 ggtcgcgctc atctcggtgc gcaactgtgt cggcggggtt gcaccgggag tgacgatggc
     1981 ctcgagcgtt cacgacgacg tagccgttcc cttctactcc gagcagggct tgccttctgg
     2041 gtctgcggtt cagacggacg acgacagcta cggtggtctt ctgaacgcga gtccaggcac
     2101 tcataccttt acggcgacgc tcgcggctac gggtcagcgc attgggcagg ccacggtgct
     2161 gattcaggcc ggcgcgttga ccttcggaag cgtggttcca gatggaaact agctcagttt
     2221 cgatctcacg tttgcagacc cgcggctcga tcggccgcca cgaactcgtg accgcgttgg
     2281 gcgagggcgg catggccaag gtgtacctgg ccctgcagcg cggcgcgttc gactcggcca
     2341 agctggtcgt catcaagcag attcgcccag agtttgcttc ggatcacgac ttcctcgcga
     2401 tgttcgtgga cgagtcgcgt atcgccctcc ggctcaatca cccgaacgtc atccatacct
     2461 acgaagtcat cgccgagcac cccgactact acctgacgat ggagtacctc gaggggcaga
     2521 cgctactgca actcgtgcgg cgggtcgggc gggaggcatt ccccctcctc gagcacgtgt
     2581 ggattctcac gcaagtgctc gcgggcttgc accacgcgca cgagcttcgt gacctcgacg
     2641 gcacaccgct cggcatcgtg catcgcgacg tgagcccctc gaacatcttc gtcacgtcgg
     2701 cgggcgaggt aaagctcctc gacttcggca tcgccaaggc tgccggcgcc gtctcactga
     2761 ctcagcaagg cgtcatcaaa ggcaagctcg gttacgcggc gcccgagcaa tgtctcgccc
     2821 agccttccga cgcacgcagc gacatttacg ccgtcggcgt catgctgtgg gaggccatcg
     2881 cgtgccgaag gcggatggct ggtgagtcca acatggctgc gctgcaagcg cgcgtcaccg
     2941 acggcgagcc gccaatcgag cagatcgttc ccgaagtggc gcccgaactt gcggccgcgt
     3001 gccgcaaggc gctcgcgcgt gacccgcgga atcgctatcg ctccgccctc gagttccaaa
     3061 gagcgctcga ggcgtacctc gagcagcatc agtccacgtc gacgctcgcc gagcgcgtcg
     3121 gcgcgctcgt ccgcgagtac ttcgcttccg agctcacgca cattcgtcag gtcgtcgaga
     3181 cgcacgtgaa cgagagccgg cgttcgtcgg cgcgcgggct cgtccccggc acgcagcgcc
     3241 ttacccccct cactgatttc gcgggcgcgt tcgaccctcg cagtgacgcc tcgaacccgg
     3301 ggtttcgccg gggcatgtcg aagcgcgctt ggatcggcgt gggcggaggg gcggccctgc
     3361 tcgcattggt cggggggatt tgggcgacgc agcggagctc ggctccgcac acggcagaag
     3421 cgccgagcgc aggcgtcccc gccgggaacg cgactccgtc caatgcggcc gtcgctcggg
     3481 tcggcggccc cactaccgcc gctgctccgg tgccgagcaa catcaacctg ctggtcgctg
     3541 cggggccggc tcgcgctcac ttgcgtctcg atggcaagcc gatcgacaac ccgtttgcag
     3601 gtagcttccc cgccgacggc cacgagcacg agctcgtcgc gagcgcggag gggtaccaaa
     3661 acgaggttcg gaagctgcgc ttcgagcgcg atctcgagat tcggctgtcg ctctcgccca
     3721 tcattccgcg agggagagct gcggcaccgt ccaacaactc ggctccggaa cccaagtctg
     3781 cgcccgcacc tgcggctgcg ccgccttcac cgccgcccgc cccgcgttcc gcgccggccg
     3841 cagccgttgc gcaaccagga gccgatctcc gaaagtcccc ccaacgcacc agccgaacca
     3901 tcgacgataa ggatccgtac tgaccatgaa gcgaatcggc gtaggcttgc tgttcctcca
     3961 ggtttgcctc tcggcgccac ccgccttggc cgaatcgggc cagtcttcga gctcgcaggt
     4021 cgatcaggcg cgagggcact tcgagcgtgg cgcggcgctc ttcaaagagg ggagcttcga
     4081 cgcggcgctc gccgagttca agcgcgctta cgagatcgtc ccgaattaca aggtgctcta
     4141 caacatcggg caagcgcaga tcgagcggca cgactacgtc gccgcgctgc gcgctttcga
     4201 ggactatctt cggcaaggcg cggcagacat cccgagtgag cgccgcgagt cggtggagcg
     4261 cgaaatcacg agcttgaaga cccgcgtcgc gtcgctcgtg gtcaggagca acgtgcgtgg
     4321 cgccgagcta ttcgtggacg gcgtctcggt tggcacgttg cctctcgatt cggatccgct
     4381 cgtgagcgcc ggcacgcgcc gactcacgct gaagaagacg gggtataccc agctcgagcg
     4441 caccgtgagc gtcgctggcg gcgatcaccc agaaatcgag atgaacctcg agccgttgcc
     4501 cacgacgacc ggcgctgatg ctcagccctc ggggccggtg acaccgatcg cgatgcctgc
     4561 atcgcggccc attcgccctg caacttgggt gagcctcgcc gcgacgtgcg tcttcacggg
     4621 cgctgcggtc acgttcggta tcctcgctca gaaaagcaac gacgatctgg ataccgccat
     4681 gcgatacccc gctgatccga atcgggtcga cgacgtgcgt tcgcggttga agctgcaggc
     4741 cgcgctgacc gatggcttcg gcgccgcagc cgcggcgtcg gccatcctga ccgtttactt
     4801 cgcgctctct ggctcgcact cgagcgagac cgcgcgcgcc catcgcgcgc ccattgcgcg
     4861 cgtcgtgccg acggggcagg gcgtcgccgt gttcggcaag ttctgactcg agagcaaagc
     4921 cagctcgttc cgtcatttca cgcgcagaac gatcgtctgc acgtgaacgt gtgaccgcca
     4981 cgcccggcta gtccggcaac gaaagggagc agcaatgaaa cgagccaaag gaacggtgtg
     5041 tttggggctg tgtgccgcgc tttggaccgc gggatgcagc gatggccaag aggcagctgc
     5101 tcgaggcgat ggccaggtcg cgacccaaag cgcagccctg ggtcgcggac ccaaacaccc
     5161 gccccacgga gcggacgcga cgccctttcc gacgcgcgac ccgcgtgctc tggcgttccg
     5221 tgagcgacaa gtccgtaaga tctacgaaaa cctggttttc cctgcgccca tcgcaattct
     5281 gaccggcgcg cagagcgtgg acgacatctt cgagcgcgag gtcgtcaagg gacgcgtcac
     5341 acccgtgggg cagttcctcg atttcacggc cgtggtcgag tacttctacg ccctcgcggt
     5401 cacgcccggc tcccaggttc agaacatcaa gttccgctcg ttgttcgccg gcgacgacaa
     5461 ggtggcggtt gcggtcgaca tcttcttttg tcgcgcaccc gatcagcagt gcgacccgag
     5521 cgtgcccgtc tcgccgacgt cgcagacttt gacgcaggtc gggttcttcc agttcaacaa
     5581 gcagaaccgc gtcatctcga tggatttgaa catcttgaat ctgggcgcgg cttcggaccc
     5641 accgaacgac ccggctgccc acgccgcggc gattggaaac gtttgcaccg ctctcacccg
     5701 tgtccacgtc gggccgacta caggggcggt ggtgagcggc ggcacctgca cggggttctt
     5761 cgactccgcc gacgactttt cgtcggggtt cgtgctctcg gcggaccctt acgagaactg
     5821 cgtgaacttc atgcagagca tcccttacgg cacgtgggat agggcgaaca gcaacagcgt
     5881 tacctgccgc cagctccata cgttgctgac cgccatcgac cccgagatgc attgccctca
     5941 cgcgagcccg agcggtggag gcaagtgcat cgatcatacg tacgcctcgt actacgacg
//
LOCUS       DASTKS010000823         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_132229, whole genome shotgun sequence.
ACCESSION   DASTKS010000823 DASTKS010000000
VERSION     DASTKS010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132229"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFQ35_16970"
     CDS             complement(<1..408)
                     /locus_tag="VFQ35_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932401.1"
                     /translation="MRLPALSLALTLIATPHLAVAADAGGERTLSTEEVEAWLARPSG
                     APNQDTLAPSAEEAPPPPPRKHGFTIETGVGALTHLGPLSHVSPTAPWFHLQVGYEPF
                     RWLMVFAEGDLSFANTSYAAQPPPTRTYRLYGFG"
     gene            complement(483..1082)
                     /locus_tag="VFQ35_16975"
     CDS             complement(483..1082)
                     /locus_tag="VFQ35_16975"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9932402.1"
                     /translation="MSEPSVIGRGTVVRGNVRGDSSLEIQGRVDGDVTVDGDVVVGEE
                     AIVRGNITAAQLSVAGTVQGDLRGNESLLIERGARVVGDLSSPRIGVAPGALVRGNVR
                     TDGEAPLVSAQRRPGVGAQPLRSSPSAVRPVPAPAARPEPVRAAPPPPPPPPPEPEEE
                     AETESVPPSSAAPAAAKAPPPVVPALGKHAKAKKKRRDA"
     gene            complement(1091..1405)
                     /locus_tag="VFQ35_16980"
     CDS             complement(1091..1405)
                     /locus_tag="VFQ35_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9932403.1"
                     /translation="MAGTVVGEGLSIEGDLTSNEEVVVHGNVRGKLTTSDSVSIGGSG
                     VVQADVSGSSISIAGQVTGDVTASERVDLQAGGRLVGDVKAARFTIADGASFKGNVDM
                     EM"
     gene            complement(1441..1782)
                     /locus_tag="VFQ35_16985"
     CDS             complement(1441..1782)
                     /locus_tag="VFQ35_16985"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9932404.1"
                     /translation="MLGPGSKVTGRVSGEGSLRIEGSVRGEVQLSGDAEIAGGASVEG
                     NVQADAVDITGSLQGDVNARGPIAIRAGAVVRGELKGSEVSIEPGSRISVRLDTEIEL
                     DLGGGSAPKRR"
     gene            2094..3614
                     /locus_tag="VFQ35_16990"
     CDS             2094..3614
                     /locus_tag="VFQ35_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932405.1"
                     /translation="MRARASAPILTVALFTACGQIESDPQSSGGAPSFGGVVGRGGGV
                     GRGGKVGQGGEVGQGGSAGIASFGSGGRTVSGGAPGQGGAVGSGGRSGSGGSAQGGST
                     VVAGSAGAGGTGEAVLLESSWDTVMAIDVARSTSGVQLNCSQAAFAMHLTPNGAGVEV
                     IWGGNGDVMTGSLVGRFLGGPHYSFVRDFRIPTIGGDCGVASATVKAMRLDGSDLDAD
                     GFVDYIIGQGTGLASVLQGDVVESVEISFQFRAKTDLTPPTLVVPKLVHPLDGVVIRA
                     SEPLAPATVLTLNNGSDVIRMKDWDPAAGALVLFSASNILPFQSSWTVQGNATDLAAI
                     ALDFAKIPAVQVLDDPGVFAQDGFESTPKIATVGDVKLVSGVGNLPPIAGAQSLLVVP
                     NSSATFHLPRPSGASTVRLTLQRLTNVNYMSGGSPSIAAGVIGGTVRYPDSQSSTAAS
                     PAATGDATWAYASAKEDIKIPFTESGTDIVVRIAPFACSGLCPPAQALLIDDLRVE"
     gene            complement(4216..4428)
                     /locus_tag="VFQ35_16995"
     CDS             complement(4216..4428)
                     /locus_tag="VFQ35_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932406.1"
                     /translation="MNARLRVVCAWHWAVVEQGVFQRPAKSPRNGLRRSFDSLDCHIV
                     DEHRWVRRAQLEVQRSDAEAMSTLAP"
BASE COUNT          785 a         1524 c         1564 g          846 t
ORIGIN      
        1 gccgaaaccg tacaggcgat aggtgcgagt tggcggcggt tgcgcggcgt agctggtgtt
       61 cgcgaaactg aggtcgcctt ctgcgaacac catcaaccaa cggaagggct cgtagccaac
      121 ctggaggtgg aaccaaggcg cggtcgggct cacgtgcgaa agtggcccga ggtgcgtcag
      181 cgctccaacg cccgtctcga tcgtgaatcc gtgcttgcgc ggcggcggag gaggagcttc
      241 ctcggcactc ggcgcgagcg tgtcctgatt gggagcgccg ctcgggcggg cgagccatgc
      301 ctcgacttct tcggtcgaaa gggttcgctc gccaccagcg tccgctgcaa cggcgagatg
      361 aggcgtcgcg atgagcgtga gagcgagaga cagggccggg aggcgcaaga tagcctcaga
      421 gtaaccgagg ttctcgtgcc aaagccacga cttcgatgga tgggcggccg ttgggccgac
      481 tgtcaggcgt cgcggcgctt cttcttggct tttgcgtgtt tgccgagcgc cggaacgact
      541 ggtggcggcg ccttggccgc ggcgggagct gcggaagagg ggggaaccga ttcggtttcg
      601 gcttcctctt ctggctcggg gggaggcggc ggaggcgggg gcggagcagc tcggaccggc
      661 tccggtcggg cggcgggagc tgggacgggg cgcaccgcac tcggagacga gcgcaacggc
      721 tgtgcgccaa cgcccgggcg gcgctgagcg ctcacgagag gcgcctcgcc atcggtgcgg
      781 acgttgccac gcacgagcgc acccggagca acgccgatgc gcgggctcga tagatcgccg
      841 actacgcgcg cgccgcgctc gatgaggagc gactcgttgc cgcgcaagtc gccttgtacg
      901 gtgccggcga cgctcagctg cgccgcggtg atgttgccgc gcacaatcgc ttcctcgccg
      961 accaccacgt cgccgtccac ggtcacgtct ccatcgacgc gaccctgaat ctctaggctg
     1021 ctgtcgccgc gtacgttgcc gcgaacgacg gtaccgcgac cgatgacaga gggttccgac
     1081 atggatcgac tcacatttcc atgtcgacgt tgcccttgaa cgaggcaccg tccgcaatcg
     1141 tgaagcgcgc ggctttgacg tcacccacca agcgtccgcc cgcttgcagg tcgacgcgct
     1201 cgctggcggt tacgtcgccg gtgacttggc cggcgatgct gatgctcgag ccggacacgt
     1261 cggcttgaac gacgccgctt ccgccgatag agacgctgtc gctcgtcgtg agctttccgc
     1321 gcacgttccc gtggacgacg acctcttcgt tgctcgtgag atcgccttcg atgctgagcc
     1381 cttcaccaac caccgtgcct gccatatcgc ctctataatc ctcgcgttgc gcgtcgtggt
     1441 tcagcgacgc ttgggcgcgc tgccgccgcc gagatccagc tcgatttcag tgtcgagccg
     1501 aaccgaaatg cgtgagcccg gctcgatcga aacctcgctg cccttgagct cgccgcggac
     1561 tacggcgcct gctcggatcg caatcgggcc acgcgcgttg acgtcaccct gcagcgaccc
     1621 ggtgatatcc accgcgtcgg cctgaacgtt gccctcgacg ctggcgccgc ctgcaatctc
     1681 cgcgtcgccg ctgagttgaa cctcaccgcg caccgaaccc tcgatgcgca ggctgccttc
     1741 accgctcacc cggccggtca ctttgctgcc agggccgagc acactcagtt ctgtggaatg
     1801 tctgaccatt cgaacgcgcc gggacgctac actggaggga ggcatcgggg caacagctcc
     1861 aagcgcgacg cgctccattc gatccgcgag tcgtcagcgt gtgctcgccg ttcgagcgcg
     1921 atctttcttt cgtgcttcca agcatcgggg cgagccacgc acgtgggcag ctcgtggccc
     1981 ggcgcgccgt gctcgtcctt gtgccagctg tgccatctac gtagccgcgc tcccatgaaa
     2041 agcgctgcaa atctcggggt atgcgtggca cgtcggttgc ttcgtcgaca ctcatgagag
     2101 cgcgtgcctc cgccccgatc ctcaccgtcg ccctgttcac ggcttgcggc cagatcgaga
     2161 gcgaccccca gagcagtgga ggggctccat cgttcggagg agtggtcgga cgaggcgggg
     2221 gagtcggacg aggcgggaaa gtcggacagg gcggggaagt cggacaaggg gggagcgctg
     2281 gcatcgcgtc gttcggttcc ggagggcgta ccgtgagcgg tggcgctcca gggcaaggtg
     2341 gtgcagtggg gagcggcgga cgcagcggca gcggtgggag cgcgcagggc ggctcgaccg
     2401 tcgtcgctgg ctcggcgggc gcaggtggca cgggcgaagc cgtcctcctc gagagctcgt
     2461 gggacacggt gatggcaatc gacgtcgcgc gctcgacgag cggcgtgcaa ctgaactgca
     2521 gccaagctgc attcgcgatg cacctcaccc cgaacggggc cggcgtggaa gtcatctggg
     2581 gcggcaacgg ggacgtgatg acgggaagcc tcgtgggccg gtttcttggt ggcccccact
     2641 actccttcgt tcgtgacttc cggatcccca cgatcggcgg cgactgtggt gttgcgagcg
     2701 cgacggtcaa agccatgcgt ctcgatggca gtgatctcga tgcggacgga ttcgtcgact
     2761 acatcatcgg gcaaggaacc gggctcgctt cggtcttgca gggcgacgtc gtcgagagcg
     2821 tcgaaataag cttccagttc agagcaaaaa cggatctcac gccaccgacg ctcgtcgtcc
     2881 cgaagttggt gcacccgctc gatggggtcg tcattcgcgc ttccgagccg ctcgcgcccg
     2941 cgaccgtgct gacgttgaac aacggctctg acgtcatccg gatgaaggat tgggatcccg
     3001 ccgcgggggc gttggtattg ttctcggctt cgaacatttt gccctttcag agcagctgga
     3061 ccgtccaggg aaacgcgacg gatctcgcgg ccatcgcgct cgatttcgcg aagatccccg
     3121 ccgtgcaggt gctcgacgat ccgggggtgt ttgcgcaaga tggcttcgag tccacgccga
     3181 agatagcgac cgttggcgac gtcaaactcg tctcgggggt cgggaacttg ccacccatcg
     3241 ccggggctca gtcgcttctc gttgtcccga actcttcggc gacctttcac cttccgcgcc
     3301 cgtcgggagc gagcacggtt cgactcacgt tgcagaggtt gacgaacgtg aactacatga
     3361 gcggcggcag cccatccatc gccgctggtg tcatcggtgg cacggttcgc tacccggatt
     3421 cacaaagctc caccgcggcg tcccctgctg cgaccggcga cgcgacctgg gcatacgcat
     3481 ccgcgaaaga agacatcaag ataccgttca ccgagagcgg gaccgacatc gtcgttcgca
     3541 tcgcgccatt cgcttgcagt gggctctgcc ctcccgctca agcgctgctc atcgacgacc
     3601 tgcgggtcga gtgaggcaaa ccgcgtctcg gatggatgac tctggtcagc ccgggccgcg
     3661 ccgtgccgct acgcgccaac ttgtgccagc ggcaaagcca ggggctctct aggcagcgga
     3721 gcgactctgc ccgtcgcgct cgacgtcgag ctccacggcg ccttggatag gacgcctccg
     3781 tcgctgcggc tcgattcgga gcgtcacaac ccgctcgatg aggttcgcat cgcggcgacg
     3841 gagccgctgt tacctacgag cgaggccacc ttggtcaatg cgacggagca gtacgcatca
     3901 ttggttcgcc gaacggcgcg ttcatccaat tttcgacgaa cgtaattctg gcgttcggct
     3961 cgagcttcga cgtgcgcgtt ggcgcgtgat ctcggaggca tcgacctcca agctccagcg
     4021 agcccggcct tcgtggtgct cgacgatccg ggcgtcttcg acgaaggcag ctgcgaatcc
     4081 ggagaacgtg ctgttatcga gcggtgcgat gatcgtttca ggcgttgggg caccgcccgc
     4141 catcgctccc ttgaactacg aggtgcgtac gacgtctgaa accggagaca cgtcctggcc
     4201 ttacgccggc gacgttcaag gcgcgagcgt cgacatcgcc tccgcatcac tgcgctgtac
     4261 ttcgagctgt gcgcgccgga cccagcgatg ctcatcgacg atgtggcaat cgagtgaatc
     4321 aaacgagcga cgcagtccgt tgcgcggcga cttagcaggg cgttgaaaaa cgccctgctc
     4381 cacgacagcc caatgccagg cacacacgac gcgcagccgc gcattcatgg gctttcgtga
     4441 cgcgcggccg cgcgttggtg ccggtccgca ttctaacagg gcgctttttc aacgccctgt
     4501 cagggcccgc gaatgggtaa gtcatactgc gcgagccgag cctctcgctg cgacggacgg
     4561 gctcgtcgct gcgctcctcc gcccaattta gtctcaggat ttatcggatc tccgaaccac
     4621 gcatcggtcc gcccttttcg gggtttctgg ggccccgaaa accacacaaa ccaaagcata
     4681 ggaacgaact ctttcgaagc acgggtgaga accaactct
//
LOCUS       DASTKS010000824         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1918979, whole genome shotgun sequence.
ACCESSION   DASTKS010000824 DASTKS010000000
VERSION     DASTKS010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918979"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VFQ35_17000"
     CDS             <1..1767
                     /locus_tag="VFQ35_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932407.1"
                     /translation="QSNLSSLLNDFRDMVLAVGQAGCGLESSLEGWYRFLVDPQPLLA
                     TPQVTGGGATKPTYSDDIGSNPVLKQRAAFLRPDSAVVVVMLSDENDCSIIDEGAGWL
                     VGTPTLNGALFNMPRSTSACATNPNDPCCGSCIGTTLAAGCPAASQDAACQIGNYTAP
                     EQDALNLRCYRQKERFGLDLLYPTERYVKGLSEYQIQNRDGKMVENPLFANPSGPPRD
                     RSLVHLVGIVGVPWQDLAIDANASALEYKSYRDIDWELVLGSPGDATTPPTPPRDRLM
                     YESIADRTTLFGPAPHPVIGSAGALAPASSVGRPNAINGHESNIKNNDDLQYACIFQL
                     PSPRVECAGPGCDCDVVGQDYNRPVCDQSTQTFAKAYPGVRELQVLKDFGAASSGNVV
                     AASICPKTLSVSKTDPSYGYLPAVSALLAQLKPNFSPRCFPRQLATDATGRVPCSIIE
                     SRAPSAGQACGECDASARRSAASPSERNAVLEQLRATGHCGAAGQVACESLCLCALDQ
                     YEGSELLACQSAPTPPSAIASGFCYVDPAQASEPTLSLAESQVVANCPSSQKRMVRFL
                     GADTPAKDAVVSIACTGQTAQP"
     gene            1916..>2355
                     /locus_tag="VFQ35_17005"
     CDS             1916..>2355
                     /locus_tag="VFQ35_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932408.1"
                     /translation="MRAIFAFACGFGVIVGCGRAENDGAKGSGGNSAALGGAASGGAT
                     SGAGQSGEATFGGHIGSGGSSVANAGGGIVASGGNGGATAGAAENPCGAGDFFLKIDG
                     DLPGGFGANQRLSLGCPDARPGVFAVPVSFTRPPPEGGGIEVGIV"
BASE COUNT          415 a          766 c          763 g          411 t
ORIGIN      
        1 caaagcaacc tgtcatcctt gctcaacgac tttcgcgaca tggttctcgc cgtcggtcag
       61 gctggctgcg gcctcgagtc gagcctcgaa ggctggtatc gttttctcgt cgatccgcag
      121 ccgctcctcg ccaccccgca ggtgactggg ggcggtgcga ccaagccgac gtacagcgac
      181 gacatcggca gtaacccggt gttgaagcag cgcgccgcgt tcctgcgccc cgactcggcg
      241 gtcgtcgtcg tcatgctctc ggacgagaac gactgctcga tcatcgacga aggcgcaggc
      301 tggctcgttg ggacacctac gctgaacggt gcccttttca acatgccgcg ctcgactagc
      361 gcatgcgcta cgaacccgaa cgacccgtgt tgcgggtcct gtatcggcac cacacttgcc
      421 gcggggtgcc ccgcagcatc ccaagatgcg gcgtgccaga ttggcaatta cacggcgccc
      481 gagcaagacg cattgaactt gcgttgctac cggcagaaag agcgcttcgg gctcgacctg
      541 ctttatccaa cggagcgtta cgtgaagggc ctctcggagt accagattca aaaccgcgac
      601 gggaaaatgg tcgagaaccc actgttcgcg aacccgagcg gcccgccgcg cgatcgttcc
      661 ctcgttcacc tggtcggcat cgtgggtgtc ccttggcaag atctcgcgat cgatgcgaac
      721 gcgtccgcgc tcgagtacaa gagctatcgc gacatcgatt gggagctcgt cttgggctcg
      781 cccggtgacg ccacgacccc accgaccccg ccgcgggacc gcttgatgta cgaatcgatc
      841 gccgaccgga ccacgctctt cggtcccgct ccgcatccgg tgatcggcag tgcgggcgcg
      901 ctcgctccgg catcctccgt gggccgcccg aacgccatca atgggcacga gagcaacatc
      961 aagaacaacg atgatctgca atatgcgtgc atcttccagc taccgagtcc acgagtagag
     1021 tgcgcaggac cgggctgcga ctgtgatgtg gttgggcaag actacaatcg ccccgtttgc
     1081 gaccagagta cgcaaacctt tgcgaaggcc tacccgggcg ttcgggagct ccaggtcttg
     1141 aaggatttcg gggcggcgtc gtcgggcaat gtggtcgccg cctcgatttg cccaaagacg
     1201 ctcagcgtga gcaagacgga tccaagctac ggatacctcc ccgccgtttc ggcgctcctc
     1261 gcgcaattga agccgaactt ttctccgcgc tgtttccccc gccaactcgc gaccgacgcc
     1321 accggtcgag tgccctgctc aatcatcgag agtcgcgcgc cgagcgccgg gcaagcgtgc
     1381 ggcgaatgtg acgcctccgc gcggaggagc gcagcgtcac cgagcgagcg caacgccgtc
     1441 ctcgagcaac ttcgcgctac gggtcattgc ggcgccgcag gacaggttgc gtgcgagagc
     1501 ctctgtcttt gcgcgctcga ccaatacgaa gggagcgagc tgctggcttg ccaatccgcg
     1561 ccgacgccgc cgtctgcgat cgcttccggc ttttgctacg tcgaccccgc gcaagcgagc
     1621 gagcccaccc tgagcctcgc cgagagccag gtcgttgcga actgcccctc gagccagaag
     1681 cggatggtcc ggttcctcgg cgccgacacg ccggcgaagg acgcggtcgt gagcatcgcg
     1741 tgcaccgggc aaaccgctca gccgtaagcc aacgcgccgc tcgtcgaagc ggtcgacgaa
     1801 acatcgacag actcgcgcgc aagttcttcg ccagtgcgga tttgccgact acgaagtgcg
     1861 gtgacgctag gtcagctgct tgacctcggg cgtcgcgaac cgcacaatcg gtgtcgtgag
     1921 ggccatcttc gcgttcgcat gtggtttcgg cgtcatcgtc gggtgtgggc gcgcggaaaa
     1981 cgacggcgca aaagggtcgg gcggtaacag cgcggcgctc ggaggcgcag cgagtggcgg
     2041 tgcaacgtcg ggcgccgggc aatcgggaga ggctactttc ggcggccata ttggcagtgg
     2101 tggcagcagc gttgccaacg caggtggcgg tatcgtcgcg agcggtggca acggcggggc
     2161 gacggctgga gccgcggaaa atccctgcgg cgccggcgat ttcttcctga agatcgatgg
     2221 tgatctaccc ggcgggtttg gcgccaatca gcggttatca ctcggctgtc ccgatgcacg
     2281 cccaggggtg ttcgcggttc cagtgagctt cacgcggccg ccgcccgagg gcggtggcat
     2341 cgaggtcgga atcgt
//
LOCUS       DASTKS010000825         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1471964, whole genome shotgun sequence.
ACCESSION   DASTKS010000825 DASTKS010000000
VERSION     DASTKS010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471964"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /gene="pyrG"
                     /locus_tag="VFQ35_17010"
     CDS             <1..262
                     /gene="pyrG"
                     /locus_tag="VFQ35_17010"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238246.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HET9932409.1"
                     /translation="YREPLTRAGLSLSGTSPDDRLVEIVELNEHPYFIGCQFHPEFKS
                     RPLAAHPLFSQFVKAACRRRDDIARSVRGEGAPGHSSESAVN"
     gene            539..1783
                     /locus_tag="VFQ35_17015"
     CDS             539..1783
                     /locus_tag="VFQ35_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932410.1"
                     /translation="MAVVLAVGGCAPSGPVRAALYGDLPSLREEIRRAERANKLDRAE
                     VKRLALAVAEREAASANGSSGVRHVRSLSVCGSALLPALRRRSEHTDEVAAQAMRVRI
                     ALRDVSTASLVKDYTNAREGAWRAVAARAAVAAEDALQRRAWFTDPDQEVREAALEAA
                     AIAPVLEDREALLEVFRLDPDPICRSRAAHAAGALGGEESVLGLADRFARADSDGQLN
                     IVEAWAMPASYSSGGARELRRLASAGNGLPSIAAVEALLRQNDHDGALAGLLAMAIEH
                     GSEEEQRTALQQAPVADERVRGAVERASRDANPNISVLAWSRLLEVPAHSAAATNALR
                     IIAKKPIPAAAEARFTLASVGDASVVPELVAEAQSGAPWSRGRAAVALFRLGQPARAA
                     ATLADPDPGVRIATACGIVAAR"
     gene            2488..>2940
                     /locus_tag="VFQ35_17020"
     CDS             2488..>2940
                     /locus_tag="VFQ35_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932411.1"
                     /translation="MRHALKFLILSGVVVCASLAGCSTLSGDDENDGTSGAPSSGGGI
                     SIGNSGGSSGKGGASGMSSGGSGNMQPDMCPSFEGIDPNQCGSSSVEATVKTVNMMLV
                     IDKSGSMASGLDGTPEGMPSKWSGMQTALAQALAKVQDDINFGMVLFPA"
BASE COUNT          493 a          912 c         1006 g          529 t
ORIGIN      
        1 ctaccgcgag ccgctgacgc gcgctggcct gtcgctcagc ggaaccagcc cggacgatcg
       61 gctcgtcgaa atcgtcgagt tgaatgagca cccgtacttc atcggctgcc aattccaccc
      121 ggaattcaag agccgtccgc tcgccgccca ccccctgttc tcgcagttcg tgaaggccgc
      181 ctgccgccgt cgcgacgaca ttgcgcggtc ggtccgaggt gagggcgctc cgggccattc
      241 gagcgaatct gccgtcaact gaggcgttct cgcccaaccg gctcgtagct cggtcccacc
      301 attcgaggtg ccctgctcaa cggcggcgtg agcgagggca tgggtacggc attgctcctc
      361 ggcgagtctg gacttgccgc ggaggatgct cgaccgcggg ccgaggccgc gcggttctct
      421 ccgacgtttc aggatcgcgc gacggcctac taagatagcc gggatggtcg aagcgtcgcc
      481 tcagcaccct tccggccgaa cctttccccg gttccgagcc ctcgcatcga agttgacggt
      541 ggccgttgtg ctcgcggtcg gggggtgcgc cccgtcgggc cccgttcgtg cggcgctgta
      601 cggcgaccta ccgtcgttgc gtgaagagat ccgacgagcc gagcgcgcca acaagctcga
      661 tcgcgccgag gtgaagcgct tggcgctcgc agtggcagag cgcgaggcgg cttcggcgaa
      721 cggtagtagc ggagttcggc acgtgcgctc tctcagcgtt tgcggcagcg cgctgttgcc
      781 cgcgctacgg cgccgctcgg agcacaccga cgaggtcgct gcgcaagcga tgcgcgtgcg
      841 tatcgcgttg cgcgacgtgt ctacggcatc cctcgtcaaa gactacacga acgcaagaga
      901 gggcgcctgg cgcgctgttg ccgcgcgcgc ggcggtcgcg gccgaggacg ctctgcagcg
      961 ccgcgcgtgg tttacggatc cggatcagga agtccgcgaa gcagcgctcg aagcggccgc
     1021 gatagctcca gttctcgagg accgcgaagc gctgctcgag gtctttcgtc tggatcccga
     1081 cccgatttgc cgcagccgtg cagcacatgc cgcgggcgca ctgggcggag aagagagcgt
     1141 gctcggcctc gcggatcgtt tcgcgcgtgc cgactccgat ggacagctca acatcgtcga
     1201 ggcgtgggcg atgccggcct cgtattcgag cggcggcgcg cgcgagcttc ggcgcttggc
     1261 gagcgctggt aacggacttc cttcgatcgc cgcggtcgag gcgctattgc gccagaacga
     1321 tcatgacggc gctctcgcgg ggctcttggc gatggcgatc gaacacggga gtgaggagga
     1381 gcagcgcacg gcgttgcagc aggctcccgt cgccgacgaa cgcgttcgag gagctgtcga
     1441 gcgcgcgagc cgcgacgcga atccgaatat ctcggtgctc gcctggtcgc gcctcttgga
     1501 ggtccctgcg cattcggcgg ccgccacgaa cgcgctgcga atcatcgcaa agaagccgat
     1561 tccagccgcg gcagaggctc ggtttaccct ggcgtccgtt ggcgacgcga gtgtggttcc
     1621 ggaactggtg gcagaagccc agagcggggc gccgtggtcg cggggccgcg ctgcagtggc
     1681 actatttcgc ttgggccaac cagctcgcgc ggccgctacg ctggccgatc ctgatccggg
     1741 cgttcgtatc gcgacggctt gcggcatcgt tgcagcgcgc tgaggagatt tgatctcgtc
     1801 ttccgatgtg cgccaatcag cgtgcgacgg agacgcgcac gtacgcgcga gttccgcgcg
     1861 ggtcttcata aattctgcgg tgtcctccca tgtgagaggg cgttcgtttc ttgcggcctt
     1921 tggagccgaa aggcgcctat ttgccgagtg ggtcttcaag aatctggtcg gtgtccaccc
     1981 acacaccttg ggttcgcgac actcactgga aaacacattc gaaagcgggc ccgtcgaaat
     2041 tatcggattc gattgaacac gccgactacg ctcagaaatc aggcgaattt cgcgcgagcg
     2101 ccgcgcgagt cagcacgcta tataagaggt cagcggtctg cgcgcgcacg cagcaccgtc
     2161 gcgaccaaga gcgctggtcg tcacgatcgc ccgtcgcctt ttcatcttgg gttttccacc
     2221 atgccgagct cacttgaggc tgcatgccgc gggcgtttcc gtccgaacgg gcgcaacgct
     2281 cgcgagcgca ctttgggcgc gggtggcgaa gtgatcgcga tggttcagcg cagcgctgca
     2341 agctttcgac ttccgagcgc agcgccggtg aacgttcgac tacggagact gatccgtgat
     2401 tgcctgaaaa cgcactacgg agctaggctc tcgaaacggt gtggcagcat ggccgcacgc
     2461 cttatgggtg actagagagg acgaataatg cgtcacgctt tgaagtttct gatattgtcc
     2521 ggtgtcgtcg tatgtgcgtc gctggcgggc tgtagcaccc tgagtggcga cgacgaaaac
     2581 gatggcacga gcggcgcgcc gagctcgggg ggtggcatca gcatcggtaa ttcgggcggc
     2641 agctcaggca aaggcggtgc gagcgggatg agctcgggtg gtagcgggaa catgcagccg
     2701 gacatgtgcc cctcgttcga aggcatcgat ccgaaccagt gcgggtcgag cagcgtcgag
     2761 gccacggtga agaccgtgaa catgatgctg gtcatcgaca aatccggcag catggcgagc
     2821 ggcctcgatg gcaccccaga gggcatgccg agcaagtggt cgggaatgca aacggccttg
     2881 gctcaggccc tggccaaggt tcaggacgac atcaacttcg gtatggtgtt gttcccggcc
//
LOCUS       DASTKS010000826         6506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1472139, whole genome shotgun sequence.
ACCESSION   DASTKS010000826 DASTKS010000000
VERSION     DASTKS010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472139"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..2632
                     /locus_tag="VFQ35_17025"
     CDS             266..2632
                     /locus_tag="VFQ35_17025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9932412.1"
                     /translation="MVAPDFRVVFESLPGCYLVLSPELQILAVSDPYLRATLTTREQL
                     LGRGLFEAFPDNPDDPEATGVANLKASLQTVLSEHRAHEMTIQKYDIRRPESEGGGFE
                     ERYWKPVNSPVFDASGRIQCIVHQVEDVTPMMRLEKHGREQDRRIQELSIRGETQLRQ
                     LLDAAPDAMLVVGSDARIRFINAQAEQLFGYASSELLGRDLDLLVPSKFHVAHVEHLR
                     RFFREPRARSMGSGLELYARRKDGKEVPIEVSLSPLLLEEGAFVTAAIRDVTERKRIE
                     AAANLLASRLASAVESIQDAFALFDETDHLVLCNSAYRRLVNEAISGPLVGLTYEHIL
                     SAWLPQLDLGDEKSREAFFAERRAQRSEPHVDFKLRTVDGRSLRVIDQRTPEGGMVKT
                     IWDLTEDARIAAELKEARVAAEAASAAKSEFLSSMSHELRTPLNAILGFAQLLCRDKK
                     QPPSERQLERIRHILDGGEHLLKLIDDILDLSRIEAGRVSVSIEPVDVNLVLQELRTT
                     LGPSAAASEISLVVPTAASPPILADRTRFTQILMNFGSNAVKYNRPGGRVTFEVSEPA
                     PDVVRITVADTGLGIPSNKQGALFQPFQRAGQENGPIEGTGIGLAISKRLAEAMGGTV
                     GFHSEHGVGSRFWIEMPVHATHAPSSSPPKPRRGGEYGLERKEQSLVLYVEDNPANVR
                     FMEELLSAYENLELIVARTAEEGLEIARARRPRVIILDINLPGMSGLDALRVLRDRPE
                     TARTPVIALSAAASELDQKRGKLAGFASYLTKPLRVDEFESALSALLN"
     gene            2793..3182
                     /locus_tag="VFQ35_17030"
     CDS             2793..3182
                     /locus_tag="VFQ35_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9932413.1"
                     /translation="MSGPLQRILVAVDLSSCSRAVLEFACTLARPMGSTLEVLFVRPP
                     AQVDAAEELHRFVHPIVKDAGLTVIERVEAGDARERIVAIAAEEKFDLVAVGTHGRTG
                     RPRSLAGSVAESVVRTATCPVMTVRER"
     gene            3184..5733
                     /gene="mgtA"
                     /locus_tag="VFQ35_17035"
     CDS             3184..5733
                     /gene="mgtA"
                     /locus_tag="VFQ35_17035"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HET9932414.1"
                     /translation="MALRKDSSEAGTKASGRSALLPLGELLRELGTSPAGLGSGEATA
                     RLEHDGPNDAASGRSASLRGEWLRTLANPLLGILLFAGVASAILGQHSDVLIIAAIVV
                     SSSALDLWQTTRSRRAVTQLQSRVAPTAKVVRDGGWVVLPRSDVVTGDVVRLSAGDLV
                     PADLRLLQARDLHVHQSALTGESLPVEKTAVVEALSDGGPSSEALLFLGTSVVSGSAT
                     AVVVATGRRTAFGDVISRLGERPDETEFERGMKAFGNLILRTVFVLVLFILVVHLSFG
                     RNALESLLFAVALAVGLTPEFLPLITTVTLAEGALAMARQKVIVKHLASIQNLGSIDI
                     LCSDKTGTITSGEMSLDASLDPLGAPAERPLAFGCLNSAFQTGLASPLDAAILAASRP
                     SPGYEKTDEIPFDFERRRLSVVLRRDAELVMISKGAPEGILDGCTHFEAEGKVEALSE
                     AELARCREAFQRLSQSGFRVLAVAYRAVERPDGHDVHDEREMTLLGYLTFADHLLEGT
                     RESIARLQRDGVSIKILTGDNELVTAHVCRQAGLDVSSMVLGPALEDLDTPALARVAE
                     QNTVFARLSPAGKHRIIRALKSHGHTVGFLGDGINDAPSLHGADVGISVAGAVDVARE
                     ASDIILLESHLDVLHAGIIAGRRAFANVLKYLLMGTSSNFGNMLSMAGAAVVLPFLPM
                     LPTQLLLNNFLYDVSQVTIPSDRVDPALLEKPARWDVRNIQRFMLVIGPVSSVYDFLT
                     FWLLLRAFKFGEVRFRTGWFVESLVTQTLVLFVIRTMDRPWKSKPSALLTVTTLSVVA
                     IAVLLPALPFAPRLGFEPLPVAYLPTLVLLVATYLAIVELIKRRVLVRLLP"
     gene            complement(5758..6117)
                     /locus_tag="VFQ35_17040"
     CDS             complement(5758..6117)
                     /locus_tag="VFQ35_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9932415.1"
                     /translation="MQTPVQITFRGLPRSGPVTAEVHSRVERLEHLFGPLTRCHVVIE
                     VRLHNQRKGNIHSVGIEAVLPGMEIVASRDSGENHDHENVYVAIRDTFNALGREIQSR
                     LKKKRTTTRKSELPRSS"
     gene            complement(6250..>6506)
                     /locus_tag="VFQ35_17045"
     CDS             complement(6250..>6506)
                     /locus_tag="VFQ35_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932416.1"
                     /translation="ARGVACTGNTGEEYALDGFNSIVLQTNDPRELVSMFEWLYSEPD
                     REQLLRTSAQLSAREFLWRHVVERALLPRLSAIEAMTSVI"
BASE COUNT         1123 a         2063 c         2064 g         1256 t
ORIGIN      
        1 atgctcgggc gcggacttac gtgtgttccc ggagcttcgg gaccgaaaga gcttcgtttt
       61 agcttcccct gtgcatgggt tggttagtta gagtgcgttg gtttacttgg ttttcggtcc
      121 gcgaacccag aaaattgggt attcaggctg acatgcgatc ggatgaacga gccccgcacg
      181 ccacgggact tcacacaaaa gcgggttccc cttctgatga acagtggatt ccgaggtagg
      241 cccgacctag aagtgacggg taatcgtggt cgctccggac tttcgcgtcg tcttcgagtc
      301 gcttcctggc tgctatctcg tgctgagccc ggagctgcag atcctcgcgg tcagcgatcc
      361 gtatttgagg gcgacgctca ctacgcgcga gcaactgctc gggcgcggtt tgttcgaagc
      421 gtttccggac aacccggatg acccggaggc gacgggagtt gccaacctaa aggcttcgct
      481 ccagacggtg ttgagtgagc accgcgctca cgaaatgaca atccagaagt acgacatccg
      541 tcggcccgag tcggagggcg gcgggttcga agaacgctac tggaagccgg tgaactcgcc
      601 ggtgttcgat gcgagcggtc gaattcaatg catcgtccac caggtcgaag acgtgacccc
      661 catgatgcgc ctcgagaagc acgggcgcga gcaagatcgg cgcattcaag agctctcgat
      721 ccggggtgaa acgcagcttc ggcagctact cgacgcggcg cccgacgcga tgctagtcgt
      781 ggggtcggac gctcgaatac gcttcatcaa cgcgcaggcc gagcagctat tcggttacgc
      841 gtcgagcgag ctcttgggcc gcgatctcga cttgctcgtc ccttcgaaat tccacgtggc
      901 ccatgtggag cacttgcgcc gcttcttccg cgaaccgcgt gcgcgatcga tgggatcggg
      961 ccttgagctc tacgcgcgcc gcaaagacgg aaaagaagtt cccatcgagg tcagcttgag
     1021 cccgctgctg ctcgaggaag gcgcgttcgt caccgcggcg attcgcgacg tcaccgagcg
     1081 caagcgcatc gaggctgccg ccaatctgct ggcttccagg ctcgcgagcg cggtcgagtc
     1141 gatccaagac gcgttcgcgt tgttcgacga gaccgaccac ctggtgcttt gtaacagcgc
     1201 gtatcgaagg ctggtgaacg aagccatatc ggggcctctc gtggggctca cctacgaaca
     1261 cattctgtcg gcctggctgc cgcagctgga cctcggtgac gaaaagagcc gcgaggcgtt
     1321 cttcgcggag cgacgggctc agcgttcgga gcctcacgtc gatttcaagc tcagaaccgt
     1381 ggacggtcgc agcctgcgcg taatcgacca acgcacgccc gagggcggca tggtcaagac
     1441 catctgggac ttgacggaag acgcccgcat cgcggcggag ctcaaggagg caagagtcgc
     1501 cgcggaggcc gcgagcgcag cgaagagcga gttcttgtcg tcgatgagcc acgagcttcg
     1561 aactccgttg aacgccattt tggggttcgc gcagctgctc tgtcgcgaca aaaagcagcc
     1621 accctcggag cggcagctcg agcgcattcg gcacatcctc gacggcggcg aacatctcct
     1681 gaagctcatc gacgacatcc tcgatctttc tcgcatcgaa gcgggacgcg tctcggtgtc
     1741 aatcgagccc gtcgacgtga acctggttct gcaagagctc cgcaccacgc tcggcccgag
     1801 cgcggcggcg tcggaaatct cactcgtcgt gccgaccgcc gccagccctc ccatcttggc
     1861 cgatcgcacc cgcttcacgc agatcctcat gaacttcggc tccaacgccg taaagtacaa
     1921 tcgccccggc ggtcgcgtga cgttcgaggt gtccgaaccc gcccctgacg tcgtgcgtat
     1981 caccgtcgct gataccgggt tggggattcc gtcgaacaag caaggggcgc tctttcaacc
     2041 gttccagcgc gccgggcaag agaacggccc aatcgaaggc acggggatcg gcctcgccat
     2101 cagcaagcgc ttggccgagg cgatgggcgg gaccgtcggc ttccacagcg agcacggcgt
     2161 gggctcgcgc ttctggatcg agatgccggt tcacgccaca catgctcctt cgagctctcc
     2221 tccgaaaccc cggagggggg gcgagtacgg gctcgaacgc aaggagcaga gcctcgtgct
     2281 ctacgtcgag gacaaccccg cgaacgtgcg gttcatggag gagctcttga gcgcttacga
     2341 gaacctcgag ctcatcgtcg cccgcaccgc agaagaaggc ttggaaatag cgcgcgcgcg
     2401 gaggccccgg gtcatcattc tcgacatcaa ccttccgggg atgagcgggc tcgatgcgct
     2461 gcgtgtgctg cgcgaccggc ccgagaccgc aaggacgccg gtaatcgccc tgagcgcggc
     2521 cgcttcggag ttggatcaaa agcgcggcaa gctcgctggt tttgcgagct atttgacgaa
     2581 accactacgc gtcgacgaat tcgaatcagc gctcagcgct ctattgaatt gagctctgac
     2641 ggagtccgca cgcggacgca cctcctgcgc gcgacgcgca gctcgtgcac tcctcgcgcg
     2701 gggcaaccgc gctgtctgcg atgaggcatt gcggcacacg ccttgctccc tacctcgagt
     2761 ggcggcgtaa cgccgctgag gagcactctc tcatgtctgg cccccttcaa aggatcctcg
     2821 tcgcggtaga cctttcctcg tgttcacgcg ccgtgctcga gttcgcatgt acactggctc
     2881 gtccgatggg atcgacgctc gaagttctgt tcgtgcgtcc ccccgcgcag gtggacgcgg
     2941 ccgaagagct gcatcgtttc gttcatccca tcgtcaaaga cgccgggctg acggtcatcg
     3001 aacgcgtcga ggcgggagac gctcgcgagc gtatcgtggc gatcgctgcg gaagagaagt
     3061 tcgacctcgt cgcggtcggt acccacggtc gtacgggccg cccccgctca ctcgcgggca
     3121 gcgtggcaga gagcgtcgtg cggaccgcga cgtgcccggt gatgacggtt cgcgagcgct
     3181 gaagtggcgc ttcgcaagga ctcctccgag gcaggaacca aagcgtcggg gcgctcggct
     3241 ttgctgccac tcggagagtt gctgcgcgaa ctcggtactt cacctgccgg cctgggttcg
     3301 ggtgaagcga ccgcgcgcct cgagcacgac ggcccgaatg acgccgcttc ggggcgctca
     3361 gcgtcactgc gaggcgagtg gttgcgcacg ctggccaatc cgttgctcgg gatcttgctc
     3421 ttcgctggcg tcgcgtcggc gatcttgggt caacacagcg acgttctcat catcgccgcg
     3481 atcgtcgtct cgagctcggc gttggatctg tggcaaacga cccgttcccg ccgcgccgtc
     3541 actcagctgc agtcgcgcgt ggcgccgacg gcgaaggtcg tccgcgacgg gggttgggtc
     3601 gtgctcccgc gctccgatgt cgtgacgggg gacgtggttc gactctcggc tggcgacctc
     3661 gttcccgcgg atttgcgcct actgcaagcg cgtgaccttc acgttcatca gtcggcgctc
     3721 acgggcgaat cgctgccggt cgaaaagacg gccgtcgtcg aggcgctcag cgacggcgga
     3781 ccgagttcgg aggcgctcct cttcctcggg acctcggtgg ttagcggcag cgcgaccgcg
     3841 gtggtggtcg caaccgggcg ccgcaccgcg ttcggagacg taatatcgcg gctcggcgag
     3901 cgccccgacg agactgagtt cgagcgcggt atgaaggcgt tcggcaacct gatcctgcgc
     3961 accgtcttcg tgttggtgtt gttcatcctc gtcgtgcact tgagcttcgg tcgaaacgcg
     4021 ctcgagtcgt tgttgttcgc ggtcgcgctc gcggtgggcc tcacccccga gttcttgccg
     4081 ctgatcacca ccgttactct ggccgaggga gccctcgcca tggctcggca gaaggtgatc
     4141 gtgaagcact tggcttccat tcagaacctc ggaagcatcg atatcctgtg cagcgacaaa
     4201 acgggcacga tcacgagtgg agagatgtct ctggacgcgt cgctcgaccc cctgggcgcg
     4261 cccgccgagc gtccgctcgc gttcgggtgt ctgaacagcg cgtttcaaac cggcctcgcg
     4321 agcccgttgg atgcggccat tctagccgcg agtcgcccgt ctcccggata cgaaaagacg
     4381 gacgaaatcc cgttcgactt cgagcgccgg cggctctcgg tagtgctacg acgggacgcc
     4441 gaactcgtca tgataagcaa gggagcgccc gaggggatct tggacggctg cactcacttc
     4501 gaggccgagg gcaaggtcga agcgctcagt gaggcggagc tcgcgcggtg tcgcgaagcg
     4561 ttccaacgtc tcagtcagag cggctttcga gtgctcgcgg tggcgtaccg agccgtcgag
     4621 cgtcccgacg gtcacgacgt ccacgacgag cgtgagatga cattgctcgg ttatcttacg
     4681 ttcgcggacc atctgctcga gggcacgcgc gagtccatcg cgcgcctcca acgcgacggt
     4741 gtttcgatca aaatcctgac cggcgacaac gagctcgtca ccgcgcacgt gtgtcggcaa
     4801 gccgggctcg acgtgagttc gatggtcctc ggtcctgcgc tcgaagatct cgacacgccc
     4861 gcgctcgccc gcgtcgccga gcaaaacacg gtttttgcgc gcctctcgcc cgccggtaag
     4921 caccgcatca tccgcgccct caaatcgcac gggcacacgg tcgggttctt gggagacggc
     4981 atcaacgacg ccccttcgct gcacggggcg gatgtgggaa tctcggtcgc gggcgcagtc
     5041 gacgtggccc gcgaagcctc cgacatcatc ttgctcgaga gtcacctcga cgtactgcac
     5101 gcggggatca tcgccggtag gcgcgccttc gcgaacgtcc tcaagtacct gctcatgggc
     5161 acgagctcca atttcggcaa catgctcagc atggcggggg cggcggtggt tctgcccttc
     5221 ctgccgatgc ttccgacgca gctcctgctc aacaacttcc tctacgacgt gtcgcaggtg
     5281 acgattccgt ccgaccgagt cgacccggcc ttgctcgaaa agccggcgcg ttgggacgtg
     5341 cgcaacattc agcgcttcat gctggtgata ggtccggtga gctccgtgta cgactttctg
     5401 acgttttggc tgctcttgcg cgctttcaag ttcggggaag tgcgcttccg aactggctgg
     5461 ttcgtggagt cattggtcac ccaaacgctc gtgctcttcg tgatccgcac catggaccgg
     5521 ccttggaaga gtaagccgag cgcattgctg acggtgacta ccctctcggt ggtagccatt
     5581 gccgtgctgc tcccggctct tccctttgct ccgcgactcg gcttcgagcc gttgccggtc
     5641 gcatatcttc ccaccctggt gttgctcgtg gcgacgtatc tcgcgatcgt cgagctcatc
     5701 aagcgccgcg tcttggtccg tttgcttccg tgagtcgttt ccagcgagcc cgcggtctca
     5761 ggaagagcgg ggtaactccg actttctcgt cgtcgtgcgc ttcttcttca gtcgactctg
     5821 gatctcgcgg ccgagcgcgt tgaacgtgtc acggatagcg acgtacacgt tctcgtgatc
     5881 gtggttttcc ccagagtcgc ggctggcgac gatttccatg ccgggcagca ccgcctcgat
     5941 gcccacgctg tggatgttgc ctttgcgttg gttgtgaagg cgaacctcga ttacgacgtg
     6001 gcatcgcgtc aaaggtccaa acaaatgctc gagtcgctcg acccgcgaat gaacctcggc
     6061 ggtgaccggt cccgagcggg gtaagcctcg aaacgtgatt tgcaccggag tctgcatggc
     6121 tccgggagac ccgaaatgca ttgcgcgtgc cgtcccgaaa aaaacagcgt ttctcgcggg
     6181 cagtgagcca agacgcgaac gcgctgcgtg cgagctagct cagtcacgaa atttgtgcgc
     6241 tcagcggacc tatatgacag atgtcatcgc ttcgatcgca gacagacgcg gcaacagcgc
     6301 gcgttcgacg acgtgcctcc ataggaattc tctcgcgctg agctgcgccg aggtccgcag
     6361 cagttgctcg cggtcgggct ccgaatagag ccactcgaac atgctgacga gctccctcgg
     6421 atcgttggtt tgcaacacga tggagttgaa tccatccaag gcgtactcct cgcccgtatt
     6481 gccagtgcac gcgacgcctc tcgccg
//
LOCUS       DASTKS010000827         4839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1248455, whole genome shotgun sequence.
ACCESSION   DASTKS010000827 DASTKS010000000
VERSION     DASTKS010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248455"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VFQ35_17050"
     CDS             <1..1383
                     /locus_tag="VFQ35_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9932417.1"
                     /translation="LKKERDKASVARLDDLAREVAELSGERDRMRAQWMREKESIVRT
                     REKQTKLEELRAEEERARRAGDLGKAAEIHYGKIPEIERAIEADRAELNEIQKNGAYL
                     KEEVGEQDIAAIVSKWTGVPVSKMLEGEMQKLLNMENSLRERVVGQDAALAAVANAVR
                     RSRAGLGDSRRPIGSFLFLGPTGVGKTETARALAEFLFDDERAMVRIDMSEYGERHAV
                     SRLFGAPPGYVGYEEGGQLTEPVRRRPYSVLLFDEIEKAHPDVFNALLQLLDDGRLTD
                     GQGRTVDFKNTVVILTSNIGSSELAAFEERRDLSDHERNELFQTVAMNAVKQHFRPEF
                     VNRLDEIVVYHRLGREQLRRIVDIQLSSLRERLGQRELLLEVSDSAKEFLGEVGWDPQ
                     FGARPLKRAIQRHVEDALAKRVLAGEFLPGDTIVVDRGASGELSFTRRPAPSDGKPAP
                     RGNGSATAHA"
     gene            1467..1643
                     /locus_tag="VFQ35_17055"
     CDS             1467..1643
                     /locus_tag="VFQ35_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932418.1"
                     /translation="MLSRFVLYAALLLLGWKLLARVRFRELGRRLDKIVNATLVAIVV
                     VYSAELLYIWLAAK"
     gene            complement(1646..3292)
                     /locus_tag="VFQ35_17060"
     CDS             complement(1646..3292)
                     /locus_tag="VFQ35_17060"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9932419.1"
                     /translation="MADTDQAPPAPTEAGLPTKIGGFRVDRRLGQGASAEVFLATHEV
                     TGQVVALKVLRKELDSSVARQVRTRFLLEERIAATIPDPRIVKVYETSKPGEEPYFIA
                     MEYLEADSFCDYFKRRRELGSVASQAYLNELCRLSYEVSKAMSAAHARGVVHRDLKPD
                     NVLVLRREARHSSERVKIVDFGIAKAPIGVLTGVAATFTPHHTELGTVMGSPPYMSPE
                     QYGRAHAVTGKSDVFALGIMLTISAVGLDHDSLEVNRSCWTLPDDFERALTLGPPLPE
                     RLEQLLRSMVAESPEDRPEMREVALELGRLSQPNEAVAEAVFAYASKRRIPPKKELIR
                     LVSELEDQRDLTDDENEFVRQAPAAMLASDKPWLRYAVVTGLASSSAALAALYFRDHD
                     DLRRATARAEAELDRVRGDATALRRDLEAEAQHRKVLLARATTQEQMLESLDKSQGEL
                     KREARVRESRLTQCRAVSDELKECTDQRDLCELGASERKAEVTRLKTEVETLRRDADA
                     AVRCQHELESKAGELTETANRLRLCSDGLRGKPSDAPGGS"
     gene            complement(3472..4719)
                     /locus_tag="VFQ35_17065"
     CDS             complement(3472..4719)
                     /locus_tag="VFQ35_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044967075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9932420.1"
                     /translation="MATACVLCIGTELTRGEVVNSNASFLADGLTAAGFEVTTIETVD
                     DHEGRIIDSLRRLSAGHAVIVATGGLGPTTDDITTACVAKLLDVPLERDAASLAAIKA
                     RFERLGRHMSPSNQKQADFPKGAIVLPNPNGTAPGFSVQLGNAYAAFMPGVPREMGPM
                     FRDSIVPAIRHLVDEALYQVRLRTFGLPESEVNDRLAGVEETFGVIVGYRASFPTIEV
                     KLLARDAKSSEAEARARAAAAEVRKRLGEDIVFAEGDVTFAEALGRELVARHLTLAAA
                     ESCTGGLVGQLVTARAGSSGFFLGSAVTYANEAKMKLLGVGEELLIEHGAVSAEVARA
                     MAEGARRLFAADVTLGITGIAGPDGGTAAKPVGLVHYAVTTEHGTVDRSFVFPGDRDM
                     VRLRAAYAALSLAYQRVRAGDAG"
BASE COUNT          795 a         1580 c         1631 g          833 t
ORIGIN      
        1 ctcaagaagg agcgcgacaa agccagcgtc gctcggctcg acgatctcgc gcgtgaagtc
       61 gcagagcttt ccggcgaacg cgaccggatg cgcgcccagt ggatgcgcga gaaggagagc
      121 atcgtccgca ctcgagagaa gcagacgaag ctcgaagagc ttcgcgcgga agaagagcga
      181 gcgcggcgtg ccggagacct cggcaaggcg gctgaaattc actacgggaa aatccccgag
      241 atcgagcgcg cgatcgaggc ggatcgagcc gagctgaacg agatccagaa gaacggcgcg
      301 tacctcaagg aagaggtcgg cgagcaggac atcgcggcca tcgtttcgaa gtggaccggt
      361 gtgcccgtct ccaagatgct cgagggcgaa atgcagaagc tcttgaacat ggagaacagc
      421 ctgcgcgagc gcgtggtcgg gcaagacgct gctctcgcgg ccgtcgcgaa cgcggttcgt
      481 cgctcacgcg cagggctcgg cgactcacgc aggccgatcg ggagcttctt gttcctggga
      541 ccgaccggcg tcggtaagac cgaaacggcg cgcgccctcg ccgagttctt gttcgacgac
      601 gagcgggcga tggtccgcat cgacatgagc gagtacggcg agcgccacgc ggtttcgcgg
      661 ctgttcggcg ctcctcccgg atacgtcggt tacgaagagg gcgggcagct caccgagccg
      721 gtgcggcgcc gtccttacag cgtgctgctc ttcgacgaga tcgagaaggc gcaccccgac
      781 gtattcaacg cgctcttgca gctgctcgac gatggtcgcc tgaccgacgg tcaggggcgt
      841 accgtggact tcaagaacac ggtggtcatc ctcaccagca acatcggctc gagcgagctc
      901 gccgccttcg aagagcggcg cgatctgagc gatcacgaac ggaacgagct gttccagacc
      961 gtcgcgatga acgcggtgaa gcaacacttc cgccccgagt tcgtgaaccg cctcgacgag
     1021 atcgtcgtct atcatcgcct cggccgtgag cagctgcggc gcatcgtcga catccagctt
     1081 tcgtcgctgc gtgagcgcct cggccagcga gaactcctgc tcgaggtcag cgactccgcc
     1141 aaggagttcc tgggcgaagt cggctgggat ccacagtttg gcgcccgccc gttgaagcgc
     1201 gccatccagc ggcacgtcga ggacgcgctc gccaaacgag tgctcgcggg cgaatttttg
     1261 cccggcgaca ccatcgtggt cgaccgcggc gcgagcggag agctctcgtt cacgcggcgt
     1321 ccggcgcctt cggatggcaa acccgcgccg cgcggcaacg gttcggcgac ggcgcatgct
     1381 tgatgggttg gttttcgctg cgcggcaagg ctcagcgccg cgcgcctgat gggttgcgga
     1441 cgcgcggcgc ttgggcatga tgaagcgtgc tgtcacgctt cgtgctctac gccgcgcttc
     1501 tgctcctcgg ctggaagctg ctcgcgcggg tgcggttccg cgagctcggt cgccggctcg
     1561 acaaaatcgt gaacgcgacg ctcgtcgcca tcgtcgtcgt ctactccgcc gagcttctct
     1621 acatctggct cgcggcgaag tgatttcagc tgccgccggg cgcgtcgctc ggcttgccgc
     1681 gtaggccgtc gctgcacaac cgaaggcgat tcgccgtctc ggtgagctcg ccggccttcg
     1741 actcgagctc gtgctgacaa cgcacggccg cgtctgcgtc gcgccggagc gtctcgacct
     1801 ccgtcttcag ccgggtgacc tctgccttac gctcgctcgc gccgagctcg cacaaatcgc
     1861 gctggtccgt gcactccttg agctcgtcgc tgacggcgcg gcactgagtc aggcggcttt
     1921 cgcgaacgcg cgcctcgcgc ttgagctcgc cttggctctt gtcgagcgat tcgagcattt
     1981 gctcttgggt ggtggcgcgc gccagcaaga cctttcgatg ctgggcttcg gcctcgaggt
     2041 cgcgtcgtag tgcggttgcg tcgccccgca cacggtcgag ctctgcttcg gcgcgagcgg
     2101 tcgctcgtcg cagatcgtcg tggtcgcgga aatagagcgc ggcgagcgca gcgctcgacg
     2161 atgcgaggcc cgtcaccacc gcgtaccgga gccagggctt gtcgctcgcg agcatcgcgg
     2221 ccggggcctg gcgcacgaac tcgttctcgt cgtcagtgag gtcgcgctgg tcctcgagct
     2281 cgctgaccag acgaatcagc tcctttttcg ggggaatgcg gcgcttgctg gcgtaggcaa
     2341 acacggcttc ggcgaccgct tcgttcggct gcgacaggcg gccgagctcg agcgcgacct
     2401 cgcgcatttc ggggcgatcc tcgggtgact cggcgaccat gctacgcagg agttgctcga
     2461 gcctttccgg cagcggcgga ccgagcgtga gcgcgcgctc gaagtcgtcg ggcagagtcc
     2521 agcacgagcg attcacctcc agtgagtcgt ggtcgagacc caccgcggaa atggtcagca
     2581 tgatgccgag cgcgaacacg tcggacttcc cggtgacggc gtgagcgcga ccgtactgct
     2641 cgggagacat gtagggaggc gaacccatga ccgtgccgag ctcggtatgg tgcggcgtga
     2701 acgtcgccgc gacgccggtg agcacaccga tgggagcctt ggcgatgccg aaatcgacta
     2761 ttttcacgcg ctcgctcgag tgccgcgctt cacggcgcaa cacgagcacg ttgtcgggct
     2821 tgagatcccg atgcacgacg ccgcgggcgt gagcggcgga catcgccttg ctcacttcgt
     2881 agctcaagcg acagagctcg ttcaggtacg cttggcttgc gacgctgccg agctcacgcc
     2941 ggcgcttgaa gtagtcgcaa aacgagtccg cctcgaggta ctccatcgcg atgaagtagg
     3001 gctcctcgcc gggcttgctc gtctcgtaga ctttcacgat gcgcggatcg ggaatggtcg
     3061 ccgcgatgcg ctcctccaac agaaaacgcg tgcggacttg tcgcgccacg ctcgagtcga
     3121 gctctttgcg caacactttc agtgcgacga cctgacccgt cacttcgtgc gttgcgagga
     3181 aaacctccgc cgaagcgccc tggccgaggc gtcgatcgac gcggaaaccg ccaattttgg
     3241 tggggagccc ggcttcggtc ggcgcgggcg gtgcctggtc ggtgtcggcc atgggaggtc
     3301 tcgaatcgaa gggtaccgaa ctgggctgcg cgggaccaag aactgcgctc tgcccacccc
     3361 aaaaagatcc gagaatttgg cccgccctcg aaatttcgac tagcgccccc ggttcgggat
     3421 acgcgcaatc atcgcgggcg gtgacggtgc cgcatcgttg tcggcgctga tctagccagc
     3481 gtcgcccgcg cgaacacgtt ggtacgcgag gctgagcgcc gcgtacgccg cgcgcaagcg
     3541 gaccatgtct cggtcgccgg ggaagacgaa gctcctgtct accgtgccgt gttcagtagt
     3601 gaccgcgtag tgaacgagcc cgactggctt cgctgcggtc cctccgtcgg gccctgcgat
     3661 gcccgtgatg ccgagcgtca cgtccgccgc gaataggcgg cgcgccccct ctgccatcgc
     3721 tcgcgcgacc tcggcgctga cggcgccgtg ctcgatgagt agctcctccc cgacgccgag
     3781 gagcttcatt ttggcttcgt tcgcgtacgt gacggcgctg cccaagaaga agccgctcga
     3841 gccggcgcgc gccgtgacga gttggcccac gaggccgccc gtgcacgact cggcggcggc
     3901 gagagtgagg tgacgcgcga cgagctcgcg gcccagggcc tcggcgaagg tgacgtctcc
     3961 ctctgcgaac acgatgtcct cgccgaggcg cttccgtacc tccgcggccg cggcccgcgc
     4021 ccgcgcttct gcctcgctcg acttggcgtc gcgggcgagc aacttcacct cgatggtcgg
     4081 aaagctcgcg cgataaccaa cgatgactcc aaacgtctct tccacgccag ccaggcgatc
     4141 gttcacttcg ctctcgggca ggccgaaggt acgcaagcgc acttggtaga gcgcctcgtc
     4201 caccaaatgg cgaatggcgg gcacgatgct gtcgcggaac atcggcccca tctcgcgcgg
     4261 aacaccgggc atgaacgctg cgtaagcgtt tccgagctga accgaaaaac cgggggcggt
     4321 gccgttcggg ttcggcagca cgatggctcc ctttgggaaa tccgcctgtt tttggttgct
     4381 cggcgacatg tggcggccga gtcgctcgaa gcgcgctttg atggccgcga gagaggctgc
     4441 gtcgcgctcg agcgggacgt cgaggagctt ggcgacgcaa gcggtcgtga tgtcgtccgt
     4501 agtcgggccg aggccgccgg tcgcaacgat cacagcgtgc ccagcgctga gccgtcgcag
     4561 actgtcgatg atgcggccct cgtggtcgtc gaccgtctcg atggtggtga cctcgaagcc
     4621 cgccgcggtg agcccgtccg ccaagaagct cgcgttgctg ttgacgacct cgccccgtgt
     4681 cagttcggtc ccgatgcaaa gcacacacgc cgtggccatg ggacaagagt ttcacggcag
     4741 cgcgcgagcg tcgaggctct cgacgccggc tctcgtgcgt ggaagccttc gagcgcgagc
     4801 agcctcgccc catgcagatt caatcgaagc tcccaacgg
//
LOCUS       DASTKS010000828        15599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1694769, whole genome shotgun sequence.
ACCESSION   DASTKS010000828 DASTKS010000000
VERSION     DASTKS010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694769"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1275)
                     /locus_tag="VFQ35_17070"
     CDS             complement(79..1275)
                     /locus_tag="VFQ35_17070"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9932421.1"
                     /translation="MASLAGLPLAHAQAPAGDKPAAAPAKPPAPTPAPAAPAAPGAAA
                     AAPDAKKADPKSAAPAAAPKSAAPKADATKAGAATGVGATIGAGAATAAKPKEPDKKT
                     RDAARKAYGEGEKAFNSGDFPGALEGFRKADALIPSPHAQYWTAKSLDKLDRVEESIA
                     AYQRYLENPDANRAGAEKVTDAENRVSELRLKLIAQVEVVTVPAGASVSVDGNPEPGV
                     TPLALKLAPGAHKITLSATGYEPKEINLDAKAGDKIEQRHELALKPPPAPPPAPIAEA
                     PATPPPPPPPPEKRSMVPAFVTLGIAGASAIVGTVFGVQALSARSKFKDKPTTSNADD
                     TERNALISDMAFGVAITLGVTGVVLLTSGDEPQETARTTPPKLKLEFDGYASKQAGGA
                     SARLRF"
     gene            1478..8623
                     /locus_tag="VFQ35_17075"
     CDS             1478..8623
                     /locus_tag="VFQ35_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932422.1"
                     /translation="MDSSQTPSFQESDVADAAADDLLSEAAPGKRALQAAPEPPDSGP
                     RSAASLALDRYALGEPVARPSGGRAELQVLLASIAAAGQARDEHQERSATAALARALW
                     QRGFELDQAIKLARRSLLLGDDPELREELSSWFSNLGEAALAAATLRPLLASATPEQA
                     SALLLRMSVLLCRAGEARAARDVLEQAMRQAPQDPQPTELCAGIVGFAPEAVSPEEAA
                     QFYLEAADRRTRLGERAAAFENMLRAFEAAPASALACDALARVLTERGRTSAADEVRR
                     EHARHVGAQSRAVHLRRLRDALAARDLPRALGACLDARLDAEIELKNAIGALSSRDAA
                     NSADPLFGFDDLLYRLGLFDLLAARIELGSERLSGAEGARARVALGRIYFGPLARPDR
                     AVEVFVDALLCDPGNEDALRALLEHARATYDYSPLVEALVRIVESGTSLPVRRACLSR
                     LEKLAETQLNDDGLRLWVEMQRSLLAPEDQAIAERLERLRAVEEHLAAALAGARAELA
                     SQTATDRIPTLRRMASLLFSRPDRADALLPVLLELVELAPEERGFQLAVERVLTRQGR
                     FEELEAFYLRALGHTTLGAERARLSWALSSVRRRRGDIEGALGILAPILDEDGAFPAC
                     ISMIVALSAQSGEELLRARALLRLGASLPPPLRSVLCAVAAEILLRKSELTAARSAAE
                     QAVVADSSSARALVTRGAVGAVLRDQAGIEAMERALAVAVPRAELCEALASAYDAIEQ
                     QEVSFAYCLRRIALRPSDPVAAADRLTRATHSDHAARLADTLAWLLGSPQPLESLTPL
                     LAAALRRLSTLEPPRAATLAKRALDVMGPRSSELRLAVLAVADLTGERALAITTLERW
                     LASGAPSADRAELLLDVSRRRRALTDADGAARALYRAIREGAFPISVLAELDVALPAK
                     SPDGELALLVSRAEALSALSEADARSTARAWRELGAAYHELGQDRPAAIAAWERAFAL
                     DPDRGIENFAADLLAFDGPELAVAELTRLSAHRSERTQACRLLSVAAAIALEAGNHSS
                     ALSLAERALSLDPSRTDVLAVAERAAGPADLDILEKLYDELGLAALGQFGARAVHYRA
                     ARQLEKRGELARSFRHAVRAFEAVPSEGAAYLAMVRLADAVGETTEAVRALQAASEAC
                     NNPELGSAFLERAALLTGPGVEGQRQRVDVLLRSLVVRPELHTVSALGEAVQALIRLA
                     PEERDVLELRVRRALGSLLGHVQDGEGARIAISFAVWVLRVFNAAGSAVQALARAVEC
                     QPTLTDYAELTPHAAVLAPEAGPWLASALERAKTVSFSSELQRLLLEIADARGDRAQI
                     AELAVSLAEANPDDVELVARAERAARAAGEPSLIARALDVVPPAERQRYLELSADEAQ
                     RRGDLPRAIELIEKARSLPNITHEASRGMFEHLIELHEQLADADALERLLQAEIERET
                     GDLARRARLRHKLALVLSEVGERERSLVEIESAAGDLPSEPALIEDYVRLSRDAFDAM
                     RLISALGLGVRLQPQSTHQLRWLRELASLLEQQGDFGLACEQWAKVLELDPKDDAAAS
                     AMERDAARRGDYEEVVKWLARRAENTASHADLRSIRLRRAMVLEQRLGRPDEARAELE
                     LLTSVIGDNLSVLRVLADLNERLGAPLKAAPVWLRGSAVTKNRDEAEDLACRACEAYL
                     ASGEVEAAERVLEGMRAWANSRRMLSLAVEVERRRNHPLALADALDDLASLDESPTEQ
                     RSRWLVEAAQISFMAGQDQLARARAERAAQLSPGFAEAVLLACSLEYQHREPTSPDQA
                     LRIIERLEPLSVASDEQAELRAFLLAAALESAGRPVAAGLALEQAERSLGKRPLLAWA
                     LAERLTRGGETERALAYYDAALGGDLRGLGQRSRVALRAAHAARDIGASERAVGYLEL
                     AAEDPDQRDTARAEIQALLSENRGFGAKLSGLDLPATEAPVVSLGRYSQKPPADVAEE
                     RIVVPPIRSAPPRSGEPAAIKGVALGRIFDAQEPTPTTDPPEEVISSDFSSTRVAKFE
                     PDHAELYKDLSSGSFEAGRKLIAELEQRSDRSHDLVMVCRKLVFLNPTELWALRSLRE
                     AALADKNLAYARAIEHVLGVLEPDDPWVEPPALEDQPREPDAVQALLTRVGHGSPALE
                     ALALVWEGAERVFRHDPSAYGVTGRERIPHGAPAPLGRMYGCIASALGMLRAPLFQRR
                     SDGGPVNVSLALVSPPAVLVTGEVRKETPELRFHLGAMLLACLPQFVLLVGSPEPEAR
                     AILRGLSFAFGPPQLGSISASVPNLAEVLWQSIPPRMQRRLRELGSDALAFEYDAVIQ
                     SARVAIRRAGLFACGDLGVALREAGFEDGIDGGPGESLRTIAESWPRNPSIRSLLLLA
                     TSAEYAQVRFQPGRGPR"
     gene            8929..9447
                     /locus_tag="VFQ35_17080"
     CDS             8929..9447
                     /locus_tag="VFQ35_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932423.1"
                     /translation="MSACIVAPPAEYEESTRTPPIFDLVRAKPFIGDIIVVDQDAGLE
                     SARKIQFNVPVRSEDQGEEIIGAVHIDYGSGQPRDTFTKSQQKLPASTLDDVSRVWDQ
                     NVTFFGGLEPGCHQVTLIATHRSNWDDERFRPIVGRAEYDTAIAMWWMIYNPNRPPTS
                     PVTCPSGAEIER"
     gene            9444..11435
                     /locus_tag="VFQ35_17085"
     CDS             9444..11435
                     /locus_tag="VFQ35_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9932424.1"
                     /translation="MIDHRRARAFSVLLASLVGSTGCSLAGLEERELPYSRNTCENQG
                     DCPESTTCQSSAGSSRCVSTIGSFLTLLFRVSPAVSASNLTGQELFQLATDVPAVANR
                     YDLELSDLTELSGAVTASIPAGCGPAWGLPASGTGGSSGTGGSSGSTGTEGGAAGSSS
                     GGANTNPGSAGTGAGGGATERSRIEARVTFVPSERRWGLATESHVTQTTRTSPFRALL
                     SAGSYDVYIEPKSVPSNPGEAGANDCSLTPRLLRGTKLSSGNTSLMLDLPQPTRLQLE
                     VRVMGTDSLEGFSVDLLEPYSGRVVSTRRQLSAPTRTEGGIRVYTVQVNYSPTANLAS
                     DVVKSELVRLSPPAGVTAPALYFERTALELFTKGSATIELATSLPNTLSFEGQVTDAQ
                     DRPQVSAVSLVATSLDRLPAGTLGGFVRSMKTDDQGRFRAELLPGTYQVTASPVSSRA
                     DAPLATRRETWVIAGKPVQQAGQTIVLRPAPRVRGRLLLPDRDQGAAGVTLTAEASPL
                     SLSPTILERELGQVSAVPSILSSLVTPPDGVFELFAESGTYDFSARPPDGAGFPWLVL
                     PGLRIPEGHSEVVLEDLHLPWPVPLEISTSLKGREDSAALLLGAHVQAYALLDAQGRP
                     TADPGLAEVAVPVADARVDERGIATLLLPARLNDPVSTP"
     gene            11857..13104
                     /locus_tag="VFQ35_17090"
     CDS             11857..13104
                     /locus_tag="VFQ35_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932425.1"
                     /translation="MLPGDDCAQDLAGALHEVSNALTVVLGWIDVAHAKLPPGPEREA
                     LEVARTHARLGYRIARQSIGAEVAEPASTQGSAQSVARVAMTGVTPQAQRAGVRLVFD
                     CDNPDAHTRVSDVGEALQILTNLLLNAIAFTPRGRSVTLSVREDENNAVFRVVDEGPG
                     LPPERARNVFQGPSSTRRGGAGIGLRHSKSLAEQHGGSLNVEVLPSPTLPRRPPTPPS
                     ARRSAPDLHASGIVPVSSATPPSISPPNCSEPRSGGACFELRWPIAEARSSTRPQQPV
                     GASIKGARVLVVEDDAAVRSLIELSLDARGVETVLVQSAAELEQALGAAPYDVALLDM
                     SPIAEHAPELLQRLRQSSPGIALILISGLASRVPEAIERDVAAWVRKPFEMGEVIDVM
                     SRLLAERPPASCPPTRRPSTATG"
     gene            13404..13487
                     /locus_tag="VFQ35_17095"
     tRNA            13404..13487
                     /locus_tag="VFQ35_17095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13438..13440,aa:Leu,seq:gag)
     gene            complement(14492..14758)
                     /locus_tag="VFQ35_17100"
     CDS             complement(14492..14758)
                     /locus_tag="VFQ35_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932426.1"
                     /translation="MNETLKVASNAILAVLVQAKPANTTSAPSQPGYGARGGPDVIHW
                     TELDAQFARKIQGRSVRPLSQKTPEEKALLDQMRAIQNKKEKRE"
     gene            15296..>15599
                     /locus_tag="VFQ35_17105"
     CDS             15296..>15599
                     /locus_tag="VFQ35_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932427.1"
                     /translation="MRPSAVCGRGGDGLAAVHESTILRPCALQYDASASDEALKRVVV
                     VKYFPRVPNIYINADGYLKGFLGLTWFPSGDAKAHVAQYERQVAAVLFNIWGRRVGS"
BASE COUNT         2451 a         5332 c         5058 g         2758 t
ORIGIN      
        1 tcgcgaattt tcgagacagc ctcccgggct tggttgcggc ctcgcagaac tacgggccgc
       61 aaccttcggg ccgctggctc agaagcgcag ccgggccgac gcgccgccag cttgtttgct
      121 ggcgtagccg tcgaactcga gcttgagctt gggcggcgtc gtgcgcgcag tctcctgcgg
      181 ctcgtccccg ctcgtgagca gcaccacacc ggtgacgccg agcgtgatcg cgaccccgaa
      241 cgccatgtcg gaaatcaggg cgttgcgctc ggtatcatcg gcgttggacg tggtcggctt
      301 atctttgaat ttgctgcgcg cactgagcgc ctgcactcca aacaccgtgc ccacgatggc
      361 gctcgcgcct gcgataccca gcgtcacgaa cgccggcacc atgctgcgtt tttcgggggg
      421 tggcggtggc ggtggcgggg tcgccggcgc ctcggcgatc ggcgcgggcg gcggagccgg
      481 gggcggcttt agcgcgagtt cgtgccgttg ctcgatcttg tcgccggcct tggcatcgag
      541 attgatctct ttgggctcgt acccggtggc gctgagcgtg atcttgtgcg cgccgggggc
      601 gagcttcaga gccagcggcg tgacgcccgg ttcgggattg ccatcgaccg aaacactggc
      661 acccgctggg accgtgacga cctccacctg agcgatgagc ttgagccgga gctcgctcac
      721 gcgattctct gcatcggtga ctttctccgc gccagcccgg ttcgcgtcgg ggttctcgag
      781 gtagcgctga taggcggcga tgctttcttc cacgcggtcg agcttgtcca agctcttcgc
      841 cgtccagtac tgcgcgtggg gcgacggaat caatgcgtcg gctttgcgga agccttcgag
      901 cgcaccgggg aaatcacccg agttgaaggc cttttcgccc tccccgtacg ccttacgcgc
      961 ggcgtcgcgc gtcttcttgt cgggttcttt gggtttcgcc gccgtggccg cgcccgcacc
     1021 aatcgtcgcg cccacgcctg tggccgcgcc cgccttcgta gcatcggctt tcggagctgc
     1081 agacttcgga gccgcagcgg gggcggcgct cttggggtcc gctttctttg cgtccggtgc
     1141 ggccgccgcc gcgcctgggg cagcgggcgc agccggtgcc ggtgtcggcg cgggtggttt
     1201 cgcaggcgcg gccgcaggct tgtcacctgc aggtgcctgc gcgtgagcga ggggaagccc
     1261 tgctagcgaa gccacgctga gcgtggtgct tagggccgtg aggcggaata aatagcgaaa
     1321 acgagagtca tgcatgcaag ccgagcctcc gcgaccgccc gcaagccggg cgtccgcgct
     1381 caggctagtc cgctactaag tccggggaca agcacgactt tcgctatagt gtatcgactg
     1441 cccttcgccg gcacattgcg ggcgcgggca tccggtcatg gactcctcgc aaaccccaag
     1501 ctttcaagag tccgacgtgg ctgacgcggc ggccgacgat ctcctgagcg aagctgctcc
     1561 tggtaaacgc gcgctacagg ccgcgccgga gccacccgac tcagggccgc ggtccgccgc
     1621 ttctctcgcg ctggatcgct acgcgctcgg tgaacccgtg gcccgcccga gcggtggtcg
     1681 cgccgagctg caagtgctcc ttgccagcat cgcggccgcc ggccaggcgc gcgacgagca
     1741 tcaggaacgc agcgccaccg ccgccctcgc gcgcgcgctt tggcagcgcg gtttcgagtt
     1801 ggatcaagcc atcaaactcg ctcgacgctc gctactactc ggcgacgatc ccgagctgcg
     1861 cgaggagctt tcttcctggt tctcgaacct cggggaggcg gcgctcgcgg cggccacgtt
     1921 gcgcccgttg ttggcttcgg ccacgccgga gcaggcgtcc gccctgctgc tccgcatgtc
     1981 cgtgctgttg tgccgcgcgg gagaagctcg agcggctcgc gacgtgctcg aacaagccat
     2041 gcggcaggcg ccgcaggatc cgcagcccac cgagctctgc gcgggcatcg tcggttttgc
     2101 acccgaggcc gtatcgcccg aggaagccgc gcaattctac ttggaggcgg ccgatcgtcg
     2161 cacgcgtctc ggcgaacggg ctgcggcgtt cgagaacatg ctgcgcgcct tcgaggccgc
     2221 acccgcgtca gcactggcct gcgatgcgct ggcccgcgtg ctcaccgagc ggggccgcac
     2281 gagcgcagcg gatgaggtgc ggcgtgagca cgcccgacac gtcggcgcgc agagtcgagc
     2341 cgtccacttg cgtcggctgc gtgacgcgct ggcggcgcgc gatttgcccc gcgcgctcgg
     2401 ggcctgtctg gacgcgcgtc tggacgcgga aatcgagctc aagaacgcga tcggggcgtt
     2461 gtcatctcga gacgccgcca atagcgccga cccgctcttt gggttcgacg atttgctgta
     2521 tcgcctcggc ctattcgact tgctggcggc gcgcatagag ctcggtagtg agcgactgag
     2581 cggggccgag ggcgcgcgcg cccgggtcgc tctcgggagg atctacttcg ggccgctcgc
     2641 gcggcccgat cgcgcggtcg aggtcttcgt ggacgcgctg ctctgcgatc ccggcaacga
     2701 agacgccttg cgcgcgctgc tggagcacgc gcgtgcgacg tacgactatt ctccgctcgt
     2761 cgaagctttg gtgcgcatcg tcgaaagtgg cacctcgctg ccggtacggc gggcgtgtct
     2821 ctcgcgcctc gaaaagctcg ccgaaaccca gctcaatgat gatggcctcc ggctctgggt
     2881 cgagatgcag cgctcgctgt tggcgcccga agaccaggcc atcgccgaac gtctggagcg
     2941 cctacgggcc gtggaagagc acctcgccgc cgccctcgcc ggtgcgcgcg cggagctcgc
     3001 gtcgcagacg gcgaccgatc gcattcctac gctgcggcgc atggcgagcc tgctcttcag
     3061 ccgccccgat cgcgcagatg cgctcttacc ggtgctgctc gagttggtcg aactcgcgcc
     3121 cgaggagcgc ggctttcagc tcgcggtcga gcgcgtgctc acgcgacaag ggcgcttcga
     3181 agagctcgag gcgttctacc tgcgcgcgct cggccacacc acgctcgggg ccgaacgcgc
     3241 gcggctgtcg tgggcgctct cctcggtccg gcgacggcgc ggagacatcg agggagcgct
     3301 cggaattctg gcgcccatcc tggacgaaga cggcgcgttc ccagcctgca tttcgatgat
     3361 cgtggcgctc tccgcgcaat ccggagagga gctgctgaga gcacgggcgc tgctgcgtct
     3421 cggcgcgtcg ctgccgccgc ctctgcgctc ggtgctatgc gccgtcgcgg cggagatctt
     3481 attgcgcaaa tcggagctca cggccgcccg cagcgcagcg gagcaagcgg tggtggccga
     3541 ctcctcctcg gcgcgcgcgc tggtgacgcg aggcgcggta ggcgccgtgt tgcgcgacca
     3601 ggctggcatc gaagccatgg aacgcgcgct cgcggtggcg gtgccgcgcg cggagctgtg
     3661 cgaagctctg gcctcggcgt acgacgcgat cgagcagcag gaggtctcgt tcgcgtactg
     3721 cttgcgcagg atcgcgctgc ggccctccga tccggtggcc gccgcggacc ggctgactcg
     3781 cgcgacccac agcgatcacg ccgcgcgcct cgccgacacg ctcgcctggc tgctcggcag
     3841 cccgcaaccg ctcgaatcgc tgacgccgct cttggctgct gccttgcggc gcttgtctac
     3901 gctcgagccg ccccgcgcgg cgacgttggc gaagcgcgcg ctcgacgtca tgggtccacg
     3961 ctcgagcgag ctccgcctcg cggtcttggc cgttgcggat ttgaccggcg agcgcgcgct
     4021 cgccatcacg accctcgagc gttggctggc ttcgggcgcg ccgagcgcgg atcgagccga
     4081 gttgctgctc gatgtttcac gccgccggcg tgcgctgacc gacgccgacg gcgcggcgcg
     4141 cgccctgtac cgcgcaatcc gcgagggcgc ctttccgatc tcggtgctcg cggagctcga
     4201 tgtcgcgttg cccgcgaaat ctccggacgg agagttagcc ctgctcgtat ctcgagccga
     4261 ggcgctgagc gcgctcagcg aagcggatgc tcgcagcacc gcgcgcgctt ggcgcgagct
     4321 cggcgcggca taccatgagc tcggacaaga tcgtccggcc gccattgcgg cttgggagcg
     4381 cgcgtttgcg ctcgatccgg atcgcggaat cgagaacttc gcggccgatt tgttggcgtt
     4441 cgatggcccg gagctggcgg tcgccgagct tactcggctg tcggctcatc ggagcgagcg
     4501 cacgcaggcg tgtcgtttgc tcagcgtcgc ggccgcgatt gcgctcgagg ccggcaatca
     4561 ctcgtccgcg ctgtctctgg cggagcgcgc gctgagccta gatccaagcc gaacggacgt
     4621 gctcgcggtg gctgagcgcg ctgcgggtcc cgccgatctc gacatcctgg agaagcttta
     4681 cgatgagctc ggcctggccg cgctcggaca gttcggggcg cgcgccgtcc actaccgagc
     4741 cgcgcggcag ctagagaaac gcggcgaact ggcgcgctcg ttccggcacg ccgtgcgcgc
     4801 gttcgaggcc gtgccctcgg aaggggccgc ctacctggcg atggttcgtt tggcggacgc
     4861 ggtaggcgaa accaccgaag ccgtgcgcgc cctccaggcc gcctcggaag cctgcaacaa
     4921 tccggagctt ggcagtgcat ttctcgagcg cgcggctttg ttgacgggcc ccggcgtaga
     4981 agggcagcgc cagcgggtgg atgtgctgtt gcgctcgctg gtcgtgcgcc ccgagctcca
     5041 caccgtgagt gctctcggcg aagccgtgca ggcgttgatt cgtctggcgc ccgaggagcg
     5101 cgatgttttg gagctgcgcg tccgccgcgc gctcggcagt ttgcttggcc atgttcagga
     5161 cggggagggc gcgcggatcg cgatctcctt cgccgtctgg gtgttgcgcg tgttcaacgc
     5221 ggccggcagc gcagttcagg cgctcgctcg cgccgtcgaa tgccagccca ccctgacgga
     5281 ctacgcggag ctaacgcccc acgcggcggt cctcgcgccc gaagccggcc cgtggttggc
     5341 cagcgctctc gagcgcgcaa agaccgtcag cttcagctcc gagttgcaac gtctgctgct
     5401 cgagatcgcc gatgcccgtg gggatcgcgc gcagattgcc gaactcgccg tgtcactcgc
     5461 agaggccaac ccagacgatg tcgagctggt cgcgcgcgcc gagcgggcgg cgcgcgccgc
     5521 aggcgagcca tcactcatcg cgcgtgcgct cgacgtggtg ccaccagccg agcgccaacg
     5581 ctacctcgaa ctctcggctg acgaggccca gcgtcgaggt gatctccccc gcgccatcga
     5641 gctgatcgaa aaggcgcgca gcctgcccaa catcacccac gaggcgagcc ggggcatgtt
     5701 cgagcacctg atcgagctgc acgaacaact cgcggacgcg gacgcgctcg agcggctgct
     5761 gcaggcggaa atcgagcgcg agacggggga tctcgcgcgg cgcgcacgct tgcgacacaa
     5821 gctcgccctg gttttgtccg aggtgggcga gcgcgagcga tcgctggtcg agatcgagtc
     5881 ggccgccggc gatctgccgt cggagccggc cttgatcgag gactacgttc ggctctcgcg
     5941 ggatgcgttc gacgccatgc gcctaatctc cgcgctgggg ctcggcgtgc gcttgcagcc
     6001 gcagtctact caccagttgc gctggctccg agagctcgcg tcgctgctcg agcaacaagg
     6061 cgatttcgga ctcgcttgcg agcaatgggc gaaagtgctg gagctcgatc cgaaggacga
     6121 tgccgcggcc agcgcgatgg agcgcgacgc ggcccgccgc ggcgactacg aggaggtcgt
     6181 caagtggttg gcccgccgcg ccgagaacac cgcgtcgcat gccgacttgc gcagcattcg
     6241 cttgcgtcgc gcgatggtgc tcgagcagcg cctcgggcgc ccggacgaag cccgggccga
     6301 gctcgaactc ttgacctccg tgatcggcga taacctcagc gtgctgcgcg tgctcgccga
     6361 tctcaacgaa cgtttgggcg cgcccttgaa ggcggcgccc gtctggttac gcggcagtgc
     6421 ggtcacgaag aaccgcgacg aagccgagga tctcgcgtgt cgcgcctgcg aggcttacct
     6481 cgcgagcggc gaagtcgagg ccgctgaacg cgtgctcgag ggcatgcggg cctgggccaa
     6541 ttcacgtcgc atgctgagcc tggcggtaga ggtcgaacgg cgccgcaacc atccgctcgc
     6601 gctcgccgac gcgctcgatg acctcgcgag cttggacgag agccccaccg agcaacgttc
     6661 acgctggctg gtcgaagcgg cccagatctc gttcatggcc gggcaagatc aactggcccg
     6721 agcgcgcgcc gagcgcgcgg cccagctctc accaggcttc gcggaggccg tgctcttggc
     6781 ctgttcgctc gagtaccaac atcgcgagcc cacttcgccg gatcaagcgc tgcgcatcat
     6841 cgaacgtttg gagccgctca gcgtcgcgag cgacgaacaa gccgagctgc gcgcgttctt
     6901 gctcgccgcg gcgctcgaga gcgcgggtcg ccccgtcgcg gcggggctcg ctctcgagca
     6961 ggccgagcgt agtctcggca agcgtccgct gctggcctgg gccttggcgg agaggctcac
     7021 gcggggtggc gagactgagc gcgcgctcgc ctactacgac gcggcgctcg gcggagactt
     7081 gcgcggcctc gggcagcgct cgcgcgtcgc gcttcgggcg gcgcatgccg cgcgcgacat
     7141 cggcgcttcc gagcgtgccg tcggttatct ggagctcgcg gcggaagatc ctgatcaacg
     7201 cgacaccgcg cgcgccgaaa tccaagcctt gctctccgag aatcggggct tcggggcaaa
     7261 gctctccggc ttggacctgc ccgcgaccga agcgcccgtc gtatcgctcg ggcgttactc
     7321 tcaaaagccg cccgcggacg tggcggagga acgcatcgtc gttccaccca ttcgcagcgc
     7381 accgccgcgc tcgggagagc ccgccgccat caaaggcgtg gcgctcgggc gcattttcga
     7441 cgcgcaagag cccacgccga ccaccgatcc tccggaagag gtcatctctt cggacttctc
     7501 ctccactcgc gtcgcgaaat tcgagccgga tcacgccgag ctctacaaag atctctcgtc
     7561 gggttcgttc gaggcggggc gcaagctcat cgccgagctc gagcaacgca gcgaccgcag
     7621 ccatgacctc gtcatggtgt gtcgcaagct ggttttcctg aaccccaccg aactgtgggc
     7681 actccgcagc ctgcgcgagg ccgcgttggc ggacaagaac ctggcttacg cgcgcgccat
     7741 cgagcacgtc ttgggcgtgc tcgagcccga cgatccgtgg gtcgaaccgc ccgcgctcga
     7801 agatcaacct cgcgaaccgg atgccgtgca agccttgctc acgcgggtgg gtcacggctc
     7861 tcccgcgctc gaagcactag cgctcgtttg ggaaggagca gagcgtgtct tccgtcacga
     7921 cccgtcggcg tatggcgtga ccggacgcga acgcattccg cacggagccc ccgcgccgct
     7981 cgggcgcatg tacgggtgca tcgccagtgc tctcggcatg ttgcgagcac ccctcttcca
     8041 acgccgatcc gacggaggcc cagtaaacgt cagcttggcg ctggtctctc cgccagccgt
     8101 gctcgtgacc ggcgaagtcc ggaaagaaac cccggagctc cgcttccacc tcggggccat
     8161 gttgctggcc tgcttgccgc aattcgtgct cctcgttggc tccccggagc cggaagcgcg
     8221 cgccattctg cggggcttga gcttcgcctt cggcccgccc cagctcggct cgatctcggc
     8281 ctcggtgccc aacttggcgg aggtgctttg gcagagcatt ccgcctcgca tgcagcggcg
     8341 tctgcgagag ctaggcagtg acgcgctcgc cttcgaatac gacgcggtga ttcagtccgc
     8401 tcgggtggcg atccgcaggg cggggctttt cgcgtgcggg gacctcggcg tggccctacg
     8461 cgaggccggg ttcgaggacg gaatcgatgg cggaccgggc gaatcgctgc gcaccatcgc
     8521 cgagtcctgg ccgcgcaacc cctcgattcg ttcactactc ttgctcgcga ccagcgccga
     8581 gtacgcgcag gtgcgcttcc agcctgggcg cggcccgcgt tgaccaggcc gtctctctcg
     8641 gagaaagaga cctttgaccc cgcgcgcgca atgggccaca caatgcgcga ggtctgcgct
     8701 ttggaagctc accccagccg tttggaccgt cgtgcccttc gtgtgactag ccggagtgcg
     8761 acacggatgt cgttccaccg cgagcgcgac ggtgaaaact cggcccccgc agaggtggca
     8821 cgcaaatcgc tcttccaaac tccgcggcct tgcccccgct cgaagcactc ccttcctgcg
     8881 cccatttggg cgccgagggc gttcttagcc gcgctgagtt ctgtttgcat gagtgcgtgt
     8941 atcgtggccc cacctgccga atacgaagag tcgacgcgta cgccgcccat attcgacctg
     9001 gttcgagcca agccattcat cggcgacatc atcgtcgtgg accaagacgc gggtttggag
     9061 tccgcgcgca agatccagtt caacgtcccc gtgcgctcgg aagatcaagg cgaagagatc
     9121 atcggtgcgg tgcacatcga ctacggctcg ggccagccgc gggacacctt caccaagagc
     9181 caacagaagc ttcccgcctc gaccctggat gacgtgtcgc gagtctggga tcaaaacgtg
     9241 accttctttg gtggtttgga gcccggttgc caccaagtga ctctgatcgc cacgcacagg
     9301 agcaactggg acgacgagag gttccgcccc atcgtcggcc gtgccgagta cgacaccgcg
     9361 atcgcgatgt ggtggatgat ctacaatccg aatcggcctc cgacgtcccc cgtaacctgc
     9421 ccgtcgggtg cggagatcga gcgttgatcg accaccgtcg cgccagggct ttttcggttc
     9481 ttctcgcgtc gctcgtgggc tccacgggct gcagcttggc ggggctcgag gaacgcgaac
     9541 tgccttattc tcgcaacacc tgcgagaacc aaggggattg ccccgagagc acgacttgtc
     9601 agtccagtgc gggctccagc cgctgcgtct ccacgattgg ctcgtttttg acgttgttgt
     9661 tccgggtttc tccggcggtc tcggcgtcca atctcaccgg gcaggagctc ttccaactcg
     9721 cgacggacgt tcccgccgtc gccaatcgct atgacctcga gctgtctgac ctcaccgaac
     9781 tcagcggcgc cgtgaccgcg agcatcccgg cgggctgcgg cccggcttgg ggtttacccg
     9841 ccagcggaac tggcggtagc tctggaacag gtggcagctc cggatcgacc ggcacggagg
     9901 ggggagctgc tggttcgtcc agtggaggcg cgaacaccaa tcctgggagc gcgggcacgg
     9961 gggctggagg cggcgccacg gagcgttctc ggatcgaagc gcgggtcacg ttcgtcccct
    10021 ctgaaaggcg ctggggtttg gccacggaaa gccacgtcac ccagaccacc cgaaccagcc
    10081 cgtttcgcgc tttgctttca gctggcagct acgacgtcta catcgagccc aagtccgtcc
    10141 ccagcaaccc gggtgaagcc ggggccaacg actgctcgtt gacgccccgt cttttgcgcg
    10201 ggaccaagct ctcgagtggc aataccagcc tgatgctcga tctgccgcag cccacgcggc
    10261 tgcagctgga agtcagagtc atgggcaccg acagcctgga gggcttcagc gtcgacttac
    10321 tcgagcccta ctctggaaga gtggtttcca ctcgacgcca gctttccgcc ccgacgcgga
    10381 cggagggcgg gattcgggtt tacacggtcc aagtcaatta ctcgcccacc gcgaatctcg
    10441 cgtccgacgt ggtcaagagc gagctcgtcc gcttgtctcc gcccgccgga gtcaccgcgc
    10501 ctgcactgta tttcgagcgc acggccctcg agctgttcac caaaggctcg gcaaccatcg
    10561 aattggcaac atcgcttcca aacactctgt ccttcgaggg acaagtcacg gacgcgcagg
    10621 accgaccgca agtatccgca gtctcgctgg tggcgaccag cctagatcgg cttccggcag
    10681 gcacgttggg tggcttcgtg cgcagcatga agacggatga ccaggggcga tttcgcgcag
    10741 agctgttgcc cggaacctac caagtcaccg cgagtccggt ctcatcgcgc gccgatgctc
    10801 cgctcgccac gcgacgggaa acctgggtca tcgcgggaaa accggttcag caagcgggtc
    10861 agaccatcgt cttgcgaccg gccccccgtg tgcgcggtcg gctcctgctg cccgatcggg
    10921 atcagggtgc ggccggggtc acactcacgg cggaagcctc acctctgtcc ttgagtccca
    10981 caatcttaga acgcgagttg ggccaagtat cagccgtccc gagtatcctg agtagcctcg
    11041 tcactcctcc ggatggagtg ttcgaactgt ttgccgaatc cggaacctat gatttctcgg
    11101 cccgcccccc ggacggtgcc gggttcccct ggctcgtatt gccagggctc cgaattccgg
    11161 aaggccacag cgaagtcgtc ctggaagact tgcacctgcc gtggccggtt cccttggaga
    11221 tcagtacatc cctgaaggga cgagaagact ccgcggcttt gctgctcggt gctcatgtcc
    11281 aagcgtatgc actattggac gctcaaggca ggcctaccgc cgatcccgga ttggccgaag
    11341 tcgcggtccc agtcgcggac gcgcgtgtcg acgagcgagg tattgcgacc ttgctccttc
    11401 cagcaagatt gaacgatccg gtgagcactc cgtaagtccg gtgttgacgc tgcgtcatct
    11461 cacacggtgc cgaaacgatc agcacgaggt gcggaaggtt caattaagtt caccttgcta
    11521 cccgaatgac cccgtcgctg acaccgcgga aaaatggctc cgattgattc ctcggggcag
    11581 cagttcgagt atgtgggtgc gctcggatcc agacacatca cagcgttgcg gtgctgacct
    11641 cggacggagg agctgctgct tcgaatccgc agctgcgcgg tgctcctgat cggatgttgc
    11701 acgtttgcgg tcccgaacct cggagtgctg tccaaacctc gatcgtggca agagctcgga
    11761 gcgagtgggc tgaccgatca catttgcacg aaagaactag cgagaagggg acactcataa
    11821 tacagttttc gcgtgacgcg agcacgcctg agttcgatgc tgcccggtga tgattgcgcg
    11881 caagatctcg ccggggccct gcatgaagtc tccaatgcgt tgacggtggt tctcggatgg
    11941 atcgacgtgg cgcacgccaa gttgcctccg ggtcccgaac gtgaggcgct cgaggtagcg
    12001 cgcacgcacg cacgcctcgg ttatcgcatt gcgcggcaat cgatcggcgc ggaagtggcg
    12061 gagcctgcca gcacgcaagg cagtgcgcag tccgtcgcgc gagttgcgat gaccggcgtc
    12121 actccgcaag cgcagcgcgc gggcgtgcgg ctcgtattcg attgcgacaa cccggatgca
    12181 cacactcgcg tctcggacgt aggtgaagca cttcagatcc tcaccaacct gctgctcaat
    12241 gcgatcgcct tcacgccgcg gggtcgcagc gtgacgcttt ccgttcgcga ggacgagaac
    12301 aacgcggtct tccgagtggt cgacgagggg ccgggtcttc ctccggaacg cgcgcgcaac
    12361 gtctttcagg gcccttcctc cacgcgacgg ggaggcgctg gcatcggcct tcgccactcc
    12421 aaatcgctgg ctgagcagca cggcggcagc ctcaatgtcg aagtgttgcc ttcgcccacg
    12481 ttgcccaggc gtccgcccac tccgccttct gctcgccgct cagcgcctga cctccatgcg
    12541 tccggtatcg ttcccgtctc gagcgctacc ccgcccagca tctcgcctcc gaactgtagc
    12601 gagccccgct caggcggggc gtgtttcgaa ctccgatggc cgatcgccga agcgcgatcc
    12661 agcacccggc cccagcaacc cgtcggcgcc agcatcaaag gtgcgcgtgt gctcgtcgtg
    12721 gaagacgacg ccgccgttcg ttccctgatc gaactctcgc tggacgcgcg gggagtggag
    12781 accgtgctcg tgcagagcgc ggccgaactc gagcaagcct tgggagcagc cccgtatgac
    12841 gtcgctctcc tggacatgtc gcccatcgcc gagcacgcgc cggaactcct tcagcgcttg
    12901 cgacagagca gccccggcat tgcgctcatt ctgatcagcg gcctcgccag tcgagttccc
    12961 gaagccatcg agcgggacgt tgccgcctgg gttcgcaagc ccttcgagat gggcgaagtc
    13021 atcgatgtga tgtcgcgcct tcttgccgaa cggcctcccg cgtcctgtcc gcccacgcgt
    13081 cggccctcta cggcaaccgg ctgagcccgc gtcgggccga caccgtcgct tcggagtcgt
    13141 cagtcgagcc aggacctcgg ttcacgagcc ggccccgcga catttcgtcg tccaccctcc
    13201 agatttcgac ttgcgaaccc cagccgccct gaggtagacc ccgtcgccgc agttgccgag
    13261 ggaaaccccg gccgccggac gagacccacg cacctcgtgg ttcacctcat cgcccggctc
    13321 tgcctccggc agccctgcaa acctgattcg gacgcccact caatagagat tcacgacggc
    13381 ataacacgtc caagcaagcc cacgcgaagg tggcggaact ggcagacgca ctagcttgag
    13441 gtgctagcga ggttattctc atgggggttc aagtcccccc cttcgcacaa cacaccggat
    13501 tccggcccgt taccaacccc aactaggagc atcgggctcc tagcgaggtt gccctgaacc
    13561 ggcccgcgca cgggattctc cgtcggcgct ttgaaacccg tagcacccgc cgtggcgaga
    13621 gttcgctcgg cggcgcgacg gttcgcccga atgtacacag cggtcgtgct cacacgctta
    13681 tgccccgcga ggaacgccgc tcccggcaga tttcccgtct ccgcgagctg tgtgagccgc
    13741 gcgtgacgga gatcgtacgc cgcgaacctc ttcgccagat ccgactcgag caccgcgtgc
    13801 gccgccttcc tcaactggtc ccggtactcg tgcacgccga agatcaaacc ttgctcgggg
    13861 cagaccgcat cgagcgctgc tcgcgcctcg gcactcagcg gcaactcgcg cccgaagcgc
    13921 gccttatcga tggcatccgt aatcttcagc gtcgcggctc cgcgtgtgta gtgctcgggc
    13981 acggacagaa cgtcgagcgt cgcgggtctc aacgtcgtct cgaacgctac cacgaaccgg
    14041 gcacgcaccg gaaagcgggg cacgcgtcga gaccccgacc actccggtaa gtggtttact
    14101 aaggcccgcg cctcctccag actgagctcc gtcggcttgc ctcggcgtcg ttgtgcgaag
    14161 gccgtccccg ttgcgcgcct aggtggcttt ggaacaacgg gagccgcatc tagatactcc
    14221 tgctcttcgc accacgcgag gaacccgcgc agagccgaaa gctccttctg caccgtctgc
    14281 ttccgtacga ccccgagccg agctcgagca tagtccgcgg ccttcgccgt cgagaacccg
    14341 gccagcgtct tgaaaaacgg cgcgacgtgc gtcgccccat aggtggtcag atacagatga
    14401 ttcgtctccg gatctcgttc attgcgcgtc tcctgaatcc accgcgccca gagctcctcc
    14461 actcgcgtgt ccggatcgat cgaccacgtc gtcattcccg cttctccttc ttgttttgaa
    14521 tcgcgcgcat ctgatccaag agcgccttct cttcgggtgt cttctgcgac aaggggcgca
    14581 cgctgcgtcc ctgaatcttt cgcgcgaact gggcgtccag ctccgtccag tgaataacgt
    14641 ccggaccgcc ccgcgcgcca taccccggct gcgacggagc cgacgtcgta ttggctggct
    14701 ttgcctgcac gagcaccgcc aaaatcgcat tgctcgcaac tttcaacgtt tcattcaacg
    14761 tcccactcgc cgcgcccagc gcagacgcca gcgtccacgc tgcatctcgc gtactcgccg
    14821 ccagctcatg aattgccccc gccaccccgt ccgccgggat atcccgtgtt ccggaacccg
    14881 cgcactgtga ctctggattt cgtgacttca tctcgattcc ccacctgaaa ggaaagattc
    14941 caagtcacgc cgcctcacca gccgtcgccg tccgggccgc aaactcgcca acttgccttg
    15001 tttgatccag taccgcaccg tctccaccga aacccgcgca atctccgcga cctcatccac
    15061 caaaagcaac tcaccacgct cagtcatgag ccccaagata ggctcaccca acgcctcgtc
    15121 cagcgggaaa tcgcctggga caaatcggtg aaccgggatg tcctggactc cggaacggct
    15181 atccttcgac tactctccgg gctgcgggac atcccggaat ccgcagcgtc aagatatcgg
    15241 ggtgcgggcg cacactcgct ggcgagttgg atctcatgcg aggagccgga gccaggtgcg
    15301 tccttccgcc gtgtgtgggc gaggaggcga tggccttgca gcagtacacg aatctaccat
    15361 tctccgcccc tgtgctctgc aatatgatgc gagcgcttct gatgaagcgc tgaaacgggt
    15421 ggtggtggtg aagtatttcc cgcgggttcc caatatctat atcaacgccg atggctacct
    15481 caagggcttc ctggggctga cttggttccc ttccggggat gccaaagctc atgttgccca
    15541 gtacgagcgc caagtggccg ccgttctctt caacatttgg ggaagaaggg tcggttcag
//
LOCUS       DASTKS010000829         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_355009, whole genome shotgun sequence.
ACCESSION   DASTKS010000829 DASTKS010000000
VERSION     DASTKS010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355009"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1188
                     /gene="nadE"
                     /locus_tag="VFQ35_17110"
     CDS             217..1188
                     /gene="nadE"
                     /locus_tag="VFQ35_17110"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET9932428.1"
                     /translation="MDASVLDLDLPKTVERICERIKESVAGMRKRGAVVAISGGIDSS
                     VCATLSARALGRENVVFLILPERDSSSRSGRLGHMLADHLNVERIVCDIAPTLDAIGC
                     YRWRDDAIRAVFPEYGEGWKNKIVIAGGLERGINFHKLVVEDPSGNRQEKRLGPKEYL
                     QIVAAQNFKQRIRKTLEYFHADRLNYLVIGTPNRLEYDQGFFVKNGDGSADMKPIAHL
                     YKTQVYALARHLGLPAEVVSVRPTTDTFSLEQGQDEFYFALPYEKMDLALWAKNHGRP
                     AAELAAALGISEHQAELVYRDIDAKRRTTRYLHARPELVEPVEEIAK"
     gene            complement(1258..>2325)
                     /locus_tag="VFQ35_17115"
     CDS             complement(1258..>2325)
                     /locus_tag="VFQ35_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET9932429.1"
                     /translation="VEALKTEHASVRCHSSDVAKVFPDVVWHAEAPILRTAPAPLYLL
                     ARLVRESDYRVVLTGEGADEIFAGYDLFREDRVRRFWARHPESTMRPMLLQRLYPYMA
                     RSPVGQLSMAKAFFGKNLTATDDPFYSHRPRWDSTARLKLMLNGDLRLDESAAVERLR
                     ASLPPEISELSPLLRAQFIEVVTLLWGYLLSSQGDRMLMANSIEGRFPFLDPDVMTLA
                     DSLPDGLKLSGLQEKVVLKRAFAERIPQKILRRKKQPYRAPVVKPFISPVVPDYVEEA
                     LSPTAVSAAGLFDVRAVSQLFAKCRATGGERMSNTDEMAFCAVLSLQLLARDLVGGAR
                     LTPPAPAGRLGVDVDRVAGSR"
BASE COUNT          448 a          761 c          757 g          359 t
ORIGIN      
        1 ggtgcgcctg acgcctccgt gagccacggc gcagggtcgc gagccctgtt tggaaaccga
       61 ttgtcgaggt cctgctcgac gagcgagcga ctgctcggct gctcggcccc cggtccaacc
      121 ggaaaccctc acgccgcact aacggggagt gaggaagcga taacgttcgg aaccataccc
      181 gtggtagctt tttccaaact gcggaggcac gcccgaatgg acgccagtgt tctcgatctc
      241 gatctaccca aaaccgtcga gcgtatctgc gagcgcatca aggagtcggt cgccggaatg
      301 cgcaagcgcg gcgccgtcgt ggcgatctct ggtggtatcg acagcagcgt gtgcgcgacg
      361 ctctcggctc gcgcgctcgg gcgcgagaac gtcgttttcc tgatcctgcc ggagcgagat
      421 tcctcgagcc ggagtgggcg cttgggacac atgctcgcgg accacctgaa cgtggagcgc
      481 atcgtttgcg acatcgcgcc cacactcgat gcgatcggct gctatcgctg gcgcgacgac
      541 gccattcgtg ccgtgttccc ggaatacggc gagggatgga agaacaagat cgtgatcgcg
      601 ggtggcctcg agcgcggcat caacttccac aagctggtcg tcgaagatcc gagcgggaac
      661 cgacaagaaa agcgcctcgg gccgaaggag tatctgcaaa tcgtcgccgc gcagaacttc
      721 aaacagcgaa tcagaaagac gctcgagtac ttccacgccg accgcctgaa ttacctggtg
      781 atcggcacac cgaaccgcct cgagtacgac caaggcttct tcgtgaagaa cggggacggc
      841 tcggcggaca tgaagccgat cgcgcacctc tacaagacgc aggtctatgc gctcgcgcga
      901 cacctgggct taccggcaga agtcgtctcg gtgcggccga cgaccgacac cttcagcctc
      961 gagcagggcc aagacgagtt ctacttcgcg ctaccgtacg agaagatgga tctcgcactc
     1021 tgggcgaaga accacgggcg cccggcggca gagctcgcgg ctgctctcgg catcagcgaa
     1081 catcaagccg agctcgtcta tcgagacatc gacgcgaagc gacgcacgac gcgctacctg
     1141 cacgcgcgcc ccgaactcgt cgagcccgtc gaagagatcg caaagtaggg cggttgggcg
     1201 gcgttcgtga ccgcgcgcgc cgcgcaacac tccgcggcct ggggcggagt cgaggcgcta
     1261 ccgcgagccc gcgaccctgt cgacgtcgac gccgagcctt cccgccggcg caggcggggt
     1321 caggcgcgcg ccgccgacca aatcgcgcgc caggagctgg agcgacaaca ccgcgcaaaa
     1381 cgccatctcg tcggtgttgc tcatgcgctc gccgccagtc gcgcggcatt tggcgaagag
     1441 ctgcgacacc gcgcgcacgt cgaacagccc cgcggcgctg accgctgtcg gtgagagcgc
     1501 ttcttcgacg tagtccggca cgacgggcga gatgaacggc ttcaccacgg gcgcgcgata
     1561 cggctgcttc ttgcgccgca aaatcttctg agggatacgc tcggcgaagg cgcgcttgag
     1621 gacgaccttc tcctgcaagc cagagagctt caagccatcg ggcagcgagt cggcgagcgt
     1681 catcacgtcc ggatcgagga acggaaagcg cccctcgatc gagttcgcca tcaacatgcg
     1741 atcgccttgc gacgagagca ggtatcccca gagcagcgtc acgacctcga tgaactgcgc
     1801 gcgcaggagg gggctgagct cgctgatttc cggcgggagc gacgcgcgca gccgctccac
     1861 agccgcgctt tcgtcgagcc gcaagtcgcc gttcagcatg agcttgaggc gcgcggtgct
     1921 gtcccaacgc gggcgatgcg aatagaaggg gtcgtcggtc gcggtgagat tcttgccgaa
     1981 aaacgccttg gccatcgaga gctggccgac cggcgagcgc gccatgtacg ggtacaggcg
     2041 ctggagcaac atcgggcgca tcgtgctctc tgggtgccga gcccagaacc ttcgcacccg
     2101 atcttcccgg aacaggtcgt agccggcgaa gatctcgtcg gccccttcac ccgtgagcac
     2161 cactcgatag tcgctctcac gcacgagccg ggcgagcagg tagagcggcg ccggcgcggt
     2221 gcgcagaatc ggcgcctcgg cgtgccaaac cacgtcggga aagacctttg cgacgtcgct
     2281 cgagtgacaa cgcaccgagg cgtgctccgt cttcagtgcc tcgac
//
LOCUS       DASTKS010000830         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_801029, whole genome shotgun sequence.
ACCESSION   DASTKS010000830 DASTKS010000000
VERSION     DASTKS010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801029"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VFQ35_17120"
     CDS             <1..1938
                     /locus_tag="VFQ35_17120"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9932430.1"
                     /translation="TPAYLAKSSARGAIRERMTELLAIGSISLPVVRRSPRGALRLFY
                     TRRQGSEEQAILYVRDELGGIERVLVDPTLGTDSTTALDWFEPSDDGTLVAYGLSEKG
                     TEDSTLRVRDVASGSDLADVIDRTRYESLAWKPRNSGFFYSRYPEPTTVPAGEERLHR
                     KIYEHVLGTDPQKDRLVFGSELDPTDFPGCATSPKGSWLLVSVSRGFSESALYLAATR
                     DKTEGFARISPAGKALYSGIPREDRLFVLTNDMAPRYRIFAVDPRRPQKEHWRLVVPE
                     HAEDVIRTFDVIGRHILVSYLHRGLTRLARFSLDGKPEGDLSLPALGTSDGFSGLPDG
                     NDAFYAFESFVVPREVRRFDLGKQKDSSFLRVHSDIPSNDYVVESFTARAHDGTEIPY
                     QLVRQARRPLGDGLAKTLLYGYGGFNQIVEPRFSRPVQVFLEQGGVYVQAQLRGGGEF
                     GESWHRAGQLTQKQNTFDDFASVAEDLIERKVTTPAELAILGRSNGGLLVAAALTQHP
                     ELYRAAVAQVPLTDMLRYPRFLIGKLWVSEYGSPDNEPEFRALLAYSPYHRVRPGVSY
                     PSTLITTAESDTRVDPLHARKFAAALQHATSSRRPILLRTERSAGHGAGIPRSKQVDE
                     LTDVYTFLFAELGLQIPPQTR"
     gene            1949..2275
                     /locus_tag="VFQ35_17125"
     CDS             1949..2275
                     /locus_tag="VFQ35_17125"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9932431.1"
                     /translation="MTTAREKMQELHTRILERKSEAAAIGAVYRFILEGEGGGTWVVN
                     LKDDVGVKEGDGAAPCTIRMSGQDAVDLFEGRANGQALFFGQRLKVEGDLALALKLQA
                     LTDILK"
     gene            2310..>2764
                     /locus_tag="VFQ35_17130"
     CDS             2310..>2764
                     /locus_tag="VFQ35_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932432.1"
                     /translation="MPQVTQKSDPPQPNWGGTWPLFQESPGAFPANSQKAVTPFRPER
                     SDLDSLEARADLPETTPQAELERRKLTHSVPPPAPEWVRAARAMIDWLEQRLAQNQVT
                     PTEEAKISRTWAAFSLAGVSEPQALRVAHLVTRAYHAIRETPRSTVELQA"
BASE COUNT          514 a          940 c          845 g          465 t
ORIGIN      
        1 accccggcgt acctcgccaa gtcgtccgcg cgcggcgcga ttcgcgagcg gatgaccgag
       61 ctgctcgcga ttggctcgat ttccctcccc gtggttcggc gctcaccgcg gggggctctg
      121 cgcctctttt acacgcgacg ccaaggctcc gaagaacagg cgattctcta tgtgcgcgac
      181 gaactcggcg gtatcgagcg agtgctcgtc gacccgactc tcggcaccga ctcgacgacg
      241 gcgctcgact ggttcgagcc gtccgacgac ggcacgctcg tcgcctacgg cctcagcgaa
      301 aaaggcaccg aggacagcac cctgcgggtg cgcgacgtcg cgagcggaag cgatctcgcc
      361 gacgtgatcg accgtacccg atacgaatcg ctcgcgtgga agccccgaaa ctcgggcttt
      421 ttttactcgc gctatccgga gcccacgacc gttcccgcgg gcgaggagcg tctacatcga
      481 aaaatctacg agcacgttct cggaacggat ccccaaaagg atcggctcgt gttcgggagc
      541 gaactcgacc ctaccgactt tccgggctgc gcgacctcgc cgaagggcag ctggctcttg
      601 gtcagcgtga gccgcgggtt cagcgagagc gcgctctacc tcgccgcaac gcgcgacaaa
      661 acggagggct ttgctcgcat ttctcccgca ggcaaagcgc tctactctgg cataccgcga
      721 gaagatcgcc tgttcgtcct cacgaacgac atggccccac gctaccgcat cttcgcggtg
      781 gatccgcgcc ggccacagaa agagcattgg cgcctcgtcg ttccggagca cgccgaagac
      841 gtcattcgta cgttcgacgt catcgggagg cacattttgg tctcgtacct acatcgcggg
      901 ctcactcgcc tcgcccgctt ctcgctcgac ggaaagcctg aaggcgacct gagcctgccg
      961 gcgctcggaa cgagcgatgg attctccggt ctgcccgacg gcaacgacgc cttctacgcc
     1021 ttcgagtcgt tcgtcgtgcc gcgcgaagtg cgccgcttcg acctcggaaa gcaaaaggat
     1081 tcgtcgtttt tgcgcgtgca ctccgacata ccgagcaacg actacgtcgt cgagtcgttc
     1141 acggcgcgcg cgcacgacgg gacggagatc ccttatcagc tggttcggca agcgcgacgc
     1201 ccactcggtg acggactcgc aaagactttg ctttacggct acggcgggtt caatcaaatc
     1261 gtggagccgc gcttttcgcg cccggtacag gtcttcctcg agcagggcgg ggtctacgtt
     1321 caagctcagc tgcgcggcgg gggcgaattc ggggaaagtt ggcatcgtgc cgggcagctc
     1381 acccaaaagc agaatacgtt cgacgatttc gcgagcgtcg cggaagacct gatcgagcgt
     1441 aaggtgacga cccccgcgga gctcgcgatc ctgggccgct cgaacggtgg cttgctcgtc
     1501 gccgccgcgc tcacgcagca ccccgagctc tatcgcgccg cggtcgccca ggtgccgctc
     1561 acggacatgc tccgctaccc acggtttctc atcggaaagc tttgggtgtc cgagtacggc
     1621 tcaccggaca acgagccaga attccgcgcg ctgcttgcgt actcaccgta ccaccgcgtc
     1681 cgccctggag tttcctaccc ctctaccctc atcacgacgg ccgaaagcga cacccgcgtc
     1741 gatccgctcc acgcccgcaa gttcgctgcc gcgctgcagc acgcgacgag ctcgcggcgg
     1801 cccatcttgc tgcgcaccga gcgaagcgcg ggtcacggcg ccggaatacc gcggagcaag
     1861 caggtcgacg agctgacgga tgtctacacg ttcttgttcg ccgagctcgg gcttcagatc
     1921 ccgccccaaa ctcgctaagc tgccgggcat gaccaccgcc cgcgaaaaaa tgcaagagct
     1981 tcacacccgc atcctcgagc gcaaaagcga ggcggccgcg attggggccg tgtaccgctt
     2041 catcctcgaa ggtgaaggcg gcggcacttg ggtcgtgaac ttgaaagacg acgtgggcgt
     2101 gaaagagggc gacggcgccg cgccttgcac gatccgcatg agcggccaag acgccgtcga
     2161 tttgttcgaa ggccgcgcca acggacaggc gcttttcttc ggacaacgcc tcaaggtcga
     2221 gggggacctc gcgctcgcac tcaagctcca ggcgctgacg gacatcttga agtgatcgag
     2281 agcgccgagt cgtcgctaaa aatgagggga tgcctcaggt gacacagaag tcggatccgc
     2341 cacaaccgaa ttggggaggc acctggcctc tgtttcaaga gtcaccggga gccttccctg
     2401 cgaattcgca gaaggcagtc acgccatttc ggccggagcg ctccgacctc gattcgctcg
     2461 aggcccgcgc ggatcttcct gaaactacgc cgcaggcaga gctcgagaga cgcaagctca
     2521 ctcactcggt gccgcccccc gcaccggagt gggttcgggc cgcgcgcgcc atgatcgact
     2581 ggctcgagca acggctcgcg caaaaccaag tgaccccgac cgaagaggcg aagatttcac
     2641 gaacgtgggc cgcgttctca ctcgccggcg tctccgaacc gcaagcgctg cgcgtggcgc
     2701 acctcgtgac tcgcgcttat cacgccatcc gcgagacgcc gcgcagcacg gtcgagctcc
     2761 aggc
//
LOCUS       DASTKS010000831         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1919448, whole genome shotgun sequence.
ACCESSION   DASTKS010000831 DASTKS010000000
VERSION     DASTKS010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919448"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFQ35_17135"
     CDS             complement(<1..886)
                     /locus_tag="VFQ35_17135"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9932433.1"
                     /translation="MSTQQKLFIEDIDPSNKRVIVRVDFNVPLKDGVVESDKRLKESL
                     PTIKYLREKGAKVILMSHLGRPDGKRVPEMSLEPVAKALGGLLGAPVKFATDCVGEPA
                     KSAVASLAAGEVLLLENLRFHPEEEENKPEFAKQLAELADVYVNDAFGSAHRAHASTE
                     GITKFTSKAVAGYLMKKELDYLGQAFGNPTRPFVAIVGGSKISSKIDVIKILLPRVDH
                     LILGGGMTFTFMKAQGLEIGRSIVENDKIDFAKSLLKEAGSKLVLPEDFTVTDKLDFK
                     ARTVGQIKTVSKNEIPADW"
     gene            complement(1142..2137)
                     /locus_tag="VFQ35_17140"
     CDS             complement(1142..2137)
                     /locus_tag="VFQ35_17140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9932434.1"
                     /translation="MKILLADDEPIARTMLEHWLSGWGYTVVPVKDGEAALEALKSDP
                     EIRLAVVDWVMPKVDGLGVCRAIRNGPNEPYIYVLLLTARDNKADIVEGLDAGADDYL
                     VKPCNPLELRVRLRAGKRVVELQEQLVGARESLRYEAMHDALTTLLNRRAVLEQLDKE
                     LLRSARRGAPVAVLMGDLDHFKQVNDTYGHAAGDTVLRETARRMKACVRAYDSIGRLG
                     GEEFLTVLPECSAAVAYTVAERIGNVLAQTPIITPAGHIPVATSIGVASTDQHVGATA
                     EQLVRAADVALYRAKHLGRARACLAQPQDFTARRSGEHGAVATLVPPRPSSASTT"
     gene            2373..>2863
                     /locus_tag="VFQ35_17145"
     CDS             2373..>2863
                     /locus_tag="VFQ35_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET9932435.1"
                     /translation="MLRSLRALILFWTVFGSYGLEWVLAYVLPTAIVRARFERAHRRN
                     ARRLARGFSELRGVFIKMGQVLSVMGTFLPAAYGEELEQLQDRVPPRPFSEIRGRLLE
                     AFGDDALGLFASFDEQPIAAASLAQVHRATALDGRRLAVKVLYPGIERLIQRDLAVLR
                     SLLP"
BASE COUNT          442 a          890 c          972 g          559 t
ORIGIN      
        1 cccagtcggc cggaatctcg ttcttcgaca cggtcttgat ctgaccgacc gtgcgcgcct
       61 tgaaatcgag cttgtcggtg acggtgaaat cttcgggcag cacgagcttg ctgccggcct
      121 ctttgaggag actcttcgcg aagtcgatct tgtcgttttc gacgatcgac cttccgattt
      181 cgaggccctg cgccttcatg aaggtgaagg tcatgccgcc gccgaggatg aggtggtcga
      241 cgcggggcag caggatcttg atgacgtcga tcttgctgga gatcttcgag ccgccgacga
      301 tcgcgacgaa cgggcgcgtg gggttgccga aagcctggcc caagtagtcg agctccttct
      361 tcatgaggta gccggcgact gccttggagg tgaacttggt gatgccctcg gtggaggcgt
      421 gcgcgcggtg agcgctgccg aacgcgtcgt tgacgtacac gtcggcgagc tcggcgagct
      481 gcttcgcgaa ctcgggcttg ttctcttcct cttcggggtg gaagcgcagg ttctcgagga
      541 gaagcacttc gcctgcggcg agggaggcga ccgcgctctt cgcgggctca ccaacgcagt
      601 cggtcgcgaa ctttaccggc gcgccgagca agccaccgag cgccttggca acgggctcga
      661 ggctcatctc cgggacgcgc ttgccgtcgg gacgaccgag gtgcgacatc aaaattacct
      721 tcgcgccttt ttcgcgcagg tacttgatgg tgggcagcga ctctttgagg cgcttgtcgc
      781 tttccacgac gccgtccttc aacggcacgt tgaaatcgac gcgcacgatg acgcgtttgt
      841 tggaggggtc gatgtcttcg atgaaaagct tttgttgggt gctcacggca tgctccgtaa
      901 cagagtccga gccgcgcggc tacgctggtt tttacgccgc ggcgcgggcg cggtggcggc
      961 ttcgcgcggc gacgaaaatg gtctgcgcca acgtcgtcac gcgcctccct tgcgcgccct
     1021 ttacgaagct tgcgcctgat cattcattca tctgatcgac atgctcgggc gcggctcgcg
     1081 gaaccgcgcc gtgacgcgcg ccccgcctgg aaagccgtga tttcgtgcgg atcgggccgc
     1141 gctacgtcgt gctcgccgac gacgggcgcg ggggaaccag cgtcgcgacg gcgccgtgct
     1201 cgccgcttcg gcgagcggta aaatcttgcg gctgcgcaag gcatgcgcgc gcccggccga
     1261 gatgtttcgc gcggtacagg gcgacgtcgg cggcgcgtac gagctgttcg gccgtggcgc
     1321 ccacgtgctg gtcagtcgag gccacgccga tgctcgtcgc gacggggatg tggcccgcgg
     1381 gagtgatgat gggcgtctgc gcgaggacgt ttccgatgcg ttcggcgacg gtataagcga
     1441 ccgcggccga gcactccggg aggacggtca agaactcttc accgccgagg cggccgatcg
     1501 agtcgtacgc gcgcacgcaa gctttcatgc gccgcgccgt ctcgcgcagg acggtgtcgc
     1561 cagcggcgtg accgtaagtg tcgttcacct gcttgaagtg atcgaggtcg cccatcagca
     1621 ccgcgacggg ggcgccgcgg cgagcgctcc tgagcagctc tttgtcgagt tgctcgagca
     1681 ctgcgcgtcg gttcagaagt gtcgtgagag catcgtgcat cgcctcgtaa cgaagcgact
     1741 cacgcgcgcc cacgagctgc tcttggagtt cgacgacgcg cttgccggcg cgcaaccgaa
     1801 cccggagctc gagaggattg cagggcttga cgaggtagtc gtcggcgccc gcgtcgagcc
     1861 cttcgacgat gtcggccttg ttgtcgcgcg ccgtgagtag gagcacgtag atgtagggct
     1921 cgttcgggcc gttgcgaatc gcgcggcaca cgccgagccc atcgaccttc ggcatcaccc
     1981 aatcgacgac ggcgaggcgg atctctgggt cgctcttcaa ggcctcgagc gcggcttccc
     2041 cgtccttcac cggaacgacg gtgtagcccc agcccgaaag ccagtgctcg agcatggtgc
     2101 gggcgatcgg ttcgtcgtcg gcgagcagga tcttcatcgg cgactcgcgc gggtcgcacg
     2161 tagacccgcg gggagactcc tctttcaatc caacggttct cggaaccgtg agttaagccg
     2221 caatacagcc gactaccgag ctttccgcaa cgtgcgcccg acgctcaaag cgaaaaataa
     2281 gggattcgac tttcgtcagc cgtgcggccg ggccgttctc gtaggggtgt atcggccctg
     2341 taaccacgat tgggctacgc tgggcggcga ctgtgctgcg ctcgctccgt gcactgattc
     2401 tgttctggac ggtattcggg tcctacgggc tcgaatgggt gcttgcctat gttctgccca
     2461 cggcgattgt acgggcacgt ttcgagcgtg cacaccgccg aaacgcgcgg cgactggctc
     2521 ggggcttcag tgagctgcgc ggcgtcttca tcaagatggg gcaggtgctc agcgtgatgg
     2581 gcacgttcct gcccgcggcg tacggcgaag agctcgagca gctgcaggat cgcgtgccgc
     2641 cgcggccatt ctccgaaatt cggggacgac tgctcgaggc tttcggcgac gacgcgctcg
     2701 gcctgttcgc gtctttcgac gagcagccca tcgctgccgc atctttggct caagtgcacc
     2761 gcgcgaccgc tctcgatggc cggcgcttgg cggtgaaggt gctgtacccc ggcatcgagc
     2821 ggctgattca gcgcgatctc gcagtgctgc gctcgctcct gcc
//
LOCUS       DASTKS010000832         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1248823, whole genome shotgun sequence.
ACCESSION   DASTKS010000832 DASTKS010000000
VERSION     DASTKS010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248823"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VFQ35_17150"
     CDS             <1..1413
                     /locus_tag="VFQ35_17150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9932436.1"
                     /translation="MGLGKTVQLLAHLQRRDEKTGAEARPALLVAPTSVLGNWEREIA
                     RFTPGLRVVAHYSTARARRPEELALGARALVLTSYGLLRRDRELLAAIDWSMVVLDEA
                     QNIKNAASASAAAARSLGASERVALTGTPVENRLAELWSILEFLNPGLLGPLDKFRRE
                     FALPIERYGNEEVAERLRKLVGPFILRRVKSDPAVIRDLPPKNEMKVVCTLTREQATL
                     YKAAVDEEIRRIDSSDGIERKGRVLALLTHTKQICNHPAQYLGESGPLANRSGKLSRL
                     SEMLEEALAVGDKALVFTQFREMGERLRGYLEETLGADVLFLHGGTSKAARDAMVRRF
                     QEEPHGPRVFVLSLKAGGTGLNLTAANHVFHYDRWWNPAVEDQATDRAYRIGQQRSVQ
                     VHKLVSAGTVEEKIDRLLEQKRRLAAKVVGSGEQWITELDGEALRELFALSESSVADE
                     PAPTPEPRRPRRAREKDAQP"
     gene            1410..2360
                     /locus_tag="VFQ35_17155"
     CDS             1410..2360
                     /locus_tag="VFQ35_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET9932437.1"
                     /translation="MSDWETKRFPPRTAKRPPPAHGIKIKKAGTTWWGQRWIAALREV
                     LRGQPGRLERGATYARAGRTHDLRVEGGKVVARVTGSRSTPYEVRIELRPIPGTVWDA
                     AIREMAKKAQFSAELLAGEMPVEIDDVFSPLGASLFPRERSELETECSCPDWGDPCKH
                     VAATHYVLGEALDRDPFLLFELRGRTKDEVLAALRAARGASVVPDRKSIGVDAATVTL
                     QHIAADDYDKPRTPLPALQFSFDEPEEHGALLRQLGAPAAWNGEQTPAETLAPLVRAA
                     AENARRIALAPPREESLREDDPPKAPATARKLKSSRKKRK"
     gene            complement(2332..>2606)
                     /locus_tag="VFQ35_17160"
     CDS             complement(2332..>2606)
                     /locus_tag="VFQ35_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932438.1"
                     /translation="ALGFSEPAWMHLPLVVDANGRRLAKREAALGLAELRERGVDPRA
                     IVAFAARTSGMAVPDLVSVREALGEYRESALPKSDVSITSASFSSS"
BASE COUNT          456 a          857 c          891 g          402 t
ORIGIN      
        1 atggggctcg gtaaaacggt gcagctgctc gcgcacttgc agcggcgcga cgaaaaaacg
       61 ggtgctgaag cgcgtccggc gttgctcgtg gcgccgacat cggtgctcgg caattgggag
      121 cgggagatcg ctcgcttcac gccgggcttg cgcgtcgtcg cgcactactc cacggcgcgt
      181 gctcgtcgcc ccgaggagct cgcgctggga gcgcgggcgc tggtgcttac gagctacggt
      241 ctcttgcggc gcgatcggga gctgctcgcc gcgatcgatt ggtcgatggt cgtgctcgac
      301 gaggcgcaaa atatcaagaa cgcggcttcc gcgagcgccg cggcggcgcg gtcgctcggc
      361 gcgagcgagc gcgtggccct caccggtacg ccagtcgaaa acagactggc ggagctctgg
      421 tcaatcctcg aatttctgaa cccggggctc cttggcccgc tcgacaagtt ccggcgcgag
      481 ttcgcgttgc ccatcgagcg ctacggcaac gaggaggtcg cagagcgcct gcgcaagctc
      541 gtgggcccct tcatcttgcg gcgggtcaag agcgatccgg ccgtgattcg ggaccttccg
      601 ccaaagaacg agatgaaggt cgtctgcacg ctgacgcgtg aacaagcgac gctgtacaag
      661 gccgccgtcg acgaagagat tcgtcgcatc gacagctcag acggcatcga acgcaaaggg
      721 cgcgtgctcg ccttgctcac ccacacgaag caaatctgca atcaccctgc gcagtatctg
      781 ggcgagtccg ggccgctcgc gaatcgctcg ggaaagctct cacgtctctc cgaaatgctg
      841 gaagaggcac tcgcggtcgg cgacaaggcg ctcgtcttca cgcagttccg agaaatggga
      901 gaacgccttc ggggctatct cgaagaaacg ctcggagcgg acgtgctctt tttgcacggc
      961 gggacctcca aggctgcgcg cgacgcgatg gtgcggcgct ttcaggaaga gccccacggc
     1021 ccgcgcgtgt tcgtcctctc gctgaaggcg ggtggcaccg ggctgaacct caccgccgcg
     1081 aaccacgtct ttcactacga taggtggtgg aaccccgccg tcgaagatca ggcgaccgac
     1141 cgcgcgtatc gaatcggcca acaacggtcg gtgcaggtac acaagctcgt ctcggccggc
     1201 accgtcgaag agaagatcga tcggctgctc gagcagaagc gccgcctcgc ggccaaggtc
     1261 gtcggcagcg gagagcagtg gatcaccgag ctcgacggcg aagcgctgcg cgagctcttc
     1321 gcgctttcgg agagcagcgt tgccgacgaa ccagctccca ccccggagcc ccggcgaccc
     1381 cgccgagcgc gggagaagga cgcgcaacca tgagcgattg ggagaccaag cgcttccctc
     1441 cgcgcacggc gaagcgccca ccacccgcgc acgggatcaa gatcaagaaa gccggtacga
     1501 cctggtgggg tcaacgctgg atcgccgcct tgcgcgaggt gttgcggggc cagcccggtc
     1561 gtctcgagcg cggcgcgacg tatgctcggg ccggtcgcac tcacgatctg cgcgtcgaag
     1621 gcgggaaggt cgtggcacgc gtcaccggtt cgcgcagcac gccgtacgag gttcgcatcg
     1681 agctccgtcc gattccaggc acggtgtggg acgcagcgat ccgtgagatg gcgaagaagg
     1741 cgcagttttc ggcggagctt ctcgccgggg aaatgccggt cgagatcgat gacgttttct
     1801 cgccgctcgg cgcaagtctg tttccgcgcg agcgctccga gctcgagacc gagtgtagct
     1861 gccccgactg gggcgatccg tgcaagcacg tcgcggcgac gcactacgtg ctcggtgaag
     1921 cgctggatcg cgatccgttc ctgctcttcg agctgcgcgg ccgcacgaag gacgaggtgc
     1981 tcgccgcgtt gcgtgcggcg cgcggtgctt cggtcgtacc tgaccgcaag tcgattggcg
     2041 tcgatgcggc cacggtaacg ctgcagcaca tcgctgccga cgactacgac aagccgcgta
     2101 cgcctcttcc cgcgctgcag ttttccttcg acgagccaga agagcacggc gccttgctgc
     2161 gccagctcgg tgcgcccgcg gcttggaatg gcgagcaaac acccgccgag acgctcgcgc
     2221 cgctcgtgcg ggccgctgcc gaaaacgcgc gtcgaatcgc gctcgcaccg ccccgcgaag
     2281 agtcgctccg ggaagacgat ccaccgaaag cgccagcgac cgcgcgcaag ctcaagagct
     2341 cgagaaagaa gcggaagtga tggagacgtc gctcttcggg agcgcgctct ctcgatactc
     2401 cccgagtgct tcgcgcacgc tgacgaggtc ggggacggcc atcccgctcg ttcgcgcggc
     2461 gaaggcgacg atggctcgcg gatcgacacc gcgctcacgc agctcggcga gcccgagcgc
     2521 ggcttcgcgt ttcgcgagcc ggcggccgtt cgcgtcgacg acgagcggca ggtgcatcca
     2581 ggcgggctcg ctgaacccga gcgcgc
//
LOCUS       DASTKS010000833         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_801296, whole genome shotgun sequence.
ACCESSION   DASTKS010000833 DASTKS010000000
VERSION     DASTKS010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801296"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..2483)
                     /locus_tag="VFQ35_17165"
     CDS             complement(327..2483)
                     /locus_tag="VFQ35_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HET9932439.1"
                     /translation="MTTRKQAIYEIATSNFVPKTKDSDAPTPDALFGINVFGMAAMKQ
                     HLPKDVFRSLKRTIEKGEPLNPAIADTVAAAMKDWAMSRGATHFAHVFYPLTGATAEK
                     HDAFLSPDGEGGALSEFSGKLLIQGEPDASSFPSGGIRQTSEARGYTAWDVTSPAYIM
                     DSALCIPTAFVSWTGEALDKKTPLLRSMQALNVQAQRVLKLFGHKDCAQVVAYAGPEQ
                     EYFLVDRKFFLARPDLLNAGRSLFGAKPPRGQEFEDLYFGVIPQRVLAFMEEVELELF
                     KLGIPVKTRHNEVAPAQFEIAPVFESANLATDHQHLIMIMLRKIALRHGFECLLHEKP
                     FAGVNGSGKHVNFSFGNSTQGNLLYPGDTPHENAQFLVFCSAVLRAVHKHGGLMRAAV
                     ASASNDHRLGANEAPPAIISAFLGDQLMDVFQQIKAGGAKSSKAGGILEIGVDVLPKL
                     PKDAGDRNRTSPFAFTGNRFEFRAVGSGQSIGGALIVLNTIVAESIDYVAGKLEAAIG
                     SGKTLNAAIQGLLTEMANENLAIAFNGNGYSEEWHKEAEKRGLPNYKTTVESLAVLQA
                     PETVKVFDKYNVLSPRELNSRYEIYLEQYIKTVLVEEKLCTKIAKTQILPAALRYLKE
                     LTDGIGSAKAAGLSPSTALATQVSGLITELEKALGALESAASHSSSSPLDEAKHLRDK
                     VLPAMLQVRTAADALETVVSDDVWPLPTYQEMLYIR"
BASE COUNT          449 a          819 c          927 g          508 t
ORIGIN      
        1 aagagcgcga gcgcgccgaa aaggagcgtg agcgcgccga aaaggagcgt gagcgcgcgg
       61 ctcgagaaga cgtcgaacgg cgcttgcacg agctcgagct cgccatggct cgtagcggca
      121 ggcggtaagc tcaggcagtg ctattcggct ctgcaacgtc gctcaccttc gcggacgatt
      181 aggagcgacg gcgattggcc ggtttgcggt gtctactcgg gtgcggagct cgaggcgttg
      241 cgcgtgcacg aaccgcgaaa tgacgaacgc cgggcaacat cgtttcgatg tgcccggcgc
      301 tcgcgtgccg tcaacgctga atcaaatcag cggatgtaca gcatctcttg gtaggtgggg
      361 agcggccaga cgtcgtcgga gacgaccgtc tcgagcgcgt ccgccgcggt gcgcacctgc
      421 agcatcgccg ggagcacctt gtcgcgcagg tgcttcgcct cgtcgagcgg gctcgacgag
      481 ctgtgcgagg ccgccgactc gagggcgccg agcgccttct cgagctccgt gatgagaccg
      541 ctgacctgcg ttgcgagggc ggtgctcggc gaaagtccgg cagccttggc ggacccgatg
      601 ccgtccgtga gctccttcag gtagcggagc gcggccggga gaatctgggt cttcgcgatc
      661 ttggtgcaga gcttctcctc gacgagcacg gtcttgatgt actgctcgag gtagatctcg
      721 tagcggctgt tgagctcgcg gggcgagagc acgttgtact tgtcgaacac cttgaccgtc
      781 tcgggcgcct gcagcacagc gagcgactcg accgtggtct tgtagttcgg gaggccgcgc
      841 ttctcggctt ccttgtgcca ctcctcgctg tagccgttgc cgttgaacgc gatggcgagg
      901 ttctcgttcg ccatctcggt gagcaggccc tgaatggcgg cgttgagcgt cttgccggag
      961 ccgatggcgg cctcgagctt gccggcgacg taatcgatgg attcggcaac gatcgtgttg
     1021 agcacgatga gcgcgccgcc gatcgactga cccgagccca ccgcccggaa ctcgaagcgg
     1081 ttgccggtga aggcgaacgg gctcgtgcgg ttgcggtcgc ccgcgtcctt cgggagcttc
     1141 ggcagcacgt ccacgccgat ctcgaggatg ccacccgcct tcgaagactt ggcgccgcca
     1201 gccttgatct gctggaacac gtccatcagc tggtcgccga ggaacgccga gatgatcgcg
     1261 ggaggagcct cgttagcgcc caagcgatgg tcgttgctcg ccgaagcgac ggcggcgcgc
     1321 atcagaccgc cgtgcttgtg gacggcgcgg agcaccgccg agcagaacac caagaactgg
     1381 gcgttctcgt ggggcgtgtc acccgggtag agcaggttgc cctgcgtcga gttaccgaac
     1441 gagaagttta cgtgctttcc cgagccgttc acgccggcga agggcttctc gtggaggagg
     1501 cattcgaagc catgacgcag cgcgatcttg cgcagcatga tcatgatgag gtgctggtga
     1561 tccgtcgcga ggttcgcgct ctcgaacacc ggcgcgatct cgaactgagc cggggcgacc
     1621 tcgttgtggc gcgtcttgac cgggatgccg agcttgaaga gctcgagctc gacctcttcc
     1681 atgaacgcga ggacgcgctg cgggatgacg ccgaaataga gatcctcgaa ctcctgaccg
     1741 cgcgggggct tcgcgccgaa cagcgagcga ccggcgttca agagatccgg gcgggcgagg
     1801 aagaacttgc ggtcgacgag gaagtactct tgctcggggc cggcgtaagc gaccacctgc
     1861 gcgcagtcct tgtgcccgaa gagcttgagg acgcgctggg cctgcacgtt cagcgcttgc
     1921 atcgagcgaa gcagcggcgt cttcttgtcg agcgcctcac cggtccacga cacgaaggcc
     1981 gtcgggatgc acagcgccga gtccatgatg tacgcggggc tcgtgacgtc ccacgccgtg
     2041 tagccgcgcg cttcggaggt ttgacggatg ccgccgctcg ggaagctcga agcgtcgggc
     2101 tcgccctgga tgagcagctt cccggagaac tcgctcagcg cgccgccctc gccgtcgggc
     2161 gagaggaagg cgtcgtgctt ctcggccgtg gcgccggtga gggggtagaa gacgtgggcg
     2221 aagtgcgtcg cgccacggct catcgcccaa tccttcatcg cagcggcgac ggtgtccgcg
     2281 atcgcggggt tcagcggctc gcccttctcg atcgtgcgct tcagcgaacg gaacacgtcc
     2341 ttcgggaggt gctgcttcat cgccgccatc ccgaacacgt tgatgccaaa gagcgcatcc
     2401 ggcgtcggcg cgtcagagtc cttggtcttc gggacgaagt tggaggtcgc gatctcgtaa
     2461 atcgcctgct tacgtgttgt catgtgcgtt gttccgtttc cttttgctgg atgcgccggg
     2521 tggttgccga gcggccggta ttccgtcccg tgacgctcat gtactcgccg ctatgcttcg
     2581 ggaatgtttc ggggtgatgc gcggcagtga tctcggtcag actgaaatct gcctcaactt
     2641 attttcaaac cttcgaaact acaccaaaaa ttcgaattga cgcgatgcgt tctgcgaatc
     2701 ccc
//
LOCUS       DASTKS010000834         6884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1920260, whole genome shotgun sequence.
ACCESSION   DASTKS010000834 DASTKS010000000
VERSION     DASTKS010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920260"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1589
                     /locus_tag="VFQ35_17170"
     CDS             60..1589
                     /locus_tag="VFQ35_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9932440.1"
                     /translation="MKFGQTLRVGALACLFSAPLKAQAQAQSAPAAAPKPVKAQAQAP
                     TAAAPKPVKAQAAEAPDAPASPQAPADANGGATPNLPTLDDPMLVTPAPPPHVLTTWQ
                     EALRIVRQNSTALKTARAQIESARAQQRVALAPALPQLTGRAIATDHLIKGEGSVLNA
                     QGGVSTVDLPRPPFSWQAALNLTVPVLASRAWYDYGTARDAVESASLSAKEIERQQVA
                     LVAAAIVSVVTNERLVEVSRLALSSGLSNLDLTKRRAALGAASTVDVLRIEQEVSDSR
                     AQVVAADEALRRAREALGDALGSPEPWGVTPEIRLDALASEAAQSCTRETGIDLRPDV
                     RAAGAAVHVAERNVKSVDYAFVPTIDATSTLTYYSNRYASPNLKQETWTIGAVLNWNI
                     YDGGLRYGTRAARKADRIVAEQRLADTRRRALIEVNQAVRGVQVAEANLTVANRGRQI
                     ADERARLTRIAYMNGTGTSFDLVDAARVARQAELDAAVREFQLLQAKITALLALATCN
                     V"
     gene            1719..2420
                     /locus_tag="VFQ35_17175"
     CDS             1719..2420
                     /locus_tag="VFQ35_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HET9932441.1"
                     /translation="MQRRAFEVLAGVTFVAFAYLIAFIFLKVPGAQLQAGGVAQKILY
                     FHAPAGYAMYLCGGVCLVASAMYLMGATDERDAVAKASAECAVVFGAMMLASGPLWAK
                     KAWGVYWTWDPRLTTTLLSVLIYTSIVVLRAFGGNGDAERRFSAALGILGTVNLPIIH
                     YSVQKWGGNHPTVITKGGGGLKHPDMKLALFMGFAAMTLFAALLLWQRSKLAIGAAKL
                     ARCEEEAAARGLLDS"
     gene            2470..2700
                     /locus_tag="VFQ35_17180"
     CDS             2470..2700
                     /locus_tag="VFQ35_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932442.1"
                     /translation="MQTPTDSRTSPEGRSTEFVAVQGGPETTSAATLMVVAYVAMWGL
                     VFAFLVLGFRRQRRIDQRLNELDRALATRDGR"
     gene            2720..2845
                     /locus_tag="VFQ35_17185"
     CDS             2720..2845
                     /locus_tag="VFQ35_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932443.1"
                     /translation="MTIEHVIFIPGVALIGVIIGYTLGSRAVRAEYERLKRRARE"
     gene            2850..3998
                     /locus_tag="VFQ35_17190"
     CDS             2850..3998
                     /locus_tag="VFQ35_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9932444.1"
                     /translation="MLDFSLSDEQKALVDVARRFARERILPVAAECDREARFPKDVFE
                     AAHALGLVNATVPAEYGGSELGELSNALIAEQLAWACTGIQTSMLANTLALTPILLAG
                     NDEQKRKYLGMLTAGPLFASYCTTEPSGGSDVAGLKTTFKQHGDDFVLNGEKSWITNA
                     SFASFYTVFATSDPQKRHKGIAAFIVDRDTPGLKVGKHEDKLGQRASDTAAVHFEDVR
                     VPKANLLAPEGEGFRLAMETFNQTRPDIGAMATGLMQRCLDESLAYARERKTFGVPIG
                     EHQLVQAMLAEMAISTEGTRLLYQKAAWNLDNGVRDPTISSLAKAFGADRAMQSALDA
                     VQVFGGNGYVRDYPVEKLMRDAKVLQIYEGTSQIQRLVIARNLLKGGG"
     gene            complement(4131..5156)
                     /locus_tag="VFQ35_17195"
     CDS             complement(4131..5156)
                     /locus_tag="VFQ35_17195"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET9932445.1"
                     /translation="MLWFFALSAIAPTVFAYFVVIKSTDRFEPEPVWLLAAAFVWGAV
                     VATLLAIVGNAVGEGVLSAALGVGTGDDLVQRSTASFVAPFVEETTKGTGLLLLWIIS
                     SLALREIDGALDGAIYGGVIGLGFTLTEDVLYVAQAGEQGGLGAFFSVFVLRTLFSGL
                     GHASFTAMTGLGVGIAAEAASGPLKLLAPIGGWMAAVGLHMVHNFLVTFLFEGGAGLG
                     LKLVTFWCFDVLFFVVIVVLAVRDRAIVLRGLIDEAGRLLHPRELAQTTSYWMLVPLW
                     NFFSLMGSQSGYSRSRKKQLDLIELAFLKQRRRRGESDLGLERAERTLRERIWHANQA
                     GVFIGKR"
     gene            5225..5794
                     /locus_tag="VFQ35_17200"
     CDS             5225..5794
                     /locus_tag="VFQ35_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932446.1"
                     /translation="MFGFSFGEFLLICVVALVAVGPQKLPGLLRTLATWIRKARHMIH
                     DMRTQSGIDEILRAEGLHGGLNELRHLVRTNIPTDLVASAMTPPPPQPVPGIVTPSSE
                     VTLDPAVAPAIAPAVTSPSLYEDLYGGVDIDPTKEYPPEGADSYGALPDDLISDMPAY
                     PLPPSPVAASPEAAETETNPEARSASPAP"
     gene            5791..6597
                     /gene="tatC"
                     /locus_tag="VFQ35_17205"
     CDS             5791..6597
                     /gene="tatC"
                     /locus_tag="VFQ35_17205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9932447.1"
                     /translation="MSNASPMSAEPTNEQDPSGSNSEEGTMTIWEHLEELRSRIIRMI
                     IAALAGGAGAWIYREDVLKWLVKPFQEAGGIDLNFQSPAAMFISYVRIAAMAGFIFAL
                     PIILYQVWAFIAPGLYSREKKFAIPFVLSSCGLFALGGWFGWRFAFPLAFKYLLSFAG
                     TVGGIQIKATVMVSEYLDFVSRMLLAFGFAAELPVLVFFLAIAGLVDHKSLIKFFRYF
                     VVIAFVISAILTPPDPLSQLMLALPLCLLYGISILVAYIFSKRKKDSADS"
     gene            complement(6671..>6884)
                     /locus_tag="VFQ35_17210"
     CDS             complement(6671..>6884)
                     /locus_tag="VFQ35_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET9932448.1"
                     /translation="LERWLAQGKGVIAFAIGGAEGLPAEFSKRAQHRLSLSRLTLPHR
                     LARLLLAEQLYRALSIARGEPYARED"
BASE COUNT         1241 a         2318 c         2197 g         1128 t
ORIGIN      
        1 gaatcgccac atcgagtgtc gttgatcgac gcggcacgag ccgtgctatt cagcacgcca
       61 tgaagtttgg ccagacactt cgtgtgggtg ctctcgcgtg cctgttttcg gctccgctca
      121 aggctcaggc gcaagcccag agcgcgcccg cggcggcacc gaaaccggtc aaagcacaag
      181 cacaagcgcc gaccgcggcg gcaccgaaac cggtcaaagc acaagcagca gaggctcctg
      241 atgcgccggc atcgcctcaa gctcccgccg acgccaacgg cggcgcgacg ccgaatctcc
      301 ccacgctcga cgatccgatg ctcgtcacgc cggcgccgcc gccgcacgtg ctcacgacgt
      361 ggcaagaggc gctgcgcatc gtgcggcaga actccactgc gctgaagacc gcgcgcgcac
      421 aaatcgagtc ggctcgggcg caacagcgag tcgcgctcgc accggcgctt ccgcagctca
      481 cgggtcgcgc catcgcgacg gatcatctca tcaaaggcga aggcagcgtt ttgaacgcgc
      541 aaggcggcgt ctcgacggtc gaccttccgc gaccaccttt ttcgtggcag gccgcgctga
      601 atctcacggt gccggtcctc gcttcgaggg cttggtacga ctacgggacc gcgcgtgacg
      661 ccgtcgagag cgcgtcactc agcgcgaaag aaatcgagcg gcagcaggtc gcgctcgtcg
      721 cggcggcaat cgtctccgtc gtgacgaacg agcgcttggt cgaagtgagc cgcctcgccc
      781 tgtcgagcgg cctctcgaac ctggatctca ccaagcgacg cgcggcgctc ggcgcggcga
      841 gcacggtcga tgtgttgcgc atcgagcaag aggtctcgga ttcgcgagcg caggtcgtcg
      901 ccgccgacga agcgctgcgt cgagcgcgcg aagcgctagg cgacgcgctc ggctcgccgg
      961 agccttgggg agtgacgccg gagattcgcc tggacgcttt ggcgagcgag gcggcgcagt
     1021 cttgcacgcg cgagactggc atcgaccttc gcccggacgt gcgcgcagcg ggcgcagccg
     1081 tgcacgtcgc cgagcgcaac gtcaagtcgg tggactacgc cttcgttccc acgatcgacg
     1141 cgacctcgac gctcacctac tactcgaacc ggtacgcctc gccgaacctg aagcaggaga
     1201 cctggacgat tggcgccgta ttgaactgga acatctacga cggcggcctg cgctacggca
     1261 cccgcgccgc tcgcaaggca gatcgcatcg tcgccgagca gcggctcgcc gatacgcgcc
     1321 gccgcgcgct catcgaggta aaccaggccg tccgcggcgt tcaggtcgcc gaggcaaacc
     1381 tcaccgtcgc gaaccgcggg cggcaaatcg cggacgagcg cgcgcgcctc acccgcattg
     1441 cctacatgaa cggcaccggc acgagcttcg acctcgtcga cgcggctcgg gtcgctcgcc
     1501 aagccgagct cgacgccgcg gtgcgagaat tccagctttt acaggccaag atcacggcgc
     1561 tcctcgcgct cgccacctgc aacgtctgag tcgggcgatg gcgcgggccc agggccgagc
     1621 cccacggctc gcggcgccga gcccacgcct cgcaaaagtc agcgctcgag cgcgcgccgt
     1681 ttgcgctcga aacgcgagga agtgctagtc tgccgcccgt gcaacgccgc gcgttcgaag
     1741 tgctcgcggg ggtcaccttc gtggctttcg cttacctcat cgccttcatc ttcttgaagg
     1801 ttcctggtgc gcagctgcaa gccggcggcg tcgcgcagaa gatcctctac tttcacgcgc
     1861 ccgctggcta cgcgatgtac ctctgcggcg gcgtgtgtct cgtcgcgagc gcgatgtacc
     1921 tgatgggcgc gaccgacgag agagacgccg ttgccaaagc gagcgccgag tgcgcggtcg
     1981 tgttcggcgc gatgatgctg gcgagcggcc cgctctgggc gaagaaggct tggggcgtct
     2041 actggacatg ggaccctcgc ctgacgacca cgctgctcag cgtgctcatc tacacgtcga
     2101 tcgtcgtgct gcgagctttc ggaggcaacg gggacgcgga gcgccggttc tccgccgcgc
     2161 tcggcatcct cggcaccgtg aaccttccga tcatccacta ttcggtgcaa aagtggggcg
     2221 gcaatcaccc gaccgtcatc accaaaggcg gcggcggcct gaagcacccg gacatgaagc
     2281 ttgcgttgtt catgggcttc gccgcgatga cgcttttcgc cgccctcttg ctctggcaac
     2341 gctcgaagct cgcgatcggc gcggcaaagc ttgcgcgctg cgaggaagag gcggccgcgc
     2401 gggggctgct cgactcgtag agcgcccgcg accgattggc cccaatcgtg attttcttgg
     2461 agatcgaacg tgcaaacacc gactgattca cgaaccagcc ccgaaggccg ctccaccgag
     2521 ttcgtggccg tacagggagg ccccgagacg accagcgccg ccacgctgat ggtcgtggcg
     2581 tacgtggcca tgtgggggct ggtgttcgcg ttcttggtac tgggcttccg gcgccagcga
     2641 cgcatcgatc agcgattgaa cgagctcgac cgcgcgctcg cgactcgcga cgggcgctga
     2701 ggcactgagg agctcggcca tgacgatcga gcacgtcatt ttcattcccg gggtagcgct
     2761 catcggcgtg ataatcgggt acacgctggg ttcgcgcgcg gtccgcgccg agtacgagcg
     2821 tctcaagcga cgagcaaggg agtgagcgga tgttggattt ttcgctgagt gacgagcaga
     2881 aggcactggt cgacgtggcg cggcgcttcg cgcgggagcg catcctcccc gtggccgccg
     2941 agtgcgatcg cgaggcgcgc tttcccaagg acgtcttcga ggccgcgcac gcgctcggac
     3001 tagtgaatgc gaccgttcct gcagagtacg gcgggtcgga gctcggcgaa ctttcgaatg
     3061 ccctcatcgc cgaacagctc gcgtgggcgt gcaccggtat tcagacgagc atgctcgcca
     3121 acaccctcgc gctcacgccg attttgctcg cgggcaacga cgagcagaag aggaagtacc
     3181 tcggcatgct caccgcaggg ccgctgttcg cgagctactg cacgaccgag ccgagcggcg
     3241 gcagcgacgt cgcaggcctg aagacgacgt tcaaacagca cggcgacgac ttcgtcctga
     3301 acggcgaaaa gtcgtggatc acgaacgcga gcttcgcgag cttctacacc gtattcgcga
     3361 cgagcgaccc ccagaagcgc cacaaaggga tcgccgcctt catcgtcgat cgcgacacgc
     3421 ccgggctgaa ggtcggcaag cacgaagaca agctcggcca gcgcgcgagc gacaccgccg
     3481 ccgttcactt cgaagacgtg cgcgtgccga aagcgaacct gctcgcgccg gagggcgaag
     3541 gcttccgcct cgcgatggag accttcaacc aaacgcgccc cgacatcggc gcgatggcga
     3601 ccggcctcat gcagcgttgc ctcgacgaga gcctcgctta cgcgcgcgaa cgtaagacct
     3661 tcggcgttcc catcggcgag caccagctcg ttcaggccat gttggcggag atggcgatca
     3721 gcaccgaagg gactcgcctg ctctatcaaa aagccgcgtg gaacctggac aacggcgttc
     3781 gtgaccccac catctcgagc ctggcaaagg ccttcggcgc cgaccgcgcg atgcaaagcg
     3841 cgctcgacgc agtgcaggtg ttcggcggca acggctacgt ccgcgactac ccggtcgaga
     3901 agttgatgcg cgacgccaag gtcctccaga tttacgaggg cacgagccaa atccagcggc
     3961 tcgtcatcgc gcgcaatttg ctgaagggcg gcggctgagc gcctgaaccc ttttcggcgc
     4021 gagcgaagcg ccgagcgcca aacccttctc ggcacgagcg aaacgccgag cacccgagcc
     4081 gttttcggcg cgagcgagca gacggacggc tcgatccgcg agaccgtccg tcagcgtttg
     4141 ccgatgaaga cgccggcttg attggcgtgc cagatacgct ctcgcaaggt gcgctcggcg
     4201 cgctcgaggc cgagatcgct ctcgcctcgt cggcgccgct gcttcaagaa cgcgagctcg
     4261 atgaggtcga gctgcttctt tcggctgcgc gagtagccgc tctggctgcc catcagcgaa
     4321 aagaagttcc agagcggcac gagcatccag tagctcgtgg tttgcgcgag ctcgcgcggg
     4381 tgaaggagcc gccccgcttc gtcgatgagc cctcgcagca cgatcgcgcg atcacgaacg
     4441 gcgagcacca cgatcacgac gaagaacaac acgtcgaagc accagaacgt cacgagcttg
     4501 agtccgagcc ccgccccgcc ctcgaacaag aacgtgacga ggaaattgtg caccatgtgc
     4561 aagcccaccg cggccatcca gcccccgatg ggcgcgagca atttgagtgg ccccgacgcc
     4621 gcttccgctg cgatgccgac cccgagtccc gtcatcgcgg tgaacgacgc gtgaccgagc
     4681 cccgaaaaca gcgtgcgtaa gacgaacacg ctgaagaacg cgccgagtcc cccttgctcg
     4741 ccggcctgcg ccacgtagag cacgtcttcg gtgagggtaa agccaagtcc gatgacgccg
     4801 ccatagatcg cgccgtcgag cgcgccgtcg atctcacgca acgctaggct cgaaatgatc
     4861 cagagaagga ggagtccagt ccctttggtc gtttcttcga cgaagggcgc gacgaacgaa
     4921 gccgtcgagc gctgcacgag gtcgtcccca gttccgacac ccagggcggc gctcagcacg
     4981 ccttctccca cggcgttgcc tacgatggcg agcaacgtgg ccaccaccgc accccacacg
     5041 aacgccgccg caagcaacca gaccggctcg ggctcgaagc gatcggtcga cttgatcacg
     5101 acgaaatacg caaaaacggt gggcgcgatg gccgagagcg cgaaaaacca gagcacggcc
     5161 gcattttacc tctagccaac gagccgaggt tgcgagtttg gcacggcaaa gctaggttcc
     5221 gttcgtgttc ggcttctcat tcggcgagtt cttgctgatc tgcgtggtcg cgctcgtcgc
     5281 cgttggtccc cagaagttgc cgggcctcct ccgcaccctg gcgacgtgga tccgcaaagc
     5341 gcgccacatg atccacgaca tgcgcacgca gagcggcatc gacgagatct tgcgcgccga
     5401 gggccttcac ggcggcctga acgagctacg ccacctggtc cgaaccaaca tcccgaccga
     5461 cctcgtggcg agcgccatga ccccgcctcc gccgcaaccc gtgccgggca tcgtgacgcc
     5521 ctcctcggag gtgacgctcg acccggccgt cgcgcctgcg attgcgcctg ccgtgacgtc
     5581 acctagcctc tacgaagatc tttacggtgg cgtcgatatc gatccgacca aggaatatcc
     5641 gccggaaggg gcggattcat acggtgcgtt gcccgacgac ttgatctcgg acatgccggc
     5701 gtatccgttg cctcccagcc cggtcgcggc gagcccggaa gcggccgaaa ccgaaacgaa
     5761 ccccgaggcg cgctcggctt cccctgcccc atgagcaacg caagcccgat gagtgccgag
     5821 cccacgaacg agcaagatcc ctccggctcg aacagcgaag aaggcacgat gacgatctgg
     5881 gagcacctcg aggagctccg gtcgcgcatc attcgcatga tcatagccgc cctggccggc
     5941 ggcgcgggcg cgtggatcta ccgcgaagac gtcctgaaat ggctggtcaa gccgttccag
     6001 gaggcgggcg gaatcgatct gaacttccag tcgcccgcgg cgatgttcat ttcgtacgtg
     6061 cgcatcgcgg ccatggccgg gttcatcttc gcactcccaa tcatccttta tcaggtgtgg
     6121 gcgttcatcg ccccgggcct ctattcgcga gaaaagaagt tcgcgattcc gttcgtgctc
     6181 tcgtcctgcg ggctgttcgc gctgggcggt tggttcgggt ggcgcttcgc cttcccgctc
     6241 gcgttcaaat acctgttgag cttcgccggc acggtcggtg ggattcaaat caaggcgacc
     6301 gtgatggtga gtgagtatct ggacttcgtg tcccgcatgc tcctcgcctt cgggtttgcg
     6361 gccgagctcc cggtcctcgt attcttcctc gcgatcgcgg ggctcgtcga tcacaagagc
     6421 ctgatcaagt tcttccgcta cttcgtggtg atcgcgttcg tgatctccgc gatcctcacg
     6481 ccgcctgatc cgctgagcca gctcatgctg gcactcccgc tctgtcttct ctacggcatt
     6541 tcgattctgg tcgcgtacat attctcgaag cggaagaagg acagcgcgga cagttgacgc
     6601 ttgagccgtc acgaacgtag agctccgcgc gcgattcagt ctccctgagt gcagagatcg
     6661 gcgcgcgact tcagtcttcg cgcgcgtagg gttcgccgcg cgcgatgctg agtgcgcggt
     6721 agagctgctc ggcgaggagg agccgcgcga gccgatgagg tagcgttagg cgcgacaggc
     6781 tcaaccggtg ctgggcgcgc ttggagaact ccgctggcag cccctccgcg cctccgatgg
     6841 cgaaagcgat cacgcccttt ccctgcgcga gccagcgctc gagc
//
LOCUS       DASTKS010000835         8026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1920296, whole genome shotgun sequence.
ACCESSION   DASTKS010000835 DASTKS010000000
VERSION     DASTKS010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920296"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFQ35_17215"
     CDS             <1..917
                     /locus_tag="VFQ35_17215"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9932449.1"
                     /translation="LGAKSMNLLGTSYGTRLAQEIMRQAPESLRSVTLDSTLPPDLDA
                     LAQTAVAAERAFRQVFIACAEDLTCREAFPDIEARFYEVVATLNEHPLLVQNGDEQTR
                     IDGERFVQLLGLLQYDINGVVRIPGIIAEAESGQSDTIQQLDLLRAAPDAGIAAGMNL
                     SVVCAEYAPLTSVDKVALAERGVRPEISSVFNAQKYLVACPIWSVPAAPATESMPVRS
                     SVSTLILEGSFDPSTPPSWGDHVASGLSRAHIALIGAATHGVARTECGMTALASFVHD
                     PANYTHPGCVAQSSPQLWTQAAMSGAMK"
     gene            complement(974..1636)
                     /locus_tag="VFQ35_17220"
     CDS             complement(974..1636)
                     /locus_tag="VFQ35_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932450.1"
                     /translation="MQTHLRHRAFKYVAALAIAASCGALRARPKLLEIPETLVYELVE
                     AVCEDEPNWSKEDRQRVLTLLSRDPRVAVRARVAEAAVALSGEWQTDTQRLLSSLATD
                     SAGTVRAAATNGLERLLTRASPIERVEIVCQWAAAPHAAEREAIARALCSKVPTLVTD
                     LVLEQLSRDDAAEVRAVAVRASRVHFDENPGAYRSLLEERTADPSRRVRRVARRMLAD
                     VA"
     gene            1858..3534
                     /locus_tag="VFQ35_17225"
     CDS             1858..3534
                     /locus_tag="VFQ35_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9932451.1"
                     /translation="MPSSFSPPASSRSGPRIALLLSYLSNEYEWSIVRGARAVVEAAG
                     GSVVCVVGGAIGDPRRERSVRNTLFDLVDAANVDGIVAVSSVLGQFIGDEAMREWLGR
                     YQLPVCSIGRVEGVPSVEIDDAIGVVSLVSHLIEYHEHRHIAFIGGPEGNREAQARLG
                     AYRKALEEHGIAFDPRLVTTGEFTWPSGARALRQLLDERQVPLSSLDSIVAASDYTAF
                     GVIEELARRRIEVPEHVAVVGFDDMPRASVHQPPLTTVKQQIDELGREAARIVLGLLE
                     GRPAEPVVHLTTDLVLRRSCGCTPTDVPSGDEDASSDELPPADQRIQPRGGAWSLELV
                     AALAAEASGQMNAFEKALDPLLQRMIVAGVDLSLVQDMILFLGQRAIRQLGGDSRFTQ
                     RIESAVQRGRVLVSELTSRADRMPEDSISEQLHIFSRALLSRMFGPQTQLSAVLVEHL
                     PHFGIDECLVSEFVQPSSVAELRPAFGFDNRHVQPQLQAYPGRELAPPDFEGMHHHSV
                     FLLPVRYGEQAIGIVLLPPCGHDGTVYETLAEVFGIALKGIEMRRKAEAR"
     gene            3590..4228
                     /locus_tag="VFQ35_17230"
     CDS             3590..4228
                     /locus_tag="VFQ35_17230"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET9932452.1"
                     /translation="MRVDVWTDFICPWCYLGKRRLELALEQFAGRSTVEIHWRSFQLD
                     PEFPREFSGGVNDLLVNKYGMSRAEAEAIHARVTGLGAADGLVYRFDRCRPSNTFDAH
                     RLLQAANRRGVGAALQDRFMRAYFAEGISLGDAPSLRALASEAGLEAPDSDAVLSGDA
                     FGEQVSADQRRAQELGYRGVPAFLFAEKVQVSGAQSPETLLAALQRAERASS"
     gene            complement(4201..4890)
                     /locus_tag="VFQ35_17235"
     CDS             complement(4201..4890)
                     /locus_tag="VFQ35_17235"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9932453.1"
                     /translation="MKLVCFDLGGVLVRICRSFGEACRAAGLDVRASIEGRVAQRIRY
                     EFSQRFNTGKLDELSWANELSGALDGLYTADELRRVHRAWTLGEYEGAHELVHELDSI
                     GVATACLSNTDQSHWTRLVHHDGTSALPGEPEYPSVLRLHHRHASHLFGFAKPNAEIY
                     RAFEAATALRGPEILFFDDLPENVRGARDIGWRAERIDPYNETVPQLRRWLRHHGVLP
                     RTAQELARSAR"
     gene            complement(4959..6365)
                     /locus_tag="VFQ35_17240"
     CDS             complement(4959..6365)
                     /locus_tag="VFQ35_17240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9932454.1"
                     /translation="MMLRSRLVYRLYAIGVVQLFLVAVAAVGIAYAMAKTPPRWDMQS
                     ITAELTRVVLDRHALGGELEMLRRRHGLLLSLYDEDRHLLASNVEPPLHPPRLSPRLD
                     PAPLAAPPRGNGPPPSPPLVPAFGHGPNGHVPPHMYAPISIEGREGVLVARFERPRPS
                     LWMPSLTIAAGLVVVGAGALLTARWIAGPLQQLSRASAAIGSGELNARTGLARSDEIG
                     DVARAFDNMADRIQALRAAEKELLANVAHELRTPLSRIRVALEIASEGDAEIVKSSLS
                     EIAVDLAELEALIDDVLNATRFELSDVKATPTHLALHVEQLSPLRVAKDSAERFAARH
                     PTRPFTTRFDDSLPPIEADPVLLRRVLDNLLENAQKYSPDESAPIELCVSKEQSSVVF
                     QVKDNGMGIEEGDLPHVFEAFFRAERSRSRGTGGVGLGLTLAKRIVEAHSGSISVASV
                     KGRGTSVTARIPWQVLSA"
     gene            complement(6362..7084)
                     /locus_tag="VFQ35_17245"
     CDS             complement(6362..7084)
                     /locus_tag="VFQ35_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9932455.1"
                     /translation="MVSPEAASSYELLTVVYVEDDARLARLTAQYLRSHGVDVTIVPS
                     GEVAFAEITRVRPDVVLLDLMLPGADGLEVCRRVRERMDVPIIMVTARTDEADRVLGL
                     EGGADDYVSKPFSSRELLARIRAHARRARGRAGPTPERIEVGPLVVDAGTMSATLNGS
                     RLSLTTYEFSLLRVLAERAGRVLGREQLLSLLHGTAEEAFDRSIDVHVSRLRHKLEDD
                     PRNPRLLKTIRGVGYMLAPEPP"
     gene            complement(7231..>8026)
                     /locus_tag="VFQ35_17250"
     CDS             complement(7231..>8026)
                     /locus_tag="VFQ35_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932456.1"
                     /translation="AFSQFSRCSGLSRALSVADAAPVEQRWQTLRNELRKELPLCRCS
                     DLIAPSLKPIVVAEQRAGTATLAALPFGFIRDERCGASMPLRSVGKLLEQMERFDEEF
                     SGGWQRDALAFEKVLTEERLLNAFCNALPGETLAQLTKSKATLYWKVAGSDVCEAFRF
                     EPLSPGAPMGTLREVAPQAHGARAFHFWQGAEEIRVFGPIPPGVASKPTDEREWTCDQ
                     NLKLTAVNADSLEFEHGRWFFSEAGCKAAAPNTAVEGCIGKRAETP"
BASE COUNT         1352 a         2563 c         2714 g         1397 t
ORIGIN      
        1 cgcttggcgc caagagcatg aatttgctcg gcacatctta cggcacgcgc ctggcgcaag
       61 aaatcatgcg acaagcgcca gagagtctgc gtagcgtgac gctagactcg acgctgcccc
      121 ctgacttgga cgcgctcgct caaactgcgg tcgccgctga gcgcgcgttt cgacaagtgt
      181 tcatcgcttg tgccgaagac ctgacttgtc gcgaggcgtt tcccgacatc gaggcgcgct
      241 tctacgaagt ggtggcgact ctgaacgagc atcctctgct ggtgcagaac ggagacgagc
      301 aaacccggat cgatggcgag cgcttcgtgc agctactcgg cctgttacag tacgacatca
      361 atggcgtcgt gcggatcccc ggcatcatcg cagaagccga gagcgggcaa tccgacacca
      421 tccagcagct ggacttgctg cgcgcggccc ccgacgctgg tattgcagct ggcatgaacc
      481 tgtcggtggt gtgcgccgag tacgctccgc tgacgtccgt tgacaaggtt gcgctagccg
      541 aacggggggt gaggcccgaa atctcttccg tgttcaacgc acagaagtac ctggttgcgt
      601 gcccgatttg gtcggttcct gccgcaccgg cgaccgagtc gatgccggtg cgttcgtccg
      661 tgagcacgct gatcctcgag ggcagttttg atccgtcgac cccaccgagc tggggcgacc
      721 acgtggcgag cggcctctcg cgcgctcaca tcgcgttgat cggggccgcc acacacggcg
      781 tggcgcgtac cgagtgcggc atgacggcgc tcgcgagctt cgtccacgac ccagcgaact
      841 acacgcaccc gggctgtgtc gcgcaaagct ccccgcagct ctggacgcag gccgccatgt
      901 ccggggcgat gaagtgattg cgccgatctg gtgttcgacg cgcctaggtg tgcgcttcgg
      961 aaaacgagcg atgtcaggct acatcggcca acattcgtcg cgccacgcga cgtactcggc
     1021 ggctcgggtc cgcagtgcgc tcctcgagaa ggctccgata ggcgccaggg ttttcgtcga
     1081 agtgcactcg gctcgcgcga accgcgaccg cgcgcacctc ggccgcgtcg tcgcgcgaca
     1141 gctgttcgag cacgagatcc gtgaccaacg tcggcacctt cgagcaaagc gcccgggcga
     1201 tggcttcgcg ctcggcggcg tgtggcgccg ccgcccactg acagacgatt tcgacccgtt
     1261 cgatgggact cgcccgcgtg agcagtcgtt ccagcccgtt ggtcgccgcc gcgcgcacgg
     1321 ttccagcgga gtcggtggcc aagctagaga ggagccgctg tgtgtcagtt tgccactcgc
     1381 ccgacagcgc gacagcggcc tcggctacgc gcgctcggac ggctacgcgg gggtcgcgcg
     1441 acaaaagcgt caatacgcgc tggcggtctt ctttcgacca attcggctcg tcctcacaga
     1501 ctgcctcgac gagctcgtac acgagcgtct cgggaatctc caaaagcttg gggcgtgcgc
     1561 gtaaagctcc gcagcttgcc gcgattgcga gggccgcaac gtacttgaag gcccgatgcc
     1621 gcaggtgagt ctgcatgtga gctgaaacct ccgtgaaggg ggaattcgtc gcgcgtgtgc
     1681 agggaaccat cggggtgcgc gggctattcc cggggcgacc gttgaagcgg tgctgcagac
     1741 tttttgcctt tcacgtttca tcggcttgcc aggcggcgac gtcgcggacg gtggtcgtga
     1801 gggggcgttg gccaacccgg tccagccgac tggaaattgc gctagcgtcg taagaccatg
     1861 ccctcgtcct tttcaccgcc cgcgagctca cggagcggac cacgtattgc cctgctgctc
     1921 tcgtacctga gcaacgagta cgagtggtcg atcgtgcggg gagcgagggc ggtggtcgag
     1981 gccgctggcg gcagtgtcgt ttgcgtcgtg gggggcgcaa tcggtgaccc gcgccgggag
     2041 cgctcggtga gaaacacgtt gttcgacttg gtcgacgcgg ccaacgtgga cggcatcgtg
     2101 gccgtctcga gcgtcctcgg acagttcatc ggcgacgagg cgatgcgaga atggctcgga
     2161 cgctatcagc tgccggtctg cagcatcgga cgcgtagaag gtgtccccag cgtcgaaatc
     2221 gacgacgcga tcggtgtcgt gtcgctggtg agccatctca tcgagtacca cgagcaccga
     2281 cacatcgctt tcattggcgg ccccgaaggc aatcgggaag cgcaggcccg gctcggcgcg
     2341 tatcgcaaag cgctcgagga gcacgggatc gcgttcgatc cacgcctcgt gacgactggt
     2401 gaattcacct ggccgagcgg cgcgagggcg ctgcggcaac tgctcgacga gcggcaagtt
     2461 cccttgtcat cgttggatag catcgtggcc gcgagcgact acaccgcgtt cggtgtgatc
     2521 gaggagctcg cccggcgacg catcgaggtg cccgagcacg tagccgtggt cggcttcgac
     2581 gatatgccgc gcgcgagcgt gcaccagccc ccactcacta ccgtaaagca gcagatcgac
     2641 gaactcgggc gcgaagccgc gcggatcgtg ctcggcttgc tcgagggacg cccggcagag
     2701 ccggtggtgc acttgacgac ggacttggtc ttgcgtcgct cgtgcggctg cacccccacc
     2761 gacgtgccga gcggtgacga ggacgcatcg agcgacgagc tcccccccgc agatcagcgc
     2821 atccagccgc gtggcggcgc ttggagcctc gagctcgtcg cggcgctcgc ggccgaagcc
     2881 agcggacaga tgaatgcgtt cgaaaaagcc ctcgatccac tgttgcagag gatgatcgta
     2941 gccggcgtcg acttgtcttt ggtccaagac atgatcttgt tcctcggtca gcgtgcgatt
     3001 cggcagctcg gcggcgactc gcgcttcacg caacgaatcg agagcgccgt tcagcgcggt
     3061 cgagtcctgg tgagcgaact cacatcgcgc gccgatcgca tgcccgagga ctcgatcagc
     3121 gagcagctgc acatcttctc gcgcgcgctc ttgtctcgga tgttcggccc gcagacgcag
     3181 ctatccgcgg tcttggtgga gcacttgccg cacttcggaa tcgacgaatg cctggtcagc
     3241 gagttcgtgc agccctcgag cgtggccgag ctgcgacccg ccttcgggtt cgacaaccgg
     3301 cacgtgcagc cgcagctcca ggcttacccg ggacgggagc tcgcgccccc agatttcgag
     3361 gggatgcacc atcactcggt cttcttgctc ccggtgcgct acggggaaca agcgataggc
     3421 atcgtgcttt tgcctccctg cgggcacgac ggcaccgtct acgagacgct ggcggaggtg
     3481 ttcggtatcg cgttgaaggg catcgagatg cgaaggaagg ccgaggcgcg ctgagctctc
     3541 gggctacgcc gcttgtgcga cgaggctctg gcgttcacaa tgcggcccca tgcgcgtcga
     3601 cgtttggacc gacttcatct gcccctggtg ttatctcggc aagcgccgcc tcgaactcgc
     3661 gctcgagcag ttcgcgggac gatcgacggt agagatccac tggcggagtt tccagctcga
     3721 tccggagttt ccgcgcgagt tctccggggg cgtgaacgac ctgctggtga acaaatatgg
     3781 catgagccgc gccgaggcgg aggccatcca cgcgagggtg acgggtctcg gcgccgccga
     3841 cggtctcgtg tatcgcttcg atcgctgccg gccgagcaat accttcgacg cgcaccgctt
     3901 gctccaggct gccaatcgtc gcggggtcgg ggcggccttg caagaccgct tcatgcgcgc
     3961 gtacttcgcg gagggcatct cgctcggcga cgcgccatct ctcagggcgc tggcgagcga
     4021 agccgggctc gaagcgccgg acagcgacgc cgtgttgagc ggcgacgcgt tcggtgagca
     4081 ggtttcggcc gaccagcgcc gcgcgcagga gctcggctat cgcggcgttc cggcgttctt
     4141 gttcgccgaa aaggttcagg tgtctggcgc gcagagcccg gagactctgc ttgcggcgct
     4201 tcagcgcgcg gagcgagcga gctcctgagc cgttcgcggt aagacgccgt ggtgacgcaa
     4261 ccaccgcctg agctgcggta cggtttcgtt gtaagggtcg atgcgctcgg cgcgccagcc
     4321 aatgtcgcgc gctccccgca cgttctcggg caagtcgtcg aagaacaaga tttccggacc
     4381 gcggagcgcg gtcgccgcct cgaatgcccg atagatctcg gcattgggtt tggcgaaacc
     4441 gaacaaatgc gaggcgtgcc gatggtggag gcggagcacg ctcgggtact cgggctcgcc
     4501 cgggagagcg ctcgtgccgt cgtgatgaac gagccgcgtc cagtgcgact gatcggtgtt
     4561 ggagagacag gcagtcgcga cgccaatcga gtcgagctcg tgcacgagct cgtgcgcgcc
     4621 ttcgtattct ccgagcgtcc aggcacgatg cactcggcga agctcatcgg cggtgtagag
     4681 gccgtcgagc gctcccgaga gctcgttcgc ccacgagagc tcgtcgagct tccccgtgtt
     4741 gaagcgctgt gagaattcgt aacgaatgcg ttgcgcgacg cgcccttcga tcgacgcgcg
     4801 aacgtcgagg cccgccgcgc ggcacgcttc gccgaagctc cggcagatgc gcaccaatac
     4861 gccacccaag tcgaagcaca cgagtttcac tgtcgggcta gcctgcattt cgctccgcga
     4921 atgctacgag ccggctcgga ttgcaacaag cacctccgtc aagccgaaag cacctgccaa
     4981 ggaattcgcg ccgtcacgct ggtgccccgg cccttcacgc tcgcgaccga gatgcttccg
     5041 gaatgcgctt cgacgatgcg ctttgcgagg gtcagtccaa ggccgacacc accggtgccg
     5101 cgtgaacggc tgcgctccgc gcggaagaag gcctcgaaga cgtgaggcag gtcgccttcc
     5161 tcgatgccca tcccgttgtc tttgacttgg aagacgaccg agctctgctc cttcgagacg
     5221 cagagctcga tcggggcgct ctcgtcgggt gagtattttt gggcgttctc gagcaagttg
     5281 tcgagaacgc ggcggagcaa caccgggtcc gcctcgatgg gcggcaagct gtcgtcgaag
     5341 cgggtcgtga acgggcgggt cgggtggcgc gcagcaaagc gttccgcgct gtccttcgcg
     5401 acgcgaagcg gcgagagctg ctcgacgtga agggccaggt gcgtgggagt cgccttcacg
     5461 tcggagagct cgaaacgcgt cgcgttcaag acgtcgtcaa tcaaggcctc gagctccgcg
     5521 agatcgaccg cgatttcgga gagcgagctc ttcacgattt cggcgtctcc ttcgctcgct
     5581 atttcgagcg cgacgcgaat acgcgagagc ggcgtgcgga gctcgtgggc gacgtttgcg
     5641 aggagctcct tttcggccgc gcgcaaagct tgaatgcggt cggccatgtt gtcgaaggcg
     5701 cgcgcgacgt ctccgatttc gtccgaccga gcgagccccg ttcgcgcgtt cagctcgccc
     5761 gacccaattg cggcggacgc tcgcgacagc tgctgcaacg gccccgcgat ccagcgcgcc
     5821 gtgagcagcg cgcccgctcc aacgaccacg agtcccgccg cgatggtgag ggagggcatc
     5881 cacaagctcg ggcgaggtcg ttcgaagcga gctacgagga cgccttcgcg cccctcgatc
     5941 gatatcggcg cgtacatgtg cggcggcacg tggccgttcg ggccatgtcc aaacgccggt
     6001 acgagcggcg gtgagggtgg gggcccgttc ccgcgcgggg gtgcagcgag gggcgcagga
     6061 tcgaggcggg gcgagagccg aggcgggtgc aggggcggct cgacgttgct ggccaggagg
     6121 tgccggtctt cgtcgtacaa cgaaagcagc aacccgtgcc ggcgccgcag catctcgagc
     6181 tcaccgccga gcgcgtggcg gtccaagacg actcgagtca gctccgcggt gatgctttgc
     6241 atgtcccagc gcggcggtgt cttcgccatg gcgtaggcga tgccgaccgc ggcgaccgcg
     6301 accaagaaga gctgtacgac gccgatcgcg tacagccgat acaccaagcg cgaacgcagc
     6361 atcatggcgg ctcgggcgcg agcatgtagc ctacgcctcg gatggtcttc agcagtcgag
     6421 gattgcgggg atcgtcttcg agcttgtggc ggaggcgtga gacgtggacg tcaatcgagc
     6481 gatcgaatgc ctcctccgcc gtcccgtgga ggagtgagag cagttgctcg cggccgagca
     6541 cgcggccggc gcgctcggcg agcacgcgga gcagtgaaaa ctcgtaggtc gtgagcgaca
     6601 ggcgactgcc gttcagcgtc gcggacatgg ttccggcgtc gaccacgagg gggccgacct
     6661 cgatgcgctc tggcgtcggc ccagcgcgtc cccgggcgcg tcgcgcgtga gcacggatgc
     6721 gagccaagag ctctcgcgaa gagaacggct tgctaacgta gtcgtcggcg ccaccttcga
     6781 gcccgagcac gcgatcagcc tcgtcggtgc gggccgtgac catgatgatg gggacgtcca
     6841 tgcgctcgcg cactcggcga cagacctcga ggccgtccgc gccggggagc atcaggtcga
     6901 gcagcacgac gtcgggtcgc acccgagtga tctcggcgaa tgcgacttcg ccgctcggta
     6961 cgatcgtcac gtcgactcca tggcttctca agtactgcgc cgtgagccgc gcgagtcgcg
     7021 cgtcgtcttc gacgtacacg acggtcaaga gctcgtagct cgacgccgct tcagggctga
     7081 ccatccgtca cacctcgtga acgaaaaatt gtaccgctcg acaatgcatc gagggaaacc
     7141 atgacacgca cgccgacggc tgcccgacgt cgctccccgg ggacgcgaga aagctcgcga
     7201 acgtagcctg tttcgatttg ttaagaggcg ctacggtgtt tcggctcgct tgccgatgca
     7261 gccctcgacg gcggtgttcg gcgcggctgc cttgcagccc gcctcgctga agaaccatcg
     7321 accgtgctcg aactcgagtg agtccgcgtt cacggccgtg agcttcaggt tctgatcgca
     7381 ggtccactct cgctcgtcgg tcggtttgct cgcgacgccg ggcgggatcg ggccgaacac
     7441 tcggatctcc tccgccccct gccagaagtg aaacgcgcgg gcgccgtgag cttgcggcgc
     7501 gacttcgcga agcgtgccca tcggtgcgcc gggcgagagc ggttcgaagc ggaacgcctc
     7561 gcatacgtcg ctcccggcaa ccttccaata cagtgtggcc ttgctcttcg tgagctgagc
     7621 gagagtctca ccgggcagag cattgcaaaa cgcgttgagc aggcgctctt cggtcaggac
     7681 cttctcgaag gcgagcgcgt ctcgttgcca gccacccgaa aactcttcgt cgaagcgctc
     7741 catctgctcc aacagcttgc cgacggagcg cagcggcatg ctggctccgc agcgctcgtc
     7801 gcgaatgaag ccgaacggca gcgccgcgag cgtcgcggtt ccggcgcgtt gctcggccac
     7861 gacgatgggc ttgagactcg gcgcgatcag atcactgcag cggcagagag gtagctcttt
     7921 tcggagttcg tttcgcagcg tttgccaacg ctgttcgacg ggagctgcgt cggcgacgct
     7981 cagcgctcga gacaacccgc tacatcgaga gaactgcgaa aaagct
//
LOCUS       DASTKS010000836         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1473254, whole genome shotgun sequence.
ACCESSION   DASTKS010000836 DASTKS010000000
VERSION     DASTKS010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473254"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..130
                     /locus_tag="VFQ35_17255"
     tRNA            54..130
                     /locus_tag="VFQ35_17255"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Met,seq:cat)
     gene            complement(109..1809)
                     /locus_tag="VFQ35_17260"
     CDS             complement(109..1809)
                     /locus_tag="VFQ35_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9932457.1"
                     /translation="MTSRPTQHFPMQTSEAQIPAVIYCRYSTDHQDSRSIEDQTRRCR
                     AYAAAHGFRVVAEHSDAATSGSHVERAGMQQLLASARAPGGAPFRVVLVDDLSRLSRD
                     LGDTWQIVFRDLASANVSVIDVTTGLASNATGGRLVFGALALVNDTFLQLVRTETHRG
                     LEGRALGGFSTGGRCYGYATVQEENPPDPEHPRKRFLIDRNEADVVLRVFREFAAGMS
                     LKKVADMLNRDGVVAPHDGGRGNKVGRGWGHTTIRAMLRNERYVGRVTWNQSKWVQVP
                     GRKSRRRVPRPEAEWVTQTIPELAVVSPELWDAANARFQRVHAYARGRPAGTGKHVYL
                     VSGLLRCGVCGGSMTVIGRKAKAGVSYARFGCTAHASRGSSICPNALSLSEKKASRTL
                     VSALREKLGRPELVERFVATFRRRVAERRVEASATTDMNRLVRECERRLSNLTDALAK
                     VGWSEAIATKLREEEALLGKLKAERTAATRADGASVIPHPAVIAGYLENLLALLETDP
                     VRGRELLSRFISPVVMTPEAEGPERRYRATGAFNLSFILTAAASSESRSGKYGCAGRI
                     "
     gene            complement(2017..>3299)
                     /locus_tag="VFQ35_17265"
     CDS             complement(2017..>3299)
                     /locus_tag="VFQ35_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932458.1"
                     /translation="RVDALAVAFRVELSGSFADELCERQALADVGGGAELKLPSGIAL
                     ALRRSRRQSFYAFQNADVRGAVDLLASGGWTLEVVLRAAFLATHPLSAALAVAERVAV
                     GFGEAHERRLRRFDLAADFVGWPLRRDDAERFSTRAGKASFLVDSKDLDEVETLLVKP
                     EIREFSRNGVGVTGLTFAAGGDLSARVYAKSFELSLPGREAKREIEHALWRAAGWCGQ
                     EVTRAEFQHRGPFLDEINLRDPYALEPNLDAIWAYDTQRFLRMKVPGTASRVERCADD
                     PRWVATRGAFRHDVSPIARTRALRGGASAAQVVGATLSHSAAAGSLERAPHLERVTQR
                     DELCDAFEDAIETIGGYDRVYNEVLRRTAPANARASAEFAARLVLRHGPDALKVFFGR
                     VNGLNARFWSVDDERATCGVPNGLDDSVKGASDK"
BASE COUNT          603 a         1076 c         1049 g          571 t
ORIGIN      
        1 cgtacgtctt ggttgacaat gacgtcttcg cgactaaggt acccgctctc tgacgcgcgg
       61 tggagcagcc tggtagctcg tcgggctcat aacccgaagg tcggaggttc aaatcctccc
      121 cgcgcaacca tactttcctg accgactctc gcttgaagcg gcagcggtca ggatgaacga
      181 gaggttgaag gccccggtcg cacgatagcg acgctcgggg ccttcggctt ctggggtcat
      241 gacaactggt gagatgaagc gcgagaggag ctcgcggcct cgcaccgggt cggtctcgag
      301 cagcgcgaga aggttttcga ggtagccggc aatgaccgca gggtggggga tgacgctggc
      361 gccgtcggct ctagtcgcag ccgttcgctc cgctttgagc ttcccgagca gcgcctcctc
      421 ttcccgcagc tttgtcgcta tcgcctcgga ccaaccgact ttggccaggg cgtccgtgag
      481 gttcgagagt cggcgctcgc actcgcggac gagacggttc atgtccgttg tagctgacgc
      541 ttcgacccgg cgctcagcca cacgccgccg gaaagtagcg acgaaccgct cgacgagctc
      601 ggggcggcca agcttctcgc ggagcgcgct cacgagcgtg cgcgacgctt tcttctctga
      661 cagcgacagc gcgttcgggc agatcgacga cccgcgtgat gcgtgcgccg tgcagccgaa
      721 ccgagcgtat gacacgccgg ctttcgcctt gcgaccgatg acggtcatgc tgccaccgca
      781 tacaccgcag cgaagcagcc cagacacgag gtacacgtgc ttgccggttc cagctgggcg
      841 cccacgcgcg taagcgtgca cgcgctggaa gcgagcgttt gctgcgtccc aaagttccgg
      901 ggaaacgacg gcgagctcgg ggatcgtttg ggtgacccat tctgcttccg ggcgcggcac
      961 gcggcgacgg ctcttgcgac cgggcacttg cacccacttg gactgattcc aagtcactcg
     1021 gccgacgtag cgctcgttgc ggagcatcgc gcggatcgtc gtatgacccc atcctcgccc
     1081 gaccttgttg cctcgccctc catcgtgcgg agccacgacc ccgtcccggt tgagcatgtc
     1141 ggcgaccttc ttgagcgaca taccggccgc gaattcccgg aacactctga gaaccacgtc
     1201 cgcctcgttc ctatcgatga ggaagcgctt tcgcgggtgc tcggggtcgg gcggattctc
     1261 ttcctggacg gtcgcatagc cgtagcatcg gccccccgtc gaaaagccgc ccagcgcgcg
     1321 cccttcgagg ccgcggtgcg tctcggtgcg gacgagctgc agaaacgtat cgtttacgag
     1381 cgcgagcgcg ccgaaaacca agcgtccgcc ggtcgcgttg gaagcaagac cagtcgttac
     1441 gtcgatgacg ctgacattgg ctgaagcgag atcgcggaag acgatctgcc acgtgtcccc
     1501 taggtcgcgc gagaggcgag aaaggtcgtc gactagaacg acgcgaaacg gagcgccgcc
     1561 cggagcgcgc gcggacgcga gcaactgctg catgcccgcg cgttcgacat gggagccaga
     1621 cgtcgcggcg tcgctgtgct ctgcgacgac tcgaaatcca tgcgcagcag cgtacgcccg
     1681 acaacggcgc gtttggtcct cgatcgagcg agagtcttgg tgatccgtcg agtatctgca
     1741 gtagatcaca gccggaattt gagcttcact cgtctgcatc gggaagtgct gcgtcggtcg
     1801 ggaagtcatg aaggcacccc tccacccaga ggtcggcggc gaggcgggcg agtgcgtcca
     1861 cgaagtccaa catagctgag agatcctggg gtggccggtc tgtcccggtg accacgggcg
     1921 atctggcctg gagagtgagc aattggccgc cgtccggtgc tccacgggct tcagctcgcg
     1981 ttgggtcccg cggatgctca gggccaacgg actgcttcac ttgtccgacg cccctttcac
     2041 agagtcgtcc agcccgttgg ggacgccgca ggtcgcgcgc tcgtcgtcca cgctccaaaa
     2101 acgtgcgttc aacccgttga cgcgtccaaa gaaaaccttg agggcgtctg ggccgtggcg
     2161 aagcacgagc cgagcagcga actctgctga agcacgcgcg tttgcaggcg cggtgcgccg
     2221 aagcacctcg ttgtacacac gatcatagcc tccgatcgtt tcgatggcat cttcgaacgc
     2281 gtcacacagc tcatcgcgct gcgtgaccct ctcaaggtgg ggtgcacgct cgaggctgcc
     2341 tgctgccgcg gaatgcgaca gcgttgcacc gacaacttgc gccgcagatg caccgccacg
     2401 cagcgcacgg gtccgcgcga tcggcgaaac gtcatgacgg aacgcgccac gggtagcgac
     2461 ccaacgcgga tcatcggcgc atcgctcaac gcgcgacgcc gtgcccggca ccttcattcg
     2521 caggaaacgc tgtgtgtcat aggcccaaat cgcgtcgaga ttgggctcga gggcataggg
     2581 atcacgaagg ttgatctcgt ctaggaacgg tccgcgatgc tgaaattctg cgcgtgtaac
     2641 ttcctggcca caccacccgg ccgcccgcca aagagcatgc tcgatctccc gcttcgcttc
     2701 gcgaccaggc aagctcagtt cgaagctttt tgcgtagacg cgcgcggata gatcgccgcc
     2761 agcagcgaat gtcagaccgg tgacgccaac gccgtttcga ctgaactccc ggatctcggg
     2821 cttgaccaag agggtctcga cttcatcgag gtccttgctg tcgacaagaa agctcgcctt
     2881 gccggctctc gtcgagaatc gttctgcatc gtcccgtcgg agtggccagc ccacgaagtc
     2941 agccgcgagg tcgaaccgtc gcaatcgacg ctcgtgtgcc tcgccaaaac caacggcgac
     3001 gcgctcggcc accgcgagcg ctgctgacaa agggtgcgtc gcgaggaagg ccgcacgcaa
     3061 cacgacttca agcgtccagc caccggatgc caagaggtcg acagcgccac ggacgtcggc
     3121 gttttgaaac gcgtagaagc tctggcggcg actgcgccta agtgccaggg cgattccgct
     3181 aggcagcttc agctcggcgc cgccgcccac gtccgcaagc gcttgccgct cgcaaagttc
     3241 gtccgcaaac gacccggaca gttcaactcg aaatgcaacg gcgagcgcgt cgacgcggc
//
LOCUS       DASTKS010000837         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1250045, whole genome shotgun sequence.
ACCESSION   DASTKS010000837 DASTKS010000000
VERSION     DASTKS010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250045"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFQ35_17270"
     CDS             <1..275
                     /locus_tag="VFQ35_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET9932459.1"
                     /translation="AGGTVIGVDFRTPIDQAIRRLGDGVAVQGNLDPLLLDAPRPLLT
                     RRIDEILGAVGGRNGHVFNLGHGILPETSPDAVKFVVEYVHERTAD"
     gene            377..450
                     /locus_tag="VFQ35_17275"
     tRNA            377..450
                     /locus_tag="VFQ35_17275"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:409..411,aa:Gln,seq:ttg)
     gene            693..1301
                     /locus_tag="VFQ35_17280"
     CDS             693..1301
                     /locus_tag="VFQ35_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932460.1"
                     /translation="MIELDDAAERRFIERLQERDERAFIELVQLYQVRVYKLVVRMLG
                     RRDEAEDMAQEVFVQVFKAVGTFRGDSRLSTWVYRIAVNLCKNRIKYLGRRRSDAQDE
                     FEATEDRSEHSGAPGVTSGEAARPDQLVEGYQMQRVVERAIAEMDPDFREILVLRDIE
                     DLTYEELCEITGLPEGTVKSRLHRARAMLKSAIERKMGEKVG"
     gene            1298..1639
                     /locus_tag="VFQ35_17285"
     CDS             1298..1639
                     /locus_tag="VFQ35_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932461.1"
                     /translation="MSHESDEEARSSVPELDSSAEQSMRDLLRGTLGERHSAPDLVAG
                     VQQKIRVRSGGKFYADGWSTVRHPPLNTYLISSLLMLFVLCIVYALLAPLSGAPEHPK
                     PSAPVQVLPQP"
     gene            1977..>2404
                     /locus_tag="VFQ35_17290"
     CDS             1977..>2404
                     /locus_tag="VFQ35_17290"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932462.1"
                     /translation="MLRKVSLLENWQFSAKSWLDAPNGMHGFSKLEWLPARIPGHVHL
                     DLVGNGVIAHPFENLNELGARWVDAEDWVYRCEFDFAFDAERPQTRLYFGGLDTVATI
                     YLNGKQIGEHDNMFVPCEFDVGTALVAGKNLLEIQFASAER"
BASE COUNT          447 a          741 c          787 g          429 t
ORIGIN      
        1 aggcgggcgg caccgtcatc ggtgtagact ttcggacgcc catcgatcag gccatccgcc
       61 gcttgggtga cggagtcgcc gtgcagggca acctcgatcc gttgttgctc gacgcgccgc
      121 gcccgctgct cacccgccgc atcgacgaga tcctcggcgc cgttggcggg cggaacggcc
      181 acgtcttcaa tttgggtcat ggcatcttgc cggagacgtc gcccgacgcc gtgaagttcg
      241 tcgtcgagta cgtgcacgag cgcacggctg actgaccgag cggccccggg gccagcgcgc
      301 cggggcgtgc ccgaagcgtt ctttccctga gagcgccgtt gatttgagaa cgagagtggc
      361 taagctctcg cgcctttccc gcgtcgtcta aaggcaggac aagggacttt gaatccctga
      421 atcgtggttc gaatccatgc gcgggaacca cactgggcca tcgctgagtc ggagcgtggc
      481 cctcgcccgc caaggcgccg gtcagccctc gtgcgcttgg gtgcgacagc cgcgccatcg
      541 cggggcggga actttcgtag gcgcgtcgct gtcgatcgaa aaaggacggt gcgcggcgac
      601 gatatcgcga gaagatcgca gcgggcccga ctcaccgggt tcttgctcaa cgtgacgaag
      661 gcgaagcgta accaagtcct gtgcgggggt tcgtgatcga gctcgacgac gccgcagagc
      721 gccgcttcat cgagcggctg caagagcgtg acgagcgcgc cttcatcgag ctcgtgcagc
      781 tctaccaggt gcgcgtttac aaactcgtgg tgcgtatgct cgggcgccgc gacgaagccg
      841 aggacatggc gcaggaagtc ttcgtgcaag tcttcaaggc agtcggcaca tttcgcggcg
      901 actcgcgcct cagcacctgg gtttatcgca tcgcggtgaa cctctgcaaa aatcgcatca
      961 aatatttggg ccgccgccgc agcgacgcgc aggacgagtt cgaggccacc gaagatcgca
     1021 gcgaacactc gggcgcgccg ggggtcacga gcggcgaagc ggcgcgcccc gaccagctcg
     1081 tcgagggcta ccagatgcag cgcgtcgtgg agcgcgcgat cgccgaaatg gatcccgact
     1141 tcagagaaat cctcgtgctg cgcgacatcg aagacctcac gtacgaagag ctctgcgaga
     1201 tcaccggctt acccgaaggc acggtcaaga gccgcctgca ccgcgcgcgc gccatgctga
     1261 agagcgcgat cgagcgtaag atgggagaga aggtcggatg agccacgaga gcgacgaaga
     1321 ggcacggtcg agcgtgcccg agctcgattc gagcgcggag cagtccatgc gcgatctgct
     1381 gcgcgggacg ctcggtgagc gtcactcagc gccggacttg gtcgccggtg tgcagcagaa
     1441 gattcgcgtg cgaagcggcg gcaagttcta cgcagacggc tggagcacgg ttcgccatcc
     1501 gccgctcaac acgtatctca tcagcagcct gttgatgctc ttcgttctgt gcatcgttta
     1561 cgcgctgctc gcgccgctct ccggcgcgcc cgaacacccc aaaccaagcg caccggttca
     1621 agtccttccg cagccgtaac ggaaacgagc gcttctttcc aacgccaaga ccccaaggag
     1681 cgccaaggcc gcggagattt tgggggtgaa cattgcgaac ccgttttgtc tgcttcggct
     1741 tcgcagagtg atcgtcgttg cggactgatc gtcgtcgcgc gggcccgagc aattgatttc
     1801 gcagagtggt cagtgcgaga acgaaacgtc tccgttccgg cgctcccccc aagaaaaata
     1861 ttctgcggcc ttggcccccc tcggcgcctt ggcattgaag aagcgcccgt ttcggtcccg
     1921 gagcgcctgg gaaggaaaga ttgtgacttg aggggcagag cgttagcgtg cgccgcgtgt
     1981 tgcgaaaagt ctctctcctc gaaaactggc aattctccgc caagagctgg ctcgatgctc
     2041 cgaatgggat gcacggcttc agcaagctcg agtggttgcc agcgcgcatt ccggggcacg
     2101 ttcacctgga tctcgtcggg aacggtgtca tcgctcaccc gttcgagaac ttgaacgagc
     2161 tcggtgcgcg ctgggtcgac gcggaagatt gggtataccg ttgcgagttc gacttcgcgt
     2221 tcgacgccga gcgcccgcaa acgcgcctgt atttcggtgg gctcgacacg gtcgcgacga
     2281 tttatctgaa cgggaagcag atcggtgagc acgacaacat gttcgtgccc tgcgagttcg
     2341 acgtcggcac cgcgctcgtg gccggcaaga acctgctgga gattcagttt gcttcggcag
     2401 agcg
//
LOCUS       DASTKS010000838         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_802659, whole genome shotgun sequence.
ACCESSION   DASTKS010000838 DASTKS010000000
VERSION     DASTKS010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802659"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1825)
                     /locus_tag="VFQ35_17295"
     CDS             complement(242..1825)
                     /locus_tag="VFQ35_17295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932463.1"
                     /translation="MNAIATVKVRRSVVTRVLWSYAFVVLAFSLVAGWSALGLRRAAD
                     EAALMRSGYYPLARAVRDLAAKQDTYNSQLNHITAARNPADLRVWFDFALKVGRPKMF
                     AQVRAAIGRAFSSSGDADTRAVGKALLVDVASIDRAMASDAELLARLFDALERRDDIG
                     AERLRDDLVTRGTQGSVRLSRLEERVEVEIDRLLDQARGREYLSIQLLIGLSGLSLLV
                     GLLMALYARRVLRPLSAVTERAKAVARGDLEPHPPIVSGDEIGELSATFESMVHAIAL
                     ANERLVANERLAAVGKMAAHVTHEIRNPLSSIALNLELLEEELESSSGESRALVRAIG
                     SEVDRLNQLSQQYLSFARQKSSNLIEEDLVQVVREASEFVRRELRQSDVTLELSLDDS
                     VGPVPIDEGQIKQVLLNLLRNARDAMPQGGRVSVSVRPAGDKAEVSVEDEGQGLDADA
                     RARLFEPFFTTKRHGTGLGLAITRQIVEAHGGSIEFTDRAPRGTKVTLLLPRAPEKHR
                     TPDAPNAENKDRTEALEPT"
     gene            complement(1822..2889)
                     /locus_tag="VFQ35_17300"
     CDS             complement(1822..2889)
                     /locus_tag="VFQ35_17300"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9932464.1"
                     /translation="MKSPHRLNVLSNRQPELETCAYCPKLCRAACPIAEVEASETVTP
                     WGQMRAVFDVARGAIEPSADFAALSWACSSCRNCRESCDQRNLVPETLFEARALYREQ
                     GLAPPSIARFHERFGERLLRVKARIEELRARHHSATAPTALVLGCEYALHLPGEIEDG
                     IAAAQSLFGAVRIVSGCCGLPLDAAGDPVGARTLRAEMKSELGDARRKLAFDAGCAFT
                     LRDQGVEPFARAAEAALAGVRTEPTTTGSLRYHDPCYLARGLGETRAPRALLERASGR
                     PPAEFSRRENRTYCSGGGGLLPLSRPETRARIAANRLEEHERLGGGTIVTACAASLRS
                     LRTAGASVVDLMTIVRQLSNR"
     gene            complement(2886..>3742)
                     /locus_tag="VFQ35_17305"
     CDS             complement(2886..>3742)
                     /locus_tag="VFQ35_17305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9932465.1"
                     /translation="ALYNPEGSRPFDAEDQAMLRLVAANVSTAVRLFNANAAREREER
                     LTSIGRLLSQVIHDFKSPMTVISGCVQLMAETDDRGKRQQYTDEILKQFDVLGAMQRE
                     VLEFARGERTIFVRRVYMRKFFADLAKQIALEIDGKPIELEVEVDSKLVARFDEGRVA
                     RAIHNLARNSIEAMGERGGRLSIKAAIERSDLVIRVADTGPGVPKEIEGRLFQSFVTA
                     GKKDGTGLGLAIVKKIADEHGGRVSVSSSPEGATFEIALPQKSEQPASRPRKSTRPPP
                     RAQSSKPE"
BASE COUNT          589 a         1208 c         1317 g          628 t
ORIGIN      
        1 cgacgtccta gacggaggcg atgccaccct aatctttcac gacgacgaca tcttctgggg
       61 tcattacatc acagtcgggc tcagagtcga cgggacgttc acttgcaaag gcatcgtggg
      121 ctgacgtcct ggccacgagc gcttcggagc gctcacgggg gcacctgggt caccaaagca
      181 tgcgctcacc agtctgcttc tgagaaacgg atgcgacgac gagaagcatc actttccgct
      241 ctcacgtcgg ctcgagcgct tccgtgcggt ccttgttctc cgcgttcggt gcgtcgggag
      301 tacggtgttt ttccggtgcg cgcgggagca ggagggtgac tttggtgccg cgcggtgcac
      361 gatcggtgaa ttcgatgctg ccaccgtggg cttcgacgat ttggcgggtg atggcgaggc
      421 cgaggccggt gccgtggcgc ttggtggtga agaagggctc gaagaggcgc gcacgcgcgt
      481 cggcgtcgag gccttggcct tcgtcttcga cggagacttc ggccttgtcg cccgcggggc
      541 gcacggagac ggagacgcgg ccaccttggg gcatggcgtc gcgcgcgttg cgcaggaggt
      601 tgaggaggac ttgtttgatt tggccttcgt cgatggggac ggggccgacg ctgtcgtcga
      661 gcgagagctc gagcgtgacg tcgctttgac ggagctcgcg gcgaacgaac tcggaggctt
      721 cacgcacgac ttggacgagg tcttcttcga tcaggttgct cgacttttgg cgcgcgaatg
      781 agaggtattg ctggctgagc tggttcaggc ggtcgacttc gctgccaatg gcgcgcacga
      841 gggcgcgtga ctcgccggag ctcgattcga gctcttcttc caagagctcg aggttgagcg
      901 caatcgacga aagggggttt ctgatctcgt gggtgacgtg cgcggccatt ttgccgacgg
      961 cggcgaggcg ctcgttcgcg accaggcgct cgttggcgag ggcgatggcg tgcaccatgc
     1021 tctcgaaggt ggcggagagc tcgccgattt cgtcgcccga gacgatcggg gggtgaggtt
     1081 cgaggtcacc gcgagccacc gctttggcgc gctcggtgac ggccgagagc gggcggagca
     1141 cgcggcgcgc gtagagcgcc atcaagagcc ccacgagcag gctcaagccg gaaaggccga
     1201 tcaggagctg aatcgagagg tactcgcggc cgcgagcctg atcgagcaag cggtcgattt
     1261 cgacctcgac gcgctcttcg aggcgcgaga ggcgaacgga gccttgggtg ccgcgcgtca
     1321 cgagatcgtc ccgcaggcgc tcggcgccga tgtcgtcgcg ccgctcgaga gcgtcgaaca
     1381 agcgcgccaa gagctcggcg tcgctcgcca tggcgcgatc gatgctggcg acgtcgacga
     1441 gcagcgcttt gccgaccgcg cgcgtgtcgg cgtcgccgga gctcgagaag gcgcgaccga
     1501 tggcggcacg cacctgagcg aacatcttgg ggcggccgac cttgagcgcg aagtcgaacc
     1561 agacgcgcag gtccgccggg ttccgagccg cggtgatgtg gttcagctgc gagttgtagg
     1621 tgtcttgttt ggccgcgaga tcgcgcacgg cgcgcgcgag cggatagtag ccgctgcgca
     1681 tgagggcggc ctcgtccgcg gcgcggcgca agccgagcgc gctccagccc gccacgagcg
     1741 aaaaggcgag caccacgaag gcgtacgacc acaacacgcg cgtgacgacg ctgcggcgaa
     1801 ccttcacggt cgcgatggcg ttcatcgatt ggagagctgg cgaacgatcg tcatcagatc
     1861 cacgacgctc gcgcccgccg ttcgcaagct ccggagcgac gctgcgcatg cggtcacgat
     1921 ggtaccaccg cccagccgct cgtgctcttc gaggcggttc gcggcgatgc gagcgcgcgt
     1981 ctccggccgt gacaaaggca agaggccgcc tccgccggaa caatacgtgc ggttttcccg
     2041 gcgcgaaaac tcggctggcg gccgaccgct ggcgcgttcg agcagcgcgc gcggcgctcg
     2101 agtctcacca aggcctcgcg ccaggtagca ggggtcgtga tagcgcaagc tcccggtcgt
     2161 cgtgggttcg gtgcgcacgc cggcaagcgc cgcctcggcg gcccgcgcaa agggctcgac
     2221 cccctggtct ctcagggtaa aggcgcagcc cgcgtcgaag gcgagcttgc gccgcgcgtc
     2281 gccgagctcg ctcttcatct cggcgcgcag cgtccgcgcc ccaacggggt cgcccgcggc
     2341 gtcgagcggc agcccgcagc atcccgacac gatgcgcacc gcgccgaaca acgactgcgc
     2401 ggccgcgatg ccgtcttcga tttcgccggg aaggtgcaga gcgtattcgc agccgagcac
     2461 gagcgcagtc ggcgcggtcg ccgagtgatg gcgggcgcga agctcctcga tgcgtgcctt
     2521 cacgcgcaga aggcgctcgc caaagcgctc gtgaaagcgc gcgatgctcg gcggggcgag
     2581 gccctgctct cgatacagcg cgcgcgcctc gaacagcgtt tcgggcacca ggttgcgctg
     2641 gtcgcagctc tcacgacagt ttcggcacga gctgcacgcc cagctcaacg ccgcgaagtc
     2701 ggcgctcggc tcgatggcgc cgcgcgcgac gtcgaacacc gcgcgcatct gtccccaggg
     2761 cgtcacggtt tcgctcgcct cgacttcggc gatgggacac gcggcgcgac acagcttggg
     2821 gcaatacgcg cacgtctcga gctccggctg ccgattcgag agcacgttca ggcgatgcgg
     2881 agatttcatt ccggcttcga cgactgcgcg cgcgggggag gccgagtcga cttccgaggc
     2941 ctcgaggcgg gctgttcgct cttctgcggc agcgcgatct cgaacgtcgc gccctcgggc
     3001 gaggagctca cgctgactcg gccgccgtgc tcgtcggcga tcttcttcac gatcgcgagg
     3061 ccgagcccgg tgccgtcttt cttgcccgcc gtgacgaacg actggaacag gcggccctcg
     3121 atttctttgg gtacgcccgg cccggtgtcg gcgacgcgaa tcaccaaatc cgagcgctcg
     3181 atggcggcct tgatgctgag cctgccgccg cgctcgccca tcgcctcgat ggagttgcgc
     3241 gccaggttgt ggatggcgcg cgcgacgcga ccttcatcga agcgcgccac gagcttggag
     3301 tcgacctcga cctcgagctc gatgggcttg ccgtcgatct cgagcgcgat ctgcttcgcg
     3361 agatccgcga agaactttcg catgtacacg cgccgcacga agatggtgcg ctcgccgcgc
     3421 gcgaactcga gcacctcgcg ctgcatggcg ccgaggacgt cgaactgctt caagatctcg
     3481 tcggtgtact gctggcgctt gccgcgatcg tccgtctccg ccatcagctg cacgcaaccg
     3541 ctgatcaccg tcatcggcga cttgaagtcg tgaatcacct gtgacagcaa acgaccgatc
     3601 gacgtgagcc gctcttcgcg ctcacgcgcc gcgttcgcgt tgaagagccg caccgccgtc
     3661 gagacgttcg cggcaacgag gcgcagcatc gcttggtctt cggcgtcgaa cggccgcgac
     3721 ccttcggggt tgtagagcgc ga
//
LOCUS       DASTKS010000839         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1025828, whole genome shotgun sequence.
ACCESSION   DASTKS010000839 DASTKS010000000
VERSION     DASTKS010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025828"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFQ35_17310"
     CDS             <1..1363
                     /locus_tag="VFQ35_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932466.1"
                     /translation="LMSLGERGGEAAPAFGIGWAAAVRSLVRFSVALLFAGLLGVLFV
                     RLEIGTRRVGRLLAPPLGALGASLPAVFLARAFGANQALWPRETLAVAVTSFVGAALL
                     SRLVLSLAERAASRPSNAFLFRTELSALPSLLPWLTSALFSTELALDLDGVAESVLTA
                     LSRSEVSAGVTCAVVCAALTAALVSFNATRPLVSLDSGPTLIAVDSDKRRRMLWRGGF
                     VVLLLVGYSVLSLKRSGGGSDLALLSQGARALLYYGLVTLVVSTVLGISLGALAASGP
                     VALDRLFIVVVEIGASLPNMLWAAALSRAAGSGLVFAIALGCLRAPDLAWLYRAELMR
                     LAAADSELGARSLGRLPLSIYLRSRLWPAAALVMRGAALTPAWALGIAASAEVAGFSA
                     SAGEPGWERLFALATPARVPFVVLAALGLAAATWFLVWAFGGAPRRVGIRSTRPPPPL
                     PNG"
     gene            1437..2078
                     /gene="trxA"
                     /locus_tag="VFQ35_17315"
     CDS             1437..2078
                     /gene="trxA"
                     /locus_tag="VFQ35_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9932467.1"
                     /translation="MALQTVSERDFEQEIMMSELPVLVEFSAEWCGPCKVVAPELEAL
                     SRELDGKAKIVKIDIDRSPMIARQLGVQSVPTFVVFQNGRPVGGKVGALKRNQLREML
                     DPFLPRAEGALKPEELAALLKAGRVSVVDAREPAVYGRAHIQGAVNIPGSEIEGRLAE
                     LHMLPMPPVIYCRSGDLTKELAAKLAEQGVPVAFLEGGVLGWEASGFTLHRPD"
     gene            complement(2106..>3060)
                     /gene="larC"
                     /locus_tag="VFQ35_17320"
     CDS             complement(2106..>3060)
                     /gene="larC"
                     /locus_tag="VFQ35_17320"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET9932468.1"
                     /translation="AQIDALIAESDLPQGTAELARRIFARLAKAEAEVHRVPLESVSF
                     HEVGSVDAIVDIVGAAACFEYLGARVLATPLPMGRGFVRCQHGRLPLPAPATLLCLSG
                     VPTYDAGIEGELVTPTGAAIVATVAEAFLSWPRFVPERTGFGCGTRKLPDRPNALRAV
                     LGAPFAVAASEAASHVVLEANIDDMTGELAAHAISVLLSEGALDAWAQPITMKKGRPG
                     LTLSVLAASDRADALAAAMLRETSSIGVRRTQVSRDERPRRVIEIETRYGRVPVKVSE
                     GDYGPPQRKPEFDVCANLARAAGVPVREVIAAALAALEKKS"
BASE COUNT          415 a         1065 c         1018 g          562 t
ORIGIN      
        1 gctgatgtcg ctcggcgagc gaggcggcga ggcggccccg gcgttcggca tcgggtgggc
       61 cgccgcggtc cgtagcttgg tccgcttctc tgttgcgctt ctgttcgcgg gcctgctcgg
      121 ggtcctgttc gtgcggctcg aaatcggaac gcggcgcgtt ggccgattgt tggcgccgcc
      181 gctcggagcc ctgggcgcga gtttgccggc cgtatttctg gcgcgcgcgt tcggtgcgaa
      241 ccaagcgctc tggccgcgcg aaacgctggc cgtcgccgtc acgagcttcg tgggcgccgc
      301 gctgttgtct cgactcgtcc tctctctcgc cgagcgcgcc gcttcgaggc ccagcaacgc
      361 ctttttgttc cgcaccgagc tcagcgcgct gccgagcctt ctgccgtggc tcacgagcgc
      421 gctcttcagc acggagctcg cgttggacct cgacggcgtc gccgagagcg ttctcaccgc
      481 gctttcgcgg agcgaagtca gtgccggcgt cacctgcgcc gtggtctgtg ctgccctcac
      541 cgccgctctg gtgtcgttca acgcgacgcg acccctcgtc tcgctcgatt ctgggccaac
      601 cctcatcgcc gtcgacagtg acaagcggcg ccgcatgttg tggcgcggcg ggttcgtcgt
      661 gctgcttctc gtcggctact cggtgctgtc gctgaagcgc tcgggaggtg gcagcgacct
      721 cgcgttgttg tcccagggag cgcgggctct cctttactac gggctcgtta cgctcgtcgt
      781 ctcgacggtc ctcgggatct cgctcggtgc actcgcggcg agtggaccag tcgcgctcga
      841 ccgcttgttc atcgtcgtcg tcgaaatcgg cgcgtcactt ccaaacatgc tgtgggctgc
      901 agcgctctct cgcgccgcag ggagcgggct cgtctttgcg attgcactcg gctgcctccg
      961 tgcgccggat ctcgcgtggc tgtatcgagc cgagctgatg cgactcgccg ccgctgactc
     1021 cgagcttggc gcgcggtctc tcggtcggtt gccgctctcg atctacctac gcagccggct
     1081 gtggcccgct gcagccctcg tcatgagggg tgcggcgctc acgccggcct gggcgctcgg
     1141 aatcgcggca tcggcagagg tggccggctt ttccgcctcg gctggagaac caggatggga
     1201 gcgtctgttc gccctcgcga ccccggcccg agtcccgttc gtggtgctgg ccgccttggg
     1261 gctcgccgcg gcgacctggt tcttggtgtg ggcatttggc ggggctccac gccgcgttgg
     1321 catccgctcg acccgtccgc cgccgccgct tccgaacggc tgacgccgac cagtgcgaag
     1381 aactgcccga gaggagggct tgcgcatcac gcaggaatgt tcgaaatact cgtcgcatgg
     1441 cgctccagac cgtcagcgaa cgcgacttcg agcaagaaat catgatgagc gagctccccg
     1501 tactggtgga gttttcggcg gagtggtgtg gcccttgcaa agtcgtcgct ccggagctcg
     1561 aggccctttc acgagagctc gacggcaaag cgaagatcgt caagatcgac atcgatcgct
     1621 cgccgatgat cgcgcgccag ctgggggttc aatcggtccc cacgttcgtc gtgtttcaaa
     1681 atgggcggcc cgtgggcggc aaggtcggcg cgctgaagcg caatcagctt cgcgagatgc
     1741 tcgatccatt tttgccccgc gcagaaggtg cgctgaagcc cgaagagctc gcggcgctgc
     1801 tcaaggccgg ccgcgtctcc gtcgtcgatg ctcgcgaacc tgcggtgtac gggcgcgccc
     1861 acatccaggg cgccgtgaat atccccggca gcgagatcga aggccgcctc gccgagcttc
     1921 acatgctgcc gatgccgccg gtcatctact gccgctccgg cgatctgacc aaagagctcg
     1981 ccgcgaagct cgcggagcag ggcgtccccg tcgcgttcct cgaaggcggc gtgctcggtt
     2041 gggaggcctc cggcttcacg ctgcaccggc ccgactgact tccgccgttt cccgagcggc
     2101 aggcttcagc tctttttttc gagcgcagcc agggccgccg cgatcacttc acgtaccggt
     2161 acgcccgcgg cgcgagcgag gttggcgcac acatcgaact cgggcttgcg ctgcggaggc
     2221 ccatagtccc cttcgctcac cttgacgggc acgcgaccgt agcgcgtctc gatctcgatc
     2281 acgcggcgcg gccgctcgtc gcgcgaaacc tgcgtccgtc gcactccgat gctgctcgtc
     2341 tctcgcagca tcgccgcggc gagagcgtct gcgcgatcgg acgccgcgag caccgagagc
     2401 gtcaaccccg ggcgcccctt cttcatcgtg attggctgag cccacgcgtc gagcgcgccc
     2461 tcgctgagca gcaccgaaat ggcgtgggcg gcgagctctc cggtcatgtc gtcgatgttc
     2521 gcctcgagca cgacgtggct cgcggcttca ctcgcggcga cggcgaacgg cgcaccgagc
     2581 acggcgcgca acgcgttcgg tcgatccggc agcttgcgag taccgcagcc aaagcccgtg
     2641 cgctcgggga cgaagcgcgg ccagctcaaa aacgcttcgg ccacggtggc gacgatggct
     2701 gctcccgtcg gcgtcacgag ctccccttcg attccggcgt cgtacgtggg gacaccgctc
     2761 aagcacaaga gcgtcgccgg cgcggggagc ggcaggcggc catgttggca gcgtacgaac
     2821 ccacggccca tgggcaacgg tgtcgcgaga acgcgagcac ccaagtactc gaagcacgct
     2881 gcggccccca cgatatccac gatggcgtct accgacccaa cctcgtgaaa actcacgctc
     2941 tcgagcggga cgcggtgcac ctccgcctcc gccttcgcga gccgcgcgaa tatccgcctc
     3001 gcgagctctg cggtcccttg cggcaagtcc gactcggcga tgagcgcgtc gatttgcgcg
//
LOCUS       DASTKS010000840         7057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1250248, whole genome shotgun sequence.
ACCESSION   DASTKS010000840 DASTKS010000000
VERSION     DASTKS010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250248"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFQ35_17325"
     CDS             complement(<1..541)
                     /locus_tag="VFQ35_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932469.1"
                     /translation="MTTETSAEAPRRVFRSVPPAVSSFDACIGLLSKVEEAPHDATGA
                     LTFEDGLVLVERRRVCWAISSSMGLYLTDLLCEGESRNIERCEIEQVYRRCKKRGLRL
                     SEELLASGLISQSRLQHVLREHNSEAIVRLSRSRGAPRFRRMARGGYDERFLLTPVEL
                     LAAIGARSGESRAQRAALEL"
     gene            complement(538..882)
                     /locus_tag="VFQ35_17330"
     CDS             complement(538..882)
                     /locus_tag="VFQ35_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932470.1"
                     /translation="MRALVALACLRTGIVLDLFGANGQEVEAGQFAAILPELFGASPS
                     ADWAAIFERLGAEDGPGFQDIVLLGDDAVHVVQRLVAPAGTALIGVASGTNNVGLVVS
                     LVRKKAAELEAR"
     gene            complement(879..1535)
                     /locus_tag="VFQ35_17335"
     CDS             complement(879..1535)
                     /locus_tag="VFQ35_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932471.1"
                     /translation="MMRVLMLDTDARLIRAVRRALEQKGLEVRAVEHLERARELTRRE
                     CFALVLADSNLISERDLSLFAGIPVVIVSAFLERAPAERLARRARMLQKPFTSAELCQ
                     VLDEEVGLPPHDDSLLDVLSRAHLSKSSFALSVGAGLLVLEDGELVHATVAEKRGEQA
                     LIEILLRGGKVERGAPSASSRTISRPFRPVMLDALCALERLDEVCRQPEPSRTRGGQP
                     "
     gene            complement(1532..2575)
                     /locus_tag="VFQ35_17340"
     CDS             complement(1532..2575)
                     /locus_tag="VFQ35_17340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932472.1"
                     /translation="MPTIDSSTNEMLALSAIDAPIAVCSTSGQLEVATPSALSLLRRL
                     GVVDRTPAPLPEDLWRLLERADVGEAVEWRPPHAKRDLLGCTRYAVSSRGSLLLMQEV
                     SEKHLAFSKRLHRQRLESTGRLVASIAHDIRSSVASIVYSADFLSVSGSSLTPEALKD
                     TVQEISEASRRLRLTVDGLLDYARLGPSIAIPVSLRDVLNRSQGLLRALYREGAHRLQ
                     VELSPGAEWASGNPISIEQIFVNLLLNAAEASSGPRVVSVSSVVSVAPDAPGGDPTHV
                     CVRVRDDGPGVPESVRDSIFDPFFTTKPNGTGIGLTAAREAAESLDGRLLLEPAEKGA
                     CFAVYLRRSEGPK"
     gene            2949..3314
                     /locus_tag="VFQ35_17345"
     CDS             2949..3314
                     /locus_tag="VFQ35_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932473.1"
                     /translation="MSNIKESLAKVMQLDGAMGACIVDSNSGMMLGAEGASSAINLEV
                     AAAGNTEVVRAKRRTMKNLNLKDTIEDILITLHTQYHIIRPLASNDALFIYLVLDKTR
                     GNLAMSRHQLAGIDRELTI"
     gene            3360..3728
                     /locus_tag="VFQ35_17350"
     CDS             3360..3728
                     /locus_tag="VFQ35_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932474.1"
                     /translation="MATEKQLTDVLEKISQDANGFIAASLVDLESGMTLAVKTSRTEF
                     DLAAASAYNSELVKQKLKIARTLGLSGSVEDMLITLTDQIHLVKMIGTTMFLYLAVDR
                     KQSNLGIVRTAVNKHVTAIT"
     gene            3749..5242
                     /locus_tag="VFQ35_17355"
     CDS             3749..5242
                     /locus_tag="VFQ35_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932475.1"
                     /translation="MPTIDDSRGVLVVRIVYDGPAMSGKTTSLRALSQGFAASVTSPE
                     ERDGRTLFFDWMDYVGGLFEGRPIRCQIVTVPGQTELAERRRRLLETADAVVVVADSR
                     RGEFGFGLGWVRETLEFLRTRQPPVGLVLQANKRDAADAVPREEMRLELNRIAPIALV
                     ESVATNKDGTREAFVLAVRLALDRVRTLAAKGQLVSGRPDEDDASELLASLQRKEREA
                     PKTERELDFRQAQSEDGPQSELAHLKRKPSEPRLSAVQHDETVFEPDPMMPGGMIWPP
                     VEGRTLLHEVSTLQIRPTRTRRGDWWGSASGWRVHSERDAIYGNVDAARHELIEWARL
                     HAATSAQLSPGRAVILADAGNGRLRLWQLVRVESALRERLATTLAQPEAAAVSHGLAD
                     VASRLVTARTWFHEGGVSLPCTLWTIGDQARYRPVYVGLMPRGGAAAVPEPAPLGLLE
                     REMMPHLRELRRTRVDFGEIQQRLVALGQDAPAESALAWLAALTRAI"
     gene            complement(5249..6607)
                     /locus_tag="VFQ35_17360"
     CDS             complement(5249..6607)
                     /locus_tag="VFQ35_17360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9932476.1"
                     /translation="MSETLKMLIVDEDQNALGSLSRDLSALGWEVRVASEPVRALQLA
                     EKERADAIVTELRAGAAGHTLVEGLRRRSAESCILVVSSALDVGTAVDVIRAGADDVA
                     EKPLRPSFIDARLREIMARRRSSATCAAREDAASAVMGETPSIRAVREQIRTVARFKD
                     LPVMITGETGTGKELVAKAIHELSEGPGPFVPINCSAIPEALLESELFGHEAGSFTGA
                     RGAHTGLFEAAGTGTLMLDELGEMPASLQPKLLRAIESRSFRRIGGSRDIPLRARIVS
                     ATHRRIIGKDSMLRSDLYYRLAGFTIATPALRQRVEDIDLLARHFLSRFCAHYRVELT
                     ISARALEALHTYHWPGNVRELRAVVEQAAVLARDGRVGVAEIVAGLRDRQELAPEDEP
                     EPGASGTLRKVVPGEPLRELERRTIKETWDSSGRNMSAAARALGLPRTTLRDRIRKYG
                     WR"
BASE COUNT         1242 a         2347 c         2300 g         1168 t
ORIGIN      
        1 ccaactcgag agctgccctc tgcgcgcgcg actctccgct gcgagctcca atcgccgcga
       61 ggagctcgac cggggtgagc agaaagcgtt cgtcgtaccc gccgcgagcc atgcgacgga
      121 agcgcggcgc cccgcgtgag cgcgagagac gcacgatggc ttcgctgttg tgctcgcgca
      181 gcacgtgttg caagcggctc tggctgatga gaccgctcgc gagcagctct tcgctgaggc
      241 gcagcccgcg cttcttgcag cggcggtaga cttgctcgat ctcgcagcgt tcgatgttgc
      301 gcgactcgcc ttcgcaaagc aggtccgtca agtagagacc catgctcgac gaaatcgccc
      361 agcacacccg tcgccgctcc acgagcacga gcccgtcttc gaaggtgagc gcaccggtgg
      421 catcgtgcgg cgcttcttcg actttcgaga gcaggccgat gcaggcgtcg aacgacgaga
      481 cagcgggcgg tacgctgcga aacacacgcc gaggcgcctc cgcgctcgtc tcggtcgtca
      541 ccgtgcctcg agctccgcag ccttctttcg caccagcgag acgaccagcc ccacgttgtt
      601 ggtccccgaa gcgacaccga tgagcgcggt tccggcggga gcgactagtc gctgcaccac
      661 gtgcacggcg tcgtcgccca ggagcacgat gtcttgaaat cctgggccgt cctccgcgcc
      721 gagccgctcg aagatcgcgg cccagtccgc gctgggcgac gcgccgaaaa gctcgggcaa
      781 aatcgcggcg aactgtccgg cctcgacttc ttgcccgttc gcgccgaaga ggtcgaggac
      841 gattcccgtg cgcaggcacg cgagcgccac gagcgctctc atggctggcc tccgcgggta
      901 cgactcggtt cgggctggcg gcacacttcg tcgagtcgct cgagcgcgca gagcgcgtcg
      961 agcatgacag ggcgaaacgg ccgcgatatc gtgcgcgagc tcgcggacgg cgcgccgcgc
     1021 tcgactttgc cgccgcgaag caggatctct atcagcgctt gctcgccacg cttttcggcg
     1081 acggtcgcgt gcacgagctc gccgtcctcg agcaccaaga gccctgcgcc cacggagagt
     1141 gcgaacgagc tcttgctcag gtgggcgcgg ctgaggacgt cgagcaggga gtcgtcgtgg
     1201 ggcggcaggc ccacttcttc gtccagtact tggcacagct cggcgctggt gaaaggcttc
     1261 tggagcatgc gcgcgcggcg agccaagcgc tctgccggag cgcgctccag gaaagccgag
     1321 acgatcacga cggggatgcc ggcgaagagc gagagatctc gctcgctgat gaggttgctg
     1381 tccgcgagca cgagcgcgaa gcactcgcgt cgagtcagtt cacgggcgcg ctcgagatgc
     1441 tcgacggcgc ggacctcgag ccccttctgt tccaaggcgc ggcgcaccgc tcgaatcagg
     1501 cgcgcgtcgg tgtcgagcat caagacgcgc atcatttcgg cccctcggag cggcgcaaat
     1561 acaccgcgaa acaggcgccc ttctcggcag gctcgagcag gagccgacca tcgaggctct
     1621 ccgccgcttc tcgagcggcg gtgagaccaa tgccggtacc gttcggcttg gtggtgaaga
     1681 acgggtcgaa gatcgagtcg cgtaccgact ctggaactcc cgggccgtcg tcccggacgc
     1741 gtacgcacac gtgcgttgga tcgccgccgg gcgcgtccgg cgccaccgag accacggagc
     1801 tcaccgacac gacgcgcggc ccgctcgatg cttcggcggc gttgagcagc aggttgacga
     1861 agatctgttc gatgctgatc gggttgccgg aagcccactc ggcgccgggc gagagctcaa
     1921 cttgcaagcg atgggctcct tcgcgataca gcgcccgcag caagccttgc gaacgattca
     1981 acacgtctcg gagggacacg ggaatggcga tgctcgggcc gagccgcgcg tagtcgagca
     2041 gtccgtccac cgttagccgg agccgccgac tcgcctccga gatttcctgt accgtatcct
     2101 tcagggcctc tggagtgagg gagcttccgc tgacggacaa gaagtcggcg ctgtagacga
     2161 tgctcgccac cgagctgcgg atatcgtgcg cgatgctcgc cacgaggcgc cccgtcgact
     2221 cgaggcgctg ccgatgtagg cgcttggaga acgccagatg cttctcggag acctcctgca
     2281 tcaagagcag gctgccccgg ctggacaccg cgtagcgcgt acaaccgagc aagtcgcgct
     2341 tggcgtgcgg cgggcgccac tcgaccgctt caccgacgtc cgcacgttcg agcaggcgcc
     2401 acagatcttc gggtagcggc gcgggcgtgc gatccactac gcccagccgt cgcaacaacg
     2461 agagcgcgga cggggttgcc acctcgagct gaccactggt cgagcacacc gctatcggcg
     2521 cgtcgatcgc gctcaacgcg agcatctcgt tggtggacga atcgatggtg ggcaccgcgc
     2581 gttgacccaa gcaagacgcg agccattgaa gcctgcgagt tcacggcgcg tcttggttcg
     2641 agccggagcc cgctcgtggt ctctgcgaaa acgaacggcg cgcagggcat cactgccggt
     2701 cgaaccggcc gtgtccgtcg ctctttactc gcacctggcg aagccactgg cgagccctgg
     2761 cggcacgtcg ccagttggcg gcgaagtccc gccacgccaa cggcgtcgaa agcgcgtctg
     2821 cccgcgcgaa aacgagcatt cgccccgtgg cgcgagcctt gcctagggct gcccacgccg
     2881 aactcgagac accgcgaaag cgcggacacg cacgagcggc agcgaccagg ggtcacggag
     2941 aaacatgcat gtcgaacatt aaagagagct tggcgaaggt gatgcaactc gacggcgcga
     3001 tgggcgcctg catcgtggat agcaacagcg ggatgatgct cggcgcggaa ggggcctcga
     3061 gcgcgatcaa cctcgaagtc gcggccgccg gcaacaccga ggtggttcgc gccaaacgcc
     3121 gcacgatgaa gaacctgaac ctcaaggaca ccatcgagga catcctcatc acgttgcaca
     3181 cgcagtatca catcatccga ccgctcgcga gcaacgacgc gctcttcatc tacctcgttc
     3241 tcgacaagac gcgcggcaat ctcgcgatgt ctcggcacca actggccggc atcgaccgcg
     3301 aactcacgat ttgagcgact cgacgcgttt gtgactcaac cactctgact aggagcacca
     3361 tggctacgga aaagcaattg accgacgttc tcgaaaagat ttctcaagac gcgaacggct
     3421 tcatcgctgc gtcgctggtc gacctcgaat ccggcatgac attggcggtg aagacctcac
     3481 gcaccgagtt cgacctcgcg gctgcgagcg cctacaatag cgagctcgtg aagcagaagc
     3541 tcaagatcgc gcggacgttg ggcctctccg gttcggtcga ggacatgctg ataacgctca
     3601 cggaccaaat ccatctcgtg aagatgattg gcacgacgat gttcctctat ctggccgtcg
     3661 atcggaagca aagtaacctc ggcatcgtga gaaccgcggt caacaagcac gtcaccgcca
     3721 tcacctgagc tccactgggc gcggccgcat gccaacgatc gacgactcac gcggtgtgct
     3781 cgtcgtgcgg atcgtttatg acggccccgc gatgtccggc aagacgacga gcctgcgcgc
     3841 tctgtcccag ggttttgccg cgagcgtcac gtcgccggaa gaacgggatg gccgaacgct
     3901 cttcttcgac tggatggact atgtcggcgg tctcttcgaa gggcgtccaa tccgatgcca
     3961 aatcgtgacg gtgcccggcc aaaccgagct cgccgaaagg cggcgccgtt tgctcgaaac
     4021 ggccgacgcg gtcgtggtcg tcgccgacag tcgacgcggt gagttcggtt tcggactcgg
     4081 ctgggtgcgc gagactctcg agttcttgcg caccaggcaa ccacccgtgg gtctcgtgct
     4141 ccaagccaac aagcgagacg ccgcggacgc agtcccgcgc gaagagatgc ggctcgagtt
     4201 gaatcggatc gctccgatcg cgctcgtgga gtcggtcgcc accaacaagg acggtacgcg
     4261 cgaagcattc gtgttggcgg tgcgcctcgc cctcgaccgg gtgcgcacac tcgcggcaaa
     4321 agggcagctc gtgagcggtc gccccgacga ggacgacgcg agcgagctcc tcgccagctt
     4381 gcaacgaaag gagcgagaag ccccaaagac ggagcgggag ctcgacttcc ggcaagcgca
     4441 gtcggaagat ggtccgcagt ccgagctcgc tcatctcaaa cgcaagccga gcgagccgcg
     4501 cctcagcgcc gtccagcacg acgagaccgt gttcgagccc gatccgatga tgcccggcgg
     4561 gatgatctgg ccgcccgtcg agggccgcac cttgctacac gaggtatcga cgctgcagat
     4621 tcgcccaacc cgcacgaggc gcggcgactg gtggggatcg gcgtccggct ggcgcgttca
     4681 ttcggagcgc gacgctatct acggtaacgt cgatgccgcc cgccacgagc tcatcgagtg
     4741 ggcgcggctg catgcggcta cgagcgcgca gttgtcgccg ggccgcgccg tgatcctcgc
     4801 cgacgccggc aacggtcggc ttcggttgtg gcagctcgtg cgcgtcgaat cggcgttgcg
     4861 cgagcggctc gcgacgacgc tcgcgcagcc tgaggccgcc gccgtttctc acggactcgc
     4921 ggatgtcgcg agtcgcctcg tcacggcgcg gacgtggttt catgaaggtg gggtctcttt
     4981 gccttgcacc ctctggacca tcggggacca agcgcgctat cgccctgtat atgtggggtt
     5041 gatgccgcgc ggtggagccg cagccgtgcc ggagccagcg ccgctcggct tgctcgaacg
     5101 agagatgatg ccgcacctcc gcgagctgag gcgcactcgc gtcgactttg gagagattca
     5161 gcagcggctc gtcgcgttgg ggcaagacgc gcccgcagag tcggctttgg cttggctcgc
     5221 ggctctcacg cgcgccattt gagagccgtc agcgccagcc gtactttcga atgcggtcgc
     5281 gcaaagtagt gcggggcagg cccagggcgc gggctgcggc gctcatgttg cggccgctcg
     5341 agtcccacgt ttccttgatg gtgcgacgtt cgagctcgcg cagtggctcg cccggtacga
     5401 cctttctcaa cgtacccgaa gcgcctggtt ctggctcgtc ctcgggagcg agttcctggc
     5461 gatcgcgcag cccggcgacg atctcggcga cgccgacgcg cccgtcgcgc gccaacacgg
     5521 ccgcctgctc cacgaccgcg cgcagctcgc gcacgttgcc gggccagtga taggtgtgga
     5581 gtgcctcgag agctcgagct gaaatcgtga gctcgactcg gtaatgtgcg cagaagcgcg
     5641 aaaggaagtg gcgcgccaac aggtcgatgt cctccacgcg ctgacggagc gcgggcgtcg
     5701 cgatggtgaa gccggccaag cgatagtaca ggtcggagcg cagcatcgag tctttcccga
     5761 tgatgcggcg gtgggttgcg ctcacgatcc gtgcgcgcag cggtatgtcg cggcttccgc
     5821 cgatgcgacg aaacgagcgc gattcgatcg cgcgcagcag ctttggttgg agtgaggcag
     5881 gcatctcgcc gagctcgtcg agcatcagcg tccccgtgcc tgcggcctcg aacagcccgg
     5941 tgtgcgcgcc gcgagctccg gtaaaagatc ccgcctcgtg accgaagagc tcgctctcga
     6001 ggagcgcctc cggaatcgcc gagcaattga tcggtacgaa cgggcccggt ccttcgctga
     6061 gctcgtgaat cgccttcgcg acgagctcct taccggtccc ggtctcgccc gtgatcatca
     6121 ccggcagatc cttgaatcgg gccacggtgc gaatttgttc gcgcaccgcg cggatgctcg
     6181 gcgtctcacc catcacggcc gatgccgcgt cctcgcgcgc cgcgcacgtc gcgctggagc
     6241 gccgcctcgc catgatctct cgcagccgcg cgtcgatgaa ggacgggcgc agcggctttt
     6301 cggcgacgtc gtcggcaccg gcgcgaatca cgtcgacagc ggtccccacg tcgagagcgc
     6361 tcgaaacgac caggatgcac gactccgcac tacgccggcg caagccttcc accaacgtgt
     6421 gtccggcggc acccgcgcgc agctccgtaa caatcgcgtc cgcgcgctcc ttctcggcga
     6481 gctgcagcgc tcgcaccggc tcggaggcaa cgcgcacctc ccatccgagc gctgacaagt
     6541 cacgagacaa agacccgagc gcgttctggt cttcgtccac gatcagcatc ttgagcgttt
     6601 cgctcatttc ggtccctgca gcgcgcggag cttggagttg ctcggcatcg gcgtgttcct
     6661 gaatgatccc ctgtggtttt tcgtcgactt ccgaacttgc acgttacgtg acgacagatc
     6721 tggtggcggg ccctcgctca gaacggcggt cgctgtcgaa acatcagcaa aatttgcgcg
     6781 cgcttccgcg ccgacgttgt gctcgaagcg accacgatgt tgacgcttct acgactgaat
     6841 ttccagcgcc acgaccgctc cgcgtcgcgc gtctcacgtt gagagcacgt ccggattcgt
     6901 cactcgcgcc gagcccatcg cgcgcgacga ctgcaatgta ctcggtcgtg cggatctggt
     6961 cagcggcgtc ctacgaacat cgacactcga tacatctcga agtgtacgag ttcgggctct
     7021 gcgctcaatt cacttcgcca aaacgtacga gttcggc
//
LOCUS       DASTKS010000841         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1474430, whole genome shotgun sequence.
ACCESSION   DASTKS010000841 DASTKS010000000
VERSION     DASTKS010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474430"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2719)
                     /locus_tag="VFQ35_17365"
     CDS             complement(<1..2719)
                     /locus_tag="VFQ35_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932477.1"
                     /translation="MKLVGSRVVRVFPRVARWLDGGARSRAAVAALLVACSGGESERL
                     NQAAGKSELALSSTADAQSKPKRDYTLFEAGAVRPIAVLPRGLVAVTNIPDDRVEFFR
                     AGARGLEPCGSVKVGMRPVAVASVGDRVWVVNHLSDSVSVVRVDAGSCRGVVERTLLV
                     GDEPRDVVVAPGARGELYGFVTTAHRGQNVTKPSGEARDPELSAPGVGRADVFVFRAD
                     RLGDDAGEADEKPLEVLTLFTDTPRALAVAPGKVFAAGFFSGNQTSVVRFQSVVQRGR
                     ESLAKLDRDGDLSIDADLPEAARVVEGGYPAIRGHGRCLSGTLASAPGRDRTDFRMDV
                     CVRTDPENPRRALEIVRQDGGVVTPSCSCTDATGEVQMTPPLIVRFYESASICGPNYV
                     ASLGGCWLEPPHDDADLAALEQNRQPPGMQAWNDSVAFRLPDRDVFTIDLNVSPPALV
                     PNGDFRHVGTTLFSMAVHPKSGKLYVGNTEARNHVRFEGPGALVPQDGRFANTTVRGH
                     VAESRISVLDPASRAVKPLHLNAHIDYSKCCEAGPNADTERSLAFPVGLAITSKRTRN
                     GQVADQQDLYVSALGSNKVAVLSTATLDRAQNGEMVQSPADHIEVPGGPVGLTLDESR
                     SRLYVLARFTNELVVIDTRSRRELERHRLYNPEPPSIVKGRPFLYDARNTSSHGDTAC
                     ASCHVFGDFDGLAWDLGAPDDRSFANLGPFFAKPEITSAPLTSRFLPVKGPMTTQSLR
                     GMANHGAMHWRGDRRGGLDSTVHVQPDTGAFDEQAAFKAFNVAFPGLNGRSAQLSPEQ
                     MQSFTDFILALSYPPNPIRNLDDSLTAAQKRARSRYFGCEITDESMARRECADGRNID
                     EETLGCNCLNPPEFVLGIEPRPAYCPKDPKCTLDVSDFQNTCNGCHTLDR"
BASE COUNT          496 a          898 c          900 g          472 t
ORIGIN      
        1 aacgatcgag cgtgtgacac ccgttgcacg tgttctgaaa gtcactcaca tcgagagtgc
       61 actttggatc cttggggcaa taagcggggc gcggctcgat accgagcacg aactcggggg
      121 ggttcaggca gttgcaaccg agcgtctctt cgtcgatgtt tcgaccgtcg gcgcattcac
      181 ggcgcgccat cgactcgtcc gtgatctcgc agccgaaata gcggctcctg gcacgctttt
      241 gagccgccgt gagcgagtca tccaagttgc ggatggggtt cggcgggtag ctgagcgcga
      301 gaatgaaatc ggtgaacgac tgcatctgct ctgggctgag ctgcgcagag cgcccgttca
      361 acccggggaa cgcgacgttg aaggccttga aagcggcctg ctcatcgaac gcgcccgtgt
      421 cgggctggac gtgaacggtg ctatcgaggc caccgcgccg gtcgcctcgc cagtgcatgg
      481 cgccatggtt cgccatgccg cgcagtgact gcgtggtcat cggacccttg acggggagga
      541 aacgcgaagt gagcggggcg ctcgtgatct ccggtttggc gaagaacggc cccagattgg
      601 cgaaggagcg gtcatcgggc gcgccgaggt cccaggcaag gccatcgaaa tcgccgaaca
      661 cgtggcaact tgcgcacgcg gtatcgccgt ggctcgaggt gttgcgtgcg tcgtagagga
      721 acggccgccc tttgacgatg ctgggcggct ccgggttgta caagcgatgc cgctcgagct
      781 ctcggcgcga tcgcgtgtcg attacgacga gctcgttcgt gaaccgcgcg aggacgtaga
      841 gtcgcgaccg cgattcgtcc aaagtgagcc ctacgggtcc acccggcacc tcgatgtgat
      901 ccgcgggaga ctgcaccatt tctccgtttt gggcgcggtc gagggtggcg gtgctcaaca
      961 cggctacctt gttcgagccg agggcgctca cgtacagatc ttgctgatcc gcgacttgac
     1021 cgttgcgagt tcgcttgctc gtgatggcga gccctacggg gaacgcgagg cttcgctcgg
     1081 tgtcggcgtt cgggcccgct tcacagcact tcgaatagtc gatgtgcgcg ttcaggtgca
     1141 gaggcttcac cgcgcgactc gcggggtcga ggacgctgat gcgactctcg gcgacgtgcc
     1201 ctcgcacggt cgtattggcg aatcgtccat cttgcgggac gagcgcgccg ggcccttcga
     1261 aacgcacgtg attgcgcgct tcggtgttgc ccacgtagag cttgccgctc ttcgggtgga
     1321 cggccatgct aaacaacgta gttccgacgt gacgaaagtc tccgttggga acgagcgcgg
     1381 gcggtgacac gttcaagtcg atggtgaaca cgtcgcgatc ggggaggcga aacgcgaccg
     1441 agtcgttcca cgcctgcatt cccgggggct gccgattctg ctcgagcgca gctaggtcgg
     1501 cgtcatcgtg gggaggctcg agccaacaac cgccgaggct cgcaacgtag ttgggaccgc
     1561 aaatggacgc cgattcgtaa aagcgaacga tgagcggtgg cgtcatttgc acctctcccg
     1621 tcgcgtccgt acacgagcag ctcggcgtga ccacgccgcc gtcttggcgt actatttcga
     1681 gcgcccgacg aggattctcc gggtccgttc gaacacaaac gtccatcctg aagtcggtgc
     1741 gatcacgccc cggcgcactt gcgagcgtgc ccgacaagca gcgtccgtga ccgcgaatcg
     1801 ccggatagcc accctcgacg acgcgggccg cctcgggcaa gtcggcgtcg atgctgagat
     1861 cgccgtcgcg atcgagcttc gccaggctct cccggccgcg ctgcacgacg ctttgaaagc
     1921 ggacgaccga ggtctggttg cccgagaaga acccggccgc aaacaccttt ccgggggcaa
     1981 cggcgagcgc tcgcggcgtg tcggtgaaca gcgtgagaac ctcgagtggc ttctcgtcgg
     2041 cttcgcccgc atcgtcaccc aggcgatcgg cgcgaaacac gaagacgtcg gcgcgaccca
     2101 cgccgggcgc cgacagctcc ggatcgcgcg cttctccgct cggcttcgtc acgttctgcc
     2161 cgcggtgcgc cgtggtcacg aagccgtaca gctcacctcg ggcgccagga gccacgacga
     2221 catcgcgcgg ctcgtcaccg accagcagcg ttcgctcgac gacgccccgg caacttcccg
     2281 cgtcgacccg gacgacactc acgctatcgg aaaggtgatt cacgacccag acgcgatcgc
     2341 ccacggaggc gacggcgacc ggacgcattc cgactttgac cgaaccgcac ggctcgagac
     2401 ctctcgcacc ggctcgaaag aactccacgc ggtcgtcggg gatgttcgtc accgcgacca
     2461 aaccgcgagg cagaactgca atcggccgca ccgcccccgc ttcgaagagc gtgtagtcgc
     2521 gctttggctt cgattgcgcg tcggccgtag acgagagggc gagctcggac ttccccgccg
     2581 cttggttgag cctctccgac tcgccaccgc tgcaagcaac gagcagcgcc gcgacagccg
     2641 cacgagagcg cgcgccgcca tcgagccaac gcgcaaccct aggaaatact cgaacgacac
     2701 gactgccaac gagcttcacc acagcgaccc ctttcgttga acgtgctcgg caccgacgaa
     2761 atcacc
//
LOCUS       DASTKS010000842         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1696977, whole genome shotgun sequence.
ACCESSION   DASTKS010000842 DASTKS010000000
VERSION     DASTKS010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696977"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFQ35_17370"
     CDS             <1..1006
                     /locus_tag="VFQ35_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HET9932478.1"
                     /translation="VDRILLAVSAAAEGDLTREVGVSGSDEIGRLGVGVQALITTLRS
                     NVASISRNAQALAATSEQMHAVGEQIQSSAEEGATRAQTAADSSVQISQSIQSLADSS
                     NLIANSIQEIATSSGKAADIAKAAVAMMERTSASIGELRERSRQIGNAVESISGIAQQ
                     TNMLALNAAIEAAHAGDVGRGFAVVANEVKQLSGETSRATAKITQEVRSIQASTQAVS
                     DCMHEIGEIVTEIDELQASIALSVKRQATTTQEITRVTASVAEDGRGISESVSGLAES
                     ARGTSEGLAEMQRAAEELASMATELQTFVDRFRFDTSDRAGSSAKSRRKGPAPVAEGP
                     "
     gene            complement(1014..1943)
                     /locus_tag="VFQ35_17375"
     CDS             complement(1014..1943)
                     /locus_tag="VFQ35_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932479.1"
                     /translation="MENRDFMALRASKLGVMCPIAGQLPPKRRLHTLGACLAVSLLAL
                     ACGSKEESKTLPSVQVAMDRTLAPVFEDGELELYEVRIGKQLPILAPSASQAEALAAQ
                     PMEPYAHAPWARVGQVRVQLTWTLTNLDDEPHNVEVLVDPWNEFAKYFPGLQLTDAER
                     GTYLPNLSAIDYLYAMDAVGGPDSRRHGTFTFDDCDEMARDFATALSLKANPPASDDP
                     EEDPTLRYVNHAFAFQNHSDNDPLVQKWVPPVAPALTGFDIGLRTFEPAKVAIEVVAE
                     VVDGGDDRVKTQGKSGTLLEAPTEVVTFGTTAP"
     gene            complement(2045..3439)
                     /locus_tag="VFQ35_17380"
     CDS             complement(2045..3439)
                     /locus_tag="VFQ35_17380"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9932480.1"
                     /translation="MTRSVQNPIVLRTAASWGTTVLATQLLASGQSLLLGEGEGAVVA
                     KPDGRDIADSPIRAVNNGWELDARGATGGEVHLRGRAENPALLARSGAPIPIVAGDWG
                     LIQYGSFSVFFQFSDAAPAIGRRGRFSWPLFLSFIFSALAVGGGLALIWAITTPPMIP
                     KPLELTTDAELAVQFNMKPEDLTPEPQVGKEEDKGSGVKDPGAKDTKEQGGGKKIQGA
                     EGKLGRNAKAEKTEQTGEPRAGLGGMAEVLSSDVGEEVKKTLGTISSVADALGGLRSQ
                     NIVLGQGSGFALKGGEKGGGGVGPDGVPFGSGTLDTGWGPGKGGGFGSGSGGPGGRGL
                     GGNGRGGVGDGEGSGSGTGGERKLAGKDAPKPGQGLGPEQIRRVVMSRMGAFTACYEI
                     AAARDPSLKGGVTVNFSISPGGSVSSVSVSNSSLSNPRVEGCITRQFGRLQFPAADKP
                     TNAGFPFVFRPSKK"
     gene            complement(3436..>3749)
                     /locus_tag="VFQ35_17385"
     CDS             complement(3436..>3749)
                     /locus_tag="VFQ35_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932481.1"
                     /translation="AKEDAPAPVEPAVKAPTEPEAKLRAAGTSQTEVPASKASVKEKE
                     GPEGVKTYQFGAIEVEGRQKSPQLIYFLRRVRAEFDAGALGHRSFLGELKDTRNDTAF
                     R"
BASE COUNT          627 a         1278 c         1230 g          614 t
ORIGIN      
        1 ggtcgaccga attttgctcg cggtctcggc cgcggcggag ggcgacctca cccgcgaggt
       61 cggcgtgagt ggcagcgacg aaatcgggcg gctcggggtc ggagtccagg cgctcatcac
      121 gaccctgcgc tcgaacgtgg cgagcatctc gcgcaacgcg caggcgctcg cggccacttc
      181 cgagcagatg cacgcggtgg gggagcaaat ccagagctcg gccgaggaag gcgcgacccg
      241 cgcccagacc gccgccgact cgtcggtcca aatcagccag agcatccagt cgctcgcgga
      301 cagctcgaac ctcatcgcga acagcatcca ggagatcgcg acgagctccg gcaaagccgc
      361 cgacatcgcg aaggccgcgg tcgcgatgat ggagcgcacg agcgcgagca tcggcgagct
      421 ccgcgaacgg agccgtcaga tcggcaacgc cgtcgagtcc atctcgggca tcgcgcagca
      481 gacgaacatg ctcgcgctca acgccgcgat cgaggctgct cacgctgggg acgtcggcag
      541 aggcttcgcc gtcgtcgcca acgaagtgaa gcagctctcc ggcgagacat cgcgcgcgac
      601 cgcgaagatt acccaagagg ttcgctcgat tcaggccagc acccaagcgg tgagcgactg
      661 catgcacgag atcggcgaga tcgtgaccga gatcgacgag ctgcaagcga gcatcgcgct
      721 ttcggtgaaa cgccaggcca ccacgaccca agagatcacg cgcgtgacgg cgtcggtcgc
      781 ggaggacggg cgcggtatct ccgagagtgt gtcggggctc gcagagtcgg cccgtggcac
      841 gtccgagggc ctcgccgaga tgcagcgcgc ggccgaggag cttgcgagca tggcgaccga
      901 gctgcaaacc ttcgtcgatc gttttcgctt cgacacgagc gatcgcgccg gctcgagcgc
      961 aaagtcgcgc cgcaaaggtc ccgcgcccgt cgccgagggc ccttagcctc cgctcacggg
     1021 gccgtggttc cgaacgtcac cacttcggtc ggcgcctcga gcaacgtgcc gcttttgccc
     1081 tgcgtcttca cgcggtcgtc cccaccgtcg acgacctccg cgacgacttc gatcgcgacc
     1141 ttggcaggtt cgaaagtgcg gaggccaatg tcgaacccgg tgagggcagg cgcgacggga
     1201 ggcacccatt tctgcaccag ggggtcgttg tccgaatggt tctggaacgc gaacgcgtgg
     1261 ttcacgtagc gcaaggtcgg atcctcctcg gggtcgtcgg acgcgggcgg gttcgccttg
     1321 agcgagagcg cggtcgcgaa atcgcgagcc atctcgtcgc agtcgtcgaa ggtgaaggtg
     1381 ccgtggcggc gcgaatccgg cccgcctacg gcgtccatcg cgtagaggta atcgatggcg
     1441 ctcagattcg gcaggtaggt gccgcgctcg gcgtcagtga gctgcaggcc gggaaagtac
     1501 ttcgcgaact cgttccacgg atcgaccaag acctcgacgt tgtgcggctc gtcgtcgagg
     1561 ttcgtcagcg tccaggtgag ctgcacgcgg acctgaccga cgcgagccca aggcgcgtgc
     1621 gcgtagggtt ccatcggttg cgcggcgagc gcttcggcct gcgacgcgct cggggcgagg
     1681 atgggcagct gcttgccgat gcgcacctcg tagagctcga gttcgccgtc ctcgaagacc
     1741 ggcgcgagcg tgcgatccat cgcgacctgc acgctcggca gcgtcttgct ctcttctttg
     1801 cttccgcacg cgagcgcgag cagcgaaacg gccaggcaag cacccaatgt gtgcaagcgt
     1861 cgcttggggg gaagctgccc cgcgatgggg cacatgaccc ccagtttcga agcgcgaagc
     1921 gccatgaaat ccctgttttc catgagcgga gccacgatca cttgcgatgc tcgtgccagg
     1981 ctcgactccg gagtcaccgg gttgatctgg cgagtgccgc gtcaccgcga ccacccgagc
     2041 tcgatcactt cttactggga cgaaagacga aggggaagcc ggcgttggtc ggcttgtcgg
     2101 cggccgggaa ttgcagtcgg ccaaactgcc gagtgatgca gccctcgacg cgcggattgg
     2161 agagcgacga gttcgaaaca ctcacgctgc tcaccgaacc cccggggctg atggagaagt
     2221 tcacggtgac tccgcccttc aggctcgggt cgcgcgcggc cgcgatttcg taacacgcgg
     2281 tgaacgcgcc catgcgcgac atcacgacgc gacggatctg ctcggggccg agtccctgac
     2341 caggctttgg tgcgtctttg ccagcgagtt tgcgctctcc gcccgtaccc gagccagagc
     2401 cctcgccgtc cccgacgccg ccacggccat tgccgcccaa gccgcgacct ccgggaccac
     2461 cgctaccgct accgaaaccc ccgccctttc cggggcccca gccggtatcg agcgtgccgg
     2521 agccgaacgg aacgccgtcg ggcccgactc cgccgccgcc cttctcgccg cctttcaggg
     2581 cgaaaccaga gccctgaccc aagacgatgt tctgcgagcg caagccgccg agcgcatcgg
     2641 cgacggagct aatcgtgccg agcgtcttct tcacctcttc gccgacgtca ctcgagagca
     2701 cttcggccat gcctccgagg cccgcgcgtg gctcgccggt ttgctcggtc ttctcggcct
     2761 tggcgttgcg cccgagcttg ccctcggcgc cctgaatctt cttcccgccg ccttgctctt
     2821 tcgtgtcctt ggcgcccgga tctttcacgc cggagccctt gtcttcttcc ttaccgactt
     2881 gcggctcggg agtgagatct tcgggcttca tgttgaactg caccgcgagc tccgcgtcgg
     2941 tcgtgagctc gagcggcttc ggaatcatcg gtggcgtcgt gatcgcccag atgagcgcga
     3001 gccctccgcc aaccgcgagc gccgagaaga tgaacgaaag gaacagcggc cagctgaaac
     3061 gaccgcgcct tccaatcgcc ggcgctgcgt cggagaattg gaagaaaacg gagaagctgc
     3121 cgtattggat gaggccccaa tcgccggcca cgatcggaat cggcgcgccg ctccgcgcga
     3181 gcagcgccgg gttctccgcg cgaccccgca aatgcacttc tccgcccgtc gcaccgcgcg
     3241 cgtcgagctc ccaaccgttg ttgacggcgc gaatcggcga gtcggcgatg tcgcgtccgt
     3301 ccggcttcgc aacgacggcg ccctcacctt ctccaaggag gagcgactgc cccgaagcga
     3361 gcagctgcgt cgcgagcacg gtggtgcccc aagacgccgc ggtgcgaagc acgatcggat
     3421 tctgaacgga tctcgtcatc ggaatgccgt gtcgttgcga gtgtccttga gctcacccaa
     3481 gaacgagcga tgcccgagcg cgccggcgtc gaactccgcg cgcacgcgac gcaagaagta
     3541 gatgagctgc ggcgacttct gccggccctc cacctcgatc gcgccgaact ggtaggtctt
     3601 caccccttcg ggcccctcct tctccttaac ggaggccttg ctcgcgggta cttcggtttg
     3661 gctcgtccca gctgcgcgca gctttgcttc cggctcggtc ggcgctttga cagccggctc
     3721 gacgggcgcg ggagcatctt ccttcgcgg
//
LOCUS       DASTKS010000843         9854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_803316, whole genome shotgun sequence.
ACCESSION   DASTKS010000843 DASTKS010000000
VERSION     DASTKS010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803316"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFQ35_17390"
     CDS             <1..705
                     /locus_tag="VFQ35_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9932482.1"
                     /translation="LVTLTSHGPFQYVPVAERSLVLGAIESTEFGGYLHSMRYEDEAV
                     ELLFERLEAAGLLANTTVVIYGDHDSRMRFPAFLAEGVELSKDILRLISERDYTTKQI
                     PLFIVLPPEKKRPAGTVSTLGGHVDVGPTLLYLYGLPRPRSFIGQSLLPARPGHALRV
                     DGSAVDEQRIYVATNGGRCESFPEFALRPLEECEALARAARSELDVSWSVTLSDLASE
                     LAGKRPAVSRAARSLH"
     gene            739..1683
                     /locus_tag="VFQ35_17395"
     CDS             739..1683
                     /locus_tag="VFQ35_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9932483.1"
                     /translation="MQKAFVIGGAGFIGSHLVDALSRRCGVTVYDNLSVGRRDFLEEA
                     LARGNTELVVGDVLDDEALSRAMQGHDVVFHLAANPEARRGLTNTRLDLEQGTVATQR
                     VLEAMKACGIRRVVLASSGTVYGDTSKACAESDLGALPISLYGASKLASEALVSAFVE
                     CFDFQGYVFRFGNVVGPRGTHGAMLDFFNKLKQNPAELEVLGDGRQSKPYVHVSDCVR
                     GMTFGLDNAHERSNVFNIAPPDCTTVAQIAELCVRASPYPNARLRFTGGERGWRGDVP
                     ISRLNPEKFAELGFRVSHSSDQAVEAAVIALAREVFAR"
     gene            1680..2384
                     /locus_tag="VFQ35_17400"
     CDS             1680..2384
                     /locus_tag="VFQ35_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET9932484.1"
                     /translation="MTAELAILAGGLATRLGDRTRTIPKSLLLVHGRPFVAWQLERVR
                     DSGFSKVLLCVGHLGEQIESFVGDGRAFGLHVEYSHDGGHLLGTGGALVKALPQLAEC
                     FVVTYGDSYLPFDYRAPLRDLLLHPEAEGCLAVYPNENRWDSSNTRVSGERVVAYRKN
                     ARDFDHIDYGALALRRSALAAEGRSAFGLDSVQTALAERGTLRAFIAGERFYEVGSDA
                     GIRDFEAYLSSHRPAY"
     gene            2822..4801
                     /locus_tag="VFQ35_17405"
     CDS             2822..4801
                     /locus_tag="VFQ35_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932485.1"
                     /translation="MKLASSFAYWSFLALVGACSAQSAGPERSSPNNNGSGSSSNSGY
                     GGSIGIIDVPVAGANTVGQPPMKKCDVCEDFPATPVFVNDAPMNAPSLFGEPSNVSQS
                     GGPCISEPQDGTLFPYNWLRPRIRWSGTSDVYEVRIKTARQKNDFVAYTTKTEFYIPR
                     DVWSGDKDGKSQGLAKNNFEEDITVTVRGVNKSGGTPVGSSVKFRTAAAEAAGTMVYW
                     ANVKAQTGDTVTEGDAAGWLMGFSVGDEDVVEALRVKDIKTQTRGYSANPKAVTCIGC
                     HTSTPDGEAVTFTSFFPWGGAIAGVTKTNRGQLPTGVTPSGLAGLQQQWLGAWTYTPA
                     EWRDGYKIAVTSFGPSMLGWPGGDLNKTNADNLLWVNIATPEPATAPTQDWELVQKWT
                     PEAQGKALGILPRNGDKRAALMPDFSNQPGNTTIAYTSSSTSQDGRIALINDTDIYTV
                     PFNRGAGGNATPLPGASAKGVAEYYPNYSGDDAFVAFNRIDNVMSLVKSNPVGFQNHL
                     YYRPESEIWITPVSGNNVSAANGETSAGSAVRLKANDPDTCGGKKSPGVLNSWAKFSP
                     TVTTVNGKTYYFLIFSSTRNTPDNQRAPSTNDMYKPVENLFSRLFMAAFTVEGGKVTT
                     YPAVYLWNQEENSNNLTPAWDNFKIPEVPPPVVPR"
     gene            4815..5810
                     /locus_tag="VFQ35_17410"
     CDS             4815..5810
                     /locus_tag="VFQ35_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET9932486.1"
                     /translation="MIVSRAPLRFSLGGGGSDLPAYYQRFGGFLVSAAIDRYVYVTAN
                     KRFYDDIRLSYSETEIVPRVADIRHRIFREALAMTHIERGIELTSVADLPANSGLGSS
                     GAFTVALLNALHTYNRDFVSSRLLAEEACELEMVRLGDPVGKQDQYIAAYGNITALEI
                     DTQGNVEVKPVPIRPEALDELEHNLLVAYTGIERPSRQVLSEQGQRLSQPESTAVDSM
                     HRIRELGHAVYGLLVRGDIDAYGELLHEHWCHKKKLASSVSDPTIDEYYEAIRRAGAL
                     GGKLMGAGGGGFFMFYVRPESRRRVCEELERLKLRQLRFRFELDGARIVANLHRA"
     gene            6029..7621
                     /locus_tag="VFQ35_17415"
     CDS             6029..7621
                     /locus_tag="VFQ35_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9932487.1"
                     /translation="MKPGSVLAGKYRLDSMLGSGGMGAVWRAEHLGLRAPVAIKLLAP
                     ELDGRQDALARFLREAQSAASLRSPHVVQVLDHGVDETTQTPFIAMELMEGESLADRL
                     TRVVRLSPAETSRIITQTARALNRAHELGIIHRDLKPANLFLVRNEDDEILKVLDFGL
                     AKWRRSPGALTQGAATATNIVMGTPYYMSPEQMNGSRDADQRSDLWALGVIAFECLSG
                     TRPFDGDNLIALGMKVCTAPTPALSHSLGLPPEFDAWFARALAKDPAHRFQTARELAE
                     ELRRISRVDGVASASWQPQPSAAPPLAAAPPAVVAHEVIAAGVQTAPLVQPPLEERPA
                     PTVPVNDGDATSENARSASPVARSIGTNSGPVRAPRRGRPLLWAGTAGIAVSTMAVIG
                     MVRSRPDRNAETAVQDRTSPRSVESSLPLGTASSLPPRAAEPSEAHPRSRDTEALTPP
                     EASATEVAPVVVPVPQASSGTAPPSAKSASPVSSNKPAVKPSISANEAIKVPPSAPTR
                     RPTQATSSSSAKGVDIERKPSF"
     gene            7752..8783
                     /locus_tag="VFQ35_17420"
     CDS             7752..8783
                     /locus_tag="VFQ35_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932488.1"
                     /translation="MMYQRERAHSSGVRLGAAVFMLSACLFASNAHAEDSAQVEARKH
                     FLQGVALVDQQRLAEALTEFEQCYALYPAFGTLYNIAQVHAALGHSVAAVETFEKYLA
                     EGDSSIPAKQRARAEAELAAQRQRIGELTVQVTPADAQLLIDDNVVARSGAAITVRLA
                     AGPHQVSAVLVGHRTEQRPVDIVAQNQVNLTLTLEPLPPANAPVLPPSTPPAPSPTEP
                     LPSSSSKQKIIGLAVGGAGLVGAGVGLGIALAGQAKHRDALDQWAAGDKASARETESA
                     SSRQKTAGYVVIGVGGAMALAGAIVAFTARPTASSSASAATAKARSDFSWSPWISTSL
                     LGASLQQRW"
     gene            8788..>9854
                     /locus_tag="VFQ35_17425"
     CDS             8788..>9854
                     /locus_tag="VFQ35_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932489.1"
                     /translation="MRKIRFSTGVAVAAIATLLLECSGGLPDDEGTGGAGRGGGGTGS
                     GGKSASLGGTRPAGGESGASAGTTEPMTEGGTTAATQGGTGGTLGSGGDSALGGITHM
                     GGAAGAGGAGGELAGAGGAVAAGGRHEPSGGSAGTAGKCGTRQCDSSADNDCSGKPDN
                     QDSTCGCPTADAVRDCGTGKSGICASGTQQCKFAQDRASSAWSACVGPLAGTRDCRSS
                     ADNNCNGVADNQETECTACVVGAPHACNTHPEDGKGICKAGTQTCALSSDNTSVAYGA
                     CSGDIGPTTETCGPFESDGKTPVPDANCNGLAGDGDYTQTGSKCATAVTVGGKAVHLF
                     DANRPGTVAIHNCVTACTVVSG"
BASE COUNT         1805 a         3243 c         3191 g         1615 t
ORIGIN      
        1 ctggtgacgc tcacctcaca cggccctttt cagtacgttc ccgtggccga gcgctcgctc
       61 gtgctcggcg cgatcgagag caccgagttc ggcgggtact tgcactcgat gcgctacgag
      121 gacgaagcgg tcgagctgct cttcgagcgc ctcgaggcgg ccggcttgct cgcgaatacg
      181 accgtcgtga tctacggaga tcacgactcg cgcatgcgct ttcccgcctt cctcgcggag
      241 ggagtggagc tctccaaaga cattttgcgg ctgatctcgg agcgcgacta cacgaccaag
      301 cagatcccgt tgttcatcgt tttgcccccg gaaaagaagc gccccgcggg cacggtaagc
      361 acgcttggtg gccatgtcga tgttggcccc accctcctct acctctatgg attgccccgg
      421 ccgcgttcct tcatcgggca aagcttgctt ccggcgagac ccgggcacgc gctccgcgtc
      481 gacggctcgg ccgtagacga gcagcgcatt tacgtcgcca caaacggcgg gcgctgcgaa
      541 agctttccgg agttcgcgct ccgccccctc gaagaatgcg aagcgctcgc gcgggcggct
      601 cgcagcgagc tcgacgtgag ctggagcgtc acgctgtcgg atttggcgag cgagctagcc
      661 ggaaaacgtc cggccgtgag ccgcgccgcg cgctctctgc attgacgcgg cgcgcgcatc
      721 cgagaaacat gcgtcgacat gcagaaggcg ttcgttatcg gcggcgcggg cttcattggg
      781 agtcacttgg tcgacgcgct ttcgcggcgc tgcggcgtga ccgtctacga caacctctcc
      841 gtgggccgcc gtgacttcct cgaagaggcc ctcgcgcggg gcaacaccga gctcgtcgtg
      901 ggggacgtgc tcgacgatga ggctctgtca cgcgccatgc agggacacga cgtcgtcttt
      961 cacctggcgg cgaaccccga ggctcggcgc ggtctcacga atacgcggct cgatttggaa
     1021 caaggcaccg tcgcgacgca gcgcgtcctc gaagcgatga aggcgtgcgg gattcgccgt
     1081 gtcgtcctcg cttcgtccgg taccgtgtac ggagacacga gcaaggcgtg cgcagagagc
     1141 gacctcggcg cgctcccgat ctctttgtac ggtgccagca agctcgcctc ggaagcgctc
     1201 gtaagcgcct tcgtcgagtg cttcgacttc cagggctatg tgttccgctt cggcaatgtc
     1261 gtcggaccgc ggggcacgca tggcgcgatg ctcgacttct tcaataagct gaagcaaaac
     1321 ccagccgagc tcgaggtgct cggtgacggt cgccagtcga agccgtacgt acacgtgagt
     1381 gactgtgtgc gcggcatgac gttcgggctc gacaatgctc acgagcgctc gaacgtcttc
     1441 aacatcgctc cccccgactg caccaccgtc gcgcaaatcg ccgagctttg cgtgcgcgcg
     1501 tcaccgtacc cgaacgcgcg gctccgcttc accggcggcg agcgcggctg gcgcggcgac
     1561 gtgccgatat cgcgcctcaa cccagagaaa ttcgccgagc tcgggtttcg cgtcagccac
     1621 tcgagcgatc aggcggtcga ggccgccgtc atcgcgctgg ctcgcgaggt gttcgcccgg
     1681 tgaccgcgga actcgccatc ttggcgggag ggctcgcgac gcgactcgga gatcgcacgc
     1741 gaacgattcc gaaatcgctt ttactagtgc acggtcgccc gttcgtcgcc tggcagctcg
     1801 agcgcgtccg cgactccggc ttctcgaagg tgctgctgtg cgtcggacat ttgggcgagc
     1861 aaatcgagag cttcgtcggc gatgggcgcg cgttcgggct gcacgtcgaa tactcgcatg
     1921 acggaggtca cctgctcggt acgggcgggg ccctggtgaa ggcgctcccg cagctcgcgg
     1981 agtgtttcgt cgtgacgtac ggcgacagct atcttccctt cgattatcga gcgccgcttc
     2041 gcgaccttct gctgcatccc gaggcggagg gctgtctcgc ggtgtatccc aacgaaaacc
     2101 gctgggattc gagcaatacg cgcgtcagcg gagagcgcgt cgtcgcctat cggaagaacg
     2161 cgcgggactt cgaccacatc gactacggag cgctcgcttt gcgtcgctcc gcgctcgctg
     2221 cggaaggccg gagcgcattc gggctcgata gcgttcagac ggcgctcgcc gagcgcggca
     2281 ccttgcgcgc cttcattgcg ggcgaacgct tttacgaggt cggctccgac gcaggaatcc
     2341 gggatttcga agcgtacttg agctcgcacc gccccgctta ttgaactctc gtccacggaa
     2401 gctcagcggg attttgtcac cgcggctgca tcctttgagc gaccctcgga accatctcga
     2461 aacgagtcgc cttttcgcgg gcaatgggcc ccgcgagctg ccccgatttg gcttcgtaga
     2521 cggctccgcc gcaaacgaaa gcatcgaaac gaacttcttc gcagcgcgag tgaaatcgac
     2581 tcgatcatgc cgaggttgcg gcaatcgaaa cgcgaggagg catcgcggca cgggcagatg
     2641 tgatgaatgt tcgaacaccg ctgcgatgac gtgccgcccg gcccgacggg gtcgaccgcc
     2701 tacacgcagc gccacgccgc tgggttgcgc ccgcagatct gccgtgtcag gaaacatccg
     2761 agcgcttcga aagccccacg cgagtgggac gagcgccctt tcttcttagt accgaggccg
     2821 aatgaaactc gcgagctcct tcgcttattg gtcatttctt gcgctggtcg gagcatgctc
     2881 cgcgcaaagc gcagggcctg agcgctcgtc accgaacaac aacgggagtg ggagttcttc
     2941 gaattcgggc tacgggggca gcatcggcat catcgacgta ccggtcgcag gcgccaacac
     3001 ggttgggcag cctccgatga agaagtgcga cgtgtgcgaa gatttccccg ccacacccgt
     3061 attcgtcaac gacgctccca tgaacgcgcc gagcctcttc ggcgaaccga gcaacgtgag
     3121 ccagagcggc ggaccgtgca tctcggagcc gcaagacgga acgctgtttc cctacaactg
     3181 gttgcggccg cgcattcgtt ggagcggcac gagcgacgtc tacgaagtgc gcatcaagac
     3241 ggcgcgccaa aagaacgact tcgtcgcgta cacgaccaag accgagtttt acatcccgcg
     3301 cgatgtttgg tccggcgata aagacggaaa gagccagggc ctcgccaaga acaatttcga
     3361 agaggacatc accgtcaccg tgcgcggcgt gaacaagagc ggcggcacac cggtcggttc
     3421 gagcgtgaag tttcggaccg cggcggccga ggccgcgggg acgatggtgt attgggcgaa
     3481 cgtcaaagcg caaaccggcg acaccgtcac cgagggcgac gcggcggggt ggctcatggg
     3541 cttttccgtg ggtgacgagg acgtcgtcga agcgctccgc gtcaaggaca tcaagacgca
     3601 gacccggggc tactccgcca atccgaaagc ggtgacttgc atcgggtgtc acacgtcgac
     3661 accagacggc gaggctgtca ctttcacgtc gtttttccct tggggcggag cgatcgcggg
     3721 cgtgaccaaa acgaaccgtg ggcagcttcc gactggggtc acgccgagcg gccttgcggg
     3781 tctccagcag cagtggctcg gggcgtggac ctacacgccc gcagaatggc gcgacggcta
     3841 caagatcgcc gtgacgagct tcggaccgag catgctcggt tggccaggcg gtgacctcaa
     3901 caagacgaac gccgacaatc tcttgtgggt aaacatcgcc actcccgagc cggcgaccgc
     3961 gccgacgcaa gattgggagc tcgtgcaaaa gtggacgccc gaggcgcagg gtaaggcgct
     4021 cggcatcttg ccgcgtaacg gcgacaagcg cgcggcgctc atgcccgact tttcgaacca
     4081 gccgggcaac accaccatcg cttacacctc gagctccacg agccaagacg gccgtatcgc
     4141 gctcatcaac gacaccgaca tctacacggt gccgttcaac aggggcgctg gcggcaacgc
     4201 gacgcctctg cccggtgctt ctgccaaggg cgttgccgag tactacccga actactcggg
     4261 cgacgacgcc ttcgtcgcgt tcaatcgcat cgacaacgtg atgagcttgg tgaagtcgaa
     4321 tccggtcggc ttccaaaacc acctctacta tcggccggaa agcgagatct ggatcacgcc
     4381 cgtttccgga aacaacgttt cggcggccaa tggagagacc agcgcgggca gcgccgttcg
     4441 cttgaaggcg aacgatccgg acacgtgcgg cggcaagaag agccccgggg tgctgaactc
     4501 gtgggcgaag ttctcgccga ccgtcaccac cgtgaacgga aagacgtact acttcctcat
     4561 cttctcttcg acccgcaaca cgccggacaa ccagcgcgcg ccgagcacga acgacatgta
     4621 caagccggtg gagaacctct tctctcggct gttcatggcg gcgtttacgg tcgaaggcgg
     4681 caaggtgacg acttaccccg ccgtgtacct ctggaaccag gaagagaact cgaacaacct
     4741 cacgccggct tgggacaact tcaagattcc ggaagttccg ccccccgtcg tccctcgctg
     4801 aaggcgttcg ctcgatgatc gtctcgcgcg cgccgcttcg tttttctctc ggcggcggcg
     4861 gcagcgatct gcctgcctat taccaaaggt tcggcgggtt cctggtgagc gccgccatcg
     4921 accgatacgt gtacgtcacc gccaacaagc gtttttacga cgacattcgg ctctcgtact
     4981 ccgagaccga gatcgtgccg cgcgtcgccg acatccggca tcgcatcttt cgtgaggcgc
     5041 tggcgatgac gcacatcgag cgcggcatcg agctcaccag cgtcgccgac ttgcccgcga
     5101 atagcggcct cggctcctcc ggagcgttca cggtggcgct gctcaatgcc ctccacacct
     5161 acaaccgcga cttcgtctct tcgcgcctct tggcagagga agcgtgcgag ctcgagatgg
     5221 tgcgcctcgg tgatccggtt gggaagcagg accagtacat cgctgcttac ggcaacatca
     5281 ccgcgctcga aatcgacacc cagggcaacg tcgaggtcaa accggtccca attcgcccgg
     5341 aggcgctcga cgagctcgag cacaacctgc tcgtcgcgta cacgggcatc gaacgaccat
     5401 cgcgccaggt actcagcgag cagggccagc gcctgagcca acccgagagc accgccgtcg
     5461 actcgatgca tcgcattcgg gagctcggcc acgccgtcta cggcctgcta gtgcgcggcg
     5521 acatcgacgc ctatggcgag ctcttgcacg aacactggtg ccacaagaaa aagctcgcat
     5581 cgagcgtcag cgacccgacc atcgacgaat actacgaggc gatacgccgc gccggggccc
     5641 tcggcggcaa gctgatgggc gcgggcggtg gaggcttctt catgttctac gtgcgccccg
     5701 aatcacggcg tcgcgtttgc gaagagctcg agcgcttgaa gctccgtcag ctccgcttcc
     5761 gcttcgagct cgacggcgcc cgcatcgtcg cgaacctgca ccgcgcataa cctgccacga
     5821 cgtctcgcaa ggttcggaac gacgcctcgc tgagtcacgg cccgccgcga gcatcggagg
     5881 cggtccccta catcgctaca gtgtgcgcat ttccgcgtga atggaatgat gggccgcggt
     5941 ggaacggact tcgcgcagcg agttctttgc tcggtcccaa ccagacgcta ggttagcggt
     6001 cccgaggcga agcccaatcg cgaacgacat gaaacctggt tccgtgctgg ccggcaaata
     6061 ccgactggac tcgatgcttg gcagcggcgg gatgggcgcg gtttggcgcg ccgagcatct
     6121 cgggcttcgc gcgccggttg cgatcaagtt gctcgcgcca gagctcgacg gtcgacaaga
     6181 cgcgctggcg cgctttctac gtgaagccca gtcggcggcg agcctgcgga gcccgcacgt
     6241 agtccaggtg ttggatcacg gggtcgacga aaccacccaa acgccgttca tcgccatgga
     6301 gctgatggag ggtgaaagcc tcgccgaccg attgacgcgc gtcgtgcggc tctcacccgc
     6361 cgagacgtcg cgcatcatca cccagaccgc gcgggcgttg aatcgtgcgc acgagctcgg
     6421 aatcattcac cgcgacctga agcctgcgaa tttgttcctg gttcgcaacg aggacgacga
     6481 gatcctaaag gtgctcgatt tcggtctggc gaaatggcgg cgctcgccgg gcgcgctcac
     6541 gcaaggagcg gccacggcga ccaacatcgt gatgggcacc ccctattaca tgagcccgga
     6601 gcagatgaac ggctctcggg acgccgacca gcgcagcgat ctgtgggcgc tcggcgtgat
     6661 cgcgttcgag tgcctgagtg gaacgcgccc cttcgatggc gacaacctga tcgccctcgg
     6721 catgaaggtc tgcaccgcgc cgacgccggc gctcagtcat tcactggggt tacccccgga
     6781 gttcgacgcc tggttcgcgc gcgctctggc gaaagatcca gcccaccgct ttcaaacggc
     6841 gcgtgagctc gccgaagaat tgcgaagaat cagcagggtg gatggtgtgg cgtccgcctc
     6901 gtggcagccc caaccgtcgg ctgcgccgcc gctcgccgcg gctcctcccg ccgtcgttgc
     6961 gcacgaagtc atcgcggcgg gcgttcaaac ggctcccctc gttcagccgc cgcttgagga
     7021 gcgccccgca ccgaccgtgc cggtgaacga cggcgacgcg acttccgaaa atgcgcgaag
     7081 cgcttcgcca gtcgcgcgca gcatcgggac aaactccggg cccgtgcgcg ctccgcgtcg
     7141 ggggcgacca ctgctttggg cgggaacggc gggcatcgcc gtctcgacca tggcggtcat
     7201 cggcatggtt cgaagtcgtc ccgaccgaaa cgccgaaacg gcggtgcaag atcgcacgtc
     7261 gccacgttcc gtcgagtcat cgctgccgct gggtacggcg tcgtcactgc cgccgagagc
     7321 agccgagcca tcggaggcgc atccgaggag ccgagacacg gaggcgctca cgccgccgga
     7381 agcgagcgcg acggaagtag cgcctgtcgt cgtccccgtc ccacaagcga gcagcggcac
     7441 cgcaccgccg tccgctaagt cggcgtcacc cgtgagctcg aacaagcccg cggtgaagcc
     7501 gagcatatct gcgaacgagg ccatcaaagt acccccgagc gcgcctactc gccggccgac
     7561 tcaggcgacc tcatcgagtt cagccaaggg cgtggacatc gagcgcaagc cgagtttctg
     7621 agtgcgtgag cgagctgcca tttgccagcg cgcctcgtcg aggggcgaag ccggttcttg
     7681 acagcccgga cgcggtgcgc gaccgtcgct tcgcgagaag tcgtcgacgc tgctcggagt
     7741 agtgactctg catgatgtac caacgtgagc gagcacattc atccggtgtg cggctcgggg
     7801 cagcagtatt catgctctct gcttgcttgt tcgcctcgaa cgcgcacgcg gaggattcgg
     7861 cgcaagtcga agcgcggaag cactttctgc agggcgttgc gttggtcgac cagcaacggc
     7921 tcgctgaagc gctcaccgag ttcgagcaat gttacgcgct ctatccggcg tttggcacgc
     7981 tgtacaacat cgcccaggtc cacgccgcgc tcgggcactc ggtcgcggcc gtcgagacgt
     8041 tcgagaagta cctggcggaa ggcgacagct ccataccagc caaacaacgc gctcgcgcag
     8101 aagccgagct cgccgctcag cgccagcgca tcggtgagct caccgtgcaa gtcacccccg
     8161 cggatgcgca gctcttgatc gacgacaacg tggttgctcg cagcggcgcc gccatcaccg
     8221 tccgcctcgc agcggggcca caccaggtga gcgccgttct cgtcgggcat cgcaccgaac
     8281 agcgcccggt cgacatcgtc gcgcagaacc aagtcaacct caccttgacg ctcgaaccgt
     8341 tgcctccagc gaacgcgccg gtgctaccgc catcgacacc ccccgcgccg tcgccaacgg
     8401 agccgcttcc gagcagctcc tcgaagcaaa agatcattgg tctcgccgtc ggtggcgccg
     8461 ggttggtggg agccggagtc gggctcggga ttgcgctcgc aggccaggcc aaacaccgcg
     8521 acgcgctcga ccaatgggcg gccggagaca aagcctccgc gcgcgaaacc gagtccgctt
     8581 cgtctcgcca gaaaacggcg gggtacgtcg tgatcggagt cggtggcgcg atggcgctgg
     8641 ctggtgcaat cgtggcgttc accgcgcgcc cgacggcatc gtcatctgcg tcagcagcca
     8701 ccgcgaaagc gcgctccgat ttcagctggt cgccgtggat ctcgacgtcg ctcttgggcg
     8761 cgagcctgca acagcgttgg taacgatatg agaaagatcc gcttctccac tggcgtcgca
     8821 gtcgctgcca tagcgacgct cctcctcgag tgttccggcg ggcttcccga cgacgaaggg
     8881 acgggcggcg ctggccgagg cggcggaggc accggaagcg gaggaaagtc cgcttccctc
     8941 ggcgggacgc gccccgccgg cggcgaatcc ggcgcaagcg cgggaaccac cgagccgatg
     9001 acggaaggcg gaaccaccgc tgcgacgcag gggggaacgg gcggtaccct cggctcgggg
     9061 ggagactcgg cgttgggcgg tatcacccac atgggtggag ccgcgggcgc gggcggagcg
     9121 gggggagagc tggctggcgc gggtggcgcc gtcgcagcgg gcggccgcca cgaaccaagc
     9181 gggggaagcg ccggcacggc gggcaagtgt gggacgcgcc aatgcgactc cagcgccgac
     9241 aacgactgca gcggaaagcc ggacaatcag gactcgacgt gcggatgtcc aaccgcggac
     9301 gcggttcgcg attgcggcac cggcaagtcg ggaatctgcg cgagcgggac acagcagtgc
     9361 aagttcgcgc aggacagggc ctcgtccgca tggagcgcct gcgtcgggcc gctcgccggg
     9421 acgcgagact gccgctcgtc cgccgacaac aactgcaacg gtgtcgccga caaccaagag
     9481 acggagtgca ccgcttgcgt ggtgggcgcg ccgcacgctt gcaacacgca ccccgaagac
     9541 ggaaagggca tttgcaaagc tggcacgcaa acgtgcgcgt tgtcttcgga caacacgtcc
     9601 gtcgcgtacg gcgcttgcag cggtgacatc gggccgacca ccgaaacttg cggcccattc
     9661 gaatcagacg gcaaaacgcc ggtgcccgac gcgaactgca acggactagc gggcgacggc
     9721 gattacactc agacggggtc gaagtgcgcg actgcggtga ccgttggcgg caaagccgtg
     9781 cacctcttcg acgccaaccg cccggggacg gtagccatcc acaattgcgt tacggcttgc
     9841 acggtagtga gtgg
//
LOCUS       DASTKS010000844         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1921703, whole genome shotgun sequence.
ACCESSION   DASTKS010000844 DASTKS010000000
VERSION     DASTKS010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921703"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ35_17430"
     CDS             complement(<1..451)
                     /locus_tag="VFQ35_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9932490.1"
                     /translation="MMAANKQRPDLQKSLVGFVVGDVVYAVGISSVREIVNPVPLTEL
                     PHAPPAVAGVMDHRGEVVPVVDLRARFGLPHNADPRRSKWILVDVGGRTVGLAVDRVT
                     DVFGTGGGDIRPAPTLGRGDDERGIVGVTKHEGVLVFVLDIVDFDLLT"
     gene            complement(451..933)
                     /locus_tag="VFQ35_17435"
     CDS             complement(451..933)
                     /locus_tag="VFQ35_17435"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9932491.1"
                     /translation="MADLARAAPARPLRRGTDRGPVREFLVFGLAGELYGVELTGIKE
                     ILSPPPITPVPRAPREVVGVCSVRGLLVTVLDLRRRLRLEERPLGRRSRILLATADAG
                     EVTGLLVDEVRQVVRLSENEIEVAAQALGGDLAEHVLGVGRPGGMFLILLDLNAIVKD
                     "
     gene            complement(926..3055)
                     /locus_tag="VFQ35_17440"
     CDS             complement(926..3055)
                     /locus_tag="VFQ35_17440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9932492.1"
                     /translation="MAEISDKAREEFFSEAQEIIETLSRNLLSLDSASKQGTPDPSLV
                     NEAFRAVHTLKGLAGLFGATRLGALSHRLEDLLDALRLGRMALAADTLDVLFSAVDAY
                     NKVLEQEKAGIDEPLPEADALLAKLERLGAHVPEQSSVVSEYEFEPGMLAVLTEYEEH
                     RLKVNIEQRLSLYRLRVQFDLATIDRALEQIKEKARLHGEIITYLPTGETTSADSIEL
                     DLLMASKDDLATLIGALGADNVVIEEIPRRRAQDAGPSRTSARASVLAMPMPSAPVRA
                     GDSVPPPVRESDRGGPMEKERSLRSLTQTVRVDIRKLDHLMNIVGELAIVRGALSRTA
                     ERVRNDGLPKLAAELMRLHRNFDRRLSEMQDGILEVRMVPLGQVFERLARVVRQISRE
                     LHKEIRLVITGAETEIDKLIVEELSDPLMHMIRNAIDHGIESSDKRAQVGKPAAGTIA
                     LNAYQKGNHVMIEVEDDGRGIDESAIVERAVQLGKVERTEVGELAREEILGLIFLPGL
                     STRQSADDVSGRGVGMDIVKTNIGKLGGVIDVHSEPGIGTKMTITLPITLAIVSALIV
                     RAEAQVFALPLASVSEALSLDESSVRVLDGREVVTLRGSTLPICRLSRLLNLDRAPEA
                     PARQGFVVVIALGSRRLGLVVDHLYGQQDIVIKPLGRSLRGVRGFAGATELGDQRVGL
                     VLDAAALIEEVLVGTEPQRALSRSLHG"
     gene            complement(3048..3455)
                     /locus_tag="VFQ35_17445"
     CDS             complement(3048..3455)
                     /locus_tag="VFQ35_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932493.1"
                     /translation="MPRILVVEDSPTMRAFIRAALEDQVSSAGELSVVEASSGFDALR
                     LLPRGPYALVITDINMPDINGLELVRFIRGSEQHQKTPILLISTQSSERDQQRGLALG
                     ADAYLPKPFTEELLRKAALEQLERPRVKASPHG"
     gene            3870..>4439
                     /locus_tag="VFQ35_17450"
     CDS             3870..>4439
                     /locus_tag="VFQ35_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9932494.1"
                     /translation="MIGAGASGLVSCKALEDAGVAYECFEASDRVGGLWVFKNPNGKS
                     AAYRSLHINTSRERMQFRDYPMPRDYPDYPEHRLIARYLESYADHFGLASRIRFNTTV
                     ERVAPRPRGGFSVTLHGAETQVFDAVVVANGHHWDPRWPEPRIPGSFDGLELHSHQYV
                     DPTEPYALAGKRVAVVGIGNSAVDIASELA"
BASE COUNT          763 a         1484 c         1435 g          757 t
ORIGIN      
        1 gagttagaag gtcgaagtcc acgatgtcga ggacgaacac caaaacgccc tcgtgcttgg
       61 tgacgccgac gatgccgcgc tcatcgtcgc ctcgcccgag cgtcggcgcg ggccggatgt
      121 cacctccacc ggtgccgaac acgtccgtga cgcggtcgac ggccaagccc accgtgcgtc
      181 cgccgacgtc gaccagaatc catttggacc ggcgcgggtc ggcgttgtgc ggcaagccga
      241 agcgagcgcg cagatcgacg accggcacga cctcgccgcg gtggtccatc acccccgcca
      301 ccgccggagg cgcgtgcgga agctccgtga gcggcaccgg gttcacgatc tcgcgcaccg
      361 aagagatgcc caccgcatag acgacatcgc ccacgacgaa gccaaccagg ctcttttgca
      421 aatcagggcg ctgcttgttg gccgccatca tcaatccttc acgatcgcgt tgaggtccaa
      481 gaggatgagg aacatccctc ccgggcgccc gacgccgagc acgtgctcgg cgagatctcc
      541 acccagcgcc tgagctgcga cctcgatctc gttttcggag aggcggacga cttgccggac
      601 ctcgtcgacc aagagccccg tcacctcgcc cgcgtcggcg gtcgccaaca gaatgcgcga
      661 acgacggccg agcggacgct cctcgaggcg cagccgccgc ctcaggtcga gcacggtcac
      721 gagcaagccg cgcacgctgc acaccccgac cacttcgcgc ggagcacgcg gcaccggggt
      781 gatggggggc gggctcaaga tctctttgat gcccgtgagc tcgacgccgt agagctcgcc
      841 cgcgagcccg aagaccagga actcgcggac aggaccgcgg tcggtgccgc gccgcaaggg
      901 acgcgccggt gccgctcgcg ccaggtcagc catgcaggct ccgcgacagc gcgcgctgcg
      961 gttcggtgcc gacgagcacc tcttcgatga gagccgcagc gtcgagcacg agccccaccc
     1021 tttgatcgcc gagctcggtc gcgccagcga agcctcgcac gccgcgcagt gagcgcccga
     1081 gcggcttgat cacgatgtct tgctgcccgt acagatggtc cacgacgagc cccaggcgcc
     1141 ggctgccgag cgcgatcacg acgacgaacc cctgccgggc cggcgcttcg ggagcgcggt
     1201 cgaggttgag taagcgagac agccggcaaa tcggtaatgt cgagccgcgc agggtgacca
     1261 cttcgcggcc gtcgaggacg cgcacgctcg actcgtcgag gctgagcgcc tcggagacgc
     1321 tggcgagcgg caacgcgaac acctgcgcct cggcgcgcac gatgagcgcg ctcacgatcg
     1381 cgagcgtgat cggcaacgtg atcgtcatct tcgtgccgat gccaggctcg ctgtgcacgt
     1441 cgatgacacc gccgagcttg ccgatgttcg tcttgacgat gtccatgccg acgccgcgac
     1501 ccgagacgtc gtcggcactt tgtcgcgtgg agagccccgg caagaagatg agcccgagga
     1561 tctcttctct cgcgagctct ccgacctcgg tgcgctcgac ttttccgagc tgcaccgctc
     1621 gctccacgat cgcgctctcg tcgattccgc gcccgtcgtc ttcaacctcg atcatcacgt
     1681 ggttgccctt ttggtaggcg ttcaacgcga tggtgccggc ggcgggcttg ccgacctggg
     1741 cgcgcttgtc cgacgactcg atgccgtgat cgatggcgtt gcggatcatg tgcatcaacg
     1801 gatcgctcag ctcctccacg atgagcttgt cgatttcggt ttcggcgccg gtgatgacga
     1861 ggcgaatctc cttgtggagc tcgcggctga tctgccgaac cacgcgcgcc aatcgctcga
     1921 agacttggcc gagcggcacc atgcgcactt cgaggatgcc gtcctgcatt tcgctcaggc
     1981 gtcggtcgaa gttgcgatgc aggcgcatga gctcggccgc gagcttcggc agcccatcgt
     2041 tgcgtacgcg ctcggcggtg cgtgagagcg cgccccgcac gatcgcgagc tctcccacga
     2101 tgttcatcag atggtcgagc ttgcgaatgt cgacgcgcac cgtctgcgtg agcgagcgca
     2161 gcgagcgctc cttttccatc ggaccgccgc ggtccgactc acgcactggt ggaggaaccg
     2221 agtcgcccgc gcgtactggc gcgctcggca tcggcatcgc gagcacgctc gcccgcgccg
     2281 aggtgcgcga aggaccggcg tcttgcgctc gtcggcgcgg gatctcttcg atgacgacgt
     2341 tgtcggcccc gagcgcgccg atgagcgtcg cgaggtcgtc cttcgacgcc atcaaaagat
     2401 ctagctcgat gctatcggcg ctcgtcgtct ctccggtcgg gaggtacgtg atgatttcgc
     2461 cgtgcaggcg cgccttctct ttgatctgct cgagggcgcg atcgatggtc gcgaggtcga
     2521 actgcacgcg cagccgatag agcgacaggc gctgctcgat gttgaccttc agccggtgct
     2581 cttcgtactc ggtgagcacc gcgagcatgc cgggctcgaa ctcgtactca ctcacgacac
     2641 tcgactgctc ggggacgtgc gcgccgagcc gctcgagctt ggcgagcagc gcatctgcct
     2701 ccggcagcgg ctcgtcgatg ccggctttct cctgctcgag caccttgttg taggcgtcga
     2761 cggcggagaa caagacgtcg agcgtgtcgg cggcgagcgc catgcgtccg agccgcagcg
     2821 cgtcgagcag atcttcgagc cggtgcgaca aagcgccgag ccgcgtcgca ccgaagagcc
     2881 ccgcgagccc cttcagcgtg tgcacggccc ggaacgcctc gttcacgagg gacggatccg
     2941 gcgtgccctg cttcgacgcg ctgtcgagcg acagcaagtt gcgcgacagg gtctctatga
     3001 tctcctgcgc ttcggaaaag aactcctctc gtgccttgtc ggaaatctca gccatgcggg
     3061 ctggccttca cacgaggccg ctcgagctgc tcgagagcgg ctttgcgcaa gagttcttcc
     3121 gtgaaaggtt tgggcaggta ggcgtccgcg ccgagcgcga ggccgcgctg ctgatctctt
     3181 tccgaagact gcgtcgagat gagcaggatg ggcgtctttt ggtgctgctc gctgccacgg
     3241 atgaagcgca cgagctcgag gccattgatg tcgggcatgt tgatgtccgt aatcaccagt
     3301 gcatacggcc cacgcggcag cagcctgaga gcatcgaacc cgctcgaggc ctcgacgacc
     3361 gacaattcgc ccgcgctcga gacctggtcc tccagcgccg cacgaatgaa ggctcgcatg
     3421 gtgggagaat cttcgacgac gaggatgcgg ggcatgattg acgggctttc gtccgccaac
     3481 ctcgtaaaaa cggctcgagg gcccgcggac gcgagactca cagactaaac cagccagacg
     3541 cgcttgtcct cgagaaccat cgctcgccaa atgtgttcaa gttactgaca tccctcgtag
     3601 tttgcagaat cgccctgaaa cgatacgagc gagctccggg gagctcccgg cacgacttgc
     3661 tttcctgcgc cgctgaccag ctttgcccgg acggcctgac tggcgcgtcg tcgacctctg
     3721 cgtccggaca ctgactgttt ccagcggcgg cggcggcttt gcacgataac cggcgagccg
     3781 atcttgcaac gtagcgggat gggttcaaag gttcgcccgc aaggatgaca ggctcctcct
     3841 caccctatcg aagatcgcca cgcgtctgcg tgatcggcgc gggcgcgagc gggctcgtca
     3901 gctgcaaagc cctggaagac gcgggcgtcg cgtacgagtg cttcgaggcg agcgatagag
     3961 tgggcggact ctgggttttc aagaatccaa acggcaagag cgcggcttat cgctcactgc
     4021 acatcaacac gtcgcgagag cgcatgcagt ttcgcgatta tccgatgccg cgcgactacc
     4081 cggactatcc cgagcatcgg ctcatcgcac gctatctcga aagctacgcg gaccattttg
     4141 gtctcgcgtc gcggattcgc ttcaacacca ccgtggagcg agtggcaccg cggcctcgag
     4201 gcggattcag cgtaacgctg catggggcag agactcaggt cttcgacgcg gtcgtagtcg
     4261 cgaacggcca ccattgggac ccacgttggc ccgagccacg aattccgggc tcgttcgacg
     4321 gactcgagct gcactcgcac cagtacgtcg acccaaccga gccctacgcg ctcgccggca
     4381 agcgtgtcgc ggtcgtgggc attggcaaca gcgcggtgga catcgcgagc gagcttgcg
//
LOCUS       DASTKS010000845         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_135298, whole genome shotgun sequence.
ACCESSION   DASTKS010000845 DASTKS010000000
VERSION     DASTKS010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135298"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VFQ35_17455"
     CDS             <1..1327
                     /locus_tag="VFQ35_17455"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9932495.1"
                     /translation="NVSVTIYNSNFGLVRETRQVALGTGRVALSYADVSAHIQPESVH
                     IRSLTDASALQVFEQNYRYDLLTPQKLLEKYVGRKLRVARYNEKLGTDEIKEAELLSV
                     EGGPVLRIDGEVVTNFNGRFAFPSLPENLVAKPTLEWLLGSTQPRQSVEVTYLTSNMS
                     WRADYVVSLDAADAHADLNGWVTLENTSGATFMGAELKLVAGDVQRVAPPPPPMAEMA
                     AMDAPVMAPAPPSFRQEGLFEYHLYTLQRPTDLLDKEQKQVSLLEAHGISTKKRLLLR
                     GQPHWYFGRYGAVLDKQKVSAYLVVENSEKNKLGMPLPKGTVRVYKADKTGAQQFVGE
                     DAIDHTPRDEKLEIKLGEAFDVVADQKQTAFNVLGKCANETDWELTLRNHKDNAEVVD
                     VEEPVSGDYSVVQSSHPSARKDANTFTFSIAVPARGEVKVTYRVRVRSC"
     gene            1339..2772
                     /locus_tag="VFQ35_17460"
     CDS             1339..2772
                     /locus_tag="VFQ35_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9932496.1"
                     /translation="MSLPTTIRTSLALLGTLIAFAPARAALGAETVSDERDRKEVSLT
                     VYNSNFALIREVRELAKLGTGRVALEFRDVASTIQPETVAVRASSGGGLRVLEQNYRF
                     DLLTPQTLLEKYVGRNVRAYRYHESTGKEDAVDAKLLSVVEGPILQIGQEITFNYPGR
                     LAFAELPPNLIPKPTLMWLLESQAPKQSVEVTYLAQQLSWHADYVLTVNDAESQGDLV
                     GWVTLTNNSGSSYRNASLKLVAGDVNRVSPVPQRPGARYKTYDAAGASAFSEEALLEY
                     HLYTLDRPADVLDKEQKQVTLIEAPSVGISKKLIFASQPQWFMAQVGPVQTNQKVAVY
                     LDFKNTQANRLGIPLPKGTVRVYKADKAGKKQFVGEDSIDHTPRDEKVRVKVGEAFDV
                     VADRKQMEWHQLGECNAESAWQIELRNHKAEDISVEVREPVNGDFSVVESSLPWKKED
                     ANTFTFDVKVPRGGNAKVMYKVRVKWC"
     gene            3085..3447
                     /locus_tag="VFQ35_17465"
     CDS             3085..3447
                     /locus_tag="VFQ35_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932497.1"
                     /translation="MSQSRKPSLKRRDATGHLDPNYQRDLLQKAREGRVKEERPFVDA
                     PMAADDWAEELGEAFVESATTGEEREAERREESTPEETGGPFVTTTGADEFAAGTDES
                     NIAEATREPFPKTSKADV"
     gene            complement(3565..>3911)
                     /locus_tag="VFQ35_17470"
     CDS             complement(3565..>3911)
                     /locus_tag="VFQ35_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9932498.1"
                     /translation="VNAHATSTPVGDPVELIAARRVFGSAVSDVSISSTKSATGHLLG
                     AAGAVEAIFSLLALRDQVAPATLNLHAPQDADGLDLVPLTPKQRSIRHAMSNSFGFGG
                     TNATLVLSRYDR"
BASE COUNT          813 a         1196 c         1237 g          665 t
ORIGIN      
        1 aaatgtctcg gtcactatct ataactcgaa cttcggcctg gtgcgggaaa cccggcaggt
       61 ggcgctcggg acggggcgcg tcgcgctgag ctacgccgac gtctcggcgc acatccagcc
      121 ggagagcgtt catatccgga gcttgaccga cgcgagcgcg cttcaggtct tcgagcaaaa
      181 ttaccgctat gaccttttga ctcctcaaaa gctgctcgaa aaatacgtcg gtcgaaagct
      241 gcgtgtcgcg cgctacaacg aaaagctcgg aacggacgag atcaaagaag cggagctact
      301 ctcggtcgaa ggcggtccgg tacttcgcat cgacggcgag gtggtcacga acttcaacgg
      361 tcgtttcgcg ttcccgagcc ttcccgaaaa cctggtagcg aagccgactc tcgagtggct
      421 cctcggcagc acgcagccgc ggcagagcgt cgaggttacg tatctcacct cgaatatgtc
      481 ttggcgagcc gattacgtgg tctcgctcga cgccgcggac gcgcacgcag atctcaacgg
      541 ctgggtcacg ctcgagaata cgagcggcgc aaccttcatg ggcgccgagc tcaagctcgt
      601 ggctggtgac gtccagcggg tcgctccgcc tccacctcca atggccgaga tggcggcgat
      661 ggacgcgccg gtgatggctc ctgctccccc gagcttccgc caagaggggc tcttcgagta
      721 ccacctctac acgctccagc gcccgactga tttgctcgac aaggagcaga agcaagtgag
      781 cttgctcgag gcgcacggta tctccacgaa gaagcgcctg ctcttgcgag gccagcctca
      841 ctggtacttc ggtcgttacg gagcggtgct cgacaagcag aaagtctcgg cgtacctcgt
      901 cgtcgaaaac tccgagaaga acaagttggg gatgcctttg ccgaagggca ccgtgcgtgt
      961 ctataaggcg gacaaaacgg gcgcccagca gttcgtcggc gaagacgcga tcgatcacac
     1021 gccgcgcgac gaaaagctcg aaatcaagct gggggaggcg ttcgacgtcg tcgcggatca
     1081 aaagcaaacc gccttcaatg tcctcggcaa gtgcgcgaac gagaccgact gggagcttac
     1141 gctccgaaac cacaaggaca acgccgaagt cgtggacgtg gaggagcccg tgagcggcga
     1201 ctacagcgtg gtccagtcta gccacccgag tgcgcgcaag gacgccaaca cgtttacgtt
     1261 cagcatcgcg gtgccggcgc gcggcgaggt caaagtgact taccgagtgc gagtgcgatc
     1321 ttgctaacgg agagacccat gagcttaccc actaccattc gaactagcct tgcccttctc
     1381 gggacgctca tcgcgtttgc tccggcgcgt gccgcgttgg gcgcggagac ggtgtcggac
     1441 gagcgagacc gcaaggaggt gagtctcacc gtgtacaact cgaactttgc gttgatccgc
     1501 gaggtccgag agctcgccaa gctcggcacg ggtcgcgtcg cgctcgagtt tcgtgacgtc
     1561 gcctccacca tccaacctga aacggtcgcg gtgagggcga gctcgggcgg cgggctgcgc
     1621 gtcctcgagc agaactaccg gttcgacttg ctgacgccgc agaccctgct cgaaaagtac
     1681 gtcgggcgaa acgtgcgcgc ctaccgctat cacgagtcga ccggcaagga ggacgcggta
     1741 gatgccaagc tcctctccgt cgtcgaaggg ccgatcctgc aaattgggca ggaaatcacc
     1801 ttcaattatc caggacgcct cgcgttcgcg gagctcccgc cgaacctgat cccgaagccc
     1861 acgctgatgt ggctactcga gagccaagcg ccgaagcagt cggtcgaggt gacctacttg
     1921 gcccagcagc tgtcgtggca cgctgactac gtgctcaccg tgaacgacgc ggagtcacag
     1981 ggcgatctgg tcggctgggt cacgctcacc aacaactccg gctcgagcta ccgcaatgct
     2041 tcgctcaagc tcgtcgccgg ggacgtgaac cgcgtctcgc ccgtaccgca aaggcccggc
     2101 gctcgataca agacttacga cgcagcaggc gcgagcgcgt tcagtgaaga ggccttgctc
     2161 gagtatcacc tctacaccct cgaccgcccc gcggacgtgc tcgacaaaga gcaaaagcaa
     2221 gtgactctca tcgaggctcc ttcggtcggc atcagcaaga agctcatctt cgcgagtcaa
     2281 ccgcagtggt tcatggctca ggtcggaccg gtgcaaacca atcaaaaggt cgccgtctac
     2341 ctcgacttca agaacaccca agcgaatcgc ctgggcatcc cgctgccgaa gggcacggtg
     2401 cgcgtctaca aggcggacaa ggccggcaag aagcaattcg tcggtgagga ctcgatcgat
     2461 cacacgccgc gagacgagaa ggtgcgcgtc aaggtggggg aggcgttcga cgtcgtcgcc
     2521 gacaggaagc agatggagtg gcaccagctc ggtgaatgta atgcggagag cgcctggcaa
     2581 atcgagcttc gcaatcacaa ggccgaagac atctcggtcg aggtgcgtga gccagtgaac
     2641 ggcgacttca gcgtcgtcga gtcctcgttg ccctggaaaa aagaagacgc aaataccttt
     2701 acgttcgacg tgaaggttcc gcgcggcgga aatgcgaaag tgatgtacaa agtgcgcgtc
     2761 aagtggtgct gacgcctcga gatccggcac atctcgcgaa cggatgaagc tttcgtctct
     2821 tcgatcgccg cgccctcggc aagaaagggt tgttttcccg cgtttctccg tcgctcacgc
     2881 gtcaggcgcg cttctcgctt gcgttctcgg ctctcgcggc gggcagaaac gtgggccacc
     2941 ccctaatgaa tgatgagacc gacgcacacc tcggaagcgc ccgttctctg aacatttcgc
     3001 agcgaaacaa tgacagctgc tagttgcttc tgctgctgat cgggctattc tcttgcgggt
     3061 acggcccact cgaggagagt tccgatgagt caatctcgca agccgagtct gaagcgacgt
     3121 gacgctaccg gacacctcga tcccaactac cagcgcgatc ttctgcaaaa ggcgcgagag
     3181 ggccgcgtca aagaggagcg gcccttcgtc gatgcaccca tggcggcgga cgactgggca
     3241 gaagagttgg gcgaggcctt cgtcgaatcg gcgaccaccg gtgaagagcg cgaggcggag
     3301 cgtcgcgaag agagcacccc tgaagagacg ggcggcccct tcgtaacgac gacgggagcc
     3361 gacgagtttg cggctggcac cgacgagtcg aacatcgccg aagcaacgcg cgagccgttt
     3421 cccaagacgt cgaaagcaga cgtctagcct cactgcgctg cagcttcgcc gcgctcgcgt
     3481 agaacggcgg agctggccgg tgcaaacgcg acggctgtcg gcagctttgc cgcgctcaca
     3541 tacaccggcg gagctggggc ggcgctatcg gtcgtaccgg ctgagtacca gcgtggcgtt
     3601 cgtgccaccg aagccgaacg agttcgacat cgcgtggcga atgctgcgct gcttcggcgt
     3661 gagcggaacc aaatcgagcc cgtcggcgtc ttgcggagcg tggaggttca gcgtcgcggg
     3721 agcgacctga tcgcgcaggg caagaagaga gaagatcgcc tcgacggcgc cggcggcgcc
     3781 tagaagatgg cctgttgcgg atttggtcga cgaaatggag acgtcgctga cggcgctccc
     3841 gaatacgcgg cgcgctgcaa tcagctccac ggggtcgcct actggcgtcg acgtcgcgtg
     3901 ggcgttcacg t
//
LOCUS       DASTKS010000846         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_803820, whole genome shotgun sequence.
ACCESSION   DASTKS010000846 DASTKS010000000
VERSION     DASTKS010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803820"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFQ35_17475"
     CDS             <1..580
                     /locus_tag="VFQ35_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9932499.1"
                     /translation="FPLVGVDLSRPKPKDPSAFERVLGVPVRFGMGSAALRLAPDAPR
                     IPLKSRDPLLRRTLETMASRLGLGEAAPPLEMVIRGRLRELLPKGKAEASHVASSLAL
                     STRTLHRKLAEVGRTFHDVLSEFRRAEAERLLSRAEAPLSEVALRLGFADQSAFSRAF
                     KRWTELTPSEWRSTHVRRVKSKTGRAGSEEPT"
     gene            577..1965
                     /gene="fumC"
                     /locus_tag="VFQ35_17480"
     CDS             577..1965
                     /gene="fumC"
                     /locus_tag="VFQ35_17480"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET9932500.1"
                     /translation="MTTRIEHDSLGAVEVPADRLWGAQTQRSLEHFRISGERMPIELL
                     RELALVKRAAAIVNQRLGVLDGHVAQAIARAADEAHQGLLDAEFPLVVWQTGSGTQSN
                     MNMNEVLANRASELLGGPRGEARLVHPNDHVNKSQSSNDAFPTAMSMAALRAIDERVV
                     PAVATLRDALSEKAHEFGKVVKLGRTHLMDATPVTLGQEIGGWAHQLTQSLAHLDSTR
                     AHLRELALGGTAVGTGLNAPPHFAEAVASELTRISGLGLKTAPNKFEALAAHDAIVFA
                     HGALKTLAAALFKLANDVRWLASGPRSGLFELSIPENEPGSSIMPGKVNPTQAEAMLM
                     ACAQVFGNDVALNIGGSNGNFELNVFKPLIINAFLQSARLLTDCCLGFRQHLVLGMRP
                     NEKRIAELLERSLMLVTALTPHIGYENAARIARKAHLDGTTLKEAALTLGLVSADDFE
                     RWVRPEQMLGPR"
     gene            complement(2010..2258)
                     /locus_tag="VFQ35_17485"
     CDS             complement(2010..2258)
                     /locus_tag="VFQ35_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9932501.1"
                     /translation="MTEPHTHNCESCSMPIETGRYCKYCVNERGELQSFDVRFERMVA
                     WQERRTPDKSRAEVEAMTLAYMAKMPAWKDHPRVRGAG"
     gene            complement(2255..2719)
                     /locus_tag="VFQ35_17490"
     CDS             complement(2255..2719)
                     /locus_tag="VFQ35_17490"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9932502.1"
                     /translation="MAAEPRSAAREFAGLFPRVYLAFHRRDEKRELPNASRAVLQHLA
                     LTGPLTIGEMAQHLSRAQSVVSEIVDHLERDGLLERMRDARDRRRVLVWLSQSGQAEQ
                     ERANEVLSSELLEHAMQHMTPASREALLSGMQALLDANQQHPPTLKKKGKSR"
     gene            complement(2833..3468)
                     /locus_tag="VFQ35_17495"
     CDS             complement(2833..3468)
                     /locus_tag="VFQ35_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932503.1"
                     /translation="MDWLESYEVRWFFAPEHPVVEDLRQWFGDVPIEHDDRKDHYYFD
                     QRRPDLNAKDRTTGKGSKLEFKYRAGTLGPTHFAPGVVGDLERWVKLSLKLDDDTIEA
                     FPLSVAVQKARRLRKFEYSHGDVTEVPAEKKVMAGCGVEFTAIAASRHAQVEHAVTLG
                     LEAFGPPEAALAALQSTAHQVFQERPALELGAANACSYSTWLIKTFRSGAR"
BASE COUNT          642 a         1221 c         1172 g          629 t
ORIGIN      
        1 tttcccactc gtcggcgtcg atttgtctcg accaaagccc aaagacccga gcgccttcga
       61 gcgcgtgctc ggggtaccgg tgcgcttcgg gatgggctcc gcggcgctgc ggctcgcccc
      121 cgatgcgcca cgaatcccac tcaagagccg cgacccgctg ctgcgccgca cgctcgagac
      181 gatggcgtcg cgcttggggt tgggcgaggc cgcgccaccg ctcgagatgg tcatccgcgg
      241 acgcctgcgg gagctcttgc caaaaggcaa ggccgaggcg agccacgtgg cgagctccct
      301 cgctctctcg acccgcactt tgcaccgaaa gctggccgag gtcgggcgca ctttccacga
      361 cgtcctcagc gaatttcgcc gtgccgaagc cgaacgccta ctctcacgag ccgaagcgcc
      421 gctgagcgaa gtcgcgttgc gactcggctt cgccgaccag agcgccttct ctcgcgcgtt
      481 caagcgctgg accgagctta caccgagtga gtggcggagc acgcacgtcc gccgcgtgaa
      541 atccaaaaca ggaagagcag gatcggagga gcctacatga cgactcgcat cgaacacgat
      601 tcgctcggcg cggtggaggt gcccgccgac cggctgtggg gcgcccaaac ccagcgaagc
      661 ctggagcact ttcgaatctc cggcgaacgt atgccgatcg agctcttgcg tgagctcgcg
      721 ctcgtgaagc gcgcggcggc catcgtaaac caaaggctcg gagtgctgga cggtcatgtg
      781 gcacaagcca tcgcgcgcgc ggcggacgag gcgcatcaag gcctgctcga cgccgagttc
      841 ccgctcgtcg tctggcagac gggtagcggc acgcaaagca acatgaacat gaacgaggtg
      901 ctcgcaaatc gagcgagcga gctccttggc gggccgcgag gtgaagcgcg actcgttcac
      961 ccgaacgatc acgtaaacaa gagccagtct tcgaacgatg cttttccgac cgcgatgtcg
     1021 atggccgcgc tgcgcgcgat cgacgagcgc gtcgttccgg cggtggcgac actacgcgac
     1081 gcgctcagcg agaaagcgca cgagttcggg aaagtcgtta agctcggccg tacccacctg
     1141 atggacgcaa ccccggtgac gctgggccag gagatcggcg gctgggccca tcagctcact
     1201 cagagcctcg cccatctcga ctcgacgcga gcccacctac gcgagctcgc gctgggcgga
     1261 acggcggtcg gcacggggct gaacgctccg ccgcacttcg ccgaagcggt cgcgagcgag
     1321 ctcactcgca tcagcgggct cgggctcaag accgcgccga acaagttcga agcgctcgcc
     1381 gcgcacgatg cgatcgtctt tgcgcacggc gcactgaaga cgctcgcggc cgccttgttc
     1441 aagctcgcga acgacgtgcg ttggctcgcg tcggggccac gctcgggcct gttcgagctc
     1501 agcatccccg aaaatgagcc cggcagttcg atcatgccgg gcaaagtgaa cccaacccag
     1561 gcagaggcga tgctgatggc ctgcgcgcag gtgttcggca acgacgtcgc gctcaacatc
     1621 ggtggctcga acggcaactt cgagctcaac gttttcaagc cgctcatcat caacgcgttc
     1681 ttacagagcg cccgcttgct caccgactgc tgcctcggct tccgccagca cttggtgctc
     1741 ggcatgcgcc ccaacgaaaa gcgcatcgca gagctattgg agcgctcctt gatgctggtg
     1801 accgcgctca ccccgcatat cggctacgaa aacgcggcgc gcatcgcgcg caaggcgcac
     1861 ctcgatggaa ccacgctgaa ggaggcggcg ctcacgctcg ggctcgtctc ggccgacgat
     1921 ttcgagcgct gggttcgccc agagcagatg ctcggcccgc gctgactcac cgctgcgtgc
     1981 ccgggacacg ccgcgagtga gccccgcgct cagccggcgc cgcgaacgcg cgggtggtcc
     2041 ttccaagctg gcatcttcgc catgtaggcg agcgtcatcg cctcgacttc ggcgcggctc
     2101 ttgtcaggcg tgcggcgctc ttgccaagcg accatgcgct cgaagcgcac gtcgaagctc
     2161 tggagctcac cgcgctcgtt gacgcaatac ttgcagtatc gcccggtctc gatcggcatc
     2221 gagcagctct cgcagttgtg ggtgtggggt tcggtcatcg agattttcct ttctttttca
     2281 gggtcggagg gtgttgttgg ttggcgtcga gcaaagcttg catgcccgaa agcagggctt
     2341 cgcgcgaagc cggggtcatg tgctgcatgg cgtgctcgag cagctccgaa gagagcacct
     2401 cgttcgctcg ctcttgctcg gcttgcccgc tctggctcaa ccagacgagc acgcgcctgc
     2461 gatctcgcgc gtcgcgcatc ctttcgagca ggccgtcgcg ttccaagtga tcgactattt
     2521 cgctcaccac tgactgcgcg cgcgagaggt gctgcgccat ctccccgatc gtgagcgggc
     2581 ccgtcaacgc gaggtgctgc agcaccgctc gcgaggcgtt cggcagctcg cgcttctcgt
     2641 cacgtcgatg aaaggccagg taaacgcgcg ggaacagtcc ggcgaactca cgcgcggcgg
     2701 agcgaggctc ggcagccatg ccctgaacgt cgcttttcag ggatgtatcg tcaatcccga
     2761 tgcaatttca gcgtggcccg caatttagcg cttttctggc ccgccgcctc gggccgggac
     2821 cgtcgcgcaa cttcagcgcg cgccgctacg gaacgttttg atgagccagg tcgagtagga
     2881 gcacgcgttc gccgccccga gctcgagcgc cggccgctct tgaaagacct ggtgcgcggt
     2941 ggactgtagc gccgcgagcg ccgcctcggg cggaccgaat gcttcgagtc cgagcgtcac
     3001 ggcgtgttcg acctgtgcgt ggcgagaagc agcgatcgcc gtgaactcga cgccgcaacc
     3061 cgccatgact ttcttctcgg cgggcacctc ggtgacgtcg ccgtggctgt actcgaactt
     3121 tcgtaagcgc cgcgcctttt gaacggcgac cgaaagagga aacgcttcga tcgtgtcatc
     3181 gtcgagcttc agcgagagct tgacccagcg ctccaagtcg ccaaccaccc cgggcgcgaa
     3241 gtgagtgggc ccgagcgtgc cggcgcgata cttgaactcg agcttcgagc ctttgccggt
     3301 cgtgcgatcc ttggcgttca agtcgggtcg acgctgatcg aagtagtagt ggtccttccg
     3361 gtcgtcgtgc tcgatgggca cgtcgccgaa ccactggcgc aaatcctcca ccaccgggtg
     3421 ctccggcgcg aagaaccagc gaacctcgta actctcgagc caatccatgg cgcctccgga
     3481 aaccgattcc ccgaattatc ggggtggggg gcgccgcggg ccaaaatccc tcgatcgaat
     3541 cgagcttcgg caaagcacgt acgcgcacgc gccgggcggg gcgactcgaa agtaaccggc
     3601 tgtttttcct gtagaaaccg aatttgcacg gaggtgccta cccagcgctt cttgcgaccg
     3661 gacg
//
LOCUS       DASTKS010000847         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1026957, whole genome shotgun sequence.
ACCESSION   DASTKS010000847 DASTKS010000000
VERSION     DASTKS010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026957"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1798)
                     /locus_tag="VFQ35_17500"
     CDS             complement(2..1798)
                     /locus_tag="VFQ35_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9932504.1"
                     /translation="MEKLAIGIDLGTTHTALASVPIGAEAATTRVFEIPQLVARGALE
                     SRALLPSCVYFGHESEGALALPWDENRRFAVGEYARVRAAEAAGRVVVSAKSWLCHPT
                     VDRRSPLLPLGAPEDVEKISPVEASFRYLDHLVEAFRARHPELGELGEHDVVITVPAS
                     FDAAARDLTVEAAMAAGLDDATLLEEPQAALYAWIENAGDSFRAHLRPGDLVLVVDLG
                     GGTSDFSLIAAEERDGALELSRVAVGDHILLGGDNMDLALAHAVRVKLSAAGHELDRF
                     QVSALSYACRNAKEQLLTNPSLDSAPIVVAGRGSQLLGSSLRSELSRAEVEALLVDGF
                     FPRVERSATPATRARSALTQLGLPYAADPAITKHLAQFLRRHEAANGERSLHPTAILF
                     NGGVVKAPALRARLLETLNGWLGEAGAAPARVLPGEDPDLAVARGAAYYGRVRKGRGV
                     RIRGGTARSYYVGIESPMPAVPGMEPPVSALCVAPFGMEEGSEASLPPQELGVVVGEP
                     VEFRFFGSSVRRNDHAGTMLDYWQPGELEELSPIEVTLPAEGRTVGDFVPIRLEAQVT
                     AVGTLRLDARPLAPLTPDERWKVELSVRKSPA"
     gene            complement(1798..2370)
                     /locus_tag="VFQ35_17505"
     CDS             complement(1798..2370)
                     /locus_tag="VFQ35_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HET9932505.1"
                     /translation="MTAAPVSFLTRLWLCWVCWFRVLFDGAFAARVLSAESGDDARAP
                     DALAPAPEPKPAPAQREAEANLGVDGALSLLALFQREGRLLDFLQQDVTAFGDAEVGA
                     AARVVHEGCRRALKAHAEVKSLRSEAEGARVSVTASEVRDVKLVGNVGGSAPYRGILR
                     HRGWRIEALRLPSRVGDQDARVVAPAEVEL"
     gene            complement(2456..3385)
                     /locus_tag="VFQ35_17510"
     CDS             complement(2456..3385)
                     /locus_tag="VFQ35_17510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932506.1"
                     /translation="MTDVDEDLEVTLAQNYLARGATANAIDALKRLLSREPEHAVGHA
                     LLGEALLRQRRLHAALHEAELGLSLSPESALCHRVMAAVHVAHRQIHKAREHAQQARQ
                     LLPEDPYTLMQLASIELLDERPAAAIDYAEQARRLAPDDPDAISCVAQAELAKGNLAA
                     AEVAAREALELFPEHADALITMGELLLRRGALSEAREHAIVVLCSSPDHEGALRLLVS
                     IKARQSWFLGLWWRLNAWLTLRPGRAIFVLVLAYLLQRVLVTWTRIEGHPMTSSIVSY
                     TWLGLCIYSWVAPGFYRRALQKELAQVTLRPDF"
     gene            complement(3382..>3784)
                     /locus_tag="VFQ35_17515"
     CDS             complement(3382..>3784)
                     /locus_tag="VFQ35_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932507.1"
                     /translation="NPFWPLLGSMLAGGWLAWPWFALNSAALGKGKRFDTDLAVLGFG
                     VALNAVFVIGVARVLASGWVQHHEYADLVPQALRLTVFYWLFLRQERTYQLFEHFGGV
                     GRNGLFVVMLGAVMRGSVLALLPSNLRMLLG"
BASE COUNT          666 a         1311 c         1264 g          543 t
ORIGIN      
        1 ctcatgccgg tgatttgcgc acgctgagct ccactttcca gcgctcgtcc ggcgtgagcg
       61 gtgcgagcgg tcgcgcatcg agtcgcagcg ttccaaccgc ggtaacctgc gcttcgagcc
      121 gaatcggtac gaaatcgccg accgtgcgtc cctccgccgg cagcgtcacc tcgatgggag
      181 aaagctcttc gagctcgccg ggttgccagt agtcgagcat cgtccccgcg tgatcgttcc
      241 ggcgtacgct cgagccgaag aagcggaact cgaccggctc cccgacgacg acgccgagct
      301 cctgaggcgg caggctcgcc tcggagccct cttccatacc gaacggagcg acgcacagcg
      361 cgctcacggg cggttccatg cccggcacgg ccggcatcgg gctctcgata ccgacgtagt
      421 acgagcgcgc ggtcccgccc cgaatacgca caccgcgacc cttgcgaacg cggccgtaat
      481 aggcggcgcc gcgagccacc gcgaggtcgg ggtcctcacc gggcagaacg cgcgcaggcg
      541 cggcaccggc ctccccgagc cagccgttca acgtctcgag caagcgcgcg cgaagcgcgg
      601 gagctttgac gacgccgccg ttgaagagaa tggcggtggg gtgcagcgag cgctcgccat
      661 tcgcggcctc gtgccttcgc aagaactgag cgaggtgctt cgtgatggcg ggatcggcgg
      721 cgtacggaag cccgagctga gtcagcgcgc tgcgcgcgcg tgtagccgga gtagcgctgc
      781 gctcgacgcg cgggaagaag ccgtcgacga gcagcgcctc gacctcggct cgggagagct
      841 cactccggag agagctgccg agcagctgcg acccgcgacc cgcgacgacg atgggtgcgc
      901 tgtcgagcga agggttggta agcagctgct ccttggcgtt acggcatgcg tagctcaggg
      961 ccgacacctg aaaccggtcg agctcgtgcc cggccgcgct cagcttcacg cgcacggcgt
     1021 gggcgagcgc gaggtccatg ttgtcgccac cgagcaagat gtggtcaccg accgcgacac
     1081 gcgaaagctc gagcgcgccg tcgcgctctt ccgctgcgat gagcgaaaag tcgctcgtgc
     1141 cgccaccgag atcgaccacg agcaccagat cgcctggccg caggtgcgcc cggaacgaat
     1201 cgccggcgtt ctcgatccag gcgtagagtg ctgcttgcgg ctcttcgagg agcgtcgcgt
     1261 cgtcgaggcc agcggccatc gcggcctcca ccgtgagatc gcgcgccgcg gcgtcgaacg
     1321 aagccggaac cgtgatgacg acgtcgtgct caccgagttc gccgagctcc gggtgtctgg
     1381 cccggaaggc ttcgacgagg tggtcgagat agcggaacga cgcctcgacg ggcgaaatct
     1441 tctcgacgtc ttcgggcgcc ccgaggggca gcaagggcga gcgccgatcg acggtcggat
     1501 gacacagcca gctcttcgcg ctcacgacga cgcgtccggc ggcttcggcg gcgcggacgc
     1561 gtgcgtactc gcccaccgcg aaacgtcggt tctcgtccca cggcagcgcg agcgcgcctt
     1621 cgctctcgtg cccaaagtag acgcacgaag gcagcagcgc gcgcgactcc agcgctccgc
     1681 gcgccacgag ctgcggaatc tcgaagacgc gcgtggtcgc ggcctcggct ccaatcggaa
     1741 cgctggctag cgcggtgtga gtcgtgccga gatcgatgcc aattgcgagc ttttccatca
     1801 gagctccacc tcggccggag ctaccacgcg tgcgtcctga tcgccgactc gcgatgggag
     1861 ccgcaacgct tcgatgcgcc aaccccgatg ccgcaagatg ccgcggtacg gcgcgctccc
     1921 gccgacgttc cccacgagct tcacgtcgcg cacttcgctc gccgtcaccg aaacgcgagc
     1981 accttcggcc tcgctgcgca ggctcttcac ttcggcgtgc gccttgaggg cgcgccggca
     2041 gccttcgtgg acgacgcgcg ccgccgcgcc gacctcggcg tcaccgaacg cagtcacgtc
     2101 ctgctgcaag aagtcgagca agcgcccctc tcgctgaaac agggcgagca acgaaagcgc
     2161 gccgtcgacg ccgagattcg cttcggcttc gcgttgggct ggcgcaggct tcggctcggg
     2221 ggcgggcgcg agcgcgtcgg gagcccgagc gtcgtcgccg ctttccgccg acagcacgcg
     2281 agcggcgaac gcaccatcga acaggacgcg gaaccagcag acccagcaca gccacaggcg
     2341 agtgaggaac gagacgggag cagcggtcac gcgcgggagt gtatgctcgg ccggcccgag
     2401 cacaaccttc tattccggtc ccgccgcgcg acctctgggc ctccgcgccc tgccctcaaa
     2461 aatccgggcg cagcgtgacc tgagcgagct ccttttgcag ggctctgcgg tagaaccccg
     2521 gagccaccca cgagtagatg cagagcccga gccacgtgta cgacacgatg ctcgaggtca
     2581 ttgggtgccc ttcgatgcgc gtccaggtca cgagcacgcg ctgaagcagg tacgcgagca
     2641 ccaagacgaa gatcgctcgg cccggccgca gagtcagcca tgcgttgagc cgccaccaga
     2701 gccccaggaa ccagctctgc cgggccttga tgctcacgag cagacgaagc gctccttcgt
     2761 ggtcgggaga ggagcacaag acgacgatgg cgtgttcgcg cgcctcggag agcgcgccgc
     2821 ggcgcagcaa cagctcaccc atcgtgatga gcgcgtcggc gtgctcggga aagagctcga
     2881 gcgcctcgcg cgcggcgacc tcagctgcgg caaggttgcc cttcgcgagc tccgcttgcg
     2941 caacgcacga aatcgcgtcc ggatcatcgg gcgcaaggcg gcgcgcctgc tcggcatagt
     3001 cgatggccgc ggcggggcgc tcgtcgagga gctcgatcga cgcgagctgc atcagagtat
     3061 acgggtcttc tggcaagagc tgccgtgctt gttgggcatg ctcacgcgcc ttgtggattt
     3121 gccggtgggc gacgtgtacc gctgccatca cgcgatgaca caaagcgctc tccggactga
     3181 gcgaaagccc aagctctgct tcgtgcagcg ccgcgtgcag ccggcgctgt ctcaacagcg
     3241 cttcgcccaa gagagcgtgc cccaccgcgt gctcgggctc gcgcgacagc aggcgcttga
     3301 gagcgtcgat ggcgttggcg gtcgccccac gcgccagata gttctgcgcg agcgtgacct
     3361 ccaagtcttc gtccacgtcg gtcatccgag caacatccga agattcgacg ggagcagcgc
     3421 gagaacgctt ccgcgcatca cagcgccgag catcacgacg aacaagccgt tccgtccgac
     3481 tccgccgaag tgctcgaaca gctgataagt gcgctcctgc cgcaaaaaca accaataaaa
     3541 gacggtcagg cgcagcgctt ggggtaccaa gtcggcgtac tcgtggtgct gcacccagcc
     3601 cgaggcgagc acgcgtgcga cgccgatcac gaacaccgcg ttcagcgcca cgccgaaccc
     3661 gagcacggcc agatcggtgt cgaaacgttt gcccttgccg agcgcagccg agttcagcgc
     3721 gaaccacggc cacgcgagcc agccacccgc cagcatcgag ccgagcagcg gccaaaacgg
     3781 gttt
//
LOCUS       DASTKS010000848        20592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_804095, whole genome shotgun sequence.
ACCESSION   DASTKS010000848 DASTKS010000000
VERSION     DASTKS010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804095"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1675
                     /locus_tag="VFQ35_17520"
     CDS             65..1675
                     /locus_tag="VFQ35_17520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932508.1"
                     /translation="MDERAEVAPIAEMGSRAASAETSERARLFELAVSRSPLACMCVS
                     AKTGRYAFVNPAFAELVGRSHAELSEADPYEVWLSATHPDDLEVEREALNRIGKGEIS
                     SYRLDKRIVREGVAHWVTVDLNSLRDDEGRLEYVFAHFRETQAERGAVAARERLEAQL
                     RQAQKLGALGKLAGGVAHDFNNRLVIIMGYAELLKRGLPDESPLVGHADMVLGSAERA
                     SELTRQLLAYSQRQVLSPRSFDLNETVERMRRLLSRLIGDDVQFDCELAAAQPIFSDP
                     GQIEQVIMNLVLNARDAMPQGGRLCLSTADVAPSRAPLDLPRGDYVALSVRDTGTGIE
                     PEVLPHIFEPFFTTKELGKGSGLGLSTVLGIVKQSGGTVEVESRSGEGTSFTLYLPRA
                     TEQPLAPRYVSEAALPASTPFETVLVVDDDDGVRQLMSDVLALRAYRVLNAKNGRHAL
                     GVVEEHAGDIHLLVTDVVMPEMGGLELAQRLRRRYPGIRVLFVSGYSDDPEALTAPKA
                     PHTHFLPKPFAPGELTRLVCAILEQRAL"
     gene            1797..2543
                     /locus_tag="VFQ35_17525"
     CDS             1797..2543
                     /locus_tag="VFQ35_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9932509.1"
                     /translation="MSAPPHVSIVIPVYNEQAILRDAVEELIAELVAEGTAFEIVLSE
                     NGSTDDTVGLARALAQADARVRVLSSPEPNYGLALRSGIISARGDIVLCEEIDWCDAE
                     FRRRAVALIETGKADFVIGSKLLGESRDERPWLRHVASLCYTRLLHALFDFQGTDTHG
                     LKALRRSRLLEVVEACRVEKDVFASELVIRAARSGVRLCEIPVQVREKRPPSINLLKR
                     VPNVLSNLGRLAWAIHVESRVAAADSRGVR"
     gene            complement(2548..3972)
                     /locus_tag="VFQ35_17530"
     CDS             complement(2548..3972)
                     /locus_tag="VFQ35_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932510.1"
                     /translation="MRPTQGALLSAWLFSACGTRFATTAELPAGTGGTSATFGGAPSC
                     LGGFEQRLRVTTVSLGDDIRAKAPAYDLLERDERLAFALDPNGKPRVAWLNNAGNTVH
                     VSSFELDGSYSAPDITFPSSEVGGLVAGGDGFMLLTSGPDPGDALADPNADDTPARRA
                     ALLSRFENGALAFSAPLTGTAQITMAQGNYRRDCTPSELTGKLASDGQRYGAYFAVHG
                     CLGHPHASFYGDKLVYVNDAGEAQSGGFSWVCRISEGLALLPQAGAFTSLCMSEGDPQ
                     PGLNLVRDDDSQLLSSEAHTRGYVGAQFGGVVQLDDGGYVIAWLSRGTTDGGGFAEQN
                     PDIALMRVDRDFSIVTAKTWLTDTPATAEYNLHIAPYGHNRVLLIWESIEVDSVLGET
                     GFGKYAGTFAQLFDANGSALSAAELLPARPSSGDDIARYANGDLGWAFVPATRNYSRP
                     FNANAASAEPRGHVLNLARLAYCE"
     gene            complement(3969..4982)
                     /locus_tag="VFQ35_17535"
     CDS             complement(3969..4982)
                     /locus_tag="VFQ35_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932511.1"
                     /translation="MRIRRTLALAACCVAAAWACRAKGETVALVRPGQAPSVLADAFN
                     RLRAELRLHHFEVAEVDAKPDEPSAERLAIIAEGTRAIASVAFSVRADRALIEMWLLD
                     RVSGEASTRTLEVELDEDAASTLAFRAVDVLTTSFPDVERLEPPRRAPAPAAKPIETP
                     PHPTTLPPRPNFRLRVQGVALYDGRALGFAYGPALGLNRVLGRLELGLAIAGPLLGAR
                     LESIEGTASLRQELAFVDARIDLVRTEAYAVSAALGVGLHFLQAEGRAEPPLRSKNDA
                     VFGALLSAGADAELFVSRSVALTLSLSALALAPKQGVELHDARAELALPLLQSALGMA
                     VGL"
     gene            complement(4986..6242)
                     /locus_tag="VFQ35_17540"
     CDS             complement(4986..6242)
                     /locus_tag="VFQ35_17540"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9932512.1"
                     /translation="MRDRLDDLTALARERAAGEVSREEHSAGRQRLLRRAAQTGAEQK
                     KRRPLLWVFGFCAAAAAIALVFGFVRTRPLRFEVTGAASSTSNYLSAAHDRAADVRFS
                     DGSTIHAEPGSRLRIDQARNDGARVSIERGAATASVKHAERSNWQFLAGPFEVRVTGT
                     RFRLSWDPQAEEVDLVLLEGSVEVEGPLANSHFAVRAGQRFRASLADGSMRVESSIPK
                     PPTAAATSEAPSGQAPPPSTATGDSPLTHTAPRNKVPASAPSNESAHESWPELVRRGK
                     FDAVVRSARSRGIDACLASSSAADVRALADAARYAGQSALATQSLLALRRRFASSSDG
                     NAAAFLLGRVDESSGQLAAADSWYDTYLRESPNGQFASDALAGRMRTVSSLRGTAAAK
                     PLARTYLQRYPNGVHAPAAKKLGGLD"
     gene            complement(6235..6798)
                     /locus_tag="VFQ35_17545"
     CDS             complement(6235..6798)
                     /locus_tag="VFQ35_17545"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932513.1"
                     /translation="MQAVGQGDREALGVVWDRYSPLVRTVLRANLGFDSATEDLLQEV
                     FIVFLRNAAEIRAGGALKAFLTTVAVRVVLVELRRRRVRRWVTLTPTGEIPERAASPS
                     DDEGAAALGGLYRLLDRMPPRRRVAFVLRHVEGLELAEVAEHLKVSESTAKREVLKAR
                     QSIFARAERCEPALWSFLSRLQGRDDA"
     gene            complement(6943..8457)
                     /locus_tag="VFQ35_17550"
     CDS             complement(6943..8457)
                     /locus_tag="VFQ35_17550"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932514.1"
                     /translation="MTVPLELEEIQGLVLSGYGERPVARYALFEVVDAARAKAWLKAL
                     LPELQFSEYRRSRRHQPPFLKPICVNIAFTHAGLSVLGLPAPTLSGFSLPFQEGMHEP
                     SRARRLGDDGESSPENWRWGGPKQRLHGVLLVFAGTEDADPEEWKQVGDFLELHCVAG
                     NGIRTLEVLDTMPANRLLRKEHFGFRDGIANPRVSGVSRPGSSDVVESGEFVLGYENE
                     YGKYPMSPELPASSAKAELLPEARTRAGFRDFGRNGSYLVFRQLSQDVKKLWRYLDTA
                     RRRVPDAPDGWLGAEWVGAKMVGRWRNGTPLTVYPDHPGPQSHEDDNEFSYGEHDDAF
                     GTRCPIGSHIRRTNPRDTALPIPHDPELSGSPEDEAVRTARLSLSNKHRLMRRGRSYG
                     EPLDPHYEPEAMLAADDAAERGLHFLCFNANLGRQFEFVQSNWAQNPAFAGLSSDPDP
                     ITSTQRRFPCDASTFTIPGCPPRRVHGVPRVVEVRGGAYFFMPSRAGLRFLSEL"
     gene            complement(8454..9782)
                     /locus_tag="VFQ35_17555"
     CDS             complement(8454..9782)
                     /locus_tag="VFQ35_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932515.1"
                     /translation="MRQETLTVVARVVDVPALRRGLSLIREDMVGGSGARAFQPRAFE
                     GEALGVHFARLVLVPASQDGAFSDWLAFESNFDTTEADAESARRQHLALLAQHAKESL
                     GLAFAHCEGFPRSGTAAEFADYLEKRLVAATASYQGHVERDLQRIRLEQHLREVILTF
                     FESAESAPKAELFKRVRDHVRFRARVDPRLAGFNVDAEPPPAPDPDRRSERLAHRVEP
                     WVQNIAPALPILNPARLWDIYTWQRSDVEYDYQASQEAWTDEHRRRFVEVSDSEDHGV
                     QNAITHVVPLREGQYRLAILKAAHAYIDRMAKEYFDDIGQLGGIPSIHFAKWLLVDDE
                     KRLLFFSNYDSSWESYLGDFVDQAAIGLNLAWSCCDSYPRTRFLAWGGADDEERFKAW
                     GRAYQEPTQVFYSAYPELSIAAINNNSAIRYGLQARPSAAEIGAWFRRLT"
     gene            complement(9877..11544)
                     /locus_tag="VFQ35_17560"
     CDS             complement(9877..11544)
                     /locus_tag="VFQ35_17560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932516.1"
                     /translation="MLPQSPNRALRAIDFDTQRRVLLALAEKRRFVAVIEQDLDGRIT
                     SWNDAAQRLFGYTSDEAVGRSSLMFHDPREVMSGRAASIFQRAAVTGKWEGRTYQIRR
                     DGTGFWAQLTLLLRRDAQGRAASLISVVRDLSRVERAETEAARSLANGAPLIDSRTEV
                     LVATHADGTIVEASASFCLLLGHPRESLLGSRLADWFEDPAAIDGVLASALRDEEASA
                     AALELWTPPQNLRVELSSCTVAGVEPGATVLLNVLEPPPNWFETASIPSEPVPVARAS
                     TTPPAARVRSSEVTARVGSSLPTAELSHELRTPLTAVIGFAELIRSGQAGPMGLIQRE
                     YMDDIIHSAHHMLRLLDAELESARETYAATVAPVEIGAILNAARSTLRLAASQKNIDV
                     LIDQRCELSLAAESATRLQQIVFNYLSNALKFTGESGRVTLSCHVIDETTLCVEVSDT
                     GSGILPEQQSRLFREFDQLDVDAELRAQGSGLGLAITRRLARSLGGDVAVTSTPGAGS
                     VFSVLLPLKRVSMRVDADDLHESTQVLKAARYSTEGSTGEDDRTLCA"
     gene            11943..13772
                     /locus_tag="VFQ35_17565"
     CDS             11943..13772
                     /locus_tag="VFQ35_17565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9932517.1"
                     /translation="MPTILVVEDNPVTRKLVRVSLTAAGIDVVEASTGSEAIALLGER
                     NVDLILQDLALADMDGFELLGKLRKSRGSELPQVVAFTGLADRQRVLAAGFSDLLLKP
                     IDPARLVGYVRERLNAGPTRTRSSPRAARVGSDAMLQSPLWSSVSKLLANLADLSGGA
                     AGFDDVLRSALASFLDSSGFQRGAAYLPAPSGSVALAAQLGFHGVQLERLSDFAGRVA
                     LLDRVMVTTEPLVIDSVVVPDDARLLADLEAHSLLIVPILHGRTPNAVFVAVSGNRGV
                     APTWLEVGRLMAAPIGQSLALAASVARLAASEHRFRGIAESTRDGIVVADPENIVTYA
                     NASAARVLSRPHAELIGAQVKDIVPFVADGAFAGRVRLPGGRDVPLEVSLQDFEDPPG
                     HLNHVYVLRDLSERLRLEELAWLANHDPLTGLVNRRRFEEELNARLAESQRYGTGGAL
                     LAIDLDQFKPINDTHGHAAGDLVLRAIAEVLKSVTRATDLAARIGGDEFAVLLNHTNA
                     SGADACARKLLERISEHVTSYEGVELRVGASVGVALLPEDGTTREELFAAADRALYVS
                     KQSGRRRVTFSGIPQFGKPSEVPPESRSSRERSVLPPRGERRG"
     gene            13822..14556
                     /locus_tag="VFQ35_17570"
     CDS             13822..14556
                     /locus_tag="VFQ35_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932518.1"
                     /translation="MRWLLSSSVFAFSVFSSTLGCAERHASVERPPSESSVLPAAPAS
                     ATSAVATPSASTAPSAQPLPAKEASAAEVPAPKAPAEAEAEAEPPLTGPDGKTLPQTE
                     ERPSIESPAFKRRIERLAQCILRDDPSICSSSFFPLLAYREVKAVEKPERDYQYRLVR
                     HFERDIHEYHKALGDNAKFEGIDVPEASARWMKPGSEGNKLGYYRVLRSRLRFTKSNG
                     KAQNFELTSMISWRGEWYVVHLHGFE"
     gene            complement(14575..15774)
                     /locus_tag="VFQ35_17575"
     CDS             complement(14575..15774)
                     /locus_tag="VFQ35_17575"
                     /inference="COORDINATES: protein motif:HMM:NF022738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HET9932519.1"
                     /translation="MPPVAFEPQPDDVEFFARLPEITDFAHVTERSAYHAAPASWHIV
                     ITDVRGSTRAIEAGRYRDVNALGVASIVAVCNALPSVQLPYVFGGDGATLLVPGAHLA
                     ACARALRGVAQVAVSAYELELRTGVVPVAALYDEGHAVRVARFRPSPHVCLAMFAGSG
                     FATAERWIKDSSRAASYSIPPSDDANASFEGFECRWQPVESKQGSIVSLLVQALARDE
                     AAQAEVYRSVLGALAGIHGQDEARPVSVERLKLKAFGGDYSTEARLLSRDTSGREYDR
                     AHQNAQKKARAGRVLMGLGASAGGFDGGNYASELVENTDFRKFDETLRMVLDLSEDEI
                     GKLEEFLEAERSAGRLVYGLHRAPSALVTCFVRSYAGNHLHFVDGANGGYALAAKQLK
                     SQLAGKG"
     gene            15881..16942
                     /locus_tag="VFQ35_17580"
     CDS             15881..16942
                     /locus_tag="VFQ35_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932520.1"
                     /translation="MKASRGCVALLTLLCAKTVFAQSPAPATPGAGPATPGAGPASPG
                     AGPASPATGPATPGAGPATPGAGPASPATGPASPATGPAPVSAAPPGEIILTPPPSAP
                     SEPPAAPPTAPPVEPPPSAPPSIEPQLEVKRRATTPMPPVSGKPHDFNIELGFGVNGR
                     FGQASGYSDDEGLGAAYGAGFWFGLSDAVALGLELARIDLGRASAHSGQNFVTAEYAS
                     TGASLGGRFFAFQTEHLRLFAALRVGLALEHVSAYGVRALGTPLDPATSFNCSGTSGP
                     ALGFGAGIGGVFHLNSHLDFVSRLDGHVEQLTSDVVNGCAAGVGSTASLLLGVGLAYG
                     FDGPASSGDPYAARRGNKF"
     gene            complement(17081..17317)
                     /locus_tag="VFQ35_17585"
     CDS             complement(17081..17317)
                     /locus_tag="VFQ35_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932521.1"
                     /translation="MVGVVVAAVGVLMSGVTGSGVIGVTVSGVIVSGVIVSGVIVSGV
                     IVVLSVIVSRVSVILIVSRVSVIVGGVSVIVLAL"
     gene            17366..18358
                     /locus_tag="VFQ35_17590"
     CDS             17366..18358
                     /locus_tag="VFQ35_17590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9932522.1"
                     /translation="MRIRPAGGHGHGSHGHGGHGHGGHGHGGHGHSGAALRALVLALV
                     ATGTFRLVEAGVGLWSGSLALLADAGHMLADAGALALALAAQRFATRPRTERSTFGYR
                     RAEVLAAFVNGMALAGVAVLIVKEAIERWVSPHEMNGRAVLLTAFLGLCVNLIVAAIL
                     MRGQKDNINLRAAFAHVLMDAVGSVGAMLAGLAVTFLGWYRADPLLSAGIAVLVAVSG
                     FRILKEAASILLESAPPHLDVAEIEKTIMSCDGVSEVHDLHVWRISERFDTLTAHVVL
                     KRGKHGTDVCRHVAEQLKSAYGLDHVTIQPEPPRPDDLVSLRRSRDGGPLRNAG"
     gene            18614..19006
                     /locus_tag="VFQ35_17595"
     CDS             18614..19006
                     /locus_tag="VFQ35_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CesT family type III secretion system chaperone"
                     /protein_id="HET9932523.1"
                     /translation="MIQASQDLETFLGRLERPFERADSGTYVIRMGAGGALVALRIEP
                     PVLVIRVEIGRVPSSPQASLSLFKKLLELNASSMLHSAYGLEGDRIVLAAALELSSLD
                     LNELEAVLSDIDLALAEHVSTLHELVKS"
     gene            19019..19834
                     /locus_tag="VFQ35_17600"
     CDS             19019..19834
                     /locus_tag="VFQ35_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET9932524.1"
                     /translation="MGIFSRLASLIKANINDLINRSEDPEKMLNQIVIDMGEQLIEAR
                     KQVASSIADEKRLERQAQEEGAKAAEWERRAMLAVRAGDENLAKEALARKKEHETLRA
                     QFQEQWQKQNQAVEQLKLALRALNNKIEEAKRKKNLLIARKKRAEAQRSISETMSGLK
                     NASAFEAFDRMASNIERMEAEAEATSELHEQFTGDTLQHKFSELEAAHGVDEDLLALK
                     RKMGLAPPEAPPAAAPVRVEAAPQGVDAAEQEELARALAELDAEQNEPLRMKR"
     gene            20049..>20592
                     /locus_tag="VFQ35_17605"
     CDS             20049..>20592
                     /locus_tag="VFQ35_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932525.1"
                     /translation="MALLVSATDAGATGRGFVAEPRQGEKIRIDGDLREWPAKMTILA
                     ESREGSAIDARAVIGYDDANVYLALRAADKRIARTAQGGDSEDHATLTLAFPRGRGEY
                     SIYPVQIYPGSPGRLPALVKLGGKAVSGAKAVENATDKELFLEAQIPWTTFPEAAKLR
                     VGLRAAVSYTNADAPGSVKGI"
BASE COUNT         3441 a         6859 c         6757 g         3535 t
ORIGIN      
        1 agggcgctcg gcagctagag caaatccgcg cgctgaggca ctctgcgctc tggtaaggtg
       61 cgccatggac gagcgtgccg aagtcgcccc catcgcggag atgggctcgc gcgctgcttc
      121 ggcggagacg agcgagcgcg ctcgtctctt cgagctcgcc gtatcgaggt cgccgctcgc
      181 gtgcatgtgc gtgtcggcga agaccggtcg ctacgctttc gtcaacccag cgttcgcgga
      241 gctcgtcggt cgctcgcatg ccgagctcag cgaggcggac ccttacgaag tttggctcag
      301 cgcgacccac cccgacgacc tcgaggtcga gcgcgaggcg ttgaatcgca tcggcaaagg
      361 cgagatctcg agctaccgcc tggacaagcg catcgttcgc gagggagtag cgcactgggt
      421 taccgtcgat ttgaacagtc tgcgcgacga cgagggtcgg ctcgagtacg tgttcgcgca
      481 cttccgcgag acgcaggcgg aacgtggcgc agtggctgcg cgtgagcgct tggaggcgca
      541 gcttcgacaa gcgcagaagc ttggcgcgtt gggaaagctc gccggcgggg tggcccacga
      601 tttcaacaac cggctcgtga tcatcatggg ctacgccgag ctcttgaagc gtggcttgcc
      661 ggacgagagc ccgctcgtcg gtcacgctga catggtgctc ggcagcgccg agcgcgcttc
      721 ggagctcact cggcagctcc tcgcctacag tcagcgccaa gtgctctcac cgcgttcgtt
      781 cgatttgaac gagacggtcg agcgaatgcg taggttgctt tctcggctca tcggcgacga
      841 cgtgcaattc gattgtgaac tcgcggccgc gcagcccatc ttctcggatc ctggtcaaat
      901 cgagcaggtg atcatgaacc tggtgctcaa cgcgcgcgac gcgatgcccc agggcggccg
      961 cttgtgcctc tctacggccg acgtcgcacc ttcgcgcgcg ccgctcgatt tgccccgcgg
     1021 agactacgtc gcgctcagcg tgcgcgacac cggcaccggc atcgagccag aggtgttgcc
     1081 gcatatcttc gagccgttct tcacgacgaa agagcttggc aagggtagcg gcctcggcct
     1141 ttcgacggtg ctcggcatcg tgaagcaaag cggaggcacg gtggaggtcg agagccgttc
     1201 gggcgaaggg accagcttca cgctgtacct cccgcgcgct accgagcagc cgctcgcgcc
     1261 tcgctatgtc tccgaggccg cgctgccggc ttcgacacct ttcgaaacgg tgctcgtcgt
     1321 cgacgacgac gacggcgtgc gtcagctgat gagcgatgta ttggcgctcc gagcgtaccg
     1381 cgtcctcaac gcaaagaatg gtcgccacgc gctcggcgtc gtagaggagc acgcgggcga
     1441 catccacctg ctggtgaccg acgtcgtcat gccggagatg ggcggcctcg agctcgcgca
     1501 acggttacgg cgccgctacc cagggatccg cgtgctcttc gtttccggct acagcgacga
     1561 cccggaggcc ctgaccgcgc ccaaggctcc ccacacccat ttcttgccca agccgttcgc
     1621 tcccggagag ctcacgcgcc tggtttgcgc gatcctcgag caacgcgcgc tttgaaacga
     1681 ccgccggaac ggccaagcgc cgcggttgca ctgggtgtag ccgacgcgcg aagggaaacc
     1741 ggattgccgg tgcgttctct tccgctatcc tccgccgcct gtcgctcgac ccaccgatgt
     1801 ccgcgccgcc ccacgtctcg atcgtgatcc ccgtctacaa cgagcaggcg atcctgcgcg
     1861 atgcggtcga agagctcatc gccgaactgg tagcagaggg gacggcgttc gagatcgtgc
     1921 tctccgagaa cggttcgacc gacgacacgg ttggcctggc aagagctctc gcgcaggcgg
     1981 acgcacgcgt gcgagtgctg agctcgcccg agccgaacta tggcctcgcg ctgcgcagcg
     2041 gtatcatcag cgcgcgcggc gacatcgtgc tgtgcgagga gatcgactgg tgcgacgcgg
     2101 agtttcgtcg acgcgcggtc gctctcatcg agaccggcaa ggccgacttc gtgattggct
     2161 cgaagctcct cggcgaatct cgggacgagc ggccttggct gcggcacgtg gcgagcttgt
     2221 gctacacgcg cctgctgcac gcgctgttcg atttccaggg gacggacacg cacgggctca
     2281 aggcgctccg gcgaagccga ttgctcgagg tggtcgaggc gtgccgtgtc gaaaaggacg
     2341 tgttcgcgag cgagctcgtg atacgggctg cccgctcggg cgtgcggctc tgcgaaatcc
     2401 cggtgcaggt gcgcgagaag cgccctcctt cgatcaacct gctgaagcgg gtgccgaacg
     2461 ttctctcgaa cctgggtcgg ctcgcctggg ccattcacgt cgaatcacga gtggcggcgg
     2521 ctgattcgcg cggggtccgc tgagctttca ctcgcagtac gccaggcgag cgaggttcag
     2581 tacgtggccg cgcggctctg cgctcgccgc gttcgcgttg aaaggccgcg agtagttgcg
     2641 cgtagccggc acgaacgccc aaccgagatc tccgtttgcg tagcgcgcga tgtcgtcgcc
     2701 gctgctcgga cgcgcgggca agagctcggc ggcgctgagc gcactgccgt tcgcgtcgaa
     2761 gagctgcgcg aaggtccctg cgtacttgcc gaaccccgtt tcgcccagaa cgctgtccac
     2821 ctcgatgctc tcccaaatca ggagcactcg attgtgaccg taaggggcga tgtgaaggtt
     2881 gtactccgcc gtagcgggcg tgtcggtcag ccacgtcttc gcggtaacga tcgagaagtc
     2941 gcgatccacc cgcatcagcg cgatatccgg attttgctcg gcaaaaccac cgccgtcggt
     3001 cgttccgcgc gagagccaag caatgacgta gccgccgtcg tcgagctgca ccacgcctcc
     3061 gaactgcgcg cccacgtagc ctcgggtatg ggcttccgac gagagcagct gcgagtcgtc
     3121 gtcgcgcacc aggttcaagc cgggctgagg gtcaccttcg ctcatacaga gtgaagtgaa
     3181 cgctcccgct tggggtagca acgcgaggcc ctcgctgatg cgacacaccc atgagaagcc
     3241 tcccgattgg gcttcgcccg cgtcgttcac gtacacgagt ttgtctccgt agaaggaagc
     3301 gtgcgggtgc ccgaggcagc catgcaccgc gaagtacgcg ccgtatcgct ggccatcgct
     3361 cgccagcttg ccggtgagtt cgctcggagt gcagtcgcga cggtagttcc cttgcgccat
     3421 cgtgatttgc gcggtgcccg tgaggggcgc cgaaaatgca agagcgccat tctcgaagcg
     3481 cgacaagagc gcggctcggc gcgccggagt gtcgtcggcg ttgggatcgg cgagtgcgtc
     3541 gccggggtca gggccactcg tgaggagcat gaagccgtct ccgccggcga ccaggccgcc
     3601 cacctccgag ctcggaaacg tgatgtccgg agcgctgtaa ctaccatcga gctcgaagct
     3661 cgatacgtgg acggtgttgc ctgcgttgtt gagccaggcg actctcggct tgccattcgg
     3721 atcgagcgcg aaggcgagtc gttcgtctcg ctcgagcaga tcgtaggcgg gcgccttggc
     3781 gcgaatgtcg tcccccaggc tcaccgtcgt gactcgcagg cgttgctcga agccgccgag
     3841 gcaactggga gcgccgccga acgtggcgct cgtcccgcca gttcccgccg gtaattcggc
     3901 tgtggtcgcg aagcgagttc cgcacgcgga gaagagccaa gctgagagca gcgctccctg
     3961 ggtcggcctc atagtcccac cgccatcccc agcgcggact gcagtagcgg tagagcgagc
     4021 tccgcgcgcg cgtcgtggag ctcgacccct tgtttggggg ccagagcgag cgcactcaac
     4081 gagagcgtca gcgcgacgct tcgcgagacg aagagctcag cgtcggcacc ggcgctcagg
     4141 agagcgccga atacggcgtc gttcttcgag cgcagcggcg gctccgcgcg cccctccgct
     4201 tgcaagaagt ggagcccgac gccgagcgca gcgctcaccg cgtacgcctc ggttcgcacc
     4261 aagtcgatgc gcgcgtcgac gaaggcgagc tcctgtcgaa gagatgcagt cccttcgatg
     4321 ctctcgaggc gagcgcccag gagcggaccc gcgatcgcga gaccgagctc gagccgaccg
     4381 agcacacgat tcagtccgag cgccggcccg tacgcgaacc cgagggctcg gccgtcgtaa
     4441 agcgcgacgc cttggacgcg cagccgaaag ttggggcgag ggggtagcgt cgtcgggtgc
     4501 ggcggtgttt cgattggctt cgcggcagga gccggcgctc gtcgcggcgg ttcgaggcgc
     4561 tcgacgtccg gaaagctggt cgtcagcacg tcgaccgcgc gaaatgcgag cgtgctcgcg
     4621 gcgtcctcgt cgagctcgac ttctagcgtt cgagtactgg cctccccact cacgcgatcc
     4681 aaaagccaca tttcgatcaa cgcgcgatct gcgcgcacgc tgaacgcaac actcgcgatg
     4741 gcgcgtgtcc cctccgcaat gatcgccaag cgttcggcgg agggctcatc gggtttcgca
     4801 tcgacttccg ccacttcgaa gtggtgcaag cgaagttcgg cgcgcaggcg attgaaggcg
     4861 tccgcgagca cgctcggcgc ttggcccggt cgcactagcg cgacggtctc gcccttcgct
     4921 cggcacgccc acgccgccgc gacgcaacag gctgcgagcg cgagggtcct gcgtatgcgc
     4981 atctttcagt cgagtccgcc gagcttcttc gctgcgggcg cgtgtacgcc gttagggtag
     5041 cgttgcaggt acgtgcgcgc tagaggcttg gcggctgccg tcccgcgcaa gctcgagacg
     5101 gtgcgcatgc gacccgccaa ggcgtcggaa gcaaactgtc cgttcggcga ttcgcgcaag
     5161 tacgtgtcgt accacgagtc cgcggctgcc agctgcccgc tcgattcgtc gacgcggccc
     5221 agtagaaaag ccgcggcgtt cccgtcgctg ctagacgcaa accgccgccg cagcgcgagc
     5281 aggctctgcg tcgcgagcgc gctttgcccc gcgtagcgcg cggcgtctgc cagggctcgc
     5341 acgtcggccg cgctcgagct cgcgaggcaa gcatcgatcc cccggctgcg cgccgagcgg
     5401 accaccgcgt cgaacttgcc gcgccgcacg agctcaggcc acgactcgtg agcggactcg
     5461 ttcgaaggcg cactcgcagg caccttgttg cgcggggcgg tgtgggtgag cgggctgtcg
     5521 cctgttgccg tcgacggcgg tggcgcttgc cccgagggcg cttcgctcgt tgccgccgcc
     5581 gtcgggggct tggggatact tgattcgact cgcatcgagc catcggcgag tgaggcgcga
     5641 aagcgctggc cggctcgaac cgcgaagtgg ctgtttgcga gcggaccctc gacctccacc
     5701 gagccctcga gcagcacgag gtcgacctct tccgcttgcg gatcccagct caacctgaac
     5761 ctcgttcccg tcacgcgcac ttcgaacggc cccgccaaga actgccaatt cgagcgctcg
     5821 gcgtgcttca cgctcgcagt cgcggcaccg cgctcgatcg agacgcgggc cccgtcgttc
     5881 cgagcctgat cgatgcgcag gcgcgacccc ggctcggcgt gaatggtcga accgtcgctg
     5941 aagcgcacgt cggccgctct gtcgtgcgcc gcgctcaagt agttcgacgt cgacgaggct
     6001 gctcccgtga cctcgaaacg aagtggtcgc gttcgaacga atccgaaaac cagcgcgatc
     6061 gcggcggcag ccgcgcagaa gccgaacacc cagagcaagg gtctgcgctt cttttgctca
     6121 gcgccggttt gtgccgcccg tcggagcagg cgctgtctgc ccgccgagtg ctcctctcgg
     6181 ctgacttcgc cggcggcccg ctcgcgcgcg agtgccgtga ggtcgtccaa gcgatcacgc
     6241 atcgtcgcgc ccctgcagtc gagagagaaa gctccaaaga gcgggttcgc agcgctcggc
     6301 tcgcgcgaag atgctctgcc gcgccttcag aacctcgcgc ttggccgtcg actccgacac
     6361 cttcaagtgt tcggcgacct cggcaagctc caggccttcg acgtgtcgca ggacgaacgc
     6421 gactcggcgc cgcggcggca ttcgatcgag cagcctatac agcccgccga gcgctgccgc
     6481 gccctcgtcg tcactcggcg aggcggcgcg ctcggggatc tcgccggtgg gcgtcagcgt
     6541 cacccaacgc cggacgcggc gtcttcgaag ctcgaccaac acgacacgta cggcgaccgt
     6601 cgtgagaaag gccttgagtg ctccaccggc gcgaatctcg gcggcgttgc gcaagaagac
     6661 gatgaacacc tcttgcagca ggtcttccgt cgccgaatcg aacccgaggt ttgctcggag
     6721 cacggtgcga accaaggggg aataacggtc ccagaccacc ccgagcgcct cgcggtctcc
     6781 ctgccctact gcttgcacga gctccgcgtc gccgacccgc tccaaaggga agcgcgatac
     6841 tggacgtttt atcagggagg caaccgactg ccgcacgttg tttaaagaga cctgagcccg
     6901 gcggcgcggg ccatcaaaag cggcgcggcg cacccggggt gcttacagct cgctcaggaa
     6961 tcgcagtccc gcgcggctcg gcatgaaaaa gtacgctccg ccgcggactt ccacgactcg
     7021 gggcaccccg tgcactcggc ggggtggaca ccccgggatc gtgaacgtcg aggcatcgca
     7081 agggaaccgg cgctgggtcg aggtgatcgg atccggatcg ctgctgagac cggcgaaggc
     7141 ggggttctgg gcccaattcg actggacgaa ctcgaattgg cgaccgaggt tcgcgttgaa
     7201 gcacaagaag tgaagaccgc gctcggcggc gtcgtcggct gcgagcattg cctccggttc
     7261 gtagtgcggg tcgagcggct ccccatacga ccgaccgcgt cgcatcaagc ggtgcttgtt
     7321 gctgagggag agcctcgcgg tccgcacggc ctcgtcttcg gggctcccgc tcagctccgg
     7381 gtcgtgcggg atcgggagcg cggtgtcgcg cgggttcgtc cttcgaatgt gagagccgat
     7441 tggacagcgc gtcccgaagg cgtcgtcgtg ctcgccgtat gaaaactcgt tgtcgtcctc
     7501 gtggctctgc ggacccggat gatccgggta aacggtgagg ggagtgccgt tgcgccagcg
     7561 gccgaccatt ttggctccca cccactcggc gccgagccag ccgtcgggcg catctggaac
     7621 gcgacgacgg gccgtgtcga ggtaacgcca aagcttcttc acgtcctgtg acagctggcg
     7681 aaacacgaga taactcccgt tgcgcccaaa gtcgcggaag cctgcgcgcg ttcgcgcctc
     7741 cggcaaaagc tctgccttcg cgctcgatgc cgggagctcc ggagacatcg ggtacttgcc
     7801 gtactcgttc tcgtagccga gcacgaactc gcccgactcg accacgtcgc tgctgccggg
     7861 gcgcgacact ccgctcaccc tggggttcgc gatgccgtcg cgaaagccga agtgctcttt
     7921 tcgcagcaaa cggttagccg gcatcgtgtc gagcacctcc aaagtgcgaa ttccattccc
     7981 ggctacgcag tggagctcga ggaagtcgcc gacctgcttc cactcctctg gatcagcgtc
     8041 ttcggtgccc gcgaacacga gcaacacgcc gtgcagccgc tgcttgggac cgccccagcg
     8101 ccagttctcg ggtgagctct ccccgtcgtc accgagcctg cgggcgcggc tcggctcgtg
     8161 catcccttct tgaaaaggca agctgaagcc cgacagcgtc ggcgcaggga gccccagcac
     8221 cgaaaggccc gcgtgggtga acgcgatgtt cacgcaaatc ggcttcagga acggaggttg
     8281 atgccgacgc gaccttcgat attcgctgaa ctggagctcg ggcaagagcg ccttgagcca
     8341 agccttcgcg cgcgcggcgt cgacgacctc gaagagggcg tagcgtgcga ccgggcgttc
     8401 accgtagccg ctgaggacga ggccctgaat ttcctcgagc tcgaggggaa ccgtcacgtc
     8461 aacctccgaa accaagcccc gatctctgcc gcgctcggtc gcgcttggag accgtagcga
     8521 atcgcgctgt tgttgttgat ggcggcgata ctgagctccg ggtacgcgct gtagaacacc
     8581 tgcgtgggtt cctggtaagc gcgcccccaa gctttgaatc gctcctcgtc gtcggcgccg
     8641 ccccaagcca aaaagcgcgt gcgcggatag ctatcgcagc atgaccacgc gaggttcaaa
     8701 ccgatcgcgg cctgatcgac gaagtcgccg agatagctct cccagctgct gtcgtagttg
     8761 ctgaagaaca ggagccgctt ttcgtcgtcc accagcaacc acttcgcgaa atggatactc
     8821 ggaatgccgc cgagctgtcc gatgtcgtcg aagtactctt tcgccatgcg atcgatgtag
     8881 gcgtgtgcag ccttcagtat cgcgaggcgg tactgaccct cccgtagcgg taccacgtgg
     8941 gtgattgcgt tttgtacgcc gtgatcttca ctgtcggaga cctcgacgaa gcgccggcgg
     9001 tgctcgtcgg tccacgcttc ttggcttgct tgatagtcgt actcgacgtc acttcgctgc
     9061 caagtgtaga tgtcccaaag ccgcgcgggg ttcagaatgg gcagagccgg agcgatgttt
     9121 tgcacccacg gctcgacgcg atgtgcgagg cgttcgctgc gtcgatcggg gtcgggcgcc
     9181 ggaggcggct cggcgtcgac gttgaacccc gcgagccgcg ggtcgacgcg cgcacgaaag
     9241 cgaacgtggt cgcgaacgcg cttgaaaagc tccgccttcg gcgcgctctc ggcactctcg
     9301 aagaaggtca agatgacctc tctcaagtgc tgctcgagcc gaatgcgctg gaggtcacgc
     9361 tcgacgtggc cttggtagct cgcggtcgca gcgacgagcc gcttctcgag gtagtcggcg
     9421 aactcggcgg cggtgccact gcgcggaaac ccctcgcagt gagcgaacgc caagccgagg
     9481 ctctccttcg cgtgctgggc gagcagagcc aggtgctgcc gccgcgcgct ctccgcgtcc
     9541 gcttccgttg tgtcgaagtt cgactcgaag gcgagccagt cgctgaacgc gccgtcctgg
     9601 ctcgccggga ccagcaccag ccgcgcgaag tggacgccga gcgcctcgcc ctcgaacgcc
     9661 cggggctgga aggcgcgcgc gccgctgccg ccgaccatgt cttcgcgaat gagcgatagg
     9721 cctctgcgca atgcgggcac atccacgacc cgcgccacca ccgtaagcgt ctcctgccgc
     9781 atccgcccta cgcgtaccaa acccacggct gacgcagcaa gtagcaatca gtttgcggtt
     9841 cggcgcgccg ctccgcggcg gactattttg cgccggctac gcgcacaaag tgcggtcgtc
     9901 ttcgccggtc gagccttcgg tcgagtagcg cgcggccttc aggacttgcg tcgattcgtg
     9961 caaatcgtct gcgtcgacgc gcatcgagac tcgcttcagc ggcagtagca ccgagaagac
    10021 gcttcccgcc ccgggcgtgc tcgtgacggc tacatccccc ccgagtgagc gagccaaccg
    10081 gcgtgtgatg gcgagaccga ggccactacc ttgggcgcga agttcggcgt cgacgtcgag
    10141 ctgatcgaac tcgcgaaaca accggctctg ctgctccggc aaaatcccgg atcccgtatc
    10201 gctcacttca acgcacagcg ttgtttcgtc aatgacgtgg cagctgagcg tcacgcggcc
    10261 cgactcgcca gtaaacttca gcgcgttcga tagatagttg aacacgatct gctgcaatcg
    10321 agtggcgctt tccgccgcga gcgagagctc gcaccgttgg tcgatgagga cgtcgatgtt
    10381 cttctggctt gcggccaacc gcagcgtgct gcgcgccgcg ttcaagatgg cgccaatttc
    10441 gaccggcgcg acggtggcag cgtacgtttc gcgcgcggac tcgagctcag catcgagtag
    10501 gcgcagcatg tggtgcgcgc tgtggatgat gtcgtccatg tactcacgct gaatgaggcc
    10561 catcgggccg gcttgcccgc tgcgaatcag ctctgcaaag ccgatgacgg cggtgagcgg
    10621 agtgcgaagc tcgtgcgaga gttcggcggt tggcaggctc gagccgacgc gtgcggtcac
    10681 ttcactcgag cgaaccctag ccgcgggcgg cgtcgtgctc gcgcgggcca ccggcaccgg
    10741 ctcgctgggg atgctcgccg tctcgaacca gttgggtggc ggctcgagaa cgttgagcag
    10801 cacggtggct cccggctcga ccccggcgac ggtgcaagaa ctgagctcca cgcgcaaatt
    10861 ctgcggtggc gtccagagct cgagggcggc cgcgctcgct tcttcgtcgc gcagtgcgct
    10921 cgccaaaacc ccatcgattg ctgccgggtc ttcgaaccaa tcggcgagcc ggctccccag
    10981 caacgactcg cgcggatggc ccaaaagcag gcaaaacgac gcgctggctt ctacgatggt
    11041 tccgtcagcg tgcgtcgcga ccaggacttc cgtcctcgag tcgatgagcg gcgctccgtt
    11101 cgcgagggag cgagccgcct cggtttcggc gcgctcgacg cggctcaaat cccgcaccac
    11161 cgagatgagg ctcgcggcgc gcccctgcgc gtcacggcgc aaaagcaacg tgagctgtgc
    11221 ccaaaacccc gtgccgtccc ggcgtatttg ataggtccgg ccctcccatt ttccggtgac
    11281 tgccgcgcgc tggaaaatcg aggcagcgcg gccgctcatc acctcgcggg ggtcgtggaa
    11341 catgagcgag ctgcggccta cggcctcgtc cgaggtgtag ccgaaaaggc gctgagcagc
    11401 gtcgttccag ctcgtaatgc ggccgtcgag gtcttgctcg atgaccgcta cgaagcgacg
    11461 tttttcggcc aacgccagca ggacgcggcg ctgagtgtcg aagtcgattg cgcggagcgc
    11521 gcggttcggg ctttgcggaa gcatcaatcc cctcaggttg ttcggcgcac taagcaggtt
    11581 tcgtgccgcg ctcgtgctcg acacgcgacg cagcgtcact tgtttggatg actgcgagcc
    11641 actccggcaa cttttcgagt gagctcgcta cgggtattca aacaccgctc agaagcgcgc
    11701 attgtacggc tttgatccaa attaggtaag acgtgttgaa attacggaga aggtaagagc
    11761 gggtgggcga tacgaatctc ccagacaccg tcggaccgca gtcgaaatcg ttccgcgcaa
    11821 accgcgacga atacgtgacg gatttcgctc gcgtccaaga ttgagaatgg tcgtttcgta
    11881 cggagttcgc gcaggtccct ctagcaagat ccgccgccct ggggctctac taacgcgggg
    11941 acatgccgac catcctcgta gtcgaagaca acccggtgac ccgcaagctg gtgcgtgtgt
    12001 cgctgactgc ggcggggatc gacgtggtgg aggcatcgac gggcagtgaa gccatcgcgc
    12061 tcctgggcga gcgcaacgtc gacttgattc tccaagatct cgcgctcgcg gacatggacg
    12121 gcttcgagtt gctcggaaag ctccgcaagt cgcgcggcag cgagctcccc caggtggttg
    12181 cttttacggg ccttgccgat cgtcaacgcg ttctcgctgc gggtttctcc gacttacttt
    12241 tgaagccgat cgatcccgcg cggctcgttg gctacgtacg cgagcgcttg aacgctggac
    12301 ccactcgaac tcgatcgtct ccgcgcgcgg cgcgcgttgg gtcggacgcg atgttgcaga
    12361 gccctttgtg gagctcggtc tcgaagctcc tcgcgaacct agccgatctc agcggcgggg
    12421 ctgctggctt cgacgacgtg ctgcgcagcg cgctcgcgag cttcctggac tcgtccggct
    12481 ttcaacgtgg cgctgcgtac cttccagccc cgtcggggtc ggtcgcgctc gccgctcagc
    12541 tcggctttca tggcgtgcag ctcgagcggc tgagtgattt cgccgggcgg gtcgcgctgc
    12601 tcgatcgagt gatggtcacg accgaacctc tcgtgatcga ctcggtggtg gtgcccgacg
    12661 acgcgcgcct gctcgcggac ctcgaggcgc attcgctcct catcgtgccg attcttcacg
    12721 gccgcacgcc gaatgccgtt ttcgtcgccg tctccggcaa ccgtggcgtc gcgcccactt
    12781 ggctcgaggt ggggcgcctg atggcggcac cgattggtca gtccctcgcg ctcgcagctt
    12841 cggtcgcgcg gctcgcggcc tccgagcatc gctttcgcgg catcgccgaa tcgacgcgcg
    12901 acggcatcgt ggtcgcggat cccgaaaaca tcgtcactta cgcgaacgct tccgcggccc
    12961 gggtgctctc tcggccgcac gcagagctca tcggcgcgca agtcaaagac atcgtcccct
    13021 tcgtcgccga cggcgcgttc gccggacgtg tgcgccttcc cggtgggcgc gacgtcccgc
    13081 tcgaagtgtc tctgcaagac ttcgaagatc cgcccggtca cttgaaccac gtgtacgtgc
    13141 tgcgcgatct ttcggagcgc ttacgcttgg aagagctcgc ctggctcgcc aaccacgatc
    13201 cgctcaccgg gctcgtgaac cgccgtcgct tcgaagagga gctcaacgcg cgcctcgccg
    13261 aatcgcaacg ctacggcacc ggcggtgcgc tactggcgat cgatctggat caattcaagc
    13321 ccatcaacga tacgcacggg cacgctgcgg gcgacctcgt actgcgagcc atcgccgagg
    13381 tcttgaaatc ggtcacgcgc gcgaccgacc tcgccgcgcg catcggcggt gacgagttcg
    13441 cggtgttgct caaccacacc aacgcctcgg gcgccgacgc ttgcgcgcgg aaactactcg
    13501 agcgcatctc ggagcacgtg acctcgtacg agggcgtcga gctccgcgtc ggtgcgagcg
    13561 tgggggtggc cctcctgccc gaggacggga cgacccgaga ggagctgttc gccgcggccg
    13621 atcgagctct gtacgtatcg aagcagtcag ggcggcggcg ggtcaccttc agcgggatcc
    13681 ctcaattcgg caagccgagc gaggtgccgc ccgaaagccg ctcgtctcgg gaacgcagtg
    13741 tgttgccgcc gcgcggggag cggcgcggct gaagcggtct cttctctgag attgcgctcg
    13801 gttctcggct ataagggcgg catgcgttgg ctgctctcga gctcagtgtt cgccttctcc
    13861 gtgttctcat cgacgctcgg atgcgccgag cgccacgcga gcgtggagag gccgccgagc
    13921 gaatcgtccg tccttcccgc ggctccggct tcggcgacga gcgcggtcgc caccccgagc
    13981 gcttcgacgg ctccgagcgc gcagcccttg cccgcgaaag aagccagcgc ggcggaggtg
    14041 cccgcgccga aagctccggc cgaagccgaa gcagaggccg agccgccgct cacggggccc
    14101 gacggaaaga cgctgccgca aaccgaggag cgtccatcca tcgaatcccc cgcattcaag
    14161 cgccgtatcg agcgcttggc gcagtgcatc ctccgcgacg acccgagcat ctgctcatcg
    14221 agtttctttc cgctgctcgc ttatcgcgag gtcaaagcgg tggagaagcc ggagcgcgac
    14281 tatcagtatc gcctggttcg ccacttcgag cgcgacattc acgagtacca caaagcgctc
    14341 ggcgacaacg ccaagttcga gggcatcgac gtgcccgagg cgagcgctcg ctggatgaag
    14401 cccggctcag agggcaacaa actcggttac tatcgagtgc tgcgctcccg gctgcgcttc
    14461 acgaagagca acggcaaagc acaaaacttc gagcttacgt cgatgatctc gtggcgcggc
    14521 gaatggtacg tagtgcacct gcacggcttc gaatgaacgg cgggcgcgag ccgctcaacc
    14581 cttgccggcg agctgactct tcagctgctt ggccgcgagc gcgtacccac cgttggcgcc
    14641 gtccacgaaa tgaaggtggt taccagcgta ggaccgcacg aagcaggtaa cgagcgccga
    14701 cggcgcgcgg tggagaccgt agaccaggcg cccggcgctg cgttcggcct ccaagaactc
    14761 ttcgagcttt ccaatttcgt cttcggaaag gtcgagcacc atccggagcg tctcgtcgaa
    14821 cttgcgaaaa tcggtgttct cgacgagctc gctcgcgtag tttccaccgt cgaacccgcc
    14881 tgcgctcgcg ccgagcccca tgagcacgcg cccagcgcgc gctttcttct gagcattctg
    14941 gtgagcacga tcgtactcgc gccccgacgt gtctcgcgac aagagccgcg cttcggtcga
    15001 gtagtctccg ccgaacgcct tgagcttgag tcgctccacc gaaacggggc gcgcctcgtc
    15061 ttggccgtga atgcctgcca gcgcgccgag cacggagcga tacacctctg cctgggccgc
    15121 ttcgtcgcga gcgagcgcct gcacgaggag actcacgatc gaaccctgct tgctttcgac
    15181 cggttgccag cgacactcga acccttcgaa gctcgcgttc gcgtcgtcgc tgggcggaat
    15241 cgagtagctc gcggcgcggc tcgaatcctt gatccagcgc tcggccgtcg cgaagccgct
    15301 gcctgcgaac atcgccaagc aaacgtgagg gctcggccgg aagcgcgcca cgcgcacggc
    15361 gtgtccctcg tcgtacagcg ccgccaccgg gaccacgccg gtccgaagct cgagttcgta
    15421 cgctgaaacg gccacttgag ctacgccgcg tagcgctcgt gcgcacgccg ccaggtgagc
    15481 tcccggcacg agcagcgttg ctccgtcccc gccgaacacg tacggcagct gcacgctcgg
    15541 cagcgcattg cacaccgcga cgatcgaggc gaccccgagg gcgttcacgt cgcggtaccg
    15601 acccgcctcg atcgcgcggg tggagccccg cacgtcggtt atcacgatat gccagctagc
    15661 cggtgccgcg tggtacgcag agcgctcggt cacgtgggcg aaatcggtga tctcgggaag
    15721 ccgtgcgaaa aactcgacgt cgtccggctg cggctcgaac gcgacgggag gcatgccgac
    15781 ggaactttac acgccgagca cgcggcggcc aaacgtaacc attgcggccg cgccggggcg
    15841 gctcgcgctt gctgcccgcc gatggtgtag catccccggc atgaaggctt cgcgcggctg
    15901 cgttgcactc ctcactttgc tttgcgccaa aaccgtgttc gcacagtcgc ccgctcctgc
    15961 aacgcctggc gctggtcccg caacgcccgg cgctggtccc gcatcgcccg gcgctggccc
    16021 tgcatcgccc gccacgggtc ccgcaacgcc cggcgctggt cccgcaacgc ccggcgctgg
    16081 ccctgcatcg cccgccacgg gtcctgcatc gcccgccacg ggtcctgcgc cggttagtgc
    16141 cgcgccgccg ggcgagatca tcctcactcc gccgccgagc gcgccttcgg agccacctgc
    16201 cgcgccgccg actgctccgc cggtggagcc gcctccgtct gcgcccccct cgatcgagcc
    16261 gcagctcgaa gtcaaacgac gcgcgaccac tcccatgccc cccgtgtctg gtaagcccca
    16321 cgatttcaac atcgagctcg gcttcggggt gaacggccgc ttcggtcagg cctccggcta
    16381 cagtgacgac gaaggcttgg gggccgcata cggcgctggg ttttggttcg ggctcagcga
    16441 cgcggtcgcg ctcgggctag agcttgctcg aatcgatctc ggtcgtgcga gcgcacacag
    16501 cgggcagaac ttcgtgactg ccgagtacgc gagcaccggc gcttcgctcg gcgggcgctt
    16561 ctttgctttc caaacagagc acctgcgcct gttcgcggct ctgcgtgtcg gcttggcact
    16621 cgagcacgtg tctgcctacg gcgtgcgcgc actcggtact ccgctcgacc cggccacgtc
    16681 cttcaactgc agtggcacga gtgggcccgc gctcggtttc ggcgctggca tcggaggcgt
    16741 gtttcacctg aactcgcatc tcgacttcgt ctcgcgcctc gacggccacg tcgaacagct
    16801 gaccagcgac gtcgtgaacg gttgcgccgc gggcgtagga tcgacggcga gcctccttct
    16861 cggggtaggc ctcgcgtacg gtttcgatgg cccagcgagc agcggggacc catacgctgc
    16921 ccgtcgggga aacaagttct aaacatccgc tgacgcggtg cgggtcgcgg cgcggtggtt
    16981 tgctccgccc aggcgatgga aatcgtaccg cggcgcccga cccctgatta gcccgtgcta
    17041 aattcgccgc gtgagctcga agcccccacg cgacgcaaac tcagagcgcg agcacaatca
    17101 cgctcacgcc gcccacgatc acgctcacgc ggctcacgat caagatcacg ctcacgcggc
    17161 tcacgatcac gctcaagacc acgatcacgc cgctcacgat cacgccgctc acgatcacgc
    17221 cgctcacgat cacgccgctc acggtcacgc cgatcacgcc gctcccggtc acgccgctca
    17281 tgagcacgcc gacagcggcc acgaccacgc cgaccacgag tcctccccgc gtgaagagtc
    17341 ggcgcgcccg gcgcagcgag ctccggtgcg catccgtccc gccggcggac acgggcacgg
    17401 ctcgcatggc catgggggcc acggccacgg aggccatgga catgggggac atgggcactc
    17461 cggcgctgcg ctgcgagcgc tcgtcctcgc gctcgtcgcg actggcacgt tcaggctggt
    17521 cgaggccggc gtcgggcttt ggtccggcag tttggcgctg ctggccgacg ccgggcacat
    17581 gttggctgac gctggcgctt tggccctcgc cctcgccgcg caacgctttg cgactcgtcc
    17641 tcgcacggaa cgctccacgt ttggctatcg ccgcgccgag gtactggctg cgttcgtgaa
    17701 cggcatggcg ctcgcgggag ttgccgttct catcgtgaag gaagcgatcg agcgctgggt
    17761 ctcgcctcac gaaatgaacg gcagagcggt gttgctcacc gcgtttctcg gcctgtgcgt
    17821 gaacctcatc gtcgccgcca tcttgatgcg cgggcaaaaa gacaacatca acctacgcgc
    17881 cgcgttcgcg cacgttttga tggacgccgt tgggtcagtg ggcgcgatgc tcgcgggtct
    17941 cgccgtcacg ttcctcggtt ggtaccgagc ggacccgctg ctctcggcgg gcatcgccgt
    18001 gctggtagcc gtcagcggat ttcgcatttt gaaggaggcc gcctcgatct tgttggagtc
    18061 ggcgccgcct catctcgacg tcgccgagat cgagaagacc atcatgtcgt gtgacggcgt
    18121 ttcggaggta cacgacctcc acgtgtggcg catctccgag cgcttcgaca ccttgaccgc
    18181 tcacgtcgtc ttgaagcgcg gcaagcacgg caccgacgtg tgtcgtcacg tcgccgagca
    18241 actgaagagc gcgtacggcc tcgatcacgt gacgattcag ccggagccgc cgcgtcctga
    18301 cgacctcgtc agcttgcgtc gcagtcgcga tggcggaccg ctgcgcaacg ccggttgaat
    18361 cggcgtgctc ggtggccggg gcgggcgcag tcctggtcgc tttttggggg aggtgggttt
    18421 gggggatgcc gcttttgctc gaagaaattt cgctgtccga cggcgtttcg ctcgaaatgt
    18481 gaggcgttcg gctccgccgc ttgggcggcc caggttggct aggcttccaa gccgcggcct
    18541 ttggagcgcg ggccgatgct cgatcgggtt cgaaccgagg ctcggggacg acggcgtgat
    18601 acgctcctcc gccatgattc aggcgagcca agacttggag acttttctcg gccggctcga
    18661 gcgaccgttc gagcgagccg actccggtac gtacgtgatt cgcatgggcg cggggggagc
    18721 gctcgttgcg ttgcgcatcg aaccgccggt gctggtgatc cgagttgaaa tcgggcgcgt
    18781 cccgagctcg ccgcaggcaa gcctttcact attcaagaaa ctgctcgagc tcaacgcgag
    18841 ctcgatgctg cactcggctt acggcttgga aggcgaccgc atcgtgctcg cggcagcgct
    18901 cgagctctcc agcctcgact tgaacgaact cgaggcggtg ctctccgaca tcgatttggc
    18961 gcttgccgaa cacgtttcga cgctgcacga gttggtcaag agctgatcgg ggttcaccat
    19021 gggaatcttt tcgagactcg cgtcgctgat caaagccaac atcaacgatc tcatcaatcg
    19081 ctcggaagac cccgagaaga tgctcaacca gatcgtgatc gacatgggcg agcagctcat
    19141 cgaggcgcgc aagcaagtcg cctccagcat cgcagacgag aagcggctgg aacgacaggc
    19201 ccaagaagag ggagccaagg ccgccgagtg ggaacgccga gccatgctcg ccgtgcgcgc
    19261 cggcgacgag aacctcgcga aggaagcgct cgctcggaag aaggagcacg agaccctgcg
    19321 ggctcagttc caagagcagt ggcaaaagca gaaccaagcc gtcgagcaac tgaagcttgc
    19381 gttacgcgct ctcaacaaca agatcgaaga ggcgaagcgt aagaagaacc tgctcatcgc
    19441 tcgcaagaag cgggcggagg cgcaaaggtc gatctccgag acgatgagcg gcctcaagaa
    19501 cgcgagcgcg ttcgaagcgt tcgatcggat ggcgagcaac atcgaacgca tggaggcgga
    19561 ggccgaggcc acgtccgagc tgcacgagca gttcaccggg gacaccctcc agcacaagtt
    19621 cagtgagctc gaggccgcgc acggcgtgga cgaagatctc ctggctctga agcggaagat
    19681 gggtttggcc ccgccggaag ctccccccgc cgcggccccg gtgcgcgtcg aggccgcgcc
    19741 gcagggcgtc gatgcggcgg agcaggaaga gctcgcccgt gcgctcgccg agctcgacgc
    19801 ggaacaaaat gaaccacttc gcatgaagag gtaatcacct aagagccccg acgaagggca
    19861 cttcgccgcg ggccgcgcgc ctcgaggacg agccttaccc cagggtaatc actccacttt
    19921 ccccgcggag cttaaccgcc gagcggggcg cgacgtatct ggtctccgct acatttggcg
    19981 ctgcgtgata tagagcgcga cctttgagga ctctcatgcg gcacgtgggt ctaggactga
    20041 gtttcgtcat ggcgctcctc gtcagcgcga ccgacgcggg cgcgacgggc cgcggcttcg
    20101 tcgctgagcc gcgtcaaggt gagaagattc gcatcgacgg cgacttgcgc gagtggcccg
    20161 cgaagatgac gatcctagcg gagtctcgcg agggaagcgc gatcgatgcg cgcgcggtga
    20221 ttggctacga cgacgccaac gtctacttgg cgcttcgcgc ggcggacaag cgcatcgcgc
    20281 gcaccgctca aggcggtgac agtgaagacc acgcgacctt gacgctcgcg ttcccgcgcg
    20341 ggcgcggcga gtactcgatc tatccagtgc agatatatcc gggcagcccc gggcgcctgc
    20401 ctgctttggt caagctcggc ggaaaggccg tgagtggtgc caaagcagtc gagaacgcca
    20461 ccgacaaaga attgttcctc gaggcgcaga tcccgtggac gacgtttccg gaggcggcga
    20521 agctcagggt cgggcttcgc gccgccgtga gctacaccaa cgcggacgct cccggtagcg
    20581 tgaagggcat cg
//
LOCUS       DASTKS010000849         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1922743, whole genome shotgun sequence.
ACCESSION   DASTKS010000849 DASTKS010000000
VERSION     DASTKS010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922743"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="argE"
                     /locus_tag="VFQ35_17610"
     CDS             <1..1198
                     /gene="argE"
                     /locus_tag="VFQ35_17610"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET9932526.1"
                     /translation="VSPTPRCLELTRALVNIDTVSNRSNLALIEYVRELLVRSGVKCR
                     LTYDDERQKANLFATLGEGKPAGVVLSGHTDTVPWDGQDWSFDPLSGRIEAGRLYGRG
                     SADMKGFLAAALAHVDDFLASDSPFAVHLAFSYDEEVGCFGVRRMLKDMRDAKIAPLA
                     CVVGEPTSMVPAVAHKGVYRYKCSVRGKEAHSSLTPRSVNAIEMAARVVSKVRDMAEA
                     LERAEPRYPGFDVPFSTSSVGQFHGGIADNVVPRDAEFRYEFRNLPTTDAARLQAEVV
                     HYARELEAGMRRVAPETGFRFDTICEVPSFLGSEAHPLTRLAQRLSGEARTTLVAFGT
                     EAGLFQAAGVPTVVCGPGSIEQAHQPDEYVSLEQLARCEAFMRSLATSQDLHSLAEAL
                     NATGTI"
     gene            1195..1683
                     /locus_tag="VFQ35_17615"
     CDS             1195..1683
                     /locus_tag="VFQ35_17615"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET9932527.1"
                     /translation="MKPSRALSVSVIALALGVACSKPKPPTVRPLSARVVAVTPSEIR
                     LAVDLEVTNPNGFPLVARSVDGTLSVGSGAELGRSHTDLASSIPAGATANVTSEVSVG
                     FQNLGALAPFVLSPLPVPYELHGSATLGGEKLNVSVPFTLNGELTRAELVAIGLNHLA
                     PR"
     gene            1773..>3175
                     /locus_tag="VFQ35_17620"
     CDS             1773..>3175
                     /locus_tag="VFQ35_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9932528.1"
                     /translation="MSALKVLVVDDQPAVLAALHLLFDVRGIPTLVAKTPEEALELVR
                     TDEIGVVLQDMNFSRDATSGAEGESLLASIRALDPELPVVLMTAFQSLEAAVRLVRAG
                     ASDYIAKPWDDDKLLATVKNLMRLREVSRENLRLRSSGSRARAELQREHDLCGLVYAS
                     AALHEVVALAAKVAPSEAAVLITGPNGSGKELIAQIVQANSRRKKGPFVKVNAGGLPD
                     ALLEAELFGAEAGAYTGASKLRVGRFEEASGGTLFLDELGNLSMMAQMKLLRVLQTGE
                     YQRLGSNVTRKTDVRLISATNADLRAAIRAGTFREDLYFRLNVIELCVPALRDRPDDI
                     LPIAERVLARHEAEGGKRFELSPDARRALVDHDWPGNVRELENRLQRATLVSDGQRIS
                     AAALGLETPLGAAVAPARTAEPVNEPASRPPALPAPDPASSEARDSDPGKEAERAELE
                     RVLVESGGVISTAAAMLG"
BASE COUNT          534 a         1077 c         1044 g          520 t
ORIGIN      
        1 cgtctcaccc acgccgcgtt gcctcgagct cacgcgagct ctcgtgaaca tcgacaccgt
       61 gagcaaccgc tcgaacctcg cgttgataga gtacgttcgc gagctgttgg tgcgctcggg
      121 cgtgaagtgc cggctcacgt acgacgacga acgccagaag gcgaacctct tcgccacgct
      181 gggtgaagga aaaccggccg gagtcgttct ttctgggcac accgacacgg ttccgtggga
      241 cggacaagat tggtctttcg accccctctc gggacgcatc gaggccggcc gactctacgg
      301 gcgaggctcg gccgacatga aggggtttct cgcggctgca ctcgcgcacg tcgatgattt
      361 cctcgccagt gactcgccgt tcgcggtgca cctcgcgttc agctacgacg aagaggtggg
      421 ctgcttcggc gtgcggcgca tgctcaagga catgcgcgac gcgaagatcg cgccgctcgc
      481 gtgcgtcgtg ggcgagccaa cctcgatggt cccggccgta gctcacaaag gcgtctaccg
      541 ttacaaatgc tccgtgcgcg gaaaggaagc tcattcgtcg ctcacgccgc gctcggtgaa
      601 cgccatcgaa atggccgcgc gcgtcgtgtc gaaggtgcgc gacatggccg aagctctcga
      661 gcgtgccgaa cctcgctacc ctgggttcga cgtccccttt tcgaccagca gcgtcgggca
      721 atttcacggc ggaatcgccg acaacgtggt cccccgcgac gccgagttcc gatacgagtt
      781 tcgcaacctc cccaccaccg acgctgcgcg cctgcaagcg gaggtcgtgc actacgcgcg
      841 cgaactcgaa gccgggatgc gacgagtcgc gcccgaaaca ggatttcgtt tcgacacgat
      901 ttgcgaggta ccgagctttc tcggctcgga agcgcacccg ctgacccgcc ttgcgcagcg
      961 gctctcagga gaggcccgca cgacgctcgt cgcctttgga acggaggcgg gcctttttca
     1021 agccgcgggc gtcccgacgg tcgtctgcgg gccgggaagc atcgagcagg cgcatcaacc
     1081 cgacgagtac gtgagcctcg agcagctcgc acgctgcgag gcgttcatgc gctcgctcgc
     1141 gacgagccaa gacctccact cgctcgccga agccctgaat gctacgggga cgatatgaag
     1201 ccatcgcgcg cattgtcggt cagcgtcatc gcgctcgcgc tcggagtcgc ctgcagcaag
     1261 cccaagcccc ccaccgtgcg gccgctcagc gcgcgcgtcg tcgcggtgac gccgagcgag
     1321 atccggctgg cggtcgacct cgaggtcacg aacccgaacg gctttcctct cgtcgcgcgg
     1381 agcgtcgacg ggacgctcag cgtcgggagc ggcgcggagc tcggtcgctc gcacaccgac
     1441 cttgcatcga gcatccccgc cggtgccacc gccaacgtca cgagcgaggt ctcggtcggc
     1501 tttcaaaacc tcggcgcgct cgcgccgttc gtgctctctc cgctgccggt tccgtacgag
     1561 ctccacggga gcgcgacgct cggcggcgag aagctcaacg tgagcgttcc cttcacgttg
     1621 aacggcgagc taactcgtgc tgagctcgtc gcgatcgggc tgaatcacct cgctccgcgc
     1681 taaacttgtc cattcgcggg atctaagcgc gccctccgct ggtcccagcc gtgaagatcg
     1741 gctaacggtg gagctctacg cgagtcacga gcatgagcgc cctcaaggtc ctggtagtcg
     1801 acgatcaacc ggcggtgctg gctgcgctgc atcttctgtt cgacgtgcgt gggattccga
     1861 ccctcgtcgc gaaaacgccc gaagaagcgc tcgagctcgt gcgcaccgat gagattggcg
     1921 tcgtcctgca agacatgaac ttcagccgcg atgcgacgag cggcgcggag ggcgagtcac
     1981 tgctcgcatc cattcgcgcg ctggatccgg agctgccagt cgttttgatg accgcgttcc
     2041 agtctttgga ggcagccgtc cggctcgtgc gcgccggggc gagtgactac atcgcgaagc
     2101 cgtgggacga cgacaagctg ctcgcgaccg tgaagaactt gatgcgcctg cgcgaagtct
     2161 cgcgcgaaaa ccttcgtcta cgatcctcgg ggtcgcgcgc ccgcgcggag ctgcaacgcg
     2221 aacacgacct gtgcggcctc gtttacgcga gcgcagcgct gcacgaggtg gtggcgctcg
     2281 ccgcgaaggt agcaccgtcc gaggcagccg tgctcatcac cgggccgaac ggatccggca
     2341 aggagctcat cgcgcagatc gtgcaagcga actctcgccg caagaagggg cctttcgtca
     2401 aggtcaacgc cggcggcctg ccggatgcgc tgctcgaggc cgagctcttc ggcgccgaag
     2461 cgggtgcgta caccggcgcg agcaagttac gtgtcggccg cttcgaggag gcgagcggcg
     2521 gaacgctgtt cttggacgag ctcggcaacc tctcgatgat ggcgcagatg aagctcctgc
     2581 gagtcttgca aaccggagag taccagcggc tcggcagcaa cgtgacgcgc aagaccgacg
     2641 tgcgcctcat cagcgccacc aacgcagatc tgcgtgccgc cattcgcgcc gggaccttcc
     2701 gtgaagatct gtactttcgc ctgaacgtca tcgagctctg cgtgccggcg ctgcgcgacc
     2761 gccccgacga cattctgccc atcgccgagc gcgtgctcgc gcgccacgaa gccgagggcg
     2821 gaaagcgctt cgagctttcc ccggatgcgc gccgcgcctt ggtcgatcac gactggcccg
     2881 gcaacgtgcg cgagctcgag aatcgcttgc agcgggcgac gctggtgagt gacgggcagc
     2941 gtatcagcgc ggccgccctc gggctcgaga cgccgctcgg tgctgccgtg gcgccggcgc
     3001 gcacagcgga gccagtgaac gaacccgcgt cgcgaccgcc agcgttgcct gcccccgacc
     3061 ctgcctcgag cgaagcccgc gattcggatc cgggcaaaga ggcggagcgc gccgagctcg
     3121 agcgcgtctt ggtcgagagc ggcggcgtga tctcgaccgc cgcggcgatg ctggg
//
LOCUS       DASTKS010000850         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1922910, whole genome shotgun sequence.
ACCESSION   DASTKS010000850 DASTKS010000000
VERSION     DASTKS010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922910"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFQ35_17625"
     CDS             complement(<1..281)
                     /locus_tag="VFQ35_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET9932529.1"
                     /translation="MEFVVREPEGRATPLVVEVPHAGLYVDATALSTLLAPANALGRD
                     ADLYVDELFSDAPNEGAALITARVSRYVVDLNRAENDFDALAVENGSARV"
     gene            416..1168
                     /locus_tag="VFQ35_17630"
     CDS             416..1168
                     /locus_tag="VFQ35_17630"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9932530.1"
                     /translation="MVTSETPLALSSDSLLGGSVRFRQPTAGYRVNIDSLILAEFARV
                     GRSAGVAIDLGAGVGLVSLLLEHYRAARELVLVEREPAIAAIARENLANARAKVTVHE
                     VDVSAPSLPELLGTRADLVVSNPPFFNRTEHRAPRDEQRERARLGEVLPFLLATAELL
                     AGPKARAAFVYPARSLTEFFDAARRARLVPKRLRFVHAFADRPARLALVELRRARPGG
                     LVVQAPLVEWLAPQVPSPELAALSLDSTSGRK"
     gene            complement(1137..1682)
                     /locus_tag="VFQ35_17635"
     CDS             complement(1137..1682)
                     /locus_tag="VFQ35_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9932531.1"
                     /translation="MAAALVHYPVLDRGKEVVTTAITNLDLHDIARSAHTFGLSDYFV
                     VHPIAAQRELAERVRTHWTQGSGARRIPDRSPALSNLRVVAALDDALSALGAETEIWV
                     TSAQEGEKSLSFAAARARLESEGPPVLLVFGTGWGLAPAVLARAAFQLEPVRSPRSDG
                     YNHLSVRAAAAIIFDRLLSRG"
     gene            complement(1688..>2232)
                     /gene="trmD"
                     /locus_tag="VFQ35_17640"
     CDS             complement(1688..>2232)
                     /gene="trmD"
                     /locus_tag="VFQ35_17640"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9932532.1"
                     /translation="MRPDCVMEGIEATERALGVETPARRVLLTPQGARFDQSAARRMA
                     SYESMVLVCGRYEGFDERVRYFVDEEVSIGDFVLTGGEIPAMCVVEAVVRLLPGVLGN
                     AESTSEESFSPVCGGLLEYPQYTRPASFRGHEVPKVLVSGDHAKIAEWRKEQSRLRTA
                     ERRPDLLGDAARDEGKPRRS"
BASE COUNT          339 a          786 c          746 g          361 t
ORIGIN      
        1 actcgcgcgc ttccgttttc gacggcgagc gcgtcgaagt cgttttcggc gcggttcaga
       61 tcgacgacgt agcgactgac gcgagcggtg atgagtgccg ctccctcgtt cggggcgtcg
      121 ctgaacagct cgtcgacgta gagatccgcg tcccgaccga gggcgttcgc aggcgccagc
      181 agcgtggaga gcgccgtcgc gtcgacatag agccctgcat ggggcacctc gaccacgagc
      241 ggcgttgccc gaccttcggg ctcacgaacg acgaactcca cgagaatcgt gtagcacaga
      301 gccggcggct cgcgggtttc gaggtacttt cgggtcggtg ctgcgccttt ccgcgcagaa
      361 acgcgggtgg ttcgcgtatc ctggtgggag gcagcggccc atccacgcgc tcgccatggt
      421 gacgtcagaa acgccccttg cgctcagctc cgattcgctt ttgggcggga gtgtccgctt
      481 tcggcagcca accgcggggt accgcgtgaa catcgactcg ctcatcctcg ccgagtttgc
      541 gcgcgtcgga cgaagcgccg gcgtcgccat cgatctcggc gcgggcgttg gcctcgtctc
      601 gctgctcttg gagcactacc gcgccgcgcg cgagctcgtc ctcgtcgagc gcgagcccgc
      661 gatcgcggcg atcgctcgag agaacctcgc gaacgcgcgc gccaaagtca cggtgcacga
      721 ggtcgacgtg agcgcgccga gcttgcccga gctccttgga acgcgcgcag atctcgtggt
      781 cagcaatccc ccgttcttca accgaaccga gcaccgcgcc ccgcgtgacg aacagcgcga
      841 gcgggctcgg ctcggcgagg tgttgccctt cttgctcgcg acggctgagc tcctcgccgg
      901 ccccaaagcg cgcgccgcat tcgtgtatcc cgcgcgcagc ctcaccgagt tcttcgacgc
      961 cgctcgccgc gctcgcctcg tgccgaagcg gctccggttc gttcacgcat tcgcggatcg
     1021 ccccgcgcgg ctcgccctcg tggagcttcg ccgcgcgcgt cctggcggct tggtcgtgca
     1081 agcgccgctc gtcgagtggc tcgctcccca agtgccgagc cccgagcttg cggcgctcag
     1141 cctcgactca acaagcggtc gaaaatgatc gcggccgcgg cgcgcacgct caggtggttg
     1201 taaccgtcgg agcgcggtga gcgcacgggc tcgagctgaa atgcagcgcg tgccagcacc
     1261 gccggggcga gtccccaccc cgttccgaaa acgagcagca ccggcggtcc ttccgactcg
     1321 agccttgcgc gcgcggccgc gaaggacagc gacttctccc cctcctgcgc gctggtcacc
     1381 caaatctcgg tttcggcgcc aagcgcggaa agggcgtcgt cgagcgcggc cacgacgcgc
     1441 aggttcgaga gcgccggtga gcgatccggt atgcggcgag caccggagcc ctgcgtccaa
     1501 tgcgtgcgaa cgcgctcggc gagttcgcgc tgcgcggcga tcgggtgcac gacgaagtag
     1561 tcgctgagcc cgaaggtgtg cgcgctccga gcgatgtcgt gaaggtccaa attggtgatg
     1621 gccgtcgtga cgacctcttt cccgcggtcg agcaccgggt agtgcacgag cgcggcggcc
     1681 acgcgcctca cgagcgcctc ggctttccct cgtcccgcgc cgcatcgccg agcagatcgg
     1741 ggcggcgctc ggcggtgcgc aggcgcgact gctccttgcg ccactccgcg attttcgcgt
     1801 gatctccgga caccaacacc ttgggtacct cgtggcctcg aaagctcgct gggcgggtgt
     1861 actgcgggta ctcgagcaaa ccaccgcaga ccgggctgaa agactcttcg ctggtcgact
     1921 cggcgttgcc gagcaccccg ggcaggagcc gcacgaccgc ctcgacgacg cacatcgcgg
     1981 ggatttcacc gccggtcagc acgaagtcgc cgatcgagac ctcttcgtcg acgaagtagc
     2041 gcacgcgctc gtcgaagcct tcgtagcgcc cgcacacgag caccatcgac tcgtaagagg
     2101 ccatgcgccg agccgcagat tgatcgaagc gagcgccctg cggagtcagc agcactcggc
     2161 gggctggcgt ttcgacgccg agcgcgcgct cggtagcctc gatgccctcc atcacgcagt
     2221 cgggacgcat ca
//
LOCUS       DASTKS010000851         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1027583, whole genome shotgun sequence.
ACCESSION   DASTKS010000851 DASTKS010000000
VERSION     DASTKS010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027583"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /gene="brxE"
                     /locus_tag="VFQ35_17645"
     CDS             <1..707
                     /gene="brxE"
                     /locus_tag="VFQ35_17645"
                     /inference="COORDINATES: protein motif:HMM:NF033448.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-6 system BrxE protein"
                     /protein_id="HET9932533.1"
                     /translation="HAARVCAAFGQSASALLPLFGASRLPPGGTLAAQCSARLRLERT
                     HGLPLTPQQLDEVLALQLSVAWAGEAAGAPRRLGWWETDLIDAEGGGDLFARLVPRTA
                     AWAGFELARAAARRVEFAALSRMARRDAVWTLFHFGFELDEQLDERLAHHRRHRHEPA
                     QVLGPGLRAGGGWSAEDFAAWLKSHGDAPSELTPSGRLIHAKPADATQAARLLASALV
                     PFERHYPLPFLEVGGG"
     gene            704..853
                     /locus_tag="VFQ35_17650"
     CDS             704..853
                     /locus_tag="VFQ35_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932534.1"
                     /translation="MTARPAEASELHTRILRCMLAADDCYAYWQHVDVSVPVEARAAV
                     AFRER"
     gene            945..2810
                     /gene="pglZ"
                     /locus_tag="VFQ35_17655"
     CDS             945..2810
                     /gene="pglZ"
                     /locus_tag="VFQ35_17655"
                     /inference="COORDINATES: protein motif:HMM:NF033443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-6 system phosphatase PglZ"
                     /protein_id="HET9932535.1"
                     /translation="MCAAAAGLAIAKHPLPLKGCASLEEVLDRYTGELAEVDRLHRTF
                     EQRAHALITPELEDYDQLLEARGVLRATYRDWADGLGRAFAALCRTHGALPPAELRQR
                     QIFGRSVKPLVEQGGRVAFFMVDALRFEMAQALSEELKRDKFSVALKACFAELPTITS
                     VGMNALAPVERQGRLELAFDGDKLSGLRAQGYAVKTPAERLRAMREATGSALDFELET
                     LSAMTTAELKRALGTKPGLVVVRSRELDDAGEVGLHLGTFDHTLTLLRSAVSLLQQAG
                     VERFVIASDHGFLLQDSGAQKHPYGSNKRVADRRHVLATQQSGMPDVLEVPLAALEYD
                     APTPQFLVFRPDTALWDVASKVAPFVHGGNSLQERVIPVLFVERHGRRGKTTTKYEIV
                     ATPEVGKLGRQRLRLSVRLQKESSAALSFHAPKAIALALRVSERLDVQLTLLDVTPPA
                     KLADGRILLPPNGESALVEFELEGEADEKLRVEVFHPDAVEDVEPKMVEGFFDVFRNR
                     RLPKPVGQTPAPAPTRTEPPPSAPRPPSVPPSLAPRTSAAGDWQSLIEDQGFREVLLY
                     IEERRSMNEVELGQKLGSSRRVRAFTRAFDALMARVPFAVEIMFVSGLKTYVRKD"
     gene            2814..3782
                     /locus_tag="VFQ35_17660"
     CDS             2814..3782
                     /locus_tag="VFQ35_17660"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HET9932536.1"
                     /translation="MPSRYASHVFSRLLNGTVPERGLDEIAVGVEKHRKELQRMLDEA
                     RRGSGDVKFLRGGYGCGKTFMATLAVQDAQEAKFATSFVVVSDNDLHFHKFDELYRKV
                     VGGLSTPACPQGALGDILDRWIGSLEEGLVELGVSEDDPDFDEKVLAKLDEQLAGLTQ
                     GRAPADMTRAVFDDRRGVKGLEALYDRIKFESFSGVASLRQPQLDLKPFDRDRLIRVA
                     LKVRSLHSDLAPEQAEARIPRAFIEQLADKVTEGFRGDVGVVPRQFLRTFANIVDIIA
                     SDPEQDPFKLLQFTAKDLSPEEKAAVEGRPLETPPDDEFGGGTVEM"
BASE COUNT          673 a         1498 c         1414 g          671 t
ORIGIN      
        1 ttcatgccgc acgggtgtgc gcggcgtttg gacaatcggc ttccgccttg ctcccgcttt
       61 tcggggccag ccgcctgcct ccgggtggta ccctcgccgc ccagtgctcg gcgcgcctcc
      121 ggctcgagcg aacgcatggc ctccccctca ccccgcagca gctcgacgag gtactagcgc
      181 tccagctctc cgtcgcctgg gcgggcgaag ccgctggtgc gccccgccgg ctcggatggt
      241 gggaaaccga tctgattgac gctgaagggg gcggtgacct ttttgcacga ctcgttcctc
      301 ggaccgcggc gtgggcaggg ttcgagctcg cgcgagcagc ggcgaggcgc gtggaattcg
      361 ccgcgctctc gcgcatggcg cggcgcgacg ctgtctggac gctgtttcac ttcggcttcg
      421 agctcgacga gcagcttgac gagcgcctgg cgcaccaccg ccggcaccgg cacgagccgg
      481 cccaggtgct cggccctggc ctccgcgcgg gcggcggctg gagcgcggag gatttcgccg
      541 catggctcaa gtcgcacggc gacgcgccga gcgagctcac gccgagtggg cgcctgattc
      601 acgcgaagcc tgccgacgcg acgcaggcgg cccgcctttt ggcaagcgcc ctcgtaccct
      661 tcgagcgcca ctatccgctg ccgtttttgg aggtgggggg cggatgacgg cgcgccccgc
      721 agaagcgagc gagctgcaca cccgcatctt gcgctgcatg ctcgcggcgg acgactgcta
      781 cgcgtactgg cagcacgtcg acgtttcggt gcccgtcgaa gcgcgggcgg ccgtcgcctt
      841 tcgagagcgt tgaagagcga ggcgcgcttc gtggatggcc tcgccgagac ggctggaaaa
      901 cctgacgccg cgttctccta cccagtggtg cgctttcgcg attcctgtgc gccgcggccg
      961 cgggcctggc catcgcgaag cacccgctgc cgctcaaggg ctgcgcatcg ctcgaagagg
     1021 tgctcgaccg ctacaccgga gagctcgcgg aggtggaccg gttgcaccgc accttcgaac
     1081 agcgcgcgca cgccctcatc acgcccgagc tcgaagacta cgaccagctc ctcgaggcgc
     1141 gcggcgtcct ccgcgccacg taccgggact gggccgacgg cctggggcgc gcgttcgccg
     1201 cgctctgtcg cactcacggc gccttgccgc ctgcggagct caggcagcgg cagatcttcg
     1261 gccgcagcgt aaagccgctc gtcgagcagg gcggcagagt cgccttcttc atggtcgacg
     1321 cgctccgctt cgagatggcg caagccctca gcgaggagct caagcgcgac aagttctcgg
     1381 tcgccctcaa ggcttgcttc gccgagctac cgacgatcac gagcgtcggc atgaacgcgc
     1441 tcgcgccggt cgagcgccag ggccggctcg agctcgcctt cgacggcgac aagctcagcg
     1501 gcctgcgcgc gcagggctac gccgtgaaga cgccggcgga gcggttgcgc gcgatgcgcg
     1561 aagcgacggg cagcgccctc gacttcgagc tcgagacgct ctcggccatg accacggcgg
     1621 agctcaagcg agcgctcggc accaagcccg gcctcgtcgt cgtgcgcagt cgcgagctcg
     1681 acgatgccgg cgaagtgggg ctgcacctcg gcacgttcga tcacacgctc acgctcttgc
     1741 gctcggcggt gtcactcttg cagcaggccg gcgtcgagcg cttcgtcatc gcttccgacc
     1801 acggcttctt gcttcaggac agcggcgctc agaagcatcc gtacggcagt aacaaacgcg
     1861 tggccgaccg gcggcacgtc ctcgcgactc agcagtccgg catgcccgac gtgctcgagg
     1921 tgccgctcgc ggccctggag tacgacgcgc cgacgccgca gttcttggtt tttcgtcccg
     1981 acacggcgct ctgggacgtg gcttccaagg tcgcgccgtt cgtgcacggc ggtaatagcc
     2041 tccaggagcg cgtgatcccg gtgctcttcg tggagcggca cggccgccgc ggcaagacga
     2101 cgacgaagta cgaaatcgta gccacgccgg aggtggggaa gctcgggcgg cagcgactca
     2161 ggctcagcgt tcggctgcag aaggagagct cggccgcctt gagcttccac gcgccgaaag
     2221 cgatcgcgct cgccctgcgc gtgtccgagc gactcgacgt gcagctcacg ttgctcgacg
     2281 tgacgccgcc cgcgaagctc gccgacggtc gcattctttt gccgccgaac ggcgagtctg
     2341 cgctcgtcga attcgagctc gagggcgagg ccgacgagaa gctccgcgtc gaggtgtttc
     2401 acccggacgc ggtcgaagac gtcgagccca agatggtcga gggctttttc gacgtgttcc
     2461 gcaatcggcg gctgccgaaa ccggtaggac agacgccagc gccggcgccg acgcgcaccg
     2521 agccgccgcc cagcgcgccg cgcccgccct ccgttccgcc ttcgctcgca ccccggacca
     2581 gcgccgcggg ggattggcag agcctgatcg aagaccaagg cttccgcgaa gtgttgctgt
     2641 acatcgagga gcgccgcagc atgaacgagg tcgagctcgg ccagaagctc ggctcctcgc
     2701 gccgcgtgcg ggccttcacc cgcgcgttcg acgcgctgat ggcgcgggtg cccttcgccg
     2761 tcgaaatcat gttcgtgtcg ggcctcaaga cctacgttcg gaaggattga acgatgccgt
     2821 cgcgctatgc ctcccacgtc ttcagtcgcc tgctgaacgg cacggtgccc gagcgaggcc
     2881 tcgacgaaat cgccgtcggc gtcgagaagc accggaagga gctccagcgc atgctcgacg
     2941 aggcccggcg ggggagcgga gacgtgaagt ttctgcgcgg cggctacggc tgcggcaaga
     3001 ccttcatggc gaccttggcc gttcaagacg cgcaggaggc gaagtttgcc acgagcttcg
     3061 tcgtcgtctc cgacaacgat ctgcactttc acaaattcga cgagctctac cgcaaggtag
     3121 tcggcggcct cagcaccccg gcgtgcccgc agggcgcgct cggtgacatt ctggatcgct
     3181 ggatcggctc gctcgaagag ggcctcgtcg agctcggcgt gagcgaggac gatcccgact
     3241 tcgacgagaa ggtgctcgcc aagctcgacg aacagctcgc gggactcacc caaggccgcg
     3301 cccccgcgga catgacgcgc gcggtcttcg acgaccggcg tggcgtgaag ggcctcgagg
     3361 cgctctacga tcgcatcaag ttcgagagct tcagcggcgt cgcgagcctc cgccaacccc
     3421 agctcgatct caaacccttc gaccgcgacc gcctgatcag ggtcgccctc aaagtgcgct
     3481 ccctgcactc ggacctcgcg cccgagcaag ccgaagctcg catccctcgc gccttcatcg
     3541 agcagctcgc cgacaaagtg accgaaggct tccgcggcga cgtcggcgtc gtcccgcgcc
     3601 agttcctccg taccttcgcc aacatcgtcg acatcatcgc cagcgacccc gagcaggacc
     3661 ccttcaagct cctccagttc accgccaagg acctctcccc cgaggagaag gccgccgtcg
     3721 aaggccgccc actcgagacc ccaccggacg acgagttcgg cgggggcacg gtcgagatgt
     3781 gaggtggaat tcagcctaat ttatggtgcg tgaggccgag ggtgagctaa tgttcaggga
     3841 atagggcgcg gacgctggcg cgtccttcac agcgacccat ggcctggaac cccctccaag
     3901 cgtacacttg aacagtacac atgtcgcgga acatccgcct ggtcgccgcg cgcaaggctc
     3961 tcggtcgaat cgtcgaagaa agtcgaacgg accggaactc ccgtgagcct gacgcgccgg
     4021 ggaaaaatcg tcgctcgcat cgtgcctgag gaccgccggc gcgcgcgcga cccgcttgcg
     4081 tccagccgcc cgacccgcag cacgggacgc tcgagctgcg cggcagcgcc gagctcgttg
     4141 gccccgattt cgcgtttgag cttcgttttg gctgcgctcc gctgctcacg tacttcagta
     4201 cgctccgctc cggtgctcgc caaagcctcg ccgaaacgcg aacttggcga gcgggc
//
LOCUS       DASTKS010000852         5934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1027953, whole genome shotgun sequence.
ACCESSION   DASTKS010000852 DASTKS010000000
VERSION     DASTKS010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027953"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFQ35_17665"
     CDS             complement(<1..563)
                     /locus_tag="VFQ35_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9932537.1"
                     /translation="MKIVIVGAGIGGLALGVALRLRGHEVQILERRADFSDEGAGVVL
                     GPNVMAAVRLLGLDAAIAEQGRAVTAMNIADQHGRVLARSAYRVTALPMPAIAIHRSR
                     LHRALLERFDGRLELGVQPSQVILGERPSLRLQEREVECDLIVGADGIRSQVRAALAP
                     DFATRYSGATCFRFVVDEQWTDEAFEQ"
     gene            799..1176
                     /locus_tag="VFQ35_17670"
     CDS             799..1176
                     /locus_tag="VFQ35_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932538.1"
                     /translation="MLLGRTPLEPRQFLDHDARERGLSRIRKQQGRKQAESAVDQRLA
                     PRILQSALENRDGVFAPDFVEEALLGLRIEVNGAEPDPRLVGDASERRGLETIFAELT
                     PGDLENPLTFRGLVEFTQRAHKN"
     gene            1455..2744
                     /locus_tag="VFQ35_17675"
     CDS             1455..2744
                     /locus_tag="VFQ35_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9932539.1"
                     /translation="MRLFKQGSACAALLAALSGVGGTSGCSGGSGSEQAAPPWQGSTA
                     RGGARNASGGASPIMIPSSGGTAAGGASSSSPSTGGAPSIGLGGSSGSAAQGGEKACS
                     AETREGRRTPLDMYFLVDSSGSMADDVEGGTKWELVSSALIDFLDSPRNSDLGAGIGY
                     FPNDAPTDCSSPQSDCVCDPFFGLCFPAEGGSCEVADYETPAVPLAIPTRRGDVVTDL
                     WSHDVVGSTPTRPALEGALKYLSTWAAQHADRKSVVVLATDGEPTECDANTPDDVADI
                     AARALAANPAIQTFVIGVGDSLESLDVIARAGGTGHAYLVDPRANLSSAFAEALDKIR
                     GAAGSCDFTIPSASSSGEKVNPSKVNVRLTVNGVPTRISQTFGNDPSTCGDQGGWYFD
                     NPSAPTRIRLCESTCQALSGGTIDVEFGCDTVVQPPR"
     gene            complement(2758..3015)
                     /locus_tag="VFQ35_17680"
     CDS             complement(2758..3015)
                     /locus_tag="VFQ35_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932540.1"
                     /translation="MAATWECIEFKMGNDELVVWLNGTQSNAISTTNWAKVGDGANGA
                     ASRRRTGRRLTSRSASVGSSTPAPRFGFDDVALGYSRIGCQ"
     gene            complement(3056..3733)
                     /locus_tag="VFQ35_17685"
     CDS             complement(3056..3733)
                     /locus_tag="VFQ35_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932541.1"
                     /translation="MRTTTAAILGWCVALAAACSGEQDEAAKPPSSPGPNASGGQPNE
                     PGEPIPPTCAGFVRRLTACGVISGTHFARCRDDAPLVPCLVACASAASCEELTNAYCV
                     LAQNEFASCATRCRESLMDFTCKDGSVIKATWKCDGTPDCPDGDDEDCPEGTFVCQGG
                     QRIPLGWRCDNTDDCPGAEDERDCSGSPMFICGNGVAVSESTECDGASDCPGGEDEAD
                     CTKLICN"
     gene            complement(3906..5474)
                     /locus_tag="VFQ35_17690"
     CDS             complement(3906..5474)
                     /locus_tag="VFQ35_17690"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HET9932542.1"
                     /translation="MNDEAPTTRKRWGVPIAVLSSAAFVIFWGFYAIDYFASRIPAAP
                     PSGPVEHYLRFDPGSITDAVSALAGMMAAVFGIVVTVISIIVQLSANRYAGVARMFLR
                     DRLNLAIMGFYVIACVCGIWLSVGIQHDFVPRATLLGMLCATSAGLVLMAPYFGYVFW
                     FVEPMNIIARIRKEAVGTAARGSQSEGVLDLMRAQARTLSGMEELTDITSNSISGKDK
                     IIASGAVDALKDFALDYISRKPKAVPSWFEIGAAIRENPDFVAMDPESLSDLEQRRTW
                     VEWKVMRQYLGIYNEALASMRDINYLIAIDTRYIGEAAATSGDVELLLLVYRFLNSYL
                     RSTLNAREVRTAYNVLNQYRLLIEALIKLERHEHAIEGVRHLLYYGSVSFDMKLTFVT
                     ETVAYDVSALCQVAHEAKSSAEEAMLALFLELDRPLRVSSQEGALLGVRKAQVKLAAY
                     YLTQNEERKARLIADDMANEPRERLRSIRRALERVNTKDFWEIIDRGRNFEYMPPPQR
                     AMLTPFFAWLGVEE"
     gene            complement(5582..>5934)
                     /locus_tag="VFQ35_17695"
     CDS             complement(5582..>5934)
                     /locus_tag="VFQ35_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932543.1"
                     /translation="ARAFTDLPRDLATAADAAAGELGVLEAARGAIAEVGAGDLRRRV
                     HTWFDGLRTLRFLHTVGDHGFPKVPCRDAVSSAAFLAIPGEVSLEAAVVAAREREAAL
                     PPDTGPVRHRLPRS"
BASE COUNT         1079 a         1962 c         1885 g         1008 t
ORIGIN      
        1 cactgctcga aggcttcgtc ggtccactgc tcatcgacga cgaaacggaa gcaagtggcg
       61 ccgctatagc gcgtcgcaaa gtctggggcg agcgccgcac gcacttgcga acgaatgccg
      121 tccgctccga cgatgaggtc gcactcgacc tctcgctcct gcaatcggag cgaaggccgc
      181 tctccgagaa tcacctggct cggttgcacg ccgagctcca accgaccgtc gaagcgctcg
      241 agcaacgcgc ggtgcaaccg gcttctgtgg atcgcgatgg cgggcattgg cagcgcggtc
      301 acgcgatacg ccgagcgcgc gagcacccgt ccgtgttggt ctgcgatgtt catcgccgtc
      361 acggcgcgcc cctgctctgc gatcgctgcg tcgagcccga gcagccgcac cgctgccatc
      421 acgttcgggc cgagcacgac gcctgcccct tcgtccgaaa agtcagcgcg cctttcgagg
      481 atttgcactt cgtgcccgcg caggcgaagc gccactccga gcgcgagccc gccgattccc
      541 gctcccacga ttacgatctt cattttttgc ctcccaacca tcgcaaacga gcctgaggcg
      601 ggcccccagg tggctcagca cggcttcgat gtcgcgcgtc cctgacggca ccacgagttc
      661 gagacaagac gcatagtaaa tagaaaacag cacgctcgcc gcgagcatgg gctccacgcg
      721 ctgggcgagc tcaccgcgag cctgcccgcc gctcaccagc cctgcgagcg ccatccacag
      781 ccgctgtaga tccggcctat gctgctcggc cgcacgcccc tcgagccacg gcaattcctt
      841 gatcacgatg cgcgcgagcg cgggctctcg cgaatacgca agcaacaagg gcgtaaacaa
      901 gctgagagcg ccgtcgacca gcggcttgcc ccccgaatcc ttcaaagcgc gctcgagaac
      961 cgggacggtg tcttcgcgcc agatttcgtg gaggaggccc tgcttggact ccgcatagag
     1021 gtgaacggtg ccgagcccga cccccgcctc gttggcgatg cttcggaacg tcgtggcctc
     1081 gaaaccattt tcgccgaact gacgccgggc gacctcgaaa atccgcttac gtttcgaggc
     1141 cttgttgagt tcacgcagcg agctcataaa aactgaacat gttcaatata gaacatgttc
     1201 gattttctcg caagcccgcg tcgcggcggt gtcgatgtcg cggggcggct cggcagcgaa
     1261 cgacttgcga cgcgtccggc gcatgcggcc gcgctgattg cgacgaggcg ctcacccgat
     1321 ccgcgcgaga cgcacacagt agcgtcaggc acccaacagc gcagcaagcg cctgctcgtg
     1381 actcgacgtc gcgcggcgcg acgcttgcgt tcggcggcgg gtgcgttcgg ggcaaacacg
     1441 cccgcggagg ctacatgcgt cttttcaaac agggatcggc ttgtgcggcg ttgctggccg
     1501 cattgagtgg cgtcggcggt acgagcggct gctctggggg ctcgggttcg gagcaagccg
     1561 cgcccccgtg gcagggctcg accgcgcgcg gcggcgcgcg aaacgcgagc ggaggagcca
     1621 gcccgatcat gatcccgagc tccggcggca ccgctgcggg cggtgcgtcc agctcgtcac
     1681 cgagtacggg cggagcgcct tcaattggcc tggggggaag ctcgggcagc gccgcccaag
     1741 gcggagagaa agcgtgcagc gcggagacgc gcgaaggacg gcggacaccc ctcgacatgt
     1801 acttcctcgt cgactcgtcg ggctccatgg cagacgatgt cgaaggcggc accaagtggg
     1861 agctcgtctc gagcgcgctc atcgactttt tggacagccc tcggaatagc gatttgggag
     1921 ccgggatcgg ctacttcccc aacgacgcgc caaccgactg ctcttcgcct cagtccgatt
     1981 gcgtgtgcga tccgttcttc ggattgtgtt tccctgccga aggtggatcc tgcgaggtcg
     2041 ccgattacga gacgccggcg gtaccgctcg cgatccccac gcgacgcggc gacgtggtca
     2101 ccgacctctg gtcgcacgac gtagtcggca gtacgccgac ccgtcccgcg ctcgagggag
     2161 cgctgaagta cttgagtacg tgggcagccc aacacgccga tcgaaagtcg gtggtggttc
     2221 tggcaaccga cggcgaaccg accgagtgcg acgcgaacac ccccgacgac gtagctgaca
     2281 tcgccgctcg agcgctcgcc gcgaacccgg ccattcaaac gttcgtcatc ggagtgggcg
     2341 actcactcga aagcctcgac gtgatcgcgc gcgctggcgg caccgggcat gcctacttgg
     2401 tcgatccccg agccaacctc tcgagcgcct tcgccgaggc cctcgacaag atccgcggcg
     2461 cggcgggctc gtgcgatttc acgattccga gcgcgagtag ctccggcgaa aaggtcaatc
     2521 ccagtaaggt caacgtgcgc ctcaccgtga acggcgtccc cacccgcatc tcacagacct
     2581 tcggcaacga ccccagtacc tgcggagacc aagggggatg gtacttcgac aacccctcgg
     2641 cgccgactcg catccgtttg tgcgagagca cctgccaggc gttgagcggc ggcacgatcg
     2701 acgtcgagtt tggctgcgac accgtcgtgc agccgccacg ctgaagcgct gcgactgcta
     2761 ctggcacccg atgcgcgaat acccgagcgc cacgtcgtcg aaaccaaatc tcggtgccgg
     2821 cgttgagctc ccaaccgatg cggaacgact tgtaagtcgg cgaccagttc gtcttcggct
     2881 cgccgcccca ttcgcgccgt cgcccacctt ggcccagttg gtggtcgaaa tcgcgttgct
     2941 ctgagtcccg ttcagccaca cgacgagctc gtcgttgccc atcttgaact cgatgcactc
     3001 ccaggtggcg gccatgagct tcgcgctcgg atccctgagt cacgtcagaa ggggctcagt
     3061 tacagattag cttggtgcaa tcggcttcgt cctccccgcc tggacaatcc gaggccccgt
     3121 cgcactcggt gctttcggag acggcgacgc cgttcccaca gatgaacatg ggagagccag
     3181 aacagtcgcg ctcgtcctcg gcgccggggc agtcgtcggt gttgtcgcaa cgccaaccca
     3241 acggaatgcg ctggcctcct tggcaaacga aggtgccctc cggacagtcc tcgtcgtcgc
     3301 cgtccgggca atctggcgtc ccatcgcact tccacgtcgc tttgatgacc gagccgtcct
     3361 tgcaagtgaa gtccatcaag gattcgcggc atctcgtggc gcacgacgca aactcgttct
     3421 gagcgagaac gcagtaagcg ttcgtcagct cctcgcagct cgcggcgctg gcacaggcaa
     3481 ccaagcaagg cacgagcgga gcgtcgtctc ggcagcgagc aaagtgcgtc cccgagatca
     3541 cgccgcacgc cgtgagccgc cgaacgaacc ccgcgcaggt cggcggtatt ggctctcctg
     3601 gctcattggg ctgtccaccc gaagcgttcg gacctggcga gctcggcggc tttgcggctt
     3661 cgtcctgttc gcctgagcaa gcggcagcca aagccacgca ccaccccaag atcgctgctg
     3721 tcgtcgtccg catccggaaa cctccgcgag ccgcgccgtc gcttcggctc cgtgtacgct
     3781 agccgaatcg tcgctgagac gcctaccgcc ctggatccaa agcgggagct ctatcgaagc
     3841 ggaggtcgct gagcgaggaa atttacgccg tactccgagt ggacgtgcgg tggaaccgct
     3901 gggccctatt cctccacgcc gagccaggcg aagaaggggg tcaacatggc gcgctgcggc
     3961 ggcggcatgt actcgaagtt tcgccctcga tcgatgattt cccagaagtc cttggtgttc
     4021 acgcgctcga gcgctctgcg tatcgatcgc aggcgctcac gtggctcgtt tgccatgtcg
     4081 tcggcgatga gacgcgcttt gcgctcttcg ttctgggtga ggtaataggc ggcgagcttc
     4141 acctgggcct tgcgcacccc gagcaacgcg ccttcctgac tgctgacgcg aaggggccgg
     4201 tcgagctcga gaaacagcgc gagcatggcc tcttccgcgg aggacttcgc ctcgtgcgcg
     4261 acttggcaga gcgcagagac atcgtaggcg acggtctcgg tcacgaacgt gagcttcatg
     4321 tcgaagctga cgctaccgta gtagagtagg tgccgaacgc cctcgatggc gtgctcgtga
     4381 cgctcgagct tgatcagcgc ttcgatcagc aagcgatact ggttcaggac gttgtacgcg
     4441 gtgcgaactt cgcgcgcgtt cagagtggag cgcaggtacg agttcaaaaa gcgatagacg
     4501 agtaagagga gctccacgtc cccggaggtc gcggccgcct ccccgatgta gcgagtgtcg
     4561 atcgcgatga ggtagttgat gtctcgcatg ctggcgagcg cctcgttgta aatgccgagg
     4621 tactgccgca tcaccttcca ttcgacccag gtgcgtcgct gctcgagatc cgagagcgac
     4681 tcgggatcca tcgcgacgaa atccgggttt tcgcggatgg cagcgccgat ttcgaaccag
     4741 ctcggcaccg ccttgggctt tcgggagatg taatcgagag caaagtcttt gagggcgtcg
     4801 acggcgccac tcgcgatgat cttgtccttg ccggagatcg agttgctcgt gatgtcggtg
     4861 agctcctcca tcccgctcag cgtgcgtgcc tgagcgcgca tgaggtcgag gaccccttcg
     4921 ctctgcgagc cgcgcgccgc cgtgccgacg gcctctttgc ggatccgggc gatgatgttc
     4981 atcggctcca cgaaccagaa tacgtagccg aagtagggcg ccatcaagac gaggcccgcc
     5041 gaagtcgcgc acagcatgcc gaggagggtc gcgcgcggaa cgaaatcgtg ttggatcccc
     5101 acgctgagcc aaatgccgca cacgcatgcg atcacgtaaa accccatgat ggcgaggttc
     5161 agacgatcgc ggaggaacat ccttgcaacg ccggcgtacc ggttcgcaga gagctgcacg
     5221 atgatgctga tcaccgtgac cacgatgccg aagacagccg ccatcatgcc ggcgagcgcg
     5281 ctcactgcat cggtgatgct tcccggatcg aagcgcagat agtgctctac cgggccgctc
     5341 ggcggcgcgg cggggatgcg cgaagcgaag tagtcgatcg cgtaaaatcc ccagaagatg
     5401 acgaacgccg ccgacgacaa gacggcaatc ggcacgcccc agcgcttgcg tgtggtcgga
     5461 gcttcgtcgt tcatgacatc gtgaggccgg gcccgaagcg gcttcccaca gatatcagag
     5521 aagtcggggg cgcgtcgcgc gaggcttggt gcgtcgaagt gaccggacca ccagcctggc
     5581 gtcaggagcg cggcaatcga tggcgcaccg ggcctgtatc ggggggtagc gccgcctcac
     5641 gctcgcgcgc tgcgacgacg gcggcctcga gcgagacctc ccccggaatt gccaagaaag
     5701 ccgcactcga caccgcgtca cgacaaggaa ctttcgggaa gccgtggtcg cctactgtgt
     5761 gcaagaagcg cagcgtgcga agcccatcga accaagtgtg cacgcgacga cgcaaatcac
     5821 cggcgcctac ctcggcgatc gcgccacgcg ctgcttcgag tacgccgagc tcgccagccg
     5881 ctgcgtcggc cgctgtcgct agatcgcgcg ggaggtccgt gaacgctcgc gcga
//
LOCUS       DASTKS010000853         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1028051, whole genome shotgun sequence.
ACCESSION   DASTKS010000853 DASTKS010000000
VERSION     DASTKS010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028051"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /gene="feoB"
                     /locus_tag="VFQ35_17700"
     CDS             complement(<1..1585)
                     /gene="feoB"
                     /locus_tag="VFQ35_17700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET9932544.1"
                     /translation="MTDEAVSEEQKTTGAAPSPRAAATEEPLVVLIGNPNVGKTALFN
                     RLTGQNARIGNYPGITVERRSGTVKHDLVASFEVIDVPGTYSFAARSAEEQIAIHAVL
                     GIGDHPKPALCVAVIDASQLARNLYLVLQLAEFGVPFVLALNMIDEVKDNPPDVAALE
                     RLLGAPCVATSARTGQGVPELKSAIARALLDPPRATMHVSYPPELIADVDRVAEALPS
                     WARDGSVERKRALALWALSSVEPDDELEGIPAELRERCLDVQRSAKHRDLDGEIVGTR
                     WEFIDRKLPELYARLDLHPPKRKLSARVDRVLLHPVLGFVAFLCVMLVVFQALFSWSD
                     PAVSLIEDAFRWLSLALQAHLPAGVLRDLLTVGVIGGVGNVLVFLPQILLLFFFIGIL
                     EDSGYMARVAYLMDRVMKALGLHGRAFVPMLSGFACAVPAIMATRTMERQRDRLLTML
                     VIPLMTCSARLPVYSLIAAALFPAGMKLGGILSVQSALMVGMYLFSLLTTLGAAAVLG
                     RTVVKARRVPLLLELPPYRI"
     gene            complement(1599..1925)
                     /locus_tag="VFQ35_17705"
     CDS             complement(1599..1925)
                     /locus_tag="VFQ35_17705"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET9932545.1"
                     /translation="MNLAKLPLGSVARVTRVGGTGSFRRRLMELGILPGTRVEVVSAA
                     PLGDPLELLVRGASLSIRKEQAESVEVNALPADLRVEDLATRSATVELDRGSWFSKLR
                     RWSPSK"
     gene            complement(2018..>2314)
                     /locus_tag="VFQ35_17710"
     CDS             complement(2018..>2314)
                     /locus_tag="VFQ35_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932546.1"
                     /translation="MWEALTGRRLFQADTDLDVVELVKQCRVPLLSRERPQLPLGLTS
                     AVHRALERDPLRRFASARAMLESLTEVLRVLPRSVDSRVIAASIRQLGLGARLG"
BASE COUNT          427 a          733 c          780 g          374 t
ORIGIN      
        1 ggatgcggta cggcggaagc tcgaggagga gcggcacacg tcgcgccttc accaccgttc
       61 gaccgagcac ggcggccgct ccgagcgtcg tgagcagcga gaacaagtac atccccacca
      121 tcagcgcgct ctgcacgctg aggatgccgc cgagcttcat cccggcgggg aacagcgcgg
      181 cggcgatgag gctatatacc ggcaagcgtg ccgagcacgt catcagtgga atcaccaaca
      241 tggtgagcag acgatcgcgc tgacgttcca tcgtccgcgt ggccatgatg gcgggcacgg
      301 cgcacgcgaa gccggagagc atcggcacga acgcgcgccc gtggaggccg agcgccttca
      361 tcacccggtc catcaggtac gcgacgcgcg ccatgtaacc gctgtcttcc agaatgccga
      421 tgaagaagaa cagcagcagg atttgaggca gaaagacgag gacgtttccg actccgccga
      481 tgacgccgac ggtcaagagg tcgcgcagga cgcccgcggg caggtgcgct tggagcgcga
      541 gtgacaacca gcgaaaggcg tcttcgatca gcgagacggc ggggtcggac caggagaaca
      601 acgcctggaa cacgacgagc atcacacaga gaaacgctac gaacccgagc acggggtgta
      661 aaaggacgcg atccacgcgc gcggagagct tgcgcttcgg gggatgcaga tcgagccgcg
      721 cgtagagctc tggcagtttg cgatcgatga actcccagcg cgtgcccact atctcgccgt
      781 cgaggtcgcg gtgcttcgcg gaacgttgaa cgtccaggca gcgctcgcga agttcggcag
      841 ggatcccttc gagctcgtcg tcgggctcga cactcgagag agcccacagc gcgagcgccc
      901 gcttgcgctc gacgctgccg tcgcgggccc agctcgggag agcctccgcc acgcggtcta
      961 cgtctgcgat gagctccggc gggtagctca cgtgcatggt ggcgcgtggc ggatcgagca
     1021 gcgcccgcgc aatcgcggat ttcagctccg ggacgccctg tccggtgcga gcgctcgtcg
     1081 caacgcaggg cgcaccgagc aggcgctcta gcgccgcgac gtctggtgga ttgtccttca
     1141 cctcgtcgat catgttgagc gccagcacga acggcactcc gaactccgcg agctgcagca
     1201 cgaggtacag atttcgcgcg agctggctcg cgtcgatgac ggcgacgcac aatgcgggct
     1261 tcgggtggtc accgatgccc agcaccgcgt gaatcgcgat ttgctcctcg gccgagcgcg
     1321 ccgcaaatga gtacgttccg ggaacgtcga tgacctcgaa gcttgcgacc aagtcgtgct
     1381 taaccgtccc cgagcgacgc tcgaccgtga ttcccgggta gttgccgatg cgcgcgttct
     1441 gccccgtgag tcgattgaag agggcggtct tgccgacgtt cgggttgccg atgaggacga
     1501 ccaagggctc ttcggtcgca gcggcgcgcg gcgaaggcgc ggcgccagtc gtcttctgtt
     1561 cctcggagac cgcttcgtca gtcatcgccg ttgccgcttc acttactcgg cgaccagcgg
     1621 cgcagcttgc tgaaccagct tccgcgatcg agctcgacgg ttgcgctcct cgtcgcgagg
     1681 tcttcgactc tcagatcggc aggcagcgcg ttcacctcga cgctttcggc ctgctccttg
     1741 cggatcgaca ggcttgcgcc tcgaaccaag agctcgagcg ggtcgccgag gggcgcggcg
     1801 ctgacgacct cgacgcgcgt gccgggcaag atgccaagtt ccatcaagcg ccgtcgaaag
     1861 gagccagtcc ctcccacccg tgtgactcgc gcgacggatc cgagcggcag ctttgctagg
     1921 ttcatgtagg tgttactcta tcctttttga aatcgacttt caaattcata cacggggcac
     1981 actggcggac acttgaattt acggccgttt ctatgggtta gccaaggcgg gcaccgaggc
     2041 cgagctgacg gatgctagcg gcgatgacgc gcgaatcgac cgaccgcggg aggacccgca
     2101 gcacttcggt gaggctttcc agcatggcgc gagccgacgc aaaacggcgg agcgggtcgc
     2161 gttcgagggc ccgatgcacg gcggaggtga gcccgagcgg aagctggggt cgctcgcggc
     2221 tgagcagcgg gacccgacat tgtttcacga gctcgacgac gtcgaggtcg gtgtcggctt
     2281 gaaacagacg gcggccggtg agcgcctccc acat
//
LOCUS       DASTKS010000854         6248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_804897, whole genome shotgun sequence.
ACCESSION   DASTKS010000854 DASTKS010000000
VERSION     DASTKS010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804897"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VFQ35_17715"
     CDS             <1..1845
                     /locus_tag="VFQ35_17715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932547.1"
                     /translation="GVLGMIELLHGTQLNEKQRRYAQTLQASANGLMTVLNDILDFSK
                     IEAGKLELHPGTSKVRDIMEEVGELFAARAHLKRIELVCHTDRNLPPFIKVDRDRLKQ
                     ILANLTGNAVKFTEKGQVALRALGDTRQGRATIRFEIADTGIGISEEAKSRLFGAFSQ
                     VDGSLTRKYGGTGLGLAICRQLVTLMGGEIGVDSTPGVGSTFWFWLPLQEAEVEDSDN
                     ARPTVKVRTLIVDDNATNRFVLEELLTSWNIEHASAADAASAMKLIEASSRENTPFGL
                     MITDMNMPEVDGLSLARQVLENGKGPKPAIVLLTSQSEDALPVGAELFVDAFMQKPVR
                     ADDLATCIGRILNSKPPQKSSKRKLNPKSTPAPKGERRLLVVEDNPINQEVMTEILRE
                     LGYSADVADNGQRALEMLEKRSYPLVLMDCQMPVLDGYQTTTQIRQNEDPGHRIPIIA
                     VTAHALAEERQKVLAIGMDDYISKPVSQQALSEVLERWWPDELDATHPPKQEEKAEEP
                     AGMRPPASALDPSVKRSDRVVRLFLETVPRDIQAIEAAILTGDPSLVKQAAHRLKGGC
                     LAIGVPRMASVCVQLEGNPPNRAELLTELCQEFDRARARLEGMLSRAN"
     gene            1993..2661
                     /locus_tag="VFQ35_17720"
     CDS             1993..2661
                     /locus_tag="VFQ35_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932548.1"
                     /translation="MNTAMYREKPRIRPRYVAALFALFGGAFASACTFDSSDRCGPNQ
                     VIFGDNLRCVCDTHSAYTATGCVRCGEHEVAGPSGCVCEPNFSKGTGGVCEPTPMGIG
                     VPCDGTCEDPVYKHCETTASGGYCTNTGCTGPADCTGGYACDTATGVCKRPPIGLAKS
                     CTSNADCAGTEATYCDTFVTHSCLVQGCTLAPDNCFQGWECCDLSSFGIPQPLCVLAG
                     ACTT"
     gene            2658..4133
                     /locus_tag="VFQ35_17725"
     CDS             2658..4133
                     /locus_tag="VFQ35_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932549.1"
                     /translation="MTARKLIRAGFAANIVFSIALSSVLARAQTAPPPEPSPYPPAAT
                     APSTEPAAAPSVAPTAPAAVAPATPAATPAPSDAADAAADAAAADAAAAAAAELASNP
                     GNTDTGEGFKLNIYGFADFTYSKAFKAPFTEGSQPSSFAVGKLNLYAGAELGDNWRSL
                     AEIRFMYLPNGTYETVPGATAAPPRTDTSAGDYTDLGRPVRWGGISIERAWLEHTFHP
                     AFTVRVGSWLTPYGIWNVDHGSPVIIGVRRPFIIGEALFPEHQTGVEAYGALNFGPSQ
                     FGYHLTLSNGRGPIDTYQDLDNNKGVGARLFFKHDSSFGTLTVGASGYRGKYTDSKTE
                     YAFNSKGDFVLAQPVTARYDELSLAMDVKYEYSGLLVQGEAILNDVAYVDKYRPAAFA
                     AAVPGPPGFNPDYRRLGVYGLAGYRTSFFGIMPFFGGEYYDMGLYSFQPPAAAVWGGI
                     NARPTARVVLKAQFTHSWYNEWAGQAPGNFNGLDLQAAWSF"
     gene            4133..4627
                     /locus_tag="VFQ35_17730"
     CDS             4133..4627
                     /locus_tag="VFQ35_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932550.1"
                     /translation="MQTRRSFLTAFIASLSLLATASLSAPVGAGEPRVKLAVVVAKDS
                     PVTDISIYELKRLYLGERINAAGKRLVPFNLAPLSRERSGFDKTVLGMSPDAIARYWV
                     DRKIRGESGPPKTVEPADVLQRVVVKLDGAVGYTPLNEMRPDVKAIRIDGKGPNDPGY
                     PLEF"
     gene            complement(4719..5792)
                     /locus_tag="VFQ35_17735"
     CDS             complement(4719..5792)
                     /locus_tag="VFQ35_17735"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9932551.1"
                     /translation="MKLRRSRWLLLGLICACNKAQTNEPSTTPTVAAVTSAPAPAVAS
                     SPSATPAPSAAPASSSATSANDAAAAKEAQGERDWAKIESERQAELARWTPEMHAAAK
                     KLAETSYASADAALKTLLASPHRHPGNAERDKYRHPRESLEFFGVKPTSTVLEYGPGA
                     GWYTELLAPLLAKHGKLYVTTEDPNGPHSDGATQGAKRLQNLFDTAPELFGKVDKIQI
                     DAKAPKLPLDSKLDFVLAIRVLHGMVNRNTLDAWLAEFKHALKPKGVLAVVEHRAPVG
                     ADPLASSKKGYVPEAYAIERIEAAGFKLAAKSEINANPKDTKDYPEGVWTLPPTYRLG
                     DKDREKYAAIGESDRFTLRFVKQ"
     gene            5867..>6248
                     /locus_tag="VFQ35_17740"
     CDS             5867..>6248
                     /locus_tag="VFQ35_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET9932552.1"
                     /translation="MARLYDPLRVAEVTLENRIAVSPMCQYSATDGFANDWHLVHLGS
                     RAVGGAGLVITEATAVEPRGRISPVDLGIWSDEHVPGLERIVRFIEAQGSVAGIQIAH
                     AGRKASTAAPWEGGHPLSLAAGGWE"
BASE COUNT         1151 a         2034 c         1944 g         1119 t
ORIGIN      
        1 ggcgtgctcg gaatgatcga gcttctgcac ggcactcagc tgaacgagaa gcagcgccga
       61 tacgcgcaga ccctgcaggc gtccgcgaac ggcttgatga cggtgttgaa cgatatcctc
      121 gacttttcca aaatcgaggc cggcaagctg gagctccatc cagggaccag caaggtgcgc
      181 gacatcatgg aagaagtcgg cgagctcttc gccgcgcgcg cgcacctcaa acgcatcgag
      241 ctcgtctgcc acacggatcg caacctcccg cccttcatca aggtcgatcg cgaccggctg
      301 aagcaaattc tcgcgaacct caccggtaac gcggtcaagt tcacggaaaa gggccaggtt
      361 gccctgcgcg ccctcggcga tacgaggcag ggccgcgcga ccatccggtt cgagattgcg
      421 gacaccggaa tcggcatctc cgaagaagcc aaatcgcgcc tgttcggtgc gttttcgcaa
      481 gtcgatgggt cgctcacccg caagtacggc ggcaccggcc tcgggctcgc gatttgccga
      541 cagctcgtca cgctgatggg cggcgaaatc ggggtcgata gcacccccgg tgtcggcagt
      601 accttttggt tttggctgcc gttacaggaa gccgaagtag aggactccga caacgctcgt
      661 ccgacggtga aggtgcgcac gctcatcgtc gatgacaacg cgaccaaccg gttcgtgctc
      721 gaagagctcc tcacgagctg gaacatcgag cacgcgagtg cggcggacgc cgcttcggcg
      781 atgaagctca tcgaggcgag cagccgagag aacacgccgt tcggcctgat gatcaccgac
      841 atgaacatgc cagaggtcga cggcctcagc ttggcgcggc aggtcctcga gaacggtaaa
      901 ggaccgaagc cagcgatcgt gctgctcacc tcgcagagcg aagacgcgct gccggtgggc
      961 gccgaactct tcgtcgacgc cttcatgcaa aagccggtac gcgccgacga cctcgcgacg
     1021 tgcatcggga gaatcctgaa cagtaagccg ccgcaaaaga gcagcaagcg caagctcaac
     1081 ccgaagagca ctcccgctcc caagggcgaa cggcggctcc tcgtcgtcga agacaacccc
     1141 atcaaccaag aagtgatgac cgaaatcttg cgcgagctcg gctacagcgc cgacgtcgcg
     1201 gacaacggac aacgcgcctt ggaaatgctc gaaaagcgct cgtatccgtt ggttttgatg
     1261 gattgtcaga tgccggtgct ggatggctat cagacgacga cgcagattcg tcagaacgaa
     1321 gaccctggtc atcgaatccc gatcatcgcc gtcacggcgc acgcgctggc cgaagagcga
     1381 cagaaggtgc tcgcgatcgg catggatgac tacatctcga agccagtcag ccagcaagcg
     1441 ctgtccgagg tgctcgagcg ctggtggccc gacgagctcg atgcgacaca cccgcccaag
     1501 caagaagaaa aggcggagga gcccgcgggg atgcgaccgc cggcctccgc tctcgacccc
     1561 agcgtaaagc ggagcgaccg cgtcgtgcgc ttgttcctgg agacggtgcc gcgcgacatt
     1621 caagcgattg aagcggcgat tctcacgggc gatccgtcgc tcgtgaagca agccgcccat
     1681 cgcttgaaag gcggctgcct cgcgatcggt gtccctcgca tggcgagcgt ttgcgtgcag
     1741 ctcgaaggta atccgccgaa ccgcgctgaa ctgctgaccg agctttgcca agagttcgac
     1801 cgagcccgcg cgcgactcga agggatgctg agccgcgcga actagttcgt ggctggggat
     1861 tgcgctgcac caattcgttc aagaatttcg acgtacggtc accactaaac tctgaaacac
     1921 aggcgccgtt gttcgctgcg gccctcgatt gagatcgttg acgttctggg acctcgctcg
     1981 tccggagatg gaatgaacac agctatgtat cgcgagaaac cgcggattcg accccgctat
     2041 gttgccgcgc tgttcgcgct tttcgggggt gctttcgcca gcgcttgcac cttcgattcg
     2101 tccgatcgtt gcggcccgaa ccaggtgatt ttcggcgaca acctacgctg cgtgtgcgac
     2161 acccacagcg cgtacaccgc gaccggctgc gttcggtgcg gcgagcacga agtggcgggc
     2221 ccctctggct gcgtctgcga gcccaacttc agcaaaggga ccggcggcgt ttgcgagccc
     2281 accccgatgg gaattggcgt gccgtgcgac ggaacgtgcg aagacccggt gtacaagcac
     2341 tgtgagacca ccgcgtccgg tggctactgc acgaacaccg gctgcaccgg ccccgccgat
     2401 tgcaccgggg gctacgcgtg cgacaccgcc accggcgtgt gcaagcggcc cccgattggt
     2461 ctcgccaaga gttgcacgtc gaacgcggac tgcgctggaa cggaagccac ttactgcgac
     2521 acgttcgtca ctcattcatg cctcgtgcaa ggctgcacgc tggctccgga caactgtttt
     2581 cagggttggg agtgctgtga cttgagctcc ttcggcatcc cccagccact ctgcgtcctc
     2641 gcgggagcct gcaccacatg accgctcgca aactaatccg cgctggcttc gcggcaaaca
     2701 ttgtgttttc catcgcgctc tcctccgtac tcgcacgagc gcaaacggcg ccgccgccgg
     2761 agccttctcc ctatcccccg gccgcaaccg cgccgagcac ggagccagca gcggctccca
     2821 gcgttgcgcc gactgcgccc gctgccgtcg cgcccgctac gcctgccgcg actccggccc
     2881 cgagtgacgc cgctgatgcc gccgcagacg ctgccgccgc agacgctgcc gctgccgctg
     2941 ccgccgagct cgcttcgaat ccggggaaca cggataccgg cgaaggcttc aagctgaaca
     3001 tctacggctt cgcggacttc acctactcga aggcgttcaa ggccccgttc accgaaggct
     3061 cgcagccgtc gtcgttcgcg gtcggcaagc tcaacctgta cgctggcgcc gaactcggcg
     3121 acaactggcg ctctcttgcc gaaattcgct tcatgtactt gccgaacgga acctacgaaa
     3181 cggttccggg cgcgaccgcg gctcctccgc gaacggacac cagcgctggg gactacaccg
     3241 acctcggccg cccggtgaga tggggcggga tctccatcga gcgcgcctgg ctcgagcaca
     3301 cctttcaccc cgctttcacg gtgcgcgtcg gctcttggtt gaccccgtac ggcatttgga
     3361 acgtcgacca cggctcaccg gtgatcatcg gtgtgcggcg cccgttcatc atcggtgagg
     3421 cgctcttccc ggagcaccaa acgggtgtcg aagcttacgg cgccttgaat ttcggaccgt
     3481 cgcagttcgg ctatcacctc acgctctcga acggacgcgg cccgatcgat acctaccaag
     3541 acctcgacaa caacaagggc gtcggcgccc gactcttctt caagcacgat tcgagcttcg
     3601 gcacgctcac ggtcggcgcc tcggggtatc gcgggaaata cacggatagc aagaccgagt
     3661 acgcgttcaa ttccaagggc gatttcgtct tggctcagcc ggtgacggca cgctacgacg
     3721 agctcagctt ggcgatggac gtgaagtacg agtactcggg gctgctcgtg cagggcgagg
     3781 ccatcctcaa tgacgtggcc tacgtggaca agtaccgccc cgcggccttt gccgctgccg
     3841 tcccgggccc gcccgggttc aacccagact atcgccgcct cggcgtttat ggtttggccg
     3901 gttaccgaac gtcgtttttc ggaatcatgc cgttcttcgg gggagagtac tacgatatgg
     3961 ggctctactc gtttcagccg ccagcagctg cggtttgggg cggcatcaac gcgcgaccca
     4021 cggcgcgcgt cgtcttgaag gcgcagttca cccactcctg gtataacgag tgggcaggac
     4081 aggcaccggg caatttcaac ggtctcgacc tccaagcggc atggagcttc tgatgcaaac
     4141 gagacgatct ttcctcactg cgttcatcgc ctcgctttca cttctcgcga ccgcatcgct
     4201 gagcgcgcca gttggcgccg gcgagcccag ggtgaagttg gccgtggtcg tcgcgaaaga
     4261 tagccccgtg accgacatct cgatctacga gttgaagcgc ctctatcttg gcgagcgcat
     4321 caacgccgcg ggtaagcgct tggtgccatt caacctcgcg ccactcagcc gcgagcgctc
     4381 gggcttcgac aaaaccgtgc tcggcatgtc acccgacgcg atcgcgcggt actgggtcga
     4441 ccgaaagatc cgaggtgaat cggggccacc caagaccgtc gaacccgctg acgtgctcca
     4501 gcgcgtcgtc gtgaagttgg acggcgccgt cggctacact ccgttgaacg aaatgcgacc
     4561 cgacgtgaag gcgattcgga tcgacggcaa agggccgaac gacccgggct acccactcga
     4621 gttctgagga tcgaagagcc agccgattcg cacagatcgg ccggctctgc cttcgctcgc
     4681 ctcggttcgc cgaagcgcaa acgtgaagcg atcgctcgtc actgcttgac gaagcgcagc
     4741 gtaaagcgat cgctttcacc aatcgccgcg tacttctctc gatctttgtc gccgaggcga
     4801 taggtcggag gcagcgtcca aacgccttcg gggtagtcct tggtgtcctt cgggtttgcg
     4861 ttgatctcgg actttgcggc tagcttgaag cccgccgctt cgattctctc gatcgcgtac
     4921 gcctcgggaa cgtagccctt cttgctgctg gcgagcggat cggctccgac gggcgcgcgg
     4981 tgctcgacga cggcgaggac gcccttgggc ttgagggcgt gcttgaactc ggcgagccaa
     5041 gcgtcgagcg tgttgcgatt caccatgcca tgcagcacgc gaatcgcgag aacgaagtcg
     5101 agcttggagt cgagcggaag cttcggcgcc ttcgcgtcga tttggatctt gtcgaccttg
     5161 ccgaagagtt cgggcgcagt atcgaacaag ttttgtaggc gcttcgcccc ctgcgtcgcg
     5221 ccgtcgctgt gcggaccatt cgggtcttcg gtcgtcacgt agagcttgcc gtgcttcgcc
     5281 aagagcggcg ccaagagctc ggtgtaccaa ccggcgccgg gcccgtactc gagcaccgtc
     5341 gacgtcggct tcacgccgaa gaactccagt gactcgcgcg ggtgacggta cttgtcacgc
     5401 tcggcgttac cgggatggcg atgcgggctg gcgagcagcg tcttcagcgc cgcgtcggcg
     5461 gacgcgtacg aggtctccgc gagcttcttc gccgccgcgt gcatctcggg cgtccagcga
     5521 gcgagttcgg cttgacgctc gctctcgatc ttcgcccagt cccgctcgcc ctgcgcctct
     5581 ttggcggccg cggcgtcgtt ggcggacgtg gcggacgagc tggcgggcgc ggccgacgga
     5641 gctggcgtcg cggatggtga tgacgcaacg gccggcgccg gcgctgacgt caccgccgca
     5701 accgttggcg tcgtcgacgg ctcgttcgtt tgcgccttgt tgcaggcgca aatcaatcca
     5761 agtagcaacc agcgcgatcg gcgaagcttc atgcgcgcgg ttctactacg cggtaatctg
     5821 cgccacaacc aatcgacatc catcgactcc gacttaggac atcagcatgg cgagactgta
     5881 cgacccgctc cgtgtggcag aagtgacact cgagaaccga attgcggtct cacccatgtg
     5941 ccagtacagc gcgaccgacg gcttcgctaa cgactggcac ctcgtgcatc tcggaagccg
     6001 cgcggtgggc ggcgctgggc tcgtgatcac cgaagcgacc gcggtcgagc cgcgcgggcg
     6061 catttctccg gtagatcttg ggatttggag cgacgagcac gtgcccggcc tcgagcggat
     6121 cgttcgcttc atcgaagcac aaggttcggt agccggaatt caaatcgcgc acgctggtcg
     6181 caaagcttcg accgcagcac cctgggaagg cgggcatcca ctttctctgg ccgcaggcgg
     6241 ttgggaaa
//
LOCUS       DASTKS010000855        17844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_804968, whole genome shotgun sequence.
ACCESSION   DASTKS010000855 DASTKS010000000
VERSION     DASTKS010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17844
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804968"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VFQ35_17745"
     CDS             complement(<1..1072)
                     /locus_tag="VFQ35_17745"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9932553.1"
                     /translation="MKTLERKYRGSLSGGRLGGGVLGVVSIACLALLAAGCGGDDDGG
                     GSAGLPENLHIVPGSPASGANGSVEHPFATLTAAVDAIKAAATWDGVIVAHKGRHELT
                     VELALPKNADFKVLPGATFAMGPDVSLHVQRHLEVRGTEAEPVLFTWLQSGSHWGSLT
                     NFVKTSLDNVVEYATFEHGGESQFEGIGVRGALSFADAGGRISHCTFRENEGDDGVNL
                     KRSPTVVEYSTFEGNFGDALDSDGPADAEIHDCLFRDNGNDAVDLGEGSTEHVFHNIM
                     INSGDKGVSIGDTSFPYVHNNLMIGCHIGVGVKDSSDPEVRNNTIYAADKGVSSYENT
                     SGKGGGLGQFVNNIIWASKDVDV"
     gene            complement(1120..2562)
                     /locus_tag="VFQ35_17750"
     CDS             complement(1120..2562)
                     /locus_tag="VFQ35_17750"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9932554.1"
                     /translation="MKRVLSLVPLLTVLAAGCGDDSNSKNGGAAGSSGNDPGTAVFAR
                     KAPAGGCLADCGPTTEAFFNQEQLATLRLDFDPADLKTLGYEPSEWLDLLWSKWKSCN
                     ENFTWVPATLKYESPDGQGDVTMERVGVRLRGSKSRGTNALQGMKVDFKKLIPTPGAG
                     EEERRFADLNQINLLSVEKDTSIMLQCMSYELMRGLGVKAPRCNHINVVINGQLYGVM
                     QSVEKTNDARFLKHQFGDNDGHLYGGSTSCELENPYGTSLEYEGDTFTGDYLKSYEIV
                     RGDVGNAETELLPMFKCGDDVATPDDEEFKACISEWIDVDEWLRVIAGESLIPQLESF
                     IGARRNAYFYFLPDATAPHGGRFVVWGWDYDTGLQRATCNPSKPGGVGCDPFKAVAKW
                     FDLPGTRPKLVTRLTRVFKAQYCEIMQSFLDTVYNPDLVDVRASAMDTAMQTNIAPDY
                     ATWKPEVAKMRDYMVQNLAAEREIVAAACQ"
     gene            complement(2981..4315)
                     /locus_tag="VFQ35_17755"
     CDS             complement(2981..4315)
                     /locus_tag="VFQ35_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932555.1"
                     /translation="MKHKFGTRSIAVALWASLLAVGVASPAHAKKKVAGGGNAHVGAA
                     APSTPSEEESPNGNGNGTLRPEDNEPTVAETGDAFSDTKGPVGVYQFRTLLQTRFTHT
                     AVDLAPIQDRLTAEDPLNGNYFRDIVNERAHSDDGFAIQRAFLRATVQPTPIFSAKLL
                     VDFAEFVHKNQKAALKLAYGELRPTHSVSITAGLFKIPFSLLELLPIADFEFADVGPT
                     DALIKDMGVGGRDIGIKIQITPLAKRKSLSLEAGMYDGDNHNLQKNPGPGIMAARLKM
                     RPFSHLEVAGDCVYRPTNVRDLDDGGNPYQKHAKGGACSADASFSYHRFAFRGEYMTG
                     KRTDTPLFHDNKDHRFSAVWGLATYRVRLKKNMSLRPAVRVEWLDEDQPSNIGQTLAI
                     SAAMTLDLSNSTRILFDLTRARVQGGTRDRSNDGPLYRPSTTVGVAQLQFKL"
     gene            complement(4444..6522)
                     /locus_tag="VFQ35_17760"
     CDS             complement(4444..6522)
                     /locus_tag="VFQ35_17760"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932556.1"
                     /translation="MRTEGSRSRGSFRLWLGWLLTALFIGVYFALPSGDEAIPFALTP
                     ADTAEPSSGARQIEIVEVSPPRPSPGRAVTISYIGADEPAVHAFAGKIDMQILARSRG
                     VLVARVPETLPPGRIKLRLVAGAERSKAYALSLRAPNWQKPFRHLLGGFALLIFGIGV
                     LARGTREAVGFASAHTFARLAGRGPSVLGLGGVLGAIAQSTTAAAGILSGLVSSNLLA
                     VGPAAIAFLGAQFGAASAPLATGLIDTREGLIAIAVGVLWLNLGEDRRSRALARIVLG
                     AGLMAFGLQTFRPGFEALLQDATLLEFLSKLRADGMLHSVLCALLGAFLVAVLQGPAP
                     VIVLVILIAQTTAQWDLNTALSLLAGTGLGASLGALVTTPVSRRGRQLAVMNLVLGLA
                     STVIAVSTIHLWSHVADWIVPGVPHEVDWSRRGVQPNLAPHLGVAFVLSQVGAAVALL
                     PAAFPLSRWLDKRFAPAVSRSIAQVGDPVGVARAALIDVLHAQLGALAPVRELVSEGS
                     RSRGRLAEHRLAEGHAALDELLSAAVPALPDTADGRAIARAAFTSMQLQRGLEGVHRQ
                     AERLVDARIASSTTGSNVLALSEGDDVVLTQMHALLTEGLEALLTLLRERGGLDLDSV
                     RAREIQMNALEARARNQLLEGGREPSGVGSHLRVLELVDAYESSGNQLYRLAEALEES
                     VEASVARRVM"
     gene            complement(6589..8280)
                     /gene="tmk"
                     /locus_tag="VFQ35_17765"
     CDS             complement(6589..8280)
                     /gene="tmk"
                     /locus_tag="VFQ35_17765"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9932557.1"
                     /translation="MFVVFEGIDGSGKTTVSNQVVERLQAAGLKVRHLRAEGKFASRV
                     SESIRDLGRDARNLEIAPQTEFLLYVARDVQLIEEFLRPALAEYDVVIADRFLYTAEV
                     LGREGRRLPREFTEPVLRAAAGGIEPDLVVLVDVDPVLARARRKAHKIAVLDKRPPSR
                     KGLSGVGLQHRVRNGYRELARKDPSRWALVQNEEVLEDTVARVTELVARAVRSGVPSA
                     LAHFRDGAVPSARQHESVASPRAALDKFMQWIDLRLRREPRVAAYLLGGLYGPGIDER
                     RLALSEAVPEAVLAALNGLDDDVSWSLRERLAEKFPGAVARTLSSLAGLSPRAVEMRE
                     ALVSVAPAEVGSSLWRLEDERSWALRDRLFDEHPHVVAGSVAWLGSERAWALRDRWLS
                     AYGSRIGSDYDTARIAARSVTSLDDERAWKIRKQTMQAAPVAALWSVGGCTDEGSWRL
                     RAEYVTRAPKVVMGTLKGSRDERAWEMRRLVAEDCKEAIDSIQHLDDEPAWQLREQNQ
                     DRWPSTVVKSLGLLSDGERAQKLVLRQLDRHGNNISLLKHVSSIALGLRQQELDD"
     gene            complement(8345..9415)
                     /locus_tag="VFQ35_17770"
     CDS             complement(8345..9415)
                     /locus_tag="VFQ35_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932558.1"
                     /translation="MNNSSSLFGSSSRSTFGSLAPVLVVVGLGVAGITASGCSSDGDG
                     NGASGGAGSGGSSSGGAGGGASKAMYAPCSDATRIGTMTVSVVPKTSTGGGYSEFNGG
                     IYDSVDPRNVWTEKTKEGDCRLITGPKSMCAPACTSGQVCSLTTKQCVAEPEPQDAGT
                     VSVAGLSAAFSADYRAGVYYKPITGAFPPAAAGADVTMSAAGAKVPAFSITASGIDPL
                     QVPEGDITLDTSKPLTITWTPPTKAGTSHVVVSLDLAHHGNVAAKLVCDVADTGSTTI
                     PVSLLTALAAEGVAGFPSVAIVRRALNSATVGSGCVEFGVSSSLERPLVLPGVMSCMC
                     PNDTCEPCAAAGLTCKANYTCG"
     gene            9955..10284
                     /locus_tag="VFQ35_17775"
     CDS             9955..10284
                     /locus_tag="VFQ35_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932559.1"
                     /translation="MKNKLGWALAILVTGALTLGATGCGSDDGGGGGGDTAKSSCETF
                     CNKEKDCNTNTTVADCIMYSCSDIEKASAGCQSALKAFYDCMNKATNVCDSASCAAQE
                     TAYHDAC"
     gene            10310..11572
                     /locus_tag="VFQ35_17780"
     CDS             10310..11572
                     /locus_tag="VFQ35_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932560.1"
                     /translation="MTPHRPLLRSHAHGLPKGLLCAGIFVHLAALVFAGCGGSGGSSG
                     AGGTGVGSGGAPALGGANSSGGVAPTSGGTASGGTTTASGGASTASGGKTTGGGASSG
                     GASSSSGGKTGSGGANNAGTSSAQGGATSTGGVTSNGGTSAGCTDFQTPTKVGDIAET
                     ALDGPSGIAASRAHAGVLYAHLDAGGGAVVYAMTYAGKALGAYTLMGITATDWEDIAV
                     GPCANGDCIYIGDIGDNAARMGGTTRTQIQVLRVAEPNVSATQAATQQMLGDVTIMRF
                     TYPDKAHDAETLMVDPVTKDILIITKETDGNSSIFRAAANTPADMPTVLEKVGNVQIG
                     ASGQAAQASAGDISPTGDRFIVRSYTAILLWPRAATFAATFAATPKMVPSPTETQGEG
                     LTFTSDGKAWLSAGERSRAIYEGVASCP"
     gene            11689..12786
                     /gene="phoU"
                     /locus_tag="VFQ35_17785"
     CDS             11689..12786
                     /gene="phoU"
                     /locus_tag="VFQ35_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9932561.1"
                     /translation="MTHFEETLQRDVERIRSKVREMAALAEGSLNSCIAALVQRNRQI
                     AYSVILRDQRIDELDKEIDRLCLEFILRQQPVAGTLRFVYAAIKINSDLERVGDYAES
                     MARQGLSLFALEVDVPVDRVQEIANKAIPMLHGAVEAFVSQDADLAVKTMETEEVVDR
                     LRHQLSQDLVGLIQRQKMPLEALAPLMNIVNRLERVADQARNICQETLYMCTGEYMKH
                     AGSETFRLLFVDESNSCVSQMAEALGSIQQVPNFVFSSAGLSRKPVDPATVLFLRDKG
                     IDISRSVSKGIDQIPHLDHYQIIVALTKEAQRVFPPPPTKAVCLDWSLADPSQSTGTP
                     EQVRAAYDETYKFLKTHIRDLVQAILGDQHN"
     gene            12801..13748
                     /locus_tag="VFQ35_17790"
     CDS             12801..13748
                     /locus_tag="VFQ35_17790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9932562.1"
                     /translation="MKTYVGTLRFGCIALFALGVLSASCRKKTEPSEAQPAGSAESAV
                     AADNKKESIQNIGSDTMVNLAQAWAEEYAKVAPNVSIEVSGGGSGVGIAALINGTASV
                     ANASRKLEPEEAEKAKKASGKEPKEFMVGYDGLAIYVSKQNPLEEISLEDLSEIYKEG
                     GKINKWSELGVKSIPGAKSDDIVRVSRQNNSGTYHYFREVVIGKKADYKPGSLDMNGS
                     KDVVELVAKTPGAIGYSGLGYATPDVKIVKVSKKKGEPAITPSIATVLDKTYPISRPL
                     FMYTPGEPSPAVHKYLDWIMSDAGQKIVEHTGYVPLAKK"
     gene            13776..14741
                     /gene="pstC"
                     /locus_tag="VFQ35_17795"
     CDS             13776..14741
                     /gene="pstC"
                     /locus_tag="VFQ35_17795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9932563.1"
                     /translation="MKHEAALLGPAGRARTSLAPAQLAQKRRKVEAVTERILEALIRL
                     CGVSAILFVFGIFFFVLREGIGLLFDPAFSLRKFLFSTEWYPTSLSNVRYGVLSLIVG
                     TASVTVTAMLIAVPFGIGAAVFVSEFCSRRVKETLKVVIELLAAIPSVVWGFIGISVM
                     NPLIQDVFHVPIGLNVLNGALLLALMSVPIMVSIGEDALKAVPDSFREAAIALGATRW
                     QVVYKVLLPAAKNGLLAAALLGVGRAVGETMAVLMATGHAINIPHSVFDPVRTLTATI
                     AAELGEASVGSAHYQVLFLVGILLFSITFVVNLTADLIVKGVKKG"
     gene            14746..15630
                     /gene="pstA"
                     /locus_tag="VFQ35_17800"
     CDS             14746..15630
                     /gene="pstA"
                     /locus_tag="VFQ35_17800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9932564.1"
                     /translation="MFTATPLVHKKQRAELAAKVLFGAMALAMVIPLVFIVGYLVIKA
                     APLLSWEFLTTNPTGGMRHGGIWSALLGTIYLVTVSLLIAAPVGVLAAVYLNEYARES
                     VFTRIINLAVVNLAGVPSIVHALFGLGAFVLFAGIGRSILAASLTLAIMTLPVIIAST
                     KEALSAVPTSFREACWNVGATRWQTIRGIVLPNSVSGILTGVILQVSRTAGETAPIMF
                     TGAVFFKKIQEGDPFAYSIWDQCMALSMHLFTITTQVTDAPKALPYATAVVLLGCVLS
                     VNATAIGVRSYLRSRKKW"
     gene            15624..16400
                     /locus_tag="VFQ35_17805"
     CDS             15624..16400
                     /locus_tag="VFQ35_17805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9932565.1"
                     /translation="MVAPQAPLPAKIRVNDLRLAYGDKQVLNGISFDVQENEIFAIIG
                     PAQSGKSSLLRCLNRTIEFVPGAKAQGSIQIDGAEVSSIHNVYELRRKVGMVAPLPVG
                     LPLSIYDNVAFAPRAAGFRNKADLDELVQRCLTQAALWDEVKDRLNSLGTKLSGGQQQ
                     RLTIARALSHQPEILCLDEFSIAIDPVTTMRIEAVLKELKREMTIVLVTNLVQQARRL
                     ASRTMFLWNGDIVEIDKTDVIFSSKPKSQKTYDYVNGIFG"
     gene            16397..17188
                     /locus_tag="VFQ35_17810"
     CDS             16397..17188
                     /locus_tag="VFQ35_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9932566.1"
                     /translation="MKRAASIAEESAQEGIVTRALNLWYGDFQALKNIDLNIKYGQIT
                     SLIGPSGCGKTTLLRCFNRVNERYGYVRTTGEIRILGKNIYDSDVSLIELRKSVGMVF
                     QRPNPLPISIYDNVAFGMRIHNEKPARAVLDEAVERALVEVGLWDDLKDKLDERATSL
                     QLEQQQKLCIARLLPVKPEVILMDEPCSALDVEGTRAIEELMWALRGRYTIVIVTHNM
                     AQARRASDECIFMLLGELIEHRRTEELFLNPQNPKTAEYIEGRYG"
     gene            complement(17192..17458)
                     /locus_tag="VFQ35_17815"
     CDS             complement(17192..17458)
                     /locus_tag="VFQ35_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET9932567.1"
                     /translation="MCRNIRPLFNFEPPATDEEVHAAALQYVRKVSGTRTPTGHNLAL
                     FEQAVAQIAEITRNLVDSLETKAQPKNREEEAEKARARNLKRFG"
BASE COUNT         3358 a         5786 c         5639 g         3061 t
ORIGIN      
        1 cgacgtccac gtccttcgac gcccagatga tgttgttcac gaactggccg agaccaccgc
       61 cctttccaga cgtgttctcg tacgacgaga cgcccttgtc ggcggcgtag atggtgttgt
      121 ttcgcacctc gggatccgag ctgtctttga cgccaacgcc gatgtggcaa ccgatcatga
      181 gattgttgtg cacgtacggg aaggaggtgt cgccgatgga gacgcccttg tcgccgctgt
      241 tgatcatgat gttgtggaac acgtgctccg tcgagccctc gcccaaatcc accgcgtcgt
      301 tgccgttgtc gcgaaacaag cagtcgtgaa tttctgcgtc ggcggggccg tcggagtcga
      361 gcgcgtcgcc gaagttcccc tcgaaggtgg agtactccac gaccgtgggg ctgcgcttca
      421 agttgacgcc gtcgtcaccc tcgttctcac ggaaggtgca gtgcgaaatg cgaccgccgg
      481 cgtccgcgaa ggagagcgcg ccgcgcaccc cgatgccttc gaactgagac tcgccgccat
      541 gctcgaaggt cgcgtactcg accacgttgt cgagcgacgt cttgacgaag ttcgtgagcg
      601 agccccagtg tgaacccgat tgcagccaag tgaagagtac cggctcggcc tcggtgccgc
      661 gaacctcgag gtggcgctgg acgtgaaggc tcacgtccgg acccatcgcg aaagtagcgc
      721 cgggaagcac cttgaaatcc gcatttttcg gtaacgcgag ctcgaccgtg agctcgtgac
      781 gacccttgtg cgcaacgatc acgccgtccc acgtcgccgc cgctttgata gcgtcgacgg
      841 ctgcggtcag ggtcgcgaag gggtgctcga cgctgccgtt cgctccgctc gccgggcttc
      901 ccggtacgat gtgcaaattc tcgggcaaac ctgcgctgcc gccgccgtca tcgtcgccgc
      961 cacaaccggc cgccagaagc gcgagacaag cgatgctgac gaccccgaga actccgccgc
     1021 ctaaacggcc gccagaaaga cttccacggt acttacgttc gagcgtcttc acacacatcc
     1081 tccatcggcg ggctgggaca cctcgcgccg gtttcccagt cattggcaag cagcggccac
     1141 gatctcgcgt tcggccgcta gattttgaac catgtagtcg cgcatcttcg cgacctcggg
     1201 cttccaagtc gcgtagtcgg gggcgatgtt cgtttgcatc gcggtgtcca tcgcgctggc
     1261 acgaacatcg accaagtcgg ggttataaac ggtgtccaag aacgactgca tgatctcgca
     1321 gtactgggcc ttgaatacgc gtgtcaggcg cgtcacgagc ttggggcgag tccccggcaa
     1381 gtcgaaccac ttcgcgacgg ctttgaaggg atcgcagccg acgccccctg gcttgctggg
     1441 gttgcacgta gcgcgttgaa gaccggtatc gtagtcccag ccccacacga cgaagcgacc
     1501 gccgtgcggc gccgtcgcat cgggaaggaa gtagaaatac gcgttgcgcc gcgcgccgat
     1561 gaaggactcg agctgcggga tcagcgactc accggcgatc acacgtagcc actcgtcgac
     1621 gtcgatccac tcgctgatgc aggccttgaa ttcttcgtcg tcgggtgtag cgacgtcgtc
     1681 gccgcacttg aacatcggca agagctcggt ctcggcgttg ccgacgtcgc cgcgcacgat
     1741 ttcataggac ttgaggtagt cccccgtgaa cgtatcgccc tcgtattcga ggctcgttcc
     1801 gtagggattt tcgagctcgc aggaggtcga gccgccgtag aggtgaccgt cgttgtcacc
     1861 gaattgatgc ttgagaaagc gcgcgtcgtt cgtcttttcg acgctctgca tgacgccgta
     1921 gagctggccg ttgatgacga cgttgatgtg gttgcagcgt ggtgccttca cgccgagccc
     1981 acgcatcagc tcgtagctca tgcactgaag catgatgctc gtgtcttttt cgaccgaaag
     2041 caggttgatc tggttgaggt cggcgaaacg acgttcttcc tcccccgcgc ccggagtcgg
     2101 aatgagcttt ttgaaatcga ccttcatgcc ttggagcgcg ttcgtcccgc ggctcttgct
     2161 gccgcgcaag cgaacaccga cgcgctccat ggtgacgtca ccctgaccgt cggggctctc
     2221 gtacttgagc gtggccggca cccacgtgaa gttctcgttg caggacttcc acttgctcca
     2281 caaaaggtcg agccactcgc tgggttcgta gccgagcgtc ttcaggtccg cagggtcgaa
     2341 gtcgagccgc aaggtcgcga gctgctcttg gttgaagaac gcttccgtcg tcgggccgca
     2401 gtccgccaaa catccgcccg ctggggcctt gcgcgcgaag acggccgtgc ccggatcgtt
     2461 tccggaactc ccagcggcgc cgccattttt cgagttggaa tcatccccgc aaccggctgc
     2521 aagcacggtc agcagaggca cgagtgacaa gacacgtttc atggtgacgc ttctagcccg
     2581 cagatttggg gtcggtatgc gggtgcgctc ccgcagtggg gcatgcacaa gttagtaccc
     2641 gcagagaact gaacacaaca taaaatctag atggctaatg taaggcatct tcgtgtcgca
     2701 cggcacacag atagcggctt tcagcgaaag ttcgcagtgt gcgacggcac tccgcgggct
     2761 cgttgccgta cgcagcgagc aagttccact gcggcgtcac acgaaatctt cgaccaactc
     2821 gaagattgac ccgttttcgt cgattcatga atagtcgagt cgtcccgcga gcacccgcac
     2881 ggctcgtgag ttcgacgaat caacgacact ccgatgacga cgctgattca ggccgacagc
     2941 gcttcgcagc cgcgcccgaa ctgcgaaaaa tcagtgctac tcagagcttg aactggagct
     3001 gcgccacccc gaccgtggtg ctcggtcggt acaaaggccc atcgttcgag cgatcgcgag
     3061 tcccgccttg aacacgcgcg cgtgtgaggt cgaacaagat gcgcgtcgag ttcgagagat
     3121 cgagcgtcat cgccgcactg atggcgaggg tctgcccgat gttgctcggt tgatcttcgt
     3181 cgagccactc gacgcgcacg gccggcctga gtgacatgtt ctttttcagc cgtactcggt
     3241 acgtggcgag gccccacacc gcggaaaagc gatggtcctt gttgtcgtgg aagaggggcg
     3301 tgtcggtgcg cttgcccgtc atgtactcgc cgcgaaacgc gaatcggtga tacgaaaagc
     3361 tcgcgtcggc gctgcaggcg ccgcctttcg cgtgcttctg gtaggggtta ccgccgtcgt
     3421 ccaagtcgcg cacgttcgtg ggacggtaga cgcaatcacc cgccacctcg agatgcgaaa
     3481 acggccgcat cttgaggcga gccgccatga tgcctggccc gggattcttc tgcaggttgt
     3541 gattgtcacc gtcgtacatg cccgcctcca ggctcaagct cttacgtttg gcgaggggcg
     3601 tgatctgaat tttgatgccg atgtcgcggc cgcccacgcc catgtccttg atgagcgcgt
     3661 cggtgggacc cacgtcggcg aactcgaagt cggcgatggg caagagctcg agtagagaga
     3721 acgggatctt gaagagcccg gccgtaatgc tgaccgagtg cgtggggcgc agctcgccat
     3781 aagcgagctt aagcgccgcc ttctggttct tgtggacgaa ctcggcgaag tcgacgagca
     3841 gcttggcgct gaagatgggc gttggctgaa cggtggcgcg caagaacgca cgctggatcg
     3901 cgaagccgtc gtccgagtgc gcgcgttcat tgactatgtc gcggaagtag ttcccgttca
     3961 aaggatcttc cgcggtcaag cggtcttgaa tcggcgcgag gtccactgcg gtgtgcgtga
     4021 accgggtctg gagcagcgtg cgaaattggt agacgccgac cggacccttg gtgtcggaga
     4081 aggcgtcacc ggtttcggcc accgtcggct cgttgtcttc ggggcgaagc gtgccgttgc
     4141 cgttgccatt cggcgactcc tcctcggagg gcgtcgatgg agcagcggcc ccgacgtgcg
     4201 cgtttccccc gcccgcgacc ttcttctttg cgtgggcggg agaggccacg ccgacggcga
     4261 gcaagctggc ccataaggcg acggcgatcg atcgagttcc gaacttgtgc ttcatgatct
     4321 tctctcaaac tctgcccacg actcgtccgc tcaccgtcag agcgacgcgc cgagcgttcc
     4381 tcgcgcgcga ggagactcgt cgctcagcgc gaggggacga ccgatagcca cgcgccacgc
     4441 agctcacatc actcgccgcg caacgctggc ttcgacgctc tcttcgagcg cctcggccaa
     4501 cctgtacaac tgattgccgc tgctctcgta ggcatcgacc agctcgagca cgcgcagatg
     4561 gctccccacc ccggacggct cacgaccgcc ctcgaggagc tggtttctgg cgcgcgcttc
     4621 gagcgcgttc atctgaatct cgcgggctct caccgaatcc aaatcgaggc caccacgctc
     4681 gcggagcagg gtcaatagcg cctccaaacc ctcggtgagc aaggcgtgca tctgcgtgag
     4741 aacgacgtcg tcaccttccg agagggcgag cacgttgctg ccggtcgtgg agctcgcgat
     4801 gcgggcgtcg accaggcgct ccgcttgccg atgaactcct tcgagcccgc gttggagctg
     4861 catgctggtg aaggctgcgc gcgcgatcgc gcgcccatcg gcggtgtcgg gcagcgcagg
     4921 cacggctgca gacaagagct cgtcgagggc ggcgtgcccc tcggcgaggc ggtgctcggc
     4981 gaggcggccc cgcgagcgac ttccttcact gacgagctcg cgcacggggg cgagcgcacc
     5041 gagctgggcg tggagcacgt cgatgagcgc cgcgcgcgcg acccccaccg gatctcccac
     5101 ttgcgcgatc gatctcgata ccgccggcgc gaagcgcttg tcgagccagc gcgagagcgg
     5161 gaaggccgcc ggcaggagcg ccaccgccgc gcctacctgc gagagaacga acgctactcc
     5221 gaggtgcggc gcgaggttcg gttgaacacc acgccgcgac cagtcgactt catgtggcac
     5281 gcccggcacg atccagtcgg ctacgtggct ccacaggtgg atcgtcgaca ccgcgatcac
     5341 ggtgctggcg aggccgagca ccaagttcat caccgcgagc tgtcgaccgc ggcggctgac
     5401 cggcgtcgtc acgagggccc cgagcgaagc gccgagcccg gtccccgcga gcaatgacag
     5461 cgccgtgttg agatcccact gcgccgtagt ttgcgcgatc aagatcacga ggacgatgac
     5521 cggcgcgggg ccctgcagca ctgccaccaa gaaagcgccg agcaacgcgc agagcacgga
     5581 gtgcagcatt ccgtccgcac gcagcttgga cagaaactcg agcagcgtcg cgtcttgcag
     5641 caaagcctcg aaccccggcc gaaacgtctg gaggccgaat gccatcaggc cggcgccgag
     5701 cacgatgcgc gcgagcgccc gggagcgcct atcctctccg aggttgagcc agagcacacc
     5761 aaccgcgatg gcaatcagcc cttcgcgcgt gtcgatgagg ccagtcgcga gcggcgcgct
     5821 ggcggcgccg aactgagcgc ccaagaacgc gatcgcggcg gggcccaccg cgaggaggtt
     5881 cgacgagacg agaccagaaa ggatacctgc ggctgcggtc gtcgactgcg cgatcgcgcc
     5941 gagcactcca ccgagtccga gcaccgaagg ccctctgccc gcgagcctcg cgaaggtgtg
     6001 agcgctcgcg aatcccaccg cttcgcgcgt tccgcgcgcg aggactccga tgccgaaaat
     6061 gaggagcgca aagccgccga gcagatgccg gaacggcttc tgccagttcg gcgctcgcaa
     6121 ggacaacgca taggctttgc tgcgctcggc gcccgcaacg agcctcagct ttatcctccc
     6181 gggcggcaga gtctccggca cacgcgccac gagcacaccg cggctgcgcg ccaagatctg
     6241 catgtcgatc ttgccggcaa aggcgtgcac cgccggctcg tcggcgccga tgtacgaaat
     6301 cgtgaccgcg cgaccagggc tcggccgcgg cggcgacact tcgacgatct cgatttggcg
     6361 cgcgccgctg ctcggttcgg ccgtgtcggc cggtgtgagc gcaaacggga ttgcttcgtc
     6421 accgctcggg agcgcgaaat agactccgat gaacagcgcg gtcagcagcc aaccgagcca
     6481 gagccgaaag ctacctcgcg aacgcgagcc ttcagtgcgc accgaatgca ccgccaatcg
     6541 ccgagcaaac ggtggtctcg tcgtcgacgc gggagccgcg acttctactc aatcgtcgag
     6601 ctcctgctga cgcaagccga gcgcgatcga agagacgtgc tttaggagcg atatgttgtt
     6661 gccgtgacgg tcgagctggc ggagcaccag cttctgggcg cgctcgccgt cgctcaggag
     6721 ccccaggctc ttcacgacgg tcgagggcca gcggtcttga ttctgctcgc gcagctgcca
     6781 tgcgggctcg tcgtcgaggt gctgaatgct gtcgatggcc tctttgcagt cctcggctac
     6841 gaggcggcgc atttcccagg cgcgctcgtc gcgcgagcct ttgagcgtcc ccatgacgac
     6901 tttgggcgcg cgcgtgacgt actcggcacg gagccgccaa ctaccttcgt cggtgcagcc
     6961 gcccaccgac cacaaggccg cgaccggcgc cgcctgcatc gtctgcttgc ggatcttcca
     7021 ggcgcgctcg tcgtcgagcg aagtcaccga gcgcgccgcg atgcgcgcgg tgtcgtaatc
     7081 ggagccgatg cgcgagccat aggcggagag ccagcggtcg cgaagtgccc aggcgcgctc
     7141 ggagccgagc cacgcaacgc tacccgcgac gacatgagga tgctcgtcga acaagcgatc
     7201 gcggagcgcc cagctgcgct cgtcttcgag gcgccagagc gacgaaccaa cctccgccgg
     7261 agcaaccgaa accaaggctt cgcgcatctc gacggcgcgg ggagagagcc ccgccagcga
     7321 cgagagcgtc cgcgcgacgg cacccgggaa cttctcggcg agtcgttcac gcaaagacca
     7381 gctgacgtcg tcgtcgaggc cattcagcgc ggccaacacg gcctcgggca ccgcctcgga
     7441 gagcgcgagc cgccgctcgt cgataccagg accgtatagt ccaccgagaa ggtacgccgc
     7501 gacgcgcggc tcgcgccgca gccgcagatc gatccactgc atgaacttgt cgagtgcggc
     7561 gcgtggagaa gccacgctct cgtgctgacg ggccgaagga acggcgccgt cacggaagtg
     7621 cgcgagagcg ctcggtacac ccgaacgcac ggcgcgcgcg acgagctcgg taacgcgagc
     7681 gacagtgtcc tcgagcacct cctcgttttg aaccaatgcc cagcgcgacg gatccttccg
     7741 agcgagttcg cggtagccat tgcgaacgcg gtgctggagc ccgaccccgg agagcccctt
     7801 gcgcgacggg ggccgcttgt cgagcaccgc gatcttgtgc gccttgcggc gcgcgcgggc
     7861 gagcacgggg tcgacatcta ccagcacgac cagatccggt tcgatgccgc ccgcggccgc
     7921 gcgcagcacc ggctcggtga actcgcgcgg caaccgacgc ccctcgcggc ccagaacttc
     7981 ggccgtgtac aagaagcgat ccgcgatgac gacgtcgtat tcggcgagcg ccggtcgcag
     8041 aaactcctcg atgagctgca cgtcccgcgc gacgtagagc agaaactccg tctgcggagc
     8101 gatttccagg tttcgtgcgt cccgccccaa gtctcgaatc gactccgaga ccctcgacgc
     8161 gaactttccc tcggcgcgca ggtgccgcac cttgagccct gcggcttgaa ggcgctcgac
     8221 cacctgattg gagacggtgg tcttaccgct gccgtcgata ccttcgaaga ctacgaacat
     8281 ggaagtttgg gctcctgatg cggctacgct cggcgggtcg gttgacccga atgaattgat
     8341 tcgatcaccc gcacgtgtag ttggccttgc aggtcaagcc tgccgcggca cacggctcac
     8401 acgtatcgtt cggacacatg cacgacatga cgcccggtag gacgagcggg cgctccagcg
     8461 aagacgacac cccgaactcc acacagccac tgccaaccgt cgccgagttg agagcccggc
     8521 gtacgatcgc gaccgatggg aagccggcga cgccttcggc cgcgagcgcg gtcaagaggc
     8581 tcaccggaat cgtcgtgctg cccgtgtccg ccacatcgca gacgagcttg gccgcgacgt
     8641 tgccgtgatg cgcgagatcg agcgaaacga cgacatgcga cgttcctgct ttcgtgggcg
     8701 gagtccacgt gatggtgagc ggcttggagg tgtcgagcgt gatgtcacct tccggtacct
     8761 ggagcggatc aatgccgctg gcggtgatcg aaaacgccgg aactttcgcg cccgcggcgc
     8821 tcatcgtgac gtcagcgccc gcggcggctg gggggaaggc gccggtaatt ggcttgtaat
     8881 acacgcccgc cctgtagtcg gcgctgaagg ctgcgctcaa gcccgcgacg ctcacggtcc
     8941 cagcgtcttg cggctcgggc tccgcgacgc actgtttggt ggtgagcgaa catacctgac
     9001 ccgaggtgca agctggtgcg cacatcgact tgggcccggt gatcaaccgg caatcgcctt
     9061 ctttggtctt ctccgtccag acgttgcgag gatcgacgga atcgtagatc ccgccattga
     9121 actcgctgta gccgccgccg gtggatgtct tcggtacgac ggagacggtc atcgtgccga
     9181 tgcgcgtcgc gtccgagcac ggagcgtaca tggctttgct cgcgccacct ccagctcctc
     9241 cacttgagct gcctccgctt cccgcaccac cggaggctcc gttgccatcg ccgtccgaac
     9301 tgcaaccaga cgccgtgatt ccggcgacgc cgaggcccac cacaacgagc acgggagcga
     9361 gcgaaccgaa agtcgaacga gaagaagaac cgaagaggga cgaggagtta ttcatttttg
     9421 actctgaggt caccttagcg tgaagaaaac tgtcaacgaa cttgaccgcc gcctctgcaa
     9481 ccacgcaaaa ttattgcact tacggaattg gtagggcgtg gcgaactgga gtcgtcggac
     9541 ggcccggaag cagctccggg cgcagcgctt cgtaagatga ccacgaggtt cctaccgcga
     9601 atagtagctg aaaagacacg aatacgtcac atgccgtttc cggcggcccg ccgggcgccg
     9661 aaaaacgtca gcattgctgg ttgtcggtgt tcaacttttg agggtcgcgc gaccgaagca
     9721 acttgggctt ggagcagccc gcgactctac aaataatgag gacgagcgtc gctgtctggt
     9781 cgacgacttt cagcacaaat cgcgccagac tcgataattc cgccgaggcg ccggtatgtg
     9841 acgtcctcaa tgccaattct tgcaagttca gactggcacg aagctactta ggttaagaat
     9901 tcgtgtcgtc cgtgacgaac gagtgacgac ctgaggtcgt aggaggaagc agcgttgaag
     9961 aacaaactcg gttgggcttt ggcgatattg gtgaccggcg cgctcaccct cggcgcaacg
    10021 ggctgcggca gcgacgacgg cggtggcggc ggcggagaca cggcgaagag cagttgcgaa
    10081 accttctgca acaaggagaa ggactgcaac accaacacca ccgtcgccga ctgcatcatg
    10141 tactcctgca gcgatatcga gaaggcctcg gccggctgcc agagtgctct caaagccttt
    10201 tacgattgca tgaacaaggc gaccaacgtt tgcgactccg ctagctgcgc cgcacaagaa
    10261 accgcctatc acgacgcctg ctgaagcgag caggagtgac cgtagagcaa tgacgccgca
    10321 ccgacccttg ctacgcagcc acgctcacgg gcttccgaaa ggcctgcttt gcgcgggcat
    10381 tttcgtccac ctcgccgctc tcgtcttcgc aggctgcgga gggagcggag gttcgtccgg
    10441 cgctggaggc acaggggtcg ggagcggtgg cgcgcccgcc ctcggcggtg cgaattcgag
    10501 cggcggcgtc gcgccgacga gcggcggcac cgcgagcggc ggcacgacga cggcgtccgg
    10561 cggagcttcg actgcttcgg gcggaaagac gaccggaggt ggagcctcga gcggcggtgc
    10621 gtcgtcttcg agcggcggca aaaccgggag cggcggagcc aacaacgccg gcaccagcag
    10681 cgcgcagggc ggcgccacgt cgaccggcgg agtcacctcc aacggcggga cgagcgccgg
    10741 ttgcaccgat ttccaaacgc ccaccaaagt cggcgacatc gccgagaccg cactcgatgg
    10801 cccttcgggc attgccgcga gccgcgcgca cgccggcgtc ctctacgcgc acctcgacgc
    10861 gggcggcggc gccgtcgttt acgcgatgac gtacgcgggc aaggcgctcg gcgcgtacac
    10921 gctgatgggg atcaccgcga ccgactggga agacatcgcg gtcggccctt gcgcgaacgg
    10981 cgattgtatc tacatcggcg acatcggcga caacgcggcg cgaatgggcg gcacgacgcg
    11041 cacgcagatt caggtcctga gagtggcgga gcccaacgtg tccgcgactc aagcggcgac
    11101 gcagcaaatg ctcggcgacg tcacgatcat gcgcttcacg tatcccgaca aagcgcacga
    11161 cgccgagacg ctgatggtcg atccggtcac caaagacatc ctcatcatca ccaaagagac
    11221 cgacggcaac tccagcatct tccgcgccgc ggcgaacacc cctgccgata tgccgaccgt
    11281 gctcgaaaag gtcggaaacg tgcaaatcgg tgcctcgggg caggccgcgc aggcgagcgc
    11341 aggcgacatc tccccgacgg gtgatcggtt catcgtgagg agttacacgg cgatcctgct
    11401 ctggccgcgc gccgcgactt tcgccgcgac gttcgccgcc acgccgaaaa tggtgccgtc
    11461 tccgaccgag acgcagggcg agggactcac cttcacgtcg gacggaaaag cgtggctttc
    11521 cgctggtgaa cgctcgcgcg ccatttacga aggcgttgca agctgtccct gagcggaagg
    11581 gattacgtca cccggcccgc ggctccccgt cacatatgta acgaccgcgt cacctgatcg
    11641 tcacactcgg agtcgacagt aaccccgtcg aaacgaggtt gcacggtcgt gacgcatttc
    11701 gaagaaaccc tgcagcgtga cgtcgagcgg atccgctcga aggttcgcga gatggctgcg
    11761 ctcgccgagg gctcgctgaa cagctgcatc gccgcgctcg tgcagcggaa ccggcaaatc
    11821 gcctactcgg tcatcttgcg cgaccagcgc atcgacgagc tcgacaagga gatcgaccgg
    11881 ctttgcctgg agttcatcct gcgccagcag ccggtcgcgg gcacgctgcg cttcgtctac
    11941 gcggcgatca agatcaactc ggatctggag cgagtcggcg actacgcgga gagcatggcg
    12001 cggcaggggc tctcgctctt cgcgctcgag gtcgacgtgc cggtcgatcg cgtgcaggaa
    12061 atcgccaaca aggcgatccc catgctgcat ggcgcggtcg aggcgttcgt ctctcaagac
    12121 gccgacctcg ccgtgaagac gatggagacc gaagaggtcg tcgatcggtt gcgacatcag
    12181 ctcagccaag acttggtcgg cctgattcag cgccaaaaga tgccgctcga ggcgctcgcg
    12241 ccgctcatga acatcgtgaa ccgactcgag cgcgtggcag atcaagcgag gaacatctgc
    12301 caagagacgc tctacatgtg caccggcgag tacatgaagc acgccggcag cgagacgttc
    12361 cgcctgctgt tcgtcgacga atcgaactct tgcgtgagtc agatggcgga ggcgctcggt
    12421 agcatccagc aggttccgaa cttcgtcttc tcgagcgcgg gcctatcgcg caaacccgta
    12481 gacccggcca cggtgctctt cttgcgcgac aagggcatcg acatttcgcg ctccgtctcg
    12541 aagggcatcg accagatccc gcacctcgat cactaccaaa tcatcgtggc actcacgaag
    12601 gaagcgcagc gcgtatttcc tccgccgccc acgaaagccg tctgcctcga ctggagcctc
    12661 gcggaccctt ctcaatccac agggacgcca gaacaggttc gcgcggctta cgacgaaacc
    12721 tacaagtttt tgaagaccca cattcgcgac ctggtccaag ccatcctcgg cgaccagcac
    12781 aactgaagga aatctcactg atgaagactt acgtaggcac gctgcgcttc ggctgcatcg
    12841 ccctgttcgc gctcggcgtg ttgagcgcct cgtgccgcaa gaagaccgag ccgagcgagg
    12901 ctcaaccggc agggagcgca gaaagcgcag tcgcggccga taacaagaag gagtcgattc
    12961 agaacatcgg ctccgacacg atggtgaacc tcgctcaggc gtgggccgaa gagtacgcga
    13021 aggtcgcccc caacgtgtcg atcgaggtat cgggcggagg ctctggcgtc ggcatcgccg
    13081 ccctcatcaa cggcaccgcc agcgtcgcga acgcgagccg caagctcgag ccggaagagg
    13141 ccgagaaggc caagaaagcg tccggcaaag agccgaagga gttcatggtc ggctacgacg
    13201 gcctcgccat ctacgtgagc aagcagaacc ccctcgagga gatctcactc gaagatctat
    13261 ccgagatcta caaagagggc ggcaagatca acaaatggtc cgagctcggc gtgaagagca
    13321 tccccggcgc caagagtgac gacatcgtgc gggtgagccg acagaacaac tccggcacgt
    13381 accactactt ccgcgaagtc gtgatcggca aaaaagccga ttacaagccc ggttcgctcg
    13441 acatgaacgg ctcgaaggac gtcgtcgagc tcgtcgccaa gactccggga gcgattggtt
    13501 acagcgggct cggctacgcc actcccgacg tgaagatcgt caaggtctcc aagaagaagg
    13561 gcgaacccgc gatcaccccg agcatcgcga cggtgctcga caagacttac ccgatctcgc
    13621 gtccgctctt catgtacacc cccggcgagc ccagcccggc ggttcacaag tacctcgact
    13681 ggatcatgtc cgacgccggg cagaagatcg tcgagcacac cgggtacgtc ccgctcgcaa
    13741 agaagtaagc cacgcgccac cccgaagagg atcccatgaa acacgaagca gccctgctcg
    13801 gccccgcagg tcgcgcccgc acgagcctcg cgccggcaca gctcgcgcag aagcggcgta
    13861 aggtcgaggc cgtaacggag cgaatcctcg aagcgttgat tcgcttgtgc ggagtgagcg
    13921 cgatcctgtt cgtgttcggg atcttcttct tcgtgctacg cgaaggcatc gggttgttgt
    13981 tcgacccggc gttcagcctc agaaagttcc ttttcagcac cgaatggtat ccgacctcgc
    14041 tcagcaacgt gcgctacggc gtgctgtcgc tcatcgtcgg cacggccagc gtcaccgtga
    14101 cggcgatgct gatcgcggtg ccgttcggga tcggcgcggc cgtgttcgtt tccgagttct
    14161 gtagccgccg cgtgaaggag accctcaagg tcgtcatcga gctgctcgcg gcgatcccgt
    14221 cggtggtgtg gggcttcatc ggcatcagcg tgatgaaccc actgattcaa gacgtgtttc
    14281 acgtcccgat cgggctcaac gtgctgaacg gcgcgctctt gctggcgctc atgagcgtgc
    14341 cgatcatggt gagcatcggc gaagacgcct tgaaggcagt gccggactcg ttccgtgagg
    14401 ccgcgatcgc cctaggcgcg acgcgctggc aggtcgttta caaggtgctg ttgccggcgg
    14461 cgaagaacgg cctcttggca gccgcgctac tcggcgtcgg acgcgccgtc ggcgagacca
    14521 tggccgtcct gatggcgact ggacacgcaa tcaacatccc gcacagcgtg ttcgacccgg
    14581 tgcgaacgct caccgcgacg atcgcggcgg agctcggcga ggcttcggtt ggctcggcgc
    14641 actatcaagt gctgttcctg gtcggcatcc tcctcttctc gatcacgttc gtcgtcaacc
    14701 tcaccgccga tctcatcgtc aaaggcgtga agaaaggctg aggcaatgtt cacggcgact
    14761 cccctcgtcc acaagaagca gcgcgccgag ctcgcggcga aggtcctgtt cggggccatg
    14821 gcgctcgcga tggtgatccc cctcgtcttc atcgtgggct acctcgtgat caaggcggct
    14881 ccccttcttt cctgggagtt tctgacgacc aatccgacgg gcggcatgcg ccacggtggc
    14941 atttggtcgg cgctgctcgg gaccatctac ctcgtcacgg tatcgctcct catcgccgcc
    15001 cccgtcggcg tactcgcggc cgtgtacttg aacgagtacg cgcgcgagag cgtcttcacg
    15061 cgcatcatca acctcgcggt cgtgaacctc gccggcgtgc ccagcatcgt ccacgcgctc
    15121 ttcggcctcg gcgcgttcgt gctcttcgcc ggcatcggcc gctcgatcct cgccgcttcg
    15181 ctcacgctcg cgatcatgac cttgcccgtc atcatcgcga gcaccaagga ggcactctcg
    15241 gcggttccga cctccttccg cgaagcgtgc tggaacgtcg gggcgacgcg ctggcaaacg
    15301 atccgcggca tcgtgctgcc gaactcggtg agcggcatcc tcaccggcgt catcttgcag
    15361 gtctcgcgca ctgccgggga aaccgccccc atcatgttca cgggcgcggt gttcttcaag
    15421 aagatccagg aaggcgaccc tttcgcgtac agcatttggg atcagtgcat ggcgctctcc
    15481 atgcaccttt tcaccataac cacgcaggtc accgacgcac cgaaagcgct cccctacgcg
    15541 acggcggtgg tcctgctcgg ctgcgtgctc agcgtgaacg ccaccgcgat cggagtgcgc
    15601 tcgtaccttc gctcgaggaa gaaatggtag ccccccaggc cccgctgccc gccaagatcc
    15661 gcgtgaacga tctgcgcctc gcctacggcg acaagcaggt gctcaacggc atctcgttcg
    15721 acgtacaaga aaacgagatc ttcgcgatca tcggccctgc tcagtcggga aagtcgtcgc
    15781 tgctccgctg cttgaatcgc acgatcgagt tcgtccccgg cgcgaaagcg cagggcagca
    15841 ttcaaatcga cggggccgag gtgagttcga tccacaacgt ctacgagctg cggcgcaagg
    15901 taggcatggt cgcgccgctg ccggtcgggc ttcccctcag catctacgac aacgtcgcct
    15961 tcgccccgcg cgcggctggc ttccggaaca aggccgacct cgacgagctc gtgcaacgct
    16021 gcctcacgca ggccgcgctc tgggacgaag tcaaagatcg cctgaacagc ctcggcacca
    16081 agctctccgg cgggcagcaa cagcgcctga ccatcgcccg cgctctctcg catcagcccg
    16141 agatcctctg cctcgacgag ttctcgatcg ctatcgaccc cgtcaccacg atgcgcatcg
    16201 aggccgtgct gaaggagctc aagcgcgaga tgaccatcgt cctggtcact aacctcgtac
    16261 agcaagcgcg gcgcctcgcg agccgcacca tgttcttgtg gaacggggac atcgtcgaaa
    16321 tcgacaagac cgacgtcatc ttctcttcca agccgaagag ccagaagacg tacgactacg
    16381 tgaacggtat cttcggatga aacgagcggc cagcattgcc gaagagagcg cgcaagaagg
    16441 catcgtcacg cgcgcgctga acctttggta cggcgacttc caagcgctga agaacatcga
    16501 cctgaacatc aagtacggcc agatcacgtc gttgatcggg ccgtccggct gcggcaagac
    16561 gacgctgctg cgctgcttca accgggtgaa cgagcgctac ggctacgtgc gcaccaccgg
    16621 cgagatccga atcttgggga agaacatcta cgactccgac gtttcgctca tcgagctgcg
    16681 gaagtcggta ggcatggtgt ttcaacggcc taatccgctc cccatctcga tctacgacaa
    16741 cgtcgccttc ggcatgcgca tccacaacga gaagcccgcg cgcgccgtgc tcgacgaggc
    16801 cgtggagcga gccctcgtcg aggtcggatt gtgggacgac ctcaaggaca agctcgatga
    16861 gcgcgcgacg tcgctgcagc tcgagcagca gcagaagctc tgcatcgccc gccttttgcc
    16921 ggtgaagccc gaggtcattt tgatggacga gccttgttcc gcgctcgacg tcgaagggac
    16981 ccgcgcgatc gaggagctca tgtgggcgct ccgcgggcgc tacacgatcg tgatcgtgac
    17041 gcacaacatg gcccaggcac gacgcgcgag cgacgagtgc atcttcatgc tcctcggcga
    17101 gctcatcgag caccggcgca ccgaggagct cttcctcaac ccgcagaacc cgaagacggc
    17161 ggagtacatc gaaggacgct acggctgacc ctcagccgaa gcgtttgaga ttgcgcgcgc
    17221 gcgccttctc ggcctcttcc tctcgattct tgggctgcgc cttggtctcg agcgaatcga
    17281 ccaggttacg cgtgatctcg gcgatctggg cgacggcttg ctcgaagagc gcgaggttgt
    17341 gaccagtcgg ggtgcgcgtg ccgctgacct tgcgaacgta ttgcagcgcc gcggcgtgaa
    17401 cctcttcgtc ggtcgcgggt ggctcgaagt tgaagagtgg tcggatgttc ctgcacatcg
    17461 ccgaccaccg tgacgctggt cagccgaggc cgcaaacggc gacggctcac cctctcctac
    17521 acgggagcaa ccgcctcggt gcatgagtag aagtttttca ttccagtccg gggagaagga
    17581 attttgcgcc cagcgtgcca agcggggtga tttggagagg tcagcgcgta aaatgagcgg
    17641 tcgtggctca ggcaggcaga ggctctccgg tggtggttcg tagcggtgac gtactcggcg
    17701 ggaagtacaa gctcgaacgc aagctcggcc aaggcgcaat gggcaccgtc tggtcagcca
    17761 ctcacgtcac gttggggcaa cgcgtcgcca tcaagctgat ctcggcggag cacgcgcagt
    17821 ccgcggaagc gcgcgcgcgg ttca
//
LOCUS       DASTKS010000856         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1923484, whole genome shotgun sequence.
ACCESSION   DASTKS010000856 DASTKS010000000
VERSION     DASTKS010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923484"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFQ35_17820"
     CDS             complement(<1..1260)
                     /locus_tag="VFQ35_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9932568.1"
                     /translation="MPVTNATANWVKALYRDSIVPTLCEYIEIPNKSPVFAPNWQELG
                     HMEQATSLLADWARANLPEGAELEVVRLEGRTPVIFIDVPARGASDKGTVMLYGHLDK
                     QPEMTGWREGLGPWTPTLEGDKLYGRGGADDGYALFACLTALNAAQRDGLPHARCVVL
                     IEACEESGSYDLPYYIEHLAPRIGELSLVVCLDSGCANYEQLWSTTSLRGLVLGTLEV
                     ALLREGVHSGDGTGVIAASERVLRILLNRLEDADTGQVRIPELTTSIPPERRAQAERT
                     AAVLGADVYARFPLEDGVAPVTQDPVELILNRTWRPGLAITGAEGWPAIGNAGNVLRP
                     FTKLKLSIRIPPGVDPARAAQAVKSTLERDPPYGAKVSFRDVSSSAGWDAPALAPWLA
                     RCLEESSARHFGKPAMYMGEGGTIPFMG"
     gene            complement(1369..2571)
                     /locus_tag="VFQ35_17825"
     CDS             complement(1369..2571)
                     /locus_tag="VFQ35_17825"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HET9932569.1"
                     /translation="MSELEPTRLALRQRELDAERIRTSPALASRKRERMAASPLGFLR
                     GSAPLFYEILAARPELAEGPDGDGWIVGDAHLENFGAYRVRGSDSDGDEKKPSTFDLN
                     DFDDAVQGPWRWDVLRLTTSLLLAGRELSIAPLRALDLASALVASYVATACVGAPLPP
                     TPEPVRALLEQVRARSKRQFLDARTQGEGERRRFIVGPRYAELPADVRAQVPAAFRAY
                     LESVAPEERPRESERTIVDAAFRIAGTGSLGALRIAVLTLGKSEASAYIFDLKQVGTP
                     SSAPLLPSSALAPAQRALSACRACLEQPPRMLGTTSLGDASLFGRRMLPQEDRLELEG
                     TTSSAMEPLVRYLGALLGRAHRRGAKTLPKTPWSGDEQSRLIERAASLAGLHLATHYA
                     YCVLARSG"
     gene            complement(2594..3133)
                     /locus_tag="VFQ35_17830"
     CDS             complement(2594..3133)
                     /locus_tag="VFQ35_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9932570.1"
                     /translation="MADPTAKLFYNDFYDYIPAKQQGIYNMVKKLKEQGVPIDGIGMQ
                     CHLNLEPSTNSSNQAYYQDVSHLEDAIELYASLGVDVQVTEMDVSLYVPGVMYTQDTF
                     YTLATFTDPIKAKQAQRYREFFELFRKYKGTITGVTLWGIADDNTWLSEFSSGRKDFP
                     LLFDTNHNPKPAYGAVVDF"
     gene            3614..>4097
                     /locus_tag="VFQ35_17835"
     CDS             3614..>4097
                     /locus_tag="VFQ35_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET9932571.1"
                     /translation="MRELHELLLWLCSIPSVTGGEREIADEIAQYLASLATPLVARRH
                     ENSLVVPLTRGASGPSILLVAQLDTTDVASAATPGRDGERVVGAGAADAKSGLALLLA
                     LTAQRPVMTGNVTLVLHAGGEAGFDNSELGLVFAREPELRSSDFALVMNPTSNRLQLG
                     C"
BASE COUNT          671 a         1297 c         1356 g          773 t
ORIGIN      
        1 gcccatgaac gggatcgtgc cgccctcgcc catgtacatc gctggcttcc cgaaatgtcg
       61 ggcagagctc tcttccaggc aacgcgcgag ccacggggcg agcgcgggcg cgtcccatcc
      121 agcgctcgag ctcacgtctc gaaagctcac cttggctccg taaggcgggt cgcgctcgag
      181 tgtgcttttg acggcctgcg ccgctcttgc gggatcgacg cctggtggaa tccgaatgga
      241 gagcttgagc ttcgtgaaag gtcgtaagac gttccccgcg ttgccgatgg ccggccaccc
      301 ctccgctccc gtgatggcga gtcccgggcg ccaggttcgg ttcaagatga gctctaccgg
      361 atcttgcgtg acgggggcaa cgccatcttc gagcggaaaa cgcgcgtaaa cgtcagctcc
      421 gaggacagcc gccgtgcgtt cggcttgcgc tcgcctttcc ggagggatgc tcgtcgtcag
      481 ctcggggatc cgcacttgcc ccgtatcagc gtcttccagg cggttcagca gaatgcgaag
      541 cactcgctcg ctcgcggcga tgacgccggt cccatccccg gagtgtacgc cctcgcggag
      601 cagcgcgact tccaacgtgc cgagcaccaa acctcggagc gaggtcgtcg accagagctg
      661 ctcgtagttg gcgcaccccg agtcgaggca cacgacgagg gagagctctc cgatgcgagg
      721 cgcgagatgc tcgatgtaat agggcaggtc gtagctgcca gactcttcgc aggcttcgat
      781 caatacgacg caacgcgcgt ggggcaggcc atcgcgctgc gcggcgttca acgcggtcaa
      841 gcacgcaaag agggcgtatc cgtcgtcggc gccgccgcgc ccgtagagtt tgtcgccctc
      901 gagcgtcggc gtccatgggc cgagcccctc gcgccaaccg gtcatctccg gctgcttgtc
      961 gaggtgtccg taaagcatca cggtgccctt gtcgctcgcg ccgcgcgctg gcacgtcgat
     1021 gaagatcacc ggcgtgcgcc cttcgaggcg gacgacttcg agctcggccc cttccggcaa
     1081 attcgctcgc gcccaatcag cgagcaacga ggtggcttgt tccatgtggc cgagctcttg
     1141 ccagttcgga gcgaagacgg gggacttgtt ggggatctcg atgtactcgc agagggtcgg
     1201 gacgatggag tcgcggtaga gagcctttac ccaattcgcg gtcgcgttcg tgacgggcat
     1261 ggctccacca tagtccggcc gctgaacgat gtgtcgattg tcccgttgca cagcacaggt
     1321 tccatggaat gcaggccaaa ctttcggttg gctcacgctg ggtggcgctc agccggaacg
     1381 agcgagaacg cagtaagcgt agtgcgtcgc gaggtgcagc ccggcgaggc tcgcggcgcg
     1441 ctcgatgaga cgactctgtt cgtcgccgct ccacggagtt ttcggcagcg tcttcgctcc
     1501 gcgccggtgc gcgcgtccga gcagcgctcc caaatagcga accaagggtt ccatcgcgct
     1561 gctggtggtg ccctcgagct cgagccggtc ttcttgcggg agcatccgcc tgccaaacag
     1621 gcttgcgtcg ccgagcgatg tcgtgccgag catcctcgga ggttgctcga ggcacgcgcg
     1681 acacgcgctg agcgcgcgct gggctggcgc aagagccgaa ctcggcagca gtggggccga
     1741 gctcggcgtg cctacttgct tgaggtcgaa gatgtacgcg ctggcttcgc tctttcccag
     1801 cgtcagcacg gcgattcgca gtgccccgag gctgccggtg cccgcgatgc gaaacgccgc
     1861 atcgacgatg gtgcgttcgc tctctctggg gcgctcttcg ggcgcgacgc tttcgaggta
     1921 cgcgcgaaac gccgccggca cctgagcgcg aacgtcggcc ggtagctcgg cgtaacgcgg
     1981 gccgacgatg aaccggcggc gctcgccctc accttgcgtt cgcgcatcca aaaactgccg
     2041 tttggagcga gctcgcacct gttccaagag ggctcgtacc ggctccggcg tcgggggcag
     2101 cggagcaccg acgcatgccg tcgcgacgta cgaagcgacg agtgcgctcg cgaggtcgag
     2161 ggcacgaagc ggagctatgc tcagttctcg tccggcgagg agtagactcg tcgtcaggcg
     2221 caagacgtcc cagcgccaag gcccttgcac tgcgtcgtcg aagtcgttca gatcgaaggt
     2281 gctgggcttt ttctcgtcgc cgtccgagtc gctgccgcgc acccggtacg ctccgaaatt
     2341 ttcgaggtgc gcgtccccga ctatccagcc gtcgccgtcc ggcccctcgg cgagctctgg
     2401 gcgcgccgcg aggatctcgt aaaagagcgg cgccgagccc ctcagaaatc cgagcgggga
     2461 tgcggccatg cgctctcgct ttcgcgacgc gagcgcgggc gacgtacgga ttctttccgc
     2521 gtccagctct cgctggcgga gtgctagtcg ggttggctcg agctcactca tggccgtgca
     2581 ggggaggcgc agctcagaag tctaccaccg ccccgtaggc tggcttcgga ttatgattcg
     2641 tgtcgaaaag gagcggaaaa tccttgcgcc cagagctgaa ctcggacagc cacgtgttgt
     2701 cgtcggcgat gccccacagg gtgacacccg tgatggtccc cttgtacttc cggaagagct
     2761 cgaagaactc gcggtagcgc tgggcctgct tggctttgat cgggtcggtg aacgtcgcga
     2821 gcgtgtagaa cgtatcttgc gtgtacatga cgcccgggac gtagagcgac acgtccatct
     2881 cggtgacctg tacgtccacc ccgagcgacg catacagctc gatcgcgtct tcgagatgag
     2941 agacgtcttg atagtaggct tgattcgacg aattcgtcga gggctcgagg ttgaggtggc
     3001 actgcatgcc aatgccgtcg attggcacgc cttgctcctt gagcttcttc accatgttgt
     3061 agatgccctg ctgcttcgcg gggatatagt cgtaaaaatc gttgtagaag agcttcgcgg
     3121 tcgggtcggc caccttcctc aggaacgttg acggtgctat cccacggaga tgcccgagcg
     3181 aggctttcga gatcacctgg tcaggaaaat gcccgcagct gatcgcgctc gtggggccct
     3241 tcgcgcggag ctccgccacc gattcgtcag ttgagctgcc gtggactcgc gcccgatttg
     3301 tcgcgcagga tgcggcgcct gctgcatcgc cccctcgatc tcttcgagca ttcccggcat
     3361 gaggtcaggg aagccggcgt tcgctcggtg tgtgcagctt acggacgacc tccgctgcgc
     3421 catcttcggg cacccggaac ggccgctggt ttgccaatcg ctcgtgccgt cgcaggagat
     3481 gtgcggcgat tcgcgcgacg aggcaatggc aaacttgatc gagctcgagc gtctgacgaa
     3541 accgcgtcct cggtcgtaag tggagcggat gcatggcgtg atcaccccga cgtcatggta
     3601 acttcgggga gcgatgcgcg agctgcacga gttgctactt tggctctgtt cgattccctc
     3661 tgttaccggt ggcgaacggg aaatcgccga cgagatagcc cagtacttgg cgtcactcgc
     3721 tacgccgctc gtcgcaaggc gccatgaaaa ttcgttggtg gtccccctga cccgcggtgc
     3781 ttcggggccc agcattctac tcgtggccca gctcgatacg accgatgtcg cgtcagccgc
     3841 tacgccgggg cgcgacggcg agcgtgtggt tggcgctggc gccgcggacg caaagagcgg
     3901 tctcgcgctg ttgctggccc tcaccgcgca gcgtcccgtg atgacgggca acgtgacact
     3961 cgtgctgcat gcgggcggtg aagcgggctt cgacaacagc gagctggggc tcgtcttcgc
     4021 gcgcgaaccc gagcttcgga gcagcgattt cgcgctcgtg atgaacccga cgagcaacag
     4081 acttcagctc ggttgcg
//
LOCUS       DASTKS010000857         5415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1923626, whole genome shotgun sequence.
ACCESSION   DASTKS010000857 DASTKS010000000
VERSION     DASTKS010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923626"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFQ35_17840"
     CDS             complement(<1..834)
                     /locus_tag="VFQ35_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932572.1"
                     /translation="MARFFRRGFAIRAVVCGLLCSGLPFACGLPDYRFVDEATGGSSG
                     VSRPRCATEQDCERYPNTKTCDPTGHCVECFDTPGVATTCPAGQYCNVNRCDIGCSSN
                     AACPMGLTCDVASHRCRGCAADTDCTPGTGCKSGECVAGCTTEAPCPDGFTCCNGSCA
                     AFGTSPLHCGSCGKACSDEQDCINGECGQGSGCKGWLADCDNSPGCEVNLRTDRKHCG
                     DCATDCGALNCAGGSCTELTCASNTADCDGDKTTKCETVLTNSDNCGICHNACSEDHG
                     KA"
     gene            complement(1205..2575)
                     /locus_tag="VFQ35_17845"
     CDS             complement(1205..2575)
                     /locus_tag="VFQ35_17845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9932573.1"
                     /translation="MIRRAVPALLAGSLSFACSKSNPTDAVSSAAPVSQPSPPKAEVS
                     VSPPSTSAEAKPAAKLPSGLNVLLITIDSLRADMPWNGYSRPIAPNLTKLAERSVSYT
                     NAYSASSYTAKSVAAFLSGRYPSTLYRDGYFFAKYPASDTFLAELLAPRGVRTIGFHA
                     HLYFGKGKGLEQGFDEWRLVPGITFDPETDNFVTSDKMTALAIELPSNPKNTEHQFFA
                     WGHYMDPHDQYQKHKESPDFGSKGRDKYDSEVWFSDYWVAKLLEWAENKPWWKNTVLI
                     VSADHGEAFGEHGMYKHAFEVWEVLTRVPLIIAGPGFSPRRIEQRRSHIDLAPTILEL
                     MGQPIPPDMQGKSLVPELLGGQPESREPILVELTEDSHNPYRRGLISGDYKIIDFGRS
                     KYELYDLSHDPGELHDLSKERKDELERMQKLLEEKVRTLPVVEPYGGAKLKSGATARG
                     PVGPGK"
     gene            complement(2572..2859)
                     /locus_tag="VFQ35_17850"
     CDS             complement(2572..2859)
                     /locus_tag="VFQ35_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932574.1"
                     /translation="MSRLRPVLMLSFGVILAVSAAACREKAPTATNAAPSAAPSPPAQ
                     SAAPRARAAAPFVEPAPAPVPEDFEETVDREITGKNLEKELDKLEREMKTP"
     gene            complement(2856..3434)
                     /locus_tag="VFQ35_17855"
     CDS             complement(2856..3434)
                     /locus_tag="VFQ35_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932575.1"
                     /translation="MNRAWFRIVGAALFVASAANAQEPETAPADSGAGREDQREQHKL
                     DVRNRFDQLRKLRAQRQHAQQLKRDIDAELAKENPDRAVLAQKSAEYAEIRADRRRDR
                     LLSIRRRWGTLADRPDFNRELERHARTQARIERLKFVAATERSGAARTRLLERINHLA
                     ELESERYQKTMQALTEQSRAEDASVKAPGAAE"
     gene            complement(3431..4291)
                     /locus_tag="VFQ35_17860"
     CDS             complement(3431..4291)
                     /locus_tag="VFQ35_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932576.1"
                     /translation="MIEHRSVAALCTLAAVPATLIAATAWIWQPSWALGRALVTACLA
                     ALFAALWVLAAGRRRTIRVERGSTLTWETGIESDDPYGVALHQNGVRELVIENNDPAR
                     VLADARRLARETGAVLLGPEWIAGRRTAPRDPSSFPAISAEGLVWPAQLRASRTSVLA
                     GLFVLVLSAGSIRAESEVSLLSAALPGFSVVLALLIGALLAKLRVVVEASPAGLRAER
                     RGVGAPRTLLNLPLESVLDVNAVGHPSHPETHLLIETVDGPVALTCAEETARGVARTW
                     ASSRRASGAA"
     gene            complement(4884..>5415)
                     /locus_tag="VFQ35_17865"
     CDS             complement(4884..>5415)
                     /locus_tag="VFQ35_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932577.1"
                     /translation="RRRYTRCSMFLRGLVPCCVSVWAIVGCSGGSSTSDTPTSPPQVF
                     IDAIFTTQPDSTQGIARPPDRQPLGTVSLPCDGFFAVRLAYENWDPRAPGLCGEAKNC
                     GHAALTLTLGEQTSETMVVASPALFTLTEQPIWAGTGTLQVELRDDFDSPHLVDGAPA
                     RDQIEVPLAPMDCSNP"
BASE COUNT          933 a         1713 c         1765 g         1004 t
ORIGIN      
        1 tgctttccca tggtcctcac tgcaggcgtt gtggcaaatg ccgcagttgt cgctgttcgt
       61 gagcaccgtc tcgcacttgg tggtcttgtc gccgtcgcaa tcggcggtgt tgctcgcgca
      121 cgtgagctcg gtacaagagc caccggcgca gttcaacgcg ccgcaatcgg tggcgcaatc
      181 gccgcagtgc ttgcggtcgg ttcgcaggtt gacctcacag cccgggctat tgtcgcagtc
      241 cgcgagccag cctttgcagc cgctgccctg tccgcactcg ccgttgatgc agtcttgctc
      301 gtcggagcat gccttgccgc agctgccaca atggagcggg ctcgtgccga aggcggcgca
      361 ggacccgttg cagcaggtga agccgtcggg gcaaggggct tcggtggtgc aacccgcaac
      421 gcactcgccg gacttgcagc ccgtacccgg agtgcagtcc gtgtccgccg cgcaccctct
      481 acagcggtgc gaagccacgt cgcatgtgag ccccatcggg cacgccgcgt tggatgaaca
      541 gccgatgtcg caacgattta cattgcaata ttggccagcc gggcacgtcg tcgcgacgcc
      601 gggagtgtcg aagcactcca cgcagtgacc cgtcgggtcg caagtcttgg tgttcggata
      661 gcgctcgcag tcctgttctg tcgcgcagcg tggacgactc actccggaac ttccgccggt
      721 tgcctcatcg acgaagcggt agtcaggcaa cccgcacgcg aaagggagac ccgagcacaa
      781 gagaccacac acgactgcgc gaatcgcgaa gccgcggcgg aagaagcgag ccattgcagt
      841 agcgtacaac aaccaggccg ctctgcgaga gcccgaagca actgaaggcg accgcgattc
      901 ggccgttcat tcgagtatgg atcgagctcg caggcgcgcg cggcgtggca gttgggtgac
      961 cggctgcgtt ctcgccgtga ctgccctggt gacgctaccg gcgttggcag gaggtggcgc
     1021 cccgctcagc gtgcagagtt gcaatacgcg ctgtcaaagc gagcaaaccg actgcgttct
     1081 gcgctgcgac ggcgatatcc cgtgtattca agagtgtcag aaggtcgctg acacgtgcgt
     1141 cgcgcgctgc caagcgcctg ctccaaagcc tgccagctga gcaatcgagc tccttcgggt
     1201 cgggctattt acccggcccg acgggtccgc gcgcggtggc cccgctcttc aacttcgccc
     1261 caccatacgg ctcgaccacc ggtagcgtcc ggaccttctc ctcgaggagc ttttgcatgc
     1321 gctcgagctc gtctttgcgc tccttcgaca gatcgtggag ttcgccgggg tcgtgactca
     1381 agtcgtacag ctcgtacttg gaccgcccaa agtcgatgat cttgtagtca cccgagatga
     1441 gcccgcgccg gtagggattg tggctgtcct cggtgagctc cacgaggatg ggctcacgcg
     1501 actcgggttg cccgccgagg agctctggta cgagcgactt gccctgcata tcgggcggga
     1561 tcggctggcc catcagctcc aagatggtcg gagcgagatc gatgtgcgac cggcgctgtt
     1621 cgatgcggcg cggtgaaaat cccggacccg cgatgatgag cgggacgcgg gtgagcacct
     1681 cccacacctc gaaggcgtgc ttgtacatcc cgtgctcacc gaaagcctcg ccgtggtcgg
     1741 cgctgacgat gagcaccgta tttttccacc aaggcttgtt ttctgcccac tccagcagct
     1801 tcgcgaccca gtagtcggag aaccaaacct cggagtcgta cttgtcgcga cccttgctgc
     1861 cgaagtcggg ggactctttg tgcttttggt attggtcgtg tgggtccatg tagtggcccc
     1921 acgcgaagaa ctggtgctcg gtgttcttcg gattcgatgg gagctcgatc gcgagcgccg
     1981 tcatcttatc gctcgtgacg aagttatccg tctccggatc gaaggtgatg ccgggtacca
     2041 atcgccactc gtcgaagcct tgctcgagcc cttttccttt tccgaagtaa aggtgcgcgt
     2101 gaaacccaat cgtccgaacg ccgcgcggag cgagcagctc ggcgaggaag gtgtcggacg
     2161 ccggatactt ggcgaagaag tatccgtctc ggtagagcgt cgacgggtac cgccccgaaa
     2221 gaaacgcagc gacgctcttc gcggtgtagc tcgaggcgct gtaagcgttc gtgtacgaaa
     2281 ccgagcgctc ggcgagcttg gtgaggttcg gcgcgatcgg tcgtgagtag ccgttccacg
     2341 gcatatccgc gcggaggctg tcgatcgtaa tcaaaaggac gttcagcccc gaaggaagct
     2401 tcgccgcagg cttggcctcg gcactcgtcg acgggggcga cacgctgacc tcggccttcg
     2461 gcgggctcgg ctgggacact ggcgccgcgc tcgacacggc atcggttggg ttgctcttgc
     2521 tgcaagcaaa gctcagcgag ccggcgagca gggcgggcac ggcacgacga atcatggcgt
     2581 tttcatctct cgttcgagct tgtcgagctc tttctcgaga ttcttcccag tgatctcgcg
     2641 gtcgaccgtc tcttcgaagt cctcggggac gggcgcgggc gcgggttcga cgaacggagc
     2701 ggctgctcgc gctcgcggcg ctgcgctctg cgctgggggt gaaggagctg cgctcggtgc
     2761 cgcgttcgtc gccgttggcg ccttttcgcg acacgcggca gccgataccg ccaaaatcac
     2821 gccaaaagag agcataagca ccggacggag cctgctcatt cggcggctcc gggagctttg
     2881 acggaagcgt cctctgcacg actctgttcg gtgagcgcct gcatggtctt ttggtagcgt
     2941 tcgctttcta gctcagcgag gtgattgatg cgctcgagca gccgcgtccg ggccgcgccg
     3001 cttcgctcgg tggcggccac gaacttcaaa cgctcgatgc gtgcctgggt cctcgcgtgc
     3061 cgttccagtt cacggttgaa gtcggggcgg tcggcgagcg tgccccagcg acgacggatc
     3121 gataggagcc gatcccgacg ccggtcggcg cgaatttctg cgtactctgc agatttttga
     3181 gcgagcacgg ctcggtcggg gttctctttt gcgagctcgg cgtcgatatc ccgcttcaac
     3241 tgctgggcat gctgtcgttg agcgcgcagc ttacggagct gatcgaagcg attgcgcacg
     3301 tcgagcttgt gctgttctcg ctgatcttcc cggcctgcgc cgctgtccgc cggcgccgtt
     3361 tcgggttctt gcgcgtttgc cgcgctcgcg acgaatagcg cggcgccgac gatcctgaac
     3421 caagctctat tcatgctgct ccagatgcgc ggcgtgagct cgcccacgta cgcgcgaccc
     3481 ctcgagccgt ctcctcggcg caagtcagcg cgacggggcc atccaccgtc tcgatcaata
     3541 gatgcgtttc ggggtgactc gggtgcccga ccgcgttgac gtcgagcacc gattcgagcg
     3601 gcaaattcaa cagcgttcgc ggcgctccga ccccacgccg ctcggctcga aggcccgccg
     3661 ggctcgcctc gacgacgacg cggagcttcg cgagcagtgc gccgatcaag agcgcgagca
     3721 cgacggagaa gccgggcagg gcggcactga ggagtgacac ttccgactcg gcgcggatcg
     3781 acccagcaga cagcaccaag acgaagagcc cggccaacac cgacgtgcgc gacgcgcgga
     3841 gctgcgcggg ccagacgaga ccctccgccg aaatggcggg gaacgagctc ggatcgcgcg
     3901 gcgcggtgcg gcgacccgct atccattcgg gcccgagcag gacggctccg gtttcgcgag
     3961 cgaggcggcg ggcgtccgcg aggacgcgtg ccggatcgtt gttttcgatg acgagctcgc
     4021 gcacgccgtt ttggtggagc gccaccccgt atggatcgtc gctctcgatc cctgtttccc
     4081 aagtcagcgt gctgccccgc tcgacccgaa tcgtgcgacg acgcccggcg gcgagcaccc
     4141 acagcgctgc aaagagggcc gcgagacaag ccgtcaccag cgcgcgccct aaagcccagc
     4201 tcggctgcca aatccaggcc gtcgccgcga tcagggtcgc cggaacggca gcaagagtgc
     4261 acagcgccgc aacggagcgg tgctcaatca cgacggttcc gtctgaacga atgtgtcgta
     4321 ttctgcgcac ctacttacct cggccgagag cgatcagacg gcgttcttcc gttcgtggct
     4381 tcctccggcc gggatgaccg tccgagccct cgcgctaggc ggtcacttac atcggtttag
     4441 gccgttgtac cagccccgct ccaatgaccc ttcaactgcg tttcgtcact tgtaaagtga
     4501 acagtcattt cgttttcgaa atgagacaaa tttcatttct gaaaccacgc acccggaaag
     4561 gtctttctaa actctgaata tcactgtgtg gcgcacgtgg atcgctctgt gcacatgtgg
     4621 ctaaacagcg gcagcccaag gtgtcccccc cctccgcctg ggctgccgct cgctttttgt
     4681 gcccagcgga cgttcggcag aagccgctag ggggtgtccc aagttcctgc ccgtcgcgag
     4741 agccaagaaa cgcgagggag aagcgcgccc gaggagtgag caccggagga aggctgaagc
     4801 ctttcgagga gcggagcgac gaggccgtgc tttgcagccg caggatcttg gctcgcagca
     4861 gggcggggaa cttgggacac cccctagggg ttcgagcaat ccatcggcgc cagcggaact
     4921 tcgatctggt cccgcgccgg tgcgccgtcg acgaggtgag gggaatcgaa gtcgtcgcgc
     4981 agttcgacct ggagcgtccc cgtgccggcc caaatgggct gctcggtcaa ggtgaacaag
     5041 gccgggctcg cgaccaccat cgtttcgcta gtttgctcgc ctagcgtgag cgtgagcgca
     5101 gcatgaccgc agttcttcgc ttcgccgcag aggccagggg cgcgagggtc ccaattttcg
     5161 tacgcgagtc gcaccgcaaa gaacccatca caagggagag agacggttcc gagcggctga
     5221 cggtctggcg ggcgcgcaat gccctgagtg ctgtccggtt gcgtcgtgaa aatcgcgtcg
     5281 atgaagacct gtggcgggct cgtcggggtg tccgaggtgg aggaaccacc gctacaaccg
     5341 acgatcgccc acacagagac gcagcacgga actagaccac gaaggaacat gctgcagcgc
     5401 gtgtagcgcc gcctg
//
LOCUS       DASTKS010000858         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1253487, whole genome shotgun sequence.
ACCESSION   DASTKS010000858 DASTKS010000000
VERSION     DASTKS010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253487"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFQ35_17870"
     CDS             complement(<1..575)
                     /locus_tag="VFQ35_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9932578.1"
                     /translation="MIQIFGHPASTCTRKVLTVLAETNTPYELNVVDLSKGEHKQPPH
                     VARQPFGQIPVLDDDGFRMFESRAIARYLDEKSGSPLTPKTIRERALMNQWQSVEQSN
                     FSPAAMKFVYHYVFQRPQDQAVLDGANRMVETTLGAISKSLAEAPYLTGNQFTLADIG
                     YMPYIEYTMGSPLRATFEKFPHVMSWWNRVS"
     gene            950..1342
                     /locus_tag="VFQ35_17875"
     CDS             950..1342
                     /locus_tag="VFQ35_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932579.1"
                     /translation="MSIDAFIRKLENCLNELVTLNIVTAVGAAKLDVKSGDASGTKVD
                     VVDGSTKVIWTAINLAQGDITTVIDPEFQGDAGKELREFHKSRETQGLEIIRGNISAL
                     KELVSLLGAAVEHKNTHPAGSPAVAPKV"
     gene            1355..2041
                     /locus_tag="VFQ35_17880"
     CDS             1355..2041
                     /locus_tag="VFQ35_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932580.1"
                     /translation="MDTTRLENLINDLFSLEVNTIVKQGMMATKMPNAAHALIDLAID
                     YSVTLCRLGAENPMPGDSEASYRVFHDIREVASKRARVIQGLSEDKELAKTQMPKALS
                     DRESADVLLLCRIRDTCDQLKQIFAKSPGGEATRFDRGSANDFRPEQLPLTDRQRLVL
                     RKAWELGTEVVVLQTSVTLDGDVVSRIHPDYAEPEKRGYLEAHQRGVDTSIAFWKTLV
                     GIVRSLFGRA"
     gene            2038..2538
                     /locus_tag="VFQ35_17885"
     CDS             2038..2538
                     /locus_tag="VFQ35_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932581.1"
                     /translation="MNARGLIQELLATGAVTVVTTRDGAPLGEKTALDAVTTIQLDGD
                     VVTYIREDAPLDRHFAALRQVRGRLRRFALCWRFASRGAFWGALAAAQYPAQSWALEA
                     SLRGGAAALGAAMLQPEHWLRSAGGVFAWFGLRAVEALGKRAIRRALSGRSAPVFSGR
                     VAHVRV"
BASE COUNT          441 a          794 c          845 g          471 t
ORIGIN      
        1 tcgctcactc ggttccacca gctcatcacg tgcgggaact tctcgaacgt ggcgcgcaac
       61 ggcgagccca tcgtgtactc gatgtacggc atgtagccga tatcggcgag cgtgaattgg
      121 tttccggtca ggtacggagc ctcggcgagc gacttcgaaa tcgcgcccaa ggtcgtttcc
      181 accatccggt tggccccgtc gagcacggct tggtcctgcg gtcgctgaaa cacgtagtga
      241 taaacgaact tcatcgccgc gggtgagaag ttcgattgct ctacactctg ccactggttc
      301 atcagcgcgc gttcacgaat ggtcttcggg gtcagggggc ttccgctctt ctcgtcgagg
      361 tagcgcgcga tcgcccgcga ctcgaacatg cggaagccgt cgtcatcgag caccggaatt
      421 tgaccgaagg gttgtcgagc gacgtgaggc ggctgcttgt gctcgccctt gctcaggtcg
      481 accacgttga gctcgtacgg tgtgttcgtc tccgcgagga cggtcaggac cttgcgggtg
      541 caggtgctag cgggatgtcc gaagatttga atcattggtg atgctccgcg cggaacgtat
      601 cacacggctc tcacgtgaag gcgtccaccg cgctcgagct tcccgaaccg ttccgcgagc
      661 aaagccgatc ggagctcgcc acccgtgccc gcgaagctcg acgactgcgc gctgcgcgaa
      721 acgcgtctcg ctcgtgcgcg cgagcgctgc ctttcgcgcg cgccacgcgt cctggaaacg
      781 cggcagcttc agtcgcgctc ctcggtgacg tggtgcgacc ggccccgaag ctgggcaatt
      841 ctccccgcag atgccgctgg ttttcgtggg cgttgggtca gactcgccac tcgtgtagca
      901 tccttgcgcc tcgagaatgt tcaggggagg cgtgaaaggt cagcgcagca tgagcatcga
      961 cgcgttcatc cgaaagcttg agaattgttt gaatgagctc gtgaccctca acatcgtcac
     1021 ggcggttggg gcggcgaagc tcgacgtcaa gagcggcgac gcgagcggga cgaaggtcga
     1081 cgtggtcgac ggatccacca aagtgatctg gaccgcgatc aacttggccc agggcgacat
     1141 caccaccgtc atcgatcccg agtttcaggg cgatgcgggc aaggagctcc gggagtttca
     1201 caagtcgcgc gagactcagg ggctcgaaat catccgcggc aacatctccg cgttgaagga
     1261 gctcgtcagt ttgctcgggg cggccgtcga gcacaagaac acgcacccgg cgggctcgcc
     1321 cgcggtagcg cccaaagtgt gaggttaggc gggcatggat acgacccgct tggagaacct
     1381 catcaacgac ctcttttccc tggaggtgaa caccatcgta aagcagggga tgatggcgac
     1441 gaagatgccc aacgcggcgc acgcgttgat cgatcttgcc atcgattact cggtgacgct
     1501 gtgtcggctc ggcgccgaaa atcccatgcc cggtgacagc gaggcgagtt accgcgtctt
     1561 ccacgacatc cgcgaggtcg cgtcgaagcg cgcgcgcgtg attcaggggc tgtccgagga
     1621 caaggagctc gcgaagacgc aaatgcccaa ggctctctcc gaccgcgaat ccgcagacgt
     1681 gcttctcttg tgccgcatcc gagacacttg cgaccagctg aagcaaattt tcgcgaagag
     1741 cccgggcggg gaagcgacgc gtttcgaccg cggcagcgcc aacgacttcc ggcccgagca
     1801 gcttccgctc accgatcggc agcggctcgt gttgcgtaag gcgtgggagc tcggcaccga
     1861 ggtggtcgtc ttgcagacct cggtgacgct cgacggcgac gtcgtttcgc gcattcatcc
     1921 ggactacgcc gagccggaaa agcgcggcta cctggaggcg catcaacgcg gggtcgacac
     1981 ttccatcgcg ttttggaaga cgctggtcgg catcgtacgc tccttgttcg gtcgcgcatg
     2041 aacgcgcggg ggctcatcca agagctgctc gcgaccggcg cggtcacggt cgttacgaca
     2101 cgcgacggcg cgcccctcgg cgagaagacc gcgctcgacg cggtcacgac tattcagctc
     2161 gacggcgacg tcgtcaccta cattcgagaa gacgccccgt tggatcggca ctttgcagcc
     2221 ctccgacagg tgcgagggcg gttgcgacgc ttcgcgctct gttggaggtt cgcctcgcgc
     2281 ggcgcgtttt ggggagcgct ggccgcggcg cagtacccgg cacagagctg ggcgctcgaa
     2341 gcgtcgctgc gcggtggcgc tgcggccctg ggcgcggcga tgttgcagcc agagcactgg
     2401 ctgcgctctg ccggcggcgt gttcgcgtgg ttcggcttgc gcgccgtcga ggcgctcgga
     2461 aaacgagcga ttcgacgtgc gctttccggt cgctcagccc cggtgttcag cggtcgcgta
     2521 gcgcacgttc gagtctgagc ctcgagccgt g
//
LOCUS       DASTKS010000859         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1699868, whole genome shotgun sequence.
ACCESSION   DASTKS010000859 DASTKS010000000
VERSION     DASTKS010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699868"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFQ35_17890"
     CDS             <1..301
                     /locus_tag="VFQ35_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932582.1"
                     /translation="TKYYKVEASGGVQGLVDVFSTITTQLVRSCDIDLKTPPDDPKKV
                     NVAIDCNVIKRADDTWTLDTSTDPNKLLLKGATCERIQAAGAKRVDVIYGCPSVL"
     gene            339..1616
                     /locus_tag="VFQ35_17895"
     CDS             339..1616
                     /locus_tag="VFQ35_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9932583.1"
                     /translation="MNSISFKPFTMLALLAMTASACSSDEGGGGTFDNLGGSSGANKG
                     GSSNSTGHGGSTGIGLGGSSSTSKGGSTSSSSGGYTSSTAGQASACMGVPADLQQQES
                     CTGIEQEAERIPIDMFIMMDRSCSMNYCLGTSGENCSVIPDCSASGGSRWDAIRAGLV
                     DFVEKVKGQDVRAGIGFFGASGVGEDSLDCDVTQYSTPKVPIGAIATTGPQIVDAIDS
                     TPPAGFTPTYPALDGALRYAKSWAMKNPGRRTVVVLVTDGYPTQCSPTTISGIAELAK
                     AGYTTSPPVRTYVVGLAAGFNLDQIAQQGGTNAAFNFDKSNVTSDNLVNTLMNISDSK
                     IACNYEIPPAPTGMVFNPDKVQVLYTPAAGMKQELPKLDNISACDRNPNGGWYYDNPA
                     DPKVINVCPCSCANLQAGTVQVSVGCDPYIGIK"
     gene            complement(2126..3406)
                     /locus_tag="VFQ35_17900"
     CDS             complement(2126..3406)
                     /locus_tag="VFQ35_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9932584.1"
                     /translation="MTKYLSILPIGLLAFACGSNTDSGTPSFGDSSGGSAPIDIGSGG
                     KSSTIDLSSGGSTANAGSGNTSGTGNISNPTNLDELEKASCAGWDGEAEQLPSILQFV
                     VDTSGSMDSETDSTNGQTKWERTRTALLSSIDKLPATVGLGMLFYPNMETIQSSQPSD
                     VNECVRLNALIPIDLLGAAGSAQRNRLATGIKGIRPTSFTPTYDAYTSGVDKGLLPSK
                     LPGKRFMVLITDGAPTLSGSCVMPAGSSGNHAVPVDPDPIVEAIKVAHDAKIQTFVIG
                     SPGSEESIDGEDARVAWLSKAARAGGTDKPGCSDSGPNFCHIDLTQSADFGAALADSL
                     AKIAGAAVQCSYELPSPGAGKTLDLNAINVVLTGSNGSKTLIARSEDPNCSEGWKLNG
                     NLVELCSNTCTQAKADTGASLKLMFGCASVTVVK"
     gene            3633..4127
                     /locus_tag="VFQ35_17905"
     CDS             3633..4127
                     /locus_tag="VFQ35_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932585.1"
                     /translation="MSSKYPLGPLPALRSATTDQRKRELALSEAARSSKEHQLFEISE
                     LAEVARAELARADAAARERLESGLARAVDLAWAEDERRARLSALAELEARRARAREEL
                     GRAEAARNEASRALALAEAEQRAVEKHRDAWARQRESARAELADEEAIERWTSEHSVR
                     SGPR"
     gene            4088..>4603
                     /locus_tag="VFQ35_17910"
     CDS             4088..>4603
                     /locus_tag="VFQ35_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932586.1"
                     /translation="MDERTLSKVWAALKRPTRRALIVLGAALLGCSSAMVLYSSRPRP
                     PEARPPNVVSTSSASAPAVSQAIQLEDFSPLPTDPALRNVADALELSDFARAAEALSL
                     AQKAEPRFATPTVTFWLGRLWERAGNAAAARAACESARIPGSLLHDYAELCRARAALS
                     LGDAKTVASELG"
BASE COUNT          823 a         1550 c         1477 g          753 t
ORIGIN      
        1 caccaagtac tacaaggtcg aagccagcgg cggcgtccag ggcctcgtgg acgtgttctc
       61 taccatcacg actcagctcg ttcgcagctg cgacatcgat ctgaagaccc cgccggacga
      121 ccccaagaag gtcaacgtgg cgatcgactg caacgtcatc aagcgcgctg acgacacctg
      181 gacgctcgac accagcacgg atccgaacaa gctgctcttg aagggcgcga cgtgcgagcg
      241 gattcaagcc gcgggtgcga agcgcgtcga cgtcatctat ggttgcccga gcgtgctctg
      301 aagcgagccg tccttcctac tcgagaggcc gaaccgacat gaactcgata tcgttcaagc
      361 cattcacgat gctcgctctg ctcgcaatga ccgccagcgc ttgcagcagc gacgagggcg
      421 gaggcggcac cttcgataac ctcggcggca gctccggcgc caacaagggc ggcagctcga
      481 actcgactgg ccacggcggg tcgaccggca tcggcttggg gggcagctcc tcgacgtcga
      541 agggtgggtc caccagcagc agctccggag gctacaccag ctccacggcg ggccaggcct
      601 ccgcgtgcat gggcgtacct gccgacctgc aacagcaaga gtcgtgcacg ggcatcgagc
      661 aggaagcgga gcgcatccca atcgatatgt tcatcatgat ggaccggtcg tgctcgatga
      721 actactgcct cggcacgagc ggcgagaatt gcagcgtgat tcccgattgc agcgcctcgg
      781 gtggtagccg ttgggatgcc atccgcgcgg gtctggtcga cttcgtcgag aaggtgaaag
      841 ggcaagacgt acgcgccggc atcggcttct tcggcgcgag cggcgtgggc gaagactcgc
      901 tcgattgcga cgtcacccag tactcgacgc cgaaggtacc gatcggcgcg atcgcgacga
      961 cgggcccgca gatcgtcgac gcgatcgact cgacgccccc tgcaggtttc acgccgacgt
     1021 accctgcgct cgacggtgcg ctccgctacg cgaagagctg ggcgatgaag aacccgggcc
     1081 gccgcaccgt ggtcgtgctc gtcaccgacg gctacccgac ccaatgctca ccgaccacca
     1141 tcagcggcat cgccgagctc gcgaaggccg gctacacgac gtcgccgccg gttcgcacct
     1201 acgtcgtcgg gctcgccgct gggttcaacc tcgaccagat cgcgcagcaa ggtgggacga
     1261 acgccgcctt caacttcgac aagagcaacg tcaccagcga caacctggtg aacacgctca
     1321 tgaacatctc ggacagcaag atcgcctgca actacgagat cccgcccgcg ccgacgggaa
     1381 tggtcttcaa ccccgacaag gtgcaggtgc tctacacccc ggcggcgggc atgaagcagg
     1441 agcttccgaa gctcgacaac atctccgcct gtgaccggaa cccgaacggc ggttggtact
     1501 acgacaaccc tgccgatccc aaggtcatca acgtctgccc ctgcagctgc gccaacctgc
     1561 aagccggcac cgttcaagtc tccgtgggct gcgaccccta catcggcatc aagtagtcca
     1621 ccgagcaatc gcacgaggag cggcagcgtt cgcaaagcgc tgccgctttc ttttgcctcc
     1681 ctgcccacat cgccccctca ccaagccccc tcgagcagcc gacgaagtcg ccccctcacc
     1741 aagccccctg cgagcagcga cgaagtcgcg ccctcgccga gcgccctgcg agcagcgacg
     1801 aagtcgcgtc ctcgccgagt cgccccctcg agcagccgac gaagtcgcgc cctcgccgag
     1861 tcgcccctgc gagcagcgac gaagtcgcgc cctcgccgag tcgccctcga tgcagtcgag
     1921 cagcgacgaa gtcgcgccct cgccgagcgc cctcgacgcc tgacgcgtca acttgccccg
     1981 cccgccaagt ttgggttttg gcaaggcttt cgcgagcatc ggagcggaac gtacgaccgt
     2041 acgtgagcac cgaagcgcag cgaaaacgca gccaaaaccc aaaatcggcg ggcgtcctcc
     2101 cccgtcgcca caacgcgtca ggtcgttatt tgacaacggt gacgctggcg cagccgaaca
     2161 tgagcttaag cgatgcgccc gtgtccgcct tggcttgcgt gcacgtgtta ctgcagagct
     2221 cgacgaggtt gccgttgagc ttccaacctt cgctgcagtt cggatcctcg ctgcgggcga
     2281 tgagcgtctt ggacccgttg ctgccggtca gcaccacgtt gatcgcgttc agatcgagcg
     2341 tcttgccggc cccgggcgag ggcagctcgt aggagcactg cacggcagcg ccggcgatct
     2401 tcgcgaggct gtccgcgagg gcggccccga agtcagcgct ctgggtgaga tcgatgtggc
     2461 agaagttggg tccgctgtcg gagcaaccgg gcttgtcggt gccaccggcg cgagcggcct
     2521 tcgagagcca agcgacgcgc gcgtcctcgc cgtcgatgga ctcttcactg cccggtgaac
     2581 cgatcacgaa ggtctggatc ttcgcgtcgt gcgcgacttt gatcgcttcg acgatcggat
     2641 ccggatcgac cggaacggcg tggttgccgc tgctgcccgc gggcatgaca cagctacccg
     2701 acagcgtcgg agcgccatcg gtgatcagca ccatgaagcg cttgcccgga agcttggagg
     2761 gcagcagtcc cttgtccacg cccgacgtgt aagcgtcgta tgttggcgtg aaggacgttg
     2821 ggcgaatgcc tttgatgccg gtcgcgagtc gattgcgctg cgcggatcct gcggcgccca
     2881 ataggtcgat tggaatgagc gcgttcagtc ggacgcactc gttcacgtca ctcggctggg
     2941 agctctggat cgtctccatg ttcgggtaga acaacatgcc gaggccgacg gtcgctggca
     3001 gcttgtcgat ggacgagagc aacgccgtgc gagtgcgttc ccacttggtc tgtccgttcg
     3061 tcgaatcggt ctccgaatcc atggacccac tggtgtcgac cacgaactgg agaatcgagg
     3121 gcagctgctc ggcttcgccg tcccagccag cgcacgacgc cttctcgagc tcgtcgaggt
     3181 tcgtcgggtt gctgatgttc cccgtcccgc tcgtgttgcc gctgcccgcg ttcgccgtgc
     3241 tcccgcccga ggaaaggtcg atggtggagc tcttcccacc actgccgatg tcgatcggcg
     3301 cgctgccgcc cgaggagtcg ccgaacgagg gggtgccgga atccgtattg ctaccgcagg
     3361 caaatgcgag cagcccgatc ggcaggatcg ataggtattt cgtcatgttt tgagttgctt
     3421 tcgtaaccga ggcctagcgc cgttgcgcgc ggcgtggccc ggaggagttg ccctggatag
     3481 gtcccctcaa tacggaccgc ggaggcaaaa atttgccccc gggccgacac tttgtgctca
     3541 gggagaaact tggtgatatc aacgcaaaca ctgcgtcaga gtgcgctggg gcgaacgtga
     3601 cgttcccggc gcctcgggct aattgaggct tggtgagctc gaagtacccg ctcggcccgc
     3661 ttccagcgtt gcgtagcgcg accacggatc aacgcaagcg cgagctcgct ctttccgaag
     3721 ccgctcggtc gagcaaagaa catcaactgt tcgaaatcag tgagctcgcc gaggttgcgc
     3781 gcgccgagct cgctcgcgcc gacgcggccg cgcgcgagcg gctcgagtca gggctcgcgc
     3841 gcgcggtcga tctcgcgtgg gccgaagacg aacggcgcgc gcgtttgagc gctctcgccg
     3901 agctcgaagc gcgccgagcg cgcgctcgcg aagagctcgg tcgtgccgag gcggcgcgca
     3961 acgaggcgag tcgggcgctc gctcttgccg aggctgagca acgcgccgtc gagaagcatc
     4021 gcgacgcatg ggcacggcag cgcgaaagcg cccgcgcaga gctcgccgac gaagaagcga
     4081 tagagcgatg gacgagcgaa cactcagtaa ggtctgggcc gcgttgaaac gaccgacccg
     4141 gcgcgcgctc atcgtactcg gggcggcgtt gctcggctgc tcttcggcga tggtgctcta
     4201 ctcgagccgg ccgcggccac ccgaggcgcg tccgcccaat gtcgtatcga ccagcagcgc
     4261 ctccgcgccg gccgtaagcc aagcgatcca gctcgaagac ttttccccgt tgccaaccga
     4321 ccccgcgctg aggaacgtgg ctgatgcgct cgagctctcg gacttcgcgc gcgcagcgga
     4381 ggcgctctcg ctcgcgcaaa aggccgagcc gcgtttcgcg acacccaccg tgacgttttg
     4441 gctcggtagg ctctgggagc gcgcagggaa cgctgcggcc gcgagagccg cctgcgagtc
     4501 ggcgcgcatt ccgggctcgc tcctgcacga ctacgccgag ctctgtcggg cacgcgcggc
     4561 cctgtcgctc ggtgacgcca agaccgtagc ctccgagctc ggc
//
LOCUS       DASTKS010000860         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1254462, whole genome shotgun sequence.
ACCESSION   DASTKS010000860 DASTKS010000000
VERSION     DASTKS010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254462"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFQ35_17915"
     CDS             <1..271
                     /locus_tag="VFQ35_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932587.1"
                     /translation="MGCYPFVQHPFGNGCDVETYGSVCLPSGSGTQGALCGESGVDCA
                     AGYVCVIGAHAGRRCAKLCSFSGPNECPLGMICGDTDIQGYGVCD"
     gene            312..1958
                     /locus_tag="VFQ35_17920"
     CDS             312..1958
                     /locus_tag="VFQ35_17920"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HET9932588.1"
                     /translation="MRTLLHNLAGSGENSTSPRPHCLLAAYAAATLVLSVGCGSRSEL
                     GIATGAAGSAGAGGGGPTPSCTVDAECPAVNACGSYRCMAQRCEFEAVSCDDRDPCTE
                     DTCDPGTGCRHRFLSLDMDGDGHRSPRPGFSANNDVCGDDCDDTNPNAFKNNPEACDG
                     IDNDCDGVVDNGFSYLRPQTDAVRVAPPGARRSQRDDGGITATPNAFVIAYTTLADRY
                     ESRLKGVSSSGKTLFETQVSDVNPNTYAGSLTWSGQDLATAWSDARQGGEYDLYARLF
                     GPRGEKQKLPTRITDSPGFSLHPTIKWNQSEYLVAFDDRRAESNAFGDHAQIFGQRLA
                     PDGSLLGGNVLLVDEAGSAEYPSLALSPERVGLAYVFTGDNDGQVRLGLRVLDAALKG
                     VGRAAPVGSDVDTLTLQFVQNRFIALWDVSDGSMQGDAIWGAAFDLSGNVVVQPKRVT
                     TGARFARSVSALSLGDRFLMMWGDDAMTATGVYELYFQTLDADLNVLDARRRFTFSNT
                     TSLSPAMSPGPDASIGVVYESWQDGSRQVYFSTLVCAPTK"
     gene            complement(1966..2895)
                     /locus_tag="VFQ35_17925"
     CDS             complement(1966..2895)
                     /locus_tag="VFQ35_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET9932589.1"
                     /translation="MLEQLPAEVSIYEVSPRDGLQNEAAPIPLTGKRELVQALIRSGL
                     KRIELTSFVSPRWVPQLADAEELASSMQAPPGVVLSALCPNAKGLERARAVGLREVAV
                     FLSASETHNRKNTNKSIDQSLDAFEEVVPAARAAGMQVRAYVSTVWGCPYEGDVDPAV
                     SLRLTQRLLELGCYQVSLGDTIGVGTPLQTRQLLKTFLAEVPSPKLALHFHDTRGTAL
                     ANALMGLELGIADFDASVAGVGGCPYAPGAAGNLATEDLVYMLDGMGVRTGVDLDLLI
                     EAGRVAERVIGRRLPGKVHQAGPRILRPSMRPQ"
     gene            complement(3026..>3810)
                     /locus_tag="VFQ35_17930"
     CDS             complement(3026..>3810)
                     /locus_tag="VFQ35_17930"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET9932590.1"
                     /translation="NVKRSMCIFEYVYFARPDSTLDGISVYEARKNLGRRLSLVQPVE
                     ADVVIPVPDSGVPATIGFSEASGIPFELGLMRSHYVGRTFIEPQQSIRHFGVRLKLNP
                     ISSVLRGKRVVIVDDSIVRGTTSRKIVKMVRAAGAREVHMRISSPPTKWPCYYGIDTP
                     TRAELIASTHSVEEINQYITSDTLAYLPLEAMHEAVVAARATNGAGGQPASDVHGENG
                     RGRQLPVVQNQLDSFCHACWTGQYPVEFTSPPKQRQMRLLDI"
BASE COUNT          630 a         1244 c         1275 g          661 t
ORIGIN      
        1 gatgggctgt tacccattcg tgcaacatcc ttttggtaac ggctgcgacg tcgagaccta
       61 cgggtcggtt tgtctgccat cggggagcgg cacgcaagga gctctgtgcg gagagagcgg
      121 cgtcgactgc gctgctggct acgtctgcgt catcggtgcg cacgcgggca ggcgctgcgc
      181 taagctctgc tcgttcagcg gcccgaacga gtgccccctg ggaatgattt gtggggacac
      241 cgacattcaa ggatacggtg tctgcgactg aaccgggggc actgattcgg cgcgttggac
      301 gagccaagcg aatgcgaaca ttgctccaca acctcgccgg gtccggagaa aattcgacga
      361 gcccgcgacc gcactgcctt ctagccgcgt acgcggcggc gacgctcgtc ctctccgtcg
      421 gctgcggtag ccgctccgag ctcggcatcg caacgggcgc ggctggcagc gctggagccg
      481 gcggcggcgg tcctacgccg agctgcaccg tcgacgctga gtgcccggcc gtgaatgctt
      541 gcggcagcta tcgttgtatg gcgcagcgct gcgagttcga agcagtatcc tgcgacgatc
      601 gcgatccgtg caccgaagac acctgtgatc cgggtacggg gtgtcgtcac cgctttctct
      661 cgctcgacat ggacggcgat ggccaccgca gccctcgccc cggcttctcg gcaaacaacg
      721 acgtctgtgg cgacgactgc gacgacacga atccgaacgc gttcaagaac aacccagagg
      781 catgcgacgg catcgacaac gactgcgacg gcgtcgtcga caacggcttc tcctacctgc
      841 gcccccaaac cgatgcggtg cgcgtcgctc cgccgggagc gcgtcgctcg cagcgggacg
      901 acggcggcat cacggcgacg ccgaacgcgt tcgtcatcgc gtacacgacc ctcgcggatc
      961 gctacgaaag ccggttgaag ggcgtgagct cgagcgggaa gacgctgttc gaaacgcagg
     1021 tctccgacgt gaacccgaat acttacgcgg ggtctttgac gtggtcgggc caggatctcg
     1081 cgaccgcgtg gagcgacgcg cgtcagggtg gagaatacga tctttatgcg cggctcttcg
     1141 ggccccgtgg agaaaagcag aagctgccga cccgcatcac cgactctccc gggttctcac
     1201 tgcaccccac catcaagtgg aaccagtccg agtacctcgt cgcgttcgac gatcgacgcg
     1261 cggagagcaa cgcgttcggc gaccacgcgc aaatcttcgg gcaacgtctc gcgccggacg
     1321 gctcgcttct cggcggtaat gtgttgctcg tcgacgaagc aggtagcgcc gagtatccct
     1381 cgctcgcgtt gagcccggag cgcgtcgggc ttgcctacgt gttcacgggc gacaatgatg
     1441 gccaggtgcg cctcggccta cgcgtgctcg acgccgcact gaaaggcgtc ggacgcgcgg
     1501 cgccggtcgg cagcgacgtc gacacgctca ccctgcagtt cgtacagaac cgcttcatcg
     1561 ccctgtggga cgtctcggac ggcagcatgc agggcgacgc gatttgggga gcggcgttcg
     1621 atctgtctgg gaacgtcgtc gttcagccga agcgggtcac gacgggcgct cgcttcgcgc
     1681 gctcggtgtc cgcgctgtcg ctcggcgacc ggttcctgat gatgtggggc gacgacgcga
     1741 tgacggcgac cggcgtctac gagctctact tccaaacgct ggacgccgat ctcaacgtgc
     1801 tcgacgcgcg gcgtcgcttc accttttcga acacgacttc gctttccccc gcgatgtcac
     1861 ccgggcctga tgcctcgatt ggcgtcgtct acgagagctg gcaagacggt tcgcgccagg
     1921 tgtacttctc gacgctcgtc tgcgcgccga ccaagtagcg cgcgctcatt gcggtcgcat
     1981 cgagggccgc aatatgcgcg ggcccgcctg gtgcaccttg cccggaaggc gtctgccgat
     2041 gacacgctcg gcgacccggc ccgcttcgat caacaaatcg agatccactc ccgtgcgcac
     2101 gcccatcccg tccagcatgt agacgagatc ctcggtggca aggtttccag ccgctccggg
     2161 tgcgtagggg cagccgccga cccctgcgac cgaagcgtcg aaatcggcga tcccgagctc
     2221 gaggcccatg agcgcgttgg cgagcgcggt gccgcgcgta tcgtggaaat gcaaggcgag
     2281 cttcgggctc ggcacttcgg cgaggaaagt cttcaaaagc tgccgggtct ggagcggcgt
     2341 cccgacgccg atggtgtcgc cgagcgagac ttggtagcag ccgagctcga gcaggcgctg
     2401 cgttagtcgc agcgaaacgg ccgggtccac gtcgccttcg tacgggcaac cccatacggt
     2461 cgagacgtag gcgcggacct gcatcccagc cgcgcgagcg gcgggcacga cctcttcgaa
     2521 agcatcgagc gactggtcga tgctcttgtt cgtattcttg cgattgtgcg tctcggacgc
     2581 agagagaaag accgcgacct cgcgcagccc gacggcgcgc gcccgctcga gccccttcgc
     2641 gttcgggcag agcgcgctca gcaccacgcc gggcggagct tgcatcgagc tcgcgagctc
     2701 ctcggcgtcg gcgagctgcg gcacccagcg cggcgacacg aagctcgtga gctcgatccg
     2761 cttgagcccc gagcgtatca aggcctgaac cagctcgcgc tttccagtga gcgggatcgg
     2821 cgcggcctcg ttctggaggc cgtcgcgggg cgaaacctcg taaatcgaaa cctcggcagg
     2881 caattgctcg agcaaggtcc tttcagcgta ccacccgccg ccgcgccgcc caaaccgtcc
     2941 gggcgcgctt gggttttcgg cggggttcga cgaccggggg cgctgaggat cttccaggtt
     3001 cgcccggcgg cagcgaagcg cagctctaga tgtcgagcaa gcgcatctgc cgctgcttgg
     3061 gcggcgacgt gaactcgacc gggtactgtc ccgtccagca ggcgtggcag aagctgtcga
     3121 gctggttctg cactaccggc agctgacggc cgcgcccgtt ttcgccgtgg acgtcgctcg
     3181 cgggttggcc tccggcgccg ttcgtcgcgc gcgccgcgac caccgcctcg tgcatcgctt
     3241 cgagcgggag gtaggcgagg gtatcactcg tgatgtactg gttgatctcc tcgacgctgt
     3301 gggtcgaggc gatgagctcg gcgcgcgttg gcgtgtcgat gccatagtag caaggccact
     3361 tggtgggcgg gctcgagatg cgcatgtgca cctcgcgagc gccggcggcg cggaccatct
     3421 tcacgatctt acggctggtg gtcccgcgca cgatgctgtc gtcgacgatg acgacgcgct
     3481 tgccgcggag cacgctgctg atggggttca acttcaggcg caccccgaag tggcgaatgc
     3541 tctgctgcgg ctcgatgaag gtgcgtccca cgtagtggct gcgcatcaac ccgagctcga
     3601 atgggatccc cgaagcttcc gagaagccga tggtcgcggg cacgcccgag tccggcaccg
     3661 gaatcacgac gtccgcttcg actggctgca ccagcgaaag ccttctaccg agattcttgc
     3721 gtgcctcgta gacgctgatg ccgtcgaggg tcgagtcggg gcgagcgaag tacacgtact
     3781 cgaagatgca catgctgcgc ttcacgttgt
//
LOCUS       DASTKS010000861         6039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_138326, whole genome shotgun sequence.
ACCESSION   DASTKS010000861 DASTKS010000000
VERSION     DASTKS010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138326"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFQ35_17935"
     CDS             <1..608
                     /locus_tag="VFQ35_17935"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9932591.1"
                     /translation="LAVLFGNLAEHGAVVKTAGVDASILRFSGPARVYESQEAAVEAI
                     LGGKVQAGDIVVIRYEGPRGGPGMQEMLYPTSYLKSQGLGKACALITDGRFSGGTSGL
                     SIGHASPEAAEGGLIALVREGDRIEIDIPERKLHLAVSDAELAARHSEELARGSAAFT
                     PHNRQRFVSTYLRAYAALATSASQGAVRDVSRLEAMAPKKS"
     gene            complement(619..1842)
                     /locus_tag="VFQ35_17940"
     CDS             complement(619..1842)
                     /locus_tag="VFQ35_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932592.1"
                     /translation="MTKGFWYRLMHRWFIEYNPLYLVSAACVLVGVNQLSKALGASAH
                     RMLAVAGVADLYAWALVASAALLTRIGLKRPAVMLAFITALYQCDPTLHTETCAYLGT
                     TGFVASLLWLGSFVAKLYALAWALELHGSKSAFALPIVGASGLSLLPFAVQRLSLHGA
                     SALVALWVFGTFAAALWTSRRVASKKWLSLWGRTVLRRSLASVWALWAILLLAHVCFW
                     VREFNLEVTPFLPIPLLLSLRWAERERAAWIAALAALCLAWLSPTTFSLTAGMASVAL
                     VLRALRQSPSTHERTRLFAGSVYGLYLCAWTVSWSTGSWPQHQLALDALLCTALGVMA
                     WRLRPRAAAAVPLVATLAHLGVELGIIVAPKTQLTWGLALVSVGFCLLVGSLVVSVLM
                     RRLPPPPCTEAAEDD"
     gene            complement(2050..5226)
                     /locus_tag="VFQ35_17945"
     CDS             complement(2050..5226)
                     /locus_tag="VFQ35_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HET9932593.1"
                     /translation="MSPKHSIEVLSYWSALLKYQEALSARPRARRVEQSAERLPNLQQ
                     PAPGRDYVKLPFEGASEFFAGSGKFEPLPFDAERTEFFENWLVQRYRRGDADESELGE
                     LVLFPAIQLPREELGGVLRFPVELEWWHAAGQFRAPPPEDRAKGRFPDAPTELRLVRP
                     SREPDQVLPFFLDAGLLQRELRVEPEEIDALFARLRRLRDVKASVMIEATCELLERPL
                     DGERVATVPPRESEAAKQRPPSARLVWRLFEAVQKRLQALNGRSRCYPVGLLVSTERV
                     RATYHVQRDLEQAITSLAEDKLEESSPLTSYLAGRSSELAREPCLGRWASAQLTDNQR
                     EALELGLGSAFCAIQGPPGTGKTTLILEAVAHQLVEKARGIAKGGQPPQTFLMVTSTN
                     NRAVDNVIEPLASGGYADFPLALRLGSREVTSTVTARALGQLLAYVERGGDVSEARFE
                     EARKNFSAALERVEAELAPERRRTEFDRANAQRLREIAALEARIHEAERAHADDNPSI
                     DKSLRQLFGQATLTSAQRARYRDDPAAAQREVAALIRELAKLSREAESDDDAAVKRIE
                     LGFRRVLRQRGQLIERWFGAPLVLPLPPAATAGTRVGTSEWEEGLEQSIGALLSLEAA
                     VGVLASYSADCVRLEQLRAEAASPPQPANDTVVLEPMNDVLAALTQNALELRELWLRR
                     NRTEIRDGLRAAIAACTRGRSLRMLLDASVGPGLWLRRLFPCFGCTLLSLGNAFSSER
                     PAFDRVVVDEAGQCHPAYVVSALLRARSALVIGDVHQLEPVIGLGREDERRIFRGLKL
                     KASEAEMQPYRAHDEAFTSAQSLAERAVPGRPSLRDHFRCQAEIARLFDAWCGYGLVP
                     RAPRASCQSLAPELTAPVLFAEVLGEQERYLGSFRNRLEAMEATSWVTRLLAAGVPAG
                     DIAVITPYRGQSEHLLHALRSARVPVQRPRDELAEEASLELFEGTSGAVAVGTVHRFQ
                     GGERRIVLFSTTVTRHESLGFIDDRVHLLNVATSRAKEHLIVIGSAETLKRGRHTRLL
                     VDAAAELAPRATGHESRLFNGRVPPLER"
     gene            complement(5381..5791)
                     /locus_tag="VFQ35_17950"
     CDS             complement(5381..5791)
                     /locus_tag="VFQ35_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9932594.1"
                     /translation="MSESKNIETVNQIYAAFGRGDVAEIFTHIDRDVDWEFGATEHGI
                     PWLTNGKGLDAVRGFFEALRGLEFHDFRVISVMANGPWVVALVSLECTVKSTGRRLRE
                     VCEAHVWRFDADGRVIAMRHAADTWHHARALGLA"
BASE COUNT         1055 a         1968 c         2016 g         1000 t
ORIGIN      
        1 gtctcgcggt gctattcggc aatttggcag agcacggcgc ggtcgtgaag acggctggtg
       61 tcgacgcgag catcctgcgc ttcagcggtc cggcgcgcgt ctacgagagc caagaagccg
      121 cggtcgaagc catcttgggc ggtaaggtgc aggccggcga catcgtcgtg attcgctacg
      181 aaggtccccg tggcgggccg ggcatgcaag agatgctcta cccgaccagc tatttgaaga
      241 gccaggggct cggcaaagcc tgcgcgctca tcaccgacgg gcgcttctcg ggcggcactt
      301 ctgggctctc gattggccat gcatcgcccg aagcggcaga aggcgggctc atcgcgctcg
      361 tgcgcgaggg ggatcgcatc gagatagaca tcccggagcg caagctccac ctcgcggtca
      421 gcgacgccga actcgccgcg cgccattcgg aggagctcgc gcgcggcagc gccgccttca
      481 cgccgcacaa tcgacagcgc ttcgtgagca cttaccttcg cgcgtacgcg gcgctcgcaa
      541 cgagtgcgtc gcagggcgcc gtgcgcgatg tctcgcggct cgaggcgatg gccccgaaga
      601 agtcctgaga gcccaagctc agtcgtcctc cgccgcttcg gtgcaaggcg gcggggggag
      661 ccgacgcatg aggacgctta cgaccaggga gccgacgagt aggcagaacc caacgctcac
      721 gagcgcaagg ccccaggtaa gctgagtctt cggtgctaca atgatgccga gctcgacgcc
      781 gaggtgggcc agcgtcgcga cgagtggtac tgcggccgcg gcgcgagggc gaagcctcca
      841 cgccatgacg cccaatgccg tgcagagcag cgcgtcgagc gcgagttggt gttggggcca
      901 agaccccgta gaccaactca ccgtccacgc gcagagatag aggccgtaga cgctgccggc
      961 aaacagccga gttctctcgt gcgtgctcgg cgattgtcgc agtgcacgaa gcacgagagc
     1021 gactgacgcc atgcccgcag tgagcgagaa ggtcgtaggg gacaaccacg ccaagcacag
     1081 cgcagccaga gccgcaatcc aagctgcccg ttcgcgctct gcccagcgca gcgagagtag
     1141 caacggtatc ggcaagaacg gcgtaacctc gaggttgaat tcgcgaaccc agaagcacac
     1201 gtgcgccagc agcaggatcg cccaaagtgc ccagacgctc gcgaggctgc gacggagtac
     1261 ggtacgcccc cagagactca gccatttttt gctcgcgact cggcgcgagg tccagagcgc
     1321 ggccgcgaac gttccgaata cccagagcgc gacgagcgcg ctcgccccgt gcaaactcag
     1381 gcgctggacc gcgaacggaa gcaggctcaa gcccgacgcg ccgactatgg gtaacgcgaa
     1441 cgccgatttc gagccgtgta gctcgagcgc ccacgcgagg gcatagagtt tggcgacgaa
     1501 actgccgagc cagagcaggc tcgcgacgaa tccagtagtg cccaagtagg cgcacgtttc
     1561 ggtgtggagc gtcggatcgc actgatagag cgcggtgatg aaagcgagca tcaccgcggg
     1621 ccgcttcagc ccaatgcgcg tgagcaacgc ggcgctcgcg acgagcgccc aggcgtagag
     1681 atcggcgacg cccgcgacgg cgagcattcg atgtgcgctc gcgccgagcg ctttggaaag
     1741 ttgattcacc ccgacgagca cgcatgccgc gctcaccaga tacagcgggt tgtactcgat
     1801 gaaccagcgg tgcatgaggc ggtaccagaa gcctttggtc attgagtgcg cgatagagca
     1861 acttcgatgc cgagggatat ttgctggaaa ttgctctgat tcgccgccct tcagttgtca
     1921 cattggcagg tgcctttcgc ggcggtgccg tcgtgtcatc ggacgacgtc gacaccgcgc
     1981 ttcgcagcag tgcgcgcgag tgaagtgccc gcgcttgccg ccgagcgacg tgatgtatcg
     2041 tgcccggtgt taccgttcga gcggcgggac tctgccgttg aagaggcgac tttcgtggcc
     2101 cgttgcgcgc ggcgcgagct ccgccgccgc gtcgacgagc aagcgggtat gacgcccccg
     2161 cttgagtgtc tccgcactgc cgatcacgat gaggtgttct ttggctcgcg aggtcgccac
     2221 gttcagcaag tgcacccgat cgtcgatgaa gccgaggctc tcgtgacggg tgacggtggt
     2281 cgagaacaaa acgatgcgcc tctctccgcc ttggaagcgg tggaccgttc cgaccgcgac
     2341 tgccccactg gtaccttcga aaagctcgag cgacgcttcc tccgcgagct cgtcgcgcgg
     2401 gcgctgcacc gggacgcgcg cgctcctcag ggcatgcaag aggtgctcgg actgccctcg
     2461 gtacggcgtg atcactgcga tgtctcctgc tggaactcca gccgcgagga gacgtgtcac
     2521 ccagctcgtc gcctccatgg cttcgagtcg gttgcgaaaa ctcccgaggt agcgctcttg
     2581 ctctcccagc acttcagcaa acaacactgg cgcggtgagc tcgggcgcca gagactgaca
     2641 cgaggcgcgc ggggcgcgcg ggacgagccc gtagccgcac cacgcgtcga agagacgagc
     2701 gatttcggcc tgacagcgga agtggtcgcg caaggaggga cgccccggta cggcgcgttc
     2761 ggcaaggctt tgcgcggagg tgaaagcctc gtcgtgcgct cgataaggct gcatctcggc
     2821 ttcggaagcc ttgagcttca atccgcgaaa gattcgtcgc tcgtcttcgc ggccgagccc
     2881 gatgacgggt tcgagctgat gcacgtcgcc gatcacgagc gccgagcgcg cgcgcagcaa
     2941 cgccgaaacg acgtacgctg ggtggcactg cccggcctcg tcgacgacga ctctatcgaa
     3001 tgcaggtcgc tcgctgctga atgcgttacc gagcgagagc agcgtgcacc cgaaacaagg
     3061 aaacagccgt cgtagccaca aacccggccc cacgctcgcg tcgagcaaca tgcgcagtga
     3121 ccggccgcgc gtgcaggctg cgatggcggc gcgcaaaccg tcgcggattt cagttcgatt
     3181 gcgacgcagc cagagctcac gcagctcgag tgcattctga gtgagcgcgg cgaggacgtc
     3241 gttcatcggc tcgaggacga cggtgtcatt cgcgggctgg ggcgggctcg ctgcttcggc
     3301 gcgcagctgc tcgagacgca cgcaatcggc cgaataggat gcgagaacgc cgaccgcggc
     3361 ttccagggaa agcagcgcgc cgatactctg ctcgagtccc tcctcccact cggaagtgcc
     3421 gacgcgggtt cccgcggtgg cggcgggtgg caacggcagc accaacggag ccccaaacca
     3481 acgctcgatg agctgccccc gctggcggag gacgcgccgg aacccgagct cgatgcgctt
     3541 gaccgctgca tcgtcatcgc tctccgcttc gcggctcaat ttcgcgagtt cgcgaatcaa
     3601 ggcggccact tcgcgctgag cggcggccgg gtcgtcacgg taccgtgcgc gttgcgccga
     3661 agtgagtgtt gcctgaccga agagttgccg aagcgacttg tcgatgctcg ggttgtcgtc
     3721 cgcgtgcgcg cgctcggctt cgtggatgcg cgcttcgagc gcagcgatct cgcgcaggcg
     3781 ctgcgcgttt gctcggtcga actcggtgcg tctacgctcg ggcgccagct ccgcctcgac
     3841 tcgttcgagg gccgccgaga agttcttacg agcctcttcg aagcgagctt cactcacgtc
     3901 gcctccgcgc tccacgtagg cgaggagttg gccgagagcg cgcgcggtga ccgtgctcgt
     3961 gacctcgcga ctgccgagcc gcagggcgag cgggaagtcg gcgtagcccc ctgacgccaa
     4021 tggctcgatg acgttgtcga ccgcgcgatt gttggtgctc gtcaccatca agaacgtttg
     4081 cggaggctgt ccccctttgg cgatcccccg agccttttcg accagctggt gcgccaccgc
     4141 ctcgaggatc agcgtcgtct ttccggtgcc ggggggccct tgaatcgcgc aaaatgcgga
     4201 gccgagcccg agctccagcg cctcgcgctg attgtccgtc aactgcgcag aagcccaacg
     4261 gccgaggcag ggctcgcgcg cgagctccga ggagcgacct gccaagtagc tcgttagggg
     4321 gctcgactcc tccagcttgt cttcggccaa agacgtgatc gcctgctcga ggtcgcgctg
     4381 cacgtgatac gtcgctcgta cgcgctcggt gctcacgagc agtccgacgg ggtaacagcg
     4441 gctgcggccg ttgagcgctt gaaggcgctt ttgcaccgcc tcgaacagac gccacacgag
     4501 ccgcgcggag ggcggtcgtt gcttcgcggc ttcgctctct ctcggaggta ccgttgcgac
     4561 gcgctctcca tcgagtggtc gctcgagaag ttcgcacgtg gcttcgatca tcaccgacgc
     4621 tttcacgtcg cgcaggcgac gcagtcgagc gaacagggcg tcgatctctt ccggttcgac
     4681 gcggagctct cgttgcagga gcccggcgtc gagaaagaag ggcagcacct ggtctggctc
     4741 ccgactcggc cggacgaggc gcagctcggt tggtgcgtcc ggaaaacggc ccttcgctcg
     4801 atcttcgggg ggaggcgcac ggaactgccc cgccgcgtgc caccactcga gctcgacggg
     4861 gaaacgcaaa acgccgccga gctcctcccg gggtagctga atggcgggga acagcacgag
     4921 ctcgccgagc tcggactcgt ccgcgtcacc tcgccgatac cgctgtacga gccaattctc
     4981 gaaaaactcc gtgcgctccg cgtcgaacgg caacggttcg aacttccctg accccgcgaa
     5041 gaactccgac gcgccttcga acgggagctt cacgtagtca cggcccgggg cgggctgctg
     5101 cagattcgga agccgctcgg cgctctgttc cacgcgccgc gctctcggcc gcgccgacaa
     5161 cgcctcttgg tacttgagca gtgcggacca atagctgagg acttcgattg aatgcttggg
     5221 gctcatcgtc cggctgctgt ccgagccgcg gagcctatag cgcggcgcaa ggtcgagcaa
     5281 atccgagacg actgttcgcc ggagcgtcgg agcgcgggga ccgctgcgag tggcacggct
     5341 ggctgcgcgg ctcgagttgc gctcggagcg gagcgaacga ctacgcgagg cctagagcgc
     5401 gtgcgtggtg ccacgtgtcg gccgcgtgcc gcatcgcgat gacgcgcccg tcggcgtcga
     5461 aacgccacac gtgcgcctca cagacttcgc gtaagcgccg gcccgtactc ttgacggtgc
     5521 actcgagact cacgagagca acgacccagg gtccgttcgc catcacactg atgacgcgaa
     5581 agtcgtggaa ctccaggccg cgcagcgcct cgaagaagcc gcgcacggcg tcgagtccct
     5641 tgccgttggt cagccatgga attccgtgtt cggttgctcc gaactcccag tcgacgtcgc
     5701 ggtcgatgtg cgtgaaaatc tcggcgacgt cgccccgacc gaaggccgcg tagatttggt
     5761 tcaccgtttc gatgttcttg ctttcgctca tgggatctcc gggttggcct ggcgggcgga
     5821 tgcgcccagc cggcttgctc aaactacggt ccgtcctcga tttcagccga taggcacaat
     5881 ggccgatggg tagcgccccc aatgttgctg tacgttggac gggcatggct tcgagccgcc
     5941 tcgccgagct tctcggggga ctttccctcg cgtgcgacat cgtgagcgcc ttcgccccgg
     6001 gcaaggtgct ccggaccgca gtgctctccg tagaactcc
//
LOCUS       DASTKS010000862         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_360806, whole genome shotgun sequence.
ACCESSION   DASTKS010000862 DASTKS010000000
VERSION     DASTKS010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360806"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VFQ35_17955"
     CDS             complement(<1..773)
                     /locus_tag="VFQ35_17955"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932595.1"
                     /translation="MPTLSSNIVKRQIASVHDVEEALARQAVYGGDLVTNLLEITNVR
                     EAELGAVIAESLGLDAAPAGELPPAPERVRRLLPGDVAQRYGLCPLEEVDGRLVVAVS
                     EPLPLEVVSDLEFSLGLPLVQHASTLVRVQQAVARDYGLTIDQRVARVLAKLAGEPDP
                     HPSVPVLEGPDAPAAEESPEGAAHRPATFEPEPMFTEGPAASPPTTRPYVLDPATHAK
                     AQSQEEAPPAPVEPTAAPAAPKPEAAFTTRSAPRAAHFDL"
     gene            complement(941..1963)
                     /gene="recA"
                     /locus_tag="VFQ35_17960"
     CDS             complement(941..1963)
                     /gene="recA"
                     /locus_tag="VFQ35_17960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9932596.1"
                     /translation="MSEDKRKRNDLLREVLANVEKAYGQGAIMRLGESALEPIAVISS
                     GSLALDEALGVGGYPRGRIVEVFGPESSGKTTLTLHAIAAVQAAGGVAAFIDAEHAFD
                     LRYARGIGIDIRKLLVSQPDCGEQALEIAEMLTRSGAVDLVVIDSVAALVPKAEIEGD
                     MGDAHMGLQARLMSQALRKLTAIANKTNTTLIFINQLRQKIGVVFGNPETTPGGNAMK
                     FYASVRLDVRRIGKITVGETLVGNRTRVKVVKNKVAPPFTEAEFDIRWGTGIDVGADL
                     VETALSAGVLEKSGTHFTFGGKSIGQGRDRTRDNLLTAPELLGEVRKATLEVLATQRR
                     PTTNAA"
BASE COUNT          299 a          649 c          665 g          391 t
ORIGIN      
        1 aggtcgaagt gggcggcgcg gggcgcggag cgagtcgtga acgccgcttc gggcttcggg
       61 gcggccggcg ccgccgttgg ctcgaccggc gcaggcggag cctcttcttg gctttgagcc
      121 ttggcgtgcg tggccgggtc gagcacgtag ggtcgagtgg tggggggcga agcggcgggg
      181 ccttcggtga acatcggctc gggttcgaag gtcgccggcc ggtgcgcggc gccttcgggc
      241 gactcctctg ccgcaggcgc gtcgggcccc tcgagcaccg gcaccgacgg atgcggatcc
      301 ggttctcccg ccagcttggc gagcactcga gcgacgcgct ggtcgatggt tagcccgtag
      361 tcacgagcga cggcttgctg gacgcgaacc aacgtgctcg cgtgttgaac caagggcagc
      421 ccgagcgaga actcgagatc gctcacgacc tcgagcggca agggctcact cacggcgacc
      481 acgagtctgc cgtcaacttc ttccaacggg cagaggccat accgttgcgc cacgtctcca
      541 ggcaggaggc gtcgcactcg ctcgggtgcg ggcggcagct caccagcggg cgcggcgtcg
      601 aggccgaggc tctccgcgat gactgcgccg agctcggctt cgcgcacgtt ggtgatctcc
      661 aacaaattgg tgaccaagtc gccgccgtag acggcctgac gagccaacgc ctcctcgacg
      721 tcgtggacgg atgcgatctg gcgtttgacg atgttggagc tgagcgtcgg catggagggg
      781 gccgagggcc gggatccgag cttcgcgaat tttcctggcg tagcgccccc tgtcaacttt
      841 gccgggtagt tctcggctgc tagaaaggac ggctgccgag ccgttcgctt cgttctccgc
      901 ggattcctct ataaaagggc ccggccccct cgcatcgcct tcaagccgcg ttggtggtcg
      961 gccggcgctg ggttgcgagc acctcgagcg tcgccttgcg tacctctccg aggagctcgg
     1021 gggctgtgag caggttgtcc cgggttcggt cacggccttg cccgatgctc ttccctccga
     1081 aggtgaagtg ggtgccgctc ttctcgagta cgcctgcgga cagcgcggtc tcgaccagat
     1141 cggctccgac gtcgatgccc gttccccagc gaatgtcgaa ctcggcttcc gtgaacggag
     1201 gcgcgacttt gttcttcacc actttcactc gcgtgcggtt cccaaccagg gtctcgccga
     1261 cagtgatctt gccgatgcgt cgcacgtcga gccttacgct cgcgtagaac ttcatcgcgt
     1321 tgccacccgg agtagtctct gggttgccga agacgactcc gatcttctgt cggagctggt
     1381 tgatgaagat gagcgtcgtg ttggtcttgt tggcgatcgc ggtgagcttg cgcaacgcct
     1441 ggctcatcaa gcgcgcctgc aagcccatgt gcgcgtcacc catgtcacct tcgatttcgg
     1501 ctttgggcac gagcgccgcg accgagtcga tgaccaccaa atcgactgcg cccgagcgcg
     1561 tgagcatttc cgcgatttcg agcgcttgct cgccgcagtc cggctgcgac accaagagct
     1621 tgcgaatgtc gatgccgatc ccgcgggcgt agcgcaggtc gaaagcgtgc tctgcgtcga
     1681 tgaacgcggc gacgccaccg gcggcttgaa cggccgcgat cgcgtgcagc gtgagcgtcg
     1741 tctttccgct cgactccgga ccaaacactt cgacgatccg tccgcgcgga taacctccga
     1801 cgccgagcgc ttcgtcgagc gccaaagatc cgctcgaaat caccgcgatg ggttcgagcg
     1861 ccgactcacc gagtcgcatg atcgctccct gcccatatgc cttctcgacg ttggctagca
     1921 cctcgcgcaa aaggtcgttg cgcttgcgtt tgtcttcgct cattgctttt tctctccttc
     1981 gttgcccaag cgggcgtgga cgac
//
LOCUS       DASTKS010000863         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1700709, whole genome shotgun sequence.
ACCESSION   DASTKS010000863 DASTKS010000000
VERSION     DASTKS010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700709"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFQ35_17965"
     CDS             <1..850
                     /locus_tag="VFQ35_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9932597.1"
                     /translation="LFVSLGECITERSGRVNLGLEGTLVMGAMSGYGASYLSGSAWLG
                     VLAAGAVGGLFGAVHGTLCARPRVNDIAVGIALMLLGAGLAFFLGKPLIQPSAPRLPA
                     IPLGFWSATPQVRSALDVNALFIVGLGLAPLLRWALKNTRYGLILRTVGESAESARAM
                     GYDVNRVRLAATAVGGVLAGIGGSFLSLFYPGSWNEGLSSGQGLMAVALVIFARWDPV
                     RCLWASLLFGGASALGPALQSVGITQGYYLFNAAPYALTLLIMVLSSSATQTSADMPA
                     ELARAR"
     gene            860..1555
                     /locus_tag="VFQ35_17970"
     CDS             860..1555
                     /locus_tag="VFQ35_17970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET9932598.1"
                     /translation="MAFVASDPYPWPYDGDLRPENTTLIVIDMQTDFCGKGGYIDLLG
                     YDISNSRACIEPISKLLTAFRKRGFHIIHTREGHRPDLADLPANKRWRSRRMQNGAAP
                     QTGIGDVGPCGRVLVRGEPGWDIIPELYPVAGEPIIDKPGKGSFCATDLDLILRQRGI
                     RNIVLTGITTDVCVHTTMREANDRGYECLILGDCCAATDMGNHQAALKMVTMQNGVFG
                     AVSTSKALLEVIG"
     gene            1555..3126
                     /locus_tag="VFQ35_17975"
     CDS             1555..3126
                     /locus_tag="VFQ35_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9932599.1"
                     /translation="MAAPARAIELGPEVSGERLVKRPPLLEAEGIYKHFGPLAALEDV
                     SFRLEPGTFHALLGENGAGKSTLVKCIMGFYHADRGKVLVGGEPLTLKSPREAQRHGI
                     GMVYQHFTLVENMTVAENLVLARADVPRVIDWRKEQAAIDAFIEGMPFKVPVRKLVRQ
                     LAAGEKQKLEIIKQLYLGSRIVILDEPTSVLTPQEADEVLGMLGEMAREGRVSVAMIT
                     HKFREVMQFADSVTVLRRGRVAGTGSKRDLTPEAMAEMMVGSEPPRASLERAALEARE
                     HLLEIRELEALDDIGLPVLRGVSLGVRAGEIVGIAGVSGNGQDELVEVLGGQRGALSG
                     TVHVGGKPYRGTRSELRSERVRLLPEAPLRNACVADMSVAENIGFRSFDVAPFAFLRW
                     LVDHAKVRRHARTAVREFRIKTPSVETPIGALSGGNVQRAVLARELHGEVALLIAANP
                     CFGLDFAAVSEIRSRIMAARNAGAGVLLVSADLEEIFALSDRILVMSEGKIVFETTAA
                     KASVPEIGHHMAGGH"
     gene            3184..3528
                     /locus_tag="VFQ35_17980"
     CDS             3184..3528
                     /locus_tag="VFQ35_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9932600.1"
                     /translation="MDARVFALAALREKTDFKPFRQGVEVSWLYGEGGNGSSAALLRY
                     APGAHVPRHRHGGYEHILVLEGSQCDDAGRYGVGTLIVNEPNTEHEVWSVDGCLVLVI
                     WERRVTFVDSRS"
     gene            3684..>4827
                     /gene="urtA"
                     /locus_tag="VFQ35_17985"
     CDS             3684..>4827
                     /gene="urtA"
                     /locus_tag="VFQ35_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HET9932601.1"
                     /translation="MKTRTATILLSLIGAIAAACKGPGEAAPSAPADSAKAAASVAVA
                     PAAAPAKVDDTGPIKVGILHSLSGTMAISETSLKDVALMTIEKINAAGGVLHRKLEPV
                     VVDPASNWPLFAEKARELIQKEHVAVTFGCWTSVSRKSVLPVFEELNGLLFYPVQYEG
                     EESSYNVFYTGAAPNQQAIPAVEYLMSAKGGGAKKWVLLGTDYVYPRTTNKILNYFLK
                     SKGVTEANIMEKYTPFGHSDYQTIVADIKKFAAGTPTAVVSTINGDSNVPFYKELANQ
                     GLKASDIPVVAFSVGEEELRGIDTKPLVGHLAAWNYFMSIDTPENKKFIADWKDYVKS
                     HNLPDGDKRVTNDPMEATYIGIKMWAQAAEQAGTTDINAVRQAIGYQ"
BASE COUNT          854 a         1529 c         1586 g          858 t
ORIGIN      
        1 cctcttcgtg agcctcggcg agtgcatcac cgagcgctcc ggccgcgtga acttgggcct
       61 cgaagggacg ctcgtgatgg gagcgatgtc cggctacggc gcctcctact tgtccggctc
      121 cgcgtggctc ggcgtcctcg ccgcgggcgc ggtgggcggc ctgttcggtg ccgtgcacgg
      181 gacgctctgc gctcgaccgc gcgtcaacga catcgccgtc ggcatcgcgt tgatgctgct
      241 cggcgcgggc ctcgcatttt tcttgggcaa gccgctcatt caaccgagcg cgccccggct
      301 ccccgcgatt ccgctcggct tttggagcgc gacgccgcag gtgcgttccg cgctcgacgt
      361 gaacgcgctg ttcatcgtcg ggctcgggct ggctccgctc ctgcgctggg cgctcaagaa
      421 cacacgctac ggcttgatct tgcgcacggt gggcgagagc gccgagtcgg cccgcgccat
      481 gggttacgac gtgaatcggg tccgtctcgc cgcgaccgcg gtcggcggcg tgctcgctgg
      541 catcggaggc tcgttcctgt cgctcttcta tccaggcagt tggaacgagg gcctttcgag
      601 cggtcagggg ctgatggcgg tcgcgctcgt catcttcgcg cgttgggatc ccgtgcgttg
      661 cttgtgggcg tcgctcctat tcggcggcgc cagcgcgctc gggcccgcgc tgcagtcagt
      721 cggaatcact caaggctact atctcttcaa cgcagcgcct tatgcgctca cgctgctcat
      781 catggtgttg agcagctcgg ctactcagac gtccgccgac atgcccgcgg agctcgcgcg
      841 agcccgctga ggagcgaaca tggcattcgt tgcatccgac ccttaccctt ggccgtacga
      901 cggcgacttg cggcccgaga acacgaccct gatcgtgatc gatatgcaga ccgacttctg
      961 cggcaagggc ggctacatcg acctcttggg ctacgacatc tcgaatagcc gcgcgtgcat
     1021 cgagcccatt tccaagctgc tcaccgcttt ccgtaagcgc gggttccaca tcatccacac
     1081 gcgcgaaggg caccgccccg atctcgctga tctgcctgcc aacaagcgct ggcgttcacg
     1141 ccgcatgcag aacggcgcgg cgccccagac cggaatcggc gacgtcggtc cgtgcggtcg
     1201 cgtcctggtc cgcggggagc ccggttggga catcatcccc gagctttacc ccgttgccgg
     1261 cgaacccatc atcgacaagc ccggcaaagg gtccttctgc gcgacggacc tcgacctgat
     1321 tttgcgccag cgcggcatcc gcaacatcgt gctcaccggc attaccaccg acgtttgcgt
     1381 gcatacgacc atgcgcgagg cgaacgatcg cggctacgaa tgtctcattc tgggcgactg
     1441 ttgcgcggcc acggacatgg gcaatcacca agctgctctc aagatggtca ccatgcaaaa
     1501 cggtgtgttc ggcgcggtga gcacgtcgaa ggcgctgctc gaggtgatcg gctgatggcg
     1561 gctccggcgc gcgccatcga gctcgggccg gaggtctccg gtgagcgcct cgtcaagagg
     1621 ccgcctttgc tcgaagccga gggcatctac aagcacttcg ggccattggc tgcgctcgag
     1681 gacgtctcgt ttcgcctcga gcccggcact tttcacgcgc tgctcggcga aaacggcgcc
     1741 ggcaagagca cgctcgtgaa gtgcatcatg ggcttttacc acgccgaccg cggtaaagtg
     1801 ctcgtgggcg gcgagccgct cacgctgaag agcccgcggg aggcgcagcg ccacggtatc
     1861 ggcatggtct accagcactt tacgctggtc gagaacatga ccgtggccga aaacctggtg
     1921 ctcgcgcgcg cggacgtgcc gcgcgtcatc gactggcgca aagagcaagc cgcgatcgac
     1981 gccttcatcg agggcatgcc cttcaaggtg ccggtgcgaa agctcgtgcg gcagctcgcg
     2041 gcgggcgaaa agcaaaagct cgagatcatc aagcagctct acctcggcag ccgcatcgtc
     2101 attctcgacg aaccgaccag tgtgctcacg ccgcaagaag cggacgaggt gctcggcatg
     2161 ctgggcgaga tggcgcgcga agggcgcgtc agcgtcgcga tgatcacgca caagttccgc
     2221 gaggtgatgc agttcgccga cagcgtgacc gtgctgaggc gcggccgcgt ggcaggcacc
     2281 ggttctaagc gtgacctcac gcccgaagcc atggccgaga tgatggttgg ctccgagcct
     2341 cctcgtgcct cgctcgagcg cgcagcgctg gaggctcgcg agcacctgct cgagatccgt
     2401 gagctcgagg cgctcgacga catcggtctg cccgtgttgc gtggggtgag cctcggcgtg
     2461 agagcggggg aaatcgtcgg catcgcgggt gtgtcgggca atggccaaga cgagctcgtc
     2521 gaggtgctcg gtggtcaacg cggggcgctc tcgggcaccg ttcacgtcgg aggcaagccg
     2581 taccgcggga cgcggagcga gctccgctcc gagcgagtgc gattgctccc cgaggcgccg
     2641 ctacgcaacg cgtgcgtcgc cgatatgagc gtcgccgaaa acatcgggtt ccgctctttc
     2701 gacgtcgcgc cgttcgcgtt tctgcgttgg ctcgtggatc acgccaaggt gcgtcggcac
     2761 gctcgcacgg ccgttaggga gtttcgcatc aagactcctt cggtggaaac gcccatcggc
     2821 gcgctctcgg gaggaaacgt gcaacgcgcc gtcctcgctc gcgagttgca cggcgaagtc
     2881 gcgctgctca tcgcggcaaa tccctgcttt gggctcgact tcgcggccgt gagcgagatt
     2941 cggagccgaa tcatggcggc gcgcaacgcg ggcgcgggag tcctcctcgt aagcgccgat
     3001 ctcgaagaga tctttgcgct ttcggatcgc atcctggtga tgagcgaggg caagatcgtc
     3061 ttcgagacga cggccgcgaa ggcgagcgtg cccgaaatcg gtcatcacat ggccggcggc
     3121 cattgagggt acgtgcggtg ccgtaacggc ggcattgccg tatagtcccg aggagtcatg
     3181 tcgatggatg ctcgtgtatt tgcgctcgcg gctctccggg aaaagacgga cttcaagccg
     3241 ttccgccaag gcgtagaggt ttcctggctc tacggtgagg gcggtaacgg gtcgtcggca
     3301 gcgctcttgc gctatgcacc gggcgcgcat gttccgcggc atcgacacgg cgggtacgaa
     3361 cacatcttgg tactcgaggg ctcgcagtgt gacgacgcgg ggcgttacgg tgttggcacg
     3421 ttgatcgtga atgaacccaa caccgaacac gaggtgtgga gtgtcgatgg ttgtctcgtg
     3481 ctcgtcattt gggagcgccg cgtcacgttc gtcgattcgc gctcttgagc gaagctgagt
     3541 ggccggcccg agaaaagata tgtgggtgtc gtggcagcga cacaattccg aaacacgtcg
     3601 ccggggatga caaggggcgt tcggaaaccc gcctagagcc tttttcgcgg gccaaacccg
     3661 ggtgcgccga ccaggagaca aaaatgaaga cccgcactgc gacaattttg ttgagcctaa
     3721 ttggagcgat cgccgccgcc tgcaagggcc cgggggaggc tgcgccttcc gctccggccg
     3781 atagcgcgaa ggctgccgcg tccgtcgcgg tggcaccggc agcggcaccc gcgaaggtag
     3841 acgatacggg tccgatcaaa gtcggtattt tgcactcgct gtctggcacg atggcaatca
     3901 gcgagacgtc gctcaaagac gtcgcgctca tgaccatcga gaagatcaac gcggcgggcg
     3961 gcgtgctgca ccgcaagctc gagccggtcg tcgtcgaccc cgcgtcgaac tggccgctct
     4021 tcgccgagaa ggcgcgcgag ctcattcaaa aggagcacgt cgcggtcacg ttcggctgct
     4081 ggacgtcggt gtcgcgcaag agcgtgttgc ccgtgttcga agagctgaat ggcctgctct
     4141 tctacccggt ccagtacgag ggcgaagagt cgtcgtacaa cgtcttttac acgggtgcgg
     4201 cgccgaacca gcaagcgatc cccgccgtcg aatacctgat gagcgcgaag ggcggcggcg
     4261 cgaagaagtg ggtgctgctc ggcaccgact acgtctatcc gcgcaccacc aacaagattc
     4321 tgaactactt cttgaagagc aagggcgtga ccgaagccaa catcatggag aagtacacgc
     4381 cgttcggtca cagcgattat caaaccatcg tggccgacat caagaagttc gcggcgggca
     4441 cgcccacggc ggtggtctcc accatcaacg gcgactcgaa cgtgccgttc tacaaagagc
     4501 tcgcgaatca gggcttgaag gcctcggaca tccccgtggt cgcgttctcc gtcggtgaag
     4561 aagagctccg cggcatcgac acgaagccgc tcgtcggtca cctcgcggcg tggaactact
     4621 tcatgtccat cgacactccc gagaacaaga agttcatcgc ggattggaag gactacgtga
     4681 agtcgcacaa cctgcccgac ggcgacaagc gcgtcaccaa cgacccgatg gaagcaacgt
     4741 acatcggcat caagatgtgg gcgcaagccg cggagcaagc cggcacgacc gacatcaacg
     4801 ccgtacgcca agccatcggt taccaga
//
LOCUS       DASTKS010000864         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1478495, whole genome shotgun sequence.
ACCESSION   DASTKS010000864 DASTKS010000000
VERSION     DASTKS010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478495"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..579
                     /locus_tag="VFQ35_17990"
     CDS             127..579
                     /locus_tag="VFQ35_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932602.1"
                     /translation="MTSVSGVVSELSSFLTLGDLLEELRRRWGAYELVDHWQQGEFHH
                     DLIVRVPTARGAFGGDVLVIATNCNGGVKELLCFEGVPERGALWHSRCPESPEFSGHA
                     PQLVAAYRTEHWFDPCELLSSSARSEYREEFRERQCGGGWMLRKSTAS"
     gene            607..1113
                     /locus_tag="VFQ35_17995"
     CDS             607..1113
                     /locus_tag="VFQ35_17995"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HET9932603.1"
                     /translation="MSMITTFRRLSDEDLERLCDEPGVVEDYLGAEEPIEGFGPFAEL
                     DVDKAWHAIHFMLTGSAWEGDPPLNFIVSGGTEIGEDMGYGPARGLLNMEVRNVAAAL
                     QLLPTAVLMRRFDRAAFAAAEIYPPIWGRSLEEDDTRGYVAENYEALRAFIIDAATAG
                     EGLLIAVR"
     gene            complement(1141..2238)
                     /locus_tag="VFQ35_18000"
     CDS             complement(1141..2238)
                     /locus_tag="VFQ35_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9932604.1"
                     /translation="MTRLLSLACLPLLLALGCARPSEQRAERDLVIGQATAQNVAVRV
                     DQGLAAVREISADAIVLWGSAPTFTFSLEVDRATTTRLELQNTLVDAELVSIATTAPV
                     TLRELAPPVPTRHVFELELPPGVSTFRVAPNDSESEAPFRFALMSDVQEAIDRVSDIF
                     RRLNVEPGVRFLLGAGDLTQRGTLEQTEQYQRELLALDVPYFTTLGNHELGTDPPLYQ
                     DYFGRANFHFVFKGVEFTLLDSGSATIDPMVYDWLDGWLDAARSRVHVVSMHIPPLDP
                     IGVRNGCFASRAEAAKLLGRLAEGQVDLTVYGHIHSYYEFENAGIPAYISGGGGAIPE
                     RFDRIGRHFMVFDVDSENGIRETHRIDIDVN"
     gene            complement(2235..3254)
                     /locus_tag="VFQ35_18005"
     CDS             complement(2235..3254)
                     /locus_tag="VFQ35_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932605.1"
                     /translation="MPGRRALGVTAAVLPGALVHGAGHYVIGERDTAGQLLALEGVGL
                     SMILGGGGVLFASGASRWVVGPAAAIAMMGFGVFGTSLLADVYGTAASDQGAARRPML
                     PKVETEFGYRRLEDVQFSHHDFLVEGMRLNQGSFGLGLLGFFSMGAGNARYRADVLYR
                     FLDDSDVADSRYADRLELDLGFVRHTYPTERFAMTSGEASVLARYDLAHVGRSLRGSF
                     AEFALGYARGAISYDIEGQHVPADSYDLLLGRFGFGVRLRGKSAPGSEALLYYDHRHD
                     DFAAGLKVTGLGSGVAGHFGFQTKWYFNSSFGVLTDVNVGSAWFFGASLLLRQPELQR
                     RVRSP"
     gene            complement(3500..4177)
                     /locus_tag="VFQ35_18010"
     CDS             complement(3500..4177)
                     /locus_tag="VFQ35_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932606.1"
                     /translation="MTAVGLAMWASRITIPYYSAHGTVRNPALDARLAYLLPRALVVL
                     AAFTAAYWVINAWSGHDQSVVDRGETLLFYEKPFAARFWSTFGWALCATAVFVLIACS
                     LLLSWVGKIVLLAFLLPFFGAAVLGIIASNDRLHVDLQRRTVRSRALFRKPAYEISFD
                     ELRMLQVEKQAPRPGTKQMYRVIVKGRELPAPLGTYPSRKEALQFISRLKSVAGAARN
                     LPTRPGA"
     gene            complement(4295..>5639)
                     /locus_tag="VFQ35_18015"
     CDS             complement(4295..>5639)
                     /locus_tag="VFQ35_18015"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HET9932607.1"
                     /translation="NPARTPGGACTKDADCNAGAVCNQKAGTCVCLQSGWKRDTEDDS
                     SKVTSSSVFDFNGDGAAEVVYNDECYFHVYDGATGGLYLALPSLSRTIVENPVVADVD
                     NDGNAEIVFVQNNETIQCSEANLDSWPKGNNDVPATSLPNGLEVWGDPSDVWVAARRV
                     WNEHSYHVTNVTEGGQIPLHEPEHYKPLNGRLYNTYRSQPRNYGVAPDLIPIAIQITS
                     PDVACGQLSNQIEISVLVKNAGDLRVGPGVQIGFDGSWMTPAVTAELLDANGAPITVT
                     LDTSLEPGASTVVSVTYTAGNNGRPDLPVEVKATVDAKQAERECHEDNNQIAAPVEAG
                     AALADLIVAIDKAVGCSPPDATVTVANLGSAEATNILVRIYAGDPSRGGQVIGETTLA
                     GPLAAGDLVRFHVELETIYRNVTLYASVDPLNTVAECNDANNIALGPALNCAVLR"
BASE COUNT          992 a         1758 c         1890 g          999 t
ORIGIN      
        1 gggcgacggg agggatttag gattggctct tagagagtac gtttacatcg cgagcgtagg
       61 gccacgtcgc ggaggctcgt gagctcacgc ctcgctcgcc gaggaccttc gcgctaggct
      121 tctctggtga cgtcggtgag cggtgtggtg agcgaactgt cgagctttct cactctgggc
      181 gacttgctcg aggagcttcg ccgacgctgg ggcgcctacg agctcgtgga ccattggcaa
      241 cagggtgagt tccaccatga cctcatcgtg cgagtgccaa cggcccgcgg cgcctttggc
      301 ggtgatgtct tggtcattgc gacgaactgc aacggcggcg tcaaggaact gctttgcttc
      361 gaaggcgttc cggagcgggg cgcactgtgg catagccggt gtcctgaaag tcccgagttt
      421 agcgggcacg ctccgcagct cgtcgccgcc taccgcaccg aacactggtt cgatccgtgc
      481 gagctcctgt cttcgagcgc gcgcagtgaa taccgcgaag agtttcgcga acggcagtgc
      541 gggggtgggt ggatgcttcg aaaaagcacc gcttcttgat gggggagacg gaaagagagg
      601 cacccgatga gcatgataac gacgttccga cgtctgtccg acgaagacct cgaacgcttg
      661 tgcgacgagc ccggcgtagt ggaggactac cttggcgccg aggagccaat cgagggcttc
      721 ggcccttttg cggagctcga cgtcgacaag gcgtggcatg ccattcactt catgctcacc
      781 gggagcgctt gggagggcga cccaccgctc aacttcatcg tgagtggagg caccgagatc
      841 ggggaggaca tgggctacgg tccggcgcgg ggcctactca acatggaggt gcgcaacgtc
      901 gcagccgcgt tgcagctcct gccgaccgct gttctcatgc gtcgcttcga ccgagccgcg
      961 ttcgcagccg ccgaaattta cccccccatc tggggccggt ccctcgaaga agacgacacg
     1021 cgcggctacg tcgccgagaa ctatgaggcg ttgcgagcct tcatcatcga cgccgcgacg
     1081 gccggagaag ggctgctcat tgcggtccga tgagcgcgcc ttcttcgccg gtcgtcgatg
     1141 tcaattcaca tcgatgtcga ttcggtgagt ttctcggatg ccgttttcgg agtcgacgtc
     1201 gaagaccatg aagtgcctgc cgatgcgatc gaagcgctct gggatcgcac cgccgccgcc
     1261 ggagatgtaa gccggaatgc ccgcattctc gaactcgtag taagagtgga tgtgaccgta
     1321 gaccgtgaga tcgacctggc cttcggcgag gcgaccgagg agctttgccg cttcggcacg
     1381 gctcgcgaag cacccattgc gcacgccgat gggatcgagc ggaggaatgt gcatcgagac
     1441 gacgtgcacg cgtgaacgtg cggcgtcgag ccaaccgtcg agccagtcgt acaccatggg
     1501 atcgatggtc gcgctcccgg agtcgagcaa cgtgaactcg acgcctttga acacgaagtg
     1561 gaagttcgcg cggccgaagt agtcttgata aagcggcgga tccgtgccga gctcgtggtt
     1621 gccgagcgtc gtgaagtacg gaacatcgag cgcgaggagc tctcgttggt actgctcggt
     1681 ttgctcgagc gtgcctcgct gagtcagatc gcccgcgccg agcaagaagc gcacaccggg
     1741 ctcgacgttc aggcgccgaa agatgtcgga tacgcggtcg atggcctctt gcacgtcgct
     1801 catcaaagcg aagcggaacg gcgcctcgga ctccgagtcg ttcggcgcga cgcgaaaggt
     1861 cgacacccca ggtggtagct cgagctcgaa gacgtggcgc gtcggcacgg gtggtgcgag
     1921 ctctcgcagc gtcaccggcg ccgtcgtcgc gattgagacg agctcggcgt cgaccagcgt
     1981 gttttgaagc tctagccgcg tggtcgtagc gcggtcgacc tcgagcgaga aagtgaacgt
     2041 cggcgcgctc ccccacaaaa cgatggcgtc ggcgctgatt tctcgtaccg ccgcgaggcc
     2101 ctggtctacc cgtacggcga cgttctgcgc ggtggcttgg ccaatcacga ggtcccgctc
     2161 cgcgcgctgc tcggagggtc gtgcgcaacc gagggcaagc aagagcggca ggcaagcgag
     2221 tgagagcagc cgagtcatgg cgatcgtacc cttcgttgga gctctggttg acgcagaagg
     2281 agcgatgcac cgaaaaacca cgcggagccc acgttgacgt ctgtgagcac gccgaagctc
     2341 gagttgaagt accacttcgt ctggaagcca aagtggccgg ccacgccgct gccgagcccc
     2401 gtgactttga ggcccgccgc gaagtcgtcg tggcgatggt cgtagtagag cagcgcctcg
     2461 ctccccggcg cgctcttgcc acgaagcctc acaccaaagc cgaagcggcc gagcagcaga
     2521 tcgtagctgt cggcaggtac gtgctggccc tcgatgtcgt agctgatggc gccgcgcgcg
     2581 tagccgagcg cgaactcggc gaaggagccg cggagcgagc gaccgacgtg agcgagatcg
     2641 taacgggcca gcaccgaggc ttcgccgctc gtcatcgcga agcgctcggt tggatacgtg
     2701 tgtcgcacga agcccaaatc gagctcgagc cggtcggcgt aacgtgaatc cgcgacgtcg
     2761 ctgtcgtcga ggaagcgata aagcacgtcc gcgcggtacc gagcgttgcc cgcccccatc
     2821 gagaagaacc ccaagagacc gagcccgaac gagccctgat tcaagcgcat tccttcgacg
     2881 agaaagtcgt gatgcgagaa ctgaacgtcc tcgaggcggc gatagccgaa ctccgtttcg
     2941 accttcggta acattgggcg ccgcgctgcg ccctgatccg atgcggcagt accgtacacg
     3001 tcagcgagca gcgatgtgcc gaatacacca aaccccatca tcgcgatcgc cgccgcgggc
     3061 ccaacgaccc agcgcgatgc gccactcgcg aagaggacgc cgcctccacc caaaatcata
     3121 ctgaggccga cgccttcgag tgcgagcagc tgccccgccg tgtcgcgctc cccgatgaca
     3181 tagtgaccgg cgccgtggac cagcgcgccc ggcaggaccg ctgccgttac gccgagtgct
     3241 cgtcggcccg gcatgacggg ctcgccgccc gactcggtgc gggtgctcgc cgtctccgct
     3301 cgcggttccg ctcgtggctc ggcgccgcgc gcgaggcggc acagcgaggt gagcgctaga
     3361 aaagcaacgg tggcggagca gcgcaagctc ggccagggta gcgcagtttt cgcagacgag
     3421 gcggtgaagc gcgaacgagc gcgggcgcgt gtgtcccggg cgttggtctg tcgtgaagcg
     3481 gggcgttttc aacgctctgt taagcgcccg ggcgtgtcgg taagttccgt gccgcaccgg
     3541 caacgctctt cagccggctg atgaactgaa gcgcctcctt gcggctcgga tacgtcccga
     3601 ggggcgccgg gagctctcgt cccttcacga tcactcgata catctgcttg gtgccagggc
     3661 gaggggcctg cttttcgacc tgaagcatcc tgagctcgtc gaacgagatc tcgtacgcgg
     3721 gtttgcggaa cagggcgcgg ctgcgcaccg tgcggcgctg cagatcgacg tggaggcggt
     3781 cgttgctggc gattatgccg agcaccgcgg ccccaaaaaa cggcagcaag aaagcgagga
     3841 gcacgatctt gccgacccag ctcagcaaga ggctgcacgc gatgagcacg aatacggctg
     3901 tcgcgcagag cgcccagccg aacgtgctcc aaaagcgcgc cgcgaacggc ttctcgtaaa
     3961 agagcagcgt ttcgccccga tcgacgacgc tttggtcgtg cccggaccag gcgttgatca
     4021 cccagtaagc agcggtaaaa gcggcgagca cgacgagcgc gcgcggcagc aagtaggcga
     4081 gccgcgcgtc gagggcgggg tttctgacgg tgccgtgggc gctgtagtac ggaatcgtaa
     4141 tccgactcgc ccacatcgct aggcccacgg cagtcatgtt cgcacgctgt gcccccgcgt
     4201 gccacctcga aagcgctgca agcgcctccg gggcttcagg ctcggctcgc tcgaaggcgc
     4261 agagccctgc tttctgagag ccgagcaaag gggttcagcg gagcacggcg cagtttagag
     4321 cgggccccag tgcgatgttg ttcgcgtcgt tgcactcggc gaccgtgttg agcggatcta
     4381 ccgaagcgta aagagtcacg ttgcggtaga tcgtctcgag ctccacgtgg aacctcacga
     4441 ggtcgcctgc cgcgagcggt cccgccagcg ttgtctcgcc gatcacctgg cctccgcggc
     4501 tcggatcccc agcgtagatt cgaacgagga tgttcgtcgc ttctgcggag ccgaggttcg
     4561 cgacggtcac cgttgcgtcg ggcggcgagc aaccaaccgc cttgtcgatg gcgacgatca
     4621 agtcggcgag ggcggcgccc gcctccaccg gagccgcgat ttgattgttg tcttcgtggc
     4681 actcgcgctc ggcttgcttg gcgtcgaccg tcgccttcac ttcgaccggc aaatccgggc
     4741 ggccgttgtt gccggcggtg taggtcacgc tgaccacggt cgaggcacct ggctcgaggc
     4801 tcgtgtccag cgtgacggtg atgggagcgc cgttggcatc cagtagctcc gcggtcacgg
     4861 ccggcgtcat ccaactgccg tcgaagccga tttgaacgcc cgggccgacg cgcaggtcac
     4921 ccgcgttctt cacgagcacc gaaatctcga tttggttcga gagctgcccg cacgcaacgt
     4981 cgggcgacgt tatctggatc gcgatgggga tcagatcggg agcgacaccg tagttgcgtg
     5041 gttgagagcg gtaggtgttg tagagcctgc cgttcagcgg cttgtagtgc tcgggctcgt
     5101 gcagcggaat ttgcccgccc tcggtcacgt tggtcacgtg atacgagtgc tcgttccaga
     5161 cgcggcgcgc agcgacccac acgtcgctcg gatcgcccca cacctcgagc ccgttcggta
     5221 ggctggtggc ggggacgtcg ttgttgccct tcggccagct atcgaggttc gcttcactgc
     5281 actggatcgt ctcgttattc tgcacgaaaa cgatctccgc gttgccgtcg ttgtcgacgt
     5341 cggcaacgac cggattttcc acgatggtgc ggctgagcga tggcagcgcg aggtagaggc
     5401 cgcccgtcgc tccgtcgtag acgtggaagt agcactcgtc gttgtaaacg acttcggccg
     5461 cgccatcgcc gttgaagtcg aacacgctcg acgacgtcac tttgctcgag tcgtcctcgg
     5521 tgtcgcgctt ccagccgctt tgcaggcaca cgcacgtgcc cgccttttgg ttacaaaccg
     5581 cgccggcgtt gcagtcggcg tccttcgtgc acgcgccgcc cggcgttcgc gccgggttc
//
LOCUS       DASTKS010000865         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1925587, whole genome shotgun sequence.
ACCESSION   DASTKS010000865 DASTKS010000000
VERSION     DASTKS010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925587"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /gene="dnaE"
                     /locus_tag="VFQ35_18020"
     CDS             <1..2235
                     /gene="dnaE"
                     /locus_tag="VFQ35_18020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9932608.1"
                     /translation="KDVARAMGIPAPEAQRMASLVPDKGQGKTYTIDEALEVEPKLKA
                     LSDSDPRVAELLKHSRKLEGLTRHAGMHAAGVVISEGPLADHVPCFKSDATIVTQYDK
                     DDVEAAGLVKFDFLGLKTLTVIDIAEKLVNARPDRANNPLSLSKIPLDDRETYQLITS
                     GETTGVFQLESSGMQQLLRQLKPDCFEDVVAVVALYRPGPLGTGMIDDFIGGKHGKKA
                     IRKLHPLVDDVLAPTYGVPVYQEQVMQIAQKLAGFTLGGADLLRRAMGKKKAEEMQKQ
                     QKLFVEGAAKNGVSEEMASSIFKEIEGFASYGFNKSHSAAYAVITYQTGYLKAHYPTE
                     FFAAALTADKDKIEKVVRTIAEARAWGVSVLPPDINASETDFTVVYSHPDGRGPLGGS
                     GKLRDRLGPRIRFGLGAVRGVGEAALETMFEARQSGGPFRDLFDFATRVDAKRLNKGV
                     LEALVQCGAFDSVLEPRGITRARAFGAIDRALERSRSASRDRERGQTTLFGMFDQARG
                     DGSSTAGDEYPVQSAEWDKMELLRREKAALGCYVSGHPLFRYQSKLGRLQVVASPKVA
                     DQEPWSMAIVAGMVENYQERLFKGGAGGRAAFFEIEDMFGRVKAKLRGDRIETYAHLL
                     TSSEAVIVSGKVSFPMTEEPDEEREPTLLVDQVELLADAALKATRSVTIRLDAQKTAR
                     SELTALKELLEGSPGPIPVELVLALGEGAEAVLDLPGARITPSDGLLGGLERVFGDPV
                     AELR"
     gene            2326..>2815
                     /locus_tag="VFQ35_18025"
     CDS             2326..>2815
                     /locus_tag="VFQ35_18025"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9932609.1"
                     /translation="MRDPRAELVRIDSHGQAHAIGSVASQRLRSREGIYRVLPSPEHV
                     VFMRFTGEDGRRDASDGAVVRLAGEIVAPGALCDIVALLGQTGWRGELVVLDSEHTRS
                     IFFDSGNIVGASTTAEPERIGQVLYKFGAIDATQRDRVLEAQAGGKRFGEVLLELGWL
                     SRE"
BASE COUNT          516 a          905 c          950 g          444 t
ORIGIN      
        1 aaggacgtcg cgcgcgccat gggcatccca gcgcccgaag cgcaacgcat ggcgagcctc
       61 gtccccgaca aagggcaggg caaaacctac acgatcgacg aggcgctcga agtcgagccc
      121 aagttgaagg cgctctcgga ctccgacccg cgcgtcgccg agctcctcaa gcacagccgc
      181 aagctcgaag ggctcacgcg ccacgctggt atgcacgccg ccggcgtcgt gatcagcgaa
      241 ggcccgctcg ccgaccacgt accgtgcttc aagagcgacg ccacgatcgt cacgcagtac
      301 gacaaagacg acgtcgaagc ggccgggctc gtgaagttcg acttcttggg tctgaagacg
      361 ctcaccgtca tcgacatcgc cgagaagctc gtgaacgcgc gcccggatcg cgcaaacaac
      421 ccgctcagcc tctcgaagat ccccctcgac gatcgcgaga cgtatcagct catcaccagc
      481 ggtgaaacga cgggcgtctt tcagctcgag tcgagcggca tgcagcagct cttgcgtcag
      541 ctgaagcccg actgcttcga agacgtggtc gccgtcgtgg ccctctaccg tcccggtccg
      601 ctcggcaccg gcatgatcga cgacttcatc ggcggcaagc acggcaagaa ggcgatccgc
      661 aagctgcacc cgctggtcga cgacgtgctc gcgccgacgt acggcgtgcc cgtttaccaa
      721 gagcaggtga tgcagatcgc ccagaagctc gccggcttta cgctgggcgg cgccgacctt
      781 cttcgccgcg cgatgggcaa gaagaaggcg gaggagatgc agaagcagca aaagctcttc
      841 gtggagggcg ctgcgaagaa cggcgtctcc gaggagatgg cctcgagcat cttcaaagag
      901 atcgagggct tcgcgtctta cggcttcaac aagagtcact ccgccgccta cgcggtcatc
      961 acttatcaaa ccggttatct aaaggcgcat tacccgaccg agttcttcgc ggcggcgctc
     1021 acggcggaca aggacaagat cgagaaggtg gtccgcacca tcgcggaggc gcgcgcctgg
     1081 ggagtcagcg tattgccccc cgacatcaac gcgagcgaaa ccgacttcac cgtcgtctac
     1141 tcgcacccgg acgggcgggg tcccctcggc ggcagcggca agctcagaga tcgattgggg
     1201 ccgcgcattc gtttcggtct cggcgccgtc cgcggcgtcg gcgaagcggc cctcgagacg
     1261 atgttcgaag cgcgacagag cggcggcccc ttccgcgact tgttcgactt tgcgacgcgc
     1321 gtcgacgcga agcgtttgaa caaaggtgtc ctcgaagcgt tggtgcagtg cggcgctttc
     1381 gactcggtgc tcgaacctcg aggcatcact cgcgctcggg cgttcggcgc catcgaccgg
     1441 gcgctcgagc ggtcccggag cgcgagccgc gatcgcgagc gcgggcaaac gacgctgttc
     1501 ggcatgttcg accaagcgcg cggcgatggc tcctcgactg ccggtgacga gtacccggtg
     1561 caatcggcgg agtgggacaa gatggagcta ttgcgccgcg agaaggccgc gctcggctgt
     1621 tacgtttcgg gtcacccgct gtttcgctat cagagcaagc tcggccgcct gcaagtcgtc
     1681 gcgagcccga aggtcgccga tcaggaaccg tggtcgatgg cgatcgtggc cggcatggtc
     1741 gaaaactatc aagagcgcct attcaagggc ggcgccggag gacgcgcggc gttcttcgaa
     1801 atcgaggaca tgttcgggcg cgtcaaagcg aagctgcgcg gcgaccgcat cgagacgtac
     1861 gcccaccttt tgacctcgag cgaagccgta atcgtgtcgg ggaaggtgag tttcccgatg
     1921 accgaggaac ccgacgaaga gagagagccg acgctgctcg tcgatcaggt cgagctcttg
     1981 gcagacgccg ccctgaaggc gacgcgcagc gtgaccattc gcctggacgc gcagaagacg
     2041 gcgcgttcgg agctgaccgc gttgaaggag ctactcgaag gctcgcccgg gcccatcccg
     2101 gtcgagttgg tgctcgcgct cggtgagggg gccgaggcag tcctagacct tccgggagcg
     2161 cgcattaccc cctcggacgg cttgctgggc ggcctcgagc gcgtgttcgg cgatccggtg
     2221 gcggaattac gctagcccgc tccaccgtca aacgcgtgac ccgcgcggct ggcccggatc
     2281 ttggcggact ccgagagagc ccgtagactg agggctggtt cagaaatgcg agatccgcgc
     2341 gcagagctcg tccgtatcga ctcgcacggg caggctcacg cgatcgggag cgtggcgagt
     2401 cagcgcctgc ggtcgcgcga aggcatttac cgcgtgcttc cctctccgga gcacgtcgtg
     2461 ttcatgcgct tcaccggcga agacgggcgc cgcgacgcga gtgacggcgc ggtggtgcga
     2521 ctcgcgggcg agatcgtggc ccccggcgcg ctctgcgata tcgtcgcgtt gctggggcag
     2581 acgggctggc gcggggagct cgtcgtgctc gacagcgagc acacgcgaag catcttcttc
     2641 gacagcggca acatcgtcgg cgcctcgacg acggccgagc cggaacgcat tggccaggtg
     2701 ctgtacaagt tcggagcgat cgacgcgacc cagcgagatc gcgtgctgga agcgcaggcc
     2761 ggcggtaagc gcttcggcga ggtcttgctc gagcttggct ggctctctcg cgagc
//
LOCUS       DASTKS010000866         7008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1030818, whole genome shotgun sequence.
ACCESSION   DASTKS010000866 DASTKS010000000
VERSION     DASTKS010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030818"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFQ35_18030"
     CDS             complement(<1..541)
                     /locus_tag="VFQ35_18030"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9932610.1"
                     /translation="MTLQFSRPNADVFVPDASSAESALGRTTHLAVAAHQDDIEIMAA
                     HGILSCFGKTDAFFTGVVVTDGAGSARSGIYAHFDDGAMAAVRRREQRKAAVVGEYSA
                     QVQLGFSSANVKARDGTERDALLADLVKLFTLARPRIVYTHNIADKHDTHVAVTLRVL
                     DALRSVPRDARPERVIGCEV"
     gene            complement(538..1938)
                     /locus_tag="VFQ35_18035"
     CDS             complement(538..1938)
                     /locus_tag="VFQ35_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9932611.1"
                     /translation="MTLETNPRITPPLDPDFRPIALARRRFRGMVSDAKSSVPLVFAL
                     ERDAGKVSVFRCSVLPDSHARAALNAAFAERLLKTLLWQRGGGRVLVAGPDSAVADLP
                     AMYAEGGARAFDAKFFSEVYGREFSVERVASDRVPEPRESARRLGGDFRGHRIGFDAG
                     GSDCKVAALAEGQLVFAEEVPWLPKEHADSEYHVRIVRDCIEKARSKLPKLDAIGVSS
                     AGIFVDNRTRVASLFRRVPQPEFDAHIRNIYADLGDLWGVPVEVANDGDVAALAGALE
                     LERGALLGLAFGTSLAAGYVDASRRVQGYLNELAFAPHDVAESAPLDSEWSGDRGTSV
                     NYLSQDAALRLAPRAGIDLTEGTRAEQLEALQRMVSDGDPRAAAVFRSLGVYLGYALI
                     DYSEFYDMQDVLLLGRVTSGSGGSELVQAARSVLALEAPELASRLGLHLPDESRRRVG
                     QAIAAASLPVLSGDAS"
     gene            1992..2288
                     /locus_tag="VFQ35_18040"
     CDS             1992..2288
                     /locus_tag="VFQ35_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932612.1"
                     /translation="MRPLGVTNVALNGLNRQIAAFERSASEVVEKTTSEDGFEPVDIS
                     HEAAKAGESLDSATHGSLEGALVDLRISKYLAIANMKVLKTADEMQRTAQEIVR"
     gene            2480..4042
                     /locus_tag="VFQ35_18045"
     CDS             2480..4042
                     /locus_tag="VFQ35_18045"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET9932613.1"
                     /translation="MPRYLGLDSSTQSLSALVIDTDTGEVVLDEALNFGKELPEFGSP
                     NGFLPNPDPQIKHSNPLMWVEALDRLLSRIKAQGFDLGSIQGISGAGQQHGSVYLNTP
                     ITEAAQWTERASLAEQVRPLLSRASSPIWMDSSTNAECREIAEAVGGDARVVEITGSR
                     AIERFTGPQIRKFYKTEPAAYEATREIHLVSSFIASLLVGTSAPIDFGDGAGMNLLDL
                     AALTWSKPLLEATAPRLEGRLRPPVASNTRVGSIAPYFVHRFGFAAGTGVVAFTGDNP
                     SSLVGMGATRPGTAVVSLGTSDTVFASMSAPRTDPRGFGHVFGNPGGGFMCLICFANG
                     SLAREKVKDRVGLDWPGFEKAIVDQTKPGNDGALLLPYFVPEMTPKLLKPEPHLYGDE
                     AFRDYREPARAARAIVEAQALAMQRYSRFIGEAPTRILATGGASRNAGILQVIADVFQ
                     AEVRTLHVGNSSALGGALRAANALGGDSFDSLFERFAAPDPKLCFRPNANADYAALRT
                     RFETEVDRLTSA"
     gene            4202..4951
                     /locus_tag="VFQ35_18050"
     CDS             4202..4951
                     /locus_tag="VFQ35_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932614.1"
                     /translation="MSRGLGARAGFAFGVSALVCGSGCATSEDIPLDQLERGGRSAEL
                     GGAPGSGGAESGGTTSGLGGTTAADGGTTLGAGGKASGSGGAMGGAGRTGSGSGGRTA
                     TGSGGRTGTGLGSGGRTAAGSGGRTGFGTGGRTGTAGQSSTAGAGSCDWTGCATSNCT
                     AGCPTTDGGYCSKACTDMVTCFQMNPGCSKSGDPLCVMRNNGAANVCTTKWESAGGQQ
                     TGPAATGPSQAAARYISCACDLTIPGVTPGM"
     gene            complement(4977..>7008)
                     /gene="glgB"
                     /locus_tag="VFQ35_18055"
     CDS             complement(4977..>7008)
                     /gene="glgB"
                     /locus_tag="VFQ35_18055"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET9932615.1"
                     /translation="AFSESRPMTALGDGLFAAFLPGVKLPLEYRLRFQFADGADWERD
                     DPYRFLPTLGDIDQHLIREGTHRRLWEALGARARTAEGTSGTAFAVWAPSARRVSVVG
                     SFNRWDGRLFPMRALGSSGIWEIFVPGVGPGELYKFEIKTQDGALRLKTDPMAREMEL
                     PPATASRVNVSNYSWGDEAWIAARAGRDVRREPVSIYELHLGSWARVPEEGNRWLSYR
                     EIAPRLVAHVQRLGFTHVELMPIAEHAFYPSWGYQVTGYFAPTSRYGTPDDFRYFVDY
                     CHQHGVGVIIDWVPAHFPKDDFSLRRFDGTALYEHEDSRRGEHPDWGTLIFNYGRREV
                     QNFLVANALYWLKEFHVDGLRVDAVASMLYLDYSRKEGEWVPNPFGGRENLEAIEFLR
                     AVNSVIHQEVPGAFTVAEESTAWGGITRTPVEGGLGFTFKWNMGWMHDTLAFFSKEPV
                     HRKFHVDQLTFAMLYENTENFINSISHDEVVHGKGALVEKMPGDFWQKLANLRLLLVY
                     QYTRPGKQLVFMGTEFAQHHEWNCDNSLDWHIAEHPQRRGLQDFLAELGRLYRATPAL
                     WRADPNPESFEWIDCTDKDNTVLSYLRREGNDYVIVVLNFTPVPREDYRIGVPAPGRY
                     VERLSSDDLRFGGSEFETLTVVEADPVPAHNRQYSLKLRIPPLGALVLTASR"
BASE COUNT         1259 a         2320 c         2311 g         1118 t
ORIGIN      
        1 agacctcaca gccgatgacg cgctcggggc gcgcgtcgcg cggaaccgac cgaagcgcgt
       61 cgagcacccg taacgtcacc gcgacgtgcg tgtcgtgctt gtcggcgatg ttgtgcgtgt
      121 acacgattcg agggcgagcg agcgtgaaga gcttcaccaa gtcggcgagc agcgcgtcgc
      181 gctcggtgcc atcgcgcgcc ttgacgttcg cgctcgagaa gccgagctga acctgagcag
      241 agtattcccc caccaccgct gctttgcgct gctcccggcg acgcaccgcc gccatcgcgc
      301 cgtcgtcgaa gtgggcataa atcccgctgc gagcgctgcc ggcaccgtcg gtgacgacca
      361 cccccgtgaa gaaggcgtcc gtctttccga aacaggacaa gatcccatgc gcagccatga
      421 tctcgatgtc gtcttgatga gcagccacgg cgagatgggt cgtgcgcccg agcgcgctct
      481 ccgcactgct cgcgtcgggg acgaacacgt cggcgttggg ccgcgaaaat tggagcgtca
      541 cgacgcatct cccgacagca ccggcagact cgcggcggcg atggcctgac cgacgcgacg
      601 acgcgactcg tccggcaggt gcaagccgag gcgcgaagcg agctccggcg cctcgagcgc
      661 gagcaccgaa cgcgcagctt gcacgagctc acttccgcca gatccagagg tcacccgccc
      721 gagcagaagc acgtcttgca tgtcgtagaa ctccgaataa tcgatgagcg catagccgag
      781 ataaacgccg agcgagcgaa aaaccgccgc cgctcgaggg tcgccatcgc tcaccatgcg
      841 ctggagcgct tcgagctgct cggcgcgcgt gccctcggtg aggtcgatgc ccgcgcgcgg
      901 cgcgaggcgc aacgccgcgt cttgcgacag gtagttgacc gatgttccgc gatctcccga
      961 ccattcgctg tcgagcggcg ccgactcggc gacgtcgtgc ggagcaaacg cgagctcgtt
     1021 gaggtagccc tgcacccggc gcgaggcgtc gacgtagccc gccgcgaggc tcgttccaaa
     1081 ggcgaggccg agcagcgcgc cacgctcgag ctccagcgct cccgcgagcg cggcgacgtc
     1141 accatcgttc gcgacctcga ccggtactcc ccagaggtcg ccgaggtcgg cgtaaatatt
     1201 gcgtatgtgc gcgtcgaatt ctggctgcgg gacgcgacgg aagagcgacg cgacgcgggt
     1261 gcggttgtcc acgaagatgc cggcagagct cacgccgatc gcgtcgagct tcggcaactt
     1321 ggagcgagcc ttctcgatgc agtcgcgcac gatgcgcacg tggtactcgg agtcggcgtg
     1381 ctccttcggg agccagggca cttcctccgc gaacaccagc tggccttcgg cgagagctgc
     1441 cactttgcag tcgctgcccc cggcgtcgaa gccgatgcga tgaccgcgaa agtcgccgcc
     1501 caagcgtcgc gcgctctcgc gcggctcggg cacgcggtcc gatgcgacgc gctccaccga
     1561 aaactcccgc ccgtagacct cgctgaagaa cttggcgtcg aatgcgcgcg cgccgccctc
     1621 cgcatacatc gcgggaaggt ctgcaaccgc gctgtcaggc ccggccacga ggacccggcc
     1681 gcccccgcgt tgccaaagca acgtcttcag caagcgctct gcaaaagctg cgttcagtgc
     1741 cgcgcgcgcg tgcgagtcgg gaaggaccga acagcgaaac accgacacct ttcccgcatc
     1801 gcgctcgagc gcgaacacga gcggaacaga gctcttggcg tcgctcacca tccctcgaaa
     1861 gcgacgccga gcgagtgcaa ttgggcgaaa atccggatcc agcggaggtg tgatccgagg
     1921 gtttgtctcg agcgtcataa ttttgacaat tcaactcaag gccactgccg aaaaaccgtt
     1981 cacccggttg aatgcggccc ctcggcgtaa ccaatgtcgc cctcaacggc ttgaaccggc
     2041 aaattgccgc gttcgaacgt tcggcatcag aggtcgtcga gaaaaccaca tccgaggacg
     2101 gattcgaacc ggtagacatc tcccacgaag cggcgaaagc cggggagagc ctcgactcgg
     2161 ccacgcacgg ctcgctcgaa ggagccttgg tcgaccttcg catctccaag tacctcgcga
     2221 tcgcgaacat gaaagtcctc aaaaccgcgg acgaaatgca gcgcaccgcg caagaaatag
     2281 ttcgctagaa gcggcagcca aatcacgttt tccggtcaga tctgcccctc accccttaac
     2341 tctggtagca gagaggccgt ttgagggggg catacgcggg ggaaggggac ctgcctcgca
     2401 cctgtcgacc cggcggccga tgtacgccgt acgagcgcga tccgcctgac cgccgaagcc
     2461 gagtatgctc gcggccccca tgcctcgtta cctcggcctc gattccagca ctcaaagcct
     2521 gtccgccctc gtcatcgaca ccgacaccgg agaggtcgtt ctcgacgaag cgctgaactt
     2581 cggaaaagag cttcccgaat tcggatctcc gaacggcttc cttccgaacc cggatccgca
     2641 gatcaagcat tcgaacccgc tgatgtgggt cgaggcgctc gatcggttgt tgtcgcgcat
     2701 caaggcgcag ggcttcgatc tcgggagcat ccaaggcatc agcggagccg gacagcagca
     2761 cggttcggtg tacctgaaca cgccgatcac cgaggccgcg caatggaccg agcgagcgag
     2821 cttggcggag caagtgcgac cgctcttgag ccgcgcgtcg tcgcccatct ggatggacag
     2881 ctcgaccaac gcagagtgcc gcgagatcgc ggaggcggtc ggcggcgacg cgcgcgtcgt
     2941 cgaaatcact ggttcgcgcg ccatcgagcg cttcacgggg ccgcaaatcc gcaagttcta
     3001 caagaccgag cccgccgcgt acgaagcgac tcgtgagatt cacctggtga gcagcttcat
     3061 cgcgtccctg ctcgtgggca cctcggcgcc gatcgacttc ggcgatggcg ctggaatgaa
     3121 cttgctcgat ctcgccgcgc tcacgtggag caagccgctg ctcgaggcga ccgcgccgcg
     3181 cctcgaaggc cgcttgcgcc ctccggtcgc gtcgaacacg cgcgtcggca gcatcgcgcc
     3241 gtacttcgtg caccgcttcg gttttgcggc gggcaccggc gtcgtcgctt tcacgggaga
     3301 caacccgagc agcttggtgg gcatgggcgc aacgcgacct ggtaccgcgg tcgtcagcct
     3361 aggaacgagc gacaccgtgt tcgcttcgat gagcgcgccg cgcacggacc cgcgcggttt
     3421 cggtcacgtc ttcgggaacc ctggcggtgg tttcatgtgc ctcatctgct tcgccaacgg
     3481 ctcgctcgcg cgcgaaaaag tgaaagaccg cgtcggcctc gattggccag ggttcgaaaa
     3541 ggctatcgtc gatcaaacga agccgggcaa cgacggcgcg ctcttgctgc cgtacttcgt
     3601 gcccgagatg acgccgaagc tgttgaagcc ggagccgcac ctctacggcg acgaagcatt
     3661 ccgcgactat cgagaaccgg ctcgcgcggc gcgcgcaatc gtcgaggcgc aggcgctcgc
     3721 gatgcagcgc tactcgcggt tcatcggcga agccccgacg cggatcctcg cgaccggcgg
     3781 cgcgtcgcgc aacgccggca ttctccaggt cattgccgac gtcttccaag cggaagttcg
     3841 aacgctgcac gtcggcaatt cttcggcgct cggcggcgct ttgcgcgcgg cgaacgcgct
     3901 cggcggcgac agcttcgact cgttgttcga gcgcttcgcg gcgccggatc cgaagctctg
     3961 cttccgaccg aacgcgaacg ccgactacgc cgcgctgcgc acacgcttcg agaccgaagt
     4021 cgatcggcta acgagcgcat gacgcgttgc tcgcacgtgg gtccgtatcc gacctggtcg
     4081 tcgcgaggcc tcactcggca cagtccctcc agaattttcc ggaacgagcc ccgcgaccgc
     4141 ggcggcccct gacggcggcc tcttgcgcgg ctccggccgc tgagatacgg ccgaagactg
     4201 gatgtcgaga ggccttggtg cgcgcgctgg gtttgccttc ggggtgagcg ccctggtgtg
     4261 cggcagcggc tgcgcgacga gtgaggacat cccgctcgat cagctcgagc gcggcggtcg
     4321 cagcgcggag ctcggtggcg cgccgggttc gggcggagcc gagagtggtg gaacgacttc
     4381 cggattgggc ggcacgaccg ccgccgatgg cggtacgacg ctcggggccg gcggcaaagc
     4441 gagcggctcc ggcggcgcga tgggtggcgc cggcagaact ggttcaggta gcggcggcag
     4501 aactgcgacc ggcagcggtg gcaggaccgg caccgggctc ggtagcggcg gtagaaccgc
     4561 ggccggcagc ggcggcagaa ccgggttcgg taccggtggc agaactggaa cggcgggaca
     4621 gtcctccacg gctggcgctg gttcctgcga ttggacaggg tgcgccacct cgaactgcac
     4681 cgctggttgc ccgacgacgg acggcggtta ctgctccaaa gcgtgcaccg acatggtgac
     4741 gtgcttccaa atgaatcctg gctgcagcaa atccggggat ccgctgtgcg tcatgcgcaa
     4801 caacggcgcc gccaacgtgt gcacgaccaa gtgggagtcc gcgggcgggc agcagactgg
     4861 tcccgccgcg accggccctt ctcaagccgc agcgcgctac atctcttgcg cgtgcgatct
     4921 cacgatcccc ggcgtaacgc cagggatgtg agcgccgggc tcgaggcgaa gtccgctcaa
     4981 cgcgaggccg tgagaacgag cgcgcctagc ggcgggatgc gcagcttcag cgagtattga
     5041 cgattgtgcg ccggcaccgg gtccgcttcg accaccgtca gcgtctcgaa ctcactaccg
     5101 ccgaagcgga gatcgtcgct cgagagtcgc tcgacgtaac gtccgggagc tggaacgccg
     5161 atgcgatagt cctcacgcgg caccggagtg aagttcagga cgacgatgac gtagtcgttg
     5221 ccctcacggc gcaggtagct gagcaccgtg ttgtccttgt cggtgcagtc gatccactcg
     5281 aagctctctg gattcgggtc cgctcgccaa agcgccgggg tcgcgcggta caggcgaccg
     5341 agctccgcga gaaaatcttg caggccgcgg cgctgcgggt gctcggcgat gtgccaatcg
     5401 agcgagttgt cgcagttcca ctcgtggtgc tgcgcgaact cggtgcccat gaacacgagc
     5461 tgcttaccgg gacgggtgta ctggtaaacc agcaagagcc ggaggtttgc gagcttctgc
     5521 cagaagtcgc ccggcatctt ctcgacgagg gcgcctttgc cgtgaaccac ctcgtcgtga
     5581 gaaatcgagt tgatgaagtt ctcggtgttc tcgtagagca tcgcgaacgt cagctggtcg
     5641 acgtggaact tccggtgcac cggctccttc gagaagaagg cgagcgtgtc gtgcatccag
     5701 cccatgttcc acttgaaggt gaagccgagg ccgccttcga ccggcgtgcg cgtgatgccg
     5761 ccccacgcgg tggactcctc cgcgacggtg aacgcgcccg gcacttcttg gtgaatcacg
     5821 ctgttcaccg ctcgcaagaa ctcgatcgcc tcgaggttct cacggccgcc gaacgggttc
     5881 ggtacccact cgccttcttt gcggctgtag tcgaggtaca gcatggacgc gaccgcgtcg
     5941 acacgcagac cgtcgacgtg gaactctttc agccagtaga gcgcgttcgc caccaagaag
     6001 ttctgaacct cgcggcggcc gtagttgaag atgagcgttc cccaatccgg atgctcaccg
     6061 cggcgcgagt cttcgtgctc gtagagggcg gtgccgtcga agcggcgcag cgagaagtcg
     6121 tccttcggga aatgcgccgg aacccagtcg atgatgacgc cgaccccgtg ctggtgacag
     6181 tagtcgacga aatagcgaaa gtcgtccggc gtgccgtagc gcgacgtcgg cgcgaagtag
     6241 ccggtcacct ggtaacccca ggaggggtag aaggcgtgct cggcgatcgg catcagctcg
     6301 acgtgcgtga agccgaggcg ctgcacgtgc gccacgaggc gcggcgcgat ttcacggtac
     6361 gagagccagc ggtttccctc ttcgggaacg cgcgcccacg agccgagatg aagctcgtag
     6421 atcgagactg gctcgcgccg aacgtctcgc ccggctcgcg ccgcgatcca cgcttcgtca
     6481 ccccagctgt agttcgatac gttcacgcgc gaagcggtcg cgggcggcaa ctccatctcg
     6541 cgcgccatcg gatccgtctt caggcggagg gcgccgtctt gggttttgat ctcgaacttg
     6601 tagagctcgc ccgggccgac tccgggaacg aagatctccc aaatgccgga gctacccagc
     6661 gcgcgcatcg ggaaaagccg gccgtcccag cggttgaagg agccgaccac gctgacgcgg
     6721 cgagcgctcg gcgcccacac cgcgaacgca gtcccgctgg tgccttcggc ggtgcgcgcg
     6781 cgggccccga gggcctccca cagcctccgg tgcgttccct ctcggatgag gtgctggtcg
     6841 atgtcaccga gggtcggcaa gaagcgatac gggtcgtcgc gctcccaatc ggcgccgtcg
     6901 gcgaactgaa accgcagccg atactcgagc ggcagcttca cgccggggag gaacgcggcg
     6961 aacaaaccgt caccgagcgc cgtcatgggc cgcgattccg aaaaagcg
//
LOCUS       DASTKS010000867         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1701577, whole genome shotgun sequence.
ACCESSION   DASTKS010000867 DASTKS010000000
VERSION     DASTKS010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701577"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VFQ35_18060"
     CDS             <1..1277
                     /locus_tag="VFQ35_18060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9932616.1"
                     /translation="WVDERLDPERSTEAACRYLGDLYRRFGSWELAMAAYNMGHGGLS
                     RSIRKYNTNDFWELSRYEASLPWETTLYVPKILATAIVMANKRTFGLADVEVDSPERY
                     DTVLVAGGVPLEDIARLSGVPSSSIEALNPQYLAGRTPPVAAQAAARSWPVRVPVGKG
                     DKVREALAREGPLDERFVPYVVRTGDTVESIAFARGATEAQLRALNSLDPRESLVPGT
                     VLLVPVSDTSRDNVAADNVVVVPPRQFEYPGRRRVFYRVVSGDTLGRVAEAFGTTQGD
                     LLLWNALDDGARLHVGLLLQMFVAVDRDLSKVRYLAESKAKILVAGSPEFFDYFEGQN
                     GKRRFVLRAKASDTLAAVGKRYGATTASMERINRRSRTDPIAAGEPIVVYTERARPVP
                     GDDLYVDARGRATQVRADSASGVAAVVDSAED"
     gene            1301..2701
                     /gene="glmU"
                     /locus_tag="VFQ35_18065"
     CDS             1301..2701
                     /gene="glmU"
                     /locus_tag="VFQ35_18065"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET9932617.1"
                     /translation="MASLTALVLAAGQGTRMKSRLPKVLHTVAGRPLLYYPIKAAFDA
                     GAEHAVIVSSGRSEIASELSRYFPADRISIAIQEVARGTGDAARIGLEKVNTDRVLIV
                     YGDTPLVRSSDLSSLVQALDAADRPPVSLLTCELENPQGYGRILRDAQGRVLGVREQK
                     DLANDAERAVREVNAGMYAADTRALRDAVSRIVPNNAQGEYYLTDVVEIVGGGRCVTV
                     RGHHEALVGVNDRSQLHSAELAMFQRIRERHARAGVTLRGDVYIDEGVELGEDAVLET
                     GVRLRGATRIGRGAFIDVGCVLDDVLVGDEVVVKPYSVLTKSTVGKAAQIGPFSHLRP
                     ESDIGDEAHIGNFVETKKTRVQKGAKANHLAYLGDGDVGPNANIGAGTIFCNYDGFQK
                     HRTVIGEGAFIGSDSQLVAPVTIGKGAYVGTGTTVTKDVPDEALAIGRARQENKDGYA
                     TRLRGRLKAARDAKKK"
     gene            complement(2825..>3125)
                     /locus_tag="VFQ35_18070"
     CDS             complement(2825..>3125)
                     /locus_tag="VFQ35_18070"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932618.1"
                     /translation="DARTYDVAAAILKEIGVGSIELLTNNPEKVESLARLGVEVERRV
                     PVLVAANPFSAGYLEVKRRRMQHDLPIVASTEAKNERTAAESEAPKSRNGRAAGY"
BASE COUNT          532 a         1013 c         1051 g          529 t
ORIGIN      
        1 gctgggtgga cgagcggctc gatccggagc gctcgacgga ggcggcgtgc cgctacctgg
       61 gcgacttgta taggcgcttc ggtagctggg agctcgcgat ggccgcgtac aacatggggc
      121 acggcgggct gtcccgctcg attcgtaagt acaacacgaa cgacttctgg gagctttcgc
      181 gttacgaggc ctcgctgccg tgggagacca cgctctacgt ccccaagatt ttggcgaccg
      241 ccatcgtgat ggcgaacaag cgcacgttcg gcctcgccga cgtcgaggta gattctcccg
      301 agcgctacga caccgtgctc gtcgctggtg gtgttccgct cgaagacatt gcgcgcttga
      361 gcggggtccc gagctcgagc atcgaagcct tgaatccgca gtacctcgcc gggcgtaccc
      421 cgcctgtcgc ggcgcaggcg gcggcgcgga gctggccggt gcgcgtgccg gtcgggaagg
      481 gtgacaaggt ccgcgaagcg ctcgcgcgcg aaggcccact cgacgagcgc ttcgtccctt
      541 acgtcgtccg aaccggtgac acggtggagt ccatcgcgtt tgcgcggggc gcgaccgaag
      601 cgcagcttcg cgctctaaac tcgctcgatc cgcgcgagag cctcgttccg gggacggtgc
      661 tgctcgtacc ggtctcggac acgtcgcgcg acaacgtggc cgccgacaac gtggtcgtgg
      721 tgccgccgcg gcagttcgag taccccgggc ggcgtcgagt cttctatcga gtcgtatcgg
      781 gcgacacgct gggacgagtc gcggaagcgt tcggaacgac tcagggcgac ctgctcttgt
      841 ggaacgcgct cgacgacggg gcgcgcctgc acgtcggcct gctcttgcag atgttcgtgg
      901 cagtggatcg cgatttgtcg aaggtacgct atctcgccga gtcgaaggcg aagatcctgg
      961 ttgcgggatc cccggagttc ttcgactact tcgaggggca aaatggcaag cgccgtttcg
     1021 tcctgcgcgc caaagcgagc gacaccctcg ccgcggttgg gaagcgatac ggggccacca
     1081 ccgcctccat ggagcggatc aaccgccgtt cccggacgga cccgatcgcg gcgggtgagc
     1141 ccatcgtcgt ttacaccgag cgcgctcgac cggttccggg cgacgacctt tacgtagacg
     1201 ctcgcggacg cgcgacccaa gttcgtgcgg actccgcctc tggagttgcg gccgtcgtcg
     1261 attccgccga agattagcgc ccctcaaaag gagaacgaac gtggcctcgc tcaccgcact
     1321 cgtactggct gccggtcagg gaactcgcat gaagagccgc ctgcccaagg tgctccacac
     1381 cgtggctggt cgacctctgc tctattaccc catcaaggca gctttcgacg ccggcgcaga
     1441 acacgccgtg atcgtgagca gcggcagaag cgagatcgcg agcgagcttt cgcgctactt
     1501 cccggccgat cgcatttcga tcgcgatcca agaggtggcg cgtggaaccg gcgatgccgc
     1561 gcgcattggt ttggagaagg tgaacaccga tcgcgtgctc atcgtctacg gcgacacgcc
     1621 gttggttcgc tcttcggatt tgtcgtcgct cgtgcaggcg ctcgacgccg ccgatcggcc
     1681 gcccgtctcg ctcctcactt gcgagctcga gaacccccag ggctacgggc gcatcttgcg
     1741 cgacgcgcag ggccgcgtgc tcggcgtgcg cgaacagaag gatttggcta acgacgccga
     1801 gcgcgccgtg cgcgaagtga acgcaggcat gtacgccgcc gacacgcgcg cgctgcgtga
     1861 cgccgtgtcg cgcatcgtgc ccaacaacgc gcagggtgaa tactacctga ccgacgtcgt
     1921 cgaaatcgtg ggcggcgggc gctgcgtgac ggtgcgtggg catcacgagg cgctcgtcgg
     1981 cgtcaatgat cgctcgcagc tgcacagcgc cgagcttgcg atgtttcaac gcattcgcga
     2041 gcgtcacgcg cgcgcggggg tcactctccg cggcgacgtt tacatcgacg agggcgtgga
     2101 gctcggcgaa gacgccgtgc tcgagacggg cgtgcgtcta cggggcgcga cgcgcatcgg
     2161 tcgcggcgct ttcatagacg tgggctgtgt gctcgacgac gtgctcgtcg gcgacgaggt
     2221 cgtcgtcaag ccgtactcgg tactcaccaa gagcacggtc ggcaaagccg cccagattgg
     2281 cccgttttcg cacctgcgtc ccgagtcgga catcggtgac gaagctcaca tcggcaactt
     2341 cgtcgagacc aaaaagaccc gcgtgcaaaa gggcgccaag gcgaaccacc tcgcgtacct
     2401 cggcgatggc gacgtgggtc ccaacgcgaa catcggcgcc ggcaccatct tctgcaacta
     2461 cgacggcttc caaaagcacc gcaccgtgat cggcgagggc gcgttcatcg gcagcgattc
     2521 tcagctggtc gcgccggtga cgatcggcaa aggggcttac gtcggcaccg gcactaccgt
     2581 caccaaagac gtccccgacg aagcgctcgc gattggccgc gcgcggcaag agaacaaaga
     2641 cggttacgcg acgcgtttgc gcggtcggct caaggccgcg cgcgacgcga agaagaagtg
     2701 acggagcagc gctgaagcga tgcgcccgct gcagagcggc gcatcgctcg cgaccccgaa
     2761 aataaggcac cgtctacgcg cccctcacac gaagcgtctc atcgctcggg ccgagctcgt
     2821 cgcgttagta gcccgcggcg cggccgttgc gtgacttcgg cgcttcgctc tcggcggcgg
     2881 tgcgctcgtt ttttgcttcg gtgctggcga cgatgggcag atcgtgctgc atgcgacggc
     2941 gctttacctc gaggtagcca gccgagaacg gattggcagc gaccagaacc ggcacgcgcc
     3001 gttcgacttc gacgccgagc cgggcgagcg actcgacctt ctccggattg ttggtgagaa
     3061 gctcgatcga gccgacgccg atctctttca aaatcgcggc cgcgacatcg taggtgcggg
     3121 catct
//
LOCUS       DASTKS010000868         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_139629, whole genome shotgun sequence.
ACCESSION   DASTKS010000868 DASTKS010000000
VERSION     DASTKS010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139629"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1543
                     /locus_tag="VFQ35_18075"
     CDS             137..1543
                     /locus_tag="VFQ35_18075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932619.1"
                     /translation="MDEVPERDVSLSGALIDGRYRVEYCIGSGGMGVVWRVRHVRSRQ
                     AFALKTLRTRATRDRKALRRILQEARAAAAIRSRHVVRVVDVKPDYVHEGTALPFIVM
                     ELLEGTSLADYLASVDRLSEGQVVWVMRAVSRGLSRAHEQGIIHRDLKPSNIFLARDD
                     DDGVVVKLCDFGIAKLHASAHSEASEPLTLSTDTGVILGTPRYMAPEQLRQSGSERPA
                     TDQWAFALVAFRALTGRGYFEQARSMAELLLAIVHDALPKPSRVSHLGAEFDAWFERS
                     CARDPEQRFASVEAQQRALEQALGTPEATPIAPADLQGSEAANEPGTRSASPSKEEPP
                     RAHLRPFYVALVAATLLSLVSTIRVAAELRPRPPPLREIAQVSTVSSAPARGRAAMTP
                     IESTSEIAKPAPAVALDDTAKAESTKPVSPEPPPKPLRRRGKPRAVSAMSPLLPLGAP
                     CARSSECRDGLCAAEVCQ"
     gene            1540..>2375
                     /locus_tag="VFQ35_18080"
     CDS             1540..>2375
                     /locus_tag="VFQ35_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932620.1"
                     /translation="MRRGLVGFALAAASSLFCFQASGDDARAAMTRAIAAKERAIDTA
                     ERSDWIRALELMQAADRLSSTAVSKYELGFVAAQLGEIALAVECYEAALSLDLQGSAA
                     EKARAFLERQRQASAESTATEQPRAVTPSSVPPPDPVSRTDPATQPRAERPKRHDAPL
                     LAPLQRPTEPASHTAKWVLLGSGATLSILALGFVPITERQLARRREVLRSACALQTDG
                     PDTCAHAKPGQWETAQSASDSIALWKTARNLSWVGLGVGVSVLAADGLALLLSAPTAR
                     SGA"
BASE COUNT          392 a          804 c          778 g          401 t
ORIGIN      
        1 cggtgcgtcg gcgggcgtgt tctcgctcga gactagaaat gcgcccgcgt tctcgaatcg
       61 tttggtgggc ttccggtgcg tcacgccccg atagagggat cagcggcgct cgattcgatg
      121 gcaattggag ctgattgtgg atgaggtacc ggagcgcgac gtgagtctga gcggcgctct
      181 catcgacggc cgctaccgcg tcgagtactg catcggcagc ggcgggatgg gcgtggtttg
      241 gcgagtgcga cacgtgcgct cgcgtcaagc atttgcgctc aagactctgc ggacgcgcgc
      301 cacacgagat cgcaaagcgc tgcgacggat tttgcaggag gcccgcgccg cagccgcgat
      361 tcgctcgcgc cacgtagtgc gtgtcgtcga cgtgaagccc gactacgtac atgaagggac
      421 ggcgctgccg ttcatcgtaa tggaactgct ggagggaacg agcctcgccg actacctcgc
      481 cagcgtggac cggctcagcg agggacaagt ggtttgggtc atgcgcgcgg tgagccgcgg
      541 cctgagtcgg gcccacgagc aaggcatcat tcaccgcgac ctgaagccgt ccaacatctt
      601 tctcgcccga gacgacgacg acggcgtggt cgtcaagttg tgtgacttcg gcatcgccaa
      661 actacacgcc agcgcccact cggaggctag cgagccactg accttgtcaa ccgatacggg
      721 cgtgattttg ggcaccccgc gctacatggc acccgaacag ctgcgacaaa gcggcagcga
      781 gcggccggct acggatcagt gggccttcgc gctggtcgcg tttcgagcgc ttacgggccg
      841 aggttacttc gagcaagccc gcagcatggc cgagttgctc ttggcgatcg tccacgatgc
      901 ccttccgaag ccgtcacggg tttcgcacct cggcgccgaa ttcgacgctt ggttcgagcg
      961 ctcgtgcgct cgtgatccag agcaacgttt cgccagcgtg gaagcgcagc agcgcgcgct
     1021 ggaacaagcg ctcggcacgc ccgaagcgac tcccatcgcg cccgctgact tgcaggggtc
     1081 ggaagccgct aacgaacccg gcacgcgttc cgcctcgccg tcgaaagagg agccgccgcg
     1141 agcacatctt cgcccctttt atgtggcact agtcgccgca actctgctct cgctcgtcag
     1201 cacaatccga gtcgcggcgg agttgaggcc caggcccccg ccgctgcgcg agatcgcgca
     1261 agtctcgacc gtttcgagcg cgcccgcccg tgggcgcgcg gcgatgacgc ctattgagtc
     1321 gacgagcgag atcgcgaagc cggcgccagc cgttgccctc gacgacaccg ccaaggccga
     1381 aagcaccaaa ccagtttccc ccgaaccgcc gcccaagccg ctgcgccgtc gcggcaagcc
     1441 tcgcgcagtc tcggcgatgt cgccgctgct tcctctcggc gcgccttgcg cgcgttcctc
     1501 cgagtgccga gacggcctgt gcgccgcaga ggtttgtcaa tgagacgagg gttggtcggc
     1561 ttcgcgctcg ccgctgcgtc ttcgctcttc tgctttcagg cgagcggcga cgacgcgcgc
     1621 gccgcgatga cacgagccat cgccgccaag gagcgcgcga tcgataccgc cgagcgcagc
     1681 gattggatcc gtgccttgga gctgatgcag gcggcggacc ggctgtcgag taccgcggtg
     1741 tcgaaatacg agctcggttt cgttgcagcc caactcggcg aaatcgcgct cgcggtagag
     1801 tgctatgaag cagccctgtc gctcgacttg caagggagcg cggccgagaa ggcgcgcgca
     1861 ttcctggaac gacagcggca agcttcggcc gagtcgactg caaccgagca accgcgcgcg
     1921 gtcacgccgt cgagcgtgcc acctcccgac ccagtctcac ggaccgaccc ggcgacgcaa
     1981 ccgcgcgccg agcgcccgaa gcgccacgac gcgcccttgc tcgcaccgct ccagcgtccc
     2041 acagaacccg cctcgcacac cgcgaagtgg gtgctcttgg gctctggtgc aaccctgagc
     2101 attctggcgc ttggattcgt tccaataacg gagcgtcagc tggcgcgccg ccgagaggtg
     2161 ttgcgctccg cttgtgcgct ccagaccgac ggacccgata cgtgtgccca cgccaaacca
     2221 ggccagtggg agaccgccca atccgcgtct gattcgattg ccctgtggaa gacggcgcgc
     2281 aacctgtctt gggtcggctt gggcgtcggc gtgtccgttc tcgcggcgga tggcctcgcg
     2341 cttctgcttt cggcaccgac tgcgcgttcc ggcgc
//
LOCUS       DASTKS010000869         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1926643, whole genome shotgun sequence.
ACCESSION   DASTKS010000869 DASTKS010000000
VERSION     DASTKS010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926643"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFQ35_18085"
     CDS             <1..210
                     /locus_tag="VFQ35_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932621.1"
                     /translation="SMATATAELSSARSEVELGIDSATSALADKAAAVESKLAEQSAE
                     LDGLADGLDQKVAAAKTALASVLED"
     gene            257..1204
                     /locus_tag="VFQ35_18090"
     CDS             257..1204
                     /locus_tag="VFQ35_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932622.1"
                     /translation="MRAATGAVAREIAALKAMAAKTPKTSKAVAAELSAAQSGLALVE
                     KSLLDPVATARKALDTTSAGLTDSITTVSRGLDALQSQCIVQIQGILEQFDAAVDAAK
                     TTIESVRVTVKELSNTVLPPIKALNSLLQQVQSAFKLGVQALQTCLNSAMSAVDAIPV
                     SALPKPLVASTQAAINKAMSAAATAIGAAGEQASTQITTAVSSVVTQIDTAKTAAVNQ
                     LNALVAPLTKQLDAIMPAVLAQLSTARQAILSLMNQVASQIDGAVSAALATANQTKTQ
                     MSQTVTSVVTRISASIDTELASVSASVRACRVTLSKPSS"
     gene            1396..2028
                     /locus_tag="VFQ35_18095"
     CDS             1396..2028
                     /locus_tag="VFQ35_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932623.1"
                     /translation="MGTSNDSAIELNGLNFDCDWRLGFNLDPARKGTIGYILFWSGCG
                     GLSLSKDIEVWSPFSGSGQTVVSGPTVKCVGLIESFRYSGDDDAPIRIRALVSKDTAA
                     NVRAKLASPVTATTLQLSWYIVSFDEDRKQWFEAAFVKNGARAQAAVDVMQGTLQLFI
                     DSKAIPVGEHLDIGVYRLEFQVVPTEGPGSLLEFATGPTQRLVKKWAADD"
     gene            2112..>2708
                     /gene="tssB"
                     /locus_tag="VFQ35_18100"
     CDS             2112..>2708
                     /gene="tssB"
                     /locus_tag="VFQ35_18100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HET9932624.1"
                     /translation="MAITDEVPRSRITLTYRTTVRGEPEDVTLPFRVLIMGDLSRGSS
                     KDRKLELDKRQIRRLDGKNLNQVMRDMGMSLKFNVKNRINPKSGGEEFEVQLPVESAR
                     AFQPAEVAHQVPRIKALLLLKKLLLEAQANFDNRKEFRNLLREAAKSSASIDALVKEL
                     PTFETFKLPAAKLQVTVPDDILKLPKLEIRRAMLVPDPL"
BASE COUNT          545 a          823 c          884 g          456 t
ORIGIN      
        1 tccatggcga cggcgacggc cgagctttcc tcggcgcgga gcgaagtcga gctcgggatc
       61 gactcggcga cgagcgccct cgcagacaag gcagcggccg tcgaatcgaa gctcgcggag
      121 caatcggcgg aactcgacgg cctcgccgac ggtttggacc agaaggtcgc ggccgccaag
      181 acagcgctcg cgtcggtgtt ggaggattga gatgttttcg gcggagcgac agcagctgac
      241 tgcattggaa ggtcgggtgc gagctgcgac gggcgcggtt gcgcgcgaga tcgcggcgtt
      301 gaaagccatg gccgcgaaga ccccgaagac cagcaaagcc gtcgccgccg agctcagtgc
      361 agcccagagc gggctcgctc tcgtcgaaaa gtcgctgctc gatcccgtag cgacggcgcg
      421 caaggccctc gacaccacat ccgccgggct caccgactcg atcacgacgg tgtcgcgcgg
      481 cctcgatgcg ctacagagcc aatgcatcgt ccagatccaa gggatcctgg agcagttcga
      541 tgcggcggtg gacgcggcga aaaccaccat cgagtcggtg cgcgtcaccg tcaaagagtt
      601 gtcgaatacg gtgctgcctc cgatcaaggc gctaaactcg ctgcttcagc aggtacaaag
      661 cgcgttcaag ctcggcgtcc aagcgctgca gacttgtctg aacagcgcga tgagcgcagt
      721 agacgcgatt ccggtctctg cattgccgaa gccactcgtg gcgtcgaccc aagccgcgat
      781 caacaaggcg atgtctgccg ccgccacggc gataggcgcc gcgggcgagc aagcatctac
      841 ccaaataacg accgcggtct cgagcgtcgt cactcagatc gacactgcga agacggccgc
      901 ggtgaatcag ctgaacgcgt tggtcgcgcc gctcacgaag cagctcgacg cgatcatgcc
      961 cgccgtcctc gctcagctct cgacggcgcg gcaggccatc ctgtcgctga tgaatcaggt
     1021 cgcttcgcag atcgacggcg cggtttccgc cgcgctcgcg accgcaaacc agacgaagac
     1081 gcagatgtcg caaaccgtca cgagcgtcgt cacgcgcatc tccgcgagca tcgacacgga
     1141 actcgcttcg gtctcggcga gcgttcgggc gtgtcgggtg acgctgtcga agccgtcgtc
     1201 gtagctagtc gcttgtccgc gagcgcgcgt tcgtggcacc ctgcggtatt gaatcgtgag
     1261 tcgcgcatgg agcgcgctca cacactcgag tgaacgtcca gcgtgatcgc gcctgaccga
     1321 gcaagcaggt aatcgagagc gctggcggga gccgacgcat gccgttcgct ggcagcgcgt
     1381 cgcggggacg cctgcatggg tacgagcaac gacagcgcga tcgagctcaa cgggctcaac
     1441 ttcgattgcg attggcgact gggcttcaac ctggacccag cgcggaaggg caccatcggc
     1501 tacatcttgt tttggagcgg gtgcggtggc ttgagcctgt cgaaggacat cgaggtttgg
     1561 agtccgttct cgggcagcgg tcaaacggtg gtgtcgggtc ccaccgtcaa atgcgtcgga
     1621 ttgatcgaga gcttccgcta ctccggtgac gacgacgcac cgattcggat ccgggcgttg
     1681 gtcagcaaag acaccgcggc aaatgtgcgc gcaaaattgg ctagtccagt cacagcgact
     1741 actctgcagc tcagctggta catcgtctcg ttcgacgagg acaggaagca atggttcgaa
     1801 gctgcgttcg tcaagaacgg cgcgcgcgcg caggccgcgg tcgatgtgat gcagggcacg
     1861 ctacagctct tcatcgacag caaggcgatt cccgtcggcg agcacctcga tatcggcgtg
     1921 taccgactcg agtttcaggt cgtgccgacc gaaggcccgg gtagcctctt ggagttcgcc
     1981 accgggccga ctcagcggct cgtcaaaaag tgggcggcgg acgactgagc accacgcgcg
     2041 agtaactcat cgacggccct ccttgcgcgc ggaagcaaga ggcgagtgca tccaacggaa
     2101 acggagaaaa catggcgata acagacgagg tccccaggtc gagaattacg ctcacgtacc
     2161 gcacgacggt gcgcggcgag cccgaggacg tgacgctgcc atttcgggtc ttgatcatgg
     2221 gagatctgtc gcgtggcagc tcgaaggatc gcaagctcga gctggacaag cgtcagattc
     2281 ggcggctcga cggcaagaat ctgaatcagg tgatgaggga catgggcatg tctctcaaat
     2341 tcaacgtgaa gaatcggatc aacccgaaga gcggtgggga agagttcgag gtgcagctcc
     2401 cggtcgagtc tgcgcgagcg tttcagcccg cggaggtcgc gcatcaggtg ccacgcatca
     2461 aggcgctctt gctgttgaag aagctcttgc tcgaggctca ggccaatttc gataatcgga
     2521 aggagttccg caacctgctc cgcgaagccg ccaagagctc ggcctcgatc gacgcgctcg
     2581 tcaaggagct gccgaccttc gagaccttca agctgcctgc ggccaagttg caggtgaccg
     2641 tgcccgacga cattctgaag ttgccgaagc tcgaaattcg gcgcgccatg ttggttccgg
     2701 atccactc
//
LOCUS       DASTKS010000870         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1031685, whole genome shotgun sequence.
ACCESSION   DASTKS010000870 DASTKS010000000
VERSION     DASTKS010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031685"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VFQ35_18105"
     CDS             complement(<1..1423)
                     /locus_tag="VFQ35_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9932625.1"
                     /translation="MTDTQTVTQNTPLSGFAALFEAGGEKADIGSEGEIVSGIIVQVT
                     RDSVVVDIGGKSEGVIARDEFLDAHGNLGVKAGDRVDVFVESRESDDGLISLSKEKAD
                     KMKVWDEISSACERDEIIEGTISQRVKGGLSVTIRGGVKAFLPGSQVDLRPIRNLEKL
                     IGQTYEFKVIKFNKKRGNIVLSRRALLERERDEMKAKTLQTLEEGMVVTGTIKNLTEY
                     GAFVDLGGIDGLLHITDMSWGRVNHPGEVFKVGDEVTVKVLKYNAETERVSLGLKQTQ
                     EDPWNHAEEAYPLGKRVRGKVMSLTDYGAFIELEPGVEGLIHVSEMSWTKKVKHPSKL
                     LEMGQEVECQVLEVDAKAKRISLGLKQLEPDPWSLFTDKYKPGDKVSGKVRSITDYGV
                     FVGIEEGVDGMVHKTDLSWTVKINNPADVYNKGDDVEAIILSINHDEKKVSLGIKQLY
                     EDPWNRIPGDYPAGTMLEVRVISI"
     gene            complement(1606..2487)
                     /locus_tag="VFQ35_18110"
     CDS             complement(1606..2487)
                     /locus_tag="VFQ35_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932626.1"
                     /translation="MVDASPRRGRSFRIVALVFVALLAVGLVAAFVVYKKYVAYEPTV
                     AAHVPSSARAAVRVDLTHVMFYEPFRRSMFPLADRFAKGQSSRRERLEARGIRVNSDI
                     REVLAVSGPGPGDWALVLGGRMPRGSTSSGVAAVLKEEGRELTEKEGVYTLAETGLAF
                     AESADGAFALASSVNTLASVLPAGVSNPVLSSGSGGLLLRPGTISAAPAGVTATFRAG
                     SVVEIRGRAELENSERAEADIRSLLGLLAGSDAAAMGAIRSAEFSKEASGLGFRVALP
                     REAVLGIIEALAQRVPG"
     gene            complement(2525..>3111)
                     /locus_tag="VFQ35_18115"
     CDS             complement(2525..>3111)
                     /locus_tag="VFQ35_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932627.1"
                     /translation="GDFHDTVPSSLGGTPRWSGARDLGGGLFRFEREPPRLRAAPAVI
                     YWRVPDVVIIGSEAEIDALERTVEKGALDPSPHAPEVGLVAVAARLGTLRAKLSQRAP
                     TLASYVEGAERLEGSLDRQGELFKLRVDVRFQSESQVKRLSGDLRSLQQKLRDEGHAW
                     VDKFSFEPLGEDLAVRLELGASELAALIRTFSRS"
BASE COUNT          543 a         1030 c          973 g          565 t
ORIGIN      
        1 tgatcgagat cacgcgcacc tcgagcatcg tgcccgccgg gtagtcaccc gggatgcggt
       61 tccacggatc ctcgtagagc tgcttgatgc cgagcgagac cttcttctcg tcgtggttga
      121 tcgagaggat gatcgcctcg acatcgtcgc ccttgttgta gacgtcggcc gggttgttga
      181 tcttgaccgt ccaggagagg tcggtcttgt gcaccatgcc gtccacgccc tcttcgattc
      241 cgacgaacac gccgtaatcg gtgatggagc ggaccttgcc ggagaccttg tcgcctggct
      301 tgtacttatc ggtgaagagg ctccagggat cgggctcgag ctgcttgaga ccgagcgaga
      361 tgcgcttggc cttggcgtcg acctcgagaa cttggcactc gacctcttga cccatctcga
      421 ggagcttcga cggatgcttg accttcttgg tccagctcat ctcgctcacg tggatgagac
      481 cttcgacgcc cggctcgagc tcgatgaacg cgccgtaatc ggtgaggctc atcaccttgc
      541 cgcggacgcg cttgccgagc ggatacgcct cttcggcgtg gttccacgga tcttcctggg
      601 tctgcttcag gccgaggctg acgcgctcgg tctcggcgtt gtacttcagc accttgacgg
      661 tgacttcgtc gccgaccttg aacacctcgc cggggtggtt cacgcgaccc caggacatgt
      721 cggtgatgtg gagcaggccg tcgatgccgc cgaggtcgac gaacgcgccg tactcggtga
      781 ggttcttgat ggtaccggtg accaccatgc cctcctcgag ggtctgcagc gtcttcgcct
      841 tcatctcgtc gcgctcgcgc tcgagcaggg cgcgccgcga aagcacgatg ttgccgcgct
      901 tcttgttgaa cttgatgacc ttgaattcgt aggtctgacc gatgagcttc tcgaggttgc
      961 ggatgggccg cagatccacc tgagagccgg ggaggaaggc ctttacgccg ccgcgaatgg
     1021 tgaccgagag gccgcccttc acgcgctggc tgatggtgcc ttcgatgatc tcgtcgcgct
     1081 cgcacgcgct cgagatctcg tcccacacct tcatcttgtc ggccttctcc ttggagaggg
     1141 agatgaggcc gtcgtcgctc tcgcgactct caacgaacac gtctacgcgg tcgcccgctt
     1201 tgacacccag gtttccgtgc gcgtcgagga actcgtcccg cgcgataaca ccttcgctct
     1261 tgcctccgat gtccacgacg acggagtcac gcgtcacctg tacaatgatg cccgagacga
     1321 tttcgccttc gctgccgatg tctgcctttt ctccgcctgc ctcgaacaag gcagcgaaac
     1381 ccgacagcgg ggtgttttgg gtgacggttt gggtgtcagt catggaagga aacgttgcct
     1441 ccagtcgcgg gggaccagtg cgcgagactc agctggctgc aagatgcacc ggtgtcggcc
     1501 tcgccaatac gaaggcgctc aggtagcgca cccgccctct cgagtcaaac gtgaactacg
     1561 aggcgggtaa cgcggtggag ctcggaccct gacgccgagc gtcgctcagc cgggtacgcg
     1621 ctgagccagc gcctcgataa tgcctagcac cgcctctcgg ggtaacgcca cccgaaaacc
     1681 aagtccagac gcctctttgc tgaactctgc ggaccggatc gctcccatcg ctgcggcgtc
     1741 gctcccggcg agcagaccaa gcagtgagcg gatatccgcc tcggcgcgct cgctgttttc
     1801 gagctccgcg cgaccccgaa tttcgaccac cgaccccgcg cgaaacgtgg ccgtaacccc
     1861 tgccggcgcg gccgagatcg ttccgggccg cagcaggaga ccccccgaac cggagctcag
     1921 caccgggttc gagacaccgg ccggaagaac gctcgccagc gtatttacgg acgacgcgag
     1981 ggcgaaggcc ccgtcggcgc tctctgcgaa cgcaaggccg gtttcggcaa gggtgtacac
     2041 gccctctttt tcggtcagct cccttccctc ctctttcagg acggcggcga cgccggagct
     2101 ggtcgagccg cgtggcattc ggcctccgag aacgagtgcc caatcgccgg gccccggacc
     2161 cgacaccgcg agcacctcgc gaatatccga gttgacgcga atgccccgcg cttcgaggcg
     2221 ctctcgccga gagctctgcc ccttcgcgaa gcgatcggcg agggggaaca tcgagcgacg
     2281 aaacggctcg tagaacatca cgtgggtcag atcgacgcgc accgccgccc gcgcactcga
     2341 ggggacatga gcggcgacgg tcggctcgta agccacgtac ttcttgtaca cgacgaaggc
     2401 ggccacgagc cccaccgcga gcagagcgac gaagacgagc gcaacgatcc gaaaagagcg
     2461 tccgcgacgg ggcgaagcat cgaccatcac ggcgcgcaaa ctaccaccag acccgaaaag
     2521 acgctcagct tctcgagaag gttcggatca gcgcggcaag ctcgctcgcg ccgagttcga
     2581 gccggaccgc gagatcttcg ccgaggggct cgaagctgaa cttgtcgacc catgcgtggc
     2641 cctcgtcacg cagcttttgc tgcaagcttc gaagatcccc agacagcctt ttcacctgag
     2701 attcgctttg aaagcgaacg tcgacgcgga gcttgaagag ctcaccctgg cgatccaggc
     2761 tgccttcgag ccgctcggcg ccttcgacgt agctcgcgag cgtgggcgcg cgctggctca
     2821 gcttggcgcg cagcgtcccg agccgcgccg cgacggccac gagcccgact tcgggagcgt
     2881 gcggagaagg atccagtgcg cccttctcta cggtgcgctc gagcgcatcg atttctgctt
     2941 cggagccgat gatgacgacg tcggggacgc gccaataaat gacggcggga gccgcgcgga
     3001 ggcgaggcgg ctcccgctcg aatcgaaaca gccctcctcc gagatcgcgc gcgccgctcc
     3061 agcgtggcgt cccaccgagc gaggacggaa ccgtatcgtg aaagtcgccg c
//
LOCUS       DASTKS010000871         8470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_808476, whole genome shotgun sequence.
ACCESSION   DASTKS010000871 DASTKS010000000
VERSION     DASTKS010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8470
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808476"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFQ35_18120"
     CDS             complement(<1..803)
                     /locus_tag="VFQ35_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932628.1"
                     /translation="MKALNNLSYLFLCVPVLVAACTIETTGGSPSGEGGAAALGGKGG
                     TAGAGGASTSSGGRGTTSEGGKTSAEGGSDANSTGGNIGGKPSGSGGKPSGTGGKASG
                     SGGKPAEPPPVSGAAGNGDGLPEAGAPGDDPPATSPERFFFPTPNATNTVAPKVVVDA
                     QGGVHAAYPAYFGGRAYYAYCKSNCSSATAFQVVELDVGSSIGNVSLALTPQGKPRLV
                     LSEFDKAYYAACDAQCGTLAGWTMTPVFDHDNSMEVSGQALAIDTAGRVR"
     gene            920..1411
                     /locus_tag="VFQ35_18125"
     CDS             920..1411
                     /locus_tag="VFQ35_18125"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9932629.1"
                     /translation="MVTASAVSPPLTEELVEYMATGVDMIVATRDAALAPESMLGMGI
                     KVHPDRCTVTAYLPTERAAATLRNLQDNGQIAITLVHASTGKGIQLKGSVVSLRESDS
                     SDRDLQAVFRSALVEELAAVGVPRSATRRLHWWPSTAVDVAVDSVFVQTPGPKAGEPF
                     KKA"
     gene            1417..2826
                     /locus_tag="VFQ35_18130"
     CDS             1417..2826
                     /locus_tag="VFQ35_18130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9932630.1"
                     /translation="MSQPTLQSLWRCLDGIAPAMFSTCSASGVPNVSMISHVKYVDPK
                     HVAISRQFFNKTTQNLAENPQALVVIWDPITFDHHRLRLRFLRSESEGPLFDSMSARI
                     QAIASHTGMSGIFRLLSADVFEVLSIETDIGVIAAESSEPAEGPDLEPSPVDVPPESR
                     SELWVLHRLAARMRCARDLDELLRSVLLSLSEDLGFSHSMVLLPDERGERLFTVASHG
                     YGDNGVGAEITFGQGLIGIVAQRRQLQRLSSLDPALRYGRAVRESVQAQGSHSVAPEI
                     PLPGLPNAQSQLVLPLLVRERLVGVLALESPKPHVFEAWHEAFLSVLSDQLAHALLLA
                     LERDDAEETARPPAPQPEVVSKPQPRNTRSFCLYKNDDCVFVDGEYLIRNVAARILWR
                     VLTEHAREGRTEFTNRELRLDPKLGLPSIRDNLESRLILLRRRLELKCPDVRLVSRGR
                     GRFALETSANLAIEERESA"
     gene            2989..4110
                     /locus_tag="VFQ35_18135"
     CDS             2989..4110
                     /locus_tag="VFQ35_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932631.1"
                     /translation="MELLRSVRSRALCLAALALAAGSLSVLRGARAERTAPPLLPAVK
                     SGVPEAPLPARLSSTGLYAADSVDAIAPDVMFYVPQYPLWSDGAKKRRWIRLPQGARI
                     DATNADAWAFPPGTRFWKEFSFGARVETRYMERLRDGSYRYATYVWDAEQRDAFLAPE
                     KGLNDVKPLGARASHDIPSNADCRACHEGRHSQVLGFNALQLSPDRDPMAPHRELATS
                     DAVDLPELSRRGLIQGLAPALLARPPRIEAPSKVARAAAGYLFANCAHCHNAESPLAV
                     LGLDFDQSVLDSAGGFHLFERLAGRATHFRLTNEQSSVALAPGHPEKSAILARAASRN
                     AAQQMPPLGTELVDTEGTELLQRWITQSSFSKDRKENEP"
     gene            4107..4769
                     /locus_tag="VFQ35_18140"
     CDS             4107..4769
                     /locus_tag="VFQ35_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932632.1"
                     /translation="MKNRLIATTVLGSCLLASGVLWLSSRVATASPNAPRVQAPSVAA
                     SPPAQDASAKSRAVALGEHLVLTAGCHDCHTPLKVGDHGPEPDMSRQLSGHPESIKLP
                     RSPAPSGPWIVSVSATNTAWSGPWGVSFTANLTSDKDTGLGSWSKDMFIQTIRTGRHM
                     GRGRPLLPPMPVPVYNNFSDEELGAIFAYLQTVPAIKNRVPDPLPPPAAAKPVTAKPS
                     SH"
     gene            complement(4811..5524)
                     /locus_tag="VFQ35_18145"
     CDS             complement(4811..5524)
                     /locus_tag="VFQ35_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9932633.1"
                     /translation="MAASEHQHAWVIGGSGAVGSAVVRELRARGLTCTFTWYENEAAA
                     HELARATGARPLRFAAECSTEIEAWVEGALRDHGAPRALVYAAGTSAALKLDAIDVDV
                     FERALKVNVTAPFLVLRALAKRHAPFDAVLVGALDRGQSLPLPVHYATTQGALGAMTM
                     AIARELGPLGVRVNQVALGVLEDGLSRTLDAAVVQQYVKFSALRRRGTSSEVARAIAW
                     LLLENSYVTGKVVPVNGGI"
     gene            complement(5511..6257)
                     /locus_tag="VFQ35_18150"
     CDS             complement(5511..6257)
                     /locus_tag="VFQ35_18150"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9932634.1"
                     /translation="MTKPRLQGDVCLVTGGSRGLGKAMSLAFAREGAKVALTYRQNTA
                     EAEQTLELLKSLGAEVLMFQGDVADSAHVQDTVKAVLAAWGKIDVLVNNAALTQVVPL
                     AMLEEDEWDEVVSTTLKGAYLFSRAVLRGMIRKKRGHILNIGAFSSERMVESPLHFAA
                     AKSGLRGMTEALALDVGKHNIMVNLLAPGLLDVGLGQMLLPHRLNEYREQCALGRVGS
                     AAEVAELAVFLVSSENTFITGAKLVADGGL"
     gene            complement(6254..7729)
                     /locus_tag="VFQ35_18155"
     CDS             complement(6254..7729)
                     /locus_tag="VFQ35_18155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9932635.1"
                     /translation="MKALYRTTSLCRSCKNALPAEVVSSAEGEVFLRKTCPEHGDQEV
                     KLSTDVDWYLRTRAEEPRLVAPRRERHEVKHGCPFDCGPCEQHEQTVRLPVVTITSAC
                     NLDCPICYVHNKNKDPFHMPVSDFERLLTHLKREHGEIDIVNFTGGEPLLHPEFAEFL
                     RLSGEAGVHRVTICSNGIRLARDEELVKRLADAGARVALSFDTFDRAVDYELQGAKLL
                     DLKLRCLELLEKHAVDTTLIPVMTKGLNDHEIGRILELAFKTRCVRHVEVHTITFTGQ
                     GGTSFSRERRISMVEVLDAIAATTGGLLGRDDFVSSPCAHPLCYQIAYLLLDPEGGPP
                     IPFSRFLPRETISECLADRLYMEPSRKLERALLEAIDRLWASESGESERTLRILKRLL
                     KDLFPRDRVMTQREALRISEVASKAVYVHSHMDEETFDTERIVSCCDSNCYADGSTIP
                     VCAYNVLYRDKEEHFMLKPMTWSARSGGQKSLRAGRLPVLP"
     gene            complement(7726..>8470)
                     /locus_tag="VFQ35_18160"
     CDS             complement(7726..>8470)
                     /locus_tag="VFQ35_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9932636.1"
                     /translation="DVAALFVEPIQGEGGVRPLSAEFIAAACELTHRMGALLVADEIQ
                     TGFGRSGAFLRSASWPRRPDVVILGKQLGGGLMPLSAMMTRRELFERAYGEDFEDGEA
                     HNMTMGYNALSAVAGLAALDLLSEDLVATVRERGARLSTKLASALSENSLFAEVRGSG
                     YMLGIELRQPDHPWLSFEQFGMPAWDGRPLIAPLLCARLYRRGYYCFPCGHDWRVVRI
                     QPRFDITEGELGEFVETLREELARVEEVA"
BASE COUNT         1429 a         2797 c         2698 g         1546 t
ORIGIN      
        1 cgcacgcgcc cggccgtgtc gatcgccagc gcctgcccac tcacctccat cgagttgtcg
       61 tggtcgaaca cgggcgtcat cgtccaaccg gctagcgtcc cacattgagc gtcgcacgcg
      121 gcgtagtacg ctttgtcgaa ctccgagagc acgagtcgcg gcttcccctg cggcgtgagg
      181 gcgagcgaaa catttccgat gctgctccca acgtcgagct cgacgacttg gaaggccgtt
      241 gcgctcgagc aattcgactt acagtaagcg tagtaagctc gtccaccgaa gtaagcggga
      301 tacgctgcgt gcactccgcc ctgcgcgtcg acgacgacct tgggcgcgac ggtgttcgtt
      361 gcgttgggag tcgggaagaa gaaccgctcg gggctcgttg cgggagggtc gtcgccgggg
      421 gcgcccgcct ccggcaggcc atctccgttt ccggcggctc cgctgacggg cggcggttca
      481 gcaggcttcc cacccgagcc gctcgctttt cccccggttc cactcggttt tccgccggag
      541 ccggacggct ttccgccgat gtttcccccg gtcgaattgg cgtcgctgcc gccctccgcc
      601 gacgtcttcc cgccttcgga cgtcgttccg cgtccgccac tcgatgtcga ggctccgccc
      661 gcgcctgcag tgccgccttt tccgccgaga gctgccgctc cgccctctcc ggagggtgag
      721 ccgcccgtcg tctcgatcgt gcacgcggcg acgagcacgg ggacgcagag gaacaggtac
      781 gagaggttgt tcaatgcttt cataccctgt tcatgtgtcg cgaactcgcc ggtcgttact
      841 aagagctcct tggggacccg ctaatggaat gctaaaaagt cgctaaccgc ttggtctcga
      901 aacaggtaag ttcgcagaaa tggtaactgc gtccgccgtc tctccgccgc tcaccgagga
      961 gctcgtcgag tacatggcga ccggcgtgga catgatcgtc gccacgcgtg acgcggcgct
     1021 cgcacccgaa tcgatgctgg ggatgggcat caaggtgcac ccagaccgtt gcacggtgac
     1081 ggcgtacctt ccgaccgagc gcgcggccgc caccctccgc aacctgcaag acaacggcca
     1141 gattgcgatt accctggtgc acgccagcac gggcaaggga attcagctca agggaagcgt
     1201 cgtctcgctt cgtgagagcg actcatcgga tcgcgatctt caggcggttt ttcggtctgc
     1261 gctggtcgag gagttggcgg cggtcggcgt tccgcggtcg gcgacgcggc ggctgcattg
     1321 gtggcccagc accgccgtgg acgtcgcggt ggattcggtc ttcgttcaaa caccgggccc
     1381 caaagccggt gagccgttca agaaagcctg agccgcatgt ctcagcccac gttacagtca
     1441 ctctggcgct gcctcgacgg catcgctccg gccatgtttt ccacgtgttc ggcgagcggc
     1501 gtgccgaacg tgagcatgat cagccacgtc aaatacgtgg atccgaagca tgtcgcgata
     1561 tctcgtcagt tcttcaacaa gacgacgcag aacctcgccg agaacccgca agcgctggtc
     1621 gtgatttggg atccgatcac cttcgaccat catcggttgc gactacggtt cctccggtcg
     1681 gagtccgaag ggccgctctt cgattcgatg tcggcgcgca tccaggcgat cgcgtcccac
     1741 accggtatgt ccggcatttt caggctgctt tcggcggacg tcttcgaggt gctctcgatc
     1801 gagaccgaca tcggcgtcat cgccgccgag tcatcggagc ccgctgaagg acccgacctc
     1861 gagccgtctc cggtcgacgt tccgcccgag tcgcgcagcg agctctgggt gcttcatcgc
     1921 ttggcggcgc ggatgcgttg tgcccgcgac ctcgacgagc tcttgcgttc ggtgctgctc
     1981 tcgctctcgg aagatctcgg gttttcgcac agcatggtgc tcttgccgga cgagcgcggg
     2041 gaacgcttgt tcaccgttgc gagccacggc tacggcgaca acggggtcgg agcggaaatc
     2101 acgttcggtc aggggctcat cggcatcgtg gcccagcgcc ggcagctcca gcgcctgagt
     2161 tcgctcgatc cggcgctgcg ctatgggcgt gccgtgcgcg agagcgtgca ggctcaagga
     2221 agtcacagcg tcgcgccaga aattccgctg ccggggctac caaacgcaca gagccagctg
     2281 gttctgccgt tgctcgtccg agagcggctc gtcggggtct tggcgctcga aagcccgaag
     2341 ccgcacgtgt tcgaagcgtg gcacgaagcc tttttgtccg tgctttcgga tcagctcgcg
     2401 cacgcgctgc tcttggcgct ggagcgagac gacgccgagg agacggctcg tccccccgcg
     2461 ccgcaacccg aagtcgtgag caagccgcag ccccgcaaca cgcgttcgtt ttgtctctac
     2521 aagaacgacg attgtgtttt cgtcgacggc gagtacctga ttcgtaacgt cgcggcgcga
     2581 attctatggc gggtgctcac cgagcacgca cgcgaagggc gcaccgagtt cacgaatcgt
     2641 gagctccgcc tggacccgaa gctcggtctg ccgtcgatcc gcgacaactt ggagagtcgt
     2701 ctcatcttgt tgcggcgacg gctcgagttg aagtgcccgg acgtgcggtt ggtctcgcgc
     2761 gggcgcggtc gcttcgcctt ggaaaccagc gcgaacctgg cgatagaaga gcgcgaaagc
     2821 gcctgaaaac ggggccgcaa cgcgcccatc tgccgcagca agaggatcgg ctcgcgcagg
     2881 atgccttacc ccattcgcgg gcccttagtg tctatttagc aatccgctaa ggttccgaac
     2941 gggacatctt agcaacggag aaagttcgga tgcggcattc atcgagtgat ggaactgctt
     3001 cgaagcgttc gctcccgtgc tctctgcctc gccgcgctcg cgctggccgc gggaagcctg
     3061 agtgtgcttc gcggcgcacg cgccgaacga acggctccgc ccctccttcc ggccgtgaag
     3121 agtggcgtac cggaggctcc cctcccggct cggctctctt cgactggact ttacgcagcc
     3181 gattccgtcg acgcgatcgc gcccgacgtg atgttctacg tgccgcagta tccactttgg
     3241 tcggacggcg ccaaaaagcg acgctggatt cgcttgcccc aaggcgcgcg catcgacgcg
     3301 accaacgcgg acgcgtgggc gtttccgccc ggcacgcgct tttggaagga gttcagcttt
     3361 ggcgcacgcg tcgagacgcg ttacatggag cggctccgtg acggcagcta ccggtacgcg
     3421 acgtacgttt gggatgcgga gcagcgcgac gccttcttgg cccccgaaaa agggctgaac
     3481 gacgtcaaac ccctcggcgc gcgggcgagc cacgacattc cttcgaacgc cgattgtcgc
     3541 gcttgtcacg agggtcgcca ctcgcaagtt ttgggcttca acgcgcttca gctctcgccc
     3601 gaccgcgacc ctatggctcc gcatcgcgag ctcgcaacga gcgacgccgt cgacctgccc
     3661 gagctttcgc ggcgcgggct gattcaggga ctcgcgcctg ctttgctcgc acgtccgccg
     3721 cggatcgagg caccttcgaa agtcgcgcgt gcggcggccg gctacctctt cgccaattgc
     3781 gcgcattgcc acaacgcgga gagcccgctc gcggtgctgg ggctcgactt cgaccaatcg
     3841 gtgctcgact ccgccggtgg cttccacctc ttcgaacgtc tcgcggggcg cgcgacgcac
     3901 ttccgtttga cgaatgagca gagctccgtc gcgttggcgc ctggtcaccc cgaaaagagc
     3961 gccattctcg ctcgtgcagc gtcgcgcaac gctgcgcagc agatgccacc gctgggcacc
     4021 gagctcgtcg ataccgaagg gaccgagctc cttcaacgct ggatcactca gagtagtttt
     4081 tcgaaggatc gaaaggaaaa cgagccatga agaatcgtct catcgcaacg actgtccttg
     4141 gtagctgcct gctggcgagc ggagtgcttt ggctttcgag tcgcgtcgct accgcttcgc
     4201 ccaacgctcc gcgagtgcaa gcgccgtcgg ttgcggcgtc accgccggcg caagacgcga
     4261 gcgcgaagag cagagcggtg gctctcggag agcacctggt cttgaccgct ggttgccacg
     4321 actgtcacac cccgctcaaa gtgggtgacc atggaccgga gccggacatg tcgcgtcagc
     4381 tctccggtca tccggaatcg atcaagctgc cgcgctcgcc cgcgccgagc ggcccctgga
     4441 ttgtgtcggt ctccgcgacg aatacggcgt ggtcgggccc gtggggcgtg agcttcacgg
     4501 cgaacctgac ctccgacaag gacactgggc tcggcagctg gagcaaggac atgttcattc
     4561 aaacgatccg caccggccgt cacatgggac gaggtcgccc gctactcccg ccgatgccag
     4621 tacccgttta caacaacttc agcgacgaag agctcggcgc gatcttcgcg tatttgcaga
     4681 ccgtgccagc cataaagaat cgcgtcccag acccgctgcc gccgccggct gccgcgaagc
     4741 ccgtgactgc gaagccatcg agccactgac gctcgactca cgggggctcc tctggagcga
     4801 tgtcgaatgg tcaaattccc ccgttcaccg gaacgacttt gcccgtgacg tagctgttct
     4861 cgagcaatag ccacgcgatt gcgcgggcga cttcgctcga ggtaccgcgg cgccgcagcg
     4921 cgctgaactt cacgtattgc tgcaccaccg cagcgtcgag cgttcgcgac aacccgtctt
     4981 cgagcacccc cagcgcgact tggttcactc gaacaccgag tggaccgagc tcgcgcgcga
     5041 tcgccatcgt catcgccccc aacgccccct gcgtcgtagc gtagtgcacc ggtaaaggca
     5101 gggattgccc gcggtcgagc gcgcccacca gcaccgcgtc gaagggtgcg tgtcgtttcg
     5161 ccagcgctcg taaaaccaag aatggcgccg tgacgttgac cttcaacgct cgttcgaaga
     5221 cgtcgacatc gatggcatct agcttcaagg ctgcgctcgt cccggctgca tagactaatg
     5281 cgcgcggagc gccgtggtct cggagcgcac cctcgaccca ggcctcgatt tcggtcgagc
     5341 actccgcagc gaagcgtagc ggtcgagctc cggtcgctcg cgcgagctcg tgcgcagcgg
     5401 cttcgttttc gtaccaggtg aacgtgcacg tgaggccacg agcccggagc tctcgaacca
     5461 ccgcgctgcc gacggcgccg ctcccgccga tgacccacgc gtgttggtgc tcagaggccg
     5521 ccatctgcca ccaactttgc gcccgtgatg aaggtgttct cggacgacac caggaagacg
     5581 gcgagctctg cgacctccgc cgccgaaccg acgcgcccga gcgcgcactg ctcgcgatac
     5641 tcgttcaagc ggtgaggcag aagcatttgc ccgagcccga cgtcgagcaa gcccggcgcg
     5701 agcaggttca ccatgatgtt gtgctttccg acgtcgagcg cgagtgcctc cgtcatgccg
     5761 cgcaaaccag acttggcggc cgcgaagtga agcggcgact cgaccatgcg ctccgaagag
     5821 aaggcgccga tgttcaagat gtggccgcgc ttcttgcgaa tcatgccgcg aagcaccgcg
     5881 cgcgagaaca gatacgcgcc tttcaacgtc gtcgagacga cctcgtccca ctcgtcttct
     5941 tcgagcatcg cgagcggaac gacctgggtg agggccgcgt tgttcaccaa gacgtcgatt
     6001 ttcccccaag ccgcgagcac tgctttgacg gtgtcctgga cgtgagcgga gtctgcgacg
     6061 tctccctgga acatcagcac ctccgccccg aggctcttca agagctcgag cgtttgctcg
     6121 gcctcggccg tgttttggcg gtaggtgagc gctactttgg cgccctctcg cgcgaacgcg
     6181 agcgacatgg ctttgccgag cccgcgcgac cctcccgtca ctaggcacac gtcgccttgg
     6241 agtcggggct tggtcacggc agcaccggta gcctgcccgc gcggagcgac ttctgtcctc
     6301 cgctgcgagc gctccacgtc atcggcttca gcatgaaatg ctcttccttg tcgcggtaga
     6361 gtacgttgta cgcgcacacc gggatcgtgc tgccgtctgc gtagcagttc gagtcgcaac
     6421 acgaaacgat gcgctcggtg tcgaaggtct cttcgtccat gtgggagtgg acgtagacgg
     6481 ctttggaggc aacctcgctg attcgcagcg cctcgcgctg ggtcatcacg cggtcgcgcg
     6541 ggaacaaatc tttgagcagg cgcttgagga ttcgcagcgt tcgctcgctt tcgccggact
     6601 cggacgccca caagcgatcg atcgcttcga gcagggcgcg ctcgagcttc cgcgaaggct
     6661 ccatgtagag ccggtccgcg aggcactcgg agatcgtctc gcgcggcaag aagcgagaaa
     6721 acgggatcgg ggggccgccc tcggggtcga gcagcaagta agcgatttgg taacagagcg
     6781 ggtgcgcaca cggtgacgaa acgaagtcgt cgcgccccaa caagccgccc gtggttgccg
     6841 cgatcgcatc gagtacctcg accatgctga tgcgccgctc tcgtgagaag ctcgtgccgc
     6901 cctgaccggt gaaagtgatc gtgtgcacct cgacgtgccg cacgcagcgc gtcttgaagg
     6961 cgagctccaa aatgcggccg atttcgtggt cgttgaggcc cttggtcatc accgggatga
     7021 gcgtcgtgtc caccgcgtgc ttctcgagca gctcgaggca gcgcagcttg aggtcgagaa
     7081 gcttcgcgcc ctggagttcg tagtcgaccg ccctgtcgaa cgtatcgaac gagagcgcga
     7141 cccgcgcgcc ggcgtccgcg agccgtttga ccagctcttc gtcgcgcgcc aggcgaatcc
     7201 cgttcgaaca gatggtgacg cggtgaacac cggcttcgcc cgagagccgg aggaactcgg
     7261 cgaactcggg gtggagcagc ggctcaccac cggtgaagtt cacgatgtcg atttcgccgt
     7321 gctcgcgctt caagtgggtg aggagacgct cgaagtcgct caccggcatg tgaaaggggt
     7381 ccttgttctt gttgtggacg tagcagatgg ggcagtcgag gttgcacgcg ctcgtgatgg
     7441 tgacgacggg taggcgcacc gtctgctcgt gctgctcgca agggccgcag tcgaatgggc
     7501 agccgtgctt cacctcgtgg cgttcgcggc ggggcgcgac caggcgcggc tcttcggcgc
     7561 gggtgcgtag gtaccaatcg acgtcggtcg agagcttcac ctcctggtcg ccatgttcgg
     7621 ggcaggtctt tcgcaggaac acttcgccct cggcgctgct cacgacctcc gccggcagcg
     7681 cgttcttgca gcttcggcag aggctcgtcg ttcgatagag cgctttcatg cgacctcttc
     7741 gacgcgcgcg agctcttcgc gcagcgtttc gacgaactcg ccgagctcgc cttcggtgat
     7801 gtcgaagcgc ggctgaatgc gcactacacg ccagtcgtgg ccgcagggga agcagtagta
     7861 gccccggcgg tagagccgag cgcagagcaa cggagcgatg agaggccgcc cgtcccacgc
     7921 cggcatgccg aactgctcga acgagagcca cgggtggtct ggttgccgga gctcgatgcc
     7981 cagcatgtag ccgctcccac gcacctcggc gaacagcgaa ttctcggaga gagcggaagc
     8041 gagcttcgtc gacaagcgag cgccccgttc tctcaccgtt gcgaccaggt cttcgctcaa
     8101 aaggtcgagc gcagcgagcc cggccacggc gctcagcgcg ttgtagccca tcgtcatgtt
     8161 gtgcgcttcg ccgtcctcga agtcttcgcc gtacgcccgc tcgaacagct cacggcgcgt
     8221 catcatcgcc gagaggggca tgagcccgcc gccgagctgc ttgccgagga tcacgacgtc
     8281 gggccggcgc ggccagctcg cgctgcggag aaatgcgccc gagcgcccga agccagtctg
     8341 aatttcgtct gccacgagca gcgctcccat tcgatgcgtg agctcgcacg cggccgcgat
     8401 gaactcggcc gagagcgggc gcacaccgcc ttcgccttga atcggctcca cgaagagggc
     8461 cgctacgtct
//
LOCUS       DASTKS010000872         9840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_362676, whole genome shotgun sequence.
ACCESSION   DASTKS010000872 DASTKS010000000
VERSION     DASTKS010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362676"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFQ35_18165"
     CDS             <1..317
                     /locus_tag="VFQ35_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932637.1"
                     /translation="VEAPALLFGSEPMLPAEHAAADTNTRRPANAFRVPNALIAVITV
                     AQSIVARKRTRGSRFDVSELDSNFERSNEIQSSRRAFKSVICNPGKAARLELGTIVCV
                     EP"
     gene            561..2123
                     /gene="proS"
                     /locus_tag="VFQ35_18170"
     CDS             561..2123
                     /gene="proS"
                     /locus_tag="VFQ35_18170"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9932638.1"
                     /translation="MAKNTPQKAAISPTRAEDYAQWYQEVVRAADLAETSPVRGCMVI
                     KPWGYAIWENIQRVLDGMFKATGHKNAYFPLFIPKSFLEKEAEHVEGFAKECAVVTHH
                     RLVAGKDGGLIPDPEAELEEPLIVRPTSETIIGAAFAQWVQSYRDLPLLINQWANVVR
                     WELRTRLFLRTAEFLWQEGHTAHATEAEAREETLKMLDVYATFAQDYMAMPVIRGPKT
                     ASERFPGAVDTYAIEAMMQDRKALQAGTSHFLGQNFAQASGIKFLDAEGQQRFAFTTS
                     WGVSTRLVGGLIMTHADDDGLVLPPRLAPAHAVIWPVFRGDDTRAAVLETIEKLSQSL
                     RAARYDDRPLQVETDLRDIGGGDKQWEWIKKGIPLRIEIGPRDVASGTAMVARRDRGP
                     KERESIPLAELPTRLPALLDEIQRSLYARALIVRDQATRPIDVRSEFDAFFTPTNAQK
                     PEIHGGFALSHWCGDPACEATIKDALKVTIRCIPMHGIDACGAATRLKEPGSCIVCNR
                     PSEQRVVFAKSY"
     gene            2276..3436
                     /locus_tag="VFQ35_18175"
     CDS             2276..3436
                     /locus_tag="VFQ35_18175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET9932639.1"
                     /translation="MLLGSISSRPLRILVVDDEATNLRVLSSYLKDANLEPTAVGDGR
                     AALDVFPSLAPDLVLLDLIMPELGGLDVLVGIRQHPALGETPVIVMTGFTDRQLRLEA
                     IEHGADDVLEKPLDPDVLLARVATLIKLKLSRDELKASHAALARQNDELERAQREQRE
                     LTEFIVHDLKGPLTGIVANTEWVYEQLSRQESGYLGALEDVLSSASRLRAMINDLMTV
                     SQLERGTFPVRRRTVAMGPLLRSVVRDFSRAAEQRQIELSAPHECEVDVEVDVDLIQR
                     VLGNILENSLRYTPERGKVALDAHLAQKLEIEIKNSGPPIPKFERDLIFEKFRRGSGA
                     GLTHGNAGLGLYFCKRAVQAHGGDIQVVETPDYPTCFLIRLPADSVNDASLA"
     gene            3893..5848
                     /locus_tag="VFQ35_18180"
     CDS             3893..5848
                     /locus_tag="VFQ35_18180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932640.1"
                     /translation="MVLVSPEIDAFLGRAIPSDSQPGVRFELERRLGVGGTAVAFLAS
                     RVSPEGQSPAVLKVILPAVAASHGSTAAMVVRKEAVALGRLNERVPPTPFVVRLMDTG
                     ALPYVFRGKHVDLPWLAIEFVDGGIEGTTLEERMDHTLNLTGKAFDRERAARAISHVC
                     AGLADVHAVGVIHRDLTPNNVLCCGFGDSELFKLSDFGIARPAGVTDTFGPSVIGTPG
                     YIAPEHLSDSASTTAGDIFALGCLVYFVLTGDHLFDVTNALELLASTQQNKRRSVRDA
                     SALSPELAGDDEACATIDAIIARATAHDPLRRPENPREIAAALAPLLLERKGSGSRRE
                     RATLMSAGQALGISRLSCLVRHPPGDERILRSVGWDGDGHCLAASTRGLEYWNGSEWR
                     VTETRDLPGGQDVRFVRRVGPGRWLLGGDHALLAEYSHSGVSRVMRGRDADASFLDAS
                     GNVADLAAVVAQRATGTIELCAAVGGRWLRPLPLPDAAFVSGLTQIDEERWLVVGRSR
                     QGGGFVLAYSPLSWSVEPVLAPPTRAWVACTARTERDVVLAVGTDGTVLRIERGIVDG
                     RVIAERPSLASVSVDVLDREWAGGVGELWCSIAGGDWTRVWHDATWNRPFVSLFADVS
                     SVVAMSVDGGVLECRPGLPSTPPGRAR"
     gene            5909..7042
                     /locus_tag="VFQ35_18185"
     CDS             5909..7042
                     /locus_tag="VFQ35_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HET9932641.1"
                     /translation="MQYFASCAKGTEGALRAELAELGLTGVRADRGGVHFGGERPAAF
                     RACLWSRIAVRILEPIGQFECPDEDALYGGVHGLDFTDVLSPKHTLSVSAAASNSRLT
                     HTQYIAQRTKDAIVDRQRDAFGERSNVERRDPDVHVFVHLSRDRATVYLDLAGSPLHL
                     RGYRRAHGDAPLKETLAAAVLRLSGFDPRRPVLDPMCGSGTLLIEAALWASGRAPGIF
                     RERFGFERWASHDARAKQVMKELREAARGSSDALPEFRGSDVDAAVLDSARENAERAG
                     VQLTLSHRSMIDVRPDGTNGLLVSNPPYGERLGANADLPRQLARLVDRFPDHSVGLLM
                     GAEQNLARTRRKPRMFSLFNGDIACTLRGYAPLDRSGTSRDSD"
     gene            7722..8213
                     /locus_tag="VFQ35_18190"
     CDS             7722..8213
                     /locus_tag="VFQ35_18190"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9932642.1"
                     /translation="MSAESGRNQRNLAEANAESRETSRPASTRQVVRRRSIRVARTTR
                     DQRAFALMRAHELEERIERPLVRRDCEDAPRPCPFVACRYHLFLDVSPSTGTIKFNFP
                     DLEVWELSETCALDVAAQGGHGHERTSELMNVTRERVRQIEAVALTKLKRAGRLRELE
                     VDR"
     gene            complement(8320..9015)
                     /locus_tag="VFQ35_18195"
     CDS             complement(8320..9015)
                     /locus_tag="VFQ35_18195"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9932643.1"
                     /translation="MSTIAVFLVWYLVFVFSTTCHEAAHAFAAKRGGDNTAYAMGQVT
                     LDPLPHIKREPFGMVIMPILSFQMGMMVGWASTPYDPHWGVRYPRRWALMSFAGPLTN
                     FLLALVALLALRALDSASVLHLSGHHGTSVGLVQLPPGQEFSSPLGALALALSALLKL
                     NVLLGVYNLIPVPPLDGAAVVQGFGPRQVSAFYDRIRMSPVAQIAGLLGAWWMFPIVF
                     TPVWRLVSALLYS"
BASE COUNT         1737 a         3309 c         3168 g         1626 t
ORIGIN      
        1 cggtcgaagc gcccgcgttg ttgtttggct ccgaaccaat gttgccggca gagcatgcgg
       61 cagcggacac caacaccaga aggccagcga acgccttccg cgttccgaat gcactcatcg
      121 cggttatcac tgttgctcaa agcatagttg cccgcaagcg cacgcgaggc tcacgattcg
      181 acgtcagcga gctcgattct aattttgaac gcagcaatga aatccaatcg agccgtcgag
      241 ctttcaaatc tgtaatctgc aaccccggca aagcggcgcg gctcgaactc ggtacgatag
      301 tgtgcgtcga gccctgacaa atcatccagc agggtgatta atcaagagtc ttgcccccgc
      361 ttcggagacg gaggtgggac cgagatctcg cctcgcgaca gggcgcaaaa ctcggggata
      421 ccccgggcga ttcgggcacg gctcgactcc gcgaaaggca ccgttcggca gcaacccgat
      481 ccgcgctttt cagctgcatt cggggcgtct ggttgtgccg caggcaaaaa gctgtatatc
      541 gggcggcact ggagtccgcc gtggccaaaa atacccctca aaaagcagcg atttcaccga
      601 cccgcgccga agactacgca cagtggtacc aagaggtggt tcgcgcggcc gacctcgccg
      661 agacctcacc ggtgcgcggc tgcatggtga tcaagccctg gggctacgcg atctgggaga
      721 acatccagcg cgttctggac ggcatgttca aggccaccgg gcacaagaac gcttactttc
      781 ctttgttcat cccgaagagc ttcctggaga aggaggccga gcacgtcgaa gggttcgcga
      841 aggagtgcgc ggtggtcacg caccatcgcc tagtcgctgg taaagacggc gggctgattc
      901 cggatccaga ggccgagctc gaggagcccc tcatcgttcg tcccacgtcc gagacgatca
      961 tcggcgcggc gttcgcgcag tgggtccaga gctaccgcga cctcccgcta ctcatcaacc
     1021 agtgggcgaa cgtggtgcgc tgggagcttc gcacgcggct cttcctgcgc actgccgagt
     1081 tcctctggca agaagggcac acggcgcacg caaccgaggc cgaggcgcgc gaagagaccc
     1141 tcaagatgct cgacgtctac gcgacgttcg cgcaggacta catggcgatg ccggtgatcc
     1201 gcggaccgaa aaccgcgagc gagcgcttcc ccggcgccgt ggacacgtac gccatcgaag
     1261 cgatgatgca agatcgcaaa gcgcttcagg ccgggacatc acacttcctc ggacagaact
     1321 tcgcgcaagc gagcggcatc aagttcctcg acgccgaagg gcagcagcgc ttcgcgttca
     1381 ccacgtcttg gggcgtctcg acccgcctcg tcggcggcct gatcatgacg cacgcggacg
     1441 acgatggcct cgtgctccct ccccgcctcg cacccgcgca cgcggtgatc tggccggtgt
     1501 tccgcggcga cgatacgcgc gcggcggtgc tcgagacgat cgagaagctt tctcagagcc
     1561 tgcgcgctgc gcgctacgat gatcgcccgc tccaggtcga gacggatctg cgcgacatcg
     1621 gcggcggcga caaacaatgg gagtggatca aaaagggcat cccgctccgc atcgagatcg
     1681 gcccacggga cgtcgcttcc ggcacggcca tggtggcgcg acgagatcgc ggccccaagg
     1741 agcgggagag cattccgctc gccgagctgc cgactcgcct ccccgcattg ctcgacgaga
     1801 tccagcggtc gctgtacgcg cgtgcgctca ttgtacgaga tcaggccaca agacccatcg
     1861 atgtacgctc ggagttcgac gccttcttca cgcccacgaa cgctcagaag ccggagatcc
     1921 acggcggctt tgcgctgtca cattggtgcg gcgaccccgc gtgcgaagcg accatcaaag
     1981 acgcgctgaa ggtcacgatt cgctgcattc caatgcacgg tatcgacgca tgcggcgccg
     2041 cgactcggct gaaggagccg ggcagctgca tcgtgtgtaa ccggccgagc gagcagcgcg
     2101 tggtattcgc gaagagctac tgacgggggc cgcctcccaa agcgcaacga gagatccgac
     2161 gaatcgcgtc aggtttctgg cgcgcacggg gccatctccg tccggcgaaa aaaatcccgg
     2221 tcggtggttg taatccgcct gagcggagtc gataacttag gctcagggct aagtgttgct
     2281 actcggttca atctcaagtc gaccgctgcg gatcttggtg gtggacgacg aggccacgaa
     2341 cctacgcgtg ctttcgagct acctgaagga cgcgaacctc gagccaaccg ccgtggggga
     2401 cggacgcgct gcgctcgacg tgttccccag cctggcgccc gacctcgtgt tgctcgattt
     2461 gatcatgccc gagctcggcg ggctcgacgt gttggtcggt attcggcaac accccgctct
     2521 cggtgagact ccggtcatcg tcatgactgg gttcaccgat cgacagctcc gcctcgaggc
     2581 catcgagcac ggcgccgacg acgtgctcga gaagccgctc gaccccgacg tgctgctcgc
     2641 gcgggtcgcg actctcatca agctgaagct ctcgcgggac gagctcaaag cgtcgcatgc
     2701 cgcgctcgct cggcaaaacg acgagctcga gcgggcgcag cgcgaacagc gcgagctcac
     2761 cgagttcatc gtccacgatt tgaagggccc gctcactggc atcgtcgcga acacggagtg
     2821 ggtctacgag cagctctcgc gccaagagag cggctacctc ggcgcgctcg aggacgtgct
     2881 ctcgagcgcg agtcggctcc gcgccatgat caacgacttg atgacggtct cgcagctcga
     2941 gcgcggcact ttcccggtcc gtcgacgcac cgtagccatg gggcccttgc tccgcagcgt
     3001 cgtgcgggac ttctcgcgcg ccgccgagca aaggcagatc gagctctccg cgccgcacga
     3061 gtgcgaagtc gatgtcgagg tcgacgtgga cttgattcag cgcgtgctcg ggaacatcct
     3121 cgagaactcg ctgcgctaca cgccggagcg cggcaaggtc gctctcgacg cgcacctcgc
     3181 tcagaagctc gagatcgaaa tcaagaacag cggaccgccg attccgaaat tcgagcgcga
     3241 cctcatcttc gagaagttcc ggcgcggctc cggcgcgggg ctcacgcacg gcaacgccgg
     3301 cctcggcctc tatttctgca agcgcgcggt gcaagcgcac ggcggagaca ttcaggtcgt
     3361 ggagactccg gactacccga cgtgctttct catccgacta ccggccgatt ccgtgaacga
     3421 cgcatcgctc gcctgaacga cgtcgccatt tcgactccac tccacagagt tatcgcgctc
     3481 actccgcgag cggcgccgcg cacgccgcgt catcggcgcg aggcttccgc ggtcgatgcg
     3541 cgatgccgac gcgcggccct cgcttttgta attcgctgaa atcgcgcgcc gcgagtgccc
     3601 gacgcgaaac ggtgggtttc ctttcgtcct cggtaagagt tggctccgat gccttggtcg
     3661 cgttggtttc ggggccgcag aaccccgaaa gcagcgaccg cacagctgct ttcttcatct
     3721 ggtggttggg gcgccccacc gaaaagggcg cgcaagacgg ggcgttcggg cgacgggcta
     3781 acaccaggac cagctgggct cgacggttcc gcgcacggcg cacgccgtac agcacgcgcc
     3841 gtcaccgagt cggggcttcc ttgcgtactc ggccggtgta ccatgtcggg ggatggtcct
     3901 ggtaagtccg gaaatcgacg cttttctggg ccgcgccatt ccctccgata gccaaccggg
     3961 cgtgcgcttc gagctcgaac gacgccttgg cgtcggcggc acggcggtgg ctttcctcgc
     4021 gtcgcgggtg agccccgagg ggcagtcacc ggccgtattg aaggtcatcc tgcccgccgt
     4081 cgcggcgagc cacggctcca ccgcagcgat ggtggtgaga aaagaagcgg tcgcgctcgg
     4141 ccggctcaac gagcgcgtgc cgcccacccc cttcgtggtg cggctcatgg acaccggggc
     4201 gttgccatac gtatttcgcg gcaagcacgt cgacctgcca tggctcgcga tcgagttcgt
     4261 cgacggcggc atcgagggaa cgaccctcga agagcgcatg gatcacacgc tgaacctcac
     4321 cggcaaggcc ttcgatcgcg agcgcgccgc gcgcgccatc tcgcacgtgt gcgcggggct
     4381 tgccgacgtg cacgcggtcg gtgtcattca ccgcgacctc acgcccaaca acgtgctctg
     4441 ttgcggcttc ggcgattcgg agctcttcaa gctctccgat ttcggtatcg ctcgccccgc
     4501 cggcgtcacg gacacgttcg ggcccagcgt catcggcacc ccgggctaca tcgcgcccga
     4561 gcacctctcg gactcggcga gcaccaccgc cggtgacatc tttgcgctcg ggtgcctcgt
     4621 ttacttcgtt ctcaccggcg accacctgtt cgacgtgacg aacgcgctcg agctcctcgc
     4681 gagcactcag caaaacaagc gtcgctcggt gcgagacgcc tccgcactca gcccggagct
     4741 tgccggagac gacgaagcgt gcgccaccat cgacgccatc atcgcccgcg cgaccgcgca
     4801 cgatccgctg cggcgacctg aaaatccccg tgaaatcgcg gccgcgctcg caccgctctt
     4861 actcgagcga aagggcagcg gctcacggcg cgagcgagcc acgctgatga gcgcagggca
     4921 ggcgctcggc atctctcggc tctcgtgcct cgtgcgtcac ccaccgggag acgaacgcat
     4981 cttgcgtagc gtcggctggg acggcgacgg ccactgcctc gccgcgtcga cgcgaggcct
     5041 cgaatactgg aacggctccg aatggcgagt gaccgagact cgcgacctgc ccggcgggca
     5101 agacgtgcgc ttcgttcgcc gtgtggggcc gggccgctgg ctgctcggcg gcgatcacgc
     5161 gctgctcgca gagtactcgc actcaggagt gagccgagtg atgcgcggac gtgacgccga
     5221 cgcgagcttt ctcgacgcga gcggtaacgt agccgatctc gccgcggtgg tggcgcaacg
     5281 cgcgacgggc acgatcgagc tctgcgcggc ggttgggggt cgctggctca ggccgttacc
     5341 gttgcccgac gccgcgttcg tgtccggact cacccaaatc gacgaggagc gctggctcgt
     5401 cgtcggacgc agccgacaag gcggcggctt cgtgctcgcg tactcgcctc tctcttggag
     5461 tgtcgagcca gtgctggcgc cgccgacccg cgcttgggtg gcatgcaccg cacgcacgga
     5521 aagagacgtg gtgttggcgg taggcaccga cggcaccgtc ttgcgcatcg agcggggaat
     5581 cgtcgacgga cgcgtcatcg ccgaacgacc gagcctcgcg agcgtgtccg tggacgtgct
     5641 cgatcgcgag tgggcgggcg gcgtcggcga gctatggtgc agcatcgcgg gcggcgattg
     5701 gacgcgggtt tggcacgacg ccacctggaa ccgcccgttc gtcagcctgt tcgccgacgt
     5761 ttcctcggtg gtagcgatga gcgtggacgg cggcgtgctc gagtgccggc ccgggctgcc
     5821 ttcgaccccg ccggggcgcg ctcgctgacc cggctcgcac tcgccgcgcc ccggttgctc
     5881 gagtgcgagc ggcgtgcgaa ggaggggcgt gcagtacttc gcatcttgcg ccaaaggcac
     5941 cgagggcgcc ctgcgggcgg agctcgccga gctcgggctc accggtgttc gcgctgaccg
     6001 cggcggggtt catttcggcg gcgagcgccc cgcggctttt cgcgcctgcc tttggtcgcg
     6061 catcgccgtg cgaatcctcg aaccgattgg gcagttcgag tgccccgacg aggacgcact
     6121 ctacggcggc gtgcacgggc tcgacttcac cgacgtactc tcgccgaagc acacgctctc
     6181 ggtgagcgcg gccgcttcga acagcaggct cacccacacg caatacatcg cgcaacgcac
     6241 gaaggacgca atcgtcgacc ggcaacgcga cgccttcggc gagcgctcca acgtcgagcg
     6301 ccgcgacccc gacgtgcacg tcttcgtgca cctctcgcgg gatcgggcga ccgtgtatct
     6361 cgacctcgcc ggaagtcccc tacacctccg cgggtaccgg cgcgcgcacg gcgatgctcc
     6421 gctgaaggag accttggccg cggcggtctt gcggctctct ggcttcgacc cgaggcgccc
     6481 ggtgctcgac cccatgtgcg gctccggtac tctgctcatc gaggcggcgc tctgggcgtc
     6541 gggacgggct ccgggcatct tccgtgagcg cttcggcttc gaacgctggg caagccatga
     6601 cgctcgcgca aagcaagtca tgaaagagtt gcgggaagcg gcgcgcggct cgagcgacgc
     6661 cctgcccgaa ttccggggaa gtgacgtcga cgctgcggtg ctcgacagcg cacgtgaaaa
     6721 cgccgagcga gcgggggtgc agctaacctt gtcgcatcgc tccatgatcg acgtccgccc
     6781 cgacgggacg aatggccttt tggtctcgaa cccgccgtat ggcgagcgac tcggagcgaa
     6841 cgccgatctg ccgcggcagc tcgcccgcct ggtcgatcgc tttccagacc actcggtcgg
     6901 tctactcatg ggcgccgaac agaacctcgc gcggactcgc agaaagccac gcatgttctc
     6961 gctgttcaac ggagacattg cgtgcaccct ccgcggctac gcaccgctcg atcgaagcgg
     7021 cacctcccgc gacagcgact gacctcgcgc cgctccacgg cgctgctccc ctctgcgtcg
     7081 taccgttcac cgcactcgaa ccaacgcacg cgccacctgg cgcatgtggt tccatgtggg
     7141 tccatccctc gcctgctcgt caacgccctc gagcgcgcgg cgcttgagca gcccattcgt
     7201 gagcggaacg gcgacgcgga gagctcggga tgcggcgctc tggcctcagc tccgagctcg
     7261 aatcgctact ttcgcgcgtg atgtcacagc ccctgcgcga ctgcgagcaa gcctttcacg
     7321 agcaccttcc gctgctgccc gcccatcact cgcgcccgta gcgccgcccc ggttcgcata
     7381 ctgcgcgagc cgcccgttca ccggatcgca atcccgcgat ggcacgacgc acttggccga
     7441 gcggtgccac ctgcgctcgc cctctacgct cgagcgacgc acgaagcccg atccggaggg
     7501 cgccttgtgt gtgggtccat cgcgacccgg accgcggcaa ataggatcta acgactgcga
     7561 catcgcgaac gtcgcggcga agtcgagcag cgatgactgc tcagtcgcgt gctttggcgc
     7621 cacgacattc acgcccaagc acttcggtcg ccgcgccaaa gcgcatcgac cgccgggttg
     7681 ggtatttcca tctggagcca ggtagtgcag cctccactcc gatgagcgcg gagtcaggcc
     7741 gcaaccagcg caacctggcg gaggcgaacg cggagagtcg cgaaacgtct cggccggcga
     7801 gcacgcgaca agtcgtgcgg cgacgcagta ttcgcgtcgc gcgcacgacg cgcgaccagc
     7861 gcgccttcgc gttgatgcga gcgcacgagc tcgaagagcg catcgaacgc ccgctcgtac
     7921 gccgtgattg tgaagacgca cctaggccct gccctttcgt ggcgtgccgc taccatctct
     7981 ttttggacgt ctcgccgagc accggcacaa tcaagttcaa cttccccgac ctagaggtct
     8041 gggagctcag cgagacgtgt gcgctcgacg ttgctgcgca aggtggccac ggacacgaac
     8101 gcacgagcga gctcatgaac gtgacgcgag agcgcgtccg gcagatcgaa gccgtggcgc
     8161 tcacgaaatt gaagcgagcg ggacggttgc gcgagctcga ggtcgatcgc tagccgactc
     8221 cccgttcgcc gtccgattcc gggcggccgc ctggattgct acgcccctcg atcgtcgaga
     8281 gcggttcggg gaagtaccgc gaggccgcgg aaggacagct caagaataga gcagcgcgct
     8341 cactaggcgc caaaccggag tgaacacgat cgggaacatc caccaagctc cgagcagccc
     8401 tgcgatttgc gcgacgggac tcatgcgaat gcgatcgtag aacgcgctga cttgcctggg
     8461 accgaaacct tgcacgaccg cagcaccgtc cagcggtggc accgggatca ggttgtagac
     8521 cccaagcagc acgttcagct tcagtaaagc gctcaacgcg agggcgagcg cgccgagcgg
     8581 cgacgaaaac tcctgtccgg gcgggagctg gacaaggccg acgctcgtgc cgtggtggcc
     8641 agacagatgc agcaccgacg ccgaatcgag cgctcgcagc gcgagcaacg caactagcgc
     8701 caacaggaag ttcgtcaggg gccccgcgaa cgacatgagc gcccagcggc gcgggtaacg
     8761 gacgccccaa tgcgggtcgt acggcgtgga cgcccagccg accatcatgc ccatctggaa
     8821 cgataaaatc ggcattatca ccatgccgaa cggctcccgc ttgatgtgcg gcagtggatc
     8881 gagcgtcacc tgccccatcg cgtaggcggt gttgtcgccg cccctcttgg ccgcgaaagc
     8941 atgcgccgct tcgtggcacg tcgtgctgaa tacgaagacc aagtaccaaa cgaggaacac
     9001 cgcgatcgtg ctcatccggg cacgcgcagc ctagccgaag ctcgccatct cgcccctcga
     9061 ggcgcaaaaa aagtccgttc tcggcctgga cgaaagccgg cccacagtct tggtaggcaa
     9121 aagccgcgaa aacctcgccg ccaaagaggc cgcggccctg tttggtggac ggcactgggc
     9181 cgatcctcgc gctggaccct ttgtcgtctc gaaggtccac gccctggttc attccgacca
     9241 cgtctgggcg gcgcgcgtgg cgctctcttg caactccacc cgcatcggct cggccccgag
     9301 gtcaacatcg atgcgccggt cagaccagaa ggttcgggcc aagttccgtt gagcctaaag
     9361 tcgaccgggg cgcgaacctg tcgctcgccc tgtcgctaaa cgaggcgcga gtggtggcca
     9421 ctggggtcgg gcggagagac cgcctatgcg ccaggagcag gccggttcgg gtcggccaag
     9481 cctcttgtcg gggccatcgt gctgcgtccg accgtcgggg ctcgcgctct ggggtaagcg
     9541 gccaagcgac cgacgtcaga ttcataatcg gaatattcag gccgattggc ctgcatcagt
     9601 cggtgctctc aaagtgactg ggtctgttgg ccgaatcttg gcaacaccac tgggaatcag
     9661 gcctattggc ctgcgttgct atttaggtcg gctgccaagc agattctgta ggtagccgcg
     9721 gtgaacgacc ggccttggtg agtagatgaa ggggctcgcg cgggccttca ccgaaacctg
     9781 caacgtgctc ggctctgcag gattatccgg cagcattatc gatgctgtat tctcaatttt
//
LOCUS       DASTKS010000873         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1927681, whole genome shotgun sequence.
ACCESSION   DASTKS010000873 DASTKS010000000
VERSION     DASTKS010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927681"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2732)
                     /locus_tag="VFQ35_18200"
     CDS             complement(<1..2732)
                     /locus_tag="VFQ35_18200"
                     /inference="COORDINATES: protein motif:HMM:NF037238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HET9932644.1"
                     /translation="MWGKVENHFQDLDRVDGAFRAENGKLYLFCDTQFIRYSAALEPG
                     NTAFYVDEGYPRRMSVWWAGEGTSLAMPNVWNALGSAVLRDAQATYAFDGATYKSSAS
                     PTPAPVIPTWARVRNEMQTQNRVDAGFVFGSGASATTLLFSADQYVRYSGAYSGFVDE
                     GYPKVIAHLAAADGAFPGIPSELLQGVRGFFSGTDGALHVFAPAPASDAAPQLYVSSA
                     SGDSLRPLNQRWGIIDNQLWDNEFVDAALRTTDGKLFLFSGDQYLRYSSADRSNVDEG
                     YPRKISTSYAAELGVASLAPEMNAGVDAALIVGTTHFYFVGSNVISSAAPTTAVSLVS
                     RWGLVRNFLQESGRLDAAFVAPNGKLHLFAGNQYAVYSGASRTYVDEEFPRVIAGDIG
                     QAWPSAFTQNLTAAAGFEGRSYLFQGSSHVRISDYRLRLVDAGYPIANSEKFLERFDF
                     ELGHLPLWWQAKQLFDDHSDTTPSLLEYVDTEVASDQTEALARATQWPSDTIAALLLI
                     YGLAEVDLLDLGTLSQLARALELAERVGSTPKKLKSEFWDRVFGASPAPVASLTQAAD
                     YLFQLIKATTSSQEFVTVARDLYNVVANAKRDALVAYLITHQIGNSALPAKLDANDVY
                     EYLLTDVQRDASLDTSYVVEAINSIQLFYHRALMGLEPSVPAELVDNLRLWWPWMKNY
                     RIWEANRKVFLHPENYIRPELRTEKSPAFEELEQKLLQDEITATSVRAAYQKYLEAFH
                     EIGRLRIVGGYRYEHLIELEGSSGGTTETATQIAVFMIGVSRTEPLVYYYRFGALQPP
                     RQDQDPDTTNIDWDPWQKVGITINAPRVQPVYAFNRLFLFWIESEPYNSTEFSSGDDK
                     TYSASSEGTKQVQLTVKYSFYNFTKEWAAPQSVRLNPGDATSPEALPHIFSASIA"
BASE COUNT          495 a          843 c          897 g          520 t
ORIGIN      
        1 gcgatgctcg cagagaagat gtgcggcaac gcttctggac tcgtcgcgtc gccggggttc
       61 agccgcacgg actggggcgc cgcccactcc ttcgtgaagt tgtagaacga gtacttgacc
      121 gtcagctgga cttgcttggt cccctccgag ctcgcgctgt aagtcttgtc gtcgccgctc
      181 gaaaactcgg tcgagttgta cggctcgctc tcgatccaga acaggaacag ccggttgaac
      241 gcgtacacgg gctgcacgcg cggggcattg atcgtgatac cgaccttctg ccaaggatcc
      301 cagtcgatat tggtggtgtc gggatcttga tcctgcctcg gaggttgaag cgctccgaag
      361 cggtagtagt agacgagcgg ctccgttcga gacacaccga tcatgaacac cgcgatctgc
      421 gtcgcggtct cggtcgtccc gcccgacgag ccctcgagct cgatgagatg ttcgtaacga
      481 tagccgccga cgatgcggag ccgaccgatc tcatggaacg cttcgaggta cttctggtac
      541 gccgcgcgga cgctcgtcgc cgtgatctcg tcttgaagta gcttctgctc gagctcctcg
      601 aacgcggggc tcttttcggt gcggagctcg ggacgaatgt agttctccgg gtggaggaag
      661 acctttcgat tcgcctccca aattcgatag ttcttcatcc acggccacca gagccgcagg
      721 ttgtcgacga gctccgcggg caccgacggc tcgaggccca tcagtgcgcg gtggtagaag
      781 agctgaatgc tgttgatggc ctcgacgacg tacgaggtat cgagcgacgc gtctctttga
      841 acgtcggtca gcaggtactc gtagacgtcg ttcgcgtcga gcttcgccgg cagcgccgag
      901 ttgccgattt gatgggtgat gaggtacgcg acgagcgcgt cgcgcttcgc gttggccacg
      961 acgttgtaga ggtcgcgcgc aactgtcacg aactcctggc tgctcgtggt cgccttgatg
     1021 agctggaaca gatagtcagc cgcttgcgtg agcgaggcga ccggagctgg agatgcgccg
     1081 aatacacgat cccagaactc ggacttgagc ttcttcggag tgcttccgac gcgctcggcg
     1141 agctcgaggg cgcgcgcgag ctgcgacaag gtgcccaggt cgagcaagtc gacttcggcg
     1201 agaccgtaaa tcagaagcaa cgcggcgatg gtgtcgctcg gccattgggt agctctggcc
     1261 aacgcctccg tttggtcgct cgcgacttcc gtgtccacgt actcgaggag cgacggagtc
     1321 gtgtccgagt ggtcgtcgaa cagctgcttc gcctgccacc agagcggcaa gtggccgagc
     1381 tcgaagtcga agcgctcgag gaacttttcg ctgttcgcga ttggataacc cgcatcgacg
     1441 agccgcaagc gatagtcgga gatacggacg tgcgaactcc cttgaaagag gtagctgcgg
     1501 ccctcgaacc ccgccgcggc ggtcaggttt tgcgtgaaag cagaaggcca cgcctggccg
     1561 atgtcacccg cgatgacgcg cgggaactcc tcgtcgacgt acgtgcggct cgcgccggaa
     1621 tagacggcgt actgattgcc tgcgaagagg tgaagcttcc cgttgggggc cacgaaggcg
     1681 gcgtcgagtc gcccgctctc ttgaagaaag ttgcgcacga gcccccaacg actgaccaac
     1741 gagacagccg tggtcggtgc cgcgctcgag atgacgttgc tgccgacgaa atagaaatgc
     1801 gtggttccga caatcaacgc ggcgtcgact ccggcgttca tctccggcgc gagcgacgcg
     1861 accccgagct cagcggcgta cgaggtcgaa atcttgcgtg ggtagccctc gtccacgttg
     1921 ctgcgatcgg cgctcgaata gcgcaagtac tggtcgccgc tgaagaggaa cagcttcccg
     1981 tcggtcgtgc ggagggcggc gtcgacgaac tcgttgtccc aaagttggtt gtcgatgatt
     2041 ccccaacgtt ggttcagcgg gcgcaagctg tcgccgctcg ccgaagacac gtagagctga
     2101 ggcgccgcgt cgctcgcggg cgccggcgcg aacacgtgca gagcgccgtc ggttcccgag
     2161 aagaagccgc gcaccccttg taggagctcg ctcgggattc cagggaaggc tccgtcggcc
     2221 gccgccagat gcgcgatcac ctttgggtag ccttcgtcga cgaaaccgct gtacgcgccg
     2281 ctgtaccgca cgtactggtc cgcgctgaac aggagggtcg tcgcgctcgc gccgctgccg
     2341 aacacgaagc ccgcatcgac gcggttttgc gtctgcattt cgttgcgcac tcgcgcccac
     2401 gttggaatga cgggggcggg agtcggagac gcactgctct tgtacgtcgc cccgtcgaaa
     2461 gcgtaggtcg cctgcgcgtc gcgcagcacc gcggaaccga gcgcgttcca gacgttcggc
     2521 atcgcgaggc tcgtgccttc gcccgcccac caaaccgaca tccgacgcgg atagccctcg
     2581 tcgacgtaga acgcggtgtt acctggctcg agcgccgcgc tgtagcgaat gaactgcgtg
     2641 tcgcaaaaga ggtaaagctt gccgttttcc gcgcggaatg cgccgtcgac gcggtcgaga
     2701 tcttggaagt gattttcgac cttgccccac accgagctca acagcttgtc cgacc
//
LOCUS       DASTKS010000874         6622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_140971, whole genome shotgun sequence.
ACCESSION   DASTKS010000874 DASTKS010000000
VERSION     DASTKS010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140971"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="VFQ35_18205"
     CDS             complement(<1..1694)
                     /locus_tag="VFQ35_18205"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932645.1"
                     /translation="MSETKRARVSPARSFDGFVDVRGARTHNLKNVHVKIPRDCLVVF
                     TGVSGSGKSSLAFGTLYAEAQRRYLESVSPYARRLFDQMEVPEVDVIEGLPPAVALQQ
                     QRGAPTTRSSVGSVTTLSNLLRMLYSRAGDYPANQPLLYAESFSPNTAEGACPRCHGL
                     GRIYDATEASMVPDDSLTIRERAVAAWPTAWQGQNLRDILTTLGHDVDVPWRELPKKT
                     REWILFTDEQPTVPVYAGYDMKEVRRALKRKEEPSYQGTFTSARRYVLETFATTESAM
                     MKRRVAAYLVSTECPECAGRRLRRESLSVKVRGRDITEISRLPLKELRGLFESLCDAS
                     SAEFAALERSHPEKALVLRRISEDLLARLEVLLDLGLGYLTLERSTPTLSPGELQRLR
                     LGTQVRSNLFGVVYVLDEPSAGLHPSDTEALLRALDGLKASGNSLFVVEHEVDVIRHA
                     DWIVDVGPRAGEHGGEVLFSGPPSELHQVRESETRRHLFAESQFEARSPRKPTGWLRL
                     SGVTRNNLTDLEVAFPLGVLTSVTGVSGSGKSTLVSQALVELVSRQLGERLVREEDV"
     gene            complement(1843..2472)
                     /locus_tag="VFQ35_18210"
     CDS             complement(1843..2472)
                     /locus_tag="VFQ35_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HET9932646.1"
                     /translation="MAAPRYPFLVPGVVAGSLVPFVSLAVRASRHLLGANPVSELLNQ
                     LGLLALVFLCASLACTPLKLALGWTWPMRVRKTLGLFAFFTALAHFSVYFALDQSFRV
                     GAVLTDIAKRPFILLGALALLLLVPLALTSTAAAVRRLGFKAWKRIHRLVYAAVVLAL
                     LHFYLRMKADHSLPIGYALLVGGMFAIRIYAALLKFRADARRAERTLQG"
     gene            complement(2472..3467)
                     /gene="msrP"
                     /locus_tag="VFQ35_18215"
     CDS             complement(2472..3467)
                     /gene="msrP"
                     /locus_tag="VFQ35_18215"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HET9932647.1"
                     /translation="MGNLPPGPKAAEITPQSLYQRRREFIKQGALFVGTSAVVGKSLV
                     TLSSMGGADSPATPPPVPSGLAGGAWQIRERGRYVVNESPTPYRDVTTYNNYYELGVS
                     KSEPAKLGFRIRPRPWTVRVSGELAEPRTLDVDTLIRWFGLEERVYRMRCVEAWSMVI
                     PWVGFPLAPLIHKLAPTSRAKFVEFTTLLDPEQLPNQDSGVLPWPYVEGLRIDEALHP
                     LTFLAVGLYGSPLPGQNGAPLRLVVPWKYGFKGIKSIVSIRFVESQPKSTWNVVAPHE
                     YGFYANVNPIVDHPRWSQATERRIGELERRPTLPFNGYAAEVAGLYSGMDLARYF"
     gene            complement(3515..3775)
                     /locus_tag="VFQ35_18220"
     CDS             complement(3515..3775)
                     /locus_tag="VFQ35_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HET9932648.1"
                     /translation="MSHHPTNFQGNVIEAITSAIQSQLPDAHAVVSGGGGHYTIEVVS
                     QAFAGKSTLESHRLVYSAIAHLMNGDAAPVHAVDSLKTRLPS"
     gene            complement(3788..4708)
                     /gene="grxD"
                     /locus_tag="VFQ35_18225"
     CDS             complement(3788..4708)
                     /gene="grxD"
                     /locus_tag="VFQ35_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET9932649.1"
                     /translation="MALSEALRQKFSDLVSNHRVVLFMKGTRRAPQCGFSAQVVQILD
                     ELAPDYRDVDVLESPEVRDGIKLFTNWPTIPQVFIDGKFVGGCDIVREMYQSNELQRL
                     LGVDVAPVLAPSVTLSAGALSALSAALEGSEGDVLHLEVSPRFDYDLFVAARRDSDLE
                     AKTEGPSVFFDSASARRVSSVSIDFIAGPEGGFKITSPEEPPKVREVDVNELKARLDR
                     GEIELFDVRPSHERALASIAQARPLDAEGQEFLRNLDRETPIAFHCHHGFRSQAAAQQ
                     MLAEGFRNVYNVTGGIEAWSQRIDPSVPRY"
     gene            complement(4831..>6622)
                     /locus_tag="VFQ35_18230"
     CDS             complement(4831..>6622)
                     /locus_tag="VFQ35_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9932650.1"
                     /translation="TNSEKIAVIRDPETGFDANDSFSPGKSYALVNAADGKQSQVAAP
                     VAWNSGALDASSGDHAWRFDFSKATAPGDYYVLDVEKNVRSYTFRIADDVYRDVLKQA
                     VRMFFYQRVGQEKTAAFAGAGWVDAGSHVGALQDHHARLYSDKGNAATEKDLWGGWYD
                     AGDYNKYTSWTAGYVENLLRCYSENPQAFSDDYGIPESGNGVPDVVDEAKWGMDYLIR
                     LQNADGSLVSIVGEASASPPSAATGQTLYGSPNTSGTLAGAAAFAYGARVFRALGNAT
                     YADDLLARAKKAYTWAEQNPSVVFKNNDDASGTSGLGAGQQEVDDYGRNMLKLDAAIQ
                     LFWATGDATYKTYVDANYTKAHLFAYTNYVAPWDVAAQDALLEYTKATGATANVVKAI
                     RDTYLAGASSSGNLGAITSDKDPYLSYMKDYVWGSNSTKSNTGLSLYDVVTFGLDSAA
                     AAEYERAAQRYVHYIHGVNPLSLVYLTNMGAHGAENSVNEMYHSWFSDGSAKWDRVGT
                     SMFGPPPGYLVGGPNPSYDWDQCCPSDCGAPSNNALCGSAPLSPPKGQPPQKSYKDFN
                     SNWPLDSWSVTEPSDGYQIAYIRLLSKFVK"
BASE COUNT         1211 a         2128 c         2117 g         1166 t
ORIGIN      
        1 acgtcttctt cgcgcaccag gcgttctccg agctgccgcg acacgagctc gacgagcgcc
       61 tggctgacga gcgtcgattt ccccgacccg gagacgccgg tcacgctcgt cagcacgccg
      121 agcggaaaag cgacctccaa atcggtcaga ttgttccgag tgacgccgct aagacgaagc
      181 caacccgtgg gtttgcgcgg cgagcgtgcc tcgaattggc tttcggcgaa cagatgacgg
      241 cgagtctccg attctctgac ctgatgcagc tcgctgggag ggccactgaa cagcacctct
      301 ccaccgtgct caccagcgcg cggtccgacg tccacaatcc aatctgcgtg ccgaatgacg
      361 tcgacctcgt gctcgactac gaacagagag ttgcccgagg ctttcagccc gtccagcgcg
      421 cgcaacaacg cttcggtatc ggatgggtgc aggcccgccg acggctcgtc gagcacgtag
      481 accacgccga acaaattcga acgcacctga gttccgaggc gcaagcgttg aagctccccg
      541 ggggaaaggg tcggggtgct gcgttcgagc gtgaggtagc cgagccccaa gtcgagcaag
      601 acctctaggc gcgctagaag atcttcggag atgcggcgca gcaccagcgc cttttcgggg
      661 tgagagcgct cgagcgcggc gaattcggct gaactcgcgt cgcagaggct ttcaaagagg
      721 ccgcgcaact ctttcaacgg cagccgcgaa atctccgtga tgtcgcggcc gcgcaccttc
      781 accgagagcg actcgcgccg cagtcgtcgc ccggcgcatt ccgggcattc ggtgctcacc
      841 aggtacgccg cgacgcggcg cttcatcatg gcgctctcgg tcgtcgcgaa cgtctcgagg
      901 acgtagcgac gcgcgctggt gaacgtaccc tgataactcg gctcttcttt cctcttgagg
      961 gcgcgccgca cctctttcat gtcgtagccg gcatagacgg gcaccgtcgg ctgctcgtcg
     1021 gtgaacaaga tccactcgcg cgtcttcttg ggcaattcgc gccaagggac gtcgacgtcg
     1081 tgcccgagcg tcgtcaagat gtcgcgcagg ttctgcccct gccaagcagt tggccacgcc
     1141 gcgaccgcgc gttcgcgaat ggtgagcgaa tcatccggca ccatcgacgc ttcggtggcg
     1201 tcgtaaatgc gcccgagccc atggcaacga ggacaggcgc cctcggccgt attgggcgaa
     1261 aatgactctg catacagaag tggctggttc gctgggtaat ccccggcccg cgagtacagc
     1321 atccgcaaaa ggttcgaaag cgtcgtcacg ctgccgacgg aagagcgcgt cgtgggcgct
     1381 cctcgttgtt gctgcagcgc aaccgcaggc ggcaagcctt cgataacgtc gacttccggc
     1441 acctccatct gatcgaaaag gcgccgtgcg tacggcgaca ccgactccag gtagcggcgc
     1501 tgcgcctcgg cgtagagcgt cccgaaagcg agcgaggatt ttcctgagcc cgatactcca
     1561 gtgaaaacca ccaggcagtc ccgtgggatt ttcacgtgaa cgttcttcag gttgtgcgta
     1621 cgggcgccgc gcacgtccac gaagccatcg aatgaacgag ccgggctgac gcgagcacgc
     1681 ttggtttcac tcatgcgggg aggcattggg gcagtcgtca atcggcggtc gcggctccgt
     1741 cagcccgact tccaagtgag gaatgtgtgt attcgaagcc acggacgcaa ctggggcaaa
     1801 aacgagcgca gtgtggccaa acgcgccatg acgacgcggc gctcaaccct gcagagtccg
     1861 ctccgcgcgt cgcgcgtccg ccctgaattt gaggagggcg gcgtaaatgc gaatcgcgaa
     1921 cataccgccg accagcagcg cataaccaat ggggagagag tggtccgcct tcatgcgcaa
     1981 atagaagtgc aagagagcca gcaccaccgc cgcataaacg aggcggtgaa ttcgcttcca
     2041 cgctttgaaa cccaggcgcc gaaccgccgc cgccgtcgag gtcaacgcga gcgggacgag
     2101 caacagcaac gcgagcgctc cgagcaggat gaacggacgc ttcgcgatgt ccgtcaaaac
     2161 cgcgcccacg cgaaagcttt ggtcgagcgc gaagtagacg ctgaagtgcg cgagggccgt
     2221 gaagaatgcg aacaggccga gcgtctttcg aacgcgcatc ggccaggtcc aacccaaagc
     2281 gagcttcagg ggcgtgcacg caaggctcgc gcacagaaac acgagcgcca aaaggccgag
     2341 ttggttcagg agctcggaaa ccgggttcgc acccagaagg tgccggctag cgcgtacggc
     2401 gagcgagacg aatggtacga gcgaaccggc gacgacgccg ggaaccagga acggataccg
     2461 cggagccgcc atcagaagta ccgagcgaga tccattcccg agtacagccc ggcgacctct
     2521 gctgcgtagc cgttgaaggg cagcgtgggc cggcgctcga gctcgccgat gcggcgttcg
     2581 gtcgcttggc tccagcgcgg atgatcgacg atggggttca cattcgcgta aaagccgtat
     2641 tcgtgtgggg cgacgacgtt ccaggtgctc ttcggctgcg actcgacgaa gcgtatcgat
     2701 acgatcgact tgatgccctt gaagccgtac ttccacggga cgacgagccg cagaggcgcg
     2761 ccgttttgtc cgggcaaagg cgacccatag aggccgaccg cgagaaacgt gagcgggtga
     2821 agcgcttcgt cgatgcgcaa gccctcgacg tagggccagg gcaagactcc gctgtcctgg
     2881 ttcggcagct gctcgggatc gaggagagtg gtgaactcga cgaacttggc gcgcgacgtc
     2941 ggcgcgagct tgtgaatcaa cggcgcgaga gggaagccaa cccagggaat gaccatggac
     3001 caggcctcga cgcagcgcat tcgatagact cgctcttcga gcccgaacca gcgaatcagc
     3061 gtatctacgt cgagcgttcg cggctcggcg agctcaccgc tgacgcgcac cgtccagggc
     3121 cgtggacgga tacgaaagcc gagcttcgcc ggctctgact tcgaaacgcc gagctcgtag
     3181 tagttgttgt acgtcgtgac gtcccggtac ggcgtcggcg attcgttcac gacgtagcgg
     3241 ccgcgttctc ggatttgcca tgcgccgccc gcgagcccgc tgggaacggg cggcggcgtt
     3301 gccggcgaat cggcgccgcc catactcgaa agcgtgacga ggctcttgcc caccaccgcg
     3361 ctcgtgccga cgaacagcgc cccctgtttg atgaactcgc gccgccgctg ataaagcgat
     3421 tgcggcgtga tttctgcggc tttcggcccc ggtgggagat tccccatgag cggactcctt
     3481 cgaaactctt gagccgctcg gcggaaagcg caagctaaga cggcaaacgc gttttcaggc
     3541 tgtccaccgc gtgcaccggc gcggcatccc cgttcatgag atgagcgatg gcgctgtaca
     3601 cgagccggtg gctctcgagc gtgctcttgc cggcaaacgc ctgcgaaacg acctcgatcg
     3661 tgtagtgacc gccgccgccg ctcacgacgg cgtgtgcgtc gggcagctgc gactggatcg
     3721 cgctcgtgat ggcttcgatg acgttacctt gaaaattcgt ggggtggtgc gacatgctct
     3781 tctcgaatca gtaacggggc acgctggggt cgatgcgctg cgaccacgct tcgatgccgc
     3841 cagtcacgtt gtatacgttg cgaaagccct cggcgagcat ttgctgcgcc gccgcctggc
     3901 tgcgaaagcc gtgatgacaa tggaacgcga tgggcgtctc gcgatccaag ttacgcaaga
     3961 actcctgccc ctccgcgtcg agcggccgcg cttgggcaat gctggcgagc gcgcgctcgt
     4021 gcgacggacg cacgtcgaag agctcgattt caccgcgatc gagccgcgcc ttcagctcgt
     4081 tcacgtcgac ttcccgcacc ttcgggggct cctcggggct cgtgatcttg aagccgccct
     4141 ccggacccgc gatgaagtcg atgctgaccg acgagacccg acgcgcgctc gcggagtcga
     4201 agaagacgct cggcccctct gtctttgctt cgagatccga gtcccgccgg gccgcgacga
     4261 acaagtcgta atcgaagcgc gggctcacct cgaggtgcag tacgtcgcct tcgctccctt
     4321 cgagcgccgc gctcaacgcc gacagagcgc cggcgctcag cgtcacgctc ggcgcgagca
     4381 ctggcgcgac gtcgacgccg agcaggcgct ggagctcgtt cgattggtac atttcgcgca
     4441 cgatgtcgca gccgcccacg aacttaccgt cgatgaagac ctgcggaatc gtcggccaat
     4501 tggtgaagag cttgatgccg tcgcgcacct cgggcgactc gagcacgtcg acgtcccggt
     4561 agtccggggc gagctcgtcc agaatctgca cgacctgcgc cgagaagcca cactgcgggg
     4621 cgcggcgcgt gcccttcatg aacagcacca cgcggtggtt gctgacgaga tccgaaaact
     4681 tttgtcgaag agcctcgctg agagccatgc aaagtccgat ctgccggccc agttgggcgc
     4741 ggggcgcctg tcttagcgcc gctcggaccg cttggcgact gctcccctcg cgaacagagc
     4801 cgtaccgcgc gcgatcgcgg ccgctccgac ctatttcacg aatttcgaga gcaggcgaat
     4861 gtacgcgatt tggtagccat cgctcggctc ggtgacgctc cacgaatcga gcggccagtt
     4921 ggagttgaag tccttgtacg atttctgggg cggctggcct ttggggggcg acaagggcgc
     4981 gctcccgcag agggcgttgt tgctgggcgc accgcaatcg ctcgggcagc actggtccca
     5041 gtcgtagctc gggttcgggc cacccaccaa atagcctggc ggcggaccga acatcgacgt
     5101 cccgacccga tcccacttgg cgctgccgtc cgagaaccac gagtgataca tctcgttcac
     5161 cgagttctcc gcgccgtgtg cgcccatgtt ggtgaggtac acgagcgaga gcgggttcac
     5221 tccgtggatg tagtgcacgt agcgctgggc cgcgcgctcg tactcggcgg ccgcggcgct
     5281 atcgagcccg aacgtaacca cgtcgtagag cgagagcccc gtgttcgact tcgtcgagtt
     5341 actgccccag acgtagtcct tcatgtacga gaggtacgga tccttgtcgc tggttatcgc
     5401 gccgaggttc ccgctgctgc tcgcgccggc gaggtacgta tcacggatcg ccttcaccac
     5461 gttggcggtc gcgccggtgg ctttggtgta ctcgagcagc gcgtcttgcg cggcgacgtc
     5521 ccagggcgcc acgtagttcg tgtacgcgaa taggtgagcc ttggtgtagt tggcatcgac
     5581 gtacgtcttg taggtagcgt cgcccgtcgc ccagaacagc tgtatggccg catcgagctt
     5641 gagcatgttg cgaccgtagt cgtcgacctc ttgctgtccg gcgccgagcc ccgacgtccc
     5701 gctcgcgtcg tcgttgttct tgaaaacgac gctcgggttc tgttccgccc aagtgtacgc
     5761 cttcttggct cgagccaaga gatcgtccgc gtacgtcgcg ttgcccaggg ctcggaacac
     5821 ccgagcgccg tacgcgaagg cagccgcgcc cgccaacgta cccgacgtat tcgggctgcc
     5881 gtacagcgtc tggcccgtcg ccgcggaagg cgggctcgcc gaagcctcgc cgacgatgct
     5941 cacgagcgag ccatccgcat tctgaaggcg aatcaggtaa tccatgcccc acttggcttc
     6001 gtcgaccacg tcgggcacgc cgttgcccga ctcgggaatg ccgtagtcgt cactgaatgc
     6061 ctgcggattc tccgagtagc atcgcagcag gttctcgacg taccccgccg tccagctcgt
     6121 gtacttgttg tagtcgccag cgtcgtacca gccgccccac aagtccttct ccgtcgcagc
     6181 gttgccttta tcgctgtaca ggcgcgcgtg gtgatcctgg agcgcaccca cgtggctccc
     6241 cgcgtcgacc cagcccgcgc ccgcgaacgc cgcggtcttc tcctgaccga cgcgctgata
     6301 gaagaacatg cgcacggctt gcttgagtac gtcgcgatac acgtcgtcgg cgattcggaa
     6361 cgtgtacgag cgcacgttct tctcgacgtc gagcacgtag tagtcgcccg gcgcggtggc
     6421 tttactgaaa tcgaagcgcc aagcgtgatc acccgacgac gcatcgagcg cgcccgagtt
     6481 ccacgcaacg ggagccgcga cctgcgattg tttgccgtcg gcagcattca cgagggcgta
     6541 gctctttccg ggagaaaagg agtcgttcgc gtcgaagccg gtctccggat cgcgaatcac
     6601 cgcgatcttt tcggagttgg tc
//
LOCUS       DASTKS010000875         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1703351, whole genome shotgun sequence.
ACCESSION   DASTKS010000875 DASTKS010000000
VERSION     DASTKS010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703351"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3626
                     /locus_tag="VFQ35_18235"
     CDS             <1..3626
                     /locus_tag="VFQ35_18235"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9932651.1"
                     /translation="FLTLILAFRPSESSGGGVERIAELRRLAEPCAPARIELESLDED
                     DINALCEHFASPCRDAERLSAFVCNGARGNPLFALEILKRLVQTGDLHWAREGTGGSW
                     RLLATRAERIPASANVVDFIIGRIDALPEPCRKVLTAASCIGHAFGFSELRVATELTA
                     PELMRAVDIVCAEGLVTPEPTGPQKSAEPIRGSSSSKRSVLRYSFFHDRIQQAAYSLC
                     SGDERQRIHLAVGRTRLRAVTGSGRSLFPAIEHLNRVHELLSPEEQRQLADHNFRAGV
                     AAKESVAYATAITLFRLALNALSDARGQRAQRLLFQCHLQLAECLYLNAEFAEAEAAF
                     LVALESARTLVDEVEVQRTRLVLYQHMQRYAEAISLGLATLKKLGVRMPEKPGTVGTL
                     SRLAPVMLRARRTPAEELFERPDHAEQTDRMILDLLVLLWTPSFWVNQSLNALVVLKL
                     IEVTLRLGNTPQAPMAYVCFGILHHVVFKQHERGLSFGRLAYRLVEDGADPFIASRVR
                     FLALTFFGPLERDNRENVAIYERALAKCLESGEHVFAGHTLDGISTSLPIHGFRIPEI
                     ERRLGVCDNVAERIGSDASRELVRVVRAFCRALVEHPNAPSASIDDRAIRYDSYLGVH
                     RLLRMVVCYLWDRDDDVRELAKALRGNQVVQSNPLHASFYSLFSVLAAARSPKGFQKR
                     VARRMLARLARFDSIQSQNFKSMLLLATAELQLREGHSDAALDSYHRAVSESAERGHD
                     LVHALSCERLAALFEARGDTASHHEYLAVAAYAYERFGATGKLRQLAQKYPNMRLERS
                     GAEVAASTLRATELDAEALMRAAYAIAEETRSDRLTENLLRVITTTAGGERGLLLKPN
                     DADWNVVAEWRTNQPRPDLLGGVLVPESVIRYVARTKGSVRLHGSELDPRFRSDPYFQ
                     DGNAPSALCLPLMHRGEVSAVLYLENGLCADVFTDAQQRLATMLGHQAAIAMAIADYH
                     KVQMDALQAKINPHFLYNALSVIAELVQKEPAKAESAVLKLSKLYRYVLSSSAEQVVT
                     LEQELGIVRDYLVLEQHRFGEKLKVEFSVTGATGNVRLPALIFQPLVENSVRHGIARK
                     LGPGCVKVTVSVGDSECCMRVEDDGPGWSGSGRKGGFGVRSVRERLALLYGGEGELTI
                     QKSPSVVAELRIPLDVVSRRAGPSSSARSGAAASERGASERSTDKGISSRDGERLQEV
                     GEA"
     gene            3623..3823
                     /locus_tag="VFQ35_18240"
     CDS             3623..3823
                     /locus_tag="VFQ35_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932652.1"
                     /translation="MTFAEALKLAEGWVDTVDGVEGVAEGLSGEEPCITVFVSSDAPG
                     GRLPAALGRWKIVLEGSTRRRA"
     gene            4070..5005
                     /locus_tag="VFQ35_18245"
     CDS             4070..5005
                     /locus_tag="VFQ35_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9932653.1"
                     /translation="MVDPSESFVQGMEVWVPSGGILRHHSGTYGQHAEFARVSANKSF
                     RYGEGLPGTVWASRRPEVWKELKSHFVRGEVAAAAGFDAAVGIPLFHGDEVSSVIVFL
                     CGRRERTGGCIEIWNTDAERRLLEHAAGYYGRFEQFGNISRLLQFQRGSGLPGMTWST
                     GLPQLVDVERRSSAFVRASVARDCGLDSGIAIPIHRAGSVAHVVILASAAATPIARAF
                     EIWLPYGQELVLEASQYGPGLEDFAESSRDTVFRKGEGLPGRAFASQLPVVFDHLRAP
                     QFTRYAAAARAGLEGGLAVPILDDLGVRAVVCLLN"
     gene            complement(5122..>5372)
                     /locus_tag="VFQ35_18250"
     CDS             complement(5122..>5372)
                     /locus_tag="VFQ35_18250"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HET9932654.1"
                     /translation="LKIESALVRSNSRAAGASPIKLRLRSETGALVVGVRRGDRLLEK
                     PDPTIPFETGDVVYFVGTSEAIARALPLFNPEPKSDLV"
BASE COUNT          858 a         1709 c         1837 g          968 t
ORIGIN      
        1 cgttcctcac tctcattctc gccttccggc cgtcggagtc gagcggaggg ggagtcgagc
       61 gcatcgccga gttgcgtcgg ctcgcggagc cgtgcgcacc ggcgcgcatc gagctcgaga
      121 gcctcgacga ggacgacatc aacgccttgt gcgagcactt tgcgtcaccg tgtcgagacg
      181 cggagcggct ctccgcgttc gtctgcaacg gcgcgcgcgg caatcccctc ttcgccctcg
      241 agattttgaa gcggctcgtg caaactggag atttgcactg ggctcgggag ggaaccggag
      301 gcagttggcg cctcctcgcg acgcgcgccg agcgcattcc ggcctcggcc aacgtggtcg
      361 atttcatcat cggtcgcatc gacgccctgc cagagccatg tcgaaaagtg ctgacggcgg
      421 catcgtgcat cgggcatgcc ttcggcttca gcgagcttcg cgtcgccacg gagctcacgg
      481 caccagagct catgcgcgcc gtcgacatcg tgtgcgccga agggctcgta acgcccgagc
      541 ccacgggccc acagaagagc gcggagccga ttagaggctc gagcagcagc aagcgctcgg
      601 tgctcagata ttcgttcttc cacgaccgca tccaacaagc ggcgtactcg ctgtgctcgg
      661 gtgacgagcg gcagcgcatc cacctcgcgg tcggccgtac tcggctgcgg gccgtgacgg
      721 gcagcggtcg ctcactgttc cctgcgatcg agcatttgaa ccgcgtgcac gagcttctga
      781 gcccagaaga gcagcggcag ctcgccgacc acaatttcag ggccggcgtg gccgcgaagg
      841 agtcggtcgc ctacgccacc gcgatcactc tgttccggct ggcgctgaac gcgctcagcg
      901 acgcacgcgg tcagcgcgcg cagcgcttgc tgttccagtg ccacctgcag ctcgccgagt
      961 gcttgtacct gaacgccgag ttcgccgagg ccgaggcggc gttcttggtg gcgctcgaga
     1021 gtgcccggac tctcgtcgac gaggtggagg tccagcggac gcgcctcgtg ctttaccagc
     1081 acatgcagcg ctacgccgag gcgatctcgc tcgggctcgc gaccttgaag aagctcggcg
     1141 tgcgcatgcc cgaaaagcca gggacggtcg gcacgctgag ccgtctcgcc cccgtgatgt
     1201 tgcgagcgcg ccggactcca gccgaggagc tgttcgaacg gcccgaccat gccgagcaga
     1261 cggaccggat gatcttggat ctcttggtcc tgctctggac tccgagcttt tgggtgaacc
     1321 agagcctgaa cgccctcgtc gttttgaagc tcatcgaggt caccctgcgg ctcggcaaca
     1381 ctccgcaggc accgatggcg tacgtgtgct tcgggatatt gcaccacgtc gttttcaaac
     1441 agcacgaacg tggcctctcg ttcgggcggc tcgcctatcg cctggtcgaa gacggagcgg
     1501 acccgttcat cgcgagccgc gtgcgctttt tggcgctcac gttcttcggc cccttggagc
     1561 gcgacaaccg cgaaaacgtg gccatttacg agcgggcgct cgcgaagtgc ctggagtccg
     1621 gcgagcacgt cttcgccggc cacacgctcg acgggatcag cacgtcgctg cccatccacg
     1681 ggtttcgcat tccagaaatc gagcgtcgcc tcggtgtgtg cgacaacgtc gccgaacgaa
     1741 tcggcagcga cgcgagtcgc gagctcgtgc gcgtcgtgcg tgcgttctgt cgcgcgctgg
     1801 tcgagcatcc caacgccccc tcggccagca tcgacgatcg cgccatccgc tacgattcct
     1861 acctcggcgt gcatcgcttg ctgcgcatgg tcgtttgcta tctctgggac cgcgacgacg
     1921 acgtgcgcga gctcgcgaag gcgctgcgag gcaatcaagt cgtgcagagc aaccccttgc
     1981 acgcgagctt ctattcactg ttcagcgtgc tcgcggccgc tcgctctccg aaaggctttc
     2041 agaagcgcgt cgcccgccgg atgctggcgc gccttgctcg cttcgactcg attcagagcc
     2101 aaaacttcaa gtcgatgttg ttgctcgcaa cggcagagct tcagctccgt gagggccatt
     2161 cagacgcggc gctcgactcc tatcaccgcg cggtgagcga gtccgccgag cgcgggcacg
     2221 atttggttca cgcgctgtcg tgtgagcgtc tcgcggcgct cttcgaagcg cggggtgaca
     2281 ccgcttcgca ccacgagtac ttggcggtgg ccgcttatgc gtacgagcgc ttcggcgcga
     2341 cgggcaagct gcgccagctc gcgcaaaagt acccgaacat gcggctcgaa cgcagcggcg
     2401 ccgaggtggc ggcttcgacc ttgcgcgcga cggagctcga tgcggaggcg ctcatgcgcg
     2461 cggcttacgc catcgccgag gagacgcgca gcgatcggct gacggagaac ctcctgcgcg
     2521 tcatcacgac gaccgcgggc ggcgagcgcg gtctcttgtt gaagccgaac gacgcggact
     2581 ggaacgtggt cgccgagtgg cgcacgaacc agccgcgtcc ggacttgctg ggcggtgtgc
     2641 tcgttcccga gagcgtcatc cgctacgtcg cgcgcaccaa gggaagcgtg cggctgcacg
     2701 gctccgagct cgatccgcgg ttccgctccg atccgtactt tcaggacggg aacgcgccgt
     2761 ccgctttgtg tctgccgctc atgcaccgcg gagaagtgtc cgccgtgctc tacctcgaga
     2821 acggcttgtg cgcggacgtg ttcaccgacg cgcagcagcg gctggcgacg atgctcggcc
     2881 atcaggcggc catcgcgatg gcgatcgccg actatcacaa ggtgcagatg gacgccttgc
     2941 aggcgaagat caaccctcac ttcctctaca acgcgctcag cgtcatcgcc gagctcgttc
     3001 agaaggagcc cgcaaaggcc gaatcggccg tgctgaagct ctcgaagctc tatcgctacg
     3061 tcttgagctc atcggcggag caagtcgtga cgctcgaaca agagctcggc atcgtgcgcg
     3121 actacttggt gctcgagcag caccgattcg gcgaaaagct caaggtcgag ttctcggtaa
     3181 ccggcgcgac cggtaacgtg cgtttaccgg cgctcatttt ccaacccctg gtggaaaaca
     3241 gcgtgcgcca cggcatcgcc cgcaagctcg gcccgggctg cgtgaaagtg acggtctcgg
     3301 tgggggattc ggagtgctgc atgcgggtcg aagacgacgg gcccggttgg agcggcagcg
     3361 gacgcaaggg cgggtttggg gtccgtagcg tccgcgaaag gctcgcgctc ttgtacggcg
     3421 gcgaaggcga gctcacgatt caaaagagcc cgtcggtggt cgcggagctt cggatcccgc
     3481 tcgacgtcgt gtctcgacgc gcggggccgt cgagctccgc tcggagcgga gcggcggcga
     3541 gtgagcgagg ggcgagcgag cgttctaccg acaaagggat ctcgtcgcgc gacggcgagc
     3601 gattgcagga ggttggggaa gcgtgacttt cgcggaagca ctcaagttgg cagagggttg
     3661 ggtggatacg gtggacggtg tcgagggggt ggcggagggc ttgagcggcg aagagccgtg
     3721 catcaccgtg ttcgtgtcct cggacgcgcc cgggggacgt ttgccggctg cgctcggccg
     3781 ttggaaaata gttttggagg gctcgacgcg gcgtcgcgcg tgaaaggcgc gaggcgacct
     3841 ggcggaatct gtcgcgcgtt ggcgccacgc gtttttgtcg acagttctcg atcgcgtgcg
     3901 gccaagccct ccgagcgctc ctgtttccaa ggtcgggaaa gcgccgctcg tcgcaatgcg
     3961 gaagaaacct agcggttgct cggggattgt agactcgcag agtcggtcat actcgctcgc
     4021 gcagcgtttc gcgtgcgccg cgcgaggcgc tttggcggaa acccagcaca tggtggatcc
     4081 aagtgaatcg ttcgtgcaag ggatggaggt atgggtgccg agcggcggca tcctgcgtca
     4141 ccactcgggg acatacgggc agcacgccga gttcgctcgt gtgagcgcga acaagtcctt
     4201 tcggtatggc gaggggctcc cgggcacagt atgggcttct cgacgtccgg aagtttggaa
     4261 ggagctcaag tcgcatttcg tgcgtgggga ggtcgccgcg gcggcaggct ttgacgccgc
     4321 cgtcggtatc ccgctgtttc acggtgacga ggtgagttcg gtgatcgttt tcttgtgcgg
     4381 ccggcgagaa cgaacgggcg gctgcatcga gatctggaac accgacgccg agcggagact
     4441 gctggagcac gcggctggct actacggccg cttcgagcag ttcgggaaca tcagccgact
     4501 cctgcagttt cagcgcggct ctggtttgcc tgggatgact tggtcgacgg gcttgcctca
     4561 gctcgtcgac gtcgagcgtc gctccagtgc tttcgtacgt gcatcggttg cgcgcgactg
     4621 cggcctcgac agcggtatcg ccatcccgat tcatcgcgcg ggttcggtag ctcacgtcgt
     4681 cattctggct tccgccgcgg cgacgcccat cgcgcgcgcg ttcgagatct ggctccccta
     4741 cgggcaagag ctggtgctcg aggcatcgca gtacgggccg gggctcgagg acttcgcaga
     4801 gagcagccgc gataccgtat ttcgaaaggg cgagggtctc ccgggccgag cctttgcttc
     4861 gcagcttccc gtcgtcttcg atcatttgcg cgccccgcag ttcacgcggt acgcggccgc
     4921 agcccgtgcg ggcctcgagg gcggtctcgc cgtgcccata ttggacgacc tcggcgtacg
     4981 ggcggtggtt tgcctgttga actgagaccc tgcgaatgga cgggtcagcc gcgagcgagc
     5041 cgaacctctc gcggcgtgcc gagcctctcg gtgcggaggc gcgcgacggc gacgcgctcc
     5101 cgccgatgcc tgctcgccgc gtcagacgag gtcgctcttc ggctccgggt tgaacagagg
     5161 gagagcgcgg gcgatcgctt cgctcgtgcc gacgaagtag acgacgtcgc cggtttcgaa
     5221 cgggatggtc ggatccggct tctcgaggag ccggtcgccc cggcgcaccc ccaccacgag
     5281 cgcgccggtc tcgctgcgca gccggagctt gatcggggaa gcgccggccg cacggctgtt
     5341 gctccgcacg agcgcggact cgattttgag ct
//
LOCUS       DASTKS010000876         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1257446, whole genome shotgun sequence.
ACCESSION   DASTKS010000876 DASTKS010000000
VERSION     DASTKS010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257446"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFQ35_18255"
     CDS             <1..377
                     /locus_tag="VFQ35_18255"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9932655.1"
                     /translation="RTVDVWATDENFWVRRTALLSFHDALLAGNGDFDHFERIATPML
                     VEREFFIRKAIGWVLRSTTRRAPELVIGFVERHAREMSTLTFHEATRRLPLREQRRLR
                     TLREDGELSRKPARKPKARRSS"
     gene            547..1584
                     /locus_tag="VFQ35_18260"
     CDS             547..1584
                     /locus_tag="VFQ35_18260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9932656.1"
                     /translation="MSKVISYVADMTRQRERHVLEASLADVLFKLLKPTSLRIWRVHA
                     QAAEARVQLVTHMEATPSAPEAMNEAAEWPLLDSLPGVRDCYRSGAPLWIDAESGHER
                     RHFLPVTSEKETIGVLEIERRSLTDAQLQIADGMLTIYRNHLALLDYSEHDELTGLLN
                     RKTFESVFSKRAGERRPDEIDPNLHIIGRRTLRQGAKPWLAVVDIDHFKRVNDTFGHV
                     FGDEVLLLLAGIMRSTFRTEDALFRFGGEEFAVLLAATDEPGAETALERFRRTVETHV
                     FPQVGSVTVSVGYCEVLESEDAMSTFERADAALYFAKQHGRNQVQRYEALVMKGKLVP
                     KAKPMRDIELF"
     gene            1924..>2352
                     /locus_tag="VFQ35_18265"
     CDS             1924..>2352
                     /locus_tag="VFQ35_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932657.1"
                     /translation="MTHAPVDVGVQVGGETPFGLHVYGGVGWIPPAYAGLLRGIAASA
                     TSDPDAHAVLEQASYSGNTWRVQAGIRPFKEVGLYLDGGYSHLTFSGSANLSESGAPN
                     LVAIGGNYVSTTKLDAWFVELGYLGEVKDRVVIGGALGLMR"
BASE COUNT          436 a          752 c          748 g          416 t
ORIGIN      
        1 agcgaacggt ggacgtttgg gcgaccgacg agaacttctg ggtgcggcgt acggcgctcc
       61 tctcgttcca tgacgcgctc ttggccggaa acggcgactt cgaccatttc gaacggattg
      121 cgacgccgat gctggtcgag cgcgagttct tcattcgaaa ggcgatcggt tgggtgctgc
      181 gttcgacgac ccgcagagcc cccgagctcg tcatcggctt cgtcgagcgg catgcgcgcg
      241 agatgtcgac tctcacgttt cacgaagcga cgcggcgcct gccactccgg gagcaacggc
      301 ggctgcgaac tctccgcgaa gacggcgagc tctcccgaaa gcctgcgcga aaaccaaaag
      361 cgaggcgcag ctcctgatac agagtgcgac acgagcccca cccgagcgtg acgacacaaa
      421 tcatctcgcc gcacgctccc gaactcgcct ggttcgaagc ttctgaattt tcgagcgcgg
      481 cggccgctcg tctcgaggaa ggctcgtccg aagcggcaac gccgacgaca gcgtccttgc
      541 tgctcaatgt ccaaggtgat ctcgtacgtc gccgacatga cgcgtcagcg tgagcgacac
      601 gtcctcgagg cttcgctggc agacgtcctc ttcaaattgt tgaagccgac atcgttgcgt
      661 atctggcgcg ttcacgcgca ggcggcggag gctcgcgtgc aactcgtcac ccacatggag
      721 gcgacgccca gcgcgcccga ggccatgaac gaagctgccg agtggccgct gctcgattcg
      781 ctgcccggcg tgcgcgactg ctatcgcagc ggagctccgc tttggattga cgccgagtcg
      841 gggcacgagc gccgtcactt tcttccggtc acgagcgaga aggagacgat cggcgtgctc
      901 gagatcgagc ggcgctcctt gaccgacgct cagctgcaga tcgccgacgg catgctgacc
      961 atctatcgca accacctcgc actgctcgac tacagcgagc acgacgagct caccgggctg
     1021 ctgaaccgca agactttcga gagcgtcttc tccaagcgcg ccggagagcg ccgccccgac
     1081 gaaatcgatc ccaacctgca catcattgga cggcgaacct tgcgccaggg cgcaaaaccc
     1141 tggctagccg tcgtcgacat cgaccacttc aagcgcgtga acgacacgtt cggtcacgtg
     1201 ttcggtgacg aagtgctgct gctgcttgca ggcatcatga ggtctacatt ccgcaccgaa
     1261 gacgctctct ttcgctttgg gggcgaagag ttcgcggtat tgctcgcggc gacggacgaa
     1321 cccggcgccg agacggcgct cgagcgcttc cgtcgcacag tcgagacgca cgtgttcccg
     1381 caagtcgggt cagttaccgt gagtgtgggc tattgcgagg tgctcgaaag tgaagacgcc
     1441 atgtcgacgt tcgagcgcgc ggacgcggcc ctgtattttg ccaagcagca cggccgcaat
     1501 caggtgcaaa gatacgaagc cctcgtaatg aagggcaaac tcgttccaaa agcaaagccg
     1561 atgcgtgaca tcgagctctt ctagccctag atccactagc ggcgcgcggc actcggccgg
     1621 cacgcgtcgg tggcgggcac ggaaggtgca tcggagggag tcatgatcgc gccgtttggc
     1681 tggaattcgc gcaaagccaa gggctcaacg ccccagcgga ccgcaaggtg gatcgcttgc
     1741 gccttggctt gcgtcgcccc gcaagcattt gcgcgtgaaa aaccgctcga agtccccgag
     1801 gaaagcccct cgagcgccga cgcgttcgtg gccgcctcga ggcgaactga atcgccgcgc
     1861 gatacatctc cgtcttccgc gcggagcaag ggtctccaag cgtggctgct gtcggtagaa
     1921 gcggtgacgc acgctccggt cgatgttggc gtgcaggttg gtggcgaaac gccgttcggg
     1981 ctccacgttt acggcggagt cggctggatt ccccccgcgt acgccgggct cttacggggc
     2041 atcgctgcct ccgcgacgag cgatcccgac gcgcacgccg tgctcgagca agcgagctac
     2101 agcggcaata cgtggcgcgt gcaggccggt atccgtccgt tcaaagaagt tggactttat
     2161 ctcgacggtg gctactcgca cctcacgttc tccgggagcg ccaatctctc ggagagcggt
     2221 gcgccgaacc tcgtcgcgat cggtgggaac tacgtctcga cgacgaagct cgacgcgtgg
     2281 ttcgtcgagc tcggctatct cggtgaagtg aaggatcgcg tcgtcatcgg cggcgcgctc
     2341 ggcctcatgc gt
//
LOCUS       DASTKS010000877         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_587047, whole genome shotgun sequence.
ACCESSION   DASTKS010000877 DASTKS010000000
VERSION     DASTKS010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587047"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFQ35_18270"
     CDS             <1..515
                     /locus_tag="VFQ35_18270"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HET9932658.1"
                     /translation="RYVVTEVRFLLNRIEPAKRRYPELAKSLAELPHPPTLSDVRNAV
                     LTLRRSKSMVLESDDPNSRSCGSFYLNPIVSRERADALERALSVETMPRFPQPDGNVK
                     LSAAWLIERSGLRKGQTHGNVGISTKHALALVCHDGASSAELLAFSRHVQEVVRSRTG
                     IELCPEPQFL"
     gene            527..1591
                     /locus_tag="VFQ35_18275"
     CDS             527..1591
                     /locus_tag="VFQ35_18275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9932659.1"
                     /translation="MVDDRFRNAFRFPGLDALRGISILAVLWHHATPRQLAGVLGRGH
                     LGVQLFFSISGFLITTLLLRERRETGDVDLSRFWVRRALRLFPLYYVTLAGFVAFAWA
                     LPTSLPERAHFFQSLPFYVTHTANWFVDGRVTHPMLFAFAWSLSTEQQFYLVWPSLIK
                     RLREPVVLGVVLVLLIVADQLLERRALGLHFSRSAFFVLTSFSASIALGALAALLLEV
                     GGSHRRFESWRPRAWLEVVCTALVAFGVLSPPRHFIWFEGSLALLVACAALAGDQGLW
                     ARLAKTACGELGRRSYGVYLFHVPVLGALRWVMPGLRAEPLPLFLLAVPVCWALAELS
                     HQHFEARFLARRPRLERQSI"
     gene            complement(1607..>2212)
                     /locus_tag="VFQ35_18280"
     CDS             complement(1607..>2212)
                     /locus_tag="VFQ35_18280"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET9932660.1"
                     /translation="GLFKIVSEQGVAAGVRRIVAVTGDGALAYLRELEGQLSKAAEVL
                     KTTPQQLPERLEKVLLRERALEKQIEELTRKLMSGGSSGFDSWVGQARDVSGVKVLGL
                     KSDVTDRAALRELAEKLRDKLGESVVLVGSVHDGKAQLVLTVSKPLTNRLRAGDLIRP
                     IAEIVGGSGGGRPDMAQAGGTDPGRLDEAIEAVYRGVGALN"
BASE COUNT          314 a          746 c          704 g          448 t
ORIGIN      
        1 ggcgctacgt ggtgaccgaa gtgcggtttc tattgaaccg catcgagcca gcgaaacgcc
       61 ggtacccaga gcttgccaag agcctcgcgg agctccccca ccccccaacc ctgagcgacg
      121 ttcgaaacgc cgtcctcact ctgcgtcgca gcaagtcgat ggtgctcgag tcggacgatc
      181 cgaattcgcg cagctgtggc tcgttttacc tgaacccgat cgtgtcgcgg gagcgcgcgg
      241 acgccctaga gcgcgcgctc tcagtcgaga cgatgccgcg atttccgcag ccggacggca
      301 acgtcaagct ctccgccgct tggctcatcg agcgctccgg ccttcgcaag gggcaaaccc
      361 acggcaacgt cgggatctcg acgaagcacg cgctcgcact cgtgtgccat gacggcgcga
      421 gcagcgcgga gcttctggcg ttttcgagac acgtgcagga agtcgttcga agtcgcaccg
      481 gcatcgagct ctgtcccgaa ccacagtttc tttgagcgcg agcgtcttgg tcgacgaccg
      541 attccgcaac gcttttcgct ttccggggct cgacgcgctc cggggcatat cgatcttggc
      601 ggtgttgtgg caccacgcga cgccccgtca gctcgcgggg gtgctcggcc gcggacacct
      661 cggagttcag ctgttcttct cgatcagtgg ctttctgatc acgactctgc tgttgcgcga
      721 gcgacgcgag accggcgacg tcgatttgag tcgcttctgg gtgcggcgcg cgctccggct
      781 ctttccgctc tactacgtta cgctcgcggg gttcgtcgcc ttcgcctggg ccttgcctac
      841 ctctctgccg gagcgggcac acttcttcca gagcctgccg ttttacgtga cgcacacggc
      901 gaactggttc gtggacggtc gagtgacgca cccgatgctg ttcgcgttcg cgtggtcgct
      961 ctcgaccgag cagcaattct atttggtctg gccgtcgctc atcaaacggc tgcgcgaacc
     1021 ggtcgtgctc ggggtggtac tcgtgctcct catcgtcgca gaccagttgc tcgagcgacg
     1081 cgcgctcggc ctacactttt cacgaagtgc gttcttcgtg ctcacgagct tcagtgcatc
     1141 gatcgcgctc ggtgcgctcg cggcgctctt gctcgaggtc ggtgggagcc atcgacggtt
     1201 cgagtcgtgg cgcccgcggg cgtggctcga agtcgtttgc acggcgttgg tggcgttcgg
     1261 ggtattgtcg cctccgcgcc atttcatttg gttcgagggc tcgctcgcgc tgctggtcgc
     1321 ttgcgcggct cttgcaggtg accagggctt gtgggcgcgg ctagcgaaga ctgcgtgcgg
     1381 cgaactcgga cgccgcagct acggcgtgta cctgttccac gtgcccgtgc tcggcgcgtt
     1441 gcgctgggtg atgccagggc ttcgtgccga gccgctgccg ctcttcttac tggccgtccc
     1501 agtgtgttgg gcgctcgccg agctcagtca tcaacatttc gaggcgcggt tcctcgcccg
     1561 gagaccacgc ctcgaacgtc aatcgatttg acggctcgct agcgactcag ttgagcgcgc
     1621 cgacgccgcg gtaaacggct tcgatcgcct cgtcgagccg gcccggatcg gtgccaccgg
     1681 cttgcgccat gtcggggcgt cctccgccag agccacccac gatttcggcg atcggccgga
     1741 tcaggtcgcc cgcgcgcagc cgattggtga gcggcttcga aacggtgagc acgagctgag
     1801 ccttgccgtc gtgcaccgaa ccgacgagca cgacgctctc gccgagcttg tcgcggagct
     1861 tttcggcaag ctcgcgcagc gcggcgcgat ccgtgacgtc gctcttcaag ccgagcactt
     1921 tgacgccgga gacgtcgcgc gcctgaccaa cccagctgtc gaaaccgctc gagccgccgc
     1981 tcatcagctt gcgggtgagc tcctcgattt gcttctcgag cgcgcgctcc ctcaagagca
     2041 ctttttcgag ccgctccggg agctgctgcg gagtggtctt cagcacctcc gccgccttgc
     2101 tgagctggcc ctcgagctcg cgcagataag cgagcgcgcc gtctcccgtg accgcgacga
     2161 tacgacgcac gcccgcggcg acgccctgct cgctcacgat cttgaagagc cc
//
LOCUS       DASTKS010000878         6227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1703778, whole genome shotgun sequence.
ACCESSION   DASTKS010000878 DASTKS010000000
VERSION     DASTKS010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703778"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFQ35_18285"
     CDS             <1..300
                     /locus_tag="VFQ35_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932661.1"
                     /translation="PHWTVFECTPSLHLLGQAPDAHPARLQGEALVRCVAALRPSYDF
                     IVIDGPLLDDAAACEAVREVVDSVVFSHGRYGPSEITRVKSLFPQKRISLVPAVG"
     gene            complement(344..3196)
                     /gene="uvrA"
                     /locus_tag="VFQ35_18290"
     CDS             complement(344..3196)
                     /gene="uvrA"
                     /locus_tag="VFQ35_18290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9932662.1"
                     /translation="MKSREPELVVVRGAREHNLKNVTVQIPKHQLVVLTGVSGSGKSS
                     LAFDTLYAEGQRRYVESLSSYARQFLGQMEKPKYDSIRGLSPTISIEQKSASSNPRST
                     VGTVTEVHDYLRVLYASIGVQHCPSCGKPARAQSAQQIVDSLLDEQKGQQVTILAPLE
                     RQRKGTHLERLAELKSRGFVRARIDGKLVKLDGTEPSLDKKKKHDLDLVIDRVTATTA
                     ERTRLTDSVESALREGQGSVIIAGSSAERTYSEKAACVDCGLSFPELAPLAFSFNSPL
                     GFCHECNGLGSRPEMDPDLVVPNAKLSIRQGAITVWAGAMSRGEGWKASGIEWIADTF
                     QIDLDKPWEKLPKSQRDAILFGGKRGRAEWEGLCNQMMRRLKATESEEMKQYYLRFFS
                     SKACPACKGARLRPESRAVLIHGQGIDGVSALRIEAALEWIKKLPLKGADAHIAEELQ
                     KEISARLGFLTNVGLGYLTLDRAAATLSGGESQRIRLASQIGSELTGVIYILDEPSIG
                     LHQRDNQRLLDTLKALRDLGNSVIVVEHDEETMREADHLIDFGPGAGVLGGEVVAAGT
                     PEQVMREPRSLTGGFLSGRLRIELPNERRPGNGKAIWVRGAREHNLQNLDVRFPLGTL
                     VCVTGVSGAGKSTLVNGILRPALSRALHDAREPVGEHRGIDGLEHIDKVIAIDQKPIG
                     RTPRSNPATYTKVFDSIRELFAGLPEARAYGYDAGRFSFNVKGGRCEACSGDGLRCVE
                     MHFLPDVYVTCEVCKGHRFNDATLRVRYKGKNIAETLELSVSEGLEQFSVHPQIMRGL
                     KTLDDVGLGYLKIGQPSPTLSGGEAQRIKLARELSRVGTGRTLYILDEPTTGLHFADV
                     ERLLGVLSRLVEAGNTLVVIEHNLDVIAHADWVIDLGPEGGSSGGRLVGQGTPEELAK
                     VRESYTGRYLAEALARARGAQSSARPAAVGGRRKRA"
     gene            complement(3210..4043)
                     /locus_tag="VFQ35_18295"
     CDS             complement(3210..4043)
                     /locus_tag="VFQ35_18295"
                     /inference="COORDINATES: protein motif:HMM:NF020990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET9932663.1"
                     /translation="MASYDSARDCYVVRVVYDGPGYAGKTTNLQRICDQVPTTKRSEM
                     YTPAALKGRTMFFDWVEVDGPKQNRRSLKFQLISVPGQIERNYRRRPLVEMADVVVFV
                     CDSSPRQVNDTLRTFARLRTSMRQRETPVPLVVQANKQDVSGAISPKEIRKRLDLEED
                     TPVVSASAADGRGVQETLRTAVRLGVKTMVDSGRVSLLPPEFANADALFDHVLTFEDA
                     PRDDGPVEVEELNVNAEYSSLEEEAMLAHLAASTSIDALESRARRAARRREEDSEVKE
                     R"
     gene            complement(4271..>6227)
                     /locus_tag="VFQ35_18300"
     CDS             complement(4271..>6227)
                     /locus_tag="VFQ35_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932664.1"
                     /translation="PSALLRVSELSAEHGAPERTFAVCDVLLDRHEAELSPTDLATTL
                     RRRGEALFKLGRVEAAVDSLERAIRADAASPQSFRALGKVYAAREEWERVIETRYREL
                     ELVEGDERVQTLIEIGEVAAGKLHNTDYAARALLLALDERPNDRNILARLMQLYSAEK
                     DWPQLIEVIMRLAEVVEDDKQKGKYLYAAAMVAARELREPARALVYIDLALDADPDNE
                     SAFSEGVKLRRQMQDFEGLKDMFKRRAQDLAAREDKGNLMEILEELGDIYDERLARRD
                     QAIRVFESALELEKDNARISERLALLYSAEPAAYFDQALQALGAWIKRDPYQPQPYKM
                     LRKVYTEARRADGAFLACQALHVLGQAEPDETRFFSRMRDSEAPQIARGLSAQEWMEL
                     IAPETADPLLTALFALVEPYVIQARAQHPEVLGLGEEHRIDIGNYPYGLVLATHNAAE
                     ALGVPVPTMFQKPEDPGVLGFLPTAPPSVLVGGGAFNGNFDSLQTAFIAGHHVAYYQP
                     GLYLRQLLPNLTALKAWLFAAIRLVKPKFPATADLEAPITDASKVLQRLATGAGIEQL
                     THIVTKLLASETALDLKRWVHAVDFSADRAGLAVAHDLETAGALVQSVPAAQGSPSVE
                     ARLENLMGYAVSEQYLELRARQGVAID"
BASE COUNT          979 a         2109 c         2029 g         1110 t
ORIGIN      
        1 ccgcactgga ccgtgttcga gtgcacgccg tcgctccact tgctcgggca agcgcccgat
       61 gcacaccctg ctcgtctcca aggcgaagcg ctcgtcaggt gcgtggcggc gctgcgcccc
      121 tcttacgact tcatcgtcat cgacggaccg ctgctcgacg acgccgccgc ttgcgaagcg
      181 gtgcgcgagg tcgtcgacag cgtcgtgttt tcccacggtc gctatgggcc gagcgaaatc
      241 acgcgggtga aatcgctctt cccgcagaag cgcatctcgc tcgtccccgc ggtgggctga
      301 gccgcttctg attcgttcgg cgcgttctcg agcttcgtcg cgactaggcg cgctttcgcc
      361 ggcccccgac cgcagccggc cgcgcgctgc tctgcgcccc tcgagcgcgc gccaacgcct
      421 cggcgaggta acgacccgta tagctctcgc gcactttggc gagctcttcc ggcgtgcctt
      481 gcccgacgag gcgaccgccg ctcgagccac cttcgggccc cagatcgatg acccagtcgg
      541 cgtgcgcgat gacgtcgagg ttgtgctcga tgacgaccag cgtgttgccg gcttcgacca
      601 agcgcgaaag gacgccgagc aagcgctcga cgtcggcgaa gtggagcccc gtcgtgggct
      661 cgtccaaaat gtacagggtg cggccggtac cgacgcgcga aagctcgcgc gcgagcttga
      721 tgcgctgagc ttcgcctccc gaaagcgtcg gcgagggctg gccgatcttc aagtaaccga
      781 gccccacatc gtcgagcgtc ttcaagcctc gcatgatttg cgggtgcacc gagaactgct
      841 cgaggccttc gctcacgctg agctcgagcg tctcggcgat gttcttgccc ttgtagcgca
      901 cgcgcagggt cgcatcgttg aagcggtgcc ctttgcagac ctcacacgtg acgtaaacat
      961 cgggcaaaaa gtgcatctcg acgcagcgca ggccgtcgcc cgagcacgcc tcgcagcgcc
     1021 cgcccttcac gttgaacgaa aagcgccccg cgtcgtagcc ataggcacgc gcctccggga
     1081 gccccgcgaa gagctcgcgg atcgagtcga acaccttcgt gtacgttgcc gggttcgaac
     1141 gcggcgtgcg cccgatgggc ttctgatcga tggcgatgac tttgtcgatg tgctcgaggc
     1201 cgtcgatgcc gcggtgctca cccaccggct cgcgcgcgtc gtgcagcgcg cgggagagcg
     1261 ctgggcgcag aataccgttc acgagggtcg atttgccggc gccggagacg cccgtcacgc
     1321 aaaccagagt gccgagcgga aagcgcacgt ccaggttctg caggttgtgc tcgcgtgcgc
     1381 cgcgcaccca gatcgccttg ccgttgccag ggcgccgctc gttcgggagc tcgatgcgca
     1441 aacggccgga aagaaagccg cccgtgagcg accgcggttc gcgcatcacc tgctccggcg
     1501 tgcccgccgc gaccacctct ccacccagca cgcctgcccc cggaccgaag tcgatcaggt
     1561 gatctgcttc gcgcatcgtc tcttcgtcgt gttcgaccac gatgaccgaa ttgccgagat
     1621 cgcgcagcgc cttcagcgtg tcgagcaagc gctggttgtc gcgctggtgc aagccgatcg
     1681 agggctcgtc caaaatgtag atcacgccgg tgagctcgga gccaatttgc gaagcgaggc
     1741 gaatgcgctg gctctccccg cccgagagcg tcgcggccgc gcgatcgagc gtcaggtagc
     1801 cgagcccgac gttcgtcaag aagccgagcc gcgcgctgat ctccttttga agttcctcgg
     1861 cgatgtgcgc gtcggcgccc ttcaacggaa gtttcttgat ccactcgagc gcagcctcga
     1921 tgcgcagcgc cgacacgccg tcgatgccct ggccgtgaat caataccgcg cggctctcgg
     1981 ggcggaggcg cgcgcccttg cacgccgggc acgccttgct cgagaagaag cgcagatagt
     2041 actgcttcat ctcctcgctc tcggtcgcct tcaagcgccg catcatctgg ttgcagagcc
     2101 cctcccactc cgcacgaccg cgcttgccgc cgaacagaat cgcgtcgcgc tggctcttcg
     2161 ggagcttctc ccacggcttg tcgaggtcga tctgaaaagt gtcggcgatc cactcgatcc
     2221 cgctcgcctt ccagccttcc ccgcggctca tcgcgcccgc ccaaacggtg atggccccct
     2281 ggcgaatcga gagcttcgcg ttcggcacga cgaggtccgg atccatctcc ggtcgcgacc
     2341 cgagcccatt gcactcgtga caaaaaccga gcggcgagtt gaacgagaac gcgagcggcg
     2401 caagctcggg aaacgagagc ccgcagtcga cacaggccgc cttctcgctg taggttcgct
     2461 cggcgctgct gccggcgatg atcacggagc cctgcccctc gcgcaacgcc gactcgaccg
     2521 agtccgtcag ccgcgtgcgc tcggccgtcg tcgccgtcac gcggtcgatg accaaatcga
     2581 ggtcgtgctt cttcttcttg tcgagcgaag gctccgtgcc gtcgagcttc accagcttgc
     2641 cgtcgatgcg cgcgcgcacg aagccgcggc tcttgagctc agcgaggcgc tcgaggtgcg
     2701 tgcctttacg ctgccgctcg agcggcgcca aaatcgtgac ctgctgcccc ttctgttcgt
     2761 cgagcagcga gtcgacgatt tgctgcgccg actgcgcgcg cgccggcttc ccgcagctcg
     2821 ggcaatgctg caccccgatg ctcgcgtaga gcacgcgtag gtagtcgtgc acctcggtca
     2881 ccgtacccac ggtcgagcgc gggttactcg aagcgctctt ttgctcgatg ctgatggtcg
     2941 gggaaagccc gcgaatcgag tcgtatttgg gcttctccat ctggcccaag aactgcctcg
     3001 cgtagctcga gaggctctcg acgtagcggc gctgcccttc cgcgtacaac gtgtcgaacg
     3061 cgagcgacga cttcccgctg ccggaaacgc cggtcaggac gaccagctga tgcttcggga
     3121 tctggacggt gacgttcttg aggttgtgtt cgcgcgcacc gcgcacgacg acgagctcgg
     3181 gctctcgact cttcatgggt cagagcggct tagcgctctt tgacctcgct gtcctcttcc
     3241 cgccggcgcg cggcgcggcg cgctcgcgac tcgagcgcat cgatggacgt cgacgccgcg
     3301 agatgcgcga gcatggcttc ctcctccaac gaagagtact cggcattgac gttcagctct
     3361 tcgacctcta ccggaccgtc gtcgcgtggg gcatcttcga aggtcagcac gtggtcgaac
     3421 agcgcgtcag cgttggcgaa ttccggtggg agcagagaga ctctcccgct gtcgaccatc
     3481 gtcttgacgc cgagccgcac cgccgtacgc agagtctcct gcacgccacg cccgtcggcg
     3541 gcgctcgccg acaccacggg cgtgtcctct tcgaggtcta ggcgcttccg gatttccttc
     3601 ggagaaatcg cgccggagac atcttgcttg tttgcctgca cgacgagggg aaccggcgtt
     3661 tcgcgttggc gcatcgacgt ccgaaggcgg gcgaaggtgc gcaacgtatc gtttacctgg
     3721 cgcgggctcg agtcgcacac gaacacgacg acgtccgcca tctccacgag cgggcggcgg
     3781 cggtaatttc gctcgatctg cccgggcacg ctgatgagct ggaacttgag gcttcgccgg
     3841 ttttgcttcg ggccgtccac ctcgacccag tcgaagaaca tggtccgccc cttcaacgcc
     3901 gcgggggtgt acatctcgct gcgcttggtc gtggggacct ggtcgcaaat acgctgcaga
     3961 ttggtggtct taccggcata gccgggaccg tcgtagacca cgcgaaccac gtagcaatcg
     4021 cgagccgaat cgtacgatgc caatctaacc tcgacgcgga tccttccaca gaaccggctc
     4081 cgcggcgaac ggaagttgtc ggccgagcgg cgccgggtcg aggcccgacc gccgctcccc
     4141 ctcgccccac gcggacaacc tgtggatatg tgcgctgccg ctcgtggtcg ggtctcgagt
     4201 tgagtcgccg atctcacgcc gcggcaacgc actgcagagc cgaggcgccg cggacgattg
     4261 tgaccgagcg ctagtcgatg gcgacgccct gacgtgcgcg aagctccaga tattgctcgc
     4321 tcaccgcgta gcccatcaag ttctcgagcc gcgcttcgac gctcgggctt ccctgagccg
     4381 cgggcaccga ctgaaccaga gcaccggcgg tctcgagatc gtgcgcaacc gcgagcccgg
     4441 cgcgatccgc gctgaagtcg acggcgtgaa cccagcgctt caagtcgagc gcggtctcgg
     4501 aagcgagcag cttcgtgacg atgtgcgtca gctgttcgat gccggcgccg gtcgcgagcc
     4561 gctggagcac cttcgaggcg tcggtgatgg gcgcctcgag gtctgccgtt gcggggaact
     4621 tgggctttac gaggcgaatc gccgcaaaca gccaggcttt gagtgcggtg aggttcggca
     4681 agagctgccg caagtagagc ccgggctggt agtacgcgac gtgatggcca gcgatgaacg
     4741 ccgtctgcaa gctatcgaag ttgccgttga aagcgccgcc gccaacgaga acgctcgggg
     4801 gcgcggtcgg taagaagccg agcacgcctg gatcttccgg cttctggaac atggtcggca
     4861 ccggcacgcc cagcgcttcg gcggcgttgt gggtcgcgag cacgagcccg taggggtagt
     4921 tgccgatgtc gattcggtgc tcttcgccga ggccaagcac ctccgggtgc tgtgcgcgcg
     4981 cttggatcac gtatggctcg accagcgcga agagcgcggt gagcagcggg tcggccgtct
     5041 ccggcgcgat gagctccatc cactcttgcg ccgagaggcc ccgcgcgatc tgcggagctt
     5101 cgctgtcgcg catgcgcgag aagaagcgcg tctcgtccgg ctcggcctga ccgagcacgt
     5161 gaagggcctg gcacgcgagg aaggctccgt ccgcgcggcg agcttcggtg tacaccttgc
     5221 ggagcatctt gtacggctgc ggctgatacg gatctcgctt gatccaggcg cccaaagctt
     5281 gcagcgcttg atcgaagtag gccgccggct ccgccgagta gaggagcgcg aggcgctcgc
     5341 tgatgcgcgc gttgtccttc tcgagctcga gcgccgactc gaacacacgg atggcctgat
     5401 ctcgccgcgc gagccgctcg tcgtagatgt cgccgagctc ctcgaggatc tccatgaggt
     5461 tgcccttgtc ctcgcgcgcg gcgaggtctt gggcgcggcg cttgaacatg tccttcaagc
     5521 cttcgaagtc ctgcatctgg cggcgcagct tcacgccttc gctgaacgcc gactcgttgt
     5581 cgggatcggc gtcgagcgcg agatcgatgt acacgagcgc gcgcgctggc tcgcggagct
     5641 cgcgcgccgc gaccatcgcc gccgcataga ggtacttgcc cttctgcttg tcgtcctcca
     5701 ccacctcggc gaggcgcatg atgacctcga tgagctgagg ccagtccttc tcggcgctgt
     5761 agagctgcat caagcgggcg aggatgttgc ggtcgttcgg gcgctcgtcg agcgcgagca
     5821 gcaaggcgcg cgcggcgtag tcggtgttgt gcagctttcc ggccgccact tcgccgattt
     5881 cgatcagggt ctgaacgcgc tcgtcaccct cgacgagctc gagctcgcga taacgcgttt
     5941 cgatgacgcg ctcccactct tcgcgcgcgg cgtaaacttt gccgagcgct cggaacgact
     6001 gcgggctcgc ggcgtcggcg cgaatcgcgc gctcgagcga gtcgaccgcg gcctccacgc
     6061 gcccgagctt gaacagcgct tcgcctcgac ggcgcagcgt cgtcgcgaga tcggtcgggc
     6121 tgagctccgc ctcgtggcgg tcgagcagca cgtcacacac cgcgaaggtg cgctcgggtg
     6181 cgccgtgctc cgcgctgagc tccgaaacgc gcaagagcgc gctcggg
//
LOCUS       DASTKS010000879         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_363559, whole genome shotgun sequence.
ACCESSION   DASTKS010000879 DASTKS010000000
VERSION     DASTKS010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363559"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFQ35_18305"
     CDS             <1..827
                     /locus_tag="VFQ35_18305"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HET9932665.1"
                     /translation="DEAKKLLRNNVAELYTESDAYPIYEGSVGASPREMRGVLLDAAQ
                     NPRFSCLSPFAVLDELDELCERTSEYAFLQEEKLVGGYHDHALFRRTIRERLLDMLED
                     ELRIASGLVDEASYNDLFNRYVTHVSFWVKGEKLRNPLTSQYDEPDERMMREVEGLLG
                     APERPQEFRHGLISTIAAWAIDHPDQPVDNSRVFAGYLKRIREAAFRERRSSVARLCH
                     DIVVLLREEGGGLDEARRAAARRALEQLQSRFGYKDESAADAAAALVRERFHQILP"
     gene            824..2080
                     /locus_tag="VFQ35_18310"
     CDS             824..2080
                     /locus_tag="VFQ35_18310"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9932666.1"
                     /translation="MSAGRVGALSLLALGINGVVGVGIFFTPNLIAELVPGRTGALVY
                     LATGALLVPIALTFSVLATRLGLDGGPYVWARAAFGERVAFGVGWVTAISALLSTAAV
                     LAGLRDHLAPVLHVPAGFARVLFTWACVALLAGIASLGLRPSAWTWDVLTLLKLLPLA
                     ALLFFALLAQPSARPAASGGAPADFGRALLIAVFPLQGFEVVPVLAGSARGRGAIVFA
                     TAGSLLFAAALYSLIQLACVTAAPDLAAHPAPIVEAAKRVGGAPLMGLVSVGTNLSAF
                     ATAFGMIVMTPRYVAALGSDGGFSARLAALDQRSVPRVALLGSAVLIALLASSERLGA
                     LFVLSSSAVLLQYVSSMASLVRLSWRGHAGLSRAWIVPALSSLLAVGFLAKAVDEHEV
                     VALGLTLLAGALAAGLSMRAKRHEPR"
     gene            2097..2876
                     /locus_tag="VFQ35_18315"
     CDS             2097..2876
                     /locus_tag="VFQ35_18315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9932667.1"
                     /translation="MWFDTHCHLDSSNAEGGAGAIISRAEQAGVGYFVCIGVGGTAAT
                     REAVELSESYANVWSTVGVHPHDAASFDGALEAELSSLLDRPRVVALGEVGLDYHYDH
                     SPRARQREVFAHMIAIARQRRKPIVVHTRSAAAETLELLQSESAADVGGIIHCFSEDR
                     AFATRALDLGFDLSFSGIVTFKSARAIQDVALWAPADRILVETDSPYLAPVPLRGRRC
                     EPAFLLHTAAFIASLRGSELSDLRVQTTRNAARRFGLSLEP"
     gene            2900..>3573
                     /locus_tag="VFQ35_18320"
     CDS             2900..>3573
                     /locus_tag="VFQ35_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932668.1"
                     /translation="MRRNRIEPGALAASIAGHCLLVAAGAWLLATHRGVEVPRADEIA
                     VEESGDQQEPGGGAALGEHSVRTERLPDQPKPLHETNARATPRPDMTAQGRGTRSRGE
                     RATNLSSSVDPLTLQRDTPNHLDQSQVQRLRTARRRQSWDDRRATPHPMELDFVASGR
                     GRLALRRPVSDIPPSAGALSPHQPRAVGATPGAASGEGSDGAPGARAAGEAPQVSAGA
                     PGQPSRG"
BASE COUNT          525 a         1220 c         1197 g          631 t
ORIGIN      
        1 acgacgaggc gaaaaagctg ctccgcaaca acgtcgccga gctctacacg gagagcgacg
       61 cctacccaat ttacgagggc agcgtcggcg cgagtccgcg cgagatgcgc ggcgtgctgc
      121 tcgatgccgc acaaaacccc aggttttcgt gcctgtcacc ctttgcggtc ctcgacgagc
      181 tcgacgagct ctgtgagcgc acgagcgagt acgccttttt gcaggaggag aagctcgtgg
      241 gcggttacca cgatcacgcg ctcttccgcc gcacgatccg cgaacggctc ctcgatatgt
      301 tggaagacga actgcgcatc gcgagcgggc tcgtggacga ggcgagctac aacgatctct
      361 tcaaccgcta cgtcacgcac gtaagcttct gggtgaaggg ggagaagctc cgaaacccgc
      421 tcacgagcca gtacgacgag cccgacgagc gcatgatgcg cgaggtcgaa ggcttgctcg
      481 gcgcgccgga gcggccgcaa gagtttcgcc acggtctcat cagcaccatc gccgcgtggg
      541 cgatcgatca ccccgatcag cccgtcgaca actcgcgcgt cttcgccggg tatttgaagc
      601 gcatccgcga agccgcgttc cgcgagcgcc ggagcagcgt ggcgcggctc tgccacgaca
      661 tcgtggtgct actgcgcgag gagggcggag gtctcgacga ggcgcggaga gcggccgctc
      721 ggcgcgccct cgaacagctc caatcacgct tcggttacaa agacgagtcc gccgccgacg
      781 cggccgcggc gctggtgcgc gagcgctttc accagattct gccgtgagcg cggggcgggt
      841 cggggcgctg tccctgctcg cgttgggcat caacggcgtc gtcggcgttg gcatcttctt
      901 cacgccgaac ctcatcgccg agctagtccc gggtcgaact ggtgcgctcg tatacctcgc
      961 gacgggcgcg ctgctcgtcc cgatcgcgct caccttctcc gtcctcgcta cgcgcttggg
     1021 gctcgacggc gggccctacg tctgggcgcg cgccgcgttc ggcgagcggg tcgcgttcgg
     1081 cgttggctgg gtgaccgcga tctcggcgtt gctcagcacg gccgccgtgc tggctggcct
     1141 ccgcgatcac ctcgcgccgg tgctgcacgt gcccgccgga ttcgcccgcg tgctcttcac
     1201 gtgggcttgc gtcgccctcc tcgcagggat cgcgtcgctg gggcttaggc cgagcgcgtg
     1261 gacttgggac gttctcactc tgctgaagct cctgccgctc gcagcactcc tctttttcgc
     1321 attgctcgcg cagccttccg cacgccccgc ggcgtcgggc ggcgcgcccg cagacttcgg
     1381 gcgagcactg ctcatcgccg tcttcccgct ccaaggcttc gaggtggtcc cagttctagc
     1441 ggggagtgct cgcggacgtg gcgccatcgt gttcgcaacc gcgggctcgc tcctgttcgc
     1501 ggcggcgctc tactccttga ttcagctcgc gtgtgtcacc gcggcgcccg atctcgccgc
     1561 gcaccctgcc ccgatcgtgg aggccgccaa gcgcgtcggg ggcgctcctt tgatgggtct
     1621 agtgagcgtc gggaccaacc tttcggcttt cgctactgcg ttcggcatga tcgtcatgac
     1681 cccgcgttac gtggcggcgc tcggcagtga tggtggtttt tcggcgcgac ttgccgcgct
     1741 ggatcaacgc tcggtgcctc gggttgcgct ccttggaagc gcggttttga tcgcgctgct
     1801 ggcgtcgtcg gagcgcctcg gcgcactgtt cgtgctctcg agctccgcgg tattgctcca
     1861 gtacgtaagt tcgatggctt cgctcgtgag gctctcgtgg cgcggccacg cgggtctatc
     1921 acgcgcctgg atcgtcccgg ccctatcgag cctgctggcg gtcggcttcc tcgcgaaagc
     1981 ggtggacgag cacgaagtcg tcgctctcgg cctgacgctg ctcgccggcg cgctcgcggc
     2041 agggctcagc atgcgagcca agcgacacga gccgcgctag attcgctggc gcaacaatgt
     2101 ggttcgatac gcactgtcac ctggactcgt ccaacgccga aggaggcgcc ggcgcgatca
     2161 tttcgcgcgc cgagcaggcg ggggttgggt acttcgtctg catcggcgtc ggcggtacgg
     2221 ccgcgacgcg agaggcggtc gagctctcgg agtcttacgc gaacgtttgg tccacggtcg
     2281 gcgtgcatcc gcacgacgcg gcgtctttcg acggtgcgct cgaggcggag ctctcctcac
     2341 tgctcgacag gccgcgggtg gtcgcgctcg gtgaggtcgg cctcgactat cactacgacc
     2401 actcgccgcg ggccaggcaa cgcgaggtgt tcgcgcacat gatcgcgatc gcacgtcagc
     2461 ggagaaagcc catcgtcgtg cacacccgca gcgccgcggc tgagacgctc gagctgctcc
     2521 aaagcgaatc ggcggcagac gtcggtggca tcatccactg tttcagcgaa gatcgagcct
     2581 ttgcgacgcg cgcgctcgat ctcggcttcg acctctcttt ttctgggatt gtcaccttca
     2641 aaagtgcccg cgcgattcag gacgtcgccc tttgggctcc agcggaccga atcctggtcg
     2701 aaaccgacag cccctacttg gcgcccgtac cgttgcgggg acggcgctgc gaacccgcgt
     2761 ttttgctgca cacggcggcg ttcattgcct cacttcgggg tagcgaactg tccgacctcc
     2821 gcgtccagac gactcgaaac gcagcgcgtc gcttcgggct gtcacttgag ccgtagcgct
     2881 tcgtcgttaa agttcatggg tgcgaaggaa tcgaatcgaa cccggcgctc tcgccgcatc
     2941 gatcgccggc cactgcctgc tcgtggcggc aggtgcgtgg ctcctcgcca cgcatcgcgg
     3001 cgtggaagtt cctcgcgcgg acgaaatcgc agtcgaggag agcggcgacc agcaggaacc
     3061 aggaggaggg gcagcgctcg gtgagcactc cgttcgcacg gagcgccttc ccgaccaacc
     3121 caagccgctc cacgagacta acgcacgcgc gacgcctcga ccggacatga cggcgcaggg
     3181 ccgcggcacg cgcagccgag gcgaacgggc gacgaacctc tcgagtagcg tcgaccccct
     3241 gacgctccag cgcgacacgc cgaaccacct ggaccagagc caggttcagc gattgcgcac
     3301 cgctcgtcgg cgccaaagct gggacgatcg tcgcgcgacg cctcacccga tggagctcga
     3361 cttcgtcgcg agtggtcgcg gaaggctcgc gctacgtcgt cccgtttcag acatcccgcc
     3421 cagcgctggc gcgctgtccc cgcatcagcc gcgcgctgtc ggggctacgc cgggagcggc
     3481 gagcggcgag gggagcgatg gcgcacccgg agcgcgagcc gcgggggaag ctccgcaggt
     3541 ttccgcaggt gctccggggc agccttctcg ggg
//
LOCUS       DASTKS010000880         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1704009, whole genome shotgun sequence.
ACCESSION   DASTKS010000880 DASTKS010000000
VERSION     DASTKS010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704009"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFQ35_18325"
     CDS             complement(<1..309)
                     /locus_tag="VFQ35_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932669.1"
                     /translation="MRRGALTIVPILIGAGAGAALALMQARSHGPAARPADAPQPKTV
                     TAMPVVADSGRSGSEPSGSALAGATQAATSESAAPGADAAQPGAAAPTSNSGIPGTPP
                     S"
     gene            complement(306..2318)
                     /locus_tag="VFQ35_18330"
     CDS             complement(306..2318)
                     /locus_tag="VFQ35_18330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9932670.1"
                     /translation="MENPLLSSARPIPFDAIRAEHVVPAVEALLAEAEQRLASIGSRP
                     PSYESTLAELEAATERLELGMGIVEHLESVATTDALRDAYNAVQPQVTAFWSSIPLRE
                     NVWQALLAFSNTPEASALDATRARLLHKTLAEFRRHGATLDRAGKERLSALDRELGLL
                     TTRFAQNVLDSTNEYELVLPNEHALRGLPALALAQAKESAEAKGKSGYRLTLQAPVVT
                     AVLTYAEDPALREQLWRALNTRGASEPLDNRPLIADILRLRREKAQLLGFANFADLVT
                     DDRMAKTGAQARAFVDELTDKTRAAFERENAELLEFRRRLEGPEAPALRPWDIAYYAE
                     KLRRDRYDLDEEQLREYFSADRALSGAFQVAERLFGVRIEGAKDLPSWDPEVRGYRMS
                     YGTQEIGTFYVDLYPRENKRSGAWMHGLVAAVPPDPHVAIFAANVSPPLGDKPSLLLH
                     RDVETLFHEFGHLLHHCLSRVNVRSLACTRVAQDFVELPSQILENWCVEREALNLFAS
                     HYETSEPLPEALLDRLRAARTFRSANAQMRQLGFASVDLALHVEYDPAKDGDVMRYAR
                     EVLSRYAPAAFPEDYAMVASFLHLFSSPVGYAAGYYSYKWAEVLDADAFSRFRELGVL
                     NPSVGAEFRDKVLARGDSEDPMTLFTDFMGREPRPEALLEREGLAA"
     gene            complement(2409..>2811)
                     /locus_tag="VFQ35_18335"
     CDS             complement(2409..>2811)
                     /locus_tag="VFQ35_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9932671.1"
                     /translation="KSRGNAVLLKASADETAQLLKRAKTDADRQITYDPEARPEVANL
                     LLLASLCSGDAPEAIAARIGDGGGGGLKKLLTELLNETLRPIRARRAELARDAPAVLR
                     RVLSRGTETANRVANATLAEMRGHMNMSYGF"
BASE COUNT          451 a          937 c          923 g          500 t
ORIGIN      
        1 gctggggggc gtgccgggta tcccgctgtt cgaagtcggc gcggcggcgc ccggctgagc
       61 tgcgtcggca cccggcgcag ctgactctga agtggctgcc tgcgtggcgc ccgcgagtgc
      121 cgagccgctg ggctcgctcc ccgaccttcc gctatctgcg accacgggca tagccgtgac
      181 ggttttgggc tggggggcgt cggcagggcg cgcggcgggc ccgtgcgagc gcgcttgcat
      241 cagcgcgagc gctgcccccg cgcccgcacc gatcaggatc ggtacaatcg tgagcgcgcc
      301 tcgcctcatg cagctagacc ttctcgttcg agcagggctt ccggtctcgg ttcgcgaccc
      361 atgaagtccg tgaaaagggt cattggatct tcgctatccc cgcgtgccaa aactttgtcg
      421 cggaactctg cgccgacgct cgggttcagt accccgagct cgcgaaatcg gctgaacgcg
      481 tcggcatcca acacttccgc ccacttgtac gagtaatagc cggcggcgta acccactggg
      541 ctcgagaata ggtgcagaaa gctcgctacc atcgcgtagt cctcggggaa cgccgcgggc
      601 gcatagcggc tgaggacctc acgcgcataa cgcatcacgt ccccatcctt ggccgggtcg
      661 tactcgacgt gcagcgccag atccacgctc gcgaatccga gctgacgcat ctgcgcgttc
      721 gcggaacgaa aggtgcgcgc cgcgcgcaaa cgatcgagca gcgcctccgg cagcggttcc
      781 gaggtttcgt agtgcgaagc aaagaggttg agggcttcgc gctcgacaca ccagttttcc
      841 aggatttggg acggcagctc cacgaaatcc tgcgccacgc gcgtgcacgc caagctacgc
      901 acgttcacgc ggctcaggca gtggtgcagc aagtgaccaa actcgtggaa cagcgtctcg
      961 acgtcgcggt gcagcaagag cgagggctta tcacccagag gggggctcac gttcgctgcg
     1021 aagatggcca cgtgcggatc ggggggtacc gccgcgacga gcccgtgcat ccaggctccg
     1081 ctgcgcttgt tttctcgcgg atagagatcc acgtagaagg taccgatctc ttgcgtgccg
     1141 tagctcatcc ggtaaccccg gacctccggg tcccagctcg gcaagtcttt ggcgccctcg
     1201 atccggacgc cgaataggcg ctcggcgact tggaacgcac ccgacagtgc ccgatccgcc
     1261 gagaagtact cgcgcagttg ctcttcatcc aggtcgtagc ggtcgcggcg caacttctcc
     1321 gcgtagtagg cgatgtccca ggggcgcagc gcgggcgcct ctggcccttc gagtcgccga
     1381 cggaactcga gcagctcggc gttctcgcgc tcgaaggcgg cgcgcgtctt gtcggtgagc
     1441 tcgtccacga aggcacgcgc ctgcgcgccg gtcttggcca tgcgatcgtc cgtgacgaga
     1501 tccgcgaagt tcgcgaaacc gaggagctgc gccttctctc ggcgtagcct tagaatatcg
     1561 gcaatcaacg ggcggttatc gagcggttcc gaagcacctc gagtgttgag cgcgcgccac
     1621 agctgctcgc gcaacgcggg gtcctccgcg taggtcaaca ccgcggtcac gaccggtgcc
     1681 tgcagggtca ggcggtaccc cgatttgccc ttggcctcgg cgctctcctt cgcttgcgcg
     1741 agcgccaatg cggggagccc gcgcagtgcg tgctcgttcg ggagcaccag ctcgtattcg
     1801 ttcgtcgagt cgagcacgtt ctgcgcgaat cgcgtggtca gcaaacccag ctcgcgatcc
     1861 agggccgaga ggcgttcctt cccggcccga tcgagcgtag cgccgtgccg gcggaactct
     1921 gccaaagtct tgtgcagcaa acgggcgcgc gtggcgtcta gcgcggatgc ttccggcgtg
     1981 ttcgagaacg cgagcaacgc ttgccacacg ttctctcgca gcgggatgga ggaccagaag
     2041 gccgtgacct gcggctgaac ggcgttgtag gcgtcccgca acgcgtccgt ggtcgcgacg
     2101 ctctccagat gttcgacgat ccccatgccc agctcgagcc gctccgttgc cgcctcgagc
     2161 tccgcgagcg tgctctcgta gctaggtgga cggctcccaa tcgaggccaa gcgctgctcg
     2221 gcttccgcga gcagcgcttc cacggctggc accacgtgct ccgcccggat cgcgtcgaag
     2281 ggaatgggtc gtgcagacga caggagcgga ttttccatgt gaatcgaggc tttctatacc
     2341 acgccggttt gcgctggtcg gtccgaggcc gaaacgaggt tcgctccacc gacgcgagcg
     2401 catgagatct agaacccgta agacatgttc atgtggccgc gcatttcggc gagcgtggcg
     2461 ttcgcgacac gattggcggt ctcggtgccg cgggatagca cccgacgcaa tacggccggc
     2521 gcgtctcgag ccaactcggc ccggcgcgcc cggatcgggc gcaacgtttc gttcagcagc
     2581 tccgttagta acttcttcaa gccgccgccc ccgccatcgc cgatgcgggc cgcgattgct
     2641 tcgggcgcgt ccccgctgca tagggacgcc agcagcagta gattcgcgac ctcgggccgc
     2701 gcctcgggat cgtaagtaat ctggcgatcg gcgtcggtct tggcgcgctt cagcagctgt
     2761 gccgtctcgt ccgcgctggc cttcagcagc accgcattgc cgcggctctt g
//
LOCUS       DASTKS010000881         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_810299, whole genome shotgun sequence.
ACCESSION   DASTKS010000881 DASTKS010000000
VERSION     DASTKS010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810299"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VFQ35_18340"
     CDS             complement(<1..1459)
                     /locus_tag="VFQ35_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932672.1"
                     /translation="MAYRLKRGPKVFAALVLSGAAFAGVRYALQSGLIGPTGAGSKLP
                     SRVELPVLAEAPSEALPAPVAMPGVDRGCPELPETRALIWAWNAQMGWMFANGGVEST
                     NGSLMCRAGVKMKLVRQDDAEQMKSELLKFATALKRGEPDPSSGTHFVAIMGDGTAQF
                     LASLNPQLARLGPEYRAKIIGSAGYSRGEDKFMGLPAWKQNPGAARGAYVAGYLKDGD
                     WNIALKWAGDNGICNNPDEHVYDPNCLNWAGSKDYLDAAQKYVEGYCDDLPIRGNLGN
                     RKHVCVNGVVTWTPGDVIVAEKRGGLVSIVSTREYASQMPNAVIGVEPWLGRNRARVE
                     GFLSAMLEGGRAVKSSGEALRRAAEISNQVYAEKDTGADYWLKYFKGSRERDKQGLEV
                     ELGGSKVNDLADNVALFGLAPGYANAFEATYTTFGNIVREQYPNDVPSFPPAAEVIDT
                     SFLKALASRQSQAPRPSEESVAYAAVAAAPDHTELG"
     gene            complement(1552..2556)
                     /locus_tag="VFQ35_18345"
     CDS             complement(1552..2556)
                     /locus_tag="VFQ35_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932673.1"
                     /translation="MSNLTVNSPKSFLQRPEGKVGAAMSVALVAALGYGAYVVLPVLI
                     TLVTNVLYLGALCAALGGALYLCLDRRVRAFLSYAYRAFWRWLVGRVIEIDPIGIMKS
                     HIAELGNKLTVMREQLGALHGQVRQIERVMQENSAQRDGAMQLALTAKGAPEHASAGR
                     LSARRAVKLEESNDNLATMKAQLEALFRVLKKLYDASEMVLSDMQSTVDIKSRERASM
                     KAAYSVYRSAFAILQGESEGREMYDRALEFLNEDYAQKLGEIEMFMDFSDGLIKGADL
                     QKLSYDVEAARKLDAWEARLSNGLLASGVPTPDGGASESCADVDPLESLLEGTRAKAR
                     "
     gene            complement(2556..3284)
                     /locus_tag="VFQ35_18350"
     CDS             complement(2556..3284)
                     /locus_tag="VFQ35_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932674.1"
                     /translation="MNRHDDDVVSACGRVEDARVQAPVDSSGFMSRVRRVLFEQETSA
                     NSKEQPRPAPDTTATSREPDLVSGITATLRDLVTRDVGPALAEFNLQIEAVREVVPER
                     STQMKIALSVLSKKGIALRELERDLALVGELLAQQLESFSEKVAARRREHVANVEQVE
                     LESREARARAENEVVALEAALKTQRDCLVAINAQREKRLGEISIAEREIEQKERAFRS
                     AQAGLLGEYERLGRDLAPLLAEKV"
BASE COUNT          685 a         1259 c         1143 g          743 t
ORIGIN      
        1 gtccgagctc ggtgtggtcc ggcgcggccg cgaccgcggc gtaagctacg ctctcttcgc
       61 tcggccgcgg cgcttggctc tgccgcgacg cgagcgcctt caggaaagac gtatcgatga
      121 cctcggccgc gggagggaag ctcggtacgt cgttcgggta ctgctcgcgc acgatgttgc
      181 cgaacgtggt gtacgtcgct tcgaacgcgt tcgcgtagcc cggggcaagc ccgaagagcg
      241 ctacgttatc ggccaagtca ttgaccttgg agccgccgag ctcgacctcg agaccttgct
      301 tgtcgcgttc acggctaccc ttgaagtact tgagccagta gtcggcaccc gtgtccttct
      361 cggcgtacac ttggttactg atctccgccg cgcgacgtag cgcctcgccc gaggatttaa
      421 cggcccgtcc gccttccagc atcgcgctca aaaacccttc gacgcgggct cggttccggc
      481 cgagccaggg ctcgacgccg attacggcgt tcggcatctg cgaggcgtac tcccgcgtcg
      541 agacgatgga cacgagccca cctcgcttct cggcgacgat gacgtcgccc ggagtccacg
      601 taacgacccc gttgacgcag acgtgcttgc gattcccgag gttgccgcga atcggcagat
      661 cgtcacagta cccttcgacg tacttctggg ccgcgtcgag gtagtctttc gaaccagccc
      721 agttcagaca gttggggtcg tagacgtgct cgtcggggtt gttgcagatc ccgttgtcgc
      781 cggcccactt gagggcgatg ttccagtcgc cgtctttgag atagccagcg acgtaagcgc
      841 ctcgcgcagc gccggggttc tgcttccagg cgggtaggcc catgaacttg tcttcgcccc
      901 gcgagtagcc ggctgagccg atgatcttcg cccggtactc tgggcccagg cgcgcgagct
      961 gcgggttcag cgacgccaag aactgcgccg taccgtcacc catgatcgca acgaagtggg
     1021 tgccgctcga cggatcgggc tcgccgcgct tgagcgccgt cgcgaacttc aagagctcgc
     1081 tcttcatctg ctcggcgtcg tcttgacgaa cgagcttcat cttgaccccg gcacgacaca
     1141 tcaagcttcc attcgtcgat tcaacgccgc cgttcgcgaa catccatccc atttgcgcgt
     1201 tccaggccca aatcaacgct cgcgtttctg gtagctcggg acagccccga tcgacgcccg
     1261 gcatcgcgac cggcgcgggg agagcctcgc tcggagcctc ggccaaaacc ggcaactcga
     1321 cgcgcgacgg gagcttcgat ccggctccgg tcggcccgat gagcccgctc tgcagcgcgt
     1381 agcggacgcc cgcgaacgcg gcgcccgaca agaccaacgc agcaaacacc ttcggaccac
     1441 gcttcaatct gtaggccaca atgatttcct ttcgttgcaa tctggttcac gcgacaaagc
     1501 tctcgcctga ggcgatgctt cgcgcccaaa gttccgttcc gagaaccgaa gtcagcgcgc
     1561 cttcgcgcga gtgccttcga gcaatgactc gagcggatcc acgtcggcgc acgactcgga
     1621 cgcgccgccg tctggagtag ggacgccgct cgccaaaagt ccgttcgaca agcgtgcctc
     1681 ccatgcatcg agcttccgcg cggcttcgac gtcgtacgag agcttctgaa ggtccgcgcc
     1741 cttgatgagg ccgtcggaga aatccatgaa catctcgatt tctcccagtt tctgcgcgta
     1801 gtcttcgttc agaaattcga gcgcacggtc gtacatctcg cggccctccg actcgccttg
     1861 gaggatcgcg aatgccgaac ggtacaccga gtaagcggct ttcatgcttg cgcgttcgcg
     1921 cgacttgatg tccaccgtac tctgcatgtc ggagagcacc atctcggacg cgtcgtaaag
     1981 ctttttcagg acgcgaaaga gagcttcgag ctgcgctttc atcgtggcga ggttgtcgtt
     2041 cgactcctcg agtttcacgg cgcgacgcgc cgaaagcctt cccgccgacg catgctcggg
     2101 ggcacctttc gcggtgaggg cgagctgcat cgccccgtct cgctgcgccg agttctcttg
     2161 catgacccgc tcgatttgac gcacctgccc gtgcagggca cctagctgct cacgcatgac
     2221 ggtgagcttg ttgccgagct cggcgatgtg gcttttcatg atgccgatgg gatcgatttc
     2281 gatcacgcgc ccgaccagcc aacgccaaaa cgcccgataa gcgtagctca agaaagcgcg
     2341 caccctgcga tcgaggcaaa ggtagagcgc cccacccaag gccgcgcaca gcgcgccgag
     2401 gtagagcacg ttcgtaacca gcgttatcag gacgggcagc acgacgtacg cgccgtagcc
     2461 aagcgccgcg acgagcgcga cgctcattgc ggcgccgact ttgccttccg gacgctgcag
     2521 aaatgacttc ggtgaattga cagtgaggtt cgacatcaga ccttctccgc cagcagcggg
     2581 gctagatcgc gcccgagccg ctcgtactcg ccgagcaagc cagcctgtgc actcctaaag
     2641 gcgcgttctt tctgctcgat ctcgcgctct gcaatcgaga tctcgccgag gcgcttttcg
     2701 cgctgcgcgt tgatcgcgac caagcagtct cgttgagttt tgagcgccgc ttcgagcgcg
     2761 acgacctcgt tctcggcgcg cgcgcgagcc tcacggctct cgagctcgac ttgctcgaca
     2821 ttggccacgt gctcacgtcg ccgtgcggcg accttctcgg agaacgactc gagctgttga
     2881 gcgagaagct cgcccacgag agctagatcg cgctcgagct cacgcaaagc aatccccttc
     2941 ttggacaaca ccgagagcgc gattttcatc tgcgtcgaac gctcggggac cacctccctc
     3001 acggcttcga tctgcaagtt gaactctgcg agcgcaggcc ccacatcgcg agtcacgagg
     3061 tcgcgtaggg tcgcggtgat cccgctcacg aggtcgggct ctcggctggt cgccgtggtg
     3121 tcaggcgcgg gccgaggttg ctctttcgaa tttgcgctcg tttcctgctc gaacaagacc
     3181 cttcgaacgc gactcatgaa acccgatgaa tcgacgggcg cctgcacgcg cgcgtcctcg
     3241 acgcgtccgc acgcagacac gacgtcatcg tcatgcctat tcatggactg ctcctattgg
     3301 gaacgatcga cgcgcgaact cgtctcgcga ggtgattcgg cgcgccgatg cccaggcaaa
     3361 aaagaaggac gtttcaacgc ctcgagcggc gaggcggcac ggtcgttgtt ttctacatgg
     3421 gcgtaccagg gagttctgcg cgcgaagcga cgcagccctg caccaacggc aggaagtgtt
     3481 gccacctgcc gcagagcgcg caatagggtc gcgagcgatt tttagccgat tttcttgcag
     3541 cgtcccccga attcgaacgc gctaagagga gagttcagtt cctcaagagc gacttgcatc
     3601 agccgttcca acagcgtcgc tgaataacga gcaattcact ggcatcgaca ctggagagct
     3661 cggtttcgct cggccgacca atccttggca tttcaagttc atcgattcac tgggagttct
     3721 ggtgttccgg tgtgatgacg aatccatcgt ccccgcagac acgaactctc tcggtcgcgt
     3781 cacccgagag agcaacccgg agcggcgcga ccttgagcgg agtcatcgcg
//
LOCUS       DASTKS010000882         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_142282, whole genome shotgun sequence.
ACCESSION   DASTKS010000882 DASTKS010000000
VERSION     DASTKS010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142282"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFQ35_18355"
     CDS             <1..1260
                     /locus_tag="VFQ35_18355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9932675.1"
                     /translation="RVEVLEGPDQGRVIETEAELLSVGTAVGNALKLSDDTVSRFHLE
                     LSRRADGVYVEDLSSSNGTLLGSARIERGVIQPGSELVLGRTRLVVGEGAESSVELFE
                     GGELDDLKGQTEVMRRLMAQVTTAARSNAPALVIGESGTGKELVARALHDHGPRANGP
                     FVTLDCGALAPNLVASELFGHERGAFTSAERQHIGAFERADKGTLFLDEIGELPAELQ
                     PQLLGALERRRFRRVGGRSDVSVDVRIVAATNRDLRAEVNSGTFRLDLYYRIAVVVLR
                     LPALRERTGDIPILVRHFLRECGYEGELERLISDAKLAELALHRWPGNVRELRNWVEA
                     TVAMGESPELAPAVTLPPEAGELNDQWLDRSYKDARRLVLEDFERRYLKHILEATTGN
                     VAQAARKAQMDRTYLIKLIQRHAMRRR"
     gene            1409..2455
                     /locus_tag="VFQ35_18360"
     CDS             1409..2455
                     /locus_tag="VFQ35_18360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932676.1"
                     /translation="MDTALSAWLGERAIACEVNGSRIVAATSAFARCFELAEPDCIGR
                     EFSSLFSDTDARVSGLKLDDARAQRREVSFLSKHWRASGARIWLRWHLDARASGNVRA
                     LAFDVTHERVLDDECAQLRHMLEHSQRLAAIGALANRIVHDLNHLMLAMQQGVELSLA
                     TIAQGHPSHEDLTAVQACLATAVGLTSEIRRHGGAARAELELIDLAELIRAMQPVLAL
                     ATRPDTRLVVECIPGTVPIYGHAVQIRQVLLNLVVNASEAIGARPGRIVVRVEPTLDA
                     SGTQGVCVSVVDDGCGMSPETKARLFEPFFSTKSSSRGLGLSGTLAILEAHHAQVSVQ
                     SELGRGTKVEVRFP"
     gene            2574..3161
                     /locus_tag="VFQ35_18365"
     CDS             2574..3161
                     /locus_tag="VFQ35_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932677.1"
                     /translation="MGCVARLSLGFGSMAVLGFAMACGEATRNAQAGTGGAQASSASS
                     ASGGANASGGVTANGGSRALSSGGAATCDVGVDPCPADTTELVDRSDTSNCLLRKSLD
                     CDREAFDAELQEVGGALFEPCAAVEPHDGCGRLLLDFDANGCLSSLTTPIEPDEGLRD
                     HLGALRACLKPIFEDARFACLPSAHLEYRESCFVR"
BASE COUNT          559 a         1090 c         1110 g          627 t
ORIGIN      
        1 cgcgtggagg tcctggaggg gccagatcaa ggccgcgtga tcgagaccga ggccgaactc
       61 ttgagcgtcg ggaccgccgt tggcaacgcg ctgaagctca gtgatgacac cgtgtcgcgc
      121 tttcacctgg agctctcacg ccgagcggat ggggtctacg tcgaggacct ttcgtcgagc
      181 aacgggacct tgctcggcag cgcgcgcatc gagcgtggcg taatacagcc cggaagcgag
      241 ctcgtcctcg gccgcactcg tctcgtcgtt ggagagggag ccgagagctc cgtcgagctc
      301 ttcgaaggcg gcgagctcga cgatctaaaa ggccagaccg aggtgatgcg gcgtctgatg
      361 gcgcaagtca cgaccgccgc tcgctcgaac gcacccgcgc tcgtgatcgg agagtcgggt
      421 accggcaaag agctcgtcgc gcgcgcgctg cacgatcatg gcccgcgcgc gaatggtccc
      481 ttcgtcaccc tggattgcgg cgccctggct ccgaacctcg tcgcgagcga gcttttcggg
      541 cacgagcgcg gcgcgtttac cagtgcagag cgacagcaca tcggcgcctt cgagcgtgcc
      601 gacaagggca cgttgtttct cgacgaaatc ggtgagctcc cggccgagct ccaaccccaa
      661 cttctcggcg cgctcgagcg gcgccggttc cgccgtgtgg gaggacgcag cgacgtgagc
      721 gtcgacgttc gcatcgtggc cgcgaccaac cgcgacctgc gcgccgaagt caattctggc
      781 actttccgac tcgatctcta ttaccgcatc gcggtggtgg ttcttcgcct gcccgcgctt
      841 cgcgagcgca ccggcgacat tccgattctg gttcgccact tcttgcgcga gtgcggttac
      901 gagggggagc tcgaacggct gatctccgac gccaaactcg cggagcttgc gcttcaccgc
      961 tggcccggca acgtacgaga gcttcgcaac tgggtcgaag cgacggtggc gatgggagag
     1021 agccccgagc tcgcgccggc agtgacgcta ccgcccgaag ctggtgagtt gaacgaccag
     1081 tggctcgacc gctcgtacaa agacgctcga cgactcgtgc tcgaagactt cgagcgccga
     1141 tatttgaagc acattttgga agcgacgacc ggtaacgtcg cgcaggcagc gcgaaaagca
     1201 cagatggatc gcacgtattt gatcaagctg atccaacgcc acgcgatgcg ccgtcgttga
     1261 atggttgtcg tctgcagacg acagtgcagg ctcgagacga cactcgagca agtccgagcc
     1321 tcgtactttc ggggccttcc ggggcccggt ccccggctgt agaggtgccg aacttcggca
     1381 cggcggttgc tgtcgatggc ggcgaaagat ggacaccgct ctctcggcgt ggctcggcga
     1441 gcgcgcgatc gcgtgcgaag tcaacggttc aagaatcgtg gccgcgacct cggccttcgc
     1501 tcgttgcttc gaactcgcgg aacccgattg cattggtcgc gagttctctt cgctcttttc
     1561 tgataccgac gctcgcgtga gcgggctcaa actcgacgac gctcgcgccc agcggcgtga
     1621 ggtttcgttc ctgagcaagc actggcgcgc ttcgggggct cgcatctggc tacgctggca
     1681 cctcgacgcg cgcgcgtcag gtaacgttcg cgcgctggcg ttcgacgtga cccacgagcg
     1741 cgtgttggac gacgagtgcg cccagctgcg tcacatgctc gagcatagcc aacggctcgc
     1801 ggccatcggc gcgctcgcga accgcatcgt tcacgatttg aaccacttga tgctcgcgat
     1861 gcagcaaggc gtcgagctct ccctcgcgac gatcgcgcag ggccacccat cccacgagga
     1921 cctcacggcc gttcaggcgt gcctggccac tgcggtgggc ctcaccagcg agattcggcg
     1981 gcacggcggc gcggcgcgcg cggagctcga gctcatcgat ctcgcggagc tgattcgtgc
     2041 gatgcagccg gtgctggcgc tcgcgacccg ccccgatacg cgactcgtcg tcgaatgcat
     2101 tcccggcacg gtgccaatct atggacacgc ggttcagatt cggcaggtct tgctgaactt
     2161 ggtcgtcaac gcgagcgaag cgatcggcgc gcgcccgggt cgcatcgtcg tgcgcgtcga
     2221 gccaaccctc gacgcgagcg gcacccaggg cgtgtgcgtc agcgtggtcg acgacggctg
     2281 cggcatgagc cccgagacca aggcgcgctt gttcgagccc ttcttttcga ccaagagctc
     2341 gagccgcggc ctcggtttgt ccggcactct cgcgattctc gaagcgcatc acgctcaggt
     2401 cagcgtccaa agtgagctcg gccgcggcac gaaagtcgaa gtccgctttc cctgatagag
     2461 ctgcttctgc gcttcgtgtg tgcctggcat tggtctgtcg tgacgcgggg cgtttccgcg
     2521 ccctaagcgc tttcgcggca gcggcctcgt tcggtcgtgg taccctgacc tgcgtgggat
     2581 gcgtggcgcg gctctctctt ggctttggct cgatggcggt gctcgggttt gcgatggcgt
     2641 gcggtgaagc cacgcgcaac gcccaagcag gaaccggcgg cgctcaggca tccagcgctt
     2701 cgtccgcatc cggcggtgcg aacgcgagcg gtggcgtgac cgcaaacggg ggcagccggg
     2761 cgctctcgtc tggcggagca gctacgtgcg acgtcggcgt cgacccctgc ccagcggata
     2821 cgaccgagct cgtggatcgc agcgacacgt cgaactgcct tttgcgaaag agcctcgatt
     2881 gcgaccgtga ggctttcgat gcagagctgc aagaggtggg cggcgccctc ttcgagccat
     2941 gtgcggccgt tgaaccacac gacggttgtg ggcggctgct gctcgatttc gacgccaacg
     3001 gttgcttgag tagtctgacg actcctatcg agccggacga aggccttcga gatcacttgg
     3061 gggcgttgcg cgcctgcctc aagcccattt tcgaggacgc tcgtttcgcc tgcttgccga
     3121 gcgcgcacct cgaataccgc gagagctgct tcgtccgctg agccaacgag cgcgctctcc
     3181 gcgcctccga ttggacaacg gaggcgcgac gatgttctct ccaatgttgc ctttgagcga
     3241 caacgccagc gcggggacga accttccctg ggctcagcgc gattttcgaa agagtcagaa
     3301 cgcggagctc ctttacgcag agccgaattc gaaatttcgt acatcgttct tcggagccga
     3361 ccaaatcttt tgccgactcg gctcgt
//
LOCUS       DASTKS010000883         7663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1034464, whole genome shotgun sequence.
ACCESSION   DASTKS010000883 DASTKS010000000
VERSION     DASTKS010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034464"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..427)
                     /locus_tag="VFQ35_18370"
     CDS             complement(20..427)
                     /locus_tag="VFQ35_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932678.1"
                     /translation="MTETPAQASALNVLIVEDHADSGYVLHEFVHFMGYTSVLAESAE
                     EALSKLESYTPHVIFLDILLPGRSGYDFAAELRRRFGDGVFLVAFTGIVGPAAESKAK
                     EAGFDLFLTKPVGLDDVRDVLAVRAAALRLASV"
     gene            complement(631..3096)
                     /locus_tag="VFQ35_18375"
     CDS             complement(631..3096)
                     /locus_tag="VFQ35_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9932679.1"
                     /translation="MLIVDDEPQVLVALEDLLSEFFDVVATDDPARALRLAEQEPDLA
                     VILSDERMPHMNGHELLARLRERCSAARLLVTGYADLQAIMRAINEGQIFAYVTKPWD
                     APDLTNKVVRAAEHFRLTQDLADERQLLHGLMNSMPDAIYFKDREHRFVRVNRGFLDM
                     FGVADESRVIGARLSEMLDGSPVAHALELAEKPVLLGTEARCNAEHPHPRRGDDEPAW
                     LSTTNAAIVSPSGAVIGLVGIARDVTDRVRAQSALQASEERLRLAFAASNAILFDCDL
                     GTGEVSLLGPKDGPVPAAGARFDVAELERLVHPEDRSRVAEALSKHLQSRAPFQALEL
                     RALADSGEYRWFEIHGQATWDESGKALRLVGSSVDITTRKEHAAQAARLAFLSSHDEV
                     TGLPNRQLLATELERRIALNRTSNTGAGALALFDLSRLRQVNESFGRQVGDDLLREMG
                     ARLSALAEANDLVARLDSSMFAVLLDRADAGKVGEWLRHVSVRLSQAFTLRPGELRVA
                     VAAGVALFPNDGASAEELVAKARAAQEAAKRSGRAHCFYAPSMSSSTAERLKLESNLR
                     RALVRSEFVLFYQPKVELRTGRVVGVEALLRWKDPERGLIAPGAFIPVLEETGMIADV
                     GSWVLAEAARQYTQWKSAERTPPRIAVNVSALQLQQPNFLETLDAAISKHPEARHGLD
                     LEITESVLAGDVEDNIQKMRAVKERGFKIAIDDFGTGYSSLRYLSRLPLDALKVDRTF
                     VDAMAEDPQQMTIVTSIISLAQALGLKVIAEGVETTMQAHLLRLLRCDEMQGYLVSKP
                     LPAPEAEPLLARVFEVQGSFAKT"
     gene            complement(3636..4430)
                     /locus_tag="VFQ35_18380"
     CDS             complement(3636..4430)
                     /locus_tag="VFQ35_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932680.1"
                     /translation="MSRGHHLSDFAHAERWNGSVAPSEPTLAEISRLVEGPIASIVAN
                     IEFASSVLAGVADLASMDGGKASAVALGSVTQALAQAATSTTELRSLVRRLRDGESSK
                     DGVRKTNGRPPARRESGVQARPGARPSQGGYGEHVPRILVVDDEVAVGRTIQRILRDR
                     YDVTFESNSSTAAYQLLLGEDYDVIVCDVVMPTMSGIDLFKVVSAARRELAARFVFMS
                     GGVHSPEALSFFEKGPNVLINKPFTTARVVAAIDSVLHLHAQGCGT"
     gene            5019..5438
                     /locus_tag="VFQ35_18385"
     CDS             5019..5438
                     /locus_tag="VFQ35_18385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9932681.1"
                     /translation="MAKLVRELMSKPLALPSSAPVVEAARQMQNEDVGTVIVEDGGKP
                     FGIVTDRDIVVRVVAEGRDPAQTAISEVCSKELVTLSPDAELDLAVQLMRDRAVRRLL
                     VVNGEHRTVGLISLGDLAIERDPNSVLGQISATPPNS"
     gene            5479..5952
                     /locus_tag="VFQ35_18390"
     CDS             5479..5952
                     /locus_tag="VFQ35_18390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9932682.1"
                     /translation="MKVRELMTVNVARVASDEPLSSAARLMWDCDCGAVPVTDSINHR
                     VIGILTDRDICMATWIRDKPPSEIPVREAMSRDLFYCSPDQSISSAESLMRAKQVRRI
                     PVLDDDKNLLGILSLADIAKQSQREGLRGASALAPEEVSTTLAQICERRDFARAG"
     gene            6298..6834
                     /locus_tag="VFQ35_18395"
     CDS             6298..6834
                     /locus_tag="VFQ35_18395"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9932683.1"
                     /translation="MIVPTNQYVSFRLAAETYAIDVSHAKEIVEAPRITALPHSPPWI
                     RGVMNLRGSVVPVVDLKSKFAMGTTELTAHTCALLVEFEAEEGRFLIAVLVDAVLAVF
                     ELLANELESAPAFGARYSRKYLKGVGRREGVLFMVLDANEVFADVEPELGDAPAENDE
                     REPAPPLSGPRPEDAPSL"
     gene            6858..>7663
                     /locus_tag="VFQ35_18400"
     CDS             6858..>7663
                     /locus_tag="VFQ35_18400"
                     /inference="COORDINATES: protein motif:HMM:NF014281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HET9932684.1"
                     /translation="MQWFNNLKLAVKLTSCFILLAILVGLVGNVGRSSTNELGGRVQL
                     MKENVIEAIVNLRDAERQMTLYRGDVWKLLADEGAPHGELIREIEARPAAVERALQKY
                     DSRTKFPGEAELVQEFRSKFAELTAIRNRAVSMEATAHHDAVRLMRDSTPTEKAARAA
                     LSKLIELNVEQARVQSSAAVETSHQASRQILLASVIAAALAIGLGLLLSRSLTWAIGE
                     VANAAERIAAGELDVEIALQQKDEIGHLAQTQRRMTAQLRQTVMQIQAVA"
BASE COUNT         1439 a         2470 c         2433 g         1321 t
ORIGIN      
        1 gggcggtgcg ttcttccgtt cacacggatg caaggcggag cgcagctgct cgcacggcga
       61 gaacgtctcg gacgtcgtcc aaacccaccg gcttcgtgag aaagaggtcg aaaccggcct
      121 ctttcgcttt gctctccgcg gcgggcccca cgatcccggt gaacgcaacg aggaacacgc
      181 cgtcaccaaa gcgccggcgg agctccgctg cgaaatcgta gccggacctc cctggcagga
      241 ggatatcgag aaatattacg tggggcgtat aactctcgag tttggacaag gcctcttcgg
      301 cggactcggc gagcaccgac gtgtagccca tgaagtgcac gaactcgtgg agaacgtacc
      361 cgctgtcggc gtggtcctcg acgatcagaa cattcaaagc gctcgcttgt gcgggcgttt
      421 cggtcatcag atattccaag ccgtaataag tcgcgggcct tgagccgttg agaccgtctc
      481 gccccgcgga gcgaacgtga cttccgagcg ccctagtttc gaactcctaa ggccgccaga
      541 ggccaaagtc catggtcatg acctcgagga aggcgcattc ggcgaaccct ttccaacccc
      601 ataggctaaa ccgcgcacac cctttcagct tcacgtcttc gcgaacgacc cttggacctc
      661 gaagactcgt gcgaggagcg gctcggcctc gggggcgggc agcggtttgg acacgaggta
      721 cccctgcatc tcatcgcagc gaagaagccg cagcaggtgc gcttgcatgg tggtctcgac
      781 gccttcggcg atgaccttga gccccagcgc ttgcgcgagc gaaatgatgg acgtgacgat
      841 ggtcatctgc tgcggatctt cggccatcgc gtcgacgaag gtgcgatcga ccttcagcgc
      901 gtcgagtgga agccggctca gatatcggag agacgaatag ccggtcccga agtcgtcgat
      961 cgcgattttg aagccacgct ctttgaccgc cctcatcttt tggatgttgt cttcgacgtc
     1021 tccggcgagc acgctctcgg tgatctcgag atcgagtcca tgtcgagcct ccgggtgttt
     1081 ggatatcgcg gcgtccagcg tttcgaggaa gttcggctgt tgcagttgaa gcgcggacac
     1141 gttgacggcg atgcggggcg gcgtacgctc ggcgctcttc cactgagtgt actggcgagc
     1201 ggcctcggcg agcacccaac ttccgacgtc ggcgatcatg ccggtctctt cgagcacggg
     1261 gatgaacgcg ccaggcgcga tgaggccacg ctccggatcc ttccagcgca gcagcgcttc
     1321 cacgcccacg actcgcccgg tcctcaactc gactttgggc tgataaaaca gcacgaattc
     1381 gctgcgcacg agcgcgcgcc gtaagttcga ttcgagcttc agccgctccg cggtgctgct
     1441 gctcatgctc ggggcataaa agcagtgcgc gcgcccgctg cgcttggcgg cttcttgagc
     1501 cgcacgagct ttcgcgacga gctcctcggc gctcgctccg tcgttcggaa agagcgcgac
     1561 acccgcggcg accgcgacgc gcagctcgcc gggtcgcagc gtgaacgcct gggacagtcg
     1621 caccgacacg tgccgcaacc actcccctac tttcccggca tcggcgcggt ccaacagcac
     1681 cgcgaacata ctgctgtcga ggcgggcgac caagtcgttc gcttcggcga gggcggacag
     1741 ccgcgcgccc atctcgcgca gcaagtcgtc gcccacttgt cgaccgaagc tctcgtttac
     1801 ctggcgaagg cgagacaagt cgaacagggc caacgcgcca gcgccggtgt tgctggtccg
     1861 gttcagcgca atacgccgtt cgagctcggt cgcgagcagc tgcctattcg gcaggcccgt
     1921 gacctcgtcg tgcgagctga gaaaggcgag ccgggccgct tgagctgcat gctctttgcg
     1981 ggtcgtgatg tcgacgctcg agccgaccag gcgcagcgct ttaccgctct cgtcccaagt
     2041 ggcttgcccg tggatctcga accaacggta ctcgccagag tcggcgagcg cccgcagctc
     2101 gagcgcctgg aaaggcgcgc gcgattggag atgcttcgag agcgcttcgg cgacccggct
     2161 gcggtcttcg gggtggacca agcgctccag ctccgccaca tcgaaacgag cgcccgcggc
     2221 tggcactgga ccgtctttcg ggcccaatag cgagacttcg ccagtgccga gatcgcagtc
     2281 gaacaggatg gcgttcgacg ctgcgaaggc caacctcaag cgctcttcac tcgcctggag
     2341 cgcactctgc gcccgcacgc gatccgtcac gtcacgggcg atgccgacga gtccgatcac
     2401 cgcgcccgat ggcgagacga tcgcggcgtt ggtagtcgaa agccaggcgg gctcgtcgtc
     2461 gccccggcgc gggtgcggat gctcggcgtt acagcgtgcc tccgtgccca gcagcaccgg
     2521 cttctccgcg agctcgagcg cgtgcgcgac cgggctgccg tcgagcatct ccgagagtcg
     2581 cgcgccgatc acacggctct cgtcggcaac cccgaacata tccaagaagc cgcggtttac
     2641 ccgaacgaac cggtgctcgc gatctttgaa gtagatcgcg tcaggcatgc tgttcatgag
     2701 cccgtgcagc agctgacgct cgtccgctag gtcttgagtg agccggaagt gctcggcggc
     2761 gcgaacgact ttgttggtca aatccggcgc gtcccaaggt ttcgtgacgt acgcgaaaat
     2821 ctgaccttcg ttgatggcgc gcatgatcgc ctggaggtcc gcgtaaccgg tgacgagtag
     2881 acgcgcggcc gagcagcgct cgcgcaatcg agcgagcagc tcgtgaccat tcatgtgcgg
     2941 catccgctcg tcggagagga tcaccgcaag gtcgggctcc tgctcggcga ggcgcaacgc
     3001 gcgcgcaggg tcatctgtag cgacgacgtc gaagaactcg gagagcaagt cttcgagagc
     3061 aacgagcact tggggttcgt cgtcgacaat cagcacgcgc tctctacggg gccgttcgtc
     3121 ctcggcgcca agagaccgca gcgcccccag gggcgagcga aggcgctggg cggattccgt
     3181 aagcccgccg acacgatcac cggtcgattg actgctcttt tgagtcgagt cggcagtgca
     3241 atcgttggcc ggcggtcgaa cggtgtgccc actatggtgt gccatggccc ctttgctctg
     3301 ggacgctgct ctcagcgcgt acaacccttt ggtttcgtac gatgcgcccc gatgcgcgca
     3361 atgatctcgt tacggatctg ccgaaccccg ggttaaggcc gagagagagg acgcgcgcga
     3421 ccgcagcacc tagttggggc aaaaacgcac tcggaccaag gcggctgatg gtgcttttcg
     3481 ccaactcgaa gattcgagtc tcgtacgtac gtgcgccggc gagatggcct gcccccgact
     3541 cggatcctga gggggccgcg aactcggacc cgttcgctgc gcgcgccgat catctactct
     3601 ggcgcggtgc gcatctgcag gcactgaagg ccagtctacg tgccgcagcc ttgcgcgtgc
     3661 aagtggagca cactgtcgat ggcggctacc acccgtgcag tcgtaaatgg cttgttgatg
     3721 agcacgttgg gccccttctc gaagaacgag agagcttctg gcgagtgcac tccaccggac
     3781 atgaagacga aacgcgcggc gagctctcgg cgagcggccg agacgacttt gaagagatcg
     3841 atcccggaca tagtcggcat caccacgtcg caaacgatga cgtcgtagtc ctcccccagg
     3901 aggagctgat acgccgcggt agaggagttc gactcgaagg tcacgtcgta gcggtctcgc
     3961 aaaatgcgct gaatcgtccg cccaaccgcg acttcgtcgt ccacgaccag gatgcgcggg
     4021 acgtgctcac cgtaccctcc ctggctcggg cgcgccccgg gtcgagcttg cacacccgat
     4081 tcgcgccggg ccggcgggcg accgttcgtc ttgcgtacgc cgtctttcga cgattccccg
     4141 tcgcgaagac ggcgtaccag tgacctgagc tccgtcgtcg aggtcgcggc ctgagcgagg
     4201 gcctgggtca cgctgccgag ggcgaccgcg ctcgcctttc cgccgtccat gctcgcaagg
     4261 tctgcgacac ccgcaaggac gctcgaggca aactcgatgt tcgcgacgat cgacgcaatc
     4321 ggcccctcga cgaggcgtga gatctccgcg agcgtgggct ccgagggcgc gaccgaaccg
     4381 ttccaacgct ctgcgtgagc gaaatctgac aggtgatgac cgcgggacaa ttcaaactcc
     4441 tgaagctgac gagagcttag gtttattccg cgctcgcggc tgccatgaaa aaccgagagt
     4501 gcgatcatga aatcgtgatg agtcgcgaag cgagtggata ccgtacacgc gcccgcaaca
     4561 ttcgagctca tcctggcatc gacgcgaaat aaaactgtcg ccgttccgct gccgctcgga
     4621 cgcaatcgag gagactcgga atcggagtag cccagcgtcg atgtcgacga agcggcagac
     4681 aaggcccttc gatgacgctc cctcgccgcg agtcgcacca gtcactgaaa acggtcgtcc
     4741 gtaaactcag ccgcgagacc aagccgaccc accgcggcgt tcgggccgcc acacgtcgat
     4801 ccgagcgctg tcccgataac cacggacatc gggaagcttt gcctcgccga gagcgcgtcc
     4861 ctctcgaacg cgagacgcaa cgatctgcac gttcggaccg cgtcagcccg caatcggcga
     4921 aacgtcgagt ctagaaaaca ctacgcatcg agccgaggaa gcccgagcac gggacctgca
     4981 acgaattggc accgtgtgat gtccgaatgg aggaaccaat ggcaaagctc gtccgtgagc
     5041 tcatgagcaa gccgctcgcg ctgccctcgt ccgcgcccgt cgtagaagcc gcccggcaga
     5101 tgcagaacga agacgtcggc acggtgatcg tcgaggatgg cggcaagcca ttcggcatcg
     5161 tgacggatcg cgacatcgtg gttcgggtcg tcgcggaggg tcgcgatccg gcgcagactg
     5221 ctatttcgga ggtctgtagc aaggaactcg tgacgctgag ccccgacgcg gaactcgact
     5281 tggccgtcca gctcatgcga gatcgggccg tgcgccgctt gctcgtggtg aacggagagc
     5341 accgaaccgt cgggctgatt tcactcggcg atctggcaat cgagcgcgat ccgaactcgg
     5401 tcttgggtca aatcagcgcg acacccccga acagctgacc ctgcccaccg aaagcgctag
     5461 gcgttgggag atagtgaaat gaaggttcga gagctgatga cggtcaacgt cgcgcgggtg
     5521 gcgagtgacg aacccttgag cagcgccgcg cgcttgatgt gggactgtga ttgcggagcc
     5581 gtcccggtca ccgacagcat caatcaccgg gtgatcggca tcctcacgga tcgcgatatt
     5641 tgtatggcga cgtggatccg cgacaagccg ccgagcgaga tccccgttcg tgaggcgatg
     5701 tcgcgcgact tgttctactg cagccccgat cagagcattt cgtccgccga gagcttgatg
     5761 cgcgcgaagc aggtccggcg cattcccgtg ctggacgacg acaagaacct gctcggcatt
     5821 ctctctttgg ccgacatcgc caagcagtcg caacgcgaag gtctgcgcgg agcgagcgca
     5881 ctcgcgcccg aggaagtctc gacgacgctc gcccaaatct gcgaacggcg cgacttcgct
     5941 cgagcggggt agcggcaggg gtccccacgg ctgccgcacc gtcggcgacg tctcgtccag
     6001 actcggtcga agtgcgctgg ggtggggaca tctagccaga cttcccgttt tgcacgtgcg
     6061 ggtgagccga cggcaggggc catcgctgca gggcggtttc ccctcgaacc caggcagtct
     6121 gcgttgctgc cgtcgctcgt gagggaaagt cgcttttcgc ctaaggactc tcgagcgcga
     6181 gtcgtacaat aacatcgatc gcagatattt cgcggcgcgc ccttcgcgcg cgcaagcggc
     6241 cagccgtcgc gccaccggag gttccgcggg ccgggccggg ccgagccgtc ccccaccatg
     6301 attgtcccga cgaaccagta cgtttctttt cgcctcgccg cggagactta cgcaatcgac
     6361 gtctcgcacg cgaaagagat cgtggaagcg ccgcgtatca cggcgttacc acactcccca
     6421 ccttggatac gcggagtgat gaacctacgc ggcagcgtgg ttcccgtcgt cgacctcaaa
     6481 agcaagttcg cgatgggcac gaccgagctc actgcacaca cgtgcgcgct actggtcgaa
     6541 ttcgaagccg aagaaggtcg atttctgatc gccgtgctcg tggacgccgt gcttgcggtc
     6601 ttcgagctgt tggcgaacga gctcgagtcg gcgcctgcgt tcggcgcccg ctattcgcgc
     6661 aaatacttga agggcgtggg ccgccgagaa ggcgtgctgt tcatggtgct cgacgcgaac
     6721 gaggttttcg ccgacgtgga gcccgagctc ggggacgccc ccgcagaaaa cgacgaacga
     6781 gagcccgccc cgcccctctc gggtccgcgc cccgaagacg ctccctccct ctagacgatc
     6841 gacggaacag acacaccatg cagtggttca ataacctaaa gctcgccgta aaactcacca
     6901 gctgcttcat tctactcgcg attttggtcg gcctggttgg caacgtcggc cgctcctcta
     6961 cgaacgagct cggcggacgc gtccagctca tgaaggagaa cgtcatcgag gcaatcgtga
     7021 atctccgcga tgccgagcga cagatgacgc tgtaccgcgg cgacgtgtgg aagctgcttg
     7081 cagacgaggg cgcgccgcac ggcgagctca tacgtgagat cgaagcgcgg cccgcggccg
     7141 tggagcgcgc gctccaaaag tacgactcga ggaccaagtt ccccggagaa gccgagctcg
     7201 tacaggaatt tcgctccaag ttcgcggagc tcaccgcgat cagaaaccgg gccgtgagta
     7261 tggaggcaac ggcgcatcac gacgccgttc gtctgatgcg cgactcgacg cccaccgaga
     7321 aggcggcgcg cgcggcgctc tcgaagctca tcgagctcaa cgtcgagcaa gctcgcgtgc
     7381 agagctcggc ggcggtggag acgagtcatc aggcgagtcg gcagatcctg ctcgcatccg
     7441 tcatcgcggc ggcgctcgcc atcggcctcg gcctactgct ctcgcgttca ctcacgtggg
     7501 ccatcggcga agtcgccaac gcagccgaac gtatcgcggc cggtgaactc gacgtcgaga
     7561 tcgccttgca acaaaaggac gagatcggtc atctcgcgca gactcagcgc cgcatgaccg
     7621 cgcagcttcg ccaaacggtg atgcagatcc aagccgtcgc cga
//
LOCUS       DASTKS010000884         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_365135, whole genome shotgun sequence.
ACCESSION   DASTKS010000884 DASTKS010000000
VERSION     DASTKS010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365135"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1390
                     /gene="fliD"
                     /locus_tag="VFQ35_18405"
     CDS             47..1390
                     /gene="fliD"
                     /locus_tag="VFQ35_18405"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HET9932685.1"
                     /translation="MANVTFSGIASGIDTSSLITAMMNQAKIPMNQLTDQKSDYTSQS
                     KKLTDIKTKLTALQTAVKALDTKNEALGNKVTSSESDVLTATGTGGAAMGAFKLEVTS
                     IAKAERSYSAGFSSSGDAGLVGSGTLSIQVGANDAVDIDITASDTLSTIAQKINSSGA
                     DVTAGLFYDGSSYRLQVTGKKTGTANAITFNETPGLTLGLSAAGAEKQAASDAVVVVD
                     GLAELTVTSSSNTITGAVPGVTLTVLDTGTSTVQVDRDGDGLKTKLNTFIKAYNDVMN
                     SLNSEFAYTGVAKGRDSLSGDGSIQSLQASLRGLVTKAVGNGSSELTTLGAIGVSPQR
                     DGTLSLDETKFNKAVSSDYDGVASLLAGQVDGSGIMGGLVNGLDPYLRTDGILRTKID
                     NLASRNRSIDKQIDSMQTRLDHYEEQLQAKYALLEQTIGGLQSQSSALTSIMSSL"
     gene            1406..1804
                     /gene="fliS"
                     /locus_tag="VFQ35_18410"
     CDS             1406..1804
                     /gene="fliS"
                     /locus_tag="VFQ35_18410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HET9932686.1"
                     /translation="MSFAVAQYRSNQVQTASPARVIVQFYDGALKFVRLAVQGIEARD
                     YAAKGMHLSRAHAIVSELRANLDPTRAPELCAELDRLYVYVLDCITEANIKTDATALA
                     PAVKVLEQLRAAWAQVADEGTGTVRVIGTP"
     gene            1801..2142
                     /locus_tag="VFQ35_18415"
     CDS             1801..2142
                     /locus_tag="VFQ35_18415"
                     /inference="COORDINATES: protein motif:HMM:NF017237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HET9932687.1"
                     /translation="MSSFEALMDKIESVALELDAERPDQLLAALATREALLVQLQGAD
                     TSSLLADARARLKARLHAVLARDTELLHQVTSLRSEIKDALAQLKPARAATRAYRETV
                     TEPVQGIRRIG"
     gene            2169..>3291
                     /locus_tag="VFQ35_18420"
     CDS             2169..>3291
                     /locus_tag="VFQ35_18420"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HET9932688.1"
                     /translation="MQGHRLADAFDGRSSVHGGGALAHDARAADVREHGSSVHRKFTK
                     SSQGEAMNVSQQPNVPPVDRPLASWRPLDATSEAPDIWAVAGGKGGVGKSVVAANLAV
                     AVARRGLRCLLIDADLGGGNQHTLFGVEAPRVTLEAFLQGDEKELSKVATATRFHGLS
                     LVYASSDAVGSANPKHSQKQKFIRHFKRADADVVILDLGAGTSFNTLDLFLSARVQLV
                     VTTPELTAIQNAYGFIKCASMRAKASDALQACAPRLVANACDEEGANRVFRALRSVTS
                     AFLAAEPLALGFVRKDPAMPTSVERGVPVLALSPSSVSSRDLDRIAQMQLEERFTPPA
                     EIAYDNTVARGLNEELRVMERLYHVQTEDLGSEKRQIRTQ"
BASE COUNT          638 a         1087 c         1060 g          506 t
ORIGIN      
        1 cttgccttac tgcttctcgt tcctcttgtt gtgaggagtc gtactcatgg ccaacgtcac
       61 gttcagcggc atcgcctccg gcatcgatac gtcatcgctg atcaccgcga tgatgaatca
      121 ggccaagatc ccgatgaatc agctgacgga ccagaagagc gactacacct cccaatcgaa
      181 gaagctcacg gacatcaaga ccaagctcac cgcgctccag acggccgtca aggcgctcga
      241 caccaagaac gaagcgctcg gcaacaaggt cacctcctcg gagagcgacg tgctcaccgc
      301 gacgggcacg ggcggcgccg caatgggcgc gttcaagctc gaggtcacgt cgatcgccaa
      361 agcagagcgc tcctacagcg ccggcttctc gagcagcggc gacgcgggtc tggtcgggtc
      421 cggaacgctc agcattcagg tcggcgcgaa cgacgcggtc gacatcgaca tcaccgcgag
      481 cgacaccctc tcgacgattg cgcagaagat caactcgtcc ggagcggacg tcaccgctgg
      541 cctcttttac gacgggagca gttaccggct ccaggtgacg ggcaagaaga ccggaacggc
      601 caacgccatc acgttcaacg agaccccagg actcacgctc gggctttccg ccgcgggcgc
      661 cgagaagcag gccgcgagcg acgcggtggt ggtcgtggac ggcctggcgg agctgacggt
      721 cacgagctcg tccaacacga ttaccggcgc ggtccccggc gtgaccctca ccgtgctcga
      781 taccgggacc tcgaccgtgc aggtcgatcg ggacggtgac gggctcaaga ccaagctgaa
      841 caccttcatc aaggcctata acgacgtcat gaactcgctc aacagcgagt tcgcctacac
      901 gggcgtggcg aagggccgag actcgttgag cggcgacggc tccattcagt cactgcaggc
      961 gtccctgcgc gggctggtga cgaaggccgt cggaaacggc tcgagcgagc tgactacgct
     1021 cggcgcgatc ggcgtaagcc cacagcgaga cggtacgctg agcctggatg agaccaagtt
     1081 caacaaggcc gtgtcgtccg actacgacgg agtggcctcg ctgttggccg gacaggtcga
     1141 tggcagcggc atcatgggcg ggctcgtcaa cgggctcgat ccctacctgc gtaccgacgg
     1201 aatcctgcgc accaagatcg acaacctggc gtcgcgcaac cgcagcatcg acaagcagat
     1261 cgacagcatg cagacacgcc tcgatcatta cgaagagcag ctgcaggcca agtacgcgct
     1321 gctcgagcag acgatcggcg gactgcagag ccaaagcagc gccctgacta gcatcatgtc
     1381 ttcgttgtaa gggagagaga cgactatgtc attcgccgtc gcacagtacc gctcgaatca
     1441 ggtccagacc gcctctcccg cgcgcgtcat cgtgcagttc tatgatggcg cactcaagtt
     1501 tgtaaggctc gcggtacagg gcatcgaggc gcgcgactac gcggccaagg gcatgcacct
     1561 gtcgcgcgcc cacgcgatcg tgtcggagct gcgggcgaac ctggacccga cgcgggctcc
     1621 cgagctgtgc gcagagctcg accgactcta cgtgtacgtg ctcgactgca tcaccgaggc
     1681 gaacatcaag accgatgcaa cggcccttgc ccccgcggtg aaggttctcg agcagctgcg
     1741 cgcagcctgg gctcaggtcg ccgatgaagg cacgggcacc gtccgcgtga ttgggacgcc
     1801 atgagctcgt tcgaggcgct catggataag atcgagtcag tcgccctcga gctcgatgcc
     1861 gaacggccgg atcagctgct ggccgccttg gccacgcgtg aggcgttgct cgttcagctc
     1921 cagggcgcgg acacgagcag cctgctcgcg gacgcacgcg ctcggctcaa ggcgcggctg
     1981 cacgcggtgc tagcgcgaga caccgagctg ctgcaccagg tgacgtcgct gcgctccgag
     2041 atcaaggacg cgctcgccca gctcaaaccc gcgcgcgccg ccacgcgcgc gtatcgcgag
     2101 accgtaaccg agcccgtgca aggcatacgg cgcatcggct gaagtggcac atgcgttgcg
     2161 aggcacaagt gcaaggtcat cgactcgcag acgcatttga tggaagatcg agtgtacacg
     2221 ggggtggagc gctcgcgcac gacgcgcgcg cggcggacgt gcgcgagcac ggctcctcgg
     2281 tgcaccgaaa gttcacgaag tcctctcagg gcgaagcgat gaacgtctcc cagcagccga
     2341 acgtgccccc ggtggatcga cctctcgctt cgtggcgccc gctcgacgcg acgagcgaag
     2401 cgcccgacat ctgggcggtc gcaggcggca aaggtggcgt cggcaagagc gtcgtggccg
     2461 cgaatctggc cgtggccgtg gcgcggcgcg ggctgcgctg tctgttgatt gacgcggatc
     2521 tcggcggcgg caatcaacac actctgttcg gggtggaggc gccgcgcgtg acgctggagg
     2581 cgttcctgca aggggacgaa aaggagctca gcaaggtcgc caccgccacg cgctttcatg
     2641 ggctgtcgct ggtgtacgcg agcagcgacg cggtcggctc tgccaacccc aagcactcgc
     2701 aaaagcaaaa gttcatccgc cacttcaagc gcgccgacgc cgacgtcgtc atcttggacc
     2761 tcggcgccgg gaccagcttc aacacgctcg acctgttcct cagcgcgcgc gtgcagctcg
     2821 tggtcaccac gcccgagctc accgccattc aaaacgcgta cggtttcatc aaatgcgcga
     2881 gcatgcgcgc caaggcgagc gacgcactgc aagcgtgcgc gccgcggctg gtcgccaacg
     2941 cctgcgatga agagggcgca aaccgcgtgt ttcgcgcgct ccgcagcgtg acctcggcct
     3001 tcctcgccgc tgagccgctt gcgctcggct tcgtgcgcaa ggatcccgcc atgcccactt
     3061 cggtggagcg aggcgtgccg gtgctcgcgc tgtctccctc gagcgtcagc tcgcgcgatc
     3121 tggatcgcat cgcgcagatg cagctcgagg agcgcttcac gccccctgcc gagatcgcgt
     3181 atgacaacac cgtcgcgcgc gggctcaatg aggagctgcg cgtgatggaa cgcctatatc
     3241 acgtgcagac cgaagacctc ggctcggaga aacgtcagat ccggacgcaa g
//
LOCUS       DASTKS010000885         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_142985, whole genome shotgun sequence.
ACCESSION   DASTKS010000885 DASTKS010000000
VERSION     DASTKS010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142985"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VFQ35_18425"
     CDS             <1..1325
                     /locus_tag="VFQ35_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9932689.1"
                     /translation="GDAAADEKLSIYTSSDGLNFALLSRTGYGGPTNVLRDPSIMKHT
                     DGKYYIAYTLQSWTTSSTSFGIASSTDLKRWTFLVEVPAGVAKARDTWAPEWFVDGDE
                     VHLIVNIDTLGNDSDFRSYDFKAKDSTLTSWDAPVSLGIGPNNIDTFVVKQGATYHAF
                     SKNETTKFIEHATANALHGPWDWIQTGDFAGWGSGKEGIALFKLDDGRWRMFLDCYSG
                     CGFLYADSSDLSKWSKTTTVPGGLSGVVRHGTVLREDDASGGGSGGVSGLGGASGRGG
                     AGQGGTGQGGATSGGTAGARNEAGRSAGGNASGGASGGTTGSAATGGRLSEGSGGTER
                     ASGGGSSSTGTGGATSSAAGGASSAQGGASAASTTGGSPPHPDDNDASSGCAVRPSGV
                     GSVWSTLGALGVVLAARLRRTRRAKGGARPPDARAECDRPNSRRGSQR"
     gene            1322..3205
                     /locus_tag="VFQ35_18430"
     CDS             1322..3205
                     /locus_tag="VFQ35_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9932690.1"
                     /translation="MKATRRILARSRAGNLGHALQRQTFGTSPQVTENGSAKSPRAHK
                     ARLEHRAIKLIGVLAAFAGLACEATTSPNGESQAGATNGGASGGGTTSVVQGGARTNG
                     GTNASGGTTNAAGGADGKGGSATSGGTSTSGGSPSGGNSTSGGAGRNAGGTVGSSGSG
                     GQSGSASGGAPGGSGGRPQVKAESGATLVKVDPNTKRQSFEGWGSSICWWGDRVGGWA
                     EDKRNALVDLIVDAKNGLGYNIFRYNIGGGENPAHTHMGEHRDMPGFEPSKGTWDWNA
                     DARQQAVLKRLLSSGQNIILEAFSNSPPYWMTKSGCASGSSDGSDNLKDDQYDAFADY
                     LTEVVKHYHDELGVTFRTLEPLNEPNANWWKSNGGQEGCHFGPSNQQRIIKAVGAALA
                     SKGLTGTTVSASDENSMDDAYNNVRGFDSTTLGYMSQLNTHSYAGTKRAEVRALATDK
                     KKRLWQSESGPLSVDLANEMEAAIFMAGRIILDLRELQPNAWIDWQLLDTSPTWTSFV
                     FDDKKQSFTPNKRFYMHSGFSRYIRPGATFVSIDNADMVAALSSDEASVTIVVRNGDK
                     SATRKFTFDLTRLPVLPASVEVYRTSSSENLAHLDPMKPGDWSFTATMPASSVTTFVL
                     ALK"
     gene            3411..4892
                     /locus_tag="VFQ35_18435"
     CDS             3411..4892
                     /locus_tag="VFQ35_18435"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HET9932691.1"
                     /translation="MTFVHRAHRGLAQGSVWLGIALSLACSSEGGGGGSGGKSAGSGG
                     STQNGGTASSTGGTATSGGASSGGVGNGTGGAALGGTQNGGGGASGGRSASGGEVSNA
                     GKGGGGAASGGAMSGGASSGGANGGVPNGGGGSGGKATGGSANGGSGGSAGNAFKGTF
                     HLGADITDQEVQTEALRGTLLSIMKSHGFNSVRLRTFVDPRASDGYDKTNGYDDITHT
                     VAFGKQIKDAGMGLFIDFHYSDNWADPGKQCVPVAWQKYTTIGELATALHDYTKDSIT
                     KLIAGGARPDLVQIGNETTPGMLLHRCDSGGQPTGNNPITGSTSNWANLGALLKAGVD
                     AVREVDPTIMVSFHIDRGGDKASETPGSALQFSTSWLTNALKYTSVDAFGESCYQKYQ
                     GDPNSTANTKAGWKSTFTGLASKFPNIKLFAAEYGPMQREVNDVVFELANQQGIGTFN
                     WEPTTQGDWNTGHDLLRRSGSTYTAQPDLALYDQMKIDYASRL"
     gene            complement(4894..5409)
                     /locus_tag="VFQ35_18440"
     CDS             complement(4894..5409)
                     /locus_tag="VFQ35_18440"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HET9932692.1"
                     /translation="MTAMVVLHQDMTPDRLDRFRLAQDSDDDGFAEALHELRTTGKRS
                     HWIWYIFPQLAGLGSSSMARAFGLDGIAEATVYFRDPILRERLLDATAAVSERLDQGM
                     PLTRVMGSEIDSLKLVSSLTLFEAIAAKAGPEHERLAKLAGHVLERAEAQGYPRCSFT
                     SKALELDRQNK"
BASE COUNT         1142 a         1763 c         1896 g          838 t
ORIGIN      
        1 tcggcgacgc ggcggcggac gagaagctca gcatctatac gtcgagcgac gggctgaact
       61 tcgccctcct gtcgcgcacc ggctacggcg gaccgaccaa cgtgcttcgc gatccgagca
      121 tcatgaagca caccgacggc aagtattaca tcgcctacac gctgcaatca tggactacga
      181 gctccacgag ctttggcatc gcgtcgagca cggatctcaa gcgctggacg ttcctcgtcg
      241 aggttcccgc gggcgtggcc aaggcgcgcg acacctgggc accagagtgg ttcgtcgacg
      301 gcgacgaggt gcatctgatc gtcaacatcg acacgctcgg caacgacagc gactttcgtt
      361 cgtacgattt caaggcgaag gactcgacct tgacgtcgtg ggacgcgccg gtgagcctcg
      421 gcatcggtcc gaacaacatc gacacgttcg tggtgaaaca gggcgccacg tatcacgcgt
      481 tttcgaagaa cgagacgacg aagttcatcg agcacgcgac cgcgaacgct ctgcacggac
      541 cctgggattg gattcaaacc ggagacttcg cgggttgggg ctccggtaaa gagggcatcg
      601 ccctgttcaa gctcgatgac ggccgctggc gtatgttcct cgactgttat tccggctgtg
      661 ggtttctcta cgccgacagc tcggatctct cgaaatggtc gaagacgacg acggtacccg
      721 gtgggctttc gggcgtcgtg cgtcacggca cggtgctgcg cgaagacgac gcgagcggcg
      781 gcggaagcgg cggagtcagc gggctcggcg gcgcaagtgg acgaggaggt gctggacagg
      841 gcggcaccgg tcaaggtggc gcgacgagcg gcggcaccgc aggagcccgg aacgaggccg
      901 gacgcagcgc aggcggcaac gcgagcggcg gcgcgagcgg tggtacgacc ggctccgcgg
      961 cgaccggcgg gcggctctcc gaaggtagcg gcggcaccga acgagcgagc ggcggcggaa
     1021 gctcctcgac ggggacgggc ggtgcgacgt cgagcgccgc gggcggcgcg tcttcggcgc
     1081 aaggcggtgc gagtgcggcc tcgacgacgg gcggctctcc tccgcacccg gacgacaacg
     1141 atgcgtcgtc gggctgcgcc gtgcgcccga gcggcgttgg cagcgtgtgg tcgacgctcg
     1201 gagcgctcgg ggtcgtgctc gcggcccgac ttcgccgcac gcgacgcgcg aaaggaggag
     1261 cccgcccgcc cgatgcgcgt gccgaatgcg atcgaccgaa ctcgcgaaga ggttctcagc
     1321 gatgaaagcc acacgcagga tcttggctcg cagcagggcg gggaacttgg gacacgccct
     1381 acagcgccaa acgtttggga catcgccgca ggtgaccgaa aacggctctg caaagagtcc
     1441 ccgcgcccac aaggcaaggc tagagcaccg agccatcaaa ctgataggcg ttctagcggc
     1501 gttcgcgggc ttggcgtgcg aagcgacgac gagcccgaac ggcgaaagcc aagcaggcgc
     1561 aacgaacggt ggcgccagcg gcggcggcac gacctcggta gtccagggcg gtgcccgcac
     1621 gaatggtgga accaacgcga gcggtggaac gacgaatgct gcgggcggcg ctgacggcaa
     1681 gggcggcagc gcaacgagcg gcggcacctc cacgagtggt ggcagcccga gcggcggcaa
     1741 ttcgacgagc ggcggagccg gaaggaatgc gggcggcacc gtcggcagca gcggctccgg
     1801 cggtcagagc ggctccgcga gcggcggcgc accgggcggc agcggcggac gccctcaggt
     1861 caaggccgag tccggcgcaa cgctcgtgaa agtcgatccg aacacgaagc gccaatcctt
     1921 cgagggctgg ggcagcagca tttgttggtg gggtgaccgc gtgggcggct gggcggagga
     1981 caagcgcaat gcgctcgtcg acctgatcgt cgatgcgaag aacgggctcg gttacaacat
     2041 cttccgttac aacatcggcg gaggcgagaa cccggctcat acccatatgg gtgagcaccg
     2101 cgacatgccc ggcttcgaac ccagcaaagg cacctgggac tggaacgccg acgcgcgaca
     2161 gcaagcggtg ctgaaacggc tactctcgtc gggccaaaac atcatcctcg aggccttctc
     2221 gaactcacca ccgtactgga tgaccaagag cggatgtgcc tcgggcagta gtgacggctc
     2281 cgacaacctg aaggacgacc agtacgacgc attcgcggac tacctgaccg aagtggtcaa
     2341 gcactaccac gacgagctcg gcgtcacttt tcgcactctc gagccactga acgaaccgaa
     2401 cgcgaattgg tggaagtcga acggagggca agaaggctgc cacttcggtc cctcgaacca
     2461 gcagcgcatc atcaaggcgg tcggcgcggc cctcgcctcg aaagggctga cgggaacgac
     2521 cgtgagcgcg tccgacgaga acagcatgga cgatgcgtac aacaacgtcc gcggcttcga
     2581 ctccacgacg ctcggctaca tgtcgcagct caacacgcac tcgtatgccg gcacgaagcg
     2641 agcagaggtt cgcgccctcg cgaccgacaa gaagaaacgg ctctggcaat cggagtcggg
     2701 acccctgagc gtcgatctcg cgaacgagat ggaagctgcc atcttcatgg ccggtcgcat
     2761 catcctcgat ttgcgcgagc tgcagccaaa cgcatggatc gactggcaac tcctcgatac
     2821 gagccccacg tggacgagct tcgttttcga cgacaagaaa cagtcgttca cgccgaacaa
     2881 gcgcttctac atgcactcgg ggttcagccg ttacatccgc ccgggcgcga cgttcgtgag
     2941 catcgacaac gccgacatgg tcgccgccct cagcagcgac gaagcatcgg tcacgatcgt
     3001 ggtgcgcaac ggagacaaga gcgcgacgcg caagttcacg ttcgatctaa cccgcctgcc
     3061 ggtgctcccg gcaagcgtcg aggtgtatcg aacgtcgagc agcgagaacc tcgcccacct
     3121 cgacccgatg aagccgggtg actggagctt caccgcaacc atgcccgcgt cttcggtaac
     3181 caccttcgtg ctcgcactca agtaacacgt ctcgattcag gcggttctcg ccaccctcgt
     3241 ggcttggcct gtgggcagcg ttctgcacgc tcggaagcag gcgtgaacgc gcgtttcggg
     3301 gctcagcgag cgacgagccg gagagcgcgc ctcgcacact gggcgatgag aatgagcgcc
     3361 gattcacatt ttgatagtca agtccgccgc tctcggccca ttagaacatc gtgaccttcg
     3421 ttcatcgcgc gcaccgtggt ttggctcagg gctccgtttg gctaggaatc gctctttccc
     3481 tcgcttgctc gagcgaaggc ggcggaggag gcagcggcgg caaatcagct ggctcgggcg
     3541 ggagtacgca aaacggagga accgcgagct cgaccggagg caccgcgacg agcggcggcg
     3601 cgtcttccgg aggggttggc aatggcactg gcggagcggc ccttggcggg acgcagaacg
     3661 gcgggggcgg cgcgagtggc ggacgcagcg cttcgggcgg cgaagtgagc aacgctggca
     3721 aagggggcgg aggggcagcg agcggcgggg cgatgagcgg cggggccagc agcggcggcg
     3781 ctaatggggg tgtgcccaat ggcggcggcg gcagtggagg caaggcgacc ggtggcagcg
     3841 cgaacggcgg cagcggcggg agcgcgggca acgcgttcaa agggaccttt cacctcggcg
     3901 ccgatatcac cgatcaagag gtgcagaccg aggcgctgcg cggcacgctg ctgtcgatca
     3961 tgaagtcgca cggtttcaat tcggtgcggc ttcgaacgtt cgtcgatccg agggcgagcg
     4021 acggttacga caagaccaac ggctacgacg acatcactca cacggtcgcg ttcggcaagc
     4081 agatcaaaga tgcggggatg gggctcttca tcgactttca ctacagcgac aattgggccg
     4141 accccggcaa gcagtgcgtc cccgtcgctt ggcaaaagta cacgaccatc ggagagctcg
     4201 cgaccgcgct ccacgactac accaaagaca gcatcacgaa gctgatcgcc ggcggcgcgc
     4261 gcccggacct cgtgcaaatc ggtaacgaga cgacgccggg gatgctgctt catcgctgcg
     4321 acagcggcgg gcagcccacc gggaacaatc ccatcaccgg gagcacctcg aactgggcca
     4381 acctgggcgc gctgttgaag gcgggcgtcg acgccgtgcg cgaagtggac cccacgatca
     4441 tggtttcgtt ccatatcgat cgcggcggcg acaaggccag cgaaacgccg ggcagcgcgc
     4501 ttcagttcag caccagctgg ctgacgaacg cgctcaagta cacgagcgtc gatgcgttcg
     4561 gagagtcttg ctaccaaaag tatcagggcg atccgaacag caccgccaac accaaagcag
     4621 gttggaagag caccttcacg gggctcgcgt cgaagtttcc gaacatcaag ttgttcgccg
     4681 ccgagtacgg ccccatgcag cgcgaggtca acgacgtcgt gttcgagctc gcgaaccagc
     4741 agggcatcgg cacgttcaac tgggagccca cgactcaagg cgattggaac acggggcacg
     4801 acctgttacg gcgctccggc agcacctaca ccgctcagcc cgacctcgcg ctctacgatc
     4861 agatgaagat cgactacgcg agccgtctct aggtcacttg ttctggcggt cgagctcgag
     4921 cgctttcgac gtgaacgagc agcgcggata gccctgcgcc tcggcgcgtt cgagcacgtg
     4981 accggcaagc tttgcgagcc gctcgtgctc ggggccagcc ttggcggcta tagcttcgaa
     5041 caacgtgagc gaggagacca gcttcagcga gtcgatttcg gagcccatca cgcgggtgag
     5101 cggcatgccc tgatccaggc gctcggagac ggccgcggtc gcgtcgagaa ggcgctctcg
     5161 caagattggg tctcgaaagt agaccgtcgc ctccgcgatg ccgtccaggc cgaacgcgcg
     5221 cgccatactc gaagaaccga gcccggcgag ctggggaaaa atgtaccaaa tccagtgact
     5281 tcgcttaccg gtggttcgga gctcgtgcag cgcttccgcg aagccgtcgt cgtccgaatc
     5341 ttgagcgaga cgaaaccgat cgagacgatc cggagtcatg tcctggtgta gcacaaccat
     5401 cgcggtcatt tggtggtcac atgtccgatg ctcgatgtcc ctaacgaagc acgcggctcg
     5461 cttaccgagc ccgcaaccgt tcggaggcag agccaatgac caaccaaatc gccaagaagt
     5521 tcgtgacagt agccggagtg aggttcgctt acgtcgaccg cggcaaggga gcgcccctcc
     5581 tctttctcca cggcaacccc acgtcctctt acctctggcg aaacatcatc gaaccgctg
//
LOCUS       DASTKS010000886         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_143233, whole genome shotgun sequence.
ACCESSION   DASTKS010000886 DASTKS010000000
VERSION     DASTKS010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143233"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFQ35_18445"
     CDS             complement(<1..541)
                     /locus_tag="VFQ35_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932693.1"
                     /translation="MNQLGRLQRIFLLTFVESFSGVLLQRGLYFYTHDVLGFDRSKNL
                     WLALLSGVAYVIGAVASHPVSKRSGERRLLMVTLGGALSMHLLLMQNPAPWALMTAFP
                     AVALFQGFKWPVIESFLSAGLGPKDLVRTLGRFNVVWAASVPIGLVMAGQLIGASYPG
                     ALFAAAAASNALSIAVAIPL"
     gene            complement(538..1746)
                     /locus_tag="VFQ35_18450"
     CDS             complement(538..1746)
                     /locus_tag="VFQ35_18450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9932694.1"
                     /translation="MVRPDCSSEESAGDPAPDALLTPAFVRVMGAQLAIGFGFSIYFL
                     LPKYLATALHAGPTTIGTVMSTSLAAAVATTPMMGALLDRYGRRIPMALSALVVVVAS
                     LGMLRVREVGALLYALRLLQGVGFALGFNAAAALVADIAPLGRLGQAMGLLGTASLIT
                     NAIAPALFEGVAARWGWAPVFAAASFTGLVTLAVTFSLPRPRANDVTFSAGTATATPT
                     LTTSRINAATLATGAAFGTLISFTQPFALARGAERVSTYLVGYTLGALVVRIALGSLA
                     DRVGRQRVAQWSLFAYGAVALLTSLLEPSLLLPLGILFGAAHGFLYPALAALAADGAS
                     PERRGRTLANFNAAFNSGAGLAMLGGGWVAHAGGYPALFGLVGLITLTSVLSLSASPA
                     KLLLPPESKP"
     gene            complement(1950..>2206)
                     /locus_tag="VFQ35_18455"
     CDS             complement(1950..>2206)
                     /locus_tag="VFQ35_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932695.1"
                     /translation="IAAESSLIESVLSSCVAPQKQRFLVRLVSPTSPGLELRPEQAFL
                     LSRLDHPSGIEELLDISPLPRHETLRHVVQMLRRGVLGFE"
BASE COUNT          458 a          684 c          768 g          296 t
ORIGIN      
        1 ggaggggaat tgcgaccgcg atcgagagcg cgttcgaagc cgccgcggca gcgaacagcg
       61 cgccgggata gctcgcgccg atgagctgtc cggccatgac caagccgatt gggacggatg
      121 ctgcccagac gacgttgaag cgcccgagcg tgcggaccaa gtccttcgga ccgagccccg
      181 cgctgagaaa gctctcgatc accggccatt tgaagccctg gaagagcgcg accgctggaa
      241 atgcggtcat caacgcccac ggggcggggt tctgcatcag caggaggtgc atgctgagcg
      301 ctcctccgag cgtgaccatc agcaggcgtc gctcgcccga gcgtttgctg acggggtggc
      361 tcgcgaccgc gccgatgacg tacgcgacgc cggagaggag cgcgagccag aggttcttgc
      421 tgcggtcgaa gccgagcacg tcgtgcgtgt agaagtagag cccccgctgc aacagcacgc
      481 ccgaaaagct ctccacgaac gtaaggagga agatgcgctg cagtctgcct aactgattca
      541 cggcttcgat tcggggggca ggagcaactt tgcaggagac gcgctgagcg acaacacgga
      601 agtgagcgtg atgagcccga cgaggccgaa caaagccgga tagccaccgg cgtgcgcgac
      661 ccagccaccg ccgagcatcg caagccccgc gccggagttg aacgcggcgt tgaagttcgc
      721 gagagtccgg ccgcgccgct ccgggctggc gccgtcggca gcgagagcgg cgagggcggg
      781 gtacagaaaa ccgtgcgcgg cgccgaacag aatgccgagc ggcagcaaga gcgatggctc
      841 gagtaggctc gtgagcaatg cgaccgcgcc gtacgcgaag agcgaccatt gggcgacgcg
      901 ttgcctgccc actcgatctg ccaagctgcc gagcgcgatg cggaccacca acgctccgag
      961 cgtgtagccg acgagatagg tcgaaacgcg ctcggcgccg cgcgcgaggg cgaagggctg
     1021 cgtgaacgaa atcagcgtgc cgaacgcggc tccggtcgcc aacgtcgccg cattgatgcg
     1081 cgaggtggtg agcgttggcg tcgcggtcgc ggtcccggcg gaaaaagtga cgtcattcgc
     1141 gcgtggacgc ggcaacgaga acgtcacggc gagcgtcacc aacccggtga acgacgcagc
     1201 cgcgaacacg ggcgcccagc cccagcgcgc ggccacgcct tcgaacaggg caggcgcgat
     1261 cgcgttcgtg atgagcgaag ccgttccgag caagcccatg gcttggccga ggcggccgag
     1321 gggcgcgatg tccgcgacca acgcggcggc cgcgttgaag ccgagcgcaa acccgactcc
     1381 ctgtaagagc cgcagtgcat agagcagcgc gcccacttcc cggacgcgca acataccgag
     1441 ggatgctacg acgaccacca aggcagaaag cgccatgggg attcggcgac catatcggtc
     1501 gagcagcgcg cccatcatcg gcgtcgtcgc gacggcggcg gcgagcgagg tggacatcac
     1561 ggtaccaatg gtcgtcggac ccgcgtgcag cgccgtcgcg aggtacttgg gcagcagaaa
     1621 gtagatggaa aagccgaacc cgatggcaag ctgcgcgccc atcacacgca cgaaagccgg
     1681 tgtgagcaac gcgtccgggg cgggatcgcc ggcggattcc tctgaagaac agtcagggcg
     1741 taccatgttg aaagggcttt cccgccgccg agggtccaga aatgcgaaaa ccctctcggc
     1801 tgctgcggag agggtttgaa cgacactcga aattcgacct ggccctcagc cgctgtcctc
     1861 catcgggacg atgcaaatat gcttacggca gagctgcttc atcgaggaac aaggtgtaca
     1921 gcccgagctc ggattcgtca attgccgact cattcgaagc cgagcacacc gcgccggagc
     1981 atctggacca cgtgacggag cgtctcgtgt cgaggcagtg gcgaaatatc gagcagctcc
     2041 tcgatgcctg acggatgatc gaggcgcgac agcaaaaatg cttgctccgg gcggagctcg
     2101 agcccagggc tcgtcggcga gacgagtcgc acgaggaagc gctgcttttg gggggccacg
     2161 caactcgaga gcacgctttc gatgagggag ctctctgccg cgatgg
//
LOCUS       DASTKS010000887         5716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1705695, whole genome shotgun sequence.
ACCESSION   DASTKS010000887 DASTKS010000000
VERSION     DASTKS010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705695"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFQ35_18460"
     CDS             <1..284
                     /locus_tag="VFQ35_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932696.1"
                     /translation="CAMKAGTRWLLVAALLACACENKDKAPESKPAPEPVASAPAAPP
                     PPAAPSQVDLSKAEAPTEADFEADAEQSISAKNLDQELDKLEKEIGDTR"
     gene            1173..1835
                     /locus_tag="VFQ35_18465"
     CDS             1173..1835
                     /locus_tag="VFQ35_18465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9932697.1"
                     /translation="MRVDDSEQSPTRAGLAPSGQRLAAILTKPVHSRTVSSLASEELV
                     PLAAGERSFADIYREHVDFVWRYAATHGIDRASLDDVVQEVFMVVHARLPTFEQRSSL
                     KTWIAGIAVNVVRGYRRRRTARRIGDPLADEHELPAEQASASEALELKRSIELLHGTL
                     ARMSDLQREAFVLCELEQFSQVEVAAMLDVNENTLRTRLRAARAIVYQAVENEREARG
                     LP"
     gene            1832..2560
                     /locus_tag="VFQ35_18470"
     CDS             1832..2560
                     /locus_tag="VFQ35_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932698.1"
                     /translation="MTELDERARESLLRAYQGQGPSESDRERVFAALESAIGAGAVAA
                     PLAVGLKGTLKSILASHPVWLTGAGVGAACAVAAGVWLARLPLQPSQAPAPRPAPSEV
                     VAGPVASSAAPLGAAGPVASVAAPLEVAPSPSAALEPEAKAPPASSESGRSTALRHGD
                     DLAAEAALLHRAHAAYRQGQATETLKLLQEHAGKYPRSQLGVERSTLKVLALCALHRT
                     NDARRIAATLPSLPTALRGSCAEP"
     gene            2487..3578
                     /locus_tag="VFQ35_18475"
     CDS             2487..3578
                     /locus_tag="VFQ35_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932699.1"
                     /translation="MTPGESQRPFRASPRRYAAAALSPSCRPGFGAWLIALGIALLAL
                     VSRPVCAEPEAEPLELLELEWDAPAGCASAPQIREELARIVRARPGRGFPRLIVHARV
                     YRERVGYRAVIEVVREQERTHREFKSADCAALVRAATVSIALSFGDGDGLPEVAPSSS
                     EEATRQEPKPPTPLSSRAAEAVEAARPRESAAPVRRSLAVAPGFAWAPNWLGGNDFGA
                     QATMSVRSTGAVLAWQNRVWLPRSLRATDAVGARFWAASSSVSLALVYAVRPLELEFG
                     IGLQAAVVHGAGFGVASPARAWAPSYSVVPSLSVLLELRRGVRVAVGEELTVALARPK
                     FEIAPVGEVYRVPSLTAITTVSMPFDVSP"
     gene            3649..5316
                     /locus_tag="VFQ35_18480"
     CDS             3649..5316
                     /locus_tag="VFQ35_18480"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HET9932700.1"
                     /translation="MTIRRALFGQIVLGVALGACAQREGEADPSPISQTGGATAGGRG
                     AALGGRNNAAGNPASGGSDSGGRPDKPPASGGAWSSASGGGNAVASGGRTTNTTGGTT
                     SVGGGVSALGGAPAMQGGAASALGGAAAGGRTSSAGGAASGGAGASDPARVVGYFAQW
                     GIYQRAYRVKNIAESGAAAGLTVINYAFGNVVNGACVMVSRAGVGDAFADYQKSFAAE
                     ESVDGAADTWDQKLKGNFNQLKKLKARYPNIKVLISIGGWTWSKGFSDAALTAESRAA
                     VVKSCIDLYIRGDLPMLDGDPAGGPGAAAGVFDGIDIDWEYPAMAGDVGSVYRDEDTK
                     NFTLLLAEFRRQLTAIDPKLLLTIAAPAGKPQSDKIELGAIHQHLDWINLMTYDMHGA
                     WDAKGPTDFHSPLYTSPDEPTSAGAAELSSDATVTRYLNAGVPSSKLTLGIPFYGRGW
                     SGVAGSSHGLYQTTTGPAPGTYEAGIEDYKVLVARKAPGFRDSKAGAYWTFDGNEFWS
                     FDDPENIRLKTEYIRSKRLGGVMVWSLDGDTKDGELMRAVASGMARP"
     gene            complement(5356..>5716)
                     /locus_tag="VFQ35_18485"
     CDS             complement(5356..>5716)
                     /locus_tag="VFQ35_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932701.1"
                     /translation="ALAALALPFALAAVSPFGASLASGAHSPRAVACYTFGAALSLPI
                     LLLVRLLDRNERSPLLPLALTAAATGLIANLVLSLHCASDDPVHLALGHASIGVGWLL
                     AVVLRERFRSRSSASSR"
BASE COUNT          983 a         1834 c         1967 g          932 t
ORIGIN      
        1 ggtgcgccat gaaagcaggc actcgatggc tcctcgtcgc agccctcctc gcctgtgctt
       61 gtgagaacaa ggacaaggcg ccagaatcga agccggcgcc ggagccggtc gccagcgctc
      121 cggccgcgcc accgccacca gccgcgccgt cgcaagtcga cctttccaaa gccgaggctc
      181 caaccgaggc cgacttcgaa gccgacgcgg agcagtccat ttcggcgaag aatctggatc
      241 aagagctcga caagctcgaa aaagagatcg gcgacacccg ctaactaaaa cgaatcctac
      301 aatgtgggtg agggtgcggt cgagttgtcg cgcctcaccc actgattcgc gcacgcaccg
      361 gcacggtaga ggcggatgcg atcacggaca gctcgtagag aaggcgccgt caggaaggca
      421 gtgttctgcc cggcaacatg cgttgctgca ggcggcgccg ggggagcagt cgagttcgca
      481 cgaagtcccc gcgcagtcga gggaggtgcc gcagctatcc aaccccatgc agtagatggc
      541 ggcgtcgtcg ccacgaaggc gtatcggtcc cgcgcacgct ccgtctccgc acgagaggtg
      601 cgtcgtcgcg gccgcgctgt cgatcgaacc tttgcagctg tcgcggcccg cgcaattgac
      661 tgcacaggtc gaagcggtac tgcagtcgac cgcaccgcat gcgcgcgctc caaggcaatc
      721 gatttggcaa ggcgcgccgg ctgcacaagt tacgccgccc gtacaagcat cgtcgccgca
      781 aatgacatgg caggcgacgc cgctcggaca gtcgacttta gttgcgcact gtccagcagc
      841 gcaagtgagg acgcatccgg cgccatcgca tacggggctg gttcctccgc tgccagagct
      901 tccgccgctt cccgcgctga gcgaagcgcc tccagtgcca gtgcctcctt gcggagagcc
      961 gccgcttgct ccactggcca ccgagccccc agtcgctcga gacgtggagt ccacgtccgt
     1021 tccgagcgag taggggttgc gcaaacacgc gctgagggcc cacgcgacac acgccgcgct
     1081 caatttcgtg cggctgccgc ttcgatgtcg ctctggtcgg ggcaggcggc tcaccatgtt
     1141 cgagaacgag tatgccaccg ctcggatgcg cgatgagggt cgacgattcg gagcagtcgc
     1201 cgacccgcgc tgggctggct ccgagtgggc agcggctcgc cgccatcttg acgaagccgg
     1261 tccattcccg caccgtttcg agcttggcgt cggaagaact cgttccgctg gctgcggggg
     1321 agcggagctt cgcggatatt taccgtgaac acgtggactt cgtgtggcgc tacgcggcaa
     1381 cccacgggat cgaccgagcc tcgctcgacg atgtcgtgca ggaggtgttc atggtcgtcc
     1441 atgctcgcct tccgaccttc gagcagcgat cgtcgctgaa gacgtggatc gcgggcatcg
     1501 cggtgaatgt ggtgcgcggc taccggcggc gccgcacggc gcgtcgcatt ggtgatccgc
     1561 tcgctgacga gcacgagttg cccgccgagc aggcatccgc gtccgaggct ctggagctca
     1621 agcgatcgat cgaattgctt cacggcacgc tggctcgcat gagcgatctt cagcgtgaag
     1681 ccttcgtcct gtgcgagctc gagcagttct cacaggtcga agtcgccgcc atgctcgacg
     1741 tgaacgaaaa tactctgcgt accaggctgc gagcggcccg agccatcgtc tatcaagcgg
     1801 tggagaacga acgcgaggcg aggggactgc catgaccgag ctcgacgaac gagcgcgcga
     1861 gagcttgctt cgcgcttacc agggccaggg accgagcgaa tcggatcgcg aacgggtttt
     1921 cgccgcgctc gaaagcgcaa tcggcgcggg cgccgtcgcg gctcctcttg ctgtcggtct
     1981 aaagggcacg ttgaagtcga ttctcgcgtc gcacccggtt tggttgaccg gcgccggtgt
     2041 cggtgcggca tgcgcggtgg cggccggcgt gtggctcgcg cggctgccgt tgcagccaag
     2101 ccaggctccc gcgccgcgtc cggcgccgtc cgaagtggtt gcgggtccag tcgcctcgag
     2161 tgcggccccg ctcggcgccg caggcccggt cgcttccgtc gccgctccac tcgaagtggc
     2221 gccatcaccc tcggctgcgc tcgagcccga ggcgaaagct ccgccggcga gcagcgaaag
     2281 cggacggagc accgcacttc gacacggcga cgacctcgcg gcagaggccg cgctgctgca
     2341 tcgcgctcac gccgcctatc gacaggggca agcgaccgag accttgaagc ttctccaaga
     2401 gcacgcgggg aagtatccgc gctcgcagtt gggcgttgag cgcagcaccc tgaaggttct
     2461 agcgttgtgt gcgctccacc gcaccaatga cgcccggcga atcgcagcga cccttccgag
     2521 cctccccacg gcgctacgcg gcagctgcgc tgagccctag ttgccgcccg gggtttggtg
     2581 cgtggctcat cgcgctcggc atcgcgctgc tcgcgctcgt gtcgcgcccg gtgtgcgcgg
     2641 agcccgaagc ggagcctttg gaacttctcg agctcgaatg ggacgccccc gccggctgcg
     2701 catccgctcc gcaaatccgc gaagagctcg cgcggattgt tcgagcgcgc ccggggcgtg
     2761 gctttccgcg gttgattgtc catgcgcgcg tgtatcgaga gcgcgtcggc tatcgtgccg
     2821 tgatcgaggt ggtgcgcgag caggaacgca cgcaccggga gttcaagtcg gcggattgcg
     2881 ccgcgctcgt tcgcgctgcg accgtgagca tcgctctctc gttcggcgat ggcgacggcc
     2941 tccccgaggt cgcgccgagc tcgtcggaag aagcgactcg gcaagagccc aaacccccga
     3001 cgccactttc gagtcgcgcc gccgaagccg tggaggccgc tcgcccgcgc gaaagcgccg
     3061 cgcccgttcg ccgatccttg gcggtcgcgc cgggctttgc gtgggccccg aattggctcg
     3121 ggggaaacga ctttggcgcg caagcgacga tgtcggtgcg ctcgacgggc gccgtcctcg
     3181 cttggcaaaa cagagtgtgg ctgccgcgtt cgctgcgcgc gacggacgcg gtgggggcac
     3241 ggttttgggc agcctcatca tctgtgtcgc tcgcgctcgt ttacgccgtt cggccgctcg
     3301 agctcgagtt cggaatcggc ctgcaggcgg ccgtggtgca cggcgctggc ttcggagtcg
     3361 cgtctccggc gcgcgcatgg gcaccgagct actcggtggt tccgtcgctc tcggtgttgc
     3421 tcgaactgcg ccgaggagtc cgcgtcgctg tcggcgagga gctcacggtg gcgctcgcgc
     3481 ggccgaagtt cgaaatcgcc cccgttggcg aggtgtatcg agtcccgtca cttaccgcaa
     3541 tcaccactgt aagcatgccg ttcgatgtga gcccgtgacc aacggattcg tcggggcgcg
     3601 gcacggtacg gagtgacgag gcgcccggtc gctctcgtcg ctcctaccat gacgattcga
     3661 cgagcactct ttggacaaat cgttctcggc gtcgcactgg gcgcgtgcgc tcagcgagaa
     3721 ggggaagctg acccgagccc aatctcccaa actggtgggg cgaccgcggg cggaagaggc
     3781 gcagctctgg gtgggcgcaa caacgccgcg gggaaccccg cgagcggagg cagcgacagc
     3841 ggcgggaggc ccgacaagcc gccggcttcc ggcggcgctt ggagttccgc gagcggaggg
     3901 ggcaatgcgg tcgcctccgg cggacggacg acgaacacta ccggcggtac cacgtcggtt
     3961 ggcggcggcg tttcagcgtt gggcggcgct ccggcgatgc aaggcggggc cgcttcagcg
     4021 ttgggcgggg ccgcggcggg cgggcgcacg agctcagcgg gcggcgcggc atcgggaggt
     4081 gctggcgctt cggatccggc tcgcgtggtc ggctacttcg cgcagtgggg gatctatcag
     4141 cgggcatacc gtgtcaagaa catcgccgag agcggcgctg ctgcaggcct caccgtcatc
     4201 aactacgcgt tcgggaacgt cgtgaatggc gcgtgcgtca tggtgagtcg cgcaggcgta
     4261 ggcgacgcgt tcgccgacta ccaaaagtcc ttcgcggctg aggaaagcgt cgacggagcc
     4321 gccgacacat gggaccaaaa gctcaaaggt aacttcaatc agttgaagaa gctcaaggcc
     4381 cgatacccga acatcaaagt gctcatctcg atcggcggtt ggacttggtc gaagggcttt
     4441 tcggatgcgg cgctgacagc agaatcgcgc gctgccgtcg tgaagtcgtg catcgatctg
     4501 tacattcgcg gcgatctacc gatgttggac ggcgacccgg cgggaggccc tggcgccgct
     4561 gccggcgtgt tcgacggaat cgacatcgac tgggagtatc cggcgatggc gggcgatgtc
     4621 gggagcgttt accgcgacga ggacaccaaa aacttcacgc tgctgctcgc cgagttcagg
     4681 cgtcagctga cggcgattga tccgaagctc ttgctcacca tcgcggcacc cgctggcaaa
     4741 ccccagtccg acaagatcga gctcggcgcc atccaccagc acctcgattg gatcaatctg
     4801 atgacgtacg acatgcacgg ggcgtgggac gcgaaggggc caaccgactt tcactcgccc
     4861 ttgtacacga gcccagacga gccaacttcg gctggtgctg ccgagctttc ctctgacgcc
     4921 accgtgaccc gctatttgaa tgcgggcgtg ccgtcgtcga agctcacgct tggaatcccc
     4981 ttctatgggc gtggctggag cggcgttgcc ggctcgagcc atggcctcta tcagacgacg
     5041 actgggccgg cgccgggcac ttacgaggcc ggaatcgagg actacaaggt gctcgtcgcg
     5101 aggaaagcgc cagggtttcg cgattcgaaa gcaggcgcgt attggacctt cgacggcaac
     5161 gagttctgga gcttcgacga cccggaaaat atccgattga agaccgagta catccgctcg
     5221 aagcgcctcg gcggcgtgat ggtctggtcg ctggacggcg acacgaaaga cggagagctc
     5281 atgcgcgcgg tcgcgagtgg catggcgcgc ccgtaagtta tcaaggcttt ggcgtgtcgc
     5341 agtgctcacc aggcctcagc gagaggaagc agacgagcga cttctgaagc gctcgcgcag
     5401 cacgacggcc agcaaccaac cgacgccgat gctcgcatga ccgagcgcta ggtgaaccgg
     5461 gtcgtcgctc gcgcaatgca gtgaaagaac caggttcgcg atgaggccgg tggccgcggc
     5521 cgtgagtgcg agcgggagaa gaggagatcg ctcgttgcga tcgagcagcc tcacgagcag
     5581 cagaatgggc aatgagagag cggcaccgaa agtgtaacag gccaccgcgc gcggcgaatg
     5641 agctccgctc gccaacgaag ctccgaatgg gctcaccgcg gcgagggcga atggtagggc
     5701 cagggcggcg agggcc
//
LOCUS       DASTKS010000888         5572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1259953, whole genome shotgun sequence.
ACCESSION   DASTKS010000888 DASTKS010000000
VERSION     DASTKS010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259953"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2524)
                     /locus_tag="VFQ35_18490"
     CDS             complement(<1..2524)
                     /locus_tag="VFQ35_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9932702.1"
                     /translation="MAWRRGAKLGVCVLASCLSVWTAAKSARAQTHGAERIETGPAHA
                     VVTPPKLERFVEAAYPAEAQARGLEADVVLALEIDENGAVTNANVAESAGHGFDEAAR
                     AAALNFTFVPANRDGRPVKSRILYRYSFHFRPPEPAEQKPALALLRARVSIAGSQTPV
                     AGARVRVLRGAKLVEELVTNSDGAFATKELEPGDYSIAVHSEGFDDYSATEHLDAGEE
                     LSLRYGLQPTAEPAELTVTVHGARPTREVTRRTVTRRELSRVPGTSGDALRAIQNLPG
                     VARPPALSGVLVVRGNADQTTPVMVDGLWLPNVYHFGGLSSVIPTEMLDEINFYPGNF
                     SVRFGRALAGVVDAHLRETRGGDRYHGLLQVDLIDARGLLEGPVPFVKGWNFIGGLRR
                     SHIDAWLAPLLKNRDTYITAAPVYYDYQFILDNHPTPDSYLRIGLIGFDDRFRLVNQT
                     GATGGQLDTVNASWGVGTIYQSKLSERTRLNITMSAARNHVAFSLSTLSANTTALGLI
                     GRGELEWKMWKNATLRSGYELLLAPYNVKGQLPEPTSNNAPSTGSFVTAPPQKFDRSA
                     VFLVPALFTELDAHPNKRTQVVTGVRVDYTRDTGRLDVAPRITARYDLVPGFPKTTLK
                     GGTGLFFQAPGLVELVLKDDQTSLRSQRAFQNSLGIEQELTDRVKLSAEGFLNLLDNL
                     VSRGLDDRGVVRYNNYGKGRIYGGEFMLRYEADERFFGWLSYTLSRSERTWVPGEPSH
                     LFYLDQTHILTALGSYTLGRGWEIGMRFRYVSGNLYTPCPRGIYSSNDTGYLCVSGPL
                     FSERLPPFHQLDIRVDKKFKFKEWALSAYLDLINAYNRKNPDF"
     gene            complement(2808..3992)
                     /locus_tag="VFQ35_18495"
     CDS             complement(2808..3992)
                     /locus_tag="VFQ35_18495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET9932703.1"
                     /translation="MKKLSDYVVFNDRNLARRYQNSPIPMATLYEAYFDGAIDIPGDI
                     YELLDQRNNFVKYTLTPQHFKWAVTNFVPEVTVHTKSQDKRIVREHYDRGNDFFGWFL
                     GERMVYTSGFFNRKDETLEEAQDNKMNLVCQKLRLKPGERLLDIGCGWGTLARHAAKY
                     YGVDATGVTISENQTEFGNRRIKEWGLQDRARILCCDYRDIPYQKFDKIVSLEMVEHV
                     GVKNLKKYFSWVQELLKDDGLFLLQWTGLRRQLAGETLIWGLFMNKYIFPGADASLPP
                     AGMIKPAEEVGWESFSMENVSQHYGWTIRLWHDNWISNRDAIVAAYGERWYRIWHLFL
                     AWSTKIADQGNASCYQVVFNKNLDGFDRTQHILDRGIVHGVRASSLSPESGVPGDAFA
                     AE"
     gene            complement(4302..5000)
                     /locus_tag="VFQ35_18500"
     CDS             complement(4302..5000)
                     /locus_tag="VFQ35_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932704.1"
                     /translation="MDDRSSPVADMLESVSSALAARRARLVERVQRIELALESYRRAV
                     ESQIVREFAKEHAFKLPAPPALSPERAAELLKALVDALPEQAARTDAESPTVSTSAPA
                     PPPTEVEAAPLSTHPALLARVKHARIVVIGALSGRDRASTVPPELAEHVEWIDTERDG
                     AHAIGNLPQRVRQGRVAAVVILDRAVQHRHTEPVVAQAREQGVPIAFAGQGGKASLAR
                     ALAQIEASLAQRTE"
     gene            5214..>5572
                     /locus_tag="VFQ35_18505"
     CDS             5214..>5572
                     /locus_tag="VFQ35_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932705.1"
                     /translation="MERETRRPSIVVIDNSELVLAVTRGELESAGYRVVTHPRTAGAV
                     ALILQEKPDLVLLEVSMGGDSVAKLFGKTQPNGSTMVVLYSSLSVDALAAKVAACGAH
                     GYIRKTPDGVDFLRQVNG"
BASE COUNT          940 a         1754 c         1857 g         1021 t
ORIGIN      
        1 tgaagtcggg gtttttgcgg ttgtaggcgt tgatgaggtc gaggtaggcg ctgagcgccc
       61 actctttgaa cttgaacttt ttgtcgacgc ggatatcgag ctggtgaaat ggcggcagcc
      121 gctcgctgaa gagcggtccg ctcacgcaca ggtacccggt gtcgttcgag ctgtagatgc
      181 cgcgcggaca cggcgtgtac aggttgcccg agacgtagcg gaagcgcatg ccgatttccc
      241 agccgcggcc cagggtgtag ctcccgagcg cggtcaggat gtgcgtctga tcgaggtaaa
      301 agaggtgcga cggctcgccc ggaacccagg tgcgctccga gcgcgatagc gtgtacgaaa
      361 gccacccgaa gaagcgctcg tcggcctcgt agcgcagcat gaactcgccg ccgtagatgc
      421 ggcccttgcc gtagttgttg tagcgaacga cgccgcgatc atcgagccca cgcgagacca
      481 agttgtcgag caggttcaag aagccttccg cggagagctt caccctgtcc gtcagctctt
      541 gctcgatgcc gagcgagttt tgaaaagcgc gctgcgagcg aagcgaagtc tggtcgtctt
      601 tcagcaccaa ctcgaccagc cccggcgctt gaaaaaatag gcctgtgccg cctttcagcg
      661 tcgtcttcgg aaagccgggt acgaggtcgt agcgcgcggt gatgcgtggg gcgacgtcga
      721 gtctccccgt gtcgcgcgtg tagtcgacgc gcacgcccgt caccacttgg gtccgcttgt
      781 tcgggtgggc gtcgagctcc gtgaacaggg cggggacgag aaacaccgcg ctccgatcga
      841 acttttgcgg gggcgcggtg acgaaagacc cggtgctcgg cgcgttgttg ctcgtcggtt
      901 cgggtagctg ccccttcacg ttgtacggcg cgagcaagag ctcgtagccg ctgcgcaggg
      961 tcgcgttctt ccacatcttc cactcgagct cgccgcggcc aatcaagccg agcgcagtgg
     1021 tgttggcgga aagcgtgctg agcgagaacg cgacgtggtt gcgcgccgcg ctcatcgtga
     1081 tgttcaggcg tgttcgctcg gagagcttgc tctgatagat ggtgccaacg ccccacgagg
     1141 cgttgaccgt atcgagctga ccaccggtcg cgccggtctg attcacgagg cggaagcgat
     1201 cgtcgaagcc gatgaggccg atgcggagat agctgtcggg ggtggggtgg ttgtcgagga
     1261 tgaactggta gtcgtagtag accggcgcgg ccgtgatgta cgtgtcgcgg ttcttcaaga
     1321 gcggcgcgag ccacgcatcg atgtgactcc gacgaagccc gccgatgaag ttccagcctt
     1381 ttacgaacgg aaccgggcct tccagcagcc cgcgcgcgtc gatgagatcg acttgcaaaa
     1441 gcccgtgata gcggtcgccg ccgcgcgttt cgcgcaagtg ggcgtcgacg acacccgcga
     1501 gcgcccgccc gaagcgaacg ctgaagttgc cggggtagaa gttgatctcg tccagcattt
     1561 cggtcggaat cacgctcgag agcccgccga agtgatagac gttcgggagc cacagcccgt
     1621 ccaccatcac cggtgtggtc tggtctgcat tgccgcgcac cacgagcacg cccgagaggg
     1681 ctgggggccg cgccaccccg ggaaggtttt ggatggcgcg cagcgcgtcg ccggacgtgc
     1741 caggtacgcg cgagagctcc cggcgcgtca ccgtgcgccg cgtgacctcg cgggtcggac
     1801 gagcgccatg cacggtgacg gtgagctcgg caggctcggc cgttggttga aggccgtagc
     1861 ggagcgagag ctcttcgcca gcgtcgaggt gctcggtcgc cgagtaatcg tcgaagccct
     1921 cggagtgcac cgctatcgag tagtcccccg gttcgagctc tttcgtcgcg aacgcgccgt
     1981 cgctgttcgt aacgagctcc tctaccaatt tggcgccgcg cagcacgcgc acccgagcac
     2041 cggccacggg cgtctggctg cccgcgatgg agacgcgcgc gcgaaggaga gcgagcgccg
     2101 gcttctgttc cgcgggttca ggcggacgaa agtggaaaga gtagcgatag agaattcgcg
     2161 atttgacggg acggccgtcg cggttcgcgg gaacgaaggt gaagttcagc gccgcggcgc
     2221 gcgccgcttc gtcgaagccg tgacccgccg actccgccac gttcgcgttc gtgacggcgc
     2281 cgttctcgtc gatttcgagc gcgaggacga cgtcggcttc gagccctcga gcttgcgctt
     2341 ccgctgggta cgcggcctcg acgaagcgct cgagcttcgg cggcgtcact actgcgtgcg
     2401 cgggcccggt ttcgatccgt tccgctccgt gagtttgcgc tcgcgcgctc ttcgcagcag
     2461 tccacaccga caagcagctc gcaagcacgc acaccccgag cttcgcacca cgtctccaag
     2521 ccattcgaca gcgctcccac cgacgcggac cgaggcggcc ccacgccgtt cgcccgccga
     2581 cgattacgcg tattgagcga ctcagtaaag ctttttgcca ggcttccctg cgagccctgc
     2641 ccagcgagac gcgctcgcag taacagaccg ccgcattgtc gctctcgtct cgagcgccgc
     2701 tcgatttcga actccggtgg atgagtcgaa cgctcgcccc gcgctggcat ccaccggcac
     2761 cgtcgcgagc ccgcgcatcg gcagggctcg cgacagccgg ttacggatca ctcggccgcg
     2821 aaggcgtcgc cggggactcc gctctccgga gagaggctcg acgcgcgcac accgtgaacg
     2881 atcccgcgat cgaggatgtg ctgtgtccga tcgaagccgt ccaagttctt gttgaagacg
     2941 acctgatagc aagaggcgtt gccctgatcg gcgatcttgg tcgaccacgc gaggaacaag
     3001 tgccaaatgc ggtaccagcg ctcgccgtaa gcggccacga tggcgtcgcg gttcgagatc
     3061 cagttgtcat gccacaagcg gatcgtccaa ccgtagtgtt ggctgacgtt ctccatcgag
     3121 aagctctccc agccgacctc ttccgcaggc ttgatcatgc cggccggagg caagctcgcg
     3181 tcagcgccgg ggaagatgta cttgttcatg aagaggcccc aaatgagcgt ctcgcccgcg
     3241 agctggcggc gcaagcccgt ccactgcagc aggaacaagc cgtcgtcctt caggagctcc
     3301 tgaacccagc tgaagtattt cttcaggttc ttcacgccca cgtgctcgac catctcgagc
     3361 gagacgatct tgtcgaactt ctggtacggg atgtcgcggt agtcgcagca gagaatgcgc
     3421 gcgcgatcct gaaggcccca ctccttgatg cggcggttgc cgaactcggt ctggttctcg
     3481 gagatcgtga cgcccgtcgc gtctacgccg tagtacttgg ccgcgtggcg tgcgagggtg
     3541 ccccagccgc agccgatgtc gagcaaccgc tcgcccggct tgagacggag cttctggcac
     3601 accaggttca tcttgttgtc ctgggcctct tcgagcgtct cgtctttgcg gttgaagaag
     3661 ccgctcgtgt agaccatgcg ctcgccgagg aaccagccga agaagtcgtt gccgcgatcg
     3721 tagtgctcgc ggacgatgcg cttgtcctgc gacttcgtgt ggaccgtgac ttcggggacg
     3781 aagttcgtga ccgcccactt gaagtgctga ggcgtgaggg tgtacttcac gaagttgttg
     3841 cgctgatcga gcaactcgta gatgtcgccg gggatatcga tcgcgccatc gaagtacgcc
     3901 tcgtacagag tggccatcgg gatgggagag ttctgataac gacgcgcgag gttgcggtcg
     3961 ttgaacacca cgtagtcact cagtttcttc atcggtgtcg ttccttggtt ttcgggggcc
     4021 tcgtgtgcgc gtcgagtacg acccggcgct agccctctga gccgcgacac ctaagtggtt
     4081 cggagcctga cgtcaaaagt cgtatgacgt ttcaggtcgg cccgtaggcc cgattcggat
     4141 tgccgcgcgt cctttcggct tgaaatctgc ctgcttgaga gatgaggccc cccgaagggc
     4201 tcgcatctcg cgcgctcagg ttcgtcgcgg gctcactgcg ccgcttcggc agggacgccc
     4261 cggtcgcagc gtcggcccgc gaccaggcgt gcaggtcggt ttcattccgt gcgctgggcg
     4321 agcgacgcct cgatctgagc gagcgctcgg gcgagcgacg ctttgccgcc ctgtccggcg
     4381 aacgcgatgg gcacgccttg ctcgcgcgct tgggcaacga cgggttcggt gtgtcgatgc
     4441 tgcacggctc gatcgaggat gaccaccgcc gcgacacggc cctggcgcac gcgctgtggc
     4501 aaattcccaa tcgcgtgcgc accgtcgcgt tcggtgtcga tccattcgac gtgctctgcg
     4561 agttcgggag ggacggtgga cgcgcgatcg cggccagaca gcgcgccgat gacgactatg
     4621 cgcgcgtgct tcacacgggc gagtaacgcc gggtgagtcg agagcggcgc ggcttcgact
     4681 tcggttggcg gaggcgccgg ggcgctcgtg ctcaccgtgg gtgactcggc gtcggttcgc
     4741 gctgcctgct cgggcaatgc atcgaccaac gccttcaaga gctccgcggc gcgctcgggt
     4801 gagagcgcgg gcggcgccgg caacttgaaa gcgtgctcct tcgcgaactc ccgtacaatc
     4861 tgcgactcta cggcgcgccg gtaactttcg agagcaagct cgatgcgctg cacgcgctcg
     4921 accaaccgcg cacggcgcgc ggcgagcgcg ctcgagacac tctccaacat gtccgcgacg
     4981 ggagacgatc gatcgtccat gttcgccggt tctaactcgt ctccgcggca gcgcgagcct
     5041 tcgcgcaaaa atggcgctcg acgcgcgctt cccgctctcg gaaaacgacc gcgcgcgccg
     5101 acacttaagg cccaaaatga gtcatccgcg tcgcttgacc gtgccggccc gagggatact
     5161 ctgaagcaac catgcgctcg ccaaaagggg ctggcgctcg ggagtcgggg ctcatggaac
     5221 gcgaaactcg tcgacccagc atcgtcgtca tcgacaacag cgagctcgtc ctcgcggtga
     5281 cgcgtggaga gttggagagc gcgggttatc gcgtggtgac gcacccgcgc acggctgggg
     5341 cggtcgcgct aatccttcaa gagaagcccg atctcgtcct gctcgaggtg agcatgggtg
     5401 gagactcggt ggccaagctc ttcggcaaga cgcaacccaa cgggagcacg atggtagttc
     5461 tctactcgtc gctctcggtt gacgcgctcg cggcgaaggt cgcggcgtgc ggtgcccacg
     5521 gttacatccg caagaccccc gacggcgtgg acttccttcg ccaagtcaat gg
//
LOCUS       DASTKS010000889         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1035528, whole genome shotgun sequence.
ACCESSION   DASTKS010000889 DASTKS010000000
VERSION     DASTKS010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035528"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="meaB"
                     /locus_tag="VFQ35_18510"
     CDS             complement(<1..736)
                     /gene="meaB"
                     /locus_tag="VFQ35_18510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9932706.1"
                     /translation="MTSVTQLAAGVARGETAALARACRLVDERVGEYRALLHELFPLS
                     GRARSIGITGAPGVGKSTLTDALVAALRERGERVGVIAIDPSSPFTGGALLGDRIRMQ
                     RHALDGEVFVRSLGSRGAHGGLSRSAADTLRLIEAWGATTVLLETVGVGQAEYDFLGV
                     VESAVLVVMPGAGDDVQANKAGIIEAADLIVLNKADRPGADAAESELRLALALGQTAI
                     VPSMAHGAGVPLVSSADGWSKPVLRTV"
     gene            complement(762..2126)
                     /gene="accC"
                     /locus_tag="VFQ35_18515"
     CDS             complement(762..2126)
                     /gene="accC"
                     /locus_tag="VFQ35_18515"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9932707.1"
                     /translation="MFRKVLIANRGEIAIRIIRACRELGIQTVAVHSEADNGALHVRF
                     ADEAVCIGPALASKSYLHIPAIISAAEITAADAIHPGYGFLSENAEFAQVVQRCGLHF
                     IGPSPEAMLRWGDKIRAREIAQKFGLPLLPGSEALANVEHAESEAKRVGFPIMMKARG
                     GGGGRGMRIVRELSEVRRAFESCTSEAASAFKNPELYLERFVERPKHIEFQALADNHG
                     QVWTLGERECSLQRKNQKLIEEASSPSMNDALRAEMGERIRHAIRETGYNSLGTLEFL
                     MDEHGKLYFMEMNTRVQVEHPVTEMATGVDLVVEQIRAAAGERLELPDTRPWKFRGHA
                     IECRIMAEDPVTFAPWPGKILEYHPPGGAGVRVDSGVFGGWTVPSQYDSLLAKVIVHA
                     PTREQAIRRMRRALDEYIISGIRTNIPLQKRLLDLPEVRDGSMTTRTVENLMAADALA
                     RARN"
     gene            complement(2132..2584)
                     /gene="accB"
                     /locus_tag="VFQ35_18520"
     CDS             complement(2132..2584)
                     /gene="accB"
                     /locus_tag="VFQ35_18520"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET9932708.1"
                     /translation="MQIALEQLEALLKTLEEKKVGEFEYEDEKIRLKIGFGRAPAAAA
                     QAHFVHAAPAAAPAPAAAAAVEVNDASIAFITSPFVGTFYRAPSPEAENFVEVGAQVK
                     KGQPLCIIEAMKLMNEIEAEVGGTIVEILVENGKSVEFGQKLFKIKKS"
     gene            complement(2820..>3284)
                     /gene="aroQ"
                     /locus_tag="VFQ35_18525"
     CDS             complement(2820..>3284)
                     /gene="aroQ"
                     /locus_tag="VFQ35_18525"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9932709.1"
                     /translation="RTAAAKTTLNVLVLSGPNLDRLGRREPEIYGRTTLPQIHDRLEA
                     VAAELGVRVECRQSNHEGLLIDWIGAASDDGFGAILINPGAYTHTSYALHDAIKGAGV
                     PTVELHLSNPDAREAFRRHSCVAPACLGRIAGFGAASYEVALRAVVQHLGAV"
BASE COUNT          521 a         1082 c         1103 g          578 t
ORIGIN      
        1 cgaccgtgcg cagcaccggc ttcgaccagc cgtcggcgct gcttacgagc ggcacgccgg
       61 cgccgtgagc catgctcgga acgatcgcgg tttggcccaa cgcgagcgcg aggcggagct
      121 cgctttcggc cgcgtcggcg ccggggcggt cggccttgtt gagcacgatg agatcggcgg
      181 cctcgatgat gccggccttg ttggcttgca cgtcgtcgcc ggcgccgggc atcaccacga
      241 gcactgcgct ctcgacgaca ccgagaaagt cgtactccgc ttggcccacg cccacggttt
      301 cgagcagcac cgtcgtcgcg ccccaagctt cgatgagccg cagcgtgtcg gctgccgagc
      361 gcgagaggcc accgtgcgca ccgcggctgc cgagcgagcg cacgaagacc tcgccgtcga
      421 gcgcgtggcg ctgcatgcgg atgcgatcgc cgagcagagc gccaccggtg aacgggctgc
      481 tcggatcgat cgcgatgacg cccacgcgct caccgcgctc gcgcagggcg gcgacgagcg
      541 cgtccgtgag cgtgctcttg ccgacgccag gcgctcccgt aatgccgatg ctacgcgctc
      601 gccccgacag cgggaagagc tcgtgcaaaa gcgcccggta ttcaccgacg cgttcgtcga
      661 cgagacggca ggctcgcgcg agcgccgccg tttcgccccg agcgacgccc gcggcaagct
      721 gcgtcacaga agtcacgata gttccttctg tgacgcttgg gttagttgcg ggcgcgagcg
      781 agggcgtccg cggccatgag gttttcgacc gtgcgggtgg tcatgctgcc gtcacgcacc
      841 tcgggcaaat cgagcaagcg cttttgcagc gggatgttcg tgcggatgcc gctgatgatg
      901 tactcgtcga gcgcgcggcg catgcgccga atcgcctgtt cgcgcgtcgg cgcgtgcacg
      961 atgaccttgg cgagcagcga gtcgtactgc gaaggcaccg tccagccgcc gaacacgccc
     1021 gagtcgacgc gcacacccgc gccgccgggc gggtggtact cgaggatctt tccaggccag
     1081 ggcgcgaaag tgaccggatc ttcggccatg atgcggcact cgatggcgtg cccgcggaac
     1141 ttccacggac gcgtgtcggg cagctcgagg cgctcgcccg cggcggcgcg gatctgctcg
     1201 acgacgagat cgacaccggt cgccatctcg gtgacggggt gctcgacctg cacgcgggtg
     1261 ttcatctcca tgaagtagag cttgccgtgc tcatccatga ggaactcgag cgtgccgagc
     1321 gagttgtaac cggtctcgcg gatcgcgtgg cgaatgcgct cgcccatctc cgcgcgcagc
     1381 gcgtcgttca tcgacgggct cgacgcctct tcgatgagct tctggttctt gcgctgcagc
     1441 gagcattcgc gttcaccgag cgtccacact tggccgtggt tgtcggcaag ggcctggaac
     1501 tcgatatgct tcgggcgctc gacgaagcgc tcgagataga gctccgggtt cttgaacgcc
     1561 gaggcggctt cggacgtgca cgactcgaac gcgcgcctca cctcggagag ttcacgcacg
     1621 atgcgcatgc cgcgaccgcc gccgcctccg cgcgccttca tcatgatcgg gaagccgacg
     1681 cgcttcgcct cgctctccgc gtgctcgacg ttggcgagcg cttcgctgcc gggcaacagc
     1741 ggcagaccga acttttgcgc gatttcgcga gcgcggatct tgtcgcccca gcgcagcatc
     1801 gcttccggcg acggcccgat gaagtgcagc ccgcaacgct gaaccacctg agcaaactcg
     1861 gcgttctccg aaagaaagcc gtagcccggg tgaatcgcat cggcggcggt gatctcggcg
     1921 gcgctgatga tggcggggat gtgcaggtag ctcttcgaag cgagcgcggg gccgatgcac
     1981 accgcctcgt ccgcgaagcg cacgtgcagc gcgccgttgt ccgcctccga gtgtacggcc
     2041 acggtttgaa tgccgagctc gcggcaggcg cgaatgattc gaatcgcgat ttcgccgcgg
     2101 ttggcgatga ggaccttgcg aaacatcggc gctagctctt cttgatcttg aagagcttct
     2161 ggccgaactc cacgctcttc ccgttctcga ccaaaatctc gacgatcgtg ccgccgactt
     2221 cggcctcgat ttcgttcatc agcttcatcg cttcgatgat gcagagcggc tggcccttct
     2281 tcacttgggc gccgacttcg acgaagttct ccgcctcggg ggacggcgct cggtagaacg
     2341 tccccacgaa gggcgaggtg atgaaggcga tgctagcgtc gttcacttcg acggcagcgg
     2401 cggcggcggg agccggcgcg gcggcgggag ccgcgtgcac gaagtgcgct tgagccgctg
     2461 ctgcgggagc gcgaccgaac ccgatcttca gacgaatctt ttcgtcctcg tactcgaact
     2521 caccgacctt cttttcctcg agcgtcttca gcagggcttc gagctgttcc agagcaattt
     2581 gcatgagtct gatttcccgg gatttccaaa cagagcgccg tcgcgcttcg cgtccgagcg
     2641 cagcgaggcc ccggacgtta gcttggcgaa cgcgctctag acaacccgct cgccgttgtc
     2701 gagcctggtt ttgtgggccc tcgcctttcg acgctgtgcg gcattccgag cccagtgcga
     2761 aatgactcgc cgctcgacag aactccgctg cgaaagccga tggacgggtg cgatccgttt
     2821 tacacagcgc cgaggtgctg cacgaccgct cggagcgcga cttcgtagga ggccgctcca
     2881 aagcccgcga tgcgtccgag gcatgccggc gcgacgcacg agtggcgacg aaacgcttcg
     2941 cgggcgtcgg ggttcgagag gtgaagctcg accgtcggca cgccggcgcc cttgatcgcg
     3001 tcgtgtaacg cgtacgaggt gtgcgtgtac gcgccggggt tgatcaagat cgcgccgaag
     3061 ccgtcgtcgg aagcggcacc aatccagtcg atcagcagcc cttcgtgatt cgactggcgg
     3121 cactcgacgc gaacgcccag ctccgcggca accgcttcga ggcgatcgtg aatttgcggc
     3181 agcgtcgtgc ggccgtaaat ttccggctcg cgtcgaccga gccggtcgag gttgggaccg
     3241 ctcagaacga ggacgttcag ggtcgttttc gcggcggcgg tccg
//
LOCUS       DASTKS010000890         5750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1260467, whole genome shotgun sequence.
ACCESSION   DASTKS010000890 DASTKS010000000
VERSION     DASTKS010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260467"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1407
                     /locus_tag="VFQ35_18530"
     CDS             76..1407
                     /locus_tag="VFQ35_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932710.1"
                     /translation="MLRTALTLGFSVFVASSARAATLPDHRPLDVIVISDEVNPNRLT
                     DAELTQPGDISAALNAPDSGLHLVGAGAQEIDSQCADKALERLASDPGPDVVVYFAHR
                     SARSCAGADVQATLTNRIEALLVRGGGFVVFHHGVYQATGKERILELIGAQANSISWS
                     TTEGQRVINVAPQHFVTTNGIAYSEKTTLSAFAGVNAGTYDSFVNVPDERYPQTTLSD
                     VAGATRTVLFASNSGGNRVLSFALSRPTWKGRVVTYQPGEYQPNALDNRRGSNFQILA
                     NAILFSAGRVDEAGNSTDASSSGGAPSAGGQAAGASAGGTATAGASWGGASAGSAQGG
                     TSAGSEPGGRGGDGRGGASTSGAGGTNQASSGAPSTGQSGAEASVGGGGSASASGGRA
                     EVPATEGSSSGCSVLPSRGEGSRVGAIVVASLVLGAFARRRRCHGSSRDRR"
     gene            complement(1342..2121)
                     /locus_tag="VFQ35_18535"
     CDS             complement(1342..2121)
                     /locus_tag="VFQ35_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932711.1"
                     /translation="MPMKAAVGSSLVQLGIPTIALILAALFVWGVAHTSKQAELAPGA
                     WRRHALYALLGVTAFMGGIALLALSGALARFDLKPPPMILWMTATLGSALGVGLSKAG
                     RRLAEGLPFAALVGFQAFRLPLELVMHRAASEGVMPTVMSYSGYNFDIVSGSLALALG
                     IALARGNVPRGFLVVFNAVGLLLLTVIVVIAFLATPLVRAFGDDQLNVWITRFPYAWM
                     SVMVASALLGHVLLLRKLGLSPAVARTPVATPPSGESAEYQ"
     gene            2310..3002
                     /locus_tag="VFQ35_18540"
     CDS             2310..3002
                     /locus_tag="VFQ35_18540"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9932712.1"
                     /translation="MQKRRKYKQFCGLARALDRIGERWTLLIVRNLLLGPKRYSDLAE
                     ELPGITTNLLALRLREMERQELIVKRKAPSPVRATVYELAPLGYALEPALMELARWGG
                     RFLDQPKKEDTLNVGWGLLALKRRYRGGLTQSVELTVGDRCFELTMTPAYLDVKERSA
                     TRPDVKVRGDLGAVRGFLFQGADAELMRKRGELVVDGSEAAWQAFKQAFVPREPSSEL
                     IAARLASRGVLE"
     gene            3300..4157
                     /locus_tag="VFQ35_18545"
     CDS             3300..4157
                     /locus_tag="VFQ35_18545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9932713.1"
                     /translation="MIERQDFGRTGHASSRVIFGAAALWQVTQSEADRTLEVLLRHGV
                     NHIDTAASYGDSELRIGPWMREHRRSFFLATKTGKRTYTEAKEEFQRSLERLRVDQVD
                     LLQLHCLVDPGEWDTAFGAEGALEALLEARENGLTRFIGVTGHGVTVAAMHRRSLARF
                     EFDSVLLPYNPVMIENAAYAADFEALLLECTRRRIAVQTIKSIARGAWPSEQHSRATW
                     YEPLEAEADIELAVRWVLARREVFLNSVGDIHLLPKVLDAAARFSAAPSPADVQALVQ
                     RANIQPLFT"
     gene            complement(4245..5057)
                     /locus_tag="VFQ35_18550"
     CDS             complement(4245..5057)
                     /locus_tag="VFQ35_18550"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9932714.1"
                     /translation="MTSLDSRFVGSIPEIYDGILVPMIFEVYAKDLAEQVANSGALAV
                     LETAAGTGALTRALAARLSTAARLLATDLNQPMLDYGAARAPADARVSRRQADALALP
                     YEGETFDAVACQFGVMFFPDKVQGYCEARRVLRPGGRFVFNVWDALSENTFPSAVSEA
                     LADYFPHDPPRFMQRTPHGYYDLDQIRRQLELAGFTLVTAEAADHVSRARSARDIAVA
                     FCQGTPLRNEIEARNPGGLEAATDHVAEAVARRFGEGACEGKLRAILVTAQA"
     gene            complement(5067..5618)
                     /locus_tag="VFQ35_18555"
     CDS             complement(5067..5618)
                     /locus_tag="VFQ35_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET9932715.1"
                     /translation="MSTNIERIAVKTIEGAESNLGAYAGKVRLVVNVASKCGLTPQYT
                     ALEGLYRKYHDRGLEVLAFPANEFGGQEPGTDEEIRTFCDTRYDVTFPLFSKIVVKGP
                     GQHPLYAELTQAQPVATEKPGSDFKAKLRQHGVDLGAPQDITWNFEKFLVDRQGNVVT
                     RFAPDVPPDDALVTNAIERALES"
BASE COUNT         1033 a         1786 c         1910 g         1021 t
ORIGIN      
        1 gacctcgcat gccgacggga gctttcaccg cacaattgct caaagcgttg gcggttgggt
       61 gtaaggtctg agggcatgct tcgcaccgcg ctcacgttgg gcttctcggt attcgtcgcg
      121 agctcggcgc gtgcggcaac gctacccgac catcgcccgc tcgatgtcat cgtcataagc
      181 gacgaggtca atccgaatcg gctcaccgac gccgagctca ctcaaccggg agacatttca
      241 gcagccctca acgcccccga cagcgggctg catctcgttg gagcgggggc tcaggagatc
      301 gatagccagt gcgctgacaa ggccctcgag cggcttgcgt ccgacccagg gcccgacgtc
      361 gtggtgtact tcgctcaccg ctccgcccga tcgtgcgcgg gcgcggatgt ccaagcaacc
      421 ttgacgaacc gcatcgaggc gctgctcgtt cgcggcggtg gcttcgtcgt ttttcatcac
      481 ggcgtctatc aagcgaccgg caaggaacgc atcttggagc tcatcggagc ccaggcgaat
      541 tctatcagct ggagcacgac cgaaggtcaa cgcgtgatca acgtcgcccc gcagcacttc
      601 gtgacgacga acggtatcgc ctattcggaa aagacgactc tgtccgcgtt cgccggtgta
      661 aacgcgggta cctacgactc gttcgtgaac gtccccgacg agcgctatcc gcaaaccaca
      721 ctgagcgacg tagccggtgc gactcggacc gtgcttttcg catcgaattc cggtggtaac
      781 cgggttttga gcttcgctct ctctcgaccc acgtggaagg ggagggtcgt cacgtaccaa
      841 ccgggcgagt atcagcccaa tgcgctcgac aaccgccgcg gtagcaactt tcagattctg
      901 gcgaatgcga ttttgttctc cgctggccgc gtcgacgagg cggggaactc gaccgacgcc
      961 tcgtcgagtg gaggagcgcc cagtgctggt ggccaggcag caggggcgtc ggcgggcggt
     1021 accgcgactg cgggggcatc ctggggtggt gcgagcgccg gtagcgcgca agggggtaca
     1081 tcggctggga gcgaacccgg cgggcgaggg ggagacgggc gaggcggtgc gtcgacgagc
     1141 ggcgcaggcg ggacgaacca ggcgagtagt ggagcgccga gcactgggca aagcggcgcc
     1201 gaagcgagtg tcggaggggg aggctcggcg agcgcttcgg gaggccgcgc cgaagtgccc
     1261 gctaccgaag gctcgagctc cgggtgcagc gtgctgccgt cgcgaggcga aggctcccga
     1321 gtcggcgcga tagtcgtggc ttcattggta ctcggcgctt tcgcccgaag gcggcgttgc
     1381 cacgggagtt cgcgcgaccg ccggtgaaag accgagtttt ctgagcagca gcacgtgccc
     1441 gagtagcgcc gacgcgacca tcaccgacat ccaagcgtag gggaaccgag tgatccagac
     1501 gttcagctgg tcgtcgccga acgcgcgcac gagcggggtt gccaggaacg cgatcaccac
     1561 gatgacggtg agcaagagaa gaccgacggc gttgaacaca accaagaacc cacggggcac
     1621 gtttcctcgc gccagcgcaa ttccgagcgc gagcgccaac gagccgctca cgatgtcgaa
     1681 gttgtagccg ctataagaca tcaccgtcgg catcaccccc tccgacgccg ctcgatgcat
     1741 cacgagttcg agaggcaacc ggaacgcttg gaagccgacg agcgcagcga acggcaaacc
     1801 ctccgctagc cgccgaccgg ctttcgaaag cccgacgccg agcgcgctgc cgagcgttgc
     1861 cgtcatccaa agaatcatcg gtgggggctt caggtcgaag cgagcgagcg cgcccgagag
     1921 cgcgaggagc gcgattccgc ccatgaaagc ggtgacgccg agcagcgcgt aaagagcgtg
     1981 tcttcgccag gcacccgggg cgagctccgc ttgcttactg gtgtgggcta ctccccacac
     2041 gaacagcgca gccaaaatca aagcaatcgt tggaatcccg agctgcacca ggctggagcc
     2101 aacggctgct ttcataggca tgtgctcacc gccggcgtcg taccgaaaga cctggaagca
     2161 cagcggcaaa accccctttc acgagctcgc ccccaaacga aatgtcgtgc gcgttcgaac
     2221 tcgcccgatg gaccgaagga atgaggcatg cgggcattgt actttagttt ttattagcga
     2281 tgaatgtaat ttgtgatagt catgtcaatg tgcaaaagcg gaggaaatac aagcagtttt
     2341 gtggcctggc gcgagcgctg gaccgaatcg gcgagcgctg gacgctgctc atcgttcgta
     2401 acttgctcct tggccccaag cgttactcgg acctcgcgga agagttgccg gggatcacga
     2461 cgaacctgct cgcgctccgg ctgcgtgaaa tggagcgcca agaactcatc gtcaaacgca
     2521 aagccccgtc tcccgtccgt gcaacggtgt acgagctcgc accgctcgga tacgcgctgg
     2581 aacccgcact gatggagctt gcgcgttggg ggggacgctt tctcgaccag cccaaaaagg
     2641 aggacacgtt gaacgttggt tgggggttac tcgcgctcaa gcgccgatat cgaggtggtc
     2701 tcacgcagtc agtcgagctc accgtcggtg accgttgctt cgagctcacc atgacaccgg
     2761 cgtacctcga cgtgaaagag cgctcggcga cccgtcccga cgtgaaggtg cggggagacc
     2821 tcggcgcggt acggggcttc ctatttcaag gcgccgacgc ggagctcatg cgcaagcgcg
     2881 gggagctcgt cgtcgacggc tcagaagccg cgtggcaggc gttcaagcag gcgttcgtgc
     2941 cgcgagaacc gagttccgag cttatcgcgg cgcggctcgc ttcgcgcggc gtgttggagt
     3001 gaccacgagc tttatcgcgg cgcggctcgc ttcgggcggc gcgttggagt ggccgcgcgg
     3061 tgggagcccg ggtttgcgcg tcactcgggt acgccgcgga gcaggttctg tctcgcgctc
     3121 cagtagtctc gccacgggtc aacgtgtgac gcggtccgcg tcagcaggcg agcgagtcct
     3181 cggtcggtcg acgcgtaatg tgagcgtaat agaccgaaat cagagccggt cgagctgagt
     3241 gctccgcgga caggaacgcc acgctggcga gtcggcgcac aaagaggcac gctggcggca
     3301 tgatcgagcg gcaagacttc ggtcgtaccg ggcacgcgag ttcgcgagtg atcttcggcg
     3361 cggcagcgct ctggcaggtg acgcaatccg aggcggatcg caccctcgaa gtgttgcttc
     3421 gccacggtgt gaaccacatc gacaccgcag cgagctacgg tgactcggag cttcgaatcg
     3481 ggccgtggat gcgtgagcat cgccgctcgt tcttcttggc aaccaagacc ggcaagcgga
     3541 cttacaccga ggccaaggag gagtttcaac ggtcgctcga gcggttgcgc gtcgaccagg
     3601 tggacttgct tcagcttcat tgtctcgtgg atcccggcga atgggacacc gctttcggcg
     3661 ccgagggtgc gctcgaagcc ttgctcgaag cccgcgaaaa cgggctgacg cgcttcattg
     3721 gggtgaccgg gcacggcgtc acggtcgcag ccatgcaccg tcgctcgctc gcgcgcttcg
     3781 agttcgactc cgtgctcttg ccctacaacc ccgtgatgat tgaaaacgcc gcctacgcgg
     3841 cggacttcga ggcgctgctg ctcgaatgca cgcgccggcg tatcgcggtt caaaccatca
     3901 aatcgatcgc gcgcggcgcg tggccgagcg agcagcactc tcgcgcgact tggtacgagc
     3961 cgctcgaggc cgaagccgac atcgagctcg ccgtccgctg ggttctggcg cgccgggaag
     4021 tcttcttgaa ttcagtcgga gacattcacc tgctgccgaa ggttctcgac gcggctgcgc
     4081 ggttcagcgc ggcgccttct ccggccgacg tgcaggcgct cgtccagcgc gcgaacatcc
     4141 agccgctgtt cacgtaacgg cgtgctgctc gatgagcacg cccgtcccgc tgcgcgtcgg
     4201 cgtgctcgcg agcgacccgt tcctgcgcaa gcgctggaat ggttctaagc ttgcgccgtc
     4261 actaggatgg cgcggagttt cccttcgcac gccccttcgc caaagcgtcg cgcaaccgct
     4321 tcggcgacgt ggtcggtagc cgcctctaat ccgccagggt tacgcgcttc gatttcgtta
     4381 cgcaacgggg tgccctggca aaacgcgacc gcgatatcgc gcgcggaccg agcgcgactg
     4441 acgtggtccg ccgcttctgc ggtcacgagc gtgaaacccg cgagctcgag ctgccgtcga
     4501 atctggtcga ggtcgtaata tccgtgcggc gttctttgca tgaaccgcgg cgggtcgtga
     4561 gggaagtagt cggccaacgc ttcgctgacc gcgctcggga aagtgttctc ggagagcgcg
     4621 tcccacacat tgaaaacgaa gcgccctcct gggcgcagaa ctcggcgcgc ctcgcaataa
     4681 ccttggacct tgtctgggaa gaacatcaca ccgaactgac aggcgaccgc gtcgaaggtc
     4741 tcgccctcgt acggtagtgc caaggcgtcc gcctgccggc gcgacacccg tgcgtccgct
     4801 ggggcccgcg ccgcgccata gtcgagcatc ggctgattca agtcggttgc caggagccga
     4861 gcggcggtcg acaggcgtgc agcgagcgcg cgggtcagcg ctccggtgcc agctgcggtc
     4921 tcgagaacgg caagcgcccc cgagttcgcg acctgctctg cgaggtcctt cgcgtagacc
     4981 tcgaagatca tcggcaccag gatgccgtcg taaatttctg ggatcgagcc gacgaagcgc
     5041 gaatcgagcg atgtcatggg cgcctctcag gactccaggg ctcgttcgat cgcattcgtt
     5101 acgagcgcgt cgtccggggg tacgtcgggg gcgaaacggg tgacgacgtt gccttgccta
     5161 tcgacgagga acttctcgaa gttccaggtg atgtcttgcg gcgcgcccaa gtcgactccg
     5221 tgctgccgta gcttcgcttt gaagtcgctg cccggctttt cggtagcgac aggctgtgct
     5281 tgggtgagtt cggcgtaaag cgggtgttgg ccggggccct tcacgacgat cttggagaaa
     5341 agggggaagg tgacgtcgta ccgcgtgtcg caaaacgtgc ggatttcttc gtcggttcct
     5401 ggctcttgcc cgccgaactc gttcgccggg aacgcgagca cttcgaggcc gcgatcgtgg
     5461 tacttgcggt aaaggccttc gagggccgtg tattgcggag tgagcccgca cttggaggca
     5521 acgttgacca cgagcctcac cttgccggcg taggccccca agttcgactc agcgccttcg
     5581 atcgtcttga ccgcgattcg ttcgatgttc gtgctcatga gccctctgct tcgagggcac
     5641 cttagcaccg cgcgacgcgc ggggcggttc gcgcttcgtg gggctatttg gcagtaacgc
     5701 ggaccgaaac gcggcggttt ttgctcttac acgcctcggt atcgttcgct
//
LOCUS       DASTKS010000891         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1260625, whole genome shotgun sequence.
ACCESSION   DASTKS010000891 DASTKS010000000
VERSION     DASTKS010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260625"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VFQ35_18560"
     CDS             <1..1572
                     /locus_tag="VFQ35_18560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HET9932716.1"
                     /translation="PLRIAPGTRISVYRVDDVIGEGGMGVVYRAYDEALDRVVALKCL
                     HTNLAGDADIRRRFEREARVLRPYSHPGVVSVYDFVEHDYLLAMVMELVEGPSLVQHL
                     EKWRGHMPLDEVRHLFCGVLDAVAAGHKSGVIHRDLKPENILVMNTGDELRPKIVDFG
                     IARILEGTTYTMTGAFLGTCCYMSPEQVQRPHAADPRSDIYSLGVTLYQLCTGRLPFE
                     GNHFAVMMAHVNEKPRRPSELRADIPEALEKLILDALAKDPSARPQTCRAFRERLEEA
                     LPERTPSSAPSTRPLPPLVKGADGRDMVLIPAGGFRMGRSKRNVHLDAFYIDRTPVTN
                     REFLTFLEVTGYRPTDPSAGRFVPHVLNGKLSKREEDHPVVNVSWFDARAFAAWAGKR
                     LPSEAEWEKAARGVDGRKYPWGRGEPNKYRANYGNKAGGTARVGSFPEGASPYGVLDL
                     AGNVWEWCDDYDDPSFYEDGPPNNPRNQNASERVVMRGGSYLFGVQALRTYARTSFEP
                     HYRFAGGGFRCARSA"
     gene            1917..>3196
                     /locus_tag="VFQ35_18565"
     CDS             1917..>3196
                     /locus_tag="VFQ35_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9932717.1"
                     /translation="MADLDAVRVAARQVRAHFQPIVDLLGGTVLGYEVLSRAPRFPSI
                     ADAFVLARRHGLGWDFEAACRQAAIAQILELNGRARGLRFFLNVGPEVLSDPRFVSVF
                     TGPALREAGLEPSRVVLELTEQQAVTDRSRFTELIQHYTNQGFEIAIDDFGVGHSSLV
                     TLVSSTPHFLKLDLELVRGIHTDAYRQRLVRSLVGFAANVDAKLVAEGVESWEELEVL
                     VRLGLRYAQGFLFAKPAEQLQSLDPEQRTSVRKLMRQENAWHSELGECISQLVTGAPT
                     VTMGGVSCGDVARSYQRDAALDEIVVLDDCDRPVGLLTRQHFFEKVQTPDGGRLDSLF
                     VHEVAKSHFLMVEASTPVTSLSRLAMERARADLYDPVVVVGAEGKYLGTVTIRNLLVR
                     SAELETQSAEGANPITGLAGHRAVLCWLTSVLSRP"
BASE COUNT          534 a         1033 c         1034 g          595 t
ORIGIN      
        1 ccgctgcgca tcgcgcccgg aactcgcatc tccgtctacc gcgtcgacga cgtcatcggc
       61 gaaggtggca tgggcgtcgt gtaccgggcg tacgacgagg cgctcgaccg agtcgtcgcg
      121 ttgaagtgcc tgcacacgaa cctcgctggc gacgcggaca ttcggcgccg gttcgagcga
      181 gaagcgcgcg tgctgcgccc gtattcgcac cccggcgtcg tcagcgtcta cgacttcgtc
      241 gagcacgact acctgctcgc gatggtcatg gagctcgtcg aagggccgag cctcgtgcag
      301 cacctcgaaa agtggcgcgg ccacatgccg ctcgacgagg tgaggcactt gttctgcggc
      361 gtgctcgacg ccgtcgcggc cgggcacaag tctggagtca ttcaccgcga cttgaagccc
      421 gagaacatcc tcgtcatgaa caccggcgat gagctcaggc ccaagatcgt cgactttgga
      481 atcgctcgca tcctcgaggg tacgacgtac accatgaccg gcgccttcct tgggacgtgt
      541 tgctacatgt cgccggagca ggtgcaacgg ccgcacgctg ccgatccgcg ctccgacatt
      601 tattcgctcg gcgtcacgct gtaccaacta tgcacgggcc gattgccttt cgaaggcaac
      661 cattttgccg tgatgatggc ccacgtgaac gagaagccgc gccgcccgag cgagctccgc
      721 gccgacatcc cggaagcgct cgaaaagctg atcttggacg cgctcgcgaa agacccgagc
      781 gcgcgcccgc aaacttgccg agcttttcgc gaacgcctgg aggaagcgct gcccgagcgc
      841 acgccgagct ccgcgccttc gacgcgaccg ctaccgcctc tcgtcaaagg tgcagacggg
      901 cgcgacatgg tcctgatccc ggctggcggc tttcgcatgg ggcgcagcaa gcgcaacgtg
      961 cacctcgacg ccttctacat cgatcgcacc cccgtcacga accgcgagtt tctgaccttc
     1021 ctcgaggtca ctgggtaccg tcctaccgat ccgagcgcgg gccgcttcgt gccgcacgtg
     1081 ctgaacggca agctgagcaa gcgggaagaa gaccacccgg tcgtgaacgt atcctggttc
     1141 gacgcgcgcg cattcgcggc gtgggccggc aaacgcttgc catccgaagc ggagtgggaa
     1201 aaggcagctc gcggcgtcga cggtcgcaag tacccgtggg gacgcggtga gccgaacaag
     1261 taccgcgcga actacggcaa caaggcgggc ggtacggcgc gagtcggctc gttccccgaa
     1321 ggggcgtcgc cgtacggagt cttggatctg gctggcaacg tgtgggagtg gtgcgacgac
     1381 tatgacgacc cctcgtttta cgaggacggt ccgcccaaca acccgcgcaa ccaaaacgcg
     1441 agcgagcgcg tcgtgatgcg cggcggctcg tacctcttcg gagtgcaggc cttgcgcact
     1501 tatgctcgga cgagcttcga gccgcattac cgctttgcgg gcggtgggtt ccgctgcgcg
     1561 cggtccgcgt gactcgcgtc gcaccgcgac cgatgcgcgc acattcgggg tagaggtcgc
     1621 aagttctccc actcgcaggt tgtatcgaaa tgcgacgagt ctcgtcacgc gcgtcccgat
     1681 tgcggtcgtc agtgacccaa cgatcgcgaa gttgaaacgt tcgcgtcgaa taactctttg
     1741 gaattaggaa acgcgaagtc ggtcgcggcg atgcgcgacc attgctcgcg gattagttga
     1801 gtaagcgctg gcgcacgtgt taagttgtgc gtcggtcgtt cgggatcatg tcgagtcccc
     1861 gcttcgaacc tcctgttgcg gccttagctg cgaacgcttc ggcgctgcca tccgcagtgg
     1921 ccgacttgga cgccgtgcgg gtagcggcgc gtcaggtgcg agctcacttt cagcccatcg
     1981 tcgatttgtt gggcggaacc gtgctcggct acgaggtgct gtcgcgcgcg ccgcgcttcc
     2041 ccagcatcgc ggatgccttc gtcctggcgc gtcggcatgg cttggggtgg gatttcgagg
     2101 cggcttgtcg gcaggccgcc atcgcgcaga tcctcgagtt gaacggccgc gcgcgcggct
     2161 tgcgcttttt cctcaacgtc ggcccggagg tgctgagcga cccgcgtttc gtttcggtat
     2221 tcaccggccc cgcattgcgc gaggcggggc tcgagccgtc gcgagtggtg ctggaactca
     2281 ccgagcagca agcggtgact gatcgctcgc gcttcaccga gctcatccaa cactacacga
     2341 accaaggctt cgagatcgcg atcgacgact tcggcgtggg tcactcgagc ctcgtcacgt
     2401 tggtgagctc gacgccgcat ttcttgaagc tcgatttgga gctcgtccga ggaatacata
     2461 cggacgctta tcgtcagcgt ctcgtacgtt ctttggtcgg cttcgctgcg aacgtcgacg
     2521 ccaagctcgt ggctgagggc gtcgagtctt gggaggagct cgaggtcttg gttcgcctcg
     2581 gtctgcgcta cgcccagggc tttttgttcg ccaagcccgc cgagcagctc caaagtctcg
     2641 accccgagca gcggacgtcc gtacgcaagc tcatgcgcca ggaaaacgct tggcacagcg
     2701 agctcgggga gtgcatcagc cagctcgtca ccggcgcgcc gactgtcacc atgggtggcg
     2761 tcagttgtgg cgacgtcgcg cgcagttacc agcgcgacgc agccctcgac gaaatcgtcg
     2821 tgctcgatga ctgcgatcgg cccgtcgggt tactcacccg ccaacacttc ttcgagaagg
     2881 ttcagacacc cgacggcggt cggctcgact cgttgttcgt ccacgaggtg gcgaagtcgc
     2941 attttctcat ggtcgaggca tcgacgccgg ttaccagctt gtcgcgcctc gccatggagc
     3001 gcgcgcgcgc cgacctgtac gaccccgtcg tcgtcgtggg tgcggaaggg aagtacctcg
     3061 gaacggtcac gattcgcaat ctgttggttc gctcggccga gctcgagacg caatccgccg
     3121 agggagcgaa tccgatcacc gggctcgccg gccaccgcgc ggtgctgtgt tggctcacga
     3181 gcgtgctttc ccgccc
//
LOCUS       DASTKS010000892         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_144295, whole genome shotgun sequence.
ACCESSION   DASTKS010000892 DASTKS010000000
VERSION     DASTKS010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144295"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFQ35_18570"
     CDS             complement(<1..795)
                     /locus_tag="VFQ35_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LDL receptor domain-containing protein"
                     /protein_id="HET9932718.1"
                     /translation="MLIRGSKGTLTLLALLASPTFVAASCDDNLTIGTDGHAAGAGGG
                     MVIDGKGGSSAAGKPGIGYGGKNGAGGSPNQMSFVCNDGKRIPASSVCDTKKDCGDGS
                     DEVKCPMTGPGPFQCADGSKIYIAPELICDGKADCPDKSDEMGCTPNAGLFYCKDMSS
                     IPSALACNGKPDCKDGSDEVNCAPAVFYCKDMSKILATSVCDGKADCKDGTDELNCAP
                     EVFYCKDMSKIPATSVCDGKADCKDGTDELNCAPEVFYCKDMSKIPA"
     gene            complement(880..1788)
                     /locus_tag="VFQ35_18575"
     CDS             complement(880..1788)
                     /locus_tag="VFQ35_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932719.1"
                     /translation="MTGLSREARALLERARAQHEPTPRDRESVLRGLGPKLPAAAALA
                     SATAHTLSAAASTAPVASATAALVSKWLVGGLLLGALGAGSGTLIERATGDSKPSASP
                     VASARRATKLASASPLSSAAPTTLGVPMPIDTSEQSSSEASKPVSTGAGPFVPGASGG
                     GNRLSKQQASPPAVVNPAAARTAVAAFPEPPSQNPEGASAMARSRSAPQPTRSADLLV
                     EARALRDAQTALDEGRAAEALALVDAQSKQFANGELQEERRAARLLALCSLGKSRLVE
                     REIAAFLESSSASPLAPRVRKACRSR"
     gene            complement(1785..2348)
                     /locus_tag="VFQ35_18580"
     CDS             complement(1785..2348)
                     /locus_tag="VFQ35_18580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9932720.1"
                     /translation="MSLVRPLADLTPIGGCEASFQDLYEAHFSFVWRTLRRLGVQPDN
                     LSDATQDVFFVVGRRLGDLHSPELGRSWIYGIVVRVAHEYRRSQRRSLHEEAEFEDML
                     DPKSRSAHEHAEHAEEVELLDRLLSGLDHDKREVLVLVELEQLSVPEVAQLTGLNLNT
                     VYTRLRAARIAFNEAVVRHRARTRSRP"
     gene            complement(2391..3377)
                     /locus_tag="VFQ35_18585"
     CDS             complement(2391..3377)
                     /locus_tag="VFQ35_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932721.1"
                     /translation="MRARIEPPSCYKLPPKDGRLDQRQAWVIWGSAWIVLLMAGSARA
                     LSRTVALTSNAPSECESAEHIEVSVDELVRRAQGEPLRASLSIVRTAAGYSATLQIHG
                     TEPRSLSAPSCEAVVEAVTVILALAIDPQANTHTRRAPKSSPEEPKPAVERRPSVFRF
                     DAGAASTFDTSTLPHVALGASAKAGLWAPRWSIDAVATYWLPASRSLPNDPTVGGRFT
                     WWTVGISGCVAFAERPYLAACVEPELGRLEGSGSGTLDDRSVGATWLAVSAAPAVSLD
                     LTRHFKFRAALGPALTLLGRHAFVLERSGENLEVHRPGRLSLRGRFGLDVLF"
     gene            complement(3427..4233)
                     /locus_tag="VFQ35_18590"
     CDS             complement(3427..4233)
                     /locus_tag="VFQ35_18590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9932722.1"
                     /translation="MKAANFGALMPHLVHAGTRIHYSVMGEGPPLLLHHGSGSNGAVW
                     VQHGYVRALRSRYRLILLDARGHGQSDKPHERTSHTLELRVGDVAAVLDALDVERAHF
                     FGYSMGGWIGFGMAKHAPERLRSLVIGGAHPFADPFVSGVELEDPNDSGAFLRAMALV
                     LGEPISAENERFLLRNDALAVVASLQERVDLGEVLPRIQVPCLLFAGSKDRRYQLIEQ
                     TAHGVPNARFVSLPDLGHVAALAASDLVLPHVTSFLSAADSPGPLPFAGR"
     gene            complement(4300..4818)
                     /locus_tag="VFQ35_18595"
     CDS             complement(4300..4818)
                     /locus_tag="VFQ35_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9932723.1"
                     /translation="MANVWERTTEKALELVKASEAAFQGLTGVFKHLMQEHGELAALV
                     KRARRCSDADERARLYAELRRRLVAHERAESAEVYRALAEHESTIRLAMAHGDQVREL
                     ESVLERLDRLKPADPEWERAMELLAQTVEQHVFDEEGDYFPRGQAALGDESAQALTER
                     YEAAKQRELGAT"
     gene            5300..6163
                     /locus_tag="VFQ35_18600"
     CDS             5300..6163
                     /locus_tag="VFQ35_18600"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9932724.1"
                     /translation="MANETESSAGPGNHILGALPQAEIDAIAPHLEPMLLDQRHVAYD
                     VNTPIEYVYFPEDCVISLVSVMRDGQVVEVATVGNEGMIGLPLFLRARRTLGQAFCQV
                     PGSAFRMKADAFVEFAAKGKLNDLMHRYTQALFAQVSQSAACNRLHLAEERFSRWILM
                     THDRVRGNDFALTQDFLSQMLGVRRATVSEVAAQAQRDGLIRYHRGHMTIVNRKGLEE
                     RACECYSIIQAEFEQLIGDSSKNSLSRARVSLPSFSRAGKATAGEAIPHIDRTEAGSP
                     APTPADNNAST"
     gene            6237..6983
                     /locus_tag="VFQ35_18605"
     CDS             6237..6983
                     /locus_tag="VFQ35_18605"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9932725.1"
                     /translation="MTNKLGWIGLAALGLCGACASHDRAPAASAANTSAANVDAATTN
                     PNTGPNFAPARADGTASATATPGRDTPPGTNPAPGTGDTIVDRDRSMTGSARNARSTT
                     SATSSSTDTSASSDTNSGYAGSSDPAQRADTNNGPSATSKNTDTSAPDNTRVNKRDRN
                     DAAPLPTDQGNNEADLKTTQAIRQALMKDKNLSFGAKNVKIITQNGRVTLRGPVSTEA
                     ERSAIEAAARKVAGANQVDNQLELKNDQRK"
BASE COUNT         1306 a         2350 c         2273 g         1174 t
ORIGIN      
        1 cgcggggatc ttcgacatgt ctttgcagta gaaaacctcc ggcgcacagt tgagctcgtc
       61 ggtcccgtcc ttgcagtccg ccttcccgtc gcataccgac gtcgcgggga tcttcgacat
      121 gtctttgcag tagaaaacct ccggcgcgca gttgagctcg tcggtcccgt ccttgcagtc
      181 cgccttcccg tcgcacaccg acgtcgcaag gatcttcgac atgtctttgc agtagaacac
      241 tgcgggcgca cagttcacct cgtcgctgcc gtctttgcag tctggcttgc cgttgcacgc
      301 gagcgccgag ggaatcgaag acatgtcttt gcagtaaaag agtcccgcat tcggggtgca
      361 gcccatctcg tcgctcttgt ccggacagtc tgccttgccg tcgcaaatga gctcgggcgc
      421 gatgtaaatc ttgctaccgt cagcgcactg gaagggcccc ggtcccgtca tggggcattt
      481 gacttcgtcg ctgccgtcgc cgcagtcttt cttcgtatcg cagaccgagg acgcggggat
      541 gcgcttgccg tcgttgcaga cgaacgacat ctgattgggg ctgccgccag ctccgttctt
      601 gccaccgtat ccgattcccg gtttaccagc cgcgctgcta ccacccttcc catcgatcac
      661 catgccgccg cccgctccag ccgcgtgccc gtcggtgcca atggtcaagt tgtcatcgca
      721 actggccgcg acgaacgtcg gcgaagccag gagagccagc agagtcaggg taccctttga
      781 accgcgtatg agcatcggtt tttcctcgtc gaattttcgg ggtggcgatc cgttcccttg
      841 gctatgtgag gtcgcagggg tttccgtttc gaattctttt caccggcttc tgcacgcttt
      901 gcgaacgcgg ggcgcgaggg gagacgcgct cgacgattcc aagaacgcgg cgatctcacg
      961 ctcgacgagc ctcgactttc cgagcgagca gagcgcaagc agccgggccg cacgtcgctc
     1021 ctcttggagc tcgccgtttg cgaattgctt cgattgcgcg tcgacgagcg ccagagcttc
     1081 ggccgcccgc ccttcgtcga gagccgtttg cgcatctcgc agcgcgcgag cttcgaccaa
     1141 aaggtctgcc gagcgagtcg gctgcggagc gctccgcgag cgagccatcg ccgaagcgcc
     1201 ttcggggttt tgcgacggcg gctccgggaa ggctgcgacc gctgtcctcg ccgcagctgg
     1261 gttcacgacg gctggcggag aggcctgttg tttgctgaga cgattgcccc cacccgacgc
     1321 gccgggtacg aagggcccgg ctccagtcga cacgggtttg gaggcttcac tcgaagattg
     1381 ctcggacgtg tcgatgggca tcggcacgcc gagggtggtc ggtgcagcgg acgaaagcgg
     1441 cgaggcagaa gccagcttcg tcgcgcgacg ggccgaagca acgggcgaag cgctgggctt
     1501 cgagtcgccg gtggcgcgtt cgatgagcgt cccggaaccg gcgccgagcg cgcccagcag
     1561 aagcccaccg accagccatt tgctcaccaa ggcggcggtc gcgctcgcga cgggcgcggt
     1621 gctcgcagca gcggagagcg tgtgcgcggt ggcgctcgcg agcgctgcgg cggcaggaag
     1681 cttaggcccc aaaccgcgca gcaccgactc tcgatcgcgc ggggtgggct cgtgctgagc
     1741 acgagctcgc tcgagcagcg cgcgcgcctc gcgactcaaa ccggtcatgg tcgactcctc
     1801 gtgcgggcgc ggtgacgcac gaccgcctcg ttgaaggcga tacgggcggc gcggagccgc
     1861 gtgtagacgg tattcaggtt cagcccagtc agctgggcga cctctggcac gctgagctgc
     1921 tcgagctcga ctagcaccaa gacctcgcgc ttgtcgtgat cgaggccgct gagcaagcgg
     1981 tccaaaagct caacttcttc ggcgtgctcg gcgtgctcgt gggcgctgcg cgacttcggg
     2041 tcgagcatgt cttcgaactc cgcctcttcg tgcaacgagc gccgttggga gcgccggtat
     2101 tcgtgagcga cacgaacgac gatgccgtaa atccaggaac gtccgagttc cggcgaatga
     2161 aggtcgccga gcctccttcc cacgacgaag aagacatctt gggtggcatc gctcaaattg
     2221 tctggctgaa ctcccagtcg gcgcagggtg cgccaaacga acgaaaagtg cgcttcgtac
     2281 aggtcctgaa acgaggcttc gcacccgccg atgggcgtga ggtcggcaag cggtcggacc
     2341 aagctcatcg aggctgcaaa ggggctatgc gcgcgcaccg gcgcttccgt ttagaaaagc
     2401 acgtcgagcc cgaaccgccc gcgcaacgaa agccgcccgg ggcggtgcac ctctaggttc
     2461 tcaccggaac gttccagcac gaacgcatgg cgacccaaaa gcgtgagcgc gggaccgaga
     2521 gccgctcgga atttgaagtg ccgagtcagg tccagcgaga cggcgggtgc agcggacacg
     2581 gcgagccacg tcgcgccaac gcttcgatca tcgagcgtac cggaccccga gccttccaag
     2641 cgcccgagct cgggttcgac gcacgccgcc agatagggcc tctccgcgaa cgcaacacaa
     2701 cccgaaatgc cgaccgtcca ccaggtgaag cggccgccga cagttgggtc gttaggcagc
     2761 gaacgactcg ccggaagcca atacgttgcg accgcgtcga tggaccagcg aggcgcccaa
     2821 agcccagctt ttgcggaggc gccgagcgcg acgtgcggca acgtcgaggt gtcgaacgtc
     2881 gaagcagcgc cggcgtcgaa gcgaaagacg ctcggacgtc gctcgaccgc cggtttcggc
     2941 tcctccggcg aggacttcgg cgcgcgacgg gtgtgcgtat tcgcctgcgg gtcgattgcg
     3001 agcgccagga tgaccgttac cgcctcgacc accgcttcgc agctcggcgc cgacaaactg
     3061 cgcggctcgg tgccatgaat ttgcagggtc gcggagtagc ccgcggccgt tcggacgatg
     3121 ctcaagctcg ctcgcagcgg ctcgccctgc gctcggcgca cgagctcgtc gacgctcacc
     3181 tcgatgtgct ccgcgctctc gcactcgctc ggcgcgttcg acgtgagggc taccgttcga
     3241 gagagagccc gcgccgaacc ggccatgagg agcacgatcc acgccgaccc ccagatgacc
     3301 catgcctgcc tttggtccaa gcgcccgtcc ttcggcggca acttgtagca cgacggtggc
     3361 tcgattcgcg cgcgcacgat ccggccgagg caaaccgacc cgatgatcgg agcgagccgc
     3421 gaacggttaa cgacccgcaa atggcagagg gccaggagag tcggcggcgc tcaagaacga
     3481 agtaacgtgc ggaaggacga ggtctgaagc cgcgagcgcc gccacgtgtc ccaggtcggg
     3541 gagagacacg aaccgagcgt tggggacgcc gtgagcggtt tgctcgatca gctgataacg
     3601 gcgatcttta ctgccggcga agagcaagca gggcacctgg attcggggaa gaacctcgcc
     3661 gagatcgacg cgctcctgga gagaggcgac gaccgcgagc gcatcgttgc gcagcaaaaa
     3721 tcgttcgttt tcggcggaga tgggctcacc cagcacgagg gccatggccc gcaagaacgc
     3781 tcccgagtcg ttcggatctt cgagctcgac accggaaacg aacgggtcgg cgaaggggtg
     3841 cgcaccgccg atgacgagcg agcggagccg ttccggcgcg tgcttcgcca tcccaaaacc
     3901 gatccacccg cccatcgagt agccgaagaa gtgggctcgc tcgacgtcga gcgcgtcgag
     3961 cacggccgcg acatcgccga cgcgaagttc gagcgtgtgc gacgtgcgct cgtgcggctt
     4021 gtcactttgg ccatggcccc gcgcgtcgag caaaatcaga cgatagcgtg agcgcagcgc
     4081 cctgacatag ccgtgttgaa cccacaccgc gccgttgctg ccgctcccgt ggtgcagcaa
     4141 gagcggtggc ccctcgccca tcaccgagta gtgaatgcga gttccggcgt gcaccaggtg
     4201 aggcatgagc gcgccgaagt tagcagcttt caatgacgac gcgaggcggc gtgcagccag
     4261 ggcgctggcg atgcttgcgc cgatctcata cgcgctgcac tacgtggccc cgagctctcg
     4321 ctgcttggct gcctcgtagc gctcggtaag cgcctgcgcc gactcgtctc cgagcgcggc
     4381 ttggccccgc ggaaagtagt caccctcttc gtcgaacacg tgctgctcga ccgtctgcgc
     4441 cagaagctcc atcgcacgct cccactcggg gtctgcgggc ttcagccggt cgagcctctc
     4501 gagcacggac tcgagctctc gaacctggtc tccgtgcgcc attgcgaggc gaatggtcga
     4561 ctcgtgttct gcgagcgcgc gatagacttc ggcgctctcg gcgcgttcgt gggcgaccaa
     4621 tcttcggcga agctcggcgt aaagccgcgc gcgctcgtcc gcgtccgagc agcgcctggc
     4681 tcgcttgacg agcgccgcaa gctcgccgtg ctcttgcatc aggtgtttga agacgccggt
     4741 caagccctgg aacgcggcct cactcgcctt gaccagctcc aacgccttct ccgtcgttcg
     4801 ttcccagacg ttcgccattg agctctcctg tctttcgttc gccgtcgcgc cgctcgtcat
     4861 tcgctcccag tgtgaggcga agctgcgcag gtcgtaccga ccttcccgcc accaggctcg
     4921 cgagcggtgt ggcagcacga tgtgtcccgc ttcgtcagcc ttgagcgtgt acattttgta
     4981 cgagctcgta cgagttccgc gcggcagtgg ggcgtcttcg cgtgcgctcg cgctcgtcgc
     5041 gaaggcttgc cctctctgcg cgacgatgaa aatgattcag cagacctacg cgcacgcaaa
     5101 ctctcctgcc aaactctcgt ggttcgcaga cgccacacct aagttgcaag atccggcccg
     5161 gagcgattcg tcccctggga accgatcggc acagcgcatg caacccagct cttggcagaa
     5221 gcaaactgac acgagcaacg cgtgaccgcg aatgtcgcca ccacgcggcg ccgaaaggac
     5281 gagacgacgg aggatcgaaa tggcgaacga aaccgaaagc tccgcgggcc ccgggaatca
     5341 cattttggga gcgctgcccc aagccgaaat tgatgcaatc gcgccccatc tcgagccgat
     5401 gttgctcgac cagcgtcacg ttgcgtacga cgtcaacact cccatcgagt acgtgtactt
     5461 cccagaagac tgcgtcatat cgctcgtcag cgtgatgcgc gatggacagg tcgtcgaagt
     5521 tgccaccgtg ggcaacgaag gaatgatcgg attgccgctc tttttgcggg ccaggcgcac
     5581 gctgggacaa gccttctgcc aagtacctgg cagcgctttc cgaatgaagg cggacgcctt
     5641 cgtcgagttc gccgcgaagg gcaagctcaa cgacttgatg caccgctaca cccaggcgct
     5701 gttcgcgcag gtgtcgcaaa gcgcagcctg taaccggctg cacctcgcgg aagagcgctt
     5761 ctcacgctgg attctgatga cccacgaccg cgtgcgcggc aatgacttcg cgctgaccca
     5821 agacttcctc tcgcagatgc tgggggtacg gagagcgacg gtctccgaag tcgcggcgca
     5881 agcgcagcgc gacgggctca tccgatatca ccgcgggcac atgacgatag tgaaccgcaa
     5941 gggactcgag gaacgcgctt gcgaatgcta ctcgataatc caggcggagt tcgagcagct
     6001 catcggagac agctcgaaga actcgctctc gcgcgctcgc gtttcgctgc cgagcttctc
     6061 acgggcgggc aaagcgaccg caggcgaggc gatcccgcac attgaccgaa cggaggctgg
     6121 ctcacccgct ccaactcctg ccgacaacaa cgcttcgacg taatctcgaa gttggcgcgt
     6181 tcgttgctgg tgtctcggcg tcgcaagctg cgaccagttg aacggaagaa tgagtcatga
     6241 ccaacaaact cggatggatc ggcctcgcgg cgttgggctt gtgcggagcg tgcgcttcgc
     6301 acgacagggc gcccgccgca tccgctgcga acacctccgc cgcgaacgtg gacgcagcca
     6361 ccacgaaccc caacacgggc cccaacttcg ccccggctcg cgcggatggc acggcctcgg
     6421 cgaccgcgac gccgggccgt gacacgcccc cgggtacgaa ccccgccccc ggaaccggcg
     6481 acacgatcgt cgatcgggat cgttcgatga cgggctctgc acgcaacgcg cgctcgacga
     6541 cgtccgccac ctcgtcttcg acggacacga gcgcgagctc ggatacgaac tcgggttacg
     6601 cgggctcgag cgacccggcg cagcgagctg acaccaacaa cggtccctcc gctaccagca
     6661 agaacaccga tacgtcggcg ccggacaata cgcgcgtcaa caagcgggac cgcaacgacg
     6721 cggctccgct ccccaccgat cagggcaaca acgaagcgga tctgaagacc acgcaggcga
     6781 tccgccaggc gttgatgaag gacaagaacc tgtctttcgg cgcgaagaac gtgaagatca
     6841 tcacgcagaa cggccgggtc accctgcgcg gcccggtgag caccgaagcc gagcgctccg
     6901 ccatcgaagc ggccgcgcgc aaggtcgcgg gcgccaacca ggtcgacaac caactcgaac
     6961 tgaagaacga ccaacgcaag tgagcgggcg ctggggctcg ccgtgctgtt tcaagcgagc
     7021 ggcacgtcga gccccacgat gcgcatcaag cggagcgcgt ttccggaagt gacgggcccc
     7081 gggtacaccc ggtagtcgaa cgt
//
LOCUS       DASTKS010000893         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1484413, whole genome shotgun sequence.
ACCESSION   DASTKS010000893 DASTKS010000000
VERSION     DASTKS010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484413"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VFQ35_18610"
     CDS             <1..1377
                     /locus_tag="VFQ35_18610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET9932726.1"
                     /translation="GALGPNGQPVRIVPLDSCIIQHPRVWEASGHVAGFSDPMVDCRE
                     TKARYRFDHLFVYVPANGDRQGKPLFCYLPDSPSETKQKKKAEKAWGEVASVPLSKIG
                     KEEWPSVLAPDAEKVGSLTEPRAFNLMFKTYVGATATEEDVAFLRPETAQGIFVQFKN
                     VIDTTRVRVPFGIAQTGKAFRNEVTPRNFTFRSREFEQMEIEFFCHESEARDWYAFWR
                     DTRVAWWKSLGLAGENLQLREHDKDELAHYARDGAGTSDVEYRFPFTAPGFGELEGIA
                     HRSNFDLSQHQKMAKTNLEYFDSERGEVLPNGSRKGEKYLPHVIEPSAGLDRGVLAVL
                     CEAFTEDASRPSPELLKIHPRLAPIKAAVFPLVNKDGMPEISEKLHAELTRRFFRSGF
                     IEHDAKAAIGKRYARMDEAGCPYCFTIDGETAAGQSVTVRDRDTGAQERIAIGRVGDW
                     LAEKLGAP"
     gene            1435..1617
                     /gene="yacG"
                     /locus_tag="VFQ35_18615"
     CDS             1435..1617
                     /gene="yacG"
                     /locus_tag="VFQ35_18615"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET9932727.1"
                     /translation="MRITCPTCKTVLEDVPDDYPPRPFCSARCKLADLHAWLNEEYKV
                     SEPLPLEPEDERRHLN"
     gene            1645..2508
                     /locus_tag="VFQ35_18620"
     CDS             1645..2508
                     /locus_tag="VFQ35_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9932728.1"
                     /translation="MSRLSRRSFFSLGVAWSASGFLRGPAYEEHELEIAESAPNLSRC
                     VVALPILRPKAAKLPIAILLHGLGETRDPKLGVRAWLDAYGLRSALDRLDEGHLRGDA
                     SYQYLSADERYALNDSLHREPFQGLCIVCPFLPNPHVGGNWQTSLLRYAIWATEKLLP
                     AVRREFPDVAGERVGLAGVSLGGFSALEVYLQRPTSFQALGSVQGAFSKVYARAAAQR
                     LLATGQKPAVYAGTSTFDPYRVANVDFARAVEAGGGNVKLNVRNGPHSQAWLREIGTL
                     DTLIWLDRALR"
     gene            2583..>2801
                     /locus_tag="VFQ35_18625"
     CDS             2583..>2801
                     /locus_tag="VFQ35_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET9932729.1"
                     /translation="MLRAITDDGAFRVITARTTETIRGALKAQGAKGSTARTFGDLIT
                     SAILFRETMAPQLRVQGVLRGANGSGNLV"
BASE COUNT          514 a          911 c          892 g          484 t
ORIGIN      
        1 ggtgccctcg gtccgaatgg gcagcccgtg cgtatcgtgc cgctcgacag ctgcatcatc
       61 caacacccac gggtttggga agccagcggt cacgtcgcgg gcttcagcga tccgatggtc
      121 gactgtcgcg agacgaaggc gcgctaccgc ttcgaccact tgttcgtgta cgtgcctgcg
      181 aacggcgacc gccaaggcaa gcccctcttt tgctacttgc ccgacagccc gagcgagacg
      241 aagcaaaaga agaaggccga gaaggcttgg ggcgaagtcg cgagcgtgcc gctctccaag
      301 atcggcaaag aggagtggcc ctcggttttg gctccagacg cggaaaaggt cggcagcctg
      361 accgagccgc gcgctttcaa tctgatgttc aagacgtacg tcggcgcgac cgcgaccgaa
      421 gaagacgttg cgttcctgcg tcctgagacg gcgcagggaa tcttcgtgca gttcaagaac
      481 gtgatcgaca cgacgcgcgt gcgggttccg ttcggcatcg cgcaaaccgg caaggctttc
      541 cgcaacgagg tcactccgcg caacttcacc ttccgtagcc gcgagttcga gcagatggaa
      601 atcgaattct tctgccacga gtcggaggcg cgcgactggt acgccttctg gcgcgacacc
      661 cgcgtcgctt ggtggaagag cctcggcctc gctggcgaga acctgcagct gcgcgagcac
      721 gacaaggacg agctcgccca ctacgcgcgc gacggagctg gtacgagcga cgtggagtac
      781 cgctttccgt tcacggcgcc gggtttcggc gagctcgaag gtatcgccca ccgctcgaac
      841 ttcgacctct cgcagcacca aaagatggcg aagaccaacc tcgaatactt cgattcggag
      901 cgtggcgagg tcttgccgaa cggctcgcgc aagggtgaga agtacctacc gcacgtcatc
      961 gaaccgtcgg cgggcctgga ccgcggcgtg ctcgcggtgc tgtgcgaggc cttcaccgaa
     1021 gacgcttcac gcccgagccc cgagctcttg aagattcacc cgcgcctcgc gccgatcaaa
     1081 gccgccgtgt tcccgctcgt gaacaaagac ggcatgcccg agatcagcga gaagctccac
     1141 gccgagctca cgcggcgctt cttccgcagc ggcttcatcg agcacgacgc caaggccgcg
     1201 atcgggaagc gctatgcacg catggatgag gcaggttgcc cctactgctt cacgatcgat
     1261 ggcgaaacgg ccgcaggcca atcggtcacc gtacgcgacc gcgatacggg cgctcaggaa
     1321 cgcatcgcca tcggtcgcgt gggtgattgg ctagccgaaa aactcggcgc gccctgaggc
     1381 ctcgacgagc tctctgcacg ggccgcatcg cgcgaaaagc gctatgctga gcccgtgcgg
     1441 ataacctgcc caacctgcaa gaccgtgctc gaagacgtgc cggacgacta cccgccgcgt
     1501 cctttttgtt ccgcccgctg caagctcgct gatttgcatg cgtggttgaa cgaagagtac
     1561 aaggtttctg agccgctccc gctcgagccc gaagacgaac gccgccatct gaactgaccg
     1621 cgccgctcgg gctacactcc gggcgtgtct cgcctctcac gccggtcgtt cttcagcctt
     1681 ggcgtcgctt ggtcggcatc ggggtttctg cgcgggcctg cgtacgaaga gcacgagctc
     1741 gaaatagccg agtcggcgcc gaacctctcg cgctgcgtgg ttgcgctgcc gattctccgc
     1801 ccaaaggcgg caaaacttcc gatcgcaatc ctgctgcacg ggctcggtga aacgcgcgat
     1861 cccaagctcg gcgtgcgcgc gtggctcgac gcctacggat tgcgttccgc gctcgatcgc
     1921 ctggacgagg ggcacttgcg cggggatgcg tcgtaccagt acttgagcgc ggacgagcgc
     1981 tacgccctga acgactcgct gcatcgagag ccgtttcagg ggttgtgcat cgtgtgcccc
     2041 ttcttgccca acccgcacgt gggtgggaat tggcaaacgt cgttgttgcg ctacgcgatt
     2101 tgggcgacgg agaagctgtt gccggcggtg cgccgcgaat tccccgacgt cgcgggagaa
     2161 agggtggggc tcgccggcgt gtcactcggt ggattcagcg cgctcgaagt ctatttgcag
     2221 cgtccgacgt cgttccaagc gctcggctcc gttcaggggg cgttcagcaa agtttacgcg
     2281 cgggctgccg cgcaacgtct gctcgcgact ggccaaaagc cagccgttta cgcggggact
     2341 tcgacgttcg atccgtatcg tgtcgctaac gtcgacttcg ctcgcgcggt cgaagcgggt
     2401 ggcggcaacg tgaagctcaa cgtgcgtaac ggtccccaca gccaagcctg gctccgggaa
     2461 atcggcacgt tggacacttt aatctggctc gaccgcgcgc tgcgttaacg accgcgcaac
     2521 cgaacctggt ggaaccgcgt tccgcgtcgt atgctgtgga ggtgacgcaa acgaaggaca
     2581 gcgtgctgcg cgccatcacc gacgacggcg catttcgggt gatcaccgcg cgaaccaccg
     2641 agacgatccg gggcgcgctc aaggcccaag gggccaaagg aagcaccgcg cgaaccttcg
     2701 gcgatctcat caccagcgcc atcctcttcc gcgagacaat ggcccctcag ctccgggtcc
     2761 aaggtgtcct gcgcggcgcg aacggctcgg gcaacctcgt g
//
LOCUS       DASTKS010000894         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_366447, whole genome shotgun sequence.
ACCESSION   DASTKS010000894 DASTKS010000000
VERSION     DASTKS010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366447"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFQ35_18630"
     CDS             complement(<1..729)
                     /locus_tag="VFQ35_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932730.1"
                     /translation="MQLKCAGSVGAPSLALDAESSELGFSAYSKSANAWQFEVAERKP
                     DELRRAVAKGIELGWVFATPEGYRHIGSAGTSLSWLGDDFGPSEPIAVAGGSAIQDVW
                     VDANGVAHFLVSRTDGLNVESSDGTSHHIADRLLQYQRLTPTTPVGVIAAEQGQFTSE
                     TDTTFRLLGWELGGEAKVLASVEGEAPVATFAQAEGSRAITFDTYGPRTNVPNGPMVA
                     SARRLLFVNEDGSVGHAWEGTENSL"
     gene            1691..>2596
                     /locus_tag="VFQ35_18635"
     CDS             1691..>2596
                     /locus_tag="VFQ35_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932731.1"
                     /translation="MTLESTLSSMLQGSGIQQTLFETLAAPPTDGSLSVGPEQALAAF
                     TVPIDGAQLSDLAGRTNQFSTENVGAAFSRLAERATPLVASLPGVGDVLGPLNGALAT
                     LEQLSTQDVAATLRDLASRVGAEFDGSTEGGLSGRLLRIATLLSEAGAGTSLLDLLGQ
                     LLRPTGLDLPRLPVTETFRVVDGTARAFGGLMMLETVLAEAERLSGILSRQLDAAHIT
                     AQIDGLEAALSLGPVTLADFVTSVAPNDPATVAAATNAVLAFATSLDALRDDVAAGFG
                     LGEATLVYMKPEVILAETELALSLMR"
BASE COUNT          429 a          845 c          811 g          511 t
ORIGIN      
        1 gagcgaattc tcggttccct cccaagcgtg gccaacgctg ccgtcttcgt tcacgaacaa
       61 cagccgtcgc gcggacgcta ccatcggacc attcggaacg ttggtcctcg gcccgtaggt
      121 gtcgaaagtg atcgctcgcg atccctccgc ctgtgcaaac gtcgcaaccg gcgcctctcc
      181 ttctacgctg gcgaggactt tcgcctctcc cccgagctcc cagccgagta ggcgaaaggt
      241 cgtatccgtt tcgctcgtga attgcccttg ttctgcggca atgacgccaa cgggtgtggt
      301 cggcgtgagg cgctggtatt gcagcaagcg atcagcgatg tgatggctcg ttccgtccga
      361 agattcgacg ttcagcccgt ccgtgcgcga cacgagaaag tgcgccaccc catttgcgtc
      421 gacccagacg tcttgaatcg cgctacctcc cgcgaccgca ataggctcgc tcgggccgaa
      481 atcgtcgccg agccaactca ggctcgtccc cgcggagccg atgtggcggt atccttcggg
      541 ggttgcgaat acccagccga gctcgattcc cttcgcgacg gctcgtctga gttcatctgg
      601 cttgcgctcg gcgacttcga actgccaagc gttcgcgctt ttggagtacg cgctgaagcc
      661 gagctctgaa gactccgcat cgagcgccaa gctcggggct ccgacgctcc ccgcgcattt
      721 gagttgcacg accgagctcg caaggcacgg cgaagtcaca caaaacgccg cggggttact
      781 ctccgtcgcg gtacacggac tcgccacgat tcccgcatcg ttgccgcctg tgctcgtcgg
      841 ggttgctccg cccagtcccg agtcggcgcc cgctgagtga gctccgccgc tgaacgccga
      901 agcgcccccg gaagcgaacc ctgacgctcc cgcgctcgcg tcccctccgc ccgctgtcga
      961 cgcttctcta gattctgcgc gaccgcatgc gacgacggca actgcagcga gtatccaacg
     1021 atagagcccg agttgcggca tgcggccctc tccagcaagg gctgtgcccg gtagttcctg
     1081 aatgattgcg taggagacac ggcactcctg tgccaattgg cacaacctct cgcgatctag
     1141 gatagtcccc gcgacggatg gcagcggaac gcgatgagct agcggctgag gtcaatcgcg
     1201 cgcgcccatt caaagcgaga gagtccacgt tctatgtgct tgcgcggatg taggctctag
     1261 tcgcctgtga cttcgctgac tgttcaggtg agctccgggc ggttggcggg tccgcgcttg
     1321 cgcgcagatg tcgaaagcca ccagcctcgc tacttgcggg agtgagcgat cgcgatcgca
     1381 cgagttcagg cccgaggcag tgtggacctt catctcgaaa tagctattcg tccagtacgt
     1441 ggactgtatc tgtgcactat gacggtgtaa cgacaccgcg ggtgaggagc tccggtcttg
     1501 cgacctcacc ctggtcccgt ctcgttggtg tctactgtgg acaagtgtga ttatcgcatc
     1561 ttgccgatcc gagcaacgca atgactatgc aagtctagct gaacgcggtt aatgttggcg
     1621 cgggctgcgg cgctcggttg cgaacgcaga gcttggattg ggttcgttcg ttgtcgcggc
     1681 ggatacctgc atgactcttg agtccacgct gagctcgatg ctgcaaggga gtggtattca
     1741 gcaaacgttg ttcgagacgc tcgccgctcc gccgactgac ggctctctca gcgtcgggcc
     1801 agagcaggct ctcgcggcgt ttaccgtccc gatcgacggc gcacaactga gcgatctcgc
     1861 ggggcgcacg aaccagtttt cgacggaaaa cgtcggcgca gcgttttcgc gactggcaga
     1921 gcgagcaacg cccttggtcg cgtcgctccc gggcgtgggc gacgtgctcg gacccctcaa
     1981 cggcgcactg gcgacgctcg agcaattgag cacgcaggac gtagccgcga ctctgcgcga
     2041 cctcgcttca cgcgtgggag ccgagttcga cggttccacc gaaggcggcc tttctgggcg
     2101 tctcctccgc atcgcgaccc tgctgtcgga agccggcgcg gggacgagct tgctcgatct
     2161 gttgggtcag ttgttgcgac ccaccgggtt ggatctgccc agacttccgg tcacggaaac
     2221 gttccgggtc gtcgacggga ccgcgcgcgc gttcggcggc ctgatgatgc tcgagacggt
     2281 gctcgccgag gccgagcgtt tgagcggtat cttgtctcgt cagctcgacg cggcccacat
     2341 cacggcacaa atcgacggcc tcgaagcggc gctttcgctc gggccggtca ccctcgccga
     2401 cttcgttacg agcgtcgccc cgaacgaccc ggcgaccgtc gcggcggcga cgaacgcggt
     2461 gcttgcattc gctacgtcgc tcgacgctct gcgcgacgac gtggccgctg ggttcggcct
     2521 cggggaagcc accctcgttt acatgaagcc ggaggtcatt ctggcagaaa ccgagctcgc
     2581 gctcagtttg atgcgc
//
LOCUS       DASTKS010000895         5852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1931773, whole genome shotgun sequence.
ACCESSION   DASTKS010000895 DASTKS010000000
VERSION     DASTKS010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931773"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFQ35_18640"
     CDS             complement(<1..572)
                     /locus_tag="VFQ35_18640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932732.1"
                     /translation="MKAKLLLIDDSEAQSGQIKAVLERLGYSVECANSGVEGLRLART
                     IQPDLVLLDVVMTDIDGFAVCRWLKMNADTREIPVIMLTVRTALADRVEGLNIGADDY
                     LSKPFQDEELEARIFAALRVKAAHAELKERNHQLETMLHHVEALAITDPLTGLFNRRR
                     FTDVLKREFAVTKRYKNTLSCLMIDLDHFKR"
     gene            1086..2792
                     /locus_tag="VFQ35_18645"
     CDS             1086..2792
                     /locus_tag="VFQ35_18645"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9932733.1"
                     /translation="MASDAIVTELGAFVALGESNRIRVQKQAVQGQPFHEALRVEVAE
                     AATRPWDLQLQTKTERAVEKGDVLLATFFVRAEWSPEESGEVLTEFVFERGADPWTKS
                     ASFEVRAGREWKKVFVPFVAAESYAAGEAHVNFRVGFGAQQLEIGGVVVENFGKRLSL
                     ADLPVIKISYPGMEPDAAWRAPAAERIEKIRKAPLRVVIKDKSGNPIPNADVLVRTTK
                     PAFALGTCVPAALITQPGNETFKRVTTELFTIATLENDLKWQPLAGNWGGGYTLERAR
                     AGVAWLRERGLDVRGHVLVWPGWRNLPSSLRAFEKDPPRLRAEVKRHVAELVTAMKGT
                     LAHWDVVNEPFDNHDILDILGPEVMVDWFKEARAHDPSAKLFINDYAILSGGAGTTPH
                     RDAYEKTIQMLVEHDAPFDGIGMQGHFGTSLTGPEDLLKILDRYAKFGKTIYVTEYDV
                     VIDDESVAADYTRDFYTTLFSHPAVGGIVMWGFWDGSHWKHNAPLYRNDFSLKPAGEV
                     VRDFALKSWRTNATGKTDAAGAFALRAFLGELEVVVSAGSSKKVVKATLANSGREVVV
                     TI"
     gene            complement(2782..3774)
                     /locus_tag="VFQ35_18650"
     CDS             complement(2782..3774)
                     /locus_tag="VFQ35_18650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9932734.1"
                     /translation="MSRLTALGLALLAVACGESGHSEVRARVTFVGDVLLDSAPGNTI
                     RDGGDPFASVAQVLTDSDLSIANLECSVATTGERAYKEYTFRADPRTLTLLKRYVGVV
                     SLANNHSGDYGPAALTETMKHLTERNIRFFGAGKDSQDAHSPLLVQARGVKLGLLGYD
                     EYQPRWFEAGPNRPGVAWSEDEQVVDDIRAARARGAAVVIPFMHWGWEEEDEPTPRQR
                     ALAHRMIDAGADAVVGSHPHRVQGAEIYRDRPIVYSLGNFVFDLTESDIERLGWLLRV
                     ELDERGVRRWSTLLVRIDSRGSPALDRSAWTPCGTRGRVRVGRCLGGDWLSARW"
     gene            complement(3771..4184)
                     /locus_tag="VFQ35_18655"
     CDS             complement(3771..4184)
                     /locus_tag="VFQ35_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932735.1"
                     /translation="MSSRFTPFRWLVRSWLLSYLAIPACAPHKAPQGARCTLKYAVTT
                     RELEVRPTDAPYEAPLVLVGERFGFKAVLRDGERASLNLYVYDREHDTLLQEVKYTAP
                     FPGSRADAEFGFTGHQYVYSSSGREFEYWCRWSSP"
     gene            complement(4293..5045)
                     /locus_tag="VFQ35_18660"
     CDS             complement(4293..5045)
                     /locus_tag="VFQ35_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932736.1"
                     /translation="MRGLQLIFGFLFVCACSSSNDDKSRTREEFCADWAKAACTTQTV
                     SACQATSAEACHLKQQAFCLGLVPADFSDARGDACINAVGAAYKDADLTQAELSTVLE
                     LGEPCNAIVRGPKDVGEQCSTDRDCDGTKGVRCVFHGAATTGTCQVAQEVGAGQRCSA
                     LAQTCGEGFYCDGNNCIAVKETGDDCTTSYECGSLGLCNDSVCAPKLAVGATCEDANQ
                     CASGICYSVNGQKTCADRIRLSPAESICENLR"
     gene            complement(5169..5624)
                     /locus_tag="VFQ35_18665"
     CDS             complement(5169..5624)
                     /locus_tag="VFQ35_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932737.1"
                     /translation="MSFGVGTQISPPPPGCFKGTFLCAKGSIPASDTAGAGIGWNLAQ
                     PRASLSANVLALTGAIKITFVGAKTGTRVALGPSSGDDYCYYLTAAEVSAGAATIPVS
                     SFKSACWDPVNARPYAGEATIRSIQLMVVGSTSGGPATFDFCITDVEPA"
BASE COUNT         1086 a         1880 c         1839 g         1047 t
ORIGIN      
        1 cgcttgaagt gatccaggtc gatcatcaag cagctcagcg tgttcttgta acgcttggtc
       61 accgcgaact cgcgcttcag cacgtcggtg aagcggcggc ggttgaagag tcccgtcagc
      121 gggtccgtga tcgccagcgc ctcgacgtgg tggagcatcg tctcgagctg atggttccgc
      181 tccttcagct cggcatgcgc ggctttgacg cgcagcgcgg cgaagatgcg cgcctcgagc
      241 tcttcgtctt ggaacggctt cgaaaggtag tcgtccgcgc cgatgttcaa gccctcgacc
      301 cgatcggcga gcgccgtgcg cacggtcagc atgatgaccg ggatctcgcg cgtgtcggcg
      361 ttcatcttca gccagcggca aacggcgaag ccgtcgatgt ccgtcatcac gacgtcgagc
      421 aggaccaagt ccggttgaat ggtgcgcgcc aggcgaagcc cttcgacgcc cgagttcgcg
      481 cactctacgg agtagccgag cctttccagc actgccttta tttggccact ctgggcttcg
      541 ctatcgtcga ttaagagcag cttggctttc acggatgact cattccccga tgtctcggcg
      601 cacgagcgag cttgagcgtc tcagcatacg gatgaccttt tgacgatacg tcccttgcta
      661 tctgatgccg agtaaaacgg tcacggccgg cgtcggccct tggggagtcg ggcgggtgaa
      721 aagtgcgcca atgtagtcgg tcgctctcgc ggttttcggc tacgtccctg tcaacggtca
      781 gtcgtggaag tcagtaacgg gaaaaatggt ttgtacgagc gcggccgtat ggccaggcgc
      841 gccgggatac cgacgaaccc gttgatgttt gcgccttggt cggctctagc tccgccgggt
      901 tactgtacgc tttcggcatg ttcatgcgcc tcgaaactga gcgagcgctg gtcctgagcg
      961 cgctcgtgct ttcactgttc ggttgcgcga agcccgagtg cagctcggtg gcggggcctt
     1021 cgacatcggc gcatgtttcc tcacctacag cgcaaactac ggctgcgact gggagatatg
     1081 ggcaagtggc gagcgacgca atcgtgaccg agctcggagc tttcgtggca ctcggggaat
     1141 ccaaccggat ccgcgtccaa aagcaggcgg tccagggaca accctttcac gaagcgctcc
     1201 gcgtggaggt ggccgaggcg gccacgcgcc cctgggacct tcagcttcaa acgaagaccg
     1261 agcgcgcggt ggagaagggc gacgtgttgc tcgcgacatt cttcgtgcgc gccgagtggt
     1321 ctccggaaga gagcggcgaa gtcctgactg aattcgtgtt cgaacgcggg gcggatccgt
     1381 ggacgaagtc ggcgagcttc gaggtgaggg cagggcgcga gtggaaaaaa gtgttcgtcc
     1441 cgttcgtggc cgcggagagc tacgcggccg gggaagcgca cgtcaacttt cgcgtcgggt
     1501 tcggcgctca gcaactcgag atcggtggag tggtggtgga gaacttcggc aagcgcctct
     1561 cgctcgccga cttgccggtg atcaagatca gctacccggg gatggagccg gacgcggcct
     1621 ggcgcgcgcc cgcggccgag cgcatcgaaa agattcgaaa agcgccgctt cgagttgtca
     1681 tcaaggacaa gtcgggcaac ccgattccca acgcggacgt actggtccgc acgaccaagc
     1741 cggcctttgc gctcgggact tgcgtacccg cggcgttgat cacgcagccc ggaaacgaaa
     1801 ccttcaaacg agtcacgacc gagctcttca cgatcgccac cctcgaaaac gacttgaagt
     1861 ggcagccgct cgccggcaat tggggcgggg gatacacgct cgaacgcgcc cgcgccggcg
     1921 tcgcttggct gcgcgagcgc ggcctcgacg tgagaggtca cgtgctcgtc tggcccggct
     1981 ggcggaacct cccatcctcg ctccgtgcct tcgagaaaga cccgccgcga ctgcgcgccg
     2041 aggtgaagcg gcacgtcgcc gagctcgtga ccgcgatgaa aggcactctc gcgcactggg
     2101 acgtcgtgaa cgagccgttc gacaaccacg acatcttgga catcttgggt ccggaagtga
     2161 tggtcgactg gttcaaagaa gcgcgcgcgc acgacccgag cgcgaagctc ttcatcaacg
     2221 actacgcgat cctgtcgggc ggcgccggca cgacgcccca tcgcgacgcc tacgagaaga
     2281 ccatccagat gctcgtcgag cacgacgcgc ccttcgacgg catcggcatg caaggccact
     2341 tcggtacgtc gctgacgggg ccggaagact tgctgaaaat cctcgatcgc tacgccaagt
     2401 tcggcaaaac catctacgtc accgagtacg acgtcgtgat cgatgacgag tcggtagcgg
     2461 cagactacac gcgcgacttc tacaccacgc tgttctcgca tccggcggtg ggcggcatcg
     2521 tcatgtgggg cttctgggac ggcagccact ggaagcacaa cgcgccgctc taccggaacg
     2581 acttttcgct caagcccgcc ggcgaagtag tgcgcgactt tgccttgaag tcgtggcgca
     2641 ccaacgcgac cggtaagacg gatgcggccg gagcgttcgc gctgcgcgct ttccttgggg
     2701 agctcgaggt cgtggtgagc gcgggttcgt cgaagaaggt cgtcaaagcg acgcttgcga
     2761 actccgggcg cgaagtcgtc gtcaccatct agcggagagc cagtcgccgc ccaagcaacg
     2821 tccgacgcga acgcgtccgc gggtgccgca cggggtccag gcgctccggt cgagagccgg
     2881 acttccccga gaatcgattc ggacgagcag cgtcgaccag cgccgaacgc cacgttcgtc
     2941 gagctccact cgaagcagcc aaccgagtcg ttcgatatcg ctttcggtca agtcgaagac
     3001 gaagttgcca aggctgtaga cgatggggcg atcgcgataa atctcggcgc cctggacccg
     3061 atgcggatgg gagccgacca ccgcgtccgc gccggcgtcg atcatccgat gcgccaacgc
     3121 gcgctggcgc ggcgtcggct cgtcttcctc ttcccaaccc caatgcatga aagggatgac
     3181 cacggccgcg cctcgagcgc gcgccgcgcg gatatcgtcg acgacttgct cgtcctcgct
     3241 ccaagccact ccgggtcgat tgggcccggc ttcgaaccaa cgcggctgat actcgtcgta
     3301 accgagcagc ccgagcttca ccccgcgcgc ttgcacgagg agtggtgaat gtgcatcttg
     3361 cgagtctttg cccgcaccaa agaaacggat gttacgctcg gtcaggtgct tcatcgtttc
     3421 ggtcaacgcg gcaggcccgt agtcgcccga gtgattgttc gcgagtgaaa ccacgccgac
     3481 gtaacgtttc aggagcgtga gcgtgcgcgg atccgcgcga aaggtgtact ccttgtaggc
     3541 tcgctcgccc gtcgtcgcga cggaacattc gaggttcgca atgctgaggt ccgagtcggt
     3601 caacacctga gcgacggacg caaaaggatc gccgccgtcg cgtatcgtgt tacccggtgc
     3661 cgagtcgagc agcacatctc cgacgaacgt aacgcgtgcc cgcacctctg aatgaccgct
     3721 ctcgccgcat gcgaccgcga gcagcgcgag cccgagcgcg gttagacggc tcacggtgag
     3781 ctccacctgc accaatactc gaactctcgc ccggacgacg agtacacgta ttgatgcccc
     3841 gtgaacccga actcggcatc ggcgcggctc ccgggaaagg gcgccgtata cttcacctcc
     3901 tgaaggagcg tgtcgtgctc gcgatcgtac acatacaggt tcagcgacgc acgctcgccg
     3961 tcgcgcagca ccgccttgaa gccgaaccgc tcgccaacga gtacgagggg cgcttcgtaa
     4021 ggcgcgtccg ttggtcgcac ctcgagctcg cgcgtcgtga ccgcatattt cagcgtgcac
     4081 cgcgcgccct gcggcgcctt gtggggcgcg caggcaggga ttgcaaggta cgacagtaac
     4141 cacgaacgaa ccaaccagcg gaacggcgtg aagcgcgaag acaagcggcc aaaagtcaac
     4201 cacgcagcca gtggggtgac tagggcgcac cttgatcccc gcgcttccga gcggtttttg
     4261 cgccgcactg aggcggggct gtctgtctca gctcagcgca gattttcgca aatggactcc
     4321 gcgggcgaca ggcggattcg gtcggcacac gtcttctgcc cattcacgct gtagcaaatc
     4381 cccgaagcgc actggttcgc gtcctcacag gtcgcgccga cggcgagctt cggcgcgcag
     4441 accgagtcgt tgcacagccc gaggctgccg cattcatagc tcgtcgtgca atcgtcccca
     4501 gtttccttta cggcgatgca gttgttccca tcgcagtaaa agccctcgcc gcaggtttgc
     4561 gcgagcgcgc tgcagcgctg acccgcaccc acctcttgcg ccacctggca cgtccccgta
     4621 gtggctgcgc cgtggaatac acagcggacg cctttcgtcc cgtcgcaatc gcgatccgtc
     4681 gagcactgct caccgacgtc cttgggcccg cgcacgatag cgttacacgg ctctccgagc
     4741 tccagcaccg tagagagctc ggcttgcgtc aaatccgcgt ctttgtaggc ggcacccacg
     4801 gcgttgatgc aggcgtcgcc gcgcgcatcc gagaagtccg cgggcacgag cccgaggcaa
     4861 aacgcctgtt gcttcaggtg gcaagcctcg gcactcgtcg cttggcaggc cgaaaccgtc
     4921 tgcgtcgtgc acgccgcttt cgcccagtcc gcgcaaaact cttcgcgagt cctcgattta
     4981 tcgtcgttgc tgctcgaaca agcacatacg aagagaaagc cgaatatcaa ttgtaagccg
     5041 cgcatgcgcg gcgaactgca agacacgggc cgcttgcggc cgagcgtttc ggcttcgaca
     5101 aatgcgctac ggtggggcgg gctgggcggc ggtggtaccg tcgaaggaca gctaacccac
     5161 tggcgccttc aagcgggctc cacgtctgtg atgcagaagt cgaatgtcgc ggggccgccg
     5221 ctcgttgagc ccacgaccat gagttgaatt gatctaatcg tcgcctcccc cgcatagggc
     5281 cgcgcattca cgggatccca acacgcggac ttgaaactcg aaacgggaat ggtcgccgcc
     5341 cccgcgctca cttccgccgc agtcaagtag tagcaataat cgtcgccgct cgacggcccc
     5401 agagcgaccc tcgttcccgt cttcgcgccc acgaaggtga tcttgatagc gccggtgaga
     5461 gcgagaacgt ttgcgctcag cgaggcacgc ggctgtgcga gattccaacc aatgccggct
     5521 cctgccgtat cagatgccgg gatcgagccc ttcgcacaga ggaaagtgcc cttgaagcat
     5581 cctggcggtg gtggcgagat ttgggtaccg acgccgaagc tcaacgtgta tgcgaatcca
     5641 gtccatggtg cgcttcctcg agaggtgcca aacccgtcga tgaagatcgc actctctttt
     5701 gctgtgagcc cgctatacgt ttcacaattc gaagagccgc cgcttgtcgc cgaccccgct
     5761 gtggcgacgg aaccgcctgt tgagagtgcg cctcccgtcg cggtcgagac tcccccaacg
     5821 cccgctacgt tgacgcctcc cgcaacagcg at
//
LOCUS       DASTKS010000896         5457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1931896, whole genome shotgun sequence.
ACCESSION   DASTKS010000896 DASTKS010000000
VERSION     DASTKS010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931896"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFQ35_18670"
     CDS             complement(<1..206)
                     /locus_tag="VFQ35_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET9932738.1"
                     /translation="MTYPEHVPIAILGAGLTGLSCALALAGRAPYFIAEKSSRAGGVA
                     STTEERGYRFDHTGHLLHLRSAALR"
     gene            318..1097
                     /locus_tag="VFQ35_18675"
     CDS             318..1097
                     /locus_tag="VFQ35_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932739.1"
                     /translation="MTLRPRNMLGSSDWLVTAVKAAFYATLGLFAHGCASAASGGAHA
                     DVPAPNQVVSSPPGPQRASPLPVSSRLGSKNYRCNLVVGPPAATQWFDAGFEQLVSND
                     AWEALSTPDAFVQNLGDPGARAWSAPVRSPCSERAEAPERVVLFVVENKRATQDDWLT
                     AVTAAVNAVRARYPTAREIQLVPAIQGPSESPCPNEPGDAALSAHAIERVTQQFDGLV
                     QAGPKVELDRCDQFGSQRLELTPAGNTRAAQLFAQYYSGDS"
     gene            complement(1105..3852)
                     /gene="polA"
                     /locus_tag="VFQ35_18680"
     CDS             complement(1105..3852)
                     /gene="polA"
                     /locus_tag="VFQ35_18680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET9932740.1"
                     /translation="MATELFPAGSPDVLYLVDLSSYVLRAYHAVAPLSSPNGEPTHAV
                     HGTVSMLERLLRERRPALFAIAMDSGRATFRKEIYDAYKANRPAAPEDLRTQLARLEQ
                     IVRAFQVPILKQDGVEADDLIASCVKCASARDIRSVIVAADKDLMQLVSDRVVMYDTM
                     RDKVFGVPEVEERFGVRVDQVRDYLALTGDSSDNIPGVPSVGPKTAKELLNQFGTLEG
                     IYQNLDKIPRKGLRDTLTNNRDNAFLSQRLVTLKDDCQAEFDPENFGRRERDVATLRE
                     LYSELGFTRQLTQLENEPAKPSPSTRPSAKPAAAEVPPAPAQLATHHLVRTAEELRTL
                     VETARASGRLALEAFTDQPSPTRGRLVGLALAMDAEKAYYVPIAHRHLSSGPQLALGI
                     VRELLGPTLADEKIQKLGIDLKLSTVALTRAGIPVAGFGFDATLASYLFDPEGRHDRN
                     SLAQRELGIVASTYDQLTKPGRGPRALLDELPPEDVLVYAAQSAEFAFKLEARMAPRV
                     NEEGLEKLYREVELPLAAVLTELELAGVLVDTSGLADIGKDCERELERLEKQAHEVAG
                     HPFNVNSPRQLETLLFDEVGLKPIKRTKTSRSTDAATLEALADQHELPRIILEIRQLA
                     KLKGTYIDALPTLVDKATGRIHTSWEQAVAATGRLSSSDPNLQNIPIRSELGRKIRAT
                     FVAPPGTLLVSADYSQIELRVLAHLSQDPVLLDAFRTGQDIHTRTAMEIFEVDAEHVT
                     REHRTRAKAVNFGVIYGQGDSGLAKVLGIPRAEASSFIAAYFRRYQGVRRFMNETLDR
                     ARSGEAVTSILGRRRLLPNIGSANRAERLAAERIAMNMPIQGTAADILKLAMLALKKP
                     VTPGARMVLTVHDELVFEVPEAEVEEATEAIRTKMETAFPLDVPLVVDVGSGPNWNAA
                     H"
     gene            3998..4930
                     /locus_tag="VFQ35_18685"
     CDS             3998..4930
                     /locus_tag="VFQ35_18685"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932741.1"
                     /translation="MHLAALDLGSNSFHLLVAHVGSTGAITKLASHKEVLKLGRVVQE
                     AGRLPELEYANALDAVAKLTSIARAFRAERLVVAATSALRDAANGREFCREVRERFGV
                     TVDLLSGAEEGRVVYRGARSAFPGLTGRVGVVDIGGGSVEVCLGEGNACQHVASLPLG
                     FLKVAAKLDGAAGRGDRLRALVLEETQELRDRLRWQRPETWLFSGGTARAIGKLVVAG
                     PAGISQGAVRRVAEELAQASPERLRALGVDAKRIDGIAAGALVFEALVDAFSVKSLRV
                     SPGGLREGLLLRELGNMGQPLETRDFGLEWASAG"
     gene            5104..>5457
                     /locus_tag="VFQ35_18690"
     CDS             5104..>5457
                     /locus_tag="VFQ35_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9932742.1"
                     /translation="MAENDPPRTLVAGKYQLSRLLGRGGMGSVWEGVHTTLGTRVAVK
                     FIEAEYANSTEARSRFENEARAAAKLQSKYVVEVYDHGVSEDGRPFIVMEYLHGEPLD
                     RRLDRVGRLPAKETAF"
BASE COUNT          839 a         1698 c         1900 g         1020 t
ORIGIN      
        1 cgcagcgccg ccgaacgaag atgcaagagg tgccccgtgt gatcgaacct gtagccgcgc
       61 tcctcggtcg tgctcgcgac gccgccagcg cgcgaagatt tctctgcgat gaaataggga
      121 gcacgccccg cgagtgcgag cgcgcaagac agacccgtga gccccgcgcc caagatggct
      181 atcggaacgt gctcgggata ggtcatgtcg agaagggcgc gttctcccgc gcttgggcgc
      241 ttcctctagc acgcttcacg cttgcattcg tggctcgatt cgggtctctt cgtcgagacg
      301 gcgcggcgct cgcgcccttg acgcttcggc cgcgcaatat gctcggaagc tcggactggc
      361 tcgttacggc ggtgaaggcc gcgttctacg cgacattggg tctctttgcg cacggctgtg
      421 cgagcgccgc ttcgggtggc gcgcatgccg acgtgcccgc tccgaatcag gtcgtttcgt
      481 cgccgcctgg cccccagcgc gcgtcgccgc tcccggtgag ttcgcggctc ggcagcaaga
      541 actatcgctg caatttggtg gtcggcccgc ccgcggcaac ccaatggttc gatgctggtt
      601 tcgagcagct cgtatcgaac gacgcttggg aggcgctcag cacccccgac gcgttcgtgc
      661 agaacttggg tgaccccggc gcccgcgcct ggtcggcgcc cgtgcgctcg ccgtgctccg
      721 aacgcgcaga ggcgccggag cgcgtcgtgc tcttcgtggt cgaaaacaag cgcgcgaccc
      781 aagacgactg gctcaccgcc gtgactgcgg ccgtgaacgc tgtgcgcgcc cgctatccga
      841 ccgcgcgaga gatccagctc gtcccagcga tccaggggcc gagcgagtcg ccctgtccga
      901 acgagccggg ggacgccgcg ctgtcggcgc acgccatcga gcgcgtgacc caacaattcg
      961 acggcctcgt acaggcgggg cccaaagtcg agctcgaccg ctgtgaccaa ttcggcagcc
     1021 agcggctcga gctgacccct gcaggcaaca ccagggcggc gcagctcttc gcccagtact
     1081 actcaggcga ctcgtagcaa tccatcaatg tgccgcgttc cagttggggc cggagcccac
     1141 gtcgacgacg aggggcacgt cgaggggaaa cgccgtttcc attttggttc ggatcgcctc
     1201 agtggcttcc tcgacctcgg cctccggtac ctcgaagacg agctcgtcgt gtacggttag
     1261 aaccatgcgg gccccggggg taaccggctt tttcagcgcg agcatcgcga gcttcaagat
     1321 atccgccgcc gtgccttgaa tcggcatgtt catggcgatg cgctctgccg cgagtcgctc
     1381 cgcgcggttg gcgctcccga tgttgggcag aagccggcgc cggccgagga tgctcgtcac
     1441 cgcttcgccg ctccgcgctc ggtcgagcgt ctcgttcatg aagcgccgca cgccttggta
     1501 gcgtctgaaa taggcggcga tgaagctgct tgcttcggcg cgtggaatgc cgagcacctt
     1561 ggcgagaccg ctgtcgccct gaccgtagat gacgccgaag ttgacggctt tcgcgcgcgt
     1621 ccggtgctca cgcgttacgt gctcggcgtc gacttcgaag atctccatcg cggtgcgagt
     1681 gtggatgtcc tgcccggtgc ggaaggcatc gagcaatacc gggtcttggg agaggtgcgc
     1741 cagtacgcgc agctcgattt gcgaatagtc ggcgctcacg agcaacgtcc cgggtggcgc
     1801 gacgaacgtc gcgcggatct tccgcccgag ctcgctgcga atcgggatgt tctggaggtt
     1861 cggatcgctc gacgagagcc ggcccgtagc cgcgacggct tgttcccacg acgtgtgaat
     1921 tcgcccggtg gccttgtcga cgagagtcgg gagcgcgtcg atgtaagttc ccttgagctt
     1981 cgcgagctga cggatttcga ggatgatgcg cgggagctcg tgttggtcgg cgagcgcctc
     2041 gagggtcgca gcgtcggtgg agcgtgatgt cttggtgcgt ttgatcggct tcaagccgac
     2101 ttcatcgaac aacagcgttt cgagttggcg cggcgagttg acgttgaagg gatgccccgc
     2161 cacctcgtgc gcctgctttt cgaggcgctc gagttctcgc tcgcagtctt tcccgatgtc
     2221 ggcgagccca ctcgtgtcga cgagcacgcc ggcgagctcg agttcggtca agaccgcggc
     2281 gagcggcaac tcgacctctc gatagagctt ttcgaggcct tcctcgttca cgcgcggcgc
     2341 catgcgcgcc tcgagcttga aggcgaactc ggcgctttga gcggcgtaca cgagcacgtc
     2401 ttccggcggt agctcgtcga gcaacgcgcg cggtccgcgt ccgggtttcg tcagctgatc
     2461 gtaagtgctc gccacgatcc cgagttcacg ctgggcgagt gagttgcgat cgtgtcggcc
     2521 ttcgggatcg aagagatagc tcgcgagcgt cgcgtcgaag ccgaacccgg cgaccgggat
     2581 cccggctcgc gtcagcgcga ccgtgctcag cttgagatcg atcccgagct tctggatctt
     2641 ctcgtcggcg agggtcgggc cgaggagctc tcgcacgatc ccgagcgcga gctgcgggcc
     2701 gctgctcagg tgccggtgcg cgatgggcac gtagtacgct ttctcggcgt ccatcgcgag
     2761 cgcgaggccc accaagcgtc cgcgcgtcgg gctcggctga tccgtaaacg cctcgagcgc
     2821 caggcgtccg ctcgcgcgcg ccgtctcgac gagggtgcgg agctcctcgg cggtgcgtac
     2881 caaatgatgg gttgcgagct gcgcgggggc gggcggaacc tcggccgcgg caggcttcgc
     2941 ggaagggcgt gtgctcggag agggcttcgc gggctcgttc tcgagctgag tcaactgacg
     3001 cgtgaagccg agctcggagt agagctcgcg cagggttgcc acgtcgcgtt ctcggcgccc
     3061 gaagttctcc gggtcgaact ccgcttggca gtcgtccttg agggtgacga ggcgttggct
     3121 cagaaaggcg ttgtcgcggt tgttcgtgag cgtgtcgcgc agccccttac gcggaatctt
     3181 gtcgaggttc tggtaaatac cctcgagcgt tccgaattgg ttcagcagct cttttgcggt
     3241 tttgggcccg actgaaggca ctcccgggat gttgtccgag ctgtcgccgg tgagcgccaa
     3301 gtagtctcgc acttggtcga cccgaacacc gaagcgctcc tcgacctcgg ggacgccgaa
     3361 aactttgtcc cgcatggtgt cgtacatgac gactcgatcg ctcacgagct gcatcaagtc
     3421 cttgtcggcg gccacgatga cgctgcggat gtcgcgcgca cttgcgcact tcacgcagct
     3481 cgcgatgagg tcgtcggctt cgacgccgtc ttgcttcaaa atcggcacct gaaaagcccg
     3541 cacgatttgc tcgaggcgcg cgagctgagt gcgcaggtcc tcgggagcag cgggccgatt
     3601 cgccttgtag gcgtcgtaga tttctttgcg gaaggtggcc cgaccgctgt ccatcgcgat
     3661 cgcgaacagc gctgggcgcc gctcgcgcag cagtcgctcg agcatgctca ccgtgccgtg
     3721 caccgcatgc gtcggctctc cgttggggct cgagagcggc gctaccgcgt gataggcgcg
     3781 cagcacgtag ctcgaaaggt cgaccaggta gagtacgtcg ggagaacccg cggggaatag
     3841 ctcggttgcc atcggggcgc gcgagcctat cgcaggggcc gtcgcttggc ttcctgggtt
     3901 ccaccttaac tgtatgaaaa atcctgcgtc gtcatggatt cggcacattg gcggcgctcg
     3961 ggcgccatcc gggggaggca gttttcctcc cgagaacatg catttggcag cactggatct
     4021 gggatccaat tctttccacc tcttggtcgc ccacgttggg tcgaccggcg cgatcacgaa
     4081 gctcgcgtcg cacaaagagg tgctgaagct cggccgagtg gtccaagaag cgggccgctt
     4141 gcccgagctc gaatacgcga acgcgctcga cgcggtcgcg aagcttacgt cgatcgcgag
     4201 ggcgttccgc gccgagcgac tggtcgttgc ggcgacgagc gcgctgcgcg acgcggcgaa
     4261 tggtcgcgag ttctgccgcg aagtccggga gcgctttggc gttaccgtcg acctgctttc
     4321 tggcgcagaa gaggggcgag tggtgtaccg cggtgcgcga agcgcattcc cggggctgac
     4381 cggtcgcgtc ggcgtcgtcg acattggtgg cggctcggtc gaggtctgcc tgggagaagg
     4441 caatgcctgt cagcatgtcg cgagcctgcc gctcgggttc ctcaaggtcg cagcgaagct
     4501 cgacggtgcg gcgggtcgtg gtgaccggct gcgcgcactg gtgctcgaag agacgcagga
     4561 gctccgcgat cggttgcgtt ggcagcgccc cgaaacgtgg ctgttctccg ggggtacggc
     4621 tcgagccatc ggcaagctgg tcgtcgcggg tccggcgggg atttcgcaag gggccgtacg
     4681 ccgggtcgcc gaggagttgg cgcaggcgtc gcccgagcgt ctccgcgcgc tcggggtgga
     4741 cgcgaagcgc atcgacggga tcgcggccgg ggccttggta ttcgaggcgc tggtcgacgc
     4801 cttctcggtg aagtcactgc gtgtttcacc gggtggtttg cgcgaaggat tgctgttgcg
     4861 ggagctcggg aacatgggcc agccgctcga aacgcgcgac ttcgggctgg aatgggcttc
     4921 tgctggctga tcccgagatg aacgacatct gtcgttaacc gggatagacg ttcacttttg
     4981 gcggaattgg gtcgaaccgt ctcacatcgg gcggctccga ccggactccg ccctttgagg
     5041 gaaacgcggg tccgcgacga gttgttggcg cgcgaagggg agaggggcta gcctcgtcct
     5101 ttggtggctg agaacgatcc gcctcgcacg ttggtcgcag ggaaatacca gctctcgcgt
     5161 ctgctcggac gcgggggaat gggctctgtt tgggagggtg ttcacacgac gctcggaacg
     5221 cgcgtcgcgg tgaagttcat cgaggcggag tacgccaaca gtaccgaagc gcgcagtcgc
     5281 ttcgagaacg aggcgcgcgc cgcggcgaag ctccagagca agtacgtggt cgaggtgtac
     5341 gaccacggcg tcagcgagga cggacggcct ttcatcgtca tggagtacct ccacggcgag
     5401 ccgctggacc gtcgcctcga tcgcgtcggc cgcctcccgg cgaaggagac ggcgttc
//
LOCUS       DASTKS010000897         7472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1931955, whole genome shotgun sequence.
ACCESSION   DASTKS010000897 DASTKS010000000
VERSION     DASTKS010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931955"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFQ35_18695"
     CDS             <1..1134
                     /locus_tag="VFQ35_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932743.1"
                     /translation="ALEFVGAPRTPAFAELPVVLERQSVAGLLQLLLVPALLGAWWLL
                     RGRKRVDDSEPPLRIPLPRPRARLDLLKPQLLIGLLAAVPLTFWLGAVGGRLRAAAAF
                     KALDDQIAPPELVPALVPEAMQGTHGGNRPLFVSAQGIYTRKKTHIVRLEDARRLDGA
                     DCLQVLTGLDLNPDASAGTRREYFPSSVLLDRGADAHTLACSLLYVSLSPQQGDNYAT
                     KVGYGIAAAQFIDPMWQRFLVRNVVPNLPEEYAVFRHSWYEVGLVPTFLGTERYSNRR
                     PPHVHLAKSGWRFRVDSDRDHPAREGTLVGTTDERLARLKEKLDVIYALHVSADTSVP
                     AADVLRIAAAFRSVALYVGADVAAQLEPEREVREPYAGPRPEP"
     gene            complement(1271..1660)
                     /locus_tag="VFQ35_18700"
     CDS             complement(1271..1660)
                     /locus_tag="VFQ35_18700"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9932744.1"
                     /translation="MVHGECGCGRVAFEVEGEFDYAFYCHCSRCRRRTGAACSAIGGV
                     ALDKVRIVAGAEHAVRLDESAKGYRCFCGICFGPLYSAVNGRAHIQLGALTDPPSISP
                     NHHIYVGSKAPWHVITDDLPQHDELPL"
     gene            2134..2763
                     /locus_tag="VFQ35_18705"
     CDS             2134..2763
                     /locus_tag="VFQ35_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9932745.1"
                     /translation="MCTSKLMLAVISSQAPVLRQLPKKQQKVLLIAEWPNSFLPTVRR
                     RQLVNIATYTRSEPIRAVGHVVVPVGDMAYAERFYVGVLGAELRERFDTAPRPVRNSR
                     RSVPLSVRLGGETGTDIFLRRAIPGLERTQSVIGVVVGSALLYPLRARLVSARVPVDT
                     ALSLSFPSAAAIYFVDPFGNKLEVSATAHPRRAEATAPDWAALAYDWRG"
     gene            complement(3232..3522)
                     /locus_tag="VFQ35_18710"
     CDS             complement(3232..3522)
                     /locus_tag="VFQ35_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932746.1"
                     /translation="MNVRTNPSASAALTPFALPDDRGALETTAAGGAGALELGSVEVD
                     SVELGSVELGSVDALARAAVASVPGALAFSRCWQANSSAPAHSAIDTRTHIA"
     gene            3509..4546
                     /locus_tag="VFQ35_18715"
     CDS             3509..4546
                     /locus_tag="VFQ35_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932747.1"
                     /translation="MRTFTEFAAPTEDFISDNLISNETSYLDVAGALAQHRGGVYLGV
                     GPEQNFSYIALARPELALIVDLRRDNALLHLLYKAIFSRARDRTEFLCLLVGRSCARD
                     VAPAADASIEAVLDCVAKAPRDRSQFERDHRVLADDSRVRPELRLSDADFKAMQRMHH
                     QFFERGLDIRFELKSKNGRNYPTLAELLRAHSPDGKYGSFLADESGFGFVRELERQDR
                     VQFAVGDFGKPETLGRIAGELRRRGLVLRTFYTSNVEQYLFGSPAWMAWQANLSAFPI
                     DGESNLLRAYLDQGQRHPLQRHGQRTASFMTPIGAWLECERLKPSKSYFATITRPECG
                     NSSAVRVQADR"
     gene            complement(4707..5480)
                     /locus_tag="VFQ35_18720"
     CDS             complement(4707..5480)
                     /locus_tag="VFQ35_18720"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9932748.1"
                     /translation="MLNFRVAVASTEQEIRDAQRVRFRVYVDEERMLARRAGAEELDS
                     DERDFCPYTTHVLVYRRGEPVGTVRLLRPRTSPDADLGRLDLDLDLSSNFRLSGFARE
                     GLRLGEVTRFCVLRSHRGSSAAALLFSALCRESVRHSITHWVAAANTQTDDAEDAEVL
                     SQIVVKKSLGSPEFAARPLTTAVSREPTRRIYSAEEVRLARAGRCESLELPPVLSLFA
                     RRMGALYIGPPAYDAHFNVFAMPLVSRVRDCTSVAHCSH"
     gene            complement(5474..6208)
                     /locus_tag="VFQ35_18725"
     CDS             complement(5474..6208)
                     /locus_tag="VFQ35_18725"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9932749.1"
                     /translation="MKTAYVRALASQSQRLVQRLDTDPLVGSVVRGTATWQQYVEFLL
                     RTYQYVRWSGLLLAATARGLRASGRARMLVEVVTLKAEEEAPHDRWVLDDLRNLGLPG
                     ERVIREPVPSAIQSYFFWSSTLAEAGSPGFLGAAYALEFVSMQRARQAAENLRARAEI
                     PNVQNALSFLVSHGDADQEHIRVLEETLSSVTDPADVRDIRLSCSVLCTTYPHFFGPH
                     PPLVTELPPRREATPKQERATGGEPC"
     gene            complement(6279..7295)
                     /locus_tag="VFQ35_18730"
     CDS             complement(6279..7295)
                     /locus_tag="VFQ35_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9932750.1"
                     /translation="MTEVYPLLVRLVPADCGALGVSASGRLEDYEWTVADLPTGFFAM
                     YGEMAGHDFVRDSVVRSPNRVLRDQDMIDRTNLERNLFYRRAREVGTPIEQVMAVMLH
                     VDDRWQGGLSLYRDRRAPFSALERDRLQRLAPALANTVRRCQLLERARDLGAALEVLL
                     TKQGGAVVVANARAVELTRSDGATPLLESWFAPSERSEPQLPAPLADALLQFAHEPRQ
                     SSPRRWSRPGASSTLLVDFVELENDRSHGRWMLRFRESRHTTPWPPAWEALLTPRERE
                     VVACIAQGWDNRLIATELGCAETTVKRHLQNIFVKLGVETRSALQVRLSEWRLSSRDP
                     FHDV"
BASE COUNT         1344 a         2346 c         2391 g         1391 t
ORIGIN      
        1 gcgttggagt tcgtgggagc accgcggacg ccggcatttg ccgagctccc agtcgtgctc
       61 gagcgccaga gcgtggcggg cctgctgcag ctcttactcg tgcccgcgct gctcggcgcg
      121 tggtggctgc tgcgcggccg aaagcgggtc gatgactccg agccgccgct gcggatcccg
      181 ctgccgcgcc cccgagcgcg actcgattta ctcaagccgc agctgctaat tgggttgctc
      241 gcggccgtgc cgctcacgtt ctggctcggc gcggtcggcg gtcgcctgcg cgccgccgcc
      301 gcattcaagg ctctcgacga ccagattgcg ccgccagagc tcgtgccagc attggtaccc
      361 gaagccatgc agggcaccca cggcggcaac cggccgttgt tcgtgtccgc ccaaggcatc
      421 tacacccgta agaagactca catcgtgcgg ctcgaagatg ctcgacgact agacggcgcc
      481 gattgcctgc aggtgctcac cggactcgac ttaaatcccg atgcatcggc tggtacgagg
      541 agagagtatt tcccgtcgag cgtgctgctc gatcgaggcg cggacgcgca cactctggcc
      601 tgcagcctgc tctacgtctc gctgtctccg caacaaggag acaactacgc caccaaggtg
      661 ggctacggca tcgcggccgc gcaattcatc gacccgatgt ggcagcgttt tttggtgcgc
      721 aacgtggtcc ccaatctgcc cgaagaatac gcggtcttcc gccactcttg gtacgaagtc
      781 gggttggttc cgacctttct cggcaccgaa cgctacagta accggcgtcc tccgcacgta
      841 catctcgcca agtcaggatg gcgctttcgc gtcgactcgg atcgagacca ccccgcacga
      901 gagggtacac tcgtcgggac caccgacgag cggctcgcac gcctgaaaga gaagctcgac
      961 gtcatctacg ccctccacgt cagtgccgac acctcagtgc ccgccgcaga tgtattgcgc
     1021 atcgcggctg cgttccgaag tgtcgcgctg tacgtcggag ccgatgtggc tgcgcagctc
     1081 gagcccgaaa gagaagtacg cgagccgtac gcagggccgc gacctgagcc gtgatggcgt
     1141 cgagctccgg actggttcgc gctccgcggc gccgtgcagt ggcgcccgcg cctgacgccc
     1201 acatttcgta ctgaacgccc gaagccaatc ctcgtgatgg acggagctcg cgaggtattt
     1261 cgcctgcgcg ctagagcggt aactcgtcgt gttgcggcaa gtcgtcggtg atgacgtgcc
     1321 agggagcttt cgagccgacg tagatgtgat gattggggga gatgctcggc gggtcggtca
     1381 gcgcgccgag ttgaatgtgc gcgcgaccgt tcaccgcgct gtacaatggc ccaaagcaga
     1441 ttccgcaaaa gcagcggtac cccttggcgc tttcatccaa gcgcacggcg tgctcggcac
     1501 ccgcgacgat acgcaccttg tcgagggcaa caccaccgat agcagagcaa gctgcgccgg
     1561 tgcggcgtcg gcagcgcgag caatggcagt aaaaggcata gtcgaactcg ccttcgactt
     1621 cgaacgcaac tcttccgcaa ccgcactcgc cgtggaccat gggcttactc cgcccgagtc
     1681 tcgaacggtt gcggcacaat gtccatccgg agcgtcgatc acgcttccgg catgacgaat
     1741 cgttcgatca acccccgcgt tcgaacttcg ctagcgcggg tccgagtcgt tcgatcgagc
     1801 cccgcgttcg agcttcgcta gcgcgggtcc gaggcgctcg gtgttagccg gaacatccag
     1861 caggggagcg agctctcgaa cgaggagcgc tagttctttc ttggttcgat actccgagcg
     1921 tcgagcgcct cggccagtcc tcaagtgtgg catccacgcc tccgacggga acgcgcaacg
     1981 ggattcggtg cgaagcgtcg ctcaggttaa tcgaatgaac cgcgagcggc gttctggttc
     2041 gctccactcc gccagtagat gccgatagct tcgcggggca agggggtagc agtggaaggc
     2101 taggcgcgac aaggagcggc tggagcgtgc ggggtgtgca catcgaaatt gatgcttgca
     2161 gtcatcagca gccaggcgcc ggttcttcgc caacttccca agaagcagca gaaagtcctt
     2221 cttattgcag agtggcccaa ttctttctta ccaactgtgc gaaggaggca actggtgaac
     2281 attgccactt acacacgtag tgagccgatc cgggctgtgg gccacgtcgt cgtgcctgtc
     2341 ggcgacatgg cttacgccga gcgcttttat gttggcgtgc tcggcgcgga gctccgcgag
     2401 cgcttcgaca ctgcgccgag gccggtgcgc aactcacgcc gcagcgtgcc tttgagcgta
     2461 cggctgggtg gagaaactgg tacggacatc ttcctgcggc gagcgattcc cggtctggaa
     2521 cggacccagt ccgtgattgg tgtcgtcgtc ggcagcgccc tcctgtatcc tctgcgggcc
     2581 cgcctcgttt ccgcccgagt tccagtggac accgcgctgt cgctcagttt cccgagcgcc
     2641 gccgcgatct acttcgtgga tccgtttgga aacaaactcg aggtgtcggc gacggcgcat
     2701 ccgcgacgag ctgaagcgac agcacccgat tgggctgcgc ttgcttatga ttggcgcgga
     2761 tgaaaactgt tcgcgtgacg ttcgaatggc ttcccgccga gcccggcacg gcagatgtcg
     2821 attgcccagg ccgagcagga tctgggcgca gttccggggg cgcatcgaat gcctcccagt
     2881 cgtctccgcc cgtcaacggc tccttcaacg tgcggtcggt tctggttttc gcgtccttgg
     2941 acatcaggta gtcacctggg catggcgatc gttgtcaaag gacgacaact ggagcgggtt
     3001 tcgcatcggg tacaagacct gaaccgggtg ggtgggcgtt gaagaccttc gttcgcaccg
     3061 gaagcgctgt tctttggcaa ttgacagcgc cgttgttgct cgccgtgctc gagtccggcg
     3121 ggaaattcgc gggcgatcga aaggatccat gacggcgcgg gttagggctc gcgaatggat
     3181 aagtcgcttg cgcaggatga cttacccatt cgagggacgt taggttacgt tctaagcaat
     3241 gtgggttcgc gtgtcgattg cgctgtgcgc gggggcgctg ctgttcgcct gccagcaccg
     3301 cgaaaatgcg agcgctccgg gcactgaggc gaccgcggct cgagcgagcg cgtcgacgct
     3361 gccgagttcg acgctgccga gttcgacgct gtcgacttcg acgctgccga gttcgagcgc
     3421 gccggcaccg cctgcggcag tcgtctcgag cgctcctctg tcgtcgggca gtgcgaacgg
     3481 ggtcagggcg gctgaggcgg aagggttcgt gcggacgttc acggagtttg cggcgccgac
     3541 cgaggacttc atcagtgaca atctgatcag caacgagacc tcgtatctgg acgtcgccgg
     3601 cgcgttggcg cagcaccgag gtggtgtgta cctcggcgtt gggcccgagc aaaacttcag
     3661 ctacatcgcg ctcgcgcgcc cggagctcgc tctgatcgtg gatctgcgac gcgacaacgc
     3721 gctgctgcat ctgctctaca aggccatctt cagtcgcgct cgcgatcgaa ccgagtttct
     3781 ctgtctcttg gtgggaagga gttgcgcaag ggatgtcgcg cctgccgccg acgcgtcgat
     3841 cgaagcagtg ctcgactgcg tcgccaaggc gccgcgagac cgttcgcagt tcgagcgcga
     3901 ccatcgagtg cttgccgacg actcccgcgt acggccagag ctccgtttga gtgacgccga
     3961 cttcaaagcc atgcaacgga tgcaccatca gttcttcgag cgaggcctcg acattcgctt
     4021 cgagctcaag agcaagaatg gcagaaacta cccgaccctc gccgagctct tgcgcgcgca
     4081 cagccccgac ggaaagtacg ggagtttttt ggcggacgag agcggtttcg gcttcgttcg
     4141 ggagctcgag cgccaagacc gagtgcagtt tgcggtcggt gatttcggca agccggagac
     4201 gctcggtcgc attgcgggcg agcttcgtcg tcgcggtctc gttctccgta cattctacac
     4261 ttcgaacgtc gagcagtatc tgttcggctc gccagcgtgg atggcctggc aagcgaactt
     4321 gagcgccttt cccatcgacg gcgagagcaa cctgctgcgg gcctacctcg atcagggtca
     4381 gcgccacccg ctgcagcgac acggccaacg cacggcctct ttcatgactc cgattggcgc
     4441 gtggctcgaa tgcgaacggc tcaagccgag caagagctat tttgcgacca tcacgaggcc
     4501 cgagtgcggg aactcgagcg ccgtgcgtgt gcaagccgac aggtgaagcg tgggtgctca
     4561 ccgctgcgag aagcaaaaga acaagttgct ccgtaccgca gccgcgctcg gatcggctgc
     4621 aagtcgggct cgtgaagcgc cagcgccagc ccgcggtgac ccgtcgggtc gcaaagcttg
     4681 gtgtccccga ggctccggca ccgccttcag tgcgagcagt gtgcgaccga ggtgcagtcg
     4741 cgcactctag aaacgagtgg catcgcgaag acattgaagt gcgcatcgta ggcgggggga
     4801 ccgatgtaga gcgcgcccat cctgcgggcg aacaaggaca ggactggcgg aagttccagg
     4861 ctttcacatc ggccagcccg ggcgagcctt acttcttcgg ctgagtagat ccggcgcgtg
     4921 ggctcccgcg agacggcggt cgtcaagggg cgggcagcga actcgggact tcccaagctc
     4981 tttttgacta cgatctgtga gagcacctct gcatcctcgg cgtcgtcggt ttgcgtattg
     5041 gccgccgcga cccagtgagt gatggagtgt cgcacgctct ccctgcacaa cgccgagaac
     5101 aacagcgcgg cggcgcttga gccgcgatgg cttcgcaaga cgcagaagcg cgtgacttcc
     5161 cctaaccgca agccttcccg agcaaagccg ctcagccgaa agtttgagct caagtcgagg
     5221 tcgagatcga gccgtcccaa atccgcatcg ggcgacgttc tcgggcggag caaccgaacc
     5281 gtgccgactg gttcacctcg gcggtacacg aggacgtgcg tcgtgtaagg gcaaaagtcg
     5341 cgctcgtcgc tgtcgagctc ttcggccccc gcgcgccgcg ccaacattcg ctcttcatcg
     5401 acgtagactc gaaaacgcac tcgctgcgca tctcgtattt cctgctccgt gctcgcgacg
     5461 gcgacgcgaa agttcagcat ggttcaccgc ctgtcgcgcg ctcttgtttc ggagtcgctt
     5521 ctcgccttgg cggaagctcc gttacgagag gagggtgggg accaaagaag tgcgggtacg
     5581 tggtgcagag cacgctgcaa ctgagccgaa tgtcacgcac atccgccgga tcggtcacgc
     5641 tactcagagt ctcttcgagc actcgaatgt gctcctggtc cgcgtcgccg tggctcacca
     5701 aaaaggagag cgcgttttgg acattcggga tctcggcgcg cgctcgaagg ttttcggcgg
     5761 cttgacgggc gcgctgcatc gacacgaact cgagcgcgta ggcggcgccg agaaatcccg
     5821 gcgagcccgc ctcggccagc gtggagctcc agaagaagta gctctggatc gcgctcggta
     5881 cgggttcccg aatcacgcgc tcgcccggca atcccaggtt ccgtaagtcg tcgaggaccc
     5941 agcgatcgtg aggagcttct tcttcggcct tcagcgtgac gacctcgacg agcatccgag
     6001 ctcgtccgct agcgcgcagg ccccgcgccg tggcggccag caagagcccc gaccagcgca
     6061 cgtactgata ggtgcggagc aagaactcga cgtattgttg ccaagtcgcg gtaccccgaa
     6121 cgacgcttcc aacgagcggg tccgtgtcga gccgctgcac caagcgctgt gattgcgaag
     6181 cgagcgcacg aacgtaggcg gtcttcatgg gatcctcctg aatgggatcc tgctgaatgg
     6241 gatccttcag aggtacatga agtaagccgg cgccgaagtt acacgtcgtg gaacggatcg
     6301 cgcgagctca ggcgccactc actcaagcgc acctgcagag ccgaacgcgt ctcgacgccg
     6361 agcttgacga atatgttctg caggtgtcgt ttcacggtgg tctccgcaca ccccaactcg
     6421 gttgcaatca ggcggttgtc ccaaccctgg gcgatgcacg ccacgacctc gcgctcccgt
     6481 ggcgtcagga gcgcttccca cgcgggaggc cagggcgtcg tgtgacgcga ctcgcggaag
     6541 cggagcatcc aacgcccatg cgagcggtca ttttcgagtt cgacgaagtc gacgagcaac
     6601 gtcgagctcg cgcctggccg tgaccagcgc cgcggagagc tctggcgcgg ttcgtgcgcg
     6661 aattgcaaga gcgcgtcggc gagtggtgcc ggcaattggg gttcggaacg ttcgctcggt
     6721 gcaaaccacg attcgagcag cggcgtcgcg ccgtcacttc gcgtcagttc gactgctctc
     6781 gcgttggcga cgacaacggc gccgccttgc ttcgtgagca acacctcgag tgccgcaccc
     6841 aggtctcgtg ccctttcgag caattggcac cgacgaaccg tatttgccag agccggagct
     6901 agacgctgca atcgatctcg ctccagcgct gaaaacggtg cgcggcgatc tcggtagagc
     6961 gacaatccgc cttgccagcg atcgtcgacg tgcaacatca ctgccattac ctgctctatc
     7021 ggtgtgccga cctcgcgcgc ccggcggtag aagaggttcc tctccaggtt cgtgcggtcg
     7081 atcatgtcct ggtctcgcag gacgcgattg ggcgagcgca cgacgctgtc tcgaacgaag
     7141 tcgtgccccg ccatttcgcc gtacatggcg aagaaccccg ttggtaagtc ggcgaccgtc
     7201 cattcgtaat cctcgagccg accggaggca gaaacaccga gcgcgccaca atcagctggc
     7261 accaggcgaa ccagcagcgg atacacctcg gtcatcagtt ctcgaacgtc ggccgtaccc
     7321 gcgagcgctt ctaagatctc gagagtcagc tttccgcacg ctgaaccggc ctcggctcca
     7381 tccatattgg tgagccatgg tcgctcaagt cggggtcggt ggaaacacaa agtggagtga
     7441 gcgagaccgg gaatgctact tttggagtat tg
//
LOCUS       DASTKS010000898         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1485741, whole genome shotgun sequence.
ACCESSION   DASTKS010000898 DASTKS010000000
VERSION     DASTKS010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485741"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..959
                     /locus_tag="VFQ35_18735"
     CDS             141..959
                     /locus_tag="VFQ35_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9932751.1"
                     /translation="MSAETFELFMLGGAAEKRYRRMRPEVTALPWGTLDPRAFDPELV
                     LAARKSWTLAAYQEHRTGAACAETVQAMITARVPLDLIAVATRFPLDEMAHVEMCARL
                     AEELGGGTHVEFDPAFLIPSPSSELRPLLRAADCVVRYFCVGEALSIPLLHGTWRAAK
                     HPLVKAVLGRIVRDEAAHGLFGWTFLDWALDELDASDREHLARIAGATIDTLTDSWKT
                     IRGRPESDLDSIHALGWMDSEAYLALAKRSLRAKVLEPLRARGIDPVCNLPPGV"
     gene            1068..1400
                     /locus_tag="VFQ35_18740"
     CDS             1068..1400
                     /locus_tag="VFQ35_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932752.1"
                     /translation="MRSLLIGCLLALGCGSSSTNEPSPNGSCPSLAGAWTVTKHCDAS
                     LVGMTATVNQQTCSLSFASPFNGFSGQVTADGAVTLSGPQSCTGSVANDAIDLTCTPG
                     TCLVTLSR"
     gene            complement(1414..2274)
                     /locus_tag="VFQ35_18745"
     CDS             complement(1414..2274)
                     /locus_tag="VFQ35_18745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9932753.1"
                     /translation="MEPKSIVPEPDNTALRTALWRALHLEADEAPHVFVDDVGIQLAA
                     PDAGWQQRPDMSPFTRPFRASILARARFVEDLVQKRVSEGVGQYVILGAGLDTFAQRR
                     AELGARVVVYEIDQPRAQAWKRERLLALGLGIPSFLRLVPVDFEVGDTWWERLTAAGF
                     DSARPAVVASTGVSMYLTKEAIEATLRQVAALAPGSTLVMSFMLPIEMADPEIRPGIE
                     RAAAGARASGTPFISFFMPEDMLALARNAGFKGVEHVSAASLAERYFAGRSDGLRPPN
                     NAEELVVATT"
BASE COUNT          375 a          843 c          736 g          398 t
ORIGIN      
        1 cccgcggctt cctcgtcgcc tccgcccggc aagcctcccg cggttcagcc agtcggaaac
       61 ttgcgcccgc caacgccgcc gccaactccg cccaaacccc ccaagccgca tccggtcggg
      121 aacctgcgtc ttccggacaa atgagcgccg agacgttcga gctcttcatg ctcggtgggg
      181 ccgccgaaaa gcgctatcga cgcatgcggc ccgaggtcac tgccctgccc tggggcaccc
      241 tcgatcctcg tgccttcgat ccagagctcg tcctcgccgc gcgaaagtcg tggacgctcg
      301 cagcatatca agagcaccgc accggcgccg cttgcgccga aacggtacaa gccatgatca
      361 ccgcgcgcgt cccgctcgac ctgatcgcgg tggcgacccg ctttcccctc gacgaaatgg
      421 cccacgtcga aatgtgcgcg cgcctcgcgg aggagctcgg cggtggcacg cacgtcgagt
      481 tcgacccggc gttcctgatc ccgagcccga gctcggaact tcggccgctg ttacgcgccg
      541 cggactgcgt cgttcgttac ttctgcgtcg gcgaagcgct ctcgattccg ttgcttcacg
      601 ggacgtggcg cgcggccaaa catccgctcg taaaggcggt gctgggccgc atcgtgcgcg
      661 acgaggcggc acacggcctc ttcggctgga cctttctcga ctgggctctc gacgaactcg
      721 acgcgagcga tcgcgagcac ctcgcacgaa ttgcgggggc cacgatagac actctgaccg
      781 actcctggaa aacgattcga ggtcgccccg agtcggacct cgacagcatc cacgcgctcg
      841 gctggatgga ttcggaggcg tacctcgcgc tcgccaaacg ctcgttgcgc gccaaagtgc
      901 tcgagccgct ccgcgcccgg ggaatcgatc cggtctgcaa cctgccgccc ggcgtgtgac
      961 tagaacctac aggagaggcg gggtggtttc gcggcgcggc tttcgcgaac tcgcactctt
     1021 ttacgcaact taacctgctc cggggcccgc cgtacgtcgt cttcgtgatg cgctctctct
     1081 tgatcgggtg ccttctagcg ctgggctgcg gttcgagctc gactaacgag ccttcgccga
     1141 acggctcttg cccgagcctc gccggggcgt ggacggtcac gaagcactgc gacgcttcgc
     1201 tcgtgggaat gaccgccacc gtgaaccaac agacgtgttc actttcgttc gcgtcgccct
     1261 tcaacggctt ctcgggtcag gtcaccgcgg acggagcggt cacgctgagt ggcccacaaa
     1321 gctgtaccgg gagcgtcgcg aacgacgcca tcgacctgac ctgtactccc ggcacttgcc
     1381 tcgtgacgct ctcacgctga cgtcgagacc tgctcaggtc gtcgcgacga ccagctcctc
     1441 cgcgttgttc ggcgggcgca gcccgtcgct ccggcccgcg aagtagcgtt cggcaaggct
     1501 cgccgccgag acgtgctcca cgcccttgaa cccggcgttc cgcgcgagcg ccagcatgtc
     1561 ctccggcatg aagaagctga tgaacggcgt cccgctcgcg cgcgcgcccg cggccgctcg
     1621 ttcgatgccc ggccgaatct ccgggtcggc catctcgatc ggcagcatga acgacatcac
     1681 caaagtcgag ccgggcgcca gcgccgcgac ttgccgtagg gttgcttcga tcgcttcttt
     1741 ggtgaggtac atgctcaccc ccgtcgaagc gaccaccgcg ggccgagcgg agtcgaagcc
     1801 ggcggcggtc aggcgctccc accaggtatc ccccacctcg aagtcgactg gtaccaggcg
     1861 caaaaaagac gggatgccga ggccgagggc aagcaagcgc tcccgcttcc acgcctgagc
     1921 ccggggttga tctatttcgt agacgaccac gcgcgcaccg agctctgcgc gccgctgggc
     1981 gaacgtatct agcccagcgc cgaggatcac gtactgcccc accccctctg aaacgcgctt
     2041 ttgcacgaga tcttcgacga agcgcgcgcg cgcgaggatc gacgcgcgaa atggccgcgt
     2101 gaacgggctc atgtctgggc gctgctgcca gcccgcatcg ggcgccgcaa gctggatccc
     2161 gacgtcgtcc acgaagacgt ggggcgcctc gtccgcctcg aggtgcagcg cgcgccacaa
     2221 tgccgttcgc agcgcggtgt tgtcgggttc gggcactatg ctcttcggtt ccatcggttg
     2281 cctcgcgagg cgcctagact aaccgacgtc gccgctcgtt gcagctacga gcggcggctc
     2341 ctcggaaggg gg
//
LOCUS       DASTKS010000899         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1932539, whole genome shotgun sequence.
ACCESSION   DASTKS010000899 DASTKS010000000
VERSION     DASTKS010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932539"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFQ35_18750"
     CDS             complement(<1..1080)
                     /locus_tag="VFQ35_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9932754.1"
                     /translation="MSELNEPQREAVAHVAGPMIVFAGAGSGKTRTITYRIANLLATE
                     GVPPYRILAVTFTNKAAGEMRERLERLAGPEITRDLWVGTFHSVCARLLRRHYAAVGL
                     TKNYVIYDDSDQKALMTRVLKDMNLSDQSYPPKQMLSLISREKREGNDPEAASLGSRL
                     DSTVASIYKTYQAALLRSDAVDFDDLLHYVTRIAESDGVDGEELRGRFNYVLVDEFQD
                     TNQIQYRLVHALSRRTRNLCVVGDDDQSIYRWRGADVRLIRNFRRDFPDARVVKLEQN
                     YRSSKNIVEAALGVIAPARERVPKVLWTEAQPGDPVRVRAVGDEREEASFVAASIKGE
                     LSRGISPEQVAVFYRVHAQSRALEEA"
     gene            1207..2181
                     /locus_tag="VFQ35_18755"
     CDS             1207..2181
                     /locus_tag="VFQ35_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932755.1"
                     /translation="MTLPSEPFALPATCACCGDSAFSQLGLRSGARRLLIPYCEPCVV
                     HVANDATQTLAERLASLIAGLAACLALPIFLPWWSKVACSAACLVVAALPFARRLLRS
                     KPRPPHTSHGRAAWFSSAALLVCKNERFAQELAARAGTQTEPRAGEKSYRVALAYLGV
                     LAFVLAPLSHAYQHPSVRILNLGDLPFELRVDARALTRVEPSSGESPFAGADVNVPAG
                     RRTFEARDAEGRVVDRTEVEVLAGSHHLYAPASPETCFWLETLGYGRGARKPAYEPLL
                     GTERFWAIPDDVRGWFSPATDANPDARVTGGSARVLRQGPCADVPFGH"
     gene            2229..2759
                     /gene="grpE"
                     /locus_tag="VFQ35_18760"
     CDS             2229..2759
                     /gene="grpE"
                     /locus_tag="VFQ35_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9932756.1"
                     /translation="MSDEPVAIDTQEPEGAAPPASAPADPLEEARAEAARLRDQLLRT
                     AADFDNYKKRARRETQDAERRVREDFLRDLLPVFDNLERAAQHAGTASDVKSLADGIS
                     MVMRLFLDTLGRLGVERVNAVGQAFDPALHEAVQQLETTQFPPGSVAAEVQAGYRMGE
                     RLVRPAMVVVAKPPPS"
     gene            2908..>4057
                     /locus_tag="VFQ35_18765"
     CDS             2908..>4057
                     /locus_tag="VFQ35_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9932757.1"
                     /translation="MGKVIGIDLGTTNSCVSVLEGTTAQGLPDVKVIANSEGARTTPS
                     VVAVTAKGERLVGQVAKRQATTNPENTIYAVKRLMGQRFDAKAVEHQQRTSPFRIVKH
                     QNGDAWVAVQGKELSPPEVSSFVLTTMKEVAEAYLGEPVTEAVITVPAYFDDAQRQAT
                     KDAGRIAGLEVKRILNEPTAAALAYGLDKKEPQVIAVYDLGGGTFDISILEMQKGVFS
                     VRATGGDTHLGGEDFDALIIEKLAAEFLAEHKVDLRADRMSLQRLREAAEKAKHELSP
                     SLETEINVPFITSGPSGPLHLQRTMKRSELEILCAPLIERTMVACKAVLADAKLTVDK
                     IGAVVLVGGMTRMPAVQRAVRELFGRDPHKGVNPDEVVSVGAAIQAGAI"
BASE COUNT          662 a         1356 c         1347 g          692 t
ORIGIN      
        1 cgcttcttcc agcgcgcgcg actgagcgtg cactcggtag aacaccgcga cctgctcggg
       61 ggagatcccg cgggagagct cgcccttgat cgacgcggct acgaagctcg cctcttcgcg
      121 ctcgtcaccc acggcccgca cgcgcaccgg atcgcctggt tgcgcttcgg tccacagcac
      181 cttcggcacg cgttcgcggg ccggggcgat gacgcccagc gccgcctcga cgatgttctt
      241 gctcgagcgg tagttttgct cgagcttgac cacgcgcgcg tccgggaaat cgcgacgaaa
      301 attgcgaatg aggcggacgt cggcgccgcg ccagcgataa atcgactgat cgtcgtcgcc
      361 caccacgcac aggtttcgcg tgcggcggct cagcgcgtgc acgaggcggt actggatttg
      421 gttggtgtct tggaactcgt cgaccaagac gtagttgaag cggccgcgga gctcctcgcc
      481 gtcgacgccg tcgctctccg cgatgcgcgt tacgtagtgc aagaggtcgt cgaaatcgac
      541 ggcgtccgag cgcaagagcg cggcttgata ggtcttgtag atgctcgcga cggtggagtc
      601 gagccggctg ccgagcgacg ccgcttcggg gtcgttccct tcgcgcttct cgcggctgat
      661 gagcgagagc atttgcttcg gcgggtacga ctggtcgctc aagttcatgt ccttgagcac
      721 gcgcgtcatg agcgcctttt ggtccgagtc gtcatagatg acgtaattct tggtcaagcc
      781 gacggcggcg tagtggcggc ggagcaggcg cgcgcacacc gagtgaaagg tgccaaccca
      841 gagatcccgc gtgatttccg gacctgcgag gcgctcgagg cgctcgcgca tctctcccgc
      901 ggccttgttg gtgaaggtca cggccaggat tcggtacgga ggtacgcctt cggtagcgag
      961 caggttcgcg atgcgatagg tgatggttcg cgtcttgccg ctgccggcgc ccgcgaacac
     1021 gatcatcggg ccggccacgt gggcgacggc ttcgcgttgc ggctcgttga gctcggacat
     1081 cctcctcggc gtatgacatc ggacgactct cggcaacgtg tggctcgcag cgcccccgac
     1141 ctttcctgcg agacggccga aacgcgagaa tttcgcgcgc cgccgctgga ccacagtgcc
     1201 ttcggcgtga cgcttccgag cgaacccttc gcgctgcccg cgacgtgcgc gtgctgtggg
     1261 gactcggcct tctcgcagct cggccttcgg agcggcgcgc ggcggctcct gattccgtac
     1321 tgtgagccgt gcgtggtcca cgtcgccaac gacgcgacgc agacccttgc cgagcgcctt
     1381 gcgtcgctca tcgcgggcct cgcggcgtgc ctcgctctcc ccattttcct gccctggtgg
     1441 tcgaaggtcg cttgttccgc ggcgtgcttg gtcgttgcgg cactgccgtt cgcgcgccga
     1501 ctgcttcgct cgaagcctcg cccgccccac acctcgcacg ggcgcgcggc ttggttttcg
     1561 agcgccgcgc tcctcgtttg caagaacgag cgctttgcgc aagagctcgc ggcgcgcgcc
     1621 ggaacgcaga cggagccgcg cgccggcgag aaatcctacc gagttgcgct cgcgtacctc
     1681 ggagtgctcg ctttcgtgct cgcgccgctg tctcacgctt accagcaccc gagcgtccgc
     1741 atattgaacc tcggggactt gcccttcgag ctccgcgtcg acgcacgcgc cctcactcgc
     1801 gtcgagccgt cgagcggaga gagcccgttt gccggcgccg acgtgaacgt ccccgcgggg
     1861 cggcgcacgt tcgaagcgcg cgacgccgaa ggcagggtcg tcgaccgcac cgaagtggaa
     1921 gtgcttgcag ggtcgcacca cctgtacgct cccgcctcac cggagacctg cttttggctc
     1981 gaaaccctcg gctatggcag gggagcacgc aagccagcct acgagcccct gctcggaacc
     2041 gagcgctttt gggcgattcc cgacgacgta cgcggttggt tctccccggc gaccgacgcg
     2101 aacccggacg cgcgcgtcac cggaggcagc gcgcgggtgc tgaggcaggg tccctgcgcg
     2161 gacgtgcctt tcgggcactg acgcctgtgc ccgttggcaa tgtcgaacga gtggttatac
     2221 tggcgcgcgt gtcggacgaa cccgtagcca tcgataccca ggaacccgaa ggtgccgccc
     2281 cgcccgcgtc agcgccggcc gaccccctcg aagaggcccg cgccgaggct gcgcgtctgc
     2341 gcgatcagtt gctgcgcacg gcggctgact tcgacaacta caaaaagcgc gcgcgccgcg
     2401 agacccaaga cgcagagcgc cgggtccgcg aggatttttt gcgtgatcta ctgcccgtct
     2461 tcgacaacct cgagcgggcc gctcaacacg ctggaacggc gagcgacgtg aagtcgttgg
     2521 cagacggcat cagcatggtg atgcgtctct tcctcgacac cctcggtcgc ctcggtgtcg
     2581 agcgcgtcaa cgcagtgggt caagccttcg accccgcgct ccacgaggcc gtgcagcagc
     2641 tcgaaacgac gcagtttccg cctggctcgg tcgcggccga agtgcaggct ggttaccgca
     2701 tgggcgagcg tctcgtgcgt ccggcgatgg tcgtggtcgc aaaaccgcca ccctcttgag
     2761 tgggtgagta gcagcgctcg cagtatgtga gtgagcgctg atggggcctc ggtcgtccgc
     2821 cgtgcgagaa ggtacgtgcg acgatcggcc cttcgttcag cacgatctgc caggtttcgc
     2881 tccgcgcgaa ggagtatcct cgtctcgatg gggaaagtaa ttggcatcga cctgggcacc
     2941 accaactcct gcgtatcggt gttggaaggc acgaccgccc agggccttcc cgacgtgaag
     3001 gtgatcgcca acagcgaagg cgcgcgcacc actccctccg tcgtcgccgt caccgccaag
     3061 ggcgagcgct tggtcgggca ggtcgccaaa cgacaagcaa cgacgaaccc cgaaaacacg
     3121 atctacgcgg tcaagcgtct catggggcag cgcttcgacg cgaaagcggt ggagcatcag
     3181 cagcgcacct cgccgttccg catcgtgaag caccaaaacg gcgacgcgtg ggtcgcagtg
     3241 cagggcaagg agctctcgcc gcccgaggtg tcgagcttcg tgctcaccac catgaaggag
     3301 gtcgcggagg cgtatctggg cgaaccggtg actgaagcgg tcatcaccgt ccctgcgtac
     3361 ttcgacgacg cgcagcgcca agccaccaaa gacgccggcc gcatcgcggg gctcgaggtc
     3421 aaacgcatct tgaacgagcc gaccgcagcg gccctcgcgt atggtctcga caagaaagag
     3481 cctcaggtca tcgccgttta cgacctcggc ggcggcacct tcgacatctc catcctggag
     3541 atgcaaaagg gcgtcttcag cgtgcgcgcg accggcggtg acacccacct cggcggcgag
     3601 gacttcgacg cgctgatcat cgaaaagctc gcggccgagt tcctcgccga gcacaaggtc
     3661 gacctgcgcg ccgaccgcat gtcgttgcag cgcttacgtg aagcagccga gaaagcgaag
     3721 cacgagctct cgccgtcgct cgagaccgag atcaacgtgc ccttcatcac gtcggggccg
     3781 agcggcccgc tccacttgca gcgcacgatg aagcgcagcg agctcgagat cctgtgcgcg
     3841 ccgctcatcg agcggacgat ggttgcttgc aaggccgtgc tggccgacgc caagctcacc
     3901 gtcgacaaga ttggcgccgt ggttttggtg ggggggatga cgcgcatgcc ggcggtgcag
     3961 cgcgccgtgc gcgagctgtt cggcagggac ccgcacaagg gcgtcaatcc ggacgaagtc
     4021 gtttcggtcg gcgcagcgat ccaggcgggc gcgatca
//
LOCUS       DASTKS010000900         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1485828, whole genome shotgun sequence.
ACCESSION   DASTKS010000900 DASTKS010000000
VERSION     DASTKS010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485828"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFQ35_18770"
     CDS             complement(<1..425)
                     /locus_tag="VFQ35_18770"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9932758.1"
                     /translation="MWKFLIRTILQRGVLLFFISIISHAVIHLAPGAPTEVDPMNPRM
                     KAADVVKLRKAFHLDEPLHEQYVHWARDLFSGELRSFKDGAPVLPRIWERFLNSLPLF
                     VCSTLITWTLSFPLGIRAAVKRGAAFDRSTTVLAYALIS"
     gene            complement(428..2566)
                     /locus_tag="VFQ35_18775"
     CDS             complement(428..2566)
                     /locus_tag="VFQ35_18775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9932759.1"
                     /translation="MFTRRLLLLLPIVLAVLLFQSALWVPSYGSQTHGHPERLTTFVQ
                     ASIGDAKLLNPVLAADTASSTLVSRRLFEALLDVNERLEVVGRLAERFELTELAYLAA
                     IPERTLPDGRAATGSALLETLRSALDAGKLPDVAPLVKGLRVVAPGERALNEEVVHTS
                     AKGKPEPYEIKGRIELPERVEIQLSRVTPKFFDALRPLLGPEYFEKYPFANRFKLENP
                     KDLAELRDKLPQLLPIGEHNPKLTFYLRHGVRFHDGKPFSAEDVKASFEAYTDGKTAS
                     PRAASFEPVRELTVVDPYTVEVLYKRLYAGALVDWMLEIVPSNHVNKAALEREMNERK
                     MSASERAAFSLRQSRFNQNPVGTGPFRFDKWERDDYVHLTRNDDYWGKKPEYRDFYFR
                     AIPDYVSQELELHAGAIDMYEPLPHQVARYKHDDRYQVVSGSEGYYVYIGYNLRKPLF
                     QDVRVRRALGMAINVDELIKYVLYGEGKRTSGPYYSYTPFYDPETPLLPYDPKAAADL
                     LASAGWVRNKAGWLEKNGQELAFTLITNNGNPQRKAIMTIAQDAWQKLGVRCTSQAFE
                     WTAFLEDFIETNKFDAYVLGWVGGDINPDKFDIWHSSQTNAYQLNHVGYKDAEADRMI
                     EELRQEYDRPTQVRLARALHRKIANDQPYTFLYEPTKPYVLDKRIFVVGKGPDGKETY
                     SRIQPTPSGDVSYYFDYWRKLPASAHAAEN"
     gene            complement(2622..3734)
                     /locus_tag="VFQ35_18780"
     CDS             complement(2622..3734)
                     /locus_tag="VFQ35_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932760.1"
                     /translation="MDTRSSPPKIATSEFPPEPTGLKGRRSELRTLSEMVRATHPTRI
                     ALVGSGGSGKSMLAAALGHRLKRAFGGRADWFRVGAWDFRTVTEMFALRFGMPRDERR
                     ERRLQRFFSQSAPRLIVLDNHENDRAMARLLETFANGNVSFIITARRCLLAGVLIFPV
                     TAPLVTAGESAFPRVAGLTRLLRWNPLALDIADAIVRSRGTSATELAEFLRERGVARV
                     RAQVHEDDLPEVALLLDWAWRRLSREARRMLGVLAHLEGDHIDVESLRTLARLRSSPN
                     PGIEELRRWHLIQEPARSRYTLHAVVRHALAQRTKPAPERTFEHYVSMLERQPDRFAA
                     EQTHLFAAMDYAYRESDMKAILRVDALMRRLDGDTT"
BASE COUNT          708 a         1208 c         1323 g          739 t
ORIGIN      
        1 atggaaatca aagcgtaggc gagcaccgta gtgctgcgat cgaacgcagc gccgcgcttc
       61 actgcggcgc ggattccgag cggaaacgag agcgtccagg tgatgagcgt gctgcaaacg
      121 aagagcggca gcgaattcaa gaagcgctcc caaatgcgcg gaagcaccgg agcgccgtcc
      181 ttgaacgagc ggagctcccc gctgaacagg tcgcgcgccc agtgcacgta ctgctcgtgc
      241 agcggctcgt ccaggtggaa ggctttgcgc agcttcacga cgtcggcggc tttcattcgt
      301 ggattcatcg ggtcgacctc ggtgggcgcg ccgggggcga ggtgaatcac tgcgtgcgag
      361 atgatcgaga tgaagaacaa gagcacgccg cgctgcagga tcgtgcgtat caagaacttc
      421 cacatgctca gttctccgcg gcgtgggcgc tcgccgggag cttgcgccag tagtcgaagt
      481 agtagctgac gtcgccagac ggggtcggct gaatgcgact gtacgtctct ttgccgtccg
      541 gacccttgcc gacgacgaag atgcgcttgt cgagcacgta gggcttggtc ggctcgtaaa
      601 ggaacgtgta cggctggtcg ttcgcgattt tgcgatgcag cgcgcgcgcc agacgcacct
      661 gcgtcgggcg gtcgtactct tgccgtagct cctcgatcat gcggtcggct tcggcatctt
      721 tgtagccgac gtgattcagc tggtaagcgt tggtctggct cgagtgccag atgtcgaact
      781 tgtcggggtt gatgtcgccg cccacccagc cgagcacgta cgcatcgaac ttgttcgtct
      841 cgatgaagtc ttcgaggaag gccgtccact cgaaggcttg gctcgtgcag cgcaccccga
      901 gcttctgcca ggcgtcctgc gcgatcgtca tgatcgcttt gcgctgcgga ttgccgttgt
      961 tggtgatcag cgtgaacgcg agctcttggc cgttcttttc gagccagccc gccttgttcc
     1021 tcacccagcc cgcgctcgcg agcaggtccg ccgccgcctt gggatcgtaa ggcaagagag
     1081 gcgtctcggg atcgtagaac ggagtgtacg agtagtaagg accgctcgtg cgcttgcctt
     1141 ctccatacag cacgtacttg atgagctcgt cgacgttgat ggccatgccg agcgcgcgcc
     1201 gcactcgcac gtcttgaaag agcggctttc gcaagttgta gccgatgtaa acgtagtagc
     1261 cctccgagcc cgagacgact tgatagcggt cgtcgtgctt gtagcgcgcg acctggtgcg
     1321 gcagcggctc gtacatgtcg atcgcccccg cgtggagctc gagctcctgc gaaacgtagt
     1381 cggggatcgc gcggaagtag aagtcgcggt actcgggctt tttgccccaa tagtcgtcgt
     1441 tacgagtcag gtggacgtag tcgtctcgct cccacttgtc gaagcgaaac ggccccgttc
     1501 cgactgggtt ttggttgaag cgagattggc gcaacgaaaa cgcagcgcgt tcactcgccg
     1561 acatcttgcg ctcgttcatc tcgcgctcga gcgcggcctt gttcacgtgg ttcgacggaa
     1621 cgatttcgag catccagtcg acgagcgcgc cggcgtagag acgcttgtag agcacctcca
     1681 ccgtatacgg gtcgacgacc gtgagctcgc gcaccggttc gaagctcgcc gcgcgcggcg
     1741 acgccgtttt cccgtcggtg taagcctcga agcttgcctt gacgtcttcg gcgctgaacg
     1801 gcttgccgtc gtggaagcgc acaccgtggc gaaggtaaaa ggtgagcttc gggttgtgtt
     1861 cgccgattgg caagagctga ggaagcttgt cgcggagctc cgccaagtct ttggggttct
     1921 cgagcttgaa gcgattcgcg aacggatact tttcgaagta ttcggggccg agcagcgggc
     1981 ggagcgcatc gaagaatttc ggcgtcacgc gcgagagctg gatctcgacg cgttccggca
     2041 gctcgattcg gcccttgatt tcgtaaggct ccggcttccc cttcgcggag gtgtgcacga
     2101 cctcttcgtt cagcgcgcgc tcgcccggcg cgacgacgcg aagccccttc acgagtggcg
     2161 cgacgtcggg cagctttccc gcgtcgagcg ccgagcgcag cgtctcgagc agcgcgctcc
     2221 cagtcgcggc acgcccgtcc ggcagcgtgc gctccgggat cgctgctaaa taagcgagct
     2281 cggtgagctc gaagcgctcc gcgagacgcc cgactacttc gaggcgctcg ttcacgtcga
     2341 ggagcgcttc gaacagacgc cgtgatacca gcgtggagct cgcggtgtcg gccgccaaca
     2401 ccgggttcaa gagcttggcg tcgccgatgc tggcctggac gaaggtcgtg aggcgctcgg
     2461 ggtgcccgtg cgtttgactg ccgtagctcg gcacccacaa cgccgactgg aagaggagca
     2521 cggcgagcac gatcggaagc aataagagga gccgacgtgt gaacatgccg aacgttgcga
     2581 ggcctcagca aaccccctgg cgctctccgc gtcaacccgc ttcaggtagt gtcgccgtcg
     2641 agccggcgca tcagcgcgtc gacgcgcaaa attgccttca tatcgctctc gcgataggcg
     2701 tagtccatcg cggcgaagag gtgcgtctgc tcggcagcga agcggtcggg ctgccgttcg
     2761 agcatcgaca cataatgctc gaaggtgcgc tctggcgccg gtttggtgcg ctgggcgagc
     2821 gcgtggcgga cgacagcgtg cagcgtgtag cgcgaacgcg caggctcttg aatcaggtgc
     2881 cagcggcgga gctcctcgat gccggggttc ggcgagctac gcagccgagc gagcgtccgc
     2941 agagactcga cgtcgatgtg atcgccctcc aggtgcgcga gcacgccgag catgcgccgc
     3001 gcttcgcgcg agagacgcct ccaagcccaa tcgaggagta gcgcgacctc cggtaagtcg
     3061 tcttcgtgca cctgcgcgcg gactcgggcg accccgcgtt cgcgaagaaa ttcggcgagc
     3121 tccgtcgcgc tcgtccctcg cgaacggacg atggcgtcgg cgatgtcgag cgcgagcggg
     3181 ttccaacgca agagccgcgt caacccggca acgcggggga aggcgctctc gccagcggtc
     3241 acgagcggcg cggtcacggg gaagatcaag acgcccgcga ggaggcagcg ccgcgcggtg
     3301 atgatgaacg agacgttgcc gttcgcgaag gtctcgagca agcgagccat ggcgcgatcg
     3361 ttctcgtgat tgtcgagcac gatgaggcgc ggcgcgctct gggagaagaa cctttgcagc
     3421 cgacgttcgc gccgctcgtc acgaggcata ccaaagcgca gcgcaaacat ctcagtcacg
     3481 gttcgaaagt cccaggcgcc cacgcgaaac caatcggctc ggcccccaaa ggctcgcttc
     3541 agccggtgac caagcgcggc ggcgagcatg gattttccgc tgccgccgct accgacgagg
     3601 gcgatgcgcg tgggatgcgt ggcacgcacc atttccgaaa gggttcgcag ctcgctgcgc
     3661 cggcccttca ggccagttgg ttccggtgga aactcggagg tcgcgatctt cggtggggag
     3721 ctacgtgtat ccatttgcga cattcgttcg cgctgtgcgt cactctggag agtgttggca
     3781 aaagatggct ttccggccgt ccgaaaagtg tcggtttccg tcaagtttcg cgcgcgcaac
     3841 gctctgcgga gcgcttgtgg tcacatgcat gaaacgtggc tgcaaggtcg gccgagtgat
     3901 actgaaccga cttgattaga gcctccctac tcgagacccc aattcgcacc ggcgcaaaca
     3961 acgaatcgga aacgtccg
//
LOCUS       DASTKS010000901         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_145875, whole genome shotgun sequence.
ACCESSION   DASTKS010000901 DASTKS010000000
VERSION     DASTKS010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145875"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1171)
                     /locus_tag="VFQ35_18785"
     CDS             complement(53..1171)
                     /locus_tag="VFQ35_18785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9932761.1"
                     /translation="MTSELGIGVLGLGSAGARHARTIAAGAVPGAFLAAVCDPIAERR
                     SGFDAFGASDAEELLKRPDVGAVVVATPHRTHVELGIAAIRAGRHVLMEKPLGVHKAE
                     CERVVAAHRAAHAAGLTFGVVHDYRAHPRFQWLRAQLNRGDLGRVERVVWQATDWYRT
                     HAYYRGSSWRGRYASEGGGLLVNQLPHLFDTLIWLFGAPRKVLGQCRFGRFHEVEVED
                     DVTALIDFAGGHSAVVVASTGESPGTNRLEVSCDRGRIVLEDGAALVHRSREASSSHR
                     RRESSGRPLADVERLEFPARAATGLALLTNFVAAIAGREPLLAPAEQAIAAVETANAV
                     LWSSLLGRALTLPIDAEAFEGVLAELSASGAATALGSG"
     gene            1362..2159
                     /locus_tag="VFQ35_18790"
     CDS             1362..2159
                     /locus_tag="VFQ35_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9932762.1"
                     /translation="MTARAPAPPVGKVDRAALGAAEQARLVGTLALMLTAIFRSFGKG
                     DFDRGELVLNLHRMGVKSLPVVVTTALFTGGIMVIQAGPIVERYGAQGLLGWGAGFGT
                     LREIGPLLTALMISGRVGANNTAELGTMAVTEQIDALRALAIDPIGFLVGPRFIAIVV
                     TLLLSTLFADALALFGAAQCGWALLGVEPSVFYRGLTGELLGFGDVLHGLLKSVGFGI
                     VIGLSSCHFGITTRGGAPGVGRSVNATVVVSAAGIFGLDYLLSFVLQ"
     gene            2156..2980
                     /locus_tag="VFQ35_18795"
     CDS             2156..2980
                     /locus_tag="VFQ35_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9932763.1"
                     /translation="MTTGLPSREPLARARPAPRVEQPRALPGLLHAAHELYTTFAQTL
                     YSVARGPRDRSAVVYQMYEIGNRSLIFVSVVMGFIGMILVFQGGLQTQRVVPDLSGLG
                     AQFLELLVRDLSASICALMLATRVGAGIAAEIGSMVVTEQIDALRMCAADPIDYLVRP
                     RFLASLFMTVVLVVIAGSVAFASGMGAAYTFFGINPRTFTNLSLVDAGDVTVGLAKCF
                     AYGAAIPVVSAHAGLTTFGGSAGVGAATTRAVVNSSLAVIALDFIISGLGYIVFGP"
     gene            complement(3062..3688)
                     /locus_tag="VFQ35_18800"
     CDS             complement(3062..3688)
                     /locus_tag="VFQ35_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932764.1"
                     /translation="MSTKAKLRLVLGLVALGVGCASSEPQPKAKRDGAASLRCDVDEV
                     RESYCEGLVPLSTAVGAPPPYQNCPAGIEVPSAAAAYPPSERVARFDARNTEATRRRV
                     QPGHSCCYSWCAKVAVADPNDVLPEAGCSQPGAARETYCFPELEGGSKDPGPAPYGRC
                     PLAIRPPENVSFSVPKAAPLDLATTQFKRAGGEASCCYGWCSRMPVGI"
     gene            3819..4562
                     /locus_tag="VFQ35_18805"
     CDS             3819..4562
                     /locus_tag="VFQ35_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9932765.1"
                     /translation="MISFRQITKTFGTKRVLDGVSFEVEPGEVFFIIGASGVGKSVLI
                     KQLIGLLQPDSGEIWLDNENLTHLDEAGFYAVRKKCALVFQHSTLFDSMTCAENVALP
                     LRKHHGYGKAEALDHARRELDRVQMLPFAERFPNELGDGMRKRVAIARALTMQPRYVL
                     FDEPTTGLDPVSSRRVDHLIRTLCDERGVTSVVVSHDLTSIFTIADRIAMLYKGKVRL
                     LGTPQTFQASDDGVVRQFIRGEADGPMEF"
BASE COUNT          726 a         1556 c         1516 g          809 t
ORIGIN      
        1 ggcggggact tgggacaccc cctagcgcgt ttcgggaggg gggttgctcc gttcaacccg
       61 agccgagcgc cgtcgccgcg ccgcttgcgg agagctcggc gagcacccct tcgaaagctt
      121 ccgcatcgat cggtaacgtc aacgcgcggc cgagcaggct cgaccagagc acggcatttg
      181 cggtctccac ggccgcgatg gcttgctcgg cgggagcgag cagcggttcg cggccggcga
      241 tcgccgcgac gaagttcgtg agcaaggcaa ggcccgtcgc cgctcgcgcc gggaactcga
      301 gccgttcgac gtcggcgagt gggcgcccgg agctctcgcg gcgccggtgg gagctcgaag
      361 cttcgcggct tcgatgcacg agcgcggcgc cgtcctcgag cacgatgcgc ccccggtcgc
      421 acgacacctc gagccgattg gtccccgggg actcgccggt gctcgcgacg acgacggcgc
      481 tgtgccctcc cgcgaaatcg atgagcgcgg tcacgtcgtc ttccacctcc acctcgtgaa
      541 agcggccgaa acggcactgc ccgaggacct tccgcggcgc cccgaacagc caaatcagcg
      601 tgtcgaaaag gtgcggtagc tggttcacga gcagcccgcc gccttcgctc gcgtagcggc
      661 cgcgccagga gctgcctcga tagtaggcat gggtccggta ccagtcggtc gcttgccaga
      721 ccacccgctc gacgcgcccg aggtcacccc ggttgagctg cgcgcgaagc cactgaaagc
      781 gcgggtgcgc gcggtagtcg tggaccacgc cgaacgtgag ccccgcggcg tgagctgccc
      841 gatgcgcggc aacgacgcgc tcgcactcgg ccttgtgcac gccgagcggc ttttccatca
      901 gaacgtgccg ccccgcgcga atggcggcga tcccgagctc gacatgcgtg cgatgcggtg
      961 tcgcaacgac caccgcgccc acgtcggggc gcttcaacag ctcctcggcg tcgctcgccc
     1021 caaaggcgtc gaaccccgag cgacgttccg cgatggggtc gcacacagcc gccaaaaagg
     1081 cgcccgggac ggcgcccgcg gcgattgttc tcgcgtggcg cgcacccgcg ctaccgaggc
     1141 cgagaacacc gatgccaagc tcgctcgtca aaagagagcc ggaaactagt cacaggcacc
     1201 cttccgcgcc agcgaaaatg gaaagcaggc gcgcctcgac ggtgcggccc caaatctgcg
     1261 agggacttgg cccgccggga gccgagggct gccggatccg aaccgtacgg ccatcaactt
     1321 tcgcgaaacg ggactgtcgc gcagtgtaaa ctcgcggctc catgaccgcg cgcgcccccg
     1381 ccccgcccgt cggcaaggtc gaccgcgctg cgctcggcgc cgccgagcaa gcccgattgg
     1441 tggggacgct cgcgctcatg ctcaccgcga tattccgctc gttcggtaag ggcgacttcg
     1501 accggggcga gctcgtcctg aatctccacc gcatgggcgt gaagagcctt cccgtcgtcg
     1561 ttacgaccgc gctcttcacg ggtgggatca tggtgatcca agcaggacct atcgtggagc
     1621 gctacggagc ccaaggcttg ctgggctggg gcgccgggtt tggaacgctg cgtgaaattg
     1681 gaccgctcct caccgcgctc atgatttcgg gtcgggtcgg tgcgaacaac accgcggagc
     1741 tcggcacgat ggcggtcacg gagcagatcg acgctttgcg cgcgcttgcg attgacccca
     1801 tcggcttcct cgtcgggccg cgcttcatcg cgatcgtggt cacgctgctg ctctcgacgt
     1861 tgtttgccga tgcgctcgcg ctcttcgggg cggcgcaatg tggctgggcg ctgctcggcg
     1921 tggagccctc ggtattttat cgcggcctca ccggcgagct cctcgggttc ggcgacgtgc
     1981 tgcacggctt actgaaaagc gtggggttcg gcatcgtgat cggcctatcg agctgtcact
     2041 tcggaatcac gacgcgcggc ggcgctcccg gcgtcgggcg cagcgtgaac gcgaccgtgg
     2101 tcgtgagcgc tgccggcatc ttcgggctcg attatctgct ctcgttcgtg ctgcaatgac
     2161 gaccggcctc ccttcgcgcg aaccgctcgc ccgcgctcga cctgctccgc gcgtcgagca
     2221 accgcgcgcg ctgcccggtc ttctgcacgc ggcccacgag ctctacacga ccttcgcaca
     2281 gactctttac agcgtcgccc gcgggccacg agaccgttcg gcggtcgtct atcaaatgta
     2341 cgagatcggc aatcgttcgc tgatcttcgt gtcggtggtg atgggcttca tcgggatgat
     2401 cttggtcttc caagggggcc ttcagaccca gcgtgtcgtg ccggatctct ccgggctcgg
     2461 cgcgcaattc ttggagctct tggtgcggga tctgtcggct tcgatttgtg ccctgatgct
     2521 cgcgacgcgc gtgggcgctg gcatcgcagc cgaaatcggc tcgatggtgg tcaccgagca
     2581 aatcgacgcc ctgcgcatgt gcgccgccga ccctatcgat tatttggtaa ggcctcgttt
     2641 tttggcttca ttgttcatga cggtggtgct cgtcgtgatc gcgggcagcg tcgctttcgc
     2701 gagcggcatg ggcgccgcct acacgttctt cggaatcaac ccgcgcacct tcacgaacct
     2761 gtcgctcgtc gacgccggcg acgtcacggt cggcctcgcc aagtgcttcg cgtacggcgc
     2821 tgccatcccg gtcgtcagcg cccacgccgg cttgacgacg ttcggcggct cggcaggcgt
     2881 cggtgccgcg acgacgcgcg ccgtcgtgaa ctcgtcgctg gccgtgatcg cgctcgactt
     2941 catcatcagc ggcctcggct acatcgtgtt cgggccgtag gcggtcccaa ggcgcggccc
     3001 gctttcgaga tggaccggcg tctcaaattc cgactgcggc ctttcgagat ggaccggcgt
     3061 ctcaaattcc gactggcatg cgcgagcacc acccatagca gcagctcgcc tcgcctcccg
     3121 cgcgcttgaa ctgagtggtt gcgagatcga gcggggcagc cttggggacc gagaacgaga
     3181 cgttttcggg cgggcgaatc gcgagcggac agcggccgta cggcgccggc ccggggtctt
     3241 tgctgccgcc ttcgagctcc ggaaagcagt atgtttcacg cgcggcgccc ggctggctgc
     3301 agccagcttc gggtaaaacg tcgttcggat ccgcgacggc gaccttggcg caccagctgt
     3361 agcagcagga gtgaccgggc tgaacgcgtc ggcgcgtcgc ctcggtgttg cgagcgtcga
     3421 agcgagccac gcgttcgctc ggtggataag cggccgcggc gctcggcact tcgatcccgg
     3481 cggggcaatt ctggtacggg ggcggtgccc caaccgcggt cgagagcggc accaggccct
     3541 cgcagtacga ctcgcgcacc tcgtcgacgt cacatcgcaa agacgcggcg ccgtcgcgct
     3601 tcgctttcgg ctgtggctcc gagctggcgc agccgacccc gagcgcaacg agcccgagca
     3661 cgagacgcaa ctttgctttc gtcgacaccc gacctgtccc gcaaactttg gctttcgcca
     3721 acaccgttcg gtatactgga tggggtcgca ttgtcaaagc acagcgcccg cgtcccaggt
     3781 cgttttcgag gcccgctccg taccttttcg accgagcgat gatctcgttc cgacagatca
     3841 cgaagacctt cgggacgaag cgcgtgctcg acggcgtaag cttcgaggta gagccgggcg
     3901 aagtcttttt catcatcggg gcatccggcg ttggcaagag cgtgctcatc aagcagctga
     3961 tcgggctcct ccagccagac tccggggaaa tttggctcga caacgagaac ctcactcacc
     4021 tcgacgaggc cggcttctat gccgttcgaa agaaatgcgc cctcgttttc cagcactcga
     4081 ccctgttcga ctcgatgact tgcgccgaga acgtggcgct gcccctgcga aagcaccacg
     4141 gatacgggaa ggccgaggcc ctcgaccacg cgcggcgtga gctcgaccga gtgcagatgc
     4201 tcccatttgc ggagcgattt cccaacgagc tcggcgatgg gatgcgcaag cgggtcgcca
     4261 tcgcgcgcgc cctcaccatg caacctcgct acgtcttgtt cgacgagcca acgactggcc
     4321 tcgaccccgt gagctcccgc cgcgtagacc acctgatacg tacgttgtgc gatgagcgcg
     4381 gcgtgaccag cgtcgtcgtt tcgcacgatc tcacgtcgat cttcaccatc gccgaccgga
     4441 tcgccatgct gtacaagggg aaggtgcggc tgctcggaac gccccagaca tttcaagcga
     4501 gcgacgatgg agtggtccgc cagttcattc ggggcgaggc ggacgggccc atggagttct
     4561 gatggggcgg acgcgacgcg agttctgggc tagggggtgt cccaagt
//
LOCUS       DASTKS010000902         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1038337, whole genome shotgun sequence.
ACCESSION   DASTKS010000902 DASTKS010000000
VERSION     DASTKS010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038337"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFQ35_18810"
     CDS             complement(<1..277)
                     /locus_tag="VFQ35_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HET9932766.1"
                     /translation="MSQEAPDLPPVPILPPDPAPAAPPLTTGAPPELTPAVPAPPLAA
                     PAPPLVGAPALPLRPPFADGAPPEVVMVPPVVAPPAPLVLAPPDAEVP"
     gene            complement(344..562)
                     /locus_tag="VFQ35_18815"
     CDS             complement(344..562)
                     /locus_tag="VFQ35_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932767.1"
                     /translation="MKLEGAQCPPPGPDIWQVAGKGSWQAIFGQGIAVHDFVIWASTL
                     LYSSSSVEFEALAGTAHPHSVATVSVHC"
     gene            850..1413
                     /locus_tag="VFQ35_18820"
                     /pseudo
     CDS             850..1413
                     /locus_tag="VFQ35_18820"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
     gene            complement(1501..2187)
                     /gene="mutH"
                     /locus_tag="VFQ35_18825"
     CDS             complement(1501..2187)
                     /gene="mutH"
                     /locus_tag="VFQ35_18825"
                     /inference="COORDINATES: protein motif:HMM:NF003458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HET9932768.1"
                     /translation="MLCPPTTEAELLARADALAGESIESVAAKLGVAVPSDLSRHKGW
                     VGNFVERALGASAGSKPEPDFAGLGIELKTLPVDDSGKPFESTFVCTISMSELCDVEW
                     DDSRVRRKLARVLWMPVQGDRRLELCKRRFGAALLWAPDTEELSALRFDWEELAGVVV
                     RLGFEAVTGHAGRFLQIRPKARDSSVRRRGLDADGALISELPRGFYLRQCFTAEILRK
                     NFAIAVAPRA"
     gene            complement(2265..3068)
                     /locus_tag="VFQ35_18830"
     CDS             complement(2265..3068)
                     /locus_tag="VFQ35_18830"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9932769.1"
                     /translation="MGTYGQRCFQEADKLGGLVAKMRLASIAQITSTEAATVSDSPEL
                     VAKLDSALARLRSEFAQPQGAKASPPPPAPAEPPRAAPSVEAALRRSMTAIADLISQR
                     SLFAGDLDATVRRITEAASSTLDIERVSVWFLDEGRTKIVCADLFERGKAEHVSGTEL
                     MASDFAPYFEALAGERTIAAHDANNDPRTRCFSEVYLKPLNIGALLDVPIWAGGGMVG
                     VICHEHVGPSRQWTSDEETFAYVLSSLIALTLEQRTLAHPPTAPTPPTE"
     gene            3216..>3900
                     /locus_tag="VFQ35_18835"
     CDS             3216..>3900
                     /locus_tag="VFQ35_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932770.1"
                     /translation="MPLVSSSRVLVVMLLFGCRAAADDRASNAEVAAALAGSARKLEA
                     APLLPPPEPSAAPQARVVAAVLKPPSQPEPFPPEVPGEARVYAKTRFVWVYPEPDVSR
                     QWIGFLRSGSSAKLRSPKARPGNGCDAFVAIEPRGYVCADGPRATLSADDPVYRAVRP
                     YAAKADSPTPHHYGESLGLPRYLTTPTVAEQRWRERDYTSHQRAVARARSGDVPSWLA
                     GVDFSVPEST"
BASE COUNT          627 a         1327 c         1307 g          639 t
ORIGIN      
        1 gaggaacttc ggcgtcgggc ggggcaagca cgagcggtgc gggcggcgcg acgacagggg
       61 gaaccatgac gacttctggg ggcgctccat ccgcgaatgg cggacgcagc ggcagcgccg
      121 gcgctccgac gagcggtggt gccggcgcag cgagtggcgg tgcagggacc gccggcgtca
      181 gctcgggtgg agcgcccgtt gttagcggcg gcgctgcggg ggcggggtcg ggcggcaaga
      241 tgggcaccgg tggcaaatct ggcgcttcct gcgacacgct gcgtatgcgg ctcgagtcgt
      301 tacttacgga agcgcaggca tgcgagcgta cgggcggcgg ctttcagcaa tgcaccgaaa
      361 ccgtcgcgac cgagtgcgga tgcgccgtcc ccgccaatgc ttcgaactcg acgctgctcg
      421 aggagtacaa gagcgtgctc gcccagataa cgaagtcgtg cacggcgatg ccttgcccga
      481 agatcgcctg ccaagatcct ttgcccgcta cctgccagat gtcgggtccc ggcggcgggc
      541 attgcgcgcc ctccagcttc atgggcccgt gaggggcact tgggcgctct gcgccctcgc
      601 gactgaccat ccgttcgcgg gccctcagcc gaaaacacgt cataaacttg agctctcgcg
      661 agcgggcgcg gcacgatttc gcgaccgatg ctccgttttc gccttctcgt cgcgacgctc
      721 gtagcgtttt cggccctggc gtgcgctcga gagacgccct cggcgcgcaa cccggctgcg
      781 gcgctgcgtc cttgggtcgc gccacccggt tacgattggc cgccgcgcac ggtgcgcaag
      841 gacgaccgca tgcgcccggg cgaacgagcg ctcgagctct cgaagacgag cccgatcacg
      901 tcggcgcatc gggcgcctct cccctccgac actttgatcc ccggcggcga cgagtgcctt
      961 tcgcggctac agcaacaagg cgttcgcttc gagcggttga gcgccgagag gggcgtcgat
     1021 acccccattt tggtgcgcgg cccgatcggt ggggtcgaat tttggtcgct cgggggaccc
     1081 atggtggtgg attgcaggat gggcctcgcg ctcgccgacg tcgcccctga atttgcggcg
     1141 ctcggcgtga cgcgcgcgcg cttctcgggc gcgtacgtgt atcgaaccag ccggcagggg
     1201 cgcctcagcc tgcacgcata tgggctcgcc atcgacattc acgaggtcag agtggcgggc
     1261 gagaccctgt cggtcagcaa ggacttcgag cgcgggcaga gttgcgcgga agatggcccg
     1321 atcctcaacc gactcggttg ccgactgcgc gctctcgggc tgttccgcga gttgctcacg
     1381 cccgactaca acgccgatca tcacgatcac attcacctcg ggctcgcgcc gctccccaat
     1441 ccggagcgcg aggccgcgcc caccccgagg ccagacctgc cgccgagcac ggcgcgctcg
     1501 ctaagcgcgc ggagcgaccg cgatcgcgaa gttctttcga aggatctcgg cggtaaagca
     1561 ctgccggaga tagaacccgc gcggaagctc cgaaatcagc gctccgtccg cgtcaagccc
     1621 acgtcgtcga acgctcgagt cgcgcgcctt cggtcgaatc tgaaggaagc gcccggcgtg
     1681 cccagtgacc gcctcgaacc cgagccggac gacgacgccc gctagctctt cccaatcgaa
     1741 acgcagcgcg gacagctctt cggtgtcggg cgcccacaga agggccgcac cgaaccttcg
     1801 cttgcagagc tcgagccgtc gatcgccttg aacgggcatc cacagcacac gagcgagctt
     1861 tcgacgcacg cgagaatcgt cccattcgac gtcgcaaagc tcgctcatcg agatggtgca
     1921 gacgaacgtc gactcgaaag gcttgcccga gtcatctacg ggaagggtct tgagctcgat
     1981 acccaggccg gcgaagtctg gctccggttt cgatccagcg gaggcaccga gcgcgcgctc
     2041 gacgaagtta ccgacccaac ctttgtgccg actcaaatcg gaggggacgg caacgccgag
     2101 cttcgccgcc acgctctcga tcgactcgcc cgccaaggcg tctgcccgcg cgaggagctc
     2161 ggcttcggtc gtgggcgggc acaacatgcg agcttcatag cgcaaacgga ccccgttcgc
     2221 cgtgtttgcc gggcgctcgg gaggctccgc ggaccacgcg gccgtcactc ggtgggcgga
     2281 gtcggcgcgg tcggcgggtg cgcgagcgtc cgttgctcga gcgtgagcgc gatgagactc
     2341 gaaagaacat aagcgaaggt ctcttcgtcg ctcgtccact ggcggctcgg ccccacgtgc
     2401 tcgtggcaaa tcacccccac catccctccc ccggcccaga tcggcacgtc cagcaacgcg
     2461 ccgatgttca gcggcttcag gtaaacctcc gagaagcagc gcgtccgtgg atcgttgttc
     2521 gcgtcgtggg ccgcgatggt gcgctccccc gcgagcgcct cgaagtacgg agcgaaatcg
     2581 ctcgccatga gctcggtgcc cgacacgtgc tcggccttgc cccgctcgaa caagtcggcg
     2641 caaacgattt tggtgcgccc ctcgtccaga aaccaaacac tgactcgctc gatgtcgagt
     2701 gtcgacgacg cggcctcggt gatgcgccgc accgtcgcgt ccaagtcacc cgcgaagagg
     2761 ctgcgctggg agatgagatc cgcgatggcc gtcatcgaac ggcggagcgc cgcctcgacg
     2821 ctcggagccg ctcggggagg ctcggcgggg gcgggtggtg gagggctcgc cttcgcgccc
     2881 tgtggctgag cgaactccga ccggaggcgc gccagcgccg aatcgagctt ggcgacgagc
     2941 tcgggagagt cgctgaccgt cgcggcctcc gtcgatgtga tttgcgcgat ggaggcgagc
     3001 cgcatcttcg cgacgagccc tccgagtttg tctgcctctt gaaaacaacg ttgaccgtac
     3061 gtgcccattg ctcagctgcc acatgctaac agatggcggg tattttcgcg gctcggagac
     3121 ggagatttca gtcgcgcgca gggctctccc tggcgcccag atagtgcgct cgccacgggc
     3181 gaggtgccca aaactgcgct aacttctccg cgttcgtgcc gctcgtaagt tcatctcgcg
     3241 tcctcgtcgt catgttgctg ttcggctgcc gtgcggcggc cgacgaccgc gcatcgaatg
     3301 ccgaggtcgc ggccgcttta gcgggcagcg cacgaaaact cgaggctgcg ccgttgcttc
     3361 ccccgcccga gccgagcgct gcgccgcaag cgcgcgtcgt ggccgcggtg ctcaagcctc
     3421 cgtctcaacc cgagccgttt ccgccggagg tcccgggcga ggcgcgcgtc tacgcaaaga
     3481 ctcgcttcgt ctgggtttac cccgagcctg acgtgagccg ccagtggatc ggttttcttc
     3541 gaagcgggag ctcggccaag ctccgctctc ccaaggcgcg gcccggcaac ggttgcgatg
     3601 cgttcgtcgc gatcgagccg aggggttacg tgtgcgcgga tggcccgcgc gcaacgttgt
     3661 cggcagacga cccagtatat cgagcggtcc gcccctacgc cgcgaaggcg gactcgccta
     3721 cccctcacca ctacggcgag tcactcgggt tgccacgcta tttgacgaca cccaccgtcg
     3781 ccgagcagcg ctggcgagag cgcgactaca cgtcacatca acgcgcggtc gctcgagcgc
     3841 gctcgggtga cgtgccgtcg tggctcgcgg gcgtcgactt cagcgttccc gagagcacat
//
LOCUS       DASTKS010000903         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_592009, whole genome shotgun sequence.
ACCESSION   DASTKS010000903 DASTKS010000000
VERSION     DASTKS010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592009"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="VFQ35_18840"
     CDS             <1..2284
                     /locus_tag="VFQ35_18840"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HET9932771.1"
                     /translation="VVRKSRASFPALGSFEGATHARNIERFRALDRERLRLTQVEVRA
                     AHAKRLPREQGDAGEVGILRREMSKKRRQLPLRKLFVQASHCIQTIKPVFMMSPISAA
                     QFLEPGGMEFDLLVMDEASQVMPVDALGAVARAKQLVVVGDEKQLPPTEFFQRLGATE
                     DEPTDDEHIGDVQSILGLCLAQGVPSRMLRWHYRSRHHSLIAVSNQEFYDGRLLVPPS
                     PEQASDSRGLKFEYVAEGCFDRGASATNQREAEVVAQRVLEHAKRNPEVSLGVGTFSV
                     AQRDAVLDELERLRKTAPELESFCSRRGEDEFFVKNLESIQGDERDVIFISVGYGKDA
                     FGKLSMNFGPLNNDGGERRLNVLITRARERCVVIASIRADDIDPQRSSARGVVALKHF
                     LAYAEAGSVEGPRPSTVVGSSPFEQQVADAVRALGYAVTTSVGTAGFRIDIAVEDPDR
                     PSKYLLGIECDGVEYAAARNASDRDRLRPQILEARGWQLARVWIVDWLRDPAAQTRRL
                     AELLVAAKERHRLALARTELVDAPAPEGAAASEGPASDASKPIAIEAGAASHALPTAT
                     AATTDAATRAEQAAATPVDYVEAKFAVPERAIQELAVDELASVVEQVLRVEAPIHRDE
                     MARRIATLWGRSRAGSRIQSAVNDALRWLGARGTTTNDGDFFELPNRSVVPRSRAEVS
                     STNLRKAEYLPPSEVRSALLVAIQRNFGADESAVVNEALRLLGFKPAGSQLRQVALGQ
                     LRALQRANAVELRDERYYPS"
     gene            complement(2305..>3251)
                     /locus_tag="VFQ35_18845"
     CDS             complement(2305..>3251)
                     /locus_tag="VFQ35_18845"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9932772.1"
                     /translation="ELARPPLFSCVLLDADTEGVLLFSFNHLIVDAWALAILQRELVL
                     TWHALGRGDVPAFEPLDLSASEHATAMHEELAPIFKRGREAYPPWPPDAYRLPLDHPA
                     PVAPRREGTIVTRRVDAAFLTRIRAATADAGVPLTAALVAAFQYALHRHARRPDVAFT
                     VVRSGRSSRALREVVAYLSYGDTVEGRLVPGETWAALLERAGRIVLDESSERVPYNLI
                     VQPPSMRVVLNVHNFTSNAAETAPFHYEHENRHPYLWPVWDLFFQILPVDGALLVEVL
                     YRDQVLRSETVAQLWGSFERALTALTETPSAEISAEVD"
BASE COUNT          621 a         1047 c         1055 g          528 t
ORIGIN      
        1 ggtcgtgagg aaatctcggg ctagcttccc tgccctcggc agcttcgagg gggccaccca
       61 cgcccggaac atcgagcgtt ttcgagcgct cgatcgcgag cggcttcgac tcactcaggt
      121 cgaagtacgt gccgcccacg cgaagcgttt accgcgtgag caaggcgacg ccggagaggt
      181 cgggatcctg cgacgcgaaa tgagcaaaaa gcgccgacag ttgccgctcc gaaagctctt
      241 cgtgcaagcg agccactgca tccagacgat caaaccggtg ttcatgatga gcccgatctc
      301 ggcagctcag tttttggagc caggcgggat ggaattcgac ctactcgtca tggacgaagc
      361 gagccaagtc atgccggtcg atgcgctcgg cgcggttgct cgcgccaagc aactggtggt
      421 ggtgggcgac gaaaagcagc tcccccccac cgagttcttt caacgcctgg gcgccactga
      481 ggacgagcct accgacgacg agcacatcgg cgatgttcag agcatcctcg ggttgtgcct
      541 cgcgcagggc gtgccttcgc gcatgctgcg gtggcactac cggagccgtc accactcgct
      601 gatcgccgta tccaaccagg aattttacga cggccgactc ctcgtcccgc ccagccccga
      661 gcaggcgagc gactcgcggg ggctgaagtt cgagtacgtg gccgaggggt gcttcgaccg
      721 aggtgccagc gccacgaacc agcgcgaagc cgaggtcgtg gcccaacgcg tgctcgagca
      781 cgccaaacga aaccccgagg tgtcgcttgg ggttgggacc ttctccgtcg ctcagcgaga
      841 cgccgtgctg gacgagctcg agcggctgcg caaaaccgca cccgagctcg agagcttctg
      901 ctcacggcgt ggcgaggacg agttcttcgt gaaaaacttg gaaagcattc aaggtgacga
      961 gcgcgacgtc atcttcatct cggttggata cggcaaggac gctttcggaa agttgagcat
     1021 gaacttcggt ccgctcaaca acgatggcgg cgagcgccgt ttgaacgtgc tcatcacgcg
     1081 cgcccgcgaa cgctgtgtgg tcatcgcgtc gatccgggcc gacgacatcg acccccaacg
     1141 ctcgagcgct cgcggggtgg tcgcattgaa acacttcctc gcctatgcag aggccggaag
     1201 cgtcgaaggg ccccgtccct ctaccgtcgt cggctcttcg cccttcgagc agcaggtggc
     1261 cgacgccgtg cgagcactcg gctacgccgt cacgacctcc gtcggcacag ccggatttcg
     1321 catcgacatc gcggtagaag atccagatcg cccaagcaaa tatttactcg gcatcgagtg
     1381 cgacggcgtg gagtacgccg cggcgcggaa tgcgagcgac cgagatcgcc tgcgtccgca
     1441 aatcctcgaa gcacgcggtt ggcagctcgc acgggtctgg atcgtcgact ggttgcgtga
     1501 tccggcagcc caaacccggc ggctcgcaga gctcttggtg gcagcaaagg agcgccatcg
     1561 cctcgcgctc gcgcgcaccg agctcgtgga cgctccggct ccggaaggcg cggccgcctc
     1621 cgaaggcccg gcgagcgacg cttcgaagcc cattgccatc gaagcgggcg ctgcgtcgca
     1681 tgcgcttccg accgcaaccg ctgcaacgac cgacgcggcg acgagggccg aacaagccgc
     1741 agctacgccg gtcgactacg tcgaggcgaa gttcgcggtt ccggaacgcg ccattcaaga
     1801 gcttgccgtc gacgagctcg cgagcgtggt ggagcaggtt cttcgagtcg aagcgcccat
     1861 ccaccgggac gaaatggcgc gccgaatcgc gacgctttgg ggacgttcgc gggccggctc
     1921 gcgcattcaa agcgctgtaa acgacgcgct gcgctggctc ggcgctcgcg gcacgacgac
     1981 gaatgacggc gacttcttcg agctcccgaa ccgaagcgtg gtgccccgca gtcgcgccga
     2041 ggtgtcatct accaacctcc gaaaagcgga atatctgccc cccagtgaag tacgaagcgc
     2101 gctgctcgtc gcgattcaac ggaacttcgg ggccgacgag agcgccgtcg tgaacgaggc
     2161 gctgcgcctg ctcggcttca aaccggcggg aagccagctt cggcaagttg ccctgggtca
     2221 actgcgagct ctgcagcgtg ccaacgcggt ggagctgcgc gacgaacgct actacccgag
     2281 ctgatagccc gcccagcgcc caagtcagtc gacttcagca gaaatctccg ccgaaggggt
     2341 ctccgtcagc gcggtgagtg cgcgctcgaa ggagccccac agctgcgcga ccgtttctga
     2401 ccgcagtacc tggtctcgat agagcacctc gacgagcaac gctccgtcca cggggaggat
     2461 ctgaaaaaag aggtcccaaa cgggccagag gtaaggatgc ctgttctcgt gttcgtaatg
     2521 aaacggcgcc gtttcggcgg cgttgctcgt gaaattatga acattgagta cgacccgcat
     2581 ggacggcggc tgtacgatca aattgtacgg cactcgctcg ctcgattcgt ccaacacgat
     2641 tcggccagcg cgctccagca gggccgccca agtttcgccg gggacgagcc gcccttcgac
     2701 cgtatcgccg tagctcaagt acgcgaccac ctcccgcagt gcgcgcgagc tacgtccgct
     2761 acgaacgacc gtgaacgcca cgtcgggccg ccgagcgtgc cgatgcaaag cgtactgaaa
     2821 cgctgccacc aacgccgcgg tcaggggcac tccggcatca gcggtggccg cgcgaatgcg
     2881 cgtaaggaat gcggcgtcga ctcgtctcgt aacgatggtt ccctctcttc gcggcgcgac
     2941 gggcgccgga tggtcgagag gtagccggta ggcgtcgggg ggccaaggcg gatacgcttc
     3001 tctcccgcgc ttgaagatgg gcgcgagttc ttcgtgcatt gcggtcgcgt gctcggaggc
     3061 gctcaggtcg agcggctcga acgcgggaac gtcaccgcgg ccgagcgcgt gccatgtgag
     3121 aacgagttcg cgctgcagaa tcgcgagcgc ccacgcgtct acaatcaggt gattgaagct
     3181 gaagaggagc acgccttcgg tgtccgcatc gagtagtacg caagaaaata gcggcggccg
     3241 cgcgagttcg a
//
LOCUS       DASTKS010000904         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1709016, whole genome shotgun sequence.
ACCESSION   DASTKS010000904 DASTKS010000000
VERSION     DASTKS010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709016"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFQ35_18850"
     CDS             complement(<1..873)
                     /locus_tag="VFQ35_18850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9932773.1"
                     /translation="MRERIAIVTSSYPREPGDASGHFVQSEARALVAAGHDVTVFAAG
                     RPREEAGSPRVVWLADGGAAGFPGLKARLRAMPLRWFGLAAWLTRVRAALDARGPFER
                     IVAHWLVPAALPLLALDAPSARLELVVHGSDARVLARLPRPLARSLLRTLLARDASLR
                     CVSQELAELVQSLAGQPLADRLRVEPAPIDVSGAPSREQARKELGLRAEARLGVVVAR
                     LIAEKRVDVALRAALRVEGLDVAVIGDGPERPALSRAFPSVRFTGQLARHDALRWIAA
                     SDVVLSASRNEGAPS"
     gene            complement(876..1223)
                     /locus_tag="VFQ35_18855"
     CDS             complement(876..1223)
                     /locus_tag="VFQ35_18855"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET9932774.1"
                     /translation="METRVKPTRVKAPHGARSLEITWGDGHRSSIPHEILRGYCPCAS
                     CQGHSGTIKFQEGGNLEVRELEQVGNYALSFRWGDGHEAGIYTFRYLRALGDMVDAQG
                     AEAVKALGELPRL"
     gene            complement(1237..1839)
                     /locus_tag="VFQ35_18860"
     CDS             complement(1237..1839)
                     /locus_tag="VFQ35_18860"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET9932775.1"
                     /translation="MTTTLPPFTHGFELTLPEWTRELLTTSPELFRTSEDRMRLTLHA
                     ARENFQRDTGGPFAAAVFEEQSGRLVSLGVNRVVPQSCSSAHAEVMALSLAQRRLGTF
                     DLGAKGLPSHQLVVNWRPCAMCFGAIPWSGVRSLVIAGGGPAIERLTGFDEGPIHPEW
                     AAELERRGIHVTEGVLESDSWRLFEDFAKSGRLVYNGRQG"
     gene            complement(1844..2332)
                     /locus_tag="VFQ35_18865"
     CDS             complement(1844..2332)
                     /locus_tag="VFQ35_18865"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9932776.1"
                     /translation="MTADEIVRTLGLVPHPEGGSYREVFRSELRLPSATHDGERSAST
                     AIYFLLKSGEFSAFHRVRSDEGWHHYAGDPLELTLLTPERVEVVRLGKDIVNGERPFA
                     MVRAGVWQAARPTAGAEGFTLVGCTVAPGFDFSDFEMPTRAELIAQLPEHRETIVPLT
                     RA"
     gene            complement(2336..2863)
                     /locus_tag="VFQ35_18870"
     CDS             complement(2336..2863)
                     /locus_tag="VFQ35_18870"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9932777.1"
                     /translation="MPRRAELGVPAAHTAALFLTMRVAEGFLWPDPFADPKARSWFRH
                     YGEAFGKPPLFDPHERAFEWDHDRWTINVLGHGLLGSELYYRPRRCGASPLGAFAFAM
                     GATVAWEYAFEGNGVRPSALDLVYTPLAGAVLGEGRYWGWTLARQIRSPVVRGVFRVV
                     LDPLGEIERSLGSVC"
     gene            3073..3327
                     /gene="rpsU"
                     /locus_tag="VFQ35_18875"
     CDS             3073..3327
                     /gene="rpsU"
                     /locus_tag="VFQ35_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET9932778.1"
                     /translation="MPNPSEAVTCRPLEVQVGDKGIERAIKHLKRRMAAEGILRELKR
                     RRHYMKPSVKRRKKMSEAARRRRKRLRADEQQRGGPGGML"
     gene            3465..>3838
                     /locus_tag="VFQ35_18880"
     CDS             3465..>3838
                     /locus_tag="VFQ35_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932779.1"
                     /translation="MPSKPEWPLPFRLRHVALLGLLGGVASWVAPRAEAAWQIHALGS
                     ALADYSLCMVGPTGPETIRDDGEAFRRLVRRRLVGAAPDESPFARCAHAARELTSSVD
                     VERAHQARAQNFVEYGLEPAQAG"
BASE COUNT          651 a         1255 c         1354 g          578 t
ORIGIN      
        1 gctgggcgcc ccttcgttgc gcgacgcgga gaggaccacg tccgacgcgg cgatccagcg
       61 cagcgcgtcg tggcgcgcga gctgaccggt gaagcgcacg ctcgggaaag cgcgcgaaag
      121 cgccgggcgc tcggggccat cgccgatgac cgcgacatcg agcccctcga cgcggagcgc
      181 ggctcggagt gccacgtcga cgcgcttttc ggcgatcaag cgggcaacga cgacgccgag
      241 gcgcgcttcc gctcggagcc cgagctcttt tctcgcttgc tcgcgtgacg gagcaccgct
      301 gacgtcgatg ggcgcgggct ccacccgcag ccggtcagcg agcggctggc cggcgagcga
      361 ctgaacgagc tcggcgagct cttgcgaaac gcaacgtaag ctcgcgtcgc gcgcgagtag
      421 tgtcctgagc agcgagcgag cgagcgggcg gggcagcctc gcgagcacgc gcgcgtcgga
      481 gccgtgtacg acgagctcga gccttgcgct cggggcatcg agggcgagca atgggagcgc
      541 cgccggcacg agccagtgcg ccacgattcg ctcgaagggc ccgcgtgcgt cgagcgcggc
      601 tcgtacccga gtgagccacg ccgcgaggcc gaaccagcgc aacggcattg cgcgtagccg
      661 cgccttgagc ccaggaaaac cagccgcccc gccatcagcg agccaaacga cgcgcgggga
      721 gccagcttct tcgcggggac gcccggccgc gaacacggtg acgtcgtgcc cggccgccac
      781 gagagcgcgt gcctcgcttt ggacgaagtg gcctgacgca tcgcccggct cgcgcgggta
      841 cgagctcgtg acgatcgcga tgcgctcgcg cacgctcaca agcgaggaag ctcgccgagc
      901 gctttgaccg cctcggcgcc ttgtgcgtcg accatgtcgc cgagcgcgcg caggtagcga
      961 aacgtgtaga tccccgcttc gtgcccgtcg ccccagcgaa acgagagcgc gtagttcccg
     1021 acctgttcga gctcgcgcac ctcgaggttg ccgccttctt ggaacttgat cgtgccggaa
     1081 tggccttggc acgaggcgca ggggcaatag ccgcgcagga tctcgtgggg gatggaggag
     1141 cgatgtccgt cgccccaggt gatttcgagg ctgcgtgcgc cgtgcggcgc tttgacgcga
     1201 gtcggtttga cgcgagtttc catggtgctc gaggagtcag ccttggcggc cgttgtaaac
     1261 caggcgtccg ctcttcgcga agtcttcgaa caggcgccag gagtcgctct ctagcactcc
     1321 ctcggtcacg tggatgccgc ggcgctcgag ctcggccgcc cattctgggt gaatcggccc
     1381 ctcgtcgaac ccggtcaggc gctcgatcgc aggcccgcca ccggcgatga cgagtgaacg
     1441 gacgccggac caggggatcg ctccaaagca catggcgcat gggcgccagt tgacgactag
     1501 ttggtgggac ggcagtccct tcgcaccgag gtcgaaggtg ccgaggcggc gctgcgcgag
     1561 tgaaagagcc attacctcgg cgtgcgccga agagcagctt tgcgggacga cgcggttcac
     1621 gccgagcgag accaagcgac cgctttgctc ctcgaacacc gcggccgcga agggaccgcc
     1681 ggtgtctcgc tggaagttct cgcgtgcggc gtgcagggtg agccgcatgc ggtcctcgct
     1741 cgttcgaaag agctctggcg aggtcgtgag cagctcacgg gtccactccg gaagcgtgag
     1801 ctcgaagccg tgcgtgaagg ggggaagggt cgtcgtcatc aaatcacgcg cgggtgaggg
     1861 gaacgatggt ttcgcgatgc tcgggcaact gcgcgatgag ctcggcgcgc gtcggcatct
     1921 cgaaatcgct gaagtcgaag ccaggcgcca ccgtgcaacc cacgagagtg aagccttcgg
     1981 cgccagcggt cgggcgcgca gcttgccaga cgccggcgcg aaccatcgcg aacggacgct
     2041 ccccgttcac gatgtccttg ccgagcctga cgacctcgac gcgctccgga gtgagcagcg
     2101 tgagctcgag gggatcgccc gcgtagtggt gccagccctc gtccgagcgc acccgatgaa
     2161 aggccgaaaa ttcacccgac ttcagcagga agtagatggc ggtcgaggcg ctgcgctcgc
     2221 cgtcgtgcgt ggccgacggg agcctgagct cggagcggaa cacttcgcgg tacgagccgc
     2281 cctcggggtg cggaacgagc ccgagcgttc ggacgatctc atcggcggtc atgattcagc
     2341 acacgctgcc gagggagcgc tcgatttcgc cgagggggtc gagcaccacg cgaaacaccc
     2401 cgcgcacgac tgggcttcgg atttggcgcg cgagcgtcca gccccaatag cggccctcgc
     2461 cgagcacggc accggcgagc ggcgtgtaaa ccaaatcgag ggccgacgga cgcacgccat
     2521 tgccttcgaa agcgtactcc cacgcgacgg tcgcgcccat cgcaaacgcg aacgcgccga
     2581 ggggcgatgc gccacaacgt cgaggcctgt aatagagctc cgacccgagc aggccgtggc
     2641 ccagcacgtt gatcgtccaa cgatcgtggt cccattcgaa agcacgctcg tgaggatcga
     2701 agagcggcgg tttgccgaat gcttcgccat agtgtcgaaa ccaagaacga gccttgggat
     2761 ccgcgaaggg gtccggccac aggaaccctt cggcgacgcg catggtgagg aagagcgcgg
     2821 cggtgtgtgc ggccgggacg ccgagctccg cgcggcgcgg cacctcctct gagcgggcct
     2881 ccgccgctgg agcgttcgga gccgccagcg gcagatcgaa gaagaccgga ggcgttaacg
     2941 cgaagatgct ttggggttac cagagttacg ggtcgtttgc tgtaggcccg acggccgctt
     3001 cgcctgggcc tctttctcga gcgtctgtgt cagcttgcgt tgcgaacgga ttcatgacaa
     3061 acgcaggctc ccttgccgaa ccctagcgaa gcagttacgt gtcggcccct cgaagtccaa
     3121 gtcggcgaca agggcatcga gcgagccatc aagcatctca agcgccgcat ggcggccgaa
     3181 ggcatccttc gggagctcaa gcgccgccgc cattacatga agccgtcggt gaagcgccgc
     3241 aagaagatga gcgaggccgc ccgccgtcgt cgcaagcgct tgcgcgcgga cgagcagcag
     3301 cgcggcggac ccggcggaat gctgtaggga ttttggccgc atgcgcccgc tgagaatagc
     3361 gtggcagcgt gccgccacat tctgccctta gcgcgctcga ggctcgtcgg acgaagccgc
     3421 tgcgcgcttc cgcaccgaag cgtacccaac cgccagcgcc agtcgtgcct tcgaagcctg
     3481 aatggccttt gccgttcagg cttcggcatg tggcgctgct gggtcttttg ggcggagttg
     3541 cgtcttgggt cgcgccccgc gccgaagcgg cgtggcagat ccatgcgctc ggaagcgctc
     3601 tcgccgacta ctcgctgtgc atggtgggcc cgacgggccc ggaaaccatc cgcgatgacg
     3661 gagaagcgtt tcgccgtctc gtgcgccgtc gtttggtcgg cgcggcgccg gacgagtctc
     3721 cctttgcccg ctgcgcgcat gcggcgcggg agttgacgtc cagcgtcgac gtcgagcgcg
     3781 ctcaccaggc gagggcgcaa aatttcgtcg agtacgggct cgagccggcg caggcagg
//
LOCUS       DASTKS010000905         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1038637, whole genome shotgun sequence.
ACCESSION   DASTKS010000905 DASTKS010000000
VERSION     DASTKS010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038637"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFQ35_18885"
     CDS             <1..743
                     /locus_tag="VFQ35_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9932780.1"
                     /translation="LKELCFEYLPGRPVLHKVNLRVEAGQTLALVGHTGSGKSTIVSL
                     VAKLVLPTTGQLFIDGREIRNISSASLHSQIACVTQENFLFKGSVLENIRVGRRDATD
                     EDVRSALAALDVLDLIEDLPRGLETQVGERGSGLSLGQRQVVCFARAMLRDPKILLLD
                     EATSSVDTMTEARIQDALLRLLAGRTSIVVAHRLSTIRHAGQVVVLDHGRIIERGTHP
                     ELLAAGGKYAHMYRQFVSAADPAEAKSA"
     gene            918..2159
                     /locus_tag="VFQ35_18890"
     CDS             918..2159
                     /locus_tag="VFQ35_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9932781.1"
                     /translation="MRDTASVQGKVDSRAMREINRSIVLDIIRRGGKVSRTDLAKRSA
                     LTKPTVSAIVDDLLARGIVQEVGYGRTMGSGGRRARLLEFNDASAAYLGIRFGVRTTQ
                     VGIADARGGLRVVREIPSAPSEPERAVREALALVDSLLAEANLPRERLQSAGVAIPAL
                     VSRSGVVELAPNLNFKQLPLRELVQEGLGLPVVVMNDTHAGALAEGRCGAARGVRSFV
                     WVQVGTGVGAGIVIDGKVFHGHVGFSGEIGHCPVIADGPVCSCGLRGCLEAVASGWAI
                     RRAADAAVASGEASLLAAPGCDREARAVAEAARAGDAVACRILSDVGAYLGMGISTLV
                     NLLNPEMIVLGGGVMSAPDHIIEAAQTSMAKHALRGADVRIVPGMLGERVGLVGALLG
                     AMDLSVRSLRVVAGREHAALE"
     gene            complement(2165..>3159)
                     /locus_tag="VFQ35_18895"
     CDS             complement(2165..>3159)
                     /locus_tag="VFQ35_18895"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: protein motif:HMM:NF016857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HET9932782.1"
                     /translation="TVAASRDTADGRLHFTPANLVSMLHRSLEAETTTRVLRTIFAAD
                     ERFCVHEPLPAHPEFSDEGAANHTRLVTSRGSLNLFAWGRAPDVALTPKRFPARQSRS
                     ASEAVIRLHQLPDERVLTWQQDPDGIDAGAFHTDVLAVGSGSFFMMHERAFVHCEAFT
                     AELRARLGDELAVVTARETELPVADAVGCYPFNSALIPLSNGDLIVLAPEESRANPNA
                     AAFLDRVVSEPNPVSRVVYVDVNDSMRNGGGPACLRLRVPLSDAERSALRGRVLFDSA
                     LDAQLTSWVSRHYRDRLAQRDLLDPAFLRESREALDALTQLLKLGSIYDFQRSP"
BASE COUNT          507 a         1018 c         1081 g          553 t
ORIGIN      
        1 agctcaagga gctgtgcttc gagtacctgc ctggacgccc ggtcctccac aaggtcaatc
       61 tgcgggtcga ggctgggcag acgctcgcgc tggtcggcca cactggcagc ggcaagagca
      121 cgatcgtcag cctggtcgcg aagctcgtct tgccgacgac ggggcagctt ttcatcgacg
      181 gccgggagat ccggaacatt tcgagcgcgt cgctgcattc gcaaatcgcg tgcgtcaccc
      241 aagagaactt cttgttcaag ggtagcgtcc tcgagaacat tcgagtgggg cgccgcgatg
      301 ccaccgacga agacgtgcgg agcgcgctcg ccgcgctcga cgtgctcgac ctcattgagg
      361 acctaccgcg cggtctcgag acgcaagtgg gggagcgagg ctcggggctg tcgctcggtc
      421 agcgacaggt cgtgtgcttc gcgcgcgcga tgctgcgcga cccgaagatc cttctgctcg
      481 acgaggcgac gagctcggtc gatacgatga ccgaagcccg catccaggac gccttgctgc
      541 ggctcctggc cggccgcacc agcatcgtcg tcgctcatcg gctcagcacg atccgacacg
      601 ccggtcaggt ggtcgtgctc gaccacgggc gaattatcga gcgtggtact cacccggaac
      661 ttttggcggc gggcggaaag tacgcccaca tgtaccgtca gttcgtgagt gctgctgacc
      721 ccgccgaagc caaaagcgcc tgaaatcgtt cgtatttccc tcgtaatccg gcaccggttc
      781 caggcttcac tcccgggggg gatccgcccc agtgtcgggc tcggccaggt tcgaaaagat
      841 cgaatgcatt ttcctgggcc gttcgggtcg cctcaaacga ctcccattct gccacgccga
      901 ataacgaggt taccccgatg cgagacactg caagcgttca gggaaaagtc gacagccgcg
      961 cgatgcgcga aatcaaccgc tcgatcgtgc tcgacatcat ccgacgtgga gggaaggtct
     1021 cgcgcaccga tctggcgaag cgttcagccc tgaccaagcc cactgtgtcc gccatcgtcg
     1081 acgacctctt ggctcgcgga atcgtccagg aggtcgggta tggaaggacg atgggcagcg
     1141 gcggccggcg cgcccgattg ttggagttca acgacgcctc tgctgcctat ctcggcattc
     1201 gcttcggcgt gcgcacgacc caggtgggga tcgccgacgc gcgcggtggc ctgcgcgtag
     1261 tgcgtgagat cccgagcgct ccctccgagc cggagcgcgc cgtgcgagag gcattggcgc
     1321 tggtcgacag cttgctcgcg gaagcgaatt tgccgcgcga gcgcctacag tcagccggcg
     1381 ttgcgatccc ggcgctggtc tctcgcagcg gcgtggtgga gctcgcgccg aatctgaatt
     1441 tcaagcagct gccactgcgc gagctggtgc aagagggtct cggtttgccc gtcgtggtga
     1501 tgaacgacac gcacgccggt gcgctcgccg aagggcgttg cggcgccgcg cgcggggttc
     1561 gctccttcgt ttgggttcaa gttggcaccg gggtcggcgc cggcatcgtc atcgacggaa
     1621 aggtgttcca cggccacgtc ggcttcagcg gtgagatcgg gcattgcccg gtgatcgcgg
     1681 atggcccggt gtgcagctgt ggtctccgtg gctgcctcga ggcggtcgcg tcgggctggg
     1741 ccattcgtcg cgctgccgac gcggcggttg cgagcggcga agcgtcactg ctcgctgcgc
     1801 cagggtgcga tcgcgaagct cgcgccgtcg ccgaagcggc ccgcgccgga gacgccgtcg
     1861 cttgtcgcat cttgtcggac gtcggcgcgt acctcggcat gggtatttcg accctggtca
     1921 acctcctgaa tcccgagatg atcgtgctcg gcggcggcgt catgagcgcg cccgatcaca
     1981 tcatcgaggc ggctcaaaca tcgatggcca agcacgcctt gcgcggcgcc gatgtgcgca
     2041 tcgtaccggg catgttgggg gaacgtgtcg gcctcgtcgg cgcgcttctc ggggcgatgg
     2101 acctctcggt gcgctcgctg cgcgtcgtgg ccggccgcga gcacgccgcg ctcgaataac
     2161 gccgtcacgg cgagcgctgg aagtcgtaaa tcgagccgag cttcaagagc tgagtcagcg
     2221 cgtcgagcgc ctcccggctc tctcgcaaga acgcggggtc gagcaagtcg cgctgcgcga
     2281 gccggtcgcg gtagtggcgg ctcacccagg aagtgagctg cgcgtcgagc gccgagtcga
     2341 agagtacgcg gccgcgcagc gcgctccgtt cggcatcgct gagcggcact cgcaggcgta
     2401 agcacgcagg tccgcctccg tttcgcatcg agtcgttcac gtcgacgtac acgactcgcg
     2461 agacgggatt gggctcgctc acgacgcggt ccaagaacgc ggccgcgttc ggattcgcgc
     2521 ggctctcttc gggcgcgagt acgatcaagt cgccgttcga gagcggaatc agcgccgaat
     2581 tgaatgggta gcaacccacc gcgtccgcga cgggcagctc tgtttcgcga gcggtcacca
     2641 cggcgagctc gtccccgagc cgggcgcgta gctccgccgt gaacgcttcg cagtgcacga
     2701 aggctcgctc gtgcatcatg aagaagctcc cgctgccaac cgccagcacg tcggtgtgga
     2761 acgcgcccgc gtcgatgcca tccggatctt gttgccacgt caggacgcgc tcgtccggga
     2821 gctgatgcaa gcggatcacc gcctcgctcg ctgaccgtga ttgtcgtgcc gggaagcgct
     2881 tcggtgtgag tgctacgtcc ggcgcgcgac cccacgcgaa caggttgagc gagcctcgcg
     2941 acgttacgag gcgtgtgtga ttggccgcgc cttcgtccga aaactccggg tgcgccggca
     3001 aaggctcgtg cacgcaaaag cgctcgtcgg ccgcgaagat tgtccgcagc acgcgcgtcg
     3061 tcgtttcggc ttcgaggctc cggtgcagca tcgaaaccaa attggcgggg gtgaagtgca
     3121 agcgaccgtc tgccgtgtcg cggctcgcgg cgacggtcg
//
LOCUS       DASTKS010000906         6252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1709407, whole genome shotgun sequence.
ACCESSION   DASTKS010000906 DASTKS010000000
VERSION     DASTKS010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709407"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFQ35_18900"
     CDS             <1..548
                     /locus_tag="VFQ35_18900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9932783.1"
                     /translation="DGASVARELERFVAHVGGVDGWVNAAGVHRAALLVSAEESAIEE
                     QLDVNLLGTILCTRAVLPQMLRQRSGVVLNVSSVASVYPVRGGATYAASKAGVEAFTR
                     AIALEYGRKGIRAVCIRPGPIATNMLAPTQAISETPIAERTALQRLGSPDDVASMAAF
                     LLSDEGSFVTGSVHAVDGGFS"
     gene            545..1171
                     /locus_tag="VFQ35_18905"
     CDS             545..1171
                     /locus_tag="VFQ35_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9932784.1"
                     /translation="MIEDALRRRRSVRRFKADPVPEELVVRLLEGAITAPSASNKQPW
                     KFLVVENRSLITRMADAVRTATALIAEHIPEESRAAFVAYGDYFTRFEAAPLVIVPIC
                     RPLTLLSNLTGESLPDARRACVQQLEATSGLVSTSLALGNILLLAEELGLGASGMTGP
                     LVARAELKQLLDVPNTWDIVALVPIGFPAEFPEPTERKSMKFVSRWYR"
     gene            1168..1566
                     /locus_tag="VFQ35_18910"
     CDS             1168..1566
                     /locus_tag="VFQ35_18910"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9932785.1"
                     /translation="MTDESVFERVRASIAESLAVPRDEIKLDSRMVDDLGADSLDLVD
                     IIFTLERKFGINVRETEFNFLTRLDFSSPEVMRDGYLTREVVERLGTWLRGVRESEER
                     DKITPRSLFSMITVESICTVVERRTGGAPP"
     gene            complement(1571..2134)
                     /locus_tag="VFQ35_18915"
     CDS             complement(1571..2134)
                     /locus_tag="VFQ35_18915"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9932786.1"
                     /translation="MDATKLLQTQHQEVKSLFEKLESQGESPDPAVFEELARNLVAHD
                     AIEREIFYPACEQAMGMTHHLAEALVEHGYVEFGLYQADLAVGEDSFEAKLNVLKEMV
                     LHHVAEEEDEFFPEARAAIESSKLSELGDQMQTRFEEVKRSDIRELTRANLLQVMSGA
                     LKTQPGKASPKGQSVSNGRTNERNATR"
     gene            complement(2170..2463)
                     /locus_tag="VFQ35_18920"
     CDS             complement(2170..2463)
                     /locus_tag="VFQ35_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932787.1"
                     /translation="MLKQALRIGFAFVLVAATLGGCSDAKNTITCADVCSRYKDCFDS
                     DYDVSSCTDKCKSRAGSDDDKQSQLNQCDACIDDKSCTAAAFGCATECVGIVP"
     gene            2874..3386
                     /locus_tag="VFQ35_18925"
     CDS             2874..3386
                     /locus_tag="VFQ35_18925"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9932788.1"
                     /translation="MATMVSATQSTTERFIEILKNFDTAILLTHASDGSLHGRPMAFA
                     DVESDGTLWFLTRIDSPKIEELSADPRAFVTCQDSNEYATLSGTCEVSKDARRLDEVW
                     KESYKVWFDGKDDPRLVLLRVTPESGEYWDNSGSKGVRYAFRAVAAYVQGKKMDEKAD
                     DPTQHAKVAL"
     gene            3421..4023
                     /locus_tag="VFQ35_18930"
     CDS             3421..4023
                     /locus_tag="VFQ35_18930"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9932789.1"
                     /translation="MDAKLIVYAALAALGCSHRGDTPESESAPRTTPTTVVATRANEQ
                     YVFESPPDAPTKQKRLPNESTASKRERAPEVGEARAKSNFVEPGTHLDDSGNTVTIPP
                     ADMTDEAAPSADSSEAQPTAFDQGESERDRTITSQIRRALMGESSLSFAAKNVAVVTK
                     DGNVTLRGTVKTAAERSTVDNFARRIAGERAVENRIEVTP"
     gene            4035..4586
                     /locus_tag="VFQ35_18935"
     CDS             4035..4586
                     /locus_tag="VFQ35_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET9932790.1"
                     /translation="MANKSVIGIVETQIQAESIVGSLQNAGFSSNSISALFPDRRGTA
                     DFAHEHNTKAPEGAVAGVAGGGVVGGTLGLLAGIGALAIPGLGPFIAAGPLMAALSGA
                     AAGAAVGGVTGALVGLGIPEIEAKKYEGKIRGGNIIVAVHVDDSKERERATEILRRGG
                     AHDVTSTSESNVPREESRTTTQH"
     gene            4779..5231
                     /locus_tag="VFQ35_18940"
     CDS             4779..5231
                     /locus_tag="VFQ35_18940"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9932791.1"
                     /translation="MPNSTPPPFDGRRRSQPALSDEARANELLAEEASVEIRTLIRYE
                     LDLAREQTAEAVQKAHYAALIGASAVALSVLALSSITVALLLALGASAVPALVVAALM
                     TVTGAVGAGYMYFVLPHSPLEYARALLKTRLGEVRREIDAHKASPQRS"
     gene            complement(5330..6085)
                     /locus_tag="VFQ35_18945"
     CDS             complement(5330..6085)
                     /locus_tag="VFQ35_18945"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET9932792.1"
                     /translation="MVRQRTLATLAGSLALMMLQCAEHEPAKNADAANMQTASTPGAM
                     GNDTNPPTGPAPDAVATTATEQRKDTTSDSTSANSNKPTDDAPPSALSDAQIAGITDG
                     ANSAEIEQAKLARSKSKNQKVLQFASMMITHHGDAQKKQAKLNITSAESPLSAQMTTD
                     ANQILTSLKDKNGMDFDRAYMRSQVDEHQALLDALNTRLLPSVSSPELKAYLQEIKTR
                     VEQHLEAARTTESTFGTTPNTTNRPSNAASSVK"
BASE COUNT         1086 a         1991 c         2001 g         1174 t
ORIGIN      
        1 gtgacggcgc cagcgtggcg cgtgagctcg agcggttcgt ggcgcacgtg ggcggcgtcg
       61 acggctgggt caacgcggcc ggagtgcatc gcgcggcgct gctcgtgagc gcggaagaga
      121 gcgcgatcga ggagcaactc gacgtgaacc tgctcggtac catcttgtgc acgcgcgcgg
      181 tattgccgca gatgctgcgc cagcggagcg gagtcgtgct caacgtgagc tcggtcgcga
      241 gcgtctatcc cgttcgaggc ggagcgacct acgccgccag caaggcgggc gtcgaagcgt
      301 tcactcgagc aatcgccctc gagtacggca gaaagggaat tcgggccgta tgcatccgcc
      361 ccggccccat cgcgacgaac atgctcgccc cgacgcaagc catcagcgaa actcccatcg
      421 ccgaacgaac cgctctgcag cggctcgggt ccccagacga cgtcgccagt atggccgcat
      481 ttctcttgtc cgacgaaggc agcttcgtga caggctccgt ccacgctgtc gatggcgggt
      541 tctcgtgatt gaagacgcgt tgcggcgccg ccgcagtgtc cgtcgcttca aagccgaccc
      601 ggtgccggaa gagctcgtgg ttcgactgct ggagggcgcc atcactgcgc cctcggcgag
      661 caacaagcag ccatggaagt tcctggtcgt cgagaatcgg agcctcatca cccgcatggc
      721 cgacgcggtg cggacggcaa ccgcgctcat cgcggagcac atccccgagg agagccgcgc
      781 cgctttcgtc gcttacggcg actacttcac ccgcttcgaa gccgccccgc tcgtcatcgt
      841 acccatctgt cggccgctca ccctgctctc gaacttgacc ggcgagagct tgccggacgc
      901 gcggcgtgcc tgcgtccagc aactcgaggc gacttcgggg ctcgtcagca cctcgctcgc
      961 gctcgggaac attctgctcc tcgccgagga gctcgggctc ggcgcttcgg gtatgaccgg
     1021 tccgctcgtc gcgcgtgccg agctcaaaca gttgctcgat gtcccgaata cctgggatat
     1081 cgtcgccctg gtgcccatcg gtttccccgc cgaatttccg gagccgacgg agcgcaagtc
     1141 gatgaagttc gtgagccggt ggtaccgatg accgacgaat ccgttttcga gagagtgcgt
     1201 gccagtatcg cggagagcct cgccgtcccc cgcgacgaga tcaagctcga cagtcgcatg
     1261 gtggacgacc tcggcgcgga ctcgctggat ctcgtcgaca ttatcttcac gctggagcgc
     1321 aagttcggca tcaacgtgag agaaaccgag ttcaacttcc tcacccggct cgacttctcg
     1381 agccccgagg ttatgcgcga cggctacctc acgcgggaag tggtggagcg cctcggcacg
     1441 tggctccgcg gcgtgcgaga gtcagaagag cgcgacaaga tcacgccgcg ctctctgttc
     1501 tcgatgatca ccgtcgaatc catctgcacg gtcgtcgaac gacgcacggg cggcgctccg
     1561 ccctgaccgc tcagcgcgtc gcgttgcgct cgttcgtgcg gccgttgctc acgctctgac
     1621 ctttgggcga agccttgcca ggctgcgtct tgagggcgcc gctcatcacc tgcagcaggt
     1681 tcgcgcgcgt gagctcgcgg atgtcactcc gcttcacttc ttcgaaccgc gtttgcattt
     1741 ggtcgccgag ctcactcagc ttgctggact cgattgcggc acgcgcttcc gggaagaact
     1801 cgtcctcttc ctcggcgacg tggtgcagca ccatctcctt caagacgttc aatttggcct
     1861 cgaaagagtc ctctccaacc gccaggtcgg cttgatagag cccaaactcg acgtagccgt
     1921 gctcgaccag cgcctcggcg aggtggtgcg tcatacccat cgcttgctca caggcggggt
     1981 agaagatctc gcgttcgatg gcgtcgtggg caacgaggtt tcgggccagt tcctcgaaga
     2041 cggcggggtc gggcgactcg ccctgcgatt ccagtttctc gaacagggat ttgacttcct
     2101 ggtgctgggt ctggagaagt ttggttgcgt ccatggtctt gctcattctc tcgttcaggt
     2161 tgttcgaatt cagggcacga ttccgacgca ctcggtagcg cagccgaaag ccgcggcggt
     2221 gcacgacttg tcgtcgatgc aagcgtcgca ctggttgagc tgcgattgct tgtcgtcgtc
     2281 gctgccggca cgcgacttac acttgtcggt gcaagacgat acgtcgtagt cggagtcgaa
     2341 gcagtctttg tagcggctgc atacgtccgc gcaggtgatc gtgtttttcg cgtcgctgca
     2401 gccgccgagg gtcgccgcga cgagaacgaa ggcaaagcca atccgaagag cttgcttaag
     2461 cattgggtgt ttccgttggt gctgaggcga actcactcgt tcgcgacagt cgcacaaggc
     2521 gtatcgaatg cgtgagttag caaggcacgt gcctaggcct gcgctcgtgg ctttcgagcg
     2581 agctactcgt acgctgactc ccgatcggaa cgaccgggga acgtactttc acgtgatgag
     2641 cagcgcaccc gtgcctcagc ggagcagcgc gccctgaacg gctcggctcc gcagccctcg
     2701 tggatgcagc ggcgtcgcct cgctgaggca gcgtagcgcg tcgctgcgaa accgaaagga
     2761 ctcagtggct cagtgactcg cccacaatcg aggctcgcgc gccgaaaaag cctaaatcac
     2821 gactatccat cgctcgccac ggcagcggca caccgattgc ttaatcgagt tccatggcaa
     2881 cgatggtatc ggcgacccaa tcgacgaccg agcgcttcat cgagattctc aagaacttcg
     2941 atacggcaat tttgctgacg catgcgtccg atgggtcgct tcacggccgt cccatggcct
     3001 tcgccgacgt cgagtccgac gggacgctct ggttcctcac gcgcatcgac tcaccgaaaa
     3061 tcgaagagct ctccgcggat ccgcgcgcgt tcgtcacctg ccaagactcg aatgaatacg
     3121 cgacgctgag cgggacctgt gaggtctcga aagacgcgcg taggctcgat gaggtctgga
     3181 aagagtccta caaagtctgg ttcgatggga aggacgaccc tcgcctggtt ctgctgcgcg
     3241 tgacgccgga aagcggcgag tattgggata attcgggaag caagggtgtc cgctacgcct
     3301 tccgcgcggt cgccgcgtac gtccaaggca agaagatgga cgagaaggcg gacgacccga
     3361 cgcaacacgc caaagtggcg ctttgagcag gcgcgtcatt gcgccccgag gagattgaac
     3421 atggacgcga aactcatcgt ttacgcggct cttgcggcgc tcggctgctc acaccgaggt
     3481 gatactccag agagcgagag cgcgcctcga acgacgccga ctaccgtcgt cgcgacgcga
     3541 gccaacgagc agtacgtctt cgaatcacca ccggacgccc ccaccaagca gaaacgcttg
     3601 ccaaacgagt cgaccgcgtc gaagcgtgag cgggcacccg aagtgggcga agcgcgcgcg
     3661 aagagcaact tcgtcgagcc tggcacccat ctcgacgata gtgggaatac ggttacgatt
     3721 ccgccggctg acatgaccga cgaggcggcg ccgagcgccg actccagcga ggcgcaaccg
     3781 acggcattcg atcagggcga gagcgagcgc gatcgcacca tcacatcgca gattcggcgt
     3841 gcgctgatgg gagaatcgtc cctctctttc gccgccaaga acgtcgccgt ggtcaccaag
     3901 gatggcaacg tcacgctacg tggaacggtg aagaccgccg cggagcgttc gaccgtagac
     3961 aacttcgcgc gccgcatcgc aggtgagcgc gcggtcgaga accgcatcga agtaacaccg
     4021 taagaggaga caaaatggcg aacaagtccg taatcggaat cgtagaaacg caaatccagg
     4081 cagagtccat cgtgggctcg ctgcaaaacg ctggcttctc ctcgaactcg atctctgcgc
     4141 tgttcccgga tcgccgcggc accgccgact tcgcgcacga gcacaacacc aaggcgcccg
     4201 aaggcgcggt ggccggcgtc gccggcggag gcgtcgtagg cggtacgctg ggcttgctcg
     4261 ccggaatcgg cgcgctcgcc attcccgggc tcggtccgtt catcgctgcc ggtccgctca
     4321 tggcggctct cagcggtgcg gcggctggcg cggcggttgg tggcgtcacg ggggcgctcg
     4381 tcggcctcgg gatcccagag atcgaagcca agaagtacga ggggaagatc cgcggcggca
     4441 acatcatcgt cgccgtacac gtggacgact cgaaagagcg cgagcgcgcg accgagatcc
     4501 ttcgtcgcgg cggcgctcac gacgtaacga gcacgtcgga gtccaacgtt ccgcgcgaag
     4561 agtcgcgcac gaccacgcag cactgaagtg gcacgccacg gactcgagcc gcggatcctt
     4621 cactgggttc gcggctcgga tcttttgtga gacggagcgg ggcggtgtgg cgtgtctaaa
     4681 cagcgacgtg ccgaccatct cgccacaccc gtcgcggctc gatccccgcc ggaaacggcc
     4741 ccatttggac gggcgcgaga tttgcgagat gcagcaccat gccaaactct actccccctc
     4801 ccttcgacgg tcgtaggcgg tctcaacccg cactctccga tgaagcccga gcgaacgagt
     4861 tgctagccga ggaggccagc gtcgagattc ggactctgat tcggtacgag ctcgacttgg
     4921 cgcgagagca gacggcagag gcagtccaaa aggcccacta cgccgcgctg atcggcgctt
     4981 cggcggtcgc cctctccgtc ctcgccttga gctcgatcac ggtcgcactg cttctggctc
     5041 tcggcgcgag cgccgtcccc gcgctggtcg tcgcggcgtt gatgaccgta accggcgcgg
     5101 tcggggcggg ctacatgtac ttcgtgttac cgcactctcc gctcgagtac gcgcgcgccc
     5161 tgctgaagac gcgactcggc gaggttcgtc gcgaaatcga cgcgcacaag gcttctccgc
     5221 agcgcagctg agttgctccg ctgcgcgctc gccttgcgtc agcccggcgg ggccaacgcg
     5281 gctcgaagga acctccgatc ggaatccttc gagccgtgtc ggagtggcgc tacttcaccg
     5341 aactggcagc attgctcggg cgattcgtcg tattcggcgt ggtgccgaac gtagactcgg
     5401 tcgtgcgggc cgcttcgagg tgttgctcga cgcgagtctt gatctcctgc aggtacgcct
     5461 tgagctcggg gctcgaaacg ctcggcaaga gtcgcgtgtt caacgcatcg agcagagcct
     5521 ggtgctcgtc cacctgcgag cgcatgtaag cgcggtcgaa gtccatcccg ttcttgtcct
     5581 tgagggatgt gaggatttgg ttcgcgtcgg tggtcatttg cgcggagagc gggctctcgg
     5641 ccgacgtgat gttcagcttc gcctgcttct tctgcgcgtc gccgtggtga gtgatcatca
     5701 tggacgcgaa ctgcaagacc ttttggtttt tggacttgct gcgcgcaagc ttcgcttgct
     5761 cgatttctgc gctgttggca ccgtcggtga tcccggcgat ttgagcgtcg ctcagcgcgc
     5821 tcgggggcgc gtcgtcggtc ggcttgttcg agtttgccga cgtcgagtcg gacgtcgtgt
     5881 ccttgcgctg ctcggtggcc gtcgtcgcga ctgcgtcggg cgccggaccc gtgggcgggt
     5941 tggtgtcgtt ccccatcgcc cccggagtcg acgctgtctg catgttcgcg gcgtcggcgt
     6001 tcttggcggg ttcatgctcc gcgcattgca acatcatgag cgcgaggctg cctgccagcg
     6061 tcgctagggt tctttgcctt accattctgg tctccttcta tcgccgtctc ccgacggcta
     6121 cgcgcgcctc ggcgcgcaat gtgtcattcg gtcgagtgac ccgctaaatc ccgagcgagc
     6181 gttggtttca gcgggccacc ttggcccaac gagggatctc tcagcactca gagcgtgtac
     6241 aagaactcgg tt
//
LOCUS       DASTKS010000907         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1487282, whole genome shotgun sequence.
ACCESSION   DASTKS010000907 DASTKS010000000
VERSION     DASTKS010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487282"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFQ35_18950"
     CDS             complement(<1..415)
                     /locus_tag="VFQ35_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932793.1"
                     /translation="MSARTFANLSLIVSISAACSVERHSGEAAGGSAGGRSTSGGKSG
                     VNSGGHGGAGGAPSGGRAGESAQGGAPAAGVGGLETGGQPSDGGTSQGGMSGGGAVSN
                     GGSTGAGGDGAGGTTTPVGANSGGTQPNTGGTSPSS"
     gene            908..1243
                     /locus_tag="VFQ35_18955"
     CDS             908..1243
                     /locus_tag="VFQ35_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932794.1"
                     /translation="MTRSTLRVGLLAIIASFSLTSVLACSADGDGPGTGGTAGAPVTS
                     GGGGGTGGSSGGTGGGGTGGIAPCPSGFSPPQGEPCEPGDGFVCSGYIYPFVTVDCNC
                     ISGKFSCAL"
     gene            complement(1272..1700)
                     /locus_tag="VFQ35_18960"
     CDS             complement(1272..1700)
                     /locus_tag="VFQ35_18960"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9932795.1"
                     /translation="MTAPGEPIRLFDYGSLMAGERDHALLASAKLIGPARTRPAFHLV
                     DLGVYAALVAGGDVSVVGELYLVERKACFAIDVHKQCSVLFDRVAIELDDGSKAHAYL
                     MPEERVRGKRRIKCGSWRDRFAPPPRERYRSAFVEALRKR"
     gene            complement(1746..2678)
                     /locus_tag="VFQ35_18965"
     CDS             complement(1746..2678)
                     /locus_tag="VFQ35_18965"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9932796.1"
                     /translation="MADILELEVFHCVVKHGNYAKAAEELGLSPSGVSRLVSRLEEKL
                     GARLLQRTTRKLALTEAGSTFHGRTAQVLLDLAEAEAEVREQGLRPRGTLRATASVVF
                     GQLHVAPLLEKLLQLYPELSIDLSLADRFVDLIDEGFDLAIRIGALSDSQLVARRLCT
                     NHRILVASPSYVSRRGVPAQPSDLAAHDCLAFNQFARPREWRLQGPDGALVTTNITAR
                     FASNNPIALTEAAKRGLGITVGATLSIAPALLSGELVRVLPEFEFETSAMFAVYPSAR
                     QLSTKVRATVDFLAQHMRDPTDWDQSLQGKVPGF"
     gene            2854..3711
                     /locus_tag="VFQ35_18970"
     CDS             2854..3711
                     /locus_tag="VFQ35_18970"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9932797.1"
                     /translation="MSRSSDAWFRYSSEYEFFVPISWSGAVWHRRRETVVGPGFVVCA
                     QPGEVLACKRVLAAGSACSLVFDADTLRRHLRERGVELEGVRFRPLSRLSAELRRRLG
                     ALLALLDTGESPFELEATLAEFVARMVDELLDESPQPLQSRSFAAKMAEQIRSYLVED
                     PCVTIDLDTLARQTGLSRFQVVRIFKRRYGLPPHSYQLQRRLGFAQRRLREGARPAQV
                     AIEFGFVDQSHLTRHFKRVFGVTPAQYARASSEAVADISVELLSARALEDRRDTRLAV
                     ASGLSFTRH"
     gene            3778..4269
                     /locus_tag="VFQ35_18975"
     CDS             3778..4269
                     /locus_tag="VFQ35_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932798.1"
                     /translation="MRESFMALSVVIASTFSSPAHADPSGATASTPRFFQPLELELPF
                     AKPLSFSFSSEPVPGFETLRLGTYRAEAVWWRAGDLSLRTYGQVAPALELDCSSTCQP
                     MLERVVGLDGRFDLGSAGRALPAAYVYVRGQSSQVVPTVPGTRSPIKSNLLGFGLGGL
                     LDF"
BASE COUNT          702 a         1377 c         1406 g          789 t
ORIGIN      
        1 cgctgcttgg agacgtgccg ccggtgtttg gctgagtccc accgctgttc gctccgacgg
       61 gtgtcgtcgt gccgccagca ccgtccccgc cggcgccggt ggaaccgccg ttcgaaacgg
      121 caccgccgcc cgacatgcct ccctgagacg tcccgccgtc cgagggttga ccacccgttt
      181 ccaaaccacc gacgcccgcc gcaggcgctc cgccttgggc ggattcacca gcgcggccgc
      241 cgctcggcgc gccgcctgca cccccgtgtc ccccggaatt gaccccgctc ttgccgccgg
      301 atgtcgaacg accgccggcg ctgccgccag ctgcttcgcc gctgtgccgc tccacactac
      361 acgcggccga tatcgatacg atcaggctca aattagcaaa agttctcgcg ctcacttgga
      421 gccctcccaa cctgcttgtc gcttggagaa atagcgtgca ctcggagcga cacccgaatc
      481 gctgttcgga aatgcgcgcg cctccaaaac gtgggcgcaa gcctatcaca cgaacgcctg
      541 gctcgtgggc gagcggcgcg ctgcgcccct aaaataagcc gtgcttctcg gctggcgggc
      601 cagctctgtt cgcccggtct tggaagcacc gcgcgactcg tcgctccgtc gcaccgtgtg
      661 cggacggcga gcagtttcga cgagactact cttgcgtgcc cgagcggcgg ctcttcgaac
      721 aacgttggac gagagagccg cagcagcaac cgcgacgacc ggactctttg agtcgccctg
      781 agccactgtg tgccgacaga cctctgcgcg cgcagctcat gcattgaaat cagcctctct
      841 tgcgccaacg cgagctcgcc tccctacggc acgcacgttg ctacgacagc ggtgggagga
      901 acttcaaatg actcgctcga cactcagggt tggattgctc gcaatcatcg caagcttctc
      961 gctgactagc gtgctcgcct gcagcgccga cggcgacggt cctggcacgg gggggacagc
     1021 gggcgcgccg gtgacgtctg gcggcggagg aggaacggga ggtagctcgg gtggcaccgg
     1081 tggaggtgga accggtggca tcgcgccttg tccttcgggg tttagccctc cacaaggcga
     1141 gccatgcgag ccgggcgacg gcttcgtctg ctccggttac atctacccat tcgtcaccgt
     1201 cgactgcaac tgcatctccg gcaagttctc ctgcgccctg tgaggctcgc gagcttttct
     1261 gtgcggtgct gtcagcgctt gcggagtgct tcgacgaagg cggagcggta gcgttcacgc
     1321 ggcggcggcg cgaaccgatc gcgccagctg ccgcacttta tgcgccgttt ccctcgcact
     1381 cgctcttccg gcatgaggta tgcgtgagcc ttgctgccgt cgtcgagctc gatcgcgact
     1441 cggtcgaaca acaccgaaca ttgcttgtgg acatcgatgg caaagcaggc ctttcgctcc
     1501 acgagataga gctcacccac gacgctcacg tcgccgcctg cgacgagcgc cgcgtacacg
     1561 ccgagatcaa cgaggtgaaa cgcgggtcga gttcgagccg gaccgatgag cttcgccgat
     1621 gcgagcaatg cgtgatcgcg ctcgccagcc atcaaagagc cgtagtcgaa tagccgaatc
     1681 ggctcgcctg gggcggtcat tggcgccagt ctgccaaaaa ccggggctac cgagaacggt
     1741 ctagatcaaa agcccgggac cttgccttgg agcgactggt cccaatcggt gggatcgcgc
     1801 atgtgctggg ccagaaaatc taccgtcgcg cgcaccttgg tggatagttg gcgcgcggac
     1861 ggataaacgg cgaacatggc cgaggtctcg aactcgaact cgggcagtac gcgcacgagc
     1921 tcgccactca gcagcgccgg cgcgatcgag agcgtcgcgc cgaccgtgat cccgagaccg
     1981 cgcttggcag cttcggtgag cgcgatgggg ttgttggacg cgaaccgcgc cgtgatgttg
     2041 gtcgtcacga gggctccgtc gggcccttgc aagcgccact cgcgcggtcg cgcgaattga
     2101 ttgaaggcca ggcagtcgtg ggcggcgaga tcgctcggct gggcgggcac gccgcgtcgc
     2161 gaaacgtagc tcggcgacgc caccaagatg cggtgattgg tgcacaatcg gcgcgcgacg
     2221 agctgagagt cggagagggc gccgatgcgg atcgccaagt cgaagccttc gtcgatgaga
     2281 tcgacgaagc gatctgcgag cgacagatca atcgagagct cggggtagag ctgcagcagc
     2341 ttctcgagca gcggagcaac gtggagttga ccgaaaacta cggatgcggt cgcgcgcaac
     2401 gtgccacgag ggcgcaatcc ctgctcgcgc acctccgcct ctgcctccgc caaatcgagc
     2461 agaacttggg ccgtgcgtcc gtggaaggtc gagcccgcct cggtcagcgc cagcttgcgc
     2521 gtggttcgct gcagcagtct cgcgcctagc ttctcttcga gcctcgatac caggcggctc
     2581 acgccagatg ggctcaaccc gagttcttcc gccgccttcg cgtagtttcc atgcttgacc
     2641 acacagtgga aaacctcgag ctccaggatg tccgccatgc gcgctcagtc ggccagattc
     2701 gtgacgccgc gtcacgactg aagtgtcggc cctccgattt ttcacagcat tcgagtgtga
     2761 gacagtgcgc gagtcatgtc gataacgaat gaaacgggag agagggtcac gttccgtcgt
     2821 cgtcgcgagc tgccaggcat tgagctccgg acgatgagcc gatcgagcga tgcgtggttt
     2881 cgctacagca gcgagtacga gtttttcgtg ccgattagct ggagtggcgc ggtctggcat
     2941 cgacgacgcg agacggtggt ggggccgggc ttcgtcgtgt gcgcgcagcc cggagaggtg
     3001 ctcgcgtgca agcgcgtact agccgcgggc agcgcgtgtt cgttagtgtt cgatgcggac
     3061 acgctgcgtc gtcatttgcg cgaacgtggt gtcgagttgg agggagttcg gtttcgaccg
     3121 ctctcacgcc tttcggcgga gcttcggcgg cgcctgggcg ccttactcgc gctgttggac
     3181 acgggtgaat caccgttcga gctcgaggcg actctggccg agttcgtggc ccgtatggtc
     3241 gacgagttgc tcgacgagtc gcctcagcca ctgcagagcc gcagctttgc cgcgaagatg
     3301 gcggagcaga ttcggagcta cctcgtggaa gatccgtgcg tcacgatcga tctcgatacg
     3361 ctcgcgcgcc aaacgggtct cagccggttt caggtcgtga gaattttcaa gcgccgttac
     3421 ggtttgcccc cccactcgta ccagctccag cggcggcttg gcttcgcaca acgtcgcttg
     3481 cgcgagggcg cgcgtccggc gcaggttgcc atcgagttcg gtttcgtcga ccagagccac
     3541 ctgacccggc acttcaagcg cgtgttcggg gtcacgccgg cgcaatacgc gcgcgcctcc
     3601 tccgaggcgg tagccgacat ctcggtcgag ttgctctcgg cgagggcgct cgaagaccgg
     3661 cgggacacga ggcttgccgt ggcatcgggt ctcagcttca ctcggcactg agggcgcact
     3721 gagggcgcac tgagggccct gacccgctcg catgagcccg gtgctacact ggcgctcatg
     3781 cgagagtcgt tcatggcgct atcggtcgtc attgcgtcga cgttctcgtc gccagctcac
     3841 gccgacccga gcggcgcgac ggcgtcgacg ccgcggttct ttcagccgct cgagctcgaa
     3901 ctgccgtttg cgaagccgct cagcttttcg ttttcgagcg agcccgtccc cgggttcgag
     3961 acgttgcggc tcggcactta ccgcgccgag gcggtctggt ggcgcgcggg cgatttgagc
     4021 ttgcgaacgt acgggcaggt tgctcccgcc ctggagctcg actgctcgag tacctgccaa
     4081 cccatgctcg agcgcgtcgt gggtctcgat gggcggttcg atttgggtag tgcaggtcgc
     4141 gcccttccag cggcctacgt ctacgtgcgg ggacaatcgt cgcaggtggt tccgacggtt
     4201 ccaggaacga gaagcccgat caagtcgaac ttgctcgggt tcggcttggg ggggcttctc
     4261 gacttctgac gcgc
//
LOCUS       DASTKS010000908         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_592811, whole genome shotgun sequence.
ACCESSION   DASTKS010000908 DASTKS010000000
VERSION     DASTKS010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592811"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VFQ35_18980"
     CDS             <1..1704
                     /locus_tag="VFQ35_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932799.1"
                     /translation="RAFDSPLPSTGPMRHGRAAAVPEPREPIPTLADEDLPSSTDTLR
                     PAGAVPPPPRGVPRPGGFGAGGALAERASVTPIPGIRVAARSSSLPGARPSSLPATAS
                     AAAPAAAPALTPADFRVTPRDPIELSGMLPRSFPAPVEARSENTPVPAMPAAAPLPSV
                     VAGRPSGSPLSSSLGAASSSLIPGGEGLPLGLRVAIGFLSVLCVLLAVALVFALRALG
                     TPPAAAATQAVSPAPASSTVAPAPLVEGCVLRAPAAKLASSVERGIEPRFLDLGADRV
                     AVGFAATRSQAAGIVVDLAQLDATAVFDEARERPVLGVAPVSAEPLAFNADRDDGALT
                     MPHSLDSTPRSVLGYTNEGLAKSVAGGAPELVWPMTHRVATEPRVAHAGSDAYAVALR
                     QGGLGGNVLSGWLSAELKPMSELSPVAAGAKWVGSPAVAARDGAMVVAFAARDSESEA
                     WRIRLGRAEARRAVESVLVHATPKEGLGNGAIAPTLAPFGKDRWVLQWTQGSAGQYRV
                     YVQELGSDLQPLGTPVAASPKGASAGLGAVRVRGESALSVFVLTVGGRDELWGASVTC
                     H"
     gene            1695..2561
                     /locus_tag="VFQ35_18985"
     CDS             1695..2561
                     /locus_tag="VFQ35_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932800.1"
                     /translation="MSLSRVAVTFAAALTIAGVPACSFIFNSGPSEVAQGRYYSSGNA
                     EYDAFFVDVYQQQLVMARAPGELTDARAALTQALVLDASAQNAELAEKLRLALNSLGQ
                     RGARVHFEVRLPSPPDPEHSIALISASPPPEGNERTSLAAAEAALTRLLRLAATMHQS
                     EAELAKLRTSVPALEAKVDAAFQERGPRQRERTRQNLKDAERVMVLMQARADETASPV
                     DGLYRELNRVFASSEPPPSEGASPPKPEEKPRPRPAPRPRPDVAQPAPRSEGAPVASP
                     KPTAPKSADFEP"
     gene            2941..3879
                     /gene="gspC"
                     /locus_tag="VFQ35_18990"
     CDS             2941..3879
                     /gene="gspC"
                     /locus_tag="VFQ35_18990"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET9932801.1"
                     /translation="MNYQAEAWLGRAVSLCVFALIGIAAFLQARALSLLIASSRLLSP
                     LGPRERARAGEPELSPGRNAGMQSSKSPGLERESLLPDEVVAPVDWTDPLRAPSCEGL
                     TAFIVTESADRLWSVATLGVAGEPRAQMRRVGDHVSGKQVEFIGFNARAGSPAVWLSS
                     SRGGLCQALLQYPPSHAPTTAAKAMARKASESTLTREVAAKIARISETEFNIERSAIQ
                     QILENQSELLRFVRVAPELEDGRAVGLRLVELGRKSVLSSLGLRAGDRIDSINGFNIA
                     TPEKALEAFAVLRVASNLDVRLQRGNATLNVAYRVR"
     gene            complement(3890..>4253)
                     /locus_tag="VFQ35_18995"
     CDS             complement(3890..>4253)
                     /locus_tag="VFQ35_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932802.1"
                     /translation="GGAGTDGPTRVYSGGAADYDSDELTVQVQSIRRGLPVPLLSRDE
                     IMLPLTMARKAPEEPKPQLAETRPAERRRLIIARRRTLRRMQWVIAGVLLLAALTVTI
                     RYRPAWLGRVAAKFHSSR"
BASE COUNT          647 a         1447 c         1427 g          732 t
ORIGIN      
        1 cgcgcgttcg actcaccgct gccgagcacg ggccccatgc gtcacgggcg cgccgctgcc
       61 gtccccgagc cccgtgaacc gatcccgacg ctcgcggacg aagatctccc gagctcgacc
      121 gacacgttgc gcccagccgg agccgttccg ccaccgccgc gcggcgttcc tcggcctggc
      181 ggtttcggcg ccggtggcgc gctcgccgaa cgcgcatcgg tgacacccat tccaggaatt
      241 cgggtcgcgg cgcgttcgag ctcgctgccg ggagctcggc cgagttcgtt gccagcaacc
      301 gcgagcgccg ccgcgccagc ggccgccccc gcgctcacgc ccgcggactt ccgcgttacg
      361 ccgcgcgatc caatcgaact ctcgggcatg ctgccgcgaa gtttcccggc tcccgtcgaa
      421 gcccgttcag agaatacgcc ggtgccggcg atgccagcgg cggcgccgtt gccgtccgta
      481 gttgcgggga ggccgtcagg ttcgccgctc tcgagctctc tcggcgcggc cagcagctcg
      541 ctcattccgg gtggcgaagg cctgcctctc ggactgcgcg tggcgattgg gttcctatcg
      601 gtgctttgtg tgctgctcgc agtggccttg gtgttcgcgt tgcgagccct cgggacccct
      661 cccgcggccg ccgcgaccca ggccgtatcg cccgcgccgg cgtcgagcac ggtggcaccc
      721 gcgccattgg tcgaaggctg cgttttgcgc gcgcccgcag caaagctcgc gtccagcgtc
      781 gagcgaggca tcgaaccacg ctttctggat ttgggtgcag accgtgtggc cgtcggcttc
      841 gcggcgacgc gctcgcaggc ggccggcatc gtcgtggacc tcgctcagct cgacgcgact
      901 gcggttttcg acgaagcccg cgagcgcccg gtcttgggtg tcgctccagt cagcgccgag
      961 ccgctcgcgt tcaacgccga ccgcgatgac ggcgcgctca cgatgccgca ctcactcgac
     1021 tctacaccgc gcagcgttct cggttatacg aacgaaggtc tcgcgaagag cgtcgccggt
     1081 ggcgcgccgg agctcgtttg gccgatgacg caccgggttg ccaccgagcc gcgtgtggcg
     1141 cacgccggaa gtgatgcgta tgcggtcgcg cttcgtcagg gtggcctcgg tggcaacgtg
     1201 ttgagcgggt ggttgagcgc cgagctcaag cccatgagtg aactttcgcc ggtcgcggcc
     1261 ggcgcgaagt gggtcggttc accggcggtt gcggcgcggg acggagccat ggtggtggcg
     1321 tttgcggctc gcgacagcga aagcgaagcc tggcgcatcc ggctcggtcg agccgaagcg
     1381 cggcgtgcag tcgagagcgt tctcgtgcac gctacaccga aagaagggct cggaaacggc
     1441 gcgattgcgc cgacgctcgc tccgttcggc aaggatcgct gggtgctgca atggacccag
     1501 ggcagcgcgg gtcagtatcg cgtctatgtc caggagctcg gctccgacct gcagccgctg
     1561 ggcacgcccg tcgcggcatc acccaaaggc gcgagcgcgg gtctcggtgc cgtgcgcgtt
     1621 cgcggcgaga gcgctctgtc cgtcttcgtt ttgaccgtcg gcgggcgcga tgaactctgg
     1681 ggagcgagtg tgacatgtca ctgagccgcg tcgccgtgac gttcgccgcc gcgctgacga
     1741 ttgccggcgt gccagcgtgc tcgttcattt tcaactcggg cccgtccgag gtcgcgcaag
     1801 gtcgctatta ttcctctgga aacgcagagt acgacgcgtt cttcgtggac gtttaccagc
     1861 aacagctcgt gatggcgcgc gcgccgggcg agctcaccga cgcgcgcgcg gcgctcactc
     1921 aggcgctcgt gctggatgca agcgcccaaa acgcggagct cgccgagaaa ttgcggttgg
     1981 cgctgaactc gctgggacaa cgaggagcgc gagtgcactt cgaggtgcgc ttgccgtcac
     2041 cgccggatcc ggagcacagc atcgcgctca tatcggcgtc tcctccgccc gagggcaacg
     2101 agcgcacgtc gctcgccgcc gccgaggctg cgctcacgag gctcttgcgg ctcgccgcga
     2161 cgatgcacca gagcgaggcc gagctcgcga agctccgcac ttctgttccc gccctcgaag
     2221 ccaaggtcga cgctgcgttt caagagcgtg ggccgcgcca gcgcgagcgc acgcggcaaa
     2281 acctcaaaga tgccgaacgc gtcatggtgc tcatgcaggc gcgcgccgac gagactgcca
     2341 gccccgtgga cggtctctat cgagagttga accgcgtctt cgcgagctcg gaacctccgc
     2401 cgtcggaagg cgcttcgccg cccaagcccg aagagaagcc ccgtccccga cccgcgccac
     2461 ggccgcggcc agacgttgct cagcccgctc cgcgcagcga gggagctcct gtcgcgtcgc
     2521 ccaagccgac ggctcccaag tccgcggatt tcgagccctg atcttcggcg ccgacttcgt
     2581 agagcgctcc cctaacagag acacgagctc gacgcccgag tgacgctgtt actgcgttac
     2641 tcgacacgcg agatcggatg ccactctgtc aagagcgcct gaactcgact gcgtctcgtc
     2701 gccatcgtgg cgctcgtcta ctgccctaat cggagtgcag cgccgatttt gctccagtag
     2761 cgcttcggac gcgtgctggc acatggcttg cgtaggggct cgcatgcgtc tcgaagtccc
     2821 gctcttcatc ggcgccgtat cgaagtctcg gcatcatacg ttgcgccgtc tcggatccat
     2881 ctctagcgcc gggcagttgg tgagtgagct cgctccgaca gttgcccgtt gcgaggcagg
     2941 atgaactacc aagccgaagc atggctcggc cgtgcagttt ctctctgcgt cttcgcgttg
     3001 atcgggatag ctgcgtttct gcaggcgcgc gctctctcgc tcctcatcgc ttcctcgcgt
     3061 cttttatcgc cgctcggtcc ccgcgagcgg gctagagccg gcgagccaga gctgagtccg
     3121 gggaggaacg cggggatgca gtcttcgaaa agccccgggc tcgagaggga gagtctgctc
     3181 ccggacgagg tcgtagcacc cgtcgattgg acggacccgt tacgcgcgcc gagctgtgag
     3241 gggctcactg cgttcatcgt gaccgagtcg gcggatcggc tatggtcggt cgcgacgctc
     3301 ggggtagcgg gggaaccgcg agcccagatg cgacgtgtcg gcgatcacgt ctcgggtaag
     3361 caggtcgagt tcatcgggtt caatgcgcgc gccggttcgc cggcggtttg gctttcgagt
     3421 tcacgtggcg ggctgtgtca ggcgctgctt cagtacccgc cgtcgcacgc cccgaccacc
     3481 gccgcgaaag ccatggcacg caaagcttcc gaatcgacgc tgactcgtga agtcgccgcc
     3541 aaaatagccc gcatcagcga gaccgagttc aacatcgagc gcagcgcgat ccagcaaatc
     3601 ctggagaacc agtcagagct cttgcgcttc gtgcgcgtcg caccggagct cgaggacgga
     3661 cgcgccgtcg gtctccggct ggtcgagttg ggccgcaaga gcgtgctctc ctcgctcggc
     3721 ctacgagcag gcgaccgcat cgactcgata aacggcttca acatcgcgac gcccgaaaaa
     3781 gctctcgaag cgtttgcggt gctgcgcgtc gcatcgaacc tcgacgtcag actccaacga
     3841 ggaaacgcga cgctgaacgt cgcttaccgc gttagatgat gcgtggcggc tagcgggagg
     3901 aatgaaactt cgccgcgacg cggccgagcc acgccggtcg ataacggatc gtcaccgtca
     3961 gcgcggcgag caacaacact cccgcgatca cccattgcat acggcgcagc gtgcggcggc
     4021 gcgcgatgat caaccttcgg cgctccgcgg gacgagtctc tgcgagctgg ggctttggct
     4081 cctcgggcgc ttttcgcgcc atggtgagcg gcaacatgat ctcgtcgcgc gagagcagcg
     4141 gaaccggcag cccgcgtcga atgctctgca cctggaccgt gagctcgtcg ctgtcgtagt
     4201 cggcggctcc gccagagtag actcgtgtcg ggccgtccgt gccggctccg ccg
//
LOCUS       DASTKS010000909         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1487494, whole genome shotgun sequence.
ACCESSION   DASTKS010000909 DASTKS010000000
VERSION     DASTKS010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487494"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFQ35_19000"
     CDS             complement(<1..231)
                     /locus_tag="VFQ35_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932803.1"
                     /translation="MGRHDDPAPGDYVLTVELNLDAIPPGTSGPGWSALAHGNFRLTR
                     DGTELASEDVESRSRAEFAYGRPLGVGASEVVD"
     gene            654..893
                     /locus_tag="VFQ35_19005"
     CDS             654..893
                     /locus_tag="VFQ35_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932804.1"
                     /translation="MLTDVTTLRHIHRYMNDRRRVLLADGRIAKIVRVDTVFPANDTT
                     VTVWTDSGGSGPGVSKVRLDDVVGLAAETLAPPAR"
     gene            complement(996..2444)
                     /gene="dnaA"
                     /locus_tag="VFQ35_19010"
     CDS             complement(996..2444)
                     /gene="dnaA"
                     /locus_tag="VFQ35_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9932805.1"
                     /translation="MSASSLSEHAERSWKEAVDETRRRSPASFEQWFSSVQFDGLDSG
                     VLSLAARDEWVRDWVVAHFLPDLLANLERKLGEEANAGVRVRWRIEPQITLPVCEGRS
                     VAVSAKPRASEAPPPSSAVPRRSDRAPTSVTYSRATLAAALNQKHTFANFVVGPSNAL
                     AYAAAFASAGGGGPRYNPLFIAGGTGLGKTHLMHAIAHKVLEEKPSARIVYVSAERFT
                     NDFIEALQHHRMDEFRTRYRAECDLLLVDDIQFLAGREQTQEEFFHTFNALRDADHPI
                     VVTSDKYPQQLQRMPERLVSRFTSGLVADIQVPQLETRVAIVKKKAQIEGIDLSDEVA
                     ELLAQNVQSNVRELEGALIRLAAKSSLTGRAIDEEFAESELAMVSPRRPDMVSVEDIQ
                     RAVCSHFRLSNSELLSKDRHKSVAFARQVAMYLCRQRLKCSFPELGRAFGNRDHTTVM
                     SAVRRVEALRQRDPQVNAHLEAIEQKLAASEE"
     gene            complement(2686..>3103)
                     /locus_tag="VFQ35_19015"
     CDS             complement(2686..>3103)
                     /locus_tag="VFQ35_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932806.1"
                     /translation="RVTRESTTRVFVERANQMGRSVSMNIASGLLTPQITEVDAERNR
                     AQLEREQISFDFAKLLTVDEARTREARNPCSLRVDDPASFERPLRVGNLLFELWTRAM
                     LAAPSLRRTHRRKLGSTARPSKARKRGSMTSFETAG"
BASE COUNT          539 a          965 c         1039 g          560 t
ORIGIN      
        1 gtctacgact tcgctggccc cgacgccgag cgggcggccg taggcgaact cggctcgcga
       61 acgcgactcg acgtcctcgc tcgcgagctc cgtgccgtct cgagtgagcc gaaagttgcc
      121 atgggcgagc gccgaccagc cgggcccgct ggtgcccggg gggatcgcgt cgaggttgag
      181 ctcgacggtg agcacgtagt ccccgggggc ggggtcgtcg tgtctgccca ctgggattac
      241 cctcgggaaa ctcggccaaa gtccgcgctc gagggccgtc gcgagggcgt cgtccagctc
      301 agcgaagcag agttttcgcg actccggcgg ttccacacac tccgacgggc tcactcccgg
      361 gacgaggttg acccacaggg gagccgagtg tcgaaccatg ggctccggcg gaggcggtag
      421 ggccgcgccc tgcacgaccg cggtgtgcga cgaaaaggag gaaacgcagg aagggagcac
      481 ggaaagcgcg aaaatcgcgg caaaacgcgg tgaaggcatg acggcggaac gatagcgaat
      541 aggactccaa tcacgccaac ttccggcagg cgccggcggt cagcggcgct acccggcgga
      601 tctggcggcg gggggcgaaa ggcgatcttt gggcagattt tgaaagctgc gttatgctca
      661 cagacgtgac gacgctccgg cacatccatc gctacatgaa cgatcgtcgg cgtgtcctgc
      721 tcgccgacgg acgcatcgcc aagatcgtgc gcgtcgatac ggtgttcccg gcgaacgata
      781 cgacggtcac ggtttggacc gactcgggcg gttctggtcc cggcgtttcg aaggttcgcc
      841 tcgacgacgt cgtcgggctc gccgctgaga cgctcgctcc gcccgcacgc tgagccgcgc
      901 cgattcggtc gaacccacgc attgcgggtt cgcgcgcact gaatgggtct tcggcggcct
      961 cgccttcagc gaagccacca ttcacgcgcg gtcgctcact cttcggacgc agcgagcttc
     1021 tgctcgatgg cttcgaggtg cgcgttcacc tgcgggtcgc gctgacggag cgcttcgact
     1081 cgacgcacgg cgctcatcac ggtcgtgtga tcgcggttgc cgaaggcgcg cccgagctcg
     1141 gggaagctgc acttgaggcg ttggcggcag aggtacatcg cgacctgccg cgcgaaggcg
     1201 acgctcttgt ggcgatcttt gctcaagagc tccgagttcg acaagcggaa gtggctgcac
     1261 acggcgcgct ggatgtcctc cacgctgacc atgtccggtc ggcgcggcga gaccatggca
     1321 agctcgctct cggcgaactc ttcgtcgatg gcgcgaccgg tgagggaaga cttggcagcc
     1381 agacgaatca gcgcgccttc gagctcgcgg acgttgctct gcacgttctg agcgaggagc
     1441 tccgcgacct cgtcggagag atcgatgcct tcgatctggg ccttcttctt gacgatggcg
     1501 acgcgcgtct cgagctgcgg gacctgaatg tcggcgacca agcccgacgt gaagcgcgag
     1561 acgagccgct ccggcatgcg ctgaagctgc tggggatact tgtcgctcgt gacgacgatt
     1621 gggtggtccg cgtcgcgcag ggcgttgaac gtgtggaaga actcttcctg cgtctgctcg
     1681 cggccggcca agaactggat gtcgtccacg aggaggaggt cgcactcggc gcgatagcgg
     1741 gtgcggaact cgtccatgcg gtggtgctgc agcgcctcga tgaagtcgtt ggtgaagcgc
     1801 tccgccgaga cgtagacgat gcgcgcgctc ggcttctctt cgagaacctt gtgcgcgatc
     1861 gcgtgcatga ggtgggtctt gccgaggccg gtgccgccag cgatgaagag ggggttgtag
     1921 cgaggccccc cgccgcccgc cgacgcgaac gcggctgcgt acgctagggc gttggacgga
     1981 ccgacgacga agttcgcgaa ggtgtgcttc tggttgagcg cagccgccag cgtcgcgcgg
     2041 gagtaggtca cgctcgtggg cgctcgatcg cttcgacgag gcactgccga cgaaggcggc
     2101 ggcgcttcgc tcgcgcgggg cttcgccgag acggccacgc tgcgaccctc gcagacgggc
     2161 agcgtgatct gcggctcgat gcgccaacgc acgcgcaccc ctgcgttcgc ctcctcgccc
     2221 agcttgcgct ctaggttcgc gagcaggtcg ggaagaaaat gagcgacgac ccaatcccga
     2281 acccactcat cacgagccgc gagcgagaga acgccactat ccagtccatc gaactgaaca
     2341 cttgagaacc actgctcgaa cgacgcgggc gatcgccgac gcgtctcgtc cacggcttct
     2401 ttccaggacc gctcggcatg ctcactgagg gaggatgcgc tcactttgga acctgaggtg
     2461 gggatctcga aagtattgct gatcacggag gtctcgcggg aggaattccc cattttgtcc
     2521 acaggcctgt ggactagctg ggcgggtcaa gcgttacgct ccatttcggg agcgctcgat
     2581 tgggggtcgg tttgaagaac aaaccgtccc aggttggtca acttcggagc accgaagctt
     2641 tgttctctcg gaccgtgttc gcgccatctt ccgatagcgc tcgactcaac ccgctgtttc
     2701 aaagctagtc atcgaccctc gcttgcgcgc cttcgacggc cgggcggtcg aaccgagttt
     2761 tcgtcggtgc gtccgccgca gcgaaggggc agctagcatc gccctcgtcc aaagttcaaa
     2821 caaaagattt ccgacacgca gcggccgctc aaatgacgcg ggatcgtcga cgcgcaacga
     2881 acaagggttc ctagcttctc gagtgcgcgc ttcgtcgacg gtcagtaact tcgcaaagtc
     2941 gaaagagatt tgttctcgct cgagctgtgc gcgatttcgt tccgcatcaa cttccgtgat
     3001 ttgcggcgtc aagagtcccg acgcgatgtt catgctgacc gaacggccca tctggtttgc
     3061 tctttcaaca aacactctcg tggttgattc tcgcgtgacg cga
//
LOCUS       DASTKS010000910         9654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1709925, whole genome shotgun sequence.
ACCESSION   DASTKS010000910 DASTKS010000000
VERSION     DASTKS010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709925"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            675..1949
                     /locus_tag="VFQ35_19020"
     CDS             675..1949
                     /locus_tag="VFQ35_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932807.1"
                     /translation="MARSRGTEPAGALPAKRSGAGRARDHRPSQDTGTPLERQRLTWK
                     ELVQPPYSKLDDDAFTRVRVILMNGIEAGALRFQHLCGRMNRELQLDLARIRRIEQHQ
                     QTMVNWLNPPDQNVLETTIGFEQVAIEVTAAIALAEPDPYLAQVYRYGLLEDFDHMYR
                     YSALMDRVEGKDANTLLQSYTDILPGRPTAVEHRAPEDELRACYDKRTATLLTKLHAN
                     TLISGEFQTHDYYMTVGPTYSDPLGRQLYAEIASIEEQHVTQYESIVDATETWLEKWL
                     LNEATEVYNYYSCVESEKNSRIKAIWERFLDYELGQLAHVRTLFERVERRDAAEVLGT
                     KIPGPIAYESHREFVREVLRREANLRSVGSAFVPASEEPQGGATAIYRAHMNSAGSPS
                     ESVATGYSWSPGTELSRKQPRTGNGVEVTQPL"
     gene            1993..2721
                     /locus_tag="VFQ35_19025"
     CDS             1993..2721
                     /locus_tag="VFQ35_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9932808.1"
                     /translation="MSTQAELGFNRTGIATSPKLSAEMVQGTTEFTSTFDGDGASIAA
                     VREEYARSGEKVGSVPPPLTLGGVASAAVQGIRGTHPVEFLDKIGARLGFERTGVRLY
                     EALISKFESSGGFTGGPTRRELEEIMHEEYLHFALLVDTITKLGGDPTVVTPSADVAA
                     TLSRGILDVLVDPRTTFVQSLEAILAAELVDNDAWEMLILGARQRGDDELTARFESAR
                     ADEDKHLARVRAWLTAAQGRPVGA"
     gene            2743..3642
                     /locus_tag="VFQ35_19030"
     CDS             2743..3642
                     /locus_tag="VFQ35_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9932809.1"
                     /translation="MRVHHLNCVSTCPLGGWFMDHRTRDVLARGRLVCHCLLVEVGGE
                     LVLIDTGFGLLDVANPQSRLSAFFLSLVRPEFREEMTAVRQIAKLGLDPKDVRHIVMT
                     HLDFDHAGGLDDFPGARVHLLAREHEHATARRTWLDKQRFRPQQWSTQASWQLYEPAS
                     GESWFGFARAQPLSGLPPEILLIPLYGHTLGHAGVAVAQGDHWLLDAGDAYFFQGEMN
                     LERPWCTPGLRFYQTMMEKDREARLTNQERLRELRRSESQRVEVFCSHDVSEFERLSG
                     HSAALPAEYLGQTGAPTSFAPAE"
     gene            3681..3866
                     /locus_tag="VFQ35_19035"
     CDS             3681..3866
                     /locus_tag="VFQ35_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9932810.1"
                     /translation="MVDLNGAVVVVTGASSGIGRAAAIEFARRGARLVLAARRSQALE
                     GTARICEREGGSPVRGR"
     gene            3871..5331
                     /locus_tag="VFQ35_19040"
     CDS             3871..5331
                     /locus_tag="VFQ35_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9932811.1"
                     /translation="MRDCEAVIVGAGPNGLAAAIVLAAAGVKPLVLERAQQPGGGART
                     SELTLPGFFHDTCSTIHPLAFGSPLFRRLRLERYGLAAVHSPAALAHIVDTNTVVTLE
                     RSLGAMVGKLGGDGTAYHDVFAPIAEHFDALTDMALGPLRWPKKPLVLARFGLRALRP
                     FEEFARSEFSGRGLQALLAGIAAHAMRPLDELATNSFALVLGGAAHAVGWPLTRGGSS
                     SITSVLVELLRSLGGDVELGTEVRRFEDLPAARAYLFDVTPRQLLSIAGSRLPAGYRR
                     RLERFRYGAGIFKVDWALSEPIPWRDPSCARAATVHLAGDLDAIAKGESLIHDGRVPE
                     EPFIILVQPTLFDDTRAPVGKHIAWAYCHVPHRDLTDRTAAIELAIERFAPGFRDVVL
                     ARATRNSAELEAYDPNYVGGDINGGSAEWRQLFFRPVLSADPYATGAPDVFLCSSSTP
                     PGGGVHGMCGYFAAKSALAHVFGRRPSDNLDFDRER"
     gene            complement(5382..6110)
                     /locus_tag="VFQ35_19045"
     CDS             complement(5382..6110)
                     /locus_tag="VFQ35_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932812.1"
                     /translation="MTRLRALLLVLALLGCEELHSPKCQAECQKTGRCTSKGGTCVAS
                     SDAECGRSLACQQSGACSLAGETCVGTQATCKTFEGCRSRGLCGVLNGVCAATDRAHC
                     RDTEGCRDAGRCTPVNGACSTRGDDCKSALTCKKYGRCTAEGDDCKVKSSADCVLGEM
                     CTLQGYCSFVDGRCQVTSNDDCKKTSACRRVGACTFRADTGHNRMEAVPGCVVGGPED
                     CKASESCRTDKNCNFKAGLCVKNL"
     gene            6293..8797
                     /locus_tag="VFQ35_19050"
     CDS             6293..8797
                     /locus_tag="VFQ35_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932813.1"
                     /translation="MGIHEALRRALGKDKLRSLGVILALGAALAIFLSQVNKHYLIEE
                     WLFWHYASYWLVCLAWVACVFGIGELLLRRVFRLELPFLERMLTTFALGSLGFYFATF
                     LLGVCQGYRPIAFFLLPIVLIALAQKEYPAIWRRCVRVFRARPMKRSPYRFLGVAFGF
                     IGLLMVYFLVLTPDNIQFDSRWKHMALAEDYAAHGGIRRSTEGWLFAARPHLVSYLYS
                     WAFLIPSPRLFDKMLLCAHLEYVSFLISTVLGIPVLVRRLVPKADPTVVWAARFLFPG
                     VLLYDSSVSGGTDHFGAAFAAPIAVALLGVLRDLDPRRVALLATLLAGPILIKETAAL
                     LLVPFPVLALGARWLQDLWRRRRAPEKATKQKPWLVPAIGLGVGLVVTSPLWLQNLLL
                     YGDPLYPTLAKFFPARPWSEAAAYRFKWYYAERQMWAPPRTWDGFVETLKTLFTYSFD
                     PNDWKALHKNVPVFGSLFTLLLLPLPFLRKTGRIWLMVGWVHLGIFAWYMVHHQDRYL
                     QGIVPVMSAVVAAVLIAIFRSAGALAKVAASLVVVAQVVWGGDVYFFQTHSMARSPIK
                     KVVDLLGSGFHGDHEARFDVERSMVNIGNELPKGARVLMHEVHPHLGLGHESVLDNYP
                     WQYGLEYGAQGSPEGVRALLRKRGITHVHYSPGKANGIATFAGAILFQEFMDSVATNA
                     RRVSGGILAAVPEEPTTAPFNDSVAVLTCNEVPPRGLYALKSLDVLAYGPKGEDYGAT
                     IAPAETLDDAVRLLDEAEYAIVETRCFRGQSPPQFDAFEPFVRRKRDPREMWRRKRGG
                     PDNAAEAPAPNPAPGPSPRRGSHAPEAPEPEEPGQE"
     gene            8926..>9654
                     /locus_tag="VFQ35_19055"
     CDS             8926..>9654
                     /locus_tag="VFQ35_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9932814.1"
                     /translation="MKLIIQIPCFNEAATLGIALAELPKQVPGFSSVEILIIDDGSAD
                     DTVRVARENGVHHVVGFRSNQGLASAFMLGIHSCLERGADVIVNTDADNQYCAADIPK
                     LVQPIVDGRADLVIGARPITEIAHFSPIKKLLQRFGSWVVRRVSNTDVADAPSGFRAI
                     SREAAISLNVFNGYTYTLETIIQAGQKGMSVVSVPIRVNGELRPSRLVRSIPNYVKRS
                     ILTMFRIFVVYQPLKFFLTVGLFPF"
BASE COUNT         1689 a         3260 c         2967 g         1738 t
ORIGIN      
        1 cccgagccac ccgacccgcc cgagccacca cgtgacgtgg aaccgctagc tccccctcta
       61 ccgcctaagc cgccgctggc gctgttgtcg tcgtcatcgc cgccacaacc tggcatggca
      121 gccaccaaag aaataatcgc aaccgcgaaa agcttacgag tcttcatgag tgtattcctc
      181 gcggcgtctg tttgcaggaa gcgttcctat cgacgcagcc atttggccgc agatccgccg
      241 aaacgtcact cggcccgagt cccatcggaa ttatcgagcc tgtacgtgag aaggaattgg
      301 atcggtgcgc gccgttcagc gcacgcgaag ctcgcagttt cgtcggccca aaactcgtga
      361 ccgccccgcg tacgttcatt cagcgagcga gcgagcgcgg cgtgggttcg ttggcgacga
      421 cgccgagcgc cgaagaacac gatccacgga cgtgtgtggt ggatagcgct gcgctgtcgc
      481 tcgcgaacgc gaagacgcgc gggcgcctcg atccgacttc ggcgcgcgat gtactaggtt
      541 cccgttcccc ggtacacggt ggaatgacgc gcagcgcggg ccgttcagaa tcgatgctga
      601 ttccgagcac gtccgcgagg aagctctcgt caccaaacgt ctcctcgcaa aacgcgttcg
      661 aacgaactcg caagatggcg cggtcacggg gcaccgaacc tgctggcgct ctgcccgcca
      721 agcgatccgg cgcagggaga gcgcgtgacc atcgaccttc tcaagacact gggacacccc
      781 tcgagcgaca acgcctcacg tggaaggagc tcgttcaacc accgtacagc aagctcgacg
      841 acgatgcttt cacgcgggta cgcgtgatcc tcatgaacgg gatcgaagcc ggcgcgcttc
      901 gcttccagca cctgtgcggc cgtatgaacc gtgagctcca gctcgacttg gctcgcattc
      961 ggcgcatcga gcaacaccaa cagacgatgg tgaattggct caatccgccc gatcagaacg
     1021 tcctcgaaac gaccatcggc ttcgaacagg tggcgatcga agtaacggcc gcaatcgcgc
     1081 tggcggagcc ggatccgtac cttgcgcagg tctatcgcta cggcttgctc gaagacttcg
     1141 atcacatgta ccgctactcc gcgttgatgg atcgcgtgga agggaaggac gccaacacgc
     1201 tgcttcaaag ctacaccgac atcttgcccg ggcggcccac ggcagtagaa caccgcgcac
     1261 ccgaggatga gctgcgcgct tgctacgaca agcgcaccgc gacgctgctg acgaagctcc
     1321 acgccaacac gctgatctcg ggagagttcc agacgcacga ctactacatg acggtagggc
     1381 cgacgtactc ggatccgctc gggcgccagc tctacgcgga aatcgcctcg atcgaagagc
     1441 agcacgtcac ccaatacgag tcgatcgtcg acgcgacgga gacgtggctc gagaagtggc
     1501 tcttgaatga agcgaccgaa gtctacaact actatagctg tgtcgagtcg gagaagaact
     1561 cgcgcatcaa ggcgatttgg gagcggttct tggactacga gctcggccag ctcgcgcacg
     1621 tgcgcacgct cttcgaacga gtcgagcggc gggacgcggc cgaggtgctc ggcaccaaga
     1681 tcccggggcc catcgcgtac gaaagccacc gtgagttcgt gcgagaagta ttgcgcagag
     1741 aggccaactt gcgctcggtc gggagcgcct tcgtgccggc gagcgaggag ccccagggag
     1801 gggccaccgc catttatcgg gctcacatga actctgccgg ctcgccttcg gagtccgtcg
     1861 cgactggcta ttcgtggagc cccgggaccg aactcagccg caagcaaccg cgcactggca
     1921 acggcgtcga agtcacacag ccactctgag catcaggcga gtgaacccaa acctcacatc
     1981 aggaatcagt caatgtctac acaagcagag ctcggattca accgcaccgg catcgccacc
     2041 tctccgaagc tgagcgccga aatggttcag gggacaacgg agttcacgtc gactttcgac
     2101 ggtgacggcg cttcgatcgc cgcggtacgt gaggagtatg cgcgcagcgg cgagaaagtc
     2161 ggcagcgtac ccccgcccct cacgctgggc ggagtggcaa gcgcagcggt gcaaggcatt
     2221 cgcggaacac acccagtgga gttcctcgac aagatcggcg cgcgcctcgg gttcgaacgt
     2281 accggagttc ggctctacga ggcgctgatt tccaaattcg agtcgtcggg cgggttcacc
     2341 ggtgggccaa cgcgccgcga gctcgaagaa atcatgcacg aggagtacct gcatttcgcg
     2401 ctgctcgtcg acaccattac gaagctcggg ggcgatccga cggtcgtgac gccgtcagcg
     2461 gacgttgcgg ccacgctgtc tcgaggcatc ttggacgtgc tggtagatcc gcgcacgact
     2521 ttcgtacaat cgctcgaagc gatattggcg gctgagctcg tcgacaacga cgcttgggag
     2581 atgctgatcc tgggcgcgcg ccaacgtggc gacgacgagc tcaccgcccg cttcgagtca
     2641 gcgcgtgcag acgaagacaa gcacctcgcg cgggtgcgcg cgtggctgac cgcggcacag
     2701 ggccgccccg tcggggcgtg agggcgcgca aaggagaatg ccatgcgcgt gcatcatctg
     2761 aactgcgtct cgacctgtcc gctcggcggt tggttcatgg atcaccggac tcgcgacgtg
     2821 ctagcgcgcg gcaggctcgt ttgtcattgt ctcctcgtcg aagtcggggg agagctcgta
     2881 ctgatcgaca ccgggttcgg gctgctcgac gtggcgaacc ctcagagcag gctgagcgcg
     2941 ttcttcctct cactcgtgag gcccgagttt cgcgaagaaa tgacggcggt gcgtcaaatc
     3001 gcgaagctcg ggctcgaccc gaaggacgtg cgacacatcg tgatgacgca cctcgatttc
     3061 gaccacgcgg gcggcctcga cgactttccg ggcgcccgcg tgcatctctt ggcgcgcgag
     3121 cacgagcacg caaccgcccg gcgcacctgg ctcgacaagc aacgctttcg cccccagcag
     3181 tggtccacgc aggcatcatg gcagctctac gaacccgcat cgggcgagag ctggttcggc
     3241 tttgcgcgag cgcagccgct cagtggtctt ccaccagaga tcctgctcat ccctctgtac
     3301 ggacacacgc tgggccacgc gggagttgca gtagcgcaag gcgaccactg gctactcgac
     3361 gccggcgacg cctacttctt tcaaggcgag atgaacctcg aacgcccgtg gtgcacgccg
     3421 gggctgcgtt tctaccaaac gatgatggag aaagatcgcg aagcgcgtct cacgaatcaa
     3481 gagcggctcc gcgaactgcg gcggagcgag tcccagcgtg tggaggtgtt ctgcagccac
     3541 gacgtgagcg agttcgaacg cctttctggg cactccgccg cgctaccagc ggaatacctt
     3601 ggacaaaccg gcgctccgac ctcgttcgcg cccgccgagt agagcgaggt catggcacga
     3661 ccctggcttg gagaaaggcc atggtcgatc tgaatggcgc agtcgtcgtg gtgacgggcg
     3721 catccagcgg gattgggcga gctgccgcca tcgagttcgc ccggcggggc gcgcgcctcg
     3781 tcctcgcagc ccggcggagc caggccctcg aaggaacggc acgaatttgc gagcgcgaag
     3841 gcggttcgcc cgttcggggc cggtgagcga atgcgcgact gcgaagccgt catcgtgggc
     3901 gccggaccca acgggctcgc cgccgcgatc gtgctcgctg ccgcaggcgt gaagcctctc
     3961 gtcctcgaac gcgcacagca gcccggcggt ggcgcgcgca cctcggagct cacgctgccc
     4021 ggcttcttcc acgatacctg ctccaccatc catccgctgg cgttcggctc accgttgttt
     4081 cggcggctgc gcctcgagcg gtacggcctc gccgcggtgc attcacccgc ggccctcgcg
     4141 cacatagtag acacgaacac ggtcgtaacc ctcgagcgct cgctcggcgc gatggtcggg
     4201 aagctcggcg gtgacggcac ggcgtaccac gatgtcttcg cgccgatcgc agagcacttc
     4261 gacgccctca ccgacatggc gctcggtccg ctgcgttggc cgaagaagcc gctcgtgctc
     4321 gctcgcttcg ggctgcgtgc gttgaggccg ttcgaagagt tcgcgcggag cgagttctcg
     4381 gggcgcggac tgcaggcgct gctcgcgggg atcgcggcgc acgccatgcg accactcgat
     4441 gaactcgcca cgaattcgtt cgcgctcgtg ttgggcggtg ccgcacacgc ggtcggatgg
     4501 ccactgaccc ggggcggttc gagctccatc acgagcgtcc tggtcgagct cctgaggagt
     4561 ctcggcggcg acgtcgagct cgggaccgaa gttcgtcggt tcgaggattt gccggccgcg
     4621 cgcgcgtacc tgttcgacgt aacgcctcgg cagcttctct ccatcgcggg ctctcggctg
     4681 ccagcgggtt atcggcgcag gctcgaacgc tttcgttacg gcgcagggat cttcaaggtc
     4741 gattgggcgc tctcggagcc tatcccctgg cgcgatccga gttgcgcgcg tgcggcgacg
     4801 gtgcacctcg ctggcgacct cgacgcgatc gcgaagggcg aatcattgat tcacgatggt
     4861 cgcgtgcccg aggagccttt catcattctc gtgcagccca cattgttcga cgacacgcgc
     4921 gcgccggtgg gaaaacacat cgcctgggcc tactgccacg tgccgcaccg cgaccttacc
     4981 gaccgcaccg ccgcgatcga gctcgcgatc gagcgctttg ctccgggctt ccgcgacgtc
     5041 gtgctcgccc gcgcgacgcg caactccgcc gagctcgaag cctacgatcc gaactacgtg
     5101 ggaggtgaca tcaacggcgg cagcgccgag tggcgccagc tgttctttcg ccccgtgctg
     5161 agcgcagatc cctacgccac cggcgccccc gacgtgtttc tgtgctcttc ctcgaccccg
     5221 cctggcggcg gcgtccacgg catgtgcggc tacttcgccg caaagagcgc tctcgcccac
     5281 gtgtttgggc gccggccgtc cgacaacctc gacttcgatc gcgagcgcta ggcggtaaga
     5341 ttttggcttg cagcagagcc gcgagctcgg gacgctgcta gctacaggtt cttcacgcag
     5401 agcccggctt tgaagttgca attcttgtcc gtgcgacacg actcggacgc tttgcagtcc
     5461 tcggggccgc cgacgacgca gcccggcacc gcctccatgc gattgtgtcc cgtgtccgct
     5521 cgaaaagtgc acgcaccgac gcgacggcat gcgctcgtct tcttacagtc gtcattcgag
     5581 gtaacttggc agcggccgtc gacgaagctg caatagcctt gcaaggtgca catctctccg
     5641 agcacgcagt cggcgctgct cttcacctta cagtcgtccc cctcggccgt gcaccggccg
     5701 tacttcttgc aggtcaaagc cgatttgcaa tcgtcgcctc gcgtcgagca cgcgccgttc
     5761 acgggagtgc aacggcctgc gtcgcgacac ccctccgtat cgcgacaatg agcgcggtcg
     5821 gtcgcggcgc aaacgccatt cagcactccg caaaggccac gcgaacgaca accttcgaac
     5881 gtcttgcacg tcgcttgggt accgacgcac gtctcgcccg cgagcgaaca cgcaccgctt
     5941 tgctgacaag ccaaggaacg gccgcactcc gcgtcggagc tcgcaacgca ggtcccgccc
     6001 ttcgacgtgc agcgcccggt cttctgacac tccgcctgac acttgggtga atgcagctct
     6061 tcacaaccga gtaacgcaag caccagcaag agcgcacgaa ggcgagtcat tcgcccgttc
     6121 ttactcgatt ggcttcggct cgaccctcaa acggcctcga aaccgctctt tgctgcgtcg
     6181 gcccgatggc cagagcacgt ccatgaacgc ctgagcgcgc accaatacgg gacgctcttg
     6241 gccgccaagt ccattcccca gcgacgcccg ccgaggtaga gccaaggcga acatgggcat
     6301 ccatgaagcg cttcgccgcg ccctcggaaa agacaagttg cgttctcttg gcgtcatcct
     6361 ggcgctcggc gccgcgctcg cgatctttct gagccaggtc aacaagcact acctcatcga
     6421 agagtggctg ttctggcact acgcgagcta ctggctcgtg tgtttggcct gggtcgcgtg
     6481 cgtgtttggc atcggcgagc tgttgctgcg tcgggtcttt cgcctcgagc tgccgttctt
     6541 ggaacgcatg ctgaccacgt tcgcgctcgg ctcgctcggc ttctactttg cgaccttctt
     6601 gctcggcgtg tgtcaggggt accgaccgat cgcgttcttt ctcctgccga tcgtgctgat
     6661 tgccctcgcc cagaaggagt accccgccat ctggcggcgc tgcgtgcgcg tgttcagagc
     6721 gcgcccgatg aaacgctcgc cctaccgatt cctcggagtc gcgttcggct tcatcggcct
     6781 tttgatggtc tatttcttgg tgctcacgcc ggacaacatt cagttcgact cgcgttggaa
     6841 gcacatggcg ctcgccgagg actacgcggc gcacggtggc attcggcgtt cgaccgaggg
     6901 ctggttgttc gcggcccgcc ctcacctcgt gagctacctt tattcgtggg cgttcctcat
     6961 cccttcgccg cggctcttcg acaagatgtt gctgtgcgcg cacctcgagt acgtgagctt
     7021 cctcatctcg acggttttgg gcattccggt tctggttcgc cgcttggtcc caaaggccga
     7081 tccgactgtg gtttgggcag cgcgcttctt gttccccggc gtgctcttgt acgacagctc
     7141 cgtgagcggc ggcacggacc acttcggagc cgcgtttgcg gccccaatcg cggtcgcgtt
     7201 gctcggcgtg ttgcgcgacc tcgatccacg gcgagtcgcg ttgctcgcga ctctgctggc
     7261 gggacccatc ctcatcaagg agacggctgc cctcttgcta gtgccgtttc cagtgctcgc
     7321 cctcggcgcg cgctggctgc aagatctgtg gaggcggcgg cgcgcacccg agaaggcgac
     7381 gaagcaaaag ccctggctcg tcccggcgat tgggctcggc gttggcctcg tcgtgacgag
     7441 cccgctgtgg ctccagaact tgctgctcta cggcgacccc ctctacccga ctctcgcaaa
     7501 gttcttcccg gcgcgtccct ggtccgaagc cgctgcgtac cgcttcaagt ggtactacgc
     7561 ggagcggcaa atgtgggcgc ctccgcgcac ttgggacggc ttcgtcgaga cgttgaagac
     7621 gctgttcacc tattcgttcg acccgaacga ttggaaggcg ctccataaaa acgttccggt
     7681 gttcggttcg ctcttcacgc tgctgcttct gccgttgcca ttcttgcgaa agacgggtcg
     7741 catctggctg atggttgggt gggtgcacct cgggatcttc gcctggtaca tggtgcatca
     7801 ccaggatcgc tacctccaag gcatcgtgcc ggtgatgtcg gcagtcgtcg cggcggtgct
     7861 catcgcgatt ttcaggagcg cgggagcact ggcgaaagtc gcagcgagcc tcgtcgtggt
     7921 cgcacaggtc gtgtggggcg gggacgtgta cttctttcag acccactcga tggcgcgctc
     7981 gccgatcaag aaggtggtcg atctactcgg ctcgggtttc cacggcgatc acgaagcgcg
     8041 cttcgacgtc gagcgctcga tggtgaacat cgggaacgaa cttcccaaag gcgcgcgcgt
     8101 actgatgcac gaagtccatc cgcacctcgg gctcggtcac gagtcggtgc tggacaacta
     8161 tccgtggcag tacggcctcg agtacggcgc gcagggttcg cccgaaggcg tccgcgcgct
     8221 gctgcgcaaa cgcggcatca ctcacgtgca ctactcgccg ggcaaggcca acggcatcgc
     8281 gacgttcgcg ggagccatct tgttccagga attcatggat agcgttgcga cgaacgcgag
     8341 gcgagtcagc ggtggcatcc tcgcggccgt acccgaagaa cccacgaccg caccgttcaa
     8401 cgactccgtc gccgtcctca cctgcaacga agtcccaccg cgtggcctct atgctttgaa
     8461 gtcgttggac gtcctggcgt acggccccaa gggcgaagac tacggcgcca cgatcgcccc
     8521 cgccgagacc ttggacgacg cagtgcgcct gctcgacgaa gcggagtacg cgatcgtcga
     8581 gacacgctgc ttccgcggcc aatcaccgcc ccagttcgac gccttcgagc cgttcgtccg
     8641 gcgaaagcgc gacccccgcg aaatgtggcg ccgcaaacgc ggcgggcccg acaacgccgc
     8701 cgaagcgccc gccccgaacc ccgcaccagg cccttctccc cggcgcggaa gccacgctcc
     8761 cgaagcgccc gagcccgagg aaccagggca agagtaggcg acgagcctga agacgggcct
     8821 cggttcactg ccggctgacg ctgccctccg ctcgtcagcc gctcagaact ttcgtgcgga
     8881 accgagcgcg ccgaggcaca atccgccgcg ctcgcgcgaa ctgccatgaa gctcatcatc
     8941 cagatcccct gcttcaacga ggccgcgacc ctcggcattg ctctcgccga gctgcccaag
     9001 caagtcccgg gtttcagcag cgtagagatc ctcatcatcg acgacggcag cgccgacgac
     9061 accgtgcgcg tggcgcgcga aaacggcgtc caccacgtgg tcggcttccg ttccaatcag
     9121 ggcctcgctt cggccttcat gctgggcatc cactcgtgcc tcgagcgcgg cgccgacgtc
     9181 atcgtcaaca ccgacgccga caaccaatac tgcgccgccg acatcccgaa gctcgttcaa
     9241 cccatcgtcg acggccgcgc cgatctcgtc atcggcgcgc gacccatcac cgagatcgcg
     9301 cacttttcgc caatcaaaaa gctcttgcag cgcttcggta gctgggtcgt tcgccgcgtc
     9361 agcaacaccg acgtcgccga cgctccgagc ggcttccgcg ccatttcccg cgaagccgcc
     9421 atctcgctca acgtcttcaa cggctacacg tacacgctcg aaaccatcat tcaagccgga
     9481 caaaagggca tgagcgtcgt ctccgtgccc atccgcgtca acggcgagct ccgtccttcg
     9541 cggctcgtgc gcagcatccc gaactacgtc aagcgctcca tcttgacgat gttccgcatc
     9601 ttcgtcgttt accagccgct caagttcttc ctcaccgtcg gcctcttccc gttc
//
LOCUS       DASTKS010000911         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_815786, whole genome shotgun sequence.
ACCESSION   DASTKS010000911 DASTKS010000000
VERSION     DASTKS010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815786"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFQ35_19060"
     CDS             complement(<1..925)
                     /locus_tag="VFQ35_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932815.1"
                     /translation="MINPTCVVSRNVTRVSTVRSAGRAILLSLLCASALSAAACSAES
                     GGKLQQNTNERSDGSLQGSDVDSFAGAQATKGSARLDKPYLSGTCTQEATATLKDGSD
                     RIVAVTFSNQTYGGDRNDPNYVKDRTAGRLIGDAKSTTPKCYLHVPMKVTAGYKLSPT
                     DLIVRGFAHRGFIYSAYRWANAAQPSPANGWDVTNDGYTFERQVPAQEPPNQGGDNFT
                     IVEPLYNLWSPTCSTDKGAEVSAELIVTLQPYTAAGTNDTIVAVDSLDVTTFGPIEKC
                     NAPINYDPVAQEGESCGVIDNNTDRPVRCDTG"
     gene            complement(1137..1580)
                     /locus_tag="VFQ35_19065"
     CDS             complement(1137..1580)
                     /locus_tag="VFQ35_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932816.1"
                     /translation="MTSGIELDRSRYPLVLMRAGATYTQADWNDLMKGMIELIRRGPF
                     GLINDTRGSALPDPLQRRSIAQMYTDYELDVRRNFLASAIVGSSSFVNGVITALNWLK
                     PTPHPVKVFASLESAENWVLSHFSNDMRQSVTQAAAPRAAVPPST"
     gene            1952..>2564
                     /locus_tag="VFQ35_19070"
     CDS             1952..>2564
                     /locus_tag="VFQ35_19070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932817.1"
                     /translation="MNLQSNALEGQVLAGRYRVGALLGQGGMGMVVAAQHLLLDEPVA
                     IKFLAADALTFESLARFEREARAALKIKSEHVVRVLDVGRLDSGAPYLVMEYLDGEDL
                     GRRLRRLGRLSIEEAVDFVLQTCVAMADAHAIGIVHRDLKPSNLFCVRRSDGRAVIKV
                     LDFGISKQQGAETSFGEQLVTRTGAMLGSPLYMSPEQVQGARHV"
BASE COUNT          430 a          772 c          862 g          500 t
ORIGIN      
        1 ggccggtgtc gcatcggacc ggccgatccg tattgttgtc gatgacgccg cacgattcgc
       61 cttcttgcgc gaccggatcg tagttgattg gtgcgttgca cttttcgatc gggccgaagg
      121 tcgtgacatc gagcgagtcg acggcaacga tcgtgtcgtt cgtgcccgcg gccgtataag
      181 gctgaagcgt gacgatgagc tcggcggaaa cctcggcgcc cttgtcggtc gagcaagtcg
      241 gcgaccacag gttgtacaac ggctcgacga tggtgaaatt gtcaccgccc tggtttggcg
      301 gctcttgtgc cggaacttgg cgctcgaacg tgtacccgtc gttcgtcacg tcccagccgt
      361 tggcggggct tggctgcgcg gcgttggccc aacggtacgc ggagtagatg aagccgcggt
      421 gggcaaagcc gcgcacgatg aggtcggtcg gcgagagttt gtagcccgcg gtgactttca
      481 tcggcacgtg caagtagcac ttcggcgtcg tcgatttcgc gtcgccgatc agccgccccg
      541 ccgtgcgatc tttcacgtag tttggatcat tgcgatcgcc gccataggtt tgattgctga
      601 acgtcacggc tacgatacgg tcgctaccgt ccttcaaggt cgcggtggct tcttgggtac
      661 acgtgccgct gaggtatggt ttgtcgagtc gcgcggagcc cttcgtcgct tgcgctcccg
      721 cgaaggagtc gacgtcgctg ccttgaagcg agccgtcgct ccgttcgttg gtgttctgct
      781 gaagcttgcc cccactctcg gcgctgcaag cggctgcgga gagcgctgac gcgcacaaaa
      841 gcgacagcag gatggcgcga ccggcgctgc gcacggtcga gacgcgggtg acatttctag
      901 aaacgacgca tgtagggttg atcatggctt cctcaatccc gttcgacgca actcacaggc
      961 acgagcgtgc cccgtggggg cgcagcgcct gatgacgcgc ccactacgac cggcgagcgt
     1021 cgggagcgct gcgcctcaat ccgacaagtg tcgaacgtcg cgagaccggg tgacgcgaat
     1081 gagcgcgcgc cggtaattgg gggacatgcg ggccgagtcg aacgggccgc gcggcttcac
     1141 gtcgacggag gcacggcggc tcggggcgcg gctgcctgag tgaccgactg ccgcatgtca
     1201 ttcgaaaagt gggagagcac ccaattttcc gcggactcga gcgaggcaaa gaccttgact
     1261 ggatgcgggg tcggcttcag ccagttgagc gccgtgatga cgccattgac gaagctcgag
     1321 cttcccacga tggctgatgc caagaaattc ctccggacgt ccagctcgta gtccgtatac
     1381 atctgcgcga tcgagcggcg ctgcaggggg tccggcagag cgcttccgcg cgtatcgttg
     1441 atgagtccga acgggccgcg cctgatgagc tcgatcatgc ccttcatcag gtcgttccag
     1501 tcggcttggg tgtaggtcgc gccggcgcgc atgagcacca agggatagcg agagcggtcg
     1561 agctcgattc cactagtcaa ggtgaccccg cgtcttcctc tgccgtgcgc tcccgggcgc
     1621 tgcggactcc ttgaatttgc cacaggtttt ctcgccggcg cacctggcgg ttaacgctcg
     1681 tgagaattgg gcctccgaaa taccgcgacg gcctccagcg ctctcatgcc cgccatcgcc
     1741 ggacccctga gcctgcactg cgagcgtacc tttggccact tttcgtcgcc ctgacccaag
     1801 cccgagcgtt gaacacgggg cccttgcgaa acaggagccc acgagttcac gccggccatc
     1861 cgcgcggggg accagggcac aaataaaggc gtggccgccg aaccttctgc gcggctcgcg
     1921 cattgtccgc aaaggaaccg tctcatccaa ggtgaatttg cagagcaacg cgctcgaagg
     1981 tcaggtgctt gccgggaggt atcgggtggg cgccctgctc ggccagggag gcatgggcat
     2041 ggtggtcgcc gcccagcatc ttcttttgga cgagccagtc gccatcaaat ttctggcggc
     2101 cgacgcgctc acgttcgagt cgctcgcgcg tttcgagcga gaggcgcgcg cggcgctgaa
     2161 gatcaagagc gagcatgtgg tgcgcgtgct cgacgttggg cgtttggact cgggcgcgcc
     2221 gtacctcgtc atggagtact tggacggcga agatctcggt aggcgcttgc gacgactcgg
     2281 gcggttgagc atcgaagagg cggtcgactt cgttttgcag acttgtgttg cgatggccga
     2341 cgcgcacgcg atcggcatcg ttcaccgcga tttgaagccg agcaacttgt tttgcgtgcg
     2401 tcgcagcgac ggtcgcgcgg tcatcaaggt gctcgatttc ggcatctcca agcaacaggg
     2461 cgccgagacg agcttcggtg agcagctcgt cacgcgcacg ggcgcaatgt tgggcagtcc
     2521 gctctacatg tcgccggagc aggttcaagg cgctcgccac gtgg
//
LOCUS       DASTKS010000912         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1039574, whole genome shotgun sequence.
ACCESSION   DASTKS010000912 DASTKS010000000
VERSION     DASTKS010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039574"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFQ35_19075"
     CDS             <1..613
                     /locus_tag="VFQ35_19075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932818.1"
                     /translation="AMDLHAQLLLREPNLAPRVKESLQEIREEVRSLLRLVLNLLDIS
                     KSEEGQLAPRKADVNLIELSQTVLDAMQVKAKAGSVSLASQIGVERVVADPDLLRRVL
                     ENLLDNALRHAPENSTVRLTASPREHTVEIRVQDEGPGVADDMREKIFERFVQIESGA
                     RIVTRTGRGLGLAFCKLAVEAHGGHIWIEDAHPGAVFGFDLPK"
     gene            complement(635..2014)
                     /locus_tag="VFQ35_19080"
     CDS             complement(635..2014)
                     /locus_tag="VFQ35_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9932819.1"
                     /translation="MASRLRILVVDDDASARSALKKLFESQSFDVDVAADGKAGLERY
                     NEMPPDVIVTDLHMPELDGIGLLQGVREKDRSIPVIMVTSASELAPAVAAMRAGATDY
                     ITKPIDFDEVLLAVNRAVEQRDIKVENENLRRQLREQNGEGIQGLLGTSPAMQKVYRL
                     ARQVAPSRATVLVTGESGTGKGELARAVHALSPRADKPFVSVHCASLAETLLESELFG
                     HERGSFTGADRRRIGRFEQASGGTLFLDEIGEIPAATQVKLLTVLQERKIERVGGNEP
                     IPVDVRIIAATHRDLATDVQNGTFREDLFYRLNVVGIEMPPLRLRGGDMLVLATHFLQ
                     RFSLENHKSIEGFSERAKAKIRSYRWPGNVRELENAIERAVVLCDESLIQEEHLPHGS
                     TGAPSLDGVAVPGSTMAELEKYAILKTLEAVDGSTVRAAEILDISVRTIQYRLHEYGM
                     SKGGKAKGE"
     gene            complement(2253..2684)
                     /locus_tag="VFQ35_19085"
     CDS             complement(2253..2684)
                     /locus_tag="VFQ35_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932820.1"
                     /translation="MWVALALFVALHGLIHVMGFAKAFGASSLPLTTPIGKPLGVAWL
                     VTAVLMLSGAVLIVVRAESWWLPLVPGLLLSQGLILSAFADAKFGTIANLLIAIAIAS
                     SFAARRNPNIPSTSSVDPSAAAGNPGGAYPRSFGFPRQADS"
     gene            complement(2833..>4964)
                     /locus_tag="VFQ35_19090"
     CDS             complement(2833..>4964)
                     /locus_tag="VFQ35_19090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9932821.1"
                     /translation="RTRTIRSTLHEVEITLVITTIMVILVMGLFLRQLSATLIVGAVL
                     GVALISTCAAMYMLGFSLNNLTLVALVIAVGFVVDDAIVVTENITRHLEEGKSPMDAA
                     LTGARQIGFTILSITASLIAVFIPILMMGGVVGRLFREFAVTLSLAIAISAVISLTLT
                     PMLCSRWLRPAHREQNRVQRWLERAFDSLQAGYVRMLEWVLSHQRVMQLLTLGTLAAS
                     VALFVAVPKGLFPQQDTGLLMGFVQAPQDVSFRTMRERAEQIGKVVQADPDVDQVVQF
                     IGGGNATTNTGTVFVGLKPKPARKSTPDEIIARLRPKLGKVSGINLYMQAAQDLRVGG
                     RASRTQYQYTLQDVDLAELTTWSPRLLETLKQLPELRDVATDQQNAGLELRLNLDRDT
                     AARVGVSPQLFDNVLYDAFGQRQVATTFTALNQYRVVMEVQPEFAKSPADVDRLYVGT
                     PTGQVPLRALAHSEIRNAALAVNHQGQFPSVTLSFNLAPGVALGQAVDAIHAAERRMG
                     LPPTVRASFQGTAQAFRDSLASQPILIGAALLTVYIVLGVLYESFAHPFTILSTLPSA
                     GLGALLALLLTNSELNVIALIGIVLLIGIVKKNAILMIDFALEAEREHHLSSLDAVRE
                     ACRLRFRPILMTTLAALLGAVPLAFGSGAGAELRRPLGLTIIGGLLVSQLLTLFTTPI
                     VYLTLARISERRRARKARREELVSAVN"
BASE COUNT          913 a         1535 c         1641 g          875 t
ORIGIN      
        1 tgcgatggat ttgcacgcgc agctcttgct ccgcgagccg aacctcgccc cccgcgtgaa
       61 agagtcgctg caggagatcc gggaggaggt tcgctcgttg ctccgcctcg tcctgaacct
      121 gctcgacatc agcaagagcg aggaggggca gctcgctccg cgcaaggccg acgtcaactt
      181 gatcgagctc agccaaaccg tgctcgacgc gatgcaggtc aaagcgaagg ccggcagtgt
      241 ctcactggcg agccaaatcg gtgtcgagcg cgtcgtcgcc gatccggacc tcttgcgccg
      301 ggtgctcgag aacctactcg acaacgcgct ccgccatgcg cccgaaaaca gcaccgtgcg
      361 gctcaccgcg tcgccgcgcg agcacaccgt cgagattcgg gtgcaagacg agggccccgg
      421 tgtggccgac gacatgcggg aaaagatttt cgagcgcttc gttcagatcg agagcggcgc
      481 gcgcatcgtg acgcgcacgg gtagagggct cggcctagct ttttgcaagc tcgcggtcga
      541 ggcgcacggc gggcacattt ggatcgagga tgctcacccc ggcgcggtct ttggcttcga
      601 cctcccgaag tgagccgttc cctcgccgag ggcgctactc gcccttcgcc ttgccgccct
      661 tgctcatccc gtattcgtgc agacgatact gaatcgtgcg cacgctgata tcgaggattt
      721 ccgcggcgcg caccgtcgag ccgtccactg cctcgagcgt cttcaagatg gcgtacttct
      781 ctagctccgc catcgtcgag cccggaacgg cgacgccgtc gagagatggc gcgcccgtcg
      841 agccgtgcgg caaatgctct tcttggatga ggctttcgtc gcataggacg acggcgcgct
      901 cgatggcatt ttcgagctcg cgcacgttgc cgggccagcg gtagctgcga atcttggctt
      961 tggcgcgctc gctgaagccc tcgatgctct tgtgattctc gagcgaaaag cgctggagaa
     1021 agtgggtggc gagcacgagc atatcgccgc cgcgcaggcg gagcggcggc atttcgatgc
     1081 cgaccacgtt gagtcggtag aacaagtcct cgcggaaggt gccgttttgc acgtcggtcg
     1141 cgagatcccg gtgcgtggcc gcgatgatgc gcacgtcgac gggaatgggc tcgttgccgc
     1201 cgacgcgctc gatcttgcgc tcttgcagca cggtgagcag ctttacctgc gtggcggctg
     1261 ggatttcgcc gatttcatcc aggaacagag tgccaccgct cgcttgctcg aagcgaccga
     1321 tacgtcggcg atcggcaccg gtgaaggagc cgcgctcgtg gccgaagagc tcgctctcga
     1381 gcagcgtttc ggcgagcgac gcgcagtgga ccgaaacgaa cggcttgtcc gcgcgcgggc
     1441 tcagcgcgtg cacggcgcga gcgagctctc cctttccggt tccgctctcg cccgtcacga
     1501 gcacggttgc gcgcgagggt gctacctgtc gcgcgaggcg atacaccttt tgcattgcgg
     1561 ggctcgttcc gagcaagccc tggatgcctt cgccattctg ctctcgcagt tggcgacgca
     1621 ggttttcgtt ctcgaccttg atgtcgcgtt gctcgacggc gcgattgacc gcgagcagca
     1681 cctcgtcgaa gtcgatgggt ttggtgatgt agtcagtggc gccggcgcgc atggcggcaa
     1741 ccgccggtgc cagctcgctc gccgacgtga ccatgatgac ggggatgctg cgatccttct
     1801 cgcgcacgcc ttgcagcaac ccgatgccgt ccagctcggg catgtgcaag tcggtcacga
     1861 tcacgtcggg cggcatctcg ttgtatcgct cgagccccgc cttgccatcg gcagcgacgt
     1921 cgacgtcgaa gctctgcgac tcgaagagct ttttcagggc cgaacgagcc gacgcgtcat
     1981 cgtcgacgac caggattctc aaccgggaag ccatgccgtt ctctagtctc acagagtcga
     2041 acctttctgc aagtcgggtg gctggtcgta gtcgcgcgaa cgcgattcca ggctcctttg
     2101 cgagacccct cggctcgcac gcccccagga gccttcagcc cgacttgctg gcttcggtcc
     2161 tgaaaattca cgactgcctt caaagatcgt cgccccgccg tgcgccagtg agcgggcagt
     2221 ggcgctcaca tatcgattgt acatttcgaa gctcacgagt ccgcctgacg ggggaagccg
     2281 aaactgcgtg gatacgcgcc gcccgggttc ccggccgcgg ccgatggatc taccgaagag
     2341 gtagagggga tgttcgggtt tcgccgtgcc gcgaacgagg acgcgatagc gatcgcgatg
     2401 agcaggttcg cgatcgtgcc gaacttggca tccgcgaacg cactcaggat gagcccttga
     2461 gagaggagca gccccgggac gagcggaagc caccacgact ccgcacgcac gacgatgagc
     2521 acggcacccg acaacatcaa tacggcagtg acgagccacg cgacgccgag tggcttgcca
     2581 atgggggtgg tgagtggcaa cgagctcgcg ccgaaagcct tcgcgaaccc catcacgtgg
     2641 atgaggccgt gcagggcaac gaagagagcg agggcaaccc acatgcggcc caagcccttc
     2701 gcaagagcta tgccgcgcac actccaagga acgcaaccga ttttgcgcag ttatcgcctg
     2761 cagaatttgc gcgatgcacg aatttcgcgc aaagactgct gcgcaaaacg tcggaccggt
     2821 gcttggctgc cctcagttca ccgcgctgac gagctcttca cgccgggctt tgcgtgctcg
     2881 ccggcgctcc gaaatgcgag cgagcgtgag atacacgatg ggagtcgtga acagcgttag
     2941 aagctgtgaa accaagagtc cgccaatgat ggtgagcccg agcggacgcc ggagctccgc
     3001 cccagccccg ctaccgaacg cgagcgggac cgccccgagc agggccgcga gggtcgtcat
     3061 gagaattggc cggaagcgga ggcgacaggc ctcgcggact gcatcgagcg aggacaagtg
     3121 atgctcacgt tcggcctcga gcgcgaaatc aatcatcaaa atcgcgttct ttttcacgat
     3181 gccgatcaga aggacgatgc cgatgagggc gatgacgttg agctcgctgt tggtgagcaa
     3241 gagcgcgagc agcgctccca gccctgcaga gggcaacgtt gaaagaatgg tgaaggggtg
     3301 tgcgaagctt tcgtacaaaa ccccgaggac gatataaacg gtgagtaacg ccgcgccgat
     3361 caagatgggt tggctcgcga gcgaatcgcg aaaggcctga gccgttcctt gaaacgacgc
     3421 gcggacggtg ggcggcaggc ccatgcgtcg ctctgcggca tggatggcgt ccacggcttg
     3481 cccgagcgcg actccgggcg ccaaattgaa agacagcgtg accgacggaa attggccctg
     3541 gtgattcacg gccagagccg cattccggat ctcgctatgg gcaagcgcac ggaggggaac
     3601 ctgcccggtc ggcgtgccca cgtagagtcg gtcgacgtcg gccggtgact tcgcgaactc
     3661 gggctgcacc tccatcacga cgcggtactg attgagggcg gtgaaggtgg tcgcgacttg
     3721 gcgctgcccg aacgcatcgt agagaacgtt gtcgaagagt tgggggctca ctccgacgcg
     3781 ggcagcggtg tcgcgatcga ggttcaatcg gagctcgagt cccgcgtttt gctggtcggt
     3841 cgccacgtca cgcagctccg gcagctgctt caaagtctcg agtagacggg gggaccaggt
     3901 cgtgagttcg gcgagatcga cgtcttgcag cgtgtactgg tactgcgtgc gcgacgctcg
     3961 cccgccgacg cggagatcct gcgccgcttg catgtagagg ttgatgcccg agactttgcc
     4021 gagcttcggc cgaagccgcg cgatgatctc gtcgggcgtg ctcttccggg cgggtttcgg
     4081 cttcaatccg acgaacacgg tgccggtgtt ggtggttgcg ttgcccccgc cgatgaactg
     4141 cacgacctgg tcgacgtcag ggtccgcttg cacgactttg ccgatctgct cggcgcgttc
     4201 gcgcatggtt cggaacgaga cgtcctgcgg cgcttgcacg aaacccatca agagccccgt
     4261 gtcttgctgc gggaataggc cctttggtac ggccacgaac agcgcgacgc tggcggcgag
     4321 ggtcccgagc gttaagagct gcatcacgcg ctggtgggag agcacccact ccagcatccg
     4381 gacgtaccca gcttggagtg agtcgaaggc gcgctcgagc cagcgctgga cccggttctg
     4441 ctctcgatgc gccgggcgaa gccaacgtga gcacaacatg ggcgtgagcg tcagcgagat
     4501 caccgccgaa atcgcgatcg cgaggctgag cgtcacggcg aactcgcgga agagccgccc
     4561 cacgacccca cccatcatca agatgggaat gaacacggcg atgagcgagg ccgtgatgga
     4621 gaggatcgtg aagccaattt gtcgcgctcc ggtgagggcc gcgtccatcg gcgacttccc
     4681 ctcttcgagg tgccgggtga tgttctcggt cacgacgatg gcgtcgtcga cgacgaagcc
     4741 cacggcgatg accagcgcca ccagggtcag gttgttgagg ctgaatccca gcatgtacat
     4801 ggcggcgcag gtcgatatga gcgccacgcc caataccgcg cccacgatca gggtcgcgga
     4861 caattgccgc aggaacaggc ccatcaccag gatcaccatg atggtggtga tcaccagcgt
     4921 gatctcgacc tcgtgcaggg tcgagcggat ggtgcgcgtc cggt
//
LOCUS       DASTKS010000913         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1934431, whole genome shotgun sequence.
ACCESSION   DASTKS010000913 DASTKS010000000
VERSION     DASTKS010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934431"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFQ35_19095"
     CDS             complement(<1..1085)
                     /locus_tag="VFQ35_19095"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9932822.1"
                     /translation="MTELRLYDSLRRSVQPFRPRVPGVATVYSCGPTVYSRQHLGNMR
                     PYVFADLLTRVVRLQGYDVRHVINITDVGHLTDDADAGEDKIERAAHASGASALDVAR
                     VWTELFKQDLKLLGVREPTVWAKATDHIPDQIAMIELLAEKGFTYLLPDGLYFDTSRD
                     PGYGALSGLRASAEHARVEGVGGKRSPADFALWKFSPTSGPRRQLEWQSPWGVGFPGW
                     HIECSAMAARYLGREIDIHTGGVDHIAVHHTNEIAQSENALGTRPWVRFWMHGAWLNL
                     DGEKIAKSRAHERKPPDLDDLAGFGVSPADFRYYLYTAHYRSPLALSGEALRGAQAAR
                     RRLGALARAAVPSIPDTLPTAETLRRLR"
     gene            1285..2061
                     /locus_tag="VFQ35_19100"
     CDS             1285..2061
                     /locus_tag="VFQ35_19100"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET9932823.1"
                     /translation="MRTAQRVLLEFLGEHEGISEPDVHGLLFRYCQGSAQPAYDFVLY
                     AERAHSFTAAADLEKLIQKGVVERDSGRLRLRASAPGTLPAAEPRVEHRHQPIGTPLS
                     HEASPSSGLCAPPLCTIGYEGRSLENYLNRLLTARVSTLCDVRRNPLSRKFGFSKGTL
                     SRACCAVGIEYEHLPELGIASEQRRHIVTDADRQALFREYAGTTLAAELVALDRIGAR
                     LEAGQRVALTCYEERPEDCHRNALARALSARFDVPSTAHL"
     gene            complement(2066..2998)
                     /locus_tag="VFQ35_19105"
     CDS             complement(2066..2998)
                     /locus_tag="VFQ35_19105"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9932824.1"
                     /translation="MATPADPSDAPLDVYVYGMTVLSTIHRLTSAPSDFDGYAEIAES
                     RVCPGGEGMNAALLLASLGLRVGLGGPHWGTETAARLERYARRHAIDVSSIERDDSFP
                     GVMDVVLVGGGRRLVLGAFQRFFSEERARWSEPSEASIRAARVVAIDPFFRESSERAA
                     ELSTRYQKPYVTIDCAYDGLLHSGARVNVVSREFRNQRYPSVDEQELFGRYVERARSG
                     GLVIFTSGSEPIQYWDGARSLFFQPYRVDAKSTLGAGDVFRAGVVFGVQQGLEAAECV
                     RFAAGLSALMCTRFPIADELPTRDDVERFLRERP"
     gene            complement(2982..3872)
                     /locus_tag="VFQ35_19110"
     CDS             complement(2982..3872)
                     /locus_tag="VFQ35_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932825.1"
                     /translation="MTAASVRWAVLALSLPLAVGCAASTPSATAASPATNPASAAPGQ
                     APGKSEPGASADCRPWATVVEGPYRYTNNVWGKDKAKGPFEQCLLSRTVEGRVERGWT
                     WSWPGFDPSVFSYPEIEFGWKPWNGGKSTDARFPMRVSDATQFRLNYEIETSVSGNFD
                     FAPEVWLLKERPSTESANPKLITTEIMFWLDYGGGARPAGRAIQKFALDGADYELWKE
                     DDIGKEANGQGWRLYSFKSLTVQRKGVLRIDAFLKHLVEQNLVNPDEFVAGVEFGNEV
                     MGGTGTTWVKRFEVEVGNSG"
     gene            complement(3958..4161)
                     /locus_tag="VFQ35_19115"
     CDS             complement(3958..4161)
                     /locus_tag="VFQ35_19115"
                     /inference="COORDINATES: protein motif:HMM:NF025143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HET9932826.1"
                     /translation="MGNVVNLNKFRKRKAKLEREQRAETNRRLHGRTAAERALDELRK
                     KQLNGKLEGAKLGPNSSDPEGNE"
     gene            complement(4214..>4963)
                     /locus_tag="VFQ35_19120"
     CDS             complement(4214..>4963)
                     /locus_tag="VFQ35_19120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9932827.1"
                     /translation="STAASIEHPMAKVVDPEVVKTLRRALVPFQELLKAPWLSSVTPA
                     RVPRVTDYLSIHFAEQAGTEASVLPPRAYDEKFHILEAPALALSDLAYYRRRCGIREA
                     SMGVVYADIDDFKAVNTKLGETEVDVRILPRFMELLEAWVFARGHAYRFGGDEYLLLL
                     PNSDRALCLNLLRDLLARLARREIVRGIRLNLSLGLCLVDPECLLTDREILQRANAAK
                     QAAKAAGKGRIGATVPPDHERIETLEPAPPS"
BASE COUNT          872 a         1635 c         1564 g          892 t
ORIGIN      
        1 cgcagccttc gcaaagtctc cgcggtgggc agtgtatccg ggatcgaagg gacggccgcg
       61 cgcgccagcg cgcccaagcg ccgccgagct gcctgcgccc cacgcagggc ttcgccggaa
      121 agcgcgagcg ggctccggta gtgcgccgtg tacaaatagt agcgaaagtc tgccgggctc
      181 acgccgaaac cagccaaatc atccagatcg ggcggcttgc gttcgtgcgc gcgcgacttc
      241 gcgatcttct caccgtccaa gttcagccag gcaccatgca tccagaatcg aacccacggc
      301 ctcgtcccca acgcgttctc gctctgcgcg atctcgttcg tgtggtgcac ggcgatgtgg
      361 tccaccccac cggtgtgaat gtcgatttca cgacctaggt agcgcgcggc catcgcactg
      421 cactcgatgt gccatccggg aaaaccgacg ccccaggggc tttgccattc gagctgacgt
      481 cgaggaccgc tggtaggcga gaatttccaa agcgcgaagt ctgccgggct ccgcttgccg
      541 cctaccccct cgacgcgcgc gtgctcggcg ctcgcccgta aacccgagag cgcgccgtag
      601 cccggatcgc ggctcgtgtc gaagtagagc ccgtcgggaa gcaggtaggt aaagcccttc
      661 tctgccagca gctcgatcat cgcgatctgg tccggaatgt ggtcggtcgc cttcgcccag
      721 acggtgggct cgcgcacgcc gagcagcttc aaatcctgct tgaagagttc ggtccagact
      781 cgcgcgacgt cgagcgccga ggcgccgctc gcgtgcgcgg cccgctcgat tttgtcctct
      841 cccgcgtcgg cgtcatcggt caggtggccc acgtcggtga tgttgatgac gtgccgcacg
      901 tcgtacccct gtagacgcac gacccgtgtc agcaagtccg cgaacacgta agggcgcatg
      961 ttaccgagat gctggcggct gtacacggta gggccacagc tgtacacggt cgcaacgccc
     1021 ggaacgcgcg ggcgaaacgg ttgcacggaa cggcgcaagc tgtcgtaaag ccgaagttct
     1081 gtcacgagct tttccttgtc caaagagtcg agcggcaagg gaaaagccgg ccgcccacgg
     1141 ctttgcggga cggcaggttc gaagcaggct cgcgtcgagt taggccgtcc gcgcgggacg
     1201 actacacagg acgaagagca ctgaccggaa catcgacggg gagtctagcg cttctctctc
     1261 agtctgtcac gctcccccac gtcgatgcga accgcacaac gtgtgctcct cgagttcctc
     1321 ggtgagcacg agggcataag cgaaccggac gttcacgggc tcctctttcg gtactgccaa
     1381 ggcagcgcgc agcccgcata cgacttcgtg ctctacgctg agcgcgccca ttccttcacc
     1441 gccgcggcgg atctcgagaa gctgatacag aagggcgtcg tggagcgcga ttctggccgc
     1501 cttcggctgc gggcctcggc tccgggtaca ctccccgcgg cggagcctcg cgtcgaacac
     1561 cggcatcagc cgatcggtac gccgctctcg cacgaggcga gcccgagctc ggggctctgc
     1621 gctccgccgc tctgcacgat tggctacgag ggccgcagct tggagaatta cctcaatcgc
     1681 ttgcttacgg cccgagtgag cacgctctgc gacgtgcgaa ggaacccgct gagccgcaag
     1741 ttcggctttt ctaagggcac tctgtcgcgc gcatgctgcg cggtcggcat cgagtacgag
     1801 cacttaccgg agctcggcat cgcctccgaa cagcgacgac acatagtgac cgacgcggat
     1861 cggcaggccc tattccgaga gtatgcggga accacgctcg cggccgagct cgtcgctctc
     1921 gaccgcatcg gcgctcggct ggaggcgggg cagcgcgttg ccctcacctg ttacgaagag
     1981 cgccctgagg actgccaccg caacgccctc gcccgggccc tctcggcgcg gttcgacgta
     2041 ccttccactg cccacctctg agcgttcagg ggcgctcccg aaggaagcgc tcgacatcgt
     2101 cgcgagtggg gagctcgtct gcgatgggaa agcgcgtaca catcaacgcc gagagcccgg
     2161 ccgcgaaacg aacgcactcc gcggcttcga gcccttgctg aaccccgaac accacgccag
     2221 ctcggaacac gtcccctgcc cccaacgtgc tcttcgcgtc gactcggtag ggctggaaaa
     2281 agaggctacg cgcgccgtcc cagtactgga tgggctcgga gcccgaagtg aagatgacca
     2341 gaccaccgct ccgcgcacgc tccacgtagc gaccgaatag ctcttgctcg tcgacgctcg
     2401 ggtagcgctg gttgcgaaac tcgcgggaga cgacgttgac cctagctccg gagtgaagca
     2461 ggccatcgta cgcacagtcg atcgtcacgt aaggcttttg gtagcgagtc gagagctccg
     2521 cggcgcgctc ggagctctcc cgaaaaaatg ggtcgatcgc gacgacgcgc gcggcgcgga
     2581 tcgatgcttc cgacggttcg ctccagcgcg ctcgctcctc cgaaaagaag cgctgaaaag
     2641 ctcccaggac cagccgacgt ccgccgccca cgagcactac atccatcact ccggggaagg
     2701 aatcgtcgcg ctcgatgctg ctcacgtcga tggcgtggcg tcgcgcgtag cgttcgagcc
     2761 gggctgccgt ttccgtaccc caatggggac caccgagccc gacacggagg ccgaggctcg
     2821 cgagcaagag cgcggcgttc atcccttctc cgcccggaca gacgcgactc tcggctattt
     2881 cggcgtaacc gtcgaagtcg gagggggcgc tcgtcagacg gtgaatcgtc gaaagcaccg
     2941 tcatgccgta gacgtaaacg tcgagcggcg cgtcgctggg atcagccgga gttgccaact
     3001 tcgacctcga agcgcttgac ccatgtcgtg ccggtgccgc ccatgacttc gttgccgaac
     3061 tcgacgcccg ctacgaactc gtccgggttc acgagatttt gttctactag gtgcttcaag
     3121 aatgcgtcga ttcgcagcac gccttttcgc tgcaccgtga ggctcttgaa cgaatacaag
     3181 cgccaaccct ggccgttcgc ctctttacca atgtcgtcct ctttccagag ctcgtaatcg
     3241 gcaccgtcca gtgcaaactt ctgaatcgct cggcccgcgg ggcgcgctcc gcctccgtag
     3301 tcgagccaga acatgatttc agtcgtgatg agcttggggt tcgcgctctc ggtagacggg
     3361 cgctctttca aaagccagac ctccggcgcg aaatcgaagt tgccgctcac gctcgtctcg
     3421 atctcgtagt tcagccgaaa ctgcgtagcg tcgctgacgc gcatggggaa acgcgcgtcg
     3481 gtgctcttgc cgccattcca aggcttccaa ccgaactcga tctccgggta cgaaaagacg
     3541 ctcggatcga agccgggcca gctccaggtc caccctcgtt cgacacgccc ctcgaccgtg
     3601 cgcgaaagca agcattgctc gaatggcccc ttcgccttgt ctttccccca aacgttgttg
     3661 gtgtagcgat acggcccttc cacgacggtg gcccagggtc gacaatcggc gctggcgccg
     3721 ggctcggatt tgccaggagc ctggccgggc gcggccgacg ccgggttcgt cgctggcgag
     3781 gcagcggtgg cgctcggcgt gctcgccgca cagccgacgg cgagggggag cgacaaggcg
     3841 aggacggccc agcgaacgga tgcagcagtc acgtgaagca tgccgagcta gatggggcga
     3901 gcgcttgcga ccgatcgggg aatccgcgcg attccgcaaa tatgccgcga cgccgcctca
     3961 ctcgttgcct tcggggtcgc tcgagtttgg accgagcttg gcgccttcga gcttcccatt
     4021 cagctgcttc tttctaagct cgtcgagggc gcgttcagcc gccgtccgcc cgtggaggcg
     4081 acggttggtc tcggctcgct gttctcgttc gagcttggct tttcgctttc tgaacttgtt
     4141 cagattgacg acgttgccca tggtcgcgag tctaccccga tcacggctcg aatccatctg
     4201 cactgacgct tctttaagag ggcggggccg gttcgagggt ttcgattcgc tcatgatcgg
     4261 gcggtacggt cgcgccgatg cgccccttac cggcggcttt ggccgcttgc tttgccgcgt
     4321 tggcgcgctg caaaatctct cggtccgtaa gcaaacactc ggggtcgacg aggcagagcc
     4381 cgaggctcaa attcagacga atgccgcgca ctatctcgcg gcgggcaagc ctcgcgagga
     4441 ggtcgcggag gaggttcagg cagagtgctc ggtcggagtt cggcaagagc aggaggtact
     4501 cgtcgccacc gaagcggtaa gcatggccgc gcgcgaacac ccacgcttcg aggagctcca
     4561 tgaagcgcgg caagatgcgc acgtccacct cggtttcacc gagcttcgtg ttcaccgctt
     4621 tgaagtcgtc tatatcggcg tagacgactc ccatactggc ctcgcgtatg ccgcagcggc
     4681 ggcgatagta agcgaggtcg ctcaacgcga gggcgggtgc ctcgaggatg tgaaacttct
     4741 cgtcgtaggc gcgtggcggc aggacgcttg cttcggtgcc cgcctgctcg gcgaaatgga
     4801 tcgacaggta gtcggtcact cgcggtacac gcgcgggcgt cacgctgctc aaccacggcg
     4861 ccttcaagag ttcttgaaaa ggcaccaacg ctcgtcggag cgtcttgacg acttcggggt
     4921 cgaccacttt ggccatcggg tgctcgatcg aagccgccgt gct
//
LOCUS       DASTKS010000914         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_147179, whole genome shotgun sequence.
ACCESSION   DASTKS010000914 DASTKS010000000
VERSION     DASTKS010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147179"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFQ35_19125"
     CDS             complement(<1..1147)
                     /locus_tag="VFQ35_19125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9932828.1"
                     /translation="MLILGKLVGWSLRNRPVVLLAAALLILIGARAAVRLPIDAVPDV
                     TNIQVQIITSAPALSPAEVEQYVTVPVERAMAGIPKTTEVRSISKYGISVVTVVFEDD
                     TNIYFARQLVNERMPEAMGAVPTQYGRPEMGPISSGLGEIFQFVVRNDRLSLMQLEEL
                     LDWQIGPVLRTVPGVVEVNSFGGEDKQYQVVLDPKRLQAAGVSVAQVIEALERSNANA
                     GGGYIEHAREQFVIGTAGLVRSLDDLRSVVIGATTEGVPITVATVGDVRFGPRLRRGA
                     ASMDGKGEVVVGVALMLLGENSRTVTENVKAKLRTLQPSLPEGTRVEPFYDRSLLVDR
                     TIRTVSRNLLEGALLVIGVLFLLLGELRAGVVVALTIPLSLLGAVIAM"
     gene            complement(1147..2322)
                     /locus_tag="VFQ35_19130"
     CDS             complement(1147..2322)
                     /locus_tag="VFQ35_19130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9932829.1"
                     /translation="MTALRFVTLALVMFACSRQSETPSKGDHDTRPSASAVAEEDKGH
                     EALPHVVKVAPEVVAKAGIKVAPVGRTVLAKALVLPGEIAADPDRLARISSPAAGRIE
                     AVRFREGETVKKGDALIVVRVPELAKVRGSYLATQSKAKAARSNAVRLRSLLDDRLAS
                     EQAVLDAEAEASSLELEARSGAEQLGALGAAASGAFAITLRAPLSGTVVARNAVMGQP
                     AAAEEVLGTIADLDEVWFLARVFEKDLGELTVGAPAEVHLNAYPDEHFSGTTEYIGQQ
                     IDPVARTVTARVRLHNRNGLLRLGLFGTSQISIAGGEAEPRLVVDTASVTDIGGKTVV
                     FVRESEGVYALHEVTVGTEAVGKVEVLAGLREGEQVVSDGVFTLKSMALKGTFSEEE"
     gene            complement(2319..>2631)
                     /locus_tag="VFQ35_19135"
     CDS             complement(2319..>2631)
                     /locus_tag="VFQ35_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932830.1"
                     /translation="ALLAAESRRRAKADLVAALGAYAAARETNAAYTSDRIQRAEAAL
                     VSLAAGVESSRVSTRDAVILQEPLFDLLLGAVEARRLLCISSVNLVYAAGLPMNREGV
                     R"
BASE COUNT          468 a          886 c          816 g          461 t
ORIGIN      
        1 tcatcgcgat gactgcgccg agcagcgaaa gggggattgt cagtgccacg acgacgcccg
       61 cgcgcagctc tccgagcagc agaaacagga cgccgatgac cagcaatgcc ccttcgagca
      121 gatttctaga gacggttcga atcgtgcggt caacgagcag cgagcgatcg taaaaaggct
      181 cgacgcgcgt cccctccggc aacgacggct gcaacgttcg aagcttcgct ttcacgtttt
      241 cggtcacggt gcgcgagttc tcgccgagca gcatgagcgc aacgccgacg accacttcgc
      301 ctttgccgtc catcgatgcc gcgccccgcc gcaagcgcgg accaaatcgg acgtccccca
      361 cggtcgcgac ggtgatgggt acgccttcgg tcgtggcgcc gatgacgacg ctccgcaggt
      421 cgtcgaggct gcgtacgagc cccgcggttc cgatcacgaa ctgctcccga gcgtgctcga
      481 tgtagccacc cccggcgttc gcgttcgagc gctcgagcgc ctcgatcacc tgcgcgacgg
      541 aaacgccggc ggcctgtagc cgcttcggat cgaggacgac ctggtactgc ttgtcctccc
      601 cgccgaagct gttcacctcg accacgcccg gcaccgtgcg caaaacggga ccgatctgcc
      661 agtcgaggag ctcctcgagc tgcatcaagg acagccgatc gtttctaacg acgaactgga
      721 atatctctcc tagacccgaa gaaatgggtc ccatctccgg ccggccgtat tgggtcggga
      781 cggcgcccat cgcttcgggc atgcgctcat tcacgagttg ccgcgcgaag tagatgttcg
      841 tgtcgtcctc gaagaccacc gtcacgacgg agatgccgta cttggagatg gagcgaacct
      901 ccgtcgtttt cgggatcccg gccatggctc gctcgactgg tacagtgacg tattgctcga
      961 cttcggcagg tgagagcgcg ggtgccgacg tgatgatttg gacctgaatg ttggtcacgt
     1021 ctggaacggc gtcgatcggc aaacggaccg cggcgcgtgc cccgatcaag atcagcagag
     1081 ccgccgcgag cagcaccacg ggtcggtttc tgagtgacca gccgacgagt ttcccgagga
     1141 tcagcatcac tcttcctcgg aaaaggtgcc cttgagcgcc atgctcttga gcgtgaagac
     1201 gccgtccgag acgacttgct cgccttcgcg taagccagca agcacctcga ccttaccgac
     1261 ggcttcggtg cctactgtca cttcgtggag cgcatagacg ccctccgact cgcgcacgaa
     1321 cacgaccgtt ttgcccccga tgtcggtgac gctcgcggtg tccacgacga gcctcggttc
     1381 ggcttcgccg cccgcaattg aaatctgact ggtcccgaac agaccgaggc ggaggagccc
     1441 gttccggttg tgaaggcgca cgcgagccgt caccgtgcga gcgaccggat cgatctgctg
     1501 cccgatgtac tccgtcgttc cggagaagtg ctcgtccgga taggcattca aatgcacctc
     1561 tgccggcgcc cccaccgtga gctctcccag atccttctcg aatacgcgcg cgagaaacca
     1621 gacctcatcg agatcggcga tcgtcccaag cacttcctcg gccgcggcgg gttgacccat
     1681 gaccgcgttt ctggcgacaa ccgtgccgct gagcggagcc ctcagcgtga tcgcgaacgc
     1741 gccggacgct gctgcgccga gcgcgccgag ctgttcggca cccgaccttg cctcgagctc
     1801 gagcgagctc gcctccgcct ccgcgtcgag cacggcttgc tcggatgcga ggcggtcatc
     1861 gagaagagat cgcaaccgca ccgcgttcga gcgcgccgcc ttcgccttcg actgcgtcgc
     1921 gagataggag ccacggacct tggcgagctc gggcacccgg acgacgatga gcgcgtcacc
     1981 cttcttgacg gtttctcctt cacgaaagcg caccgcctcg atgcgccccg ccgcggggga
     2041 cgagatgcgc gccaaacgat cgggatcggc ggcgatttcg ccgggcagca ccaacgcttt
     2101 ggcaagcacc gtgcgcccga ctggagcgac tttgattcca gccttggcga cgacttcggg
     2161 cgcgactttc acgacgtggg gaagcgcctc gtgacccttg tcttcctcgg cgacggcgga
     2221 cgcgctcgga cgcgtgtcgt ggtcgccttt cgatggtgtc tcgctctgac ggctgcaagc
     2281 gaacatgacg agcgctagcg tgacgaatcg caaagccgtc atcgcacacc ttcccgattc
     2341 attggcagtc cggcggcata gacgagattg acggaggaaa tgcagagaag tctccgcgcc
     2401 tcgaccgcac cgaggagaag gtcaaagagc ggttcctgga ggatgaccgc atctctcgtg
     2461 ctgacgcggc tgctctcaac tcccgcggct agactcacga gcgcggcttc agcacgctgg
     2521 attcggtcac tcgtataggc cgcgttcgtc tcgcgcgccg ctgcgtaggc gcccagagcg
     2581 gcgacgaggt cggccttcgc cctgcgccgg ctctccgccg cgagaagcgc g
//
LOCUS       DASTKS010000915         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_815966, whole genome shotgun sequence.
ACCESSION   DASTKS010000915 DASTKS010000000
VERSION     DASTKS010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815966"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFQ35_19140"
     CDS             <1..459
                     /locus_tag="VFQ35_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932831.1"
                     /translation="AGTGLSLDVWVGFGLSPGLVLGPAISYWGGNDGDPSISGGASSG
                     ESGSALLGAFVDAYPNPRRGEHFGGALALGTTSAKLSGRPEIKDYQGGGVGFLVFGGY
                     DFWIARTWSLGGLLKLSGLATRGNSTIDGQKVERQATSYAASIAVTLLYQ"
     gene            complement(440..1624)
                     /locus_tag="VFQ35_19145"
     CDS             complement(440..1624)
                     /locus_tag="VFQ35_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932832.1"
                     /translation="MWFASKSRRSQAALLTLGIASATAAQASPERWDPTVVREFSLAR
                     IRTSTRSSDFNLPLRPEVVWRVRVASAVPFAPTPTSDGSVVLGLATPVVAEYDARGRL
                     LWTARLGASNAATSLIVLGDGARMVLTQAGEAISFSPRGQVLRRHSLPLPALEAPPLV
                     APTPDGGMFIAAGRRVLQLDASLGVVATARADQDVRAILPAPSRSLIVLANGNVLELA
                     RNGGWRRVASFNARVDAAVRRGDDSVLALVDGRRLVELNVATQLPTTHFAETDVEFSP
                     LLVQNTPRGLRLMSNLDFVFAFGDDSREVFRVALPSANAATRATLRELTFDDVGATLI
                     ARSGVDLVAVHADGALQRVEGTACTEPLSPVGAAPGYAVFACRSGILMGIAERAVTGK
                     AK"
     gene            complement(1599..>2364)
                     /locus_tag="VFQ35_19150"
     CDS             complement(1599..>2364)
                     /locus_tag="VFQ35_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HET9932833.1"
                     /translation="IGRRLDPERSDRDFYRVTVPSGTPVVELTTRSLPNMALCTWVYA
                     SGSESPLGRYCAGAPGKDLRIEQLALPAGTYLLAVMQDRDAYTRAGAPPVYENVSDEY
                     TLRLAPRELDPNREVEPNDTPRNPTQVAPGAAVTGKLAFMRDVDVVCSTAPGKLRFVV
                     EETDGVMRPRESALQVKPLSGPDQEIPVRVHRGLQGVPRSPRDSASPWRGAPLQSDGS
                     PACLELSLVPNPWGPTPAPDIAPANETEYVVRVEIP"
BASE COUNT          375 a          762 c          851 g          376 t
ORIGIN      
        1 gcgggaaccg gattgtcttt ggatgtgtgg gtcggcttcg gcctttctcc agggttagtt
       61 ctggggccag ccatctccta ttggggcggc aacgacggcg atccgtccat cagtggcggt
      121 gcgagctctg gcgagtcggg cagcgcgctg ctcggcgctt tcgtcgacgc ttacccgaac
      181 ccgcgacgag gcgagcactt cggcggtgcg ttggcgctcg gcaccacgag cgcgaagctc
      241 tcgggtcggc ccgagatcaa ggactaccag ggcggtggcg tcggcttcct ggtgttcggc
      301 ggttacgatt tttggatcgc gagaacgtgg tcactcggtg ggctactgaa gctcagcggg
      361 ctcgcgactc gcggcaacag caccatcgat ggtcagaagg tggagcgcca agcgacgtcg
      421 tacgcggcgt ctattgccgt tactttgctt taccagtgac cgctctctcg gcgatgccca
      481 tgagtatgcc cgagcggcag gcgaacacgg cgtagcccgg cgcagccccc acgggagaca
      541 gtggctccgt gcacgcagtg ccctcgacgc gctgcaatgc gccgtccgca tgcaccgcca
      601 cgaggtcgac cccgctgcga gcgatgagcg tggccccgac gtcgtcgaag gtcagctcgc
      661 gcagcgtggc gcgcgtcgcc gcgttggccg atgggagcgc gacgcggaag acttcgcgcg
      721 agtcgtcgcc gaacgcgaat acgaagtcga ggttcgacat gaggcgcagg ccccgcggcg
      781 tgttctgtac gagcaacggc gagaactcga cgtcggtctc ggcgaaatga gtggtcggaa
      841 gttgggtcgc gacgttcagc tcgacgagcc gacggccgtc gacgagagcg aggacgctgt
      901 cgtcaccccg gcgcaccgcc gcatcgaccc gcgcgttgaa actcgcgact cgtcgccaac
      961 cgccgttgcg ggcgagctcg agcacgtttc cgttcgcaag cacgatgagc gaccggctcg
     1021 gcgctgggag tatcgcgcgt acgtcctgat cggcgcgagc ggtcgcgacc accccgagcg
     1081 aagcatcgag ctgcaaaacg cgccggccgg cggcaatgaa catcccgccg tcgggtgtcg
     1141 gggcgacgag gggcggcgcc tcgagcgcgg gcaacgggag gctgtgtcga cgaagcacct
     1201 ggcctcgagg cgaaaacgag atcgcttccc ccgcttgcgt gagcaccatg cgcgcgccat
     1261 ccccgaggac gatgaggctc gtggcggcgt tcgaggcgcc gagccgcgcc gtccagagta
     1321 ggcgcccccg cgcgtcgtac tcggcgacga ccggggttgc gagcccgagc accacggagc
     1381 cgtcgctggt tggcgttggc gcaaatggca ccgccgaagc cacgcggacg cgccatacga
     1441 cctcggggcg cagcggaagg ttaaagtcgc tgctgcgcgt cgaggtacga atgcgcgcca
     1501 acgagaactc gcggacgacg gtcgggtccc agcgttccgg cgatgcctgg gcggcagtgg
     1561 cggaggcgat gccgagcgtg agcagggcgg cctgcgacct acgggatttc gacgcgaacc
     1621 acatactcgg tctcgtttgc gggggcgatg tcgggggctg gcgtcggccc ccaaggattc
     1681 ggaaccaagc tgagctcgag gcaagccggg cttccatcgc tctggagcgg cgcgcctcgc
     1741 cacgggctcg ctgaatcccg cggagaccgc gggacgcctt gaagccctcg gtgcacgcgc
     1801 accggaatct cttggtcggg gccgctcagc ggcttcactt ggagagcgct ctcgcgcggt
     1861 cgcatcacgc cgtccgtctc ttcgacgacg aacctcagct tgcccggcgc cgtgctgcaa
     1921 acgacgtcga cgtctctcat gaaagcgagc ttgcccgtca ccgcggcgcc cggcgcgact
     1981 tgggtcgggt tccgcggagt atcgttcggc tcgacttcgc ggttcggatc gagctcgcgc
     2041 ggagcgagtc gcagcgtgta ctcgtcggag acgttttcgt agaccggggg cgcgcccgcg
     2101 cgcgtgtagg cgtcgcgatc ttgcatgacc gcgagcagat aggttcccgc tgggagggcg
     2161 agctgctcga tgcggagatc ctttccgggc gcgccggcgc agtaacgacc gaggggcgac
     2221 tccgagccgg aggcgtatac ccacgtgcag agtgccatgt tcggtaagct gcgagtggtg
     2281 agctcgacga ccggggtccc gctcggcacc gtgacacgat aaaagtcgcg atcgctgcgc
     2341 tccggatcga ggcgccgccc gatt
//
LOCUS       DASTKS010000916         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_147228, whole genome shotgun sequence.
ACCESSION   DASTKS010000916 DASTKS010000000
VERSION     DASTKS010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147228"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="apaG"
                     /locus_tag="VFQ35_19155"
     CDS             <1..444
                     /gene="apaG"
                     /locus_tag="VFQ35_19155"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET9932834.1"
                     /translation="LRLPSKPQRGTGTSMSQEEHQSTAVTDGIRISVRSRYLPEQSTP
                     AAGRYAFAYTVRIRNEGPQSARLRARHWIITDGAGATREVQGPGVVGEQPLLRPGEEF
                     EYTSGAVLQTPRGTMRGTYDMERPNGSAFDAVIAPFVLSLPYSLN"
     gene            complement(613..2088)
                     /locus_tag="VFQ35_19160"
     CDS             complement(613..2088)
                     /locus_tag="VFQ35_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET9932835.1"
                     /translation="MAPFAFDNTYARLPGRFYSRVAPTPVRDPRLVKLNQPLLRLLGS
                     TPEALQSLDPSRVFSGNEVPAGADPIALAYAGHQFGDFVPQLGDGRAIMLGEVVSGDG
                     VRRDVQLKGAGRTPFSRSGDGRAALGPVLREYVVSEAMAALGIPTTRALAAVTTGEFV
                     IRDTRLPGAVLTRVAASHLRVGTFEYFSVRRDRDALARLVDYALARHFPEPDDSSNPA
                     LRLLDRVVERQATLVARWLGVGFIHGVMNTDNTSISGETLDYGPCAFLDDYQPNKTFS
                     SIDHDGRYAFAKQPAMARFNSACLARTLLPLLADDHAVAVRLANERVERFSDLFMTAF
                     LGVFRSKLGLFGTASDDAALIDFVLERLAEQPIDYTNFFRHLCYAMADPSRDAAVATA
                     FRDSDGMLAWLGRWRARLASDGASVSARLDSMRSANPSLIPRNHRIQQAIDAAVQSSD
                     FKPFEELVEALSRPYDEQPRFAHLADPPRPDERVERTFCGT"
     gene            complement(2100..2519)
                     /locus_tag="VFQ35_19165"
     CDS             complement(2100..2519)
                     /locus_tag="VFQ35_19165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9932836.1"
                     /translation="MKCVVESAGSVASTATLGSHTMLFDQPRSVPGGEDSGPSPLDVM
                     VASVGACAHYFAAAFLLARRIAADGLRVVAIAEKERDPSHRISKLCLHVTLPASVPEQ
                     YTDPIEKAIRACPAFNTLVHPTNVAVELQREASISGP"
     gene            2790..>3418
                     /locus_tag="VFQ35_19170"
     CDS             2790..>3418
                     /locus_tag="VFQ35_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9932837.1"
                     /translation="MAKWFKDNAESIGRTPLVRLNRLQAQPRARLLAKVEGRNPAYSV
                     KCRLGAAMVWDAEKRGVLGPGKEIVEPTSGNTGIALAFVAAARGYSLTLTMPETMSLE
                     RRKLLTAYGARLVLTEGPKGMSGAVAEAERIAASDPNRYVLLQQFKNPANPAIHEATT
                     GPEIWEDTDGDVDIFVSGVGTGGTITGVSRYFKHVRQKPLFSVAVEPAA"
BASE COUNT          643 a         1080 c         1135 g          560 t
ORIGIN      
        1 ctgcgcttac cttctaaacc acaacgcgga accggaactt ccatgtcgca agaagagcac
       61 cagtcgaccg cagtcaccga cggcattcga atcagcgtac gctcccgcta cctgccagag
      121 caatcgacac ccgcggcggg acgctacgct tttgcgtaca cggtgcgcat tcgcaacgag
      181 gggccccagt ccgcgcgcct tcgagcgcgt cactggatca tcacggatgg cgcgggtgct
      241 acccgagaag tgcagggtcc gggcgtggtc ggtgagcagc ccctgctgcg tcctggtgaa
      301 gagttcgagt acacgagcgg agcagttttg cagacccctc gcggaacgat gcgcggtacg
      361 tacgatatgg aacgtccgaa cgggagcgcc ttcgacgcgg tcatcgcgcc gttcgtgctc
      421 tcgctcccct actcgctgaa ctgagccgtc tcacgaacgc tgcgcggagc actgcgacgc
      481 gagagtgcga gcagcccgtt gctacattgg cccgggttgg tgcgccgcag aacttggctc
      541 gcggcagggc ggagaactcg ggacacccta gtgcagcgcc ggcaatgtcg acgcatgctc
      601 acaggcgctg cactaggtac cgcagaaggt gcgttcgacc cgttcgtctg ggcgcggtgg
      661 atccgccaag tgagcgaacc gaggctgctc gtcgtatggg cgcgacagcg cctcgacgag
      721 ctcttcgaac ggcttgaagt cggagctttg caccgccgcg tcgatcgcct gctgaattcg
      781 atgattccgc ggaatcaacg aaggatttgc gcttcgcatc gagtcgaggc gcgcgctcac
      841 gcttgctccg tcggacgcca atcgggcgcg ccaacggccg agccaagcga gcatgccgtc
      901 gctgtctcga aacgccgttg cgacggcagc gtcgcggctc ggatctgcca tcgcgtaaca
      961 caggtgccga aagaagttgg tgtaatcgat gggctgttcg gcaagccgct cgagcacgaa
     1021 gtcgatgagc gctgcgtcgt ccgaagccgt accgaacaag ccgagcttcg accgaaacac
     1081 gccgaggaac gcggtcatga agaggtccga aaagcgctcg acgcgctcgt ttgccagtcg
     1141 aaccgccact gcgtggtcgt cggccaagag aggcagcagc gttctggcca ggcaggcgct
     1201 gttgaagcgc gccatcgccg gctgcttcgc gaaagcgtag cgaccgtcgt gatcgatgga
     1261 gctgaaggtc ttgtttggct ggtaatcgtc gaggaacgcg cacgggccgt agtccagcgt
     1321 ttcgcccgaa atcgacgtgt tgtccgtgtt catcacgccg tgaatgaagc cgacgcccag
     1381 ccagcgagcg accaacgtcg cctgacgctc cacgactcga tcgagcaaac gcaacgcagg
     1441 gttgctcgag tcgtcgggct caggaaagtg gcgcgccaaa gcgtaatcca cgaggcgcgc
     1501 caaggcgtcg cgatctctcc gcaccgagaa gtactcgaag gtgccgactc gcaggtggct
     1561 cgcggcgacg cgcgtgagaa cggcgccggg caggcgggtg tcgcgaatca cgaattcacc
     1621 cgtggtgacg gcagcgagcg cgcgggtagt cggaattccg agcgctgcca ttgcctcgct
     1681 caccacgtat tcgcgcaaca cggggccgag cgccgctcgt ccgtcgcccg agcgcgaaaa
     1741 tggagtacgt cccgcgccct tcagctgtac gtcccgacga acgccgtctc cgctcacgac
     1801 ctcgcccagc atgatcgccc gcccatcgcc gagctgggga acgaaatcgc cgaattgatg
     1861 ccccgcatag gcgagcgcga tggggtcggc gccggccggc acttcgttgc ccgaaaagac
     1921 gcgagacggg tcgaggctct gcagtgcctc cggagtcgaa ccgagcagcc gtaagagagg
     1981 ttgattgagc tttacgagcc ttgggtcgcg aaccggcgtg ggcgcgacgc gcgagtaaaa
     2041 gcggccgggg aggcgcgcgt aggtgttgtc gaaggcgaac ggagccatag cggggtgagc
     2101 tacggaccag agatgctcgc ctcgcgctga agctcgaccg cgacgttggt cggatgaacc
     2161 agcgtgttga acgcggggca ggcgcggatc gctttctcga ttgggtccgt gtactgctcg
     2221 ggcacgctgg ccggtaacgt cacgtgaagg cacagcttgc tgattcggtg agaggggtct
     2281 cgctcctttt cggcgatggc cacgacgcgc agcccgtccg cggctatccg gcgcgcgagc
     2341 agaaaagccg cggcaaagta gtgcgcgcac gcaccgaccg acgcgaccat cacgtcgagg
     2401 ggtgatgggc cgctgtcttc gcctccggga accgaacggg gttggtcgaa cagcatggtg
     2461 tgcgagccca gcgtggccgt gcttgcgacg ctgcccgcgg actctacgac gcacttcatg
     2521 cacgtagaac gaagcaccga atgtgccagc tgccgcgagc gcgaagcaag gagccttgcc
     2581 gccgccgcgc gcttcacgtc gcgtcacaaa gcgaacagcg cgtcgtaaat cgaacagctt
     2641 cgcggcgctc gttgctccct gattcgagtg ccattgttca acgaaaccgc tcactatggc
     2701 cgagcgcgag gatctcgtcg gtcgcgacga cgaactcgtc gcatggcacg cgcattgccc
     2761 cgctctcgtt cgagaaaggg cccgacccca tggcgaaatg gttcaaagac aacgctgagt
     2821 cgattggtcg cacgccgctc gttcggttga atcgcctaca ggcccagcca cgcgcgcgct
     2881 tgctcgcgaa ggtagagggc agaaatcctg cctactccgt gaagtgtcgc ctcggcgcgg
     2941 ccatggtgtg ggacgcggag aagcgcggcg ttctcggccc aggcaaggaa atcgtcgagc
     3001 ccacgagcgg caacaccggg atcgcgctcg ctttcgtcgc tgctgcgcgc gggtactcgc
     3061 tgaccttgac catgcccgag acgatgagcc tcgagcggcg gaagctgctc acggcgtacg
     3121 gagcgcgtct cgtgctcacc gagggcccca agggtatgag cggcgccgtg gcggaggcag
     3181 agcgcatcgc ggcctccgac ccgaatcgct acgtccttct gcagcagttc aagaacccgg
     3241 cgaacccagc aatccacgag gcgaccacgg gcccggaaat ttgggaagat accgacggcg
     3301 acgtcgatat cttcgtttcc ggtgtcggta ccggcggcac gattaccggc gtttctcggt
     3361 acttcaaaca cgttcggcag aagccgttgt tctcagtcgc ggtggagccc gcggcgag
//
LOCUS       DASTKS010000917         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1264205, whole genome shotgun sequence.
ACCESSION   DASTKS010000917 DASTKS010000000
VERSION     DASTKS010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264205"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFQ35_19175"
     CDS             complement(<1..755)
                     /locus_tag="VFQ35_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932838.1"
                     /translation="MSGRVRKTSAVEQQFLASGWELLGVAADEFPDPSALPSFGWVPA
                     RAPGTVASSLREAGVWDFNAGDPRLESKDWWWRVRFAGPPEKAADELALGLDGLATLA
                     EVWLNGVKVGSSASMFVAQRLPVELRAENELSIRFPALERALAARRPRPRWRVPMLEQ
                     QQLRWFRTSLLGHTPGWSPPCPAIGPYRAVFWERRVAPTLADIVIRPEVLAGRGVLKL
                     SCRLKDSVSAPFEAAVFVLERGGVTHRASAHREG"
     gene            complement(758..1009)
                     /locus_tag="VFQ35_19180"
     CDS             complement(758..1009)
                     /locus_tag="VFQ35_19180"
                     /inference="COORDINATES: protein motif:HMM:NF005480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9932839.1"
                     /translation="MANPHSEKIRNILKQHARLSTDVAALTDDSDLYQAGMTSHASVN
                     VMLALEAEFDIEFPDNMLKRGVFESISAMSEAVAKLTGG"
     gene            complement(1057..2040)
                     /locus_tag="VFQ35_19185"
     CDS             complement(1057..2040)
                     /locus_tag="VFQ35_19185"
                     /inference="COORDINATES: protein motif:HMM:NF020467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HET9932840.1"
                     /translation="MALSSLAVIPGLDATSYQRHALHAEERVWVEKNCYIDVWIELVH
                     ALGCDPVAMLGVVAALDFEGDQWTFFKPSHGEIHELYGLDVQELNVWRTLLEHVVEHV
                     PQGKLISTEADAFWLPDTSGTDYQRQHTKTTIVVNEIDVARERLGYFHNAGYYTLEGE
                     DFRRTFRVGAPHDPSFMPLFAETVQTRRLVRRTPVELRELARRFLVTHLLRRPVDNPV
                     ERFAARLAADLPLITERGLAHYHAWAFATIRQLGAAFELLAQHLLWLDDDAFESATAE
                     FSQIASGAKVLILKGARAVNSRRNVDLSSQFAELSNAWTNGMAALTSALSL"
     gene            complement(2031..2945)
                     /locus_tag="VFQ35_19190"
     CDS             complement(2031..2945)
                     /locus_tag="VFQ35_19190"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HET9932841.1"
                     /translation="MSEQAYDSKSFYDGLVQNRLIVPAGIQGAFGRSAVFEDVLEHVD
                     QLITQLSSQDGAERFTYPPVIDRRILEKVDYLDSFPQLAGCVHSFFGNERDARALSSA
                     IHAGEPWGQLMGQTDVVLNPAACYPVYPAMTGTLPQNGRLITMTNWVYRHEPSPEPTR
                     MQSFRVREFVRAGQPSDVVAWRDQWLERGLEMLKSLGLPARSDVASDPFFGRAGKMLM
                     EGQKAQRLKFEVLVPVISLENPTAVCSFNYHQDHFGQVFGIQSTNGEVAHTACLGFGL
                     ERVVMALFRTHGFDPQRWPKEVRDRLWH"
     gene            complement(2949..4109)
                     /locus_tag="VFQ35_19195"
     CDS             complement(2949..4109)
                     /locus_tag="VFQ35_19195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9932842.1"
                     /translation="MDSTELLAQTRLIAREVAAAHAADVDTNARFPSETFQALKKARL
                     LSVAVPKEYGGAGANMRTLTEMCTILAQSCAASGMILAMHHIQVACLARHGLPSQYFA
                     DYLREAVERQLLIASITSEVGVWGDTRSSICALEREGERFRLVKDATTVSYGAHADDL
                     LVTCRRNPDSPPGDQILVLVRQGDRTLTQTTNWDTLGMRGTCSPGFKMVASGKIEQVV
                     PGSFADASAQTMVSYSHILWSGVWLGIATDAYSRAAAYVRSEARKKPGTVPNSAHRLA
                     ELAVALQAMRNNTVAQATGFDEIMQMPNGMDELLTIGWALRMNNIKVGSSEAAPRIVH
                     DALQIVGIGGYKNDGKYTVGRNYRDALSAALMISNDRIFAKTASMLLVFKDE"
     gene            4319..5089
                     /locus_tag="VFQ35_19200"
     CDS             4319..5089
                     /locus_tag="VFQ35_19200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9932843.1"
                     /translation="MALATEQRTVALMYHAVGVTSGAGVDPHYAVGTATFERQMQICL
                     AQGGGVLSALQWLRGERGIIVTFDDGHVTNYLHAFPALKSLGGSADFFVNPAQVGTEG
                     FATWAELREMSDGGMSIQSHGLDHRHYLTELSPADLRDELRRARLEIEEHMGKPVTLL
                     APPGGRMPPRLEDVALEVGYTHVLTSKPGTVRRGTRMMCRLAVTRTLEEKTLDSWLRG
                     GIELRRTQLRQAGLDAAKRLLGDRRYEAVRNSLLRARG"
     gene            complement(5112..5537)
                     /locus_tag="VFQ35_19205"
     CDS             complement(5112..5537)
                     /locus_tag="VFQ35_19205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9932844.1"
                     /translation="MQVVKLQVPARLDYRELATRTIALVCKIATSKGDTEDKREELTN
                     ELVSAVGEAFNNVVLHGYGRTRNGDLSIITEFDTKRIVVELHDYGASFDPGAVALPDL
                     GRLPESGLGLYIIRSFADEVEYSAGPPNVLRLVKFLPVS"
     gene            5853..>6508
                     /locus_tag="VFQ35_19210"
     CDS             5853..>6508
                     /locus_tag="VFQ35_19210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9932845.1"
                     /translation="MIDLVASLAGLTLTVPFYPLIAIAIKLDSPGPIFYKQRRAGAMK
                     RGKAGEAPEFSDFWIVKFRTMRTDAEKNTGAVIASKGDPRITKIGRFLRKTRIDELPQ
                     FLNVLRGDMSLVGPRPERPELLADLALAIPFFEERLREAKPGLTGLAQVTLGYTGAMP
                     DDSELAPFRDILQNPFDLPEADGALADDMRIKLLYDLAYCAALEKFSTYLAMELRVML
                     "
BASE COUNT         1161 a         2128 c         2087 g         1132 t
ORIGIN      
        1 ccttcgcgat gagccgaagc ccggtgggtc acgccgccgc gctccaaaac gaacaccgcc
       61 gcttcgaacg gcgcgctcac ggagtctttc aagcggcagc tcagcttcaa gacgccgcgt
      121 ccggcgagaa cctcggggcg aatcacgatg tcggcgagcg tcggcgcgac acggcgctcc
      181 cagaacacgg cgcgataggg accaatcgcc ggacaaggag gagaccaacc cggcgtgtga
      241 ccgagcagcg aggtgcgaaa ccagcgcagc tgctgctgtt cgagcatcgg aacgcgccat
      301 cgagggcgcg ggcggcgcgc ggcgagggcg cgctcgagcg ctggaaagcg aatcgaaagc
      361 tcgttttcgg cgcggagctc gaccggcagc cgctgggcca cgaacatgct cgcgctgctt
      421 ccgaccttca cgccgttgag ccacacctcc gcgagcgtgg cgaggccgtc cagccccagc
      481 gcgagctcgt cagccgcctt ttcgggcgga ccggcgaagc gcacgcgcca ccaccaatcc
      541 ttcgactcga gccgcggatc gccggcgttg aagtcccaaa cgccggcttc gcgcaggcta
      601 ctcgcgacgg tgccgggggc gcgcgccggc acccaaccga agctcggcag cgcgctcggg
      661 tccgggaact cgtccgcggc gacgccgaga agctcccaac cgctggcgag gaactgctgc
      721 tcgacggcgc tcgttttacg gacgcgcccg gacacgctca cccgcccgtt agcttcgcga
      781 ctgcttcgga catggcgctg atgctctcga acacgccgcg cttcagcatg ttgtcgggga
      841 actcgatgtc gaactcggct tcgagcgcga gcatcacgtt cacgctggcg tgcgacgtca
      901 tccccgcttg gtagaggtcg gagtcgtcgg tgagcgcggc cacgtcggtg ctgagccgag
      961 cgtgctgctt caaaatgttc cggatctttt cgctgtgagg gttggccatg aatcgagttc
     1021 gtttcggtgg tgagcgtcgc gagaccgcgc ggcctctcag agtgacagcg cgctcgtcag
     1081 cgccgccatg ccgttcgtcc aggcgttcga aagctccgca aattgacttg ataggtcgac
     1141 gttccggcgc gaattgacgg cgcgcgcgcc cttgaggatg agcaccttgg cgccgctcgc
     1201 gatctggctg aactccgccg tcgccgactc gaacgcgtcg tcatcgagcc agagcaagtg
     1261 ctgggcgagg agctcgaacg cggcgccgag ttgacggatc gtcgcgaagg cccaggcgtg
     1321 atagtgcgcg agcccgcgct cggtgatgag cggcagatcc gccgcgaggc gggccgcgaa
     1381 gcgctcgacc gggttgtcga cgggccgccg caggaggtga gtgacgagga aacgacgcgc
     1441 gagctcacgc agctcgaccg gcgtgcggcg gacgagccga cgggtttgca ccgtctccgc
     1501 gaacaggggc atgaagctcg ggtcgtgcgg cgcgccgacg cggaaggtgc gccgaaaatc
     1561 ttcaccctcg agggtgtaat agccagcgtt gtgaaagtac ccgagccgct cgcgcgccac
     1621 gtcgatctcg ttgacgacga tcgtcgtctt ggtgtgctgg cgctggtagt cggtcccgct
     1681 cgtgtccggc agccaaaatg cgtctgcctc ggtcgagatc agctttcctt gcggcacgtg
     1741 ctcgacgacg tgctcgagca gcgtgcgcca cacgttcagc tcttgcacgt cgagcccata
     1801 gagctcgtgg atctctccgt gcgatggctt gaagaaggtc cactggtcgc cctcgaagtc
     1861 gagcgcagcc acgaccccca gcatcgcgac cggatcgcag ccgagcgcgt gcacgagctc
     1921 gatccacacg tcgatgtagc agttcttctc gacccaaacg cgctcttcgg cgtggagggc
     1981 gtgccgctgg tacgacgtcg cgtccagccc cggaatcacc gcgagcgaac tcaatgccac
     2041 aacctgtcgc gcacttcctt cggccagcgc tgcggatcga agccgtgcgt cctgaacagc
     2101 gccatcacga cgcgctcgag gccgaagccc aagcacgcgg tgtgagcgac ttcaccgttc
     2161 gtcgattgga taccgaagac ctgcccaaag tgatcttggt gatagttgaa cgagcacacc
     2221 gccgtcgggt tctcgagcga aatcaccggg accagcacct cgaacttcag gcgctgcgcc
     2281 ttctgtcctt ccatcaacat cttgccggca cggccgaaga acggatccga cgcgacgtcg
     2341 gagcgcgcgg ggagcccgag cgacttgagc atctcgagcc cgcgctcgag ccattgatcg
     2401 cgccaggcga ccacgtccga gggttgcccg gcacgcacga actcgcgcac gcggaaggac
     2461 tgcatgcgag tgggctccgg cgacggctcg tgccgataga cccagttggt catggtgatg
     2521 aggcggccgt tctgcgggag cgtccccgtc atcgcggggt agaccggata gcaagcggcc
     2581 gggttcaaca cgacgtcggt ctgccccatg agctgacccc acggctcgcc cgcgtgaatg
     2641 gcgctcgaca gagcgcgcgc gtcgcgctcg tttccgaaga agctgtgtac gcagcctgcg
     2701 agctgcggaa atgaatcgag gtagtcgacc ttctccaaaa tgcgccgatc gatcaccggc
     2761 ggataggtga aacgctcggc tccgtcttgg ctcgagagct gcgtgatcaa ctgatcgacg
     2821 tgctcgagca cgtcctcgaa cacggcgctc cgcccgaacg cgccctggat acccgccggg
     2881 acgatcagac gattttggac cagcccgtcg taaaaagact tcgaatcgta ggcttgctcg
     2941 gacatcgttc actcatcctt gaagacgagg agcatcgacg ccgtcttggc gaagatgcgg
     3001 tcgttgctga tcatcaacgc ggcggagagc gcgtcacggt agttgcgccc gacggtgtac
     3061 ttgccgtcgt tcttgtagcc accgatcccg acgatctgca gagcgtcgtg gacgatgcgc
     3121 ggcgcggctt ccgacgagcc gaccttgatg ttgttcatgc ggagcgccca accgatggtg
     3181 agcagctcgt ccatgccgtt cggcatctgc atgatctcgt cgaagccggt cgcctgggca
     3241 accgtgttgt tgcgcatggc ttgcagcgcg accgcgagct cggccaaccg gtgcgcggag
     3301 ttcggtaccg tacccggctt cttgcgggcc tccgaccgca cgtaggcggc cgctcgcgag
     3361 taggcgtcgg tggcgatgcc caaccaaacg cccgaccaca agatgtgcga gtaggaaacc
     3421 atggtctgcg ccgaagcgtc cgcgaaggag cctggcacca cctgctcgat cttcccgctc
     3481 gcgaccatct tgaagccggg gctgcaggtg ccgcgcatcc cgagcgtgtc ccagttggtc
     3541 gtctgcgtga gcgtgcggtc gccttgacga accaagacga ggatctgatc tcccgggggc
     3601 gagtcggggt tgcggcggca cgtgacgagc aggtcgtccg cgtgcgcgcc atacgagacg
     3661 gtagtcgcgt ccttcaccag gcggaagcgc tcgccctcgc gctccaaggc gcaaatgctg
     3721 gagcgggtgt caccccaaac tccaacctcc gaagtgatgg aggcgatcag gagctgccgc
     3781 tcgaccgcct cgcgcaggta gtcggcgaag tactgcgaag gcaagccgtg gcgcgccaga
     3841 caggcgacct ggatgtgatg catcgcgagg atcatcccgc tcgcagcgca ggactgcgcg
     3901 aggatcgtgc acatctcggt cagcgtgcgc atgtttgcgc ctgcgccgcc gtactccttc
     3961 ggcaccgcca ccgagagcag gcgagccttc ttcaaggctt ggaacgtctc gctggggaag
     4021 cgagcattcg tgtcgacgtc cgcggcgtgt gcggcggcga cttcacgagc aatcagacgc
     4081 gtttgagcaa gtagttcggt cgagtccacg cttcaccacc agaggtcgcg ctcggtaatc
     4141 gagcccgagt gaacggacag accttctttc gtagctcgtt tctcttccat gcgtcaagct
     4201 cgcgcgctca cgcgatcggt cacaccggat agtaagtttt ccgcattgcc ttcgcgttac
     4261 gcactcggcg cgcgagggcg tcgaagaggt ccttcggcgt gctagcgttc gtcagtcaat
     4321 ggcgctcgcg accgaacaac gaaccgtcgc tctcatgtac cacgcggtcg gcgtcaccag
     4381 cggcgctggc gtggatcctc actacgcggt cggaaccgca actttcgagc ggcaaatgca
     4441 gatttgcctc gcgcagggcg gtggcgttct cagcgcgctc caatggctac gcggtgagcg
     4501 cggcatcatc gtcacgttcg acgacggtca cgtgacgaat tacctgcacg catttccagc
     4561 tttgaagagc ctgggcggct cggcggactt cttcgtgaac cctgcgcaag tcggcaccga
     4621 aggtttcgca acgtgggccg agctccgcga aatgtccgac ggcggcatgt cgatccagtc
     4681 gcacgggctc gatcatcgcc actacttgac cgagctctcg cccgccgact tgcgcgacga
     4741 attgcggcgc gcgcgcctcg agatcgaaga gcacatgggc aagccggtaa cgctgctcgc
     4801 gccgcccgga ggccgcatgc cgcctcgctt ggaggacgtt gctctcgagg tcggctacac
     4861 ccacgtgctc acgtcgaagc caggcaccgt gcggcgcggt acccggatga tgtgccgtct
     4921 cgcggtgacg cgaacgctcg aggaaaagac gctcgattcg tggcttcgcg gcggcatcga
     4981 gctgcgccga acgcagcttc gacaagccgg cctcgacgcg gccaagcggc tgctcggcga
     5041 tcgccgctac gaagcggtgc ggaactcgct tctgcgcgcg cgcggctgac accgcacacg
     5101 gcaattactt ttcagctcac cggcaagaac tttacaagtc gcagcacgtt gggcggaccg
     5161 gccgagtact cgacctcgtc cgcgaaggac cgaatgatgt aaagcccgag ccccgactcc
     5221 ggcaaacgtc cgaggtccgg cagcgcgacc gcaccgggat cgaagctcgc gccgtagtcg
     5281 tgtagctcga cgacgatgcg cttcgtgtcg aactcagtga tgatgctgag gtcgccgttc
     5341 cgcgttcgcc cgtagccgtg cagaacgacg ttgttgaacg cttcgccgac cgcagatacg
     5401 agctcgttcg tcagctcctc ccgcttgtcc tccgtgtcgc ccttgctcgt tgcaatcttg
     5461 caaacgaggg cgatcgtgcg agtcgcaagc tcgcgatagt cgagcctggc gggtacttgg
     5521 agcttgacta cttgcaaggc agcctgcctg tcatcaatgc tacgcggtgg gagaccttgg
     5581 cgctacggtc gcgaagacaa acctgcggtc gcgatcgcaa gccaagttcc tgacggttgc
     5641 agcccgacgg gaactcgccc ttccctccag ggatcgaatt gcctcagcca caagcgagat
     5701 cacttctcac tctccctagc cctcgtagcg ctcaaaggca aatttgctca tttgccacgg
     5761 tacccggtgg ggccggtacg gggcttgaat cgcacaacct cgctcagtag attcgtacat
     5821 taatgcccct aaccgttcga gtcacaaagc ggatgatcga cctcgtcgct tccttggcgg
     5881 gtctcacgct taccgtgcct ttttatccgc tgattgcgat cgcaatcaag ttggactcac
     5941 cgggccccat cttctacaag cagcgccgcg ctggcgcgat gaagcgcgga aaagcggggg
     6001 aggcgccgga gttttcggac ttttggatcg tcaagtttcg gacgatgcgc acggacgccg
     6061 aaaagaacac cggcgccgta atcgcgtcca agggcgaccc tcgcatcacg aagattgggc
     6121 gcttcctgcg aaagacgcgc atcgacgagc tgccgcagtt cctcaacgtg ctgcgcggcg
     6181 acatgagcct cgtggggccg cgccccgaac ggcccgagct cctcgcagat ctcgcgctcg
     6241 cgatcccgtt cttcgaggag cggctgcgcg aagccaaacc gggtctcacc gggctcgctc
     6301 aagtgacgct cggctacacc ggcgcaatgc ccgacgacag cgagctcgcg ccgtttcggg
     6361 acattctcca aaaccccttc gacctcccgg aggcggacgg tgcgctcgcg gacgacatga
     6421 ggatcaagct cctgtacgat cttgcgtact gcgccgcgct cgaaaagttc tcgacctatc
     6481 tggcgatgga gctccgcgtg atgttgaa
//
LOCUS       DASTKS010000918         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_369841, whole genome shotgun sequence.
ACCESSION   DASTKS010000918 DASTKS010000000
VERSION     DASTKS010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369841"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFQ35_19215"
     CDS             complement(<1..295)
                     /locus_tag="VFQ35_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932846.1"
                     /translation="MTISHPLLAPLRALHETIVEGVVAACARKSVEELSTVAADDAGD
                     TIYAVDRVSEELLVRELARDADNLGGIVLIAEGVADGTLTLPSGRDEGRCRYRI"
     gene            complement(292..1323)
                     /gene="galT"
                     /locus_tag="VFQ35_19220"
     CDS             complement(292..1323)
                     /gene="galT"
                     /locus_tag="VFQ35_19220"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9932847.1"
                     /translation="MTWEERWHPLRREWVIVAAHRQNRPWSGGTVGPRAERRPSYVAD
                     CYLCPGNERVSGAHNPAYRDVFVFDNDHPAVSELAPREPEPRPSFYRAQPARGVARVV
                     CYTPRHDASLAELEPAGLMALLREWQRQYVELGRRPEVAHVLVFENRGEVVGVSNPHP
                     HCQIYATNFVFPTIRTEVEASLEHRAETGRNLFQDIISAELEDGRRVITQNDGALAFV
                     PYFARYAYETYVAPRRSYPSIADVPPAELAQFAEVLRDVLVRFDNLWQMPFPYVMTLH
                     QAPTDGGDYQSYHFHVQFQPPLRKPNLLKYLAGPEIGGGGFLSDTSPENKAEDLRSAS
                     GPHYRSVTP"
     gene            1410..3212
                     /locus_tag="VFQ35_19225"
     CDS             1410..3212
                     /locus_tag="VFQ35_19225"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9932848.1"
                     /translation="MPDAPELLDCRHAAICAGCPLAFVAYARQLVGKRDRLEKALQAY
                     GVLTALTVPNTVPASPNTRYRVRVKLVVEGSALGLFHKDGHAVVDIPDCRVMTPKLGA
                     AVAALRSMLPLEFPLLALDAREVDAGVLATLIVPPDVSRARVEGAALDLMHRCGSVVG
                     VALARRAPKSAQVLGSAPEPLVGAFAAPHRLADSMPYHIATPGGFVQAHAGQAAKLYE
                     RIGQVLERKLGGLRGRRILELYAGSGALALHLAQASARVVAADSFAPSLSQAREAAAQ
                     QGLDMEIREATAEKAVETREPFDAVLVDPPRKGLSASVRSGIAVLRPSVLVYVSCEPR
                     TLARDLAALTVLGLRPESVEPWDMIPQSDAVEALAVLTPAPPPPPRVLYEDERLIAVD
                     KPAQLPTTPQGEHELSLLDAVRKLPQASEAVPVHRLDLGTSGVCLFARRPEYAALLGE
                     ALAQGQKEYLGLARGITHKRGRIERALNDGTGVRDAVTRYERTQVVGTHSLVRAMPET
                     GRRHQIRRHFAHIGHPLLGDARYGNPASNRHFEERHGLDRPFLHLERITLPAMPVLRS
                     ELAPELAAVLESIEETNARRPRSGPGQKAPPRGE"
     gene            complement(3174..3731)
                     /locus_tag="VFQ35_19230"
     CDS             complement(3174..3731)
                     /locus_tag="VFQ35_19230"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9932849.1"
                     /translation="MTSPKPWQVLTETTLLDRRWLRLREQRVKLSNGHEIEQFHVIDG
                     PDWAAVVALTPTDELVMVRQYRHGASALSLELPAGVIEPTESPERAAERELLEETGYV
                     AESWRSLVVVCPEPARHTNRAHFFVALGARRVAEAAPEPSEELQIELHPVSRLMELIS
                     AGDICHGVHIGVLLLAARRGFLPGS"
     gene            3749..5317
                     /locus_tag="VFQ35_19235"
     CDS             3749..5317
                     /locus_tag="VFQ35_19235"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9932850.1"
                     /translation="MSPVDVIVRRLEDPGYTPARADLGELFEALPALPEESQKRAHRV
                     LLRAGWPAAEAAVARLNSAAQELAEELVAFLGRYAAKESDPRLLSPLLVVLATGSARA
                     RRLAASALGKLGDAQAENALLDALASAPLDLARAIVEALGKLGGERALASLRARRDSD
                     PEIVRRREQAELLIERRLGRGEQSEIELDARLPAASAIHIECRRGLVPLLVDELRAFG
                     ATPHGATSVRLTHAGTLRDLLRARTALSFGIYVGNSTAEPERIAALVTSEPVVAAVSA
                     WTRGRPRFRIDWVSAGHQRAASWAVAREVSRRSAALVNDPRAATWTLEIGAPPTGELR
                     FVPALEPDPRFAYRQRDVPAASHPTIAAALARMAGAQADDVVWDPFVGSGLELVERAL
                     LGPYRKLIGSDLDARALEAARANLNAANARDFELALGDMRTLAPRAVTLVISNPPMGR
                     RVARDGSLVGLLEAFVAQIARTLSPRGRAVFLSPLPARTEAWSKEKGLSFSRGPSVDL
                     GGFEAEIQTLRQTR"
     gene            5582..>5903
                     /locus_tag="VFQ35_19240"
     CDS             5582..>5903
                     /locus_tag="VFQ35_19240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9932851.1"
                     /translation="MRASVRHLLGIGALALTACSSDVQEAADGKIEQPIVRAAAQGGK
                     DQVVLLYMRTVSNGQFRSRTCTGTYIGPRLVLTAAHCMSNVVSNLVYAYWGDNFDSEF
                     AAIPQ"
BASE COUNT          955 a         2017 c         1954 g          977 t
ORIGIN      
        1 tgatgcggta tcggcagcgg ccctcgtctc gaccgctcgg cagcgtgaga gtgccgtcgg
       61 cgactccctc cgcaatgagg acgatgccac ccagattgtc cgcgtctcgc gcgagctcgc
      121 gcaccaagag ctcttcactg actcggtcga ccgcgtagat ggtgtctccc gcgtcgtccg
      181 cagcgaccgt cgacagctct tcgacgctct ttcgtgcgca cgcggcgacg actccctcga
      241 cgatggtctc gtggagcgcc cggagcggcg cgagcagcgg atgcgaaatc gtcatggcgt
      301 gaccgagcga tagtgaggtc ccgaggcgga gcgcaagtcc tcggccttgt tctctggtga
      361 ggtgtcgctc agaaaccctc cgcccccgat ttcggggccc gccaagtact tgaggaggtt
      421 cggcttgcgg agcggaggct gaaactgaac gtgaaagtgg tagctctggt agtcaccgcc
      481 gtcggtcggc gcctggtgca gcgtcatcac gtacggaaat ggcatctgcc aaaggttgtc
      541 gaagcgaacg agcacgtcgc ggagcacctc tgcaaactgc gctagctccg cgggcggtac
      601 atcggcgatg ctcgggtagc tgcggcgcgg agcgacgtag gtctcgtagg cgtatcgcgc
      661 gaagtacgga acgaacgcga gtgccccatc gttttgcgtg atcacccggc gcccgtcttc
      721 gagctcggca gagatgatgt cttggaacaa gttccgcccg gtctcggcgc ggtgctcgag
      781 tgaagcctcg acctcggtgc ggatggtcgg gaagacgaaa ttcgtcgcgt agatttggca
      841 atgcgggtgc ggattggaga cgccgaccac ttcaccgcgg ttttcgaaga cgagcacatg
      901 agcaacttct gggcggcggc cgagctcgac gtactggcgc tgccactcac gcagcagcgc
      961 catgagccct gccggctcga gctctgcgag cgaggcgtcg tggcggggcg tgtagcaaac
     1021 gacgcgcgcg accccgcgtg caggttgcgc gcggtaaaac gagggtcgag gttcgggttc
     1081 tcggggtgcg agctcgctca cggccgggtg atcgttgtcg aacacgaaga cgtcgcggta
     1141 ggccggattg tgcgccccgc tgacgcgctc gttgcccggg caaaggtaac aatcggcgac
     1201 gtagctcggt cggcgttccg cgcgtggccc gaccgtcccc cccgaccagg gccggttttg
     1261 gcggtgagcc gcgacgatga cccactctcg gcgcaggggg tgccagcgtt cttcccaggt
     1321 catgtgtctc gcgccggtat gacatggccg gcccggcgat agaagcgcct ccagtcgaag
     1381 agcggcgccc gcttgctaag ctcgcgcccg tgcccgacgc acccgaactc ctcgattgcc
     1441 gccacgcagc catctgcgcc gggtgcccgc ttgccttcgt cgcctatgcc cgacagctgg
     1501 tcgggaagcg cgaccgcctc gaaaaggcac tccaggcgta cggcgtgctg acggcgctga
     1561 cggtaccgaa cacggtgccc gccagcccga acactcgata tcgcgtgcga gtgaagctcg
     1621 tcgtcgaagg cagcgcactc ggcttgtttc acaaggacgg tcacgccgtc gtcgatatcc
     1681 ccgactgccg tgtgatgacg ccgaagctcg gcgccgcggt cgccgcgtta cgcagcatgt
     1741 tgcccctcga gtttccgttg ctcgcactcg acgcgcgcga ggtggacgcg ggcgtgctcg
     1801 cgacgctcat cgtccctccc gatgtgagcc gtgctcgcgt ggagggcgcc gccctcgact
     1861 tgatgcatcg atgcgggagc gtggtcgggg tagcgctcgc gcgccgcgcg ccgaagtcgg
     1921 cgcaggtgct cggctccgcg ccggagccac tcgtcggcgc attcgccgcg ccccaccgac
     1981 tggcagactc gatgccgtac cacatagcta cgccaggcgg cttcgtgcag gctcacgcag
     2041 gccaagccgc gaagctttac gagcgcatcg ggcaggttct cgaacgaaag ctcggtggcc
     2101 tccggggccg ccggatcctc gagctctacg cggggtcggg cgcgctggcg ctgcacttgg
     2161 cgcaagcttc tgcgcgcgtc gtggctgccg acagcttcgc gccgagcctt tcccaagcgc
     2221 gtgaggcggc tgcccagcaa gggctcgaca tggagatccg cgaggccact gcggagaagg
     2281 cggtagaaac tcgagaaccg ttcgatgccg tgttggtcga tccgccccgc aaggggctgt
     2341 cggcgagcgt gcgctccggg atcgccgtcc ttcggccgag cgtgctcgtc tacgtctcgt
     2401 gcgagccgcg gacgctcgcg cgcgacctcg ccgcgctgac ggtcctcggt ttgcgaccag
     2461 agtcggtcga accctgggac atgatcccgc agtcggatgc ggtcgaagcc ctcgcggtgc
     2521 tcacgcccgc cccgccgccg cctccccgcg ttctctacga agacgagagg ctcatcgcgg
     2581 tggacaaacc cgcgcagctt ccgaccacgc cgcagggcga gcacgagctc tcgctgctcg
     2641 acgccgtccg caagttaccg caggcgagcg aggccgtgcc ggtgcaccgc ctcgatctcg
     2701 gcacgagcgg tgtttgcttg ttcgcccgac gaccggagta cgcggctctg ctcggcgaag
     2761 cgctggctca agggcaaaag gagtatctcg gactcgctcg cggcatcact cacaaacgcg
     2821 gccgcatcga gcgagcgctg aacgacggaa ccggcgttcg cgacgccgtg acgcgctacg
     2881 agcgcactca ggtggtgggc actcattcgc tcgtgcgggc catgcccgaa accggtcgac
     2941 gccatcaaat tcgccggcat tttgcccaca tcgggcatcc cctgctcggc gacgcacgct
     3001 acggaaaccc cgccagcaac cgccatttcg aggagcggca cggcctcgat cggcccttct
     3061 tgcacctcga gcgaatcacg ctcccggcca tgcccgtgtt gcgctccgag ctcgccccgg
     3121 agctcgcggc ggtgctcgaa agcatcgagg agacgaacgc gagaagacct cgctcaggac
     3181 ccgggcagaa agccccgccg cgcggcgagt aagagcacac cgatatgcac gccgtgacaa
     3241 atgtcgccag ccgatatgag ctccatcagc ctgctgacgg ggtggagctc gatctgcagc
     3301 tcttcgctcg gctcgggcgc cgcttccgcg acgcgacgcg cgccgagggc cacgaagaag
     3361 tgggcgcgat tggtgtggcg cgccggctcc ggacagacca cgacgagcga gcgccacgac
     3421 tcggcgacgt agcccgtctc ttcgagcagc tcgcgctcgg ccgcgcgctc cggcgactcc
     3481 gtcggctcga tgactccggc cggaagctcc aggctgagcg cgctcgcacc gtgccggtac
     3541 tgccgcacca tcacgagttc gtcggtcggg gtgagcgcca ccaccgccgc ccaatccgga
     3601 ccgtcgatga cgtgaaactg ctctatttcg tggccgttcg agagcttcac ccgctgttcg
     3661 cgcagccgca gccaccgccg gtcgagcagc gtcgtttcgg tgagcacctg ccaaggcttc
     3721 ggcgacgtca tgcgcggggt cttagcagat gagtcccgtc gacgtcatcg ttcgccgcct
     3781 cgaagacccc ggctacacac cggcgcgcgc cgacctcggc gaactcttcg aagcgctacc
     3841 ggcgctgccg gaagagtcgc agaagcgtgc gcatcgcgtg ctgctccgcg ccgggtggcc
     3901 agccgccgaa gcggcggtgg ctcgtctgaa cagcgccgcg caagagctcg cggaggaact
     3961 cgtcgctttc ctcggacgct acgcggcgaa agagagcgac ccgcggctgc tctctccgct
     4021 gctcgtcgtc ctcgcaaccg gctcggcccg cgctcggcga ctcgcggcct cggcgctggg
     4081 caagctcggc gacgcgcaag ccgagaacgc gctgctcgac gcgttggcga gcgcaccgct
     4141 cgatctggcg cgcgcgatcg tcgaggcgct gggcaagctc ggcggagaac gcgcgctcgc
     4201 gtcgctcaga gcgcggcgcg atagcgaccc cgagatcgtg cggcggcggg agcaggccga
     4261 gctcctcatc gagcggcgcc tcggcagagg tgaacagtcc gaaatcgaac tcgacgcgcg
     4321 cttacccgca gcgagcgcga tccacatcga gtgtcgccgc gggctcgtcc ccctgctggt
     4381 cgacgagctc cgagcgttcg gcgcgacgcc gcacggagcg acgagcgtac ggttgaccca
     4441 cgcgggcacg ctgcgagacc tgcttcgtgc ccgcaccgcg ctgtctttcg gtatttacgt
     4501 gggcaattcc accgccgaac cggaacgcat cgcggccctc gtcacgagcg agccggtcgt
     4561 cgcggccgtc agcgcctgga cgcgcgggcg cccccggttt cgcatcgact gggtttcagc
     4621 ggggcaccag cgcgccgcca gttgggccgt cgcgcgtgaa gtttcgcggc gcagcgccgc
     4681 cctcgtgaac gatccgcggg cggcgacttg gaccctcgag atcggcgcgc ccccaaccgg
     4741 cgagctgcgc ttcgttcccg cgctcgagcc cgaccctcgc ttcgcctatc gccagcgtga
     4801 cgtgccggcg gcgtcgcacc ccaccatcgc cgccgcgctc gcgcgtatgg caggcgccca
     4861 ggcggacgac gtggtttggg atccgttcgt ggggagcgga ctcgagctcg tcgagcgcgc
     4921 gttactcggc ccctatcgca aactcatagg ctccgatctc gacgcgcggg cgctcgaggc
     4981 ggctcgggcg aacctgaacg ccgcaaacgc gcgcgatttc gagctcgcgc tcggagacat
     5041 gcgcactctc gcgccgcgcg cagtgacact cgtcatctcg aatcctccaa tgggccgccg
     5101 cgtggcgcgc gacggttcac tcgtcggcct gctcgaagcc ttcgtcgcgc aaattgcgcg
     5161 cacgctgagc ccgcggggtc gcgccgtctt tctctctcca ctgccagccc gcaccgaagc
     5221 gtggagcaaa gaaaagggac tttccttcag ccgcggcccg agcgtggatc tcggcggttt
     5281 cgaggctgaa attcagactc tgcggcaaac tcgctgagtg cattcgcgct cccgcgacct
     5341 cgcaattccg tccgtacgcg cagcgtgacg agcgctcgca tagtaacaag ttgtgcaatc
     5401 acccctgcgt actatcgcgc aggttttccg ctagaatgtg cgcgaacttt ctctgcgcaa
     5461 cttgcgcaaa agaattgaca aggatgaacc tacatcgaca gatcgattgt ctcgctgtta
     5521 acttcaagta acgatgcttg cggagttccc gcaaagcaga aacaacgagg tttcaggtat
     5581 catgcgcgct agcgttcggc atttactggg catcggagcc ctcgcactta ccgcgtgttc
     5641 ttcggatgtt caggaagcgg cggacggcaa aatcgaacaa ccgatcgtca gggccgcggc
     5701 gcagggcggc aaagatcagg tggttttgct ttacatgcga acggtgagca atgggcagtt
     5761 caggtcacgt acctgcacag gcacctacat cggcccccgc ctggttttga ccgcggcgca
     5821 ctgcatgtcg aacgtcgttt cgaacctagt ctacgcgtac tggggcgata acttcgacag
     5881 cgagttcgcg gcgatcccgc aat
//
LOCUS       DASTKS010000919         8208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1488360, whole genome shotgun sequence.
ACCESSION   DASTKS010000919 DASTKS010000000
VERSION     DASTKS010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488360"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFQ35_19245"
     CDS             complement(<1..1197)
                     /locus_tag="VFQ35_19245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9932852.1"
                     /translation="MKGLNLHGTRRARMFEWHRIALFACAASLGCSPSGSEPSSAHEN
                     TGRDRGGTSGASSGGKGAGGSTGIALGGAVNSAGEASATGGCPEPSACRVGCGNGRLE
                     LGLGETCDDGNDRAGDGCSADCRIVETNFVCPEPGRPCLNLVRCGDGKLAGGETCDDG
                     NVTGGDGCSAGCEIEPGFDCISEGARCTPHCGDGALKGDEQCDAPNGGKGCGVGCRLE
                     PGFVCAPPQGGPAMCHATVCGDRLREGAEACDDGNALDGDGCSAACALEPECSGGACV
                     PRCGDQLRISPEECDDGNNVDGDGCDRACRIENGFSCAENAASLPNSLNLTVTYRDFV
                     SFPIADLPRHPDFESAFAADERTPGLVRAELGADGKPQLEGRCSSEQPSTSSDRSICP
                     YGQMLTT"
     gene            complement(1446..2321)
                     /locus_tag="VFQ35_19250"
     CDS             complement(1446..2321)
                     /locus_tag="VFQ35_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932853.1"
                     /translation="MGLSRCLFGASVSLALLFGACSEPSLRVGYDDLSAGGISTVGGS
                     AGSANSGSGGVAGAPACKVTRCESNKAPYQCGDCMDNDGDGLVDAEDPECTGPCDNDE
                     KYFSSGLPQQSGGNCRRDCYFDRDAGSGNDRCDWSYTCDKLSIAPAFPPTGESSCAYQ
                     ESASCADLRQTQDAMCLEVCLPITPNGCDCFGCCELPARSNNFVWLGSTRNGSPSCDL
                     AHANDQTACAPCTPVDSCFNHCDDCEVCAGATTVGPGCTGSTPLCSFGTPCGPRAACP
                     GTTYCISGCCVEVPL"
     gene            complement(2346..3023)
                     /locus_tag="VFQ35_19255"
     CDS             complement(2346..3023)
                     /locus_tag="VFQ35_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932854.1"
                     /translation="MSSSDDPTRLRDSALSGEERLAQLFRAAGDDLPSATQLEALAGR
                     LGPVLDAPTPPRVPRTFSKLGALGGTAAVLAIVGGYLVSRSAPHVEPAPLPAHSSSSQ
                     PASPPKQEASAPVAEPVPPPSSSVEVAPVLSAPRGASAKPDEATLLEQARRALASDPR
                     RALALTQRHQALYPRGVLVQEREVIAIEALRRLGREGQASDRANSFEKSYPDSAHRRA
                     VEKGLGK"
     gene            complement(3023..3538)
                     /locus_tag="VFQ35_19260"
     CDS             complement(3023..3538)
                     /locus_tag="VFQ35_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9932855.1"
                     /translation="MSSPGNSPNLRQLFDEHAKYIWRALRHLGVPDADVEDVCQEVWV
                     TAHRKLPEFEGRSSVRTWLYGICMRVASDYRRRAYVRRERPTAEPAPEGEPLATSEFG
                     SRVEARRALKALLELLDEEKRAVLVLYELEGFTMKEVAEILDTPLQTAYSRLYAAREV
                     LSAAVRGEGAE"
     gene            complement(3620..4654)
                     /locus_tag="VFQ35_19265"
     CDS             complement(3620..4654)
                     /locus_tag="VFQ35_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932856.1"
                     /translation="MRGAVARSFALIALLLSSEASASPSTASARLELERSEDARGCID
                     SRRLARAVERRLRRHVFDPNAEATLFVHVVLRRQDAEFVAAIELSDAGGVLGRRELST
                     EARHCSALDDSIALVIALLVESPPERPAPAPEPEAKSPPLEPAASAKGEPPAPSKPVA
                     TIEIPRETLSPREPFGFAIRASALGALGLLPSVSVAPELGFGVRPPRGPWIWATLEAY
                     PPQTERISADAGARLARQRVGLDVCAPEVYFSALSLCLGQRLGTFSASGFGFDHTFER
                     RVLTLAVAAGPDLTFPLGRYFRLFGGARLEVPLSRHRFDAALDDGSVHQLFAEPPAAA
                     NLRVGVDAEL"
     gene            4789..5652
                     /locus_tag="VFQ35_19270"
     CDS             4789..5652
                     /locus_tag="VFQ35_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932857.1"
                     /translation="MADRLFALQVPTASLLLSVAMAAFGCSSATDSDGSDQGGTSPIS
                     LGQGGRNSGSGGSSASSGGGANAAGSGTAAVTACNDGLDNDGDGFVDGFDPECTGAAD
                     NDESSYATGIPGDNRDPKWQDCFFDGNSGAGDDHCRYSTDCLTGKLSPTDPACAITQT
                     CVDFCQRLTPNGCDCFGCCTVELANGSSVNVTLSATCSIDKAGDAKACPRCTKTTQCG
                     NDCGECELCAGKTAADLPASCTPDIPTDGNPPPPTHTCDNGEQVCGPGLPACTGTSSF
                     CSFGCCMPIIR"
     gene            5708..6268
                     /locus_tag="VFQ35_19275"
     CDS             5708..6268
                     /locus_tag="VFQ35_19275"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932858.1"
                     /translation="MANAPSGDHCNPERLIALVRSEDATALDHITRCYGERLLAAGRR
                     HCRTTAEAEDAVQDTLLSFSSDLNQFRGEGSLEGFLVRVVARACRRLSRGHKNDRALH
                     DSEAEPAAVGENPEDGAARTELGAALDTLLLTLEARDRALLLLAELEGYSAAEIGKEL
                     GLSEGAVRTRLSRLRQRLRVDLAAWL"
     gene            6352..6687
                     /locus_tag="VFQ35_19280"
     CDS             6352..6687
                     /locus_tag="VFQ35_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9932859.1"
                     /translation="MTTNDIIPVNALNFESEVLKANIPVLIDVSATWCAPCKAARPVI
                     AELAQRNRGALKVVEIDGDESPDLVAGLGVRGFPTFLGVREGKVVERRAGFGGKRPLE
                     ELAARLITE"
     gene            complement(6716..>8208)
                     /locus_tag="VFQ35_19285"
     CDS             complement(6716..>8208)
                     /locus_tag="VFQ35_19285"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9932860.1"
                     /translation="KNMAYLHAATETAPGHASLFSGRTPREHRIVANEVWRSDLKRRG
                     SLLEDPAARVVSAEGVDPRSTGVSLANVRGQMLADALRERDPKSVVVALSLKDRGAVF
                     AAGRSPTAALWYSEATGKLVTSSAFAKDGKLPAFALPYATVVQPNAPLSWSIFDRAFV
                     EAHQQTPDDLPGESSLFAGSRTFPHEMGGAPAFLGFRFLPEADALLVDLAIASLGLRQ
                     PEHPMLLAVSFSTNDLVGHLFGPTSWEAWSELASLDAQLARLFRGLDAALGPEGYSVV
                     LSGDHGIVPTPEFVKVKQPDYCVKGTDYYERPCVPGMRVVPATLEKLLNTSLQPLLGS
                     NEPSMLPIIDSRIILTDAARALPPIKQKAFDQRLQVLARQDPSVAAVFPIRELPEECP
                     AASDETLRALVCRANTEPAEGFFDVPGDYYVVLKPGRYWGAADGAGHGAPYRYDREVP
                     LLVRYPGGPRERVVEHATFGSYYASVWYALTGEVTGGPYGSVIGAR"
BASE COUNT         1490 a         2709 c         2679 g         1330 t
ORIGIN      
        1 cgtcgtgagc atttgcccat aagggcagat cgagcgatcg ctcgacgtcg acggctgttc
       61 gctgctgcag cgaccttcga gctgaggttt gccgtcggct ccgagctcgg ctctcacgag
      121 cccgggggtt cgctcgtcgg ccgcgaacgc cgactcgaag tcagggtggc gaggtaggtc
      181 cgcgatcggg aagctgacga agtcgcggta ggtcaccgtg agattgagag agttcggaag
      241 actcgccgcg ttctcggcgc aagaaaagcc gttctcgatt cggcaggcac ggtcgcaacc
      301 gtctccgtcg acgttgttgc cgtcgtcgca ttcctccggc gaaatcctga gctggtcgcc
      361 gcagcggggg acacacgccc ctcccgagca ttcgggctcg agcgcacacg cggcggagca
      421 accgtcacca tcgagtgcgt ttccgtcgtc gcaagcctcg gcgccctcgc gaagacgatc
      481 accgcacacc gtcgcgtgac acatcgcggg cccgccttgc ggcggcgcgc acacgaaacc
      541 aggctcgaga cgacacccga cgccgcaccc tttaccgccg ttcggagcat cgcactgttc
      601 gtctcctttc agcgcgccat cgccacaatg cggagtgcac cgtgcccctt ccgaaatgca
      661 gtcgaacccg ggctcgatct cgcagcctgc gctgcatccg tcgccgcccg ttacgttgcc
      721 gtcatcgcac gtttcaccgc cggccaactt gccgtcacca cagcgcacca gattcaagca
      781 aggtcggccg ggctcagggc agacgaagtt cgtttcgacg atccggcagt cagccgagca
      841 cccgtcgccc gcgcggtcgt taccgtcgtc gcacgtctcc cctagcccaa gctccaatcg
      901 gccgttgccg cagccaacgc ggcacgcact gggctcggga cacccgcccg tcgcagacgc
      961 ctcgcctgcc gaattcacgg cgccgccgag cgcgatgccg gtgcttccgc ccgcaccctt
     1021 gccgccgctg ctcgcgccgc tcgttccgcc tcggtcgcgg ccggtattct cgtgcgcaga
     1081 agacggctca gagccggagg gcgaacaacc gagtgaggca gcgcacgcaa aaagggcgat
     1141 gcggtgccat tcgaacattc gggcccgccg ggtgccgtgt aagttcaatc ccttcatcta
     1201 tttctcgctg aaatgccgcg cccggcgatg actcggaggg gtgaccctct gtcgtgtcag
     1261 ggacctccgc tttccgttgc cgggcgacgc gcatttcgtc gcagcgggtg accctccgtc
     1321 gggtcaggcg ccgccggtcc ccgttgccgc gaagcgcgga tttcgaaccc cgccctcatc
     1381 gaggcggcgg gccttctacg gcccaagccc gatcgcgagc actcgctcac ccggatcgac
     1441 tgcgttcaga gcggcacttc gacgcagcaa ccgctgatgc aataggtggt gcccgggcag
     1501 gccgcgcggg ggccacacgg cgtgccgaag ctacagagcg gtgtcgagcc cgtgcagccc
     1561 gggccgacgg tcgtcgcgcc ggcgcacacc tcgcagtcgt cgcagtggtt gaagcacgaa
     1621 tcgaccgggg tacatggggc gcacgccgtt tgatcattcg cgtgcgcgag gtcgcagctc
     1681 ggcgacccat tgcgcgtcga acccaaccag acgaagttgt tgctgcgcgc cggcagctcg
     1741 cagcagccaa agcaatcgca accgttcggc gtgatcggca ggcaaacctc gaggcacatc
     1801 gcgtcttgcg tttggcgaag gtcggcgcac gacgcgcttt cttggtaagc gcaggaggac
     1861 tctccggtgg gagggaacgc cggagcaatc gacagcttgt cgcaagtata tgaccagtcg
     1921 cagcgatcgt tgccgctgcc tgcatcgcga tcgaagtagc aatcccggcg acaatttccg
     1981 ccgctctgct gagggagccc ggaagagaag tacttctcgt cgttgtcgca ggggcccgtg
     2041 cactccggat cttcggcgtc gacgagcccg tcgccgtcgt tgtccatgca atcgccgcac
     2101 tggtacggcg ccttgttgct ctcgcaccga gtgaccttgc acgccggcgc gcctgcgacg
     2161 ccgccgctgc cggagttcgc actgcccgcg ctccccccga ccgtcgagat gccacccgcg
     2221 gaaaggtcat cgtagccgac gcgcaagctc ggctcgctac aggctccgaa caacagggcg
     2281 agcgagacgc tcgcaccgaa aaggcatcga gaaagcccca tggcgccttc agctcatccc
     2341 atggctcact tgccgagtcc cttttctacc gcgcgacggt gcgccgagtc gggatagctc
     2401 ttctcgaaac tgttggcgcg atccgacgct tgtccttcgc ggcccagccg gcgcaaggct
     2461 tcgatcgcga tgacctcgcg ctcctgcacc agcactccac gcgggtaaag ggcttgatgg
     2521 cgctgagtga gcgcaagagc acggcgggga tcgctagcga gcgctcgacg ggcctgttcg
     2581 agcagcgtgg cctcgtcggg ttttgcagac gcaccccttg gtgcggagag caccggcgcc
     2641 acctcgacgc tcgaggatgg cggcggcacg ggttcggcga ccggggccga agcctcctgc
     2701 tttggcggag atgctggctg agacgaactc gaatgtgccg gtaaaggtgc cggctcgacg
     2761 tggggggctg agcgcgagac gaggtagccg ccgacgatcg cgagaacggc agcggtgccg
     2821 ccgagcgcgc cgagcttgga aaacgtgcga ggcactcgcg gcggcgtcgg tgcatcgagt
     2881 acaggcccga gccgcccggc tagcgcctcg agctgcgtcg cggatgggag atcgtctccg
     2941 gcagcgcgaa acagctgggc gagccgctct tcacccgaga gcgccgagtc gcgcaaccga
     3001 gtggggtcgt ccgagctcga catcactcgg ctccttcgcc gcgaacggcc gcggacaaga
     3061 cctcgcgcgc ggcatacaag cgcgagtacg ccgtttgcaa cggcgtatcc agaatctcag
     3121 ccacctcttt catcgtgaac ccttcgagct cgtagaggac cagcaccgcg cgcttctcct
     3181 catcgagcaa ctcgagcagc gccttgagtg ctcgacgcgc ttcgacgcgg ctaccgaact
     3241 ccgaagttgc gaggggctca ccctccggcg cgggctcggc tgtggggcgt tcacgtcgaa
     3301 cgtaggctct cctgcgatag tccgaagcga cccgcatgca gatgccgtag agccacgtgc
     3361 ggacgctcga tcgcccctcg aactcgggaa gcttgcggtg cgccgtgacc caaacctctt
     3421 ggcagacgtc ttcgacgtcc gcatcgggaa cgccgaggtg gcgcagcgca cgccagatgt
     3481 acttcgcgtg ttcatcaaag agctgccgca ggttggggct gtttccaggc gagctcattc
     3541 tggaccttcg cgctgctcgc gtccgagagg attcactgag ctcgtaaggg tgagtggggg
     3601 catcgtccga aaattcggat cagagctccg cgtcgacccc gacccggagg tttgccgcag
     3661 caggcggttc cgcgaacaac tggtggaccg agccgtcgtc gagcgcggca tcgaaccgat
     3721 ggcgagacaa gggaacctcc aagcgagctc caccaaatag ccgaaagtac cgtccaagcg
     3781 gaaatgttag atcaggcccc gccgccaccg ccaacgttag gacacggcgc tcgaaggtgt
     3841 gatcgaaccc gaatcccgac gctgaaaaag tcccgaggcg ctgcccgagg cacaacgaga
     3901 gcgcggaaaa gtaaacttcc ggagcgcaca catcgaggcc cacccgctgg cgagcaagcc
     3961 gagcgcctgc atccgctgaa atcctttcgg tttgaggagg gtacgcttcg agcgtggccc
     4021 atatccacgg gccccgtggc gggcgaaccc caaaacccag ctcgggtgcg acgctgacgc
     4081 tcggaaggag cccgagcgcg ccgagcgcgg aagcgcgaat tgcgaagccg aacggctcac
     4141 gtggcgacag cgtctcgcgc gggatttcga tcgtggccac cggtttagac ggcgcaggag
     4201 gctcgccctt cgcggaagcc gcaggctcca gcggcgggct cttcgcctcg ggctctggcg
     4261 cgggcgcagg gcgctcggga ggcgattcga cgagcagcgc gatgacgagc gcgatcgaat
     4321 cgtccagcgc cgaacagtgg cgagcttcgg tcgaaagttc tcgacgaccg agcacgccac
     4381 ccgcgtctga aagctcgatc gccgccacga actccgcatc ctgtcggcga agcacgacat
     4441 gaacgaacag cgtggcttcg gcgttcggat cgaagacgtg tcgacgtaaa cggcgctcga
     4501 ccgcgcgagc aaggcgcctc gaatcgatac aaccgcgagc gtcttcactg cgttcgagct
     4561 cgagacgcgc ggacgccgtg gacggagaag cagaagcctc gctcgagagc agaagcgcga
     4621 tgagagcgaa gctccgagcg accgcgccac gcatggcgcc tcactctagc gtcccatctg
     4681 ccgaaaatgg ctgatttttt tcgagagaag cagcgagaga acctcaatgg ccgggtcggt
     4741 tcagaaaatc tgcaaccccc tccccactca cttcacatga caagaaggat ggccgaccga
     4801 ctcttcgcac tccaagtccc cacggcgtct ttgctgttga gcgttgcgat ggccgccttc
     4861 ggctgttctt ccgccacgga ctcggatggc tcggaccaag gaggaaccag ccccatcagc
     4921 ttggggcaag gcggtcggaa ttcggggtcc gggggaagct ctgcgagctc gggcggaggg
     4981 gcgaacgcgg ccgggagcgg gactgcggcc gttaccgcct gcaacgacgg cttggacaac
     5041 gatggcgacg gcttcgtcga cggcttcgat ccggagtgta ctggcgcagc agacaacgac
     5101 gagagctcgt atgccacggg gattccagga gataaccgcg acccgaagtg gcaagactgc
     5161 tttttcgacg gcaactcggg cgcgggagat gaccactgtc gatacagcac cgattgcctg
     5221 acgggcaagc tgtccccgac cgaccccgcg tgcgcgatca ctcagacgtg cgtcgacttc
     5281 tgtcagcgac tcacgcccaa cggttgcgat tgcttcggtt gttgcacggt ggagctagcc
     5341 aatggctcgt ccgtgaatgt gacgctcagc gcaacgtgct ccatcgataa agcgggagac
     5401 gcgaaggcgt gcccacgctg caccaagacc acccaatgcg gaaacgactg cggcgaatgc
     5461 gagctctgcg caggcaagac ggcagccgat ctgcccgcga gctgcacgcc cgacatcccg
     5521 accgacggaa acccgccgcc cccgacccac acctgcgaca acggtgagca ggtttgcggg
     5581 cctggcttgc cagcttgtac cggtacgtcg agcttctgct ctttcggctg ctgcatgccg
     5641 atcatccgat gaatggagcg aagcgacatc acctggtgag cggtgcatcc ggtgataagg
     5701 ttcgcgagtg gcgaacgctc cgagcggtga ccattgcaat cccgagcgcc tcattgcact
     5761 cgttcggagc gaagacgcta cggcgctaga tcacatcaca cgctgttacg gagagcgctt
     5821 gctcgcggcc gggcggcgcc attgtcgcac caccgccgag gcagaagatg ccgtgcaaga
     5881 cacgctgctc tcgttctcga gcgacctgaa tcagtttcgc ggcgagggca gcctcgaagg
     5941 ctttttggtc cgcgtcgtcg cgcgtgcgtg tcgacgactc tctcgaggtc acaagaacga
     6001 ccgcgctctc cacgatagcg aagcagaacc cgcggcagtg ggcgagaacc ccgaggacgg
     6061 cgccgcgcgc accgagctcg gggctgcact cgacacgctc ttgttgacgc tcgaagcgcg
     6121 cgaccgtgcc cttctcttgc tggccgagct cgaaggctac tcggcggcgg aaatcggcaa
     6181 ggagctcggt ctgagcgaag gcgcagtccg cacgcggttg agccgccttc gtcagcggct
     6241 gcgggtggac ttggccgcgt ggctctgagc accgaagtga acgccgaccc cctggtcacc
     6301 gaaaagatct cgaggctgta acgaatcggc gagtcgttgg cccttgcggg catgaccacg
     6361 aacgacatca tccccgtgaa cgcattgaac ttcgagagcg aggtcctgaa agcgaatatc
     6421 cccgttttga ttgatgtttc ggcaacttgg tgcgcgccgt gcaaggccgc ccggcccgtc
     6481 atcgccgagc tcgctcagcg aaatcggggc gcgctgaagg tcgtcgagat cgacggagac
     6541 gagtccccgg atctcgttgc tgggctcgga gtacgcggct ttccgacctt cttgggcgtg
     6601 cgggagggga aagtcgtcga acggcgcgcc gggttcggcg gcaagcgtcc gctcgaggag
     6661 ctcgcagcgc ggctcatcac cgaatagctt gcgagggcac cgcacacgcg ctgcgctagc
     6721 gagcgccgat caccgaaccg taggggccac cggtgacctc accggtcagc gcataccaga
     6781 ccgatgcgta atagctaccg aaagtggcgt gctctacgac gcgctcgcgc gggcccccag
     6841 ggtacctgac cagcaacggg acttcgcgat cgtagcgata cggggcaccg tgaccggcac
     6901 cgtctgccgc cccccagtac cgccctggct tgagaacgac gtagtagtcc ccgggtacgt
     6961 cgaagaagcc ctctgccggt tcggtgttgg cgcgacaaac gagcgcgcgc agcgtctcgt
     7021 cactggctgc gggacactcc tcggggagtt cgcggatcgg gaacacggcc gcgacgctcg
     7081 ggtcttggcg cgccagcact tgcagtcgct ggtcgaaggc cttttgcttg attggcggca
     7141 gcgcgcgcgc agcgtcggtc aggatgatgc gcgagtcgat gatcggtaac atcgaaggct
     7201 cgttcgagcc gagcagcggt tggaggctcg tgttcaggag cttctccagc gtcgccggga
     7261 cgacgcgcat gcccgggacg caggggcgct catagtagtc ggtccccttc acgcagtaat
     7321 ccggctgctt cactttgacg aactccggcg tcgggacgat gccgtggtct ccgctcagca
     7381 ccaccgagta cccttccggc ccgagcgcgg cgtcgagccc tcgaaacaga cgcgcgagct
     7441 gcgcatcgag agaagcaagc tcactccacg cttcccacga cgtgggcccg aacaagtgtc
     7501 caacgagatc gttcgtcgaa aacgaaacgg cgagcagcat ggggtgctcc ggctggcgca
     7561 agccgagcga agcgatggcc aaatcgacga gcagcgcgtc tgcttcgggg aggaaacgga
     7621 atcccaaaaa cgccggcgct ccgcccatct cgtggggaaa cgttcggctc ccggcgaaga
     7681 gcgatgattc acccggcaga tcatccggcg tctgctgatg tgcctcgacg aacgcgcgat
     7741 cgaagattga ccagctgagc ggcgcgttgg gttgaacgac agtggcgtac ggcaaggcga
     7801 acgccgggag cttgccgtct ttggcgaacg cgctcgaggt gacgagcttt ccggtcgctt
     7861 cggagtacca gagcgcggcg gtcggggagc ggcccgccgc gaacacggcg cctcgatctt
     7921 tcaacgagag cgcgaccacg accgacttcg ggtctcgctc gcgcaaagcg tcggcgagca
     7981 tttggccccg cacgttggca agtgaaaccc cggtgctcct cgggtcgacg ccttcggcgc
     8041 tcacgacgcg tgcggcggga tcttcgagca atgagccgcg ccgcttcaag tcgctgcgcc
     8101 agacctcgtt cgcaacgatg cgatgttccc gcggggtccg accgctgaac agcgacgcat
     8161 ggccgggcgc cgtttcggtc gccgcgtgca ggtacgccat gtttttca
//
LOCUS       DASTKS010000920         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_147740, whole genome shotgun sequence.
ACCESSION   DASTKS010000920 DASTKS010000000
VERSION     DASTKS010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147740"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFQ35_19290"
     CDS             complement(<1..956)
                     /locus_tag="VFQ35_19290"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9932861.1"
                     /translation="MAHANRLATETSPYLLQHAQNPVDWYPWGEEALERARRENKPIF
                     LSIGYAACHWCHVMERESFENQQVADYLNAHFVSIKVDREERPDIDDIYMAATLALSG
                     SGGWPMSVFLTPEREPFFAGTYFPPDGRYGRPGFLSLLSRIVEAWEKEPKALFEQAKR
                     LTHHIRERSRPVPARGLSAANIERAAAEYARSFDEKWGGFSPAPKFPSHQGLRLLMRH
                     HAKSGDAHTLHMVRRTLDAMKNGGIYDQVGGGFARYSTDERWLVPHFEKMLYDNAQLA
                     IVYLEAFQISAEPEYQRIARETLDYVVREMQSPAGGYYSATDA"
     gene            complement(981..2234)
                     /locus_tag="VFQ35_19295"
     CDS             complement(981..2234)
                     /locus_tag="VFQ35_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9932862.1"
                     /translation="MRPPRDQPSKTLPEVVKRRRALTHVGGEADIVAESDELRNVVAR
                     ALDVSDEDERVLAHVHGFHSYPARLHAETAKTLIEALSSRGDSVLDPFCGSGTVPVVA
                     RELGRRAFGSDLNPLAVELARLKAAGMSESFARALVAASARVVEHARARQEAELGPTH
                     PYGAEDRKEFATHVLLALDGLRDGIDEIEERGVRHALLLVLSACLTKLSEKKADSSSV
                     HQPKRIARSFPFRFFALKTEDLARRALEFTARVPARTPAAELEICDARRLAYLPSKRV
                     KLVVSSPPYPGVYDYFEHHATRLRWLRLDGTRLEQGEIGTRRAARRDAARAAGEWEAD
                     FGRCLVELRRVLTQDGLAALMLADSVIGARPYYADEWLPRVAKHERVAIVGRGSQARP
                     HFHAPTARAFQRRPRREHLFILAPR"
     gene            complement(2316..3317)
                     /locus_tag="VFQ35_19300"
     CDS             complement(2316..3317)
                     /locus_tag="VFQ35_19300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9932863.1"
                     /translation="MEPTRAPSAAVRPRVEARTPPRPSSRRFGGYVALGAALAALAVA
                     AVSTTREEAARGPHQGAAGLKHTTKGAEVRWRLQSTTVRIDSSLSHLGSGAENAVKQA
                     FTTWTQSEAALPRVKFETTSKAEAQAAPDGKNTVVLAPITVKGHEHDLAITLTYSDEQ
                     TGDIVEADIVVNSEYPFKLLAMSAENDLEDDDSSNGNGSPTTTTGTQPSVNSARASCQ
                     ATAKAPSCDGGAYDLQNVVTHEVGHFFGLGEDMDDATSTMYYCTSRCETHKRVLTNDD
                     TTVMNVLYAPSTATDGSPASAGGCGGARIAPRGGLAASAWMVATALLGLTLRRRRAR"
     gene            3542..>4522
                     /locus_tag="VFQ35_19305"
     CDS             3542..>4522
                     /locus_tag="VFQ35_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9932864.1"
                     /translation="MPSVATRRLIQERLDAETGRIDKEAPTRVALTYPSPYHVGMSSL
                     GYQQVYKGLQAMPGVACERVFLPDDAADPSGRVAERPVSYEHRRELSEFPLILVSVAY
                     ELELAGLIKLLDSSQIPALARDRRERDPLVIAGGPLTFSNPLPLGPFVDAIVIGEAEE
                     LLEPVVRAASEAPTRAEALEALAKLPSVFVPALHGEALPTPAACDDQLLPAFSTIRTP
                     HTELSNMFLIEAERGCSRGCTYCVMRRSTNGGMRIVPKDVVLERIPSDVRRVGLVGAA
                     VSDHPKIVEILHALADRECEVGLSSLRPDRLKDEFVSALRRVGYRTLTTAL"
BASE COUNT          692 a         1479 c         1540 g          811 t
ORIGIN      
        1 gcgtctgtcg cggagtaata gccgcccgcg gggctttgca tctcgcggac gacgtagtcg
       61 agggtctcgc gagcgatgcg ctggtactcg ggttcggcgc tgatctgaaa cgcctcgagg
      121 tagacgatcg cgagttgggc gttgtcgtac agcatcttct cgaaatgcgg cacgagccat
      181 ctttcgtcgg tggagtagcg agcgaagcct ccgcccacct gatcgtagat accgccgttc
      241 ttcatcgcgt cgagcgtgcg acgcaccatg tgtagcgtgt gcgcgtcgcc gctcttcgcg
      301 tggtgtcgca tcaagaggcg cagcccctgg tgcgagggga acttcggcgc ggggctgaag
      361 ccgccccact tctcgtcgaa ggagcgggcg tactcggcgg cggcccgctc gatgttcgcc
      421 gcgctgaggc cgcgagctgg aactggacgc gaacgctcgc ggatgtgatg ggtgaggcgc
      481 ttcgcttgct cgaagagcgc tttgggctcc ttttcccaag cctccacgat gcgcgaaagc
      541 agcgacaaga agccgggtcg cccgtagcgc ccatcgggtg ggaagtacgt gcccgcgaag
      601 aaaggctcgc gctccggcgt caggaaaacg ctcatgggcc agccgccgct ccccgagagt
      661 gccaatgtcg cggccatgta gatatcgtcg atgtcgggcc gctcttcgcg atccaccttg
      721 atgctcacga agtgagcgtt caagtagtcg gcgacctgtt ggttctcgaa cgactcgcgc
      781 tccatcacgt ggcaccagtg gcacgccgcg taaccgatgc tcagaaagat tggcttgttc
      841 tcacgccgcg ctcgctcgag cgcttcttcc ccccaggggt accagtcgac cgggttctga
      901 gcgtgctgca agagataggg tgaggtttcc gttgcgagtc ggttcgcgtg agccataagt
      961 cgagtctttt tggcgcgctc tcagcgcggg gccaagatga acaaatgctc tcggcgcggc
     1021 cggcgttgga acgcgcgggc cgtaggagcg tgaaaatgcg gacgcgcttg gctgcctcta
     1081 ccgacaatcg cgactcgctc gtgcttcgcg acgcgcggca gccactcgtc ggcgtagtag
     1141 gggcgcgctc cgatgaccga atcggcgagc atcagcgccg ccaagccgtc ttgcgtgagc
     1201 actcgccgta gctcgacgag gcaacggccg aaatcggctt cccactcacc agcggctcgc
     1261 gccgcgtcac gccgggccgc gcgccgagtg ccgatctcgc cctgttcgag gcgcgttcca
     1321 tccaggcgta accagcgcaa gcgcgtcgcg tggtgctcga agtagtcgta aacgcccgga
     1381 tacggtgggg agctcacgac gagcttcact cgtttgctcg gaaggtacgc gagccggcgc
     1441 gcgtcgcaga tctcgagctc cgcggctggc gtgcgggccg ggacgcgcgc cgtgaattcg
     1501 agcgcgcgcc gggcgaggtc ctccgtcttc agggcgaaga agcgaaacgg aaagctgcga
     1561 gcgatgcgct tcggttgatg cacgctgctc gagtcggcct tcttctcgct cagcttggtt
     1621 aggcacgccg agagcaccag caggagcgcg tgccgcacgc cacgctcctc gatctcgtcg
     1681 atgccgtcgc gcaggccgtc gagcgcgagc agcacgtggg tcgcgaactc tttgcgatct
     1741 tccgcgccgt aggggtgagt cggcccaagc tccgcttctt gtcgagcgcg cgcgtgctcc
     1801 acgacgcgcg ccgatgccgc gaccagcgcg cgcgcgaacg actccgacat cccggccgct
     1861 ttcaggcgag cgagctcgac tgcgagcgga ttcaagtcgc tcccgaacgc gcggcgcccg
     1921 agctcgcggg ccacgaccgg caccgtgccg ctgccgcaga acggatcgag cacggagtcg
     1981 ccccgcgacg agagcgcttc gatcagcgtc ttcgcggttt cggcgtgcaa gcgcgcgggg
     2041 tagctgtgaa agccgtggac gtgggcgagc acgcgttcgt cctcgtcgct cacgtcgagg
     2101 gcgcgcgcga cgacgttccg gagctcgtcg ctttcggcga cgatatcggc ctctcccccc
     2161 acgtgcgtca gcgcgcgacg gcgcttgacg acttcgggca aggtcttcga cggctggtct
     2221 ctcggggggc gcaccgggcg agcttgtcgc ggccgaggcg attcgtaaag gcagcgagcc
     2281 tccttcccga gttcgctcgg gacggatggc gtcgttcagc gcgcccggcg gcggcggagc
     2341 gtcagaccga gcagcgcggt cgcgaccatc caggccgaag cggcgagccc accgcgcggc
     2401 gcgatgcgcg cgcctccgca gccgcctgcg ctcgcggggg aaccgtcggt cgcggtagac
     2461 ggcgcgtaaa gcacgttcat caccgtcgtg tcgtcgttcg tcagcacgcg cttgtgcgtc
     2521 tcgcaacggc tcgtgcagta gtacatggtg ctggtagcgt cgtccatgtc ctcgccgagg
     2581 ccgaagaagt ggccgacctc gtgggtcacc acgttctgca agtcgtacgc gccgccgtcg
     2641 cagctcgggg ctttggccgt cgcctgacac gacgcccgcg ccgagttgac gctcggctga
     2701 gtgccggtgg tcgtggtcgg gctaccgttt ccgttgctcg agtcgtcgtc ctccaggtcg
     2761 ttctcggcgg acatcgcgag aagcttgaag gggtactccg agttcaccac gatgtcggct
     2821 tcgacgatgt cgcccgtttg ctcgtcggag tacgtgagcg ttatcgcgag gtcgtgctcg
     2881 tggcccttca cggtgattgg cgcgagcacg accgtgttct tgccgtcggg cgcagcctgc
     2941 gcttcggcct tgctcgtggt ttcgaacttc acgcgcggta gcgcggcctc cgattgagtc
     3001 caagtcgtga aggcttgctt caccgcgttc tccgcgcccg aaccgaggtg cgagagcgac
     3061 gagtcgatgc gcacggtcgt gctctgcaag cgccaacgga cctctgcgcc cttcgtcgtg
     3121 tgcttgagtc cggccgcgcc ctggtgcggg ccgcgcgctg cttcctcgcg cgtcgtcgag
     3181 accgcggcga cggccagcgc tgcgagcgcg gcgccgaggg cgacgtagcc accaaaacgg
     3241 cgcgaactcg gacgaggagg cgttcgcgct tcgactcgag gtcggacggc tgcgctcggc
     3301 gcccgagtcg gctccaccaa ggcggataag ctggttattg gtggggggcg gaagctcggc
     3361 gctgcggagg ttggatgaac ggaagccgtc acgcattcat gaacggcaat caacgctcgc
     3421 gtcttgagcg caaagcagca ggaaaagtgg cacgattcgc ataatttgga ggggttattg
     3481 tcgtttctca gcacctgatt cccgacgccc gtcgcgtgag gcgctataac gtgcggctcc
     3541 aatgcccagc gttgccaccc gccgtctcat tcaggagcgt ctcgacgcag agaccgggcg
     3601 catcgacaag gaggcaccga ctcgcgtcgc gctcacctac ccttcaccct atcacgtggg
     3661 gatgagctcg ctcggttacc agcaggtgta caagggccta caggcgatgc ccggtgtcgc
     3721 ctgcgagcga gtgtttcttc ccgatgacgc tgctgaccct tcgggtcggg tcgcggaacg
     3781 tccggtgagt tacgagcacc ggcgagagct ctccgagttc cccctgattt tggtgagcgt
     3841 cgcgtacgag ctcgagctcg cgggcctcat caagctgctg gactcctcgc aaattccagc
     3901 gttggcgcgc gatcgccgcg aacgggaccc gctcgtcatc gccgggggac cgctaacctt
     3961 ctcgaacccg ctgccgctcg gacccttcgt cgacgcgatc gtgatcggcg aggccgagga
     4021 gctactcgag cccgtcgtgc gcgctgcctc cgaagcgccg acgcgcgccg aagcactcga
     4081 ggcgctcgcg aagctcccga gtgtgttcgt gccagcgctc cacggcgagg cgcttccgac
     4141 gccggcggct tgtgacgacc aactgctccc agcgttcagt acgatccgca cgccgcacac
     4201 cgagctctcg aacatgttcc tcatcgaggc ggagcgcggc tgctcgcgcg gctgcacgta
     4261 ctgcgtcatg cggcgcagca cgaacggggg catgcgcatc gttcccaagg acgtcgtctt
     4321 ggagcgtatt ccgagcgacg tgcgccgcgt cgggctggtc ggcgcggccg tcagtgatca
     4381 cccaaaaatc gtggagattt tgcatgccct ggccgatcgc gagtgcgagg tggggctctc
     4441 cagcttgcgg cccgatcgtc tcaaggacga gttcgtcagc gcgttgcggc gtgtcggcta
     4501 ccgcacgttg acgaccgctc tc
//
LOCUS       DASTKS010000921         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_816548, whole genome shotgun sequence.
ACCESSION   DASTKS010000921 DASTKS010000000
VERSION     DASTKS010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816548"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..2038)
                     /gene="tssF"
                     /locus_tag="VFQ35_19310"
     CDS             complement(164..2038)
                     /gene="tssF"
                     /locus_tag="VFQ35_19310"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HET9932865.1"
                     /translation="MDPRLLAYYNRELHYLRELGGEFAKQFPKIAGRLGLETFECADP
                     YVERLLEGFAFLAARVQLKIDAEFPRFTEHLLTMVYPHYLAPTPSMAVVRLLPNHRQG
                     VLNEGFRVPRDTALRSTVGRGEQTACEYRIAHDVTLFPIELASVTHTSYVGDLGDLKI
                     PGSKPVRGALRFRLRTLNGARINQLRLDTLPLFLSGTEQISTRLYELLFAGTQGIIVR
                     SVNGTSRTLISKQPLRPLGFEDDESLLPYTAKSFQGYRLLQEYFALPQRFLFAELCGL
                     LEGIQKVGETEVEIVVLLDRHDPVVEAAVAPTHFELFCTPCVNLFPKRVDRIHLSDRV
                     SQYHVVTDRTRPLDFEVHSVTEVLGYGGNTEVRREFQPFYACKDRTVVAEDPGAYYTL
                     HRETRAASARQRRAGPRSTYPGTEVFISLVDGNQGPHSPDLKQLALNTLCTNRDLPLQ
                     MNLGLGSTDFTLESGAPVDTVRVVAGPSEPRPSSAWGANSWRFVSHLSLNYLSITERS
                     EGDGGAGLRELLHLYGDFANTATRRQIDGVRGVSSSHVVRRLPVEGPVNFGRGLEIRI
                     DCDESAFEGASVFLFGAVLERFFAKYASINSFTETVLRTLQRGEIMRYTTRVGRRAIA
                     "
     gene            complement(2081..>2418)
                     /gene="tssE"
                     /locus_tag="VFQ35_19315"
     CDS             complement(2081..>2418)
                     /gene="tssE"
                     /locus_tag="VFQ35_19315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HET9932866.1"
                     /translation="TVQDLDAYPYVEQSTLNYGMPDLAGKTASTVDSSALEKSIRRAI
                     WEFEPRLLKESVRVKAVVNDKQMNHNALCFTIEAELWAQPLPLRLFLRTDVDLETGSV
                     VVTEAARTD"
BASE COUNT          438 a          767 c          799 g          414 t
ORIGIN      
        1 ggggcgaacg cgagcgtcgg ctcttgcccg acccgcagca cctcgtcgga gggacgcacc
       61 gcttctccaa agcgcggcag gtttcggtaa aggtgctcga gccggcgcaa caccaagtga
      121 aagtcgaact cgaagggggc ggccgcgagc tcgctgagaa agctcatgcg atcgcccgcc
      181 ggcccacgcg cgtcgtgtag cgcatgattt caccgcgctg cagggtgcgc agcaccgtct
      241 ccgtgaagga gtttatcgat gcgtacttcg cgaaaaaacg ctcgagcacg gcgccgaaga
      301 ggaagacgct cgccccttcg aaggcgctct cgtcgcagtc gatgcggatc tcgagcccgc
      361 gcccgaagtt caccggaccc tcgacgggca gccgccgcac gacgtggcta ctcgagacac
      421 ctcgtacccc gtcgatttgt cggcgcgtgg cggtgttcgc gaaatcgccg tagaggtgca
      481 agagctcgcg cagccccgcg ccaccatcgc cctcgctgcg ttcggtgatc gagaggtagt
      541 tgagcgagag gtggctcacg aagcgccagc tgtttgcgcc ccaggcgctc gacggtcgcg
      601 gctccgaagg cccggcgacg actcgcaccg tgtcgacggg ggcgcccgac tcgagcgtga
      661 aatcggtcga gccgaggccg aggttcattt gcagcggcag atcacggttc gtgcagaggg
      721 tgttcagcgc tagctgcttg aggtcggggc tatgcgggcc ctggttgccg tccacgagcg
      781 agatgaacac ctccgtgccc gggtacgtgg agcgcgggcc ggcgcggcgc tgacgagcgg
      841 aagcggctcg cgtctcgcgg tgcagcgtgt agtaggcgcc cggatcttcc gctacgaccg
      901 tgcgatcttt gcaggcgtag aagggttgga actcgcgccg gacttcggtg ttgccgccgt
      961 agcccaggac ctcggtcacg ctgtgcacct cgaagtcgag cgggcgcgtg cgatccgtga
     1021 cgacgtgata ttgactcacg cgatccgaca ggtgaatgcg atcgactcgc ttcgggaaga
     1081 ggttcacgca cggggtgcag aagagctcga aatgcgtggg cgcgacggcc gcctcgacga
     1141 ccggatcgtg ccgatcgagc agcacgacga tctccacctc ggtttcgccg accttctgga
     1201 tgccctcgag caacccacag agctcggcaa acaagaagcg ctgcggcagc gcaaagtact
     1261 cttgcaggag ccgatacccc tgaaacgact tggccgtata ggggagcagg ctctcgtcgt
     1321 cctcgaaacc gaggggacgg agcggctgct tactgatgag cgtgcgagac gtcccgttta
     1381 ccgaccgcac gatgatcccc tgtgttcccg cgaacaggag ctcgtagagg cgcgtcgaga
     1441 tctgctccgt gccgctcagg aatagcggca acgtgtcgag cctgagttga ttgatgcgcg
     1501 cgccgttcag cgtgcggagt cggaaccgca acgcgccgcg gaccggtttc gagccaggga
     1561 tcttcagatc gccgaggtca ccgacgtacg aagtgtgcgt gacgctcgcg agctcgatgg
     1621 ggaacagggt cacgtcgtgc gcgatgcggt actcacaagc ggtctgctcg ccccggccga
     1681 cggtgctgcg cagcgccgtg tcacgcggca cccgaaatcc ctcgttcaag acgccttgcc
     1741 gatgattcgg caagagccgc accaccgcca tcgagggagt cggcgcgaga tagtgtgggt
     1801 acaccatcgt cagcaggtgc tcggtgaacc gcggaaactc cgcgtcgatt ttcagctgga
     1861 cgcgcgcagc gaggaacgcg aacccctcga gcaagcgctc cacgtagggg tcggcgcact
     1921 cgaaagtctc gagcccgagc cgcccggcga tcttggggaa ctgcttcgcg aactctccac
     1981 ccagctcgcg caggtagtgg agctctcggt tgtaataggc gagcagccga ggatccattt
     2041 cccgttatcc tatgcgcctt ggcatactgc gcccgcgcgc tcagtccgtt cgcgccgctt
     2101 ccgtgacgac cacggacccg gtctcaagat cgacgtcggt gcgcaagaac aatcgaagcg
     2161 gcagcggttg cgcccacagc tcggcttcga tcgtgaagca gagagcgttg tggttcatct
     2221 gcttgtcgtt gaccaccgcc ttgaccctga ccgactcttt caaaagccgc ggctcgaact
     2281 cccaaatcgc gcggcgaatg gatttctcga gcgcgctgct atcgacggtc gacgccgttt
     2341 tgccggccag atcgggcatg ccgtagttga gcgtcgactg ctcgacgtac ggatacgcat
     2401 ccagatcttg caccgtcg
//
LOCUS       DASTKS010000922         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_593853, whole genome shotgun sequence.
ACCESSION   DASTKS010000922 DASTKS010000000
VERSION     DASTKS010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593853"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFQ35_19320"
     CDS             <1..330
                     /locus_tag="VFQ35_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932867.1"
                     /translation="EQLSWTNEGALATSSASTYKLPTLGECPPIINVALLERAAEPGV
                     VYGSRAVGEPPFMLAISVREALRAAVAAFGAQGVVELASPATPEAVFWAIEKGRSRRQ
                     PRARAAE"
     gene            complement(354..1571)
                     /locus_tag="VFQ35_19325"
     CDS             complement(354..1571)
                     /locus_tag="VFQ35_19325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9932868.1"
                     /translation="MKLSSRKICKWLGITLAAVTCIVGALAGRARWVVSRSYGFVAKP
                     PITADRSAVGVARGELLFQSLCIECHRGVDGRATGKHLAEVPEFLGKFYSANLAHPEH
                     GVRRRSDAELARVLRFGVLPDGRLSAAMNGFGKLGDADVAALLGYMRSDADVFAPAGG
                     ETPRTEISLLGSMILTYVAQINVDARASGVPVPPKSVSVEYGRYMVEALDCVGCHTEG
                     FSSAKMQHEKAFAGGFEFVDPTGAKIWSKNITFDESTGIGSWSIDDFERAVTRGITPA
                     GYMVRKPMPLFARLDRTDVAAIYAFLRSMPRVERRNTPGGHPLKKAAPGDSPEMLFVN
                     VGCAACHGKAGPYRSKLTGALAKSDDEVASWILDPQSRKPGSAMPSFQHALNRTQAEA
                     LARYVKTIAQEGG"
     gene            complement(1616..>2048)
                     /locus_tag="VFQ35_19330"
     CDS             complement(1616..>2048)
                     /locus_tag="VFQ35_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932869.1"
                     /translation="APELQFRSNLAAGFREMSARGTISLWMTAAEVAAAQSSGLRVVV
                     HDLDPTDPRPPEIPADADLFITRVRLDLAGVKALDVEVLTPDGKLVKCMPDIAFDPAE
                     GLIYACCEAELARVAASKRTVTRLWATYENGRRLLGELDLA"
BASE COUNT          351 a          662 c          696 g          339 t
ORIGIN      
        1 gagcagcttt cgtggacgaa cgagggagcg ctcgcgacgt cgtccgcgtc gacctacaag
       61 cttcctacgc tcggggagtg tccgccgatt atcaacgtcg ccctgctcga gcgagcggcg
      121 gagccgggag tcgtgtacgg gagcagagcg gtgggagaac cgcccttcat gctggcgatc
      181 agcgtgcgcg aggctttgcg cgcggcggtg gctgcgtttg gggcgcaagg agtcgtggag
      241 ctcgcgtcgc ccgcgactcc agaagcggtg ttttgggcca tcgagaaggg ccgtagtcgg
      301 cgccaaccgc gtgcacgcgc ggcggagtga cgcggcacct cccgtcatcc ccgtcagccc
      361 ccttcctggg cgatggtctt cacgtaacga gcgagcgctt cggcttgggt ccggttcagg
      421 gcgtgctgaa atgaaggcat cgcagagccg ggcttgcgcg attggggatc gaggatccac
      481 gaggcgactt cgtcgtcgct cttcgcgagc gcgccggtga gcttgctgcg atacgggccc
      541 gctttcccgt ggcacgccgc gcagccgacg ttcacgaaga gcatttcggg cgagtcaccg
      601 ggcgcagctt tcttcagcgg atgaccgccg ggagtgttcc gtcgctcgac cctcggcatg
      661 ctgcgcaaaa aggcgtaaat ggcggcgacg tcggtgcggt cgagccgcgc aaagagcggc
      721 atgggttttc gcaccatgta gccggctggc gtaatccctc gggtgacggc gcgctcgaag
      781 tcgtcgatcg accagctacc gatccccgtc gactcgtcga acgtgatgtt cttgctccag
      841 atcttggcgc cggtcggatc gacgaactcg aaaccacccg cgaacgcctt ttcgtgctgc
      901 atcttggccg agctgaagcc ttcggtgtgg caaccgacgc agtcgagcgc ctctaccatg
      961 tagcgtccgt actccacgct caccgacttc ggcggcaccg gcacgccgct cgctcgcgca
     1021 tcgacgttga tctgcgcgac gtaggtcaaa atcatcgatc cgagcagcga aatctcggtg
     1081 cgcggtgtct ctccgccggc gggtgcgaac acgtcggcgt cggagcgcat gtaaccgagc
     1141 agggccgcga cgtcggcgtc cccgagcttg ccgaagccgt tcatcgccgc cgagaggcga
     1201 ccgtcgggca gtactccgaa gcgtagaacg cgcgcgagct ccgcatcaga gcgccgccga
     1261 accccgtgct ccgggtgcgc caaattcgcc gagtagaact tgccaaggaa ctccggaact
     1321 tcggccaggt gcttgcccgt ggcgcgcccg tccacgccgc gatggcactc gatgcagagg
     1381 gactgaaaca agagctcgcc acgggcgacg ccgacggccg agcgatcagc ggtgatcggt
     1441 ggtttcgcga cgaaaccgta gctacgcgac acgacccagc gggctcgccc cgcgagagcg
     1501 ccgacgatac atgtcaccgc ggcgagcgtg atcccgagcc atttgcaaat cttgcgagac
     1561 gagagcttca cgcctcgtgc agtgacccga gcgcgcgaga cgttacggcg aatttttacg
     1621 cgaggtcgag ctcgccgagc agccggcgcc cgttctcgta cgtggcccag aggcgagtca
     1681 cggttcgctt ggacgccgct acccgcgcca gttcggcctc acaacaggcg tagatcaagc
     1741 cctcggcggg gtcgaaagcg atgtcgggca tgcacttcac cagcttgcca tccggcgtga
     1801 gaacctcgac atcgagcgct ttgacgccgg ccaagtcgag gcggacgcgc gtgatgaaga
     1861 ggtccgcgtc ggccggaatt tctggcgggc gtgggtcggt cgggtcgaga tcgtgaacga
     1921 ctacgcgcaa cccggaggac tgcgccgcgg cgacctcggc tgcggtcatc caaagcgaaa
     1981 tcgtgccgcg cgcactcatc tctcggaagc ccgcggcgag gttcgaccgg aactgaagct
     2041 ccggggcg
//
LOCUS       DASTKS010000923         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1935304, whole genome shotgun sequence.
ACCESSION   DASTKS010000923 DASTKS010000000
VERSION     DASTKS010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935304"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFQ35_19335"
     CDS             <1..1090
                     /locus_tag="VFQ35_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932870.1"
                     /translation="VVKHSGNEGISMINDVINSNIVGNFITDIAGSGITIGHPQHVYL
                     GDGGTHARYPKGVEGIPTKITIDNNVVFQVGTQRGFGSHAGVTAFFTDTLTFTHNHIQ
                     SVAYNGINLGWGWRNFKDSTTCKNNALNNNRFIDMMTRLHDSGAVYTLGQMQGTVINQ
                     NYVRGIPNNSSGPTYGLHNDEGTAYITENDNVLDIDKGVTYTINCEDFGDKHDLTILR
                     TYATVNKMGKNPPASTIDTPVVVSDAVWPVAQYGFAVNSGIEDKYRSIMPSRVLAGAD
                     YVFPASCAAPKNTSALPIRSSGDASNSVWFAPAGTTTFSEGATMTKAGGTSTSIAVPS
                     TAGSYKLFVVDSQGKKLGESAATLRVGS"
     gene            complement(1139..1777)
                     /locus_tag="VFQ35_19340"
     CDS             complement(1139..1777)
                     /locus_tag="VFQ35_19340"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HET9932871.1"
                     /translation="MIYRAFLGATLCMLVGSGCALTSKATPMSPRYFSPEQVPDSRAP
                     SSPRSASKAAAPALQLGQVASASHLDERIAYRVGATELGFYDDRRWTELPEAYLRRAL
                     ERELFEDRGLERMITGGGPTLDVELIAFEELRGAPTRARVTLHFSLHDERRSFLERSV
                     DIEREVTIASGADPAVRLAETLSTTLRDAVRAVAEQVTAELPTATRVAPTPD"
     gene            complement(1774..2733)
                     /locus_tag="VFQ35_19345"
     CDS             complement(1774..2733)
                     /locus_tag="VFQ35_19345"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9932872.1"
                     /translation="MAVATNRWKLGLFVIVGFAVALSALVVFGAQSFSKKTVEVVSFF
                     DESVQGLDVGSPVKFRGVTVGRVSSIDIAENQRHVQVTSALSAEQVGRLKIGARGNVG
                     HSVLHTDMRVQLAQAGITGVKFILIDFVDSKTNPVIPLPFKPPPNYIPTAPSTMKNIE
                     DSVVKTTNRFPEIADDAARAILQIRSVAEQVEQGRLPERAGDTLAQANASLGELNRQL
                     AALNTGQLSRDASKNLQEFNAILVRTNALLSRLDGEQGLFHNAERSADAIRDLAHNAR
                     SVGPELELTLREIRGAARSIKRLADELERDPDMLLKGRAARKP"
     gene            complement(2724..3473)
                     /locus_tag="VFQ35_19350"
     CDS             complement(2724..3473)
                     /locus_tag="VFQ35_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9932873.1"
                     /translation="MTTPLISARELTIGHGETVLLESASFDVARGDIFIILGGSGCGK
                     STLLRHLVGLETPLSGDIVISGVGAPRLEGLAPRYGVMFQSGALFSSLTVGENVGFAL
                     HEWTELPAEAILAIVRAKLALVGLEGAEDKLPAELSGGMRKRAAIARAMAFEPDLIFL
                     DEPSAGLDPISAAELDELILALNRGLGLTVVMVTHELESIFKVGKSCIMLDRTTRSII
                     ARGDPRRLRAESDDPRVRRFFNRETRVPQWQ"
BASE COUNT          695 a         1139 c         1184 g          624 t
ORIGIN      
        1 cgtcgtcaag cacagcggca acgaaggcat ctcgatgatc aacgacgtga tcaactcgaa
       61 catcgtcggc aacttcatca ccgacatcgc cggcagcggc atcacgatcg ggcaccccca
      121 acacgtctac ctgggcgacg gaggaaccca cgcgaggtat ccgaaaggcg tagaaggtat
      181 ccccaccaag atcaccatcg acaacaacgt ggtattccag gtcggcacgc agcgcgggtt
      241 cggctcgcac gccggcgtta ccgcgttttt cacggatacg ctgacgttca cgcacaatca
      301 catccaatcg gtcgcgtaca acggtatcaa cctgggttgg gggtggcgaa acttcaagga
      361 ctcgacgacc tgcaagaaca acgcgctcaa caacaatcgt ttcatcgaca tgatgacccg
      421 gctacacgat agcggcgccg tctacacgct cggtcaaatg cagggaacgg tcatcaacca
      481 aaactacgtt cgagggatcc cgaacaactc gagcggcccg acctacggcc tgcacaacga
      541 cgaaggcacc gcgtacatca ccgagaacga caacgtcctc gatatcgaca agggtgtcac
      601 ctacacgatc aactgcgagg actttggcga caagcacgat ctaacgattt tgcgaacgta
      661 cgcgaccgtc aacaaaatgg gtaagaaccc gcccgccagc acgatcgaca cgccggtggt
      721 cgtctcggac gcggtgtggc cggtcgctca atacgggttt gccgtgaatt cggggatcga
      781 ggacaagtac cggagcatca tgccgagccg cgttcttgcg ggtgccgact acgtcttccc
      841 tgccagctgc gccgccccga agaatacttc cgcgcttccc atccggagta gcggcgacgc
      901 ttcaaattcg gtttggttcg ctccggcggg gaccacgacc ttcagcgagg gcgccaccat
      961 gaccaaggcg ggaggaacgt ccacctctat cgccgtgccc agcaccgcag gaagctacaa
     1021 gctcttcgtc gtcgattcgc aggggaagaa gctcggcgag tccgcggcca cgctcagggt
     1081 cggctcgtaa ttcctgggtc tcaggtgcgc agcccaccga acgacgaacg ttcggtgctc
     1141 agtccggcgt gggcgcaacg cgggtcgcgg tcggcaactc ggcagtcacc tgttcggcga
     1201 ccgcgcggac tgcatcgcgg agcgtggtcg agagggtctc ggcgaggcgg accgccgggt
     1261 cggcgccgct cgcgatggtc acctcgcgct cgatatcgac gcttcgttcg aggaacgagc
     1321 ggcgttcgtc gtgcaagctg aagtgcagcg tcacgcgcgc tctcgtcggt gcaccgcgta
     1381 attcttcgaa ggcgatgagt tcgacgtcga gggtggggcc gcctccggtg atcatgcgtt
     1441 cgagcccgcg gtcttcgaac agttcgcgct cgagcgctcg ccgcaggtag gcctcgggca
     1501 gctccgtcca acggcgatcg tcgtagaagc cgagttcggt cgctcccacg cggtaagcga
     1561 tgcgctcgtc gaggtgcgat gcggatgcca cctgaccgag ctgcagcgcg ggggcggcgg
     1621 ccttggacgc ggaacgaggg ctcgacggcg cacgactgtc cggaacctgt tcggggctga
     1681 agtagcgcgg cgacatcggc gtcgctttgg aagtgagggc gcaaccgctt ccgacgagca
     1741 tgcaaagggt cgcccccaag aacgcgcggt agatcatggt ttcctcgccg ctctgccctt
     1801 gaggagcatg tcggggtcac gctcgagctc gtcggcgagg cgtttgatcg agcgcgcggc
     1861 cccgcggatt tctcggaggg tgagctcgag ttcggggccc acgctgcggg cgttgtgagc
     1921 gaggtcgcgg atggcgtcgg cgctgcgctc cgcgttgtgg aagaggccct gctcgccgtc
     1981 gagccgagag agcagcgcgt tggtcctgac cagaatcgcg ttgaactcct ggaggttttt
     2041 ggaggcgtcg cgagagagct ggcccgtgtt cagcgcggca agttgtcggt tgagctctcc
     2101 gagggaagcg ttggcttgag cgagtgtgtc gccggcgcgc tcggggagcc gaccctgttc
     2161 gacctgctcg gccacgctgc gaatctgcaa gatggcccgc gccgcgtcgt ccgcgatttc
     2221 ggggaaacga ttggtcgttt tcaccaccga atcctcgatg ttcttcatgg tcgagggtgc
     2281 ggtcggaatg tagttcgggg gcggtttgaa gggcagggga atgacggggt tggtcttcga
     2341 atcgacgaaa tcgatcaaga tgaatttcac cccggttatg cccgcttgcg cgagctgcac
     2401 acgcatgtcg gtgtgcaaca cgctgtggcc gacgttgccg cgcgcgccga tcttcaagcg
     2461 gccgacctgc tcggcgctga gcgcgctcgt cacttggacg tggcgctggt tctcggcgat
     2521 gtcgatgctc gagacgcgac ccaccgtgac gcctcgaaac ttcacggggg acccgacgtc
     2581 gaggccttgc acggactcgt cgaagaagct gacgacctcg accgtcttct tgctgaagct
     2641 ttgtgcgccg aatacaacga gtgctgacaa cgcgaccgcg aagcctacga tgacgaagag
     2701 gccgagcttc cagcgattgg tcgctactgc cattggggta ccctcgtttc gcggttgaag
     2761 aatcgtcgaa cgcgagggtc gtcgctctcg gctcggaggc ggcgcgggtc gccgcgcgcg
     2821 atgatgctgc gagtcgtgcg gtcgagcatg atgcagctct tgccgacctt gaaaatgctc
     2881 tcgagctcgt gcgtcaccat gacgacggtg agcccgaggc ctcgattgag cgccagaatc
     2941 agctcgtcga gctcggcggc gctgatcgga tcgagccccg ccgacggctc gtcgaggaag
     3001 atcaagtccg gctcgaacgc catcgcgcga gcgatcgcgg cgcgcttgcg cattccaccc
     3061 gagagctcgg caggaagctt gtcttccgcg ccttcgagcc cgaccaacgc gagcttggcg
     3121 cgcacgatgg ccaggatcgc ttcggcaggc agctccgtcc actcgtgcag ggcaaagccg
     3181 acattttcac cgacggtcaa agagctgaac agcgcgcccg actggaacat cacgccgtag
     3241 cgcggcgcga gcccttcgag tcgcggcgct ccgacgccgc tgatgacgat gtctccggag
     3301 agcggcgtct cgaggccgac caggtggcgg agcaacgtcg acttcccgca gccgcttcca
     3361 cccaaaatga tgaagatgtc accacgcgcg acgtcgaagc tcgccgactc gagcagtacc
     3421 gtctcgccgt ggccaatcgt gagctcacgc gccgaaatga gcggagtcgt cataagccca
     3481 agactcccaa gagcacagtg aagatcgcgt cgagcacgat cagggagaag aggatggaga
     3541 ctaccgcgcc ggtcgtgcgg cgaccgaccc cctccgcgcc gcccgacgtc gaaaggcctt
     3601 gctgacacga gatcaaggcg acggcaccgc tgaaaaccat gc
//
LOCUS       DASTKS010000924         6444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_594098, whole genome shotgun sequence.
ACCESSION   DASTKS010000924 DASTKS010000000
VERSION     DASTKS010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594098"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="VFQ35_19355"
     CDS             complement(<1..1747)
                     /locus_tag="VFQ35_19355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9932874.1"
                     /translation="MHLRPSPATSQSLGGAPGSRIRAWLNGIVEVLGYAGRTLRLVAD
                     SSRTLLSLQVAFTTSAALLPLVVAYVGKRIIDAVVAADSERTLKWVLLELAAVLALTG
                     TQRALGLIRQLLSARMGADLNAAILEKALGLDLTFFENAEFYDKLTRARREASSRPLA
                     LVSDCFQLVQNVVTLLGYSVFLTAFGAWVLPVLFVATIPATFAEMHYSKLVFRVRNWR
                     SPDTRRLLYLEHVLANDEHAKEVKLFGIGPLLLRRYKDLAQGFYLEDKRLALRRAGLT
                     HVLSVLGVGAFYATYASMAFAAARGNISVGALTLYVVSLRQGQQAFQSVLSGIGNIYE
                     HNLYMSNLFSYLSLPSSAQQSEAATPRTALARAGASNGDLGPIAKLGAIRFEDVGFRY
                     PNAEGWALRHVDLEIAPGESVALVGENGAGKTTFVKLLTGLYKPTEGRVSIDGRDVAS
                     YPEEELRQRFAVVFQDFNQYQFRVRENVGLGSVQHLEDVARIARAVERGGASELVQSL
                     PGGLEQPLGHWFEKGSELSGGQWQKLALARAFMREEASILILDEPTAALDAKAEHAVF
                     QHFRELSRGRTSIIIS"
     gene            2129..4894
                     /gene="ppc"
                     /locus_tag="VFQ35_19360"
     CDS             2129..4894
                     /gene="ppc"
                     /locus_tag="VFQ35_19360"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET9932875.1"
                     /translation="MNSIEVRAEDRPLHDDMRYLTTVLGRVIRRLEGEEVFRAVEELR
                     TLSRDRRRASGGAEALDELLERVDALPLDKAAPVARAFTLFFFLINTAEQVHRVRRRL
                     TYEKSGDTTAQPASFAWAFEQLKERGASAHEVREMMRNLDVRPVLTAHPTEATRRTLL
                     GLQARLAQALLSRDDAPLSDRLRAEEALEADVELLWLTDEVRRDRPSVLDEVSSVIWY
                     LEDRLMEAAGRASAAAERAFRGVFGKDLGFSTQIGMGSWVGGDRDGNPFVTPEITLAA
                     TRRTAYALLARYRAYVEKLVGHVSISDRIAPATEDLRASLDRDKALLPAIWEANRRRD
                     AHEPLRLKLTFISGRIEALRKEIAARDAGSSVPVAGAYKDHNELIADLELVRQALVLA
                     GAEHTRRTQLEPFIAQVKAFGFFGYTLDLREDSDAHAQAIQKVYAALGMPALDKDGLR
                     QELLGRRPLVADHLPLDENTKKVLDVFRAMRTVQDELGERAAPTYIVSMTHHAEDLLN
                     VLLLAREAGLVDLSNDEPMSRVDVVPLFETRDDLARGPSIMKSLFDDPVYKRQLRARR
                     MRQEVMLGYSDSAKDVGMLAASWELYLAQERLAALAKEYGVELTLFHGRGGTVGRGGG
                     SPVFRALSALPPGTVGGRIKITEQGEVISQKFGLLPLAERSLEVMATGTLLAAFNDFS
                     QSLDPELLKRFRAAIEEMSETSRIAFRRIIHEDPSLFQFFLTATPVRELTHVHFGSRP
                     AYRERGAGSIVGIRAIPWNFGWTQMRWMASAWVGAGTALEAALDKPGGVELLRAMAEK
                     WPFFDDMLDKLEMVVAKADPDVARMYARVLGADARVMSGLEAEFERTLKSLHRIRGRE
                     LIAEHRFLRDSLNLRNPYVDPLSLLQVSLLRKKRALPEGHPDLRHIDSALGTTLNGIA
                     QGMRNTG"
     gene            5283..>6444
                     /locus_tag="VFQ35_19365"
     CDS             5283..>6444
                     /locus_tag="VFQ35_19365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932876.1"
                     /translation="MAMPETSQESGLLKQGRYRIVGLLGRGAQADTLWAEDAHTGTQV
                     AIKRFQVRGAKSWKDVELAEREASVLSRLSHKNLPRYIDHFEEDGALYLVMEKIEGKT
                     LAQLRERGALDQASVRRLLAELGETLEYLHGLSPPIVHRDIKPQNVILRGDGGFSLVD
                     FGSVRDKLRPEGGSTVVGTFGYMAPEQFQGRAGPSTDVYGAGATALACLTGSDPENLP
                     HKGLSIDVPAALAGHVDASMVQLLSRLVEPDPDKRPRSLTAALREAAPARRSAPPRSN
                     AEAASRADRIPPFDVPSMRLSRRQLRRLEKERRKEAKRARRSARRAQRLERLGNRVAH
                     FPGWFWVLLVLGITLFVVRIVLGGLLRIALPILLSVLAIVFGPALRRAVGQSL"
BASE COUNT         1147 a         2183 c         2039 g         1075 t
ORIGIN      
        1 gagagatgat gatgctcgtg cgaccgcgcg acaactcgcg aaagtgctgg aacacggcgt
       61 gctccgcctt cgcgtcgagg gcagcggtcg gttcgtccaa gatcaagatg ctcgcctctt
      121 cgcgcatgaa cgcgcgcgcc aacgcgagct tttgccattg tcctcccgag agctcgctgc
      181 ccttctcgaa ccaatgcccg agcggctgtt ccaagcctcc tggcaacgac tgcacgagct
      241 cgctcgcgcc acctcgctcc acggcgcgcg cgatgcgggc cacgtcttcc aggtgctgga
      301 cgctcccgag ccccacattt tcgcgcactc tgaactggta ctgattgaaa tcctgaaaga
      361 cgaccgcaaa acgctggcgc agctcttctt ctggatacga cgccacgtcg cgcccatcga
      421 tgctcactcg cccctcggtc ggcttgtaga gtccggtgag cagcttcacg aaggtcgtct
      481 tgcccgcacc gttttcgccg accagcgcga cgctctctcc cggtgcgatc tcgaggtcga
      541 cgtgccgcaa cgcccagccc tcggcgttcg gatagcgaaa gcctacgtct tcgaagcgaa
      601 tcgcacccag tttggcgatg gggccaaggt caccgttcga cgcgcctgcg cgggcgagcg
      661 ccgtccgcgg cgtcgcggct tcgctctgtt gcgccgagct cggcagcgac aggtacgaga
      721 acaggttcga catgtagagg ttgtgctcgt agatgttgcc aattcccgag agcacggact
      781 gaaacgcctg ctgcccctgg cgcagcgaga ccacgtacag cgtgagcgcg ccaacgctga
      841 tgttccctcg cgccgccgcg aacgccatgc tggcgtacgt cgcgtaaaac gctccgacgc
      901 ccagcacgct gagcacgtga gtcagccccg cgcgtcgcag cgcgagccgc ttgtcctcga
      961 ggtaaaaccc ctgcgcgagg tctttgtatc gccgcaagag caatggcccg atgccgaata
     1021 gcttcacctc cttggcgtgc tcgtcgttcg ccagaacgtg ctcgagatag agcagccgac
     1081 gcgtatccgg cgaccgccag ttgcgtacgc gaaacaccag tttcgagtag tgcatttccg
     1141 caaaggtcgc cgggatcgtc gcgacgaaca gcaccggcag gacccacgcg ccaaacgcgg
     1201 tcaagaacac cgaatagccg agcagggtga cgacgttttg aacgagctga aagcagtcgc
     1261 tcacgagtgc gagcggacgc gacgacgctt cgcgccgtgc ccgcgtgagc ttgtcgtaaa
     1321 attcggcatt ttcgaagaac gtgaggtcca ggcccagcgc cttctcgaga attgcagcgt
     1381 tgaggtcggc gcccattcgc gccgacaaga gctgccgaat caaacctagc gcccgctgtg
     1441 tccccgtgag cgcgagcacg gccgcgagct cgagcagcac ccactttaaa gtgcgctcgg
     1501 aatctgccgc gacgaccgca tcgatgattc gcttgccgac gtaggcgacg acgagcggca
     1561 aaagcgccgc gctcgtcgta aacgccacct gtaaagacag aagcgtacga gatgagtcgg
     1621 caacgagccg gagcgtgcgg cccgcgtagc ccagcacctc gacgattccg ttgagccacg
     1681 cccggatgcg gctcccggga gcgcctccca gcgattggct cgtggcaggg ctcggccgca
     1741 agtgcatcgg tcgtctttag ccgagcggca cttcgctttc gacctgcccg ttcggcggac
     1801 caggaggcgc cccaagttat ggcccgtcgc gagagctaag aaaaggcgag cgagaagcgc
     1861 gtgcgaggcg cgagcaccga caaaaaagcc agaccacggg cgaacgagcg agctcgctaa
     1921 gcgtcgggcc gcgacgagtc gacccccggg ccattggccg ccgcccgcag cgggcccggg
     1981 ctcggtgagg cgagctagcg cgattcgctc gcagagaatt gatcccgctg aaaacagtcc
     2041 ggaaacggag tgcgcgtcag cctactggcc ttcgcctggt gtccgtgaca gatcgcccat
     2101 ctcgcctttc tcgcccatcg aggagcccgt gaacagcatc gaagtccgag ccgaagaccg
     2161 tcccctccac gacgatatgc gttacctgac cacggtgctg ggtcgcgtca tccggcgact
     2221 cgagggagag gaggtatttc gtgcagtcga ggaactgcgc accttgagtc gcgaccgccg
     2281 acgcgcttcc gggggcgccg aggccctcga cgagctgctg gagcgagtcg acgccctgcc
     2341 cctcgacaag gccgcaccgg tcgcgcgcgc gttcacgctg ttcttcttcc tcatcaatac
     2401 ggccgagcag gtgcaccgag tgcgccgtcg gctcacgtac gaaaagagcg gcgacacgac
     2461 cgcccagccg gcgagctttg cttgggcttt cgagcagctc aaagagcgcg gcgccagcgc
     2521 gcacgaggtg cgcgaaatga tgcgaaacct cgacgtgcga ccggtgttga ccgcgcaccc
     2581 gaccgaagcg actcgccgaa cgctgctcgg cttgcaggca cgcctggcgc aagcgctctt
     2641 gagccgcgac gacgctccgc tctcggatcg tttgcgcgcc gaggaggcgc tcgaagccga
     2701 tgtcgagctc ttgtggctca cggacgaagt gcggcgcgac cgcccgagcg tcctcgacga
     2761 agtcagctcc gtgatctggt acctggaaga ccgcctgatg gaggcggccg gccgcgcgag
     2821 cgccgccgcc gagcgcgcgt tccggggcgt attcgggaaa gacctgggct tcagcactca
     2881 aatcgggatg ggcagttggg tgggcggtga tcgcgacggc aacccgttcg ttacccccga
     2941 gatcaccctg gccgcgacgc gacgcaccgc gtacgcgctg ctggctcgct atcgcgcgta
     3001 cgtcgaaaag ctcgtcgggc acgtctcgat ttcggaccgc atcgcgcccg ccacggaaga
     3061 tctgcgcgca tcgctcgatc gcgacaaggc gctgctgccg gcgatttggg aggcaaaccg
     3121 gcgccgcgac gcgcacgagc cgctgcggct gaaactcacc ttcatctccg ggcgcatcga
     3181 agcgctccgc aaagagatcg ccgcgcgcga cgcgggctct tctgtcccgg ttgcgggcgc
     3241 ttacaaggac cacaacgagc tcatcgcgga cctcgagctc gttcgacagg ctctggtgtt
     3301 ggcaggggcg gagcacactc gtcgcacgca gctcgagccg ttcatcgcgc aggtcaaggc
     3361 cttcggcttc ttcgggtaca cgctcgactt gcgcgaagat tcggacgcgc atgcgcaggc
     3421 gatccagaag gtctacgccg cgctcggcat gccggcgctc gacaaagacg gcctgcgaca
     3481 agagctgctc ggccgccgtc cgctcgtcgc cgaccacttg ccgctcgacg aaaacacgaa
     3541 gaaggtgctc gacgtcttcc gcgccatgcg caccgtgcag gacgagctcg gagagcgcgc
     3601 cgcgcccacg tacatcgttt cgatgacgca ccacgccgag gacttgctca acgtgctgct
     3661 cctcgcgcgc gaagcgggcc tcgtcgacct cagcaacgac gagccgatgt cgcgcgtcga
     3721 cgtcgtgccc ctcttcgaga cacgagacga tctcgcgcgc gggccttcca tcatgaagag
     3781 cctgttcgac gacccggtgt acaagcgcca gcttcgtgct cggcgcatgc gccaagaggt
     3841 aatgctcggc tactccgact cggccaagga cgtcggaatg ctcgcggcgt cttgggagct
     3901 ctatctcgct caagagcggc tcgccgccct cgccaaggag tacggcgtcg agctcaccct
     3961 gttccacgga cgcggcggca ccgtcggtcg tggcggcggc tctcccgtat ttcgcgccct
     4021 ttccgcgttg ccgccgggta cggtcggcgg gcgcatcaag atcaccgaac agggcgaggt
     4081 catttcgcag aagttcggcc tcttgccgct cgcggagcgc agcctcgagg tgatggcgac
     4141 ggggacactg ctggccgctt tcaacgactt cagccagagc ctcgacccgg agcttttgaa
     4201 gcgcttccgc gcggccatcg aggagatgag cgagacctca cgcatcgcgt tccgccgcat
     4261 catccacgaa gatccctcgc tctttcagtt cttcctcacc gcgacgccag ttcgcgagct
     4321 cacccacgtg cacttcggtt cgcggcccgc ctatcgcgag cgcggcgccg gcagcatcgt
     4381 gggcatccgc gcgatcccct ggaacttcgg ctggacgcaa atgcgctgga tggcttcggc
     4441 ctgggtcggc gccggcacgg ctctcgaagc cgcgctcgac aaacccggcg gcgtcgagct
     4501 cctgcgcgcc atggccgaga agtggccgtt cttcgacgac atgctcgaca agctcgagat
     4561 ggtcgtcgcc aaggccgacc ccgatgtcgc ccgcatgtac gcgcgcgtcc tcggcgcaga
     4621 tgcgcgcgtg atgtccggcc tcgaggcgga gttcgagcgc accctcaaga gcctgcaccg
     4681 cattcgcggg cgcgagctca ttgcggagca ccgctttttg cgagattcgt tgaacctcag
     4741 aaatccgtac gtcgatccgt tgtcattgct ccaggtcagc ctgcttcgaa agaagcgcgc
     4801 gctgccggaa ggtcaccccg atcttcggca catcgactcg gcactcggca cgaccttgaa
     4861 cggcatcgcg caggggatgc gcaataccgg ctagttcgcc gagcctctcc tccacggggg
     4921 ccttgggttc gccccggccg caaaacgcgg atttgcttgg cgtgcgagcg ccgacgacca
     4981 tcggagccag cgtgacccgt tcttggtcgg tgctttcgta cgcctaacgc aaaggggagc
     5041 tcgtagcccg ggcttcggcg cccgagcgca cccccctcgc cttgcgtgag gaaatagttc
     5101 gcatgctcct ttttgtattt tggggccggc aaaccccaag acggtgctcc gaagcgtggg
     5161 ttggagcacg ttgaatgatc atgaagaatc cgagcgcccc tgccgttttg cgattcgagg
     5221 cgtatgaaga gggacggccg tccacgaccg ttcgctgcag ggtggctgaa cgagaccgaa
     5281 gcatggcgat gcccgaaacc tctcaagagt cagggctgct caaacaaggc cgctaccgca
     5341 tcgtcggtct attggggcga ggcgcgcaag ctgacacgct ctgggccgaa gacgcgcaca
     5401 ccggcacgca agtcgccatc aagcgctttc aggtgcgcgg cgccaaatcg tggaaagacg
     5461 tcgagctcgc cgagcgcgaa gcgagcgtgc tctcccgcct cagccacaag aacctcccgc
     5521 gctacatcga ccacttcgag gaagacggcg cgctctacct cgtcatggaa aagatcgagg
     5581 gcaagacctt ggcgcagctc agggagcgcg gcgctctcga ccaagccagc gtacgtcgat
     5641 tgctagctga gctcggggaa acgctcgagt acctgcacgg cctgtcgcct ccgatcgttc
     5701 atcgcgacat caagccgcag aacgtgatcc tacgaggcga cggcgggttt tccctcgtcg
     5761 acttcggttc ggtgcgcgac aaattgcggc cagagggcgg aagcaccgtg gtcggcacct
     5821 tcgggtacat ggcgccggag cagttccaag gtcgtgctgg accgagcacc gatgtttacg
     5881 gcgccggagc gaccgcgctc gcttgtctca ccggcagcga cccggaaaac ctgccgcaca
     5941 agggcctttc catcgacgtg ccggcggcgc tagctggcca cgtcgacgcg agcatggttc
     6001 agcttctgtc acggctcgtc gaaccggacc ctgacaagcg tccgcgctcg ctcaccgcgg
     6061 ccctgcgaga agccgcgccc gcccgccgca gcgctccgcc gcggagcaac gccgaagcgg
     6121 cgtcgcgagc ggaccgcatt cctcccttcg acgtcccgag catgcgtcta tcgaggaggc
     6181 agcttcgccg cctcgaaaag gaacgccgaa aggaagccaa gcgagcgcgc cggtctgcac
     6241 ggcgggcaca acgactcgag cgcttgggga atcgcgtcgc ccacttcccc ggctggttct
     6301 gggtgctgct cgtactcgga atcaccttgt tcgtcgtgcg tatcgtcctc ggtggcctgc
     6361 ttcggatcgc gttaccaatc ctattgagtg tgctcgccat cgtgttcggt cctgcgctac
     6421 gacgcgccgt cgggcaatcg ctgc
//
LOCUS       DASTKS010000925         5708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1711208, whole genome shotgun sequence.
ACCESSION   DASTKS010000925 DASTKS010000000
VERSION     DASTKS010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711208"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="VFQ35_19370"
     CDS             <1..1649
                     /locus_tag="VFQ35_19370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9932877.1"
                     /translation="LARTGSCSLGRVLDTLIVGGGLSGLALASLLKAKKIDCRVYEAR
                     ERLGGRVSTLREPNTIVTDARGLTRELPGRRLDCGATWFWPADNPLLSRLLDALGIAT
                     FEQYETGRLVLASAKGRPRRATSNPAYASARRIVGGAGAAIEGLAARVGEERIRLGHE
                     LLNVVRHDDYIELGFRYRGEVVNVRARRVALAMPPRLLHAAVDFSPKLSVEVQSALAA
                     TPTWMASRAKALARFPEAFWRRNGDSGAALASYPDAVLGEVFDASDANGRPALGGFFA
                     LGPHERRARAEELPELIRAQLVELFGPLPDDAPASHIQDWACERFTATLLDLEQPASE
                     PTAAPHALREPLWERRLFIAGTETGNHHAGHMEGALEAALRVASLLREPVEVSDPKGR
                     ARLDPATEAWLEHFHTSARARRSAAADVYRDEIARSLSAQDYGDMTQRALLEAARSAY
                     RGALKELAPAPFLSGTREPSASAELVEALVSAFAGFSQELIAVALKFNQTSCAMRAFP
                     DEARPGGEYLVVLRNDLRSLFEEFERQIGLCVRVETTSES"
     gene            1775..2944
                     /locus_tag="VFQ35_19375"
     CDS             1775..2944
                     /locus_tag="VFQ35_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932878.1"
                     /translation="MTRPRIETRRRRAQGRALLSALLLFGVATSATALPNAEAPMPKK
                     RAAVLSVRGVESEPKDRKRPTTDELALLRSNVEANPKLRKPRLELVRALMSSSDLRGA
                     HDAAAAWREHDAYNLVVVRLLGDIESELGDKASARRTYSAIVELLPRDVEARRALSTV
                     LKQAGDLEGARAQLLAALGMEKSDPRIVFELGDVEQRLGLEESARARFESTKDAADAS
                     EALRYPARQRLAQIHARALRDAERRGDRNTVGELTHRIEELGIHGGTENDVKVYLSWD
                     SDRTDVDLWVTTPAGEQVYYAHRNGSGGEALFDDVTTGYGPESFTAPHAQVGDYVVQV
                     KYFGSGGGALKDARGEVMVVLNEGRPSEVRQVFPYRLFEVNDVVSVARIHVEQGS"
     gene            2941..5235
                     /locus_tag="VFQ35_19380"
     CDS             2941..5235
                     /locus_tag="VFQ35_19380"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HET9932879.1"
                     /translation="MKTLGIGVLLSALAAVGCGKTAQTPSGPNAPVTSALEAPRDSGG
                     FGWLSARGARGEPGAVELTRVHVSAKQVGDYAALSIDHVFQSQSDAQLEGVFRFPLPE
                     GALLTGLAMLIDGKLVEGELVEREKARKAYEQIVDSMQDPALLEWEHGSTFKMRVFPI
                     VPHEQKRVTVRYLVPLTHSAKGWVFSQSTRRGSSSEPVPELRIDWEGKPVFQERDVHP
                     GRVIETPAADPAIVLREQRDGSVYTAVRVHPDWSKVPVEPREKPRHVVFALDTSRSSL
                     EESKLSLEALRAALSALTPDQSFVVVTHDLDARIDPHGFMPAEKSSIEAALTFANQAQ
                     PDGATDLGALFDTIARIAHGFTDVSVVYIGDCEPSWGILDQQALVERARGALGKTAFN
                     PIVIGSATDAELAQRLAQASGGRWLRAEHAADATGFVRRLSDSVARLPEVEVSAAPEA
                     EVFPSGKLSLEAGEELTLLVKTAPGKEALDLVRVRARVAGKEQSLLPKATPTTANGVA
                     QRFGAELIRSLERQAKPREDVIEASLSYGVMSKYTSFLVLESEEAYARFAIERKNRQE
                     AEAPRVSGADLASLDGDGAQVSLDRIQPGDPEISIDAPRDCQSVTVVFPFGEQKTAVF
                     DTEANHGRGAWMVRFLVDRDTPEGRYEVLAFIVYRDGHRELRKVSYTVDRSAPELKVD
                     LKRAPRRPGWYEVHVTQDAPESERDLRRVEVRTPEGQSIALTPIRWAEFRGYVKIPAR
                     RGSILRVAGFDQALNHRSIEVQAP"
     gene            5232..>5708
                     /locus_tag="VFQ35_19385"
     CDS             5232..>5708
                     /locus_tag="VFQ35_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932880.1"
                     /translation="MKRLFGCTLVAALFANAPLHAEPSRILGTDDARDCALANDGTVV
                     GATAGGLVVFGAKRGAKVLTALEGLPDTRVESVRIEGGYAEIQTKSGASRVRLGDLQV
                     EPIGGELTPRPPSMPSDPFRARAEQRSAEGRCLATDHGVFFATGTKGLERVPLAGLP"
BASE COUNT          940 a         1880 c         1935 g          953 t
ORIGIN      
        1 cgctcgcacg gaccggatct tgctccttgg gtcgcgtgct ggatacgctg atcgtcggtg
       61 gtggcctatc ggggctcgcg ctcgcgagtc ttctcaaagc caaaaagatc gactgccgcg
      121 tctacgaggc gcgcgagcgg cttggcgggc gcgtgtcgac cctcagggag ccgaacacga
      181 tcgtcacgga cgcgcggggg ctcacgcgcg agcttcccgg gcgcaggctc gactgtggag
      241 cgacctggtt ttggcccgct gacaatccgc tgctgagccg tttgctcgac gcgctcggca
      301 tcgccacttt cgaacagtac gagacggggc gtctcgtgct cgcgtcggcg aaaggccgtc
      361 cgcggcgtgc aacttcgaat ccagcttatg cttcggcacg acgcatcgtc gggggcgcgg
      421 gcgcggcgat cgagggcctt gcggcgcgcg ttggcgaaga gcgcattcgc ctcggtcacg
      481 agctcctcaa cgtcgttcgg cacgacgact acatcgagct cggctttcgc taccgcggtg
      541 aggtcgtgaa cgtgcgggcg cgcagggtag cgctggcgat gcccccgcgc ttgctgcacg
      601 cggcggtgga tttttccccg aagctgtcag tcgaggtgca gtcggcgctc gcggcgacgc
      661 ccacttggat ggcttcacgt gccaaggcgc tcgcgcgatt tccggaagct ttttggcgcc
      721 gcaacgggga ctcgggcgcc gccctcgcat cgtacccgga tgcggtgctc ggtgaagtgt
      781 tcgacgcgag tgacgccaat ggccgtcctg cgctcggagg cttcttcgcg ctcggccctc
      841 acgagcgcag ggcgagagcc gaagagctcc ccgaactgat tcgagcgcag ctcgtcgagc
      901 tctttggtcc cctcccggat gacgcccccg cgtcgcacat ccaggactgg gcgtgcgaac
      961 gctttaccgc gactctgctc gatctcgagc agcccgcgag cgagcccacc gccgcgcctc
     1021 acgccttgcg tgagccgctc tgggagcggc gtctcttcat cgccggaacg gagacgggta
     1081 accatcacgc cggtcacatg gaaggtgcgc tcgaggccgc cctgagagtc gcatcgctac
     1141 tccgcgagcc cgtcgaagtc tccgatccca agggccgcgc acggctagat cctgcgaccg
     1201 aggcttggct cgaacacttt cacaccagtg cgcgagcgcg tcgttcggcg gctgctgacg
     1261 tgtatcgcga tgagatcgct cgctctctgt ctgcgcaaga ttacggcgac atgactcagc
     1321 gggccctgct cgaggcggcc cgctccgcct accgcggcgc attgaaggag ctcgcgccag
     1381 ccccgtttct gagcggaacg cgggagccga gcgcaagcgc ggagctcgtc gaggctctcg
     1441 tctcggcctt cgccgggttc agtcaggagc tcatcgcggt cgccctcaag ttcaaccaga
     1501 cttcctgtgc catgcgggcg ttccctgacg aagcacgacc gggcggcgaa tacctggtcg
     1561 tcctccgcaa cgacttgcgc agcctcttcg aggaattcga gcggcagatt ggtctttgcg
     1621 tgcgggtgga gaccacttcg gagtcctgag gctcccgctc ggcttcgcgt gtctcgagat
     1681 ccgcgtctcg cgtctcgacg gacacagggc tcggcatggt gcagccaaaa atggctggct
     1741 tctcggggca cgcgccttgc gatgcccccg ccgcatgacg agaccgcgta tcgaaacgag
     1801 gcgccgaagg gcccagggca gggcgttgct ctcggcgctg cttttgttcg gcgttgccac
     1861 cagcgccacc gcgcttccca acgccgaggc gccgatgccg aagaagcgag ccgccgtcct
     1921 ctccgtgcgg ggagtcgaga gcgagccgaa ggatcgcaag cgcccaacca ccgacgagct
     1981 cgctttgctc cgctcgaacg tcgaagcaaa cccaaagctc cgaaagccgc gcctcgagct
     2041 cgttcgggcg ttgatgtcga gctccgactt gcgcggggct cacgacgccg ccgcggcttg
     2101 gcgcgaacac gacgcctaca acctcgtcgt cgtgcgactt ctgggtgaca tcgaaagcga
     2161 gctcggagac aaggcgagcg cgcgccgcac ctattcggca atcgtggagc tcttgccgcg
     2221 cgacgtcgag gcgcgtcgcg ctctctcgac ggtgctgaag caagccggcg acttggaggg
     2281 cgctcgcgcg cagctgctcg ccgcgctggg catggagaag agcgacccgc gcatcgtgtt
     2341 cgagctcggc gacgtcgagc aacgcctcgg cctcgaggag tcggcgcggg ctcgcttcga
     2401 aagcacgaag gacgccgccg acgcgagcga agcgctgcgc tacccagctc ggcagcgcct
     2461 cgcgcagatt catgctcgag cgctgcgcga cgccgagcgg cgcggtgatc ggaacaccgt
     2521 cggcgagctt actcaccgca tcgaagagct cggaattcac ggcgggaccg agaacgacgt
     2581 gaaggtttat ctctcctggg acagcgaccg caccgacgtc gatttgtggg tcacgacccc
     2641 agccggtgag caggtctatt acgcgcaccg aaacggcagc gggggcgagg cgctcttcga
     2701 tgacgtgacc acgggctacg gccccgagag cttcaccgcg ccgcacgcgc aggtgggcga
     2761 ctacgtcgtt caggtgaagt acttcggcag cggcggcggc gcgctgaaag atgcgcgcgg
     2821 cgaggtgatg gtggtgttga acgaagggcg cccgagcgaa gtgcgccagg tgtttccgta
     2881 tcgactgttc gaggtcaacg acgtcgtcag cgtcgcgcgc atccacgtgg agcaggggtc
     2941 atgaagacgc tcggtattgg tgttctcttg tccgccctcg cagccgttgg gtgcggcaag
     3001 acggcgcaaa ctccgtctgg gccgaacgct cccgtgacca gcgctctcga ggcgccgcgt
     3061 gactccggcg gcttcgggtg gctcagcgct cgaggtgcgc gcggcgagcc cggcgcggtc
     3121 gagctcaccc gcgttcacgt cagcgccaag caggtcggcg actacgccgc gctcagcatc
     3181 gaccacgtct ttcaaagcca atccgacgcg cagctcgagg gcgtattccg gtttccgctg
     3241 ccggagggcg cgctgctcac cggtctcgcg atgttgatcg acggcaagct cgtcgaggga
     3301 gagctggtcg agcgcgagaa ggcccggaaa gcgtacgaac aaatcgtcga ttcgatgcaa
     3361 gatccggcgt tgctcgagtg ggagcacggc tccaccttca aaatgcgggt gttcccgatc
     3421 gtgccccacg agcaaaaacg cgtgacggtt cgttacctcg tcccgctcac gcacagcgcg
     3481 aaggggtggg tattctcgca gagtacgcgt cgcggctcgt cgagcgagcc cgtaccggag
     3541 ctccgcatcg attgggaggg aaagccggtg ttccaggagc gcgacgtgca ccccggtcgc
     3601 gtcatcgaga ctcctgccgc ggacccagcg atcgtgttgc gagagcagcg cgatggctcc
     3661 gtctacaccg cggtgcgcgt gcaccccgac tggagcaagg tgccggtgga gccacgcgag
     3721 aagccccggc acgtggtgtt cgcgctcgat acctcgcgga gctcgctcga agagagcaag
     3781 ctctctctcg aagcgctgcg cgccgcgctc tccgcgctca ctccggacca aagcttcgtc
     3841 gtggtcacgc acgacctcga cgcgcgcatc gatccgcacg gcttcatgcc cgcagaaaaa
     3901 tccagcatcg aggcggcgct cacgttcgcg aaccaggctc agccggacgg cgctacggat
     3961 ctcggtgccc tgttcgatac gatcgcacgc atcgcccacg gcttcacgga cgtgagcgtc
     4021 gtgtacatcg gcgactgcga gccgagttgg ggcattctgg atcagcaagc gctcgtggag
     4081 cgcgctcgcg gtgcactcgg taagacagcg ttcaacccca tcgtgatcgg ttcggcgacc
     4141 gacgccgaac tcgcgcagcg gctcgctcag gcgagcggcg gtcgttggct tcgcgccgag
     4201 catgccgccg acgccaccgg tttcgttcgc cgcttgtccg attcggtagc gcggttgccc
     4261 gaagtagaag tgagcgccgc gccggaggcc gaggtgttcc cgagcggcaa gctctcgctc
     4321 gaagcgggcg aggagctcac cttgctggtc aagaccgcgc cgggcaaaga agcgctcgat
     4381 ctcgttcgcg tgcgcgcgcg tgtcgccgga aaggagcaga gccttcttcc gaaggccacg
     4441 ccgacgacgg caaacggcgt ggcgcagcgc ttcggggcgg agctcattcg ttccctcgag
     4501 cgtcaagcaa agccgcgtga agacgtgatc gaagcctcgc tcagctatgg tgtcatgagc
     4561 aagtacacct cgttcctggt gctcgagagc gaagaggcgt acgcgcgctt cgcgatcgag
     4621 cgcaagaatc ggcaagaggc cgaggcgccc cgcgtgagcg gtgccgatct cgcctcgctc
     4681 gacggcgacg gagctcaggt gagcttggat cggatccaac cgggcgatcc ggagatctcg
     4741 atcgacgctc cgcgcgactg ccagagcgtc acggtggtgt tcccgttcgg cgagcaaaag
     4801 acggcggtgt tcgacaccga agcgaaccac ggtcgcggcg cctggatggt gcgcttcctc
     4861 gtcgatcgag acactcccga gggacgttac gaagtgctcg cgttcatcgt gtaccgcgac
     4921 ggtcaccgag agcttcgaaa ggtgagctat accgtcgacc gttccgcgcc ggagctcaaa
     4981 gtcgacctca agcgcgcgcc gcgccggccc ggttggtacg aagttcacgt tacccaggac
     5041 gcgcccgaga gcgagcgcga tttgcgtcgc gtcgaggtgc ggacgccgga ggggcaatcg
     5101 atcgcgctca cgccgattcg ctgggcagag tttcgcggct acgtgaagat tcccgcgcgt
     5161 cgcggctcaa tccttcgcgt cgctggcttc gaccaggctt tgaaccaccg aagcatcgag
     5221 gtccaggctc catgaaacgc cttttcggct gcacgctcgt tgcggcgctg ttcgcaaatg
     5281 cgccgctcca cgccgagcct tcgcgaattc tgggcaccga cgacgcgcgc gactgtgcgc
     5341 tcgcgaacga tggaaccgta gttggcgcga ccgccggagg tttggtcgta ttcggcgcga
     5401 agcggggggc gaaggtgctg actgccctcg aggggttgcc ggatactcgc gtcgaaagtg
     5461 tccggatcga aggcgggtac gccgaaatcc aaaccaagag cggcgcgtcg cgcgtgcggc
     5521 tcggcgactt gcaagtggag cccattggcg gcgagctcac gccgcgcccg ccttcgatgc
     5581 ccagcgatcc tttcagggct cgcgccgagc agcgaagcgc cgagggtcgt tgtctcgcca
     5641 cggatcacgg cgtgttcttc gcgaccggca ccaagggcct cgagcgcgtg ccactcgccg
     5701 ggctgcct
//
LOCUS       DASTKS010000926         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_817082, whole genome shotgun sequence.
ACCESSION   DASTKS010000926 DASTKS010000000
VERSION     DASTKS010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817082"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /gene="prs"
                     /locus_tag="VFQ35_19390"
     CDS             <1..515
                     /gene="prs"
                     /locus_tag="VFQ35_19390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET9932881.1"
                     /translation="HLYALPVFLEDYLQKRFTADSVFVSPDAGGVERARAYSKRLGAT
                     LAIIDKRRDRPNVSEVMHLIGDVRGRDCIIVDDMIDTAGTLCNAARAAMDYGARRVVA
                     CATHGVLSGPAVQRIEQSPLEEVVVTNSFPLSEEARACKKIRVLSIGRLLGEAIRRIH
                     NSDSVSSLFV"
     gene            547..1221
                     /locus_tag="VFQ35_19395"
     CDS             547..1221
                     /locus_tag="VFQ35_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9932882.1"
                     /translation="MEIIKINASRRESNGKGGARRVRNTGSIPAVAYGKDLAAQALAI
                     APAELSRVLASVHGKNTVLELDVEGSQKITALLREYQHHPVTREYLHADFQQIHLDQE
                     VDVDVTLEVTGKAAGVVLGGTLRQVFRKLPIRCLPEKIPVKISYDVTALGLDGHVAVR
                     DLGLPDGVKVRLPPERTLIAVVKEKQAPEEEAAGGPAAAAPAAAAAGGKAEAGKAGPS
                     KPPEKK"
     gene            1218..1793
                     /gene="pth"
                     /locus_tag="VFQ35_19400"
     CDS             1218..1793
                     /gene="pth"
                     /locus_tag="VFQ35_19400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9932883.1"
                     /translation="MILIVGLGNPGPRYADTRHNAGFQVVDRLVERAGGASFREKFHG
                     HFAQVTVDGAQLSLLKPMTFMNESGRSVQAAVQHFKLPLDSLLVVHDELDLPFAEVRL
                     KQGGGDAGQRGVRSISGALGPNYPRLRVGIGRPPPEFKGDVADFVLQACSLADRDEMR
                     AAIDRAADAVVLVATRGISAAMNATNQRKTR"
     gene            1843..2379
                     /gene="rpsF"
                     /locus_tag="VFQ35_19405"
     CDS             1843..2379
                     /gene="rpsF"
                     /locus_tag="VFQ35_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9932884.1"
                     /translation="MAEATVAVPHRAREYETIYVLKPDVSRESQERIAGRIGEVVSRE
                     SGKLTALENWGRRQLAYTVAKYRRGVYVYVKYLGGGALVSELERNLRMLDDVLKFQTV
                     QTAVDVDFGTVQVAPENVQFESVEPATPEELAEPIERLLGLEQHPERERPSDLDDAEE
                     DYEAAAEAAKAADGGEQS"
     gene            2376..2675
                     /gene="rpsR"
                     /locus_tag="VFQ35_19410"
     CDS             2376..2675
                     /gene="rpsR"
                     /locus_tag="VFQ35_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9932885.1"
                     /translation="MKEMSRGERPSERPDARGGDKDAAGNAFRRVRRRGCAYCSDDAA
                     PIDYKDPQSLKYFVTERGKIVPRRISGTCAKHQRSITLAVKRARNIALLPFTSND"
     gene            2686..3141
                     /gene="rplI"
                     /locus_tag="VFQ35_19415"
     CDS             2686..3141
                     /gene="rplI"
                     /locus_tag="VFQ35_19415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9932886.1"
                     /translation="MATNVKVLLKSEVENLGSGGEVVKVRAGFARNFLLPRGLALPAT
                     AGNLKNVEELKRAAAERAKADREQAEELAKKLGALSVKITRAVGEEGKMYGSVTSKDI
                     ADAVSQTGVTLDRKRIVLPEPLKQLGSFDVAVKLHHDVSTTIKVEVAKQ"
     gene            3475..>4638
                     /locus_tag="VFQ35_19420"
     CDS             3475..>4638
                     /locus_tag="VFQ35_19420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9932887.1"
                     /translation="MSFGQHGNRQAAQDLMQLQPSPGRIPPNDLDAEAAVLSAVLLDS
                     SSFDQVQETLSPDHFYADANRRIFEAVVDLQNSGRPVDIVSVAGYLRDRNRLDQIGGT
                     PYLAQLSDATPAVAHVAAHARAVREKWRLRQLIATCQRFAAEGYATPPDVQKFIDAAE
                     QAVFDIARTPEGTTVEPVRDAVVRAFKILEEAERRGGGVTGVPTGFTRFDKQCSGLHA
                     GDLYIVAARPGMGKTSFVLNLALNVAATRAQKSRSNGEEYFEPEVEEAGYGVLFCSLE
                     MPKEQLAARLLASEAQVDMSKIRSGSMTREDWSRLTEAASRLARLPLWLDDTPALTLL
                     DLRAKIRRLQAEVSRGEGAARAQKLGLVVIDYLQLMQGRKDAGSREQEISELSR"
BASE COUNT          841 a         1474 c         1558 g          765 t
ORIGIN      
        1 atcatctcta cgcgctgccg gtcttcctcg aagactatct gcagaagcgc ttcactgccg
       61 acagcgtgtt cgtctcgccg gacgccggcg gcgtcgagcg ggcgcgcgcc tactcgaagc
      121 gcctcggggc aaccctcgcc atcatcgaca agcgtcgtga tcgccccaac gtcagcgagg
      181 tgatgcacct catcggcgac gttcgtggtc gcgactgcat catcgtcgac gacatgatcg
      241 atacggcggg caccctctgc aacgcggcgc gggccgcgat ggactacggt gcgcgtcgcg
      301 tcgtggcgtg tgcgacgcac ggagtcctca gcgggcccgc cgtgcagcgc atcgagcagt
      361 cacccctcga agaggtggtg gttaccaatt cgttccctct gtccgaggaa gctcgggctt
      421 gtaagaagat tcgggtgttg tcgatcgggc gtttgctcgg cgaagcgatt cggcgcatcc
      481 acaacagcga ttcggtcagc tctttgttcg tgtgagcgcc gcgtaagagg tcaggagaaa
      541 cgcaccatgg agatcatcaa gatcaacgct agccgccgtg agtcgaacgg caaaggcggc
      601 gcccgccgcg tgcgcaacac gggaagcatc cccgcggttg cctacggcaa agatctcgcc
      661 gcgcaggcgc tcgcgatcgc tcccgcagag ctcagtcgcg tcctcgcgag cgtgcacggg
      721 aagaacaccg tgcttgagct cgacgtcgag ggctcgcaga agatcacggc gctgctccgc
      781 gagtaccagc accaccccgt tacgcgtgag tacctgcacg cggacttcca gcaaattcac
      841 ctcgaccagg aagtcgacgt ggacgtgacg ctcgaagtga cgggcaaggc tgccggcgtc
      901 gtcctcggcg gcacgctgcg ccaggtcttc cgcaagctgc cgatccgctg cttgccggaa
      961 aagatcccgg tgaagatctc gtacgatgtc accgcgctcg gtctcgacgg ccacgtggcg
     1021 gtgcgcgatc tcggcttgcc cgacggcgtg aaggttcggt tgcccccgga gcgcacgctc
     1081 atcgcggtcg tcaaagagaa gcaggctccc gaagaagagg cagcgggcgg ccccgcggct
     1141 gctgcgcctg ccgctgcggc tgctggcggc aaggccgagg ctggcaaggc tggcccgagc
     1201 aagccgcctg agaagaagtg atcctgatcg tgggcctggg gaacccgggc ccgcgttatg
     1261 cggacactcg ccacaacgcg ggcttccagg tcgtcgatcg gctcgtcgag cgagccggcg
     1321 gcgcgagctt ccgcgaaaag ttccacggtc acttcgcgca ggtgaccgtg gacggtgcgc
     1381 agttgtcgct cttgaagccg atgactttca tgaacgagag cgggcgcagc gtgcaggctg
     1441 cggtgcagca cttcaagttg ccgctcgact cgctcttggt cgtacacgac gagctcgatc
     1501 tgccgttcgc cgaggtcagg ttgaagcagg gcggcggcga cgccgggcag cgcggcgttc
     1561 gctccatttc gggagcgctc ggtccgaatt acccgcggct tcgcgtcgga atcggccgtc
     1621 ctccccccga gttcaagggt gacgtcgcag attttgtgct acaagcgtgc tccctcgcgg
     1681 accgggacga gatgcgtgct gcaatcgacc gggccgcgga cgcggtggtg ctcgtggcca
     1741 cgcggggaat atcggccgca atgaacgcga ccaatcagcg caaaacacgc tgactccttg
     1801 ctcccgaacg ttttcggggg ccttcgtccg agaggagatg acatggcaga agcaacagtc
     1861 gcggtgcccc atcgggctcg cgaatacgaa accatctacg tcctgaagcc cgacgtctcg
     1921 cgcgagagcc aagagcgcat cgcgggccgc atcggtgagg tcgtgagccg cgagagcggc
     1981 aagttgaccg ccctcgagaa ctggggtcgt cgccagctcg cctacaccgt cgcgaagtac
     2041 cgtcgcggcg tttacgtgta cgtgaagtac ctgggcggcg gcgcgctcgt ctccgagctc
     2101 gagcgcaacc tgcgcatgct cgacgacgtg ctcaagttcc agacggttca gacggccgtc
     2161 gacgtcgact tcggcaccgt ccaggtcgcc cccgagaacg tccagttcga gtcggtcgag
     2221 ccggccacgc ccgaggagct cgccgagccg atcgagcgct tgctcggtct cgagcagcac
     2281 cccgagcgcg agcgtccttc ggatctggac gacgctgaag aggattacga ggccgcggca
     2341 gaagcagcca aggccgcaga cggcggagag cagtcatgaa agagatgagc cgaggagaac
     2401 gccccagcga gcggccggac gcgcgtggtg gcgacaaaga cgcagccggc aatgcgttcc
     2461 gtcgggttcg tcgtcgtggt tgcgcctact gctccgacga cgccgcgccg atcgactaca
     2521 aggatccgca gagcctcaag tacttcgtga ccgagcgcgg caagatcgtt ccgcgccgca
     2581 tctcgggcac ctgcgcgaag caccagcgca gcatcaccct ggccgtcaag cgcgcgcgca
     2641 acatcgcgct cttgccgttc acgtcgaacg actgaggagg ctcagatggc aacgaacgtc
     2701 aaggttctgt tgaagagcga ggtagagaac ctcggctccg gtggtgaagt ggtgaaggtg
     2761 cgcgcgggct ttgcgcgtaa cttcctcttg ccgcgcggtc tcgcgctgcc cgccactgcg
     2821 ggcaacctga agaacgtcga ggagctcaag cgcgccgcgg cagagcgcgc gaaggcagac
     2881 cgtgagcagg ccgaggagct cgcgaagaag ctcggcgcgc tgtcggtgaa gatcacgcgt
     2941 gcggtcggag aagaaggcaa gatgtacggg tcggtgacgt cgaaggacat cgccgatgcc
     3001 gtgtcgcaga ctggtgtcac gttggaccgc aagcgcatcg tgttgccgga gccgctcaag
     3061 cagctcggta gcttcgatgt cgcggtcaag cttcaccacg acgtgagcac gaccatcaag
     3121 gtcgaagtcg cgaagcagtg agcggttcgg tagtggctga cacgcgcccg actttctgat
     3181 agacgttcag gaactcgggt cggcgctcag cccacccaag aagacgcccc ggaggtcact
     3241 ggcttccggg gcgcttcgca tttgggagcc tgtgtataac gtattgatgg ctttgggaca
     3301 tgccgtgggg tttgcgtgca ttcccaatcc aggtggtgaa ctaggttggt ttcggccggg
     3361 tgcggcccct caccggacat actttgtcct accccaggtt gaattcgttg gattcgatcg
     3421 gatgacaaga cgcatgcgat ggtcgcaggg aacggtttcc aaagaggtgg tgcgatgagc
     3481 ttcggccagc acgggaatcg acaagcagcg caagacttga tgcagctgca accgagtccg
     3541 ggtcgcatcc ccccgaacga cctcgacgcc gaggcagcgg tgctcagcgc cgtgctgctc
     3601 gactcgagct cgttcgacca agtgcaggag accctgtcac cggaccactt ttatgcggac
     3661 gcgaaccgac gaatcttcga agcggtcgtc gacctgcaaa actccggccg tcccgtcgat
     3721 atcgtctcgg ttgccggcta cctgcgcgat cgcaatcgcc tcgaccagat cggtggcacc
     3781 ccgtatctcg cgcagctctc cgatgcgacg ccggccgtcg cgcacgtcgc cgcgcacgct
     3841 cgcgcggtgc gcgagaagtg gcgcttgcga caactcatcg cgacgtgcca gcgctttgcg
     3901 gccgaaggct atgcgacccc gcccgacgtt cagaagttca tcgacgcggc ggagcaagcg
     3961 gtgttcgaca tcgcccgcac gcccgaggga accacggtcg agccagtgcg cgacgcggtc
     4021 gtgcgcgcct tcaagatctt ggaggaggca gagcgccgcg gcggcggcgt caccggtgtc
     4081 ccgactgggt tcacgcgctt cgacaagcag tgctcgggtt tgcacgcggg cgatctctac
     4141 atcgtcgctg cgcgccccgg catgggcaag acgtcgttcg tgctcaacct cgccctgaac
     4201 gtggcggcga cgcgcgcgca aaagagccgc tcgaatggcg aagagtactt cgagcccgaa
     4261 gtggaagagg caggctacgg cgtgctcttc tgctcgctcg aaatgccgaa ggagcagctc
     4321 gcggcgcgct tgctcgcgtc cgaggctcaa gtcgacatgt cgaagatccg cagcggcagc
     4381 atgacgcggg aggactggag tcggctcacc gaggccgctt caaggctcgc gcgtttgccg
     4441 ctctggctcg acgacacgcc cgcgctcacg ctcctcgatt tgcgcgcgaa gatccggcgc
     4501 ctgcaggcgg aggtgtcgcg cggcgagggg gcggctcgcg cgcagaagct cgggctcgtc
     4561 gtgatcgact acttgcagct gatgcagggg cgcaaagacg caggcagccg cgagcaggaa
     4621 atcagcgagc tttcccgc
//
LOCUS       DASTKS010000927         7587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_148401, whole genome shotgun sequence.
ACCESSION   DASTKS010000927 DASTKS010000000
VERSION     DASTKS010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148401"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VFQ35_19425"
     CDS             <1..1295
                     /locus_tag="VFQ35_19425"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET9932888.1"
                     /translation="AVEEPRADVRVTRQGDVALVYAGKTLIVQLFEADARAVGDISLD
                     VLAANVASESQQALESERRRSRVQHNVYSLSLVVFFALIAFYVARKLGEVTARARGWL
                     DENGDRVLAVRVKGIELVTPAVLKSTALVALGLAQWIGQFGIFVAWLVAVLSRFESTR
                     EFTNQLTSFLVSPFSQFMGRLAATLPLVVVASIAALFVFVLVRFLGLFFAGVSRRETS
                     LTWLPPDLAAPASVILRVAIVIAALVFAAPVVTGDTDGALGRAGGIALVALGLASVPL
                     LSSGLLGAVVLFGRRLRVGDHLELGERLGRITAINLLEVRLEASDRTELRVPHLLMLT
                     RAVRGLGARPRLSVEIAAAADAIPSFVVSLLEQAAGRVGRDISVEVVSADLDGVLYRV
                     TAACDSLDARTLLTATLLEGLARAGVSLGRVSGQARRS"
     gene            1292..2548
                     /locus_tag="VFQ35_19430"
     CDS             1292..2548
                     /locus_tag="VFQ35_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kef"
                     /protein_id="HET9932889.1"
                     /translation="MSASELLLGLLVLAYFGGILVGDRTIRGFGLPSGAEYILLGFVV
                     GPRALGLVQSTLIDAFTPIVIVGSAWLALVAGIGYLQVGARRVHLGRALAGVAMSALV
                     GAGIGSAVYFSAALFSSLPHADRLVLAGSAACVSCATTRHAVRWVVERHGAKGKTADA
                     IADYARASVLAPALALALIFGMAPGDGAASLSIPSRVGVTVIVGVVLGLVAALLLGRE
                     FRRDESWGILLGTSLLAMGIAGRLGLSAVSTTFFLGLTVALVSPHRSEVKAMTAPTEK
                     PVMLPVALLAGASVHPQGAPYLAALCVVGIVARLIMELLRGALVNLFVPRTRPAGPLL
                     GLGMLTTGAVSLAAAVALELRLRPDLGASVLGYAVASLLVGELLGPILLRRTLLRVGD
                     IIPGMQQTPPPPSVAPPPVRPSSEVR"
     gene            2545..3906
                     /locus_tag="VFQ35_19435"
     CDS             2545..3906
                     /locus_tag="VFQ35_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HET9932890.1"
                     /translation="MSGHGAAARAKGAITRLVQATALCVIFALLYAATRTAPEFASRL
                     DTVAAVGFLLLAGTLMSELLETIGLPHLSGYILAGILGGPHVLHLVDHHTVEQLVPVN
                     TLALALIAIAGGAEMRIDALREVARSVGIALLLQCAVGLVIMAATFMALARYLPFTSG
                     MSLFALLGVALLWGTLAVSRSPSALLGILAQTRAKGPVATFSLAFVMASDVVVVVLMA
                     CAIMLARPLITPGGSISLDDFNVLGHELLGSVALGTTLGLALAVYLKLVGKNLILVLV
                     ALGFEMSDFMRYVQIDPLLSFLTAGFVVQNLSDQGDHLLEAVEKVGSIVFVVFFANAG
                     AHLDVPLLRSLWPVALALCSARALVTIVTARVASRLVDDPQIIRRWGWSSLISQAGLT
                     LGLSVVIARTFPEFGEGFRSLAIACVAINELVGPILFKFALDRAGETDTHVPARPSLA
                     PPA"
     gene            4415..5788
                     /locus_tag="VFQ35_19440"
     CDS             4415..5788
                     /locus_tag="VFQ35_19440"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9932891.1"
                     /translation="MPKIIAGKYRLVDVLGRGGMGSVWRAEHLTLNSPVAVKLLEPGV
                     AGDPLGLARFMREARAAAKLRSPHVVQILDHGVDEGVPYIVMELLEGEPLANRLAREG
                     RLGLNETVQIVTHIARALSRAHAAGIIHRDLKPDNVFLTRDDDDQPLAKLLDFGIVKS
                     LQGVLETRSEQSTGPGILVGTPYYASPEQAQGTFVLDHRSDIWSLGVIAFECLTGERP
                     FRGESLGDVLLEICVQPMPVPSKRGPVPPGFDEWFAKVCARPRDQRFASAKAAAVALS
                     MLCEAPAPEVREPPLAIPIHVDTPLGTTVREPRVDHARRSTWVGVACALVLTFAVLAY
                     MTRMRIAAAGRSSPASESARVEEVPSRAASPQTSINAALAESPPSVAPGATPEPTGEP
                     GVGSETLDAGVPTPPKGSAEAPPAPPRASVPRPPSRPRVQPSRRPAPSGWTTRERSPT
                     GAPDYGI"
     gene            5877..6773
                     /locus_tag="VFQ35_19445"
     CDS             5877..6773
                     /locus_tag="VFQ35_19445"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9932892.1"
                     /translation="MLDLSVAIAGLLVGFVVGLTGMGGGALMTPVLVLLFGIDPLTAV
                     SSDIVASMVMKPVGGAVHWKRGTVHRRLVLWLMLGSIPSAFLGVVLLRSLGNGAALQG
                     FVKHALGAALLVVVAGLVLRPLLQRNRTKDGGGGLEVRPLPTLLIGIVGGLVVGVTSV
                     GSGSLMIIMLLLLYPRIRLSELVGTDLVQAIPLVASAALGHVLFGDFKLALTGSILLG
                     SIPGVFVGALLSSRAPDYVIRPALVVVLLASGLKLLGVSTSLVSAIGGASALWGIYYA
                     YTAGQRKPAVAQTPALASLRRE"
     gene            6862..>7587
                     /locus_tag="VFQ35_19450"
     CDS             6862..>7587
                     /locus_tag="VFQ35_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932893.1"
                     /translation="MKLELGLCATLILSACGGTSSSVPGKFGSVALDSASTGVPVSAP
                     RAFFYEYTSTGTGVCDTYVDDGICRLWQCNEDAYTSLPSITVLDAGTLGVSGANRELA
                     FDRDEKGSYRVSDFDGTPLWNGGETLTATIGGSSDVPATTLSITAPPPLVVTAPVIPD
                     GGLGINATSDFGLSWQQLTTADSVYVALSTQTDAVTPDGSTVANFAPAVDCKFAGNSG
                     VGIVRSSLLGLLPKPAGLKGYQLD"
BASE COUNT         1020 a         2548 c         2538 g         1481 t
ORIGIN      
        1 tagcggtcga ggaaccgcgc gcggacgtgc gcgtcaccag gcaaggcgac gtcgcgctcg
       61 tgtacgccgg caagacgctc atcgtgcagc tcttcgaagc cgacgcacgc gcggtcggtg
      121 acatttcgct cgacgtgctc gcggcgaacg ttgcgtcgga gtcgcagcag gcgctcgaaa
      181 gtgagcgtcg tcgaagccgg gtccagcaca acgtctattc gctttcgctc gtcgtcttct
      241 tcgcgctcat cgcgttctac gtggcgcgca agctcggtga ggtgacagcg cgcgcgcgcg
      301 gttggctcga cgaaaacggt gaccgggtcc tggccgttcg cgtcaagggc atcgagctcg
      361 tgacgcccgc ggtgctgaag agcaccgcgc tcgtcgcgct cggtctagcc cagtggatcg
      421 gccagttcgg catcttcgtt gcctggctcg tcgcggtgtt gtcgcgcttc gaatcgacgc
      481 gcgaattcac caaccagctg accagctttt tggtcagccc gttctcgcag ttcatggggc
      541 ggctcgccgc gaccctgccg ctcgtggtgg tggcgtcgat cgcggcgctg ttcgtgttcg
      601 tactggtgcg gttcttgggg ctgttcttcg ccggcgtctc ccggcgagag acatcgctca
      661 cctggcttcc ccccgatctg gcggcccccg ccagcgtcat cctccgcgtc gccatcgtca
      721 tcgccgcgct ggtgttcgcg gcgccggtcg tgactggtga caccgacggc gcgctcggtc
      781 gcgcgggcgg gatcgcgctc gtggcgctcg ggctcgcatc ggtaccgctg ctctcgagcg
      841 ggctgctcgg cgcggtcgtg ctgttcggtc ggcggctgcg agttggagat cacctcgagc
      901 tcggtgagcg tctgggccgc atcacggcga tcaacctgct cgaggtgcgg ctcgaggctt
      961 cggatcggac cgagctgcgc gtgccacacc tcttgatgct gacgcgggcg gtgcgggggc
     1021 tcggggcgcg tccacgcctg tccgtggaaa ttgccgcggc cgcagacgcg atcccgtcct
     1081 tcgtggtgtc cctcctcgag caagccgcgg ggcgcgttgg tcgtgacatc agcgtcgagg
     1141 tagtgagcgc tgatctggac ggtgtgctct accgcgtgac tgccgcttgc gactcactcg
     1201 acgcgaggac gttgctcacg gcgactttgc tcgaagggtt agcgcgcgcc ggggtgtcac
     1261 tcgggcgggt gtcgggccag gcgaggcgct cgtgagcgcc agtgagctcc tgctcggcct
     1321 gctcgtgctc gcatatttcg gcggcatcct ggtcggcgac cgcacgattc gcgggttcgg
     1381 gttgccctcg ggggccgagt acatcctgct cgggttcgtg gtgggtccgc gcgcgctcgg
     1441 gctcgtgcag agcacgctca tcgacgcctt cacgccgatc gtgatcgtgg gcagcgcgtg
     1501 gctcgcattg gtggccggca tcggatacct tcaggttgga gcccggcgcg tgcacctcgg
     1561 ccgcgcgctc gcgggcgtcg ccatgagcgc gctcgttggc gcgggcatcg ggagcgccgt
     1621 gtacttctcc gcggcgctgt tctcctcttt gccccacgcc gatcgcctcg tgctcgccgg
     1681 ctcggccgcg tgcgtctcgt gcgcgacgac gcgccacgcg gttcgctggg tggtcgagcg
     1741 ccacggagcg aagggaaaaa ccgcagatgc gatcgcggac tacgcgcgcg ccagcgtgct
     1801 cgctccggcg ctcgcgctcg ctttgatttt cgggatggct ccgggcgacg gcgccgcttc
     1861 gctctccata ccgtcgcgtg tcggcgtaac ggtcatcgtc ggagtggtgc tcggcctcgt
     1921 cgccgcgctc ttgctcggcc gtgaattccg gcgcgacgaa agctggggca tcttgctcgg
     1981 gacgtcgctc ttggccatgg ggatcgcagg gcgcctcggc ctcagcgcgg tttcgacgac
     2041 gttctttctc ggactcacgg tggccctggt ttcgccgcat cggagcgagg tgaaggcgat
     2101 gacggccccg accgagaaac cggtgatgct gccagtcgcc ttgctcgccg gcgcctccgt
     2161 tcatccgcaa ggagcgcctt acctcgcagc actgtgcgtg gtcggcatcg ttgcgcgtct
     2221 catcatggag ctcttgcgcg gagcgctcgt gaacctgttc gtgccacgta cccgaccggc
     2281 ggggccgctc ttgggcctcg gcatgctcac gaccggcgct gtcagtctcg ccgcggcggt
     2341 agcgctcgag cttcggttgc gtccggacct cggtgcgagc gtcctcggtt acgcggtcgc
     2401 gtcgcttctg gtcggtgagc tacttgggcc gatcttgctc cggcgcacgc tgctccgcgt
     2461 cggcgacatc attcccggaa tgcagcagac cccaccacca ccgagcgtcg cgcccccgcc
     2521 cgtgcgccct tcttcggagg ttcgatgagc gggcacggcg ccgccgcgcg cgcaaaagga
     2581 gcgatcacgc gcttggtgca ggcgaccgcg ctgtgcgtaa ttttcgcgct gctctacgca
     2641 gcgacgcgca cggcgccgga gtttgcgtcg cggctcgaca ccgttgcggc ggtcggcttc
     2701 ttgctgctcg cgggtaccct gatgagcgag ctgctcgaga ccatcgggct gccgcacctt
     2761 tcgggctaca tcctcgctgg catcctcggt ggcccccacg tcctccacct cgtcgaccat
     2821 cacactgtcg agcagctcgt gcccgtgaac accttggcgc tcgccctcat cgcgatcgcg
     2881 ggcggcgccg agatgcgcat cgacgcattg cgtgaagtcg ctcgcagcgt gggcatcgcg
     2941 ctgctactgc aatgcgcggt tggtctcgtc atcatggccg cgaccttcat ggcgctggcg
     3001 cgctacctgc cgttcacgag cgggatgagc ctcttcgcgc tgcttggcgt cgcgctcttg
     3061 tggggcacgc tcgcggtgag ccgtagtccg tcggcgctgc tcggtatcct cgcgcagact
     3121 cgagccaagg ggcccgtcgc gacgttctcg ctcgcgttcg tgatggcgtc cgacgtggtc
     3181 gtcgtcgtgc tgatggcttg tgcgatcatg ctggcgcgcc cgctgatcac gccgggcggc
     3241 agcatctcgc tcgacgactt caacgtgctc gggcacgagc tcttggggag cgtcgccctc
     3301 ggtacgacgc tcgggcttgc gttggcggtg tacctcaagc tcgttggcaa gaacctcatc
     3361 ctggtgctgg tcgctctcgg gttcgaaatg agcgacttca tgcgctatgt ccagatcgac
     3421 cccctgctct cgttcctcac ggccggcttc gtcgtacaaa acctgtccga ccaaggtgat
     3481 cacttgctcg aagcagtcga gaaggtgggt agcatcgtgt tcgtcgtctt tttcgcgaac
     3541 gcgggagcgc acctcgacgt acctctgttg aggagccttt ggcccgtagc gttggcgctg
     3601 tgctcggcgc gtgccctcgt cacgatcgtc actgcgcgag tcgcgagccg cctcgtcgac
     3661 gacccgcaaa tcattcgtcg ctggggttgg tcctcgctca tctcgcaagc agggctcacc
     3721 cttgggctca gcgtcgtcat cgctcggacc ttccccgaat tcggtgaagg atttcgctcc
     3781 ctcgcgatcg cgtgcgtcgc aatcaacgag ctcgttggcc ccatcctctt caaattcgcg
     3841 ctcgatcgag cgggagagac ggacacgcac gttcctgctc gcccgagcct cgccccgccc
     3901 gcctgaagcg agttcgagtt acgcatcggg tgtaagaccc cgaagcgttc ggggaccgaa
     3961 gcggggcatt cccgcgcgcg aaagggctgg gtgaggcacg tcggcgcgct tctggttgcg
     4021 cagaagactt cgctggcgcg cttctctgcg acccgagcga ggccccagga gccgtgaatt
     4081 catcagcaaa tcgaatacga gaagactgcg taggcgattt cgcgcgcgcc gcccgggagt
     4141 tcggcacgcg gtttggatcg tgcaggtcct ggctgggatc cggtctcgca caagttcgcg
     4201 aaagcacacc atgacgcaac ccaagcaaag ccccacccga gcttactcgc acgtgagtgc
     4261 gttggggcag aattgcgggg tgcggcgcgc cgccacgctt tcgggtgacg agcacgcaga
     4321 atcgcagtcg ctcccgtgcg ctccgccgtc gcccgtcgat ggatcggcat cacagttcga
     4381 gccggccgca gcggaatcca aactgcctga atcggtgccc aagatcatcg ccgggaagta
     4441 ccgtctcgtc gacgttctcg ggcggggagg gatgggctcc gtttggcgcg ccgagcacct
     4501 cacgctcaac tcgcccgtcg cggtgaagct gctcgagcct ggcgtcgcgg gggatccgct
     4561 gggccttgct cgtttcatgc gcgaagcgag ggccgcggcc aagctacgca gcccgcacgt
     4621 ggtgcagatc ctcgatcacg gcgtggacga gggcgtccct tacatcgtga tggaactctt
     4681 ggagggcgag ccgctcgcga accgcttggc gcgcgagggg cggctcggct tgaacgagac
     4741 cgttcaaatc gtcactcaca tcgcacgcgc gctttctcgc gctcacgccg ctggcatcat
     4801 tcaccgcgac ctgaagcccg acaacgtctt tttgactcgc gacgacgatg accagccact
     4861 cgccaagctg ctcgatttcg gcatcgtgaa gagcttgcaa ggtgtgctcg agacacgcag
     4921 cgagcaaagc acggggcccg ggattctcgt gggcactccc tactatgcga gtccggagca
     4981 agcgcaggga acgttcgtgc tcgaccaccg gagcgatatt tggtcgctcg gcgtgatcgc
     5041 gttcgagtgc ctcacgggcg agcgaccctt tcgcggtgaa tcgctcggcg acgtcctgct
     5101 cgagatttgc gtccagccga tgcccgttcc gtcgaagcgc ggtcctgtcc cacccggctt
     5161 cgacgagtgg ttcgcaaagg tctgtgcccg cccgcgcgac cagcgcttcg cgtcggccaa
     5221 ggccgcggcg gtcgcgctct cgatgctctg cgaggctccc gcgcccgagg ttcgcgaacc
     5281 cccgctcgcc atccccattc atgtcgatac gccgctcggg acgacggtgc gcgagccgcg
     5341 cgtagaccac gcgcgccgga gtacctgggt tggggtagcc tgtgcgttgg ttctgacctt
     5401 cgccgtcctc gcgtacatga cgcgcatgcg catcgcagcg gctgggcgca gctcgcccgc
     5461 gtcggagtcc gcgcgcgtcg aggaagtgcc gagccgcgcc gcgagccccc agacgtcaat
     5521 caacgccgcg ctggcggagt ctccgcccag cgtcgcgccc ggtgcgacac ccgaacccac
     5581 gggcgagccc ggcgtcggct ccgaaacgct cgatgctggg gtgcccacgc ccccgaaggg
     5641 ctcggcggaa gctcctccgg cgcctcctcg cgcgagcgtc ccccggcctc cctcccgtcc
     5701 gcgcgtccag ccttcgcgac gcccggctcc ttccggatgg accactcggg aaagatcgcc
     5761 gacgggcgcc cctgactacg gcatatagtc ttaactgttg gatgtttaag gacattattc
     5821 tttctctaat tctctactag acttatcggt cattcgccgg cataccttgg gtcagcatgt
     5881 tggatctgag cgtcgcaatc gccgggcttc tcgtgggctt cgtcgttggg ctcaccggca
     5941 tgggtggggg cgcgctcatg actcccgtcc tcgtgctgct cttcggcatc gaccctctga
     6001 ccgcggtttc gagcgacatc gtggcctcga tggtgatgaa gccggtcggc ggcgccgtgc
     6061 actggaagcg cgggacggtg catcgccgcc tcgtgctctg gctcatgctc ggctccattc
     6121 caagcgcgtt cctcggggtg gtgttgctgc gctcgctcgg caacggcgct gcgctgcagg
     6181 ggttcgtcaa gcacgcgctc ggcgcggcgc tgttggtcgt cgtggccggc ctcgtgttgc
     6241 ggcctctcct ccagcgtaac cgcaccaaag atggtggagg agggctcgag gttcggcctt
     6301 tacccacgct tttgattgga atcgtgggcg ggctcgtcgt gggcgtgact tcggtcggct
     6361 cggggtcgct catgatcatc atgttgctgc tcctctaccc gcgcattcgg ctgtccgagc
     6421 tcgtcggaac ggatctcgtg caagcgatcc cgctggtcgc gtcggcggcg ctcgggcacg
     6481 tgctgttcgg tgacttcaag ctcgcgctga ccggttcgat tctccttggg agcatcccgg
     6541 gcgtcttcgt cggagcactc ctctcgtcgc gcgctccgga ctatgtcatt cgcccggcgt
     6601 tggtcgtggt gttgctggct tcgggcttga agctgctcgg cgtttctacg agcttggtga
     6661 gtgcgattgg cggcgctagc gcgctctggg gcatctacta cgcgtacacg gctgggcagc
     6721 gaaagccggc ggtcgcccag actccggcgc tcgcctcact gcgccgagag tagtcggcac
     6781 cgcaacgaat cgaaacatcg cgcggaggac ccgcgggttt gggatctgct aaggttccgc
     6841 gcccgtcggg cggagcctgc gatgaaactc gagcttggat tgtgtgcgac cctcattctg
     6901 tcggcgtgcg gcggtaccag cagcagcgtg ccgggtaagt tcggcagcgt cgcgctcgac
     6961 tcggcttcga cgggcgtgcc ggtgagcgcg ccgcgcgctt tcttttacga gtacacgagc
     7021 actgggaccg gcgtgtgtga cacgtacgtc gacgacggca tttgccgcct gtggcagtgc
     7081 aacgaggacg cctacacgag cctgccgagc attaccgtgc tcgacgcggg cacgctcggc
     7141 gtaagcggcg cgaatcggga gctcgccttc gaccgagacg agaagggttc ttatcgcgtc
     7201 agcgacttcg acgggacgcc cctatggaat ggcggcgaga cgctcacggc gaccatcggc
     7261 ggctcgagcg acgtgccggc gacgaccctg tcgatcacgg cgcctccgcc gctcgttgtc
     7321 accgctcccg tcatccccga tggcggtctc ggcatcaacg cgaccagcga cttcggcctc
     7381 agctggcaac agctgaccac cgcggactca gtttacgtcg ctctgtccac ccagaccgat
     7441 gccgtaacgc ccgacgggag taccgttgcg aactttgccc ccgccgtcga ctgcaagttc
     7501 gcggggaact ccggcgtcgg catcgtgcgc tcgtcgctgc tcggcttgct cccgaagccg
     7561 gccgggctga agggctatca gctcgac
//
LOCUS       DASTKS010000928         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1041098, whole genome shotgun sequence.
ACCESSION   DASTKS010000928 DASTKS010000000
VERSION     DASTKS010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041098"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..642
                     /locus_tag="VFQ35_19455"
     CDS             313..642
                     /locus_tag="VFQ35_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932894.1"
                     /translation="MQLRGGLRAPTNFPVVAGTGRQRRECRAVELSPSGIVLASTDAG
                     ERPMPLVVRIEMRLPERLRPLIAWARPIWARGEYQALKFVGASDADRLSLAEHLDLQQ
                     LRGFTPK"
     gene            663..1508
                     /locus_tag="VFQ35_19460"
     CDS             663..1508
                     /locus_tag="VFQ35_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932895.1"
                     /translation="MNPLSKEFWSSVGAIVLAAPRVLVPLGALAALSSGMTGCVSRLS
                     YEEAASAAEVEREGHRRAELDLAALKARVAELEGQLKERSAGLSEREEKLAEEKYERN
                     VASKERDETASLVAQLRGELARANEHLEAYARNNARLEHELSVSRDGGASSPLVQELK
                     SVLTSAHLEQSVRLSENATGVTASVPADIVFKRGEAELGPALGSLSAALARFAESHPE
                     LRVTLREGAPDPALPDSIGKQRRDSLRALLSHPKLGSRVTFEPASGSAAPASYELTLA
                     FAGQR"
     gene            complement(1577..1819)
                     /locus_tag="VFQ35_19465"
     CDS             complement(1577..1819)
                     /locus_tag="VFQ35_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932896.1"
                     /translation="MHREEREYSIVLNLVAEFGDDYEGDADGFAWHETFERTLKPRLV
                     AAVFEALKSDPRLQIVPAPRGRDPERALELDVLRRY"
     gene            complement(1872..>2328)
                     /locus_tag="VFQ35_19470"
     CDS             complement(1872..>2328)
                     /locus_tag="VFQ35_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932897.1"
                     /translation="VSFFLKVDSDGKVSESYLEHSTLGDRDTEKCMLSAVRGRKWPKP
                     VGGMHGLARKSFDFDPPNDVRPPVEWDSDQVKSALKKMGDKLDECRDGVSGTFEATAY
                     VSTEGRVLAASVTPPNADGERAVDCLVSAIREGSFPSPGSWPAKVTFQL"
BASE COUNT          386 a          795 c          783 g          364 t
ORIGIN      
        1 cgcgatgctc gtcgacgtgg atgcgcgaga gcgaaacctt ggcttcggcc gcggagcctt
       61 cgccgcgcgc cgagcgcgcg ccaacgagcg cgacgccgac cacgagcgcg cgcgagaggc
      121 tcttgaacca cggtcctcgc gccactcgcc tcccataagc gctccaggga aattcaccaa
      181 atttctggct ccatcacgaa ctgcttcgtt aagtctcgga gcgcgcgtcg ggcccgcttc
      241 gccaagtggg acgagggaaa gttggccgcg tggatcggcg gcgtcttacg atttctcgcg
      301 aggagcgcac ccatgcaact acgaggcggt ctgcgagccc cgacgaactt tccggtcgtc
      361 gcgggaaccg gtcgacagcg gcgcgaatgt cgcgccgtcg agctcagccc gagcggcatc
      421 gtgctcgcta gcaccgatgc cggcgagcgt ccgatgcccc tcgtggtccg catcgagatg
      481 cggctgcccg agcgcctgcg gccgctcatc gcctgggccc gccccatctg ggcgcgcggc
      541 gagtaccagg cgttgaagtt cgtgggcgcg tccgacgccg atcgtctttc gttggcggag
      601 cacctggatt tgcagcagct gcgcggcttc acccccaagt aaccccatcc atgacatcga
      661 aaatgaatcc cttatccaaa gagttctggt cgagcgtagg cgcaatcgtg ctcgcggcgc
      721 cgcgcgtcct cgtcccgctg ggcgcgctcg ccgcgctgag cagcggcatg accggttgcg
      781 tatcacgcct ttcgtacgaa gaggccgcgt ctgccgccga agtcgagcgc gaggggcacc
      841 gccgcgctga gctcgacctc gccgcgctga aagcgcgggt cgccgagctc gaaggccagc
      901 tgaaagagcg cagcgcgggc ctgagcgagc gcgaagaaaa gcttgccgag gaaaagtacg
      961 agcgcaatgt tgccagcaag gagcgcgacg aaaccgcgtc gctcgtggcg cagctgcgcg
     1021 gcgagctcgc gcgcgcgaac gagcacctcg aggcctacgc gcgcaacaac gcgcggctcg
     1081 aacacgagct cagcgtgtcg cgcgatgggg gcgcgtcatc gccgctcgtc caggagctga
     1141 agagcgtact caccagcgct cacctcgagc aaagcgtgcg gctctccgag aacgcgactg
     1201 gcgtgaccgc gagcgtgccc gccgacatcg tcttcaaacg cggcgaggcc gagctcgggc
     1261 cggccctggg ctcgctctcc gccgcgttgg ctcgatttgc cgaatcccac ccagagctgc
     1321 gcgtgacgct gcgtgagggc gctcccgatc cggcgttgcc cgactcgatc ggcaagcagc
     1381 gccgcgacag cctacgcgcg ctgctgtcgc acccgaagct cggtagccga gtgaccttcg
     1441 aaccggcgag cggctccgcg gcgccggcga gctatgaact gacgctcgcc ttcgcagggc
     1501 aacgctagcc tcggtcgtgc ggcgccgcgc tccgacgacc aagcaacgac ggaggctggg
     1561 tccgaaggac acctcgctag tagcggcgca gcacgtcgag ttcgagcgcg cgctcgggat
     1621 cacgcccgcg cggcgcaggc acgatttgga gcctggggtc gctcttcagc gcctcgaaca
     1681 ccgcggcgac gagccgtggc ttcagcgtgc gctcgaacgt ctcgtgccaa gcgaacccgt
     1741 cggcgtcgcc ttcgtagtcg tcgccgaact ccgccacgag gttcagtaca atcgagtatt
     1801 cgcgctcttc gcggtgcatc gagcggctat gccacggcgg gcgagagcgc gcaccgcgct
     1861 gcggggaccc ttcagagctg gaacgtaacc ttcgccggcc agctgccggg tgaaggaaag
     1921 gagccttcac ggatcgcgct cacgaggcag tcgaccgcgc gctctccgtc ggcgttgggt
     1981 ggcgtgacgc tcgcggcgag cacgcggccc tcggtcgaga cgtaggcggt cgcttcgaac
     2041 gtacccgata cgccgtcgcg acactcgtcg agcttgtcgc ccatcttttt gagcgcgctc
     2101 ttcacctggt cgctgtccca ttcgacggga gggcgcacgt cattgggcgg gtcgaagtcg
     2161 aacgacttgc gtgcgaggcc gtgcataccg ccgactggtt tcggccactt gcgtccacga
     2221 accgcagaga gcatgcactt ctcggtgtcg cgatcgccga gggtcgagtg ctcgaggtac
     2281 gactcggaga ccttgccgtc cgaatcgact ttgaggaaga agctgacc
//
LOCUS       DASTKS010000929         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1041665, whole genome shotgun sequence.
ACCESSION   DASTKS010000929 DASTKS010000000
VERSION     DASTKS010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041665"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFQ35_19475"
     CDS             <1..653
                     /locus_tag="VFQ35_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HET9932898.1"
                     /translation="LSQSEASAGGLKEIVALVSGQRVYSSLKYEGGVHRVQRVPATEA
                     QGRIHTSTATVAVLPEADEIDIEIDDKDLRFDIAASGGPGGQGVNTTNSAVQITHLPT
                     GMIVKCQDERSQLKNKAKALKILRSRLLDIEREKQAEAQRDERRGMVKAGDRSEKIRT
                     YNFPQNRLTDHRIGLTLYELDRIIEGDLDQLIAALGAWHQAQLLDTDPDGSGASGP"
     gene            650..1075
                     /locus_tag="VFQ35_19480"
     CDS             650..1075
                     /locus_tag="VFQ35_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932899.1"
                     /translation="MKVSTEVDDSALFAALVLVPATFSRNRFFGLFEDPERKRLRRRA
                     ARVRGIVRQLVSPERGRAEIVGERVLEDGRVLLRYTVDEMGYSRTASLSKLEASVLRY
                     ALARAGLGTLPDEDKKVVEQSLSRLGKGLGLALDGVASE"
     gene            complement(1079..1969)
                     /locus_tag="VFQ35_19485"
     CDS             complement(1079..1969)
                     /locus_tag="VFQ35_19485"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9932900.1"
                     /translation="MSDDSTPRFTSGAGKAEPHPLNLEVRRFWLEHGDAKISLEPRTY
                     VLGRSSQCELMVDDARASRRHARLVVRALTVSIEDLQSSNGVFVNGSRIVKVQTLRDG
                     DQFQVASASFVLKSEGLPFRSGSETPATSPNLIRHSEHPSLSPEDAEAPTVEAHSLDL
                     LGTLAEKSLALGRADEAEHMLSNALDELLRRARTRRIRDVPSRIFAKAAHYAVLLAEA
                     TQRGAWVDYAIALFDAIQRPLPSAVVDQLYITVRKTPDVNSAALRNYVERLRACADAS
                     PADRFVIQRLAGLERQMSPK"
     gene            2361..>3681
                     /locus_tag="VFQ35_19490"
     CDS             2361..>3681
                     /locus_tag="VFQ35_19490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET9932901.1"
                     /translation="MAETERLRSPAVTQRSKSGPLAERGFLARRLTAAMLTPLALLLL
                     LGLVLGRQILEMADDAQWVDHSDRVVSAANRTLLEVLNQETALRGFLLVEDPSMLEPF
                     RRANPLGGFDELKALTADNPAQERRFAEARRRYEDWSVAATMKDPSAELRGMREPRVL
                     LQRKAKMDAFRDAMRSALDVEIGLRKTRAETSQRSTEMTKQLFVLLVGASAAVLAFLS
                     RRQLSSIASTFADALAAEKKARDAVESEAWVRSGHAKLTATLQGEKTIVELGEACLAV
                     LTKLTSADVGAFFTRDHGHWRRRAGYALDSRAAGQELFPLGEGLVGRAAQSGELLDLR
                     DVPAGYLEVRSGTGASRPLELFVVPAQSDGETVAVLELAYLREATPEARALLSRIGET
                     LATAVRSSEYKQHLRELLEETQRQAEELQTQQEELRVSNEELEQQSQA"
BASE COUNT          661 a         1218 c         1200 g          602 t
ORIGIN      
        1 tgctgtcgca gagcgaagcc tccgcgggtg gcctgaaaga aatcgtggcg ctggtcagcg
       61 gccagcgcgt ctactcgagc ctgaagtacg agggcggcgt acaccgcgtg cagcgcgtac
      121 cggcgacgga ggcacagggc cgcattcaca cctcgacggc taccgtcgct gttttgccgg
      181 aagccgacga gatcgacatc gaaatcgacg acaaggatct gcgtttcgac atcgcggcct
      241 ccggcgggcc tggcgggcaa ggcgtgaaca cgaccaacag cgccgtgcag atcacccatt
      301 tgcctaccgg catgatcgta aagtgccaag acgaacgcag ccagttgaag aacaaggcga
      361 aggcgctgaa gatcttgcgc agccgcttgc tcgacatcga gcgcgagaag caagccgagg
      421 cgcagcgcga cgaacgtcgc ggcatggtta aggccggcga tcgctccgag aagatccgca
      481 cttacaactt ccctcaaaac cgcctcaccg atcatcgcat cggactcacg ctctacgagc
      541 tcgatcgcat catcgaaggt gatctcgacc agctcatcgc agcgctcggt gcgtggcacc
      601 aggcacagct cttggacacg gatcccgacg ggagtggggc gagcggtccg tgaaagtgag
      661 caccgaggtc gacgactcgg cgctcttcgc ggcgctggtg ctggttccgg cgaccttctc
      721 gcgcaaccgc tttttcggat tgttcgaaga tcccgagcgc aagcgcttgc ggcgccgagc
      781 cgcgcgcgtg cgtggcatcg tgcggcagct cgtgagcccg gagcgtgggc gcgccgaaat
      841 agtcggcgaa cgcgtgctcg aagacggacg cgtgctccta cgctatacgg tggacgagat
      901 ggggtactcg cgcaccgctt ccctctcgaa gctcgaggcc tcggtgcttc ggtatgcgct
      961 agcccgcgcc ggcttgggca cgctccccga cgaagacaag aaggtcgtcg agcagagcct
     1021 ttcgcggctc ggcaaggggc tcgggctcgc gctcgacggc gtcgcgagcg agtgagcgct
     1081 acttcggcga catttgacgc tcgagcccag ccaggcgctg aatcacgaag cgatcagcgg
     1141 ggctcgcgtc cgcgcatgcg cgcaggcgtt cgacgtagtt ccggagcgcg gcgctgttca
     1201 cgtccggggt cttgcgaacg gtgatgtaga gctggtcgac gaccgccgaa ggcagaggcc
     1261 tctggatcgc gtcgaacagc gcgatcgcat agtcgaccca cgcgccgcgc tgcgtggctt
     1321 cggcgagcag gacggcgtag tgggccgctt tcgcgaagat gcgcgacggc acgtcccgaa
     1381 ttcgccgcgt gcgtgcgcga cgcaggagct cgtcgagcgc gttactcagc atgtgctcgg
     1441 cttcgtccgc gcgccccagc gcgaggctct tttccgccag cgtaccgagc aagtcgagcg
     1501 agtgagcttc gacggtaggc gcctcggcgt cttccggcga caagctcggg tgctcgctgt
     1561 gacggatcag gttcggtgac gtcgccggtg tctcactgcc gctgcggaac ggcaacccct
     1621 cggacttcag cacgaacgac gcgctcgcca cctgaaactg atccccgtcg cgcagcgttt
     1681 gcaccttcac gatgcgcgag ccgttgacga acacgccatt cgagctctgc aggtcttcga
     1741 tgctcaccgt caacgcccgc acgacgagcc gcgcgtggcg acgcgacgcg cgcgcgtcgt
     1801 cgaccatcaa ctcgcactgg ctgctgcgac ccagaacgta ggtcctgggc tcgagagaga
     1861 ttttcgcgtc cccgtgctcc aaccagaacc gacgcacctc gagattcaat gggtgcggct
     1921 cggctttgcc tgcgccagac gtgaatcgcg gagttgaatc gtcgctcatc gaccgatgga
     1981 ccggaaccta tcgtctccta cgttcaatgt tgccgattgc gaagcgcacg aagcgcgggc
     2041 ttctgctatt cgcttcgatt tggaggagtg tcgccccgcg agccgcacta ccacccaggt
     2101 attcacacac cgagttttgg tgcccgctga atcagaacga gacgccgcgc gttcggcgat
     2161 ggcgccctgc gccaggcgcc gtcgcgaaac tttatccctc gcacggcacg atccccctcg
     2221 acaaacgtgc tgagttcgcg ggtgccaaag cgccgcagcg tggcgcgttt gtcgcaactc
     2281 cgatctatgt cgacctccga cgccgacgcg attgtggact ctctgccaac gggttacgtc
     2341 gtctagcggc tcgaacgtcc atggcagaaa ccgaacgact ccgaagcccc gccgtcacac
     2401 agcgttcgaa gagcggtccg ctcgccgaac gaggttttct cgcgcggcgg ctgacggccg
     2461 cgatgctgac gccgctcgca ctcctgttgc tgctcggcct cgtcctcggg cgtcaaatct
     2521 tggagatggc ggacgacgcg caatgggtcg accacagcga tcgggtcgtc agcgcggcca
     2581 accgtacgtt gctcgaggtg ctgaaccaag agacggcgct gcgtggcttc ttgctagtgg
     2641 aagacccatc gatgctcgaa cccttcaggc gcgccaatcc gctcggcggc ttcgacgagc
     2701 tgaaagcgct caccgcggac aaccctgcgc aggagcgacg cttcgccgag gctcggcgcc
     2761 gctacgaaga ctggtcggtc gccgccacca tgaaagatcc cagcgccgag ctgcgcggga
     2821 tgcgtgagcc gcgcgttttg ctccagcgca aagcgaagat ggacgctttt cgcgacgcga
     2881 tgcgaagcgc gctcgacgtc gagatcgggc tccgcaagac gcgcgcagag acctcgcaaa
     2941 gatcgaccga gatgaccaag cagctcttcg tgctgctggt cggcgcctct gcggcggtgc
     3001 tcgcgttcct gtcgcgacgg cagctgagct cgatcgcctc gactttcgcc gacgcgctcg
     3061 cggccgaaaa gaaggcccgc gacgcggtcg agagcgaagc gtgggtgagg agcggtcacg
     3121 cgaaactcac ggccacattg cagggcgaaa agacgatcgt cgagctcggc gaggcgtgcc
     3181 tcgcggtgct caccaagctg acctcggcgg acgtgggcgc gttcttcacg cgcgatcacg
     3241 ggcactggcg ccgacgcgct ggttacgcgc tcgactctcg cgccgcgggg caagagctgt
     3301 tccccctcgg cgaaggcttg gtcggccgcg ccgcgcaaag cggcgagctc ttggacctgc
     3361 gcgacgttcc cgccggttac ctcgaagtgc gctcggggac aggcgcatcc cggcccctcg
     3421 agctcttcgt cgtgccggcc cagagcgacg gcgaaacggt cgcagtactg gagctcgcct
     3481 acttgcggga agcgaccccc gaggcccgcg ctctcctctc tcgcatcggc gagactttgg
     3541 cgacggccgt gcgctcgtcc gagtacaaac aacacttgcg cgagctactc gaggagactc
     3601 agcgccaagc cgaggaactg cagacccagc aagaggagct gcgcgtctcc aacgaagagc
     3661 tcgaacagca gagccaagcc c
//
LOCUS       DASTKS010000930         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1712207, whole genome shotgun sequence.
ACCESSION   DASTKS010000930 DASTKS010000000
VERSION     DASTKS010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712207"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFQ35_19495"
     CDS             complement(<1..1273)
                     /locus_tag="VFQ35_19495"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HET9932902.1"
                     /translation="MARSVYARLLVIVALFEITGCGAPDQVDAFPAESRSTALENEFP
                     GSRGFEVTHPASGTLEAYASHASATAGDTVDVFVNSTESDDAFFEVYRIGYYQGRGGR
                     LIRRGASQHVEPQASCPVDAATGLIECAWQKTFSIALDESYTTGYYVIKVIASRGVET
                     RLPLVIREPAPHAPILVQASTNTWQAYNYWGGTSLYVNFLSKTEQYAGQRAYRVSYDR
                     PYALDDGAGALFDQEAWMARWLEQKGYEVAYVTNIDVDREPSLLLGRKMVFSVGHDEY
                     NSVTTRDAFEAARDAGISLGYYSANAGFWRVRQDPSSSGVERRIITCYKDSSLDPVRD
                     SLETTAQFRQAPFARPESLLEGVMYQAGNAPHSDLPMLVSAPESWVFEGTGVVAMERL
                     ANVLGPEWDQVADEEPANAEILFDSPAIRKLG"
     gene            complement(2267..2398)
                     /locus_tag="VFQ35_19500"
     CDS             complement(2267..2398)
                     /locus_tag="VFQ35_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932903.1"
                     /translation="MLPRRQPSGHHMLVWLLALVVIAVVLVLASALLRALLKKHGIM"
     gene            2622..3131
                     /locus_tag="VFQ35_19505"
     CDS             2622..3131
                     /locus_tag="VFQ35_19505"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HET9932904.1"
                     /translation="MFFQVVSQFKRQLQQIESWFDAAQNFAKERAFDPNVFASQRLAP
                     DQFALARQVQIACDTAKFAVARLSGKDAPNHPDTEQTLDELRGRIRSVVGYLDGFSAK
                     DFEGAATRTISQPRWEGKTMTGADYFVEHVVPNFHFHFTHVYAILRHNGVPLGKKDYL
                     GAITMKSPG"
     gene            complement(3211..4866)
                     /locus_tag="VFQ35_19510"
     CDS             complement(3211..4866)
                     /locus_tag="VFQ35_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9932905.1"
                     /translation="MESLEREFDFVIVGSGFGGSVSAMRLAQKGYRVAVLEAGKRYRA
                     TDFPKSNWNVAKYLWAPRLRCFGIQNITLLKGVMVLHGSGVGGGSLVYANTLMQPHEH
                     VFDDPAWRGAVDWKRELAPHYETARRMLGVTTNPALFEGEEALRRVSERMGAESTFHP
                     TEVGVFFGEPNRRVPDPYFSGAGPERVGCNFCGGCMVGCRFGAKNTLDQNYLFFAEKW
                     GAEVFPETKVTRIRPVEGGYELETEVSTSAFRKRGRAFKARRVIVAAGVLGTVELLLR
                     NRDVYRTLPNISERLGDFVRTNGESLLGATSFEAHRELSRGIAIGAAFHPNSLTKIES
                     VRYPSGSGVMRLLGVPITPNGSALTRPLKMLGRILRRLPRVVRLWRVADWARSTVILL
                     VMQSVDEHLRLRLGRSILRRALFGLEGAPTERPVPSYLPVAQEAAEILGEEIHGEPQN
                     VISEVMLRTPATAHILGGCCIGDAPENGVIDARHQVFGYEGLYVCDGSVVPGNLAVNP
                     SLTISALAERFAHQFEPAPGFSTEELARREIRFGASARSLGRG"
     gene            complement(4889..>5364)
                     /locus_tag="VFQ35_19515"
     CDS             complement(4889..>5364)
                     /locus_tag="VFQ35_19515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932906.1"
                     /translation="ISVKSEQGKGSTFCVDLPFIIGAPEEKAAPRRGSARGDELILVA
                     EDEPLVRAQAVRILKRAGYNVLEAEDGLKAVDVFESNPDVALVLLDVIMPGLDGWQAF
                     SRIEAIRKGVKVLFITGYAASALPPDFGRLGKRLLSKPYMPQALLDLVRELLDHT"
BASE COUNT         1023 a         1754 c         1651 g          936 t
ORIGIN      
        1 caccgagctt gcgaatcgcc ggtgagtcga agagaatttc ggcgttggca ggctcctcgt
       61 ctgcgacttg atcccactcc ggcccgagca cgttcgcgag gcgctccatc gcgaccacgc
      121 ccgtgccctc gaagacccaa gactcgggcg ccgacacgag catggggaga tcggagtgag
      181 gagcattgcc ggcctggtac atcacccctt cgagcagtga ctctgggcga gcgaaaggag
      241 cttggcgaaa ttgcgctgtc gtctcgagcg aatcgcgcac tggatcgagc gacgaatcct
      301 tgtagcaagt gatgatccgc ctttctacgc cgctgctcga ggggtcttgg cgaacgcgcc
      361 aaaagcccgc gttggcgctg tagtagccga gcgagatgcc ggcgtcgcgc gcggcctcga
      421 aagcgtcgcg cgtcgtcacc gagttgtact cgtcgtgccc gaccgaaaag accatctttc
      481 gaccgagcag caacgaaggc tcgcgatcga cgtcgatgtt cgtgacgtac gcaacctcgt
      541 accccttctg ttcgagccag cgcgccatcc acgcttcttg atcgaacaag gcccctgctc
      601 catcgtcgag ggcgtaagga cgatcgtagc tgacgcgata cgcgcgctga cctgcatact
      661 gctcggtctt ggagaggaaa ttgacgtaaa gggacgtgcc tccccagtag ttgtacgcct
      721 gccaggtgtt cgtgctcgct tgcaccagga ttggcgcgtg cggcgccggc tcgcgaatga
      781 ccagcggcaa acgcgtctcg acgccacgcg acgcgatcac tttgatgacg tagtagccag
      841 tcgtgtagct ctcgtcgagt gcgatgctga aggtcttctg ccacgcgcat tcgatgagcc
      901 ccgtcgccgc gtcgaccggg caactcgcct ggggctcgac gtgttgactc gcaccgcgac
      961 gaatcaagcg gccgccgcgg ccctgatagt atccgatgcg atagacttcg aaaaaggcgt
     1021 cgtccgactc ggtgctattc acgaatacgt cgaccgtgtc gcccgccgta gccgaagcgt
     1081 gcgaagcata cgcttcgaga gtcccgctgg ccgggtgcgt cacctcgaac ccgcgactac
     1141 cagggaactc gttctccaac gcggtcgagc gagattcggc tggaaacgca tcgacctggt
     1201 cgggagcccc acatcccgtg atttcgaaca gagccacgat aaccagaagc cgagcgtaaa
     1261 cggagcgcgc cattccgttc ctttcaccta tccgcgcgga gcacctctcc ccgggcggtg
     1321 ctgcactcta gccagcgatc gcagtcgcgt cagccgaaaa tagtcatctc ggtcggctca
     1381 ttctgattca cgaggatgaa gtgacctttc gagcgaacat cagttcgtta aaccaaataa
     1441 tgatgacgat ttgtctcgcc gattcggctc tacaccagcg accgcgtcgg cgcaccgcaa
     1501 gattcgcgcg actttgcagg agtgaccgca aagcccgcga tccgtcgctc gatggtcact
     1561 tttctcgtcg agtcgggcgc accctcgagc gatgctgcag tcgcagaaaa ttcgaaccag
     1621 ccatcggagc ggtgaaagaa tgtctcgagg gtcgatgacg cgagccgcgg aattttttcg
     1681 gggccgctga gcatgaacgg cccgagccct cggcggattt ttccggtacg agttgaggcg
     1741 cgccgttctg ccacaatgtg ccaaagcgcg ccgcaagtcg ccgccggcgc tacgcggcac
     1801 ccgagcgctg ggcacgaggg gtgcaagttc acgtgctcgc tggcgccgat cgccagcttc
     1861 actccatgct tcgacgtcgc agttctgcgc tcgacgcgcg ctcattcagg tcgacgctca
     1921 tcgccgtgtg cctcgttgcc tgcggcggca gcgcgcatcg cgcggcgagg ccgtcgccgg
     1981 cgaacgcagg cggtgcgcct ggcattcgcg atgatcgcaa gacgactcca cccgcagacc
     2041 ccaatcgcgc gccgcctggc gcggacggag tagccccggt gagcggcgga gcgcgccctc
     2101 ggtcgagcga atccccgccg atatgacgtg gcagcggcac tattggcgac ccggccgcgg
     2161 atgtttcacg tcgacacgcc gagcgtcccc ccaacgaaag cgctctgcac ggtcgagagg
     2221 ctcccatgcg ctctcgagcg cgaccgtgct ttcgagcaag gtcgtttcac attattccgt
     2281 gcttcttcag cagcgcacgc aaaagggccg atgccaagac caagacgacc gcgatgacga
     2341 cgagcgcgag cagccagacc agcatgtgat gaccactcgg ttggcgacgc gggagcatga
     2401 gcgaacccga gcaaggcacg tgccgaaggc cggctcggcg gctggggcgg tggcggccag
     2461 ctgatcgtcg gcgacaggaa tgggggtacc ggcgagcccg aaggaccgcg agcaacgccc
     2521 gcgcgcactt ccgctccgac agcgctcgca gcaatcatcg ggcacttttg gtcgatgcgt
     2581 ggcactcccg actggcgacc agctgtccta tgctccgcgc catgttcttc caagtcgttt
     2641 cgcagttcaa aaggcagttg cagcagatcg agtcttggtt cgacgcagca caaaatttcg
     2701 ccaaagagcg agccttcgac ccaaacgttt ttgcatcgca gcgcttggcg cccgaccagt
     2761 tcgcgctcgc tcgtcaggtg cagatcgcct gtgacacggc gaaattcgca gtggcccggc
     2821 tgagcggcaa ggatgcgccg aaccaccccg atacggagca gacgctggac gagctccgtg
     2881 gccgcatccg ctccgtagtc ggctacctcg acggcttcag cgcgaaggac ttcgagggag
     2941 cagcaacgcg cacgatcagt cagccccgat gggaggggaa gaccatgacg ggcgccgact
     3001 acttcgtcga gcacgtcgtt ccgaacttcc atttccactt cacgcacgtt tacgccatcc
     3061 tgcgtcacaa cggcgtcccg ctcgggaaga aggattatct cggcgcgatc acgatgaagt
     3121 cgcccggctg aaccgccgag ttcggttcgg ctcttccaaa cgccgcgatg acgggacatc
     3181 gacgcccgtg atcgcggcgt tcgcgttttg ctatccgcgg ccgagcgaac gcgcgctcgc
     3241 gccgaagcgg atctcccggc gcgcgagctc ttccgtcgag aagccagggg cgggttcgaa
     3301 ctggtgggcg aagcgctccg ccaacgcgct aatcgtgagg cttgggttca cggcgaggtt
     3361 gccggggacg acgctgccgt cacagacgta caggccctcg taaccgaaca cttggtgacg
     3421 cgcgtcgatg acaccgttct cgggagcgtc accgatacag cagccaccca gaatatgggc
     3481 agtggcgggg gtccgcaaca tgacctcgga gatcacgttt tgcggctctc cgtgaatctc
     3541 ctcgccgaga atttcggcgg cttcctgcgc tacgggcaga tagctcggca caggcctttc
     3601 ggttggagct ccttcgagac cgaacaacgc gcgccgcaag atggagcggc caaggcgaag
     3661 acgcaggtgc tcatcgactg attgcatcac gagcaaaatg accgtactcc gagcccaatc
     3721 cgcgacgcgc cacaagcgga cgacgcgagg aagccggcgc aaaatacgac ccagcatctt
     3781 caacggacga gtgagcgcgc tcccgttcgg agtgatgggc actccgagca aacgcatcac
     3841 ccccgaaccg ctcgggtagc gcacgctttc gatcttggtg agtgaattcg gatgaaaggc
     3901 ggcaccgatg gcgatgccgc gtgagagctc ccggtgcgct tcgaagctgg tggcacccaa
     3961 caagctctcc ccgttcgtgc gcacgaagtc gccgaggcgc tccgagatgt tcggtaaagt
     4021 tcggtacaca tcgcggttcc tcagcagcag ctcgaccgtg ccgagcaccc ccgccgccac
     4081 gatgacgcgc cgagccttga aggcccgtcc cctcttgcgg aacgccgacg tgctcacctc
     4141 ggtttcgagc tcatagccgc cctcgacggg acgaatgcga gtcaccttgg tctccggaaa
     4201 gacttcggcg ccccacttct cggcaaagaa caggtagttc tggtcgagcg tgttcttcgc
     4261 tccgaagcga caccccacca tgcagccgcc gcagaagttg cacccgacgc gctcgggccc
     4321 tgcccccgaa aaataaggat ccggcacgcg ccgattgggt tcaccgaaga acacgcccac
     4381 ctcggtcgga tgaaacgtgg attcggcccc catccgctcg ctcacgcgcc gcagcgcctc
     4441 ttctccctcg aagagtgccg ggttcgtggt cactccgagc atacgccgcg cggtttcgta
     4501 atgcggtgcc agctctcgct tccagtcgac cgcgccccgc caggcggggt catcgaacac
     4561 gtgctcgtgg ggctgcatca gggtgttcgc gtagacgagg ctgccgccgc cgacgccgga
     4621 gccatgcaac accatcacgc ccttcaacag cgtgatgttc tggattccaa agcaccgcaa
     4681 acgcggagcc cacaggtact tggcgacgtt ccagttgctc ttcggaaaat cggtcgcgcg
     4741 atagcgctta ccggcctcga gcacggcgac gcgatagcct ttttgggcga gccgcatggc
     4801 ggacacactg ccgccgaatc cgctgccgac gatgacgaaa tcgaactctc gctctaaaga
     4861 ttccacgcgc ccgagtggtg agtatagctc aggtgtggtc gagcagctcg cgcacgaggt
     4921 cgagcaacgc ttggggcatg tacggcttgc tcagcaagcg cttcccaagt ctgccgaagt
     4981 cgggcggtaa cgcgctggcc gcgtagcccg tgatgaacag taccttcacg cccttgcgaa
     5041 tcgcctcgat gcgcgaaaat gcctgccagc cgtcgagccc gggcatgatc acgtcgagca
     5101 ggacgagcgc gacgtcggga ttcgactcga atacgtcgac ggccttgagg ccgtcttcgg
     5161 cttcgagcac gttgtaaccg gcgcgcttca gaattcggac cgcctgagca cgcacgagcg
     5221 gttcgtcctc ggcgaccagg atgagctcgt cgccgcgcgc gctgcctcgt cgaggggccg
     5281 ccttctcttc tggagcgcca atgatgaacg gcaagtcgac gcagaaagtg gatcctttac
     5341 cctgctcgct cttcaccgaa atgg
//
LOCUS       DASTKS010000931         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_818179, whole genome shotgun sequence.
ACCESSION   DASTKS010000931 DASTKS010000000
VERSION     DASTKS010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818179"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFQ35_19520"
     CDS             <1..761
                     /locus_tag="VFQ35_19520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9932907.1"
                     /translation="EMSAAMNDINGSAQQIAKIIKVIDEIAFQTNLLALNAAVEAARA
                     GRHGKGFAVVAQEVRSLAERSAKAAKETAELIESAVGKVSHGVRIAGATSTALEGILK
                     NVSKVVDLVGEIAAASDEQSKTIELVSQSMAQVTENAQSSSQQSTQVAAAAVEMSRQM
                     DVLRQRVETYKLAAPAAKPGVASADVLEQLSALLAARGISLTPKLLSDALSADAPNGH
                     ANGNGANGNGVLAAKLDPRVLLPLDNDERGFKGF"
     gene            778..2817
                     /locus_tag="VFQ35_19525"
     CDS             778..2817
                     /locus_tag="VFQ35_19525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9932908.1"
                     /translation="MSPEEHSPWHLFALDAEEVLQEIEESLLALESDPNSQSEINRLY
                     RGLHKLKGNSAVLELTQLEHLAHCAEDLTGLVRDHGVLLDDELCELMLVLVDCLKAEA
                     RAVGVRRGDADPDIVQPLLVRAERFIAERGLGQTLDVAPVPDGGFVIWSTPPSAAPPP
                     PAVDELGTLEIFATLVRETEPARNADLAALLARPSDAPARSRLLLSTADLRAAAERLE
                     LARVVRSLEGVEQALGDSSGAEHLKATEEELRAALAEVGLRKPDVALPAVLPAAPPAR
                     SQAPAPVKVAPKQSAGPSDRADYLRVDARKVAQIMDLAGEIGLACGAVTRHPALAGQD
                     LDGFSAAAHKLEVLIRELQNEVSAMRLVPVSGVFQNMRRVVRDAAKRTGKKVELELFG
                     EDIEMDKVLVDRLHDPLVHVMRNAVDHGIEKPEDRVRAGKPESGRLVLGATYQAGEVT
                     IQVRDDGGGLSREKILSRAQQRNLVPQGAELDDQQVAELVFMPGFSTKEQIDELSGRG
                     VGMDVVKTTIESLRGRVQLASQTGEGTRLTMIVPLTLAFLDAMVVRSDEHLFVLPIEK
                     IFEVFKAQPSGIAKASADGAVMVTVRGQLVPVLWLQRYYGEPDRAERLDDRILVVVQT
                     SRGKLALPVDEILGNHQVMLKPLRGMLAGVRAAAGCGMLSTGDVAIALDCEQLRA"
     gene            2810..3640
                     /locus_tag="VFQ35_19530"
     CDS             2810..3640
                     /locus_tag="VFQ35_19530"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9932909.1"
                     /translation="MPDAEFGIEQPLFATESGHAALHALRSWVQRTLGITYSDAQFDI
                     FADRIATLCRDLGSTPTALYERIQFGDVETNKRLAEAVSTNYTFFFRETESFDYIEQK
                     IFPRLPPGPLRFWSAATSSGEEAYSLAMLSRRYFGPDSSRIRVLGTDVSERQICAAEA
                     AVYPTGSLNEATLQRASAFFTTLDAGQVRVSEEIRGMCTFRRMNLLAFPWPFEQKFHL
                     ILLRNVLYYMSPEDGRRVLLAGARALAPQGYLTTSLTEPLFAPDYSLRQEAPSIYRQP
                     "
     gene            3637..4686
                     /gene="cheB"
                     /locus_tag="VFQ35_19535"
     CDS             3637..4686
                     /gene="cheB"
                     /locus_tag="VFQ35_19535"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HET9932910.1"
                     /translation="MTKRVRVLIVDDSALIRSVLQRGLSADPNIEVVGTARDPYQARD
                     LLVRTRPDVITLDLEMPRMDGLTFLRRFMAVLPVPTIVLSAVAGQSSALALEALAAGA
                     VEVVAKPSAHLKVGLEDMMSTLITKVKAAAQARVGRRIDDAKATAYRGKALDRTTDLV
                     IGMGASTGGVAALNRILPAFPASAPGIVIVQHIPAGFSTEFAQHLGTICAMKASEARD
                     GDRVLRGHILVAPGGDRQLEVRRVGGEYRVTLSKGPPVSGHIPSVNVLFNSLAEHVGR
                     NAAGCLLTGMGDDGATGLLSMLRAGGHTVAQDRETCAVWGMPAVAQSIGAVQRLLPLV
                     DIPAALLEWASAFAP"
     gene            complement(4775..>5327)
                     /locus_tag="VFQ35_19540"
     CDS             complement(4775..>5327)
                     /locus_tag="VFQ35_19540"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9932911.1"
                     /translation="PVFLLGHSAGGVVCSLYALDHSEEIAGVVCEDFAFELPAPDFAL
                     AVLKGVSHVAPHAHALTLKNSEFSRDAAVVDAMNGDPLIAHESQPFATAAAIVRADER
                     LKAEFRKIRTPIFIIHGAADKAAKPSGSEHFYERVGSPDKALKLYEHRYHDPLNDLGK
                     EEVMADILAWLGERTAQARNPTH"
BASE COUNT          954 a         1724 c         1749 g          900 t
ORIGIN      
        1 aagagatgag cgcggcgatg aacgacatca acgggtccgc ccagcagatc gccaagatca
       61 tcaaagtcat cgacgagatt gcgttccaga ccaacctgct cgccctgaac gcggccgtgg
      121 aagcggcgcg cgcgggacgc catggcaaag ggttcgccgt cgtcgcacag gaggtgcgga
      181 gcctcgccga gcgcagcgcc aaagccgcga aagagaccgc cgagctcatc gagagcgcag
      241 tgggcaaggt ttcacacggc gtgcgaatcg ctggtgcgac cagtacggcg ctggaaggga
      301 tcttgaagaa cgtctccaag gtggtggacc tcgtgggtga aatcgcggcc gcgagcgacg
      361 agcagtcgaa gacgatcgag ctcgtatcgc aatcgatggc gcaggtgacc gagaacgcgc
      421 agtcgagcag ccagcagagc acccaagtcg cggccgccgc ggtcgagatg agccgccaaa
      481 tggacgttct gcgtcagcgc gtcgaaacct acaagctggc cgcgcccgcg gcgaagccgg
      541 gggttgcctc cgccgacgtg ctcgagcagc tcagcgcgct gctcgcggcg agagggatat
      601 cgttgacgcc gaagctgttg agcgacgcat tgagcgcgga tgccccgaac ggccacgcga
      661 acggcaacgg cgcgaacggc aacggagtac tcgccgccaa gctggacccg cgcgtgctct
      721 taccgctcga caacgacgag cgcggcttca agggcttctg aacgggccga ggctgccatg
      781 tcgcccgagg aacattcgcc ctggcatctg ttcgccctcg atgcggaaga ggtgctccag
      841 gaaatcgaag agagtctgct cgcgctcgag agcgatccca actcccagtc cgagatcaat
      901 cgcctttacc gcgggctcca caagctcaaa ggaaactccg cggtgctcga gctcacgcag
      961 ctggagcacc tcgcacactg cgccgaagat ctgactggtc tggtccgtga ccacggagtc
     1021 ttgctcgacg acgagctctg cgagctgatg ctcgtgctcg tcgattgtct gaaggcggag
     1081 gctcgcgcgg taggagtacg ccgcggcgac gccgaccccg acatcgtaca acctttgctc
     1141 gtgagggccg agcgcttcat cgccgagcgg ggactcgggc aaacgctcga cgtggcgccc
     1201 gtccccgatg gcggcttcgt gatttggtcg acgccaccca gcgcagcgcc gccgccgccg
     1261 gcggtcgatg agctcggcac gctcgagatc ttcgcgacgc tggtgcgcga gaccgagccc
     1321 gcccgcaacg cagatctcgc ggcgctcctc gcgcgaccga gcgacgctcc tgcgcgctcg
     1381 cgactgttgc tgtccacggc cgacctgcgc gccgcagcag agcgactcga gctcgcccga
     1441 gtggttcggt cgctcgaggg agtcgagcag gcgctcggcg atagctccgg cgccgagcac
     1501 ctcaaggcga ccgaagagga gcttcgcgcc gcgctggccg aggtgggcct gcgcaagccg
     1561 gatgtggcgc tacccgcggt gctcccagca gctccccccg ctcgctcgca ggcgccggct
     1621 ccggtcaagg tcgcgcccaa gcaatcggcg gggccttcgg atcgagcgga ctaccttcgc
     1681 gtcgacgctc gcaaagtcgc tcaaatcatg gacttggccg gtgagatcgg cctcgcctgc
     1741 ggggccgtca cgcgacaccc ggccttggcg gggcaggact tggacggctt ctccgcggcg
     1801 gctcacaagc tcgaagttct gattcgcgag cttcagaacg aagtcagcgc catgcgcctg
     1861 gttcccgtct ctggcgtctt tcaaaacatg cgccgcgtcg tacgcgatgc cgccaagcgg
     1921 accgggaaga aggtggagct cgaactcttc ggtgaggaca tcgaaatgga caaggtgctg
     1981 gtggatcgac ttcacgatcc actggttcac gtcatgagaa acgcggtcga tcacggcata
     2041 gagaagcccg aagatcgcgt gcgagccgga aagcccgaaa gcggtcggct cgtgctggga
     2101 gcgacttacc aagccggaga agtcacgatc caggtgcggg acgacggagg cggcctatcg
     2161 cgagaaaaga tcctgagccg agcgcagcag cgaaacttgg ttccccaagg cgccgaactc
     2221 gatgaccagc aggttgcgga gctcgtgttc atgcccggct tctcgaccaa agagcagatc
     2281 gacgagctct cggggcgcgg agtgggcatg gacgtcgtaa agacgaccat cgaaagcctg
     2341 cgtggacggg tgcagcttgc gagtcagacc ggcgaaggca cgcggctcac gatgatcgtg
     2401 ccgctcacgc tggcgttctt ggacgcgatg gtggttcgct ccgacgagca cttgttcgtg
     2461 ctcccgatag agaaaatctt cgaggtgttc aaggcacagc cgtccggcat cgccaaggcg
     2521 tccgcggatg gcgcggtgat ggtgacggtg cgcgggcagc tcgttccggt gctgtggctg
     2581 cagcggtact acggggagcc cgatcgcgcg gaacgcctcg atgatcgcat cctggtcgtg
     2641 gttcagacgt cccgcggcaa gctcgcgttg cccgtggacg aaatcctcgg caatcaccag
     2701 gtcatgttga agcctctgcg cgggatgttg gctggcgtgc gcgccgccgc tggttgcggc
     2761 atgttgtcga cgggcgacgt ggccatcgcg ctcgactgtg agcagctgcg tgcctgatgc
     2821 cgaattcgga atagaacaac cactcttcgc gaccgagtcc ggacacgcgg cgctgcacgc
     2881 gctgcgctcg tgggtgcagc gtaccctcgg aatcacctac agtgacgcgc agttcgacat
     2941 cttcgcggac cgaatcgcga cgttgtgccg cgacctcggc agcacgccga cggcgctcta
     3001 cgaacgaatt cagttcggcg acgtcgagac caacaagcgg ctcgccgagg cggtatccac
     3061 caactacacc ttcttcttcc gcgagacaga gtcattcgac tacatcgagc agaagatctt
     3121 ccctcgcctg cccccagggc cattgcgctt ttggtcagca gcgacttcca gcggcgaaga
     3181 ggcgtacagc ctcgcgatgc tgagccgtcg ctactttggt cccgactcgt cgcgaatccg
     3241 cgtcctcgga acggacgtca gcgaacgtca gatctgcgca gcagaggcgg ccgtctaccc
     3301 cacgggctcg ctgaacgaag cgaccttgca gcgtgcgagc gcgttcttca ccactctcga
     3361 cgccgggcaa gtccgcgtga gcgaagagat ccgcggcatg tgtacgtttc ggcgcatgaa
     3421 cttgctcgcg ttcccgtggc cgttcgagca gaagtttcac cttatcttgc tgcgcaacgt
     3481 cctctactac atgagcccgg aggacggtcg tcgcgtcctc ctagcgggcg ctcgcgcact
     3541 tgcgccgcaa ggttacctga cgaccagctt gaccgagcct ctgtttgctc cggactattc
     3601 attgcgccag gaagcgccct cgatttaccg gcagccatga cgaaacgcgt gcgtgtgctc
     3661 atcgtcgacg actccgcgct catccgcagc gtcctgcaac gcgggctgtc ggcagatccg
     3721 aacatcgagg tagtggggac agcgcgagac ccctaccaag cgcgcgattt gctcgtgcga
     3781 acgcgccccg acgtaatcac gctggacttg gaaatgccgc gcatggacgg gctcacgttc
     3841 ttgcgccgat tcatggcggt gctgcccgta cccaccatcg tgctgtccgc ggtcgcgggg
     3901 cagagctcgg cgttggcgtt ggaggcactc gctgccggcg cggtcgaggt cgtggccaag
     3961 ccttcggctc atctcaaagt gggtctcgaa gacatgatgt ccacgctcat caccaaggtg
     4021 aaagctgccg cgcaggctcg cgtcggacgg cgcatcgacg acgcgaaggc caccgcctat
     4081 cgcggaaagg cgctcgatcg cacgacggat ctggtcatcg gcatgggcgc atccaccggc
     4141 ggggtcgcgg cgctgaaccg catcctgccg gcctttccgg cgagcgcgcc cggcatcgtg
     4201 atcgtgcagc acattccggc tgggttctcg accgagttcg cacaacattt gggcacgatc
     4261 tgcgcgatga aggcctccga ggcgcgtgat ggcgatcggg tgctgcgcgg acacattctc
     4321 gtcgcacccg gaggcgatcg tcagctcgag gtgcgacgcg tcggcggaga gtatcgcgtc
     4381 acactcagca agggcccgcc cgtctccggc cacattccct cggtaaacgt gctgttcaac
     4441 tcgctcgccg aacacgtggg ccgcaacgcc gccggctgtc tgctgaccgg tatgggcgac
     4501 gacggcgcga ccgggctgct ctcgatgctg cgggcgggcg gacacaccgt cgcgcaggat
     4561 cgggagacgt gtgcggtgtg gggcatgccc gccgtcgcgc aatcgattgg cgccgttcaa
     4621 cggctgctcc cactcgtcga cattccggcc gcgctgctcg aatgggcgtc ggcgttcgcc
     4681 ccctagccga aactcctgcg acggtcggat ccaaccgcca gcccccgagt cggcagatct
     4741 tgcgctgcgc ccgagcgaac gctcccgact tggatcagtg cgtcggattt cgggcctgcg
     4801 cggtccgctc gcccagccac gccaggatgt ccgccatcac ctcttcctta ccgagatcgt
     4861 tcagcggatc atggtaccgg tgctcgtaga gtttgagcgc cttgtcgggc gagccgactc
     4921 gctcgtaaaa gtgttcactt ccactcggct tggcagcttt gtccgcggcg ccgtggatta
     4981 tgaagatcgg ggtcctgatc ttgcgaaact cggctttgag acgctcgtcc gcgcggacga
     5041 tggccgcagc ggtggcaaaa ggctgcgatt cgtgggcgat gagcggatcg ccattcatcg
     5101 cgtccacgac cgccgcatcg cgtgagaact ccgaattctt cagtgtcagc gcgtgcgcgt
     5161 ggggagcgac gtgacttacc cctttgagca cggcgagcgc aaagtccggt gccggcagtt
     5221 cgaatgcaaa gtcttcgcag acgactcctg cgatttcctc cgagtgatcg agcgcgtaca
     5281 gggagcagac gactccgccc gcgctgtggc ccagcaagaa gacgggc
//
LOCUS       DASTKS010000932         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_818200, whole genome shotgun sequence.
ACCESSION   DASTKS010000932 DASTKS010000000
VERSION     DASTKS010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818200"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(629..>2407)
                     /locus_tag="VFQ35_19545"
     CDS             complement(629..>2407)
                     /locus_tag="VFQ35_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932912.1"
                     /translation="ACSAIGCVATSGTLSSLLFEVTPGISSDTNTTSRFFKRLDAIPF
                     VPTATNLGSAFKQGTLALELANPVTVSGSLNAAGCSIAFSDPSGAPIKSSSNGTIPAR
                     LTFIPSERMWGLAASTVMTTTSLRESGTLADYSFEVSLPAGAYDVYVEPFPVVDMPAG
                     QPSSCAVLPQLFRAESLNRSSSLKLALATPKHIDLKLRYAQSDQDLDGWRVDMIEPVT
                     GRLVSNTVELELPTRTDGTLVYAVPLDYVPVWGDDPKLTHGELVRLSPPKNTDAPTLF
                     FWREGLEWASPGEGVIDQLSAFPSAVLLSGSVSSGTVGVPASITFTANQVNGVPSGLF
                     PSFIRSVETDEEGDFQVNLVPGTYKAIAVPRNKQGERPFALSTTTLTVPAGSPVQAGA
                     TLPVGPAFSLQGNAFTSRGEGAWGAAVNADVSPSRITTSTFERAIGELPVVPRSASAT
                     IRDGTGSYQLFTEPGVYDLSVRPPGSSRYAWLVMPGFEPALGANDTLDISFPDLRLPA
                     PLPVELVVNWAGAKAPDPLLIAASVNAYALVDESGEPTTDARAAVSAVRIAEGRLDTD
                     GHVTLMLPAKLERPPEGFVSVPDSQP"
BASE COUNT          419 a          722 c          844 g          422 t
ORIGIN      
        1 gtggaaggcc cgtagccgct gcgacgaagt ggcggcaagt gtgaggagtc gaagttacga
       61 ggttgctcgc ggtgcgtcgc gaggttgctc gcgatggcga gtttgttggc tggggcaatc
      121 gcgccaattt tcgcctaaac agcgcccaac ggcggacact gctgctgagc ggcctcgtga
      181 gcggagcagc gtcagcggat gactggagca agcgaacgag ccgcgggcag agcgcggtac
      241 accccgcgct ttgcagcacg ctcgcgactc gggcacgaag ggcgcgtttc gggtcaaaaa
      301 actggcgagg cgcccctttc gcgtgagacc gaattccacg aaatccggcc tggatgcgct
      361 cgttccgcgc tcgacgctct cttcttcccg atgaacgacg ctcgaacgtc atggaaactc
      421 gcctttgagt ttgggatcga gcaggtccgc gtcgcagggt gaggcctcac gcggcctggt
      481 taccctcgcg agttccgcgg cctcgttacc ctcgcgagta ttctgcgcct cgttacctcg
      541 cgggtattct gcggctcgcg agtattctgc gcctcgttac cctcgcgggt attctgcaag
      601 ctgcggcctg gttgcttcgc gtggttcttt agggctgcga atcgggcacg ctgacgaagc
      661 cctcgggggg ccgctccaac ttggctggaa gcatgagcgt gacgtggccg tcagtgtcga
      721 ggcgcccctc ggcgatgcga accgcggaga ccgcagcgcg agcgtcggtc gtgggctcgc
      781 cgctctcgtc gaccagggcg taggcgttga ccgatgccgc gatgagcagc gggtccggag
      841 ccttcgcgcc cgcccaattg acgaccagct cgacgggcag cggcgctggc aaacgcaagt
      901 cggggaagct gatgtccaag gtgtcgttcg cgccgagcgc gggctcgaag ccgggcatga
      961 ccagccaggc gtagcgggag ctgcccggag ggcgaacgga tagatcgtag acgccgggtt
     1021 cggtgaagag ctgatagctg ccggtgccgt cgcggatggt cgcgctggcg gagcgcggaa
     1081 cgaccggcag ctcaccgatg gctcgctcga aggtgctggt cgtgattcgg gagggcgaaa
     1141 cgtcggcgtt cacggcggcc ccccaggcgc cctcgccgcg ggacgtgaac gcatttcctt
     1201 gaagtgagaa ggcaggccca acggggagcg tggcgcccgc ctgaacagga gacccggccg
     1261 ggacggtcaa ggtggtcgta gaaagcgcga atggacgttc gccttgcttg ttcctcggca
     1321 ctgcgatcgc cttgtacgtg cccggcacca aattgacctg gaagtcgccc tcctcgtcgg
     1381 tttcgacgct gcgaatgaag gacggaaaga gcccgctcgg cacaccgttc acttgattgg
     1441 ccgtaaacgt gatgctcgca ggaactccga cggtgccgga gctcacgctg ccgctgagca
     1501 agactgcact cgggaacgcg ctgagctgat cgatgacgcc ctcacccgga gacgcccact
     1561 cgagcccctc gcgccaaaag aacagcgtcg gggcgtcggt gtttttcggg ggagagagcc
     1621 gcacgagctc gccgtgggtg agcttcgggt cgtcgcccca aaccggcacg tagtcgagcg
     1681 ggacggcgta gacgagggtg ccgtcggtgc gcgtcggtag ttcgagctct acggtgttcg
     1741 acacgagtcg gcccgtgacc ggctcgatca tgtccacgcg ccagccatcg agatcctgat
     1801 cgctctgcgc gtagcggagc ttcaagtcga tgtgttttgg agtcgcgagc gcgagtttga
     1861 ggctgctgct tcgattcagg ctttctgcgc gaaagagctg gggcagcacc gcgcacgagc
     1921 tcggctgccc cgccggcata tcgacgacgg ggaatggctc cacgtaaacg tcgtacgcgc
     1981 ccgccggtag cgatacttcg aaggagtaat cggcgagcgt tccgctttcc cggaggctcg
     2041 tcgtggtcat gaccgtgctc gcggctaggc cccacatgcg ttcactcggt atgaaagtga
     2101 gacgcgcggg gatcgtgccg ttgctagagc tcttgatcgg agcgcccgag ggatccgaaa
     2161 acgcgatgct acaaccagcc gcgttgaggc taccggagac ggtgaccggg ttcgcgagct
     2221 cgagcgcgag cgtgccctgc ttgaatgcgg acccgaggtt cgtcgcggtg ggaacgaacg
     2281 gaatcgcgtc caagcgcttg aagaagcggg acgtcgtatt ggtgtccgag gaaatgccgg
     2341 gagtgacttc gaacaacaag ctcgaaagtg taccgctcgt ggcgacacag ccgatcgccg
     2401 agcaggc
//
LOCUS       DASTKS010000933         7211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1266037, whole genome shotgun sequence.
ACCESSION   DASTKS010000933 DASTKS010000000
VERSION     DASTKS010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266037"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            870..1310
                     /locus_tag="VFQ35_19550"
     CDS             870..1310
                     /locus_tag="VFQ35_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932913.1"
                     /translation="MLTRNQFCRASLAAFVAAAFTGLSAGSKADDAVQKVTAKLVEIP
                     SKFPPDDLYDYAYVMRYEVVGGPLDRSSILVAHYKPRQPRSKIKDKMKSFVSGKVKSF
                     AVGDVHKLELASDLKKIWKGPLVDEFAATDRKSIRYWCLVADPA"
     gene            1307..2377
                     /locus_tag="VFQ35_19555"
     CDS             1307..2377
                     /locus_tag="VFQ35_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932914.1"
                     /translation="MNDSSKARSVGARCAKSAFLLIAGALGLASAYPLFAEQRERSRV
                     DALLHGVATRAWGSTARNSARSAEDALSLRRERLGSLLSTWEAIGGCGAGSSSGAGAN
                     VKWIGRSVRGGLFSAQLMASYLRLPRQYNLSGGYSLSMVLQVTRDLGEKWNVGVAVPY
                     LYKYYRDYYSLPLDISNAGLGDVSLLLTRRLGAINDTFVTATLGIPTGKYDARFRNDL
                     LTQDKQLGHGRVSGSLMLDHVFDEVWGLIVVGGLASYRGGTNEFGNYRAPMGSVYGHA
                     GYYLGPFVPAMGLTFNAFAAHDRDRTLEQHSPLYTVAGSATVEWSNDYVALLLGGSFP
                     LRLPEFSTEPWIVAVGVAISPF"
     gene            2384..4363
                     /locus_tag="VFQ35_19560"
     CDS             2384..4363
                     /locus_tag="VFQ35_19560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9932915.1"
                     /translation="MTRVRASAGLSTNFGFAVLFCGVFCLCLTASTDGDVFWHLAAGR
                     EILKRGALLSVDVFSLSASGRPWIDVHWLFQVICAWGYALGGLRALVFGKAIIVASGA
                     LLPGQVVRRRAGAAALPLFIVVLFAALLAARDLLLLRPTIFTLLFLAAFIHTIEFARA
                     DGTPRRLWLLPLIQVAWVNVQGLFALGPSVIFAYWLGWELEARFGRASWFPFASAAGS
                     GSDGQMRRGLAFALASSLFACLVNPFGFRAVLLPTELLRRLTPGHANSFSNEIAENVP
                     PFILEQQTGQFWHLKWFLLALAVVVLVAGRRLRASHGILVGGFAFLALIANRNVLLLY
                     WVATPLAASYLFMGLRRRLPRIRSVHVSLRAATYASVLALSAVALGAQRREPSLDAPA
                     PFRVPELSARFIAAQGGTSRIFSADHFGGYLIWKLYPKHSPYIDTRLILRTPEQFGEY
                     LSVVDEPARFDAFAERERFDYVVLPTAYPERYLALIRHLYESEDWRLLVSDGTETLFG
                     RRDRAASPTMNLGDASTTARLLSDIDHRYVDPRVRADARLQLATLQLALGHADQAERA
                     LESSDDAQATALRARARLAQGDRAAAGRIAVRALEADPNQVRALNLLAVISIERGEIG
                     KAVGYLRRAARANPFDSETLTILRSLEEKPHDAIN"
     gene            4347..5513
                     /locus_tag="VFQ35_19565"
     CDS             4347..5513
                     /locus_tag="VFQ35_19565"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HET9932916.1"
                     /translation="MTRSTSATSSGRSCSRPRSRGWILCFALAACGVQEHLHEQVDTG
                     TGGTVFKPPPPGDGGSSTASGGTGPSANGGASNGGTSSASGGANQSSGGASDGRGGST
                     SGGANNASGGSSGNPARGGAAGAAMGGQSTASGGRSGVTVDINGTMLPKEDVIAFVHF
                     GHSNMFGYGSNPPGSKAYHFTDVNLRAWQYHGGKWSPALERTAGSGTTGAGPGTALMK
                     QAVDLAPSRYFVSLGYGVGSAYCSQFLRGGLYYDEFMAGPLALKGKVTFGAIVIMLGI
                     TERHGTDQDISNFPNCINDVVTSIRTDLGEPNLPLLLCDYEMGATGDLAPTAAFAQKI
                     IPKIREVPNVVSRSALVATDGLAMQDDHHFNLDGQKEWAKRALTIMKDKGWFLW"
     gene            5520..6977
                     /locus_tag="VFQ35_19570"
     CDS             5520..6977
                     /locus_tag="VFQ35_19570"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET9932917.1"
                     /translation="MVFSSHVFLFYFLPVVLVCYYAVPFRARTLLIAVASYVFYGWAN
                     PVWALIMFVGSSVDYVAGRLLLRLSGLPDDGALPPIITPDVPRTGRMKALLALSIATN
                     LGVLAVFKYTGFVAENVNSLARLFGARDAVVPVIHLILPVGISFYTFKAMSYVIDVYR
                     GEARPMRRFTDYMCFEAFFPDLVAGPIIRYAALEEQMRVRQHTLDKFARGVAFFAFGM
                     AKKILIANPMGNIADTAFDAGTLHAVDAWFGLVGYAFQIYFDFSGYSDMAIGLALMLG
                     FVLIQNFDSPYKSESITEFWRRWHISLSTWLRDYLYVPLGGNRRGPLRTYINLMTVML
                     VGGLWHGASWNFVIWGGIHGLMLAFERSQGKSSPYHRLPRALRVALTFAIVCLSWVFF
                     RADTLTSAVAYFRSLFGVSGAGDAAHAVAGALYTPYHLAVFLVAGIVVWAAPNTWAFT
                     SRLTPFRAAYGIAVLGMAVLLMWTQTVNPFLYFQF"
     gene            6982..>7211
                     /locus_tag="VFQ35_19575"
     CDS             6982..>7211
                     /locus_tag="VFQ35_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932918.1"
                     /translation="MTSPSNTQRGGPTDPSHDERLRRGILVTDIRRGVAWALVAAFIG
                     LIFAVPLGQLALERVRDEESVLKSLFQEVPTK"
BASE COUNT         1152 a         2272 c         2317 g         1470 t
ORIGIN      
        1 gggcggctat tctccgccga atagtcaaaa ccgagaggta acgatggagt tcgaggtgcg
       61 gcgcccaccc aagaacgcgg ccgtcgacaa caagggtttg tgggatcgag gttgcatggt
      121 ccaccactcc cgcctgcgtg atgtgacgag cattccgaag tgcgatggcg gcaggcaggt
      181 tcagggctgg tccgaacttg tgccaagcgc gtctgctcat cgcggacagg tcatcgcttg
      241 cggggacccc tgcgcgaggg tattgccgca atcaccgccg cgggttgttc acgagtcggc
      301 cccggtgatc cccgacgaaa aaccttcgca ggtttgctgc agcagcgctt ttgccccact
      361 gaattgtcgg ttcgaagagc ctaccactcg ccctcgaatg gctcgactgc cgccgcgacg
      421 agtcgaggcg ttgctgcaac gtgcctgcgc tcggcaaaag cgtcgtggcg cgccgcacgc
      481 tcgacacgac cgagagcaac tgggcacccg gttggatcct gagagacgca aactgtgcgc
      541 gcggtgaggg tcggcggcgc gcaaaattgg gccgtggcgg ggtagtatcc ggacccgcag
      601 ctttgcaggc agtcaatcgt cgcgacgcag cccagcgtgg cgccacggcg cgaatgggcc
      661 ggaaatgcgc cataaaatcg aggatgcggc tccgaattcg tcgtcgcgct ttgacggatc
      721 gctcaacgag cgcacactgc catccacgaa gcgtggctag ccacgcgtat cgttagaaaa
      781 ttccaggttc gagcgcgctg atcgcaccgt ccgaagatgg ctgcggtcag ctctgtgtgt
      841 atttagaaat gcctcaacgg agtcgctgct tgttaactcg caatcagttt tgccgcgcct
      901 cgcttgctgc gttcgtcgcg gcagcgttca ctggtttgtc agctggttcg aaggccgacg
      961 atgcagtcca gaaggtgacc gcgaagctcg tagaaattcc gtcgaaattc ccgccggacg
     1021 acctgtacga ctacgcgtac gtcatgcgct atgaggtcgt gggcggtccg ttggatcggt
     1081 cgtccatcct ggtcgcgcac tacaagccgc gccagccacg ctcgaagatc aaagacaaga
     1141 tgaagtcgtt cgtcagcggc aaggtgaaaa gctttgcagt cggtgacgta cacaagctcg
     1201 agctcgcgag cgacctgaag aagatctgga aggggccgct ggtcgacgaa tttgcggcga
     1261 cagatcggaa gagcatccgc tactggtgtc tggtggcgga tcctgcatga atgactcgtc
     1321 gaaggcgcgc agtgtcggtg ctcgttgcgc caagtcagcg ttcctattga ttgccggcgc
     1381 gctcggtttg gcttcggcgt acccgctgtt tgccgagcag cgtgagcgaa gccgcgtcga
     1441 cgcattgctg cacggcgtgg caacgcgcgc ttggggctcg actgcgcgga actctgcgcg
     1501 aagtgcagag gacgccttga gcctgcggcg cgaacggctc gggtcgctct tgtcgacctg
     1561 ggaagccatc ggtggctgcg gcgcgggtag ctcttcgggc gccggtgcga acgtgaagtg
     1621 gatcggccgc agcgtgcggg gcgggctctt ctcggcgcag ctcatggcga gctatttgag
     1681 gttgccgcgg caatacaatc tgtccggcgg gtactctcta tcaatggtcc tgcaggtcac
     1741 ccgcgacctt ggtgagaaat ggaacgtcgg cgtcgcggtg ccctacctct acaagtacta
     1801 ccgcgactac tactcgctcc ctctcgacat ctcgaacgcg ggcctcggtg acgtcagctt
     1861 gctgctgacc cgccggctgg gagcgatcaa cgacaccttc gtcaccgcaa cgctcggcat
     1921 acctaccggc aagtacgatg cgcgcttccg caacgacttg ctgacccaag acaagcagct
     1981 cgggcatggt cgagtctccg gctcgctgat gctcgaccat gtcttcgacg aggtatgggg
     2041 gctcatcgtg gtcggggggc ttgcgagcta tcgcggcggc acgaacgagt tcggtaatta
     2101 ccgcgcgccg atgggcagcg tgtacggaca cgctggctac tacctgggac cgttcgttcc
     2161 tgcgatgggg ctaacgttca acgctttcgc agcgcacgat cgcgaccgca cactggagca
     2221 acattcgccg ctctacacgg tagccggtag cgctaccgtg gagtggtcga acgactatgt
     2281 cgctctcttg ctcggcggct cgttcccgct gcgcctaccc gagttttcga cggagccgtg
     2341 gatcgtggcg gtcggcgtcg ccatctctcc cttctgagaa gcgatgacgc gagttcgcgc
     2401 ttccgccggc ctttcgacga acttcgggtt cgcggtgctg ttctgcggcg tcttctgtct
     2461 ttgcttgacc gcttcgaccg acggcgacgt attctggcac ctcgccgcgg ggcgcgagat
     2521 cttgaagcgc ggcgcgctgc tttcggtgga cgtgttttcg ctcagcgcca gcggccgccc
     2581 atggatcgat gttcactggc tcttccaagt gatttgcgcg tggggctacg cgctcggtgg
     2641 cttgcgcgcg ctcgtgttcg gcaaagcgat catcgttgcg agcggagcac tgttgcccgg
     2701 acaggtggtg cgtcgacgcg ctggtgccgc ggctctcccg ctcttcatcg tggtgctgtt
     2761 tgcggccttg ctcgcggcgc gcgacttgct tctgctgcgc ccgacgatct tcaccctgct
     2821 gttcttggcg gcgttcatcc acacgatcga gtttgcgcgc gcggacggta ctccgcgtcg
     2881 gctctggctc ttgccgctga ttcaagtcgc gtgggtcaac gtgcaagggc tcttcgcgct
     2941 aggccctagc gtcatttttg cgtattggct cggttgggag ctcgaggctc ggtttggacg
     3001 cgcaagctgg tttccgtttg cgagcgctgc cgggagtggt tccgacggac aaatgcggcg
     3061 cggtctcgcg tttgctctcg caagctcgct cttcgcatgt ctcgtgaacc ctttcgggtt
     3121 tcgcgccgtg ctcctgccga cggagctact ccgacggctg acgcccgggc acgccaactc
     3181 attctcgaac gagatcgccg aaaacgtgcc gccgttcatt ctggagcagc agacgggaca
     3241 gttctggcac ttgaagtggt tcctgctcgc cctggccgtg gtcgtcctcg tcgccgggcg
     3301 gcgcttgcgg gcgtctcacg gcattctcgt cggtgggttc gctttcctcg cgttgatcgc
     3361 caaccgcaac gtcctcttgc tgtattgggt cgcgaccccg ctcgcggcaa gctacctctt
     3421 catgggattg cgccggcgct tgccgcgaat tcggtcggtg cacgtcagcc tccgtgccgc
     3481 gacttacgca agcgtgctcg ccctttcggc ggtggcgctc ggcgcgcaac gccgcgagcc
     3541 gtctctcgat gcgccggcgc cgttccgcgt gccagagctg tcggcgcgct tcatcgcggc
     3601 gcaaggcggg acgagccgca tattctctgc cgaccatttc gggggctacc tcatctggaa
     3661 gctttacccg aagcattcgc cgtacatcga cacgcggctc atcttgcgga ctcccgagca
     3721 attcggcgag tacctgtcgg tcgtcgatga gccggctcgt ttcgatgcgt tcgccgagcg
     3781 cgagcgcttc gactacgtcg tgttgcctac ggcgtacccc gagcgttacc tcgccctaat
     3841 tcggcacctg tacgaaagtg aagactggcg gctcttggtc agtgacggga cagaaacgct
     3901 cttcgggcgg cgcgaccgcg ccgcgtcgcc gacaatgaac ctcggcgacg cgagcacgac
     3961 ggctcgattg ctctccgaca tcgaccaccg ctacgtcgac ccgcgggtac gcgccgacgc
     4021 acgcttgcag ctcgcaaccc ttcagctcgc gctgggccac gccgatcagg ccgagcgagc
     4081 gttggagtcg agcgacgatg cgcaggcgac cgcgcttcgc gcccgcgcgc gtctcgccca
     4141 aggagatcgc gctgccgcgg gccggatcgc tgtcagggcg ctcgaagccg atcccaatca
     4201 agtccgtgcc ctcaacctcc tcgcggtgat ttcgatcgag cgcggggaga ttggaaaagc
     4261 cgtgggctat ttgcggcgcg cggcgcgcgc gaaccctttc gattccgaaa cgctcaccat
     4321 tctccgtagc ctggaggaga agccacatga cgcgatcaac tagcgccacg agttccggcc
     4381 gttcgtgttc acgcccgcgc tcgcgggggt ggatcttgtg cttcgcactc gccgcgtgcg
     4441 gcgttcagga gcaccttcac gagcaagtcg atacgggcac tggaggcact gttttcaagc
     4501 cgccgccccc tggggacggg ggctccagca ccgcgagcgg cgggaccggg ccttcggcga
     4561 acgggggcgc ttcgaacggc ggaacatcga gcgctagcgg gggcgccaac cagagcagtg
     4621 gtggcgcgtc ggacggtcgc ggcggctcga cgtcgggcgg ggccaacaac gcgagcggcg
     4681 ggtcttccgg caacccagcg cgcggaggtg cggcgggcgc ggcgatgggc ggtcagagca
     4741 cggctagcgg cgggcgctcc ggagtgaccg tggacatcaa tgggacgatg ctgccgaagg
     4801 aagacgtgat cgctttcgtg cacttcgggc actcgaacat gttcggttat ggcagcaacc
     4861 cgcccggttc gaaggcgtat cacttcaccg acgtcaatct gagagcctgg cagtaccacg
     4921 gtggcaagtg gtcgcctgct ctcgagcgca ccgccggctc cggcactacc ggtgcgggac
     4981 cgggtacggc gttgatgaag caagccgtag acctcgcgcc tagccgctac ttcgtatcgc
     5041 tcggatacgg agtcggtagc gcctactgct cgcaattcct gcgtggcggg ctctattacg
     5101 acgagttcat ggccggcccg ctcgcgttga agggcaaggt cacgttcgga gccatcgtca
     5161 tcatgctcgg catcaccgag cgtcacggca cggatcagga catctcgaac ttcccgaact
     5221 gcatcaacga tgtcgtgacc tcgatccgta ccgacctcgg cgagcccaat ctaccgcttc
     5281 tgctctgcga ctacgaaatg ggcgcgaccg gcgacctcgc tcccaccgcc gccttcgcgc
     5341 aaaagatcat tcccaaaatc cgcgaagtgc cgaacgtcgt ctcgcgctcg gcgctcgttg
     5401 cgactgacgg gcttgcgatg caggacgacc accacttcaa cctcgatggg cagaaggaat
     5461 gggcgaagcg cgcgctcacg atcatgaagg acaagggctg gtttctctgg tgagtccgta
     5521 tggttttttc gagccacgtc ttcctattct actttttacc agtcgtactg gtttgctact
     5581 acgcggtccc gttcagggcg cgcactctgc tcatcgcggt tgcaagttac gtcttttacg
     5641 gttgggcgaa cccggtttgg gcgctcatca tgttcgtggg gtcgagcgtc gactacgtcg
     5701 ccggtcggct cttgctgagg ctctcgggcc ttcctgacga cggcgcgctt ccgcctatca
     5761 tcacgccaga cgtcccgcgc acgggacgaa tgaaggcgct gctcgcgctc tccatcgcaa
     5821 cgaatctcgg cgtgctcgcc gtattcaagt acaccggctt cgtcgccgag aacgtgaact
     5881 ctctcgctcg actgttcggc gcgcgcgacg cggtcgtgcc ggtgattcac ctgattttgc
     5941 cggtcggcat ctcgttttac acgttcaaag ccatgagtta cgtcatcgac gtctatcgcg
     6001 gcgaagcgcg cccgatgcgt cggttcacgg attacatgtg cttcgaggcg ttctttccgg
     6061 atctcgtggc gggccccatc attcgctacg cggcgctcga agagcagatg cgggtgcgcc
     6121 agcatacgct cgacaagttc gcgcgcgggg tcgcattctt cgcgttcggg atggcgaaga
     6181 agatcctcat cgcgaacccg atggggaata tcgccgacac ggcattcgat gccggcacgc
     6241 tccacgcggt cgacgcgtgg ttcggcctgg tcgggtacgc gttccagatc tacttcgact
     6301 tctcgggata ctccgacatg gcgatcggac ttgcgctgat gctggggttc gtcctgattc
     6361 agaacttcga ctcaccttac aagtcggaga gcatcaccga gttttggagg cgctggcaca
     6421 tcagcctctc gacctggctg cgcgattacc tgtacgtccc gctcggtggg aaccggaggg
     6481 ggccgctgcg cacgtacatc aacttgatga cggtcatgtt ggtgggcggg ctctggcacg
     6541 gcgctagttg gaatttcgtc atttggggcg gaatccacgg cctgatgctc gcgttcgagc
     6601 gctcccaggg caagtccagc ccgtaccacc ggttgccgcg cgcgctgcgg gtggcgctca
     6661 ccttcgctat cgtctgcctt tcgtgggtgt tcttccgcgc cgacacgctg acgagcgccg
     6721 tcgcgtactt tcgctcgctc ttcggagtgt cgggggcggg agatgcggcg cacgcggtgg
     6781 ccggggcgct ctacacgccg tatcacctcg cggttttcct cgtcgctggc atcgtcgtgt
     6841 gggccgcgcc gaatacatgg gcattcacga gtcgcctcac gccgttccgc gccgcctacg
     6901 gcatcgcggt gctagggatg gccgtgctgc tcatgtggac gcaaacggtc aacccgttct
     6961 tgtacttcca gttttgagcc gatgacttca cccagcaata cgcagcgcgg tgggcctacg
     7021 gatccttcac acgatgagcg attgcgtcgc ggcatcctcg tcaccgatat ccgtcgcggc
     7081 gtcgcctggg cgctggtagc cgctttcatc ggcttgatct tcgctgttcc cttggggcag
     7141 ctcgcgctgg agcgagttcg agacgaagag tccgtgctca agtcgctctt ccaggaggtc
     7201 cccacgaaag a
//
LOCUS       DASTKS010000934         5177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1712572, whole genome shotgun sequence.
ACCESSION   DASTKS010000934 DASTKS010000000
VERSION     DASTKS010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712572"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFQ35_19580"
     CDS             complement(<1..948)
                     /locus_tag="VFQ35_19580"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9932919.1"
                     /translation="MSVAPGSSRESLRRLSKQTEAGLVELERVARVIEANVLRYGDIL
                     TRPHPLILCVRRGNLACLVTSAKVWDRARDLLAPFTSSLAHQEARVLLLGTPLDPDLS
                     RALDQGVAALLGESPSVSELCVALHNAFELMELKTRSESRGKWLSRYRFELGELIEIA
                     RALTTEREIDKLLSLILEKARFVTGADAGSLYVVEGDEPDILRRKLHFKLSQNDSLSF
                     DSREFVMPINARSMAGYVALEKTTLSIADVYDMPASSPFGFDRSFDERVGYRSKSMLV
                     TPLTSSLGEVFGVFQLFIKMREPSKMLLSPAEVETEVVAF"
     gene            complement(945..1970)
                     /locus_tag="VFQ35_19585"
     CDS             complement(945..1970)
                     /locus_tag="VFQ35_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9932920.1"
                     /translation="MAESPYSTNIEVGDTQVLMALAGPHSALLTEVARETQTEVSLRG
                     NTIYLSGDEPDVELACRFLTDAVALVERGYTLTPNDVPGSVRGLRADPNRSLTELLDE
                     TILITPKKRPIVPKSIGQRRYVEAIRSHDLTFGIGPAGTGKTYLAMAMAASWLMQKRV
                     KRIVLARPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALYDMLDFERAEQLRERGQVEV
                     APLAFMRGRTLNDAFVILDEAQNATSEQMRMFLTRFGFGSKVVVTGDITQTDLPSGAR
                     SGLREARDLLQNVQGISFCSFTDQDVVRHPLVQKIIVAYEKRDEALREQKNNERAQRK
                     LESASEK"
     gene            2086..3333
                     /locus_tag="VFQ35_19590"
     CDS             2086..3333
                     /locus_tag="VFQ35_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9932921.1"
                     /translation="MARLARWIGVGAAAGIILALVPVVRGGFTPLSQPLRKLVRPSAA
                     PAPNPIPKLDGIDLLRMDIRPQRVTAPLPEGRTAELTLDPVVQRAARAQMQRYRIPEA
                     GTVLIEVKTGKILTYASYVADGEPFDVNVRAGAPAASVFKVVTGAALIEKAGLNAETE
                     QCYHGGKSRILPDELVDDPRRDRWCATVAIAMGRSLNVVFGKLAQKHLTPEDVASMGG
                     ALGFGAPVPFVVQNEAPKIQIPQEPVEFARTSAGFWNTTLSPLAAASLAQTIANGGVT
                     LEPRIVSAVFQGRDTVWKDEREPRPLRQAIKSTTASELTLMMSQTVSNGSAFKSFHDE
                     KGNSYLPGIEVAGKTGTLSDHEANRHYTWFIGFAPAEKPEVAVSSLIVNTPTWQIKAA
                     QLAREVLRAYFARRGAKGVTPPS"
     gene            3574..>5177
                     /locus_tag="VFQ35_19595"
     CDS             3574..>5177
                     /locus_tag="VFQ35_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932922.1"
                     /translation="MSKELSNVGPELARDGKGEIRGRQLGPVDRGRRHTRTNLALALL
                     GLVVSCGMHTASDSSRSADNNGAEPTGGSFSGGLNPGAGGATVNVPPLPPEKEKESTF
                     KLPVRSGRWVWSANPLSNRVAVIDTKDLGVRVASAGFGPTYLAPLPASATVDSAAIVL
                     NARSSDASVMRLVDGTIDVKTVPTHVGANACSVSPSGNWAIAWSDAASVTSPDPADSY
                     QDITLIDVSGAAPRATRLSVGYRPNRIFFDRSEAHAFVVSEASVSVIDLSRDAPNVSR
                     DITLGTGREKPTDVAVVPQGTHALFRVEGSPNVSIVELESGARTNIVLSGPVTDLDLV
                     ADGASAIAVVRGRPANVGDASNASGGGSSGSANGSGGNVSRVELNGGAGGGAGEGQNP
                     DALAGGASGGEAAGNGETDAGAGGVAGEGGQGTPPASGYGPSEVAILSVARVLESPME
                     FSRVQVPDVVGSVAVSSDGAESVLFTTAASFDRVTVLPTDPTSSAFLTPRSVYVHAPV
                     DAVFIAPDHTHALVTLKATQGSSGFG"
BASE COUNT          909 a         1649 c         1711 g          908 t
ORIGIN      
        1 gaaggcgacg acttcagtct cgacctcggc tggggagagc agcatcttgc tcggttcgcg
       61 catcttgatg aagagctgaa agaccccgaa gacctcaccc agactcgagg tgagcggcgt
      121 gaccaacatg ctcttcgagc ggtagcctac gcgctcgtcg aacgaacgat cgaagccgaa
      181 cggtgagctc gccggcatgt cgtacacgtc ggcgatgctg agcgtcgtct tctcgagcgc
      241 gacgtagccg gccatgctgc gcgcgttgat cggcatcacg aattcgcgcg agtcgaacga
      301 gagcgagtcg ttttggctca gcttgaagtg gagcttccgg cgcaagatgt cgggctcgtc
      361 accctcgacg acgtacaagc tgcccgcatc ggcgccggtg acgaagcgcg ccttctccaa
      421 aatgagcgaa agcagcttgt cgatttcgcg ctcggtggtc agcgcgcgtg cgatctcgat
      481 gagctccccg agctcgaagc gatagcgcga cagccatttc ccgcggctct cgctgcgggt
      541 cttgagctcc atcagctcga atgcgttgtg cagcgcgacg cacagctccg aaaccgaagg
      601 gctctcgccg agcaacgccg cgaccccttg atccagcgcg cgcgaaaggt ccggatcgag
      661 cggcgtgccg agcaacagca cccgcgcctc ttggtgtgcg agcgagctcg tgaacggcgc
      721 gagcaagtct cgcgcgcgat cccagacctt cgcgctcgtg acgaggcacg cgaggttacc
      781 gcggcggaca cagaggatga gcgggtgcgg ccgcgtgagg atgtctccgt aacgcagcac
      841 gttggcttcg atgacacgtg cgacgcgctc gagctcgacg agccccgcct ccgtctgctt
      901 ggaaagccga cgtagcgatt ctctgctcga gccgggcgcg acgctcattt ctccgaggcg
      961 gactcgagct ttcgctgtgc gcgctcgttg ttcttctgct cgcgcagggc ttcgtcgcgc
     1021 ttttcgtaag cgacgatgat cttctgcacg agagggtgac gcaccacgtc ttggtccgtg
     1081 aacgaacaga atgaaatacc ctggacgttc tgcaagagat cacgcgcctc gcgcaggccg
     1141 ctgcgcgcgc cggagggtaa atccgtctgg gtgatgtcac ccgtcacgac caccttggag
     1201 ccaaagccga agcgtgtgag gaacatgcgc atctgctcgc tggtcgcgtt ttgcgcttcg
     1261 tccaaaatca cgaaagcatc gttcagagtg cggccacgca tgaaggcgag cggagccact
     1321 tccacctgac cgcgctcccg cagctgctcc gcgcgctcga agtcgagcat gtcgtagagc
     1381 gcgtcgtaga gtgggcgcag gtacgggttc accttctctg ccaagtcacc cggcaaaaat
     1441 cccagctttt cgccagcttc gaccgcggga cgcgcgagaa cgatacgctt cacgcgcttc
     1501 tgcatcagcc acgaagccgc catcgccatc gcgaggtagg tctttccggt gccggcgggg
     1561 ccgatcccga acgtgaggtc gtggctccga attgcttcga cgtagcgccg ctgcccaatc
     1621 gacttgggaa cgatgggccg cttcttcggc gttatgagga tcgtctcgtc gagcagctcg
     1681 gtgaggctcc gattgggatc ggcgcggagt ccgcgcaccg agccggggac gtcgttgggg
     1741 gtgagggtgt agccgcgctc cacgagcgcg accgcgtcgg tcaagaatcg gcacgcgagc
     1801 tcgacgtcgg gctcgtcgcc cgagaggtag atggtgttgc cccgcaagct cacttcggtc
     1861 tgggtctctc gtgcgacctc cgtcagcaag gccgaatgcg gcccggccaa cgccatgagt
     1921 acctgggtgt ctccgacttc gatgttcgtt gaatacgggc tttcagccat gcaggtcgcc
     1981 tgacgggcag catagaacga ttcgggctcg ccgccgcccg ggcagcgggc cttttggtcc
     2041 ggtttcttgg cccggaagct cgccagccga taaccgggaa ccgtaatggc ccggctagct
     2101 cgatggatcg gcgtgggtgc cgcggcggga atcatcctgg ctttggttcc cgtcgtacgc
     2161 ggtggcttca ctccgctcag ccaaccgctg cgtaaattgg tacgtccgag cgctgcgccc
     2221 gctccaaatc cgatcccgaa gctcgatggg atcgaccttc tgcggatgga tattcgaccc
     2281 cagcgggtga cggccccatt gcccgaggga cgcactgcgg agctcaccct cgaccccgtc
     2341 gtgcagcggg ccgctcgtgc acagatgcag cgctaccgga ttccggaagc gggcacggtc
     2401 ctcatcgaag tgaagaccgg gaagattctc acgtacgcga gctacgtcgc cgatggcgaa
     2461 cccttcgacg tgaacgtccg cgccggcgcg cccgccgcga gcgtcttcaa ggtggtgacc
     2521 ggcgcggcgc tcatcgaaaa ggccgggctc aacgccgaaa ccgagcaatg ttaccacggc
     2581 ggcaagagcc gaatcctgcc cgatgagctc gtcgacgacc cgcggcgcga ccgttggtgc
     2641 gcgacggtcg cgatcgcaat gggccgcagc ttgaacgtcg tctttgggaa gctcgctcaa
     2701 aagcatctca cgcccgaaga cgtcgcttcg atgggcggcg cgctcggctt cggtgccccc
     2761 gtgccgttcg tcgtccaaaa cgaagctccc aaaattcaaa tcccccaaga gccggtcgag
     2821 tttgctcgca cttcggcggg cttttggaac acgactcttt cgccgctcgc ggcggcgtcg
     2881 ctcgcgcaaa cgatcgcgaa tggcggcgtg actctggagc cacgcatcgt ctcggccgtt
     2941 ttccaaggaa gagacaccgt ttggaaggac gaacgcgaac ctcgccccct gcgtcaagcc
     3001 atcaagagca cgaccgcgag cgagctcacg ttgatgatgt ctcaaacggt ttcgaacggc
     3061 tccgccttca agtcgtttca cgatgagaag ggcaactcgt atctgccggg catcgaggtc
     3121 gccggaaaga ccggcacgtt gagcgaccac gaggccaacc gccattacac ctggttcatc
     3181 ggcttcgctc cagccgagaa gccggaagtc gccgttagct cgctcatcgt caatacgccc
     3241 acgtggcaga tcaaagccgc tcaactcgca cgcgaagttt tgcgcgcgta ttttgcgcgg
     3301 cgaggggcca agggcgttac gcctccgtcc tgaggcctcg ctgaaggtct cggcgctctc
     3361 gcgaccgact catcggtgcg caggtgctga gctcgagcgc gggcgcgcgg cgaccctgac
     3421 acgaatgtca ggctgacgtg cgtgcggcac aaccgacgcc agcgcgacgt gaggcgacga
     3481 ggccgagttc gacgccgatt tcggtattcg tattcatggc ccgtcgcttg cttcgcacgg
     3541 cgcggctgtg ttcgaggacc gatcgggcgg cgcatgtcga aagagctatc gaacgttgga
     3601 cccgagctcg ctcgggatgg caaaggcgag attcggggcc ggcagctcgg gccggtagac
     3661 cgcggtcggc gacatacgcg gacgaatctg gcgttggcgc tgctcgggct ggtcgtgagt
     3721 tgcggcatgc acaccgcttc cgacagctcg cggagcgccg ataacaatgg cgccgaaccg
     3781 acgggaggct cgttttcggg cggcctcaac ccgggcgcgg ggggcgcgac cgtcaacgtg
     3841 ccgccgctcc ctccagaaaa ggagaaggag agcaccttca agctcccggt gcgcagcggg
     3901 cgttgggttt ggtcagcgaa tccgttgagc aaccgcgtcg cggtgatcga cacgaaggat
     3961 ctcggcgtgc gcgttgcgtc ggcagggttc ggtcccactt atctcgcgcc actgccggcc
     4021 agtgcgaccg tcgacagcgc ggccatcgtg ttgaacgcgc gcagcagcga cgcgagcgtg
     4081 atgcgcctcg tcgacggaac gatcgacgta aagaccgttc ccacgcacgt cggcgcgaat
     4141 gcgtgcagcg tgagtccgag cggcaattgg gccatcgctt ggagcgatgc ggcttcggtc
     4201 acctcgcccg atccggccga cagctaccaa gacatcaccc tcatcgacgt ttccggggct
     4261 gcaccgcgcg cgacacgcct cagcgtgggc taccgcccga atcgaatctt cttcgatcgc
     4321 agcgaagccc acgcgttcgt cgtttcggag gcttcggtca gcgtgatcga tttgtcgcgt
     4381 gatgcgccga atgtcagccg cgacatcacg ctcggcaccg gtcgagagaa gccgaccgac
     4441 gtcgcggtcg tgcctcaggg cactcacgcg ctgttccgtg tcgaggggag ccccaatgtg
     4501 agcatcgtcg agctcgagag cggcgctcgc acgaacatcg tgctctctgg gccagtcact
     4561 gatttggatc tcgtcgcgga cggagcctcg gcgatagccg tagtgcgcgg tcgtcccgcg
     4621 aatgttggag acgcatcgaa cgcgagcggg ggcggttcat cgggcagcgc gaacgggagc
     4681 ggcggaaacg tctcgagagt cgaactcaac ggcggagcag gcggcggcgc tggagagggc
     4741 cagaatccgg acgccttggc aggaggcgcc tccgggggtg aggcagcggg caacggggaa
     4801 acggacgcgg gcgcgggagg cgtggctggg gaaggcggac aaggcacgcc acccgcgtcc
     4861 gggtacggtc cttccgaagt cgcgattctc tccgtcgcgc gcgtcctcga atcgccgatg
     4921 gagttttctc gagtgcaggt gccagacgtc gtcggcagtg tcgcggtgtc gagcgatggc
     4981 gcggagtcgg tgctgttcac gaccgccgca tcgttcgacc gtgtaacggt gttgcccacg
     5041 gatccgacga gctctgcgtt tttgacgccg cgctcggttt acgtgcacgc gccggtcgac
     5101 gcggtcttca tcgcgcccga tcacacccac gcgctcgtga ctctcaaggc gacccaagga
     5161 agcagcggct tcggcat
//
LOCUS       DASTKS010000935         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1490462, whole genome shotgun sequence.
ACCESSION   DASTKS010000935 DASTKS010000000
VERSION     DASTKS010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490462"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..464)
                     /locus_tag="VFQ35_19600"
     CDS             complement(138..464)
                     /locus_tag="VFQ35_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932923.1"
                     /translation="MREVVQCLLTKHSEALVELQELRLAVDEILKTLADLDRIAQSLA
                     TDVERVRRSSVGPVPIVLAVHDRVTALSSLTSQVLAAFRELERGIKGWEVGNAGSPTS
                     NGTAVN"
     gene            659..961
                     /locus_tag="VFQ35_19605"
     CDS             659..961
                     /locus_tag="VFQ35_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932924.1"
                     /translation="MSGSNATSPSPSVGGDESSEALARVRRGDLSLDEYLETLVEAAV
                     AHLKGRMPEDRLDVIRSIARGTLREAPEFQEAVRSLTGQTPASPSESTPETPSESL"
     gene            965..1645
                     /locus_tag="VFQ35_19610"
     CDS             965..1645
                     /locus_tag="VFQ35_19610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9932925.1"
                     /translation="MIPADSPDVIERFHATLDLVTIVARTLCRRLGGAADFDELVSAG
                     NEGLLDAARRFDPESGTPFRAYARFRVEGAMLDNARRNSGFPRRTRSQLAAEKAALLA
                     RESALTARPKSADSSSANASERSVVNDLAAVVTAAALAFEHAEGAHTEGGDSPEQALS
                     RMQLREAVQSAVDELGPHETELIRRHYFADENLEDVAADLGMSRASASRLHAKALSRL
                     SKLLRRER"
     gene            complement(1694..2041)
                     /locus_tag="VFQ35_19615"
     CDS             complement(1694..2041)
                     /locus_tag="VFQ35_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932926.1"
                     /translation="MTTQGKPQKEELLLNVFCSPLKGSWRVYLGASWLPPYLQGYFDA
                     DGEAYPPELDFEELERQAQKQGAAIEQRIDKLGGVELTARGASAKVLASWLATAFASS
                     AYSPPSQHGKVAG"
     gene            complement(2088..2978)
                     /locus_tag="VFQ35_19620"
     CDS             complement(2088..2978)
                     /locus_tag="VFQ35_19620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932927.1"
                     /translation="MTLPFPPGGSRAADVTQLLAATPYRLVRELGRGVAGRVYEIEHE
                     FLGRRLALKVLHSNNRESTNATRMRVEAQVLGRIQHPNVVEVVDFWVAADGRPCIVME
                     LLEGSTLAARLDEMPQLLAADVVELAAQTLSALVTAHSLGVVHRDLKPENLFLHVPVS
                     GASTVVKVLDFGLVRVLPHAALGTGALPALRTKTGSLVGSPQYMSPEARAGERVDARA
                     DLYSLGVILYVGLTGSGPFDETVGPPAAPSKLRVDGEPSALDALVLRAIAPRIEDRFQ
                     SADEFLEALAPLRAPAPRRR"
     gene            3272..4393
                     /locus_tag="VFQ35_19625"
     CDS             3272..4393
                     /locus_tag="VFQ35_19625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932928.1"
                     /translation="MSAVIAVTDLFEGTPYRLVRALTAGGMAEVYLVAHRGMGRQLVA
                     KVPRAELAENPQIVDRMRVEAQAIGRLKHPNIVSVAGFGTIADGRPYIVTEYLLGRTL
                     TAELAERGRLPLAEALQYSCELLSALIAAHALGIVHRDIKPDNLFVCQKPDGSRSIKV
                     LDFGVARILPDAPEAAPRPLSDATATGMLVGTPRFLSPEAAMGVRVDHRGDIYSAALV
                     IYLLVAGRGPFDHIRGDELLLAAHAVEDPERASNLSEAPVPAELDALLAKALQKDPSE
                     RFQSAEELLKRIESVRTVLLHSPRLLETSVYKPLGDVSAEEASAPAEASGELVVAAEP
                     LPIARPLSFSKGLAVFLLAVVTAAAVGSLLATAIVRSLP"
BASE COUNT          783 a         1488 c         1452 g          790 t
ORIGIN      
        1 tacgaggcga tcgcgcgact caggcgcggg gctccgatgc ggcgcgggcg cgagcgcgcg
       61 gctcagcctt tccacgcttc gccttggatg cgaccaacgc gagccccgct gcggcatcag
      121 tccgccccgc tggggcgtca gttcaccgcg gtgccgttcg aagtcggcga gccagcgttg
      181 ccgacttccc atcccttgat gccgcgttcc aactcccgaa acgcggcgag tacctgcgat
      241 gtcagcgagc tcaaggcggt cacccggtcg tgcacggcga gcacgatggg gacgggcccg
      301 accgacgagc gccttacccg ttctacgtcc gtggcgaggc tctgtgcgat tcggtcgaga
      361 tccgcgagcg tctttagaat ctcatcaacc gcgagacgaa gttcttggag ttcgaccagc
      421 gcttcggagt gcttggtaag taggcattga acgacctctc gcatcgactg tgggtaggca
      481 cacatgagcg ctcctcgcgt cgccggacga atccgctcgg gggcggctcg tctggagcgg
      541 cgcccgcatc gagcaccgcc tacacactgg tcggcgcttc cgcgggaccg atttcgggga
      601 aacgaccgcg ggacgaaaaa aagtgtgagg cttggccgag ccggttagaa tcggctgaat
      661 gtctggttcc aatgccacga gcccgtcacc gagcgttggc ggtgatgagt cgagcgaagc
      721 gctagcacgc gttcgacgcg gagacctgtc gttggacgag tacttggaga cgctggtcga
      781 ggccgccgtc gcccatctga aggggcgcat gccggaagat cggctcgatg tcattcgatc
      841 catcgcgcgc ggcacgctgc gcgaggcgcc cgagtttcaa gaagccgttc gcagcctgac
      901 ggggcagaca cccgcaagtc caagcgagtc gacccccgaa accccaagcg agtcgctctg
      961 agccgtgatc cccgccgact ctcctgacgt catcgagcga tttcatgcga cgctcgacct
     1021 cgtgaccatc gttgcgcgca cgctgtgtcg ccggctcggg ggcgcggctg acttcgacga
     1081 gctggtcagt gcgggcaacg agggcttgct cgatgccgcc cgtcgcttcg atccggagtc
     1141 gggtacgccg tttagagcct acgcccggtt ccgcgtagag ggcgcgatgc tggacaatgc
     1201 ccgccgcaat tcggggttcc cgcgccgcac gcgcagccag ctcgccgcgg agaaagcagc
     1261 gctattggca cgagagtcgg cgctcacggc gcggccgaag agcgccgata gctcttcggc
     1321 gaatgccagt gaacgttcgg tggtaaacga tttggcggcc gtcgtcacgg cggcggcgct
     1381 tgccttcgag cacgcagagg gtgcgcatac cgagggaggc gatagccccg agcaggcact
     1441 gtcccgaatg caattgcggg aggccgtcca gagcgccgtc gacgagctcg ggccgcacga
     1501 aaccgagctc attcgtcgtc actactttgc ggacgagaac ttggaagacg tcgcagcgga
     1561 tctgggcatg agccgagcgt cggcgagccg gctgcacgcg aaagcgctct cgcgcctgtc
     1621 gaagctgctg cgccgcgagc gctgattcga ggccgggaag tgcttgggcg acgtacgcga
     1681 cttcctgtcg ccgctatccg gcgaccttgc cgtgttgcga cggcggcgag tacgcgctgc
     1741 ttgcgaacgc ggtagcaagc cagctcgcga gcaccttcgc cgaagcaccg cgcgccgtga
     1801 gctcgacgcc accaagcttg tcgatgcgct gctcgattgc agcgccttgc ttttgggctt
     1861 gtcgttccag ctcttcgaag tcgagctcgg gcggatacgc ctcgccgtcc gcgtcgaagt
     1921 acccctgcag atacggcggc agccaagaag cgccgaggta cacgcgccaa ctgcccttca
     1981 gcggcgaaca gaagacgttg aggaggagtt cctctttctg gggcttacct tgcgtcgtca
     2041 ctctggcctc cgctctttcc gaatgtacat cgctccacca gggcgagcta gcggcgcctt
     2101 ggcgcaggcg cgcgcagtgg ggccaatgcc tcgaggaatt cgtccgccga ctggaagcga
     2161 tcctcaatgc gcggcgctat cgctcgcaaa accaacgcat cgagcgctga aggctctccg
     2221 tctacgcgga gtttcgacgg tgcggcaggc ggccctactg tttcgtcgaa gggcccgctc
     2281 ccggtgaggc ccacgtaaag gatcacgccc agcgaataga gatcggcacg cgcgtccacc
     2341 cgctcgcccg cgcgtgcttc gggactcatg tattggggcg accccacaag cgagcccgtc
     2401 ttcgtgcgca acgcaggcag tgcgccggta ccgagcgcgg catggggcag aactcgcacc
     2461 agtccgaagt cgagcacctt gacgacggtc gacgctccac tcacagggac atgaagaaac
     2521 aagttctcgg gcttcaggtc gcggtgaacg acgccaaggg agtgagccgt cacgagcgcc
     2581 gagagggttt gcgcggcgag ctcgaccacg tccgcggcaa gcaactgcgg catctcatcg
     2641 agccgtgccg ccaaggtgct cccctccagc agctccatca cgatgcaggg gcgcccgtct
     2701 gcggcaaccc agaagtcgac cacttcgacc acgttggggt gctgaatgcg ccccaggact
     2761 tgggcctcga cgcgcatcct tgttgcgttc gtcgactctc ggttattcga gtgcagcacc
     2821 ttcaacgcca ggcgccgccc gaggaactcg tgctcgatct cgtagacccg acccgcgaca
     2881 ccccgtccca gctcgcgaac caagcgatac ggggtcgctg cgagcagctg ggtcacgtcg
     2941 gcagctcggg agccgcccgg tgggaacggg agcgtcatgt acacctatcg gcgccaaccg
     3001 aagcgcaatt ccagagaatc gacatgggga tggctttttg ctccgctgtc ctcgtcgaag
     3061 ctattgggcg acagaaaagt gaacgcgccc ctacgaattt gcggaaacgg accgatgttc
     3121 ggcacgacaa acaagtgttg gcccacatcg ttccgcttcg ccagagtttg ggcccgggca
     3181 attcctgtgt gattcaaaga acttgcatcc actacggagt ttgcgcctgg gagcggtcgg
     3241 gcccccttcg tatgctcatc tgggcttgcc gatgtccgcc gtaatcgccg tcaccgacct
     3301 gttcgagggc acgccctacc gtctcgtgcg cgctctcacg gctggcggaa tggccgaagt
     3361 ctacctggtc gcgcaccgcg gcatgggacg gcagctcgtg gcgaaggtgc cccgcgcaga
     3421 gctcgccgaa aaccctcaga tcgtcgatcg catgcgggtc gaagcgcaag ccatcgggag
     3481 gctcaagcac cccaacatcg tttccgttgc gggcttcggc accatcgcag acggccgccc
     3541 ctacatcgtt accgagtacc tgctcggtcg cacgctgact gccgagctcg ccgaacgcgg
     3601 acgcctgccg ctcgccgagg cactgcaata tagctgcgag ctcctgtccg cactcatcgc
     3661 ggctcatgcg cttggcatcg ttcaccgaga catcaagccg gacaacctct tcgtctgtca
     3721 gaagccggac ggctcccgct ctatcaaggt gctcgacttc ggcgttgcgc ggattctgcc
     3781 cgacgctccg gaagccgcgc cgcgcccgct ctcggacgcg accgccacgg gcatgttggt
     3841 tggcactccg cgcttcctca gccccgaagc cgcgatgggt gtgcgcgtcg accatcgcgg
     3901 cgacatctac tccgccgcgc tcgtcatcta tctgctcgtc gcggggcgcg ggccgttcga
     3961 ccacattcgc ggcgacgaac tattactcgc agcccacgcc gtcgaagatc ccgagcgtgc
     4021 gtcgaatttg agcgaagcac cggtgccagc ggagctcgac gcgcttctcg ccaaggcgct
     4081 tcaaaaggac ccgagcgagc ggtttcagag cgcggaagag cttctgaagc gcatcgaatc
     4141 ggtccggacc gtgctccttc actcgccacg cttgctcgag acgagcgtgt acaagccgct
     4201 gggcgacgtc agtgccgagg aagcttcagc tcccgcggag gcctctggtg agttggttgt
     4261 cgcagctgaa ccgttgccca tcgctcgacc gctgtcgttt tcgaaagggc tcgcggtatt
     4321 tctccttgcc gttgtcacgg ccgccgcggt cggctctctg ctggctactg cgatagtcag
     4381 gagtctgccg tgactcgaaa cggggaggtt caacgcagca tgactccgcg cacggagaac
     4441 gagctcgacg ttggcccggt gaagcccggg gagcttttcg agggctacca gatcgtcgag
     4501 ctccttggtc agg
//
LOCUS       DASTKS010000936         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1937243, whole genome shotgun sequence.
ACCESSION   DASTKS010000936 DASTKS010000000
VERSION     DASTKS010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937243"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFQ35_19630"
     CDS             complement(<1..929)
                     /locus_tag="VFQ35_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9932929.1"
                     /translation="MIFDWLYGLFSNDLAIDLGTATTLIYVKGKGIVSCEPSVVAVSR
                     DARGEKRVLAVGREAKDMLGRTPGNIQAIRPLRDGVIADFEITEAMLRYFIMRAQTRR
                     TLVKPRIIICVPFGITEVETRAVKESAESAGAREVYLIEEPMAAAIGAGLPITEPSGN
                     MVVDIGGGTTEVAVISLAGIVYSQSVRVGGDKMDEAIVAYMKRKYNMAIGEQTAERIK
                     ITIGNAYPLEQQLTMEVKGRDMVAGIPKTVVVNSDEIRDSLSEPTNAIVEAVLIALER
                     TPPELAADIVDKGIVLTGGGSLLKNLDVLLREET"
     gene            complement(1369..2244)
                     /locus_tag="VFQ35_19635"
     CDS             complement(1369..2244)
                     /locus_tag="VFQ35_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932930.1"
                     /translation="MATEDARKLFVAGLPESITEDVLRQLFEATGGKVVEVSLPRERT
                     TGKVRGFGFVTFATADEASRARDELDGSLQAGRSISVRPFSSEPPKRGERTEGGGGGS
                     GRFEAAAAQGPTEDRTLYVGNLPYDTNQQELSQFLEENGCGPVARIHLPVGPDGRMRG
                     FGFVTMASAEAANGALAALRNAEVRGRRLMINIAHPRSDRPPAGPGGDRSRPPPRDAG
                     PRFPTEPPMNFESGGAAPERRRTSMPAEGEGTEEARRNKKKKAKGASTGRATEQRERG
                     GGGSWKNRVDWDDDD"
     gene            2425..>2986
                     /locus_tag="VFQ35_19640"
     CDS             2425..>2986
                     /locus_tag="VFQ35_19640"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9932931.1"
                     /translation="MTLRPSMRVRVIVNPKAGSGTAAKRLPLILREIERAGALPDSAE
                     TLGPGDAARLVREAHRDGVDCVALVGGDGTLNEAVQAFVGPGGELLPGPVLGLIPSGT
                     GGDFRKTFNLGTSTEEAVARLMSSPPRPIDLGLLQLTSHEGLPIHRAFINVTSFGMGG
                     LTDRIVNSSPKWWGGRASFFIGALRAL"
BASE COUNT          478 a         1032 c          910 g          566 t
ORIGIN      
        1 gtctcttcgc gcaggagcac gtcgaggttc ttcaggagcg agccgccgcc ggtcagcacg
       61 atgcccttgt ccacgatgtc ggccgcgagc tcgggcggcg tgcgctcgag cgcgatcagc
      121 accgcctcga cgatcgcgtt cgtgggctcg ctgagcgagt cgcggatctc gtccgagttg
      181 accaccacgg tcttggggat gccggcgacc atgtcgcgcc ccttgacctc catcgtgagc
      241 tgctgctcga gcgggtacgc gttgccgatg gtgatcttga tgcgctcggc cgtctgctcg
      301 ccgatcgcca tgttgtactt gcgcttcatg taggcgacga tcgcctcgtc catcttgtcg
      361 ccgccgacgc gcacgctctg cgaatagacg atgcccgcga gcgagatcac ggccacctcg
      421 gtggttccgc cgccgatgtc gacgaccatg ttgccgctcg gctcggtgat gggcagcccc
      481 gctccgatcg ccgccgccat gggctcctcg atcagataga cttcgcgagc gccggcgctt
      541 tcggcgctct ctttcaccgc gcgcgtctcc acctcggtga tgccgaaggg cacgcagatg
      601 atgatgcgcg gcttcacgag cgtgcggcgc gtttgcgcgc gcatgatgaa gtagcgcaac
      661 atcgcctcgg tgatctcgaa gtcggcgatc acgccgtcac gcagcgggcg aattgcctgg
      721 atattgccgg gtgtacgccc caacatgtcc ttggcctcgc ggccgacggc gagcacacgt
      781 ttttcaccgc gcgcgtcgcg tgagaccgcg accaccgaag gctcacagct aacgatgccc
      841 ttccctttga cgtaaatgag cgtcgttgct gttccgagat cgatggccag gtcgttcgag
      901 aacaggccgt aaagccaatc gaaaatcatc gaccgtgctc aggggagaaa cgagggagca
      961 agttcacgct gcgtttgctg acggctgcct aaggcgagcc aattggcgaa gggggccacg
     1021 tcctaccacg cactctctcg cgcgcgcaat cgctgcctaa ccgaccaggc ctaacagccg
     1081 acccgtcact tcggagaatt cacagccggg cgattaaacc tgacgcaaag cctgaagttt
     1141 tctcgcttcg cgccaaggca tcacagcgag ttctgcgcct gcaggctcgt tggccaagag
     1201 cgcaagctgc tgcccgccga gccattcccg cggcgccttc gcctcacgcg ttcgacatac
     1261 ccttcgcagt tatgttccag ggtgagacct cgcgcgcggc gcgcctccgc cgaatgcact
     1321 cgctcgacgc gccgctgctg agcgcctttc gctagggccg cgagttcttc aatcgtcgtc
     1381 gtcccaatcg acgcggttct tccagctgcc gccaccgccg cgctcgcgct gctcagtcgc
     1441 gcgacccgtc gaagcgccct tcgccttctt tttcttgttg cgacgagctt cctcggtgcc
     1501 ctcgccttcg gcgggcatgc tggtgcgacg ccgctcgggc gctgcaccgc cgctctcgaa
     1561 gttcatcggc ggctcggtgg ggaaacgcgg acccgcatcg cgcggcggag ggcgcgaacg
     1621 atcaccgccg ggcccagccg gaggacggtc gctccgcggg tgcgcgatgt tgatcatcaa
     1681 ccgccgcccc cgcacctctg catttcgcaa cgcagccaac gcgccgttcg cggcttctgc
     1741 gctcgccatc gtcacgaacc caaagccgcg catacgaccg tcgggcccca ccggcaagtg
     1801 gatgcgagcg actggcccgc aaccgttctc ttccaagaac tgagacagct cttgttggtt
     1861 ggtgtcgtac ggcagattgc caacgtagag cgtgcgatcc tccgtcggtc cctgcgctgc
     1921 agccgcctcg aagcggccgc tcccgccgcc accgccctcg gtgcgttcgc cgcgcttcgg
     1981 tggctccgaa gagaacggac gcaccgaaat ggatcggcct gcttgcaacg aaccgtctag
     2041 ttcgtctcgc gcacgactcg cttcgtccgc cgtcgcaaac gtcacgaatc caaatccacg
     2101 aaccttgcct gtcgtccgct ctcttggcag gcttacctcg acgacctttc caccggtcgc
     2161 ctcgaacaac tgccgcaaga catcttccgt gatggactcc ggcaagcccg caacgaagag
     2221 cttgcgcgca tcctcggtcg ccattctcga ttaccttgtc gccctgaaaa acagttccta
     2281 ctcgccgggc cggatcgacc cgcgcaggga accagacttt tacgataact gtcgccgttt
     2341 cgcggttcaa gccctacgat cgaaccggcc gagcgcccga cgactcccaa ggcggctcga
     2401 gggttgccga gcggcttccg atttgtgacc ctgcgaccct ccatgcgcgt ccgcgtcatc
     2461 gtcaatccca aggctggcag cggcactgct gccaagcggc ttccccttat cttgcgcgag
     2521 atcgagcggg ccggagcact acccgacagc gccgaaacgc tggggcctgg cgacgcagcg
     2581 cgtctcgttc gcgaggctca tcgtgacgga gtcgattgtg tcgcgctggt tggcggcgac
     2641 ggcaccttga acgaggcggt ccaggctttc gtcggtccgg gaggggagct tcttcctggt
     2701 cccgtgctgg gcctcatccc gtcgggcacg ggcggcgact ttcgcaagac gttcaacctc
     2761 gggacctcta ccgaggaggc agtggcgagg ttgatgtcga gcccaccacg accgatcgat
     2821 ctcgggctcc ttcagctgac gtcgcacgag gggctgccca ttcaccgcgc gttcatcaac
     2881 gtgacgagct ttgggatggg tgggctcacc gatcgtatcg tgaactcgag cccgaaatgg
     2941 tggggtggac gcgcgagctt cttcatcggc gcgctgcgag ctcttg
//
LOCUS       DASTKS010000937         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1937344, whole genome shotgun sequence.
ACCESSION   DASTKS010000937 DASTKS010000000
VERSION     DASTKS010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937344"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VFQ35_19645"
     CDS             <1..1306
                     /locus_tag="VFQ35_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932932.1"
                     /translation="VGGSGAGGAESVPARTREGDANGGVGGVGGEGGDGGRVQGPSAG
                     ANEPVSCASLAEAKKVLYVTGSSAARPFLELIARQLTLQGVYLVYSATGSCVGVDAAL
                     NGTALSATATYWDSVVSTGATCRLSANGVTTDVAVSDVFAETCPGFELANLSALGVAD
                     AHGPIQTMAFVVPASSPQRVISSQAAYFVYGFGQAGGVLDLDGVRRVWSDEGLVFQRN
                     ASSGTQALLAAAIGVPAERFRGQSVKSSDDMAASIQAAGIDPERAANTIGLLAADFIE
                     SRDLRASVRVLAYQDSRQFCGFYPDSTATARDKQNVRDGHYALWGPLHLLYKVDSLGN
                     PRNPENRELVLAMVGYLSGSKPLPNGVRLIDVYAQSGLVPECAMRVQRTKDGGNVTPF
                     RPNNPCGCLFEVSATGATACKPCTVQGDCSDGETCSLGYCEP"
     gene            1382..>2985
                     /locus_tag="VFQ35_19650"
     CDS             1382..>2985
                     /locus_tag="VFQ35_19650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932933.1"
                     /translation="MILDARTDAKQGSSNELARATDPAFARYRSLGMIARGGMSEVYL
                     VSWDGDAGVERLAVMKRLSAELANDPEAVQMFHDEGRIGLRLNHPNLVQTYDVGRYEG
                     RPCILFEHLRGQPLNRVIQRAIERRIPIPLNVAVQIMVGVLDGLAAAHEACDYDGTLL
                     DLVHRDVSPHNVFIEYDGRIKLLDFGVARATTHEAVTRTGLVKGKFAYMAPEQARGES
                     VDARADVWSAGVVFWELLTGTRLFRGANEAAVLQATLNAPIASAQTLRPNLPLELEQI
                     LQRMLRRDPAERYEDATTARRDLERWLGAQTSSATRFLRTHMAVAFGEEQEREQKQLA
                     EILSSQPPRHAPPRSGRMVVGTHGGTVNVDRTPTIARPLAEDERTQLLNALERKSRVA
                     LIAVVAAVCLFGVLLGAGLMSTRSSYGSVAPHGTAATTTVQPSEPITAPSALTASLVA
                     SDPRRGAVVTEAPSGAEVTALARPNGRVPDRSRVPDVGRRAVGGAIRAPVSGTTADET
                     RVGYLTLDSTPWSTVSVGSRVLGETP"
BASE COUNT          525 a          913 c         1015 g          532 t
ORIGIN      
        1 agtcggtggc agcggagcgg gtggagctga gagcgtgccg gcgcgcaccc gggagggcga
       61 tgcgaacgga ggagttggag gagttggagg cgagggcggt gatgggggac gcgttcaggg
      121 gccgagcgct ggcgcgaacg agccggtttc gtgcgcttct ttggccgagg cgaagaaggt
      181 tttgtacgtg accggctcct ccgcggcgcg gccatttctc gagctcatcg cgcgtcagct
      241 cacgctccag ggcgtgtatc tggtctattc ggcgacgggc tcgtgtgtgg gagttgacgc
      301 ggcgctgaat ggaacggcgc tgagcgctac cgcaacttac tgggacagcg tcgtcagcac
      361 gggcgctacg tgtcggctct ccgcgaacgg tgtcaccact gacgtcgcgg tgtcggatgt
      421 atttgccgaa acgtgtcctg gcttcgaact cgccaatctg agcgcgctcg gtgtcgcgga
      481 cgcgcacggg ccgatccaaa cgatggcgtt cgtcgtgccc gcgagttcac cgcaacgagt
      541 gataagctct caagcggcgt acttcgtgta cggcttcggg caggccggcg gcgtgctcga
      601 cctcgacggc gtgcgtcgtg tgtggtcgga cgagggtctc gtgtttcagc ggaatgcgag
      661 ttcgggaaca caggctctgc tcgcggccgc catcggtgtg ccggcagaac gcttccgcgg
      721 gcagtcggtc aagagctcgg acgacatggc ggcgagcatt caagccgccg gcatcgatcc
      781 agagcgcgcc gccaacacga ttggtttgtt ggcggccgac ttcatcgaga gtcgcgactt
      841 gcgcgcttcg gtccgcgtgc ttgcgtatca agatagtcgt caattttgcg gcttctaccc
      901 tgactcgacc gcaacggcgc gcgacaagca gaacgtgcgg gacgggcact acgcgttgtg
      961 ggggccgctg cacctcttgt acaaggtgga cagcctcggg aacccgcgga acccggagaa
     1021 ccgtgagctc gtgctcgcaa tggttgggta cttgagtggt tcgaagccgc tgcccaatgg
     1081 agtcaggttg attgacgtgt acgcgcagag cgggttggtt cccgagtgcg cgatgcgcgt
     1141 gcagcgaacg aaagacggcg gcaatgtgac cccgtttcgc cccaacaatc cctgcgggtg
     1201 cctgttcgag gtcagcgcga cgggcgcaac cgcttgcaag ccttgcaccg tacagggcga
     1261 ttgttcagat ggcgaaacct gttcgctcgg ctactgcgaa ccttgagctc atccacatgt
     1321 ttcaccaacc aactgctgag ttcaccaact cgccccagtg acccaataca tttctcgatc
     1381 catgatcctc gacgcaagaa ccgacgcgaa gcagggtagc tcgaacgagc tggcgagggc
     1441 gacggatccc gcattcgctc gctatcgttc gttgggcatg atcgcccgcg gcggcatgtc
     1501 cgaggtgtac ctcgtcagct gggacgggga cgcgggggtc gagcggctgg cggtcatgaa
     1561 gcggctgagc gccgagctcg cgaacgaccc cgaagccgtt cagatgttcc acgacgaggg
     1621 ccgcatcggg ctccggctga accacccgaa cctcgtgcaa acatacgacg ttggccgcta
     1681 cgaggggcgc ccgtgcatct tgttcgagca cttacgcggg cagcctttga atcgagtgat
     1741 ccagcgcgcc atcgagcgcc ggatcccaat cccactgaac gtagcggtgc aaatcatggt
     1801 cggcgttctc gatgggctcg cggccgcaca cgaagcctgt gattatgacg gcacgctgct
     1861 cgatctggtg caccgtgatg tgtcccccca caacgtgttc atcgaatacg atggacgtat
     1921 caagttgttg gacttcggcg tcgcgcgggc aacgacgcac gaagcggtga cccgcaccgg
     1981 tctcgtcaaa ggaaaattcg cgtacatggc gccggagcaa gcgcgcggcg agtcggtgga
     2041 tgcacgcgcg gacgtgtgga gcgcgggggt cgtcttttgg gagctcctca cgggcacgcg
     2101 actgttcaga ggggcgaacg aagccgcggt gctgcaagca acgctcaacg cgcccatcgc
     2161 ctcggctcag actttacgtc ccaacctgcc tctggagctc gagcagatct tgcagcggat
     2221 gttgcgccgc gatcccgccg agcgctacga agatgcgact acggcgcggc gcgacctcga
     2281 gcgctggctc ggcgctcaaa cctccagcgc aacgcggttc ttgcgcacgc acatggcggt
     2341 ggcttttggc gaagaacaag agcgcgagca gaagcagctt gccgagatct tgagctcgca
     2401 gccgccccgt cacgcgccgc cccgctcggg tcggatggtt gtcggcacgc acggtggcac
     2461 cgtgaacgtc gacaggacgc cgacgatcgc gaggcccctt gccgaagacg agcgcacgca
     2521 gctgctgaac gctctcgagc gaaagagccg cgtcgcgctc atcgcggtcg tcgcagcggt
     2581 ttgccttttc ggggtgttgc tcggcgcagg cctcatgtcg acgcggtcga gttacggcag
     2641 cgtcgcgccg cacggtacgg cggcgacgac gaccgttcag ccctccgaac caataaccgc
     2701 tccgtctgca ctcacggcgt cgcttgtcgc gagtgaccct cgacgaggtg cggtggttac
     2761 cgaggcgccg tctggcgctg aagtgaccgc gcttgcacga cccaatggga gagttcccga
     2821 ccgcagccgc gtgccagatg tcggccgccg agctgttgga ggagcaattc gcgcgccggt
     2881 atcgggaacg accgccgatg aaacacgagt gggttacttg accctcgaca gcacaccttg
     2941 gtccacggtt tcggtgggga gccgcgtcct cggagaaacg ccgat
//
LOCUS       DASTKS010000938         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_595839, whole genome shotgun sequence.
ACCESSION   DASTKS010000938 DASTKS010000000
VERSION     DASTKS010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595839"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="ccsA"
                     /locus_tag="VFQ35_19655"
     CDS             <1..408
                     /gene="ccsA"
                     /locus_tag="VFQ35_19655"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9932934.1"
                     /translation="AANVLGVGLFLLAGAAGAFYVVEERRLKHKKLGKASRLPPLASL
                     DRAAHRLLLAGFPLLTFGVVTGSMFLDRLTTVHGLELLRSLVGYASWLSFAIVLVLRH
                     SSGVGARRAAYGTLVGVAGVLVVMLAYVFGASQ"
     gene            455..1852
                     /gene="hemA"
                     /locus_tag="VFQ35_19660"
     CDS             455..1852
                     /gene="hemA"
                     /locus_tag="VFQ35_19660"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9932935.1"
                     /translation="MIVVVGLSHRTAPIEVRERIALNREKLPEFLSTLASEPPVGEAF
                     VVSTCNRVEIVAAARAGEDLQAVADACVKALEREAPGIGAHLYRRVGGDAVRHLFRVA
                     SSLDSLVLGEPQILGQVKDGFDLGRALGTIGSTLHRSVPRALRTAKRVRSETAIGSGQ
                     VSVPSVAVDLTRQIFGELRGRGVLLVGSGEMAEAVARLLRGAGSRLFVLGRNRERVEQ
                     LAREVDGEPRLWPDLTATLVEADVIITSTSAPGFVIDREQVLAARKKRRGREQSYIDL
                     AVPRDVEPSVNEIEGVFLYNIDDFSQIVADSLAQRSREAEQAARIVDEETRDYERWAD
                     AEQATPTIVRLRQKLRAALRVELERSFRGRLKHLPAADRQALELMLDAAENRLLHGPT
                     MRLRLAAAEPDSSELSLAELTIALDELFELNTDVRDSSPAASEGAALASRPRATTEAE
                     PERPQEHNSRWPNSQ"
     gene            1834..>2623
                     /gene="hemC"
                     /locus_tag="VFQ35_19665"
     CDS             1834..>2623
                     /gene="hemC"
                     /locus_tag="VFQ35_19665"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9932936.1"
                     /translation="MAEFTVTLATRKSALALAQSRAFARTLEQANPGLTVKELRVTTT
                     GDRIQDRTLNEIGGKGLFVKEIEEALLDGGADLAVHSLKDVPPDLPQGLCIGCYPERE
                     DPRDVLVSRDGRKLLDLAHGSRIGTSSLRRRLQLELARPDLRVEVLRGNVDTRLRKCR
                     EGVVDAIVLARAGMVRLGIADQATEVIDPSVMLPAVGQGCLGIEHRSDDARVAALLAP
                     LAHADTRIAVLAERAVMRAVEGSCQMPVAAYAVRDGDRLLLRGLL"
BASE COUNT          393 a          879 c          898 g          453 t
ORIGIN      
        1 gccgcgaacg tgctcggcgt cgggctcttc ttgttggcgg gcgcggcggg cgcattctac
       61 gtcgtcgaag agcgccgctt gaagcacaag aagctcggca aggcttcgcg tttaccgccg
      121 ctcgcgtcgc tcgatcgagc ggcgcatcga ctactgctcg cgggttttcc gttgctcacg
      181 ttcggcgtcg tcacaggttc catgttcctg gaccgcctca cgaccgtgca cggcctcgag
      241 ctcttgcgct ctctcgtggg ttacgcgagc tggctctcgt tcgcgatcgt gctcgtgtta
      301 cgacactctt ctggcgtcgg tgctcggcgg gcggcctacg ggacgttggt gggcgtcgcg
      361 ggggtgctgg tcgtgatgct cgcctacgtc ttcggcgcga gtcagtgatg acacggggcc
      421 cctgcttcca ttcgtgttgg ccgcggagtg gcgcgtgatc gtagtcgttg gtctctcgca
      481 ccgcaccgcg ccgatcgaag tgcgcgagcg catcgcgctc aaccgcgaaa agctcccgga
      541 gtttctctcg acgctcgcga gtgagccgcc ggtgggcgaa gcgttcgtgg tttctacgtg
      601 caaccgcgtg gagatcgtcg cggccgcgcg cgcgggcgaa gatcttcagg cggtggcgga
      661 cgcctgcgtg aaagcgctcg agcgcgaagc gcccggcatc ggagcgcacc tctaccggcg
      721 cgtgggcggc gacgcggtga ggcacctgtt ccgagtcgcc tcatcgctcg actcgctcgt
      781 gctcggtgag ccgcagatct tgggccaggt gaaggacggc ttcgacctcg gtcgtgcgtt
      841 gggtaccatc ggttcgacgc tgcatcgctc ggtgccgcgc gcgctccgca ccgccaagcg
      901 cgttcgaagc gagactgcaa tcggctctgg gcaggtctcg gtgccgagcg tcgcggtcga
      961 tctcactcgg caaattttcg gggagctccg cgggcgcggc gtgctcctcg taggctcggg
     1021 agaaatggcg gaggcggtcg cgcggctgtt gcgcggcgcc ggctcgcggt tgttcgtgct
     1081 cggccgcaac cgtgagcgcg tcgagcagct cgctcgggag gtcgatggcg agcctcgcct
     1141 gtggcccgac ctcacggcga cgttggtcga ggccgacgtc atcatcacga gtacgagtgc
     1201 tcccgggttc gtcatcgatc gcgagcaggt gctcgcggcc cgaaagaagc gccgcggccg
     1261 cgaacagagc tacatcgatc tcgccgttcc acgtgacgtc gagccgagcg tcaacgaaat
     1321 cgaaggcgtg ttcctctaca acatcgacga cttttcgcag atcgtcgccg actcgctggc
     1381 gcagcgcagc cgggaggcgg agcaagccgc gcgcatcgtc gacgaagaga cgcgagacta
     1441 cgagcgatgg gccgacgcgg agcaagccac gcccacgatc gtccgcctcc gccaaaagct
     1501 gcgcgccgcg ctgcgggtcg agctcgagcg gagctttcgg ggccgcttga agcacctgcc
     1561 cgcggcggat cgccaggcgc tcgagctgat gctggacgcc gccgaaaatc gcttgctgca
     1621 cgggcccacg atgcggctac ggctcgccgc ggccgagccc gactcctccg agctctcgct
     1681 cgcggagctc accatcgctc tcgacgagct cttcgagctg aacaccgacg tccgcgattc
     1741 ttctccggct gcgagcgagg gtgccgcgct cgcgtcgcgt ccgcgtgcca ccaccgaagc
     1801 cgaacccgag cgcccccaag agcacaactc acgatggccg aattcacagt aactctcgcg
     1861 acacgcaaaa gcgcgctcgc cttggctcaa tcgcgcgcgt tcgcgcgaac cctcgagcag
     1921 gcgaacccag gtctcaccgt gaaggagttg cgggtcacca cgaccggtga tcgcatccaa
     1981 gaccgcacct tgaacgagat tggcggcaag ggactcttcg tcaaagaaat cgaggaagcc
     2041 ctgctcgacg ggggcgccga cttggcggtg cactcgctca aagacgtgcc gcccgatctc
     2101 ccgcaaggtt tgtgcatcgg ttgctacccc gagcgcgaag atccgcggga cgtgctcgta
     2161 tcgcgcgatg gtcgcaagct cctcgacctc gcgcacggca gccgcatcgg gacgtcttcg
     2221 ctgcgccgtc gcttgcagct agagctcgct cggcccgatc tgcgcgtcga ggtactgcgc
     2281 ggcaacgtgg acactcggct tcgcaagtgt cgagagggcg tcgtcgacgc catcgtgctc
     2341 gcgcgtgccg gcatggttcg cctcggcatc gccgaccagg cgacggaggt catcgatccg
     2401 agcgtcatgc tgcccgcggt aggacaaggc tgcctcggca tcgagcaccg cagcgacgac
     2461 gcgcgcgtcg cggctttgct cgcgccgctt gcgcacgcgg atacgaggat cgcggtgttg
     2521 gccgagcgcg ccgtaatgcg cgcggtcgag ggaagctgcc aaatgccggt tgcggcctat
     2581 gcggtgcgcg acggcgaccg actgctcctc cgcgggctgc tcg
//
LOCUS       DASTKS010000939         8842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_595920, whole genome shotgun sequence.
ACCESSION   DASTKS010000939 DASTKS010000000
VERSION     DASTKS010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595920"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFQ35_19670"
     CDS             complement(<1..452)
                     /locus_tag="VFQ35_19670"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET9932937.1"
                     /translation="MQRSCVGCRSPVDPGELVRLVLGPEGEVVVDLARGAFGRGAWVH
                     PRPECIEKAAPQGLSRAFKCQVRDNGTQVLATFRAAAERRVQGLVLAARRAHKLEAGA
                     SAVEEAVRARRAALVLVATDARASADLAWLAPLVANGQALAFSTKATFA"
     gene            complement(458..2158)
                     /gene="nusA"
                     /locus_tag="VFQ35_19675"
     CDS             complement(458..2158)
                     /gene="nusA"
                     /locus_tag="VFQ35_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9932938.1"
                     /translation="MAQAAGYTEGLGSIVEQVAQEKGIDKKILIETMEAAILKAAQAA
                     FGPNRELEARYNSDTGNIDLFQYMTVVEAVAQPEREIAVDQARKHGLDADIGEELGFQ
                     VFWHPRDAERARQQDKEFGSVLDMKQARSQFGRIAAQTAKQVLLQRVRDAERDIIHNE
                     YKDRQGQLIRGIVRRFEKGHNLIVDLGKTEGVLPAREQTPRETYRPGDRIVAYVKNID
                     RESRGPMIILSRQSPHLVEKLFEAEVPEIYEGIVKIVSVAREPSSRSKIAVTSRDPDV
                     DPVGACVGIKGSRVQAVVQELRGEKIDIVPYDKDPARYIINAIQPAEVNRVVVDEANH
                     RMELVVPDEKLSLAIGRKGQNVRLASQLTGWRLDIVSESKFRQMEDEAMIQLAEIEGM
                     DEELAKNLFGLGFRALDEIAEAELAELATIEGVEDEARASELQQAAERALERLRQERI
                     RRIAEQSGALSDHDALLVVPGMGTRTAQALEEAGYKSVRDVAKEDPDRLAIKSGLGNK
                     KARQVWQGAVDFQRTVAKEIQTAREHLADQRAELNAQAEADVAASVGADEEEVSEEES
                     "
     gene            complement(2168..2728)
                     /gene="rimP"
                     /locus_tag="VFQ35_19680"
     CDS             complement(2168..2728)
                     /gene="rimP"
                     /locus_tag="VFQ35_19680"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET9932939.1"
                     /translation="MSGLDRKRVLDAVLPVLNAHAVDAVELVWRTDRSGWVLELTVER
                     PDSRVPGEGVTIDLCSEISRDLSAALDVADAIPHRYRLEVGSPGLERALYTARDYARF
                     AGQFARLKLKAPIEGQHVLRGTLHGLDEQGAVLLESERGELVPLSLDGIESARLVFQS
                     GAGARGGKGSPGRASGRGARAHRPQR"
     gene            3096..3464
                     /locus_tag="VFQ35_19685"
     CDS             3096..3464
                     /locus_tag="VFQ35_19685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9932940.1"
                     /translation="MSKRILVVDDSKAMRLIVRRTLRQAGFGDYEVEEACNGLEAISA
                     IENARPDLILSDWNMPEMNGYELLKALRAKKNDVRLGFVTTESTPEMQAMANEAGALF
                     LIAKPFTAEQFQAALKPFLG"
     gene            complement(3471..4682)
                     /locus_tag="VFQ35_19690"
     CDS             complement(3471..4682)
                     /locus_tag="VFQ35_19690"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET9932941.1"
                     /translation="MRVLVADRLTEDSLDEMRSLGVELTYDPELRPEKLPEALEGVNV
                     LIVRGTEVSRAALRAGRALNLIIRAGAGVSNIDVVAASERGIYVANCPGKNAAAVAEL
                     TFTLIGSLDRRVPDAVMSLRAGRWEKDEYARAVGLSGKRIGILGLGHVGRAVLRLAQA
                     YNMKCYAFGRSLTGAKAQELGVQIARSPEELAGESDIFTVHLELAERTRGIVDRRVIE
                     ALPDDAMFINTARPELVDYDALAELIPKKRLRVGLDVLPKEPGSRSGTYEHPILKSEF
                     VYATPHIGASTDQAQTAIAAECARILRCFITKGEVPNVVNISAAFWSRYQLVVRHIDR
                     VGALANVLAVLKRHGIDIHELDNTVFEGGRAACAKIRLGSRPSEDCLSEIMAFSDEIL
                     HVDLVTLPNFA"
     gene            complement(4755..5273)
                     /locus_tag="VFQ35_19695"
     CDS             complement(4755..5273)
                     /locus_tag="VFQ35_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932942.1"
                     /translation="MKPSDHPEFFRLPPPEGRSRESTIVLDSEGRFFHDGAPVTHPGM
                     ARAFSSWIARHPDDGRFILNNGYDWSYFRVEDAPFQVISLILPDDSRASGCLQVELSD
                     GTLEDLGPESLEIGPNGAVYAAVKGGAFQARFSRAAQLALAPLLAEDGDGALALDFAG
                     QRVRLPISSRKA"
     gene            complement(5270..6475)
                     /locus_tag="VFQ35_19700"
     CDS             complement(5270..6475)
                     /locus_tag="VFQ35_19700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HET9932943.1"
                     /translation="MSLKVLILSRIAGSYSTQRLKEACVRRGHSARVLNTLNFSLLVE
                     RAQPRLYYRDRVLSRYDAAIPRIGASVSSFGTAVVRQLEQMGVFTLNSAQAIGTSRDK
                     LRAFQILSAHEIAFPPTAFVRDRESARSALSQVGGAPVIIKLTEGSQGAGVILADTKK
                     TAEAIVEAFLVARQDVIVQKFVSESRGHDLRALVVGDRVVAAMRRRAAPDEFRSNVHR
                     GGRTERARLSPELEALALKSAQILGLRVAGVDLIESDDGPLVLEVNSSPGLEGIESAT
                     GIDVAGEIVQHMEEQIQFPEIDLRQRLGAAPTHGTAELVVGPGSGLEQRTLAALELSS
                     RKVLVLTVTRGARVVPTPGLDFQLLRGDRLLCFGPSDALRALMSTAASEGAQSETPSD
                     ESWPLRARQ"
     gene            complement(6499..6990)
                     /locus_tag="VFQ35_19705"
     CDS             complement(6499..6990)
                     /locus_tag="VFQ35_19705"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET9932944.1"
                     /translation="MRSARAERLVIGLTEYVDIAEWRVRGLRAKVDTGARSSALHVEN
                     LREIGRDRVRFDVRLHRSKVDRRVSVEAPIVRRARVKASTGVSATRVFVEATIQIGPF
                     TERIELGLVDRANMLYRMLLGRTALGSHFFVDPARRYLLTGHRRAVSPGKPKPAPARR
                     SRH"
     gene            7176..7859
                     /locus_tag="VFQ35_19710"
     CDS             7176..7859
                     /locus_tag="VFQ35_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932945.1"
                     /translation="MTDEFPTLEVVSAVCVSWLAFRDWVLLRGASRLNDDIARVARRL
                     EQNEAGNERGAAPWLRPVTRTVNALLAGEIGTTTARELLIERSLRARRKLTSGAARDL
                     VVCAVLAGALIYARAAKLEVGSPFFGLGIAALVMLLAGAALRVRLERVLGRAGARVAE
                     AIARRPSAAASGAAPRCRICREPKLSRVASPEGLGAQLSALGVVEVFVCPRCGHVTGA
                     AHPPSQTKV"
     gene            7909..8649
                     /locus_tag="VFQ35_19715"
     CDS             7909..8649
                     /locus_tag="VFQ35_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9932946.1"
                     /translation="MDASGSHDSNRRYYDAFSARYEAQRGENDPGGYHELIDSLETAF
                     VERYGRGREVLEVGCGTGLLLRRIRDFASTARGVDLSPGMLALAKERGLDVVEGSATA
                     LPFADQSFDVTCSFKVLAHVREIETALAEMARVTRPGGHVIAEFYNPMSLRGLVKRFG
                     PAGSVAHDTHEKQVFTRFDSPRRVRALTPAGCRQVAARGVRIATPTAHLMKTRLGREV
                     FRRAEELLCDSPLKVFGGFYVVAFEKQS"
     gene            8653..>8842
                     /locus_tag="VFQ35_19720"
     CDS             8653..>8842
                     /locus_tag="VFQ35_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932947.1"
                     /translation="MQTQRASERPTSWWLKTLERRRERMHSEYLDLSEPVSWASPEEQ
                     AAAVKFFNAAFRAEESGLR"
BASE COUNT         1485 a         2907 c         2937 g         1513 t
ORIGIN      
        1 gcgaacgtcg ccttcgtaga gaaagcgagc gcttggccgt tcgcgacgag cggcgcaagc
       61 cacgcgagat ccgccgacgc acgagcgtcg gtggcgacca aaacgagcgc tgcgcggcgc
      121 gcgcgaaccg cctcttcgac ggcactcgcg cccgcttcga gcttgtgggc acgacgcgcg
      181 gccaagacga gcccctggac gcgccgctct gccgcggccc gaaacgtcgc aaggacttga
      241 gtaccgttgt cgcggacctg gcacttgaat gcgcgggaca ggccttgggg ggcggccttt
      301 tcgatgcact ccggacgcgg gtggacccag gcgccgcgac cgaaagctcc gcgagcgagg
      361 tcgaccacga cctcaccctc gggacccagc acgaggcgca cgagctcacc gggatcgacc
      421 ggcgagcggc atccaacaca cgaccgctgc attcgcttca gctctcctcc tcgctgactt
      481 cttcctcgtc agcgccgacc gaagcggcca cgtccgcctc ggcttgcgcg ttgagctcgg
      541 cgcgttgatc cgccaagtgc tcccgcgcag tttgaatttc cttcgcgacc gtgcgctgga
      601 agtcgaccgc gccttgccaa acctggcgcg ccttcttgtt gccgaggccg gacttgatgg
      661 cgagccgatc ggggtcttcc ttggccacgt cgcgcacgct cttgtagccg gcctcttcga
      721 gcgcctgcgc ggtgcgcgtg cccatgcccg gcacgaccaa cagcgcgtcg tggtcgctga
      781 gcgcgccgct ctgctccgcg atgcggcgga tgcgctcttg gcggagacgc tcgagcgccc
      841 gctcggccgc ctgctgaagc tccgaagccc gcgcctcgtc ctcgacaccc tcgatggtgg
      901 cgagctcggc gagctccgcc tccgcgattt cgtcgagggc gcggaacccg aggccgaaca
      961 ggttcttggc gagctcctcg tccatgccct cgatttctgc gagctggatc atggcctcgt
     1021 cctccatctg gcggaacttg ctctcgctca cgatgtcgag gcgccagccg gtgagctggg
     1081 acgcgaggcg cacgttctgc cccttgcggc cgatggcgag cgacagtttc tcgtccggaa
     1141 ccacgagctc catgcggtgg ttggcctcgt cgacgacgac gcggttcacc tccgcgggct
     1201 ggatggcgtt gatgatgtag cgcgccggat ccttgtcgta cggaacgatg tcgatcttct
     1261 cgccgcggag ctcttgcacg actgcctgca cgcgcgaacc cttgatgccg acgcaggcgc
     1321 ccaccggatc gacgtccggg tcgcgcgagg tgaccgcgat cttgctgcgc gagctcggct
     1381 cgcgggcgac gctcacgatc ttgacgatgc cctcgtagat ctcggggacc tctgcctcga
     1441 acagcttctc gacgaggtgc ggcgactggc ggctcaagat gatcatcggg ccgcgcgact
     1501 cgcggtcgat gttcttcacg taggcgacga tgcggtcacc ggggcgatag gtctcgcgag
     1561 gagtctgctc gcgcgccggc agtacgcctt cggtcttgcc gagatcgacg atcaggttat
     1621 gccccttctc gaagcgacga acgatgccgc ggatgagttg gccctgacga tctttgtatt
     1681 cgttgtggat gatgtcgcgt tcggcgtcgc gtacgcgctg cagcagcacc tgcttcgccg
     1741 tctgcgcagc gatgcggccg aactggctgc gcgcctgctt catgtcgagc acggaaccga
     1801 actctttgtc ctgctgacgc gcgcgctcgg cgtcgcgagg gtgccaaaac acctgaaagc
     1861 ccagctcttc accgatgtcg gcgtcgaggc cgtgcttgcg agcctggtcg acggcgattt
     1921 cgcgctcggg ttgcgcgacc gcttcgacga ccgtcatgta ctggaagagg tcgatgttcc
     1981 cggtatcgga gttgtagcga gcttcgagct cgcgattcgg tccgaacgcc gcctgcgcag
     2041 ccttcaagat cgcagcttcc atcgtctcga tgagaatctt cttatcgatg cccttctctt
     2101 gggcgacttg ttcgacgatg gagcccagac cttctgtgta acccgcagct tgtgccatga
     2161 cctaacctca acgttgcgga cggtgggcac gagccccgcg tcccgatgcg cgccctggcg
     2221 accccttgcc gccgcgagcg cccgcgccac tctgaaaaac gagtcgcgcg ctttcgatcc
     2281 catcgagaga aaggggaacg agctcccctc gctcggactc gagcaggacc gcgccctgct
     2341 cgtctagccc gtgcaaggtg ccgcgcagga cgtgctgccc ctcgatgggt gccttcagtt
     2401 tcagccgggc aaattgaccg gcaaatcgtg cgtagtcgcg cgccgtgtag agcgcacgct
     2461 cgaggcccgg agagccaacc tcgagccgat agcgatgcgg aatcgcgtcc gcgacgtcga
     2521 gcgccgccga caggtcccgc gagatttccg aacagagatc aattgtgacg ccctcgcccg
     2581 ggacgcgcga gtcggggcgt tccaccgtga gctcgagcac ccacccgctt cggtccgtac
     2641 gccacacgag ctcgaccgcg tcgactgcgt gcgcgttgag cacgggcagc acggcgtcga
     2701 gcactcgctt gcggtcaagg ccgctcaatt tctcgtgggc aacgttcatg cgaagcaagg
     2761 cccgcagcgg cgaggagcgc ggcgcggacg ttcaactggg cgaagaggcg tgatgggcga
     2821 aagaatgggc gttagagaaa aaaaaacgga ccctgcgaga gcgggtccgc taggtgcggg
     2881 atccacccgg tttttcgaac gcgcggagtg tagctagacg acctacccct tgcaagcgtg
     2941 gaaccctgtt cgttcgtaac cccctgccag cgcgcatagg aatggggaga gcagcacacc
     3001 ggcctgaagg ccgatgcttc ccaggcctaa cgcgcgaacc cagtcctgcc gttcgagggg
     3061 ggaagacggg cgaccctgag gcccaagagg gacgaatgag caaacgaatt ctcgtagtag
     3121 acgacagcaa ggcgatgcgg ctcatcgtgc gccgaacgct gcggcaagcg ggcttcggcg
     3181 actacgaggt ggaagaagcc tgcaacgggc tcgaagccat ctcagccatc gaaaacgctc
     3241 gcccggacct gattctatcg gactggaata tgcccgagat gaacggctac gagctcctga
     3301 aggcgttgcg cgcaaagaag aacgacgtgc ggctcgggtt cgtcacgacc gagagcacgc
     3361 cggagatgca agccatggcg aacgaagccg gggcgctgtt cctgatcgcg aagccgttta
     3421 ccgccgagca gttccaggcc gcgttgaagc ctttcttggg ctgagccgag ctacgcgaaa
     3481 ttcggcagcg tcacgaggtc gacgtgcagg atctcgtcgg agaacgccat gatttccgac
     3541 aggcaatcct cgctcggccg agacccgagc cggatcttcg cgcacgcggc gcgcccgcct
     3601 tcgaagacgg tgttgtcgag ctcgtggatg tcgatgccgt ggcgcttgag gacggcgagt
     3661 acgttcgcca gcgctccaac gcggtcgatg tggcgcacga cgagctggta gcgtgaccaa
     3721 aacgcggcgc tgatgttcac cacgttgggc acctcgccct tggtgatgaa gcagcgcagg
     3781 atgcgagcgc actctgccgc gatcgccgtc tgcgcttgat ccgtcgacgc cccgatgtgc
     3841 ggagtcgcgt acacgaactc acttttcaag atcggatgct cgtaggttcc gctgcgagag
     3901 ccgggctcct tgggaagcac gtcgaggccg acgcgcaggc gcttcttggg gatgagctcg
     3961 gcgagcgcgt cgtagtcgac gagctcggga cgcgcggtgt tgatgaacat ggcgtcgtcc
     4021 ggtagcgcct cgatgacgcg ccgatccacg atgccgcgcg tgcgctcggc gagctcgagg
     4081 tgaaccgtga agatgtcgct ttcgcccgcg agctcttcgg gtgagcgcgc gatctgcact
     4141 ccgagctcct gggccttcgc gcccgtcagc gagcggccga aagcgtaaca cttcatgttg
     4201 tacgcctgcg cgaggcgcag cacggcccgg cccacgtgac ccagaccgag gatgccgatg
     4261 cgcttgcccg acaacccgac cgcgcgcgcg tactcgtctt tttcccagcg ccctgccctc
     4321 aagctcatga ccgcgtccgg tactcggcgg tcgaggctgc caatcagggt gaaggtgagc
     4381 tcggcgaccg cggccgcgtt cttgccgggg caattggcga cgtagatgcc gcgctcgctc
     4441 gccgccacca cgtcgatgtt gctgacgccg gctccagctc ggatgatgag gttcagggcg
     4501 cggcccgccc ggagcgcagc ccggctcacc tcggtaccgc ggacgatgag gacgttcacg
     4561 ccctccagcg cctcgggcag cttttcaggg cgtagctcag ggtcgtaagt gagctcgact
     4621 cccagtgagc gcatctcgtc gagactgtct tccgtgagcc gatcggctac caatactctc
     4681 atcgttgatc acatctcgga aggagccatg agctcgcgtt cttggccaac actcgcatcg
     4741 ggcggcccct tctatcatgc ttttcgtgac gaaatgggta agcgcacgcg ttgcccggcg
     4801 aaatccagtg ccagggcacc gtcgccgtct tcggccagca gtggggcgag cgcgagctgg
     4861 gcggcgcgcg agaaacgcgc ctggaacgcg ccgcccttga ctgccgcgta cacggcccca
     4921 ttcggcccga tttcgagcga ctcgggcccg agatcctcca gcgtaccgtc cgagagctcg
     4981 acctgaaggc agccggaggc gcgactgtcg tcagggagta tgagcgatat cacctgaaac
     5041 ggcgcgtctt ctacgcggaa atagctccag tcgtagccgt tgttcaagat gaagcgcccg
     5101 tcgtcggggt gtcgagcgat ccacgaagaa aacgcgcgcg ccatcccggg atgggtcacg
     5161 ggcgctccat cgtgaaagaa tcggccttcg ctatcgagca cgatggtgct ctcgcgcgag
     5221 cgtccttccg gcggcggtaa ccggaaaaat tctgggtggt cggagggctt cattgccgcg
     5281 cgcggagggg ccacgactca tccgatggcg tctctgattg agctccttcc gatgcagcgg
     5341 tggacatcag tgcccgaagc gcgtcggacg ggccgaaaca aaggagtctg tccccgcgca
     5401 acagctgaaa gtcgaggccg ggagtcggta cgacgcgcgc gccgcgcgtc acggtcagca
     5461 cgaggacctt acgggacgag agctcgagcg cggcaagggt gcgctgttcg aggccagacc
     5521 ccggcccaac gaccagctcc gctgtgccgt gcgtcggcgc cgcgcccagg cgctgcctca
     5581 gatcgatctc tggaaactga atctgctctt ccatgtgctg gacgatttcg cctgcaacgt
     5641 cgatgccggt cgcgctctcg atgccttcga gtcctggaga cgagttcact tcgaggacga
     5701 gcgggccatc gtcactttcg atgagatcga cccccgcgac gcgcagaccg agaatctgag
     5761 cggatttgag cgcgagcgcc tcgagctcgg gcgacaaccg cgctcgctcg gtgcggcctc
     5821 cccggtgaac gttgctgcga aattcgtcgg gcgccgcgcg tctccgcatt gcagctacga
     5881 cccgatctcc caccaccagc gcgcgcaagt cgtggccacg gctctcgctc acgaacttct
     5941 gaacgatcac gtcctggcgt gcgacgagaa acgcctcgac gatcgcttcg gccgtcttct
     6001 tcgtgtccgc caggatgacg cccgccccct gtgaaccttc ggtcaatttg atgattaccg
     6061 gtgcgccccc gacttgagag agagcactgc gcgcgctctc tcggtcgcgc acgaaggcag
     6121 tgggaggaaa cgcgatttcg tgagcgctca agatttggaa ggcgcggagc ttgtcccgcg
     6181 acgttccgat cgcttgcgcc gagttcagcg tgaacacgcc catctgttcg agctggcgca
     6241 ccacggccgt accaaaagag ctcaccgagg cgccgatgcg agggatggcg gcgtcgtagc
     6301 gagacaggac gcggtcgcga taatagagtc ggggctgcgc tcgttcgacg agcaagctga
     6361 agttcagcgt gttcagcacg cgagcagagt ggccgcggcg cacgcacgct tccttcaagc
     6421 gttgtgtgct gtaagaacca gcgatcctgg agagaatgag aaccttgagc gacatcgtga
     6481 agcgtgcgtg agtcgatatt aatgccggct gcgccgagcg ggcgcaggct ttggcttgcc
     6541 cggggagact gcacgccggt gtcccgtgag cagatatcgc cgggctggat cgacgaagaa
     6601 gtgggagccg agcgcagtac gacccagcag catgcggtag agcatgttgg cgcgatccac
     6661 taggccgagc tcgatgcgct cggtaaatgg gccgatctga atcgttgctt ccacgaaaac
     6721 gcgcgtcgcg gagacacctg tgctcgcttt gacgcgcgcg cgccgtacga tgggggcctc
     6781 gacgctgacg cgccggtcca ccttggagcg gtgcagacgc acgtcgaacc gcacgcgatc
     6841 tcggccaatt tcgcgcaagt tctcgacgtg gagcgcgctc gaacgcgccc cggtgtcgac
     6901 cttcgcgcgg agcccgcgca cgcgccactc ggcgatatcc acgtattcgg tcaagccaat
     6961 cacgaggcgt tcagcgcgcg ctgacctcac actcgaaccg tagccggttt ccgtcccaat
     7021 gtggcgttca cgcgattgcc acggtgaacg gccaccgggc cgccgattct cgaatgattt
     7081 tgcgtggccc ctggctggac gcgccggcgt cgggcgccta gagagcgata acggcgccgg
     7141 agcgcgccgg ggcgtaactt gcaggaaaat ccgccgtgac cgacgagttt cccaccctgg
     7201 aggtcgtgtc tgcagtttgc gtgtcgtggc tcgctttcag ggactgggtg ctgctgcgcg
     7261 gcgcctcgcg actgaacgac gacatcgcgc gcgtcgcgcg ccgactcgag caaaacgaag
     7321 ccgggaacga gcgcggcgcg gccccgtggc ttcgaccagt cacgaggacc gtgaacgcgc
     7381 tcttggcggg cgagatcggg acgaccaccg cgcgcgagct cttgatcgaa cgctctcttc
     7441 gcgcgcgccg caagctgacg agcggcgccg cgcgcgacct cgtggtttgc gcggtgctgg
     7501 cgggcgcgct gatttacgcg cgagcggcca agctcgaggt gggctcgccg ttcttcgggc
     7561 tcggcatcgc agcgctcgtc atgctgcttg caggcgcggc gttgcgggtg cggctggaac
     7621 gcgtcctcgg ccgagccgga gcgcgcgtcg cggaggccat cgcccgtcgg ccgagcgcgg
     7681 ccgcgagtgg tgccgcaccg cgctgtcgta tctgtcgcga gcccaagctc tcgcgcgtcg
     7741 cctcgccgga aggcctcgga gcgcaattga gcgcgctcgg cgtggtcgaa gtcttcgtct
     7801 gcccgcgctg cgggcacgtc accggcgccg ctcacccccc gagccaaacc aaagtctagc
     7861 cacgctggcg gctcggcgcg gcccgacgtg gtaaaaggcg cctcacgcat ggacgcctcg
     7921 ggcagccacg actcgaaccg ccgttattac gatgcctttt ccgcgcgcta cgaagcgcag
     7981 cgaggcgaga acgatcccgg cggctatcac gagctcatcg actcgctcga gaccgcgttc
     8041 gtggagcgct atggccgcgg ccgggaggtg ctcgaggtgg ggtgcggcac cgggctcctg
     8101 ctccgccgaa tccgagactt cgcgagcacc gcccgcggcg tggatctctc accgggcatg
     8161 ctggcgctcg ccaaggagcg ggggctcgac gtggtcgaag gttcggcaac cgccctgccc
     8221 ttcgccgacc aaagcttcga cgtgacgtgc agtttcaagg tgctcgcgca cgttcgagag
     8281 atcgagacgg cgctcgccga gatggcgcgc gtcacccgac cgggcggaca cgtcatcgcc
     8341 gaattttaca acccaatgag cctgcgcggg ctggtcaagc gtttcggtcc ggccgggtcg
     8401 gtagcgcacg acacgcacga aaagcaggta ttcacccgct tcgactcgcc gcgccgcgtt
     8461 cgagcgctca ccccggcggg ttgtcgccaa gtcgctgcgc gcggagtgcg catcgccacg
     8521 cccaccgcgc acttgatgaa gacgcgcctc ggccgcgaag tgtttcgacg cgccgaggag
     8581 ctgctctgcg actcaccgct gaaggtattc ggcggcttct acgtggtcgc gttcgaaaag
     8641 cagagctgag ccgtgcagac ccagcgagcg agcgaacgcc cgacttcgtg gtggctgaag
     8701 accctggagc gccggcgcga gcgcatgcac tcggagtacc tcgatctgtc cgaacccgtt
     8761 tcgtgggcga gcccggagga gcaggcggcc gcggtgaagt tcttcaacgc cgcgtttcgg
     8821 gccgaagaat cggggctccg gc
//
LOCUS       DASTKS010000940         5422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1042911, whole genome shotgun sequence.
ACCESSION   DASTKS010000940 DASTKS010000000
VERSION     DASTKS010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042911"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFQ35_19725"
     CDS             <1..754
                     /locus_tag="VFQ35_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9932948.1"
                     /translation="AMRAAGGSVELVDALLDRDESWGVALLRPPGHHARPGTAMGFCL
                     LNNVAVAAAHARARGIERVLVLDWDVHHGNGTQEIFYADPNVLYVSLHQSPYYPGTGA
                     DSEMGSREGLGYTVNIPLTGGATNDVYVDAFRRLVLPIVDQYRPGLALVSAGFDAHVR
                     DPLGGMALTDAAYAWFTKELRGALPSSTRLGLLLEGGYDLSALESALGACLAVLAGAD
                     APVPKSTPLTHREHERDLARAYAFQRRHWHLD"
     gene            924..998
                     /locus_tag="VFQ35_19730"
     tRNA            924..998
                     /locus_tag="VFQ35_19730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:956..958,aa:His,seq:gtg)
     gene            1712..2272
                     /locus_tag="VFQ35_19735"
     CDS             1712..2272
                     /locus_tag="VFQ35_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932949.1"
                     /translation="MLRRSDWVIALGALFGIGCSGPVETAVGAGLQLNVSTTGSCGVA
                     NPQPPDDLGGPPPDHTKANPGKVVFNGEKGVLASCSVKGSGPWQITGSVTSTDSPRVG
                     FILHSGTINADGTGTADITLSTAAIGASVSSQAGQPCQIEAVKIKDQLMVQPNAVWAT
                     FNCPNMFAPPSQTCFVAGEFLFDHCP"
     gene            complement(2452..3318)
                     /locus_tag="VFQ35_19740"
     CDS             complement(2452..3318)
                     /locus_tag="VFQ35_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9932950.1"
                     /translation="MRAVAAGITDVGLQRDHNEDSFAILKEHALYIVADGMGGHRAGD
                     VASRLATEAIVDFFRTTATEDFTWPFHFDARLSEEENRLLTGIKIANRQIIDRSNRSR
                     EYQGMGTTVVGALFNGKRGKMYIGHVGDSRAYRVRNGQIVQMTRDHSLVNDYLLAMPE
                     LTEEQRSELPKNVITRALGMQDQVAVDLQGDDAQIGDVYVLCSDGLSGMIDDAEICHV
                     VNSSHDLEEACRRLVALANEHGGEDNITALIVRVENDAETQRFSDTLPIVERKDEQTA
                     DTPPAGSTATKP"
     gene            complement(3617..4174)
                     /gene="frr"
                     /locus_tag="VFQ35_19745"
     CDS             complement(3617..4174)
                     /gene="frr"
                     /locus_tag="VFQ35_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9932951.1"
                     /translation="MIDEVLSELKKATESSKEALKRELAKLRTGRAHGGMLDSIKVDY
                     YGTTTPIAQMATVSVPEPRMITVKPWDKSQVQAIEKAIRESGLGLNPQSDGELIRIPI
                     PPLSEERRKDLVKVARKHGEEAKVGVRKSRHEALDMLNEIKDNGDASEDDVERGKKKA
                     EETVAEAVTGIDHIIAAKEKEILTI"
     gene            complement(4299..>5422)
                     /locus_tag="VFQ35_19750"
     CDS             complement(4299..>5422)
                     /locus_tag="VFQ35_19750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9932952.1"
                     /translation="TLGQLVAIKFIHPRLTNSVEALRRFDTEAKAAARLKTRHAVAVY
                     DHGVTADSEPYIVMEYLEGESLEHMIRRRGRLPFPEVTAIIAQAARALTAAHEQGIVH
                     RDLKPDNIFLAHERDAGQLGYVVKLVDFGIAKIVEEDAARGAGTTQAGMVLGTPHYMS
                     PEALTASAPVGPAADVWSLGACAFAAACGRVPFEGDAIGDVVLKVCAAPLPVPSKVAP
                     ELPHAFDEWFKRACARRLEDRFASIAEAAQALAALESWNQARRESVAFELAPNRASQA
                     VITIDEPRASRRGLVLGGVLVGASGMLAVLGYYVMQRTRAADEAAAAVAASARALIDR
                     ENERKLRESEAAFWARQSDAGEGARDGGTPAPRKKKRPR"
BASE COUNT          904 a         1810 c         1716 g          992 t
ORIGIN      
        1 ggcgatgcgc gccgcgggcg gctcggtcga actcgtcgac gcgctcctcg atcgcgacga
       61 aagctggggc gtcgcgttgc tccgtccgcc cgggcatcac gcgcgtcccg gcaccgccat
      121 gggcttctgc ttactgaaca acgtcgccgt cgcggccgct catgcgcgcg cgcgcggcat
      181 cgagcgcgtg ctcgtcttgg actgggacgt gcatcacggc aacggcactc aggagatctt
      241 ctacgcggat ccgaacgtgc tctacgtctc gttgcaccag tctccgtatt atccaggaac
      301 cggcgccgac agcgagatgg gaagccgcga gggcctcggc tacacggtca acatcccgct
      361 caccggtggg gcgacgaacg acgtctacgt ggatgccttt cgccggctcg tattgccaat
      421 cgtggaccag tatcggcccg ggctagcgct ggtgagcgct gggttcgacg ctcacgtacg
      481 cgacccgctc ggggggatgg cgctgaccga cgctgcgtat gcctggttca cgaaggagct
      541 gcgcggtgcg ctgccgagca gtacgcgact cgggctgctc ctcgagggcg gctatgacct
      601 cagcgcgctc gagagcgccc tcggggcttg cctcgctgtg ctcgcggggg ccgatgctcc
      661 cgtgccgaag tcgaccccgc tgacccatcg ggagcacgag cgagaccttg cccgggcgta
      721 cgcttttcag aggcgccatt ggcacctcga ctgagcgaaa tcacgggccg gccccatcgg
      781 aaaattccgc agaaaacggc gatcacggcg ttccctcgag ccagcagcgc aatgaatgag
      841 ttgcaggccc cccgcccctt gcgctaaatg cggcgctcct tgggaacggg agccatccct
      901 ttcccgaggc gcaagacccc gtggtgggtg tagctcagtg gtagagcact ggattgtggc
      961 tccggttgtc gcgggttcga tccccgtcac tcaccccaaa aacgaacccc gcagcgcgca
     1021 atcgctgcgg ccgaagaggc taggaatcga ttcccgaggc aaactcggga gccggttcct
     1081 gaacctcgcc gttcgctgcg caaaaccgtc gagccgctcg gaacccgccg gcatcacctg
     1141 accgggcctc gccttccttt tcggaccctt cggtcgacat tggatagtac gagggcgcac
     1201 gcttcggcgc ggagagcccg ggcgcgcagc gagcggccgc tcaagtccac catcgaaagc
     1261 gcgcccggtg cgtgaagcgc cgaaggtgcg caaaagcgag cctcgcgcag tagcagcgac
     1321 gacgcatcca atgttgcccg gctccacgct cgagggaagc acggcccagg acgttccccg
     1381 cgagccgccc ttcgtcgagc tcagccgacc acaaagcgaa cgcgcgatga gaaagttggt
     1441 gggagagaca cgtgagctgg cacctccgga agagaagcct gtgagcgcat ccaagcaaga
     1501 caggcgcgtt ggcgtgccct ccgagtcatg gcgcgtcgac gcgagtagac gtcatgtgcg
     1561 acagcgtgac cgcatacgtt cgatgaactg cgaagcgtgt ggtcacgtcg cgctggcttc
     1621 actgactctt tttcgaatga ctgacgttta gttcaaataa gtgtggatcg cgctctcgaa
     1681 aactcggaga ctgacttaga cggaggaact gatgttgcga cgttcggact gggtaatcgc
     1741 tctcggcgct ctttttggca tcggctgcag cggcccggtc gagacggcag ttggtgccgg
     1801 gttgcagctc aacgtgagca cgactggctc gtgcggagtt gcgaatccgc agccgccgga
     1861 cgacctgggt ggccctccgc cggaccacac gaaggcgaat cccggcaagg tcgtcttcaa
     1921 cggagaaaag ggagttctgg catcctgctc cgtcaaaggg tccggtccgt ggcagatcac
     1981 ggggagcgtc acgtcgaccg actcaccgcg cgtcgggttc atcctgcact cgggcaccat
     2041 caacgcagat gggacgggga cggcggacat caccctgtcg accgctgcga tcggcgcttc
     2101 ggtatcgagc caagctggtc agccctgcca aatcgaagcc gtgaagatca aggaccaact
     2161 catggtgcag ccgaacgcgg tctgggcgac gttcaactgc ccgaacatgt tcgcgccacc
     2221 gagccaaact tgcttcgtcg cgggcgagtt tttgttcgat cactgtccgt gagctagcgc
     2281 tcgaaccaac gagggcgcgc ttcctccaac acggcggcgg agcgcgcccg tttcgcgacc
     2341 gcgtcgaggg ctcgctcccg ctagcagcag tttcgcgttc gcggcgcgaa cggatgcggc
     2401 gcgtgacggc gcacagagcg cacgctagct cgcactggtg ccccgcgccc ctcagggctt
     2461 cgtcgccgtg gaaccggcgg gcggcgtatc ggccgtttgc tcgtctttgc gctcgacgat
     2521 cggcaacgtg tcggagaagc gctgagtctc ggcgtcgttc tcgacgcgca cgatcagagc
     2581 cgtgatgttg tcttccccgc cgtgttcgtt ggcgagcgca accaagcgac ggcaggcctc
     2641 ctcgagatcg tgagagctgt tcacgacgtg acagatctcg gcgtcatcga tcatgccgct
     2701 caagccgtcg gagcaaagta cgtagacgtc accgatctgc gcgtcgtcgc cctgcaaatc
     2761 gacggcgacc tggtcctgca tgccgagcgc gcgcgtaatg acgttcttcg gcaactcact
     2821 ccgctgctct tcggtgagct cgggcatcgc gaggaggtaa tcgttgacga gcgagtggtc
     2881 gcgcgtcatc tggacgattt gtccgttgcg aacacggtag gctcggctgt cgccaacgtg
     2941 gccgatgtac atcttgccgc gcttgccgtt gaacagcgcc ccgacgaccg tcgtgcccat
     3001 gccctggtac tcacgcgacc gattgcttcg atcgatgatc tgacggttcg cgattttgat
     3061 cccggtgagg aggcgattct cttcctccga caggcgcgcg tcgaagtgga acggccaggt
     3121 gaagtcttca gtcgcggtgg tgcggaagaa gtcgacgatg gcctccgttg cgagccggct
     3181 cgcgacatcg ccagcgcgat gcccgcccat gccgtccgca acgatgtaga gcgcgtgctc
     3241 cttcaggatc gcgaagctgt cctcgttgtg gtcgcgctgc agaccgacat cggtaattcc
     3301 tgctgctacc gcgcgcatcg aacccagtcc ttttgaggag ccgacccgtt aattagcact
     3361 gacgaaaaaa gtttcatggt aagggcagcc tgtcaagctc gcgaccacgc tttatcccgg
     3421 ccattcgagc cgttagccgt ggcccgaggg acgcccggct ctcatgcagg actcgggctc
     3481 acaggctcgg cggccagcct tttcgacacg agcagggtcg ccacgctccc cctcggcctc
     3541 gtgtcgacgt tcgctcccgc cccgggcacc gccgaagctc ggcgattcgc cgccgcacgc
     3601 ctcgaagagc gaggcgtcaa atcgtgagga tctctttctc tttggccgcg atgatgtggt
     3661 cgatgccggt aacggcctcc gcgaccgttt cctcggcctt cttctttcct cgctcgacgt
     3721 cgtcctcgct cgcgtccccg ttgtctttga tctcgttcag catatcgagc gcctcgtggc
     3781 gcgacttgcg cacgcccacc ttggcctctt cgccatgctt acgagcgacc ttcacgagat
     3841 ccttgcgccg ctcctcgctg aggggcggaa tcggaattcg aatcaactca ccatcggact
     3901 gcggattcaa gccgaggccg ctctcgcgaa tcgccttttc gatcgcctgc acctgagact
     3961 tgtcccaagg cttgacggta atcatgcgcg gctcggggac gctcacggtt gccatctgcg
     4021 cgatcggcgt cgtcgtcccg tagtaatcga ccttgatgga gtccaacatg ccgccgtgcg
     4081 cacggcccgt gcgaagcttc gcgagttcgc gcttcagcgc ctctttcgaa ctctcggtag
     4141 ccttcttcag ctcggaaagg acttcgtcga tcatcctgca cccatcgctt ggtagaacgg
     4201 ccggaagacg ctcctaggcc gggcggggga tataacccga gacacgtcgc ggttccacgc
     4261 cttccccaac cgagctcgca gttttcagcc ggcgcggctc agcgcggccg cttcttcttg
     4321 cgcggcgctg gggtaccgcc gtcccttgct ccctcgcctg cgtccgattg tcgcgcccag
     4381 aaggctgctt ccgattcgcg cagcttgcgc tcgttctctc ggtcgatgag cgcccgcgcg
     4441 ctggcggcca ccgccgcggc cgcttcatcc gcggcgcgcg tccgctgcat cacgtagtag
     4501 cccagcacgg ccagcatccc gctcgcgccg accaacactc cgcccagcac gagccctcgc
     4561 cgcgacgcgc gcggctcatc gatggtgatg acggcctgcg aggcgcggtt cggagcgagc
     4621 tcgaacgcga cgctttcgcg gcgagcctgg ttccaagact cgagtgccgc gagggcctga
     4681 gcagcctcgg ctatcgaagc gaagcggtct tccaagcgtc gcgcacaagc gcgcttgaac
     4741 cattcgtcga aggcatgggg cagctcgggt gcgaccttgg aggggaccgg taacggcgct
     4801 gcgcaaacct tgagcaccac gtcgccgatt gcatctccct cgaacggcac tcgcccacat
     4861 gcggccgcga aggcgcaagc gccgagtgac caaacatccg cggcagggcc aaccggcgcc
     4921 gaagcagtga gcgcttccgg actcatgtaa tgcggagtgc cgagcaccat ccccgcctgg
     4981 gtcgtccccg cgccgcgagc cgcgtcttcc tccacgatct tcgcgatgcc gaagtcgacg
     5041 agcttgacga cgtagccgag ctgcccagcg tcgcgctcgt gcgcaaggaa gatgttgtcc
     5101 ggcttgaggt cgcgatgcac gatcccctgc tcgtgcgcag cggtgagcgc gcgcgccgct
     5161 tgcgcgatga tcgcggtgac ctccggaaac ggcagccgtc cgcgccgacg gatcatgtgc
     5221 tcgagggatt cgccctcgag gtactccatc acgatgtacg gctcgctgtc ggccgtcact
     5281 ccatggtcgt aaaccgctac cgcgtgtcgc gttttgagcc gcgcggcagc cttggcctcg
     5341 gtgtcgaagc gccgcagcgc ctccacgctg ttcgtgaggc gcgggtgaat gaacttgatg
     5401 gcgacgagct gccccagtgt ta
//
LOCUS       DASTKS010000941         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_150653, whole genome shotgun sequence.
ACCESSION   DASTKS010000941 DASTKS010000000
VERSION     DASTKS010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150653"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFQ35_19755"
     CDS             complement(<1..1040)
                     /locus_tag="VFQ35_19755"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9932953.1"
                     /translation="MLVSEIEVLALDVDGVITNGSVTLGAHGAETKGIAMRDLDAITA
                     ARRAGLRIALVTGESSAMVDVIAARIGVDCVVSGAKDKRVGLIQVCDRLAVTSDRICF
                     VGDADRDALAFSSVGLSFAPADASARARAKASRVLTSRGGQGAVAEVVEILLAARSAR
                     AQSGMIERQLRRVVEESIAAHQKLLEESLPTLGSIATLIADCLRGGGKVLFCGNGGSA
                     ADAQHVAAELVGRFAIEREPFPAIALTTDTSILTAVGNDWDFRDVFARQVRALARPLD
                     IVVGISTSGRSPNVVRALEAAREKGATTLAFVGRNGGPIAKAADVVFKAPAAVTARVQ
                     ELHILAWHGVCE"
     gene            complement(1031..1879)
                     /locus_tag="VFQ35_19760"
     CDS             complement(1031..1879)
                     /locus_tag="VFQ35_19760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9932954.1"
                     /translation="MSASPGVSGALARSFDLSERVFVITGGAGLLGVEHAHAIAEAGG
                     LPVLADVREDTARERAEEVTRRVGRRVEAVPLDVTDKASCEAALSRVLSEFGRVDGLV
                     NNAARNPKVEGGGLAASRLENFSLETWNQDIAVGLTGTFLMSQVFGTYLARQRRGVIV
                     NIASDLGVIAPDQRIYRKPGLPEDEQPVKPITYSAVKGGLVMLSKYLATYFAEQGVRV
                     NALVPGGVYAGQPDDFVERLSALIPLGRMARADEYRAALVFLCSDASSYMTGASLVVD
                     GGRTCW"
     gene            complement(1858..2616)
                     /locus_tag="VFQ35_19765"
     CDS             complement(1858..2616)
                     /locus_tag="VFQ35_19765"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HET9932955.1"
                     /translation="MRPEVLALIPARGGSKSVPRKNLLKVAGKPLLAYSIAHAQGCPS
                     VTRTIVSTDDLEIADVAQEFGAEVPFMRPPEYATDFATDFEVFRHAIEWLAEDEHYVP
                     DLIVHLRPTGPVRDVALIERAIQLMLKHPEADSLRSVGLAEQTPYKMWRIEDSYLRPL
                     VELREFPEAHSMPRQKLPAAYWQNGYVDIVRPRTLLELDSMVGRVVLPFVVEGKIHEL
                     DYPEQIPALEAAVSAWERGERPSPEETACPRHPA"
     gene            complement(2616..3704)
                     /locus_tag="VFQ35_19770"
     CDS             complement(2616..3704)
                     /locus_tag="VFQ35_19770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9932956.1"
                     /translation="MTGESGPKPAPISRALFVGLGGIGQRHLRNLRALFGDSVAVDAF
                     RVRREQRVLDDQLSVIADSDLDARYGVSVYSDLDAALAQGPDAVFVTNPSSMHVDVAR
                     RAAEASAHVFIEKPVSHSLEGVLELDALLRRKQRVGFVAYQLRRHPGFRKLSERLAEG
                     AIGRVLSVRAEVGEYLPNFHPYEDYRRMYASRRDLGGGVTLSQIHELDYLIALFGKPR
                     RAFAMGGKVSSLEVDVDDLTSGVVEFRGPRHGRVIVEVHQDFFSRPTSRRCVVVGDAG
                     RLEWSLSERSFRRWDASATLVEQDDYAAYPRNEAFLEELRYFFDCIRERKQPDVDVRV
                     GAESLKLALGLLESQSSGRAIEFEENTR"
     gene            complement(3701..4543)
                     /locus_tag="VFQ35_19775"
     CDS             complement(3701..4543)
                     /locus_tag="VFQ35_19775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET9932957.1"
                     /translation="MKHHIGIMQGRLSPRPVGKLQAFPHNCWQREFSLAARLGFDAIE
                     WIFEADRSAENPILTASGRAEIVRASRSSGVAVRSVCGDYFMIERLAGAGRDANLRNR
                     SVLRELIQQAAELGAERILLPLLETSALPTAELKDEIVECLQAVAPVAEEYRVVLGLE
                     MEIPGEEYADLIARVGHPMVKAYYDVGNSTAQGFDVATDVVPLLPKLHAVHIKDRRTH
                     GVSVPVGRGDANFPGFFRALKSAGFVGDLLLQHFFDDDPEGAARAALAYVRRELAAAG
                     EAAA"
     gene            complement(4568..5434)
                     /locus_tag="VFQ35_19780"
     CDS             complement(4568..5434)
                     /locus_tag="VFQ35_19780"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET9932958.1"
                     /translation="MPQVKIGNSIVGDGAPCYIVGEIGINHNGDLGIAKRLIDVSAIA
                     GANAVKFQKRTIEVVYSADELGRPRENPFGATNGDLKRGLEFGLDQYRTIDRYCSEKG
                     IAWFASCWDEASVDFMEQFNPPAYKIASASLTDDNLLRHHRRTGRPIIVSTGMSTMAQ
                     VDHAVSVLGSDNLVLLHTTSAYPAKIADLNLRMIPTLRERYGVPVGYSGHEVGLASTF
                     AAVALGACMVERHITLDRAMWGSDQAASVEPQGLMKLVRDIRVIEESLGDGVKKVTAD
                     EVPIMKKLRRVG"
     gene            complement(5490..>6187)
                     /locus_tag="VFQ35_19785"
     CDS             complement(5490..>6187)
                     /locus_tag="VFQ35_19785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9932959.1"
                     /translation="GGTWMNARLTGEQNYLDVRYAEASRPYTDYPGLLTRHLTERYLR
                     GYAGGRLLDLGSGRGEFLHGFAELGFAATGADRSRPKTPRFPEPVLELDYEKEPLPVG
                     DGSFDVIFNKSVFEHVSNIGAVLGECRRVLVSGGRMISMVPDWTAQFRHFYDDWTHVR
                     PFTLTGLRECIASHGFRVHTALRFRQLPVLWERPYLRPLADAATLLPEPFKRFKFVRF
                     SQEWMLLVVADKP"
BASE COUNT         1102 a         2058 c         1997 g         1030 t
ORIGIN      
        1 atttcgcaga cgccgtgcca ggcaaggatg tggagctcct ggacgcgcgc ggtcacggcc
       61 gcgggagcct tgaagaccac gtccgccgcc ttggcgatgg gcccgccgtt gcgaccgacg
      121 aacgcgaggg tcgtcgcgcc cttctcgcga gcggcttcga gcgcgcgaac gacgttgggt
      181 gagcgcccgc tcgtgctgat cccgacgacg atgtcgagcg gacgcgcgag cgcgcgaacc
      241 tgacgagcga agacgtcgcg aaagtcccag tcgttcccga ccgcggtgag gatcgacgtg
      301 tccgtcgtga gcgcgatcgc tggaaaaggc tcccgctcga tggcgaagcg cccgacgagc
      361 tcggccgcga cgtgttgagc atcggctgcg ctgccgccgt taccgcagaa gagcaccttg
      421 ccgccgccgc gcaggcaatc ggcgatgagg gtcgcgatgc tcccgagcgt cgggagcgac
      481 tcctcgagca gcttttggtg agcggcgatg ctctcttcga ccacgcgccg gagctggcgc
      541 tcgatcatgc cactctgggc acgcgcgctg cgggctgcga gcaagatctc gaccacctcc
      601 gcgaccgcgc cctgccctcc gcgcgacgtc aagacccggc tcgccttggc gcgcgctcgc
      661 gccgacgcat cggcgggagc gaacgagagc cccacgctcg aaaacgcgag cgcgtcgcga
      721 tccgcgtcgc caacgaagca gatgcggtcg ctcgtcaccg cgaggcgatc acacacctga
      781 atcaagccga cgcgcttgtc cttggcgccc gaaacgacac agtcgacgcc gatgcgcgcc
      841 gcgatgacat ccaccatcgc cgacgattcc ccggtgacga gcgcgatgcg caacccggcg
      901 cgccgcgccg cggtgatcgc gtcgagatcg cgcatggcga tgcccttggt ttccgcgccg
      961 tgcgccccca gcgtcaccga gccattcgtg atgactccgt ccacgtcgag cgccaagacc
     1021 tcgatttcgc tcaccagcac gtccgacctc catcgacgac caggctcgcc cccgtcatgt
     1081 acgacgaggc gtccgagcat aaaaacacga gtgcagcacg gtactcgtcc gcgcgcgcca
     1141 tgcgccccaa ggggatcagc gcgctcagtc gctcgacgaa atcgtcgggt tgcccggcgt
     1201 acacaccgcc tggcaccagc gcattcacgc gcacaccctg ctctgcgaag tacgtagcta
     1261 gatacttgct gagcatcacc aatcctcctt ttacggcaga ataggtgatg ggcttaaccg
     1321 gctgctcgtc ctccgggagc ccgggtttcc tgtaaattcg ctgatcgggg gcgatcacgc
     1381 cgagatccga cgcgatgttc acgatgacgc cgcgccgctg tcgggcaagg tacgttccga
     1441 atacttggct catcaagaag gtgcccgtga gcccgaccgc gatgtcctgg ttccacgtct
     1501 cgagagagaa gttctccagt cgagacgcag cgaggccccc gccctcgacc ttcggattcc
     1561 gcgccgcgtt gttcacgagc ccgtcgacgc gcccgaactc ggacaacacc cgcgagagcg
     1621 cagcttcgca ggaggccttg tcggttacgt cgagcggaac ggcctcgact cttcgaccta
     1681 cgcgccgagt cacctcttcg gctcgttcgc gcgcggtgtc ttcgcgcacg tcggcgagca
     1741 cgggcagccc cccggcttcg gcgatggcgt gcgcgtgctc gacgccgagc aggcccgcgc
     1801 cgccggtgat cacgaatacg cgctcgctca aatcgaacga tcgcgcgaga gccccgctca
     1861 cgccgggtga cgcggacatg ccgtttcctc cgggctgggc cgctcaccgc gctcccaagc
     1921 ggaaaccgcc gcttcgagcg ccgggatctg ctcggggtaa tcgagctcgt gaatcttccc
     1981 ctccaccacg aacggcagaa cgacgcgccc gaccatggag tcgagctcga gcagagtgcg
     2041 cgggcggacg atatcaacgt agccgttctg ccagtacgcg gcgggcagct tttgccgcgg
     2101 catcgagtgc gcctccggga actcgcgcag ctcaaccaac ggccgcaggt acgagtcctc
     2161 gatgcgccac atcttgtacg gggtctgctc ggcgagcccg accgagcgca gcgagtctgc
     2221 ctcggggtgc ttcaacatca gctggatggc gcgctcgatg agggcgacgt cgcgcacggg
     2281 gccagtcgga cgcaagtgca cgatgaggtc tggcacgtag tgctcgtcct ccgcgagcca
     2341 ttcgatcgcg tgacggaaca cttcgaagtc ggttgcgaag tcggtcgcgt actcgggcgg
     2401 gcgcatgaag gggacctccg ccccaaactc ctgcgccacg tcggcgatct cgagatcgtc
     2461 ggtcgagacg atggttcgcg ttacgctcgg gcagccctgc gcgtgggcga tggagtatgc
     2521 caagagcggc ttacccgcga cttttaggag gttcttgcgt ggcaccgact tggagcctcc
     2581 gcgcgcgggg atcagggcca agacctcggg tctcattagc gtgtgttctc ctcgaattcg
     2641 atggcgcgcc cgctcgactg ggattccagc aagccgagcg caagcttcag actttcggcg
     2701 ccgacacgca cgtcgacgtc gggctgcttc cgctcgcgaa tgcagtcgaa gaagtagcgg
     2761 agctcttcca ggaaggcttc gttgcgaggg taggcggcgt aatcgtcctg ttcgacgagc
     2821 gtcgcgctcg cgtcccaacg gcggaacgaa cgctcgctca ggctccactc gagccggccc
     2881 gcgtcgccga cgacgacgca gcggcgcgag gtcggacgcg agaagaagtc ctggtgcacc
     2941 tcgacgatga ctcggccgtg gcgcggcccg cggaattcga cgacgccgct cgtgagatca
     3001 tcgacgtcga cctccaagct cgagactttg ccgcccatcg cgaaggcgcg gcgcggcttc
     3061 ccgaacagcg cgatcaagta gtcgagctcg tggatttgag acagtgtcac accgccgccg
     3121 agatcgcggc gcgacgcgta catgcgtcgg tagtcttcgt agggatggaa gttcggtagg
     3181 tactcaccca cttccgcgcg caccgacagc acacgaccaa tcgcgccctc ggcgaggcgc
     3241 tcgcttagct tccgaaaacc tggatgccgg cgcagttgat acgccacgaa gccgacccgt
     3301 tgcttccttc gaaggagcgc gtcgagctcg agcacgcctt cgagcgagtg cgacaccggc
     3361 ttttcgatga agacgtgggc ggatgcttct gcggcacgac gcgcgacgtc tacgtgcatg
     3421 ctggaagggt tcgtcacgaa caccgcgtcg gggccctggg cgagcgccgc gtcgagatcc
     3481 gagtacaccg agacgccgta gcgagcgtcg agatccgaat cggcgatcac cgagagctga
     3541 tcgtcgagca ccctttgctc gcgccgaacg cgaaaagcgt cgaccgcgac cgagtcgccg
     3601 aagagcgccc gaagattgcg caagtggcgc tggccgatgc cgccgagccc aacgaacaag
     3661 gctcgcgaga tgggcgcggg ttttggcccg ctctcgccgg tcacgcggca gcctctcccg
     3721 ccgccgcgag ctctcgtcgc acgtaagcga gcgcggcgcg cgcggcgcct tcggggtcgt
     3781 cgtcgaagaa gtgctgcaac agaaggtcgc cgacgaagcc cgcgctcttc agggctcgaa
     3841 agaagccggg gaagttcgcg tcaccgcgtc cgaccggcac gctgacaccg tgcgttcgac
     3901 ggtctttgat gtgcacggcg tggagtttcg gcaggagcgg cacgacgtcg gtggcaacgt
     3961 cgaagccttg cgcggtcgag ttgccgacgt cgtagtacgc cttcaccatc gggtgaccga
     4021 cgcgcgcgat gagatccgcg tactcttctc cggggatctc catttcgaga ccgagcacca
     4081 cacggtactc ctcggccacg ggcgccaccg cctgcagaca ctcgacgatc tcgtctttga
     4141 gctcggccgt cggcagggcc gaggtttcga gcaacggcag caagatgcgc tcggcgccga
     4201 gctccgccgc ttgctggatc agctcccgaa gcacgctccg attgcgcagg ttcgcgtctc
     4261 gtcctgcgcc agcgagacgc tctatcatga agtagtcgcc gcaaacggag cgcaccgcaa
     4321 ccccgctcga gcgcgaagcg cgcacgatct cagcgcgccc gctcgcggtg aggatcgggt
     4381 tttcggcgct gcggtcggcc tcgaagatcc actcgatggc atcgaagccg agccgcgccg
     4441 cgagcgagaa ctcgcgttgc caacagttgt gcggaaacgc ttggagcttg ccgacgggac
     4501 gcggcgacag cctgccctgc atgatcccga tgtgatgctt catcgagcta ccctccggcg
     4561 tccgcgctca gccgacgcga cgcagcttct tcatgatggg aacctcgtcc gccgtgacct
     4621 tcttgactcc gtcaccgagg ctctcctcga tgacccgaat gtcgcgaacc aacttcatca
     4681 atccttgcgg ctcgacgctg gccgcctgat cgctgcccca catggcgcga tccaaagtga
     4741 tgtgacgctc gaccatgcag gcgccgagcg cgactgcggc gaaggtcgaa gcgagcccga
     4801 cctcgtgccc agagtaaccc accgggaccc catagcgctc gcgaagcgtt gggatcatac
     4861 gcagattcaa atcggcgatt ttggccgggt aggcgctggt cgtatgcaag agcaccaggt
     4921 tatcgctacc gagaacggag acggcgtgat ccacctgagc catcgtgctc atgccggtcg
     4981 acacgatgat cggtcggccc gtgcggcggt ggtgacgcag gaggttgtcg tccgtcagcg
     5041 aagccgaggc gatcttgtaa gcggggggat tgaactgctc catgaagtcg accgacgcct
     5101 cgtcccaaca tgaggcaaac caggcgatcc ccttctccga acagtagcga tcgatggtgc
     5161 ggtactggtc gagtccgaac tcgagccctc gcttgaggtc gccgttggtc gccccgaacg
     5221 ggttttcacg cggacggccg agctcgtccg cgctgtacac gacttcgatg gtgcgcttct
     5281 ggaacttgac ggcgttcgcc ccggcgatcg ccgagacgtc aatcaaccgc ttggcaattc
     5341 cgagatcacc attgtgattg atgccgattt ccccaacgat gtagcaaggg gcaccgtcac
     5401 caacgatcga attaccaatt tttacctgcg gcattttgat tgaaactccg tccttgagtg
     5461 agcccgcgag ctttcgcggg gtgaaacttt cagggcttgt cggcgacgac caacagcatc
     5521 cattcctgtg agaaacgcac gaacttgaat ctcttgaagg gctcggggag cagcgtcgcg
     5581 gcatccgcca gcgggcgcag ataggggcgc tcccacaaaa ccggcagctg gcgaaaacgg
     5641 agggcggtgt gaacgcggaa gccgtggctc gcgatgcact ctcggaggcc cgtcagcgtg
     5701 aacggccgca cgtgcgtcca gtcgtcgtaa aagtgacgga actgcgcggt ccagtccgga
     5761 accatggaga tcatgcgccc gcccgagacg agcacccggc ggcattcgcc gagaacggcg
     5821 ccgatgttcg atacgtgctc gaacacggat ttgttgaaga tcacgtcgaa gctaccgtcg
     5881 ccaaccggaa ggggctcttt ctcgtagtcg agctctagaa ccggctcggg aaagcgcggc
     5941 gtcttcgggc gcgagcgatc ggcgccggtc gcggcgaacc cgagctctgc gaagccgtgg
     6001 aggaactcac cgcgtccgga gccgaggtcg agcaaccgtc cgccagcgta ccctcgcaga
     6061 tagcgttcgg tcaggtgacg cgtgaggagc cctgggtaat cggtgtaggg gcgcgatgct
     6121 tccgcgtagc gcacgtcgag gtagttctgt tcaccggtca gccgtgcgtt catccacgtt
     6181 cctcctc
//
LOCUS       DASTKS010000942         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_150711, whole genome shotgun sequence.
ACCESSION   DASTKS010000942 DASTKS010000000
VERSION     DASTKS010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150711"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1150
                     /locus_tag="VFQ35_19790"
     CDS             143..1150
                     /locus_tag="VFQ35_19790"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9932960.1"
                     /translation="MAAAERLLCERVARLLAARRVTLVERVQELWGGYGELVRVDVER
                     SDGDEQPAVVKHVSPPRAPSSNTPEGRSHRRKLRSYEVEATFYRDFSSRCTEPCRVPR
                     HLGAEARGESRVLVLEDIDFAGFPRRKRRVGASEAEACLRWLAAFHATFLGVEPTGLW
                     KQGTYWHLATRPDELGALRFPELRRAASAIDERLRAAQFVTLVHGDAKLDNFCFSAVG
                     SVAAVDFQYVGAGPGVKDVVYLLSSCFDGEENVAFAPALLDFYFHELSRNLEDQLSAA
                     ARGALEAEWRSLYAYAWADWERFLFGWAPHEPRDRYAAAHTERVLAELNSSLRKPSSG
                     A"
     gene            complement(1113..2363)
                     /locus_tag="VFQ35_19795"
     CDS             complement(1113..2363)
                     /locus_tag="VFQ35_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932961.1"
                     /translation="MKPKLQVRLAAGRRRPVRDVRLRSRARLHAQLFAALVLFGATAA
                     PALPRLRPSDLTIGAKAWEQLGIGAMRGVTIGPIESSLHPNRGYGSPAFQRTLDEVQR
                     MGASWVSLTPFGRVLDLKPTGVAMTFEQPFEENQRAVKRAVAQAHARGLRVLLVPHLW
                     VETGAWRAEIDPGTDAAWARWSRGYLDFVRAWAEVARDAEVDMLAVGVELRSWVTTTR
                     VESFYPVLAEVRRIFPGLLTYAANWDDVEDTAILSELDVIGINAFYPLSEKNGASDEQ
                     LAFGGKDVAQRVARLAESFRKPVAFTEFGYTTRADPAVRPWEWPDFMSNVVVDQRAQA
                     DAYAALLAPMLDAPWFAGAFVWRTYADPDDLSQEAEWGFSPRGKLAELTLRDAFGARW
                     AGDGAYESGVFGFTRPSSVFVGCY"
     gene            complement(2371..3090)
                     /locus_tag="VFQ35_19800"
     CDS             complement(2371..3090)
                     /locus_tag="VFQ35_19800"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9932962.1"
                     /translation="MRRADVALETFPATLFDYNGVLADDEIVHLESFRDALAPAGISV
                     SERDYVDRYLGFDDRGAFRAILIDSGRTPTEEEILELVTVKKPLYLARAEARLPTFPG
                     ASDIVRRRAARGPVGVVSGALRDEIALGLKVLGVGEHVRYIVSAEDTRACKPDPEGYV
                     IGIEHLSAELGAELAKLALVIEDSIAGIEAAKAAGLACVAVTHSYGEAELRRAGADLV
                     VQRLDELSEEQLAALFKQLHP"
     gene            3300..3521
                     /gene="infA"
                     /locus_tag="VFQ35_19805"
     CDS             3300..3521
                     /gene="infA"
                     /locus_tag="VFQ35_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9932963.1"
                     /translation="MSKGDLLEMEGVIQDALGGGQYNIRVDQGGAIVRAQLSGRMRRH
                     HIRVLPGDRVRVAVSPYDLSHGLIVYRGK"
     gene            3518..4477
                     /locus_tag="VFQ35_19810"
     CDS             3518..4477
                     /locus_tag="VFQ35_19810"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9932964.1"
                     /translation="MSPSRLWAASLLLACLAACSRGEREGSGGSASSEPPSPPLPVAA
                     EVPSAPLALLHVPSADEALPPPIGGGLLPGAPPAARSRCPPEMVDISGQFCIDRYESS
                     YVDSASGLALSPHYHPTRAQTSASYERYRRRSSNDSLAVPEPPPFQLERDPEPEARSL
                     PGVIPQGYVSQEIAERACRAAGKRLCSLAEWVRACRGEQNRKYPYGEQYREGVCNVFR
                     EAHPAARLHGDASREHLDPRLGLVQGERGPLLRRTGETTECRSVWGNDGVFDMVGNLD
                     EWVADGGFAGGFFSRGTREGCDARITTHPGYYFDYSLGGRCCR"
     gene            4563..4970
                     /locus_tag="VFQ35_19815"
     CDS             4563..4970
                     /locus_tag="VFQ35_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932965.1"
                     /translation="MKSRAFSPEPYARLVVASVLTLFSAGCGHPVQRQLEGTWHGDAV
                     ENFEDRELARATGWAKSLRFTFSGSKMSVSIAAEEERSGNYRVRSVHNSDVVLAVKKP
                     DGAEDVARLKLDDDNSIRWMLGDSRSVVLRREL"
     gene            complement(4988..>5466)
                     /locus_tag="VFQ35_19820"
     CDS             complement(4988..>5466)
                     /locus_tag="VFQ35_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932966.1"
                     /translation="SGLPLDQAPVAVAKQSPPFASNRPLRRWTPSAPPSVSPRVESVE
                     TTPAEPALPLATSREPAPVPRRLNIGEVEQGVAQVVDAITTCFRESTPHSRTLKVTAQ
                     SAVTLSVAPDGSIEQVSFDPPLSPSVQECTEHGLAELRFAPSIEGASVTRVLELTR"
BASE COUNT          898 a         1787 c         1824 g          957 t
ORIGIN      
        1 cagtatgact tgtccattcg cgggccctta gcagggcgtt gaaaacgccc tgcgtcacga
       61 cagaccaatg gcaggcgcac acgacgcgcc gccgcacggt ggtgccggtg agcattctag
      121 cagggcgtga tatggtcccg acgtggccgc ggccgaacga cttctctgcg aaagggtagc
      181 tcggctgctc gcggcgcgcc gagtgacgct ggtcgagcgc gtgcaagagc tgtggggtgg
      241 ttacggtgag ctcgtccgcg tcgacgtcga gcgctccgac ggcgacgagc agcccgccgt
      301 cgtcaagcac gtgagtccgc cgcgcgcgcc gagctcgaac acgccggaag gccgttcgca
      361 ccggcgcaag ctgcgctcgt acgaagtgga ggcgaccttc tatcgcgatt tctcttcgcg
      421 ttgcacggaa ccctgtcgcg ttccgaggca cctcggcgcg gaagcgcgag gggagtcgcg
      481 cgtcttggtg ctcgaagaca tcgacttcgc gggctttccg cggcgcaaac ggcgagtcgg
      541 tgcgagcgag gcagaagctt gcctgcgctg gttggccgcc tttcacgcga cgtttctcgg
      601 ggtggagccc acgggtttgt ggaagcaagg cacttactgg cacctcgcga ctcgccccga
      661 cgagctcggc gcgttgcggt ttcccgagct ccggcgcgcg gcgagcgcca tcgacgagcg
      721 gctccgcgcg gcgcagttcg tcacgctcgt gcacggcgac gcgaagctcg ataacttctg
      781 cttttcggcg gttggctccg tggcggcggt cgacttccag tacgtcggcg cgggccctgg
      841 cgtcaaagac gtcgtgtacc tgttgagcag ttgcttcgac ggagaggaaa acgtcgcgtt
      901 cgcgcccgcg ctcttggact tctacttcca cgagctctcg cgcaacttgg aagaccagct
      961 cagcgcggcc gcgcgaggtg cgctcgaagc ggagtggcgc tctctttacg cttacgcatg
     1021 ggccgactgg gagcgcttcc tgttcggttg ggcgcctcac gagccgcgcg atcgctacgc
     1081 ggctgctcac accgagcgcg tgctggccga gctcaatagc agcctacgaa aaccgagctc
     1141 gggcgcgtga aaccgaacac gccgctctcg taggcgccgt ccccagccca acgcgcgccg
     1201 aatgcatcgc gcaaggtgag ttcagcgagc ttgccacgcg gtgagaatcc ccactcggct
     1261 tcttgtgaaa ggtcatccgg gtcggcgtag gtccgccaca cgaacgcgcc cgcgaaccac
     1321 ggcgcatcca acatcggcgc gagcagcgcg gcgtaagcgt ctgcctgggc tcgttgatcg
     1381 acgacgacgt tcgacatgaa atcaggccat tcccaagggc gaacggcggg gtcggcgcgc
     1441 gtcgtgtagc caaactccgt gaaggcgacg ggcttgcgaa acgactccgc caggcgcgct
     1501 acccgctgcg cgacgtcttt ccccccaaaa gccagctgct cgtcgctcgc accattcttt
     1561 tcgctgagcg gataaaaggc attgatgcca atcacgtcga gctcggacag gatggccgtg
     1621 tcctcgacgt cgtcccaatt ggcggcgtac gtgaggagcc cggggaagat gcgccgcacc
     1681 tcggcgagca ctgggtaaaa gctctcgacg cgggtggtcg tcacccagct gcggagctcg
     1741 acgccgaccg cgagcatgtc gacctcggcg tcgcgcgcga cctccgccca ggcgcgcacg
     1801 aagtccaagt agcctcggga ccaacgtgcc cacgccgcgt cggttccggg atcgatctcg
     1861 gctcgccaag ctccggtctc gacccacagg tgtggaacga ggagcactcg cagcccacgc
     1921 gcgtgtgcct gcgcgacagc gcgtttgacc gcgcgctggt tctcttcgaa cggctgctcg
     1981 aaggtcatgg cgacaccggt cggcttcaga tcgagcacgc gcccgaacgg ggtcaggctg
     2041 acccagcttg ctcccatgcg ctggacctca tcgagcgtgc gctggaaggc aggcgagccg
     2101 tagccacggt ttggatgcag cgagctttcg atgggtccga tcgtgacccc gcgcatcgcg
     2161 ccgattccga gctgttccca tgctttcgcg ccgatcgtca aatcgctggg tctgagacgc
     2221 ggcaaggcag gcgcggcagt cgcaccgaat aggaccagcg ccgcgaagag ctgcgcgtgc
     2281 agccgcgcac gagagcgtag gcgcacgtct cgaacgggcc gtcgccttcc cgccgcgagc
     2341 cggacttgga gcttcggttt caagcggccc ttacggatga agctgcttga agagcgcggc
     2401 gagctgctcc tccgaaagtt cgtccaaacg ttgcacgact agatctgcgc ccgcgcgccg
     2461 cagctcggcc tcgccatagc tgtgcgtcac cgcaacgcag gcgagcccag ccgccttcgc
     2521 cgcctcgatc cctgcgatcg agtcttcgat cacgagagcg agcttcgcga gctccgcgcc
     2581 gagctcggcg ctcaaatgct cgatgccgat cacgtaacct tcgggatctg gtttgcaggc
     2641 gcgcgtgtcc tcggcgctca cgatgtagcg cacgtgctca ccgaccccga gcactttgag
     2701 acccaacgca atctcgtcgc gcagcgcgcc cgaaacgacg cccacgggac cgcgcgccgc
     2761 gcgtcgacgc acgatgtcgg acgcgccggg gaaggtcggc agtcgcgctt cggcccgcgc
     2821 gaggtagagc ggcttcttca ccgtgacgag ctcgagaatc tcttcttcgg tcggggtacg
     2881 tccgctgtcg atcaagatcg ctcggaaagc gccgcggtcg tcgaacccga ggtagcggtc
     2941 gacgtagtcg cgctccgata cgctgatgcc agctggagcc aaggcgtccc ggaatgactc
     3001 caggtgaacg atttcgtcgt ccgcgagtac gccgttgtaa tcaaacaggg tcgcggggaa
     3061 ggtttcgagg gctacgtcgg cgcgtcgcat gagggggttt gcctgaggag cggcggggga
     3121 ggttggctga gtcgctgggc cgtatggatt cggcggccgg ccggtttggg caggagcttg
     3181 catcagccaa tcgactatgt cagcttccgc gccggccttc ggctccgcga ccccttcagg
     3241 atccagctgt tctcgatccg gtggggcagg aggtccgtag acttcacgaa tcgtatctca
     3301 tgagcaaggg cgatcttctc gagatggagg gtgtcattca agacgctctc ggtggtggcc
     3361 agtacaacat tcgggtcgac caaggtggag cgatcgtgcg cgctcagctt tctggacgca
     3421 tgcgacgtca tcacattcgc gtgctgcccg gtgaccgcgt gcgcgtggcg gtgtcgccat
     3481 acgatctctc gcacggtctc atcgtgtacc gcggcaagtg agcccgagcc gcctctgggc
     3541 ggcgtcgctg ttgctcgctt gcctcgcggc gtgctctcgc ggtgagcgcg aaggttcggg
     3601 tggctccgcg tcgagcgagc cgccctctcc gccattgccg gtggcggcgg aggtgccgag
     3661 cgctccgctc gctctgctac acgtgccgag cgcggacgag gctctgcctc ctcctattgg
     3721 aggtggactc ttgcccggcg cacctcctgc ggcgcgttcg cgctgccctc ccgaaatggt
     3781 cgatatttct gggcagttct gcatcgatcg ctacgagagc agctacgtcg actccgcgag
     3841 cggtctcgcc ctgtcgccgc actaccatcc gacgcgcgca cagacgagcg cgagctacga
     3901 gcgttaccgt cgtcggtcat cgaacgattc gctcgcagtc cccgagccgc ctccgtttca
     3961 actcgaacga gaccccgagc ccgaagcgcg atcgttgccg ggtgtcatcc ctcaaggcta
     4021 cgtgagccaa gagatcgccg agcgtgcttg ccgagccgcc ggcaagcgcc tgtgctcgct
     4081 cgccgaatgg gtacgagctt gccgcggcga gcagaaccga aagtacccgt acggcgagca
     4141 atatcgcgaa ggtgtgtgta acgttttccg ggaggctcat ccggcggcca ggctgcacgg
     4201 tgatgctagc cgcgagcacc tcgatcctcg gctcggcctc gttcaaggcg agcgcggtcc
     4261 gttgttgcga cgcacgggcg agacgacgga gtgtcgaagc gtctggggca acgacggcgt
     4321 gttcgatatg gtcggcaacc tcgacgaatg ggtcgccgac ggcggcttcg ctggcggctt
     4381 cttttcgcga ggcactcggg agggatgcga cgcgcggatc accacccacc cgggctacta
     4441 tttcgattac agcctgggcg gacgttgttg ccgctgaatg gcgagcgccc cagtatcccg
     4501 acgaaaaaaa tcgatgtagg tttttgtgcg cgcaggtggg tgcattcggt acgataggcc
     4561 ccatgaaatc gcgcgcattc tcccccgaac cctacgcccg cctcgtcgtg gcttcggttc
     4621 tgaccctctt cagcgccggc tgcggccatc cagtgcagcg ccagctggaa ggtacctggc
     4681 acggagacgc cgtcgagaac ttcgaagacc gagagctcgc tcgcgccacg ggatgggcca
     4741 agagccttcg gttcactttc agcggctcca aaatgagcgt ctcgatcgcc gccgaagaag
     4801 aacgaagcgg caactaccgc gtgcgctccg tgcacaactc ggacgtagtc ctcgccgtga
     4861 agaagcccga cggtgccgaa gacgtcgctc gcctcaagct cgacgacgac aactccattc
     4921 gctggatgtt gggcgattcg cgctcggtcg tactgcgccg cgagctttga gctccgcggc
     4981 gcggcgctca gcgggtgagc tctaggacgc gcgtgacact cgcgccttcg atcgacggcg
     5041 cgaaccggag ctcggccaag ccgtgctcgg tgcactcctg cacgctcggc gagagcggcg
     5101 gatcgaagct tacctgctcg atgctgccgt ctggcgcaac gctcaacgtg acggcgctct
     5161 gagccgtgac cttgagggtc cgcgagtgcg gcgtactctc gcggaagcac gtcgtgatgg
     5221 cgtcgacgac ttgagcgacg ccctgttcca cttcgccaat gttgagacgt cgcggtaccg
     5281 gtgccggctc tcggctcgtg gcgagaggca gcgccggttc ggcgggcgta gtctcgacgg
     5341 actcgacgcg cggagaaacc gacggtggag cgctgggcgt ccaacggcgc aatggtcggt
     5401 tggacgcgaa cggcgggctc tgcttcgcga ccgcgaccgg cgcctgatcc aaggggagac
     5461 cactca
//
LOCUS       DASTKS010000943         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_597007, whole genome shotgun sequence.
ACCESSION   DASTKS010000943 DASTKS010000000
VERSION     DASTKS010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597007"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFQ35_19825"
     CDS             <1..791
                     /locus_tag="VFQ35_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932967.1"
                     /translation="QKRDALHVFPLYYSLWGKNERHTTVFPLFHYGYKDNSWKFANPL
                     FFMSKGEEGESTFVTWGYARYRGRTELDMITPLFWHYRDPDIGLDQKILFPFYYSRTS
                     ARESSHAVFPFFGQFERFGISKSTWITPLFQHSRDLRGWSTNLYPLVYIGRDAAETHT
                     VVAPFFWDFASPKGRSTVAFPLYFRFSDDRDVSQLVGNVYYHETKVAGGLDWQIHIFP
                     AFSYGETPDGHWWNVLFGLAGYTRRARMTQIRTLWIPITLSGGE"
     gene            791..1936
                     /locus_tag="VFQ35_19830"
     CDS             791..1936
                     /locus_tag="VFQ35_19830"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET9932968.1"
                     /translation="MPAVPSSLRLANQATLLEQLLERKAASRAELAKATGMSQPTAGK
                     IIDDLVHAGVVEEIKSAEVGRQSVGRPGKQLRLSTSRPRFVVMELGVEWTRISALPPS
                     PPDIERWDVQFKTPNSEAAWQEKLFQNAERIDVKRPWAVLVAAPGIIDEKAGRVLLSP
                     NLHWSEHADLQWMLRQVWPAPVGLIQEVRAAALGELGTRPDSDDFLMVDIAEGIGGAL
                     MLARRPYQGALPMSGEIGHTPIPGNTRPCGCGGRGCLETLASERGMVQSLREVTKNPN
                     ATFADVVKATEKELPQWLRSTFDAVAMCIAAALNLYGVRRAVLVGRVTELPTIATDYL
                     VGAVQRASMWSRFDAVAVSLAPKRRARGLVVHGIHRFVMPSDWSRKR"
     gene            1933..2982
                     /gene="argC"
                     /locus_tag="VFQ35_19835"
     CDS             1933..2982
                     /gene="argC"
                     /locus_tag="VFQ35_19835"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9932969.1"
                     /translation="MKRFRAAVLGGSGFAGAELVRRLLLHPDVELVRVGAADHIGEPL
                     SSALPHLEGQTALRFENPSPEQAVEGVDIVLMGLPQKASIPVVEAALTSGAKVIDMSG
                     AFRLRSAEQYARYYGEVHPRPDLLARFVYGLPEQNRDAIRQSPFVASPGCFATTIELG
                     LLPFARAGLLDGPVHSVGITGSSGSGVTPSPTTHHPTRAGNLRTYRPLVHQHTPEVED
                     ALTVAGARRFRLDFVPVSAPLVRGILVTSFVEVPAALDDGALKDIVRKAYARERFVRV
                     PAARLPEVVAVAGSNFAEVGFVAGQVEGDKRTLTVFSALDNLVKGGAGQAIQNMNLML
                     GLSEATALEDVGAYP"
     gene            complement(3030..3854)
                     /locus_tag="VFQ35_19840"
     CDS             complement(3030..3854)
                     /locus_tag="VFQ35_19840"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9932970.1"
                     /translation="MATALGRAPALGVAFALLASTAGCDRAESQAHAAPREQAVSPIA
                     TQDAQLAAVSKRRVAPVDNRCPADMVLVEGDYCPKVELNCKKYQDPEGRYEHFRCAEY
                     GPSTCKSKQRKHLRYCIDKHEYVPPGESLPANHKSFTHAEKTCKAQGKRVCMESEWNF
                     ACEGEEMRPYPYGFSRDPGACNADREDIIATNGELRDLRAPPGSHPRCASPFGVLDLS
                     GNLEEFVAIDGSTPVRPAMKGAYWQPGRNFCRAAQTAHDRYYNGTETGFRCCSESK"
     gene            complement(4277..>4677)
                     /locus_tag="VFQ35_19845"
     CDS             complement(4277..>4677)
                     /locus_tag="VFQ35_19845"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9932971.1"
                     /translation="GKRVCVESEWNFACEGEEMLPYPYGWTRKDVCNQDRLDLYEPNP
                     KRQVLRDHRRASGSLAECKSPFGVFDMVGNMDEPVLREAQRYAYPFRNGLKGGWWMAG
                     RNRCRPATTAHDDHYRDIQIGIRCCRDALQ"
BASE COUNT          815 a         1528 c         1467 g          867 t
ORIGIN      
        1 cccagaagcg cgacgcgctc cacgtatttc cgctctatta cagcctgtgg ggcaagaacg
       61 agcggcacac caccgtcttt ccactctttc attacggtta caaagacaac tcgtggaagt
      121 tcgcgaaccc gctcttcttc atgagcaagg gcgaagaggg cgagagcacg ttcgtgacgt
      181 ggggctatgc gcgctatcgc ggccgcaccg agctcgacat gatcacgccg ctcttctggc
      241 actatcgaga tccggacatc ggcctcgatc agaagatcct ttttccgttc tattactccc
      301 gcacgagcgc tcgtgagtcg agccacgcgg tgttcccctt cttcggccag ttcgagcgct
      361 tcgggatcag caagtcgacc tggataacgc cgctgttcca gcactcgcgc gatctgcgcg
      421 gctggtcgac gaacctctac ccgctcgtgt acatcggtcg cgacgcggcc gagacgcaca
      481 ccgtggtcgc gccgttcttc tgggacttcg cctcgccgaa gggccgttcg acggtggcgt
      541 tccccctcta cttccgcttc agcgacgacc gcgacgtgtc gcagctggtc ggaaacgttt
      601 actatcacga gaccaaagtc gcaggcggcc tcgactggca gattcacatc tttcccgcgt
      661 tttcctacgg cgaaacgcct gatggtcatt ggtggaacgt tttgttcggg ctcgcgggtt
      721 acactcgccg agcgcgcatg actcagattc gaactctctg gatcccgatc acgctgagcg
      781 gaggcgaata atgcccgcgg tgccgtcgag cttacggctc gccaaccagg cgaccttgct
      841 cgaacagctc ctcgagcgga aagctgcgag ccgcgcggag cttgcgaagg caaccgggat
      901 gagccagccg acggccggca agatcatcga cgacctcgtg cacgccggtg tagtcgaaga
      961 gatcaagagc gccgaggtcg gccgccaaag tgtgggaaga cccggaaagc aactccgctt
     1021 gtccacgtca cgcccacgct tcgtggtgat ggagctcggc gtcgagtgga cccgcatttc
     1081 ggcgctgccc ccgtccccgc ccgatatcga gcgctgggac gtccagttca agacgccgaa
     1141 cagcgaagcc gcttggcaag agaagctctt tcaaaacgcc gagcgcatcg acgtgaagcg
     1201 cccctgggcg gtgctcgtgg ctgccccggg catcatcgac gaaaaggccg ggcgcgtttt
     1261 gctatcgccg aacttgcact ggtcggaaca cgccgatttg cagtggatgc tgcgtcaggt
     1321 gtggccggca ccggtggggc tcatacaaga ggtgcgcgcc gccgcgctcg gtgagctcgg
     1381 tactcgaccg gacagcgacg acttcttgat ggtggatatc gccgaaggga tcggcggcgc
     1441 gctgatgctg gcgcgtcgtc cgtatcaagg cgcactgccg atgagcggcg aaattggcca
     1501 caccccgatt ccgggcaaca cgcgtccgtg cggctgcggc ggccgcggct gcctcgaaac
     1561 gctcgcgtcg gaacgcggta tggttcagag cttgcgcgag gtcaccaaga accccaacgc
     1621 gacgttcgcg gacgtcgtca aagcgaccga gaaagagctg ccgcagtggc ttcgttcgac
     1681 gttcgacgcg gtcgcgatgt gcatcgcggc ggcgctcaac ttgtacggag tgcgccgcgc
     1741 ggtgctcgtg gggcgagtca ccgagttacc cacgatcgcc accgactacc tggttggcgc
     1801 cgtgcagcgc gcgtccatgt ggagccgttt cgacgccgtc gcggtgagcc tcgcgcccaa
     1861 gcgacgtgca cgcgggctcg tggtgcacgg catccatcgc ttcgtgatgc cgtcggactg
     1921 gtcgcgtaaa cgatgaaacg atttcgagca gcggttctcg gcggctctgg gttcgccggc
     1981 gccgagctcg tgcgtcgctt gctgttgcac cccgacgtag agctcgtgcg cgtcggcgcg
     2041 gccgatcaca tcggtgagcc gctctcgagc gcgctgcccc acctcgaggg tcaaacggcg
     2101 cttcgcttcg agaacccgag ccccgagcaa gccgtcgaag gcgtcgacat cgtgctcatg
     2161 ggcttgccgc agaaagcgag catccccgtc gtcgaagccg ccctcacctc cggcgcgaag
     2221 gtcatcgaca tgtcgggcgc cttccggttg cgcagcgccg aacagtacgc gcgctactac
     2281 ggcgaagttc acccccgtcc ggatctcttg gctcgcttcg tttacggttt gccggagcag
     2341 aaccgcgacg ccatccggca aagcccgttc gtagcgtctc ctgggtgctt cgcgaccacg
     2401 atcgagctcg ggttactgcc cttcgcccgc gccgggttgc tcgacggccc cgtgcattcg
     2461 gtgggcatca ccggctcgag cggcagcggc gtgactcctt cgccgaccac gcaccatcca
     2521 acgcgcgccg ggaacctgcg aacgtatcgt ccgctcgttc accaacacac gccggaagtc
     2581 gaagacgcgc tcacggtcgc aggggcgcgg cggttccgct tggacttcgt tcccgtctcg
     2641 gcgccgctcg tgcgcggcat cttggtcacg agcttcgtcg aagtcccggc cgccctcgac
     2701 gacggcgcgc tgaaagacat cgtccgaaag gcatacgcca gggagcgctt cgtgcgcgtc
     2761 cccgccgctc gcttgcccga agtcgtcgcg gtcgcaggct cgaacttcgc cgaagtcggc
     2821 ttcgtcgcgg ggcaagtcga aggcgacaag cgaacgctga ccgtgttctc ggcgctagac
     2881 aacctcgtga agggcggcgc cgggcaagcg attcagaaca tgaatctgat gctcggcttg
     2941 tccgaggcta ccgcgctcga agacgtcggc gcgtacccgt aaaagagcac gtcgccgttc
     3001 gcggtcgcgt ggaacccgcc tcgcggcgcc tacttggatt ccgagcagca gcggaacccg
     3061 gtctcggtgc cgttgtagta gcgatcgtgc gccgtctgag cggcgcgaca gaagttccgc
     3121 cccggttgcc agtaagcgcc cttcatcgcg ggacgaaccg gcgtgctccc gtcgatcgcc
     3181 acgaactctt ccaggttccc cgacaaatcg aggaccccaa acgggctcgc gcaacgcggg
     3241 tgcgagcctg gaggcgcccg caaatcacgg agctcgccgt tggtggcgat gatgtcttca
     3301 cggtcggcgt tgcacgcgcc cgggtcgcgc gagaagccgt agggatacgg gcgcatctcc
     3361 tcgccttcac aagcgaagtt ccactcgctt tccatgcaca cgcgctttcc ctgcgctttg
     3421 caggtcttct cggcgtgcgt gaagctcttg tggttcgccg gtaacgactc tccgggcgga
     3481 acgtactcgt gcttatcgat gcagtaacgt aagtgcttac gttgcttcga cttgcaggtc
     3541 gacggaccgt attcggcgca gcggaagtgt tcgtagcgcc cttccggatc ttggtacttc
     3601 ttgcagttga gttcgacctt gggacagtag tcgccttcga cgaggaccat gtccgccggg
     3661 cagcgattgt cgaccggtgc gacgcggcgc ttgctcacgg ccgcgagctg cgcgtcttgc
     3721 gtcgcgatcg ggctcaccgc ctgttcgcgc ggcgccgcgt gggcctggct ctctgcgcgg
     3781 tcgcagccag ccgtcgacgc gagcagcgcg aacgcgactc cgagagctgg agcgcgaccc
     3841 agggcggtag ccatggggcg gcgggcggtc gggcaaacgt ctcggagtga agaggcggct
     3901 cggctacgca ctggacctcc gttttgcgta tttgcgtacg cagcgtcaag gcaattcctc
     3961 cgctcagccg gacgatgtca tcgaacaccg cactcgtcat actcatgatc acagccgaat
     4021 ttaacgtttc agatcattga attagacgcc aatcgcaaat gtgatggatc actttagatc
     4081 tcactatttc ataaaattcc acgagagaac gggtggccgg ctcgcgcatt ggacgacact
     4141 cacggacttg tgagcaagcg cgagcgccgg tgcggaaagc cgcgacactc gaagcaactc
     4201 acacaatcgc gagcaccgcg gcgaaagcgc tccgtcacat gcgtcttcgc ccacgccagc
     4261 gcgcgtccgt gatcgcttat tgaagcgcgt cgcgacaaca gcgaatgcca atttggatgt
     4321 cgcgatagtg gtcgtcgtgc gcagtagtcg cgggtcgaca acggttacgt ccggccatcc
     4381 accagccgcc cttgagcccg ttgcggaacg gatacgcata ccgctgggct tcgcggagca
     4441 ccggctcatc catgttgccg accatgtcga acacgccgaa cgggctcttg cactcggcga
     4501 gagagcctga cgcccggcga tgatcgcgca gcacctggcg cttgggattc ggttcgtaca
     4561 ggtccaagcg atcttggttg cagacgtcct ttcgcgtcca accgtagggg tacgggagca
     4621 tctcttcgcc ttcacacgcg aagttccact cactttcgac gcagacgcgc ttaccca
//
LOCUS       DASTKS010000944         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1492306, whole genome shotgun sequence.
ACCESSION   DASTKS010000944 DASTKS010000000
VERSION     DASTKS010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492306"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFQ35_19850"
     CDS             complement(<1..432)
                     /locus_tag="VFQ35_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932972.1"
                     /translation="MSRYLPLQDATVFAYDTTSGAEAQKGMLVLEIRRPRTGMAELIV
                     AGRTRRVEIDDTGIHHATGGYLLKPPLTLHAKFPGDFGMVEITAIDKVVTVPAGSFAG
                     CIETAEELTNAEFSKRTVTTYCPDVGIVARRTEAETNEGNLS"
     gene            complement(533..1078)
                     /locus_tag="VFQ35_19855"
     CDS             complement(533..1078)
                     /locus_tag="VFQ35_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932973.1"
                     /translation="MSDEDQNREATATDANEASANAPQGSTDASEASASGTEGSTDAN
                     EASASGSEGPTGAVESGVAAHEARADRPKKKRKKRRAEPVERDELDAAGMQRPAFVLD
                     FPRDPELDVLVRAFEAGNFAFVREQAPRLAESSLDADVKAAARELARRIEPDPLVKFL
                     LGVAVALFVVVVTYVYHSHGR"
     gene            complement(1078..>2269)
                     /locus_tag="VFQ35_19860"
     CDS             complement(1078..>2269)
                     /locus_tag="VFQ35_19860"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HET9932974.1"
                     /translation="LLWLTVVLYLGLLGFFKYYDFGVESLAAALGYLGYHPPEWALHI
                     TLPVGISFFTFESMSYVIDVYRGDIEPHSSYLEYLTFVAFFPHLVAGPIVRPRDLLPQ
                     LAAPPRFDSVLGAEGLFLIAVGLLKKVAIGDYLALNLVDRVFDAPRSYSSLECYLGVT
                     GYALQIYSDFSGYTDIAIGSALLLGVRFPKNFDAPYTAVSIVDFWRRWHISLSTWLRD
                     YLYISLGGNRRGRARTYLNLVLTMLLGGLWHGASWTFVAWGALHGAALALTRALTSPF
                     AALTRKWPDGVKRALGIAFTFHLVCLGWILFRSDSFGKALLVLQRLGEGSLYHPNVDH
                     RVLLVMAAGFFGHFFPARWFSALRDGFCRLPALGQAALLFGVLLLVRRMASAEAVPFV
                     YFQF"
BASE COUNT          441 a          711 c          757 g          360 t
ORIGIN      
        1 gctcaagttg ccttcgttcg tctcggcttc ggtacggcgc gctacaatcc cgacgtcagg
       61 gcaatacgtc gtgacggtgc gcttgctgaa ctctgcgttc gtgagctctt cggccgtttc
      121 gatgcagcct gcgaagctac cggcgggtac cgtgaccacc ttgtcgatcg cggtgatctc
      181 gaccatgccg aagtcgcccg ggaactttgc gtgaagcgtc agcggaggct tcaaaaggta
      241 tccgcccgtc gcgtggtgaa tcccggtgtc gtcgatttct actcgacgcg tgcgcccagc
      301 gacgatgagc tctgccatgc ccgtgcgcgg tcgcctgatt tcgaggacga gcataccctt
      361 ttgtgcttcg gctcccgacg tagtgtcgta cgcaaagacg gtcgcgtctt gcagcggtag
      421 gtaacgagac acttcgcttg cagctcgcgg cggcggaggg gcggcaggcg gaggcgcggt
      481 cgctttcgag caactggcga gggccacgac acaagccaga acgaagcgcg tctcagcggc
      541 catgcgagtg ataaacgtag gtgacgacga cgacgaacag cgccaccgcg acgcccagca
      601 gaaacttcac cagcgggtcc ggctcgatgc gccgagcgag ctcgcgagcc gcggctttca
      661 cgtccgcgtc caggctcgac tccgcgagac gcggcgcttg ctcgcggacg aacgcgaagt
      721 tgccggcctc gaaagcgcgg accaagacgt cgagctcggg gtcgcgcggg aaatcgagca
      781 cgaaagcggg ccgctgcatt cccgccgcgt ccagctcgtc gcgctcgacg ggttcggcgc
      841 ggcgcttctt gcgcttcttc ttcggtcgat ctgcccgcgc ttcgtgcgcc gcgacgccgg
      901 actcgaccgc gccggtcgga ccttcgctgc cgctcgccga cgcttcgttc gcgtccgtcg
      961 aaccttcggt gccgctcgcc gacgcttcgc tcgcgtccgt cgaaccttgc ggcgcgttcg
     1021 ccgacgcttc gttcgcgtcc gtcgccgtgg cttctcggtt ttggtcttcg tcgctcatta
     1081 gaactggaag tagacgaacg gtacggcctc ggcgctcgcc atgcggcgca cgagcagcag
     1141 cactccaaat agcagagccg cctggcctag ggcaggcaaa cggcagaacc cgtcacgcaa
     1201 cgcagaaaac cagcgcgcgg gaaagaaatg gccgaagaag ccggcggcca tcaccaagag
     1261 cacgcgatgg tcgacgttcg ggtggtagag gctgccctcc cccaggcgct ggagcacgag
     1321 gagcgccttt ccgaacgagt cggagcgaaa caggatccat ccgaggcaaa ccaaatgaaa
     1381 tgtgaaggcg atgccgagcg cccgcttaac gccgtctggc catttacgcg tcagtgcggc
     1441 aaacggcgac gtcagcgcgc gggtgagcgc gagcgcagcg ccgtgcagcg caccccaagc
     1501 aacgaaggtc caactcgctc cgtgccaaag cccgccgagc agcatcgtga gcaccaagtt
     1561 caggtacgtg cgggcgcgac cgcggcggtt accgccgagc gagatgtaca ggtagtcgcg
     1621 tagccacgtg gagagcgaga tgtgccaacg ccgccagaag tcgacgatgc tgaccgcagt
     1681 gtatggcgcg tcgaagttct ttggaaagcg cacgccgagc agcagcgccg agccgatcgc
     1741 gatgtcggtg tacccggaga agtccgaata gatttgtagc gcgtagccgg taacgcccaa
     1801 gtagcactcg agcgaggagt agctgcgcgg agcgtcgaac actcgatcga cgagattcag
     1861 agccaaatag tcgccgattg cgaccttctt caggagcccc accgcgatca agaacagccc
     1921 ttcggcgccg agaacggagt cgaagcgcgg gggcgcggcg agctgaggca agaggtcgcg
     1981 cggtcgcacg atgggcccgg ccacgaggtg cgggaagaac gcgacgaacg tgaggtactc
     2041 gaggtagctc gagtgtggct cgatgtcgcc acggtagacg tcgatgacgt agctcatcga
     2101 ctcgaaggtg aagaagctga tgccgaccgg cagcgtgatg tggagcgccc actccggcgg
     2161 gtgatatccg aggtagccga gcgccgccgc gagcgactcg acgccgaaat cgtagtactt
     2221 gaagaagccg agcaggccga ggtacaagac cacggtgagc cacaaaagc
//
LOCUS       DASTKS010000945        10416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_373872, whole genome shotgun sequence.
ACCESSION   DASTKS010000945 DASTKS010000000
VERSION     DASTKS010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373872"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFQ35_19865"
     CDS             complement(<1..436)
                     /locus_tag="VFQ35_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932975.1"
                     /translation="MSAPDSSLLTKVVRHLSTPPAYAESGVRGSILTAAATSYGFRAD
                     MDELTQPTGFDPEAAQLFEALVESAFLVANADGHFDDKEQKAFQHVVLAACGGFVLER
                     QVRALLSDLSDSLAEDGIEKRIDAIARAVTKPEHAREVLRVAA"
     gene            complement(611..1645)
                     /gene="lipA"
                     /locus_tag="VFQ35_19870"
     CDS             complement(611..1645)
                     /gene="lipA"
                     /locus_tag="VFQ35_19870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9932976.1"
                     /translation="MESEAPEKRPAPRDPKPPWLKVRAPGGEKYTQLKETLRGLDLYT
                     VCEEARCPNVGECWGAGTATVMLLGHTCTRGCRFCAVTTGNPRGAFDPREPEHVARAI
                     AKLGLRYVVLTMVDRDDLLDGGAEQVGRTVSALAREVPELLVETLVGDFGGRLHDVER
                     VLDSGPAVFAHNIEVVRRLTPLIRDQRCSYDRSLDVLRHAKSRHPERLVKSSIMVGIG
                     ETDEEVEATLVDLRGAGVDIVTLGQYLRPTPKHAAVDRYVTPATFERFREQAEKLGFA
                     FAASGPLVRSSYHAAEGFVAARLRPDAPPIPPRPISPTRAVDGPVDPGLIGIDSLLRR
                     RKPAESREEG"
     gene            complement(1735..3156)
                     /gene="gatB"
                     /locus_tag="VFQ35_19875"
     CDS             complement(1735..3156)
                     /gene="gatB"
                     /locus_tag="VFQ35_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET9932977.1"
                     /translation="MEYEPVIGLEVHAQLLTQSKLFCSCSTHFGGSPNSNVCPVCLGL
                     PGSLPVLNARAVEMAVSAALAVGCSIRGTSIFARKNYFYPDLPKGYQISQYEEPFSDN
                     GFLEIEVDGQRRRVGITRVHMEEDAGKNVHGVGGDSLVDLNRAGTPLIEIVGAPDLRS
                     SAEAAAYLRALRDVLMFVGVNDGNLEEGSFRCDANVSIRPRGSTQFGTRCELKNINSF
                     RFVQRAIDVEIARQTAILDAGGKVTQETRSFDPETSQTKTLRSKEDAHDYRYFPEPDL
                     PPLKLSSAFVEKLRTELPELPEAARARLVAAGIPAATAQTIVQHPKYLRFFDELAQKT
                     SPVRAANFFVNEVLRGAKVHGLDGSFTVSATQVAELLNLVEKGDISGKQAKDVYAAVE
                     GTDKSPSAVVKEQGIVVVSDEGTLLAVCEAVVKANPGQAAAFRSGKKGLLGFFVGQVM
                     KETKGSGNPKLVSELLERILGSS"
     gene            complement(3174..3842)
                     /locus_tag="VFQ35_19880"
     CDS             complement(3174..3842)
                     /locus_tag="VFQ35_19880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET9932978.1"
                     /translation="MADLDSTSVAPLGPRQVTGKTVSRPKRGAGVQTYVPEIAKGLLI
                     TMKHFFQNTKEMALGMRNDPTLEAIEDGVNTVSYPEQKRPYAARFRGVHRLTHRDDGS
                     PRCVACLCCSTACPAQCIHIVAGEYPPGDPRRGYERFPQKFVIDELRCVFCGFCVEAC
                     PCDAIRMDTGMHAAPYDSREQFLFDKDLLMGFAGRDGSQATANPRHEPGEPSYPGVSR
                     EKGH"
     gene            complement(3847..5643)
                     /locus_tag="VFQ35_19885"
     CDS             complement(3847..5643)
                     /locus_tag="VFQ35_19885"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9932979.1"
                     /translation="MNIPLPELGIFLLRAFVVVMFAMNVAVLLTWADRRQGAAIQDRV
                     GPNRAVIWIPTPIAQALALLPALGLAGGVLLLLAKHDFEGPMKPFAAAAFSHLAILWV
                     WLTGLIIAGLVAARGARNSFDVFIASIGNPRRFFYAGFAAHLILFAATLMYRGTEQGA
                     LLEQLGLSAFPAMFAFAVVAGAGYAAFSIRKEPRVGLRLVGLLHPAADGLKTIFKEDF
                     IPPNADKFLHSIAPIIAFFPALVVLGVVPFGSALCFGTNPETGRIAFDKLYDVLPAAG
                     CTGEGMKQLPLQVVGLDVGVLYFFALAGTGIVGAALAGWASDNKYSLLGGLRAASQMV
                     SYEVTMGLTLVGVFMLYGTLRVDEMVRWQAENTWGIFLQPVAFVLFFAAAVAESKRIP
                     FDIPEGESEIVAGYYTEYAGMKFAMFFFAEYIAVVTSSALMAALFLGGWDLPFIRHDG
                     LHIAIGQSVFVHEELPHVLVVVLGALGFVLKTVTLCWLQLTIRWTLPRFRYDQLMRLG
                     WRKLLPASLVNILVTGVLILAVQGAGESVLHGLETAAAVTKLLIAVAGIAGVVALVLF
                     LLRPVKKRRMLATTSAQFGATLGGTQQARMEA"
     gene            complement(5640..7238)
                     /locus_tag="VFQ35_19890"
     CDS             complement(5640..7238)
                     /locus_tag="VFQ35_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9932980.1"
                     /translation="MPTFKLDDREIPFEQGDTIIRAAHRVGIDIPHYCWHPGLSVAAN
                     CRMCLVEVMPPPGRPAMMLDVLEWDAKKGDYVPQKKPKLQPACQFAAADGLVVKSQTS
                     EHVTKARSAVQELLLLNHPVDCPICDQAGECRLQDYWLEHQGQKKRMRDEPVHKPKAV
                     VFGPTIVYDAERCIVCTRCVRVTAELANDPVLSVRERGNLNEITVSPGRQLDNKYTLM
                     TEYVCPVGALTSKDFRFKARVWFLRSARTVCTGCATGCNSFTDFEPREQKVYRYRPRE
                     NLAVNKYWMCDDGMLDYRRIHENRVLSARLRGEASTREAAVEKAAELLRGKDAASVAV
                     VLSAEHSQEDNLALIWLAREVLGLSQVFVTGRPEGYSDDILINPDKNANSAGVAELVD
                     TIKLRSFSEFESAVSGGKLDVVVSLGSEVPHKTDAVSKIRSLIALSMHEGPWAKAAAV
                     LLPASTWAEADGTFVNAKGLAQVSEKAIGSQGDSKPAWRLVSELAKALGRAPGWRRSE
                     DLLNAVTKTARSSAPPAPTAGANG"
     gene            complement(7276..8490)
                     /locus_tag="VFQ35_19895"
     CDS             complement(7276..8490)
                     /locus_tag="VFQ35_19895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9932981.1"
                     /translation="MEPLDQELEESELELPAEPMLLNVGPAHPAMHGTVRCVMELSGE
                     VIERVDVQVGYLHRGFEKMCERGTWTQVFPYVDRTNYVSPMLCNVGFALACEKLLDVK
                     VPDRCQWYRMALGELARISDHLTCDGAMAMELGAFTPFLWMIKGRDMIWDLMEEETGA
                     RMTHSFGRVGGMAAPPTDGFKENARAVVRQILHILEEVEKLLLGNRIFLDRLEGVGVI
                     SQQDAISLGWTGPCLRATGIPYDVRKAHPYLKYDEVEFEVPVGSASDCFDRFMVRIEE
                     IKQSALIIEQCLQRMPDSGPINIDDPRIVLPPKSEVYTTIEGTIQHFKIVMEGIKLPA
                     GEAYGYIEGGNGEYGFYLVSDGSGTPYRVRIRPPCYYVTGGLERVITGQMMADVVPCF
                     GSLNMIGGECDR"
     gene            complement(8490..9086)
                     /locus_tag="VFQ35_19900"
     CDS             complement(8490..9086)
                     /locus_tag="VFQ35_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9932982.1"
                     /translation="MAKVLIDLVRQKFPNAVIETHAAHGDDTIVLEAASWHAVAQFLR
                     DDPRANMQMLVDLTVVDYPDRDPRFEVVAHLLSLSKGHRVRIKTRVGDPEGDSARVDT
                     LADLWGSANWAAREAYDMFGVVFVGHPDLRRILMYPEFEGYPLRKDYPAEKIQPLVEY
                     RDVPNIEKLPPFGPDEGMSFGRQTHARGRVAPDEVESN"
     gene            complement(9112..9702)
                     /gene="nuoB"
                     /locus_tag="VFQ35_19905"
     CDS             complement(9112..9702)
                     /gene="nuoB"
                     /locus_tag="VFQ35_19905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET9932983.1"
                     /translation="MGELKQTTTHIIEPGGEQGFATTRFESILAWAQKYSLFMYPFVT
                     ACCGMEFMAVSSPRYDFSRFGSEAPRFSPRQSDLLWVVGTITQRQAPILRRIYEQMAE
                     PKWVLAFGTCASCGGFYDNYTTVAGIDKIIPCDVYVPGCPPRPEAVLDGLMLLQDKIQ
                     SGDRRPAIVKPRHDPVQDPNMGLVQLRRSRPSRPPT"
     gene            complement(9690..10049)
                     /gene="ndhC"
                     /locus_tag="VFQ35_19910"
     CDS             complement(9690..10049)
                     /gene="ndhC"
                     /locus_tag="VFQ35_19910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9932984.1"
                     /translation="MVELYLPMFLMFLIVLVIASVMFLGGQFIGPKNPTPEKMTPFEC
                     GNDTEGARGVRMSVKFYLTAILFVVFDVETVFMYPWAALFRSLGWTGFLSMFGFIVAL
                     LVALAYCWKKGALEWES"
     gene            complement(10182..>10416)
                     /locus_tag="VFQ35_19915"
     CDS             complement(10182..>10416)
                     /locus_tag="VFQ35_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932985.1"
                     /translation="GPLRRAANLGAEGDRVWPLLARALMERGRLLAAYAAVLEGQSAG
                     VSADALESVLQHVSQGLGEPFQALRQSIEKTAS"
BASE COUNT         1863 a         3373 c         3435 g         1745 t
ORIGIN      
        1 gtgccgcaac gcgcaaaacc tcgcgcgcat gctccggctt ggtgacggcg cgcgcaatcg
       61 cgtcgatgcg cttttcgatt ccgtcctctg cgagcgagtc ggagaggtcg gacaggagcg
      121 ctcgcacctg ccgttcgagc acgaagcccc cgcacgcagc gagcacgacg tgctgaaaag
      181 ccttctgctc cttgtcgtcg aagtggccgt cggcgttggc gaccaaaaac gcgctctcga
      241 ctagcgcttc gaacagctga gcggcctccg ggtcgaaacc ggtgggctgc gtcaactcgt
      301 ccatgtcggc gcggaagccg taagacgtgg cggcggcggt gaggatcgag ccgcggacgc
      361 cgctctcggc gtaagcgggc ggcgtgctga ggtggcggac caccttggtg agcagactcg
      421 agtccggtgc agacatgttc ctcggcagag aggaccacta ttacatcaga accgcctcac
      481 ccaaatgtgg ttttgctgtt accggaacgc cggcgcgccg ccgcagcagc gagattctcc
      541 agcgaagtcc ggggagaagg acctcgcggc gcaccacggc tcggtggttc cccccgatat
      601 tcgcggactt ctaaccctct tcccgagatt ctgccggttt tcgccgcctt aggagcgaat
      661 cgataccaat caagcctggg tcgaccggcc cgtctaccgc tcgcgttggc gagatgggcc
      721 gcgggggaat tggcggagcg tcgggccgca agcgcgccgc aacgaagccc tcggccgcgt
      781 gatagctaga gcgaacgagc ggacccgacg cagcgaacgc gaagccgagc ttctctgctt
      841 gctcgcgaaa gcgttcgaac gttgcgggcg tgacgtatcg atcgaccgcc gcgtgcttcg
      901 gcgtcgggcg caagtattgg ccgagcgtca cgatgtcgac ccccgcgccg cgcagatcga
      961 cgagcgtcgc ctcgacttcc tcgtcggttt cgccgatgcc gaccatgatc gagctcttca
     1021 cgaggcgctc tgggtgccgc gatttcgcgt gccgcaaaac gtcgagcgaa cgatcgtagc
     1081 tgcagcgctg atcgcggatg agcggcgtca ggcggcgcac cacctcgatg ttgtgcgcga
     1141 acacggcggg cccgctgtcg agcacgcgct cgacgtcgtg aagccgtccg ccgaaatctc
     1201 cgacgagggt ctcgacgagc agctccggca cctcgcgagc gagcgcactc acggtgcgcc
     1261 ccacctgctc ggcaccgcca tcgagcaagt cgtcgcggtc gaccatggta aggacgacgt
     1321 aacgcaggcc gagcttggcg atcgcgcgcg caacgtgctc cggctcgcgc ggatcgaaag
     1381 caccgcgcgg attgccggtc gtaaccgcgc agaagcggca gccgcgggtg cacgtgtgac
     1441 cgagcaacat cacggtcgcg gttcccgcgc cccaacactc gccgacgttc gggcagcgtg
     1501 cctcttcgca gaccgtgtag aggtcgagcc cgcgcagcgt ttccttgagc tgcgtgtact
     1561 tttcaccccc gggcgcgcgc accttcagcc acgggggctt ggggtcacgc ggcgcagggc
     1621 gcttttcggg agcttcgctc tccatggcgg cgaatctacg gctcgaagcg gcagcgcgcc
     1681 agcgccgtta ccgctgcgtc gcggcgaagc gccaccgggc gccctccgca gcgatcacga
     1741 actaccgagg attcgctcca agagctcgct cacgagcttc gggttgcccg aacccttcgt
     1801 ctctttcatc acttgtccga cgaagaagcc gagcaggccc ttcttgccgg agcggaaggc
     1861 cgccgcttga ccagggttcg ccttgacgac cgcttcgcat accgcgagca gcgtgccctc
     1921 gtccgaaacg acgacaatcc cctgctcttt gacgacggcg ctcggcgact tatcggtgcc
     1981 ctcgaccgca gcgtagacat ccttggcttg cttgccggag atgtcgccct tttcgaccaa
     2041 gttcaaaagc tcggcgactt gagtcgcgct cacggtgaag ctgccatcga ggccgtgcac
     2101 cttcgcgccg cggagcacct cgttcacgaa gaagttcgcg gcgcgaacgg ggctcgtctt
     2161 ctgcgcgagc tcgtcgaaga agcgaaggta cttcgggtgc tgaacgatgg tctgcgccgt
     2221 cgcagctggg atccccgcgg cgacgaggcg ggcgcgcgct gcctcgggga gctccggcag
     2281 ttcagtgcgg agcttttcca cgaaggcgct gctcagcttg agcggcggca aatccggctc
     2341 ggggaagtag cgatagtcgt gcgcgtcttc cttcgagcgc agcgtcttgg tttggctcgt
     2401 ctccgggtcg aagctacgcg tctcttgagt gaccttcccg cccgcatcca aaatcgcggt
     2461 ttgccgggca atctcgacgt cgatggcgcg ctgcacgaag cggaacgagt tgatgttctt
     2521 gagctcgcag cgcgtgccga actgggtcga gccgcgcggg cggatcgaaa cgttggcgtc
     2581 gcagcggaag ctcccctctt cgaggtttcc gtcgttcacg cccacgaaca tcagtacgtc
     2641 gcggagcgcg cgcaggtaag cggccgcctc cgcgctggag cgcaaatccg gagcgcccac
     2701 gatctcgatg agcggcgtgc cagcgcggtt caagtcgacg agcgagtcgc cgcccacgcc
     2761 gtggacgttt ttcccggcgt cctcttccat gtgcacgcgc gtgatcccga cgcggcggcg
     2821 ctgaccatct acctcgatct cgaggaagcc attatcggag aacggctcct cgtattggct
     2881 tatctggtag cccttcggca gatcgggata gaagtagttc tttcgggcga agatgctggt
     2941 gccgcggatg ctgcagccaa ccgcgagcgc cgcgctcacc gccatctcga cggcgcgcgc
     3001 gttgagcacg ggcagcgagc ccggcagtcc gaggcacacc gggcacacat tcgagttcgg
     3061 cgagccgccg aagtgcgtcg agcacgagca gaacagcttg ctctgtgtga gcagctgggc
     3121 gtgcacctcc aagccgatga cgggctcgta ctccatctag gctccctcga ggctcagtgg
     3181 cccttctcgc ggctgacgcc tgggtagctc ggctcgcccg gctcgtggcg cgggttcgcg
     3241 gtcgcttgcg aaccgtcgcg gccggcaaag cccatcagca agtctttatc gaacaggaat
     3301 tgctcgcgcg aatcgtacgg agcggcgtgc atgccagtgt ccatacggat ggcgtcgcag
     3361 ggacaagcct cgacgcagaa accgcagaac acgcagcgga gctcgtcgat cacgaacttc
     3421 tgcgggaagc gctcgtagcc gcgccgcgga tcgcccggcg gatactcacc ggcaacgatg
     3481 tgaatgcact gcgccgggca agccgtcgag cagcacagac acgccacgca acgcggcgag
     3541 ccgtcgtcgc ggtgggtgag gcggtgaaca ccgcggaaac gcgcggcgta gggccgcttc
     3601 tgctccgggt agctcaccgt gttcacgccg tcctcgatcg cctcgagggt tgggtcgttg
     3661 cgcatcccga gcgccatctc cttcgtgttc tggaagaagt gcttcatcgt gatgagcaag
     3721 cccttcgcga tctccggaac gtacgtctgc acgccggcgc cacgcttggg gcgcgacacc
     3781 gtctttccgg tcacctgacg cgggccgagc ggagcaactg aagtggagtc aaggtctgcc
     3841 atcgaatcac gcttccatac gagcttgctg ggtcccgccc agggtcgctc cgaattgcgc
     3901 cgaggtggtc gcgagcatgc ggcgcttctt cacgggccgc agcaagaaga gcaccaaggc
     3961 caccacaccg gcgatcccag cgaccgcgat gagcagcttc gtcaccgccg ccgccgtctc
     4021 gaggccgtgc agcaccgact cgcccgcccc ctgcactgcc aagatgagga cgccagtgac
     4081 gaggatgttg acgagtgaag ccggcaagag cttgcgccag ccgaggcgca tcagctgatc
     4141 gtagcggaag cgcggcaacg tccagcggat cgtcagctgt aaccagcaca gcgtgaccgt
     4201 cttgagaacg aagccgagcg cgccgagcac gaccacgagc acgtgcggca gctcctcgtg
     4261 cacgaacacg ctctgaccga tcgcgatgtg caggccgtca tgacggatga acgggaggtc
     4321 ccagccgccg aggaagagag ccgccatcaa ggcgctcgag gtgacgaccg cgatgtactc
     4381 ggcgaagaag aacatcgcga acttcatacc ggcgtactcg gtgtagtagc ccgcgacgat
     4441 ttcgctctca ccttcgggga tgtcgaacgg gatgcgcttc gactcggcaa ccgcggccgc
     4501 gaagaagagc acgaacgcga cgggctggag gaagatgccc caggtgttct cggcctgcca
     4561 gcgcaccatc tcgtcgacgc gcagggtgcc gtacagcatg aagacgccga cgagggtgag
     4621 gcccatcgtc acttcgtacg agaccatctg gcttgcagcg cgcaaaccgc cgagcaagct
     4681 gtacttgttg tcgctcgccc aaccggcgag cgcagcgccg acgatgccgg tgccggcgag
     4741 cgcgaagaag tagagcacgc cgacgtcgag gccgacgacc tggagcggca gctgcttcat
     4801 cccctcgccc gtgcagcccg cggcggggag cacgtcgtag agcttgtcga acgcgatgcg
     4861 gcccgtttcg gggttcgtcc cgaagcagag ggcgctaccg aacggcacga ccccgagcac
     4921 gaccagggcc gggaagaagg cgatgatggg cgcgatgcta tgaaggaact tgtccgcgtt
     4981 cggcgggatg aagtcctcct tgaagatcgt cttcaagccg tcggcagcgg ggtgcagcag
     5041 gccgacgagg cgcaagccga cgcgcggctc cttgcggatg ctgaacgccg cgtaacccgc
     5101 gccggccacg accgcgaacg cgaacatggc cgggaacgcc gagagaccga gctgctcgag
     5161 cagcgcaccc tgctccgtgc cgcggtacat gagcgtcgcc gcgaacagga ttaggtgagc
     5221 cgcaaacccg gcgtagaaga agcggcgggg gtttccgatc gacgcgatga agacgtcgaa
     5281 gctgttgcgc gcgccgcgcg cggcgacgag acccgcgatg atgagcccgg tgagccacac
     5341 ccacaggatc gcgaggtggc tgaacgccgc cgccgcgaag ggcttcattg gcccttcgaa
     5401 gtcgtgcttg gcgagcagca acaggactcc gccggcgaga ccgagcgcgg ggagcaacgc
     5461 gagcgcctgc gcgatcggcg tcggaatcca aatcacggcg cggttcgggc ccacgcgatc
     5521 ttgaattgca gcgccctgac ggcggtcggc ccaggtgagc aacaccgcga cgttcatcgc
     5581 gaacatcacg acgacgaacg cgcgaagtaa gaagattccg agttccggta gcgggatgtt
     5641 catccgttgg ctcctgccgt cggggccggg ggcgccgaag agcgcgccgt tttggtcact
     5701 gcattgagca ggtcttcaga gcgccgccaa cctggcgcgc gcccgagggc cttggcgagc
     5761 tcactcacga ggcgccaagc gggcttcgag tcaccttgcg atccgattgc cttttcactc
     5821 acttgcgcga gtcccttcgc gttcacgaag gttccgtccg cttcggccca cgtcgacgcc
     5881 ggcaagagca ccgccgcggc tttcgcccag ggtccctcgt gcatgctgag cgcgatgagg
     5941 gagcggatct tcgagaccgc gtcggtcttg tgcggcactt cggagccgag cgagacgacc
     6001 acgtcgagct tgccgcccga cacggcgctc tcgaactccg agaacgaacg gagcttgatc
     6061 gtatcgacga gctccgcgac gccggccgag tttgcgttct tgtcggggtt gatgaggatg
     6121 tcgtcgctgt agccctccgg gcgcccggtg acgaacacct gcgacaagcc cagcacttcg
     6181 cgcgcgagcc agatgagggc gaggttgtcc tcttgcgagt gctcggcgct cagcacgacc
     6241 gcgacgctcg cagcgtcctt gccgcgcaag agttccgccg ccttttcgac cgcggcttcg
     6301 cgggtgcttg cctcaccgcg cagccgcgcc gagagcacgc ggttttcgtg gatgcggcgg
     6361 tagtcgagca tgccgtcgtc gcacatccag tacttgttga cggcgaggtt ctcgcgcggg
     6421 cggtaacgat agaccttttg ctcgcgcggc tcgaaatcgg tgaatgaatt gcagccggtg
     6481 gcgcaaccag tgcagaccgt gcgcgccgag cgaaggaacc agacgcgcgc cttgaagcgg
     6541 aagtccttcg acgtgagcgc accgaccggg cacacgtact cggtcatcaa cgtgtacttg
     6601 ttgtcgagct ggcgcccggg cgagacggtg atctcgttca ggttgccgcg ctcgcgcacg
     6661 ctgagcaccg gatcgttggc gagctcggcg gtcacgcgca cgcagcgcgt gcacacgatg
     6721 cagcgctcgg cgtcgtacac gatggtagga ccgaacacga ctgccttcgg cttgtgcacc
     6781 ggctcgtcgc gcatgcgctt cttctggccc tggtgctcga gccagtagtc ctggagccgg
     6841 cactcgcccg cctggtcgca gatcgggcaa tctaccgggt gattcagcag caaaagctcc
     6901 tgcacggccg agcgcgcctt ggtgacgtgc tcgctcgtct ggctcttgac caccaagcca
     6961 tcagcggccg cgaattggca tgcgggctgg agcttcggct tcttctgcgg gacgtaatcg
     7021 ccctttttgg cgtcccactc gaggacgtcc agcatcatcg cggggcgccc gggcggaggc
     7081 atcacttcga cgaggcacat gcggcagttg gccgcgacgc tcaggccggg gtgccagcaa
     7141 taatgcggga tgtcgatccc gacgcggtgg gccgcgcgaa tgatggtgtc gccctgctcg
     7201 aacggaattt ctcggtcgtc cagcttgaaa gtaggcatca acgctccaag gtcgaattaa
     7261 gaaaatccgc tcgcatcagc ggtcgcactc gccaccgatc atgttcagcg agccgaagca
     7321 cgggaccacg tcggccatca tctggccggt gatcacgcgc tcgaggccgc cggtcacgta
     7381 gtagcaaggg ggacggatgc ggacgcggta cggggtgccg ctgccgtcgc tcacgagata
     7441 gaagccgtat tcgccgttgc cgccttcgat gtatccgtag gcctcgccgg cgggcagctt
     7501 gatgccctcc atcacgatct tgaagtgctg gatggtgcct tcgatggtcg tgtagacctc
     7561 ggacttcggc ggcagcacga tgcgcgggtc gtcgatgttg atggggccgc tgtcgggcat
     7621 gcgctgcaag cactgctcga tgatgagggc gctctgcttg atctcttcga tgcgcaccat
     7681 gaagcggtcg aagcaatcac tggcggagcc aaccggcacc tcgaactcga cctcgtcgta
     7741 cttgaggtac gggtgggctt tgcgcacgtc gtaaggaatg cccgtcgcgc gcaagcacgg
     7801 gcccgtccag ccgagcgaga tcgcgtcttg ctggctgatc acgccgacgc cctcgaggcg
     7861 atccaggaag atacggttgc cgaggaggag cttctccacc tcttcgagga tgtgcagaat
     7921 ctgacggacg acggcgcgag cgttctcttt gaagccgtcg gtgggaggag cggccatgcc
     7981 gccgacgcgc ccaaagctgt gcgtcatgcg cgcgccggtc tcttcctcca tcaagtccca
     8041 gatcatgtca cggcccttga tcatccagag gaagggcgtg aacgcgccga gctccatcgc
     8101 catggcgccg tcgcaggtca aatggtcgct gatgcgggcg agctcgccga gcgccatccg
     8161 ataccactga cagcgatccg gcactttgac gtcgaggagc ttctcgcaag cgagggcgaa
     8221 gcccacgttg caaagcatcg gcgagacgta gttcgtgcga tcgacgtacg ggaacacctg
     8281 cgtccaggtg ccgcgctcgc acatcttttc gaagccgcgg tgcaagtaac cgacctgcac
     8341 gtcgacgcgc tcgatgacct cgcccgagag ctccatcacg cagcgcacgg tgccgtgcat
     8401 cgcgggatgc gccggaccga cgttgagtag catcggctcc gccggcagct cgagctcgga
     8461 ctcttcgagc tcttgatcca aaggttccat cagttgctct ccacttcgtc aggagcgacg
     8521 cgaccgcgcg catgcgtttg gcgaccgaag gacatgccct cgtcggggcc gaacggcggc
     8581 agcttctcga tgttcggaac gtcgcggtac tcgacgagag gctgaatctt ctccgcggga
     8641 tagtccttgc ggagcgggta gccctcgaac tccgggtaca tcaggatgcg acgcaggtcg
     8701 gggtgaccca cgaacacgac cccgaacatg tcgtacgcct cgcgcgcggc ccagttcgcg
     8761 ctgccccaga gatccgcgag cgtatcgacg cgcgccgaat cgccctcggg atcgccgacg
     8821 cgcgtcttga tgcgcacgcg atgccctttg gagagcgaaa ggaggtgcgc caccacctcg
     8881 aagcgcggat cgcgatcggg gtagtcgacc acggtgaggt cgacgagcat ctgcatgttg
     8941 gcgcgcgggt cgtctcgcag aaattgcgcg acggcgtgcc acgacgccgc ctcgagcacg
     9001 atcgtgtcgt cgccgtgcgc ggcgtgcgtt tcgatgacgg cgttggggaa cttctgtcgg
     9061 accagatcga tcaggacttt cgccatatga aaagacctta cgaggcgcgc ttcacgttgg
     9121 ggggcgcgac ggccgcgagc gccgcagctg cacgaggccc atgttcggat cctgcaccgg
     9181 atcgtggcga ggcttgacga tcgcgggacg gcggtcgccg ctctggatct tgtcttggag
     9241 cagcatcaag ccgtcgagca cggcttcggg acgcggcgga cagcccggca cgtagacgtc
     9301 gcacgggatg attttgtcga tgcccgcgac cgtcgtgtaa ttgtcgtaaa agcctccgca
     9361 cgacgcgcag gtgccgaacg cgagcaccca cttcggctcc gccatctgct cgtagatacg
     9421 ccgcaaaatc ggcgcctgac gctgcgtgat ggtcccgacg acccacagga ggtccgactg
     9481 gcgcggcgaa aagcgcggcg cttccgagcc gaagcgcgaa aagtcgtagc gcgggctcga
     9541 taccgccatg aactccatcc cgcagcaggc ggtgacgaag gggtacatga acagcgagta
     9601 cttctgcgcc cacgcgagaa tcgactcgaa acgcgtggtg gcgaagcctt gttcgccgcc
     9661 cggctcgatg atgtgcgtag tcgtctgttt cagctctccc attcgagggc tcccttcttc
     9721 cagcagtatg cgagggccac gagcagagcg acgatgaagc cgaacatgct caaaaatccg
     9781 gtccaaccga ggctgcggaa caacgctgcc caggggtaca tgaacaccgt ctcgacgtcg
     9841 aaaacgacga acaagattgc ggtcagatag aacttcacgc tcattcggac gccgcgcgcg
     9901 ccttcggtgt cgttgccgca ctcgaagggc gtcatcttct cgggcgtcgg attcttgggc
     9961 ccgatgaatt gaccgccgag gaacatgacc gaggcgatca cgagcacgat gaggaacatc
    10021 agaaacatgg ggaggtagag ttccaccatg ggggtcgatc ctttggccga gccgcgcaaa
    10081 aggccgaaag aaaagcggcc tcggcgcggc tatctagtgc cggcgtaagt gtgctgtcaa
    10141 tgcgagcagc gccgtctggc gagaacccgc ggcgttgagg ctcaactcgc cgttttctcg
    10201 atgctttgac gcaacgcctg gaacggttcg ccgagaccct gcgacacgtg ctgtagcacg
    10261 ctttcgagag catcggccga caccccggcc gattgcccct cgagaacggc agcgtacgcg
    10321 gcgagcaagc gcccgcgctc catcaacgcc ctggcgagaa gcggccaaac gcgatcgcct
    10381 tcggccccga ggttcgccgc gcgacgcaac ggaccg
//
LOCUS       DASTKS010000946         4547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1493148, whole genome shotgun sequence.
ACCESSION   DASTKS010000946 DASTKS010000000
VERSION     DASTKS010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493148"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..362
                     /locus_tag="VFQ35_19920"
     CDS             165..362
                     /locus_tag="VFQ35_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932986.1"
                     /translation="MDVHFAEVRAELERVQRNRRVLADIKAGQKELARQKRLARGVGV
                     ALGEALARPAPARRENIGRYR"
     gene            375..875
                     /locus_tag="VFQ35_19925"
     CDS             375..875
                     /locus_tag="VFQ35_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932987.1"
                     /translation="MPDFTLVPKLNGTVFHTVPWTDPPAPHAAVTAAGGQSAWDALNA
                     SQQFALNMQHPDRPSRVTAIQGRQRFAWQATVNRPFQVWCQPDGGVAGCPDSALGGRL
                     FDAWFAESPELPLGPNYSVGYTSRLYYTPHLAGHYVLVVRRNDGRGGGVVLHIDVVDP
                     TASAPP"
     gene            1276..1437
                     /locus_tag="VFQ35_19930"
     CDS             1276..1437
                     /locus_tag="VFQ35_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932988.1"
                     /translation="MHETAAQAARVGVRLARLYGKPMTAGERMALAHLLDRCVALVQA
                     ALFVRKEVG"
     gene            complement(1508..2311)
                     /locus_tag="VFQ35_19935"
     CDS             complement(1508..2311)
                     /locus_tag="VFQ35_19935"
                     /inference="COORDINATES: protein motif:HMM:NF042503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6694 family lipoprotein"
                     /protein_id="HET9932989.1"
                     /translation="MNARQLSIVGALLMLGCGQPRLDASSDDAAKRSVAKMTEALPQA
                     DREKFGSALMLVAFNKMADGKGLLGLAAAASSATSASVLKPLEGKTATEIMAEADRIR
                     AEQAAKEHAQLLSEIAELEQKKKSADTAREALTKFVVSRSRFYKKQMAFLPQGQPVVE
                     LAVTNGTGKAVSRAYFVGTIATPGRSVPWLRANFNYEISGGLEAGESAQWALEPNMFS
                     EWGKVEAPPDAVFTVSVERLDGADGNPLYDASWSEENQERLDALKSKAQ"
     gene            2618..2827
                     /locus_tag="VFQ35_19940"
     CDS             2618..2827
                     /locus_tag="VFQ35_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932990.1"
                     /translation="MTDGTIPETGALAPLTDPWDRFAELNARGRELDDAFMRSLEGLE
                     KLLLGEAASSSTAARHHHGGGFPDE"
     gene            2820..3050
                     /locus_tag="VFQ35_19945"
     CDS             2820..3050
                     /locus_tag="VFQ35_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932991.1"
                     /translation="MNDANREEAALVAHEEARLVAREEARLEARAEMRAERVGVRAEG
                     GADDDGAFKRLYTDAFERHYVTELARLGQADP"
     gene            complement(3091..3309)
                     /locus_tag="VFQ35_19950"
     CDS             complement(3091..3309)
                     /locus_tag="VFQ35_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932992.1"
                     /translation="MSQSSAYFATVSGALPASDGSVVPVACSAIRRAIFRRAAGLLSV
                     SKLNHRRTLSPSTVTVSWHHHDLPRNSR"
     gene            complement(4410..4481)
                     /locus_tag="VFQ35_19955"
     tRNA            complement(4410..4481)
                     /locus_tag="VFQ35_19955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4447..4449),aa:Gly,seq:gcc)
BASE COUNT          808 a         1533 c         1477 g          729 t
ORIGIN      
        1 ccttttgcgc cgccgcgaca tgctccgcga tcaaatgctc gatacgccta cccaactcgg
       61 atgtcgtgtt cgtcatgcac gaccaaatcc cacgccccca cgccgtcgct caatcccacc
      121 cctgaacggt tacgctcaaa gcggcggacc tcgcaacgcc cgacatggac gtgcactttg
      181 ccgaggtgcg cgccgagctc gaacgggtgc agcgaaaccg ccgcgtgctc gctgacataa
      241 aggccggcca gaaggagctc gcacggcaaa aacgactcgc gcgtggcgtc ggagtcgctc
      301 tcggcgaggc gctcgcacgc ccggcgcctg cgcgacgcga aaacatcggc cgctataggt
      361 aaggactcac aaccatgccc gacttcacgc tcgtgcccaa gctcaacgga acggtttttc
      421 acaccgtacc gtggacggac ccgcccgcgc cgcacgccgc agtcacagca gcgggcggac
      481 agtccgcgtg ggacgcgctc aacgcgagtc agcagttcgc gctcaacatg cagcaccctg
      541 atcgaccgtc gcgcgtgacc gcgatccaag gtcggcagag gttcgcgtgg caagcgacgg
      601 tgaaccgacc gtttcaagta tggtgccagc ccgacggcgg cgtcgccgga tgccctgact
      661 cggcacttgg cggtcggctg ttcgacgcgt ggttcgccga atcgcccgag ctgccgctcg
      721 gcccgaacta cagcgtcggc tacacctcgc ggctctacta cacgccacac ctcgccggtc
      781 actacgtgct cgtcgtgcga cgcaacgacg gccgcggggg tggtgtcgtg cttcacattg
      841 acgtggtcga tccgaccgcg tcggcgccgc catgacgacg gcgcggttca cgcagccggg
      901 ggcacgctga tgtcaggcga gcaccatgtc ctcgggacga gaagcccttg ggtcgatgtc
      961 acggttctgt gcggagttcc gccgcgtgcc gttgagggaa tgccagccgg acctgacgag
     1021 agtgacagca ccgacgacga gctcgcgccc atccagcgaa cgtcgacgga acaccgaatc
     1081 cgagagtgcg cgcacggctt cgcaattccg cggccgggtg cacccgcggt cgagttcgtg
     1141 tgccccgagc ccggttgtcc ggcgaatagc acttcagtcc gcgcgacgct cacggcgtgc
     1201 gcgcgccgtc tgcactgcat cgaggtctgc agcagaccac ggaagcgaag cgggaaacgg
     1261 ctgaaggttg actcggtgca cgaaaccgca gcgcaagcgg ctcgcgtcgg agtgcggctt
     1321 gcgcggttgt acggcaaacc gatgaccgct ggtgaacgaa tggcgctcgc gcacttgctc
     1381 gaccgatgcg tcgcgctcgt tcaagcggct ctgttcgtac ggaaagaagt gggctgatgc
     1441 cggctacaga cgcgaagcca gcgtcaagct cgctttcgac ggcgtgacac tgttcctgcc
     1501 tgtcgtttca ctgggccttg ctcttgagcg cgtcgagtcg ttcttggttc tcttctgacc
     1561 acgaagcgtc gtagagcggg ttcccgtcgg cgccgtcgag ccgctccact gagacagtga
     1621 agacggcgtc cggcggtgcc tcaacctttc cccactcgct gaacatgttc ggttcgagcg
     1681 cccactgcgc gctctccccc gcctccaagc cgccggatat ctcgtagttg aagtttgctc
     1741 gaagccaagg cacggaacga cctggtgtcg cgatcgtgcc gacgaagtag gcacgagaga
     1801 ccgcctttcc ggttccgttg gtcacggcga gttcgaccac gggttgaccc tgcggcagga
     1861 aggccatctg cttcttgtag aagcgcgacc gcgacacgac gaacttggtg agcgcctcgc
     1921 gcgcggtatc ggccgacttc ttcttttgtt ccagctccgc aatctcactg aggagctgag
     1981 cgtgctcctt tgctgcttgc tccgcgcgga tgcggtcggc ctccgccatg atctccgtcg
     2041 cggtcttccc ctcgagcggc ttgagcactg aggcgctggt tgcgctgctc gccgctgctg
     2101 cgagaccgag gagtcccttg ccgtcggcca tcttattgaa cgcgacgagc atcagcgcgc
     2161 tcccgaactt ctccctatcg gcttgcggca acgcctcggt catcttggca accgaccgct
     2221 ttgcggcatc gtcagacgag gcgtccaaac gcggctgccc gcatccgagc atcaacagcg
     2281 cccctacgat tgatagctga cgtgcgttca tgaggtcccc tttcgacgcg gtcgcgccac
     2341 aagacaatca ccccatcttc ctgcgatggg cgctctatgt ctagccgccc ggtgtgcggc
     2401 gttttgggcg aagattgccc cctcgcggaa agctcgcaca agggcaccgt tcacgcgggc
     2461 cttgcgcgta gctaccgcgc cggcttcgtt tgcggtgttg aactaggacg ggcttgacga
     2521 cgccagctcg aacgcgaatc ctgaacgctg aacccaataa gtggggcccg cggtgcttgc
     2581 aacacccgga cccctggcca ccgaaaggaa cctttcgatg acggacggca cgataccgga
     2641 aacgggtgcg ctcgcgcccc taacagaccc ctgggaccgt ttcgcggagc tcaacgccag
     2701 agggcgcgag ctcgacgacg ctttcatgcg ttcactcgag gggctggaaa agctgctcct
     2761 cggggaggcc gcgtcgagct cgacggccgc gcggcatcat cacggtggag gtttccccga
     2821 tgaatgatgc gaatcgcgaa gaagcagcac tggtggccca cgaagaagca cgactcgtcg
     2881 ctcgcgaaga agcgcgcctc gaagcgcgtg cggagatgcg cgccgaacgg gtaggtgtgc
     2941 gagccgaagg cggcgctgac gacgacggcg cgttcaagcg gctgtacacc gacgcgttcg
     3001 agcgtcacta cgtgacggag ctcgctcgac tcgggcaggc agatccgtga acgaagcgca
     3061 aaagcgaaag ggcggtcgcc cgcgcacggg ctacctcgag ttccgcggca agtcgtggtg
     3121 gtgccagctc acggtcaccg tcgacggcga gagcgtgcgc cgatggttca acttggagac
     3181 ggacagcaag cccgctgcgc gccggaagat ggcccgcctg atcgccgagc acgcgactgg
     3241 cacgacgctc ccgtccgacg ccggcaaggc tcccgagacg gtcgcgaagt acgccgagga
     3301 ctggctcaag gcgcgcgaga aacgagagct cccgagcgcg gcgaacgagc gccgctactt
     3361 cgagcgcgtg tggaagaagg agatcggcgc gctcgaactc ggcgccgttc gcgcgggcgt
     3421 gctccaagcc gttctcgact gcgccgtcga cggcaccatc acgaagaaga acggcgagcg
     3481 ctactcgcgc gagagcatca agcacatgcg cgcggtgacg ctgcgcttgc tcgagtcggc
     3541 gtggcgcgag gagctcgtca ccgagaacgt ggccaagcgc accgtcgtgc ccgagatgga
     3601 agaggaccga cgcccgcgcg ctgtgctcac cgacgacgag cttggccggc tcatcgcgca
     3661 ccccgacgtc gacgccgaga tcaaactgct cgtgctcacg tcgcgtcaca tcggcgggct
     3721 tcgcaccggc gacctgaacc ggatggattg gaacgcgttc tcaccgaact tcgagacgtg
     3781 tacgttcgtc cgtcgcaaga ctcggaagaa gcggcccgag cccgagacgc acgccgtgcc
     3841 ggaggcggtg cgcccgttcc tcgacgcgtg gtggcgcgct gcaggctgcc cggcgaccgg
     3901 ccccgtgttc ccggcacgcc gcggcgaccg ggcggggaag gagaagaagc aggcgaagca
     3961 gagctacgcc gaacgcctcc gccgcgagct tcgaatcgcc ttcggcatcg acgcgcggga
     4021 ccccttgacc ggcgcctgga cggcggacgc ccgcgcgccc acgcctcggg agcgggaact
     4081 gctcgagggg tcggccaccg tccagccggt ggacttccac agcacccgcc gcgcctacgc
     4141 gacgaccctg gctcacgtcg gggtgaacga gcagacggcg atgcagctga ccgggcactc
     4201 ggactcgaag acgcaccggc gctaccaggc ggcggaccgc gtgctcgcgc tgccgcccga
     4261 agccgtcgtc gtggtgccgc ccgacgcgct cgccgttttc gctgcaaaca ggaatcgcac
     4321 cagcgatggt gcgaaaccga ggggcttcga aagaaaaaac cggctgactc atcagagcca
     4381 accggctgtt ttctctccga ctttttcgga gcgggagaag ggatttgaac cctcgacgtc
     4441 caccttggca aggtggcact ctaccgctga gttactcccg cggaagagcc ggcggtgtac
     4501 ctcccgctct ctttcgtgtc aagcacttgc cggaggtcgg ccggacg
//
LOCUS       DASTKS010000947         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_374554, whole genome shotgun sequence.
ACCESSION   DASTKS010000947 DASTKS010000000
VERSION     DASTKS010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374554"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFQ35_19960"
     CDS             <1..222
                     /locus_tag="VFQ35_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932993.1"
                     /translation="ADAEGVLREALDIAGPSGPDRARVLSALAHVAYGRHRNSEALGY
                     IEQAIDVARQSGAHELVAAFSDTRRSWAS"
     gene            241..1272
                     /locus_tag="VFQ35_19965"
     CDS             241..1272
                     /locus_tag="VFQ35_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932994.1"
                     /translation="MASVAQSFITRWDLDKTYLRSRFDTLSDLVSSALEKVEQKRTVP
                     GATEVLRELGRSGGRVHILSGSPRQFRGRLSQKLRIDGVSWDELTLKPNLSNLLRMRL
                     YALRDQLGYKLPELLEARARDEERFGSGLRMREVLVGDDSESDAFVYSLYADLSLGEV
                     PQRELENVLAAGNIGERTIARCKKALARLRHDGAIECILIHLDGQTPPSLFSRYGARL
                     VPFYNYLQAAFVLLDRGFLSEHSTLEIASAFQRSYGFTADTAARSYLDLVRRGHLGEG
                     ARVRLTHAVSELAAPSREERALVESLGKLSIDGAPLSVPRRQRPDYLELAAFHGGGRN
                     RRRPGQRFG"
     gene            1276..1842
                     /locus_tag="VFQ35_19970"
     CDS             1276..1842
                     /locus_tag="VFQ35_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932995.1"
                     /translation="MKLEAKLRASVITLLLVLGFIAGWPKVSPRLLERLSPSLAESLA
                     RVPELQARLLAPIAPVAHAFGIYSENWALFATTGGTRNRIHVEGRPRGSSEFVLLYRV
                     HDPEHRYLAELLEYRRVRNLWNPHRSGLSTGYEPFARWLVERTLREHPEFDAVRIRMQ
                     EGKILPRGAGFEPTGRFQFEVQAERPAP"
     gene            1839..2822
                     /locus_tag="VFQ35_19975"
     CDS             1839..2822
                     /locus_tag="VFQ35_19975"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9932996.1"
                     /translation="MIARWVELWSRREPATSLACVRIGIAAVLLVDLLAAWWLGALPA
                     LWTPPPSGFGWGALSEHPPVLVQWFGAREGVAIAAWLLAVLSAIGVAFGFCFRVTSWL
                     LVFAMLELEGLMPDGDGIDGLVRIVLPLLALSDAHAVLSFDARWKRGRGKPLDTEILA
                     WPRYLIVVQLLWVYFSAAHHRGKASWGFSGEFAALGDVLGDPHFSRFAPGAFAPLYPL
                     LQLAGFLTMVFEFSAPLFLLFSYYQRRPDRGGFIGAWVRRLRLRDVWLALGVGLHLGI
                     AATMELGIFPFGILALYPALLPPDELEALHARARQLIDQYKASTRPTTNLR"
     gene            complement(2780..>3193)
                     /locus_tag="VFQ35_19980"
     CDS             complement(2780..>3193)
                     /locus_tag="VFQ35_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9932997.1"
                     /translation="FELNQLSGATHEAVTSDVFEYLEGARQRGREFDLIICDPPSFAA
                     AKEKQYAALRAYAKLNALGFSVVAPGGLYAAASCTAQVSPEAFRATLAQAAERAGVRF
                     QIIHDVGQPVDHPVFAGHPEGRYLKFVVGRVLALY"
BASE COUNT          472 a         1076 c         1047 g          598 t
ORIGIN      
        1 gccgacgccg aaggcgtcct gcgagaagcg ctcgacatcg cgggaccgag cggccccgat
       61 cgcgcgcgcg tgctctccgc gctcgcgcac gtggcctatg gacgccaccg caacagcgaa
      121 gccctgggct acatcgagca agccatcgac gtggcgaggc agtcgggcgc tcacgagctc
      181 gtcgcggcgt tctccgacac gcgccgcagt tgggccagct gacccagtag taggtgcctc
      241 gtggcgagcg tcgcacagag cttcatcacg cgctgggacc tcgacaagac ctacctgcgc
      301 agtcgcttcg atacgctctc tgacctcgtg agctcggcgc tggaaaaggt cgagcagaag
      361 cgcaccgtgc ccggagcgac ggaggtgctc cgcgagctcg gccgcagcgg tgggcgcgtg
      421 cacatcctga gcggaagccc gcgccaattc cggggacgcc tgagccaaaa gcttcgcatc
      481 gacggggtca gttgggacga gctcacgttg aaacccaacc tctcgaactt gctgcgcatg
      541 cgcctttacg ctttgcgtga tcagctcggc tacaagttgc cggagctact cgaagcgcgc
      601 gcccgcgacg aggagcgctt cggctccggg ctccgaatgc gcgaagttct cgtcggcgac
      661 gactccgagt ccgacgcttt cgtgtactcg ctctacgccg acctctccct cggcgaggtg
      721 cctcaacgcg aactcgaaaa tgtcctcgcc gcaggaaaca tcggcgagcg aacgatcgcc
      781 cgctgcaaga aggcgctcgc caggttgcgc cacgacggcg cgatcgagtg catcctgatt
      841 cacctcgatg gacagacccc accgagcctg ttctcgcgct atggcgcgcg gctcgtccca
      901 ttttacaact acctgcaggc cgcgttcgtg ctgctcgatc gcggcttttt gagcgagcac
      961 agcacactcg aaatcgcgag cgcgtttcag cgttcgtacg ggtttacggc cgacacggcg
     1021 gcgcgcagct acctcgactt ggtgcgccga ggccacctgg gcgaaggcgc gcgcgtgcgc
     1081 ctcacgcacg ccgtctccga actcgcggcg ccttcgcgcg aagagcgcgc gctcgtggag
     1141 tcgctcggca agctgtcgat cgatggcgct cccctgagcg tgccgcgtcg gcaacgtccc
     1201 gactacctcg agcttgcagc gtttcacggc ggaggtcgca atcgccgccg ccccggccag
     1261 cgcttcggct gatcgatgaa actcgaggcc aagctccgtg cgagcgtcat cacgttactc
     1321 ttggtgctcg gcttcatcgc gggctggccc aaggtctcac cacgcctgct cgagaggctc
     1381 tcgccgtcgc tcgcggagtc gctcgcgcgc gttccggagc tccaggcgcg cctgctcgcc
     1441 ccgatcgcgc cggtcgcgca cgcgttcggc atctatagcg aaaactgggc gttgttcgcg
     1501 accacgggcg gaacgcggaa ccgcatccac gtcgagggcc gcccgcgcgg tagcagcgag
     1561 ttcgttctgc tctaccgtgt gcacgacccc gaacaccggt acctcgccga gctactggag
     1621 tatcgccggg tgcggaacct ctggaacccg catcgcagcg gtctctcgac tggttatgag
     1681 ccgttcgctc gctggctcgt cgagcgtacg ttgcgagagc atcccgagtt cgatgcggtt
     1741 cgcatccgca tgcaagaggg aaagatcttg ccgcgcggcg ctggcttcga gccgacgggg
     1801 cgctttcagt tcgaggtgca agcggagagg cccgcgccgt gatcgcgcgc tgggtggagc
     1861 tctggagtcg ccgcgaaccc gcaacgtcgc tcgcctgtgt acgcatcggg atcgcggcgg
     1921 tgctgttggt cgatttgctc gcggcgtggt ggttgggggc cctccccgca ttgtggacgc
     1981 cgccaccgtc gggttttggt tggggagccc tcagcgagca tccgccggtg ctagtgcagt
     2041 ggttcggcgc gcgcgagggc gtcgccatcg cggcgtggct gctcgcggtc ttgtctgcca
     2101 tcggcgtagc cttcggcttt tgttttcgcg tcacgagctg gctgctggtc ttcgcgatgc
     2161 tcgagctcga agggctgatg ccggatggcg acggcatcga cgggctcgtt cgcatcgtgc
     2221 tccccctgct cgcgctatcc gacgcgcacg ccgtgctctc gttcgatgct cggtggaagc
     2281 gaggccgcgg caagccacta gatacggaaa ttctggcttg gccgcgctac ctcatcgtgg
     2341 ttcagttgct gtgggtgtac ttctccgccg cgcaccaccg tggcaaggcg tcttggggtt
     2401 tctccggtga gttcgccgcg ctcggcgacg tgctcggtga tccccatttt tcgagattcg
     2461 cgccgggcgc cttcgcgccg ctctacccgt tgctgcagct cgccggtttt ctcacgatgg
     2521 tgttcgagtt ctcggcgccg ctgttccttc tgttcagcta ctaccaacgt cgcccggacc
     2581 gcggcgggtt catcggcgcc tgggtgcgtc gcctgcggct tcgcgacgtg tggctcgcct
     2641 tgggggtagg gctccacttg ggcatcgccg ccacgatgga gcttggcatt ttccctttcg
     2701 ggatcctcgc gctgtatccg gcgctgctac ccccagacga gctcgaggcg ctccatgcgc
     2761 gcgcgcgcca gctcatcgat cagtacaagg cgagcacgcg accgacgacg aacttgaggt
     2821 agcgcccctc ggggtgaccg gcgaaaaccg ggtgatcgac gggttggccc acgtcgtgaa
     2881 tgatctgaaa gcgtacgccc gcgcgctcgg ccgcctgcgc cagcgtggct cggaaagcct
     2941 caggagatac ttgcgcggtg caactcgcgg ccgcgtagag ccccccggga gctaccaccg
     3001 aaaagccgag cgcgttgagc ttcgcgtaag ctcgcagggc cgcatattgc ttctcttttg
     3061 cggcggcgaa gctcggcggg tcacagatga tcaagtcgaa ctctcggcct cgttgccgcg
     3121 ctccctcgag gtattcgaac acgtcgctcg tcaccgcctc gtgggtcgcg cccgaaagct
     3181 gattgagctc gaa
//
LOCUS       DASTKS010000948         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1045088, whole genome shotgun sequence.
ACCESSION   DASTKS010000948 DASTKS010000000
VERSION     DASTKS010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045088"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3306
                     /locus_tag="VFQ35_19985"
     CDS             <1..>3306
                     /locus_tag="VFQ35_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9932998.1"
                     /translation="NPVKIFQAYRKVRRGKTLEWKASSVTAGQDMRGWPVIDFMRAYG
                     GPAKVGLVMLLFLGWLVLLGAPLDLFGLNSVGVFIASFLTAWFYAWYSALPYSTDSGV
                     PQYEMASREFWGLTLLGTAGVGISVALFALGLYPMPALEPSTGYLALFLSGTCAFAAL
                     FPSYYRIKRHFMLSSAPQGGGPGRRFSGILVALTVGAAIASLFVASPGLRDRAHRFFA
                     KDPARFRVPAVERAVYASVGVALKRTRTVEPEPLLSADGTQVTLSQTPQGPIFDAKEL
                     VGIPSPRPKRVALRDQRPLPELFALPPAARIPINKPRRDFVLATAKLRDKPQPPHAEH
                     LEAVLEARRRARDMAPRKLSAAELAEEAELLEKADYPWISRDDLAKLGSPDPKGARLS
                     LVEMARVYHFDEIKALWDRVPAASDGNPVPRARFALLVDGIDGAASLGLEPTAEQVQA
                     FLAAEREVSARWSREFPNVPLGAVRERVAGTPSGAFQVARHELLYGLTFEDVARRLRL
                     DYVNAAWNRAPLAPTLRRVFRDRVEVEQPDRLELDWGENEEARIRFHGLETEQLVASK
                     ILAPTLGTKLGSLEELEATLRLVERALDAANGGHTDALSAALARTFRIEGPSDDGANS
                     DSTTALLRARLWAQTMSGRLGQIAARVAANTPLPGDASDQALLSEVSTLRYPSEDAGE
                     AERRGLDWLVLVDANETYRELVDGYDRFRREFGERGLSVKGLSQPPQLTPEEQWRDLF
                     AVWRELPAKYPHIPVRADLVAEFICQTAYFSSADGKRARTPREFIQDFASVFADVDRL
                     MAETPPAMVQELTDAASLKTQGRLSRSADARRFFAWWNVSALTRVVRAAKGAERVYDT
                     LDSHGIARSWAELTSSALTRWPHLPWRAPGFSEYFVTLEHVAHLPLPALWQRFEPQLN
                     SAEALSVRHVVPAPAFEALIEHRVSEKTGMPNFDPVSRRGNALLALSELAGTAAKNGV
                     RFDDAHPNKLGVSLVHLHDGLSRSYPSLYWDADGIIESYLMLSLVNGWDERRTLREFD
                     AEWALANALAKTGLLARLGEIASKDVGKLSPTDRWVRTFIDTQALRLEKKAGMAPRGT
                     RARAMNALLALASL"
BASE COUNT          541 a         1121 c         1066 g          578 t
ORIGIN      
        1 caacccggta aagatcttcc aagcgtaccg aaaggtgcgt cgtggcaaga ctctcgagtg
       61 gaaagcgtcg tcggtcacgg ccgggcaaga catgcgcggc tggcccgtca tcgatttcat
      121 gcgagcgtac gggggcccgg cgaaggtcgg cttggtcatg ctgttgtttc tgggctggct
      181 cgtgctgctc ggcgcgccgc tcgatctctt cgggttgaac agcgttggcg tgttcatcgc
      241 gtcgtttttg accgcgtggt tctatgcctg gtactcggcg ctcccctact ccaccgacag
      301 cggggtccct cagtacgaaa tggcgagccg cgaattttgg gggctcacgc tgctcgggac
      361 ggcaggcgtc ggcatctcgg tcgcgctgtt cgcgctgggt ctctacccca tgcccgccct
      421 cgagccgtcg accggctacc ttgcgctctt cctgagcggg acgtgcgcat ttgcagctct
      481 cttcccgagc tattaccgca tcaagcggca cttcatgctt tcgagtgcgc cgcagggtgg
      541 cggacccggg cggcgcttct cgggcatcct cgtcgcgttg accgtcggcg ccgcgatcgc
      601 gtcgttgttc gttgcttcac cgggcctacg cgatcgcgcg catcgcttct ttgcgaagga
      661 ccctgcgcgc tttcgcgtgc cggcggtcga gcgcgccgtc tacgcttcgg tgggcgtagc
      721 gctcaagcga acgcgcaccg tcgaaccaga gccgctgctc agcgcggacg gaacgcaggt
      781 caccctgagc cagactcccc agggacccat cttcgacgcc aaagagctcg tggggatccc
      841 ttctccgcga cccaagcgag tcgcgcttcg cgaccaacga cctctccccg agctctttgc
      901 tttgcccccg gccgcgcgaa tcccaatcaa taaaccgcga cgcgacttcg tactcgcgac
      961 cgcgaagctt cgcgacaaac cgcagccgcc ccacgccgag catctggagg cggtgctcga
     1021 ggcgaggcgc cgcgcgcgtg acatggcgcc aagaaagctt tctgccgccg agcttgccga
     1081 agaggccgag ctgctcgaaa aagccgacta tccgtggatt agccgcgacg acctcgccaa
     1141 gttgggctcg ccggacccga aaggtgcgcg cctttcgctc gtcgagatgg cgcgcgtcta
     1201 ccacttcgac gaaatcaagg cgctatggga ccgcgttccc gccgcgagcg acggcaatcc
     1261 ggtgccgcgg gcgcggttcg cccttctcgt cgatgggatc gacggtgcgg cgagcctcgg
     1321 gctcgaaccc actgccgaac aggttcaggc tttccttgcc gccgaacgcg aggtcagcgc
     1381 gcgctggtct cgcgagtttc cgaacgtccc gctcggcgcg gtgcgcgaac gcgtcgcggg
     1441 cactccgagc ggtgcctttc aggtagcgcg ccacgagctc ctctatgggt tgacgttcga
     1501 agacgtcgcg cgccggttgc gcctcgacta cgtcaacgcc gcctggaacc gcgcgccact
     1561 cgcgccgacc ttgcgacgag tctttcgcga tcgcgtcgag gtcgagcaac cagatcgact
     1621 ggagctcgat tggggcgaga acgaagaggc tcgcattcgg tttcacggac tcgaaacgga
     1681 gcagctcgtt gcgagcaaga tcctcgcgcc gacgctcggc acgaagctgg gtagcctcga
     1741 agagctcgaa gcgacgctcc gcctcgtcga gcgagctctg gacgcggcga atggcgggca
     1801 caccgacgcg ctctcggcgg cgttggcgcg caccttccgc atcgaagggc cgagcgacga
     1861 cggagccaac tccgactcga cgaccgcgct cttgcgggcg cggctctggg cccagaccat
     1921 gtccgggagg ctagggcaaa tcgcggcgcg ggtcgctgcg aacacgcctc tccctggaga
     1981 cgcgagcgat caggcgctct tgtcggaagt gtctacgctc cgctacccga gcgaggatgc
     2041 tggcgaggcc gaacgccgcg gcctcgattg gctcgtattg gtcgatgcga acgagaccta
     2101 tcgcgagctc gtcgacggct acgatcgctt tcgccgcgag ttcggggagc gcggcctcag
     2161 cgtgaagggg ctgagccaac cgccgcagtt gacccccgaa gagcagtggc gcgacctgtt
     2221 cgcagtctgg cgcgagctgc cggcgaagta tccccacatc ccggttcgtg ccgacctggt
     2281 ggcggagttc atctgccaaa ccgcgtactt ctcgagcgcg gacggcaagc gcgctcgaac
     2341 ccctcgcgag ttcatccaag atttcgcgag tgtgttcgcg gacgtcgacc ggctgatggc
     2401 cgaaacaccg cccgcgatgg tccaggaact gacagacgcg gcgtcgttga agacgcaagg
     2461 ccggctcagc cgcagcgccg atgcgcgacg cttcttcgca tggtggaacg tgtcggcgtt
     2521 gacccgcgta gtccgcgccg cgaagggagc agagcgcgtc tacgatacgc tcgactctca
     2581 cggcatcgcg agaagctggg ccgagctcac gagctcggcg ctcacgcgct ggccccacct
     2641 gccgtggaga gcgccgggct tttcggagta cttcgtcact ctggagcacg tcgcgcacct
     2701 gccgctaccc gcgctgtggc agcgcttcga gcctcaattg aatagcgcgg aggcgctcag
     2761 cgtccgccac gtggttcccg cgcccgcctt cgaagcattg atcgagcatc gcgtctccga
     2821 gaagactggc atgccgaact tcgatccggt gagccgccgc ggcaacgccc tcctcgcctt
     2881 atccgagctc gcaggcaccg ctgccaagaa cggagtgcgc ttcgacgacg cccatccgaa
     2941 caagctcggc gtgtcgctag tccatctgca cgacgggctt tctcgaagct acccgagcct
     3001 ctactgggac gcggacggta tcatcgaatc ttacttgatg ctctcgctcg tgaacggctg
     3061 ggacgagcga cgcacgctgc gcgaattcga cgccgagtgg gctctcgcca acgccctcgc
     3121 aaaaacgggg cttctcgctc gcttgggcga aattgcctcg aaagacgtag gcaagctctc
     3181 gccaacggac cgttgggttc gcacgttcat cgacacccaa gcgctccgcc tcgaaaagaa
     3241 ggcggggatg gctcctcgcg ggacgcgcgc gcgtgcgatg aacgccttgc tcgccctggc
     3301 aagcct
//
LOCUS       DASTKS010000949         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_374706, whole genome shotgun sequence.
ACCESSION   DASTKS010000949 DASTKS010000000
VERSION     DASTKS010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374706"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFQ35_19990"
     CDS             <1..621
                     /locus_tag="VFQ35_19990"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9932999.1"
                     /translation="LELSPGVQAKLLRAIEQKEVFSLGENRARSVDVRFISASQLPLA
                     RAVQSGTFRSDLRARLEALVIELPTLAARRSDIVPLFQQLLTRHGYSKAPRIDGKLAE
                     RLSLYDWPMNVRELENVARRIASAVPGESLLTNELAREWLGLDEANSVTPPASRKGVA
                     AYSDEELEKLQEALARCSGNVARAAAELGITRPRVYRMLRAAGLKE"
     gene            636..2102
                     /locus_tag="VFQ35_19995"
     CDS             636..2102
                     /locus_tag="VFQ35_19995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933000.1"
                     /translation="MEPADLAEIEDFVGVGSVLAGKYRIERLLGVGGMGAVVAAHHEQ
                     LHERVAIKFLLRRTGQNERLAARFLREARTASKLKSAHVARVFDVDSRSDGTPFIVME
                     YLEGETLGSRFRRDRELSRSSIVDDILEACVALAEAHAQGIVHRDLKPSNLFVAEEAS
                     GHWGVRVLDFGISKVVEPPSSDDVITQDLPIGSPPYMSPEQFTQPSSVDHRTDIWSLG
                     VVLYEGVTGRLPFVGTSFAEISASVLQRSPEPMDDRPELEPVLRGCLAKERERRFPDI
                     ASLAEALSPHAGERGGRALVVLQSWQRRRVQRQESVADVERSSAPPAPNKAMHEETST
                     ATGTADATHVPASRPRAERRRLVAGALAVLSVLLGGLTYRALLGARAPTRSATVTAVS
                     IPTSPGAIPDGARPVPSSAPPPPTSTLAPDAALSAVGAAASVVAPPPLAPSARDEPAR
                     RQRGVQTSSPTGSPPRNLKSVPPAAAGRAFDPVFDERR"
     gene            2164..>2369
                     /locus_tag="VFQ35_20000"
     CDS             2164..>2369
                     /locus_tag="VFQ35_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933001.1"
                     /translation="MRASAFVIACCLLTAPLTFACGSSGEDIDLLDPAQPQYGKTYAE
                     WVADWIAYIYRMSPPECFNAIVDDT"
BASE COUNT          399 a          763 c          789 g          418 t
ORIGIN      
        1 ctcgagctat cccccggcgt ccaggccaag ctgctccgtg cgatcgagca aaaggaggtg
       61 ttttcgttgg gcgagaatcg ggcgcgctcg gtagacgtca ggttcatctc cgcgagccag
      121 cttccgcttg ctcgcgccgt gcaaagcggc acgttccgca gcgacttgcg ggctcgcttg
      181 gaggcgctgg tcatcgaact gccgacgctc gcggcgcgtc ggtccgatat cgtgccgctg
      241 tttcagcagc ttctgacccg acacggatat tccaaagcgc cgcgcatcga tggcaagctc
      301 gcggagcggc tctcgctcta cgactggccg atgaacgtgc gtgagctcga gaacgtcgcg
      361 cgtcggatcg cgagcgccgt cccgggcgaa agcctgctca ccaacgagct cgcacgcgaa
      421 tggttggggc tggacgaagc gaacagcgtc acgccgcctg ctagtcgcaa aggcgtggct
      481 gcttactccg atgaggagct cgaaaagctc caggaggccc tcgctcgctg ctctgggaac
      541 gtggcccgcg ccgccgcgga gctcgggatc acccgcccac gcgtctaccg catgctgcgc
      601 gccgcgggac tcaaagagta accggggaat cgaacgtgga acctgccgac cttgctgaga
      661 tcgaagactt cgttggcgtg ggcagcgtcc tcgccgggaa gtaccgcatc gaacggctgc
      721 tgggtgtcgg tgggatgggc gcggtggtcg cggctcacca cgagcagctc cacgaacgcg
      781 tcgcaatcaa gttcttgctg cgccgcaccg gacaaaacga gcgcctcgcg gcgcgcttct
      841 tgagagaagc gaggacggcc agcaagctga agtcggcgca cgtcgcgcgc gtgttcgacg
      901 tggactcccg ttcggacggc actccgttca tcgtgatgga atacctcgag ggtgagacgc
      961 tcgggagccg ctttcgccgc gatcgcgagc tcagtcgcag ttcgatcgtg gacgatattc
     1021 tcgaagcgtg cgtcgcgcta gcagaagcgc acgcgcaggg catcgttcat cgcgatctga
     1081 agccttccaa tttgttcgtg gcggaggagg cgagcggaca ttggggcgtg cgtgtgttgg
     1141 atttcggaat ttcgaaggtc gtcgagccgc cgtcgagcga cgatgtcatc acgcaagatt
     1201 taccgatcgg ctcgcccccg tacatgtctc ccgagcagtt cacgcagcct tcgagcgtgg
     1261 accaccgcac cgacatctgg tcgctcggtg tcgtcctata cgagggagtc acgggcaggt
     1321 tgccgttcgt tggaaccagc ttcgcggaga tctccgcgag cgtgctgcaa cgctcacccg
     1381 agccgatgga cgatcggccc gagctcgagc cagttttacg agggtgcctg gccaaggaaa
     1441 gggagcggcg ttttccggac attgcttcgc tcgcagaagc gctgtcgccg cacgccggcg
     1501 aacgcggggg gagggcgttg gtagtgctcc aaagctggca gcgacgtcgg gttcagcgac
     1561 aggagtccgt cgccgacgtg gagcgctcga gcgctccacc tgccccgaac aaggcgatgc
     1621 acgaggagac cagtacggcg accggcactg ccgacgcgac gcacgtaccc gcgagccgtc
     1681 cacgtgcgga acggcgccgg ctcgtggcgg gagctctcgc cgtgctcagt gtcttgttag
     1741 gtgggctcac ctatcgagcg ctgctcggag ctcgagcacc gactcgcagc gcgaccgtga
     1801 cggccgtatc tatcccgacc tcgcccgggg cgattccgga tggcgctcgc ccagttcctt
     1861 cgagtgctcc gcctccgccg acgagcacgc ttgctccgga cgcagcgctt tcagcggtcg
     1921 gcgctgcagc gtcagtggtc gcgccgccgc cgctcgcgcc atccgcccgc gatgaacccg
     1981 cacgccgcca gcgaggggta caaactagct cgcccactgg ctccccgccg cgaaacctaa
     2041 agtctgttcc gcccgcggcg gccgggcgcg cgttcgaccc ggtgttcgac gagcggcgct
     2101 gagcgcggag ggacctgtat gcgtcgactt gatcttggca gcgagctgtg cagatctcgg
     2161 cgcatgcgag cctcggcatt cgtcattgcg tgttgtctgc tgaccgcacc gcttaccttt
     2221 gcgtgcggat cgagcggaga agacatcgat ttattggatc cggctcagcc gcagtacggc
     2281 aagacgtacg ccgagtgggt cgccgattgg atcgcctaca tctacaggat gtctccaccg
     2341 gagtgtttca acgccatcgt ggacgacac
//
LOCUS       DASTKS010000950         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_374829, whole genome shotgun sequence.
ACCESSION   DASTKS010000950 DASTKS010000000
VERSION     DASTKS010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374829"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFQ35_20005"
     CDS             complement(<1..715)
                     /locus_tag="VFQ35_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933002.1"
                     /translation="MWRYALIFAAALSGCAAALYVAVHRYEWLGPLVANSLRSVFGTG
                     FVAKLEDLVYGVEDRVNRWTKRDQAPKAYWSVPTNTAHAPPTTPSAGNPASGAPRAPE
                     LVETGPPPFHPVDPGPVLKRWSAQGDGVWVPIVDPRRPDEAPYMYKTLLHPDATRSWA
                     EVFVVAIDVPRVDLFVVAGSQEPKTDDPKTASYVRVAKVPEAQHEELLAAFNGGFMTE
                     HGGYGMKVDGVVLVQPKPKA"
     gene            1417..>2222
                     /locus_tag="VFQ35_20010"
     CDS             1417..>2222
                     /locus_tag="VFQ35_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933003.1"
                     /translation="MTLNAVRVGQTFSPEIARRALDELADQGRYGDMRVTVEPRGNGV
                     ALRLHVVARRVIVGIRISGVEGDPDDLLRAANVRTGNELLATELPDLALRVKRELIRH
                     GYPYALVNIGTLDTDDPLGVVLTIGVERSAPERISARNFAVWPDPKAQGMGKLLGTFG
                     VRVGDRADLDRVEAEAEALEERLRAAGFHEARVQHTIERGARGTVLNLQIYAGLRTRI
                     QFEGHRHFDVAELEAALDVANNQDRAPSALAERIRVFYVARGFLDAAVDVR"
BASE COUNT          372 a          715 c          766 g          369 t
ORIGIN      
        1 aagccttcgg cttcggttgt acgaggacga caccgtcgac cttcatgccg tagccgccgt
       61 gctcggtcat gaagccgcca ttgaaagcgg caagcaattc ctcgtgctga gcctcgggaa
      121 ctttggcgac ccgcacataa gaggcggtct tgggatcgtc ggtcttgggt tcttggctgc
      181 ctgcgaccac gaacaaatcg acgcgcggca cgtcgatggc caccacgaac acctccgccc
      241 aggagcgcgt ggcgtccggg tgaagcaacg tcttgtacat gtacggcgcc tcgtcaggac
      301 ggcgcggatc gacgatcgga acccaaacgc cgtctccctg ggcggaccag cgcttcaaga
      361 cgggccccgg atcgacgggg tgaaaaggcg gaggcccagt ttcgacgagc tccggggcgc
      421 gcggcgcgcc cgaggcaggg ttgcccgcgg agggcgtcgt cgggggggcg tgagccgtgt
      481 tcgtgggcac ggaccagtac gctttcggcg cctgatctcg cttcgtccag cggttcacac
      541 ggtcttcgac gccatagacc aagtcttcga gcttcgcgac gaagccggtt ccgaatacgc
      601 tgcgcaagct gttggcgacc agcggtccga gccactcgta gcggtggacg gcgacataca
      661 gtgccgctgc gcagccgctc agcgcggccg caaagattag cgcgtaccgc cacacggcgc
      721 gccgccggga gcgtctcgga ggcgcggaac gccgcgagct cgagaggctt gcttgaatca
      781 tgcggaagag ttgcgggccg ggtctcgcgg ggttttcgca ccgcgcgcgg cttttcgcaa
      841 gctttcggcc tgccccagcg gcgtcgcggt gctctccggt ggcggttcga cgttttggcc
      901 ccggcggcgt ggtccctcac cgcgagcaaa tcccgactat aggtgaaacg tcggtctttc
      961 tcgactatga cgcggcgaat gggcctcggt ccgcaccact tccgctcgtg cctcggcgtg
     1021 gcgctcgcga tcgagctagg ggcggcttcg ctcgccgctc agcctagagt cgcggtgccg
     1081 gagacgggga agccggtgcc gcccattgcc gcgccacccg cggaagcgcc gccctcagac
     1141 gtcccgcccc gggaagcatc cgatggaacg gaatcacccg ctccggaggt cgaggcaact
     1201 cgcggtcccg acaccggcgt cgacgcggac gttggtactg cagaccgtag ccctgcagcc
     1261 gccgggcggg acgaagccgg gcgagacgag gctaggcgag acggagcgag tcgagagccg
     1321 agtctcgcgc cgctggatgc aacggttcgc caatatatcg gtcggaacat cgtagcgatc
     1381 gaagtcgtct atgaggccgc actctggcgc ggcccggtga cgctcaatgc ggtgcgagtc
     1441 ggtcagactt tctcgcctga aatcgcgcgc cgggcgctcg acgaactcgc cgatcaaggc
     1501 cgttatggcg acatgcgcgt cacggtcgag ccgcgcggca acggtgttgc gctgcggttg
     1561 catgtagtgg cgcgccgggt gatcgtcggc attcgaatct cgggtgtcga aggtgatcca
     1621 gatgacctgc tgcgcgccgc aaacgttcgg accggcaacg aactgctcgc gaccgagctg
     1681 ccggacctgg cgttgcgcgt aaaacgcgag ctgatccgcc acggatatcc gtacgcgctg
     1741 gtgaacatcg gaacgctcga cacggacgac ccgctcggtg tcgtcttgac gatcggcgtc
     1801 gaacgcagcg caccggagcg catatccgcg cggaatttcg cggtctggcc agatcccaag
     1861 gcccagggta tgggtaagtt gctgggtacc ttcggcgtgc gcgttggcga tcgcgcggat
     1921 ctcgatcgag tcgaagccga ggccgaagcg ctggaggagc gcctgcgcgc ggcgggcttc
     1981 cacgaagcgc gcgtgcaaca cacgatcgag cgtggggcac ggggtaccgt gctcaatctg
     2041 caaatttacg ccgggcttcg tacccgcatc caattcgagg gccatcgcca cttcgacgtg
     2101 gccgagctcg aagccgcgct cgacgtcgcc aacaatcaag accgagctcc ctccgcgctg
     2161 gcggagcgga tacgagtgtt ttacgtcgcg cgcggctttc tggatgcagc cgtcgacgtg
     2221 cg
//
LOCUS       DASTKS010000951         8224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_598711, whole genome shotgun sequence.
ACCESSION   DASTKS010000951 DASTKS010000000
VERSION     DASTKS010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598711"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1251)
                     /locus_tag="VFQ35_20015"
     CDS             complement(109..1251)
                     /locus_tag="VFQ35_20015"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9933004.1"
                     /translation="MQVSVIAIFIAGVLTFASPCILPLIPVYLGVLGGAVASKRSARP
                     VVAAAAFAAGLSTVFVALGAAASAFGGVLMQHRTALLFLSGTLMVVFGLRALGVLRVG
                     VLDRDARPGLERLRVVGGLGSAFLFGAAFALGWSPCIGPVLASVLGFVASRGTSPLQG
                     AAYLGVYAAGISAPLIVVAAFAPTAIQLLRRFRSAIPRLEIATGALMIAVGAWTLWSA
                     RDGLLSSAQVAPVAAASHRAPSNEPTSCDGNAANGQSHLCALPNGGPVAAEVHEAAGA
                     RMLEFVSSHCPVCVRMAPVISQAERACDGFGARIQRVDVATPEGRALALRHRVVGTPT
                     FVFVNDEGAESERIVGEETPENLRAAIESAFALRCAVLSPRPSNSG"
     gene            complement(1442..2761)
                     /locus_tag="VFQ35_20020"
     CDS             complement(1442..2761)
                     /locus_tag="VFQ35_20020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9933005.1"
                     /translation="MPSRARTTAADPTLLLAVIGELGGAAIVLDGELAVRGATPAASR
                     MLGVDIPTGVALPKLLCGRAEERPVADALLRGKAVSAKVPWLSPAGERLIGVRTTPVD
                     GGWLVLLHDDSLVSEEEADAPVLFEGMWTRDPGMKALYRLIERAAASEANVLIRGETG
                     AGKELVAKALHERSARKSGPFRAINCAALPPNLLESELFGHERGAFTGAVRDSLGHFR
                     AAQGGTLFLDEVAELPLELQSKLLRVVETRTVIPVGARDAIPVDVRLVAATHRSLRKE
                     VEAGRFRADLMFRLRVVPLFLPPLRARAGDVALLTHKLLEGLAVRSPRRVEHVADSAL
                     SLLESYPWPGNIRELRNALEYALVMGDGPVLVPSDLPPEIADPRLEEAVPLVNRPAPI
                     APEGDPDPERARIQRALDRAGGNRERAAKLLGVSRATLWRKLRKANA"
     gene            complement(2772..3239)
                     /locus_tag="VFQ35_20025"
     CDS             complement(2772..3239)
                     /locus_tag="VFQ35_20025"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HET9933006.1"
                     /translation="MSRDRSWIWGIVFGFVLSRIGATDPRAIHGMFTLTDPQLLVVIA
                     LAIAITAVVFFGFRRGRFTTRSGAPAALSPKPWQRGLVVGGLLFGVGWAVSGACPGTA
                     LTQIGEGRLSGALTFLGILVGAELVRRVTRRKASSPAPVSAPPAGEAKLAAST"
     gene            complement(3248..4447)
                     /locus_tag="VFQ35_20030"
     CDS             complement(3248..4447)
                     /locus_tag="VFQ35_20030"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HET9933007.1"
                     /translation="MLVGVALGLLGGGGSLLSVPVLVVALGLDVRTAIATSLVVVGFT
                     SIVALVPRIFAKQVDFKAGLTLGAAGMIGAYVGGKLARHVPSAVLMLLFAGIMLATAA
                     AMFRERPQPRDVKPSAHPIVAGILRGVAMGLLTGLVGAGGGFLVVPTLVLFARLEMPT
                     AVGTSLLVIAMQSFAGFAGSAGHVSVPLALAGSVAIAAAAGAVVGQRLSRRFEARTLR
                     RLFASFVLVAGTFTLAHQLPDALWSSPWFQAAFVQRWPGWLGGIALATVVVGLLYFEN
                     RLLGVSTGCAELCESVRSAAARRSWRLRFLAGILAGGVLAAVFARRVPSFGLSLTGVF
                     ASASFPVQALALFAGGVLVGAGARLAGGCTSGHAIVGVAQGARASFVATACFMAAGFV
                     TTWLFSG"
     gene            complement(4375..4809)
                     /locus_tag="VFQ35_20035"
     CDS             complement(4375..4809)
                     /locus_tag="VFQ35_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9933008.1"
                     /translation="MIQAAQILVRHATPLEVQSMLTRSRIIDVREPHEFFGADGHVPG
                     AELVPLASLEVTAAQWTRDEPLVVVCRSGTRSFRAAQLLNGLGFKHVTNLVGGMIAWR
                     ALDLPIETRLPRSSRRFSSCSLASRSACWAEAARCSACLSSS"
     gene            complement(4813..5520)
                     /locus_tag="VFQ35_20040"
     CDS             complement(4813..5520)
                     /locus_tag="VFQ35_20040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9933009.1"
                     /translation="MLFRQLFDPVSSTFTYLLADEGTRAALLIDPVREQVERDLELIR
                     DLGLRLEMVLETHIHADHVSAAGVLRSRTGARVVASELGAPCVDVRVRHGSRLSLGSI
                     AIEVLATPGHTDDSISFLIGDRVFTGDALLIRGSGRSDFQNGDPEVLWDSLVNVLFQL
                     PRETLVFPGHDYKGRTVSTIGEEVAFNPRVAGRTREQFVEIMRNLGLPPPAQLDVAVP
                     MNRACGDGNPRVTAERS"
     gene            complement(5752..6324)
                     /locus_tag="VFQ35_20045"
     CDS             complement(5752..6324)
                     /locus_tag="VFQ35_20045"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9933010.1"
                     /translation="MPNLSRWSSRLASAEVHALVSLLPLFGTACSATAMPSTTATTPS
                     SAVAPTTTASSAAAGASAEATPSPIATRSPAPAAATSATGISRTLLDQADLPDMPGWE
                     TRLFLIEYAPGVAAPKHHHPVGGVGYVLSGSFESAFEGQKPTIVREGQSFRDLPEASH
                     VLFRNADNSKSLKFIIMYVVRKGEPVLMTP"
     gene            6581..7429
                     /locus_tag="VFQ35_20050"
     CDS             6581..7429
                     /locus_tag="VFQ35_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933011.1"
                     /translation="MVDLPAGSNVSATARTSTPYTPGFRTSAYTVLGALIAVGCASSH
                     PSGPVFGGQGAVVDPGQRAAQVEAQAPAGVCDGVPASTDAHFEDFEDGDNRLFGAFER
                     EGYWYTATDHTPGGSVFPPDGKFEPATLPENEATPENRLAAHFTAEGYKDWGVVWGVT
                     LRHVGAAKCPLNAGNFAGLKFRVRGKGDVQVRFGMPETIATEYGGSCTEKCYDMHGTT
                     LHLTDTWQVREVHWDRLQQGGWGKSVRFDPKRLLSLEFRAAPDKMPVEVWLDDFDWIP
                     KEAGKG"
     gene            7426..>8224
                     /locus_tag="VFQ35_20055"
     CDS             7426..>8224
                     /locus_tag="VFQ35_20055"
                     /inference="COORDINATES: protein motif:HMM:NF019264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1595 domain-containing protein"
                     /protein_id="HET9933012.1"
                     /translation="MTAFDRAIPLLLLACLDATGCAAATAPPSAPVAALLPARLRRLS
                     NVEYERTANSLTRLNESFRDELPPDERQDGYTVNERQTVTSYYAAALERLAERLAGLA
                     VTRNLDALLPCPASMPPSSRCVGEALDALGETAFRRPLTAEERNGVRSLYERGASLPA
                     TGDAPRDPRARGVALVLSTVLRSPSLLYLSELGDARLVESADEHAGDKATVATLNSYE
                     VASALSYTVTGAPPDAELFAAAAVPNLLLRPDVREAEARRLLSRSSTR"
BASE COUNT         1576 a         2786 c         2675 g         1187 t
ORIGIN      
        1 gtgcgacggc aagcggcgcg cgaaggccac ctcagtaact actcgcgcag cgagcgcggc
       61 aagctccttc gttgagagcg gcgccgtcgt tcgcccgtcg cgagcagctc agcccgagtt
      121 cgaagggcga ggcgacaaca cggcgcagcg cagcgcaaac gcgctctcga tggccgcgcg
      181 gaggttctcg ggagtctctt cgccgacgat gcgttcgctc tcggcgcctt cgtcattgac
      241 gaacacgaac gtgggcgtgc cgacgacgcg gtgccgaagt gcgagtgcgc gcccctccgg
      301 agtcgcgacg tcgacgcgct gaatgcgggc gccaaagccg tcgcaggcgc gctcggcctg
      361 cgaaatcacc ggtgccatgc ggacgcacac cgggcaatgc gatgaaacga actcgagcat
      421 gcgcgcgccg gctgcttcgt gcacctcggc cgcgacaggg ccgccgttcg gaagcgcgca
      481 cagatgactt tgaccgttgg cagcgttgcc gtcgcacgac gtgggctcgt tactcggggc
      541 tcgatgcgaa gccgccgcga cgggagcaac ttgcgccgac gagagcaggc cgtcgcgcgc
      601 tgaccaaagc gtccacgcac cgacggctat catcaacgcg ccggtggcga tttcgagacg
      661 cggaatcgcg ctgcggaagc gtcgcaagag ttggatggcg gttggcgcga aggcggccac
      721 gactatcagg ggcgccgaaa tgcccgccgc gtacacgccg aggtaagcgg ctccttgtaa
      781 cggcgacgtc cccctcgatg cgacgaaacc gagcaccgat gccagcaccg gcccgataca
      841 cggagaccag ccgagcgcga acgcggcgcc gaagaggaac gcgctcccaa gccctccgac
      901 cacacgcaag cgctcgagcc ccggccgcgc atcgcgatcg agaacgccca cacgcaagac
      961 tccgagcgct cgcagaccaa agaccaccat caacgtcccg ctcagaaaca acagcgccgt
     1021 gcgatgctgc atgagcacgc ccccgaacgc gctcgcagcc gcgccgagcg ccacgaatac
     1081 cgtcgagagc cctgccgcaa aagccgctgc cgccacgacg ggccgagccg agcgcttgga
     1141 agcgaccgcg ccgcccagga ctcccaggta cacggggatg agaggcaaaa tgcacggcga
     1201 cgcaaaggta aggacgccgg cgatgaagat cgcgattacg gaaacctgca ctcgaacctc
     1261 tctgagacgg aacttcgtaa cgcctacgac gcatacccgc gcgcggttac tcgaaggcgc
     1321 aatttcttcg tggagttgta gtgtaccgcc aaagttcttc ggcgcccgga ccgacaccaa
     1381 cgcccgaaca ccgaccacgt ccccagtcgt aacccttggg accggaaact cggcgagtgc
     1441 gtcacgcgtt cgccttccgg agcttacgcc agagcgtcgc tcggctcact ccgagcagct
     1501 tggcggcgcg ctcgcggttg ccccctgctc gatcgagggc acgctgaatg cgcgctcgtt
     1561 cgggatccgg atcgccctcg ggggcgatgg gcgcgggccg attgacgagc ggcaccgcct
     1621 cctcgagcct cggatcggcg atttcaggtg gtaggtcgga ggggaccagc accggtccgt
     1681 cacccatcac gagcgcgtat tcgagcgcgt tgcgcaactc tcggatgttg ccgggccagg
     1741 gatagctctc gagcaagctc aacgccgagt cagccacgtg ctccacacgg cgaggcgaac
     1801 gcaccgcgag cccttcgagg agcttgtgcg tgaggagcgc cacgtccccc gcgcgcgctc
     1861 tgagcggagg cagaaacagc ggcactacgc gcagccgaaa catcagatcc gcacggaagc
     1921 gacccgcctc gacctccttc cgcaagctgc gatgcgtcgc cgccaccaac cgaacgtcga
     1981 ctggaatggc gtcgcgagct cccaccggaa tgacggtgcg cgtttcgacg acgcgcaaaa
     2041 gcttcgattg gagctcgagc gggagctctg ccacctcgtc cagaaacagc gttccgccct
     2101 gcgcggcgcg gaaatgaccc aagctgtcgc ggaccgctcc ggtaaacgcg cctcgctcgt
     2161 gcccaaagag ctcgctctcg agaaggttcg ggggcaacgc ggcgcaattg atcgcgcgga
     2221 acggccccga tttacgcgcc gagcgctcgt ggagcgcctt tgccacgagc tctttaccgg
     2281 cgcccgtttc acctcgaatc aagacgttcg cttcgctcgc agcagcccgc tcgatgaggc
     2341 gatacagcgc cttcattcct gggtcgcggg tccacatgcc ttcgaaaagc accggcgcgt
     2401 ctgcctcctc ttcggagacg agcgagtcat cgtgcagcag caccaaccag cctccatcga
     2461 ctggcgtggt gcgcacgccg atcaggcgct caccagcagg agacagccag ggcaccttcg
     2521 ccgacactgc tttcccgcgc agcagcgcgt cggctacggg gcgctcttcc gcccgtccgc
     2581 agagcagctt gggtagcgcg acgccggtcg ggatatcgac gcccagcatc cgcgaagccg
     2641 ccggagttgc ccctctcacc gcgagttccc catcgaggac aatcgcggca cctccgagct
     2701 cgccaattac cgcgagcagg agcgtcggat cggcggcggt agtgcgggct cgagatggca
     2761 tcggtgacag tctatgtcga tgcggcgagc tttgcctcgc cggcgggtgg ggccgagacg
     2821 ggggcgggac tcgaagcttt tcgtcgcgtc acgcggcgaa cgagctccgc accgaccagg
     2881 atcccgagaa atgtcagcgc tcccgaaagg cgaccctcgc cgatctgcgt gagcgcggtg
     2941 ccgggacagg ccccgctaac cgcccagcca acgccgaaca gcagcccgcc cacgacgagc
     3001 ccgcgctgcc acggcttggg gctcagcgca gccggcgcgc ccgagcgagt cgtgaagcgc
     3061 ccgcgacgaa agccgaagaa gaccacggcc gtgatggcga tggcgagcgc gatcacgacc
     3121 aaaagctgcg gatccgtgag cgtgaacata ccgtgaatcg cccgcgggtc ggtggcgccg
     3181 atacgcgaaa gcacgaaccc gaaaacgatt ccccaaatcc aagatctgtc gcgagacatc
     3241 actcacctca ccctgagaac agccacgtcg ttacgaaacc ggccgccatg aagcacgcgg
     3301 tcgcgacgaa gctcgcacgc gcgccctgag caacaccgac gatggcgtgg ccggaggtac
     3361 agccgccagc gagccgcgca cccgcgccga ccagaacacc tcccgcgaac agcgccagcg
     3421 cttgtacggg gaacgacgcc gacgcgaaca caccggtcaa ggagagtccg aaactcggaa
     3481 ctcggcgcgc gaagaccgcc gccagcacac ctccagcgag aatgccggcg agaaagcgca
     3541 gccgccacga cctgcgcgcg gcagcgctgc gtaccgactc acagagctcc gcgcatccgg
     3601 tggagactcc gagcaagcgg ttttcgaagt agagcaagcc gacgaccact gtcgcgagcg
     3661 cgatgccgcc gagccagccc ggccagcgct gaacgaacgc ggcctggaac cacgggctcg
     3721 accagagcgc atccggcaac tggtgcgcca acgtgaacgt ccccgcaacg agcacgaagc
     3781 tcgcaaagag ccggcgcaac gtacgcgcct cgaaccgccg cgaaagtcgc tgacccacca
     3841 ccgcgcccgc cgccgccgct atcgccaccg aaccggcgag cgcaagtggc acactgacgt
     3901 gcccggcgga ccctgcgaac ccggcgaacg attgcatcgc gatgaccaac aacgaagtgc
     3961 cgacggccgt aggcatctcg agccgcgcga acaacaccag cgtcggaacg acgaggaagc
     4021 cgccgcccgc gccgacgagc cccgtgagca atcccatggc cacgccgcgc aagatgccgg
     4081 ccacaatcgg gtgcgcgctc ggtttcacgt cgcggggctg ggggcgctca cgaaacatgg
     4141 cggctgccgt cgcgagcatg atccctgcga acagcaacat gagcacggct gacgggacgt
     4201 ggcgagccag tttcccgccg acgtaggcac cgatcatgcc cgccgcgccg agcgtcaacc
     4261 ctgccttgaa gtcgacctgt ttggcgaaga tgcgcggaac cagtgcgacg atgctggtga
     4321 atccgaccac gacgagcgag gtcgcaatgg cggttcgaac atcgagtccg agagctacga
     4381 cgaggacagg cacgctgagc agcgagccgc ctccgcccag caagccgagc gcgacgccaa
     4441 cgagcacgaa gagaaccgcc gcgaggatct tggaagcctc gtctcgatgg gcaggtcgag
     4501 cgccctccag gcgatcatac ctcccaccaa gttcgtgacg tgcttgaacc cgagcccgtt
     4561 caagagctgc gcggcgcgaa aggagcgggt ccccgagcgg cacacgacga cgagcggttc
     4621 gtcgcgagtc cattgcgcag cggttacttc caggctcgcg agggggacga gctccgcgcc
     4681 ggggacgtgc ccgtccgcgc cgaagaactc gtgtggctct ctgacgtcga tgatccgcga
     4741 gcgcgtgagc atcgactgga cttcgagcgg cgtcgcgtga cgcaccaaga tttgcgcggc
     4801 ttgtatcatt gctcagctcc tctcggccgt gacccgtgga tttccgtcgc cgcaagcgcg
     4861 attcatggga accgcgacgt cgagttgcgc gggcggcggc aagcccaggt ttcgcatgat
     4921 ctctacgaac tgctctcggg tgcgaccagc cacgcgcgga ttgaaggcga cttcttcgcc
     4981 gatcgtcgag acggtgcggc ccttgtagtc gtgcccgggg aacaccagcg tctcacgcgg
     5041 caactgaaac agaacgttga cgagtgagtc ccacagcacc tcgggatcgc cgttttgaaa
     5101 gtcggagcga ccgctcccgc gaatcaggag cgcgtcaccg gtaaagacgc gatcgccaat
     5161 caggaagctg atgctgtcgt cggtgtggcc cggagtcgcg agcacctcga tggcgatgct
     5221 tccgagcgaa aggcgtgacc cgtgacggac ccgaacatcg acgcaagggg cgccgagctc
     5281 gctcgcgacg acacgcgcgc ccgttcgcga acgcaacaca cccgctgcgc tcacgtggtc
     5341 ggcgtggatg tgcgtctcga gcaccatctc gaggcgcaga cccaagtcgc gaatcaattc
     5401 caagtcgcgc tcgacctgct cacgcaccgg gtcgatcaac agcgcggcgc gtgtgccttc
     5461 gtccgcgagc aggtacgtga aagtcgaact gaccgggtcg aacagttgtc gaaacagcat
     5521 gggatgaacc tctgggccca cccatagcga gcgacgtgcc aacgcaaaaa cgccgacatt
     5581 ttcgcaacta ttgcaacaga ttgaaacatc tcgagcgcct ggtgcaacac cgtgaaacag
     5641 cgctcgctcc gacgggaatg aagcgttcgg accagcgcgc cggagccgca cccggctctc
     5701 cctgcgaccc ggctagatcg gattccggcg ccgcgtcgca gctccacgtc gtcagggcgt
     5761 catcaacacg ggctcgccct tgcgcacgac gtacatgatg atgaacttga gcgacttcga
     5821 gttgtcggcg ttacggaaaa gcacgtgaga ggcctcgggt aagtcgcgaa agctttggcc
     5881 ctcgcgcacg atggtcggct tctgaccctc gaacgccgat tcgaagctcc cgctgagcac
     5941 gtagccgacg cccccgacgg gatggtggtg cttcggcgcg gcgacgccgg gcgcgtactc
     6001 gatgagaaaa aggcgcgtct cccagcccgg catgtcgggc aagtcggctt ggtcgagcaa
     6061 cgttcgcgag attcccgttg cgctcgtagc ggcggcgggc gcagggcttc gcgtcgcaat
     6121 ggggcttggc gtcgcttcag cgctagcccc cgccgcggcg ctcgacgcgg tcgtggttgg
     6181 cgcaactgcg ctgctcggcg tggtcgcggt ggtgctcggc atggctgtgg cagagcaagc
     6241 cgtaccaaac agtggaagca gcgaaaccag ggcgtgcacc tcagccgatg caagacgaga
     6301 agaccaacga gataggttcg gcatggcgac ttctggcgtt ggcgatgtgg gccatccgaa
     6361 gccgccaacg actacagatg tagtctcacc ggccaaccac ttcaactaca attgtagttc
     6421 ctcctcggag tgagcgacct acacgcgtcc gaagcaaaac gcgggtccac cctgaaattc
     6481 tggaaacgcc gtcaaattcc gagcctatgg cctcgggcga gcggcaccgc aagtgacgga
     6541 tgtaccgatc ggaacgcgcc gcgtctgaca tcctttgagc atggtcgact tgcccgccgg
     6601 ttcgaacgtc tcagccaccg ctcggacgag cacgccgtac actcctgggt tccggaccag
     6661 cgcctacacc gtgctcggcg ccctcatcgc ggtgggatgt gcgtcgagtc acccgagtgg
     6721 gcctgtcttt ggcgggcaag gagccgtcgt cgaccccggg cagcgcgcgg cccaggtcga
     6781 ggctcaggca ccagcgggag tgtgcgacgg agtgcccgcg agcacggatg cgcacttcga
     6841 agacttcgag gatggagaca accgactttt cggtgcgttc gagcgtgaag gttactggta
     6901 cacggcgacg gatcatacgc cgggtggcag cgtgttcccg ccggatggaa agttcgagcc
     6961 ggcgaccctg ccggagaacg aagcgacgcc ggagaatcgc ctggcggcgc acttcactgc
     7021 tgagggttac aaagattggg gggtggtgtg gggtgtcacg ctgcggcacg tcggtgcggc
     7081 caagtgcccg ctgaacgcag gcaacttcgc ggggctgaag ttccgcgtcc gcggcaaggg
     7141 cgacgttcag gttcgctttg ggatgccgga aacgatcgcg accgaatacg gcgggagctg
     7201 taccgagaag tgctacgaca tgcacggaac gacgctccac ctgaccgata cctggcaagt
     7261 gcgagaagtg cactgggatc gcttgcagca gggcgggtgg gggaagtcgg tgcgcttcga
     7321 tccgaagcgc ttgctttcgc tcgaatttcg cgccgctcct gacaagatgc cggtcgaagt
     7381 ctggctcgac gatttcgact ggatccccaa agaagctggc aaaggatgac cgcgttcgat
     7441 cgcgcaatcc cgctgctgct cctcgcgtgc ctggacgcga cgggctgcgc cgcggcaacg
     7501 gcgccgccaa gcgcgccggt tgccgccctg cttcccgctc ggctccgccg gctgagcaat
     7561 gtcgagtacg agcgcactgc gaactccctc actcgcctga acgagagctt tcgcgacgag
     7621 ctccctcccg acgagcgcca agatggctac accgtcaatg aacgacagac ggtcacctcg
     7681 tattacgccg ccgcgctcga gcgcctcgcg gagcgcctcg ccggtctcgc cgtcacgcgc
     7741 aacctcgatg cattgctgcc ctgtcccgct tcgatgccgc cgagttcgcg ctgcgttggc
     7801 gaggcgctcg atgcgctcgg cgaaacggct ttccgcagac cactcaccgc ggaggagcgc
     7861 aacggcgtgc gttcactcta cgaacggggt gcgagtttgc cggcaacggg cgacgctccg
     7921 cgcgacccgc gcgcgcgcgg cgtagcgctc gtgctttcga cggtgctccg ctcgccgagc
     7981 ctcctctact tgagcgagct cggcgatgca cgtctggtgg agagcgcgga cgagcacgcc
     8041 ggcgataagg cgaccgtcgc gacgttgaac tcgtacgaag tcgcgtcggc gttgtcgtac
     8101 acggtcacgg gagcgccgcc cgacgccgag ctgttcgcgg ccgcagccgt gcccaacctc
     8161 ttgctgcgcc ccgatgtgcg tgaagcggag gcgcggcggc tgctttcacg gtcgagcacg
     8221 cgcc
//
LOCUS       DASTKS010000952         6638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1269490, whole genome shotgun sequence.
ACCESSION   DASTKS010000952 DASTKS010000000
VERSION     DASTKS010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269490"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFQ35_20060"
     CDS             complement(<1..414)
                     /locus_tag="VFQ35_20060"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET9933013.1"
                     /translation="MNDAEPNAGDVAKDDWGKGTRDPAERRFLQGPRPRGTEFVEAAR
                     IFAECVKGFRRLHFVGPCVTVFGSARFKESHPYYELAREVGRELARAGFTVMTGGGPG
                     IMEAANRGAKEAGGYSVGSNISLPIEQKPNPYLDRW"
     gene            complement(460..1404)
                     /locus_tag="VFQ35_20065"
     CDS             complement(460..1404)
                     /locus_tag="VFQ35_20065"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HET9933014.1"
                     /translation="MRARLSFVVALFSSSLASAQPAAPDDPSAKTVSPAEGQSSERPG
                     AARAVGGAGEIRSPNAPQPEDEPELDALTFHRANYFITGFTRDTQVKFQFSIKYDLWP
                     SKGHHTVYFAYTQRSLWDLFSRSSPFRESNYAPELFYAHYHSEVHGEPGPGCGLFSEQ
                     AGIAHESNGEASDSSRSWNRLFVDVAAACNSKHVYGLVELRVWYPLGVSDNESITQTQ
                     GYGELGLAVGFDDVAAHTNGLVSLALRKGANFDLAKGSVTLDVRWRPTYQRLFGESWK
                     LAPYVWFQLFTGYGETLGSYDRAQTSLRVGIGFTDRSR"
     gene            complement(1611..3041)
                     /locus_tag="VFQ35_20070"
     CDS             complement(1611..3041)
                     /locus_tag="VFQ35_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933015.1"
                     /translation="MLVGTASFATRAGAQRLDVAGTVVCQDPVRGATLASDVLVVYAD
                     DPSIGSRTTRGHGTFRLIFPRPRKIRARNITLEFWPNQQKEEHTWFVPPSPTNVLDGI
                     PAFVVPEDVMLDARCDQIDPKAATKAEAQENMQAAGFASHVLQLLALIPAAAGAAADE
                     EVLSISKERLPIIASEPGNETGPLDLARTTDFPFLGFRYAPRTYAGSAIATNPAAVAL
                     GDGLEIQTNGAVRGIQPYRFSVVADWADGRNFGIGFAYFVQVNSKERAIQYGQGESLV
                     ADTQSHAHTFVLAPAYQLSPGLAVSLAPTLTLQYLEDPKDIARTTTVYGRPEGGTTVP
                     TRTVVEDEVNTVWRNRGRVDAAASIVYDVTTNIRLGAGIQNILGTTERVRGRKLSERT
                     VGAGFSTAYYRLQVGADVEYSQAHGFDGTAGASFFVSNGFEFGAGYLTKGGLYRLWSR
                     VGPVVVGVARNQDDAWFVNLGSRVVY"
     gene            complement(3133..3753)
                     /locus_tag="VFQ35_20075"
     CDS             complement(3133..3753)
                     /locus_tag="VFQ35_20075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9933016.1"
                     /translation="MASARSLSQPSKPVWPLVLGAAFLGFTVLFLMGLILLSTAGHEV
                     PCSSRFLVSAVLALTVAVGSSFLGGTAAARGQLPWKGAEKHPIRFAVTGGVAVFVITL
                     TLGSQLYNGGADCLPSLPTVKPVKVTGRVLGEQGAPVAAARVSVTGVGARSTNSDGSF
                     EIPVSGSSGPFSLSVYAPGYSPLTTSFELEGHDVKLADLQLGAQKK"
     gene            4234..6015
                     /locus_tag="VFQ35_20080"
     CDS             4234..6015
                     /locus_tag="VFQ35_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933017.1"
                     /translation="MKTSKAGLSVPSCAHLAAGGAVLRGALVFLAASASVGCDSDETP
                     KAWNALASCIVGKDAASPIAARVQALRLVQLANASGDAAAKDGWPQRCNGYADDLFAT
                     LPTSGESAILRGKLRDKLGCTETKGSCKLRNDASLLTTTTDLFDAAKSSNLTTQAASG
                     VPAPTAATPPSLDAKSWKSLSPKPVKVSGPTLTATGRGVLVLKGTEGRSRPSGCEFTD
                     GFTKVRCIEANSSVPELPVQSIEVVSDPKGIFAAGLTDDGLVAYDLQTGEKSEVRGRS
                     GHLLRDGVVVEKSAKEDVSAEPPRGLPGRDAKGGKAAKAKPKAKPKGPVKEEGYEAIE
                     LTAGKASKPIKLPIKASVGDPIALGDQIVFLSPLESGAELGTFTVSHARLKPGPAIKG
                     SFSGALHGCQAGSALAVAAYGPRSGQNNAKPTAGDGKTQLSVSLFRGSGWSKALETTL
                     PFDRSFESDLVCTKDGATLVWVRNAEGSVQVGRVDCTAEGCKSSDVKLPGVESKWWWG
                     VGPLGDKTFLLWRSTLGETRLRVAPLAELANAKDVLAFDTPDFGGPTAGEVTSLLTAD
                     AALLTFRGEQPVALRIGNDGALRLISP"
     gene            complement(6085..>6638)
                     /locus_tag="VFQ35_20085"
     CDS             complement(6085..>6638)
                     /locus_tag="VFQ35_20085"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9933018.1"
                     /translation="FSVQSTHMLFGAETLGILSRREQLKAFRGTLLAVAFWTGLGFIV
                     GPAAFLFCFVVPLLVANAIVMSFIFTNHSISPLTDVNDPLANSLSVTLPRWLEWLTLG
                     FGFHVEHHLFPAMSARHARRVHDVLVARWPKQYRSLPLGRALRLLYRSPRVYKTHSLL
                     FDPRSGREWPISPTNGAANAPSS"
BASE COUNT         1269 a         2196 c         2062 g         1111 t
ORIGIN      
        1 ccaacgatcc aaatatgggt ttggcttctg ctcgatgggg agcgaaatgt tgctgccgac
       61 ggagtaacca cccgcctcct tcgcgccacg gttagccgcc tccatgattc cgggcccgcc
      121 accggtcatc accgtaaagc ccgcgcgagc aagctctctg cccacctcgc gagccaattc
      181 gtagtatgga tgcgactcct tgaagcgcgc cgacccgaac accgtcacgc acgggccgac
      241 gaagtgcaaa cgacgaaacc ccttcacgca ctcggcgaag atccgtgccg cttcgacgaa
      301 ctcggtcccg cggggccgcg gcccctgcaa aaagcgccgc tctgcggggt cgcgagttcc
      361 tttgccccag tcgtctttgg cgacatcgcc cgcgttcggt tcggcatcgt tcattcgtgt
      421 gagttagcac gcctcgcacg gggttcgggg gccaggcgct cagcgcgaac gatccgtgaa
      481 gccgatgccg actcgcaagc tcgtctgcgc gcggtcgtaa ctcccgagcg tctcgccgta
      541 gcccgtgaac agctgaaacc agacgtatgg agccagcttc caggattcgc cgaagagacg
      601 ctgataggtc ggacgccagc gcacatcgag ggttacgctt cccttggcga ggtcgaagtt
      661 cgctcctttc cgcagcgcca ggctcactag tccattcgta tgagcggcga cgtcgtcgaa
      721 cccgaccgcg agcccgagct ccccgtagcc ctgagtctga gtaatgcttt cgttgtccga
      781 gaccccgagc ggataccaga cacgcagctc gacgagccca taaacgtgct tactattgca
      841 agcggcggca acatcgacga acagccggtt ccagctgcgc gaggaatcgc tcgcttcacc
      901 attggactcg tgggcgatgc ctgcttgctc ggagaacaat ccgcacccag gacccggctc
      961 accatgcacc tccgaatgat agtgcgcata gaagagctcg ggagcgtagt tggactcacg
     1021 gaacggcgaa gagcgcgaga agagatccca gagagaccgc tgcgtgtatg cgaagtacac
     1081 cgtgtggtgc cccttgctcg gccaaaggtc gtacttgatg ctgaactgaa acttgacttg
     1141 cgtgtcccgc gtgaaccccg tgatgaagta gtttgcacga tgaaacgtga gcgcgtcgag
     1201 ttcgggctca tcctccggct gcggcgcgtt cgggcttcgg atctcgcctg cccctcccac
     1261 cgctcgtgcg gcaccggggc gctcggaact ttgaccttcg gcgggcgaca ccgtcttcgc
     1321 gctggggtca tcgggcgcag ccggttgcgc gctcgcaagc gagctcgaga acagcgctac
     1381 tacgaatgac agccgtgctc gcatcgtgag tgagcagccg tgacccagcc actctccgga
     1441 cgagcatcca cgccgcgggg ccaacccgtc aacggcgcgt acaccgagcg cagagcaacc
     1501 agagcgaaca gcagcgcctc agcgaggcgg cgttgcgcgc gagctgccct atagtgcaag
     1561 gcctgcgagc gcttaccgcg acgcggcggg gaacgctttg attcgccgag tcagtacacg
     1621 acgcgactgc cgaggttcac gaaccaagcg tcgtcttggt tgcgcgcaac cccgaccacg
     1681 accggaccga cgcggctcca aaggcggtag agaccgccct tcgtgagata tcccgcgccg
     1741 aattcgaagc cgttgctaac gaagaagctt gcccccgcgg tgccgtcgaa cccatgtgcc
     1801 tgcgagtatt cgacgtcggc accgacctgc aggcgatagt aggcagtcga aaaaccggct
     1861 ccgaccgtgc gctcgctgag cttgcgtccg cgcacacgct ccgtcgttcc caagatgttc
     1921 tgtatgccag cgccgagtct aatgttcgtg gtgacgtcgt agacgatgga tgcggcggcg
     1981 tcgacgcggc ctcgattgcg ccaaacggta ttcacctcgt cttcgacgac ggttcgagtc
     2041 ggtacggtcg ttccgccttc cggccgtccg tagaccgtcg tcgtcctcgc aatgtctttc
     2101 gggtcttcta ggtattgcag cgtaagagtc ggcgcgaggg ataccgcgag gcctgggctc
     2161 aactgatacg ccggtgcgag cacgaaggtg tgcgcatggg actgagtatc ggcgaccaac
     2221 gactcgcctt gcccatactg aatcgcgcgc tccttcgagt tcacctgaac gaagtaggcg
     2281 aacccgattc cgaagttgcg cccatccgcc caatccgcca cgaccgagaa ccgatacggc
     2341 tgaattcccc tcacggcgcc gttcgtttga atctccagcc cgtctccgag agcgaccgcg
     2401 gcggggttcg ttgcgatggc gcttcccgcg tacgtccggg gcgcgtacct gaatcccagg
     2461 aacgggaagt cggtcgtccg cgccaaatcg agcggcccgg tttcgtttcc gggctcactc
     2521 gcaatgatcg ggagtcgctc cttgctaatc gagagtactt cttcgtcggc cgccgctccc
     2581 gccgccgccg ggatcaatgc gagcaattgc aagacgtggc tcgcgaagcc cgccgcctgc
     2641 atgttctctt gggcttcggc cttggtcgcg gccttgggat cgatttgatc gcagcgcgcg
     2701 tcgagcatca cgtcttcggg gacaacgaac gcgggaatgc cgtcgagaac attcgtcgga
     2761 gagggtggaa cgaaccaggt gtgctcttcc ttctgctggt tcggccagaa ttcgagggta
     2821 atgttcctcg cacgaatctt gcgcggcctc gggaagatga gccgaaacgt accgtggccg
     2881 cgcgtcgttc tgctcccgat gctcgggtcg tccgcataaa ccacgagcac gtcactcgcc
     2941 agtgttgcac cacgaaccgg gtcttggcac acgaccgtcc ccgccacatc gagccgttgc
     3001 gcgcccgcgc gtgtcgcgaa cgacgcagta ccgacgagca cgctcagagc caaccccaaa
     3061 cactggatcg accgcgaagc ggaattctcg cggggcacca cgaaggatcc ggacttggtg
     3121 ctcggcccgc gcttactttt tctgagcgcc gagctgcaaa tccgcgagtt tgacgtcatg
     3181 cccttccaat tcgaagctgg tcgtcagcgg cgagtaaccg ggcgcgtaaa ccgaaagcga
     3241 aaacggcccg ctgcttccgc tcacggggat ttcgaatgaa ccatcggagt tggtcgagcg
     3301 cgctccgact cccgtcacgc tcactcgcgc cgcggcgact ggtgcaccct gctcaccgag
     3361 gacgcgcccc gtaaccttta ccggcttcac ggttgggagc gaagggagac aatccgcgcc
     3421 tccgttgtag agctgcgagc ccagggtcag cgtgatcacg aacaccgcga cgccgcccgt
     3481 gaccgcgaag cgaatcggat gcttttcggc gcccttccac ggcaattggc cgcgcgccgc
     3541 cgccgtgccc ccgagaaacg aactccctac cgcgaccgtg agcgccaaca ccgcgctgac
     3601 gagaaaacgc gagctacaag gcacctcgtg cccggccgtg ctgagaagaa tgagccccat
     3661 cagaaacagc accgtgaaac cgagaaacgc tgcgccgagc acgagcggcc acaccggttt
     3721 cgaaggctgc gaaagcgagc gtgcggaagc catccttttt ctaagtagcc aaggaagcca
     3781 gcaaatggaa gagcgccttc gcaatcgctg ctcgaacacg gagtccgacg tcgcgcgttc
     3841 ggggagtctc ggttcacgcg acacgtgatc tcgtacgaat cgctcggcga accaatcgct
     3901 ccgttgcatg gcgcgagtgc ggcgatgctc tcaccccatg cggagactga ctacacctcg
     3961 cacgaagcag atcgatcctg tcgggagcgc gtcgggcgcc gacgacttcg agcaaccccg
     4021 tcaccgcgaa ccgaccaggg cggcgtcgtc gatcacgact cccttctcac tccacttcgg
     4081 attcagccgg tgattcgcat tggccgattt gcagcgagcg gctcgttgaa gaacgcttcg
     4141 cggcttgcgg ccattttgca gtgccatggg tcattgccgg cgggggcgcg cgattagtgg
     4201 tgattccgcg cgaagtgccc tatcgtcccg cgaatgaaga ccagcaaagc cgggctgtcc
     4261 gttccgtcgt gtgctcattt agccgcaggg ggcgcggtgc tgcgcggcgc gctggtgttt
     4321 ttggcggcga gcgcgtccgt gggctgcgac tcggacgaga cgcccaaggc gtggaacgca
     4381 ctcgccagct gcatcgtggg caaagacgcg gcgagcccga tcgcggcgcg cgtccaggcg
     4441 ctgaggctcg tccagctcgc aaacgcgagc ggcgacgccg cggcaaagga tggctggccc
     4501 caacggtgca acggatacgc cgacgatctg tttgcgacgt taccgacttc tggcgaaagc
     4561 gcgatcctgc gcggcaaact tcgcgacaag cttggctgca ccgaaaccaa gggtagctgc
     4621 aagctacgaa acgacgcttc cctcctcacc accacgaccg atctcttcga tgcggccaag
     4681 tctagcaacc tcaccacgca agcggcttct ggcgtgcccg caccgaccgc cgcgacccct
     4741 ccctcgctcg acgcgaagag ctggaagtcg ctgagcccaa agccggtcaa agtgtcgggg
     4801 ccaacgctca ccgccaccgg ccgcggcgtc ctggttttga agggcaccga gggccgttcg
     4861 cgtccgagcg gctgcgagtt caccgacggc ttcacgaaag tgcgctgcat cgaggccaac
     4921 tcgagcgtcc cggaacttcc cgtgcagagc atcgaagtcg tgagcgaccc gaaggggatc
     4981 ttcgcggcgg gcctcaccga cgatggcctc gtcgcctacg acttgcagac cggcgaaaag
     5041 tcagaggtcc ggggccgcag cggacacttg ctgcgcgacg gcgtggtcgt cgaaaagtca
     5101 gccaaagaag acgtcagcgc cgagcccccc cgcggcctcc cggggcgcga tgcgaaaggc
     5161 gggaaagccg ccaaagccaa acccaaggcg aagccgaagg gtcccgtgaa ggaggaaggc
     5221 tacgaggcca tcgagctcac cgcgggcaaa gcttcgaaac caatcaaact gccgatcaaa
     5281 gcgagcgtcg gcgatccgat cgctctcggc gatcaaatcg tgttcctcag ccccctcgag
     5341 agcggagccg agctcgggac gttcacggtt tcgcacgcgc gtttgaagcc aggcccggcc
     5401 atcaaaggct ccttctccgg tgccctccac ggctgccaag ccggaagcgc tctcgcggtc
     5461 gccgcgtacg gtccgcgctc cggccaaaac aatgcaaagc caactgccgg cgacggcaag
     5521 acccaattga gcgtgagcct cttccgcggc tctggctggt caaaagcgct cgaaaccacg
     5581 ctgccgttcg accgatcgtt cgagtcggat ctcgtctgta ccaaggacgg cgccacgctc
     5641 gtttgggttc gcaacgcgga aggctccgtt caggtcggcc gcgtcgattg cacggccgag
     5701 ggctgcaagt cgagcgacgt gaagctaccc ggcgtcgaga gcaagtggtg gtggggcgtt
     5761 ggcccgctcg gtgacaaaac cttcctgctc tggcgcagca ccctgggaga aacgcgtctc
     5821 cgcgttgctc cactcgcgga gctcgcgaac gccaaagacg tgctcgcctt cgacaccccc
     5881 gacttcggtg gccctaccgc gggcgaggtc acgtcgctcc tcaccgcgga cgcggctctg
     5941 ctcacctttc gcggcgagca acccgttgcg ctccgcatcg gcaacgacgg agcgctgcgc
     6001 ttgatttcgc cctgagcgcg cggatccaac gaactcgacg gcgccccgct cgtgaagcga
     6061 gccgctcgca aagacgagcg cgtttcacga gctcggcgcg ttcgccgcgc cgttcgttgg
     6121 cgagatcggc cactccctac cgctgcgggg gtcgaagagc aggctatgag tcttgtaaac
     6181 gcgcggagaa cggtacaaga gccgtaacgc gcgaccgagc ggcaacgatc tgtactgctt
     6241 gggccagcgc gcgacgagca cgtcgtggac cctgcgcgcg tggcgcgcgc tcatcgccgg
     6301 gaacaagtga tgctccacgt ggaaaccgaa acccaaggtg agccactcga gccaacgcgg
     6361 taatgtcacg ctgagcgagt tcgcgagcgg gtcgttcacg tccgtgagcg ggctgatgct
     6421 gtggttcgtg aaaatgaagc tcatgacgat cgcgttcgcg acgagcagcg gcacgacgaa
     6481 gcaaaacaag aaagccgcgg gcccgacgat gaacccgagc cccgtccaaa acgcgaccgc
     6541 gagcagggtc ccgcgaaaag ccttgagctg ttcgcggcgc gacaagattc cgagggtctc
     6601 cgctccgaag agcatgtgcg tactctgcac ggagaacc
//
LOCUS       DASTKS010000953         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_598873, whole genome shotgun sequence.
ACCESSION   DASTKS010000953 DASTKS010000000
VERSION     DASTKS010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598873"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFQ35_20090"
     CDS             <1..320
                     /locus_tag="VFQ35_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933019.1"
                     /translation="SCEAAAAAANETMEIGARRGAPDITRDAFARVLENGAYLSACAI
                     PPSAALEICAAVQDGKVVGLSVSSEPRSSAVNACVRRAVARLRFPRSTRLDVTRTHFA
                     PAR"
     gene            complement(330..833)
                     /locus_tag="VFQ35_20095"
     CDS             complement(330..833)
                     /locus_tag="VFQ35_20095"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9933020.1"
                     /translation="MMKEHFVMMARYNTWSNSRVYRMARELSDEQYRRNVGAYFKSLH
                     GTLNHLLVADRIWMWRLTQVGDAPTKLNAVLFDDLSELTEARRAEDERIETFVGNLTD
                     AQMEESVEYRAGSGSQQQKLREVLAHVFNHQTHHRGQAHGILTALGVEEPDSLDLIRM
                     VRERAAR"
     gene            complement(898..2553)
                     /locus_tag="VFQ35_20100"
     CDS             complement(898..2553)
                     /locus_tag="VFQ35_20100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9933021.1"
                     /translation="MSSVGLESVRIVPLGGLGEIGMNCLCIEQDDGILVVDCGAGFPE
                     DDRGIDVFHPDFSWLLERAERVAGIFLTHGHEDHIGGLPYLLAELDVPVWGPPHTLGL
                     VRRRLLEHDFAPHDIDLREAHAGERYTVGPFTVEPVRVAHSIVEASALCIETRAGRVV
                     HSGDFNFDPEPPDGEPTNELRLSELGELGVSLLLSDSTNSDVPERPGSERSVAEAVER
                     LVATAEQRVVVALFASNIQRLITFGEFAQRLGRKICLFGRSLETQYAIASQIGRVHWP
                     SDLLVSQDQAKLVPRERLLVLAGGTQAEKNSALRRLSLGTHQALSLERGDTVILSSRA
                     IPGNERPVNAMINDLLRAGVEVHSRVTDPAVHTSGHAGQSEQSRMIELIRPRCFVPLH
                     GTLHHMMRHEKLAHGLGFTNTLVVENGTPIVCDGHSLQREAPVKFGKVAIAMGGEPMT
                     DATLQSRVELSRSGVAVLSLVLDRYGALAATPGVITRGVPAVDDDPSALRAVAAEVAR
                     AASSFREGRSLGFDEFVKRVARRKLEALSGTRPVIELSVLELD"
     gene            2685..3116
                     /locus_tag="VFQ35_20105"
     CDS             2685..3116
                     /locus_tag="VFQ35_20105"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9933022.1"
                     /translation="MTIKSATPEQAHELVSQGAVYIDVRSEQEFEQGHPPGALNVPIA
                     HFGPAGMTPNPDFLKVMQAAFARDAKLVVGCKAGGRSRKAVEALEAAGFSDLTDMTAG
                     WDGSRDAFGRPLPGWSKRSLPVEAGLPQGQRYEDVKTRKAG"
     gene            complement(3128..3769)
                     /locus_tag="VFQ35_20110"
     CDS             complement(3128..3769)
                     /locus_tag="VFQ35_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933023.1"
                     /translation="MAIDLASVSSAAAPSKPARARRFGPLSWTLLAVAIALLGWFLVA
                     WLRYPSDRTPEGAYLRVMSAVNRGRAQDFFAYTETRAQHACFTVRDYRKKSRERVLAA
                     YPEPERSRLAASYADEAGAADGSDLFALYAKRRGWLGQLRRDMSGIARVERRGERASV
                     ETVRGTRYPFRVREENGIWGLTLFTAELEGEAERAARDFELIDKAASDYERAK"
     gene            3870..>5293
                     /locus_tag="VFQ35_20115"
     CDS             3870..>5293
                     /locus_tag="VFQ35_20115"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9933024.1"
                     /translation="MISAAPSRLDVPAPREDSLRRLLRYAGRHRRTMLLASLCSVLNK
                     LFDIAPEILIGVAVDTVVRREQSTLARFGLTDVKEQVLLLGGLTLVIWMCESLFEFFY
                     GVLWRNLAQTVQHELRSDAYGHVQKLEMSYFENQSSGNLVTILNDDINQLERFLDGGA
                     NSLIQVATSTLLVGAVFFYLAPSIAALAMLPIPVILLGAFYYRRHAEPLYAGVRAQAS
                     AVGARLANNLAGIATIKSYVAEERELAALIAESRAYTEANARAIRVSSAFVPLIRMAV
                     LVGFVATLVIGGYKTVDHTLEVGSYSVLVFLTQRLLWPLTNLAQTVDLYQRAMASTRR
                     VLDLLETNRRDDHGGRPLAKSDVRGRVQFRNVDFAYEGRSRVLEQVSFEVPAGTSAAF
                     IGTTGSGKSTLVKLLLRFYEPTGGDILLDERKLSDIEVSDLRRAIAFVSQDVFLIDGT
                     VAENIAYGSDVRDDAKIREAARLA"
BASE COUNT          930 a         1721 c         1745 g          897 t
ORIGIN      
        1 cttcgtgcga ggcggctgcc gcggcagcca atgaaacgat ggagattggt gcgaggcggg
       61 gggcgcccga catcacgcga gacgccttcg cccgagtcct cgagaacggc gcctacctca
      121 gcgcttgtgc gatcccaccg agcgcagctc tcgagatctg cgcggcagtg caagacggaa
      181 aagtcgtcgg cctgagtgtc tcgagcgagc cgcgcagctc ggccgtcaac gcttgcgtgc
      241 gtcgcgcggt tgcgcggctc cggttccctc ggagcacgag actcgacgtc acgcggacgc
      301 acttcgcgcc ggcacgttga cgcacgggct cagcgcgcgg ctcgctcccg aaccatgcgg
      361 attaagtcca gcgagtccgg ctcctctacg ccgagcgccg tcaagatgcc gtgagcttgg
      421 ccgcgatggt gggtctgatg gttgaaaacg tgcgccaaca cctcgcgaag cttctgctgc
      481 tgtgagccgc tccccgcacg atactcgacg ctctcttcca tctgcgcgtc ggttagattc
      541 cccacgaacg tttcgatgcg ctcgtcttcc gcgcggcgcg cttcggtgag ttcgctcaaa
      601 tcgtcgaaca gcaccgcgtt caacttcgtc ggagcatccc ccacctgcgt caaacgccac
      661 atccaaatcc ggtccgcgac cagaagatgg ttgagcgtgc cgtgaaggct cttgaagtag
      721 gcacccacat tgcggcggta ctgctcgtcc gacaattcgc gcgccatccg gtaaacgcgc
      781 gagttcgacc aagtgttgta ccgggccatc atcacgaagt gctctttcat catgaaacct
      841 ctcgagcggg gagatctacg aacggcttcg cacgaggctc ctcggtgggg gcggcggcta
      901 atccagctcg agcacggaga gctcgatgac gggacgagtg ccggagagcg cctcgagctt
      961 gcggcgagcg acgcgcttga cgaattcgtc gaagccgagc gatcgccctt cacggaaaga
     1021 gcttgccgcg cgagcgactt cggccgcaac cgcgcgcaag gcactggggt catcgtcgac
     1081 ggcaggtacg ccgcgagtga tgactccggg cgttgcggcg agagcgccgt aacgatcgag
     1141 caccagagag aggaccgcga cgccagagcg cgagagttcg acgcgcgatt gcagcgtcgc
     1201 gtcggtcatg ggctcaccgc ccatggcgat ggcgaccttc ccgaacttaa cgggggcttc
     1261 acgttggagc gagtggccgt cgcagacgat gggcgtgccg ttctcgacga ccaaggtgtt
     1321 ggtgaagccg aggccgtggg cgagcttttc gtggcgcatc atgtgatgca gggtgccgtg
     1381 cagtggcacg aagcagcgcg gccgaatgag ctcgatcatg cgcgactgct cgctttgacc
     1441 ggcgtggccg ctcgtgtgaa cggcggggtc ggtgacgcgg ctgtgcacct cgaccccggc
     1501 gcgcaagagg tcgttgatca tggcgttgac cgggcgctcg ttgcccggga tggcgcggct
     1561 cgagagaatg acggtgtcgc cgcgctcgag cgacagggcc tgatgcgtgc cgagcgacag
     1621 ccgacgtagc gccgagttct tctccgcttg agttccgccc gcgagcacca ggagacgctc
     1681 acgcgggacg agctttgctt gatcttggct caccaagaga tcgctcggcc agtggacacg
     1741 accgatttgc gacgcgatgg cgtactgcgt ttcgaggctc ctgccgaaga ggcagatttt
     1801 gcgaccgagc cgctgcgcga actcgccgaa ggtgatgagg cgctggatgt tgctcgcgaa
     1861 gagcgcgacg acgacgcgct gctcggcggt cgcgacgagc cgctcgaccg cttccgcaac
     1921 ggagcgctcc gaacctggcc gctccgggac gtcgctgttg gtgctgtcgc tgagcagaag
     1981 cgaaacgccg agctcaccga gctcactcag cctaagctcg ttcgttggct ccccgtcggg
     2041 cggctcggga tcgaagttga aatcgccgga gtgcaccacg cgaccagcgc gcgtctcgat
     2101 acagagcgcg ctggcctcga cgatggagtg ggctacgcga actggctcga ccgtaaaggg
     2161 accaaccgtg tagcgctcgc cggcgtgcgc ctcgcgcaga tcgatgtcgt ggggagcgaa
     2221 gtcatgctcg agcagacgac gccgcacgag gccgagcgta tgcggcggcc cccaaacggg
     2281 gacgtcgagc tcggcgagca ggtatggcag ccccccgatg tggtcttcgt gaccatgcgt
     2341 caaaaagatg ccagccacgc gctcggctct ttcgagcaac cacgagaaat cggggtggaa
     2401 gacgtcgatg ccgcgatcgt cctctgggaa gcccgcgccg cagtccacga ccaggatgcc
     2461 gtcgtcctgc tctatgcaca ggcaattcat cccgatttcg ccgagtcccc cgaggggcac
     2521 gatgcgaacg ctttccaaac cgaccgatga caacccgcgc ttcttaccac gccttgcgct
     2581 ccgaccgcgc caccatggaa gaacccccga gaagaagccg caggaacgct cttttgcctc
     2641 attgtcagag cgggtcggcg gtgataggcc tcccgccgaa agccatgacc atcaagagcg
     2701 caacgccgga acaggcacac gagctagtca gtcagggtgc tgtctatatc gacgtgcgca
     2761 gcgagcagga gttcgagcag ggccacccgc ccggcgcgct caatgttcca atcgctcact
     2821 tcggccccgc ggggatgacc cccaacccgg acttcttgaa ggtgatgcaa gcggccttcg
     2881 cgcgggacgc caagctcgtg gtcggctgca aagcgggggg ccgctcccgc aaggccgtcg
     2941 aggcgctcga agccgcgggc ttctcggacc tcaccgacat gaccgccggc tgggacggtt
     3001 ctcgggacgc tttcggccgt ccgttgcctg gctggagcaa gcgctcgctg ccggtcgagg
     3061 cggggctccc gcagggccag cgttacgaag acgtgaaaac gcgcaaagcc ggctgagtta
     3121 gcccgagcta cttggcgcgt tcgtagtcgc tcgcggcttt atcgatgagc tcgaagtctc
     3181 gcgcggcgcg ctcggcctcg ccttcgagct cggccgtaaa cagcgtcagc ccccaaatcc
     3241 cgttctcttc tcggacccga aacgggtagc gggtcccgcg taccgtctcg acactggcgc
     3301 gctccccgcg tcgttcgacg cgcgcgatgc cagacatgtc gcgccgaagc tgcccgagcc
     3361 agccgcgccg cttggcgtac agcgcaaaga gatccgaacc gtccgccgcg ccagcttcgt
     3421 ccgcgtacga ggcggcgagc cggctgcgct ccggctccgg atacgcggcg aggacgcgct
     3481 cgcggctctt ttttcggtag tcgcggaccg tgaagcaggc gtgctgagct cgcgtctcgg
     3541 tgtacgcgaa gaagtcctgg gcgcggcccc ggttcacggc gctcattacg cggaggtaag
     3601 cgccttccgg cgtgcggtcg ctcggatatc ggagccaagc cacgaggaac cagccgagca
     3661 gggcgatcgc cacggcgagt agcgtccacg aaagcgggcc aaagcgccgg gcgcgcgccg
     3721 gtttcgaagg cgcagccgct gagctgaccg aggcgaggtc gatagccaac ggcgcgactc
     3781 tactcggtgg gcacgggctc gcaagcggcc tacgcggcgg acgaggtgaa atgggcgcgc
     3841 gcgccaaaca tgacacaatg cgccgcgaca tgatttcggc agcgccttcg cgcctcgatg
     3901 tgcccgcccc gcgggaggac tctcttcgcc gcctactgcg ctacgccggt cggcatcggc
     3961 gcacgatgct gctcgccagc ctgtgctcgg tgctgaacaa gctcttcgac atcgccccgg
     4021 aaatcctcat cggcgtcgcg gtcgatacgg tggtgcggcg agaacaatcg acgctcgccc
     4081 gcttcgggct caccgacgtg aaggagcagg tgctgctctt gggcgggctg actctcgtca
     4141 tttggatgtg cgagtcgctg ttcgagttct tctacggcgt cttgtggcgg aacctcgcgc
     4201 aaacggtgca gcacgagctc cgcagcgacg cctacggcca cgtgcagaag ctcgagatgt
     4261 cgtacttcga gaaccagagc agcggcaatc tggtcacgat tttgaacgat gacatcaatc
     4321 agctcgaacg cttcttggat gggggcgcaa actcgttgat ccaggtggca acgtcgacgc
     4381 tactcgttgg cgccgtgttc ttctatctcg cgccgagcat tgccgcgctc gcgatgctgc
     4441 ccatcccggt catcctcctc ggagctttct actatcgtcg gcacgccgag ccgctctacg
     4501 cgggcgtacg cgcgcaagcg agcgcagtgg gcgcccgcct cgccaataac ctcgcaggga
     4561 tcgcgacgat caagagctac gtggcagagg agcgcgagct cgcagcgctc atcgccgaga
     4621 gccgcgcgta caccgaagcg aacgcgcgcg ccattcgcgt ctcttccgcg ttcgtgccgc
     4681 tcatacgcat ggccgtcttg gttgggttcg tggcaacgct cgtgatcggc ggttacaaga
     4741 cggtggatca cacgctcgag gtcggctcgt acagcgtgct cgtgttcttg acccagcggc
     4801 ttttatggcc gctcacgaac ctcgcgcaga cggtggattt gtaccaacgc gcgatggcat
     4861 ccacacggag agtgctcgat ttgctcgaga cgaatcgccg cgacgaccac ggggggcggc
     4921 cactcgcgaa aagcgacgtg cgcggtcgcg tgcagtttcg caatgtcgac ttcgcttacg
     4981 aagggcgttc gcgcgtgctc gaacaggtga gcttcgaggt acctgccggc acgtcggcgg
     5041 cgttcatcgg caccaccggc tccggcaaga gcacgctcgt aaagctcttg ttacgcttct
     5101 acgaaccgac cggcggcgac atcttgctcg acgaacgcaa gctcagcgac atcgaagtct
     5161 ccgacttgcg gcgcgccatc gccttcgtta gccaagacgt cttcttgatc gacggcaccg
     5221 tggccgagaa catcgcgtac ggcagcgacg tgcgggacga cgcgaagatt cgcgaagcgg
     5281 cgcggctcgc cga
//
LOCUS       DASTKS010000954         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_375421, whole genome shotgun sequence.
ACCESSION   DASTKS010000954 DASTKS010000000
VERSION     DASTKS010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375421"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1403)
                     /locus_tag="VFQ35_20120"
     CDS             complement(138..1403)
                     /locus_tag="VFQ35_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933025.1"
                     /translation="MGENARIEALISVLDRRKGRLFVAFIIAVLLHFPLTPAMPLLRL
                     ANRWAKTKPEPPPPPQLVDVELKEALKSEERRIQPKPEEPPKSAGVQVEPPASVKFAA
                     GSTPKPEDKAEDKPKEPKKEKVKAVGLEGDLSKKLLGKPPVTLGLWLSSLRENPLGAR
                     LREILACDREWKGFLDQGVDVMRDFDGMLVVGPSLFDSSQLTAAVRHDLPPERVHEIM
                     KTLVEGSGDRGSWLMPDVASARIGRSQRVLLPQARDVFFVTPTKGWEALKKTKEPLRV
                     PSAAGRTASLVLVNPNRSLGRIGLTLPKRIQELRLEVFANSDESVDLKVELEAASADA
                     AREEEKRVSAQMRDFFSDVWTAASALGAITGTGSKAHLELPPRLELERDEATLSGMAH
                     LSPNQARATLNLLASFSCRRKGSKAASSK"
     gene            1546..2595
                     /locus_tag="VFQ35_20125"
     CDS             1546..2595
                     /locus_tag="VFQ35_20125"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9933026.1"
                     /translation="MQRRSFLGASAAVGAAVLVGCHRASPRERVMSAIARTVSDGVTE
                     MLAQTKSLEDATSALEQNASAALVARSQELFRQTAILWKRVGTFRDGPFQEQNALLRV
                     TRYPARARVIEAVLAESRPLDAGRVAELGSGEKGLYAIEYLLFDSSTETASSRLLGAK
                     GPRARALLAGLSREVTSLGDKIAVVLGRRGTRLNEELLQNPQRGLGRLVNTLIEGLEV
                     MAEAQLGMIYRLSEVNHLDRASVEGFASGISHRLALARLEGTERLYRGGHDNVSELVR
                     SASPNADASVRERLDNALASLRGLKMPLEEVAQRERLRLQEAYLKVKSLETGMKVHLP
                     SALGISITFSSTDAD"
     gene            2734..3780
                     /locus_tag="VFQ35_20130"
     CDS             2734..3780
                     /locus_tag="VFQ35_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933027.1"
                     /translation="MLSLLRAPEANAAHWPVRAALRRQLALPLLGALLALACSEEVPL
                     GSWGKGGGGSGAGGGSGGITSGGSTSEAGSSGEGNGACTRTGTPGPVNMSGSEVGATL
                     TSSDWSWSARADAMEIDLTVEDEPPPGDDGYFWAYQFGLAGSPGMAGGFIGLQVNGKF
                     TNTETNVTDYSTRLAVFWIAGPPLDGKFEDIVEPRAVRYSTPAFGTQWLAMNVIYPWE
                     KCRTYRLRVGRHSVENGDIWYGAWITDTTTQVTTFIGRILVPGSWGQLARTSTSWCDR
                     IGWGSVNSCSALSHVSASWRSPTATVMATNTTFMPSASAKNSFGSSTSPLRCASSRFT
                     DLPDGVRQESGVSP"
     gene            3958..>4567
                     /locus_tag="VFQ35_20135"
     CDS             3958..>4567
                     /locus_tag="VFQ35_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9933028.1"
                     /translation="MKNVAIAVFAFPLLLGLFNCGGEKEKSSSNGGSASGGAASGGAA
                     SGGMSISRGGSVVVGDEIVNGLGALPVPPGDGGVAKPSGTPGNLKVLDWAGFKSAVSY
                     TFDDTNSSQIAHYAELQALGVHYTFYLITGKTEINDPTWPHALEDGHELGNHTRSHNK
                     MATDEDIDAASDLLAEKFHVTTWTMAAPYGDGSYVSLATPRFL"
BASE COUNT          822 a         1456 c         1510 g          779 t
ORIGIN      
        1 cgcgcagcag cgcggcgggc aatggctcga agcgaggaag acaacgtcaa cacgactccc
       61 cgaatatcac gctcgttcgc gccgagcaac gattcgatcg caggcggtgt gtgcggcgcc
      121 ctcgagtaga ggggctctca cttcgagctc gcggccttcg atcccttgcg acggcagctg
      181 aatgaagcga gcaagttgag ggtggcacgc gcctggttcg gcgaaaggtg cgccataccg
      241 ctcagcgtcg cctcgtcgcg ctcgagttcg agccgcggtg gcaattcgag atgagccttg
      301 gatccggtgc cggtgatcgc gccgagcgcc gaggccgcgg tccacacatc ggagaagaag
      361 tcgcgcatct gcgccgaaac gcgcttttct tcttcccgcg cggcgtccgc gctggcggct
      421 tcgagctcga ctttgaggtc gaccgactcg tcgctgttcg cgaagacttc gaggcgcaac
      481 tcttggatgc gcttcggcag cgtcaagccg atgcggccga gcgagcggtt cggattgacg
      541 agcacgaggc tcgcggtgcg accggctgcg ctcgggacgc gcaaaggctc tttcgttttc
      601 ttgagcgcct cccaaccctt ggtcggggtg acgaagaaga cgtcacgcgc ctgcggcaac
      661 agtacgcgct ggcttcgacc gatacgcgcc gaagccacgt cgggcatgag ccagctgcca
      721 cgatcgccgc tgccctcgac caacgtcttc atgatttcgt gcacgcgttc gggaggcaag
      781 tcgtgtcgaa ccgcggccgt gagctgggag gaatcgaaca ggctcggtcc gacgacgagc
      841 atgccgtcga agtcgcgcat cacgtcgacg ccctgatcga gaaagccctt ccattcacga
      901 tcacaggcga ggatttcgcg gagccgcgca ccgagcggat tttcgcgaag actgctcagc
      961 caaagtccga gcgtcaccgg tggcttgccg agaagtttct tggacaggtc tccctccagg
     1021 ccgaccgcct tcaccttctc ttttttgggc tccttgggct tgtcctctgc tttgtcttcc
     1081 ggcttcggag tggagccggc tgcgaatttg acgctcgccg gcggctcgac ttggacgccc
     1141 gcggactttg gaggctcctc gggcttcggc tgaatacgcc gctcctcgct cttcagcgct
     1201 tccttcagct cgacgtcgac gagctgcggg ggcggcggcg gctcgggctt cgtcttcgcc
     1261 cagcgattcg ctagccgaag cagaggcatg gccggcgtga gcgggaagtg cagcaaaaca
     1321 gcgatgatga aggccacgaa gagtcggccc ttccgtcgat ccaggacaga aatcagtgcc
     1381 tcgatacgcg cgttctcgcc caagctggct ctggctagca aagaaggtat gcacgggcca
     1441 gccctcggcg gcagaaggcc cggcgcaccc ggccctcgtg agggaatacc cgtgggtttc
     1501 tcaggtgaaa ctctcggacg gaacagctac atctcgcgtt cgctcatgca acgaaggtcc
     1561 tttctcggag caagcgcagc cgtcggcgcc gcggtgctcg tcggctgcca ccgagcgagc
     1621 ccgcgcgaac gagtgatgag cgccattgct cgcacggtga gcgacggcgt caccgagatg
     1681 ctggcgcaaa cgaagtcgct cgaggacgcc acgagcgcgc tggagcagaa cgcgagcgcg
     1741 gccctcgtcg cgcgctctca agagctcttc cgccaaaccg cgatactttg gaaacgcgtg
     1801 gggacctttc gggacggtcc cttccaagag cagaatgcgc tgctccgcgt gacgcgctac
     1861 ccggcccgcg cgcgcgtcat cgaagcggtg ctggcggagt cgcggccgct cgacgcgggt
     1921 cgagtcgctg agctcggaag cggcgagaag gggctctacg cgatcgaata cttgctgttc
     1981 gactcgagca cggagaccgc gtcgtcacga ctactgggag caaaagggcc gcgcgcacga
     2041 gcgctgctcg ccgggctgtc gcgcgaagtc acttcgctcg gtgacaagat tgcggtcgtg
     2101 ctcggccgtc gcggaacgcg gctgaacgaa gagctcctgc agaacccgca gcgaggattg
     2161 ggacgcctcg tcaacacgct gatcgaagga ctcgaggtca tggcggaggc gcagctcggc
     2221 atgatctacc gcttgagcga ggtcaaccat ctcgatcgcg cgagcgtcga gggcttcgct
     2281 tcgggcatct cgcaccgcct ggcgctcgct cggctcgaag gcacggagcg cctctatcgc
     2341 ggcggtcacg acaacgtgtc cgagctcgtg cgcagcgcat cgccgaacgc cgacgcttcc
     2401 gtccgcgaac gcctcgacaa cgcgctcgct tcgctccgtg ggttgaagat gccgctcgaa
     2461 gaggtcgcgc aacgcgagcg cctacgcctg caggaggcgt acctcaaggt aaagagcctc
     2521 gagaccggaa tgaaagtgca cctgccgagc gcactcggca tcagcattac gttttcgtcg
     2581 accgacgcgg actgagtcga tcacgctacc ggcgcagcgg gaaacttgcg ccaaactgct
     2641 agaaccggtg agatatttgc gccggccgag acggaagcgg agtcagcggg catgtatctc
     2701 gtaaacttct agccgtttcc cggcccgcct tcgatgctct ccctcctgcg cgcaccagaa
     2761 gccaacgcgg cccattggcc cgtccgcgct gcactgcgcc gacagctcgc tctcccgctc
     2821 ctcggagcac tgcttgcgct cgcgtgcagt gaggaggtgc ctctcgggtc ctgggggaaa
     2881 ggcggcggtg gcagcggcgc gggcggcggc tcaggaggga tcacctcggg cgggagcacg
     2941 agcgaagcgg gctcgagcgg cgaaggcaac ggagcttgca cgcgcaccgg cacgccgggc
     3001 ccggtgaaca tgagtgggtc ggaagtgggc gcgaccctga cctccagcga ctggtcatgg
     3061 agcgctcgcg ccgacgcgat ggaaatagac ctcacggtcg aggacgaacc gccgccggga
     3121 gacgatggct acttctgggc ataccaattc gggctcgcgg gcagccccgg catggccggc
     3181 ggattcatcg ggctgcaggt gaacggaaag ttcacgaaca ccgaaaccaa cgtgaccgac
     3241 tactccactc gcctggcggt gttctggatc gcggggccac cgctcgacgg gaagttcgaa
     3301 gacatcgtcg agccgcgcgc cgtgcgctac agcacgcctg cgttcggcac gcagtggctg
     3361 gccatgaacg tgatttatcc gtgggagaag tgccgcactt atcggctccg cgtggggcga
     3421 cactcggtcg agaacggcga catctggtac ggcgcgtgga tcactgacac caccacgcaa
     3481 gtcaccacct tcattggacg cattttggta cccggaagct gggggcaact cgcacgcacg
     3541 agtacgtcct ggtgtgatcg catcggatgg ggatccgtca actcttgcag cgcgctctcg
     3601 cacgtctcgg cgtcgtggcg gtcgccgacg gctacggtca tggcgaccaa cacgaccttc
     3661 atgccgtccg cgagcgcgaa gaacagcttt ggctccagca cctcgccgct gcgttgcgcg
     3721 tcgtcgcgct tcaccgattt gccggacggt gttcgccagg aaagcggcgt ttccccatga
     3781 aacggtgggc ctgtgggcct gtgggcctct ccggacgagc ccaatactga gccgattccg
     3841 gctagtcgcg ggcactgtcc ggcgagtacc aaagccccgg aacttcgttc ctcagtcgga
     3901 ccaaggtact aaactgcggc ggagagcgcc tccgcctcga gcgcgtggag acaattcgtg
     3961 aagaacgttg cgatcgcggt gttcgctttt ccgctccttt tgggcttgtt caattgcggc
     4021 ggcgagaagg aaaaatcttc atccaacggc ggctccgcga gtggcggtgc ggcgagcggc
     4081 ggtgcggcga gtggtggcat gagcatcagc agaggaggct cggtggtcgt aggagacgag
     4141 atcgtcaacg ggttaggagc tctgccggtg ccgccgggcg acggcggggt tgctaaaccg
     4201 agcgggaccc cgggcaacct caaggtgctc gattgggcgg gcttcaagtc tgcggtgtcc
     4261 tacacgttcg acgacacgaa ctcctcgcag atcgctcact acgccgagct gcaagcgctc
     4321 ggcgttcact acacgttcta cctcatcacc ggcaagacgg agatcaacga tccgacttgg
     4381 ccgcacgcgc tcgaggacgg gcacgagctc ggcaatcaca cgcggagcca caacaagatg
     4441 gcgaccgacg aagacatcga tgcggcgagc gatctcctcg cggagaagtt ccatgtgacg
     4501 acgtggacca tggccgctcc ctacggagac ggaagttacg tttcgctcgc aacgcctcgc
     4561 ttcctcc
//
LOCUS       DASTKS010000955         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_153381, whole genome shotgun sequence.
ACCESSION   DASTKS010000955 DASTKS010000000
VERSION     DASTKS010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153381"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFQ35_20140"
     CDS             complement(<1..584)
                     /locus_tag="VFQ35_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9933029.1"
                     /translation="MSLSCACNAKKGSAPAAPSATASAAPSSAPISTVVVDHGELDTK
                     APTNAQRIAATAIATTVYKLPDTGSRRLGYIRLGGSVQRDAEPVAGRGCKGKFYKVYP
                     MGYVCTDEASADMEAPIVRAASRRPDLSKPLPYKYGFVRATSPLYLRVPSRAEQMKSE
                     FKLEEHLSWYKEHKSEVQGVVAGANDIPLDPRGFP"
     gene            729..2138
                     /gene="miaB"
                     /locus_tag="VFQ35_20145"
     CDS             729..2138
                     /gene="miaB"
                     /locus_tag="VFQ35_20145"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9933030.1"
                     /translation="MPTYALVTFGCQMNQHDSERIAEVLREVGYSECGAPEHADLVLL
                     NTCSVREKAEQKLRSEVGRLGMLKELRPDLVIGVAGCVAQQEGERLIKRMPQIDLVIG
                     PDRIADLPRVLADLELGSPPQVRVGFDLEEPRFLTASPLPGKAPVSAFVTVMKGCDER
                     CSFCIVPTTRGPERYRPAREIVAEIARLVSAGTLEVTLLGQTVNSYRDPERSLTVATS
                     EGEDESEFAWLLRRIVAEVPGLLRLRYTSPHPRHLTRALVAAHAELATLVRHVHLPVQ
                     SGSDRVLKRMIRRYTAADYRERVARLREAVPGVTLSTDVIVGFPGETREDFQATLELV
                     RDIEFTGLFGFKYSQRPNTPALRFADDVSEEEKSERLAELFDVADAYRRAHLESRVGL
                     VEDVLVEGRGRGDEYSGRSERNEIVHIGSAESDPSGQLVKVKIERAYKNSLFGTLAEP
                     PAAPAREPRTRSRQLPLLG"
     gene            2151..>2294
                     /locus_tag="VFQ35_20150"
     CDS             2151..>2294
                     /locus_tag="VFQ35_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9933031.1"
                     /translation="MPALVRGFSGRSPRLGERVYLAETAVVVGDVELGDDVSIWFGAV
                     LRGD"
BASE COUNT          359 a          757 c          788 g          390 t
ORIGIN      
        1 ggaaagccgc gtggatcgag cgggatgtcg ttcgctccgg cgacgacccc ctgcacctcg
       61 cttttgtgct ccttgtacca gctgaggtgc tcctcgagct tgaactcgct cttcatctgc
      121 tcggcgcggc tcggaactcg caggtagagc ggcgacgtcg cgcgcacgaa gccatacttg
      181 tacggcaacg gcttcgacag gtcggggcga cgactggccg cgcgcactat cggcgcttcc
      241 atatcggcgg aggcttcgtc ggtgcagacg tagcccatcg ggtagacctt gtagaacttg
      301 cccttgcagc cgcgcccggc gacgggctcg gcgtcgcgct gcacgctgcc gccgagtcgg
      361 atgtagccga gccttcgcga gccggtgtcc ggcagcttgt acacggtggt cgcgatcgcg
      421 gtcgcggcga tgcgctgggc gttggtcggg gccttcgtgt cgagctcacc gtgatcgacg
      481 acgacggtcg agatcggcgc gctactcgga gccgcgctcg cggtcgcgct cggggccgcc
      541 ggtgccgagc ccttcttggc gttgcaggcg cacgacaacg acaccagaac ggccgtagtg
      601 gctcgcgagc ggcgggttcg ggtcagcacg ccgcccgtca tatcgcggcc ggtttcgaga
      661 tcaaggcggg aaccggtgac ttcgtgccaa accgcgcccg agaggctagg ctccggcccg
      721 gaagaccgat gccgacctac gctctcgtca cttttggctg ccaaatgaac cagcacgact
      781 cggagcgcat tgccgaggtg cttcgcgagg tcggctattc agagtgcggc gcgccggagc
      841 atgccgatct ggtgctgctc aatacttgct ctgtccgcga aaaagccgag cagaagctgc
      901 ggagcgaggt gggtcgcctc ggcatgttga aggagttgcg cccggacctc gtcattggcg
      961 tcgccggttg cgttgcgcag caagagggcg agcggctcat caagcgcatg ccccaaatcg
     1021 atctggtgat cggacccgac cggatcgccg atttgccgcg cgtgctcgcc gacttggagc
     1081 tcggctcacc tcctcaagtg cgggtcggat tcgacctcga agagcctcga tttttgacgg
     1141 cgagtcccct ccccggcaag gctccggttt cggcgttcgt gaccgtgatg aagggctgtg
     1201 acgagcgctg ctcgttctgc atcgtcccga ccacgcgcgg gcccgagcgc taccgccccg
     1261 cgcgcgagat cgtcgccgag atcgcgcggc tcgtctctgc gggcacgctc gaggtgaccc
     1321 tgctcggtca gaccgtgaac agctaccgcg acccggaacg ttcgctgacg gtcgcaacga
     1381 gcgaaggaga ggacgagagc gagttcgcgt ggctccttcg ccgcatcgtt gcggaggtcc
     1441 cggggctgct ccggctgcgc tacacgagcc ctcacccgcg gcacctcacc cgcgctctcg
     1501 tcgccgcgca cgccgagctc gccaccctgg tcaggcacgt gcacttgcca gtgcagtccg
     1561 gcagcgatcg ggtgttgaag cgcatgatcc gccgctacac ggccgccgac taccgcgagc
     1621 gcgtcgctcg cttgcgcgaa gcggttccgg gggtgacctt gtcgaccgac gtcatcgtcg
     1681 gtttcccggg agagacgcgc gaagacttcc aggcgacact cgagctcgtt cgagacatcg
     1741 agtttacggg tctattcggg ttcaagtatt cgcagcggcc caacacgccc gccctgcgct
     1801 tcgccgacga cgtctccgag gaggaaaaga gcgagcggct cgcggagctc ttcgacgtgg
     1861 ccgacgctta ccgccgggcg cacctcgaga gccgcgtggg gctcgtcgaa gacgtgctgg
     1921 tcgaagggcg cgggcgcggc gacgaataca gcgggcgcag cgagcgcaac gagatcgtgc
     1981 acattggcag cgcggagagc gatccgagcg gccagctcgt gaaggtcaaa atcgagcgcg
     2041 cttacaagaa ctcgctgttc ggcacgttgg cggaaccgcc cgccgcgccc gccagggagc
     2101 cacgaactcg gagccgccaa ctcccgctgc tcggctgagt gaggacaccc atgcccgcgc
     2161 tcgtcagagg tttttcaggt cgctcgccac gcttggggga gcgtgtgtac ttggccgaaa
     2221 ccgcggtcgt cgtgggcgac gtcgagctcg gcgacgacgt gagcatctgg ttcggcgccg
     2281 tgctgcgcgg cgac
//
LOCUS       DASTKS010000956         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1270190, whole genome shotgun sequence.
ACCESSION   DASTKS010000956 DASTKS010000000
VERSION     DASTKS010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270190"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFQ35_20155"
     CDS             complement(<1..1248)
                     /locus_tag="VFQ35_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HET9933032.1"
                     /translation="MGIVDDLREIADRVQKNFEEQRRLLGFQEYLELFATDPVRYSRD
                     AARYLRDAFDYYGKTEITRPWGNLTRFRLFDLPFLDPADARREQLVGQEFVQGEIYRA
                     LSNFVREGRPNRVVVMHGPNGSAKSTVAACLMRALEHYSSTDDGALYRFHWVFPNEKT
                     LRGVIGFGGKRATPLSREESFAHLPEEEIDARLLMEVRDHPLFLLPRPERKAILERMY
                     KESGVTDPPPAWVLRGELSHRSRQVYEALLGTYDGSLEQVLRHVQVERYFISRRYRTG
                     AVTLGPQLSVDAGERQITADRSLGALPSALQAITMFEAFGELVDASGGLLEFSDLLKR
                     PLDAFKYLQITAETGEVSLRSQTLHVNCVMLASANEVHLAAFREHPEFESFRGRFELV
                     RAPYLLSWIDEQSIYDAQIAPHVR"
     gene            complement(1312..1896)
                     /locus_tag="VFQ35_20160"
     CDS             complement(1312..1896)
                     /locus_tag="VFQ35_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933033.1"
                     /translation="MSLSLRSARAGQVVAWVVFASGAVLACNKDRPASVEAPTSGGVP
                     SATPEPVAAAPAAAQASAAEPAAAAPSEPSAASARVDEANFSISITPSGAYAAGKPAE
                     AQIVLDAKPPFHVNDKYPYKFKLKDAAGLTFANPVVGKDNVKLETQRATMTVPFTVKD
                     PGKHTLAGQFSFSVCTEDKCLMEKRDLALSLEAK"
     gene            complement(1952..>2450)
                     /locus_tag="VFQ35_20165"
     CDS             complement(1952..>2450)
                     /locus_tag="VFQ35_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933034.1"
                     /translation="KEPKPPSVAGAGKKYPVPTTLDRVLAQAFKKMAALRTASVGALA
                     DQVGQAYGLTGDHRAWAVTREADLAKQIEEKLPALLAAPVEAPKKTVADDFFGESAAL
                     GEGLDVPGSAAPVVSSVAQTWSSDNDADAIQIPTKPALPLLLLVGGGALVLGVLVVVV
                     LVLIR"
BASE COUNT          415 a          793 c          820 g          422 t
ORIGIN      
        1 gcggacgtgc ggcgctatct gcgcgtcgta gatcgattgc tcgtcaatcc agctgaggag
       61 gtacggggcg cgcacgagct cgaagcggcc acggaagctc tcgaactccg ggtgctcgcg
      121 gaacgccgcg aggtgcacct cgttcgcgct cgccagcatc acgcaattga cgtgcagcgt
      181 ctgcgagcgg agcgacacct cccctgtctc ggcggtgatc tgcaggtact tgaaggcatc
      241 gagcggacgc ttcaacaggt ccgaaaactc gagcaggcca ccgctcgcgt cgacgagctc
      301 gccgaatgcc tcgaacatcg tgatggcttg cagcgcggag ggcagcgccc caaggcttcg
      361 atccgccgtg atctgccgtt ctccggcgtc gaccgagagc tgcggaccga gcgtcacggc
      421 gccggtgcgg tagcgccgtg aaatgaagta gcgctcgacc tggacgtgcc ggagcacctg
      481 ctcgagcgag ccgtcgtacg tgccgagcag cgcctcgtag acctgacggc ttcggtgaga
      541 cagctcaccg cgcagcaccc aggccggagg cggatccgtc acgcccgact ccttgtacat
      601 gcgctcgagg atcgctttgc gctcggggcg cgggagcaag aacaaaggat ggtcgcgcac
      661 ctccatcaag aggcgcgcgt cgatttcctc ctcgggcaag tgtgcgaagc tttcctcgcg
      721 gctgagcggc gtcgcgcgct ttccgccgaa cccaatgacc ccgcgcagag tcttctcgtt
      781 cgggaagacc cagtgaaaac gatagagcgc gccgtcgtcg gtggacgaat agtgctcgag
      841 cgctcgcatg aggcaggccg cgacggtgct cttcgcggag ccgttcggac cgtgcatgac
      901 cacgacacgg ttgggacgcc cctcgcgcac gaaattgctg agcgctcggt agatctcgcc
      961 ctgcacgaac tcttgtccga cgagctgctc gcgccgcgcg tcggcggggt cgaggaacgg
     1021 gaggtcgaac aggcgaaacc gcgttagatt gccccaaggc cgagtgatct cggtcttgcc
     1081 gtagtagtcg aacgcatcgc gcaggtaacg cgcagcgtcg cgcgagtagc ggaccgggtc
     1141 ggtcgcgaat agctccaggt actcctggaa gccgagcagc cggcgttgct cttcgaagtt
     1201 cttctgaacg cggtcggcta tctctcgaag gtcgtcgacg atccccatcg ggcccatcct
     1261 acacgttggc gggcgagcgc gaaccgtgaa ttttccagcg tgctcgcggg cttatttcgc
     1321 ttccagcgaa agcgcaaggt cgcgcttttc catcaggcac ttgtcctcgg tgcagacgga
     1381 gaacgaaaac tgtccggcga gcgtgtgctt gccggggtcc ttcacggtga agggcacggt
     1441 catggtggct cgctgggttt cgagcttcac gttgtctttg ccgacgacgg ggttggcgaa
     1501 cgtgagaccg gccgcgtcct tcagcttgaa cttgtacgga tacttgtcgt tgacgtgaaa
     1561 cggaggcttc gcgtcgagca cgatctgcgc ttctgccggc ttgcccgcag cgtacgcacc
     1621 gctcggagtg atgctgatcg agaagttcgc ttcgtcgacc cgcgccgagg ccgcgctggg
     1681 ctcactcggt gctgcggctg cgggttccgc cgcggaggct tgggcggctg cgggcgcggc
     1741 cgcgacgggc tccggcgttg cgctcggtac cccaccgctc gtcggggcct cgaccgaagc
     1801 aggacgatcc ttgttgcagg cgagtacggc gcccgacgca aagactaccc acgcgacgac
     1861 ctggccggcg cgcgccgaac ggagcgaaag actcatggtg atttgatacc acgagatctt
     1921 cgcgagggta gagccgcgag aaaacgccga ttcagcggat cagcaccagc acgacgacga
     1981 cgagcacacc gaggaccagc gctcccccac ccaccaaaag aaggagcggc aaggccggct
     2041 tcgtcgggat ttgaatcgcg tcggcgtcgt tgtcgctcga ccaagtctga gccaccgagg
     2101 agaccacggg cgcggccgag ccggggacgt cgagcccctc gccgagcgcc gcgctttcgc
     2161 cgaagaagtc gtcggcgacc gtctttttcg gcgcctcgac gggcgcggcc agaagcgcgg
     2221 gcagcttctc ctcgatctgc tttgccaaat cggcttcgcg agtcaccgcc cacgcgcggt
     2281 ggtcgccggt gagcccgtac gcttgaccta cttggtccgc caacgcaccc accgaggcgg
     2341 tgcgcagcgc ggccatcttc ttgaacgctt gcgcgagcac ccgatcgagc gtggtcggga
     2401 cgggatactt cttgcccgcg cccgcgacgc tcggtggctt cggctctttg
//
LOCUS       DASTKS010000957         4048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1716563, whole genome shotgun sequence.
ACCESSION   DASTKS010000957 DASTKS010000000
VERSION     DASTKS010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716563"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..602
                     /locus_tag="VFQ35_20170"
     CDS             285..602
                     /locus_tag="VFQ35_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933035.1"
                     /translation="MQRKSIVIALLVQALAMLGCSAGDVRDPTPGGEQSSETSSEGVV
                     EANQCNGAGNCVPSSNYCCSGLGAVPRGTCSNYCCLRSGAHNAGDARLCCSGKKQSGN
                     YCW"
     gene            complement(764..1186)
                     /locus_tag="VFQ35_20175"
     CDS             complement(764..1186)
                     /locus_tag="VFQ35_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanoglobin"
                     /protein_id="HET9933036.1"
                     /translation="MSAPEGAPRPAPPSPFSEMGGSEAFVRELAKTFYDFMEVNEPEL
                     ARMHRGEQGRVGEEARERFALFLIGWLGGPQTYMERHGHPRLRMRHGHLPVNTRMRDA
                     WLRCMNHALEAHPVPPNVRAFLKQRFSELADFLRNRPE"
     gene            1231..2619
                     /locus_tag="VFQ35_20180"
     CDS             1231..2619
                     /locus_tag="VFQ35_20180"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9933037.1"
                     /translation="MHGGRLALVVAALAAGLGGALLAVRSPLELALSPPLDVSFDGRI
                     LPPRVEVAEWLEERQRAIRDKPVDIDAGGEHLTATFRELGWSLDTRRMLERAQALPRA
                     ETLMGRLQRAFGGPPLLPPELPLRVVVDEARSRAYLEELARRVYRAPRNAARDVVHHE
                     NVHEADGRELALESTLARLETSAELGHAAFELSFRTLPPKVFLRDLPEVDVSRVLGAY
                     ETDFKHHAGARSVNIRRAAALLNGMILQPGERFSFNRRVGPRTLERGFVEAPVLVKDE
                     TEKGPGGGVCQVATTLHAAAVLGGLTMLERRSHSRPSGYAPLGLDATVIDGKVDLRFE
                     NSLDTPLVIHAFLPTPTSIRVELLGHDAPGQVEHTYRVIERHPYFRRIVESSEVARGN
                     YERKQKGSPGYDVVSFVRTTEPNGSVVTRRYKSKYWPVPEVLWVGPGTDVAELPPLPD
                     GSAGLEPTESGG"
     gene            2686..3822
                     /locus_tag="VFQ35_20185"
     CDS             2686..3822
                     /locus_tag="VFQ35_20185"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9933038.1"
                     /translation="MGYLFGALGYAMVVLVLGGAAHGGARLGSRWLTHKRFRWFDAAP
                     PASSLARSTLVRTFSVFAALLVAFGLQVIGLLVSGHAEPTLSVEVIPGPAKRAGLQSG
                     DRIVSVDGKKLTSFEELRGEIQRRPGERRLELDRGGQPLTLLVTPEDGRIGVMPRYRQ
                     LSISLPAALEGAAGAISKYPRTILKSAFSPSERTELAGPVAIVNSVGGQSNGGSRALV
                     LAMIAGVFWPIALGVHLFDALTLTLFRATHGWAASAEVDIARHARIFQTLLVSCLCAL
                     GYVVLAAIQEGADFGNGALPGLIVLGPAAISAVLLVGIAARLRFGVGVAIGFVLACVT
                     IPCAVIVLSGVALHWQRLELRRRGYAVSWFVTLPPSAGPSQGVL"
BASE COUNT          671 a         1314 c         1336 g          727 t
ORIGIN      
        1 aatcgtcggg tgcaaccgct cggctgccag ttcacgggcg gcaatgggcc tagcggttcc
       61 ggcggactcg tcgcatcgat ggcactcggc ttggtgatct ctacacgcaa gcgacgtgag
      121 gcgacgcttg ctcgcgcaag ccgatggcat gcaccttgcg aatgaacgcg gtcgatcaac
      181 acgtcgttga ttacacccaa gctcggttgg gtgaatggaa ccaaggcaac actgagtgac
      241 aggtctggcc aaagcggtca ccggtatagg gaggaactga agtcatgcag aggaagtcga
      301 ttgtcattgc tctactcgtc caagcgctcg cgatgttggg ctgcagcgct ggcgacgtac
      361 gcgaccctac gcctggcggc gagcagtcga gcgaaacgtc atcggaaggt gtcgtcgagg
      421 cgaaccagtg caacggtgcg ggaaactgcg ttccgagttc caactactgc tgcagcgggc
      481 tcggcgcggt tccgcgaggc acctgctcga actactgttg cctacgctcc ggagctcaca
      541 acgcagggga cgcgagactt tgctgctcgg gaaagaagca aagcggcaat tactgctggt
      601 gagagccgaa tcgcaatagc ttgaggcgca ccaacgactg tcggcgcaca caggctcaat
      661 cgcgcgtgct gagccgtgct catttcgagc gcgactcagc gcgtcgcgac ctactcggtg
      721 acggacccgt cgaaacctgt tcgccgcaca aggaacgccg cgttcactcg gggcggttgc
      781 ggaggaaatc ggcgagctcg ctgaaccgct gcttcaaaaa ggcacgaacg ttcggcggaa
      841 cggggtgcgc ctcgagcgcg tggttcatgc aacgaagcca cgcgtcgcgc atgcgagtgt
      901 tcacgggaag gtggccgtgg cgcatacgaa ggcgcgggtg gccatgacgc tccatgtacg
      961 tttggggccc cccgagccac ccgatgagaa agagcgcaaa tcgttcacga gcctcttcgc
     1021 cgacgcgccc ctgctcgccg cggtgcatgc gggcgagctc gggctcgttc acttccatga
     1081 aatcgtaaaa ggttttggcg agctcgcgga cgaaggcttc gctgccgccc atctcggaga
     1141 acggggacgg cggtgcgggc ctcggagctc cttctggggc gctcatgttt cgggcggttc
     1201 ctagcacgct ttggggtaga ctgggacgcg atgcacggtg gtcggttggc gctggtcgtc
     1261 gcggccttgg ccgcaggact cggcggcgca ctgctcgcgg tccgctcgcc cctcgagttg
     1321 gcgttgagcc ctccgctcga cgtctcgttc gatggccgca tcttgccgcc acgcgtcgag
     1381 gtcgccgagt ggttggagga acgacaacgt gcgattcgcg acaagccggt cgacatcgac
     1441 gctgggggag agcacctcac cgccactttt cgagagctcg ggtggtccct cgatacgcgc
     1501 cgcatgctgg agcgcgcgca ggcgttgcct cgcgccgaga cgctcatggg ccgcttgcag
     1561 cgtgctttcg gcggtccgcc gcttctccca ccggagttac cgctacgcgt cgtagtcgac
     1621 gaggcgcgca gtcgcgctta cctcgaagag ctggcgcgac gcgtctaccg tgccccccgg
     1681 aatgcggcgc gtgacgtggt gcaccacgaa aacgtccacg aggccgacgg ccgcgagctc
     1741 gctctcgaat cgacgttggc ccgcctcgag acgagcgcgg agctcgggca tgccgccttc
     1801 gagctctcgt tccgaacgct accgccgaag gtcttcttga gggatctgcc ggaggtcgac
     1861 gtctcgcgag tgcttggcgc gtacgagacc gatttcaaac accacgccgg cgcccgctcc
     1921 gtgaacatac gacgcgcagc cgcgttgcta aacggcatga ttctccagcc aggcgagcga
     1981 ttcagcttca atcgccgggt gggcccccgc acgctcgagc gcgggttcgt cgaggcgccg
     2041 gtgctcgtca aggacgaaac tgaaaagggg cctggcggcg gcgtctgtca ggtcgcgacg
     2101 acactgcacg ccgcggcggt gttgggcggc ctcacgatgc tcgagcgccg gagccatagt
     2161 cggccgagcg gttacgcccc gctcgggctc gacgcgaccg tcatcgacgg caaggtcgac
     2221 ttgcgcttcg aaaactcgct cgacaccccg ctcgtcattc acgccttcct accgactcca
     2281 acctcaatcc gagtcgagct actgggccac gacgcgccgg gccaggtcga acacacatac
     2341 cgtgtgatcg agcgccatcc ctacttccgc cgcatcgtcg agagttcaga ggtggcgcgc
     2401 ggcaactacg agcgcaagca aaagggcagt cccggatacg acgtagtgag cttcgtccgc
     2461 acgaccgagc cgaacggcag cgtcgtgact cgtcgctaca aaagcaagta ttggccggta
     2521 ccggaggtgc tctgggtcgg gccgggaacc gatgtcgccg agctaccgcc tctgccggat
     2581 ggctccgcag gtctcgagcc cactgagtcg ggtgggtagc gactcgatag agaacgactc
     2641 gagagctact cttcacgggc ggagcggtgg tacggtcccg cgtgcatggg ctatctgttc
     2701 ggggcgcttg gttacgcgat ggtcgtgctc gtgttgggcg gagctgcaca tgggggggcg
     2761 cggctcggct cgcgctggct cacccataaa cgttttcgct ggttcgacgc cgcgcctccc
     2821 gcttcgtcat tggcccgaag cacgctcgtg cgcacgttca gcgtgttcgc cgcgcttctc
     2881 gtggcgttcg gccttcaggt gatcggcctt ctcgtgagcg ggcacgccga gccaacgctt
     2941 agcgtcgaag tgatccccgg tccagcaaag cgcgccgggc tccagagcgg cgaccgcatc
     3001 gtcagcgtcg acggcaaaaa gctcacgagc ttcgaagaac tgcgcggcga aatccagcgg
     3061 cgcccgggtg agcgacgact cgagctcgat cgcggcgggc agccgctcac tctcttggtg
     3121 acgccggagg acggacgcat cggtgtcatg cctcgttacc gacagctctc gatctcgctg
     3181 cccgctgcac tcgaaggcgc agcaggtgca atctcgaaat acccgcgcac tattctaaag
     3241 agcgcctttt cgccgagcga gcgcacggag cttgcgggcc ccgtcgccat cgtgaatagc
     3301 gttggtgggc agagcaatgg cggctcgcgg gcgctggtcc tcgcgatgat cgcgggagtc
     3361 ttctggccga tcgcgttggg cgtgcacctg ttcgacgcac tcacgctcac gctcttccga
     3421 gcgacgcatg gctgggcggc cagcgccgaa gtggatatcg cgcgtcatgc ccgcatcttc
     3481 cagacgctgc tcgtctcttg cctctgcgcc ctcggttacg tcgtactcgc ggcgattcaa
     3541 gagggagccg attttggaaa cggcgccctg ccgggactca tcgtcttggg tcccgccgcg
     3601 atctcggcgg tgttgctcgt cgggatagcg gcccgcctgc gcttcggcgt cggagtggcc
     3661 attggcttcg tgctcgcgtg cgttacaatc ccgtgtgcgg tcatcgtgct ctccggcgtc
     3721 gcccttcact ggcaacgtct cgagctgcgc cgccgcgggt acgcggtgag ctggttcgtt
     3781 acactacctc ctagcgcggg cccttcgcag ggcgtgctct aaagctcctc gagctccgct
     3841 ccaggagtgc tcgagcattg cacgatcggt cgccccgtga cgatccaccg cctaccccca
     3901 gcaccgtcgt cagcagcgct ccgcgtgggg acgcagaaca gggcggctca tccgcggtcg
     3961 tctaaactcg ccgcgcatct ggcgtcgagc aaggacgcgc cgctcactca gcgtcgtgcg
     4021 agacgccgtg catcgcgccc ctcttcct
//
LOCUS       DASTKS010000958         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1941075, whole genome shotgun sequence.
ACCESSION   DASTKS010000958 DASTKS010000000
VERSION     DASTKS010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941075"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFQ35_20190"
     CDS             <1..339
                     /locus_tag="VFQ35_20190"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9933039.1"
                     /translation="KSLHQAPLLLPLEDSPLRRALSQWFAKLGVVPNVVAEFEDGALL
                     KVFGAEGLGLFPGPAAVIDPIRAQYHVDLVGTTDAVTERFYAISVERRLRNPAVVAIC
                     EAARHELFNS"
     gene            complement(595..1185)
                     /locus_tag="VFQ35_20195"
     CDS             complement(595..1185)
                     /locus_tag="VFQ35_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933040.1"
                     /translation="MLALDLRKRRDGSFQCVTEARYSTLALWLEYDLGLSGGGGMEEF
                     FAELRELREGRRERATTGGDLVYVIVDGDGARLRRTHSKTDCAVSTDELVDAVLRWFR
                     VTNPELADELCALIGVDPPVNVDRKLLEPVIWGPGPSAEFPYRALRGGATWSVRLNDF
                     PAEPMYTLLVDAEEIESFNEWPSHWGRPSEAHTFGE"
     gene            1212..1454
                     /locus_tag="VFQ35_20200"
     CDS             1212..1454
                     /locus_tag="VFQ35_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933041.1"
                     /translation="MHREEKEFSIVLNVAAEFAEDYEGDADGYQWFERFESVLKPRLV
                     AAVFDALRSDPEFRVTHAPRGRDPERVLEIEVLRRV"
     gene            complement(1501..2472)
                     /locus_tag="VFQ35_20205"
     CDS             complement(1501..2472)
                     /locus_tag="VFQ35_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933042.1"
                     /translation="MKAPPSLVTSLALRLSFAPRFALPAAILAALVNTSCVPAMRYEE
                     ANSAAQVEGEAKRRLAAELARTQEKVAALQAELRAHEDGLATREEKLAEETLAKGALA
                     KERDESTTLVEELRAQLTRANERLSAYRSEVERLERELNDARAHAGETAAANGPGAPP
                     TPPPISAAESARLTPAPSSSNGTSGFEEPSRPEPALLREVKTLLARAHLQDRVQARSQ
                     DGKVALRVPADLLFDRDSASLRSGFPLWLEAATTFAAARPALSLRLREIEPDPSLPKN
                     LGRDRREQLTRALGARNLLSRLNWERPKEKDSATPRGYELWLVEKGT"
     gene            2632..3921
                     /locus_tag="VFQ35_20210"
     CDS             2632..3921
                     /locus_tag="VFQ35_20210"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9933043.1"
                     /translation="MSPALVLTRRRWCLGAALSAICWSRVGASRALETPPSLPEVTTP
                     QVTAPREEAVGLPPAPLAERAAPDNSSWTRVDLGHLRVTGDRVLARTQQGEVAELTLD
                     PELTRVAERLLDQAHPVEGAALLIDVQTGRILVWAERRRGGPPGEVITTARAPAASVF
                     KLVTTAALYEKGDVQPQERVCISGGLRSIERRHLDRPDGGPVSCGTFHDALGFSRNAV
                     FAQLATRRLLRDDLVDIAHRLGFNQELPFDWPVRVGELEVPYNDLEFARTAAGFRGST
                     LSPLGAAHLASVIAHNGVAQRFHLVRRTPNFEAPPEGEFVGRVFSAVTAARMARMMEV
                     TVHSGTSRQFFTDELGHSYLPGIRVAGKTGTLRPGSREEMTSWFTGFAPSRKPEVVVT
                     VMLENGKIWRQKAAEVARDVLRAYYARKGAKGVTPPG"
     gene            4107..4427
                     /locus_tag="VFQ35_20215"
     CDS             4107..4427
                     /locus_tag="VFQ35_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933044.1"
                     /translation="MRKAIVEPGSPLEVLLNRARRATKKGELRKAMLTLREACYGVRG
                     DARLWVMYAVACYRVRRRSEATDALRQALWLRERAHDSARAEVIRRLMLRFEDATFGD
                     RSAA"
     gene            complement(4446..>5867)
                     /locus_tag="VFQ35_20220"
     CDS             complement(4446..>5867)
                     /locus_tag="VFQ35_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933045.1"
                     /translation="GVGFIVAAMALLVPYVLNRVERRGFVPFVAARHVRSHKSGFLTI
                     ISVLSIAGVALSSFALCAVVSIMGGFGADLKRKILGNNAHVRVEAQNGGSIGAWNTAL
                     SELRLVKGIRAATPVVSGEAMASSSTNTAGVQLRGVDPVTIGTVIDLVSNVEVGKFKF
                     LEHPEELERLGPDEPIGLGPGGEVYLKGPDLKASARKVDPAVGDAVREESYPGIILGR
                     ELAKSLHVYIGDELTLVSPLGDLGPMGLLPKTQRFRVAAIFFSGMYEYDVSQAYVKLE
                     TAQAFFDLREQVTGIDVRLEDPEHVAEISPLVSKALDDPKLRVRDWKELNKNLFSALK
                     LEKIATFIVLSLAILVASFCIICTLLLMVTEKSKEIAIMKAIGASDQGILRIFMTEGM
                     MIGGIGTVFGVATGLAAALGLRWFGVRLDPDVYYVDRLPINVDAVDYTLVALASFLIT
                     TLATLYPAIAASRLRPVEGIRYE"
BASE COUNT         1005 a         1896 c         1970 g          996 t
ORIGIN      
        1 aagtccttgc accaagcgcc gctactctta ccgctcgaag actcgccgct gcgacgtgcc
       61 ttgagtcaat ggtttgccaa actaggagtc gttccgaacg tcgtcgccga gttcgaggac
      121 ggggccctgc tcaaagtgtt cggcgccgag gggctcggcc tttttccggg gcccgccgcc
      181 gtgatcgatc cgatccgcgc gcagtatcac gtcgacttgg tcggcaccac ggacgcggtg
      241 accgagcgct tctacgccat ttccgtggag cgccgtttgc gcaaccccgc cgtggtcgcg
      301 atttgtgaag cggcgcgaca cgagctgttc aactcctgaa ccagcgcgag cgcgggccac
      361 gaagtaacgt gcgcgactgc cgtctgccgt cgtcgctggc gccgacgcga gcccccgcga
      421 gccctccgcg caccgcgccg gccctcgtta cttacgcggg accgcgacgt cgttataggg
      481 tgcggcttgc gcggtgtagg gtgcactcgc gccgtgtaac gccggtgcaa acgcgccgcc
      541 tcgacgcgcg ggcgcgcccg tttacaccgg gccgcccctc agcgaagcct gtattcattc
      601 gccaaacgtg tgtgcctctg agggccgccc ccaatgcgag ggccattcat tgaagctctc
      661 gatttcttcc gcgtcgacga gcaacgtgta catcggctcg gcgggaaagt cgttgaggcg
      721 cacgctccac gtggccccac cacgcagcgc tcgatagggg aactcggcgc tcggacctgg
      781 gccccatatg acgggttcga gcagtttgcg atcgacgttg acgggcgggt ctacaccgat
      841 cagcgcacac agctcatcgg cgagctccgg attggtgacg cggaaccagc gcagtacggc
      901 atcgacgagc tcatcggtgg aaaccgcgca gtccgtcttg gaatgggtgc ggcgaagtcg
      961 agcgccgtcg ccgtccacga tgacatagac gagatctccg ccggtggtgg cccgctcgcg
     1021 acggccctcc cgcagctcac gcagctctgc gaagaactct tccatgccac ccccaccgct
     1081 cagcccgagg tcgtactcca accagagagc gagcgtcgag tagcgcgctt ccgtcacgca
     1141 ctgaaaggag ccgtcgcggc gcttacggag atcgagcgca agcatgcaat ccacgctaga
     1201 ccagcggcgc catgcatcgc gaagaaaaag agttctcgat cgtactcaac gtagcggcgg
     1261 agttcgccga agactacgag ggggacgctg acggttacca atggttcgag cgcttcgaga
     1321 gcgtgctcaa gccacggctg gtggcggcag tgttcgatgc gctgcgcagc gatccggagt
     1381 ttcgggtgac gcacgccccg cgcgggcgcg atcccgagcg agtgctcgag atcgaagtcc
     1441 tgcgtcgagt ctagtcgtga gcgggagcgc gtccggaaca acgtgaagct agcgcgaagc
     1501 tcaggtgccc ttttcgacga gccagagctc gtaaccgcgc ggagtcgccg agtctttctc
     1561 cttcgggcgc tcccaattca ggcgcgagag caggttgcgc gctcccaggg cgcgcgtgag
     1621 ttgttcgcgt cggtcacggc ctaggttctt gggcaagctg ggatccggct cgatctcgcg
     1681 cagacgaagg ctgagcgcgg ggcgtgcagc ggcgaaggtg gtcgcggctt cgagccacag
     1741 cgggaagcca gaacggagcg aggcgctgtc tcgatcgaac aagagatctg cgggcacgcg
     1801 cagcgcgact ttgccgtcct gactgcgcgc ttgaacgcga tcttggaggt gagcccgtgc
     1861 gagcaaggtc ttgacctcac gcaggagcgc gggctcgggc cggctcggct cctcgaagcc
     1921 gctggttccg ttgctgctgc tgggagcggg ggtcaaacga gcgctctcgg ccgcggatat
     1981 cgggggaggt gtcggaggag ccccgggccc attggcagcg gcggtttcgc cggcgtgggc
     2041 tcgcgcatcg ttcagctctc gctcgagccg ctcgacttca ctgcggtagg cgctgagtcg
     2101 ctcgttcgcg cgagtgagct gggcgcgcag ctcttcgacc agcgtagtgc tctcgtcccg
     2161 ctctttggcg agtgcgccct tggccagggt ctcttcggcg agcttctctt cgcgggtggc
     2221 gaggccgtct tcatgcgcgc ggagttcagc ctggagagcg gcaactttct cttgggtgcg
     2281 cgcgagctcc gcggccagtc gtcgcttggc ctcgccttcc acctgggccg ccgagttggc
     2341 ttcttcgtag cgcatggcgg gcacacagct cgtgttgacg agcgcggcca ggatggcggc
     2401 gggtaacgcg aaccgcggcg caaaggacaa gcgcagggcg agagaggtca cgagtgaagg
     2461 cggcgctttc atgattttcc taaaacggag tagcggcgcg atcgtaagcc ggggcgacga
     2521 cgcggggcca aaatttctcc acgctcgagc cgctaagaaa tcactccgaa aacttggccg
     2581 cccaaatgac gtgaagttag ggtcgcgcaa acgtgggatc ccgcggctga tatgtccccg
     2641 gcactcgtgc tcacccgccg gcgttggtgc ctcggcgcgg cgctctcggc catctgctgg
     2701 tcgagggtgg gagcgagccg tgcgctcgag acgccgccct cgttacccga ggtgaccact
     2761 ccccaggtca cggcaccccg agaagaagcc gtcggcttgc ccccggcgcc gctcgccgag
     2821 cgtgcggcgc cggacaactc gtcttggacg cgcgtcgact tggggcacct gcgtgtgacg
     2881 ggagaccgcg tgctggcgcg cacgcagcaa ggcgaggtgg ccgagctgac gctggatccc
     2941 gagctgacgc gggtcgccga gcgcttactc gatcaagcgc atcccgtaga aggagcagcg
     3001 ctgctgattg acgtgcagac cggtcgaatt ctggtctggg ccgagcgacg acgcggcggg
     3061 ccgcccggcg aggtcattac gacggcgcgc gcaccggcgg cgagcgtgtt caagttggtg
     3121 accacggcgg cgctctacga gaagggggac gtgcagccac aagagcgcgt ttgcatctcg
     3181 ggcggtctgc gcagcatcga gcgtcgacat ctcgatcgcc ccgatggcgg cccggtgagc
     3241 tgcggcacgt tccatgatgc gctgggtttc agccgcaacg cggtgttcgc gcagctcgcg
     3301 acccggcgtt tgctgcgcga cgacctcgtc gatatcgcgc accgactcgg cttcaatcag
     3361 gagctgccgt tcgactggcc cgtgcgcgtg ggagagctgg aggtgcccta caatgacctc
     3421 gagttcgcgc gcaccgcggc cggcttccgg gggagtacgc tatcgccgct cggcgcggcc
     3481 catttggcga gcgtgatcgc ccacaacggg gtggcgcaac gcttccacct cgtgcggcgt
     3541 acccccaact tcgaggcgcc cccagagggc gagttcgtgg gtcgcgtctt tagcgcggtc
     3601 accgctgcac gcatggcgcg catgatggag gtgacggtgc acagcggaac ctcgcgtcag
     3661 ttcttcacgg acgagctcgg ccatagctac ctgcccggga ttcgagtggc gggaaaaacc
     3721 gggaccctgc gccctggcag ccgtgaagag atgacgagtt ggttcacggg tttcgcaccc
     3781 agccgcaagc cggaagtcgt ggtcacggtg atgctggaga acggcaaaat ttggcgacaa
     3841 aaggcggccg aggtcgcgcg cgacgtgctg cgcgcgtact acgcgcgcaa gggcgccaaa
     3901 ggggtcacgc caccgggcta gcgcagccaa gagtgtacag gctcgagggc gcttggttcg
     3961 cgaccgctcg acgcgacttc agctccgctg atcgttttgc gctgcttggc tgtgagctgc
     4021 cgacacgcac ctagcaaact cgcaggttca cgcgtcccga ttcgaaactt cgccgcgggc
     4081 gggccgcttg gtgggctgag cccgtcatgc gcaaagccat cgtcgaacct ggtagtcctc
     4141 tcgaggtgct gttgaaccgt gcccgccgcg ccacgaagaa gggagagcta cgcaaggcca
     4201 tgctgacgct gcgcgaggcc tgctacggcg tccgtggcga tgcgcggctg tgggtgatgt
     4261 atgcagtggc ctgttatcgc gttcgacgtc gcagcgaagc gacggacgcg ctccgccaag
     4321 cgctatggct acgtgaacgc gcgcacgact ccgcgcgcgc cgaggtcata cgccgcctca
     4381 tgctgcgctt cgaagacgcg acgttcggag atcgctcagc ggcctgaacg gcaggcgctg
     4441 gggcactact cgtagcgaat gccttcgacc ggccgcagac gactcgcggc gatcgcgggg
     4501 tacagagtag ccagcgttgt aatcaggaac gaggccagcg ccaccagcgt gtagtcgacc
     4561 gcgtccacgt tgatgggcaa acgatcgacg taatacacgt cagggtcgag ccgcaccccg
     4621 aaccagcgca gtccgagcgc cgcggcgagc ccggtggcca cgccgaacac ggtgccaatc
     4681 ccgccgatca tcatgccctc ggtcatgaag atccgcaaaa tgccctgatc gctcgcgccg
     4741 atcgccttca tgatcgcgat ctctttgctc ttctcggtga ccatcaagag cagcgtgcaa
     4801 atgatgcaga agctcgccac caggatggcg agcgagagca cgatgaaggt cgcaatcttc
     4861 tcgagcttga gcgcgctgaa caggttcttg ttcagttctt tccagtcgcg cacgcgcagc
     4921 ttcgggtcgt ccaacgcctt gctcacgagc gggctaattt ccgcgacgtg ctccggatct
     4981 tccaggcgca cgtcgatacc ggtgacctgc tcgcgcaggt cgaagaaggc ctgcgcggtc
     5041 tcgagcttca cgtaagcttg gctcacgtcg tactcgtaca tgccgctgaa gaagatggcg
     5101 gcgacgcgaa agcgctgcgt cttcggcaag agccccatcg ggccaagatc gccgagcggg
     5161 gaaactaacg tgagttcgtc cccgatgtag acgtgcagag acttggccag ctctcgcccg
     5221 aggatgatgc ccgggtagct ctcttcgcgc acggcgtccc cgacggcggg gtccaccttg
     5281 cgtgccgatg cctttaggtc ggggcccttc aggtaaactt cgccgccggg gccgaggcca
     5341 atcggttcat cgggacccag gcgctcgagc tcctcggggt gctcgaggaa tttgaacttc
     5401 ccgacttcga cgttggagac cagatcgatc accgtgccga tcgtcactgg atccacgccg
     5461 cgcaactgca cccccgccgt attcgtagag ctcgaggcca tagcctcgcc actcaccacg
     5521 ggcgtggccg cgcgaatgcc cttgaccaag cggagctcgg aaagggccgt attccaggcg
     5581 ccgatgctgc cgccgttttg ggcctcgacg cgcacgtgcg cgttgttgcc caaaattttg
     5641 cgcttcagat ccgcgccgaa gccgcccatg atcgacacga ccgcgcacaa cgcgaacgag
     5701 ctcagcgcga ccccggcgat cgagagcacg ctgataatgg tgaggaaccc actcttgtgc
     5761 gagcgcacgt ggcgcgcggc gacgaacggc acgaagccgc ggcgttcgac gcgattcagc
     5821 acgtagggca cgagcaaagc catcgcggcg acgatgaacc ccacgcc
//
LOCUS       DASTKS010000959         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_822679, whole genome shotgun sequence.
ACCESSION   DASTKS010000959 DASTKS010000000
VERSION     DASTKS010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822679"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFQ35_20225"
     CDS             complement(<1..180)
                     /locus_tag="VFQ35_20225"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933046.1"
                     /translation="MHAYPGQLADFVLDHWPDGSRLAMSRRMLAELLSTCFQASLAQE
                     EGRQVRFRMLAAAPSE"
     gene            307..768
                     /locus_tag="VFQ35_20230"
     CDS             307..768
                     /locus_tag="VFQ35_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933047.1"
                     /translation="MVAGGWVALLVSLSLATSGCTLVGAGVGAAVDAAIPGPYDTRAP
                     EQHVRFAPRERIMVWRLNGTRVQGRYLGALGPSARNPETYLVIDAGEQPALIAVSDVR
                     ALGVERSGRGWVYGALIGLVIDVTLVVAAVIAVNNIDFSPRWQSSGSNCWC"
     gene            complement(802..2172)
                     /locus_tag="VFQ35_20235"
     CDS             complement(802..2172)
                     /locus_tag="VFQ35_20235"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET9933048.1"
                     /translation="MTQTHFPKGFVWGAAAASYQIEGAAYADGKGPSVWDMYCEKPGM
                     VWQGQSGEIACDHYHRYAEDVALMKQIGLQAYRLSIGWPRVLPEGVGRVNEAGLDFYD
                     RLIDELLKAGITPWVTLFHWDFPLALYHRGGWLNRDSSDWFAEYTSVIVKRLSDRVTN
                     WMTLNEPQVFIGAGHHEGRHAPGDRLRFAEVLRAGHNCLRAHGRAVQVIRAEARKPAY
                     VGAAPVGLPQLPATNSVEDIAAARQRMFSVWADTAWTNTWWMDPIFLGKYPEDGIARY
                     GSQAPEVRAGDMELIAQPLDFFGANIYQGTPVRAGKEGPEVVAPPVGSPITAFEWAVT
                     PEAAYWGPRFFYERYKKPIVITENGISCRDWVALDGKVHDPDRIDFTARHLLEVGRAI
                     GDGIPVEAYFHWSFIDNFEWAHGYKHRFGLIFCDYPTQRRILKDSAHWYARVIASNGA
                     TLRENP"
     gene            2329..2745
                     /locus_tag="VFQ35_20240"
     CDS             2329..2745
                     /locus_tag="VFQ35_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933049.1"
                     /translation="MSLRQTGLTWVCSLAVSVLVTAACNSGATAPARSPDDEDAPSAA
                     LRNDPAYRADIELMCNVDASIGAERLDPIDTQQKREDFLVEHVKHPDAIYFLTLFRTK
                     PDQERGEMLAERARQLELTKCPLRDRLKGAPLASSR"
     gene            complement(2818..3684)
                     /locus_tag="VFQ35_20245"
     CDS             complement(2818..3684)
                     /locus_tag="VFQ35_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HET9933050.1"
                     /translation="MKQTQPTNQQKSANMTYCVAIKVAAGMVFASDSRTNAGLDQVST
                     FRKMIVYERPGDRFMTMLSAGNLSISQSVREQLQAEKMHVDGGDPLTIWNARSMFDAA
                     RILGQTMRRVDALDGPTLRSNGIDASSTMIFGGQIAGEVPRLFLVYSTGNFIEATPET
                     CYFQIGESKYGKPVLDRILEPTISLADAAKCALISIDSTLKSNLSVGLPIDLVVYRTD
                     ALESDQMVCIDDSNPYFAMVRGSWGQRLREAFTTMQNPSWDSSVGDFPLRVRSERYPV
                     LRKIHNPDEKIV"
BASE COUNT          673 a         1238 c         1244 g          703 t
ORIGIN      
        1 ctccgaaggt gctgcggcga gcatccgaaa ccgaacctga cgcccctctt cttgcgcgag
       61 cgacgcttgg aagcacgtcg agagcagctc ggcgagcatg cgacggctca tcgcgagccg
      121 tgatccgtcg ggccaatggt ccagcacgaa gtcggcgagc tgtccggggt aggcgtgcat
      181 tcgggctaga agcttagacg atccggtgac tgtgcagagg gcggcgcggc tgtgcaccgg
      241 actgcacggg ccacgcgctg gaaccggccg tgcaaggggg cacgcgcgtt gcttcacccc
      301 ggaaacatgg tggctggcgg ttgggttgcg cttctggtct cgctctcgct cgcgacgtcg
      361 ggctgcacgt tggtcggcgc gggggtgggc gccgcggtcg acgccgcaat cccagggccc
      421 tacgatacgc gcgcacccga acaacacgtt cgctttgccc cgcgagagcg catcatggtg
      481 tggcgcctga acggaacgcg cgtacagggg cgctacctgg gcgcgctggg gcccagcgcg
      541 cgcaaccccg agacgtactt ggtcatcgac gcgggcgagc agccggcgct gatagccgtc
      601 tcggatgttc gggccctcgg tgtcgagcgt tcggggaggg ggtgggttta cggagctctg
      661 atcggactcg tcatcgacgt gacgctcgtc gtggccgcgg tgattgcggt gaacaacatc
      721 gatttcagcc cccgctggca gagctctggc tcgaattgct ggtgctgaga gcgttgtctt
      781 ttcgaacctc gggcgcgagc ttcaaggatt ttcgcgcaac gtcgcgccgt tcgacgcgat
      841 gacacgcgcg taccagtgcg ccgagtcttt caggatgcgg cgctgcgtcg ggtagtcgca
      901 gaagatgagc ccgaaccggt gcttgtatcc gtgcgcccac tcgaagttgt cgatgaagga
      961 ccagtgaaag tacgcttcga ccgggatgcc gtcgccgatg gctcgcccga cctcgagcaa
     1021 atggcgcgcg gtgaagtcga tgcgatccgg atcgtgcacc ttgccgtcga gcgcgaccca
     1081 atcgcggcac gagatgccgt tctcggtgat gacgatcggc ttcttgtagc gctcgtagaa
     1141 gaagcgggga ccccaatagg cggcctcggg agtcaccgcc cactcgaacg ccgtgatggg
     1201 gctgccaacc ggcggagcaa ctacctctgg gccctcttta ccggcgcgca ccggcgtccc
     1261 ttggtaaatg ttcgcgccga agaagtcgag cggctgagca atgagctcca tgtcaccagc
     1321 gcgcacttcg ggcgcctggg agccgtagcg cgcgatgccg tcttctggat actttccgag
     1381 gaagatcgga tccatccacc acgtgttggt ccacgccgtg tccgcccaca cgctgaacat
     1441 gcgctgccgc gcggccgcga tgtcttcgac gctgttcgtc gcgggaagct gcggaagacc
     1501 cacgggcgcc gcgccgacgt acgcgggctt gcgcgcttcg gcgcggatca cttgaacggc
     1561 gcggccatgc gcacgcaggc agttgtgccc agcgcgcagc acttccgcga agcgcagccg
     1621 gtctcccggc gcgtggcggc cttcgtggtg gccggcgccg atgaagactt gcggctcgtt
     1681 cagcgtcatc caattggtaa cgcgatccga aagacgcttc acgatgacgc tcgtgtactc
     1741 ggcgaaccaa tcgctcgagt cgcggttcag ccaaccgccg cggtgataga gcgcgagcgg
     1801 gaaatcccag tgaaacaggg tgacccacgg cgtgatcccc gccttcagga gctcgtcgat
     1861 caagcgatcg tagaagtcca ggccggcttc gttcacgcgc ccaacgccct cgggaagcac
     1921 gcgcggccaa ccgatgctga gccggtatgc ctgcagcccg atctgcttca tcagggcgac
     1981 gtcttctgcg tagcgatgat agtggtcgca ggcgatctcg ccgctttggc cctgccaaac
     2041 catgcccggc ttctcgcagt acatgtccca caccgaaggg ccctttccgt ccgcgtaggc
     2101 ggctccctcg atttggtaag aagctgcggc ggctccccaa acgaagccct tcggaaagtg
     2161 agtctgcgtc atggctcggc gcacgctagc acgcgcgtcg ctttgtctgc gcgcgactcg
     2221 cggcgaacgt tatgcaaaaa agctcacccc ttcactttcg tcgggagccg ccgcgcaaaa
     2281 cgactcaagg taagccaagc tcgacgaccc gcaggtgttt caacgctcat gagtctccgt
     2341 caaactggcc ttacgtgggt gtgctcgctc gccgtctccg tgttggtaac cgctgcatgc
     2401 aactcgggag cgacagcgcc ggctcgctcg ccagacgacg aagacgcgcc gtcggcggcg
     2461 ctccgcaacg acccagcgta tcgagctgac atcgaactca tgtgcaatgt cgatgcctcc
     2521 atcggggcgg aacgactcga tcccatcgac acgcagcaaa agcgcgaaga ttttctggtc
     2581 gagcacgtca aacaccccga tgcgatctac ttcctcacgc tgttccgcac caaaccggat
     2641 caggaacgcg gagagatgct cgccgaacgt gctcggcagc tcgagctcac gaagtgccct
     2701 ctgcgcgatc gcctgaaggg cgcgccgctt gcttcctcgc gctgacgagc gcccgggctc
     2761 cgaccccgac gcgaaccgcg cgctcgaccc gacgccgaca cgaaccgcgc accagcgcta
     2821 aacgatcttc tcgtcggggt tgtggatctt gcggaggact gggtagcgct ccgaacgcac
     2881 gcgcagcggg aaatcaccca ccgaagaatc ccagctcggg ttttgcatgg tggtgaacgc
     2941 ctcccgcagc cgctgccccc agctgccgcg caccatcgca aaataggggt tggagtcgtc
     3001 gatgcagacc atctggtcgc tttcgagcgc gtcggtgcgg taaacgacaa gatcgatcgg
     3061 tagacccacc gagagattcg acttcagcgt cgagtcgatg gagatgagcg cgcacttggc
     3121 cgcgtcggcg agcgagatgg tcggctcgag gatgcggtcg agcaccggtt tgccgtactt
     3181 ggactcgccg atttgaaagt agcaagtctc cggcgtcgcc tcgatgaaat tgccggtcga
     3241 gtacacgagg aacagccgcg ggacctcgcc cgcgatttgt ccgccgaaga tcatcgtgct
     3301 gctcgcgtcg atgccgttcg agcgcaacgt ggggccgtcg agcgcatcga cgcggcgcat
     3361 cgtttgcccc aggatgcgtg cggcgtcgaa catgctgcgc gcgttccaga tcgtcagcgg
     3421 gtcgccgccg tcgacgtgca tcttctccgc ttgcagctgt tctcgcaccg attgcgaaat
     3481 cgagaggtta ccagccgaga gcatcgtcat gaagcgatcg ccgggacgct cgtagacgat
     3541 catcttccgg aacgtgctca cctggtctag gcccgcgttc gtccgcgagt cggaggcgaa
     3601 caccattccc gccgctacct tgatcgccac gcaatacgtc atgttcgcgc tcttctgctg
     3661 attcgttggc tgcgtttgtt tcacgagctc tgcgtcgtca cgagttcagg agcgtttcga
     3721 cgctttcctt cacgggcgcg cggccctcga tgaagtcgga gacgcgtgcg tctttcgtgg
     3781 cccgaaactc gtcgacgggc ccgatgctga tgatgcggcc gctgtgtacc atggcgatgc
     3841 gatcgctgat ggtaaaga
//
LOCUS       DASTKS010000960        13317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_153695, whole genome shotgun sequence.
ACCESSION   DASTKS010000960 DASTKS010000000
VERSION     DASTKS010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153695"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFQ35_20250"
     CDS             <1..708
                     /locus_tag="VFQ35_20250"
                     /inference="COORDINATES: protein motif:HMM:NF027969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET9933051.1"
                     /translation="ERVTGFVFKVQANMDPKHRDRVAFVRMCSGHFRRGMKLFHPRSG
                     GTLAVHNPMLFLAQDRDLAEEAVAGDIIGIPNHGKLGIGDALTEGEDLHFIGIPSFAP
                     ELLQRVRPTDPMRAKHLTRALEQLAEEGVARIFKPRIGGYHIVGVVGALQFDVLADRI
                     QSEYEIPVVFEAAGLYTARWVEADDAKLLAEFMSKNEGYLADDHTSAPVFLARNAWHL
                     ETAEKDWPKIRFLKTKG"
     gene            922..2367
                     /locus_tag="VFQ35_20255"
     CDS             922..2367
                     /locus_tag="VFQ35_20255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933052.1"
                     /translation="MRRASRLAAETVEGSEPPAPRAAISQEPNAYLSGEVIADRYRLE
                     RELGRGGMGVVWLAHSLVLGVDVAVKLIRASASGPGVASRMAREAHAAARLGHPALVR
                     VFDFGWTSRSDPFLVMELVLGETLAALLEREARLPAIRAVQMLLPIADGLRLAHDRGI
                     VHRDIKPENIFLASDPLGRAQPKLLDFGIAKVGEKGVDGKLTQVGAVLGSPEYMSPEQ
                     ALGSEEIDGRTDVWSLCIVLYEMVTGTIPFRKPNYNALMQSIINDTPRPASEYGTADA
                     SLWLVIERGLAKSPADRWANMTELGEALALWLYEHGIKEDLSGNSIRAVWLDGLLSGV
                     RVDVAGSVPPIPEGAAPLRERGTAAARANVTTPLEIPAKKRRPSRLALGVAAGALAGA
                     LATALVLRSFGQTEPRPAPSAEARNRAAVALATAVAQPAPSPAPARAQPVEEPRASAS
                     ASTKPRPARPPTARPSAVRRPPEKRSHDFGF"
     gene            2357..3334
                     /locus_tag="VFQ35_20260"
     CDS             2357..3334
                     /locus_tag="VFQ35_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933053.1"
                     /translation="MASERLWRRYAAGFALSFALSVTPHARAQGEVDSETRSIARELA
                     LQGAEAYEQKDYTTALDRFQRAATLFRAPSIVVMLARTLVQTGRFLEALDRYEETQRS
                     PLPPDAPEAFRLAVEDARSEARALRARLPRLEVRTGAGAPRDGLSIKLDGRSVSDALL
                     DVERPIDPGSHQLVVSAAGRVPFHEDFSMTEGERRVITLPALAPLEVDRSSASMAART
                     APSERTRTWALALGGVGLGALGVGVASGIVALGHKSELDSECRPGCPSSARDELSSYR
                     TNRTISYVSLGLGTAALGTGAYLWFLSSKGHASVTARVAPGAISLTGAF"
     gene            3331..4677
                     /locus_tag="VFQ35_20265"
     CDS             3331..4677
                     /locus_tag="VFQ35_20265"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9933054.1"
                     /translation="MRSRRWLAAFSTLALTTLTACPFTDDYYLMNDGGAAGVGGATAG
                     GGTAAAGGGASGACSAANCSGACCSGVCVDLANDRSNCGRCANTCATGRICSVGTCAS
                     GWIPMAAPPAEFAAREKAAYTTFSDKVFVWGGLDANGNELRTGAIYDPALDTWKMSSV
                     EPNTPSGRELATAVWTGSAVLVFGGRAHGDMALKDAALYDPVANRWTKVSDNPTARVA
                     PSAAVYSGMVVFWSGLSGSLMPLGGADRYRVSSDMWNPSPTLNGPSRLGHTACAGVGA
                     SFFVFGGIDSTITGTDKAYRYSFSANTWSTISRGPSARWDAFGATDGASFYVWGGRDD
                     EAVHGDGSRYDTQWVTLEASGAPSPRYAPLRESGWTFALEGPSFALLGGLDSSGAFLH
                     DGGRYLSARDEWGRIAAWPSSEDHAYGVAASVTGELIVFGGRTGPTLSNTGERYGY"
     gene            complement(4684..6234)
                     /locus_tag="VFQ35_20270"
     CDS             complement(4684..6234)
                     /locus_tag="VFQ35_20270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933055.1"
                     /translation="MPPKPIHNTVTLDRIPRVTRVAGQLTVLEGRVAGRKFKIEDSAT
                     IGRSPDASVMLDEPEVSRMHARVSRDGNDSFVIEDLQSKNGVTVNGVRVQRRVLAFGD
                     RIRVGPSIVLEFSGFDAVEEHIVQRQRFEAIGRLGVGIAHDLNNVLAALDAGAAYLRE
                     LPPNRELGDAEVRECIDDLALASARASELTRGILSFARGRGSEHAPVDLSVLVNEVVR
                     MLRHTLDQSIRIQPSVQAHVVVHGSHSELHQVILNLCLNARDAMPDGGLLSISAGMLP
                     EPPDGLGFPAGRGVATLSVADSGTGMDAETQRRIFDPFFTTKREGAGYGVGLATVREI
                     VTLHSGHIALESALGHGSCFTVFLPLFEADRIRLASTEERSPPVTRRPPSSTSILLVD
                     DEQIVRRSVARLLRQAGFDVTEAEDGAEAIARYSRKRFDLVVLDLDMPGLDGEQTQTR
                     LLQLDPSVRVVFASGHVDPKREASVRARGALAFLQKPFALETLMGIAREALNHDFAGD
                     IEEPTRPR"
     gene            complement(6349..7077)
                     /locus_tag="VFQ35_20275"
     CDS             complement(6349..7077)
                     /locus_tag="VFQ35_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933056.1"
                     /translation="MRTNLLGSPLIFVCLALASFGCKRKDAAQCEQAITTVKQALTQE
                     NFTGATQWREYAYKQCDDAAQLSELDQSIVRKRGEVDARTHAAEQRRTENRQVLKVFL
                     QWVADNRAAPDRASATPNCDAAAPPEKVKSKERLCSASRTAGQYPLLLNYWEAEPAIA
                     RFSVKLPDVTSCEEIGASKVLKTWQVPTTSGTTTPRYRCEFPAGPLAGMNAVLSQAVN
                     ADLYVFNPSYIEKDPSVRSMLDGS"
     gene            7426..8700
                     /locus_tag="VFQ35_20280"
     CDS             7426..8700
                     /locus_tag="VFQ35_20280"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9933057.1"
                     /translation="MRNRFSTFEHASFCAGLIMALGIGCSSNTGGTGASGGQASGGRA
                     SGGATSSGGAASGGAAAGATNSGGLANSGGTSSRGGASNSGGASNSGGAASGGAASGG
                     EQSKGGATSGGAASGGEQSKGGATAAGGSSTGGGGMTGGAAGSSTGGQSSGSCPTPAL
                     SSGDTTKKIMVGGLNREYILHVPSTYKGTAPAPLVIDFHPIMGSDTSWRTGSPYPAVL
                     DKEGVVLVYPNGEPSPNLGNAWNVQGCCVSKINGAEVDDVAFAKAIVTEVEKVACIDP
                     KRVYAVGFSMGGGMSNYLACHAADVFAAVGPAAFDLTEQNVPDCKPSRPITVVEWRGK
                     NDNVVSYAGGHSALVTGMAIDFLGAVGTFKKWAELNGCTDSPSAADSNNCQTYSQCMG
                     GVQVTLCTANNGGHEAAKADVVWPMLKKYTLP"
     gene            9138..10130
                     /locus_tag="VFQ35_20285"
     CDS             9138..10130
                     /locus_tag="VFQ35_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933058.1"
                     /translation="MTIELLIHAIVRQTTILIAQLATSRGVRAPLAQIADQVFIELVR
                     ELERQGVSRKVSADMFGLGLRTYRRKIQRLSESSTERGRSLWEVVLEYVKAHKLVTRR
                     EILLRFSHDDEAQIRAVLRDLSESQLIFSSGTGGNSSYRAASDEELITLQKKLGAEGA
                     DELVVAVMYREEPLTLKQIADLAQAEVAAIEAAVHRLRAAGRIEELDIDGETRYRARA
                     LVIPLGAPIGWEAAVFDHFKALVTTILSRLRENRAAALEDQVGGSTYTIDVWPGHPLA
                     DEVYGSLGRIRSMLGDLRSRVVDFNADRELPDAHTRVVIYAGQALIREGGEREE"
     gene            10120..11181
                     /locus_tag="VFQ35_20290"
     CDS             10120..11181
                     /locus_tag="VFQ35_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9933059.1"
                     /translation="MKSEGKHVRSLLLLGAFALAFSCGKQTEDPTGGETHFLTRCDSK
                     AACGSALACLCGVCTLPCDQRAECQGLPGGQCILSSSSGVCTDSAAPGQCDVPCAADV
                     DCRVVSPSHRCEGGACRAGSAPAGTGGSGGSSNTGGSGGLGGETCEHGVTPANAVLLI
                     GDSFFATSHQTTGYLESLARAAGALPAGARYRDNSTLLNNALALNGNGILDQYSRGVA
                     EAPVKVVIMNGGGADLLLGSCNPPDPSCPTLTAAASAAQDLLQRMASDGVQHVLYVGY
                     PDPVDSSVRAKMNAFRPLVQSACENAALPCHFVDLRTTFAGHYGEYIQGDGLNPTDAG
                     SEASASAIWAAMQDRCIAQ"
     gene            complement(11239..12678)
                     /locus_tag="VFQ35_20295"
     CDS             complement(11239..12678)
                     /locus_tag="VFQ35_20295"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9933060.1"
                     /translation="MNRSELNRRRFLAALGGSALAYPFLRGLPSAQAAADPPKYLILL
                     FSPSGFVQHLFGAECAAPTSGTAPTTATPLKFRPSLTSLEPLKDKVTVIEGLNVAAAT
                     TSSHEPGMAALWTGVKTDGSSGNGPSIDQVIAKQLNAGRPFNSIELMVRSSADWTTRE
                     VKTRMIYSAKDMYFDLIDDPVAARQALFPNATSGGGGGGGGTDKKAFIRKKVLANLNS
                     ELTGLKGKLCNQDKVQLDAMQSAWNDLDMQLSQAASAAQSCTAPDPAPSGYKAPSIDF
                     PTSAKLQMDILAMALACDLTRVASLQFSTATSQVTHTWLGSNQNMTHHDLSHQGPSWL
                     GSLGADPDAASGASQYQSLAQLTAIDKWYGDQVYYLANALSKYMVGGKSLLDQTVICW
                     SSEIDLGAAHNHNNMPFVLIGGGGGKLKGNLVARYPVKIEGVQATMGAVDRNHNDLLL
                     TLGQVMGANLSTFGDAALCKGPLSEILAT"
     gene            complement(12675..>13317)
                     /locus_tag="VFQ35_20300"
     CDS             complement(12675..>13317)
                     /locus_tag="VFQ35_20300"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9933061.1"
                     /translation="VPGRKGILTHASFLSAHAHTVLPSPILRGKIVRANIMCRPIQPP
                     STVPSLAGMIPPAPNTPQAGKTTRDLYVAHVSQPVCVGCHQAMDLMGFGLGKFDATGR
                     LYANGMENNVSIDASGEFKNSNSDLDSKTFTDQAQMIDALAASSQVRGCFALEQFRFA
                     LGRVEVEADACSLQQIYSDFSGNNFNLKKLMIAVVKSPAFRNRTVLTAGSDCR"
BASE COUNT         2318 a         4331 c         4363 g         2305 t
ORIGIN      
        1 gagcgggtga ctggattcgt attcaaggtc caggccaaca tggatccgaa gcatcgcgat
       61 cgcgtcgcct tcgtgcggat gtgttcggga cactttcggc gcggaatgaa gctctttcat
      121 ccgcgttccg gcggcaccct cgccgtgcac aaccccatgc tatttctggc tcaagatcgc
      181 gacctcgccg aagaggcggt ggccggtgac atcatcggca tcccgaacca cggcaagctc
      241 ggcatcggcg acgcgctcac cgagggagaa gacctacact tcattggcat cccgagcttc
      301 gcgcccgagc tcttgcagcg ggtgcgtccg acggacccga tgcgggccaa gcacctcact
      361 cgcgccctcg aacaactcgc cgaagaaggc gtggcgcgca tcttcaagcc gcgaatcggc
      421 ggctaccaca tcgtaggtgt cgtcggcgca ttgcagttcg atgtgctcgc ggatcgcatc
      481 cagagcgagt acgaaatccc agtcgtgttc gaagcagcgg gtctttacac cgcacgctgg
      541 gtcgaggcgg acgacgcgaa gctcctcgcc gagttcatga gcaagaacga ggggtatctc
      601 gcagacgacc acacatcggc cccggtcttc ttggctcgaa atgcctggca tctcgaaacc
      661 gccgagaagg attggccaaa gattcgtttt cttaaaacca agggctgagc cccgaatccg
      721 cgcagcgctc gcgcgcgcct cgggccggga gctctcggcg cgagacacgc gcgcatgtgg
      781 ccgcgcgagt cgcgaagcta ccgaaaagtg gttatgcgct cgcctcgagc gccgttctga
      841 atccgataga agccagcgtc gactaggcgc gctgtgtcga cctgagttac ctttacggat
      901 tcgaacatga gcagttagac gatgcgcaga gcgtcccgcc tcgccgcgga gacggtcgag
      961 ggcagcgagc cgcccgcgcc ccgcgcagcg ataagccagg agccgaacgc ctacctctct
     1021 ggcgaggtca tcgcggatcg ctatcgactc gagcgggaac tcggtcgagg cggaatgggt
     1081 gtggtttggc tcgcgcactc gctcgtcctc ggcgtcgacg tcgccgtgaa gttgatccgt
     1141 gcgagcgcgt ccggccctgg tgtggcctcg cgcatggctc gcgaagcgca cgccgccgct
     1201 cggctcgggc accccgcgct ggttcgagta ttcgacttcg ggtggacgag tcggagcgat
     1261 ccatttttgg tgatggagct cgtgctcgga gaaacgctcg cggcgttgct cgaacgcgag
     1321 gcacgcctac ccgcgattcg cgccgtgcag atgctcttgc cgatcgcaga cgggcttcgg
     1381 ctggcgcacg accgcggcat cgttcaccgc gacatcaagc cggaaaacat cttcctggct
     1441 tcggatccgc tcggccgagc gcaacccaag ctgctcgatt tcggcatcgc taaggtgggc
     1501 gaaaagggcg tcgacggaaa gctcactcaa gtgggagcgg tcctcggcag ccccgagtac
     1561 atgtccccgg agcaggctct cggcagcgaa gagatcgatg gacgaaccga cgtctggtcg
     1621 ctctgcatcg tgctctacga gatggtcacc ggcaccatcc cgtttcgcaa gccgaactac
     1681 aacgccttga tgcagtcgat catcaacgac acgccgcgcc cggcgagcga atacggcacg
     1741 gccgacgctt cgttatggct cgtcatcgag cgcggcctcg ctaagtcgcc ggcggaccgc
     1801 tgggccaaca tgaccgagct cggagaggcg ctcgcgcttt ggctctacga gcatggcatc
     1861 aaggaggacc tgtccggcaa ctcgatccgc gccgtctggc tcgacggcct cctctccggc
     1921 gttcgggtcg acgtcgcggg ctccgttccg cccattccag agggcgccgc accgctgcga
     1981 gagcgcggca ccgctgcggc gcgcgccaac gtcacgaccc cgctcgaaat tcccgcgaag
     2041 aagcgccgac cgagccggct cgcgctcgga gtcgcggctg gggcgctggc aggagctctc
     2101 gcgacggcgt tggttctgcg ctcgtttggt cagacagagc cgcgcccggc gccgagcgcc
     2161 gaggctcgca atcgagcagc cgtagcgctc gcgacggcgg ttgctcagcc ggcgccgtcc
     2221 cctgcccccg ctcgcgccca acccgtcgag gagccgcgcg cttctgccag tgcttcgacc
     2281 aagccgcgac cggcgcgccc gccaactgcg aggccaagcg ccgtgcgccg tcctcccgaa
     2341 aagcggagcc acgattttgg cttctgagcg gctttggcga cgctacgcgg caggcttcgc
     2401 gctttcgttc gcactcagcg taacgccgca cgcgcgcgca caaggcgaag tggacagcga
     2461 gacgcggtcc atcgcgagag agctggcgtt gcagggcgcg gaagcttacg agcagaagga
     2521 ctacacgacc gccctcgatc gattccaacg ggcggcgacg ctctttcgcg ctccgtcgat
     2581 cgtcgtgatg ctcgcgcgca ctctcgttca aacggggcgt ttcctcgagg ctctcgatcg
     2641 gtacgaggaa acccagcgct cgccgctgcc ccccgacgcg ccggaggctt ttcgccttgc
     2701 ggttgaggac gcacgcagcg aagcccgggc cttgcgagcg cgcttgcccc ggctcgaggt
     2761 gcgaaccggt gccggcgctc cgagggacgg gctctccatc aaactcgatg ggcgctccgt
     2821 gtcagacgct ctgctcgatg tcgagcgccc gatcgatccg gggtcgcacc agctcgttgt
     2881 ctctgcggca ggacgcgtcc cgtttcacga agatttttcg atgactgagg gggagcgccg
     2941 cgtcatcacc ttgcccgcac tcgcgccgct cgaggtcgac cggagctccg cctcgatggc
     3001 ggcccgaacc gcgccctcgg aacgcacgcg cacctgggct ctcgcgctcg gtggagtggg
     3061 gctcggcgcg ctcggcgtcg gcgtcgcgag cggcatcgtc gcactcgggc acaagtcgga
     3121 gctcgactcg gagtgccggc caggctgtcc ctcgagcgca cgcgacgagc tctcgtctta
     3181 ccgcacgaat cggaccatct cttacgtgag tctcggactc ggcaccgccg cgctcggcac
     3241 gggcgcgtac ctatggttcc tcagttcgaa gggccacgct tcggtgacgg ctcgcgtcgc
     3301 tcctggagcc atcagcctga caggtgcgtt ttgagatcgc gccgctggct cgcggccttc
     3361 tcgacgctcg cgctcacgac gctgaccgct tgcccgttca ccgacgacta ctacctcatg
     3421 aacgacgggg gcgcggccgg tgtcggaggt gcgaccgcgg gcggcggcac cgctgcagcc
     3481 ggcggaggcg caagcggcgc gtgctccgcc gcgaactgta gcggcgcttg ctgctcgggt
     3541 gtgtgcgtcg atctcgccaa cgaccggtcg aactgcggga ggtgcgcgaa cacgtgcgcg
     3601 acggggagga tctgcagcgt gggcacctgc gcgtccgggt ggatccccat ggctgcgccg
     3661 ccagccgagt tcgcggcgcg cgagaaggct gcctacacca cgttttcgga caaggtcttc
     3721 gtgtggggcg gactcgacgc aaacggcaac gagcttcgca ccggagcgat ctacgaccca
     3781 gcgctcgaca cgtggaagat gtcgtccgtc gaacccaaca cgccttcggg gcgtgagctc
     3841 gcaaccgcgg tgtggacggg cagtgccgta ttggtattcg gcgggcgcgc ccatggcgac
     3901 atggctctca aggatgcggc cctttacgat ccggttgcga atcgctggac caaggtgagc
     3961 gacaacccga cggcgcgcgt tgctccgagc gcggccgttt acagcgggat ggtcgtcttc
     4021 tggagcggcc tgagcgggag cctgatgccg ctcggcggcg cagatcgcta ccgagtgagc
     4081 agcgacatgt ggaacccatc gccgaccctc aacggccctt cgcgcctcgg tcacaccgcc
     4141 tgcgccggcg tgggcgcgag ctttttcgtg ttcgggggta tcgactcgac catcaccggc
     4201 acggacaagg catatcggta cagcttcagc gcgaacacgt ggagcaccat ctcccgcggc
     4261 ccgagcgcgc gctgggacgc attcggcgca accgacggcg ccagcttcta cgtttggggc
     4321 gggcgcgacg acgaggcggt ccatggcgac ggcagccgct acgacaccca gtgggtgacg
     4381 ctggaggcga gcggagcgcc gtctccccgc tacgcgccgc tgcgggagag cggctggacc
     4441 tttgcgctcg aaggcccaag cttcgcgctg ctcggtgggc tcgactcgag cggagccttt
     4501 ctgcacgatg gtggccgcta cctgagcgcg cgagacgagt ggggacgcat cgcagcctgg
     4561 ccgtcgtccg aggaccacgc ctacggcgtc gccgcgtccg taacgggtga gctcatcgta
     4621 ttcgggggac gaacgggtcc caccctgagc aacaccggcg agcgatacgg ctactgacgc
     4681 gcatcagcgc gggcgcgtcg gttcctcgat gtcgccggca aagtcgtggt tcagcgcttc
     4741 gcgcgcgatc cccatgagcg tctccagggc gaacggcttc tgcaagaacg cgagcgcgcc
     4801 gcgcgcgcga acagaggcct cgcgcttcgg atcgacgtgg cccgacgcga acacgacgcg
     4861 caccgatgga tcgagctgca ggagcctggt ctgggtctgc tcgccgtcta gtcccggcat
     4921 gtcgagatcc agcaccacga ggtcgaaccg cttgcgtgaa tagcgcgcga ttgcttcggc
     4981 gccgtcctct gcttcggtga cgtcgaagcc ggcttgacgg agaaggcgcg cgacgctgcg
     5041 tcgcacgatc tgctcgtcgt cgaccaggag gatcgaggtc gaggaaggag gccgccgcgt
     5101 cactgggggc gagcgctctt cggtgctggc caggcgaatg cgatctgcct cgaacaaggg
     5161 caaaaacacc gtaaaacagc taccgtgacc gagagcgctc tcgagcgcga tgtgcccact
     5221 gtgcagcgtc acgatttcgc ggaccgtggc aagcccgacg ccgtagccag caccctctcg
     5281 cttggtcgtg aagaacggat cgaagatccg ccgctgcgtt tccgcgtcca tacccgtccc
     5341 gctgtcggcc acgctcagcg tcgcaacgcc gcggccagct gggaacccga gaccgtccgg
     5401 aggctcgggc agcatccccg cggagatcga gagaagccca ccgtccggca ttgcatcgcg
     5461 cgcgttgagg cacaggttca agatcacctg atggagctcg gagtggctgc catgaaccac
     5521 gacgtgcgcc tggacgctcg gttggatacg aatgctctga tcgagggtgt ggcgcagcat
     5581 ccgcacgacc tcgttcacga gcacggacaa atccacgggc gcgtgctcgg agccgcgccc
     5641 ccttgcgaac gagaggattc cgcgggtcag ctcgctggcc cgtgccgaag cgagcgccaa
     5701 gtcgtcgatg cactcgcgca cctctgcatc gccgagctcg cggttcggcg gcaactcgcg
     5761 taggtaggca gctccagcgt ctagcgccgc gagcacgttg ttcagatcgt gtgcgatgcc
     5821 aactccgagg cgaccaatcg cctcgaatct ctgcctttgg acgatgtgct cctcgaccgc
     5881 atcgaagccg ctgaactcga gcacgatgct cggcccaacg cggatcctat ctccgaaggc
     5941 gagcacgcgg cgctgaaccc gaaccccgtt gacggttacg ccgttcttgc tttgtaaatc
     6001 ttcgatgacg aagctgtcgt tcccatcgcg gctcacgcgc gcgtgcatgc gggagacttc
     6061 gggctcgtcg agcatgacgc tcgcgtccgg cgaacggccg atggtggcag agtcctcgat
     6121 cttgaatttg cgtccggcga cgcgtccttc caaaaccgtg agctgacccg cgacgcgcgt
     6181 cacacgaggg atacgatcga gcgtaacggt gttatgaatc ggcttgggtg gcatgaacag
     6241 aggcggccaa ttcgcgcaag gcgacctctg ccgcggcgga agtcgcttaa ctgctgaacg
     6301 gccaccattc agcttggctg aagccgaccc ctgctcaatg ccgtcgcttt acgagccgtc
     6361 cagcatcgag cgaaccgagg ggtctttctc gatgtaggag gggttgaaga cgtacaagtc
     6421 ggcgtttaca gcctggctca agacggcgtt catccccgca agtggcccgg ccgggaattc
     6481 gcagcggtaa cgtggcgtcg tggtgccact ggtggttgga acctgccagg tcttcagcac
     6541 cttggaggcg ccgatttcct cacacgaggt cacgtccggg agcttcaccg agaagcgcgc
     6601 gatggcaggc tcggcctccc agtagttgag caagagcggg tactgaccgg ccgtacgcga
     6661 cgccgaacac agccgctcct tggacttcac cttttccggg ggcgccgccg catcacagtt
     6721 cggagttgcc gacgcccgat ctggggcggc ccggttgtcg gccacccatt gcagaaacac
     6781 cttcagcacc tgccggttct ccgttcggcg ttgctcggca gcgtgcgtcc gcgcgtcgac
     6841 ttcgccccgt ttgcgcacga tcgactggtc gagctccgaa agctgcgccg catcgtcgca
     6901 ttgcttgtag gcgtactcgc gccactgcgt agcaccagtg aaattctcct gcgtgagcgc
     6961 ttgcttcacg gtggtgatcg cttgctcgca ttgagccgcg tccttgcgtt tgcagccgaa
     7021 ggaagcgagc gccaaacaga cgaaaatcaa cggcgatccc aggagattcg tacgcatacc
     7081 ccttcctacc ccgactcttc ggccaccagc aaccttcgcg caaatggggc ttgaatcgca
     7141 gcaaaaaccc gcgcgtcgat gctcgccaaa gcagcttgct ttcacggctc ggccggccgc
     7201 aggcgccccc cctcagccac gcccgcaaag gcggcgcgcc cgcctcgttg caatggccta
     7261 cccgcaccgt gcggtcgtct tccagcgctc gagttcggca ccatgtcgcg cgggttgggc
     7321 gtggatgaaa acttcgaagc tgtgcgctga tgtcggttga ggggggacaa ttttggctct
     7381 ccccgaagag gaacgcgctc tttaaaactg gtcctcggag atttcatgcg gaaccgattc
     7441 agcacgttcg aacacgcgag tttttgtgca ggcctcatca tggcgctcgg catcggctgc
     7501 tcgagcaaca ctggggggac cggcgcgagc ggcggtcagg ccagcggcgg tcgagcgagc
     7561 ggtggcgcaa cgagctctgg aggtgccgcg agcggcggtg cggctgcggg agcgacgaac
     7621 tcgggcggcc ttgcgaacag cgggggcacg agctcgcgcg gcggcgcctc gaacagcggc
     7681 ggcgcctcga acagcggcgg cgcggcgagt ggcggtgcgg cgagcggtgg cgagcaatcc
     7741 aaaggcggcg cgacgtccgg cggtgcggcg agcggtggcg agcaatccaa gggcggagcg
     7801 acggcagctg gagggagctc gactggcgga ggcgggatga cgggcggcgc agccggtagc
     7861 tcgactggcg ggcaatcgag cggcagctgt ccaacgccgg ctctttcatc gggcgacacc
     7921 accaagaaga tcatggtggg tggactgaat cgcgaataca tcctgcacgt gccgagcacc
     7981 tacaagggca ccgcgccggc ccctctcgtc atcgatttcc atccgatcat gggctcggac
     8041 acgagctggc gaacgggctc gccctacccg gccgtgctcg acaaagaagg tgtagttctc
     8101 gtttatccca acggcgaacc gagcccgaac ttgggcaatg cgtggaacgt tcagggatgc
     8161 tgcgtatcca agatcaacgg cgccgaggtc gatgacgtcg cgttcgcgaa agcgatcgtg
     8221 accgaagtcg aaaaggtagc ctgcatcgat ccgaagcgcg tgtacgcggt cggcttttcg
     8281 atgggcggcg ggatgtcgaa ctatctcgcc tgccatgccg cggacgtgtt tgcagcggta
     8341 gggcccgccg cgttcgacct gactgagcaa aacgtgcccg actgcaaacc gtcgcggccc
     8401 attacggtcg tggagtggcg cggcaagaac gacaacgtcg tttcgtatgc tggagggcac
     8461 tcggctctcg tgaccggcat ggcgatcgat ttcctcggcg cggtcggcac cttcaagaaa
     8521 tgggctgagc tcaacggttg caccgatagc cccagcgccg ccgactcgaa caactgtcag
     8581 acgtattcgc agtgcatggg aggtgttcag gtcaccctgt gcaccgccaa caacggcgga
     8641 cacgaagcag ccaaggcgga cgtcgtgtgg ccgatgctca agaagtacac gctgccgtga
     8701 gcgagcgcct caaccggcat cgttccccgc gttcgcgaat cgagtaagag cccgcgcatg
     8761 ggccgtcgcg agcggcgcgg ccccgcatgt ctcaatttgg gcgaaaccgg cgccgcaact
     8821 gacgctcgcc cccgcgcctc tcgaggacgc gaaattgccg gcctgtccgc ggattgacgc
     8881 aaacgccgct cacggcgctc gatacccggc ctatgcatga ccgcatggtc agcgcccgat
     8941 cgctcgacca aggcgacgcg cggttaagcc agaaacctcg gcgggatggg gctcccgacg
     9001 gcggctccca tctgagttgc ctcctcggga tcaggggctc gctccgagct gcgccgcaaa
     9061 aagcacggag gacattgggg tcccctctcg ctcgcgacgc cgcatagtac acttcagggc
     9121 gccgcggcgc gcagccaatg accatcgagc ttttgattca tgccatcgtt cggcaaacta
     9181 cgattctcat tgcgcagctc gcgacgtcac gcggtgtccg cgcgccccta gcgcaaatcg
     9241 cggaccaagt cttcatcgaa ctcgtccgag agctcgagcg acagggagtg agccgaaagg
     9301 tgagcgccga catgttcggg ctcggccttc gcacctatcg aagaaaaatc cagcgtctca
     9361 gtgagagctc caccgagcga ggccgctcgc tctgggaggt cgtgctcgaa tacgtgaagg
     9421 cacacaagct cgtgacgagg cgcgagatct tgctccgctt ctcccacgat gacgaggcgc
     9481 agatcagagc cgttttgcgc gacctgagcg agagccagct gattttttct tcgggtactg
     9541 gagggaactc gtcataccgc gccgcgagcg acgaggagct catcacgctg caaaagaagc
     9601 tcggtgcaga gggtgccgac gagctcgtcg tggcggtgat gtaccgcgag gagcctctca
     9661 cgctgaagca aatcgcagat ctggctcagg ccgaggtggc agcgatcgaa gccgcggtgc
     9721 acaggctgcg ggccgcgggc cgcatcgagg agctcgacat cgacggtgaa actcgctatc
     9781 gagcacgcgc tctcgtgatc ccactcggag cgccgattgg ctgggaagct gccgtgttcg
     9841 atcatttcaa agcgctcgtc accaccatcc tgagccggct cagagagaac cgcgcggccg
     9901 cgctcgagga tcaggtcgga ggcagcactt acacgatcga tgtatggcct gggcatcccc
     9961 tcgccgacga ggtctatggc tcgctagggc gcattcgctc gatgctcggt gacttgcgtt
    10021 cgcgagtcgt cgacttcaac gccgaccgcg agctccccga cgcacacact cgagtcgtga
    10081 tctacgccgg gcaagcgctg atacgcgagg gaggcgaacg tgaagagtga gggcaagcac
    10141 gtgcgctctc ttctcttact cggcgctttc gctctcgcct tcagctgtgg gaagcaaact
    10201 gaagatccca ccggcggaga gacgcacttt ctcacacgct gcgactcgaa agccgcatgc
    10261 gggagcgctc tcgcatgctt gtgcggcgtt tgcacccttc cctgcgacca acgcgccgag
    10321 tgccaaggac tgccgggcgg gcaatgcatc ctttcgagta gtagcggcgt ctgcaccgac
    10381 tcggcggctc cgggtcagtg cgacgtgccc tgcgcggccg acgtcgattg tcgggtggtt
    10441 tcgccgtctc atcgctgtga aggcggggct tgtcgcgccg gctcggctcc agctggaacc
    10501 gggggctccg gaggttcctc gaacacgggg ggcagcggag ggctcggcgg cgaaacctgc
    10561 gagcacggcg tcacccctgc aaacgcggtc ttgctcatcg gcgacagctt cttcgccacg
    10621 tcacaccaaa cgacggggta ccttgagtcg ctcgctcgag ctgccggcgc gctgccggcc
    10681 ggggcgcgct atcgcgacaa ttcgactctg ctcaataacg cgctcgcgct gaacggcaac
    10741 ggcatcttgg accaatattc acgcggagtc gcggaggcgc cggtcaaggt cgtgatcatg
    10801 aatggagggg gcgcggatct gctcttgggt tcttgcaacc cacctgaccc gagttgccca
    10861 acacttaccg ccgcagcgag cgcggctcag gatctcctac agcgcatggc gtccgacggc
    10921 gttcagcacg tcctctacgt cggctatccg gacccggtcg actcgagtgt gcgagcgaag
    10981 atgaacgcgt tccgcccgct cgttcagtcc gcatgtgaaa acgctgctct gccctgccat
    11041 ttcgtcgatt tgcgcaccac gttcgccgga cattacggtg agtacatcca aggcgatggc
    11101 ctcaacccca ccgacgccgg ctccgaggca agcgcgagcg cgatctgggc cgcgatgcag
    11161 gaccgttgca tcgcgcagtg agcgaaccgg cgtctgcgcg ctgctcgccc accgatttcg
    11221 aatccgtacg cggctcgcct aggtcgcgag gatctccgac aacgggccct tgcaaagagc
    11281 ggcgtcaccg aacgtcgaga gattcgcgcc catgacttgt ccgagggtca ggagcagatc
    11341 gttgtggttg cgatcgaccg cacccatcgt cgcctgaacg ccctcgatct tcacggggta
    11401 gcgcgcaacc aggttgccct tcagcttccc gccaccgccg ccgatcaaaa cgaacggcat
    11461 gttgttgtgg ttgtgcgcgg cgccgagatc gatctcgctg ctccagcaaa tcacggtttg
    11521 gtcgagcaag ctcttgccgc ccaccatgta tttgctgagc gcgttggcga gataataaac
    11581 ttggtcgccg taccacttgt cgatcgcagt caattgagcg agcgactggt attgtgaggc
    11641 gcccgaggcc gcgtctggat ctgcgcccaa cgagccgagc cacgacgggc cttgatgcga
    11701 caagtcgtgg tgcgtcatgt tttggttcga gccgagccac gtgtgggtta cctgactcgt
    11761 ggcggtcgag aactgcaagc tcgcgacgcg cgtgagatcg caggcaaggg ccatcgccag
    11821 gatgtccatc tgcaacttcg ccgacgtcgg gaaatcgatc gaaggcgcct tgtagccgga
    11881 cggcgcggga tcgggagcgg tgcacgactg ggccgccgac gcagcttgcg agagctgcat
    11941 gtcgaggtcg ttccaagctg actgcatggc gtcgagctga accttgtcct gattgcacag
    12001 cttgcccttc aagccggtga gctcgctgtt caggttcgcg aggaccttct tgcgaatgaa
    12061 ggccttcttg tcggttccgc cgccgccccc accaccgccc gacgtcgcgt tcggaaacag
    12121 cgcctggcgc gccgcgaccg ggtcgtcgat taaatcaaaa tacatatcct tggcgctgta
    12181 gatcatgcgc gtcttcactt cgcgcgtggt ccagtcggcg ctgctgcgca ccatgagctc
    12241 gatcgagttg aacgggcgcc ccgcgttcaa ctgtttggcg atgacctggt cgatgctcgg
    12301 gccgttgccg gaagaaccat cggtcttcac tccggtccag agcgccgcca tgcccggctc
    12361 gtgcgacgaa gtcgttgccg ccgcgacgtt cagcccctcg atgacggtga ccttgtcttt
    12421 cagcggttcg agcgaggtga gcgacgggcg gaacttcagc ggggtcgccg tcgtcggcgc
    12481 cgtgccggag gtcggcgccg cgcattcggc cccgaacagg tgctgcacga acccgctcgg
    12541 cgaaaaaagg aggatcaagt acttgggcgg atccgccgct gcctgcgcgc tcggaagccc
    12601 gcgaaggaag ggataggcca gggcgctgcc gcccaaagcc gcaagaaacc gcctgcgatt
    12661 cagctcgctt cgattcatcg acagtcactc ccggccgtca ggaccgtgcg attgcggaac
    12721 gcggggctct tcaccaccgc gatcatgagc ttcttcaagt tgaagttgtt gcccgagaag
    12781 tccgagtaga tttgttgcaa cgaacaagcg tccgcctcga cctcgacgcg cccgagcgcg
    12841 aaacggaact gctcgagggc aaaacagccg cgtacctggc tgctcgccgc gagggcatcg
    12901 atcatctgtg cctgatccgt gaacgtcttg ctgtcgagat ccgagttact gttcttgaac
    12961 tcgccgctcg cgtcgatgct gacgttgttt tccatcccgt tcgcgtagag tcgcccggtc
    13021 gcgtcgaact tgccgaggcc gaagcccatc aagtccatgg cttggtgaca gcctacgcac
    13081 accggttggc tcacgtgcgc cacgtacagg tcacgcgtgg ttttgcccgc ttggggggtg
    13141 ttgggcgcgg gcgggatcat ccccgcgaga gagggcaccg tgctcggagg ctggattggt
    13201 cggcacatga tgttcgcgcg cacgatcttg ccgcgcaaaa tcggggacgg gagcacggtg
    13261 tgagcgtggg ccgaaaggaa gctcgcgtga gtgaggatac ctttgcggcc tggcacc
//
LOCUS       DASTKS010000961         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1716698, whole genome shotgun sequence.
ACCESSION   DASTKS010000961 DASTKS010000000
VERSION     DASTKS010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716698"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VFQ35_20305"
     CDS             <1..1445
                     /locus_tag="VFQ35_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933062.1"
                     /translation="FRLFQRPDDAIHPGFDKQTEEDMSGLGLFCSNFQPLTATDAKAI
                     VEEVAVHDAFTAPMGEHVSKNAERADGFSICSARPRLVGGKPSKNPRYLQVRPDVVKV
                     RDRYLAEMGARLYRRIPLPERVIFPVISVLSGRRNNPPDGAIKPLCVYSPIHYQELPE
                     LFMDYVCSVTGKSPSTTGAGTEGALTKGPFNALCATADLNNALVSMLLTGYAGFSSAA
                     GFIGPKFQVDHDISLLVPEVWCRMFPHEREPARLIAEGQIERLEDIDFEGRKVLASRL
                     GYRITAKFVHTFLGRVFDNPADVLTEEILKPETQDLRVFVDGIENIVDAQRRVAEAYF
                     ADGSIDDACPPLRALLHIMAHGHYEGKDAQHPEVRALFSREALLASDWYRERLRTKQE
                     RDVELWRRHVRALTDFLAQPGHHDEAVRLGIPERLAKAKAELERVSSPSYLAGLEGTI
                     GADPVHRGAAVRVMPVRGSREANPAPAFTN"
     gene            complement(1532..2431)
                     /locus_tag="VFQ35_20310"
     CDS             complement(1532..2431)
                     /locus_tag="VFQ35_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933063.1"
                     /translation="MSYERLRMCMKSLSWSLAFVAIGCSAGDPSNPESSRDSVGTSES
                     ELSLRAVCGGPKGLSCKPNQYCAATEIGKCPSKSVYGVCTPRPDVCTDLYSPVCGCDG
                     VTYGNACFAAASGAAVAKKGACEPKPAFCGGIAGIPCPEGEQCVDDPSDDCDPKNGGA
                     DCGGICVSKPSPTFCGGIAAIPCPDGEQCIDDPSDDCDPKNGGADCGGICVPKTNPCA
                     AVLCKVGTECVDKGGVAVCVPVPTDPCAVVRCKAGTQCVNQGGVPVCVPTEPCGNTTC
                     APGLVCCNPLLSICTKPGMVCIQ"
     gene            2830..>3183
                     /locus_tag="VFQ35_20315"
     CDS             2830..>3183
                     /locus_tag="VFQ35_20315"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET9933064.1"
                     /translation="MQPSETAATTAAAEPKSPEASARSAEFPFSLGRATLLSLACGVL
                     YFLSFPGLDLWPLGFVALVPLRIALVGQTPRRAAFLGWVSGMTMVTLGFYWMLNMLQA
                     FSGFATPICVFFVLVV"
BASE COUNT          543 a         1076 c          995 g          569 t
ORIGIN      
        1 agttcaggct ctttcagcgt cccgacgacg cgatccatcc cggcttcgac aagcaaaccg
       61 aagaggacat gtcgggcctc gggctcttct gctcgaattt ccagccgctc acggccaccg
      121 acgcgaaagc catcgtggag gaggtcgccg tgcacgacgc tttcaccgcg cccatgggcg
      181 agcacgtctc gaaaaacgca gagcgcgccg acggcttttc gatctgctct gcgaggccgc
      241 gcttggtggg tggtaagccg agcaagaacc cgcgctatct gcaggtgcgg cccgacgtcg
      301 tgaaagtgcg tgaccgctac ttggccgaga tgggtgcgcg cctctaccgg cgcattccat
      361 tgcccgagcg cgtgatcttt ccagtgatca gcgtgctgtc cgggcgccgc aacaacccgc
      421 cggacggcgc gatcaagccg ctctgcgtgt acagtcccat ccactaccag gagctccccg
      481 agctcttcat ggactacgtg tgttcggtca ccggcaaatc accgagcacg accggagccg
      541 gcacggaggg cgcgctaacg aaggggccgt tcaatgccct gtgcgccacc gcagacttga
      601 acaacgcgct cgtctcgatg ctgctcaccg gctacgcggg gttcagctct gcggcgggct
      661 tcatcgggcc gaagtttcag gtcgaccacg acatcagctt gctcgtgccc gaggtatggt
      721 gccgcatgtt cccgcacgag cgcgagccgg cgcgcctcat cgcggagggg cagatcgagc
      781 gcctcgaaga catcgacttc gaggggcgca aagtgctggc gagccgcctc ggctatcgca
      841 tcacggcgaa gttcgtgcac accttcttgg ggcgggtgtt cgacaacccc gccgatgtcc
      901 tcaccgaaga gattttgaag ccagagaccc aggacctgcg ggtgttcgtc gacggcatcg
      961 agaacatcgt cgacgcgcag cgccgtgtgg cggaagccta cttcgcggac ggcagcatcg
     1021 acgacgcttg cccgccgctc cgcgcgctgc tccacatcat ggctcatggc cactacgaag
     1081 gaaaagacgc gcagcacccc gaggttcgcg ctctcttctc gcgtgaagca ttgctcgcga
     1141 gcgattggta cagagaacgt cttcgcacca agcaagagcg cgacgtcgag ctctggcgac
     1201 gccacgtgcg cgcgctcacc gacttcttgg cgcaacccgg tcaccacgac gaagccgtcc
     1261 ggttggggat ccccgagcgc cttgccaagg cgaaggccga gcttgagcgc gtcagctcac
     1321 cttcgtatct cgcggggctc gaaggaacca tcggcgcgga tccggtgcat cgcggcgcgg
     1381 ccgtgcgcgt gatgccggtg cgaggctcgc gcgaagcgaa ccccgcaccc gcgtttacga
     1441 actgagctcg accgtcgcgg tcacgaaccg agctcgaccg cgttccaaat cgcgcgcgcc
     1501 gctttgaccg cactcaggcg acgcggtgcg ctcattgaat gcacaccatc ccgggcttcg
     1561 tgcaaatcga gagcaacggg ttgcagcaaa cgagccccgg cgcgcacgtc gtgttcccgc
     1621 atggctcggt ggggacacac accgggacgc cgccctgatt cacgcactgc gttccggcct
     1681 tgcagcgcac gacggcgcac ggatccgtcg gcaccggaac gcacaccgca actccgccct
     1741 tgtcgacgca ctcggtgccc accttacaca gcaccgccgc gcacgggttc gtcttcggca
     1801 cacagatgcc tccgcagtcg gcgccaccgt tcttgggatc gcagtcgtcg ctcgggtcgt
     1861 cgatacactg ctcgccatcg gggcagggaa tcgcagcgat tccgccgcag aacgtgggcg
     1921 agggcttgct cacgcagatg cctccgcagt cggcgccgcc gttcttggga tcgcagtcgt
     1981 cgctcgggtc atcgacgcac tgctcgcctt cagggcaggg aatccccgcg attccgccgc
     2041 agaatgcggg cttcggctca caagcaccct tcttcgcgac cgcggctccc gaggcggcgg
     2101 cgaagcacgc gttgccgtaa gttacgccgt cgcagccgca caccggcgag tagagatccg
     2161 tgcacacgtc cgggcgcggc gtgcagacgc cgtacacgct cttgctcgga cactttccga
     2221 tctcggtggc ggcacagtat tggttgggct tgcaggagag tcccttgggc cccccgcaca
     2281 ctgcgcgcaa cgacagctcg ctttcgctcg ttccgaccga atcgcggctc gattcggggt
     2341 tcgacgggtc gcccgcgctg cacccgatgg cgacgaacgc aagcgaccaa gacaacgact
     2401 tcatgcacat ccgaagacgc tcgtatgaca ttgatgaacc tccctgatca tgcagcggcg
     2461 cgtgcacctc tgcgttcgga acatacgcgg cgagagtgac attcggatcg acaaagtgca
     2521 gcatctcatc gaatgaccat ctcgcgtgtc ggcgaacatc gccggttcac gtcgacacgg
     2581 ttcgaaagtg atcgaatcat cccaaaacga tcactcacgc cgttctccca aatcaggtgg
     2641 ttcgcacgct cgagcacttt tccgaagtga ggttgctttg cgcgcgctgc tctccccgtc
     2701 gacgtcggtc ctcacgcgcg gggctacttg ccaccgagcg cgtcctcgtc gcgtcgtctc
     2761 gcgcagccaa atggggcgat ccttcaggtg gctggccggg gccgaagcga gcgaataggc
     2821 ttcgcgccga tgcaaccaag tgagacggcg gcgacgaccg cggcagctga acccaagagc
     2881 cccgaggcga gcgctcgttc cgcggagttt ccgttttctc tcgggcgcgc gacgctgctt
     2941 tcgctcgctt gcggcgtgct ctacttcctt tcgttcccgg ggctcgatct ctggccgttg
     3001 gggttcgtcg cgctcgttcc gctgcgtatc gcgctcgtgg ggcaaacgcc gcgccgcgcc
     3061 gcgttcctgg gttgggtgag cggcatgacg atggtgacgc tcggatttta ttggatgctc
     3121 aacatgctgc aggcgttcag cggcttcgcg acgccgatct gcgtattctt cgtgctggtg
     3181 gtg
//
LOCUS       DASTKS010000962         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1494894, whole genome shotgun sequence.
ACCESSION   DASTKS010000962 DASTKS010000000
VERSION     DASTKS010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494894"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /gene="htpG"
                     /locus_tag="VFQ35_20320"
     CDS             complement(<1..1140)
                     /gene="htpG"
                     /locus_tag="VFQ35_20320"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET9933065.1"
                     /translation="MEPTRHPFQAEVTQVLSLVIHSLYSNKEIFLRELISNASDALDK
                     RRFAAVQDASLLEEGEQLKIRIIPDAAAGTLTIWDNGIGMNEEELKRNLGTIAWSGSR
                     EFVEKLKGASANQELPQLIGQFGVGFYSAYLVADRVTVTSRAAGAEQAFSWESTGQDG
                     FTVAPASRDSVGTSVVLHLKAEQKEYLEPSRLRNLIQHYSDYVAHPIELPKNAANDDK
                     EKSDEFEAVNRASALWQRSPKEVTDEQYNEFYKHLTHDWEEPLAHRHFQIEGAQMFTG
                     LLFVPRRPPFDLFDPEGKHGLRLHVRRVFVMQNCEDLVPRWLRFLRGVVDSEDLPLNV
                     SRETLQDSRIIKAIKKQVVHQSLELLSDIAQNKADEYAKFWEAFGT"
     gene            1580..3112
                     /locus_tag="VFQ35_20325"
     CDS             1580..3112
                     /locus_tag="VFQ35_20325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933066.1"
                     /translation="MPPASTRSPGMVRGRSSPPPLPKTTPPPLPDTAGAPSFSAVRAA
                     AIPRPSSEPPRNPSSSPTKRDIIVPAFGDAQDDAAPPDEDPIGHVDGPISTREPIAKI
                     GRYALFEKFASGGMATVHLGRLDGAGGFTRVVAIKRLLPHLVQDGDFAEMLLKEARLA
                     ARVRHPNVVPTLDVVASKGEVLLVLEYVHGESLSALCRAQAKRNELIPLSIVVSVMME
                     ALEGLHSVHEATDERGRSLGMVHRDVSPPNVIVGVDGMARVLDFGIVKALEQLEETVP
                     NRLKGKTGYMSPEQIRAERLTRASDIFAAGIVLWEMLTLRRFASGAGDKERIDKIMAG
                     NFPNPSDYRPELTEDMDRIVMRALAFEPTERYATAREFAAALGTAAEPASAGVVADWV
                     RSLAEKTLAERARLIAQVENWAANAPEIELNSTPFAVERDVLALASLSGTPAPVGEVQ
                     KRVVKASDAPDSKRAPVKAAAASDAKPAKASAEPQGGRFPMFVLVAALAVIAVVAAYM
                     LR"
     gene            complement(3137..>3764)
                     /locus_tag="VFQ35_20330"
     CDS             complement(3137..>3764)
                     /locus_tag="VFQ35_20330"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9933067.1"
                     /translation="YDASYGGPTTQQIALAYQSNLKLRLGQLNQQGQQKGVDSLLILD
                     LVTLARNRAMSDALLMTGDEDIRVGVQQAQELGVRVHLIGVTPARENQSGTLVQDADT
                     LRELSAEEVRGFLSVTPPVAGGVLASASSGSAYAGPTLSAVARRVANELGPTELETVR
                     HEFSSGLLPSRIDRRLLVAGSSEIGGAALTSEQKRELRAEFIRVLRES"
BASE COUNT          598 a         1239 c         1262 g          665 t
ORIGIN      
        1 ggtgccgaac gcctcccaga acttcgcgta ctcgtcggct ttgttctgcg cgatgtcgct
       61 caagagctcg agcgattggt ggaccacctg ctttttgatc gccttgatga tgcgcgagtc
      121 ttggagggtc tcgcgggaca cgttgagcgg cagatcctcc gagtcgacca cgccgcgcaa
      181 gaaacgcagc cagcgcggca cgaggtcctc gcagttttgc atcacgaaca cgcgccgcac
      241 gtggaggcgg aggccatgct tgccttcggg gtcgaataga tcgaacggcg ggcggcgcgg
      301 cacgaagagc agccccgtga acatctgcgc gccctcgatt tggaaatggc gatgcgcgag
      361 cggctcctcc cagtcgtggg tcaggtgctt gtagaactcg ttgtattgct cgtcggtgac
      421 ttccttgggc gagcgttgcc acagcgcgct cgcccgattc acggcttcga actcgtcgct
      481 cttctctttg tcgtcattgg ccgcgttctt cggcagctcg atcgggtgcg cgacgtaatc
      541 ggagtagtgc tggatgaggt tccggagccg agatggctcg aggtactcct tttgctccgc
      601 tttcaggtgc aacacgacgc tcgtcccgac gctgtcgcgg ctagccggag cgaccgtgaa
      661 gccgtcttgg cccgttgact cccaactgaa agcctgttcg gcgccagcag cgcggctcgt
      721 cacggtgacg cgatctgcca ccaggtaagc gctgtagaac ccgacgccga actgcccgat
      781 gagctgaggg agctcttggt tggcggaggc gcccttcaac ttctccacga actcgcgcga
      841 acccgaccag gcgatggtgc ccaggtttcg cttgagctcc tcctcgttca tgccgatgcc
      901 gttgtcccaa atggtcaggg tgccggcggc cgcgtcgggg atgatccgga tcttcagctg
      961 ctctccctcc tcgagcagcg aggcgtcctg gaccgcggcg aagcggcgct tgtcgagcgc
     1021 gtcggaagcg ttggagatga gctctcgcaa gaagatctcc ttgttgctgt acaaggagtg
     1081 gatgaccaac gagagaacct gagtgacctc ggcctgaaac ggatgacgcg tgggctccat
     1141 gaatttgcgg ccaaagttgc cacgattcga ggggtgtcaa gggtgggctc ggccgtactg
     1201 atcggccgcc gaacatggtc attcggacgc ccctagctcg gccggccgcg cgttcggagt
     1261 gcgagagctc gcgtgtttgc gcgactagca ctaggctccc gcggaacgga tgggattcgt
     1321 tttcgattgc gagtcctgcg ggaagcgcct cacggttccg ccggccctct acgagaagcg
     1381 gtttcgcgga cgtgtagtca cgatctcgtg taaggcgtgc ggcgctccgc ttcgcattga
     1441 tgcgacagtg ccgcctccag cgggaacttc tgagtcaggc ggcgaagcag gcgctccggt
     1501 gccctcgatt ccgcgcgccc cgcgcgttcc aatcgatgct gcgccgcggc catcgaacgg
     1561 caagcccttg cgctcctcga tgccgccggc gtcgacgcgc tcgccgggga tggtgcgggg
     1621 gcgctcgtcg cctccacccc tgccgaagac gacgccgccc ccgctgccgg ataccgccgg
     1681 cgccccttca ttctccgccg tccgcgccgc ggcgatcccg cggccttcgt cggagcctcc
     1741 gcgcaacccg tcttcgagcc cgaccaagcg cgacatcatc gtgcctgcat tcggcgacgc
     1801 tcaggacgac gccgcgccgc ccgacgaaga tcccattggc cacgtcgacg ggcccatctc
     1861 gacgcgtgag cccatcgcga agatcgggcg ctacgcgctg ttcgagaaat tcgcgagcgg
     1921 tggcatggcg acggtgcacc tcggtcgcct cgacggcgcc ggcggcttca cgcgcgtcgt
     1981 cgcgatcaag cgattgctcc cgcacttggt gcaggacggc gatttcgcgg agatgctact
     2041 gaaagaagcg cggctcgcgg cgcgcgtgcg ccacccgaac gtggtcccga cgctcgacgt
     2101 cgtcgcgtcg aagggcgaag ttttgctcgt cctcgagtac gtgcacggcg agtcgttgag
     2161 cgcgctctgt cgcgcgcagg cgaagcgcaa cgagctcatt ccgctcagca tcgtcgtgag
     2221 cgtgatgatg gaagcgctcg agggcttgca ctcggtgcac gaggcgacgg acgagcgcgg
     2281 gcgctcgctc ggcatggtgc accgcgacgt gtcgccgccg aacgtcatcg tgggcgtcga
     2341 cggcatggcg cgcgtgctcg acttcggaat cgtcaaggcg ctcgagcagc tcgaagagac
     2401 ggtgcccaat cgcttgaagg gcaagaccgg ctacatgtcg ccggagcaga tccgcgccga
     2461 gcggctcacg cgcgcttccg acatcttcgc cgcgggcatc gtgctctggg agatgctcac
     2521 gttgcggcgc ttcgcttcgg gcgcgggcga caaagagcgc atcgacaaga tcatggccgg
     2581 caacttcccg aacccgagcg actatcgccc ggagctcacg gaagacatgg accgcatcgt
     2641 catgcgcgcg ctcgcgttcg agccgaccga gcgctacgcc accgcgcgcg agttcgcggc
     2701 tgcgctcggc accgcggccg agccggcctc ggccggagtg gtggcggatt gggtgcgctc
     2761 cctcgccgag aagacgctgg cggagcgggc gcgcctcatc gcccaggtcg aaaattgggc
     2821 cgcgaacgcg cccgagatcg agctgaactc gacgccgttt gccgtggagc gcgacgtgct
     2881 cgcgttggcg agcctttcgg ggaccccggc cccggtcggt gaagtgcaga agcgcgtcgt
     2941 gaaggcttcc gacgcgcccg atagcaagcg ggcgccggta aaggcggccg cggcttccga
     3001 tgccaagcct gcgaaggcga gcgcagagcc gcagggcggt cgcttcccca tgttcgtgct
     3061 cgtcgctgcg ctcgccgtca tcgccgtggt cgcggcgtac atgttgcgct gagccgcgtg
     3121 cggaaccgcg gcgagctcag ctctcgcgca aaactctgat gaattcggcg cggagctcgc
     3181 gcttttgctc gctcgtcagc gcggcgccac cgatctcact cgaaccggcg acgagcagcc
     3241 tccgatcgat ccgtgacggc aagagcccgc tcgagaattc gtgccgcacc gtctcgagct
     3301 cggtgggccc gagctcgttc gcgacgcgac gagcgacggc ggagagcgtt gggcccgcat
     3361 aagccgaacc gctcgaagcc gacgccaaga ctccgcccgc gactggtgga gtcacactca
     3421 gaaaccctcg cacttcttcg gccgagagct cgcgcaaagt gtccgcgtct tggactaacg
     3481 tgccggactg gttctcgcgc gccggagtca ctccaatcaa gtgaacgcgc actccgagct
     3541 cttgagcctg ctgcaccccg acgcggatgt cttcgtcgcc ggtcatgagg agcgcgtcgg
     3601 acatggcgcg attgcgagcg agcgtcacga ggtcgaggat gagcagcgag tcgacgccct
     3661 tttgttgccc ctgctggttc aactgaccga gccggagctt caggttcgac tggtaggcga
     3721 gcgcaatctg ctgcgtcgtc gggccgccgt agctcgcgtc gtac
//
LOCUS       DASTKS010000963         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_599853, whole genome shotgun sequence.
ACCESSION   DASTKS010000963 DASTKS010000000
VERSION     DASTKS010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599853"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /gene="guaA"
                     /locus_tag="VFQ35_20335"
     CDS             complement(<1..1197)
                     /gene="guaA"
                     /locus_tag="VFQ35_20335"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9933068.1"
                     /translation="MGKRQLVLILDYGSQYTQLIARRIRESRVYCEIHPGTLTLDAIR
                     RIEPAAIILSGGPQSVYDAESPRSDPGIFELGLPVLGICYGEQLMAHQLGGKVEPSSE
                     REYGPARVTVKESVGVLGCFAQGEEIAVWMSHGDKLTVPPKGFRAIASSPNAPLTAIA
                     YPEKKLYGIQFHPEVAHTPRGAELLRAFLFEVAGLEPSWTPGSFVDEAIEKVTNTVAP
                     DERVICGLSGGVDSSVAAVLCHRALGDRLVCIFVDNGLLREGEYENVVHTMRQSFHLN
                     LVPVDARKQFLDELKGVTDPEQKRKIIGRVFIEVFEEHAARVENAKFLVQGTLYPDVI
                     ESVSVRGPSATIKTHHNVGGLPERMHLQLVEPLRELFKDEVREVGAAMGLPREMLWRQ
                     PFPGPGL"
     gene            complement(1275..>3348)
                     /locus_tag="VFQ35_20340"
     CDS             complement(1275..>3348)
                     /locus_tag="VFQ35_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933069.1"
                     /translation="ARAHGLGFEIARAADGEADVRRFVTALRAALAEPVRPADPALAR
                     AQREVEALRALTFSGPGEAAVSACSGEIGFERGAPIPDFTSPAGTAELEATRASVFRT
                     GAAAFAALGSTSVLDAATRALGSDAPWPRGDADDDPWPEADGVFVDTGAGPRRLSIAI
                     RSPDTDAVLATGLALRTDRSALSQRLDALSPPWRLERASAVARLRGACLRLDLAPPKG
                     DPAPSPEEIARAALVAEISVQEALAHTEPGAADETVLRPSNPLRAAGVAAWRALLRDD
                     DEEKPRSVLSFASENGDAISAADVGKALAALRARQSRTTIEVSSRPEAGQGELWLLLA
                     SPCGSGLESAADAGAVSLALRSMALGGASEEVELEPWVTSDGAGLLAHAARKDARETA
                     LAEAERVARVLGRAFAQRLGGAEIGTARAALLTQLGGRPFVGWSTLLEGLAPSHPSWL
                     EPRGLWATVSELSGDELERTRRFLLAGPLRLAVLANRGEAQVPAAARTLEDWLMPARG
                     NVSDCSLPRGELPRRGEVRVRQAGAQYEGAYVGVVLAQTGLRGARAAELAELLLNHPR
                     SAMQRGVTARAPGASATARALASVRRPALVVEIRAMPEQLSAAIAVVRAGLDELGRGE
                     FTNEAFAQALARLGEREAEERFDPRRRIVDLFRGASAPELDAGSVKSALSSFTMSSQW
                     TVSTAPPP"
BASE COUNT          524 a         1141 c         1166 g          517 t
ORIGIN      
        1 gagtccgggc cccggaaacg gctgtcgcca gagcatctcg cgcggcagcc ccatggctgc
       61 cccgacctcg cgcacttcgt ccttgaagag ctcgcgcagc ggctcgacga gctgcaggtg
      121 catccgctcg ggcagcccgc cgacgttgtg gtgcgtcttg atcgtggccg acgggccgcg
      181 caccgagacg ctctcgatga cgtcggggta gagcgtgccc tggaccagaa atttcgcgtt
      241 ttcgacgcgg gcggcgtgct cttcgaagac ctcgatgaag acgcggccga tgattttgcg
      301 cttctgttcc gggtcggtga cgcctttgag ttcgtcgagg aactgcttgc gagcgtccac
      361 cggcacgagg ttcaggtgga agctctggcg catcgtgtga acgacgtttt cgtactcgcc
      421 ctcgcgcaag aggccgttgt cgacgaagat gcagaccaag cggtcaccga gcgcgcggtg
      481 acagagcacc gccgcgaccg acgagtccac gccgccgctc aacccgcaaa tcacgcgctc
      541 gtctggcgcg accgtgttcg tgactttctc gatggcctcg tcgacgaagg agccgggagt
      601 ccagctgggt tcgaggcccg cgacttcgaa caggaaggcg cgcaggagct ccgcgccgcg
      661 cggagtgtgc gcgacttcgg ggtggaattg aatgccgtag agcttctttt ccgggtacgc
      721 gatggcggtg agcggcgcgt tggggctcga ggcgatggcg cggaagccct tgggtggaac
      781 cgtgagtttg tcgccgtggc tcatccagac ggcgatctct tcgccttgag cgaagcagcc
      841 gagcacgccg acgctttctt tgacggtaac gcgcgcggga ccatattcgc gttcgctgct
      901 cggctcgact ttgccgccga gctggtgcgc catgagctgc tctccgtaac agatgcccag
      961 caccggcaac ccgagctcga aaatgccggg gtcgctgcga ggggactcgg cgtcgtaaac
     1021 actctggggt ccgccgctca ggatgatggc ggcgggctcg atgcgtcgaa tggcgtcgag
     1081 cgtcaacgtg ccggggtgga tctcgcagta gacacggctc tcgcgaatgc gccgagcgat
     1141 gagctgcgtg tattggctgc cgtagtccag aatgaggacg agctgtcgct tacccatggg
     1201 ttcggcgagg agctaacacg aagcttgctc cgagcaaacc ggcggccacg gcccccattt
     1261 cgctgcggca ggaatcaggg cggcggggcg gtgctaacgg tccactggga gctcatcgtg
     1321 aacgagctca gagcagattt cacgcttccg gcgtcgagtt cgggcgcgct ggcgcctcga
     1381 aataggtcga cgatgcgccg gcgcggatcg aagcgttctt cggcctcgcg ctccccgagc
     1441 cgagccaggg cttgggcgaa cgcctcgttc gtgaactctc cgcgcccgag ttcgtcgagc
     1501 ccggctcgca cgaccgcgat ggcagcgctc agctgctccg gcatggctcg gatctcgacg
     1561 acgagcgcgg ggcggcgaac gctggcgagc gctcgagccg tcgcgctcgc accaggggcc
     1621 cgggccgtga ccccacgctg catggcgctg cgcggatgat tcagcaagag ctccgcgagc
     1681 tctgccgcgc gtgcaccgcg cagcccagtt tgagccagaa cgactccgac gtaggctccc
     1741 tcatactgtg cgccggcttg ccgcacgcgg acctctccgc ggcgtggtag ctcgccgcgc
     1801 ggcaacgagc agtccgagac gttgccgcgc gccggcatga gccaatcctc gagggtacgg
     1861 gcagcagcag gcacctgcgc ttcgccccgg ttcgccagca ccgcgaggcg cagcgggcca
     1921 gctagcaaga agcggcgagt cctttcgagc tcgtcgcccg acagctccga tacggtcgcc
     1981 cagaggccgc gcggctcgag ccaggaaggg tgcgaaggag cgagcccttc gaggagcgta
     2041 ctccacccaa cgaatgggcg gccgccgagt tgcgtgagca gagccgcccg tgccgtgccg
     2101 atttcggccc caccgaggcg ctgagcgaaa gctcgaccga gaacgcgggc gacgcgctcg
     2161 gcctccgcga gcgccgtctc gcgagcgtct ttgcgtgccg cgtgcgcgag gagcccagcc
     2221 ccgtccgaag tgacccacgg ctcgagttcg acctcttcgg aggccccgcc gagagccatc
     2281 gagcgaagcg cgaggctgac ggcgcccgcg tccgccgcac tttcgaggcc ggaaccgcac
     2341 gggctcgcca aaagcaacca gagctcgcct tgccccgcct cgggacgaga gctcacctcg
     2401 atggtcgtcc ggctttgtcg agctcgcaga gccgcgagtg cctttccgac gtcggccgcc
     2461 gaaatggcat ctccattttc ggaagcgaag ctcagcacac tgcgaggctt ttcttcgtca
     2521 tcgtcgcgca gcagagctcg ccacgcggca acgcccgccg cacgcaacgg gttactcggg
     2581 cgaagcaccg tttcgtcggc ggcgcccggc tcggtgtgcg ctagcgcctc ttgcacgctg
     2641 atctcggcga cgagcgcggc ccgcgcgatc tcctctggag acggcgccgg atcacccttc
     2701 ggaggcgcga ggtcgagccg caggcaggcc ccgcgcaaac gagcgaccgc gctcgcgcgc
     2761 tcgaggcgcc acggagggct cagcgcatcc aatcgttggc tcagggcaga ccgatccgta
     2821 cggagagcga gcccggtcgc gagcacggca tcggtgtcgg gcgagcggat cgcgatgctg
     2881 aggcggcggg gcccagctcc ggtatcgacg aagaccccgt ccgcttctgg ccacggatcg
     2941 tcgtcggcgt cacctcgcgg ccacggggcg tcgctgccga gtgcgcgcgt ggccgcgtcg
     3001 aggacgctcg tgctgccgag cgcggcaaat gcggcggcgc cggtgcgaaa aacggacgcg
     3061 cgcgtcgctt cgagctccgc cgtccccgcc ggactagtga agtcggggat gggtgcgccg
     3121 cgttcgaagc cgatttcgcc cgagcacgcc gaaaccgccg cctcgcccgg acccgaaaag
     3181 gtgagggcac gcagcgcttc gacttcgcgt tgcgctcgcg ccagcgccgg atctgctggg
     3241 cgcactggct ccgcgagcgc cgcgcgcagc gcggtcacga accgccgcac gtccgcttcg
     3301 ccgtcggccg cacgcgcgat ctcgaagcca aggccgtgcg cacgcgct
//
LOCUS       DASTKS010000964         9219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1495236, whole genome shotgun sequence.
ACCESSION   DASTKS010000964 DASTKS010000000
VERSION     DASTKS010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495236"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..732
                     /locus_tag="VFQ35_20345"
     CDS             133..732
                     /locus_tag="VFQ35_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933070.1"
                     /translation="MTDTSALLVGLGLGIRHATDADHLAVVTALARHEQSRLQAIRIA
                     ALWSLGHVGSFLSIGLLVVILDMQLPRAVERALDLLVAAMVFTLGAIQLTARSGNQGS
                     VRARPVIIGLVHGLAGSAGIALLVATTTGSRLAAALYLALFSGGTVLGMAAMTAVISF
                     PIRWSHQQPDHVRRWLFRLPGALSVAVGIGLLVDVLSTP"
     gene            822..3419
                     /locus_tag="VFQ35_20350"
     CDS             822..3419
                     /locus_tag="VFQ35_20350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9933071.1"
                     /translation="MPERLKYKCACCLCAACLGAFLYPRSSLAAGIEPPRIREQTAPR
                     LPGHLSSARNASVELSITVTADGAVRDPEVVGSPGEEYSQAALDAVKTWVFEPARRGG
                     RSVAARVRVLVPFLAPAPSTTEDPKANASGVHSEQARAPAVSSSDFTRSDNTTAAEAS
                     PSNGGERASDAAPAVEVLVRGRAEPPSRGASDYRVRVGELSRVPRKNSSELLKLAPGV
                     FLTNEGGEGHAERIYLRGFDAREGQDIEFSVGGVPVNESGNLHGNGFADLHFVIPELV
                     HSLRVVEGPFDPRQGNYAVAGSADYELGLDQRGLTLKYAQGTFGTKRALVTFGPPGES
                     AHTFGGAELYETRGFGRNRDARRATAMGQYEGSLGDNGTFRLSGAAYANTYHAAGLVR
                     EDDFVAGRLGFFDTYDPRQGGSGSRFQLAFDVETKSAGILFHQQVFAVARSMRLRENF
                     TGFLLDTQEALQSPHAQRGDLLDLDMSEGMLGARGFARARSKAFGRTHDLELGYFARG
                     DVAEASRQRIEAASGAPYKTDVALRSKLADIGLYADVAFHPWAWLSLRGGFRADFFAF
                     DVLDACAVQDVSLPSPSKPAVDESCFDQQRGGEHREPNQRSSTGSSKIMPRVTVIAGP
                     FNHVTFSGSLGTGVRSIDPSYITDDVATPFASIEAVEGGVSYSRSFDALSVAARSVFF
                     TTHVDRDLVFSETEGRAVLGAGTRRTGWAGTLRLTNAFLDESANVTLLRSSFDDTGLL
                     VPYTPDLVFRSDTALGSELPFSILQSALRGSLGVGMSYVGRRALPYGQRSETMFLVDG
                     AASLRFRGAELSVTVMNLFDSRYRLAEYEYVSAFRSNASPTLVPTRHFAAGAPRGVYF
                     SLSLNLGGG"
     gene            3416..4189
                     /locus_tag="VFQ35_20355"
     CDS             3416..4189
                     /locus_tag="VFQ35_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933072.1"
                     /translation="MMRKVLGCVLFVCAPGCVGTTGGDAFDFEAFASGPRAADGRAYS
                     FANGRGYSVTLTRARVHVGGVYLNQSVQTQVASNPSCTLSGIYVAEVTSPLEIDVLSP
                     QLQAFSGSGRAISEPAFAGEVWLNSGDVNAVHDSTPVLDAAGSAVKDGTSYPFAASLT
                     IGSNRAKSPSPSTPGAAPICKQRIVSPIPVELTPRRGGHLVIEIDPARMFANVDFADL
                     TPDRDGVFVFEDDDTPGPSLSLYNGLRRSSGVYELRWEP"
     gene            4250..5455
                     /locus_tag="VFQ35_20360"
     CDS             4250..5455
                     /locus_tag="VFQ35_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933073.1"
                     /translation="MSSHSRTIISALLPTVALALCSACGDNAKTSDSGGAGGAGGQTH
                     DAGRTINVQIGGEELATDGILFPEGSEVTLVDGWEITFEHVLVTVANVTLSENPDRSP
                     SDQSQTGAVVAQVTGPWAVDLHKNGSVAAAGGEGTAIPLFTIANQNKKGNAPFDMTQR
                     YAFSYDIVSASDAATKVNFEDDVEAKAAYQEMVRNGYSVLYVGTATFKGQGCQSTDDT
                     YDFGKIPVTIPFEIGFATPARMINCQNQENEGDAFQGEEFQRGIPIYANRSSVAQVTL
                     HVDHPFYSDVQHEPALHFDQFAAQLVAKPETQRLLTIEDVVGVDPTAFTDAQGAMLPW
                     RACDGSALPATTQMAFDVGSVPFDPSAEPSEALRDYRDYAYYVQSTQGHLNGGEGLCF
                     VQRNYPYPR"
     gene            complement(5609..7372)
                     /locus_tag="VFQ35_20365"
     CDS             complement(5609..7372)
                     /locus_tag="VFQ35_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933074.1"
                     /translation="MRTLRDGSLGFLGCVSAIVGCSPQVFIAQGEGGSSGQFGSGGGT
                     SASGGAAASGGGSATFGGANSASGGRIGAGGSGGGLATTGGAGGSDVGGALSTGGALS
                     TGGAFSTGGALSTGGTIGVCKLGADQSCNDELSVSSIWGHCQADGTCLCNPGREINPA
                     TGKCRLSAAARCYSPNQNLDTAYMDGSVGCACDPAKPSSVCRRSPSGTDVALECGTDG
                     HWHAVQDGACASNPLGCRIRRSVVLTSEVALRSDVHFSRNAGQFSYTAIDNPESGSSR
                     VSWGKATWLGVQQEGAFATNLELIMGAGLLASDTTGERVVGVSGAATTNSILAQGFSW
                     QNDVFQSGGALFGTFDVPSSRTYDVKTSLDGQRLVFAFGEMFAETSARFALLDVEAQA
                     VGVAREFDLNTTQWTCLSALPTQSGGALSALANDPNRGWLAKIFELDGSGAPAFSAEY
                     PLDGLPAQPSVCPLVARTDDRYFVGYRDTSGAPRLLTFVRNTTDQASWPLDIGPDSRM
                     DGLAVVNGVAVVIGRGVAGDLSAHLSGPPLFSSTGVSPLGEVATSTVQLISTEGGSIY
                     VSYLTPSERVLAEVACVWPIL"
     gene            complement(7419..8213)
                     /locus_tag="VFQ35_20370"
     CDS             complement(7419..8213)
                     /locus_tag="VFQ35_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933075.1"
                     /translation="MSDTRPWLESKAAPESVLALLRHAKPPGALDAVTRERSRRRLAQ
                     ISALPAAAGAFLWLKHVALGAVLGSAALAVVSLPELLHQRTSAPSPSAAAAPARQRRV
                     APLATGASQSAAASNASESAAPSALQSASPASAWSGDIGAPAPRKTVAEAKASVGAAS
                     AKSEPESELVREARLLDLARGRLSTDPAGALVMLAQHERDFGGGALGLEREFLSVAAL
                     VKLGRRAEAEARAERLRTLAPGSLYQERLTAVLHPTAGAEPTRSKP"
     gene            complement(8210..8779)
                     /locus_tag="VFQ35_20375"
     CDS             complement(8210..8779)
                     /locus_tag="VFQ35_20375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933076.1"
                     /translation="MTSASPLSNVASPAMPADVAAVHEAHADFVWRTLQRLGVHQQDL
                     EDVLQEVFLVVHKRLSTFDHSSQLTTWLFGICLRVAAAYRRRAHRRHETLGLGPEEAL
                     LRDGAADPEQVLAQREALRRLELALETLGLERRAVFVMFEIEGISAPAIADIMGVPVG
                     TIYSRLSTARAEFTRAVERMEKQEKRGRP"
     gene            complement(8935..>9219)
                     /locus_tag="VFQ35_20380"
     CDS             complement(8935..>9219)
                     /locus_tag="VFQ35_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933077.1"
                     /translation="RWALSACATLGVGAMHAVVRELTPLDPGDRVFAAASLGPELSVG
                     LAAGIRAELGAMAFVPLVRPEFVQRGVAEPVFESRVVGVLGLAGLAFQTR"
BASE COUNT         1624 a         3084 c         2970 g         1541 t
ORIGIN      
        1 ctacgacgag taggctgcca acgacgagta ggctgccaac gacgagtagg ctgccaacga
       61 cgaccagacc gcgagcgtgt cgttttcgca actcagttgc attactgcac agctcgggct
      121 agaagggccg cgatgactga cacgtcagcg ttgctcgtcg ggcttggact tggcattcgt
      181 cacgccaccg acgccgacca cttggcggtc gtgacggcgt tggctcgaca cgagcagagc
      241 cgacttcagg ccatccgcat cgccgcgttg tggtcgctag ggcacgtggg ctcattcctg
      301 agcattgggc tgctggtcgt catcctcgat atgcagttgc cgcgcgcagt agagcgggcg
      361 ctcgacctct tggtcgcggc catggtgttt accctcggtg ccatccagct cacggcgagg
      421 tccgggaacc aaggttccgt gcgcgctcgc cccgtcatca tcgggctggt gcacgggctg
      481 gccggatccg cggggatcgc gttgctggtt gcgacgacga ccggctcgcg gctagcggcc
      541 gcgctgtacc tcgcgctttt cagtggcggg acagtgctcg gcatggcggc catgaccgcc
      601 gtcatctcat ttcccattcg ctggtcgcac cagcagccag accatgttcg gcgctggctg
      661 tttcggctcc cgggagcgtt gagcgtcgcg gtcggcatcg gcttgctcgt ggacgtgctc
      721 tcgacgccat gaaccggcga acgcgacctc gctcattgca actccggcgc agaaactggc
      781 tggcgccgta gaggctggcc accgctccgc cttcgattca catgcctgaa cgactcaagt
      841 acaagtgcgc gtgctgccta tgcgcggcct gtctcggcgc gttcctctac ccgaggtcat
      901 cgctcgccgc ggggatagag ccgcccagga ttcgggagca aacggctccg cgattgccag
      961 gccacctctc tagcgctcga aatgcttcgg tcgagctctc gatcacggtc accgctgacg
     1021 gcgctgtgcg cgacccagaa gtcgtgggca gcccgggcga agagtactcg caggcggcgc
     1081 tggacgcggt caaaacgtgg gtcttcgagc ccgcacgacg cggtggccgt tcggtggcag
     1141 cgagagtgcg cgtcctcgtg cccttccttg cgcccgcgcc gtcgaccacc gaagatccca
     1201 aggcaaacgc gagcggagtc cattccgagc aggcacgggc gcccgcggtc tcctccagcg
     1261 atttcacgcg ctctgacaac accacggcgg ccgaggcctc gccttccaac ggaggcgaac
     1321 gcgcgagcga tgcagcacct gccgtcgagg tcttggtccg cgggcgcgcc gagccaccat
     1381 cgcgcggcgc gtcggattat cgcgtgcgcg tcggggagct gtcgcgagtt ccgcgtaaga
     1441 actcgagcga gctactgaag ctcgcgcctg gagtattcct taccaacgaa ggcggcgagg
     1501 ggcacgccga gcgcatctac ctacgggggt tcgacgcacg ggaagggcag gacatcgagt
     1561 tcagtgtcgg cggggtcccc gtcaacgaaa gcggcaactt gcacggcaac ggcttcgccg
     1621 atctgcactt cgtgatcccc gagctcgtgc actcgctgcg agtcgtcgag ggaccgttcg
     1681 atccgcgcca gggcaactac gccgtcgcgg ggagcgctga ttacgagctc ggcctcgatc
     1741 agcgaggcct cacgctcaag tacgcgcaag ggacgttcgg caccaagcgc gcgctcgtta
     1801 ccttcggccc tccgggcgaa agcgcgcaca cgttcggcgg agccgagctc tacgaaacgc
     1861 gaggcttcgg ccgcaaccga gacgcgcgcc gagcgaccgc gatgggtcag tacgaaggct
     1921 cgctcggcga caacggtacg ttccggttga gcggggccgc ctacgcgaat acgtaccacg
     1981 ccgcaggtct cgttcgcgag gacgacttcg tggccggaag gctcggattt ttcgacacgt
     2041 acgatccgcg gcagggcgga agtgggtccc gttttcagct ggccttcgat gtcgaaacca
     2101 aatctgccgg catccttttt catcaacagg tcttcgccgt cgctcgcagc atgcggctgc
     2161 gcgagaactt caccggcttc ttgctcgaca cgcaagaggc cctgcagtcg ccacacgcgc
     2221 agcgcggcga tcttctcgat ctcgacatga gcgagggcat gctgggagcc cgcggcttcg
     2281 cgcgcgcgcg ttcgaaggcg ttcggcagaa cgcacgacct cgagttaggc tatttcgcgc
     2341 gcggtgacgt ggctgaggcc tcgcgccagc gcatcgaagc cgcgagcggg gcgccctaca
     2401 agaccgacgt ggcgttgcgc tccaagctcg ccgacatcgg tctctacgcc gacgtggcgt
     2461 tccatccatg ggcgtggctc tcgctgcgcg gcggctttcg cgccgacttc ttcgcattcg
     2521 acgtcctcga cgcctgcgcc gtgcaggacg tatccctgcc ctcgcccagc aagccggcgg
     2581 tcgacgagag ctgcttcgat cagcagcggg gcggcgagca tcgggagccg aaccaacgct
     2641 cctcgacggg gagctcgaag atcatgccgc gcgtgacggt gatcgcgggg ccgttcaacc
     2701 acgtcacctt ctcgggaagc ctcgggaccg gcgtacgctc catcgatccg agctacatca
     2761 ccgacgacgt cgccactcca ttcgcgagca tcgaggcggt cgaaggcggt gtcagctact
     2821 cgcggagctt cgacgcactg agcgtggccg cgcgctccgt gttcttcacg acgcacgtcg
     2881 accgagacct cgtcttcagc gagacagagg gtcgcgcggt gctcggcgct ggcacgcgac
     2941 gcaccggctg ggccggcacg cttcgcctca cgaacgcttt cctggacgag agcgcgaacg
     3001 tgacactgct caggtcttcg ttcgacgaca cgggcctgct ggtgccctac actccggatc
     3061 tggtgttccg ttcggacacc gcgcttggca gcgagctgcc cttctccatt ctacagagcg
     3121 cgctgcgggg ctcgctcggc gtcggcatga gttacgtggg tcgacgggcg ctcccttacg
     3181 gtcagcgaag cgagaccatg ttcctcgtcg acggcgcggc gtcacttcga tttcgcgggg
     3241 ccgaattgag cgtcacggtc atgaacctgt tcgactcgcg ctaccggctc gcggagtacg
     3301 aatacgtctc ggcgttccgc agcaacgcat cccccaccct ggtgccgact cggcacttcg
     3361 cggcgggtgc cccgcgcggc gtctatttct ctctttcgct caatttggga ggtggatgat
     3421 gcgaaaggtc ttgggttgtg tgctcttcgt gtgcgcgccg ggttgcgtcg gcacgacggg
     3481 cggggatgcc ttcgatttcg aagctttcgc gagtggaccg cgcgctgcgg acggtcgggc
     3541 ctactcgttc gcgaacggcc gcggatactc ggtgacgctg acacgcgcac gagtccacgt
     3601 cggcggcgtc tacctgaacc aatcggtgca aacccaggtc gcgtcgaacc cgagctgcac
     3661 gctttccggc atctacgtcg cggaggtcac ctcaccgctc gagatcgacg tcctgtcacc
     3721 gcagctgcag gccttttctg gctctggccg cgccatttca gaaccggcgt tcgcgggtga
     3781 ggtttggctg aacagtggcg acgtgaacgc ggtacacgat tcgacgccgg tcctcgacgc
     3841 cgcgggtagc gcggtgaagg acgggaccag ttatcccttc gcggccagcc tgaccatcgg
     3901 ctcgaaccgc gcgaagagcc cgtctccgag cacacccgga gcggcgccga tttgcaagca
     3961 gcgcatcgtc tccccgatcc cagtggagct aactccgcgt cggggagggc atctcgtcat
     4021 cgagatcgat cctgcgcgga tgttcgccaa cgtcgacttc gccgacctga ctccggatcg
     4081 cgacggggtg ttcgtgttcg aggacgacga tacccccggg ccgagcctca gcctctacaa
     4141 cggtctacgc cgcagctcgg gtgtctacga actgcgctgg gagccgtagc gagcgcgcgc
     4201 tttgcgccgt ttccgaccat ttccaactca cgactaggag aacctcgaaa tgagcagcca
     4261 ttcgcgaacg atcatcagcg ccctcttgcc gaccgtggcc ctcgcgcttt gtagcgcctg
     4321 cggagacaat gccaaaactt cggattcggg aggggccggg ggcgcgggcg ggcaaacgca
     4381 cgacgcgggc aggaccatca acgtgcaaat cggcggcgaa gagctcgcga ccgatggaat
     4441 tcttttcccc gaggggagtg aggtcacgct cgtagacgga tgggaaatca ctttcgagca
     4501 cgtactcgtc acggtcgcga acgtcacgct ttcggagaac cccgacagat cgccttcgga
     4561 tcagtcacag acgggcgccg tcgtcgcgca ggtgacgggc ccgtgggcag tcgatttgca
     4621 caagaacggg agcgtcgccg ccgccggcgg tgagggcacg gcgatccctc tttttaccat
     4681 cgcgaaccaa aacaagaagg gcaacgcgcc tttcgacatg acgcaacgct acgctttcag
     4741 ctacgacatc gtgtccgcta gcgacgccgc taccaaggtc aacttcgagg acgatgttga
     4801 agccaaggcg gcataccaag agatggtacg gaatggctac tccgtgctct acgtcggcac
     4861 ggcgactttc aagggccaag gctgtcaaag cacggacgac acctacgact tcggcaaaat
     4921 cccggtgacg attcccttcg agatcggctt cgcgacccct gcgcgcatga tcaactgcca
     4981 aaatcaggag aacgagggtg acgctttcca aggcgaagag ttccagcgcg ggatccccat
     5041 ctacgcgaat cgaagttcgg tcgcccaggt gactctgcac gtcgaccacc ccttctactc
     5101 ggacgtccag cacgagccgg cgctgcactt cgatcaattc gcggcgcagc tcgtcgcaaa
     5161 gcccgaaacc cagcgcctac tcacgatcga agacgtggtt ggtgtcgacc cgaccgcttt
     5221 caccgacgcc cagggcgcga tgttgccgtg gcgcgcttgt gacggctcgg ccttgccggc
     5281 gactacgcaa atggcgttcg acgtcggctc cgtgccgttc gatccctcgg cggaaccctc
     5341 cgaggcgctt cgcgactatc gcgactacgc ctactacgta caaagcacgc aggggcacct
     5401 gaatggcggt gagggtctct gcttcgtgca gcgcaactac ccatatccca ggtaggtgat
     5461 gcgtgagtga ggcaggcgac caagacgctg aaagggccgt cgagcccgat gggcgcgggt
     5521 cgcaagcttc gccggtggaa acggcgctgc aaagagtccc cgtgtgccgc cacaaatcaa
     5581 ggctagtcaa acgggtcggt gttctagcct atagaatcgg ccaaacgcac gccacttcgg
     5641 caagcacccg ctcggaggga gtcaggtagc tcacgtatat cgagccgcct tccgtcgaaa
     5701 tcagctgcac cgtcgatgtc gccacttcac ccaacgggga gacgccggtg ctgctgaaga
     5761 gtggcggccc actgaggtga gcgctcaggt cgcccgcaac tccacgccca atgaccacgg
     5821 ccaccccgtt gacgacggcg agcccgtcca ttcgcgagtc cggtccgata tcgagaggcc
     5881 acgaagcctg atcggtagtg ttgcgcacga aggtgaggag gcgcggtgca ccgctggtat
     5941 cgcggtagcc gacgaagtac cggtcgtcgg tgcgcgcgac gagcgggcaa acactcggct
     6001 gagcggggag gccgtcgagc gggtattccg ccgagaaagc cggagcgccg ctgccgtcga
     6061 gctcgaagat tttcgcaagc caaccgcgat tgggatcgtt cgccagtgcg ctcagcgcgc
     6121 cgccgctctg ggtgggcagg gcgctcaaac atgtccattg ggttgtgttc aggtcgaact
     6181 ccctcgcgac accgaccgct tgcgcctcaa cgtcgagcag cgcgaagcgc gccgacgtct
     6241 ccgcgaacat ctcgccgaac gcaaagacga ggcgctgtcc gtcgagtgac gtctttacgt
     6301 cataggtgcg actggagggg acatcgaaag ttccgaacag cgctccgccc gattgaaaga
     6361 cgtcgttctg ccaagagaag ccctgagcca agatcgagtt cgtcgtcgcg gcgccgctga
     6421 ccccgaccac gcgctcgccg gtcgtgtcac tagcgagcaa gcccgcaccc atgatgagct
     6481 cgagattcgt ggcgaacgca ccttcttgct gcacgccgag ccaagttgct ttgccccaag
     6541 aaactcgcga actccccgac tcgggattgt cgatcgcggt gtaagagaac tgcccggcgt
     6601 tgcggctgaa gtgcacatcg ctgcgcaacg cgacttcact ggtgagcacg acgctgcgcc
     6661 gaatgcggca gccgagcgga ttcgaagcac aagcgccatc ctggaccgcg tgccagtgac
     6721 catccgttcc gcattccagc gctacgtcag tgccgctggg gctgcggcga cacacgctcg
     6781 aaggctttgc tggatcacaa gcacagccca cggagccatc catgtatgcc gtgtcgaggt
     6841 tttggttcgg cgagtagcag cgcgcagccg cactcaggcg acacttgccc gtcgcggggt
     6901 tgatttcgcg accggggttg caaagacaag tgccgtccgc ctggcagtga ccccaaatac
     6961 tcgaaacgga gagctcgtcg ttgcagcttt gatctgctcc gagcttgcat actccgatgg
     7021 tgccgccggt gctgagcgcg ccaccggtgc tgaacgcgcc gccggtgctg agcgcaccac
     7081 ccgtgctgag cgcgccgcca acgtcactgc cgcccgcgcc gccggtcgtg gcaagaccgc
     7141 cgccggatcc acccgcaccg atgcgcccgc ccgacgccga gttagcgccc ccgaaagtcg
     7201 ctgacccgcc accagacgca gccgctcccc cgctcgccga tgtgccaccg ccgctgccga
     7261 attggccgga cgaaccgccc tcgccctgcg cgatgaatac ctgcggcgag cagccgacga
     7321 tcgccgacac acaccccaaa aaccctagcg aaccgtcccg taaagttctc atactcgaat
     7381 gaatgctcgg gcgagagtcg gccttcgaaa aaaatcgctc aaggcttcga cctggtcggc
     7441 tcggcacctg cggttggatg aagcacggca gttaggcgtt cctggtacaa gctgcccgga
     7501 gcgagtgttc gcaagcgctc cgcgcgcgct tcggcctcgg cacgacgacc gagtttcacc
     7561 aacgcggcca cgctgaggaa ctcgcgctcc aagcccagtg ctccgccgcc gaagtcgcgt
     7621 tcgtgctgcg ccagcatcac gagggcaccc gccgggtccg tcgacaagcg accgcgcgcc
     7681 agatcgagca aacgcgcttc gcgcaccaac tccgactccg gctcgctttt ggcgctggcg
     7741 gcaccgaccg atgctttggc ctctgcaacg gtcttgcgcg gcgcgggagc tccgatgtcg
     7801 ccggaccacg cgctcgccgg ggacgcgctt tgcagcgcgc tcggagccgc gctttctgac
     7861 gcgttcgagg cagcggcgct ttgcgaggcg ccggtcgcca ggggcgcgac tcttcgctgg
     7921 cgcgcaggag ctgcggcggc actgggagag ggcgccgaag ttcgctgatg taggagctcg
     7981 ggcagtgaca cgaccgctag cgcggcgctc ccgagcacgg cgccgagcgc tacgtgcttc
     8041 aaccacaaga acgctcccgc cgccgcgggc aaagccgaaa tctgggccag gcgccggcgc
     8101 gagcgctcac gggtcacggc gtcgagcgcg cccggcggct tcgcgtggcg gagcagcgcg
     8161 agcacgctct cgggtgcagc tttcgattcg agccagggcc gcgtatcgct catggtctgc
     8221 ctcttttttc ctgcttctcc atgcgctcga cggcgcgcgt gaactcggcg cgtgcggtcg
     8281 agagccgcga atagatcgtg cctacgggga cacccatgat gtcggcgatg gcgggcgccg
     8341 agatcccttc gatttcgaac atgacgaata ctgcacggcg ctcgagtccg agcgtttcga
     8401 gggcgagctc caagcgccga agcgcttcgc gctgtgcgag cacctgctct ggatctgccg
     8461 cgccatcgcg cagcagcgcc tcttcggggc cgaggcccaa ggtctcgtgt cggcgatgtg
     8521 cgcgtcgacg gtacgccgcc gccacgcgca aacagatgcc gaagagccac gtcgtgagct
     8581 gcgacgaatg atcgaaggtg gacaagcgct tgtgcaccac cagaaacacc tcttggagca
     8641 cgtcctccaa gtcctgctgg tgcactccca ggcgctgcaa cgtacgccac acgaaatcgg
     8701 cgtgcgcttc gtgcaccgca gccacatcgg ccggcatggc cggcgaagcg acgttggaga
     8761 gaggcgacgc gctggtcacg agagctgaat gccgggagct gacggaaacc ttcgaagatt
     8821 ctcgtaaact gccaaaaacg agagctcgtt cgctagctta gagcccgcga atggataggt
     8881 cagtcgcgag cgagccgaga ccgccgcgag gatgacgtgc ccagtcgcgg gcccttagcg
     8941 tgtctggaac gcgagcccgg ccaagcctag caccccgacc acgcggctct cgaacacggg
     9001 ttccgcgaca ccgcgctgca cgaattcggg gcggaccagc ggcacgaacg ccatcgcgcc
     9061 gagctcggcg cgaatccccg ccgcgagccc gaccgagagc tcgggcccga gcgacgcggc
     9121 cgcgaacacg cggtcacccg ggtcgagcgg cgtgagctcg cgcactaccg cgtgcatcgc
     9181 gccgacgccg agcgtcgcgc aagccgaaag cgcccagcg
//
LOCUS       DASTKS010000965         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1717296, whole genome shotgun sequence.
ACCESSION   DASTKS010000965 DASTKS010000000
VERSION     DASTKS010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717296"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2241)
                     /locus_tag="VFQ35_20385"
     CDS             complement(<1..2241)
                     /locus_tag="VFQ35_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933078.1"
                     /translation="MHARPNPVEALASLTRLKSTAEQRASWRQAIAALGHAAAGGFSG
                     APPLEGMAQDIIARAAEVAIDSGLADDLEWIAPGPATVALYEVTAALPPGKARRELGR
                     RVFARLYQGTASTFAAVAARMALTSARPFEPATLRARVSLLFDLPIGSNVNADPLALS
                     LVSRRELAERWVEQNKTGTMPARRLAAKLIEHAAREAVSRFHQGDSEPCDVLRSERLR
                     ATIQVLLADREPLVWRHVAVARGLLASVDNRLREEIEQSLDPALSPTEWRRGGVSLVA
                     LCAGDPDGALPSCRNLLAGPLLEVDRGLPAALVLGLPRVIEAEPNVAEELLERFALTQ
                     RPDVAEAVASLLADLRHPTFGQSAARSLRQTLMPRAEHQSSVLRAIAERAIRQLDRED
                     DDGDVLGSVRKALLAYEGLGARDAFEIATSAVKSLGRAMDFVSINDPHDEQVLPYVLG
                     TLSDIESVALERSRLSDLLLLGRRPGDTDVSVPEMDKLYDRLGRWILHAEGNPPAKSS
                     NAHAIGEQRRLRALLHLMDVDSTRQEDDKKVEARVCRALALLIERVKTGDGTQVHRIA
                     CAALARACDAAVREGIAEPADLLLLLAASHGAPRSFRAIAEASTHPDVRGVVHAYAEF
                     LTPAAFEGSEPPPEVLAIEANRQSSSEQSLRIAERVLRLSRGLGAGGSYRGESLRRIM
                     LRLGRALEQIARARGQTELFDSSGTGVDVLSDLEAALEDLQRLSRGARRRLLGEEGDI
                     AVVTEVP"
BASE COUNT          409 a          853 c          873 g          404 t
ORIGIN      
        1 cggcacttcg gtcacgaccg cgatgtcacc ctcttccccg agcaagcggc ggcgtgcgcc
       61 gcgcgacagt cgctgcaagt cttcgagcgc tgcctcgaga tccgaaagga cgtcgacacc
      121 ggtccccgaa ctgtcgaaga gctcggtctg gccgcgcgct cgggcgatct gttccagcgc
      181 tcggccgagc cgcagcatga tgcggcgtaa cgactcgccg cgataagagc ctccggcgcc
      241 caaacctcgg ctgagccgta agactcgctc cgcgatgcgc agcgactgct cgctcgagct
      301 ctgccggttt gcctcgatcg ccaacacttc cgggggaggc tcggagccct cgaatgccgc
      361 gggcgtgaga aactcggcgt aggcatgcac gacgccgcga acgtccgggt gcgtcgaggc
      421 ttcggcgatc gcgcggaagc tgcgtggagc gccgtggctc gccgcgagca agagcagcag
      481 atcggcgggc tcggcgatcc cctctcggac cgccgcgtcg caggcgcgtg cgagcgccgc
      541 acaggcgatt cgatgcactt gcgtgccgtc gccggtcttc acgcgctcga tgagcaacgc
      601 gagcgcgcgg cagactcgcg cttcgacctt cttgtcgtct tcctgccgtg tcgagtcgac
      661 gtccatcagg tgaagcagcg cgcggagccg gcgctgctcc ccgatggcgt gcgcgttcga
      721 gctcttggcg ggcgggttgc cttctgcatg caagatccag cggccgagtc ggtcgtagag
      781 cttgtccatt tcggggacgc tcacgtcggt gtcgccgggc cgccgcccga ggagcaagag
      841 atcgctcaag cgagagcgtt cgagcgcgac gctctcgatg tcactcaacg tgccgagcac
      901 gtaaggcaac acctgctcgt cgtgcgggtc gttgatggac acgaaatcca tcgcgcgccc
      961 gagggacttc actgcagaag tcgcgatctc gaaggcgtca cgcgcgccca agccctcgta
     1021 cgcgagcagc gccttacgca ccgagccgag gacgtcgccg tcgtcgtctt cccgatcgag
     1081 ctgtcgaatc gcccgctcgg caatggcacg gagcaccgag ctctgatgct cggcgcgcgg
     1141 catcagggtt tggcgcaagc tccgcgccgc ggattggccg aaggtcgggt gacgcagatc
     1201 ggcgagtaag ctcgcgaccg cttcggcgac gtcgggccgt tgcgtgagcg caaaacgctc
     1261 gagaagctct tcggccacgt tgggctcggc ttcgatgacg cgcggtagtc cgaggacgag
     1321 cgccgcgggc aacccgcggt ccacttcgag caggggtccc gccagcaagt ttcgacaact
     1381 cggcaaagcg ccgtcgggat cgcccgcgca caaggcgacc agcgacaccc cgccgcgccg
     1441 ccattcggtc ggagacagcg cggggtcgag cgactgctcg atctcttcac gcagccggtt
     1501 gtcgacgctg gcgagcaagc cgcgagctac cgcgacgtgg cgccagacga ggggctcgcg
     1561 atcggctagc agcacttgaa tcgtcgcgcg caggcgctca ctccggagca cgtcgcacgg
     1621 ctcggaatct ccctgatgaa agcgcgatac ggcctcgcgc gcggcgtgct cgatgagctt
     1681 cgccgctagt cgtcgcgccg gcatcgttcc cgtcttgttc tgctcgaccc agcgctcggc
     1741 gagctctcga cgactcacga gcgagagcgc cagcggatcc gcgttgacgt tcgaaccgat
     1801 cggcaaatcg aagagcaagc tcactcgcgc tcgcagcgtc gccggttcga aaggacgggc
     1861 ggaagtgagc gccatgcgcg ccgccaccgc cgcgaacgtg gaggcggtgc cctgatacaa
     1921 gcgcgcgaac acccgccgac cgagttcgcg gcgcgccttt ccgggcggga gcgcggccgt
     1981 gacttcgtag agcgcgacgg tcgcgggtcc cggcgcgatc cactcgaggt cgtcggcgag
     2041 gccgctgtcg atcgcgacct cggccgcgcg agcgatgatg tcctgagcca tgccttcgag
     2101 aggcggcgcg cccgagaacc cccccgcggc tgcgtggccc agcgccgcga tcgcttgacg
     2161 ccagctcgcg cgctgctccg ccgtcgattt gagtcgagtc agcgaggcga gggcctcgac
     2221 ggggttcggt cgcgcgtgca tcgatctccg tgagtttatt gggagtcgag ccgaagcgct
     2281 ctgtaattcc tcgggattgg ctcgatgatc gatgagagca ttggtgcgaa cgtgtgcgta
     2341 cctggcgcct cgagtggtgg ggcgcggagc cgttcgttct cgcgggtccg ggaacgcgag
     2401 ttcacacggt gtcgcgagag agccacggat cgcaggcaaa ttggctcgct gcgcgcctgg
     2461 tgcgctcctt gaaggtagcc ctcacgaaaa ccgcacggag gacagctcag catgtttcga
     2521 aggacgacct ggacccaca
//
LOCUS       DASTKS010000966         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1046895, whole genome shotgun sequence.
ACCESSION   DASTKS010000966 DASTKS010000000
VERSION     DASTKS010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046895"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1658)
                     /locus_tag="VFQ35_20390"
     CDS             complement(159..1658)
                     /locus_tag="VFQ35_20390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9933079.1"
                     /translation="MYIALVVVYAAILLVLCAYGVHRAHLAFSCLRHRERLAELTQRV
                     EVAEEQLPRVTIQLPLFNEATVARRLIEATGNMDYPRDRLEIQVLDDSTDETVAIAAA
                     AVQTLRARGIDAIYVRRADRKGYKAGALDYGLKTAKGELIAIFDADFVPQPSFLREVV
                     GHFQDPQIGMVQTRWDHLNRSASWLTSIQALMLDGHHLVENRARFANACMFNFSGTGG
                     IWRRAAIDAAGGWEHDTLTEDLDLSYRAQLAGYRFIYRADVCTPAELPEDMSAFRAQQ
                     YRWAKGTVQTARKLLGRVLRADIRAGARVEAAFHMLPHFAYPLTVLLTLVLLPALVFM
                     PATSLRTLLLVDVPLCLGATGSLATFYSMAEVAQNRSAWHAIKKLPGIIALGAGLAPH
                     LSLAVLDGLRSMAGEFVRTPKRGANQGRYRQYAKLPLGELFLALVSAASVIAAVATGH
                     WLAAPFASLFCVGYSSVAFLVVREQFVPAQAEPALASESDTANEMARAA"
     gene            complement(1860..>3126)
                     /locus_tag="VFQ35_20395"
     CDS             complement(1860..>3126)
                     /locus_tag="VFQ35_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933080.1"
                     /translation="PILAVALTLAAAAHAASPSDIAAARKLAQEGIELYDARDYQRSL
                     EQMQRAEALFDAPIHLLYIARAQQKLGHLVEASETYRKLVRFPVEAGASQTFKEAVES
                     GQRELPQVEARIGAIRIEVEPTSPEGLALSVDGKPVPLAVAGVDRPTDPGRHVVRAEA
                     KGFAPAEATVDLKDGEKRAVSLKLSPAGSAPANTINLVGASAESSPTPNIAPQSDRRV
                     SFFIGLRLGGAIPAGTLYNVQGERVATSDYISGGGFAELQGGVRFERYFALKLYLEGL
                     AFGAGSWLGNQDGESSVAGQGLGIGAMVGTKPGQWGGFGELGLGYRRFEVKREFGDGV
                     TCSGAQALGLNGLAFRLGGGAHIPVHRMIQLTPFVAASFGQVVSVHNDCEEYADTDKF
                     PASGSIGSGDRRGHQLFLIGIGGDLLLGL"
BASE COUNT          587 a         1042 c         1000 g          497 t
ORIGIN      
        1 ctctcgcgac gggcaggaac ttgggacacc ccctagtgcg gcgccggacg gcgcgcgccg
       61 aggcccaact cataacgagc gggcacttcg gtaaggggcg tcttcgggcc cagcgggcgc
      121 gcccaggtga gcacgtctgc gggccgtccg atcgcctttc acgccgcgcg agccatctcg
      181 ttcgcggtgt cgctctcgct cgccaacgcg ggctcggcct gagcgggcac gaactgctcg
      241 cgaacgacca ggaacgcaac cgaggagtac ccgacgcaga acagtgaagc gaagggcgcc
      301 gcgagccaat gccccgtagc aacggccgcg attacgctcg ccgcgctcac gagagccaag
      361 aacagctcgc cgagcgggag cttcgcatac tgacggtagc gaccttggtt tgctccgcgc
      421 ttcggggtgc gcacgaactc gcccgccatc gaccgcaagc cgtcgagcac cgcgaggctc
      481 aggtgcggag cgaggccagc gccgagggcg atgatgccgg gaagcttctt gatggcgtgc
      541 caggccgaac gattctgcgc gacctctgcc atcgagtaga acgtcgcgag cgagcccgtc
      601 gcgccgagac acagcgggac gtcgacgagc agcaaggtgc gcaagctggt cgccggcatg
      661 aacacgaggg ccggcaagag gaccagcgtc aggagtacgg tgagcggata ggcgaagtgc
      721 ggcagcatgt ggaaagccgc ttcgacgcgg gcaccggcac gaatatcggc acgaagtacc
      781 cgaccaagca gcttgcgcgc cgtttggacc gtgcccttgg cccagcgata ttgctgcgcg
      841 cgaaacgcag acatgtcttc cggaagctcc gccggagtgc acacgtccgc gcggtaaatg
      901 aagcggtacc ccgcgagctg cgcgcggtac gaaagatcga ggtcctccgt gagcgtgtcg
      961 tgctcccagc ctcccgctgc atcgatcgca gcgcgtcgcc agatgccgcc cgtgcccgag
     1021 aagttgaaca tgcaggcatt cgcgaagcgc gcgcgatttt ccaccaagtg atgcccgtca
     1081 agcatcaacg cttggatcga ggtgagccac gaagcgctgc gattcaggtg atcccagcgc
     1141 gtttgcacca tgccgatttg cgggtcctga aagtgaccga cgacctcgcg caaaaacgaa
     1201 ggctgcggaa cgaaatcagc gtcgaagatc gcgatcagct cgcctttggc cgtcttcagg
     1261 ccgtagtcga gcgcgccggc tttgtagccc ttgcgatcgg ctcggcgcac gtagatcgcg
     1321 tcgatgccgc gggcacgcag ggtttgaacc gcggccgccg cgatcgcgac ggtttcatcg
     1381 gtcgagtcgt cgaggacctg aatctcgaga cgatcgcgcg ggtaatccat gttccccgtc
     1441 gcctcgatca gccgccgcgc gacagtggcc tcgttgaaca gcggcagctg aatcgtcacc
     1501 cgcggcagtt gctcctccgc gacttcgacc cgctgggtga gctcggcgag ccgttcacgg
     1561 tggcgcaagc aggaaaaagc aaggtgggcg cgatgcacgc cgtaggcgca caacacgagg
     1621 aggatcgctg cgtagacgac taccagtgcg atgtacatgc tcgctttttt ccgtaacacc
     1681 ccacccattc acgattgtca cgcaaatgta attcgttgca cgatggtgta ggtgctcggt
     1741 cacccggccc cgtggacgca gggaaattgc gccgaaaact tgcccagaga acgctccgcg
     1801 cgtgttcgca atgccgcagc gtggccgggc tcgaagcgcc tcgccgaccg tcttagttgc
     1861 tacaagccga gcaggagatc gccgccgatc ccaatcaaaa acagctgatg cccgcggcga
     1921 tccccggagc caatcgagcc cgaggcgggg aacttgtcag tgtccgcgta ctcctcgcag
     1981 tcgttgtgaa cgctcacgac ctggccgaag ctggctgcca cgaacggagt cagctggatc
     2041 atccggtgga cggggatgtg agctccgcct cccaggcgaa acgcaagacc gttcaacccg
     2101 agcgcctgcg cgcccgagca cgtgactccg tcgccaaact cgcgcttcac ctcgaagcga
     2161 cggtatccca agccgagctc cccgaacccg ccccattggc ccggcttggt gccaaccatg
     2221 gctccgatgc caagcccctg ccctgcgacc gaactctccc cgtcctgatt gccgagccac
     2281 gagcccgcgc cgaacgcgag gccttccaag tagagtttca acgcgaagta gcgctcgaac
     2341 cggacgccgc cctgcagctc cgcgaagccg ccgcccgaaa tgtaatccga cgtcgcgacg
     2401 cgctcacctt ggacgttgta aagagtgccc gcggggatcg ccccgcccaa gcgcaatccg
     2461 atgaagaagc tgactcggcg atcggattga ggcgcgatgt tcggagtcgg actgctctcc
     2521 gccgatgctc cgaccaggtt tatcgtgttc gccggcgcac ttccggccgg tgagagcttg
     2581 agcgaaacgg cgcgcttctc cccgtccttc aaatcgacgg tggcctctgc gggagcgaaa
     2641 cccttcgcct ctgcgcgcac gacgtgacgt cccggatccg tcggacggtc gaccccggca
     2701 actgcgagcg gaaccggctt tccgtcgacg ctcagcgcga gcccctccgg ggacgttggc
     2761 tcgacctcga tgcggatggc accgatccgc gcctctacct ggggcaattc gcgctgtccc
     2821 gattcgacgg cctctttgaa cgtttgcgat gcgccggcct cgaccggaaa acgcaccagc
     2881 ttgcggtacg tctccgaagc ctcgaccagg tgaccgagct tctgctgcgc ccgcgcaatg
     2941 tagagcaagt gaatcggcgc gtcgaagagc gcttcggctc gctgcatctg ttcgagcgag
     3001 cgttggtagt cgcgtgcgtc gtagagctcg atgccctcct gggcgagctt gcgcgcggcc
     3061 gcgatatctg acggagaagc agcatgagcc gccgccgcga gagtaagcgc gaccgccagg
     3121 atagga
//
LOCUS       DASTKS010000967         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1495368, whole genome shotgun sequence.
ACCESSION   DASTKS010000967 DASTKS010000000
VERSION     DASTKS010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495368"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VFQ35_20400"
     CDS             complement(<1..944)
                     /locus_tag="VFQ35_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9933081.1"
                     /translation="MLLRNLKSALGVLALTALLASAPHSARAAGEASTEQSKLTIPFE
                     KLKLKNGLTLILHQDRSLPLVASNIWYHVGPANEPPKRSGFAHLFEHLMFEGSRHVGR
                     EFDTLLESVGATNVNGTTSWDRTNYFETVPREQLELLLWIESDRMGFMLDGLDQERLD
                     VQRDVVKNERRQTYENAPYGASSLLMLDSLFPEGTPYHGAIIGSMADLSNATLEDVRD
                     FFRTYYAPSNATLTLAGDFDLEEAKRLVQKYFETLPDRPREPSKFKPIAPPAQPIRRE
                     LTEPVELARVAFGHVTPPAYTPDDALLEVTMAVLAGGKA"
     gene            complement(1195..2220)
                     /locus_tag="VFQ35_20405"
     CDS             complement(1195..2220)
                     /locus_tag="VFQ35_20405"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HET9933082.1"
                     /translation="MPSRRQSTALLASLFCVSFSLEAAAHVSLDAPKSRYYSGNMADQ
                     NKLKYGPCGVSKDSRTTNTSLVTTFKPGEKVTVRWRETIQHPGFFRIAFDSDGQDFTF
                     PGSATAGDGVTILAEKIMDKSGAANLEYTYEVTLPNIECSNCTLQLVQVMTTDPPPYE
                     EGNGKDLYFNCADVILKGDGGGSGGAGGGGRGNGGMSSAGSAGEKNLGGSSGASAGGV
                     AGASAGGRGGQASGGQNTASGGSVGSGGAGSGGSPASGGTTGSGGATGQGGATTSGGA
                     ATSGGTAAASGGSTSAGTTGGGEANGCSCRVGHESSGATAPWIGAGLLALWRRRRLAK
                     RTASNQR"
     gene            complement(2394..>2790)
                     /locus_tag="VFQ35_20410"
     CDS             complement(2394..>2790)
                     /locus_tag="VFQ35_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9933083.1"
                     /translation="NATTDATRRELMPVARKYSLADVHAALCRFPLRPRERITIEYVL
                     LAGVNDSDADAERLAEFCEAFSHHINLIPFNDHAGSPFRAPSEARLNLFAKALLARRR
                     TVVSVRRSRGSDIQAACGQLARESQPPLL"
BASE COUNT          441 a          918 c          908 g          523 t
ORIGIN      
        1 gcttttccgc cagcgagcac cgccatggtc acctcgagca gcgcgtcgtc gggcgtgtaa
       61 gccgggggag tcacatgtcc gaaggcgacg cgcgcgagct cgacgggctc ggtgagctcg
      121 cgccggatgg gctgcgcggg cggtgcaatg ggcttgaatt tcgagggctc tcgcggacga
      181 tcgggcagcg tctcgaaata tttctgcacg agccgcttcg cctcttcgag gtcgaagtct
      241 ccggcaaggg tgagcgtggc attcgatggc gcgtagtacg tgcggaagaa gtcgcgcacg
      301 tcctcgagcg ttgcattcga tagatccgcc atcgagccga tgatggcgcc gtgatagggc
      361 gtgccctctg ggaacaggct gtccagcatc aagagcgagc tcgccccgta cggagcgttc
      421 tcgtacgttt gccggcgctc gttcttgacg acgtcgcgct gcacgtcgag tcgctcctgg
      481 tcgagcccgt cgagcatgaa gcccattcgg tcgctttcga tccagagcaa cagctcgagc
      541 tgctctcgcg ggaccgtctc gaagtagttc gtgcgatccc agctcgtcgt gccattgacg
      601 ttcgtcgcac cgaccgactc gagcagagta tcgaattccc gtccgacgtg ccgcgagcct
      661 tcgaacatca agtgctcgaa caggtgagcg aacccggagc gcttgggggg ctcgttcgcg
      721 ggacccacgt ggtaccaaat gttcgaggcg acgagcggca gcgagcgatc ctggtggaga
      781 atcagcgtga gcccattttt gagcttgagc ttctcgaagg ggatcgtgag cttgctctgc
      841 tccgtcgatg cctcgcctgc cgcacgcgcc gagtgcggcg cgctcgccag gagcgcagtg
      901 agcgcgagca cgccgagcgc gctcttgagg tttcgcagca acatccgctg caacctactt
      961 cacccgctcg cgaagggctc ctttttgaag gcgcagcttt tcggctcgct ggtcggagtt
     1021 gccgcactca aagccaatcc gaaatcgtgg accgagccct ccgaagatac gcgagctgtt
     1081 tggtgcgaga cgacgaggcg tacagaggtg aggattcgtc acgccgcgag caggtggaac
     1141 tccgtcactt cgatccacgg caaggtcgcc atgaatgggt tgaccgcaac ggattcagcg
     1201 ttggttcgag gccgtgcgct ttgctaggcg acgccgccgc cagagcgcga gcagtcccgc
     1261 gccgatccac ggcgccgtcg cgccgctcga ctcgtgaccg actcggcaag agcagccgtt
     1321 cgcttcgccc ccgccggtag tgccggccga ggtcgagccc ccgctcgccg cggcggtgcc
     1381 gccgctcgtg gccgcaccgc cgctcgtggt tgcgccgcct tggcctgtcg caccgcccga
     1441 gcccgtggtc cctcctgacg cggggctgcc ccccgagccg gctccgcccg agccaacgct
     1501 cccgccgctc gccgtgttct gcccgccgct tgcttgcccg ccacggccac cggcgctcgc
     1561 tccggccact cccccggcgc tcgctccgga gctgccgccg aggttcttct ctccggcgct
     1621 gcccgccgag ctcatcccgc cgttgccgcg gccaccaccg cctgcgcctc ccgacccgcc
     1681 accatcgccc ttgaggatga cgtcggcgca gttgaagtag aggtccttgc cgttgccttc
     1741 ttcgtagggc ggagggtccg tcgtcatcac ttgcacgagc tgaagggtgc agttcgagca
     1801 ctcgatgttc ggcaaggtca cctcgtaggt gtactcgagg ttggccgcgc cactcttgtc
     1861 catgatcttt tcggcgagga tggtgacacc gtctccggca gtcgcgctgc ccgggaacgt
     1921 gaagtcttgt ccgtcgctgt cgaacgcgat gcggaagaaa cccgggtgct gaatcgtctc
     1981 gcgccaccgc accgtgacct tctcgcccgg cttgaaggtg gtaacgagcg acgtgttggt
     2041 cgtgcgcgaa tccttcgaaa cgccgcacgg gccgtatttc agcttgttct gatcggccat
     2101 gttgccgctg tagtagcggg acttcggcgc gtcgagcgag acgtgggccg cggcttcgag
     2161 cgagaagctc acgcagaaga gggaagcgag cagcgcagta gactggcgtc gagacggcat
     2221 gtcggcatca acatttgtta tcaacgcgcg cgatgcaagc gctccgggtt agcatctatc
     2281 gatatcgtcg ccgctaccgc ccaggcacga attatcgctc gggattcgaa ccgccgcggc
     2341 gtctacggcg cggtgcggcg atgttttttg acctgtacga tcccgctcgc tcatcacagg
     2401 agcggcggct ggctctctct cgccagctgc ccgcaagcag cttgaatatc gctgccgcgc
     2461 gaccggcgca cactcacgac cgtacgcctt cgggccagga gcgctttggc gaagagattc
     2521 aaacgcgctt cgctcggcgc ccgaaacggc gagcctgcgt ggtcgttgaa cggaatcaaa
     2581 ttgatgtgat gcgaaaaggc ttcgcaaaac tccgcgaggc gctccgcgtc cgcgtccgag
     2641 tcgttgacgc ccgcgagaag cacgtactcg attgtgatgc gttctcgcgg cctgagcggg
     2701 aaccggcata gagccgcgtg cacgtcggcg agcgagtact tccgcgcgac tggcatcagc
     2761 tcccggcgcg tggcgtcggt ggttgcgttc
//
LOCUS       DASTKS010000968         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1942055, whole genome shotgun sequence.
ACCESSION   DASTKS010000968 DASTKS010000000
VERSION     DASTKS010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942055"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2493
                     /locus_tag="VFQ35_20415"
     CDS             <1..>2493
                     /locus_tag="VFQ35_20415"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9933084.1"
                     /translation="VGQASHYATAMCVDGYAVGLLVESHEGRPTKVEGNPDHPASLGA
                     AGALEQASLIDLYGAQRVREIRRRGVVSTPDTLAAALRSVFAPQRRGEGVHVVMPPTS
                     SEAHRSVLAELGMRMGASHVHFFTPQSRGNVWRGAQRAFGAVLEPRFDLRRAKVVLSV
                     DSDFLMHGPARLALARRFAEARRVTAPNDEMNRLYVVEAALSVTGQAADHRLALRDTD
                     LPRFVADLLVAVDTELGLSSSAIAGISTAVLRQANPVRGGFVRALARDLVAHRGSALV
                     LVGDRQPAALHAAAHALNDLLNPVETPVAYAPSPILQAGTSTHDSLGELSAALAAGAV
                     GALLSFDVNLLESTPGALAFDSLIRKAKTSFYFGTQQTAAADHCDWVAPLAHWLESWS
                     DARAFDGTASIGQPLLEPLYAASTLDEVLTLALGGEAPSARGRVRQALAASFGDDLEA
                     EWERSLRRGTVSGTSIAAVSRPALRWDWVAELIALATVAPVPLELSIYPDSRTLDGRF
                     ADNPWLVELPDPITKLTWQNAASLAPKTASRLGLENGDIAAISVGRVTREAPIIVVAG
                     QAEDSIGLSLGWGRASGLSSRGVNACSLQDLERLWTGSANLRPTGRKAELPITQSHQR
                     DEGFGEALFASSTLADYRRSPDFTKNQRKRQLSLYESDAPRARSQWGMAIDLNSCIGC
                     ASCVVACQAENNIPTVGAEGVMARREMHWLRIDRYVLPDATVINEPMACQHCEHAPCE
                     YVCPTAATVHSSDGLNQMVYNRCVGTRFCSNNCPYKVRRFNWFNYHADEPPLHSLVHN
                     PEVTVRARGVMEKCTYCVQRIENGKIRHKVKMA"
BASE COUNT          427 a          833 c          806 g          427 t
ORIGIN      
        1 agtcgggcaa gcatctcact acgcgaccgc gatgtgcgtc gacggctacg cggtagggct
       61 actcgttgaa tcccacgaag gacgccctac caaagtcgaa ggtaacccgg accatcccgc
      121 aagtttggga gcggcgggtg cgctcgagca ggcgtcgcta atcgacctct atggcgcgca
      181 gcgagtgcgt gaaattcgcc gacgcggagt cgtaagcacg cccgacacgc ttgccgccgc
      241 gttgcggagc gtctttgcgc cccagcgccg aggcgaaggc gtccacgtgg tgatgccgcc
      301 aacgagctcc gaggcgcacc gctcggtgtt ggccgagctc ggcatgagga tgggagcttc
      361 acacgtacac ttcttcacgc cgcagagccg cgggaacgtc tggcgcgggg cccaaagggc
      421 cttcggcgcg gtgctcgagc ctcgcttcga cttgaggagg gccaaggtgg tgctctcggt
      481 cgactccgac ttcttgatgc acgggcctgc gcgcttggcg ctcgcgcgtc gttttgcgga
      541 ggcgcggcgc gtcaccgccc ccaacgacga aatgaatcgc ttgtacgtcg tcgaggcagc
      601 gctcagcgtg accgggcaag cggcggacca tcgcctcgct ctacgagata ccgacctgcc
      661 tcgcttcgtc gccgacctac tcgtcgcggt ggacacggag cttgggctct cgagctcggc
      721 gattgcgggg atctcgacag cggttttgcg ccaagcgaat cccgtccgcg gtggcttcgt
      781 cagagccctc gcgcgcgatc tcgtcgctca tcgtggctcc gcgctcgtct tggtcggcga
      841 tcggcagccc gccgcgctgc acgccgccgc gcacgcgctg aacgacttgt tgaaccctgt
      901 cgaaacgccg gtcgcctacg cgccatcccc catcttgcag gcgggcacct cgacgcacga
      961 ctcattggga gagctcagcg ccgcactcgc ggccggggcg gtcggagcgc tgctctcgtt
     1021 cgacgtgaac cttctcgagt cgacgccagg tgcgctggcg ttcgattctc tgattcgcaa
     1081 ggcaaagacg agcttctatt tcggcaccca gcaaactgcg gccgcagacc actgcgactg
     1141 ggtcgcgccg ctcgcgcatt ggctcgagag ttggagtgac gcacgcgcat tcgacggcac
     1201 ggcgagcatc gggcagcctc tgctcgagcc tctatacgcc gcgagcacgc tagacgaggt
     1261 gctaacgctg gcgctcggtg gggaggctcc gtcggcacgc ggacgcgtgc gccaagccct
     1321 tgctgcaagc ttcggcgacg acctggaggc ggagtgggag cgttcgcttc gacggggcac
     1381 ggtcagcggc acttcgattg cagccgtctc tcgacccgcg cttcggtggg actgggtggc
     1441 ggagctcatc gcactcgcaa cggtcgcccc ggtcccgctc gagctctcca tttatcccga
     1501 cagccgcacc ctcgacggac gtttcgctga caatccctgg ctcgtcgagc tgcccgatcc
     1561 gatcaccaag ttgacgtggc agaacgcggc ctcgttggcg ccgaagacgg cgagtcgtct
     1621 tggcctcgaa aacggcgaca tcgcggccat atcggtcggc agggtcacgc gtgaagctcc
     1681 catcatcgtc gtggccggac aagcggaaga cagcattggg ctgagcctgg gttggggaag
     1741 agcgagcggc ctctcgagcc gcggcgtgaa cgcgtgctcc ctgcaagatc tggaacgact
     1801 ctggaccggc tccgcgaacc tgcgtcccac gggccgcaag gcagagctgc ccatcaccca
     1861 gtcgcaccag cgcgacgaag gctttggcga agcgctcttc gcctcgagca cgctcgccga
     1921 ttaccgacgc tcgcccgact tcaccaagaa ccagcgcaag cgacagctct cgctatacga
     1981 gtccgacgcg ccgcgcgcgc gaagccagtg ggggatggcg atcgatctca actcttgcat
     2041 cggctgcgcc tcctgcgtcg tcgcttgcca agccgaaaac aacatcccga ccgtcggcgc
     2101 cgagggcgtg atggctcgcc gcgagatgca ctggttgcgc atcgatcgct acgtcttgcc
     2161 cgacgcgacc gtcatcaacg agccgatggc ttgtcagcat tgcgagcacg cgccgtgcga
     2221 atacgtctgc ccgacggcgg cgacggtgca ttcgagcgat gggctcaacc agatggtcta
     2281 taaccgctgt gtcggcacac gcttttgctc gaacaattgc ccgtacaaag tccggcgctt
     2341 caattggttc aactatcacg cggacgaacc tccgctgcac tcgctcgttc acaatcccga
     2401 ggtgacggta cgggcgcgcg gcgtgatgga aaagtgcacg tactgcgtgc agcgcatcga
     2461 gaacggcaag atccgccaca aggtgaagat ggc
//
LOCUS       DASTKS010000969         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_154582, whole genome shotgun sequence.
ACCESSION   DASTKS010000969 DASTKS010000000
VERSION     DASTKS010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154582"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFQ35_20420"
     CDS             complement(<1..797)
                     /locus_tag="VFQ35_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET9933085.1"
                     /translation="MKGGAAPAGGGPKVIVIKKKAKGHGGGHGGAWKVAYADFVTAMM
                     ALFMVMWLLSQTDQETRQKLSEYFRTGMFSGAPSVLNGGSGIQDRAFFDVVGRSSMQP
                     ESAAFEQIALAVRARLQRTTDENPELKDLAKHVHVSVTEDGLLITIFDGGRDTVFDSS
                     SSELKPKLIELLEFLAPILGRLDNQIQLHGHTDARAFPKGSNRSNWQLSFERADAARA
                     VLERSGLRKGQVAGVFAHGDSALLNTKEPFAPENRRLAILAVRRGLE"
     gene            complement(794..1654)
                     /gene="motA"
                     /locus_tag="VFQ35_20425"
     CDS             complement(794..1654)
                     /gene="motA"
                     /locus_tag="VFQ35_20425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HET9933086.1"
                     /translation="MSTPLGLVIVFVSVIGGFLMAGGPLPVLIQPNELIVIGGAAAGS
                     LVISAPGRAMKRVTAAFKRGFGDHTPKKQDYLDLLKMLYQILALIRREGVLALESHVS
                     DRGSSSIFSAYPSVSKRHHAMDFLIDGLKQLVDGCSAEELSMLFDAELDTHHSEEHQP
                     INLIRNTGDALPGMGIVAAVLGIVITMAHLNGGPEEIGHHVAAALVGTFLGILLCYGV
                     VGPVANSIEAQSAAEGRYMSCIKEAVVAAARGVNPAIAIEFARRAIFSDERPTGPELE
                     KAFAELKGKS"
     gene            1794..2879
                     /gene="proB"
                     /locus_tag="VFQ35_20430"
     CDS             1794..2879
                     /gene="proB"
                     /locus_tag="VFQ35_20430"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET9933087.1"
                     /translation="MSRIELEKARRIVVKIGSRSLTGDGALIRRMAEEIAALRNDNRQ
                     VVLVSSGAIALGLSRLGYRTRPKEVGRLQAAAAAGQSVLMRRYDEAFAALGLTSAQVL
                     LTYSDLADRERLNNAREAFAALLDAGAIPIVNENDTVSTEEIRFGDNDQLASMVVPLV
                     GADALVLLTDVEGVLDARGRRIPEMAHDASVSVVSETRERVGTGGIQSKVDAARKATR
                     SGASAVIAPATRDGVLRELFSGADVGTFFPRRGPLRARKHWIAYTLRPRGTIVLDEGA
                     AQAMRAGRSSLLPVGVLGVRGDFNPGDAVRLVGPDGTEVARGLTHLGALDAARSAGKK
                     GPELALLFGSERSDLVVVHKDDLVVTD"
     gene            2956..3399
                     /locus_tag="VFQ35_20435"
     CDS             2956..3399
                     /locus_tag="VFQ35_20435"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9933088.1"
                     /translation="MSHSHHSRGHSDVNESAESHSCCSPPPLAAPAKVIDPVCGMTVN
                     PATAKGGSFEYQGTTYYFCNPRCRERFSANPESFLKKDEPAALQKAPPVAAPAGTIYI
                     CPMDPEVRATAPGPCPKCGMALEPLTVSVSDEPNPELVDMTRRFR"
BASE COUNT          581 a         1117 c         1141 g          602 t
ORIGIN      
        1 ttttcgagac cgcggcgcac ggccaagatg gcgaggcggc ggttttctgg agcgaagggc
       61 tctttggtgt tcaagagggc gctgtcgccg tgagcgaaga cgccggcgac ctgacctttg
      121 cggagtccgc tccgttcgag caccgcgcgg gcggcgtcgg cgcgctcgaa ggatagttgc
      181 cagttggagc ggttcgaacc tttcgggaag gcgcgggcgt cggtgtggcc gtgaagttgg
      241 atttggttgt cgaggcgacc gagtatgggc gccaagaact cgagtagctc gatgagcttg
      301 ggcttgagct ccgacgacga ggagtcgaag acggtgtcgc gcccgccgtc gaaaatggtg
      361 atgagcaagc cgtcttcggt aaccgagacg tgcacgtgct tcgcgagatc tttgagctcc
      421 gggttttcgt cggtggtgcg ctgcaagcgc gcgcgcactg cgagcgcgat ttgctcgaag
      481 gcggccgatt cgggctgcat gctggagcga cccacgacgt cgaagaacgc gcgatcttgg
      541 ataccggagc cgccgttgag cacgctgggc gcccccgaga acatgccggt tcggaagtat
      601 tcggagagtt tctggcgcgt ctcttggtcc gtttgggaca agagccacat caccatgaag
      661 agcgccatca tcgcggtcac gaagtctgcg taagcaacct tccaggcgcc gccatgaccg
      721 ccgccgtgac ctttggcctt cttcttgatg acgatgacct tggggccacc gccagccgga
      781 gccgctccgc ctttcatgac ttgcccttga gctcggcgaa ggctttctcg agctcgggac
      841 cggtcgggcg ctcatcggag aagatcgcgc ggcgcgcgaa ctcgatcgca atggcggggt
      901 ttacaccgcg cgcggccgcg acgacggctt ctttgatgca gctcatgtag cggccttccg
      961 cggccgattg agcctcgatg ctgttcgcga cggggccgac gacgccgtag cagaggagaa
     1021 tcccgagaaa ggttccgacg agcgccgcgg ccacgtgatg gccgatttct tcgggcccgc
     1081 cgttcaagtg ggccatcgtg atcacgatgc cgagcaccgc cgcgacgatt cccatgccgg
     1141 gcagcgcgtc gccggtgttg cggatgagat tgatgggctg gtgctcttcg gagtggtggg
     1201 tgtcgagctc ggcgtcgaac agcatcgaga gctcttcggc ggagcagcca tcgacgagct
     1261 gcttcaagcc gtcgatgagg aagtccatcg cgtgatggcg cttgctgacg ctcgggtaag
     1321 cggaaaagat gctggagctg cctcgatcgc tgacgtgcga ctcgagcgcg agcacaccct
     1381 cacgccgaat cagcgcgagg atttgataga gcatcttcaa gaggtcgaga taatcttgct
     1441 ttttgggggt gtggtcaccg aagccgcgct tgaacgccgc cgttacgcgc ttcatcgctc
     1501 ggcccggcgc cgagatcacg agggaccccg ccgccgcgcc gccgatcacg atgagctcgt
     1561 tcggctgaat gagcacgggt aaggggcctc cggccatcag gaatcctccg atgaccgaga
     1621 cgaagacgat gaccagccct aacggggtcg acacaaccct ttaggcggcc gggaagcgct
     1681 ttcctttagc ctcgcgcgtc ccacagaggg acagcaaagc gcgcgtacct ttcggtacgt
     1741 gcgccgcgac ggcctccccg ctccgccggc cgccgtgcta tccaacgccc gccgtgtcgc
     1801 gtatcgagct cgaaaaggcc cgccgaatcg tcgtaaagat tggctctcgc agcctcacgg
     1861 gtgacggcgc actcatccgg cgcatggccg aagagatcgc tgcgctccgc aacgacaatc
     1921 ggcaagtagt gctcgtctcg agcggcgcca tcgcgctcgg cctcagccgc ttgggctatc
     1981 gaacgcgtcc gaaggaagtg ggtcgcttgc aagcggcggc tgccgccgga cagagcgtac
     2041 tcatgcgtcg ctacgacgag gcgttcgccg cgctgggtct cacctccgcg caggtgctcc
     2101 tcacctacag cgatctcgcc gaccgcgagc gcttgaacaa cgcgcgcgag gcgttcgcgg
     2161 ccttgctcga cgccggcgcc attcccatcg tcaacgagaa cgacaccgtc tccaccgagg
     2221 agattcgctt cggcgacaac gatcagctcg cgtcgatggt ggtgccgctc gtcggcgccg
     2281 acgcgctcgt gttgctcacc gacgtcgaag gcgtgctcga cgcgcgcggc cgccgtatcc
     2341 cagagatggc gcacgacgcg agcgtctccg tcgtcagcga aacccgtgag cgcgtcggta
     2401 cgggcggcat tcaaagcaag gtcgacgcgg cccgcaaagc gacgcgctcg ggcgcttcgg
     2461 ccgtcatcgc ccccgcgacg cgcgacggcg ttttgcgcga gctcttctca ggcgccgatg
     2521 tcggcacctt ttttccgcgc cgtggcccgc tgcgagcgcg caagcactgg atcgcgtaca
     2581 cgctgcgtcc tcgcggcacc atcgtcctcg acgaaggcgc ggcgcaagcc atgcgcgccg
     2641 gcaggagcag cctgctcccg gtgggcgtgc tcggcgtccg cggtgacttc aaccccggcg
     2701 acgccgttcg cctggtgggg ccagatggca ccgaagtggc gcgcgggctc actcacctcg
     2761 gcgctctcga tgcggcgcgc tccgccggca aaaagggccc tgaactcgcg ctcctgtttg
     2821 gcagcgagcg cagcgatctc gtggtcgtgc acaaggacga cctagtcgtc accgactgac
     2881 cggcgtcacc ggggccgccc gcggttggca cgcaaggtgt aatgatgacg gcccaacgtc
     2941 gtcagccttg gtgccatgtc tcattcccat cactctcgcg gccactcgga cgtcaacgaa
     3001 agcgccgagt ctcactcctg ctgcagcccc cctccgcttg cggctcctgc gaaggtaatc
     3061 gaccccgttt gcggcatgac cgtgaacccc gccaccgcaa agggcggctc gttcgagtat
     3121 cagggcacga cttactactt ttgcaacccg cgttgccgtg aacgcttttc ggcgaacccc
     3181 gagagctttc tgaaaaagga cgagcccgcg gcgctgcaaa aagcgcctcc cgtcgcggcg
     3241 ccagcgggca ccatttacat ttgcccgatg gacccggaag tgcgcgcgac cgcgccgggg
     3301 ccttgtccta agtgcggcat ggcgctcgag ccgctcacgg tgagcgtcag tgacgagccg
     3361 aaccccgagc tcgtcgacat gacgcggcgc tttcgttgac gtccgagtgg ccgcgagagt
     3421 gatgggaatg agacatggca c
//
LOCUS       DASTKS010000970         7193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1717714, whole genome shotgun sequence.
ACCESSION   DASTKS010000970 DASTKS010000000
VERSION     DASTKS010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717714"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1394
                     /locus_tag="VFQ35_20440"
     CDS             282..1394
                     /locus_tag="VFQ35_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET9933089.1"
                     /translation="MKTRAAVAYAPGQPLVIETVDLEGPKAGEVLVEVKATGVCHTDE
                     FTRSGGDPEGLFPVIFGHEGAGIVREVGPGVTSVAPGDHVILLYTPECRACKSCLSRK
                     TNLCTAIRATQGKGVMPDGKSRFSIGGKMIHHYMGCSSFAEHTVLPEIAVAKVRKDAP
                     FDKICYIGCGVTTGVGAVIYTAKVEAGANVVVFGLGGIGLNVVQGARMVGADKIIGVD
                     LNPAREELARKLGLTHFVNPTQHGDNLVAHLVELTDGGADYSFECVGNVKLMRQALEC
                     CHRGWGVSVIIGVAGAGQEISTRPFQLVTGRVWKGTAFGGARGRTDVPKLVDWYMDRK
                     IEIDSLITHKLPLERINEAFDLMHEGKSIRTVIEYS"
     gene            complement(1414..2907)
                     /locus_tag="VFQ35_20445"
     CDS             complement(1414..2907)
                     /locus_tag="VFQ35_20445"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9933090.1"
                     /translation="MGWVEWLGGAACATVRRMHELDRELERAFSPEGFRDLGHTLVDR
                     LADYLELAARRGQPVLPSARPEELARGLESDFVEPNEAVWSDRVPRWLDGAIHLHHPR
                     YVGHQVTSPLPEAALSELMAALTNNGAAVYEMGPLEVAMERALISRMSRWIGFGEGAD
                     GVFTSGGSLGNLTALLAARQAKAGFDAWSDGLSGGPSLGVLVSEQAHYSAQRALQIAG
                     LGKASVETVPVDPSFRMRPELLAEAQRNLEARGRRAIAVIASAGSTATGAYDPLEPIA
                     AYCEREGLWLHVDGAHGASALLSERLRPRLAGVERADSVVWDAHKMLLMPALVTAVVF
                     RNGDRSYEAFAQSASYLFEGDDPRSEWFNLATRTLECTKRWMVLPLYTALCRYGAGFF
                     GRYVEAQVDLAARFAEAIQTSGDFELLVKPESNIVCFRYVGAGERNTAELNALQRRIR
                     NEVLRSGRYYLVQTTLRGAVYLRTTIINPLTSDADLSELLQTLRDAV"
     gene            2973..3980
                     /locus_tag="VFQ35_20450"
     CDS             2973..3980
                     /locus_tag="VFQ35_20450"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9933091.1"
                     /translation="MAMETAVILAGAVAKGAFEAGAIEVLAPHAEELGITRVVGASAG
                     ALNAALLAIALHSRAEVEVSKRLVELWSDRATWHNVLDLKLYDIWSRTGLGTADRVLD
                     LMVRAVKGVHSTDPRPLALALVVTALRGSAGNIGGVSATSFEGVVRFENAVFDDPKER
                     ERIFKTALASAAFPVLFAPVEVPGVGLCIDGGAVNNAPVRLALDGSGVDRVIVISPEP
                     LLISPPEPLSGMNLLGHVAEILINERLYRDLHDAEAVNGILKKLEGLSALGFSDAEVQ
                     QVKATLGWRKLEIVQIRPPEKLSGNAFEAFSDQSRRFEYIEAGRRAALAQLGALVPSR
                     G"
     gene            4309..4725
                     /locus_tag="VFQ35_20455"
     CDS             4309..4725
                     /locus_tag="VFQ35_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933092.1"
                     /translation="MIAYLRSLSAPKMLLWCYLVWYLFVVVTYFDPKPRLWVSSLGLS
                     IIVGTGLYLSTAYASRQRTPLSFWQIFRFYLMPFCVSSFSALIKGRGFVLIFHPTLEK
                     NVEALALCAGFCGLVLACKNLGVSSQKSASPTFERV"
     gene            complement(4777..5592)
                     /locus_tag="VFQ35_20460"
     CDS             complement(4777..5592)
                     /locus_tag="VFQ35_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933093.1"
                     /translation="MKSRFLRPPVVLCALTSLLIASACEDDKSSNCYVAGAPNLGQGG
                     SNNSGGSTNTGGVAPTAGQPSETSGGAAGAAGMGALGGAGPSAGGAGNEAGSGPAEGG
                     ASALPANCAGKACTYDCSAEGSCAEKIDCPPGFDCVVSCGRDACAGGIACGTGTNACK
                     VTCSGQGSCAKEVECAGNNGCNVACSGQASCAAGVRGSNGAKIQVDCTGQNSCKGGVI
                     ENGPDVLINCTGQNSCDGFVQCNTNRCEVHCSADSCDSKVQCNAGQRLGNCPR"
     gene            5992..>7193
                     /locus_tag="VFQ35_20465"
     CDS             5992..>7193
                     /locus_tag="VFQ35_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933094.1"
                     /translation="MRTEEERVADAGELMQTFIERTRIGSNGPQRRYLWTDAFALCNL
                     LALARATSDARYEAQALELVEGVHRTLGRFRTDDTRRGWISGLPDEDALSHPTRGGLR
                     IGKPLPERAAHETFDEELEWERDGQYFHYLTKWMHALDQLARERREPRYNEWARELAE
                     TACRAFCRANPGGRCGVVWKMSTDLSRPQVSSLGAHDPLDGFVTVSQLRASAAGWHGV
                     TTPRLNNELRELAGMLDETSWATPDALGLGGLLIDACRLAQLIESYSLDVADLLDRVL
                     AEIVAGMKLYVSRAEFERPGWQRLAFRELGLVIGLSGLRYLAPAKRTLRRAIEQYSYL
                     QPLLLDFWLEPAQRLTPSWAEHRDINEVMLATSLVPEGVLDLGAPEVHATGDFDFSSG
                     PGGTVSTGT"
BASE COUNT         1195 a         2309 c         2364 g         1325 t
ORIGIN      
        1 cccgacacga cgaacacgaa ctcgtgctgt gcctgatcgt accaaaattc cgggggcgag
       61 ctctggccgt gcgagacgat gcgctcgaac ttcacggcac gacgcgcgac gagaagctcg
      121 ctgatctcga cttcgcgctc gtcgggcaag ttttcgagca ggcttccgcg ttgcatcacg
      181 ggagcatact tcgcgagacg gggctcagcg aaatcagcgg atttcggcga gctcggcgga
      241 acgattccat tggagcccgg gcagggctac cctgctccgc catgaagact cgcgccgccg
      301 tcgcctacgc tcccggtcaa cctctcgtca tcgaaaccgt cgacctggaa gggccgaagg
      361 cgggtgaggt gctcgtcgag gtgaaggcga ccggagtctg ccacaccgac gaattcactc
      421 gttcgggggg cgatccggaa gggctgtttc cggtcatctt cgggcatgag ggggcgggca
      481 tcgtccgtga ggtcggcccg ggtgtaacga gtgtcgcgcc tggcgatcac gtgattttgc
      541 tctacacgcc ggagtgtcgc gcttgcaaat cgtgcttgag ccgcaaaacg aacctctgca
      601 ccgcgattcg cgcgacgcaa ggcaagggag tgatgccgga cggcaagagt cgcttctcga
      661 tcggtgggaa gatgattcac cattacatgg gctgttcgag cttcgcagag cacacggtgc
      721 tgccggagat cgcggtcgcg aaggtgcgta aggacgcgcc gttcgacaag atttgttaca
      781 tcggttgcgg ggtgactacc ggcgtcggag ccgtgattta caccgcgaag gtcgaggcgg
      841 gcgccaacgt ggtcgtcttc ggcctgggcg gcatcgggct caacgtggtc caaggcgcgc
      901 gcatggtcgg tgccgacaag atcatcggcg tcgacctcaa cccggcgcgc gaggagctcg
      961 cgaggaagct cgggctcacg catttcgtca atcccaccca gcacggagac aacttggtgg
     1021 cgcacctcgt cgagctgacg gacggcggcg ccgactacag cttcgagtgc gtgggaaacg
     1081 tgaagctcat gcgccaagcg ctcgagtgct gccatcgcgg ctggggtgtc agcgtgatca
     1141 tcggcgtcgc cggcgcgggc caggagattt cgacgcggcc gttccagctc gtgacgggcc
     1201 gcgtttggaa gggcacggct tttggcgggg cgaggggtcg caccgacgtg ccgaagctcg
     1261 tcgactggta catggaccgc aagatcgaga tcgacagcct catcacgcac aagctcccgc
     1321 tcgagcgcat caacgaagcg ttcgacctga tgcacgaagg caaatcgatc cgtaccgtga
     1381 tcgagtactc gtgatacgcg gccgctccgc tttctagacc gcgtctcgca gcgtttgcaa
     1441 caactcgctc aggtcggcgt cgctggtcag tggattgatg atggtggtgc gcaggtagac
     1501 ggccccgcgc agggtcgtct gcaccaagta gtagcggccg gagcgcagca cttcgttgcg
     1561 gatgcgccgt tgcagcgcgt tgagctcggc cgtgtttcgt tcccctgcgc cgacgtagcg
     1621 gaaacatacg atgttcgact cgggcttcac caagagctcg aagtcgccac tcgtctggat
     1681 cgcttccgcg aagcgcgcgg cgagatcgac ttgcgcctcg acgtagcgcc cgaaaaaacc
     1741 agcgccgtag cggcacagcg ccgtgtagag cggcagcacc atccagcgct tggtgcactc
     1801 gagggtgcgg gtcgccaggt tgaaccactc cgagcgcgga tcgtcgccct cgaatagata
     1861 cgacgcgctc tgcgcgaaag cttcatagga gcggtcgccg tttcgaaaca cgaccgcggt
     1921 cacgagcgcc ggcatgagca acatcttgtg ggcgtcccag actacgctgt ccgcgcgctc
     1981 cacccccgca agccggggac gaaggcgttc ggagagcagc gcgctcgcgc cgtgggcgcc
     2041 gtcgacgtga agccagaggc cttcgcgctc gcagtacgcg gcgatgggct cgagggggtc
     2101 gtaagcgccc gtcgcggtag agccggcgct cgcgattacg gcgatcgcgc gccgcccccg
     2161 cgcctcgagg tttcgctgtg cttcagcgag gagctcgggg cgcatccgga agctcggatc
     2221 gaccggcacg gtctcgacgc tcgccttacc gagccccgcg atttggagcg cacgctgcgc
     2281 cgagtagtgc gcctgttcag aaaccaagac tccgagcgat ggtcctccgc tcaatccgtc
     2341 gctccatgcg tcgaaccccg ctttggcttg acgcgcggcg aggagcgccg tcaggttccc
     2401 gagtgagccg ccggaagtaa acactccgtc cgcgccttcg ccaaaaccaa tccagcgtga
     2461 catgcgggag atgagcgcgc gctccatcgc cacttcgagc ggccccatct catagaccgc
     2521 ggcgccgttg ttggtgagcg cagccatcag ctccgagagg gcggcctcgg gcagggggct
     2581 cgtcacctga tggcctacgt accgcgggtg atgcaagtgg atcgcgccgt cgagccagcg
     2641 tggcacgcgg tcggaccaaa ccgcctcatt gggttcgacg aagtcgctct ccaacccgcg
     2701 ggcgagctct tcgggacggg ccgacggcaa caccggctgt ccacggcgcg cggcgagttc
     2761 gaggtagtct gccaggcgat ccaccaacgt gtgaccgaga tctcggaacc cttccggcga
     2821 gaacgctctc tcaagttcac ggtcgagctc gtgcatgcgc cgaacggtcg cacacgctgc
     2881 accgccgagc cactcgaccc agcccattcc cttgggaccg gggactcgag gctcacgttg
     2941 ccgccctcgc caggccgaag tatgcacctt tcatggcgat ggaaacggcg gtcattctcg
     3001 cgggcgcggt cgctaagggt gcgttcgagg cgggcgcgat cgaggtcttg gcgccgcacg
     3061 ccgaagagct cggcatcacg cgagtcgtgg gcgcgagcgc tggcgcgttg aatgcggcgc
     3121 tgctcgcgat cgcgcttcat tcgcgtgccg aagtcgaggt cagcaaacgc ctggtcgagc
     3181 tctggtcgga tcgagcgacg tggcacaacg tactcgatct caagctctac gacatttggt
     3241 cgcgaacggg cctcggcacc gccgaccgcg tgctcgatct gatggtgcgt gcggtgaagg
     3301 gcgtgcactc gacggatccc cggccgctcg ctcttgcgct cgtcgtgacg gcgcttcgcg
     3361 gtagcgccgg caacatcggc ggggtgagcg cgaccagctt cgaaggtgtg gtgcgcttcg
     3421 agaatgcggt gttcgacgac cccaaggagc gtgagcgaat cttcaagacc gcgctcgcct
     3481 ccgccgcctt tccggttctg tttgctccgg tagaggttcc gggcgttggg ctctgtatcg
     3541 atggtggcgc cgtgaacaac gcccccgttc gcttggccct cgatgggagc ggagtcgatc
     3601 gcgtcatcgt catttcgccc gagccgttgc tgatcagccc accggagccg ctctcgggta
     3661 tgaatctgct cggccacgtc gccgaaatcc tcatcaacga gcggctctat cgagatctgc
     3721 acgacgccga ggcggtgaac ggcatcctga agaagctcga agggctttca gcgctcggct
     3781 tctcggacgc tgaggtgcag caggtgaagg cgacgctcgg gtggcgcaag ctcgaaatcg
     3841 ttcagattcg tccgcccgag aagctctcgg gcaacgcttt cgaggcgttc agcgaccagt
     3901 cgcgtcggtt cgagtacatc gaggctgggc gtcgcgccgc gctcgcacag cttggcgcgc
     3961 tggttccgtc gcggggctag ggggcgtccc aagttccgcg ccctgctgcg agccaagatc
     4021 ctgcggctgc aaagcacggc aatcgtcctc cgctcctcga aaggcttcgg ccttgctccg
     4081 gtgctcactc ctcggggcgc gcttctcgct cgcgtttctt ggctctcacg acgggcagga
     4141 acttgggaca cccccccagc tctcccacgg tgacggctcc cgccgacgca tgcggagcgc
     4201 gcctctcatg cgcgggaagc gctttctgca agggctcccg gctgctgctc cctcgttcgc
     4261 agcattcgtg aaaactctgc gacacggagg gcgcggcatg ctccccgcgt gatcgcttat
     4321 cttcgctcgt tgtctgcgcc gaagatgctg ctctggtgct acctcgtctg gtacctgttc
     4381 gtggtcgtca cgtatttcga cccgaagccc aggttgtggg tgagctcgct cggactcagc
     4441 atcatcgtgg ggacgggcct gtacttgagc acggcgtacg ctagccggca gcgcacgccg
     4501 ctctcgtttt ggcaaatctt caggttttac ctgatgccgt tttgcgtctc gagcttctcg
     4561 gccctgatca aaggccgggg cttcgtgctg atctttcacc cgaccctgga gaagaacgtc
     4621 gaggcgctcg ctctttgcgc cggtttttgt ggcctcgtcc tcgcttgcaa aaacctcggc
     4681 gtgagctcgc aaaaaagcgc gagccccacc ttcgagcgag tctagctcga tgacggccgc
     4741 gtcgatcggc gcgcggccgt cacctcgcta atgacatcag cgtggacagt tgccgaggcg
     4801 ttgcccggcg ttgcactgca ccttggagtc gcaggagtcg gcgctgcagt ggacttcgca
     4861 gcggttcgtg ttgcactgaa cgaaaccgtc gcacgagttc tgaccggtgc agttgatgag
     4921 cacgtcggga ccgttctcga tgacgccgcc cttgcacgag ttctgtccag tgcagtccac
     4981 ttgaatcttg gcgccgttcg agccgcgcac gccggctgcg cacgatgctt gcccgctgca
     5041 ggccacgttg catccgttgt tgcccgcgca ttctacttct ttggcgcaag aaccctggcc
     5101 cgagcacgtc accttgcatg cattcgtccc ggttccgcag gcgatgcctc ccgcgcaagc
     5161 gtcgcgaccg cagctcacga cacaatcgaa gcctggcgga cagtcgatct tctccgcgca
     5221 cgagccctcg gcgctgcagt cgtaagtgca ggccttgccc gcgcagttgg cagggagtgc
     5281 agaagctccg ccttcggcgg ggccactccc agcttcgttg cctgctccac ccgcgcttgg
     5341 tccagcgccg ccgagcgcgc ccatgccggc agcgcctgca gctccgcctg acgtttcgga
     5401 tggctgccca gcggttggcg cgacgccgcc cgtgttggtc gaaccgcctg agttgttgct
     5461 gccgccctgt cccaggttcg gggcaccggc gacgtagcag ttggagctct tgtcgtcttc
     5521 gcaagcactt gcaatgagca gactcgtgag agcgcagagg actacgggag gcctaagaaa
     5581 tcgggatttc atgagcggag cgcctttcgt gggttcggcg cgcgcaccat aaggaggagc
     5641 gctttcaaag tcagctacaa tccgtcatgg acagaacgcg cccgatcaag tggtctgaaa
     5701 aagttccaac ttcgcgcgcg aaatggtcct ctccggtcct cccgcggtcg ctgtacgcaa
     5761 cctgcccagg gtgactgtcg tcatcagccg ggcgagtgag cgcaagcatt gcaggccgtc
     5821 ctgcagccga tgcgggtcgc ggctcgcttt ccgcgaactc ccttctgggt ttccctcgcg
     5881 ctggccagag catgcgtgac tcctcgcggg ggagccttcg cgcgcgcgtc gaatcgtgtg
     5941 ctcgcggtgc gcgctcgtcg agcggcacga cgcatgcagc ttcgcgtggt catgaggacc
     6001 gaggaggaac gcgtcgccga cgcgggggag ttgatgcaga ctttcatcga gcgcactcgc
     6061 atcggctcca atggtccgca gcggcgctac ttgtggacgg acgcctttgc tctgtgcaat
     6121 ctcctcgcgc tcgcgcgagc gacgagcgac gcgcgctacg aggctcaggc actcgagttg
     6181 gtggagggcg tgcatcgtac gctcgggcga ttccggaccg acgacacgcg acgagggtgg
     6241 atcagcgggc tacccgacga ggacgccttg tcgcacccaa ctcgcggcgg gttgcgaatc
     6301 ggcaagcctc tgcccgagcg cgctgctcac gagaccttcg acgaagaact cgagtgggaa
     6361 cgcgacggcc aatacttcca ctatttgacg aagtggatgc acgctctcga ccagctggcg
     6421 cgagagcgcc gagagcctcg ctacaatgaa tgggcgcgtg aactcgccga gaccgcttgc
     6481 cgcgctttct gcagagccaa tcctggggga aggtgtggcg tcgtctggaa aatgagcacc
     6541 gacctttcgc ggccacaggt gtccagcttg ggcgctcacg atccgttgga cggcttcgtt
     6601 accgtgtcgc agcttcgcgc cagcgcggct gggtggcacg gagtgacgac tccgcgcttg
     6661 aacaacgaat tgcgagagct cgcgggtatg ctcgacgaga ccagctgggc gactccggac
     6721 gcgctcgggc tcggcggtct cttgatcgac gcgtgtcgtt tggcgcagct catcgagagc
     6781 tactcgctgg acgtcgccga cctcttggat cgcgtcctgg cggaaatcgt cgcaggaatg
     6841 aagctctacg tgagccgtgc ggagttcgag cggcccggat ggcaaaggtt ggcttttcgc
     6901 gagctgggtc tcgtcatagg cctgtccgga ttgcgttact tggctccggc gaagcgcacg
     6961 ctccgacgcg ccatcgagca gtactcctac ctgcagccgc tcctgctcga tttctggttg
     7021 gaacccgccc agcgactaac accgagctgg gccgagcacc gagacatcaa cgaagtcatg
     7081 ctcgcgacca gcctcgtacc ggaaggagtg ctcgacctcg gagcgcccga ggttcacgcg
     7141 acgggcgatt tcgacttctc gtcgggcccc ggcggcacgg tgagcacggg cac
//
LOCUS       DASTKS010000971         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1495780, whole genome shotgun sequence.
ACCESSION   DASTKS010000971 DASTKS010000000
VERSION     DASTKS010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495780"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFQ35_20470"
     CDS             complement(<1..465)
                     /locus_tag="VFQ35_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933095.1"
                     /translation="MRSGTVLVVDDDILLASTLLRALTTFGWEVEVAHSGNTALELMS
                     QRAFDTLVLDLLIPDTDGFAVLDRLREFPAEKRPATILLSGHLDVPSTVRAVRAGAFD
                     VLEKPVSGALLHQRLLAALAVAGRRHALTAKEAEILSRIGGESAAIHQVCDQV"
     gene            1003..2232
                     /locus_tag="VFQ35_20475"
     CDS             1003..2232
                     /locus_tag="VFQ35_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933096.1"
                     /translation="MRVESLRCWKLASALAGVLPLTVASHAAAQAVPDCSTLTNPVYV
                     TGSSAVKPFLGAVATELVSLTGADKISVVYISKGSCDGVNALATAGTLVTGTASVWGT
                     DGKEVAGGCNLPTVGVAADIGVSDVFAASCGVNALPAGVKDFLGPVQSMTFAVPKKST
                     QTAISAEAAYLTFGLGAAGETPWSDPTKYFVRTAASGTQQMLSAAIGVPAAKWAAPTA
                     NQLGGSGAVRDAINMVQSTGSQADADLTIGILATDVVDKSRATLRALPYQHFGQTCGF
                     LPDSTAESFDKQNTRDGHYMVWGPLHLITRVTGGVATNASARKVIDILTGAVVPSFDL
                     IALEATTGVVPDCAMRVARDSEVGPLSSYMPPHSCECKFVAAATSKTPEGCTPCTAPT
                     ECPSSRPACNYGFCEVR"
     gene            2235..2762
                     /locus_tag="VFQ35_20480"
     CDS             2235..2762
                     /locus_tag="VFQ35_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933097.1"
                     /translation="MTRKLHTMAALAAFATLFYVGCGDDDENVSPNTGGKTGSGGKTG
                     SGGANNAGTSSGSGGKSAGSGGKSSANGGNANPGGETSTGGVAPSEGGAGGVPVTPEG
                     GSAGAGGAPEMMPPCSSATYDATAECYAACTPAKTSTSEQFLNHCSEAVGVQCTKFTA
                     PLPKLGANGALPPLP"
     gene            2891..>3327
                     /locus_tag="VFQ35_20485"
     CDS             2891..>3327
                     /locus_tag="VFQ35_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933098.1"
                     /translation="MVRFPLLNRVLFVGSGVLLCALPARAQAPAAQTDAPAAQTEPAA
                     PDDAEIPAAPQPAPAPVSPTAPPAEIPVKAPPAFASSPVPAVDAPPSSATAGASTEPK
                     PPGASPLVDIAGYIQGEYQSHQDSEDQLRQGGSLLNQNRFLIRR"
BASE COUNT          587 a         1126 c         1053 g          561 t
ORIGIN      
        1 cacctggtcg cacacctggt gaatcgctgc gctctcgccc ccgatgcgcg agagaatctc
       61 cgcttccttc gcggtcagag cgtgacgacg ccccgcgacc gcgagcgcgg caagcaggcg
      121 ttgatgtaag agcgcgcccg ataccggctt ttccagcacg tcgaacgccc ccgcgcgcac
      181 cgcgcgcacg gtggacggca catccaagtg cccggacaag agaatcgtag cgggccgctt
      241 ttcagcagga aactcgcgca accggtccag aaccgcgaat ccgtcggtgt cgggtatcaa
      301 gaggtcgagc acgagcgtat cgaaggcgcg ttggctcatg agctcgagcg ccgtgttgcc
      361 gctgtgagct acctccacct cccacccgaa tgtcgtgagc gcgcgcagca aagtgctggc
      421 caaaagaata tcgtcatcga caaccaggac ggttcccgac ctcatttctt cccttcctct
      481 cgccgcgagg cggtaccccc agcggctaat cgcgaagcat gcagccaggc tctttctcct
      541 gtcaatcccg cattgcgggt cgatcctcaa aagtgaggag tcgaaagaat ttgcttgcgc
      601 gtcgaaagcc tcaaaaatac gcgacaattt tgcggaggct caggtttttc gccgggccat
      661 gctccgcttc cgcttcgatg cggcggagga gccgggcgcc gaacgtcggt atcgcttcgc
      721 acgtgcgctc cgcgcgtgat ctcgggagct catagcggtc gagcgccgag agcaggcccc
      781 gagtatcgcg cctcgaggta tcgcgtctcg aggcgtttcg atgacgaacc gcgatcgcgc
      841 gggtgacgaa cgcgcccgac tcgcgactta gaagcttcgc gcgacaactc gtcagggtga
      901 tgcgtcacca aaatatcacg ggttggtcgc gagcgtgacg cgccccaatc ctagatacgc
      961 cgtcgcgctt cgctttgctc gaagtgtcga aaggaaatag cgatgagagt tgaatcgctt
     1021 cgttgctgga agctcgcgag cgcgctcgcc ggcgtcttgc ctttgacggt cgcgtcacac
     1081 gccgccgccc aggccgtgcc tgactgttct acgctcacga acccggtgta cgtgacggga
     1141 tcctccgcgg taaagccgtt tctcggggcg gtcgccaccg agctcgtcag cttgacgggc
     1201 gcggacaaga tcagcgtcgt ctacatcagc aagggctcgt gcgacggcgt gaacgcgctc
     1261 gcgactgccg gcacgctcgt cacgggaact gcgagtgtgt gggggaccga cggcaaggag
     1321 gttgcgggtg gctgcaactt gccgacggtt ggcgttgcgg ccgacatcgg cgtgtccgac
     1381 gtcttcgcgg cgtcgtgcgg cgtgaacgcg cttccggccg gagtgaagga cttcctcggc
     1441 ccggtgcaat cgatgacctt cgcggtgccg aagaagtcga cgcaaaccgc gatcagcgcg
     1501 gaagctgcgt acctcacctt cggcctcggc gccgccggcg agactccgtg gagcgacccg
     1561 acgaagtact tcgtgcgtac ggccgcgagc ggcactcagc agatgttgtc cgccgcgatc
     1621 ggcgtaccgg ctgcaaaatg ggcggctcca accgccaacc agctcggcgg ttcgggcgcc
     1681 gttcgcgacg cgatcaacat ggtccagagc accggctcgc aagcggacgc cgacctcacg
     1741 atcggcatcc tcgcgaccga cgtggtcgac aagagtcgcg ccacgcttcg cgcgctgccc
     1801 tatcagcact tcggccaaac ctgcgggttc ttgccggact ccaccgcgga gtcgttcgac
     1861 aagcagaata cgcgcgacgg gcactacatg gtttggggac cgcttcatct catcacgcgc
     1921 gtcacgggcg gcgtcgctac gaacgccagc gcgcgcaagg tcatcgacat cctcacggga
     1981 gcagtcgttc cgagcttcga tctaatcgcc ctcgaggcga ccaccggcgt cgttcccgat
     2041 tgcgccatgc gtgttgcgcg cgacagcgaa gttggccccc tctcgagcta catgccgccg
     2101 cactcgtgcg agtgcaaatt cgtcgcggcc gcgacgagca agacccctga aggctgcact
     2161 ccctgcaccg cgcccaccga gtgtccgagc tcacgtcccg cctgcaacta cggcttctgc
     2221 gaggttcgct gaaaatgacg agaaaactac acacgatggc cgcgctcgcg gcttttgcga
     2281 ccctctttta tgtcgggtgc ggtgacgacg acgaaaacgt gtcaccgaac accggcggca
     2341 agaccgggag cggcggcaag accgggagcg gcggcgcgaa caacgccgga acgagctccg
     2401 gtagcggtgg caagtcagcg ggcagcggtg gcaagtcttc cgcgaacggc ggcaacgcaa
     2461 acccgggcgg cgaaacgagc acgggcggcg tcgctccgag cgaaggcggc gccggcggcg
     2521 tgccagtcac tccggaaggc ggttcggcag gtgcaggtgg cgcacccgag atgatgcctc
     2581 cgtgcagctc ggccacttac gatgcgaccg ctgagtgcta cgcggcctgt actcctgcca
     2641 agacgtcgac gtccgagcag ttcttgaacc actgctccga ggcggtcggc gtgcaatgca
     2701 ccaagttcac cgcgccgctc cccaagctcg gcgcgaacgg cgccttaccg ccgctgccct
     2761 gagcatcgtc gcgtagaccc accgcttttc cgttcggctc cgctcgtgag cgagtcgcca
     2821 ccgaggcgcc ctcgcgaatg gacggagccg cgcggtagcg ggctcactct tttcagggat
     2881 ttgaaacatc gtggttaggt tcccgttact gaatcgtgtt ctcttcgtgg gctcgggcgt
     2941 gcttctctgt gctctccctg cgcgcgccca agcgcctgcc gcgcagaccg acgcgcccgc
     3001 tgcacagacc gagccggctg ctcccgacga cgccgagatc cccgcggctc cccaacctgc
     3061 gccggcgcca gtgagcccca ccgctccgcc cgcggaaatc ccggtgaaag ctcctccggc
     3121 gttcgcgtcg agccctgtgc cggccgttga cgcgccgcca tccagcgcga ccgcgggggc
     3181 ttcgaccgaa ccgaagccgc ccggggcttc cccgctcgtc gacatcgctg gctacatcca
     3241 gggcgagtat cagtctcatc aagactccga agatcagctg cggcaaggag ggagcttact
     3301 gaatcagaat cgctttctga ttcgccg
//
LOCUS       DASTKS010000972         6024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1942372, whole genome shotgun sequence.
ACCESSION   DASTKS010000972 DASTKS010000000
VERSION     DASTKS010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942372"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ35_20490"
     CDS             complement(<1..258)
                     /locus_tag="VFQ35_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933099.1"
                     /translation="MKPLEGLGTARKGAAATVTKATFWVPFCVALRYAYSLKTGEPTD
                     KIDNTIAALAGVAISAFLGSETVRWVWSLGEALLLRRLRLMS"
     gene            896..2509
                     /locus_tag="VFQ35_20495"
     CDS             896..2509
                     /locus_tag="VFQ35_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9933100.1"
                     /translation="MRGLRVGFLGALGSWFALLAPSCGGGSDNCVGRERCGCYANSTC
                     NDGLVCRSNVCVNDGSAGKGGNAGNGGSSVVAGGRNQAGGGGVAGDSLEEGGAGANSL
                     GGAMNEAGASALGGAATSLGGAVNEGGASGALGGGSSNGGGSSNGGTSSLAGSSSRGG
                     TSSLGGSSSVGGSSSRGGTSSLGGSSSLGGTGGNDCPSFKDEPEATVPTVFVLVDRSG
                     SMFNCLTSSSATTCPNLDDTAWTALKGPVEEMIADLQGSVRFGFGAFTGEQTGTCPMF
                     HSVAPKLDNAAAIRTLYDGLGAPTKGETPSSQVLTTVGTLLANDPSPGKKYILFVTDG
                     EPDFCDDGNPTCPVDAATWTLQSLSAQGIGTFVFGVDSKLSTVSGATLQAFANAGAGQ
                     PVATANASTIYPACHSNSLAWTQLFNSLQRTSGPLATYSASSGSAPVYRPNPTDQQAL
                     KDLFAGPVAGVKSCRFALAPGYHVVLDQLANAHVHIDGVELPRAGENGWNMTSTTVLE
                     LRGNSCVLWHDVKSRNITFDFPCGVVVAD"
     gene            3015..4781
                     /locus_tag="VFQ35_20500"
     CDS             3015..4781
                     /locus_tag="VFQ35_20500"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9933101.1"
                     /translation="MKRGFLARGALAVAVAACVACGGEDYPALPEVHESGGTNSGGES
                     SSSGGASSALGGGSSTAASTGFGGGSDVAFGGSGTAACSEAAPRRVRRLTLREVELTL
                     TDLLATPSGTFAWSARDPKVQGFDTNADALTVAGDGFEDFVDVAVLAVQGADVTVLAP
                     CVTSSDESCVPPFVADFAERAFGRPVTSVERQRLSDLYTSSRQNFDAGGALRLVLQAI
                     LSSPYFLYRSELGPSDAGTTTSLTPYEAANGLAFALTGARPDATLRARAKDDPDFMSL
                     GALREEARRLSGTDRAKQQLLRFLRQWLGMDDLRSVNKIPALFPEFTPEVKADLDREV
                     ETYLSQALAPGAGTLSALLSGSFAYPSQTVFDRLYAGDYRAKGKDVPVAGAFTPIAFE
                     LGLRKGVIGLPAWLAAHSPVHRSSPVDRGLAIRNRFFCQSLPSPPANALSVAPGPGDE
                     VATTRQKFEQHSNNDACRGCHQAMDPIGFGLEMMDAIGRYRDTENGLPVNSKGQLSGT
                     DVDGPFEGPAELADKLLASRIVRDCFTVQMFRYVAGRDTESGDSCLLQALQRRFAADE
                     LDISGLAQEIAVQTQLAPRSFE"
     gene            4778..>6024
                     /locus_tag="VFQ35_20505"
     CDS             4778..>6024
                     /locus_tag="VFQ35_20505"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9933102.1"
                     /translation="MSARVFGRRELLAAAGATLIVPAFLKRAFGAAQALPPRLVILMQ
                     ACGTHQQSFWPDANGASVALAPLLGTPLAQKTLVIKGIENRTQGLGNQHDRGFHSLWT
                     GVAPVGTPEDSFGGGPSIDQILKRRLAPSVPFPTLNVGVLAAYVAQKNGHRRSFSYLD
                     KAQQILTQVDPYRLYGKLFPSSSDASPETARRRLAAQQSVLDYAASDLSDLSLRLGSN
                     ERKKLEAHTTALREYENRLSATLSGGVAACAPPAALPVGLDVTLEDNVPVLADLMIDL
                     VALALSCNLTNIVTFQLGLCGNQWRYRWIGIDKDSHEEVAHADTPDESNHDAASYMTQ
                     ISTWAAQRVARLANALDGTLESDGTALDNSLVIWANENGTGFHNLDNLPMVFLGGARG
                     KLKQRGLLDPGTQSHYQLNTSVLR"
BASE COUNT         1040 a         1931 c         1961 g         1092 t
ORIGIN      
        1 gctcatgagc cgcagtcgac ggagcaggag ggcctcccca agactccaaa cccagcgaac
       61 ggtctcgctg ccgagaaagg cacttattgc aacgccggcg agcgcggcga tcgtgttgtc
      121 gatcttatcg gttggctcgc ccgtctttag cgaatatgcg tagcggagcg cgacgcagaa
      181 gggcacccag aacgtggcct tggtgaccgt tgccgccgcg cccttccggg cggtgcctag
      241 tccctccaat ggtttcattg cgctcagggc agtgcggctt gaatgaacat cgataagatg
      301 agcattgcgg tcatccacaa gggcgcagtg gggaagatgg ccagtagcaa gacgccgaga
      361 gccagaagtc gagccgctct gtagctcgtt cggccgcgtt gaggggtggg tacgccgtcc
      421 aaataacgct cgtgcgctag gcttgggtcg aagacagtaa agccacccgc tgaaccggta
      481 gggagcctgc cagagcgcct agcccaggct accgcgattg cgtcgggacc aaccctgcgt
      541 tcggactcgt ctaccgaatg cgcaactccg cgtttggaca tcctcgtatt ccttaccctc
      601 tatgttcgag agactcgcag tcgcgtccag caacaccgag atccccgtat tcgaccgcgc
      661 ctcagtcgcc gatagcgcag gcgcgctagg gacttccgcc cgcaaaagct gctcggcgcg
      721 atgcagttct acgccgacaa agcgccgctt cggccatgag ctcggagcaa atccagctcg
      781 catcgcaatc caggcgttgg accccgtcgt cgaatccaga actaaaccgc aaggtttcgc
      841 gcagcacccc aaaccgctct cgctgccgag ccaatctcgt gcatgatcgg gggtcatgcg
      901 tggcttgcgt gtgggtttct tgggtgctct gggtagctgg tttgctctct tggcgccgag
      961 ctgtggaggg ggctcggaca attgcgtcgg gcgtgagcgc tgcggctgtt acgcgaattc
     1021 gacgtgcaac gacgggctcg tgtgccgctc gaacgtttgc gtgaacgatg gcagcgccgg
     1081 caaggggggc aacgctggca acggcggaag ctcggtcgtg gcaggcggga ggaaccaagc
     1141 cggcggtggc ggcgttgctg gagactcgct cgaagaaggc ggcgccggcg cgaattcgct
     1201 cggcggagcg atgaacgaag ccggcgcgag tgcgctcggc ggggccgcaa catcactggg
     1261 cggggcagtg aacgaaggcg gagccagcgg ggctctcggc ggcgggtcat cgaacggcgg
     1321 cgggtcatcg aacggcggaa cctcgtcgct cgccggctct tcatcgaggg gcggcacgtc
     1381 gtcgctcggc ggctcttcat cggtgggtgg ctcatcatcg cgtggcggca catcgtcgct
     1441 cggcggctcg tcgtcgctcg gcggaactgg aggcaacgat tgcccgtcgt tcaaagacga
     1501 acccgaggca accgtgccca ccgtcttcgt tctcgtcgat cgctcgggaa gcatgttcaa
     1561 ctgcctcacc tcgtcctccg cgacgacgtg tccgaacctc gacgataccg cgtggacggc
     1621 cctcaagggc cccgtcgaag aaatgatcgc cgacctccag ggctccgtgc gcttcggctt
     1681 cggagctttc acgggcgagc agacgggcac ctgccccatg ttccacagcg tcgctccaaa
     1741 actcgacaac gccgcagcga tccgcacctt gtacgacggc ttgggggcac cgaccaaggg
     1801 tgaaactcca agcagccagg tcctcaccac cgtgggcacc ttgctcgcga acgacccgag
     1861 ccccggcaag aaatacattc tcttcgtcac cgacggcgag cccgactttt gcgacgacgg
     1921 caatcccaca tgccccgtcg acgcggcgac ttggaccctc cagagcctct ccgcgcaagg
     1981 catcggcacc ttcgtcttcg gtgtcgactc caagctcagc accgtttcgg gggcgacgct
     2041 ccaagccttt gccaacgctg gagccgggca acccgtcgcg actgcgaacg cctcgacgat
     2101 ctatccggct tgccactcga actcgctggc ttggacccaa cttttcaatt ccctgcagcg
     2161 cacctcgggt ccgctcgcca cctactctgc cagcagcggt agcgcgcccg tttatcgccc
     2221 gaacccgacc gatcagcagg cattgaaaga cctcttcgcg ggacctgtcg caggcgtgaa
     2281 gagctgccgc ttcgcgctcg cccccggtta tcacgtcgtg ctcgaccagc tcgccaacgc
     2341 gcacgtccac atcgacggcg tcgaactccc gcgcgccgga gaaaacggct ggaacatgac
     2401 gagcacgacc gtactcgagc tgcgaggaaa ctcctgcgtc ctctggcacg acgtaaagag
     2461 ccgcaacatc accttcgact tcccgtgcgg agtcgtggtc gccgactgag ccgagcgccg
     2521 acgggccctc cttcgatgct gagggcgcgc cgaccccacg ccacggcgcg agcgcgccag
     2581 cgcggaggat cgcccatgtc gacgttgagg cggtggagcg aatcgcaggg aaagtgaaac
     2641 ccgctcgttc ccgcgcgaca ctctgcggcg cggcgggtgg ttccgagccg cggcggcgcg
     2701 gaaaatctcc aatatcgaag ttggatgctt atgtgccccg tcgcggttca cggtgacgcc
     2761 tcgcggcgcg gggcgttcgc ggggttctgc ggacctggaa agacggttta ggcccggatc
     2821 ccgctagttt ggctcttttc gagtggatgg gcgtcgtttt cgagacggtt ttcccctggt
     2881 gcggacaatg gcactggggc ggtaccgcgt tggggctgct cgttacagaa ctcgaaacgc
     2941 gttagggctg ctcgttacag aagtcgaatg aggctttgcg ggctgctggt tggattggtg
     3001 gtatgctgcc ggtcgtgaaa cggggtttct tggcgcgtgg tgcgctcgcc gtcgcggttg
     3061 ccgcctgcgt cgcgtgcggc ggtgaagatt accctgcgct tcccgaggtt cacgagtcgg
     3121 gggggacgaa ttcaggtggc gaatcgtcgt cgtcgggtgg tgcgtcgtcg gccttggggg
     3181 gagggtcgtc gacggcggcg agcacgggtt ttggtggtgg gtccgatgtg gcgttcggcg
     3241 gtagtgggac cgcggcttgt tccgaggccg ctcctcggcg ggttcggcgt ctcacgttgc
     3301 gagaagtgga gctcacgctg accgacttgc tcgcgacgcc gtccggaact ttcgcgtggt
     3361 cggcgcgaga tccgaaagtg cagggttttg acaccaatgc cgacgcgctc acggtcgccg
     3421 gcgacggttt cgaagacttc gtggacgtgg cggtactagc cgtgcagggt gcggacgtga
     3481 ccgtgctcgc gccgtgcgtc acgtcgtcgg acgagtcgtg cgtgccgccg ttcgtggctg
     3541 actttgccga gcgcgccttc gggcggccgg ttacgtcagt ggagcggcag cgcttgagcg
     3601 acctgtacac gagcagtcga cagaacttcg acgcgggcgg tgcgttacga ctcgtgctgc
     3661 aagcgatttt gagctcgccc tactttttgt atcgtagcga gctcggccca agcgacgcag
     3721 gcacgaccac ctcgctcacg ccgtacgagg cggctaacgg gcttgcgttc gctctcaccg
     3781 gcgcgcgtcc ggacgctacc ttgcgtgccc gcgcgaagga cgaccccgac ttcatgtcgc
     3841 tcggagcact gcgcgaagag gcgaggcggt tgagcgggac cgaccgcgcc aaacagcagc
     3901 ttttgcggtt cttgcggcag tggctgggga tggacgatct tcgcagcgtc aataagatcc
     3961 cggcgctctt tcctgagttc acgcctgagg tgaaggccga tttggatcga gaggtggaga
     4021 cttacctttc gcaagcgctg gcgccgggcg cgggcacgtt gtcggcgttg ctctcgggct
     4081 cgttcgcgta ccccagccaa accgttttcg atcgtcttta cgcgggcgac taccgcgcga
     4141 aaggcaaaga cgttcccgtc gcgggcgcat tcacccccat cgctttcgag ctcggcttgc
     4201 gcaagggagt cataggccta cccgcttggc tcgcggctca ttcccccgtt caccgttcga
     4261 gcccggtcga tcgtgggctc gcgattcgca atcgcttctt ctgtcagtcg ctgccgagcc
     4321 cgccggcgaa cgcgctgtcg gtagccccgg ggccaggcga cgaagttgca accactcgac
     4381 agaagttcga acagcactcg aacaacgacg cgtgccgcgg ttgccaccag gccatggacc
     4441 caattgggtt tgggctcgaa atgatggacg cgatcgggcg ctaccgcgac accgagaacg
     4501 gcttgccggt gaactccaaa gggcaactca gcggtaccga tgtcgacggc ccgttcgaag
     4561 ggcccgccga gctcgcggac aagctgctcg cgagccgcat cgtgcgcgat tgcttcaccg
     4621 ttcagatgtt ccggtacgtc gccgggcgcg atacggagag cggagactcg tgtctccttc
     4681 aagctctgca gcgacgcttc gcggctgacg agctcgacat ttcgggtctt gctcaggaga
     4741 tcgccgtgca aactcaactt gcgccgagga gcttcgaatg agcgcgcgcg tgttcgggcg
     4801 gagggaacta ctggcggcgg cgggggcaac gctgatcgtg ccggccttct tgaagcgagc
     4861 ctttggcgcg gcccaggcgc ttccgccgcg gctcgtgatt ctcatgcaag cttgcggaac
     4921 ccaccagcag agcttctggc cagacgcgaa tggcgcgagc gttgcactcg cgccgctgct
     4981 cggcactccg ctcgcgcaaa agacgctcgt catcaaggga atcgagaacc gcacccaagg
     5041 ccttggtaac cagcacgatc gtgggtttca cagtctgtgg accggtgtgg ctcccgtcgg
     5101 cacccccgag gattcgttcg gcggtggccc ttccatcgac caaattctga agcgcaggct
     5161 cgcgccgtcc gttccattcc cgaccctcaa cgttggggtg ctggccgcgt acgtggctca
     5221 aaagaatggg catcggcgct cgttcagcta cctcgacaag gcgcagcaaa ttctgactca
     5281 ggtcgacccg taccgtctct acggcaagct ctttccgagc agcagcgacg cgtcgcccga
     5341 aacggctcgg cgcaggctcg ctgcgcagca aagcgtcctc gactatgcgg cctccgacct
     5401 gagcgatcta agccttcggc tcggttcgaa cgagcgaaag aagctcgaag ctcacacgac
     5461 cgcgctgcgc gagtacgaaa atcggctcag cgcgacgctg tcgggcggcg tagcggcctg
     5521 cgcgccgccg gcagctttgc ccgtggggct cgacgtcacg ttggaagaca acgtgcccgt
     5581 gctcgccgac ttgatgatcg acttggtcgc gctcgctctg tcgtgcaacc tcaccaacat
     5641 cgtcacgttc cagctcggct tgtgcggcaa tcagtggcgc taccgctgga tcgggatcga
     5701 caaagactca cacgaagagg tcgcgcacgc ggatactccc gacgagagca accacgatgc
     5761 cgcgagctac atgactcaga tcagcacttg ggccgcgcag cgcgtcgcac gcctcgccaa
     5821 cgccctcgac gggacgctgg aatccgacgg caccgcgctc gataactcgc tcgtaatctg
     5881 ggcgaatgaa aacggcactg gcttccacaa cctcgacaat ttgccgatgg tcttcctcgg
     5941 cggggcacgc ggcaagttga agcagcgcgg cctcctcgat cccggaacgc agagccacta
     6001 ccagctgaat accagcgtgc tgcg
//
LOCUS       DASTKS010000973         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1271960, whole genome shotgun sequence.
ACCESSION   DASTKS010000973 DASTKS010000000
VERSION     DASTKS010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271960"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFQ35_20510"
     CDS             complement(<1..1203)
                     /locus_tag="VFQ35_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933103.1"
                     /translation="MRAALVSLVLLCACAATPPPPAAPPPPPPAPPPVAAPEPKPAPV
                     PPAPAACAPYLDAPVACDSGHSLAERLADALGENESLTRDRKLACVELDGGPAAGMLR
                     ALRADLAPSACADALVTPYLEAAQRPSPEIETVLVGLMLAGKLSRLAADPPRLEPPYD
                     KAKFLEFFEKTLKPWVIAEALSIGELSTQGAKLSGYGKAVAAIEAGISDLRFVQMARN
                     IPLPTEFAADRDARDAYYASLDQALEPRKARGRDAALVGLRLFAELGVLKDARLSRAR
                     ALLAELYGGSRVTALEGLALPELPPLRTDSVDQKLAARLPTYYALAFLSELKEVSDAK
                     LLRAFAERGLPTTFRTRLDGEKLSNDARFMYALLETQRGITYFSAPAFSHAAQVAAAA
                     VNDPGSELL"
     gene            complement(1289..2500)
                     /locus_tag="VFQ35_20515"
     CDS             complement(1289..2500)
                     /locus_tag="VFQ35_20515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9933104.1"
                     /translation="MSPLALLFVALFNSILGLSLLFPILGPLGRELGLSELQVGALTA
                     AYAVAQFAGSPYWGRKSEVLGRKPVLLRGIVGFGVTFLVFALLIACGQRGMLSPHALF
                     VSLLATRLLGGLYSSATLPTAQAYVADVTGRADRTAGMTLIGASFGLALIFGPVISGL
                     LARFGLLVPVYASATVALLNAVFVWLKLPEPASHIPTNRPPDLLPVASKVWTVLGVGF
                     ATTLASVCMEQTLAFYFQDRLGLSHRGAAQTFSWSLGVYGVVAVIVQGFFVRRVKWPP
                     LVLLRAGVPIAATGLLALIFAKGTVTLVIAMALQGLGQALALPGVTAALSLSVDDGEQ
                     GAVAGLNGASQSLGRMLGPLLGTGLYELRPEYPYGFGALILCVVFAFLVLSRSPHLRA
                     MKLEESARAES"
     gene            2654..3070
                     /locus_tag="VFQ35_20520"
     CDS             2654..3070
                     /locus_tag="VFQ35_20520"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9933105.1"
                     /translation="MRHDDIIALLLRHGIQPSAQRVAVADYVLFTDEHPSADRVFNTV
                     RKTLPVLSRATVYNTLNLFVKQGLLRELVLAEGNVVFDPKLERHHHFVDETSGRIYDV
                     PWEALEVKKVAALEDYDVDEYQVVLRGRRSSSRRPK"
     gene            3150..3704
                     /locus_tag="VFQ35_20525"
     CDS             3150..3704
                     /locus_tag="VFQ35_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9933106.1"
                     /translation="MANIEELRQKIIDPLRDLRLNLESSTGPLAGSLDASQRWGVIVA
                     AACASRHAELSNALITAARTEVNQQVIDDGIAAAGLMAMNNVYYRFRHLIQKPSYSDM
                     PARLRMTRIAKPATNKADFELFCLAVSAINGCETCLRSHEEAVLSGGLSEAHVHDAVR
                     LAAAIHAAAVSLEISELDSVATPA"
     gene            3750..4232
                     /gene="dps"
                     /locus_tag="VFQ35_20530"
                     /gene_synonym="pexB"
     CDS             3750..4232
                     /gene="dps"
                     /locus_tag="VFQ35_20530"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HET9933107.1"
                     /translation="MKLYKSPSHLPEEARAKIVEVLNARLADGLDLHSQIKVAHWNIK
                     GPHFSSLHPLFETFAVSLANFNDAIAERAVTLGGKAFGTTRYVAGASRLPEYPQETTR
                     DLEHVRLLAERIDVYLVGLRESRGVSEKAGDTDTVDLFTGVITEFEKNAWFLRASLDS
                     "
     gene            complement(4374..4793)
                     /locus_tag="VFQ35_20535"
     CDS             complement(4374..4793)
                     /locus_tag="VFQ35_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933108.1"
                     /translation="MQDITTEPPGPPGSGAMGGSNAQVAGAPSITAGTASFGKGGATF
                     GSGSAAAGGALCPSAAFFPFLAGGAAGAAGAGAVNHPRNGFGCPATAPVVDTRCDWAQ
                     VQCVYDDFIDCEKTPNVIIFTCCNTKWDADLLCGPTR"
     gene            complement(4898..>5224)
                     /locus_tag="VFQ35_20540"
     CDS             complement(4898..>5224)
                     /locus_tag="VFQ35_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933109.1"
                     /translation="GEKAAPFLSQWLDDGSLQIFGGPLFKDRALHRVQRVDDRLGQTL
                     PPAVEGFSTRRVNFTFRYVPEQHVIPFDRLGQRAQQDVRGYVRELATHSAFFREALAR
                     ADGARP"
BASE COUNT         1001 a         1675 c         1702 g          846 t
ORIGIN      
        1 gaggagctca ctccccggat cgttcactgc ggccgccgcg acttgcgccg catgagagaa
       61 ggcgggcgcc gagaagtaag tgatgccgcg ctgagtctcg agcagcgcgt acatgaaccg
      121 agcgtcgttg ctcaatttct cgccgtcgag ccgagtacgg aacgtcgtcg gcagcccgcg
      181 ctccgcgaac gctctcagga gcttcgcgtc cgaaacttct ttgagctcgg acaagaacgc
      241 gagggcgtaa taggtgggga gtctggcggc gagcttttgg tcgacgctat cggttcgaag
      301 cggggggagc tccggcaggg cgagcccttc tagcgccgtc acgcggctgc cgccgtacaa
      361 ctccgcgagc agagcccgcg cccgcgaaag ccgcgcgtcc ttcaaaacac cgagctcggc
      421 gaacaagcgc agtcccacga gcgcggcgtc tcgaccgcga gccttacgcg gttcgagcgc
      481 ttggtcgagc gaagcgtagt aagcgtctcg agcatctcgg tcagcggcga actcggtcgg
      541 caacgggatg tttcgtgcca tctgcacgaa gcgaagatcc gaaattccgg cttcgatcgc
      601 cgcgaccgct ttgccgtatc cgctgagctt cgcgccctgc gtcgagagtt caccgatgga
      661 gagcgcctcc gcgatcaccc acggcttgag cgttttttcg aagaactcga ggaacttggc
      721 cttgtcgtac ggcggctcga ggcggggcgg gtccgcggcc agccggctca gctttcccgc
      781 gagcatcaaa ccgaccaaga ccgtctcgat ctcggggctc gggcgctgcg cagcttcgag
      841 atacggcgtg acgagcgcat cggcgcaggc agagggcgcc aggtctgccc ggagcgcgcg
      901 caacatcccc gcggctggcc caccgtcgag ctcgacacat gcaagcttgc gatcgcgcgt
      961 cagcgactcg ttctctccga gcgcatccgc caggcgctcc gcgagcgaat gcccgctgtc
     1021 gcacgcaact ggcgcgtcga ggtaaggcgc gcaagccgct ggtgcggggg gcaccggcgc
     1081 cggcttgggc tccggagccg cgactggcgg tggtgccggc ggaggtggtg gcggagccgc
     1141 gggaggcgga ggtgtcgcgg cgcaagcgca gagcaaaacc agggagacga gggcggctct
     1201 catcgggtgc atcgtaggcc ggtgcggacc cgaggaggaa cccgcatcga ctcctcgaca
     1261 cgccttttcg ccgaaggttc ccggaaattt acgattcggc gcgcgccgat tcctcgagct
     1321 tcattgcgcg caggtgaggc gaacgcgaca aaaccaggaa cgcaaaaacg acgcacagga
     1381 tgagcgcccc gaagccgtac ggatactcgg gccggagttc gtacaacccg gtgccgagca
     1441 acgggcccag catgcgcccg agtgactgcg acgcgccgtt caatccggcg accgcgcctt
     1501 gctcgccgtc gtccacgctg agcgagagcg ctgcggtgac cccgggcaga gcgagtgctt
     1561 ggccgagccc ctgcagcgcc atggcgatga cgagcgtgac cgtgcccttc gcgaagatca
     1621 gcgccagcaa accggtcgcc gcgatcggca ccccggcgcg caacagcacg agcggcggcc
     1681 actttacgcg acgcacgaag aagccctgca cgatcacggc gaccacgccg tagactccga
     1741 gcgaccagct gaaagtctgc gcggcgcccc ggtgcgacag ccccaacctg tcttgaaagt
     1801 agaaggcgag agtctgctcc atgcagacgg acgcgagcgt cgtcgcaaag cccaccccga
     1861 gcacagtcca taccttggac gcgaccggca agagatctgg cggccgattc gtcggtatgt
     1921 gggaggcggg ctcgggcaac ttcaaccaca cgaatacggc gttcagcagc gcgaccgtcg
     1981 cggaagcgta gaccggtacc aacaagccga accgcgcgag aagccccgag atcaccggtc
     2041 cgaagatcaa tgcgagccca aacgatgcgc cgatcagcgt catgccggcc gtgcgatcgg
     2101 cgcgaccggt gacgtcggcg acgtacgctt gagcggtggg tagtgtggcg gacgaataca
     2161 agccgccgag cagcctcgtc gcgagcaagc tgacgaagag ggcgtgcgga gagagcatgc
     2221 cccgctgccc gcacgcgatg agcaacgcga agaccaggaa tgtcacgccg aagccgacga
     2281 tgccgcgcag cagcaccggc ttgcggccga gcacctcgct cttgcgaccc cagtatgggc
     2341 ttccggcgaa ctgcgccacg gcgtacgccg cggtgagcgc gccgacctgc aattcggaca
     2401 aaccgagctc tcgtccgaga ggacccaaga tcggaaacag caggctcaag ccgagaatgc
     2461 tgttgaagag cgcgacgaac aggagggcaa gggggctcat ggcggcggag ggcggaggcc
     2521 gccacacggg cgactctgtg aacgcgcggg aacgtagcac tctgcgcgtt gcggcgatgg
     2581 tttcgctcga tcacccgccg cgagccgagg aatcttgctt gacgtggtca cgttttagac
     2641 gcattctaat gtcatgcgtc acgacgacat catcgcgcta cttctgcgtc atggcattca
     2701 gccatccgct cagcgcgtgg ccgtcgcaga ctacgttctt ttcaccgacg agcacccgtc
     2761 ggccgaccgt gtgttcaaca cggttcgcaa gacgctaccg gtgctttcgc gagccaccgt
     2821 ctacaacacg ctgaacctct tcgtaaagca ggggctcctg cgcgagctcg ttctcgcgga
     2881 gggaaacgtg gtgttcgacc caaagctcga gcggcaccat cacttcgtcg acgaaacgag
     2941 cggtcgcatc tatgacgttc cgtgggaggc gctcgaagtg aagaaggtcg ccgccctcga
     3001 ggactacgac gtcgacgagt accaagtcgt gctgcgtggc cgtcgtagta gctcgcgccg
     3061 tccgaagtga gatctctcaa tcgcgtcggc gattttcctt tgtttgttgg atagacagtg
     3121 tctaaacttg gatgaaatag aggagaccaa tggcgaacat cgaagaactc cgacagaaga
     3181 tcatcgaccc gctgcgtgac ctgagactca acctggagtc gtcgaccggc cccctcgcgg
     3241 gtagcctcga cgcttcgcag cgttggggag tgattgtcgc ggcagcttgc gcatccaggc
     3301 acgcggagct gtcgaacgct ttgatcaccg cggcccgcac cgaagtgaat cagcaggtga
     3361 tcgacgacgg catcgccgcc gccggcttga tggcgatgaa caacgtgtac taccgctttc
     3421 gccacctcat tcagaagcct tcgtacagtg acatgccggc gcgcctgcgc atgacgcgca
     3481 tcgcgaagcc tgccaccaac aaggccgact tcgagttgtt ctgtctggcc gtgagtgcga
     3541 tcaatggctg cgagacgtgc ctgcgatccc acgaggaagc cgttttatcg ggcggactca
     3601 gcgaggccca cgtccacgac gcggttcgtc tcgcggcagc cattcacgcc gcggccgtgt
     3661 ctctcgagat ctccgagttg gactccgtcg ccaccccggc gtgagactct gcacgcactc
     3721 ctcgctcgaa cgatcgaaag gaagccgaca tgaaactcta caagagcccg agtcacttgc
     3781 ccgaagaggc ccgcgcgaag atcgtggaag tcttgaatgc gcgcctcgcc gatgggttgg
     3841 accttcattc tcaaatcaag gtcgcgcact ggaacatcaa gggccctcat ttcagctcgc
     3901 tgcatccgtt gttcgagacg ttcgcggtga gcctcgcgaa tttcaacgat gccatcgccg
     3961 agcgcgccgt gaccctgggc ggcaaagcgt tcggcaccac gcgctacgtc gctggcgcgt
     4021 cgcgactgcc ggagtatccg caagaaacca cccgtgacct cgaacacgtg cgccttctgg
     4081 cggagcgaat cgacgtctac ctcgtcggcc tgcgcgagtc gcggggtgtc tccgaaaagg
     4141 ccggtgacac ggatactgtc gacctcttca cgggcgtgat taccgagttc gagaagaacg
     4201 cctggttcct ccgcgcgtca ctcgatagtt aatcggcgcg gtcgttcaca ctgagcgcgg
     4261 tgagtcgcgc cgcgctcagg gccgggcgaa tcgggctcga caggaaagag tggtttccac
     4321 ccgtgctccg aagaaagggc gggcacgcgg tatgacttcc cattcgcggg tccttagcgc
     4381 gtgggaccgc acaggagatc ggcgtcccac ttcgtgttgc agcacgtgaa gatgatgacg
     4441 ttcggcgtct tttcacagtc gatgaagtca tcgtagacgc attgaacttg tgcccagtcg
     4501 caccgagtat cgacgacggg agcagtggcc ggacacccga acccgtttct tgggtggttc
     4561 accgcgcccg cacctgcggc gccggcggct ccacccgcga ggaacgggaa gaacgccgcg
     4621 gacgggcaca aggcaccacc cgcagccgcg cttccgcttc cgaaggttgc gcctcccttg
     4681 ccaaagctcg cggtcccggc cgtaatcgag ggtgccccag caacctgtgc gttggagccg
     4741 cccatcgcgc cgcttccggg tggtccaggt ggctccgtcg ttatgtcctg caccccctcg
     4801 caggaggggc agagccaaaa aaagcagagt ccgatcccgc agagtttttg agttgcccgc
     4861 cgcaccggcg aagcttagcg ccgggctccg cgacacttca aggtcgcgcg ccgtctgccc
     4921 gcgctagtgc ttcgcggaaa aacgcggagt gggtggcaag ctcgcggaca tagccgcgca
     4981 cgtcttgctg cgcgcgctgg ccgaggcggt cgaaggggat cacgtgctgc tcgggaacgt
     5041 agcggaaagt gaagttgacg cggcgagtcg agaaaccttc gaccgcgggg gggagagtct
     5101 gacccagtcg atcgtcgacg cgctgaacgc ggtgaagcgc gcggtctttg aacaggggtc
     5161 ccccgaagat ctgcaacgaa ccgtcgtcga gccattgcga caagaacggc gcggcttttt
     5221 cgcc
//
LOCUS       DASTKS010000974         4728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1942738, whole genome shotgun sequence.
ACCESSION   DASTKS010000974 DASTKS010000000
VERSION     DASTKS010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942738"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VFQ35_20545"
     CDS             <1..1286
                     /locus_tag="VFQ35_20545"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9933110.1"
                     /translation="GRRVPPTRDSRRGERRVMSLAAPAARLAATSAKIALFAVGFALA
                     CSRAPDEGKARVIPSSRPSSSSNSTPPAPSVAKCPPGTALVPGGEFWVGAEKGEHYSD
                     DESPRFLTRVMSFCADLTEVTVAAYGRCVEAGQCTPPRAKRTLCNGSHPERADHPINC
                     VTFGQAEAYCKSTSARLPTEVEWEYMARGGSSYLKYPWGSDSPDGRACWKMPRTCPVR
                     SFPAGAFGLFDVSGNVWEWTDSWYGAYPWPPTQGFAKVYRGGSFSRRFEKWMHTRLRN
                     RADPDESGSHLGFRCVATLAGETCPFGQAPDGSCLHGVIARDCPPGLTFNGVRCAEPG
                     APRCREGRVEKAGYGCVLAEEQPPETRDIEAESRGVSRSRATEFDADCAKNQPSRPRA
                     FRYVGGTHDGRNLVSRRAGCKNRDVGVGWNSVCCP"
     gene            1491..1838
                     /locus_tag="VFQ35_20550"
     CDS             1491..1838
                     /locus_tag="VFQ35_20550"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9933111.1"
                     /translation="MRDDVGTESESDRVSLARQALATGRWVLVDYFERDGQIHILARR
                     DTTFEALSVLTASERAVVELAARGLHNKAIAFELGLAHSTVRVLSARAASKLGARSRR
                     ELVDLVTWTKERG"
     gene            complement(1857..3185)
                     /locus_tag="VFQ35_20555"
     CDS             complement(1857..3185)
                     /locus_tag="VFQ35_20555"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9933112.1"
                     /translation="MRFVEMAGFCVTLGLSATIAVGCSVGARDEENVAGQALELKRHN
                     PCPEEARLFVPDPNPAATEQIRSLRRAHDRKNAALVEAMVDTPQSVWLVGGTPDDVAD
                     RVKTTMKKARSQRAIPVFIAYNIPFRDCSGLSAGGAQNTAEYLAWIDAIARAIGRADA
                     MVILEPDSLGIIPYHVALGGSEEWCRPADADATTASAERYAQLNSAVDRLKQQPNVRV
                     YLDGTHSHWLGSGDAAWRLSRAGIERADGFFLNTSNYQASERLEKYGTWVSSCLAFAN
                     NPDEGGWRVGRYDWCGSQYYPANVDDFSTWGATDDWYAANLGSAIPSTHFVIDTSRNG
                     QGPWVPPSDAPAGDPQDWCNPPGRGLGYRPALCSTEDGASTTSCSRTPLLEANLWLKI
                     PGESDGQCTRWAPGGTTDPARGMEDPQAGAWFPEQVLELVQLASPPLARH"
     gene            complement(3348..3857)
                     /locus_tag="VFQ35_20560"
     CDS             complement(3348..3857)
                     /locus_tag="VFQ35_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9933113.1"
                     /translation="MLHIGDSFAESLGAPLGRHLQAAGVRSVLESKTPSYIPTWAGNG
                     ELRRLLEKHKPDLVLITLGGNEFEIPTPNDRVDPVKRLVRLVGDRPCVWITPPRWKQD
                     SGVLAIIKENSAPCRYLDSDALVHDLPLARDKVHPNEAGRELWAQAVLAWLKAERAPS
                     DGHPWSLKR"
     gene            4249..>4728
                     /locus_tag="VFQ35_20565"
     CDS             4249..>4728
                     /locus_tag="VFQ35_20565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933114.1"
                     /translation="MRLLEPGDLIEGKYRINRQIGEGGMGAVFEGEALRIHRRIAVKV
                     LFPHAGAEDDLARRFQLEAQAAGRIGSSHIVEVIDLGTLPSGSHYMVMELLDGESLRS
                     RIQSRGRLRPEEIYPIAMQLLEGLGAAHRAGILHRDLKPDNVFLVRQAQGDFVKLLDF
                     "
BASE COUNT          810 a         1483 c         1587 g          848 t
ORIGIN      
        1 gagggcggcg tgtcccccca acccgagatt cgcgacgagg cgagcgccgc gtgatgtcgc
       61 tcgctgcccc cgcggctcgg ctcgctgcga cttccgcgaa gatcgcgctc tttgcggtcg
      121 gctttgcgct cgcttgctcg cgagccccgg acgaggggaa ggcgcgtgtc atcccatcgt
      181 cacggccgag cagctcatcg aattcgacgc ctcccgcgcc gagtgtggcg aagtgccctc
      241 ccggtacggc gctggtcccc ggcggtgagt tctgggtcgg cgcggagaag ggagaacact
      301 atagcgacga cgagagcccg cgatttttga ctcgagtcat gagcttctgt gcggatctga
      361 ccgaggtcac ggtagcggcg tacggccgat gcgtagaggc tggtcagtgc acgccgccgc
      421 gggcgaagcg tacgctgtgt aacggctccc atccggagcg cgccgaccac ccaatcaact
      481 gcgttacttt cggtcaggca gaggcctatt gcaaatccac gagtgcgcgc ctgccgaccg
      541 aggtggagtg ggagtacatg gcacggggcg gctcgtcgta cctgaagtat ccgtggggca
      601 gtgactcccc tgacggtcgg gcatgctgga agatgccgcg gacatgtccg gtcagaagct
      661 tcccggcggg tgcgtttggt ctcttcgacg tgagcggcaa cgtctgggaa tggaccgaca
      721 gctggtacgg ggcctacccc tggccaccga cgcaggggtt cgccaaggtc tatcggggag
      781 gaagcttcag ccgccgcttc gaaaaatgga tgcatacgcg cctcagaaat cgcgccgatc
      841 cggacgaatc gggttcccac ttggggtttc gctgcgtcgc gacgctcgca ggtgaaacgt
      901 gcccgtttgg ccaggcgccc gatggtagct gcttgcacgg cgtgatcgcg cgtgactgtc
      961 ccccggggct cacattcaac ggtgttcgct gcgccgagcc gggagctccg cgttgccggg
     1021 aaggtcgcgt cgagaaggct ggttacggct gtgtcttggc cgaggagcaa ccgccggaaa
     1081 cgcgagacat cgaggcagag agtcgcgggg tgagccgctc gcgagccact gagttcgacg
     1141 cagactgcgc gaagaaccaa ccctcgcgcc cgcgcgcgtt tcgctacgta ggtggaaccc
     1201 acgacgggcg taatctcgtc agtcggcgcg ctggctgcaa gaatcgtgac gtgggagtcg
     1261 gctggaactc cgtctgctgc ccctgagtga ttgcttcgtt cgttacaaca aatttgaggc
     1321 aaacacttcg cgccgcattc gcgtgcgaaa agacgcgctg acggccaaaa gccagaagcg
     1381 gcgacgactc tgccgaaatt cgagcagaag aagtcactat tgtcgcccgc tgcgacatat
     1441 tgagagtgtt gccgagcagc gcgaatccgt ggctgcggag ggagtgcacg atgcgcgacg
     1501 atgtcggtac cgagtccgaa agcgaccgcg tctcgctcgc gcgccaagcg ctcgccacgg
     1561 ggcgctgggt gctcgtcgat tacttcgagc gcgatggcca aattcatatt ctcgcgcgga
     1621 gagacacgac gttcgaagcg ctctcggtgc tcaccgcgag tgaacgcgct gtggtcgagc
     1681 tcgccgcccg cggccttcac aacaaggcga ttgcgttcga gctcgggctc gcgcactcga
     1741 cggtgcgggt gctctctgcg cgagcggcct ccaagctcgg agctcgttcg cgcagagaac
     1801 tggtcgacct cgtcacgtgg accaaggagc gcggctgaag ccgcttcgcg agagatctag
     1861 tgccgggcga ggggagggct cgcgagctga actagctcga gaacttgctc gggaaaccac
     1921 gcgcctgctt gcgggtcttc catgccgcgc gccgggtcgg tggtgccgcc gggggcccag
     1981 cgggtgcact ggccgtcgga ctcccccggg attttgagcc agaggttcgc ttcgagcagc
     2041 ggtgtgcgcg agcaggacgt ggtcgatgcc ccgtcttcgg tggaacacag cgccggccgg
     2101 taaccgagcc cgcgcccggg agggttgcac caatcctgcg ggtcgcccgc cggcgcatcg
     2161 ctgggcggca cccacggccc ttgtccgttg cgcgaagtat cgatcacgaa atgtgtgctc
     2221 gggatcgcac tgccgaggtt tgccgcgtac caatcgtcgg ttgctcccca cgtcgaaaag
     2281 tcgtcgacgt ttgcaggata gtactggctg ccgcaccagt cgtagcgtcc cacgcgccag
     2341 cccccttcat ccgggttgtt cgcgaacgcg aggcaggagg agacccacgt gccgtatttt
     2401 tcgagcctct cgctcgcctg gtagttcgac gtgttcagga agaagccatc ggcgcgctcg
     2461 atgccggcgc gcgacagtcg ccaagccgcg tcgccgctcc cgagccagtg actgtgagtc
     2521 ccatccaaat agactcgcac gttgggctgc tgcttcaggc gatcgacggc agagttcagc
     2581 tgcgcgtagc gctccgcgga cgcggtggtt gcatcggcgt ccgccgggcg acaccactct
     2641 tcggaacctc cgagcgcgac gtgatacggg atgatgccga ggctatccgg ttcgaggatc
     2701 accatcgcgt cggccctgcc aatcgcgcgg gcgatcgcgt ctatccacgc cagatactcg
     2761 gccgtgttct gggccccgcc ggcggagagc ccgctgcagt cgcggaaagg aatgttgtaa
     2821 gcgatgaaca cggggattgc gcgctgcgaa cgcgccttct tcatcgtcgt cttcacccga
     2881 tcggcgacgt cgtccggcgt tcctcccacc aaccaaaccg actgcggggt gtcgaccatg
     2941 gcctccacga gcgcggcgtt ctttcgatcg tgcgcgcgcc gaaggctgcg aatctgttcg
     3001 gtcgctgcgg ggttcgggtc gggaacgaac aaccgcgctt cctccgggca gggattgtgc
     3061 ctcttgagct cgagagcctg acctgcgacg ttctcttcgt cgcgcgcccc gacgctgcag
     3121 ccaaccgcga ttgtcgccga caaccctagg gttacgcaaa agcctgccat ttcgacgaaa
     3181 cgcatcgtga acctcccttt gcgagccgcc cggcacgctt cggcgccgaa cacagctccc
     3241 accgcaatca gtatgcgtcg cgcgtcgagc gagcaagaac tctcgagttt acggtcggcg
     3301 taaaccgacg cgcatcgtcg actcaactcg agtctttgtg gccgcgatca gcgcttcaat
     3361 gaccacggat gtccgtcgct cggcgcccgc tctgctttca accaagcgag cacggcctgc
     3421 gcccaaagct cacgtcccgc ttcgttcggg tgaaccttgt cgcgcgcaag tggcaaatcg
     3481 tgaaccagcg cgtcgctgtc caaataccgg cacggcgcgc tgttctcttt gatgatcgcg
     3541 agcacgccgg aatcctgctt ccagcgcggc ggggtgatcc acacacaggg gcgatcgccg
     3601 accagccgca ccagccgttt caccgggtcg acgcgatcgt tcggagttgg aatctcgaac
     3661 tcgttgccgc ccagcgtgat gagcaccaga tccggtttgt gtttctcgag cagtcgccgg
     3721 agctcgccgt tgcctgccca ggtcgggatg taactcggtg tcttcgattc gagcacgctg
     3781 cgcacgccgg cagcttgcaa gtgccttccg agcggtgcgc cgagcgactc ggcgaaggaa
     3841 tcaccgatgt ggagcacggt cgttcccgac ggaatttcct gtgtcgtggc gggcggcgcg
     3901 gagggccgct ccgtctctcg cggggacgag ccagtggaaa ccggttccgt agaggctgca
     3961 attgctccgg atggcgcgga tacgctgggc gctggcggcg gaggtggcga ggccgaagcg
     4021 ggagctggcg gcgggctttg gcagcccaaa agcatcgcta tcagaccgac ggctgaaagg
     4081 tgggaacgac tcggagcgtt gaatgaaagc atgcgggcca cgcggggaaa cgatagttcg
     4141 aacccttacc agaactcgcg gtcggtcgaa ggcgcacccc tcgccgaggg ggatggtttt
     4201 gcaccgaaat cgccctccga gccgggcaga atgctaagct ccgggggctt gcgacttctg
     4261 gaacctggcg acctaatcga gggtaagtac cggatcaacc gtcagatcgg cgaaggtgga
     4321 atgggggcgg tcttcgaggg ggaagcgctc cggatccatc gccgaatcgc ggtgaaggtg
     4381 ctgtttccgc acgcgggtgc agaagacgat ctcgcgcgtc gatttcagct cgaagcgcag
     4441 gctgccggtc gcattggctc gagtcacatc gtcgaggtga tcgatctcgg gacgctgccg
     4501 agcggctctc actacatggt gatggagctg ctcgatggcg agagcctgag gtcgcgcatt
     4561 cagagccgcg gccggctgcg cccggaggag atctatccga tcgcgatgca gctgctcgag
     4621 ggtcttggcg cagcgcaccg tgcggggatt cttcaccgcg acctcaagcc ggacaacgtc
     4681 ttcttggtgc gccaggccca aggtgacttc gtgaagctgc tcgacttc
//
LOCUS       DASTKS010000975         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_824398, whole genome shotgun sequence.
ACCESSION   DASTKS010000975 DASTKS010000000
VERSION     DASTKS010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824398"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VFQ35_20570"
     CDS             complement(<1..1186)
                     /locus_tag="VFQ35_20570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933115.1"
                     /translation="MDDSPLEAEFAKTILEQQHDVQLFFDGATVIERVTQGILPDVLI
                     LDWHMPNVSGLEVCRFLRQSHDEASLPILILTATAGRDDLLEGLAAGANDFVTKEFDS
                     AELVARVATLVRSRRLHDRAKQSELSARRALAVAEQASRAKDVFLATVSHELRNPLNA
                     LLGWVTLVARPSVDPALLRRGLEIIERNAKLQVQLIEDILDISRVVSGKLHLETRTVD
                     VARIVQAVVESALPNAESRGLTVQTKLEPSAATVGDPDRLHQAFYNVLSNAMKFSRKG
                     GKIEVSVRRVAEHVEVRIQDEGAGISPEFLPFVFDRFRQQDSTASRNHSGLGLGLALV
                     KSIIEAHGGRVRVTSLGKNRGTTVVLDVPECDVRSSRQPSTETPSSPSAARRSHGQAL
                     VGV"
     gene            1398..1784
                     /locus_tag="VFQ35_20575"
     CDS             1398..1784
                     /locus_tag="VFQ35_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933116.1"
                     /translation="MASILVVDDSQVMRDMVVACLRAISETSFTHAASGLEAIERLSL
                     KKFDLMVLDLNMPDIGGIEVLEFVRGQDALRRLPVLIVTTRGDQDSRQRVLSAGASAF
                     LAKPFTPQAIISEVNALLSARAEPSA"
     gene            1781..>3166
                     /locus_tag="VFQ35_20580"
     CDS             1781..>3166
                     /locus_tag="VFQ35_20580"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9933117.1"
                     /translation="MTVETNLKEFFTAYLVEMGEYLVAANTQLLALEASLRAGGANPR
                     AVRETFRALHTMKGLSAMVGVEPIVGVAHRLEEFLRDYDKRGTKPPLASIDVLLRGVR
                     TIELCIEELGSGSEPHPIPDNLFRDLEAIEPSGQSLEAPPPALSLAPDLVRKLAPFEL
                     AQLTQAEQSGQRALRADFCPSPERAAAGLSIATVRERVAKVAEIVKILPLSVPVSPDA
                     PGGLAFALLLVTSESDAAIADAIGVPATTLSALCTPSADAGAALDVPLPAEDRQEPVR
                     DGGAPQRSSLRVDVARLDDVMERLSALIVTRFRMARAVATLEAAGANTAEVTAILGET
                     ARQLRDLRAAVVGVRMVPVADLLERVPLLVRGLRRQTNRQVRLELVNGNAELDKSVSE
                     RLFPAIVHLVRNAVDHAIETPDERLRRGKPQEGLLRIVCASRSNNRLDVSVEDDGRGI
                     DAEAVARRAGRP"
BASE COUNT          541 a         1014 c         1030 g          581 t
ORIGIN      
        1 ccacgccgac gagggcttgc ccgtgcgatc gacgagcagc agagggactg gacggggttt
       61 cggtcgaagg ctgtcgcgag cttcggacat cgcactcggg cacgtcgagc actaccgtgg
      121 tgccgcggtt cttgcccagg ctcgtcacac gcacgcggcc accatgagcc tcgatgatgc
      181 tcttcacgag cgcgaggccc aacccgagcc cggagtgatt gcgcgacgcg gtcgagtctt
      241 gctgccggaa ccggtcgaac acgaacggta agaactcggg agaaatccca gcgccctcgt
      301 cctgaatccg aacctcgacg tgctcggcga cgcgcctgac gctgacctcg atttttccgc
      361 ccttacggct gaacttcatg gcgttgctca agacgttgta aaacgcttgg tgcaatcggt
      421 ccgggtcgcc aaccgtggcc gcagacggct cgagcttcgt ttgcaccgtc aagccgcggc
      481 tttctgcgtt gggtagagcc gattcgacga cagcctgcac gatgcgcgcg acgtccaccg
      541 tgcgagtctc gaggtggagc ttgcccgaaa ccacacgaga gatatcgagg atgtcttcga
      601 tcaactgaac ttggagcttc gcgttgcgct ctatgatctc gagccctcgg cgtaggagcg
      661 cagggtccac actcggccgc gcgacgagcg tgacccagcc gagcagtgcg ttcagcggat
      721 tcctcagctc gtgcgagacg gtcgccagga atacgtcttt ggctcgactg gcttgctcgg
      781 cgacggcgag ggcacgacgc gccgaaagtt cgctttgctt ggcccgatcg tggagcctgc
      841 gtgagcgaac gagcgtcgcc acccgcgcga cgagctccgc cgaatcgaac tctttcgtga
      901 cgaaatcgtt ggcgcccgcc gcgagccctt ccagcaagtc gtcgcggccc gcggttgcgg
      961 tcaaaatcaa tattggcaga ctcgcctcgt cgtgcgactg ccgcagaaag cgacacacct
     1021 cgagacccga cacgtttggc atgtgccagt ccaagatgag cacgtccggc aatatccctt
     1081 gggtaacgcg ctcgatgacg gtcgcgccgt cgaaaaacag ctggacgtcg tgctgctgct
     1141 ccaagatcgt cttcgcgaac tcggcctcga gcggactgtc gtccacgacc caaatcttgg
     1201 ggcgtgcacc ggagcggttc gtgtcttcca aatcagctcg cttttcgttc gtgcgcgtgg
     1261 aagttaccgc atcgcgtatg agaacgcctg cgattcggca cttcgtaatt cccggacacg
     1321 actgaggcgt ttgtgcgaag cctcgatttg acccgtcgct cgagcgagcg tagtttgccc
     1381 cgctcgtgag ttgctcgatg gcatcgatct tggtcgtgga cgacagtcag gtcatgcgcg
     1441 acatggtcgt cgcgtgtctg cgcgcgattt ccgaaacgag cttcacgcat gcggcgagcg
     1501 gcctcgaggc gatcgagcgt ttgtcgctga agaagttcga cttgatggtg ttggatctga
     1561 acatgcccga catcggcggc atcgaggttt tggagttcgt gcgcggacaa gacgcgcttc
     1621 gaaggctgcc cgtgctgatc gtcaccaccc gcggcgacca agattctcgg caacgagtgc
     1681 tgtccgcggg ggcgtcggcg tttttggcca agccgtttac tccccaggcg atcatctccg
     1741 aggtcaacgc gctgctgtcc gcgcgcgctg aaccctcggc atgacggtcg aaacgaacct
     1801 caaggaattc ttcaccgcgt acctggtcga gatgggggag tacctggtgg ctgcgaacac
     1861 gcagctgctc gcgctcgaag cgtcgctgcg ggcgggggga gcgaacccac gagcagttcg
     1921 cgagaccttt cgcgccctcc acaccatgaa ggggctttcc gcgatggtcg gcgtcgaacc
     1981 gatcgtgggc gtcgcgcacc gcctcgaaga gtttttgcgc gattacgaca agcgcggaac
     2041 gaagccgccg ctcgcgagca tcgacgtgct gttgcggggc gtgcgcacca tcgagctctg
     2101 catcgaagag ctcggtagcg gaagtgagcc gcaccctatc ccggacaacc tgttccgaga
     2161 cttggaagca atcgagccgt ccggacagag cttggaagcg cccccacccg cgctctcctt
     2221 ggctccggat ctggtccgaa agctcgcgcc cttcgaactc gctcagctga cgcaagccga
     2281 gcagagtggc caacgcgcgc tccgcgccga cttctgtccg tcgcccgagc gagccgcggc
     2341 cggcttgtcg attgccaccg tcagggagcg tgtggcgaaa gtcgctgaga tcgtcaagat
     2401 acttccgctc tccgtcccgg tatctccgga cgcgccgggg ggactcgcgt tcgcgctctt
     2461 gctggtgacg agcgagagcg acgcggcgat cgccgatgcg atcggtgtcc cagccacgac
     2521 gctgagcgct ttgtgcacac cttcggcgga cgccggggcg gccttggatg tgcctttgcc
     2581 agccgaagat cggcaggagc cggttcggga tggcggggct ccgcagcgca gcagcctacg
     2641 cgtcgacgtg gctcgcctcg acgacgtgat ggagcggctg tcggcgttga tcgtgacgcg
     2701 ctttcgcatg gcgcgcgcgg tcgccactct cgaggccgcc ggcgccaaca ccgccgaagt
     2761 cacggctatt ctcggcgaga cggcccgcca attgcgcgac cttcgcgcag cggtggtggg
     2821 ggttcgcatg gtacccgtcg ccgatctcct ggagcgggtg ccgctgctcg ttcgaggcct
     2881 tcgccgccaa accaaccgac aagtgcggct cgagctcgtg aacgggaatg ccgagctcga
     2941 caagtcggtt tccgagcgtt tgtttcccgc gatcgtgcac ctggtgcgca acgcggtcga
     3001 tcacgcgatt gaaacacccg atgagcggct ccgccgaggt aagccgcaag aggggctcct
     3061 gcgcatcgtg tgtgcgtcgc gctccaacaa ccgcctggac gtcagcgtcg aggacgatgg
     3121 gcgcggcatc gacgccgagg ccgtcgcgcg gagagccggg cgcccc
//
LOCUS       DASTKS010000976         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1942825, whole genome shotgun sequence.
ACCESSION   DASTKS010000976 DASTKS010000000
VERSION     DASTKS010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942825"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFQ35_20585"
     CDS             <1..192
                     /locus_tag="VFQ35_20585"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933118.1"
                     /translation="RFTDDRARDVSENTRREVEKRLIAVNSPPEWVQCVREFVPRDEQ
                     AGGGWFDEDLPVGLVIKTI"
     gene            complement(304..798)
                     /locus_tag="VFQ35_20590"
     CDS             complement(304..798)
                     /locus_tag="VFQ35_20590"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9933119.1"
                     /translation="MTLGHVTHAQKGSVHVLRYFGTVNFLLAPGIQNFVDHLIEDGSV
                     SGLVFDLSGASSLDSTNLGLMARVNERLVSKGAANSVIISGNEDIDAVLRSMGFDQTF
                     ELLSGDADDGAPNVEPIQTPAPSSVELKRTMLDAHQALIRMSEAHRKEFEAVVACLER
                     DPES"
     gene            complement(795..1976)
                     /locus_tag="VFQ35_20595"
     CDS             complement(795..1976)
                     /locus_tag="VFQ35_20595"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HET9933120.1"
                     /translation="MTEEPAVLIVDDDEGVRRVFRRVLEKQGMRVVEANDGASGIARC
                     DEVAPGMVFLDLRMPGMDGLDVLSALVERHPETPVVVVSGQGTMDDAVQALRRGAWDF
                     VGKPLADNELLIRAARRGIERSQLLKQNREYGESLRSANQRLSAALRELRTDEQAARQ
                     LQFQLLPKEGLRIGDVSFNRRLFPSQLLSGDFLDYFPLTERYAGLYLADVAGHGAASA
                     FVTAILTTLVGKYRELLHARDDETILSPPRFLTSLDADLASLSIAKHVTMFYAVLDLA
                     SGRLAYCNAGAFPFPFLANGTRVAELEVSGRPLNLPGRGGFGEGEAEMTVGSRLLLLS
                     DGVLELAPKKTQRLRRDEIAELLSGAQTMEDLTRPLGLTESTALSDDIAVVFARREEN
                     P"
BASE COUNT          388 a          710 c          699 g          390 t
ORIGIN      
        1 cgcttcaccg atgaccgcgc gcgcgacgtc agcgagaaca cgcgccgcga ggtggagaag
       61 cgcctcatcg ccgtcaattc tcccccggag tgggtccaat gcgtgcgcga gttcgtcccg
      121 agagacgaac aagcgggtgg cggctggttc gacgaagatc tacccgtcgg cctcgtcatc
      181 aaaaccattt gagtggttac ggccggcgcc ttcttcacgc gatacgagca gagggcgcgc
      241 tgcgcgacca gggcatccat cgctcgggcg cagtcacatg ccgagcgtcc gtaaacggcg
      301 tgctcaggac tcgggatcgc gctcgaggca ggcaaccacg gcctcgaact ctttgcggtg
      361 cgcctcgctc atgcgaatca gcgcttggtg cgcgtcgagc atcgtgcgct tgagctctac
      421 gctcgaaggc gccggggttt ggatcggttc tacgttgggc gctccgtcgt ccgcgtcgcc
      481 ggacaggagc tcgaaagttt gatcgaagcc catgctgcgt agcaccgcat cgatgtcttc
      541 gttccccgag atgatgacgc tattggcggc gcccttgctc acgagccgtt cgttgactcg
      601 tgccatcagt ccgaggttcg tgctgtcgag cgaactcgcc ccgctgaggt cgaacaccag
      661 acccgacacg gagccgtcct cgatgaggtg gtcgacgaag ttttggatcc caggcgcgag
      721 caggaagttg acggtgccga agtagcgcag aacgtgaacg cttccttttt gagcgtgagt
      781 gacgtggccc agagtcatgg gttctcctct cgccgcgcga agacgacggc gatgtcgtcg
      841 ctcagcgccg tgctctcggt gaggccgagc ggcctcgtga ggtcttccat agtctgcgcc
      901 ccggaaagca gctccgcaat ctcatctcgt cgcaggcgtt gcgtcttttt gggagcgagc
      961 tcgagcacgc cgtccgagag tagaagcaat cggctaccca ccgtcatttc tgcttcgcct
     1021 tcgccaaagc cgccacgccc gggcagattc aacgggcggc cgctcacctc gagctcggcc
     1081 acgcgcgtcc cgttcgccag gaacggaaat ggaaaggcgc ccgcgttgca gtaggcgagc
     1141 cggcccgatg cgaggtcgag caccgcgtag aacatggtaa cgtgcttggc gatcgagagg
     1201 gaagcgaggt ccgcgtcgag gctcgtcaag aagcgcggcg gagacaagat cgtctcgtcg
     1261 tcgcgcgcgt ggagaagctc acggtacttg ccgaccagcg tcgtgaggat cgccgtgacg
     1321 aacgcggaag ccgcgccgtg gcccgcgacg tctgcgaggt agaggcccgc gtagcgttcg
     1381 gtcaagggga agtagtcgag gaagtcaccg ctcagtagtt gcgaggggaa caagcgccgg
     1441 ttgaacgaaa cgtccccaat ccggaggcct tctttgggca gtagctggaa ctgcagctga
     1501 cgagccgcct gctcgtccgt gcgcagctcg cgtagcgcag ccgagagccg ctggtttgcg
     1561 ctgcgcaagc tctctccgta ctcgcggttt tgcttcagca gctgggagcg ttcgattccg
     1621 cgtcgcgcgg cccggatcaa aagctcgttg tcagccaagg gcttcccaac gaagtcccag
     1681 gcgccgcgcc gtagcgcttg caccgcgtcg tccatcgtcc cctgaccaga gactactacg
     1741 acgggagtct cggggtgccg ctcgacgagc gcgctcagca catcgagccc atccatcccc
     1801 ggcatgcgca gatccaaaaa gaccatcccc ggggcaacct cgtcgcaccg cgcgatgccg
     1861 ctcgcgccgt cgttggcctc cacgacgcgc atgccttgct tttcgagcac ccggcggaag
     1921 acacgacgca cgccttcgtc gtcatccacg attagtacgg cgggctcttc cgtcatcgat
     1981 gactccaaac ctccgagtcg gcagagagcc taacattctg ccagcggtca tcgtggcgcg
     2041 cgtccccatc gtgcgcgacg gatttcgcca gcgtccaacc gccaacgacg cgtacgagtg
     2101 gccgcgagct ggggcgtgcc tccacgagcg cacggttcga cgacttctcc gccagccgaa
     2161 atcgttacgc gaaagcgtgt cgttttc
//
LOCUS       DASTKS010000977         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_155329, whole genome shotgun sequence.
ACCESSION   DASTKS010000977 DASTKS010000000
VERSION     DASTKS010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155329"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1034)
                     /locus_tag="VFQ35_20600"
     CDS             complement(186..1034)
                     /locus_tag="VFQ35_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9933121.1"
                     /translation="MADSGKKRRGAALSLAHVTKHFGSRSVLSNLSLEVRPGEFVAVV
                     GRSGSGKSTLVRVLCGLEEPSGGSLRLPERGDRGGRGGVRVVFQEPRLLPWKSVLENT
                     SFGIAARDAEGKRATREAALRALDSVGLRDRIHDYPRVLSGGQRQRVALARALLHEPS
                     LMLLDEPFSGLDALTRIEAQRLIESLWLERGFTALLVTHDVSEAVLLADRVLLLEEGA
                     FAATFEVNLPRPRPRDDVEVARIAANVLGRILASPEGGRARRPSEDHETVAQAFSESR
                     PKPAHA"
     gene            complement(1037..1822)
                     /locus_tag="VFQ35_20605"
     CDS             complement(1037..1822)
                     /locus_tag="VFQ35_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9933122.1"
                     /translation="MNLREAVGRGVLPWIAPVGLGALWQALSQSGVISQNLLPPPLDV
                     GKAALRALRYQSLLTHLAVSAGRAALGLFIGSALGLGLGVLTGLSRTLQLLLDGPLQM
                     IRAVPALALVPLVISWFGLGETAKIFIVAITVVFPVYLNTFHGVRSVDPHLLEMARVY
                     DVHGLALYTHVVLPGALPSVLVGFRFALGMSWLVMIVAETLGASSGLGYVAATAREFM
                     QMDVLVLTIVLWALLGKATDVLARRLERALLPWQPRSQRLSAD"
     gene            complement(1819..2835)
                     /locus_tag="VFQ35_20610"
     CDS             complement(1819..2835)
                     /locus_tag="VFQ35_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9933123.1"
                     /translation="MNRKIFIVRLTVALVVWFASACQKQRDPGQPSPGAVRTVSADPK
                     PITQVHLGFQKIGAPFLLKSRSAELDKHLAEASAKADWKEFKHGPALLEAVNAKEVDI
                     GYVGETPPVFAQAAGVDFVYVANDPPAPKAEAIVVRKDSPVRTPADLKGKKVALNRGS
                     NVHYLLLKALESSKLGVNDIEVVYLAPGDARPAFDSGQVDAWVIWDPFLAAAELSGSR
                     VLVDGEGLVDNHFFYVARRDFAEQHPELLRIVLDEFRSLSDWESRNPEEASKILAGSS
                     AISYEALLLAEKRHSYGVLPITGEVLETQQRIANAFRKLELIPKDITTRDAFVPAAVY
                     AKRP"
     gene            complement(2857..3774)
                     /locus_tag="VFQ35_20615"
     CDS             complement(2857..3774)
                     /locus_tag="VFQ35_20615"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HET9933124.1"
                     /translation="MTLQHNYSYKATLQDSLKVNWRVDDLIGGDKTLDFTKPFLPESL
                     VRVEGLPSLSPRERLTLNQIRGASYLHVFGLVEEFILPFAVERARDGIHASKDRVRAL
                     LTFAEEEAKHQQLFQRFSEEFRRGFPTPIEYIGPATAIAENVLANSPLSVGILILHLE
                     WLTQRHYLESIKTDQALDPQFVSLLKHHWQEEAQHAKLDTLVVEELASRASPAEIEKA
                     VEDFLTIGATLEGGLQQQLAYDLDGLERFIGRKFSTEERIRISAQQLPSYRWTFLVSG
                     LEQENFVRAIRDLSPAGLERVQATARALS"
     gene            3998..>4539
                     /locus_tag="VFQ35_20620"
     CDS             3998..>4539
                     /locus_tag="VFQ35_20620"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9933125.1"
                     /translation="MTNPNQTKTNQGVPAFPKPERPAHVIRSDGEAITIAKELAERFA
                     RGAIERDRERKWPIEELDAFSQSGLWSINVPKQFGGPELSYVTLTRVIEIISAADPSL
                     GQIPQNHLGVVAAIRTVSEPAQQKFLFGEILKGVRLGNAFSEFGSKRAVDFETRFVDA
                     GDHVVVNGKKFYSTGALLAHL"
BASE COUNT          856 a         1406 c         1454 g          823 t
ORIGIN      
        1 gcggcgcttc caactcgctc tcatgagcga ccaagagcca actcgttcgc caaatcgtct
       61 ggagttcggc gtcgaagatg tcttgctccg tgtaaattcg accgtcgacg aacggcagcg
      121 tggggccgcc gagtttctgt cgccactcgc gggtgctctc tttggtcatg attgtcgttt
      181 ctccctcagg catgcgcagg ctttgggcgc gactccgaaa aggcttgcgc cacggtttca
      241 tgatcctcgg agggccgccg cgctcggccc ccttctggtg aagccaagat tcgaccgagc
      301 acgttcgcag cgatgcgagc aacctcgaca tcgtcgcgag gtcgcggccg cggcaggttg
      361 acttcgaaag tcgccgcgaa cgcgccttcc tcgagcagga gcacgcgatc ggcgagcagt
      421 accgcttcgc tcacgtcgtg agtcacgagg agcgcggtaa agccgcgttc gagccacagc
      481 gactcgatga gccgctgcgc ttcgattcga gtcagggcat cgaggccgct gaacggctcg
      541 tcgagcaaca tcaggctcgg ctcgtggagc agtgcccgag cgagcgcgac gcgttgccgc
      601 tgacctccgg agaggacgcg tgggtagtcg tgtatccggt ctcgcaggcc gaccgaatcg
      661 agagctcgaa gcgctgcctc acgagtcgcg cgcttgcctt ccgcgtccct tgccgcgatc
      721 ccaaacgatg tattctcgag gacgctcttc cacggcaaca gtcggggctc ctggaaaacg
      781 acgcgtacgc cgccgcggcc acctcggtct ccgcgctcgg gaagcctcaa gcttccgccg
      841 ctcggctcct cgaggccgca taggacgcgc accaacgtgc tcttgccaga accgctgcgc
      901 ccgacgaccg cgacgaattc gcccggtcga acttcgagcg agaggttcga aagcaccgac
      961 cgcgagccga agtgcttcgt cacgtgagcc aagctgagcg ccgcgccgcg gcgctttttg
     1021 ccggagtccg ccatgatcaa tccgcgctca atcgctgaga tctgggttgc cagggtagaa
     1081 gggccctttc gagacgccga gccaggacgt ccgtggcctt tccgagcaat gcccacaaga
     1141 cgatcgtcag gactagtacg tccatttgca tgaactcacg cgcggtcgca gcgacgtacc
     1201 cgaggccgct gcttgctccg agcgtttcgg cgacgatcat gaccagccac gacattccga
     1261 gtgcaaaccg gaacccaacc agcaccgagg gcagcgcgcc gggcaagacc acgtgcgtgt
     1321 acagtgcgag cccgtgaacg tcgtagactc gagccatttc gagtaagtgc gggtccacgc
     1381 tgcggacgcc gtggaacgtg ttcaggtaca ccgggaacac gaccgtgatc gcgacgatga
     1441 agatcttcgc cgtctctccg agcccgaacc acgagatcac gagcggcacc agcgcgagcg
     1501 ccggaacggc ccggatcatc tgcaaagggc cgtcgagcaa cagctgcagc gtccgagaca
     1561 accccgtcag caccccgagc ccgagcccca gcgcggagcc gatgaaaagt ccgagcgcgg
     1621 cgcggccggc gctgacggcc aggtgggtga gcaagctttg atatcgaaga gcgcgcagcg
     1681 ccgctttgcc gacatcgagc ggcgggggca agaggttttg tgagatgaca cccgactgcg
     1741 agagggcttg ccagagcgcc ccgagcccga ccggggcaat ccagggcagt acaccgcgac
     1801 cgaccgcttc gcgcagattc atggcctctt tgcgtaaacg gcagcgggca cgaaggcgtc
     1861 ccgagtggtg atgtccttgg ggatgagctc gagcttacgg aaggcgtttg cgatgcgctg
     1921 ctgggtctcg agtacctcgc cggtgatcgg caagactcca taagagtggc gcttctccgc
     1981 gagcaacagc gcctcgtacg agatcgccga agaccccgcg aggatcttgg atgcttcttc
     2041 cggatttctg ctttcccagt cgctcaggct cctgaactcg tcgagcacga tgcgcaaaag
     2101 ctctggatgc tgctcggcga agtcgcggcg cgcgacgtaa aagaagtgat tgtcgacgag
     2161 tccctcgccg tccacgagta cgcgagagcc cgacaactct gccgctgcga ggaacggatc
     2221 ccagatcacc caggcgtcga cctgtccgct gtcgaaggcc gggcgcgcgt ccccgggcgc
     2281 gaggtacacc acctcgatgt cgttcactcc gagctttgag ctctcgagtg ctttcaacaa
     2341 caggtagtgt acgttggagc cgcggttcag tgcgaccttc ttccccttca aatcagcggg
     2401 ggttctgacg ggcgagtctt tccgaaccac gatcgcctcg gccttggggg caggcggatc
     2461 gtttgcgacg tagacgaagt cgacgccggc ggcttgtgcg aacacgggcg gtgtctcgcc
     2521 gacgtagccg atgtcgacct ccttcgcgtt gaccgcttcg agcaacgctg ggccatgctt
     2581 gaactctttc cagtccgcct tggcagaggc ttcggcgagg tgcttgtcga gctctgcgct
     2641 gcgggacttc agcaagaagg gcgcgccgat cttctggaag ccgaggtgca cctgcgtgat
     2701 cggcttgggg tctgccgaca cggtccgaac cgcgccgggc gacggttgtc ccggatcgcg
     2761 ctgcttctgg catgccgaag cgaaccaaac cacgagcgcg acagtgaggc gcacgatgaa
     2821 gatcttgcga ttcatgccat tccggagggg gagacctcaa ctcaaggcgc gtgccgtggc
     2881 ttggacgcgt tcgagaccgg ctgggctcaa atcgcggatg gctcgcacga agttctcttg
     2941 ctcgaggccc gagaccaaga acgtccagcg gtacgacggg agttgctgcg ccgaaatgcg
     3001 tatgcgctct tccgtcgaga acttgcggcc gatgaagcgt tcgagaccgt cgaggtcgta
     3061 tgccagttgt tgctggagtc cgccttcgag tgtcgcaccg atcgtcagga aatcttcgac
     3121 ggccttctcg atctctgctg gcgacgcgcg gctcgcgagc tcttcgacga cgagggtgtc
     3181 gagctttgcg tgctgcgctt cctcctgcca atggtgtttg aggaggctca cgaactgcgg
     3241 gtcgagggcc tggtccgtct tgatgctctc gagatagtgc cgttgagtga gccactcgag
     3301 gtgcaaaatc aggattccga cgctgagcgg gctattggcg agcacgttct cggcgatggc
     3361 agtcgcggga ccgatgtact cgatgggtgt aggaaaaccg cgtctaaact cttcagaaaa
     3421 gcgttgaaag agctgctggt gcttggcctc ctcttcggcg aaggtcaaaa gggcacgcac
     3481 tcggtctttg ctagcgtgaa tgccgtcgcg cgccctctcc accgcgaagg gaaggatgaa
     3541 ttcttcgacc aagccgaaga cgtgcaggta gctcgcgccg cgaatttggt tcagcgtgag
     3601 tcgctcgcgc gggctgaggc tcggaaggcc ttcgacgcgg acgagggatt cgggcaaaaa
     3661 cggcttggtg aagtccaggg tcttgtcgcc gccgatcaag tcgtcgactc gccagttgac
     3721 cttcaaagag tcctggagcg tggccttgta actgtagttg tgctgcagcg tcatcgttga
     3781 cgacctcctc gaacgaagcc acggaacgag cacggaacat gccaagcgcg aaacgccgtt
     3841 ccgggcggga cttggcctca agggtcgagt ggactgttgt tacagcagca gccctccagc
     3901 atcgggtgca tgagaagaag catcggagcc gtgacggtcg tgccctcgga tgcgggtgac
     3961 aatggcacga agcgtgctga gcgcgactca agcagccatg accaatccga atcagaccaa
     4021 gacgaaccaa ggggtacctg cctttccaaa gccggagcgg cccgcgcacg tcattcgcag
     4081 cgacggcgag gcgatcacca tcgcgaagga gctggccgag cgcttcgcga ggggcgcaat
     4141 cgagcgcgat cgggagcgca aatggccgat cgaggagctc gatgcctttt cccagagcgg
     4201 cctctggtcc atcaacgtgc ccaagcaatt cggcgggccc gagctctcgt acgtgacgct
     4261 cacgcgcgtc atcgagatca tctcggccgc ggatccttca ctcggacaaa ttccgcaaaa
     4321 tcacctcggg gtagtcgccg caattcggac cgtctcggag cccgctcaac agaagttctt
     4381 gttcggcgaa atcctgaaag gggtgcggct cggcaacgct ttctcggagt ttggctcgaa
     4441 gcgcgccgtc gacttcgaga cgcgcttcgt cgacgccggt gaccacgtcg tcgtcaacgg
     4501 caagaagttc tattctaccg gagcgctgtt ggcgcacct
//
LOCUS       DASTKS010000978         6811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1943004, whole genome shotgun sequence.
ACCESSION   DASTKS010000978 DASTKS010000000
VERSION     DASTKS010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943004"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VFQ35_20625"
     CDS             complement(<1..1421)
                     /locus_tag="VFQ35_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933126.1"
                     /translation="MVSKRDAVNLALQAALDSWNWRPPPNPDALPLVESAASRGLFTD
                     SSVTVFSGDLEEWVTYLRLSQGERYEPAISVRAHVQPELRLLVFDAESEDARWLSDIL
                     NNAYRRLAKRVEGKPYVARALAELLFEGGASRLPTLVRLSPHVPSDGTRESDGESPSG
                     DVRVVLHSRLARYVREALANAGDASADERLGYAWPLISRVVSALAYPASARDRFAVKI
                     RLFAKMSYVALNVLYESRKSGELVPGVAGAVYENMVSASSGVARNRLTERHPYFRMLS
                     RLGFMLRAEPYSHYAGEAEIGVREYLDSVYLRLSLAGAMPGVAEAAQPMSFERKGRKV
                     RTERPEVGRFGILDRDDLDAWKTVADPFQDLGFALLRQVGMGEFGRVYEALNDNNPNW
                     PARVALKVDRIIGKKKNAILEAEAAMEVGRRLAGAHHIIRIYDTGKLKGQRFTYHVLQ
                     LIDGDTLDDLVGVTGTEHASVSRP"
     gene            1586..2905
                     /locus_tag="VFQ35_20630"
     CDS             1586..2905
                     /locus_tag="VFQ35_20630"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9933127.1"
                     /translation="MSSAVELIQKKRDGGELTASEVAQLVGGLLSGEIADYQLSAFLM
                     ATYFRGMTPRETIALTEAMLHSGKVLHLRSVTRPKVDKHSTGGVGDKISIPLAPLVAS
                     CGAAVPMVSGRGLGHTGGTLDKLESIPGFRVNLTASTFERIVREVGTAMIGQTAEIAP
                     ADRRLYALRDVTATVESIPLIVASILSKKLAEGIDALVLDVKAGRGAFMKDVKSAREL
                     ARALVAVGKRAEKRVAALVTDMSNPIGLTIGNALEVRESIEILQNRGPADTRELTLEL
                     GVEMLRTAGVERSSARAKGLLENALADGSAFECFCRMVRAQKGDVRAVERPERLPRAK
                     FELAVAAPRSGVVQAIDALELGLCSVSLGAGRARAEDDVDPGAGIRINAPIGTRVARG
                     EPLAFLYASTRARAEATAERAGAAFRLAPRAKKPEPRVLERVGSGRS"
     gene            2916..3698
                     /locus_tag="VFQ35_20635"
     CDS             2916..3698
                     /locus_tag="VFQ35_20635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9933128.1"
                     /translation="MAFDIRRFLREQALIIDAEYRTSNLPSHKAALVLVTAALALVLP
                     KYFGSPDAFNRMQWAQELFAPLPYPELHPQLYWSAFKLLHYGLLPYLCLKLVLKGDLR
                     SHGVRFVWEPKVWLLYASMLLIVLPLTYVASGSSSFLRTYPKYAGAGNSLGELLCWEA
                     AYAFQFLMLEFFFRGFLTFALARYIGSLAVFVMVVPYAMIHLSKPFAECLGSIIAGTV
                     LGTVALRTGSIYGGVAVHCVVAWGMDLFALAAKGKLLQLFQQ"
     gene            complement(3793..5841)
                     /gene="metG"
                     /locus_tag="VFQ35_20640"
     CDS             complement(3793..5841)
                     /gene="metG"
                     /locus_tag="VFQ35_20640"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9933129.1"
                     /translation="MTRRILVTSALPYANGHIHLGHLVEYIQTDIWVRFQRLRGNDAI
                     YICADDTHGTAIMMRARQEGRSEEAVIQEMSTAHQRDFAAFQVSFDHYGSTHTAENRE
                     LCGEFWSGLQQANLVVTRDITQLFDTKAGVFLADRFVKGKCPRCGSADQYGDSCDKCG
                     STYLATELIEPVSTLTGTRPELREAKHLFVQIEPMHAFLEDFVNTPGRLQPEMANYLK
                     GHFLAEPLRDWDISRPAPYFGFEIPDEPGHFWYVWFDAPIGYLGSTHQYCKKHGQSLD
                     DWWRNPDVEIYHFIGKDITYFHTLFWPVMLKATGYSLPRRVQVHGFLTVEGEKMSKSK
                     GTFILASTYAKELDPAWLRYYYASKLGPRVEDLDIQIDEFVSKVNSDLVGKVVNLASR
                     SARFVESTGLSAAYPDDGGLFAEAARRGEEIAEAYETCDYARAMRVIMALADRANEYV
                     DRAEPWKLKKDASRAAELQDVCSIILNLYRQIVIYLSPVLPALSTASEQLLNTRLSRW
                     SDAATPLVATRVAPFSHLMQRVDPNKVKAVIEQSREAAPAEASAPSEPAKTFDDDGAA
                     LAAEPLAAECSIDDFQKVDLRVARVINAEHVPEAKKLLKLTVSLGGGTTRTIFAGIKA
                     AYEPEKLVGRLIVVVANLAPRQMKFGLSEGMVVAAGSGGSEVFVLSPDSGAKPGHRLH
                     "
     gene            5917..6282
                     /locus_tag="VFQ35_20645"
     CDS             5917..6282
                     /locus_tag="VFQ35_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933130.1"
                     /translation="MCFAAVAQARSARAADDFFGPDKALHFGVSAGLAATGYAGSALV
                     LERPWQRALAGATFALSAGIAKELWDLSGHGDPSWKDLAWDGIGTGVGVGLALGVDLL
                     LFRHRGDAAPSRAALVIRF"
     gene            6610..>6811
                     /locus_tag="VFQ35_20650"
     CDS             6610..>6811
                     /locus_tag="VFQ35_20650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933131.1"
                     /translation="MADDDALVRGALVRSLTALGYDVTAAANGLEATELLGRGQFEVI
                     VSDIAMPEIDGIQLLKAIRQRDP"
BASE COUNT         1147 a         2187 c         2221 g         1256 t
ORIGIN      
        1 ggtcggctga cgctcgcgtg ttcggtgccg gtcacgccga cgaggtcgtc cagggtgtcg
       61 ccgtcgatca gttgaagcac atggtaagtg aagcgctggc ccttgagctt cccggtatcg
      121 tagatgcgaa tgatgtggtg agcgcccgcc aatcggcgcc caacttccat cgccgcctcc
      181 gcctcgagaa tcgcgttctt cttcttgccg atgatgcgat cgaccttgag ggcgacgcgc
      241 gccggccagt tggggttgtt gtcgttgagc gcttcgtaaa cgcgcccgaa ctcccccatg
      301 ccgacttgcc gcaagagggc gaaccccaga tcttggaaag ggtcggcgac tgtcttccac
      361 gcatcgagat cgtcgcggtc gaggatcccg aagcgtccga cctccgggcg ctcggtgcgc
      421 actttgcgac ccttgcgctc gaaggacatg ggctgcgcgg cctcggcgac accgggcatc
      481 gcgccggcaa ggctgagcct gaggtacacc gagtccaggt actcgcgcac gccgatttcg
      541 gcctcgcccg cgtagtgcga gtaaggctcg gcgcgcagca tgaagccgag gcgtgacagc
      601 atgcgaaagt acgggtgccg ctcggtcaaa cgatttcggg caacgccgct gctcgcgctg
      661 accatgtttt cgtagaccgc ccccgccacg cccggcacga gctcgccgga cttgcggctc
      721 tcgtacagca cgttcagcgc gacgtagctc atcttcgcga agaggcggat cttcaccgcg
      781 aaccgatctc gagccgacgc cgggtacgcg agcgcggaaa cgacgcgcga gatgagcggc
      841 catgcgtagc cgagtcgctc gtcggcgctg gcgtcgcccg cgttcgcgag ggcctcccgg
      901 acgtaacgag cgagccgcga gtgcagcacc acccgcacat cgccgctcgg gctctcgccg
      961 tcgctctcgc gcgtgccgtc gctcggaacg tgcgggctga gacgcacgag cgtcggcaac
     1021 cggctcgctc cgccctcgaa taaaagctcc gcgagagcgc gggcgacgta cggcttacct
     1081 tcgacccgct tcgcgaggcg gcgataggca ttgttgagga tgtcagaaag ccaacgggcg
     1141 tcttcgctct ccgcgtcgaa cacgagcaac cgcagctccg gctgaacgtg ggctcgcacg
     1201 gagatcgccg gttcgtagcg ctcaccctga ctgaggcgca ggtaggtgac ccactcttcg
     1261 aggtcgccgc tgaaaacggt gactgacgag tcggtgaaga gcccccggga tgccgccgac
     1321 tcgacgagag ggagtgcgtc cgggttcggc ggcggacgcc aattccagga atccagtgcg
     1381 gcctgcaggg cgaggttgac tgcgtctcgt ttgctcacca tgcgcgctcg cgaggtgctc
     1441 ctgaagctac cacgagttcg acgcggaaac ctcgcccgag ccgcgcaatt tccgcagttt
     1501 ttctgctcga tcgcgggcct agaaggcgtc gcgtcaggct tcttctcttt gcggacctcc
     1561 gcgagtccgc tagcttcccg cccgaatgtc gagcgcggtc gagctcattc agaagaaacg
     1621 cgacggcggc gagctcacgg cgagtgaagt cgcccagctc gtcggcggac tgctgagcgg
     1681 cgaaattgcc gactaccagc tcagcgcatt cctgatggcg acgtacttcc gcgggatgac
     1741 cccgcgcgaa accatcgcgc tcaccgaagc gatgctgcac tcgggcaagg tacttcatct
     1801 ccgaagcgtc acgcgcccga aagtcgacaa acactcgact ggtggcgtcg gggacaagat
     1861 cagcattccg ctggctcccc tcgtcgcctc gtgcggcgct gccgtaccga tggtgtcggg
     1921 acggggtctc ggccacacgg ggggaacgct cgacaaactc gaatcgattc caggttttcg
     1981 agtgaacctt acggccagca ctttcgagcg catcgtgcgt gaagtcggca ccgccatgat
     2041 cgggcagacc gccgaaatcg cgcccgccga ccggcgcctg tacgcccttc gtgacgtgac
     2101 cgcgacggtg gagtcgattc cgctcatcgt tgccagcatc ctgtcgaaga agctcgccga
     2161 aggcatcgat gcgctcgtgc tcgacgtgaa ggccgggcgc ggagccttca tgaaagacgt
     2221 gaagtctgct cgcgagcttg ctcgagcgct cgtcgccgtc ggcaagcgcg ccgaaaagcg
     2281 tgtcgccgcc ctcgtcacgg acatgtcgaa cccgattggc ctcacgatcg gcaacgcgct
     2341 cgaggttcgt gagtccatcg agattttgca gaatcgtggc cccgctgaca cacgcgagct
     2401 cactctcgag ctcggggtcg agatgctgcg aacggccgga gtggaacgct cgtccgcgcg
     2461 cgcgaagggt ctcctggaga atgcgctcgc ggacggctcg gcgttcgagt gcttctgtcg
     2521 catggtgcgc gctcaaaaag gcgacgtgcg tgcggtcgaa cgacccgagc gactgcctcg
     2581 agcaaagttc gagctcgcgg tcgccgcgcc ccggagcgga gtggttcaag ccatcgacgc
     2641 gctcgaactc gggctgtgct ccgtctcact cggggcgggg cgggcgcgcg ccgaagacga
     2701 cgtcgacccc ggggcgggca ttcgaatcaa cgcgccgata ggtacacgcg tcgcccgcgg
     2761 agaaccgctc gcgtttctct atgcctcgac ccgcgcgcgc gcggaagcga cggcggagcg
     2821 ggcaggcgcc gcgtttcggc tagcgccacg agccaaaaag ccggagccac gagtgctcga
     2881 gcgcgtcggc agcgggagat cttgagggtc caaaggtggc attcgacatc cggcgcttct
     2941 tgcgcgaaca agctctcatc atcgacgccg agtatcggac gagcaacctg ccgagccaca
     3001 aggctgcgct cgtgctcgtt accgccgcgc tcgccctggt gttaccgaag tacttcggct
     3061 ctcccgacgc gttcaaccgc atgcagtggg ctcaggagct cttcgcgcca ctgccctatc
     3121 ccgagctgca tcctcagctc tattggtccg cgttcaagct gctccactac ggactgctcc
     3181 cctacttgtg cctgaagctc gtgctcaaag gcgacctgcg ctcgcacgga gttcgcttcg
     3241 tgtgggagcc caaggtttgg ctgctctacg cgagcatgct gctcatcgtg cttccgctca
     3301 cgtacgtggc gtcgggctct tcgtcgttct tgcgcacgta cccaaagtac gcgggcgcgg
     3361 ggaactcgtt gggggagctt ctctgctggg aagcggcgta cgcctttcaa ttcttgatgc
     3421 tcgagttctt tttccgcggg tttctcacgt ttgcgctcgc ccgctacatc ggttcgctcg
     3481 ccgtgttcgt catggtcgtg ccctacgcga tgatccactt gagcaagccg tttgccgagt
     3541 gcctgggctc gatcatcgcc ggcacggtgc tcgggacggt cgcgttgcgc accggctcga
     3601 tttacggcgg cgtggccgtc cactgcgtag tcgcctgggg gatggatctc ttcgcgctcg
     3661 ccgccaaggg taagctgctt cagctctttc agcagtgagc tcgctttcgc ggggggggtg
     3721 acgcctacgc acacccctgc gcgagacatg gtcgcttagc agggcgttga aaaacgccct
     3781 gcgtcaacac ccttagtgga ggcgatggcc gggcttcgca ccgctgtcgg gcgacagcac
     3841 gaaaacctcc gaacctccgg agccggccgc gacgaccatc ccctcgctca gcccgaactt
     3901 catctggcgc ggcgcaaggt tcgcgacgac cacgatgaga cgaccgacga gcttctcggg
     3961 ctcgtacgcg gctttgatgc cggcgaagat cgtgcgcgtc gtgccgccgc ccaggctcac
     4021 cgtgagcttc aagagcttct ttgcctcggg cacgtgctcg gcgttgatga cgcgcgccac
     4081 gcgcaagtcc accttttgaa aatcgtcgat gctgcattcc gcggcgagcg gctcggcggc
     4141 aagcgcggct ccatcgtcgt cgaacgtctt ggcaggctcg ctcggggcag aggcttcggc
     4201 gggggcggct tcgcggcttt gttcaatcac ggcttttacc ttgttgggat ccactcgttg
     4261 catcaggtgt gagaacggcg cgacgcgcgt cgcaacgagc ggtgttgcgg cgtcgctcca
     4321 acgcgacaag cgcgtattca agagctgctc gctcgccgtc gacaacgccg gcaagacggg
     4381 cgaaagatag atgacgatct gacggtacag gttcaagatg atgctgcaca cgtcctggag
     4441 ctcggctgcg cgggaagcat ccttcttgag cttccagggc tcggcgcgat cgacgtactc
     4501 gttggcgcga tcggcgaggg ccatgatcac gcgcatcgcg cgcgcgtagt cgcacgtctc
     4561 gtaggcctcc gcgatctcct cgccccggcg cgcggcttcg gcgaagagcc cgccgtcgtc
     4621 gggatatgcc gcggagaggc ccgtgctctc gacgaagcgc gcgctccggc tcgcgaggtt
     4681 caccaccttg ccgaccaagt cggagttcac tttgctcacg aactcgtcga tttgaatgtc
     4741 caagtcttcg acgcgcgggc cgagcttgct cgcgtagtag taacgaagcc acgcggggtc
     4801 gagctccttc gcgtacgtgc tcgcgaggat gaaggtgccc ttcgacttgg acatcttctc
     4861 gccttccacc gtgagaaacc cgtgcacttg aacccgtcgc ggcaggctgt agccggtcgc
     4921 cttgagcatc acgggccaaa agagggtgtg gaagtaggtg atgtctttgc cgatgaagtg
     4981 atagatttcg acgtcggggt tccgccacca gtcgtcgagc gactggccgt gcttcttgca
     5041 gtactggtgg gtggagccca agtagccaat cggcgcgtcg aaccagacgt accaaaagtg
     5101 gcccggctca tcggggatct cgaagccgaa gtacggcgcc ggacgcgaaa tgtcccaatc
     5161 gcgcagaggc tcggccagga agtggccttt caggtagttc gccatctcgg gctggaggcg
     5221 acccggcgta ttcacgaagt cctccaagaa ggcgtgcatc ggttcgattt ggacgaacag
     5281 gtgctttgcc tcgcggagct cgggtcgagt tccagtcagg gtgctgaccg gctcgatgag
     5341 ctccgttgcg aggtacgtgc tgccacattt gtcgcaagag tccccatact ggtcggctga
     5401 gccgcagcgc ggacacttcc ctttgacgaa gcggtcggca agaaaaacgc ccgccttcgt
     5461 gtcgaagagc tgagtgatgt cgcgggtgac gacgaggttc gcctgctgga gcccgctcca
     5521 gaactctccg caaagctcgc ggttttcggc cgtgtgggtg ctaccgtagt gatcgaagct
     5581 cacctgaaaa gcggcgaagt cgcgctggtg ggcagtgctc atctcttgaa tcacggcctc
     5641 ctcgctgcgc ccctcttgcc gcgcgcgcat catgatcgcg gtgccgtggg tgtcgtccgc
     5701 gcagatgtaa attgcgtcgt tgccgcgaag acgctgaaat cggacccaga tgtcggtttg
     5761 gatatactcg accaaatggc ccaaatggat gtgaccgttg gcgtaaggca gtgcgctggt
     5821 gaccaggatg cgacgagtca tgagcagagc gccgtcatag cattccgtcc ggcgggaagc
     5881 gcacggaagc tacggagcag gctcctcgcg ttcgcggtgt gcttcgcggc cgtcgcgcaa
     5941 gctcgtagcg cgcgcgccgc ggacgacttt ttcggcccgg acaaagccct gcacttcggc
     6001 gtctcggccg ggctcgctgc cactggttac gctggctcgg ccctggtctt ggagcgtccc
     6061 tggcagcggg cgctcgccgg cgcgaccttc gccctgtccg ctggaatcgc gaaggagctt
     6121 tgggacctgt ccggacacgg cgacccctcg tggaaggatc tcgcctggga cgggattggt
     6181 accggcgtcg gcgtggggct cgcgctgggc gtggatttgc tgctgttccg ccatcgaggc
     6241 gacgccgctc cgagccgagc cgcgctcgtg attcgcttct agcgacccgc ttcgtccgag
     6301 gtcggagtga gggagcgtgg cggtcccgac ggcacgacac gagacccgag gattgcccat
     6361 cggctcttcc cgccgaaaag cccggctcgc gccttccgca cgacgagctc cctcaccccg
     6421 agagatctga cgaatgatgg gacacgagcc gtccccctcg caccgtttct tctaaaaagc
     6481 tcctgtgagg ttgaggggat cgaaacctga ccgttagctc tggaagcatt cagaccccag
     6541 agaacgatgg agaactttga gcgagacgta gagcgcgccg cagcaacttc gtcccgaggg
     6601 aaagtgctgg tggcagacga tgacgcgctc gttcgcggag ccctcgtgcg cagcctgacc
     6661 gcgctgggtt atgacgtgac cgcggccgcg aacgggctcg aggccacgga gctcctcggc
     6721 cggggacagt tcgaagtcat cgtgagcgac atcgcgatgc ccgaaatcga cggcattcag
     6781 ctcttgaaag ccatccgcca gcgcgaccct g
//
LOCUS       DASTKS010000979         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1496503, whole genome shotgun sequence.
ACCESSION   DASTKS010000979 DASTKS010000000
VERSION     DASTKS010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496503"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFQ35_20655"
     CDS             <1..467
                     /locus_tag="VFQ35_20655"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET9933132.1"
                     /translation="TTPYPLVADCRFALVLAEIGPVLRRNGVRVVEFSNSHAYRNTHF
                     GKLSLHANGLALDLHRIQVEEGDALSVQEDFRRRLGARGCREDAPALNRLACELRATG
                     LFSELLTPDYNADHRDHFHLGIARKNAPFDAHERGGLAGSPGFGSNGWRKPS"
     gene            485..985
                     /locus_tag="VFQ35_20660"
     CDS             485..985
                     /locus_tag="VFQ35_20660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9933133.1"
                     /translation="MMAESNLSEGTGSEADAVKVPVADMDNAERFYVGALGAERLERF
                     DAPTGVKRRASGAAAQKRRASPLSLRLGARARFDLCLHEPSSSAAEAISLAVDGPELD
                     ALRAHLVSAGVPVDGPRGLGVEGHAALYFIDPFGNRVELVTAEYPGSASLGSPSWETL
                     AYEWRG"
     gene            complement(1021..1932)
                     /locus_tag="VFQ35_20665"
     CDS             complement(1021..1932)
                     /locus_tag="VFQ35_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9933134.1"
                     /translation="MPDWDDLRYLLAVARAGSASAAARTLGVAQTTVSRRIAALEDEL
                     RVPLFVATATGQSLSPEGQRVLTHVERMERDALAALQALAGRGGEVAGPVCVTASEWM
                     IQRVIGPIVGPLLARYPELELELLAEPKHLNLQRRDADIAIRPSRFEQPDVVQREVAT
                     IGFGLYASDAYLASHGAPDFERQGEGHDLIAMSASLGKVPDVDWLPTIAARARVVARL
                     NAREPMATMAAAGVGFTCLPRFVGDATPGLRLLQTPGAPPSRKLWLGVHRDARSVKRV
                     RATADFLAERFSHLQAALCPDSPGDGR"
     gene            2039..2821
                     /locus_tag="VFQ35_20670"
     CDS             2039..2821
                     /locus_tag="VFQ35_20670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET9933135.1"
                     /translation="MQRREFLVHGATGVLIGCSPALSVAPASPRSKPEARAPIRGVCF
                     DLFTLFDPRSVVEVARAISPERAVALCEAWRVRQFEYAPLRVAAGRYVDFRVVTKDAL
                     EYAARSQAVHLTDAQREELVDAYSRLEPWPDARATLLAFRSAGLRLSPLANYSPRMLS
                     RLLENSGFAELFDAQISTDAARTFKPDPRAYALGVATLKLPREQIAFAAFGGWDAAGA
                     KWFGYPTFWVNRLGVPHEELAPGFDSTGPSLAELAAFVRDWA"
BASE COUNT          468 a         1042 c         1021 g          479 t
ORIGIN      
        1 ctacgacccc gtaccccctc gtcgccgact gccgcttcgc gctggtcctc gccgagatcg
       61 gccccgtgct gcggcggaac ggcgtgcgcg tcgtcgagtt ctcgaattcg cacgcgtacc
      121 ggaacacgca ttttggcaag ctcagcttgc acgcgaacgg cctcgcgctg gatcttcatc
      181 gtatccaggt cgaagagggc gacgcgctca gcgttcaaga agacttccgt cgcaggctcg
      241 gcgcgcgcgg gtgccgggaa gacgcgccgg cgctgaaccg actcgcgtgt gagctacgcg
      301 ccacggggct cttcagcgag ctcttgacgc ccgactacaa cgcggatcac cgcgatcatt
      361 ttcacctcgg catcgcgcga aagaacgcgc cattcgacgc tcacgagcgc gggggcctcg
      421 cgggctcgcc gggctttggc tcgaacggct ggcgcaagcc gagctgaaga gaagggagga
      481 ccgcatgatg gctgaaagca acctgagcga aggaacgggc agcgaggcgg acgcggtcaa
      541 agtaccggtc gccgacatgg acaacgcgga gcgcttttac gttggagcgt tgggcgccga
      601 gcgattggag cgcttcgatg cgccgacggg agtgaagcgc cgcgcgtctg gggcggcggc
      661 gcaaaagcgc cgcgcgagtc ccttgagctt gcggctcgga gcaagagcgc gcttcgacct
      721 gtgtttgcac gaaccgagct cgagtgcggc agaggcgatt tccctggccg tagacggccc
      781 cgaactggac gcgctgcgcg cgcatctggt atccgccggt gtgccggtgg atgggccgcg
      841 agggctcggt gtagaagggc acgctgcgct ctacttcatc gatccgttcg gaaatcgcgt
      901 ggagctcgtc accgccgagt accccggaag cgcatcgctc ggttcgccga gttgggagac
      961 gctcgcgtac gaatggcgcg gatagtcgaa aagttgcgcc gcgctcgccg agcgcaacgt
     1021 tcaacgcccg tcgccgggcg agtcggggca tagcgcggcc tgcaagtgcg agaagcgctc
     1081 ggccagaaaa tcggcggttg ccctgactcg cttcacgctg cgcgcgtccc ggtgcacgcc
     1141 gagccatagc ttgcgcgatg gaggcgcgcc gggtgtctgc agcagacgaa gaccgggcgt
     1201 cgcgtctccg acgaaacgag gcaagcacgt aaagccgacg ccggccgcgg ccatcgtcgc
     1261 catcggctca cgagcgttga gccgcgcaac gactcgcgct cgggcggcaa tcgtcggtaa
     1321 ccagtcgacg tctggcacct tgccgagcga cgcgctcatc gcgatcaagt cgtgcccttc
     1381 accctgtcgc tcgaagtcgg gcgcgccatg gctcgccagg tacgcatccg aggcatacag
     1441 cccaaagccg atcgtggcga cctcgcgctg gacgacgtcg ggctgctcga accgcgaggg
     1501 ccgaatcgcg atgtctgcgt cacgccgctg caagttcagg tgcttgggct cggcgaggag
     1561 ctcgagctcg agctccgggt agcgcgcgag caacggcccg acgatgggcc cgatgacgcg
     1621 ctggatcatc cactcggaag ccgtcacgca cactgggcca gcgacctcgc caccgcgacc
     1681 ggcgagcgct tgcagcgcgg cgagcgcgtc acgctccatt cgctcgacgt gcgtgagcac
     1741 gcgctgccct tcgggggaca agctctgccc agtcgcggtg gcgacgaaga gcggaacgcg
     1801 aagctcgtcc tccaacgcgg cgatgcgccg gctcaccgtc gtttgcgcca cgccgagcgt
     1861 tcgcgccgcc gccgacgcgc tccccgcccg cgccaccgcc agcaaatagc gaagatcgtc
     1921 ccaatccggc acgccggcag cgtagccgaa gcggcgccgg gggtgctgca aaaatgcagg
     1981 gggtcactgc gatgctacgg gctcgacgcg acgccgcgcg cgctgcactc atccgcgcat
     2041 gcaacgacga gagttcctcg ttcacggcgc cactggcgtg ctcatcggct gctcgcccgc
     2101 gctgagcgtc gcacccgcct cgcctcgatc gaagcccgaa gctcgcgctc cgatccgggg
     2161 cgtctgcttc gacttgttca cgttgttcga tccgcgctcc gtggtcgagg tcgcgcgcgc
     2221 catctcaccc gagcgcgcgg tggcgctctg cgaagcgtgg cgggtgcggc agttcgagta
     2281 cgcgccgctc cgtgtcgccg ctggtcgata cgtcgacttt cgcgtcgtga ccaaggacgc
     2341 gctcgagtat gccgcgcgct cacaagcggt tcacctaacc gatgcgcagc gcgaagagct
     2401 ggtggatgct tactcacgcc tcgagccatg gcccgacgcc cgcgccacgc tgctcgcttt
     2461 ccgcagcgcg ggtctgcgct tgagcccgct cgcgaactac tcgccgcgca tgctctctcg
     2521 actgctcgag aactccggct tcgcggagct gttcgacgcg cagatttcca cggacgcggc
     2581 gcgcaccttc aagccggatc cgcgcgcgta cgctctcggc gtcgcgaccc tgaagctccc
     2641 ccgcgagcag attgcgtttg cggcgttcgg cggctgggat gccgcgggcg cgaagtggtt
     2701 cggctacccg acgttctggg tgaaccggct cggcgtgccg cacgaagagc tcgcgcccgg
     2761 gttcgactcg acgggcccct cgctcgcgga gctcgcggcg ttcgtgcgcg actgggcatg
     2821 agctcgatcg gacgcgacga gctcgcggac cgttcaagcg gtcgccggtc tagggggtgt
     2881 cccaagttcc ccgccctgct gcgagccaag atcctgcggc tgcgaagcac ggcctcgtcg
     2941 ctccgctcct cgaaaggctt cagccttcct ccggtgctca ctcctcgggc gcgcttctcg
     3001 ctcgcgtttc
//
LOCUS       DASTKS010000980         7930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_377938, whole genome shotgun sequence.
ACCESSION   DASTKS010000980 DASTKS010000000
VERSION     DASTKS010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377938"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..427)
                     /locus_tag="VFQ35_20675"
     CDS             complement(86..427)
                     /locus_tag="VFQ35_20675"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HET9933136.1"
                     /translation="MAWVAVLLLVVSNIFMTFAWYGHLKNHQHSPLWLVILMSWGIAL
                     FEYCFQVPANRIGAQAGLVTAQLKTVQEVITLIVFSVFAALYLGEPLRWNHLVGFGFV
                     AVGALFVFKPW"
     gene            complement(569..1318)
                     /locus_tag="VFQ35_20680"
     CDS             complement(569..1318)
                     /locus_tag="VFQ35_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933137.1"
                     /translation="MKAVSRSALPREVAEFLQTMPLAYAQSFSVREIEEHARIVARRG
                     TLSAHAEPWRGATSPPYVCVVADDRPGLLSFVTDALLVHGLNLKSAQIYCRPRADGAW
                     EAIDFFCVEPGDASEQLEADELANFAQTLRELIAEEQVVERQSHERDTIPVPKPRPSR
                     VYFDLEALKRNELVLVVDTPDCSGLLFAIASALHGQNARIVASQIRTEDGTARDRFQL
                     ALVDTQPFDGERLCDIQQAVLAAVRSGSKAP"
     gene            complement(1405..2799)
                     /gene="sthA"
                     /locus_tag="VFQ35_20685"
     CDS             complement(1405..2799)
                     /gene="sthA"
                     /locus_tag="VFQ35_20685"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET9933138.1"
                     /translation="MDANHYDLVVLGAGPAGEKGAAQAAYFGKRVLVVEKELEPGGAA
                     VHTGTLPSKTLRETALYLSGYHARELYSVAVELDPTATLQRLMSRKAAIAAAESNAFR
                     ENFVRHGIEYRRGSARFVDAHTVAIRDVEGERQVSADYVLIATGSKPHRPADIDFAST
                     LIHDSDEVLTIDCIPRSLTILGGGVIGCEYACMFAALKTEVTLVDARDEILPFLDAEI
                     VARLKLAMKSAGIRFVQGQRWKGVRATDKDVTTTLGDGSTLTSHQLLFAAGRTGRTDE
                     LNLSAVGITPDSRGYLQVDAHFRTTQAHILAAGDVVGFPALASVSMEQGRVAVCQAFG
                     FDYKRAVAAQMPYGIYTIPEVSSFGETEESCREKKIDYVVGRALFSNNPRGKITGDLD
                     GITKLVVDASTRKLIGVHVIGERASELVHIGQLAVTLGATVDVFIDMVFNYPTLSDSY
                     KYAAYNCLDAMTKR"
     gene            2896..4335
                     /locus_tag="VFQ35_20690"
     CDS             2896..4335
                     /locus_tag="VFQ35_20690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933139.1"
                     /translation="MSLSPGDIIDDKYRIVRLIGEGGMGAVYEGENARIHRRVAIKVL
                     HAEVAANGDAVARFEREAQAAGRIGSDHIVEVLDLGTLPSGARYLVMEFMDGDSLSKR
                     IKQRGKLSPHELYPIARQLLEALAAAHGAGIVHRDLKPDNVFLLASRHGNPDFVKLLD
                     FGISKFSDLHGAVSSMTRTGAVMGTPYYMAPEQAKGSRQLDQRVDLYAVGVVMYEALT
                     GKVPFDAETFNELLFKIVLETPRPIEELAPGMDPNLCAIVRRAMAREPNERFQTADEF
                     RGALDQWAAGGGAPLPDASGRYPQVNPNVRASAPQFNLTPGNWTEVPPGLELAGTRNR
                     KPLYMATAAIVLVVFGAGGFLLISGRESNREAAEAAASASALATSASAVPASSATAAA
                     SAPPVALPSETAASPTGEPSLPMGLPVVGAVAPKPSTQAPLVKPSGPIARPTRRPQAA
                     PPPPPQRPAEPAPTTAKTGKGGRPIRTEL"
     gene            4639..5268
                     /locus_tag="VFQ35_20695"
     CDS             4639..5268
                     /locus_tag="VFQ35_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933140.1"
                     /translation="MHRALAFLSLLAGLAATSNGFAQQAVIKQPGAHTRYGVELEPHF
                     TFQWDGGPDYYADDGVGLGMRVGIPLFHNGPIPQINNNMAISFGFDWVHFGYDRGAAC
                     RRYQGAYCDDWDFSANSLWFPVTLQWNFYVHRRINVFGEVGFAIRHTRYSWLEGCGGP
                     TGYCEYTDSTTRAPYFVFYPGARFLLSDTVSIMVRVGYPHFTAGVSFLL"
     gene            complement(5343..6380)
                     /locus_tag="VFQ35_20700"
     CDS             complement(5343..6380)
                     /locus_tag="VFQ35_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933141.1"
                     /translation="MRRELGMSVALLLMCLGLFVSNPDFLGQSNFINTIRQISMLGIY
                     AVGIAFVIIAGGIDLSIGSVIGLTGVLIAKISSPAAGGLGHSLWIGIPVAILVALVIG
                     LLQGLLITRLKLQPFIVTLGGMLFLRGISQTIVEGGTLSLGGSKLLDLANGGLLRHNG
                     DPLIPYPFLIFLAVVGVATYVLHFTVFGRYVFALGGNRDAAEYSGINVRLVETITYVV
                     SAGLAGVAGVCYAAYIGQMSQQVGIAYEMYAIAAAVLGGCSLRGGEGTVLGVIIGSAV
                     MRVIDNGINMFQLPYVDGEGIHRIWRLNPNWTFVIIGGVILVAVILDQVVHIVQAKRR
                     IRRAALANESG"
     gene            complement(6377..>7930)
                     /locus_tag="VFQ35_20705"
     CDS             complement(6377..>7930)
                     /locus_tag="VFQ35_20705"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9933142.1"
                     /translation="RRDEEVVETASAAPKPLLSMSAITKRFPGVVALSDVSLSLAAGE
                     VLALMGENGAGKSTLMKILGGAQPPDGGTVAIDGANVALGSVRDAKGLGIALIHQELM
                     LVPTLDIAANIFLGNEQGRKFWLGPLDRKPMRDAAAKLLERVGLGVSPDALVSTLTAG
                     QMQMVEIAKALALNARIIIMDEPTSSLTSGESEHLFRIIRQLKADGIGIIYISHRMEE
                     VLKLADRITVLRDGRYVGDLLRAEATHDKVVALMVGRELSGNYFPEHGADADAEPVLE
                     VKDLLVPGSPAGVSFTALRGEILGFAGLVGAGRTELMQTLFGVTPALAGSVTLEGKAF
                     LPRSPREAIDRGVYLAPEDRKRHGVVLPMTIAENTSLPSVGSYNRFGFLDRKRERRVA
                     EAEVKRLRTKASSIEQRVVNLSGGNQQKVVLAKWLAMSPKVLILDEPTRGIDVGAKAE
                     IYRHMADLAKRGITVVMVSSDMEEIIGMSDRVVVMHERRIRGVLAREQLSQQRIAELM
                     TGKSDGVAA"
BASE COUNT         1443 a         2581 c         2546 g         1360 t
ORIGIN      
        1 cgaggaactc cgataaagtc atccgagtga cgccgctacc gtttacgtag ggccagaaga
       61 gacctttcgc aacgggcgca ggacctcacc aaggtttgaa cacgaacagc gcgcccaccg
      121 cgacgaaccc aaacccgacc aagtggttcc aacgcagcgg ctcgccgagg tacagcgccg
      181 cgaagacact gaacacgatg agcgtgatca cctcttgcac ggtcttcaat tgggccgtca
      241 cgaggccggc ttgcgcgccg attcggttcg ctggcacctg aaagcagtac tcgaagagcg
      301 cgatgcccca gctcatcaaa atcaccaacc agagcggcga atgctggtga ttcttcaggt
      361 gcccgtacca ggcgaaggtc atgaagatgt tcgagacgac gaggagcaga actgcaaccc
      421 aagccatgcg cgaaggtttg ttgcggaaac gccgctcctt ggcaagcgtg tggcgaagtt
      481 gcgagcgttc cgcccgaagt tagggggggc gagctctgct gcgacagttt tgcccggccg
      541 agccggagcc tggtgggaag acgtcagctc agggcgcctt gctgccggag cgcaccgccg
      601 ccaaaacggc ttgctggatg tcgcacaagc gctctccgtc gaacggctga gtgtcgacga
      661 gcgcgagctg gaaccggtcg cgcgcggtgc cgtcttcggt gcggatttgt gacgcgacga
      721 tgcgcgcgtt ttgtccatgc aacgcgctcg cgatggcgaa caagagaccc gagcagtcgg
      781 gcgtgtcgac caccagcacg agctcgttgc gcttgagcgc ctcgaggtcg aagtagacgc
      841 gcgaaggtcg tggcttgggg accgggatcg tatcgcgctc gtgcgactgc cgctcgacga
      901 cctgttcttc ggcgatgagc tcgcgcaaag tttgagcgaa gttggcgagc tcgtctgcct
      961 cgagctgctc actcgcgtcc cccggctcca cgcagaagaa gtctatcgcc tcccacgcgc
     1021 cgtccgcgcg aggccggcag tagatctgcg cgctcttgag gttcaagccg tggacgagca
     1081 acgcgtcggt gacgaacgag aggagccccg ggcgatcatc agcgacgacg caaacgtaag
     1141 gaggtgacgt cgcaccacgc caaggctccg cgtgcgccga cagcgtgccg cgacgagcga
     1201 cgatgcgggc gtgctcctcg atttcgcgca cgctgaagct ctgcgcgtac gcgaggggca
     1261 tcgtttgaag gaattcggcg acctcgcgcg gcagggcgct tctgctcaca gccttcattc
     1321 cggtcagaag cgtacggata gccgtggcgc tgttccagga ggctttcgcc ctcttttccg
     1381 cgacgtgtcc gggtttcccg cgggtcagcg cttggtcatg gcatcgaggc agttgtaagc
     1441 ggcgtacttg taagaatccg acaaggtcgg atagttgaag accatgtcga tgaacacgtc
     1501 gaccgtggcg ccgagcgtca ccgcgagctg gccgatgtga acgagctcgc tcgcgcgctc
     1561 accgatcacg tgaacgccga tcaacttgcg cgtgctggcg tctacgacga gctttgtgat
     1621 gccgtcgagg tcgccggtga tcttgccgcg gggattgttc gagaacagcg cgcgaccaac
     1681 cacgtagtcg atcttcttct cgcgacaact ctcctccgtt tcgccgaacg agctcacctc
     1741 ggggatggtg tagataccgt aaggcatctg cgccgcgacc gcgcgcttgt agtcgaagcc
     1801 gaacgcttgg caaacggcga cgcgtccctg ctccatcgaa accgacgcga gcgcggggaa
     1861 accaacgacg tctcccgcgg cgaggatgtg cgcctgcgtc gttcgaaagt gagcatccac
     1921 ctgcaggtat ccgcggctgt ccggcgttat gccgaccgcg ctcaggttga gctcgtcggt
     1981 gcgaccggtg cgaccggcgg cgaacaagag ctggtggctc gtgagcgtcg agccgtcgcc
     2041 gagcgtggtc gtcacgtctt tgtccgtggc gcgcactccc ttccagcgtt gcccctggac
     2101 gaagcggatg ccagcgctct tcatcgcgag cttcaagcgc gcgactatct ctgcgtcgag
     2161 gaacgggagg atctcgtcac gcgcgtcgac gagcgtcacc tccgtcttca gcgcggcgaa
     2221 catgcaagcg tactcgcaac cgatcacgcc gccgcccaga atcgtcagag atctcgggat
     2281 gcagtcgatg gtcagcacct cgtcgctgtc gtgaatcagc gtgctcgcga aatcgatgtc
     2341 cgccgggcga tggggcttcg accccgtcgc gatcaacaca tagtcggcgc tgacctggcg
     2401 ttctccttcg acgtcgcgaa tggcgacggt atgcgcgtcg acgaagcgcg cgctgccccg
     2461 ccgatactcg atcccgtggc gcacgaagtt ttctcggaag gcgttcgact cggcggcggc
     2521 gatggcggcc tttcgtgaca tcaagcgctg gagcgtcgcg gtgggatcga gctccaccgc
     2581 tacgctgtag agctcgcgcg cgtggtagcc ggacaggtac agcgccgtct cgcggagggt
     2641 cttgctcggc agcgtcccgg tgtgcacggc ggcgccgccg ggctcgagct ccttctccac
     2701 gaccagcacg cgctttccga agtaggcggc ctgcgcggcg cccttctcgc cggctggacc
     2761 cgcgccaaga acgaccaaat cgtagtggtt agcgtccatt ctcgagcagc atgctacatc
     2821 gtccgaccac ggcccaatac tcggggcgct cggccgagcc agcggcgtgg cctttgtgct
     2881 acggatcgga gctccatgag cctgagcccc ggcgacatca tcgacgataa gtaccgcatc
     2941 gtgcgactca tcggtgaggg cggcatgggc gccgtctacg agggagagaa cgcgcgaatc
     3001 caccgccgcg tcgcgatcaa ggtgctgcac gcggaagtgg cagcgaacgg cgacgcggtc
     3061 gcgcggttcg agcgcgaagc gcaagcggcg gggcgaattg gttccgacca catcgtcgag
     3121 gtgctcgacc tcggcactct cccgagcggc gcccgctacc tcgtgatgga gttcatggac
     3181 ggcgacagcc tgagcaagcg catcaagcag cgcggcaagc tatcgcccca cgagctctac
     3241 ccgatcgctc ggcagctgct cgaggcgctg gccgccgcgc acggtgccgg catcgtccat
     3301 cgcgacctga agcccgacaa cgtctttttg ttggcgagcc gccacggcaa ccccgacttc
     3361 gtcaaactgc tcgatttcgg catctcgaag ttttcggatt tgcacggcgc ggtgagcagc
     3421 atgacgcgca ctggggccgt aatgggcacg ccctattaca tggcgccgga gcaggccaag
     3481 ggctcacgac agctcgacca acgcgtcgat ctttatgcgg tcggcgtcgt catgtacgag
     3541 gcgctcacgg gcaaagtgcc tttcgacgcc gagaccttca acgagctcct cttcaagatc
     3601 gtgctcgaaa cgcctcgccc catcgaagag ctcgcgcccg gaatggaccc gaatttgtgc
     3661 gcgatcgtcc gccgcgcgat ggcgcgcgag cccaacgagc gctttcagac cgcagacgag
     3721 tttcgcggcg cgctcgatca atgggcagcg ggaggaggag ctccgctgcc agacgcgagc
     3781 ggacgctacc ctcaggtgaa tccgaacgtg cgggccagcg cgccgcaatt caacctgacg
     3841 cctggaaact ggacggaggt cccgccaggt ctcgagttgg cgggaacgcg caatcgaaag
     3901 cctctttaca tggcgacggc cgcgatcgtg ctggtcgttt tcggcgccgg cgggttcttg
     3961 ctcatcagtg ggcgcgagtc caaccgcgag gccgccgagg cggctgcgtc tgcctcggcg
     4021 ctcgcaacga gcgcgtcggc cgtccctgca tcgagtgcca ccgctgccgc gagtgcgccc
     4081 cctgtcgctc tcccgagcga gaccgccgca tcgccgaccg gcgagcccag cctgcccatg
     4141 gggctgcccg tcgttggggc cgtcgctcca aagccgagca cgcaggcgcc tctcgtgaag
     4201 ccctcagggc cgatcgcccg cccgacgcga cgcccgcaag cggcgccacc acccccaccg
     4261 cagcggcccg ccgagccggc gccaacgacc gcaaaaaccg gcaaaggcgg gcgcccgatc
     4321 cgcactgagc tctgagaagt tagctcgcgc gtacgacttc gagagtgcgc cgcgaagtga
     4381 cattcgtaag cgctagggcc tgcggccggc gccatgctgg aagagttctt gcttgtgcgc
     4441 tcggaattgg tttctcgtct cggtgccatg gctcgcctgc tcgatgtcac cgacccgcaa
     4501 cctcaagggc tgcgggcgga atccgtgtgc gccgagacgt ggagaaatcg agggggacaa
     4561 ccggaaatag gccggcgtga acgcgcggta aagaaattcg ccgccgttac gccattcact
     4621 gaaggacgga actgatccat gcaccgagcg ctcgcatttc tgtcactcct cgccggtctc
     4681 gcagcgactt cgaacgggtt tgctcagcaa gccgtgatca aacagcccgg cgcccacacc
     4741 cgctacggcg tcgagctcga accgcatttc accttccagt gggatggcgg gcccgactac
     4801 tacgcggacg acggcgtggg gctcgggatg cgcgtcggga tcccgctatt tcacaacggg
     4861 ccgatcccgc agatcaacaa caacatggcg atctcgtttg gcttcgactg ggtgcacttc
     4921 ggctacgacc gcggtgcggc ctgtcggcgc taccagggcg cctactgcga cgactgggat
     4981 ttcagcgcga attcgctgtg gttcccggtc acgctgcaat ggaacttcta cgtccaccga
     5041 cgcatcaacg tgttcggcga ggtcggcttc gcgattcgac acacgcgcta ctcgtggctc
     5101 gagggctgcg gcggtccgac cggctactgc gagtacacgg acagcacgac gcgggcgccg
     5161 tacttcgtgt tctacccggg cgcgcgcttc ttgctgagcg acacggtgag catcatggtg
     5221 cgcgtcggtt acccgcactt tacggcgggc gtctcgttcc tactctgagg gccaaggcga
     5281 agctcagccg ctcgtttcgg gctgggcttc gcttggtcga accgggcaag cagggcgtcg
     5341 gctcagccgc tctcgttcgc gagggcggcg cggcggatgc ggcgcttggc ctgaacgatg
     5401 tgcacgacct ggtcgaggat cacggcgacg aggatgacgc cgccgatgat cacgaaggtc
     5461 cagttcgggt ttaggcgcca aatgcggtgg atgccttcgc catcgacata cggcagttgg
     5521 aacatattga tgccgttgtc gatgacgcgc atgaccgccg agccgatgat cacgccgagc
     5581 acggtgcctt cgccgccgcg cagtgagcaa ccgccgagta cggcagcggc gatcgcatac
     5641 atctcgtacg cgatgcccac ttgttgggac atctgcccga tgtaagccgc gtagcaaaca
     5701 ccagcgacgc ccgcgaggcc cgccgaaacg acgtacgtga tcgtctcgac caggcgcacg
     5761 ttgatgccag agtattcagc tgcgtcccgg ttgccgccga gggcaaacac gtagcgcccg
     5821 aacacggtga agtgcagcac gtaagtagcg acgccgacca cggcgagaaa aatcaagaac
     5881 ggatacggga tgagcggatc gccgttgtgc cggagcaacc caccgttcgc gagatcgagc
     5941 agcttggagc caccgaggct cagcgtgccg ccttcgacga tggtctggga aatgccgcgc
     6001 aagaagagca tgccgcccag cgtgacgatg aacggctgca gcttgagtcg cgtgatgagc
     6061 aacccctgca gcaggccgat cacgagcgcg accaggatgg cgaccgggat accgatccac
     6121 agagagtgac caaggccgcc cgcggccggc gacgagatct tcgcgatcaa aacgccggtg
     6181 agaccgatca ccgagccaat cgacagatcg atgccgccgg cgatgatgac gaaggcgatg
     6241 ccgacggcat aaatgccgag catcgagatc tgtcggatgg tgttgatgaa gttcgactgc
     6301 cccagaaagt cggggttgga aacgaagagg ccgaggcaca tcaagagcag cgccacgctc
     6361 atcccgagct cgcgcctcat gccgctaccc cgtcgctctt tccggtcatg agctctgcga
     6421 tgcgctgttg gctcagctgt tcacgagcga gcacgccgcg gatacgccgc tcgtgcatga
     6481 cgaccacgcg gtcgctcatc ccgatgatct cctccatgtc cgagctcacc atgacgacgg
     6541 tgatgcctcg cttcgcgagg tccgccatgt gtcgataaat ttcggctttg gcgccgacgt
     6601 cgatgccgcg cgtgggctcg tccaaaatca agactttggg gctcatggcg agccatttgg
     6661 cgagcaccac cttctgctgg ttaccacccg aaaggttgac cacgcgctgc tcgatgctcg
     6721 aagccttcgt gcgcaggcgc ttgacctcgg cttcggccac gcggcgctct cgcttgcgat
     6781 ccagaaaacc gaagcgattg tagctcccga cgctcgggag cgacgtattc tcggcgatgg
     6841 tcatcggcag caccacgccg tgtcgcttgc gatcttccgg cgcaaggtac acgccgcggt
     6901 caatcgcttc tcgcggcgag cgtggcaaga acgctttccc ttcgagcgtg acgctacctg
     6961 cgagcgcggg cgtcacaccg aacagcgttt gcatcagctc ggtgcgaccg gcaccgacga
     7021 ggcccgcaaa gcccagaatc tccccacgca gcgccgtaaa ggacacgccc gcgggtgagc
     7081 ccgggacaag aaggtccttc acttcgagca cgggctccgc gtccgcgtcc gcgccatgct
     7141 cggggaagta gttgccgctg agttcgcgcc cgaccatcag cgcgacgacc ttgtcgtgag
     7201 tggcttcggc gcgcagaagg tcgccgacgt agcgcccgtc gcgcagcacc gtgatgcggt
     7261 cggcgagctt caggacctct tccatgcggt gcgagatgta aatgatgccg ataccgtcgg
     7321 ccttgagctg ccgaatgatg cgaaacaagt gctccgactc gccgctcgtg agcgacgacg
     7381 tcggctcgtc catgatgatg atgcgcgcgt tcaatgcgag cgccttcgcg atctcgacca
     7441 tctgcatttg gccagcggtc agcgtcgaaa cgagcgcatc cggcgaaacg cctagtccga
     7501 cgcgctcgag taactttgcc gcggcgtcgc gcattggctt tcggtcgagc ggacccaacc
     7561 agaactttcg cccctgctcg ttgccgagaa aaatgtttgc ggcgatgtcc agcgtcggca
     7621 ccagcatcag ctcttgatga atgagcgcga tgccgagccc cttcgcgtcg cgcacggagc
     7681 cgagcgcgac gttcgctccg tcgatggcga cggtgccgcc gtcgggcggc tgcgcaccgc
     7741 ccaggatctt catcaacgtg ctctttccgg cgccgttttc gcccatcagc gcgagcacct
     7801 cccccgcggc gagcgacagc gacacgtcgg acagcgccac cacgcccgga aagcgcttgg
     7861 tgatcgcgct catcgagagc agcggcttcg gcgcagcgga cgctgtttcc actacttctt
     7921 catctcgacg
//
LOCUS       DASTKS010000981         9189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1718842, whole genome shotgun sequence.
ACCESSION   DASTKS010000981 DASTKS010000000
VERSION     DASTKS010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718842"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..912)
                     /locus_tag="VFQ35_20710"
     CDS             complement(181..912)
                     /locus_tag="VFQ35_20710"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9933143.1"
                     /translation="MPIERVQTTFFQDPLWAESLADLVLPEQLERARAARLFRVWAPA
                     CGMGEELYSVSVLLAQALPDGDDWSIFLTGTDTDDARLNRARRAAYAEGALRAFTREE
                     RACYFRYNPNTRRYGLRERYIRNAHFGYRTLVDVTSAAPTPGSFELILCPDSVSALPV
                     SEQRAVFGYFTQVLSERGVVVARKQIEGVETMHRAFHAGLVAHHKGSVSQPVSTLPSS
                     ERPTARPPAPSSDWSRALSNERSVA"
     gene            complement(1048..1854)
                     /gene="tsaA"
                     /locus_tag="VFQ35_20715"
     CDS             complement(1048..1854)
                     /gene="tsaA"
                     /locus_tag="VFQ35_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET9933144.1"
                     /translation="MSEIPDAPPPLPTLTLTPIGVVHSPFQERMQAPRQPRAALGVEG
                     SIELFPATGIDHALEDLDTFRFIWVLFWFHKNDGFRPKVLPPRSQERRGVFATRSPYR
                     PNPIGLSVVELVAVEGRRLRIRNLDILDGTPVLDIKPYVPYTDAIPEANNGWLDDARA
                     PRDPLPEYSVSVSPLASEQFAFIEAHEPGVRAGVEEVLRLGPEPHPYRRIKRVDDGFV
                     LAHKAWRVAFECAGQHIVVRAVKSGYRPSELFGSSGAELEHHRQFVDRFG"
     gene            1942..2604
                     /locus_tag="VFQ35_20720"
     CDS             1942..2604
                     /locus_tag="VFQ35_20720"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9933145.1"
                     /translation="MTFAVVTGSNRGIGLELCRGLKARGYQVLAACRTRSKELDALSV
                     EIVDGVEVSTEAGVRKLKEAVGTRSIDVLVNNAGILEHEALPDIDFAAIARQFEVNAM
                     GPLRVTAALVPRLGRGSKVALITSRMGSIADNGSGAYYGYRMSKAALNAAGVSLARDL
                     STRGIAVALLHPGYVRTEMTGNSGGIEPKDAAAGLLARIDALTLETSGGFWHQNGEPL
                     PW"
     gene            complement(2606..3517)
                     /locus_tag="VFQ35_20725"
     CDS             complement(2606..3517)
                     /locus_tag="VFQ35_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HET9933146.1"
                     /translation="MSATPSPAARLRALLAEPGILLMPCCYDALSARLIEEAGFPLSF
                     MSGFSVSAARLGMPDTGLISYGEMVDQGRSICAAVQIPVIGDGDTGYGNAVNVKRTVE
                     GYRQAGFAGIMIEDQVSPKRCGHTLGKEVVARRDALTRLRAAVDARDEGADIVIVART
                     DARATHGFQEALYRAEAFADAGADVIFLEAPVDRDEMKEFCHRVAKPTLANMLEGGRT
                     PWVPPNELEELGYKIAAYPVTLLSAAVAAMRRVLDEVQRGMPATGLLSFAELRDVVGF
                     DAYDAVARRYRGESEVPSTPRPPPHSG"
     gene            3692..4402
                     /locus_tag="VFQ35_20730"
     CDS             3692..4402
                     /locus_tag="VFQ35_20730"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9933147.1"
                     /translation="MAKLEGRHILLTGISRGVGLETARLFLAEGAQLFGVGKDANRLE
                     LVRKELDPTGRRLAVLAADLASPDAARKIADAVSRHWPKLDILLNNAGIQIDAGARGF
                     TDGTEAALTQSMEVNLMAPYRLALALLPMLKAGVEPRIINVSSGAGTFAAMKDERIAS
                     YRLSKWALNGFTLLLSAELAGQVAVNAFDPGWVKTDLGGPNAPGSPTESAQGALAIAT
                     LPFSESGKFWKDGKEIPF"
     gene            4430..5182
                     /locus_tag="VFQ35_20735"
     CDS             4430..5182
                     /locus_tag="VFQ35_20735"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET9933148.1"
                     /translation="MTKLSVNVNKIATLRNTRTLGIPNLVRLSAIALEAGADGITIHP
                     RPDQRHIRKEDVPELAALLRGYPRAEFNIEGNPFHGLVEHCVASRPTQATLVPDAREA
                     FTSNHGWNLIELGSVERNGLRRSIESLHEVGCRVSLFIDPVPEMMPLARELGADRVEL
                     YTEPYAAAALAGQAEASLKKYATAADAARRSGLGVNAGHDLSLLNLSPFLQALGAVDE
                     VSIGHALIADALELGLSETVRRYKALCVRTVH"
     gene            complement(5188..7032)
                     /locus_tag="VFQ35_20740"
     CDS             complement(5188..7032)
                     /locus_tag="VFQ35_20740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET9933149.1"
                     /translation="MSRLLSGCVREGRSRGEGAIVGAFSGPVGLIPALAPGLRRFNVS
                     VPEPESLARRVARLNDVAQALRGGDLQRRAEVDRGLRSLRDEYRRNPNGFSTDLVGML
                     KSVAQSLAEPPSRSALERVLKDTFGYDSFRPGQLPIIESVLSGKDCVGIMPTGAGKSL
                     TFQLPARVLGGTTLVISPLIALMKDQVDGVNEVGMRATFLNSSLPPDERRERINRLRR
                     GEFELVYAAPEGLEASAGRTILDLDIGLIAVDEAHCISQWGHDFRPAYRNLMGLKRRF
                     PNTPVLALTATATDDVSKDIVAQLGMRSPNVFRGSFLRKNLRVSAIPKSELPRSTKHA
                     IAQIVASRRGESGIVYCLSRRSTEATAEHLLSQGLSAAHYHAGLEPAERARVQDAFRS
                     GSVDVVVATIAFGMGIDKPDVRFVLHHDLPRSIEGYYQEIGRAGRDGLPSDCVLFYSF
                     ADVKAYERMAEQGDEEASARLVAQARETFRLVEAEGCRHQNIVGYFHEEVEPCGTACD
                     RCGKLPARRLVEQRPARGQKAADMRSSEPREVLSADAALFDKLKQERRRIADELKVPA
                     YVVFTDATLLEMAARRPSSEAELLNVSGVGLRKLERYGERFLALLREA"
     gene            7371..9131
                     /locus_tag="VFQ35_20745"
     CDS             7371..9131
                     /locus_tag="VFQ35_20745"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9933150.1"
                     /translation="MKAGSVLSGKYRLVSILGQGGMGSVWRAEHLALRAPVAVKLLAP
                     AFEARPETWARFHREAQSAANLRSPHVVQILDHGVDQETQTPFIAMELMEGESLAERL
                     SRVGRLSPEDTLKVITHTARALTRAHDAGILHRDLKPGNLFIVHNDDDEIVKVLDFGL
                     AKWRVDPEGQHAAATVTGQLLGTPYYMSPEQVEASNQVDYRSDLWSLGVIAYECLVGA
                     RPFEGDSLMAIAFKICERKLSPLPETLPLPRALNAWFERALAKSPAARFQSARELAED
                     LRRVFGAPTSHVGADSTAETGAAAAVHRPAQSPPGNGGAEADARPSQGTVDMGRPSFV
                     PPQAPERFDAGEPAAPPPAPGFEETVEAVVDYAAALGKRAEVAPSNSSSPNDAGEAAS
                     VEPPPISDQSVLPVARSTNRAVRASDKRTPRASVVLVGVAVAALAGVAALALRGPRGT
                     RSPDAAAANVVNNRERVDERALPSRLVAPTPGVRDSAATSTTALPSQPSAGPVVAPES
                     APPLAASAPPLVAVASASPALPAPLAKPSSAAGTAQPNAPAGALTPTRENTPKTSAAP
                     APKAKPRANVGQSKVWLERE"
BASE COUNT         1629 a         3065 c         3004 g         1491 t
ORIGIN      
        1 cctggctacg cgcgcttcac cctgaagtcg ggccgccaca tggaagcgat cgtacgaatt
       61 ttgacgcctt gaaatgcgtc gcgtctcgcg gcgttccgag agagccgaag aaaggtgtgg
      121 gctagagggg gcccggggat cgggcgatga cagggcgcgt ctcgcgtcgt gcgctagtcg
      181 ctaggccacg gagcgctcgt tcgagagagc gcgcgaccaa tcgctgctcg gggcgggagg
      241 acgcgcggtg gggcgctcgc tgctcggcag agtcgagacc ggttgcgaga cactaccctt
      301 gtgatgcgca acgagcccgg catgaaaggc ccgatgcatc gtctcgacgc cctcgatctg
      361 cttgcgcgca acgacgaccc cacgctcgga gagtacctgc gtgaaatagc cgaacacggc
      421 gcgctgttcg gagacgggca acgccgagac gctgtccgga cacaagatga gctcgaagct
      481 gccaggagtc ggggccgcgc tcgttacgtc aacgagggtg cgatacccaa agtgggcgtt
      541 gcggatgtag cgttcgcgaa gcccgtagcg gcgggtgttc gggttgtagc gaaagtagca
      601 ggcgcgttcc tcgcgagtga aggcgcgcag cgctccttcc gcgtacgcgg cccggcgcgc
      661 gcgattcaaa cgagcatcgt ccgtgtcggt gcccgtgaga aagatgctcc agtcatcacc
      721 gtcgggcagg gcttgcgcga gcagcaccga cacgctgtag agctcctcac ccatcccaca
      781 cgcaggcgcc cacacgcgaa acagccgcgc cgcgcgcgcc cgctcgagtt gctcgggcaa
      841 aaccaggtcg gcaaggctct cggcccaaag tggatcttgg aagaaggtcg tctgaacgcg
      901 ctcgataggc atggcttctg cgggccgttt agcaactgcc gagccaacgt gagtgcgcgg
      961 tttttctcgg aatttaagcg agggcgggga ggcgcgagtg tcgaactttc gacgcccgag
     1021 gcgcgttttt tgacgcttcc cgccacgtca gccgaagcgg tcgacgaatt gccggtgatg
     1081 ctcgagctcc gcgccgctcg agccgaacag ctcgctcgga cggtacccgc tcttcaccgc
     1141 gcgtacgacg atgtgttgcc cagcgcactc gaatgcgaca cgccaggcct tgtgggccag
     1201 cacgaaaccg tcgtcgaccc gcttgatgcg acgatagggg tgcggctccg gaccgaggcg
     1261 caacacctct tcgacccccg ctcgcactcc gggctcgtgg gcttcgatga acgcaaactg
     1321 ctcgctcgcg agcggcgaga cgctcaccga atactcgggc agggggtcgc gcggcgcgcg
     1381 cgcgtcgtcg agccaaccgt tgttcgcctc gggaatggcg tcggtgtacg gaacgtaagg
     1441 cttgatgtcg agaacgggcg tgccgtccag aatatccaag ttgcgaattc gaagccgtcg
     1501 cccttcgact gcgacgagct cgacgacgga gaggccgatc gggttcggcc gatagggtga
     1561 gcgcgtcgcg aagacacccc gtcgctcctg gctgcgcggc ggcagaacct tggggcggaa
     1621 gccgtcgttc ttgtgaaacc agaagagcac ccagatgaag cggaaggtat ccagatcctc
     1681 gagcgcgtga tcgatgccgg tcgcgggaaa tagttcgatg ctgccttcga cgccgagcgc
     1741 agcgcgcggc tgtcgaggag cctgcatgcg ctcttggaac ggcgagtgca cgacgccgat
     1801 gggcgtcaac gtcagggtcg gcagcggtgg cggagcgtcg gggatttccg acatggcgcg
     1861 cagatttgcg cgggccgcga gcgctgtcga gcgattcgga ggaatcttgt tcctgcgcgc
     1921 tgggtttgtc atgttccggc tatgacgttc gcagtcgtaa ccggatcgaa ccgagggatc
     1981 gggctcgagc tctgtcgagg gctgaaggcg cgagggtacc aagtgctcgc agcgtgccga
     2041 actcgctcca aagagctcga cgcgctcagt gtggagatcg tcgatggggt cgaggtttcg
     2101 accgaagcgg gggtgcgaaa gttgaaggaa gccgtcggga cgcgctcgat cgacgtcctc
     2161 gtcaacaacg caggcatcct cgagcacgaa gcgctgcccg acatcgactt cgccgcgatc
     2221 gcgaggcagt tcgaggtgaa cgcaatgggt ccgctgcgcg tcaccgccgc gctcgtcccc
     2281 cggctggggc gcggctccaa agtcgcgctc atcacgagcc gcatgggctc gatcgccgac
     2341 aacggctccg gcgcatacta cgggtaccgc atgtcgaagg ccgcgctgaa cgcggccggg
     2401 gtctcgctcg cgcgcgattt gagtacccgc ggcatcgcgg tcgcgctgct gcacccaggc
     2461 tacgtgcgca ccgagatgac cggcaacagc ggcggcatcg agccgaagga cgccgccgca
     2521 gggctgctcg cgcgcatcga cgccctcacg ctggaaacga gcggcgggtt ttggcaccag
     2581 aacggcgaac cgctgccctg gtagctcacc cagagtgagg cggcggccgc ggcgtactcg
     2641 gcacctccga ttcgccgcga tagcgccgcg ccacggcgtc gtaagcgtcg aaccccacca
     2701 cgtcccggag ctccgcgaag gagagcaaac cggttgccgg catgccgcgc tgcacttcgt
     2761 cgagcacgcg ccgcatcgcc gcgacggccg cgctcagaag cgtgaccgga tacgcggcaa
     2821 tcttgtagcc gagctcctcg agctcgttcg gtggaaccca cggcgtgcgc cccccttcca
     2881 acatgttggc gagggtcggc ttcgcgacgc gatggcagaa ctccttcatc tcgtcgcgat
     2941 cgaccggcgc ctccaagaag atcacgtccg cgccggcatc cgcgaacgcc tcggcgcggt
     3001 agagcgcctc ctgaaagcca tgcgttgccc gcgcgtcggt gcgcgccacg ataacgatat
     3061 cggcgccttc gtctcgcgca tcgaccgcag cgcgtaaccg cgtgagagcg tcgcgccgcg
     3121 caacgacctc tttacctagc gtgtgcccgc accgcttggg cgacacctga tcttcaatca
     3181 tgatccccgc gaagcccgcc tgccggtagc cctcgacggt gcgcttcacg ttcacggcgt
     3241 tgccgtagcc ggtgtcgcca tccccgatca cggggatctg cacggcggcg caaatcgaac
     3301 gcccttggtc gaccatttcg ccgtacgaaa tgagccccgt gtccggcatt ccaagccttg
     3361 ccgccgacac gctaaaacca ctcatgaagc tcaacgggaa gcccgcctcc tcgatgagtc
     3421 gcgccgaaag agcgtcgtaa cagcacggca tgagcaagat gccgggctcg gctagcaggg
     3481 ctcgcaagcg agcggcggga gacggcgttg cggacatgaa gagactttcg cccgatgcgc
     3541 cgcaaattgc gagcgcgcgg tagtgacccc tcggtcctat ccgcccgatt cgctctccaa
     3601 atgggcgatt gagctcaaga tccggcggag tccgtcacgt ttgcgcgaat ccggtgcgtc
     3661 ttggggaaac tccctgttaa aggggtgtgt catggccaaa ctcgagggtc gacacatctt
     3721 attgaccggc atttcacgcg gcgtgggcct tgagacggcg cgcttgtttc tcgccgaagg
     3781 cgcgcaactc ttcggggtcg gcaaggacgc aaatcgcctc gaactcgtcc gaaaagagct
     3841 cgacccaacc ggccgtcgat tggccgtgct cgcggccgac ctggcctctc ccgacgcggc
     3901 ccgcaaaatc gcggacgcgg tctctcgaca ctggccgaag ctcgatattt tgctgaacaa
     3961 cgcggggatt cagatcgacg cgggcgcacg cgggttcacc gacggaacgg aggccgccct
     4021 cacccagtcg atggaagtga acttgatggc tccttatcga ctcgcgctcg cgctcttgcc
     4081 gatgctcaag gccggcgtcg agccacgcat catcaacgtg agctcgggcg cggggacgtt
     4141 cgcggcgatg aaagacgagc gcatcgcgag ctaccgcctg agcaaatggg cactgaacgg
     4201 ctttaccctc cttctctcgg ctgagctcgc aggccaagtc gccgtgaacg cgttcgaccc
     4261 gggctgggtc aagacggatc tcgggggccc gaacgcgccc ggcagcccca ccgaatcggc
     4321 ccagggagcg ctcgcgatcg ccaccctccc cttctccgag agcgggaagt tctggaagga
     4381 cggcaaagag atcccgttct aggcggaggc gtgtcacggt agggcccgca tgacgaagct
     4441 ctccgtgaac gtgaacaaga tcgcgaccct ccggaatacg cgtacgctcg ggatcccgaa
     4501 cttggttcgg ctctcggcaa tcgcgctcga ggcaggcgcg gacggcatca cgattcaccc
     4561 gcgccccgac cagcgtcaca tccgaaagga ggacgttccg gagctcgcgg ccctgctacg
     4621 aggctacccg cgcgctgaat tcaacatcga gggcaatccg tttcatgggc tcgtggaaca
     4681 ttgcgtcgcg tcgcgcccca cgcaggcaac gctcgtaccc gacgcgcgcg aggcgttcac
     4741 cagcaatcac ggctggaact taatcgagct cggttcggtg gagcgcaatg ggctgcgccg
     4801 ttcgatcgag tcactgcacg aagtcggctg ccgagtgagc ctgttcatcg acccggtgcc
     4861 ggaaatgatg ccgctcgcgc gagagctcgg cgccgaccgg gtcgagctct acaccgagcc
     4921 gtacgcggcc gcggccctcg ccggtcaagc cgaggcgtcg ctgaaaaagt atgcgaccgc
     4981 cgcggacgcg gcgcgccgga gcggcctggg cgtgaacgcc gggcacgatc tgagcctctt
     5041 gaacctgtca ccgttcctgc aagcgttggg cgcggtcgac gaagtctcta tcggccacgc
     5101 gctcatcgcc gacgcgctcg aactcggtct ttccgagacc gtccggcgct acaaagcgct
     5161 gtgcgttcgc accgtacact gagcacttca cgcttcgcgc aagagcgcga ggaagcgctc
     5221 gccgtaccgt tcgagcttcc gaaggccgac cccgctcacg ttgaggagct ccgcttccga
     5281 gctgggtcga cgagccgcca tttcgagcag ggtcgcgtcg gtgaatacga cgtaggccgg
     5341 tactttgagc tcgtcggcga tgcgtcggcg ctcttgcttg agcttgtcga acaacgccgc
     5401 gtcggccgag agcacctcgc gcggctccga cgaacgcatg tcggcagctt tttggccccg
     5461 cgccggacgc tgctcgacga gccgtcgcgc gggaagcttg ccgcagcggt cacaggcagt
     5521 gccgcacggt tcgacctctt cgtgaaagta gccgacgatg ttttgatggc ggcagccctc
     5581 ggcttcgacg agccgaaacg tctcgcgcgc ttgagcaacg agccgcgccg acgcttcttc
     5641 gtcgccctgc tcggccatgc gctcgtacgc cttcacgtcg gcgaacgagt agaagagcac
     5701 gcagtcgctc ggcaaaccgt cgcgtccagc gcggccgatc tcctggtaat agccctcgat
     5761 cgagcgcggc aaatcgtggt gcaatacgaa gcgcacgtcg ggcttgtcga tacccatccc
     5821 gaaggcgatc gtcgccacga cgacgtcgac gctaccggag cgaaaagcgt cttgcacgcg
     5881 cgcacgctcg gccggctcga gaccagcatg gtaatgcgcg gcgctcaagc cttggcttag
     5941 cagatgctcg gcggtggcct cggtgctccg gcgcgacaag caatacacga tgccgctctc
     6001 tccgcggcgc gacgcgacga tctgcgcgat cgcatgcttc gtcgaacgcg gcagctcgct
     6061 cttcggaatc gccgaaacgc gtaggttctt tcgcaagaag gagccgcgga acacgttggg
     6121 cgagcgcatc ccgagctgcg ccacgatgtc cttcgacacg tcgtcggtcg ccgtcgccgt
     6181 gagcgccaag acgggcgtat tgggaaagcg ccgcttcagc cccatcaagt tccgatacgc
     6241 cggacggaag tcgtgccccc actgactgat gcagtgcgct tcgtccaccg cgatgagccc
     6301 gatgtcgaga tccaaaatcg tgcgaccagc ggatgcttcg agcccctcgg gcgcggcata
     6361 gacgagctcg aactcgccgc gccgcaagcg attgatgcgc tcgcgccgct catccggggg
     6421 caacgaggag ttcaggaacg tggcgcgcat cccgacctcg ttcacgccat ctacctgatc
     6481 cttcatcaag gcgatgagcg gcgagatcac cagcgtcgtg ccgccaagca cccgcgccgg
     6541 aagctgaaac gtgagcgact tgccggcccc ggtcggcatg atgccgacgc agtctttgcc
     6601 cgaaagcacc gactcgatga tgggcagttg ccccggccgg aatgagtcgt agccgaaggt
     6661 gtccttcaaa acgcgctcca acgcggagcg gctgggtggt tctgcgagcg attgagcgac
     6721 cgacttgagc atgccgacga ggtcggtcga gaacccattc gggttgcgtc gatactcatc
     6781 acgcagcgac cgaagccccc gatccacctc ggcgcgccgc tggagatccc cgccacgaag
     6841 cgcttgggcg acgtcgttga gacgcgcgac gcggcgggcc aaggactcgg gttcggggac
     6901 ggacacgttg aagcggcgca atcctggcgc gagagccggg atcagtccaa ccggacccga
     6961 aaatgcgccg actatggcgc cctcacctcg agagcgcccc tcgcgcacgc acccggagag
     7021 aagcctcgac acggctgacg tttcgtgttg cgaacaccga cgcgctttga cgcccaccgt
     7081 ccaatgtcgt cgcacgcgcg ttgcgatccg tacgagctct cttcagtcga aacattccga
     7141 tgcgcctcga ggcgcgatga cgacgaggcg aggtcgagcg agcggcgcgc gcaattttgc
     7201 ggccgcgccg aagtcgccct tcggggttgc tgcgagtgag acgttggacc acagctccga
     7261 ccccggtggt tcgaaaaccg tcgggttgct cacgccgcgc atagattggt tggctcctcg
     7321 agccgtggtc gttagactgc ggttgcgtgg cggagacgtc gcgcgcaagc atgaaagccg
     7381 gttccgtcct ttcgggtaag tatcgcctcg tctccattct cggtcagggc ggcatgggat
     7441 cggtttggcg cgcggagcat ctcgcgctgc gcgctcccgt cgctgtgaag ttgctcgcgc
     7501 ctgctttcga agcgcgcccg gagacgtggg cgcgttttca ccgcgaagcg caatcggcgg
     7561 ccaacctgcg cagtccccac gtcgtccaga ttctggatca cggggtcgat caggaaacgc
     7621 aaacaccgtt catcgcgatg gagctgatgg agggcgagag cctcgccgag cggctgtccc
     7681 gtgtgggacg actctccccc gaagacacgc tcaaagtgat cacgcacacg gcgcgcgcgc
     7741 tgacccgcgc ccacgatgcc ggcattttgc accgcgacct gaagcccggg aacctcttca
     7801 tcgttcacaa cgacgacgac gagatcgtaa aggtgctcga ttttggtctc gccaagtggc
     7861 gcgtcgatcc cgagggacag cacgcggccg cgaccgtgac gggccagctg ctcggaacgc
     7921 cttactacat gagcccggag caggtcgaag catccaacca ggtcgattat cggagcgatc
     7981 tctggtctct gggagtcatc gcgtacgaat gtttggtggg ggcacggccg ttcgaaggcg
     8041 atagcctgat ggcgattgcg ttcaagatct gcgaacgaaa gctgtctccc ctacccgaga
     8101 cgctgccgct gccccgggct ctgaacgcat ggttcgagcg cgcgctcgct aaatcccccg
     8161 cagctcgctt tcaatccgcg cgcgaactcg cggaggactt gcgccgtgtt ttcggtgcac
     8221 ccacatcgca cgtgggcgcc gactccaccg ccgaaactgg cgccgctgcg gccgttcatc
     8281 gaccggcgca gagcccgccg ggcaacggag gagcagaagc cgacgctcga ccgagccagg
     8341 gcaccgtcga catgggacgg ccctcgttcg tccccccgca agctcccgag cgcttcgatg
     8401 caggtgagcc ggccgcgccc ccacccgctc ccgggttcga ggaaacagtc gaggccgtgg
     8461 tcgactacgc cgccgcgctc gggaagcgag ccgaggtcgc gccctccaat tcatcgtctc
     8521 cgaatgacgc gggcgaagcg gcctcggtcg agccaccgcc gatcagcgac cagagcgtgc
     8581 tgcccgtcgc gcggagcacc aatcgcgccg tcagagcgtc tgacaagcgc acgcctcggg
     8641 cgagcgtcgt gctcgtcgga gtcgccgtcg ccgcgctcgc cggagtcgcc gcgctcgcgc
     8701 tccgggggcc acgtggcacg aggtcgcccg atgctgcagc cgcgaacgtg gtcaacaatc
     8761 gagagcgcgt tgacgaacgc gcactcccat cgcgtctggt cgctccgact ccgggagtgc
     8821 gtgactctgc tgccacttcg acgaccgcgc ttccatcgca gccgtccgct ggtcccgtcg
     8881 ttgctccgga atccgctccg ccgctcgcag cgagcgctcc tcccctggtt gcagtggcat
     8941 ccgcttcccc ggccttgcct gccccattgg cgaagccctc gagcgcagcc ggaaccgctc
     9001 agcccaacgc acctgccggc gcactgaccc cgacgcgcga aaacacgccg aagacatcgg
     9061 cagcacccgc tccgaaagcc aagccgcgcg cgaacgtagg gcagagcaag gtttggctcg
     9121 aacgcgaata gcggcgcgga tggcctcctc gcgcgccaac gtcggagtct ccgcgaggag
     9181 cgtgccagc
//
LOCUS       DASTKS010000982         6983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_378246, whole genome shotgun sequence.
ACCESSION   DASTKS010000982 DASTKS010000000
VERSION     DASTKS010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378246"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFQ35_20750"
     CDS             complement(<1..962)
                     /locus_tag="VFQ35_20750"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HET9933151.1"
                     /translation="MKVPVSWLGQYFATPIDPDEIADRLTLAGIEVERIQGVGALDPL
                     VIVAEVSAIAPLPNGSFKLSLRADRARTVVSNAAGLAVGQRIALALPGATLFSESLDA
                     LVDVVESEVYSVVSQGMVAHRRALGIGDDTEQAVTFGSGAAPGSSVAAALSGAGLGSA
                     DRVLWLAILPNIARCQSVVGVAREIAALARVKLEPASEPPVLDAPNGLSPTIEARDAA
                     SVLRVTLLENVRVGESPRYVKERLLLGGMTPINNVVDASNYVMLEVGQPTHPYDADLL
                     ASLDLGVRRARPGDRLLTLQQAEGDEPMALPEGVPLIVSKDAPVA"
     gene            complement(1002..2039)
                     /gene="pheS"
                     /locus_tag="VFQ35_20755"
     CDS             complement(1002..2039)
                     /gene="pheS"
                     /locus_tag="VFQ35_20755"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9933152.1"
                     /translation="MQAEPENDLESYSKTALADLATAHDEKAIEAWYRKHLAPSGVMN
                     QWRKRIGSQPPEQRKAFGAEVNTRANALKEAFDARKREVAELALVESIAKQSVDVTLP
                     ARPQTSAGYHPVTQTIREVERLFHEMGFLTFESPHVELDEFNFGLLNMPEDHPARDMQ
                     DTFYLSDRTVLRTHTSAGQVRAMRIYAPHPVRVILPGLCYRNENITLRSEIQFHQIEG
                     LMVGKSVRLSDLKGVILSFARRMFGRDQRIRMRGSYFPFTEPSVEVDIRCTLCNGQGC
                     RVCKHSGWLELMGAGLVHPVVLSNGGYDPREVRGIAFGMGIERLVMLLHGIDDIRHFF
                     QNDLRFLRQFR"
     gene            2162..2950
                     /locus_tag="VFQ35_20760"
     CDS             2162..2950
                     /locus_tag="VFQ35_20760"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9933153.1"
                     /translation="MRLVSIIALDDRAKTESFGVDSALAQHVYGYWRLSVVDPPARLR
                     VVPDGHVDLVFDPELREAYVAGPRDTPLEVEHARATDLLGATLSPGSAMALLSIEGRA
                     LSPDWTPLDAVLGELGRALAERVADAPSTLLRVSVIETFLLARLAPKDLRLERALSVI
                     DSSEGRVAVAELGRESGASPRNLSRLFHAWVGLSPKRFARIARAQAALRRLSEIETGD
                     LGALAAELGFADQAHLTRELKAIAGAAPSHLAETFKRKSDSFKR"
     gene            3015..3371
                     /locus_tag="VFQ35_20765"
     CDS             3015..3371
                     /locus_tag="VFQ35_20765"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9933154.1"
                     /translation="MSNIVLSAITLHTEEPARLADFYRKVLELPLDLNQHGTLGSHFE
                     GTRGGVHFAIWKASTRVGGPVVPVFRVPNLERELERVRSLGVVELHRPLDLGEGKRVV
                     TLEDSDHNAFRLIEIS"
     gene            3686..4858
                     /locus_tag="VFQ35_20770"
     CDS             3686..4858
                     /locus_tag="VFQ35_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933155.1"
                     /translation="MTGPLTLLAATAFSAYHVVSTDVTHPTPALERVVHQVQVGQSPL
                     NRFAITEVHRHGRSKGTLILLSPFLLPGAFYEISEAGGYGKSAAGRLAESGIDVWLVD
                     QRRTGLAPGACESGAADCSVMLDWDFDTYSTDALFALSLVKTKGGCQKPVIGGFSAGA
                     NTALATLNRAPREFAGVFLYEGTFFTEDPTIRAHNDAACSTLDSLLASGSSFDASAAV
                     PGLALRLAAADPNGAFPVPAFPPGTTNEQAMFYVFGSPPPPGALSPTPNFVRCITDFP
                     NQRFVYTNEARLKLAGPLFDTYASLPAVRDLACGLAGRDDSHYDNVGAFRGKALIFVE
                     GTGFGPAMFDTAGLFDPRVDVTIESNPELGEADPYFHRRWSQVFLSPLSRWLQQVF"
     gene            complement(5114..6280)
                     /locus_tag="VFQ35_20775"
     CDS             complement(5114..6280)
                     /locus_tag="VFQ35_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9933156.1"
                     /translation="MLLRYCACVGFVVAGIAGVACGSGGESGDGSGTGGGISLGMGGG
                     VGISMGGAGPTAGNGSGASAGLDPNNACTGTSVEGERVPVDLYFMVDTTGSMNCPVPD
                     SVDNPCTVDPGPPYAATTRWVVESAALKSFMESKSNAGLGVGIGFFPASKDACNAATY
                     QQPAVEIALLPGAASQLTAAVTAKKPAGNTPTVASLTGAVQHATTYAKAHPGHRVAVV
                     YSTDGYPVSCGSDNTIANAANVAKGAFQGNPSIPVYVLALGNNLSSLNDIAVAGGTNQ
                     AFLIDTSRDAAAQLSAALDAIRGHAVVGCTYEIPPPPAGQQINYNLVNVRLTSGGKVT
                     QVYQDPSASCKDGWQYSADKTQINLCGDVCTQVKSDPDVKLEVLFGCQTQVVPR"
BASE COUNT         1183 a         2349 c         2225 g         1226 t
ORIGIN      
        1 gcgaccggcg cgtctttcga aacgatcagc gggacgccct ccggcagcgc catcggttcg
       61 tcgccctctg cttgttgcaa cgtcagcagg cgatcgccgg ggcgtgcgcg gcgaaccccg
      121 agatcgaggc tcgcgagcag atcggcgtca tacgggtgcg tgggctgccc gacctcgagc
      181 attacgtagt tcgaagcatc gaccacgttg ttgatgggcg tcatcccccc gagcaggagc
      241 cgctctttca cgtagcgggg cgactccccg acccgcacgt tctcgagcaa agtcacgcgc
      301 agcacgctcg cggcgtcacg cgcttcgatc gtgggagaga gcccgttcgg cgcgtcgagc
      361 acggggggtt cggacgcggg ctcgagcttc accctggcga gggctgcgat ctcacgggcg
      421 acaccgacca ccgactggca gcgcgctatg ttcggcaaga tcgcgagcca aagtacgcgg
      481 tcggcgcttc cgaggcccgc cccggagagc gcagccgcga ccgacgaccc tggcgcggcg
      541 ccactaccga aggtcacggc ctgctccgtg tcgtcgccga taccgagcgc acgacggtgc
      601 gcgaccattc cctgcgagac gacgctgtac acttcgctct cgaccacgtc cacgagcgcg
      661 tcgagcgact ccgagaacaa cgtcgccccg ggcagagcga gcgcgatgcg ctgtcccacc
      721 gcgagacccg ctgcgttcga aacgacggtg cgcgcgcggt cagcccgcaa gctcaacttg
      781 aagctcccat tcgggagcgg cgcgatcgcg gagacctccg ccacgatcac caacggatcg
      841 agcgcgccga cgccctgaat gcgttcgacc tcgatgcccg ccagcgtcaa gcgatcggcg
      901 atttcgtcgg ggtcgatcgg cgtagcgaaa tactgtccta gccaggaaac ggggaccttc
      961 atgatgctct tgtctcgccc tcttcgtgag tgtcgcgcgg ctcagcggaa ctgacgcaag
     1021 aaacgcaagt cgttctggaa gaagtgccgg atgtcgtcga tgccgtgcag cagcatcacc
     1081 aggcgctcga tccccatgcc gaacgcgatg ccgcgcacct cgcgtgggtc gtagccaccg
     1141 ttcgagagga ccacggggtg gacgaggcct gcgcccatca gctcgagcca accgctgtgc
     1201 ttgcagacgc ggcaaccctg cccgttacag agggtgcagc ggatgtcgac ctcgacgctc
     1261 ggctcggtga aggggaagta gctcccgcgc atgcggatgc gctgatcacg gccgaacatg
     1321 cgccgcgcaa agctcagaat cacgcccttc aggtcgctca ggcggacgct cttgccgacc
     1381 atcaggcctt cgatctggtg gaactgaatc tccgagcgga gggtgatgtt ttcgttgcga
     1441 taacacagtc ccggcaagat gacgcgcacg ggatgaggcg cgtaaatgcg catggcccgc
     1501 acttgcccgg ccgaagtgtg cgtacgcagc accgtgcgat ccgacaggta gaaggtgtct
     1561 tgcatgtccc gagcgggatg gtcctcgggc atgttcaaga ggccgaagtt gaactcgtcg
     1621 agctcgacgt gcggcgactc gaaagtgaga aagcccatct cgtggaagag ccgctcgacc
     1681 tcgcgaatcg tttgcgtgac cggatggtag ccggcgctcg tctgtggccg cgccggaagc
     1741 gtcacgtcca cgctctgctt cgcgatgctc tcgacgaggg cgagctcggc cacctcgcgc
     1801 ttgcgggcgt cgaacgcctc cttcaaagcg ttggcgcggg tgttcacttc ggccccgaac
     1861 gccttgcgct gctccggcgg ttgggaaccg atgcgcttgc gccactgatt catgacgccg
     1921 gagggcgcca gatgctttcg gtaccaggct tcgatcgcct tctcgtcgtg cgcggtcgcg
     1981 agatcggcga gcgccgtctt gctgtagctc tccaggtcgt tttcgggctc ggcttgcact
     2041 gacttctcgt ccttcgggcg ctgctatacc acggcgcgcc ggggcggccc gaatctcggt
     2101 tttttcgggg ttgcgagagc gccctttgcc gcgcgctcgg ggcgaattag cattcggcaa
     2161 gatgcgcctg gtttcgatca tcgcgctcga cgatcgggcc aaaaccgaga gtttcggggt
     2221 cgactccgcc ctcgctcagc acgtctatgg gtactggcgg ctctcggtcg tcgacccgcc
     2281 cgcacgcctt cgcgtcgtgc ccgacggcca cgtcgacttg gtgttcgacc ccgagcttcg
     2341 cgaagcgtac gtcgcggggc cgcgcgacac ccccttggaa gtcgagcacg cacgagccac
     2401 ggacctgctc ggcgcgacgc tgtcgccagg ttcggcgatg gcgctcctat cgatcgaggg
     2461 gcgcgctcta tcgcccgact ggaccccgct cgacgccgtg ctcggcgagt tgggtcgagc
     2521 gctcgccgaa cgcgtcgccg acgcgccgag cacgttgctc cgcgtgagcg tcatcgagac
     2581 cttcctgctc gcgcgcctcg cgccgaagga tcttcgactc gagcgagccc tctccgtcat
     2641 cgattcgagc gagggccggg tcgcggtcgc cgagctcggt cgcgaaagcg gggcgagccc
     2701 gcggaacctg agtcgcctct tccacgcttg ggtcggactc tcgccgaagc gcttcgcgcg
     2761 tatcgcgcgg gcccaagcag cgctgcggcg cctgtccgag atcgaaacgg gcgacctcgg
     2821 tgcgctcgcg gccgagctcg gcttcgccga tcaagctcac ctcacgcgag aactcaaggc
     2881 catcgccggc gccgcgccat cgcatttggc cgaaacgttc aaacggaagt ccgattcgtt
     2941 caagcgctga attgcgcacc gaagcaaggt ctcttcagaa cgacgcaccc cgcgtcgccg
     3001 aggagacatt cccaatgagc aatatcgttt tgtccgcgat cactctgcat acggaagagc
     3061 cggcgcggct cgctgacttc taccgcaaag tcctcgagct cccgctcgac ttgaaccaac
     3121 acggtacgct cggctcacat ttcgagggaa cgcgaggcgg cgttcacttc gccatatgga
     3181 aagcgtcgac gcgggtcgga ggtcccgtcg tgcccgtatt tcgggtaccg aacctcgagc
     3241 gcgagctcga gcgcgtgcgg agcttgggcg tcgtcgagct gcatcgcccg ctggacctgg
     3301 gcgaggggaa gcgcgtcgtg acgctcgagg actctgatca caatgcattc cgtctgatcg
     3361 aaatcagttg agactcgagt tctccgcacg aacgtacaac gcgcgcgaaa gcgccccagc
     3421 atctcgcagt gagaataccg ctggaatacc gcgcttccgc gcgcgtcgac gtcgccacga
     3481 acgcgccgag tcacccagct gggttatgcg tcgaaaaccg acgccgtcac tcgacgtgcg
     3541 cgaacgtagt caattattcg gaaaagctcg cgccctcccg ctgcgacgaa aaatgtctga
     3601 gcacctctga tacgatattt cgaagaaggg ggttttgcga tatgatgaag aatcgcgcac
     3661 ttcgcgaagt gagggagagc acaacatgac gggtccattg acgcttttgg ctgcaacggc
     3721 gttcagcgcc taccacgtcg tctcgacgga cgtaacgcac ccgacgccag cgctcgaacg
     3781 cgtcgttcac caggtgcagg tcggtcagag tccgctgaac cgctttgcga tcaccgaagt
     3841 gcaccgccac ggccgctcca aaggcaccct cattctcctg agtccgttcc ttctaccagg
     3901 cgcgttctac gagatctcgg aggcgggcgg ttacggcaag tccgcggcag ggcgcctcgc
     3961 cgagtccggc atcgacgtgt ggctcgtcga ccaacgacgt acgggcctcg ctccgggcgc
     4021 gtgcgagtcc ggcgccgcgg attgcagcgt catgctggat tgggatttcg acacctattc
     4081 gaccgacgcg ctgttcgctc tgtctctcgt gaagaccaag ggcgggtgtc aaaaacccgt
     4141 catcggcggc ttctcggcgg gagccaatac ggcgctcgcg acgttgaacc gcgcgccacg
     4201 tgagttcgcg ggcgtgttct tgtacgaggg aaccttcttt acggaagacc ccaccatacg
     4261 cgcgcacaac gacgcggcgt gcagcacgct cgacagtctg ctcgcgtcgg gatcgtcctt
     4321 cgacgcgagc gcagccgttc cggggctcgc gttgcgactc gcagccgcgg accccaacgg
     4381 cgcctttccg gtgcccgcct tccctcccgg cacgaccaac gaacaagcga tgttctatgt
     4441 gttcggctcg ccgcctccac ccggagcgct ctcgccaacg cccaacttcg tgcgctgcat
     4501 caccgatttc ccgaaccaga ggttcgtgta cacgaacgag gcccgcttga agctcgcggg
     4561 gccgctgttc gacacgtacg cttcactgcc cgccgtgcgt gacctcgctt gcgggctcgc
     4621 gggtcgcgac gactctcact acgacaacgt gggcgctttt cgtggcaagg cgctgatctt
     4681 cgtcgagggc accggcttcg gccctgccat gttcgacacg gctgggctct tcgatccgcg
     4741 cgtggacgtc accatcgaga gcaaccccga gctcggcgaa gcagacccct acttccaccg
     4801 gcgatggagc caggtcttcc tctcgccgct cagtcgctgg ctgcagcagg ttttctagca
     4861 gcgcgcgagg ggtcgttctc acgcaatacg aaaacgaaca ggccggcgct cgaaagcgga
     4921 tggcctgttc gcgtccgttc gctgagcacg ccggagctcg agagggggcg gcccactcac
     4981 gtcccttcgc tgagcggggc ggcgctcgca agcaaacgac gcgctcggtg cctacaccgt
     5041 gaccgttcga gaggaccgca agaggtcacc cgtcgggcga gaccgccaat ttcgcggtcc
     5101 tttggccagt ctctcagcgg gggacgacct gcgtctgaca gccgaacagc acttcgagct
     5161 tcacgtcggg gtcgctcttc acttgcgtgc agacgtctcc gcaaaggttg atttgggttt
     5221 tgtcagcgga gtactgccag ccgtctttgc aggacgcaga cggatcctgg tagacctgag
     5281 tcaccttgcc accgctcgtg agcctcacgt tgacgagatt gtagtttatc tgctgccccg
     5341 cgggtggcgg agggatttcg tacgtgcagc cgacgaccgc gtgaccgcgg atggcgtcta
     5401 gcgcggcgct caactgcgcc gccgcgtctc gcgacgtatc gatcaagaac gcttggttcg
     5461 taccgccggc aacggcgatg tcgttcagcg acgagaggtt gttgccgagg gcgagcacgt
     5521 aaactgggat actcgggttg ccttggaatg cgccttttgc gacgtttgct gcgttcgcga
     5581 tggtgttgtc gctaccgcag ctcaccgggt acccgtccgt cgagtagacc accgcgacac
     5641 ggtggccggg gtgagctttg gcgtaagtcg tggcgtgctg aacggcgccg gtcaacgaag
     5701 cgaccgtcgg cgtgttaccg gctggcttct tcgccgtcac ggccgcggtg agctgcgagg
     5761 cagcgccagg caacagcgca atctcgaccg cgggttgctg gtacgtagcg gcgttgcatg
     5821 catctttcga ggcagggaaa aagccgatgc cgacgccgag cccggcgttc gacttcgact
     5881 ccatgaatga cttcagcgcg gcgctctcca cgacccagcg cgtcgtcgcc gcgtaaggag
     5941 gaccagggtc caccgtgcag gggttgtcca ccgagtcggg aaccgggcag ttcatcgatc
     6001 cggtcgtatc gaccatgaag tagaggtcca ccggaacgcg ctctccttcc accgaggttc
     6061 ccgtgcaggc gttgttcggg tcgagccccg ccgacgcgcc gctgccgttg cctgccgtag
     6121 ggcccgctcc gcccatcgag atgccgacgc cgccgcccat tccgaggctg atgccgccgc
     6181 ctgtgccgga gccgtcacca gattcgcctc ccgacccgca tgcgacgcca gcgatacccg
     6241 caacgacaaa ccccacacac gcgcaatatc gcagcagcac ttgaaacctc cgatgcagcg
     6301 cgtggtggcg cctacaaggc gctcgcgctc gaggattgag gataacagcg tgccccatcc
     6361 acgcaacgcg ccaactccgc gagatcgaga gtctcatgct ccgaggtgcg cgcgcttcac
     6421 cacacacatc acccgcgcga gttgtcgtga cgtgtagccg cgaggcgtcc gaccgcgggt
     6481 gcgagcgcct ctcatcgcat gagcgggcaa gcgcctcggg tcacacgctg accacgcgag
     6541 tgaggtcgcg ggcgcatctt gtcagacccg cgccgacact cggttctcgc gctccgtgcg
     6601 ggtggcttgc ccccatatat cgttaatcga tacgttcgga gcgcgttcgc ccatgcctgt
     6661 catccggccg gttccggagc cctttgcagc gagaaccctc ctgctctcgg cccccgcgcg
     6721 gcacgacgca gccagggaag gcccgtcagg cccgggcggc gagatcgctc ggcggaaccc
     6781 ggccttcgtt ccctcgggcg acgacgacga ggacggccga accaagcgac ttgccggctc
     6841 ggcgaggcgt tgctcggaac gagtatgctc tcagtcccgt cccgaacggc tacgcgcgcg
     6901 gaggctcgta gccctgctta cagctcgccg acacaccgct gatgagcgcg cccacggtga
     6961 acgagaacgt atcccccagg ctg
//
LOCUS       DASTKS010000983         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1048573, whole genome shotgun sequence.
ACCESSION   DASTKS010000983 DASTKS010000000
VERSION     DASTKS010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048573"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1049
                     /locus_tag="VFQ35_20780"
     CDS             57..1049
                     /locus_tag="VFQ35_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933157.1"
                     /translation="MRPQVRCTRLIALLFVCAEAASCSSVNEHPGGEGGGGAASKGGS
                     SASGAGNHASGGAGAVGGSAGSGGKTEATGGIAEQGGGAGEPSGGVGAESGGPSTGGG
                     NIGGTGVGGFSTGGSDTGGTSAGGSGSGGGNTGGTASGGSGAGGTNGGSGSGGAASGG
                     SGSGGDNTGGTSGGSGGGGGNGGASDECARPFEIYSVTLTGSFYWESLNPTSCVASSG
                     SSPQASLLAQLMSGSDSMVATFSYGPVNFNGLIATRTATATGFTASFQGMCAPMSTAN
                     NSGTVTLTVDRTTGTVSVTGYCGRSGLSCAAPKTRTYGSFSGSGQWCTPEPNPD"
     gene            complement(1142..1660)
                     /locus_tag="VFQ35_20785"
     CDS             complement(1142..1660)
                     /locus_tag="VFQ35_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HET9933158.1"
                     /translation="MDLRFTQPSLRKLDLSGTEVLVAGLHSDERPPHGVAGLVDWRLG
                     GRISKLIKSGFATGTLGEVLLVPGKPKLPYDKVLLFGLGRSIEFSELTFRTVLERILK
                     TLEGLRARTAVVELPGRHRDALPAERAADILLEVAGARSEHDVWTLVESAEAQRIITQ
                     HMIQERRRVRSG"
     gene            complement(1670..2110)
                     /gene="nusB"
                     /locus_tag="VFQ35_20790"
     CDS             complement(1670..2110)
                     /gene="nusB"
                     /locus_tag="VFQ35_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9933159.1"
                     /translation="MGARSTAREAALQMLFALDASGAPADVVISDYWRETPGDAEGRA
                     YADELVRGIARDLESTDTRIRTASKNWRLERMTRVDRNVLRLGAWELEHRLDVPRAVI
                     LDEAVEIAKRYGTEESSSFVNGVLDRIADDCGRKDGDAPPETSA"
     gene            complement(2114..2611)
                     /gene="ribE"
                     /locus_tag="VFQ35_20795"
     CDS             complement(2114..2611)
                     /gene="ribE"
                     /locus_tag="VFQ35_20795"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9933160.1"
                     /translation="MADSSEVQVIEGSFSVPEGARFAIVASRFNAFIVERLVEGAVDG
                     LRRHGVVSSHITVVRVPGSWEAPLVCARLAKSKKFDAVIALGAVIRGATAHFDYVAGE
                     AAKGAAAASAASGVPVLFGVLTTDTIEQAVERAGTKAGNKGFDAALAALEMVSLGRAL
                     GDAGY"
     gene            complement(2712..>3904)
                     /gene="ribB"
                     /locus_tag="VFQ35_20800"
     CDS             complement(2712..>3904)
                     /gene="ribB"
                     /locus_tag="VFQ35_20800"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET9933161.1"
                     /translation="HDSVRPLDIGPEPLRRVQAAIEDIRAGKMIILVDDEDRENEGDL
                     CLAADAITPDAINFMCIHGRGLICLTLTEEQVERLDLPMMSAPGRNGPRLGTAFTVSV
                     EASEGVTTGISASDRAHTIRVASNPKAKPSDVVVPGHTFPLVARHGGVLVRAGQTEGS
                     VDLARLAGLNPAGVVCEIINDDGTMARMPDLERFAEKHDLKILTIADLIAYRLQTERQ
                     VERVSEGEIMLDRTSSPWHALVFEAVVDRRQILACIKGDLSGDEPVLCRMHSGSLIGD
                     VFSSAATEGGRNLGEAIDMIEKEGRGVIVYLPPRADLASELASVLALEAKSEAKSAPQ
                     NAPASARARPHGGTLREYGLGAQVLRALGVRRLRLLTNNPKKIAGIQGYGLDLVESLA
                     LDGH"
BASE COUNT          639 a         1295 c         1343 g          627 t
ORIGIN      
        1 ccctagtgag gacgcggcga gtggttactc gagaagagag ccacgggagg tcgaacatgc
       61 gaccgcaagt tcgttgcact cggttgatcg cgctcctgtt cgtctgtgcc gaagccgcct
      121 cgtgtagctc ggtcaacgaa catcccggcg gtgagggagg cggcggggcc gcgagcaaag
      181 gaggctcgtc agcttccggc gccggcaatc acgcaagcgg cggagctggc gcggtcggcg
      241 gctccgcggg cagtggcggc aagacagaag cgaccggtgg gatcgcggag caaggtggcg
      301 gcgcgggcga gccctcggga ggcgtcggag cggagtcggg cggaccgtcg acgggcgggg
      361 gcaacatcgg aggcaccggc gtgggcgggt tcagcaccgg tggcagcgac acgggaggca
      421 ccagcgcggg tggctctggc tcgggtggag gcaacaccgg tggcaccgcc tcgggcggct
      481 ccggcgccgg aggtaccaat ggtgggtcgg gctcgggcgg tgccgcgtca ggtggctcgg
      541 gctcgggcgg agacaacacc ggtggcacca gcggcggtag cggcggtggc ggcggcaacg
      601 gcggcgcgtc tgacgagtgt gcgcgcccgt tcgagatcta ctccgtaacg ctgacgggaa
      661 gcttctactg ggagtcgctg aacccaacct cctgcgtcgc ttcttccggc tccagcccac
      721 aagcgtcgct cctcgcgcaa ctcatgtccg gttccgactc gatggtggcc acgttctctt
      781 atggaccggt gaacttcaac gggctcatcg ccactcgaac cgcgaccgcg accgggttca
      841 cggcgtcatt tcaagggatg tgtgcgccga tgtcgactgc aaacaacagc ggcacggtga
      901 cgctaacggt ggacaggacg accggcactg tgtcggtgac ggggtattgc gggcggagtg
      961 gcctcagttg cgccgccccc aagactcgca cgtacgggtc cttcagcggc agcgggcaat
     1021 ggtgcacacc agaacccaat cccgactagc cgagcacgcg aaggcctctt ctgctccagc
     1081 gcactcgcgt gcgttcgacg aaactcgtcc atcgccgcgg tagagatcgt tcgctggaga
     1141 gctatcccga acgcacgcgg cgccgctctt ggatcatgtg ctgcgtgatg atgcgctgcg
     1201 cttcggctga ttcgaccagc gtccacacgt cgtgctcgct ccgcgcaccc gccacctcga
     1261 gcaagatgtc tgcggcgcgc tcggctggca gtgcgtcgcg atgacgcccc gggagctcga
     1321 cgacggccgt acgcgcgcgc agaccttcga gcgtcttcag aatgcgctcg agcacggtgc
     1381 gaaaggtgag ctcgctgaac tcgatgctgc gcccgagccc gaacaagagc accttgtcgt
     1441 aaggcagctt gggcttaccg gggacgagca gcacctcccc gagggtgccc gtcgcaaagc
     1501 ctgatttgat cagcttcgaa atgcgtccgc cgaggcgcca atcgacgaga cccgcgactc
     1561 cgtgcggcgg tcgctcgtcg gagtggagcc ccgcaaccag cacctcggtg ccggacaaat
     1621 cgagcttgcg cagggacggt tgcgtgaagc gaaggtccac gccgaaccct cacgccgacg
     1681 tttctggcgg cgcgtcgccg tctttgcgcc cgcagtcgtc ggcaatgcga tcgagcacgc
     1741 cattcacgaa actgctgctc tcttcggtgc cgtagcgctt cgcaatttcg acggcctcgt
     1801 cgagaatgac ggcgcgcggc acgtccagac gatgctcgag ctcccacgcc cccaggcgaa
     1861 ggacgttgcg atcgacacgg gtcatgcgct cgaggcgcca gttcttgctg gcggttcgga
     1921 tgcgcgtgtc ggtgctttcg agatcccgcg cgatgcctcg cacgagctcg tcagcgtacg
     1981 cgcggccctc tgcgtcgccc ggcgtttcgc gccaatagtc gctgatcacg acgtcggcgg
     2041 gagcgcccga ggcgtcgagc gcgaacagca tctgcaacgc tgcctcgcgg gctgtagagc
     2101 gcgcgcccat ggctcagtat cctgcgtcgc cgagcgcgcg accgagcgag accatctcga
     2161 gggcggcgag cgccgcgtcg aagcccttgt tgccagcctt ggtgccggcg cgctcgacgg
     2221 cttgctcgat ggtgtcggtc gtgagcacgc cgaacagcac cggcacgccg ctcgcggccg
     2281 acgcggcggc ggcgcccttc gcggcctcac cggcgacgta atcgaagtgc gccgtcgcgc
     2341 ctcgaatcac cgcgccgagg gcgatcacgg catcgaactt cttgctcttc gcgaggcgcg
     2401 cgcagacgag cggcgcctcc cacgagccag gtacgcgcac gaccgtgatg tgagaagaaa
     2461 ccacgccgtg gcgccgcaag ccatcgacgg cgccctcgac caaccgctcg acgatgaaag
     2521 cgttgaagcg cgaggccacg atggcgaagc gcgccccctc gggaacgctg aagcttcctt
     2581 cgatgacctg aacctctgag ctgtctgcca tgggatgcct tgccgcgcgc caaccgccgc
     2641 ggggagccgg ttctatcaca ttgttccgcg tcggcatgac cgggcggaac gaccctgaaa
     2701 tgccgcgagg ttcagtgccc gtcgagcgcg agcgactcga ccaggtcgag gccgtagccc
     2761 tgaatgccag cgatcttctt cgggttattg gtgaggaggc gaagcctccg aacacccagc
     2821 gcacgcagaa cctgcgcccc gaggccgtac tcgcgcagcg tgcccccgtg cggtcgcgct
     2881 cgtgcggacg ccggcgcgtt ctgcggcgcg ctcttcgctt cggacttggc ctcgagcgcc
     2941 agcaccgaag ctagctcgga cgcgagatcg gcccgcggcg gcaaatagac gatcacgccg
     3001 cggccctctt tctcgatcat gtcgatcgct tcgcccaggt tgcgaccacc ttcggttgcc
     3061 gccgagctga agacgtcgcc gatcagcgac ccggaatgca tgcggcacaa gaccggttcg
     3121 tcgccgctca ggtcgccctt gatgcaagcc aagatctgcc gacggtcgac caccgcctcg
     3181 aacaccaacg cgtgccaggg gctcgaggtg cgatcgagca tgatctcgcc ctcgctgacg
     3241 cgctcgacct gacgctccgt ctgcagccgg tacgcgatga gatccgcgat cgtgaggatc
     3301 ttgagatcgt gcttctcggc gaagcgctcg agatccggca tgcgggccat ggtgccgtcg
     3361 tcgttgatga tctcgcagac gacaccggcg gggttcagcc ccgcgagacg cgccagatcg
     3421 acggagcctt cggtttggcc ggcgcgcacg agcacgccac cgtggcgcgc gacgagcggg
     3481 aaggtgtggc ccggcacgac gacgtcgctc ggcttggcct tggggttcga agcgacgcga
     3541 atggtgtggg cgcgatccga ggcgctgata ccggtggtga cgccttcgct cgcctcgacg
     3601 ctcaccgtga acgcggtgcc gaggcgcgga ccgttgcgcc ccggcgcgct catcatcggc
     3661 aagtcgagcc gctctacctg ctcttcggtg agcgtcaggc aaatcagccc gcgcccgtga
     3721 atgcacatga agttgatcgc atcgggggtg atggcgtcgg cggcgaggca aaggtcgcct
     3781 tcgttctcgc gatcttcgtc gtcgaccaag atgatcatct tgccggcgcg gatgtcttcg
     3841 atcgcagcct gcacgcgacg caggggctct ggaccgatgt ctaacggacg cacggagtcg
     3901 tggg
//
LOCUS       DASTKS010000984         7105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_378332, whole genome shotgun sequence.
ACCESSION   DASTKS010000984 DASTKS010000000
VERSION     DASTKS010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378332"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1212)
                     /locus_tag="VFQ35_20805"
     CDS             complement(82..1212)
                     /locus_tag="VFQ35_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933162.1"
                     /translation="MNADASSVVGAGLEASEPSVASALIGRSLRLCDELLIPGLGFVL
                     SLALFGVFIAFTGNPPLEVLEQMYRGAFGTWFSFQNTLLRAAPLMLTGLCTALPARLG
                     LMVIGGEGALVVGGVAAAAVAQPLQGAAPLVVAVSMALAAMAMGALWIGAAGALRAFR
                     GVNETISSLLLAYIGIALLNHLVEGPLKDPQSLNKPSTRHIGEANMLPNLPNLDVHYG
                     LLFGVVACVACYVLMAHTTFGFAARVVGGNARAARLSGLSVEKLVVITCALAGAAAGL
                     AGMVEVAAVHGKANASLVAGYGYTGILVAFLARQNPLGVIPVALLLGGISASGGLLQR
                     TFQLPDATVNVLQGILFVVLLASETFQGRLRLFQRLEARFGS"
     gene            complement(1227..2414)
                     /locus_tag="VFQ35_20810"
     CDS             complement(1227..2414)
                     /locus_tag="VFQ35_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9933163.1"
                     /translation="MEDRRFSYVGVARRGFIVSASLALLSAWGCSKKSSDTPSAAASV
                     SSAVSAAQPAPSDALTVGFIYVGPKDDYGYNQAHAEGAGAVKKTPGVKVREEERVPET
                     VDVQKTMESMINLDGAKLLFPTSFGYFDPHILKLAEKYPAVTFFHCGGLYTEGKHPKN
                     VGSYFGYIDEAEYVAGITAGLTTKTNKIGFIAAKPIPQVLRNINAYTLGARSVNPKVT
                     TTVIFTGDWSLPVKEAEAANSLVDQGADVLTAHVDSPKVIIETAERRGVFSTGYHASQ
                     AKLAPKGYLTGAEWNWEVVYNDYISKVKKGEPWSHLVRGGFKEGFIKMSPYGAAVSDD
                     ARRKADEAKEKLTAGNFAIFKGPLKDNKGKELLAAGTEQGQTDINLEKMDYLVEGVKG
                     STN"
     gene            2832..4133
                     /locus_tag="VFQ35_20815"
     CDS             2832..4133
                     /locus_tag="VFQ35_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933164.1"
                     /translation="MNKVDSLVTRLVGALAAMVPATALAQAPAPAPAAPAPAPAAPPA
                     VAPAAPPPAAAAPAPAPAPVAPPPVAVAPPPAALPPPPPADAPPPPPPPPPAEEKMPS
                     IDVGAWLRAGIRFQGATDPKKINDQAMDAVYGELHLSGKITPKVSYTLNFNANGLAAK
                     AGIMDAIIGLDPIDEFHFWAGQLLVPVDRSNFSGPFFMSPWNYPGVFSVGGAGGFVGP
                     NEGPSGRNTGGVVWGDIGKGTFKYYAGMMNLSNRSERPLYSGRLAFAAIGKEPGYYGS
                     STYYGDQDILAFGVGAQYQKKGSVGATDPTTMVAPTDDFSDINADVLAEFKLGDSGVL
                     TGEAAYYHLTGDYNLADNNFFVLASYLSPDVGIGKIQPMVRYQWAKKKGADDPMSVID
                     AFVTLVIKGYTWRATAGFQHTDLGNDVVGNAIQIGLQTIQF"
     gene            complement(4228..4845)
                     /gene="ureG"
                     /locus_tag="VFQ35_20820"
     CDS             complement(4228..4845)
                     /gene="ureG"
                     /locus_tag="VFQ35_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET9933165.1"
                     /translation="MDKEPLRVGIAGPVGSGKTALVDALCKHLRDRYDMAVVTNDIYT
                     REDAEFLTRSGALPVDRIMGVETGGCPHTAIREDASINLDAIERMCKTHPKLDLLIVE
                     SGGDNLAATFSPELVDLTIYVIDVAGGDKIPRKGGPGITKSDLLVINKTDLAPMVGAS
                     LEVMDRDAKKMRGEKPFVFSCLKKGDGLENIANFVIREGMLPARN"
     gene            complement(4907..5593)
                     /locus_tag="VFQ35_20825"
     CDS             complement(4907..5593)
                     /locus_tag="VFQ35_20825"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET9933166.1"
                     /translation="MAEITLSASALLGALRLGSAALPIGAFAYSQGLERAVSLGHVRD
                     SETAERWIGGLLSHCVLTLDVPFLRALYEAWRAGDEAEVGRLSDLLFAMRGSLELRDE
                     ERQLGRALLRLLSRLGVERAARFGEREHPTLAAAFALSAVSWEIPSDAAALAYCFAWA
                     EAQVGAATRLVPLGQSAAQGVLSRVLKLAAEGVPPALTRTEATVSTTAPGQALFSAAH
                     ETEYSRLFRS"
     gene            complement(5586..6068)
                     /gene="ureE"
                     /locus_tag="VFQ35_20830"
     CDS             complement(5586..6068)
                     /gene="ureE"
                     /locus_tag="VFQ35_20830"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HET9933167.1"
                     /translation="MRTFDELLPRGDAREATLVLPLDHEQRRRARLRVMLPSGEAIGI
                     SLPRGGSLRDGDKLRASADGTVARVRAAPEHVSVAETSDAHLLTRGAYHLGNRHVALR
                     IERVRLIYPHDHVLDGLCRELGLSVSQQILPFEPEPGGYAGEHSHGGRLRPEPGAGRG
                     "
     gene            complement(6074..>7105)
                     /locus_tag="VFQ35_20835"
     CDS             complement(6074..>7105)
                     /locus_tag="VFQ35_20835"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET9933168.1"
                     /translation="TPAAIDNCLNVAEKYDVQVAIHTDTLNECGFVEDTLAAFKGRAI
                     HTFHTEGAGGGHAPDIIKACGAPNVPPSSTNPTRPYTVNTVDEHLDMLMVCHHLDPSI
                     AEDVAFAESRIRRETIAAEDILHDLGAFSMMSSDSQAMGRIGEVVTRTWQTAHKMKAQ
                     RGPLPEDSERNDNFRARRYIAKYTINPARTHGIAHEVGSLEVGKLADIVLYKPAFFGV
                     KPSLVIKGGFIAAAPMGDANASIPTPQPVHYRPMFGAFGKARDATSVLFVSEVSLERG
                     VADALRLARRLVAVRGCRNVSKADMKLNSYLPKMEVDPETYEVRADGQLLTCEPATVL
                     PLAQRYFLF"
BASE COUNT         1260 a         2343 c         2252 g         1250 t
ORIGIN      
        1 agaggaacgg cgcgctcacg cgcaacgccc cgccgaagac agcgagtggc actccccaaa
       61 cgccgatttc acccgccgcg ctcatgaccc aaaccgcgct tcgagcctct gaaacagccg
      121 caagcgcccc tgaaacgtct cgctcgcgag caagacgacg aacaggatgc cttgaagcac
      181 gttcacggtc gcgtccggca actggaacgt gcgctggagc agcccgccgc tcgcgctgat
      241 accgcccagc aggagggcaa cgggaatgac cccgagcgga ttttgacgcg ccaaaaacgc
      301 gaccaggatg cccgtgtaac cataaccagc cacgagggat gcgtttgctt tgccgtgcac
      361 cgccgcgact tcgaccatgc cggcgagccc cgcggccgcc ccggcgaggg cacacgtgat
      421 gacgacgagc ttttctaccg agagcccgct cagccgcgca gctcgggcgt tgccgccgac
      481 gacccgcgcc gcgaatccga aggtcgtgtg cgccatgagc acgtagcaag cgacgcacgc
      541 cacgactccg aacaaaagcc cgtagtggac gtccaagttc ggcaagttcg gcagcatgtt
      601 ggcctcgccg atgtgccgtg tggatggttt gttgaggctc tgtggatctt tcaagggccc
      661 ctcgaccaag tgattgagga gcgcgatccc gatgtacgcc aacagcaagc tgctaatggt
      721 ttcgttcacg ccgcggaagg cgcggagcgc gccggcggcg ccaatccaaa gcgcgcccat
      781 cgccatcgcc gcgagcgcca tgctcacggc gacgacgagc ggtgcggccc cttgcagcgg
      841 ctgcgcaacc gcggccgccg caacgccgcc caccacgagc gcgccctcgc caccgatcac
      901 catcagcccg agccgcgcgg gcaacgccgt acaaaggccg gtgagcatca ggggcgcggc
      961 gcgcaacaac gtattttgga aggaaaacca ggtcccgaag gcgccgcggt acatttgctc
     1021 gagcacttcg agcggtgggt tgcccgtgaa cgcgatgaat acgccgaaca acgccaacga
     1081 aaggacgaag ccgagccccg ggatcagaag ctcatcgcaa agccgcagag agcgcccgat
     1141 tagcgcggac gcgacgctcg gctcggacgc ttccaaaccg gcaccgacaa ccgacgaagc
     1201 gtccgcgttc attcgaagag cctcactcaa ttggtcgagc cttttacgcc ttcgaccagg
     1261 taatccatct tttcgagatt gatgtcggtc tgaccttgct cggtgccggc cgcgaggagc
     1321 tccttgccct tgttgtcctt cagcgggccc ttgaagatgg cgaagttacc agccgtgagt
     1381 ttctccttgg cttcgtccgc tttgcggcgc gcgtcgtccg acaccgcggc cccgtacggc
     1441 gacattttga tgaatccctc tttgaaccca ccgcgcacga ggtgagacca gggctcgccc
     1501 tttttgacct tgctgatgta gtcgttgtaa acgacttccc agttccactc ggccccggtg
     1561 aggtagccct tcggcgccaa cttggcctgg ctcgcatggt agccagtcga aaatacgcca
     1621 cggcgctcgg cggtctcgat gatgaccttc ggagagtcca cgtgtgcggt gagcacgtcc
     1681 gcgccctggt ccacgaggct gttcgccgcc tccgcttctt tcaccggaag cgaccagtcg
     1741 ccggtgaaaa tgacggtcgt cgtgaccttc ggattcaccg agcgcgcccc gagcgtgtaa
     1801 gcgttgatgt tgcggagcac ctggggaatc ggcttcgccg cgatgaagcc aatcttgttc
     1861 gtcttggtag tgagtcccgc cgtgatgccg gccacgtact cggcctcgtc gatgtatccg
     1921 aagtagctgc cgacgttctt ggggtgcttt ccctccgtgt agaggccgcc gcagtggaag
     1981 aacgtaaccg cggggtactt ctccgcgagc ttcaggatgt gcgggtcgaa gtagccgaac
     2041 gaggtgggga acaagagttt cgccccgtcc agattgatca tgctttccat ggtcttctgg
     2101 acgtcgacgg tctctggcac gcgctcttct tcgcgcacct tcactcccgg cgtcttcttc
     2161 accgcgccgg cgccctcggc gtgcgcttga ttgtacccgt agtcgtcttt cggaccgacg
     2221 tagatgaagc cgaccgtcag cgcgtcgctc ggcgccggct gcgccgcgga gacggcggac
     2281 gagaccgaag cggctgcgct cggcgtgtca ctcgacttct tgctgcaccc ccaagcgctc
     2341 agcagcgcga ggctcgcaga tacgataaac ccacgacgag caactccaac atacgagaaa
     2401 cgtcggtctt ccatgtgacc tgacgtatcg gaggagctcg gtttccgctg tgtgtcggac
     2461 cgaattccga acgcgcgggg ggcacttttg tcgacattgg cgcccgaacg ggcatcgctc
     2521 gagagggctt ttctgcggct gaatcgctcg atccgtgatc gccgagagtg gcgatttggc
     2581 ctcgtggcgg gactcgacac gtttgttacg cgcgaagctt tgccgacaca tggatttaac
     2641 acagcaaagg ggatgttccg cgcgacggtc ggtctctcgc gtgccgatcg atctgcacga
     2701 catgcactct cgtgaaactc ggtatttgag tcccttgcgg attctcaacc tgcgacagac
     2761 tcgattattc cgagcgtcgt gagagtcgcg ggcgaggtgc gcacaacgcg gaaccaagaa
     2821 gcggtgacag catgaacaaa gtcgattcct tagtgactcg tttggtaggc gcgctcgcag
     2881 ccatggtgcc agccactgcg ctcgctcaag ccccggcacc ggcaccagca gcaccagctc
     2941 cggcaccagc agcgccccct gccgttgccc ccgcagctcc tccgcccgca gcggctgcgc
     3001 cggcaccagc accggcgccc gtcgcgccgc cgccggtcgc ggttgcaccg ccgcctgcgg
     3061 ctctgcctcc tccacccccg gctgacgcgc cgccgccccc accgcccccg ccgccggccg
     3121 aagaaaagat gccgtcgatc gacgtcggtg cgtggctccg cgcggggatc aggttccagg
     3181 gcgcgaccga tcccaagaag atcaacgacc aggcaatgga cgccgtttac ggtgagctcc
     3241 acctgagcgg caagatcact ccgaaggtca gctacaccct gaatttcaac gcgaatgggc
     3301 tcgccgccaa ggccggcatc atggacgcga tcatcggcct cgacccgatc gacgagttcc
     3361 atttttgggc tggccaactg ctcgtcccgg tggatcgctc gaacttcagc ggtccgttct
     3421 tcatgagccc ctggaactat ccgggtgtgt tcagcgtcgg cggcgctggc ggcttcgtcg
     3481 gtcccaacga aggtccgagc ggccgcaaca ccggaggcgt cgtctggggc gacatcggca
     3541 agggcacgtt caagtactac gccggcatga tgaacctctc gaaccgcagc gagcgcccgc
     3601 tttacagcgg tcgactcgcc ttcgcggcga ttggtaaaga gcccggctac tacggcagca
     3661 gcacgtacta cggggaccaa gacattttgg ccttcggcgt cggcgcgcag taccagaaga
     3721 agggcagcgt tggagcgacc gatcccacga cgatggttgc gccgaccgat gacttctcgg
     3781 acatcaacgc cgacgtgctc gccgagttca agctgggcga cagcggagtc ctcaccggtg
     3841 aagctgcgta ctaccacctc accggcgact acaacctcgc tgacaacaac ttcttcgtgc
     3901 tcgccagcta cctttcaccg gacgtcggga tcggcaagat tcaaccgatg gtccgctacc
     3961 aatgggcgaa gaagaagggc gcggatgacc cgatgagcgt catcgacgct ttcgtgacct
     4021 tggtcatcaa gggctacacg tggcgcgcga cggccggctt ccagcatacg gatctgggca
     4081 acgacgtcgt cggcaacgcg atccagatcg gcttgcagac gatccagttc tgatgacggc
     4141 tcggtgagag ggcccaccgg tttgcacctc cccgaccggt gggctctctc gcacctcgcg
     4201 cgagcgtcgt tcaagcgcga gcgtcgttca attacgagcg ggaagcatgc cctcgcgaat
     4261 cacgaaattc gcgatgttct cgaggccgtc gcctttcttc aggcagctga agacgaacgg
     4321 cttctcaccg cgcatcttct tggcgtcgcg atccatgacc tcgagtgatg cgccgaccat
     4381 cggagccaga tccgtcttgt tgatcaccaa aaggtcgctc ttggtgattc ccggcccgcc
     4441 cttgcgcggg attttgtcgc cacccgcgac gtcgatcacg taaatcgtca agtcgacgag
     4501 ctcggggctg aacgtcgcag cgaggttgtc gccgccgctc tcgacgatga gcaagtcgag
     4561 cttcgggtgc gtcttgcaca tacgctcgat tgcgtcgagg ttgatggacg cgtcttcgcg
     4621 aatggccgtg tgcggacagc cgccggtctc gacgcccatg atacgatcga ccggcagcgc
     4681 gccactgcgc gtcaggaact cggcatcttc ccgggtgtaa atgtcgttcg tgacgacggc
     4741 catgtcgtag cggtcgcgca agtgcttgca cagcgcgtcg accagagcgg tctttccaga
     4801 ccctacgggc cctgcgattc caactcgaag cggctctttg tccatgttca gttcctctcg
     4861 catcgtgcgc tcgggtggtt tgggccatcc caggatggac tcgaggctat gagcgaaata
     4921 gcctcgagta ttcggtctcg tgcgcagccg agaacagcgc ttgccccggc gcagtggtgc
     4981 tcacggtcgc ttcggtgcgt gtcagcgcag ggggcacgcc ctctgccgcg agcttcaata
     5041 cgcgggagag cacgccctgg gccgcgcttt gaccgagcgg cacgagtcga gtcgcggcgc
     5101 cgacctgcgc ctctgcccac gcgaagcagt aggcgagcgc ggctgcatcg ctcggaattt
     5161 cccagctcac cgccgacagc gcgaaggccg ccgcgagggt cgggtgctcg cgctcgccga
     5221 agcgtgccgc tcgctcgacg ccgagccgcg agagcagccg gagcagtgcg cggccgagtt
     5281 gccgctcctc gtcgcgaagc tcgaggctgc cacgcatcgc gaacagcaaa tccgaaagcc
     5341 tcccgacctc ggcctcgtcc ccggcgcgcc acgcctcgta aagagcgcgc aagaaaggga
     5401 cgtcgagcgt gagtacacag tgcgagagca gccccccgat ccagcgctcg gctgtttcgc
     5461 tatcgcgcac gtggcccagc gacaccgcgc gttcgaggcc ttgcgagtag gcgaacgcac
     5521 cgatggggag cgcggcgctg ccgaggcgca aagctccgag cagggcggag gccgagagcg
     5581 tgatctcagc cacgaccagc gcccggctcg ggtctcaagc gcccgccgtg cgagtgctca
     5641 cccgcgtagc cgcccggttc cggctcgaac ggcaagattt gctgcgagac gctcaggccg
     5701 agttcgcgac acaagccgtc gagcacgtga tcgtgcggat agatgaggcg cacccgctcg
     5761 atgcggagcg ccacgtgccg attgccgagg tggtaagcgc ctcgggtcaa gagatgcgcg
     5821 tccgaagtct cggcgaccga gacgtgctcc ggtgccgctc gcactcgcgc gaccgtgccg
     5881 tcggcgctcg cgcgcagctt gtcgccgtct cgcagcgaac cgccgcgcgg caaagaaatg
     5941 ccgatcgctt ctccgctcgg tagcatcacg cgcaggcggg cgcggcgacg ttgctcgtgg
     6001 tcgagcggta gaaccagcgt cgcctcgcgg gcgtctcccc ggggcaacaa ctcgtcgaac
     6061 gtgcgcatgc tggtcaaaac agaaagtagc gttgcgcgag cggcaatacc gtcgctggct
     6121 cgcacgtgag gagctggccg tcggcgcgca cctcgtacgt ttctggatct acttccatct
     6181 tcggcaggta gctgttgagc ttcatgtcgg ctttgctcac gttccggcag ccgcgcaccg
     6241 cgacgaggcg ccgcgcgagg cggagagcgt cagccactcc gcgctcaagc gatacctcgg
     6301 agacgaacag cacgctcgtc gcgtcgcgcg ccttgccgaa cgcgccgaac atgggccggt
     6361 agtgcaccgg ctgcggcgta ggaatcgaag cgttggcgtc gcccatcggc gcggccgcga
     6421 tgaagccgcc cttgatgacg agcgaaggct tcaccccgaa gaaagccggc ttgtaaagga
     6481 cgatgtcggc gagcttgcct acctcgagcg agcccacctc gtgcgcgatg ccgtgggtgc
     6541 gcgcggggtt gatggtgtac ttggcgatgt agcggcgagc gcggaagttg tcgttgcgct
     6601 cggagtcctc ggggagcggg ccgcgctgcg ccttcatctt gtgcgcggtt tgccaggtac
     6661 gggtcaccac ctcgccgatg cgacccatcg cctgcgagtc gctcgacatc atgctgaagg
     6721 cgccgagatc gtgcaaaatg tcttccgccg cgatggtctc acggcgaatg cggctctccg
     6781 cgaacgcaac gtcttcggcg atcgacggat ccaagtggtg gcacaccatc aacatgtcga
     6841 ggtgctcgtc gacggtgttc acggtgtagg gccgcgtcgg gttcgttgac gacgggggca
     6901 cgttcggcgc gccgcacgcc ttgatgatgt ccggagcgtg gcctccaccg gcgccttcgg
     6961 tgtggaaagt gtgaatggct cgccccttga aagccgcgag cgtgtcttcg acgaagccgc
     7021 actcgttcaa ggtgtcggtg tgaatcgcga cctgaacgtc gtacttttcg gcgacgttca
     7081 ggcagttgtc gatggcggcc ggcgt
//
LOCUS       DASTKS010000985         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_156030, whole genome shotgun sequence.
ACCESSION   DASTKS010000985 DASTKS010000000
VERSION     DASTKS010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156030"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="manA"
                     /locus_tag="VFQ35_20840"
     CDS             complement(<1..842)
                     /gene="manA"
                     /locus_tag="VFQ35_20840"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET9933169.1"
                     /translation="MNFSPLLCKIQRYAWGSRSFIASLQGRPTPSDEPEAELWVGAHP
                     SAPAEVVEGETQSRLDVLIARAPERALGTDVARRFHDELPFLMKVLAAAEPLSLQAHP
                     SRAQAEQGFAQEQAAGIRLDAPTRSYKDARPKPELIVALTPFVALSGFRPLERTLRLI
                     AELSIPELTGLFGPLHAGTGLAADGAEARLARVFDRLINSEAAERDSLVRSVRVRAAE
                     LAPQSKEFARELRWVERLYTLYPGDIGVVISLLLNHVELAPFQGLYLPPGNLHAYLDG
                     AGVE"
     gene            complement(842..1789)
                     /locus_tag="VFQ35_20845"
     CDS             complement(842..1789)
                     /locus_tag="VFQ35_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933170.1"
                     /translation="MRTLGLSVLLVACAPAAPAPPSAPVPRSSAAPVATTPGAAATPT
                     PAAPVTSAPSSGAATPSAKPCGELACLEFPSAEGAFERVLSEEPRVLAVGEAHAQKGS
                     ETVASTAKRFGEQLLPLVRGRASDIVIELLLANGSCGKKTEERVAEKQKPVTENQAAT
                     NQNEFVELGTIAKKQGTMPHALTPTCDEFAAISSAGDDVVDRMLTTVAAASTRNLEAL
                     LKQRPPSALLLAYGGALHNDLAPRPGRERWSFGPHFSEVTGGKYVELDLIVPEYVKDT
                     ETWRAFPWYSAYAHAPASHGALLYTVAPHSFVLIFPRST"
     gene            complement(1894..>2669)
                     /locus_tag="VFQ35_20850"
     CDS             complement(1894..>2669)
                     /locus_tag="VFQ35_20850"
                     /inference="COORDINATES: protein motif:HMM:NF025428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HET9933171.1"
                     /translation="RSSRERVCRKVGSSRSRASRFSDGFYAPAAPRAWISEDRAPDAA
                     ALVARLANAFSTTGLWPLVLESLNDDADERPWLAAELSPAPYRVSSTRDAQETLANWW
                     SDVFPTEDEVFDDFKPFSREFPGLAAAPPAGRDSVPIHEVAAVLSGRLALVPLTRPAD
                     APAALGWTGPINHYGDMGLLSCVLRSWEERFEAFVVGIGFDTLTLAVGRPPSTLDAAR
                     SVAAEHFATCPDTVYQGCGSIEALAQDLLDSETWTFWWD"
BASE COUNT          463 a          830 c          937 g          439 t
ORIGIN      
        1 atttcgacac cggctccgtc gaggtaagcg tggagattcc ccgggggaag atacaggcct
       61 tggaaagggg cgagctcgac gtggttcagc aggagcgaga tgacgacgcc gatatcacct
      121 ggatagagcg tgtagagtcg ctcgacccaa cgaagctcgc gcgcgaactc cttggattgc
      181 ggagcaagct cggcggcgcg aacacgcacg ctacgaacga gcgaatcgcg ctcagcggcc
      241 tcgctgttga tcagtcgatc gaaaacacga gcgagccggg cttcggcccc gtctgccgcg
      301 agccctgtgc ctgcgtgcaa cggaccgaag agcccggtga gctcggggat cgagagctca
      361 gcgatgagac gcagcgtacg ttcgaggggg cgaaagccgg agagcgcgac gaagggcgtg
      421 agggcgacga tgagctcggg cttggggcgt gcatctttgt agcttcgcgt gggcgcatcg
      481 aggcggatgc cggcggcttg ctcctgggca aagccttgtt ccgcttgcgc gcggctcgga
      541 tgcgcttgga gcgagagcgg ttccgcagcg gcgaggacct tcatcaagaa cgggagctca
      601 tcgtgaaagc gtcgcgcgac atccgtcccg agcgcgcgct cgggagctcg ggcgatcagg
      661 acgtcgaggc ggctttgggt ttcgccttcg acgacctcgg cgggggcgct cggatgcgcg
      721 ccgacccaga gctcggcctc gggttcgtca ctcggagtcg gtcggccctg caaggacgca
      781 atgaacgatc gggagcccca ggcgtacctt tgaatcttgc agagcagggg actgaagttc
      841 actacgtgga cctcggaaag atcagaacga aggaatgagg agcgacggtg tagagcagcg
      901 ctccatgact cgcgggcgcg tgagcgtacg ctgagtacca aggaaaggcg cgccacgtct
      961 ccgtgtcctt tacgtactcg ggcacgatga ggtccaactc gacgtacttg cctcccgtga
     1021 cctccgaaaa gtgggggccg aaggaccatc gctcccgacc cgggcgcggc gcgagatcgt
     1081 tatggagcgc gccgccgtag gccaaaagga gcgcggacgg cgggcgctgc ttgaggagcg
     1141 cctccaagtt gcgcgtgctt gccgccgcga ccgtcgtcaa catgcgatcg acgacgtcgt
     1201 ctccggcgct cgagatggcg gcgaattcgt cgcacgtggg ggtgagcgcg tgcggcatgg
     1261 tgccctgctt tttcgcgatc gtcccgagct ctacgaattc gttctgattg gtggccgctt
     1321 ggttttcggt gacgggcttt tgcttttcgg caacgcgctc ttcggtcttc tttccgcagc
     1381 tcccgttcgc cagcaaaagc tcgatgacga tgtcactggc gcgcccccgc acgaggggca
     1441 agagctgctc gccgaagcgt ttcgcggtcg acgccacggt ctcgctgccc ttttgagcgt
     1501 gcgcttcgcc gaccgcgagg acgcgtggct cttcgctcag cactcgctcg aaggctccct
     1561 cggccgaagg aaactcgagg caagcgagct cgccgcaggg ctttgcgctc ggcgtggcag
     1621 cgccgctcga gggcgcgctc gtcactggcg cggccggcgt cggtgtcgct gctgctcccg
     1681 gcgtcgtcgc gactggagcc gcggaggatc gggggacggg ggcactcggt ggtgccggcg
     1741 ccgccggcgc gcaggcgacg agcaagaccg aaagtccgag ggtacgcatg gtgttcgaca
     1801 ctcaatctag acccgctacg gtcggtttgc cttggccttc tcgggcttcg cgccgcagtg
     1861 cgggaaggcg ggaactatcg cgcgtcggcg ctgtcagtcc caccaaaagg tccaagtctc
     1921 gctgtcgagc aggtcctgcg cgagggcctc gatcgagcca cagccctggt aaacggtgtc
     1981 gggacaagtt gcaaaatgct ccgctgccac gctacgcgcc gcgtccagcg tcgatggtgg
     2041 gcgacccacc gcgagcgtca gcgtatcgaa ccctatcccc acgacgaacg cttcgaagcg
     2101 ttcctcccag gaacgcagca cgcacgagag gaggcccatg tcaccgtaat ggttgatggg
     2161 cccggtccag ccaagcgcgg cgggcgcatc cgcgggtcga gtcaacggaa ccagcgctag
     2221 ccttcccgag agcaccgccg cgacttcgtg aatcggcacc gagtcgcgcc ccgcgggagg
     2281 agcggcggcg agcccgggga actcgcggct gaacggtttg aaatcgtcga agacttcgtc
     2341 ttccgtcggg aagacatcgc tccaccaatt cgccagcgtc tcctgcgcgt cgcgcgtcga
     2401 agaaacgcgg tagggagccg ggctgagttc agccgcgagc catgggcgtt cgtcggcgtc
     2461 gtcgttcaac gactcgagga ccagcggcca caggcccgtc gtggaaaagg cgttcgcgag
     2521 ccgagcgacg agcgctgccg cgtcgggtgc acggtcttcc gaaatccatg cgcgtggggc
     2581 agcgggcgcg taaaaaccgt cggagaagcg actggctcgg gaccgtgatg agccgacctt
     2641 tcggcaaacg cgctcccgcg acgatcgcg
//
LOCUS       DASTKS010000986         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1272878, whole genome shotgun sequence.
ACCESSION   DASTKS010000986 DASTKS010000000
VERSION     DASTKS010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272878"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFQ35_20855"
     CDS             <1..561
                     /locus_tag="VFQ35_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933172.1"
                     /translation="TDGSPTLSLGCMNAGGQGMDVDPAPILSEVQRAADAEVQSFFIG
                     SPGSESNRAWLSQAAQIGGTPQPGCSPTGPTYCHLDMTTAADFSAALKSALNAILGVI
                     TPCTYTIPKPASGTIDPTKINVILTSNNQSTLVVRDDVSDCTQGWQLTSNNEILLCKD
                     TCDQARTAGMRVDVLFGCESLLRPPK"
     gene            complement(600..2753)
                     /gene="ppk1"
                     /locus_tag="VFQ35_20860"
     CDS             complement(600..2753)
                     /gene="ppk1"
                     /locus_tag="VFQ35_20860"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET9933173.1"
                     /translation="MSLSIDAIHSPLLATELYLNRELSWIAFNARVLDQAFDERWPLL
                     ERLKFLAIFGSNLDEFFMIRVSGLHDQLASNVESTGSDGLSVREQLSRIRRVVFGLSE
                     SAATLWSSVLLPALESVGICVRRHVDLSERAARWAREYFRREVFPVLTPLAVDPLHPF
                     PFLSNLSLSLAVEAEDPLSRDRRFARVKVPESLGRFVVIDADPTQAAGESPNVEILLL
                     EDLISANLEALFPGMRIVGCWPFRVTRDMDLEILEEEAADLLALVDREVRRRRFGAAV
                     RLEVAPGVPERIRALLLEKLELEADDLYEAEGPLGLAGLFAIANLARKDLHDAPLRQR
                     LPRGWSERNDPFACIAERDILLHHPYDSFTPVLDLLRAAADDPNVLAIKMTLYRAGMN
                     SEAVRALVRAAENGKQVAVAVELKARFDEEANILWARALERAGAHVFYGAPGLKTHAK
                     ALLVVRRESKGLCRYVHLSTGNYNASTARLYTDLALLTTNAEIGEDVAELFNALSGFA
                     KGASYRKLSVAPLALRPALLAKIEEQRARAARGEPAHIFAKLNSLVDSEIIRALYEAS
                     RAGVTIELMVRGVCCLRPRLAGVSESISVYSLIGRFLEHERVYAFGAPGAEQIFLSSA
                     DWMPRNLDRRVEALFPVEDARLIARIRDEVIAPLSRDNSRVYEMDSDGTYRRRRPERG
                     EELIDGQLYASERVHDLDAVRSNPRTVRESISASA"
     gene            2958..>3531
                     /locus_tag="VFQ35_20865"
     CDS             2958..>3531
                     /locus_tag="VFQ35_20865"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET9933174.1"
                     /translation="MMHRLDRRSFSLSIASAALALTGISCSKKEAPAADTQPAPVAST
                     VQAAAPEAITLQGSGASFPAPVYSRWFREFSAKNSGIRVNYQATGSGAGIKAFIAGQT
                     DFGASDAAMTDDEIKQAQGNVVLLPVTAGHVVLAYNLEGVKGLKLSREAYAGIFLGDI
                     KKWNDPKIAKANPGVKLPAQDITPVNRSDGS"
BASE COUNT          683 a         1116 c         1155 g          577 t
ORIGIN      
        1 acggacggga gtccgacgct gtcgttgggc tgcatgaacg ccggaggtca gggcatggac
       61 gtcgatccgg caccgatctt gagcgaggtg cagcgcgcgg ccgacgcgga ggtacaaagc
      121 ttcttcattg gctcgcccgg aagcgagagc aaccgcgcct ggctctctca agccgcgcag
      181 attggcggga ctccgcagcc gggctgcagc ccgacgggac ccacgtactg tcacctcgac
      241 atgacgaccg cggcggattt cagcgccgct ctcaaatcgg cgctcaacgc gattctgggt
      301 gtcattacgc cctgtacgta cacgatcccg aagccggcga gcggaacgat cgatcccacc
      361 aaaatcaacg tcattctcac gtcgaacaat cagagcactc tcgtcgtacg cgatgacgtg
      421 agcgattgca ctcaaggttg gcagctgacg tcgaacaacg agatcctgct gtgcaaggac
      481 acgtgcgacc aagctcgaac cgcggggatg agggtcgacg tgctctttgg gtgcgagagc
      541 ctgctgcggc cgcccaagta gctcggcgcg caggtcggcc ccgaaccaac ctcagtgcgc
      601 taggcgctcg cgctgattga ctcgcgcacg gttcgcgggt tcgagcggac ggcgtcgagg
      661 tcgtggactc gctcggaggc gtagagctgc ccgtcgatga gttcctcgcc ccgttcgggc
      721 cttcgccggc ggtaggttcc gtccgaatcc atttcgtaca cgcgtgagtt gtcgcgcgag
      781 aggggcgcaa tcacctcgtc gcggatgcgc gcgatgaggc gcgcgtcctc gaccgggaac
      841 agcgcctcga ctcggcggtc gaggttgcga ggcatccagt ccgcgctcga gagaaagatt
      901 tgctcggcgc cgggcgcacc gaaggcgtac acacgctcgt gctcgaggaa gcgcccgatc
      961 aacgaataca ccgaaatgct ctcggaaacg ccggcgagtc ggggccgcag acagcacacc
     1021 cctcgaacca tcagctcgat ggtcacgccg gcgcgagacg cttcgtagag cgctcggatg
     1081 atctcgctat cgacgaggga gttgagtttg gcaaagatat gagccggctc gccgcgcgcc
     1141 gcacgcgcgc gctgttcttc gattttcgcg agcagcgccg ggcgcagcgc gagcggggcg
     1201 accgatagct tgcgatagct tgcccccttc gcgaaaccgg agagggcgtt gaagagttcg
     1261 gcgacgtctt caccgatttc ggcgttcgtc gtgaggagcg cgagatccgt gtaaagacga
     1321 gcggtgctcg cgttgtagtt gccagtcgag aggtgcacgt accggcagag tcctttgctt
     1381 tcgcgccgca ccacgagcag cgccttggcg tgcgtcttca gcccgggagc gccgtagaag
     1441 acgtgcgcac cagcgcgctc gagcgcgcgc gcccagagga tgttcgcctc ttcgtcgaag
     1501 cgcgccttga gctctaccgc gaccgcaact tgtttgccat tctcggcggc gcgcacgagc
     1561 gctcgcaccg cctcggagtt catgccggcg cggtacaggg tcatcttgat cgcgaggacg
     1621 ttcgggtcgt cggcagccgc gcgcagcaag tcgaggacgg gcgtgaacga atcgtaaggg
     1681 tgatgcagca agatgtctcg ctcggcgatg cacgcgaacg gatcattacg ctcgctccag
     1741 cctcgcggaa gcctttggcg gagcggcgca tcgtgcaagt ccttgcgtgc gaggttcgcg
     1801 atggcgaaaa gtcccgcgag cccgaggggc ccttccgctt cgtacaagtc gtccgcctcg
     1861 agctcgagct tctcgagcag cagggcccga atccgctccg gcacccccgg cgcgacttcg
     1921 agacgcaccg ccgcaccgaa gcgtcgtcgc ctcacctcgc gatccacgag cgcgagcaga
     1981 tccgcggctt cctcctccaa gatctcgagg tccatgtcgc gcgtaacccg gaagggccag
     2041 caccccacga tgcgcattcc ggggaacagc gcctccaggt tcgccgagat cagatcctcg
     2101 agaagcagga tctctacgtt cggcgactcg cctgccgctt gcgtggggtc cgcgtcgatg
     2161 accacgaaac ggcccaagct ctccggcacc ttgacgcgcg caaaacggcg gtcgcgcgag
     2221 agcggatcct ccgcttcgac ggcgagcgac agcgacaggt tcgacaagaa cgggaacgga
     2281 tgcagtggat ccaccgcgag cggcgtcaaa accgggaaca cctctcgacg gaaatactcg
     2341 cgagcccaac gcgcggcgcg ctcggagaga tctacgtggc gtcgcacgca gattccaacg
     2401 ctttcgagcg cgggcaaaag cacgctcgac cacagcgtcg ccgcgctctc ggagagaccg
     2461 aaaacgacgc ggcggatgcg gctcagctgc tcgcgcacgg agagaccatc gctccccgtc
     2521 gactcgacgt tggacgcgag ctgatcgtga aggccggaga cgcggatcat gaagaactcg
     2581 tccaaattcg atccgaagat ggccaggaac ttgagccgct cgagcagcgg ccaacgttcg
     2641 tcgaaggctt gatcgaggac tcgcgcgttg aacgcgatcc aagagagttc ccgattcaga
     2701 tagagttcgg tggcgaggag cggcgaatga atcgcatcga tcgagagaga catggggctc
     2761 gtcaattggt aagtgtcggc cgcacgagtc gagttcaacc gtgtgagtga agtgaagcct
     2821 ttccacgcgg gagcttgtgt gaagggcacg tgacgatttc gtgtctcgtg ccctggcggg
     2881 atgacggggc actcgcgatc gcgtagtcca tgggcgacac cccggcagca ccgcgcgtcg
     2941 ggaaccaaag agcaaccatg atgcatcgtc tcgatcgacg ttctttcagc ttgtccatcg
     3001 ccagcgcagc gctcgccctg acgggcattt cttgttcgaa gaaggaggcg cccgccgccg
     3061 acactcagcc ggcaccggta gcatccacgg tacaggccgc cgctcccgag gcgatcacgt
     3121 tgcagggatc gggcgcgagc tttcccgcgc cagtctattc acgctggttt cgcgagttca
     3181 gcgcgaaaaa ctccggcatc cgcgtgaatt atcaggcgac ggggagcggg gccggcatca
     3241 aggcgttcat tgctggccaa accgacttcg gcgcgagcga cgcggcgatg acggacgacg
     3301 agatcaagca agcacaaggc aacgtggtgc tgctgccggt caccgcgggg cacgtcgtgc
     3361 tcgcgtacaa cctcgaaggc gtcaaggggc tcaagctctc gcgcgaggcg tacgccggca
     3421 tctttctcgg cgacatcaag aagtggaacg atccgaagat cgcgaaggcc aaccccggcg
     3481 tcaaattgcc agcgcaagac atcactccgg taaatcgctc ggatggaagc g
//
LOCUS       DASTKS010000987         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1719334, whole genome shotgun sequence.
ACCESSION   DASTKS010000987 DASTKS010000000
VERSION     DASTKS010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719334"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..544)
                     /locus_tag="VFQ35_20870"
     CDS             complement(317..544)
                     /locus_tag="VFQ35_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933175.1"
                     /translation="MLLREACCLAAEDPVLWTLFGVQCWRVNRRDEARQAIRQALWLR
                     ERMGDERRAHILRALLLAVESACAQSAVRAA"
     gene            complement(966..2456)
                     /locus_tag="VFQ35_20875"
     CDS             complement(966..2456)
                     /locus_tag="VFQ35_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933176.1"
                     /translation="MSEELTRDAGFTHGQDFVIGEISGALGLGPLEMQYEGLFAEALE
                     DGIITPEERARLERAADNLGLDRARLMRLEQAMLHAWQARHKVEIVEHYEDVPRSLSP
                     LRVEAEGDAGRALLLKRIEQLETRVRELEAELRRAQAAVNVEVDLSAVESAAETASQD
                     PEAVWRRLRRDPTNHDALHQLFDIYWARSDTDRAFCVAQALVLLEAASPDERAVFEQH
                     RMQTLIAPRNSISQAAWHDLLFHPEEEVLTGQIFATIAPAVLVGRVTALRRDRKLHQP
                     PAEARQDVQKSTLTAVRALPWAAAILGVQAPPIFVEREREVGYEHIPGVPPVTIVGKA
                     VLSGRSQLEHAFLAGRHLAMYRAEHYIKTLFHAVQDLEDLFLAALTIGSPGLPIADDM
                     KRRVQPIADAIAPMLESAQVDTLRGCFLRFVEEGGRTNLLRWATAVDKTACRAGLVLA
                     NDLNTACRVLHTEEGALGELGKDLICFVTSERYFRLRRQLGITVQP"
     gene            2586..3764
                     /locus_tag="VFQ35_20880"
     CDS             2586..3764
                     /locus_tag="VFQ35_20880"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9933177.1"
                     /translation="MRASFVALFIFPLACSPYAAPPTLPPPPPPPPTTSAAGPTSNRP
                     VATAAETKEAAATEKPASTPASGESVAASETPVAFPAFEIEPPYLPGKYPRPVGRMAA
                     SLADEELARWNVGGSSQSAHPSNRPNYHPAPRVVVDLRPLGRALPERATKSRFASAAS
                     LLAEARNQGYWPFRTCYEQGLRESAELSGQVQVRVRVAPSGRVASARLRGSPLKSHHT
                     VECVLRAASALTFKKPPARRVDADLSVKFWPGDAPLPPRDERVGVAWDPTAIAAAVSA
                     ESSDLARCCVDALARDPQLWGRLAFTVHTDSTGRVRESHETESRFPDSRASDCVREAL
                     ARLSQLPATPAQFVVALRCGQPAAPRPAVPAEAIPSENSDAAAPEPGETGSLPARSPD
                     "
     gene            complement(3774..5159)
                     /locus_tag="VFQ35_20885"
     CDS             complement(3774..5159)
                     /locus_tag="VFQ35_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933178.1"
                     /translation="MRRISKIRELGPSFLLALGALHAACSGKVTSGGTAGASNDASGG
                     APALRGGAANQSGGVRSAQGGSRNSESGGASTTASEGGRADGSRGGRSSVGRGGVGGA
                     NNDSTRGGRSASGGSSSSGGAGGTIRNANLGTACVNDSACGANLWCYDQPDVPQGFCT
                     ADCTRNSDCERLSPGSVCALDQMEGFCLQGCSVGSGSVTKCQQRDDFTCRLFAVDPGT
                     VCRGDADCAAIGPAYFCSSNTCIATACLPTCANDAECGTGYCDLGSGYCADTKPVGLP
                     LGSACDVSAAPDPCAGTCIGDTAGTFSLCSGYCNLGLATSCGWDGTGKADAACLFAPA
                     FNDSPGAGDAGFCAQLCDCNSDCQNPTFTCVALDTPEARVLKRAGYCTLAASTDTVIS
                     KCPGAGAGGAGGSGGAGSGSGGSAGASSASGGVGATSGLGGGGGVGGSSGSSGSGGTS
                     GVGGGSSGSSG"
     gene            complement(5354..>5729)
                     /locus_tag="VFQ35_20890"
     CDS             complement(5354..>5729)
                     /locus_tag="VFQ35_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933179.1"
                     /translation="GTFSLCSGFCNLGLATACGWDGTGKADAACLFAQGFNESPAAGD
                     SGFCGQLCDCNSDCRNPKFSCVALVDAEAKIVKRAGYCTAADPTDKVISQCPAGGGTG
                     GTGGASGGGTSAGGSGGAAGAG"
BASE COUNT          968 a         2002 c         1814 g          945 t
ORIGIN      
        1 agatcgggcg gcggggcgcg accttctact tttggtgcgc cgcagcccaa ggatgcgcag
       61 atcagaagca actcgatcgg attgattttg caatccgttc ggcgattgag cgccgtttga
      121 ggccaatcgt agcctccgag cgccaccccg tcgccgtgtc acccgcacgg tgggtcagct
      181 cgctgtccta tcccaaccca aaccccgcca gcttggcccc ttgggcgggg tggcaattga
      241 cgggatgagc ggcggccctc ttcactccaa ggagtgacac gagccgacgc cgatcaagct
      301 tcgagacgtc gacggctcac gcggcgcgaa cggcactttg cgcgcaagca gactcgaccg
      361 cgagcagtaa agcgcgcagg atgtgcgcgc gacgctcgtc ccccatgcgt tcacgcaacc
      421 agagtgcctg acgaatcgcc tggcgtgcct cgtcgcggcg gttcacgcgc cagcactgca
      481 ccccaaacaa cgtccaaagc accgggtcct cggcggcgag acaacatgct tcgcgcagga
      541 gcaccagagc tcggcggtgg tcgcctctcg cacgtcggcg gcgcgcctgt tccagacgct
      601 cgctagcgat tggcggccga ttcgctgcga tcccacgtgt ttccagctga ctcatgccgg
      661 acgagaatgc gaactccgtg ccgagctcga ctcgtacgca actccccgta ctcacgtact
      721 aagcggacag accgacgtgg cggaactcga acaaaaatcg ctaactcgcc tcagttgtgc
      781 tgtcttggcg ggctgggcgc cggtgtcaac aatcgaacgc cctgctcgag tcagaggcgt
      841 gacataagga aaggaccgag agatcagggc gtgcggctcc cgacccgacg gtagccgcca
      901 cggagctctc gtccgcctgg aagccaatcc gagcccctag acggtttagg attaccgatt
      961 ggggttcacg gctgaaccgt gatgccgagc tgccgtcgca gccggaagta gcgctcactc
     1021 gtcacgaagc aaatcaaatc cttgccgagc tccccgagcg cgccctcttc cgtgtgaagc
     1081 acgcggcagg cggtgttcag gtcattcgcg agcaccagcc ccgcccggca cgccgtcttg
     1141 tcgacggccg tggcccagcg gagcaagttg gtgcgtccgc cttcctcgac gaaccgcaga
     1201 aagcatcccc gcagcgtgtc gacctgagcc gactcgagca tgggcgcgat ggcatccgcg
     1261 atcggctgga cgcgccgctt catgtcgtcg gcaatcggta ggccggggct gccaatggtg
     1321 agggcagcga gaaagagatc ttctagatct tgaaccgcgt ggaagagcgt tttgatgtag
     1381 tgctcggcgc gatacatcgc aaggtgacgc ccagccaaga atgcgtgttc gagctgcgag
     1441 cgcccgctca gcacggcctt gcccacgatg gtcacgggag gaactccggg gatgtgctcg
     1501 tagccgacct cgcgttccct ttcgacgaag atgggcggag cttgtacccc gaggatggcc
     1561 gcggcccagg gcagcgcgcg aacagccgtg agcgtcgact tttggacgtc ctggcgagcc
     1621 tcggccggcg gctgatgaag ctttcgatct ctgcgcagcg cagtcaccct tccaacgagc
     1681 acggcggggg cgatcgtcgc gaagatctgg ccggtcagga cctcttcttc cggatggaac
     1741 aggaggtcgt gccacgcggc ttgagagatg ctgttgcgcg gcgcgatcag cgtctgcatc
     1801 cggtgctgct cgaagaccgc gcgctcgtcg ggagaggcgg cttcgagcaa cacgagggcc
     1861 tgagcgacgc aaaacgctcg atccgtatcg gagcgagccc agtagatgtc gaaaagctga
     1921 tgcaaagcgt cgtggttggt aggatcgcga cgcaaccgcc gccaaacggc ttcgggatct
     1981 tgtgacgccg tttcggcagc ggactcgacg gcgctcagat cgacttcgac gttgacggcc
     2041 gcctgcgcgc gccggagctc cgcctcgagc tcgcgcactc gagtctcgag ttgctcgatg
     2101 cgcttgagca gcagagcgcg tcccgcgtcc ccttccgcct cgacgcgcag tggggagagc
     2161 gaacgcggca cgtcctcgta gtgctcgacg atctcgacct tgtggcgggc ctgccaggcg
     2221 tgcagcatcg cctgctcgag gcgcatcaag cgcgctctat cgaggcccag gttgtcggct
     2281 gcgcgctcca gccgggctcg ctcctctggc gtgatgatgc cgtcttccaa ggcctccgcg
     2341 aacagaccct cgtactgcat ctcgaggggc ccgagcccga gcgcccccga aatctcgccg
     2401 atgacgaaat cctgtccgtg cgtaaagccg gcgtcccgcg tcagttcctc gctcatgtgg
     2461 ctccctcgtc ggtacccatc ccgacagaat tcgcgcaaag tgtctcagag ctagcgcggc
     2521 ccttcaaccc gaaagcgtgc aaaggcgggc ggcttcgcgc actcggtttc tctataatcc
     2581 gccgcgtgcg cgcatcgttt gtcgctctgt tcatttttcc gttggcgtgt agcccttacg
     2641 cagcgccccc cacactgccg ccgccaccac cacccccacc aaccaccagt gccgcgggcc
     2701 cgacgtcgaa tcggcccgtc gcgaccgcgg ccgaaacgaa ggaagcggca gcaaccgaaa
     2761 aacctgcgtc cacacctgcg agcggcgaaa gcgttgcggc gagcgagacg ccggtcgctt
     2821 ttcccgcgtt cgaaatcgag cctccgtatc tccctggtaa gtatccgcgc ccggttggtc
     2881 gaatggctgc gtcgctcgcc gacgaggagc tcgcgcgctg gaatgtcgga gggtcgagtc
     2941 agagcgcgca cccgagtaat cggccgaact atcacccggc accccgcgtc gtcgtcgatt
     3001 tgagaccgct cggccgcgcg ttaccggagc gcgccaccaa gtctcgcttc gcgagcgctg
     3061 cctctttgct cgcggaggct cgaaatcagg ggtactggcc gttccgcact tgctacgagc
     3121 agggcttacg agagagcgcc gagctctccg gacaagttca agtgcgcgtg cgagtcgctc
     3181 cgagcgggcg cgtcgcgtcg gcgcgtctgc gcggttcgcc gctcaagagt caccatacgg
     3241 tcgagtgcgt gttgcgtgcg gcctccgcgc taactttcaa gaagccgcct gcgcggcggg
     3301 tcgatgccga tctctccgtg aagttctggc caggggatgc tccgttgcca ccgcgcgacg
     3361 aacgcgtcgg ggtcgcgtgg gatcccacag caatcgccgc cgcagtcagc gccgagagca
     3421 gcgaccttgc tcgctgttgc gtcgatgcgc tggcccgtga cccacagctc tggggccgcc
     3481 tcgcgttcac ggtgcacacc gactcgaccg gacgcgtgcg agaaagtcac gagaccgaga
     3541 gtcgcttccc cgattcgcga gcgagcgatt gcgttcgaga ggcgctcgct cgcctttcgc
     3601 agctgcccgc aacccccgcg caattcgtgg ttgcgctgcg atgcggtcag cccgcggcgc
     3661 cgcgaccagc cgtacctgct gaagccattc ccagtgagaa cagcgacgct gctgcgccgg
     3721 agcccggaga aacaggctcg ctaccggctc gctcccccga ctagccgaag agctcagccg
     3781 gagctgccgg agctgccgcc gcccacgccg ctagtgccgc cgcttccgct gcttccgcta
     3841 gagccgccca caccgccgcc gccgcccagg ccgctcgtgg cgcccacgcc gccgctcgcg
     3901 gagctagcac cggccgaacc accactgcca gaaccagccc cacccgagcc accggcaccg
     3961 cccgcgccag cgccagggca cttcgagatc acggtgtccg tcgacgccgc gagcgtgcag
     4021 tacccagctc gcttcagcac tctggcttcc ggagtgtcga gcgcgacgca ggtgaacgtc
     4081 gggttctggc agtccgagtt gcagtcgcat agctgagcgc agaagcccgc gtcgccagcg
     4141 ccgggcgagt cgttgaaggc gggggcgaag agacacgccg cgtccgcctt cccggtgccg
     4201 tcccagccgc acgaggtcgc gaggccgagg ttgcagtagc cgctgcacaa agagaaggtg
     4261 cccgccgtgt caccgatgca ggtacctgca caaggatcag gcgctgcgct gacgtcgcag
     4321 gccgagccga gcggtaaccc cacgggctta gtatctgcac agtagccgct gccgagatcg
     4381 cagtagccgg tcccgcactc cgcatcgttc gcgcacgtcg gtaagcacgc ggtcgcgatg
     4441 caggtgttgg agctgcagaa gtatgccggc ccaattgccg cgcaatctgc gtcgccgcgg
     4501 cagacggtgc ctgggtctac tgcgaacagt cgacaagtga agtcgtcgcg ctgctggcat
     4561 ttcgtgaccg agccgctgcc gaccgagcac ccttggaggc agaagccctc catctgatcg
     4621 agcgcgcaca cgcttcccgg agacagccgc tcgcagtcgc tgtttcgagt gcagtcggcc
     4681 gtgcagaagc cttggggaac atcgggctgg tcgtaacacc aaaggtttgc gccacaggcc
     4741 gagtcgttga cgcacgccgt tccgaggttt gcgttccgaa tcgtgccgcc cgccccgccc
     4801 gagctgctcg agccaccgct cgcgcttctg ccgcctctcg tcgagtcgtt gttggcgccc
     4861 ccgacgccac cacgcccgac actgctcctg ccgcctcggc tcccgtccgc cctgccaccc
     4921 tcggacgcgg tggtcgaagc gccgccgctc tcgctattgc ggctgccgcc ctgagcactg
     4981 cggacgccgc cagattgatt ggcagctccg cccctgagcg cgggtgcgcc gccactcgcg
     5041 tcgttcgaag ctcccgcggt gccgcctgag gtcacctttc cagaacaggc cgcgtggagg
     5101 gcgcccagtg ctagcaaaaa gctcggaccg agctcgcgga tcttactgat tcgacgcatg
     5161 actcaccccg cttcacgcgg ccagaattga cgattcaaac cggcgcagac ctttgcgacg
     5221 aaagagcctg ctcagcacct ttggagcgcg ccgtgcgcga gcgctgcgaa cctcgatctt
     5281 ccgtcgagca cgactgcccc ccgcgtgtcg atgacggggg acagtctcca cggctcgctc
     5341 ttcgcgctat ggctcagccc gcgccagccg cgccgccgct gccgccagcg gaagtgcctc
     5401 cgccgctagc gccgccggtc ccgcccgtac cgccaccggc aggacactgc gagatcacct
     5461 tgtcggtcgg gtcggcggcc gtgcagtagc ccgctcgctt gactatcttg gcttctgcat
     5521 cgaccagcgc aacgcacgag aacttcgggt tcctgcaatc gctgttgcag tcgcagagct
     5581 gaccgcagaa cccggagtcg cccgccgcgg gcgattcatt gaacccctgc gcgaagaggc
     5641 aggccgcatc ggcctttccg gtgccatccc agccgcacgc cgtcgcgaga ccgagattgc
     5701 agaacccgct gcacagcgag aacgtgccg
//
LOCUS       DASTKS010000988         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1944253, whole genome shotgun sequence.
ACCESSION   DASTKS010000988 DASTKS010000000
VERSION     DASTKS010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944253"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="VFQ35_20895"
     CDS             <1..2113
                     /locus_tag="VFQ35_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933180.1"
                     /translation="LEQNRSEAPTAFTGAPIAMTGARFVAADSTGNVVNGPYGPVTLL
                     DEDKHALALARVASVDFEINAGAFPDIEVRAAVLDANGAPINGLSAPDFALSDQGKPQ
                     GATLLSNTAVTDVRVLLAYDGSSSVTWPTPAAREAFDTSISQAFVDATTQAPFSLCVA
                     GLGSASGPWSPPDATAIRNALSTLISSSPVWGTLGSAALAAGVSAVVVVSDFDADDDA
                     STVSALRQRLGASGLAVALVPVGNANQATIAAVVEACGALVLDPNANDFPAQLAAFVS
                     RRTLEIRSAAYRLRYRLPDDAASAAAARTVTLTCGAQPLTQSHDYVAPATGKAGLPPG
                     IGGLYLEVTANGATSTRRLSGPRLTRFGVPERPFSAEDIAETKHLLSGLVAIAFEPGS
                     PTTAANLEDVLSGVLSTEPASKAVGKDASAVAEAGAHFQFASGSLGMLAERAPSNARA
                     AVPSGVRVVIQTMFVDEGGLARRFDIVPAANRVLGVGTDEAAAFRAAVVASVVPSQRE
                     ASVSPGSAYARLATAELRFVAAFENPQALEGFTPADLAAWRSVLDQYGDWHRLVPTSP
                     SVGALWVVDPDTGTSVAVLLDGSGGASHSCTDAVNNAVVQAALQVVSLYATYKSFVCE
                     LAQTGGTLPCVGATVATVYAAGAALFTAAVLDSVSYADVVLLLIGMVTTQFPAAYGGL
                     LSLASLYVAKQEVADSLYEHC"
     gene            2177..2893
                     /locus_tag="VFQ35_20900"
     CDS             2177..2893
                     /locus_tag="VFQ35_20900"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9933181.1"
                     /translation="MPGDTPSAPATESAFAASSRSTLTRRDLARFEGDGLFRRVARTL
                     CQAECLPRKELFESWEIARRARRRFRGGRVLDLACGHALVAHLMLLLDDSSPKALAVD
                     ARLPSSAAKVADCFVREWPRLAHRVELVEASIASVAAEPGDVLVSCHACGTLTDDVLR
                     MALDARARVVVLPCCHDAARSDQGSLGGWLDSPLAIDVTRVARLRAHGYAVHTQLIPA
                     TITPKNRLILATPSERSAPL"
     gene            complement(2935..4275)
                     /locus_tag="VFQ35_20905"
     CDS             complement(2935..4275)
                     /locus_tag="VFQ35_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933182.1"
                     /translation="MGVALLACSFGCGQRASGGDGPPEGGSAAQGATAGRSSSSGSGG
                     VAGAAAVAGAGGSATAAQGGALAAGGGSNSGGASSASRGGTAGQSSGGAGKGSSSGGA
                     GPSGNGGTGGRAGSGSGAAAGRDGGAAGASNDFTFFAFADAHAGQNQKADSTLQTAMA
                     QMRRIDPNAAFGLSGGDLVEDSTTQAWSSHDAAVKASGLHPEATSFNGMARYFAAVDN
                     HDQGFPVMRNNWHTLWDQHLSGQASMGHDGTDGVYYTFSYQNALFIALDSIHPSAAQD
                     TWLAGVLSSTEAQNASVKIAFFHEPVYICTMDHPPFAAGLPWVDSFEKNGVKLAFTAH
                     SHIYERSCPMLHGRCSSERNAVVYQGVGPLAANDFRVVDRTTVKVSGKDAAGNARSDD
                     YACTGTDSIWRVNKTNTNTFCHVSVSGCRIAGECYVVSSDTSQPFDSWEVDGCR"
     gene            complement(4495..5046)
                     /locus_tag="VFQ35_20910"
     CDS             complement(4495..5046)
                     /locus_tag="VFQ35_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933183.1"
                     /translation="MQPCGGDVVGTWAIKDACLDTSAASSQIASTLMSGLGCTTATVQ
                     SADAKVSGSLTFNSDMSYQANVSQSTTIKLMLPSDCLKFQGITVTCDQLNQAVSSGLL
                     TGFDSFKCGSVSGGCTCTIGFSENDTSSGTYTASGNDLTFSNSQSLGGAYCVQSNQLH
                     MISVVMPMGSGETKILGDVVAAK"
     gene            5152..>5355
                     /locus_tag="VFQ35_20915"
     CDS             5152..>5355
                     /locus_tag="VFQ35_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933184.1"
                     /translation="MTILPAPPRKASVFRVAVEPSSTALARAEGALELLEPGTFAAMS
                     LVDKFTFEKRGARGVSDSHYAFAL"
BASE COUNT          899 a         1821 c         1677 g          958 t
ORIGIN      
        1 gctcgagcag aaccgctcgg aggcgcccac cgcgttcacg ggcgcaccca ttgcgatgac
       61 cggagcgcgc ttcgtcgcgg cggactcgac gggcaacgtc gtgaacggcc cttacggccc
      121 ggtcacgctg ctcgacgaag acaaacacgc gctcgcgctg gcgagggttg catccgtcga
      181 cttcgagatc aatgcgggcg cattccccga catcgaagtg cgcgcggcag tgctcgacgc
      241 gaacggagcg cccatcaacg ggttgagcgc gcccgacttc gcgctgagcg accaggggaa
      301 accccagggt gcaacgctac tctccaacac cgccgtcacg gatgtgcgcg tgctactcgc
      361 gtacgatggc agctcgagcg tcacttggcc tacgcccgcg gcgcgcgaag cattcgacac
      421 ctccatttca caggcgttcg tcgatgcgac cacgcaggcc ccgttttcgt tgtgcgtcgc
      481 cgggctcggg agcgcgagcg ggccttggtc gccacccgac gcgactgcaa ttcgaaacgc
      541 cctgagcacg ttgatttcgt cctccccggt ctggggtacc ctcggttcgg cagcgctcgc
      601 ggcgggcgtc tcggcggtcg tcgtcgtgag cgatttcgat gccgacgacg atgcaagcac
      661 cgtgtccgca ttgcgacagc gcctcggcgc gagcggcctc gccgtggccc tggtcccggt
      721 cgggaacgcg aatcaagcga cgatcgcggc cgtggtcgaa gcttgtggcg cgctcgtgct
      781 cgacccaaac gcgaacgact ttccggctca gctcgccgcc ttcgtgtcgc ggcgcacgct
      841 cgagattcgt agcgcggcct accggcttcg ttaccgcttg cccgacgacg cggcgagcgc
      901 ggccgcagcg agaacagtaa cgctcacttg cggagcgcag cctctcaccc aaagccacga
      961 ctacgtcgcg cccgccaccg gcaaggcggg gctgccaccc ggcatcggcg gcttgtacct
     1021 cgaggtgact gcgaacgggg ctacgtcgac tcgacgacta tcgggtccgc gcctcactcg
     1081 cttcggcgtg cccgagcgcc cgttcagcgc ggaagacatt gccgagacca agcacttgct
     1141 gagcggcttg gtggcgatcg ctttcgagcc cggaagcccg accacggcgg ccaatctgga
     1201 agatgtcctc tccggagtgc tgtcgaccga gccagcatcc aaagcggtgg gcaaagacgc
     1261 gagcgcggtc gccgaagcag gcgcgcactt tcaatttgct tcggggtcgc tcggcatgct
     1321 cgcggagcgc gcaccctcga atgcgcgcgc tgcggttccg agcggcgttc gcgtggtgat
     1381 tcaaacgatg ttcgtggacg agggcggctt ggcacggcgc ttcgacatcg ttcctgccgc
     1441 aaaccgcgtg ctcggcgtcg gaaccgacga ggccgccgcg ttccgagccg ccgtggtcgc
     1501 cagcgtcgtt ccgagccagc gcgaagcctc ggtttcgccc ggctcggcgt acgcgcgact
     1561 cgcgacggca gagcttcgct tcgtcgccgc tttcgaaaac ccacaggccc tcgagggctt
     1621 cacgcccgca gacctcgccg cctggcgctc cgtgctcgac caatacggtg attggcatcg
     1681 gctcgtgccg acgtcgccga gcgtcggggc gctttgggta gtcgatccag acacggggac
     1741 cagcgtagcg gtcctgctcg acggctccgg cggcgcctct cacagctgca cggacgccgt
     1801 gaacaacgcc gtggtgcaag cagctctgca agtggtgagc ctctacgcga catacaaatc
     1861 gttcgtgtgc gagctcgccc aaacgggggg aaccttgccg tgcgtgggtg cgacggtggc
     1921 gaccgtctac gccgctggag cagcgttgtt caccgcagcc gtgctcgact cggtgagcta
     1981 cgccgatgtc gtgttgctgc tgatcggtat ggtaacgacg cagtttccag ccgcgtacgg
     2041 cggcctcttg tctctcgcga gcctctacgt cgcaaagcaa gaggtcgcag attcgcttta
     2101 cgagcattgc tgagtcgagc tcggcaacac ctcgactttg ttgcgacagg ggcgcgaagc
     2161 cctgctactg agccgtatgc ctggcgatac cccctctgcg cctgcgaccg agagcgcgtt
     2221 cgcggcttcg agccgctcaa cgctgacgcg acgcgatctg gcccgcttcg agggcgatgg
     2281 cttgtttcgg cgagtggcgc gaacgttgtg ccaagccgag tgtttgccgc ggaaggagct
     2341 cttcgaatct tgggagatag cgcggcgcgc gcggcgcagg tttcgaggtg ggcgagtcct
     2401 ggatctcgcg tgtggacacg cgctggttgc ccatttgatg ctgcttctgg atgactcttc
     2461 gcccaaggcg ctggccgtgg acgcgcggct tccgagcagc gccgccaagg tggccgactg
     2521 cttcgtccgt gagtggccgc gcctcgcgca tcgcgtcgaa ctcgtcgaag cgagcatcgc
     2581 gagcgttgcg gctgaacccg gagacgtttt ggtgtcgtgt cacgcctgcg gcactctgac
     2641 cgacgacgtt ctgcgaatgg cgctcgacgc gcgggctcgc gtggtcgttt tgccgtgctg
     2701 ccacgacgcg gcgcgctcgg accaaggcag cctcggtgga tggctagact ctccgctcgc
     2761 gatcgacgta acgcgagtag ctaggctgcg tgcccacgga tacgccgtgc acacccaact
     2821 cattccggcc acgatcacgc ccaagaatcg gctcattttg gccacgccga gcgagcggag
     2881 cgccccgctc tgactcgacg agagccgcgc cgacgcccgc cagggctcga cgaactaccg
     2941 gcagccgtcc acttcccaac tgtcgaacgg ctgggacgta tcgctgctga cgacgtagca
     3001 ctcacccgcg atgcgacagc cgctgacgct tacgtgacag aacgtgttgg tgttcgtctt
     3061 gttcacgcgc caaatgctgt ccgtgcccgt gcaagcgtag tcgtcgctgc gcgcgttgcc
     3121 ggcggcgtcc ttgccactga ccttgaccgt ggtccgatcc acgacgcgaa aatcgttcgc
     3181 ggcgagaggc cccacgcctt ggtacacgac cgcgtttcgc tcgctgctac agcggccgtg
     3241 cagcattgga cagctccgct cgtaaatgtg cgagtgcgca gtgaacgcca gtttgacgcc
     3301 gttcttttcg aacgagtcga cccacggtaa ccccgccgcg aacgggggat ggtccatcgt
     3361 gcagatgtag acgggctcgt ggaagaacgc gattttgacc gacgcgttct gcgcttctgt
     3421 gctcgaaagc acgcccgcga gccacgtgtc ttgcgcagcc gaggggtgaa tgctgtcgag
     3481 agcgatgaac agcgcgttct gatagctgaa cgtgtagtag acaccgtccg tcccgtcgtg
     3541 gcccatcgac gcctgcccgg acaggtgctg gtcccacagc gtgtgccagt tgttgcgcat
     3601 caccgggaag ccctgatcgt gattgtctac ggcagcgaag taccgcgcca tcccattgaa
     3661 gctcgtcgct tctgggtgca gccccgaagc cttcacggcg gcgtcgtgac tgctccatgc
     3721 ttgagtcgta ctgtcctcca ccaggtcacc gccgctcaac ccgaacgcgg cgttggggtc
     3781 gatgcggcgc atctgcgcca ttgcggtctg aagagtcgag tcagcttttt ggttttgccc
     3841 tgcgtgagca tcggcaaatg cgaaaaaggt gaagtcgttc gatgcgccgg cagcgccacc
     3901 gtcgcgccct gcagctgcgc cgctgcctga gccagcacgg ccgcccgtgc cgccgttacc
     3961 gctcgggccg gcgccaccgc tgctcgagcc cttacccgct ccgccactcg actgccccgc
     4021 agtgccgccg cgcgaagcgc tcgacgcgcc gccagagttc gacccgccgc ctgcagccag
     4081 cgcgccgcct tgggcagccg tcgcgctacc gccagcgccc gctacggccg cagcccccgc
     4141 aacgccgccg cttccagaac tcgaggaacg ccccgcggtt gcaccttgcg ccgccgaacc
     4201 gccctcgggc ggcccgtctc caccacttgc gcgctgtcca cagccgaacg aacacgcgag
     4261 cagcgcgact cccacgcgac caacatagga gtgcagcctt ccacgcgttg acgcgcggag
     4321 ccatgaagcg cgaggttccc tcatcgagac cacgtcttta tgcagagcga cgtggcggca
     4381 cgagggtgtc acattcgtta ctgcgtcgtc accatccgcg taatgctttc cgatgaggca
     4441 agcagattgc ggcctcgctg cgcggcagaa atgcaggctg caacgcctgg gccgctattt
     4501 ggctgccacc acgtctccca aaatcttggt ttcacccgag cccatcggca tgactaccga
     4561 gatcatgtgc agctgattcg actgcacgca gtacgcaccg ccgagactct ggctgttgct
     4621 gaacgtcaag tcgttaccgg acgccgtgta cgtaccactg ctggtgtcgt tttcgctgaa
     4681 gccgatcgta caggtgcagc cgccgctcac cgaaccgcac ttgaaggaat cgaagccggt
     4741 gagcaagccc gatgaaaccg cctggttcag ctgatcgcac gtgacggtga tgccttgaaa
     4801 cttgagacag tcgctcggca acatcaactt gatggtcgtc gactggctga cgttggcctg
     4861 gtagctcatg tccgaattga aggtcaacga gccgctcacc ttggcgtcgg cgctctgaac
     4921 cgtcgccgtc gtacagccca acccactcat cagtgtgctc gcgatctgtg agcttgccgc
     4981 ggacgtatcg agacaagcgt ccttgatagc ccaagtaccg acgacgtcac ccccgcacgg
     5041 ctgcaccttc ccgcacgcgt aattcgaacc agatgaactt tctccacagc tcgctaaaga
     5101 caaccccaag acaccaagac tcacccaata cacacgtcgc accaaaacca tgtgacgatt
     5161 ctccctgcgc cgccacggaa ggcaagcgtt ttccgagtcg cagtcgagcc gagctccacg
     5221 gctctcgctc gggcggaggg agctcttgag ctgctcgaac ctggtacctt cgctgcgatg
     5281 agcctcgtcg acaagttcac gttcgagaag cgcggcgcgc gcggagtcag cgactcacat
     5341 tacgccttcg ccttg
//
LOCUS       DASTKS010000989         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_379364, whole genome shotgun sequence.
ACCESSION   DASTKS010000989 DASTKS010000000
VERSION     DASTKS010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379364"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFQ35_20920"
     CDS             <1..930
                     /locus_tag="VFQ35_20920"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933185.1"
                     /translation="KPGITFLASPGFLEHSVIAQRERAAHDAGDVLNADPRPAILEFA
                     SALARRGIALVLFPVPDKAMLQPRELHGRTERDRPARTARSRDWGRLLADLRQKGVLV
                     FDPTPEQIGPGEAPRFLVQDTHWTPAFMQTVAAELATYVERHVQLSMPAPGVAYTRKP
                     MRVSRVGDIVDMLKLPEGQTKFLPQTIVIPQVFDAAGEPFQPNAEAEVLLLGDSFSNI
                     FSEEFMGWGSAAGLPAQLATELRRPLDVIAQNDSGAFATRVALQLELERGNARLAGKK
                     VVIWEFASRELAVGNWKHIDWSKAMDTGNERTN"
     gene            914..1888
                     /locus_tag="VFQ35_20925"
     CDS             914..1888
                     /locus_tag="VFQ35_20925"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9933186.1"
                     /translation="MSARTETGQPGPVLVTGAAGVMGRRLVRGLLARGARVRGLTLPN
                     DPARAELAALGCEVVEGDLSEPDSLRDLTRGISTVYHLAAIILSHDPSVFQRVNLEGT
                     AHLVQAAAASSVEHFIYVSSASVVYPRRTPYAESKLAAEEVLRAQTGLHYTIVRPTLV
                     YDEHGGQEFVIFREFLLRYPVVPFVGSGLARKRPVFSQDVIDGLLLLLENPKSYGKLY
                     NFSGGEPITLIDLARLVLRHAGQQRSFVHVPVPLCRAVAKVLKVVMERPPITESAIAG
                     LVNHADLDPSEAIADLGYRPLGVHAGFERCFPIARAGLADERMKEMQS"
     gene            1885..>2417
                     /locus_tag="VFQ35_20930"
     CDS             1885..>2417
                     /locus_tag="VFQ35_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933187.1"
                     /translation="MKALVTLCSTLATAAAVIGLAACSAITGAPPKGSEDAIESDQAA
                     AMAAIGKKAPGTIVWSSSRNGNHDLFTMKTDGSGTKSITSGDQVDWFPRFSPDGSKIL
                     FTRSKQGWVSERDANDSEKWDTYVTNLDGSDITKLVENSSWASWVDSDTIVFVRGTKI
                     FKKKVPDGAEVQLMSSEG"
BASE COUNT          428 a          769 c          806 g          414 t
ORIGIN      
        1 aagcctggca tcaccttcct cgcgagcccc gggttcctgg agcacagcgt gatcgcgcag
       61 cgcgaacggg ccgcgcacga cgccggcgac gtgctgaacg ccgacccgcg cccagcgatc
      121 ctcgaatttg cgagcgcgct cgcgcgccgc ggcatcgcgc tcgtattgtt tccggttccg
      181 gacaaggcca tgctgcaacc gcgcgagctg cacggccgca ccgagcgcga tcgcccggcc
      241 cgcaccgcgc gcagcagaga ctgggggcgc ctgctcgccg atctgaggca gaagggcgtg
      301 ctcgtgttcg atccgacgcc ggagcaaatt gggcccgggg aggcgccccg cttcttggtg
      361 caggacacgc actggactcc cgcgttcatg caaacggtcg cggccgagct cgcgacctac
      421 gtcgagcgtc acgtgcagct ctcgatgccg gccccgggcg tcgcctacac tcgcaaaccg
      481 atgcgcgttt cgcgcgtggg cgacatcgtc gacatgctga agctgccgga aggacagacc
      541 aagtttttgc cgcaaacaat cgtcattcca caggtgttcg acgccgccgg cgagcctttt
      601 cagccgaacg ccgaggcgga ggtcttgctg ctcggggaca gcttcagcaa catcttcagc
      661 gaagagttca tggggtgggg cagcgcggcc ggtcttccgg cgcagctcgc gacggagctc
      721 cggcgcccat tggacgtcat cgcgcaaaac gattcgggcg cattcgcgac gcgcgtggcg
      781 ctgcaactcg agctcgaacg cggcaacgcg cggctcgccg gcaagaaggt cgtaatttgg
      841 gagtttgcct cgcgcgagtt ggcggtgggc aattggaagc acatcgattg gtcgaaagcg
      901 atggacacag gcaatgagcg cacgaactga gacgggccag ccagggccgg tgctggtgac
      961 gggcgccgcg ggcgtgatgg gccggcgctt ggtacgcggg ctgcttgctc gcggagcgcg
     1021 cgtgcgcggg ctgaccttgc cgaacgaccc cgcccgggcc gagcttgccg ccctggggtg
     1081 cgaagtggtc gagggtgact tgtcggagcc cgattcgctg cgcgacctca cgcgcgggat
     1141 ttcgaccgtc tatcacctcg ccgcgatcat tctctcgcac gacccgagcg tttttcagcg
     1201 cgtcaacctg gaggggactg cgcacctggt acaggccgca gcggcgagct ctgtcgagca
     1261 cttcatttac gtctcgtcag cttccgtggt ctatccgcgc cgcaccccct atgccgagtc
     1321 gaagctcgcg gcggaagagg tgctgcgcgc ccaaacgggc ttacactaca ccatcgtgcg
     1381 cccgacgctc gtgtacgacg agcacggcgg acaagagttc gtcatcttcc gcgagtttct
     1441 gctgcgctat cccgtggtcc cattcgtggg ctcgggactt gctcgaaagc gccccgtctt
     1501 cagccaggac gtgatcgacg gtcttttgct gctgctcgag aacccgaaga gctacggtaa
     1561 gctctacaac ttcagcggcg gcgagccgat cacgctgatc gatctcgcgc ggctcgtgct
     1621 gcggcacgca gggcaacagc ggagcttcgt tcacgttccg gtgccgcttt gtcgcgctgt
     1681 cgcgaaggtc ctgaaggtcg tgatggagcg accgccgatt acggagagtg ccatcgccgg
     1741 gctcgtgaac cacgcggatc tcgatccctc ggaagccatc gcggatctcg gctatcgccc
     1801 gctcggggtg cacgctgggt tcgagcgctg cttccctatc gctcgggcgg ggctggctga
     1861 cgaaagaatg aaggagatgc aatcatgaaa gcgctcgtga cgttgtgctc aaccctcgcg
     1921 actgcggcgg ccgtaatcgg actcgctgcg tgctcggcga tcactggggc tccgccgaag
     1981 ggttcggagg acgcgatcga gagcgatcag gccgccgcga tggccgcgat cggaaagaag
     2041 gcccccggca ccatcgtttg gtcgagctcg cgcaacggca accacgacct cttcacgatg
     2101 aagacggatg gttccgggac gaagagcatc acctcggggg accaagtcga ttggtttccg
     2161 cgcttctcgc ccgatggatc caagatcttg ttcactcgca gcaagcaagg gtgggtgagc
     2221 gagcgagacg cgaacgacag cgaaaagtgg gacacttacg tgaccaacct ggacggctcc
     2281 gacatcacca agctcgtcga gaactcgagc tgggcgagct gggtcgattc cgacacgatc
     2341 gtgttcgtgc gcggcaccaa aatcttcaag aagaaggttc ccgacggcgc agaagtgcag
     2401 ttgatgagca gcgaagg
//
LOCUS       DASTKS010000990         5364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_157116, whole genome shotgun sequence.
ACCESSION   DASTKS010000990 DASTKS010000000
VERSION     DASTKS010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157116"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFQ35_20935"
     CDS             complement(<1..287)
                     /locus_tag="VFQ35_20935"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9933188.1"
                     /translation="MKTTKLGVHGPEVSAIGLGSLAMGSAGPFGASDDDDAIRTIHEA
                     LERGVTLMDTADFYANGQNELLLARAIAGKRDKLVLSVKFGVMRGPNGQMLG"
     gene            355..1254
                     /locus_tag="VFQ35_20940"
     CDS             355..1254
                     /locus_tag="VFQ35_20940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9933189.1"
                     /translation="MEYQSEQALEELWAFVAVVEAQGFRAAARATGGRKATLSQRVQD
                     LEKRLGVPLLVRTTRSMRLTEEGRAYFEHASRALASARDAEAVVLSAKAAPRGVLRVA
                     VAAALASHVLDQVVARYLTKFPHVNVVLHKAERRVDLVRDGFDLVVWGGPLEDSSLVA
                     RRLGVASGGYFASPRYLAGRAVPQSPDDLAGHDLVTITKDRGSTEWTFVSGGKERRIP
                     IRPRLVVNDVEFAVRASIAGMGIAPAPIAIAEPHVAKKRLVQLLERWTRPGVDVYAIL
                     PPGALVPRTRAFLDLLQKYACAR"
     gene            complement(1463..1909)
                     /locus_tag="VFQ35_20945"
     CDS             complement(1463..1909)
                     /locus_tag="VFQ35_20945"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9933190.1"
                     /translation="MAENTVKLHRVLRAPAERIYRAFLDPDAMAKWLPPHGFTGKVHH
                     IKAEVGGTYKMSFTNLTTGHSHSFGGEFLELVPNERIRHTDKFDDPNLPGQMQTTITL
                     KKVSVGTEISIVQEGIPSVIPTEACYLGWQESLTLLTQLVEPEINQ"
     gene            complement(2042..3004)
                     /locus_tag="VFQ35_20950"
     CDS             complement(2042..3004)
                     /locus_tag="VFQ35_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9933191.1"
                     /translation="MRGPSSKLPQAGALHDALELLFERALRHIPKSRERTAQLASPRP
                     GLFLLRQHEATAFEASIYDPMLCLILQGCKEMAFAEHDFRLRAGECALVSHDLPIVSR
                     VREAPYLVLLLKLELDVLRSLYADLTDLPQASAEAHPLEVHRADPALLDALTRYLALA
                     DSKMDARVLGPMLQREVHYRLLTSPLGYMLRRLTRHDSHESAITRAIALLRKDFRSPV
                     VVEELARTVGMSVSSFHKHFKAVTSSSPLQYQKDLRLLEARRLLLAGSASVTTVAFDV
                     GYESPSQFSREYARKFGNPPSREPTLAPASGAILGRGGPRRERV"
     gene            3116..4219
                     /locus_tag="VFQ35_20955"
     CDS             3116..4219
                     /locus_tag="VFQ35_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9933192.1"
                     /translation="MTRLIYGATETRLESARVEGDALWLDKLELEQSTRWSWKSQGLC
                     RDDTCLPIPHDNDRPWVSGDRFDVAALWRYLEWPIVRSRAGDVWVLGECASRRAAPSS
                     SLSAPDFALPDLDGRLHRLSDYRGRKVFLVTWASWCGCRAELPKWQSLHEELSSHGFT
                     VLAVALDEPGAARPWIDAASPGYPCLVDRGHVVAELYNLVNVPEAIWIDEAGSMVRPP
                     EPAGMLDSFRAMDRATFALPAPARAERDRAKAAYLDAVRDWAIRGAGSQHALGAEAVI
                     ARLKAPAPETAEAHSRFRLGQALLRDGRADEAAVEFAEAIRLHPDSWAMWRQTADRNP
                     SGLAVSEAFWRRVDALGERHYYEPTDLTHPRVG"
     gene            complement(4282..4776)
                     /locus_tag="VFQ35_20960"
     CDS             complement(4282..4776)
                     /locus_tag="VFQ35_20960"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9933193.1"
                     /translation="MSAKAPQRRSTCPINASLELLGDRWSLLIVRDLVFAGLRTYKEF
                     LNGEEAIATNILADRLTSLQAAGLITSERDPDDGRKLVYRLTPKGFDLAPALLELGRW
                     AVKYEAGVRRPEPLRLWEADRDAFLGRLRKERLHDQPKAASPSGTDNEPAPDRPRRKL
                     GRTR"
     gene            4894..5361
                     /locus_tag="VFQ35_20965"
     CDS             4894..5361
                     /locus_tag="VFQ35_20965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9933194.1"
                     /translation="MDHVGVVVDDLERALAFFVELGMERAGGITPVQGRWVDRVVGLS
                     DVQVDIAFVRTPDGHSQLELTQFRHPAATTAEPNPPPNTLGLRRLMFLVEDIQEVIAR
                     LRPHGGELIGEVAEYEDIYRLCYLRGPGGIIVALAEEIREPLRAGGSHVTTGR"
BASE COUNT          918 a         1646 c         1798 g         1002 t
ORIGIN      
        1 ccgagcatct gcccgttcgg gccgcgcatc actccgaact tcaccgacag gacgagcttg
       61 tcccgcttgc cggcgatcgc gcgcgcgagg agcaactcgt tttgcccatt ggcatagaaa
      121 tctgcggtgt ccatcaaggt cacgccacgc tcgagcgcct cgtgaatcgt gcggattgcg
      181 tcgtcgtcgt ctgacgcgcc gaacgggccc gcactgccca tcgccaagct gccgagaccg
      241 atcgcggaca cttccggtcc gtgcactccg agtttggtgg ttttcattgt ccaaacagca
      301 tgcggtcgac cgaccgcgtt gataatcacc ccaattggga acagactggg ccgcatggag
      361 taccagtcgg aacaagcgct ggaggagctc tgggcgttcg tggcagtggt cgaggctcag
      421 gggtttcgcg ccgcggcgcg tgcgaccggt ggacggaagg ccacgctcag tcagcgcgtg
      481 caggacctcg agaagcgtct cggcgtgccg ctcctcgtgc gaacgacgcg ctcgatgcgg
      541 ctcaccgaag aggggcgcgc gtacttcgag cacgcttcgc gcgcgctcgc ttcggctcgc
      601 gatgccgagg cagtggttct ctcggctaaa gcggcgcctc gcggcgtcct gcgggttgcg
      661 gtggcagcgg cgctcgcatc ccacgtcctc gaccaagtcg tcgcacgcta cctcacgaag
      721 ttcccgcacg tgaacgtcgt tctccacaag gcggaaaggc gcgtcgatct cgtgcgcgac
      781 ggattcgacc tcgtggtctg gggcgggccg ctcgaggact catcgctcgt cgcgcggaga
      841 ctcggcgtcg catccggcgg gtactttgcg agcccgcggt acctagcggg acgggccgtg
      901 ccgcagtctc cggacgacct cgctgggcac gatctcgtca cgataacgaa ggaccgtggg
      961 tcgaccgagt ggacattcgt ttcgggaggg aaggagcggc gcattccgat tcgtccgcgc
     1021 ctcgtcgtaa acgacgtcga attcgccgtt cgcgcgagca ttgcgggtat ggggatcgcg
     1081 cctgctccga ttgcgatcgc cgagccgcac gtcgcgaaga agcgactcgt ccagttgctc
     1141 gagcgctgga cgcgccctgg ggtcgacgtc tacgcgattc ttccgccagg cgcgctcgtg
     1201 ccgaggacgc gcgcattcct ggacctgctg cagaaatacg cctgcgcgcg gtgacctgtg
     1261 atcaggcgac gcgccatcga ttcgttggaa cgagcgctcg gtggcagcac ctcggcactg
     1321 ttatggagcc cgcttcgcgg agaggggaga catacggcga cagtagtttg gtgctgccct
     1381 ttcgctagcc tgtcgccgtg ctcaccgctc gcgcctttgg cgagatggag ggcgcgagcg
     1441 ctcgagtcac gcgccccaaa acctactggt tgatttcggg ctcgacgagc tgggtcaaca
     1501 acgtcaaaga ctcttgccaa ccgaggtagc acgcctcggt ggggatcacg ctggggatgc
     1561 cttcctgcac gatgctgatc tcggtgccga cggacacctt cttgagcgtg atggtcgttt
     1621 gcatctggcc gggaaggttt gggtcatcga acttgtcggt gtggcgaatt cgctcgttgg
     1681 gaacgagctc gaggaattcg ccgccgaacg agtggctgtg ccccgtggtc aggttcgtga
     1741 acgacatctt gtaggtgcca ccgacttcgg ccttgatgtg gtgcaccttg ccggtgaagc
     1801 cgtgcggtgg cagccacttg gccatcgcgt cgggatcgag gaacgcgcga tagatccgct
     1861 cggcgggcgc acgaaggaca cggtgaagtt tgacggtgtt ttcggccatg gtgctttctc
     1921 ctttttggac ggtgattcgc gggcggaacg gctgctagcc atccgcatct acaacggcga
     1981 cgaacgagaa gagccgggat cgacacgcct cacaactttt tttctcggtg cgtccgtcgg
     2041 ctcagacccg ctcgcggcga ggtccgccac ggcctaggat ggccccagac gccggggcga
     2101 gggtcggttc tcggctcggc gggttgccga acttgcgcgc gtactctcgg ctgaactggc
     2161 tcgggctctc gtagccgacg tcgaaggcaa ccgtggttac cgaagccgag ccggcgagca
     2221 acaggcgccg cgcctcgaga agacggaggt ccttttgata ttggagtggg gacgacgacg
     2281 ttacggcttt gaagtgcttg tgaaaagacg agacgctcat gccgaccgtg cgcgcgagct
     2341 cttccacgac caccggggaa cggaagtctt ttcgcagcag cgcgatggcg cgagtgatcg
     2401 cgctctcgtg actgtcgtgg cgggtcaggc gtcgcaacat atacccgagc ggtgatgtca
     2461 ggagtcggta gtgaacctcc cgctgcaaca tcggcccgag cactcgcgcg tccatcttcg
     2521 agtccgcgag cgcaaggtag cgcgtcaggg catcgagcag agccggatcg gcgcgatgca
     2581 cctcgagcgg gtgcgcttcg gcgctggcct ggggcaggtc agtgagatct gcgtaaaggc
     2641 tccgaagcac gtcgagctct agtttgagca acaacacgag gtagggcgcc tcgcgaacgc
     2701 gcgaaacgat cggcaagtcg tggcttacga gcgcacactc tccggctcgt aagcgaaagt
     2761 catgttcggc gaacgccatc tccttacaac cttggagaat caagcaaagc atcgggtcat
     2821 agatgctggc ctcgaatgcg gtggcttcgt gctgccgcaa gaggaagagg cccgggcgag
     2881 gagaagcgag ttgcgccgtc cgctctctgg atttcggaat gtgccgcaga gcgcgctcga
     2941 acaggagctc gagcgcatcg tggagcgcgc ccgcttgcgg gagcttggat gaggggcctc
     3001 gcattcagtt gattctggta agttggatag aattgggcaa gtcacggcga gggttgggca
     3061 ggcgccgctc ttcttcgacc ggtagggtga cggacacctc acgaaggaga gcccgatgac
     3121 gagattgata tatggtgcca ccgaaactcg gttagagagc gcgcgcgttg aaggtgacgc
     3181 gctctggctc gacaagctcg agctcgagca atcgacacgc tggtcctgga agagccaggg
     3241 gctttgccgt gacgacacgt gcctgcccat tccgcacgat aacgaccgcc cttgggtgag
     3301 cggcgaccgt ttcgacgtgg cggctctttg gcgctacctc gagtggccta tcgtacgaag
     3361 ccgcgccggc gacgtttggg ttctcggtga atgtgcttcg cggcgcgctg ccccctcgag
     3421 ctcactgtca gccccggatt ttgcactgcc cgacctcgac ggacggctac atcggttgtc
     3481 ggactatcgg gggcggaagg tttttctggt cacttgggcg tcgtggtgtg gttgccgcgc
     3541 cgagctacca aagtggcagt ccctccacga agagctctcg tcgcacggat ttaccgtact
     3601 cgccgtggcc ctcgatgagc ccggcgctgc tcgcccctgg atcgatgcgg catcgcctgg
     3661 ctatccgtgc ctggtcgatc gaggtcatgt cgtagccgag ctttacaact tggttaacgt
     3721 gcccgaagcg atatggattg acgaagccgg gagcatggtc cgacctccag agccagccgg
     3781 catgctcgat agctttcgcg cgatggaccg cgcgacgttc gcgctgcccg cgccggctcg
     3841 cgccgagcgg gatcgcgcga aggccgcgta cctcgacgcg gttcgtgact gggccatccg
     3901 cggcgccggc agtcagcacg cgctcggtgc cgaagccgtc attgcacggt tgaaggcgcc
     3961 cgcaccggag acggcagagg cccattcacg cttccggttg ggtcaagcgc tgctccgcga
     4021 cggtcgcgcc gacgaagccg ccgtcgagtt cgccgaggcg attcgactcc atcccgactc
     4081 ctgggcgatg tggcgacaaa ccgccgatag gaatccgagc ggcctcgccg tgagtgaagc
     4141 gttctggcgc cgcgtcgacg cgctcgggga acgccattac tatgagccga ccgacctcac
     4201 ccacccgcgc gttgggtgag acattgtttc gcgctcctgc tgcgccgttg ccgcgccact
     4261 gcagccgttt gcgtgagtgg ctcagcgcgt ccgcccgagc ttacgccgcg gccgatcggg
     4321 ggcgggctca ttgtccgtgc ccgatgggct cgccgccttg ggctggtcgt gtaagcgctc
     4381 cttgcgcagc cgtcctagga aggcgtctcg atccgcctcc cagagccgca gcggttcggg
     4441 tctccgcaca cccgcttcgt acttgacggc ccatcgcccg agttcgagca gcgctggcgc
     4501 gagatcgaac cctttcggcg tcaatcgata gacgagcttg cgcccgtcgt cggggtcgcg
     4561 ctcgctcgtt atcaaaccgg ccgcttggag cgacgttaga cgatcggcga ggatattcgt
     4621 cgcgatcgct tcctctccat tcaaaaattc tttgtaggtt cggagcccgg cgaagacgag
     4681 gtcccgcacg atcaggagcg accagcgatc ccccaagagt tcgaggctcg cgttgatggg
     4741 gcaggtcgaa cgccgctgcg gcgccttggc tgacacgatg cccgaaatat atcacttgca
     4801 tcgtgcaagt aaagtcgcta gaccggacat gtcgcttgcg aattgcaagt gaaaggaggc
     4861 gacgtgccga aaagaacatc gaaaatcagg cggatggacc atgttggggt cgtggtggat
     4921 gatctcgagc gagcgctcgc gttcttcgtc gagctcggca tggagcgagc gggcgggata
     4981 acgccggtgc agggccgctg ggtcgatcgg gtggtgggtc taagcgacgt gcaagtcgac
     5041 atcgcgttcg tgcgcacacc cgacgggcac agccagctcg agctgaccca gtttcggcat
     5101 ccggccgcca ccaccgccga gccgaatccc ccaccgaaca cgctcgggct gcgtcggctc
     5161 atgttcctcg tggaagacat tcaggaggtc atcgcccgct tgcggccaca cggaggggag
     5221 ctgatcggtg aagtcgcaga gtacgaggac atctatcggc tctgctattt gcgcggcccg
     5281 ggtggcatca tcgtcgcgct ggccgaggag atccgagaac ccctgcgagc aggaggaagc
     5341 catgtcacga caggtcgatg atat
//
LOCUS       DASTKS010000991         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1274011, whole genome shotgun sequence.
ACCESSION   DASTKS010000991 DASTKS010000000
VERSION     DASTKS010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274011"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3306)
                     /locus_tag="VFQ35_20970"
     CDS             complement(<1..3306)
                     /locus_tag="VFQ35_20970"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HET9933195.1"
                     /translation="MLVVVAEAEATPQSFQEIVNAVRAAITLQHQLQVHAVVLIEPRS
                     LPKTSSGKVQRQATRAAYMAKTLNELTRDVVNNLGGAGRSTIRPPNTLELVRLAEREQ
                     GIALVTMVLIGDVARTLNVRRGQVDPTLPVNSLGLDSLLSVELTHKLERSLGVVFPVT
                     HFLRDTSLKDLATTLYDLIVQDEGPPVSSRLPRTTAHDDMLSRGQAALWLLHGFGAGA
                     AYNLASAFRVRSMVDAECLRLALEHLLERHEILRTTYQADDAGTPRVAARSAPLGFSV
                     VPAAELDESALRARVEQFAFTPFDLEQGPILRVGLFSRAAEDHVLVIAVHHVAADLWS
                     LVALMSELGKAYQAFKGKESPKLPVLETSYRAFVHWQEEVLSGPETYRSWSFWRSRLS
                     GGTPALELPTDRPRPPLRSHRGATVSVRFNEATSGRVRSLARALSVTPYSVLLATFQA
                     LLARYSGQTEFVVGTPVSGRARASFDNTIGYFVNVLPLVTRVDKGVTGRQLAHLAQAG
                     ALDALEHQDYPFVSLVERLAPARDPSRSPLFDVMFAFERPHLGPEQAVARLMLDSSAP
                     PVDWASLRLEGYELEEKTAQFDLVLMVADSDTHFSAKLTYNSDLFDAETGARLARHFS
                     ALLEALIESPEQDIWKLSFLSEAEQRALVSAGPRGDSLDDSIHGLIAARVERSPDAVA
                     AVFGDQAVSYRELDALASNVAHRLVQMGAGPGELVAICLERGIGMLVAMYAVLKCGAA
                     YIPIDPDYPRERQLFVLADARPKVLLTSRGRQKTFPDDDATVLCIEHALEGPNRDFRA
                     AKLGPGSMAYVIYTSGSTGKPKGVMVTHRGVVNFFAGMDRILRRTEGAWLALTSMSFD
                     ISVLELLWTLTRGLKVVIQRADGWSAAKRPSLELGLMLWGQDDAEHGGRYDFFLELVK
                     RADTAGFSSVWIPERHFHAFGGNYPNPVLLAAAAAVSTKRIAIRSGSVVLPLQNPVRV
                     AEEWAVVDNLSNGRVALAVASGWHKNDFVLAPENYAERGRVMLEGIDLVRHLWRGGST
                     RLPNGRGETTEVKTLPRPIQKELPIWLTAAGNTETFRTAGERGFNLLTHLLGQDIPTL
                     KEKIAVYREARRKA"
BASE COUNT          597 a         1108 c         1083 g          541 t
ORIGIN      
        1 cgcctttcgt cgcgcctccc ggtacaccgc gatcttttcc ttcaaagtcg ggatgtcttg
       61 cccgagcagg tgcgtgagga gattgaagcc gcgctcgccc gcggtgcgaa aggtttcggt
      121 gttgccggcc gcggtgagcc aaatcggaag ctctttttgg atcggccgcg ggagagtttt
      181 cacctcggtc gtctcgccgc gcccgttcgg aaggcgcgtg cttccgcctc gccacaagtg
      241 ccgaacgaga tcgatgccct cgagcatcac gcgtccgcgc tctgcgtagt tctccggcgc
      301 gagcacgaag tcgttcttgt gccagcccga agcgacggcg agcgcgacgc gaccgttcga
      361 gagattgtcg acgacggccc actcctcggc gacgcgaacc ggattctgca gcgggagcac
      421 cacgctgccc gatcgaatgg cgatgcgctt cgtggacacg gccgctgccg ccgcgaggag
      481 cactggattc ggatagttgc caccgaacgc atggaagtgc cgctccggga tccaaacgga
      541 cgagaaaccc gccgtgtcgg cgcgtttgac gagctcgagg aagaagtcgt accgacctcc
      601 gtgttcggcg tcgtcttggc cccacagcat gagcccgagc tcgaggctcg gccgcttggc
      661 ggcgctccaa ccatctgctc gctggatgac taccttcaac ccgcgcgtca acgtccagag
      721 cagctcgagc acggagatgt cgaaggacat gctcgtgagg gcgagccaag cgccctccgt
      781 gcgacgcagg attcgatcca tacccgcgaa gaaattcacg acgccgcgat gagtcaccat
      841 cacgcccttg ggcttgcccg tcgagcccga cgtgtagatg acgtacgcca tgctccccgg
      901 cccgagcttc gccgcgcgga aatcacggtt cggtccttcg agcgcgtgct cgatgcacaa
      961 caccgtcgcg tcgtcgtcgg ggaaggtctt ttggcgacct ctcgaagtca gcaacacctt
     1021 cggtcgagcg tcggcgagga cgaagagctg acgttcacgc ggatagtcgg gatcgatcgg
     1081 aatgtaggca gcgccgcact tgagcaccgc gtacatggcg acgagcatcc caatgccgcg
     1141 ctcgaggcaa atcgcaacga gctcacccgg ccccgcgccc atctgcacca gccgatgagc
     1201 gacgttgctc gccaaggcgt cgagttcacg gtagctcacg gcctggtcgc cgaacacggc
     1261 tgccaccgcg tcgggcgagc gctcgactcg cgcggcgatg agaccgtgga tcgagtcgtc
     1321 gagtgaatca ccgcgcggac ctgcgctgac gagcgctcgt tgctcggcct cgctgagaaa
     1381 cgacagcttc caaatgtcct gctcggggct ctcgatgagg gcctcgagta gcgcgctgaa
     1441 gtgccgcgcg aggcgcgccc ccgtctccgc gtcgaacaag tcggagttat aggtgagctt
     1501 cgccgaaaag tgcgtatcgc tatcggcgac catcaaaacc agatcgaact gcgcggtctt
     1561 ttcctcgagc tcgtagcctt cgagccgcag gctggcccag tcgacgggcg gcgcggagga
     1621 gtcgagcatg agccgcgcga cggcttgctc cgggccgaga tgcggccgct cgaatgcgaa
     1681 catcacgtcg aacaacggcg aacggctcgg atcgcgcgct ggcgccaacc gctcgaccag
     1741 gcttacgaag ggatagtcct ggtgctcgag cgcgtccaag gcgcccgcct gcgcgaggtg
     1801 agcgagctgg cgcccagtta cccccttatc gacccgcgtg acgagcggga gcacgttcac
     1861 gaagtagcca atggtgttgt cgaagctagc gcgcgccctg ccgctcaccg gcgtgcccac
     1921 gacgaactcg gtttgcccgg agtaccgcgc cagcaacgcc tgaaacgtcg cgagcagcac
     1981 gctgtacgga gtcaccgaga gcgcccgcgc gagtgagcga acgcggcccg acgttgcctc
     2041 attgaagcgc accgacacgg tcgcgccccg atgcgagcgt aacggaggcc ggggtcggtc
     2101 cgtcggcaac tcgagcgcag gcgttcctcc ggagaggcgc gaacgccaaa acgaccaact
     2161 ccggtacgtc tcggggccgc tcagcacctc ctcttgccag tgaacgaacg ctcggtagct
     2221 tgtctcgagc accggcagct tcgggctctc cttgcccttg aaggcctgat acgccttgcc
     2281 gagctcgctc atcaatgcga cgagcgacca gagatccgcc gcgacgtggt gaacggcgat
     2341 gaccaaaacg tggtcttcgg cagcgcggct gaacagccca acgcgcaaaa tggggccctg
     2401 ctccaaatcg aacggcgtga acgcaaactg ctcgacccga gctcgcagcg cgctctcgtc
     2461 gagctcggcg gcagggacga cgctgaaccc gagcggggcg ctgcgggccg cgacgcgagg
     2521 cgtgcccgcg tcgtccgcct ggtatgtagt gcgcaggatc tcgtgccgct cgagcagatg
     2581 ttcgagcgcg agccgcaggc attcggcgtc gaccatcgag cgaacgcgaa acgccgacgc
     2641 gaggttgtat gccgcgcccg cgccaaagcc gtgcaaaagc cacagcgcgg cctgcccgcg
     2701 tgacagcatg tcgtcgtgcg cggtcgtgcg cggcagccgg ctcgacaccg gcggcccctc
     2761 gtcttgaacg atgaggtcgt acagggtcgt ggcgagatcc ttcaagctcg tatcccgcaa
     2821 aaagtgcgtt acggggaaga ccacgccgag gctccgctcg agcttgtgcg tcagctccac
     2881 gctgagcagc gagtcgagcc cgagcgaatt gacgggcaac gttggatcga cctggccgcg
     2941 ccggacgttc aacgtgcgcg cgacatcgcc gatgaggacc atcgtgacga gcgcaatgcc
     3001 ctgctcacgt tccgcgagcc gcacgagctc gagcgtgttg ggcgggcgga tcgtggaccg
     3061 gcctgcgccc ccgaggttgt tcaccacgtc acgcgtgagc tcattcaagg tcttcgccat
     3121 gtaggcggca cgcgtagctt ggcgttgcac cttgccgctc gaggtcttcg gcaagcttcg
     3181 cggctcgatc agaaccacgg cgtgtacctg caactggtgc tgcaacgtga tcgccgcacg
     3241 cacggcgttc acgatctcct ggaaagactg cggcgtcgcc tcggcctcgg ccacgacgac
     3301 gagcatctcg tcggcgtcgt acttgaacg
//
LOCUS       DASTKS010000992         4923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1498528, whole genome shotgun sequence.
ACCESSION   DASTKS010000992 DASTKS010000000
VERSION     DASTKS010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498528"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /gene="rpsI"
                     /locus_tag="VFQ35_20975"
     CDS             complement(<1..244)
                     /gene="rpsI"
                     /locus_tag="VFQ35_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9933196.1"
                     /translation="MATATDRTYSTGRRKTAIARVWLAPGDGRIVINDRGDEDYFKRP
                     TARMVIRQPLEEVSLLGQYDVIATVRGGGISSQAGAI"
     gene            complement(431..1834)
                     /locus_tag="VFQ35_20980"
     CDS             complement(431..1834)
                     /locus_tag="VFQ35_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9933197.1"
                     /translation="MPKILIVDDQSAVRTALEVLFQVHGLETVAASTPAQALNLVASE
                     DIGVVVQDMNFSKDNTSGREGTELFRAIRKLDPELPVLLMTAWTSLETAVELVKEGAV
                     DYLAKPWDDQKLVVTVQNLLRIHDLGRENQRLRGRSNRARGELASKYDLCGLVYESAE
                     IHSLVELAIHVAASDAPVLITGPNGAGKERLAQIIQANSRRSDKPFIKVNVGALPDNL
                     LEAELFGAEAGAFTGATKLRVGRFEAAHGGTLFLDEIGNLSPSGQMRLLRVLQTGEFE
                     RLGSSTTRRVDVRVVSATNADLKRAIREGRFREDLFFRLNVIELMLPALVDRMDDVLP
                     LARHFLSELAPDKALTLDASAERALLEYDWPGNVRELQNRMQRAQLVCRGGVISAADL
                     ALPAAEPRASRSSGTGNAKADSDESEREQIEAALARAQGVVSRAAAELGLSRQALYRR
                     MDRLGMVVERRVRGAES"
     gene            1936..2751
                     /locus_tag="VFQ35_20985"
     CDS             1936..2751
                     /locus_tag="VFQ35_20985"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9933198.1"
                     /translation="MESWQVSAVVASCVLLPVGLAVATSPEIRRALRGTGSVRQTLSN
                     AGVGVLFLVTQVGLRGALIGAYAGAAALVPWKLPLGRPESWLFAFFFLELIYYVQHRL
                     EHRIPLLWAVHVVHHQSRDYNLSVSLRVGVLSSLTTTLFHLPLALLGVSLEQYALLST
                     IHAGLLSTLHARTRLALGPGRFFNAPVFHRVHHASDSSYIDKNFGGVLLVFDRLFGTF
                     TPLANEPTYGVAAQASPLNPLDANVAPWRALRAQVRELPTLRAKLKALFVDPR"
     gene            complement(2758..3462)
                     /locus_tag="VFQ35_20990"
     CDS             complement(2758..3462)
                     /locus_tag="VFQ35_20990"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9933199.1"
                     /translation="MGFNSDGGPRWDSPILFDTAARDAFRAGSYDARRRFDLASNITV
                     YGGMAWAAFDTFGVALGFDKNPRVAREMFWMDAEAYAVSLLLTNVTKRVALRSRPYAR
                     PCLADSGYDPHCGSFEANVSFYSSHSAMAGTTAGLICFQHQHLHLYGRGGDIASCVGA
                     LGLMTATGLFRMASDNHWASDVAVGYALGFASGYGLPYLLHFSPQASTRRSEVSVRVL
                     PYGDGSRVGLWALGSF"
     gene            3825..>4923
                     /locus_tag="VFQ35_20995"
     CDS             3825..>4923
                     /locus_tag="VFQ35_20995"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9933200.1"
                     /translation="MTRTLAFSSGLRSLNVPRLLAPLSCVAALAVTAETRADDLAYSK
                     LDDATVRVFCFKTVGLTRVKSRSGEAYALGAPDAGHGSGVIINKDGLILTARHVVEDA
                     RWVAVQLPGRDSPLPAQVVYQDKDHDTAFLVTNVDTPQYLELPAQKPKLAVRETVYVV
                     GYPLDASRSRPQSEQGIVSGVLPDGSLQLGIALNPGNSGGPVVDAKERFIGIAVARAD
                     PTKGAQGIGVAVPVDHILPAYSAVLKSSEYKSARGAKATSRDQARAELLMTLLTADDS
                     NSAWQALGGKGEAPPSSPSVDKVIEQLDKSGAQDADTLALAAAQEWNAAAVLQARKQS
                     SGARLEAARKLAKRAADADSKISERSPFVAFV"
BASE COUNT          817 a         1684 c         1580 g          842 t
ORIGIN      
        1 ggatggcgcc ggcctgcgac gagatcccgc caccgcgcac ggtggcgatc acgtcgtact
       61 ggccgagcag cgagacctcc tcgagcggct gccggatcac catccgggcg gtcggccgct
      121 tgaagtagtc ctcgtcgccg cgatcgttga tgacgatcct gccgtcgccg ggggcgagcc
      181 acacgcgagc aatggcggtc ttgcggcggc cggtggagta ggttctgtcg gttgcagtcg
      241 ccatgcgtcc tcagttcgcg tcggtcgcgt cggtcacgtc ggtcactggg cctcgagcac
      301 gcgcggctgc tgcgcctggt gctcgtgctg cggccccggg tagaccttga gcttcttgat
      361 gatccggcgc ccgagcgggc ccttggggag catgccccag accgcctcgc ggatggcgcg
      421 cgcggcaaag tcacgactcg gctcctcgca cccgcctctc gacgaccatg ccgagccggt
      481 ccatgcgccg atacaacgcc tgacggctga gcccgagctc cgcagccgcg cgcgagacga
      541 cgccttgcgc ccgcgcgagc gccgcctcga tttgttcgcg ctcgctctcg tccgagtcgg
      601 ctttggcgtt gccggtgccg ctcgaacgcg acgctcgggg ctcggccgcg ggcagcgcga
      661 gatcagccgc gctgatgaca ccgccgcggc agaccagctg agcgcgctgc atacggtttt
      721 gcagctcccg cacgttgccg ggccaatcgt actcgagcaa cgcgcgctcg gcgctcgcgt
      781 cgagcgtgag cgccttgtcc ggcgcaagct cgctcaggaa atgccgggcg agcggcagca
      841 cgtcgtccat gcggtcgacg agcgcaggca gcatcagctc gatgacgttg agccgaaaga
      901 atagatcttc gcggaagcgt ccctcacgaa tggctcgctt caagtcggcg ttggtcgcac
      961 tgacgacgcg gacgtcgact ctacgcgtcg tgctgctgcc gagtcgctcg aactcccccg
     1021 tttggaggac gcgcaagagc cgcatttgcc cgctcggcga gaggttcccg atttcatcca
     1081 ggaacaacgt gcccccgtgc gcagcctcga agcgcccgac ccggagcttg gtcgcaccgg
     1141 tgaaggcccc cgcctccgcg ccgaagagct ccgcctcgag caggttgtcg ggcagcgcac
     1201 cgacgttgac tttgatgaag ggcttgtcgc tccttcgtga gttcgcttgg atgatctgcg
     1261 cgaggcgctc tttgccggcg ccgttcgggc cggtgatgag tacgggtgcg tccgaggccg
     1321 cgacgtgaat cgcgagctcg accaaggagt ggatttcggc gctctcgtac acgagcccgc
     1381 agaggtcgta cttcgaagcg agctcgccgc gcgctcggtt gctgcggccg cgaagacgtt
     1441 ggttttcgcg gccgaggtcg tgaattcgca gcaaattctg cacggtgacg accagctttt
     1501 ggtcgtccca gggctttgcg aggtagtcga ccgcgccctc cttgaccagc tccaccgccg
     1561 tctcgagcga ggtccaggca gtcatcagga gcacgggcag ctccggatcg agcttgcgaa
     1621 tcgcgcggaa caactcggtt ccctcccggc ccgaagtgtt gtccttgctg aaattcatgt
     1681 cctgaacgac gaccccgata tcctcgctcg ccacgaggtt cagcgcctgc gccggagtgc
     1741 tcgccgcgac cgtctcgagc ccgtgcacct ggaacagaac ctcgagcgcg gtgcggacgg
     1801 cgctctgatc atcgacaatc aggatcttgg gcatgggctt ccgaacggtt accgtgtggg
     1861 tgcgctccgt caagaccgtt ccggtttgtg ctcgggtacg attcgcgtca ttttcctgct
     1921 tcggactcgc gcagaatgga atcctggcag gtttcagccg tcgtcgcgtc gtgtgtgctc
     1981 ttgccggtgg gcctcgccgt ggcgacctca cccgagatcc gccgcgcctt acgcgggacc
     2041 ggcagcgttc ggcagaccct ctccaacgcg ggcgtcggtg ttctgttcct ggtcacgcaa
     2101 gtgggcctcc gcggcgctct catcggcgcc tacgcgggcg ccgccgcgct cgtgccgtgg
     2161 aagctgccgc tcggccgccc cgagagctgg ctcttcgcct tcttcttcct ggagctcatc
     2221 tactacgtgc agcaccgcct cgagcaccgc attccgttgc tttgggccgt gcacgtcgtg
     2281 caccaccagt cgcgcgacta caacttgtcg gtgtcgctcc gagtgggcgt actctcgtcg
     2341 ctcacgacga cgctgttcca cctgccgctc gcgctcctcg gtgtatcgct cgaacagtac
     2401 gcgctgctct cgacgatcca cgccgggctc ttgtccacgc tgcacgcgcg cacacggctc
     2461 gctttgggcc ccgggcgctt cttcaacgcc ccggtgttcc atcgagtgca ccacgcgagc
     2521 gattcttctt acatcgacaa gaacttcggc ggcgtgctcc tcgtcttcga tcgcctgttc
     2581 ggcactttca cgccgctcgc gaacgagccg acttacggcg tcgcagccca agcgtctccg
     2641 ctcaatccgc tcgacgcgaa cgtcgccccc tggcgcgcgc tacgcgcaca ggttcgcgaa
     2701 cttccgacgt tgcgagcgaa gttgaaagcg ctgttcgtcg acccgcgttg atcgcgctca
     2761 aaaagagccg agcgcccaga ggccaactcg agagccgtca ccatagggca agacgcgaac
     2821 cgagacctcg ctccgccgag tcgacgcctg cggcgagaaa tgcaggaggt acggcaaccc
     2881 gtagccgctc gcgaacccga gcgcgtagcc cacggccacg tcgctcgccc aatggttgtc
     2941 cgacgccatg cgaaagagcc ctgtcgccgt catcaatcct agagcgccca cgcacgacgc
     3001 gatgtcgccc ccgcgtccgt aaaggtgcaa gtgctgatgc tgaaagcaga tgagccccgc
     3061 ggtcgtgccc gccatggccg aatggctgct gtaaaacgaa acgttggctt cgaaggaccc
     3121 gcagtggggg tcgtatcccg agtcggcgag acacggtcgc gcgtagggac ggctccgcag
     3181 agcgacgcgc ttggtgacgt tggtgaggag cagcgaaacg gcgtacgcct cggcgtccat
     3241 ccaaaacatc tcgcgcgcga cgcgcggatt cttgtcgaag ccgagggcca cgccaaaggt
     3301 gtcgaatgcg gcccacgcca tgccaccgta aacggtgatg ttgctggcaa ggtcgaacct
     3361 gcgccgagcg tcgtagctgc cagcgcgaaa ggcatcacgc gccgcggtat cgaagaggat
     3421 cgggctgtcc cacctcggcc caccatcgct attgaagccc atcaccgaaa cgacagcgcc
     3481 ggcaaaccca gtgagcgtta atgcatagtc cgctccgttg gattcccgga acttcgccgg
     3541 caactcgatg cgatgtgtcg gctcttgcgc gaaactgcgt gcgcacaccg cccaaccggc
     3601 caggccgagc aagacagctg gaaacaacgg ggctcgcacc aactcgatgc taaggacgcg
     3661 gcgcgcgttc ggcaaggcgg ctgctcgcac ctccagtgac cgacgcctta cgtttttcgc
     3721 gaggacgggc ccgagaaggg tcgctcgcga cacgagcgtc gtgataaaga cgctcaattg
     3781 gtgggcccgt gggaaaaaag cagttcgcgg gcgcatacgc agtcatgact cgaacgcttg
     3841 ccttttcttc gggcctccgc tctctcaacg tcccgcgcct gttagctccg ctttcgtgtg
     3901 tcgcggcgtt ggccgtaacc gccgagactc gcgccgacga cctcgcttac tcgaagctcg
     3961 acgacgcgac ggttcgggtc ttctgcttca agacggtagg cctcacgcgg gtcaagagcc
     4021 gctccggcga agcctacgcg ctcggggcac ccgacgcggg gcacggcagc ggggtgatca
     4081 tcaacaagga cgggctgatt ctgaccgctc gtcacgtcgt cgaggacgca cgctgggttg
     4141 cggttcagct gccagggcgg gattccccgc tccccgcgca ggtggtttac caggacaagg
     4201 accacgacac ggcgttcctc gtgacgaacg tcgacacgcc ccagtatttg gagctaccag
     4261 cgcaaaagcc gaagcttgcc gtgcgcgaga ccgtctacgt cgtcggctac ccgctcgacg
     4321 cgtcgcgttc gcgcccccaa tcggagcagg gcatcgtctc cggcgtgctg cccgacggct
     4381 cgcttcagct cggcatcgcg ctgaacccag gcaactcggg cgggccggtg gtggacgcca
     4441 aggagcgctt catcggaatt gcagtcgcgc gcgccgatcc aaccaaaggc gcccagggca
     4501 tcggtgtcgc cgtgccggtc gaccacattt tgccggccta ctccgcagtg ctgaagagca
     4561 gcgagtacaa gagcgcgcgc ggcgccaagg cgacgagccg cgaccaggcg cgcgcagagc
     4621 tcttgatgac gttgctcacg gccgacgact ccaacagcgc gtggcaggcc ctgggcggta
     4681 agggcgaagc gcccccgtcg agcccgagtg tcgacaaggt catcgaacag ctcgacaaga
     4741 gcggcgcgca agacgccgac acgctcgccc tcgcggccgc ccaagagtgg aacgcggcgg
     4801 cggtgctaca ggcccgcaag caatcgagcg gagcgcgcct cgaggcagcc cgaaagctcg
     4861 caaagcgcgc agccgacgcc gattctaaaa tcagcgagcg ctcaccgttc gtcgcgttcg
     4921 tac
//
LOCUS       DASTKS010000993         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1498667, whole genome shotgun sequence.
ACCESSION   DASTKS010000993 DASTKS010000000
VERSION     DASTKS010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498667"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2039)
                     /locus_tag="VFQ35_21000"
     CDS             complement(<1..>2039)
                     /locus_tag="VFQ35_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9933201.1"
                     /translation="AVKEHLILGALFAGAVVLLFLGSVRSTIIAAIAIPVSIVGTFAL
                     MWVEGFSLDTITLLALALAVGIVIDDAIVVLENIHRFIEEKKYSPVKAAVLATREIGL
                     AVLATTISLAAVFLPVAFMTGIVGRFLKSFGLTMAFSILVSLIVSFTLTPMLASRWLR
                     SSELHEGKAPKKSALERLVDSFYLPIERAYVALLAWVMRHRWVVVVASFASLAAVPPL
                     MKIVPKGFLPKSDEAHFEVNVRAPEGTSLEATSIVAERIARELRTRSDVQSTLLTIGD
                     SNDKRPNLARIYVRLTDPEKRKLSEDEIMDEVRRQVLPKQPKELALDVSQVAMFSGGG
                     FRTALIQYEISGPDLNKLADYSDKLVERIKKVPGAVDVASTYVGGKPELALRIDRKRA
                     AELGVNVADVAQTLRLFVGGVDVSSYVEKGETYPIRVRGEARYRTDLAGLSLLSVPSQ
                     KLGTVPLLEVVSSEPGTGPASIERANRRRQVLLLANVAPGYSQGEIAAAVEREVKALG
                     MPSEYTAAAFGQTREMARTGKAFAVAFLLSFVFMYLVLAAQFESWLHPITILLALPLT
                     LPFALVSLLIFKQQLDIYSMLGLLVLFGVVKKNSILQIDHMNHLRKEGRDRLTAILEA
                     NRDRLRPILMTSFAFVAGMIPLLVSTGIGAGFNRATAGVIVGGQILSLLLTLLAT"
BASE COUNT          380 a          634 c          687 g          338 t
ORIGIN      
        1 ggtggcgagc agtgtcagca gcagcgaaag gatttgcccg cccacgatca cgccggcggt
       61 ggcgcgattg aagccggccc cgatgccggt cgaaacgagc aggggaatca tgcccgcgac
      121 gaacgcgaag gaggtcatca gaatcggccg caagcggtcg cggttcgctt ccaggatcgc
      181 ggtgaggcgg tctcggcctt ccttgcgcag gtggttcatg tggtcgattt gcagaatcga
      241 gttcttcttg accacaccga acaagaccag gagccctagc atcgagtaaa tgtcgagctg
      301 ctgcttgaag atgagcagcg agacgagcgc gaacggcagc gtcagcggca gcgcgagcag
      361 gatggtgatg gggtggagcc acgactcgaa ctgcgccgcg agcacgaggt acatgaacac
      421 gaacgagaga aggaacgcca ccgcgaacgc cttgcccgtg cgcgccatct cgcgcgtttg
      481 tccgaatgcg gcggcggtgt actcgctcgg cataccgagc gccttgacct cacgctcgac
      541 cgcggccgcg atttcaccct gcgagtagcc gggcgccacg ttggcgagca ggagcacctg
      601 acgacggcgg ttcgcgcgct cgatcgaggc ggggccggtg ccgggctcgg agctcaccac
      661 ctcgagtaac ggcaccgtcc cgagcttctg gctcggcacg ctgagcagcg agaggcccgc
      721 gagatccgtc cgatagcgcg cttcgccacg cacacggatg ggatacgtct cgcctttctc
      781 gacatagctc gagacgtcca cgccacccac gaagagacgc agcgtttgag cgacgtcggc
      841 cacgttgacg ccgagctcgg ccgcgcgctt acggtcgatg cgcagagcga gctcgggctt
      901 gccgcccacg taggtgctcg ccacgtccac ggcgccgggg accttcttga tgcgctcgac
      961 gagcttgtcg gagtagtcgg cgagtttgtt caggtcgggt ccgctgattt cgtactgaat
     1021 cagcgcggtg cggaagccac cgccggagaa catcgcgacc tgcgacacgt cgagggcgag
     1081 ctctttgggt tgtttgggga gcacctggcg gcgcacctcg tccatgatct cgtcctcgga
     1141 gagcttgcgc ttttcgggat cggtcaggcg cacgtagatg cgcgcgaggt tcggtcgctt
     1201 gtcgttgctg tcgccgatcg tcaggagcgt cgattggacg tcgctgcgcg tgcgaagctc
     1261 gcgggcgatg cgttcggcga cgatgctcgt cgcctcgagg ctcgtgccct cgggagctcg
     1321 cacgttgacc tcgaagtgtg cctcgtcgct cttcggcaag aagcccttcg gaacgatctt
     1381 catcaggggc ggcaccgctg ccagcgacgc gaaggacgcg accacgacga cccaacgatg
     1441 cctcatcacc cacgcgagca gggcgacgta ggcgcgttcg atgggcaggt agaagctgtc
     1501 gaccaagcgt tcgagcgcgc tctttttcgg ggctttgccc tcgtgcagct ccgaagagcg
     1561 cagccagcgc gacgcgagca tgggcgtcaa cgtgaagctg acgatgagcg acacgaggat
     1621 cgagaacgcc atcgtgaggc cgaagctctt caagaagcga cccacgatgc cggtcatgaa
     1681 cgcgaccggg agaaacacgg ccgcgagtga aatcgtggtc gccagcacgg cgagaccaat
     1741 ctctcgcgtc gcgagcaccg ctgccttcac gggtgagtac ttcttctcct cgatgaagcg
     1801 gtggatgttt tcgagcacca cgatggcgtc gtcgatcacg atgccgacgg ccagggccaa
     1861 ggcgagcagc gtgatcgtgt cgagcgagaa gccttcgacc cacatcagcg cgaacgttcc
     1921 gacgatggat accggaatcg cgatcgcggc gatgatggtg ctgcgcacgc tgccgaggaa
     1981 caggagcacg accgctcccg cgaacagcgc ccccaagatg aggtgctcct tcaccgcgt
//
LOCUS       DASTKS010000994         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1498812, whole genome shotgun sequence.
ACCESSION   DASTKS010000994 DASTKS010000000
VERSION     DASTKS010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498812"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFQ35_21005"
     CDS             <1..203
                     /locus_tag="VFQ35_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933202.1"
                     /translation="ATGRNLGHPLALRSRGILVEAGRRTCPPSLDSIAIALAGRRAPI
                     VATASVGYVGFRYGVSPKRGSQ"
     gene            200..1345
                     /locus_tag="VFQ35_21010"
     CDS             200..1345
                     /locus_tag="VFQ35_21010"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9933203.1"
                     /translation="MTIARCKAKAALSMAVLVSACGRAEGGSTDADPSVGGSTTSANG
                     GHSGEAAASGGATSELQGGSSTTTGGSTASAGSGAGNAGECFPRSVPAVGDLAPPEVV
                     RDRLSAFLLEGPSSAPLPQTTTPSWAAELATQFVTESPGPNPAYRRLFANWFAMPGML
                     TRVTLERKAAIEERASAWGTTWSLALASNWDFRALLTQGPDAAPGTAGMLTDPAFLSV
                     YNTISWRGYWLTTSLLCINIPPEPAGPPSMQIFTREQLAEATPQPACSGCHRFFDPVG
                     FAFDQFDALGNYTGAPVDTSGRLVTPSGTTFEFSDIDALGRQLGSSCEVALCVANTFM
                     SQAWERTGVTPAQPNDEIPFVANQFAKSGFSGVELVRAIATTPSMLR"
     gene            complement(1368..2384)
                     /locus_tag="VFQ35_21015"
     CDS             complement(1368..2384)
                     /locus_tag="VFQ35_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933204.1"
                     /translation="MNDDELPSRPRVSLDELALFERLRERARRDRAPAHLASQILDRV
                     RDDARRERMLQSSNAAPERPRGVRRAVVLAGLLGVAASVALLAVHATAPPPISREAPA
                     RAAGSERASALAAGSERASAQTGAKPAPDPCAQGLFAAGSEPLIDDFEDGDDAVLANE
                     QRRGFWRWVRETDAPGTAPALLPIPRPVTGTGLLPGAALARAAGHNSLALHVAGGRLS
                     DWGAAIEFSFRPGCYDASRYAGIAFSARGPGRIYVAPREVSVIPIAEGGTCERECHNP
                     HVQKVDLDPQFKNYEVRFEEVRQRGIGKPPLDTKRLNSLAFLIRPEDTPYDVWLDDVR
                     FITR"
     gene            complement(2381..>2815)
                     /locus_tag="VFQ35_21020"
     CDS             complement(2381..>2815)
                     /locus_tag="VFQ35_21020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933205.1"
                     /translation="PADAEDAAQDVFWVFARRLNDVPLAAERAFLISTALHIAADKRR
                     TKWYSVSFELDVDARLSESPLPDEALDLRRAHAMLDSVLDGLPASERDVFVLSDIEEL
                     SRSEVARSLAISEGTVASRLARARERVEAALKRLCAKRGRRP"
BASE COUNT          451 a          977 c          944 g          443 t
ORIGIN      
        1 tcgcgacggg caggaacttg ggacaccccc tagcgcttcg cagccgcggg atcttggtcg
       61 aagcagggcg gcgaacttgc ccccctagcc tcgactcgat cgccatcgcg ctcgccgggc
      121 gccgggcgcc cattgtagcg actgcatcgg tcggatacgt tggcttccgt tacggtgtgt
      181 caccgaagcg gggaagccaa tgacaatcgc acgttgcaag gccaaggccg cactctcgat
      241 ggccgtcctc gtgagcgcgt gcggtcgcgc cgaggggggc tccaccgatg cggatccgtc
      301 tgtcggcggg agcacgacga gcgcgaacgg agggcatagc ggggaagcgg cggcgagcgg
      361 cggcgcgacg agcgagcttc agggcggctc ctccaccact accggcggct cgactgcgtc
      421 cgcgggctcc ggcgcaggaa acgccggaga gtgcttcccg aggtcagtgc cggcggtcgg
      481 cgacttggcg ccacccgaag tcgttcgcga tcgtttgagc gcgttcctcc tcgaagggcc
      541 ctcgagcgct cccctacccc aaacgaccac tccgagctgg gccgccgagc tcgcaaccca
      601 gttcgtgacg gagtcccccg gaccgaaccc tgcataccgg cgcctcttcg ccaattggtt
      661 tgccatgccg ggaatgctca cgcgcgtgac gctcgagcgt aaagccgcga tcgaggaacg
      721 ggcttccgcc tggggcacga cgtggtctct cgcgctcgca tccaactggg actttcgagc
      781 gctactgacg caaggtcccg acgccgcgcc gggtacggct gggatgctca ccgatccggc
      841 gtttctcagt gtctacaaca ccatctcttg gcggggttat tggctcacga cctcgctgct
      901 atgtatcaac atcccgccag agccagcggg cccaccttcc atgcagatct tcacgcgcga
      961 gcagctcgcc gaggccaccc cgcagcccgc gtgcagcggc tgccatcgct ttttcgaccc
     1021 ggttggcttc gctttcgacc agttcgacgc attgggtaac tacaccgggg cgccagtcga
     1081 tacgagcggt cgactcgtga caccgagcgg caccaccttc gagttctccg acatcgatgc
     1141 gctcggaagg caactcggct cctcctgtga ggtagctctc tgtgtcgcca acacgttcat
     1201 gtcgcaggcc tgggaacgca ccggggtcac accggcgcag cccaacgacg agattccgtt
     1261 cgtcgcgaat cagttcgcaa agtcgggctt ttccggtgtc gagctcgttc gggcaatcgc
     1321 gacgacgccg agcatgctcc gttgacgcgc gcgagcgctc gccgacttca gcgcgtgatg
     1381 aagcgcacgt cgtcgagcca tacgtcgtac ggcgtgtctt cggggcgaat caggaaggcg
     1441 aggctgttca ggcgtttggt atcgagcggc ggctttccga tgccgcgctg acgcacttct
     1501 tcgaagcgga cctcgtagtt cttgaattgc gggtcgagat ccaccttttg cacgtgcggg
     1561 ttatggcact ctcgctcgca cgtcccaccc tccgcgatcg gaatgacgga gacttctcgc
     1621 ggcgcaacgt agatgcgacc gggaccgcgc gcggagaagg cgatgcccgc gtagcgagaa
     1681 gcgtcgtagc aaccggggcg aaagctgaac tcgatcgccg cgccccagtc gctgagccga
     1741 ccgccggcga cgtggagcgc gagcgagttg tggcccgccg cgcgcgccag ggcggcgcca
     1801 ggcagtagcc ctgtgccggt cacgggacgc ggaatcggca agagcgcggg cgcggtgccg
     1861 ggcgcgtcgg tctcgcgcac ccagcgccag aagccgcggc gctgctcgtt cgccaagacg
     1921 gcgtcgtcgc cgtcttcgaa gtcgtcgatg aggggctcgc tgccggcggc gaacaaccct
     1981 tgcgcacacg gatccggtgc tggtttcgcc ccggtttgag ccgacgcccg ttcggagccg
     2041 gcagctagag cggacgcgcg ttcggagccg gcggcccgcg ccggcgcctc gcgcgaaatc
     2101 ggtggcggcg cggtcgcgtg caccgcgagc aacgcaaccg aagccgccac gccgagaagc
     2161 ccagcgagca ccaccgctcg gcgcacgccg cgcggtcgct ccggagccgc gttggacgat
     2221 tggagcatcc gctcacggcg cgcgtcgtcg cgcacgcgat cgaggatttg cgacgccagg
     2281 tgcgccggcg ctcgatctcg ccgagcgcgc tcgcgcagcc gctcgaacag cgcgagctcg
     2341 tcgaggctca cccgcggacg cgaaggcagc tcgtcgtcgt tcacggacgc ctcccgcgct
     2401 tcgcgcacag ccgcttcagc gcggcctcga cgcgctctcg agctcgcgcg agccgcgacg
     2461 cgaccgtccc ctccgagatc gcgagcgacc gcgctacttc cgagcgcgaa agctcctcga
     2521 tgtcggacaa gacgaagaca tcgcgttcgc tcgccggcag accatcgagc acgctgtcga
     2581 gcatggcgtg agcgcggcgc aggtcgagcg cctcgtcggg caacgggctc tccgacaatc
     2641 gggcgtccac gtcgagctcg aagctgaccg agtaccactt ggtgcgcctc ttgtcggcgg
     2701 cgatgtgaag cgcggtcgag atgaggaacg ctctctcggc cgcgagcgga acatcgttca
     2761 accggcgcgc aaacacccaa aacacgtctt gcgcggcgtc ctccgcgtcc gcggg
//
LOCUS       DASTKS010000995         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1945216, whole genome shotgun sequence.
ACCESSION   DASTKS010000995 DASTKS010000000
VERSION     DASTKS010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945216"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFQ35_21025"
     CDS             complement(<1..390)
                     /locus_tag="VFQ35_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933206.1"
                     /translation="MHLGAPLGRALGAAIMVTALGIGGCRTSNDDVHRWANTAQGPRK
                     LLAVLTHDKYPLELRVEAALSLISMKPRAGRRVGIQGSDDQPGVVDALGQLPPAARNA
                     IIGRLVPRLLEEMKRPLPVAQAGQPAPT"
     gene            642..>2587
                     /locus_tag="VFQ35_21030"
     CDS             642..>2587
                     /locus_tag="VFQ35_21030"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9933207.1"
                     /translation="MGARLFSPRSAEEIPQVPRASAPASSRLGHGPWREAASLLLFAG
                     AAFIALALASFKFDPDNASVHGSDWVGAAGTLVAHVLVQGFGVVAWLAPIELALIGRP
                     LIRGRMPENLGLRIAGDLVVAIVLAALCQVALPDALVFGHGGFAGNVGLLFGEMMREA
                     FSTVGSFLVGPTVVGLILIGRSSFSFIEWARRFAALMERLSARLAAAWQRLGGAWSTA
                     RALRKQEKLDQPAPRIVPRDPDAAIMIQLEDDDSDWIPVEETAAPPLAISEALRKAAS
                     TRSLALAEAETPVTRATKSVEAPAVDVAALTSTPPPDGTEKRKRNRGRRAKDKEPEAT
                     VEAAEPEVEAEASAEEEPELELEASAEPPEAAALPKKPRKRKSNEPEIVDTQHERAVE
                     KPAETVAPRVRDDFRLPSCELLEPVPVVQNAVSEELLKENGQLLMKTLLDYGVQASVQ
                     AILPGPTVTMYEVSCEAGTKVSKVAALADDLALGLSRKVRIIAPIPGKNRIGFELPNE
                     ERIPVSLRELVEDQRFDRLAEKAPLPVVLGRDIVGKPFYADLASMPHVIVAGATGAGK
                     SVGLNVMLSSLLFKRTPQELRMLMIDPKVVELAPFDGIPHMLLPVVTDMKQAATALRW
                     AVDEMERRYQLFANAGTKNISTYNG"
BASE COUNT          447 a          826 c          891 g          423 t
ORIGIN      
        1 cgtcggcgcg ggctgaccag cttgcgcgac tggaagcggc cgcttcattt cttcgagcag
       61 gcgcgggacg agcctgccga tgatggcatt gcgcgcggcg ggcggaagct ggcccagcgc
      121 gtcgacgacc ccaggctggt cgtcgctacc ctggatgccg acccgacgcc ccgcccgcgg
      181 tttcatactg atgagtgaga gagcagcttc tacgcggagc tctagcgggt acttgtcgtg
      241 cgtgagcacg gccaaaagtt tccgcggacc ctgggcggtg ttggcccagc ggtgaacgtc
      301 gtcgttgctc gttcggcatc cgccgatgcc cagcgccgtt accatgatgg ccgcgccgag
      361 cgctcggccc aagggagccc ccaagtgcat acgtgcttgc tccgttctcc tgcttcgccc
      421 gcgtgtgatt cacacgggag cggggttgag tggccatagt acagcccggt cgtattgaga
      481 ctcaagcggg ggaaccgccg gctcgaagaa ctggaagcgc gcgagctgcc agggaatttt
      541 ggcctgatga ccggtgtgcc cgctggccgc tccccagggc gatgaccgtg atctgtgaaa
      601 gttgataggc ccaagtgggc tgatgtaagt atatgtaacc aatgggcgca cgtctgttca
      661 gcccgcgttc cgcagaagag attccgcagg tcccccgagc cagcgcgcca gcgagctcgc
      721 ggctaggaca cggcccgtgg cgtgaagccg catcgctttt gctgtttgcg ggcgcggcct
      781 tcatcgcact cgcgctcgcg agcttcaagt tcgatccgga caacgcgagc gttcacgggt
      841 ccgattgggt aggcgcggcg gggacgctcg tcgcccacgt gttggtgcag ggcttcggcg
      901 tcgtcgcctg gctcgcgccg atcgagctcg cgctgattgg ccgtccgctc atccgcggac
      961 gcatgccgga gaatctcgga ctgcgcatcg cgggcgattt ggtcgtggcg atagtgctcg
     1021 ccgccttgtg tcaggtggcg ttgcccgacg cgctcgtgtt cgggcacgga ggattcgcgg
     1081 gaaacgtcgg gctgttgttc ggcgaaatga tgcgcgaggc gttctcgacc gtcggctcct
     1141 tcctggtggg gccaaccgtg gtcgggctca tcttgattgg gcgcagcagc ttctcgttca
     1201 tcgagtgggc gcggcgcttt gccgcgctca tggagcggct ctcggcacgg ctcgccgccg
     1261 cttggcagcg cttgggcggt gcgtggtcga cggcgcgcgc gctgcgtaag caagagaagc
     1321 tcgaccaacc cgcgccgcgc atcgtgccac gcgatcccga cgcggcgatc atgattcagc
     1381 tcgaggacga cgactccgac tggatccccg tcgaggagac ggccgcgccg ccgctcgcga
     1441 tttcggaggc gctgcgcaaa gcggcttcga cccgctcgct cgcgcttgcg gaagccgaaa
     1501 caccggtgac gcgcgcgacg aagtcggtcg aagcgccggc ggtagacgtc gcggcgctga
     1561 cctcgacccc accgcccgac ggaaccgaga agcgcaagcg caaccgtggc cgccgcgcga
     1621 aggacaaaga acccgaagcg acggtcgaag cggcggagcc cgaggtcgaa gccgaagcga
     1681 gcgccgaaga agagccggag ctcgagctcg aagcgagcgc cgagccgccg gaagcggcag
     1741 cgctaccgaa gaagccgcgc aagcgcaagt cgaacgagcc cgagatcgtc gacacgcagc
     1801 acgaacgggc cgtagagaaa cccgccgaga cggtggcgcc gcgggtgcgc gacgatttca
     1861 gactgccatc gtgcgagctc ttggagccgg tgccggtcgt gcaaaacgcg gtgagcgagg
     1921 agctcttgaa agagaacggg cagctcttga tgaagacgct gctcgactac ggcgtacaag
     1981 cgagcgtcca ggcgattttg ccgggaccca cggtgacgat gtacgaagtc tcgtgtgaag
     2041 ccggcaccaa agtgagcaag gtagccgcgc tcgccgacga tctcgcgctc ggcctttcgc
     2101 gcaaggtccg catcatcgcg ccgattccgg ggaaaaaccg gatcggcttc gagctcccca
     2161 acgaagagcg catccccgtg agtctgcgtg agctcgtcga agaccagcgc ttcgatcggc
     2221 tcgccgaaaa ggcgcctttg ccggtcgtgc tcgggcgcga catcgtcggc aagccgttct
     2281 acgccgacct ggcctcgatg ccgcacgtga tcgtcgcggg cgcaaccggc gccggtaaga
     2341 gcgtcggcct caacgtgatg ctctcgagcc tgctcttcaa gcgcactccg caagagcttc
     2401 ggatgctgat gatcgatccg aaggtcgtcg agcttgcgcc cttcgacggc attccgcaca
     2461 tgctcttgcc ggtcgtcacc gacatgaagc aggcggcgac ggcgctgcgc tgggccgtcg
     2521 acgagatgga gcggcgatac cagctgttcg caaacgccgg caccaagaac atcagcacgt
     2581 acaacgg
//
LOCUS       DASTKS010000996         5886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1050336, whole genome shotgun sequence.
ACCESSION   DASTKS010000996 DASTKS010000000
VERSION     DASTKS010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050336"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VFQ35_21035"
     CDS             <1..1276
                     /locus_tag="VFQ35_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933208.1"
                     /translation="AYELFVPFGEFKVKFGVDPERAQEFLAKELGVSVETLRAEASFR
                     RLAIRRRVPGEPPTIWEVTPANLRAAVIASGRYLARSVRDDGTFRYEVNGATGEDTPE
                     YNIPRHAGATWYLAQAAVYSRDRNMRRAVRLASNYLTTQQWADCGDHKCIADQEQADL
                     GSSALALLALVELVDGGISPELTPQVAALADFIRSQQRPDGEFKHLYNRATNTPIDIQ
                     FLYYSGEAAFALARAHRITHDPRDLEAASRALWQLVEKPWWYIGWRYYWPAEHWTCHA
                     LDELWDRAPNQKALRFCLDWQEFVRDTAIHGREAAPEYEAGATSGAFVPPQIIVSASR
                     MEAAVATLAAAEAAQIPKAELERLEAGIRGTLAFMMRFQFTPGPAHLMPDPEAMHGGF
                     PTSETDLRVRIDNPQHAGTGLLEYLRILKARR"
     gene            1383..2492
                     /locus_tag="VFQ35_21040"
     CDS             1383..2492
                     /locus_tag="VFQ35_21040"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HET9933209.1"
                     /translation="MFRKTAAELGSDPSGDAPLRFPSMANRDARRLALCAGVALALHA
                     ASALFHPRLKAARAALAESTDAGLLLSVEELAAEGSNAPGGGSPEPEPVEAKSAPEQR
                     VEPVHKRTRVVVRAAVPKEAPQANDQPDDTTQSELGPAPADLLASAEGSADEAPERPR
                     AARSFRLQNVRNESAASPDAPAQDESGSSSEGAGFGINGGPGGTGRGNGNGVNRRFAF
                     GGPKGAFHAEICFIEDTVKRLADITNCAPVGTFSTDAINVEPRRFNEGFPGVSTRTAW
                     FAIKYRGKFKVESADYYTFRLLSDDGAALRIDGYPIIDNDGQHMPRSVQATITLEAGE
                     HEFALFYYQGPPEFLALQLFVKQFNGSERLFGPVI"
     gene            complement(2547..4025)
                     /gene="pap"
                     /locus_tag="VFQ35_21045"
     CDS             complement(2547..4025)
                     /gene="pap"
                     /locus_tag="VFQ35_21045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HET9933210.1"
                     /translation="MFEAAETHQVLEKATYDAALPALRERLLKAQYALLEARKFPVVV
                     VIAGIPAAGRSETVRALSEWMDPRHLQVHAMGPPSDEELERPPMWRYWRRLPPKGKIG
                     VFFGSWYTQPIIAHALKKTGDRRFAESLEEVRRFERMLSDEGALVLKFWFHLTKKEQK
                     KRLDDLWHDKRTRYRVTKEDRKRAAHYEEFRDTSERALNATHAADTPWIVLEGYDDRY
                     RNFNAARALLEAIEGRLATERAAEPEPAAANVEPVTRRTNVLGSLDLTRALEKEAYAA
                     ELAKQQRRLAELTRKRAFARLSPVLVFEGMDAAGKGGAIRRVTQALDPRIYDVHAIAA
                     PSDEERAQPYLWRFWRHAPRDGKFAIFDRSWYGRVLVERVEGFAKVSEWKRAYEEIND
                     FERQLQEAGNVVVKFWLQISNEKQLARFQEREQTPFKRFKITQEDWRNREKWADYQRA
                     ASEMIERTSTQISPWTLVEAEDKYFARVKVIRTICEAIDAAT"
     gene            4139..4468
                     /locus_tag="VFQ35_21050"
     CDS             4139..4468
                     /locus_tag="VFQ35_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933211.1"
                     /translation="MKRKPRPTRERTLRRSDERAAQSLANDRERLFRLEAGGSPGRPI
                     EVGSASVIEAYALSMRCPRCDGAHELREHLAVTLEGARLREVRLVCRQCGSRRSLFFR
                     LRDDRPN"
     gene            complement(4515..5540)
                     /locus_tag="VFQ35_21055"
     CDS             complement(4515..5540)
                     /locus_tag="VFQ35_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933212.1"
                     /translation="MELSDPARLSLVVRRYGEALTALELEPGQQPLVLPTSEWFPDEF
                     KQDQESLERLIARMQGYAGLEKVDIEVALSGMPKAAGCGTGGCGSGACGTEKAPAEAP
                     QLSKTESGYSIAMPGSALSHPIAFTASIARMLGCIRLIEAGNEEPDAAISELAAVGLG
                     FGVLLLEASYLYNKSCGGPSVGTATALPLADLALPFALFLATEGHKPRRAMGELATTQ
                     RAVLDEACALASSNRTLVQRLREAPERVARGDFSLGETRSWFSRLFSSSKPRKVRDVE
                     SAALAALERGEDLDSVAALLQRDASAERTSKSPAAGRANDDVQSLVDEALAELRSERG
                     GGPSPAE"
     gene            5657..>5886
                     /locus_tag="VFQ35_21060"
     CDS             5657..>5886
                     /locus_tag="VFQ35_21060"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9933213.1"
                     /translation="MKRIGFIGLGTMGRGMVRNLVRKGYPTTVWNRSPATVSLLGVEV
                     SVAATPRALAEQCDVVIACVSDPPAVERVVFA"
BASE COUNT          927 a         1987 c         1927 g         1045 t
ORIGIN      
        1 cgcttacgag cttttcgttc ccttcggcga gttcaaggtc aagttcggcg tcgatcccga
       61 gcgcgcgcag gagttcctcg cgaaggagct cggggtgagc gtcgaaacgc tccgcgcgga
      121 ggcgagcttt cggcgcctcg cgattcggcg gcgcgtgccg ggcgagccgc cgacgatttg
      181 ggaagtgacg ccggcgaact tgcgcgcggc ggtgatcgca tcggggcgct acttggcccg
      241 cagcgtgcgt gacgacggca cgtttcgcta cgaggtcaac ggggcgaccg gcgaagacac
      301 ccccgagtac aacatcccaa ggcacgccgg cgcgacctgg tacctcgccc aagccgcggt
      361 ttacagccgc gatcgcaaca tgcgccgcgc ggtgcgcctc gcctccaact acttgaccac
      421 gcagcagtgg gccgactgcg gcgaccacaa gtgcatcgcg gatcaagagc aagccgatct
      481 cggatcgtcc gcgctcgcgc tgctcgcgct ggtcgagctg gtcgacggcg ggatttcacc
      541 ggagctcacg ccgcaggtcg cggccctggc cgacttcatt cgtagccagc agcgcccgga
      601 cggtgagttc aagcaccttt acaaccgcgc gacgaacacg ccgatcgaca tccagttcct
      661 ctactactcg ggcgaggcgg cgttcgcgct cgctcgcgcg catcgcatca cccacgaccc
      721 acgcgatctc gaggcggctt cgcgagcgct ctggcaactc gtcgaaaagc cgtggtggta
      781 catcggctgg cgctattact ggcccgctga gcactggact tgccacgcgc tcgacgagct
      841 ctgggatcgc gcgccgaacc aaaaggcttt gcgcttctgc ctcgattggc aagagttcgt
      901 gcgcgacacc gcgattcacg gacgcgaggc cgcccccgag tacgaagcgg gtgcgacgag
      961 cggcgccttc gtgccccccc aaatcatcgt ctccgcgagt cggatggaag cggcggtcgc
     1021 gacgctcgct gcggccgagg cggcgcaaat ccccaaagcc gagctcgagc gactcgaggc
     1081 cggcatccgc ggcacgctcg cgttcatgat gcgcttccaa ttcacgccgg gcccggcgca
     1141 cctgatgccc gatcccgaag cgatgcacgg cggcttcccg accagtgaaa ccgacctgcg
     1201 cgtgcgcatc gataaccccc agcacgccgg caccggcctg ctcgaatatc tgcggatttt
     1261 gaaggcgcgg cgctgattgc tgaatatatt cgagcgcttg gccaatgccg ggcggcctcg
     1321 gcggttcgcg ctttttctcg atcgaatcga tgtcacgctg acatccgatc tctgcgcgcg
     1381 ccatgttcag gaagaccgct gccgagcttg gttcggatcc ctcgggtgat gctcccttgc
     1441 ggtttccctc gatggcgaac cgagacgcgc gccggctcgc gctgtgcgct ggggtagctc
     1501 tagcgctgca cgcagcgagc gcattgttcc atccccgcct caaggccgcg cgcgccgcgc
     1561 tcgccgagtc aaccgacgcc ggactcttgc tgtcggtcga agagttggcc gctgaaggca
     1621 gcaacgcgcc cggtggtggc tcccccgagc cggagccggt tgaagccaag agcgctcccg
     1681 aacaacgcgt cgagccagtg cacaaacgga cgcgagtcgt cgtgcgcgcc gcggtaccga
     1741 aggaggcgcc gcaagcgaac gatcagcctg acgacacgac gcagtcggag ctcgggcccg
     1801 cgccggccga cttgctcgcg agcgccgaag gcagcgccga cgaagccccc gagcgcccgc
     1861 gcgccgcgcg ctcgtttcgc ttgcagaacg ttcgcaacga gtcggccgcg tcgcccgatg
     1921 ctccagcgca agacgagagt ggaagctcct ccgaaggcgc gggcttcggc atcaacggcg
     1981 gtcccggcgg caccgggcgc ggcaacggca acggcgtgaa ccggcgcttc gcgttcggtg
     2041 gtccgaaggg cgcttttcat gcggagattt gcttcatcga agacacggtg aagcggctcg
     2101 cggacatcac gaactgcgct ccggtcggga ccttctccac ggacgccatc aacgtcgagc
     2161 cacgccgctt caacgagggc tttccggggg tgagcacgcg cacggcgtgg tttgcgatca
     2221 agtaccgcgg caagttcaag gtcgagagcg ccgactacta cacgtttcgc ttgctctccg
     2281 acgacggcgc cgcgctgcgc atcgatggtt accccatcat cgacaacgac gggcagcaca
     2341 tgccgcgctc cgtgcaagcc acgatcacgc tcgaagcggg cgagcacgag ttcgctctct
     2401 tctattacca aggcccgccc gagtttttgg ctctccagct cttcgtgaag caattcaacg
     2461 ggagtgagcg cctcttcggt cccgtgattt gagcccgtgt ctcgagacgt cggccgcgcg
     2521 ggatgtcgag cgggcctagg tgtggcctag gtggcagcgt cgattgcttc gcagatcgtt
     2581 cggatgacct tcactcgcgc gaagtatttg tcctccgctt cgaccagggt ccacggcgaa
     2641 atctgcgtgc tggttcgctc gatcatctcg ctcgccgcgc gctgatagtc cgcccatttc
     2701 tcgcggttgc gccagtcttc ttgcgtgatc ttgaagcgct tgaacggcgt ctgctcgcgc
     2761 tcttgaaaac gcgcgagctg cttttcgttg ctgatctgca gccagaattt gacgacgacg
     2821 ttgccggcct cttgcagctg tcgctcgaag tcgttgatct cttcgtaggc gcgcttccat
     2881 tcgctgacct tcgcgaagcc ttcgacgcgc tcgacgagga cgcggccgta ccagctgcga
     2941 tcgaagatcg cgaatttgcc gtcgcgcggc gcgtgcctcc aaaaacgcca gaggtaaggc
     3001 tgcgcccgct cttcgtcgct gggcgcggcg atggcgtgca cgtcgtaaat gcgcggatcg
     3061 agcgcttgcg tcacgcggcg gatcgctccg cctttgcccg ccgcgtccat gccttcgaac
     3121 accagcaccg gcgagagccg cgcgaaggcg cgctttcggg tgagctcggc gaggcggcgt
     3181 tgctgcttcg cgagctcggc cgcgtaggct tccttttcga gagctcgcgt caaatccaag
     3241 ctgccgagga cgttggttcg ccgcgttacc ggctccacgt tggcggccgc gggctccggc
     3301 tccgcagcgc gctcggtcgc gaggcgcccc tcgatcgctt cgagcagggc ccgcgccgcg
     3361 ttgaagttgc ggtagcggtc gtcgtatccc tcgagaacga tccacggcgt gtcggcggca
     3421 tgggtcgcgt tgagcgcgcg ttcgctcgtg tcgcgaaact cttcgtagtg cgcggctcgt
     3481 ttccgatcct ccttcgtcac gcgatagcgc gttcgcttgt cgtgccagag gtcatcgagc
     3541 ctcttttttt gctccttctt ggtgaggtgg aaccaaaact tcaacaccag cgcgccctcg
     3601 tcgctgagca tccgctcgaa gcgacgcacc tcttcgaggc tctcggcaaa acgccgatca
     3661 cccgtcttct tgagcgcgtg ggcgatgatc ggctgggtgt accaggagcc gaagaagacc
     3721 ccgattttgc ccttcggggg gagccgtcgc cagtagcgcc acatgggcgg gcgctcgagc
     3781 tcttcgtcgc tcggcggtcc catcgcatgg acctgcaagt gccgtggatc catccactcg
     3841 ctcaaggcgc gaaccgtctc gctgcgaccc gcggctggaa tgcccgcgat gacgaccacg
     3901 accgggaact tgcgagcctc gagcagcgca tattgggcct tcaagagccg ctctcgcaag
     3961 gccggcagcg cggcgtcgta ggttgctttc tcgagcacct ggtgcgtctc ggccgcctcg
     4021 aacatgagcg catcctatcc caaaatccct gggcttcagc cggcttgcgt cagcgcgcgg
     4081 cgtcccgcat cctgagccga cggttcgcaa atgacttttt tgacccgcga gagcgcccgt
     4141 gaagcgtaaa ccgcgcccca ctcgagagcg gactctgcgc cgaagtgacg agcgagccgc
     4201 gcagagcctc gcgaacgatc gtgagcgctt atttcgactc gaggcggggg gaagcccggg
     4261 ccgaccgatc gaagtcggct ccgcgtcggt catcgaggcc tacgcactgt cgatgcgctg
     4321 tccgcgctgc gacggcgctc acgagctgcg cgagcacttg gcggtgacgc tcgagggagc
     4381 gcgcctgcgc gaggtacggc tcgtttgccg gcagtgcggc tcgcgccgct cgctcttctt
     4441 caggttgcgg gacgatcgcc cgaactgagg gcccgcaagg agccttacct tttcgggggc
     4501 cgtgagccgc tgagctattc ggcggggctc ggtccgcccc cgcgttcgga gcgcagttcg
     4561 gcgagagcct cgtcgacgag gctctgcacg tcgtcgttcg cgcggcctgc ggccgggctc
     4621 ttggaagttc tctccgccga tgcgtcgcgt tgcagcagcg ccgcgacact gtcgagatct
     4681 tcgccgcgtt cgagggctgc gagcgcggcg ctctcgacat cgcggacctt tcggggcttg
     4741 ctcgacgaga acaaccgcga gaaccacgag cgggtttctc cgagcgagaa gtcgccgcgc
     4801 gcgacgcgct ccggcgcctc gcgcaggcgt tgtacgaggg tgcggttcga gctggccagc
     4861 gcacaagctt catcgagcac ggcgcgttgc gtggtcgcga gctcgcccat cgcccggcgc
     4921 ggcttgtgcc cttcggtcgc gaggaacagc gcgaaaggaa gcgccaaatc ggcgaggggc
     4981 aacgcggtag cggtgccgac gctcgggccc ccacagctct tgttgtaaag gtacgacgcc
     5041 tcgagcagga ggacgccgaa gccgagcccg accgcggcga gctcggagat ggcagcgtcg
     5101 ggctcttcat tgcccgcctc gatgaggcga atgcaaccca gcatgcgcgc gatgctcgcg
     5161 gtgaaagcaa tcgggtgact cagcgccgaa cccggcatcg cgatcgagta gccgctctcg
     5221 gtcttcgaga gctggggcgc ctcggcgggt gctttctcgg ttccgcacgc tccgctgccg
     5281 cacccgcccg tgccgcaccc cgccgccttc ggcatgccgc tcagcgcgac ctcgatgtcg
     5341 accttttcca gcccggcgta cccttgcatg cgcgctatca ggcgctcgag cgactcctga
     5401 tcttgcttga actcgtcggg gaaccactcg ctcgtcggta gcaccaacgg ctgctgtccc
     5461 ggttcgagct cgagcgcagt cagcgcctcg ccataacgtc gcaccaccaa agaaagccgc
     5521 gcgggatcgg atagctccat gacgacgcga cgagtgtcgc agaaacgccg cactgcggcc
     5581 agtgacggtt ttgggacgaa gcattccgcg gttttgccgg cctccgcgcg cgcggcgttg
     5641 ggtctagaat cccggcatga aacgcatcgg cttcattggg cttggcacca tgggcagggg
     5701 aatggtgcgg aacttggttc gcaaggggta ccccaccacc gtttggaacc ggtcgcctgc
     5761 gactgtgtca cttctcggag tggaggtgag cgtcgcggcg accccgcgcg cgctcgccga
     5821 acaatgcgac gtcgtcattg cctgcgtgtc ggatccgccc gcggtggagc gcgtcgtgtt
     5881 cgccga
//
LOCUS       DASTKS010000997         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_827363, whole genome shotgun sequence.
ACCESSION   DASTKS010000997 DASTKS010000000
VERSION     DASTKS010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827363"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFQ35_21065"
     CDS             <1..1255
                     /locus_tag="VFQ35_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933214.1"
                     /translation="PAVALGFWAFTSCDETRHTTTEAPQTTEPSPNASIMPAPLASEI
                     EPEPKASVSHDAGAPTDAGSDAGVISTRFLREDSAVDPDLTPRENTGVRATMRLRWLD
                     LPPFPRLPEANLEALQRQREALAFELVVELSVGGRMRVRVNSDAFVWPRGTELRARLD
                     RVGNVLAWSAGTAYSVLPVGTLRAALNEGRPDSVPLSKPKLVTDGGGNVLGAATERIE
                     LSTPLGRLLLEQALLSNAGASGKLLCRALSELVAAEPLNAACERAYVPLRAELISRAG
                     GHLLIETIRLERDHVTLDPALLQTPPAAAAFRPLEIPSATPAQIPSSERLRELRLRAL
                     PRSEKPDPAAPKDGLVIQNRGDVLRYLLLDGLPVARVAARSELKLEGLLPGKYALATL
                     DFLGDDPASLRIVELPARVALGEEP"
     gene            complement(1319..1894)
                     /locus_tag="VFQ35_21070"
     CDS             complement(1319..1894)
                     /locus_tag="VFQ35_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933215.1"
                     /translation="MPTPPPPEIEELTLTCLDYVKRALGVALDFTPETLPLLDHYASI
                     ARAEIQANPALASIIAPAVGAYFGEVLRGRFDGFWRVPSQNPHDWTVCARTTFIAINP
                     IGVAYDAIYGGTDHDGPRSMLRVAPEDREYLDRRLETLPPVPEDEFYSFSTRFEVLEV
                     ATESLAAKMEEEGYGGTEYTEDDYGLDYSLN"
     gene            2281..3018
                     /locus_tag="VFQ35_21075"
     CDS             2281..3018
                     /locus_tag="VFQ35_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9933216.1"
                     /translation="MRLGILSDIHANYEALSAVIEAYKHENVDQYYCLGDTVGYGGSP
                     NECSDLIRDLVVATILGNHDAAVAGRMDYSYYYEAARHALDLHAQLLSAENMAWLKGL
                     PYQIKLDDLGVQLCHGSPVRLEEFEYIFAPEQARECLPIYEQLGQITLIGHSHLCKVF
                     ALTPTSVEELPPIDFELQPDRKYIVSVGSVGQPRDYDNRASFTVYDSVRRRFEFKRIE
                     YDIETAADKVLRAKLERNFAHRLFIGV"
     gene            complement(3063..>3367)
                     /locus_tag="VFQ35_21080"
     CDS             complement(3063..>3367)
                     /locus_tag="VFQ35_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933217.1"
                     /translation="AAFVAGAGELALRIEQAILRVGKLLGALRAPLSEYRARTKSRRL
                     SLRGLSEGAVQARVEARAMARLNKDFAESDRIRDELAALGVALRDSPEGTEWTIEQ"
BASE COUNT          583 a         1134 c         1101 g          549 t
ORIGIN      
        1 acctgcggtc gcgttgggat tttgggcctt cacgagctgc gacgagacac gtcacacgac
       61 gaccgaggct ccccaaacga ccgagccgag ccccaacgcg agcatcatgc cggcgccgct
      121 cgcgagcgag atcgagccgg agcccaaagc cagcgtgagc cacgacgcgg gcgcgcccac
      181 cgacgcgggc agcgacgcgg gcgtcatcag cacacgcttc ttgcgagaag acagcgcggt
      241 cgatccggat ctcaccccac gtgaaaacac gggcgtgcgg gctacgatgc ggctccgctg
      301 gctcgacctg ccgccgtttc cgcgcttgcc ggaggcgaat ttggaggctt tgcaacgcca
      361 gcgcgaagcg ctcgccttcg agctcgtggt cgagctctcg gtcggaggac ggatgcgtgt
      421 ccgcgtgaac tccgacgctt tcgtgtggcc gcgcggcacc gagctgcgcg cgcggctcga
      481 tcgcgtcggc aacgtgctcg cctggagcgc cggcaccgcg tattcggtgc ttcccgtcgg
      541 cacgctgcgc gcggcgttga acgagggacg tcccgacagc gttcccctat ccaaaccaaa
      601 actcgtgacc gacggcggcg gaaacgttct cggcgccgct accgagcgaa tcgagctctc
      661 cacgccgctc gggcggctcc tcctggagca agcgctgctc tcgaacgccg gcgcgtctgg
      721 aaagctcttg tgccgagcct tgagcgagct cgtcgcggcc gaacccttga acgcggcgtg
      781 cgagcgcgcg tacgtgccgt tacgggccga gctcatctcg cgcgccggcg gtcacttgct
      841 gatcgaaacg attcgcctcg agcgcgacca cgtcacactg gacccggcgc tacttcaaac
      901 cccccccgcc gccgcggcgt tccggccgct cgaaattccg agcgcaaccc ccgcacagat
      961 cccgagcagc gaacgcctac gcgagcttcg cttgcgcgcg ctgccgcgtt cggagaagcc
     1021 cgacccggca gcgccgaaag acgggctcgt cattcagaat cgcggcgacg tgctgcgcta
     1081 cctgctgcta gacgggctgc ccgtcgcgcg cgtggcggcg cgcagcgaac tcaagctcga
     1141 agggcttctg cctggcaagt acgcgctcgc aacgctcgac ttcttgggag acgatcccgc
     1201 ttcgctccgc atcgtcgaac tgcctgcgcg cgtcgcgctc ggcgaagagc cgtaggcttc
     1261 ctggttcgtg gcggcggtac gtgccgctct gcgattcggc gaacggctcg ggcctgcgtc
     1321 agttcaagct gtagtcgagg ccgtagtcgt cttcggtgta ttcggtgccg ccgtagccct
     1381 cttcttccat cttcgcggcg agcgattcgg tcgctacttc gagcacctcg aagcgggtgc
     1441 tgaacgaata gaattcgtct tcggggacgg gcggcaacgt ttcgaggcga cgatcgaggt
     1501 actcgcggtc ttccggggcc acgcgcagca tggagcgggg gccatcgtga tccgttccac
     1561 cgtagatggc gtcgtacgcg actccgatcg ggttgatggc gatgaacgtc gttcgtgcac
     1621 acaccgtcca gtcgtgcggg ttctgtgacg gcacacgcca gaatccgtcg aagcggccgc
     1681 gcaacacctc accgaagtag gcgcccaccg ccggggcgat gatggacgcg agcgcagggt
     1741 tcgcctgaat ctcggcgcgc gcgatgctcg catagtggtc gaggagcggc agcgtctccg
     1801 gagtgaagtc gagcgctacg ccaagggcgc gcttcacgta gtcgagacac gtcagggtga
     1861 gctcttcgat ttcgggcggg ggaggggtcg gcacggcggg agtctaacca gacgagggcg
     1921 gagcgcccgc tgcggagcaa agtgccggcc cggacagcgg tccgcggaag aaaagtcgac
     1981 ccgtcgggac gcacccgaac gctcattgac gagccgtcgg gcccggcggc cagcgtcccc
     2041 ggtcgggacg tatcccagac gccgtctgcc cccgagcgca cgaagggcgc caggcgtacc
     2101 ccaaaaatca gcccagaacg cgaggaacag ccgctcggcc gaggttttgg atgaccgcgt
     2161 accgaggaaa gtgacggggc gctcgaggaa ggacttgcac gagatgccca agctcgtgaa
     2221 ttgtgatcga aggctcgaca ggcggcgccc tgggcgcgtg ttatcctcgg ccgttacggc
     2281 atgcgcctcg gaatcctcag cgacatccac gccaactacg aggcgctgag cgccgtcatt
     2341 gaggcgtata aacacgagaa cgtcgaccag tactactgtc tgggcgacac cgtcgggtac
     2401 ggcggttctc cgaacgagtg ctcggatctc atccgagacc tagtggtagc gaccatcttg
     2461 ggcaaccacg atgccgccgt cgcgggtcgg atggactact cgtattacta cgaggccgct
     2521 cgacacgcac tcgacctgca cgcgcagctc ctgtcggcgg agaacatggc ctggctcaag
     2581 gggctcccct accaaatcaa gctcgacgat ttgggtgtcc agctttgcca tggctcgccg
     2641 gtccggctcg aggagttcga gtacatcttc gcgccggagc aagcccgaga gtgcctgccg
     2701 atttacgagc agctcggcca aatcacgctc atcggacact cgcacctctg caaggtgttc
     2761 gcactgacgc cgacctccgt cgaagagctg ccacccatcg atttcgagct gcagccagac
     2821 cgaaagtaca tcgtcagcgt gggctcggtg ggacagccgc gcgactacga caatcgcgcg
     2881 agtttcaccg tctacgactc ggttcgcagg cgcttcgagt tcaagcgcat cgagtacgac
     2941 atcgaaaccg ccgccgacaa ggtgctgcgc gcgaagctcg aacggaactt cgcgcatcgc
     3001 ttgttcatcg gcgtgtgagg ttggtcggct tcgagcgccg cacaacctca cctcgcgtta
     3061 cgtcactgtt cgatcgtcca ctcggtgcct tcggggctgt cgcgcagcgc gaccccaagg
     3121 gccgcgagct cgtcgcggat ccggtcgctc tcggcgaagt ccttgttgag acgcgccatc
     3181 gcgcgcgctt cgacccgcgc ctgaacggcg ccctcgctga ggccgcgcag ggatagccgc
     3241 cgagacttgg tgcgggcgcg gtactccgaa agcggcgctc gcagcgcgcc gagtagctta
     3301 cccacccgca aaatcgcctg ctcgatgcgc agcgcaagtt cgccggcgcc cgccacgaag
     3361 gccgcat
//
LOCUS       DASTKS010000998         7494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_380828, whole genome shotgun sequence.
ACCESSION   DASTKS010000998 DASTKS010000000
VERSION     DASTKS010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380828"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VFQ35_21085"
     CDS             complement(<1..1345)
                     /locus_tag="VFQ35_21085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933218.1"
                     /translation="MVEPAAAAIRLGPYLLGERLGLGGMAEVYVGQRAGPHGFAKKFA
                     IKRILPELAKDSRFVAMFVDEARICAALQHPNIVQVVDFGESQGELFMAMEFVDGVSV
                     AKLLRNIAGKRERFPDAIGLLIVRDVLRGLSYAHEATNEHGQPLGLVHRDVSPGNVLV
                     SRAGEVKLADFGIVRSAIVDRRTNPGELKGKLGYMSPEQVIGAEVDPRSDLFAVGILL
                     AEMLITCPLFAGKNELEVLTRIHEADLTTFEKHGAHVAPELQAIVRKALAREPRLRYQ
                     TARDFAGAIDAYADSKRIALDHAELSLWVTGLGVLASRSGTHAAQANVPAPPVNAAAP
                     TQGVDVDADREATTRAALPAPQREIRRPTQPALSLDDGRAAGRVDLLELVATGRVRRR
                     TKLVGRDGIGRPAVEVPGTAELLRRSAFQFGDPPVAEAAWSIPIQSRTLPARLYSL"
     gene            1446..2501
                     /locus_tag="VFQ35_21090"
     CDS             1446..2501
                     /locus_tag="VFQ35_21090"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9933219.1"
                     /translation="MSHETTPSHSVELSSSALGAPAPTRQSVAITGAASFLGTNLVGM
                     LEQDDSIERIVCLDVKPPATLGEKSRAYEVDLARPGAEERLAEVFAAERVDTLVHLAF
                     LPSPTHVAAWAHELESVGTMQVLNACRRTEVRKIVTWSQTLLYGAHPTNPNFLSERHA
                     LRAPRMEPFFVDKIHAEHEVLRYGQPGKGRVATVLRTAPILGPNVDTFITRYFSRRFV
                     PTVLGFDPLWQFLHEADAVAAFRLAVVRDAAGIFNITGHGVLPLHTVIRLIGRTAAPL
                     PRGLARTLAGAAWLTQLGEAPQSFCDYLQYICVADGALAERVLGFVPAYTSREAVSDF
                     ANAQRLRDVRLLTESPA"
     gene            2498..4189
                     /locus_tag="VFQ35_21095"
     CDS             2498..4189
                     /locus_tag="VFQ35_21095"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9933220.1"
                     /translation="MSSRRKNPHGRHDKPSNARPGARPNRGEAPRQGGRGREGTRDFG
                     RPSSNERGRDARYGAARPANGSRSGPDRARRDPHRDERREPPRAERREAPRAERREPG
                     RPVRRAAEKGGVLHTSLDRPPKAVPKAPRPAIEEPELPLIDEDFAGEDFAESEHADDF
                     AERAQGEDFAPSEDSDDSDESDEAEDFLDNGEHDADFMEEAPELGIGARVVPPNLRYE
                     GEATSSAPPPASPSPTRDVEAQIRALEARLDGLIRRAALTEEAETEAAEETQAISVAP
                     APFVATTTDTSTRDYVARRWGREALRDRSEEVDDFGLDPSFERKVQPGLELLYKRYFR
                     VQVEGMQHVPRAGRGVIVANHSGALPLDGVMLRTALRLDHPGVRDLRWLAEDFLFYLP
                     FAGVFMNRIGAVRACPENAERLLARDGLVAVFPEGVQGIRKLFRERYQLQRFGRGGYI
                     RLCLRMRAPLVPCAIIGGEETNPLVYRFDRLAEFLKLPYLPVTPTFPWLGPLGLLPAP
                     SRWKIRFGEPLSFDNYGPEAAEDDVLVGRLSERVRGSIQSLLDTGLRERKSVWFG"
     gene            4186..5070
                     /gene="truB"
                     /locus_tag="VFQ35_21100"
     CDS             4186..5070
                     /gene="truB"
                     /locus_tag="VFQ35_21100"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9933221.1"
                     /translation="MSEGPHGLLVVDKPAGVTSHDVVAAARRLFATRSVGHAGTLDPM
                     ATGVLVLLFGEACKLSGYLTADEKTYATTVRFGVATDSYDADGEVTEQRDLPPGFPEP
                     AALEAALVAERQRSEQVPPSVSAIHDRGVRAHVRVRRGETLALPPRPVAVHLLELRDR
                     QAESVSLELRVSKGYYVRSLARDLGEHLGVPAHLSALRRLASGAFTLADAHPWPSAVA
                     PALIELESAARRALPITELTEQGVTFARQGKRLAPEHARSTRTGAVSAWFGPDGRLVA
                     LGTSDEASHRVVRGFQQS"
     gene            5307..6203
                     /locus_tag="VFQ35_21105"
     CDS             5307..6203
                     /locus_tag="VFQ35_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933222.1"
                     /translation="MEIGRRWEIPTSYGWVLARLLEPTDPPLDEKPAALARREYARFQ
                     LLFELPRLSARGRAMLADLSRQLDPRRHSLALPWADTGVGDEEPRELERLITLESTTG
                     RLELEWRPYWTDALRERDPIEGELGPPRALPPPEPETTFINVNLVDQDGEPVIGQTCE
                     IELPDGTKRSTVTDFEGWARVRGFKQDGDAKITFPGVDELDFKGKPAGEKVIIPVQGE
                     AEEESEQVAPEETLTMGEGPNFLELAFVDVDGKPLADVPFEVSSDDGKKVSGKTDANG
                     LARVDGISPDKATVTLLESNSV"
     gene            6214..>7494
                     /locus_tag="VFQ35_21110"
     CDS             6214..>7494
                     /locus_tag="VFQ35_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9933223.1"
                     /translation="MVDVEFKQKESAGKSFSLPVNARHQVKLTPPTVEVLDVEDSHFN
                     FDRKVLLPELSEHDGSEPVLDTRRITGLGVIYAALSHAREHADQGLVVTGHTDASGTA
                     SYNQKLSEERAKNVSLLLRGEREQWRTQAAADDSVDDYQAVLLWQHQRAGWDCDPGAV
                     NNQLNVATKRAIGRFQELYNAEVDNAQSQGLDSPFKSKITVDKKVGKETWGAIYDVYM
                     SELMELLELENFSELEAIQKGLKAAPGMTDFVGCGEHIPFNPARRTPSKSNGEHDGPP
                     KNPPDRRVEILFFDPGEEVPLACHPAPGQCKPDVCPLYTLNPHRQQPIAVPKGLSIAE
                     LNLRLTYVDPEGNVRPFPEGLEVEAKFGDPKDDPGPPEPPPGDITLDPSDPKPAEPAP
                     TPTPDPNAPESMEVDQEPNEKIGADGLLRMVIPRK"
BASE COUNT         1287 a         2474 c         2514 g         1219 t
ORIGIN      
        1 cgagcgagta gaggcgagcg ggcagggtgc gggactgaat cgggatcgac caagcggctt
       61 cggcgacggg cggatcgccg aattgaaatg ccgagcggcg caagagctct gccgtgccgg
      121 ggacctcgac cgctggacgg ccgatgccat cgcggccgac gagcttggtt cggcgacgta
      181 cccgaccggt ggccacgagc tcgagcaagt cgacacgccc cgcggcgcgc ccgtcgtcga
      241 gggagagcgc gggctgcgtg ggacgccgta tttcgcgctg gggcgccggc aatgccgcgc
      301 gggtagtggc ttcgcgatct gcgtcgacgt cgacaccttg agtaggggcc gcggcgttca
      361 cgggcggagc cgggacgttg gcttgcgctg cgtgcgttcc gctgcgcgaa gccaggaccc
      421 cgagccctgt cacccagagc gagagctcgg cgtgatcgag cgcgatgcgc ttgctgtcgg
      481 cgtaagcgtc gatggcgccc gcgaagtcgc gcgcggtttg gtaacgaagg cggggttcgc
      541 gagcgagcgc cttacgaacg atggcttgga gctccggcgc aacgtgcgcg ccatgcttct
      601 cgaaggtcgt gaggtccgcc tcgtgaatcc gggtgagcac ctcgagctcg ttcttaccgg
      661 cgaagagtgg gcaagtaatg agcatttcgg cgagcaggat gccgaccgcg aacaagtcgc
      721 tgcgggggtc gacctcggcc ccgatgacct gctccggcga catgtagccg agcttgccct
      781 tgagctctcc agggttcgta cggcggtcga cgatcgcgct gcgcacgatg ccgaaatcag
      841 cgagcttgac ctcgccggct cggctcacga gcacgttgcc gggggagacg tcgcgatgga
      901 ccaagccgag cggttggccg tgctcgttcg tggcttcgtg ggcgtacgag agcccgcgca
      961 gaacgtcgcg cacgatgagc aggccgatgg cgtccggaaa acgctcgcgc ttgcccgcta
     1021 tgttgcggag cagtttggcc acggacacgc cgtccacgaa ttccatggcc atgaaaagct
     1081 cgccctggct ctctccgaag tcgacgacct gaacgatgtt cgggtgctgg agagcggcgc
     1141 aaatgcgcgc ttcgtcgacg aacatcgcca cgaaacggct gtctttcgcg agttcgggga
     1201 gaatcctctt gatcgcgaac ttcttcgcga acccgtgtgg ccccgcgcgc tggcccacgt
     1261 acacctcagc catacccccg aggcctaggc gctcgccgag caggtacgga ccgagtcgga
     1321 tggccgccgc ggcgggctcc accataagac cgtaagctta gacgacacgc tggctcgggg
     1381 ctacggtttc gactacggtc cgcccccatg ggcgacggcg cccccaacgt ctttctcgga
     1441 atagtgtgag ccacgaaact acaccttccc actcggtcga gctttcgagt tccgcgctcg
     1501 gcgccccagc gcccacgcgg cagagcgttg ccatcacggg cgctgcatcg tttctcggca
     1561 ccaatctcgt cggcatgctc gagcaagacg attcgattga gcgaatcgtt tgcctcgacg
     1621 taaaaccccc ggcaactctg ggagaaaagt cgcgggcgta cgaggtcgat ttggctcgac
     1681 ccggcgcaga agagcggctc gccgaagtct ttgctgcgga gcgcgtggac acgctcgtgc
     1741 acctcgcgtt cctcccgtcg ccgactcacg ttgccgcgtg ggcgcacgaa ctcgagagcg
     1801 tcggcaccat gcaggtgctg aacgcctgtc gtcgcaccga ggttaggaag atcgttacgt
     1861 ggagccaaac gctgctctac ggcgctcacc ccaccaaccc gaactttctc tccgagcgcc
     1921 atgccttgcg agctccgcgc atggagccct tcttcgtcga caagatccac gccgagcacg
     1981 aggtgctgcg ctacggccag cccggcaagg ggcgcgtcgc gacggtgctt cgaaccgcgc
     2041 cgatcctggg cccgaacgtc gataccttca tcacgcgcta cttcagtcgc aggttcgtgc
     2101 ccaccgtgct ggggttcgac ccgctttggc agtttttgca cgaggcggac gcggtggccg
     2161 cgtttcggct cgcggtcgtg cgcgacgcgg ccgggatctt caacatcact gggcacggcg
     2221 tgctgccgct ccacaccgtc attcgcctga tcgggcgcac ggcggcaccg ctgccgcggg
     2281 ggctagcgcg gaccttggct ggcgcggcgt ggctcacgca gctcggtgaa gcgccgcaga
     2341 gcttctgtga ttacctgcaa tacatctgtg tcgcggacgg cgcgctcgcc gaacgcgtgc
     2401 tcggtttcgt gccggcgtac acgagccgcg aggcagtgag cgatttcgcg aatgcgcagc
     2461 gtctgcgcga tgtccgcctt ctgacggaga gccccgcgtg agctcacgac gaaagaaccc
     2521 ccacggtcgt cacgacaagc ccagcaacgc gcgtcccggc gctcgtccca atcgcggtga
     2581 agctccgcgc caaggcggcc gagggcgaga aggcactcgc gatttcggga gaccgagctc
     2641 caacgaacgc ggccgcgacg cacgttacgg cgcggctcgt cccgcgaatg gcagtcgctc
     2701 ggggccggat cgcgcgcgcc gtgatcctca tcgcgacgaa cgtcgtgagc caccgcgcgc
     2761 ggagcgtcgc gaggcaccgc gcgcagagcg gcgtgagccg gggcgtccgg tgcgtcgcgc
     2821 tgcggagaag ggcggcgtgc tgcacacgtc tctcgatcgg ccgccgaagg ccgtgcccaa
     2881 ggccccgcgt cccgccatcg aagagcctga gttgccgctc atcgacgagg atttcgcggg
     2941 cgaggatttt gccgaaagcg agcacgcgga cgacttcgcc gaacgagctc agggggagga
     3001 ctttgcccct agtgaagact ccgacgactc ggacgagagc gacgaggcag aggactttct
     3061 cgacaacggc gagcacgacg ccgacttcat ggaagaagct ccggagctcg ggattggggc
     3121 acgcgtcgtg ccgccaaacc tgcgttacga gggtgaggcc acgtcgagcg cgccaccgcc
     3181 cgcgagccca tcgcctaccc gcgacgtcga agcacagatt cgcgctctcg aggcgcgtct
     3241 cgacggcctg attcggcgcg ccgcgctcac cgaagaggcc gagacagaag cggccgagga
     3301 aacacaggcg atcagcgtgg cgccggcgcc cttcgtcgcg acgaccaccg acacttcgac
     3361 gcgcgactac gtggcgcgtc gctggggccg cgaagccctg cgcgatcgct ccgaggaggt
     3421 cgacgacttc ggtctcgatc cgagcttcga gcggaaggtg cagccaggcc tcgagctcct
     3481 ctacaagcgc tatttccgcg ttcaggtaga ggggatgcaa cacgtgccgc gtgcaggccg
     3541 gggcgtcatc gtggccaatc actccggcgc gctcccgctc gatggcgtga tgctccgaac
     3601 cgcgctgcgc ctggatcacc ccggcgttcg tgacttgcgc tggctcgccg aggatttcct
     3661 cttttatctg ccttttgcgg gtgtcttcat gaaccgcatc ggcgcggtgc gtgcgtgccc
     3721 cgagaacgcc gagcggctcc tcgcgcgcga cgggttggtc gctgttttcc cggagggcgt
     3781 gcagggcatt cgcaagctct tccgtgaacg ctaccagctg cagcgcttcg gtcgtggcgg
     3841 ctacatccgc ctttgcttgc gcatgcgggc gcctctcgtt ccgtgcgcca tcatcggcgg
     3901 tgaagagacg aacccgctcg tctaccgctt cgatcgcctc gccgagttct tgaagctacc
     3961 gtatttgccg gtgacgccca cctttccgtg gcttgggccg ctcgggctgc tccctgcgcc
     4021 ttcgcgctgg aagatccgct tcggcgagcc gctctcgttc gacaactacg gtcccgaggc
     4081 ggcggaggac gacgtgctcg tcgggcgctt gtccgagcgg gtgcgaggca gcatccaaag
     4141 cctgctcgac accgggcttc gcgagcgtaa gagcgtctgg ttcggatgag cgagggtccg
     4201 cacgggctgc tcgtcgtcga caagcccgct ggcgtcacga gccacgacgt cgttgcggcg
     4261 gcgcggcgac tcttcgcgac gcgcagcgtt gggcacgctg ggacgctcga cccgatggca
     4321 accggcgtgc tcgtcttgct gtttggcgaa gcttgcaagc tttcgggcta cctgactgcg
     4381 gacgagaaga cgtacgcgac caccgttcgc ttcggggtgg cgaccgactc gtacgatgcc
     4441 gacggcgaag tcaccgagca acgcgacctc ccgcccggat ttcccgagcc cgccgcgctc
     4501 gaggctgctc tcgtggccga gcgtcaacgc tcggagcagg ttccgccgag cgtcagcgcc
     4561 attcacgatc gtggcgtgcg cgctcacgtg cgcgttcgcc gcggagagac tctcgcgctg
     4621 ccgcctcgac cggtcgcggt gcacctgctc gagctccgcg acaggcaggc tgaaagcgtg
     4681 agcctcgagc tgcgcgtctc caaaggatat tacgtgcgtt cgctggcgcg tgatctcggt
     4741 gaacacttgg gcgtaccggc gcacttgagc gcgctacggc gtttggcgag cggcgccttc
     4801 actctcgcgg acgcgcaccc ttggccgagc gccgtggcgc ccgcgctcat cgaactcgaa
     4861 tctgccgcgc gtcgcgcctt gcccatcacc gagctcaccg aacaaggggt caccttcgct
     4921 cgccagggca agcgcctcgc accggagcac gcacgctcga ctcgaacggg agccgtgagc
     4981 gcatggttcg gtcctgatgg acgcctcgtg gcgctgggaa ccagcgacga agcatcgcac
     5041 cgcgtggtgc ggggatttca gcagagctga cgtcgatcgc gctttgatcc gcgaaaacct
     5101 gaagcttacc gtcgagcagc gggttgctgc gattgctcgt caatagaggc gtcgatgggc
     5161 gcacttccgg cttgcatcga gttcgggggc cccgcgtaat cttgggaaat cacgccatcc
     5221 ccgccgcggt aacgtccgcg accacgaggg aattttcgga ggcttgtctg aatcggggaa
     5281 cggcgtatgc ttctccccgg gtttaggtgg aaatcggaag gcgctgggag atcccgacga
     5341 gctacggctg ggttttggcg cgcttgctcg agccaaccga cccaccgctc gacgagaaac
     5401 ccgccgcgct cgcacgtcgt gagtacgctc ggttccagct gcttttcgag cttccccggc
     5461 tgtctgcgag aggacgagcc atgctcgccg acttgtcccg tcagctggat ccccggcggc
     5521 attcgttggc gttgccctgg gccgacaccg gcgtggggga cgaggagccg cgcgagctcg
     5581 agcggctcat cacgctcgag tccaccaccg ggcgcctgga gctcgaatgg cgtccctact
     5641 ggacggacgc gctgcgcgaa cgcgatccca tcgagggtga gctcgggccg ccgcgcgcgc
     5701 tgccaccgcc cgaaccagag acgacgttca tcaacgtcaa cctggtcgat caggacggcg
     5761 agcccgtcat cggtcagacc tgtgaaatcg agctccccga cggtacgaaa cggagcaccg
     5821 taaccgattt cgagggctgg gcccgcgtgc gcggcttcaa gcaagacggc gacgccaaga
     5881 tcacttttcc gggcgtcgac gagctggatt tcaaggggaa accggcgggc gaaaaggtca
     5941 tcatcccggt tcagggagag gccgaagagg agtccgagca agtcgcgccc gaagagacgc
     6001 tcaccatggg ggagggtccg aatttcctcg agctcgcctt cgtcgacgtc gacggcaagc
     6061 cgctcgccga cgtcccgttc gaggtgtcgt cggacgacgg caagaaggtg agcggcaaaa
     6121 cggacgcgaa tgggctcgcg cgcgtggacg gaatctcgcc cgacaaggcg accgtcacac
     6181 tgctcgagag caactcggtt tgagggaggt cgagtggtag acgtcgagtt caaacagaag
     6241 gagtcggccg gtaagtcctt ctcgctgccg gtgaacgcgc ggcaccaggt caaattgacg
     6301 ccaccgacgg tggaggtgct cgacgtcgag gactcgcatt tcaacttcga ccgcaaggtg
     6361 ctgctgccgg agctctccga gcacgacggc agcgagccgg tgctcgacac gcgtcgcatc
     6421 accgggctcg gtgtgattta cgcggcgctg agccacgcgc gcgagcacgc cgaccaaggg
     6481 ctcgtcgtca cgggccacac cgacgcgagc ggcacggcga gctacaacca gaagctctcc
     6541 gaagagcgcg ccaagaacgt ctcgctcttg ctgcgtggcg agcgtgagca atggcggacg
     6601 caggcggccg cggacgattc ggtcgacgac taccaggccg tgcttctgtg gcagcaccag
     6661 cgcgcgggct gggattgcga tccgggggcg gtgaacaatc agctcaacgt cgcgaccaag
     6721 cgcgcgatcg gccgctttca ggaactttac aacgcagagg tcgacaacgc ccagtcacag
     6781 ggcctcgact cgcccttcaa gagcaagatc accgtcgaca agaaggtcgg caaggagacg
     6841 tggggcgcga tttacgacgt ctacatgtcg gagctgatgg agctactcga actcgagaac
     6901 ttctccgagc tcgaggcgat ccaaaagggc ctcaaggccg cgcccggcat gaccgacttc
     6961 gtcggttgcg gagagcacat tcccttcaac ccggcgcggc gcactccgag caagagcaac
     7021 ggagagcacg acgggccgcc gaagaacccg ccggatcgcc gagtcgaaat cttgtttttc
     7081 gatccggggg aggaagttcc gctcgcttgt catccggcgc cgggccagtg caagcccgat
     7141 gtctgcccgc tctacacgct gaatccacat cgtcagcagc cgatcgcagt cccgaaaggg
     7201 ctctcgattg ccgagttgaa tctgcgcctg acctacgtcg atccggaggg caacgtgcgc
     7261 cccttccccg aggggctcga ggtggaggcg aaatttggcg atcccaagga cgaccccggg
     7321 ccgcccgaac caccaccagg cgacatcacg ctcgacccgt ccgacccgaa gcccgccgag
     7381 cccgcgccga cgccgacgcc ggacccgaac gcacccgagt cgatggaggt cgaccaagag
     7441 ccgaatgaaa aaatcggcgc ggacgggctc ctccgcatgg tgattccgcg caag
//
LOCUS       DASTKS010000999         6082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1051169, whole genome shotgun sequence.
ACCESSION   DASTKS010000999 DASTKS010000000
VERSION     DASTKS010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051169"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2057
                     /locus_tag="VFQ35_21115"
     CDS             105..2057
                     /locus_tag="VFQ35_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9933224.1"
                     /translation="MRLADWFLGRPIATEEDDGERIGPFVGVGVLGLDALASASYGPE
                     ALLTVLLPLGTYGLSYLTPLVGATLVLLAFVALSYRQTIVAYPGGGGAYTVAKENLGE
                     RVSLLAAAALGLDYLLNAAVAVSAGVGALVSVLPNLLPYTLPLCLAVLAFLTLLNLRG
                     VRATGAAFMLPTYAFVATLGAVICIGIWKAVSHGGHPAPVVPPATVPAVVTSASHWVL
                     LRAFASGCTAMTGVEAVSNAVPIFKEPATTHARRSLSLIVGMLAFLLTGIAVLCRLYH
                     VTATPPGVAGYQSIVSQVVAATVGRNALYYVTLGSVVSVLALSANTSFADFPRVCRML
                     AGDRFLPEMFVHRGRRLAFSYGIVALGFFAALLLIVFGGITEGLIPLFAVGALAAFTL
                     SQAGMVAHWRKQSTPGARRSLVLNLIGALATGITLLVVLVSKFEQGAWITLVLVGAML
                     LLFRQVRRHYDFIAKATATEVPLEPTLVPALAVVPLRRWDALSVKALRFALGTGSEVV
                     AVQVLTGDREVDDLSERWDHIVEPLERAGVTRPRLVVLRSEYRQLIQPLVSFVLNSAS
                     EQPDRQVIVVVPELIEPHWYQRLLHNQTASVLKALLLLRGRPQIVIVTIPWYLRDWLP
                     ERRMLLRVRRALGFSRQRPRHPLQAP"
     gene            complement(2058..5984)
                     /locus_tag="VFQ35_21120"
     CDS             complement(2058..5984)
                     /locus_tag="VFQ35_21120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933225.1"
                     /translation="MTEGADTGNTDRDAAGAPLGGVPDGASSERGGALTGGGELGALI
                     RAMDWSKTPLGPISSWPQSLKTSVSLILNSQHPMWIGWGREATFLYNDAYVQVLSLAK
                     HPWALGRPFVEVWSEIWDVCGPLAAKVFERGEATFMEDVRLFMNRGDFIEETYYSFSY
                     SPIRDESGAVGGLFCPSAETTSRVLNARRLRTLSALSAGALAQKSTEAAYSSAEDTLA
                     ENPDDVPFALLYLVDESASTARRQCRVGLRNDPLLAPDAVPLGARATDAVWPIADVVG
                     SGQTRVVSLPAHPALPRDPAGQPVTRAVVLPLVSAGGNEAPSGVLIAAVNPARKLDAE
                     YLTFYDLIAGQVARAIQNARSVEEEKRRADALAELDRAKTMFFSNISHEFRTPLTLML
                     GPTEEALYSTDRALRGVGLETVHRNALRLLKLVNTLLDFSRLEAGRVQARFEAVDLAG
                     LTVDLASGFRSAMERGGLAFEVDCPPLPAPVPVDRDMWEKIVLNLLSNALKFTFEGSV
                     RVTLRSVDGRAVLEVSDTGVGIPEHELPKLFDRFHRIEGSRSRTHEGSGIGLALVRDL
                     VRLHHGELEASSRVGQGSTFRVSIPTDDAVLGERSPDTTEARPKNTAAYVQEALRWVP
                     APRAHGTREVTPAVLSEDVPHVLVADDNADMREYLERLLRPRFCVEVVADGAEALEAA
                     RRRRPDLVLADAMMPNLDGFALMRALRSDSELRTVPIIMVSARAGEEARVEGVEGGAD
                     DYLVKPFSARELLARVATQIELGRLRRMTELEGQRLQTIFAQAPIAIALVAGPEMRFV
                     LANDTYCTIAGSGDIIGKTLSEAFPELPPEHETFAAFRRALAGEALSSSERLVRLVRE
                     GRSEDSYWTTVWQPVRKADGRVSGVVVISTEVTDAVQARRRSEALGASLEQANRALEG
                     QNRDAIAAREEAEQANRMKDEFLATVSHELRTPLNAVLGWATLLRTGHRPDAERERAL
                     ATIERNARALARLVEDILDVSRIISGKLRLDVRRVELASVVQSAIDVVRPAAEAKQLS
                     LRVQLGEEPETILGDPDRIQQIIWNLLSNAVRFTPVGGHIDLSTEVHNGQLVLSVQDS
                     GMGIAAEHLPHVFERFRQVDSSTTRKHGGLGLGLAIVRHLVELHGGSVRAESPGIGQG
                     TRFTVSFPMRRSVSEAPSAARSDVSALVATPEARRVSLAGRSILVVDDEVDSRDIIAA
                     ALEGAGARVKLAASADEALEAVRANAPEILISDIGMPVHDGNYLIQRVRSLPADQGGA
                     LPAIALTAYARQEDADRSLSAGYQMHLAKPFDVASLVSAVARLLAD"
BASE COUNT          965 a         2059 c         1971 g         1087 t
ORIGIN      
        1 cgcgatgaaa agtcggggaa acactcgaca gcgccgcgct cccattcgaa cgccgattgt
       61 ttctggcaca tggcttgctc cggttcgccg ccttgagccc tcgaatgcgc ctcgctgact
      121 ggttcttggg gcggcccatc gcaacggaag aggacgacgg cgaacgcatt gggccgttcg
      181 tcggcgtcgg cgtcctgggg ctcgacgcgc tcgcgtcggc ttcgtacggc cccgaagcgc
      241 tgctgacggt gctgctgccg ctcgggacct acggcctctc ctacctgact ccgctcgtcg
      301 gagcgacgct cgttctgctc gccttcgtag cgctctcgta tcgccaaacg atcgtggcgt
      361 atcccggtgg cggcggcgct tacacggtgg ccaaggaaaa cctcggtgag cgcgtctcgc
      421 tcctcgccgc ggctgcgctc ggcctcgact acctcctcaa cgccgcggtc gccgtctcgg
      481 caggtgtcgg cgcgctcgtg tccgtgctgc cgaacctgtt gccgtacacg ctgccgttgt
      541 gcctcgccgt cctcgcgttc ttgacgctgc tgaacctgcg cggcgtccgc gccacgggag
      601 cggcgttcat gctgcccacg tacgctttcg tagcgacgct tggcgccgtg atttgcatcg
      661 ggatttggaa agcggtttcg catggcggac acccggctcc ggtcgtgccg ccagcgacgg
      721 tgccggcggt agtgacgagc gcatcgcact gggtcttgct tcgcgcgttt gccagcgggt
      781 gcaccgccat gaccggagtg gaagcagtga gcaacgccgt acctatcttc aaggaaccgg
      841 cgacaacgca cgcgcggcgc tcgctttcgc tgatcgtcgg gatgctcgcg ttcctgctca
      901 ccgggatcgc cgtcctgtgt cgcctgtacc atgtcactgc gacccctccg ggagtggcgg
      961 gataccaaag catcgtgtcg caggtcgtcg cggccaccgt tggtcgcaac gcgctctact
     1021 acgtgactct gggtagcgta gtcagcgtgc tcgcgctctc cgcgaacacg agcttcgcgg
     1081 actttcctcg cgtctgtcgc atgctcgccg gtgaccggtt cttgccggaa atgttcgtgc
     1141 accgggggcg ccgtctcgca ttctcgtacg gcatcgtggc cctcgggttc ttcgccgcgc
     1201 tcctgctgat tgtcttcggg gggatcacgg aaggcctgat tcccctattc gcggtcgggg
     1261 cgctcgcggc gttcacgctg tcgcaagcgg gaatggtcgc gcactggcgc aagcagtcga
     1321 cccccggcgc gaggcgctct ctcgtgctga acctcatcgg cgcgctcgcg acgggcatca
     1381 ccttgctcgt cgtgctggtt tcgaagttcg agcaaggcgc ctggatcacg ctcgtgttgg
     1441 tcggcgcgat gctgctgctc tttcggcaag tgcgtcggca ctacgacttc attgccaagg
     1501 ccaccgctac cgaagtcccc ctcgagccaa ccctcgtccc ggcgctagcc gtggtcccgc
     1561 tgcgtcgttg ggacgcgttg tcggtgaagg ccctgcgttt cgcgctcggg actggctccg
     1621 aagtggtcgc ggtccaggtg ctgacgggcg accgtgaagt ggatgacctc agcgagcgct
     1681 gggaccacat cgtcgagccg ctcgaacgcg cgggcgtcac ccgtcctcgg ctggtcgtgc
     1741 tccgctccga ataccggcaa ctgattcagc cgctcgtctc cttcgtgttg aactcggcga
     1801 gcgaacagcc agatcgccag gtcatcgtgg tcgttcccga gctcatcgag ccgcactggt
     1861 atcaaaggct cctccacaac cagaccgcct cggttctcaa ggctttgctc ctcttgagag
     1921 gccgccccca aatcgtgatt gtcacgatac cttggtattt gcgcgactgg ttacccgaac
     1981 ggaggatgct cttgcgagtt cgtcgagccc tcgggttttc gcgccagcgg ccgcggcatc
     2041 cgctgcaagc accgtaatca gtcggcgaga aggcgagcga ctgcgctcac gagcgacgcg
     2101 acgtcgaacg gcttcgccaa gtgcatttgg taccccgcgg agagagagcg atccgcgtcc
     2161 tcctggcggg cgtaggcggt gagcgcgatc gccggcagcg ctccgccctg gtccgccggc
     2221 agactacgga cgcgttggat caggtagttt ccatcgtgca cgggcatccc gatgtcgctg
     2281 atcagaatct cgggggcgtt ggctcgcacc gcctccagcg cttcatcggc cgatgcagcg
     2341 agcttcacgc gcgcgccggc gccctcgagc gcagctgcga tgatgtcgcg cgaatcgacc
     2401 tcgtcgtcga ccaccaagat ggaacgcccg gcgaggctga cgcggcgcgc ctcgggagtg
     2461 gcaaccaaag cagacacgtc gctccgagcc gcgctcggcg cctccgaaac gctgcggcgc
     2521 atggggaagc tcaccgtgaa gcgcgtccct tgcccaattc cggggctctc ggcgcgcacc
     2581 gagccgccgt ggagctcgac caggtgacgc acgatcgcga ggccgaggcc gaggccgccg
     2641 tgttttcgcg tcgtcgagct gtcgacttga cgaaaacgct cgaacacgtg cggcaggtgt
     2701 tccgccgcga tccccatgcc gctgtcctga acgctgagga ccaactgacc attgtgtact
     2761 tcggtactca agtcgatgtg cccgccgacg ggagtgaagc gcacggcgtt gctcaaaagg
     2821 ttccagataa tctgctggat gcgatccgga tctcccagaa tcgtctcggg ctcttcgccg
     2881 agctggaccc ggagcgatag ttgctttgct tcggcggcgg ggcgcaccac atcgatcgcg
     2941 gattggacca ccgacgcgag ctcgacgcgg cgcacgtcga ggcgcagctt tccgctgatg
     3001 atgcgcgaca cgtcgaggat gtcttcgacc agcctcgcga gcgcgcgtgc gttgcgttcg
     3061 atcgtcgcga gcgcgcgttc tcgctccgca tccggccgat gcccggttcg cagcagcgtc
     3121 gcccaaccga gcacggcgtt cagcggcgtc cgaagctcgt ggctcacggt ggcgagaaac
     3181 tcgtctttca tgcggttcgc ctgctccgcc tcttcacgcg cggcgatggc gtcgcggttt
     3241 tgtccttcga gcgcgcgatt cgcctgctcg aggcttgccc cgagcgcctc gctgcgacgg
     3301 cgcgcctgca ccgcgtcggt cacttcggtg gagatcacga cgacgccgga cacgcgtccg
     3361 tccgcctttc gcaccggctg ccagaccgtc gtccagtagc tgtcttcgct acggccctcg
     3421 cgaaccaagc gcaccaatcg ctcactcgat gacagcgcct cgccagccaa ggcgcgtcga
     3481 aacgccgcga acgtctcgtg ctccggaggc aactccggga acgcctcgct cagcgtcttg
     3541 ccgatgatgt cgccgctgcc cgcgatcgtg caatacgtgt cgttcgcgag aacgaagcgc
     3601 atctcgggac cagcgacgag cgcgatcgcg atcggtgcct gcgcgaagat ggtctgcaat
     3661 cgctgcccct cgagctccgt catccgacgt agacggccga gctcgatctg cgtcgcgacg
     3721 cgcgccagga gctcgcgcgc ggagaacggc tttaccaagt aatcgtcggc gccgccctcg
     3781 accccctcga cgcgcgcttc ttcgccggca cgcgccgaaa ccatgatgat gggtacggtg
     3841 cgtagctcgg agtcagatcg cagcgcgcgc atcagcgcga acccgtccag gttcggcatc
     3901 atcgcgtcgg cgagcacgag gtcggggcgc cgccggcgcg ccgcttcgag cgcctcggca
     3961 ccgtccgcga cgacctcgac gcaaaagcgc ggtcgcagga ggcgctccag gtactctcgc
     4021 atgtcggcgt tgtcgtcggc cacgagcacg tgggggacgt cttccgaaag caccgccggc
     4081 gtgacctcgc gagtcccgtg agcgcgcggc gccggaaccc agcgcagcgc ctcctgcacg
     4141 taggcggcgg tgtttttcgg gcgcgcctcc gtcgtatcgg gcgatcgctc accgagcacg
     4201 gcgtcatcgg tcgggatcga gacccggaat gtgctcccct gaccgacgcg actcgatgct
     4261 tcgagctcgc cgtgatgcag cctgaccagg tctcgcacca acgcgagccc gatgccggac
     4321 ccctcgtgag tacgcgaccg gctgccttcg atgcgatgga agcggtcgaa gagcttcggc
     4381 agctcgtgct ccggaatgcc aacgccagta tcggagacct cgagcaccgc gcggccgtcg
     4441 acggagcgaa gcgtaacgcg aacgctgcct tcgaacgtga atttgagcgc gttcgacagc
     4501 aggttcaaga cgatcttttc ccacatgtct cggtcgaccg gaacgggagc gggaagcggc
     4561 ggacagtcca cctcgaatgc gagtccgccg cgctccatcg cggagcgaaa accactcgcc
     4621 aaatcgacgg tcaacccagc tagatccacc gcctcgaagc gcgcttgcac gcgcccggcc
     4681 tcgagtcgtg aaaagtcgag gagggtgttg accaacttga gcaaccgaag cgcgttgcgg
     4741 tggaccgtct cgaggccaac tccgcgcaat gcgcgatccg tggagtagag cgcttcctcg
     4801 gtcggaccga gcatcagcgt gagcggagtg cgaaactcgt gactgatgtt cgagaagaac
     4861 atcgtcttcg cgcggtcgag ctccgcgaga gcatccgcgc gacgcttttc ctcctcgacg
     4921 ctgcgagcgt tttggatcgc gcgggcgacc tgtcccgcga ttaggtcgta gaacgttaga
     4981 tattcggcgt cgagcttgcg cgctgggttg acggcggcga tgagcacacc ggatggggct
     5041 tcgttgccgc cggccgaaac caagggcaag accactgccc gcgttacggg ctggcctgct
     5101 gggtcgcgcg gcagcgctgg gtgcgcaggc agcgatacga cccgggtttg accgctaccg
     5161 acgacgtctg cgatgggcca caccgcgtca gtcgccctcg caccgagcgg aaccgcgtcc
     5221 ggcgcgagca gcgggtcgtt tcgaagcccc acgcggcact ggcggcgggc ggtgctggcg
     5281 ctctcgtcga cgaggtagag caacgcgaag gggacgtcgt cggggttctc cgcgagcgta
     5341 tcttccgcag acgaataggc cgcctcggtc gacttctgcg cgagcgcacc ggcgctcaac
     5401 gccgacaacg tgcgcaggcg gcgtgcgttc aatacgcgcg acgtcgtctc ggcggagggg
     5461 caaaacagac cgcccacggc acccgactca tcgcgtatgg ggctgtaaga aaatgagtag
     5521 taagtctcct cgatgaagtc accgcggttc atgaagagcc gcacgtcttc catgaacgtt
     5581 gcttcaccgc gctcgaagac tttcgcggcg agaggcccgc acacgtccca aatctccgac
     5641 catacctcta cgaaaggacg cccgagcgcc cacgggtgct tcgcgagact cagcacctga
     5701 acgtaagcgt cgttgtacaa aaacgtcgct tcgcgacccc agccgatcca catcgggtgc
     5761 tgcgaattga ggatcaggct gaccgatgtc ttgaggctct gaggccaaga actaatcggg
     5821 ccgagcggcg tcttcgacca gtccatggcg cgaatcaggg cgccgagctc cccgccgccg
     5881 gtgagcgctc cgccgcgctc gctcgaggca ccgtcaggga ctccacccag cggtgcgccg
     5941 gcagcgtcac gatcggtatt cccagtatcg gctccttcgg tcatcgactc gatcgccgcg
     6001 tgccccgccg cggccgtcca cgtgatgttc aggtcgtgcg gtgcgggctg acgagtctaa
     6061 acgctcccgc catggcggga ga
//
LOCUS       DASTKS010001000         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1499868, whole genome shotgun sequence.
ACCESSION   DASTKS010001000 DASTKS010000000
VERSION     DASTKS010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499868"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="ybeY"
                     /locus_tag="VFQ35_21125"
     CDS             complement(<1..479)
                     /gene="ybeY"
                     /locus_tag="VFQ35_21125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9933226.1"
                     /translation="MRRLAEAMLRTLELPHSELSVLLTDDTTIHELNRQHRQKDKPTD
                     VLAFPLDEKGEDPARPWLGDVVISIDTALRQAESRRRELVAEVRFLLAHGLLHLIGYD
                     HDTKPRKREMDAAARRLVRSAPLEERLTVRPDAANPHLAAKPRADGKPPATAKARAKV
                     "
     gene            738..1307
                     /locus_tag="VFQ35_21130"
     CDS             738..1307
                     /locus_tag="VFQ35_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933227.1"
                     /translation="MARPSRPPGFDMTYRPAETAFGPPMRVKVPSLIYLGVALAAVMV
                     VVIAEKSPAGSFLYNHIVARGAHGFVGARTVALMLVLGAISSLVRASMRGVRIRADGV
                     EYRDVVSFGLPKLRRYRWAQIDRVVLDLPDCVAFDVWDGSRAFLPEVSDRAGLSAALE
                     KIAAARAIPVRGGAGLDEIPESPELAEES"
     gene            complement(1400..2941)
                     /locus_tag="VFQ35_21135"
     CDS             complement(1400..2941)
                     /locus_tag="VFQ35_21135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933228.1"
                     /translation="MASSGAEEDGPEAGAAPNVEVSSVGSRAVRPADVDDTPRALAGK
                     TIGRYRLLYPVAAGGMAQVWTAKPEGGGLARTVAIKLVRPEYAADEEYSRMFIDEAMV
                     ASSIHHPNVCEIYELGRDGDLLFMVLEWVAGDSLSGLVQRGAELSPLPDHVAARIVAD
                     ACAGLHAAHEAIGQDGQPLGIVHRDVSPPNILVSMHGHVKVSDFGIAKARHQLHSRTR
                     TGEIKGKFAYIPPEQILGRGVDRRADIYAMGCVLYVASLGLRPFGGGASALGKIVQGK
                     FRLPSELRADYPEELQAIVVKSLAADPNQRYQTAEEMRLALEQWMLTSSKLATPSDVA
                     RIVRERMNPERRRLVEAVMSQGRSLPEALAYQLLSISDKTDTPTATSGLVVQPDGLGK
                     KGKAKSKARPEQDSVSDTDATLVASITTEQSRSGTLKGLGGQQPSATRSERPAVVTAR
                     QRQDDAVTSRPPSRRDSGVPQPRISKHPSSAPPEFRGGVSQWVFWMLGLAVVLLVLAF
                     ALGKH"
     gene            3371..>3846
                     /locus_tag="VFQ35_21140"
     CDS             3371..>3846
                     /locus_tag="VFQ35_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9933229.1"
                     /translation="MSELLTQRPPAPSSRFELVVYMRQQSRMYPLTPGSVVDIGRSSD
                     NQVQIDDPSVSRHHARLHVEGEVQVEDLGSANGTSLVRASKSRADLDETDASANQRLE
                     PNQLATLVPGDVLRVGAVILVLQRKGRSSLPTPSGEVVQAGPSVLVDPEMRRVYDLA"
BASE COUNT          589 a         1204 c         1330 g          723 t
ORIGIN      
        1 actttggcgc gggctttggc ggttgcggga ggtttgccgt ctgcgcgagg tttggccgct
       61 aggtgtgggt tagccgcatc gggtcgaacg gtgagtcgtt cttcgagcgg cgcgctgcgc
      121 acgaggcgcc gggctgctgc gtccatctcg cgtttgcgcg gcttcgtgtc gtgatcgtag
      181 ccgatgagat gcaagagtcc gtgcgccagc aggaagcgca cttccgcgac gagctcgcgt
      241 cggcgtgatt ccgcttggcg aagtgccgtg tcgatcgaga tgacgacgtc gcccagccac
      301 ggccgtgctg ggtcttcccc cttctcgtcg agcggaaatg cgaggacgtc ggtcggctta
      361 tccttctgcc ggtgctgacg attcagctcg tgaatcgtcg tgtcgtcggt gaggagcacg
      421 ctgagctcgg agtgggggag ctcgagcgta cgcagcatcg cctcggcaag gcggcgcacg
      481 actacggggg acagtgccgc ggagcgcggc gcacggcgag tgacgagggc aggcaagcgg
      541 aagcggcctt aagcggaagc gaggctcggc gccaggaaaa tggctcaacg gacgctgtgt
      601 agtccgcttt ggctagataa ttgtattgcg actcgcccgt tcgccgcggg gtagccggcg
      661 gtcgcggacg ggcggccgcg gacctgacca ggtgctcggc tttccaaggt tgaggtcgag
      721 agttacgttt caatcacatg gctcgtccgt cgcgtccgcc tggcttcgac atgacctatc
      781 ggcccgcgga gaccgcgttt ggcccaccga tgcgggtgaa ggtcccgtcg ctcatctatc
      841 tgggggttgc cctcgctgcg gtcatggtcg tcgtcatcgc ggagaagagc ccggccggca
      901 gctttctcta caaccacatc gtggcgcgcg gcgctcacgg cttcgtcggc gcgcgcaccg
      961 tcgctctcat gctcgttttg ggcgcgattt cgtccttggt gagggccagc atgcgcggtg
     1021 ttcgcattcg cgcggacggc gtcgagtatc gcgacgtcgt ctccttcggt ctgccgaagc
     1081 ttcggcgtta ccgctgggcg cagatcgatc gcgtggtctt ggacttgcca gactgcgtgg
     1141 cgttcgatgt ctgggatggt tctcgcgcct ttttgcccga ggtgtcggac cgagcgggcc
     1201 tttcggcggc tctcgagaag atcgcggccg cgcgcgcgat tccggttcgc ggcggcgcgg
     1261 gcctcgacga aatccctgaa tcgcccgagc tcgccgaaga gtcgtaagcg ctctttcggg
     1321 cgatcgttgc gcggggcgag cggcgatgcg cggggcgagc ggcgatgcgc gccgatgcgc
     1381 tagtgagtac gtcgctcgct caatgcttgc cgagcgcgaa cgcgagcacc agcaaaacca
     1441 ccgccaagcc cagcatccaa aatacccatt ggctcacgcc gccgcggaat tctggaggcg
     1501 ccgaagacgg gtgcttgctg atccggggct ggggcacgcc ggagtcgcgc cgggagggcg
     1561 ggcgagacgt gacggcatcg tcctgtcgct gtcgcgctgt gacgaccgcg gggcgctcgg
     1621 agcgggtggc ggaaggctgt tgcccaccga ggcccttgag cgtcccggag cgactctgtt
     1681 cggtcgtgat cgaggctacc aaggttgcgt ccgtgtccga gacggagtcc tgctccggac
     1741 gagccttgga tttcgctttg cccttcttac cgaggccgtc gggctggacg acgaggccgc
     1801 tcgttgccgt cggcgtgtcg gtcttgtccg aaatcgaaag cagctggtac gcgagcgcct
     1861 cgggcaaaga gcggccctgg ctcatcaccg cctcgaccag gcggcgcctc tcgggattca
     1921 tgcgttcgcg cacgattcga gccacgtcgc tcggcgtcgc gagcttggac gacgtaagca
     1981 tccactgctc gagcgcgagc cgcatctctt cagctgtttg gtagcgttgg ttcggatccg
     2041 cagcgagtga tttgacgacg attgcctgga gctcctccgg gtagtcggcg cggagctcgc
     2101 tcggcaggcg gaacttccct tgaacgattt tgcccagcgc cgacgcgccg ccgccgaaag
     2161 gccgcagccc gaggctcgca acgtacagca cgcaacccat cgcgtagatg tccgcgcgcc
     2221 ggtcgactcc tcgaccaaga atctgctcgg gaggaatgta agcgaacttg cctttgatct
     2281 ctccggtgcg tgtacgtgag tggagctggt gccgcgcctt cgcgatgccg aagtcgctca
     2341 ccttcacgtg cccgtgcatc gacaccaaaa tgttcggcgg cgagacgtcg cggtgcacga
     2401 taccgagggg ttgtccgtct tgacctatcg cctcgtgcgc cgcatgcagg cccgcgcacg
     2461 cgtcggccac gatgcgcgcg gcgacgtgat ctggcagggg cgagagctct gcgccgcgct
     2521 ggacgaggcc gctcagcgaa tctcccgcga cccactcgag gaccatgaac aggaggtccc
     2581 cgtcgcgccc gagctcgtaa atttcgcata cgttcgggtg gtgaatcgac gaggccacca
     2641 tcgcctcgtc gatgaacatg cgcgagtact cctcgtccgc ggcgtactcg ggccgcacca
     2701 gcttgatggc aaccgtgcgc gcgagcccgc cgccctctgg tttggccgtc caaacctgcg
     2761 ccatgccgcc cgcagcgaca gggtagagca ggcggtaccg tccgatggtc ttgcctgcca
     2821 gggcgcgcgg cgtgtcgtcg acatccgccg gccgtactgc gcgtgacccc accgacgata
     2881 cctcgacatt gggcgcggcg cccgcttcgg ggccgtcttc ttcagcgccg ctgcttgcca
     2941 tacgaattcg aatctaaccg agttagctgc gccaatctag cgcttttgcc gcgcgctccg
     3001 gcgcgcggga gcgacgcgcg gttggggcga gaaaatgctc cacgacgcac cccttcgggc
     3061 gtcgtccgac aacgtgggaa acgtgggaga ggatttcggg cggggcgctt ttgagcattg
     3121 cgtgccgtac gcggcacgcc tttgaaacgc gggcgagggg cggcgcattc gagcggtcgg
     3181 ttacgtaagt tctcttccgt cgatcgagag gtttggcggt cattcgcggc tttttggaga
     3241 acgcgagcag ggcgacggtg gtgtatccta ggggtccgag tggcagaacg acgtgggcaa
     3301 agcaggttct tcgcgctatc ctttcgaaag ttgcgatcgt tcgcggatcc gaacgggtga
     3361 ctgaaggcaa atgtcggagc ttctcaccca acgtcctccc gccccgtcga gccgattcga
     3421 gctcgtggtt tatatgcgcc agcagtcgcg catgtatccg ctgacgccag gcagcgtggt
     3481 cgacatcggg cgaagttcgg acaaccaagt gcagatcgac gacccgtccg tgtcgcggca
     3541 ccacgctcgg ttgcatgtcg agggcgaagt tcaagtcgag gatctcggca gcgcgaacgg
     3601 cacgagcctc gtccgggcct cgaagtctcg cgcagatctg gacgagaccg acgcgagcgc
     3661 gaaccagcgg ctcgagccga accagctcgc gacgctcgtt cccggcgacg tcctgcgcgt
     3721 cggcgcggtg attttggtgc tgcagcgcaa gggccgctcg agcctgccca ctccatctgg
     3781 cgaggtggtt caagccggtc cgtcggtgct cgtggaccca gaaatgcgtc gcgtgtacga
     3841 cctcgc
//
LOCUS       DASTKS010001001         5365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1499942, whole genome shotgun sequence.
ACCESSION   DASTKS010001001 DASTKS010000000
VERSION     DASTKS010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499942"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFQ35_21145"
     CDS             complement(<1..309)
                     /locus_tag="VFQ35_21145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933230.1"
                     /translation="MRVIQWLGIVASSSLVAIGGLLVALAPSARAQSTPTLPPGLPLP
                     QQQEQQRFDILASLDFLRDRPPGLCYLPAPTALSDIDVHRSLFVHDRATLDANGGGLF
                     T"
     gene            complement(458..1396)
                     /locus_tag="VFQ35_21150"
     CDS             complement(458..1396)
                     /locus_tag="VFQ35_21150"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9933231.1"
                     /translation="MKAFICTAYGPPNVLEPREVPAPAPRRDEVLIRVRAAGVTASDI
                     FIRSSQVPLRLLIPMRLMLGITKPRKGIIGEVLAGEIAAVGEGIRRFEVGDRVAAVTG
                     FSLGAYAEYKCMKEVDSKRGCLTHIAKNIGFEAATVAAYGGALALQFLERDRIQPGRR
                     VLVYGASGTCGTIAVQLAKYWGAEVTGVCSAAHVELVRELGADEVLDYSLDDSLEPNA
                     CFDFVLDAVGRAKTSKLKDACRRALAAGGKYASIDDAALQLDSGRLERLNELIETGHV
                     RPVVDRTYPFEKLAEAHDYVERGHKAGGVAVSIGDR"
     gene            complement(1430..2167)
                     /locus_tag="VFQ35_21155"
     CDS             complement(1430..2167)
                     /locus_tag="VFQ35_21155"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9933232.1"
                     /translation="MTSDSRNGTQRVKKVCVVGASGKLGRYLVQHALARGYEVVGVCR
                     AQSVDKLADFAGRITIVPGMTDDSDVIRRAVAGCDGVLVVLFPWGMKGYSSGTAKAVL
                     QHARPGARLVFSCGWHISFDGKDVYPRSMKLLEKATTVIGHLLRVVDVPDQVRACQSV
                     FSSDTRWTVVRGSDLEEGESEGLPVWSRHVGDPILASNRTRRTDFALFMVEALAHDEL
                     VHEAPAIVSCKAPSARGAMDPATAESA"
     gene            complement(2381..3451)
                     /locus_tag="VFQ35_21160"
     CDS             complement(2381..3451)
                     /locus_tag="VFQ35_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9933233.1"
                     /translation="MRASKNWVDDSFRNVHPVLPALRTGEKMPSIGEFLCGGGRRSPV
                     APLPTLDPRAPWQKAPETGLRATWLGHSTVLLEIDGQRVLTDPVWGERASPVPLVGPK
                     RFQAVPVELKELPPLDAVLISHDHYDHLDYPTIRALVASDVPIVTSLGVGAHLEAWGI
                     APARITELDWWESTRVGGLTVTAAPSQHFSGRGMGDRNATLWSSLVVQGSRHACFFSG
                     DTGLTTEYESIAARLGPFDLVMLEVGAFHPAWGDIHLGPEHALDACKLLSSKRFLPIH
                     WGTFNLALHAWDEPAETLLARAPAHGVDLVMPRIGEAIEPARYESPTPWWRAIAALEK
                     RAAPQLQAPAETLDEGVGFPID"
     gene            complement(3573..4205)
                     /locus_tag="VFQ35_21165"
     CDS             complement(3573..4205)
                     /locus_tag="VFQ35_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933234.1"
                     /translation="MSASDSTRASTDVDLAALPPPRRPFRRLTFVVMGVTALFALRLA
                     LGLRGELVYTLRGGAPTDLGELGPRAPTALTPNSWVRAEGALSDRDVVRYSRPLESDS
                     YRLSALADNPKVWVELRVPRDTDETHFVAPGSFVGRLVPARGAGLRYRALETAAEASG
                     HGLPRDAWLLIDGESPSGTRWVLALEAMLIAFAAFNVAGIFRLAKPVRDA"
     gene            4506..5042
                     /locus_tag="VFQ35_21170"
     CDS             4506..5042
                     /locus_tag="VFQ35_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933235.1"
                     /translation="MATANKITCPSCGFQNTPPLANNRCVSCGAKIEDIKRQLTRQEE
                     IERRYQQEGFSMQWFGVSLAIIIVMTAALVMGLPMVVPALDFEGSAGMLVAIPVWFLG
                     GLLIGLISPGRTFIEPVVAVFLVAIPTAFLLFQGQTVKTMPAFMYALMSVLGVLFTLI
                     GSYIGERIQLGPPPKPSE"
     gene            5052..>5365
                     /locus_tag="VFQ35_21175"
     CDS             5052..>5365
                     /locus_tag="VFQ35_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933236.1"
                     /translation="MRTSFAELAALLLSALLVSNCRSSEAREREAEFARIGHSIDALR
                     NAPNEGKAPLIQALEKQECRERSACDLKETCISAYRLLDSARGASANAKTLLANRDGG
                     AK"
BASE COUNT          932 a         1739 c         1766 g          928 t
ORIGIN      
        1 cgtgaagagc ccaccaccgt ttgcgtcgag tgtcgcgcgg tcgtgtacga agagcgaccg
       61 atgcacgtcg atgtcggaga gcgcagtcgg cgcgggcaag tagcagagcc ccggcgggcg
      121 gtcgcgtaag aagtcgagtg acgccaggat gtcgaagcgc tgttgctcct gctgttgagg
      181 aagcggcaag cccggcggca aggtcggagt ggattgtgcg cgcgccgaag gcgccagcgc
      241 gacgagcaag ccgccgatgg cgaccaagct cgagcttgcg acgataccca accactgaat
      301 cacgcgcatg acgaactcct cgcgtagacc ccaaagcaga gaccgcgaac tcggcgtccg
      361 agatcttctc gcctttgggg gttacgtgct gcgcaggttc aggtcaatag cgattgaatc
      421 acgtctttgg gtcgcgagcg tttgctgcca gccaaattca ccgatctcct atgctgaccg
      481 caaccccgcc cgccttgtga cctcgctcga cgtagtcgtg cgcctcggcg agcttctcga
      541 aggggtaggt gcggtccacc accgggcgaa cgtgcccggt ttcgatgagt tcgttgagcc
      601 gctcgaggcg ccctgaatcg agctgcaagg ccgcatcgtc tatggatgcg tacttgccac
      661 ctgcagccag cgctcgccta cacgcatcct tgagcttcga cgttttggcg cggcccacgg
      721 cgtccagtac gaagtcgaag catgcgttcg gctccaaaga gtcatcgagg gaatagtcga
      781 gcacctcgtc tgcgccgagc tcgcgcacga gctcgacgtg cgctgcgctg cacacgcccg
      841 tgacctcggc cccccagtac ttcgccagct gcacggcgat cgtcccgcag gtgccggagg
      901 cgccgtacac gagcacgcgg cgcccaggtt ggattcggtc gcgctccagg aactgaagcg
      961 cgagggcgcc gccgtaggcc gcgaccgtcg cggcttcgaa gccgatgttc ttggcgatgt
     1021 gagtcagaca gccccgcttc gaatcgacct ctttcatgca tttgtactcg gcgtaggcgc
     1081 ccagactgaa tccggtgacg gcagcgaccc tgtctccgac ttcgaagcgg cggatgcctt
     1141 caccgacggc cgcgatctcg cctgcgagca cctccccgat gatgcctttg cggggcttcg
     1201 tgatcccgag catcaaacgc atcgggatga gcaagcgaag tggcacctgc gaggagcgga
     1261 tgaagatgtc gctcgcagtt acgccggcgg cgcgaacccg gatcagcacc tcgtcgcgcc
     1321 gcggcgcggg cgctggaacc tctctcggtt cgagcacgtt gggcgggccg tacgcggtac
     1381 aaatgaatgc cttcacgatt gccgctcctt tgcgggcgct cgggcgcgct cacgccgact
     1441 ccgcggtagc aggatccatc gcgccccgtg ctgacggagc cttgcagctg acgatggccg
     1501 gcgcctcgtg cacgagctcg tcgtgggcca gcgcttcgac catgaagagc gcgaagtcgg
     1561 tacgccgagt ccgattgctc gcgagaatcg ggtcgccgac gtggcgactc cagaccggca
     1621 agccctcgct ctcgccttct tcgagatcgc tgccgcgcac gaccgtccag cgcgtgtcgc
     1681 tcgaaaacac cgattggcat gcccggactt gatccggaac gtcgaccacg cgcagcaagt
     1741 gcccgatcac ggtggtcgct ttttcgagca gcttcatgct tcgcgggtac acgtccttgc
     1801 cgtcgaacga gatgtgccag ccgcacgaga acacgagcct ggctccgggc ctcgcgtgct
     1861 ggagcaccgc cttcgcggtc ccggaggaat agcccttcat cccccaggga aacagcacga
     1921 cgagcacgcc gtcacagcct gcaaccgcgc ggcgaatgac gtcgctgtcg tcggtcatcc
     1981 ccggcacaat cgtgattcgt cccgcgaaat cagcgagctt gtcgacgctc tgagctcggc
     2041 acacgccgac tacttcgtat cccctcgcga gcgcgtgctg aaccaggtac cgaccgagct
     2101 ttccggaagc gcccaccaca cacacctttt tcacgcgctg cgtgccgttg cggctgtcgc
     2161 tggtcatgac gcgctcccgg gtcactacgg ctccgagatc gttccggagg cagctaaaag
     2221 cgatgcatcg cgcgtgccgc tactcgtcac tctcgagttt ctgcgggtgt tcgcacggcg
     2281 ttcggctgcc tcccgcaaag actgcgctgc gggccgactc tcgtgcgcaa gcgcttcgct
     2341 gcgcggcgcg agtcgagtgg tcggattgcg gagcagcgga tcagtcgatg ggaaagccga
     2401 cgccctcgtc gagagtttcg gcgggggcct ggagctgcgg cgcggcgcgc ttctcgagcg
     2461 cggcgatggc ccgccaccag ggcgtcggcg actcgtagcg ggcgggctcg atcgcttcac
     2521 cgatgcgcgg catcacgagg tcgacgccgt gcgctggggc tcgcgccagc agagtctcgg
     2581 ccggctcgtc ccatgcgtgg agcgcaaggt tgaacgtgcc ccaatggatc ggcaggaatc
     2641 gtttcgatga gagcagcttg caagcgtcga gcgcgtgctc gggtccgaga tggatgtctc
     2701 cccacgcggg atgaaaggcg ccgacctcga gcatgacgag atcgaagggg ccgagacgcg
     2761 cggcgatgga ttcgtattcg gtggtgagcc ccgtgtctcc gctgaagaag caggcgtgcc
     2821 gcgagccttg caccacgagc gacgaccaca gcgtcgcgtt gcggtcgccc atcccacggc
     2881 cggagaagtg ttgagacggc gccgcggtga cggtgagccc gccaacgcga gtcgactccc
     2941 accaatcgag ttcggtgatg cgcgcgggag cgatccccca cgcctcgagg tgcgcgccaa
     3001 ctcccagcga ggtcacgatg ggaacgtcgc tcgcgacgag cgcgcgaatc gtcgggtagt
     3061 cgaggtggtc gtagtggtcg tgtgagatga gcaccgcgtc gaggggagga agctctttga
     3121 gctcgaccgg aacggcctga aaccgcttgg ggccaacgag cggaaccggt gaggcgcgct
     3181 cgccccacac cggatccgtg aggacgcgct gcccgtcgat ctccaaaagc accgtcgaat
     3241 gtccgagcca agtggcgcgc agccccgttt caggcgcttt ttgccatggg gctcgagggt
     3301 ccagggtcgg cagtggggct acgggggagc gacgcccgcc gccgcacaga aactcaccga
     3361 tgctcggcat tttctccccg gtgcgcagcg cgggcaacac cggatgaacg tttcggaatg
     3421 agtcatcgac ccagttcttg gatgcacgca tccgttcgag ccgcgcgcca tttgcccgtt
     3481 taccgaatga gctgagcatg aacgtcggat gcatctacgc gggcgggagg acgacggtgt
     3541 cgcgaaactc gcggccgcgc gctaattcga cgtcaggcgt cgcgtacggg ttttgccaag
     3601 cggaagatcc cggcgacgtt gaatgctgcg aatgcgatca gcatggcttc gagcgccagc
     3661 acccaacgtg tgcccgacgg agactcacca tcgatcaaga gccaggcatc acgcggcaag
     3721 ccgtgccccg aagcctcggc cgccgtttcg agcgctctgt atcgaagccc cgcacctcgc
     3781 gccggtacga gccgtccaac gaacgaaccc ggcgcaacga agtgcgtttc atcggtatcg
     3841 cgcggcaccc gcagctcgac ccaaaccttc gggttgtcgg cgagcgcgct gagccgatag
     3901 ctgtcgctct cgagcggtcg cgaatagcgt acgacatctc ggtcgctgag cgctccttca
     3961 gcgcgcaccc aggagtttgg cgtgagcgcg gtcggcgcgc gcggcccgag ctccccgaga
     4021 tcggtcggtg ctccgccgcg cagggtgtag accaattcgc ctcgcaagcc gagcgcgaga
     4081 cgcagcgcaa agagcgccgt gacccccatc accacgaagg tgagtcggcg gaatgggcgc
     4141 cgcggtgggg ggagcgccgc cagatcgaca tcggtgctcg ctcgggtgct gtctgaggcg
     4201 ctcatccaga aaggtcctgt gactccgaca gctcggagct tctagggttc ccggagaatt
     4261 gcaagcggtg ggggacgagc gcgtcggagg tccggagctc gtcccgaaat tgtcgcgaaa
     4321 gccgaggtcc gcgccaccgc acaccttgcc cgacacagat ttgcgggcgt tcggccggcc
     4381 aagtgtgcgc gaaaacgccg gtgttctgcg cggcatccaa gcgtctggcg aggatttggt
     4441 gagcagctgt tcctcgtcca aaagtctggc tagcatgaaa ggccgctccc acgagtcgaa
     4501 cccccatggc cactgcgaac aagatcacgt gtccgtcctg cggctttcag aacacgcccc
     4561 cgctcgcgaa caaccgctgc gtctcgtgcg gcgcgaaaat tgaggacatc aagcggcagc
     4621 tgacgcgcca agaggaaatc gagcggcgct atcagcaaga gggcttcagc atgcagtggt
     4681 tcggcgtgtc gctggccatc atcatcgtca tgacggcggc gctcgtgatg gggctgccca
     4741 tggtcgtgcc ggcgctggat ttcgagggct cggcgggcat gctggtcgcc attccggtgt
     4801 ggtttttggg cggtctgctc atcgggctga tttcacccgg tcgcaccttc atcgagccgg
     4861 tcgtcgcagt gttcctggtc gcgatcccga cggcgtttct gctcttccag ggacagaccg
     4921 tgaagacgat gccggcgttc atgtacgccc tgatgtcggt gctcggcgtg ctgttcacgc
     4981 tcatcggttc gtacatcggt gagcgcattc agctcggtcc tccgccgaaa ccctccgagt
     5041 agctttcctc gatgagaact tcattcgcag agctcgctgc tctgctgctc tcggccctgc
     5101 tcgtctccaa ttgccgatcg tccgaggcgc gtgagcgcga ggcggaattc gcgcgcatcg
     5161 ggcactcgat cgatgcgttg cgcaacgctc ccaacgaggg gaaggcgcct ctcatccagg
     5221 cactcgagaa acaagagtgt cgcgagcgga gcgcctgcga cttgaaggag acgtgcatct
     5281 ctgcctaccg gctactcgac tcggcgcgcg gcgcgagtgc gaacgcgaag actctgctgg
     5341 ccaatcgcga tggcggcgcc aaaga
//
LOCUS       DASTKS010001002         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1499969, whole genome shotgun sequence.
ACCESSION   DASTKS010001002 DASTKS010000000
VERSION     DASTKS010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499969"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFQ35_21180"
     CDS             complement(<1..875)
                     /locus_tag="VFQ35_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933237.1"
                     /translation="MTRVELHPEELLERARKEIASPEELVELRAHLAACRACALEHAF
                     SADGDRASAPRADDDAVLARVRERTLRALAARTPRSAVRRVSRRLSILAAAALVLLIA
                     TYAVGSRLVGYLGLQRSEPPASSRSADSTRNGHVTISHPPDVSSSAPPVASPAPSAAP
                     TEPETAVPQKAQHTAARPARAAPEPVSSSEPSENAGELFARANLARRASNVTEAARLY
                     RELQRAFPGSTEARVSRVTLGRMLLDRLGDASGALLQFDSYLSNPSNRGLREEALIGR
                     ALALGRLGRRGEESAA"
     gene            complement(872..1603)
                     /locus_tag="VFQ35_21185"
     CDS             complement(872..1603)
                     /locus_tag="VFQ35_21185"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933238.1"
                     /translation="MEPDRPRATRSRYPAPAEAEVAGGAGIYGSALLDKEPAAAASAP
                     SALAHEMDRAAALVEAARQGDTAAIEALITLLAPRLLRAIRALMGPAHPDVEDLVQEV
                     LVDLMDALPSFRGESTLLHFAIRIATRKMTKKRRRAHAIRAWLEQWKRSEEPLSSSPA
                     SPREELVAERRRGLLRELLSELPEAQAEALVMRLSLGYSIEDIAEITRAPINTVRSRL
                     RLGKEALRARVANDPRWAELGVLES"
     gene            1896..3038
                     /locus_tag="VFQ35_21190"
     CDS             1896..3038
                     /locus_tag="VFQ35_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933239.1"
                     /translation="MCKASLSSSRMLLAVACSAFTALFSCTGENDSSSSGGSTAAGGA
                     ASGGSHAGGAPPAASGGAHAGGAPVGASGGAARGGTHAGGAPASTGGDASGPAGAAGE
                     GGAAGSGASSGAGWPNASNTGVPPGTQLTDYTTSCTITVNGTIVDSKRINCDLVIRAA
                     DVVIKNSKVKGLVYLDTDQPSSKDWSLTLQDSEVDGGPVQRAAVSDGNMTIIRANIYG
                     GETAVHCGEKALTCRIQDSWLHGQYMPDDVDWHLGGFQSNGGTNIQLIHNTVICDHPA
                     NRVGGGCTGDINLIPDWATISFVTVDRNFLGANTDASYCTYGGEKSTSDYPHADHIVY
                     TNNVFARGTNKKCGAFGPVTSFNSQGTGNVWTNNTWEDGGAIQPEN"
     gene            3061..>3419
                     /locus_tag="VFQ35_21195"
     CDS             3061..>3419
                     /locus_tag="VFQ35_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933240.1"
                     /translation="MRNPSNISRFAVLFTVAAGLSVTPLGCKNDDEPSNDEQTKGGSG
                     GGAGRAGSSGQGAALNGGSAGHIAGSNSGGAATNTSGGTATSDGGSTDGNAGRSTAPS
                     GGSGASGGARNSGGARNS"
BASE COUNT          643 a         1180 c         1098 g          498 t
ORIGIN      
        1 gccgcgcttt cctcaccgcg gcgaccgaga cgtccgagcg cgagcgcgcg cccaatcaac
       61 gcctcctctc gcaggccccg attggaagga ttgctcaagt agctgtcgaa ctgaagcagc
      121 gccccgctcg cgtcgccgag ccgatcgagg agcatgcggc ccagcgtcac gcgcgagacg
      181 cgcgcctcgg tagaaccggg aaaggcccgc tgaagctcgc ggtaaaggcg agccgcctca
      241 gtcacgttac tcgctctccg cgcgaggttg gctcgagcaa agagctcgcc cgcgttctcc
      301 gaaggctcgc tcgagctgac gggttcgggt gctgcacggg ccggccgggc tgccgtgtgc
      361 tgcgctttct gaggcacggc agtttcgggc tccgtcggag ctgcgctcgg cgcggggctc
      421 gcgaccggcg gcgccgaact cgaaacatcc ggcgggtgcg aaatcgttac gtgcccgttg
      481 cgtgtcgaat ccgcgctccg tgacgaagcc ggcggctcgg aacgctgcaa cccgaggtag
      541 ccgacgagcc tcgagccaac cgcgtacgtt gcgatcagca gcaccagggc cgccgcggcg
      601 agaatcgaga gacgacgcga tacgcgccgc accgcggacc gcggtgttcg cgcggcgagt
      661 gcccgcagcg ttcgctcccg cacgcgagcg agcactgcat cgtcatcggc cctcggcgcg
      721 ctggctcggt cgccgtcggc gctgaaagcg tgctccaacg cgcacgccct gcacgccgcc
      781 aggtgcgctc ggagctctac cagctcctcc ggcgaagcga tttccttgcg cgcgcgctcc
      841 aagagctcct cgggatgcaa ctcgacgcga gtcatgattc cagaactccc agctctgccc
      901 aacgcggatc gtttgcgacg cgtgcgcgca gcgcctcttt gccaagtcgc aggcggctac
      961 gcacggtatt gatcggcgcg cgcgtgatct cggcgatgtc ttcgatcgag taaccgagcg
     1021 acaagcgcat caccaaagct tcggcttggg cctccggcag ctcggagaga agctcgcgca
     1081 acagaccgcg gcgccgttcg gcaacgagct cttcgcgcgg ggatgcgggc gagctcgaga
     1141 gcggctcttc gctccgcttc cattgctcga gccacgcgcg gattgcgtgg gcacgtcgtc
     1201 tcttcttcgt cattttgcgc gtcgcgatgc ggatggcgaa gtggagcaac gtgctttccc
     1261 cacgaaacga cggaagcgcg tccatgaggt ccaccagtac ttcttggacg agatcttcga
     1321 cgtccgggtg cgcgggaccc atcaacgcgc gaatcgcgcg cagcagccgc ggcgcgagca
     1381 acgtgatcaa cgcctcgatc gccgccgtgt ctccctgacg cgccgcttcc acgagggcgg
     1441 cggcccgatc catctcgtgc gcaagcgccg acggggcact cgcagccgcc gcgggctcct
     1501 tgtcgagcag ggccgagcca taaatacctg caccaccggc cacctcggct tccgccggcg
     1561 cggggtagcg cgagcgagtg gctctcggcc gatcgggctc catggctcgg ctcgagtaga
     1621 aacgaggacg ggaaacggat caccggtttc gacggtgatc cgcgactttc aacgagccga
     1681 accggacaag ggtccggccc ccggtgggtg aaacggttcg cccctccccc ttccccttac
     1741 ctatcggcgg gggcccgcga aatctcacgc ccgagcgtga cccgaaaggt gccgtcgttc
     1801 ccacgagagc acgccacgcc ggaccgacga gagcacgcca cgccggaccg acgcgccgcc
     1861 gctcgagcca cgacgccacg gaggaagacc cctgcatgtg caaagcctcg ctttcatcgt
     1921 ctcggatgct gctcgccgtc gcgtgcagcg cctttaccgc gctgttctcc tgcacgggag
     1981 agaacgactc ttcgtctagc ggaggctcca cggccgcggg cggcgccgcg agcggaggtt
     2041 cgcacgccgg aggagcaccg cctgctgcaa gcggtggagc gcacgcaggc ggagcaccag
     2101 tcggtgcgag cggaggcgca gcgcgcggag gcacgcacgc aggcggagca ccagcttcaa
     2161 ccggaggcga cgcttccggt cctgctggcg ccgcgggtga aggcggagcg gcgggcagcg
     2221 gagcctcctc cggcgccggt tggccgaacg cctccaacac cggcgtaccg ccgggcacgc
     2281 aactcaccga ctacaccacc agctgcacca tcaccgtcaa tggcacgatc gtagacagca
     2341 aaaggatcaa ttgcgacctc gtcatccgcg cggctgacgt cgtcatcaaa aacagcaaag
     2401 taaaagggct cgtgtacctc gacaccgatc agccttcgtc caaagactgg tcactcacgt
     2461 tgcaagactc cgaggtcgat ggcggtcccg tgcagcgtgc ggcggtgagc gacggcaaca
     2521 tgaccatcat ccgcgccaac atctacggcg gagagaccgc ggtgcattgc ggcgaaaagg
     2581 cgctgacctg tcgcattcaa gattcgtggt tgcatgggca atacatgccg gacgacgtcg
     2641 actggcattt gggtgggttc caatcgaacg gcggaaccaa catccagctc atccacaaca
     2701 ccgtcatttg cgatcacccg gccaaccgtg tcggcggagg ttgcaccggc gatatcaacc
     2761 tgatccccga ctgggcgacg atctccttcg tcaccgtcga ccggaacttc ctcggcgcaa
     2821 acaccgacgc ttcttactgc acctacggcg gcgaaaagtc gacctcggac tacccgcacg
     2881 ccgaccacat cgtctacacc aacaacgtct ttgcacgtgg caccaacaag aagtgcggag
     2941 ccttcggccc ggtgacgagc ttcaacagcc agggcactgg gaacgtctgg accaacaaca
     3001 cttgggaaga cggcggcgcg attcaaccgg aaaactgaag tcgccaacag ggagaccgac
     3061 atgcgcaatc cgtccaacat ctcacgattc gcagttctgt ttaccgtcgc ggccggcctc
     3121 tccgtcacac cgctcgggtg caaaaacgac gacgagcctt cgaacgacga gcagaccaaa
     3181 ggaggctcag gcggcggagc aggccgcgct ggctcatcag gccaaggcgc cgccctgaac
     3241 ggcggctctg cgggacacat tgcaggttcg aacagcggcg gagcagcaac gaataccagc
     3301 ggaggaacgg ccacgagcga cggcggaagc accgatggaa acgccgggcg cagcaccgcc
     3361 ccgtcgggcg gaagcggtgc gtcaggcggc gcacgcaact caggcggcgc acgcaactc
//
LOCUS       DASTKS010001003         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1500402, whole genome shotgun sequence.
ACCESSION   DASTKS010001003 DASTKS010000000
VERSION     DASTKS010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500402"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFQ35_21200"
     CDS             <1..720
                     /locus_tag="VFQ35_21200"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933241.1"
                     /translation="GHGHPFERRSVSLVGDPARLDALRTELSRRDELRLETDASEESA
                     RRKLEVRAVEAVVIVRGSERRVLAASRAAIWGRGLAGLLHAELTETSIPERGYLHYLF
                     PGLLCSSVMFAGLYGMGYAMARYRQNAFLKKLATTPLRRELFVLAQVLGRAVLVVVQV
                     AMLLVCGLVAFSLPAPVGGMLSASISKIASGTPKNETALLRLRVTSKIRMRVRSHSAF
                     MTDYPRPSRARRSSRRTLLPK"
     gene            complement(653..1387)
                     /locus_tag="VFQ35_21205"
     CDS             complement(653..1387)
                     /locus_tag="VFQ35_21205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933242.1"
                     /translation="MSDPVVKVEHVSRDYGGVRALADVSFSIEGPGVVVILGPNGAGK
                     TTLLDLLAGLARPSAGAVYLFGARLDGSRYPRRDVGVVLQREFVPEHMSVREYAELFS
                     AIYGVEGGVEKIFSRAELSHRATVPVARLSGGEAQRLFIAAALVNEPRLLLLDEPSVG
                     LDPNHKRTLADLLRRLGRERTILMTTHDLSEAELVCDYCLFMVGASLRAHGPRDRLLA
                     SSGATTLEEAFFYYCGARVTAEGNLS"
     gene            complement(1457..2575)
                     /locus_tag="VFQ35_21210"
     CDS             complement(1457..2575)
                     /locus_tag="VFQ35_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933243.1"
                     /translation="MTLRFGLRRWLVIALALLASVAMGPEVVRLVQAKRFLTGLAAAK
                     PASADEALLEAELTLSGPHGPFRARVYRPAGGARAPGIVVAHGVHYQGIDERRLVPFV
                     RALARSGFVVLTPELSELADYRLTWDSVERIRAAVLFLSSRRDWTANERPGLLGFSFA
                     GGLALVAASEASLAGHLSYVVSVGGHHDLTRVLGFLLNDEIMTPSGRVHMRAHDYGLA
                     VVLYGHLEEFVPLAELPLAKNALRAWLHEDQKTARALGAALPQGTESARLFQLLAEHR
                     LQTLGPRLNALVARDRGELDALSPKGKLASIEVPIYLLHGAHDSVIPPSETTFAELEI
                     GARQHAALISPLIEHVEVGPSERLYDKLALLAFMAHLF"
     gene            2860..>3346
                     /locus_tag="VFQ35_21215"
     CDS             2860..>3346
                     /locus_tag="VFQ35_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933244.1"
                     /translation="MTLAVPFPWPLTCFEHPIAGEERAVERFIQTALETQEKERNAAE
                     DQRIRRAVHAKGHGLVVGNFHVQEVANADFRVGLFQKRKSYKVLLRPSNGATGVRPDR
                     KRDAHGLAMRVVLSEADLAELGDELLVVPESGRPIGHDFVLMDEPAFFVPNIGALADL
                     FA"
BASE COUNT          639 a         1077 c         1085 g          545 t
ORIGIN      
        1 gggcatgggc atcccttcga acggagatcg gtttccctcg tcggcgatcc cgcccggctc
       61 gacgcgctgc gcacggagct atcgcggcgt gacgaactgc ggctggaaac cgacgcttcg
      121 gaagagagcg cgcgacgtaa gctcgaggtg cgcgcggtcg aagcggtggt catcgttcgc
      181 ggctcggagc gccgggttct ggcggcgtcg cgggccgcga tttggggccg aggcttggcg
      241 gggctccttc acgcggagct cacggagacg tcgattccgg agcgcggtta cctgcattac
      301 ttgttcccgg ggctgctctg ctcttcggtg atgttcgcgg gcctctacgg catggggtac
      361 gcgatggctc gctaccgcca aaacgcgttt ttgaagaagc ttgcgacgac gccgctgcgt
      421 cgagagctgt tcgtgctcgc acaggtgctc gggcgtgcgg ttctcgtcgt cgtgcaggtg
      481 gcaatgctgc tcgtgtgcgg gctcgttgcg ttttcgttgc cggcgcctgt gggtggcatg
      541 ttgagcgcga gcatcagcaa gatcgcgagc ggcaccccga agaacgaaac cgcgctgctc
      601 cggcttcgcg tgacgtccaa aatccgcatg cgcgtgagaa gccacagcgc cttcatgaca
      661 gattaccctc ggccgtcacg cgcgcgccgc agtagtagaa gaacgcttct tccaaagtag
      721 tggcgccgga agaggcgagc agtcggtcac gcggaccgtg agcccgaaga ctcgccccga
      781 ccatgaacag gcagtagtca cagacgagct ctgcctcgct caagtcgtgc gtcgtcatca
      841 ggatcgtgcg ctctctgccg agccggcgta agaggtcggc gagcgttcgc ttatgattcg
      901 gatccaagcc gacgctcggc tcgtctagca acaggaggcg tggctcgttg accagcgccg
      961 cagcaatgaa gagccgctgc gcttcgccac ccgacaatcg agcgaccggc acggtggctc
     1021 gatgtgagag ctccgctcga gaaaagatct tctcgacgcc cccctcgacg ccgtaaatcg
     1081 ccgaaaagag ctccgcgtac tcgcgcacgc tcatgtgctc cggaacgaat tcgcgctgca
     1141 aaacgacgcc cacgtcccgg cgcgggtagc gcgaaccatc gagtcgcgcg ccaaacaagt
     1201 acacggcgcc agccgaaggc cttgccaacc ccgcgagtag atcgagcagg gtggtcttac
     1261 cggcgccgtt cgggccgagg atgacgacga cgccgggccc ttcgatactg aagctcacgt
     1321 cggcgagcgc gcgaacgcct ccgtagtctc ggctcacgtg ctccaccttc accaccgggt
     1381 ccgacatgtc gaaggtacgc tacgcgacgc ggcgcccggc tcgcaacaac tcgcgcgcac
     1441 caggcggcgc cgacgttcag aacaggtgcg ccatgaacgc gaggagcgct agtttgtcgt
     1501 ataggcgctc gctcggaccg acctccacat gctcgatcaa cggagaaatt agagctgcgt
     1561 gctggcgcgc tccgatttcg agctcggcaa aggtcgtctc actcggggga atgaccgagt
     1621 cgtgcgcgcc gtgaagcagg tagatgggga cctcgatcga ggcgagcttg cctttgggtg
     1681 agagcgcgtc gagctcgcct cggtcgcgcg caaccagcgc attcaagcgg ggcccgagtg
     1741 tttgcaagcg gtgctcggcg aggagctgaa ataggcgggc gctctcggtg ccttgtggaa
     1801 gggcggcgcc gagagctctc gctgtttttt gatcttcgtg cagccacgcc cgaagcgcat
     1861 tcttggcgag cggcaactcc gcgagcggaa cgaactcttc gaggtgacca tagagcacca
     1921 ccgcgagtcc gtagtcatgc gccctcatgt gaacgcgtcc ggacggcgtc atgatctcgt
     1981 cgttgaggag aaagccgagc acgcgcgtga ggtcgtggtg ccccccgacg ctgaccacgt
     2041 acgagagatg ccccgcgagc gacgcctcgg aggccgccac cagtgcgagc ccgccggcaa
     2101 agctgaagcc gagcaagcct gggcgctcgt tcgccgtcca atcgcgacga gacgaaagga
     2161 aaagcaccgc cgcgcgaata cgctccaccg agtcccacgt cagtcgataa tcggcgagct
     2221 cggagagctc cggcgtgaga accacgaacc cagagcgagc gagcgctcga acgaacggga
     2281 cgagccgacg ctcgtcgatg ccttggtagt gcacgccatg cgcaacgacg attcccggtg
     2341 cccgcgcgcc gcccgctggc cgatataccc gtgcacgaaa cggtccgtgc gggccgctca
     2401 aggtcagctc tgcttcgagc aaggcctcgt cggctgaagc aggcttcgcc gccgcgagcc
     2461 cggtaaggaa ccgtttcgct tgcaccaagc gcaccacctc gggacccatc gccacagaag
     2521 cgagcagcgc gagagcgatc actaaccaac ggcgcaaccc gaagcgcagc gtcacccgcc
     2581 tagtgtgacg ccaaaacagg cccgagctcc cctcccgacg atctacttac aacatgcgac
     2641 aatttcgagg atcgcgagac aagcgcggac accaccaaac cggtacgacg cgataggagg
     2701 aactttgatc tgcgaaagcc agcgaccgag cgaggcgcgt cgaagcttgc cgcgatcgag
     2761 aatccgagta ttgtgcgacg agtaacctca cgaacctcgc gcggataagc ttcgagggcc
     2821 gccatccgcg gaccttccga tgcgcgcagg gggagacgca tgaccctggc cgttccgttt
     2881 ccatggccgc tcacctgttt cgaacacccc atcgctgggg aagagagagc cgtcgaacgc
     2941 ttcatccaga cagcgctcga gacgcaggag aaggagcgca acgcagcgga ggaccagcgc
     3001 atccgccgcg cggtacacgc caaagggcac gggctcgtgg tcggcaactt tcatgttcag
     3061 gaggtcgcga acgcggactt tcgggtggga ctgttccaga aacgcaagtc ctacaaggtg
     3121 ctgctgcgac cttcgaatgg cgcaaccggc gtgcgtcccg atcgcaagcg cgacgcgcat
     3181 gggctcgcga tgcgggtcgt cctctccgag gctgacctcg ctgagctagg cgatgaactt
     3241 ctggtcgtgc cggagtccgg ccgccctatc ggtcacgatt tcgtactgat ggatgagcct
     3301 gcgttcttcg tgccgaacat cggcgcactc gcagacctct tcgcca
//
LOCUS       DASTKS010001004         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_381693, whole genome shotgun sequence.
ACCESSION   DASTKS010001004 DASTKS010000000
VERSION     DASTKS010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381693"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFQ35_21220"
     CDS             complement(<1..332)
                     /locus_tag="VFQ35_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933245.1"
                     /translation="MLVSPFVGNSLRLVAAQCALVSAFLGSMACGSSKSGDGASGGLA
                     NASGGDGPGPGAGTSATSGGATDPGSGGSSGAAKGGVGNSNGGSMSAANGGSANGGTT
                     SAANGGSPA"
     gene            423..1982
                     /locus_tag="VFQ35_21225"
     CDS             423..1982
                     /locus_tag="VFQ35_21225"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET9933246.1"
                     /translation="MVDPLPVARAGNADVSILPALANRHGCVTGATGTGKTVTLQRLA
                     EGFSSIGVPVFLADVKGDLSGLAAPGSATAKLEERLALLGLKGLEFRANPVTFWDVWG
                     EQGHPVRATISDLGPLLLARLLNLNETQEGVLTIAFKVADDNGLLLLDLKDLRALLGF
                     VAEDQASIKTQYGNVSAASVGAIQRALTQIEQQGGEHFFGEPMLDIHDLMQTDGSGHG
                     VINILAADKLLQTPRVYATFLLWLLAELFEQLPEVGDLEKPKLVFFFDEAHLLFKDAP
                     KALLEKVEQVVRLIRSKGVGVYFVTQSPLDIPETVLGQLGNRVQHALRAFTPRDQKAV
                     KAVAETMRANSKLDVRTSIMELSVGEALLSFLDEQGTPSVTERGWVIPPASRIGPLTR
                     EERSAAMQSSLVAGVYEQAIDRQSAFEELKKRAEGDSHKRGTRSPRGGAPPASAGAEP
                     SARGGAPAPAGGALDGMNEILFGRTGPRGGHHPGILDAFAKSAARSVGSSIAREITRG
                     IFGSLLGGKRR"
BASE COUNT          357 a          703 c          619 g          353 t
ORIGIN      
        1 gcagggcttc caccgttggc tgcgctcgtg gtgccgccgt tcgccgagcc gccgttcgcc
       61 gcgctcatcg agccgccgtt cgagttgccg acgccgccct tggccgcgcc ggagcttccg
      121 ccgctgccag gatccgttgc gccaccgctc gtcgcgctcg tgcccgcgcc ggggccagga
      181 ccgtcaccgc cggaagcgtt tgcgagacca ccgctcgcgc cgtcgcccga cttgctgctg
      241 ccgcaagcca tcgagcccaa gaaagccgag accagtgcac attgcgcggc caccagccgc
      301 agcgaatttc ccacgaacgg cgagactagc atgcccgttc agtatcgccg cgtcgctctc
      361 acggctcata tttcacgaca ttggtggacg cgcttaccgt ctgtaaggtt aagctcttaa
      421 ccatggtcga tcccctacct gtcgcgcgcg cagggaacgc cgacgtttcc atcttaccgg
      481 cgctcgccaa ccgccacggc tgcgttaccg gtgcgaccgg caccggaaag acggtcacgc
      541 tccagcgcct cgccgaaggc ttttcgagca tcggggtgcc ggtgttcctc gccgacgtca
      601 aaggcgatct cagcgggctg gcggccccgg gcagcgcgac cgcaaagctc gaggaacggc
      661 tcgcattgct gggactgaaa ggcctcgagt tccgcgcgaa cccagtcaca ttttgggacg
      721 tctggggcga acagggccac cccgtgcgcg ccacgatttc ggacttggga ccgctcttgc
      781 tcgcgcggct cttgaacctg aacgagacgc aggaaggcgt cctgaccatt gctttcaaag
      841 tcgccgacga caacggcctt ctcctgctcg acttgaagga tttgcgcgcg ctgctcggct
      901 tcgtcgcgga ggaccaagcg agcatcaaga cgcaatacgg caacgtctcc gccgcgtcgg
      961 tgggcgccat ccagcgcgcg ctcacccaaa tcgagcagca gggcggcgag cacttcttcg
     1021 gcgagccgat gctcgacatt cacgacctga tgcagacaga cgggtcggga cacggcgtga
     1081 tcaacatctt ggcggccgac aagctcctcc aaacaccgcg cgtctacgcc acgttcttgc
     1141 tgtggctgct cgccgagctc ttcgagcaac tgccggaagt cggcgacttg gagaagccga
     1201 agctcgtctt cttcttcgac gaggcccacc tgctcttcaa ggacgcgccg aaggctctgc
     1261 tcgagaaggt agagcaggtc gttcgcttga tccgctcgaa gggcgtcggt gtctacttcg
     1321 tcacgcagag cccgctcgac attccggaga ccgtcttggg ccagctcggc aaccgtgttc
     1381 agcacgcact ccgcgctttt acgccccgag atcaaaaggc ggtcaaggca gtggcggaga
     1441 cgatgcgggc gaactccaag ctcgacgttc gaacctccat catggagctc tcggtcggtg
     1501 aagcgctgct ctccttcctc gacgaacaag gcaccccgag cgtgaccgag cgcggctggg
     1561 tgattccccc tgcgagtcgc atcggcccgc tcactcggga agagcgcagc gcggccatgc
     1621 aatcgtcgtt ggtggccgga gtctacgagc aagcgatcga ccgccagtcg gcgttcgaag
     1681 agctgaaaaa gagagcggag ggcgactctc acaaacgcgg aacccgatca ccgcgcggag
     1741 gcgctccccc tgcctccgct ggcgcagaac cttccgcgag gggcggagcg cccgctcccg
     1801 ctggcggagc cttggatggc atgaatgaaa tcctattcgg ccgcactggt cctcgcggtg
     1861 gacatcaccc cggcatcctc gacgcattcg ccaagagcgc agcgcgctcc gttggctctt
     1921 caatcgcccg tgagatcacc cgcggcatct ttggctccct ccttggagga aaacgccgtt
     1981 gaacgactta cgccaaccga ccgtcgtccg tttcgtagcg gcgtgactcc ta
//
LOCUS       DASTKS010001005        11944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1946938, whole genome shotgun sequence.
ACCESSION   DASTKS010001005 DASTKS010000000
VERSION     DASTKS010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11944
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946938"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFQ35_21230"
     CDS             complement(<1..185)
                     /locus_tag="VFQ35_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933247.1"
                     /translation="MSEPQNKLRQNAAPVSKDTGPRLTHAFDGSAQHVEPEIVGADDS
                     LPGGTRLKIVTIAPARGS"
     gene            509..2101
                     /locus_tag="VFQ35_21235"
     CDS             509..2101
                     /locus_tag="VFQ35_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933248.1"
                     /translation="MGFPLDLALRYMRSKKRAFVSVGTLFAILGVALGVAALATVISV
                     TGGFRQQFRDKVLGVNAHVLVLKYSSDFREYRKIMDRMKSVPGVTGIAPFVINPMMVT
                     HADRTATGVLLKGVDPARVAAVLDLPKHIIRGSLAGLRLPSAKPPERRRQSLSDDIAT
                     DFGGKGGVAVPLGADDAPSRTRSEGTPKKAVLGEIEKLLKKDAAVDAPRASDDAAENK
                     AEAAVPQLPEGSIVPEGGYKSVLPDDDAVPAELDPDPCANKETVARLPGIVLGTTLAK
                     NLGVDLGGCVQVTSPTIGYSFSKGAIRAPVAKQFRVTAIFEAGFDQYDSKLVYTDLYE
                     AQAFYDAGDSVTGIEMKVSNIDDAKKIAADIDQRLGSGIYHTMDWEELNHGLFTALRI
                     QQILMSLVLALIIVVAAFTVIATLIMVVLDKKREIAVLKAMGASDGALLRAFVYQGAL
                     IGVVGTAAGLVLGYATCQGLLKYAVPLDPKVYFIPRLPVQVQAIDFAMVGLFAILVCV
                     LATVWPAVHASRLRPAEAFREN"
     gene            2174..3622
                     /locus_tag="VFQ35_21240"
     CDS             2174..3622
                     /locus_tag="VFQ35_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9933249.1"
                     /translation="MGFPLELALRYMRSKKRAFVSVGTLFAILGVALGVAALATVISV
                     TGGFRQQFRDKVLGVNAHVLVLKYSGDFREYRKIMDRVKSVPGVTGVAPFVINPMMVT
                     CGDRTATGVTLKGVDPARVATVLDLPKQIVRGTLAGLRLPDARPPERRRRSFSDDAAP
                     EKESPGEPTQGKLAAEQLPEGSIVPEGGYKSVLPDEDTVPAALDPDPCANPENVAKLP
                     GIVLGTTLAKNLGADVGGCVQVTSPTIGYSFSKGAIRAPVAKQFRVTAIFEAGFDQYD
                     SKFVYIDLYESQAFYEAGDSVTGVEMKVSNIDDAKSIAARIEELLGNGIYHTMDWEEL
                     NHGLFTALRIQQILMSLVLALIIVVAAFTVIATLIMVVLDKKREIAVLKAMGASDGAL
                     LRSFVYQGALIGIVGTAAGLVLGYATCQGLLKYAIPLDPKVYFIPRLPVQVQATDFAL
                     VGVFAIAVCVLATLWPAVHASRLRPAEAFRES"
     gene            complement(3704..4417)
                     /locus_tag="VFQ35_21245"
     CDS             complement(3704..4417)
                     /locus_tag="VFQ35_21245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933250.1"
                     /translation="MQGIVYTFEDLRELSTLLESSGEDQDLALPCRCGVCEGEWLLAT
                     FSAGEESTCVPGRICDRGQGLRLAFEARDWERLCEFASGDARPSIPPPCDAAPVGAVS
                     APPGSSALLVDADAGVSSIVRAMLEACGVATDTVHGAEDALDLLRRRRFDLIVVEPTL
                     NGMSGLELCERLRADSELCATPVLVLASHTGDRDLRELKRCGASDFVGKPFRAHELRA
                     RAIGLIQQGRAGAPSARRA"
     gene            complement(4570..5253)
                     /locus_tag="VFQ35_21250"
     CDS             complement(4570..5253)
                     /locus_tag="VFQ35_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933251.1"
                     /translation="MTALKLGVRTALPLESARGLVQTLCAVVLGCALTAWLERVASPS
                     HAVDRALVRDAFGLWLPLAAYAAAERIGKKGRLELVIAALPRYGLSRRASLAGAVLAL
                     GGVLAIVGACCGAISVVVAHEDGSSALLSDLVASTWIGALGGVSYAVWLGFASGFGRR
                     GRRGTFLILDLIFGSSSRFWALPFPRAHLRNLLGGTPLVGLSQAASVGLLAVLIGVVF
                     ALTLARVPD"
     gene            complement(5250..6152)
                     /locus_tag="VFQ35_21255"
     CDS             complement(5250..6152)
                     /locus_tag="VFQ35_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933252.1"
                     /translation="MAGESARLTELWLSDVFDRRVGPLTLRLGVGLTSILSDDAESLG
                     ALAELLVGLRAPKRGTVLLDNLALSGSAPARARVASVLREEALLPAPTVSAALSQALA
                     LRGAAGSAEACLAALGLERFLPVAPSALEPMELRAIAFALAAALAERSALLVLHEPFA
                     LVPLVPARVIVETCRRHARERIVVSLLSELDRGLELGERCLVLERGKPSPFGALAAGP
                     TTTLSVRSPQAARLAELSQQFAEVSYVEVLGDEVSITTSDPVAFSRNLVSAAVEADLD
                     ITGVALPSASLHDQLWLRSLGSAR"
     gene            complement(6128..6766)
                     /locus_tag="VFQ35_21260"
     CDS             complement(6128..6766)
                     /locus_tag="VFQ35_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933253.1"
                     /translation="MARPAALVRLQLRRLSERPGMRAARLYSYGILASFGLLALLSRS
                     GPPGLTKLASSALESASLVVAGLSAWACAADLSREDRRDGVAALAGVHGVSARTLERW
                     RTLSAVVRTGVAIAAPALGVVALGVLRNGALLWALALSFGIVAYGLVLATSLVPLARL
                     AALVGKGQGKLLLLACVATPELLRLAGAPVPSWSSVFGGLLHELEWLGRALA"
     gene            complement(6782..7702)
                     /locus_tag="VFQ35_21265"
     CDS             complement(6782..7702)
                     /locus_tag="VFQ35_21265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9933254.1"
                     /translation="MAQAKQRPSSAPRTLPASENQKLTEEVMRRIKAPHRPALVVATG
                     EQAGTRVVLDRSITIGRDPVAELVLADALVSWHHARVEDRGDSWTLIDLDSTNGVSVN
                     GERCTEWRLNPSDRIVVGSTAILFELQDGLSQALNENVENLLNVDDLSGLLVRRKFDT
                     ELDRLLEFARRQGEPIALLVMDLDGIKKINDTHGHLFGAYTIGEAGRLIARVISGAGV
                     GCRFGGDEYLAALPGHDVSDATHVAERILETIHAHHFEKDGIRLHPGISIGVAAFPRN
                     ANDAQGLFQAADKAMYNAKQSGKNRVAVTP"
     gene            complement(7768..8652)
                     /locus_tag="VFQ35_21270"
     CDS             complement(7768..8652)
                     /locus_tag="VFQ35_21270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9933255.1"
                     /translation="MKLEVLFTAALSIACGAAPLPKQAIELNTRGARALAEGDLESAD
                     SALSVALEYSPHFVDALVNLGLVECQRGNFERARTLLLRARRLNPDVAQPHHALGVLA
                     ERERRPDLASEHYWEALHVDPGFAPARENLGRLQFDAGMFDAASLTFKKLIQVAPNAP
                     SGYAGLSEALLRLGRTNEAQEVVARASERFPNDPGIALLGARCAIRRGEFERAQADLE
                     QLSERGDEYAAAALGWLATLYLARGEADVALQVANRSLGLVPDEPVALYAAAQALAAL
                     GDRRAPAWLERRRKLGQR"
     gene            8788..9597
                     /locus_tag="VFQ35_21275"
     CDS             8788..9597
                     /locus_tag="VFQ35_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933256.1"
                     /translation="MSTEAESAPAKSPSNAPNAPRKKKKKVVKRKLTRPLTEQEINAP
                     DMQTMVMVGCLGVMTIILWAWAHAGCNYHPPRETRRPRDVTTAELTREPKDAAIEFQH
                     RLLTRNFKGALEIAAGSAVDAVKQEQAKCDANRAACAQEKSRLGDAVTSATVLERTPA
                     NAKVRATSYHLPNGNQTFLNLVERDATGWKVTARVPDGPGATLPAPILPPPAPLHFNV
                     TPAGSGGGIRLMPPDHPGASSPPSAPAAPGPASSPAAPKPALVAPAPASSK"
     gene            complement(9626..10135)
                     /locus_tag="VFQ35_21280"
     CDS             complement(9626..10135)
                     /locus_tag="VFQ35_21280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9933257.1"
                     /translation="MKPWLSRLIDYALYAALAVVALKFVARKFSGPGEGTRASAFELP
                     IVDRPGEHFRLQDHAGKPVLMEVFASWCGACRRSAPAVVESFERHHQKGVEFVGISVD
                     SSSEDAARVKTDWRIPYDVALDDGRVAKNYGIEVLPTFVLIGKDGTVRHVSTGSVSPS
                     DIDRWLGEL"
     gene            complement(10132..10833)
                     /locus_tag="VFQ35_21285"
     CDS             complement(10132..10833)
                     /locus_tag="VFQ35_21285"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9933258.1"
                     /translation="MTTSNTDPPDLPWPKPAAPSLECSRAIREGCTRGLSHKRCVPAS
                     HRLLGSLALSMGVFALLAWLTRANPRIDGTFRAALIGAAGWGIVQAMILWVGLARPPG
                     KRFSARVRLVLAVLTPVIFLGYVAHAAPEWVSFMEFSHGDRAAHAIRCTAAGLAFSAV
                     ISGGVLLLWRGTDPLTPGLTGALVGLVGGLAGGLTIGVACPSQEGWHACFSHGLGALA
                     FAGIGWAAGRRLLVP"
     gene            complement(10830..11408)
                     /locus_tag="VFQ35_21290"
     CDS             complement(10830..11408)
                     /locus_tag="VFQ35_21290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9933259.1"
                     /translation="MPGVVLGRQLHLPSARPEELMEAYVDGDADAFDELYRRLAPNLF
                     GYLLRLTRQRERAEDLLQVTFAKVHRARGSYLRGAPVLPWMLAIARRSFLDERRHAST
                     RTEDLSFDGNLPEPRPEDAALPADLSAALERALDELPEGYREAIQLTKISGLSIAEAA
                     DVLGTTETAVKLRVHRGYTQLRKELERFNRTV"
     gene            complement(11596..>11944)
                     /locus_tag="VFQ35_21295"
     CDS             complement(11596..>11944)
                     /locus_tag="VFQ35_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9933260.1"
                     /translation="LDAVADVIRNDKQLAGRYFQVAGHTDNKPLVGGRFGDNWGLSVM
                     RAREVLIYLIAPLGGKTAGGGLDPLRLHAAGYGETDPVMKNDTDDGRQQNRRVELVLM
                     PDVEEMLDLKSLI"
BASE COUNT         2124 a         3874 c         3992 g         1954 t
ORIGIN      
        1 gaaccgcgcg ctggcgcgat ggtcacgatc ttcaaacgcg tgccgccggg cagagaatca
       61 tccgctccga ctatctcggg ctcgacgtgc tgagcgctgc cgtcgaacgc atgcgtgagc
      121 cgcggccctg tatctttcga cacgggcgct gcgttctgtc ggagcttgtt ctgcggctcg
      181 ctcatcgggt cctggggatt gattcgggtc attctgcggg caagctgtgt gcgacgttaa
      241 gctgaactgt cgttcaccga ccagctgcgc ggctcatcat tatgatgcaa gagtgagacc
      301 cgtgcttggc gcattgaaat cattcagcaa ttttgtccac aggaaggccc gcgctccgca
      361 aaccttgcga cccggcagca ctcactgcca ggttcctgac gttggccggc gggcctaggc
      421 tgtgagcggc cgcgcgcggg gccgtgggag ccatcgcgtg cccgccttta tcaccgatga
      481 gagaggagct gatggtaaac ccgcggcgat gggtttcccg ctcgatctcg cgctgcgcta
      541 catgcgttcg aagaagcgcg ccttcgtttc cgtgggcacg ctgttcgcga tcttgggggt
      601 cgcgctcggt gtggccgcgc tcgcgacggt gatcagcgtc acgggaggtt ttcgccagca
      661 gtttcgggac aaggtgctcg gggtcaacgc gcacgtgctg gtgctgaagt actcgagcga
      721 ctttcgcgag taccgcaaga tcatggaccg catgaagagc gtgccgggcg tgaccggcat
      781 cgcgcccttc gtgatcaacc ccatgatggt aactcacgcg gatcgcacgg cgacgggcgt
      841 gttgttgaag ggcgtcgatc ctgcgcgcgt cgcggcggtg ttggatttgc cgaagcacat
      901 cattcgcggg tcgctcgcgg gactgcgctt gcccagcgcc aagccgccag agaggcgtag
      961 gcagtcgctc tcggacgaca ttgcgaccga cttcggcggc aagggcggcg tcgccgttcc
     1021 tctgggggcc gatgacgcgc cgtcgagaac tcgaagcgag ggcaccccga agaaggcggt
     1081 gctcggtgag atcgagaagc ttttgaagaa ggacgcggcc gtcgacgctc cacgcgcctc
     1141 cgacgacgcc gccgaaaaca aggcagaagc agcggtcccg cagcttcccg agggcagcat
     1201 cgtgccggag ggcggttaca agagcgtgtt gcccgacgac gacgcggtgc cggccgagct
     1261 cgatccggat ccgtgcgcca acaaggagac ggtggccagg ctaccgggca tcgtgctcgg
     1321 gacgacgctc gccaagaatc tgggcgtcga tctcggtggc tgcgttcagg tcacctcgcc
     1381 gacgatcgga tactcgtttt cgaagggtgc gatccgcgcc cccgtcgcga agcagttccg
     1441 cgtcacggcg atcttcgagg ccggcttcga tcagtacgac tcgaagctcg tctacaccga
     1501 tttgtacgaa gcgcaggcct tctacgacgc gggcgatagc gtgaccggca tcgagatgaa
     1561 ggtgagcaac atcgacgacg ccaagaagat cgcggcggac atcgaccagc gcttgggcag
     1621 cggcatctat cacacgatgg attgggaaga gctgaaccac ggcctattca ccgccctacg
     1681 cattcagcaa attctgatga gcctcgtgct cgcgctcatc atcgtggtcg ccgctttcac
     1741 cgtgatcgcg acgctcatca tggtggtgct cgacaaaaag cgagagatcg cggtgctcaa
     1801 agcgatgggc gcctcggacg gagcgctgct gcgagcattc gtgtaccagg gagcgctgat
     1861 cggcgtcgtc ggtaccgccg cgggcctcgt cctcgggtac gcaacgtgcc agggattgtt
     1921 gaagtacgcg gtgccgctcg accccaaggt ctacttcatt ccgcgcctgc cggtgcaggt
     1981 gcaggccatc gacttcgcga tggtcggcct cttcgcgatc ctcgtttgcg tgctcgcgac
     2041 ggtgtggccg gcggtgcacg cgtcgaggct ccgccccgcc gaagccttcc gcgagaactg
     2101 agccgtatgc gcgtctcgtg aagccccggc gcgatcgatc gccgcggtcg gcttccgatg
     2161 ctagaccgct cgcatgggtt ttccgctcga gctcgccctg cgctacatgc gctcgaagaa
     2221 gcgggccttc gtctcggtcg ggacgctgtt cgccattttg ggtgttgcgt tgggcgtcgc
     2281 tgcgctcgcg acggtgatca gcgtgacggg gggcttccga cagcagtttc gcgacaaggt
     2341 gctcggggtc aacgcgcacg tgctggtgct gaagtattcg ggagactttc gcgagtaccg
     2401 gaagatcatg gatcgagtga agagcgtgcc cggcgtgacc ggcgtcgctc cgttcgtgat
     2461 aaaccccatg atggtgacgt gcggtgatcg caccgcgacc ggcgtcactc tcaaaggagt
     2521 cgaccccgcg cgcgtcgcga cggtgttgga cctgccgaag caaatcgtgc gcggcacgct
     2581 cgctgggctg cggctgcccg acgcgcgccc gcccgagcga cgtcgtcgtt cgttttcgga
     2641 cgacgcggcg cccgaaaagg aatcaccggg cgaaccaacg cagggcaagc tcgcggcgga
     2701 gcagctgccg gagggcagca tcgtgcccga aggcgggtac aagagcgtct tgccggacga
     2761 agatacggtg ccggccgcgc tcgatccgga tccctgcgcc aaccccgaga acgtcgcgaa
     2821 gctgcccggg atcgtgctcg gcacgaccct cgcaaaaaac ttgggggcgg acgtcggtgg
     2881 ctgcgttcag gtcacgtcgc caacgatcgg ctattcgttt tcgaagggcg caattcgtgc
     2941 gccggtggcg aagcagtttc gcgtgacggc gatcttcgaa gccggtttcg accaatacga
     3001 ctcgaagttc gtctacatcg acttgtacga gtcgcaagcg ttctacgagg ctggcgatag
     3061 cgtgactggc gtcgagatga aggtgagcaa catcgacgac gcgaagagca tcgcggctcg
     3121 aatcgaggag ctgctcggca atggcatcta tcacacgatg gattgggaag agctgaacca
     3181 tgggctcttc acggcgctcc gcattcaaca gatcttgatg agcttggttc tcgcgctgat
     3241 catcgtcgtg gcggcgttca cggtgatcgc gacgctcatc atggtcgtgc tcgacaagaa
     3301 gcgcgagata gcggtgctca aagcgatggg cgcctcggac ggagcgctgc tgcgctcctt
     3361 cgtgtaccaa ggtgcgctca tcggcatcgt cggtaccgcc gcgggcctag tgctcggata
     3421 cgccacttgc caagggctct tgaagtacgc gatcccgctc gatccgaagg tctacttcat
     3481 cccgcggctt ccggtgcagg tgcaagcgac cgacttcgcc ctggtgggcg tattcgccat
     3541 cgcggtgtgc gtccttgcga cgttgtggcc agcggtgcac gcctcgaggc ttcgccccgc
     3601 cgaagctttc cgcgagagct gagcccgtcg tcgttcgaac tcgccagccc ggccgctttg
     3661 agccgcgcac cgcgacgacg caggggccgg ggagggtgat tcttcaggcg cggcgagccg
     3721 atggggcgcc ggcgcggcct tgctgaatga ggccgatggc gcgagcgcgg agctcgtgcg
     3781 cacggaatgg ctttccgacg aagtcgctcg caccgcagcg cttgagttcg cgcaaatcgc
     3841 gatcgccagt gtgcgaggcc agcacgagca ccggtgtcgc gcagagctcg gagtcggcgc
     3901 gcagtcgttc gcacagctcg agcccgctca tgccgttcaa cgtgggttcg accacgatca
     3961 ggtcgaagcg gcgacggcgg agcaagtcga gcgcgtcttc ggcgccgtgc accgtgtcgg
     4021 tcgcaacgcc gcacgcctcg agcatggcgc gcacgatgga cgagacgccc gcgtccgcgt
     4081 cgacgagcag ggcagacgag cctggcggcg cgctgacggc gccgaccgga gccgcgtcgc
     4141 acggaggagg gatgctcgga cgggcatcgc cgctcgcgaa ctcgcacagg cgctcccagt
     4201 cgcgcgcttc gaaggcgagc cgtaatccct gaccgcgatc gcaaatgcga ccaggaacac
     4261 aggtgctctc ttcaccggcc gagaacgtcg cgaggagcca ctctccttcg cacacgccgc
     4321 accgacacgg gagcgcgagg tcttggtcct cgccgctcga ttcgagcaat gtcgacagct
     4381 cccgcaaatc ttcgaacgta taaacgatgc cttgcaagcc ccgcgagcct actcccgagg
     4441 ttcgaaccgt gccagtttgg aacaagcggg agacccgatt tattgcgacc ggtttcactt
     4501 ttggatacgc cagattcact cgactgctga tgtgcgcccg ttacgccagc aagagtcgtt
     4561 ccaaccgcgt caatctggca ctctcgcgag ggttagcgcg aaaactacgc cgatgagtac
     4621 ggcgaggagc ccgacgctcg ctgcctgtga aaggccgact aatggggtgc caccgagcag
     4681 atttcgcagg tgagcccgtg ggaagggcag agcccaaaag cggctcgaac tgccgaaaat
     4741 cagatccaaa atgagaaagg tgccgcgccg tccccttcgc ccgaaccccg acgcgaaccc
     4801 caaccaaacc gcgtaggaga cacctccgag cgcgccgatc caggtgcttg ccacgagatc
     4861 gctcagcagc gccgagctgc cgtcttcgtg agcgacgacc accgagatgg cgccgcagca
     4921 tgctcctacg atggccaaca cgcctcctaa cgccaagacc gccccggcaa gggatgctct
     4981 gcgactcagc ccgtagcgcg gtaacgcagc gatcacgagc tcgaggcggc ccttcttccc
     5041 aatccgttcg gcggctgcat acgccgcgag cgggagccag agcccaaagg catctcggac
     5101 gagcgctcga tcgacggcat ggctcggaga ggcgacgcgc tctaaccacg cggtcagcgc
     5161 gcatccaagg actaccgcgc agagcgtttg aacgaggccg cgagcgctct cgagcggtag
     5221 cgcagtgcgc acgccgagct tcagtgcggt catcgagcgc tccccagcga gcgaagccag
     5281 agctggtcgt gaagcgaagc gctcggcagc gcgacgccgg tgatgtcgag gtcggcttcg
     5341 acggcggcgc tcacgagatt gcgcgagaag gcaacgggat cggaagtggt gatcgagact
     5401 tcgtcgccga gcacctcgac gtatgacact tcggcgaact gctgcgagag ctcggccagg
     5461 cgtgccgcct gcgggctgcg aaccgagagc gtcgtcgtgg gccccgcggc gagcgctccg
     5521 aacgggctcg gcttaccgcg ctcgaggacg agacaacgct cgccgagttc gagtccgcga
     5581 tcgagctcgg acaagagcga cacgacgatg cgctcgcgcg cgtggcgccg acaggtctcc
     5641 acgatgacgc gagcggggac gagcggcacg agcgcgaaag gttcgtgcaa gacgagcaac
     5701 gcagatcgct cggcgagcgc ggccgcgagc gcgaacgcaa tcgcgcggag ctccatcggc
     5761 tcgagcgcac tcggcgctac ggggagaaac cgttcgagtc cgagcgcggc caagcacgct
     5821 tcggcggagc ccgccgcgcc ccgcagggcg agcgcctggc tcagcgccgc gctcaccgtc
     5881 ggcgcaggca gcaacgcttc ttcgcggagc accgaggcga cccgagcgcg cgccggcgcc
     5941 gagcccgaga gcgcgaggtt gtcgagcagc acggttccgc gcttgggcgc tcggagccct
     6001 acgaggagct cggcgagcgc tcccagtgac tccgcgtcgt cgctcagaat cgaagtgagg
     6061 ccgacgccga gccgcagcgt cagcggcccg acgcgccgat cgaaaacgtc actcaaccag
     6121 agctcggtca agcgagcgct ctccccagcc actcgagctc gtgcagcaag ccgccaaaga
     6181 cgctggacca gctcggcacg ggcgcgcccg cgagtcgaag gagctctggc gttgccacgc
     6241 atgcgagcag cagcagcttg ccctgcccct tgccgacgag cgccgcgagc cgagccagcg
     6301 gcacgagcga agtcgcgagc accaagccat aagccacgat gccgaacgac agcgcgagag
     6361 cccagagaag cgcgccgttt cgcagcactc cgagcgcgac gacgccgagg gcaggggcgg
     6421 ctatcgcgac gccggtgcgc acgacggcgc tcagcgttcg ccagcgctcg agggttcgtg
     6481 cgctcacgcc gtgcactccg gccaaggccg cgactccgtc tcgacggtct tcgcggctga
     6541 gatcggcggc acatgcccac gccgaaagcc cggcgacgac gagcgacgcg gactcgagcg
     6601 cgctcgatgc gagcttggta aggccgggcg ggccggagcg cgagagcaac gcgagcagcc
     6661 cgaagctcgc gaggatgccg taggagtaca gccgcgccgc ccgcatgccc gggcgctcgg
     6721 agagccttcg aagttggagt cgaacgagag cggccggacg cgccacgctg aattccgatc
     6781 gctaaggagt gacagcgacc ctatttttgc ccgactgctt ggcgttgtac atggccttgt
     6841 cggcggcttg gaacaggccc tgggcgtcgt tcgcgttgcg agggaaggca gcgaccccga
     6901 tcgaaatccc ggggtgcaga cggatcccgt ctttttcgaa gtggtgagcg tgaatcgtct
     6961 ccaaaatgcg ctcggcgacg tgcgtggcgt cgctcacgtc gtgccccggc aaagcggcca
     7021 agtactcgtc tccgccgaag cggcagccga ccccggctcc cgaaatgacg cgagcgatga
     7081 gtcgacccgc ctcaccgatg gtgtaagcgc cgaataggtg gccgtgcgtg tcgttgatct
     7141 tcttgatgcc gtcgaggtcc atcacgagga gcgcgatggg ctcgccttgg cgtcgcgcaa
     7201 actcgaggag ccggtcgagc tcggtatcga acttccggcg tacgagcagg ccagagaggt
     7261 cgtctacgtt gagcaggttc tcgacgttct cgttgagagc ctgtgagagc ccgtcttgga
     7321 gctcgaacaa gattgcggtg ctcccgacga cgatgcggtc gctcgggttc aggcgccact
     7381 cggtgcaacg ctcgccgttc accgagacgc cgttcgtgct gtccagatcg atgagcgtcc
     7441 agctgtcgcc ccgatcttcg acgcgcgcgt ggtgccacga aacgagtgcg tcggcgagga
     7501 cgagctccgc aaccgggtcg cgcccgatcg tgatcgaccg atcgagcacg acgcgtgtgc
     7561 cggcctgttc acccgtcgcg accaccaacg ctggccggtg aggcgccttg atgcggcgca
     7621 tgacctcttc ggtgagcttt tggttctcac tcgccggtag cgtgcgtgga gcactactag
     7681 gacgctgttt cgcttgagcc aatgattgac cctaccacga cggcttcgcg cttgcctctg
     7741 acgctggtga agggattggt agggatttca gcgctggccg agctttcttc ggcgctcgag
     7801 ccaggccggc gcgcggcggt caccgagcgc ggccagagct tgggccgcgg cgtagagcgc
     7861 gacgggttcg tcgggtacca aacccaggct gcggttcgcc acttgcaaag caacgtcggc
     7921 ttcgcctcga gcaaggtaga gtgtggcgag ccaaccgagc gcggctgcgg cgtattcgtc
     7981 gcctcgctca ctcaactgtt cgagatccgc ctgcgccctc tcgaactcac cccgtcgaat
     8041 cgcgcagcgc gcgcccaaga gagcaatacc cggatcgttc gggaagcgtt cgctcgcacg
     8101 cgctacgact tcttgtgctt cgttcgtcct cccgagtcgc agcaacgcct cgctgagtcc
     8161 cgcgtaaccg ctcggtgcgt tcggagcgac ttggatgagc tttttgaacg tcagcgaggc
     8221 ggcgtcgaac atcccggcgt cgaactgtaa gcgtcccaag ttctcacgcg cgggagcaaa
     8281 gccgggatcg acgtgcaggg cctcccagta atgttccgat gcgaggtcgg gtcggcgctc
     8341 gcgctcagcg agcacgccga gtgcatggtg gggctgcgcg acgtcggggt tcagccttcg
     8401 cgcgcgcagc aacagcgtgc gtgcccgttc gaagttgccg cgctggcact cgaccaggcc
     8461 gaggtttacg agcgcgtcga cgaagtgagg gctgtattcg agcgcgacgc tcagggccga
     8521 gtcggcgctc tcgagatctc cttcggccag cgcgcgagct ccgcgggtgt tcagctcgat
     8581 ggcctgtttg ggcagcggag ccgctccgca agcaatggaa agagccgcgg tgaatagaac
     8641 ttcgagcttc acggggtctc ggtcggacgg cgcgcgcgag gagttgcgta aaaccgttgc
     8701 cgaccgcgcg gccgatcaca cgcttggtgc ccccaccgcg cgagtcaggg ttgcgtgtcg
     8761 gcgcgcccga aggaatgctt gcgccccatg tccaccgagg ccgagtccgc gccggctaag
     8821 tcgccgtcga acgcccccaa cgcgccgcga aaaaagaaga agaaggtcgt gaagcgcaag
     8881 ctcacgcgtc cgctaacgga gcaagagatc aacgctccgg acatgcagac catggtgatg
     8941 gtgggctgcc tcggcgtgat gacgatcatc ttgtgggctt gggcgcacgc gggctgcaac
     9001 taccaccctc cgcgtgagac gcgtcgtccg cgcgatgtga cgacggcgga gctcacgcgc
     9061 gaaccgaagg acgcggccat cgagtttcag caccggctgc tgacgcgcaa cttcaaaggc
     9121 gcgctagaga tcgcggcggg gtccgcggtc gacgcggtga agcaagagca ggcgaagtgc
     9181 gacgcgaatc gcgcggcctg cgctcaagaa aagtcgaggc tcggagacgc cgtcacgtcg
     9241 gcgacggtcc tcgagcgcac gcccgccaac gccaaggtac gcgcgacgag ctaccatctg
     9301 ccgaacggca accaaacctt cctcaacttg gtcgagcgcg acgccaccgg ttggaaggtg
     9361 actgcgcgcg ttccagatgg tccgggcgcc acgttgccgg ccccgattct tccaccaccc
     9421 gcgccgttgc acttcaacgt gactcccgcc ggcagcggcg gaggaattcg cctgatgccg
     9481 cccgatcacc ccggcgcgag ctctccgcct tcggctcctg ctgcgcccgg gccagcgtcg
     9541 tctcccgccg cgccaaagcc cgcgctcgtt gcgccggcgc ccgcgtcttc caagtagtgg
     9601 ctagaacgcg atcgggctcg gcgttctaga gctcgccgag ccagcgatcg atgtcggacg
     9661 gggacacgga acccgtcgag acgtgtcgca cggttccgtc tttcccgatc agcacgaagg
     9721 tgggcaagac ctcgattccg tagttctttg cgacgcgacc gtcgtcgagc gctacgtcat
     9781 aggggatgcg ccagtcggtc ttcactctcg cggcgtcttc cgacgatgag tcgacgctga
     9841 tgccgacgaa ctcgacgccc ttttggtggt gccgctcgaa gctttcgacg accgccggcg
     9901 ccgaacgacg acaagcgccg caccagctcg cgaatacttc catcaacacg ggcttgccgg
     9961 cgtgatcttg caagcgaaag tgctcgccgg ggcgatcgac gatcgggagc tcgaaggcac
    10021 tcgctcgcgt tccctcaccg ggccccgaga acttgcgcgc gacgaacttc agcgcgacca
    10081 ccgcgagcgc ggcatagaga gcgtaatcga tgaggcgcga cagccagggt ttcacggcac
    10141 gagcaaccgc cgccccgccg cccatccaat gccggcgaag gcgagtgccc cgaggccatg
    10201 ggaaaagcag gcgtgccatc cctcttggct cgggcaggca acccctatcg tcaagccgcc
    10261 cgccaacccg ccgacgagac ccacgagcgc tcccgtgagc cccggcgtga gcggatccgt
    10321 cccgcgccac aagagcaaca cgccgccgga aatgaccgcg ctgaacgcga gcccagccgc
    10381 cgtgcagcga atggcatggg ccgcacggtc accgtgggaa aactccatga acgaaaccca
    10441 ctcgggcgcg gcgtgcgcga cgtaacccaa aaagatgacc ggcgtgagca ccgcgagcac
    10501 caggcggacc cgggccgaga agcgtttgcc cggcgggcga gcgaggccga cccacagaat
    10561 catcgcttgc acgatgcccc acccggctgc gccgatcagc gcggcgcgga aggtgccgtc
    10621 gatgcgcgga ttcgcccggg tgagccatgc caacaacgcg aagacgccca tcgacagcgc
    10681 gagtgagccg agcaagcggt ggctcgctgg aacacaacgc ttgtgcgaga gccctcgagt
    10741 gcaaccttca cggattgctc gcgagcactc gaggctcggc gcggccggct tgggccacgg
    10801 caagtccggc ggatcggtgt tggaggtcgt catacggttc ggttgaagcg ctcgagctcc
    10861 ttgcggagct gggtgtaccc gcgatgaact ctaagcttga ccgcagtttc ggtggtgcca
    10921 agcacgtcgg ctgcctcggc gatgctgagc cctgaaattt tggttagctg aatcgcttcg
    10981 cgatacccct cgggtagttc atcgagcgcg cgctcgagcg ccgcggacaa gtcagcaggg
    11041 agcgcggcgt cttcgggtct cggttccgga aggttgccgt cgaaactgag atcttcagtg
    11101 cgggtgctcg cgtgtcgccg ttcgtccaaa aaactgcgtc gcgcaatcgc gagcatccat
    11161 ggcaagactg gagctccgcg caggtagctt ccgcgcgcac ggtgtacctt ggcgaacgta
    11221 acctgaagca aatcttcagc gcgctcgcgc tgccgtgtaa ggcggagtag gtacccgaac
    11281 aaattcgggg ccagccggcg gtacaactcg tcaaacgcgt cggcgtcgcc gtccacgtaa
    11341 gcctccatca actcctcggg gcgggccgaa ggaagatgaa gttgtcgccc gagaaccacg
    11401 ccgggcattc ctatcgcggg caaagcgctg acgctaccga gagtcacagt ccttgggcca
    11461 cttacgattc ggccggccca ggagtttcgt cctaaatggt cgagcgcgcg ttttccgggg
    11521 ctttgggccc gaggtttcct ccaagaagcc tggaacgggc ccccctgccg aacggcctct
    11581 caggggggcc gccggctaga tgaggctctt caggtcgagc atctcctcga cgtcgggcat
    11641 aagtaccaac tcgacgcggc ggttctgctg acgtccgtca tccgtgtcgt tcttcatgac
    11701 cggatccgtc tcgccgtagc cggccgcgtg caggcgcagc gggtcgagcc cgccgccagc
    11761 ggttttaccc ccgagcggcg cgatcaagta gatgagaact tcacgcgcgc gcatcacgct
    11821 gagaccccag ttgtctccga agcgcccccc gacgagcggc ttgttgtcgg tgtgtccggc
    11881 gacttggaag tagcgtccgg cgagctgctt gtcgttccga attacgtcgg cgacggcgtc
    11941 gagg
//
LOCUS       DASTKS010001006         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1052313, whole genome shotgun sequence.
ACCESSION   DASTKS010001006 DASTKS010000000
VERSION     DASTKS010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052313"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFQ35_21300"
     CDS             <1..878
                     /locus_tag="VFQ35_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9933261.1"
                     /translation="RYEPGVQRMYDELAEHYGTVILPARPGHARDKAKVEVGVQIAER
                     WILARLRHQTFFSIEALNERIAELVEDLNGRMMRVYGKSRRELFEQIERAELKPLPAA
                     RFVCGEWKLGATVNIDYHVEYEHHYYSVPYALVREKVDVRATASTVEIFRHNERVTSH
                     PRSHQRAKHTTRPEHMPVAHQKHLEWSPTRIIGWASTVGPETAKLAETILAERRHPEQ
                     GYRSCLGILRLGKKYGHERLEAACARALSAGARSYRHVESILKHGLDRVARAEEKPAS
                     VGIFHENIRGRNYYH"
     gene            897..1637
                     /gene="istB"
                     /locus_tag="VFQ35_21305"
     CDS             897..1637
                     /gene="istB"
                     /locus_tag="VFQ35_21305"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9933262.1"
                     /translation="MLNEPTIEKLKALRLDGMCSAWQEQQTQPDNNALGFDERFGMLV
                     DAEWSFRENKRMKRSLQEAKLRVTQACIEGIDYPARRELDKAVIRQLASCRWVQEHQV
                     VIVTGATGTGKSYIACALAHQACRKGFRALYRRAPRFFDELKLAHADGSYPRLLARFA
                     RFDVIVLDDFAIAPVSDQERRDLLEVLEDRQETRATIITSQLDPKHWHDYLSDPTLAD
                     AICDRVLSRAHRLVLKGPSRRNPEKTNP"
BASE COUNT          435 a          639 c          686 g          344 t
ORIGIN      
        1 gtcgttacga acccggtgtg cagcgcatgt acgacgaact cgcggaacac tacggcacgg
       61 tgatcttgcc ggcgcgcccc ggtcatgcgc gcgacaaagc caaagtggag gtcggcgtgc
      121 agatcgccga gcggtggatc ctggcgcggc tgcgccacca gaccttcttt tcgatcgagg
      181 cgctgaacga gcggattgcc gagttggtcg aagacctcaa cggccgcatg atgcgcgtat
      241 acggcaagag ccgccgcgag ttgtttgaac agatcgagcg ggccgagctc aagccgctgc
      301 ctgccgctcg tttcgtttgt ggagagtgga agctcggggc gacggtgaac atcgactacc
      361 acgtcgagta cgagcaccat tactactcgg tgccgtacgc gctggtgcgc gagaaggtag
      421 acgtgcgcgc gaccgcgagc acggtcgaga tcttccgtca caacgagcga gtcacctcgc
      481 acccgcgcag ccaccagcgc gccaagcaca cgacgagacc cgagcacatg ccggtcgctc
      541 accaaaaaca cttggagtgg tcgccgacgc ggatcatcgg atgggcgagc acggtcgggc
      601 ccgagacggc gaagctcgcg gaaacgatcc tcgccgagcg ccgtcacccc gagcaagggt
      661 atcgctcgtg tctggggatc ctgcggctcg gaaagaagta cggtcatgag cgtctcgagg
      721 cggcgtgcgc gcgagctctt tcggcggggg cgcgctcgta ccggcatgtc gaatcgatct
      781 tgaagcacgg gctcgatcgt gtggcgcggg ccgaggagaa acccgcctct gttggcatct
      841 ttcacgagaa catccgtggt cgcaattact atcattaaca acaaggagag agagtgatgt
      901 tgaatgaacc tacgatcgaa aaactcaaag cgctccggct cgacggcatg tgctcggcct
      961 ggcaggagca acagacccaa cccgacaaca acgcgctcgg cttcgatgag cgcttcggaa
     1021 tgctcgtcga cgccgaatgg tcctttcgcg aaaacaagcg catgaagcga tcgcttcaag
     1081 aggcgaagct gcgcgttact caggcatgca tcgaaggcat cgactatccc gcgcgccgcg
     1141 agctcgacaa ggccgtcatt cgacagctcg cctcctgtcg ctgggtgcag gagcatcagg
     1201 tggtgatcgt caccggcgca acgggaaccg gcaaaagcta catcgcctgc gcgctcgctc
     1261 atcaagcgtg ccgcaaggga tttcgcgcgc tgtatcgtcg cgccccgcga ttcttcgacg
     1321 agctgaaact ggcgcatgcc gatggctcgt atccgcggct gctcgcgcgt ttcgcacgat
     1381 tcgatgtgat cgtcctcgac gattttgcga ttgcgcccgt ttccgatcag gagcggcgcg
     1441 acctgctcga ggtactcgag gatcgacaag agactcgagc gaccatcatc accagccagc
     1501 tcgatccgaa acactggcac gactacctga gcgaccccac cctcgccgac gccatctgcg
     1561 accgcgtcct gagccgcgcc caccgcctcg tgctaaaggg cccctccagg aggaaccccg
     1621 agaagaccaa cccatgacct tcgacctgag cgtcgcttcg ctccgatcgt gatgaccgaa
     1681 acgggtgatc gcgatgcggc gaaacaggtg atcgccatgg ccgaaatgcg cagctccgag
     1741 aaggcagcgt ctgttatgag cacgccggcc tcaatgtttc gcgtgtgtcc acgagccgtg
     1801 aagttcgtac ccgtccggcc aaccccgtcg tgacgcgcga gggtgtgcag gcgcagagtc
     1861 gcgcccatgg caaagcaagt ccggtggcga gttgggcgag aggaatgggc gaagcgagtc
     1921 gagcggtggc gtgagagcgg gctgacggcc gggagattcg ccgccgagca ggggatcaac
     1981 gcacgcacgc tgacgtactg gaaatggatg ctgggcaagg agtcgcgcgg ggagcggcgg
     2041 aaatggccaa gtcggaagcc gaagggagat cgcgtgttag acgggggtct ggttgagatc
     2101 acat
//
LOCUS       DASTKS010001007         6806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1722767, whole genome shotgun sequence.
ACCESSION   DASTKS010001007 DASTKS010000000
VERSION     DASTKS010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722767"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFQ35_21310"
     CDS             complement(<1..463)
                     /locus_tag="VFQ35_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9933263.1"
                     /translation="MGFSIEFAEESRVYEARIKVIGVGGSGGNAINTMIHFGLEGVEF
                     ITVNTDAQALGSNAAVEKIAIGASVTRGLGAGADPEKGRKAALEDGNRLKDIVQGADM
                     VFVTAGMGGGTGTGAAPVIAQLAREAGALTVGVVTKPFAFEGKQRMRRAEAG"
     gene            complement(624..1889)
                     /gene="ftsA"
                     /locus_tag="VFQ35_21315"
     CDS             complement(624..1889)
                     /gene="ftsA"
                     /locus_tag="VFQ35_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9933264.1"
                     /translation="MRTPQSGSEIVVGLDIGTTKVSAVVGEVDSDGITILGVGNVPCR
                     GLRKGVVSNIEWTVRSIREAIDAAQTMAGVEIKTVYAGVAGSHIRAHASDGVCAIAGR
                     EVTRADLDRVLEGARAIPVDADRMILHALPREYVVDNQDGIRDPIGMSGVRLQARVNL
                     VTAATSCVQNVVRCVERCGLTVADVVLEPLASADAVLSEDEKEIGVAVIDIGGGTTDL
                     LLYADGGIAHTSVIPAGGNNITSDVAAGLRTPMAEAERLKRNCGCALGRMISDDEEIE
                     VPGVGGHAPRRVARRLLSDIIEPRVEEIFAEARRRIEETGLLEQVSSGCVLTGGAALM
                     EGMVECAEEILGMPVRLGFPVGVKGIVQLVQGPQYATGVGLVRYGAHQLLDASQRHET
                     RSEPLRVSDDTEASPAQKHGFWSWLRAAF"
     gene            complement(2014..2886)
                     /locus_tag="VFQ35_21320"
     CDS             complement(2014..2886)
                     /locus_tag="VFQ35_21320"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9933265.1"
                     /translation="MRFAGDRMHGGSVSAPEEVYAPSESPAPAPVAEKRPNSVGGRLW
                     LMARVALGVAAVVGTSLAVAASAHRYALTSPRFSVKNLIVSGSTRFHEAEVREIAGIQ
                     AGSNLFSLDPRVVEQRLLQNPWIAQARVNRKLPSTLEVELKQRDATAIAAVGERLYLA
                     SKEGEPFKQVEGSDPIDLPVVTGLHAENLQRDRSRELDRLGVALEVLRQYARLGMSRV
                     YAAQEVHVGDAGQITLTVGKEGVVLALSTKGLRQRLLMAERVIQETRQGGRLPGVVFA
                     DNQAHPERVVVRMR"
     gene            complement(2883..4289)
                     /gene="murC"
                     /locus_tag="VFQ35_21325"
     CDS             complement(2883..4289)
                     /gene="murC"
                     /locus_tag="VFQ35_21325"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET9933266.1"
                     /translation="MFRGRVRHVHFIGVGGIGMSGLAEILRTLEFEVSGSDVRPGENT
                     RRLERLGVRIDIGHRAENVQSTDVVVYSSAIDPQNPEVKRAKELGIPVIPRAEMLAEL
                     MRVKYGIGIAGSHGKTTTTSLVATVLRAAGFDPTVVVGGRMAALGSNARLGAGDLLVA
                     EADESDGSFLRLQPTIAVVTNIDPEHLDFYKTHEALKEAFVSYVERTPFYGLSVLCLD
                     HPHVQDILPRIGRRHVTYGLSPQATYSARNIRFHGLTTSFLAFRRGEALGEFTVRMPG
                     QHNVLNTLAAVAVADELEVPLDVSKEALSTFHGVARRFTIVAEAEGVTLVDDYGHHPA
                     EVEATLSAARNAYTQRIIVAFQPHRYTRTAHLFDDFTRAFNQADVLFLTEIYAAGEKP
                     IPGISSESLAAGIAQHGHHAVQYISDKEALAEELARVAKPGDVVIALGAGDINRILPT
                     VASRLTALARGEGAGERS"
     gene            complement(4289..5443)
                     /gene="murG"
                     /locus_tag="VFQ35_21330"
     CDS             complement(4289..5443)
                     /gene="murG"
                     /locus_tag="VFQ35_21330"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9933267.1"
                     /translation="MSQTLVMAGGGTGGHVFPLLAVADVLRALAPSLRLVFVGTERGL
                     ERTLVPERGYELETLQVHPIRGGGFAGAANGVKSAALAVFKARSLLGRLKPRAVFSVG
                     GYAAGPVALAAFTARIPLALLEPNADIGLANRWVAPLVSRAYVGFPEARRFFRESTVV
                     ETGVPLRAGFTSLEPRPNDGTVRILVLGGSQGAKTLNESVPRALARLNGNVRVVHQCG
                     RAHLPETEKLYAELSMHDRAVVTPFIDDVPRALADADLVIGRAGASAVSEICLVGRPS
                     VLVPYPFAGDHQRFNALSLEQRGAARCVLAGEATVERLERELVGLLESPERLREMGER
                     ARALGKPHAAESIARDLLELAGLGVEVGKASRPGEDAAGERGRLTYSEVV"
     gene            complement(5440..6699)
                     /gene="ftsW"
                     /locus_tag="VFQ35_21335"
     CDS             complement(5440..6699)
                     /gene="ftsW"
                     /locus_tag="VFQ35_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9933268.1"
                     /translation="MSWVGAAPETTSRRGPVDVVLAAIVIALLGFGVVMVYSASVIEA
                     TVVFRDPQYFLKRQVIYALGGLSVMLFVSRFDYHRLRKFTYPLLAAVTFMMVLSVIGF
                     GHSGGGAARWLALGPIHIQPSEAAKLALVLWLAYSLEKKREKVKSFSIGMLPHFLMAG
                     FLMLLCLKQPDFGGATVLLLLTFTLLFVAGARLGYLLGAAMLGVLFAAWAVRFTPYRW
                     ERMLAWFNMADHRQDLAYQPFQSVMSFGSGQTTGMGLGRGLQVLYLPEAHTDFISAIV
                     GEELGFLGILLLCGAYLVVVVRGVKAALNAEDQYGAYIAFGISVLFGVQALVNMAVAM
                     SMLPTKGLTLPFVSYGGSSLLVCAASMGILLSVSRSAGASGNVRVARPKDAKGGLAEA
                     SALLVSEAGFAPEAVGLRRRAADKVRA"
BASE COUNT         1179 a         2341 c         2180 g         1106 t
ORIGIN      
        1 ggcccgcctc cgcgcgccgc atgcgttgct tgccttcgaa ggcgaacggc ttcgtcacca
       61 cgccgacggt cagcgcgcct gcttcacgcg cgagctgggc gatgaccggg gcggctccgg
      121 taccggtgcc accgcccata ccggccgtga cgaacaccat gtcggcgccc tgaacgatgt
      181 ctttgagtcg attgccgtcc tcgagcgcgg ctttgcggcc cttttccggg tccgcgccag
      241 cgccgaggcc gcgcgtcacg ctcgcgccga ttgcgatctt ttcgaccgcg gcgttcgagc
      301 cgagcgcctg ggcatcggtg ttcaccgtga tgaactccac gccctccagg ccaaagtgga
      361 tcatcgtgtt gatggcgttg ccacccgagc cgccgacgcc gatgaccttg atgcgagcct
      421 cgtagacgcg agactcctcc gcgaattcga ttgagaaacc catttgttgt cctcccggtt
      481 cggctgttcg ccgaagccac ccgttcgcgc cctcgaggtt gcctctcctc tttgtgggtg
      541 cgaccaggct gcccggttgc tttctgctct gtgaatgccc tggggtacgt ttttaagcta
      601 agtgacgaag cgagccgcga gcgtcagaac gccgcgcgca accagctcca aaatccatgt
      661 ttttgcgcgg gcgacgcttc cgtgtcgtcg ctcacccgca gcggttcgct gcgcgtctcg
      721 tgtcgctgag aggcgtccaa cagctggtgg gcgccgtaac gcacgagccc gactcccgtc
      781 gcgtactgag gtccctgcac cagctgcacg atgcccttca cgccgaccgg gaagccgagg
      841 cgcaccggca taccgaggat ctcctcggca cactcgacca tgccctccat caacgccgct
      901 ccaccggtga gcacgcagcc agagctcacc tgctcgagca ggccagtctc ttcgatgcgg
      961 cggcgcgctt cggcgaagat ctcttcgacg cgcggctcga tgatgtcgct caagaggcga
     1021 cgcgcgacgc gacgcggcgc atgcccgccc acgcccggca cctcgatctc ctcgtcgtcg
     1081 gagatcatcc gcccgagggc gcacccgcag tttctcttca ggcgctccgc ctcggccatc
     1141 ggggtccgga gccccgccgc cacgtcgctc gtgatgttgt tgccaccagc cgggatgacg
     1201 ctcgtatggg cgatccctcc gtcggcgtag aggagcaggt cggtcgtgcc gccaccgatg
     1261 tcgatgaccg cgacgccgat ctctttttcg tcctcgctca gcaccgcgtc cgcgctggcg
     1321 agcggctcga ggacgacgtc ggccaccgtg agaccgcagc gctcgacgca gcgcaccacg
     1381 ttttgcacgc acgacgttgc cgccgtaacg aggttcactc gggcctggag ccggacgccg
     1441 ctcatcccga tcgggtcgcg gatgccgtcc tggttgtcga cgacgtactc gcgcggtagc
     1501 gcgtggagga tcatccgatc cgcgtcgacg gggatggcgc gcgcgccttc gaggacgcga
     1561 tcgagatccg cgcgggtcac ttcgcgacca gcgatggcgc aaacgccatc cgaggcgtgc
     1621 gcgcgaatgt gacttccggc aacgccggca tacaccgtct tgatctcgac gcccgccatg
     1681 gtctgtgcgg cgtcgatggc ttcacggatc gagcgaacgg tccactcgat gttgctcacg
     1741 acccctttgc gcagcccgcg gcaagggacg ttgccaacgc ccaagatggt gatgccgtcc
     1801 gagtcgactt cgccgaccac cgccgaaact ttggtggtcc cgatgtcgag tccgacgacg
     1861 atctcgcttc ctgattgggg ggttctcatg tgtccgtgga tccgaggccg acaaagacct
     1921 cgaaaaggcc gctcgaggct gcgccgacgc tgccatggtg ggccgaggct ggccaagttt
     1981 tgaccggcga gaaatcgcga ttcgatgccg ggctcatcgc atcctcacga cgacacgctc
     2041 ggggtgagcc tgattgtcgg cgaaaacgac gcccggaagc cgcccgccct ggcgcgtctc
     2101 ttggatgaca cgctccgcca tgaggagccg ctggcgcaac cccttggtgc tgagggccaa
     2161 gacgacaccc tctttgccga cggtgagcgt gatctggccg gcgtcaccca cgtgcacttc
     2221 ttgcgccgcg tagactcgac tcatcccgag gcgcgcgtat tgacgcagga cctcgagcgc
     2281 caccccgagc cgatcgagct cgcgagagcg atcgcgctgc aggttctccg cgtgcagccc
     2341 ggtcacgacc ggcaggtcga tcgggtcgct tccctccacc tgcttgaatg gctctccctc
     2401 tttactcgcc aagtagagcc gctcaccaac cgccgcgatc gcggttgcat cgcgttgctt
     2461 cagctcgact tcgagcgtcg agggaagctt tcgattcacg cgggcctgcg cgatccacgg
     2521 gttttgcagc agccgctgct ccaccacgcg cggatccagt gaaaataggt tgctccccgc
     2581 ttggatgccg gcaatctcac gcacctccgc ttcgtggaag cgcgtcgagc ccgacacgat
     2641 caggttcttc accgagaacc taggggaagt gagggcgtag cgatgcgcgc tcgcagccac
     2701 cgcgagagaa gtgccgacga cggccgccac gccgagcgcg acgcgagcca tgagccaaag
     2761 gcggcctccg acgctgttcg ggcgcttttc cgccaccggg gccggcgccg gactctcgct
     2821 cggtgcgtac acctcttccg gagcgctcac gctaccgccg tgcatgcgat cgcctgcgaa
     2881 cctcaagacc gctcccctgc cccttctccc cgggcaagcg cggtgaggcg cgaagcgacg
     2941 gtcggaagaa tccggttgat gtcgcccgcg ccgagcgcaa tcacgacgtc gccgggcttg
     3001 gcgacacgcg caagctcctc ggccagcgcc tccttatcgg agatgtactg gaccgcgtga
     3061 tggccgtgct gcgcgatccc ggccgcgaga ctctcgctgc tgatccccgg aatcggcttc
     3121 tcgccggccg cgtagatctc ggtgagaaag agcacgtccg cttggttgaa ggcgcgcgtg
     3181 aaatcgtcga agaggtgcgc ggtgcgggtg tagcggtggg gctgaaacgc cacgatgatg
     3241 cgttgggtgt aggcgttgcg ggccgcgctg agcgttgctt cgacctcggc cgggtggtga
     3301 ccatagtcgt ccaccaaggt gacgccctcg gcctccgcca cgatcgtgaa gcggcgcgcg
     3361 acgccgtgga aggtcgaaag cgcctccttc gagacgtcga gcggcacttc gagctcgtcg
     3421 gcgacggcca ccgccgcgag tgtgttgagc acgttgtgct gacctggcat gcgcacggtg
     3481 aattcaccca gcgcttcccc acggcgaaag gcaagaaaac tggtggtcag cccgtgaaag
     3541 cggatgttgc gcgccgagta cgtcgcctgc ggcgaaagcc cgtaggtcac gtggcgccga
     3601 ccaatgcgcg gcaggatgtc ctgcacgtgc gggtgatcca gacacaagac cgagagcccg
     3661 taaaaaggcg tgcgctctac gtaagatacg aacgcttctt tgagcgcttc atgagtcttg
     3721 tagaagtcga ggtgctcggg gtcgatgttg gtgacgaccg cgatggtggg ctggagccgc
     3781 agaaaggagc cgtcgctctc gtcggcttcg gcgacgagca gatcccccgc gcccaggcga
     3841 gcgttgctgc cgagcgccgc catgcgcccg ccgacgacca cggtggggtc gaagccggcc
     3901 gcgcgcaaca cggtggcaac cagtgacgtc gtcgtggttt tcccgtgcga gccggcgatg
     3961 ccgatgccgt acttcacgcg catcagctcc gcgagcatct cggcgcgcgg gatgacgggg
     4021 attccgagct ctttggcgcg cttgacctcg gggttttgcg gatcgatcgc gctcgaataa
     4081 acgacgacgt ccgtcgactg cacgttctcc gcgcggtgac cgatgtcgat gcgcacgccg
     4141 agccgctcga ggcgccgcgt gttttcgccg ggccgcacgt ccgaaccaga gacctcgaac
     4201 tccagggtgc gaaggatctc tgcgaggccg ctcatgccga tgccgccgac gccaatgaag
     4261 tgcacgtgtc gcacgcgacc gcgaaacatc agactacctc cgaatacgtg agccgcccgc
     4321 gctccccggc cgcatcctcg cccggtcgcg atgccttgcc gacctcgact ccgaggccag
     4381 cgagctcgag caagtctcgc gcgatggact cggccgcgtg gggcttgccg agcgcccgcg
     4441 cccgctcgcc catctcgcgc agccgttcgg gagactccaa gagcccgacg agctcccgct
     4501 cgagtcgctc gaccgtcgct tcgcccgcga gcacgcagcg ggccgccccg cgctgttcga
     4561 gcgaaagcgc gttgaaccgc tggtgatcac ccgcaaacgg atacggaacc agaacgcttg
     4621 gtcgcccgac taggcaaatc tcgctgacgg cgctcgcgcc ggctcgaccg atcaccaggt
     4681 cggcgtcggc caacgctcgc ggcacgtcgt cgatgaacgg agtgacgacg gcgcggtcgt
     4741 gcatcgaaag ctccgcgtaa agcttctcgg tttcgggcaa gtgcgcccga ccgcactgat
     4801 gcacgactcg gacgttgccg ttcaagcgag cgagcgcgcg aggcaccgac tcgttcagcg
     4861 tcttcgcccc ttggctcccg ccgagcacga ggatgcgcac ggttccgtcg ttcggacgcg
     4921 gctcgaggga tgtgaaccca gcgcggagcg gaacccccgt ctcgacgacg gtgctctcgc
     4981 gaaagaatcg acgcgcttcg gggaagccca cgtaggcgcg gctcactagc ggtgccaccc
     5041 agcggttggc aagacctata tcggcattcg gctcgagcag cgcgagcggg atgcgcgccg
     5101 tgaacgcggc gagcgcgacc gggcctgcgg cgtagccacc cacggagaat acggctcgcg
     5161 gcttcaaccg gccgagcagt gagcgcgcct tgaagaccgc gagcgcggcg ctcttcactc
     5221 cattcgcagc gcccgcgaag ccgccgcctc ggatggggtg cacctgcagg gtctcgagct
     5281 cgtagcctcg ctccggcacc aacgttcgct cgaggccgcg ctccgttccg acgaacacga
     5341 gccgaagcga aggagccaac gcgcggagca cgtccgcgac ggcaagcaac ggaaaaacgt
     5401 gtccgcccgt gcctccgccg gccatcacca gggtctggct catgcccgca ccttgtcggc
     5461 agcacggcgc cgcaaaccta cggcttcggg agcaaagcca gcttcgctga cgagcagcgc
     5521 actcgcctcg gcgaggcccc ccttggcgtc tttcgggcgc gccacccgca cgttgccgct
     5581 cgcgccggcg ctgcgcgaca cgctgagcag aatgcccatc gacgccgcgc agacgaggag
     5641 cgaagagccg ccgtagctga cgaacggcag cgtgagtccc ttcgtcggga gcatcgacat
     5701 ggcgaccgcc atgttcacga gcgcttgcac gccgaacagc accgaaatgc cgaaagcgat
     5761 gtacgcgccg tactgatctt cggcgttgag cgccgctttc accccgcgca cgacgacgac
     5821 cagatacgcg ccacagagca gcaaaatgcc gagaaagccg agttcttcac cgacgatggc
     5881 ggagatgaaa tccgtgtgcg cttcgggcag atagaggacc tgcaagccgc ggccgagccc
     5941 catgccggtc gtttgcccgg aaccgaagct catcaccgat tgaaacggct gataagcgag
     6001 atcctgacga tggtcggcca tgttgaacca cgccagcatg cgttcccagc gataaggggt
     6061 gaagcgcacc gcccacgccg cgaacaggac gcccagcatc gccgcaccga gcaggtagcc
     6121 gagccgcgcc cctgcgacga acaagagcgt gaaggtcaaa agcaagagca ccgtcgcgcc
     6181 gccgaagtcg ggctgcttga ggcagagcag catcaagaag cctgccatca agaagtgcgg
     6241 cagcatgccg atcgagaagc tcttcacctt ttcgcgcttc ttctcgagcg aataagcgag
     6301 ccacagaacc agcgcgagct tcgcggcctc ggagggctgg atgtgaatcg gcccgagcgc
     6361 gagccagcgc gccgctccac cgcccgagtg cccgaagcca atcacgctga gcaccatcat
     6421 gaaggtgacg gcggcgagca gcggataggt gaattttcgc agccgatgat aatcgaagcg
     6481 cgagacgaac agcatcacgg agaggccgcc gagcgcgtaa atcacctggc gcttcaaaaa
     6541 gtactgcggg tcacggaaca cgaccgtcgc ctcgatgacg ctcgcgctgt agaccatgac
     6601 gaccccgaac ccgagcagcg cgattacgat cgctgccaga acgacgtcga ccggtccgcg
     6661 acgcgaggtc gtttccggtg ctgcgcccac ccagctcatg acttcacctc gttctgcgcg
     6721 gccgcgaggc gcgccaccgc ttctacgaat ttgtctccgc gatcggcgta gctcttgaac
     6781 atgtcgaagc tcgagcacgc cgggct
//
LOCUS       DASTKS010001008         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1052644, whole genome shotgun sequence.
ACCESSION   DASTKS010001008 DASTKS010000000
VERSION     DASTKS010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052644"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2447)
                     /gene="lon"
                     /locus_tag="VFQ35_21340"
     CDS             complement(<1..2447)
                     /gene="lon"
                     /locus_tag="VFQ35_21340"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9933269.1"
                     /translation="MSERRQLPLLALRGAVAFPGTTVPIAVGRAASLKAVEEALKSDR
                     TVFAVAEREGIDANSPDRLHNMGVIAHIGEVQRGLGGLQLLLTPQERAAVLEYRTQGG
                     VPRAVVRPVAEMAPLNENDPAFLALSREVRERAIEFGKQRGLSEEVLVQVIDAVNGPG
                     EFADLVATHLELPVAEKQALLETLGVEERLRKVLVHLQRRIEQLSAQQRIRESVTEEI
                     GAKQREMFLREQLKAIHKELGDSDDQANEIAVLREKLEKLELPEHAAKEAQRELGRLE
                     RVGAESMEAQVIRNHLQWMTELPWNTRSEEKLDLKAAEQVLDDDHYGLSDVKDRVLEF
                     LSVRELQLRQSSGATDSDDDKDKRRGPKGSILLFVGPPGVGKTSIAKSIARSLGRKYV
                     RIALGGVHDEADIRGHRRTYVGAMPGRVIAGLKQAGTKNPVFLLDEVDKLGTSFHGDP
                     ASALLEVLDPAQNSEFTDHYLNVAFDLSEVLFIATANVLHTIPPALLDRMEVVNFAGY
                     TEKEKLEIAKRYIVPRQLEESGLAALDPRPSLDESALHTVISRYTRESGVRQLERQIG
                     SVARKLARKVASGESFEPRLGPEHVQKLLGRPKVHPERASEVDEIGAATGMYYTPAGG
                     DIMFVEAAVRRLHTRPRPENSVEADGWGDVALILTGQLGDVMKESARAALTYAATHAR
                     ELQIPQQSLGSIEAHIHVPAGAIPKDGPSAGVTIATALVSAMTGRPVRKDVSMTGEIT
                     LRGRVLPIGGVKEKVLGAHRAGIRQIILPKQNEADLDDVPAEVQRELEFHFAETIDDV
                     LRVAIADTAKANTAPAR"
     gene            2581..>3303
                     /locus_tag="VFQ35_21345"
     CDS             2581..>3303
                     /locus_tag="VFQ35_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933270.1"
                     /translation="MQTSAQAHLAVLASAVSSVELGPVGVAAQVTDGFRAYQALTADR
                     DALRGLEPELVRLTREGSPAAIIYAALLLRGLGRDVVPLLASYREDHRPCSVFPGGCQ
                     GVTHWLNEAARWVTTGEFWSDPDRLLGYDIESLARANWFELPSARLLEAARAGRRRDG
                     RMASGNWVFTFTELFVDASKLRRARPLLENLLTNGQPPVRLYAALLLREADRATGERA
                     LASLAGAGGEVARLVPSFWGGRS"
BASE COUNT          507 a         1069 c         1085 g          642 t
ORIGIN      
        1 ttacgcgcag gggccgtgtt cgccttcgcg gtgtcggcga tcgcgacgcg caggacgtcg
       61 tcgatcgttt cggcgaagtg gaactcgagc tctcgttgca cctctgccgg cacgtcatcg
      121 agatcggctt cgttctgctt aggcagaatg atttgccgaa tgccggcgcg gtgcgcgcca
      181 agcactttct ctttgacgcc gccgatcggg agcacccgac ctcgcagcgt gatctcgccc
      241 gtcatcgaga cgtctttgcg cacgggtcgg cccgtcatcg ccgaaaccaa cgcggtcgcg
      301 atcgtgacgc cggcgctggg cccgtccttc ggaatggcgc ccgccgggac gtggatgtgc
      361 gcctcgatcg agccgaggct ctgctgggga atttggagct cacgggcgtg cgtcgcggcg
      421 taggtcagcg ccgcgcgtgc gctctccttc atcacgtcgc cgagctgacc ggtgaggatc
      481 agggcgacgt caccccagcc gtcggcctcg acgctgttct cggggcgagg tcgagtgtgc
      541 agccggcgta cggcggcttc cacgaacatg atgtcaccgc ctgccggtgt gtaatacatg
      601 cctgttgccg cgccgatttc gtcgacttca ctcgcgcgct cggggtgcac tttggggcgc
      661 cccaagagct tctgcacatg ctcggggccg agacgcggct cgaaagactc acccgaggcg
      721 acttttcgcg cgagcttcct cgcgaccgag ccgatttgtc gctcgagttg gcgcacgccg
      781 ctttcgcgcg tgtaccgcga tatcaccgtg tgcaacgccg actcgtccaa cgacggcctc
      841 gggtcgagcg cggcgagccc cgactcttcg agctgacgcg gaacgatgta gcgcttggcg
      901 atttcgagct tttccttctc ggtgtacccg gcgaagttca cgacctccat gcgatcgagg
      961 agcgcgggcg gaatcgtgtg cagcacgttc gcggtcgcga tgaacaagac ttcgctgaga
     1021 tcgaacgcca cgttcaggta gtgatccgta aactccgagt tttgcgccgg atccaacacc
     1081 tcgagcaggg cgctcgccgg gtcgccgtgg aacgacgtcc cgagcttgtc gacctcatcg
     1141 agcaagaaca ccgggttctt cgtgcccgcc tgcttcaagc cggcgatgac gcgcccaggc
     1201 attgcgccca cgtaggtgcg gcggtgcccg cgaatatcgg cctcgtcgtg cacgccgccg
     1261 agggcgatgc gcacgtactt gcgcccgagg gaccttgcga tgctcttcgc gatgctggtc
     1321 ttgcctacgc caggaggccc gacgaagagc aagatcgaac ccttggggcc ccggcgcttg
     1381 tctttgtcgt catcggagtc cgtcgccccg gagctctgac gaagttgcag ctcacgcacg
     1441 ctcaagaact cgagcacgcg atccttcacg tccgagagcc catagtgatc gtcgtcgagc
     1501 acctgctcgg cggctttcag gtcgagcttc tcctccgagc gggtgttcca agggagctcc
     1561 gtcatccact gcaggtggtt gcggatgact tgcgcctcca tcgactcggc gccgactcgt
     1621 tcgagccgcc cgagctcgcg ttgtgcctcc ttggcggcgt gctccgggag ctcgagcttc
     1681 tcgagtttct cccgcaaaac agcgatctcg ttggcctggt cgtcgctgtc cccgagctcc
     1741 ttgtgaatgg ccttgagttg ctcacggagg aacatctccc gctgcttggc gccgatttcc
     1801 tcggtcacgc tctcgcggat tcgttgctga gcgctcagct gctcgatacg tcgttgcagg
     1861 tggaccagta ccttgcgcag ccgctcttcg accccgagcg tctcgagcag agcctgcttc
     1921 tcggcgacgg gcagctcgag gtgggtcgcg acgagatcgg cgaattcacc ggggccgttt
     1981 acggcgtcga tgacttgcac cagcacctct tccgagagcc cgcgctgctt tccgaattcg
     2041 atcgcgcgct cccggacttc tcgcgagagg gcgaggaaag ccgggtcgtt ctcgttcaac
     2101 ggagccattt cggcgacggg acgaaccacc gctctcggta cgccgccttg cgtgcggtac
     2161 tcgagcacgg ccgctcgctc ttgaggagtg agcaggagct gaagcccccc gagccctcgc
     2221 tggacttcgc cgatatgtgc gatgacgccc atattgtgga ggcggtccgg ggagttggcg
     2281 tcgataccct cgcgctcggc aaccgcgaag acggtgcggt cggacttcag cgcctcttcg
     2341 accgctttca gcgacgccgc acgcccgacg gcgatgggaa cagtggtacc aggaaaggca
     2401 accgcaccgc gcaaggcaag tagcggcaat tgacgacgtt ctgacattcc ctttgcatgc
     2461 gcactggttc cggtcggcgc tagtcctagc cacctgctcg accgggtagg aattcgacga
     2521 aatcggttgc ctgaaaccgc gcccgcggac agcgcgtaag aatctggata agctcgctgc
     2581 gtgcaaacgt ccgcgcaggc gcaccttgca gttcttgctt cggcagtcag ctcggtcgag
     2641 ctcgggcccg tcggcgtggc ggcgcaggtt acggatggtt ttcgggctta tcaggccctc
     2701 acggccgacc gggacgcgct gagaggcctc gaacccgagc tcgttcggct aactcgagag
     2761 ggttctccgg ctgccatcat ttatgcggcg ctgctcctac gtggccttgg gcgcgatgtg
     2821 gtgccgctgc ttgccagcta ccgcgaagac cacaggccgt gcagcgtctt tccgggtggc
     2881 tgccagggcg tgacgcactg gctgaacgaa gccgcgcgtt gggtgacgac aggggaattc
     2941 tggagcgacc ccgaccggct cttggggtac gacatcgagt ccctggcccg cgccaattgg
     3001 ttcgagctgc cgagcgccag gctgctcgag gcagcgcgag cggggcgccg gcgcgatggg
     3061 cgcatggcct cggggaattg ggtcttcaca ttcaccgaac tattcgtaga cgcttcgaag
     3121 ctgcgccgag cgcggccgct gctcgagaat ctgctcacga atgggcaacc tccagttcgg
     3181 ctgtacgccg ccttgctatt gcgcgaggcg gaccgagcga cgggcgaacg cgccctcgcg
     3241 tcgttggcgg gcgctggtgg cgaagtagcg cggttggtcc cgagtttttg gggaggccgg
     3301 tcg
//
LOCUS       DASTKS010001009         5548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1947556, whole genome shotgun sequence.
ACCESSION   DASTKS010001009 DASTKS010000000
VERSION     DASTKS010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947556"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFQ35_21350"
     CDS             <1..564
                     /locus_tag="VFQ35_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933271.1"
                     /translation="SKQEDPLRIIGLLAKTFAQRLKHPLATVFHSASPYGLGDHHAVK
                     YSVEPATPQRFAALSAGSETDYLRDALAESLRDEPIELKVFIHVMSPRMVPLGYRSLA
                     HIVEDATLDWSQLGAIKVHVASIELPQQDATNADSLQTAERFRFNPWHALAAHRPLGS
                     LNRARLSAYRASQSYRSRNDVPSDASG"
     gene            649..1491
                     /locus_tag="VFQ35_21355"
     CDS             649..1491
                     /locus_tag="VFQ35_21355"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9933272.1"
                     /translation="MAHRKYLLVLGVFALLAWFTAGCQRRAEPSAANGASKGAVVAAT
                     GVAPAVPKEDSLRQVIRKAEMQLEVAAPSRALSRATEIAERHGGFVANTATNSGQDAG
                     AVVSLTLRVPAGHFTRVLEELRALGSGASMERVSSEDVSDECLDLEARIKTQRALEQQ
                     FLEILKRAEKVEDALKVERELATVRAEIERLDGRRKFLAREVALSTIDVSLTQERALV
                     SASWRSLGDAARHAYADAIDNAAGLVELALRVIGALLPIALLLGAPAAWGIRHLRRKR
                     AVAR"
     gene            complement(1526..1729)
                     /locus_tag="VFQ35_21360"
     CDS             complement(1526..1729)
                     /locus_tag="VFQ35_21360"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HET9933273.1"
                     /translation="MSSDEERMLRIETKIAYQDKLIADLNDVIVDLNRTVRDYARRLE
                     TVERAFRNELERRDMPNEKPPHY"
     gene            complement(1752..4232)
                     /locus_tag="VFQ35_21365"
     CDS             complement(1752..4232)
                     /locus_tag="VFQ35_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HET9933274.1"
                     /translation="MSALSPICPPEVLAPAGDIESLRAALRAGADAVYFGLRELNARA
                     RAKNFERGELASVVAELHRHDVKAYVALNTLVFDSELELLDESVRACVDAGVDALIMQ
                     DLGAVLRVRELAPNLAIHASTQMTCTDAGSVAFAASLGASRVVLARELSLAEIAAIRQ
                     ATPTELEVFVHGALCVAYSGQCLTSEAIGGRSANRGACAQACRLPYDLYVDGARRDLG
                     ERAYLLSPEDLEASALVPELARAGVTSLKIEGRLKGPEYVGAVTSLYRRAVDALESGQ
                     APNEDERRLALATFTRGSGPGFLRGTDHQRLVEGRSSDHRGLCCGDVVRRVERRGRAR
                     LGLRLEVELARGDGVLLEGGLAGDGELGGRVWSIEQHGREVELARAGEEIELWLGPEV
                     RIPERLTLPRRLFKTNDPRAEQEIRRRLTESPHRAPLDFTVRAQVGVALELEVNTQGG
                     RRANYRGDAALDRARGVGTSLSVIREKLERLGDTPYRLRSLRGEVDADVFVPVSLLNA
                     ARRALVESLSEAERPTPPSAQQTERREFSLGDFPEPGLFALCRNVDQARAALRAGVNG
                     VYLDFLELTGTGSALRALRTEFPSRVIGVAPPRIRKPGEEKIDRYLRELAPDAMLVRG
                     LGALGTVREVGEGIRCVGDFSLNVASRASALAVLERGVVAFTPAFDLDAAQLEALLRT
                     EVGPYAEVVIHHPMPLFHMEHCVYAALLSNGGDYKSCGRPCEAHAIELRDRSGRVHPV
                     VADVGCRNTVFHGASQSAASLAPALRAAGVRRFRVELLRESAAETEVVVGAYQRLLAG
                     ELSGREVWNQLRTEKGYGIVRGSLRVVS"
     gene            4485..>5548
                     /locus_tag="VFQ35_21370"
     CDS             4485..>5548
                     /locus_tag="VFQ35_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933275.1"
                     /translation="MQTSARFAAVIVLATGAWACSGGDGASDSGSGGNANPGGAANGG
                     ASGNGGASGFTSVGQGGKSGSGGTNAAGAATSGGTSAQGGTASSSGGRSDGTSGGSTP
                     MSGGSSSGGNPGGTSNRGGATSSGGANSGGSGGVGNTGGAAGSGGGTAGGGTSGGVSI
                     CKFASGLNIAWVNFANDVPNPNISTFNTIFKNSHDAGGRVIRWWFHTNGTKTPGYDSS
                     GMAMKLVQSHIDGVKAILSAAQQNGVRVTLSLWSFDMLQDNAMTAWTNNQKLLEVDEN
                     RQAYIDNYLIPLVNAIKGHPGLYAWEIFNEPEGMGPKGWATHRTTEAAIQKTVNWFAA
                     AIHTADPSALVTNGAQTFDY"
BASE COUNT         1066 a         1822 c         1791 g          869 t
ORIGIN      
        1 agcaaacaag aagacccgct gcggatcatc ggccttctcg ccaaaacctt cgcccaacgg
       61 ctgaagcatc cgctcgccac ggtgtttcac agcgcgtcac cgtacgggtt gggggaccat
      121 cacgcggtca aatactcggt ggagccggcg accccgcagc gctttgccgc gctcagcgcg
      181 ggaagcgaga ccgactacct ccgggacgcg ctcgccgaaa gcctgagaga cgaacccatc
      241 gagctaaaag tcttcatcca cgtgatgtca ccgaggatgg tgccgctcgg ctaccgctca
      301 ctcgcgcaca tcgtggaaga cgccactttg gactggtctc agctcggcgc aatcaaagtt
      361 cacgtcgcga gcatcgagct cccgcagcaa gacgcgacca acgcagactc tctccaaacc
      421 gcggagcgtt tccgtttcaa cccctggcat gccctcgccg cgcaccgtcc gctcgggagt
      481 ttgaaccggg ctcgcctcag cgcctaccga gcaagccaga gctatcgctc gcgaaatgac
      541 gtaccgagcg acgcgagcgg ctgactagcg ccaaacgact cggaggcgca cgaccgtaag
      601 tttccgtctg ctcgcgcgtt ggaacctcga atgcgttcac gaggtcccgt ggcccatcga
      661 aagtatctgt tagttctcgg cgtgtttgcg ttgctcgcgt ggttcactgc cggctgccag
      721 cggcgagccg agccgagcgc cgcgaacggc gcgagcaagg gcgcagtggt agccgcaact
      781 ggcgtggccc ccgccgtccc caaagaggac agcctacggc aagtgatccg caaagccgaa
      841 atgcagctcg aggtcgcagc gccgtctcga gcgctatcgc gcgcgactga aatcgcagag
      901 cggcatggtg gattcgtagc gaataccgcg acgaacagcg ggcaggacgc cggcgccgtg
      961 gtgtcgctca cgctccgcgt acctgccggt cacttcacgc gcgtcctcga agagcttcgc
     1021 gcgctcggct ctggcgcgag catggagcgc gtcagcagcg aagacgtgag cgatgaatgc
     1081 ctcgacctcg aagcgcggat aaagacgcaa cgggctctgg aacagcaatt cttggagatc
     1141 ttgaagcgtg ccgaaaaagt cgaagacgct ttgaaagtcg aacgagaact tgcaaccgtt
     1201 cgcgccgaga tcgagcgtct cgacggtcgg cgcaagttcc tcgctcgaga ggtcgcactt
     1261 tcgacaatcg atgtttcgct gacccaagag cgagcattgg tctcggcctc gtggcgcagc
     1321 ttgggcgacg cagcgcgaca cgcctacgcc gacgcaatcg acaacgccgc cggcctcgtg
     1381 gagttggctc tccgcgtcat tggcgcattg ctgccgatag cgctcctact cggtgcccca
     1441 gccgcatggg gaatcagaca tctacgccga aagcgcgccg tggctcggtg acgcgcgagc
     1501 cgcgcttgcc caagctcgag cggcgtcagt agtgaggcgg cttctcgttg ggcatgtcgc
     1561 gacgttcgag ctcgttgcga aaagcgcgct cgaccgtctc gagccggcgt gcgtaatccc
     1621 tcaccgtgcg attgaggtcg acgatcacgt cgttgagatc ggcgatcagc ttgtcttgat
     1681 aagcgatctt cgtctcgatg cggagcattc gttcttcgtc tgagctcatg gcagagatct
     1741 cttcggggtc actacgatac gacgcgcaag gatccgcgca cgatgccgta gcccttttcg
     1801 gtgcggagtt ggttccaaac ctcgcggccg ctgagttcgc cagcgagaag gcgctgatac
     1861 gcgcccacga cgacctccgt ttcggccgcg ctctcgcgga gcagctcgac gcgaaagcgc
     1921 ctcacacccg ccgcgcgcag tgcgggcgcc agcgacgcgg cgctctgcga ggccccgtga
     1981 aatacggtgt tgcggcagcc gacatcggcg accaccgggt gcactcgacc agagcggtca
     2041 cgcagctcga tcgcgtgcgc ttcacagggg cgcccgcaac tcttgtaatc tccgccgttc
     2101 gacaggagcg ccgcgtacac gcagtgctcc atgtgaaaga gcggcatggg gtggtggatg
     2161 acgacttcgg cgtatggtcc gacttcggtc cgcaacaacg cttcgagctg ggccgcgtcg
     2221 aggtcgaacg cgggcgtgaa cgccacgacc ccgcgctcta ggaccgccag cgcgctcgcg
     2281 cgagatgcga cattgagcga aaaatcgccg acacagcgga tcccctcccc gacctcgcga
     2341 acggtaccga gagcaccgag cccacggacg agcatcgcgt cgggtgcgag ctcacgcaaa
     2401 tagcggtcga tcttctcttc acctggcttg cgaatccgcg gtggggcgac tccgatgacg
     2461 cgagaaggaa actcggtgcg caaggcacga agcgcgctac cggtgccggt gagctctagg
     2521 aagtcgaggt aaacgccgtt cacgccagcg cgcagcgcgg cgcgggcctg gtcgacgttt
     2581 ctgcacagtg caaacaggcc gggctcagga aagtcgccga gagaaaactc gcggcgctcc
     2641 gtttgctgcg cgctcggagg cgtcggccgt tcggcttcgc tcaacgactc gacgagcgcc
     2701 cggcgcgccg cattgagcag agaaaccgga acgaaaacgt ccgcgtccac ttcgccacga
     2761 agcgagcgca agcgatacgg cgtgtccccc aggcgctcga gcttctcccg aatcacgctg
     2821 agcgacgtgc cgacgccgcg cgcgcggtcg agcgccgcat cgccgcgata gttcgctcgg
     2881 cgtccgcctt gggtattcac ttcgagctcg agcgccaccc cgacctgcgc tcgcaccgtg
     2941 aagtcgaggg gggcgcggtg gggtgactcg gtcagacgcc gcctgatctc ctgctcggcg
     3001 cgcggatcgt tggtcttgaa caaacgccgc ggcagtgtga ggcgctcggg gatgcgcacc
     3061 tccggaccca gccagagctc gatctcctcg cccgcccgcg cgagctcgac ctctcgcccg
     3121 tgctgctcga tcgaccaaac ccgaccgcca agctctccgt caccggcgag cccgccttcg
     3181 agcaacacgc cgtcgccgcg cgcgagctcg acttcgagcc gcaggccaag acgcgcgcga
     3241 ccgcggcgct cgacccggcg aaccacgtcg ccgcagcaga gcccacggtg atccgatgat
     3301 cgaccttcga cgagccgttg atgatccgtg ccgcgcaaaa agcctggccc cgacccgcgc
     3361 gtgaacgtcg cgagcgcgag gcgacgctcg tcctcgttcg gcgcttgccc ggactcgagc
     3421 gcgtcgaccg cacgccggta gagcgacgta accgcgccga cgtactcggg ccccttcaac
     3481 cgcccttcga tcttgagaga cgtcacgccc gcgcgcgcga gctccggtac caacgcgctc
     3541 gcttcgaggt cctccggtga caagagataa gcgcgctcac cgaggtcgcg tcgagcgcca
     3601 tcgacgtaca ggtcgtaagg cagccggcaa gcttgcgcac aagcaccgcg gttcgcgctg
     3661 cgtccgccga tcgcttcgct ggtcaggcac tgccccgaat acgctacgca gagcgcgccg
     3721 tgaacgaaca cctcgagctc ggtgggcgtc gcctggcgaa tcgccgcaat ctcggcgagg
     3781 gaaagctctc gagcgaggac cacgcggctc gctccgagtg acgccgcaaa tgcgaccgac
     3841 cccgcgtcgg tgcaagtcat ttgagtagaa gcgtgaatcg cgagattggg agccaactcc
     3901 cgcacgcgca ggacggcgcc gagatcctgc atgatgagag cgtcgacgcc cgcgtcgaca
     3961 caagcccgaa cactctcgtc gagcagctcg agctcggagt cgaacaccaa agtgttgagc
     4021 gcgacgtacg ccttcacgtc gtgtcggtgg agctccgcga ccacgctcgc gagttcgccg
     4081 cgttcgaagt ttttcgcgcg cgcccgcgcg ttcagctcgc gtaagccaaa gtacaccgcg
     4141 tccgctcctg cgcggagcgc agctcgcaac gactcgatgt cgcccgctgg tgcgagcacc
     4201 tcgggagggc agattgggga aagagcagac atctgagctt cggagcacgg agccatagct
     4261 ggcacgcgcc gaagacgctc gtttaggcca gaaaatctcg gatatttgta ggaatgaagg
     4321 tcgagcggag ctccgtatgc cgcgtaccat tcggggaaat cggagacgtc gacagcgccg
     4381 caggattgcg taagacttcg gctcgggtgc gcgagattca ccagccagag taaaggtgat
     4441 ttcttacgct cgagtactaa cgctcgccaa cgctgatgct cttcatgcaa acctctgctc
     4501 gcttcgctgc cgtcatcgta ctcgccaccg gggcttgggc ctgctctgga ggcgacgggg
     4561 cttccgactc cggcagcggc ggcaacgcga acccaggcgg cgccgcgaac ggcggcgcgt
     4621 cggggaacgg tggcgcgagc ggtttcacgt cggtcgggca aggcgggaag agcggctcgg
     4681 gcggaacgaa cgccgcaggc gccgcgacga gtggcggcac gtcggcgcaa ggcgggacag
     4741 cgagcagtag tggtggtcgg agcgatggca cgagcggcgg aagcactccc atgagcggcg
     4801 gcagctcgag cgggggcaac cccggaggca catcgaatcg cggcggagcg acttcgagcg
     4861 gtggagccaa ttccggaggt tcaggcggcg ttgggaacac cgggggagct gccggtagcg
     4921 gcggcggcac ggcgggaggc gggacgagcg gcggcgtcag catctgcaag ttcgcgagcg
     4981 gcttgaatat cgcttgggtc aacttcgcga acgacgtgcc gaacccgaac atctccacgt
     5041 tcaatacgat cttcaagaac tcgcacgacg cgggcggccg tgtgattcgc tggtggtttc
     5101 acacgaacgg aacgaagacg ccgggctacg actcgagcgg catggccatg aagctcgtgc
     5161 agtcacacat cgacggagtc aaggcgatcc tctccgccgc gcagcaaaat ggcgtgcgcg
     5221 tgacgctgag cctttggtcc ttcgacatgc tgcaggacaa cgcgatgaca gcgtggacga
     5281 acaatcaaaa gctcctcgag gtcgatgaaa atcgacaggc ttacatcgac aactatctca
     5341 tccccctcgt gaacgcgatc aaaggccacc ccggcctata cgcctgggag atcttcaacg
     5401 aaccggaagg gatgggaccg aagggttggg cgacgcaccg aaccacggaa gccgccattc
     5461 aaaagacggt gaactggttc gctgccgcaa ttcacaccgc ggatcccagc gcgctagtca
     5521 cgaacggtgc ccagaccttc gactactg
//
LOCUS       DASTKS010001010         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1276950, whole genome shotgun sequence.
ACCESSION   DASTKS010001010 DASTKS010000000
VERSION     DASTKS010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276950"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFQ35_21375"
     CDS             complement(<1..352)
                     /locus_tag="VFQ35_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933276.1"
                     /translation="MRDRIREGFEKLAVVATLGAMAAASATSCSAGSEAERVERVEQG
                     LGAPVTVSFQNGVAPSAAYAGTNDTSLKQASATTNFGSQTTLYVDGDDGSGVDLSTLI
                     SWSLSGIPAGSVVQS"
     gene            complement(461..1516)
                     /locus_tag="VFQ35_21380"
     CDS             complement(461..1516)
                     /locus_tag="VFQ35_21380"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9933277.1"
                     /translation="MSRLDRRQFVVLLGALGNPACHARPRREAALRALVEELALSDVN
                     LLASRSADLRRSAERLSSLPSSAELGALRAAWRRAAHAWKRASAFRWGPLLESGALAR
                     ANYWPARLAAVDALLDGPREVTRELVDELGADVKGVYALEYLLFEPEGPSRLLPELDV
                     RGAHRARDLVRIYAEDVDAQALRARRAFVATSRSFADSLVQRPTEALARIATMLVENV
                     EALLIARFRTALWLAALSRLEASAIEGEASGISHELVLTLLSSTHRLYRGAVFPGVAD
                     LVRSSAPRVHEHVEALFARCIAIVRALPAIGEAARRRRPNLEAANSALKALETALRSE
                     LPNALGVSLAFTGLDAD"
     gene            2043..>2505
                     /locus_tag="VFQ35_21385"
     CDS             2043..>2505
                     /locus_tag="VFQ35_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933278.1"
                     /translation="MRLRLSRSLGSFVIATVVACASGTYDGDPNAQGGSLSNGGKAAS
                     GGSPSAGGASAGGPSSGGNAQAGSSATSSGGTGTSGGGSSRGGNNSGGQAGAGGSSSG
                     GSSSGGSSSGGASSGGNSNRGGSSSGGASNGGGAQGGSTARGGSSNAGGAQG"
BASE COUNT          442 a          838 c          833 g          392 t
ORIGIN      
        1 cggactgaac gacgcttccg gcagggatcc cggacaagct ccaagagatg agcgtcgaca
       61 agtcgacccc actcccatcg tcgccgtcca cgtagagagt ggtctgcgaa ccgaagttcg
      121 tggtcgcgga ggcttgcttc aacgaggtgt cgttggtgcc cgcgtaggcc gcgctcggcg
      181 caacgccgtt ttgaaaagat acggtgaccg gagcgccgag gccttgctcg acgcgctcga
      241 cccgctctgc ttcgctaccg gcgctgcacg acgtcgccga tgccgcagcc atcgccccca
      301 aagtcgccac gacggcgagc ttctcgaagc cttcccgaat gcggtccctc atgtcttctt
      361 cctccgtttc gcgaccaggc tgattcgaac cgcgctgatg gatagagggc gaaggtgaag
      421 cgaacggctc cgttcggtga cgtttctgct cggcgcgtcg ttaatccgcg tcgagcccgg
      481 tgaacgccag gctcacgccg agggcattcg gcaattcact gcgcagcgct gtctccaacg
      541 ccttgagagc ggaattcgcc gcctcgaggt tcggacgtcg cctgcgcgcc gcctccccga
      601 tcgcagggag ggctcgcacg attgcgatgc aacgcgcgaa cagcgcctcg acatgctcgt
      661 ggacgcgcgg tgccgaagag cgcaccagat cggcaacgcc tggaaagacc gccccccgat
      721 agagccggtg ggtcgagctc agcaacgtca ggacgagctc gtgagagatc ccgctcgcct
      781 caccttcgat ggcagacgcc tcgagtctgc tgagggcggc gagccaaagc gcggttcgga
      841 accgcgcgat gagtagagct tcgacgttct cgacgagcat ggttgcgatg cgcgccaatg
      901 cttcggtcgg acgctgcacg agcgagtcgg cgaagctcct cgacgtagcg acgaacgctc
      961 gacgcgcccg caaggcctga gcatcgacat cttcggcgta aatgcgaacg aggtcccgag
     1021 cacggtgggc gccgcgcaca tcgagctcgg gcaagagccg cgacggacct tctggctcga
     1081 acaacaaata ttcgagcgcg tagacgcctt tgacgtcagc gccgagctcg tcgacgagct
     1141 cacgggtcac ctctcgaggc ccatcgagca gagcgtcgac cgccgccagt cgcgcaggcc
     1201 agtaattggc gcgcgccaac gcgccgcttt ctagcaaagg tccccaacga aacgccgatg
     1261 cccgcttcca agcgtgcgcg gcccgacgcc aggccgcacg cagcgccccg agttcggcgc
     1321 tcgaaggcaa cgacgaaagt cgctcggcag agcgccgcaa atcggcgctc cgcgaggcga
     1381 gcaggttcac gtcggacagc gcgagctctt cgactagcgc gcgcagcgcg gcttctcgcc
     1441 gcggccgtgc atggcaagca gggttcccca gcgcaccgag caggaccacg aactggcgcc
     1501 ggtcgaggcg gctcatgctc cgagcgcttg tcgcccgagg tggtgacggt tcggtcgcgg
     1561 cgctgaaact ttcgggcgac gcaccggacg agaccgcctt gagaacgccc gcctcaaccg
     1621 cgtctcagga ctcgtcacct gtcgcgagca ggtgggcctt tgtctcgttc gctcgcagca
     1681 ggctcggcaa gaaagagctg tttcccaacc cgcgcttcga aagagtccgt tccgaatgct
     1741 ttggtttgtg tgggtttcgg ggccgcagaa ccccgaaagc ggcccatgcg tggttcggag
     1801 acccgatgaa tgaggagacc cgcgtagccg tcgcgcgaag cctgcctggt ccgccgccgt
     1861 cgcatttact attcgcagga gcgcacacga attcaacggc tgagcaaatc ttcttcgacg
     1921 cacgcgtgcc gaatagaacg ccttttcggc acgcgtggtg tcgcatcacg tccgggcgaa
     1981 gcgcggtcgg aatcggttcc caagcccgtc cgctctgttg ccggtgcaac ctgtaagatc
     2041 cgatgagact tcgtctttcc cgtagccttg gttccttcgt catagcgacc gtagtcgcct
     2101 gcgcatctgg aacgtacgac ggtgacccca acgcgcaggg cggctccctc agtaacggcg
     2161 gaaaggctgc ttcgggtggc tccccaagcg ccggcggcgc gtcggcggga ggcccttcgt
     2221 cgggcggtaa cgcacaggcc ggatcttctg ccacatcgtc gggtggaacc ggtaccagcg
     2281 gcggaggctc gtcgcgcggc gggaacaact caggcgggca ggcgggcgcg ggcggcagct
     2341 cgagcggcgg cagctccagc ggcggcagct ccagcggcgg cgcgtcgagt ggcggcaact
     2401 ccaaccgcgg cggcagctcc agcggcggcg cctcgaatgg cggcggagct caaggcggaa
     2461 gcacggcgcg cggcggcagc tccaacgcgg gcggcgccca aggcg
//
LOCUS       DASTKS010001011         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1501466, whole genome shotgun sequence.
ACCESSION   DASTKS010001011 DASTKS010000000
VERSION     DASTKS010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501466"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..783
                     /locus_tag="VFQ35_21390"
     CDS             163..783
                     /locus_tag="VFQ35_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933279.1"
                     /translation="MRLAARAERLVIGLACYAVLRCVLGCSGQDYYPNVGVGGSSGGA
                     PGAGGASGGAPTGANGGITGSSGAPSGGVSASSGGASGGTDAGTGGCANTGVYRTETM
                     SPGVDCSGCHVFKVSGTVYPRGDVPSFCDGTPSISIEVTGSDGEVVTLVSNRVGNFES
                     DRPLSPPYGVVVRSGNATRTPTHVTAGNCNGCHTSADTGPGRILAP"
     gene            complement(929..>2441)
                     /gene="lon"
                     /locus_tag="VFQ35_21395"
     CDS             complement(929..>2441)
                     /gene="lon"
                     /locus_tag="VFQ35_21395"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9933280.1"
                     /translation="YLDWILDLPWSHSTEDNMDITEVRRVLDEDHYGLEKVKKRIVEY
                     LAVRKLKKDKKGPILCLIGPPGVGKTSLGRSIARALGRKFVRVSLGGVHDEAAIRGHR
                     RTYVGALPGQIIQGMKKSGTVNPVFMMDEVDKIGHDFRGDPSAALLEVLDPEQNNTFA
                     DHYLEIPYDLSSVMFVATANVADPIPPPLRDRMEILEIPGYTRREKLAIARQHLIPKQ
                     LEEHGLKPEQLTITDEAVEELIEHYTREAGVRSLERTVAGVIRGVAVKVAEGDSTARV
                     IKSEDDLREFLGPIKYTSEVAERTEETGVATGLAWTSVGGEILFIEVTRMFGSGKMQL
                     TGQLGDVMKESAHAALSYVRTNAEKYGIAKDFLEKSDIHIHIPAGAMPKDGPSAGITM
                     FTAIVSLLTGARVRHDVAMTGEISLRGRVLPIGGVKEKTLAAHRAGIKRVILPERNKA
                     DLEEVPKEVRDELEFVFVHKLDEVLEAALEKMPQPSQAYLDEVAKREAPAPTN"
BASE COUNT          392 a          814 c          785 g          450 t
ORIGIN      
        1 aaatccgctg ccgaacggcg tagccagcct gcgccagact gggccacccc cgggttccac
       61 cgtcgcgctc tagggggcat tttcgccctg cttcgacgaa ggagcgaacc cggagtttcc
      121 aatgcaacgc ccccggcata cgcgttgctt gagttcggca gaatgagact cgccgcgcgc
      181 gcagaacggc tcgtcatcgg cctcgcatgc tatgcggtgc tgcgttgcgt gctgggttgc
      241 tcgggtcagg actactaccc gaacgtcggc gtcggtggct cgagcggcgg agcacctggc
      301 gcaggcggcg cgagtggcgg agcgccaacc ggtgcaaacg ggggcataac cggaagttcg
      361 ggcgcgccta gcggcggagt gtcagcgagt tcgggtggtg cgagcggcgg aaccgacgca
      421 ggaaccggcg gctgcgccaa taccggcgtc taccgcaccg agacgatgtc tcccggcgtc
      481 gattgctcgg gctgtcatgt cttcaaggtc agcggcaccg tctacccgcg cggggacgtg
      541 ccgagcttct gcgacggaac cccttcgatc tcgatcgagg taaccggctc cgacggcgaa
      601 gtggtgaccc tcgtttcgaa tcgcgtcggg aatttcgagt ccgatcgacc cctgtctcct
      661 ccctacgggg tcgtcgtgag aagcggcaat gcgacccgaa cgccaaccca cgtcacggct
      721 ggcaactgca acggctgcca cacgagcgcg gacacggggc ctggccgaat cctcgcgccc
      781 tgaactcggg ctcgctgcgc tttcagcact tccaagtcct catcacgcag gaacaaaagc
      841 gcgaagcgcc gagaacccga aggctcccag cgcttctgaa cgcggccccg ccagtgtatc
      901 ggcagggccg agccgcgctc cgaccaaatc aattcgtcgg agctggtgcc tcgcgcttcg
      961 ccacctcgtc gaggtacgcc tgcgacggtt gcggcatctt ctcgagcgcc gcctcgagca
     1021 cctcgtctag cttgtgcacg aagacgaact cgagctcgtc acggacctcc ttcggcacct
     1081 cttcgagatc cgccttgttg cgctccggca agatgacgcg cttgatgccg gcccggtgcg
     1141 cggcgagcgt cttctccttc acgccgccga tcgggagcac gcggccccgc agcgagattt
     1201 cgcccgtcat cgcgacgtcg tgtcgcactc gcgcgcccgt gaggagcgac acgatcgcgg
     1261 tgaacatcgt gatgccggcg ctcggcccgt ctttcggcat cgcgcccgcg gggatgtgga
     1321 tgtggatgtc gctcttctcg aggaaatcct tggcgatgcc gtacttctcg gcgttggtgc
     1381 gaacgtagct gagcgccgcg tgcgcgctct ctttcatgac gtcgccgagc tgcccggtga
     1441 gctgcatctt gccggacccg aacatgcgcg tgacttcgat gaagagaatc tctccgccga
     1501 cgctcgtcca cgcgaggccc gtcgcgacgc cggtttcctc ggtgcgctcc gcaacttcgc
     1561 tcgtgtactt gatgggcccc aagaactcgc ggaggtcgtc ctcgctcttg atgacgcgcg
     1621 ccgtggagtc accctcggcg accttgacgg caacgccgcg gatcacgcct gccaccgtgc
     1681 gctccaggct gcgcacgccg gcttcgcgcg tgtagtgctc gatgagctct tccaccgcct
     1741 cgtcggtgat ggtgagctgc tcgggcttca gtccgtgctc ttcgagctgc ttcgggatca
     1801 agtgttgccg cgcgatggcg agcttctcgc ggcgcgtgta ccccgggatc tccaggattt
     1861 ccatgcggtc ccggaggggc ggcgggatgg ggtcggcgac gttggcggtc gcgacgaaca
     1921 tcacactcga gaggtcgtac gggatctcga ggtagtgatc ggcgaaggtg ttgttctgct
     1981 cggggtcgag cacctcgagc aacgccgcgc tcggatcgcc gcggaagtcg tgcccaatct
     2041 tgtcgacctc gtccatcatg aacaccgggt tcacggtgcc ggacttcttc atcccctgga
     2101 tgatctgccc cggcagcgcg ccgacgtagg tacgacggtg accgcgaatc gcggcctcgt
     2161 cgtgtacacc gccgagcgag acacgcacga acttgcgacc gagagcgcgc gcgatgctgc
     2221 gaccgagcga cgtcttaccg acgccgggcg gaccgatgag gcaaaggatc ggccccttct
     2281 tgtccttctt gagcttgcgc acggccaaat actcgacgat gcgcttcttg accttctcga
     2341 gcccgtaatg gtcctcatcg agtacgcggc gcacctcggt gatatccatg ttgtcctcgg
     2401 tcgagtgcga ccaaggcaaa tcgagaatcc aatccaggta g
//
LOCUS       DASTKS010001012         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_829317, whole genome shotgun sequence.
ACCESSION   DASTKS010001012 DASTKS010000000
VERSION     DASTKS010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829317"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..469)
                     /locus_tag="VFQ35_21400"
     CDS             complement(125..469)
                     /locus_tag="VFQ35_21400"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET9933281.1"
                     /translation="MATDPQDFAKEFSEGGFWEKVRGFARAAGEAVLEPALKLYYASQ
                     DSDTPPWAKAVIYSALGYFIAPFDAIPDVTPIVGYADDLGVLVAALATVAAHIKPAHG
                     ERAKETLRQWFG"
     gene            complement(572..1648)
                     /locus_tag="VFQ35_21405"
     CDS             complement(572..1648)
                     /locus_tag="VFQ35_21405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933282.1"
                     /translation="MAPRSTAQHPPAASLVSTQRLHDCIAEAPRSSSDVRARRKRERV
                     REAEVASQVALRTTYDDAPERSESRLRGQIVAGKYRLIEPLARGAMGTIWRAEHVLLN
                     SLVALKLIDDSAPFGSVAQLRFLREARTAARLRGAHVVELLDYGLDGERAYLVMELLA
                     GESLRERLVRRGSLSKRETARIVTQLARALERARRFSIVHRDLSPSNVFLERADASIS
                     VKLLDFGIAKWLSESDAFAPNLTVSGAILGTPSYMSPEQLEASSSLDWRADIWALGVI
                     AFECLLGHVPFRGDTFPALVVAICSRPLPVPSELGRVPAAFDAWFAKACARSPKERFG
                     SASEAALELRRSLGCAGRPRSRSR"
     gene            complement(1865..>2059)
                     /locus_tag="VFQ35_21410"
     CDS             complement(1865..>2059)
                     /locus_tag="VFQ35_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933283.1"
                     /translation="CAVQIADNHEPPAPASSHLHRASVASVQWSLASLWDVRREPSAS
                     QACRLVRRSDDAHAVIDESP"
BASE COUNT          403 a          643 c          684 g          329 t
ORIGIN      
        1 agatgcgccg ttgcaagcgc gcgggcacgg agcccggcat gattgaccgg ggatgcgagt
       61 cgattccgcg ctggacgatg cgcaccgcat tcgagactgc tctgaaccat gggcgcgagc
      121 gagctcagcc aaaccactgc cgcaatgtct ctttggcgcg ctcgccatgt gcaggcttga
      181 tgtgagccgc gacggtggcg agggcagcga cgagcactcc gagatcgtcc gcgtacccga
      241 cgatgggcgt gacgtccgga atggcatcga acggcgcgat gaagtaaccg agcgcgctgt
      301 agatgacggc tttggcccac ggcggcgtgt cggagtcttg cgaggcgtag tagagcttga
      361 gcgccggttc gagcacggcc tcgcccgccg ctcgcgcaaa gccgcgcacc ttttcccaaa
      421 agccgccctc cgaaaactcc tttgcgaaat cttgcgggtc ggtggccatc gctcaagtat
      481 gcggccggag cgctgcaagg caatgatgcg cctgtttcgc aaccggcgcg cccactgcgg
      541 cttctcctgc gatgctcgca aaagcgccac gtcagcggga acgagagcgg gggcgacctg
      601 cgcaaccgag cgagcgccgg agctcgagcg cggcttcgct cgcggagccg aagcgctctt
      661 tcggcgagcg agcgcacgcc ttcgcgaacc acgcgtcgaa cgcggcggga acgcgcccaa
      721 gctccgaagg cactggcaac ggacgcgaac agatcgctac gacgagcgcc ggaaacgtat
      781 cgcctcgaaa ggggacatgg ccgagcaagc actcgaacgc gatgacgccg agggcccaaa
      841 tgtcagcgcg ccaatcgagt gacgaggatg cctcgagctg ttccgggctc atgtagctcg
      901 gcgtgccgag gatggctccg ctcacggtga ggttcggcgc gaacgcatcg ctttcggaga
      961 gccactttgc gatgccgaag tcgagaagct tgaccgaaat cgaggcgtcg gcgcgctcga
     1021 ggaacacgtt gctcggcgaa agatcgcggt gcacgatgga aaatcttcga gcgcgctcga
     1081 gcgctcgcgc aagctgagtc acgatgcgag cagtctcgcg cttggagaga gagcctcgac
     1141 gcacgagtcg ctcgcgcagt gattcgcccg cgaggagctc catcaccagg taagcgcgct
     1201 caccgtccaa accgtagtcg agcagctcga cgacgtgcgc acctcgaaga cgagctgctg
     1261 tcctcgcttc acgcaagaag cgcagttgtg ccacgctgcc gaacggcgcc gagtcgtcga
     1321 tgagcttcag cgcgacgaga gagttcagta gcacatgctc ggcgcgccaa atcgttccca
     1381 tggcgcctct cgccaacggt tcgatcagcc ggtatttgcc ggcgacgatc tgcccccgta
     1441 atcgggactc ggaccgttcc ggagcgtcgt cgtaagtggt gcgaagggca acctgtgatg
     1501 caacttcggc ctcgcgcact cgttcgcgct tgcgacgcgc gcgaacgtcg ctggagctcc
     1561 gtggagcctc cgcgatgcaa tcgtgtaacc gctgcgtcga gaccaggctc gcggcgggag
     1621 gatgctgggc agtcgatcta ggagccatgc gggcgcctcg attggaaatg agcgaactcg
     1681 tggaaggctc ttcgcgttga cgagaagaac cgcagaaatc ctcgagcttc gcgcggtttc
     1741 cagcgagcgc ctcgcggcgc cgcgactaga ccgacttcaa aacgaaggac gaggagagag
     1801 cacggcagcg actggattga tgtttcaccg aaaactcctc ctttcgccga gcagtttgcc
     1861 aggctcacgg cgactcgtca atcaccgcgt gcgcatcgtc gctacgacgt actaaccggc
     1921 aagcctgtga cgccgacggt tcgcgccgga catcccataa agaagcgagc gaccattgca
     1981 ccgatgctac gctggcccgg tgaaggtgcg acgaggcggg cgcgggcggc tcgtggttat
     2041 cggcgatttg aacggcgca
//
LOCUS       DASTKS010001013         4545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1053282, whole genome shotgun sequence.
ACCESSION   DASTKS010001013 DASTKS010000000
VERSION     DASTKS010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053282"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFQ35_21415"
     CDS             <1..625
                     /locus_tag="VFQ35_21415"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9933284.1"
                     /translation="LPAEPGESFAWRGLLPDSQQVLFGKSVDRFGGERDFELRFRTLP
                     AAPHLVLNEALSNALGAEPASEWIELFNDGPAPAELGGLRIADGGGQVELPPGRLEPG
                     TYALLVRDDFVENGVDVPPAPGTTIVRLPSLGKNGLSNSGEALTLLDANDRVLSRMPA
                     LPSAHAGVSLARRTPASPDDAPDSFAEHAAPGASPGAENSVVAAAAR"
     gene            complement(649..2010)
                     /locus_tag="VFQ35_21420"
     CDS             complement(649..2010)
                     /locus_tag="VFQ35_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9933285.1"
                     /translation="MQDREAAELEALGADVVERARRAGAEVAEVSARRGFDLSVRVRL
                     GNTELVEEAGQRGLSLRVISGQRVALASTSDFTEAGIRRLVADAIELASLSEPDPFAG
                     PADPSELCSAPHPDLDQYDPRIAEIAAGEAIGVALACEKAALGSDQRITLSEGATFSR
                     STGVSAMVLSSGFSATQRGSYASLSVAPVVEDEGGKKRRGHYWTASCYRDGLEPAEAV
                     GQEAARRTLRKLGARKVPTTETPVVFDPDVSRSLLSAFAGCIMGGAIWRKSSYLVERE
                     GTVVASPLVTIVDDPLIPRAFGSRAFDGEGLRSRRNVVVDGGTLKTFLLDSYSARKLN
                     KRSTASAVRSGGVGPSTSNFILQAGAMSAEELIQNTASGLYVTEMMGFGFNALTGDFS
                     RGAGGFWIENGKFAFPVSEVTLSSNLDAMLKGIDAIASDLVLKTSTAAPTLRVNRMTL
                     SGT"
     gene            complement(2082..2621)
                     /locus_tag="VFQ35_21425"
     CDS             complement(2082..2621)
                     /locus_tag="VFQ35_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933286.1"
                     /translation="MPAPIGPLIGLALGALFAWIASDGLPRSKASLALPPLALVSLFA
                     LLVFAPACAYFIAFEPDWAYAYLVDGSRRLGALHAAVLLCDVVSLPLGFVLLVYSAHG
                     PTFAQMLRLLAAPLLASAVFVVVMFPRLSVQATFAQFHGDFGTRAVAGGPLGYALIWT
                     TLVLCGAFAWTAHALRRMR"
     gene            complement(2626..3441)
                     /locus_tag="VFQ35_21430"
     CDS             complement(2626..3441)
                     /locus_tag="VFQ35_21430"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9933287.1"
                     /translation="MLSIDALLDRVVEVCPLSATTQRVLALTSGDTASIPEVARVIST
                     DPALAAAVLSVANSAAYGMGKVDRLEVAVMRLGLRELHNLAAAMALFAAFRSKAELSL
                     QLHELCVASGSLAHRIAKETGVAQPGSAFVCGLLSEIGAMACLVFDGKPYVELFRTSG
                     ADIDLRAQREVERYGASSFAIGQRFLERNSLPESVCYAVGSDVTAPQDWEPLAKITLL
                     ARHAPLLQRVHAADRGALDAALDALAGRIALENVDGARLRDMCKSSRAADALD"
     gene            3510..4376
                     /gene="larB"
                     /locus_tag="VFQ35_21435"
     CDS             3510..4376
                     /gene="larB"
                     /locus_tag="VFQ35_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET9933288.1"
                     /translation="MAEIPRYTPRHVCFAGPPHHGCRTAMNPDELRSLLEAVRDGSES
                     IDGAYRALAKLPFSELGYAKVDHHRALRQGFPEVVFGAGKQASQIIGIAQHMLGAGEN
                     VLVTRVSVEVADEVVRELPNLEHNALARTLSCIQKSPPTYVTEPVAVVTAGTSDVPVA
                     EEALETLRSCGVPAIRVFDVGVAGIHRLLGRLEVLNRAPAVIVIAGMEGALPSVVGGL
                     LAAPVVAVPTSVGYGTALSGFTALFGMLTSCASGVTVVNIDNGFGAALAVARLLSHCK
                     RAPAVSEPAHER"
     gene            4366..>4545
                     /locus_tag="VFQ35_21440"
     CDS             4366..>4545
                     /locus_tag="VFQ35_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933289.1"
                     /translation="MSDEPKRKLVPAAESSSFLRAPAQQHGHSHEHGHSHEHGHSHEH
                     GHSHEHGHSHEHGHSH"
BASE COUNT          780 a         1482 c         1544 g          739 t
ORIGIN      
        1 gctccctgca gagcctgggg agagcttcgc gtggcgcggg ttgcttcccg attcacagca
       61 ggtgctcttc ggtaagagcg tcgatcgttt cgggggcgaa cgtgacttcg agcttcggtt
      121 tcgcactttg ccggccgcgc cgcacttggt gttgaacgaa gccctctcca acgcgctcgg
      181 cgcggagccc gcgagcgaat ggatcgaact cttcaacgac ggcccagcgc cggccgagct
      241 cggaggatta cgcattgccg acggcggcgg gcaggtcgag ttgccgcctg ggcgcttgga
      301 gcccggaacg tacgcgctcc tggtgcgcga cgacttcgtg gagaacggtg tcgacgtgcc
      361 gcccgctccg gggacgacga tcgttcggct gccttcgctc gggaagaatg ggctatcgaa
      421 ttcaggcgaa gcgctcacct tgctcgacgc gaacgaccgc gtgttgtcgc gcatgccggc
      481 gctgccgagc gcgcatgcgg gggtgagcct cgcccgccgg acgccggcat cgccggacga
      541 tgcccccgac tcgttcgcgg agcatgcggc gcctggcgct tctccgggcg ccgaaaactc
      601 ggtcgtcgcg gcagctgctc gatagcaggg gtcgcgtcga gcggttcgtt acgttccgga
      661 cagggtcatc cggttgacgc gcaaggtcgg ggcggcggtg ctcgttttga ggaccaggtc
      721 actggcgatg gcgtcgatgc ccttcaacat cgcgtccaag ttcgacgaga gcgtcacttc
      781 gctcactggg aacgcgaatt ttccgttttc gatccagaac ccacccgcgc cgcgcgaaaa
      841 atctccagtg agcgcgttga agccgaagcc catcatttcg gttacgtaga gccccgacgc
      901 cgtattctgg atcagctcct cggcggacat ggcccccgct tgcaagatga agttgctcgt
      961 cgatgggcct acgccgccgc tgcgcactgc ggatgcggtc gaacgcttgt tgagcttgcg
     1021 agcgctgtag ctatcgagga gaaatgtctt gagcgtgccg ccgtcgacga cgacgttgcg
     1081 gcgtgagcgg aggccctcgc catcgaaagc gcgcgagccg aaggcgcgag gaatcagcgg
     1141 atcgtcgacg atcgttacga gcgggctcgc gacgacggtg ccttcacgct cgaccaagta
     1201 cgacgacttg cgccagatcg cgccgcccat gatgcacccc gcgaacgcgc tgaggagcga
     1261 gcgcgagaca tcgggatcga atacgactgg cgtttcggtg gtcggcactt tgcgcgcgcc
     1321 gagcttgcgg agcgtgcgtc gcgcggcctc ttgccccacc gcttctgccg gctcgaggcc
     1381 gtcgcgatag cagctggcag tccaatagtg gccgcggcgc ttctttccac cttcgtcttc
     1441 gacgaccggg gcgacggaga gcgaagcgta agagccgcgc tgcgtcgccg agaagccgct
     1501 cgacagcacc atcgcagaga cgccagtgct gcgcgaaaat gtcgcgcctt cactcaaggt
     1561 gatgcgctga tccgacccga gcgcggcctt ctcgcaggcg agcgcgacgc cgatggcctc
     1621 tcccgccgcg atctcggcga tgcgcggatc gtattgatcg agatccgggt ggggagcgct
     1681 gcagagctcg ctcgggtccg cggggccggc aaacggatct ggttcgctca agctcgcgag
     1741 ctcgatggca tcggcgacca gccgacgaat gcccgcttcg gtgaaatcac tggtggaggc
     1801 gagtgcgacg cgctgcccac tgatgactcg cagcgacaag ccgcgctgtc ccgcttcttc
     1861 gacgagctcg gtgttgccga ggcggacacg cacggacaaa tcgaacccgc ggcgggcgct
     1921 cacctcggcg acttccgcgc ctgcccggcg tgctcgctct acgacgtcgg cgccaagggc
     1981 ttctagttcg gcggcttctc gatcttgcat ggtcgtcccg tacacttagc acgaagcgtt
     2041 aggccgatgc gcccgccaaa agtccgaatt cttagcggaa ctcagcgcat tcgtcgcaag
     2101 gcgtgggcgg tccaggcaaa ggcgccgcac agcacgagcg tcgtccaaat caaggcgtag
     2161 ccgagcggac cgccagccac cgcgcgcgtc ccgaaatccc catgaaattg tgcgaaagtg
     2221 gcctggacgc tcaaccgcgg aaacatgacc acgacgaaca cggcgctcgc caagagtggc
     2281 gccgccaaga gccgcaacat ctgcgcgaag gtcgggccgt gtgcggagta caccagcagc
     2341 acgaagccga gcggtaagct caccacgtcg cacaagagaa cggccgcgtg gagcgccccg
     2401 agcctgcggc ttccatcgac gagataggcg tatgcccagt ccggctcgaa cgcgatgaag
     2461 tacgcgcacg ccggcgcaaa cacgagcagc gcgaacaggc tcacgagcgc gagcggcgga
     2521 agcgcgaggg aagctttgct gcgcggcaac ccgtcgctgg caatccaagc gaagagagcg
     2581 ccgagcgcga ggccgatcaa cggaccgatg ggagcaggca ttgtttcaat cgagggcgtc
     2641 ggctgcgcgg gagctcttgc acatatctcg caaccgggca ccgtcgacgt tttcgagcgc
     2701 gatgcgcccc gcgagagcat cgagggccgc atcgagagcc cctcggtctg cggcgtgaac
     2761 gcgctggagc agtggggcgt ggcgcgcgag cagcgttatc ttggcgaggg gctcccagtc
     2821 ttgcggggcc gttacatcgc tccccacggc atagcagacg ctttcgggta gcgagttgcg
     2881 ttcgaggaaa cgctggccga tggcgaagct ggaggcgccg taacgttcga cttcgcgttg
     2941 cgcgcgcagg tcgatgtcgg cgccgctcgt tcggaacagc tcgacgtacg gctttccgtc
     3001 gaagacgagg catgccatcg cgccgatttc ggagagcagc ccgcacacga acgcgctgcc
     3061 gggctgggcg actccggtct ccttcgcgat gcgatgcgcg agcgaccccg aagcgacgca
     3121 aagctcatgt agttggagcg agagctccgc cttgctgcga aaggcagcga acagcgccat
     3181 ggccgcggcg aggttgtgga gctcgcgtag cccgagccgc atcaccgcga cctcgagccg
     3241 gtcgaccttt cccatcccat aggcggcgct gttcgcgacg ctgagaaccg ccgctgccaa
     3301 cgcgggatcg gtggagatta cgcgggctac ttcaggaatg ctggccgtgt cgcccgaggt
     3361 caaggcgagt acgcgctggg tggtggccga cagggggcag acctccacga cccgatcgag
     3421 caaggcgtcg atgctgagca ttctctgtca cagcgtagcg aagttgcgcc ccgctcgcga
     3481 gcgtttcggc gcgcgccctg gcgatcgaca tggctgaaat tccgcgctac accccccgcc
     3541 atgtgtgctt cgccggtcct ccccaccacg ggtgccgcac cgcgatgaat cccgacgagt
     3601 tgcggtcctt gctagaagcg gtgcgcgatg gctccgagag catagacggt gcctatcggg
     3661 cgctggcaaa gctcccattt tccgagctcg ggtacgccaa ggtcgatcac caccgcgcgc
     3721 tgcgtcaggg atttcccgag gtggtgttcg gggctggcaa gcaagcctcg cagattatcg
     3781 ggatcgccca gcacatgctc ggggccggtg agaacgtgct cgtcacgcgc gtctcggtgg
     3841 aggtcgcaga cgaggtcgtg cgcgagctgc cgaacctcga gcacaacgcg cttgcacgta
     3901 cgttgagctg cattcaaaaa agcccgccga cctatgtcac cgagcccgtc gcggtcgtca
     3961 ccgccggcac gagcgatgtc ccggtggcgg aggaggcgct cgagacgctc cgctcgtgcg
     4021 gcgtgcccgc gatccgcgtc ttcgatgtcg gagtagcggg gatccatcgc ctgctcggcc
     4081 gcctcgaggt gttgaatcgc gcgcccgcgg tcatcgtgat cgccgggatg gagggcgcac
     4141 tgccgagcgt cgtgggcggg ttgttggcgg cgccggtcgt ggcggtgcca acctcggtcg
     4201 gctacgggac cgcgctctcg gggttcaccg cgctcttcgg catgctgacg agctgcgcgt
     4261 cgggtgtcac cgtcgtgaac atcgacaacg gcttcggggc ggccttggcg gtcgcgcggc
     4321 tgctctcgca ctgcaagcga gcccccgctg taagcgagcc cgcgcatgag cgatgagccg
     4381 aagcgcaagc tcgtacccgc ggcggaatcg agctcgtttc tccgcgctcc cgctcagcag
     4441 cacgggcact cacacgagca cgggcactca cacgagcacg ggcactcaca cgagcacggg
     4501 cactcacacg agcacgggca ctcacacgag cacgggcact cacat
//
LOCUS       DASTKS010001014         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_383081, whole genome shotgun sequence.
ACCESSION   DASTKS010001014 DASTKS010000000
VERSION     DASTKS010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383081"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFQ35_21445"
     CDS             <1..470
                     /locus_tag="VFQ35_21445"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9933290.1"
                     /translation="DDRHWRAVPCSLKEPAMSPADSDNPTRVTDPDTRTDTRQTAILI
                     FRDEGVDWPQLAADGRSKMLFQKLVDWAADLKRRGLYAGVEALAQSGKTVRRKGASIV
                     IDGPFAEGREAVLGFFLVRVRDLDEACAIAGESPHAEVGGATEVRLLGTFPKP"
     gene            complement(498..1694)
                     /locus_tag="VFQ35_21450"
     CDS             complement(498..1694)
                     /locus_tag="VFQ35_21450"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HET9933291.1"
                     /translation="MVDLDALYREHAPAITASLVRSFGPRRLDLIETAVQEAFVSAIE
                     SWSENVPDRPGAWLQVAARRRVIDAIRRSTWVTPSDALDELEAPAPVASRDEDLLKMM
                     FVCCHPAIPLESALALALRTLCGFPVPALSRALRLDEAAIEKRLSRARQVLREEHVEL
                     EPDGELSMRVDGILYTLYVLFLEGYSAHAGDAQIDEDLCRTAIRLNAALLASQFATPR
                     GHALQALFLLQAARLTSRVDAAGNLIPLDRQDRARWDRTMIADGLEHLGRAATGVVVS
                     PFHLEAGIAACHALAPTYAATDWQQIVAFYDQLLALTPSPVIALQRAIAVGRANGARA
                     GLRALERLVGNDRLEDDPVLAAAIGELEAQRGDVRAARTAYRRAIELAGTAPERRFLE
                     ERLAAL"
     gene            complement(1906..>2113)
                     /locus_tag="VFQ35_21455"
     CDS             complement(1906..>2113)
                     /locus_tag="VFQ35_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933292.1"
                     /translation="LAGELYSARFYGALHRVLTRNGKLFHYIGDANSASGARTTRGVV
                     KRLRDAGFSRVTVLEEAFGVLANA"
BASE COUNT          383 a          701 c          723 g          306 t
ORIGIN      
        1 acgacgaccg gcactggaga gccgtaccct gcagcctcaa ggagcccgca atgagccccg
       61 ccgacagcga taaccccacc agagtgacgg accctgatac tcgcaccgac acgcgacaga
      121 ccgcgatact gatctttcgc gacgagggcg tcgactggcc ccagctcgca gccgacgggc
      181 gatcgaagat gttgttccag aagctcgtcg attgggccgc cgatctcaag cggcgcgggc
      241 tgtacgctgg cgtcgaggcg ctcgcgcaga gcggcaagac ggtgcgccgc aagggtgcaa
      301 gcatcgtgat cgacggtccg ttcgcggagg ggcgcgaggc ggtgctcggc ttcttcttgg
      361 tgcgcgtccg cgacctcgac gaggcgtgcg cgatcgccgg agaatcgcct cacgccgaag
      421 tcggcggcgc gaccgaggtg cggctgctcg gcacgtttcc gaaaccgtag tcgctcagga
      481 cgttggcacc gccccgatca aagcgccgcc agccgctctt cgaggaagcg gcgctcggga
      541 gcggtgccgg cgagctcgat ggcgcggcga tacgcagtcc gtgcagcgcg aacgtcccca
      601 cgctgggctt cgagctcgcc gatcgcagcc gcgagcactg ggtcgtcctc gagacgatcg
      661 tttccaacca gccgctcgag agcacgcaac ccagcgcgag caccgttggc tcgaccgacc
      721 gcgatcgcac gttggagggc aatgactggc gagggtgtga gcgcgagtag ctggtcgtag
      781 aacgcgacga tctgctgcca atcggtggcg gcataagtgg gcgcaagcgc gtggcacgct
      841 gcgatccccg cctcgaggtg gaatggtgag acgaccactc ccgtcgcagc gcggccgagg
      901 tgctcgagtc cgtcagcgat catcgtgcgg tcccagcgcg cgcggtcttg ccgatcgagc
      961 gggatgaggt tgcccgccgc gtcgacacgc gaggtcaacc gggccgcctg caacaagaac
     1021 aaggcttgca atgcgtgtcc gcgaggcgtc gcgaactgac tcgcgagcaa cgccgcgttc
     1081 aggcggatcg ccgtgcggca caggtcctcg tcgatctgag cgtcgccggc gtgcgcgcta
     1141 tagccctcca ggaacaacac gtagagcgtg tagaggatgc catcgacccg catgctgagc
     1201 tctccgtcgg gctcgagctc cacgtgttct tcccgcaaga cttgacgggc gcgactcagt
     1261 cgcttctcga tggcggcctc gtcgagccgc aacgcgcgac tcagggccgg gaccggaaag
     1321 ccacacagcg tgcgcagcgc gagcgccagc gcgctctcga gcggaatggc cggatgacag
     1381 cagacgaaca tcatcttcag cagatcttca tcgcgggagg ccacaggggc gggcgcttcg
     1441 agttcatcga gcgcgtcgct cggcgtgacc cacgtcgagc ggcggatggc gtcgatcacc
     1501 cgacgccgcg ccgcaacctg caaccacgca ccgggccggt ctggcacgtt ctcgctccag
     1561 ctttcaatcg cggagacgaa agcctcctgc acggcggtct cgatcagatc gaggcgccgc
     1621 ggcccaaacg atcgcacgag cgatgccgtg atcgcgggcg cgtgctcccg gtagagcgca
     1681 tcaagatcga ccacgcgccg aatctagcgc atcgagtcac ccgaagcgac cgacgacacc
     1741 actccgccat gcaactccgc tccgccctcg tctcctccga acgagcgagg gcggcctccg
     1801 cgcgagaggt ggggtttcgg atgcactcag gaatcggtga cgacgccgtt cggtctggcg
     1861 gcatcggcta gagggtcgcg caaggaggcg gggtagctgc cgaagctatg cgttcgcgag
     1921 cacgccaaac gcctcttcca aaacggtcac gcgcgagaag ccggcgtcac gtaagcgctt
     1981 gaccacgccg cgcgtcgtgc gagcgcccga agcgctgttg gcatcgccga tgtagtggaa
     2041 gagcttgccg ttgcgagtga gcacgcggtg aagggcgccg taaaagcgcg cggagtagag
     2101 ctcaccggcg agg
//
LOCUS       DASTKS010001015         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1053539, whole genome shotgun sequence.
ACCESSION   DASTKS010001015 DASTKS010000000
VERSION     DASTKS010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053539"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..471)
                     /locus_tag="VFQ35_21460"
     CDS             complement(115..471)
                     /locus_tag="VFQ35_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933293.1"
                     /translation="MDEFEAFRRRARSRHTEAHAVALGATFLSLILGAAFDAPLIAFG
                     ATGVFAVAAGIYGFRGGVRFGVGFFRGDPSTREWEQMSPTEREAHARKDGATLIAVGL
                     GLLGLAALWAMNRVIR"
     gene            complement(566..970)
                     /locus_tag="VFQ35_21465"
     CDS             complement(566..970)
                     /locus_tag="VFQ35_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933294.1"
                     /translation="MLFDIRRAFGTVRFTLVRCLSLFVIAASGCSSTEKVCTLQLLTA
                     VRVRIESLDGLPITDVRLTSPAQQNCFENSAGATDTSVEYDCYEQGGGIYTVRVTSGT
                     MTWTQSVSVAADECHVTEQKTLQFVLDSASAD"
     gene            1679..>2716
                     /locus_tag="VFQ35_21470"
     CDS             1679..>2716
                     /locus_tag="VFQ35_21470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933295.1"
                     /translation="MGAVAQPFCERIVDRYAIYDQIAVGGMAVVHLGRLLGPAGFSRT
                     VAIKRLHPQFASNPEFVAMFLDEARLAVRVQHPNVVAPLDVLVVEDELLVVMDYVSGE
                     TLSTLMRGVQPEVGAPPSIIGSILVDALYGLHAAHEAKAENGAPLDIVHRDISPQNII
                     VGVDGVARVLDFGVAKAATRSQVTKDGEIKGKISYMAPEQLKAHAIDRRTDVFAAGVV
                     LWEALTGRRLFRGDDLGQTVEKVLHGAIPSPSTLRRDVTKALDAVVLRALQRVPAFRF
                     ATARDFAIALEGATTIASASEVADWVRSIGGGNLEQKIQRVAAIESHSSSRVKVGGRG
                     PLDTPLGLRAVG"
BASE COUNT          483 a          870 c          884 g          479 t
ORIGIN      
        1 tgttcacgag ccgacaccgg gcccaaccgg gatcgggttc ggacggcgta tcggtaggac
       61 ggcaccacca gcacggattg cgataggcag gtgcggttgt cgctaacagc gctctcaacg
      121 tatcactcga ttcatggccc acagggccgc aaggccaagc agccccaagc cgaccgcgat
      181 caacgttgca ccgtcttttc gcgcgtgggc ttcgcgctcc gtcggcgaca tttgctccca
      241 ttcgcgcgta ctcgggtcac ctcggaagaa gcctacgcca aagcgcactc cgccccgaaa
      301 gccgtagatg ccagcagcga cggcgaaaac gcccgtcgca ccgaaggcga tcagcggcgc
      361 atcgaacgcc gccccaagga tcaaactcag aaacgttgcg ccgagcgcga ccgcgtgcgc
      421 ctccgtatgc ctcgaccgtg ctcgacgccg aaacgcttcg aattcgtcca ctcctccatt
      481 gtacgtgggc gcgggtcccg gtcgacgcgg tggtcgccgc gggcacttcg accggcggtt
      541 cttgaaggtc gagcgccgga cgtcttcagt cagcggaggc gctgtcgagc acgaactgca
      601 gtgttttctg ctcggtcaca tggcactcgt ctgccgcgac cgagacgctt tgcgtccacg
      661 tcatggtgcc actcgtgact cgaaccgtgt agatgccacc gccttgctcg tagcagtcgt
      721 attcaacgga ggtgtcggtc gcccctgcgg agttctcgaa gcagttttgt tgagccgggc
      781 tcgtcaagcg gacgtccgta atcggtaaac cgtcgagcga ctcgatccgg acacgtacag
      841 cggtgagcag ctggagggtg caaaccttct cggtcgagct gcatccggac gccgcaatga
      901 cgaaaagtga caggcaccgc accagagtga atcggacggt cccgaacgcg cgtcggatgt
      961 cgaaaagcat gagtaggccc aaacttgcag gagatgtcac ttcgcgagct ccgagctcat
     1021 tccggcggtg attcgcatcg tcaccccttc cgcattcgca gcgtaccacc gcgatccgag
     1081 tgatccatcg tcgccccgat ccgcgtggcc gcgggcggcg gctggcggtg catccgcaga
     1141 caggccggcg aagaatacat tcacccccgg cgccgctccg acaccctgcg gacggcgtac
     1201 gagcgcgtcc gggcgaagcc gtcactccgg ggggagcagc tgctggcgcg acgcgcgggc
     1261 ccattgcggg cgtccccgtt gcgcggatcc tttcggtcac cgctcgattt ggaaggctca
     1321 ctcacactca atcatggggg tgaggtgcag ttcggctggc gctcgacacg aggcgtcggc
     1381 tcgacacggt tctcgctgac gagcttcgga actcactgcg aagagccaag gggcgcttcg
     1441 cgcgacgact cgtgccgaaa acccctcccc gtgtttcgct gcgctttcgc tcgcggagat
     1501 gggtcgcgct acggctcgcg tggctcaggc gagacgagct cgcgtgctca ggcgaacttc
     1561 gccagtgcga gagagtgcga cgaagatgac gtcataccta ggtgtcattc gaacagtcct
     1621 cggtgagctc ggagaatcac gttctctcca tcaggtgtcc ctgttagagt gagtaacgat
     1681 gggcgcggtt gctcagccct tttgcgagcg aatagtcgat cgatatgcga tctacgatca
     1741 gatcgccgtc ggaggcatgg cggtcgtcca cttgggtcgg ttgctggggc cggccggctt
     1801 ctcgcgcacg gtcgcgatca agcggctgca cccccagttc gcgtcgaacc ccgagttcgt
     1861 cgcgatgttc ctggacgagg cgcggctcgc ggtgagagtg caacacccga acgtcgtcgc
     1921 gccgctcgat gtcttggtcg tcgaggacga gctcctggtc gtgatggact acgtctccgg
     1981 cgagacgctc tcgacgttga tgcgcggcgt acagcccgaa gtgggagcgc cgccttccat
     2041 catcggcagc atcctggtcg acgcactcta cggcttgcac gcagcccacg aagccaaagc
     2101 cgagaacggc gctccactcg acatcgtgca tcgcgacatc tcgccgcaga acatcatcgt
     2161 cggcgtcgat ggggtcgcgc gcgtcctcga tttcggcgtg gccaaagccg cgacgcggtc
     2221 gcaggtgacg aaggacggcg aaataaaggg caagatttcg tacatggcgc ccgagcagct
     2281 caaggctcat gcgatcgatc gccgcacgga cgtgttcgcg gcgggcgtcg tgctgtggga
     2341 agcgctgacg gggcggaggc tctttcgcgg cgacgatctc gggcagacgg tcgagaaggt
     2401 gctccacggc gcgattccat caccgagcac gctgcgacga gacgttacga aggcgctcga
     2461 cgcggtcgtg ctgagggcgt tgcagcgcgt tccggcgttc cgcttcgcaa ccgctcgtga
     2521 cttcgcgatc gcactcgaag gcgccactac catcgcttcg gcgagcgagg tcgccgattg
     2581 ggtacgctcg attggcggcg gcaacctcga gcaaaaaatt cagcgcgtcg cggcgatcga
     2641 gagccactcg agcagtcgag tcaaagtggg cggtcggggc ccgctcgata cgccgctcgg
     2701 gctgcgcgcc gtaggt
//
LOCUS       DASTKS010001016         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1053743, whole genome shotgun sequence.
ACCESSION   DASTKS010001016 DASTKS010000000
VERSION     DASTKS010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053743"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VFQ35_21475"
     CDS             complement(<1..1053)
                     /locus_tag="VFQ35_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HET9933296.1"
                     /translation="MSLPNGEFPHLHTIVLAGGSGTRFWPASRRALPKQLLALGKSPD
                     SSLIAETVRRVEPLCAPERVVVATSKRLIEATRRALPWLPSHSFLGEPVARNTAPCIA
                     WATAVIARRDPEAMVMVLPSDHHIGDPARFLGALRTALESAKSAITTIGIEPTRPDTG
                     YGYIERGAPVAEGVYDVARFVEKPNRATAEEYLRGGRYVWNSGMFFFRASELLAAVAR
                     FAPDVWACIERIEAAAKHGAQAEAEATEEAFAAMPSISIDYAVMEKASPLRVVPASFG
                     WSDLGSWESAWDLAEKDELGNSRTDHAVFVDARNNLVVDHSSRARDKVVALVGVENLC
                     IVQTEDALLVIPRDRSQ"
     gene            1250..1852
                     /locus_tag="VFQ35_21480"
     CDS             1250..1852
                     /locus_tag="VFQ35_21480"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET9933297.1"
                     /translation="MAKDPVRCGWCGTDPLYCAYHDTEWGVPSYDDRTLFEFLLLEGA
                     QAGLSWITILRKRDAYRRAFADFDPEKVARFNQRKVEALLRDPGIVRNRLKVEAAIKN
                     ARAFLAVQEERGSFADYQWDFVDGTPVVGNYESLKEIPARTEVSDRFSKDLKRRGFSF
                     VGSTIIYAHMQAVGMVNDHIAACFRQKQVKRAAKRRSAKR"
     gene            1948..>2340
                     /locus_tag="VFQ35_21485"
     CDS             1948..>2340
                     /locus_tag="VFQ35_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933298.1"
                     /translation="MSYRGFGGFRVVLCVLGLLASGCPNPNIYGTPRTLPKGKIAHTI
                     AVEGVGYRFSRRDEYVANGAERTAGAALFVPPSYSLRVGIVDRLDMGLRAANFSSLGL
                     DLKANFLRTEAVDLAVDPFVQWSFSGINT"
BASE COUNT          418 a          746 c          761 g          415 t
ORIGIN      
        1 ctgtgagcgg tcgcggggaa tcacgagcag ggcgtcctcg gtctggacga tgcaaaggtt
       61 ctcgacgccg accagcgcga cgactttgtc gcgagcgcgg ctgctatggt cgacgaccag
      121 gttgttgcga gcgtcgacga acaccgcgtg atccgttcgc gagttgccga gttcgtcctt
      181 ctcggcgaga tcccacgcgc tttcccagga cccgaggtca ctccagccga agctcgctgg
      241 tacgacgcgc agcggggatg ccttttccat gaccgcgtag tcgatgctga tcgacggcat
      301 ggccgcgaag gcctcctccg tggcttccgc ctcggcctgg gcaccgtgtt tggcagcggc
      361 ctcgatgcgt tcgatgcacg cccaaacgtc gggggcgaag cgcgctaccg ccgcgagtag
      421 ctcagaagcg cggaagaaga acattccgct gttccacacg taacgaccgc cacgcaggta
      481 ctcttccgcg gtcgctcggt tcggcttctc gacgaagcga gccacgtcgt agacgccctc
      541 ggccaccggc gcgccgcgct cgatatatcc atagccagtg tcggggcggg tgggctcgat
      601 gccaatcgtg gtgatcgcgc tcttcgcgga ctcgagcgcc gtgcgcagag cgcccaagaa
      661 gcgtgctgga tcgccgatgt ggtgatcgct cggcagcacc atgaccatcg cttccggatc
      721 gcgccgagcg atgaccgcag tggcccaagc gatgcacgga gccgtattgc gcgccaccgg
      781 ctcgcccagg aacgagtgcg agggcagcca cggcaacgcg cgccgagtcg cttcgatgag
      841 gcgcttggac gtcgcgacga cgacgcgctc cggcgcgcag agcggttcga cgcgccgcac
      901 cgtctctgcg atcaaagagg agtcgggtga tttccccaat gcgagcagct gcttcggcaa
      961 ggcgcgacgc gacgcgggcc aaaatcgagt gcccgagccg cccgcgagaa cgatggtatg
     1021 aagatgcgga aattcgccgt tcggcaggct catggccggc gtcatagcgc gtcttttcgt
     1081 ggtttgcatc ggccttggcc gggggctgaa agcgagccgc ccggcgaacg ccccgaggcg
     1141 tccccggagt cactcgcgag agctcgcggg tccgcgccca cgacgtcgcc tcccgcggcg
     1201 tcgcatgaac cgagggtgcc gcagcgctcg aatcgcctat ccttgccgaa tggcaaagga
     1261 cccggttcgc tgcggttggt gcggtactga cccgctctac tgcgcatatc acgacacgga
     1321 gtggggagtt cccagttacg atgaccgcac gctattcgag ttcttgctgc tcgaaggcgc
     1381 acaggcgggg ctcagctgga taacgatcct acgcaaacgc gatgcatacc gccgggcctt
     1441 cgccgacttc gatcccgaga aggtcgcgcg cttcaatcaa cgtaaggtcg aggcgctcct
     1501 gcgcgatccc ggcatcgtgc gaaaccgact caaggtcgaa gccgcgatca agaacgcgcg
     1561 cgccttcctc gccgttcaag aggagcgcgg cagcttcgct gattaccaat gggacttcgt
     1621 ggatggcact cccgtcgtcg gaaactacga gtcgctgaaa gagattccgg cacgcaccga
     1681 ggtatcggat cgcttcagta aagatttgaa gcgacgcggg ttctcgttcg tcggctcgac
     1741 catcatttac gctcacatgc aagccgtagg tatggtcaac gaccacatcg cggcgtgctt
     1801 tcggcaaaaa caggtcaaaa gagcggccaa acgccggtcc gcaaagcggt agccgggcat
     1861 accggaacgc gagcgaggca agcactggtc cggggcggag agcaaactct cgctacgccg
     1921 cgcgggggat cccctatagt agcgcccatg tcttatcgtg ggttcggtgg gttcagggtg
     1981 gtgttgtgcg ttctcggctt gctggcttcg ggttgtccta acccgaacat ttacgggact
     2041 ccgcgcaccc tgcccaaggg gaagatcgcg cataccatcg cggtcgaagg cgtgggatat
     2101 cggttctcgc ggcgagatga atacgtcgcg aacggagcgg agcgcaccgc cggcgccgcg
     2161 ctcttcgtgc caccgagcta ttcgctccgc gtgggcatcg tcgatcgcct cgacatgggc
     2221 ttgcgcgccg cgaacttctc ctcgctcggc ctcgatctga aagccaactt cttgcggacg
     2281 gaggcggtcg atctcgccgt cgatccgttc gtgcagtggt cgttttccgg aatcaacacc
//
LOCUS       DASTKS010001017         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_383426, whole genome shotgun sequence.
ACCESSION   DASTKS010001017 DASTKS010000000
VERSION     DASTKS010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383426"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VFQ35_21490"
     CDS             complement(<1..1209)
                     /locus_tag="VFQ35_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933299.1"
                     /translation="MQILVSALSALCALIASSPARADEQPKSDSNDISEIEAALARDA
                     KPEGDGAASAVPPPTTSPAPSRGGGVQSLNPDISVIGDFAFAAFSRDENHQTGGHDPT
                     ETGFNLQQLELSLSGAVDPYFRFDSYLVFGAEGFELEEAYGTTLDLPGHLQARAGQFL
                     TRFGRLNPTHPHAWDFLDQPFALGRVFGGEGNRGLGAELSWLTPLPWYVELVGSMTRA
                     DGEGSNRSFYGDANPKIASVRDFLYIGALKQFFPLSENWSLAWGLSAARGPNPTGPNH
                     GTYVYGSDVYLKYRPITRQSQTELSLQAEWLVRRRHASDAMLYDVGSYAQVVYRFARR
                     YSAAVRYEYGTPSRDSSGTLRVDTLDPDWVATRTRESVAFTFYPSEFSRLRLQGSRDS
                     GLGDGAAVWAA"
     gene            complement(1328..1759)
                     /locus_tag="VFQ35_21495"
     CDS             complement(1328..1759)
                     /locus_tag="VFQ35_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933300.1"
                     /translation="MRHSRSTVPQSALGRWLVSLLSTLALGLLAAGHVLPVLHFALVA
                     HELCAEHGALHHVEQPSKPGLERARSARPEATNAAANAGHEHDDCNIVATATSRAVLS
                     ERAELAKLPPAVPPSRLSLRARATERSVALLDYAPKLAPPV"
     gene            1845..>3081
                     /locus_tag="VFQ35_21500"
     CDS             1845..>3081
                     /locus_tag="VFQ35_21500"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9933301.1"
                     /translation="MQDPNEVAQGLNEIAALLSFDGAPRFKLKAYETAAEIVKTVGEE
                     LGPLVEQNRLRELQGIGSALSQQIQELWNTGSSELLNRLRAAHPEGAAELVQIEGMTP
                     KRIVALHAALGIRSIDDLRAACLAGRVQKVAGFGAKTEERLLASCERFLNRKEGPPPR
                     VVLSDALAIAEKFERELRHDFAEVHVAGALRRGEETLNELDLFVSGDRERAWERLSSL
                     RQVLRVDRSLGVAHLSGGLLLRLHAAERDLGNAWLAATANAAHWEMLEARARERGFAL
                     SPAAGLGHRGFASEDELYRALDLPYIPPELRVGTNEIQRAERGEFAELVELGDVQGMV
                     HCHTTYSDGRDSIEAMARAAHELGMKYITITDHSPSAHYARGVSVERLKEQWDEIAAA
                     EERVPIRILRGTESDILSDG"
BASE COUNT          533 a          975 c         1077 g          496 t
ORIGIN      
        1 cgcggcccaa accgcggctc cgtctccgag gcccgagtcg cgcgagccct gcaagcgaag
       61 gcgcgagaac tcgctggggt aaaaggtgaa ggcgacgctc tcgcgggtgc gcgtcgcgac
      121 ccaatcaggg tcgagagtgt cgacccgcag cgtgccgctc gagtcccgcg aaggcgtgcc
      181 atattcgtat cgcacggcgg cgctgtaccg tcgcgcgaag cgataaacga cctgagcgta
      241 gctgcccacg tcgtacaaca tcgcgtcgct cgcgtggcgc ctccggacga gccactccgc
      301 ttgcaggctg agctcggttt ggctctgtcg cgtaatgggc cggtacttca ggtacacgtc
      361 gctgccgtag acgtaagtgc cgtgattcgg cccggtcgga ttcggccctc gcgctgccga
      421 gagcccccaa gcgagcgacc agttctccga caaggggaag aactgcttca aggctccgat
      481 gtacaagaag tcgcggacgc tcgcgatttt cgggttggcg tcgccgtaga agctccggtt
      541 cgagccttcg ccgtcggcgc gcgtcatcga accgacgagc tcgacgtacc aaggcagggg
      601 agtgagccag gaaagctcgg cgccgaggcc gcggttgcct tcgccaccga agacgcgacc
      661 gagcgcgaat ggttggtcca ggaaatccca cgcgtgcggg tgtgtgggat tcagacgacc
      721 gaagcgagtg agaaactggc ccgcgcgcgc ctgcaggtga cccggcaagt cgagcgtcgt
      781 tccgtaggct tcctcgagct cgaagccttc ggctccgaac accaggtagc tgtcgaagcg
      841 gaaatacggg tcgaccgccc ccgaaaggga cagctcgagc tgctgtaggt tgaaccccgt
      901 ttcggtcgga tcgtgtccac cggtttggtg attttcatcg cgcgaaaagg ccgcgaacgc
      961 aaaatctccg atcaccgaaa tgtcggggtt caatgactgc acgcccccgc ctcgcgacgg
     1021 agcaggcgag gtcgtcggcg gtgggacggc ggaggcggcg ccatcgccct ctggcttcgc
     1081 gtcgcgggcg agcgcggctt cgatttcgga tatgtcgttg gaatcggatt tgggctgctc
     1141 gtcggcgcgc gccggcgagc tcgcgatgag cgcgcagagg gcgctcaagg cactgactag
     1201 gatttgcatg aatgtaccaa ccccgtttga gtcgactgga tcgcgaagag cgtcgctgcg
     1261 cgcaaacgcg gcgcaacgct ccggcacgag cgtcgcatcg gcgacgcccc actgggagcg
     1321 aaccagatca gacgggcggg gcgagcttcg gagcgtagtc gagcagcgcg acgcttcgct
     1381 cggtcgcgcg ggcgcgcagc gagagccggc tcggagggac ggcgggcggc agcttggcga
     1441 gctcggcacg ctcggagagc accgcgcggc tcgtcgcggt cgcgacgatg ttgcagtcgt
     1501 cgtgctcgtg ccccgcgttg gcggcggcgt tggtcgcttc cgggcgggcg gagcgagcgc
     1561 gctccaggcc gggcttgctc ggctgctcga cgtgatgcag cgcgccgtgc tcggcgcaaa
     1621 gctcgtgcgc gacgagggcg aagtgaagca ctggcagcac gtggccggcg gcgaggagcc
     1681 cgagcgccaa cgtactcaac aagctcacca gccagcgccc gagcgccgac tgcggaaccg
     1741 ttgaacgcga atgcctcacg cgggcgagtt ttgcacgaaa ccggcagcat gccaatgcac
     1801 ggcccggccg tgctcggcgg cggatatcaa agcagacttc cttggtgcag gaccccaacg
     1861 aagtcgcgca gggcctcaac gagatcgcgg cgctcttgag cttcgacggc gcgcctcgtt
     1921 tcaagctgaa ggcctacgaa acggccgccg aaatcgtgaa aaccgtgggt gaggaactcg
     1981 gtcccttggt cgagcagaac cggcttcgag agctccaggg catcggttcc gcgttgtcgc
     2041 agcagattca agagctctgg aacacaggct cgtcggagct tctcaatcgt ttgcgtgccg
     2101 cgcacccgga gggcgccgcc gagctcgtgc agatcgaagg gatgacgccg aagcgcatcg
     2161 tggctttgca cgccgcgcta ggcattcgct cgatcgacga cctgcgcgcg gcgtgtctcg
     2221 cggggcgggt ccagaaagtc gcggggttcg gtgccaagac cgaggagcgc ctgctcgcgt
     2281 cgtgtgaacg gttcttgaat cgcaaggaag gtccgccccc gcgcgtcgtg ctctcggacg
     2341 cgctcgcgat tgccgaaaag ttcgagcgcg agctgcgcca cgacttcgcc gaagttcacg
     2401 tcgctggcgc tctgcggcgc ggggaagaga ctctcaacga gctcgacctg ttcgtctcgg
     2461 gagatcgcga aagggcctgg gagcggttgt cgagcttgcg tcaggtgctt cgcgtggacc
     2521 ggtcgctcgg cgtcgcgcac ctcagcggtg gacttttgct gcgccttcac gccgccgagc
     2581 gcgatctcgg taacgcctgg ctcgccgcga ccgcgaacgc ggcgcattgg gagatgctcg
     2641 aggcgcgcgc ccgtgagcgc gggtttgcgc tgtcgccagc ggctggtctt ggtcatcgcg
     2701 gcttcgcgag cgaggacgag ctctaccgcg cgctcgatct gccctacatc cctcccgaac
     2761 ttcgcgtcgg cacgaacgaa atccaacgtg ctgagcgcgg cgaattcgcg gagctcgtcg
     2821 agctcggtga cgtgcagggc atggtgcact gccacaccac ctactcggac gggcgcgaca
     2881 gcatcgaagc gatggcgcgc gcggcccacg agcttggcat gaagtacatc acgatcacgg
     2941 atcactcccc gagcgctcac tatgcgcgcg gcgtcagcgt cgaacgactg aaagagcaat
     3001 gggacgagat cgcggcggca gaggagcgcg tgccgatacg tatcctgcgc ggtaccgagt
     3061 ccgacatctt gagcgacggc t
//
LOCUS       DASTKS010001018         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1054063, whole genome shotgun sequence.
ACCESSION   DASTKS010001018 DASTKS010000000
VERSION     DASTKS010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054063"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFQ35_21505"
     CDS             <1..517
                     /locus_tag="VFQ35_21505"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933302.1"
                     /translation="LKRLLKEKGLHQAVMFHDVRWGAEHDGRFLWVLLNSGSCGAYAF
                     NHDPHTLKGVHSYRQPSLYFPTPGGTFAGESLPGKITWARAYIKNDELWMDVGRGEVV
                     KLPPEKRDAWWEGTTRQWPFMAADLGVGCETLMAHYQSNHVAVAYGDVFGEMVALSAE
                     LGFRVRVLGDS"
     gene            514..2142
                     /locus_tag="VFQ35_21510"
     CDS             514..2142
                     /locus_tag="VFQ35_21510"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9933303.1"
                     /translation="MSVQAFLGVDVGTASVRAGLFDAEGKRLGSGVCPIQIFRPAEDF
                     VEQSSTDIWRAVCTAVRAALHEAAMAPTNVLGIGFDATCSLVVLDADGKPLSVSPSGN
                     PEQNVIVWMDHRAIDDAAAINRTSHDVLRYVGGVISPEMEMPKLRWLKREKPDTWQKA
                     ALFLDLPDFLSYRATGSRTRSLCTTVCKWTYVGHEQRWAVDFLAQIGLEDLTRDAFSR
                     IGNEVRPMGERLGGLTGEAAAELGLIAGTPVAVGIIDAHAGGLGLIGASLDGQAPTEA
                     ELERRLALIGGTSSCHMAVSREARFVPGVWGPYYSAMVPGLWLTEGGQSATGALIDHV
                     IHSHARGRELTEQAQREDTSVYELLNRALARLSPQTAPELTAELHVFPDFHGNRSPRA
                     DPTLRGMLSGLRLSDSVESLALVYFATIQAIAHGTRHILAALAAQGYRIDTLLACGGD
                     TKNALFLREHADICGCRVVLPREPEAVLLGASLLGAVAAGRYASVVEAMGAMSAAGRV
                     VEARTGAVAAYHEKKHRVFLRMYEDQMAYRALMS"
     gene            2156..3448
                     /locus_tag="VFQ35_21515"
     CDS             2156..3448
                     /locus_tag="VFQ35_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9933304.1"
                     /translation="MGGTPAAYAIDFGTTNSLIAAADGERTWEPLAIDAGSSDPTVLR
                     SILFFPEDKSAVSCGSEALRDAAECGMRGRLIRSIKRFLPAATFTETRIGARRYALEE
                     LVAIVIRTLRERANRALNADVRAVVIGRPARFSDDLEADALAAERLQNAARIAGFEQV
                     TLCEEPVAAALDCASEADKRELVAVCDLGGGTSDFTVARLGAGKSDVLAVGGVALAGD
                     ALDGALMREHVSPYFGSRVSYKMPFGENVLTFPKPLLEKMCSPAELALLDRRDVLEFL
                     RTVKSFALTEDDRERMERLLCLVEDRLGFSLFEAIDRLKRELSTGDEGKFLFDYPGIE
                     IAQSVTRTGFERSAQSPIERILARFEETLAAAGVSASDLDRVYLTGGTAQVPALIDAI
                     ADRAGAHKLSHVRTFHAVIQGLAERARELVQTGQFAAS"
     gene            complement(3474..>4081)
                     /locus_tag="VFQ35_21520"
     CDS             complement(3474..>4081)
                     /locus_tag="VFQ35_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933305.1"
                     /translation="GLRITPFIVTLGTMSLLRGAAKGLADEQKIDADPRGIDAWMTAP
                     ANGWFPPGVWVAIIIALIAAVLLRSSVFGRHVVAMGSNEHTARLCGVPIHRVRILVYT
                     LAGALAGIAGILEFATLTVGDPTDSIGLELEVIAAVVIGGASLAGGQGSIVGAMVGAL
                     LMTEIKTGCTHIGLPNWVQEILTGAIIVVAVALDRVRKRRD"
BASE COUNT          672 a         1299 c         1402 g          708 t
ORIGIN      
        1 gctgaagcgc ctcttgaaag agaaggggct gcatcaagcg gtgatgttcc acgacgtgcg
       61 ttggggcgcc gagcacgacg ggcgcttctt gtgggtgctc ctcaactctg gttcgtgcgg
      121 cgcgtacgcc ttcaaccacg accctcacac gttgaaggga gtgcacagct accgccagcc
      181 gtcgctctat ttcccgacgc ccggtggaac cttcgctggc gagagtttgc ccggcaaaat
      241 cacctgggcg cgtgcctaca tcaagaacga cgagctctgg atggacgtcg ggcgcggtga
      301 agtcgtcaag ttgccgcccg aaaagcgcga cgcttggtgg gagggtacga cccggcaatg
      361 gcccttcatg gccgcggatt tgggcgtcgg ctgcgagacc ttgatggccc actaccaatc
      421 gaaccacgtg gctgtcgcct acggcgacgt attcggcgaa atggttgcgc tcagcgccga
      481 gctcgggttc cgcgtgcgcg tgctgggcga ctcatgagcg tgcaggcgtt cctcggggtg
      541 gacgttggta cggcgagcgt ccgcgccggg ctcttcgacg ccgaaggtaa gcgcctcggc
      601 tcgggcgttt gcccgatcca aatctttcgg cccgccgagg acttcgtcga acagtcgtcc
      661 acggacatct ggagagccgt atgcaccgcg gtacgcgctg cgttgcacga ggcggcgatg
      721 gctcccacga acgtgctcgg catcggcttc gatgcgacct gctctttggt cgtgctcgac
      781 gccgacggca agccgctcag cgtgagtccg agcggaaacc cggagcaaaa cgtcatcgtc
      841 tggatggatc atcgcgccat cgacgatgcg gccgcgatca atcgcacgtc acacgacgtg
      901 cttcgttacg tcggcggagt catctcgccg gagatggaga tgccgaagct ccgttggctc
      961 aagcgcgaga agccggacac ctggcaaaag gccgcgctgt tcttggactt gccggatttc
     1021 ctgagctatc gagcgacggg ctctcgcacg cgttcgctgt gtacgacggt gtgcaagtgg
     1081 acttacgtcg ggcacgagca gcgctgggcc gtggatttcc tcgcgcaaat cggcctcgaa
     1141 gacctgactc gcgacgcgtt ctcacgcatt ggcaacgaag tacggccgat gggtgagcgc
     1201 ttggggggtc taaccgggga agccgcggcc gagcttgggc tcatcgcggg cacgcccgtc
     1261 gcggtcggca tcatcgacgc tcacgcgggc ggcctcggcc taatcggtgc ttcgctcgac
     1321 gggcaagcgc cgacggaagc agagctcgag cgtcggctcg ctttgattgg cggcacttcg
     1381 agctgccaca tggcggtgtc acgtgaggcg cgcttcgttc cgggcgtgtg gggaccttac
     1441 tattcggcga tggttccagg gctctggctc accgaggggg gacaatcggc gacgggggcg
     1501 ctcatcgatc acgtgattca ttcccacgcc cgtgggcgcg agctcacgga gcaggcgcag
     1561 cgcgaggaca cgagcgtgta cgagctcttg aaccgcgcgc ttgcacggct gtctccgcaa
     1621 acggcgcccg agctcaccgc cgagctccac gtctttcccg attttcatgg caaccgttcg
     1681 ccgcgcgcag atcctaccct gcggggcatg ctgagcggct tgcgcctgtc cgacagcgtc
     1741 gaatcgctcg ctctcgtgta cttcgcgacg attcaagcca tcgcgcatgg gactcgtcac
     1801 attttggccg cgctcgcggc gcagggttat cgcatcgaca cgctgctcgc atgcggtggt
     1861 gacacgaaga acgcgctctt cttgcgcgaa cacgccgaca tttgcggttg ccgggtcgtg
     1921 ctcccgcggg aacccgaggc ggttttgcta ggcgcttcac tgcttggagc ggtcgcggcc
     1981 gggcgctacg cgagcgtggt ggaagccatg ggagcgatga gcgcggccgg acgcgtggtc
     2041 gaggcgcgca cgggggccgt ggctgcctac cacgaaaaga agcatcgagt gtttttgcgc
     2101 atgtacgaag atcagatggc gtaccgcgcc ctcatgagtt agcccactcg agattatggg
     2161 tggcactccg gcggcctacg ccattgactt cggaacgacc aattcgctga tcgcggcggc
     2221 ggacggcgag cgcacctggg agccgctcgc catcgacgcg ggcagcagtg atcccacggt
     2281 gctccgcagc atcttgttct tccccgagga caagagcgcg gtttcatgcg gtagcgaggc
     2341 gctgcgcgac gccgccgaat gcggcatgcg aggccgtttg atccgctcga tcaagcgctt
     2401 cttgcccgcc gccaccttca cggagacccg gatcggggca cgccgctacg cgctcgaaga
     2461 gctcgtcgcg atcgtaatcc gaaccctgcg agagcgcgcc aatcgagcgt tgaacgccga
     2521 cgtgcgggcg gtcgtgattg gcaggccggc gcgcttctcg gacgacttgg aggcggacgc
     2581 gctcgcggcc gagcgcctgc agaacgcggc gcgcatcgcc gggttcgagc aggtcacgtt
     2641 gtgcgaagag ccggtcgcgg cggcgctcga ctgcgcaagc gaagcggaca agcgcgagct
     2701 cgtcgccgtc tgtgatcttg gcggcggcac ctccgatttc acggtggcgc gcctcggcgc
     2761 cgggaagagc gacgtgctcg cggtgggtgg cgtcgcgctg gccggcgacg cgctcgacgg
     2821 cgcgctgatg cgcgagcacg tctcgccata ctttggctcg cgagtctctt acaagatgcc
     2881 gttcggcgag aacgtgctca cgtttccgaa gccgttgctc gagaagatgt gctcacccgc
     2941 cgagctcgcg ctgctcgacc gccgcgacgt gctcgagttt ttgcgcaccg tgaagagctt
     3001 tgcgctcacc gaagacgacc gcgagcgcat ggagcgcctg ctctgcctcg tcgaagaccg
     3061 gcttggtttc tcgctgttcg aggcgatcga ccgcttgaag cgagagctct cgaccggcga
     3121 cgagggaaag tttctgttcg actacccggg gatcgaaatc gcgcagtcgg tcacgcggac
     3181 gggcttcgag cgctcggcgc aatcgcccat cgagcgcatc ctggcacgct tcgaagagac
     3241 gctggctgcg gccggcgtga gcgcgagcga cctcgatcgc gtctacctca cgggcggtac
     3301 ggcgcaggtg ccggcgctga tcgatgcgat tgcggatcgc gccggggctc acaagctgtc
     3361 gcacgtgcgg acgtttcacg ccgtgatcca gggcctcgcc gagcgcgctc gcgagctcgt
     3421 gcaaacgggc cagttcgcgg cgagctgagg cccgggctgg tccgcgaaga gcgtcaatcg
     3481 cggcgttttc gcacgcgatc gagcgcgacg gcgacgacga tgatcgcgcc ggtcagaatc
     3541 tcctgcaccc agttcggcaa gccgatgtgc gtgcagccgg tcttgatctc ggtcatcaag
     3601 agcgcgccga ccatggcccc gacaatcgag ccctgaccgc ccgcgagtga agcgccgccg
     3661 atcaccaccg cggcgatgac ttcgagctcg agcccgatcg agtcggtggg gtcgcccacg
     3721 gtgagcgtag cgaactcgag gatcccggcg attcctgcga gcgcaccggc gagcgtgtag
     3781 accaaaatgc gcacgcgatg gatggggacg ccgcagaggc gcgccgtgtg ctcgttcgaa
     3841 cccatcgcga ccacgtgccg cccgaagacg ctcgagcgaa gcagcaccgc ggcgatcagc
     3901 gcgatgatga tggcgaccca aacgccgggc ggaaaccagc cgttcgccgg cgccgtcatc
     3961 caggcgtcga tgccgcgcgg atcggcgtcg attttttgct cgtcagcgag gccttttgcc
     4021 gcgccgcgca agaggctcat ggtgccgagc gtgacgatga acggagtgat gcgaaggccg
     4081 g
//
LOCUS       DASTKS010001019         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1054584, whole genome shotgun sequence.
ACCESSION   DASTKS010001019 DASTKS010000000
VERSION     DASTKS010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054584"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFQ35_21525"
     CDS             <1..366
                     /locus_tag="VFQ35_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933306.1"
                     /translation="PSEQVAEPTTSVLESPKVAPSARSSRSESSSKADSVAAELGVID
                     SVRAALSRGDTSGALSRLDGYAKTYPRGRLALEAEVLRIDALARSGQAAAASKRAEAF
                     LRRFPNSVLASRVRGYLKQ"
     gene            600..1823
                     /locus_tag="VFQ35_21530"
     CDS             600..1823
                     /locus_tag="VFQ35_21530"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9933307.1"
                     /translation="MALITTLLASLAAACGSTTDSLGDTRQPTQVLEPIKGPTTYPNL
                     FKDVLLKSDKEISTKLDTAFDKLFHGDPLTESFYVADGEAAMILDSYHGDQRTEGYGL
                     AMLIAVEMNKQDEFDRIWRGAQAFRYDVGPYEGYFWSVCDRPSGRELCVDPYGLQQFT
                     MALLLANTRWKDSPSRVDYAKDASDLLYLLLHKEDMNGGVVDGVTNTFDEDSKLIPYQ
                     PNRAGVNITRPSLAMPAYYALWYQATTNPFFLEAAEKARDYLRAVSHPKTGLTPVRSD
                     FDANPLPGFDTFYADCYRVQLNLMLDYVWSEQPVPSGDLSDRLLTFFTTQGPNYGRNF
                     SLDGTVVLDTGHDAALVAANGMSAIAAPNNPQRLGFVQAVWSQPLPTGTPRYYAGLMQ
                     LLAMLALSGQLRADW"
     gene            1894..3114
                     /locus_tag="VFQ35_21535"
     CDS             1894..3114
                     /locus_tag="VFQ35_21535"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933308.1"
                     /translation="MREFLLTRMDANGDCWGDDAEGSPFRASGPVPGDAVALIGVAQR
                     TQVRRAYELVRGAPERRAAPCPVQRQCGGCRLMPLNEAAQQRVKLEILRAELERRGIC
                     APRLDWHPASSSLAYRNRIRLRLAAGKLSFFNQDKDEGCVVLAPTLQSALERLRGLEG
                     ADCALCSFSHCEVRAHDLDGRPALALSGRTDPGLLRAELAELQRLLPEFLLGVTGDPA
                     IPAQRRLLGALEMFVPLDAFLQVNDAVNERLIAWVVNSVRALRVGNVLDLFAGAGNFS
                     LPLAAAGSAVTAVERHGPCITALGRSAARAGVACEGVVARADEAVSALVAAKRRFDCV
                     VVDAPRAGAGALLARFDDLGVRFVVLCSCYAPSFARDLAALLEQGFRIRAITIFDMFP
                     QTHHVECVAVLAKE"
     gene            complement(3117..>4340)
                     /locus_tag="VFQ35_21540"
     CDS             complement(3117..>4340)
                     /locus_tag="VFQ35_21540"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9933309.1"
                     /translation="HSQQPLDTSWQSVALATPLVTENAFDAFFKFPSAGTVILGSGYP
                     DASLQPATLLEKAAQRAAKQSRTWARLPVEGLPELREWFARDLGGAARADQVLLVPGG
                     QAALSTLFRALVPFGGPLVVESPSYFGALGIARAFGLRVVPVPHDDEGIRPDLLRAAL
                     ASTGARVLYLQPAFSNPTGITLSESRRKEVLEIVRSANAFLIEDDYARDLVDGAPPVP
                     LYREGDGHVIYVRSLTKSTAPGVRVGAIVAEGPVLGRLKTARAIDDWFLSGLLQATAL
                     ELVSSPSFPGYVQKLVHTLRERRSRTVDTLRKHFPEAHLRRVPSGGFSLWLELPQGTD
                     ESAFVQAALEAGVQINPGAPWFAAEPSGPHLRLSIAGASAELLEEGVRRLGALFQRLQ
                     ASSDLARDKPGRRRA"
BASE COUNT          720 a         1444 c         1407 g          769 t
ORIGIN      
        1 ccaagcgagc aggtcgccga gccaacaacc agcgtgctcg agtcgccgaa ggtggctccc
       61 tcggcgcgct cttccaggtc ggagtcgagc tccaaagccg attcggtcgc ggctgaactc
      121 ggggtgatcg acagcgtgcg cgctgcgctg agtcggggcg acacgagtgg ggcgctctcg
      181 cggctcgatg gctacgctaa gacctatcct cggggtcggc tcgctctcga agccgaggtg
      241 ctgcgcatcg acgcgctcgc tcgctcgggt caggcggccg ctgcctcgaa gcgcgccgaa
      301 gcattcttgc ggcggttccc gaacagcgtg cttgcgtcgc gtgtccgggg atatctgaag
      361 cagtagtcag cagcgcgtcc ggcacggaaa tttctcgtag agattttccg ctgttgccag
      421 gccgacgtcg cttcgcgctc gcggtgcagg tcgacacggc gaaagtcggc gcgttggcct
      481 cgtccaatca ccgactcgtc gctacactcg tccgcgtgag tgccgggcgg cctcggtttc
      541 gagctcctga tcaatcgttg cttccagatc ggaggcggcg tgcggcagcg ccacgagtca
      601 tggcgctcat cacgactctg ctcgcctcgc tcgcggccgc gtgcggcagc accacggatt
      661 cgctcggcga cactcgtcag ccaactcagg tactcgaacc aatcaaaggc ccgaccacgt
      721 atccgaacct gttcaaagat gtgctgctca agagcgacaa agagatctct acgaagctag
      781 atacggcctt cgataaattg ttccacggcg acccgctcac cgagtcgttc tatgtggccg
      841 atggcgaagc cgcgatgatc ctcgactcgt atcacggcga ccagcgcacc gagggatacg
      901 gccttgcgat gttgatcgcg gtcgagatga acaagcagga cgagttcgat cgcatttggc
      961 gcggagccca ggcgtttcgc tacgacgtgg gtccttacga gggctacttc tggtcggttt
     1021 gcgataggcc ttccgggcgc gagttgtgcg tcgaccctta cggcttgcag caattcacca
     1081 tggcgttgct gctcgcgaat actcgctgga aagactctcc gtctcgcgtc gactacgcga
     1141 aggacgcaag cgacttactc tacctcttgc tccacaaaga ggacatgaac ggtggcgtcg
     1201 tcgacggcgt gacgaacacc ttcgacgaag attccaagct catcccttat cagccgaacc
     1261 gcgccggcgt gaacatcacc cgcccttcgc tcgccatgcc cgcgtactac gcgctctggt
     1321 atcaggcgac cacgaaccca ttctttctcg aagcggcaga gaaggcgcgc gattatctgc
     1381 gcgcggtgtc gcacccaaag actggcctca cgccagtgcg ctctgacttc gacgccaatc
     1441 ccctcccagg gttcgatacc ttctacgccg actgctaccg cgtgcagctg aacctcatgc
     1501 tcgactacgt gtggagcgaa cagccggttc cgtccggtga cttgagtgat cgtctgctca
     1561 cgttcttcac cacccaaggg ccgaactacg gtcggaattt ctcgctggac ggcacggtgg
     1621 tcctcgatac ggggcacgac gcggccttgg tcgcggcaaa cggcatgagc gccatcgcgg
     1681 cgcccaacaa tccccagcgc ctcggcttcg ttcaggccgt gtggagtcag ccgttaccga
     1741 cggggactcc tcgctactac gcgggcctga tgcagctctt ggcgatgctg gctctgagcg
     1801 gtcagctccg agcggattgg tgaggctccc gtacctcgca cgtcgcatgc ccggacgctc
     1861 gagctcgcgc ggcccttcgt cgaggcatca cgagtgcgcg agttcttact gactcgcatg
     1921 gatgcgaacg gcgattgttg gggggatgac gccgaaggct cgccgtttcg cgcgagcggc
     1981 cctgtccccg gtgatgcggt cgcgctgatc ggcgtcgcac aacgcacgca agttcggcgt
     2041 gcctacgagc tcgtccgcgg cgcgccggag cgccgagccg cgccgtgccc ggtgcagcgg
     2101 caatgcggtg ggtgccggct gatgcccttg aacgaggccg ctcagcaacg cgtgaagctc
     2161 gagatcttgc gcgccgagct cgagcgacgt gggatctgcg cgccgcggct ggattggcac
     2221 cctgcttcgt cctcgctcgc ttaccgaaac cgcattcgcc ttcgcctcgc cgccggtaag
     2281 ctgagcttct tcaaccagga caaggacgag ggctgcgtcg tgctcgcgcc gactctgcag
     2341 agcgcgctcg agcggcttcg cgggctcgaa ggagcagatt gcgctctctg ctccttcagc
     2401 cattgcgaag ttcgcgctca tgatctcgac ggaagacctg cgctcgcgct cagcggtcga
     2461 acggacccgg gtttgctccg cgccgagctc gccgagctcc agcggctgct accggaattc
     2521 ttgctaggcg taaccggcga tcctgccatc ccggcgcagc gccgactcct cggcgcgctc
     2581 gagatgttcg tgccgctcga cgcgtttctg caggtcaacg acgccgtgaa cgagcgcctc
     2641 atcgcgtggg tggtgaactc ggtgcgagcg cttcgcgtcg gcaacgtgct cgacctattt
     2701 gcgggcgccg gcaacttcag tttgccgctc gcggccgcgg gctcggcggt gaccgcggta
     2761 gagcgtcacg gcccctgcat caccgcgctc ggcaggtcgg cggcccgcgc cggtgtcgca
     2821 tgcgaaggag tcgtggctcg cgccgacgag gcggtttcag cgctcgtcgc ggcgaagcgt
     2881 cgcttcgact gcgtcgtcgt ggacgctcct cgcgccggcg ccggggcgct gctcgcgagg
     2941 ttcgacgatc tcggcgtgcg cttcgtggtg ctctgctcgt gctacgcgcc gagcttcgcc
     3001 cgcgatctcg cggcgctgct cgagcagggc tttcgcattc gagcgatcac gatcttcgac
     3061 atgtttcccc agacgcacca tgtagagtgc gtcgccgtgc tcgcaaaaga atgagctcaa
     3121 gcgcgccgcc gaccgggctt gtctctggcc aggtcggagc tcgcctggag ccgctgaaat
     3181 agcgctccga gccggcgaac gccttcttcc aagagctcgg cgctggcgcc cgcgatgctg
     3241 agtcgcagat gggggccgct cggctccgcg gcgaaccacg gtgccccggg gttgatctga
     3301 acgcctgcct cgagcgcggc ttgcacgaac gcgctctcgt cggtgccttg cggcagctcg
     3361 agccacagcg aaaacccacc gctcgggacg cgtcgcaggt gagcctccgg aaaatgcttt
     3421 cgcaaggtgt cgacggtgcg agagcgccgc tcgcgcagag tgtgcacgag cttctgcacg
     3481 tagccaggga agctcggcga gctcacgagc tcgagggccg tcgcttgcaa gagccccgac
     3541 aaaaaccagt cgtcgatcgc gcgcgccgtc ttcaagcgcc cgagcacggg gccctcggct
     3601 acgatcgcgc cgacgcgaac tccgggcgcg gtcgacttgg tcaacgagcg cacgtaaatg
     3661 acgtgcccat ccccctcacg atagagcggc accggcggcg ccccgtcgac caagtcgcgt
     3721 gcgtagtcgt cctcgatgag gaaggcgttc gcgctccgga cgatctcgag cacttcctta
     3781 cgacgtgatt cggacagcgt gatgccggtc gggttcgaaa aggcgggctg taagtagaga
     3841 acgcgcgctc ccgtcgaggc gagcgcggcg cgcagcaaat ccggccgaat tccttcgtcg
     3901 tcgtgcggga ctggcaccac ccgcagcccg aacgcccgcg cgatcccgag cgcgccgaag
     3961 tacgacggcg actcgacgac gagcggacct ccaaagggca ccaaggcgcg gaacaacgtc
     4021 gatagcgcgg cttggccacc gggaacgagc agcacttgat cagcgcgagc ggctccacca
     4081 aggtctcgag caaaccactc gcgaagctcg ggtagcccct cgacgggcag gcgtgcccag
     4141 gtgcgagatt gcttcgcggc tcgttgagct gctttctcga gtagggtcgc cggctgcagc
     4201 gaggcatcgg ggtagccact tcccaggatc accgtgcccg cggacgggaa cttgaagaac
     4261 gcgtcgaaag cgttctccgt cacgagcgga gtcgcgagcg ccaccgactg ccacgacgta
     4321 tcgagtggct gctgagagtg
//
LOCUS       DASTKS010001020         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_830968, whole genome shotgun sequence.
ACCESSION   DASTKS010001020 DASTKS010000000
VERSION     DASTKS010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830968"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFQ35_21545"
     CDS             <1..564
                     /locus_tag="VFQ35_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933310.1"
                     /translation="DRALGADRRVVVDMPAQSHKPLRRWMDATDVLSYAREMDVRLVL
                     WHVTDGGFDSVAHLEKLLEDFGGSVDFVVVKNEGRSKDFSQLEASPAYERLLALGGRV
                     VALPELDSAVMYKIDRFGSSFWAAVNATEGERALSPLERRRAKLWLERAKTALEAADG
                     SRSQASHSFPRVNAAEQNTLRSDQSVN"
     gene            complement(743..1282)
                     /locus_tag="VFQ35_21550"
     CDS             complement(743..1282)
                     /locus_tag="VFQ35_21550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9933311.1"
                     /translation="MLLLACAPPPSALDARSALSLRGSDARVHDVRKELAEARFTVFV
                     FAAKSCACLDAHAPRLRALHDEYSQKGVAFRLVDSEVGASPSRAMQLARRSDLGFPAL
                     ADPGARLADALGAAFASHTVIVDEGGRIRYSGGIDSDAARLHDDAKLYVRDALDDLLA
                     GNPPRQARSEALGCSLELR"
     gene            complement(1338..2192)
                     /locus_tag="VFQ35_21555"
     CDS             complement(1338..2192)
                     /locus_tag="VFQ35_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET9933312.1"
                     /translation="MAWGQACCAGAAAVTPGRLLLHEPALIGVRVRAALGLGSYGSDG
                     RFRGNPDGTRELDFEQDLFGTLRVLKRGQVGVLLPVLEASRATRSTGSEFGGGLGDVA
                     VSARYDFVWAGENHVVPGIAAFAGASFPSGRAPEDAHLALASDATGVGAVRSSFGLAL
                     EQVFGPVLVQASALVAKRANRTVHGLDSARALEWNTTLAVGYSFPSDVGLAAALSYSW
                     EGRSTLDGENIADSEQRLLEASAAISVPLGQFWRAQGALFLTPPISGVGQNRLTEAGA
                     SGTIIRSF"
     gene            complement(2254..2670)
                     /locus_tag="VFQ35_21560"
     CDS             complement(2254..2670)
                     /locus_tag="VFQ35_21560"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET9933313.1"
                     /translation="MRFGRYTFNELWLGLALLGGVACSSGAVEPPPTVVASESGALRV
                     ELSSPDGIARGLNRFQFEVLEAADESPATELEFRMQPFMPAMGHGSGGEPEVRSIEAG
                     RYEFSSVLLNMAGRWQLRTTISGARSDYVVFDVDVP"
     gene            complement(2868..>3465)
                     /locus_tag="VFQ35_21565"
     CDS             complement(2868..>3465)
                     /locus_tag="VFQ35_21565"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9933314.1"
                     /translation="GRKERVQNPNYSSGSSDWKTSRQYAEYTSSSLNTSGLQSWQYGR
                     FEMRGRIPTSAGMWPAWWTLGVSGEWPSNGEIDIMEFYNGKLLANVACGTSTRWQARW
                     DSATKQLSALGSGWSSEFHVWRMDWDDQNIVLYVDDQQMNTTALSSMLNPNGESPFKQ
                     KAYMLVNLAIGGNNGGDPSNTTFPQRYEVDYIRVFQKM"
BASE COUNT          613 a         1174 c         1107 g          571 t
ORIGIN      
        1 gatcgcgcgc tcggggcgga ccggcgcgtc gtcgtcgaca tgccggcgca aagccacaag
       61 cccctacgcc gctggatgga cgcgaccgac gtcctctcct acgcgcgcga aatggatgtg
      121 cgactggtgc tctggcacgt gacggacggc ggcttcgact cggtcgccca cctcgagaag
      181 ctgctggaag atttcggcgg ctcggtggac ttcgtggtcg tcaagaacga gggccgctcc
      241 aaggatttca gccagctcga ggcctctccc gcttacgagc gactgctcgc actgggcggt
      301 cgtgtcgtgg cgctgcccga gctcgactcg gccgtcatgt acaagatcga ccgcttcggc
      361 tcgagcttct gggctgcggt caatgcgacc gagggagagc gggcgctgtc gccgctcgag
      421 cggcgccgcg ccaagctgtg gctcgaacgt gcgaagacag cgctcgaagc cgccgacggc
      481 tcgcgctcgc aggcgagcca ttcgtttccg cgcgtcaacg cggccgagca gaacacgttg
      541 cggagcgacc aaagcgtcaa ctgagcgcgg cccagggcca agcggtcaca cgagctcgga
      601 catttcggag gagggcgcga tggcttcgta ccagcgccct cccgggctca ggtggccggc
      661 gccaccagta gattcggcgc gttcgccacg tcttcgttgt cccatggcgg cgttggtccc
      721 gttccgccgt gaaatttgtg cctcaacgca gctcgagcga gcagcccagc gcctctgagc
      781 gagcttggcg gggagggttg cctgcgagca ggtcgtcgag cgcgtcgcgg acgtagagct
      841 tcgcgtcgtc gtgcaagcgc gccgcgtcgg agtcgatgcc gcccgaataa cgaattcgcc
      901 cgccttcgtc cacgatgacc gtgtggctcg cgaatgcggc gccaagcgcg tccgccaagc
      961 gggcaccggg gtcggcgagg gccggaaaac ccaagtcgct acggcgggcc aactgcatcg
     1021 cccgcgaagg gctcgcgccc acttcggagt cgactaggcg aaatgcgacc cccttctggg
     1081 aatattcgtc gtggagcgct cgcaaacgcg gggcgtgcgc gtcgagacaa gcacaagact
     1141 ttgccgcgaa cacgaacacc gtgaaccgcg cttccgcgag ctccttccgc acgtcgtgaa
     1201 cgcgtgcgtc actgccgcgg agactgagcg cggaccgagc gtcgagcgcc gaaggcggcg
     1261 gcgcgcaggc aagcaggagc atcgtcagag aggcaacgct ccgggtgatt gtcccaaatt
     1321 cgctcacgct tcgcgcctca aaaactccgg atgatggtgc cgctcgcgcc cgcctccgtg
     1381 agacgattct gtccgacgcc gctgatgggc ggtgtgagga agagcgcgcc ctgcgcgcgc
     1441 cagaactgtc caagcggcac gctgatcgcc gcagaggctt cgagcaaacg ttgttcggaa
     1501 tcggcgatat tttcgccgtc gagagtcgag cgtccttccc aagagtaact gagcgcggcc
     1561 gcgagaccga cgtcgctcgg gaacgagtag ccaaccgcca acgtcgtgtt ccactcgagc
     1621 gcccgagcgc tgtcgagccc atgcaccgtg cgattcgcgc gcttggccac gagcgcgctg
     1681 gcctggacga gcaccggtcc gaacacttgc tcgagcgcca agccgaagct cgagcgcacc
     1741 gcgccgacac ccgttgcgtc cgacgcaagc gccaagtgcg catcctctgg ggcgcgcccg
     1801 cttgggaacg aagcgcccgc gaacgccgcg attccgggca cgacgtggtt ctctccggcc
     1861 caaacgaaat cgtagcgagc gctcacggcg acgtcaccca ggccgccgcc gaactcggag
     1921 cccgtactcc gagtcgcgcg tgaggcctcg agaacgggca gcagaacccc cacttggccg
     1981 cgcttcaaaa cccgcagcgt accgaaaagg tcttgctcga agtcgagctc gcgcgtgccg
     2041 tctgggtttc cgcggaagcg cccatctgaa ccgtacgagc ccagaccgag cgccgctcgt
     2101 acgcgcacgc cgatcaacgc cggctcgtgc agcagcaagc gacccggcgt gacggcggcg
     2161 gccccggcgc agcacgcttg cccccatgcc actcgcgagg ccaacgaagc gagcacgagc
     2221 gcgagcgcac cgagcgccgt tctgaaccac cgactacggg acgtcgacgt cgaagaccac
     2281 atagtcggat cgcgcgccag aaatcgtcgt cctcagttgc caacgaccag ccatgttcaa
     2341 gaggaccgac gagaactcgt accgccccgc ttcgatgctt cgaacttccg gctcgccgcc
     2401 actgccatgc cccatcgcgg gcatgaacgg ctgcatgcga aactcgagct cggtcgctgg
     2461 cgactcgtcc gcggcctcga gcacctcgaa ctgaaatcga ttcaggcccc gcgcgatccc
     2521 gtctgggctc gacagctcga cgcggagagc gccgctctca ctcgcgacta ccgtcggcgg
     2581 gggctcgacg gcaccgctcg aacaagcgac gccaccgagc agggcgagcc ccagccatag
     2641 ttcattgaaa gtgtaccttc cgaagcgcac gagcagctcc tcttgcgaga actctgccag
     2701 ctgagtggca cggttctcac cactctcaaa tgcgtgaaat tttcgcaccc cccgccgcta
     2761 ttgcatcgat cctcgatgca aattttgcat tttggcgcca aacacgcgcg ttcggcttgg
     2821 tgcgttcggg cgccggcgtc gcagccggcg agagagccgc gccggcgtca catcttttga
     2881 aagacgcgga tgtagtcgac ctcgtagcgc tgcggaaagg tggtattcga gggatcgccg
     2941 ccgttgttcc caccgatcgc caggttgacg agcatgtaag ccttctgctt gaacgggctc
     3001 tcaccgttgg ggttgagcat gctcgagagg gcagtcgtgt tcatctgctg gtcgtcgacg
     3061 taaaggacga tgttttggtc gtcccagtcc atgcgccaaa cgtggaactc cgaggaccac
     3121 ccgctgccga gcgccgatag ctgcttggtc gcagagtccc acctggcttg ccatcgggtc
     3181 gacgtgccgc acgcgacgtt ggcgagcagc ttgccgttgt aaaactccat gatgtcgatc
     3241 tcgccgttcg agggccactc gccgctcacg ccgagggtcc accacgcagg ccacatgccg
     3301 gccgacgtcg ggatgcgtcc gcgcatttcg aagcgcccgt attgccagct ctgaagcccg
     3361 ctcgtattca agctgctcga agtgtattcg gcgtattgac gcgacgtttt ccagtcgctg
     3421 ctaccgctgc tgtagttcgg gttctgaacg cgttctttgc gcccc
//
LOCUS       DASTKS010001021         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_607898, whole genome shotgun sequence.
ACCESSION   DASTKS010001021 DASTKS010000000
VERSION     DASTKS010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607898"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFQ35_21570"
     CDS             <1..243
                     /locus_tag="VFQ35_21570"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET9933315.1"
                     /translation="RASLEELNGLPPASVVTAEIDPLRDDGSAYAGRLQAAGVPVQAR
                     DYLGVTHEFFGMGVVLDKAKDAEAFVASSLQATFSK"
     gene            272..1651
                     /locus_tag="VFQ35_21575"
     CDS             272..1651
                     /locus_tag="VFQ35_21575"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HET9933316.1"
                     /translation="MLKTALIAFSIFGLTATLAGGCTSSDDDDSTSSGGRTASGGGSS
                     TRGGAANGGSSTRGGTSNASGGEGGNAGEGGNAGESGNAGEGGSAGERADLPVVLGGP
                     AAGKRDSKLEAVAVFPGPAQPVGIAISKKGRVFLSFPRWEDPVPYTVVEWKSGAMAPF
                     PDAKTTVFDPANPASTDPATHFVSAQAIVFDELDRLWILDPGSVNFSAVLNGAPKLWG
                     YDIDTGKLVAAISFPNDVAMKRSALNDVRFDLKRGPGGTAYITDSGAGGIVVVDLASG
                     LSWRHLDEHPSVLPTPGLTAMTEGRPFIQHKRSGEMAAPDFRSDGIALSPDGATLYYT
                     AVMSHEIYSVPTALLADPTTDEQVLGAAVKMVAQKPSGNDGIICDALGRIYTTDVEDN
                     SIRRVDTATGDVTVVARDERLIWPDTLAFGNGDLYVTVNQLPRQPSYRGGKDERQPPY
                     ALFRLALTD"
     gene            complement(1696..>2061)
                     /locus_tag="VFQ35_21580"
     CDS             complement(1696..>2061)
                     /locus_tag="VFQ35_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933317.1"
                     /translation="SAKERDREIVEATLTTFEATLASGIRDRSWERQSESALASVFTS
                     PALAGSRLGSINCVESLCKIVVQYDRQDHSDPMEVSERVLAPFNAAAFFHHSPSTNET
                     YFYVARDGQPLPNLAPPAG"
BASE COUNT          388 a          668 c          676 g          329 t
ORIGIN      
        1 cgcgcgtcgc tcgaggagct caacgggctg ccgccagcga gcgtcgtgac cgcggagatc
       61 gatccgctgc gcgacgacgg aagcgcctac gcgggccgcc tccaagctgc cggcgtgccg
      121 gtgcaagcga gagactacct cggagtgact cacgagtttt tcggcatggg cgtcgtgctc
      181 gacaaagcca aagatgccga ggcattcgtc gcgagttcgc tccaagcgac gttctccaag
      241 tagcccacct gcgcgacctc gaaggatcac catgctcaag actgcactca tcgcgttctc
      301 gattttcggg ctcacggcga cgctcgccgg tggctgcacc agctccgacg acgacgattc
      361 gacctcgtcg ggcggccgta ccgctagcgg cggcggcagc tcgactcgcg gaggagccgc
      421 caacggcggt agctcgactc gcggcggtac ctcgaacgcc agtggcggcg agggcgggaa
      481 cgcgggcgag ggcggcaacg ccggcgagag cggcaacgcc ggcgagggcg gtagtgccgg
      541 agagcgcgcc gacttacccg tcgtactcgg cggccccgcc gcgggaaaac gggatagcaa
      601 gctcgaggca gtcgcggttt tccccggccc cgctcaaccc gtaggcattg cgatatccaa
      661 gaaggggcga gtcttcctgt ccttcccgcg ctgggaggat ccggtcccgt acacggtcgt
      721 ggaatggaag agcggcgcga tggcgccgtt cccagacgcc aagacgaccg tattcgatcc
      781 tgcgaacccg gcgagcacgg atccggcgac gcacttcgtg agcgcgcagg ccatcgtctt
      841 cgacgagctc gacaggcttt ggatcctcga cccagggagc gtgaacttct cggcggtctt
      901 gaatggtgcg cccaagctct gggggtacga catcgatacg ggcaagctcg tcgctgcgat
      961 ttcgttcccc aacgacgtcg cgatgaagcg cagcgcgttg aacgacgtcc gcttcgactt
     1021 gaagcgcggc ccgggcggca cggcctacat caccgacagc ggcgcgggcg gaatcgtcgt
     1081 cgtggatctc gcgagcggcc tttcgtggcg acacctcgac gagcacccgt cggtactgcc
     1141 gacaccaggg ttgacggcga tgaccgaagg acgtccgttc atccaacaca aacgctcggg
     1201 agaaatggcg gcgcccgact tccgctccga cggcatcgcg ctgagcccag atggcgcgac
     1261 gctctactac accgccgtga tgagccacga gatctacagc gttcccaccg cgctactcgc
     1321 ggatccgacg accgacgaac aggtgctcgg cgccgccgtg aagatggtgg cccaaaagcc
     1381 gagcggcaac gacggcatca tctgcgacgc cttgggacgc atctacacga ccgacgtcga
     1441 agacaacagc attcgtcgcg tcgacacggc caccggcgac gtgaccgtcg ttgcgcgaga
     1501 cgagcgcctg atttggcctg acacgctcgc gttcggaaac ggcgacctct acgtgaccgt
     1561 gaatcagctc ccgcgtcagc cgagctatcg cggcggcaag gacgagcgac aacctccgta
     1621 cgcgctgttc cgactggcac tcacagactg agggtagcca agggtggccg ggcagatgcg
     1681 gctcggccac cttcgctaac cggcaggcgg agcgagattc ggcaatggct gaccgtcgcg
     1741 agcgacgtag aagtaggttt cgttcgtcga gggagagtga tgaaagaagg ctgccgcgtt
     1801 gaatggcgcc aataccctct cgctcacttc catcggatcc gagtgatcct gccggtcgta
     1861 ctgcacgacg atcttgcaga gtgattcgac acagttgatg gagccgagtc gcgagccagc
     1921 gagagcagga ctcgtaaata cggaggcgag cgcgctttcc gattggcgtt cccagcttcg
     1981 atctcggatt ccggaagcga gcgttgcctc gaacgtggtc aacgtcgcct cgacgatctc
     2041 acgatcgcgc tcctttgcgc t
//
LOCUS       DASTKS010001022         5801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1054772, whole genome shotgun sequence.
ACCESSION   DASTKS010001022 DASTKS010000000
VERSION     DASTKS010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054772"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VFQ35_21585"
     CDS             <1..1359
                     /locus_tag="VFQ35_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933318.1"
                     /translation="VIEQAKRRGIELEPGEVSFGLGWVQVKDAKLSLVGLPGIRAKAG
                     IIDVELARMTPQRFTLNDVKVEATGSASGLQGDLSSWLRAHEAQFTEPVFMKPVEVQY
                     RQQPSGGASMAITGGEISASHTELDVKASRFTIEGHELGAMKLTGKKSHVELAMTLGL
                     SDIKNPTLDIEATVADGWKLHVALAPVLLGRLGAALNTAMPLPAVTASGTFDLDVPRV
                     LTPGAHPSGRVDATLKGYVPPHPVELDGFVFGDTTELGSKFTVVPEQLKLLLEETRVK
                     AGSFVLQGAGSVAVEAGGPRLRLNLSGALPCGALAGAAAQTRLGTALGRLTGKAARET
                     LSGSVGVHVVVEADVLNLDNPRVLKTITPGCGLKPLTLAELRALGELLPDALDPRVAD
                     DLAKLLKNPLALPQLPPDTNLDLSRLPKLPLPVLPLPAPPTPAPAKPSKPSTARPPAS
                     AH"
     gene            1390..2436
                     /locus_tag="VFQ35_21590"
     CDS             1390..2436
                     /locus_tag="VFQ35_21590"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933319.1"
                     /translation="MASILILGAGMMGTAFAWPLVDRGHSVRLVGTHLDDEFVDSIRA
                     HRVHPKLGLEVPDGVTAYPNVELESALAGVDAIVLGVSSAGVVWAAERLAPLLERALP
                     LAMISKGLAFHDDSLHTLPDEFASHLPSKLRAEVHPVGVAGPCIAGELARRVPTSVVL
                     TARSLEAARAFAELCRGAYYHVWCSEDVVGVEVCAALKNAYAMGIALAAGMHAARGGK
                     EGSIALHNYEASVFAQSIWEMQKLIALLGGDPKQAAWLPGVGDLDVTCNGGRTGRFGQ
                     LLGQGLGRDESVARMHGATLECLEILAVLRRALDHFVARGRISTSDFPLARHLAEIAL
                     DDAPVAMPFDRFFR"
     gene            2660..3001
                     /locus_tag="VFQ35_21595"
     CDS             2660..3001
                     /locus_tag="VFQ35_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933320.1"
                     /translation="MQPIVSLAFIAVYCGILWRWSSDVRSRLGPRLESYFVARSRRRH
                     GSDSRPEGALGAAQVALLFLTTFLPVIALYAAAQVRAISWPRANTMLIFSLLIPAARF
                     SAAEREKRERS"
     gene            3135..4691
                     /locus_tag="VFQ35_21600"
     CDS             3135..4691
                     /locus_tag="VFQ35_21600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933321.1"
                     /translation="MRAAVPETQWHRRLRRARRFFLGEPSGYVIAAATTGAITLVSYL
                     AMPHADIAHVMILHLLAPVLISTRFGLAVSTFSAVSSVLAFDYFCVPPIFAFAFPDSH
                     SVITLVSMLLGALTLCFSLQRLRHQRTLAREREGQTRALCELSLDLSELRRSPEVAEI
                     AERHLGLLFGSDTRVLLRDANGQFELGRLPAHEHEAVELALARLETAFNDGAGKTILF
                     EPIGGGPVPLGVVRARFDEDGLSSGELKMLLAACADRIALSLERNALSQAALNAEVSA
                     ETERLRNELLSAVSHDLKTPLSSILAAGTALLNRAPTHDENIELLQTIVDEAERLNTL
                     LTNLLSVTRLESGRVRLRRTPEALDDLIFSVLSRLSSRLEGRPVEVDVPDDLPLVPVD
                     AVLIDQVLMNLIENVLRYTPAGSPVALSVRERGEEVVLEVCDRGPGIAADERDKVFER
                     FFRGKQAKRNDGGTGLGLTICRAVARAHGGRIAVRARESGGTTVEFALPCPSRHVALL
                     TAEPGERPIA"
     gene            4688..5374
                     /locus_tag="VFQ35_21605"
     CDS             4688..5374
                     /locus_tag="VFQ35_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933322.1"
                     /translation="MTRSVLVVEDELPMRRVLRSALGGQGYRVWEAGSAAEGLLAFAA
                     RAPEAILLDLGLPDADGFEVVRRVRETSEVPIVVISARGAERDQVRALDDGANDYVTK
                     PFREAELLARLRAALRSASRLGPAADALVIGPLCIRSLERRVFLNDREVALTPTEFSL
                     LQVMAREAGRVVTHRQLLRDVWGAANTDDVQYLRVFMKQLRRKLEQDPTKPQLLITAP
                     GVGYRLKGPE"
     gene            5556..>5801
                     /locus_tag="VFQ35_21610"
     CDS             5556..>5801
                     /locus_tag="VFQ35_21610"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933323.1"
                     /translation="MTDPSLPEVVKQVAELKTSAGDLTTAANLVWTLVCGFLVMFMQA
                     GFALVEAGLCRKKNAAHVMSMNFLVYAIGILGFWTIGF"
BASE COUNT          951 a         1910 c         1874 g         1066 t
ORIGIN      
        1 gtcatcgaac aagccaagcg acgcggcata gagctcgagc cgggcgaggt gtcgttcggg
       61 ctcggctggg tccaggtgaa ggacgcaaag ctctcgctcg tcgggttgcc gggcattcgg
      121 gccaaggccg ggatcatcga cgtggagctc gcccgcatga ccccgcagcg cttcacgttg
      181 aacgacgtga aggtcgaagc gaccggcagc gcctcggggc tgcaaggcga tctttcgagt
      241 tggctccgag cgcacgaagc gcagttcacc gagcccgtgt tcatgaagcc ggtggaggtc
      301 cagtatcggc agcaaccctc cgggggcgcc tccatggcga ttacgggagg tgagatcagc
      361 gcgtcgcaca ccgagctcga cgtaaaggcc agccgcttca cgatcgaagg acatgagctc
      421 ggcgcgatga agctcacggg gaagaagagc cacgtggagc tcgcgatgac actggggctc
      481 agcgacatca aaaacccgac cctcgacatc gaggcgaccg tggcagacgg atggaagctg
      541 cacgtcgcgc tcgcaccagt tctcctcggt cggctcggcg cagcgctgaa caccgcgatg
      601 cccctccccg cggttacggc cagcgggacg ttcgatctgg acgtaccgcg cgtgctcacg
      661 cccggcgcac acccgagcgg ccgcgtggac gccacattga aaggctacgt ccctccgcat
      721 ccggtggagc tcgacggctt cgtgttcggc gacacgacgg agctcggctc gaagttcacg
      781 gtcgtgccgg agcaactgaa gctgttactc gaagagactc gcgtgaaggc gggctccttc
      841 gtactccaag gcgcaggctc ggtcgcggtc gaagcgggag gtccgcgact gcgactgaac
      901 ctttccggcg cccttccctg cggcgccttg gccggcgctg cagcgcaaac gcggctcggc
      961 acggcgctcg gccgcctaac agggaaagcc gcccgcgaaa cgctgtccgg ctcggtgggc
     1021 gtacacgtcg tcgtcgaggc ggatgtcttg aacctcgata acccgcgcgt gctcaagacc
     1081 atcacgcccg gctgtgggct caagccgctc accttggcgg agctccgtgc gctcggcgag
     1141 ctcttgcccg acgcactcga cccgcgcgtt gccgacgatc tcgccaagct actgaaaaac
     1201 cccctggctt tgcctcagct ccctcccgac acgaatctcg atttgtcgcg cctccccaag
     1261 ctccctctcc ccgtcctccc gttacccgcc ccaccgactc cagcccccgc gaagccgagc
     1321 aagccgagta ctgcgcgccc gcccgctagc gcacattgac ggcatccaac aattgtcgaa
     1381 aaagaggcca tggcatcgat cctgatcttg ggcgccggca tgatgggcac cgcgttcgcc
     1441 tggccgctcg tcgaccgcgg tcacagcgtg cgcttggtgg gcacacacct cgatgacgag
     1501 ttcgtagatt ccatacgcgc gcaccgggtc caccccaaac tcggcctgga ggtgccggat
     1561 ggcgtcactg cctatcccaa cgtcgagctc gaaagcgcgc tcgccggagt cgacgccatc
     1621 gtgctcggag taagctctgc gggggtcgtt tgggcggcgg aaaggctcgc ccccttgctc
     1681 gagcgcgcgc tgccgctcgc catgatttct aaagggctcg cgtttcacga cgactcgctg
     1741 cacaccttgc ccgacgagtt cgcgagccat ctcccgtcga agcttcgggc agaggttcac
     1801 ccggtcggtg tcgcggggcc gtgcatcgcg ggagagctcg cgcggcgcgt gccgacctca
     1861 gtcgttctca ccgcgcgctc tctcgaagca gctcgggctt tcgctgagct ctgccgcggc
     1921 gcctactatc acgtctggtg ctccgaagac gtggtgggcg tcgaggtgtg tgcggcgctg
     1981 aagaacgcct acgccatggg gatcgcgctc gcagccggca tgcacgcggc gcgcggcggc
     2041 aaggaaggga gcatcgcgct tcacaactac gaggcctcgg tgttcgcgca atcgatctgg
     2101 gagatgcaga agctcatcgc gctcttgggc ggcgatccga agcaagcagc ttggctgccg
     2161 ggtgtgggcg acttggacgt gacgtgcaat ggggggcgca ccggtcgatt tggccagctc
     2221 ctcgggcagg ggctcggacg ggacgagtcc gtcgctcgca tgcacggcgc caccctcgag
     2281 tgcctcgaga ttttggcggt gcttcgccga gcgctggatc atttcgtcgc gcggggccgc
     2341 atttccacga gcgatttccc gctggcgcgc cacctcgccg agatcgcgct cgacgatgcg
     2401 cccgtcgcga tgccgttcga ccgcttcttt cgttgaagca gcaaggcgca tcgcgacctc
     2461 gatgtttcga gcacgtctcg gccgacgcaa ccgtggataa aggcgccctc acgctttcct
     2521 tacgggattc cacatggact cgatggggct agagcctcat cattctcgag aatctcggag
     2581 ccgcttctga cccgggctgt ctcgaggccc gggcgcggcc aattgaatca cgagtctctc
     2641 gccccaccgt gatgagcgga tgcaaccgat cgtctcgctc gcgttcatcg cagtttactg
     2701 cggaatactg tggcgctggt cttcggacgt gcgcagcagg ctgggccccc ggctcgagag
     2761 ctacttcgtc gcacgctccc gtcggcgcca tggctcggac tcgcgacccg aaggggcgct
     2821 cggtgcggcc caggtggcgc tgctcttcct cacgaccttc ctgcccgtga tcgccctcta
     2881 tgccgctgcg caagttcgcg caatcagctg gccgcgagcg aatacgatgc tcattttctc
     2941 gctgctcatc ccggcggcgc ggttcagcgc cgctgaacgc gaaaagcgag agcgttcctg
     3001 agcgcttcgc gcattcgcgc cgctgaccgc gagcattgtt tccgttaggt tgcgtgcgat
     3061 gcccagcatt tggcgccaga gtagcggtgc gccgcctcat gtttcgggct ctgtaatgcg
     3121 gcgcacctac gccgatgcgt gctgccgtcc ccgagactca atggcaccgc cgccttcgcc
     3181 gggcgcgtcg cttctttttg ggggagccgt cgggttacgt cattgccgcc gcgaccaccg
     3241 gggccatcac gctcgtttcg tacctggcga tgccgcacgc ggatattgcg cacgtcatga
     3301 ttctgcacct gctcgcgccg gtgttgatct cgacgcgctt cggtctcgcc gtgtcgacgt
     3361 tcagcgcggt ctcgagcgtg ctcgccttcg actacttctg cgtcccgccc atctttgcgt
     3421 tcgcgtttcc tgacagccac agcgtcatca ccctggtttc gatgctgctc ggagcgctca
     3481 cgctgtgctt ttcgcttcaa cgcctgcgcc accagcgcac tctcgcgcgc gaacgagagg
     3541 gtcagactcg agcgctctgc gagctcagcc tggacctttc ggagttgagg cgctcgcccg
     3601 aagtggccga aatcgccgag cgtcatttag gcttgctctt cggtagcgac actcgggtcc
     3661 ttttgcgtga cgcgaacggg caattcgagc tcggccggct gccggctcat gaacacgaag
     3721 cggtggagct cgcgctcgcg cgcctcgaga ccgccttcaa cgacggcgcc gggaagacca
     3781 ttctcttcga gccgattggc ggcggcccgg ttccactcgg ggtcgtgcgc gcgcgcttcg
     3841 atgaagacgg cttgagctca ggcgagctga aaatgctgct cgcggcttgc gccgatcgaa
     3901 tcgccttgtc gctcgaacgc aacgccttgt cgcaggcggc gctgaacgcc gaggtcagcg
     3961 ccgaaaccga gcggttacgc aacgaactcc tgagcgccgt ctcgcacgat ctcaagactc
     4021 cgctctcttc gattctggcc gccggcaccg cgctcctgaa ccgcgcgccc acccacgatg
     4081 aaaatatcga gcttttgcag acgatcgtcg acgaggcgga gcgactcaac accttgctca
     4141 cgaacctgct cagcgtcacc cggctcgaga gcggtagggt gcgattgcgg cgcactcccg
     4201 aagcgctcga cgacctgatt ttcagcgtgc tgtcacggct ttcgagccgg ctcgaagggc
     4261 gtccggtcga ggtcgacgtt cccgatgatc tgccgctcgt gccggtcgac gcggttctca
     4321 tcgatcaggt gttgatgaac ctcatcgaga acgtgttgcg ctacacgcca gccggctcgc
     4381 ccgtcgcact cagcgtgcgg gaacgcggag aagaagtcgt cttggaggta tgcgaccgcg
     4441 gtcccgggat cgcagccgac gaacgcgaca aggtcttcga gcgatttttc cgaggcaagc
     4501 aggcgaagcg caacgacgga gggaccgggc tcggactcac catctgccgt gcggtcgccc
     4561 gagcgcacgg cgggcgcatc gccgtgcgag cacgtgagtc cggtggaacg acagtcgaat
     4621 tcgcgctgcc gtgtcccagc cggcacgtcg cgctgctcac ggccgaacct ggagagaggc
     4681 ccatcgcatg acacgctcgg ttctggtggt cgaggacgaa ctgccgatgc ggcgagtttt
     4741 gaggagcgcc ttgggcgggc agggctaccg cgtctgggag gctgggagcg ccgccgaggg
     4801 actgctcgcc ttcgccgcgc gcgcgccgga agccattctg ctggatttgg gtttgcccga
     4861 cgcggacggc ttcgaggtgg ttcgtcgcgt ccgcgagacg tccgaggtac caatcgtcgt
     4921 gatttctgcg cggggagccg agcgcgacca ggtgcgggcg ctggacgatg gcgcgaacga
     4981 ctacgtgacg aaaccgtttc gagaggcgga gcttctggcg aggctcagag cagcgctgcg
     5041 cagcgcgagc cgacttggac ctgctgcgga cgctctagtc atcggtcccc tctgcattcg
     5101 ctcgctcgag cggcgcgtgt tcctgaacga tcgcgaggtc gcgctgactc cgaccgagtt
     5161 cagcctcttg caagtgatgg cgcgcgaagc ggggcgcgtc gtgacccatc gccagctgct
     5221 gcgcgacgtg tggggcgcgg ccaacaccga cgacgtccag tacttgcgcg ttttcatgaa
     5281 acaattgcgg cggaagctcg agcaagaccc gaccaagccg cagctcctaa tcacggcacc
     5341 gggcgtggga taccggttga agggtcctga gtaagccctc tttcctcgga aatttcatgg
     5401 cagccggcgc aactatcttg aagttccggc atctaaaccg agggttcgta ctccgcataa
     5461 acgaatcttt acgcggtccg tgcgagggag aagggcccga gaaatgcggc tgaaacgcgc
     5521 gcgccgctac tcgcgagtga aggagttttc tggccatgac cgacccctct cttcctgaag
     5581 tcgtgaagca ggttgccgag ctgaagacca gcgccggcga tctgaccacc gctgcgaatt
     5641 tagtctggac gctggtttgc ggcttcctcg tgatgttcat gcaagccggg tttgcgctcg
     5701 tcgaagccgg cttgtgccgg aagaagaacg ccgcccacgt gatgtcgatg aacttcctgg
     5761 tctacgcgat cggaattctc gggttttgga ccatcgggtt c
//
LOCUS       DASTKS010001023         6853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1279205, whole genome shotgun sequence.
ACCESSION   DASTKS010001023 DASTKS010000000
VERSION     DASTKS010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279205"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /gene="purB"
                     /locus_tag="VFQ35_21615"
     CDS             <1..1278
                     /gene="purB"
                     /locus_tag="VFQ35_21615"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9933324.1"
                     /translation="KDFVELWNEKTRYNTWLEVELAACDAMESAGLVPHGTARAIRAK
                     NVALDPRRIEEIEATTRHDVIAFLTHVEELAGEPARWLHRGMTSSDVLDSSLALLLVR
                     AADMLIARCEKLVAALASRAREHARTPMIGRSHGIHAEPVTFGVALAGHLAEVKRGHV
                     RLVRAREEIAVGKIAGAVGSYAHLSPAIEEQALGALGLRPETVSTQVVARDRHAAFFS
                     ALAVVATAIERLATNVRHWQRTEVGEAEEKFSKGQKGSSAMPHKRNPVLSENLCGLAR
                     IVRSAVVPALENVALWHERDISHSSVERMIAPDATTTLGFMLERATGLVEGLVVYPAN
                     LKRNLDLTGELFFSEAVMLALVHKGLPRQRAYEMVQRSAMRAFQGEGSFREFLKADAE
                     IQKLLQAAEIDECFDLDHALRYAETLVERAIAS"
     gene            complement(1339..2058)
                     /locus_tag="VFQ35_21620"
     CDS             complement(1339..2058)
                     /locus_tag="VFQ35_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9933325.1"
                     /translation="MWRQSRIAVVVPAYCEARIIGRMLRRLPGYVDAVFVVDDASPDE
                     TSSAALAVGDPRVVALRHAVNRGVGAAIVTGYRAALEAGSDVAAVMAGDDQMHPDDLE
                     AVLAPVVGGLADYVKGNRFVHPEARRMPVLRRLGGEMLSFSTRRATGLSVSDTQCGFT
                     AISAPMLRRLPLGELWPRYGYPNDLLGLLSSHGARVREVPVRPVYADETSGLRAFHVA
                     SILGVIARRYLREKRRASLEP"
     gene            complement(2062..2547)
                     /locus_tag="VFQ35_21625"
     CDS             complement(2062..2547)
                     /locus_tag="VFQ35_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933326.1"
                     /translation="MSQLSALVRPFLVVCALSAAACGSDGDANPNVDDPLANEDVVRC
                     DMPADHACREYNRGQQGDATAFVDLPAARKSCVGGWPGGPPDAGVFGAGSCSKDDALG
                     RCVTQSQTIAVLVTIDYFYTGFADGTATTDQLQQVCANVQANAAAHGVQLTSVFQSPP
                     F"
     gene            complement(2637..3218)
                     /locus_tag="VFQ35_21630"
     CDS             complement(2637..3218)
                     /locus_tag="VFQ35_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933327.1"
                     /translation="MKATTAPARRTLLLLAALSTIVGCGNNVGATKPVKDCDEACEVR
                     VAARGLRETLKLAYNLTLNGNPVGAQDESHACLAGNVRVRGEAYSNAEQGSTEVDLDY
                     DFSDCAYHQVDANEEENYALTFNGTVAESGVLTVQPTAPTALIITSDGIAIDGTVFDP
                     PLEFHEASCEVRLQQSGNKLSGTLCGRDVGVSL"
     gene            complement(3221..5743)
                     /locus_tag="VFQ35_21635"
     CDS             complement(3221..5743)
                     /locus_tag="VFQ35_21635"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9933328.1"
                     /translation="MSEFDTVLSSLHLRTSPVRGASVWSKRALLVRSCVFTNVVFSLV
                     AFAPVARAQNDPQRPPAQDQSPPISPAIEATVVPPKLLSEAEVPYPSSAHGEPIVRLR
                     VTVNADGSVREARAIDAPEPFAVTAEDAVRKWQFQPATRNGRPIVATIAVEITFHEPV
                     LEPEPEIAPEPPVDGHDVKAPAPRKPMEVLVLGDKPEPSRSASLTRAEVRQIPGTFGD
                     PFRAIEALPGITPIVSGLPFFFVRGAPPGNVGYFLDGVSLPLLFHVGAGPSVIHPGLI
                     DRVDLYPGGYPARYGRFAGGIVSGETAAPRGSLHGEYNLRLFDAGALLEVPFAEGRGS
                     ALVAGRYSYTALLLSLLSPDVSLSYWDYQARVGYDVSKRDRVTVFAFGSYDFLGQKTA
                     DSTLTLFGTEFHRVDVRHDHEFVSGARLRNALTVGIDRSETQEGRFVRARSAGARTEL
                     ELPLAPNARLRAGTNLSLSYYDVELGPADLSTTANNIASLFPARTDVAVGARADVVWR
                     LLPRLEATPGVRVDLYGSEGATALGIDPRLALRSELSERVRLKAALGVAHQPPAFVVP
                     LPGFQPGGLRGGLQRALQESFGIEADLGQGTTASATVFQNAFFAMSDPLGASEPRAGG
                     CPPGTFPTDTLAGDRLTDPTTPPPCRDRFDRGTLGPDNSGGGGQASTGGGNGSRILRA
                     FEVRTLGHAYGLELYLKKSLTSKLGGFVSYTLSRSTRSFENRSYVANFDRTHVLNTAL
                     AYDLGRGFRAGGRVTFYTGLPAVATPDNPDGGRLPSFFRLDLRLEKRWQLGREAWISA
                     VAEWLNATLSKEAVSTRCTLAGCEARTIGPITIPSLGVEGGF"
     gene            complement(6064..6264)
                     /locus_tag="VFQ35_21640"
     CDS             complement(6064..6264)
                     /locus_tag="VFQ35_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933329.1"
                     /translation="MLERLRAPELGSSLETATLRKLDSDALGVADVLASAPRRAELTA
                     GSDDSLGSASALRSGSDMAVGS"
     gene            6305..>6853
                     /locus_tag="VFQ35_21645"
     CDS             6305..>6853
                     /locus_tag="VFQ35_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933330.1"
                     /translation="MANPPRPMVVVYSGDRERPDRWWDEEAGPSRSLLLDADTPVPPD
                     PALSTRELRPPPVARFAEPESPGAEGDPASVKQFSTMIPPAGSATDTSETEAPVALSS
                     AARALPLPLHGAMRISEPPRPSEADLLRIKRERASTVRRAAVIIVGAFLSAYFVTQRV
                     ASRRNEAARASAEKAAQLVARKA"
BASE COUNT         1196 a         2230 c         2306 g         1121 t
ORIGIN      
        1 aaagatttcg tcgagctctg gaacgagaaa acccgataca acacctggct cgaagtggag
       61 ctcgccgcgt gtgacgcgat ggagagcgcc ggcctcgtgc cgcatgggac cgcacgagcc
      121 attcgggcga agaacgtagc gctcgacccg cgacgcatcg aggaaatcga ggcgacgact
      181 cgtcatgacg tcattgcctt tttgacccac gtcgaggagc tcgcgggaga gccggcgcgc
      241 tggttgcatc gcggcatgac ctcgagcgac gtcctcgact cgagcctcgc gctgctcttg
      301 gtgcgagccg ccgacatgct gattgctcgc tgcgagaagc tcgtcgccgc gctcgcctcg
      361 cgcgctcgag agcacgcccg gactccgatg attgggcgct cgcacgggat tcacgccgag
      421 ccggtgacct ttggcgtcgc gctcgccggc caccttgcgg aggtgaagcg cggccacgtg
      481 aggctcgtcc gcgcgcgcga agagatcgca gtcggaaaga tcgccggcgc ggtgggaagc
      541 tacgcacatc tgtctcccgc catcgaagag caggccctcg gcgcattggg gttgcgccca
      601 gagaccgttt cgacgcaggt ggtcgcgcgc gaccgtcacg ccgccttttt cagcgcgctc
      661 gccgtcgtcg cgacggcgat cgagcggctt gcgacgaacg tgcggcattg gcaacgcacc
      721 gaggtggggg aagccgagga gaagttcagc aagggccaga agggctcgag cgcgatgcct
      781 cacaagcgga atcccgtgct cagcgagaac ctgtgcggcc tcgcgcgcat cgtgcgctcc
      841 gcagtcgtgc cggcgctcga gaacgtcgcg ctgtggcacg agcgagacat ctcgcattct
      901 tccgtcgagc gcatgattgc gccggacgca accactacgc tcggattcat gttggagcgc
      961 gcaaccggcc tcgtcgaagg gctcgtggtt tatcccgcca acttgaagcg gaacctcgac
     1021 ctgaccggcg agctcttctt cagcgaagcg gtgatgctcg ccctcgttca caagggcctg
     1081 ccgcgccagc gcgcctacga aatggtgcag cgcagcgcga tgcgtgcgtt tcaaggcgag
     1141 ggatcgttcc gtgagttttt gaaggccgac gcggagattc aaaagctgct gcaggcagcc
     1201 gaaatcgacg agtgcttcga cctcgatcat gctctccgtt acgcggagac gctggtcgag
     1261 cgagcgatcg cttcttgaat ctggccgtcg ccgcgcgtgg gtgccgctga gcgcgcccac
     1321 gcgcaagctc ggtgcgcgct acggctcgag gctcgcgcgg cgcttctcgc gaaggtagcg
     1381 ccgcgcgatg actcccagga tgctcgcgac gtgaaacgcg cgcaggccgc tggtttcgtc
     1441 cgcgtagacg gggcgaacgg gcacttcgcg cacgcgcgcg ccgtggctgc tcaggagccc
     1501 gagtagatca ttcgggtatc cgtagcgggg ccacagctcg ccgagcggga gccgccgcag
     1561 catcggcgca gagatggccg tgaagccgca ctgcgtatcg ctcaccgaga gccctgtcgc
     1621 ccgtcgtgtc gagaacgaca gcatctcgcc gccgagccgc cgcaacacgg gcatgcgacg
     1681 tgcctccgga tggacgaacc ggttcccttt gacgtagtcg gcaagcccgc caacgacggg
     1741 cgcgagtacg gcctcgaggt cgtcggggtg catttgatcg tcgccggcca ttaccgccgc
     1801 gacgtccgag cccgcttcga gcgcagcgcg gtatcctgtc acgatggcag cgccgactcc
     1861 tcgattcacc gcatggcgca gcgcaacgac gcgcgggtca ccgaccgcga gcgcggcgct
     1921 ggaggtctcg tcggggctcg cgtcgtcgac cacgaacacg gcgtcgacgt agccgggaag
     1981 gcggcgcagc atgcgtccga tgatgcgcgc ttcgcagtac gcgggcacga cgacggcaat
     2041 gcgactctgc ctccacatgg gtcagaacgg cggagactga aatacgctcg tgagctggac
     2101 gccgtgcgcc gccgcattag cctgcacgtt ggcgcagacc tgctgaagct ggtcggtggt
     2161 cgcggtgccg tcagcgaacc ccgtgtaaaa gtagtcgatc gtcacgagca cggcgatggt
     2221 ttgcgactga gtaacgcacc gtccgagtgc gtcgtctttg ctgcaactgc cggcaccgaa
     2281 tactcccgca tcgggaggcc caccgggcca tccccccacg cagctcttgc gggccgccgg
     2341 caagtcgacg aacgccgtcg cgtccccctg ttgcccgcgg ttgtactcgc ggcaagcgtg
     2401 atctgccggc atgtcgcagc gaacgacgtc ttcgttcgcg aggggatcgt cgacgttggg
     2461 attcgcatcg ccgtcgcttc cgcaggcggc ggcgctcagc gcacagacga cgaggaaagg
     2521 tcgaaccaga gccgaaagtt gtgacatggg cacgccgggc aacgctctag cagacctcgt
     2581 tccgtttaca acgccgctcc gcgtcggtgg gcgcgccctt cgagcctcgc gcgcctctat
     2641 agcgagacgc cgacgtcacg accgcacaac gtaccgctca gcttgttgcc gctctgctgc
     2701 aggcgcacct cgcacgacgc ttcgtggaac tcgagcggcg ggtcgaagac ggtgccgtcg
     2761 atggcaatgc cgtcgctcgt aatgatgaga gcagtcgggg cggtgggctg gaccgtgagc
     2821 acgccgctct cggcgacggt gccattgaag gtgagggcgt agttctcctc ttcatttgca
     2881 tcgacttggt gatacgcgca atccgagaag tcataatcaa gatcgacctc ggtcgagcct
     2941 tgctcggcgt tcgagtaggc ctcgccgcgc acccgcacgt tgccggcgag gcaagcatgc
     3001 gactcgtctt gggcgcccac ggggttgccg ttcagcgtga ggttgtaagc aagcttcaac
     3061 gtttcgcgca ggccgcgcgc cgccacgcgc acctcacagg cttcgtcgca gtccttgacc
     3121 ggtttcgtcg caccgacgtt gttcccgcaa ccgacgatgg tgctcagcgc cgcgagcagg
     3181 agaagcgttc gtcgcgcggg cgcggtcgtt gctttcatga tcaaaagcct ccttctactc
     3241 ccaggctcgg gatggtgatt ggcccaatcg tcctcgcttc gcagccggcg agggtgcagc
     3301 gcgtcgacac cgcctctttg ctgagggtcg cgttcagcca ctctgcgacc gcagaaatcc
     3361 aggcttcacg cccgagttgc cagcgctttt cgaggcgcaa atccaaccga aaaaacgagg
     3421 gcagcctgcc gccgtcgggg ttgtcgggcg tcgcgaccgc tggcaagcca gtgtagaagg
     3481 taactcgacc gcccgcgcga aagccgcgcc cgaggtcgta ggcgagcgcg gtgttcaaga
     3541 cgtgcgtgcg atcgaagttc gcgacgtagc tccggttctc gaagctccga gtggagcgcg
     3601 agagcgtgta cgacacgaat cctccgagct tgctggtgag cgattttttc aagtagagct
     3661 cgagcccgta ggcgtggccg agcgtgcgca cctcgaacgc gcgaaggatg cgcgacccgt
     3721 tgccgccgcc ggtcgacgct tggccgccac cgcccgagtt gtccggcccg agcgtgccgc
     3781 ggtcgaaacg atctcgacac ggcggagggg tcgtggggtc ggtgaggcga tcgccggcca
     3841 aggtgtccgt tggaaaggtc ccgggcggac aacctcctgc gcgcggctcg ctcgccccga
     3901 gcggatcgct catggcaaag aaggcgtttt gaaatacggt tgcgctcgcg gttgtccctt
     3961 ggccgaggtc ggcttcgatg ccaaagcttt cttggagcgc ccgttggagg ccgccgcgca
     4021 atcctccggg ctgaaaccct ggtaacggga cgacgaaggc cggaggctgg tgcgccacgc
     4081 cgagcgcagc tttgagtcgc acgcgttcgg agagctcgct tcgaagtgcg aggcgcggat
     4141 cgatcccaag cgcggtcgcg ccctcggagc cgtacaagtc cacgcgaacg ccgggcgtcg
     4201 cctcgagtcg gggcagtaga cgccagacca cgtccgcgcg cgcccccacc gcaacgtcgg
     4261 tgcgcgccgg aaaaagcgac gcgatgttgt tcgccgtcgt cgacaagtct gccggaccga
     4321 gctcgacgtc gtagtacgaa agcgagaggt tcgtgccggc gcggaggcgc gcgttgggcg
     4381 cgagcggcag ttcgagctcg gtgcgagccc ccgcgcttcg agctcgaacg aagcgcccct
     4441 cctgcgtctc cgagcgatcg atgccgaccg tgagcgcgtt gcgcaagcgc gcgccgctca
     4501 cgaactcgtg atcgtgtcga acgtcgactc ggtggaactc cgtcccgaac agcgtgagcg
     4561 tggagtcggc cgtcttttgt ccgaggaagt cgtaagagcc gaatgcgaag acggtcactc
     4621 gatcacgctt cgagacgtcg tagcccactc gtgcttggta gtcccagtaa ctcaacgata
     4681 catcgggaga gagaagcgaa agcaggagcg cggtgtacga gtaccgcccg gcgacgaggg
     4741 ccgaaccgcg cccttccgcg aacggaacct cgagcagcgc ccccgcatcg aacaatcgca
     4801 ggttgtactc gccgtgaagc gagccacgag gcgcagccgt ttcgcccgaa acgatgccgc
     4861 ccgcgaagcg cccgtagcgt gccggatagc cgccagggta caagtccacg cggtcgatga
     4921 gccccggatg aatcaccgac ggacccgccc cgacatgaaa tagaagcggg agtgatacgc
     4981 cgtcgagaaa gtaacccacg tttccgggcg gcgctccgcg cacgaagaag aagggcaacc
     5041 cggagacgat cggcgtgatt cctggtagcg cttcgatggc gcggaacgga tcgccaaacg
     5101 tgccggggat ttgccgcact tctgcgcgcg tgagcgaggc gctgcgcgat ggctcgggtt
     5161 tgtctccgag cacgagcacc tccatgggtt ttctcggcgc gggcgctttc acgtcgtgcc
     5221 catccacggg cggctccggg gcgatctcgg gttccggttc gagcacgggt tcgtgaaacg
     5281 ttatctcgac cgcgatcgtc gcgacgatgg ggcggccatt gcgcgtcgct ggttgaaatt
     5341 gccatttgcg cacggcatct tctgcggtca ccgcgaacgg ctccggagcg tcgatcgccc
     5401 gtgcttcgcg gacgctgcca tccgcgttca cggtcacacg caaccgcacg atcggctcgc
     5461 cgtgggccga actggggtag ggaacctcgg cctccgaaag tagcttcggc gggactaccg
     5521 tggcttcgat cgcgggcgaa atgggcggcg attggtcttg agccggcggt cgttgcggat
     5581 cgttttgagc ccgtgcaact ggcgcgaaag cgacgagcga gaagacgacg tttgtaaaga
     5641 cgcagcttcg aaccaaaaga gcgcgctttg accacaccga cgcaccgcga acgggcgaag
     5701 ttcgcaagtg caaagaagac agcaccgtgt caaactctga catcgatgat gtcggatacc
     5761 gaagagcgcc gacggcagcg ctgcgtcaat tgcagcagcc tctcacccga agcgtccacg
     5821 agctatacac tgattagtgt caagcattcg tggcgcatgg tgctcagcgt gggtcctgac
     5881 ggcaagaagc ttcctcagtg gtactgcccc cgctgctggg agcaacgtcg ccggcagacc
     5941 gctccctgac gccgttttcg ggacaggttt tctcgccagc ctgccaaaat ccgccctgcg
     6001 gtggccttgg tggaccgcgc gcgaccgaaa tacttgatgt gacggggaaa tccgagccac
     6061 gcttcagctg ccgacggcca tgtccgaccc cgatcgcagc gctgaagcgg agccgagcga
     6121 atcatccgaa cctgcggtga gctctgcccg ccgtggcgcg gacgccaaca cgtctgccac
     6181 accgagcgcg tccgaatcta gcttgcgcaa ggttgcggtt tcgagcgacg agccgagctc
     6241 gggcgcgcgc aagcgctcca gcattcgccc agcgcctgct gattggcgac cgagcgagcg
     6301 cgcggtggca aatcctccac gcccgatggt ggtcgtgtat tcgggtgatc gcgagcgccc
     6361 cgatcgttgg tgggacgagg aagcggggcc aagccgctcg ctgttgctcg acgcagacac
     6421 accggtgccg cccgatccgg cgctatcgac gagggagctg cgcccgccgc cggtcgcgcg
     6481 cttcgcggag cccgagtccc ccggcgcgga aggggacccg gcctcggtca aacagttctc
     6541 gaccatgatt ccgcccgcgg gaagcgcgac ggacacgagc gaaaccgaag cgccggtcgc
     6601 gctgtcgtca gccgcgcgcg cgttgccgct tccgctgcac ggcgcgatgc gcatctcgga
     6661 gccgccgcgg cccagcgaag cggatctgct tcgcatcaag cgcgagcggg cgagcaccgt
     6721 gcgacgcgcc gcggtgatca tcgtgggcgc gtttttgagc gcgtacttcg tcacacagcg
     6781 agtcgcatct cgccggaacg aagccgctcg cgcgagcgcc gaaaaagcag cgcagctcgt
     6841 cgcgcgcaag gcg
//
LOCUS       DASTKS010001024         7403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1725217, whole genome shotgun sequence.
ACCESSION   DASTKS010001024 DASTKS010000000
VERSION     DASTKS010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725217"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFQ35_21650"
     CDS             complement(<1..576)
                     /locus_tag="VFQ35_21650"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HET9933331.1"
                     /translation="MTGTISFGGVGSGMDTEGIVSGLIQADSGPLNALKTKQADTDSA
                     VSVLSNISSLLSTFRSSVNALSDARNVGSYTATSSSTAVAASANGAALPGAYDVEVLA
                     LAKEQRNYSQTFSAHDGALGLSGTLTLQVGSGTAVNVDVKATDTLDDIVNSINGKGGR
                     FTASVFNDGQNYRLQLRGLDTGAANAITIGGL"
     gene            complement(710..1531)
                     /locus_tag="VFQ35_21655"
     CDS             complement(710..1531)
                     /locus_tag="VFQ35_21655"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET9933332.1"
                     /translation="MALTINTNSASLTAQKNLASSQSALAGSFQKLSSGLRVTSAADD
                     AAGLAVSESMKSQIRSYTVAQRNAADGISMAQTAEGALGQVHDVLGRMRELAMQSSNG
                     SLGSTDRGYLDTEFSSLKNEIDRIQKSSKFNGVSLVNSTGATINFQVGLNNVTGSDQI
                     SVTFGGVDVSAISSATVANATAALAALGSIDTTIQNVSNSRAKFGTAMNRLNYATSSI
                     QTMSVNLSAANSRIVDVDVASETAAMSRNQVLSQAGVSVLAQANQLPQLALSLLR"
     gene            complement(1723..2550)
                     /locus_tag="VFQ35_21660"
     CDS             complement(1723..2550)
                     /locus_tag="VFQ35_21660"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET9933333.1"
                     /translation="MISVQTNVSSLETQKALGKTQAKLNQNFTRLSSGFRINTAADDA
                     AGLAISENMKMQIRSYTVAERNSNDALSMTQTAESVLGEMSDILGRMRELAMQGANGD
                     MTDTDRGYLDTEFTALKQEIQRMQQSAKFNGKQLLSSTASSITFQIGLNNTQSDQITL
                     TFGGLGLTNLLDATNNLAGATATNSLAALGRIDAALTTVSTNRAKYGATMNRLEVAVS
                     SIQTMRLNLSAANSRIRDVDVAEETAALSRNQILSQAGASVLAQANQLPQLALSLLK"
     gene            complement(2632..4542)
                     /locus_tag="VFQ35_21665"
     CDS             complement(2632..4542)
                     /locus_tag="VFQ35_21665"
                     /inference="COORDINATES: protein motif:HMM:NF014075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HET9933334.1"
                     /translation="MGQVSMSQAAARQLDGGRGRQSEDTDGASGAFPTAADMSQQFFE
                     ENLRHMALPALTLEAVRGAHSAGGLAGLELGVPVVNRQGTLLGQTPPEAELEYAGSLE
                     HTLIVVFGLGLGETARALRELTNEPIIVFEPDPGILRQVLELGPTALSDFDIVCTTHD
                     LTQIWPTIFGQRRQALVVSTPGYEDAFPGASADLRAAVAELVQRCRVNNETHRLRARD
                     WIEDVLANVELLSEHPGFLALGGKYRGVPAFIVGAGPSLGKNGHHLVQAQKKGIVFAL
                     NSSARALDRLGVTPQVVACMESIDVSHLLAEVSYLNRSIRAFSLSAHPKNLRVGAGPL
                     LALWEGISQLSLPLFQLTGYAGLAVSGSVSTLVFALAQRLGCSPIVFVGQDLAYTGGR
                     AYAPGTPYENSRVERSADGRNIKLDWCDTLRATHRLEGGFLQESEPLNETVAWGGDGH
                     VVSSVAFSAVRGWLESAAIVLGRESPETRLVNATEGGSRILGFDEVTLEALLSTLPDL
                     DITPESIVANAHAETEALPRARIAAWAENQADLAASVRHTARRIRRLSQTALTAAKRG
                     DPSVTTRLSRLDAAELELRKRVGSAPLLDAWSWAEVHGVMAGQTEVNQDNQDNAYTAL
                     ASEARLGAQLDA"
     gene            complement(4524..4775)
                     /locus_tag="VFQ35_21670"
     CDS             complement(4524..4775)
                     /locus_tag="VFQ35_21670"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HET9933335.1"
                     /translation="MLIIARRKGQRVVIGQDIEIVVTEVTRGSVKLGIVAPARTTILR
                     GELHDAVQAANIEAVNTISDPAELPLPLPKGVGPWGKSP"
     gene            complement(4898..5773)
                     /gene="flgL"
                     /locus_tag="VFQ35_21675"
     CDS             complement(4898..5773)
                     /gene="flgL"
                     /locus_tag="VFQ35_21675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HET9933336.1"
                     /translation="MRVTDGMKYLGVTRNLARLQTQNAQAQQQASTGLRLEKPSDDPV
                     AAAELARLRASLSNSAGHREAIQRAQGDAELAESTLSQVNELMASAKEAAMQGSNDSL
                     GVDDRKALATQVRGLRTELLKLANTRGTKGYIFAGSKTDAAAFDSNGAFRGDDQEQVI
                     DTGGSSPVAVSASGAKAFTAAGGRDVFADLDALATALDNNDRSGASAALDAIDAGATQ
                     VTRERTRIGLTASKLQASDGVLEQLGVDLGKQQANVGAVDPFEAYSKVTTLAQSLQQA
                     VQVSRQVLDLGDLWR"
     gene            complement(5796..7181)
                     /gene="flgK"
                     /locus_tag="VFQ35_21680"
     CDS             complement(5796..7181)
                     /gene="flgK"
                     /locus_tag="VFQ35_21680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HET9933337.1"
                     /translation="MSSSLSQLLYTARDGLTAQSYGLGVTGQNISNVNTPGYVRREAL
                     LETRALGDAGSVNAAGIRRVTDVFLERRQYETTGLSSAASEHDKDLANIEALFNDQSG
                     TGLGSSLDQVFKSFSALASNPSDPTTRDGVLDAADEFAKRVNDTANAIADAQKGLFQA
                     AQATVQEINQKAQEIAKLNEQIAQAEAAGQSAADLRDKRSQLLMSLSGMVDVRTVQSG
                     DSLTVQVSGATLVDGAAPRSLSIGLDATGAMQVLSSSGNGSTAQDVTKFLTGGKLAGI
                     REARDVDLVAVGKQLDQFAYDVGTAINTQHAAGFGLDGVTGRHLFDLTATATGAARAI
                     RLSDDVAGNPAAVAASSSAATLPGGSDNAALLSAMSRTPIASGGTRTASDAYGDIVGD
                     IASRRSASSQMLEAREAISAQVDSMHESMSGVSLDEEFVNLTKFQRAYQASARLLTTA
                     DQLLQDLIQRV"
BASE COUNT         1128 a         2299 c         2572 g         1404 t
ORIGIN      
        1 gaggccgccg atcgtgatgg cgttggcggc tccggtgtcc aggccacgga gctgcagtcg
       61 gtagttctgg ccgtcgttga agacggacgc ggtgaaacgg ccgcccttgc cgttgatgct
      121 gttgacgatg tcgtcgagcg tgtcggtcgc cttgacgtcg acgttcacgg cggtgccgct
      181 gccgacctga agcgtgagcg tgcccgaaag gccgagcgcc ccgtcgtggg cagaaaatgt
      241 ctgtgagtag ttgcgctgct ctttcgcgag cgcgagcacc tcgacgtcgt acgcgccagg
      301 cagcgctgcg ccgttcgcgc ttgcggccac tgcggtgctc gacgacgttg cggtatagct
      361 gccgacattg cgcgcgtcgg agagcgcgtt caccgagctg cgaaacgtgg agagcagaga
      421 gctgatgttc gagaggaccg aaaccgccga gtccgtgtcg gcctgcttcg tcttgagcgc
      481 gttgagggga ccgctgtcgg cctgaatcag gccgctcacg ataccctcgg tatccattcc
      541 ggatccgacg ccgccgaacg agattgttcc agtcattgtt ttggtctctc gagctctcct
      601 agaagagcgg catggatgac gaagccttga gctcctgctt ggcccacgcg ccgccagcct
      661 gcactcggct gacggcgcgt gagccgtccc aaacgctcaa tacgggagat tacctcagaa
      721 ggctgagtgc gagctggggc agctggttcg cttgcgcgag gaccgacacg cccgcttgcg
      781 agagcacctg gttgcgcgac atggcggccg tctcgctcgc gacgtcgacg tcgacgatac
      841 gcgagttcgc ggcggacagg ttcacgctca tggtctggat gctcgacgtg gcgtagttca
      901 gtcggttcat cgccgtaccg aacttggcgc gcgagttcga cacgttctgg atcgtcgtgt
      961 cgatgctgcc gagagccgct agtgccgcgg tcgcgttcgc caccgtggcg ctcgagattg
     1021 ctgacacgtc cacgccaccg aaggtgacgc tgatttggtc ggagccggtg acgttgttca
     1081 ggccgacctg gaagttgatc gtcgcaccag tggagttcac cagcgagacg ccgttgaact
     1141 tcgaggactt ctggatacgg tcgatttcgt tcttgagcga cgagaactcg gtgtcgaggt
     1201 agccacggtc ggtagaaccg aggctgccgt tggaggactg catcgcgagc tcgcgcatac
     1261 ggccgagcac gtcgtggact tggccgagcg cgccttccgc cgtctgcgcc atcgaaatgc
     1321 cgtccgcggc gttgcgttgg gcgacggtgt aggagcgaat ctgcgacttc atgctctccg
     1381 acaccgcgag accggcggcg tcgtcggccg cgctggtcac gcgcaagccg ctggagagtt
     1441 tctgaaagct gcccgcgagc gcgctctggc tgctggcaag gttcttctga gctgttaagg
     1501 atgcgctgtt ggtattgatc gttagtgcca ttggagactc cgtcctggag agtgactcgg
     1561 ctttcgccga ggccgggcgt cattgccctt cgtgtcattc cggaacgggt tctccgcgct
     1621 ggccttgagc acaaaacgaa gaccgccacg tgctccgctt cgcgccgtcg taacggcaga
     1681 gtgcgagccg agctccgtgg cggcgccatg aaggcccctt gcttacttga ggaggctgag
     1741 tgcgagctgc ggcagctggt ttgcctgcgc cagcaccgac gcgcccgcct gggacaagat
     1801 ctgattgcgg ctgagggccg cggtttcttc cgccacgtcg acgtcgcgga tgcgcgagtt
     1861 cgcggccgag aggttcaagc gcatggtttg aatcgacgac accgcgacct cgaggcggtt
     1921 catcgtcgcg ccgtatttgg cacggttggt cgacacggtc gtgagggcgg catcgatgcg
     1981 accgagggcg gcgagcgagt tcgtcgcggt ggcgcccgcg aggttgttcg tcgcgtcgag
     2041 gaggttcgtg aggccgaggc cgccgaacgt gagggtgatc tggtcggact gggtgttgtt
     2101 gaggccgatc tggaaggtga tcgagctcgc cgtcgacgac agtagctgct tgccgttgaa
     2161 cttcgccgat tgctgcatgc gctggatttc ttgcttcagc gccgtgaact cggtgtcgag
     2221 gtagccgcgg tcggtatcgg tcatgtcccc gttcgcgcct tgcatggcga gttcgcgcat
     2281 acggccgagg atgtcgctca tctcaccgag cacactctcc gcggtctgcg tcatcgagag
     2341 ggcgtcgttg ctgtttcgct ccgcgacggt gtacgagcga atctgcatct tcatgttctc
     2401 gctgatggcg agaccggccg cgtcgtcggc cgcggtgttg attcggaacc cgctcgagag
     2461 cctcgtgaag ttctgattga gcttggcttg ggttttcccg agcgctttct gggtttcgag
     2521 ggaggatacg ttggtttgga cgctaatcat ggctttcctt cggggggtag gggctcagtc
     2581 ctgtcgcagg cgcttggcaa gttttctgag ttcgtgctcg agcttccgag ctcaggcgtc
     2641 gagctgggcg ccgagccgcg cttcggaggc gagagccgtg taggcgttgt cttggttgtc
     2701 ctggttgact tcggtttgcc cggccatcac gccgtgcacc tcggcccagg accaggcgtc
     2761 gaggagcggc gcagacccca cgcgtttgcg caactcgagc tccgcggcgt cgagccgtga
     2821 gagtcgcgtc gtgacgctcg ggtcgccgcg cttggcggcg gtgagagcgg tctgagagag
     2881 tcgccgaatg cgccgcgccg tgtgacgcac ggatgcggcg aggtcggcct gattctcggc
     2941 ccacgcggcg atgcgcgccc gcgggagggc ttcggtctcg gcgtgcgcgt tcgcgacgat
     3001 gctctccggg gtgatatcga ggtcgggcag ggtcgagagc agcgcttcga gcgttacttc
     3061 atcgaagccc aaaatgcggg agccgccctc ggtcgcgttc acgagccgcg tctccggtga
     3121 ttcacggccg agcacgatgg cggcgctctc gagccagccg cggaccgccg agaatgcgac
     3181 gctcgacacg acgtggccat ctcctcccca cgcgaccgtc tcgttcaacg gttcactctc
     3241 ttggagaaag ccgccctcga ggcggtgcgt tgcgcggagc gtgtcgcacc aatcgagctt
     3301 gatgttgcgc ccgtccgcgc tgcgctcgac ccgcgagttc tcgtagggag tccccggcgc
     3361 gtaggctcgg ccgccagtgt aggcgaggtc ttgtcccacg aacacgatgg gggagcagcc
     3421 gagtcgctgc gcgagggcga agaccaacgt cgagacgctg ccgctcaccg cgagtcccgc
     3481 gtagccggtc agttggaaca gcggcaacga aagctgcgat atgccttccc acagcgcgag
     3541 cagcgggccc gcgccgaccc gcaagttctt ggggtgcgca gagagcgaga acgcgcgaat
     3601 cgaacggttc aggtagctca cctcggcgag caggtgcgag acgtcgatcg actccatgca
     3661 ggcgacgacc tgaggcgtga caccgagccg atctagagcg cgcgcgctcg agttgagcgc
     3721 aaagacgatc ccttttttct gggcttgtac caggtggtgc ccgttcttgc cgagcgacgg
     3781 gcccgcaccg acgataaacg ccggaacacc gcggtacttc ccaccgagtg ccagaaaccc
     3841 agggtgttcg gagagaagct cgacgttcgc gagcacgtcc tcgatccaat cgcgcgcacg
     3901 cagtcggtgt gtttcattgt tgacccggca gcgctgaacc agctcggcca ccgccgcccg
     3961 aagatcggct gacgcaccgg gaaaggcgtc ttcgtagccg ggggtgctga cgacgagcgc
     4021 ttggcgacgc tggccgaaga tggtgggcca gatttgcgtg aggtcatggg tagtgcagac
     4081 gatgtcgaag tcggagagcg cggtcggacc gagctcgagt acttgacgca agattccggg
     4141 gtccggctcg aagacgatga tgggctcgtt cgtcagctcc cgcagagcgc gcgcggtttc
     4201 gccgagcccg aggccgaaga cgacgatgag cgtatgctcg aggctcccgg cgtattcgag
     4261 ctccgcctcg ggcggagtct gtccgagcaa ggtgccctgg cggttgacga ccggcacgcc
     4321 gagctcgagc ccagcgagcc cgccggcgct gtgcgcgccg cggaccgcct ccaacgtgag
     4381 cgctgggagc gccatgtggc gcaggttctc ttcgaagaac tgctgcgaca tgtccgcggc
     4441 agtggggaag gcgcccgacg cgccatccgt gtcttcggat tgacgcccgc gaccgccatc
     4501 gagttgacga gcggcggctt ggctcatgga gacttgcccc acggaccaac tcccttcgga
     4561 agcggaagcg gaagctctgc aggatccgag atggtgttga ccgcctcgat gttcgcggct
     4621 tgaacggcgt cgtggagctc gccgcgtaaa atcgtggtgc gcgcgggcgc gacgatgcca
     4681 agtttgaccg agcctcgcgt cacttccgtg acgacgattt cgatgtcttg gccgatgacg
     4741 actcgttggc ccttgcggcg agctatgatg agcattcgct ggtgcgttgt gcatccagcg
     4801 tgccagtcgg gcgccgtagt cttcgcgaga attcgaacgc ggatcggcgc cgcacagctc
     4861 tacgagccgc tcgagggtac ggcactcgcc ggtcggctca gcgccagaga tcgccgaggt
     4921 cgaggacctg gcgtgacacc tggacggcct gctggagcga ttgagccagc gtggtcacct
     4981 tcgagtacgc ctcgaacgga tccacggcgc ccacgttcgc ttgctgctta ccgagatcga
     5041 ctccgagctg ctcgaggaca ccgtcgctcg cttgcagctt cgatgcggtg agcccgatgc
     5101 gggtgcgctc gcgggtcacc tgcgtggcgc cggcgtcgat ggcgtcgagg gcagcactcg
     5161 caccgctgcg gtcgttgttg tcgagagcgg tcgcgagcgc gtcgagatcc gcgaagacgt
     5221 ctcgtccacc agcggcggtg aacgccttcg cgccgctcgc gctcaccgcg acggggctgc
     5281 tgccgcccgt gtcgatcacc tgctcctgat cgtcgcctcg aaacgcgccg ttcgagtcga
     5341 acgcagctgc gtcggtcttg ctgccggcga agatgtagcc cttggtgccg cgcgtgttcg
     5401 cgagcttcaa gagctcggtt cgcaaaccgc gcacttgggt cgcgagcgcc ttgcgatcgt
     5461 cgacgccgag cgagtcgttc gagccctgca tcgccgcttc tttggcggac gccatgagct
     5521 cgttcacctg cgagagcgtt gactcggcga gctcagcgtc tccctgcgcg cgttggatcg
     5581 cttcgcgatg tccggcggag ttgctcagcg aggcgcgcag ccgcgcgagc tccgcagccg
     5641 cgaccggatc gtcggagggc ttctcgaggc ggaggccggt ggaggcctgt tgctgcgctt
     5701 gcgcgttctg cgtctgcaac cgggcgaggt tccgggtcac gccgaggtac ttcatcccat
     5761 cggtcacacg catggactac tcgctttcat cgggctcaaa cgcgctgaat cagatcttgg
     5821 agcaactgat ctgcggtggt caggagccgc gccgacgctt ggtacgcgcg ctggaacttg
     5881 gtgaggttca cgaactcttc gtcgagcgaa actccgctca tcgactcgtg catcgagtcg
     5941 acctgcgcgc tgatcgcctc gcgggcctcg agcatctgcg acgacgcgga gcggcggctc
     6001 gcgatgtcac cgacgatatc gccgtaagca tcgctcgcgg tgcgcgtgcc gcccgacgcg
     6061 atgggagtgc ggctcatcgc cgagaggagc gccgcgttat cggagccgcc aggcagcgtc
     6121 gcggccgagc tcgacgcggc gaccgcggcg gggttgcccg ctacgtcgtc gctgaggcga
     6181 atcgctcgcg cggcgccggt ggcggtcgcg gtgaggtcga agaggtgacg accagtcact
     6241 ccgtcgagac cgaaccccgc agcgtgctgg gtgttgatgg cggtgccgac gtcgtacgcg
     6301 aactgatcga gctgcttgcc gacggctacg aggtcgacgt cgcgcgcctc gcgaatgccg
     6361 gcgagcttgc ccccggtcaa gaatttcgtg acgtcctgag cggtgctacc gttgccgctc
     6421 gaggacaaca cctgcatcgc gcccgtcgcg tcgaggccga tgctcaagct gcggggcgcg
     6481 gcgccgtcga ccaacgttgc ccccgatacc tgcaccgtca acgaatcgcc cgactgcacc
     6541 gtgcggacgt cgaccatgcc ggagaggctc atcaggagct ggctgcgctt gtcgcggaga
     6601 tcggccgcgc tttgcccggc cgcctctgcc tgcgcgattt gctcgttgag cttggcgatc
     6661 tcttgcgcct tctggttgat ctcttggacg gtggcttgcg ccgcctggaa caaccccttt
     6721 tgcgcgtcgg cgatggcgtt ggcagtgtcg ttcactcgct tcgcgaactc gtccgcggcg
     6781 tcgagcacgc cgtctcgcgt cgtcggatcc gatgggttcg aagcgagcgc ggagaacgac
     6841 ttgaagacct ggtcgagaga cgagccgagc ccggtgcccg actggtcatt gaacagggcc
     6901 tcgatgttcg cgaggtcctt gtcgtgctcg gaggcggccg aggagagccc ggtcgtttcg
     6961 tactgccggc gctccaagaa cacgtccgtt acgcggcgaa tgcccgcggc gttgaccgaa
     7021 ccggcgtcgc cgagcgctcg cgtctcgagc aacgcctcgc gccgcacgta gcccggcgtg
     7081 ttgacgttcg agatgttctg gcccgtcaca ccgagcccgt acgactgcgc ggtgagaccg
     7141 tcgcgtgccg tgtagagcag ttgtgaaagc gagctggaca tcagaccctc acgttcagcc
     7201 ttaggccctc ggttgctgcg ggtttgcgtg cgttgggaag tccttcgaaa ggcgttccgc
     7261 tcacctgcga caggatcgtg cggacggcgt ctcgagcgtg gatcaagaga agctgattga
     7321 gcacggcgcg ctcgcgcagc ttctggaggc gcagacggtg gcgtggctcc ggccgctcgc
     7381 gctcggcgag cttctggagg cgc
//
LOCUS       DASTKS010001025         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_385198, whole genome shotgun sequence.
ACCESSION   DASTKS010001025 DASTKS010000000
VERSION     DASTKS010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385198"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFQ35_21685"
     CDS             <1..200
                     /locus_tag="VFQ35_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933338.1"
                     /translation="DDIPADAGWIWIVLQRSNLVKNVCLLGVCVHLTTHAPGRFSVDA
                     WLERRKHKRQREALPSTTSER"
     gene            227..1195
                     /locus_tag="VFQ35_21690"
     CDS             227..1195
                     /locus_tag="VFQ35_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET9933339.1"
                     /translation="MRAIRPKQAGGPDGLVLEEFEAPVAGPNQVLVRTEAIGVNYIDV
                     YQRTGLYKMPSPIPLGQEGAGVVEAVGPQVSEVKPGDRVAWASVPGSYATHVVLDAAR
                     TVPIPSGVDVRLAAALLLQGMTAHYLARSTFPLQSGHVALVHAAAGGVGLLLVQLAKA
                     AGARVIATVSTSEKAELARGAGADEVINYSEQDFLERTREYTSGRGVDVVYDSVGKDT
                     FAQSLEALAPRGYLVLYGQSSGPVEPFDPQRLAQRGSLFLTRPTLQHYTSTRAELLAR
                     AEELFRLVQTGRLRVRIDREFSLAQAADAHRALESRATRGKVLLIP"
     gene            1427..>2649
                     /locus_tag="VFQ35_21695"
     CDS             1427..>2649
                     /locus_tag="VFQ35_21695"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9933340.1"
                     /translation="MRKPSLLLQVADIIDSPQLRGGRARRCAPPPTFLLSSGAHSSPA
                     RLADGLRTLPAGSFVWTAWPPDLRHRVRRRLTLQVLTSTRAARCEPRRRATPPGFGLQ
                     GVQRDDPSQPPRSGEAAGPVLASRTSGMDPRVDVAVVGAGMAGLAAARALSKAGLAVC
                     VLEARDRVGGRVLTIRSPEHGAVELGAEFVHQDPEPTLRLAREAGVALIEIPDVHFEK
                     RGRELTLWEDAFEPMTRVLDELRDDEPDTSAAAFLAQREFDAETRRRFRQLVEGFEAA
                     PLGEVSIKSLVADAGASEESSAQRTVEGGYIELAEYLSERCRALGAKLRLRSPVTGVE
                     RRSEGVRIHVDGATLSARACVLTLPVGVLEHEGAERFALPDASKRLQQLGMGHAARVC
                     FVFEDRFRPDALPCE"
BASE COUNT          419 a          923 c          854 g          453 t
ORIGIN      
        1 ccgacgacat tccggccgac gcaggttgga tctggatcgt gctgcaacgg tcgaacctcg
       61 tgaagaacgt ctgtctgctc ggcgtttgcg tgcacctcac gacgcacgcg ccggggcgct
      121 tcagcgtcga cgcttggctc gagcgccgca agcacaagag gcagcgcgaa gccctgccca
      181 gcactacgtc cgagcgctag gattcggccc cgaaaggatg tgcgcgatgc gagcaatacg
      241 acccaagcaa gcaggcggac ccgacggcct cgtgctcgaa gagttcgagg cgcccgtagc
      301 cgggcccaat caggtactcg tgcgcaccga agcgatcggc gtcaactaca tcgacgtcta
      361 tcaacgcacg ggcctctaca agatgccaag cccgatcccc ctcggccaag agggcgctgg
      421 cgtcgtcgaa gcggtgggac ctcaggtgag tgaagtgaag ccaggtgacc gcgtcgcttg
      481 ggcgtcggtg cctggctcgt acgcgactca cgtggttctc gacgccgccc gcacggtccc
      541 gattccttca ggggtcgacg ttcgactcgc cgctgccctc ctcctccagg gcatgaccgc
      601 gcactatctc gcgcgctcga cgtttccgct ccaaagcggc cacgtcgcgt tggttcacgc
      661 agccgcgggc ggcgtcggcc tcctcctcgt gcagctcgcc aaggctgccg gcgcccgcgt
      721 gatcgcgacc gtttcgactt cggaaaaagc ggagcttgcg cgcggcgctg gtgcagacga
      781 agtcatcaac tactccgagc aagatttcct cgagcgcact cgtgagtaca cctccgggcg
      841 cggcgtggac gtcgtttacg actcggtcgg caaagacacg tttgcgcaaa gcctcgaagc
      901 gctcgcgccc cgaggctacc tcgtgctcta cggacaatcg agcggccccg tcgaaccgtt
      961 cgaccctcaa cgcctcgccc aacgcggctc gctgtttctc acccgcccca ccttgcagca
     1021 ctacacgagc acgcgtgcgg agctcctcgc gcgcgccgaa gagctctttc gcctcgttca
     1081 aaccgggcgc ttgcgtgtgc gcatcgatcg cgaattctcg ttggcccagg cagccgacgc
     1141 gcaccgcgcc ctcgagtcgc gcgccacgcg aggcaaagtt ctgctgatcc cgtagcctcg
     1201 ttgcatcgag ctcagcctgg gtcgcgcttt cgcacctgcc cacccccggc ctgctcggtg
     1261 cgtgctcgcg cgcgcgggca atccacgtcg ctttcaggct cgcgcgcgct gcgcgcgcca
     1321 cctgcgcggg ccttgcccct cccaaatgcc ggctcgctgt cgctcgcgcg gcatgggagg
     1381 ggcgccggct cgttcccgcc aagggcccgc gaagggtcaa gtcctaatgc gcaagccgag
     1441 tctcttgttg caggtcgcgg acatcatcga ttcaccgcag cttcgaggcg gacgggctcg
     1501 acgctgcgct cctccgccta ctttcctact ttcgtccggt gctcactcct cacccgcgcg
     1561 cctcgcggac gggctacgca cgcttcccgc gggtagcttc gtttggacag cctggccgcc
     1621 ggaccttcgg catcgagtgc gtcgccgcct caccttgcag gtgctcacca gcacacgcgc
     1681 agcccgctgc gagccccgca gaagagccac tccgcctggc tttggcttgc agggcgtgca
     1741 gcgcgacgat ccgtctcagc cacctcggtc gggtgaagcg gctggcccag tgcttgcctc
     1801 gcgcacgtcc ggaatggatc ctcgcgtgga cgtcgcagtg gtcggtgctg gcatggctgg
     1861 cctcgctgcg gcgcgggcgc tctcgaaggc cgggctcgcg gtgtgcgtgc tcgaagcgcg
     1921 cgatcgggtc gggggtcgcg tcctcacgat acgctcccca gaacacggcg cggtcgagct
     1981 tggcgccgag ttcgtgcatc aagaccctga accgacgctg aggttggcgc gcgaagcggg
     2041 ggtcgcactc atcgaaatcc cggatgttca tttcgagaag cgcggtcgag agctgacctt
     2101 gtgggaggac gcattcgaac cgatgacgcg cgtcctcgac gaactgcgag acgacgagcc
     2161 agatacgtca gccgcggcct tcttggcgca acgcgagttc gacgcggaaa ctcgccggcg
     2221 cttccgccaa ctcgtcgagg ggttcgaagc ggcgcccttg ggcgaagtga gtatcaaaag
     2281 cctcgtcgcg gacgccggcg cgagcgaaga atcgagcgcg caacggacgg tcgaaggcgg
     2341 ctacatcgaa ctcgccgaat atttgtcgga gcgttgccgg gccctcggag ccaagttgag
     2401 gcttcgctcc ccggtcacgg gcgtggaacg acggtcggaa ggagtccgta ttcacgtcga
     2461 cggcgcgacc ctgtcggcgc gcgcgtgtgt gctcactttg ccggtaggag ttctcgaaca
     2521 tgaaggggcg gagcggttcg cacttcccga tgcgagcaag cgactccagc agctcggcat
     2581 ggggcacgcc gcgcgcgtct gcttcgtctt cgaagatcgc ttccgccccg acgcgctccc
     2641 ctgcgaaga
//
LOCUS       DASTKS010001026         6567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_832014, whole genome shotgun sequence.
ACCESSION   DASTKS010001026 DASTKS010000000
VERSION     DASTKS010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832014"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..637
                     /locus_tag="VFQ35_21700"
     CDS             224..637
                     /locus_tag="VFQ35_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933341.1"
                     /translation="MRAKSFTAKRVRNVLIYVHSATAPDDDDWDQVLSFYGAATADAK
                     VCTLVYTEGGAPNAAQRARLNARLGPGIVRIAVLTPSAIARAAGTAVTWFRPHVRVFG
                     QHDFDRAMDHLEISPEGRQEIRDALQDMKRTLGLR"
     gene            complement(732..818)
                     /locus_tag="VFQ35_21705"
     tRNA            complement(732..818)
                     /locus_tag="VFQ35_21705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(782..784),aa:Ser,seq:tga)
     gene            1035..1661
                     /locus_tag="VFQ35_21710"
     CDS             1035..1661
                     /locus_tag="VFQ35_21710"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HET9933342.1"
                     /translation="MSRLNDSGALAMGAMAAAMFSVGMSLAVVACGSGSQPNDAAKAD
                     AKAHAVTPIASVGAAVAARTADALLNSQQTVSSVTSAAPTTSTQDVRTHSSEGVVVKR
                     FVVAQGIHEREPVTNDGPLTVNGKPIYAFAELANENSGATSVRITFERKGSSDRVGNV
                     TLSVPAKAGRYRTWGQTRFVRDAGTWDAVLWSESGKELGRTTFDVVGS"
     gene            complement(1906..2538)
                     /locus_tag="VFQ35_21715"
     CDS             complement(1906..2538)
                     /locus_tag="VFQ35_21715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933343.1"
                     /translation="MGFALLAVALGAAATLPGCALDVDECLPGEAVCDGDVARVCVTG
                     EYGGRRGHLWSSRDCGDAACVAVDGPSGVREAFCALSSDVDARCTEPEGASCDGEQLI
                     ACHVGRATSVTRCARGCVSLDDLPDYCREDPPEHARCVTGQGCELAGPAFTATAASAP
                     GANCSAAPFGPAADPTMHVYAYRCEDGLLTARQRCASVCLSEADCSTHCS"
     gene            complement(2779..3630)
                     /locus_tag="VFQ35_21720"
     CDS             complement(2779..3630)
                     /locus_tag="VFQ35_21720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET9933344.1"
                     /translation="MLRYVLCDVFTNQALTGNPLAVFTDARTLSSDTMQAIAREMNLS
                     ETVFVLPPSVGGHAKIRIFTPRAELKFAGHPTLGSAFVLGGPLQAETVRLETGVGIIP
                     VRLDRDGAKIAFGWMQQKVPSATPFTRAAELLEALGVERSLAPVVAYDNGAQHVYVVV
                     SSFEAVASLRPRLDRIAEIAEQTVNVCAISGARAKTRAFAPRHGIAEDPATGSAAGPL
                     TVHLAEHGLAAFGEQLIIEQGTEIQRPSELYAIAHGERGNVTSVDVGGSAVIIGRGEL
                     NIDARNQ"
     gene            complement(3703..4665)
                     /locus_tag="VFQ35_21725"
     CDS             complement(3703..4665)
                     /locus_tag="VFQ35_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933345.1"
                     /translation="MLRKTDERGFVGCALFVSAALVAACGGSAAPAAAPPSSDGATST
                     PAAASDAPSSDAPAAADSGSTAPANGTDSGSAPASKGPPSGEKLSLVALCNKGCDKMK
                     ATCSASSVDNCRMNCTQYEHPPAGCDDEIRAALECANKAEDTTCVNIAPEICSPKFRR
                     IVACANGGKTVETTDEAAQTPAGWERYSSKKNGFASMMPKGVAEGTDGGVPTSTVKQG
                     ELTYQVRVLPAPAERPTQKSMVKLALPILGKCSQKLKLYGMVDRPEKIFIRFDSHCPD
                     GTDWRGAFVITDKKMWMPFVTIPKGANGPKNEIDAFVYGFELEK"
     gene            complement(4700..>6567)
                     /locus_tag="VFQ35_21730"
     CDS             complement(4700..>6567)
                     /locus_tag="VFQ35_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933346.1"
                     /translation="QRAWSENWPGRARPWHRTTVSAKAAPPSWVASTCQGLALGVPWL
                     LGVWHANPNPHFASDFALVRSFGLLPVGFEGLVSAALSALLACLPLGGLTLRSAFVSA
                     LGLGLGGLFTYRIARGFIRRGGGRAAFEGPLSLLAALGATIGPSWILEGSVAGGHALV
                     AALALAALDVAEPFHWSPRRAVTLGALAALSILESRWTGIGVVLALALRRVLNGTELT
                     SAEGFALGIGFAAPLLLPVAAFFTFVASTSVRAGMALAATPSSLLPAAGSVERSVALS
                     AWLTEVGVIGLALSLVGVGFALRVPAERRVFAPLLVFLGLDLALRAANAEPTRHDPMW
                     GVRLVTLSALGIASVLALSKVLAWSSRARIVFARPAGILLFVYGVTLVLVSAEDASRA
                     AETRELGAASQYTDAALASLPPNTVLLVRSEPVLFRLLAAQATRGSRPDVLVVPFQLL
                     ERGGTRAAELAREPGLLPLVREMLLKGEPSEFALSALADARPTFVELGPFADERLAGH
                     LVPQPFFTAFASQPLARSDRQRELERNASGFARVVAALKDSPDGDPATRSVLSASLAE
                     RALLLASFGEREASAGLVGELRALDPRSPIALSLGQKLNQHGKGRIDVRALLAAR"
BASE COUNT         1166 a         2093 c         2325 g          983 t
ORIGIN      
        1 gttcatgact caccacctga ctgataaatc ctggagttcc gaacagtctg tgcgctcgaa
       61 gatgtctgca gcgttcgtgc cgcgcgaaaa ccgcctggaa atgtcgcctc tcgatcgcta
      121 gccagcgcac ctcgtgcacc cgatcggaac gtcgcgcaaa agcagcggcc cctacgggcg
      181 cgcgaccttg cgaacgcgcg attcgcttgg gtagcattgg acgatgaggg cgaagtcatt
      241 cactgcgaaa cgggttcgca acgtcctcat ctacgttcac agcgccactg cccccgacga
      301 cgacgactgg gaccaggtct tgagtttcta cggtgctgcg actgctgacg caaaggtctg
      361 cacactcgtt tacaccgaag gcggcgcgcc caacgccgct caaagagcgc gactgaatgc
      421 gcgtctgggc cccggcatcg ttcgcatcgc ggtcctgact ccctcggcca tcgcgcgcgc
      481 cgccggcacc gccgtgacct ggtttcgacc ccacgttcgc gtcttcggtc agcacgactt
      541 cgatcgcgcg atggatcacc tcgagatctc acccgaaggg cggcaagaga ttcgagacgc
      601 gctgcaagac atgaaacgaa ccctcgggct ccgctaaccg cgcgagcgat tcgagcccgc
      661 aagcgagcgc cctggcgcga gcggggagcc cgatccctgc tcctacgcaa gcggacctga
      721 agggtctgcg gcggaggagg agggattcga acccacggta cccttgcgag tacgccggtt
      781 ttcaaaaccg gtgccttaaa ccgctcggcc actcctccgg aggtgggcgc gttttggccc
      841 tgctgaacga gcccgtcaag gaggaggtga aaagggtgtg ggtgtcggga cacgcagggc
      901 gtctagccgg agacgcccct cgagcctgaa agccgccgat ttccgcgaaa accggcggtg
      961 tccagggtgg cccgggggtt gctgaagacc cctcgtgctc gcgccgaaga gcggcgcgcg
     1021 cgaaaggatt gagcatgtct cggttgaatg attcgggggc gctggcgatg ggagcaatgg
     1081 cggcagcgat gttctcggtg gggatgtcct tggcggtcgt agcgtgcggc tcggggtctc
     1141 aaccgaacga cgccgcaaag gctgacgcca aagcgcacgc agtgacaccg atcgcgtcgg
     1201 tgggcgccgc ggtcgccgcg cgcactgcgg acgcgctgct gaactcacag caaacggttt
     1261 cgtccgttac ttcggcggcg ccgacaacga gcacacagga cgtccgcacc cactcgagtg
     1321 aaggcgtcgt ggtgaagcgc ttcgtggtcg cgcaaggtat ccacgagcgc gagcctgtga
     1381 cgaacgacgg tccgttgact gtaaacggaa agccgattta cgcgttcgcg gagctcgcga
     1441 acgaaaactc cggagcgacg agcgtgcgca tcactttcga acggaagggt tcgagtgatc
     1501 gggtgggcaa cgtaacgctc tccgttcccg cgaaggccgg acgctaccgc acttgggggc
     1561 agactcgatt cgtgcgcgat gccgggacgt gggacgccgt cttgtggagc gaaagcggta
     1621 aagagctcgg tcgcacgacg ttcgatgtcg tcggctcctg aaacgaactt ctcacggggt
     1681 gatcgcagag ggacgaggag tgcgcgcgag ggggtggcgg gggtgccgcg ggtcggtggc
     1741 gcgagggcgg gtggcccggg cgggtggcgg gaaggcgcgg gtgggcgggc ggcgcgagtg
     1801 gcccgggcgg gtggcgggag ggcgcgggtg ggcgggcggc gcgagggcgg gtggcccgga
     1861 cgggtgggcg gggagcagag cgcggggagc aggccgaggg gggatttacg aacagtgggt
     1921 agagcagtcg gcttcgctga ggcagacgga tgcgcagcgt tggcgtgcgg tgagcaggcc
     1981 gtcttcgcag cggtaggcgt agacgtgcat ggtgggatct gcggcaggac cgaaaggagc
     2041 cgcggaacag ttggcgcccg gcgcggaggc agcagtcgct gtgaaagcgg ggccagcgag
     2101 ttcgcagccc tgcccggtga cgcagcgcgc gtgctcggga ggatcttcgc gacagtagtc
     2161 ggggagatcg tcgaggctga cgcagccgcg agcgcacctc gttacggaag tggcgcgacc
     2221 gacgtggcaa gcgatgagct gctcgccgtc gcacgaggcg ccttcgggtt cggtgcagcg
     2281 cgcgtcgacg tcgctcgaga gtgcacagaa ggcttcgcgg actccgctcg gaccgtcgac
     2341 agcgacacac gccgcgtcgc cgcaatctcg cgagctccaa agatggcccc ggcgcccgcc
     2401 gtactcaccc gtgacgcaca cgcgagcgac gtcgccgtcg cagacggcct cccccggaag
     2461 gcattcgtcg acgtcgagtg cgcagcccgg aagtgttgcc gctgcgccaa gcgccaccgc
     2521 gaggagcgcg aatcccactc ggacaggtcg tcgaaaaagg gaaacagcgt gaaccccgga
     2581 ccacacggcc gtgctggaaa aacgctggtt tcgtcggaaa ttgcgggtgg cggacatgct
     2641 tgaaccagta cttcagcacg tcgcgtgcca gccctcgcgc cgccgaagta gacgccgacg
     2701 cacgggcgct cactggtggc gagcctcgat gttcagctcg ccgcgacgac cttcaccgcg
     2761 aagccgccga cgtcctcgtc attggttgcg agcgtcgatg ttgagctcgc cgcgtccgat
     2821 gatgaccgca gagccaccaa cgtcgacgct ggtcacgttg ccgcgctctc cgtgcgcgat
     2881 ggcgtagagc tccgagggtc gttggatttc ggtgccttgc tcgatgatga gttgttcacc
     2941 aaaggcggcg agcccatgtt cggcgaggtg caccgtgagg gggcccgccg ctgagcccgt
     3001 cgcaggatct tcggcgatgc cgtgacgcgg cgcgaaggcg cgcgttttgg cgcgcgcgcc
     3061 cgaaatcgcg cagacgttca ccgtttgctc ggcgatctcg gcgatgcgat cgagacgcgg
     3121 tctcaaggat gcaaccgctt cgaacgaact cacgacgacg tagacgtgct gcgcgccgtt
     3181 gtcgtacgcg acgaccggcg cgagcgagcg ttcgaccccg agcgcctcca agagctcggc
     3241 cgcgcgcgtg aagggcgtcg ccgaaggaac cttctgctgc atccatccaa acgcgatttt
     3301 cgcgccatcg cgatcgagcc gcaccggaat gatcccgacg ccggtttcga ggcgcaccgt
     3361 ttccgcttgg agcggaccac cgagcacgaa cgcgctgccg agcgtcggat ggcctgcgaa
     3421 tttgagctcg gcgcgcggcg tgaagatgcg gattttcgcg tgcccgccga cactgggtgg
     3481 gagcacgaac acggtctcgg aaaggttcat ctcgcgcgcg atggcctgca tcgtgtcgct
     3541 cgagagcgtg cgcgcgtcgg tgaagacagc gagcggattg cccgtgaggg cttggttcgt
     3601 gaagacgtcg caaaggacgt aacggagcat tcgcacggtt gacctcgttt tacggcgcgg
     3661 ggttctggca agtggcgccg ttcgcgggcg cgcggcgcag gatcacttct cgagctcgaa
     3721 accgtacacg aaagcgtcga tctcgttctt cggtccgttc gcgcccttgg ggatcgtgac
     3781 gaacggcatc cacattttct tgtccgtgat gacgaaggcg ccgcgccagt cggtgccgtc
     3841 agggcagtgg gagtcgaacc ggatgaagat cttctccggg cgatcgacca tgccgtaaag
     3901 cttgagcttt tggctgcact tgccgaggat gggcagcgcc agtttcacca tgcttttttg
     3961 ggtggggcgt tccgctggcg ccggcagcac gcgcacttga taggtgagct cgccctgctt
     4021 cacggtggag gtcggaacgc cgccgtcggt tccttcggcc acgcccttcg gcatcatgct
     4081 ggcgaagccg ttcttcttcg aagagtagcg ctcccagccg gcgggggttt gcgcggcttc
     4141 gtcggtggtc tcgacggtct tgccgccgtt tgcgcaagcg acgatgcgcc ggaacttggg
     4201 agagcagatc tcgggggcga tgttgacgca ggtggtgtct tcggccttgt tggcgcactc
     4261 gagcgcggca cgaatctcgt cgtcgcagcc ggccggcggg tgctcgtact gcgtgcagtt
     4321 catgcggcag ttgtcgaccg agctcgccga gcacgtagcc ttcatcttgt cgcagccctt
     4381 gttgcagagc gcgacgagag agagcttttc accgctcggc ggacccttgg acgcgggcgc
     4441 cgaaccggaa tcggtgccgt tggcgggcgc ggtcgagccg ctgtccgcgg cggccggggc
     4501 atcgctcgaa ggagcgtcgc tcgccgccgc cggagtgctc gtcgctccgt cggaggatgg
     4561 tggcgccgcc gcgggcgcag ccgacccgcc gcacgcagcg acgagcgccg cggatacgaa
     4621 cagcgcacac ccaacgaagc ctcgctcatc agtctttcgc agcatcgtca tggaccttaa
     4681 cctactctct cgaatgcggt cagcgagcgg cgagcaaggc gcgcacgtcg atgcgtcctt
     4741 tgccgtgctg gttgagtttt tgtcccaaag acagagctat tggggagcgc gggtcgagcg
     4801 cgcgaagctc accgacgagc cccgcgctgg cctcgcgctc gccgaacgaa gcgagcaaaa
     4861 gagcgcgctc ggcgagcgag gccgagagaa cgctccgagt cgcgggatcc ccgtcggggc
     4921 tgtccttcag cgccgcgacg acgcgcgcaa agccgctcgc gttgcgctcg agctcacgct
     4981 gccgatccga gcgcgccagc ggctgcgagg caaacgccgt gaagaagggt tgcggcacca
     5041 agtgccccgc gaggcgctcg tccgcgaacg gaccgagctc gacgaaggtc gggcgcgcgt
     5101 cggcgagcgc tgacaacgca aactcgctgg gctcaccctt caggagcatc tcgcggacga
     5161 gcggcaagag ccccggctcg cgagcgagct cggcggcgcg cgttccgcca cgctcgagca
     5221 gctgaaacgg gacgacgagc acgtcggggc ggctgccgcg cgttgcttgg gcggctagca
     5281 agcgaaacaa cactggctcg gagcgcacga ggagcaccgt gttcggcggc aagctcgcga
     5341 gcgccgcatc cgtgtactgg ctcgcggcgc cgagctcccg cgtttcggcg gcgcgcgaag
     5401 cgtcctcggc gctcacgagg acgagcgtca caccgtagac gaagaggagg atgccagcgg
     5461 gccgcgcaaa cacgatgcgc gcgcgagagg accaagcgag caccttggag agcgcgagca
     5521 cggaagcgat cccgagggcg gacagcgtta ccaggcgtac gccccacatc gggtcgtggc
     5581 gcgtcggctc cgcgttcgcc gcgcgcagcg cgaggtccaa ccccaaaaag acgagcaacg
     5641 gcgcgaatac ccggcgctcg gcagggacgc gtagcgcgaa ccccacccca accaaggaca
     5701 atgccaaacc aatgacgccg acctccgtaa gccacgccga gagcgccacg ctgcgttcga
     5761 ccgatcccgc cgccggcagc aggctcgacg gggtcgccgc aagcgccatt cctgcgcgaa
     5821 ccgaagtcga ggcgacgaag gtgaagaacg ccgcgaccgg caggagcaac ggggccgcga
     5881 acccgatccc gagcgcgaag ccttcggcgg aggtcagctc cgtgccgttc agtacgcgcc
     5941 gcaacgcgag cgccaagacc acgccgatac ccgtccagcg cgactcgaga atgctgagcg
     6001 ctgcgagcgc gcccaacgtc acggcccggc gcggcgacca gtgaaacggc tcggcaacgt
     6061 cgagcgccgc gagcgctagt gccgcgacca gagcgtgtcc gccggctacg gagccttcca
     6121 ggatccagct cggaccaatg gtcgcgccga gcgccgcgag cagcgaaagc ggaccttcga
     6181 acgccgcgcg gccaccgcct cggcgaatga agcctcgcgc gattcgataa gtgaagagcc
     6241 caccgagccc gaggccaaga gcgctgacga aagcgcttcg cagagtgagc ccgccgagcg
     6301 gcagacaggc gagcaacgcc gagagcgcgg ccgaaacgag gccctcgaag ccgaccggga
     6361 gaaggccaaa gcttcgcacc agcgcgaagt cgctcgcaaa gtgggggttt gggttcgcat
     6421 gccagacgcc caggagccaa ggcacgccaa gggcgaggcc ctgacacgtc gaggcgaccc
     6481 aagaaggtgg cgcagctttc gccgacaccg ttgttcggtg ccacggccgc gcccggccgg
     6541 gccagttttc ggaccaggct ctttggg
//
LOCUS       DASTKS010001027         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1504313, whole genome shotgun sequence.
ACCESSION   DASTKS010001027 DASTKS010000000
VERSION     DASTKS010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504313"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFQ35_21735"
     CDS             <1..235
                     /locus_tag="VFQ35_21735"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET9933347.1"
                     /translation="LCKSEEGRREVQAAIDDYAPYLPKFFGAANSKNNEAYRKFGLKE
                     RTNEQMREDFMARARTVVESLGLRLPELEPAQA"
     gene            253..1296
                     /gene="gtdA"
                     /locus_tag="VFQ35_21740"
     CDS             253..1296
                     /gene="gtdA"
                     /locus_tag="VFQ35_21740"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HET9933348.1"
                     /translation="MTATPTSTTSERADFYRRIDRENLTPLWESLAQLVPPEPRSPCV
                     PAIWRYGTVRPHLMEAGRLISAQQAERRVLVMENPGLRGASSITRSLYAGLQLILPGE
                     VAHSHRHTQSALRFVVEGSGAYTAVNGERAVMHPGDFIITPSWTYHDHGNPADEPVVW
                     LDGLDIPMVAFFDAGFAERYAEPRQPLTRPDGDALRRYGENLLPLEYVTDSRSTPLLS
                     YPYARTRSALEALRSHAELDPSHGLKMQYANPATGGYPMPTIAAYIQMLPKGFQGRGY
                     KSTDGTVYSVVEGRGRTRVADSVFEWEPRDVFVAPSWARVAHEAFEDAVLFSFSDRPV
                     QKVLGIWREEVER"
     gene            1293..1796
                     /locus_tag="VFQ35_21745"
     CDS             1293..1796
                     /locus_tag="VFQ35_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933349.1"
                     /translation="MSDWFFMLGEPLSAAELEHVRAYLKGLDLDSHTPVESTRDFEEA
                     RRIISHPDWDRRWWDAEQRERERLRAKLAGLRESDRLSLELSQSMEQSVSAQHGAAAV
                     EAGRLGCTDQALIRAAAGALGEALYLGKLAELSGEPSSHPFVLKQALFTGGHWPLGIV
                     AGRYYVF"
     gene            1822..2529
                     /locus_tag="VFQ35_21750"
     CDS             1822..2529
                     /locus_tag="VFQ35_21750"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9933350.1"
                     /translation="MNYAFALPAVPSVPIAGTESHFPVRRIYCVGRNYAEHAREMGFE
                     AEREAPFFFTKPADAIVLDGASIEYALATRNLHHEIELVVAIGKTGVDISREAALDHV
                     YGYAVGIDMTRRDLQLAARDKGRPWDVGKGFDESAPIGTIRRAAEIGHVNEGAIRLSV
                     NGEVRQSADLKQLISPVPDLVVELSKLFVLQPGDLIYTGTPAGVGPVKPGDELRGSIE
                     HVGEISIRIRARAGLER"
     gene            2526..>3086
                     /gene="maiA"
                     /locus_tag="VFQ35_21755"
     CDS             2526..>3086
                     /gene="maiA"
                     /locus_tag="VFQ35_21755"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET9933351.1"
                     /translation="MKLYNYFRSSASYRVRIALNLKGVAYDYHSVHLNRNGGEQFSPA
                     FREVNPQSLVPVLEDGEQRLAQSLAIIEYLDELYPDPPLLPRTPIERARVRGLALTVA
                     CEIHPLNNLRVLNYLSQELGVSADARTRWYRHWVEAGLSAFEARIAEGPSGRFCHGDR
                     PGLADCCLVPQLFNARRFDCDLSPYPM"
BASE COUNT          564 a         1021 c          963 g          538 t
ORIGIN      
        1 gctctgcaag agcgaagaag ggcgacgcga ggtccaggcc gccatcgacg actacgcccc
       61 ctatttgccg aagttcttcg gggccgcgaa ctcgaagaac aacgaagctt accggaagtt
      121 cggcttgaag gagcgcacca acgagcagat gcgcgaggac ttcatggctc gagcgcgcac
      181 cgtcgtcgag tcgctcgggc ttcgcttacc ggagctcgaa ccagcacagg cctgaagcac
      241 gagggcatca ccgtgaccgc cacgcccacg tcgaccacca gcgagcgcgc cgatttctac
      301 cggcgcatcg accgcgagaa cctgacgccg ctttgggaat cgctcgcgca gctcgtgccg
      361 cccgagccgc gctctccctg cgtgcccgcc atctggcgct acgggaccgt acggccgcat
      421 ttgatggagg cagggcggct catcagcgcg cagcaagcgg aacgccgtgt gctcgtgatg
      481 gagaaccctg ggcttcgcgg cgcatcgtcc atcacgcgct ccctctacgc cgggctccag
      541 ctcattttgc cgggtgaagt ggcgcacagt caccggcaca cccagtctgc gctccgcttc
      601 gtggtcgagg gcagcggcgc ctataccgcc gtgaacggtg aacgcgcggt gatgcaccct
      661 ggcgatttca tcatcacccc gtcgtggacc tatcacgacc acgggaaccc ggcggacgag
      721 cccgtcgtct ggctcgacgg actcgacatt ccgatggttg cgttcttcga cgccggcttc
      781 gccgagcgct acgcggagcc acgccaaccc ttgacccggc cggacggcga cgcactgagg
      841 cgctacggag aaaatctcct acccctcgag tacgtcaccg actctcgctc gaccccgctg
      901 ctttcgtatc cctacgcgcg cacccgttcc gccctcgagg cgctcaggtc gcacgccgag
      961 ctcgacccct cccatgggct caagatgcag tacgcaaacc cagcgacggg cggctatccc
     1021 atgccgacca tcgcggcgta cattcaaatg ctccccaaag gttttcaggg gagaggctac
     1081 aagtcgacgg acggaaccgt ctatagcgtg gtcgagggcc gcggccgaac tcgtgtggcg
     1141 gattcggtat tcgagtggga gccgagagac gtcttcgtcg ctccctcctg ggcccgagtc
     1201 gcgcacgagg cgttcgaaga tgccgtgttg ttcagcttct cggatcgccc ggtgcaaaag
     1261 gtgctcggga tctggcggga ggaagtcgag cggtgagcga ttggttcttc atgctcggcg
     1321 agcctctttc agctgccgag ctcgagcacg tgcgagcgta cttgaaaggg ctcgacctcg
     1381 actcgcacac gccggtcgaa agcactcggg acttcgagga ggcccgtcgc atcatttcgc
     1441 atccggattg ggatcggcgc tggtgggatg cggaacagcg cgagcgcgaa cgacttcgag
     1501 ccaagctcgc cgggctgcgc gagagcgacc gcctctcgct agagctctcc cagagcatgg
     1561 agcaatccgt gtccgcgcaa cacggagccg cggcggtgga ggccggacgc ctcggctgca
     1621 ccgaccaggc attgatccga gcggccgctg gtgcgctcgg cgaggctctc tatttgggga
     1681 agctcgccga gctctccggt gaaccttctt cccacccatt cgttttgaag caggcgctgt
     1741 tcacgggcgg gcactggccg ctcgggatag tggccgggcg ttactacgtc ttctgaagac
     1801 gtcgaccagt aaaggactgt catgaactac gcattcgcct tgcccgccgt gccgagcgtg
     1861 cccatcgccg gaaccgaatc gcatttcccc gtgcgtcgca tctactgcgt gggacgcaac
     1921 tatgctgagc acgcgcgcga gatggggttc gaagccgaac gagaagcccc cttcttcttt
     1981 accaaacccg ctgacgcaat cgtcctcgac ggcgcgagca tcgagtatgc gctcgccact
     2041 cgtaatctgc accacgagat cgaactcgtc gtcgcgatcg gcaaaacggg cgtcgacatc
     2101 tcgcgtgaag cggccctcga tcacgtctac ggttatgcgg tcggcatcga catgacgcgc
     2161 cgagatttgc agcttgccgc gcgcgacaag ggacggccat gggacgtcgg aaaaggcttc
     2221 gacgagtcgg cgccgattgg gaccatccgg cgcgcggcgg aaatcgggca cgtgaacgaa
     2281 ggggccattc ggctttcggt caacggagaa gtcaggcaga gcgccgattt aaagcaacta
     2341 atctcccccg tccctgatct cgtcgtcgag ctctccaagc tcttcgtcct gcagccgggc
     2401 gacctgattt atactggcac accggcgggt gtgggcccgg tcaaaccggg agacgagctg
     2461 agaggcagca tcgagcacgt cggggaaatc tcgatccgca tcagggctcg cgcgggactc
     2521 gagcgatgaa gctctacaac tatttccgaa gctccgcgtc ttatcgagtc cgcatcgccc
     2581 tcaacctgaa gggcgtagcg tacgactacc actcggtgca cctgaaccgg aacggaggcg
     2641 agcagttttc gccggccttt cgcgaggtca acccgcagtc gctcgtcccg gtgctggaag
     2701 atggcgagca gcggctggcc caatcgctcg ccatcatcga gtacttggac gaactttacc
     2761 ccgacccacc gctgctgccg cgcacgccga tcgagcgcgc tcgcgttcgc ggactcgccc
     2821 taacggtggc atgcgagatt catcccctga acaacctacg cgttctgaac tatttgagcc
     2881 aggagctcgg ggtgagcgcg gacgcgagga cccggtggta ccggcactgg gtagaagcgg
     2941 ggctcagcgc gttcgaagcc cgaatcgcgg aagggccgag tggtcgcttc tgccacggcg
     3001 accgcccggg cctcgcggac tgttgcttgg ttccacagct cttcaacgca cgacgcttcg
     3061 actgcgatct ctccccttac cccatg
//
LOCUS       DASTKS010001028         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_163066, whole genome shotgun sequence.
ACCESSION   DASTKS010001028 DASTKS010000000
VERSION     DASTKS010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163066"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ35_21760"
     CDS             <1..205
                     /locus_tag="VFQ35_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933352.1"
                     /translation="SQYQGRIIGDYQKFSHEPVLFFQAQLHRDFRALYEAQSGCLQSR
                     GRSSTTDASAAAEPTSPRGSRQN"
     gene            679..1983
                     /locus_tag="VFQ35_21765"
     CDS             679..1983
                     /locus_tag="VFQ35_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933353.1"
                     /translation="MELRFATVPRALIVSALLGASLALSGCGADANAEPPLPEPSTGE
                     KSASVSGLSGVEGQSCAALAETAVKSFRPIDVIFLIDNSGSMSDEIAAVERSINRDFA
                     QIMEASGLDYRVIMVSRYGTPGSSVGQSENPICVGAPLSASDCDESEKAPVNTERFFH
                     YSANVQSKDGWCVLLGGYDQPDEYGDVARPGWTANAPKGWRQFLRPEAFKTFIAIGDD
                     EVDCRVGARSFSDHSTASGGEVAAQAFDSALLGLSPEQFGTARGRNYIWHSIVGMRAY
                     SPTAATWPASAPVDTRLCGSGAQAPGTGYQALSRLTGGMRSPSCDAEHLDTVFNTVAH
                     GIVGAARIDCRWQIPSPPAGQRFDQRLVNVRYTPSADRSTQNIAYVGGASQCRFGGGW
                     YYDDPASPRNIVVCPSTCSTLQQDSAARVDVLFGCATDAVVR"
     gene            2053..2571
                     /locus_tag="VFQ35_21770"
     CDS             2053..2571
                     /locus_tag="VFQ35_21770"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9933354.1"
                     /translation="MRLGREVLETDLYASLGVDPSANFQQIRARYWRLARESHPDIHP
                     DDPAALRRMQELNLAARVLLDPAQRRLYDVGRGRQTTSWYEKVSVGSSEWHWPEPSAV
                     DSTASAANSEFRQRRERTALWLQDCFFAMSNERRLSIAALCLALGWGLISYAHPSNAF
                     WLPRHGVNAAFR"
BASE COUNT          453 a          886 c          937 g          471 t
ORIGIN      
        1 ctcgcaatat caaggccgca tcatcggcga ctatcagaag ttctcgcacg aaccggtgtt
       61 gttcttccaa gcacagctgc accgcgattt ccgcgcgttg tacgaggcgc agtctggatg
      121 cctgcaaagc cgcggtcgct cgtcgacgac tgacgcgagc gcggccgccg aaccgacgtc
      181 gcccaggggc tcacgccaga actgagcgac cggtcacgct ggccctgcgg tagtcaccgg
      241 aacagcgcgg gcctccgctg gcaacgagcg caatcggacc gactccgatg tagccagcgg
      301 gggccgcggt gtgaaagcgt ggcagtgcac tgtgagcccg agtgcatgcc gagcgaggcg
      361 tagggccgag cggggcgcag tggcgagcgg ggcgcagtgc cgagcgaggc gcagtgccga
      421 gcggggcgca gtgccgagtg ccgagcgggg cgtggtgccg agcggggcgt ggtgccgggc
      481 ggggcgtggt gccgggcggg gcgtggtgtg agcttctgac ggacgcagcc ctctgggctc
      541 gtcacatatc gggcaaggca cacccccgtt cacccggcgc gatgattggc gaatgggtgc
      601 gggtccttct cacaaatcgt cgcgcggtca acgctctgac gctaacggcg gggatggttt
      661 tcgccgctcg atagtgtcgt ggagttacgc ttcgcgacgg ttccgcgcgc gttgatagtg
      721 agcgcgctgc tcggcgcttc gcttgcgctg tccggctgcg gcgctgacgc gaacgctgag
      781 ccgcccttgc ccgaaccctc tacgggagaa aagtcggcga gcgtttcggg gctttcaggg
      841 gtggaaggac aatcctgcgc cgcgctcgcc gagaccgcgg tgaagtcgtt ccgtcccatc
      901 gacgtcatct tcctgatcga caattcgggc agcatgagcg acgagatcgc ggccgtcgag
      961 cgcagcatca accgcgactt cgctcaaatc atggaggcga gcgggctcga ctaccgcgtg
     1021 atcatggtgt cgcgctacgg tacgccgggc agcagcgtcg gtcagagcga gaatcccatt
     1081 tgcgtcggtg cgccgctctc ggcctccgat tgcgacgaat ccgaaaaagc gcccgtcaac
     1141 accgagcgct tctttcacta cagcgcgaac gttcaaagca aggacggctg gtgcgtgctg
     1201 cttggcggct acgaccagcc cgacgagtat ggcgacgttg cacggccggg atggaccgcc
     1261 aacgcaccca agggttggcg tcagttcttg cggcccgagg ccttcaagac gttcatcgca
     1321 atcggcgacg acgaggtcga ttgccgcgtc ggtgcgcgct cgttcagcga tcactcgact
     1381 gcttccggcg gcgaggtcgc ggcgcaggcg ttcgactcgg cgctgctcgg gttgagccca
     1441 gagcagttcg ggaccgcgcg cggaagaaac tacatttggc acagcatcgt cggcatgcgc
     1501 gcttactcgc ccacggccgc gacgtggccg gcgagtgcgc cggtcgacac gcggttgtgt
     1561 ggtagcggcg ctcaggctcc tggcactggg taccaggcgc tgagcaggct taccggtggc
     1621 atgcgctcgc ctagctgcga cgccgagcac ctcgacaccg ttttcaatac ggtagcgcac
     1681 ggcatcgtcg gcgcggcccg catcgattgt cgttggcaga tcccttcgcc gcccgccggg
     1741 cagcgcttcg accagcggct cgtcaacgtg cgctacacgc cgagcgccga ccggagtacc
     1801 cagaacatcg cgtacgtggg cggtgcatcg cagtgcaggt ttgggggcgg ctggtactac
     1861 gacgaccctg cttccccacg gaatattgtg gtgtgcccgt cgacgtgttc gacgctccag
     1921 caggattcag cggcccgcgt cgacgtcctc ttcggctgcg cgaccgacgc cgtggtgcgc
     1981 tgaaaagaat cgcgagccct ggaacttcga gggctgccgc gtgtcgaacc tgaaacccgg
     2041 ggagactttc cgatgcgact tggccgtgag gtactcgaga ccgaccttta tgcgtcgctg
     2101 ggcgtcgacc caagcgcaaa ctttcagcaa atccgcgcgc ggtactggcg cttggcccgc
     2161 gaaagccacc ccgacattca cccggacgat cccgccgcgc tccgccgcat gcaggagctg
     2221 aacctcgccg cgcgagtcct gctcgacccg gcgcaacgcc gcctctacga cgtcgggcgc
     2281 ggtcggcaga cgaccagctg gtacgagaag gtgagcgttg ggtccagcga gtggcattgg
     2341 ccggagccga gcgccgtcga ttccacggca agcgcagcga actctgagtt tcgccaacgc
     2401 cgcgagcgca ccgctctctg gctacaggac tgcttttttg ccatgagcaa cgagcggcgt
     2461 ttgagcatcg ccgcgctctg cttggcgctc ggatgggggc tcatctctta cgcgcatccg
     2521 agcaatgcgt tttggctgcc tcggcacggc gtgaacgctg cgttccgctg accgagctgc
     2581 ggagtgcgcc gcgcgctttg cgagcgccca gatgagcgct cgcggcggct aacgacgatt
     2641 ctcgagctcg tacttcatga cgtaacagaa cggcacggcc ttgctgcctt cgagcagtgg
     2701 ccggtacctc caactcgaaa cggcgcggga cacttcctga tcgatgc
//
LOCUS       DASTKS010001029         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_609261, whole genome shotgun sequence.
ACCESSION   DASTKS010001029 DASTKS010000000
VERSION     DASTKS010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609261"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFQ35_21775"
     CDS             complement(<1..1232)
                     /locus_tag="VFQ35_21775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933355.1"
                     /translation="MSDPSDRPTPHRLADFEIVRRLGAGGMAEVFLGKKRGAEDTFKL
                     LVLKRVLPAYGNSRRFRTMFAEEAQLATRLNHPNIVQVYDFQDYGEEGQLLSMEYVEG
                     PDLRRLMKAAQARGQRIPPYVAAYIIAEVAKGLHYAHERKDEAGAPLDIVHRDVSPQN
                     ILLSYDGAVKIADFGIASANLFREEPGVLKGKTAYMSPEQARGERVTRRTDIYSLGVV
                     FHELLSGRPLHGEAEGQELLEAVRAGQVEPPSTFVRELPPELEEIVMRALARDPAQRF
                     QTAREMAAGISRALIQRQELVDSHVLEVVIAQLITREHTSPGPQRGSEIQGDSMYGPD
                     SIGQQSVAASRVDAGAEEAAAHDEQTGPGRLGREGLRERAGREVRHVALLKLRLHGVE
                     ELVATAGVAPATRVLEQLR"
     gene            1485..2612
                     /locus_tag="VFQ35_21780"
     CDS             1485..2612
                     /locus_tag="VFQ35_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933356.1"
                     /translation="MRWCVALALISTLLSSNALAAPKGKKGKNTKKDDKSGASMETTG
                     ADPVRTETSDPGPYAPKGMTGAMGEEAEHTEEVKQVVKAKPRDKALLFADVVVGWGNS
                     PKPGPNRANASGGRDDQLNDAIAGSLILGGRFDLSPKFSLGFRLPIGSSLVDRGGALG
                     KQSHVAVGAIEVLGEYRHYLSPLTSLPILFGLGLPTGTDDPDPSTGETSNRTNTIVDS
                     ARGWRDGELFWSKRVPLILGIGIRHENRSLELRAATKVVAGFRIAGDLANANYYGAPR
                     SELQNNFVALRSVTDVGLSYSFTEKFWAGADIWAAANIIEPIHFKSTAEPPFLFQFVA
                     EPMVGLRFGKIRPNLGFIIPLNGRLADGGMYGGQLRVDVAL"
     gene            2682..>3561
                     /locus_tag="VFQ35_21785"
     CDS             2682..>3561
                     /locus_tag="VFQ35_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933357.1"
                     /translation="MRARRLRSLPRFGVLALALSANLGCASGPRPHPAKPTATLVTGP
                     VQAAPVDEQNFAESTYQLLLDGEPSRERVGKLIGVVRHQLARSEARFEGGSSDAGLRA
                     LLGAFMLVRAGEHRPELLNGGERALGNAATEVARLGREGYSVALYTMLASALPASPAR
                     NEVEAHLRAIQDFSRSTQGGGPVQAAGIGARVAVQRALLEATDESLTAARDAMVTWIR
                     RALELNATEQPIRSNLDRDESIEAYRGLHSGAIALTALYLRHGDPRGALSAIDHAELQ
                     RLLSPELRDHLERIADE"
BASE COUNT          599 a         1190 c         1152 g          620 t
ORIGIN      
        1 cgcagctgct ccaacacgcg cgtggcggga gcgacgccgg ccgtcgccac gagctcttcg
       61 acgccgtgca atcgcaactt gaggagcgca acgtgacgca cttcgcgccc cgcgcgctcg
      121 cgcaaaccct cgcgaccgag ccgccccggg cccgtttgct cgtcgtgcgc ggccgcctct
      181 tctgcccccg cgtcgacccg cgacgccgcg accgattgct gcccaatcga gtcggggccg
      241 tacatgctgt cgccctgaat ctcggagccg cgctgaggac cgggcgatgt gtgctcccgc
      301 gtgatgagct gcgcgatgac gacctcgagc acgtgcgaat cgacgagctc ttggcgttga
      361 atcaacgcgc ggctgatgcc agccgccatc tcgcgcgcag tctgaaaacg ctgcgcggga
      421 tcgcgcgcca acgcgcgcat cacgatctct tcgagctcgg gcggtagctc acgcacgaac
      481 gtgctcggcg gttcgacctg acccgcgcgc accgcctcga gcagctcttg tccttcggcc
      541 tcgccatgca gaggtcgccc cgagagcagc tcgtgaaaga cgacgccgag cgagtagatg
      601 tccgtgcgcc gcgtgacccg ttcgccgcgc gcttgctccg gcgacatgta agcggtcttg
      661 cctttgagca cgccgggctc ttcgcgaaac aggttcgcag acgcgatgcc gaaatcggcg
      721 attttgacag cgccgtcgta actcagcagg atgttctgcg gtgagacgtc gcggtgaacg
      781 atgtcgagcg gcgcacccgc ttcgtctttg cgctcgtgcg cataatgaag tcctttggcg
      841 acttcggcga tgatgtaagc ggccacatac ggcggaattc gctgtcctct cgcctgcgcc
      901 gccttcatta atcgacgtaa atcgggcccc tcgacatact ccatactaag gagctgtccc
      961 tcttctccgt agtcctggaa gtcgtagacc tggacgatgt tcggatgatt gagacgagtc
     1021 gcgagctgcg cctcctccgc gaacatcgtg cgaaatcgcc gcgagtttcc gtaagcgggc
     1081 aggactcgct tcaaaacgag gagcttgaaa gtgtcctcgg cgccgcgttt cttacctaga
     1141 aacacctcgg ccataccgcc tgcgccgagg cgccgtacga tctcgaaatc cgcgaggcgg
     1201 tggggcgtcg ggcggtcgga cggatcggac atcgtctcgt ctagattacc gcggaccgaa
     1261 cctcgcgtgg taacgtctcg ccccgcttac acccgagctc gctcgggtgg gttggaccag
     1321 aacgatgccg agcgacagaa acgtgcgtac gagcgaagta tctcgcggtc ctcaattgct
     1381 tgggactgcg aaatactcgg tcgctgcacg tggtcgacgg tacaggcggc cgctttgccg
     1441 actaggtcgg cgataatctc ccttttcccc tcatggagca acggatgcga tggtgcgtgg
     1501 ctttggcgct gatctcgacc cttctgagct cgaatgctct cgccgcccct aaggggaaga
     1561 agggcaagaa cacgaagaag gacgacaagt cgggcgccag tatggagacc accggggcgg
     1621 atccggtgag gaccgagacc agcgacccgg gtccgtacgc tccgaaaggg atgaccggag
     1681 cgatgggtga agaggcggag cacaccgaag aggtgaagca ggtcgtgaag gcgaagccgc
     1741 gcgacaaggc gctgctcttc gcggacgtgg tcgtcggttg gggcaactcg ccgaaaccag
     1801 ggccgaaccg cgcgaacgct tccggcggca gagacgatca gctgaacgac gcgatcgctg
     1861 gttcgctcat cctcggcgga cgcttcgatc tttcgcccaa gttctcgctc ggctttcgac
     1921 tgccgatcgg ctcgtcgttg gtcgatcgag gcggcgcgct cgggaagcag agccacgttg
     1981 cggtcggcgc aatcgaagtg ctcggcgagt accgtcacta cctttcgccg ctcacgtcgt
     2041 tgccgattct attcgggctc gggctcccga cgggcaccga cgatccggat ccgagcaccg
     2101 gcgagacttc gaatcgcacg aacaccatcg tcgacagcgc gcgcggctgg cgcgacggcg
     2161 agctcttctg gtcgaagcgc gtcccgctga ttttgggaat cggcattcgc cacgagaacc
     2221 gttcgctcga gcttcgcgcc gcgaccaagg tcgtcgccgg gttccgcatc gctggcgatt
     2281 tggcgaacgc gaactactac ggcgcgccgc gctccgagct gcaaaacaac ttcgtcgcgc
     2341 tccgctcggt gacggacgtc ggcttgtcgt actccttcac ggagaagttc tgggccggcg
     2401 ccgatatctg ggccgccgcg aacatcatcg agccgattca cttcaagtcg accgcggaac
     2461 ccccgttctt gttccaattc gttgcagagc cgatggtcgg tttgcggttc gggaagattc
     2521 gcccgaacct cggcttcatc atcccgctca acggacgcct ggccgacggc gggatgtacg
     2581 gcggccagct tcgcgtcgac gtcgccctgt gacaaaaacg cagatctgcc gagcgccgct
     2641 ggcttttgga gcggccgccc gcgcggtgta aactggtgcc aatgcgcgct cggcgtctgc
     2701 gttcgttgcc tcgtttcggc gttttagcgc tcgctctgag cgcgaacttg ggatgcgcga
     2761 gcgggccgcg tccgcaccca gccaagccca ccgcgacgct cgtcacggga cccgtgcaag
     2821 cggcgcccgt cgacgagcag aacttcgccg agagtacgta tcagctcttg ctcgatggcg
     2881 agccgagtcg tgagcgcgtc ggcaagctca tcggagtcgt acgccaccag ctcgcgcgct
     2941 ccgaggctcg cttcgaaggc ggcagcagcg acgcggggct gcgcgcgctg ctcggcgcat
     3001 tcatgttggt gcgcgctggc gagcaccgcc ccgagctgtt gaacggcggg gaacgtgcgc
     3061 tcggcaacgc cgccaccgag gtcgcgcgct tgggccgcga gggctactcg gtcgcgctgt
     3121 acacgatgct cgcctcggcg ctgccagcgt cgccggcgcg caacgaggtc gaagcgcatc
     3181 tccgcgcgat ccaggatttt tcgcgctcga cccaaggcgg cggacccgtg caggcagccg
     3241 gcatcggcgc gcgcgtcgca gtgcaacgcg ccctgctcga ggcaacggac gagtcgctca
     3301 cggcggcccg tgacgcgatg gtcacctgga ttcgccgtgc gctcgagctc aacgccacgg
     3361 agcagccgat tcgaagcaac ctcgatcgcg acgagtcgat cgaggcttat cgcggcttgc
     3421 acagcggggc catcgcgctc accgctctct acctgcgcca cggggacccg cgcggcgcgc
     3481 tcagcgccat cgatcacgcc gagctgcagc gcctgctctc ccccgagctt cgcgaccact
     3541 tggagcgcat cgcggacgaa c
//
LOCUS       DASTKS010001030         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_832385, whole genome shotgun sequence.
ACCESSION   DASTKS010001030 DASTKS010000000
VERSION     DASTKS010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832385"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..649
                     /locus_tag="VFQ35_21790"
     CDS             353..649
                     /locus_tag="VFQ35_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933358.1"
                     /translation="MVREFEYKGEIFRVTAHGIPGHEEVFVVHIDKEGVEGGETSIDR
                     MTEANDTSAPLESILVMLCDRLTPDIAVQNPSGAFAWREGNVAFNVHDGVQALA"
     gene            complement(726..1898)
                     /locus_tag="VFQ35_21795"
     CDS             complement(726..1898)
                     /locus_tag="VFQ35_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933359.1"
                     /translation="MAIGGWAAPVLPTQARSRWLLGLAFFGAASVFAAFMAVRHVPWF
                     GPLVADSLRRVVGSENVTTLEETVATVEDRVHSAVSDGRARSLSEATPVELAQETKTV
                     VPSPFRSQLRDVGALYAATKSADDGKWQPVRVRGSDTSPLYRTIVHPDAERSYAELFV
                     FALDLSQVKVQAVAGSIEPKNPDASSDLARPGIVPSEARPKLIAAFNGGFKAEHGQFG
                     MMADGQELLAPKPRSCTFAERADGSLTIATWSTLAAKDNAFSWWRQTPGCMIEKGKLH
                     PGLLTEDSTNWGATLEGKTVIRRSAAGLSEDGRTLFVGISNSTTARALALGMQHVGAR
                     DVAQLDVNFSFPRFLLYRDAEAGELQAVGAVRGLLYEKDEYLGKASTRDFFYVTTR"
     gene            complement(2209..3330)
                     /locus_tag="VFQ35_21800"
     CDS             complement(2209..3330)
                     /locus_tag="VFQ35_21800"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET9933360.1"
                     /translation="MTRISKPATLALGATLALGATSCSEPDETRALLKALEKTAVSTA
                     QAAEKKSNVDINPRLLRRFQPLRARVESDKNPLLESKIQLGRMLYFEKRLSKNQELSC
                     NSCHDLATYGVDHVSFSTGDRGQKGGRNAPTVYNAAGFFAQFWDGRAQSVEEQALGPI
                     LNPVEMALPSAAHGVKVLKSMPEYVTRFKQAFPDETDPVTFENAGKAIAAFERTLMTP
                     SRWDDYLRGNEAALTDPELEGLKIFTNIGCMVCHTGEFVGGAMYQKVGVVEPWPNQKD
                     QGRFEVTKQEVDRMMFKVPTLRNIAQTAPYFHDGSAPTLDVAVRMMGKHQLGLSLSDR
                     EVTSIVTWLGSLTGKLPSDSIKAPELPPSSPTTPAANTQ"
     gene            complement(3332..>4395)
                     /locus_tag="VFQ35_21805"
     CDS             complement(3332..>4395)
                     /locus_tag="VFQ35_21805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9933361.1"
                     /translation="KMLLIISDVTALVERERSEQEERDVLSLMSRLLHDRAGFLEFFN
                     ETQGLITKIHSNKSDIVHLKRDLHTLKGNTAIFGLSRLSGTCHELENELETLTADELD
                     SSALVEQWQRLSAKVKVLLGEGEQTSIAVDAREYDRVLEAVRKGAPSSSIRPLIEAWK
                     LESLSTRLGRVSQQLIRTVERVGKGHAEVDLNVPRIYLAREELAEFWSVFSHVVRNAA
                     VHGLEPLEVRSELGKPSVGRYLLSAGVQKERLFIELRDFGPGVDWVKVRERAEQKGLP
                     SNTPQDLERALFSDGISTEREVSDTAGRGVGLSAVREVCLRQGGEITVSSKRGEGASF
                     RFSWPVSRLRSLTVLEGAA"
BASE COUNT          757 a         1414 c         1409 g          815 t
ORIGIN      
        1 aagacgtggt tgcgtcattt cgtgaacgag acgccgagcg ctgcgtgcgg acgccgccgg
       61 gaggagcgtc gggatgacgt aacgctcgct cgccctgcat tccttagaac gagcgtcttg
      121 cggcgcgctg ccaatcgtcg aaagcgcgcg cgggctgaac ggctcgcgcg cggtcgtgta
      181 tttgccgact cgagactctg gtgtgagcgc actagcgcga gggtatgacg cgaattgttg
      241 caagtcagtt ctcgtctcgt tgccactcgc gtcgcgagcc tcgattgagg tagttagtgc
      301 acgctcggcg tctcgctcga gcgtcactca ttcaacccag ttgagaggca ccatggttcg
      361 tgaattcgaa tacaagggcg aaatctttcg cgtcaccgcg cacggcatcc cgggccacga
      421 agaagtcttc gtcgttcaca tcgacaagga gggcgtcgaa ggcggcgaaa cgtcgatcga
      481 tcgcatgacc gaggcgaacg atacgagcgc gccgctcgag agcatcctcg tcatgctctg
      541 cgatcgtctg acgcccgaca tcgccgtgca aaatccgagc ggtgctttcg cttggcggga
      601 aggcaacgtc gccttcaacg ttcacgacgg cgtccaagct ctcgcgtgag cgagctttcg
      661 tgggaagcgg cgctaccgtt accaggagcg ccgcaatccc gagctcgctc cgatgacgcg
      721 agccctcagc gcgtcgtcac gtagaagaag tcgcgcgtcg acgctttgcc gaggtactcg
      781 tccttttcgt agagcaatcc gcgcactgcg ccgacggctt ggagctcgcc cgcctccgcg
      841 tcgcgataga ggaggaagcg agggaacgag aagttcacgt cgagctgcgc cacgtcacgc
      901 gctcccacgt gttgcatgcc gagcgcgagc gcgcgcgccg tagtcgagtt gctgatgccg
      961 acgaacagtg tgcggccgtc ctcggagagg ccagccgcag agcggcgaat gacggtcttc
     1021 ccttcgagcg tggcgcccca gttcgtcgaa tcctcggtca gcaaacccgg gtgcagcttg
     1081 cctttctcga tcatgcagcc gggggtctga cgccaccatg agaaggcgtt gtccttcgcc
     1141 gcgagcgtgg accaggtcgc gatggtgagc gagccgtcgg cgcgctccgc gaaggtgcac
     1201 gacctcggct tcggcgcgag gagctcctga ccgtcggcca tcatgccgaa ctggccgtgc
     1261 tcggccttga acccaccgtt gaaggcggcg atgagcttgg gacgggcctc gctcggtacg
     1321 atacccgggc gagcgagatc agatgaagcg tcggggttct tcggctcgat gctgcccgcg
     1381 acggcttgca ctttcacttg cgacaggtcg agcgcgaaga cgaagagctc ggcgtacgag
     1441 cgttcggcgt ccgggtggac aatcgtccga tacagcgggg acgtatcgct gccgcgcacc
     1501 cgtacgggtt gccacttgcc gtcatccgcg ctcttggtcg cggcgtagag agcgccgacg
     1561 tcacgcagtt gactacgaaa tggcgacgga acgacggtct tcgtctcctg cgcgagttcg
     1621 accggagtcg cttcgctcag gctgcgggcg cgcccatcgc tcaccgcgga atgaacccgg
     1681 tcttccaccg tagcgacggt ttcttcgagc gtggtcacgt tctcgctgcc gaccacgcgg
     1741 cgcaggctat cggcgacgag cggcccgaac cagggcacgt ggcgcaccgc catgaaagcc
     1801 gcgaatacgc tggcagcgcc gaagaatgcg agcccgagca gccagcgcga gcgtgcttgt
     1861 gtcggcagaa ccggcgccgc ccaaccaccg atagccacca gcgcttcgtg tcccggcacg
     1921 agacgcagtg ggaaaccgtg agggtgtgac gaaggaacgc gcgcgaccat gactgttcaa
     1981 gctccgcgca ccctttctgc accccacgtg ccaggcagaa accgccgccg agcggcgtat
     2041 cttcctacgc agaccccgga ccacgtatct cgaaaatcct aaccggttca atgggttagc
     2101 gatgcgttca gtgcagctgt cgcatcgagg tgcgagcgtc acgcatcgag agctgttgcg
     2161 taactcaccc agccccgagt gtaggcggac acggcccgcc cgctcgtatc actgcgtgtt
     2221 cgcggccggt gtcgtcgggc tgctgggcgg gagctcgggc gccttgatcg agtcgctcgg
     2281 cagctttccg gtgagggagc cgagccacgt cacgatcgaa gtcacttcgc gatcgctcag
     2341 gctcaacccg agctgatgct tgcccatcat gcgcaccgcc acgtcgagcg tcggcgccga
     2401 gccatcgtgg aagtatggcg cggtctgggc gatgtttcgc aaggtcggta ccttgaacat
     2461 catgcggtcg acctcttgct tcgtcacttc gaagcggccc tggtctttct ggttcggcca
     2521 cggctcgacg acgcccacct tttgatacat cgcgccgccg acgaactcgc cggtgtggca
     2581 gaccatgcag ccgatgttcg tgaagatctt gagcccctcg agctccgggt ccgtgagagc
     2641 ggcttcgttg ccgcgcaaat agtcgtccca gcgcgacggc gtcatcaggg tccgctcgaa
     2701 cgccgcgatc gctttgccgg cgttctcgaa agtcacgggg tccgtttcgt ccggaaacgc
     2761 ctgtttgaag cgagtcacgt actccggcat cgacttcaac actttcacgc cgtgggccgc
     2821 gctcggcagc gccatttcga ctgggttcaa aatcggtccg agcgcctgct cttcgacgct
     2881 ctgcgcgcgc ccgtcccaaa actgcgcgaa gaagcccgcc gcgttgtaca cggtgggtgc
     2941 gttgcgaccg cccttttgtc ctcggtcccc cgtagaaaac gagacgtgat cgacgccgta
     3001 cgtcgcgaga tcgtggcagc tgttgcacga gagctcttgg tttttggaga gccgcttctc
     3061 gaagtacagc attcgcccga gctgaatctt ggactcgagc aggggattct tgtcgctctc
     3121 cacgcgcgcg cggagcggtt ggaagcggcg caaaagacgg gggttgatgt cgacgttcga
     3181 cttcttctcg gcggcttgcg ccgtagaaac ggccgtcttc tcgagcgcct tgaggagagc
     3241 gcgcgtctcg tcgggctcac tgcacgaggt cgcgccgagc gcgagcgtgg cgccgagcgc
     3301 aagtgtggcc ggtttcgata tgcgggtcat ctcaagcagc tccttccaac acggtcaacg
     3361 aacgtagacg cgataccggc caggagaagc ggaacgacgc gccttcgccg cgcttgctcg
     3421 aaacggtgat ttccccgcct tggcgcagac aaacttcgcg tacggcgctc agcccgacgc
     3481 cgcggccggc agtgtcgctg acctcgcgct cggtcgagat gccatcgctg aacagcgctc
     3541 gctcgagatc ctgaggagtg ttggacggta agcccttttg ctcggcgcgt tcgcgcacct
     3601 tcacccaatc cacgccggga ccgaagtcgc ggagctcgat gaagagccgc tctttctgca
     3661 ctccggcgct gagcagatat ctgccgacgc tcggcttacc gagctcgctg cgcacctcga
     3721 gcggttcgag cccgtgcacg gccgcgttgc gcacgacatg ggagaacacg ctccagaact
     3781 cggcgagctc ttcgcgcgct aggtaaatgc gcggcacgtt caggtcgact tccgcgtgcc
     3841 ccttgccgac gcgctccacg gtgcggatca gctgctgact cacgcggccg aggcgagtcg
     3901 agagcgactc gagtttccac gcctcgatca acggacgaat cgagctgctc ggcgcgccct
     3961 tgcgtaccgc ctcgagcacg cggtcgtact cgcgcgcgtc gactgcgatc gacgtctgct
     4021 ccccttctcc gagcagcacc ttcaccttgg cggagaggcg ctgccattgc tcgacgagcg
     4081 cggaagagtc gagctcgtcg gcggtgagcg tctcgagctc attctcgagc tcgtggcagg
     4141 tgccggacag gcgcgacagc ccgaaaatcg cggtgttgcc cttcagcgtg tgcagatcgc
     4201 gcttcagatg cacgatgtcc gacttgttcg aatgaatctt ggtgatgagg ccttgcgtct
     4261 cgttgaagaa ctccaagaag ccggcgcggt cgtggagcaa gcgcgacatc aggctcaaca
     4321 cgtcgcgctc ttcttgctca ctgcgctcgc gctcgaccag cgcggtcacg tccgagatga
     4381 tgagcagcat cttct
//
LOCUS       DASTKS010001031         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_832430, whole genome shotgun sequence.
ACCESSION   DASTKS010001031 DASTKS010000000
VERSION     DASTKS010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832430"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VFQ35_21810"
     CDS             <1..1648
                     /locus_tag="VFQ35_21810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9933362.1"
                     /translation="IVALVGNIGRTTSDELGERVHAMKTDVVDAIIYLGDVDREMQLY
                     RGDVWKYLALPADTDKAATLREVESRPASIESMFRKYSQTQAFPGEGEARQDFLTAWS
                     QIVAIRTRAVSLAQSNPAEAARVLLRDGTPPDRAARAALKKIMEINVSQASLQATTAV
                     ESSRSAARQILLYTGAAAALAVMLGWLISRAITGAVDQVTHAAERIAAGDLDVEIVLE
                     QKDEIGRLAETQRKMTAQLRSTMMQIQTVAEHVAAGSEEMTASAEQLSHGATQQSSSA
                     EEASASIEEMSGSIAQNADNAAQTERIAVQTAQDAERSGVAVGKSVSAMHEIAAKISI
                     VEEIARQTNLLALNAAIEAARAGEHGKGFAVVAAEVRRLAERSQVAAGEITNISSASV
                     RAAEEAGEMLKATVPAIRKTSTLVQEISASTREQSTGSQQISVAIQQLDQVIQQNAAA
                     ASQLSSTSSEFSVQAQRLQELVSFFRISGTHNVAPPPRTESPRRSAPRQRPRKSSQFP
                     KAQPAALARAAGHDVVPSNTSRGAVVRLGPDSDDADFEAS"
     gene            complement(1673..>2356)
                     /locus_tag="VFQ35_21815"
     CDS             complement(1673..>2356)
                     /locus_tag="VFQ35_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9933363.1"
                     /translation="GAVSNKPGLVEMANRGTLFLDEIGDLDPAVQPKLLKVLEDRSFR
                     RLGDVQSRRADVRLVAATHRDLATMAKSGTFRQDLLYRINTLTIELPPLRNRSQDIPE
                     LSRAVLRELAQAQGRPVPTLSDDALEMLISYSWPGNIRELRNVLERAVLFCKGSTIER
                     TTLAFDRALLPETTPEPQTLDDAERRHIASVLRLAGGRVDDAARVLALSRSSLYAKLK
                     KYGIKTAER"
BASE COUNT          457 a          778 c          758 g          363 t
ORIGIN      
        1 catcgtcgcg ctcgttggca acatcggtcg taccacgagt gacgaactcg gcgagcgcgt
       61 acacgcaatg aagacggacg tcgtcgacgc gatcatctat ctcggtgacg tcgaccgaga
      121 gatgcagctc taccgcgggg acgtatggaa gtacctggct ttgcccgccg acacggacaa
      181 ggcggcgacg ctccgtgagg tcgagtcgcg ccctgcgtcg atcgagtcga tgttccgcaa
      241 gtactcgcag acccaagcgt tcccaggcga aggcgaggcc cgccaggact ttttgacggc
      301 ctggtcgcaa atcgtcgcca tccgcacgcg cgcggtttcg ctggcgcaga gcaaccccgc
      361 ggaagccgcg cgagtgctgc ttcgcgacgg gacgccaccg gatcgcgccg cgcgggccgc
      421 cctgaagaag atcatggaga tcaacgtcag ccaggcgagc cttcaagcca ccaccgccgt
      481 cgagagcagt cggtctgccg cacgacagat cctgctctat accggcgccg ccgccgcgct
      541 cgcggtgatg ctcggctggc tgatttctcg ggcgatcacc ggcgcggtcg accaggtgac
      601 tcacgccgcc gagcgaatcg ccgcgggaga cctcgacgtc gagatcgttc tcgagcaaaa
      661 ggacgagatc ggtcggctcg cggagaccca gcgcaagatg acggcgcagc tccggtccac
      721 gatgatgcaa attcaaaccg tcgcggagca cgtcgcggct ggcagcgaag aaatgacggc
      781 ttccgcggag caactgtcgc acggcgcgac gcagcagtcc tcttccgccg aagaggcatc
      841 cgcctcgatc gaagagatgt ccggcagcat cgcccaaaac gccgacaacg ccgcgcagac
      901 ggagcgaatc gcggtgcaga ccgcgcaaga cgccgaacgc tccggcgttg cggtcggaaa
      961 gagcgtgagc gcgatgcacg agatcgccgc taagatctcg atcgtagaag agatcgcccg
     1021 gcagacgaac ctgctcgcgc tgaacgccgc gatagaagcg gcgcgcgccg gcgagcacgg
     1081 caaagggttc gcggtcgtgg ccgcggaagt gcgacgcctg gccgagcgca gccaagtagc
     1141 cgccggagag atcacgaaca tctcgtccgc gagcgttcgc gccgcggaag aagccggcga
     1201 aatgctcaag gcgaccgtcc cggcgatccg caaaacatcc acgctcgtcc aggaaatctc
     1261 ggcctcgacg cgcgagcaga gcacaggctc ccagcagatc agcgtcgcga ttcagcagct
     1321 cgaccaagtc attcaacaga atgccgcggc cgcctcgcaa ctatcttcca cgtcttcgga
     1381 gttttcggtg caagcgcagc gccttcagga gctcgtctcg tttttccgca tcagcggaac
     1441 ccataacgtc gcgccgccgc cgcgtaccga gtccccccgt cgcagcgctc cccgtcagcg
     1501 accgcgcaag agctcgcagt ttcccaaggc tcagcccgca gccctggcgc gcgcagccgg
     1561 acacgacgtc gttccctcga acacttcgcg tggagccgtc gttcggctcg gtcccgacag
     1621 tgacgacgcg gacttcgaag cgagctgaac gagacgacgg tagctcgcgc gctcaccgct
     1681 cggcagtctt gattccgtac tttttcagct tcgcgtagag agagctgcgg gagagcgcca
     1741 aaacgcgggc cgcgtcgtca actcgccccc ccgccaagcg caggacggaa gcgatgtgac
     1801 gccgctcggc atcgtcgagc gtctgaggtt ccggcgtcgt ctccggcaaa agcgcgcgat
     1861 cgaacgcgag ggtcgtgcgc tcgatagtgg agcccttgca gaataaaacg gcgcgctcga
     1921 gcacgttgcg aagctcgcgg atgttgcccg gccaagaata gctgatcagc atttcgagcg
     1981 cgtcgtcgct caacgtcggt acgggccggc cctgcgcttg ggcaagctca cgcagcacgg
     2041 ctcgcgagag ctccgggatg tcctgactgc gattgcggag cgggggtagc tcgattgtca
     2101 gggtgttgat tcgatacagc agatcttgcc gaaaagttcc gcttttggcc atcgtagcga
     2161 gatcgcggtg cgtcgccgca acgaggcgaa cgtccgcgcg tcgcgattgg acatcgccga
     2221 ggcggcgaaa agagcggtct tccaggacct tcaggagctt gggctgcacg gcgggatcca
     2281 gatcgccgat ctcgtctaga aatagcgtgc cccggttcgc catctccact agccccggct
     2341 tgttcgagac ggcgcc
//
LOCUS       DASTKS010001032         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_832742, whole genome shotgun sequence.
ACCESSION   DASTKS010001032 DASTKS010000000
VERSION     DASTKS010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832742"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFQ35_21820"
     CDS             <1..536
                     /locus_tag="VFQ35_21820"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HET9933364.1"
                     /translation="QDTRLRILTRDREQALSAVLLGEADLAVTVADEVPPAVLARPLL
                     RVSASVVLPAAHPLARKRAVSLASLSGERLIVPPLGRPLRAALAHAFSEARVSFTPSI
                     EVTGWELMLCFAELGLGVAIVNDFCKAPKGTVKRPLRGLPSVQYQLLRLRERRPTESA
                     RALEHAVESEVRKLDRS"
     gene            complement(593..1309)
                     /locus_tag="VFQ35_21825"
     CDS             complement(593..1309)
                     /locus_tag="VFQ35_21825"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET9933365.1"
                     /translation="MQKKTLAGLVTALATAVALASLAAPTIHPERPTTLRALYGLKAP
                     ERLSARRTALVLVDFQDEFFHGRLVLRDGAAAASAAKELLSWARRSNVLVVHVRNVAA
                     RADSPVFRAGSSAVEIVPELRPLPDEMVIQKSSGGAFTHTELDQRLRARDIDTLVVCG
                     LMTHLAVATSAADATALGYRVVVAADAVATRELPGVLTPASVDATTLQRASLAALADR
                     MADVISVRAIVTLPLERSGG"
     gene            complement(1329..2330)
                     /locus_tag="VFQ35_21830"
     CDS             complement(1329..2330)
                     /locus_tag="VFQ35_21830"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9933366.1"
                     /translation="MKAYVFEKRLALDELKPVERPIPTPKSHEILVAMRAVSLNFRDL
                     AIARGDYGNFPCPLVPLSDGAGEVVACGASVTRFSKGDLVCAAYVPDWIDGPVSERVA
                     RRRLGGPSDGVLAEYICVDESAAVRAPAHFDAREAATLPVAAVTAYEALFRGARLAPG
                     DVVAVQGSGGLSLSVLQLARAVGARVLALTRDDSRRERLERLGAEVFVVSDSRWDVPL
                     RAATNGEGVDVFVDVVGGPSLQAAVNSTRFGKTLLLLGFVAGQQSEVDLVGAIRRAIT
                     LRAVSGGSRESFEALTRGLQNWQLRPVIDRVFALRDLHAAFEHLARGRPFGKVVVEF"
     gene            2406..>2968
                     /locus_tag="VFQ35_21835"
     CDS             2406..>2968
                     /locus_tag="VFQ35_21835"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9933367.1"
                     /translation="MNWDDLRFFLALYRAGSYKAAARAQRVDATTVGRRVAALELALA
                     VKLFTRGPERALPTAAGRALLARAERIEAEVLASRREIESAEERVEGALRVTAGDGLV
                     NYVLVPALPSLTRRYPELSLELRGETRALDLGRSEADVALRLFRPRERSLVVRRLGAC
                     TFNLFASRLYLERHGAPRTVDALGQHV"
BASE COUNT          502 a         1035 c          961 g          470 t
ORIGIN      
        1 cccaggacac acgactcagg atcctgactc gggatcgtga acaagccctt tcggcggtgt
       61 tgctcggcga agccgacctc gccgtgacgg tggccgacga agtacctccg gcggtgctcg
      121 ctcgtccgtt gctgcgcgtg agcgcttcgg tcgtgctccc cgcggcgcat cctctcgctc
      181 gaaagcgcgc cgtctcgctc gcttctcttt cgggggagcg actgatcgtg ccgccactcg
      241 gtcggccact gcgtgcagcg ctcgctcacg ccttttcaga ggcgcgggtg tcgttcactc
      301 ccagcatcga ggtgacgggc tgggagctca tgctctgctt cgccgagctc ggcctggggg
      361 tcgcgatcgt aaacgacttc tgcaaggctc cgaagggaac ggtaaagcgg ccactgcgag
      421 gcttgccttc ggtgcaatac cagctgcttc gccttcgcga acgccggcca accgagtcgg
      481 cgcgcgcgct cgagcatgcc gtcgaaagcg aagtgcggaa actcgatcgc tcatgagtcg
      541 gtgcagcgcg ggtgagattg accttgctgg cgcgtatctc atacgcgtgg cactagcctc
      601 cgctccgctc gagtgggagg gtcacgatgg ctcgaaccga gatcacgtcc gccattcgat
      661 cggcgagcgc ggccaggctc gcccgctgca gtgtcgtcgc gtctacgctc gcgggcgtga
      721 gcacgcccgg cagctctcga gtcgcgaccg cgtccgccgc gaccacgact cggtaaccga
      781 gcgcggtggc gtcggcggcg ctcgtcgcga ccgcgaggtg ggtcatcaaa ccgcaaacga
      841 ccagcgtgtc gatgtcccgc gcgcgcaggc gctggtctag ctcagtgtgg gtaaaggcgc
      901 cgccgctcga cttctgaatg accatctcgt ccggtagtgg acgcagctcc ggaacgatct
      961 cgacagcgct actccccgcg cgaaaaacgg ggctgtccgc gcgcgcggcc acgttgcgca
     1021 cgtggacgac gagcacgttc gagcgccgcg cccaggacaa gagctccttg gccgcggaag
     1081 cggccgcagc gccatctcga aggacgagac ggccgtgaaa gaactcgtct tgaaagtcca
     1141 ccagcacgag cgcggttcga cgagcgctca gccgctccgg agccttgagg ccgtacaagg
     1201 ctcgcaacgt cgtcgggcgc tcgggatgaa tcgtcggcgc ggcgagactc gcgagcgcca
     1261 ccgccgtagc gagcgcagtc accaaaccag ccagagtttt cttctgcatg atgctctcct
     1321 tctcctcctc aaaactccac gacgactttt ccgaacgggc gcccgcgcgc caagtgctcg
     1381 aaggccgcgt gcaggtcgcg caacgcgaac acgcgatcga tgacgggccg gagctgccag
     1441 ttttgcagcc cccgcgtgag cgcttcgaag ctttcgcggc taccgccact gactgctcgc
     1501 agcgtgatcg ctcgacgaat cgccccgacc aagtcgacct cgctctgctg tcccgccacg
     1561 aagccgagta gcaagagcgt ctttccgaag cgcgtcgagt tcaccgccgc ttgcaggctc
     1621 ggcccgccga cgacatccac gaaaacgtcg acgccctcgc cgttcgtcgc tgctcgcagc
     1681 ggtacgtccc agcgcgagtc gctcacgacg aacacctccg cgccgagccg ctcgagccgt
     1741 tcgcggcgcg agtcatcgcg tgtcagcgcc agaactcgag ctcctaccgc gcgcgcgagc
     1801 tgcagcacgg acagcgagag accgccgctg ccctgaacgg cgacgacgtc gccgggggcc
     1861 aaacgcgcgc ctcgaaacag cgcctcatac gccgtcaccg ccgcgaccgg aagcgtagcg
     1921 gcttcacgcg cgtcgaagtg cgcgggcgcg cgaacggcgg ccgactcgtc gacgcaaatg
     1981 tactccgcga gcacgccgtc gctcggacca cccaagcgcc gtcgagcgac gcgctcgctc
     2041 acgggaccgt cgatccaatc cggcacgtac gccgcacaga cgaggtctcc cttcgaaaat
     2101 cgagtgacgc tggcgccgca cgctacgacc tcgcccgcgc cgtccgaaag cggaaccagg
     2161 gggcacggga agttgccgta gtcgccgcga gcgatcgcga gatcgcggaa gttgagcgaa
     2221 accgcgcgca tcgcgaccag gatttcgtgc gactttggcg tggggatcgg gcgctctact
     2281 ggcttgagct cgtcgagggc gagccgcttc tcgaaaacgt aggctttcat gggtccgaat
     2341 ctggtccaga acccgcacct gcaaaagtcc gtgatattgc agttcgtacc tgcaaatatg
     2401 cagccatgaa ctgggacgat ctccgcttct ttctcgcgct gtaccgagcc ggcagctaca
     2461 aggccgccgc gcgcgcacaa cgcgtcgatg cgacgacggt ggggcgtcgc gtcgcagcgc
     2521 tcgagctcgc cctcgcggtg aagctgttca cgcgcggacc cgagcgagcg ctgcccacgg
     2581 ctgcgggcag agcgctcctc gcacgagccg aacgaatcga ggcggaggtg ctggcgagcc
     2641 gccgcgaaat cgagtccgcg gaagagcgcg tcgaaggagc gttacgcgta accgccggcg
     2701 acgggctcgt caattacgtg ctcgtccccg ctcttccctc gctgactcga cgttacccgg
     2761 agctcagcct cgagctgcga ggtgaaacgc gggccctcga cctgggacgc agcgaggccg
     2821 acgtcgcgct ccggctgttc cgaccgcggg agcgctcgct cgtcgtgcgt cgactgggag
     2881 catgcacgtt caacttgttc gcgagtcggc tctacctcga gcgccacggc gccccgcgca
     2941 ccgtagacgc gctcggtcag cacgtctt
//
LOCUS       DASTKS010001033         8165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_610016, whole genome shotgun sequence.
ACCESSION   DASTKS010001033 DASTKS010000000
VERSION     DASTKS010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610016"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..2018)
                     /locus_tag="VFQ35_21840"
     CDS             complement(327..2018)
                     /locus_tag="VFQ35_21840"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933368.1"
                     /translation="MARTLIGPVLSFSVACVVACGNGKTDGGSPPFTTGGTAGVSTNG
                     SGGTAVNGAGGSNTTSGSASGSNGSGGASSSSGSGPSTSSGGVSPSSGGSSFGGSSSM
                     PNPQLPNGYWAVGSWKGRAWGKTTGTGSTMMPAAFDSVPFGMPYCASGSVGPMSDYSG
                     TATVGVNLNQEADPSAPANPVTPTKDGITVNVTNRGGSALRVELRGPNAETDTAQRWC
                     ASIMGSGGFIPWSSFNTACWDGSGAAYNREPIVSAAILVPGSNSNAVSYDVCLNSLNE
                     GMPGATSGSGGASGGGGASSSSGGSNGNRGGASNQGGVTGSGTAGTAGGDPKPIMNSG
                     TLKDRYDWQPVEHEGRNYVVQNNVWGSGASQTVTYNETSFKVTAQSGTNGTNGAPVSY
                     PSTFIGSNYERTTMGSNLPKLVSSIASVQTSWTNNAGGGIGGTYNASYDVWFSTKAEG
                     DPEAPSGGYLMVWFYDPANAQPIGSVVASNVSVSGVSGSWNIWLGNNGPVPCISYVRT
                     QSIQSMTFDLNAFIQHAVGRGTIKSNWYLSNVFAGFEIWNGGVGLESSGFYAIVN"
     gene            complement(2361..3539)
                     /locus_tag="VFQ35_21845"
     CDS             complement(2361..3539)
                     /locus_tag="VFQ35_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9933369.1"
                     /translation="MPNARVAEFLLDPALTFLNHGSFGACPRVVLDAQLAYRERLERD
                     PVRFLAGDLEGLLDVARQSAAAFVDAAPEDFVFVRNATSAVNTVLASFPFRAGDELLV
                     TDHGYAACRNAAEYWAARASARVVEACLPWPLSDADAALDAIRRVLTPRTRFALVDHV
                     TSPSALVLPIERIVAELRDRGVTVLVDGAHAPGMLPLSVRAVGADYYTGNFHKWCCAP
                     KGAAFLATTPARRGTLRPLVVSHGATADRPERPRAWLEFDWVGTEDPTAYLTVPHALD
                     FIRTALPGGLAALYQQNRTLVLEARALLGERLGAVPSAPSDFVGSMATVTLPAAGLPA
                     PAALSRRLLEEHRIEVPVFTVNGSRDCAFRVSAHLYNALEDYQHFASALELIVRSPAN
                     "
     gene            complement(3589..4989)
                     /locus_tag="VFQ35_21850"
     CDS             complement(3589..4989)
                     /locus_tag="VFQ35_21850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9933370.1"
                     /translation="MKVEFRISLGITGVVAVALATALTLIQVLLNRFQEHQLDQALVE
                     LAHQEARAAPLHNFALTTHPGPAANDVGPLDKYGIIYGAGDKVIAATEPFDAAPPSPD
                     ELFKERVGVPFDMHYRGRSLRAVLVPLATRERATLLLATSRSDLDGDDEFLELAMLLA
                     FFASIGCVLISALWLTRRFTREHRRIATTLHSVARGDLYARAPMGHADPDLEQWANDL
                     NGVAERLGVLIRSQQRFIANASHELRSPLAALYGELQQSLRRERSAEDYKASITQALY
                     ATRRLKHLADDLLTLARAEHTRQEFEPVPLARVFDEVARELARQISEKSIRLEAELAD
                     LSVRGRPGDVQRLLRNLVDNAIRHSPAGGAVRCSARAEGERTVLLVSDEGPGVPEVDR
                     ERIFEPFFRSPETRSTAQDSAGLGLSIAREIARAHGGDLRVEDSPRGAVFSVELCSSS
                     ADATRDSPKGAAASAG"
     gene            5172..5834
                     /locus_tag="VFQ35_21855"
     CDS             5172..5834
                     /locus_tag="VFQ35_21855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9933371.1"
                     /translation="MRILLVEDDLQLGELLRRILSEEGHALEFTSSLADARRALEQQR
                     VDVIVLDRMLPDGDGLEFCGALRSQGDETPILMLTARTEVQDRVAGLRSGADDYLGKP
                     FEVEELLARAEALVRRSASSWLLELGPLRIDRRARTVEAGGVRVDLTAREYGLLERIA
                     VGRGEPVSRQTLLEDVWNMRFDPGTSMLNVHVSRLRDKLRSFAWLVETVPNFGYRLRS
                     TR"
     gene            complement(5882..>8165)
                     /locus_tag="VFQ35_21860"
     CDS             complement(5882..>8165)
                     /locus_tag="VFQ35_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9933372.1"
                     /translation="QGDADVVSGTVMMLMGANSRDVVHAVGERVQEIAKTLPPGVSIE
                     AYYDRAEFVGSTIRTVMTNLAEGVAIVLLVLALFLGSIRGALAVVIGVPASMSVALIG
                     MRLFGVTGDLMSLGAIDFGFLVDGPIVLLESVIAATAAKQLASDARAQAYANAAASVA
                     RPVAFAVAIIMLVYVPLLTLEGAEGKMFRPMAITMACALFGALIYAVLFFPALLVTLV
                     PPAKDHGPHWIERICQTYARALPGVLRARAYWIIGATVALGAMGYLFSRAGAEFVPRI
                     FEGDLVVAIRRAPSISLPMARELDVKTHAVLRKLPEVKTTVALTGRAEVALDTVGNDS
                     TDIVVKLHEKKKWRDGLDFDGLSAEIKRAVETQVPGTFVSVSQPIEDKTNEILSGSRA
                     DVQIQVFGRDLEEMTRITNDIGRLVMGIQGTGDVRIERAFGQPAITVNADREKMSRFG
                     VRVEDAFSAIQAAREGIEVGNVYDGLRRFAIRVLYPPATPTATSLGELFVSSHTGNLI
                     PLSSITHISEGDGVAAVRHKNRERALRVDVNLRGRDLVSWVAEAQGVIRSQVSTPSGY
                     RIEWGGQFANFERASARLKIVIPVVVAIIFGMLFTMFQNMRTTLAVMALVPLSLTGGM
                     VGLLLRDMPFSLPAAVGFIALGGIGVLNGVVMATEVRRRIDQGASLRAAVVEGCGSVC
                     RAVLTTTAVAALGFLPMMLATGAGAEVQQPLATVVVAGMAFGTALTLFVLPGILSLLL
                     KSPAESRAAVSEPTQASIAA"
BASE COUNT         1388 a         2645 c         2715 g         1417 t
ORIGIN      
        1 tgagcacctt ccggcgagcg cgaacgagct gtagcgccgc tgcgagcccc gagtagctgc
       61 cgccgacgac gactacttca tacatgtttt ccgccttgct ccgcgaattg gctcgatagc
      121 gttggggacc cttctctgag agcccctcac aggatcgagc caccgtcaag gcgacgtcgg
      181 acagggcgcc tctttccggc gaactcatgc cgaccgcgaa ttggcccagc gcgagctcgg
      241 gtgtcaggca gccgttcagg ctcctcggcc cgcgctcacg aggcgatgcg agcgcgggcg
      301 ggacgccatc ttaccgaagc tttggctcag ttcacgatgg catagaagcc gctgctctcg
      361 agcccgacgc caccgttcca aatctcgaag ccggcgaaga cgttgctcaa gtaccagttg
      421 ctcttgatcg tgccgcgacc gaccgcgtgc tggatgaagg cgttcaggtc gaacgtcatc
      481 gattgaatcg attgggttcg aacgtaggag atgcagggta cggggccgtt gttgccgagc
      541 cagatgttcc aagagccgct cacgccggag acgctgacgt tggaagcgac gaccgagccg
      601 atcggttggg cgttggctgg gtcgtagaac cagaccatga gatagccgcc ggacggggcc
      661 tccggatcac cttccgcttt ggtgctgaac caaacgtcgt acgatgcgtt gtacgttccc
      721 ccgatgccgc cgccggcgtt gttggtccag ctcgtttgaa cgctggcaat cgaactcacc
      781 agctttggca ggttgctgcc catcgtcgtg cgctcgtagt tcgaaccgat gaacgtcgaa
      841 gggtaagaca cgggcgcgcc gttcgtgccg ttcgtaccgg attgcgcggt caccttgaac
      901 gaggtctcgt tgtacgtgac cgtttggctc gcccccgagc cccacacgtt attttggacg
      961 acgtagttgc gcccctcgtg ttccacgggc tgccagtcgt agcggtcttt gagagtgccg
     1021 ctattcatga tcggcttcgg gtcgccgccg gcggtgcccg ctgttccgct gcccgtgaca
     1081 ccgccttggt tggacgcgcc gccgcggttc ccgttcgagc cgccgctcga gctcgaggcg
     1141 ccgccgccac cggacgcacc gcccgagccc gaagtggcgc cgggcatgcc ttcgttgagg
     1201 ctgttcaggc agacgtcgta gctaacggcg ttcgagttgc tgccgggcac caggattgcg
     1261 gcggagacga tgggttcacg gttgtacgcg gcgccggagc cgtcccagca cgccgtgttg
     1321 aagctgctcc aaggaatgaa accgccgcta cccatgatgg acgcgcacca gcgttgagcg
     1381 gtgtcggtct cggcgttcgg cccgcggagc tcgactcgga gggccgagcc gccgcgattc
     1441 gtgacgttca cggtgatgcc gtctttcgtt ggcgttaccg ggttcgccgg tgcgctcggg
     1501 tcggcctcct ggttcaggtt cacgccgacg gtcgccgtgc cgctgtagtc ggacattggg
     1561 ccaacgctgc ccgaagcgca atacggcatg ccgaagggga cgctgtcgaa ggcggcgggc
     1621 atcattgtgg aaccggttcc agtagtcttg ccccaagcgc ggcctttcca gctcccgacg
     1681 gcccagtagc cgttcgggag ctgcgggttc ggcatgctcg agctgccgcc gaagctgctg
     1741 ccaccgctcg aaggcgagac gccgccgctc gatgtgctcg gacccgagcc cgaactggaa
     1801 ctcgctccgc cgctcccgtt cgaaccgctg gcggagccgc tcgtcgtgtt cgatccgcct
     1861 gcgccattca ctgcggtgcc accgcttcca ttggtggata cgcctgcagt cccgcccgtc
     1921 gtgaacggag gggaacctcc gtccgttttg ccgttcccgc aagcgactac gcacgcgacg
     1981 gagaaagaca gaacgggccc gatcaacgtt cttgccatgc gccttttcct agcgcgtaaa
     2041 ccgaagcaac acgagcattt cgtttactcg cgacacgcgg aagtgacttg cccgagcgtt
     2101 cgttctgcgc agaaggaaag gttcgaatca gagcgtgagc agatgaggcg cgtgatccct
     2161 tcgttcgtcg cgtggcggcg attttcgcgc gatttcgtcg atgacatttt cgcgcgcgcg
     2221 cccctccgta ctcgccctga ccgcgggcga ttcacacggc gtgcgcgcag taagactcaa
     2281 acttttcacg cgtcttcgcg ctcagcgccg gcgcgcggac tccgaccgaa tcgaggtgaa
     2341 agctcgcgat ccgacccgtg tcagttagcg ggcgagcgca cgatgagctc gagcgcagag
     2401 gcgaagtgct ggtagtcctc gagtgcattg tagaggtgcg ccgagacgcg gaaagcgcaa
     2461 tcgcggctcc cattcacggt gaaaacgggg acctctatgc gatgctcttc gagcaaccgg
     2521 cgggaaagtg cggccggagc cggtagcccg gcggctggca atgtgacggt tgccatactg
     2581 cccacgaagt cgctgggcgc gctcggaacc gcgcccagtc gctcgccgag caacgcgcga
     2641 gcttcgagca cgagggtgcg gttctgctga tacaaagccg caagcccgcc gggcagcgcc
     2701 gttcgaatga aatcgagcgc gtggggcacc gtcaagtacg cggtcggatc ttcggtccca
     2761 acccagtcga actcgagcca ggcgcgcggg cgctcgggcc ggtcggcggt tgccccgtgg
     2821 ctcacgacga gtgggcgcag ggtgcctctt cgcgcggggg tcgtcgcgag aaaagcggct
     2881 cccttgggag cgcaacacca cttgtgaaag ttgccagtgt agtagtcggc cccgaccgcg
     2941 cgcacgctga gcggaagcat ccccggcgcg tgggcgccgt cgaccaggac ggtcacgccg
     3001 cggtcacgca gctcagccac gatgcgctcg atgggcaaga ccaaggcgct cgggctcgtc
     3061 acgtggtcca cgagggcgaa ccgtgtgcgc ggcgtgagga cccggcgaat cgcatcgagg
     3121 gccgcatcag cgtcgctgag tggccacggg agacatgcct cgacgacgcg ggccgacgca
     3181 cgcgcggccc agtattcggc cgcgttgcgg caagctgcgt acccgtgatc agtgaccaac
     3241 agttcgtcgc cggctcggaa tgggaacgac gcaaggaccg tgttcacggc gctggtggca
     3301 ttcctcacga acacgaagtc ctcgggtgcg gcgtcgacga aggctgcggc gctttgccgc
     3361 gcaacgtcca agagcccctc caagtcgccc gccaagaagc gaacgggatc gcgctccagg
     3421 cgctctcggt acgcgagctg cgcgtcgagc acgacccgcg ggcaggctcc aaacgagcca
     3481 tgattcagaa acgtcagcgc gggatccagc aagaactcgg cgacgcgcgc gttgggcatc
     3541 gccaaaactt agccgcattt ggaggcgctg ccgaggctcg aggcagattc aaccggcgct
     3601 cgcggcggcc ccttttggcg agtcgcgagt cgcgtcggcg ctgctcgagc agagctcgac
     3661 cgagaacacc gctccccggg ggctgtcctc gacgcgcaga tcgccgccgt gtgctcgagc
     3721 gatctcgcgc gcgatgctga gcccgagccc ggcgctgtct tgggccgtgc tgcgtgtttc
     3781 gggagaacgg aaaaatggct cgaaaatgcg ctcgcgatcg acctcgggga ctcctggtcc
     3841 ttcatcgctc acgagcagca cggtgcgctc accttctgcc cgcgccgagc agcgaacggc
     3901 gccgcctgct gggctatggc gaatcgcgtt gtcgaccagg ttgcgcagca agcgctggac
     3961 gtcgcccggc cgtcctcgaa cactgagatc agcgagctcg gcttcgagcc gaatgctctt
     4021 ttcgctgatt tgacgagcga gttcgcgcgc cacttcgtcg aacacccgag cgaggggcac
     4081 gggctcgaat tcttgccggg tgtgttcggc gcgagcgagc gtcaagagat cgtccgcgag
     4141 gtgcttcagt cggcgcgtcg cgtacagcgc ctgggttatg gacgctttgt agtcttcggc
     4201 gctgcgctct cggcgcaacg actgctgaag ttcgccgtaa agcgcggcga ggggcgagcg
     4261 gagttcgtgc gaggcgttcg cgatgaagcg ctgctgcgag cgaatcagca cgcccagccg
     4321 ttccgcgact ccgttcaggt cgttcgccca ttgctcgaga tctgggtctg cgtgtcccat
     4381 cggcgcgcgc gcgtagaggt cgccgcgcgc gacgctgtgc agcgtcgtcg cgatgcgccg
     4441 gtgttcccgc gtgaagcgtc gagtcagcca gagggcagag atcaaaacgc agccgatcga
     4501 agcaaagaac gcgagcaaca tcgcgagctc caggaactcg tcgtcgccgt cgagatcgct
     4561 gcgggaggtc gcgagcaaca aggtcgctcg ttcgcgcgtc gcgagcggta cgagcactgc
     4621 tcgcagcgag cggccgcggt agtgcatgtc gaacggcacg ccgactcgct ctttgaagag
     4681 ctcgtccggt gatgggggcg ccgcgtcgaa cggttcggtc gcggcgatta ccttgtcgcc
     4741 cgcgccgtaa atgatgccgt acttgtcgag cggcccgacg tcgttcgcgg cgggaccggg
     4801 gtgcgtcgtc agggcaaagt tgtggagcgg tgcggcgcgc gcctcctgat gcgcgagctc
     4861 cacgagcgcc tgatccaatt ggtgctcttg gaaacggttc aggagcactt ggatgagcgt
     4921 gagcgcggtg gccagtgcga ccgcgacgac gccggtgatg ccgaggctga ttcgaaattc
     4981 gactttcacg gaagacttcg aggactttcg ctgcaaggat agcggcgcag cgtgctcggg
     5041 gcgatattcg cctcgcgatc actcggacga gcctgctccc ggcccagtct gaccaaggcg
     5101 ggcagtctcg ggtggccggt attacggctc cgtaaggtgc tccgggaaaa gatgacgcta
     5161 cgctttggct cgtgcgcatt ctgctcgttg aagacgacct ccagctcggt gagcttctgc
     5221 ggcgaatttt gagcgaagag ggtcacgcgc tcgagtttac gagtagcctc gccgacgcgc
     5281 gacgtgcgct cgaacaacag cgcgtcgacg tcatcgtgct cgatcggatg ttgcccgacg
     5341 gcgacggcct cgagttctgt ggtgcccttc gcagtcaggg ggacgagacg ccgatcttga
     5401 tgctgaccgc gcggaccgaa gtccaagacc gcgtagcggg ccttcgtagc ggcgcggacg
     5461 actacctcgg gaagcccttc gaagtcgaag agctcctcgc ccgcgccgag gcgttggtcc
     5521 ggcgcagcgc aagctcctgg ctgctggagc tcgggccgct tcgcatcgac cgtcgcgctc
     5581 gcaccgtcga agcgggtggc gtccgcgtcg acctcaccgc tcgggagtat gggctgctcg
     5641 agcgcatcgc ggtggggcgc ggcgagcccg tgtcacgcca aacgttgctc gaggacgtgt
     5701 ggaacatgcg tttcgacccc gggaccagca tgctgaacgt tcacgtcagc cgcttgcgcg
     5761 acaagctccg ctcgtttgct tggctcgtgg agacggtgcc gaatttcggt tatcgcctgc
     5821 gctcgacccg ctgaacgagc tctcacgagc tccgcggagt cgagcgcaag ccaagtcaag
     5881 cctaagcggc gatcgaggcc tgggtcggtt cgctcacggc agcgcggctc tcggccgggc
     5941 tcttgagaag cagagacaag atcccgggca gcacgaacaa cgtcaacgcg gtaccgaacg
     6001 ccatgccggc cacgacgacg gtcgcgagcg gttgctgcac ttcggctccc gcgcccgtgg
     6061 ccagcatcat gggtaggaag ccgagcgcgg cgaccgcggt cgtcgtcaac accgcgcgac
     6121 agacgctgcc gcagccctcg accacggccg cgcggagcga agcgccttga tcgattcgac
     6181 ggcgaacctc cgtcgccatg acgacgccat tgagcacgcc gatgccgccg agcgcgatga
     6241 agcccaccgc cgccggcaga ctgaacggca tgtcgcgcag caaaagtccc accatgccgc
     6301 cggttagaga aaggggcacg agcgccatca ccgcgagcgt cgttcgcatg ttttgaaaca
     6361 tggtgaacag catcccgaag atgatcgcga ccacgacggg gattacgatt ttcaggcgcg
     6421 cgctagctcg ctcgaagttc gcgaactggc cgccccattc gattcggtat ccgctcggcg
     6481 tagacacctg gctacggatc acgccttgcg cttcggccac ccaagacacc agatcgcgtc
     6541 cgcgaaggtt cacgtctact cgcagcgcgc gctcgcgatt cttgtggcgt accgcggcca
     6601 cgccgtcgcc ttccgagata tgggtgatgg agctcagggg gatcaggttt cccgtatgcg
     6661 agctcacgaa gagctcgccg agggacgtgg cggtcggggt agcaggcgga tacaacaccc
     6721 ggatcgcaaa gcgccgcagg ccgtcgtaca cgttccccac ctcgatgcct tcacgcgcgg
     6781 cctggatcgc gctgaaggcg tcttcgacgc ggacgccgaa gcgagacatc ttttcgcgat
     6841 cggcgttcac cgtgattgcc ggctggccga aggctctttc gatgcggacg tcgccggtcc
     6901 cctggatccc catgacgagg cgcccgatgt cgttcgtgat ccgcgtcatc tcctccaagt
     6961 cgcggccgaa tacctgaatt tgaacgtcgg cgcgcgagcc gctcaggatc tcgttggtct
     7021 tgtcctcgat gggctgcgac accgagacga acgtacccgg tacttgcgtt tccaccgcgc
     7081 gtttgatctc tgcgctcaag ccgtcgaagt cgaggccgtc gcgccacttc ttcttctcgt
     7141 gcagcttgac gacgatgtcg gtgctgtcgt ttccaaccgt gtccagcgct acctcggcgc
     7201 gtcccgtgag cgcgaccgtg gtcttcacct ccggcagctt gcgcagcacg gcgtgcgtct
     7261 tgacgtcgag ctcacgcgcc atcgggagtg agatgctcgg agcgcggcgg atcgccacga
     7321 ccaagtctcc ctcgaagatg cgcggaacga actcggcgcc ggcgcgggag aagaggtacc
     7381 ccatggcacc cagggcgacc gtcgcgccga taatccaata ggcgcgcgcc ctgagcactc
     7441 cgggcagggc gcgggcgtag gtctggcaaa tccgctcgat ccagtgcgga ccgtgatcct
     7501 tcgccggggg cacgagcgtg acgagcaggg cggggaagaa cagcaccgcg tagatcagcg
     7561 caccgaagag cgcgcaggcc atggtaatcg ccatcggccg gaacatcttg ccttcggcgc
     7621 cctcgagcgt gagcagcggc acgtacacga gcatgatgat ggcgaccgcg aatgcgaccg
     7681 gtcgcgccac gcttgcggcg gcattggcgt acgcttgcgc gcgcgcgtcc gaagcgagct
     7741 gtttggccgc ggtggcagcg atcacggact cgagcaggac gattggccca tccaccagga
     7801 agccgaaatc gatcgcgccg agagacatca agtctccggt tacaccgaac agccgcatgc
     7861 cgatcaacgc gacgctcatg gaggccggca cgccgatcac gactgcgagc gcgccgcgaa
     7921 tgctccccag gaacaacgcg agcacgagca gaacgatcgc cacgccttcg gcgaggttcg
     7981 tcatcaccgt gcggatggtg ctgccaacga attcggcgcg gtcgtagtag gcctcgatgc
     8041 tcactcccgg cggcagcgtc ttggcgattt cttgcacgcg ttcgcccacc gcgtgcacta
     8101 cgtcgcggct gttggcgccc atcagcatca tcaccgtgcc gctcaccacg tccgcgtcgc
     8161 cttgc
//
LOCUS       DASTKS010001034         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1280795, whole genome shotgun sequence.
ACCESSION   DASTKS010001034 DASTKS010000000
VERSION     DASTKS010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280795"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..2066
                     /gene="istA"
                     /locus_tag="VFQ35_21865"
     CDS             369..2066
                     /gene="istA"
                     /locus_tag="VFQ35_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9933373.1"
                     /translation="MRKVREVLRLHFECGRKQREIARSCAIGAGTVSDYLKRARDAGL
                     DWKTASELSETELENRLFRYGQRPTTGERAAIDFGLVHQELKRTGVTLQLLWVEYQQA
                     VRARGDGIEPYQYSQFCELYRNWRGKLGISLRQVHRAGEKAFIDYSGKRPRIVDSATG
                     EIRDVELFVMTLGASNYTYAEATLTQKLADFVASTVRGFEFFGGVPEVVVPDQLRSAV
                     SGPDRYDPDINGTYLEMAKHYGVAVVPARPGKPRDKAKVESAVLVAQRWILARLRNRS
                     FFSLDDLNRAISELVSELNERPFQKLDGSRKTAFESLDRPALRALPASRYELGVWTRA
                     KVHIDYHVAFDHRFYSVPVALVGVRVEVRATRSVVEIFSRGVRVASHPRSYAPKGTAV
                     TNDEHRPHAHREYGKWPPARLVAWASTLGPNIALVVERMLGRHSRPELAYRPVLGVIR
                     LGDRYGTARLERACERALTASHLDGARYRYIESILRLGLEGAPLEPKEPAQTLPTAHE
                     NIRGGDYYETEDTSDHGRNDPEDARAQAHDDGRSFSTTAREPTRAATIVGREARTTH"
     gene            1984..2700
                     /gene="istB"
                     /locus_tag="VFQ35_21870"
     CDS             1984..2700
                     /gene="istB"
                     /locus_tag="VFQ35_21870"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9933374.1"
                     /translation="MAEAFRQLLASPPEQQLSWEEKLGLLIDREWTDRENRRLARRLR
                     EAHLGVRASLEDVVCEPGRGIDKPQLRQLGSCQWVKAHQNVILLGPTGVGKSYLASAL
                     AEAACRRGARAFFMRVPRLTEELAVARASGAYGATLARFAKADVLVLDDFLLAPMKDS
                     ERRDLLEVLEDRYDKSSTIITSQLPTKTWHEALGDPTIADAICDRLVHNAHVVTLRGH
                     SMRRRKGASQPATEVSTPNP"
BASE COUNT          572 a          910 c          886 g          504 t
ORIGIN      
        1 ggccttgcaa gatcgttggg ccgaacacgt gcgcgaagtt catgcgcacg gactgtctcc
       61 cgacaacgtg gtccacttga tggatcgtga aggcgacgac tacgaggtgt tggatcttgc
      121 gtattccgac ctgactcgag cagctgttcc gagctgacct gagcgccgat tccggagcac
      181 ctgagcacgg attccgagcc actcgagcag ccgttccgga acacctgagc acggattccg
      241 agccactcga gcaccgattc cggatccgag cggccgcgtg gccaggctcg gagccagcga
      301 cgtcgttcac tgcgctttgg agcgccgttc tgcgcggcga ccaagcgtgg atgtccgagc
      361 gagtcagcat gcgcaaagtt cgagaggttc tgcgactgca cttcgaatgc ggtcggaagc
      421 aacgtgagat tgcaaggtcg tgcgcaattg gcgccggcac cgtcagcgac tacctgaagc
      481 gagctcggga cgctgggctc gattggaaga cagcgagcga gctgagcgag accgagcttg
      541 agaatcgctt gtttcgctac gggcagcggc ccacgacggg agagcgcgcg gcgatcgatt
      601 tcgggctcgt gcatcaggag ctgaagcgca cgggcgtgac gttgcagctc ttgtgggtcg
      661 agtaccaaca agccgtgcgg gctcgcggtg acggtatcga gccatatcag tacagtcagt
      721 tctgcgagct ctatcggaat tggcgcggga agctcggcat ctcattgcgc caagtgcata
      781 gagccggcga gaaggcgttt atcgactact ccggcaaaag gccccgcatt gtcgacagcg
      841 caaccggtga gatccgcgat gtagagcttt tcgtgatgac gctcggcgcg tccaactaca
      901 cgtatgcgga ggcgacgttg acgcaaaagc tcgctgactt cgtcgcctcg acggtgcgcg
      961 gtttcgaatt tttcggtgga gtaccagagg tagtggtacc cgatcagctg cgcagcgccg
     1021 tgagcggacc cgaccgctac gatccggaca tcaacggcac gtatctggag atggccaagc
     1081 actacggcgt cgccgtggtg ccggcgcgtc ccggcaagcc gcgcgacaag gcgaaggtcg
     1141 agagcgccgt gctggtcgcg caacgttgga tattggccag gctccgcaac cgcagcttct
     1201 tcagtctgga cgacttgaat cgagccatat ccgagctcgt tagcgagctc aatgagcgcc
     1261 catttcaaaa gctggacggc tctcggaaaa ccgccttcga gagcctcgac cgaccggcgt
     1321 tgcgagcgtt gccggcgagc cgctacgagc tcggcgtttg gacgcgagcc aaggtccaca
     1381 tcgactacca tgtggctttc gatcaccgct tctacagcgt gccggtcgcg ctcgttggtg
     1441 tgcgcgtcga ggtgcgcgca acgcgctcgg tcgtggagat attctcacgg ggcgtgcgcg
     1501 tcgcgtcgca cccgcgcagc tacgcgccca aagggacggc cgtaacgaac gatgagcatc
     1561 gaccgcatgc acaccgtgaa tacggtaagt ggccacctgc acgcttggtg gcttgggcga
     1621 gcacgctcgg ccccaacatc gcgctcgtcg tcgagcgtat gctgggtcga cactcacgcc
     1681 cggagctcgc ctatcgcccg gtgctcggcg tgatccgatt gggtgatcgc tacggcaccg
     1741 cgcgcctgga gcgggcctgc gagcgcgccc tgacggcgtc tcatctagat ggcgctcgct
     1801 atcgctacat cgaatcgatt ctcaggctcg gcttggaagg cgcgccgctc gagccgaaag
     1861 agcccgctca aacactaccc accgcccacg aaaacatccg cggcggcgac tactacgaaa
     1921 cggaggacac gagtgatcac ggaagaaacg atccagaaga tgcacgagct caagctcacg
     1981 acgatggccg aagcttttcg acaactgctc gcgagcccac ccgagcagca actatcgtgg
     2041 gaagagaagc tcggactact cattgaccgc gaatggactg accgagaaaa tcgccgtctc
     2101 gctcgtcgct tgcgcgaagc acaccttggc gtgcgggcat cacttgaaga cgtcgtctgc
     2161 gagccaggtc gaggaatcga caagccccaa cttcgtcaac tcggctcctg ccagtgggtg
     2221 aaggcgcacc agaacgtgat tctcctcggg cctaccggcg tcggaaaaag ctacctggcc
     2281 tccgcgctag ccgaagccgc gtgccgcaga ggcgctcgtg ccttcttcat gcgcgtacca
     2341 cgcctcaccg aagagctggc ggtcgctcgc gcctcgggcg cctacggcgc tacactcgcg
     2401 cgcttcgcca aagccgacgt gctcgtgctc gacgacttcc tactcgcgcc catgaaggac
     2461 tctgagcgtc gcgacctgct cgaagtgctc gaggaccgct acgacaagag ctccacgatc
     2521 atcacatctc aattgccgac gaaaacttgg catgaagccc tgggtgatcc gacaatcgcc
     2581 gacgccatct gcgaccgcct cgtgcacaac gcccacgtgg tcacgctgcg cggtcactcc
     2641 atgcgccgcc gcaaaggcgc ttctcaaccc gcaaccgagg tctcaacccc caatccctga
     2701 aaccagacgt cgcttcgctc cgagcctgct cgagcccgtc ggaactgacg ctcgggtcct
     2761 gccggaatga ctgctcgagt gcagcggaat acgcaggatc tgctgaaacg tttgcagact
     2821 cgcttcgtac tacgagtcca gcacaatcga gttctgaaga acgggacgct tc
//
LOCUS       DASTKS010001035         6030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_386334, whole genome shotgun sequence.
ACCESSION   DASTKS010001035 DASTKS010000000
VERSION     DASTKS010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386334"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFQ35_21875"
     CDS             complement(<1..1247)
                     /locus_tag="VFQ35_21875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933375.1"
                     /translation="MDDYMDVRPGEVLAGKYRIEQVLGQGGMGVIVQATHLQLDERVA
                     IKFLLPQALENEEAVMRFAREARAAVKIKSEHVARVTDVGTLGTGCPYMVMEYLSGSD
                     LSQLIQDRGPLPIEEAVDYILQASEALAEAHALGIVHRDLKPANLFHTRRADGSSCIK
                     VLDFGISKVTGLSGSGADLGMTRTTAVMGSPLYMSPEQMASSRNVDARTDIWSLGIIL
                     YELLAGRAPFLADTMPQLCAMILQQSAPPLRNVRPDAPEALQSVLARCLEKEPARRYA
                     NIAELAAALLPFASRLGKNSVERISRVMSAAGISSREVNVPAQALRTVSDGTQSGWGN
                     TAQTKKGRGPWLLLGAALLALGGAAAALLVTRSLNAPEKTPTSAAEPAAPTAPAPTAE
                     GTAPSLAKSPEAPAVAIAVPAAS"
     gene            complement(1474..2685)
                     /gene="hemW"
                     /locus_tag="VFQ35_21880"
     CDS             complement(1474..2685)
                     /gene="hemW"
                     /locus_tag="VFQ35_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET9933376.1"
                     /translation="MTSETFAAVPTETLGVYVHFPWCLKKCPYCDFLSLPAARDEIPH
                     ARYADAVIAELAARRASLPDLPLRSIFFGGGTPSLWKADELGRVLARILEAFPVHGPN
                     LEITAECNPSSFDDTVATGLARAGVNRISLGVQSLDRERLAFLGRLHDAAGALEALDV
                     ATHHPFQRVSADLIFGVAGESPEAAKQEVETLVERGITHLSAYALTIEPGTQFGELAR
                     RNRLPLLSDDAVADSFAAVEGALEARGFVHYEVSNYAKPGAEAQHNLGYWRGEPYVGI
                     GAGAWGTLPIRDARLRYRNTPSAERYLGWAREPSGVDLEREGEFVAVTELLDGETAFR
                     ERLMLGLRLAEGFDWRGSEARTGATMWTAARRRAFEKWSRRGALEERDGRLHIERRHW
                     LLADGIIADLM"
     gene            2914..3699
                     /locus_tag="VFQ35_21885"
     CDS             2914..3699
                     /locus_tag="VFQ35_21885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9933377.1"
                     /translation="MKRLHLFEFEDLPWFPQVVREAMTDYLAFMGAVEKPYLEFVELL
                     ARALDRTGDDTLLDLCSGGGGPALPLIRLLSRKRSAPTRVVLTDLYPNRPRFDLAKRQ
                     GNGLVDCIDMPVDATSVPEGLGGFRLMSNSFHHLPPSVARACLADAAQKGRGIAVCEL
                     VNRSPGSVLQIGIGTLTMFAVTPFIRPFRWSRLLLTYALPIVPLCTLWDGVVSCLRAY
                     DVGELRELTEGLGGEGYHWEIGRLPIPRTPAAITYLIGTPASA"
     gene            3734..4561
                     /gene="dapF"
                     /locus_tag="VFQ35_21890"
     CDS             3734..4561
                     /gene="dapF"
                     /locus_tag="VFQ35_21890"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9933378.1"
                     /translation="MKFWKAHGLGNDYLVLEAALALDADLVRDICDRHRGLGGDGILE
                     PKPSREGADYGVRIWNPDGSIAEKSGNGLRIFARWLHDERAAPESFSVSTDACVVGCK
                     ATADSVSVEMGRPSFAPDDVPVVAEGELIDGPIELASVTLTATAVGMGNPHCVIFRNE
                     PLDSLPWRKWGAELERHPRFPNRTNVQVARVIEEHEVEIRIFERGAGETQASGSSSCA
                     VAAAGVRTGRLRAGRIRVRMPGGVLYVTVSDDGILLEGPVEGIGRFELDSLWLRARS"
     gene            complement(4563..5645)
                     /locus_tag="VFQ35_21895"
     CDS             complement(4563..5645)
                     /locus_tag="VFQ35_21895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET9933379.1"
                     /translation="MSSGLQIKENGALDFVSLGALVHRLDPGIVPFRKATECQIHVSG
                     GEFNVAANLSDCFRLNTAIVTAMVDYPIGDLIAERVRAMGVRPVYKTFKHNGVNGPNM
                     ATVYSDRGYGMRPPVVFYNRSNEAAALLKPGDVDWSALFANGVRWFHSGGIFASLSQT
                     TGELIIEGMRAAKKAGAIVSFDLNFREKLWNVWGGRQTASNVIGHIAEYVDVLIGNEE
                     DLQRGLGIVGPEVSHRSKLEPSAFFAMMDQVVARYPQVKAVATTLREVHSTNRHTWGA
                     VAWVAGETHTAPPLELDVLDRVGGGDGFAAGFIYGLLSGETPEAALRLGWAHGALLTT
                     YPGDTTMATLEQVRALAGGGSARIQR"
     gene            complement(5825..>6030)
                     /locus_tag="VFQ35_21900"
     CDS             complement(5825..>6030)
                     /locus_tag="VFQ35_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933380.1"
                     /translation="SFSQCMSDCMSIVDGFPECAAEALAMNQCYAGTPSGEDHWLCNG
                     DGFMPSSFDCFDQEMAFYSCLGF"
BASE COUNT          993 a         1931 c         2039 g         1067 t
ORIGIN      
        1 ttcgacgccg cgggcaccgc gatcgcgacg gctggtgcct ctggcgattt cgcgagcgac
       61 ggagccgtgc cttccgccgt gggcgccggt gccgttggcg cggccggctc agcggcggag
      121 gtcggagttt tttcgggcgc gttcagcgaa cgggtgacca gcagggccgc tgctgcgccg
      181 ccgagcgcga gcaacgcggc gccgaggagc aaccaaggcc cgcggccctt cttggtctga
      241 gcggtgttgc cccagccaga ctgggtcccg tcgctgacgg tgcggagagc ttgtgcaggt
      301 acgttgacct ctctgctcga aatccctgcc gccgacatga ctcgcgaaat gcgctcgacc
      361 gagtttttgc cgagccgaga agcgaacgga aggagcgcgg cggcgagctc ggcgatgttg
      421 gcgtaacggc gcgcgggctc cttctccaag caccgcgcga gcaccgattg gagcgcctcg
      481 ggcgcgtcgg ggcgcacgtt gcggagcggc ggcgccgact gctgcaaaat catggcgcag
      541 agctggggca tcgtgtccgc gaggaacggt gcgcgccccg cgaggagctc gtagagaatg
      601 atgccgagtg accagatgtc ggtccgcgca tcgacgttgc gcgaagaggc catctgctcg
      661 ggcgacatgt agagcggaga gcccatgacc gcggtcgtgc gcgtcattcc caagtccgcg
      721 ccagaaccgc tcagccccgt caccttggag atgccgaagt cgagcacctt gatgcaggag
      781 ctcccgtccg cgcgccgggt gtggaacagg ttcgccggct tcaagtcgcg atgaacgatg
      841 ccgagcgcgt gcgcctctgc gagcgcctcc gatgcttgga ggatgtagtc gacggcctct
      901 tcgatgggca gtggcccgcg atcttggatg agctggctca gatcgctccc cgacaggtac
      961 tccatgacca tgtacggaca gccggtgccg agcgttccga cgtcggtgac gcgcgcgacg
     1021 tgctcgctct tgatcttcac cgctgcgcgc gcctctcgcg cgaagcgcat gaccgcctct
     1081 tcgttctcga gcgcctgagg cagcaagaac ttgatcgcga ctcgctcgtc gagctgcaga
     1141 tgcgtggcct ggacgatgac gcccatgcct ccctgaccga gcacttgctc gattcggtac
     1201 ttacctgcga gtacttcgcc gggccgaacg tccatgtagt cgtccactga tacgatccga
     1261 cagcgcctat cttacggtcg cgtggaccca ctacctgcgc tgtcgcccag actagtgcaa
     1321 ctttcgccag gtggggcgcc ggcagttttg ggaatccaat ctcttggggg tgctgacccg
     1381 cagggtccgg cgcgcccgcg gccccggggc aacgtttggg cttatctatt tgaatttact
     1441 tgtaaatatg tttggtgcgc cagagggcgg cgactacatc agatccgcga tgatgccgtc
     1501 ggccaggagc caatgccggc gctcgatgtg caacctgccg tcccgttcct ccaacgcgcc
     1561 gcgccgcgac catttctcga acgcgcgccg gcgagcagcg gtccacatcg tcgcgccggt
     1621 ccgagcctcg ctgccacgcc agtcgaagcc ctcggccaat ctgagcccga gcatgagccg
     1681 ctctcgaaac gccgtttcgc cgtcgagtag ctcggtcacg gcgacgaact cgccttctcg
     1741 ctcgagatcc acgccggacg gctcgcgcgc ccagccgagg tagcgctcgg cagaaggggt
     1801 gtttctatag cgcaagcgtg cgtcgcgaat cgggagcgtt ccccacgcgc cggctccgat
     1861 ccccacgtag ggttcgccgc gccagtaccc gaggttgtgc tgcgcctcgg ctccgggctt
     1921 cgcatagttg ctgacctcgt agtggacgaa gccgcgcgcc tcgagtgcgc cttcgactgc
     1981 cgcgaacgag tctgcaaccg cgtcgtcgga gagcagcgga aggcgattgc gccgggctag
     2041 ctcgccgaac tgggtgcccg gctcgatggt gagagcgtac gcgctcagat gcgtgatgcc
     2101 gcgctcgacg agggtttcca cttcctgctt ggcggcttcc ggagactcac ccgcgacgcc
     2161 gaaaatcaag tccgccgaga cgcgctgaaa agggtggtgg gtggcgacgt cgagcgcttc
     2221 gagcgcgccg gccgcgtcgt ggagccgccc gaggaacgct agccgttctc ggtcgagcga
     2281 ctgtacgccg aggctgatgc gattgactcc ggcacgtgcg aggcccgtag ccaccgtatc
     2341 gtcgaagctc gacgggttgc actcggccgt gatctcgagg ttcggtccgt gcaccgggaa
     2401 ggcttcgaga attcgagcga ggacgcgacc gagctcgtcg gctttccaga gggaaggcgt
     2461 accgccgccg aaaaagatgc tgcgcagagg caagtcgggc aaactcgccc ggcgcgcggc
     2521 gagctccgcg atgaccgcgt cggcgtaacg agcgtgaggg atctcatcgc gcgctgcggg
     2581 gaggctcaag aagtcgcaat aagggcactt cttgaggcac caggggaagt ggacgtagac
     2641 gccgagcgtt tcggtcggta cggcggcgaa agtctcgctc gtcatccgtt ggctttcagc
     2701 gtcacgtcga agggagcagc ctttggggtt tcggggggca cagtgggcac gggacgtgag
     2761 actgacccat ctcagcgcgg gctaccaacc aaaccccgag aaacttggcc gcggagccga
     2821 tggtttcttg tgcgtaattc gggcagtgca ggcgagaagt gttacgcgtt ttcggcgaag
     2881 tcgtggagaa tccgccctgc gcgagggccc tgcatgaagc gccttcatct tttcgagttc
     2941 gaagacctcc catggttccc gcaagtcgtg cgcgaagcga tgaccgacta cctcgcgttc
     3001 atgggagcgg tcgagaagcc ctatctcgag ttcgtcgagc tgctcgcgcg ggcgctcgat
     3061 cgaaccggtg acgatacgct cctcgatttg tgctcgggcg gcggcgggcc ggctttgccg
     3121 ctgatccgcc tgttgagccg aaagcgcagc gcgccgactc gggtcgtgct caccgatttg
     3181 tatcccaacc gtccccgctt cgatttggcg aagcgccagg gcaacgggct cgtcgactgc
     3241 atcgacatgc cggtcgatgc gacgagcgtc cccgagggcc tcgggggctt tcgcttgatg
     3301 agcaactcgt ttcaccatct tccgccgagc gtggcgcgcg cttgcctcgc tgacgccgcg
     3361 caaaagggcc gaggcatcgc cgtgtgcgag ctcgtgaatc gttcgccggg gagcgtgctt
     3421 cagatcggca tcgggacgct gaccatgttc gcggtgacgc cgttcatacg accgtttcga
     3481 tggagccggt tgctgctcac gtacgcactc ccgatcgttc cgctctgcac gctctgggac
     3541 ggcgtggttt cttgcctgcg cgcctacgac gtgggtgagc tgcgtgagct aacggaaggt
     3601 cttggaggtg aggggtacca ctgggaaatc gggcggttac ccatcccgcg cacgcccgca
     3661 gcgatcacgt acctgatagg cactcccgcg tcggcgtgag tcgtcttcga cgaggcgtga
     3721 taagagtccc ggcgtgaaat tctggaaggc gcacggactc ggaaacgact acttggtgct
     3781 cgaagcggct ctcgccctcg acgcggatct cgtccgggac atttgcgatc ggcaccgtgg
     3841 cctcggcggt gacggcatcc tcgagcccaa gccttcgcgc gagggcgccg actacggcgt
     3901 tcgcatctgg aacccggacg gatccatcgc cgagaagtcg ggcaacgggc tccgcatctt
     3961 cgcgcgttgg ctgcacgacg agcgcgctgc tcccgagtcg ttcagcgtct cgaccgatgc
     4021 ttgcgtcgtc gggtgcaagg cgacagccga ctcggtcagc gtcgaaatgg gtcgcccgtc
     4081 gttcgcgccg gacgacgtcc cggtggttgc ggaaggtgaa ctcatcgacg gcccgatcga
     4141 gctcgcgagc gtgacgctga ccgcgacggc ggtgggcatg ggcaacccgc attgcgtgat
     4201 cttccgcaac gaaccgctcg attcgttgcc ctggcgcaaa tggggcgccg agctcgagcg
     4261 ccacccgcga ttcccgaacc gcaccaacgt gcaggtggcg cgcgtcatcg aggagcacga
     4321 ggtcgagatt cggatcttcg agcgcggtgc gggcgaaact caagcttccg gctcgtcgtc
     4381 gtgcgcggtc gctgcggctg gcgtgcgcac tgggcggctg cgcgccggcc gcattcgagt
     4441 gcgcatgccg ggcggggtgc tctacgtaac cgtatcggac gacggcatct tgctcgaagg
     4501 gcccgtcgaa ggcatcggcc gcttcgagct cgactccctc tggctgcggg cgcgcagttg
     4561 attcagcgtt ggatgcgggc ggagccgccg ccagcgagcg ctcgcacttg ctcgagcgtc
     4621 gccatggtgg tgtcgcccgg gtaggtcgtg agcagcgcgc cgtgcgccca gccgagccgc
     4681 agcgccgctt ccggcgtctc gccgctgagg aggccgtaga tgaagcccgc cgcgaagccg
     4741 tcgccgccgc ccacgcgatc gagcacgtcg agttcgagcg gaggcgcggt gtgggtctcg
     4801 cccgcgaccc aggcgaccgc gccccaggtg tggcgattcg tggagtgcac ttcgcgcagc
     4861 gtcgtcgcga ccgccttcac ttgcgggtaa cgcgcaacga cctggtccat catcgcgaag
     4921 aaggcggagg gctcgagctt cgagcgatgc gagacctctg gacccacgat gccgaggccg
     4981 cgctgaagat cctcttcgtt gccgatgagc acgtccacgt actcggcgat gtgcccgatg
     5041 acgttgctgg cggtttgtcg cccgccccac acgttccaca gcttctcgcg gaagttcagg
     5101 tcgaacgaga cgatggcgcc cgccttcttc gcggctcgca ttccttcgat gatgagttca
     5161 ccggtcgtct gagaaagcga agcgaagatg ccgccgctgt gaaaccagcg cacgccgttc
     5221 gcgaagagcg cgctccaatc cacgtcgcca ggcttgagca gcgcggcagc ctcgttcgag
     5281 cgattgtaga agacgacggg cggccgcatg ccatatccgc gatcgctgta aacggtcgcc
     5341 atgttggggc cattcacgcc gttgtgcttg aacgtcttgt agacggggcg cacccccatc
     5401 gcgcgcacgc gctccgcgat gagatcgccg atggggtagt cgaccatcgc ggtgacgatc
     5461 gcggtgttca gccgaaagca gtcggacaag ttcgcggcga cgttgaactc cccgccgctc
     5521 acgtggattt gacactcggt cgctttgcgg aacgggacga tgccaggatc gagccgatgg
     5581 accagggcgc cgagggagac gaaatcgagg gcgccgttct ctttgatctg aagaccgcta
     5641 gacatggctc acgttcccct cgtttccctc gcgaacgctt cgcggacagg gaaagccgag
     5701 agcaggtttt ccggaaaaat cgctcgcgtg ctcctagcac gaaccccaca aaaagaaacg
     5761 cgggcggagc ttggcgactc cacccgcgga acaaacctgc tcgtggggtt gaacgctcgg
     5821 cgctttagaa cccgaggcag ctgtagaagg ccatctcttg gtcgaagcag tcgaacgagc
     5881 tcggcatgaa gccgtcgccg ttacagagcc agtggtcctc gccggacgga gtgcccgcgt
     5941 agcactggtt catggcgagg gcctcggccg cgcactctgg gaacccgtcc acgatgctca
     6001 tgcagtcgct catgcactgc gagaagctcg
//
LOCUS       DASTKS010001036         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1726997, whole genome shotgun sequence.
ACCESSION   DASTKS010001036 DASTKS010000000
VERSION     DASTKS010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726997"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFQ35_21905"
     CDS             <1..272
                     /locus_tag="VFQ35_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933381.1"
                     /translation="SVAHGVRRAKIEIAVYSLLLVVVSLLPVGLGLVGLGYAVVALGC
                     GLGFLALATAGLSMSDDRRWARRVFFASMPYLVVVLGALAAFSVR"
     gene            complement(375..788)
                     /locus_tag="VFQ35_21910"
     CDS             complement(375..788)
                     /locus_tag="VFQ35_21910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933382.1"
                     /translation="MEASPLHVLVLEDDPMVRRGLSRALRVAGHDVVAVETCGNVRAL
                     DAGFDVAILDLELPDGSGVEVASELLALGATQGVVFFSGAGDPVLLSRAERFGEVVAK
                     SENLAPLLEAIHSSRHRDPRRARRTRPVAPRTAWR"
     gene            complement(859..1890)
                     /locus_tag="VFQ35_21915"
     CDS             complement(859..1890)
                     /locus_tag="VFQ35_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933383.1"
                     /translation="MNAAVPEQARPTEARAPAVDTSSSVTASGFAALSLCAILAARGL
                     APALPGTATGIARLIGATVWFAACVSQLVAAGGIALSVRLLGVVFSLPSLGIAFRFVG
                     LGAGLSVITLVAASATRPLDGELGKLLALAAWILPACTLPFVFSRRALRPMALAVSLA
                     VLASGVDLVAYEAARRATASDGLLGPISLALSMLSLMADAATAVFSARALSSSFRRAR
                     TPIVIALALTVLALVLARVGSSHTAPTLLVLVQRFVEALSPERPAISDALQATVAVFS
                     LMSVAALLVLRRGRHDVRAALSLMLLARTCAPTPIAALLSITSALLIARAVNDPHLVP
                     ERETAKSMN"
     gene            complement(1894..>3671)
                     /locus_tag="VFQ35_21920"
     CDS             complement(1894..>3671)
                     /locus_tag="VFQ35_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933384.1"
                     /translation="LPRDAHIPLFLWIATALLVHLLGGGGVNKAADWIEERVELRRFA
                     EGVRSYMKSQNTLEVALLDETQPAPDAPDEKRPDVDPDKKPPEPEKSDDEDDKKPLPP
                     ELDQKKEPEKPEPKKPEEPEKEKKPEEPKKPVEVVEKQPDKAAQELMLKKSIAVRQHV
                     EDEKQPDNPDAKFISEHNNKVAEESQARITSNDQDDKKPTPGSNASGVGEEPGDSHET
                     RIAQSEDKPGEEARAPHDAPAQSHPVVSKEQPNAGAPRAPSPEHVAKAQSGAENGGEK
                     LTEQRTPATPSPGSKGQAEQREQLGAPETLDSNQGTFGVARPQMAQAEQEGQRARKKR
                     ELPRRRVRGAEDFLGLGASGTTASGINLNLTPGLAASAIGTDTLARERLADGARRRSQ
                     HRGSWKSVGIERWRSAIENYVPMVKPGNQTALNTARSPFGTYLASIHNRLHPIFADWF
                     LASLDSLPASDPQNNHEIFTSLEIVLDQDEGRVVKEGVTRTSGVTAFDISALESVRRA
                     APFGTPPREIVSGDGNVYLHWEFHRDPAIACTTYNARPYILKGLQKAAPPPPEPIKPT
                     PDQNPEQERHGLRETPRDSANERSL"
BASE COUNT          555 a         1184 c         1251 g          681 t
ORIGIN      
        1 tatcggtcgc gcacggcgta cgccgcgcca agatcgagat agcggtgtac tcgctgctgc
       61 tcgtcgtcgt gagtctgctg ccggttggcc tcggcctcgt cgggctcggt tacgccgtgg
      121 tcgcgctggg gtgcgggctc ggatttctcg cgttggcgac ggctgggcta tcgatgagcg
      181 acgaccgacg ctgggctcgt cgcgtgttct tcgcatcgat gccgtacctc gtcgtcgtac
      241 tcggcgcgct cgcggccttc tccgtgcgct gagtaagttc cgaagcaggc tcgctgccga
      301 cacagggcga cgctccccgc tctcgcttcg agcgctttcg cgcgaggtct cagtgagctt
      361 tcgcgcgagg tctctcaccg ccaggcggtg cgcggcgcga cgggacgtgt gcgtcgagcg
      421 cgcctcggat ctcgatgacg cgagctgtga atggcctcga gcagcggcgc aaggttctcg
      481 ctcttggcca cgacctctcc gaagcgctcc gcgcgactca agaggacggg gtcgcccgcg
      541 ccgctgaaga agacgacgcc ttgcgtggcg ccgagcgcta ggagctcact cgctacttcg
      601 acgccgctgc catccggcaa ctcgaggtcg aggatcgcca cgtcgaaacc ggcatcgagc
      661 gcgcgcacgt tgccgcacgt ctccaccgca accacgtcgt gtcccgccac ccggagcgct
      721 cgcgagagtc cccgtcgtac catcgggtcg tcctcgagga cgagcacatg caacggagaa
      781 gcctccatcg agcgcgctca acatatcagt agatctgctc ctgaacaagt gaatcgcagt
      841 cgatactcac ccagcgaatc aattcatgga cttcgctgtt tctcgttcag gtacgaggtg
      901 aggatcgttc actgcacggg cgatcaagag cgcgctcgtg atcgagagca acgctgcaat
      961 cggtgtgggc gcacacgtgc gtgcgagtag catcagcgat aacgccgcgc gcacatcgtg
     1021 tcgtccgcgc cgcagcacga gcaaagccgc gacgctcatc aacgagaaca cggcgaccgt
     1081 agcttgcagc gcgtcggata tcgccggacg ttctgggctc aaagcctcga cgaagcgctg
     1141 gacgagcacg agcaacgtcg gcgcagtgtg actcgagccg acacgcgcga gcaccaaagc
     1201 cagcaccgtg agcgccaacg caatgacgat cggcgtgcgc gctcggcgaa agctcgatga
     1261 cagcgcgcgc gccgagaaga cggcggtcgc tgcgtcagcc atcagggaaa gcatcgagag
     1321 cgcgagcgag attgggccga gcaagccatc gctcgcggtc gcgcggcgcg cggcttcgta
     1381 cgcgaccaga tcgactccgc tcgcgagtac ggcgagagat actgcgagcg ccatgggccg
     1441 cagcgcgcgc cgagagaaca cgaagggtag ggtgcacgcc gggaggatcc acgccgcgag
     1501 cgccagcaac ttgccgagct ctccgtcgag cgggcgcgtc gccgacgcgg caacgagcgt
     1561 tatcacgctg agccccgcgc cgagcccgac gaagcgaaag gcaataccta ggctcggtag
     1621 agagaatacg acgcccagta gacgcaccga gagcgcgatg cctcccgccg cgacgagctg
     1681 actcacacac gccgcaaacc acacggtcgc gccaatcaac cgagcgatgc cggttgcggt
     1741 gcccggcaac gctggcgcaa gcccccgtgc agcgaggatg gcgcacagcg aaagcgctgc
     1801 aaagccgctc gccgtgaccg aagacgaggt gtcgaccgcg ggcgcacgtg cctccgtcgg
     1861 gcgcgcttgc tcgggaacag cggcgttcat gctctacaga ctacgctcgt tcgccgaatc
     1921 gcgtggagtt tcacgcaggc cgtgccgctc ttgttccggg ttttggtctg gcgtcggttt
     1981 gatcggctcg gggggagggg gcgcggcctt ctgcaacccc ttcaagatgt aggggcgagc
     2041 gttgtaagtg gtgcacgcga tggccggatc gcggtggaac tcccagtgca ggtacacgtt
     2101 gccgtcgccg gaaacgattt cgcgcggggg ggtgccgaac ggggccgcgc ggcgcaccga
     2161 ctcgagcgcg ctgatgtcga aggcggtgac gccgctcgtt cgcgtgacgc cttctttcac
     2221 gactcggccc tcgtcttgat cgaggacgat ctcgaggctc gtgaagatct cgtgattgtt
     2281 ttgagggtcg ctcgcgggca gcgagtcgag gctcgccaag aaccagtctg cgaagatggg
     2341 atggagacgg ttgtggatgg acgcgagata cgtcccgaag ggtgagcggg cggtattcag
     2401 cgcggtctgg ttgcctggct tcaccatcgg tacgtagttt tcgatggcgg agcgccagcg
     2461 ctcgatgccg accgacttcc aggagccgcg gtgctggctg cggcgacgag cgccgtccgc
     2521 caagcgctcg cgcgccagcg tatccgtgcc gatcgcggac gcagcgagcc ccggggtcaa
     2581 gttcaggttg atgccgctcg cggtggtgcc cgaggctccg agccccagga agtcttcggc
     2641 gccgcgcact cggcgacggg gcagctcgcg cttcttgcgg gcgcgttgcc cctcctgctc
     2701 ggcttgagcc atttgcggcc gagcgacgcc gaacgtgcct tgattcgagt cgagggtttc
     2761 gggcgcgccg agctgctcgc gctgttcggc ttggcccttc gagcccgggg acggtgtcgc
     2821 gggcgtgcgc tgttcggtga gcttctcgcc gccgttttcg gcgccgctct gcgccttggc
     2881 gacgtgctcg ggtgaggggg ctcgaggtgc gccggcgttc ggttgctcct tgctgaccac
     2941 cgggtggctc tgcgcgggcg cgtcgtgagg ggcgcgcgct tcttcgcccg gtttgtcctc
     3001 actctgggcg atgcgggttt cgtgcgagtc gcctggctct tcgccgacgc cgctcgcgtt
     3061 cgaaccgggc gtcggcttct tgtcgtcctg atcgttggac gtaatccgcg cctgactctc
     3121 ctcggcgacc ttgttgttgt gctcggagat gaacttggcg tcggggttgt cgggctgctt
     3181 ttcgtcctcc acgtgctgcc gcactgcgat ggacttcttc agcatgagct cttgagctgc
     3241 cttgtcgggc tgcttttcga cgacctcgac cggcttcttg ggctcctctg gcttcttctc
     3301 tttctccggc tcctcaggct tcttcggctc cggcttctcg ggctctttct tctggtcgag
     3361 ctcgggaggc aggggcttct tgtcgtcctc gtcgtccgac ttctccggtt caggaggctt
     3421 cttgtctggg tcgacgtcgg gcctcttctc gtcgggggcg tcaggagcag gctgcgtctc
     3481 gtcgagtagc gccacctcga gagtgttctg gctcttcatg tacgagcgca caccctcggc
     3541 gaagcgacga agttcgacgc gctcctcgat ccagtccgcg gccttgttga cgccaccgcc
     3601 cccgagcaaa tgcacgagca acgccgtggc gatccacagg aagagcggaa tgtgtgcgtc
     3661 gcgcggcagc t
//
LOCUS       DASTKS010001037         6450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_610157, whole genome shotgun sequence.
ACCESSION   DASTKS010001037 DASTKS010000000
VERSION     DASTKS010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610157"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFQ35_21925"
     CDS             complement(<1..655)
                     /locus_tag="VFQ35_21925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933385.1"
                     /translation="MSELRQSTPLAPERPGSLSGESPSGPATLLNSRQCPRCGGRFPA
                     DFKVCPRDAVPLEDAVSDEDAMVGQLLSGTYEIVRVIGEGGMGRVYEARHTRLANKRF
                     AIKMLHQELARQAEVVTRFQREAEAASVLTHPNVVGVFDVNTAPDGRPYIVAELLEGE
                     QFGEHLDRRSKLSVAEAVPVVRAVCTALSLAHENGIIHRDVKPENVFLLSGAGGVKVL
                     "
     gene            complement(787..2139)
                     /locus_tag="VFQ35_21930"
     CDS             complement(787..2139)
                     /locus_tag="VFQ35_21930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET9933386.1"
                     /translation="MAAGSPVTVAQSPALAQVGNTSIADIAERVLPSVVNISLTKVSK
                     GQSGPVPFPFFFGPQDMGERREQGMGSGVIVSSDGYILTNNHVVSDASEIKVTTYDKH
                     EYDATVVGTDPKSDIAVIKLKGTVAGLKAVEFGDSSKLRLGDVVLAVGNPFGVGQTVT
                     MGIVSAKGRADLGIVDYEDFIQTDAAINPGNSGGALVNMQGQLVGVNTAILSRSGGYQ
                     GIGFAIPTNMAVPIMEALKTSGKVVRGWLGVGIQDMDSELAAGLKLPVSQGVLISDVK
                     PGAPAARAGIKRGDVVVKIDGRAVTSSGSFRNQVAALGANHSTALEVYRDGKPQTVNV
                     QLGEMPSADAAPGSHSGGPSGTGNSLDGLTLEELTPENRRALEISPEVQKGAVVTRVD
                     PRSAVARAGIRPGDVLLEVNRVPVDSLQKFQDLYSKTKGNVVLLINRHGTTVFVVVRR
                     "
     gene            complement(2382..3449)
                     /locus_tag="VFQ35_21935"
     CDS             complement(2382..3449)
                     /locus_tag="VFQ35_21935"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9933387.1"
                     /translation="MMSGSSSETPVRVLVVDDSVFNRRSISDILTTGPSIEVVGKAAD
                     GEEALRLVSALKPDAITLDLEMPRMDGFTFLRILMTKMPTPVIVVSSYSQKENVFKAL
                     ELGALDFVAKPDRYSDPDLAAIRDELVRKVLLARSVRRLASIAQRRIPDAPPARHVDA
                     TPASSLVQPKYVVAIASSTGGPSALMEIFARFPENYPHGVVIAQHMPDKFTRTFAERL
                     DRRSNLSTAEAQDRDALGAGSAFVCPGRQCMELENAPRGLRVRVSGPAEQDRYVPSAD
                     RLLTSVAQAVGRRAVGVILTGMGDDGVQGARAIMAAGGIVVAESETTAVVFGMPGSAV
                     RAGATSMVLPLPEIGEYLARL"
     gene            complement(3446..4327)
                     /locus_tag="VFQ35_21940"
     CDS             complement(3446..4327)
                     /locus_tag="VFQ35_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9933388.1"
                     /translation="MKRWDNDSRLGISPDEFRLLRDLVYESSGLRFEEDAMLLFDRRL
                     AERVGALGLSGFNAYYKYLRFNPLGGAELEEVLERLTTKETYFFRQEYQLRAFRDELL
                     PRLSYSNREQKRLSVWSAGCATGEEAYTLAMLIHQSSLFQGWDVRVIGSDISKRGVAH
                     ARRGIYRAASFRVTSPEMRSRYFREGPEGYQVIDEVKNLCHFGQLNLLDQARAGFVGR
                     VDVVFCRNVLIYFDVKSRRRVIDILYERLLPGGYLLLGHSESLLNVTTAFELVHLRED
                     LVYRKPTTTERWDRGRV"
     gene            complement(4324..>6450)
                     /locus_tag="VFQ35_21945"
     CDS             complement(4324..>6450)
                     /locus_tag="VFQ35_21945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9933389.1"
                     /translation="SGSLHAGGALTMDSEHSVLLSSVDPEERRRATAELADFDSSTVA
                     EQVTIALADEDWRVRKEAVSVALALAPSPVLLERLVDAFLPSDNVGLRNAAVEALGGY
                     GEAAVQALAARMQQFDADGRKLAVEALGRSGRASALPVLAERLLDVDLNVRAAAAEAT
                     AAIGSAGILEAVPLLENCLLSDDPLVALAALDGLNALGAVLPWRVVSACLARPSLRRA
                     ALLAAGRSADPRAVPVLLDALGSAHGTLLADVVTALRDFVRDPALSPRVREAALGLSE
                     KAKAELLRLAADEETRESARRAALVVVGALGFDGAAECAVAALGDDRFLAEAHEALEL
                     LSERALKALVRATRSGDMTARGSSLGLLARLETPSLPAAAIEAARDALDDVFPEVQSE
                     ALAVLSRYGDADCLSAVGKSLAPEAPTISAKAAENALRQLSMRCPSEARKLVRGARPD
                     APDARAACVIIGALANDSREADADLGFLTAALSNSHGSVRSAAVEALAAVGGARAVSA
                     VAFALADEEQEVRRVAIAALGKIRAEDGSAPGVAHLTELVQRGNDAESIAAAARALGE
                     AGDHHAVSVLRPLIRSQQPFVAVSAVEALAELAGPRRVECLLDGLSHADAEVVKATML
                     ALCDAPDPRVVAHLGACLDHDAWDVRRLAADLLGRNPGETALSFLRARLASEDTPPVQ
                     AAITRAIERAAGQRRSAPPGGSLRPR"
BASE COUNT         1102 a         2237 c         2090 g         1021 t
ORIGIN      
        1 cgagcacctt cacgccccct gccccactga gcagaaagac gttctcgggc ttcacgtcgc
       61 gatggatgat gccgttttcg tgcgcgagcg aaagagcggt gcacaccgcg cgcactaccg
      121 ggaccgcctc ggcgacgctc agcttgcttc gccggtcgag gtgctcgccg aactgctcgc
      181 cctcgagcag ctccgccacg atgtaaggcc gaccgtcggg cgcggtgttc acgtcgaaca
      241 cgccgaccac gttcgggtgc gtcagcacgc tcgccgcctc tgcttcgcgc tgaaaacgag
      301 tgacgacctc cgcctggcga gccagctcct ggtggagcat cttgatggcg aagcgcttgt
      361 tcgcgagacg cgtgtggcga gcctcgtaga cgcgccccat ccccccttcg ccgatcaccc
      421 gtacgatctc gtacgtgccg gagagcagct gaccgaccat cgcgtcttcg tcgctcaccg
      481 cgtcttcgag cggcaccgcg tcacgcgggc aaaccttgaa atcggcaggg aagcgccctc
      541 cgcagcgagg gcactgccgc gagttcaaca gcgtcgcggg gccagacggc gactcaccag
      601 aaagcgagcc cggtcgttcg ggcgctaacg gagtcgattg acgaagctcg gacatctctg
      661 cccagcatcg tagcgccccg cgcgctcgcc gcataccggc accgacgggg cgaacgaacg
      721 aatctcgggc ggcaaccgct cttcagcgcg gccgccgggc tctgccgtgc cgaccgccgc
      781 ttccactcag cgacggacta ctacaaacac cgtcgtacca tgacgattga tcagcaaaac
      841 gacgttccct ttcgtcttgc tgtaaaggtc ctggaacttc tgcaagctgt cgacgggtac
      901 gcgattgacc tcgagcagca cgtcgccggg gcgaatgcct gcccgcgcca ccgcgcttcg
      961 cggatcgacc cgtgtgacga ccgcgccttt ctggacttcc ggtgaaatct cgagggcgcg
     1021 gcggttctcg ggggtcaatt cttcgagcgt gagcccatcg agtgagttgc ctgtccccga
     1081 gggaccgccc gagtgcgagc cgggggccgc atctgcgctc ggcatctcgc cgagctgcac
     1141 gtttacggtc tggggtttac cgtcgcgata cacctcgagc gcggtggagt gattggcgcc
     1201 gagcgccgcg acctgattgc ggaacgagcc cgacgaggtc acggcgcgtc cgtcgatttt
     1261 caccacgacg tcgccgcgct tgatcccagc acgcgctgcg ggcgcacctg gcttcacgtc
     1321 cgagatgagg acgccctgac tgaccggaag cttcagccct gccgcgagct cactgtccat
     1381 gtcttggatg ccgacgccga gccaaccgcg caccaccttt ccgctggtct tcaatgcctc
     1441 catgatgggc acggccatgt tggtggggat ggcgaagccg atgccctggt agccaccgct
     1501 gcgcgacaag atcgccgtgt tcacgccgac gagctgccct tgcatgttca cgagcgcacc
     1561 gcccgagttt ccggggttga tggcggcgtc ggtttgaatg aagtcctcgt aatccacgat
     1621 gccgaggtcg gcgcggccct tggccgacac gatgcccatc gtcaccgttt gcccgacgcc
     1681 gaacggattg ccgaccgcga gaaccacgtc gcccagccgg agcttcgacg aatcaccgaa
     1741 ctcgacggcc ttgaggcccg cgacggttcc cttcaacttg attacggcga tgtccgattt
     1801 cgggtcggtg ccgaccacgg tcgcgtcgta ttcgtgcttg tcgtaagtcg tgaccttgat
     1861 ttcgctcgcg tccgacacga cgtggttgtt cgtgaggatg tagccgtcgg aagaaacgat
     1921 gacgcccgaa cccatgccct gttctcggcg ttcgcccatg tcctggggcc cgaaaaagaa
     1981 cggaaacggg acgggcccgc tctggccttt gctcaccttc gtgagcgaga tgttcacgac
     2041 cgagggcaga acacgctcgg cgatgtctgc gatcgacgta ttgccgactt gagcgagggc
     2101 cggcgactgc gcgaccgtga cgggcgagcc ggctgccacg gttgctcgcg caggatccgg
     2161 ctttgcttcg gcgcgcgcgg aattcgcggc gcatccggcc gccgaggcga gcgccaacaa
     2221 tgcgactgcg cccaaatgac ttcgacgacg cgaccctaga ttacgcgaga ccatttcgtg
     2281 agttcctcgt ttgctgcgag gtgaaaccta cgacggtgcc gaagcattcc cgggaagggg
     2341 caccaccgcg actaggaccg cctcacggcg cggccgtcga ctcacaaacg cgcgagatac
     2401 tcgccgattt ccggcaacgg caagaccatg ctcgtcgctc cggctcggac cgccgaccca
     2461 ggcatcccaa agaccacggc ggtggtttcg gattcggcca cgacgatgcc tccagccgcc
     2521 atgatggcgc gggccccctg cacgccgtcg tcgcccatgc cggtgaggat cacgccgacc
     2581 gcgcggcgcc ccaccgcttg cgcgaccgag gtcagcaagc gatccgcgct cggcacgtac
     2641 cggtcttgct cggcaggtcc ggacacgcgc acgcgcagac cacgcggcgc gttttcgagc
     2701 tccatgcatt gacggccagg gcacacgaag gcgctgcccg cgccgagggc gtctcggtcc
     2761 tgagcctcgg cggtgctgag attgcttctt cgatcgagcc gctcggcgaa ggtgcgcgtg
     2821 aacttgtccg gcatgtgctg cgcgatgacg acgccgtgcg ggtagttctc cgggaagcgc
     2881 gcgaagatct ccatcaacgc gctcggcccg cccgtggacg aggcgatcgc gacgacgtac
     2941 ttcggctgca ccaacgacga cgcgggcgtc gcgtccacgt gccgcgccgg gggagcatcg
     3001 gggatgcgcc tttgagcgat gctggcgagc cgccgcacgc tgcgagcgag caacaccttg
     3061 cgtacgagct catcgcgaat cgccgccaaa tccggatcgc tatatcgatc gggcttggca
     3121 acgaagtcga gcgcgccgag ctccagcgcc ttgaacacgt tctctttctg gctgtagctc
     3181 gagacgacga tcaccggagt cggcatcttc gtcatcaaga tgcgcaagaa cgtgaagccg
     3241 tccatgcgag gcatctcgag gtcgagcgtg atcgcgtcgg gcttcagcgc gctcacgagg
     3301 cgcagcgctt cctcaccgtc cgcggcctta ccgaccacct cgatgctggg tccggtcgtc
     3361 agaatgtccg aaatgcttcg acgattgaag accgagtcgt cgacgaccag gactcgaacc
     3421 ggggtttcgg agctagatcc gctcatcata cccgacctct gtcccaccgt tcagtggtgg
     3481 ttggctttct gtacacgaga tcctcacgca aatgcactag ctcgaatgcg gtcgtcacgt
     3541 tcaaaagaga ttccgagtgc ccgagcagca ggtagccgcc cggcaaaaga cgttcgtaga
     3601 gaatatcgat cacgcgccgc cgcgatttca cgtcgaaata gatgagcacg ttgcggcaga
     3661 agacgacgtc cacgcgaccc acgaagccgg cccgagcctg atcgagcaga ttcaactgac
     3721 caaaatggca caggtttttg acttcgtcga tcacctggta gccctcgggt ccttcgcgga
     3781 aatagcgcga gcgcatctcg ggtgacgtaa cccgaaagga agcggctcga taaatgcctc
     3841 gccgagcgtg cgcgacaccg cgtttcgaaa tatcgctgcc gatgacccgc acgtcccacc
     3901 cctggaacag cgagctttgg tggatgagca tcgccagcgt gtacgcttcc tcgccggtgg
     3961 cgcagcccgc gctccacacc gagagccgct tctgttcgcg attggagtac gagagccgcg
     4021 gcaaaagctc gtcgcgaaaa gcgcgcagct ggtactcctg gcggaagaag taggtctctt
     4081 tcgtcgtgag ccgctcgagg acctcttcga gctccgcgcc gcccagcggg ttgaagcgca
     4141 agtacttgta gtacgcgttg aatccggaga gccccagcgc gccaacccgc tccgcgaggc
     4201 gccgatcgaa gaggagcatc gcgtcctcct cgaagcgcag cccggagctt tcgtaaacga
     4261 ggtcgcgcag taggcggaac tcgtcgggcg aaatcccgag cctcgagtcg ttgtcccagc
     4321 gcttcatctg ggtctcagac tccccccggg cggtgcgctg cgccgctggc cagccgcgcg
     4381 ctcgatggcg cgcgtgatgg cggcttgcac cgggggggtg tcctcggacg ccaaacgcgc
     4441 tcgcaagaag ctcagcgcgg tttcccccgg attgcgaccc aatagatctg cggccaggcg
     4501 acgcacgtcc cacgcgtcgt ggtcgaggca cgcgccgaga tgcgccacga cgcgagggtc
     4561 cggtgcgtcg cataacgcga gcatcgtcgc cttaacgacc tcggcgtcgg cgtgagaaag
     4621 gccgtccagc aaacattcga cgcgccgcgg cccggcgagc tccgccagcg cttcgaccgc
     4681 cgacaccgcg acgaacggct gctgtgagcg gattaacgga cgcaagaccg acaccgcgtg
     4741 gtggtcgccg gcctcaccca gcgcgcgcgc cgccgccgcg atcgactcgg cgtcgttgcc
     4801 gcgctggacg agctcggtga ggtgagcaac gccaggagca ctgccgtctt cagcgcggat
     4861 cttcccgagc gccgcgatcg cgacccgacg cacctcttgc tcttcgtctg cgagcgcgaa
     4921 tgcgacggcc gaaacggcac gcgcgcctcc caccgccgcg agcgcttcca ccgccgccga
     4981 gcgcacgctg ccgtgcgagt tcgagagcgc cgccgtcaga aagccgagat ctgcatcggc
     5041 ctcgcgcgag tcgttcgcga gcgccccgat gatgacgcac gcagcgcgcg cgtccggcgc
     5101 gtcgggccgc gccccgcgga cgagcttgcg cgcttcgctc gggcaccgca tggagagctg
     5161 ccgcagcgcg ttctcggcgg ccttggccga tatggtcgga gcttccggcg caaggctctt
     5221 gccgaccgcc gacaagcaat cggcgtcgcc gtagcgcgag agcacggcga gcgcctcgct
     5281 ctgcacctcg gggaacacat cgtcgagcgc gtcgcgcgcc gcttcgatgg cggccgccgg
     5341 cagtgacggc gtttcgagtc gggccaagag cccgaggctg ctcccgcgcg cggtcatgtc
     5401 gcccgaacgc gtcgcgcgaa cgagcgcctt cagcgcgcgc tccgacagca actcgagcgc
     5461 ctcgtgcgcc tcggccaaaa agcgatcgtc gccgagcgcg gcgacggcgc actcggcggc
     5521 cccgtcgaaa ccaagagctc ccaccaccac cagcgccgca cgccgcgcgc tctcgcgcgt
     5581 ctcttcgtcc gcggccaagc gcaagagctc ggccttcgcc ttttcgctga ggccgagcgc
     5641 ggcctcgcgc acacggggag aaagcgccgg atcgcgcacg aagtcgcgta gcgccgtgac
     5701 gacgtcggcg agcaacgtgc cgtgagcgga accgagcgcg tcgagcaaga ctgggacggc
     5761 gcgagggtca gcgctgcggc ccgccgcgag cagcgccgcg cgccgcaagc tcgggcgtgc
     5821 aaggcacgcg gacacgaccc gccacggcag caccgcgccg agagcgttca gcccgtccag
     5881 agccgcgagc gcgaccagcg gatcgtccga cagcaagcag ttctcgagca ggggcaccgc
     5941 ctcgagaatg cccgcactgc cgatcgccgc ggtcgcttcg gcggcagccg cgcgcacgtt
     6001 cagatcgacg tcgaggaggc gctctgcgag caccgggagc gcgctcgcgc ggccggagcg
     6061 accgagcgcc tcgaccgcaa gcttgcggcc gtcggcgtcg aattgctgca tgcgagccgc
     6121 gagcgcctgc accgcggctt cgccgtaacc cccgagcgct tcgacggcgg cgtttcgaag
     6181 cccgacgttg tccgacggca agaacgcatc gacgagccgt tcgagcaaga ccgggctcgg
     6241 cgcgagcgcg agtgcgaccg acacggcttc tttgcgcacg cgccagtcct cgtcggcgag
     6301 cgcgatcgtc acttgctccg cgaccgtgct ggagtcgaag tcggcgagct cggcggtcgc
     6361 gcgccggcgc tcttcgggat ccacggacga gagcaagacg ctgtgctcgg agtccatcgt
     6421 cagcgcgcct cccgcatgta agctgccgga
//
LOCUS       DASTKS010001038         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1952250, whole genome shotgun sequence.
ACCESSION   DASTKS010001038 DASTKS010000000
VERSION     DASTKS010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952250"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFQ35_21950"
     CDS             <1..539
                     /locus_tag="VFQ35_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933390.1"
                     /translation="LHRLRGDDSGEFELLLSEIRAVISRRETAPAVLETLRPPAYGPV
                     WRHTLAARVLNELALRPECVVPRIEFASYVLDSRALAQLFVTIQEARLLHVDAQRAAF
                     GAIDRSTAPGIVEELLRSERPEKLRRLALHALVARAHARGWSREQRALLASYRADPSM
                     LVSSAASWVFPPPLPAKR"
     gene            708..2543
                     /locus_tag="VFQ35_21955"
     CDS             708..2543
                     /locus_tag="VFQ35_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933391.1"
                     /translation="MRTFAWHEFMRREQRRLVLAASVHVAATIALGTLFSRVGSIVGI
                     CGLVAVVSSAFLFGLKGALVAAVAQLALNTTVMQLIIVPAVPIDGPSAVGILFFFVAG
                     AVVGNQRDLSRKLRRELALNETLRVREKETLAAIPDAMIRIDSAGKCHLQPDPTSTKL
                     VAALESVVRQPLPPEKIEDLDAAIAKARSSGEAQLLSLELPGHTFYDLRCLPAADASL
                     LLVIRDMTELRRLLRRLTSAENLASLGTLAAGLAHEINNPLTYIITSLSSISRTSDAC
                     QSSVLAEVDRALDGCWRIRDLVRNILETTTSRRDEIDAVLVPEALEAALALVNPEVRH
                     RATVKLELDESLYVEAHRTRLMQVVVNLLTNASQAFDDHRASTNQIVVRAFCDHEWVV
                     VEVEDNGRGMDEATRQRAIEPFFTTQPVGQGSGLGLFLCSSIIESLGGSLQIESALGR
                     GTKVTFKLRATEAAPSRSGIATHSTSRAPSLAPAPRLNILVIDDEPDIRRSLRRLLGV
                     KHNIALATNGAEALRRFVQGERFDVILCDLLMPELTGIELFSELQQRFPEQSQRVVFL
                     TGGATSEAARSFIEENRSRVLNKPFGPADIESKVSRFARVSPHHS"
BASE COUNT          445 a          921 c          834 g          491 t
ORIGIN      
        1 cgctgcaccg cctgcggggc gatgacagcg gcgagttcga gctccttctc tccgaaatcc
       61 gcgccgtgat cagccgccgc gaaaccgcgc cggcagtcct cgagacgctg cgtccccccg
      121 cctacggtcc cgtgtggcgt cacactctag ccgccagagt cctaaacgag ctcgcgctgc
      181 gcccggagtg cgtcgtcccg cgcatcgaat tcgcaagcta cgtcctcgac tcccgagcgc
      241 tcgcgcagct tttcgtcacg atacaggaag ctaggctcct tcacgtcgac gcccagcgcg
      301 ccgcgttcgg cgcgatcgat cgctccaccg cgccgggcat cgtcgaggag ctcttgcgct
      361 ccgaaaggcc cgaaaagctg cgccgcctcg ccctgcacgc gctcgttgct cgcgcacacg
      421 cgcgcggctg gagccgtgaa caacgcgcgc tcctcgcgag ctatcgcgcc gatccgtcga
      481 tgctcgtctc gagcgcggcg agctgggtat ttcctccgcc gttgccagcc aagcgttgag
      541 cgcaggcctc cgcctcgccg cgctcgggcg ctgcagaggg ccgtgtgagg ccgcgcttac
      601 acccgagcat atggttcgtc tcgcgcagtc tcatatcgtg acgatcctcg atatatttcg
      661 cgcgggacct gatgccgggt cgaaactcgc cgtgttgggt agatgacgtg aggaccttcg
      721 cctggcacga gttcatgcgg cgcgagcaac ggcggctcgt gctggcggcg tcggtgcatg
      781 tggcagctac catagctctc gggacgctgt tctcgcgcgt cggctccatc gtcgggattt
      841 gtggtctcgt cgcggtcgtg tcgagcgcgt ttttgttcgg cctcaaaggg gcactcgttg
      901 cggcggtcgc gcagctcgcg ctgaacacga ctgtaatgca actgataatc gttcccgcgg
      961 tgccgatcga cgggccctcc gcggtgggga ttctcttctt cttcgtcgcc ggagccgtgg
     1021 tcggcaatca gcgtgacctg tctcgaaagc tgcgacgcga gctcgccttg aacgagactc
     1081 tgcgcgtgcg cgagaaagaa acccttgccg caattcccga cgcgatgatc cgcatcgatt
     1141 cggcagggaa gtgccacctg cagcccgacc cgacttcgac gaagctcgtg gccgcgctcg
     1201 aaagcgtcgt tcggcagcct ttgccgcccg agaaaatcga ggacctcgat gcagccatcg
     1261 ccaaggcaag gtcgtccggc gaggctcagc tgttgagcct ggaattgcca ggccacacct
     1321 tctacgatct gcgctgccta cccgctgccg acgcttcgtt gctgttggtc attcgtgaca
     1381 tgaccgagct ccgacgcctg ctgcgccgac tcacatcagc cgagaacctc gcctccctcg
     1441 gtacgttggc ggctggcctc gcccacgaga tcaacaaccc gctcacgtac atcatcacga
     1501 gcctgtcttc catttcgagg acctccgacg cttgtcagtc cagcgtgctg gcggaggtcg
     1561 accgagctct cgacggttgc tggcggattc gagatttggt ccgcaatatc ctcgagacca
     1621 cgaccagccg aagagacgag attgacgcgg tgttggttcc agaggcgctc gaggcggcgc
     1681 tcgcgctcgt gaaccccgag gtgcgccacc gcgcgacggt gaagctcgag ctcgacgagt
     1741 cgctctacgt cgaagcgcat cgaacacggc tcatgcaggt cgtcgtgaac ctgctcacga
     1801 acgcgagcca agccttcgac gatcatcgcg cgagcaccaa ccaaatcgta gtgcgagcct
     1861 tctgcgacca cgagtgggtg gtcgtggagg tcgaagacaa cggccgcggg atggatgaag
     1921 ccactcgcca gcgcgcgatc gagcccttct tcacgacgca gccagtcggg cagggttccg
     1981 gcctcggact cttcttgtgt agttcgatca tcgagtcgct cggaggcagc ctgcaaatcg
     2041 agagcgcgct tggacgcgga accaaggtga cgttcaagct gcgcgctacc gaagcggctc
     2101 cgtcgcggag cggcatcgcg acgcatagca cttctcgggc gccgtcgctc gcgcccgccc
     2161 cgcgactgaa cattttggta atcgacgatg aacccgatat ccgtcgctcg ctgcgccgac
     2221 tgctcggcgt gaagcacaac atcgctctcg cgacgaacgg cgccgaagcg cttcggcgtt
     2281 tcgtccaagg cgagcgcttc gacgtcatcc tgtgtgacct cctcatgccc gagctgaccg
     2341 ggatcgagct cttcagcgag ctccagcagc gcttcccgga gcaatctcag cgcgtcgtgt
     2401 tcctcaccgg aggggcaacg tcggaagccg cgcgctcgtt catcgaagag aaccgctcgc
     2461 gcgtgctgaa caagccgttc gggcccgccg atatcgaatc gaaggtttct cggttcgcga
     2521 gggtctcccc gcaccactcc tgaatcctgg accgaacccg ggccaagaga cgcgagagct
     2581 cccctgcccc gtccgcgcac gccgcattcg ccgactccgc gaggccgggc gctgcgttcg
     2641 ccgggcgttc ctggaccgca gggctcgagc gtgcgtgtct tcgaagcgac g
//
LOCUS       DASTKS010001039         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_610650, whole genome shotgun sequence.
ACCESSION   DASTKS010001039 DASTKS010000000
VERSION     DASTKS010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610650"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1289)
                     /locus_tag="VFQ35_21960"
     CDS             complement(30..1289)
                     /locus_tag="VFQ35_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933392.1"
                     /translation="MKRFAFALLYVSLAACEHRRAAPAAANSSPHVAALSASAPASES
                     SARPAPTVLSVDQHMTKIFAPAHQLQPYRGPRVKRIVDRCAVLEDESVRCFDGFEQLA
                     ARVEKVHLGSAGLFSEADEWKSGCAADTQGLVSCWGNVRAHTLGDKSRCHVVPLPPNP
                     EGEDRKCPEIDGLYYCGIRARVCDEPTRLARLGPVTQLAGAEHNCALNAKGELWCWGD
                     NARNEGPGRDRCRIPRGTNGQLGSEYCFDPVRVLGLPPLASFSLSAARTCAVTREGKL
                     FCWGAFESVQYDAPTEQKDWPRLTEAVLTDGFLCGRTEAGEVHCQREGQQKPEVVPGL
                     GRVTKLTGDALTCALNERGEVWCWMRSYPPFDKPHQVPAITSATQISARAGFACAILK
                     DGAAYCWGSWTYRGAFESTPPLPLFDP"
     gene            complement(1286..3019)
                     /locus_tag="VFQ35_21965"
     CDS             complement(1286..3019)
                     /locus_tag="VFQ35_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933393.1"
                     /translation="MSRLRIELVGARNHKPLITVFKRVSDKNEQKSQSVDPVLETDVG
                     SDDLRIVVEFSIPKEILSAQKKPQAMAAFAQNLNFVPGSGVQPASHQPAGSNAGPKLH
                     PRFLPVQGTGASAGSGALIHYRVDCRFLPMTELLRSLDNKALDTLDSTVPDPLRPSGA
                     EVRVYECTVGTPRFWVVAFAPGAAPPAVTNLHPVVFFMPKSPTHSNLTDLSLFSVRRY
                     LLSAPKNHPFFVRCFQPNDPLTDADWPSTVTNAALLEQVVQSGKPILLALPVPSGSSI
                     VPSGGNIRELMTALRRAVHGDDLGKAEELQPAFRVGVSGFSHGADTAADLLSKQPGSV
                     DEFYWMDPGTDGMSKSSAVEAWVQGNATRRLRLIGTTINAFMHALANRLAALGARVNA
                     MPPPDFRKSDIYRAAVGVPIHTTSIPFPETFQDAANPAAPPPGSLSDATGIFVVSDSR
                     SGTSPALTLQVRDLTNKPIPSDGVQAAPFTVEEIAGKARFGMLNVDRKSPPPGVTPFG
                     ERLHGPKNRTLRDFIARFAVGEILDNNNVGDGSIHQWAAVGGLGGGGSSPRQGFTGFF
                     QSALERGDFPK"
     gene            complement(3016..>4749)
                     /locus_tag="VFQ35_21970"
     CDS             complement(3016..>4749)
                     /locus_tag="VFQ35_21970"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9933394.1"
                     /translation="TESLTDKEREGEAPPLRALPPPEPETSFIAVNLIDQKGKPVIGR
                     RWIIELPDGTKHEGVTDAEGWARVKGFTQDGLAKVSFPQFDELDHETRVSAQRIIEPI
                     HGDEEAPAPDEQAEPENVAKPKAPSAAPKPDEQSALPDLAELASAAELVEQLPGQEAL
                     PIGKIFMELWDAFGEERHANRSYTIAGPVPLTGTTDASGRLEHATVPAGDYVLRLTLD
                     DGEFVSPALVQETAAGAPHVRFIGPGKSAVVARLIGMFFDTSKSFLLPTAIPAIRDVR
                     RLYDQHPGSKLLIVGHTDTAGKPTYNDPLSLERADAIAAFLQDQAELWLAWYGANKPT
                     EKRWGDAEDTSMVGALDDAASRPPSEAPMTWFRRTRNLTSASLRDVRLALVREYMSAD
                     GTSLPKDIEVTTHGCGENFPAVTTGDEVDLQDNRRVELFFFDDAIKPPPPGKNSPRGS
                     TAYPKWLASVRETFDLRIGAARTTHLVVSLYVGERFPDRSGSVIVSTAAGNEAARFAI
                     KDLPFGAPGHVFVELDTSTLPDPVAIRRVTEFEDITLIGPCSLKQLCSALDSGDFESA
                     GAIGFPKQVSA"
BASE COUNT          769 a         1473 c         1635 g          872 t
ORIGIN      
        1 cttcgtcatg tcgaagaacg cacgaggcct tacggatcga acaagggaag cgggggcgtg
       61 ctctcgaacg cgccgcggta ggtccaactg ccccagcagt aggcggctcc atccttcagg
      121 atcgcgcagg cgaagccggc acgagccgag atttgcgtcg cagacgtgat ggcagggact
      181 tggtggggtt tatcgaacgg agggtacgac cgcatccagc accaaacctc gccgcgctcg
      241 ttcagcgcgc aagtgagggc gtcgcccgtg agcttcgtta ctctgccgag cccgggcacg
      301 acctcgggtt tttgctgccc ttcgcgctgg caatgcactt cgcccgcctc ggtgcgaccg
      361 cacaggaacc cgtcggtcaa gacggcttcc gtgagccgcg gccaatcctt ttgctcggtt
      421 ggagcgtcgt attggacgga ctcgaatgcg ccccagcaga agagcttgcc ctcgcgcgtg
      481 acggcgcacg tgcgtgcggc cgaaagcgag aacgacgcga gcggcggcaa cccaagcacg
      541 cgaaccgggt cgaagcagta ctccgagccg agctgcccgt tggtgccgcg cggaattcgg
      601 caacgatcgc gaccgggacc ttcgtttcgc gcgttgtcgc cccaacacca gagctcgcct
      661 ttggcgttca acgcgcagtt gtgctcggcg ccggccagct gggttacggg tccgagccgc
      721 gctagccgcg tgggttcgtc acacacgcgc gcgcggatgc cgcagtagta caagccgtcg
      781 atttcagggc acttacgatc ttcgccttcg gggttcggcg gtagaggaac tacgtggcag
      841 cggctcttgt cgccgagcgt gtgcgctcgc acatttcccc agcacgagac gagcccttgg
      901 gtgtccgcgg cgcaacccga cttccattcg tccgcttcgg agaacagccc cgccgacccg
      961 aggtgcactt tctcgacccg cgccgccaac tgctcgaagc cgtcgaagca ccgcacgctt
     1021 tcgtcttcga gaacagcgca gcgatcgacg attcgcttca cgcgcgggcc gcgatatggc
     1081 tgcagttgat gagccggcgc gaagatcttg gtcatgtgct gatccaccga gaggacggtc
     1141 ggcgctggtc gagcgctgct ctccgaagcc ggagcgctcg ccgagagtgc ggccacgtga
     1201 gggctcgagt tcgccgccgc gggtgcggca cgccgatgtt cgcaggccgc gagcgagaca
     1261 tacaggagcg cgaaggcgaa ccttttcact tcggaaagtc tcctcgttcc aacgccgact
     1321 ggaagaagcc agtgaacccc tgccgtggcg atgagcctcc gcctccgagc ccgccgacgg
     1381 ccgcccactg atggatgctg ccgtcgccga cgttgttgtt gtcgaggatt tcgccgacgg
     1441 cgaagcgcgc gatgaagtcg cgcagagttc tgttcttcgg tccgtgcaag cgctcgccga
     1501 atggcgtgac gccgggtggc gggctcttgc gatcgacgtt cagcatcccg aagcgcgctt
     1561 tgcctgcgat ttcctcgacg gtgaagggcg cggcctgaac gccgtcgctc gggatcggct
     1621 tgttggtgag gtcgcgcact tggagagtga gcgccgggct cgtcccgctg cggctgtcgc
     1681 tcacgacgaa tatgccggtc gcgtcggaga ggctgcccgg gggaggagcg gcggggtttg
     1741 ccgcatcctg aaaagtctcc gggaagggga tgctcgtggt gtgaatcgga acgccgacgg
     1801 cggcccggta gatgtcgctc ttgcgaaaat cgggcggggg cattgcgttt acgcgtgcgc
     1861 cgagcgcggc gagccggttc gcgagcgcgt gcatgaacgc attgatggtc gttccgatca
     1921 agcggagccg ccgcgtagca ttgccttgca cccaggcctc gacggcgctg ctcttcgaca
     1981 tgccgtcggt accagggtcc atccagtaga attcgtcgac ggacccgggt tgcttcgaca
     2041 agagatcggc ggccgtgtcg gcgccgtggc tgaagccgct cacgccaacg cggaacgcgg
     2101 gctgaagctc ttcggctttg ccgaggtcgt cgccatggac ggcgcgtcgc agcgccgtca
     2161 tgagctcgcg gatgtttccg ccgctcggca cgatcgagct gccgctcggg acgggcaacg
     2221 cgagcaagat tggcttgccg ctttgcacca cctgctcgag cagggccgcg ttcgtgaccg
     2281 tcgatggcca atcggcgtcg gtcaaagggt cgttcggctg gaagcagcga acgaagaagg
     2341 gatggttctt cggagccgag agcagatagc ggcgcacgct gaaaaggctg agatcagtga
     2401 gattcgagtg cgtgggcgac ttcggcatga agaagaccac cgggtgcagg ttcgtcacgg
     2461 ctggtggggc cgcgccgggc gcgaacgcga cgacccagaa gcgcggcgtt ccgacggtgc
     2521 actcgtaaac gcggacctcc gcgccgctcg ggcgcaacgg atctggcact gtcgagtcca
     2581 gcgtgtcgag cgccttgttg tcgagcgatc tcaacaactc ggtcatgggc aagaagcgac
     2641 aatccactcg gtagtggatg agcgcgccgc tgccagcgct cgcccctgtg ccctgtaccg
     2701 gcaagaagcg cggatgaagc ttggggccgg cgttcgagcc ggcaggttga tgggacgcag
     2761 gttgaactcc gctgccgggc acgaagttca aattctgtgc gaacgcggcc atcgcctgag
     2821 gcttcttttg ggcggacagg atctccttgg ggatcgagaa ctcgacgacg atgcggaggt
     2881 cgtcgctgcc gacgtcggtc tcgagcacgg ggtcgaccga ctgcgacttt tgctcgttct
     2941 tgtcgctgac gcgtttgaag accgtgatga gcggcttgtg gttgcgtgcg ccgacgagct
     3001 cgattcgaag gcggctcatg cgctcacctg cttcggaaag ccgattgcgc cggccgactc
     3061 gaagtcgccc gaatcgagcg cgctgcacag ctgcttgagc gagcagggcc cgatgagcgt
     3121 gatgtcttcg aactcggtca ctctccggat cgcgaccgga tccggcaggg tgctcgtgtc
     3181 gagctcgacg aagacgtggc caggcgcgcc gaagggcaag tccttgattg cgaaccgggc
     3241 cgcctcgttg ccggccgcgg tcgagacgat caccgagccg ctgcgatccg gaaagcgctc
     3301 accgacgtag agcgagacga cgaggtgcgt cgtgcgtgcc gctccgatgc gaaggtcgaa
     3361 cgtttctctc acgctcgcga gccacttggg gtaggcggtg cttccgcgcg gcgagttctt
     3421 gcccggggga ggcggcttga tggcgtcgtc gaaaaagaag agctccacgc ggcggttgtc
     3481 ctgcaggtcg acctcgtctc cggtcgtcac ggccgggaaa ttctctccgc agccgtgggt
     3541 cgtgacctcg atgtctttcg gcaacgaggt gccatcggcg gacatgtatt cacgaaccag
     3601 cgcgaggcgc acgtcgcgaa gcgatgccga cgtcaaattc ctcgtgcgtc gaaaccacgt
     3661 catcggagcc tctgatgggg ggcgactggc ggcgtcgtcg agcgcgccca ccatcgaagt
     3721 gtcttccgca tctccccagc gcttttcggt cggtttattg gccccgtacc aggcaagcca
     3781 aagctccgct tggtcttgga ggaacgcggc gatggcgtct gcgcgctcga gtgaaagagg
     3841 gtcgttgtaa gtcggcttcc ctgccgtgtc ggtgtgcccg acgatgagca gcttgctacc
     3901 aggatgctga tcgtagaggc gacgcacgtc gcggatcgcc gggattgcgg tcggtagcag
     3961 aaacgatttg ctcgtgtcga agaacattcc gatcaagcgc gcgaccaccg cgctctttcc
     4021 ggggccgatg aagcgcacgt gaggcgcacc ggccgcggtc tcttgcacga gcgcgggcga
     4081 gacgaactcg ccgtcatcca acgtcaggcg cagcacgtag tcgccggccg gcacggtggc
     4141 gtgctcgagc cgaccgctcg catccgtagt ccccgtgagc ggcaccggcc ccgcgatggt
     4201 gtagctccgg ttcgcgtggc gctcttctcc gaaagcatcc cagagctcca tgaagatttt
     4261 gccaatgggg agagcttcct gcccggggag ttgttcgacg agctccgctg cgctcgcgag
     4321 ctcagcgagg tcgggcagag cgctctgctc gtcgggcttg ggggcggcgc tcggcgcctt
     4381 gggtttcgcg acgttctcgg gctccgcctg ctcgtccggc gcaggcgctt cttcgtcgcc
     4441 gtgaatgggt tcgatgatgc gctgagcgga gacgcgcgtc tcgtggtcga gctcgtcgaa
     4501 ctgcggaaag ctcactttcg cgagcccgtc ttgggtgaaa cctttgacgc gcgcccagcc
     4561 ttccgcgtcc gtgacacctt cgtgcttagt gccgtcgggc agctcgatga tccagcggcg
     4621 accgatgacg ggcttcccct tttgatcgat caggttgacg gcgatgaagc tcgtttcggg
     4681 ttcgggtggg gggagcgctc gcaacggcgg cgcttcgcct tcacgttcct tgtcggtgag
     4741 gctctcggt
//
LOCUS       DASTKS010001040         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_610831, whole genome shotgun sequence.
ACCESSION   DASTKS010001040 DASTKS010000000
VERSION     DASTKS010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610831"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFQ35_21975"
     CDS             <1..1049
                     /locus_tag="VFQ35_21975"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9933395.1"
                     /translation="SLGGSWVRLRLSNAHGQRPLVVGSTHLGLQKSGASIVAGTDRAV
                     TFGGQPSITIPVGAHVLSDPVRLEVAALSNVSVSLYVPNDTGPATWQFPSGQTSYISP
                     TGDFTLASEMPTEKTDNGRYWVSGLEVLAPAASGAVVTLGDSLTQGFGSTPDTNQSWP
                     DLLAARLNARPGRFVRGVLNQGIGANRLLHEFSGNSAASRFDRDVIAQSGVTHVVISE
                     GVVDIGLPGVTGATDEVVSAAQLIQGQRQLIERAHAKGIKVIGATLTPFGGLEFPGFD
                     TPENEAKRQALNQWIRSGGAFDGVVDFDAVVRDPSVPNRLLARYDFGDHIHLNDAGYA
                     ALANSIPLSLFAAD"
     gene            1214..2674
                     /locus_tag="VFQ35_21980"
     CDS             1214..2674
                     /locus_tag="VFQ35_21980"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9933396.1"
                     /translation="MLKAMRIGFGALAMLTVACSKSRSKAPAEVESPRAPAASASERA
                     VTAPSASPTPPTEAALTDGSVKALFERWLDAQNRGDFSAYERLYAERFTGVKRSGAYK
                     ATFDRKGWLRDRKGMFTHAMRVEASHTALSVTPRTARIAFEQTWAAGNYRDVGPKQIV
                     VVASPEGPRIAREEMLSSTVAGADVRAQVNDVKLMTDEGIILDTNPQEDWASGKSIPG
                     ARESLALRNVDEAKLPAELSAWKGRSVRALGESGPSCEAKVVGFELRAEVVPHFGSVQ
                     TWHGQTGEPALTLAQIAEEIWTMAQGGGRVLVGKLAPPCVGLWALDASRSAPLAAAPE
                     PADADLNVRALRAVRTLPDYANIQKAFNETVESPKGRWEDYSSHSARVTAFRFAGKLR
                     LVEVLMSAGNGCGDFEGTLTAVYGVRKGTAAELEYLGPGSSLQPSSAFDVDGDGSLEL
                     LYGGTHDGAGARLWRKTATRTSLDELFDVPFLDCGC"
BASE COUNT          502 a          983 c          974 g          474 t
ORIGIN      
        1 tcagcctcgg aggaagctgg gtcaggctgc ggctctcgaa cgctcacggt caacgcccgc
       61 tcgtcgtggg cagcactcac ttggggttac agaagagcgg tgcctcgatc gtggccggca
      121 cggatcgagc ggtcacgttc ggcgggcagc cgtcgatcac cattccggtc ggtgctcacg
      181 tgctcagcga tccggtgcga ctcgaggtcg cggcgctctc gaacgtgagc gtgagcctct
      241 acgtgccgaa cgacactggc cccgccactt ggcagtttcc gagcgggcaa accagctaca
      301 tttcgccaac gggcgatttc acgctcgcga gcgagatgcc gactgaaaag acggataacg
      361 gtcgctattg ggtgtcggga ctggaggtgc tcgcgcccgc tgccagcgga gccgtggtga
      421 cgctcggtga ctcgctcacc cagggtttcg ggtcgacacc ggatacgaat cagagctggc
      481 cggacttgct cgcggcgcgc ctgaacgcgc gtccggggcg cttcgtgcga ggcgtcctga
      541 accagggcat cggcgcaaac cggctccttc acgagttctc cggcaacagc gctgcgtcgc
      601 gcttcgatcg cgacgtcatc gcgcagtcgg gcgtgactca cgtggtcatc tccgaaggcg
      661 tggtcgacat cggcctgcct ggcgtcaccg gcgccacgga cgaagtcgtg agcgcggcgc
      721 agctgattca gggccaacgt cagctcatcg agcgcgccca cgctaagggc atcaaagtga
      781 tcggcgcgac gctgactccc ttcggtgggc tcgagtttcc gggcttcgac acgcccgaaa
      841 acgaggccaa gcgccaggcg ttgaaccaat ggattcgaag cggtggcgcc ttcgacggcg
      901 tcgtcgactt cgacgcggta gttcgcgatc ccagcgtccc gaaccgcctc ctcgctcgct
      961 acgatttcgg cgatcacatt cacctcaacg acgctggcta cgccgcactc gcgaactcca
     1021 ttccgctgag cctattcgcc gcggattgaa ctcgcattgg tatggggttg cccccgaccg
     1081 tccagtgctc gccccttcgg ggctgcgcgc ttcctgctcc ccccggcgtc gtgctcggcc
     1141 ttcggcctgc gcgcgccgcg ggggagcccg cgcggctggc cagccctgct gcaggttggc
     1201 tatgctcgcg ctcgtgctca aagcgatgag aattgggttc ggggcgctcg cgatgctcac
     1261 cgttgcgtgc tcgaagtcgc gctcgaaggc ccccgctgag gtcgagtctc cgcgcgctcc
     1321 ggccgccagc gcgagcgaac gcgcggtcac cgcgcccagc gcttctccga cgccgccgac
     1381 cgaggcggcc ctcaccgacg gaagcgtcaa ggcgctgttc gagcgctggc tcgacgcgca
     1441 gaaccgcggc gacttcagcg cgtacgagcg actctacgcc gagcgcttta cgggcgtgaa
     1501 acgttcaggc gcctacaaag cgaccttcga ccgcaaaggc tggctgcgcg accgcaaggg
     1561 catgttcacg catgcgatgc gcgtcgaagc gagccacacg gcgctctcgg tcacgccgcg
     1621 taccgcgcgc atcgcgttcg aacaaacctg ggcggccgga aactaccgtg acgtcggccc
     1681 caagcaaatc gtggttgtcg cgagccccga aggtcctcgc atcgcgcgcg aagagatgct
     1741 gagctcgacc gtagcgggcg ccgacgtgcg cgcccaggtc aacgacgtga agctcatgac
     1801 cgacgaaggc atcatcctcg acaccaatcc gcaggaggac tgggcgagcg gaaaatcgat
     1861 cccgggagcg cgagagagcc tcgcgctccg taacgtcgac gaagcgaagc tacctgcgga
     1921 gctcagcgcc tggaagggcc gcagcgttcg cgcgctcggc gaaagcggac caagctgcga
     1981 agcgaaggtc gttggcttcg agctccgggc cgaagtggtt cctcacttcg gaagcgtgca
     2041 gacctggcat ggccaaaccg gtgaacccgc gctcacgctc gcgcaaattg cggaggaaat
     2101 ttggacgatg gcgcagggcg gtggccgcgt cctcgtgggc aagctggcgc cgccgtgcgt
     2161 cggcctttgg gccctcgatg ccagccgcag cgccccgctc gcggccgcgc cagagccggc
     2221 ggacgcggac ctcaacgtgc gcgccctccg cgcggtccgc acgttgcccg actacgcgaa
     2281 catccaaaaa gcgttcaacg aaacggtcga gtctcccaag ggccgttggg aagactactc
     2341 gagtcactcg gctcgcgtga cggcctttcg gttcgcggga aagctcaggc tcgtcgaagt
     2401 gctgatgagc gccggcaacg gctgcggcga cttcgaaggc accctcaccg cggtctacgg
     2461 cgtgcgcaaa ggcacggcag ccgagctcga gtatctcggc ccaggaagct cgcttcagcc
     2521 aagcagtgcc ttcgacgtag acggcgacgg atcgctcgag ttgctatatg gcggtactca
     2581 cgatggtgcg ggggctcgcc tttggcgcaa aaccgcgact aggacctcgc tagacgagct
     2641 attcgacgta ccgttcctcg attgcggctg ctgacggccc accaataggt gagtctgcgc
     2701 gagccgatcc tctcgctgcg gcggagggct cgtcgcggcc ctcctctttc gtgacgagcc
     2761 gctgcgagcg aacgagacga agcctcgggt gaatactctc gcgtcacggg tgccgaacgg
     2821 cccgcttcaa ccggggttcg gcgccacgac ctgcccgagg aacaggattt gcccggtggg
     2881 ctcgtcgaag atcgcgaaca cgaagggccg atccaaggtg agggtagcct ccg
//
LOCUS       DASTKS010001041         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1728036, whole genome shotgun sequence.
ACCESSION   DASTKS010001041 DASTKS010000000
VERSION     DASTKS010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728036"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFQ35_21985"
     CDS             <1..1243
                     /locus_tag="VFQ35_21985"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9933397.1"
                     /translation="IGFALQMGGVGAMGTFGGDATLANEFTIDIGGKTFGLFGTKGFF
                     LTLANFTPAVATMFLFQMVFMDTTATIPTGAMAERWKFLSFVLFSFVISTVIYPVYAN
                     WVWGGGWLSALGANYGLGHGHVDFAGSSVVHMTGGVAALVGAKMIGPRIGKFKSDGTA
                     VPMPGHNIPMALLGTFILAFGWFGFNPGSTLAGTDTRIAVVAVNTMLASASGAFASAV
                     FVWTRFGKPDPTWLANGMLAGLVAITAPCGFVGAPEAILIGAVAGVLVVLSATFIEHK
                     LKIDDPVGASSVHGVCGAWGILSLGLFANGTYGDGLNGVPGKVTGLFFGDSKQFLAEC
                     IGIGVNCIYVGLMTAGALFVIGKLVGNRVSAQDEIEGLDIPEMGMLAYPEDPPAAMPA
                     PSEKLEPAPAGMVAAAPRVAG"
     gene            1340..2584
                     /locus_tag="VFQ35_21990"
     CDS             1340..2584
                     /locus_tag="VFQ35_21990"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET9933398.1"
                     /translation="MLPSWTLLPFPALVLAMAVLPALSPHRWEKRWFQALVVACCALP
                     ILASTLLVGETPALLHTARSYATFTATLGALYVTAGGIYAMGDLEATPATNVGFLLVG
                     SLLASAIGTTGASMLVIRPLLRTNSQREHTAHLVPFFILAVANAGGLLTPLGDPPLLV
                     GFADGVPFAWTLRLFPIWVLYVSTFALGLYVVDRRAYARESPESIRRDERERRPLEVR
                     GLLNIAFLLAIVAAAFLPAGLREASMLGIAVLSYKLTPPSVHEQNQFSFGPILEIALL
                     FAGLFLCLIPIEAALAENASALPLSRAWHLFWSAGAFSAVLDNAPTYAAFAALARGLS
                     AGQPELVAGIAPLKLAAISAGSVVMGATTYIGNGPNLMVKAIAERAGMRLPSFGRFFV
                     FALVAMLPAHLIVSVAFAWLER"
     gene            complement(2605..3198)
                     /locus_tag="VFQ35_21995"
     CDS             complement(2605..3198)
                     /locus_tag="VFQ35_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933399.1"
                     /translation="MEKYVDTVKALTLAPMLIIVACAEHSNAPGAASPTPAASNPIVV
                     TPPETPNSNEASNAGAPTAPAAAPGAPSTEPSPPPPAGTPTLPAVAVHSVGMHIGGGP
                     NDAEGKAPFERALEQHFPDFLRCYRLVTEPGKGGTFGVDLHIERAGGKPRVEQPRTAL
                     GGEPFRACMLTAFESVEFPALKKPSVVSYSLRFTVES"
     gene            complement(3220..>4032)
                     /locus_tag="VFQ35_22000"
     CDS             complement(3220..>4032)
                     /locus_tag="VFQ35_22000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9933400.1"
                     /translation="TTAHDDWLELLVEAGPLPPLLLLIAIAMAARASEPHPAAALVAL
                     AVCALGDSPFHLPAVCVLVAVIFAALPRERPLARKRLLVLLLGAGGALLLAQSFGGWF
                     ASRLTTAARSEMPEGRRKLLERAVRLAPASSEYALELGLARRDAGDLTGALQMLERSV
                     ALGGDVASFVALGNVALEAGESPRARAAFEAALARNPGSVRAHTGLGEALRHLGKLGS
                     AEEHVRVALELRPGDLDLRNRLDSLREQRLDDELDSERFGEPNVLGESRNSR"
BASE COUNT          629 a         1381 c         1301 g          721 t
ORIGIN      
        1 catcgggttc gcgctgcaga tgggcggcgt cggcgcgatg gggacgttcg gcggcgacgc
       61 aacgctggcg aatgaattca cgatcgacat cggaggcaag acctttgggc tcttcggaac
      121 caaaggcttc ttcttgaccc tggcgaactt cacgcctgcg gtcgcgacga tgtttctctt
      181 ccaaatggtg ttcatggaca ccaccgcgac catcccgact ggcgccatgg cggagcgctg
      241 gaagttcctg tcgttcgtgc tgttcagctt cgtgatctcg acggttatct acccggtgta
      301 cgcgaactgg gtgtggggcg gcggctggct ctcggcgctc ggcgccaatt acggcctcgg
      361 ccacggtcac gtcgacttcg cggggagctc cgtcgtgcac atgaccggcg gcgtggccgc
      421 actcgtaggc gccaagatga tcgggccgcg catcggtaag ttcaaatcgg acggaaccgc
      481 cgtcccgatg ccgggacaca acatcccgat ggccctgctc gggaccttca tcctcgcgtt
      541 cggctggttc ggcttcaacc ccggttcgac gctcgccgga accgacacgc gcatcgccgt
      601 cgtagccgtg aacacgatgc tcgcttcggc gtcgggcgcg ttcgcgtcgg ccgtgttcgt
      661 ctggacgcgc ttcggcaagc cagacccgac gtggctggcc aacggcatgc tcgcagggct
      721 cgtcgcgatc acggcgccgt gcggattcgt cggggcgccc gaagccatcc tcatcggcgc
      781 cgtcgctggc gtactcgtgg tgctctcggc gaccttcatc gagcacaagc tcaaaatcga
      841 cgatccagtg ggcgcctcca gcgtgcacgg cgtgtgcggc gcgtggggca tcctttcgct
      901 tggcctcttc gcgaacggca cttacggcga tggactgaac ggcgtacccg gcaaggtgac
      961 ggggctgttc ttcggtgact ccaagcagtt cctcgcggag tgcatcggca tcggcgtaaa
     1021 ctgcatctac gtcggcctca tgaccgccgg cgcgctgttc gtcatcggca agctcgtcgg
     1081 caaccgcgta tcggcccagg acgagatcga agggctcgac atccccgaaa tgggcatgct
     1141 cgcgtatcca gaagatccgc ccgcggcgat gcccgctccg tccgagaagc tcgaaccggc
     1201 tccggctggc atggtcgccg cggcgccgcg cgtcgccggc taaacgagcc cgctacccca
     1261 aaaaagttcg aaagcccgcc ctgcgtcgcg cgattgggcg ggctttcgac ggctgccgag
     1321 gggttctgat aagcatccga tgttgccgag ttggacccta ctgccgttcc ctgccctcgt
     1381 cctggccatg gccgtgctgc ccgcgctctc cccgcaccgt tgggagaaac gctggtttca
     1441 agctctcgtg gtcgcctgct gcgcactacc gatcctcgcg agcacgttgc tcgttgggga
     1501 gacccccgcg ctcctacaca cggcgagaag ttacgcgacc ttcaccgcca cgctcggcgc
     1561 cctttacgtg accgccggcg gcatctacgc gatgggagac ctcgaggcga ctcccgccac
     1621 gaacgtcggc tttctgttgg tcggttcgct gctcgccagc gcaatcggaa ccaccggcgc
     1681 cagtatgctc gtgattcgac cgttgctgcg cacgaactca caacgcgaac acacggcgca
     1741 tctcgtgccg ttcttcatct tggcggtcgc aaacgcgggc gggctcctca cgccgctcgg
     1801 tgatccgccg ctgctcgtgg ggttcgccga cggggtgccc ttcgcctgga cgttgcggct
     1861 ctttccgatc tgggtgctct atgtgtcgac cttcgcgctc ggcctctacg tcgtcgatcg
     1921 ccgcgcgtat gcgcgcgaga gccccgaatc gattcgtcgc gacgagcgcg aacgacgccc
     1981 gctcgaggtg cgagggctgc tgaacatcgc gttcctgctc gccatcgtcg cagcggcctt
     2041 cttgcccgcc ggcctgcgcg aggcttcgat gctgggcatc gccgtgctct cgtacaagct
     2101 gacgccgccc tcggttcacg aacagaacca attttcgttc ggcccgatcc tcgaaatcgc
     2161 gctgcttttc gcgggcctat tcctctgcct gattccgatc gaagcggcgc tcgccgaaaa
     2221 cgcttcggct ttgccgttga gccgcgcgtg gcatctcttc tggagcgccg gcgcgttctc
     2281 cgcggtcctc gacaacgctc cgacctacgc ggcgtttgcg gcccttgcgc gcgggctcag
     2341 cgccggtcaa cccgagctcg tggcgggcat cgctccgctc aagctcgccg ccatcagcgc
     2401 aggcagcgtg gtgatgggtg ccactacgta catcggcaac ggaccgaact tgatggtgaa
     2461 agcgatcgcc gagcgcgcgg gcatgcgcct gcctagcttc ggtcggttct tcgtcttcgc
     2521 gctcgtcgcg atgctacccg cgcacctgat cgtgagcgtt gcgttcgcgt ggctcgaacg
     2581 ttgaagcgaa ggcccggctc ggactcagga ttcgaccgtg aagcgcagcg agtaactcac
     2641 gaccgacggc ttcttcaaag cggggaactc gacgctctcg aaggcggtca gcatgcacgc
     2701 acggaacggt tccccgccga gcgccgtgcg cggctgctcc acgcgtggct tgcctccagc
     2761 gcgctcgatg tgtaggtcga cgccgaacgt gccgcctttg cccggctccg tgacgagccg
     2821 atagcaccga agaaagtccg ggaaatgctg ctcgagcgcg cgctcgaagg gagccttccc
     2881 ttccgcgtcg ttcggcccgc caccaatgtg catccccacg ctatgcaccg ccacggctgg
     2941 caaggtcggc gtccccgcgg gaggcggcgg tgaaggctcg gtcgacgggg cacccggggc
     3001 cgcggctggt gcggtcggtg ctccagcgtt gctggcctcg ttcgagttcg gcgtctcggg
     3061 cggggtgacc acgatgggat tggaagcggc cggagtcggc gatgctgcgc ccggtgcatt
     3121 cgagtgctcc gcgcacgcaa caatgatcaa catgggtgcg agagtcagcg ctttcaccgt
     3181 gtccacgtac ttctccatct cgtcgtcgcg aattccaccc tagcgcgaat tacgcgactc
     3241 gccgagcaca ttcggctcac cgaagcgttc cgaatcgagt tcatcgtcga ggcgctgctc
     3301 ccgcagcgag tccaggcgat tgcgcaggtc caggtcgcct ggacgaagct cgagcgccac
     3361 acgcacgtgc tcttcggcgc taccgagttt gccgaggtgt cgcagcgcct caccgagacc
     3421 ggtatgggcg cgcacagagc cgggatttct cgcgagcgcc gcttcgaacg cggcacgcgc
     3481 tcgcggggac tcacccgctt cgagcgcgac attgccgagc gcgacgaagc tcgcgacgtc
     3541 tccaccgagc gccacgcttc gctccagcat ttgcaacgcg cccgtcaagt cgccggcgtc
     3601 ccgtcgcgcg agcccaagct ccagcgcgta ttcgctcgat gcgggcgcga gccgaacagc
     3661 gcgctcgagc aacttgcggc gcccttctgg catctccgag cgcgcggcgg tcgtgaggcg
     3721 cgaagcgaac caaccgccga acgactgcgc gagcaagagc gcacctcccg caccgagcaa
     3781 caagacgagc agacgcttgc gcgcgagcgg acgttctcgt ggcagagccg cgaaaataac
     3841 ggcgacgaga acgcacaccg ccggaaggtg gaatgggctg tctcccagcg cacaaacggc
     3901 gagcgcgacc agtgccgcgg ccgggtgggg ctcgcttgct cgagccgcca tcgcgatggc
     3961 gatgagcagc aacaacggag gcagcgggcc cgcttcgacc agaagctcga gccaatcgtc
     4021 gtgcgccgtg gt
//
LOCUS       DASTKS010001042         3953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_611122, whole genome shotgun sequence.
ACCESSION   DASTKS010001042 DASTKS010000000
VERSION     DASTKS010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611122"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..898
                     /locus_tag="VFQ35_22005"
     CDS             185..898
                     /locus_tag="VFQ35_22005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HET9933401.1"
                     /translation="MTPQTVISEEALRGGQMWSRVLRRGQVLRLTDVEGGASVAALFY
                     NADDKNERYNMPDTLKAQHIARLTAGYVLYSDMGRVLCSVVADSVGWHDTVTGHGRAE
                     HNLAKYGDGSYQKLRNDFYRNTRDNFLIELGKYELGKRDIVANVNLFVKVVVDAEGAL
                     RWEPNSKAGDFVELRAELNTLVVLSNTPHPLDPSPTYAPKPVALSVLQRGPAGPEDPC
                     RLSRPENARGFALTEAYFS"
     gene            911..1549
                     /locus_tag="VFQ35_22010"
     CDS             911..1549
                     /locus_tag="VFQ35_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HET9933402.1"
                     /translation="MALLESKLDPARARHDEVIESGKGWTHVVRKGQKFRIVDLEGNQ
                     AVDTLFYNADDPTDRYSATDTIREQGNIYLSTGTRILSSEGNVLMTIVADTCGRHDTL
                     GGACAAESNQVRYALEKKYMHSCRDSFLCTLACCTRDMSKRDLTANVNFFMNVPVTPE
                     GKLTFEDGISAPGRYVELSAAMDVRVLISNCPQLNNPCNGYNPTPIRVLIWD"
     gene            1744..>3953
                     /locus_tag="VFQ35_22015"
     CDS             1744..>3953
                     /locus_tag="VFQ35_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HET9933403.1"
                     /translation="MFERVLIANRGEIACRISRTLRKLGVEPVAVYSEADRHSRHALE
                     AAHAVCIGPAPAKESYLDAERVLKAALDTGAQAIHPGYGFLSENAAFAEACEARGIAF
                     IGPTPEQMRAFGLKHSARELAQRANVPLLPGTGLLRDAAHALSEAERIGFPLMLKSSA
                     GGGGIGMQLVRHEVELENAFEAVQRLSRNNFGDASVFLERFVARARHLEVQLFGDGRG
                     QVVALGERDCSAQRRNQKVVEETPAPRLAPELRSALLDAALRLGKSVSYRSAGTVEFV
                     FDADRNEFYFLEVNTRLQVEHGVTEAVTGVDLVEWMVRTAAGDPPDLRAPIAPRGHAI
                     EVRVYAEDPARDFKPSAGLLTEVEFPNDVRVDTWIERGTEIPAFYDPMLAKLVVHGDT
                     REQAVARLSAALEETRVGGVETNVAYLRELAKSHEFTRGEVLTSTLRHFEHKSRSIEV
                     IAPGTFSIIVDHPGRLGYWDVGIPPSGPMDSLSHRLANRAVGNDERAATLELTMSGPT
                     LRFATDACVALAGARMSAELDGTAVEFGVPLEVRAGQTLKIGAVSGAGTRTYLAVRGG
                     LDVPLYLGSRATFTLGGFGGHGGRTLRRGDVLHIGGLTPTSVPHALPPTLVPAFERRW
                     EIGVLYGPHAAPDFFTPRDIEMFFSTDWEVHYNSNRTGVRLVGPKPEWARSDGGEAGL
                     HPSNIHDVAYAIGAIDFTGDMPIVLGPDGPSLGGFVCPATVASAELWKVGQLRPS"
BASE COUNT          726 a         1307 c         1273 g          647 t
ORIGIN      
        1 acgaaccttt tggaagtaat gcaactcgtg agcctcaccg cacggcggta gtcgtcgctt
       61 cagatctcag tcttcgtcat tcaaatcgtt cggtgccgcg ccaatcgcgc gggcccgtac
      121 agttccaagc gactcatctg ctcgatgagg tgcggcgtca cgagttctgc tctggagaga
      181 actcatgact ccccaaaccg tcatttcgga agaagcgcta cgcggcggcc aaatgtggtc
      241 tcgcgtgctg cggcgcggac aggtgctgcg tctcacggac gtcgaaggcg gcgccagcgt
      301 ggccgcgctc ttctacaacg ccgacgacaa gaacgagcgc tacaacatgc ccgatacgtt
      361 gaaggcgcag cacatcgcgc gcctcacggc gggctacgtc ttgtactcgg acatgggccg
      421 cgtgctgtgc tcggtagtgg ctgacagcgt cgggtggcac gacacagtta cgggtcacgg
      481 tcgcgccgag cacaacctcg caaagtacgg ggacggctcg taccagaagc tccgcaacga
      541 cttttaccgg aacacgcgcg acaatttctt gatcgagctc ggcaagtacg agctcggcaa
      601 gcgcgacatc gtggcgaacg tgaacttgtt cgtgaaggtc gtcgtggacg cggagggagc
      661 cctgcgctgg gagcccaact cgaaggcggg cgacttcgtg gagctgcgcg cggagctgaa
      721 cacgctcgtc gtgctctcga acacgccgca cccgctggat ccaagcccca cgtacgcgcc
      781 gaagccggtc gcgctcagcg tgctgcaacg cggccccgct ggccccgaag acccgtgccg
      841 gctctcgcga cccgaaaacg cgcgcggctt cgcgctcacc gaggcctact tttcctgaaa
      901 ggaccgcgcc atggctctct tggaaagcaa actcgacccc gcccgggccc gccacgacga
      961 agtcatcgag tcgggtaaag gctggaccca cgtcgtgcgc aaaggccaaa agttccgaat
     1021 cgtcgatttg gaaggcaacc aagccgtcga cacgctgttc tacaacgcgg acgatccgac
     1081 cgaccgctac agcgcgaccg atacgatccg cgagcaaggc aacatctatc tttcgaccgg
     1141 aacgcgtatc ctctcgagcg agggcaacgt gctcatgacg atcgtggccg acacctgcgg
     1201 ccgacacgac acgctcggcg gggcttgcgc ggcggagtcg aaccaggtgc gctacgccct
     1261 cgagaagaag tacatgcaca gctgccgaga cagcttcttg tgtacgctcg cgtgctgcac
     1321 gcgggacatg agcaagcgag atctcaccgc caacgtcaac ttcttcatga acgttccggt
     1381 gactcccgaa gggaaactca cgttcgaaga cggaatctcg gcccccggca gatacgtcga
     1441 gctcagcgcc gcgatggacg tgcgggtgct catctcgaac tgcccgcagc tcaacaaccc
     1501 ttgcaatggc tacaacccaa ctcccatacg tgtcttgatt tgggattgaa cgactgaacg
     1561 tccctgcgcg agcgcgggga acgtagaagc ccgtgggccc ggaccatcgg atcactcggc
     1621 gcagggattg agcccccggc gtcgagcccc gaaaatcccg gtgagtctgc gaagcggttc
     1681 gggtcctcgg gcttcctcgt tccccgccct ctcgcgctgg ctgcgtcgag tggagtaacg
     1741 aacatgttcg aacgtgtgct catcgcgaac cgcggtgaaa ttgcgtgccg catcagccgc
     1801 acgctgcgca agctcggggt cgagccggtc gccgtctatt cggaggccga tcgccactcg
     1861 cggcacgcgc tggaggcagc gcacgcggtg tgcatcggtc ccgcgcccgc caaagagagc
     1921 tacctcgacg ccgagcgcgt tttgaaagca gcgctcgaca ccggtgccca ggcgatccat
     1981 ccgggctacg gatttttgag cgagaatgca gccttcgccg aagcgtgtga agcgcgcggg
     2041 atcgcgttca tcgggccgac tccggaacag atgcgagctt tcggcctcaa gcacagcgcc
     2101 cgcgagctcg cccaacgcgc caacgtaccg ctcttgcccg gcacgggact attgcgggac
     2161 gccgcccacg cgctcagcga ggccgagcga atcggcttcc ctttgatgct gaagagctcg
     2221 gccggcggcg gcggaatcgg tatgcagctc gtgcgccacg aggtggagct cgagaacgca
     2281 ttcgaggccg tacagcgcct gagtcgcaac aacttcgggg acgcgagtgt ctttctcgaa
     2341 cgcttcgtgg cgcgcgctcg gcacctcgag gtgcaactgt tcggggacgg gcgcggacag
     2401 gtggtcgcgc tcggcgagcg agactgttcc gcgcaacggc ggaatcaaaa ggtagtggaa
     2461 gagacgcccg cgccgcggct cgctcccgag ctccgcagcg cgttgctcga cgccgcgctt
     2521 cgcctcggca aaagcgtgag ctaccgctcg gcggggacgg tggagttcgt cttcgacgcc
     2581 gatcgcaacg agttctactt cctcgaggtg aacacgcgct tgcaggtcga acacggtgtg
     2641 accgaagcgg tgaccggggt cgacctggtc gaatggatgg tgcgcaccgc ggcgggtgat
     2701 cctccagatc tccgcgctcc aatagcgccg cgcggtcacg ccatcgaggt tcgcgtctac
     2761 gctgaagatc cagcgcgcga cttcaagccg agcgccggcc tcctcaccga agtggagttt
     2821 ccgaacgacg tgcgcgtcga cacttggatc gagcgcggaa cggagatccc cgccttttac
     2881 gaccccatgc tcgccaagct ggtcgtccac ggagacacac gcgagcaagc ggttgcgcgc
     2941 ttgtctgccg cgctagaaga gacccgcgtc ggcggcgtcg aaacgaacgt cgcctacctg
     3001 agagagctcg cgaagagcca cgagttcacg cgcggcgaag tgctgacatc gacgctgcgt
     3061 cacttcgagc acaagtcgcg atcgatcgag gtcatcgcgc cgggcacgtt cagcatcatc
     3121 gtcgaccatc cggggaggct tggttactgg gacgtcggca ttcccccatc gggcccgatg
     3181 gactcgctgt ctcatcggct agcgaaccgc gccgtgggca acgacgaacg cgcggcgacg
     3241 ctggagctca cgatgtccgg cccgaccctg cgctttgcga ccgacgcctg cgtcgcgctg
     3301 gcgggcgcac gcatgagcgc tgagctcgac ggcaccgcgg tggagttcgg cgtaccgctg
     3361 gaggtgcgcg ccggtcagac gctgaaaatc ggcgccgtga gcggcgccgg aactcgaacc
     3421 tacctcgccg tgcgcggcgg cctcgacgtg ccgctgtatc tagggagccg cgccacgttc
     3481 acgctcgggg ggttcggcgg tcacggcggg cgcacgctac ggcgcggcga cgtcctgcac
     3541 atcggcgggc tcaccccaac gagcgtaccg cacgcgctac ctcccaccct cgtcccagcg
     3601 ttcgagcggc ggtgggaaat cggcgtgctc tacggtccgc acgccgcgcc cgactttttc
     3661 acgccgcgag acatcgagat gtttttcagc accgactggg aggtgcacta caactcgaac
     3721 cgcaccggtg tgcgcctggt cgggcccaaa ccagagtggg cgcgcagcga cggaggcgaa
     3781 gccgggcttc atccgtcgaa cattcacgac gtcgcctatg caatcggcgc catcgacttc
     3841 acgggggaca tgcccatcgt gctcggtccg gatggcccga gcttgggcgg cttcgtgtgt
     3901 ccggcgacgg tcgcctcggc cgagctttgg aaggtcggac aactgcgacc ctc
//
LOCUS       DASTKS010001043         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1506312, whole genome shotgun sequence.
ACCESSION   DASTKS010001043 DASTKS010000000
VERSION     DASTKS010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506312"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1342)
                     /locus_tag="VFQ35_22020"
     CDS             complement(161..1342)
                     /locus_tag="VFQ35_22020"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9933404.1"
                     /translation="MNSRSLRSVLVCQLAAALLMGACSSGSNGQSGENGGSLAATGGV
                     STGGDSMRGGTAGAGASTSSQGGAASSSGGASGTHASGGSTSGGTSPTGGTVAFGGTS
                     ASSGGIGSTSGGRNGGGAPNGGRTGGGMTGGTGGASSAAGGTGGRSSSSSGGAGGSTA
                     PAGKAPYKGVANSPAAQLSALGATWCYNWGTTPKATDCNDPLFVPMVWGKDNVESAVQ
                     AIGKAGYTTLLGFNEPNKSDQANMSVAEALALWPTLTSNPNIRVGSPAVSDDGRAWLE
                     DFMTQAKKQGLRVDFIAMHWYGWNKGSCTAGSLEGAINWASKWNLPVWITEFGCMGSS
                     NPDEQTLLDFFKNAIPMIAKHSLVERYAWYPWNTYNHLYENNAITALGQAFAAAPQYR
                     Q"
     gene            1510..2151
                     /gene="trmY"
                     /locus_tag="VFQ35_22025"
     CDS             1510..2151
                     /gene="trmY"
                     /locus_tag="VFQ35_22025"
                     /inference="COORDINATES: protein motif:HMM:NF002560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HET9933405.1"
                     /translation="MRSFIVIGRTATASPDFSLEDLPSSSGRLDVLLRCIRAALLYSH
                     GLRAEVRVYLVLRGGPLAPRVLRIEAATARFIRPDERSLALLVKKTIAAANDGLGREF
                     AEIRPGVSLARGDVDCVISDLGGARLYLLDEQGSDVRESGIGDQRENTDSAYFLGDHL
                     GIEPKDRDHLASLGAMAISVGPRSLHSDDAIVVLWNELDRREAPHGDRASTRG"
BASE COUNT          433 a          842 c          823 g          466 t
ORIGIN      
        1 aagtgcaaca tcgcgctcgc attgaccgtg ctgcccgact cggccaacga cgtcaccagc
       61 aagagatccg acacgtcgac cgaagggcct ttgctccgta tgctcgccac gcatgagagc
      121 tagcactcgc tatgcgccct cgaaaggcga acgagcgcga ttactggcga tactgtggcg
      181 cggccgcgaa tgcctgcccg agcgcggtga tggcgttgtt ctcgtacagg tgattgtacg
      241 tgttccaggg ataccaggcg tagcgctcga cgagggagtg cttggcaatc atggggatcg
      301 cgtttttgaa gaagtcgagc agcgtttgct cgtcggggtt actcgagccc atgcagccga
      361 actcggtgat ccaaaccggg aggttccatt tcgatgccca attgatggcg ccttcgagcg
      421 aacctgcggt acacgagccc ttgttccacc cgtaccagtg catggcgatg aagtcgacgc
      481 gtagtccctg cttctttgcc tgggtcatga agtcttcgag ccaggcgcgg ccatcgtccg
      541 agacggcggg gctcccgacc cgaatgttcg gattgctcgt gagtgtgggc cagagcgcga
      601 gcgcttcggc gacggacatg ttcgcttggt ccgacttgtt cggttcgttg aagccgagca
      661 gcgtcgtata gcccgccttg ccaatcgctt ggaccgcact ctcgacgttg tccttgcccc
      721 aaaccatcgg cacgaacagc gggtcgttgc agtcggtggc cttcggcgtc gtgccccagt
      781 tgtaacacca cgtggcgccc agggcggaga gctgcgcggc gggggagttg gcgacaccct
      841 tgtagggcgc tttgcccgcg ggcgcagtgc tgccaccggc accaccgctg ctcgaactcg
      901 agcgacctcc agtgccacca gcggcgctcg acgcgccgcc agttccgccg gtcatgccgc
      961 ccccagttct gccgccgttg ggggctccgc cgccgttgcg gcctcccgac gtcgagccaa
     1021 tgccgccgct cgaggcactc gtcccgccga aagcgacggt tccgccggtg ggtgaggttc
     1081 cgcccgacgt gctcccgccc gaagcgtgcg tgccggatgc accgcccgag ctactcgctg
     1141 caccgccttg cgagctagtg ctggctcccg cgccagcggt tccaccccgc atggagtcgc
     1201 caccggtgga gaccccgccc gttgcggcga gagaaccccc gttttcgccg gactgcccat
     1261 tcgagccaga tgagcacgct cccatcaaaa gagccgccgc caattggcac accaggaccg
     1321 aacgcaagga ccgagagttc acgatacgtc cagtgtcgca tgcggtctcg cgccaggctc
     1381 agtcgggcga tgccgggcgc gccgcggttg agcgatttca tggcttggcg atccgcttca
     1441 tccgccaaga agctactttt ccgccgaggc tgacagctgc tcgagatcgt gcgcttatca
     1501 ccggcactca tgcggagctt catcgtcatc gggcgaactg ccacggcttc tccggacttc
     1561 tcgctcgaag accttccgag ttcgagcggc cggctcgacg tgctcttgcg ttgcattcgg
     1621 gccgcgctcc tctactcgca tggcctgcgt gcggaggttc gcgtttactt ggtgctccgc
     1681 ggaggtccgc tcgcgccgcg ggtgttgcgc atcgaagccg caaccgcgcg cttcattcgc
     1741 cccgacgagc gcagcctggc gctgctcgtg aaaaaaacta tcgctgccgc gaacgacggt
     1801 cttggtcgcg agttcgcgga gatcaggccc ggagtctccc tcgcgcgtgg cgacgtcgat
     1861 tgcgtcattt cggatctcgg cggagcgcgg ctctacctgc tcgatgagca aggttcagac
     1921 gtgcgagaga gcggcatcgg tgaccagcgc gagaacaccg actccgcgta cttcctcggc
     1981 gatcacctgg gcatcgagcc caaggaccga gaccaccttg cgtcgctcgg cgcgatggca
     2041 attagtgtcg gcccgcggag ccttcactcg gacgacgcaa ttgtcgtcct ctggaatgaa
     2101 ctggatcgtc gcgaggcccc gcacggagac cgagcgagca cgagagggta gccgattcgg
     2161 cgatcagcgc gagcgcgagc gggtagatga ttgcgcgagg tgggcgagcg cgggaaggta
     2221 ggggcctcac gcgcccgcct cgccgcggtc tcggctcgct cgcgactgac ttatccattc
     2281 gcgggccctc taagctatcg gcgaaacatt gtgacaaaaa gtgtcaaaac gtcacaacgc
     2341 cctgggaaac gaggtcgacg cggtcgacgc gcgaagtcat cgctgggatc gacgcgcgaa
     2401 tcatcgctcg gatcgacgcg cgaatcatcg ctcgggtcga cgcgcgaatc atcgctcggg
     2461 tcgacgcgcg aagtcatcgc tcgggtcgac gcgcgaatca tcgctcgggt cgacgcgcga
     2521 atcatccctc gtggctggct gaggctcgac agctcaacga aagc
//
LOCUS       DASTKS010001044         7925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1728071, whole genome shotgun sequence.
ACCESSION   DASTKS010001044 DASTKS010000000
VERSION     DASTKS010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728071"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..1921
                     /locus_tag="VFQ35_22030"
     CDS             479..1921
                     /locus_tag="VFQ35_22030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933406.1"
                     /translation="MPVAAGGMAMVWAARLKGTRGFQKIVAVKTMLPKLSEEVQFEQM
                     FLDEASLASQIRHPHVVEILDLGEQDGVLFLVMEWIDGMPLNQLMKAAKIAGGMPLPI
                     ATRIVMQACAGLHAAHELRDSKGQLVGLVHRDVSPQNILVTYDGVTKVVDFGVAKATA
                     HGGGSTSAGQIKGKVGYMAPEQIEGEEAMDRRADIFAMGIVLYALTTGKHPFRRESDA
                     ATMYNICSPAPAVPPRKFLPNYPESLERVLLKSLAKSPSDRYETANDMLRDLDQALPA
                     NQRVITDEEVAHFVRKLVGDKLQERRATLSDALMRADSLAPLRDADLSSATGSVSHGG
                     VMSQSLLPPPPPDNATTTELSGLGYGRKRTLLIGGVAVALGAAALAITLMSRTPEPVS
                     APAAATPLPTAVPVLVAPPTPPQTPTPSPATSADKPAPAPTETKAGTKPLQQGGGKKP
                     GPGGKPTQPPVTAPVKPPPPQNDWRQNPGF"
     gene            complement(2097..4544)
                     /locus_tag="VFQ35_22035"
     CDS             complement(2097..4544)
                     /locus_tag="VFQ35_22035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9933407.1"
                     /translation="MARTDSARESICQLLTSGEVRYELVQAETSEAASEMLERTPRGN
                     FACILFDARTSASDTAHALQSLLDSNGQRRLPVIVILNENDEWSELSRLGVTDYVRTG
                     WLNAESLGRAVEHALENERLTLRVRNAEARLQSVVSCLTDPVGVGASRVAPGLPKDQP
                     KTCFDCEWLHDSRRIGERAREEVVRDPSAAVRALAAGEARLRLALEASSTGLWTWDVA
                     DDAVTWSPECYSISGLKRGEFAGTGAAFFELVHPEDRERVREKVLGAISNQSLYQCVF
                     RIVRPNREIVWVENRGRASYAPSGMPLEMLGTITDVNARVHAEQAARRAEALAQAATN
                     GADALVFAKDLGGRYFFFNDKWLEFCGLTRDQAAGLTDAQLFGSDAEGLLANDSEVVR
                     TGKAVRFEERIQHDGSDVTLLSSKFPLVDAEGKLFGLCGVSVDISELKRLQAALRARE
                     RELQTLADNIPDILARFDRGLRHVYVNASVEAATGRPRADFLLKTNRELGMPEDLCAV
                     WDAALTRVFESGEPTSIEFSFEQGGERSFYESRLVPEANESDEVEYVAAITQNITVRH
                     DYEQALLEADRRKDEFLATLAHELRNPLAPVRTGIDVLKHGADPRVAQNTLRMMDRQL
                     QQFIRLIDDLLDVARITTGKISLRRERTALATVVDAAIEAVKPKIDSSGHALSVELAA
                     EPLYVVADSARLTQVLSNLLTNAAKYTPRGGAIRVTLRREADQAVVDVADNGVGIPRE
                     MLHRVFEMFTQINRSIEQSQGGLGIGLSLVKRIVEMHGGSVTASSAGAGLGSCFTVML
                     PLESALPAPSPESKAAR"
     gene            complement(4792..7224)
                     /locus_tag="VFQ35_22040"
     CDS             complement(4792..7224)
                     /locus_tag="VFQ35_22040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9933408.1"
                     /translation="MVRAITATGLKAIAASTEELLLLVSSVKDYAIFMLDPGGYVNSW
                     NAGAGALKGYTEAEILGSHFSVFYPLNERAQRKPELALEAAARDGRYEDEGYRVRKDG
                     SRFWASAVITALRDDQGRLRGFGKVTRDLTERKQREDELRRYNAELERRVEERTRELI
                     DASRDAQSAIAAVHRMMKDVRESERRHRTLVEHMNAGVLALDSEGNTTFMSARLGRML
                     GREPRDSLGRPAVSFIVSAQRSRALEVMQSHGTIEVQAERDGLSPIWVSLECVPLTQD
                     DGEPGGVVCLVTDLSAQRNADESIDRFAAIVDAAESAVFSVDRDRRITSWSRGAEALF
                     GYTKAAAVGMLVDALYPTSISGELERVIDDLSAKPKGARDVSTQVREDGSTVDVSVIT
                     YPIKVATGDAELSIVMRDLTAQRRADATLRQVTEQVQQVQKMEALGRLAGGIAHDFNN
                     MLSVIMSYAELLHEALGADAPLAGDVREIQQASQRAAALTRQLLMFSRGQLVTRSVVE
                     LNPLVDAMVEMLKRTVGEDVELSLSLADQLWPVRIDPNQFQQVILNLAVNARDAMPSG
                     GVMSICTRNVSLDTTYVDTHFPARPGDYVMLSMTDTGVGMDAATREHIFEPFFTTKGP
                     GKGTGLGLATVFGIVQQARGLIWVYSEPGNGTVFKVYLPRSEVGREQPVSEPQPALAR
                     GSETILLVEDEAAVRSAARVSLASNGYRVLEAPTPQAALDIAKAHPDEIHLLLTDVVM
                     PEMNGPQLAQIVASLRPSMRILFMSGYAEDRMLPHGLVAHAFSLLEKPFTSSSLVRKV
                     RETLDAERLRPD"
BASE COUNT         1390 a         2538 c         2496 g         1501 t
ORIGIN      
        1 cggcacgacg cggagcgcca aagtggcggc tcgcttcagc tccgcgcggc gttggcgtag
       61 gaaggcggac gaccacattg gggttggatt tagtacctgc gcggacgtcg tgtgcggctt
      121 ttcggctatg gccggattca cgccgccaag ttacgacgca cgacgtcaga ctttcccgtg
      181 cttatagaaa ttgggccgct tcgcccgaga acggctatgt cgcccccgaa caatcgcgcg
      241 tgctcgtgtc tcaaacccag accgacggac caggaaagtg ctaaatgata gtgttgttct
      301 tcgagtccga cgttaccgtg cggctgtcat gcgagagagg ccacacgccg cttgagaccc
      361 gcgtcctcct ttgcaacact ccaacctaac tccagtgact tcggcgatcg aacctgcccc
      421 gctgaaaatg tccgggatgg tcgccccggg gcaagtgctt gggcggtacg aactgctcat
      481 gcctgtcgcc gctggcggga tggccatggt gtgggcggcg cggctgaaag gcacgcgcgg
      541 gtttcagaag atcgtcgccg tcaagacgat gctgccgaag ctgagcgaag aggttcagtt
      601 cgagcagatg tttctcgacg aggcatcact agcctcgcag atccgccacc cgcacgtcgt
      661 cgagatcctc gatctcggtg agcaagacgg cgtccttttt ctggtgatgg agtggatcga
      721 cgggatgcca ctcaaccagc tcatgaaggc ggcaaagatc gccgggggca tgccgcttcc
      781 catcgcgacg cgcatcgtca tgcaggcgtg cgcgggcctg cacgctgctc acgagctgcg
      841 cgattcgaaa gggcaactcg ttggcctcgt tcatcgcgac gtgtcccctc aaaacatttt
      901 ggtgacctac gacggcgtca ccaaggtcgt ggatttcggc gtcgcgaagg ccaccgcgca
      961 tggcggcggt tcgacgtcgg cggggcagat caaaggcaaa gtcggctaca tggcgcccga
     1021 gcaaatcgag ggcgaagagg ccatggacag gcgcgccgac atcttcgcga tgggcatcgt
     1081 cctgtacgcg ttgacgacgg gtaagcatcc gttccgtcgc gagtcggatg ccgccacgat
     1141 gtacaacatc tgttcgcctg cgccggcagt acccccccgt aagttcctgc cgaactatcc
     1201 ggagtccctc gagcgcgtcc ttttgaagtc gctcgccaag tctccctcgg atcgttacga
     1261 gaccgcgaac gacatgctgc gcgatctcga tcaggcgctg ccggcgaacc agcgtgtcat
     1321 taccgacgaa gaggtcgcgc atttcgtgcg caagctcgtc ggcgacaagc tgcaagagcg
     1381 ccgcgcgacg ttgagcgacg cgctgatgcg cgccgactcc ttggcgccgc tgcgcgatgc
     1441 cgacctcagc tccgcgacag gctccgtctc ccacggtggc gtcatgtcgc agagccttct
     1501 gccgccgccg ccacccgaca acgcgacgac gacggagctc tccggcctcg gctacgggcg
     1561 gaagcgcacg ctgctcatcg gtggagtagc ggttgcgctc ggcgccgcgg cgctcgccat
     1621 cacgctgatg tcgcgcacgc ccgagcccgt gagcgcaccg gcggcggcga cgccgcttcc
     1681 caccgctgtt ccggttttgg tggcgccccc aacgcctcca caaacgccaa cgccttcgcc
     1741 agccacttcc gccgacaagc ccgcgccggc gccgaccgaa acgaaggcgg ggacgaagcc
     1801 tctccaacag ggcggtggca agaagccggg tcctggcggc aagcccactc agccgccggt
     1861 gacagcgccg gtcaaaccgc ctcctcccca gaacgactgg cgtcaaaatc ccggatttta
     1921 gcggcttcgc gtgcgctacc gcgggtcggg gcgggcgcac gagcggttga agagatgcga
     1981 tcagcttggt gagaacgcca gcgcgctaat caacggcccc ctgagcgtgg acccctcgag
     2041 aacagcgtgg acccctcgag aacatagagg cagccgaagc gcggcgagta ggcgctttac
     2101 ctggcggcct tagactcagg tgagggtgct ggcaacgcgg actcgagcgg caacatgacc
     2161 gtgaagcaac tgccgagccc cgccccggcg ctcgaagcgg tcacgctgcc accgtgcatt
     2221 tctacgatcc gcttgactag cgaaagcccg atgccgagtc cgccctgcga ctgttcgatc
     2281 gagcgattga tttgcgtgaa catctcgaac acgcgatgga gcatctcgcg cgggatgccg
     2341 accccgttgt cggcaacatc gacgacggcc tggtcggcct cacgacgaag cgtgacccga
     2401 atcgcgccgc cgcgaggcgt gtacttggcc gcgttggtca gcaaattgct gagtacctgg
     2461 gtcaagcgcg ccgagtcagc gacgacgtaa aggggctcgg cagccagctc gacggagagg
     2521 gcatgtcccg acgaatcgat cttgggtttt actgcctcga tcgcggcgtc gacgacggtg
     2581 gcgagggccg tgcgctccct ccgaagcgaa atcttgccgg tcgtgatcct cgcgacgtcc
     2641 aggaggtcgt ctatcaaccg gatgaactgc tgcagttggc gatccatcat gcgcagggtg
     2701 ttctgcgcga cgcgcggatc ggcgccgtgt ttcagcacgt cgatcccggt cctgacgggg
     2761 gcgaggggat tgcggagctc gtgcgcgagc gtcgcgagga actcgtcctt acggcgatcg
     2821 gcctcgagca gcgcttgttc gtaatcgtgc cgcacggtga tgttttgcgt tatcgcggca
     2881 acgtactcga cttcgtcgct ctcgttggct tcggggacga ggcgcgactc atagaacgag
     2941 cgctcgccgc cctgctcgaa cgaaaactcg atgctggtcg gttcccccga ctcgaagacg
     3001 cgggtcaaag ccgcgtccca gaccgcgcac aaatcttccg gcatgccgag ctcgcggttc
     3061 gtcttcagca ggaagtcggc gcgcgggcgc ccagttgctg cttctaccga agcgtttacg
     3121 tatacgtggc gaagtccccg atcgaagcgc gcgaggatgt cgggaatgtt gtccgcgagc
     3181 gtctgtaact cgcgttcgcg cgcgcggaga gccgcttgca agcgcttgag ctcgctgatg
     3241 tcgaccgaca cgccgcacag cccgaagagt tttccttccg cgtccaccag gggaaacttg
     3301 ctcgaaagca aggtcacgtc ggagccatcg tgctggatgc gttcttcgaa gcgtacggct
     3361 ttcccggtgc ggacgacctc cgagtcgttc gccaaaaggc cctccgcgtc gcttccgaac
     3421 agctgcgcat cggtcagccc cgcggcttgg tcacgggtga gcccacagaa ctcgagccac
     3481 ttgtcgttga agaagaagta gcggccccct aggtctttgg cgaagaccaa agcgtcggcg
     3541 ccgttcgtcg cggcttgcgc gagcgcctcc gcgcggcgtg cagcctgctc tgcgtgcacc
     3601 cgcgcgttga cgtcggtgat cgtgcccagc atctcgaggg gcatcccgga aggagcgtaa
     3661 ctcgcgcggc ctcggttctc gacccacacg atttcgcggt tcggtcgcac gattcgaaat
     3721 acgcattggt acagggattg gttgctgatt gcgccgagca ccttctcacg cacgcgctcg
     3781 cgatcttccg ggtgcacgag ctcgaagaac gctgcgccgg tgcccgcgaa ttccccgcgt
     3841 ttcaacccgc tgatgctgta gcactcgggc gaccacgtca cggcgtcgtc tgcgacgtcc
     3901 caagtccaca accccgtcga agacgcctcg agagcgagcc gtagccgcgc ttcgccggcg
     3961 gccaaagctc gaaccgccgc gctcggatcg cgcactacct cctcgcgcgc gcgttccccg
     4021 atacgccgac tatcgtgaag ccactcacaa tcgaagcacg tcttcggctg gtctttgggc
     4081 agccctggag ctacgcggga agcaccgact ccgacgggat ccgtcaagca cgagaccacg
     4141 ctttgcagcc gggcttctgc gtttcggacg cgtagtgtga gccgctcgtt ctcgagcgca
     4201 tgctccacgg ctcgcccgag gctctctgca ttgagccagc cggtgcgcac gtaatccgtg
     4261 acgccgaggc gtgaaagctc gctccactcg tcgttctcgt tcaatatgac gatcaccggc
     4321 aacctgcgtt ggccgttcga gtcgaggagg ctctgtaaag cgtgagcggt gtcggatgca
     4381 ctcgttctcg cgtcgaacag aatgcatgcg aagttccccc gaggcgtgcg ctcgagcatt
     4441 tccgacgcgg cctcgctggt ctcggcttgg acgagttcgt atcgaacttc gccactcgtc
     4501 aggagctggc agatgctttc tcgcgccgag tcggtgcgag ccacgaccag gaccgagact
     4561 cccgtcgtcc gggcctcgtc gttagtgcct ttcaggggaa gtctcccggg gcgccaccga
     4621 gggtccaacc caaacgactc gcgcgtaccg agcgcgcaca tctgcccaag agttttgcaa
     4681 cgcgctatcg agttcgatcg tcgactcgag ggccgttgtt gctacgttgc aaaaatgcca
     4741 catctgcccg gtgcgaagct gagcagaagg taagtaatct gtacgagttc gtcaatcggg
     4801 gcggagccgt tccgcatcga gcgtttcgcg cacttttcgg actagggacg acgaagtgaa
     4861 cggcttttcg agaagcgaga atgcgtgtgc cacgagcccg tgagggagca tgcgatcttc
     4921 cgcgtagcca gacatgaaga ggattcgcat ggacggtcgc aagctcgcta cgatttgcgc
     4981 gagctggggg ccgttcattt cgggcattac cacgtcggtc aggagcaggt gaatttcatc
     5041 cgggtgagct ttcgcgatgt cgagcgcggc ttgtggggtt ggcgcttcga gtactcgata
     5101 gccgttgctg gccaagctga ctcgggcagc tgacctcacc gccgcttcgt cctcgaccaa
     5161 caatattgtc tcactgccac gggcaagcgc aggctgaggc tccgaaaccg gttgttctcg
     5221 gccgacttca ctgcgcggca agtagacttt gaataccgtg ccgttccccg gttcgctgta
     5281 cacccagatc aacccgcgcg cctgctggac tatgccgaac acggtcgcaa ggccgaggcc
     5341 ggtgcccttc cccggaccct tggtcgtgaa gaatggctcg aagatgtgct ctcgcgtggc
     5401 ggcgtccatg cccacgcccg tgtccgtcat gctcagcatg acgtagtcgc ccggccgcgc
     5461 cgggaagtgc gtgtcgacgt aagtggtgtc gagcgagacg ttccgagtgc aaatagacat
     5521 cacaccgccg ctcggcatgg cgtcgcgcgc gttgacggca aggttcaaaa tcacttgctg
     5581 gaattggttg gggtcgattc gcactggcca caactggtcc gcgagagaaa gggagagctc
     5641 gacgtcctcc cccactgtgc gcttcagcat ctcgaccatc gcatcgacca gggggttgag
     5701 ctcgaccacc gacctcgtga ccagttgccc gcggctgaac atgagcagtt gccgcgtcag
     5761 agccgccgcg cgctgagagg cttgctgaat ttcgcgaacg tcgcccgcca acggagcgtc
     5821 ggcgccgagc gcctcgtgca ggagctcggc gtagctcatg atgacggaaa gcatgttgtt
     5881 gaagtcgtgg gcaatgccgc ctgccaagcg accgagcgcc tccatttttt ggacctgctg
     5941 gacctgctcc gtcacttgtc gcagtgtggc gtcggcgcgc cgctgcgcgg taagatcgcg
     6001 catcacgatc gagagctcgg cgtcgccggt cgcgaccttg atgggatacg tgatgacgga
     6061 cacatcgacg gtgctgccgt cttcgcgaac ttgcgtcgag acgtcgcgcg caccctttgg
     6121 cttggccgat aggtcgtcga tcacccgctc gagttccccg cttatgctcg tagggtagag
     6181 ggcgtcgacg agcatgccga cggcggcggc cttggtgtac ccgaacagcg cttccgctcc
     6241 tcggctccaa ctcgtgatgc gccgatcccg atcgacgctg aagaccgcgc tctcggcggc
     6301 atctacgatt gcggcgaagc gatcgatgct ttcgtccgcg ttgcgctgag cggaaaggtc
     6361 ggtgaccagg cacaccactc cgccgggctc gccgtcatct tgcgtcaagg gaacgcactc
     6421 gagtgaaacc caaatgggcg agagcccgtc gcgctccgcc tggacttcga tggtgccgtg
     6481 gctctgcatc acctcgagag cccttgatcg ctgggcggaa actatgaagc tgactgcggg
     6541 gcgacccagc gagtctcgcg gctcacggcc cagcatacga ccgagccgcg cgctcatgaa
     6601 ggtggtgttg ccctccgaat cgagcgccaa gacccccgcg ttcatgtgct cgaccaacgt
     6661 gcggtggcgt cgctcgctct cacgaacgtc tttcatcatt cggtgtacgg cggcgatcgc
     6721 gctctgggcg tcccggctcg cgtcgatgag ctcgcgggtg cgctcttcga cccgacgctc
     6781 gagctcggcg ttgtagcgtc ggagctcgtc ctcgcgctgc ttgcgctcgg tcaggtcgcg
     6841 cgtcactttg ccgaaccccc gcagccgccc ttggtcgtca cgcagagccg taatgacggc
     6901 actcgcccag aagcgcgagc catctttgcg aacgcggtag ccttcgtcct cgtagcggcc
     6961 gtcgcgcgcc gccgcctcca atgcgagctc cggcttgcgt tgggcgcgct cgttcaatgg
     7021 gtaaaacacc gaaaaatgac tgcctaggat ctccgcttcc gtatatccct tgagcgcgcc
     7081 cgcgcctgca ttccagctgt tcacgtatcc gcctggatcg agcatgaaga tcgcatagtc
     7141 tttgacgctg ctcacgagga ggagcagttc ttccgtgctc gccgcgatcg ctttgagccc
     7201 ggtcgccgta attgcccgta ccatgacgtc tcaccgcccg agagaacgag accgctggcg
     7261 cgaacgtgag agcgcactcg ttcatgcgac cacagacgcc cagttgctgg acctcggtca
     7321 tcgtagcagc gacgatttgc tattgcagta tctctgatca gcgcgtctcc tttttcagaa
     7381 ttggcgatct cgtacatggg gtgtcggaag tggcggaagc taataatttg aaggtgattc
     7441 agattcgcta tggcggaatc gaatcgacgc attgacccat cgtacgatga agtctcttac
     7501 tgttgcttcc gggtttcttt gtggcgatct cgtccaacca gatttgtccc atcggttgtg
     7561 ctcggcgacg caacggcgac ttcggcgtta cgcctgagct cgctcgactg gagagtccga
     7621 acaaacgacc ggcggtggct cggtcggtag aaaccttcta gagcgcgtag ccctcgccga
     7681 ggctgcgaag atacctattg acctcaaccg aataatccgt ccggctggga tcgccgtcga
     7741 gtgagtcgga cgtacccgcg ccgggtcgct ccgtgctggt tccgagcgaa gtcgatacgc
     7801 ccaagtgtgc gcccgaaagt cgtgaaagcg cgcggcgctc cggctttcgg ctcgatgtgt
     7861 tcgggccctt agcatgtggc gaatctgcac tgacggcgcg cccgggcgcg ttgagtcccg
     7921 ggcgg
//
LOCUS       DASTKS010001045         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_165262, whole genome shotgun sequence.
ACCESSION   DASTKS010001045 DASTKS010000000
VERSION     DASTKS010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165262"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1307
                     /locus_tag="VFQ35_22045"
     CDS             135..1307
                     /locus_tag="VFQ35_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9933409.1"
                     /translation="MRLWFSYGGKNRVVLEQLVRAFNESQGDVHVQAVFQGDYYEALA
                     KLRVAMAARDAPALSHVVGEIVPYLCEAGVLARFDRHEMQYARDVLPALGQSGSFRGG
                     AQKPLVALPFNRSTPIAYLNASMFREAALTPPRTWTELRETARSLTRSTAGRTTRYGF
                     GCPIDWWFWAALVGQAGGELTAPDGSVTLGGEAGLAALELWRSLVVTDGSMKPPPGRD
                     YNAWEQTNQDFLAGRVAMIWTSTAFLKYLEENANFEIWAAPLPMQARLSVPTGGTHWV
                     LPASAPAEERAAAARFVDFMLSPSGAASFATNTGYIPVTNGAVRLLEAEGFFAKRPNW
                     QVAMAQLAHAAPWPWSPRLFRIQREIVQPRLERAIFGGGAARALLDEARGLAREGT"
     gene            1307..>2089
                     /locus_tag="VFQ35_22050"
     CDS             1307..>2089
                     /locus_tag="VFQ35_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9933410.1"
                     /translation="MRPAHRLHPLALLGPTLAVLGLFYFYPLVLLGFYSLHEWDLLTP
                     PRYVGLANYAGLVKSGVLWDSLVTTLRISVSSVLGSTILGLALALAVNRPGRIASFVR
                     SSVFSAYVVSWVSVGLLWLWLLDGDSGLFATFLRPLLGRGRGPLADPTTAPWALSLVI
                     TWKITGYAMVLFLSGLQSIPSSVLEASALDGASAWARFRHVVLPLLSPTTAFVATTSL
                     ILTFQAFDVVRVMTQGGPVKSTTVFVYSIYEQMFMDLRVGRAS"
BASE COUNT          296 a          698 c          670 g          425 t
ORIGIN      
        1 tcgtctagca tggggagccg cccattatgg cccagcccct tccccgacgc gtcctttcgc
       61 gacgttcgtt cctgctttgc gccagcgcca gcgcgtgctc gcaggaaccg gaggtcggcc
      121 gcggacgggt tcgggtgcga ctctggttca gttatggagg gaagaaccgc gtcgtgctcg
      181 agcaactcgt gcgcgcgttc aacgagtccc aaggggacgt ccatgtgcag gccgtgtttc
      241 agggcgacta ctacgaggcg ctagcgaagc tccgcgtcgc gatggcagcg cgcgacgccc
      301 ctgccctgag tcatgtcgtc ggcgagatcg tcccgtactt gtgcgaagcc ggcgtgcttg
      361 ctcgcttcga tcggcacgaa atgcagtacg cgcgcgacgt gctgccggcg ctcggtcaaa
      421 gcggctcgtt tcgaggggga gcgcagaagc cgctcgtcgc gcttccgttc aatcgctcga
      481 cgccaatcgc ctacttgaac gcctcgatgt ttcgagaagc ggcgctgaca ccaccgcgta
      541 catggaccga gctccgcgag accgcgcgct ctctcacacg aagcactgcg gggcgcacga
      601 cccgctacgg cttcggctgt ccgatcgatt ggtggttctg ggccgcgctc gttggtcaag
      661 cgggcggcga gctcactgcg ccggatggca gcgtcactct cggcggtgaa gccgggctcg
      721 ctgcgctcga gctctggcgc tcgttggtag tgaccgatgg cagcatgaag ccgccgcccg
      781 gtcgcgacta caacgcctgg gagcaaacga accaggactt cttggccggc cgcgttgcca
      841 tgatttggac ctccacggcc tttctcaaat acctcgaaga gaacgcaaac ttcgagatct
      901 gggccgcgcc gctgccgatg caggctcgtc tctcggtgcc gacgggtggg acccactggg
      961 tgttacccgc ttccgcgccc gcagaagagc gcgcggctgc ggcacgtttc gtggacttca
     1021 tgctgagtcc gagcggcgcc gcgagctttg caaccaacac cggctacatc cccgtgacga
     1081 acggcgcggt ccgactcctc gaagcggaag gcttcttcgc aaagcggccc aattggcagg
     1141 tcgccatggc ccagctcgcg cacgcagcgc cgtggccgtg gtcgcctcgg ctcttccgca
     1201 tccagcgtga aatcgtgcag ccgcgactcg agcgcgcgat ttttggcggc ggcgcggcgc
     1261 gcgcactgct ggacgaggca cgcggtctcg ctcgcgaggg tacttagtgc gccccgctca
     1321 tcggcttcat cccctcgcgc tccttggtcc gacgctcgcg gtgctcgggc tcttctattt
     1381 ctaccccttg gttctgctcg ggttctacag cctgcacgag tgggaccttc tcactccacc
     1441 gcggtacgtt gggctcgcga actacgccgg cctcgtcaag agcggcgtgc tctgggactc
     1501 actcgtcacg acacttcgca tcagcgtaag ctcggttctc ggttccacga ttctgggcct
     1561 ggctctcgcg ctcgcggtga atcgacccgg gcgaatcgcg tcgttcgttc gcagcagtgt
     1621 cttcagcgcg tacgtcgttt cttgggtgag cgtcgggttg ctttggctgt ggctgctcga
     1681 cggtgactcg gggctcttcg cgaccttttt gaggccgctc ttggggcgag gccggggccc
     1741 tctcgccgac cccacgacgg cgccgtgggc tctgtctctg gtcattacct ggaagatcac
     1801 tggctacgcg atggtgctct tcctgagcgg gctgcaatcg atcccgtcga gcgtgctgga
     1861 agcctcggcc ctcgacggtg cttccgcctg ggcgcgcttt cgccacgtgg tcttgccgct
     1921 gctctctccc accactgcgt tcgtcgcgac gacgagcttg atcctcactt ttcaggcgtt
     1981 cgacgtcgtg cgcgtcatga cccagggcgg gccggtcaag tcgaccaccg tgttcgttta
     2041 ctcaatttac gagcaaatgt tcatggacct gcgagtgggc cgcgccagc
//
LOCUS       DASTKS010001046         7173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1952932, whole genome shotgun sequence.
ACCESSION   DASTKS010001046 DASTKS010000000
VERSION     DASTKS010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952932"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1468
                     /locus_tag="VFQ35_22055"
     CDS             233..1468
                     /locus_tag="VFQ35_22055"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9933411.1"
                     /translation="MECTRRALATVAILFLAAACRKGSEPQPEKLHRETSAAAPAKSE
                     TIRIGLIASLTGKYSALGSEDKKAVDLAVEQINQSGGLLGKKLEVLTRDDQTLPDKSV
                     LAYNDLKGQNVVAVIGSVFSNSALATLPLAQNDGMPYLSLTPAEEQVIPVKPYVFVIP
                     ALANAYAERYLEYMQARKIVKIAVAHDTKGAYAISGYNAMKTLAPKYGVEIVRDEVFE
                     TSTNDFSPIFTHLKGAPAQAFVFWGTGPSGVTVTKQYAASGLKVPLFMTPAQASKLWI
                     DPVGAAGEGVTVLSAIGVVGEYLPDGPQKQIIQRMAAPYQEKHGYPPPQFAQDGYSAC
                     LLLFEAIKKADSTERPKIQQALETLSMVTPNGAYKYTPTDHSGLTRDYISVNVVREGK
                     LVPTDWAKEKLARTVAEQH"
     gene            1477..2238
                     /locus_tag="VFQ35_22060"
     CDS             1477..2238
                     /locus_tag="VFQ35_22060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933412.1"
                     /translation="MLHVEGLSRRFGGVYAVQGVSFRVQPGELRGVIGPNGAGKSTLF
                     HLIAGHLKPSAGRITLGNEPIDRLRPDQRARRGISIVYQGDRIFRRMSVRENVMVGAH
                     CKARHGFFEAALRLPRCRREDRELHDKADAVLERVGLAAVAEQPAESLPLGQQRTMQL
                     ARALAADPKVLLLDEPAAGLRATERSQLARLIREVRAHGLTVLLVEHDVALVTTLSDT
                     ITVLDLGRVIAEGEPSAIRRDPKVLAAYLGEGPPS"
     gene            2235..2903
                     /locus_tag="VFQ35_22065"
     CDS             2235..2903
                     /locus_tag="VFQ35_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933413.1"
                     /translation="MIQVDELSVRYGAALALDGVSLNVAAGEKVALIGANGAGKSTLL
                     NALSGMVPKTAGNVRFGGRFAHVPEGRQLFPDLSVEDNLRLGAFRVRERNPAWIYELL
                     PDLGRLRRQRAGSLSGGQQQMVAIGRGLMAQPDVLAIDEMSLGLAPIIVQQLAERLSE
                     LNAARGLALLLVEQSARVALSLCERAYVLETGRVVAQGSASELADSPFVVSAYLGSPL
                     EGSS"
     gene            2900..3760
                     /locus_tag="VFQ35_22070"
     CDS             2900..3760
                     /locus_tag="VFQ35_22070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9933414.1"
                     /translation="MSAFLSYFFHGISVGCGFALIASGLIIIYRVTRVVNLAQGMFGV
                     VAGFSATTLLDLGLPHVLAELVAIAAAAGVGVATGYVAIGKRGTSPQASLIATLGIGV
                     FAYAVEILTWGDQPRSFAGYSGAFELAGVEMPKQYALVTVVTLLVFGGLEAFFERTYA
                     GKALTACASNPYAAALQGIEVRRMGLYGFALGGALGGVAGVLLTPLQPVSYDSDVLLF
                     VNGFASAILAGLTRPVLALAGGLVLGVAESMVAGYAKASYQSSLALLLTLAILIAQAA
                     RREALHVVEE"
     gene            3757..4821
                     /locus_tag="VFQ35_22075"
     CDS             3757..4821
                     /locus_tag="VFQ35_22075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9933415.1"
                     /translation="MTRIDARSATLAAVACVALATAALLDESALSVYVLLALTASVTV
                     GVSLLMGHAGQVSLGHGAFYASGAYAAGILATHGFPSWLGLLVAPIAAASIAVVVGIP
                     LLLLRGHHLAFATLALHLIFLSIISELSITGGDVGLQGIPRLSLAGVALTSVRSYAYV
                     SWFALFLVVVVARNILRSRPGRALRALASSEVAAASSGVAVGYYKLAVFALSACFAGL
                     AGGIYAFYMGYLAPGSFPVMLSIEYIVMAAVGGLGKVSGALAGSAVVFLLVHGLSRVA
                     TSSGMPGSAPVILSYAVYAVLLVSAVLFLPGGLISFIGKAWARARRGFNAKDVPVASQ
                     GPARADSDPEGVPSNHGSFS"
     gene            4818..5846
                     /locus_tag="VFQ35_22080"
     CDS             4818..5846
                     /locus_tag="VFQ35_22080"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9933416.1"
                     /translation="MSSKIRVLSDPPESAPALAGETILESLLQAGLAFPHNCQSGNCG
                     ACKCELVEGDILELPYSEYALSAEERSRGLILACRTQVWGDCSVRPLGADEMVLHPSR
                     VMRCRVVEIQELTHDIRGLSLAIESGGPYGFSAGQHARLKFGPGIPERSYSMASLPGD
                     SMLEFYVRLTARGQASGYVFSNLRVGAEVTVSGPLGNAYLRENHRGPLLAVAGGSGMA
                     PIKSIVETALRNDPTRTVHCYFGVRDERDVYVESRLRELAQRHPNLRVHVVLSEASGA
                     TTRRTGTVATAVMEDFKEFPGFKAYVAGPPPMVEAVQRELVARGMAIRDIHADAFYSQ
                     ADDAFNLP"
     gene            5864..6802
                     /locus_tag="VFQ35_22085"
     CDS             5864..6802
                     /locus_tag="VFQ35_22085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9933417.1"
                     /translation="MSETAASGSLNGRVALVTGGARGIGLAISRQLHSLGASVVIADS
                     GVSMSGDTPDSAPATALAHELGERALPFTDDIATPDNAASAVSLAVSRFGALDIVVNN
                     AAILRDSLIFKGSAANWDAVLRTNLSAAFYVLAAATPHLREQAKSGRAPGSIVNITSS
                     AGLYGNFGQSGYASAKAGLLGLTRVVAMDMARSKVRCNAVAPFASTRVTEAIVPASTA
                     QAQYKERALKIPTRFVADFVAFLCGDEARDISGQLFGVRGREVFLFSQPRPIERFVVT
                     EPGAAGAEFTHLRAQFEAKFVDLTTDLEAFNTEPVT"
     gene            6857..>7173
                     /locus_tag="VFQ35_22090"
     CDS             6857..>7173
                     /locus_tag="VFQ35_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933418.1"
                     /translation="MANSYHEVKTVADLANEPPEYREAISKIVVSHTVNELYGAQVFD
                     EPAIALAPTPYAKWLSCRIAMEEYHHHVRFKGLADELGIPPAATDPSVKRPLSIFGFS
                     LKT"
BASE COUNT         1205 a         2366 c         2326 g         1276 t
ORIGIN      
        1 ggtaagctac aacgtcgcgg cttggttcgc ccgctttcag ctcggttatc gccggggtga
       61 tacgtacttg gcggcagccg gcatggcccc ggcgcggcgc caagagagca acatggctta
      121 tctaggaatc acgctcgcgg atccgtgagc gtttttcgag cgctacctgg tttgaatgag
      181 cccgatcgca cctaggacac tcgcgtcctc gtcgagaatt ggagaaatgt cgatggaatg
      241 cacccgccgc gcgctcgcaa ccgttgcgat tctattcttg gctgccgcat gccgaaaagg
      301 ctcggaaccg cagccagaga agcttcatcg ggagaccagc gccgccgcac cggcgaagtc
      361 ggagaccatt cgcatcgggc tcatcgcctc gttgaccggt aaatattccg cgctcggcag
      421 cgaagacaaa aaggcagtcg atctcgccgt cgagcagatc aaccaaagcg gagggttgtt
      481 gggcaaaaag ctcgaagtcc tgacgcgaga cgaccaaacg ctgcccgata aatcggtgct
      541 cgcctacaac gatctcaaag gacagaacgt ggtcgcggtc atcggctcgg tgttttcgaa
      601 ctccgcgttg gccacgctgc cgttggcgca aaacgatggg atgccgtacc tgtcactcac
      661 cccagccgaa gagcaggtca taccggtcaa gccgtacgtg ttcgtgatcc ccgcgttggc
      721 gaacgcttat gcggagcggt acctcgagta catgcaagcg cgcaaaatcg tgaagatcgc
      781 ggtggcccac gacacgaagg gcgcgtacgc catttctggc tacaacgcga tgaagacgct
      841 cgcgccgaag tacggcgtag agatagtgcg cgacgaggtg ttcgagacct cgaccaacga
      901 ctttagcccc atcttcacac acctcaaagg cgcgcccgcg caggcattcg tgttctgggg
      961 cactggacct tcgggggtca cggtcaccaa gcagtatgcg gcttcggggc tcaaagtgcc
     1021 gctcttcatg acgcccgccc aagcgagcaa actctggata gacccagtcg gcgcggctgg
     1081 cgaaggcgtg accgtcttga gcgcgattgg cgtggttgga gagtacctgc ccgacggacc
     1141 gcaaaagcag atcatccagc gcatggcggc gccgtaccag gaaaagcacg gctatccgcc
     1201 gccgcagttt gctcaggatg gctacagtgc gtgcctgctc ctgttcgagg cgatcaagaa
     1261 ggccgacagc acggagcgtc caaagatcca gcaggcgctc gagaccctca gcatggtgac
     1321 cccgaacgga gcgtacaagt acacgccgac ggatcactct ggcctgactc gtgactacat
     1381 ctcggtgaac gtcgtgcgcg agggcaagtt ggttccgacc gactgggcga aagagaagct
     1441 cgcgcgcacc gtcgccgagc agcactgaag gcgtcgatgc ttcacgtcga gggactcagc
     1501 cggcgattcg gcggcgtcta cgccgtgcag ggagtgagct tccgcgtcca gcccggcgag
     1561 cttcgaggcg tgatcgggcc gaacggcgcg ggcaagtcga cgctgttcca tctcatcgcc
     1621 gggcatctca agccgagcgc gggtcggatc accctcggga acgagcccat cgaccggctg
     1681 cgaccggatc agcgagcgcg ccgcggaatc tcgatcgtct accaagggga tcgcattttt
     1741 cggcgaatga gcgtgcgcga gaacgtcatg gtgggcgcgc actgcaaggc ccgccacggc
     1801 ttcttcgaag cggcgctacg tttgcctcgc tgccgccgag aagaccgaga gctgcacgat
     1861 aaagccgacg cggtgctcga gcgcgtcggc ctcgcggcgg tggcggagca accggcagag
     1921 tcgcttccgc tcggccagca gcgaacgatg caactcgcgc gcgccctcgc cgccgatccc
     1981 aaggtgttgc tcctcgacga gccggccgcg ggcctacgcg cgacggagcg aagccagctc
     2041 gctcgattga tacgcgaagt gcgcgcgcac gggctgaccg tgttgctggt cgaacacgac
     2101 gtggcgctcg taacgaccct ctccgacaca atcacggtcc tcgacctcgg tcgcgtcatc
     2161 gcagagggtg agccgagcgc tatccgccgc gaccccaagg tgctcgcggc ctacctcggg
     2221 gaggggccgc cgtcgtgatt caggtcgacg agttgagcgt gcgttacgga gccgcgctcg
     2281 cgctcgacgg cgtttcgctc aacgtcgccg cgggcgagaa ggtcgcgctg atcggcgcga
     2341 acggcgcagg caagtccacg ctgctgaacg cgctttctgg catggtaccc aagaccgccg
     2401 ggaacgtccg cttcggcggt cgcttcgccc acgtccccga gggcaggcag ctctttcccg
     2461 atctgagcgt cgaagacaac ctgcgactcg gagctttccg ggtgcgcgaa cgcaaccccg
     2521 cctggatcta cgagctttta cctgatctcg gtcggttgcg tcgacagcga gccggcagcc
     2581 tgtcaggcgg gcaacagcaa atggtcgcga tcggtcgcgg gctgatggcg cagccggacg
     2641 tcctcgcgat cgacgaaatg tcgctcggct tggcgcccat catcgttcag cagctcgccg
     2701 agcgcctttc cgagctcaac gccgcgcgag gtctcgcgct cttgctcgtc gagcagagcg
     2761 cccgcgtcgc tctttccctc tgcgagcgag cttacgtgct cgagacgggg cgcgtcgtcg
     2821 cacaagggtc ggcgagcgag ctcgcggatt cgccgttcgt cgtcagcgcg tatttgggca
     2881 gccccctcga agggtcgtcg tgagcgcctt cctctcctac ttcttccacg gcatctccgt
     2941 cggctgcggc ttcgcgctga tcgcgagcgg gctcatcatc atttaccggg tgacccgggt
     3001 cgtaaacctg gcgcaaggaa tgttcggcgt cgtcgccggc ttcagcgcga cgacgttgct
     3061 cgacctcggg ttgccccacg tgctcgcgga gctcgtcgcg atcgcggccg cggcgggagt
     3121 cggcgtggcc acgggctacg tcgcgatcgg aaaacgaggg acctcccctc aggcgtcgtt
     3181 gatcgcgacg ctcggcatcg gcgttttcgc gtacgccgtc gagattctca cttggggcga
     3241 ccagcctcgt tcgtttgccg ggtactcggg cgcgttcgag ctcgcgggcg tggaaatgcc
     3301 gaagcagtac gcgctcgtca ccgtcgtcac gctgctcgtc ttcggtgggc tcgaggcgtt
     3361 tttcgagcgc acctacgcgg gcaaggcgct gaccgcatgc gcgtcgaacc cctacgctgc
     3421 ggccctgcag ggcatcgagg ttcggcggat gggcctctac ggtttcgcgc tcgggggcgc
     3481 gctcggagga gtggcgggcg tgttgctgac gccgctccag ccggtctctt acgatagcga
     3541 cgtgttgctg ttcgtgaacg gcttcgcatc cgcgatcctc gccgggctga cgcgcccagt
     3601 gctcgcgctg gcgggcggcc tggtgctggg agtcgccgaa tccatggttg ccggctacgc
     3661 gaaagcttcg taccagagct cgcttgcgct gctcttgacg ctcgccatcc tcatcgcgca
     3721 ggcagcacga cgcgaggctc ttcacgtggt ggaagaatga ctcggatcga cgcgcgctct
     3781 gcaacgctcg ctgcggtcgc atgcgtcgcg ctcgcaaccg ccgcactgct cgacgagagc
     3841 gccctctccg tatacgtgct gctcgcgctc accgcgagcg tcaccgtcgg cgtctcgctg
     3901 ctcatgggcc acgcggggca ggtctcgctc gggcacggcg ccttctacgc ttcgggcgcg
     3961 tacgcggcgg gcatcctcgc gacccacggg ttcccgagct ggctggggtt gctggtcgcg
     4021 ccgatcgcgg cggcgtccat cgcggtggtc gtcggcatac cgttgctcct gctacgaggg
     4081 caccatctgg cgttcgccac gctggcgttg catctgattt tcctatcgat catcagcgag
     4141 ctttcgatca cgggcggcga cgtcgggctc caagggattc ctcgcctcag cctcgccggg
     4201 gtcgcgctga cctcggtccg gagctacgca tacgtgagct ggttcgcgct gttcctcgtc
     4261 gtcgtcgtgg ctcgcaacat tctccgttcg cggcccggac gcgcactgcg agcgttggcc
     4321 tcgagtgagg tcgccgccgc gtcatcgggc gtggccgtcg gctactacaa gctcgccgtg
     4381 ttcgcgttgt ccgcctgctt tgcgggcctc gcgggcggca tctacgcttt ttatatgggc
     4441 tacctcgctc cggggtcgtt cccggtcatg ctgtcgatcg agtacatcgt catggccgcg
     4501 gtcggcggtc tcgggaaggt ctccggggct ctggcaggca gcgccgtcgt gttcttgctc
     4561 gtgcacgggc tcagccgagt cgcgacgtcg agcgggatgc cgggctcggc gccggtcatc
     4621 ctgtcctacg ccgtttacgc ggtcctgctc gtcagcgccg tgctgtttct cccgggaggc
     4681 ttgatctcgt tcatcggcaa ggcttgggcg cgggctcgcc gcgggttcaa cgcgaaggac
     4741 gtacccgtcg cgtcgcaagg cccagcccgc gcggactccg accccgaagg cgtcccatcc
     4801 aaccacggga gcttctcttg agctccaaga tccgagtgct ttccgacccg cccgagagcg
     4861 ctccggcgct cgctggcgaa acgatcctcg agagcttgct tcaggccggc ctcgcgtttc
     4921 cgcacaactg tcagtccggt aactgcggag cgtgcaaatg cgaactggta gagggagaca
     4981 ttctcgaact cccttactcc gagtacgcgc tcagcgccga agagcgctct cgcgggttga
     5041 tcctcgcgtg tcgcactcag gtgtggggag attgcagcgt gcgcccgctc ggagcagacg
     5101 agatggtgct gcatccttcg cgcgtgatgc gttgccgagt cgtcgagatc caagagctga
     5161 ctcacgacat ccgcggcctc tccctcgcga tcgagagcgg cggtccgtac gggttttccg
     5221 cggggcagca cgctcgcctc aagttcggac caggcattcc cgagcgcagc tactcgatgg
     5281 cgagcttgcc gggcgactcg atgttggagt tttacgtccg actcacggcc cgaggacaag
     5341 cgagcggcta cgtcttcagt aacctgcgcg tcggggcgga ggtcacggtg tcgggaccgt
     5401 tggggaacgc ctacctgcga gagaaccatc gcggcccgct cttggcggta gcaggcggca
     5461 gtggcatggc cccaatcaaa tccatcgtcg agaccgcgct gcgcaacgac ccgactcgca
     5521 cggttcactg ctacttcggt gtgcgtgacg agcgagacgt gtacgtcgag agccgcctcc
     5581 gagagctcgc gcagcgtcac ccgaacttgc gggtccacgt ggtgctctcc gaagccagcg
     5641 gggcaaccac acgccgcacc gggaccgtcg ccacggcggt gatggaagac tttaaggagt
     5701 tccctggctt caaggcgtac gtcgccggtc cccctccgat ggtcgaggcg gtgcaacggg
     5761 agctcgtagc gagggggatg gcgattcgcg acatccacgc cgacgccttt tatagccagg
     5821 ccgacgacgc cttcaacttg ccctagcgaa gcgaggcaca gccatgagcg aaacagcagc
     5881 aagcgggtca ctgaatgggc gagtcgccct ggtcacgggc ggcgcgcgcg gaatcggcct
     5941 cgccatcagc cggcagctcc attcgctcgg cgcaagcgtc gtgatcgcgg acagcggcgt
     6001 ctcgatgagt ggtgacactc cggattcggc gccggccacc gctttggcgc atgaactcgg
     6061 cgagcgtgcg ctgccgttca ccgacgacat cgccacgccc gacaacgcgg cttcggccgt
     6121 gtcactcgcg gtcagccgct tcggcgcgct cgacatcgtc gtcaacaacg cggcgattct
     6181 gcgcgacagc ctcatcttca agggctcggc tgcaaactgg gatgcggtgc tgcgaaccaa
     6241 cctgagcgcg gcattctacg tgctcgccgc ggccacgccg catttacgcg agcaagcgaa
     6301 gagcggtcgt gccccgggca gcatcgtcaa catcacgtct tcggccgggc tctacggcaa
     6361 cttcggtcaa tccggctacg ccagcgccaa ggccggcctc ctcgggctga cccgcgtcgt
     6421 ggcgatggac atggctcgct ccaaagtcag gtgcaacgcg gtcgcgccct tcgccagcac
     6481 tcgcgtaacg gaagcgatag tcccggcgag cacggcgcaa gcgcagtaca aagagcgcgc
     6541 gctgaagatc cctacgcgct tcgtggccga cttcgtcgcg ttcctctgcg gagacgaggc
     6601 gcgcgacatc agcgggcaac tcttcggcgt gcgcggccga gaggtattct tgttctccca
     6661 gccccgtccg atcgagcggt tcgtcgtcac ggagccaggg gcggccggag ccgagttcac
     6721 tcacttgcga gcgcagttcg aagccaagtt cgtcgactta accaccgacc tcgaggcatt
     6781 caacaccgaa cccgtcactt aaggggagcc agcccgcgtc gaccaccact ctcaaccgac
     6841 cgccaggagg tctcaaatgg caaattcgta tcatgaagtg aagacagtcg ccgatctcgc
     6901 gaacgagccg ccggagtatc gcgaggcgat ctcgaagatc gtcgtgagcc acaccgtgaa
     6961 cgagctctac ggagcgcagg tcttcgacga gcccgccatc gccctcgcgc cgacgccata
     7021 tgccaaatgg ctgagctgcc gaatcgcgat ggaagagtat caccaccacg tgcgcttcaa
     7081 gggccttgcc gacgaactcg gcatcccgcc cgcggcgacc gacccgagcg tcaagcgacc
     7141 gctcagcatc tttggatttt cgctgaaaac ctg
//
LOCUS       DASTKS010001047         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1506578, whole genome shotgun sequence.
ACCESSION   DASTKS010001047 DASTKS010000000
VERSION     DASTKS010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506578"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..546
                     /locus_tag="VFQ35_22095"
     CDS             256..546
                     /locus_tag="VFQ35_22095"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET9933419.1"
                     /translation="MYTYVITRANGWANPEELKAAAAVSGRVGNEEMPDKVRWIRSYV
                     TRHEGGRLGTVCIYQATDPDAIREHARRVGMPADAITPIVDTVIVRPDPTNI"
     gene            642..1451
                     /locus_tag="VFQ35_22100"
     CDS             642..1451
                     /locus_tag="VFQ35_22100"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9933420.1"
                     /translation="MATRAPENFDAAELRGAIRLTYERVAREPRGEFHFHRGRAYAID
                     YLGYDAVELDAVPSIAVDRFAGVGNPLRIERIEPGQTVLDHACGAGLDLLLAARRVGD
                     TGRAIGVDITPAMREVAQRAAREANLGARVEVRAGNYEDLPVETNSVDVVISNGVVNL
                     APDKRRVLDEVFRVLRPGGRFFLADVVVARELKLETRQRPELWAACIAGALPERELLP
                     LTSEVGFVHQRTEGHFDCFAGTSAEHKVSRDLRVRSLNFFARKPSNPSSLG"
     gene            1606..2562
                     /locus_tag="VFQ35_22105"
     CDS             1606..2562
                     /locus_tag="VFQ35_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9933421.1"
                     /translation="MALESSVSLRGRVAVVAGATRGAGRGIAAALGEAGATVVCTGRT
                     TRFARSEYDRPETIEETAELVTSLGGRGIAVPVDHLDHAQVQALAARIRGEFGHVDIL
                     VNDIWGAELLKGMPPDWNKPIWEHDIEKGLRILRLAIDTHLITSHYLLPLVVSRPGGL
                     LVEVTDGTNEYNGAHYRISVFYDLAKVAVNRLAYSQGHELASYAATAVAVTPGWMRSE
                     IMLEAFGVAEADWRSSLEAREGKPSAPPDFALSESPRYVGRGIAALAADAGRARWNQR
                     SVTAGQLAAEYGVTDIDGSRPDVWRYMADVEANRAEPLASYR"
     gene            2757..3317
                     /locus_tag="VFQ35_22110"
     CDS             2757..3317
                     /locus_tag="VFQ35_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933422.1"
                     /translation="MKQGMLMMVLAGLSLSVPAFAEAPKAPTQLRPPSACQKNPALCK
                     NVVANVACPDPGAQIEFRLVRRTSQFAGVIEVKASVKNFGGPYVTGPNQQLLNLYEVV
                     PGAAPRLVASKPFANLASGEVASLTFSRTWNASSPAEGEFPPSYRAIISYDPDILLDG
                     NVKNDDCRAANNVIEQSGTQINALFH"
     gene            complement(3502..>3730)
                     /locus_tag="VFQ35_22115"
     CDS             complement(3502..>3730)
                     /locus_tag="VFQ35_22115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9933423.1"
                     /translation="LACAEGQGIALLPSYVEAHEARLQRLLPRLVCPSRALYSVTHAD
                     LRESSRVRAFQSFAEDALTRANEGSATAREI"
BASE COUNT          687 a         1150 c         1226 g          667 t
ORIGIN      
        1 cacgagcagg tcgaagacga ggggtggcag cacttgttcg tgtccgtcga ggcggagggc
       61 tcgcccctgc tcgtcgagct cgaagggacc gaacgagaag tgcatgacct tggcgttagc
      121 gaagggtgga ggctggggca atcgataagg gtttggtcag gctgcggaaa ggactttctc
      181 ggccaagtac cgcaatttca gcccacttcg gaacgcgacg tttcgaccaa gcacccgaga
      241 aacgaaggag acaccatgta cacatacgtc atcactcgcg cgaacggttg ggccaacccg
      301 gaagagctga aggcagcggc tgcggtttcc ggccgtgtgg gtaacgaaga aatgccggac
      361 aaggttcgct ggatccgcag ctacgtgacg cgtcacgagg gtggacggct cgggaccgtc
      421 tgcatttatc aagcgacgga tcccgatgcg atccgcgagc acgctcgccg cgtcggcatg
      481 cccgcagacg ccattacgcc catcgtcgac accgtcatcg tgaggccaga tcctacgaac
      541 atctgagcgt cggcgtcgga tcgtgctccg cgcgaggcaa cgatcgcgtt gctttcggcg
      601 gagcccggtc cggcatctca aagcgaaagg accagcgaaa catggcaacc agagccccag
      661 aaaacttcga cgcggcggag ctccgcgggg cgatccgtct cacttacgag cgtgttgctc
      721 gcgagccccg cggcgagttt cactttcatc gcggtcgcgc ctacgccatc gactaccttg
      781 gctacgatgc ggtggagctc gacgcggtgc ccagcattgc ggtcgaccgc tttgcagggg
      841 ttggaaaccc gctgcgcatc gagcggatcg agccgggcca gacggtgctc gatcatgctt
      901 gcggcgccgg gctcgatctg ctgctcgccg cgcgccgcgt gggagatacg ggacgcgcca
      961 tcggcgtcga catcacgccg gccatgcgtg aggtggccca acgtgccgcg cgcgaagcga
     1021 acctaggagc tcgcgtcgaa gttcgcgcgg gaaactacga agatttgccg gtcgaaacca
     1081 acagcgtcga cgtggtgata tcgaacggcg tcgtgaacct cgctccggac aagcgccgcg
     1141 tgctcgacga ggtatttcgc gtgttgaggc cgggtgggcg tttctttctc gctgacgtgg
     1201 tcgtcgctcg cgagctcaag ctcgaaacgc gccagcgccc cgaactatgg gcggcgtgca
     1261 tcgcgggcgc gttgccagag cgcgaactgc tcccgctcac gagtgaagtc ggcttcgtcc
     1321 accagcggac ggaaggccat ttcgactgct tcgcgggtac gagcgcggag cacaaagtga
     1381 gccgcgactt acgcgtccgg agcctgaact ttttcgcgcg gaagccctca aacccaagct
     1441 cgcttgggta gatggaaccg cgtcagacac cgagtgacag atctggccaa aggactgcga
     1501 gatttgcgat cctcgcctaa cgtggaccgc tagccttgcg gtccgttcga gcggcagcgg
     1561 taacccttcg ttgccttggc caaggcggca aactaaagct gcggcatggc tttggagagc
     1621 tcggtttcgt tgcgcgggcg cgtcgcagtg gtagcgggag caacacgcgg ggctggtcgc
     1681 ggcatcgcgg ctgcgttggg cgaggccggc gccacggtgg tctgcactgg tcgaacgacg
     1741 cgcttcgcgc gctcggagta cgatcgtccc gaaacgatcg aagaaacggc cgagctcgtt
     1801 acgagcctgg gcggccgcgg aatcgcggtg ccggtagatc acctcgatca cgcgcaggta
     1861 caggcgctcg cggcgcgcat tcgaggcgag ttcggccatg tcgacatttt ggtgaatgac
     1921 atctggggtg ccgagcttct gaaggggatg cctcccgact ggaacaaacc gatctgggag
     1981 cacgacatcg agaagggttt gcgcatcctc cggctcgcga tcgatacgca cttgattacg
     2041 tcacactacc tcttgccgtt ggtggttagc cgccccggtg gcttgctcgt cgaggtgacg
     2101 gatggaacga acgagtacaa cggcgcgcac taccgaatct cggtgtttta cgatctcgcg
     2161 aaggtggccg tgaaccggct cgcgtattcg caaggacacg agcttgcgtc gtacgcggcg
     2221 acggcggtcg cggtcacgcc tggatggatg cgctctgaaa tcatgctcga agcgttcggt
     2281 gttgcggagg cagattggcg gagctcgctc gaggcgcgtg agggcaagcc ctcggcgcca
     2341 cccgatttcg cgctgtcaga gtcccctcgt tacgtcgggc gcggaatcgc cgcgctcgcc
     2401 gcggacgcgg gtcgggcgcg ctggaaccaa cgatcggtca ctgcgggtca gctcgcagcc
     2461 gagtacggcg tcaccgatat cgacggatca cgccccgacg tttggcgcta catggccgac
     2521 gtcgaggcga atcgagctga gcccctcgcg agctatcgtt agtgtcgcga gttcgcttcc
     2581 tttgcggctc gacttcgcgt cgaaagagcc tcttgtaatt gcgcgcaatt cgccgcaatc
     2641 ccctgaaatc gccgcgaata acgcgaggtt cgggattcga agacgggaag caaacatgcc
     2701 ggtctccgta atctaatcat cggcgcatcg aacgacgccg acggaggata cgcgagatga
     2761 aacaaggcat gttgatgatg gttctggcgg gtctgtctct ttcggtgccg gcgtttgcgg
     2821 aggcccccaa ggcccccacg cagctcaggc cgccctctgc gtgtcagaag aatcccgctc
     2881 tctgcaagaa cgtcgtcgcc aacgtggctt gcccggaccc cggggcgcag atcgagtttc
     2941 ggctcgtgcg gcgcacgtcc caatttgccg gcgtgatcga ggtgaaggct tccgtcaaga
     3001 acttcggcgg accctacgtc acgggcccca atcagcagct cctgaacctc tacgaggtcg
     3061 tccccggcgc cgcgccgcgc ctcgtcgctt cgaagccgtt cgcgaacttg gcgtccggcg
     3121 aggtcgcctc gctcaccttc agccgcacgt ggaatgcctc gtcgcccgcg gagggcgagt
     3181 ttccgcccag ctatcgcgcc atcatttcgt acgacccgga tatcctcctc gacggcaacg
     3241 tcaagaatga cgactgtcgc gccgcgaaca acgtgatcga gcagagcgga acacagatca
     3301 acgcgctctt tcactgaaca tcgtgagggc gcgcgtccac gactgagcgc aaagccgcga
     3361 gcttcgcccc tggggtgctc gcggtaatgg cgctcggttc gttcgaacgg cgcgtcaaag
     3421 cggctgtttt gatccccgtc acgaagaacg tcaccctaga ggtccggatg tggacgttcc
     3481 gaggagattg cctgcactct cctagatttc gcgcgctgtt gcagagcctt cgttcgcgcg
     3541 ggtcaaagcg tcctcggcga acgactgaaa tgcgcggacg cgactgcttt cacgcaggtc
     3601 ggcgtgcgtt acggagtaga gcgcccgcga agggcaaacg agccgtggca gcaaacgctg
     3661 taggcgcgcc tcgtgtgcct ccacgtagct cggcaagagg gcgatgccct gcccttcggc
     3721 gcacgcgagg
//
LOCUS       DASTKS010001048         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_387919, whole genome shotgun sequence.
ACCESSION   DASTKS010001048 DASTKS010000000
VERSION     DASTKS010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387919"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1245)
                     /locus_tag="VFQ35_22120"
     CDS             complement(118..1245)
                     /locus_tag="VFQ35_22120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0565 family glycosyltransferase"
                     /protein_id="HET9933424.1"
                     /translation="MKLSIGIFTYSTRPRGSVVHACALAEALTDAGHDVTLYALSKAN
                     DDWYRSLRCRSVLIPAEAAPADADALIAQRVEELRRGLRSLAPRHDVYHAEDCLAASG
                     LLAAGALRPLVRTVHHVEHYDSAYLRQCQRRSVLEADGVLCVSKASQRDVLGEFGVRA
                     TVVPNGVELERFDARLASRERLVLSVGGVEPRKNSVGMLRAMSLVLDRVPDAHWVIVG
                     GASIWEHAEYRARFAAELAALPDGIRARVHQLGTLSEEELATVYARSELMLHASVQEG
                     FGLCVLEALAARVPVVVSRGEPFDEYLDESCASFVDPTSPSSIAAGVLDLLRDRPLMR
                     ARKSAGRVRAESFSWAAAARLHVAFYTSLVSATSERSLSHA"
     gene            complement(1242..2228)
                     /locus_tag="VFQ35_22125"
     CDS             complement(1242..2228)
                     /locus_tag="VFQ35_22125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HET9933425.1"
                     /translation="MKLGGYSLSTLCEALRARPELELKRDIRLPARVLGARSESAWFA
                     GGDIANGDDTAALPDGDGFTLFAAEGMRPEFVAADPWFAGFCAVMVNLSDVAAMGGRP
                     WAVVDVLFSGSSDNEQILLGMREASRSFGVPIVGGHTTRVDGNSVLAVAVLGRARRLI
                     SSYRARPGQAILAAFDLRGAFRGATSQFDAATSASAATLRRNLSVLPELAEAELVSAG
                     KDVSMAGLCGSLAMLLETSGVGARLTLDEVPAPPNVDALRWLTAFPSFGFVLTAEPER
                     AEEVQRRFAAVGLTCSVVGEIDASRRLELCSFGERALYADLGVEVLTGFGSR"
     gene            complement(2225..2776)
                     /locus_tag="VFQ35_22130"
     CDS             complement(2225..2776)
                     /locus_tag="VFQ35_22130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HET9933426.1"
                     /translation="MLDAVNPWGRPIRPSLGAEIVAELATEPWQVREYRDLRRSIFAE
                     EQGLFEGSDTDAHDAYALPIVAMSQIAGMADRIVGVVRIYASEKDTWYGGRLGVRREY
                     RRFGAVGTALIEAAVSTAHFLGCRRFLATVQEQNVRYFERHHFAVREELSVCGRSHRL
                     MEADLAAYPPRAIGHRFVQARVA"
     gene            complement(2769..>3091)
                     /locus_tag="VFQ35_22135"
     CDS             complement(2769..>3091)
                     /locus_tag="VFQ35_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9933427.1"
                     /translation="HALGVFGRGQVSTYILAGLGDTREEILEACERLAGIGVYPFVVP
                     FVPIAGTPLENQPPPDPAFMSALLSDVSRLLARHALSSAEIKAGCGRCGACSSLRVRE
                     AVHA"
BASE COUNT          591 a         1077 c          972 g          451 t
ORIGIN      
        1 tcgacgaagg ctgcgacagg atagctttgt cctgcttcga ggtgctcctc gatcacgtaa
       61 gaaggcgaat agcagtcttg gactaggcca tgcggccagc gaacgcgaaa attcatctca
      121 ggcatgggac agactccgct ccgaagtcgc gctcacgagc gacgtgtaga aagcgacgtg
      181 caaacgggcg gcggccgccc acgaaaaact ctcggcgcgc acgcgaccag cggatttgcg
      241 ggcgcgcatc agcggccggt ctcggagcaa gtcgaggacg cccgccgcga tcgacgaagg
      301 cgaagtcggg tcgacgaaac tcgcacagct ctcgtccagg tactcgtcga acggctcacc
      361 gcgagaaacg acgaccggaa cgcgggcggc gagtgcttcg agcacgcaaa ggccaaaccc
      421 ctcttgaacc gaggcgtgaa gcatgagctc gcttcgcgca tatacggtcg cgagctcttc
      481 ttcactcagg gtgccgagct gatgcacacg cgcgcgaatt ccatctggca gtgccgcgag
      541 ctccgccgca aaccgcgcac ggtactcggc gtgctcccaa atgctcgcgc cgccgacgat
      601 tacccagtgc gcgtcgggca cgcgatcgag caccaacgac atcgcgcgca gcataccgac
      661 gctgttcttg cgcggctcga cgcccccgac gctgagcacc aggcgctctc tcgaggcaag
      721 ccgcgcatcg aagcgctcga gctcgactcc gttcggcact acggttgcgc gcacgccgaa
      781 ctcgcccagc acgtcacgct gacttgcctt gctgacgcag agcacgccat ccgcttccaa
      841 gacagagcga cgctggcatt gtcgcagata ggcgctgtca taatgctcta catggtgaac
      901 ggtgcgtacc agcgggcgga gagccccagc ggccaacaag ccgctcgcgg cgaggcagtc
      961 ctcggcgtgg taaacgtcgt gacgcggcgc caggctacgt aacccccgcc ggagctcttc
     1021 gactcgctgc gcgatcaaag cgtcggcgtc ggcgggcgcg gcctcggccg gaatcaggac
     1081 gcttcggcaa cggagcgagc ggtaccagtc gtcgttggcc ttcgacaaag cgtagagcgt
     1141 aacgtcgtgc cctgcgtcgg tgagcgcctc ggcgagcgca caggcgtgca cgacgctacc
     1201 ccgcgggcgc gtcgagtagg tgaagatgcc aatcgagagc ttcatcgcga gccaaaaccg
     1261 gtgaggactt ccacgcccaa gtccgcgtac agcgctcgtt cgccgaaaga gcagagctcg
     1321 agccgccgcg aggcgtcgat ttcaccgacc acgctgcacg tcagcccgac agccgcgaac
     1381 cgtcgctgga cttcctcggc tcgctcgggc tccgcggtca acacgaaccc gaagcttgga
     1441 aacgcagtca accaacgcaa cgcatcgacg ttcggcggcg ccggaacctc gtcgagcgtg
     1501 aggcgcgctc ccacccccga agtctcgagc aacatcgcga gcgagccgca gaggccagcc
     1561 atgctcacgt ccttgcccgc cgaaaccaac tccgcctccg caagctccgg caacacgctc
     1621 aagttgcgcc gaagtgtcgc ggcgctcgcc gacgtcgcgg catcgaactg actcgtcgcg
     1681 ccacgaaagg ctccccgcag gtcgaacgcc gccaagatgg cttgacccgg ccgagctcga
     1741 tagctcgaga tgaggcggcg cgcgcgcccc agcaccgcga ccgcgagcac gctgtttccg
     1801 tcgacgcgcg tcgtgtgtcc gcccacgatc ggcaccccga acgagcgaga cgcctcacgc
     1861 atcccgagca gtatttgctc gttgtcggag cttccggaga agagcacgtc gacgacggcc
     1921 caggggcgtc ctcccatcgc cgcgacgtcg ctcaaattca ccatcaccgc gcagaaaccg
     1981 gcaaaccagg ggtcggcggc gacgaactcg gggcgcatgc cttcggccgc gaacagcgtg
     2041 aacccgtcgc cgtccgggag cgcggcggtg tcgtcgccgt tcgcgatgtc gccaccagca
     2101 aaccaggcac tctcgctgcg agcgccgagc acgcgggccg gaagccggat gtcgcgcttg
     2161 agctccaact ctgggcgcgc tcgcagtgcc tcgcagagcg tcgagagcga gtagccgccg
     2221 agcttcacgc taccctcgct tgaacgaagc ggtgaccgat tgcgcgcggg ggatacgcgg
     2281 cgagatctgc ttccatcagt cggtgagacc gaccacaaac cgagagttct tcgcgcaccg
     2341 cgaagtggtg ccgctcgaag tagcgcacgt tctgctcttg caccgttgcc aagaaccgcc
     2401 tgcagccgag aaagtgcgcc gtactcaccg cggcttcgat gagcgccgtg ccgaccgcgc
     2461 cgaaccggcg gtactcgcgt cgcacgccga gacgcccgcc gtaccaggta tccttctcgc
     2521 tcgcgtaaat ccgaacgacg cccacgattc ggtcggccat ccccgcgatc tggctcatcg
     2581 ctacgatggg cagtgcgtag gcgtcgtgcg cgtcggtgtc ggacccctcg aacaatccct
     2641 gctcttccgc gaaaatcgag cgccgcaagt cgcggtattc acgaacctgc catggctcgg
     2701 tcgcgagctc cgcgacgatc tccgcgccga ggctcgggcg gatcggcctg ccccacgggt
     2761 tcacggcgtc aagcatgaac ggcctcccgc acccggagcg aagagcatgc cccgcaacgg
     2821 ccgcagcccg ctttgatttc cgcgctcgag agcgcatgtc gcgcgagcaa tcgcgacacg
     2881 tcgctcagca aagccgacat gaacgcggga tccggcgggg gttggttttc gagaggcgtt
     2941 ccggcgatag gaacgaacgg aactacgaac gggtacacgc cgatgccggc gaggcgttca
     3001 caagcttcca agatctcttc gcgcgtgtcg ccgagccccg cgaggatgta cgtagatacc
     3061 tgcccacgcc cgaagacgcc gagcgcgtgc g
//
LOCUS       DASTKS010001049         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1953403, whole genome shotgun sequence.
ACCESSION   DASTKS010001049 DASTKS010000000
VERSION     DASTKS010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953403"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..575
                     /locus_tag="VFQ35_22140"
     CDS             309..575
                     /locus_tag="VFQ35_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933428.1"
                     /translation="MNALISEAQARLHHVLGLSPEASARAVAEVLDSFRFDVDEYIAE
                     RHTQLQSAGIQNHEIYERIAAELPSLRFRAPLLTARQIRRRIYG"
     gene            598..2427
                     /gene="glmS"
                     /locus_tag="VFQ35_22145"
     CDS             598..2427
                     /gene="glmS"
                     /locus_tag="VFQ35_22145"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9933429.1"
                     /translation="MCGIMGYIGSQEAWPIVVGGLRRLEYRGYDSAGIVTVSRKRFRL
                     ARKVGHLRALEEATPEGLPGNVGIGHTRWATHGGVTEANCHPHLDSKERIALVHNGII
                     DNVEALRAELAASGTQFRSETDTEVLAELVGRGVESGLALRDAVLAAMKRIEGTAGIV
                     VVDRLEPEVLVAARLGSPVVVGLGEGSSWVASDALALRPYTDRMIVLDDGEIAELTPN
                     SIRTIDLEQRDRDKRVEKILHQPEDADVGDYPHFMLKEIYEQPNALDRSMRGRLDAAV
                     GSVRLGGLQTHESRLFDIRRVVFFACGTSLYSAQVGAYLMSRYARVPATAEDAAELSV
                     QNPIVDRHTLYVAISQSGETADTLVALREIRLRGGLIAGITNVVGSSLSRETDFGVYV
                     HAGPEISVCSTKAFSAQVLAAELIALRFARMRDLSANDGRLWVEALERLPQQLRLMLE
                     QAEACKELAHKFADARFTMFVGRGVNVPVAGEGALKLKEIAYLPAEGLSGAGMKHGPL
                     ALIEPGGPVWALVPPDETRERMIGNLRELKARGAYIMAVADAKDTEVAQIADRVIQLP
                     PHHPTVSPILNVVPLQLYAYYVALAKGYDIDKPRNLAKSVTVT"
BASE COUNT          420 a          827 c          770 g          424 t
ORIGIN      
        1 cctcgaaagg cttcagcctt cctccggtgc tcactcctcg ggcgcgcttc tcgctcgcgt
       61 ttcttggctc tcgcgacggg caggaacttg ggacaccccc tagttcgcat cgaatgtccc
      121 gccttcgcag cacgatggcc gagctacggc gctgcttctc tcgcgaccca acggccgagt
      181 cacgcttcgt gacgtggggt gagatctgtc ccgctagtaa caagtttgcc ccaataaccc
      241 ctcgaagcgt gcccggaaac gggcagccca ctcgacttcg gcgcccaaca ctgccagatt
      301 agccagccgt gaacgcgctg atatctgaag cccaggcgcg gctgcatcat gtcctcggtc
      361 tctcacccga ggcgtcggct cgcgcagtcg ctgaggtttt ggacagcttc cgcttcgacg
      421 tcgacgagta catcgcggag cgacacacgc agcttcagag cgcgggcatt cagaaccacg
      481 agatttacga gcgcatcgca gcggagctac cgtcgctgag gttccgagct ccgttgctaa
      541 ctgcccgcca aatccggcgg cgtatctacg gctgaacttc gacaaacgag ggcgaacatg
      601 tgcggcatca tgggatacat tggctcacaa gaagcttggc cgatcgtcgt cggcggtctg
      661 cgccggctcg agtaccgtgg ctatgactcc gcggggatcg tcacggtgtc gcgcaagcga
      721 ttccggctgg cgaggaaggt cggccatctc cgagcgctcg aagaggcaac gccggaaggg
      781 cttcccggca acgttggcat tggtcacacg cgctgggcga cgcacggcgg cgtcaccgaa
      841 gcgaactgcc atcctcacct cgactcgaag gagcgcatcg cgctcgttca caacggcatc
      901 atcgacaacg tcgaggcgtt gcgcgccgag ctcgcggcat cgggcacgca gtttcgctcc
      961 gagacggaca ccgaggtgct agcggagctg gtcggccgcg gcgtcgagtc cggtttggcg
     1021 ctgcgcgacg cggtgctcgc cgcgatgaag cgcatcgaag gcacggccgg catcgtcgtc
     1081 gtcgatcggc tcgaacccga ggtgctcgtt gcagcgcgtc tcggcagccc cgtggtcgtc
     1141 ggtctcggcg aaggctcgtc ttgggtcgcc tccgatgcct tggcgctgcg cccctacacc
     1201 gatcgcatga tcgtgctcga cgacggcgag atcgcggagc tcaccccgaa ctcgatccgc
     1261 accatcgacc tcgagcagcg cgaccgcgac aagcgcgtcg agaagatttt gcaccagccc
     1321 gaagacgcgg acgtcggcga ctacccgcac ttcatgctca aggaaatcta cgagcagccg
     1381 aacgcgctcg atcggtcgat gcgcggccgt ctcgacgcgg cggtcggctc ggtgcgcctc
     1441 ggcgggcttc agacccacga gtcccggctg ttcgacatcc gccgggtcgt ctttttcgcg
     1501 tgcggcacga gcctctacag cgcgcaggtc ggcgcctatt tgatgagccg ctatgcgcgc
     1561 gtgccggcaa ccgccgagga cgccgccgag ctttcggttc aaaacccaat cgtcgaccgg
     1621 cacacgctct acgtcgccat ttcacagagc ggcgaaaccg ccgacacgct ggtcgccttg
     1681 cgggagatcc gcctgcgcgg cgggttgatc gccggcatca ccaacgtcgt cggtagctcc
     1741 ctctcgcggg aaaccgactt tggcgtgtac gtgcacgccg gacccgaaat cagcgtttgc
     1801 tccaccaagg cgttcagcgc tcaggtgctc gccgccgagc tcatcgctct ccggttcgcc
     1861 cgcatgcgcg acctcagcgc gaacgacggc cgcctctggg tcgaagcttt ggagcgtctg
     1921 ccgcagcagc tccggctcat gctcgagcag gcggaagctt gcaaggagct cgcgcacaag
     1981 ttcgccgacg ctcgcttcac gatgttcgtc ggtcgcggcg tgaacgtgcc ggtggccggt
     2041 gagggcgccc tcaagttgaa ggagatcgcg tacctacccg ccgaagggct ctcgggtgcg
     2101 ggcatgaagc acggcccctt ggcgctcatc gagccgggcg gcccggtttg ggccctggtt
     2161 ccccccgacg agacgcgaga gcggatgatc ggcaatctgc gcgagctcaa ggcgcgcggg
     2221 gcctacatca tggcggtggc ggacgccaag gacaccgagg tcgcccaaat cgcggatcgg
     2281 gtgattcagc tacccccgca ccaccccacc gtctccccga ttttgaacgt cgtcccgcta
     2341 caactttacg cctactacgt ggccctcgcg aagggttacg acatcgataa gccgcgcaat
     2401 ctggcaaagt cggtgacggt gacgtaagaa gctcttccga g
//
LOCUS       DASTKS010001050         8321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_835146, whole genome shotgun sequence.
ACCESSION   DASTKS010001050 DASTKS010000000
VERSION     DASTKS010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835146"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFQ35_22150"
     CDS             <1..266
                     /locus_tag="VFQ35_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933430.1"
                     /translation="TYCVQRIRKAEIKARIDATELTDGTLQTACEQACPTAAIVFGDL
                     ADPSSRVSRLHTNQRAFEVLNELGTRPRTRYLAKLRNPNPEVT"
     gene            263..1681
                     /gene="nrfD"
                     /locus_tag="VFQ35_22155"
     CDS             263..1681
                     /gene="nrfD"
                     /locus_tag="VFQ35_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9933431.1"
                     /translation="MTDLDAAFWSSGSSTSARLEPPRPEPPERDRALLTTRVGDGMLT
                     EELFAKTLNPPAWLRPALWVTGLGSTLLVASVAYTLLTGIGVWGNNIPVGWAFAITNF
                     VWWIGIGHAGTFISAFVLLLEQKWRTSINRFAEAMTLFALVQAGLFPLLHLGRSWFFY
                     WLIPYPSTMGVWPQFRSSLTWDVVAITTYLTVSLLFWYLGLVPDLAALRDHVRDRWLK
                     RVYAVFALGFRGSAENWRRHRVAYGLIGGLAAPLVVSVHSIVSMDFAIGVLPGWHSTI
                     FPPYFVAGAIFSGFAMVLTLLIPARRLYRLEQVVTREHLDKMAKLVLVTSMIVTYAYA
                     VETYCAWLGGDTFDAHMHLVVRPKGTYAPLFWLQICCNCLVPQLLWSRRARRSPVVLF
                     ALSIFIQIGMWTERFVLIVTSQSEDFLPSSYRSYAPSLVDAAVLGGTLVFFAFLFLLF
                     LRFVPFIPVSELKALKHELGDS"
     gene            1678..2223
                     /locus_tag="VFQ35_22160"
     CDS             1678..2223
                     /locus_tag="VFQ35_22160"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET9933432.1"
                     /translation="MMRKKTPQLFAEFANADHALRAAEELRRRHLRLTSAYSPFEIPG
                     LERYVHTSRPFMIPLLSLLAALGGMALAYSLIWWTAAFDYPLNVGGRPLNSFPADIPI
                     LFETGILCAAVCAFICVLFFSGLPRLEHELEEIPGFERTSIDRYWLGIAATGSLPDAE
                     LEALLTRVGALHVHRLDGGRT"
     gene            2220..2846
                     /locus_tag="VFQ35_22165"
     CDS             2220..2846
                     /locus_tag="VFQ35_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9933433.1"
                     /translation="MISLANEHGLVAALFATLLFGCDNRQAFRRPEPGLERMLTQPRS
                     KPYRASTLFGDGRVMRTPPRGSVSREAVHSGDLLVTEGRTAQGYATRVPLRLTTEVLQ
                     RGRTAFENVCATCHGVLGTGVSFVAEKMELRKPPSLFEPRIVALPPGRVFEIVSRGYG
                     LMPSFDALLGIDARWAVVGYLDVLRASRAIKLAELPPDLQRRFAEVTP"
     gene            2843..3991
                     /locus_tag="VFQ35_22170"
     CDS             2843..3991
                     /locus_tag="VFQ35_22170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933434.1"
                     /translation="MRRGVIALGALGALGVIGCGAQASLHVGVGAPGYLAAFAFLTST
                     VLGGSLLLMVAELSSASWFVVLRRLAEALAGVTPLVAVAFVPLALALPALYPWARSFD
                     GLDETLRRSLEHARGWMNPPLFLGRSALIIVLWIALAEGLRRASLGEDAGQGEGSKRR
                     LTRLSALGVPLVAFTGSLAIFDWFMSALPGFTSNILGLYVLCGGFASAMGVVCVAFHV
                     ARKRALFPSRVAHYHTHALGRMLLMSVCLWAYIAVSQLVIVWSANLPKEAGFYLARYR
                     GPFRALAIGLVFAHFLLPFLLLLSRWLKQRSLALALLGGLIVLMHAVDMYWLLAPVSP
                     RAASFVDCAPFLLLGAVAASFGLWRFSRAPRVPTQAAELERSLAYESP"
     gene            3988..4326
                     /locus_tag="VFQ35_22175"
     CDS             3988..4326
                     /locus_tag="VFQ35_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933435.1"
                     /translation="MTSRFRTIQENDSVPLGRSLTVVLFSVLVGGAAVGASVLLSGAR
                     IPKPSFAPPSSAPTSMPEQSLIERTARGAHLHSAQRAALERYAWVDRDAGIVSIPIDR
                     AIELKAEKEP"
     gene            4326..5381
                     /locus_tag="VFQ35_22180"
     CDS             4326..5381
                     /locus_tag="VFQ35_22180"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9933436.1"
                     /translation="MLELFRRLFALPRGASSVAAGVDWLHFLVIGTTLLGALGIGVLA
                     TALIIRNRNEARGGATPRIAASRREEALVIAGILGLFCAFWLIGFRQYLEMQRPPSGA
                     RRVYVTAKQWMWKFTDIEGRSSNDVLTVEVGAKVELLMTSRDVIHSFFVPAFRLKRDV
                     VPGRYTAIWFQPVETGEFPIYCAEYCGVAHSNMAGVVRVLSSADYGRWLQGAEPSKGQ
                     SLAEVGREAAVRHACFACHTVDGQPHIGPTWSGLYGSTVELADGHRVIADAAYLTRSM
                     MEPSAELVAGYPPVMPTYLGSVSQPETAALIEYIRSLQHTNPAPSVTLPRLVSETARA
                     ADAPSNPAPRGGPERAP"
     gene            5378..7006
                     /locus_tag="VFQ35_22185"
     CDS             5378..7006
                     /locus_tag="VFQ35_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9933437.1"
                     /translation="MTLVSEIANAPREPNYVGAKTSVRSWLLSTDHKRIGILYFVATS
                     LALSLGGVFALLLRIEHLTPERTIVDAITYNRLFTLHGITMVWLFMIPSIPAAFGNFL
                     LPLQLGASEVAFPRLNLLSFWIYVVGAAFVLAALWLGGVDTGWTFYAPYSSKAPSAVV
                     PAVIGIFIVGWSTILTGVNFIVTVHTMRCAGIGWMRMPLFTWAMYGTSIIQVLATPVL
                     GLSLLIVLVDHLFSWGLFDPARGGDPVLYQHLFWFYSHPAVYIMILPAMGVVSEVVST
                     FAHKNPHSYRAIVYSTLGIAFVGFLTWGHHMFVAGMSTFDAGAFGVLSMLVAIFSAIK
                     VFAWVSTLRQGAIWLRTPLLYVIGFLFLFVFGGMSGVAVATMSLDVHWHDTYFVVAHF
                     HFVMVGATLTAWLAAIHYWFPKVTGKTYSETWGTVGALLVCVGLVATFFPQFLLGNAG
                     MPRRYFNYDPRFQVLNVASTLGSWVLATGMLVALAVIVHALFRGRPAGDNPWLSRCFE
                     WRTSSPPPEKNFAREPELSVDAYDYTTPWPEQPS"
     gene            7003..7632
                     /locus_tag="VFQ35_22190"
     CDS             7003..7632
                     /locus_tag="VFQ35_22190"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9933438.1"
                     /translation="MNSVHAEHFESAPKQAHAARLGMWIFLASELLLFAGLFALYGGY
                     RAHWPAAFREGIRHSTKALGSLNTGILLVSSTFAALSVHATRAGRRSRSLLLLAGTFL
                     LGLLFLGIKLYEYSLHFREGIYPGGSGAFFSAHPDPGLVPFWTLYFATTGLHAIHVSV
                     GLIVLGCTCFALWRGTVGVHRAHPLENAALYWHLIDTIWIFVWPLYYLA"
     gene            7637..7930
                     /locus_tag="VFQ35_22195"
     CDS             7637..7930
                     /locus_tag="VFQ35_22195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET9933439.1"
                     /translation="MPTKHHSERRSAIAPLAAGALLLILAVASYGLSFVSLGFWALPI
                     ALSIAALKACIVVSIFMELRRETASIQLTALMAALLLLILVALTAADVATRGR"
BASE COUNT         1238 a         2772 c         2536 g         1775 t
ORIGIN      
        1 gcacgtactg cgtgcagcgc atccgcaagg ccgaaatcaa ggcgcgaatc gacgctacgg
       61 aactcacgga tggcaccttg caaaccgcgt gtgaacaggc gtgccccacg gccgccatcg
      121 tgtttggaga tctcgccgat ccatcgagca gggtatcgcg cctccacacg aaccagcgcg
      181 ccttcgaggt cctgaacgag ctcgggacga gaccgcgcac gcgctacctc gccaagctcc
      241 gcaacccgaa ccccgaggtc acatgaccga cctcgacgcc gcattttgga gctcaggctc
      301 gagcaccagc gcccgactgg agccgccgcg accggagccg cctgagcgtg accgtgcgct
      361 gctcaccacg cgcgtcggcg atggcatgct gaccgaagag ctcttcgcga agacgctgaa
      421 cccgcccgcc tggcttcgtc cggcgctgtg ggtgacgggg ctcggcagca ctctgctggt
      481 cgcgagcgtt gcgtacaccc tccttactgg catcggagtc tggggcaaca acatccccgt
      541 gggatgggct ttcgcgatca cgaacttcgt ctggtggatc gggatcgggc acgcgggcac
      601 cttcatttcg gcattcgtgc tcttgctgga gcagaagtgg cggacctcga tcaaccgctt
      661 tgccgaggcg atgacgctct tcgcgttggt gcaagccggt ctcttcccgc tgctccacct
      721 cgggcggagc tggttcttct attggctgat cccctacccg agcacgatgg gcgtctggcc
      781 acagttcagg agctcgctca cctgggacgt cgttgcgatt acgacttacc tgaccgtatc
      841 tctcttgttt tggtacctgg ggctcgtgcc ggatttggcg gcgctccgcg accacgtgcg
      901 cgaccgttgg ctcaaacgcg tgtacgccgt gttcgcgctc ggcttccgcg gctctgccga
      961 gaactggcgg cgtcaccggg tagcctacgg gctcatcggc gggctcgcgg cgccgctcgt
     1021 cgtctcggtg cacagcatcg tcagcatgga ctttgccatc ggcgtgctcc ccggctggca
     1081 ctcgaccatc ttcccaccgt acttcgtagc cggcgcgatc ttctcgggct tcgcgatggt
     1141 gctcacgctg ctgataccag cgcggcgctt gtatcggctc gagcaggtgg tcacccgtga
     1201 gcatctcgac aagatggcga agctcgtact cgtgacgagc atgatcgtga cctacgccta
     1261 cgcggtcgag acctattgcg cttggttggg tggagatacg ttcgacgcgc acatgcatct
     1321 cgtcgttcgg ccgaaaggga cgtacgctcc actcttttgg ctgcagatct gctgcaactg
     1381 tttggtgccg caactgctct ggtctcggcg cgcgcgccgc tccccggtcg ttctcttcgc
     1441 gctcagcatc ttcatccaga tcggaatgtg gacagagcgt ttcgtactca tcgtcacgtc
     1501 acagagcgaa gatttcctgc cttcgagcta tcgctcgtac gcgccgtccc tggtggatgc
     1561 ggcggtactc ggtggcacgc tcgtcttctt cgcgttcctg ttcttgctgt tcctgcgctt
     1621 cgtgccgttc atccccgtga gcgagctcaa ggccttgaag cacgagctcg gagactcatg
     1681 atgcgcaaga agactccgca gctcttcgcc gagttcgcta acgccgacca cgcgctccgc
     1741 gccgccgaag aattgcgccg ccggcacctg cgactcacga gcgcctattc cccattcgaa
     1801 atacccggcc tcgagcgata cgtacacacg tcgcggccct tcatgattcc ccttctgtcg
     1861 ctgctcgcgg cgctcggcgg gatggctctg gcttactcgc tcatttggtg gacggctgcg
     1921 ttcgactacc cactgaacgt cgggggacgt cccctgaatt cgtttcccgc cgacattccc
     1981 atattgttcg aaacgggcat tctgtgcgcg gcggtgtgcg ccttcatttg cgtgctcttc
     2041 ttctccgggc taccgcggct ggagcacgaa ctcgaggaga tcccgggctt cgagcgcacc
     2101 tccatcgacc gctattggct tggcatcgcg gcgacgggtt cactcccaga cgccgagctc
     2161 gaagcgctcc tgacgcgcgt cggcgcgctg cacgtgcatc ggctcgatgg tggtcggaca
     2221 tgatctcgct cgcgaacgaa cacggtttgg tagcagcgct gttcgcgacg ttgctcttcg
     2281 gatgtgacaa tcgccaggcg tttcggcgtc cagagccggg actcgaacgg atgctcaccc
     2341 agccgcgcag caagccgtac cgtgcgagca cgctctttgg cgacgggcgc gtcatgcgaa
     2401 ctcctcctcg agggagcgtt tcgcgcgaag cggtccacag cggcgacctg ctcgtgaccg
     2461 aaggtcgaac ggcgcaggga tacgcgactc gtgttccact gcgcctcacg acagaagtgc
     2521 tgcagcgcgg gcgcactgct ttcgagaacg tgtgcgcgac atgccacggc gtgctcggca
     2581 cgggggttag cttcgtcgcg gagaagatgg agctcagaaa gcccccttca ttgttcgagc
     2641 cgcgcatcgt cgcgttgccc ccggggcgcg tcttcgaaat cgtgagccgc ggttacggtc
     2701 tgatgccgag cttcgacgcc ctactcggca tcgacgctcg ctgggccgtg gttggctacc
     2761 tcgacgtgct tcgcgcgagc cgcgcgatca agctcgcgga gcttccgccc gacctgcagc
     2821 gccgcttcgc ggaggtcacg ccgtgaggcg cggcgtcatc gcgttgggcg ctctgggcgc
     2881 gctcggcgtc atcggctgcg gggcacaggc ttcgctccac gtcggcgtgg gtgcgccggg
     2941 ctacctcgcc gcgtttgcct tcctcacgag cacggtgctc ggcggcagct tgttgctgat
     3001 ggtcgccgag ctcagttccg cgtcttggtt cgtcgtcctg aggcgactcg ctgaagcgct
     3061 cgcgggcgtc actccgctcg tcgcggtcgc gttcgtgcca ttggctctcg ccttaccggc
     3121 gctctacccc tgggctcgtt cgttcgacgg gttggacgaa accctccgac gctcgctcga
     3181 acatgcgcgg ggttggatga atccccctct gttcctaggg cgcagcgcgc tcatcatcgt
     3241 gctctggatc gcgctcgcgg aaggcttacg ccgcgcctcg ctcggcgagg acgccggaca
     3301 gggcgaaggc tcgaagcgac gactcacgcg gctcagcgcg ctcggtgtgc cgctcgttgc
     3361 tttcaccggc tcgctcgcga tcttcgattg gttcatgtcc gcgcttccgg ggttcacgtc
     3421 gaatatcttg ggactgtatg tgctgtgcgg gggctttgca tcggcgatgg gggtcgtgtg
     3481 cgtggccttt cacgtggccc gcaaacgcgc tctctttccg agccgcgtcg cgcattatca
     3541 cacgcatgcg ctcgggcgaa tgttgctgat gtcagtgtgc ctgtgggcgt acatcgcggt
     3601 ttctcagctc gtcatcgtgt ggagcgccaa cttgccgaag gaggcgggct tctacctggc
     3661 gcgctaccgg gggccgttcc gcgctctcgc gatcgggctc gtattcgcgc actttctctt
     3721 gccttttctc cttcttttga gccgatggct caagcagagg tcgctcgcct tggctctact
     3781 cggcggcctc atcgtgctga tgcacgcggt ggacatgtac tggctcttgg ctcccgtctc
     3841 gccgcgtgcg gcaagcttcg tcgattgcgc gccattcctg ctgctcggag ccgtcgccgc
     3901 gagctttggt ctctggcgat tcagtcgcgc cccccgagtt ccgacccaag ccgccgagct
     3961 cgaacgctcg ctcgcgtacg aatccccatg accagtcgct ttcgcacgat tcaagagaac
     4021 gacagcgtac cattgggtcg ctcgctcacc gtcgtgctct tctcggtgtt ggtcggcgga
     4081 gccgcggtcg gcgcgtccgt ccttctgagt ggggcgcgaa tcccgaagcc gtcgtttgct
     4141 cctccgtcta gtgcgccgac gtcgatgccg gagcagtcgt tgatcgagcg aaccgctcgc
     4201 ggagctcacc tgcactcagc gcagcgcgcc gccctcgagc gctacgcctg ggtagaccga
     4261 gacgcgggga tcgtcagcat tccaatcgat cgagccatcg agctcaaagc cgagaaggag
     4321 ccctgatgct cgagctcttc cgacgcctat tcgcgttgcc gcggggcgcc tcgagcgtcg
     4381 ccgcgggcgt cgactggctg cattttctgg tgatcggcac caccttgctc ggcgcgctcg
     4441 gcatcggcgt cctcgcgacg gcgctcatca ttcgcaatcg aaacgaagcg cgtgggggag
     4501 caacgccgcg catcgccgcg agtcgccgtg aagaggcgct cgtcatcgcc ggcatcctcg
     4561 ggctgttttg cgccttttgg ctgattggct ttcggcagta tctcgaaatg cagcgacccc
     4621 cgtcgggtgc tcgccgcgtg tacgtaaccg cgaagcagtg gatgtggaag ttcaccgaca
     4681 tcgaagggcg gagttcgaac gatgtcttga cggtggaggt cggtgcgaag gtggagctcc
     4741 tgatgacgtc gcgcgacgtg atccacagct tcttcgttcc cgcattccgt ctcaagcgag
     4801 atgtcgtccc cggacgttac accgcaatct ggtttcaacc cgtcgagacc ggcgaattcc
     4861 ccatctattg tgccgagtac tgcggcgtcg cgcactccaa catggccggc gtcgtgcgcg
     4921 tgctctcatc ggccgactac ggccgctggc tgcaaggcgc cgaaccttcc aagggccaaa
     4981 gcctcgcgga ggtcggccgt gaagcggcag ttcgccatgc ttgctttgcg tgccacaccg
     5041 tcgatgggca accgcacatc ggccctacct ggagcggcct ctatggctcg acggtcgagc
     5101 tcgccgacgg tcaccgcgtc atcgccgatg cagcttacct cacccgctcg atgatggagc
     5161 cgagcgcgga gctcgtggcg ggttacccgc cggtcatgcc gacgtatctc ggctccgtat
     5221 ctcagcccga aacggcggcc ctcatcgaat acattcggtc cctccaacat acgaacccgg
     5281 cgcccagcgt gacgctgcct cgcctcgttt ctgagaccgc gcgcgccgcg gacgcgccgt
     5341 ccaacccagc gcctcgaggc ggcccggagc gagcgccatg actctagtct ccgagatcgc
     5401 gaacgctccg cgcgagccga actacgtcgg cgccaagacc agcgtacgct cttggttgct
     5461 cagcaccgat cacaagcgca tcggcatcct ctacttcgtc gccacgtcgc tcgcgctctc
     5521 gctcggcggg gtcttcgcac tgctcttgcg catcgagcac ctcacacccg agcgcaccat
     5581 cgtcgacgcg atcacgtaca accgcctgtt cacgctgcac gggatcacga tggtgtggct
     5641 cttcatgatc ccgagtatcc cggccgcgtt cggcaacttc ctcctgcctc tccagctcgg
     5701 cgcgagcgag gtcgcgtttc cgagactgaa ccttctgagc ttttggattt acgtcgtggg
     5761 cgccgcgttc gtgctcgccg cgctttggct cgggggggtc gacaccggtt ggacctttta
     5821 tgcgccgtac agcagcaaag cgccgtcggc ggtagtgccg gccgtaatcg ggatcttcat
     5881 cgtgggatgg tcgaccattc tcaccggcgt aaacttcatc gttacggtcc acacgatgcg
     5941 ctgcgccgga atcgggtgga tgcgtatgcc gctcttcacc tgggccatgt acggaaccag
     6001 cataatccag gtactggcaa caccggttct cggtctctcg ctgctaatcg tactggtcga
     6061 ccacctgttt tcatggggcc tcttcgatcc agcgcgcggc ggcgacccgg tgctctatca
     6121 gcacctcttt tggttctatt cgcatccggc cgtctacatc atgattttgc cggcgatggg
     6181 ggtggtgagc gaagtcgtct ccaccttcgc gcacaagaac ccgcactcgt atcgcgccat
     6241 cgtctactcg accttgggga tcgcgttcgt cggcttcctc acgtgggggc accacatgtt
     6301 cgtcgccggc atgtccacct tcgacgcggg cgcgttcggc gtcctcagca tgctggtcgc
     6361 aatcttcagc gccatcaagg tgttcgcctg ggtctcgacg ctacggcagg gtgccatttg
     6421 gctgaggact ccgctgctct acgtcatcgg tttcctcttc ttgttcgtgt tcggcggtat
     6481 gtccggcgtc gccgtcgcga cgatgagcct cgacgtgcac tggcacgaca cctacttcgt
     6541 cgttgcgcac tttcacttcg tgatggtggg cgcgacgctc accgcctggc tcgcagcaat
     6601 ccactattgg ttccccaagg tcaccggcaa gacgtattcg gagacgtggg gaacggtcgg
     6661 agcgttgctc gtctgcgtgg gtttggtcgc cacgttcttc ccgcagtttt tgcttggcaa
     6721 cgcgggcatg ccgcgcaggt acttcaacta cgacccgcgc tttcaagttt tgaacgtcgc
     6781 gtccacgctc ggctcctggg tgctcgcgac gggcatgctg gtcgcgttgg cggtgatcgt
     6841 gcatgccttg tttcgcggta ggccagcggg agacaacccg tggctctcgc gatgcttcga
     6901 gtggcgcacg agttcgcctc cgccggagaa gaacttcgcg cgggagcccg agctcagcgt
     6961 cgacgcttac gactacacga ctccatggcc ggagcagccc tcatgaactc ggtacatgct
     7021 gaacacttcg agtccgctcc aaagcaggcc cacgccgcgc gtctcggaat gtggatcttc
     7081 ctcgcgagcg agctcttgct gtttgccggg ctcttcgccc tatacggcgg ttaccgtgct
     7141 cactggcccg cggcgttccg cgagggcatt cggcactcga ccaaagcgct cggctcgctc
     7201 aacaccggta ttctcctcgt cagcagcacg tttgctgcgc tttcggtgca cgctacgcgc
     7261 gccggtcgaa ggtcgagatc gctcctcttg ctcgcgggca ctttcctgct cggccttttg
     7321 tttctcggca tcaagctcta cgagtactcc ctgcactttc gcgaagggat ctacccaggg
     7381 ggcagcggcg ccttcttcag cgcgcacccc gatcctggtc tcgtcccgtt ttggacgctc
     7441 tatttcgcaa ccacggggct ccacgccatc cacgtcagcg ttggactgat cgttttggga
     7501 tgcacttgct tcgcgctctg gcgcggcacg gttggcgtgc accgcgctca cccactcgag
     7561 aatgcggcgc tgtactggca tctcatcgac accatctgga tcttcgtctg gcctctctac
     7621 tacttggcgt gacgcagtgc ccaccaagca tcactcggaa cgtcgctccg ccatcgcccc
     7681 gctcgcggcc ggtgcgttgc tcctcattct cgccgttgca agctacgggc tgtcgttcgt
     7741 ctcgctgggc ttctgggcgt taccgattgc gttgagcatc gcggctctca aggcgtgcat
     7801 cgtcgtctcg atattcatgg agcttcgacg cgagaccgcc tcgatccagt tgacggccct
     7861 catggcggcg ctgctgttac tgatcctggt cgcgctcacc gccgccgacg tcgcgacccg
     7921 cggaaggtag cggctcgaat cgcccacgtt gcgcgcccgg gcttgcactg gtcgagcgta
     7981 cggacacccg aaagcgtacg aagaagcgca cggagcgtct gcgttcggca acccagttta
     8041 cggtgttgcg tggcgaaatt gatcttggtg tccccccaac gagcaacgct tcgctcgcgc
     8101 taacggtggt cactttcagg atctgaaagg tcggctcaaa atgtttttag tctagttcgg
     8161 tgcgcaacac gcgacgaaca gatcgatgcg gcgctcgctg gacatccagt agcgagacct
     8221 cactgcgctg gtataacgac cgcgttcttt ggcgcttgcc gctcgaacca tgcacatcgt
     8281 cggcatccag aaaatcattc cccagggtga gctgactgac c
//
LOCUS       DASTKS010001051         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1283116, whole genome shotgun sequence.
ACCESSION   DASTKS010001051 DASTKS010000000
VERSION     DASTKS010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283116"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VFQ35_22200"
     CDS             <1..1452
                     /locus_tag="VFQ35_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933440.1"
                     /translation="DGLSQLDETLFLDSDLLDVGVTALEGEANYLRYQSPAARRLCGI
                     HALYGLTEPSLVSVPDAALRRWHKDSDAVASIPPPDAQSPAAPRPMRACDDGRDFLDC
                     DFAPPTRPLLNAPQNSDAHGDYTLSWAPGSQFILEEALRPDYADATTIYAGPRQSIEF
                     RGHTPGNFYYRVRSVRDGLSSAWSEGRLVHVQPPLLYRTEARTDYRPDILLAVQRALL
                     RLCAVRGDMMAVLCAPEGVTELDAIAHAGVLRSFATTTSTLVPPLGQGEASALSFGAL
                     YHPWLIGRDTDRSLELRTSPPDGAATGVIARRTIARGAWLAPANELLEGVVALARPAA
                     SERMQSLYDAQINVFSKKPRGFLAEGADTLSLDEDLVPIGVRRLMSLLRRAALEAGQV
                     HVFEPNDPAFRRLIERNFEALLTNLYTRGAFAGKTTASAFQVVADATINSAASVDQGR
                     FFVELRVAPSLPLTFLTVRLLQDGAQSRVTEGA"
     gene            1453..1932
                     /locus_tag="VFQ35_22205"
     CDS             1453..1932
                     /locus_tag="VFQ35_22205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET9933441.1"
                     /translation="MANPANDTTVYPFTSFNFSIEINVPGISSRACNAAFAECDGLEI
                     TMDVKTIREGGNNGRQVRLSGPQNFGQLTLKRGMTASFELWDWVAGVLENPGLRADAE
                     VVLLAPDGQKERARFLLSRCLPIKLKAPPLNSKDGMVAVEELQLAYESLRLKKPGAQ"
     gene            1932..>2234
                     /locus_tag="VFQ35_22210"
     CDS             1932..>2234
                     /locus_tag="VFQ35_22210"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933442.1"
                     /translation="MAKLAKAELRKLDANFENEIEPENWAVVQFNPETLKVSFANQVA
                     TPSGSGDQRGTPARQFVGAGTTKLAVQLWFDVGAQQGETDAVDDVRKLTKKVAHYIT"
BASE COUNT          410 a          750 c          685 g          389 t
ORIGIN      
        1 gacggcctgt cacagttgga tgaaacgctc ttcctcgact ccgacttgct ggacgtcgga
       61 gtgaccgccc tcgaaggcga ggccaactac ctgcgctacc aaagcccggc cgcccgtcgt
      121 ctgtgcggca ttcacgcgct ctatgggctc accgaaccga gcttggtttc ggttcccgac
      181 gccgcgctcc gacgctggca caaggactcc gacgcggtcg cgtcgattcc gccgcccgat
      241 gcgcagagcc cggccgcacc acgtccgatg cgcgcttgcg acgatgggcg cgacttcttg
      301 gactgcgact tcgccccccc tacccggcca ctgctcaacg cgccccagaa ctcggacgcc
      361 cacggcgact acacgctgag ctgggcgccg ggcagccaat tcatcctcga ggaagcgctg
      421 aggccggact acgcggacgc gaccacgatc tacgcggggc cgcgccaaag catcgagttt
      481 cgcggccaca ctcccggcaa cttctactat cgagttcgga gcgttcgaga cggactatcg
      541 agcgcgtggt cggaaggacg gctcgtccac gttcagccac cactgcttta ccgcaccgaa
      601 gcgcgtaccg actaccgacc cgacattttg ctcgccgtgc aacgcgcgct cttgcgcctt
      661 tgcgccgtgc gcggcgacat gatggccgtc ctgtgcgcac cggagggtgt caccgagctc
      721 gatgcgatcg ctcacgcggg agttctgcgc tctttcgcga cgacgacttc gacgctggtg
      781 cctcccctcg ggcaaggcga ggcgagcgct ctttccttcg gcgccctcta tcatccctgg
      841 ctcatcggaa gagacaccga tcgctctctc gagctccgca ccagcccccc cgacggcgcc
      901 gcgaccggcg tcatcgcgcg acgcacgatc gcgcgcggcg cttggctcgc gcccgccaac
      961 gagttgctcg aaggcgtcgt ggccctggcg cgccccgcag cgagcgaacg catgcaatcg
     1021 ctctacgatg cgcagatcaa cgtcttctcg aagaagccgc gcggcttcct ggcagagggc
     1081 gccgacacgc tgagtctgga cgaagacctc gttcccatcg gtgtccgacg attgatgagc
     1141 ttgttgcgcc gcgccgcgct cgaggctgga caggtccacg tgttcgagcc gaacgatccc
     1201 gcgtttcgca ggctcatcga gcgaaacttc gaggccttgc tcacgaacct ttacacgcgc
     1261 ggcgcgttcg caggcaaaac gacggcgagc gcgttccagg tcgtcgcgga cgcgaccatc
     1321 aactccgctg cgtcggtcga ccaaggacgc ttcttcgtcg agctgcgggt tgcgccttct
     1381 cttccgctca ccttcctcac cgtgcgcttg ctgcaagacg gtgcgcaaag ccgcgtaacg
     1441 gagggcgcgt aaatggcaaa ccccgccaac gacacgaccg tttatccgtt cacgtccttc
     1501 aacttctcca tcgagatcaa cgtgccgggg atcagctctc gagcgtgcaa cgccgccttc
     1561 gccgagtgcg acggcctgga aatcacgatg gatgtgaaga ccatccgcga gggtgggaac
     1621 aacgggcgcc aggttcgtct gtctgggccg caaaacttcg ggcaattgac gctcaagcga
     1681 ggaatgacgg cgagcttcga gctgtgggat tgggtagcgg gagtgctcga aaatccgggt
     1741 cttcgcgccg atgcggaggt cgttttgctg gcccccgacg ggcaaaaaga gcgagcgcgc
     1801 ttcctcctat ctcgctgttt gccgatcaag ttgaaggctc ctcccttgaa ctcgaaggac
     1861 gggatggtgg cagtagaaga gcttcaactc gcgtacgaat cgctgcgtct caaaaagcca
     1921 ggagctcagt gatggccaag ctcgcaaaag ccgaactccg aaagctcgac gcgaatttcg
     1981 aaaacgagat cgagcccgag aactgggccg tcgttcagtt caacccggag acgctcaagg
     2041 tgagtttcgc caatcaggtg gcgacgccgt cggggtcagg tgaccaacgc ggcaccccag
     2101 cgcgtcagtt cgtgggcgcg ggcacgacca agctcgcggt tcagctgtgg ttcgatgttg
     2161 gcgcgcagca aggcgaaacc gacgccgtcg acgacgtgcg taagctgacg aagaaggtcg
     2221 ctcactacat cacg
//
LOCUS       DASTKS010001052         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1059523, whole genome shotgun sequence.
ACCESSION   DASTKS010001052 DASTKS010000000
VERSION     DASTKS010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059523"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /gene="uvrC"
                     /locus_tag="VFQ35_22215"
     CDS             complement(<1..1465)
                     /gene="uvrC"
                     /locus_tag="VFQ35_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9933443.1"
                     /translation="MDPIDPTDPLEDKLGLLPAAPGCYVFRDSKGEVLYVGKAKSLRS
                     RVRSYFQDSNSDTRAFLPFLRRQVSDFETIVTGSEKEAAILENHLIKERKPRYNVKLR
                     DDKEYLNLRLSSEHEWPRLELVRRPKPDGARYFGPYHSATAARRTLSVVEKHFKLRTC
                     TDRELASRKRPCLEYQIKRCPGPCVLEVDKEAYAAQVHAVALFLAGRHDELTRELKSR
                     MSEASERLEFELAATYRDQISAVESVRQAQRVAQVSDQDQDVLGIYREGDLVELSLMI
                     VRGGRVIDVGSFSNRRVEVPNDELVAAFLREHYGEGGGGEALIPDEILLPVLPEGVEG
                     VMDWLSEQRAARAAAEGVRAPRRVEILSPVRGPRRGLLDLAAQNAEHSFKEKRRSHDD
                     IDERLLRVQARLRLPTLPRRIECVDISHLGGTDTVGAVVALENGALDKKRYRTYHVKG
                     VPEGDDYGAIYHVLSRRFRRGQRALLEAESRAQPSAAV"
     gene            1591..3216
                     /locus_tag="VFQ35_22220"
     CDS             1591..3216
                     /locus_tag="VFQ35_22220"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9933444.1"
                     /translation="MALLPRALTSRTGAAIGVAVAAGLGIGLLLVPRGPGPGDDKEPL
                     PETLLLGKALPADEQAHKVALSRARAFLGGRFQLTLPDGTEREVYLGQLGAELDKVYL
                     SNLVRDAHDRTSSLLRVFRASESKGPLVLPLPITLDPTLAVPTLLALKDQVDRVPVDA
                     RLDLEKRELVKETYGRLLDVDASLRNIQTALRAGKTKVELAFQEQRPRRVASELGNVE
                     FGQVLGFFETRYDRSQRQEARTYNLRLAASRLDGYVLLPGEVFDFNAVVGPRDEANGY
                     KVAPVIAEGELVDGIGGGTCQISGTLHGAAFFAGLSIVERYPHTRPSAYIKMGLDATV
                     VYPTINFRLKNPFPFPIVFHETVKNGVVRAEVLGPKRALTVTFIRRVLDAIPYEEVER
                     PDKDLPRGMRVLGQRGVPGFKLRRYRIVRDGDHAVRDRWDDLYPPTTQIVRVGTGDSG
                     DTPLKATDDQHPEYLADELLIMTQGVDADEEGSSNLVENREPGRFGENGWTEAAGMPY
                     FRAQHGDRAAERPERDSKSKRKSRKTGRSDKQT"
     gene            3484..4128
                     /locus_tag="VFQ35_22225"
     CDS             3484..4128
                     /locus_tag="VFQ35_22225"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9933445.1"
                     /translation="MMNTETVATARKPTEENGSARSADWDNPRVASILSAAAKCFARK
                     GFTATTLAEIGKELGLRKSIVHYYFASKAALIHEVQSFTYHRYLDRLKEAIRNGDGSS
                     QQATDALRALWDAIQNNKTGTGLNIEVWSAARRDAELKRRAAGLQRDARQVIRDAIAP
                     VTQKAGPARAEALATLALAVLNGLSVTEYLEGEEAKVQEAYSTFLELLRSGFDS"
BASE COUNT          826 a         1513 c         1402 g          777 t
ORIGIN      
        1 cgaccgctgc ggacggttgc gcgcgactct cggcctcgag cagcgcgcgt tggccgcgtc
       61 ggaagcgccg cgaaagcacg tgataaatgg ctccgtagtc gtccccttcc ggcacgccct
      121 tcacgtggta ggtacggtac cgcttcttgt cgagcgcgcc gttctcgagc gcgacgactg
      181 cgcccacggt gtcggtgccg ccgaggtgag agatgtcgac gcactcgatg cggcggggca
      241 gcgtcggcag gcgcaatcga gcctggacgc gcagcagtcg ttcgtcgatg tcgtcgtgtg
      301 agcgccgctt ctccttgaac gagtgctcgg cattttgcgc ggccagatcc agcaagccgc
      361 gccgcggacc gcgcactgga gagaggatct cgacgcgccg tggcgcgcgc actccctcgg
      421 ccgccgcgcg cgcggcgcgc tgctcactga gccaatccat cacgccttcg acgccttctg
      481 gcagtaccgg caacaagatc tcgtcgggaa tcagcgcctc ccctcccccg ccctcgccgt
      541 agtgttcgcg cagaaacgcc gcgacgagct cgtcgttcgg cacttcgacg cggcggttcg
      601 agaagctccc gacgtcgatg acccggccac ctcgcacgat catgagcgac agttcgacca
      661 aatcaccttc tcggtagatg ccgagcacgt cttgatcttg atctgaaacc tgcgccaccc
      721 gctgcgcttg ccgcacgctt tcgaccgccg agatttggtc tcgatacgtc gccgcgagct
      781 cgaactcgag ccgttcgctc gcttcgctca tgcgtgactt gagctcacgc gtgagctcgt
      841 cgtggcgacc cgcgagaaat agcgccactg cgtgcacctg cgcggcatac gcctccttgt
      901 cgacctcgag aacgcacggc cccgggcagc gcttgatttg gtactcgagg cacgggcgct
      961 ttcggctcgc gagctcgcga tccgtgcagg tgcgcagctt gaaatgcttc tcgacgacgc
     1021 tgagcgtgcg tcgcgcggcc gtcgccgagt gatacggacc gaaatagcgc gcgccgtcgg
     1081 gctttggtcg ccgcacgagc tcgagccgag gccattcatg ctccgacgac agccgcaggt
     1141 tcaagtactc tttgtcgtcg cggagcttga cgttgtagcg aggcttacgc tccttgatca
     1201 ggtggttttc gaggatcgcc gcttctttct cgctgccggt gacgatcgtc tcgaaatcgc
     1261 tcacctgccg tcgcaaaaat gggaggaaag cgcgagtgtc gctgttcgag tcctggaagt
     1321 agctgcgcac acgcgagcgc aagctcttgg ccttgccgac gtagagcacc tcgcctttcg
     1381 agtcgcgaaa gacgtagcaa cccggagcgg ccggcaataa gccgagcttg tcctccaggg
     1441 ggtcggtggg gtcgatcgga tccatcgcgc gttcactgtc acctacctcg caccgcgcgc
     1501 tctgcccagc ccgagcaggt tcgcccttcg aacaatccgc tcgggtcctg ccgacttgac
     1561 gcagaccgga tctcgaaaaa tagaagaccc atggcgttgc tcccccgtgc cctcacgagt
     1621 cgcaccggcg ccgccatcgg cgtggcggtt gcagccgggc tcggcatcgg gctcttgctg
     1681 gttccgcgcg ggcccggtcc gggcgacgac aaagagcccc ttcccgagac tttgttgctc
     1741 ggaaaagcgt tgcccgcgga cgagcaggca cacaaggtcg cgctgagtcg cgcccgagcg
     1801 ttcctcggcg ggcgctttca gctgacgcta cctgacggca ccgaacggga agtctacttg
     1861 ggccagctcg gcgccgagct cgataaagtc tacctctcga acttggttcg ggacgctcac
     1921 gaccgcacga gctccctgct tcgagtcttc cgtgcctccg agagcaaggg gccgctcgtg
     1981 cttccgcttc cgatcacgct cgacccgacg ctcgcggtcc ccacgctgct cgcgctcaag
     2041 gaccaggtcg accgagtgcc ggtcgacgcg cgcctggatc tcgagaagcg tgagctcgtc
     2101 aaagagacct acggtcgcct gctcgacgtc gacgcgagcc tccgcaacat tcaaacggcg
     2161 ctgcgcgcgg gcaagaccaa ggtggagctc gccttccagg aacagcggcc ccgacgcgtc
     2221 gcgagtgaac tcggcaacgt ggagttcggg caagtgctcg gtttcttcga gacgcgctac
     2281 gatcgctcgc agcgacaaga ggcgcgcacg tacaatctcc gtctcgcggc gtcgcgcctc
     2341 gacggctacg tgcttcttcc aggagaagtc ttcgacttca acgccgtcgt cggtccgcgc
     2401 gacgaagcca acggctacaa agtcgcccct gtcatcgccg agggtgagct cgtcgacgga
     2461 atcgggggag gcacttgcca gatctccgga acactgcacg gcgccgcgtt cttcgctgga
     2521 ctctcgatcg tggagcgtta cccgcacacc cggccgagcg cgtatatcaa gatggggttg
     2581 gacgcgacgg tcgtctatcc gaccatcaac ttccgtctga agaatccgtt cccctttccc
     2641 atcgtgttcc acgagacggt gaagaacggc gtcgttcgag cagaagtact cgggcccaag
     2701 cgcgcgctca cggtcacctt cattcgtcgc gtgctcgacg cgatccccta cgaggaagta
     2761 gagcgtcccg acaaagatct acctcgaggt atgcgtgtgc tcggacagcg cggcgttccg
     2821 ggtttcaagc tgcgtcgcta ccgaatcgtg cgcgacggcg atcacgcagt gcgcgatcgc
     2881 tgggacgacc tgtacccacc cacgacgcag atcgtccgcg tggggaccgg cgactcgggt
     2941 gatacgcccc tgaaggcgac ggacgatcag catcccgaat acctcgccga cgaactgctg
     3001 atcatgaccc agggcgtcga cgcggacgaa gaggggtcct caaatctcgt ggaaaatcga
     3061 gaacctggac gatttgggga aaacgggtgg accgaggcgg ccggtatgcc ctactttcgg
     3121 gcccaacatg gagatcgggc ggccgagcgg cctgagcgag actcaaaaag caagcggaaa
     3181 tcccggaaaa ccgggcgttc cgacaagcag acttgactcg tgggtcgacc tgcgcgaaga
     3241 acgccgcccc gggagagggc ggttgcccga cccggaaggt ctttccgaac cgcatcggtc
     3301 ggccagtccg gaacagcccg gcggccgcta cttgacagtc ggccagtccg ggaacaatcc
     3361 ggcggccgca cacgaagagt cggccagtct ggaaccaagc cagcagccgc cacttgacag
     3421 tcggccggcc ctggcaaaat caggcggccg cgtataaacc agtcggccgg acatcaataa
     3481 gccatgatga acacagaaac tgtcgctacc gctcgtaaac ccactgaaga aaacggctcg
     3541 gcgcgcagcg ctgactggga caacccgcga gtcgcttcaa tcttgtcggc cgcagccaag
     3601 tgcttcgcgc gcaaaggctt caccgcgacc acgctcgccg agatcggcaa ggagctcggt
     3661 cttcgcaaga gcatcgttca ctactacttc gcgagtaagg ccgccctcat tcacgaagtt
     3721 cagagcttca cctaccaccg ctacctcgac cgcttgaagg aagcgatccg caacggtgac
     3781 ggctcgtcgc agcaagcgac tgacgcgctc cgtgcattgt gggacgcgat tcagaacaac
     3841 aagaccggca ccggcctcaa catcgaggtg tggagcgctg cgcgtcgtga cgcagagctc
     3901 aagcgccgcg ccgcgggcct gcagcgggat gctcgccagg tcatccgcga cgccatcgcg
     3961 ccggtcacgc aaaaggctgg ccccgctcgc gctgaagcgc tcgcgaccct cgcgctcgcc
     4021 gtattgaacg gcctgagcgt caccgaatac ctcgagggcg aggaagccaa ggttcaggaa
     4081 gcgtacagca ccttcctcga gctcttgcgc tccggcttcg acagctgaag ccgcggggac
     4141 gcgttaaacc cccagaacct atcccgagaa ggcgcagcgc ctcgggccgc gccgccaagg
     4201 tcggcccgct gggataggtt cttcgaggcg gtgagcgctc cgccaacgaa cgcccaagcg
     4261 tcgaggcatc gcagaatcgc gagccgtttc ggctttcgcc ttgacgtagg aagaccccgc
     4321 acccaggact tttccgagca aacttgtttc gaaaacttct ggcactgatg acggctgccg
     4381 cgctctggct ctcggcggcc gaagcctccg ctgcgttgcg gcctcacaaa gcgcgtctcg
     4441 gtttcatggg aatgctcccc gtgtccgaga cgagttggct ggttggtggg ctcgaacgcg
     4501 gccaaacctt cgagcttt
//
LOCUS       DASTKS010001053         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_166763, whole genome shotgun sequence.
ACCESSION   DASTKS010001053 DASTKS010000000
VERSION     DASTKS010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166763"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1236)
                     /locus_tag="VFQ35_22230"
     CDS             complement(361..1236)
                     /locus_tag="VFQ35_22230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9933446.1"
                     /translation="MTDPNKKSTRTFQCREALWQKFEQMAKELECSVDYLINDAMKQY
                     ARQRGYATATSLGAMPSMPNAAAAPGSSPVAAPPPAHVAPPPPPPQVHAPPPPLPPPH
                     AAPHAPPPPPPPPPTGHSVVAPPPPPVGRPTPPPKRGPTPPPPPPSMGAPPPPVGGPP
                     PPPPPLGRPGAPPPRGMPSRSLPPLPGGAPPPMARPAAPGSLAIHYQGEVFPVNKDRF
                     IIGRGKQTSDLTIKDPNVSRQHAMIEFSNGQYYIVDMGSTNGVEFAGQRVQRRAIGDG
                     DIFKVCDHELRFSYQ"
     gene            complement(1356..>2448)
                     /locus_tag="VFQ35_22235"
     CDS             complement(1356..>2448)
                     /locus_tag="VFQ35_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933447.1"
                     /translation="DALASWSATNFAERLDDSSQLYGIEQMMRELRADGVRSLPNLIA
                     PGAPKIDQLAGLIADFGDPQTKLAASQKLVAVAKEVNSENWIKQKAPSVEAANRASKL
                     NPTPEQFRKQLVQYQEEELLRVFASMKKVGGKAIVDYLLAFTQDKNESEKRRATAMAA
                     LQGNLDPKNPAHAEVPLQLAASNDTPDQLRDVAFARVGEFPRQMILGKLYSFFKTDNW
                     KVRWVAAELILKTSDTSELPEFFSKLGQAEGFSITEPLRYGALIAAMKGSPPPAEVVD
                     KYATPSHPVPVRMSALGYYFEKGTKADLGKVEQYAADKAKAPTCKESAKECEWKCEVT
                     AGDKHETKEITTLGEFVTYCIKPAMEARK"
BASE COUNT          398 a          779 c          828 g          443 t
ORIGIN      
        1 gtcgccgcac gcgctctcac cacgacgccc tcacgtcacc acgaacgcgc gcagcgcgga
       61 gcgccacaac gccctcagct cgccggccct cacgtcacca cgaacgcgcg cagcgcggag
      121 cgccacaacg ctcctctcac cacgacgccc tcacgtcacc acgaacgcgc gtagcgcgga
      181 gcgcccccgt cctcaaaaca agccaccacg catcaacaag cccgcccgcg aagttttgcg
      241 ttttggcaag gctttcgcga gcatcggagc ggaacgtacg accgtacgtg agcaccgaag
      301 cgcagcgaaa acgccgccaa aaccaaaaat ccgcgggcgt cccctcaggt cgccacgacc
      361 tcactgataa gagaagcgga gctcgtggtc gcagaccttg aagatgtccc catccccaat
      421 ggcgcgacgc tggacgcgct gcccggcgaa ctcgacgccg ttcgtcgagc ccatgtccac
      481 gatgtagtac tggccgttcg agaactcgat catcgcgtgc tgacgcgaga cgttcgggtc
      541 cttgatcgta agatcgctcg tttgcttgcc gcggccgatg atgaagcggt ccttgttcac
      601 ggggaacact tcaccctggt agtgaatggc gagcgagcct ggagccgcgg ggcgagccat
      661 gggcggaggc gcgccaccgg gtaacggagg caagctgcgt gacggcatgc cgcgcggggg
      721 cggggcaccg ggacgaccga gtggcggcgg cggaggcgga ggtcccccaa ctggaggcgg
      781 cggcgcgccc atggagggag gtggcggggg aggcgtcggg ccgcgcttgg gcggaggcgt
      841 cgggcgaccg actggcggcg gagggggcgc gaccaccgaa tgccccgttg gcggaggcgg
      901 cggtggtgga ggcggcgcgt gcggagccgc gtgagggggc ggcaacggtg gaggcggcgc
      961 gtgcacctgc ggtggcggcg gtggcggcgc gacgtgagcg ggcggcgggg ccgcaaccgg
     1021 cgacgaaccg ggtgctgcgg ccgcgttcgg catcgacggc atcgccccca ggctggtcgc
     1081 ggtcgcgtac ccgcgctgcc gcgcgtactg cttcatcgca tcgttgatga ggtagtcgac
     1141 ggagcactcg agctccttcg ccatctgctc gaatttttgc cagagcgcct cgcggcactg
     1201 gaaagtccga gtcgatttct tgttcggatc ggtcatcaca ggtctcgttc tgcggcgcgc
     1261 cagagctggg cggataaccc cgaacgcata tagcagctaa cccgcctgct cagcgactag
     1321 ctgtacgcgt ttcgacgagt tcggggtgca tccgctcact tcctcgcttc catagcgggc
     1381 ttgatgcagt aggtcacgaa ttcgccaagg gtggtgattt ccttggtctc gtgtttgtcg
     1441 cccgcggtca cttcgcactt ccactcgcac tctttggcgc tctctttgca cgtcggcgcc
     1501 ttcgctttgt ccgcagcata ttgctcgacc ttgccgagat cggccttggt gcccttctcg
     1561 aagtagtagc cgagcgcgga catgcgaact ggcactgggt gcgacggggt ggcgtatttg
     1621 tcgacgactt cggccggcgg cggcgagcct ttcatcgcgg cgatgagcgc cccgtagcgg
     1681 agcggctcgg tgatggagaa gccttcagcc tgtccaagct tgctgaagaa ttcgggcaac
     1741 tcgctcgtgt cgctcgtctt gaggatgagc tcggcagcca cccagcgcac cttccagttg
     1801 tcggtcttga agaaggagta gagcttcccc aagatcatct ggcgggggaa ttctccgacg
     1861 cgagcgaacg cgacgtcgcg tagctgatcc ggcgtgtcgt tcgatgccgc aagctgcagc
     1921 gggacctcgg cgtgggcagg gtttttggga tcgaggttgc cctgcagcgc ggccatcgcg
     1981 gtcgcgcgcc gcttttcgct ctcgttcttg tcctgggtga acgccaagag ataatcgacg
     2041 atcgcttttc cgccgacctt cttcatcgat gcgaacacgc ggagcagctc ttcctcttgg
     2101 tactgaacga gctgcttacg gaactgctcc ggagtcgggt tcagcttcga agcgcggttc
     2161 gcggcttcga cgctgggcgc cttttgcttg atccaattct cggagttgac ctctttggca
     2221 acggcgacga gcttctggct cgccgcgagc ttggtctgcg gatctccgaa atctgcgatg
     2281 agcccggcga gctggtcgat cttcggggcg cccggcgcga tcaggttcgg gagcgagcga
     2341 acaccgtcgg cccggagctc gcgcatcatc tgctcgatgc cgtagagctg cgaagagtcg
     2401 tccaagcgct cggcgaagtt ggtcgccgac cagctcgcca acgcgtct
//
LOCUS       DASTKS010001054         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_388888, whole genome shotgun sequence.
ACCESSION   DASTKS010001054 DASTKS010000000
VERSION     DASTKS010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388888"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFQ35_22240"
     CDS             <1..373
                     /locus_tag="VFQ35_22240"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HET9933448.1"
                     /translation="LRTGYAGGFLHPTTGYSFPVAARLASFIARRPPDALFGADFDKF
                     LREHARQLRFGELLNRLLFQAFEPERRRAVFERFYRLPQETIARFYALETTSLDRTKI
                     LCGRPPRGLSLKRALLGGLHP"
     gene            370..1395
                     /locus_tag="VFQ35_22245"
     CDS             370..1395
                     /locus_tag="VFQ35_22245"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9933449.1"
                     /translation="MSQLARSLEPENLALRLLAQRFRERGATPGGDGVAYSLWEKALL
                     EPLCDFLARPGKEFRARMVALAWELSGRHGGPPPELSLLVEAIHAGSLIVDDIEDDST
                     YRRGGRALHLDYGLPRALNAGNWLYFWPADLIAELGLPPASELALHRVIGRTMLACHE
                     GQALDLSTRVTELEQAEVPIVVLATTRLKTGKLFELAAAVGAIAAGAPSSTVRAVSEF
                     GMALGVGLQMLDDLGGIVSEHRCHKGHEDLLAARPAWPWAWVAETTEPHVYAHLRVLG
                     RQVNARERHPELLATELRGLIEADGRKRVDEHLRQAFGDLSHKVGRSRALDEALNEIQ
                     TLEKSYG"
     gene            1388..2935
                     /locus_tag="VFQ35_22250"
     CDS             1388..2935
                     /locus_tag="VFQ35_22250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HET9933450.1"
                     /translation="MVDAAPASGKTRKTRVAVVGSGFGGLAAAIRLAVNGYAVTLFEA
                     RDKPGGRAYVYEQDGYVFDAGPTVITAPDCIDELFRIAGRRREDYVEFLPVTPFYRLL
                     WEDGTRFDYDGSSESMARQIERLDPEDCAGYERFVEYSKRVFEKGYEELAASPFLSFF
                     DMVRVAPHLARLRADRSVYGTVSRFVRSPYLRQALSFHSLLVGGNPLDTSSIYTLIHY
                     LERKWGVWFPRGGTGALVRALVRLFEELGGELRLKTPVRGIRLVEREGTTLHSISSGE
                     QTEELFDLVVSNADVHHTYARLYADVPAAQRTQRKLERSEWSMSLFVLYFGTDRPYDL
                     AHHTVLFGPRYEELLKEIFEGPELPPDFSLYLHAPSVTDASLAPPGGATFYVLSPVPH
                     LGNAPIDWEKQAPIYKDRVYQALERHMPELRKHVVTERWFSPQDFVTELNAFQGSAFS
                     VAPKLTQSAYFRPHNRDPNIAGLYLVGAGTHPGAGLPGVINSAKATVSVILRDFSAAS
                     DLERRTA"
     gene            2932..3894
                     /locus_tag="VFQ35_22255"
     CDS             2932..3894
                     /locus_tag="VFQ35_22255"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET9933451.1"
                     /translation="MNSATLALPHDTSADCAEALRQGSKSFALAGRLLPPQIARDAAT
                     LYTFCRRVDDTVDLAAPGSEQRALDAVWLRVRSVFRGESQSDPFWASFAELVRRVNLP
                     ERYVIELLLGMQMDVDGTRYETVDQLLLYCHRVAGVVGLMMCHVLGVRTDAALRNAAH
                     LGIALQLTNISRDVQEDWERGRMYVPAELLRKAGLPQLEPEPRALTRTESNALSLALA
                     ELAKLAERFYRSGDDGLPALSARARFAVRTARLVYSHIGVLLQRRDYDVLAGRVIVPA
                     YRKLVLLLRAALVALVEWLLPRRRAAVRLPERVLRFPEDVLPAP"
     gene            3905..4561
                     /locus_tag="VFQ35_22260"
     CDS             3905..4561
                     /locus_tag="VFQ35_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933452.1"
                     /translation="MPASSPIKLRVSNAPELARLREAEPPPHTSFLKEVAIYVGRARQ
                     FNRTDWLVYVSWVGLIFGLFGATLGFLGVGHAHGVSYPAEAWLLPIGALIFAISIAVD
                     TIGHRTIYKEEISRAEGLVHHITIFSGITSCVLLCLAYTYPRALWVPALVMTALSLLY
                     SLIDEAFHWRRYVQKFSDRVEMWSHVGILTGHTTLMIAWWWWFFDGYRGVAETLPFLL
                     "
BASE COUNT          757 a         1605 c         1519 g          893 t
ORIGIN      
        1 gttgcgcacg ggatacgcgg gagggttcct gcatccgact acgggctact cctttccggt
       61 ggcagcgcgc ctcgcgagct tcatcgcgcg gcgcccgccc gacgcgctat ttggcgccga
      121 tttcgacaaa tttctgcgcg agcacgcccg ccagctgcgg ttcggcgagc tcctgaatcg
      181 gctcctgttc caagccttcg aacccgagcg acgtcgcgcg gtgttcgagc gcttctaccg
      241 attgccccaa gaaaccatcg cgcgcttcta cgcgctcgaa acgacgtcgc tcgaccgaac
      301 caagatcttg tgtgggcgtc cgccgcgcgg actctctctc aaacgtgcac ttctcggagg
      361 actgcatcca tgagtcagct cgctcgttcc ctagaaccag agaacctcgc gcttcgcttg
      421 ctcgcgcagc gcttccgcga gcgcggcgcg acgccgggtg gagacggcgt tgcctactca
      481 ctttgggaga aggcgctgct cgagccgcta tgcgacttcc tcgctcggcc cggcaaagag
      541 ttccgtgcgc gaatggtcgc gctcgcttgg gagctctcgg gtcgtcacgg tggacctcca
      601 cccgagctct cgctcttggt ggaagcgatc cacgcgggct ctttgatcgt cgacgatatc
      661 gaagacgact cgacgtaccg tcgcggaggc cgcgcgctgc acctcgacta cggcttgccg
      721 cgcgcgctga atgcgggcaa ctggctctat ttctggccgg ctgatctgat cgccgagctc
      781 ggtctgccgc cggcgagcga gctcgcgctc caccgcgtga tcggccgcac catgctggcg
      841 tgtcacgagg gtcaggcgct cgatttgtcg actcgcgtga cggagctcga gcaggccgaa
      901 gtgccgatcg tcgtgctcgc gacgacacgc ttgaagacgg gcaaactgtt cgaactcgcc
      961 gccgccgtcg gggcgatcgc cgccggcgcg ccttcatcca cggtgcgtgc ggtctccgaa
     1021 ttcggcatgg cgctcggcgt cggtctgcag atgctcgacg atctcggagg gatcgtcagc
     1081 gagcaccgtt gccacaaggg tcatgaagat ctcctcgccg cgcgcccggc ctggccttgg
     1141 gcttgggtcg cggaaacgac cgagccgcac gtgtacgcgc acctgcgcgt gctcgggcga
     1201 caagtgaacg cacgcgagcg tcaccccgaa ctcttggcga ccgagctgag aggcctcatc
     1261 gaggccgacg gacgcaagcg cgtcgacgaa cacttgcgcc aagctttcgg cgatctctcc
     1321 cacaaggtcg gccgttcgcg cgcgctcgat gaggcgctaa acgagattca gacgttggag
     1381 aagagctatg gttgatgccg cccccgcttc cgggaaaact cgcaagactc gtgtcgccgt
     1441 cgtcggcagt ggtttcggcg gcctcgcggc tgcgatacgc ctcgccgtga acggttacgc
     1501 ggtgacgttg ttcgaagcgc gcgacaagcc gggtgggcgc gcttacgtct acgagcaaga
     1561 cgggtacgtc ttcgacgcgg ggcccaccgt gatcacggcg cccgactgca tcgacgagct
     1621 ttttcgcatc gcagggcgac gtcgagagga ctacgtcgag ttcctgccgg tcaccccgtt
     1681 ttaccgtctg ctctgggagg acggcactcg cttcgactac gacggcagct cggagtcgat
     1741 ggccaggcag atcgagcgcc tcgatccgga agattgcgcc gggtacgagc gattcgtgga
     1801 gtattcgaag cgcgtgttcg agaaagggta cgaggagctc gccgcgtcgc cattcttgag
     1861 cttcttcgac atggtgcgcg tggcccctca cctggcccgg ctccgcgccg atcgctccgt
     1921 gtacggcacg gtgtcgcgct tcgttcgcag cccctacctg cgccaggccc tgagctttca
     1981 ttcgctcttg gtaggcggca acccgttgga cacgagctcg atctacaccc tcatccacta
     2041 tctcgaacga aagtggggag tctggttccc gcgcggcgga acgggagctc tcgtgcgggc
     2101 gctggttcgg ctcttcgaag agctgggcgg tgagctcagg ctgaagaccc cggtgcgcgg
     2161 cattcgtctc gtcgagcgcg aaggcaccac cctgcactcg atctcgagcg gcgagcaaac
     2221 cgaagagctg ttcgatctcg tcgtatcgaa cgcggacgtc caccatacgt acgcgcggct
     2281 ctatgcggat gtgcccgccg cgcagcgcac gcagcgcaag ctcgagcgca gcgagtggtc
     2341 gatgtcgctc ttcgttctct acttcggcac tgatcgccct tacgatctcg cgcaccacac
     2401 ggtcctgttc ggcccgcgct acgaagagtt gctgaaggag atcttcgagg gccccgagct
     2461 cccgcccgac ttcagcttgt acctccacgc accgagcgta accgacgcct cgctcgcgcc
     2521 ccctggcggc gccaccttct acgtcctcag cccagtgccc cacctcggca atgcgccaat
     2581 cgactgggaa aaacaagcgc cgatttacaa ggatcgcgtg taccaggccc tcgagcgtca
     2641 catgcccgag ctcaggaagc acgtcgtcac cgagcgctgg ttttcgccgc aggacttcgt
     2701 caccgagctc aacgcattcc aaggctccgc gttctcggtc gcgccgaagc tgacccagag
     2761 cgcgtacttc cggccccaca atcgcgaccc gaacatcgcg gggctctatc tcgtcggagc
     2821 ggggacgcac ccgggagcag gactgccggg cgtgatcaac tcggccaagg cgaccgtcag
     2881 cgtcatcctg cgcgatttca gcgcggcgag cgacttggag cggaggacgg cgtgaacagc
     2941 gcgacgctcg cgttgccgca cgacacgagc gcggactgcg cggaggcgct gcgccagggt
     3001 tccaagagct tcgccttggc cggccgcttg ctgccgccgc aaatcgcgcg tgacgcagcc
     3061 acgctctaca cgttctgccg gcgtgtggac gacacagtag atctcgcagc gcccggtagc
     3121 gagcagcgcg cgctcgacgc ggtctggttg cgcgtccgct cggtattccg gggagagtcg
     3181 cagagcgacc cgttttgggc ctcgttcgcc gagctcgtgc gccgcgtgaa cctgcccgag
     3241 aggtacgtca tcgagctctt gctcggcatg cagatggacg tggacgggac gcgctacgag
     3301 accgtcgacc agctgctgct ctactgccac cgcgtggccg gcgtggtcgg gctcatgatg
     3361 tgccacgtgc tcggcgtgcg caccgacgct gctctgcgca acgctgcgca cctcggcatc
     3421 gcccttcaac tcaccaatat ctcgcgcgac gtacaggaag attgggagcg cggacggatg
     3481 tacgtccccg cggagctctt gaggaaggcc gggctccctc agctcgagcc agagccgcgg
     3541 gcgctgacgc gaacggagag caacgcattg tcgctggcgc tcgccgagct cgcgaagctt
     3601 gccgagcgct tttatcgttc gggagacgat ggcttgccgg ctctctcggc gcgagcgcgc
     3661 tttgcggtcc gcacggcgcg cttggtctac tcccacattg gagtgctgct ccagcgccgc
     3721 gactacgacg tgctcgccgg gcgcgtcatc gtccctgcct atcgcaagct cgttttgctc
     3781 ctgcgcgcgg ctctcgtcgc gctcgtcgaa tggcttttgc ccaggcgacg cgcggccgtg
     3841 aggctccccg agcgcgtact ccgctttccc gaagacgtct tacctgcgcc ctgaggtttt
     3901 agtcatgccc gcttcgagcc cgatcaaact ccgtgtgtcg aacgctcccg aactggctcg
     3961 gctccgtgag gcggagccgc ccccgcacac gtcgttcttg aaagaggtcg cgatttacgt
     4021 cggccgtgcg cgccaattca atcgcacgga ctggctcgtc tacgtgagct gggtcgggct
     4081 catcttcggt ctgttcggcg cgacgctcgg ttttctgggt gtcggtcacg cgcacggggt
     4141 gagctaccca gcagaggcgt ggctcttgcc gatcggcgcg ctgattttcg cgatcagcat
     4201 cgccgtcgat acgatcgggc accgcaccat ctacaaggag gagatcagcc gcgccgaggg
     4261 gctcgtgcac cacatcacga ttttctcggg aatcacgagc tgcgtgctgc tttgccttgc
     4321 atacacgtac ccgcgcgcgc tctgggtgcc ggccctggtg atgaccgcgc tctcgctact
     4381 ctatagcctg atcgacgagg cgttccactg gcgccgctac gtgcaaaagt tctcggatcg
     4441 cgtggagatg tggagccacg tcggcatcct cacgggccac accaccttga tgatcgcctg
     4501 gtggtggtgg ttcttcgacg gatatcgggg agtcgccgaa accctaccct tcttgctctg
     4561 atccgaggac gatccgtcct tgcgtcgaga gcgagtcagc cgaatcctgg caaggtccgc
     4621 tcggtccccc agcgctcgag gtatcgctcg aggtcgagct tccgtcgagc gctgtcgctc
     4681 gtcatgtacc gaatcatgcc gtagatgggc cgctcggggc cccacgggcg atcgtagcgg
     4741 ccaaagcacc agccaatccc gctgtaagag ttcg
//
LOCUS       DASTKS010001055         6023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1059823, whole genome shotgun sequence.
ACCESSION   DASTKS010001055 DASTKS010000000
VERSION     DASTKS010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059823"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFQ35_22265"
     CDS             <1..492
                     /locus_tag="VFQ35_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933453.1"
                     /translation="PYPNRMSYVRELRPYMYWLGYLSDGRAAGELDWQAIGGTWGTAT
                     SRVGRWEPGEGDHVPVRGFEALVRRVAERSPLLARYLHKYFADMARHCRDLYPALKSG
                     GKLHYVVGNSKFYDVMVPVERIFAELFEAAGFTKASARAIRKRSSKRELFEFLVSAEK
                     PSR"
     gene            589..3399
                     /locus_tag="VFQ35_22270"
     CDS             589..3399
                     /locus_tag="VFQ35_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HET9933454.1"
                     /translation="MTLLGDHPYRSFLSKVAKPTRYTGAEHGVRRKDWSSVAARVCLA
                     FPDIYDIGMSHLGFRILYKILNDDPRTLAERCYTPWVDMQKELLAHGKHLVSLESARP
                     LCDFDVLGFSLQYELTYTNILTMLDLGGVPLRSCQRSDDDPLVIAGGPVATHAEPMAP
                     FFDAILIGDGEEAATEIALSWVEGKRKRLSREERLLELAKIRGVYVPSLYRTELDADT
                     GFEVVVGGKHPDAPFPIERRLLDDLAKFPFPDDGPVGGPEAIFDRMSVEVARGCTEGC
                     RFCQAGMIYRPVRERSPEEIVRTVKSALEKSGQDEVSLTALSTADVSCISPLIKRLVE
                     ETAPERVSLSVASLRAYGLGEDLLDDMRKVRASGLTFAPEAGTQRMRDVINKNVTEEQ
                     LLDTAQRVFSRGFDKMKLYFIIGLPTEEDEDVLGIVSVGANALAVGKRVGKYPRVTVS
                     VSTHVPKPHTPFQWCAQDSLDEVRRKQGILRDAARRVKGLTLRLHDSSTSVLEGVLAR
                     GDRRLANVIEDVYLGGGRFDSWEDQLRSDLWQAALERHQIPIEAFLGTIPTNARLPWD
                     HFDIGLEEGFLLREYRKALAGRASPPCGKVAGSFIHATNVRDAEAETKRLVCYDCGVA
                     CDLGKMRSERIHFLRAMGAIEPGQRARLPVLNAPPKAPAKPIPQDSPEAFRPEREGLV
                     RRFRLRFEKTGAAALLGHLDLIRELPRAVRRAGARTAYTRGFHPKPDMTFSPALSLGV
                     ASLDEYLDIGLVEPPEPAELIARLERAASGGLRFLSAVPLEPKDPSVSAIVDAGRYLI
                     AFAEQALKPIGGRQGLEQRVQAFLDKAECVVRRDVDGIGKMVDVRRFTQSARLGDIGA
                     EQLLLDAGLRGALVPLEVTMRITQNGSAKIGEVVEAIFGEKAFPYQAVRVALLAGDKT
                     PLDVAAFRKPKPQTESIAAAPA"
     gene            complement(3482..4426)
                     /locus_tag="VFQ35_22275"
     CDS             complement(3482..4426)
                     /locus_tag="VFQ35_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933455.1"
                     /translation="MKSDQEFYRRVVSDTLDSIVSPGARDTLLTEALSLAGEIAVPRR
                     AREFRAFVRGPLSETLERALGQELGRSVAAELMRLSEAAPASSVPPPMAARHGARQRP
                     EPAASVPHSEAEHGPEHSGARRPRSNQVSTVPPAAARAKVANAPTLPARPRREAPPNE
                     ESQRARPPISNDYPAGVARAFGMISSNPVAAPNRRLPVVFLATRDAELVRRFSSWLDP
                     RASVVRVLRLADLLLHVEDAGERKMVIVLDGHSPPLRVEALAALCEELKLSVVLWGGT
                     RDVTARLTDLFPRVSEWLVCGDQTPLADVVDRCAQMVG"
     gene            4581..5621
                     /locus_tag="VFQ35_22280"
     CDS             4581..5621
                     /locus_tag="VFQ35_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HET9933456.1"
                     /translation="MNKIVVHRPGGFGQLKLETHASPEPADDEVRVSTRAVGVNYADC
                     IVRLGLYKSAKEFVGWPITPGFEFAGVVNAVGASVRSVSVGDPVFGVSLFGAYASELV
                     TKESFVRRMPAGLEMTQAATFPVAFLTAWYALRELARVSAGASVLVHSAAGGVGSALC
                     QLARANGVRCFGVVGSAHKVAHATAQGASAVVDKSREQLWERAREFAPKGFDAIFDAN
                     GADTLRESYAHLKPMGRLVIYGYHTMRSHGGIPNPFKLVWQFLRTPRFNPLDMVNRNV
                     AVMAFNLSYLFEHAPLYRTAMDELIGQLETGALTPLPVQEFPLARAADAHRQIQSGRT
                     LGKLALIPTDAP"
     gene            complement(5696..>6023)
                     /locus_tag="VFQ35_22285"
     CDS             complement(5696..>6023)
                     /locus_tag="VFQ35_22285"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HET9933457.1"
                     /translation="DQLFGNQGYSHNFHFTLATKLDFRYVGGEVFRFRGDDDLWVFIN
                     KKLAIDLGGLHQSASGEVALDVAASKLGLVRGQEYPLHLFFAERHTIDSNFSIETSIA
                     DPGSCE"
BASE COUNT         1039 a         2031 c         1950 g         1003 t
ORIGIN      
        1 ccctacccca accgcatgag ctacgtgcgc gagctccggc cgtacatgta ctggctcggt
       61 tatttgagtg acggtcgcgc cgccggggag ctcgattggc aggcaatcgg cggaacttgg
      121 gggacggcga cgagccgcgt agggcgctgg gagcccgggg agggcgatca cgtacccgtt
      181 cgcggcttcg aggcgttggt gcgtcgggtc gccgagcgga gcccgctcct cgcgcggtat
      241 ctgcacaagt actttgccga catggcgcgc cactgtcgtg acctctaccc agccctgaaa
      301 tccggcggga agctccacta cgtggtcgga aactccaagt tctacgacgt gatggtccca
      361 gtcgaacgca tcttcgccga gctcttcgaa gcggcgggct tcacgaaagc gagcgcgcgc
      421 gccattcgca agcgttcgtc caagcgtgag ctcttcgagt ttctcgtgtc ggcggagaag
      481 ccgtcgcgct gaacgcattc tcactggcgc cgacgcgcgg tacgcggcgc atcttttgcg
      541 cgtttccggt cgaccttggc cccctcaaag gtcagagtac ccgccccgat gacgctcctt
      601 ggcgaccatc cctaccgcag cttcctctcc aaagtcgcga agcccacgcg ctacaccggt
      661 gccgagcacg gcgtccgcag aaaggattgg tcgagcgtcg ccgcgcgcgt ctgcctcgcg
      721 ttccccgaca tctacgacat cgggatgagc cacctcgggt tccggatcct ctacaagatc
      781 ctgaacgacg acccgcgcac gctcgccgag cgttgttaca cgccgtgggt cgacatgcag
      841 aaggagctcc tcgctcacgg caagcacctg gtgtcgctcg agagcgcacg cccactctgc
      901 gacttcgacg tgctcggttt ttcgctgcag tacgagctca cgtacacgaa catcctcacg
      961 atgctcgatc tcggtggggt gccactccgg tcgtgccagc gcagcgatga cgatccgctg
     1021 gtgatcgcgg gcggtccggt cgcgacccac gccgagccga tggcgccgtt cttcgacgcc
     1081 atcttgatcg gcgacggaga agaagcggcg accgaaatcg cgctctcgtg ggtggagggc
     1141 aaacgcaagc gactctcgcg cgaggagcgc ctgctcgagc tcgcgaagat ccgcggggtc
     1201 tacgtgccga gcctctatcg caccgagctc gacgcggaca ccggtttcga ggtggtggtt
     1261 ggggggaagc acccggacgc gccgttcccc atcgagcggc gtttgctcga cgatctcgcg
     1321 aagtttccgt tccccgacga cggcccagtg ggcggccccg aagccatctt cgatcgcatg
     1381 agcgtagagg tagcgcgcgg ctgtaccgag ggctgccgct tctgccaagc ggggatgatt
     1441 tatcgcccgg tgcgcgagcg ctctcccgaa gagatcgtac gcacggtgaa gagcgcgctc
     1501 gaaaagtcgg ggcaagacga ggtgagcctc accgcgctct cgaccgcgga cgtgagctgc
     1561 atttctccgc tcatcaagcg cctcgtcgag gagacggccc ccgagcgcgt gagcctcagc
     1621 gtggcctcgc tgcgcgccta cgggctcggc gaagacttgc tcgacgacat gcgcaaagtt
     1681 cgcgcgagcg ggctcacttt cgcgcccgaa gccggcactc agcggatgcg cgacgtcatc
     1741 aacaagaacg tcaccgaaga gcagctgctc gacaccgcgc agcgcgtgtt ctcgcgcggc
     1801 ttcgacaaga tgaagctcta cttcatcatc ggcttgccca ccgaagagga cgaggacgtg
     1861 ctcggcatcg tcagcgtcgg cgcgaacgcg ctcgcggtcg ggaaacgcgt cgggaagtat
     1921 ccgcgcgtga cggtcagcgt ttcgacgcac gtcccgaagc cgcacactcc gttccagtgg
     1981 tgcgcgcagg attcgctcga cgaggtccgc agaaaacagg gcattttgcg ggacgcggcc
     2041 cggcgcgtga aaggcctcac gctgcggctc cacgattcga gcacgagcgt cctcgaaggc
     2101 gtgctcgcgc gcggcgaccg gcgcctcgcc aacgtcatcg aagacgtgta cctcggcggc
     2161 ggccgcttcg actcctggga agaccagctg cggagcgatc tgtggcaagc ggcgctcgag
     2221 cggcaccaaa tcccaatcga ggccttcctc ggcaccatcc cgacgaacgc gcggctaccc
     2281 tgggatcact tcgacatcgg cctcgaagaa ggcttcttgc tccgcgaata ccgaaaggcg
     2341 ctcgccggcc gcgcgagccc gccgtgcggc aaagtcgcag gttcgttcat tcacgcgacc
     2401 aacgttcgcg atgccgaagc ggagaccaag cgcctcgtct gttacgactg cggggtcgcc
     2461 tgcgatctcg gaaagatgcg cagcgagcgc atccatttct tgcgcgcgat gggcgccatc
     2521 gaacctgggc aacgcgcgcg cctgccggtg ctgaacgcgc cgcccaaagc gcccgccaag
     2581 cccattcccc aagactcccc cgaggcgttc cgtcccgagc gcgaaggctt ggtccggcgc
     2641 ttccgcctgc gcttcgagaa gaccggagcc gcagcgctgc tcggtcacct cgacttgatt
     2701 cgtgagctgc cgcgcgccgt tcgccgtgcg ggagcgcgca ccgcgtatac ccgaggcttc
     2761 catccgaagc ccgacatgac cttcagcccc gcgctctcgc tcggcgtcgc gagcctcgac
     2821 gaatatctcg acatcggctt ggtggaaccg ccggagcccg ccgagctcat cgcgcgcctg
     2881 gagcgcgcag cgagcggggg attgcgcttt ctcagcgctg tcccgctcga gccaaaagat
     2941 ccgtccgtca gcgccatcgt cgacgccggc cgctatctga tcgcgtttgc agagcaagcc
     3001 ctgaagccaa tcggcggccg ccaaggcctc gagcagcgcg tccaggcgtt cctcgacaag
     3061 gcggagtgcg tcgtccgccg cgacgtcgat ggcatcggca agatggtcga cgtgcgtcgc
     3121 ttcactcagt cggcgaggct cggcgatatc ggcgcggaac agctgttact cgacgccggc
     3181 ctgcgcggcg cgctcgtccc cctcgaggtg acgatgcgca tcacgcaaaa cggctccgcg
     3241 aaaattggcg aggtcgtgga agcgattttc ggcgagaagg cgtttccata tcaggccgtg
     3301 cgcgtcgcgt tgttggcggg ggacaagacc ccgctcgacg tcgcggcatt tcgaaagcca
     3361 aagccgcaaa ccgagagcat cgccgccgcg cccgcctgag caacgcgcga cgaacgagta
     3421 cggctctcgg gtgcggccgt gaagcacccg cgcggaacgg cgacgagagc gtcgcgctgg
     3481 ctcagccgac catctgcgcg cagcgatcga ccacgtccgc gagcggcgtc tggtcaccac
     3541 acacgagcca ctccgagacg cgcgggaaca agtcggtgag tcgcgcggtg acgtcacgag
     3601 tgcctcccca aagcacgacg ctgagcttca gctcctcgca aagcgcagcc agcgcctcga
     3661 cgcgcagagg aggactgtgg ccatcgagca cgatgaccat cttgcgctcg cccgcgtcct
     3721 cgacgtgaag gagcaggtcc gcgagccgca acacgcgcac caccgaggcg cgtggatcga
     3781 gccaggagga gaagcgccga acgagctccg cgtcgcgcgt cgccaggaag actaccggca
     3841 atctgcggtt cggggccgcg accgggttcg acgaaatcat gccgaaggcg cgcgctacac
     3901 cggcggggta gtcgttggag atcggcggtc gggcgcgctg gctttcctcg ttgggcggcg
     3961 cttcgcgacg cggtcgcgct ggaagcgtcg gggcgtttgc gaccttcgca cgagctgctg
     4021 ccggcggaac cgtcgacacc tgattcgagc gcggtcgccg tgcgccggag tgctcgggtc
     4081 catgctctgc ttcgctgtgc ggaaccgaag cggcaggctc cgggcgttgc cgcgcgccgt
     4141 gtctggctgc catcgggggc ggcacgctcg acgcaggagc cgcttcgctc aggcgcatga
     4201 gctcggcggc caccgagcga ccgagctctt gtccgagcgc ccgctcgagg gtttcgctga
     4261 gaggaccacg cacgaacgcg cgaaactccc gtgctcgccg cggaacggcg atttcgccag
     4321 cgaggctcag cgcctcggtc agtaacgtgt cgcgcgcccc ggggctcacg atgctgtcga
     4381 gcgtgtcgct gaccacacgc cgataaaact cctgatctga tttcacactg cctcgcgcaa
     4441 gcccctctat tcggctcagg ttactccgca tgagacacgc tggccactcg gcatgatgaa
     4501 gtgcgtcacg acatggtaag taactttccc aggctgtctc actcacaccg cgccacctca
     4561 acgactcgat gtttcgtccc atgaacaaga ttgtcgtgca ccgccccggc ggcttcggtc
     4621 agttgaagct cgaaactcac gcgtcgcccg aacccgcgga cgacgaggtg cgcgtcagca
     4681 cgcgcgcggt cggcgtgaac tacgcggact gcatcgtgcg cttggggctc tataaatcgg
     4741 caaaagagtt cgtcggttgg cccatcactc ccgggttcga gttcgcgggt gtggtgaacg
     4801 cggtgggggc cagcgtccgc tccgtttcgg taggtgatcc cgtgttcggc gtgtcgttgt
     4861 tcggcgctta tgcaagcgag ctcgtgacca aggagagctt cgtgcggcgc atgccggccg
     4921 gcctcgagat gacgcaagct gccaccttcc cggtcgcctt tctcaccgct tggtatgcgc
     4981 tgcgagagct cgcgcgcgtc tcggcgggcg cgtcggtgct ggttcactcc gcggccggcg
     5041 gcgtcggcag cgcgctctgc cagctcgccc gagcgaacgg cgtgcgctgt ttcggcgtcg
     5101 tcggcagcgc gcacaaggtc gcgcacgcga cggcgcaagg agcgagcgcc gtggtcgaca
     5161 aaagccgcga gcaactgtgg gaacgcgcgc gcgaattcgc tcccaaaggc ttcgacgcaa
     5221 tcttcgacgc gaacggcgcc gacacgctgc gggagagcta cgcgcacctg aagcccatgg
     5281 ggcggctcgt gatctacggc tatcacacca tgaggtcgca cggcggtatt ccgaacccgt
     5341 tcaagctcgt ttggcagttc ttacgcacac cgcgtttcaa cccgctcgac atggtgaacc
     5401 gaaacgtcgc ggtgatggcc ttcaacctct cgtacctctt cgagcatgcc ccgctctatc
     5461 gcaccgcgat ggacgagctc atcgggcagc tcgaaaccgg cgcgctcacg ccgctccccg
     5521 tccaagaatt ccctttggcc cgcgccgccg acgcgcaccg ccaaattcag tccggaagaa
     5581 ccctcggaaa gctcgcgctg atcccgaccg acgcacccta gaacgccgat cagtctgact
     5641 tctcgctgtt ctagggggga aggcacgcgc cggcctcttc cgaagtgagt tgcccttact
     5701 cgcagcttcc aggatccgcg atcgaagtct cgatcgagaa gttcgagtcg atcgtgtgtc
     5761 gctccgcgaa gaacaggtgc agtggatact cttgcccgcg cacgagtccg agcttcgacg
     5821 cagcaacgtc caaggcgact tcgcccgacg cgctctggtg taaaccaccg aggtcaatcg
     5881 cgagcttctt gttgatgaag acccagaggt cgtcgtcgcc gcggaagcgg aacacctcgc
     5941 cgccgacgta acgaaagtcg agcttcgtcg cgagcgtgaa gtggaagttg tgggagtagc
     6001 cctggttgcc gaagagctgg tcg
//
LOCUS       DASTKS010001056         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_389171, whole genome shotgun sequence.
ACCESSION   DASTKS010001056 DASTKS010000000
VERSION     DASTKS010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389171"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2566
                     /locus_tag="VFQ35_22290"
     CDS             <1..>2566
                     /locus_tag="VFQ35_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9933458.1"
                     /translation="LRTTFTFGAAGDAEICIHEEPRIALKEKSWSAAEGDFGAWVTRQ
                     AVADAQSPFELGTGPLSRATLLSRGAREHVLLFTIHHAVWDGWSAGVLGQELVSLYGA
                     LSAGKPSPLAKLETQYADYAAWQRQRLQGEVLEQKVAFWKSELADAPAAIDLPTDRPR
                     PRIQSYAGKTLFTKLGSPLSARVDACAKELGRTPFAVLFAAYAVLLARHSGQRDLVIA
                     TPTANRPHSELEALIGCFVNTLALRVELGRDLKFIELVHRVSERLLRTAPHQDLPFEK
                     LVEALAVERDMSRSPVAQTMFVLQGTPRSALRVGELTFTPIDVDAGFAKLDLTLNVCP
                     SGQGYEAAWEYNTDLFDDETALRMSAQYERLLESFVGSPNSEVWRAALLSEAEQNQLL
                     YDRNRTNLEVPSESTLHELVLARARGAPDARALVVGSAAWTYGELLGRAESVASELSR
                     RGARVGDLVAVFLRRDEHLVPALLGVLLVGAAYVPLDPAYPEDRIGLILEDTNAKLIV
                     TTGELRERIGGEFQDRVLDVTLLPARPEESAPLAVGGQAADIAYVIYTSGSTGRPKGV
                     AIEHRNAVAFIAWALTVFQRSELEGVLAATSVCFDLSVFELFGTLSAGGTVILAENAL
                     ALPELPARDEVTLINTVPSAIVELVRAEAIPRSVRTVNLAGEALPEETVVRLYQLPHV
                     ERVYNLYGPSETTTYSTYTLTTRGAWRPTIGRPIANTRVYVLDEHLAPVPLGVRGELY
                     IAGAGVARGYVARPELTAERFLPDPFARRGRMYKTGDVVRWLPTGELEYLGRNDHQVK
                     LRGFRVELGEIEATLRSLPHVESAVVVVREDRPGNKQLVGYVVAEATQALSVEGLRA"
BASE COUNT          418 a          857 c          839 g          452 t
ORIGIN      
        1 gttgcgcacg acgttcacct tcggcgccgc aggcgacgcc gagatctgca tccacgaaga
       61 gccgcgcatc gcgctaaagg aaaagtcttg gagtgcagcc gagggcgatt tcggcgcttg
      121 ggtcacgcgc caggcggtcg ccgacgcaca gagccccttc gagctcggca ccggccctct
      181 gtcgcgggcg acgctcttgt cgcgcggcgc tcgcgagcac gtcctgctgt tcacgatcca
      241 tcacgccgtg tgggacggat ggtccgcggg cgtgctcggc caggagctcg tcagcctgta
      301 cggcgcgctc tctgcgggaa agccgtcgcc gctcgcgaaa ttggagactc agtacgccga
      361 ctacgccgcc tggcaacgtc agcgcctgca gggggaagtc ctcgagcaaa aggtcgcgtt
      421 ctggaagagt gagctcgccg acgctcctgc cgcgatcgac ctgcccacgg atcgacctcg
      481 tccccgcatc caaagctacg ccggcaagac gctcttcacc aagttgggga gcccgctatc
      541 cgcccgcgtc gacgcctgcg ccaaggagct cggccggacg cccttcgcgg tcttgttcgc
      601 tgcgtacgcg gtcttgctcg cgcgccatag cggccagcgg gatctcgtca tcgcgactcc
      661 tacggcgaat cggccgcaca gcgaactcga ggcgctgatc ggctgtttcg tgaacacgtt
      721 ggcgcttcgg gtcgagctcg gccgcgatct gaagttcatc gagctcgtcc atcgcgtgag
      781 cgagcgcttg ctgcgcactg ctcctcacca agacctgccg ttcgagaagc tcgtcgaagc
      841 gctcgcggtg gagcgcgaca tgagtcgcag ccccgtcgct caaaccatgt tcgtattgca
      901 gggaacaccg cggagcgcgc tgcgggttgg agagctcacc ttcacgccga tcgacgtcga
      961 tgccggcttc gcgaagctcg acctcacgct gaacgtgtgc cccagtggtc agggatacga
     1021 agcagcgtgg gagtacaaca ccgacctgtt cgacgacgaa accgccttgc ggatgagcgc
     1081 gcagtacgag cggctactcg aatccttcgt tgggtcgcca aattcggagg tgtggcgcgc
     1141 cgctttgctg agcgaggccg aacagaacca gctcctctac gatcgcaatc ggacgaacct
     1201 cgaagttcct tcggagtcca cgctgcacga gctcgtcttg gctcgagcgc gcggagcgcc
     1261 ggacgcgcgc gcgttggtcg tcggaagcgc ggcgtggacc tatggcgagc tgctcgggcg
     1321 cgcggagagc gtcgcgagtg agctctctcg acgcggagcg cgcgtgggcg atctcgtcgc
     1381 tgtattcttg cgacgagacg agcacttggt ccctgcgctt ctcggcgtgt tgctggtggg
     1441 cgccgcctac gtgccgctcg accctgccta tcccgaggat cgcatcggcc tcatcctcga
     1501 agatacgaac gcgaagctca tcgtcacgac cggtgagctt cgggagcgca tcgggggcga
     1561 gtttcaggac cgcgtgctcg acgtgacgct tctgccggcc cggcccgagg aaagcgcgcc
     1621 gctcgcggtc ggaggccagg cggcggacat cgcgtacgtg atttacactt cgggctccac
     1681 gggtcggccc aagggcgtcg cgatcgaaca tcggaacgcc gtcgccttca tcgcgtgggc
     1741 actcaccgtt tttcagagaa gcgagctcga aggtgtgctg gcggcaacct cggtgtgctt
     1801 cgacctctcg gttttcgagc tcttcggcac gttgagcgcg ggtggcaccg tcatcctggc
     1861 cgagaacgcg ctcgcgctac ccgagctgcc tgcgcgcgac gaagtcaccc tgatcaacac
     1921 ggtgccgtcg gccatcgtcg agctcgttcg cgcggaggcg attcctcgca gcgttcgcac
     1981 cgtaaacctc gcgggcgaag cgctgcccga agagacggtc gtccgccttt accaacttcc
     2041 ccacgtcgag cgagtctata acctctatgg cccgtccgag accacgacgt actcgaccta
     2101 cactctgacg acgcggggcg cctggcggcc gaccatcggg cgccccatcg cgaacacacg
     2161 cgtttacgtc ctcgacgagc atttggcgcc ggtgccgctc ggcgtgcgcg gcgagctcta
     2221 catcgccggc gccggggtag ctcgcggcta cgtcgcgcgg cccgaactca cggccgagcg
     2281 cttccttccc gacccctttg ctcgacgcgg tcgaatgtac aagaccggcg atgtcgttcg
     2341 ctggctgccc acaggggagc tcgagtacct cgggcgaaac gaccaccagg tgaagcttcg
     2401 gggctttcgc gtcgagctcg gcgaaatcga ggcgactctg cgttcgttgc ctcacgtcga
     2461 atccgcggtg gtcgtcgtgc gtgaagaccg tccagggaac aagcagctcg ttgggtacgt
     2521 cgtcgccgaa gcgacgcagg ccctatcggt agaaggcttg agagct
//
LOCUS       DASTKS010001057         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1730064, whole genome shotgun sequence.
ACCESSION   DASTKS010001057 DASTKS010000000
VERSION     DASTKS010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730064"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFQ35_22295"
     CDS             <1..218
                     /locus_tag="VFQ35_22295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933459.1"
                     /translation="TLKLFVERHPEHSGGTTTTISSNEVRSFSMSQGDMLWLVTDSGK
                     PLSSLSASGGPFVDMVILESCSGFAPY"
     gene            905..1081
                     /locus_tag="VFQ35_22300"
     CDS             905..1081
                     /locus_tag="VFQ35_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933460.1"
                     /translation="MEKKKDKARVVIDIEDLREITAQTEDAATGEQSDEASVKVRGAR
                     AAAEPIKSTIMCPW"
     gene            1168..2643
                     /locus_tag="VFQ35_22305"
     CDS             1168..2643
                     /locus_tag="VFQ35_22305"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9933461.1"
                     /translation="MTAVKRLSDHIKLQLIYQDETPFGSLLSGRIDDFVRAACSEIAA
                     LRRERGLNHVVKHSCDLLARARRELGGLYESRERLREDCLYPPARSVRAMGLVVESAE
                     GELATIELAPRLIANLASWLGEWQSGASRPSTGAARALWDALDSVGALTEAPALSRVP
                     GDVLFGGHAMVRIGDAGEAALFDPFLLPWSRSYPAEYQPVAASELAPKLICVSHSHPD
                     HFDLGSLLRFGADTPIYVPHVPRESILAVDMASRLREVGFTNVRALDWGESVAVEGLS
                     VEALPFYGEQPTTSDVLHPDVRNFGNAYRVDGVVGRYALTVDCGADLSGTTLQLASRS
                     KQDRGPIDALFAGYRAWALYPIRYLFSSVARYLLFVPEPLWAVRQKIMDDANDALDAA
                     ERWGARFLVPYADGGAPWYWQRGLGPRLDAPPAQRELHFDPPPEHVQEVARARSAGDE
                     SFVASPVHVEVARPGDALTLESDRLRRGRVDGHRWPYAEVC"
     gene            2721..3059
                     /locus_tag="VFQ35_22310"
     CDS             2721..3059
                     /locus_tag="VFQ35_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933462.1"
                     /translation="MEEEPLPVTRKKALLRILSAAAAKQRALVPSASEFADFSAAFRR
                     AFAIDDEQRLQRWLNHSRLDRKDFERMLLELCLQSKLEEHLATHIDREVTLQQAIWTL
                     HSWAHAERAT"
     gene            3056..>3437
                     /locus_tag="VFQ35_22315"
     CDS             3056..>3437
                     /locus_tag="VFQ35_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933463.1"
                     /translation="MNASLGPLAIVRGASWPLETLEDFACSELTELATARADTEEYRR
                     AYEAALQRNRETLWAATQRSVRFMVALTVSNPELARRTSECDWSRRNKRVRHLETTLF
                     RHLSRAVGRTDPCDLWTGVALAAWA"
BASE COUNT          625 a         1085 c         1126 g          601 t
ORIGIN      
        1 gaacgctcaa gttgttcgtg gaaagacatc cggagcacag tgggggcacg actacgacga
       61 tctcttccaa cgaggtccgg agcttctcga tgagtcaggg agatatgctt tggctggtga
      121 ccgattcggg gaagccgctg agcagcctga gcgcgagcgg cggcccgttc gtggacatgg
      181 tcatcctcga gtcctgcagc ggctttgctc cctactgacg cccgcggcgc ctgagccacc
      241 cgcagtgagt gcgcgttggc agcgcgtgga attcaagtcc ggtcaccgtg ctccgtgatg
      301 cgcggcgatc gcgaacgcac tgcgggggcg gaagtgagtg ctcgacgtcg taggaccacg
      361 aagtcgagat cagcgctgca agctcgaagc gcgtgacaag cgcacgaacg tcgacaccgc
      421 gaagctccgc gagcaacgcg ctgacctcac gggccgggcc atccacagcg cggcgtcgac
      481 gattcgaagg gaaagctgcc atcgagcgga gcctctccgc ctggcgtccg cgccgggtag
      541 cgcgaacgga acgagagcga gccgaactgc gtcaccgatt gcatccggtt gcatcgaacg
      601 ccagcgccgc gccctagaat cggagccatc tcacatgcgc tgcggaagcg gccgcgagga
      661 agttcctatg tcgcgacggg ttcgtgacgt gatcgaacca cgcaaacagc gctgcgcctc
      721 gtgcgcgcga aaccgatcgt gaaagaactc acgcttcgct aggagaacgt ttgatgcacg
      781 agtgccatcg gctgcaaacg atctccgaga gtcaatgact attggatgaa cgacgtggag
      841 cagttaggtt cactcgagtc ttgcgaggcc actcggcacg caaaaacgga agaggagagt
      901 cctaatggag aagaagaaag acaaggcacg cgtcgtcatc gacatcgaag atttgcgcga
      961 aatcaccgcg caaacagagg acgcggccac cggcgagcag tcggacgagg cttctgtcaa
     1021 ggtgaggggc gcacgcgccg ccgcggagcc gatcaagtcc acgattatgt gtccttggtg
     1081 aggtagagag gttggcgcgc tctcgggtgc gccaacctcg cttttgcgag tccgagcaaa
     1141 gctcgcgagc acgacgagag gcttacggtg accgcggtga aaaggctgag cgaccacatc
     1201 aagctccaac tcatctacca ggacgagacg ccgttcggca gcttgctctc gggacgcatc
     1261 gacgacttcg tccgcgcggc atgctccgaa atcgccgcgc ttcggcgcga gcggggactg
     1321 aaccatgtgg tgaagcactc gtgtgacctg ctggcgcgcg cgcgccgcga gctcggcggt
     1381 ctctacgaaa gccgagagcg cttacgggag gactgcctct accctccggc tcggagtgtt
     1441 cgcgcgatgg gcctcgtcgt cgaatcggcc gaaggtgaac tcgcgaccat agagcttgcc
     1501 ccgcggctga tcgccaatct cgcgagttgg ctcggcgagt ggcagtcggg ggcctcgcga
     1561 ccgtccaccg gcgcggctcg cgcgctatgg gatgccctcg attcggtcgg agcgttgacg
     1621 gaggcgcccg ccctgagccg agtacccggc gacgtgctct tcgggggcca cgccatggtg
     1681 cgcatcggcg acgcgggcga agcagccctg ttcgatccct ttctcttgcc ttggtctcgg
     1741 tcgtaccccg ccgaatatca accggttgcg gcgagcgaac tcgcaccgaa actcatctgc
     1801 gtcagccact ctcaccccga ccatttcgat ctcggttcgc tcttgcgttt tggagccgac
     1861 acacccattt acgtgccgca cgtaccgcga gagtcgatat tggccgtgga catggcctcg
     1921 cgccttcggg aggtagggtt cacgaacgtg cgcgctctcg attggggaga gagcgtcgcc
     1981 gtcgaggggc tcagcgtcga ggcgctcccg ttttacggcg aacagcccac cacgagtgac
     2041 gtcctgcatc ccgacgtgag aaatttcggc aacgcttatc gcgtcgacgg agtggtcgga
     2101 cgctatgctt tgaccgtcga ctgcggcgcg gacctgagcg gcaccactct gcaacttgcc
     2161 tcgcgcagta agcaagaccg gggtcccatc gacgcgctct tcgctggata tcgcgcctgg
     2221 gcgctctatc ccattcggta tctcttcagc tccgtcgcgc gctatctctt gttcgtgccc
     2281 gagccgctct gggcggtgcg gcaaaagata atggacgacg caaacgacgc gctcgacgcg
     2341 gcggagcgct ggggagctcg gttcctggtt ccctacgcgg acggtggcgc cccttggtac
     2401 tggcagcgcg ggctcggtcc ccgtctcgat gcaccaccgg cgcaacgcga gctgcacttc
     2461 gatccgccac ccgagcacgt gcaagaggtc gcccgggcgc gctccgctgg cgacgaatct
     2521 ttcgtcgcgt ccccggttca tgtcgaagtg gcgcggccgg gagatgcgct cactctcgag
     2581 agcgatcgtc tccgccgcgg tcgagtggac ggccatcgct ggccttacgc ggaggtctgc
     2641 tgatgacgct ctcggcccga gcgtgcgatg aggattcgac taatcgacga ccgttcccca
     2701 acgatgacgg gcggtcgaac atggaagagg agccgttgcc cgtcacccgc aagaaggctc
     2761 tcctgcgtat tctttctgcc gcggctgcga agcaacgggc tctcgtcccc tccgcttccg
     2821 aattcgccga cttttccgcg gcgttcaggc gcgccttcgc aatcgacgat gaacagcggc
     2881 tgcagcgttg gttgaaccat agccgcctcg atcgaaagga tttcgaacga atgctgctcg
     2941 agctttgcct tcagtccaag ctggaagagc acctggcgac tcacatcgac cgcgaggtca
     3001 cgctgcagca ggcgatctgg acgttgcact cctgggctca cgcggagcgc gcgacatgaa
     3061 cgcgtctttg gggccgctcg cgatcgtgcg gggtgccagt tggccgctcg agacattgga
     3121 ggacttcgct tgcagcgagc ttaccgagct tgccacggcg cgcgccgata ccgaggaata
     3181 tcggcgcgcc tacgaggcgg cgctgcagcg aaaccgcgag accttgtggg cggccacgca
     3241 gcgaagtgtg cggttcatgg tagcgctcac cgtgtcgaac ccagagctcg cccgtcgtac
     3301 cagcgagtgc gactggtcac gacggaacaa gcgcgtgcgc cacttggaaa cgacgctgtt
     3361 caggcacctg tcgcgcgcag tgggccggac ggatccgtgc gatctatgga ccggcgtcgc
     3421 gctcgcggcc tgggcag
//
LOCUS       DASTKS010001058         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_613061, whole genome shotgun sequence.
ACCESSION   DASTKS010001058 DASTKS010000000
VERSION     DASTKS010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613061"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFQ35_22320"
     CDS             complement(<1..904)
                     /locus_tag="VFQ35_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933464.1"
                     /translation="MSRARTGMRSFTLLLLPLSLTFAGQARAEPPELGTAPPARDGVK
                     PSPAKEAASCRLDKRLTFAADFAPYVGTSSSEVGRTAMRNFSFNLVGGYAGGLRGFEL
                     GSVLNLERACVEGVQVAGVLNASGGEVRGLQLSGAVNLTHGEVRGLQVAGAANVTRDA
                     QALQLSGAANVVIGAVEGAQLSGGVNLATGRLRGLQLSGGANVVTAQLSGAQIAGGVN
                     LGAEDVSGLQLAPVNVAAGNVHGVQLGVVNVAEKSDLSLGVVSINTRGRTQVTGWSAV
                     ETGFFAAAVKHGGNHWYGLYGLGTR"
     gene            1097..1654
                     /locus_tag="VFQ35_22325"
     CDS             1097..1654
                     /locus_tag="VFQ35_22325"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HET9933465.1"
                     /translation="MSARRLTSKSKLLSYVLRHGPESFGLVLERGGWVNVDTLLDALA
                     KHAKPVQRDELIRIVQDDEKQRFAISSDGSRIRANQGHSIEVELDFTPMPPPALLYHG
                     TIERFWPVIERDGLLRGARHHVHLSPSVELAEQVGARRGPPLVLEIRARDMTRDGYVF
                     YRSANGVWLTEHVPAAYLLRRAQRR"
     gene            complement(1602..2150)
                     /locus_tag="VFQ35_22330"
     CDS             complement(1602..2150)
                     /locus_tag="VFQ35_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933466.1"
                     /translation="MKNRVNRVLGVDVGRVIICPVSADGVEDTSFLSGTEQRALETPP
                     CDGAFEAIRDLVQLFEGRVWLVSKCGPRIQALTRRWLAHQRFHEFTGVPPTHVRFCLR
                     RPEKRGHCESIGATHFIDDRLDVLQHLRGAVPNLYWFGHQRTGAASPGWVTPTADFRI
                     ARERIFSDVARDAEGKPRARAR"
     gene            2220..>2552
                     /locus_tag="VFQ35_22335"
     CDS             2220..>2552
                     /locus_tag="VFQ35_22335"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9933467.1"
                     /translation="MINSDWLESFVSFAEDMNFTRAAERRHISQPALHVQIQKLSAQL
                     GAPLYVRRGRVLELTAQGREVLAFGREQRARSAQLEAALRPGAREQTVTLAAGEGTFL
                     HLLPPALRA"
BASE COUNT          451 a          899 c          763 g          439 t
ORIGIN      
        1 cgcgcgtccc caggccgtag agaccatacc aatgatttcc gccgtgcttc acggccgcag
       61 caaagaaccc ggtttcgact gccgaccagc cagtcacttg ggtgcgaccg cgcgtgttga
      121 tgctcaccac gcccaaagaa agatcgctct tttcggcgac gtttacgacc ccgagctgca
      181 cgccgtgcac gttgccagcc gcgacgttca ccggcgcaag ctgcaagccg ctcacgtctt
      241 ctgcccccag gttcacgccg cccgcgattt gtgcgccgct gagctgcgcc gtcaccacgt
      301 tggcaccccc gctcagctgt aaaccgcgca ggcgccccgt cgccaagttg acgccaccgc
      361 tgagctgcgc tccctcgacc gcgccgatca cgacgttggc ggcaccgctg agctgaagcg
      421 cctgcgcgtc gcgcgtgacg ttggccgctc ccgccacttg caagcctcgc acctcaccgt
      481 gcgtcaggtt gactgcgccg ctcaactgca agcctcgcac ctcgccgcca gacgcattca
      541 acactcccgc gacttgcacg ccttcgacgc acgcgcgctc caagttcaag acgctgccga
      601 gctcgaagcc gcggagccca cccgcgtagc cgccgacgag gttgaaggaa aagttcctca
      661 tcgcggtgcg gcccacctcg ctgctgctcg ttccgacata cggagcgaaa tccgcggcga
      721 aggtcaggcg cttgtcgagc cgacacgacg ctgcctcttt ggcggggctt ggcttcacgc
      781 catcgcgcgc tggcggcgcc gtcccgagct ccggaggttc cgcgcgagcc tggccagcga
      841 acgtgagact caaaggcaga aggaggagag tgaacgagcg cataccggtg cgtgcccggc
      901 tcaccgtggt ccgttcaacg aaatcctcga aatctcgaag tccgtcattt cgccactttg
      961 ctttcgtgct cgcgcccgac cggaagccgc cgcgccctct tgggtatccc gagttctccg
     1021 cccgactgcg agcccagctc ctgcggctgc gaaggctctc gcgaaggcta aaaacttggg
     1081 atacccctag ccctcgttgt cggcacgccg tcttacctcc aaatcgaagc tcttgagcta
     1141 tgtgctccgt cacggcccgg agtcgttcgg gctcgtactc gagcgcggcg gttgggtgaa
     1201 cgtggacacg ctgctcgacg cgctcgccaa gcacgcaaag cccgtgcagc gcgacgaact
     1261 cattcgcatc gtgcaagatg acgaaaagca gcgcttcgcc atttcctctg atggctctcg
     1321 cattcgcgcc aaccagggtc actcgattga agtcgagctc gacttcacgc cgatgccgcc
     1381 gccagcgctg ctctaccacg gcaccatcga acgcttttgg ccggtcatcg agcgcgatgg
     1441 tttgctccgc ggcgcgcggc accacgtgca cctgtcaccc agcgtcgagc tcgcggagca
     1501 ggtgggcgca cgccgtggac cgccgctcgt gctcgaaatt cgcgcacgcg acatgacgcg
     1561 tgacggctac gtcttctacc gttcggcgaa cggcgtctgg ctcaccgagc acgtgcccgc
     1621 ggcttacctt ctgcgtcgcg cgcaacgtcg ctgaagatgc gctcgcgcgc gatgcgaaag
     1681 tccgccgtgg gtgtcaccca acccggactt gccgccccgg ttcgttgatg cccgaaccag
     1741 tacaggttcg gcaccgcgcc gcgcaaatgc tggagcacgt cgaggcgatc gtcgatgaag
     1801 tgcgtcgcgc cgatggactc gcagtgaccg cgcttctccg gtcgacgcag gcaaaagcgt
     1861 acgtgtgtcg gagggacgcc ggtaaactcg tgaaaacgct gatgcgcgag ccagcgccgc
     1921 gtgagcgcct ggattcgcgg gccgcacttg gagacgagcc agactcgccc ctcgaacagc
     1981 tggaccagat cgcggatggc ctcgaacgcg ccgtcgcagg gaggcgtctc gagcgcgcgc
     2041 tgctcggtac ccgagagaaa tgacgtatct tcgacgccgt ctgcgctcac gggacaaatg
     2101 atgactcggc cgacgtcgac gccgaggaca cggttcactc tgtttttcat gaacgcgagg
     2161 gtgcccgacg cgtcaatctc gcagaactat tacgtattga tggtctataa ccaaatccta
     2221 tgatcaactc cgactggctc gaatcgttcg tctccttcgc agaggacatg aacttcaccc
     2281 gcgccgcgga gcgacgacac atctcgcagc ccgcgctcca cgtgcagatc caaaagctca
     2341 gcgcccagct cggcgcgcca ctttacgtgc gccgaggtcg agtgctcgaa ttgacggccc
     2401 agggccgcga agttctagcc ttcggaagag aacagcgtgc ccgaagcgcc cagttggaag
     2461 cggctctgcg ccccggggcg cgagagcaaa cggtgacgct ggcggccgga gagggcacct
     2521 tcctccactt gcttcccccg gctctccgcg cc
//
LOCUS       DASTKS010001059         5084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1508615, whole genome shotgun sequence.
ACCESSION   DASTKS010001059 DASTKS010000000
VERSION     DASTKS010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508615"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1804
                     /locus_tag="VFQ35_22340"
     CDS             371..1804
                     /locus_tag="VFQ35_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933468.1"
                     /translation="MLELLGDEVESYTAATAHDLDHAHLAPVHEVFTHEGVALLVFDK
                     IDGILLTKRLAEIGKKPELEAVGAALRVSDALSNLHDAGGVHGFIHTDSVLVDPPERL
                     GPVLTFAPIPSDERTFHSPERGETGKPSVADDAWAAAGLLHQMLTSKPPPKSGYSTVE
                     ELEEAGVENLALREALLHGLNSDPEKRSKDVRPLKRELARWFVEHAGGEAVLLGPHST
                     MPPPLPAGAKGLAQGVPQTAVEVGPRPRAKKQPRFVVLALAGTSLGLIAVWAYSMMHP
                     KPVPPAPVAAAATPAASEPPSTAAKAIDLSEVPVTGEERSIELGKTGSCVASYLPKGA
                     FSKEPDLGSLCSSTDPNKGAQDLRVALVNSAPKGAVTDAMKLWSRLGWYDMAAYAVVR
                     GGCCEEPKDLTLAEPAKGCDAIAEALRDLSKDVIGGKPYEESLKKYTGAIHCELNAGH
                     TQVPGRTQRPQPGEDTAFKDFVAAIQH"
     gene            2041..3027
                     /locus_tag="VFQ35_22345"
     CDS             2041..3027
                     /locus_tag="VFQ35_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9933469.1"
                     /translation="MQDVRALNDLVAKESAFIDDLTSEIGKVIVGQSYMIERVLVGLL
                     AGGHVLLEGVPGLAKTLTVRTICDAIHAKFSRIQFTPDLLPADLIGTVIYDQKTGEFA
                     SKLGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGDRSYPLPDPFVVMATQNPI
                     EQEGTYPLPEAQVDRFMLMIKVNYPTREEERAVMDRMTGPLTASASPRTSPERLLEAR
                     KVVRDVYVDDRVKDYIVDVVFATREPSAKGMKELGPLIEYGASPRASIALNLAARAHA
                     FLRHRGYVTPEDVKAIGPDVLRHRIVLSYEAEAEEVTAEDIVRRVFEVVEVP"
     gene            3065..3946
                     /locus_tag="VFQ35_22350"
     CDS             3065..3946
                     /locus_tag="VFQ35_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9933470.1"
                     /translation="MIPKELIKALRKIEITTNRLATEQLSGNYTSVFRGQGLAFREVR
                     EYQPGDDVRTIDWNVSARMNNTYVKVFVEEREMTVMLVVDLSQSQQFGTQRGSKAALA
                     AEVSALCAFSAIKHNDRVGLILSTDQIEKIVPPLKGQKHVMRVVREILGAKPERPGTD
                     LKIALETLYRVARRRSVAFVLSDFFATDYERALSLAAARHDVIPVMLVDPRDDELPDV
                     GLAAFEDLETGESVLVDTGSKSVRAHYARRMREQRETQIKLFRKLGLDHVVVRTDRPY
                     IGPLRDLFARRARRVNR"
     gene            3943..>5084
                     /locus_tag="VFQ35_22355"
     CDS             3943..>5084
                     /locus_tag="VFQ35_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933471.1"
                     /translation="MTVPRTGPTRAGTLPRPESEHTLSRRAFDEPRRRRLAAVATLPL
                     FFFLEGTAHAQSTPSAATPVAPSAATPEPAPAAGSCIERLPEGKARPKLTEKMASRGI
                     TGHAALLELVIEHGPGETVLPSGIGFQSSSDEYKALEAAHFFLPDPKGPAKPSVQRTQ
                     TGDKATTTVKLWFLPLPPKPGRNELTLPSLPIAISRASGERMTLCTSPHTLSLEEPTA
                     NEANPEPKGNPPARIQREEWTAAKQVTIAAAIALVLGALLVLLWQFWRKRPRALPPPP
                     PKRPPWETAFEALHDLRLSGLLSEQRYGEFYDRASDVVRRYLGDVYGYDGLESTTREA
                     LAALRRISMPMDVWVSIQEFMQDADLVKFARNDPTEAQCASALTRA"
BASE COUNT          964 a         1732 c         1629 g          759 t
ORIGIN      
        1 ttcggcggcg agctccggct cgaaagggcc gccatcgccg cggcatgggg cttatgtaag
       61 tcacgagccg cacgcgacgc ggcctgggca gaagcgcggc ggcggcagga aacaatcgtc
      121 cgaatcccac ccgggctggc cgaccgaacc cacgcaacaa gcgtgcagct tcagcgcgcg
      181 ttcatccgac ctcgctacaa atgtggacgc tcggagcccg aagtgtaact ctggggaaca
      241 agagcgagtg gcttctctag agggaatttc gcaaggcgcg acgctaggtc cttacgttgt
      301 ccaggaacgt cttcccggta acgagcgcgc atttttttcc gcgaagaccg caagcgggaa
      361 acaagtctgc ttgctggagc tactgggcga cgaggtggag tcgtatacgg ccgccaccgc
      421 ccacgacctc gatcatgctc acctcgcccc ggtgcacgag gtcttcacgc acgagggggt
      481 agctctcctc gtcttcgaca agatcgacgg catcttgctg acgaagcggt tggcggagat
      541 cggtaaaaag cccgagctgg aggcggtggg tgcggcgttg cgtgtttcgg acgcgctctc
      601 gaacctgcac gatgcgggcg gtgtgcacgg cttcattcac acggacagcg tgctcgtcga
      661 tccgccggag cgcctgggcc cagtgctcac gtttgcgccg atcccttccg acgagcgaac
      721 gtttcacagc cccgagcgag gcgaaaccgg aaagccgtcg gtcgcggacg atgcgtgggc
      781 cgccgcgggg ctcttgcatc agatgctcac gagcaagccg ccgcccaaat ccgggtactc
      841 gacggtcgaa gagttggagg aggccggcgt cgaaaatctc gcgctacgcg aagcgctgct
      901 gcacggcttg aacagcgatc cagagaagcg gagcaaagac gtccgtcctc tgaagcgaga
      961 gctggcacgt tggttcgtcg agcacgccgg tggtgaagcc gtgctgctcg ggccgcattc
     1021 caccatgccg cccccgctcc cagcgggcgc aaaggggctt gcccagggcg taccccaaac
     1081 tgcagtcgag gtcgggccga ggccgcgcgc gaaaaagcag ccgcgcttcg tcgtgctggc
     1141 gctcgcgggg acttcgctgg ggctcatcgc ggtgtgggcg tactcgatga tgcaccccaa
     1201 gccggtaccg ccagcgcccg tcgccgccgc cgccacgccg gctgcgtccg aaccgccgag
     1261 caccgccgca aaagcgatcg acctctccga ggtgccggtc acgggcgaag aacgcagcat
     1321 cgagctcggc aaaaccggct cgtgcgtcgc gagttacctg ccgaaaggcg cattttcgaa
     1381 ggagcccgac ctcgggagtc tgtgcagctc gaccgatccc aacaagggtg cacaggatct
     1441 gcgcgtcgcg ctcgtgaaca gcgcgccaaa gggcgccgtg accgacgcga tgaagctctg
     1501 gtcacggctc ggctggtacg acatggccgc gtacgccgtc gtgcgcggcg ggtgctgcga
     1561 agagcccaaa gacctcacgc tcgccgaacc cgccaagggc tgcgacgcaa tcgccgaggc
     1621 gctgcgcgat ctcagcaaag acgtcatcgg cggtaagccg tacgaagagt cgctcaagaa
     1681 gtacacgggc gcgattcatt gcgagctcaa cgcggggcac acgcaggtac cggggcgcac
     1741 gcagcgtccc cagcctggtg aagacaccgc gttcaaagac ttcgtcgcgg ccatccagca
     1801 ctgaaggttc gcgcgaccac gagacggctc gcgcaaccac cgttggccga accaagcgct
     1861 tcggcgaagg gcccagcggc agcgaccgcc gaccgaccaa tgggccgata gcgccgtgtt
     1921 acgctcgtcg ggaatacgcg gaaagcccgg attgtctctc gcgagccggt cgcgctatag
     1981 cggcgcctct ggcacgtcgg cgcgacgcta agccgcgccg aatccgagac ggtgacagca
     2041 atgcaagacg tccgagcgct caacgacctg gttgccaaag aaagcgcctt catcgacgac
     2101 ctgacctccg aaatcggcaa ggtcatcgtc gggcaaagct acatgatcga gcgagtgctg
     2161 gtcggcttgc tcgccggcgg ccacgtgctg ctcgaaggcg tccccggact cgcgaagacg
     2221 ctcaccgttc gcacgatttg cgacgccatc cacgcgaagt tctcgcgcat tcagttcacg
     2281 cccgacctcc tgccggcaga tctcatcggt acggtcatct acgaccagaa gacgggcgaa
     2341 ttcgcgagca agctcggacc catcttcgcg aacctcgtgc tcgccgacga aatcaaccgc
     2401 gcgccggcga aggtgcagag cgccctgctc gaggccatgc aggagcgcca ggtaaccatc
     2461 ggtgaccgct cctatcctct gcccgaccca ttcgtggtga tggcgacgca aaacccgatc
     2521 gagcaagaag ggacgtaccc gctacccgag gcgcaggtcg accgcttcat gctgatgatc
     2581 aaggtgaact atccgacgcg cgaagaagag cgcgccgtga tggatcgcat gaccgggccg
     2641 ctcaccgcct cggcgagccc acggacgagc ccggagcgcc tactcgaagc gcgcaaggtc
     2701 gtgcgcgacg tctacgtcga cgatcgcgtg aaggactaca tcgtcgacgt ggtgttcgcg
     2761 acgcgcgagc cttccgcgaa gggcatgaaa gagctcggac cgctcatcga gtacggcgcg
     2821 agcccgcgcg ccagcatcgc gctgaacctc gccgcgcgcg cccacgcctt cttgcgccac
     2881 cgcggctacg tcacgcccga agacgtgaaa gcgatcggcc ccgacgtact ccgccaccgc
     2941 atcgtgctct cgtacgaggc cgaggcggag gaggtcacgg ccgaggacat cgtgcgccgc
     3001 gtgttcgagg tcgtggaggt cccctgaacg gcgccgttcg aggggtcttc cgaggagcat
     3061 cagtgtgatc ccgaaggagc tcatcaaggc cctccgcaag atcgagatca ccaccaaccg
     3121 gctcgccacc gagcagttgt ctggcaacta tacgtccgtc ttccgcggac aaggtctcgc
     3181 cttccgcgaa gtgcgcgaat accagccggg cgacgacgtg cgcaccatcg actggaacgt
     3241 cagcgcgcgc atgaacaaca cctacgtcaa ggtgttcgtc gaagagcgcg agatgacggt
     3301 gatgctcgtc gtcgatctca gccagagcca gcagttcggc acccagcgcg gcagcaaggc
     3361 cgctctcgcc gcagaggtct cggcgctctg cgcattcagc gccatcaagc acaacgaccg
     3421 tgtcgggctc atcctctcca cggatcagat cgagaaaatc gtgccgcccc tcaagggaca
     3481 gaagcacgtg atgcgcgtcg tgcgcgaaat cctcggcgca aagcccgagc gccccggcac
     3541 cgacctcaaa atcgcgctcg aaacgctgta ccgcgtcgct cgtcggcgca gcgtcgcctt
     3601 cgtgctcagc gacttcttcg cgaccgacta cgagcgcgcc ctctccctcg ccgcggcgcg
     3661 ccacgacgtc atcccggtga tgctcgtcga cccgcgtgac gacgagctcc ccgacgtggg
     3721 cctcgccgcc ttcgaagatc tagagacggg cgagagcgtg ctcgtcgaca ctggcagcaa
     3781 gagcgtaaga gcgcactacg cgcgacgcat gcgcgaacag cgcgagaccc agatcaagct
     3841 cttccgaaag ctcggcttgg atcacgtcgt cgtgcgcacg gatcgcccct acatcggccc
     3901 actgcgcgac ctctttgcgc gccgcgcgcg gagggtcaac cgatgaccgt gccccgcacc
     3961 gggccaaccc gcgccggcac tcttccgcgg cccgagtcgg agcacaccct ttctcgtcga
     4021 gctttcgacg agccaaggcg acgaaggctc gccgccgtcg cgacacttcc cctcttcttt
     4081 tttttggagg gaacggcgca cgcgcagagc acgccgagtg ccgcgacgcc tgtcgctccg
     4141 tctgccgcga cgccagagcc agcgcccgcg gcgggaagct gtatcgagcg ccttcccgaa
     4201 ggcaaggcgc gcccgaagct caccgaaaag atggcgagcc gaggcatcac cgggcacgcc
     4261 gcgctgctcg agctcgtcat cgagcacggg ccgggcgaga cggtgctccc gagcggcatt
     4321 gggtttcaat cgagcagcga cgaatacaaa gcgctcgaag ccgcgcattt cttcctgccc
     4381 gatcccaaag gtcccgcgaa gccgagcgtg cagcggacgc aaacgggtga caaggcgacc
     4441 acgaccgtca agctgtggtt cttgccgctg ccgccgaagc ccgggcgcaa cgagctcacg
     4501 cttccgtcgc tgccaatcgc gatttcgcgc gccagcggcg agcgcatgac gctctgcacg
     4561 agcccgcaca cgctgagcct cgaggagccg actgccaacg aggcaaaccc ggaaccaaag
     4621 ggcaatccgc ccgcgcgcat tcagcgcgaa gagtggaccg ccgcgaagca ggtcaccatc
     4681 gcggccgcga ttgcgctcgt gttgggcgcg ctgctggtgt tgctgtggca gttctggcga
     4741 aagcgaccgc gcgcgttgcc gccgccgccg ccgaagcggc cgccctggga gaccgcgttc
     4801 gaagccctgc acgatttgcg cctttcgggg ttgctcagtg agcagcgcta cggcgaattc
     4861 tacgatcgcg cttccgacgt cgtccgccgc taccttggcg acgtctatgg ctacgacggg
     4921 ctcgaaagca cgacccgcga ggcgctcgcg gcgctccgcc gcatctcgat gccgatggat
     4981 gtgtgggtct cgatccaaga gttcatgcag gacgcggatc tggtgaagtt cgcgcgcaac
     5041 gatccgacgg aagcgcagtg cgcgagcgcg ctcacccgcg cgga
//
LOCUS       DASTKS010001060         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1955124, whole genome shotgun sequence.
ACCESSION   DASTKS010001060 DASTKS010000000
VERSION     DASTKS010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955124"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1080)
                     /locus_tag="VFQ35_22360"
     CDS             complement(97..1080)
                     /locus_tag="VFQ35_22360"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9933472.1"
                     /translation="MASCASNRLALMKLYASPLSCSMATKIVLEELGRACEVIYVDAT
                     TRLAPDGFAVPLGQVPALRLEDGRVLTENAAILRFVAEGTELVPTESWQRTELTRWLA
                     FIGTELHKVVFIPLLDPKADEGVHAYALDKAPSRLRLVDEELSRRNHLLESYSVADIY
                     LATVLNWAQATPLRLDEYPGLLSFMARMKARPTVARILAEEHARYRAERAHSSARHLG
                     TRAVLDRFNQVFQRREPAALDSLVADGCVLENTDGKRLEGKAAAVGLWTSIATDASLS
                     FDLKDVRTSGDVGIITWVLLRDGRPVGDGINVMRVRDGRVWEARGYMRPSS"
     gene            1305..1727
                     /locus_tag="VFQ35_22365"
     CDS             1305..1727
                     /locus_tag="VFQ35_22365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933473.1"
                     /translation="MLNDILYVEDNLDHAELVLRQLERRGLRERVVHVEDGERAVAYL
                     DRVASGDSLPPRLVLLDLRLPRVDGMDVLSHVKGSAVLRDIPVVIFTTSTTPHDLKEA
                     YSRYANSYLVKPDDLTELNHVLDELSHYWLEMNRRSAG"
     gene            1724..>2951
                     /locus_tag="VFQ35_22370"
     CDS             1724..>2951
                     /locus_tag="VFQ35_22370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933474.1"
                     /translation="MTDARVILLVEDDSAHADLALRALERCDGLTVLVVDCVEQAREV
                     LARQHVDLVLADLRLPDGSGLDLLQQEIPVVVQTSQGDESRAVAAMKGGALDYCVKSP
                     EMFRDLPIIVERALNAGRNLFERRRAERSLRESDERFRQLAANIPEVFWLFDLASRQV
                     EYASPAWESVYGPVPQTWEGRLQYAHADDAAPLWALGPSDGDLFRVVEGTSLRWVEER
                     RFWINDAQAKTSRLACLAVDVTRRRELEASLSHTQKMHAIGQLAGGVAHDFNNMLAAI
                     LSAAEELSESKADPELCELIISASMRASELTRNLLSFSRRGKMELRVLDMHDVVRLAV
                     TLLSRSINPNVRLITSLDATRAEVLGDPSQLESAILNLGLNARDALPPRGGDICFSSR
                     ELVFDEASSERKALNLE"
BASE COUNT          528 a          952 c          944 g          527 t
ORIGIN      
        1 gaaggctccg cgcgacaatt cgcctccgtg ctgcagactt cggcgcccca cctcccctca
       61 cggtagggcg cgcgagccgc cgcctgcctt tggtaactac gagctcggcc gcatgtagcc
      121 ccgagcctcc caaacgcgac cgtctcgaac acgcatgacg ttgatgccat cgcccacggg
      181 gcgcccatcg cggagcagca cccacgtgat gatgccgacg tctccgctcg tgcgtacgtc
      241 cttcaggtcg aacgaaagcg aagcatcggt cgcgatcgag gtccataacc cgaccgccgc
      301 tgcctttccc tcgaggcgct ttccgtccgt gttctccagc acgcagccgt cggcgacgag
      361 ggaatcgagc gcagcgggct cgcgacgctg aaacacctga ttgaagcggt cgagtaccgc
      421 tcgcgtgccg aggtgccgcg ccgacgaatg cgcgcgctcc gctcggtagc gcgcgtgctc
      481 ttcggcgaga atccgcgcca cggtcggccg cgccttcatg cgcgccatga agctcaggag
      541 ccccggatac tcgtccagcc ggagcggcgt cgcttgggcc cagttgagca cggttgccag
      601 gtagatgtcg gcgaccgagt agctctcgag caggtggttt cgtcgcgaga gttcctcgtc
      661 gacgagtcgc agacgactcg gcgccttgtc gagcgcatag gcgtgaaccc cttcatcggc
      721 cttgggatcg aggagcggga tgaagaccac cttgtgaagc tccgtcccga tgaacgcgag
      781 ccaacgcgtg agctccgtgc gttgccagga ctccgtcggg acgagctcgg ttccctcggc
      841 gacgaaccgc aaaatggccg cattttcggt gagcacccgg ccgtcttcga gcctcagcgc
      901 tggcacttgc ccgagcggca ccgcgaagcc gtcgggagcg agacgagtcg tcgcatcgac
      961 gtaaatcacc tcgcacgcgc gaccaagctc ctcgaggacg atcttggtgg ccatggaaca
     1021 ggagagcgga gaagcataca gtttcatgag cgccagtctg ttgctcgcgc acgatgccat
     1081 ccaacgatat tatcggatac gagctatgcg cgcttcgcat cgctctgcct tagtcacagg
     1141 caaactgttc gagcgatgcg tttcgtcatg gcgattggcg tcaaggcgcg gccgagcgcg
     1201 ccgtggccgg gcccattcaa ccgattcgcg aaggcatccg cggcaatgca cccctcacgc
     1261 tttcctggac ccatggcgga tgataacctg cgcgctggtg cagtatgctc aatgacatcc
     1321 tttacgtgga ggacaacctc gaccatgccg aactcgtgtt gcggcagctc gagcgaagag
     1381 ggctccgcga gcgcgtcgtt cacgtggagg atggagagcg cgctgttgcc tacctcgatc
     1441 gggtcgcgag cggcgattct ctgccgcccc gtttggtact gctcgacctg cggttaccgc
     1501 gcgtcgacgg gatggacgtc ttgtcccacg tcaaaggatc ggcggtgctg cgtgacattc
     1561 cggtcgtcat cttcacgacg agcacgacgc cgcacgattt gaaagaggcc tattcgcgct
     1621 acgcgaacag ctatctcgtc aagccggacg acttgacgga gttaaatcac gtgttggacg
     1681 aactgtccca ttactggctc gagatgaatc gtcggagcgc aggttgaccg acgcgcgcgt
     1741 catcctcctc gtcgaagatg acagcgctca cgccgacctc gcgttgcgcg cattggagcg
     1801 ctgtgacggc ctcaccgtat tggtagtcga ctgcgtcgag caagcacgag aggtgctcgc
     1861 gcgacagcac gtggacctgg tgctcgccga tctgcgcttg cctgatggct cggggctcga
     1921 cttgctccaa caagagatcc cggtcgtggt gcagacgagc caaggcgacg agtcacgcgc
     1981 cgtggccgcg atgaagggcg gggcgctcga ctactgcgtg aaatcgcccg agatgtttcg
     2041 cgatcttccg atcatcgtcg agcgggcgct caacgcgggg cggaacctgt tcgagcgacg
     2101 acgcgcggag cggtccttgc gagagagcga cgagcgcttc cgtcagctcg ccgcgaacat
     2161 cccagaggtg ttctggttgt tcgatctcgc gtcccgccag gtcgagtatg cgagcccagc
     2221 gtgggagtcc gtctacggac cggtgcccca gacctgggag gggcgactgc agtacgctca
     2281 cgcggacgac gcggcgccgc tctgggcgct cgggccctcg gatggcgacc tgtttcgcgt
     2341 ggtggaggga acgtcgctgc gctgggtgga agagcgacgc ttctggatca acgatgctca
     2401 ggcgaagacg tctcggctgg cgtgcttggc ggtggatgtc acgcgacgcc gcgaactcga
     2461 agcgtcgctg agtcacaccc aaaagatgca cgcgataggt cagctcgccg gtggcgtcgc
     2521 tcacgacttc aacaacatgc tcgcggcgat cctctcggcg gccgaagagc tgtccgaaag
     2581 caaagccgac cccgagctct gcgagctcat catttccgca tcaatgcggg ccagcgaact
     2641 cacgcgcaac ctgctctcct tctcgcgtcg cggcaagatg gagctgcgcg tattggacat
     2701 gcacgacgtg gtccgcctag cggttaccct cttgagtcgc agcatcaacc cgaacgtgag
     2761 gctcatcact tccctcgacg cgacacgcgc ggaggtgctc ggcgacccat cgcagctcga
     2821 gagcgccatc ttgaacctgg gactcaacgc gcgcgacgcc ctgcctccgc gcggaggcga
     2881 tatctgcttc agctctcgag agctcgtgtt cgacgaagcg tcgagcgagc ggaaagcgct
     2941 gaatctggag c
//
LOCUS       DASTKS010001061         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_167630, whole genome shotgun sequence.
ACCESSION   DASTKS010001061 DASTKS010000000
VERSION     DASTKS010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167630"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFQ35_22375"
     CDS             complement(<1..323)
                     /locus_tag="VFQ35_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9933475.1"
                     /translation="MGSSAARVERGRLHGDVPVRELEIAGAAPRVLALHGFGGTPLEV
                     EAVLDSARKLGLGGLAPLLPGHGTHAKSLASMRFPDWSDAAERALDRVGGPVVVAGFS
                     LGSLLA"
     gene            complement(298..1746)
                     /locus_tag="VFQ35_22380"
     CDS             complement(298..1746)
                     /locus_tag="VFQ35_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9933476.1"
                     /translation="MRDAREVLPLAPVAPTALVVDDEKNIRRTLEMVLSGEGYRVLEA
                     ESAEQALELIRNPRQPVDVAIFDLKLPGISGLEALEKLRADDATRELPVIVISGHATV
                     HDAVSAIKLGASDFFEKPLNRERVLVSVRNSVRAARMSQTLEQMRGELEARYEMIGTS
                     PPIQKLFQDIDKVAPTKASVLITGESGTGKELVSRAIHRLSPRKDAPFVKVNCAAIPR
                     ELIESELFGHERGSFTGAQGRKRGFFEQAHGGTLFLDEIGDMDLTAQAKVLRALQNGE
                     IVRVGSEHVIHVDVRVLAATNKDLAKSVAAGTFREDLMFRLDVFPVRVPALRERPGDI
                     PLLADAFVAAFCKENGLRPKRIDPSLYEALSRRKWPGNVRELKNVVERAAILSSDVIT
                     VADLPEDPHSSPFEDESEDETALPNAAAQPDATRGRLTLREHRDAAERAYILDTLKEL
                     SWNISRASVVLGVERTNLHKKIRAYGIKRGES"
     gene            complement(1969..2871)
                     /gene="bamD"
                     /locus_tag="VFQ35_22385"
     CDS             complement(1969..2871)
                     /gene="bamD"
                     /locus_tag="VFQ35_22385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9933477.1"
                     /translation="MHSKADPIPALPGAASARPLRFSRYLAALALAASLGLPACAADD
                     KRPMTALDYSENAKRDYEKAMRALEEKNWEQVEPLFNEVRKNYSYSRYARLAELRIAD
                     ANYRQDKLPEAISGYRSFLHDYPNDPEVPYARFQIARAEYDSVSLSPFLPPLEERDLT
                     SVNDALATIRGYLADYPNTEHTEELRYMLEVVLGLLARHELYVARYYLSRDRFDAAAA
                     RVDHAVSAFPRSGLEAEGLLLLAEIRMKQKRPDDARKLCARLLKEHPESPFSVAAKNY
                     LARLGPDETVGVLRQSATPATATP"
BASE COUNT          457 a          952 c         1065 g          531 t
ORIGIN      
        1 gcgagcagcg aaccgagcga gaaaccggcg acgaccacgg gtccgcccac ccgatccagg
       61 gcgcgctcgg ccgcgtccga ccagtccgga aagcgcatgc tggcgaggct ctttgcgtgg
      121 gtgccgtggc cggggagcag cggggcgagc cctccgagcc ccaacttgcg cgccgaatcg
      181 agcaccgctt cgacctcgag cggcgtgccg ccgaagccgt ggagcgccag gacgcgcggc
      241 gccgcccccg caatttctag ctcgcggacg ggaacgtcgc cgtgtagtcg gccacgctca
      301 actctcgccg cgcttgatcc catacgctcg aatcttcttg tgcaggttgg ttcgctccac
      361 cccgagcacg accgacgctc gtgagatgtt ccacgacagc tctttcagcg tgtcgaggat
      421 gtaggctcgc tctgccgcgt cgcggtgctc gcgcaacgtg agccgtccgc gcgtcgcatc
      481 tggctgggcc gccgcgttgg gcagcgcggt ctcgtcttcg ctctcgtcct cgaacgggct
      541 cgagtgcgga tcttccggca agtcggcgac ggtgatgacg tcgctcgaca agatcgccgc
      601 ccgctcgacc acgttcttca gctcacgcac gttgcccggc cacttgcgcc gcgacagcgc
      661 ttcgtaaagc gacggatcga tgcgcttcgg acgcagcccg ttctctttgc aaaacgccgc
      721 gacgaaggca tcggccagca agggaatgtc gcccgggcgc tcgcgcagcg cgggcacccg
      781 aaccggaaag acgtcgaggc gaaacatcaa gtcttcgcgg aatgtccctg ccgcgaccga
      841 cttggcgaga tctttgttgg tagcggcgag cacccgcacg tcgacgtgga tcacgtgctc
      901 ggagccgacc cgcacgatct cgccgttttg cagggcccgc agcaccttcg cctgcgccgt
      961 gaggtccatg tcgccgatct cgtcgaggaa cagcgtgccg ccgtgcgctt gctcgaagaa
     1021 gccgcgcttg cgaccttgcg ctcccgtgaa ggaaccgcgt tcgtggccga agagctcgct
     1081 ctcgatgagc tcgcgcggga tcgccgcgca gttcaccttc acgaacggcg cgtccttgcg
     1141 cgggctcagc cgatggattg cgcggctcac gagctccttg ccggtgccgc tttcgccggt
     1201 gatgagcacg ctcgccttcg tgggcgcgac cttgtcgatg tcttggaaga gcttttgaat
     1261 cggcgggctc gtgccgatca tctcgtagcg agcctcgagc tcgccgcgca tttgctcgag
     1321 ggtctggctc atgcgcgccg cgcgcaccga gttccgaacg ctgacgagca cgcgctcgcg
     1381 gttgagcggc ttctcgaaga agtcggacgc gccgagcttg atcgcgctca ccgcgtcgtg
     1441 gacggtggcg tggccgctga ttacgatgac cggtagctcg cgcgtggcgt cgtcggcgcg
     1501 cagcttttcg agcgcttcga gcccgctgat gcccggcaac ttcaaatcga aaatggcgac
     1561 gtcgaccggc tgacgcggat tgcggatgag ctcgagcgct tgctcggcgc tctccgcctc
     1621 gaggacccga tacccctcgc cggaaagaac catctccaag gtgcgtcgga tgttcttctc
     1681 gtcgtcgacc accagcgccg tgggggccac gggggccaga ggcaagactt cgcgcgcgtc
     1741 acgcattcac ccgagggtac acgatcagcg ggcggggggg agcggtccgg gggggaggcg
     1801 tttcgcgctc ggcgcttcgc gcctgcgtgg cgacaggggg cgtctcgcgg ggtctctttc
     1861 gcgaccggaa agcttggcgg caaagggtta tcgcggcctt cgctgcggaa gggttcgttc
     1921 ccgatgcttc gtttgtgtgg tgaaaggggg cccggctcgg cgcgacggtc agggggtcgc
     1981 ggtagcgggc gtcgccgact gacggagcac tccgaccgtt tcgtcggggc cgaggcgggc
     2041 caggtagttc ttggccgcga cgctgaatgg gctttcgggg tgctctttga ggaggcgtgc
     2101 gcagagcttg cgcgcgtcgt cgggacgctt ctgcttcatg cgaatctcgg cgaggagcag
     2161 gagcccctcg gcctcgagcc cggagcgagg gaaggcggag acggcgtggt cgacgcgcgc
     2221 ggcagcggcg tcgaagcggt cccgcgagag gtagtagcga gccacgtaga gctcgtggcg
     2281 cgcgaggagg ccgagcacga cttcgagcat gtaccggagc tcttcggtgt gctccgtgtt
     2341 cgggtagtcg gcgaggtagc cgcgtatggt cgcgagcgcg tcgttgaccg aggtgaggtc
     2401 gcgctcctcg agcggcggca agaagggaga aaggctgacc gagtcgtact cggcccgcgc
     2461 gatctggaag cgggcgtacg gcacttccgg atcgttcggg tagtcgtgca agaacgagcg
     2521 atagccggag atcgcctccg gaagcttgtc ttggcgatag ttcgcatcgg cgatgcgaag
     2581 ctcagccagc cgagcgtagc ggctgtagct gtagttcttg cggacctcgt tgaagagcgg
     2641 ctcgacctgc tcccagttct tctcctcgag cgcgcgcatc gctttttcgt agtcgcgctt
     2701 cgcgttctcg ctgtagtcga gcgcggtcat gggccgcttg tcgtcggccg cgcacgcggg
     2761 caagcccagg cttgcagcga gggcgagcgc cgcgagatag cgggaaaaac gcagcggccg
     2821 cgctgatgcg gcaccgggta gggcagggat tggatcggct ttcgagtgca acttcggacg
     2881 gttccggcga acgaagggac tttcccttag cgggtcgagg ctcggaccgc aagtccgacg
     2941 cgccaagaag agaaggctcg cttcaggatt ggcgtccagt cgcggccgct tttaagatgg
     3001 gccgc
//
LOCUS       DASTKS010001062         5334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_389775, whole genome shotgun sequence.
ACCESSION   DASTKS010001062 DASTKS010000000
VERSION     DASTKS010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389775"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..590
                     /locus_tag="VFQ35_22390"
     CDS             198..590
                     /locus_tag="VFQ35_22390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933478.1"
                     /translation="MIRSAQTLAGRHIVVAEDVEAVRRTIERGLSRAGARVTCKEDGR
                     AALELLSSGVEVDLVLTDVVMPRMGGVELARELGKIRPELPIAFMTGYADVESIQQLE
                     SREPHLPVLNKPFTIDDLVTAVHRALER"
     gene            942..2207
                     /locus_tag="VFQ35_22395"
     CDS             942..2207
                     /locus_tag="VFQ35_22395"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET9933479.1"
                     /translation="MPTSGPSIRPASLSPLCVAFALALGACSGEDPNVTQQQPAPAAT
                     ADAVDVPIRGLDVEWISEFNDGDLAFGTPLREADGLGPLYTRASCAACHDTGARGPGL
                     VQKMVLVEDDGVTPRPDQSALAFGFTVHPLTAGGGHSPILPPKDPGVKVTDRLGPPVI
                     GRGYMEAIADSEIERVAQEQAAAGGDVHGRVNYVSYASEPNSELRFHQHAPGDRVIGR
                     FGLKARVATLDDFTADAFQGDMGITSPLRPTEFPNPDGLTDDMKPGIDVGFESVNRRA
                     NYVRLLAIPARRTQATAEALFASTGCASCHVPSLRTRADYPVAALADVDAPIFSDLLL
                     HNMGAELADGLPRNSVDGDAGSFEWRTAPLIGLRFNRSYLHDGRAKTIEQAILLHRGE
                     GSEANASIDAFETLTASDRQNLVSFVSAL"
     gene            2213..3274
                     /locus_tag="VFQ35_22400"
     CDS             2213..3274
                     /locus_tag="VFQ35_22400"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HET9933480.1"
                     /translation="MNFPMLSRIALLSCALWLTACSSDDDGPKTDADYSRAVALQMHD
                     VLGKDVKALHTAAVELKAAAPNVDRGWTDADTAAITATTNAWIRARSAYERSEGAIAP
                     IFPDIDAAIDARYDDFLETYSPDGDPNLFDGERVTGMHAIERILFAKTTPQAVIDVEA
                     TLPGYVAARWPESAEESKAFKNELCAQLVTDTAALEAEWASNPIDLQSAFGGLIALMN
                     EQREKVRKAASAEEESRYAQRTLADIRDNLAGTRAAYALFQPWVRSKQGGADVDASIQ
                     GAFKKLEDAYASLPGDAFPPLPADFSAEAPTEANLATDFGRLFAEVESAANPNREGSV
                     VHGMNQAAVLMNFPEFVDQ"
     gene            3271..3804
                     /locus_tag="VFQ35_22405"
     CDS             3271..3804
                     /locus_tag="VFQ35_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933481.1"
                     /translation="MNTKLRYAFALCALLAACSGSSASDDSPSSTSSFVAFANDFKGY
                     HSWQSFDVSAEAKEKGIHDGTVIEYVNQLPASGSTEYPLGTLVVKEAIGGAMGNALFA
                     MARRGGNYNSTGVRGWEWFELENLATEDDSVKIIWRGFGPPIGEEYGGDPNSTCNTCH
                     KKCVDAVCSTPLALENF"
     gene            3819..4628
                     /locus_tag="VFQ35_22410"
     CDS             3819..4628
                     /locus_tag="VFQ35_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933482.1"
                     /translation="MRPFVPLLASALALVAVRAEATPRPLPFTYQHEQLDAGEAELEQ
                     YADFTPVRARAAESADLRYYGLTQFQTEFEYGLTSRLELGLYLTYVPTTTNGFAEVPR
                     ATEGNGLKQRLRWQLAPTGEWPIDVALYGEVVENEREVELEAKVILQRRFDRLRLIAN
                     IVGEREFYYDGTHDLVFAPSAGVTFEASPRIQPGFEWWMRSEYPEENAPSPRPFSLGP
                     HHYAGPTLMAFLGRTFVTTGAYVRLSDTSHTMALGEGFGRVWFRMLVGVGL"
     gene            4636..5073
                     /locus_tag="VFQ35_22415"
     CDS             4636..5073
                     /locus_tag="VFQ35_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933483.1"
                     /translation="MRRSVLVGLTLLAACNSPQKAREPGGSPSAESAPLARSDPADPA
                     AECKARCNGEFAAHGLNGTPHCLCRTRDFGKSCESSGECEGQCIADPALTRVTDAGPP
                     KRGFYVGRCSEFITSFGCSPILPKAKPGGPVEVGEPPATVCMD"
BASE COUNT          959 a         1797 c         1701 g          877 t
ORIGIN      
        1 atcgagcctt ctatgacgct acttctcgag ggttgaacgc agcgctagta aaaagaaatt
       61 cctcgcccat tgctggccgc atcgctattc ttggactgga tgcagcccca atcgcatcac
      121 gctgaattgt cagtcgattc cgtggctgtt gccgttggga ggctcggggc aagacccgcg
      181 cgcgaaagcc tggcgccgtg atccgcagcg cgcagacgtt ggccggacgg cacatcgtcg
      241 tcgccgaaga cgtcgaggcg gtgcgtcgca ccatcgaacg cgggttgtcg cgcgccggag
      301 cgcgggtcac ctgcaaggaa gacgggcgcg ccgcgctcga gctgttgagc tccggggtcg
      361 aggtcgatct ggtgctcacc gacgtcgtga tgccacggat gggcggagtc gaactagcgc
      421 gtgaactcgg gaaaattcgg ccagagctgc ccatcgcttt catgaccggg tatgccgacg
      481 tcgagtcaat ccagcagctc gagtcgaggg aacctcacct gcccgtcctg aacaagccgt
      541 tcaccatcga tgatctggtg accgctgttc atcgagccct cgaacgctga gggcccgcga
      601 atgaacaagt cagtcgcgag cgggccgaga ccgcagcgag gcgggcgcgt gaggagagag
      661 caccgcaggc ctgctgaagc aggtcgagga gcgcaacgac gagcccgccc gccgcagcaa
      721 gaggatcggc ccgcgcagta tgacttgtcc gttcgcgggc tctaaggccc gcaggtgcgg
      781 ccgggcaacg cgcgcaacct actggatccc gaaggaattt cagccgcgac aagggcgcgc
      841 ccggcgcggg cggacgagcc aggtgcgagc gctgaccggc gtcatccggc tcccgagctg
      901 agtttgacaa tgactttcac tttcaatagg gtgtgcgagt catgcccacc tcgggaccgt
      961 ccattcgccc cgcatcgctc tcgccgctct gcgtggcctt cgcgttggca ctcggcgcgt
     1021 gcagcggaga agaccccaac gtcactcaac agcagcccgc acccgcagcg accgccgatg
     1081 cggtggacgt gccgatccgt gggctcgacg tggagtggat ttccgaattc aacgacggcg
     1141 acctcgcctt cggcactccg cttcgcgagg cggacggact cgggccgctc tacacccgcg
     1201 catcgtgcgc tgcctgccac gacaccggcg cgcgcggccc gggcctggtt cagaagatgg
     1261 tgctggtcga ggacgacgga gtgacgcccc gccctgacca atcggcgctc gcgttcggct
     1321 tcaccgtgca cccgctcacc gccggcggcg gccactcacc catccttccc ccgaaggatc
     1381 cgggcgtgaa agtgacggat cggttgggcc cgcccgtcat cggtcgcggc tacatggaag
     1441 caatcgccga cagcgagatc gagcgagtcg cgcaagaaca agcggccgcg ggcggagacg
     1501 tccacggtcg agtcaattac gtgagctacg cgtccgagcc caacagcgag ctgcgctttc
     1561 atcagcacgc gccgggtgat cgggtaattg gtcgcttcgg gctgaaggcg cgggtcgcga
     1621 cgctcgacga cttcacggcg gatgcgttcc agggcgacat gggcatcacg agcccgctgc
     1681 gacccaccga gttcccgaac ccggacgggc tcaccgacga catgaagcca ggcatcgacg
     1741 tcgggttcga gagcgtgaac cgacgcgcga actacgttcg cctcttggcg atcccagcgc
     1801 gacgcacgca agcgacggcc gaggcgctct tcgcgtccac cggttgcgcg agctgtcacg
     1861 tgccctcgct ccgcactcgc gccgactatc cggtcgcggc gctcgccgac gtcgacgcgc
     1921 ccatcttctc cgacttgctt ttgcacaaca tgggcgccga gctggccgac gggttgccca
     1981 ggaactcggt cgacggagac gcgggctcgt tcgagtggcg caccgctccg ctcatcgggt
     2041 tgcgcttcaa ccgcagctac ttgcacgacg gccgcgcgaa gacgatcgag caggccattt
     2101 tgctccaccg cggcgagggc tcggaggcca atgcctcgat cgatgcgttc gaaaccttga
     2161 ccgcgagcga ccgacaaaac ctggtctcgt tcgtctctgc cttgtgaaaa acatgaactt
     2221 tcccatgctt tcgcgtatcg ccctgctttc ctgtgcgctc tggctcaccg cgtgctcgag
     2281 cgacgacgac gggcccaaaa ccgacgcaga ttactcgcga gcagtggcgc tccaaatgca
     2341 cgacgtactc ggcaaggacg tgaaggcgct gcacacggcc gcggtagagc tcaaggccgc
     2401 ggcgcccaac gtcgatcgcg gctggactga cgcagatacg gctgccatca cggccacgac
     2461 gaacgcctgg atccgcgccc gctcggcgta cgagcgcagc gaaggcgcga tcgcgccgat
     2521 cttccccgac atcgacgcgg ccatcgacgc gcgctacgac gacttcctcg agacgtattc
     2581 gccggacggc gaccccaatc tgttcgacgg cgaacgcgtg accggcatgc acgccatcga
     2641 acgcattctc ttcgccaaga cgacaccgca ggcggtcatc gacgtcgagg cgacgctgcc
     2701 aggttacgtc gccgcgcgct ggcccgagag cgccgaagag agcaaggctt tcaagaacga
     2761 gctctgcgcg cagctcgtga ccgacacggc tgcgctcgag gccgagtggg cctcgaaccc
     2821 gatcgacctg cagagcgcgt tcggtgggct catcgcgctc atgaacgaac agcgagagaa
     2881 ggtccgcaag gcggccagcg cagaggaaga gtcgcgctac gcgcaacgca cccttgccga
     2941 catccgcgat aacctcgccg gcacacgagc cgcctacgcg ctgttccaac cgtgggttcg
     3001 tagtaagcaa ggcggagcgg acgtggacgc gagcatccaa ggcgccttca agaaactcga
     3061 ggacgcctac gcgagcttgc ccggcgacgc ctttcctccc ctgcccgccg acttcagcgc
     3121 agaggcgccc acggaggcga acctagccac cgattttggt cgactgttcg ccgaagtcga
     3181 gagcgcggcg aaccccaatc gcgaaggctc cgtggttcac ggcatgaacc aggccgcagt
     3241 gttgatgaac tttccggagt tcgtagacca gtgaacacca agctccgtta cgcattcgct
     3301 ctctgcgcgc tgctcgcggc gtgcagcggt tcctcggcca gcgacgattc gccttcgtct
     3361 acgagctcgt tcgtggcgtt cgccaacgac ttcaagggct accactcgtg gcaatccttc
     3421 gacgtgtcgg cggaggcgaa ggagaagggc atccacgacg gcaccgtgat cgaatacgtg
     3481 aatcagctgc cagcgagcgg ctcgaccgag tatccgctcg gcaccctggt cgtgaaagag
     3541 gccatcggtg gcgcaatggg caatgccctg ttcgcgatgg cacggcgcgg cgggaactac
     3601 aactcgaccg gtgtgcgcgg ctgggagtgg ttcgagctcg aaaatctcgc gacggaggac
     3661 gactccgtga agatcatctg gcgcggcttc gggcctccca tcggcgagga atacggcgga
     3721 gatccgaaca gcacctgcaa cacttgtcac aagaagtgcg tcgacgccgt gtgctccacg
     3781 ccgctcgcac tcgagaactt ctaacttaga gctcattcat gcgtccgttc gttcctctcc
     3841 tcgcgagtgc gctcgcgctc gtcgcagtgc gggccgaagc gaccccgcgc cccttgccct
     3901 tcacgtacca gcacgagcaa ctcgacgcgg gcgaagccga gctcgagcag tacgccgact
     3961 tcacgccagt tcgcgcgcgc gccgccgaga gcgcggacct ccgctactac ggcctgacgc
     4021 aattccagac cgagttcgaa tatggcctta cgtcacgcct cgagctcggc ctgtatctca
     4081 cgtacgttcc gacgacgacc aacggcttcg ccgaggtgcc acgcgcaacc gagggcaacg
     4141 ggctcaagca gcggcttcgc tggcagctcg cgccgacggg ggagtggccc atcgacgtcg
     4201 cgctctacgg tgaggtcgtc gagaacgagc gtgaggtgga gctcgaggcg aaggtcatct
     4261 tgcagcgccg tttcgaccgc ctgcggctca tcgcgaacat cgtgggcgag cgagagttct
     4321 actacgatgg cacgcacgac ctggtgttcg ccccctccgc gggcgtgacc ttcgaagcat
     4381 caccgcgcat tcagcctggc ttcgaatggt ggatgcgcag cgagtacccc gaggaaaacg
     4441 cgccgtctcc gcgcccgttc agcctcggac cccaccacta cgccggaccc acgctcatgg
     4501 cctttttggg tcgcacgttc gtcacgaccg gcgcctacgt tcgcctgagc gacacctcgc
     4561 acaccatggc tctcggcgaa ggctttggtc gagtgtggtt tcgcatgctc gtgggcgtag
     4621 ggctatgatg agggcatgcg ccgctccgtc ctcgtcgggc tcacgctgct agccgcctgc
     4681 aactctcccc agaaagctcg cgagcccggc ggctccccaa gcgccgagtc ggctcccctc
     4741 gcgcgaagcg acccggccga tcccgcagcg gagtgcaagg cgcgctgcaa cggtgagttc
     4801 gccgctcatg gattgaatgg gacgccacac tgtctctgcc gtacacgcga cttcggcaag
     4861 agctgcgagt cgagcggcga gtgcgaaggg cagtgtatcg ccgacccggc tctcacgcgc
     4921 gtaaccgacg caggaccgcc gaagcgaggg ttttacgtcg gacgttgctc cgagtttatc
     4981 acctcgtttg gctgcagccc gattttgccc aaggcgaagc ccggtgggcc cgtcgaggtc
     5041 ggcgagcctc ccgccaccgt ctgcatggat tgaccaaaag ccgggccttg agacgatgtc
     5101 gcggcggcgc tgacgcgaaa ttttggcgcg ccagggcgag acccagcccg ggcagaggta
     5161 tacgctcccg ggcgtgtaca agagtccggc gcaaatcttg ggcgctctcg cggccgtcct
     5221 cgtgatgggc tggttgtttt gggtgaagct actgccaaag agctgccccg gcggcgtatt
     5281 cgtcgaattc catccacccc tttccgagcc cggaccgttc cacttccgcc tgcg
//
LOCUS       DASTKS010001063         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1060689, whole genome shotgun sequence.
ACCESSION   DASTKS010001063 DASTKS010000000
VERSION     DASTKS010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060689"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFQ35_22420"
     CDS             <1..503
                     /locus_tag="VFQ35_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933484.1"
                     /translation="AAQDVSVSKHASIRQQTLVLLNPPLDPFAAYFSIYREARGEPRP
                     KHLYWLATGVDSIVVTGVDEHTLRLRPTRGFLASSSQRMLRSAASGFERDQEVRLEDC
                     SIRVEELTLDRRPQAILVRFRSNLDDRALAFMTWQGPGYVAFVPPAPGQSVVIPRVDL
                     WKALFG"
     gene            complement(492..1496)
                     /locus_tag="VFQ35_22425"
     CDS             complement(492..1496)
                     /locus_tag="VFQ35_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933485.1"
                     /translation="MANLAPRPVRGRTDLFAEWFSALEAEGESMRFRLGRCDDQRSHP
                     TWHFLPHQDYDGIGGLAVVLRQLEGKSLDMPRLAEPYPSRARRWLAAARFALRRSHEA
                     IPFRDFDDAWTGDPRQPTAPSAYAWALLSESDTHELRQAARSRGVSLNAWMLWSLTRA
                     MLPHLQRDRGSVAWIVPVNMRGLRPELRDTANQAATFDVAFPAGANVAQVDEALKRER
                     EALAHFGVWQLLQLLEHLDPESIRRLAQRESRIKKHGSFSNLGSLRVSGSEFSPQSRE
                     FWMAFNPVIKSRPVGAACLTYEGRLALTLQLHPALSRDESTAHAWLLAWLQALFGSAE
                     "
     gene            1594..>2331
                     /locus_tag="VFQ35_22430"
     CDS             1594..>2331
                     /locus_tag="VFQ35_22430"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET9933486.1"
                     /translation="MTALPAFDADQLFDVQAPDETPLDWPGPGPIDRAVHDLPHASST
                     LEWWYLNCHLVTREGREVSLFAAFFRERAGVDPETGAAIYAHSVAWALSEPDRARYHP
                     KVAVDPRAPALGLAKLDAARHVSSGRIDRALREVLERGRVPGPTRLLDSPAVVSQSLL
                     HLDYGRDRLVKIDADEYELSLYDELAEIGCSLRFVPRKNPVRYGSDGIVHGVQDERMF
                     YYFIPRCDVRGQLTIDGSSVGVDQGSGW"
BASE COUNT          398 a          774 c          744 g          415 t
ORIGIN      
        1 ttgcagcgca ggacgtcagc gtgtccaaac acgcgagcat tcggcagcag acgctggttc
       61 tcctgaaccc tccgctagat ccattcgccg cctatttctc catctaccgc gaagcgcgcg
      121 gtgagccgcg accgaagcac ctctattggt tggcgaccgg cgtcgactcc atcgtcgtca
      181 ccggcgtcga tgaacacacc ctgcgcctgc gaccgacgcg cggcttcttg gcgagctcga
      241 gccaacgcat gttgcggagc gctgccagcg gcttcgagcg cgaccaagaa gttcgcctcg
      301 aggattgttc gatccgagtc gaagagctga cgctcgaccg ccgcccgcaa gccatcttgg
      361 tgcgcttccg ttcgaatctt gacgaccgcg cactcgcctt catgacctgg caggggccag
      421 gctatgtcgc gttcgtgccg cccgcgcccg gacagagcgt cgtcatcccc cgcgtcgatt
      481 tgtggaaggc gctattcggc tgagccaaac agggcttgaa gccatgccaa gagccatgcg
      541 tgagcagtcg actcatcgcg gctcagcgcc gggtgaagct gcagcgtgag agcgagccgg
      601 ccttcataag tcaggcatgc ggcgcccacc ggccgcgatt tgatcacggg attgaacgcc
      661 atccagaact cccgactctg cggtgagaat tccgaaccgc tcactcgcag cgagccgaga
      721 ttcgagaacg atccgtgctt tttgatgcgc gattcacgct gtgccaagcg gcggatgctc
      781 tcggggtcca agtgctcgag cagttgcaac agctgccaga cgccgaagtg ggccaacgcc
      841 tcgcgctcgc gcttgagcgc ctcgtcgacc tgcgcgacgt tcgccccggc cgggaaggcg
      901 acgtcgaacg ttgccgcctg gttggcggta tcacgcagct cgggtcgaag cccccgcatg
      961 ttcacgggga caatccaggc gacgctgccg cggtcgcgtt gcaggtgcgg cagcatggct
     1021 cgagtcaggc tccacagcat ccaagcattc agcgagacgc cgcgtgaacg cgccgcttgc
     1081 ctgagctcgt gggtgtcgct ctcgctgagc agcgcccacg cgtacgcgct cggtgccgtc
     1141 ggttgtcgag ggtccccggt ccaggcgtcg tcgaaatcgc gaaacggaat ggcttcgtgt
     1201 gagcgccgca gggcaaagcg cgctgccgcc aaccagcgcc gcgcgcggct cgggtagggc
     1261 tccgccagcc gcggcatatc gagggatttg ccctccagct ggcgcaacac gacggcgaga
     1321 ccgccgattc cgtcgtagtc ctggtgcggc aggaagtgcc acgtcggatg cgagcgctga
     1381 tcgtcgcagc ggcccagtcg aaatcgcatc gattcaccct cggcttcgag cgccgagaac
     1441 cactccgcaa agagatccgt gcggccgcgg actggtcttg gggcgaggtt tgccatcgtc
     1501 ctccacgtac gtgcggttgg cgatggaagt cgggtgactt caaattcgac aattggccta
     1561 cccgtcattc gggcgagcgc gcacctgaac cccatgactg cgctccccgc gttcgatgcg
     1621 gatcagctgt tcgatgtcca ggctccggac gagactcctc tcgattggcc gggaccgggt
     1681 ccgatcgacc gcgcagtgca cgacttgccc cacgcatcat ctacgctcga gtggtggtat
     1741 ttgaactgcc acctcgtgac ccgcgaaggc cgcgaagtgt cgctgttcgc cgcgttcttt
     1801 cgggagcgag ccggcgtgga tccggagacc ggagccgcta tttacgcgca ttcggtggcg
     1861 tgggcgctca gtgaaccgga tcgcgctcgc taccatccaa aggtcgcggt cgatccgcgc
     1921 gccccggcgc tcggcctcgc gaagctcgat gcagcgcgcc acgtgagcag tggccgcatc
     1981 gatcgggcgc tgcgcgaagt gctcgagcgc ggacgggtgc ccgggccaac acggctgctc
     2041 gattcacctg ccgtcgtttc gcagtcgctg ctgcacctcg actacggtcg cgaccgactc
     2101 gtcaagatcg atgcagacga atacgaactt tctctctacg acgaactcgc cgaaatcggc
     2161 tgctcgctcc gcttcgttcc acgcaagaac ccggttcgct acggaagcga cggtatcgtt
     2221 cacggcgtgc aagacgagcg gatgttctac tacttcatcc cgcgctgcga cgtgcgaggg
     2281 caactcacga tcgacggctc gagcgtcggc gtcgaccagg gtagcgggtg g
//
LOCUS       DASTKS010001064         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1730832, whole genome shotgun sequence.
ACCESSION   DASTKS010001064 DASTKS010000000
VERSION     DASTKS010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730832"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VFQ35_22435"
     CDS             <1..1391
                     /locus_tag="VFQ35_22435"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9933487.1"
                     /translation="VQMWSTGEYIHAPDWYRNFRYAEESARGLDCDEDLATPGELRFE
                     LSDSPALWLLAAGPTPLESHDADALVRQEEARRAAFRNSLLRAAESYLVARGDGRTII
                     AGYPWFSDWGRDTFIALRGLCITTGRLSEARSILLEWADAVSQGMLPNRFADRSEDEP
                     EFNSVDASLWYVVSASEFLDVDAKKRVLSASERATLESALRSIVLGYARGTRFGIRSD
                     TDGLLRAGAPLSQLTWMDAKVGQHVVTPRSGKPVEVQALWLNALHVTRRLSSEFSSLL
                     ERAQYSFVQRFWNPSSGALYDVVDVNHEPGTVDPSLRPNQIFAAGGLPYTPLAREQAK
                     SVVDVVERALWTPLGLRSLDPASSNYRPRYEGDIWSRDTAYHQGTVWPWLLGAFVEAW
                     VRARGGTREVKREARSRFLLPIEVHRSRAGVGHVSEIADAEWPFTPRGCPFQAWSLAE
                     LIRLEFGILADG"
     gene            1640..3634
                     /locus_tag="VFQ35_22440"
     CDS             1640..3634
                     /locus_tag="VFQ35_22440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933488.1"
                     /translation="MTERPKSEQSIEFERLALEGGDVYRQILDAIADMVLVKGPGSRI
                     LWANRAFCEYYGMTNEELANIIDAPFSPRDNTARFLRDDAYVFTTKQVLDIPEEPVTR
                     HDGLIQVFHTVKSPVLDQRGEAALTVGVSRNITERKRVKEDLARYREHLEGLVEARTR
                     ELRSASDRLQTIVASLVEGIVAVDAEGRVQLLNPRAEALLGTTSSAALGQPLRALLRL
                     YNEATEVAEGAPEFVEFDRVLTAQRPIVGVLQPEEGLRVLVSVTSSPLLSADQSPLGS
                     VVVLRDIGLEREVEQQRLRQQKLESLGVLAGGIAHDFNNILMGILGSLSIARLRLDSR
                     EEVGGILEQAEQACLRARTLTTQLLTFAKGGAPVKRVLFLRGVARDSAELALRGSNAR
                     LEFSCVEPLSAIEGDEGQLTQVISNLVLNAQQAMPDGGTVFLRLDTVELAQALGSLAT
                     GRYVRVEVEDTGCGIEPQNLSRIFDPYFTTKPSGNGLGLASVHSIVQRHDGHVAVDSQ
                     LGRGTRFTVYLPAVEPSAVEPSRPAGSEALRSRRVLVLDNDEMIRNVVTPMLERLGHT
                     VVAAGSSAEAIAAFDSARAQGGPFEVVLVDLTMPGDLGAGAVIERLRALDPSAKFIVM
                     SGYSVDPLRAESQRLGLVGTLQKPFTIEALAHMLARCARD"
     gene            complement(3604..5439)
                     /locus_tag="VFQ35_22445"
     CDS             complement(3604..5439)
                     /locus_tag="VFQ35_22445"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9933489.1"
                     /translation="MKRLDAYQLANLPLSTRVVASFALILGLALFWSGWAVSVFFVDW
                     EVTLLGVLSWGHRLMGLPQALLRALFAGHVASLLLLFGVARALFRRAPLPRLTRRALF
                     GLASALALADLACWLLVASSAVARSFLGAAVTLLGGLLTYFVASPLRAMWRRRSWKYD
                     GGEPVRVVVVGGGFAGLYAALGLDGALGYHPKLELTVIDRKNYFLFPPLLPSVAAGAI
                     ETRQVTYPFRRLFEATNVRFKKETVVAIDVEHRVIRSRVDVDEHVSSGALVARHDETP
                     FDYLVLAPGSITNSFRTEGIEHAFFMRELSDAIALRNHVIDCFETAARERDPNLVAEM
                     LRFVIVGAGPTGVEVASELRDLVDQVLLRRYPEIDPALVEVIVVDPGERILNGWDPRV
                     ANDASAQLTKIGVRIQRGGRVVKLGADWIAFNDGTKLETRTCVWCAGVSPSELLRACG
                     LVLNKSGRAEVGADCRAVGHDRIFVLGDAAQFPTADGRGLPPLGQVAFQQGAHTAKNL
                     VRLLRGEAPLDFRYFDFGQLVSVGHHFAAVKLVGVRLSGFLAWFVWRTLYLGKLVGFG
                     NKLRVVLDWTLDLFIERSISQLHTERHRHPRPAPTLQSRAQRASM"
BASE COUNT         1003 a         1758 c         1893 g          967 t
ORIGIN      
        1 cagtacagat gtggtcgacc ggtgaataca tccacgctcc ggattggtac cgaaactttc
       61 gctacgccga agagagcgcg cgcggcctgg actgcgacga agatctagcg acgcccggtg
      121 agctccgctt cgaactttcg gacagcccgg cgctttggtt gctcgcggcg gggcccacgc
      181 cactcgaatc gcacgacgcc gacgcgctgg tgcgccaaga ggaagcgcgc cgcgccgctt
      241 ttcgcaactc gctactgcga gcggcggagt catatctggt ggcgcggggc gatgggcgaa
      301 cgatcatcgc cggatatccg tggttctccg attggggccg ggacaccttc attgcgttgc
      361 gtggcctgtg catcacgacg gggcgcctgt ccgaggcgcg ctcgattctg ctcgaatggg
      421 ccgacgccgt ctcgcaagga atgttgccga accgtttcgc cgatcgcagt gaggacgagc
      481 ccgagttcaa ctcggtcgat gcttcgctct ggtacgtcgt ttcggcgagc gaattccttg
      541 acgtcgacgc gaagaagcga gtgctttccg cttctgagcg cgcaacgctc gagtccgcgc
      601 tccgctccat cgtgctcgga tacgcgcgcg ggacgcgctt cggcattcgc agcgacacgg
      661 acggtctgtt acgcgcggga gcgccgctgt cccagttgac ctggatggac gcgaaagtcg
      721 gtcaacacgt ggtgacgccg cgctccggta agccggtcga ggtgcaagcg ttgtggctga
      781 atgcgctgca cgtcacacgg cgcctttcga gcgagttctc gagtctgctg gagcgcgcgc
      841 agtatagctt cgtgcagcgt ttttggaacc caagttcggg tgcgctgtac gacgtcgtcg
      901 acgtgaacca cgagcccggc acggtcgacc cttcgctccg cccgaaccag atcttcgcgg
      961 cgggaggtct cccgtacacc ccgctcgctc gagagcaggc aaaaagcgtc gtcgacgtgg
     1021 tcgagcgagc cctctggacg ccgctcgggc tgcgttcgct cgatccggcg agttcgaatt
     1081 accggcctcg ctacgagggt gacatttggt cgcgcgacac ggcatatcac cagggcacgg
     1141 tctggccctg gctgctcggt gccttcgtcg aagcgtgggt gagggcaaga ggcggaacgc
     1201 gagaagtcaa gcgcgaagcg cggtcgcgct ttctgcttcc gatcgaggtt caccgttcgc
     1261 gcgccggggt cgggcacgtc tccgaaatcg ccgatgcgga gtggcccttc accccccgag
     1321 gctgcccgtt ccaggcctgg tcactcgcgg agctgattcg cctcgagttc gggatcctcg
     1381 ctgacggctg agctcgaatc ctgattgtgg cgaagttgtc gagtcgaaaa gaaccgaaac
     1441 cgtcctccgc tgcgtcactg tccctcgcgt caccgtgagg ggttagaaaa gcaggccaaa
     1501 aaaatgcgcg cctgggatcc cggatgggat cccggatgaa tcgggcgcct tttgagaacg
     1561 cgccgattgt cggatgtgtt actgtgcaaa atagtgccct cgcttgggtt tggagcgaga
     1621 ggcgcccaac cggcttcacg tgacagagcg tcccaagagc gagcaatcca tcgagttcga
     1681 gcgcctggcg ttggaaggtg gggacgtgta tcggcaaatc ctggacgcca ttgccgacat
     1741 ggtgctggtg aagggtccgg gctcgcgcat cctctgggca aatcgcgcgt tttgcgagta
     1801 ctacgggatg acgaacgagg agctcgcgaa catcatcgac gctccgttca gcccccgtga
     1861 caataccgct cggttcttgc gtgacgacgc ctacgtcttc acgaccaagc aagtcctcga
     1921 tatcccggag gagcccgtca cgcgacacga cgggctcatt caggtctttc ataccgtcaa
     1981 gtcgccagtg ttggatcagc ggggtgaagc ggcgctcacg gtcggtgttt cgcggaacat
     2041 caccgagcgc aagcgcgtca aggaggacct tgcgcggtac cgtgagcacc tcgaagggct
     2101 cgtcgaagcg cgcacgcgag agttgaggag tgcctcggac cggctgcaaa ccatcgtcgc
     2161 gtcgctggtg gaggggatcg tcgcggtgga cgccgagggc cgagtgcagc tcctcaatcc
     2221 gcgcgccgag gctttgctag gcactacgag tagcgcggct ctcggccaac ctttacgcgc
     2281 gctactccgc ttgtacaacg aggctaccga ggtcgcggag ggcgcgccgg agttcgtcga
     2341 gttcgaccgg gtgctcaccg cacagcgccc gatcgtcggt gttcttcagc ccgaagaggg
     2401 tcttcgagtt ctggtctcgg tgactagctc tccgcttctc tcggcggacc agtcgccgct
     2461 cggctcggtc gtcgtgcttc gagacatcgg cctcgagcgc gaggtcgagc agcagcggct
     2521 ccggcagcaa aagctcgagt cgctcggggt gctggccggc gggatcgcgc acgatttcaa
     2581 caacatcttg atggggattt tggggagcct ttccatcgcg cgcctgcggc tcgactcgag
     2641 ggaagaggtg ggcggcatcc tggagcaagc cgaacaagct tgcctgcgag cgcgcacgct
     2701 cacgactcag ctcttgacct tcgccaaggg cggcgccccg gtgaaacgcg ttttgttctt
     2761 gcgcggtgtc gcccgcgact ccgcggagct cgcgttgcgt ggctcgaacg cacggcttga
     2821 gttcagctgc gtcgagccgc tctcggccat cgaaggagac gaggggcagt tgacgcaggt
     2881 catcagcaac ttggtattga atgcgcagca ggccatgccc gacggcggca ccgtgttctt
     2941 gcgcctcgac acggtcgagc tcgcgcaggc cctcggttcg ctcgccacgg gacgctacgt
     3001 gcgcgtcgag gtcgaggaca cggggtgcgg catcgagccg cagaaccttt cgcgcatttt
     3061 cgacccctat ttcacgacca agccgagcgg aaatggcctc ggcctcgcgt cggttcactc
     3121 catcgtccag cgtcacgacg gtcacgtggc cgtcgactcg cagctgggcc gggggactcg
     3181 cttcacggtg tacttgcctg ccgtggagcc atccgcggtg gaacccagcc gacccgccgg
     3241 gagcgaggcg ttgcgctcgc ggcgagtgct cgtgctcgac aacgacgaga tgatacggaa
     3301 cgtcgtcacg cccatgctgg agcgcctggg tcacacggtg gttgcggcgg ggtcgtcggc
     3361 cgaagcgatt gcagctttcg actcggcgcg cgctcagggc gggccgttcg aggtggtctt
     3421 ggtcgatttg acgatgccgg gcgatctcgg cgcgggcgcg gtgatcgagc ggttgcgggc
     3481 gctcgatccg tccgcgaagt tcatcgtgat gagtggttac tcggtcgatc ctcttcgagc
     3541 cgagagccag cgcctgggct tggttgggac gctccagaaa ccgttcacca tcgaggcact
     3601 cgctcacatg ctcgcgcgct gcgcacgcga ttgaagggtg ggggcagggc gaggatgccg
     3661 gtgccgttcg gtgtgcagct gcgagatgga gcgttcgatg aacaagtcga gcgtccagtc
     3721 gagcacgacg cgcagcttgt tgccaaaacc aacgagcttg ccgagataga gcgtccgcca
     3781 cacgaaccac gccaagaagc cgctcaggcg aacgccgacg agctttactg ccgcgaagtg
     3841 gtgcccaacg ctgacgagct gcccgaagtc gaagtagcgg aaatcgagcg gagcttcgcc
     3901 gcgcaggagc cgcacgaggt tcttcgccgt gtgcgcgccc tgctgaaagg cgacttgtcc
     3961 gagcggcggt aggcctcgcc cgtctgcggt cgggaactgc gcggcgtcgc ccaaaacgaa
     4021 gatccggtcg tgacccacgg cgcggcagtc ggcgccgacc tcggcgcggc cgctcttgtt
     4081 caaaacgagg ccgcacgcgc gcaagagctc ggaagggctc acaccggcgc accacacgca
     4141 agtgcgagtc tcgagcttcg tgccgtcgtt gaaagcgatc caatcggcgc cgagcttaac
     4201 gactcgcccc ccgcgctgga ttcgcactcc aattttggtg agctgcgccg aggcgtcgtt
     4261 cgcgacgcgt ggatcccatc cgttcaagat gcgttcgcct gggtcgacga cgatgacctc
     4321 gacgagcgca ggatcgatct ccgggtaacg ccgcaacagc acttggtcga ccaagtcgcg
     4381 gagctcggag gcgacctcga cgccggtcgg gccggctccg acgatgacga accggagcat
     4441 ctcggcgacg aggttcgggt ctcgctcacg tgcggcggtc tcgaagcaat cgatgacgtg
     4501 atttctgagc gcgatggcgt cggagagctc acgcatgaaa aaagcatgtt cgatgccttc
     4561 cgtgcggaac gagttcgtga tcgagcccgg cgcgagcacg aggtagtcga agggcgtctc
     4621 gtcgtgtcgc gcaacgagtg caccgctgct cacgtgttcg tcgacgtcga cgcgcgagcg
     4681 gatgacgcgg tgctcgacgt cgatcgcgac caccgtctcc tttttgaagc gcacgttggt
     4741 tgcctcgaac aagcgccgaa aggggtaggt aacctggcgc gtttcgatgg cgccggccgc
     4801 gacgctcggc aagagcggag gaaacaagaa gtagttcttg cggtcgatca ccgtgagctc
     4861 gagctttggg tgatagccga gcgcgccatc gagcccgagc gcagcgtaga gaccggcgaa
     4921 gccgccgccc acgaccacga cccgaaccgg ctcccctcca tcgtacttcc aagatcggcg
     4981 gcgccacatc gcgcggagcg gcgaggcgac gaaataggtg agcaacccac cgagcagcgt
     5041 gaccgctgcg ccgaggaagg accgagcgac cgccgaactc gcgacgagca accagcacgc
     5101 gaggtcggcg agcgcgagcg ccgaagcgag cccgaagagc gcgcgtcgag tcagccgtgg
     5161 caatggagcg cgtcgaaaca gcgcgcgcgc gaccccgaac agcagcaaca acgaggctac
     5221 gtgacccgcg aacaaggcgc gaagcagagc ttggggcaag cccatcaggc ggtgtcccca
     5281 actcagcacg ccgagcagcg tgacctccca atctacgaag aagacgctca cggcccagcc
     5341 ggaccagaat agagcgagcc ctaagatgag tgcgaagctc gcgaccacgc gagtcgatag
     5401 tgggaggttc gcgagctgat aggcgtccag tcgtttcatg ttcgttcccg gggcgagtga
     5461 cgcgcgtcgc gcgtccgact cccgctacgg agctcgcccc gcgaggttac cgggccgccc
     5521 ggtcggcgcg gattttcgcg gatttgtccg caacttctcg aaaaagaccg gcgccgaaga
     5581 aaccgtccgg gggtcgcgac gtacgaagca acacacggcg g
//
LOCUS       DASTKS010001065         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1730874, whole genome shotgun sequence.
ACCESSION   DASTKS010001065 DASTKS010000000
VERSION     DASTKS010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730874"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..583)
                     /gene="hoxE"
                     /locus_tag="VFQ35_22450"
     CDS             complement(65..583)
                     /gene="hoxE"
                     /locus_tag="VFQ35_22450"
                     /inference="COORDINATES: protein motif:HMM:NF005747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxE"
                     /protein_id="HET9933490.1"
                     /translation="MAQPQRAPAATKHPSGDRRFEMLDTSMKRLRFQPDSLIEILHTA
                     QELFGHLRPDLLMYVARGLKLPPSRVYGVATFYHLFSFAPQAEHSCVVCTGTACYVKG
                     ADAILPAVEKQLGIRAGRSTADGKVSLSIARCLGACGLAPIVVYDGQVAAHQSEETSL
                     QTIQRWYSNGPR"
     gene            complement(587..1267)
                     /gene="hypB"
                     /locus_tag="VFQ35_22455"
     CDS             complement(587..1267)
                     /gene="hypB"
                     /locus_tag="VFQ35_22455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET9933491.1"
                     /translation="MCDVCGCGDPKLVRVDVHQSLLLHNDHDAAHNREHFQRAGVLAL
                     NVMGSPGSGKTSLLEATARRLGAERRIAALSGDLATDNDARRLAAAGIRAHAITTGSA
                     CHLSAELVHEGLHALGDVRDYDLLFVENVGNLVCPAIYDLGQRANVVVLSVTEGEDKP
                     LKYPVMFRRADLVVLTKIDLLPHLDVQVGAIETALAQVMPTPRLLQVSARTGEGVDRF
                     VHWLSNLH"
     gene            complement(1260..1604)
                     /locus_tag="VFQ35_22460"
     CDS             complement(1260..1604)
                     /locus_tag="VFQ35_22460"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET9933492.1"
                     /translation="MHELSIVSRMLEQVDREIRNHRGARVRRVKVRIGAASGVEAELF
                     EHAFVQLRSGACETAELEVNRVPSCWRCSACQREFGSGDALFCECCGLPCVLVSGAEL
                     MLDRIELELSHV"
     gene            complement(2217..2615)
                     /locus_tag="VFQ35_22465"
     CDS             complement(2217..2615)
                     /locus_tag="VFQ35_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9933493.1"
                     /translation="MGTIVRKPAARATGSRAKRKERGDSSQTRCTIETTAGLIGGRWK
                     AVVIFHLLAGTRRFGELRRLLPNATARMLTLQLRELEADGLVRRKVFAEVPPRVEYTL
                     TEEGASLGPIVTAMGVWGKAYERRSRHGSK"
     gene            2721..3704
                     /locus_tag="VFQ35_22470"
     CDS             2721..3704
                     /locus_tag="VFQ35_22470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET9933494.1"
                     /translation="MAELSTNRALRSKAVAYTGVGGLEVIQVVEREVRAPAANEVRVE
                     VKAAAVSPTDLLLRDPNFRSNLVAPAAPPTLLVPGMDLAGVVEAVGPGVSRLRPGYKV
                     MAVVWPRRPEGGAYAQHVVAPAASVVRIPEGLSFAQASTLPMNGLTALLALELAALER
                     GQVLAVSGGAGLLAHFAIAAAKRQGIKVIADARASEKALVESYGANIVVERGADFAGA
                     IRKELPSGADALLDTAVLGEKSFGALRDGGVYIPVRGWGDQPAERAIQIKPVFVFGVL
                     KRTEWLEQLSDWVSRGYIEPLVAGEYAPEQAGDAQNALAAGGLRGRPVIVF"
BASE COUNT          690 a         1234 c         1257 g          697 t
ORIGIN      
        1 cgtgcagcag cgcagccgca ccggtttgcg ctgagagagc tccttgtcgc ctaacgcgcg
       61 cagttcatcg aggtccattg gaataccacc tctgaatcgt ctgaagtgac gtctcttcgc
      121 tctgatgagc ggcgacctga ccatcgtaga cgacgatcgg cgcgagcccg cacgctccga
      181 ggcaacgcgc gattgagagc gacaccttgc cgtccgcggt ggagcgaccg gccctgatgc
      241 cgagctgctt ctcgactgcg ggcaaaatcg cgtcggcccc tttcacgtaa caagcggtgc
      301 ccgtgcacac cacgcagcta tgctcggcct gaggcgcgaa ggagaaaaga tggtagaacg
      361 tcgccacccc gtagacacgg ctcggaggta gcttgagacc tcgcgccacg tacatcaaga
      421 gatccggccg caaatggccg aacaactctt gagctgtgtg cagaatctcg atgagtgagt
      481 cgggttggaa gcggagccgc ttcatgctcg tgtcgagcat ttcgaagcgg cggtccccgc
      541 tcgggtgttt ggtagcagct ggagcgcgtt gtggctgggc catgattcaa tggaggttcg
      601 agagccagtg gacgaatcgg tccacacctt cgccggtgcg cgccgaaact tgcagcaagc
      661 gcggtgtggg cattacctga gcgagggcgg tttcgatcgc gcccacttgc acgtcgaggt
      721 gtggcagcaa gtcgatcttg gtgagcacca ccagatcggc gcgacggaac atgaccggat
      781 acttgagtgg cttgtcctcg ccttcggtca ccgagagcac caccacgttt gccctctgcc
      841 ccaagtcgta gatcgcaggg cacaccaaat tgccgacgtt ttccacgaag aggaggtcgt
      901 agtcgcgaac gtcgcccaag gcgtgcagcc cttcgtgcac gagctcggcg ctcaagtgac
      961 aggcggaacc cgtggtgatg gcgtgcgcac ggattcccgc agcagccagg cgacgcgcgt
     1021 cgttgtcggt cgccaggtcg ccgctcagcg cggcgatcct tcgttcggcg ccgagccgtc
     1081 gcgccgtcgc ttccaacaac gaagttttgc ccgagcccgg cgagcccatc acgttcaggg
     1141 ccaacacccc cgcgcgctga aagtgctcgc ggttgtgcgc agcgtcgtga tcgttgtgca
     1201 aaagcaggct ctggtgcacg tcgacacgga cgagcttcgg atcgccgcag ccacacacat
     1261 cacacatgag aaagctccag ttcgatgcga tcgagcatca gctcggcgcc ggataccaac
     1321 acgcagggaa gcccgcaaca ctcgcaaaac aaggcatcgc cgcttccgaa ctcgcgctga
     1381 caggcgctac agcgccagca cgaggggacg cggttcactt cgagttccgc cgtttcgcaa
     1441 gctcccgagc gaagctgcac gaaggcgtgt tcgaagagct cggcctccac gcctgatgcg
     1501 gcgccgattc gcactttgac ccgtcgtacg cgcgcgccac gatgattgcg gatttcgcga
     1561 tcaacctgtt cgagcatgcg gcttacaatc gaaagctcgt gcattgggcc tcaacgacga
     1621 cgaccaacgg ctcgcatctt agtgccaagt tgggtgaact ctgaagcact tttgaagcac
     1681 ttttggcgcg atgatatctg tcatgacgag acatgtcggt taactcatgg ctgaaacccg
     1741 acggttgccc tctcgccttc tagagcttgc gctcacgccg gcgttgcttt gcctttgctt
     1801 tggctcttgc gagggccttc ttcagaggct tcggcgcccg ccagcgtgct gacgtggctg
     1861 tcttcgcgca gtccttcgct tgacaacggt cgaggcttgc aggtcgaagt tgtagtgccg
     1921 acgcaaagac cgagcgcgct cagcgccagc gcagccccga ttccgcggtg ccgcagacga
     1981 gaagaatggg agatggtctt ggtgggtctc gaagatctta gcgcgaatcg ccgctacgaa
     2041 gcgcccgcgc agtccgcgcg cacgtgcaca gagcggctag gtcaccaggg caggcagcgt
     2101 cgcgttcgga tatctcgatt tgcgacactc cggcgttcac gacgtcgcgt ggaccgggac
     2161 gatgccgaga agcagacccc gcctgagtga gacacgccgt agctctcgga ttactcctac
     2221 ttcgagccgt gacgtgaacg acgctcgtac gcctttcccc acacgcccat cgccgtcact
     2281 atcgggccga gactcgcgcc ctcctccgtc aacgtgtact cgacgcgcgg aggcacctcc
     2341 gcaaagacct tacggcgaac caagccgtcg gcctcgagct cgcgcagctg aagcgtcagc
     2401 atgcgagcgg tcgcattggg cagaagacgc ctgagctctc cgaagcggcg cgtaccggcg
     2461 agcaggtgaa agatcacgac cgccttccag cgaccaccga ttagccccgc ggtcgtttcg
     2521 atcgtgcagc gggtctggct cgaatcgccc cgctcctttc gcttggcgcg gctacctgta
     2581 gctcgagcgg ccggctttcg tacgatagtt cccatttggg tagtatgtat gatttatgtg
     2641 cgtacttgcg agtattcgcc tatgtcctag gttgctcagc gggaacgagg cggtcgtttc
     2701 gtcaccgcag aggagaagac atggcggaac tttcgacaaa tcgagcgctc aggagcaagg
     2761 ccgtcgcgta caccggtgtc ggcggcctgg aggtaatcca ggtcgtagaa cgcgaggtcc
     2821 gggcgccggc ggccaacgag gttcgcgtcg aagtaaaggc ggccgcggtc agcccgaccg
     2881 acctgctgct tcgtgacccg aactttcgct cgaacttggt agcgcccgcc gctccgccca
     2941 cgctcctcgt gccgggcatg gacctcgcgg gcgtcgtcga agcggtcggt ccaggggttt
     3001 cgcggctgcg ccctggatac aaagtgatgg cggtggtttg gccgcggcgt ccggagggcg
     3061 gcgcgtatgc gcagcacgtc gtagcgccgg cggcctcggt agttcgcatt cccgagggcc
     3121 tctctttcgc ccaggcgtcc actctgccga tgaacgggct cactgcgctg ctcgcgctgg
     3181 agctcgcggc gctcgagcgc ggacaggtcc tggcggtgag cggcggcgcc gggctcctcg
     3241 cgcacttcgc gatagcggcc gcaaagcgtc aaggaatcaa ggtcatcgcc gacgccaggg
     3301 cgtcggagaa agccttggtc gagagctacg gcgcaaatat cgtggtcgag cgcggtgccg
     3361 acttcgccgg ggcgatccgc aaggagctgc ccagcggtgc cgacgcgctg ctcgacaccg
     3421 ccgtcctcgg cgaaaagagt ttcggcgcac tacgcgacgg tggtgtctac attcccgtgc
     3481 gcgggtgggg agaccagccc gccgaacgcg cgattcagat caagcccgtc ttcgtattcg
     3541 gagtgctcaa acgtaccgag tggctcgagc agctcagcga ctgggtttcc aggggctaca
     3601 tcgagccgct ggtcgccggc gaatacgcac ccgagcaagc gggtgacgcg cagaacgcac
     3661 ttgccgctgg tggcctgcgc ggtcggcccg tcatcgtctt ctaggtcgcc tcgggttgtc
     3721 gggagcgtcg gtgccggcgt cgtcacggcg ccgacgccgc gcgtggtgct tccgtcgtcg
     3781 caaagaagtc gaggcgccgc tgcgctttgg ccggcgcgga ccaggggacc acggcgaccc
     3841 gaggcgcgag cgcggccacc tcggcgtgaa agcgggcc
//
LOCUS       DASTKS010001066         6738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1285095, whole genome shotgun sequence.
ACCESSION   DASTKS010001066 DASTKS010000000
VERSION     DASTKS010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285095"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..5678)
                     /locus_tag="VFQ35_22475"
     CDS             complement(183..5678)
                     /locus_tag="VFQ35_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HET9933495.1"
                     /translation="MPDSQAQASARSLEGGSYEVIRKRLLERAAELGNQAEALNSRRK
                     QLFGGSELALIATERVRTENNCVPRDVVSVGGSMLFGFEVFIGLKSETRVSDVLSLYR
                     FEKLEGGYDLSPAPFEGPSAFLADPEFDKQFRDSFRYAKEARLLQLVRTETRLLVVLQ
                     IGASVADHKVLRFSIDGKGTIAFIDARGEEDYKAPRPHAFEWTLTTRENHVAGPHPHV
                     NVLNEVFVETVGGDLTIKVENNTQDGLGVYREAVEDPNQTLDDGEVAYAKVAGLILLR
                     VKPFREKGPRYLIFNPRNQHVLRVDALGAACVALPDDHGVIFPGGYYLSSGEHKLFDG
                     EASGIELETVVPSPNGEDVLYVFYRPLEGEYLLYPYNLIEKEVKNAIRCHGYSLFADG
                     TMLVFRAQEEPTRVHPVQIWRTPFTTSEFAASAPRDGSYLAKVGNADLVAGISELFSI
                     KKLINADGPTRRTYEELISALRRVVDKHYWLGNDEAFGLLGIVRELIKTCELVVDEFE
                     KQLALERTAREAVDKAGQAQKALLEKVRPTELRSVEDFLSSLSQLKHQRGHLISLKDL
                     RAVDVAAIERLEREIAESFTDVSRACVDFFLAENAFQPLLDRLEKLLASVGDVQKTAD
                     LAPAQKELDAVHEGLTLLSETIGNLPIEDPTVRTKILDGTSSAFAQQNRTRAVLDARR
                     KELAAHEGRAEFGVEFKLLGQSVTSALALSTTPEACDEQLAKLLLRLEGLEARFGELE
                     EFSSKLAEKREELMDAVSTRRTQLVEERQRRAQNLASAGQRILSGVARRVQTLTSADE
                     LNAYFASDAMVQKLDELTEKLRELGDSVRADELEAKLKSEKQNALRALRDREDLLEDG
                     GNVIRFGKHRFSVNTRPLELAIVPREGGLNTHLTGTDFFEPLSSPELDSAKELWEQEL
                     VSETPQIYRGEFLAVSLFLDAEAGRAGTSLEDLRRALREERLDALVREAATQRFDEGY
                     ERGVHDVDATAILSRVLESERDAGVLRYSPRARAAAWLFWQGLPRERRELVQKQAESA
                     VRLRARLGDHSAEFELARTLASGLADELHVLGLEGEISVAPFAARYLVREVGEESPRF
                     HESGHAEELRASFVSHLDESGALRGFEGDLRSLSKHRRERLAVALGYFDAFARRAGRG
                     GAERLEIAARLVCEGDLTFAVETQKTESRVENLLGSHPRIQQRVLEFELGELLERVGR
                     YIEAWAPRFRAYRTLRAELAQRERERLRLSEFMPRVLTSFVRNRLIDEVYLPLVGTNL
                     AKQLGAAGANKRTDQMGLLLLVSPPGYGKTTLMEYVASKLGLVFMKVNGPALGADVHS
                     LDPSEAPNATARQEVEKVNLALEMGNNVMLYLDDIQHTSPELLQKFISLCDAQRRIEG
                     VWRGKTRTYDLRGKKFCVVMAGNPYTESGARFQIPDMLANRADTYNLGDILDGKQEAF
                     ARSYLENALTSHPLLAPLAGRDPGDVHKLIAMAQGEPVTLGELSHSYSSAEAQEIVAL
                     LSRALKVQRVLLRVNQEYIASASRDDRYRNEPPFKLQGSYRNMNKLVEKLAPVMNDDE
                     LERLIDDHYVSEAQTLTQGAEQNLLKLGELRGRLGPDQAKRWEQIKQGFLRVQRMGGK
                     DDDPVARVTGTLSGLDTQLQGIREALVSAARAESEKPLPAPAVENPPTRVEVRLDGER
                     DAALVRLVAQQGELLKYALDAIARVGAARSPNGEFAEIKDAIVRLGEKLQHGVAGVRR
                     VDVDLAPSGPSNFYRPISSEDVCRDGGLFVATYEKPPPLGADVLVSVVFPTGPRCEFS
                     GSVAWCRDYLGDHAPAGFGVRFLELSDDARSLIHGYTDEREPMLWDD"
     gene            complement(5705..>6738)
                     /locus_tag="VFQ35_22480"
     CDS             complement(5705..>6738)
                     /locus_tag="VFQ35_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933496.1"
                     /translation="KARQLEVIGREREVALVGIARDREVEGQKKEIANVVRERIAVEK
                     SVASEEELIKDIRTVAQANREKEAVRIAAEAQATELSVKQVKAAQASEEVTKIKARER
                     VISADAELETADKGARAKIRLAEGVQAEVAAEGLAKARIKEADATASEKLGLVEARVA
                     LEKLTAQATGEEKQGLAKVRVREAEAAAVEKQGAAEATAIRERLVAEAHGLAQKAESM
                     KALDQSGRGHEEFRLRMEKERTVAIESLKAKKDIVSAQARILAEAMGHAKINIVGGDG
                     QFFQRFISAISLGQSVDGALEESESLRALVSELVGNGDGSPDLLGRALERLAQGTKPQ
                     IEASTPKRP"
BASE COUNT         1044 a         2286 c         2161 g         1247 t
ORIGIN      
        1 gtgcgaactc ggaggatcga tcttcccgag cggagacatt gcgccatcct cgaatcagcc
       61 tttcgggtgc tccgatggcg cacgctcaca cgcgaccgga gtgcacgttc gagacccaag
      121 cggaggacga ggtccgcgaa cgagaccact tcgtcgcacc tccggcgctt cgaccgtctc
      181 gctcagtcat cccacagcat cggctcgcgc tcgtccgtgt agccgtgaat gagtgagcgc
      241 gcgtcgtcgg agagttccag gaaccgcacg ccgaagcctg ccggggcgtg gtcgccgagg
      301 tagtcgcgac accaggcaac gctgccggaa aattcgcagc gcggcccggt ggggaagacc
      361 accgagacga gcacgtcggc gccgagcggc gggggctttt cgtaagtggc cacgaacaat
      421 ccgccgtcgc ggcagacgtc ctcggaagaa atcgggcggt agaagttgct cggacccgac
      481 ggggcgagat cgacgtcgac gcgccgaacg cccgccacac cgtgctggag cttctcaccc
      541 agtcgaacga tcgcgtcctt gatctcggcg aactctccgt tcggcgagcg agccgcacca
      601 actcgtgcga tcgcgtcgag cgcgtacttc aagagctcac cctgctgcgc gacgagacgc
      661 accaaagcgg cgtctcgctc gccgtccagg cgcacctcga cgcgggtggg cgggttctcc
      721 accgcgggag cggggagcgg cttttctgac tccgctcgag ccgcgctcac cagtgcctcg
      781 cgaatgcctt gaagctgcgt gtcgaggccg ctcagcgtgc ccgttacgcg cgccacggga
      841 tcgtcgtctt tgccgcccat gcgctgaacg cgcaaaaatc cctgtttgat ttgctcccag
      901 cgcttggcct ggtcggggcc gaggcgaccc cggagctctc cgagcttcag caggttttgc
      961 tccgctccct gggtgagggt ttgagcttcg ctcacgtagt ggtcgtcgat gagccgctcg
     1021 agctcgtcgt cgttcataac cggcgcgagc ttttcgacca acttgttcat gttgcggtag
     1081 ctgccctgca acttgaacgg cggctcgttc cgatagcgat cgtcgcgaga cgcggacgcg
     1141 atgtactcct ggttcacgcg caagagcacc cgctggacct tcagcgcgcg gctcagcagc
     1201 gcgacgatct cttgggcctc cgcgctcgaa tacgagtgcg agagctcacc gagcgtcacc
     1261 ggttcgcctt gcgccatcgc gatcaacttg tgaacgtcgc ccgggtcgcg gcccgcgagt
     1321 ggggcgagca gcgggtgaga ggtgagcgcg ttctcgaggt agctccgcgc gaacgcctcc
     1381 tgcttgccgt ccaggatatc gccgaggttg taggtgtcgg cgcggttcgc gagcatgtcc
     1441 gggatctgaa agcgcgcgcc gctctccgtg taggggttcc ccgccattac gacgcagaac
     1501 ttcttccctc gcagatcgta ggtgcgcgtc ttgccgcgcc aaacgccttc tatccggcgc
     1561 tgcgcgtcgc agagcgagat gaacttctgc aagagctccg gcgaggtatg ctggatgtcg
     1621 tcgaggtaga gcatcacgtt gttgcccatc tcgagcgcga ggttgacctt ctcgacctct
     1681 tgacgcgccg tcgcgttggg cgcttcgctc ggatcgagcg agtgcacgtc ggcgccgaga
     1741 gcggggccgt tcaccttcat gaacacgagc ccgagcttgc tcgcgacgta ctccatcagc
     1801 gtcgtcttgc cgtagccagg cggcgacacg agcagcagga gccccatctg gtcggtgcgc
     1861 ttgttggcgc ccgcagcccc gagctgcttt gcgaggttcg tgccgacgag cggcaggtac
     1921 acctcgtcga tcaagcggtt tcgcacgaac gaggtcagca cgcgcggcat gaactcggag
     1981 agacgtagcc gctcgcgctc gcgctgcgcg agttcggcgc gcagcgttcg gtaagctcga
     2041 aaccgcggcg cccacgcttc gatgtagcgc ccgacgcgct cgagcaactc gccgagctcg
     2101 aactcgagaa cccgctgctg aatccgcgga tgagagccga gcaggttctc cacgcgcgac
     2161 tccgtctttt gcgtttcgac cgcgaacgtg agatcgccct cgcagacgag gcgagccgca
     2221 atttcgaggc gctccgcccc gccgcggccc gcgcgccgcg cgaacgcgtc gaaatagccg
     2281 agcgcgaccg cgagtctttc ccggcggtgc ttcgacaggg agcgaaggtc gccttcgaac
     2341 ccacgtaacg cgccgctctc gtccaggtgc gaaacgaacg aggcgcgcag ctcttcggcg
     2401 tgaccgctct cgtgaaagcg cgggctctct tccccgactt ccctcaccaa gtagcgcgcc
     2461 gcgaacggcg cgacgctgat ctcgccctcg agcccgagta cgtggagctc gtcggcaagt
     2521 ccgctcgcca aggttcgagc caactcgaac tcggcggagt ggtcgccgag gcgcgcgcgc
     2581 agacgcaccg cgctttcggc ttgcttctgg acgagctccc ggcgctcgcg cgggaggcct
     2641 tgccaaaaga gccacgccgc cgcgcgcgcc cgcggcgaat acctgagcac gcccgcgtcg
     2701 cgctcgctct ccagaacgcg cgagagaatc gcggtcgcgt cgacgtcgtg cacgcctcgc
     2761 tcgtagcctt cgtcgaagcg ctgcgtcgcc gcctcgcgca cgagcgcgtc gaggcgctcc
     2821 tcgcgcaagg ctcgccgcaa atcttcgagc gacgtacccg cgcgcccagc ctcggcgtcg
     2881 aggaagagac tcacagcgag gaactctcct cggtagattt gcggcgtttc ggagacgagc
     2941 tcctgctccc agagctcctt cgcagagtcg agctccggcg acgacaatgg ctcgaagaag
     3001 tcggtgccgg tgaggtgggt attcaggccg ccttcgcgcg gcacgatggc gagctcgagc
     3061 gggcgcgtat tcacggagaa gcggtgctta ccgaagcgga tgacgtttcc gccgtcctcg
     3121 agcaggtcct cccggtcgcg cagcgcgcgg agcgcattct gtttctcgct cttcagcttc
     3181 gcctcgagct cgtcggcgcg caccgagtcc ccgagctcgc ggagcttttc ggtgagctcg
     3241 tcgagcttct ggaccatcgc gtcggaggcg aagtaggcgt tgagctcgtc ggcgctggtg
     3301 agggtttgga cccgccgcgc gaccccgctc aggatgcgct gacccgcgct cgcgaggttc
     3361 tgcgcgcggc gctgacgctc ctccaccagc tgcgtgcgcc gggtcgagac ggcgtccatc
     3421 agctcttcgc gcttctcggc gagcttgctc gagaactctt cgagctcacc gaagcgcgcc
     3481 tcgagccctt cgagccggag cagaagcttc gcgagttgct cgtcgcacgc ctccggtgtc
     3541 gtcgacagag cgagcgcaga cgttacgctt tgcccgagca gcttgaattc gacgccgaac
     3601 tcggcgcggc cttcgtgggc ggcgagctct ttacggcgtg cgtccagcac ggcgcgcgta
     3661 cggttctgct gcgcgaacgc cgagctcgtg ccgtccagga tcttcgtgcg gacggtcggg
     3721 tcctcgatgg gaaggttccc aatcgtttcg ctgaggaggg tcaagccttc gtggaccgca
     3781 tcgagctcct tctgagccgg ggcgaggtcg gccgtcttct gtacgtcgcc caccgacgcg
     3841 aggagcttct ccagccggtc gagcaacggc tgaaaggcgt tctccgccag gaagaagtcg
     3901 acgcacgcgc gactcacgtc ggtgaaactc tcggcgatct ctcgctcgag gcgctcgatc
     3961 gcggcgacgt cgaccgcgcg cagatctttc agagagatca ggtggccgcg ctgatgctta
     4021 agctggctca ggctcgaaag gaagtcctcg acgctcctga gctcggtcgg gcgtaccttc
     4081 tcgagcaggg ccttctgcgc ttgccccgcc ttgtcgaccg cttcacgcgc ggtccgctcc
     4141 agcgcgagct gcttctcgaa ctcgtcgacg acgagctcgc acgtcttgat gagctcccga
     4201 acgatgccga gcaggccgaa tgcctcgtcg ttgccgagcc agtagtgctt gtcgaccacc
     4261 cggcgcagcg ccgaaatcag ctcctcgtag gttcggcgag tggggccgtc ggcgttgatg
     4321 agcttcttga tgctgaagag ctcactgatc ccagcgacga gatccgcgtt ccccactttc
     4381 gcaaggtacg agccgtcgcg aggcgccgaa gccgcaaact cgctcgtcgt gaacggggtg
     4441 cgccagatct gcactgggtg gacgcgcgtt ggttcctctt gggcgcgaaa taccaacatg
     4501 gtgccgtctg cgaagaggct gtagccgtgg cagcgaatcg cgtttttgac ctctttctcg
     4561 atgaggttgt aggggtaaag gaggtattcg ccttcgagcg gacgatagaa gacgtagagc
     4621 acgtcttcgc cgttcggaga cgggacgacg gtctcgagct cgatccccga ggcctcaccg
     4681 tcgaacagct tgtgctctcc gctcgaaagg taatagcccc cgggaaagat cacgccgtgg
     4741 tcgtccggca gcgccacaca agccgcgccg agcgcgtcga cgcgcagcac gtgctggttg
     4801 cgcggattga agatcaaata gcgcgggccc ttctcgcgaa acggttttac gcgcagcaaa
     4861 atcaagcccg caaccttcgc gtacgcgacc tccccgtcat ccagggtttg gttcgggtcc
     4921 tcgaccgcct cgcggtacac cccgagccca tcctgggtgt tgttctccac cttgatggtg
     4981 agatcgccgc ccaccgtctc gacgaagact tcgttcaaga cgttgacgtg cgggtgcggc
     5041 cccgcgacgt gattttctcg ggtcgtgagc gtccactcga aggcgtgcgg gcgcggcgct
     5101 ttgtagtcct cctctccgcg cgcatcgatg aacgcgatcg tgccctttcc gtcgatcgaa
     5161 aagcgcagca ccttgtgatc ggcgacgctc gcgccgatct gcaagaccac gagtagccgc
     5221 gtctccgtgc gcacgagctg caggagccgc gcctccttcg cgtagcgaaa gctgtcgcga
     5281 aactgcttgt cgaactccgg gtccgcgagg aaggcggaag gcccttcgaa gggcgcagga
     5341 gacaaatcgt agccgccctc gagcttttcg aagcggtaga ggctgagcac gtcgctcacg
     5401 cgcgtctcgc tcttgaggcc gatgaagact tcgaagccga acaacatgct gccgccgacg
     5461 ctcaccacgt cgcgcgggac acagttgttc tcggtgcgaa cgcgctcggt cgcgatcagg
     5521 gcgagctcgc tgcccccgaa gagttgcttt cggcgagagt tcagcgcttc ggcttgattg
     5581 ccgagctccg ccgctcgctc gagcagccgc ttgcgtatga cttcgtaact accgccttcg
     5641 agcgaacgcg cggaggcctg tgcctgcgaa tcgggcatga ctcagctcgg attggcttcg
     5701 gatctcaggg gcgcttgggg gtgctcgcct cgatctgcgg cttggtaccc tgcgccaatc
     5761 gctcgagtgc gcggcccagc agatccggcg aaccatcgcc gttacccacg agctccgaga
     5821 ccagcgctcg taaggactcg ctctcttcga gggccccgtc caccgactgt ccgagcgaga
     5881 tggcgctgat gaaccgctgg aagaactgcc cgtcgccgcc cacgatgttg atcttggcgt
     5941 gtcccattgc ctccgccaag atgcgcgctt gcgcggacac gatgtccttc ttcgccttga
     6001 gcgactcgat cgcgaccgtg cgctccttct ccatccggag ccggaactct tcgtggcccc
     6061 gtccagactg atcgagcgct ttcatcgact ccgccttttg agcgagaccg tgcgcctccg
     6121 cgacgagccg ctcgcggatc gcggtcgcct ccgcagcgcc ctgcttctcg accgcagcgg
     6181 cttcggcctc gcgcacacga accttggcga gcccttgctt ctcttccccg gttgcctggg
     6241 cggtgagctt ttcgagcgcg acgcgcgcct cgaccaagcc aagcttctcg ctcgcggtgg
     6301 cgtccgcctc cttgatgcgc gccttggcga ggccctcggc ggcgacctcg gcctgcaccc
     6361 cttcggcgag gcggatcttc gcgcgcgcgc ccttgtcggc ggtctcgagc tccgcgtccg
     6421 cggaaatcac gcgctcgcgc gccttgatct tggtcacctc ttcgctcgcc tgcgccgcct
     6481 tcacctgctt cacgctgagc tcggtggcct gggcctcggc cgcaatgcga acggcctctt
     6541 tctcgcggtt ggcctgggcg acggtgcgga tgtctttgat gagctcctct tcggaggcga
     6601 cgctcttttc cacggcgatg cgctcgcgca ccacgttggc gatttccttc ttctgcccct
     6661 cgacctcgcg gtcgcgcgcg atgccgacca gggccacctc gcgttcgcgg ccgatcacct
     6721 cgagctggcg cgccttct
//
LOCUS       DASTKS010001067         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1955754, whole genome shotgun sequence.
ACCESSION   DASTKS010001067 DASTKS010000000
VERSION     DASTKS010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955754"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFQ35_22485"
     CDS             <1..219
                     /locus_tag="VFQ35_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PGRS family protein"
                     /protein_id="HET9933497.1"
                     /translation="KGGDGGAGGPGAGGNGGPSYAVVASGATPMKLKGTTLAVGSGGP
                     AGPGGAAGTVSGARGSTGVSAEQLALTP"
     gene            322..2097
                     /locus_tag="VFQ35_22490"
     CDS             322..2097
                     /locus_tag="VFQ35_22490"
                     /inference="COORDINATES: protein motif:HMM:NF006129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET9933498.1"
                     /translation="MSEAKVSDFVIDRLNAWGVDTIYGYSGDGINGLLGALGRASKRP
                     RFVQPAHEELCALMAAGHAKYNGGVGVCLVTQGPGAVHALNGLYDAKLDHQPVVALIG
                     QVAQSASGSYYQQEIDLVSLYKDVAHEHVQVLSTPEQALHVVDRAFRTALAERSVTAL
                     IIPHDVQQKPAPDGPKQKHGELASSVGYVEPCVVPQKVELERAAALLNAGKKVAILAG
                     AGALRATEEVLAVAEKLGAGVAKALLGKAAVPDDLPFVTGSVGWLGTRASNTMMKECD
                     TLLILGSQFPYTEFLPKPGSARAVQVDIDGKALGGRYPTEVNLGGDCAQTLRALLPLL
                     DTKNDRSFRARIEELVRDSRGELERVAKLDAHPLNPARVFWELNRVLPSNVIISGDCG
                     TSTLWYARYIDIRKGMLATLSGTLATMGSGIPYALAAKVGHSDRPVLAIVGDGAMQMN
                     GINSLITVAQHYRNWRDPRLVIVVLNNQQLSYVTWEQRVMEGDPKFAPSQDLMDFPFA
                     RYAELLGLEAARVANPDELADVYRRAFSAKRPFVVEAFTDPNAPPLPPELTEEQKKML
                     EQAFKQADPDVPAAKRLFDKFQSVR"
BASE COUNT          419 a          739 c          681 g          341 t
ORIGIN      
        1 aaaggcggtg acggcggcgc gggcggaccc ggtgccggcg gcaatggtgg accgtcgtac
       61 gccgtcgtcg cttccggcgc gacgccgatg aagctcaagg gaaccaccct ggccgtgggg
      121 agcggaggcc cggcgggacc gggcggcgcc gcggggactg tttcgggagc gcgcggcagc
      181 accggcgtga gcgcggagca actggcgctc acaccatgac cccgcgctcc accgctcgcg
      241 aacgatcgac ggttcgagca ctcgccaaca actagcgcac gcccggcagc gtacggttcg
      301 agcaaccgag aggaacgtcc cgtgtctgaa gcaaaagtca gtgacttcgt gatcgatcgc
      361 ttgaacgcat ggggcgtcga caccatctac gggtactcgg gcgacggaat caacgggctg
      421 ctcggcgcgc tcggtcgcgc gagtaagcgc ccgcgcttcg tccagcccgc gcacgaggag
      481 ctctgcgcgc tgatggccgc aggccacgcc aaatacaatg gcggagtggg cgtctgtctc
      541 gtcactcagg ggccgggagc cgtgcatgcg ctcaatggtc tctacgacgc caagctcgac
      601 catcagcccg tcgtcgcgct gatcgggcaa gtcgcccaga gcgcgagcgg gtcctactac
      661 caacaggaaa tcgatctcgt ctcgctctac aaggacgtcg cgcacgagca cgtgcaggtt
      721 ttgtcgacgc ccgagcaagc gctccacgtc gttgatcgcg cttttcgcac cgcgctcgcg
      781 gaacgcagcg tcaccgcgct catcattccg cacgacgtgc agcaaaagcc cgcccccgac
      841 ggtcccaaac aaaaacacgg agagctcgcg tcgagcgtcg ggtacgtcga accgtgcgtg
      901 gtgccgcaaa aagtcgagct cgagcgcgcc gccgcgctgc tcaacgcggg caaaaaagtc
      961 gccattttgg ccggagccgg agcgctacga gcaacggagg aagtgctcgc cgtggctgaa
     1021 aagctcggcg ccggcgtcgc aaaagcgttg ctcggcaaag ccgcggtgcc ggacgacctc
     1081 ccgttcgtca cggggtccgt cgggtggctc gggacccgag cgagcaacac gatgatgaag
     1141 gagtgcgaca ctctcctcat cctcggctcg cagttcccat acaccgagtt tctccccaag
     1201 cccggcagcg ctcgcgccgt gcaggtcgac atcgacggca aggcacttgg agggcgctac
     1261 cccaccgagg tcaacttggg cggtgactgc gcccagacat tgcgcgcctt gctgccctta
     1321 ctcgacacga agaatgatcg ctcgttccgc gcgcgcatcg aagagctggt gcgcgactct
     1381 cgcggcgagc tcgaaagagt cgcaaagctc gacgcgcacc cgctgaaccc tgcacgcgtc
     1441 ttttgggagc tgaaccgcgt tctcccttcg aacgtcatca tcagtggaga ctgcggcacc
     1501 tcgacgctct ggtacgcgcg ctacatcgac atccggaagg gcatgctcgc gaccttgagc
     1561 ggcaccctcg ccacgatggg ctctgggatt ccctacgcgc tcgctgcgaa ggtcggccac
     1621 tccgacaggc cagtgctcgc gatcgtcgga gacggcgcca tgcagatgaa cggcatcaac
     1681 tcgctcataa ccgtcgcgca acactatcgc aactggcgag accctcggct ggtcatcgtc
     1741 gttttgaaca accagcagct cagctacgtg acctgggagc agcgcgtgat ggagggcgac
     1801 cccaagtttg cgccctctca ggacctcatg gacttcccct tcgcgcgcta cgcggagttg
     1861 ctcggtttgg aagcagcgcg cgtcgcaaac cccgatgagc tcgcggacgt ctaccgccgc
     1921 gctttctctg cgaagcgccc cttcgtggtc gaagcgttta cggatcccaa cgcgccccca
     1981 cttccgccgg agctcaccga agaacaaaag aagatgctcg aacaggcctt taaacaagcc
     2041 gatccagacg tccccgccgc caaacgactc ttcgacaagt tccagagcgt acgctgacgg
     2101 tgtgaacggt ccgccgagac gaggagccaa cctcccgacg aagtgcgttc tccgacggag
     2161 ccttcagctc tgcctgagca
//
LOCUS       DASTKS010001068         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1730969, whole genome shotgun sequence.
ACCESSION   DASTKS010001068 DASTKS010000000
VERSION     DASTKS010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730969"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="queG"
                     /locus_tag="VFQ35_22495"
     CDS             <1..1198
                     /gene="queG"
                     /locus_tag="VFQ35_22495"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9933499.1"
                     /translation="ARLEDATAERLSRAVIDAGIALGFSRVGIADAGPLERGRERLEA
                     FRRRGFAGGLDYLERGDRHDPSALLPGARSAVVALFAHGDPRVVPVEALTSRLRGSVA
                     RYAQGDDYHLLLKTLLFELARRVADLSGRSLRARVCVDSAPLLERELALRAGLGFQGK
                     STLLIAPGVGSYVVIGELLLDVELAPTSGGSAGCGTCRACLDACPTQAFSEEYVLDAR
                     RCISYLTIEREGEIPRELRAPIGNRVFGCDVCQETCPFNASPSRPEASALGRREALAS
                     PDLVALLNLGSAAYRKLVKRTALRRASRTTLQRNAAIALGNSADERALDPLCVALANH
                     ADPIVRLHVAWALGELCASIGPRPQARESLARAALEDPERAVRDECGHALGRGYCSSV
                     AASSAR"
     gene            complement(1162..3348)
                     /locus_tag="VFQ35_22500"
     CDS             complement(1162..3348)
                     /locus_tag="VFQ35_22500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9933500.1"
                     /translation="MPAAISDLSKESRRVFSPRTILSVLVLASVLSAWSMVVGDACAR
                     AGWAYFPLRQHVLGALAMYATVGALLGLLLAALIGLRYVLIGRRQRWPQRARWFASGL
                     FFGLVGGLSSVSTALWTFSGESVQKTALAVWGPILFASAMGGLAAVGAALLARAFESL
                     VRGGREWMVWAALSWLSGLFVAHIDLTQYVSLYSRVHTILELIAALCFAASYALVLLR
                     ALRFERVLRVGGALSLAWLLSTLLFSGVRTWFDDSLRHVWLEEVYVGRMLRRVQVAEA
                     FLADPFHWRGMRWARIERLKARYALAEPKVADVWSAPLAERPERWDALRALRGGQRRY
                     NVVVYYVDTLRADVAADPETMPALERFRKRSLDFRRAYAAGSDTLRSLPVLTGGNYDA
                     LETPENDVLRVAKRASYDDALVIAKSAYEFLGKLRPEFYFDTSLVVEDYPKEQQVWGY
                     GAQRPTAPLLVDRALEFLQKPRKDPFFLWLFNFDQHNWRELDPAYVDGSISKHGIVDD
                     PHKLPYRYRAVAHAIDDEFGRLIAALEKQRRLEDTIVLFVSDHGEALGRDGFWVHSVF
                     LWEPLIRVPLVLHVPGLTPRVVDSKVSLVDVAPTLGRYLDPTLSGAGFHGEDLLGYIL
                     PEPPKRRFPLLLASASKDLLVRVGLVDPEDEFKLVLSFEAALPELYDLRQQDPDGRNF
                     AAQHRARVKQGLALLLGSPIFPHTPDDFDVRNTRAQRALEAATEEQ"
     gene            3662..>4014
                     /locus_tag="VFQ35_22505"
     CDS             3662..>4014
                     /locus_tag="VFQ35_22505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933501.1"
                     /translation="MTRSVPPAPTGSTRPARNGLSAHRLGLYVLFASLTVLFAATVVA
                     FLITRTQTPVWRTPEMPRLPHGLWVSTGLVFAISASFQSALYAVRRNQVAALVRRVGV
                     GIAMAALFLFTQLQN"
BASE COUNT          698 a         1355 c         1314 g          647 t
ORIGIN      
        1 cgcccgcttg gaggatgcga ccgccgagcg gctgtctcga gcggtaatcg acgcgggcat
       61 cgccctcggt ttcagtcgcg tgggcatcgc ggatgccggc ccgctcgaac ggggccggga
      121 gcggctcgaa gcctttcgcc gccgcggctt tgcaggcggg ctcgactacc tcgagcgagg
      181 cgatcgccat gatccgagcg ccttgttgcc tggcgctcgg agcgcagtgg tcgcgctgtt
      241 cgcgcacggc gacccccgag tcgtgccggt agaagccctg acgagtcgcc tgcgcggcag
      301 cgtggcgcgc tacgcccagg gagacgacta ccacttgctc ctcaagacac tgctcttcga
      361 gctcgcacgc cgagtggccg acttgagcgg tcgctcgctg cgcgcccgcg tttgcgtcga
      421 cagcgccccg ctgctcgaac gcgagctggc gttacgcgcc ggtctcggct ttcagggaaa
      481 gtcgacgctc ctcatcgccc cgggggtcgg ctcttacgtg gtcatcggcg agctcttgct
      541 cgacgtcgag ctcgctccaa ccagcggcgg cagcgcaggc tgcggcacgt gccgcgcttg
      601 cctcgacgcc tgcccgaccc aagcgttcag cgaagagtac gtgctcgacg cgcgccggtg
      661 tatctcgtac ctcacgatcg aacgcgaagg tgaaatccct cgcgagctcc gcgcgcccat
      721 cggcaatcgc gtctttggct gcgacgtttg ccaggagact tgcccgttca acgcctcacc
      781 ttcccgcccc gaggcctctg cgctcggtcg ccgtgaagcc ctggcatcgc ccgatctggt
      841 ggcgcttttg aacctcggct ccgccgccta tcgcaagctc gtgaagcgaa cggcgctgcg
      901 ccgcgcgagt cgaacgacct tgcagcgcaa cgccgcgatc gcgctcggca acagcgcgga
      961 cgagcgcgca ctcgaccccc tttgcgtcgc gctcgccaat cacgccgatc cgatcgttcg
     1021 gcttcacgtc gcttgggcac tcggagagct ctgcgcgagc attggtccgc gcccgcaggc
     1081 gcgagagtcg ctcgctcgcg ccgcgctcga agatccggaa cgggcggtgc gcgacgagtg
     1141 cgggcacgcg ctcggccgcg gctattgttc ttcggtcgcg gcttcgagcg cccgctgagc
     1201 gcgggtgttc cgcacgtcga agtcgtctgg agtgtggggg aaaatcggtg agccgagcag
     1261 caacgccaac ccctgcttca cacgagcgcg gtgttgggcg gcaaagttgc gcccgtcggg
     1321 gtcttgctga cgcagatcgt agagctcggg cagcgcggcc tcgaacgaga gaacgagctt
     1381 gaattcgtct tcggggtcga cgagaccaac gcgcaccaga aggtccttcg acgccgacgc
     1441 gagcagcaag ggaaagcgcc gcttgggcgg ttcgggcagg atatagccga gcagatcttc
     1501 gccgtgaaag ccggctccac tgagggtcgg atcgagatag cggccgagcg tgggagccac
     1561 gtccacgaga gagaccttcg aatcgacgac gcgcggggtg agcccgggca cgtgcaggac
     1621 gagcggcacg cgaatcaaag gctcccacaa gaacaccgag tggacccaga acccgtcccg
     1681 accgagtgcc tcgccatggt cggacacgaa caagacgatc gtgtcctcga gccttcgttg
     1741 cttttcgagc gctgcgatca aacggccgaa ctcgtcgtcg atcgcgtgcg cgaccgcgcg
     1801 atagcgatac gggagtttgt gcgggtcgtc gacgatgccg tgcttgctga tgcttccgtc
     1861 gacgtaagcc gggtcgagct ctcgccagtt gtgctggtcg aagttgaaca gccacaaaaa
     1921 gaacgggtct ttgcgaggct tctgcaagaa ttcgagcgcg cggtcgacca agagcggcgc
     1981 ggtcggccgt tgagccccat agccccagac ctgctgctcc ttcgggtaat cttcgacgac
     2041 caggctcgtg tcgaagtaga actctggtcg gagcttgccc aagaactcgt aggcgctctt
     2101 cgcgatgacg agcgcatcgt cgtacgaggc gcgcttggcg acgcgcagca cgtcgttctc
     2161 cggtgtctcg agcgcgtcgt agttgccgcc ggtgagcacc ggcagcgagc gcaacgtgtc
     2221 cgacccggcg gcgtaagcgc gccgaaaatc gagggaacgc tttctgaagc gctcgagcgc
     2281 cggcatcgtc tccggatccg ccgcgacgtc ggctcgcagc gtatcgacgt agtagacgac
     2341 gacgttgtag cggcgctgcc ctcctcggag cgctcgcaac gcgtcccagc gctcggggcg
     2401 ctccgcgagc ggcgcgctcc agacgtcggc caccttcggc tccgcgagcg catagcgagc
     2461 cttcaagcgc tcgattcgcg cccagcgcat gccccgccag tggaaaggat ccgcgaggaa
     2521 tgcttcggcg acctgcacgc gccgcaacat gcgtccgacg tagacctctt cgagccaaac
     2581 gtgccgcagc gaatcatcga accaggtccg aacgcccgag aagagcagcg tcgaaagcaa
     2641 ccaggcgagc gagagcgccc cgccgacgcg caacacccgc tcgaaacgca gtgcgcgcag
     2701 caaaaccagc gcgtacgacg cggcgaagca cagcgccgcg atgagctcga ggatggtatg
     2761 gacgcgcgag tacaacgaaa cgtactgcgt cagatcgatg tgcgcgacga agagcccgga
     2821 gagccacgag agcgccgccc acaccatcca ctcgcggccg ccgcgcacga gcgactcgaa
     2881 cgcgcgcgcg agcagcgccg caccgaccgc ggcgaggcca cccatcgcgg acgcgaacag
     2941 gatcggtccc cagaccgcga gcgcagtctt ttgcactgat tcgccggaga acgtccagag
     3001 cgcggtgctc acgctcgaga gcccgccgac gagcccgaag aagaggccgc tcgcgaacca
     3061 acgcgctcgc tgcggccatc gctgacgccg cccgatgagc acgtatcgaa ggccaatcag
     3121 cgcggcgagc agcaagccga gtagcgcgcc gacggtcgcg tacatcgcca aagccccgag
     3181 cacgtgctgc cggagcggga agtaggccca cccggcgcgt gcgcaggcgt cgcccacgac
     3241 catcgaccag gcgctcagaa ccgaagcgag gaccaggacc gaaaggatgg tgcggggcga
     3301 gaaaacgcgc cgactctctt tggaaaggtc ggaaattgcg gcgggcacgg aggctagcgg
     3361 ggccgggctt tcgctcaaga ttcgtcacat ctcgcgagca ggtgaacctt cgtctcgttc
     3421 gatcgccgaa acctcggcat gaaagggctc tttctttggg ctgcggagga gttcgttccg
     3481 atgcgttggt tcgagttttt ttcggggccc aagaaccccg agaacggcgg gctcgcgccc
     3541 gtttccggag aatctgacga atgcccgggg ttggccgggc agcataattc aggcgtttca
     3601 gggttgtcac gcccctcgac ctggcactac acccccggct gccccatttt cgagcgattc
     3661 catgacgcgc agcgtgcccc ctgccccgac cggctccaca cgccctgcgc gcaatgggct
     3721 ctcggcgcat cgcctcggtt tgtacgtctt gtttgcctcg ctcaccgtgc tcttcgcggc
     3781 aacggtcgtg gcgtttctca tcacgcgcac tcaaaccccg gtctggcgaa ctccggaaat
     3841 gccgcgtctg ccgcacggac tctgggtgag caccggcctc gtcttcgcga ttagcgcttc
     3901 gtttcagagc gcgctttacg ccgttcgccg aaaccaggtc gccgcgctcg tccggcgcgt
     3961 cggcgttggc atcgcaatgg cagcgctatt tcttttcacg cagttacaga attg
//
LOCUS       DASTKS010001069         5791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1956418, whole genome shotgun sequence.
ACCESSION   DASTKS010001069 DASTKS010000000
VERSION     DASTKS010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956418"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..1508
                     /locus_tag="VFQ35_22510"
     CDS             381..1508
                     /locus_tag="VFQ35_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET9933502.1"
                     /translation="MTPATRHRQDSTAPDDIVRVLRDDLTLDPLYDPGLSPAEVMELY
                     KHMVTTRLLDERLVALQRQGRIGFHVGSLGEEAAILGSAYAMRKGDWLFPCYREFGAA
                     LMRGLELQVFVHNMFGNAKDTVRGRQMPDHYTCRRVGWMSISSPVGTQITQAAGFAWG
                     AKIRKQDVATLVYFGDGATSSSDFHSGMNFAGVFKIPTIFLCRNNGWAISVPTQRQTA
                     SRTLAEKGAAYGVPFVRVDGNDLFAVVSVTRRALERGVRGEGPTLIEAITYRMGGHST
                     SDDPGRYRGSEALEPWTSRDPLGRVRAYLEKHNQWDGGAEERLIAEVDQRFREAVGNA
                     ERTPPPPLESMFDDVYERPPWHLQEQRAELLSGPRAPGPHG"
     gene            1669..2643
                     /locus_tag="VFQ35_22515"
     CDS             1669..2643
                     /locus_tag="VFQ35_22515"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9933503.1"
                     /translation="MPQMNMVQAINDALAIAMRADERVVVMGEDVGKVGGVFRVTAGL
                     FDEFGDERVIDTPLSENGIVGSAVGMALYGLVPVVEIQFADFIWPAYDQIVNEMAKLR
                     YRSGGEYPAKVVIRTPVGGGIRGGHYHSQHPEAAFIHVSGLKVVCPSNPHDAKGLLLA
                     SIADPDPIIFFEPKRVYRAAKGEVPQGAYTVPLESAAIVRSGRHVTVLAYGAMLYEAL
                     DAAAKAAQKGVEAEVIDLRTLWPVDIATILESVKRTGRLVVVHEAPKTCGFGAELMSL
                     IVERAFYHLEAPPARVTGFDTPFPYTLEMEYMPLSHRILPTLLDTAKA"
     gene            2674..4023
                     /locus_tag="VFQ35_22520"
     CDS             2674..4023
                     /locus_tag="VFQ35_22520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9933504.1"
                     /translation="MSKFEFKLPDIGEGVTEGEIVNWLVNDGETLAENQDMVEVMTDK
                     ATVTIGAPKAGKVLEKRFKVGDTVPVGEVLVVLDVGGEAQPAAAPAAAPSPAPVAKPE
                     APANGGGTVASAVGDIRENLPGMNVAQQAPATRPEFFADKPAAAPATRKLARELGVDL
                     RTVPPSGSGGRVTREDVERAARPGSPAQEPAAATPAPAAAAAPAPAIEAPRPVSRAED
                     ERVPVRGMRKRIWESMSRSKRSAAHFNYVDEVDVGELIALKDRVKPHADRAGVKLTFL
                     PFIVKAVVAALKRHPRLNAVVDEGSMELVVRKHYDIGIAVATEAGLMVAALREADRLS
                     IVDTAREIERLSGLAKSGKIQKEDLGGTTFTVSSLGKMGGLLAPPIINHPEVGIMGVH
                     AIKRKPVVRGNEIVIGDVMLLSFSFDHRIIDGDVGAVFAQEIISYLETPDRLLVEMS"
     gene            4303..5358
                     /locus_tag="VFQ35_22525"
     CDS             4303..5358
                     /locus_tag="VFQ35_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933505.1"
                     /translation="MGRSPLHLVATLGLAVGVLCARPASAAPQATPTQIREAAEAFDR
                     GREAYKNEDYVTAAEQFERADAQAGSPTALEYAIRSRDRASQLDRAATLAALVAKRYP
                     ENASLEKLASDVQGRARSSLYELSVQCGEPCDLALDDKIVPGAADLTRTLFLQAGHHS
                     LRAGFGNETGGSEEIEATPAGKGQVSFARPAPADVPPPVFDKRPPTAPEKAEPKSSGW
                     SPAVFWVGAGVTGALGIASIWSGVDTLNNPGKDAIREKCGSLGESCPEYQEGLSNQRR
                     TNILLGATAGVGVATILIGVLATDWGSSTPSSPETPKDEIAGHRERRRKRSQAKAVVV
                     PWVSFGSGALVGAEGRF"
     gene            5364..>5791
                     /locus_tag="VFQ35_22530"
     CDS             5364..>5791
                     /locus_tag="VFQ35_22530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9933506.1"
                     /translation="MVAAKLFDPGAGRQRVDRYELLGEIASGGMATVYLARLTGVGGF
                     QRFVAMKRLHPHLANEKEFVEMFLDEARLAAGIHHPNVVPILEVGASPVGYYLVMEYV
                     EGDTLARLLARAASRNSRVPPPIVIRIALDMLAGLHAAHEL"
BASE COUNT         1033 a         1891 c         1907 g          960 t
ORIGIN      
        1 gaggtgtcgg ggggcgtgag tgacgtggag cggcaggtga tggagaaact cgccgagcgg
       61 ctggggctgc cgagtggctc gctcgaacag gctttgttcg aggcgaagac ggtcctcgct
      121 cgctgagggg cgagaatgcg aaagggtcgc tgaattcgag tgcgtagctt actgcgcgcg
      181 gcgctcggag cagccaaact gaagggcggg atcgcgcggt ttgacacgcc gtgcagtcgc
      241 ttacgcacgg gcgaaatttt atccgaatcc gacccgccga tcgttggaaa gcgctccctc
      301 gaagcgagct ttttcgacga cgtttctggt aaccatggca gcataggcgc gcccggctcg
      361 cccgagcatt cagcccaaaa atgacaccgg ctacacgaca ccgccaagac tcgaccgcgc
      421 ccgacgacat cgtgcgcgtg ctgcgtgacg acctaacgct cgatcccctg tacgacccag
      481 ggctctcgcc cgcggaggtg atggagctct acaaacacat ggtgacgacg cgtttgctcg
      541 acgagcgact cgtcgcgctg cagcggcaag gtcgcatcgg ctttcacgtc ggctcgctcg
      601 gcgaggaggc ggcgattctc ggctcggcct acgccatgcg caaaggggat tggctgttcc
      661 cttgttatcg cgagttcggg gcggcgctca tgcggggcct cgagctgcag gtgttcgtcc
      721 acaacatgtt cggcaacgcc aaggacacgg tgcgtggacg gcaaatgccc gaccactaca
      781 cgtgccggcg cgtcggctgg atgtcgatca gctctccggt cggtacgcag atcactcaag
      841 ccgctgggtt tgcgtggggc gcgaagatcc gaaagcaaga cgtcgcgacc ctcgtgtact
      901 tcggtgatgg cgcgacgagc tcgagcgact ttcacagcgg catgaacttc gccggcgtct
      961 tcaagatccc gacgattttt ctctgccgga acaacggttg ggccatcagc gttccgaccc
     1021 agcgtcaaac cgcgagccgc acgctcgccg aaaagggcgc tgcttacggc gtgcccttcg
     1081 tgcgcgtcga cggcaacgac ctctttgccg tcgtctcagt cacgcgacgc gcgctcgagc
     1141 gcggagtccg cggggaaggc ccgactctga tcgaggcgat cacgtatcgc atgggcgggc
     1201 actcgacgag cgacgacccc ggtcgctacc gcggcagcga ggcgctggag ccctggacgt
     1261 cgagggatcc gctcggcagg gtgcgcgcgt acctcgaaaa gcacaatcag tgggacggcg
     1321 gcgcggagga gcgcctgatc gccgaggtcg accaacgttt ccgcgaagcc gtggggaacg
     1381 ccgagcgcac gcccccgcca ccactcgagt cgatgttcga cgacgtgtac gaacgcccgc
     1441 cttggcactt gcaggagcaa cgcgcggagc tcttgtcggg tccgcgcgcg ccgggccccc
     1501 acggctgaag cgcgcggttc gtgttgaacg atcacgttgc cgcttcttcg cggtagcggt
     1561 cgaacgaagc ggtcggcaac ggccctcgcg tttgggagca attcgcttca aaacgattgt
     1621 cagccccgag ggcctacact agcgattggt tacgggacgg aatcagaaat gccccagatg
     1681 aatatggttc aggccatcaa cgacgcgctc gcgattgcca tgcgcgcgga cgagcgtgtc
     1741 gtagtgatgg gggaggacgt cggaaaagtc ggcggcgtgt ttcgcgtcac agcggggcta
     1801 ttcgacgaat ttggcgacga acgcgtcatc gataccccgc tgagtgaaaa cggcatcgtc
     1861 ggcagcgcgg tcggtatggc tctctacggc ctggttccgg tcgtggagat tcagttcgcc
     1921 gatttcatct ggcccgcgta cgaccagatc gtcaacgaaa tggccaagtt gcgctaccgc
     1981 tcgggcggtg agtacccggc caaagtcgtg atccgcacgc cggtgggcgg agggatccgc
     2041 gggggccact atcactcgca gcatcccgag gctgctttca tccatgtttc ggggttgaag
     2101 gtggtttgcc cttcgaaccc ccacgacgcg aagggtttgc tcttggcgtc gatcgccgat
     2161 ccggacccga tcatcttctt cgaaccgaag cgcgtttatc gcgccgcgaa gggtgaggtt
     2221 ccgcaaggcg cgtacaccgt gccgctcgaa agcgccgcga tcgtgcgatc ggggcgccac
     2281 gtcacggtgc tcgcgtacgg agccatgctc tacgaagcgc tggatgcggc cgccaaggcg
     2341 gcccaaaagg gcgtcgaggc cgaggtgatc gatcttcgca cgctctggcc cgtcgacatc
     2401 gcgacgatcc tcgagagcgt caagcgcacc ggccggctcg tcgtcgtgca cgaagccccg
     2461 aaaacctgcg gttttggcgc cgagctcatg agcctgatcg tcgagcgcgc gttttaccat
     2521 ctcgaggcgc ccccggcgcg agtcaccggc ttcgacacgc cgttcccgta tacgctcgag
     2581 atggagtata tgccgctctc gcaccgtatt ttgcccacct tgctcgacac ggccaaagcg
     2641 taggccggcg tccccaatcc ccgagagaga atcatgtcga agttcgagtt caagctcccc
     2701 gacatcggcg aaggcgtcac cgaaggcgag atcgtcaact ggctcgtgaa tgatggcgag
     2761 acgctcgccg agaaccagga catggtcgag gtgatgacgg acaaagcgac cgtcacgatc
     2821 ggcgcgccca aggcgggtaa ggtgctcgag aagcgcttca aggtaggcga caccgtgccc
     2881 gtcggtgagg tgctcgtggt cctcgacgtc ggcggagagg ctcagcccgc cgcggcaccg
     2941 gcggcagcac cgtctcccgc tccggtagcc aagcctgaag cgcccgccaa tggcggcggg
     3001 accgtcgcga gcgccgtcgg cgacatccgc gagaacctgc ccggcatgaa cgtcgcgcag
     3061 caagctccgg cgacgcgccc cgagttcttc gccgacaagc ccgcagccgc tccggccact
     3121 cgcaagcttg cccgcgagct cggcgtcgac cttcgcaccg ttccgccgag cggcagcggc
     3181 ggccgcgtca cgcgcgaaga cgtagagcgc gcggcgcgcc ctggatcgcc cgcgcaggag
     3241 ccggccgccg ctacgccggc tcctgcggct gcagccgctc cggctcccgc gatcgaggct
     3301 ccgcgcccgg tatcccgcgc cgaagacgag cgcgtgccgg tgcgcggaat gcgcaagcgc
     3361 atctgggaga gcatgtcgcg ctcgaagcgc tcggctgctc acttcaatta cgtggacgag
     3421 gtcgacgtcg gcgagctcat cgcgctcaaa gaccgcgtga agccgcacgc cgatcgcgcg
     3481 ggcgtaaagc tcacgttcct gccgttcatc gtgaaggcag tggttgcggc gctaaagcgt
     3541 cacccccgcc tcaacgcggt ggtcgacgag ggcagcatgg agctcgtcgt tcgcaagcac
     3601 tacgacatcg gcatcgccgt cgcgaccgaa gcgggcctga tggtggccgc gctgcgagaa
     3661 gccgatcgtc tcagcatcgt cgacaccgcg cgcgagatcg aacgtctctc cgggctcgcc
     3721 aagagcggca agatccagaa ggaagacctc ggcggcacga cgttcaccgt gagctcgctc
     3781 ggcaagatgg gcgggctcct cgctcctccc atcatcaatc accccgaggt aggcatcatg
     3841 ggcgtgcacg ccatcaagcg aaagccggtg gtgcgcggga acgaaatcgt catcggcgac
     3901 gtgatgctcc tcagcttctc gttcgatcac cgcatcatcg acggggatgt gggagcggtc
     3961 tttgcgcagg aaatcatctc ctacctcgag actcccgacc gcctcctggt cgagatgagc
     4021 tgatcgaata cgcggcaaca cccgcctccg cgcggccgag gcctgctggg tcatggacta
     4081 cctggtctgc ccgccatcga cgaacgacat cggtggcggg ccacaggctc ctacatccgc
     4141 acacacacgt gctgaatcgg cgcgatagct cggccttcag cgcgtcgggc ggacgtgcta
     4201 gcgaacgggg tcgagtgtcc gcgaaccagc gcacgggttt cacggcgggt gctcgcagta
     4261 gcggaacgga gtagtctttt ccctcggatc agcatgctcg taatgggtcg atcccccctt
     4321 cacttggtag ctacgctcgg actcgccgtc ggtgtgctct gcgcgcgccc cgccagcgct
     4381 gcgccccaag cgaccccgac acaaattcgc gaagcagccg aagctttcga tcgcggccgt
     4441 gaggcctaca agaatgagga ctacgtcacc gctgcagagc aattcgagcg cgccgacgca
     4501 caagcgggca gcccaactgc gctcgaatac gcgattcgct cgcgtgatcg cgcgtcgcag
     4561 ctcgatcgcg ctgcgacact ggcggctctc gtcgcgaagc gatatcccga aaacgcctcg
     4621 ctcgagaagc tagcttcgga tgtacaaggg cgagcgcgga gcagcctcta tgagctttcc
     4681 gtgcagtgtg gcgagccgtg tgacctagcg ctagatgaca agattgtccc tggcgcggcg
     4741 gatctcacgc ggacgttgtt cctccaagca gggcaccaca gtctgcgtgc cgggttcgga
     4801 aacgagactg gcggctcgga ggaaatcgag gcgacaccgg ccggcaaagg ccaagtgtcg
     4861 ttcgcgcgcc cggcgcccgc tgacgttccg ccgccggtat tcgacaagcg gcctccgacc
     4921 gcgcccgaaa aggcggagcc caagagttcc ggttggtcgc ccgcggtgtt ttgggttggc
     4981 gccggtgtta cgggcgccct cggcatcgcg tcgatttgga gcggcgtcga tacgctcaac
     5041 aacccaggca aagacgcgat ccgggaaaag tgcggctcgc tcggtgagtc ttgccccgag
     5101 taccaggaag ggctctcgaa ccagcgccgg accaacatct tgctcggcgc aaccgcaggc
     5161 gtcggcgtcg cgaccatcct gatcggagtt ctcgcgaccg attggggttc gagcacgccg
     5221 tcctcacccg aaacgccgaa ggacgaaatt gcggggcatc gcgagcgccg ccgcaaacgc
     5281 tctcaagcga aggccgtcgt cgtgccctgg gtatcgttcg gaagcggcgc actcgtcggc
     5341 gccgaaggaa ggttctgagg cgagtggtcg ctgccaagct cttcgatccc ggagccggcc
     5401 gtcagcgcgt cgaccgctac gagctgctcg gcgagatcgc ctcgggcggc atggcgacgg
     5461 tgtacctggc gcgcctgacg ggcgtcggag gctttcagcg cttcgtcgcg atgaagcggc
     5521 tgcatccgca cctcgccaac gaaaaagagt tcgtcgagat gttcctcgac gaagcgcgtc
     5581 tcgccgcggg gattcatcac ccgaacgtcg tgccgatcct cgaagtgggc gcgagccccg
     5641 tcggctacta cctcgtgatg gagtacgtcg agggcgacac gctcgcgcgg ctcctcgctc
     5701 gcgctgcgag ccgtaactcg cgcgtcccgc cgcccatcgt aatccgcatc gcgctcgaca
     5761 tgctcgccgg tttgcacgcc gcgcacgagc t
//
LOCUS       DASTKS010001070         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_168818, whole genome shotgun sequence.
ACCESSION   DASTKS010001070 DASTKS010000000
VERSION     DASTKS010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168818"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /gene="atpD"
                     /locus_tag="VFQ35_22535"
     CDS             <1..1247
                     /gene="atpD"
                     /locus_tag="VFQ35_22535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9933507.1"
                     /translation="DSTEGLVRGAEVSDQGAPIQMPVGVETLGRILNVVGQPVDERGP
                     VDAKRRMPIHRPAPAFVDQSTKVEIFETGIKVVDLLAPYRKGGKIGLFGGAGVGKTVL
                     IMELINNVAKAHGGVSCFAGVGERTREGNDLYLEMGESKLETGASVLSKTALIYGQMN
                     EPPGARARVALSALTCAEYFRDEEGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGY
                     QPTLANEMGALQERITSTRKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAI
                     SELGIYPAVDPLDSTSTMLSPQIVGDEHYGVARRVQQTLQKYKDLQDIIAILGMDELS
                     DDDRLIVARARKIQKFLSQPFFVAAQFTGVQGKYVPLKETIEGFKEILSGALDDLPEQ
                     AFYMVGNIEEAKQKAQKLKQAA"
     gene            1255..1791
                     /gene="atpC"
                     /locus_tag="VFQ35_22540"
     CDS             1255..1791
                     /gene="atpC"
                     /locus_tag="VFQ35_22540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET9933508.1"
                     /translation="MADTISLEIVTPEGVKLTEQVSELTAPSVAGEFGVLPGHQPMLA
                     ALTTGLVTFSVKGSAEHTAVAVGAGFFEVADDRAVILTDRFIRKADIDPVLVRKELKE
                     VDEALDHFHGDMTVPEYGELVSRQLWAAAQLDLYGDPPPPRVRTISELQFAQHETYAE
                     LADESGSSGETESAPSGH"
     gene            1803..2645
                     /gene="rsmA"
                     /locus_tag="VFQ35_22545"
     CDS             1803..2645
                     /gene="rsmA"
                     /locus_tag="VFQ35_22545"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9933509.1"
                     /translation="MHSSLSPKAQLLALGLTAKRHFGQNFLADAGLCEKVAELCAPSG
                     SSVVELGAGLGALTRPLLSRAAHVTAVERDRDLVPVLRAEFEAEILAERLIVLEADAK
                     AIAPAELLAGRARPHTLCGNLPYQITGPLIELAVRSVESLDRAVFLVQLEVATRLAAS
                     PSGDDYGALSVFTQAAYAVERALVIRRGAFYPQPGVDSAVVVLTPRRDRVQETTVFRD
                     LVHGAFQQRRKKLRNAWASLANGDPAALADVARSANIDLDRRGETLSVEEFARMATLL
                     ESAR"
     gene            2642..>2929
                     /locus_tag="VFQ35_22550"
     CDS             2642..>2929
                     /locus_tag="VFQ35_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933510.1"
                     /translation="MSFRALGFVVALGAIAACRSEARDPDVERVEFGVPFGGDIQDRP
                     KIPLDIEPSELALRVTFREPQRRPRKLSWELERPANSRGADGGLLFSAEIGE"
BASE COUNT          491 a          991 c          972 g          475 t
ORIGIN      
        1 tggattcgac cgagggcctc gtgcgtggcg cggaagtgag cgatcagggc gctcccattc
       61 agatgccggt gggcgtggag acgctcggcc gcatcctcaa cgtcgttggc cagccggtgg
      121 acgagcgcgg cccggtcgac gcgaagcgcc gcatgccgat tcaccgcccg gcacccgcct
      181 tcgtcgacca gagcaccaag gtcgaaatct tcgaaaccgg catcaaggtc gtggacctcc
      241 tcgctcccta ccgtaagggc ggcaagattg gtctgttcgg cggcgctggc gtcggcaaga
      301 ccgtgctcat catggagctc atcaacaacg tcgcgaaggc gcacggtggt gtgagctgct
      361 tcgccggcgt cggcgagcgc acccgcgaag gcaacgatct ctacctcgaa atgggcgagt
      421 cgaagctcga gaccggcgcg agcgtgctct cgaagacggc cctcatctac ggtcagatga
      481 acgagccgcc gggcgcccgc gcgcgcgtcg ccttgagcgc gctcacctgt gccgagtact
      541 tccgcgacga ggaagggcag gacgtgctcc tgttcgtcga caacatcttc cgcttcacgc
      601 aagcgggcag cgaggtgtcg gcgctcctcg gccgtatgcc gagcgcggtg ggttatcagc
      661 ccaccctcgc gaacgagatg ggtgctctcc aggagcgcat cacctcgacg cgcaagggca
      721 gcatcacgag cgtgcaggcc atctacgtgc ccgccgacga tctcactgac cccgcgcccg
      781 ccacgacctt cgcgcatctc gacgcgacga cggtgctctc ccgcgcgatc tccgagcttg
      841 gcatctaccc ggcggtggat ccgctcgatt cgacgagcac gatgctgagc ccgcaaatcg
      901 tgggcgacga gcactacggt gtcgcccgcc gcgtccagca gaccttgcaa aagtacaagg
      961 atctgcagga catcatcgcg attctcggca tggacgagct cagcgacgac gatcgcttga
     1021 tcgtcgcgcg cgctcgcaag atccagaagt tcctatcgca gccgttcttc gtcgcggcgc
     1081 agttcaccgg cgttcagggc aagtacgtac cgctcaagga gacgatcgag ggtttcaagg
     1141 agatcctctc gggcgcgctc gacgacctgc cggagcaagc cttctacatg gtcggcaaca
     1201 tcgaagaggc caagcagaag gcccaaaagc tgaagcaagc ggcgtgagcg cacgatggcc
     1261 gacaccatct ctctcgagat cgtgacgccg gaaggcgtga agctcaccga gcaggtgagt
     1321 gagctcacgg cgccgagcgt cgctggagaa ttcggtgtgc tgccggggca tcaaccgatg
     1381 ctcgcggcgc tcacgaccgg gctcgtcacc ttctcggtga agggcagcgc cgagcacacg
     1441 gcggtggcag tgggcgccgg cttcttcgaa gttgccgacg accgcgcggt catcttgacc
     1501 gatcgcttca tccgcaaggc cgacatcgac ccggtgctcg tgcgcaagga gctgaaggaa
     1561 gtggacgagg cgctcgacca cttccacggc gacatgacgg tgccggagta cggcgagctc
     1621 gtctcgcgcc agctgtgggc ggccgctcag ctcgaccttt acggcgatcc gcctccgcct
     1681 cgcgttcgca ccatcagcga gcttcagttc gcccagcacg aaacctacgc cgaactcgcc
     1741 gacgagtcgg gttcgagcgg cgaaaccgag tcggccccga gcgggcactg acggcggccg
     1801 cggtgcattc gtcgctgagc cccaaagcgc agctgctcgc gctggggctc accgcgaagc
     1861 gccatttcgg ccagaatttc ctggccgacg ctggcctctg cgaaaaagtc gcggagctct
     1921 gcgcgccctc tggctcgagc gtcgtcgagc tcggcgcggg gcttggcgcg ctcacgaggc
     1981 cgctactttc gcgcgcggcg cacgtcacgg cggtggagcg cgatcgcgat ttggtcccgg
     2041 tgctccgggc agagttcgaa gcagagattc tggccgagcg cctcatcgtg ttggaggcag
     2101 acgccaaggc gatcgctccg gcggagctcc tcgccgggcg cgcgcggccc cacacgctct
     2161 gcggtaacct cccgtatcag atcacggggc cgctcatcga gctcgcggtg cgctcggtcg
     2221 aaagcctcga tcgcgccgtg ttcctggtgc agctcgaagt ggcgacgcgc ctcgccgcgt
     2281 caccgtcggg tgacgattac ggtgctctca gcgtcttcac tcaggcggcc tacgcggtcg
     2341 agcgggcgct cgtcatccgc cgcggcgcat tctaccccca gcccggcgtc gattcggcgg
     2401 tggtcgtgct cacgccgagg cgcgatcgcg tccaagaaac cacggtgttt cgcgatctcg
     2461 tgcacggcgc ctttcagcag cgacgcaaga agctacgcaa cgcctgggcg tcgctcgcga
     2521 acggcgatcc agccgcgctc gccgacgtcg cccggtcggc caacatcgat ctagatcggc
     2581 gcggcgagac actgtccgta gaggagttcg cgcggatggc gacgttgctg gagagcgcgc
     2641 ggtgagcttt cgagcgctcg gcttcgtcgt cgcgctcggc gcgatcgcgg cctgccgcag
     2701 cgaagcgcgg gatcccgacg tggaacgcgt cgaattcggt gttcccttcg gcggcgacat
     2761 ccaagatcgg ccaaagatcc cgctcgacat cgagccctcc gaactcgctc tgcgcgtcac
     2821 tttccgcgag ccgcagcgcc gtccgcgtaa gctcagctgg gagctcgagc gcccggcgaa
     2881 ctcgcgcggt gcggacggtg ggctcttgtt ctcggccgag atcggcgag
//
LOCUS       DASTKS010001071         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1732079, whole genome shotgun sequence.
ACCESSION   DASTKS010001071 DASTKS010000000
VERSION     DASTKS010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732079"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="nth"
                     /locus_tag="VFQ35_22555"
     CDS             <1..867
                     /gene="nth"
                     /locus_tag="VFQ35_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9933511.1"
                     /translation="KAKARPLQTATPTPAARAAMAAKKADAKARASARTRPASKPTKG
                     AKPAKSAPKANPKAAAGKPPSLVSPKIALREFAALREQHPDAHCELDHENPFQLLVAT
                     VLSAQSTDVMVNKITPEVFERWPTPEALAKADPAAVEHALSRIGMFRQKTRNIIGLSK
                     LLVSEHGGKVPQSLAELVKLPGVGRKTANVVLGVAFGKPEGVVVDTHVQRLSQRLGFT
                     KQTEPLEIEQDLMKIFPESDWDPLSHVLIFHGRRVCSARKPACSACSAQKDCPSAFRA
                     ENVGRKPPRLRA"
     gene            1001..1840
                     /locus_tag="VFQ35_22560"
     CDS             1001..1840
                     /locus_tag="VFQ35_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933512.1"
                     /translation="MRKIELLRVASSLAGGPALLTLSALLLVAPAARASERHFGFTYE
                     SEVLAPGEAELEPWTTVRAGRRHFYSAFDQRLELELGLAPNLQTSLYWNFGASAETLR
                     DPVTQERARKEDFYLTSISNEWKYRLSDPLADAIGSALYFEASFGPREIELEGKLIVD
                     KQVGNWLFAGNLAGEQEWELALGEDESEQHYWVNLAAGYFVTPALVAGLEAMSLTTME
                     EGEVESSAIYGGPSLAYATERYWLVASGAPQLFAPKGESGRHLDLHGGERVWARLLLG
                     FRI"
     gene            1837..2106
                     /locus_tag="VFQ35_22565"
     CDS             1837..2106
                     /locus_tag="VFQ35_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9933513.1"
                     /translation="MKAFFRGSVRARLLAGLGLTFVACGAGLPHPTNQHLNIAKQSEP
                     NVSLADLERGRSLYVQKCGSCHALRAPQSLAPEQWRPEIEKMRTQ"
     gene            complement(2231..>2806)
                     /locus_tag="VFQ35_22570"
     CDS             complement(2231..>2806)
                     /locus_tag="VFQ35_22570"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9933514.1"
                     /translation="GPLFAQDTAPVPERVERFLSARDERPLVYVALTSTKTWVVQGAI
                     AAARRAGARVLAVSTVHELGLTEGDDLLIEPYLPSLRVMPRVALAITAGGQGSVQTAM
                     VSGTPLLGVPLQPEQDLNLHLIERQGAGRLLPLAEVRAERMAPIVRSMLATASYRVNA
                     ERLQGLLRNVDGAGRAAELMMRLAEGGIEAV"
BASE COUNT          530 a          926 c          924 g          426 t
ORIGIN      
        1 aaggcgaaag cgcgcccact gcagacggcg acaccgacgc cggcggcgag ggcagcgatg
       61 gcggcgaaga aagctgacgc gaaggcacgg gcttcggccc gaacccggcc cgcatcaaag
      121 ccgaccaagg gggcgaagcc cgcgaagagc gcccccaagg ccaaccccaa agcagccgcg
      181 ggcaagccgc cctcgctcgt gagcccaaag atcgccttgc gcgagttcgc ggcgcttcgg
      241 gagcagcacc ccgacgcgca ctgcgagctc gatcacgaaa atccgttcca gctcttggtc
      301 gcgacggtgc tctcggcgca gagcaccgac gtgatggtga acaagatcac gcccgaagtg
      361 ttcgagcgct ggccaacgcc ggaagcgctc gccaaagcgg atccggctgc ggtcgagcac
      421 gcgctgagcc gcatcggtat gtttcgccag aagacgcgca acatcatcgg gctctccaag
      481 ctgctcgtct ccgagcacgg cggcaaagtg ccgcagtccc tcgcggagct cgtgaagctc
      541 cccggggtcg gccgcaaaac cgcgaatgtc gtgctcggcg tcgcgttcgg aaagcccgaa
      601 ggcgtggtcg tcgacacgca cgtgcagcgc ttgagccagc gcctgggctt cacgaagcaa
      661 accgagccgc tcgaaatcga acaagatcta atgaagatct ttcccgaatc ggactgggat
      721 ccgctctctc acgtgctgat cttccacggc cgccgcgtgt gcagcgcacg aaagccggcg
      781 tgctcggcct gctcggctca aaaggactgc ccgtccgcgt ttcgagccga aaacgtcggc
      841 cgcaagccgc ctcggctccg cgcctgagcc gccaggcgtc cacgcctgag ccgccgcgcg
      901 cgcaggcgtg agctggacgc gcgcgcagtg acggagcgca gcgccagcgg caagcgatat
      961 tgacagtcat tttcaataag cgtaacggtt ccagagttcc gtgagaaaaa tcgagctttt
     1021 gcgagttgct tcgagtcttg ccggcggccc agcgctgttg acgctctcgg cgttgctcct
     1081 cgtggcgcct gccgcgcgag cgagcgaacg ccacttcggg ttcacctacg agtcggaggt
     1141 cctcgcgccg ggcgaagccg agctcgaacc gtggaccacc gtgcgcgcgg ggcgtcgcca
     1201 cttctacagc gcgttcgacc aacgcctcga actcgagcta ggcctcgctc cgaaccttca
     1261 gacgtcgctg tattggaact tcggcgcgag cgcggaaacg ctgcgcgatc cggtcacgca
     1321 agagcgcgct cgaaaagaag acttttacct cacgtcgatc tcgaacgagt ggaagtaccg
     1381 cctgagcgat ccgctcgccg acgccatcgg cagcgcgctg tatttcgagg cgagcttcgg
     1441 gccgagggag atcgagctcg agggcaagct catcgtcgac aagcaggtcg gcaactggct
     1501 cttcgcaggg aacctcgccg gtgagcaaga gtgggagctt gcgctcggag aggacgagag
     1561 tgagcagcac tattgggtga accttgccgc gggctacttc gtgacgcccg cgctcgtcgc
     1621 tgggctcgag gcgatgagcc tgaccaccat ggaggaaggc gaggtcgaat cctctgccat
     1681 ctacggtgga ccaagcctcg cctacgcgac cgaacggtac tggctcgtcg cgagcggcgc
     1741 gccccagttg ttcgcgccta agggcgagag cgggcggcac ttggatcttc acgggggcga
     1801 gcgcgtgtgg gcacgtctgc tgctcgggtt ccgcatatga aggcgttttt tcgcggaagc
     1861 gtgcgcgcgc ggctgctcgc tgggctcggt ctgacgttcg tcgcgtgcgg cgctggcctc
     1921 ccccacccga ccaaccaaca cctgaacatc gccaagcaga gcgagcccaa cgtgtcgctc
     1981 gcggatctcg agcgagggcg ctcactctac gtgcagaaat gcgggagctg tcacgcgctg
     2041 cgagcgccgc aaagcctcgc gcccgaacag tggcgacccg agatcgagaa gatgcggacg
     2101 caataaggcg tgcgactgaa gagcgacgag accgacgaca tcaatcggta tctcgcggtc
     2161 gtgagcgcga gcactcggta gccccgagcc caagcgaacc acgcgtcgcg gcgaagcgtg
     2221 tctctaccga tcaaacggcc tcgatgccgc cttcggcgag tcgcatcatc agctcggcgg
     2281 cgcgtccggc accgtcgacg ttgcgcaaca acccttgaag ccgctctgcg ttgactcgat
     2341 agctcgccgt cgcaagcatc gaccgaacga tcggtgccat gcgctctgct cggacttctg
     2401 ccaacggcaa caaacggccc gcgccttgcc gctcgatgag atgaagattc aggtcttgtt
     2461 cgggctggag cgggacgcca agcagcgggg tgccggatac catcgcggtt tgcacgctgc
     2521 cttgcccgcc tgcggtaatg gcgagcgcga cgcgcggcat gacccgaagg ctcggcaaat
     2581 agggctcgat caggaggtcg tcgccttcgg tcaacccgag ctcgtgcacg gtcgacaccg
     2641 ctaaaacgcg cgcgccagcg cggcgggcgg cagcgatggc gccctgaacc acccaagtct
     2701 tcgtggacgt cagcgctacg tacacgagcg ggcgctcgtc gcgagcggac aagaagcgct
     2761 ccacgcgttc cggaaccgga gcggtgtctt gcgcgaagag cgggcc
//
LOCUS       DASTKS010001072         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_838263, whole genome shotgun sequence.
ACCESSION   DASTKS010001072 DASTKS010000000
VERSION     DASTKS010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838263"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFQ35_22575"
     CDS             <1..343
                     /locus_tag="VFQ35_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9933515.1"
                     /translation="KCVLARKSRWARLRLRALHRLLFVSLLLLPLASRPKARAFDKIG
                     TLDLVRGYARMLDAVFAQTPELGLAAPRMVLWALGVARDDLGTNDVLAASIEAAERAA
                     DRSAVSVTALR"
     gene            complement(345..1289)
                     /locus_tag="VFQ35_22580"
     CDS             complement(345..1289)
                     /locus_tag="VFQ35_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933516.1"
                     /translation="MRLPALVLTIVAVLAARRAGADPPRADVELGDDARLARVVNLYE
                     AGKYSECATELGVMLAPEAPRKLRDREVLENARIYHAACLIGSGDVAAADEPLRAAIR
                     ANIQMKAPDSLVFPPPVIERFLRVRQSLYEEIKKAEEQRVQQARRAAQEQAARARAEK
                     ERVEALERLATQEVTVVKNRRWVALVPFGVGQFQNGDEGLGWVFLTSETVLAATAFTS
                     IAMQTGLQLEAQRLHNPANNAVLSTWHTLVVVSSYAFLGVAATGIAQAQLGFVPETRV
                     VRERPLPLELRRKTSSISLEPNVSVVPGSAFVGLSGRF"
     gene            complement(1316..3163)
                     /locus_tag="VFQ35_22585"
     CDS             complement(1316..3163)
                     /locus_tag="VFQ35_22585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933517.1"
                     /translation="MGATRELSPKLEKYELLEEIGHGGMATVYRARDKRLGREVAVKI
                     IHRHLRENQEVAQRFQSEARAVAKLRHPNIVEVFDVSDASEDERYLVVELVRGTTLRK
                     LLSQHGAFPPEVAAAVGIALASALDHAHRLGVIHRDVKPENVLVGDAKPTGSTEPEPV
                     PIKLTDFGIAKLLDAQGVTSTGQVLGSPAHMAPEQIEGGEVSARSDVFGLGVLLYECM
                     VGRLPFDGKNPAQVLRRVLDGAFTPADRAEPRVGAAFSAILSKALSHVAADRYASTAD
                     LATALREELTALGFDSSASELEAFLSDPARYKKEHEERIVARLTARAENLQKVRNVPS
                     AAACFNRALAYRPQDPRLLAAVSTLARGARARRFVERGALALLSVLLVAAGALALARR
                     SPARLDAPRSDVSSAVPSSAPSAVTGPKASAGVQPAASASAAVSASPTTRRNVVPRPT
                     PRPSAEPSDAAVLGEQLARVQIVVNGPQSATVRIDGRETPWFGKIQELTPGEHVFEFI
                     PPDEQCCEPGQTLRVKVRPASGPADVQTVQGRIEFRPATFDIRGTAGSVVTCGALGEF
                     PVPSQQQVPLRDGPRRVRCQFIPPPGSVDPPKEFDVTLRPGRLSTNLGQ"
     gene            complement(3191..>3478)
                     /locus_tag="VFQ35_22590"
     CDS             complement(3191..>3478)
                     /locus_tag="VFQ35_22590"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HET9933518.1"
                     /translation="ETGVTARLVSKARAMVPILAFSSEERTLRQLALLWGVGPRFLAT
                     PKANFEEQVHETTQYLLQQGLVKSGERYVMVYGARVGVRGATNAVRVEQLP"
BASE COUNT          533 a         1185 c         1188 g          572 t
ORIGIN      
        1 gaagtgcgtg ttggcgcgca agtcgcgctg ggctcgcctt cgcttgcgcg cgctgcaccg
       61 gctgctcttc gtgagcctgt tgctgctccc gctcgcgagc cgccccaaag cgcgagcatt
      121 cgacaagatc gggacgctcg atctcgtccg cggttacgcg cgcatgctcg acgccgtgtt
      181 cgcgcaaacg cccgagctcg ggctcgctgc gccccggatg gtcttgtggg cgctcggcgt
      241 cgcccgcgac gatctcggaa ccaacgacgt gctcgccgcg agcatcgagg cggcggagcg
      301 agcggcggat cgcagcgccg tttccgtcac tgccctgcgt tgattcagaa ccgcccggag
      361 aggccgacga acgccgagcc cggcacgacc gagacgttcg gctcgagcga gatggagctc
      421 gtcttgcggc gcaactcgag cggcagcggt cgctcacgaa cgacgcgcgt ctccggtacg
      481 aagccgagct gagcctgagc gatgccagtc gccgcgacgc cgaggaaagc gtagctgctg
      541 acgaccacga gcgtgtgcca cgtcgagagc accgcgttgt tcgcgggatt gtgcaggcgt
      601 tgggcttcga gctgcagccc ggtttgcatc gcgatcgagg tgaacgcggt cgccgcgagc
      661 acggtttcgc tcgttagaaa gacccagccg agaccctcgt cgccgttctg aaattggccg
      721 actccgaacg gaacgagcgc gacccagcgc cggttcttga cgacggtcac ctcttgcgtc
      781 gcgaggcgct cgagcgcctc cacgcgctcc ttctcggcgc gagctcgcgc tgcctgctct
      841 tgcgccgcgc gtcgcgcctg ctgcacccgt tgctcctccg ccttcttgat ctcctcgtac
      901 agtgactggc gcacgcgcag aaagcgctcg atcaccggcg gcgggaacac caagctgtcg
      961 ggcgccttca tctggatgtt ggcgcggatg gcggcgcgca gcggctcgtc tgcggccgcg
     1021 acgtcaccgc tgccaatgag acacgccgcg tgatagatgc gcgcgttttc gagtacttcg
     1081 cggtcgcgaa gcttgcgcgg ggcttcggga gcgagcatca cgccgagctc ggtcgcgcac
     1141 tcggaatact tccccgcctc gtagaggttt acgaccctcg cgaggcgcgc gtcgtccccg
     1201 agctcgacgt ctgcgcgggg cgggtcggcc cccgcgcgtc gcgcggcgag gaccgccact
     1261 atcgtcagta ctagagccgg aagccgcatc gagccgcctc gagctcagaa cccgttcact
     1321 gcccgaggtt cgtagagagt cgtccgggcc tcagcgtcac gtcaaactct ttcggcgggt
     1381 cgacgcttcc gggcggtgga atgaactgac atcggacgcg ccgcggaccg tcgcggagcg
     1441 gcacctgctg ctggctcggc accgggaact ctcccaaggc gccacacgtc acgacgcttc
     1501 ccgccgttcc tcggatgtcg aaagtggctg gtcgaaactc gattcggcct tgcaccgttt
     1561 ggacgtcagc cggaccgctg gctgggcgta ctttcacccg cagcgtctga ccgggttcgc
     1621 agcattgctc gtccggcggg atgaattcga aaacgtgctc gcccggcgta agctcctgga
     1681 tctttccaaa ccagggcgtc tccctgccgt cgatgcgcac cgtggcgctc tgcggaccgt
     1741 tcacgacgat ctgcacgcgc gcgagctgct cgccgagtac cgctgcgtcc gatggctctg
     1801 ccgaaggtcg cggggtagga cgtgggacga cgttgcgccg cgtcgtcggg gaagcagaaa
     1861 cagccgcgct ggcagacgct gctggttgca cgcctgcgct cgccttgggc ccggtgacag
     1921 cgctcggagc ggacgaggga accgcgctgc tcacgtccga gcgcggcgca tcgaggcgcg
     1981 ccggagaacg ccgcgcgagg gcgagcgccc cggcggcaac gagcaacacc gagagcagcg
     2041 ccagcgcccc gcgctcgacg aagcgtcgcg cacgggcgcc gcgggcaagc gtcgagacgg
     2101 cagcgagcaa ccgcggatcc tgcggacggt aagcgagcgc ccgattgaag cacgccgcgg
     2161 cgctcggcac gttccgcact ttttgaagat tctcggcccg agccgtgagg cgcgcgacga
     2221 tgcgctcctc gtgctctttt ttgtaccgcg ccgggtcgga gaggaatgct tcgagctctg
     2281 acgcggatga gtcgaagccg agcgcggtga gctcttcgcg cagtgccgtt gcgaggtccg
     2341 ccgtgctcgc gtagcggtcg gcggcgacgt gggaaagcgc cttagatagg atggcggaga
     2401 aagctgcgcc aacgcgcggc tccgcccgat ccgccggggt gaaggcgccg tcgagaacgc
     2461 gccgcaagac ctgcgccggg ttcttcccgt cgaacggcaa acgcccgacc atacactcgt
     2521 agaggagtac gccgaggccg aagacgtccg agcgagccga gacctcgccg ccttcgatct
     2581 gttccggcgc catgtgcgcg ggcgaaccga gcacctgccc cgtcgaggtc acgccctgcg
     2641 cgtcgagcag cttcgcgatg ccgaagtcgg tgagcttgat gggcaccggc tccggctctg
     2701 tagaaccggt cggcttcgcg tcgccgacca gaacgttctc gggctttacg tcgcggtgaa
     2761 tgaccccgag ccgatgcgcg tggtcgagcg cgctcgcgag cgcgatgccg accgcagcgg
     2821 caacctcggg tgggaaagcg ccgtgctgcg agaggagctt gcgcagtgtc gtgccgcgca
     2881 cgagctccac gacgaggtag cgctcgtctt cgctcgcgtc ggagacgtcg aatacttcta
     2941 cgatgttcgg gtggcggagc ttcgcgaccg cgcgcgcctc gctctggaag cgctgagcga
     3001 cttcttggtt ctcacgcagg tgacggtgga tgatcttgac ggcgacttcg cgcccgagac
     3061 gtttgtcgcg agcgcggtac accgtggcca tgccaccgtg gccaatttcc tcgagtagct
     3121 cgtatttctc gagcttcggg ctgagctccc gcgttgctcc cagagtgccc ccgtctcagt
     3181 tacgccgccg tcacggcagc tgctccacgc ggacggcgtt cgtcgcgccg cgcacaccaa
     3241 cgcgcgcgcc gtacaccatg acgtagcgtt cgccggactt aacgaggccc tgctgcaaga
     3301 ggtactgcgt cgtctcgtgc acctgctcct cgaagttcgc tttcggcgtc gcgaggaagc
     3361 gagggccaac gccccagagc agcgcgagct ggcgcagcgt gcgctcctcg gacgagaacg
     3421 cgaggatcgg caccatggcg cgcgccttcg aaacgagccg cgcggtgacg cccgtctc
//
LOCUS       DASTKS010001073         6918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_838309, whole genome shotgun sequence.
ACCESSION   DASTKS010001073 DASTKS010000000
VERSION     DASTKS010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838309"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFQ35_22595"
     CDS             <1..424
                     /locus_tag="VFQ35_22595"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9933519.1"
                     /translation="LSYLHGFGNIVPGLEKALEGKGVGDALKVVVAPDDAYGERDEKL
                     VQAVPRDEFPDGEIAVGMRFRADSPSGSKVLTVVALNDSEVTVDANHPLAGCTLSFDV
                     KVRGIRDATQEELDHGHVHDGHHHHHDDDDDDHGHHHH"
     gene            complement(451..903)
                     /locus_tag="VFQ35_22600"
     CDS             complement(451..903)
                     /locus_tag="VFQ35_22600"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9933520.1"
                     /translation="MKTKYALKALCRLALSQTGEPVLIADIAEAENIPRKFLELILGE
                     LKQHGMLQSRKGRGGGYLLAKNPDTITVASVLRVLDGPIAPVPCLSRTAYQRCAECAD
                     EATCGVRLILKDAHAASLQVLENTTLADMVERVNGAARGPSPVLKYSI"
     gene            complement(1006..1644)
                     /locus_tag="VFQ35_22605"
     CDS             complement(1006..1644)
                     /locus_tag="VFQ35_22605"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9933521.1"
                     /translation="MSLHIGSIAPDFEQDSTHGRIRFHEWLGSSWGILFSHPKDFTPV
                     CTTELGRAANLKKEFEARGIKMIAVSVDDVDSHKRWVKDIDETQSCSLDYPILADSDR
                     KVATLYDMIHPEANDTLTVRSTFIIDNNKKIRTIITYPASTGRNFQEILRVIDSLQLT
                     DNYKVATPVDWKQGEDVVIVPALQDPAELQQRFPKGYRVVKPYLRLTPQPNR"
     gene            1802..2239
                     /locus_tag="VFQ35_22610"
     CDS             1802..2239
                     /locus_tag="VFQ35_22610"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9933522.1"
                     /translation="MLLDEIKARMFKAMKAGDGLEKEILRVAIGEITTDSARPGRKGN
                     DDEAQAILRKLVKSNEETIASLEEGEKKSSLLRENQILSDFLPKSLSEDEVVAALAPV
                     IEQVRAAANDGQATGVAMKHLKASGALVDGKVVTKAVQRVRAG"
     gene            2339..2488
                     /locus_tag="VFQ35_22615"
     CDS             2339..2488
                     /locus_tag="VFQ35_22615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933523.1"
                     /translation="MEDGEGRLVERLEAQSQVTSVLTRATTVHDARDHDLRVYVYSVA
                     DAERK"
     gene            2835..3725
                     /locus_tag="VFQ35_22620"
     CDS             2835..3725
                     /locus_tag="VFQ35_22620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9933524.1"
                     /translation="MSRYPRATPSAPIPKASVPPADRRLSAVLVAPVVVVAPDVEREL
                     ISGSLRIGRHPSSDIVLEDALVSRTHARLVVQEDGSLVIEDLHSANGVYVNGERLGRH
                     SHRLCEGDRLLIGTCELSVFSCRAAPSLAEPPSVRSAIARVLDTDAEGPPTTERTDGL
                     QMVARLAERFHRAGNSMEAIRVLSGHLNKVLLGASAGLAVPSELLDEASRHAVELYYW
                     TYNGAWIDYVIELHLATQALPSERTVATLEAAIASSSGTPFDAGLLKYFLESQASTKA
                     PRTLAEEARLSRLERLRSQR"
     gene            complement(3785..5233)
                     /locus_tag="VFQ35_22625"
     CDS             complement(3785..5233)
                     /locus_tag="VFQ35_22625"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9933525.1"
                     /translation="MIKNRNRRAFLRAVGAGFATLPFFRLLENSYAQAAGDTLPLKFV
                     GIYHPHGVAAELWAMKDSDTETKFDFSYADCSLKPFDDAATYGKSFKDKIIVVEGIDH
                     LSNANGHDSAGTILTGSRIDGNKKALNSSLDQFLAVEKGLGSQTRIASIALAVGTSTL
                     SSGECLSFGPGGAPLAKIIDPTETFKQLFAGFAAGNDSAAQAALARENKNGQSVIDFV
                     RGDINRLRGRLAAPEQQKLDQHLTALREIEKQFESPTMSGCAVPSKPDATKFPKLQRY
                     NGGEPYFDAITDIQLDLLAQAFACDITRFATVFLNDLSYDGNPLGLPADNHGSVAHTY
                     AASPIGNNGAPVGAGTPSTWTPLAKFNKYSYGKIARFMQKLDQLGVLDSTLIYASSDM
                     GNPALHSTRNVPTLLAGGVNGKFKMGRRLKMQADCPASNAWCGEADAVFTPVTNNKIL
                     VSIAQAFGVDVNTFGTQPDSKLTTGALSQLTG"
     gene            complement(5230..>6918)
                     /locus_tag="VFQ35_22630"
     CDS             complement(5230..>6918)
                     /locus_tag="VFQ35_22630"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9933526.1"
                     /translation="ETGGSAQPNAGTGSGVSQGGSGNSSNGGGANPSGGKSNPGGGSG
                     STAGSPPMTSGGSTPLPSGVEPSEMIPQRIRRLANAEFDASLKNLTGTDKAPAQSFAP
                     DARQSGFTLNDAQRVDNVTAKQIYAAASDAASSFDVTKNAPCQDTSKAEACAQSFIAS
                     YGAKAYRRPLTTEESAGLLAVYKTGADGATYADGIRLVVRALVNSAGFLYLTEIGNAG
                     VAAPGTAAALTPYELASSLSFLVTNAPPSEQLVADAVAGKLDTPEGRGKAFDDLVRTP
                     AARDRIVRVVREWVGVDRISETDKDSNIYPNFAGLKAAMVTESQQFVENVLTGTGTVG
                     ELLGANWTMVTDTKLAQLYNANGSGRVTLPTRRGILNQAAWLSVFAHAHESSPVLRGV
                     DIARRFACLNIASPTTLNIAVVPPPPDPSKTTRARFSTHSTDSICAGCHKNIDQFGFA
                     FEEYDGMGTFRTKEGSNDVNSKVTINSGTDFDGDYADGNALAVAMSKSPQVTACFARH
                     MFRGSAARSDDTVQASEDAFIRYWQSLPMDQQGNILQTLATFVKSPLFTHRRAP"
BASE COUNT         1184 a         2214 c         2274 g         1246 t
ORIGIN      
        1 gctcagctat ctgcacggat tcggcaacat cgtccccggg ctcgagaaag cgctcgaggg
       61 caagggcgtg ggcgacgcct tgaaggtcgt cgtagcgccc gacgacgcct acggtgagcg
      121 cgacgaaaag ctcgttcaag ccgtcccgcg cgatgagttc cccgacggag agatcgcggt
      181 cggcatgcgc ttccgcgccg actccccgag cggcagcaag gtgctcacgg tcgtcgcgct
      241 gaacgacagc gaagtgaccg tggacgcgaa ccacccactc gccggttgca cgttgtcatt
      301 cgacgtcaag gtgcgcggta ttcgagacgc aacccaagaa gagctggatc acggccacgt
      361 tcacgatggt catcatcatc accacgacga tgacgacgac gatcacggcc accaccacca
      421 ctgacgccgc gagcgctcac gccctcctgg ctaaatcgaa tacttgagca ccgggctcgg
      481 gccgcgagca gcgccgttca cccgctcgac catgtcggcg agggtcgtgt tctcgagcac
      541 ctggagcgac gcggcgtgcg catctttcaa gatcaagcgc acgccgcagg tcgcctcgtc
      601 tgcgcactcg gcgcagcgct ggtacgccgt gcgactcaga caggggacgg gcgcgatggg
      661 accgtcgagc acccggagca ccgaggcgac ggtgatcgtg tcggggttct tggcgagcag
      721 atagcctccc ccgcgcccct tccgactttg gagcattccg tgctgcttga gctcccccaa
      781 gatgagctcg aggaacttgc gaggtatgtt ttctgcctcg gcgatgtcag cgatgagaac
      841 gggttccccc gtctgggaaa gcgcgaggcg gcacagcgcc ttcaacgcgt acttggtctt
      901 catcgtgatc atgctggcct gagcgagccg gcgctcgcgg acgggagctt gccagcggat
      961 gggcccgcgg caagcccctt tcgcgcggcg cggatttagc ggccttcagc gattcggctg
     1021 cggggtaagg cgcaggtagg gcttcacgac gcgatagccc ttggggaagc gctgctgaag
     1081 ctccgccggg tcctgcaggg ccgggacgat cacgacgtct tcgccctgct tccaatccac
     1141 cggggttgcg accttgtagt tgtcggtgag ctgcagcgag tcgatgacgc gcaagatctc
     1201 ttggaagttg cgccccgtgc tcgccgggta cgtgatgatg gtgcgaatct tcttgttgtt
     1261 gtcgatgatg aaggtcgagc gcaccgtcag cgtgtcgttc gcttccgggt ggatcatgtc
     1321 gtacagggtc gcgaccttgc gatccgagtc tgcgagaatc gggtaatcga ggctgcagct
     1381 ctgcgtctcg tcgatgtcct tgacccatcg cttgtggcta tccacgtcgt cgacgctcac
     1441 ggcgatcatc ttgatgccgc gggcctcgaa ctccttcttc aggttcgcgg cgcgaccgag
     1501 ctccgtcgtg cacaccggcg tgaagtcttt cgggtgggag aacagaattc cccaactcga
     1561 accgagccac tcgtgaaagc gaatgcggcc gtgcgtcgaa tcctgctcga aatcgggcgc
     1621 gatgctgccg atgtgtagcg acatgaagaa cctccgagag cggtgtgggg tctcgcgcgg
     1681 cggccgttcc gccccgcaaa acagcgagcg aacgatatag tgctcgtgct ccggcgttgc
     1741 aagccgcgct cgacgagcga agccgttgca gcgaagcgcg cgccgaggca ctctcgcgcc
     1801 catgttgctc gacgagatca aggcgcgcat gttcaaagcc atgaaggccg gcgatgggct
     1861 cgaaaaagag atcctgcgcg tcgccatcgg cgaaatcacg acggattccg cgcgcccggg
     1921 gcgcaaggga aatgacgacg aagcgcaggc gattttgcgc aaactcgtca agtcgaacga
     1981 agagacaatc gcgtcgctcg aagagggaga gaagaagtcg tccctcttgc gcgaaaatca
     2041 gattctgagc gacttcttgc ccaagtcact cagcgaagac gaagtcgtcg ccgcactcgc
     2101 gcccgtcatc gaacaagtcc gcgccgccgc gaacgatggc caggccaccg gcgtcgccat
     2161 gaagcacctg aaggcgagtg gagccctcgt ggacggcaaa gtcgtcacca aggcggtcca
     2221 gcgcgtgcgc gcgggctgaa cgccgcgttt ccaccccacg cggggcgagc ggccgcggtt
     2281 gagctcgccg ctcgttccgt gcccccgggg gcaccggcac cgcgctccgc aaataccgat
     2341 ggaggacggc gagggacggc tcgtcgagcg actcgaagct caatcccaag taacgagtgt
     2401 gctcacccgc gcgacgaccg tgcacgacgc gcgcgaccac gacctccgcg tctacgtata
     2461 ttccgtggcc gacgcagaac gaaagtagga ggcgctcacc cgtcaggacg ggatccgccg
     2521 gtcccaccag cgctccgcct ggcgataggt tcagaatgcg gtctgcgacc aaccggaagt
     2581 cccgttcgcg gacgacctga caagtgagag gagccgtgta gcgagcgggg cgttttgcgc
     2641 gccgagcgaa gcgagacatt aaccctccgg gcgttggctc ctcaaatgct agggaaccag
     2701 cagcgtgcgg gccaaattcc aagagcattg gaggagcggg gcgaaactgc ccgctggggc
     2761 ggcgacaatc gcctggaaga ggtcgcacgc aaatcgccgc ggcatagtat attgttcgat
     2821 cgaggattcc ctcgatgagc cgctatccgc gcgcaacgcc atccgccccg atccccaagg
     2881 cgagcgttcc cccagcagac cgacggctat cagcggtctt ggtcgccccg gtcgtagtcg
     2941 tcgcgccgga cgtcgagcgc gagctcattt caggctcgct ccgcatcggg cggcacccga
     3001 gctccgacat cgtcttggaa gacgcgctgg tctcgcgtac ccacgcgcgc ctcgtcgtcc
     3061 aagaggacgg ctccttggtc atcgaagacc tgcacagcgc gaacggggtt tacgtcaacg
     3121 gcgagcggct cggtcgccat tcacatcggt tgtgcgaggg cgaccggctc ttgattggta
     3181 cgtgcgagct cagtgtcttc tcttgccggg cagcgcccag cttggccgaa ccgcccagcg
     3241 tccgctcggc catcgcgcgc gtgctcgaca ccgacgccga aggcccgccc acgaccgagc
     3301 gcaccgatgg cctacaaatg gtcgctcgct tggccgaacg ttttcaccgc gccggcaatt
     3361 cgatggaagc gattcgcgtg ctgtcggggc acctgaacaa ggtgctgctt ggcgcgtccg
     3421 cgggtctcgc ggtaccgagc gagctcctcg atgaagcttc tcgccacgcc gtcgagctct
     3481 actactggac gtacaacggc gcctggatcg actacgtcat cgagctccac cttgcgaccc
     3541 aagcgctccc gagcgagcgc accgtagcga cactcgaggc tgccatcgcg tcctcgagcg
     3601 ggactccgtt cgacgccggg ctgctcaagt acttcctcga gagccaggcg agcacgaaag
     3661 caccgcgcac gctcgcagaa gaggcgcggc tctcgcgact tgagcgcctg cgctcccagc
     3721 gctgacccgc gcgccgcgtt tgcggcccag ccctcgtcgt gaaggctggg cgccgcggta
     3781 cagctcagcc ggtgagctgg gacagcgcgc cggtcgtgag cttcgaatcc ggctgcgtgc
     3841 cgaacgtatt gacgtcgacg ccaaaggcct gcgcgatgga caccaaaatc ttgttgttgg
     3901 tgaccggagt gaacaccgca tcggcttcgc cgcaccacgc gttgctggct gggcagtccg
     3961 cctgcatctt gagccgccgg cccatcttga acttgccgtt cacgccgccc gcgagcaggg
     4021 tcgggacgtt gcgcgtgctg tggagcgccg ggtttcccat gtcgctcgaa gcgtagatga
     4081 gcgtgctgtc gagtacgccg agttgatcga gcttctgcat gaagcgcgcg atcttcccgt
     4141 acgagtattt gttgaatttg gcgagcggag tccaggtcga cggcgtgcca gccccgaccg
     4201 gagcgccgtt gttgccaatc ggtgacgccg cgtaggtatg cgcgacgctg ccgtggttgt
     4261 ccgccgggag cccgagcggg ttgccatcgt aggagaggtc gttcaggaac acggtcgcga
     4321 agcgagtgat gtcgcacgcg aacgcttgcg ccagtagatc cagctggatg tcggtgatgg
     4381 cgtcgaagta gggctcgccg ccgttgtagc gctggagctt ggggaacttc gtcgcgtccg
     4441 gcttcgaggg caccgcacag ccgctcatgg tcggcgactc gaattgcttc tcgatctcgc
     4501 gcaacgccgt caggtgttgg tcgagcttct gctgctcggg tgctgccaag cgccctcgca
     4561 ggcgattgat gtcgccgcgc acgaagtcga tcacgctctg cccgttcttg ttctcgcggg
     4621 cgagcgcggc ttgcgcagca gagtcgttgc ccgccgcaaa tccggcgaag agctgcttga
     4681 aggtctcggt cggatcgatg atcttcgcga gaggcgcgcc gcccggacca aaggagaggc
     4741 actcacccga actcagcgtg gaggttccaa ccgcgagcgc gatgctcgcg atgcgagtct
     4801 ggctgccgag ccccttctcc acggctagga actgatcgag cgagctgttg agggccttct
     4861 tgttgccgtc gatgcggctt ccggtgagaa tcgtccccgc cgagtcgtgg ccattcgcgt
     4921 tcgacaaatg gtcgatgccc tcgacgacga tgatcttgtc tttgaagctc tttccgtagg
     4981 tggcggcgtc gtcgaagggc ttcaacgagc agtccgcgta actgaagtcg aacttcgtct
     5041 ccgtgtcgga gtctttcatc gcccagagct cggccgctac gccgtgggga tgatagatgc
     5101 cgacgaactt cagcggcagc gtatcgcccg ccgcctgcgc atacgagttc tcgagcaagc
     5161 ggaagaaggg caacgtcgcg aacccggcgc ctacggcgcg caagaacgcg cgacgattcc
     5221 tgtttttgat cacggcgccc tccgatgcgt aaagagcggg cttttcacga acgtggcgag
     5281 cgtctgcaag atgtttccct gctgatccat tggcaacgat tgccagtatc tgatgaaggc
     5341 gtcttcgctg gcttgaacgg tgtcgtcgct gcgcgcggcc gagccgcgga acatgtgtcg
     5401 cgcaaagcac gccgtcactt gcgggctctt cgacatggcg accgcgagcg cgttgccgtc
     5461 agcgtagtcg ccatcgaagt cggtccctga attgatggtg accttgctgt tgacgtcgtt
     5521 ggacccctcc ttcgttcgga aggtgcccat gccgtcgtac tcctcgaatg cgaagccgaa
     5581 ctggtcgatg ttcttgtggc agccggcgca gatcgagtcg gtagagtgcg tcgaaaagcg
     5641 agcgcgcgtg gtcttgctcg gatccggagg tggcggcacg accgctatgt tgagggtggt
     5701 cggcgaggcg atgttgagac acgcgaagcg gcgcgcgata tccaccccgc gcagcacggg
     5761 gctgctctcg tgcgcgtggg caaacacgga gagccacgcc gcctggttca ggatgcctcg
     5821 acgagtgggc agcgtgacgc ggcccgagcc gttcgcgttg tagagctgcg caagcttggt
     5881 atcggtcacc atcgtccagt tcgcaccgag gagctcaccg accgtgccgg tgccggtgag
     5941 cacattttcg acgaattgct ggctctccgt caccatcgcc gccttcaggc cggcgaagtt
     6001 cgggtaaatg ttgctgtcct tgtccgtctc cgagatgcgg tcgacaccga cccattcgcg
     6061 cacgacgcga acgatgcggt cgcgcgcggc cggcgtgcgg accaggtcgt cgaacgcctt
     6121 gccgcgtccc tccggcgtgt cgagcttgcc ggcgacggcg tcggcgacga gctgctcgct
     6181 cggcggcgcg ttggtgacca agaaagagag cgagcttgcg agctcgtagg gcgtcagcgc
     6241 cgcggccgtt cccggcgccg cgacccccgc gttaccgatc tcggtgagat agagaaagcc
     6301 cgccgagttc accaaggcgc gcacgacgag ccgaatgcca tcggcgtagg tcgcgccgtc
     6361 cgcgccggtc ttgtagacgg cgagcaggcc cgcgctctct tccgtggtca gcggccgacg
     6421 gtaggccttg gcgccgtagc tcgcaatgaa cgactgagcg cacgcctccg ccttgctcgt
     6481 gtcctggcaa ggcgcgttct tcgttacatc gaagctcgat gcagcatcgc tggcggcggc
     6541 gtagatttgc ttcgcggtga cgttgtcgac gcgctgagcg tcgttcagcg tgaaaccgct
     6601 ctgccgcgcg tcgggcgcga agctttgagc cggcgctttg tcggtgccgg tcaggttctt
     6661 cagcgacgca tcgaactccg cgttggcgag ccgacggatg cgctgcggaa tcatctcgga
     6721 gggttcgacg ccagacggta acggcgtcga gccaccgctc gtcatcgggg gagaaccagc
     6781 ggtgcttccg ctgcctccgc cggggttgct cttgccacca ctcggattcg cgccaccgcc
     6841 attgctgcta ttgccgctgc cgccctgcga aacgccgcta ccggttccag cgttcggctg
     6901 cgccgaaccg cccgtctc
//
LOCUS       DASTKS010001074         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1732220, whole genome shotgun sequence.
ACCESSION   DASTKS010001074 DASTKS010000000
VERSION     DASTKS010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732220"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFQ35_22635"
     CDS             <1..838
                     /locus_tag="VFQ35_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9933527.1"
                     /translation="LLVAAGSGAATFGNPDSAGVPASIVEHTATLSYNDVPALEELFA
                     RRGNDIACVIVEPVAGNMGCVPPAPGFLQAIIDLCKKHGALSIFDEVMTGSRLSQSGA
                     QGRFGMRPDLTCLGKVVGGGMPLAVYGGRRDVMSLVAPLGPVYQAGTLSGNPLAVSAG
                     LATLAELTPSAYEALERTSQTLAEGMRKAIEKTRQKAVVQSVGSMLTLFFHDGPVKNW
                     NDAKKSDTKRFAAFHQAFTARGIYWPPSQFEAAFVSLAHTSDDVEKTLAAAEAALGTL
                     QG"
     gene            complement(829..1842)
                     /locus_tag="VFQ35_22640"
     CDS             complement(829..1842)
                     /locus_tag="VFQ35_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9933528.1"
                     /translation="MSRGAAQCVLAAILALATGACSERSDEHATGCPTKTTSGAVHVS
                     NGIGTLGGALTLPAGCGPFPVLLIIAGSGATDRDGNQLSAGVRPDTYLKVANALAPSG
                     VATLRFDKAGVGESLGAAPKQADQRFEDVVADAALWLDWLRADSRFDAVIVAGHSEGA
                     LVGLLLARSKPVEAYVSLEGAGRPIDVILREQLAQNTGLSADVLTAANSILDELLQGR
                     AVASVPAELAWLFAPALQPYIMSWMRYDPAAEFAKTTMPALVVQGTADTQVSLVDAEL
                     LSKARPDARELIVEDMSHFLKPATLDVDSQMRSWRDPSLPVRSEVIDAIRALVNEVAP
                     GQP"
     gene            complement(1853..2509)
                     /locus_tag="VFQ35_22645"
     CDS             complement(1853..2509)
                     /locus_tag="VFQ35_22645"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HET9933529.1"
                     /translation="MRELLNRVRAQRETQVYSELWGASSQTFSFDEQFFQRTFQQAAD
                     PHWLLASVIEWAPNEKHSDWVYVSSGLSNPLDDAEPEVVDDFSWLGVEVMFRSKEQGL
                     WAVQLVQRVAAFEILLAYERFPGRDRLAPGARIPLGGPIIPESDSQLKWLLVVPPMEN
                     AVSFDLESGRAEFLNIVGITEGEAKVAREQGAEVLFEQLRNHGALWVTDPRRGSLVAD
                     "
     gene            2863..>3187
                     /locus_tag="VFQ35_22650"
     CDS             2863..>3187
                     /locus_tag="VFQ35_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933530.1"
                     /translation="MKLRALITLTAGLSLSTLMLACSSDGGGNSGNGGTPGQGGSTAN
                     GGSANGGSSSGGAASGGAASGGAASGGAASGGASSGGASSGGASSGGASSGGKSSTGG
                     GSSGGA"
BASE COUNT          590 a         1085 c          968 g          544 t
ORIGIN      
        1 gctgctagtc gccgccggca gcggcgccgc cacgttcggc aaccccgact cggcgggcgt
       61 gccggcgagc atcgtcgagc acaccgcgac tctctcttac aacgacgtcc ccgcgctcga
      121 agagctcttc gcgcgccgcg gtaacgacat cgcgtgcgtc atcgtcgagc ccgtcgccgg
      181 caacatgggc tgcgtcccgc cggcgcccgg ctttttgcaa gcgatcatcg atctctgcaa
      241 gaagcatggg gcgctcagca ttttcgacga ggtgatgacc ggctcacgtc tctcgcagag
      301 cggcgcgcag ggtcgctttg gaatgcgccc cgacctcact tgtctcggca aggtggtggg
      361 gggaggcatg ccgctcgccg tttacggcgg ccggcgcgac gtgatgagct tggttgcgcc
      421 actcggcccc gtgtaccaag ccggcacgtt gagcggcaat ccactcgcag tgagcgccgg
      481 gctggcaacg ctcgcagagc tcacaccgag cgcgtacgaa gcgctcgagc gaacgagcca
      541 gactctcgcc gagggcatgc gcaaggccat cgaaaaaacg cgccaaaagg cggtcgtgca
      601 gagcgtcggc tcgatgctca cgctgttctt ccacgacggc cccgtgaaga actggaacga
      661 cgccaagaag tccgatacca agcgctttgc cgcgtttcac caggcgttca cggcgcgcgg
      721 catctactgg cccccgtcgc agttcgaagc cgcgttcgtt tcgctggccc acacgagcga
      781 cgacgtcgaa aagacgttgg ccgcggcaga ggccgcgctc ggtacgcttc agggctgacc
      841 aggagcaact tcgttcacga gcgcgcggat cgcgtcgatg acttcgctcc gtacggggag
      901 cgacggatcg cgccaggaac gcatctgcga gtcgacgtcc agagtcgctg gcttcaagaa
      961 gtggctcatg tcctcgacga tgagttcgcg tgcgtcgggg cgcgccttcg aaaggagctc
     1021 cgcgtccacc aacgaaacct gcgtgtcggc ggtgccctgc acgacgagcg cgggcatcgt
     1081 cgtcttcgca aactccgcgg cagggtcgta gcgcatccaa gacatgatgt agggctgcag
     1141 cgccggcgca aacaaccagg cgagctcagc gggcacgctc gcgaccgcgc gtccttgaag
     1201 tagctcgtcg aggatcgagt tcgcggccgt caaaacgtct gccgaaagcc ctgtgttttg
     1261 cgccaattgt tcgcgcaaga tcacgtcgat cggcctgccc gcgccctcga gtgagacgta
     1321 cgcttcgacc ggcttgctcc tcgcgagcag caagccaacc agcgcgccct cgctgtgccc
     1381 cgccacaatc accgcgtcga agcgagaatc tgcgcgtaac caatcgagcc agagcgcggc
     1441 gtctgcgacc acgtcttcga atcgttggtc cgcttgcttt ggtgcggcac ccaagctttc
     1501 gccgacgccc gccttgtcga agcgcaacgt cgcgactcca ctcggtgcca gcgcattcgc
     1561 gactttcagg tacgtgtcag gtctcacccc cgcgctgagt tgatttccgt cacgatccgt
     1621 cgcacccgag ccagcaatga tgaggagtac cggaaacggg ccgcaccctg ccggaagagt
     1681 gagcgcaccg cccaaggttc cgatgccgtt gcttacgtga acggcaccgg aggtcgtttt
     1741 cgtcgggcag cccgtggcgt gctcgtcact tctttccgaa cacgccccag tcgccagcgc
     1801 cagaatggcc gccagcacgc attgcgcggc gccacgagac atcgtgcggc tctcagtccg
     1861 cgaccagcga gccgcgcctc ggatccgtca cccagagcgc cccatgattg cggagctgct
     1921 cgaacaacac ctcggcacct tgttcgcggg cgactttcgc ctccccttcc gtaatgccga
     1981 cgatattcaa aaattccgcg cgtccggatt ccagatcgaa gctcaccgcg ttttccatcg
     2041 gcggaaccac caagagccac ttcagctgcg agtcgctttc ggggatgatc ggaccgccca
     2101 acggaatgcg cgctccgggt gccaacctgt cgcggcccgg aaagcgctcg taagcgagca
     2161 gaatctcgaa agccgcgact ctttgcacga gctgcacggc ccagagcccc tgctctttcg
     2221 agcggaacat cacctcgacc ccgagccagg aaaaatcatc caccacttcc ggctccgcgt
     2281 catcgagcgg attggacaaa ccggacgaaa cgtagaccca atccgaatgc ttctcgttcg
     2341 gtgcccattc gatcaccgac gccagcagcc aatgcggatc tgccgcctgt tgaaacgtgc
     2401 gctggaagaa ctgctcgtcg aacgaaaacg tttgcgagct cgcgccccaa agctccgagt
     2461 aaacctgcgt ttcgcgctgt gcgcgcaccc tgttcagaag ttccctcatg tcaccaccgg
     2521 cccacagtct aagccacatt caaaaacgcg ggaaaccccc ttcgcgaaaa acttctcgcg
     2581 tccttcgaat ttgggcaatt ccgtactcgg tggggtagcc cgggcgccac atgcgcgtca
     2641 cctttcgatc gctcgcgagc tcgaaactcg cgccgcactt ttctgaccgg atcgaggggc
     2701 gcggcggatc tgctcgcgaa ggccaatccg actcgatgtg aacggccagt aactagaacg
     2761 atcgatcgac tctttcgatc ccccgtcgat ttcgaggttc ctttcaggcc actccacgct
     2821 taagacagcg gcgcgacggc ggatcacttc attggaggac acgtgaaact tcgagcactc
     2881 atcactctta cggcgggact ctctctctcc accctcatgc tcgcttgcag ctccgacggc
     2941 ggcggcaact ccggcaacgg cggcactccg ggccaaggcg gaagcactgc aaacggcggc
     3001 tcagccaacg gtggcagcag cagcggcggt gcggcttcgg gcggtgcagc ttccggcggt
     3061 gcagcttccg gcggcgcggc ttcgggcggc gcaagttcgg gcggtgcgag ctccggcggc
     3121 gcaagctccg gcggcgcaag ttcgggcgga aagagcagca ccggtggcgg cagcagcggc
     3181 ggtgccg
//
LOCUS       DASTKS010001075         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1957136, whole genome shotgun sequence.
ACCESSION   DASTKS010001075 DASTKS010000000
VERSION     DASTKS010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957136"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFQ35_22655"
     CDS             complement(<1..219)
                     /locus_tag="VFQ35_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933531.1"
                     /translation="MQGSSIVIADDDRLTRDAVCNVLRSHGYVVDAVGDGQAAVERVA
                     RGGVDLVLLDILMPRLSGLEACRLIKGMT"
     gene            complement(400..2205)
                     /locus_tag="VFQ35_22660"
     CDS             complement(400..2205)
                     /locus_tag="VFQ35_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933532.1"
                     /translation="MRRSLTVLAGCVGLSAAAPAWAEPPPAPAEHRVDFRADQVELET
                     DARSLDLQGNVVIRSERFRLTGERLSLARSPRGVHVEGAGVLALCPCEKPPIAFGLRS
                     ADLAPPSDVLMKSATLRVFGVPIFYWPYLWLRSPNRTGVLPPSVAYRGEEGLLLGSGL
                     HVPLKSAPNASIDLNAAGYVRGGARLEAQLRKPGARVDVAFDQFRGSALDLRSTASSA
                     GENGSFAALRADWLAGARGQSGTSALERVVLPSDRFHAAIGRAGSGVLAFSLRADAPR
                     SALLGELGWAGPGLGVGMGGALGRRARYAVFAETQSLQSSREGALIARAGGELSASFA
                     FGPMVTSASSRERLSYVSGSERVSRELVHETRLRFALPLARRYGALIHGVAPFAEASL
                     MAGSRALRADDPMSTVLTAPMQGYRLVTGVSSSLGAPLDRAGSAELLGGLSGDGARSE
                     PVLAGRLRADAAWVRASAEARALPEHSSADALLRLELGAEDSLRVGGHVEGARGDVTA
                     ASGVFSDDFSLPANARFDRSGFSGGARVHVPFGPRISTSGGAEFDVSRSEVLAWWGQL
                     RYRHPCRCMSLSVLGAHRTGRSGVDVGVNLELIPQ"
     gene            complement(2206..>2796)
                     /locus_tag="VFQ35_22665"
     CDS             complement(2206..>2796)
                     /locus_tag="VFQ35_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933533.1"
                     /translation="EGAVLSSVLLRPRATYTYQFVSSPVATRSGFDRIRLDPEGRSLP
                     NDQTSGSALPQHLLNLSLRVDTTITDRVRVITEVGMRYARRYALPGSVPIQNVDTGPV
                     MPQQSPQPARWDVITLFSTGVYYAITHSAEVGIGYENLAGQLGPDGRRRSFFYSPDAR
                     VFLALVVPLGELYHDVRHEQAPEPNHTASAATQSVF"
BASE COUNT          508 a          973 c          938 g          377 t
ORIGIN      
        1 cgtcattccc ttgatgagcc ggcaagcctc gagccccgat aggcgcggca tcaagatgtc
       61 gagcaggacg agatcgacgc cgccgcgagc gacgcgttcg accgccgctt gcccgtcccc
      121 caccgcgtcg accacgtagc catgcgagcg cagcacgttg cacacggcat cgcgcgtgag
      181 gcgatcgtcg tcagcaatga cgatgctcga cccctgcact ggtccgcccg gggactcgct
      241 tctggaacca ctctccgaca cgaaaaccta gcgtacccga agcgcaatcc gttgacgaca
      301 gcgcgcatgc acgaagagcg gcgtttttcg gagctttgta cgccgccgct ttgtcccaca
      361 ccgacgccta cggtggtcgc ggcacgagca tgcgcgcagt cattgaggaa tcagctccaa
      421 attgacgccg acatccacgc ccgagcggcc cgtacgatgc gcaccgagga cggagagcga
      481 catgcaacga caggggtggc ggtagcgaag ttgcccccac caagcgagca cttccgagcg
      541 gctcacgtcg aattcggctc cgccactcgt cgagatgcgc ggcccgaacg gaacgtgaac
      601 gcgagcgccg ccgctgaagc cgctgcggtc gaagcgcgcg ttggcgggca acgagaagtc
      661 gtccgagaac acgccgctcg cagccgtgac gtcgccgcgt gcaccttcga cgtgaccacc
      721 gacacgcaac gaatcttccg ccccgagctc caggcgaagc agcgcgtcgg cggaggagtg
      781 ctcgggcagc gccctcgctt cggcggaggc tcgcacccac gccgcgtccg cgcggagccg
      841 tcctgcgagc accggctcgc ttcgcgcccc gtcaccgctg agcccaccca agagctctgc
      901 gctgcccgcg cgatcgagcg gagcgcccaa gctgctcgaa acgccggtca cgagacgata
      961 gccctgcatg ggagccgtga gcaccgtgct catcggatcg tccgcacgca gcgcgcgtga
     1021 ccccgccatg agtgacgcct ctgcgaacgg cgcgacgccg tggatcagag cgccgtagcg
     1081 gcgcgcgagc ggtagtgcga agcgaagccg cgtttcgtga acgagctcgc gtgacacgcg
     1141 ctcggagccg ctcacgtagg agagccgttc acgcgaactt gcgctcgtga ccatgggccc
     1201 gaacgcgaac gacgccgaaa gctcgccgcc agcgcgcgca atgagcgctc cttctcggct
     1261 ggattgcagg ctttgggtct ctgcgaacac ggcgtagcgc gcgcggcgac cgagcgcccc
     1321 gcccatcccc acgccgagcc cgggccccgc ccacccgagc tctcccaaga gcgcggagcg
     1381 cggggcatcg gcgcgcagcg aaaacgccaa aactccgctg ccggcccgac cgatcgcggc
     1441 gtggaaacga tcgctcggca gcaccacccg ttcgagggcc gaagtcccgg attgaccgcg
     1501 cgctccggcg agccagtcgg ctcgtaacgc ggcgaacgag ccattctcgc cggcgctcga
     1561 cgcggtggag cgcaaatcga gcgcgctgcc gcggaattgg tcgaacgcga catcgacgcg
     1621 cgcgccgggc ttgcggagtt gcgcctcgag gcgtgccccg cctcgaacgt acccggcggc
     1681 gttcaggtcg atggacgcgt tcggcgcact cttcaacgga acgtggagcc cgctcccgag
     1741 caaaagccct tcttcccccc ggtacgcgac ggacggcggc agcacgccgg tgcggttcgg
     1801 cgaacgaagc cagaggtagg gccagtaaaa gatggggacg ccgaaaaccc gcagtgtcgc
     1861 ggacttcatc agcacgtcgc tcggcggcgc gagatcggcc gaacgcaggc caaacgcgat
     1921 gggtggcttc tcgcaagggc agagcgcgag cacacccgcg ccctcgacgt ggacgccgcg
     1981 cggcgaccgg gccagcgaca gacgttcgcc agtgagacga aatcgttccg accgaatcac
     2041 gacgttgccc tgcaaatcca gcgagcgagc gtcggtctcg agctcgactt ggtcggcgcg
     2101 aaagtcgacg cggtgctcgg cgggtgctgg cggcggctct gcccacgcgg gagctgccgc
     2161 gctgagccca acgcagccgg ctaagacagt gagcgaacgc cgcattcaga aaacggactg
     2221 agtcgccgcg ctcgccgtat ggttcggctc gggcgcctgc tcgtgccgaa cgtcgtgata
     2281 gagctccccg agcggcacga cgagcgccaa gaacacgcgc gcgtccggcg aataaaagaa
     2341 gctgcggcgg cgcccgtcgg gcccgagttg ccccgcgagg ttttcgtagc cgatgcccac
     2401 ctcggccgag tgagtgatcg cgtaatacac gccggtcgag aagagcgtga tcacgtccca
     2461 gcgcgccggc tggggcgatt gctggggcat gaccggcccc gtgtcgacgt tttgaatcgg
     2521 caccgacccg ggcaatgcgt aacgacgcgc gtagcgcatg ccgacttcgg tgatgactcg
     2581 tacgcgatcc gtgatcgtcg tgtcgacgcg aagggacagg ttcaacaggt gctgcggcaa
     2641 cgcggagccg ctcgtctgat cgttcggcaa cgagcgtccc tcgggatcga ggcggattcg
     2701 gtcgaagccc gagcgagtcg caaccggcga gctcacgaac tggtacgtgt acgtcgcgcg
     2761 aggccggagc agcacgctgc tcagcacggc gccttc
//
LOCUS       DASTKS010001076         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1732376, whole genome shotgun sequence.
ACCESSION   DASTKS010001076 DASTKS010000000
VERSION     DASTKS010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732376"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFQ35_22670"
     CDS             complement(<1..494)
                     /locus_tag="VFQ35_22670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9933534.1"
                     /translation="MSTERYDVIVVGGGPAGSTAATLLARRGKRVLVLEKESFPRFHI
                     GESLLPCSIPLFEQLGLLPELERRFLPKYAAEFVTSDGKLARRYTFEHGMVKGPISAY
                     EVDRAELDQLMLDNAKAAGADVRQSTQVTDFDVNERGARVTARTADGCHHGYEAEMLL
                     DASG"
     gene            complement(535..1608)
                     /locus_tag="VFQ35_22675"
     CDS             complement(535..1608)
                     /locus_tag="VFQ35_22675"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HET9933535.1"
                     /translation="MIESKEPHEPPHATSELPPTGGAIGSEPEDFCVEEIPAYLPTGA
                     GEHVYVEVRKRRLSTQDLVRRVAEAAAVDSRDIGYAGLKDKHAVTTQWLSLPGRAKPT
                     SQWSLPTGIEVVRESRHNNKLRTGHLKGNRFRITLRGVASDGLERAQAIVKELEQGGV
                     PNYFGAQRFGRGGENLARAIEFLAGGARQRLPPFLLKLYPSVFQSEIFNRYLTLRREA
                     SLDRLFSGEVVRLEGSSASFVVEDSEKEAPRLAAKEIHLTGPMLGPKMRPASGFVQEL
                     ETKAFASLGLDPGILDVLGRFAPGARRDLLVFPEGASVTAPAPDELVLEFGLPAGSYA
                     TVLVRELTKRPLLGGRDELERDA"
     gene            complement(1611..2576)
                     /locus_tag="VFQ35_22680"
     CDS             complement(1611..2576)
                     /locus_tag="VFQ35_22680"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9933536.1"
                     /translation="MTTELVLRAFGDTDTGRVRANNEDCVLVRPDLLLYVVADGAGGH
                     NAGEVASRLAVRSIENFFGATIRATHDRPEFDRFGIPSGARRLSQAILKANHDVIEIA
                     KSSVPHRGMGTTVVAACFSPRSGLLHVGHAGDSRCYRLRSGHLELLTQDHSLVTDIIE
                     QRPDIDDVVLDRLPKNMVTRALGMDPRLRVSMRSHAVVPGDKYLLCSDGLSSMLSAEE
                     LAETLSREESAQLLVERLIAIANAAGGTDNIAALVIESQGGHEHARPADSVPPAAPGA
                     SPGAGADRLSDPELLILGIEDLDAAGVDRNEGLMRDLNELFGRTR"
     gene            2770..>3078
                     /locus_tag="VFQ35_22685"
     CDS             2770..>3078
                     /locus_tag="VFQ35_22685"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9933537.1"
                     /translation="MRSLVPANSKTGLVLSGGGMRGAYEVGVVSGILEVLKPKPGEAP
                     VFRVFSGASVGAINTAYLAANAELADHGIGRLVDLWRSLKLADHARIRPLGLAQWPQS
                     L"
BASE COUNT          557 a         1011 c          985 g          525 t
ORIGIN      
        1 tgcccgctcg cgtcgagcaa catctcggcc tcgtatccgt gatgacagcc atcggcggtg
       61 cgcgccgtaa ctcgagctcc gcgctcgttc acgtcgaagt cggtgacttg cgtgctctgc
      121 cgaacgtcgg cgccggccgc cttcgcgttg tcgagcatca gctggtcgag ctctgcccga
      181 tccacctcgt acgcggagat ggggcccttc accatgccgt gctcgaacgt gtagcggcgc
      241 gcgagcttgc cgtcggaggt cacgaactca gccgcgtact tgggtaagaa gcgacgctcg
      301 agctccggca acaagccgag ctgctcgaag agcggaatcg aacacggcag cagcgattca
      361 ccgatgtgaa agcgcggaaa gctttccttc tcgagtacga gaacgcgctt gccacgccgc
      421 gcgaggagcg ttgctgccgt cgacccggcg ggtccgccgc cgaccacgat tacgtcgtag
      481 cgttcagtgc tcatcgttca ccggctcaca ctaaactcga gccgccgcga gcgttcaagc
      541 gtcgcgctcg agttcgtctc gaccgcccag cagcggacgc ttggtgagct cgcgcacgag
      601 caccgtggcg taactgccag ccggcaaacc aaactccaag accaactcgt ccggcgccgg
      661 ggcagtcacg ctcgccccct cgggaaacac gaggaggtcg cgccgagcgc ctggggcgaa
      721 acggcccagc acgtcgagaa tgccgggatc gagaccgagg cttgcaaaag ccttggtttc
      781 gagctcctgg acgaacccgc tcgctggccg cattttcggg cccagcatcg gaccggtgag
      841 gtgaatctcc ttcgcggcaa ggcggggcgc ctccttctcg gagtcttcga ctacgaagct
      901 cgcagaggaa ccttcgagcc gcacgacttc acccgaaaag aggcggtcga gtgacgcttc
      961 gcggcgcagc gtgagatagc gattgaagat ctccgactgg aaaacgctcg gatacagctt
     1021 caaaaggaac ggaggcagcc gctgccgcgc tccacccgca agaaactcga tcgcgcgcgc
     1081 aagattttcg ccgcctcggc cgaagcgctg cgcgccgaag tagttcggca ctccgccttg
     1141 ttcgagctcc ttgacgatgg cctgagcgcg ctcgagccca tcgctcgcga cgccacgcaa
     1201 cgtgatgcgg aaacgattcc ccttcaggtg accggtgcga agcttgttgt tgtggcgcga
     1261 ctcgcgaacc acctcgatcc cagtcggcag actccactgc gacgtcggtt tggcgcgccc
     1321 gggcagcgat agccactgcg tcgtcaccgc gtgcttgtcc ttcaacccgg cgtaaccgat
     1381 gtcgcgagag tcgaccgccg ctgcctccgc gacccggcgc accaagtctt gtgtcgacaa
     1441 gcggcgcttt cgaacctcga cgtacacgtg ctccccggcg ccagtaggca gatacgcagg
     1501 gatttcttcg acgcaaaagt cttccggctc ggagccgatc gcgcccccag taggcggaag
     1561 ctctgaggtc gcgtggggcg gttcgtgcgg ctctttcgat tcgatcatgg tcaacgagtt
     1621 cgcccaaaaa gttcgttcaa gtcgcgcatc aggccttcgt tacgatcgac cccggccgcg
     1681 tccaaatctt cgatgccgag gatcaagagc tctgggtccg acaagcgatc cgcccccgca
     1741 ccggggctcg cgcccggagc agctggcgga acgctatcgg ccgggcgagc gtgctcgtga
     1801 ccgccctggc tctcaatcac caacgcggcg atgttgtcgg tgccgccggc ggcgttggcg
     1861 atcgcgatca gccgctcgac gagcagctgc gcgctctctt ctcggctcag cgtctcggcg
     1921 agctcctccg cgctcagcat gctgctcagc ccgtcgctgc aaagcaggta cttgtcgccg
     1981 ggcaccaccg cgtgcgagcg catcgagacg cggagccgtg gatccatccc gagcgcgcgc
     2041 gtgaccatgt tcttcggtag acgatcgagg acgacgtcgt cgatgtccgg ccgctgctcg
     2101 atgatgtctg tcacgagcga gtgatcttga gtcaggagct cgaggtgacc agagcgcagc
     2161 cgatagcagc ggctgtcgcc ggcgtggccc acgtgcaaaa ggcccgagcg cggcgagaaa
     2221 cacgcagcga cgacagtagt gcccatgcct cgatgcggca ccgagctctt cgcgatctcg
     2281 atgacgtcgt ggttcgcctt caagatcgcc tgcgagaggc ggcgcgctcc cgaggggatg
     2341 ccgaagcggt cgaactccgg ccgatcgtgt gttgcgcgaa tggtggcgcc gaagaagttc
     2401 tcgatcgagc gcaccgcgag ccggctcgcg acttcgcccg cattgtgtcc accagcgccg
     2461 tcagccacga cgtagagaag gagatcgggc cgcacgagga cgcagtcttc gttgttggcg
     2521 cgcacgcgcc ccgtgtcggt gtctccgaat gcgcgaagca cgagctcggt cgtcatgatg
     2581 agggagcgag aagcgagtag gcttttcgca cgccgccccg aaccggtcga ggcccaagcg
     2641 gtcgaagcca atcccgaacg ccgaaaattg cacgaaagac gtggagaaag gtgcgcgaga
     2701 agcgccgcac gcacgcgatt acccttgacc taaccgcgcg ttccgaaaag actaaaaagt
     2761 cggagcttga tgcgatcgct cgtgccagcg aactcgaaga cggggctcgt gctctccgga
     2821 ggcgggatgc ggggtgcgta cgaggtcggc gtcgtctcgg gcatcctcga ggtgttgaag
     2881 ccgaagcccg gtgaagcacc ggtttttcgg gtattttcgg gcgcgtccgt cggtgcgatc
     2941 aacaccgcct acctggcggc aaacgccgag ctcgccgacc acggcatcgg gcgcttggtc
     3001 gatctctggc gttcactcaa gctcgcggat cacgcgcgta tcaggcctct tggcctggct
     3061 cagtggcccc aaagctta
//
LOCUS       DASTKS010001077         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_169825, whole genome shotgun sequence.
ACCESSION   DASTKS010001077 DASTKS010000000
VERSION     DASTKS010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169825"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFQ35_22690"
     CDS             <1..359
                     /locus_tag="VFQ35_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933538.1"
                     /translation="IETGHEHSYERTKTLTDMALQTVDPTCSTPGTLCVGPGRTFSSV
                     VGLGGNSVRDQSRCLPSTYPYGCNGEWAFIYTNQQSATHGAQFITFNAGSAKTAVGYF
                     KNINGATVDTFTIQHD"
     gene            352..1839
                     /locus_tag="VFQ35_22695"
     CDS             352..1839
                     /locus_tag="VFQ35_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933539.1"
                     /translation="MIDVRKLRGSMSLERRGPSLVVLSGVLAHATALGAGFVWLDHAH
                     IEGGLGWARPSEWASLFTRPFAGTGFYRPLMALALSLDMAVSPTAPFMHAVTLAWHAL
                     AAAFVTWTARELGSSTRASLLAGMAFAVHPMNSLVASAISFRSEAMSLVFLLALVLSH
                     LRTRPVAAALAMAACGLTKETSWLLGPLFVTALTLRSRQDSWRSTTWGKRRLLLAEAF
                     SFLAVSLLRLRFAPAWRADYPALAWDEQIGSRLALLGKGFARALLPLPNTLCDAFPVV
                     SLPSAFSVLGLALLTLLFATRAASPVAKSLLLLSLLPCVELVPIMRWWSPHYFYVPLA
                     LAAIAFSDAAERRWGRSPWLVLVALGAVAFSLRDALRFRDDESLWSYEVAHEPACREG
                     HFYLGEVARERGDLTRAARHYRSAAAPVPGFLAYSDEFSAYQNWGAVELASGHFAEAR
                     AAFAEARERGQTQRQRAEASHNLAVVAVALGEARTGAALLAGTPE"
     gene            1900..2727
                     /locus_tag="VFQ35_22700"
     CDS             1900..2727
                     /locus_tag="VFQ35_22700"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET9933540.1"
                     /translation="MTAKSCAFGIFLLFMGCGSSEQGGGTGGSAGRGGAASTAGGGAS
                     GGEGAASGGVGTARVLVFSRTLGYRHDSIERGVAALDELGHERGFSVVASEDAARFAD
                     ASLGAFDVVVFLCTTGDVLDDSQQDAFRRFVQRGGGFIGIHSASDTEYSWPWYGELVG
                     AYFRAHPAIQPAALVVENGVHPATVGLPTRWLRTDEWYAFQTNPRPQVDVLLRLDESS
                     YSPGDTGMEGDHPIAWAHEFDGGRAFYTGLGHTQASYDEPEFRAHLTGAIEWAARLR"
     gene            complement(2774..3598)
                     /locus_tag="VFQ35_22705"
     CDS             complement(2774..3598)
                     /locus_tag="VFQ35_22705"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9933541.1"
                     /translation="MTNVTSRTVRLGSGHVIPQVGLGVWQMGGAETRAAVRAALELGY
                     RHIDTARIYGNEADVGRGIRDAGLAREQVFVTTKLWNAEQGYDRALKAFDASLERLGF
                     DYVDLYLLHWPVAGLRLESWRALERLHREGRARSIGVSNFLVGHLEELVGAAELTPHV
                     NQLELTPFLQRADTVAFCRKHGIVLEAYSPLTRGERLNDPVVNGIARELSRSPAQVLL
                     RWGIQQGFVVLPKSKTPERIRENSELFDFELDRAQLAKLDALEEGLTTGWDPAEQA"
     gene            complement(3658..>4110)
                     /locus_tag="VFQ35_22710"
     CDS             complement(3658..>4110)
                     /locus_tag="VFQ35_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933542.1"
                     /translation="LEYSTEVSAPNPATTLEVRIPELAPSTGTPTAMVDADATATPRT
                     LGGRRIAAIACGAVGVVGVVGGTVFGLRSMSKHDDAEPYCNGGACTDQRGVDALESAR
                     SAGNWSTASFIVGGLGLGAGALLWFWPTHENDTQLAVGLGSVKLQGRF"
BASE COUNT          613 a         1332 c         1371 g          794 t
ORIGIN      
        1 tcatcgagac cggccacgag cacagctacg agcgaaccaa gactctgacc gacatggcgc
       61 tgcaaaccgt cgacccgacg tgctcgaccc cggggaccct gtgtgtgggt ccaggtcgaa
      121 cgttctcgag cgtggtgggg ctcggaggaa acagcgtacg tgatcagagt cgctgcttgc
      181 cgagcaccta tccttacggc tgcaacggtg agtgggcgtt catctacacg aaccagcagt
      241 ccgcgactca cggcgctcag ttcatcactt tcaacgcggg cagcgcaaag acggcagtcg
      301 gatacttcaa gaacatcaac ggagctaccg tcgacacgtt cacgatccaa catgattgac
      361 gttcgaaagc ttcggggctc catgagcctc gagcggcggg ggccgagctt ggttgtgctg
      421 agcggcgtct tggctcacgc cacagcgctc ggcgcggggt tcgtatggct cgatcacgcg
      481 cacatcgagg gcggtctcgg gtgggctcgg ccgagtgagt gggcttcgct cttcactcgg
      541 ccgtttgcgg gcactggctt ctaccggccg ctgatggcgc tcgcgttgtc cctcgacatg
      601 gccgtctcgc cgacggcgcc cttcatgcac gcggtgacgc tcgcgtggca cgctctcgcc
      661 gcggctttcg tgacgtggac ggcgcgagag ctgggctcgt cgacgcgggc ctcacttctg
      721 gcgggtatgg ccttcgcggt gcatcccatg aactcgctcg tagcctccgc gatttcgttt
      781 cgctcggagg ccatgagcct ggttttcttg ctggccctcg tcttgagtca cctgcgaacg
      841 aggccggtcg cggccgcgct cgccatggcc gcatgcggat tgacgaaaga gacctcctgg
      901 ttgctgggcc cgttgttcgt caccgccctc acgctgcgct ctcggcaaga ttcgtggcgt
      961 tccacgacat ggggaaagcg tcgtctattg ttggccgagg cgttctcgtt cttggccgtg
     1021 agcttgctgc gccttcgttt cgcgccggcg tggagagccg actatcccgc cctcgcttgg
     1081 gacgagcaga ttggctcgcg gctcgccctc ctcgggaaag gcttcgcgcg cgcgctcctc
     1141 cctcttccta acacgctctg cgatgcgttt cccgtcgtgt cgctgccgag cgcgttctcg
     1201 gtgctcgggc tcgcgttgct cacgctgctg ttcgctacgc gcgcggcttc gcccgtcgcg
     1261 aaatcactct tgctcctgtc gttgctcccc tgcgtagagc tcgttccgat catgcgctgg
     1321 tggtcaccgc actactttta cgtaccgctc gcgctggcgg cgatcgcgtt ttctgacgcc
     1381 gctgaaagac gctgggggcg ctcgccttgg ctcgttctcg tcgcgctcgg agcggtggca
     1441 ttctctttgc gcgacgccct ccgctttcgc gacgacgagt cactctggtc ctacgaggtg
     1501 gcccacgaac cggcctgccg cgaagggcat ttttacttgg gcgaggtagc gcgcgagcgc
     1561 ggagacctga cacgcgccgc acggcactac cgaagcgccg ctgcgcccgt tccgggattt
     1621 ctcgcatatt cggacgagtt ttccgcatac caaaactggg gcgcggtcga gcttgcgtcc
     1681 ggccattttg ccgaagcacg tgcggcgttc gcggaggcgc gagagcgcgg gcagactcag
     1741 cggcagcgag cggaggcctc gcacaacctc gccgtggtcg cggtggcgct cggcgaggcg
     1801 cgaaccggag ccgctctttt ggcggggaca cctgagtaat ggtcggtgct gggccgagca
     1861 gcttgacgtc accggcgcgc gtgaaatgct cgaagcctcg tgacggcgaa gtcgtgtgcg
     1921 ttcgggatct tcttgctctt catgggctgc ggtagttccg aacagggtgg gggaacgggc
     1981 ggcagcgcag gccgcggagg cgcggcttcg acggctggtg gcggggcgag cggaggggag
     2041 ggcgccgcga gcggcggggt aggtactgct cgagtgttgg tgttctctcg cacgctcggt
     2101 tatcgtcacg actccatcga acgcggagtc gccgcgctcg atgaactcgg ccacgagcgc
     2161 ggtttttcgg tggttgcgag cgaggacgca gcgcggttcg cggacgcatc gctcggtgct
     2221 ttcgacgtcg tcgtgtttct gtgcacgact ggcgacgtgc tcgacgactc ccagcaagac
     2281 gcttttcggc gcttcgttca gcgcggcggc gggttcatcg ggatccactc cgcttcggat
     2341 accgaatact cgtggccttg gtacggcgag ctcgtcggcg cgtacttccg agcgcatccc
     2401 gcgatacagc ccgcggccct cgtcgtcgag aacggcgtgc atcccgcgac ggttggtctg
     2461 ccaacgcgct ggctgcgtac cgacgagtgg tacgcttttc agacgaaccc acgtccacag
     2521 gtcgacgtgc tgttgcgctt ggacgagagc tcctactctc ccggagacac cggcatggag
     2581 ggcgaccacc cgattgcgtg ggcgcacgag ttcgacggcg gccgagcgtt ttacactggg
     2641 ctcggacaca cgcaggcgag ctacgacgaa ccagagttca gggctcacct caccggggcc
     2701 atcgagtggg ctgcgcgctt acgctagcga cgtcttcatc tgccggaagc gagcggcgcg
     2761 cgacgctttc agctcaggct tgttcggcag gatcccagcc ggtggtgagc ccctcttcca
     2821 gcgcatcgag cttggcgagc tgcgcccggt ccagctcgaa gtcgaagagc tcggagtttt
     2881 cgcggatgcg ctctggcgtc ttcgatttcg gtaacacgac gaagccctgt tgaatgcccc
     2941 agcgcagcag cacttgtgcc ggcgagcgcg acagctcgcg cgcgatgccg tttacgactg
     3001 ggtcgttcag ccgctctccc cgggtgagcg ggctgtatgc ctcgagcacg atgccgtgct
     3061 tacggcagaa cgcgaccgta tcggcgcgtt gcagaaatgg cgtgagctcg agctggttca
     3121 cgtgcggcgt cagctccgca gcaccaacga gctcctccag atgacccacc aagaagttac
     3181 tgacgccgat cgagcgagcg cggccttcac ggtgcagtcg ctcgagcgct cgccacgatt
     3241 cgaggcggag ccctgcgact ggccagtgca acaggtagag gtcgacgtag tcgaagccga
     3301 ggcgctcgag gctcgcgtcg aacgccttga gggctcggtc gtagccttgc tccgcgttcc
     3361 atagcttggt cgtcacgaag acttgctcgc gagcgagccc cgcgtcgcgg atgccacggc
     3421 ccacgtctgc ttcgttcccg taaattcgtg cggtgtcgat gtgacggtag ccgagctcga
     3481 gcgccgcgcg cacggcggct ctagtttcgg ctccgcccat ttgccatacg ccgagcccga
     3541 cctggggaat gacgtgcccc gaaccaagcc gaacggtcct gctcgtaacg ttcgtcatgc
     3601 tgtagcttac gcgccgagca cgcgattgtc agggcgcgct cgggcgtgcc gagcggctca
     3661 gaaacgcccc tgaagtttca ccgagccgag ccccaccgcg agctgtgtgt cgttttcgtg
     3721 cgtaggccaa aaccagagca gtgcaccagc gccgagcccg aggcctccga cgatgaaact
     3781 cgcggtggac caatttccgg cgctgcgagc gctctcgagc gcgtcgaccc cgcgctggtc
     3841 cgtgcaggcg ccaccgttgc agtagggctc cgcatcgtcg tgcttcgaca tggacctcag
     3901 cccgaaaacg gtgccgccga cgactcctac gacgccgacc gccccgcagg cgatggctgc
     3961 gatgcggcga ccgccgagcg ttcgtggcgt cgcggttgcg tccgcgtcga ccatcgcagt
     4021 gggcgttccc gtcgatggcg cgagctccgg aatgcgtacc tcgagggtcg tggcagggtt
     4081 cggcgcgctc acttcggtcg agtactcgag
//
LOCUS       DASTKS010001078         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1957451, whole genome shotgun sequence.
ACCESSION   DASTKS010001078 DASTKS010000000
VERSION     DASTKS010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957451"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ35_22715"
     CDS             <1..353
                     /locus_tag="VFQ35_22715"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HET9933543.1"
                     /translation="LSPPLIATDRGWLMLYHGVRVTASGSLYRVGLALLAEDEPDRCI
                     ARGAPWLFGPETPYEREGDVNNVVFPCGYTVGTDGETLNIYYGCADTAIALAQTSVGA
                     LLDWIDEFGSAPDA"
     gene            complement(1456..1860)
                     /locus_tag="VFQ35_22720"
     CDS             complement(1456..1860)
                     /locus_tag="VFQ35_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933544.1"
                     /translation="MSLLGKSGDTLRRIVENGREAVRRAGERALETTSAIQATRTGSL
                     EQTGPEPPVVARLVVEIRSDGSQTIARGALEDTITGQRVAVRADGTSPAQLARSLAST
                     LVTLPLFASRIARSIQRGEIPEEVVRPKPDEK"
     gene            2065..2811
                     /locus_tag="VFQ35_22725"
     CDS             2065..2811
                     /locus_tag="VFQ35_22725"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9933545.1"
                     /translation="MSRERVLILGATSAIASEVAQIYAGRGARLHLVGRNPEKLSRVA
                     ARLSVSDATTEIADFEQLSRNEGVVDRAIATLDGLDTVLIAHGYLGDQLASERAFDEA
                     DHILRANFTSVVSLLIPLANHLEKVGGGRLAVITSVAGDRGRPRNYTYGAAKGALNVY
                     LQGVRSRLFSAGVHVTTLKLGPVDTPMTTTHKKHLLFGKPPTVAAQIVAATDAGKSEA
                     YVPAFWGAIMPIVKNTPEPLFQAMPFLSRR"
BASE COUNT          566 a         1046 c          887 g          510 t
ORIGIN      
        1 ggctctcgcc accactcatc gccaccgaca ggggctggct gatgctctat catggtgtga
       61 gggtcaccgc atcgggaagc ctttaccgag tcggcctcgc cttgctcgca gaggacgagc
      121 cagaccgttg cattgcgcgc ggcgccccct ggctcttcgg gcccgaaacc ccgtacgaac
      181 gagaaggcga cgtgaacaac gtcgtcttcc cctgcggcta cacggtcggc actgatggtg
      241 aaacgttgaa catctattat ggctgcgcag ataccgcgat cgcactggct caaacgagcg
      301 tcggagcgct gctcgattgg attgatgagt ttggttccgc ccctgacgcc tgatccgcgt
      361 gagcgtctgg acgtcgcgcg cgagcactca gtctcgctca catcttcggc gcctccgaac
      421 caacgccgcc tccaaggcgt aggtcagagc ccacaccgcc gcaccctgct gcaccgtcgg
      481 cccacgccgc tcggctccag aaatattgcg cgggatattg cgcggccgac cctgttccga
      541 acagttcggg cacgtggtcc gctcgttttc ctcggaaatt cccgtgctcg gtgccataga
      601 gggggattcg atccccctcg gctccacttc acaagtacca gtagaccttg gttagttaat
      661 agagtcgccc ttgggtcccc gcgccgtgaa gcgcgaagac cggaccaagg gacaggcaac
      721 gctggaacta accggctcgt cgatgccgcg tattcgatcg accgggatac cgcgtcgcgc
      781 gccgtgcaga gcgcgcgcaa gacggctcgt tggctcgcca gcagatgccc agcgtcactc
      841 gagacagctg ggcttcgaga ggaagactga agcgccgctc gcaacgcgcc cggctttgcc
      901 ttccacgagc tccctcgagc gccggttcgt gtcgtcgcgg cgatgcgctg tggtgcaagg
      961 cgttccgacc ccgtctttcc accgtgaggt tcactccgac cagggcgcga agcatcgcgg
     1021 attacatcgg gtccgtcgcg tcgacgtggc gatgtaatcc ttagcagggc gttgaaaaac
     1081 gcctgcgtca cgacagacca atgccgggca cacacgacgc gcagaagcaa gcatcacgaa
     1141 agcctcacat tcatgggctt tggtgatgcg cagccgcgca gtggtgccgg tcagcattct
     1201 agcagggcgt ttttcaacgc cctgttaggg cccgcgaatg accaagtcag tcgcgagcgg
     1261 gccgagaccg cagcgaggcg ggcgcgtgag gagagagcac cgcaggcctg ctgaagcggc
     1321 tgaagcaggt cgaggagcgt gacgacgagc ccgcccgccg cagcaagagg atcggcccgc
     1381 gcacagtatg acttgttcca ttcgcgggcc cttaggtagc gcgagctcgt cgtggcgagt
     1441 ttctccctgc gagcgtcact tctcgtccgg cttcggtcgc acgacctctt ccgggatttc
     1501 gccgcgttga atcgaccttg cgatgcgcga agcgaacagc ggcaacgtaa cgagcgtcga
     1561 cgctagtgag cgggcgagct gcgccgggga cgtgccgtct gccctcaccg cgacgcgctg
     1621 cccggtgatg gtgtcctcga gcgccccgcg cgcaatcgtc tgggagccgt cactccgaat
     1681 ctcgaccact agccgcgcga cgaccggcgg ctccggcccc gtttgctcga gcgaacccgt
     1741 tcgtgtcgct tgaatcgcgg acgtcgtctc gagggcgcgc tcaccggcac ggcgcacggc
     1801 ctcccgaccg ttctcgacga ttcgcctcag agtgtcaccc gacttaccga gcaagctcat
     1861 gcccatcagc ttagcgcgag cacggcggct tcgcccctcc ctccgtcgca atccccaacc
     1921 cgctccaacg atgcacacgc ggtcaaacga tgcacgcgcg gtcaaacgat gcatacgcgg
     1981 tcaaacgatg cacacgcggt caaacgatgc acacgcataa acgactcgtt cccaccgctc
     2041 caagcgcagc tatactccgc ccccatgagt cgcgaacgtg tcttgatctt gggtgccaca
     2101 tcggcgatcg cttcggaagt cgctcaaatc tacgcagggc gaggcgcgcg gcttcacctc
     2161 gtggggcgca accccgaaaa gctctcccgt gtcgcggcgc gcctctccgt ttcggacgcg
     2221 accacggaaa tcgccgattt cgagcagcta tcgcgtaacg agggagtcgt cgatcgcgcc
     2281 atcgcgaccc tcgacggcct cgacacggtg ctaatcgcgc acggctactt gggcgaccaa
     2341 ctcgcgagcg agcgcgcctt cgatgaagcc gaccacatcc tgcgagccaa ttttacgagc
     2401 gtcgtatcgc tgctcatccc cctcgcgaat cacctagaaa aagtaggcgg cggacgcctc
     2461 gcggtgatca cctcggtcgc aggcgatcgt ggtcgtccgc gcaactacac ctacggcgcg
     2521 gcgaagggcg cgttgaacgt ctacttgcaa ggcgtgcgct cccggctctt ctccgcgggc
     2581 gttcacgtca cgaccctcaa actcggcccc gtcgacacgc cgatgacgac gacccacaaa
     2641 aaacacctac tcttcggcaa gccgccgacc gtcgcggccc aaatcgtagc cgccaccgac
     2701 gccggaaaga gcgaagccta cgtcccagcg ttttggggcg ccatcatgcc tatcgtcaaa
     2761 aatacgcccg agcctctctt tcaggcgatg ccattcctct cgcggcgctg aaccgaaact
     2821 ctggcccggc gataaaccga accctggtgc gcagcgagta ctaagcccaa ctcgctcggc
     2881 gcgaccgctg aaccaaactc gcccggcgcg accgctgaac caaactcctc ggcgcgaccg
     2941 ctgaaccaaa ctcgcccggc gcgaccgctg aatcaaactc gcccggcgcg accgctgaat
     3001 caaactcgc
//
LOCUS       DASTKS010001079         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_169959, whole genome shotgun sequence.
ACCESSION   DASTKS010001079 DASTKS010000000
VERSION     DASTKS010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169959"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFQ35_22730"
     CDS             <1..664
                     /locus_tag="VFQ35_22730"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9933546.1"
                     /translation="AADLEQRLTEYEKSTGHQFALLTLDTLDGDALESFSIRVVEAWK
                     LGKKGKDDGLLLLVVKNDRKVRIDVGYGLEGTLTDALTSRVVRNVIGPAFRQGNYAAG
                     IDRAFAALMAAADEGHAAEKPAPAASGPLSWIAAVVGALFTFAPILFIVVLIIVVGRL
                     GGGGRSRTRGWGGPWGGGGFGGGGFGGGGFGGGGFGGGRGGGGFSGSGGGFGGGGASG
                     SW"
     gene            658..1284
                     /locus_tag="VFQ35_22735"
     CDS             658..1284
                     /locus_tag="VFQ35_22735"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9933547.1"
                     /translation="MVSALLSQADRERVEAAIADVERRTSLEIVVAVVARSGDYWQWR
                     VLLSICVALSAALALLEWAPRVPMLGVLFAQLPAGLLAFWASGMGWLHRLIVPKPLSL
                     ETVQAHAFRLFAQHGLHHTRDRTGLLILVSELERRVTLLGDTAVHERVGGDGWQTHLD
                     HLIARLREGRAADGILEIIARVESALGTDFPVRPDDTNELPNAVIEER"
     gene            complement(1320..2678)
                     /locus_tag="VFQ35_22740"
     CDS             complement(1320..2678)
                     /locus_tag="VFQ35_22740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933548.1"
                     /translation="MVRPEQESINLFDAEPEPAGFRPGDLIGERYQLCECIGAGAMGA
                     VFRAHDRLLDAEIAMKLVRVDSDHPDAERATQRLLLEARAIARISHPGIVRIFDFGCV
                     GGVPFIAMELLSGESLLELMLREGPLDPIRAACLLLPVADAISVGHRRAVVHRDIKPE
                     NVFLATDELGRVQPKLVDFGVARVREGGSRLTRPGALMGTPDYMAPEQAQAEPDVDHR
                     ADVWAFAVLLYELMTMRRPFSEGKQGGYLSILKAIVSDDPPNLVERGVTDQALWSIIE
                     RALEKRRERRWQSMRELGDALAEWLLDKGVEEDAYGSALRTRWAKQSISVGSMKAAEP
                     IELESLTMRKLSPPEPSDPLTMPPDATAEPPLRKSDPLRNELTLESERRLLKRDVERP
                     LEVAARIGRGNTLESTRLRNVSPIMLALLAAVIAALTTALLLVGASRFLHKPAPPSVG
                     TR"
     gene            2802..>3252
                     /locus_tag="VFQ35_22745"
     CDS             2802..>3252
                     /locus_tag="VFQ35_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933549.1"
                     /translation="MPEQTLSAISRKLAKELEELRFSAPTAYVYNPLVYARAPHEAYL
                     ERYGNGRGRVLLMGMNPGPFGMAQTGVPFGDVTMVRDYLGIEGKVKSPKELHPERPIL
                     GFECKRSEVSGTRLWGWARERFPNPRDFFERFFVVNYCPLAFVEASGK"
BASE COUNT          530 a         1072 c         1079 g          571 t
ORIGIN      
        1 ggcggccgat ctcgagcagc gcctgaccga gtacgagaag tctaccggcc accagttcgc
       61 cctgctcacc ctcgacacgc tcgacgggga cgccctcgaa tccttctcga tccgcgtggt
      121 cgaagcgtgg aagctcggga agaagggcaa agacgatggg ctcctgctct tggtcgtgaa
      181 gaacgaccgc aaggtgcgca tcgatgttgg gtacgggctc gagggcaccc ttaccgatgc
      241 gctcacctcg cgcgtcgtgc gcaatgtcat cggtcccgct ttccggcagg gcaattatgc
      301 ggctgggatc gaccgcgcct ttgcggcgct catggccgcc gcagacgaag ggcacgctgc
      361 cgaaaagcca gctcccgctg cgagcggccc actttcctgg atcgcggcgg tagttggggc
      421 gctcttcacg ttcgcgccga tcttgttcat cgtcgtcctc atcatcgtgg tcggtcgcct
      481 gggaggaggc ggcaggtcga ggacgcgagg ctggggcggt ccgtggggcg gtggtggctt
      541 tggcggtggt ggctttggcg gtggtggctt tggcggcgga ggctttggcg gcggacgcgg
      601 cggaggcggg ttcagcggaa gcgggggcgg gttcggcggc ggaggagcgt cggggtcgtg
      661 gtgagcgcgc tcttgtcaca ggcggatcgc gagcgcgtcg aggccgcgat tgctgacgtc
      721 gagcgccgca cgtcgctcga gatcgtggtc gcggtcgtgg cccgctccgg cgactactgg
      781 caatggcgcg tgctcctctc gatatgtgtc gccctcagcg ccgcgctcgc gttgctcgag
      841 tgggcaccgc gggtgccgat gctgggagta ctgttcgcgc agctacccgc tgggctattg
      901 gcgttctggg catccggcat gggctggctg caccgcctca tcgttccgaa gcccctttca
      961 ctcgagaccg tgcaagcgca cgcatttcga ttgttcgcac agcacggttt gcatcacacc
     1021 cgtgaccgga ctggtcttct cattctcgtg tcagaactgg aacggcgcgt caccctgctc
     1081 ggagatacgg ccgtgcacga gcgcgtcggg ggagacggat ggcagaccca cctcgaccac
     1141 ctcatcgcgc gccttcgtga aggtcgggct gccgacggca tcctcgagat catcgcgcgc
     1201 gtcgagtcgg cgctcggcac cgacttcccc gtgcgtccgg acgacacgaa cgagctgccg
     1261 aacgcggtga ttgaagaacg gtgagcgtcg ccgttcggcc aagagcgcgg ccgcgaagtt
     1321 cagcgcgtac cgacgctcgg cggggcgggc ttgtgcagga accgcgacgc tccgacgagc
     1381 agcagcgcgg tcgtgagcgc ggcgatcacg gccgcgagca gcgcgagcat gatgggcgac
     1441 acatttctaa gccgggtgct ttcgagcgtg ttcccgcgac cgatacgcgc cgcgacctcg
     1501 agcggccgct ccacgtcgcg cttcaagagc cggcgctcac tctcaagcgt gagctcgttg
     1561 cgcaacggat ccgacttacg aagagggggc tccgccgtgg catccggcgg catggtcagc
     1621 gggtcgctcg gttcgggcgg ggagagcttg cgcatcgtga ggctctcgag ctcgatcggc
     1681 tcggccgcct tcatcgaccc gacgctgatc gattgcttgg cccagcgcgt gcgcagcgcc
     1741 gagccgtaag cgtcttcttc cacgcccttg tcgaggagcc actctgcgag cgcgtcgccg
     1801 agctcgcgca tgctttgcca gcggcgctcg cgtcgcttct cgagagcccg ctcgatgatg
     1861 ctccacagcg cttgatccgt gactccgcgc tcgaccaaat tgggcggatc atcgctgacg
     1921 atcgctttca gaatcgacag gtacccaccc tgcttccctt ccgagaaggg gcgccgcatc
     1981 gtcatgagct cgtagagcag caccgcaaac gcccacacgt ccgcgcggtg gtcgacgtcc
     2041 ggctccgcct gcgcctgctc cggagccatg tagtcgggcg tgcccatcaa cgcgcccgga
     2101 cgcgtcagac ggctgccgcc ttcccgcacc cgcgcgacgc cgaaatcgac caacttcggc
     2161 tgaacgcggc cgagctcgtc ggtcgccaag aacacgttct ccggcttgat gtcgcgatgg
     2221 acgaccgcgc gacggtggcc gaccgaaatc gcatccgcca ccggcagcag caagcacgca
     2281 gcgcggatgg ggtcgagcgg cccttcgcgc agcatgagtt cgagcagcga ctcgccgctc
     2341 agcagctcca tcgcgatgaa cggcacccca cccacgcacc cgaagtcgaa gatacgaacg
     2401 atgcccggat ggctgatgcg cgcgatggcg cgggcctcga gcagcaatcg ctgcgtggct
     2461 cgctccgcgt cggggtgatc ggagtcgacc cgcacgagct tcatcgcgat ttcagcgtcg
     2521 agcagccgat cgtgcgcccg aaacacggcc cccatcgcgc ccgcgccgat gcactcgcaa
     2581 agctggtagc gctcgccgat caaatcacca ggtcgaaacc ctgccggttc cggctcggcg
     2641 tcgaaaaggt tgatggattc ctgctccggt cgaaccaact cttaaactcc cgccccccac
     2701 tacggccagg atgcaccgca cgaaagccgc cgtccatggt aagcccatgt gcgctcgagc
     2761 tgtcggtcgc cataccgaga catgacgaat ggtgcgtcgt aatgcctgaa caaaccctct
     2821 ctgccatcag tcgtaagctc gcgaaggagc tggaagagct gcgcttttcg gcgcccacgg
     2881 cgtacgtcta taaccccctg gtttacgcac gtgcaccgca cgaggcctac ctcgagcgtt
     2941 acggcaacgg tcgcggtcgc gtgcttttga tgggcatgaa tcccggtccg ttcggcatgg
     3001 ctcaaaccgg tgtgcccttc ggcgacgtca cgatggtgcg cgattatctc ggcatcgaag
     3061 gcaaggtgaa gtcaccgaag gagctgcacc cagagcgtcc gatcttgggc ttcgagtgca
     3121 agcgaagcga ggtgagcggc acacgcctgt ggggatgggc acgtgagcgc tttcccaacc
     3181 cgcgcgactt cttcgaacgc ttcttcgtcg tcaactattg tcccctcgct ttcgtcgagg
     3241 cgagcgggaa aa
//
LOCUS       DASTKS010001080         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_391954, whole genome shotgun sequence.
ACCESSION   DASTKS010001080 DASTKS010000000
VERSION     DASTKS010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391954"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFQ35_22750"
     CDS             complement(<1..567)
                     /locus_tag="VFQ35_22750"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9933550.1"
                     /translation="MQHRVLSVGSGLAHADFRGAAQHSFDVQPCATLSLALDRAARAS
                     FECILVSSHLPDAPGTEAVQRLRRECGDTPLLFVSDTPDVDFREWGADEVFTSHEVGT
                     RLFARSLLYAIEQRRVRVQHRQLERLLETIPDAILVTNRRGKVRYVNPAALELFGCAR
                     LDLEEELLAFAAKDREPFEINVLRKGDIR"
     gene            complement(628..921)
                     /gene="kaiB"
                     /locus_tag="VFQ35_22755"
     CDS             complement(628..921)
                     /gene="kaiB"
                     /locus_tag="VFQ35_22755"
                     /inference="COORDINATES: protein motif:HMM:NF006798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HET9933551.1"
                     /translation="MTKFKLRLYITGRTAQSQRTIENLRLLCESELRGHYEVDVIDVL
                     EHPVLAENEKILATPTLVKQLPEPVRKIIGDLSDRERVLIGLDLEQVAKADEA"
     gene            complement(1185..1598)
                     /locus_tag="VFQ35_22760"
     CDS             complement(1185..1598)
                     /locus_tag="VFQ35_22760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9933552.1"
                     /translation="MSRGSAALRVLVADDARAVAERIAGLVLDSGSQVSLIGPAFDGS
                     EALTLFERERPDLALIDLHMPGLDGLQLTRSIRKADEHCRVVVLTSHLEPVVREECLA
                     AGAECVLDKVRELGRLELLLREEVERRSRASGEPV"
     gene            complement(1714..>2656)
                     /locus_tag="VFQ35_22765"
     CDS             complement(1714..>2656)
                     /locus_tag="VFQ35_22765"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9933553.1"
                     /translation="VLPNTQPAAAVQVGERILRELAGCPLPPELQSRRLTVSIGVSVI
                     SGLSSTLEDIIAAASGALKNAKGRGKHRVWLSDEQSGYGLNASPEALTSVSFRDLRIV
                     TQPIRDLRDGTLKGRELFVRGPNTELEKPRELFEFARNLGILERLDLECLRASLKACS
                     TFGGPRLHVNVLPSTLTRYEALLELLREQSLPTKLCLEVDEREIVGDPGKLRPARAYL
                     KEHGILLGLDNVGFGRNALETIVVLKPDIVKVSVDLLANVARDRGQVRDLRRLVAFAE
                     SAGCEIIAQGVESSRDVGTLLETGIRSAQGFLWDRPD"
BASE COUNT          461 a          853 c          842 g          500 t
ORIGIN      
        1 gcgtatgtcg cctttgcgaa gcacgttgat ctcgaaaggt tcgcggtcct tggccgcgaa
       61 agccagcagc tcttcctcga ggtcgaggcg cgcacagccg aatagctcga gggcggccgg
      121 gttgacgtag cgaactttgc cgcgacggtt cgtcaccaaa atggcgtcgg ggatggtctc
      181 gagcaaacgc tcgagttggc ggtgctgcac gcgcacgcga cgttgctcga tcgcgtacag
      241 caagcttcgt gcaaatagcc tggtacccac ctcgtgcgac gtgaagactt cgtccgcacc
      301 ccactctctg aagtccacgt ccggcgtgtc agaaacgaag aggagcggtg tgtccccgca
      361 ctcccgacga agacgctgaa ccgcttccgt cccgggggcg tcgggcaagt gagagctgac
      421 cagaatgcac tcgaacgacg cgcgcgctgc ccgatccagg gccagcgaga gggtcgcgca
      481 cggctgcacg tcgaagctgt gctgggcggc cccgcggaag tcagcgtgag ccaggccgct
      541 gccaaccgat agtacccggt gttgcacgat gccatctccc gaattcagga tcgatcaacc
      601 tctgccatgt tccgaattgc gagtgactca agcttcgtcg gctttcgcca cctgctccag
      661 atcgagcccg atcagcacgc gctctcgatc actcaggtcc ccgatgatct tccgcactgg
      721 ctcgggaagc tgcttcacga gcgttggcgt cgcgaggatc ttctcgtttt ctgcgagcac
      781 ggggtgctcg agcacgtcga ttacgtcgac ttcgtagtgc cctcgaagct cggactcaca
      841 aagtaggcgc aagttttcga tcgtgcgctg cgattgcgcg gtgcgcccgg tgatatagag
      901 tcgcaacttg aacttggtca tgatggtgtg ctttctcgtg cgccgctcag gggcgttccg
      961 cggcatcgct attctcggcg cacctctgct cgtcggcgct cccgagcggg agggccagtc
     1021 atgctcttca cgttcatggc tccaatatcg acacgccggg gaggcgcgcg tagtcgcaag
     1081 attgctgagg tcgtgtcagc gagttcccga cagccgacgc tcgttgagag tcgtggcgcg
     1141 gcgtgcctcg ggcccggacc gcagcgccgc cgctcgcgcg gaggttagac cggttcgccg
     1201 ctcgcccggc tccgacgttc gacctcttcc ctgagtagaa gctcgaggcg tcccagctcg
     1261 cggaccttat ccagcacgca ctcggcgccg gccgcaaggc actcttcccg aacgacgggc
     1321 tccaagtggc tcgtaagaac cacgacgcga cagtgctcgt cggctttgcg aatggaacga
     1381 gtgagctgga gtccgtcgag accgggcatg tgtaggtcga tcagggccag gtcgggccgc
     1441 tctcgttcga aaagcgtcag cgcttcgctc ccgtcgaagg ccgggccgat cagcgatacc
     1501 tgcgaaccgg agtccagaac tagccccgca atgcgctccg cgacggcccg cgcgtcgtcg
     1561 gcgaccagga cacgtagcgc ggcggagcct cgactcactc acccaatcta gggcccgcca
     1621 cctgatcgct ccatcggtgc gtttcgcaat gtagtgtcag gaaattactg atccccttgg
     1681 tgcggcgggg ttcgaaacgg tgatgaagcg ctctcaatct gggcggtccc agaggaagcc
     1741 ctgggcgctc cgaatccctg tttcgagcag ggttcccacg tcgcgcgaac tctcgacgcc
     1801 ctgggcgatg atctcacagc cggcgctttc ggcgaacgca acgagccgcc gcaagtcgcg
     1861 gacttggccg cgatctcgtg cgacattcgc cagcaagtcg acgctgacct tcacgatgtc
     1921 gggcttcaag accacgatgg tctcgagcgc gtttcggccg aacccgacgt tgtccaagcc
     1981 cagcaggatt ccgtgctctt tcaggtacgc tcgtgcgggg cggagttttc cagggtctcc
     2041 gacaatctct cgctcgtcca cctccaagca gagcttggtc ggtagcgact gttcccgcag
     2101 gagttcgagg agcgcctcgt atcgcgtcaa ggtgctgggg aggacgttca cgtgcagcct
     2161 cggtccaccg aacgtcgagc acgccttcag gctcgcgcgc aggcactcga gatccaaacg
     2221 ctccaggatc ccgaggttcc gagcgaactc gaacagctcg cgcggcttct cgagttcggt
     2281 gttcgggccg cgaacgaaca gctcgcgacc tttcagcgtg ccgtcgcgca agtctcggat
     2341 cggctgggtg actatgcgga gatcgcgaaa tgagacgctc gttagagcct ccggcgaggc
     2401 gttgagcccg tagccggact gctcgtcgct gagccagacc cgatgcttgc ctcgaccctt
     2461 cgcgttcttc aatgcacccg aggcggcagc gattatgtct tcgagggtgc tcgagaggcc
     2521 agagatgacc gagacgccga tgctcacggt caaccgtcgg ctttgcagct cgggtgggag
     2581 agggcaaccg gcgagttcgc gcaagattcg ctcgcccact tggaccgccg cggcgggttg
     2641 tgtgttcggc aacacc
//
LOCUS       DASTKS010001081         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1511197, whole genome shotgun sequence.
ACCESSION   DASTKS010001081 DASTKS010000000
VERSION     DASTKS010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511197"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFQ35_22770"
     CDS             <1..608
                     /locus_tag="VFQ35_22770"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9933554.1"
                     /translation="RALQERMLRPVGGEKEVPWKARVVAATNRDLDTLVARGEFREDL
                     YYRLNVIRVDVPPLRQRREDIALCAQFFLQSFAQSLGKSVVGFSPAALDDMMAYSWPG
                     NLRELQNCVHRAVALSNFDHVRVQDLPERVRRPASAEPATPAIEIARESLAEHERRYI
                     LKVLSDAHGNKAEAARILGIERRTLYRKLAEYELAGARDAE"
     gene            821..1225
                     /locus_tag="VFQ35_22775"
     CDS             821..1225
                     /locus_tag="VFQ35_22775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933555.1"
                     /translation="MATDRILLVDDDPDIRTIASMALRAWGKFEVVSAESGEAALEMA
                     LSANHDLLLMDVMMPRLDGIGVASELRARGVIPNVPLIFLTAKAEPRDIELYLDLGAI
                     GVIVKPFEPGHLAREARRLLGICRSVRLSVRR"
     gene            1366..1785
                     /locus_tag="VFQ35_22780"
     CDS             1366..1785
                     /locus_tag="VFQ35_22780"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9933556.1"
                     /translation="MGAQLRSPSRKIAPAILPTDADDFEEQLRLIRREYACKLPGKVS
                     ALAACLAEREKNLRVLAEARELAHRLKGTAGTYGFNGVAELAARLERSLSALVDDRAG
                     GATLILKEIDELLAELRALAELEFENELASDPTNRPR"
     gene            1886..2509
                     /locus_tag="VFQ35_22785"
     CDS             1886..2509
                     /locus_tag="VFQ35_22785"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET9933557.1"
                     /translation="MPKPTRKSPDIERSEAQIAGAAEAYPNVIETGPWGHRAFKIGGK
                     TFLFLSADGEGLSFSVKLPITGKAALGLRFAEPTHYGLGKSGWVTARFAPGEDIPLPK
                     VLEWVDESFRAIAPKKVVNALAPSLAQPSGGAKKSSPKKVAAEKLPPKKAAAKEAPAK
                     RVAAPRKSAAKDALAKRSVAAKKDSAKNAPLKKTGANKARTKRSKPT"
BASE COUNT          507 a          819 c          827 g          458 t
ORIGIN      
        1 tccgcgcgct gcaagaaaga atgcttcgcc cggttggggg tgagaaagag gttccttgga
       61 aggcgcgcgt cgtcgccgcg acgaaccgag acctcgatac tctcgtggcg cggggtgagt
      121 ttcgcgagga cctctactac cggctcaacg tcattcgcgt tgacgttccg cctctgaggc
      181 agcgacgtga agacatcgcg ctctgtgctc aattcttctt gcagagcttc gcgcaaagcc
      241 taggcaagag cgtcgtcggc ttctctcccg cggcactcga cgacatgatg gcgtattcgt
      301 ggcccggtaa tctgcgtgag ctccaaaact gcgttcaccg cgcggtggcg ctctccaact
      361 tcgatcacgt tcgcgtgcaa gacttgccgg agagggttcg ccgtccggct tcggccgaac
      421 cggcgacccc cgcgatcgaa atcgcgcgcg aatcgctcgc ggagcacgag cggcgctaca
      481 tcttgaaggt cctgtccgat gcccacggca acaaggccga ggcagcgcgc attctcggga
      541 tcgaacgtcg taccttgtac cgcaagctcg cagagtatga gctcgcgggc gcccgcgacg
      601 cagagtaagt ctctcggcta cgcagagggc tctcgggacg tgtcgctcgc gagtcatttc
      661 gacacaccaa ttcgcgcgag gttcacgcga gtgagtcgtt tcgacacagc ctgagtcgcg
      721 gcgagcagct ttttcgcaat ctccggcgtt ctcgtcgaat ggtgtgctcg ttgcatcgat
      781 tccttcgccc gcgccgacct tttcgtgcgc agcgcacacc atggcaaccg acagaatact
      841 cctcgtcgat gatgaccccg acattcgcac catcgccagc atggcgctcc gggcgtgggg
      901 caagttcgaa gtggttagtg cggagagtgg cgaggcggcg ctggagatgg cgctctccgc
      961 gaaccacgat ctcctgttga tggatgtcat gatgccgcgg ctcgacggca tcggcgtcgc
     1021 ctcggaactt cgcgctcgcg gcgtcattcc gaacgtgccg ctgatctttc tgacggcgaa
     1081 ggcggagccg cgagacatcg agctctacct ggacctcggt gcgatcgggg tcatcgtcaa
     1141 gccgttcgaa ccaggacact tggcgcgaga agctaggcgg ctcttgggta tttgtcggag
     1201 cgtaaggcta tcggtgcgtc gctgagctac ttcgtctccg attggccttc ggattggccc
     1261 agggcaagcg actggcctgg cgcgcaacga tctgccgcgc gaaggattcg gcgcggcggc
     1321 ggcggtcgca agtggcacga tctgttaggg cgctcgtcga gcgaaatggg cgcgcagttg
     1381 cgctcgccgt ctcggaagat cgctcccgcc atcttgccaa ccgacgcaga cgacttcgaa
     1441 gagcagctgc gcctgatccg gcgtgaatac gcctgcaagt tgccgggcaa agtctcggcg
     1501 ctggcggcgt gtctggccga acgagagaag aacctgcgcg tactcgccga ggcgcgcgag
     1561 ctcgcgcacc gcctgaaagg aacggcgggc acctacggtt tcaatggagt ggctgagctc
     1621 gcggctcgct tggaacgcag cctgagcgcg ctcgtcgacg accgtgccgg gggggcaacc
     1681 ctgattttaa aagagatcga cgaactgctc gccgagttgc gcgcgctggc cgagctcgag
     1741 ttcgaaaacg agctcgcgtc cgaccccacg aaccgacccc gctgaactcc gcaaccgcaa
     1801 agggcgcaac cgccacacta cgccgatgtc ttcaccacca gcccgagacg gttgtggttc
     1861 gagcgcaatt ggtgaaacat ggcgcatgcc gaaaccaacg cggaagtcgc ctgacatcga
     1921 gcgctcagaa gcgcaaatcg cgggcgctgc cgaagcctat ccaaacgtca tcgaaacggg
     1981 tccgtggggc caccgcgcct tcaagattgg cggaaagaca tttctgtttc tctccgcgga
     2041 cggagaaggt ctgtcatttt cggtcaagct gcccatcact ggcaaagccg cgctcggctt
     2101 gcgctttgca gagcccaccc attacggact cggcaaatcg gggtgggtga cggcgcgctt
     2161 tgcgcccggc gaagacatcc ctttgcccaa agtgctcgag tgggtcgacg agagctttcg
     2221 cgcgatcgct cccaagaagg tcgtgaacgc gctcgcgccc tcgctggcgc agccgtcggg
     2281 cggcgcgaag aaatcttccc cgaaaaaggt cgctgcggag aagcttcccc ccaagaaggc
     2341 ggcggccaag gaagctcctg cgaaaagggt cgctgcccca cggaaatccg ccgccaagga
     2401 tgctctcgcg aaaaggagcg tggccgcgaa aaaagactcc gcgaagaatg cgcctctgaa
     2461 aaagaccggc gccaacaagg cgcgcaccaa gcgctcgaag ccgacctagc agggcgttga
     2521 aaacgctctg cgtcacgaca gaccaatgcc aggcacacac catgcgcagc cggacggtgg
     2581 tgccagtcag cattcaggca ggcgttttca a
//
LOCUS       DASTKS010001082         9649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_170279, whole genome shotgun sequence.
ACCESSION   DASTKS010001082 DASTKS010000000
VERSION     DASTKS010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170279"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1628
                     /locus_tag="VFQ35_22790"
     CDS             63..1628
                     /locus_tag="VFQ35_22790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933558.1"
                     /translation="MLLAVAIASLAVLFGLLLGLAPAASRRALGPLRTLALTAVIGVV
                     ALHLLPEALRDLGAVGLLVFAIGLALPRWLNALRAKRPHAHGEHEHARGDELGLDLGF
                     FGLLAHHVGDGLALGAYSRAGATHGHPHGDVLLALVLHTVPLVAVVAAGYARTRGVRV
                     AVTRAAWLAVASIVGVLCSHLVPESIVDRTTAWIAAGVSGLLLHGLTHDLELDLPRAP
                     AAKSFDLAMALLGAAVGILGAKLGAHADRAAEARLRLALLTELDRVAVPLACGLALGA
                     LLALGRSPKVVSLLGFYRRASRPERGGVLTPEAFLLTLSHFGFPIAVLRDAVSGIGFA
                     LRGASPEEALEDATIGASSAGSASAESAVTSAVPSAFARVDTIVPWAALGVLVSAVIR
                     ASVPDFALASSLWLAIPVAILLTLSVKVHAVAAPSLAYALADRGLPMPAAIVALVLLP
                     FALDLRNGKQALFLLIPVAFAFALGDRLPHAPLVIDSRIALVAASGIGALLLARSYEL
                     GFRGVLLTILKFD"
     gene            complement(1653..2588)
                     /locus_tag="VFQ35_22795"
     CDS             complement(1653..2588)
                     /locus_tag="VFQ35_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933559.1"
                     /translation="MSSIRLPCRALILGAGLLTTRALAQPTQKLDAQPGAEEVLKDAV
                     SQVVTQSPPTPPADFGYWSRGAARWFLSTRSELGTPYAKPYFSAGYGQPHWLWTGVDV
                     NAIVTPEFMQGYFGLRAASPVLDLAFGWRDTKSFTKPFLQPQTSYDREDVLDARNSRA
                     RYWAWEAEAVAILPLPHAALVADLVAVHTIDVPEGRLVYDESYRAVIKNATFGVIRAV
                     AVARILKEDSLKLGLLGEVVFGTGRDKPVTRVGPVVALQITDHLEALGGVSLATSSPD
                     QLGLSLGAYGVCGLRYRWATNEAEPKLPWSGQVIP"
     gene            2882..4483
                     /locus_tag="VFQ35_22800"
     CDS             2882..4483
                     /locus_tag="VFQ35_22800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933560.1"
                     /translation="MADGGATSAGGRGSFGGASLGGAGSTSKGGRSSSGGQPAQGGQG
                     LGGAPLGGGTSTGGRQTSGGAPAQGGAPARGGAPASGGSPASGGTPSFGGAPAFGGAP
                     AGGSGGGGGCPPCKAYSLTACSYEVCDSATRKCVPVSREGQSCDDGNFCTLNEVCRGG
                     FCQGGVKNTCGAVESECLQVTCMPQGYCNRVPTEGKACKTGSGNLCEIDARCVASKCQ
                     PTYRDCGGIAPMDPCFSGQCNKSTGQCEAIPLSGNACTPSDLCSASGVCVTGKCVSQS
                     SVDCSGFDTLCTWGQCEPSSGLCSSRNQPNGTPCDGNDACVRGSTCANGVCAGGMRIT
                     SCSSGDGCCPSGCTGKTDNDCSATYVLDAVNRGWWNSLGEHVSSNDNTFTGKLNGSTY
                     NSYFTFDLSGLSADISAVRLRLELESVLGNDTSEQISLWDVSTDATTLEADASGRTPV
                     IFQDLQSGLIYAMTNAFRSAVGTTLTISLGPAAVANINAARGGYFSVGIHVDTANGAG
                     TSGGEGVRFSEASEFRIHQLELDRL"
     gene            complement(4593..6392)
                     /gene="sppA"
                     /locus_tag="VFQ35_22805"
     CDS             complement(4593..6392)
                     /gene="sppA"
                     /locus_tag="VFQ35_22805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9933561.1"
                     /translation="MKRVIALAALALAVGCKGRPRVNENKDEAREPGEKEARVVAVDL
                     GSGMPEASRGGLIQMPATRTYTGLVRALERAATDPLTASVYVRLGAQGIDFSRAEELG
                     QLFERFHKNGKNVVCHAHAYTNATSWLAVRGCSRIWLSAAGSVDTVGIAAQLVHLKGL
                     LNRLKVDADILHVGKYKSGGEPLTREEPSEYARTSLQSTLGSMREAWLKSAELGRPGH
                     ELGKKLEAGPYSPEEAKSLGIVDAVGFESDALEEAKKLGHTRAVAHVFGPKSKKDDGL
                     DIGALIRVLTGAEDNGGGKPHIAVVPAEGAIGMEAGGPLDGPGITAQALTRTLKKLAA
                     DDSVKAVVLRIDSPGGSPLASDLIWHEMMELRKKKPIVASVGSMAASGGYYIACGAQR
                     IVAEHTSIVGSIGVFGGKVVIGPALHEIGVNTFTVPASTEPGAEARATYLSPFTPWDD
                     ATRTRVQAQMQSIYDLFVARVAEARKMPVERVREHAEGRIWSGDQGKERGLVDDFGGL
                     STALEIARKLAKLEPSTPVTVEGSKDGLLDLLGLDNDASQAALESAILRFEREHALLA
                     SLPSGLRAQASALSPLAHGENVVAALPYALDLH"
     gene            complement(6389..8620)
                     /locus_tag="VFQ35_22810"
     CDS             complement(6389..8620)
                     /locus_tag="VFQ35_22810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9933562.1"
                     /translation="MDVRCNRCATEYEFDDALISERGTTVKCTNCGYQFKVFPTGSQS
                     QEPERWVVRTANGDELVYTSLRELQRGIADQKVGPNDLLSRGRQPPRALGAIAELEPF
                     FHESAQKSARPARTLAGVAPPHPVLANASAIPPRASAEPRGPVAHEAPEDSRRHTQPG
                     LGEASSASFEAEREELPESSLSAAPTPALPPVEPVSVRANSAAPSSTSLPVSEMQPVA
                     SQNAPLSGREYMLTPSSPFAAAPIPPELMPVPSSPYSSGPISAGRRDLRSYDELTHEE
                     IHEHGRRARSRWIAAFVIAGVTTLFALTVGRRYLLENAGSSSQEAAASNARVATLLRD
                     GNRLLGEGDVDGASEQLLRASALAEKDHAVLAALAHLETIRADISWLKLRLLDPELGD
                     LVQATHRELGRRVGRARLAADAAFAVAPEDIVVLRARVDTLRLSGEADKAREWIKPIA
                     ANPSDPQNAYVLAALDLSDPNPGWGSIVDRLRVAVSGERNAGRAHAALIYALARGGKL
                     TEAETEFGKLESSASTPLLQDELRGFLKRYSAGKDASPSAPVAMVDPGKLGKLDTSQA
                     EEPKAAAKGATTPNVEAARASSGDFRKQLADASKALSKGSLSEAESLYERVLTAQPNA
                     TEALAGLADIARRRHDPATAARLYDRVLANNPNYLPALMARGDQEWDSGNRAAAISFY
                     KRVVEQSGSGSSYGQRASARIAEQGGSTTEKPAEKAPPPPASQEKPSDTPEVDTSDLP
                     GFK"
     gene            8879..9334
                     /locus_tag="VFQ35_22815"
     CDS             8879..9334
                     /locus_tag="VFQ35_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933563.1"
                     /translation="MKRFAVVVLLLAVGSILPGCPIYDDDSGCSVDEDCPVDYACDSL
                     VGSCRPNACSAPTQCPADQTCSRGGKCVALDCSWPEVGCVAGYTCSSRTKTWECVKAS
                     TGGEGGNGGAGATATGGTGESSGGLSASGGASPDATGGTPADTSGGTSG"
     gene            complement(9383..>9649)
                     /locus_tag="VFQ35_22820"
     CDS             complement(9383..>9649)
                     /locus_tag="VFQ35_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933564.1"
                     /translation="APEPEAPATTAEGEGEVTKGKKKRARKAKRTDSAEKEPKAAKAP
                     RAPAREAVARPRPEPALASASQESSASQPPKSRRELPPYLRVIK"
BASE COUNT         1384 a         3173 c         3328 g         1764 t
ORIGIN      
        1 ttttcccgcc gaactcgcac cgggcgtgct gaagcgcggc agatccgcta aaacgcccgc
       61 ccttgttgct tgccgtagcc atcgcctcgc tggccgtgct gttcgggcta ttgctcgggc
      121 tcgcgccagc cgccagccgc cgcgccctcg ggccgctgcg gacgctcgcg ctgacggcgg
      181 tcatcggcgt ggtcgcgctg catctcttgc cggaggcctt gcgcgacttg ggcgcggtcg
      241 ggctcttggt gtttgcgatt ggtctcgctt tgccgcgctg gctcaatgcc ctccgcgcca
      301 agcgccctca tgcccacggc gagcacgaac acgcacgcgg tgacgagctc gggctcgatc
      361 taggtttttt cgggctgctc gcgcatcacg tcggagacgg ccttgccctc ggcgcgtatt
      421 cgcgagccgg cgcgacacac ggccatcctc acggcgatgt cttgttggcc ctcgtgctcc
      481 acaccgtgcc tctcgtggcg gtcgtggccg ctggctacgc gcgaactcgg ggcgtgcgag
      541 tcgcggtgac gcgcgcggcc tggctcgcgg ttgcaagcat cgtgggtgtg ctgtgctccc
      601 atttggtacc cgagagcatc gtcgaccgga ctacggcgtg gattgccgcg ggtgtttcgg
      661 gcctgctgct ccacgggttg acgcacgatt tggagctcga tttgccgcga gcgcctgcgg
      721 cgaagtcgtt cgacctcgcc atggcgctgc tcggcgctgc ggtgggtatc ctcggggcga
      781 agctcggcgc tcacgccgat cgcgcggccg aggcgcgttt gcgcttggcg cttttgaccg
      841 agctcgatcg agtcgcggtg ccgctcgcgt gcggcctcgc tctcggcgcg ttgctcgctc
      901 tcggtcggag cccgaaggtc gtctcgttgc tgggcttcta tcgtcgggcg tcgcgtcccg
      961 agcgaggcgg cgtgctcacg cccgaagcgt tcttacttac gctgtcgcac ttcggtttcc
     1021 ccatcgccgt gttgcgagac gccgtgtcgg gcatcggctt tgcgctccgt ggcgcttcgc
     1081 ccgaggaggc gctcgaggat gcgacgatcg gagcgtcctc ggcagggagc gcctcggctg
     1141 aatccgcggt gacgtcggcc gtgccctccg cttttgcgcg ggtggacacg atcgttccgt
     1201 gggcggcact cggggtcttg gtctccgcgg tgatccgagc ttcggttccc gactttgcgt
     1261 tggcgtcgtc actgtggcta gcgatcccgg tcgccatcct cctcaccctc agcgtgaagg
     1321 ttcacgccgt cgccgcgccg tcgctcgcct atgcactcgc cgatcgcggc ctaccgatgc
     1381 cggccgcaat cgtcgcgctc gtgctgctgc cgttcgcgct ggacttgcgc aacggaaagc
     1441 aagcgctgtt cttgctgatt ccggtggcct tcgcgtttgc actcggcgat cgtctgccgc
     1501 acgcgccgct cgtcatcgac tcgcgcatcg ctctcgtcgc ggcctccggc atcggtgcgc
     1561 tgctcctcgc acgctcgtac gagctcggct ttcgcggcgt ccttttgacg atcctcaagt
     1621 tcgattagcg agaccgcgag ctgggggcgc ggctagggaa tcacttggcc cgaccatgga
     1681 agcttgggct ccgcttcgtt cgttgcccaa cgatagcgaa gaccgcacac gccgtacgcg
     1741 ccgaggctca acccgagttg atccggactc gaggtcgcga gcgaaacgcc gccgagtgcc
     1801 tccaagtgat ccgtaatttg cagtgcgacg acgggaccta cgcgagtgac cggcttgtct
     1861 ctgcccgtgc cgaagacgac ctcgccgagc agccctaact tgagcgagtc ctccttcagg
     1921 atgcgcgcga ccgcgaccgc gcgaatcact ccgaaggtcg cgttcttgat cacggcgcga
     1981 tacgactcgt cgtacacgag ccggccttcg gggacgtcga tggtgtgaac ggcgacgaga
     2041 tcagccacga gcgccgcgtg agggaggggc agtatcgcga ccgcttcggc ttcccacgcc
     2101 cagtaacgag cgcgactgtt gcgggcgtcg agcacgtcct cgcgatcgta gctcgtttgc
     2161 ggttgtagga acggcttggt gaacgacttc gtgtcgcgcc agccgaacgc caagtccagc
     2221 acgggagagg cagcgcgcag gccgaagtac ccctgcatga actcgggcgt cacgatcgcg
     2281 ttgacgtcga caccggtcca gagccagtgc ggttgcccgt agccagcgga gaagtacggc
     2341 ttggcgtacg gagtaccgag ctcgctccga gtcgacaaga accaacgagc tgcgccgcga
     2401 ctccagtacc cgaagtccgc tggcggcgtt gggggtgatt gtgtcaccac ctgcgacacc
     2461 gcgtctttga gcacctcctc cgcgccgggc tgcgcgtcga gcttctgcgt cggttgcgcc
     2521 agggcgcgcg tcgtcagcag ccccgcaccc agaatgagcg cgcggcacgg taagcgaatg
     2581 gaactcatgg acaagcgccg cctcgaggca cggcgttggc gcccgtacca cctggtcgta
     2641 ccaataggaa gcggtcaagg gcgcgcgttg cgaggtctcc agcaatttac aacgacgtcg
     2701 cgtcgttttc tcccagcgtc cgccgtccgc ccgccttgag gccagaaagg cgctctcttt
     2761 ttaggcgctg tggcgtgttg gccgctatgc ttggggtgac atgtcgggcg gtcgtttcgc
     2821 gggtcctttg acggcgcttc ttctttttgc gggagcgtgt ggcgggcgta gcgaaggagg
     2881 tttggccgac ggcggtgcta ccagcgcagg tggtcgaggg tcgttcggcg gagcctcgct
     2941 cggcggcgct ggctcgacct cgaagggcgg acgttcgagc tcgggcgggc aacctgcgca
     3001 aggcggacaa ggcttgggcg gtgctccgct cggcggcggg acaagcacgg gcggcagaca
     3061 gacgtctgga ggggctcccg cgcaaggagg cgctcccgcg cgaggaggcg ctcccgcgtc
     3121 gggaggaagc cccgcgtcgg gaggcactcc gagtttcgga ggggctccgg cgtttggtgg
     3181 cgctcccgcg ggtgggtccg gtggtggagg cggctgtccg ccgtgcaaag cctattcact
     3241 caccgcttgc tcgtacgagg tctgcgactc ggcgacgcgc aaatgtgtcc cggtttcgcg
     3301 agagggacag agctgcgacg acgggaactt ctgcacgctg aacgaggtct gccgcggcgg
     3361 gttctgtcaa ggtggggtca agaacacgtg cggcgccgtg gagagcgagt gccttcaagt
     3421 cacgtgcatg ccccagggct actgcaacag agtcccgacc gagggcaaag cgtgcaagac
     3481 gggcagcggc aacctttgtg agatcgacgc gcgctgtgtc gccagcaagt gccagccgac
     3541 atatcgagac tgcggcggca tcgcccccat ggatccttgc ttttcggggc aatgcaacaa
     3601 gtctacgggt caatgcgagg cgatcccgct cagcggaaac gcttgcaccc cgagtgacct
     3661 ttgcagcgcg agcggcgtgt gcgtgaccgg caagtgcgtg tctcagagct cggtcgactg
     3721 cagcggcttc gacacgctgt gcacctgggg acagtgcgag ccgagcagcg ggctctgctc
     3781 ttcgcggaac caaccgaacg gtaccccgtg tgacggcaac gacgcctgcg tgcgcggcag
     3841 cacgtgcgcg aacggcgttt gcgctggggg tatgcgcatc acgagctgct cgtcgggtga
     3901 cgggtgctgt cccagcggtt gcacgggaaa gaccgataac gactgctccg ctacgtacgt
     3961 gctcgacgcc gtgaaccgcg gctggtggaa ctccctcggc gagcatgtct ccagcaacga
     4021 caacaccttc accggaaagc taaacggcag cacctacaac tcgtacttca cgttcgactt
     4081 gagcggcctc tccgcggaca tcagcgcggt gcgcctgcga ttggagctag aaagcgtcct
     4141 cggcaacgac acgtccgagc aaatctcgct gtgggacgtc agcaccgacg cgacgacgct
     4201 cgaggccgac gcgagcggtc gcactccggt gatttttcaa gatctgcaat ccggattgat
     4261 ctacgcgatg accaacgcct tccggagcgc cgtcggcacg acgctgacga tttcgctcgg
     4321 gcccgcagcg gtggccaaca tcaacgcggc tcgcggcggc tacttttcgg tggggattca
     4381 cgtcgacacc gcgaacggcg cagggacgtc gggcggagag ggtgtgcgct tcagcgaggc
     4441 cagcgagttt cgcatccatc agctcgagct cgaccggctc tgagcaatcg cttcgacgcg
     4501 cagcgtcgat gcatcgatgc gagccggctc tgagcaatcg cgtcgatgcg cgccgtcgag
     4561 agcgcggcag cgcgggcgcg gcagcgcggg gttcagtgaa ggtcgagcgc gtaggggagg
     4621 gctgcgacga cgttctcgcc gtgcgcgagc ggagacagcg cgcttgcctg ggcgcgcaac
     4681 cccgacggta gcgaagcgag caaagcgtgc tctcgctcga agcgtaaaat cgccgattcg
     4741 agcgcggcct ggctcgcgtc gttgtcgagg cccaacagat cgagcaagcc gtctttggag
     4801 ccttcgaccg tcaccggcgt gctcggttcg agcttcgcga gcttacgagc gatctcgagc
     4861 gcggtcgaaa gccccccgaa gtcgtcgacg aggccgcgct ccttgccttg atcgccgctc
     4921 cagatccgtc cctcggcgtg ctcacgcacg cgctccacgg gcatcttcct ggcttccgcc
     4981 acgcgcgcta cgaacaggtc gtagatgctc tgcatttgag cctgtacccg ggttcgcgta
     5041 gcgtcgtccc agggtgtgaa cggcgaaagg taagtggcgc gcgcttcggc gcccggctcg
     5101 gtgctggccg gcaccgtgaa cgtattgacc ccgatttcgt ggagcgcggg cccaatgacg
     5161 accttgccac cgaacacgcc gatcgacccg acgatgctcg tgtgttcggc gacgatgcgc
     5221 tgcgcaccgc aagcgatgta gtagccgccg ctcgcggcca tgctgcccac cgaggccacg
     5281 atgggcttct tctttcggag ctccatcatc tcgtgccaga tgaggtcgct cgcgagcggc
     5341 gagccgccgg gagagtcgat gcgcaacacg accgccttca ccgagtcgtc cgcggcgagc
     5401 ttcttcaaag ttcgtgtcag cgcttgggcg gtgatgccgg gaccatcgag aggaccgccg
     5461 gcctccattc cgatcgcgcc ttcggcgggc acgacggcga tgtggggctt acccccgccg
     5521 ttgtcctcgg cgccggtgag cacgcggatc agcgcgccga tgtcgaggcc gtcgtctttc
     5581 ttcgacttcg ggccgaagac gtgcgccacc gcccgcgtgt ggcctagctt cttggcctct
     5641 tccagcgcgt ccgactcgaa gccgaccgcg tcgacgatgc cgagcgactt ggcctcttcc
     5701 ggtgagtaag ggccggcctc gagcttcttc ccgagctcgt gcccgggacg acccagctcg
     5761 gccgatttga gccaggcttc acgcatcgag ccgagcgtcg actgaagcga cgtgcgggca
     5821 tattcgctcg gctcctcccg cgtgagtggc tcgccgccgc tcttgtattt tccgacgtgg
     5881 aggatgtcgg cgtccacctt cagccgattg agcaaccctt tcaggtgtac gagctgtgcg
     5941 gcgattccga ccgtgtctac ggagccggcc gcgctcaacc aaatgcggct gcacccgcgc
     6001 acggcgagcc agctcgtcgc gttggtgtag gcgtgcgcgt ggcacacgac gttcttgccg
     6061 ttcttgtgaa agcgctcgaa gagctgaccc agctcctcgg cacgagaaaa atcgattccc
     6121 tgtgcgccga ggcgaacgta aaccgaggct gtcagcggat ccgtcgcggc gcgctcgagc
     6181 gctcgcacga gccccgtgta cgtccgcgtc gcgggcatct gaatcagccc gcctcgggac
     6241 gcctctggca tcccgctgcc gaggtcgacc gcgacgacgc gcgcctcttt ctctccgggt
     6301 tcgcgtgctt cgtccttgtt ttcgttcact cgcgggcgcc ccttgcagcc cacggcgagg
     6361 gcgagcgccg cgagcgcaat gactcgcttc atttgaaccc cggtagatcc gacgtatcga
     6421 cctcaggtgt gtcggaaggc ttttcttgag aggccggcgg tggcggcgcc ttctccgctg
     6481 gtttctcggt ggtgctcccg ccttgctccg cgatgcgcgc tgaggcgcgt tgcccgtagc
     6541 tgctgccgct cccgctctgc tcgacgacgc gcttgtaaaa ggagatggca gcggcgcgat
     6601 tgcccgaatc ccactcttgg tctccgcgcg ccatcagcgc cggcaggtag ttcggattgt
     6661 tcgcgagtac gcgatcgtag agtcgcgccg ccgtcgcggg atcgtggcgg cgccgcgcga
     6721 tgtcggcgag tccggcgagc gcctcggtgg cgttcggttg ggcagtgagc acacgctcgt
     6781 agagcgactc ggcctcggag aggctcccct tcgagagggc tttggatgcg tcggcgagct
     6841 gctttctgaa gtcgcccgag gaggcgcgag cagcttcgac gttcggcgtc gtggcgccct
     6901 tcgcggccgc cttgggctcc tcggcttgcg aggtgtcgag cttgccgagc ttgcccggat
     6961 ctaccatcgc cacgggcgcg ctcgggctcg cgtccttccc ggccgagtaa cgcttgagga
     7021 agccccgcag ctcgtcctgt agcagcggcg tcgaagcgct cgactcgagc ttgccgaact
     7081 cggtctcggc ctcggtgagc ttgccgccgc gcgcgagcgc gtagatgagc gccgcgtgag
     7141 cgcggccggc gttgcgctcc cccgacacgg ccacgcgcaa gcggtcgacg atactgcccc
     7201 agcccgggtt cgggtcggac aagtcgagcg cggcgagcac gtaggcgttt tggggatcgc
     7261 tcgggttcgc tgcgatgggc tttatccact cgcgagcttt gtctgcttca ccgctcaagc
     7321 gcagcgtgtc cacccgtgcg cgcagcacca cgatgtcttc cggcgcgacg gcgaaagcgg
     7381 cgtcggctgc gaggcgagcg cgcccgacgc gtcgaccgag ctcgcggtga gtcgcttgca
     7441 cgaggtcgcc gagctccgga tcgagcagac ggagcttgag ccagctgatg tcggcgcgga
     7501 tggtctcgag gtgagccagc gccgcgagca cggcgtgatc tttttcggcg agcgcgctgg
     7561 cgcgtaagag ctgctcgctc gcgccgtcca cgtcgccttc accgagcagg cgattgccat
     7621 cgcgcagcag cgtcgcgacc cgtgcgttgg aagcggccgc ttcttgcgag ctcgagccgg
     7681 cgttctcgag caggtaacgg cgacctaccg tcaacgcgaa cagcgtggtg acccccgcga
     7741 tgacgaacgc cgcgatccag cgggagcgcg ctcggcggcc gtgctcgtgg atctcctcgt
     7801 gggtgagctc gtcgtacgag cgcagatcgc gccgacccgc cgagatcggc cccgacgagt
     7861 agggcgagct cggcaccggc atgagctccg gcggaatcgg agcggccgcg aagggagagc
     7921 tcggcgtgag catgtactcg cggccggaga gcggcgcgtt ctgcgaagcc accggctgca
     7981 tttcagatac cgggagcgac gtcgagctcg gagctgcgga gttcgcccgc accgagacgg
     8041 gctcgaccgg aggcagcgct ggtgtcggtg ctgcgctgag cgatgactcg gggagctcct
     8101 cgcgctcggc ctcgaaagag gccgaagacg cttctccgag gcccggctgc gtgtggcgtc
     8161 gcgagtcctc gggtgcttcg tgcgctacgg gcccccgcgg ttcagcgctc gcgcgcggcg
     8221 gaatcgccga ggcgttggcg agcaccgggt gcggtggcgc gacccccgcg agcgtacggg
     8281 cgggccgagc gctcttttgg gcgctctcgt ggaagaacgg ttcgagctcg gcgatcgccc
     8341 cgagagcgcg cggtggctgt cgccctcgcg acaagagatc gttcggtccg actttctgat
     8401 ccgcgatgcc gcgctggagc tcgcgcagcg acgtatagac gagctcatcg ccgttcgcgg
     8461 ttcgcacgac ccagcgctcg ggctcttggc tctgactgcc ggtgggaaag accttgaact
     8521 ggtacccgca gttcgtacac tttacggtcg ttccgcgctc cgaaatgagg gcatcgtcga
     8581 actcgtactc ggtggcgcaa cggttgcagc ggacgtccat agaatctcgg ggtctcgtgc
     8641 agtcaggggt ctacgctgtc gagctctcgg ggtttcgcgg cgtggagccg gaagggaagc
     8701 cgttcgttcg gcgcaaacgt agcatgttcg ccgtagcatc gggactttct aggctccgcg
     8761 tgacgctcgc gccgcacggg gcggaacggc tccggcttgt cagggggagc ccagttgcta
     8821 ccctgccacg cgtggcgact ggctcttagg ctcgagagcc gtcagggacg atggtccgat
     8881 gaaacgcttt gctgtcgttg ttctcctttt agccgtcggt agcattctgc ccggttgccc
     8941 catctacgat gacgacagcg gctgctcagt cgatgaagat tgccctgtcg actacgcctg
     9001 cgacagtttg gtcggttcct gccgtcctaa cgcctgctcc gcgccgaccc aatgcccggc
     9061 tgaccagact tgctcgcgcg gaggcaagtg cgtcgcgctc gactgcagct ggcccgaggt
     9121 cgggtgcgtt gccggctaca cctgctcgtc gaggacgaag acctgggagt gcgtcaaggc
     9181 atcgaccggg ggcgaagggg gtaacggcgg tgccggcgca acggcaactg gcggcacggg
     9241 agagtcgagc ggcggcctga gtgctagcgg cggagcctcc cccgacgcga ctggtggaac
     9301 cccggcagac acctccggcg ggacgagcgg ctagctcggc ggcgttcgac tctcggggtg
     9361 gcgcagcttc gcgcgctgcg cgctacttga tgacgcgcag atacggcggc agttcgcgcc
     9421 gcgacttcgg cggctgactc gcgctcgact cctggcttgc gctagcgagc gccggctctg
     9481 gccgggggcg cgcgaccgct tcacgagccg gtgcgcgcgg agctttcgcc gctttcggct
     9541 ccttctcggc cgagtcggtg cgcttggctt ttcgcgcgcg cttcttcttc cccttggtga
     9601 cctcgccctc gccttcggcc gtggtcgccg gagcctcggg ttcgggcgc
//
LOCUS       DASTKS010001083         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1287330, whole genome shotgun sequence.
ACCESSION   DASTKS010001083 DASTKS010000000
VERSION     DASTKS010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287330"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VFQ35_22825"
     CDS             <1..1939
                     /locus_tag="VFQ35_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933565.1"
                     /translation="WAEKTSSEPRPEFAAFTPDLRPFQSLLQSLARTDAYRVSGYWKA
                     NVTLKEHDDGSYSYWSREPSAAAVRRQLKPSRGIAMLIGELQGVTPRKFDELVAHVLE
                     QLELDESTAIAALIHLAKAGVLVGGLQLPSRFDSAWDALAKAAEHLRPEHRRAWWRAY
                     LALRRICRQLENVSALTPSTLEVAFTRAQAAIEALATALGLECPTFPRVPLRADLRLG
                     FAFTLGRDFRERIERDLAEYSRFQSAYGLGQCLRRAAWHEELTRSATPVELNCIPPAE
                     EVTSPPEGPLTWDAYASLFSAGSQFRQRIRKWEALLTSGAATVTCEGEEEQPSPPFAC
                     FLFGGAPGAISIHGIAEELAPVYARYGLLWTQRSKRPRDELFAWYHNRLAAVQAEARI
                     RIVELVTPCEQNPNALARPAFTPETLSVWCADPSELPLAGSQFFVDPLSHAVFLQAPT
                     FKKPLAVFSFASANVGWSDPVCQRLLTTSFRVARLAHLRADSVPFRAELELGKASPEV
                     RIGTGAIVRARRSIITGTALVRWLALDAPARFAEWLRLAEENDWGSFVLVRRDDGHAL
                     LVPVQSPLALEAVLEGAAAAHMITIERFSEEAWIRDAEGKRYIAEFAVPFVRARNAWN
                     AEVAAHAELATSEHSFAAPAR"
     gene            1890..2732
                     /locus_tag="VFQ35_22830"
     CDS             1890..2732
                     /locus_tag="VFQ35_22830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET9933566.1"
                     /translation="MPNWLQANIRLRRRHGDPVASAAALFGELRVAVKAWRASGAVQR
                     CYFVRKPPDVRLRLEFSSVEAEIEIVRWLDARRGRSITSWTRACYEPEAALLGGAHLL
                     DHVHAYFDVDTTAFMDFWRPFGDASLGPCADERAGLSALALSLSVLNDLFVRAVDGRE
                     EVWDVWWNLASLHGGATPSHEPSATPVKIDDLLGRVPAYDERILKRYRRANTSLANAM
                     RRLGARGKLLFGRRSVLPFVALFHWNRYGFALEERARVFSAMMSAWDPGRQFHGRFEA
                     SGAT"
     gene            2729..>3085
                     /locus_tag="VFQ35_22835"
     CDS             2729..>3085
                     /locus_tag="VFQ35_22835"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9933567.1"
                     /translation="MTLRRTSWFAAAFFGALGVARAQSAPPRVVTFREALELAQSHPN
                     VRTARARVEAAGASVDVARAARNPQLTLGLQAGVTAIDQPIVPESDIRIQATGALAQA
                     TLNGQWMLYDFGRVSSM"
BASE COUNT          501 a         1036 c          989 g          559 t
ORIGIN      
        1 ctgggcagag aaaacctcga gtgagccgcg ccccgaattc gccgcgttca cgccggatct
       61 gcggccattt cagagcctcc tccaatcgct ggctcgcacc gacgcctatc gagtgagcgg
      121 ctactggaag gcgaacgtca ccttgaagga gcacgacgac ggctcctact cctactggtc
      181 ccgcgaacct tcagccgcgg cagtccggcg gcagctcaag ccgagccgcg gaatcgcgat
      241 gctcatcggc gagcttcaag gagtcacgcc gaggaagttc gacgaactcg tcgctcacgt
      301 cctcgagcag ctcgagctcg atgaatccac cgcgatcgcc gcgctcatcc acctcgcgaa
      361 agcgggagtc ctggtcggag ggctgcagct accgagccgg ttcgattcgg cgtgggacgc
      421 cctggccaaa gcggcagaac acctccgccc agagcaccga cgcgcgtggt ggcgagcgta
      481 ccttgcgcta cgaagaatct gccgccagct ggaaaatgtg agcgccctca cgccgagcac
      541 gctggaagtg gccttcactc gagcgcaggc ggccatcgaa gctctggcta ccgcgctggg
      601 cctagagtgc cccacgttcc cccgagttcc tctgcgcgcc gatctgcgtc tcggcttcgc
      661 tttcaccctc ggccgcgatt ttcgcgaacg aatcgagcgc gacctcgcgg agtattcgcg
      721 gtttcagtcc gcttacggct tgggtcaatg cttgcgaagg gccgcttggc acgaggagct
      781 cacgcgttcc gcgacgccgg tggagctcaa ctgcatcccc cctgcagaag aggtgacctc
      841 gcccccggaa gggcctctca cttgggatgc ttacgcgtcg ctcttcagcg ctggttcgca
      901 atttcgtcaa cgcattcgca agtgggaagc gctgctcacc tcgggagccg ctaccgtcac
      961 gtgcgaggga gaagaagagc aaccgtcgcc gcccttcgcg tgctttctct tcggcggcgc
     1021 gcccggcgcc atttccatcc atggcatcgc ggaggaactc gcgccggtgt acgcgcgcta
     1081 cgggctcctc tggacccagc gctcgaagcg gccccgtgac gagctattcg cctggtatca
     1141 caaccgtctc gccgcagttc aggcggaagc gcgcatccgc atcgtcgagc tcgtgacccc
     1201 ctgtgagcaa aaccccaatg ccctcgcacg cccggcgttc acgccagaaa cgctcagcgt
     1261 ttggtgcgcg gatccgagcg agctgcccct cgctggctcc cagttcttcg tggacccact
     1321 gtcccacgcc gtgttcttgc aggctccgac tttcaagaag cctctcgcgg tgttctcgtt
     1381 cgcgtcggcc aatgtgggct ggagcgaccc ggtttgccaa cggctactca cgacgtcgtt
     1441 ccgtgtggcg cggctggcgc atctacgcgc cgacagcgtc ccgttcaggg ctgaactcga
     1501 gctcggcaaa gcttcacccg aggtgaggat cggcaccggc gccatcgtcc gcgcgaggcg
     1561 cagcatcatc accgggaccg cgctggttcg ttggctcgcg ctcgacgcgc ctgcccgttt
     1621 cgccgagtgg ttacggctgg ccgaggaaaa cgactggggc agcttcgtgc tcgtgcgccg
     1681 cgacgacggt cacgctctac tggtccccgt gcaaagcccc ttggcactcg aagcagtgct
     1741 cgagggggca gcggcggcgc acatgattac catcgagcgg ttctccgagg aggcttggat
     1801 tcgggatgcg gaaggcaaac gctacatcgc agaatttgcc gtacccttcg tgcgagctcg
     1861 aaacgcttgg aacgccgaag tcgcggccca tgccgaactg gctacaagcg aacattcgtt
     1921 tgcggcgccg gcacggtgat cccgtcgcga gcgccgccgc tctgtttggc gagcttcgcg
     1981 tggcggtaaa ggcgtggcgc gcgagcgggg ccgtacagcg ctgctacttc gtgcgaaagc
     2041 cccccgacgt ccgactccgt ctcgagttct cctccgtgga ggccgaaatc gagatcgtgc
     2101 ggtggctcga cgcgcgacgt gggcgttcca tcacgagctg gacccgtgcc tgctacgagc
     2161 cggaggccgc actcctcggc ggcgcccatt tgctcgacca cgtgcacgcc tacttcgacg
     2221 tcgacacgac ggcgttcatg gacttctgga ggccgttcgg cgacgcttcg ctcgggccgt
     2281 gcgccgacga gcgtgcgggg ctctcggcgc tcgccctctc cctcagcgtg ctgaacgact
     2341 tgttcgtacg ggccgtagac ggccgcgaag aggtttggga cgtctggtgg aacctggcgt
     2401 cgctgcatgg tggggcgacg ccgagtcacg aaccttcggc aactcccgtg aagatcgacg
     2461 atctcctcgg aagagtgccg gcgtacgacg aacgtatttt gaagaggtac cggcgagcca
     2521 atacgtcttt ggcaaacgcc atgcggcggc tcggtgcgcg cggcaaactc ctcttcggtc
     2581 ggcgctccgt cttgccgttc gtcgcgctgt ttcactggaa tcgctacggc tttgcgctcg
     2641 aagaacgcgc gcgcgtgttc tctgcgatga tgagcgcatg ggatccgggc cgccagtttc
     2701 acggtcgatt cgaggcaagc ggagcgacat gaccctgcgc cgaaccagct ggtttgcggc
     2761 agcgttcttc ggggcactcg gggttgcccg cgctcagtcc gcgccgccgc gcgtggtgac
     2821 tttccgcgaa gcgctcgagt tggcccaatc acaccccaac gttcgtaccg cgcgtgcgcg
     2881 agtcgaggct gccggcgcca gcgtggacgt ggcccgtgcg gcgcgaaacc ctcaactgac
     2941 gctcggcttg caggcgggag tcacggcgat cgatcaaccg atcgttcccg agtcggacat
     3001 tcgaatccaa gcgacgggtg cgctcgcgca ggcgacgctg aacggccaat ggatgctcta
     3061 cgacttcggg cgtgtgtcga gcatg
//
LOCUS       DASTKS010001084         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_392283, whole genome shotgun sequence.
ACCESSION   DASTKS010001084 DASTKS010000000
VERSION     DASTKS010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392283"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFQ35_22840"
     CDS             <1..637
                     /locus_tag="VFQ35_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9933568.1"
                     /translation="FKEFGGGKDAMPKLTQLVMDFSHLFVSALPLLIVFGILATVGFA
                     YFYRTPRGKRLVHQLLLKLPVMGPVLRKIAVARFTRTLGTLLQSGVPILDALEICAKT
                     SGNVIIESGIMSVRQAISEGRNMAEPLTQTQIFPDMVVQMIAVGEQTGALDQMLNKIA
                     DFYEEETDVAVAAMTSALEPIMMVGVGGMVGIVLISMYLPIFSLAGNIKAD"
     gene            634..1941
                     /locus_tag="VFQ35_22845"
     CDS             634..1941
                     /locus_tag="VFQ35_22845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933569.1"
                     /translation="MKIQLGLPAFVNAGPLPRRLAWLTGARLMFLLLALALVGTFYLR
                     DAFDIGSYTLRVTVAMLAGSFAAAGAYAVILRSGRGLERLADVQLVVDQITWTFVAYL
                     TGGVTSGATSFYGLSCLVGASLSGMRGAAVAAVAAALSYGGMVLSLTRGWVAPPRDQA
                     LSLYLVSTQEASFYFIVNLLVLVVVALLAGTLVERLRWTGGELVLATERADRAERMAA
                     LGRLAAGLAHEIRNPLGSISGCIQLLKTVPNLSEEDRKLCDIIQREAARLNDLVTDMV
                     DLSRPRKPMLASVDVANLAREVVALSSGSGRGVSDVAVRYEGLDRAEVQADGAQLRQL
                     VWNLVRNAVQASRPADEVVVATRRDDHGAIELSVSDKGPGIDPTAHERLFDAFFTTRS
                     QGTGVGLAVVKRIADEHGFAIRVESSAGQGATFRVVLQPTAST"
BASE COUNT          353 a          710 c          747 g          420 t
ORIGIN      
        1 gttcaaggag ttcggtggcg gcaaggacgc catgccgaag ctcacgcagc tcgtcatgga
       61 cttttcgcac ttgttcgtgt cggctctgcc gctactgatt gtgttcggca tcctcgcgac
      121 ggtcggtttc gcgtacttct accgaacgcc acgcgggaag cgcctggtcc atcaactcct
      181 gctgaagctc cccgtgatgg gccccgtgct ccgcaagatt gcagtggcgc ggttcacgcg
      241 cacgctcggc acgctgctcc aatccggcgt ccccatcctc gacgctctcg aaatctgcgc
      301 gaagacttcc gggaacgtca tcatcgagtc gggcatcatg tcggtgcggc aggccatcag
      361 cgagggccgc aacatggccg agccgctcac tcagactcag atctttcccg acatggtggt
      421 gcagatgatc gcggtcggtg agcagaccgg cgccctcgat cagatgctca acaagatcgc
      481 cgatttctac gaagaagaga cggacgtcgc ggtcgcggcg atgaccagcg cgctcgagcc
      541 catcatgatg gtgggggtcg gcggcatggt cggcatcgtg ctgatttcga tgtacttacc
      601 catcttctcg ctcgccggta acatcaaggc cgattgaaga ttcagctcgg gcttccggcg
      661 ttcgtcaacg cggggccact gccgcggcgg ctcgcgtggc tcaccggggc ccggttgatg
      721 ttcctgttgc tcgcgctcgc gctcgtcggg acattctacc tgcgcgacgc attcgacatc
      781 ggttcgtaca cgctgcgcgt aaccgtcgcg atgctcgccg gctcgttcgc ggccgcggga
      841 gcctacgccg tcattttgcg cagcgggcgc ggcctcgagc ggctcgcgga cgtgcagctc
      901 gtcgtcgatc aaattacgtg gaccttcgtc gcttatctga cgggtggggt cacgagcggt
      961 gcgacgtcct tctacggctt gagctgcctc gtcggcgcga gcctgagcgg aatgcgcggc
     1021 gcagccgtcg cagccgtcgc ggccgcgctt tcctacggcg gcatggtgtt gagcctcacg
     1081 cggggctggg tcgcgccgcc aagggaccag gctctgagcc tttatctggt ctccacgcaa
     1141 gaggcatcgt tttacttcat cgtgaacctg ctcgtgctcg tggtggtcgc gctgctcgcg
     1201 ggcacgttgg tcgagcggct ccgctggacg ggtggggagc tcgttctcgc gaccgagcga
     1261 gccgatcggg ccgagcggat ggcagccctc ggccgactcg ccgcagggct cgcgcacgag
     1321 attcgaaacc ccctcgggtc gatctccggc tgcatccagc tcttgaagac ggttccgaac
     1381 ctatcggagg aagaccgcaa gctctgcgac atcatccagc gcgaagcggc tcggctgaac
     1441 gacttggtga ccgacatggt cgacctttcg cggccgcgca agcccatgct cgcgagcgtc
     1501 gacgtcgcga acctggcgcg cgaggtcgtc gccctttcgt cgggctcggg gcgcggtgtc
     1561 tcggacgtcg cggtgcgcta cgaagggctc gatcgggcgg aggtccaggc ggacggcgcg
     1621 cagctccggc agctggtttg gaacttggtg cgaaacgcag tgcaagcgtc gcggcccgcg
     1681 gacgaagtcg tggtcgcgac gcgacgcgac gaccacggag ccatcgagct gagtgtgagt
     1741 gacaaagggc ccggtatcga ccccaccgcg cacgagcgct tgttcgacgc tttcttcacg
     1801 acgcgctctc aagggaccgg cgtggggttg gcggtggtga aacgcatcgc tgacgagcat
     1861 ggatttgcca ttcgggtcga gagctccgct gggcagggcg cgaccttccg cgtcgtgctc
     1921 cagccgaccg cgtcgacgtg acgtgagcga cgagggagca cctcggttcc gtcctcttcg
     1981 gaacgtcggc tgccggcgag tcttccctcg tcatgagccg cgcaagcaac actggaatcg
     2041 cgctgtcgat cggtgtctga gccaagccac gctgtagctt cgagttcacg tcgttgcggc
     2101 ggagagcggc agggactgca gaactgccgc atttgcacgc ggctcagctc gaagtgggcg
     2161 aaattcgcag ctgcggcgcg cgcggtggac caaaaacgcc acgaaattcg tacggttcag
     2221 cgctcgcaaa
//
LOCUS       DASTKS010001085         7017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1511441, whole genome shotgun sequence.
ACCESSION   DASTKS010001085 DASTKS010000000
VERSION     DASTKS010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511441"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFQ35_22850"
     CDS             <1..1043
                     /locus_tag="VFQ35_22850"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9933570.1"
                     /translation="NPEAPELAHVRTRGASIAVDAAISNSFGFGGMDSVVLFEAASAP
                     ARRRAELSPRLFVSAAVPFVELESVDARDSLDPERSRRFDRVTATSARLAELALEKAG
                     LRARDVGVVLGGAYGPVERSLTFLDRILSRGLKFAAPAEFPHLVNSAATGNVSIYLRA
                     RGPAVAVSALGVSGEAAFDVACSLAELGTTEAIVACALELSDPVVTELFTKEHASSSP
                     RGQGGAALLVETDGSWRRRGGAPLARVERVRAFRGAPSLLEFEFSGDAASSAVVFSEH
                     TDELFRAVEGSSWANAARFRQDAHGYHEALGASALAHAVSLIGAGKRRVLVLGADSAL
                     TFAIELARPEEP"
     gene            1040..2836
                     /locus_tag="VFQ35_22855"
     CDS             1040..2836
                     /locus_tag="VFQ35_22855"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9933571.1"
                     /translation="MRPVSVLASAAISALGRGTRAAHVGEIGEEPPTAVRLEPAGALA
                     RIDDAWLDVGEGDRAERLLSSAARDLGRRLDELAPDFRARRLFVCVGTSAGAMHSMQA
                     AFASLEGAPQSLARRANYFAPLDGLFSSLGLSRASVKCVQPLGACSSSTLAIGLACRA
                     LEAGDCDLAIAGGYDALSDFVISGFAALGALSLRGPAPFRVERDGLALGEGAALIALV
                     RAPEEARAPCILGFGASADAVHITAPDRDGRGLARAAERALLDAGIVAREVDLVSAHG
                     TATPYNDSAESKALAQLRSEQRAVVHPWKASIGHTLGAAGALEVLAAFDALTRGVLPG
                     ARGAGEKEPELAGELLETNSAGAPRVALKLSAAFGGLNAALVLGAAGLQSPGSALAFR
                     PVELAALGESVTTGEPELVASLAPNARDLAARADSLSELVLAAVARLVRSLGAPLPES
                     CAVIVGTGVATLEANERFDQRRRADLPVLPRAFPPTSPNLCAGICSIAWGLRGPAFSV
                     GASLMGAGREAFDVAELLVAAGDAEAALVVLAEDAGPVVERLFRCAGEPVPSRRARAA
                     LLVPGVVGNGGSEPNRHRDSAARWAELAAAAP"
     gene            2962..3444
                     /locus_tag="VFQ35_22860"
     CDS             2962..3444
                     /locus_tag="VFQ35_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933572.1"
                     /translation="MNRVWYRLFALALGLLPLTGCATHYIPNTDVEDNDFNRELIEFC
                     EKYRHAVEYRNVPLLLQMAHDNYYEDGGTVDTSDDIDKAGLAEYLQGRFKDTTGIRYE
                     IRYRAVSPGRKQTFFVDFTYSASYKIPTSEGEAWRRRVADDRLELVRDGESFKILSGM
                     "
     gene            complement(3458..4084)
                     /locus_tag="VFQ35_22865"
     CDS             complement(3458..4084)
                     /locus_tag="VFQ35_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933573.1"
                     /translation="MNARRLAAAALLASACGGGSARFPTTDPNEEPPRRRPPGIAVDP
                     VSRLPEVKGRSDSRASLVVLGAPRSATLARGIVARYFRALVGESPEALDPLLSEQAFI
                     DTSGSRQPARGALRSRFLQLDYTALRGVPLYRDADLEIYRAEDAEALRAARSLPTDIG
                     KDQLFVRVRLNVSHAGKTRVFANEMGFLLRPEQEDFRIVSIREDTPVP"
     gene            complement(4081..4902)
                     /locus_tag="VFQ35_22870"
     CDS             complement(4081..4902)
                     /locus_tag="VFQ35_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET9933574.1"
                     /translation="MFGFIGNRSDLGALVLKSHADLLRVRRAPGEPLGWGLGFYQAGE
                     ILLRRRPIDDRNEIDLTEAAEDIRTDVLIGHVRRASVGALRTENTHPFRYRSWVFAQT
                     GTIGGFENLRERLLASQPEFLRRNVRGETDSECFFYLFLSFLHDAGHLDEGHVAMNHV
                     AAALRASISLVDRLSAEEGYGPNGGNIMVTNGESMLAVHRDGMLGYRLLRGKFDVEGL
                     LGEDGMRRARIPSIDSTHFTILASDLDALPAGWTSVSERAIVTLSRTEDPRVEPL"
     gene            5057..5773
                     /locus_tag="VFQ35_22875"
     CDS             5057..5773
                     /locus_tag="VFQ35_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9933575.1"
                     /translation="MDLADFKEALLAIMDQKTHWAWPAFTSGRVARERLNVHLEQEYA
                     TYVRDFPVLVGRAYVACPIAEARRELIANVYEEETGGLFAGRPHPELFLEYPRGLGMD
                     LTRFEHVVLLPKAAEYRRVLDEATLHSGWEAAAAVTTLFVEGTASERAELNDGGRRSL
                     PPLEEHPLVRHYGLPLEHLALTKAHRSVEGDHRRSAWHVILSFVSEGARTGVVEAMKS
                     ALFAWRAYRDDVAAACGVQR"
     gene            5900..6742
                     /locus_tag="VFQ35_22880"
     CDS             5900..6742
                     /locus_tag="VFQ35_22880"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9933576.1"
                     /translation="MKSNRSFPLAVRGALLRNIALSVVMGLGVVASACGAVFPELTPP
                     IRQPPPGFVFEPSPPPDLLYIEFEKATIPSKTRDGRAWDSVGGSLPDPIAKLFVNDDE
                     VIVTPLQANTLNPTWPNQKRGNYRIPEDARLRVELWDSNPLNNRPICRQDVRELHSHL
                     SDSGSLEVTCDSGAYVKILAQPAHGKLGLGFSYEIRTDEVFIARVWVESPAARAGIKR
                     GDQVISIMGEAVKGMPEGRVRSLINSNGSSGLSLRVRGVDKQERDVVVKDGPIYPLLS
                     EETL"
     gene            6739..>7017
                     /locus_tag="VFQ35_22885"
     CDS             6739..>7017
                     /locus_tag="VFQ35_22885"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933577.1"
                     /translation="MTILDALTGAVVLPDSHRTLRVTGKDAVSWLNGIVTCDCASVDE
                     RRGTLGLLLTKQGKIVTDLEIVRSGESLLLGVDANLFDSVRELLDRYLV"
BASE COUNT         1097 a         2276 c         2411 g         1233 t
ORIGIN      
        1 aaaaccccga ggcgcccgag ctcgcgcacg tgcgtactcg cggcgcctcc atcgctgtcg
       61 acgccgccat ttcgaactcg ttcggcttcg gcggcatgga ttcggtggtt ttgttcgagg
      121 cggcttccgc gcccgcgcgc cgacgcgccg agctctcgcc tcggctcttc gtgagcgccg
      181 cggtcccgtt cgtcgagctc gaatcggtgg acgcgcgcga ctcgctcgac cccgagcggt
      241 cgcgtcgctt cgatcgggtg accgcgacgt cggctcgtct ggccgaactc gcgctcgaaa
      301 aggctgggct ccgcgcgcgt gacgtgggcg tcgtgctcgg cggcgcctac ggccccgtcg
      361 agcggtcgct gaccttcttg gatcggatct tgagccgcgg gctcaagttt gctgcgccgg
      421 cagagttccc acacttggta aactccgcgg ctacgggcaa cgtatcgatt tacctcagag
      481 cgcgcggccc tgcggtagct gtctccgcgc tcggggtgag cggcgaggcg gcgttcgacg
      541 tcgcctgttc gctcgccgag ctcggcacta ccgaggcgat cgtcgcctgt gcgctcgaat
      601 tgagcgatcc cgtcgtgacg gagctgttca cgaaggaaca cgcgagtagc tccccgcggg
      661 gccagggtgg cgccgcgttg ctcgtcgaga cagatgggtc atggcgcagg cgagggggcg
      721 cgccgctcgc gcgggtcgag cgcgttcgcg cgttccgggg agcgccgagc cttttggagt
      781 tcgagttctc gggcgacgcg gcgtcgagcg cggtggtgtt ctcggagcac acggatgagc
      841 tatttcgagc cgtcgagggc tcgtcgtggg cgaacgcggc gcgttttcgg caagacgcgc
      901 acggttatca cgaagcgctc ggagcgagcg ctttggcgca cgccgtttcc ctaatcggag
      961 cgggcaaacg ccgtgtgctc gtgctcggag cggactcagc cctgacgttc gccatcgagc
     1021 tcgctcgccc cgaggagccg tgaggcccgt ttcggtgctc gcgagcgctg cgatttctgc
     1081 gctcggccgc ggcacgcgag ccgcccacgt cggtgagatt ggcgaagagc cgcccacggc
     1141 ggtgcgactc gaacccgccg gcgcgctggc tcgaatcgac gacgcctggc tcgatgtggg
     1201 cgagggcgat cgcgcggagc ggctgctttc gagcgctgcg cgcgacctcg ggaggcggct
     1261 cgacgagctc gcgccggact ttcgcgctcg acgtcttttc gtctgcgtcg gaacctccgc
     1321 gggcgcgatg cactcgatgc aagcggcgtt cgcgtcgctc gaaggcgcgc cgcaaagcct
     1381 cgctcggcga gccaactact tcgcgccgct cgacggccta ttcagctcgc tcgggctctc
     1441 acgcgcttcg gtgaagtgcg tgcaacctct gggggcgtgc tcttcctcca ctctggccat
     1501 cgggctcgct tgtcgcgcgc tcgaggcggg cgattgcgac ctcgcgatcg cgggtggcta
     1561 cgacgcgctc agcgatttcg tgatctcggg tttcgccgcg ctcggcgcgc tcagtctgcg
     1621 ggggcccgcg ccgtttcgcg tcgagcgaga tggtctcgcg ctcggcgaag gggcggcgct
     1681 gatcgcgctg gttcgcgcgc cggaagaagc ccgggctccc tgcatcctcg ggttcggcgc
     1741 gagcgcggac gcggttcaca tcaccgcgcc agaccgcgac ggtcgaggcc tcgcgcgcgc
     1801 cgcggagcga gcgctgctcg acgcggggat cgtggcgcgc gaagtcgacc tcgtgagcgc
     1861 gcatggcacg gccacgccct acaacgactc ggccgaatcc aaagccctcg cgcagctccg
     1921 ttccgagcaa cgcgcggtcg tgcacccgtg gaaggcgagt atcggacaca ccttgggggc
     1981 agcgggcgcg ctcgaagtgc ttgccgcatt cgacgcgctc acccgcggcg ttttgcctgg
     2041 cgcgcgtggc gctggggaaa aggagcctga acttgccggc gagctcctcg agacgaactc
     2101 cgcgggagca ccgcgggtcg cgctgaagct ctccgccgca ttcggcgggc tgaacgctgc
     2161 gctcgtgctc ggagcggcgg gactgcagag cccgggctcg gcgctcgcgt ttcgtccggt
     2221 ggagctcgcc gccctcggag agtcggtcac gactggcgag ccagagctcg tcgcatcgct
     2281 cgcgccgaac gcgcgcgatc tcgcagcgcg cgccgattcg ctttcggagc tcgtactcgc
     2341 ggcggtggcg cgcctcgtac ggtcgctggg cgcgccgctg ccagagtctt gtgcggtgat
     2401 cgtcgggacg ggggtcgcga cgctggaggc caacgagcgc ttcgaccagc gccgccgcgc
     2461 ggatttgccc gtgctgcccc gcgcctttcc ccccacgtcg ccgaacctgt gcgctggcat
     2521 ttgctccatc gcgtgggggc ttcgcggtcc agctttcagc gtcggggcat cgttgatggg
     2581 cgcggggcgc gaggctttcg acgtagcgga gctcttggtc gcggctggcg acgcggaggc
     2641 ggcgctcgtc gtgctggccg aggacgccgg gccggtcgtg gaacggcttt ttcggtgcgc
     2701 tggcgaaccc gtgcccagcc gtcgcgctcg ggcggccctg ctggtcccgg gtgtcgttgg
     2761 gaacgggggc tcagagccga accgccaccg tgattcggcc gccaggtggg cggagctcgc
     2821 agcggccgca ccctgagcgc gcctggcttc aggcgcgtat ttgtgccacg acccgcttgc
     2881 ccaaagtcgc cggtctcttt taccgtcccc gggaattggc cggcgccgaa cggcgttcga
     2941 acggcgtcgg aaagcatcct catgaatcgg gtctggtaca ggttgttcgc gctggctctt
     3001 ggtctcctgc cgctcacggg ctgcgctacc cactacatcc cgaacaccga cgtcgaggac
     3061 aacgacttca atcgggagct catcgagttc tgcgaaaagt accggcacgc cgtcgagtac
     3121 cgcaatgtgc cgttgttgct gcagatggct cacgacaact actacgaaga cggcggcacc
     3181 gtggacacga gcgacgacat cgacaaggcg gggctcgccg agtacctcca gggtcgcttc
     3241 aaggacacca ctggcattcg ttacgagatc cgctatcggg ccgtctcgcc aggccggaag
     3301 caaaccttct tcgtcgattt cacgtacagc gccagttaca agatcccgac ctccgagggc
     3361 gaggcctggc gacgccgcgt cgccgacgat cggctcgagc tcgtgcgcga cggcgagagc
     3421 ttcaagatcc tgtccggaat gtgatcgaac gctcggccta cggcaccggc gtgtcttcgc
     3481 ggatgctcac gatccgaaag tcttcttgtt cgggtcgaag cagaaagccc atttcgttcg
     3541 cgaacacgcg cgtcttgccc gcgtgcgaaa cgttgaggcg cacccgcacg aagagctgat
     3601 ctttgccgat gtcggtcggc agtgagcgcg ccgcgcgcag ggcttccgcg tcctccgcgc
     3661 gatagatctc gagatcggcg tcgcgataga gcggaacgcc gcggagggcc gtgtagtcga
     3721 gttgcaagaa gcgcgaacgc agcgcgccgc gcgcaggctg tcggctgccg gaggtgtcga
     3781 tgaatgcttg ctcgctgagc aaggggtcga gcgcttcggg tgactcaccg accaacgctc
     3841 gaaagtaacg ggcgacgatg ccgcgcgcga gcgtcgcgct gcgtggcgcg cctagcacga
     3901 ccaacgaggc ccgggagtcg ctccggccct tcacctcggg cagccgtgac acgggatcga
     3961 ccgcgatgcc gggcggacga cgacgcggcg gttcctcgtt cggatcggtg gtcggaaaac
     4021 gcgcgctgcc accgccgcaa gcgctcgcga gtagcgccgc ggctgccaag cgacgcgcgt
     4081 tcacagcggc tcgacgcgcg gatcttcggt gcgcgaaagc gtgacgatcg cgcgctcgct
     4141 cacggaggtc cagccagccg gcagcgcgtc gagatccgaa gcgaggatgg tgaagtgcgt
     4201 gctgtcgatg ctcgggatgc gagcgcgccg catgccgtct tcgccgagga gcccctcgac
     4261 gtcgaatttg ccgcgcaaca ggcggtaccc gagcatgccg tcacggtgca cggccaacat
     4321 cgactcgccg ttggtgacca tgatgttccc gccgttcggg ccgtagccct cctcggccga
     4381 taggcgatcg acgagcgaga tcgacgcgcg cagcgcggcc gcgacgtgat tcatcgcgac
     4441 gtgcccttcg tcgaggtgtc cggcgtcgtg caagaacgaa aggaacaaat agaagaagca
     4501 ttcgctatcg gtctcgccgc ggacgttgcg gcggaggaac tccggttgcg aggcgagcag
     4561 tcgctcgcgc aagttctcga agcccccgat ggtgcccgtt tgcgcgaaca cccaagaccg
     4621 gtagcgaaac gggtgcgtat tttccgtgcg gagcgcgccg acgctggctc gtcgcacatg
     4681 tccgatcaag acgtcggtgc gaatgtcttc tgccgcttcg gtcagatcga tttcgttacg
     4741 atcgtcgatc ggtcgtcgcc tcaaaaggat ttcaccggct tgatagaaac cgagtcccca
     4801 cccgagcggc tcacctggcg cgcgacgaac ccgcaacagg tccgcgtgtg acttcaagac
     4861 gagcgctccg aggtcggaac ggttgccgat gaacccgaac atccgtgcca tcccttggaa
     4921 tgataccgct caccggcgct gtcggcatcc tcgctgacgc gagttgtatg ttacggcgtg
     4981 agggccggcc tgacagccga ggcgcgccgt ttcgacgctc gcgctcgcgt gtcaggtaag
     5041 ctagctgttc ttcagcgtgg acctcgccga cttcaaagaa gcgttgctcg cgatcatgga
     5101 ccaaaagacg cattgggcgt ggcccgcgtt cacgtcaggc cgggtcgcac gcgagcggct
     5161 gaacgtccac ctggagcaag aatatgcgac ctacgtgcgc gatttcccgg tattggtcgg
     5221 tcgcgcgtac gtcgcgtgcc ccattgccga agcgcggcgc gagctcatag cgaacgtgta
     5281 cgaagaggaa acgggtggac tattcgctgg gcgcccacac ccggagctgt ttctcgagta
     5341 cccgcgaggg ctggggatgg accttacgcg cttcgagcac gtcgtgctcc taccgaaggc
     5401 tgccgagtat cgacgggtct tggacgaggc cacgttgcac tctggctggg aagccgcggc
     5461 cgcagtcacg acgttgttcg tggaaggcac cgcgagcgag cgcgccgaac tgaacgacgg
     5521 tggccgacgc tcgcttccgc cgctcgagga gcatccgctc gtgcggcatt acgggttgcc
     5581 tctcgagcac ttggctttga cgaaggcgca tcgctcggtc gaaggcgacc accgccgttc
     5641 agcttggcat gtgatcctca gcttcgtcag cgaaggcgcg cgaacgggtg ttgtcgaagc
     5701 gatgaagagc gccctcttcg cctggcgagc gtaccgcgac gacgtggcgg cggcctgcgg
     5761 cgttcagcgc taggtttgca gtgtggtcga aattcgacga cggaacgacc ctctagcatt
     5821 cgaggctcag gggcgcgaaa gtcgggcgga ttcaggcagc cgaaccgggt tcgcgctgct
     5881 cgtgggaatg gagctgagct tgaagagtaa tcgtagtttc ccgctcgccg tgcgtggcgc
     5941 gctgcttcgt aatatcgcgc tctcggtggt gatggggctc ggggtagtcg cgagtgcgtg
     6001 cggagctgtg tttccggagt tgacgccgcc aatccgccaa ccgccgccgg gtttcgtctt
     6061 cgagccatcg ccgccgcccg acctcctgta catcgagttc gagaaggcga cgattccgtc
     6121 gaagacgcgt gacggacgag cctgggactc ggtgggcgga tctcttccgg acccgatcgc
     6181 caagctgttc gtgaacgacg acgaggtcat cgtgacgccc ctgcaagcga acacgctgaa
     6241 cccgacctgg ccgaaccaaa agcgtggcaa ctaccgcatc ccggaagacg ctcgactgcg
     6301 cgtcgaactc tgggacagta accccttgaa caatcgcccc atctgccgcc aagacgtgcg
     6361 cgagctgcac agccacctgt ccgattccgg cagcctcgaa gtgacctgtg acagcggggc
     6421 ctatgtgaag attctcgctc agcccgcgca cggcaagctg gggctcggct tctcttacga
     6481 gattcgcacc gacgaggtgt tcatcgcgcg cgtgtgggtc gaatctccag ctgctcgagc
     6541 cggcatcaag cgcggcgatc aggtgatcag catcatgggc gaggcagtca agggcatgcc
     6601 cgaagggcgg gtgcgtagcc tcatcaactc gaacggctcg agcggactgt ccctgcgtgt
     6661 gcgcggtgtc gacaagcagg agcgcgacgt ggtggtaaaa gacggtccca tttacccgct
     6721 acttagcgaa gaaacgttgt gaccatcctc gatgctttga ccggtgcggt ggtgttgccg
     6781 gactcgcacc gtacgctgcg agtcactggc aaagacgccg tttcgtggct aaacggcatc
     6841 gtcacgtgcg actgcgcgag cgtcgacgag cggcgaggca cgttggggct gctcctgacc
     6901 aagcaaggca agatcgtcac cgatctcgag atcgtgcgct ctggagaaag cctcttgctc
     6961 ggcgtcgacg cgaatctgtt cgactccgta cgcgagctcc tggaccgcta tctcgtc
//
LOCUS       DASTKS010001086         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1958279, whole genome shotgun sequence.
ACCESSION   DASTKS010001086 DASTKS010000000
VERSION     DASTKS010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958279"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFQ35_22890"
     CDS             complement(<1..238)
                     /locus_tag="VFQ35_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933578.1"
                     /translation="MRKITLAGLDAHVVGGSDGKGGGNGPVVVLLHGFGAPGTDLVPI
                     GDELPVDSDVRFVFPMAPHVLDPYSPPNAAARAWW"
     gene            346..756
                     /locus_tag="VFQ35_22895"
     CDS             346..756
                     /locus_tag="VFQ35_22895"
                     /inference="COORDINATES: protein motif:HMM:NF021119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HET9933579.1"
                     /translation="MDLKGLVTELLSELGKVSQSDAVVGAVRDAGKAKVLPLSRISIG
                     FGTATGEVGGTSQATGASEATAAGGGAGGAIVVEPRAFVVVGEDGVPHMLALERGKTA
                     VARPGVRILPSGESARELTGGAAPQLEAPKSSDG"
     gene            764..1429
                     /locus_tag="VFQ35_22900"
     CDS             764..1429
                     /locus_tag="VFQ35_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HET9933580.1"
                     /translation="MQLWLVLLASALCLGALAALAAIELEFEAQGVGEASGAWAAAFG
                     LTFAGVLVSGVLAREQRLKLEVRILGRKLDLQRLRKRRAPKPPKEKPQQPSKPRRRLP
                     IGLSEGIDLALLELRRIEVGRVDAAVTYGFRDIALTGRLAGALYALSGILPPTVHFNQ
                     TVKWDGAERWEASASGRVALWPGRVLFDVVWFMLRARWRRRSPTESREPSPEPSVLGG
                     SKP"
     gene            1426..1899
                     /locus_tag="VFQ35_22905"
     CDS             1426..1899
                     /locus_tag="VFQ35_22905"
                     /inference="COORDINATES: protein motif:HMM:NF021119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HET9933581.1"
                     /translation="MSKSNISEIVSSLLSGVHGISRSETIIGAPQQAGEATVIPVHRL
                     KIAFGAASATAGARGGKVGGDSGGHGAGGAVELEPVAAIAVGKDGAAHLLTVEGEQQN
                     AWSQLLQDAPDLLSKVLHSVGERMRHELAARGAPPVPEVAQAEAAKALTEGEKGR"
BASE COUNT          332 a          628 c          739 g          302 t
ORIGIN      
        1 gccaccaggc gcgggcggca gcgttcggcg gcgagtaggg gtcgagcacg tggggcgcca
       61 tcgggaacac gaagcgcacg tccgaatcga cggggagctc atcgccgatg ggcacgagat
      121 ccgtccctgg cgcgccgaag ccgtgaagca gcacgacgac cggcccattg cctccgcctt
      181 tgccatcgct cccaccgacg acgtgtgcgt cgagaccggc cagcgtgatt tttcgcatgc
      241 gcgcacgttg ccgcgcggcg cgtcattctg caacgcccga gcgcggtgaa gcggccgccg
      301 cggtctgatt tgcggttcgg gcccgttcgt ggttgttttt ccgggatgga cttgaaaggc
      361 ctggtcaccg agctcttgtc ggagctcggc aaggtgtcgc agagcgacgc ggtggtcggc
      421 gcagtgcgcg acgcgggcaa agccaaagtg ttgcccctga gccggatcag catcggcttc
      481 ggcacggcga ctggcgaggt gggcggcact tctcaggcca cgggcgcgtc ggaggccacg
      541 gccgctggtg gtggcgccgg cggagccatc gtcgtcgagc cgcgcgcatt cgtggtggtt
      601 ggggaggacg gtgttcctca catgctggcg ctcgagcgcg gcaaaaccgc ggtagcccgc
      661 cccggcgttc gtattttgcc gagtggcgag agcgctcgag agctaacggg cggcgcggcg
      721 ccgcagctcg aagcgccgaa gtcgagcgac ggctgaagcg gccgtgcagc tctggctagt
      781 gctcctcgcg agcgcgctct gtttgggagc gctcgctgca ctcgcggcca tcgagctcga
      841 attcgaagcg cagggcgtcg gagaagcgag cggcgcgtgg gcagccgcgt tcggccttac
      901 gtttgccggt gttttggttt cgggcgtgtt ggcccgggag cagcgactca agctcgaggt
      961 ccgcatcctg ggtcgcaagc tcgatctcca gcgtctgagg aaacgccgcg ctccaaagcc
     1021 cccgaaggaa aagcctcagc agccatccaa gcctcggagg cgccttccca tcggtctctc
     1081 cgaaggcatc gacctcgctc tgctcgagct gcggagaatc gaggtcggtc gggtagacgc
     1141 agcagtgacc tacggttttc gcgatatcgc gctcactggc cggctcgcgg gcgcgttgta
     1201 tgcgctgagc gggatcctgc cgccgacggt gcacttcaac cagaccgtga agtgggacgg
     1261 cgccgaacgc tgggaggcga gcgcttcggg ccgagtagcg ctgtggccgg gacgcgtgct
     1321 cttcgacgtc gtttggttca tgcttcgggc gcgctggcgg cgacgatcac cgaccgagag
     1381 ccgagagccg agtccggagc cgagcgtttt gggaggcagc aagccatgag caagagcaac
     1441 atctccgaaa tcgtcagcag cctgctctcc ggcgtgcacg gcatctcgcg cagcgagacc
     1501 atcatcggtg cgccgcagca agccggcgaa gccacggtga tcccggtgca tcgactgaaa
     1561 atcgcgttcg gcgcggcaag cgcaaccgcg ggcgcgcgcg gtggcaaggt gggcggcgac
     1621 tcgggagggc acggagccgg cggcgccgtc gagctcgagc cggtcgccgc aatcgccgtc
     1681 ggcaaggacg gcgcggcgca cctgctcacg gtcgaaggcg agcagcaaaa cgcctggtcg
     1741 cagctcttgc aagacgcgcc ggatctgctg agcaaggtgc tgcactcggt gggtgaacgc
     1801 atgcggcacg agcttgccgc gcgcggcgcg ccgcccgtac ccgaagtggc gcaggcagaa
     1861 gccgcgaagg ctctgaccga gggcgaaaaa gggcgctagg gggtgtccca agttcctgcc
     1921 cgtcgcgaga gccaagaaac gcgagcgaga agcgcgcccg aggagagagc accggaggaa
     1981 ggctgaagcc tttcgaggag c
//
LOCUS       DASTKS010001087         5762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1511659, whole genome shotgun sequence.
ACCESSION   DASTKS010001087 DASTKS010000000
VERSION     DASTKS010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511659"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..966)
                     /locus_tag="VFQ35_22910"
     CDS             complement(10..966)
                     /locus_tag="VFQ35_22910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HET9933582.1"
                     /translation="MTVQDLLNPKLDLVFKMLFAKANNKELLLSLLNATLKLQGAAIS
                     FELLNPELPKQAVDDKGVVLDVLVRLADGQQVDVEMQAQARAGRRERALFHWARMFSG
                     QLARGGHYHSLKRCLVVFILDFVELSGNNLHSVFSVRDEHRNEKLSDHFELHFLELPK
                     RASAPASRDETMLWAWCKFLCASTEREFQELAMIDPIFQRAKKALEELSRDPEARRQV
                     EDREAALLLYEAEMAMGREEAAAQGRAEGHAQGRAEGHAQGRAEGRGEGRAELLAELL
                     ERKFGPLPAHVLRRVQEARPEELSSWSERLFQASALSDLFTS"
     gene            complement(1149..1364)
                     /gene="tatA"
                     /locus_tag="VFQ35_22915"
     CDS             complement(1149..1364)
                     /gene="tatA"
                     /locus_tag="VFQ35_22915"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9933583.1"
                     /translation="MGSLSPIHWVIVIVVILLLFGPGRLAGVGKGLGEGIRAFKKGLN
                     DDEPKAPEAEPKQLDVKVTETKSETKA"
     gene            complement(1519..1995)
                     /locus_tag="VFQ35_22920"
     CDS             complement(1519..1995)
                     /locus_tag="VFQ35_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933584.1"
                     /translation="MPLAQAEASGCVSTAPERCFDATDDNCNGIIDEGCGIETGPVQF
                     AIAWEPARVDVDLLVSDPAGELAEVGHTLASGLVKQRDCPGRSNECRGQNLENVYLDA
                     PEAPRGTYRVRVVLENLGGQNPPVQVRFGARVGQRTYGMTLRLDQPQGGYETTFTL"
     gene            complement(2159..4819)
                     /locus_tag="VFQ35_22925"
     CDS             complement(2159..4819)
                     /locus_tag="VFQ35_22925"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9933585.1"
                     /translation="MLDPNLPNLPPASASRPPLPQVDLAKWPPDDDGPAFPGPPGGGF
                     PGSYDMGDGNFKKGRFNPVVILIGILAVAGAAAFLLIGFKQDAEKLTIEQGEERKKAI
                     FVLPKNEQIPQWRQWAASEASDELRSEALKQLAWAKDPEGVNLAIKALTYPSDPIQSM
                     AATALAEYGRPMGEPAKDALLAALKTAGKGAKPQIAWALVVLGDTRALTDVLALYRLG
                     HLSTVQRLGGGTAFDPQKIVGLMSLDQLAGMAGDESPAVRQLVATVLSDNAEPKWTDV
                     LIKLVQDKDPDVARQAAPGLGRIGEARAREPLIDAMKQADKDSRKKFLEALRDGIGTE
                     GLVLGLRSVSADDEKLAWYQTKQIFEMINDSKNGQGLNDPRGGNALYQYIQSKPHIHF
                     QWRAAVAMAAIGDVRAVPTLAKRLRMDPLKIYSDQHDWEMELKRDDNERVVASRMIAD
                     LAMLHPDKKQLISEQAEDALIFWIHELPSPHANGLRALAAMESTKDIEALRKWANPNV
                     PLPKEGQQPPMPEEFVVAQSALRYEGWMKDPRAWGVLEKALTARKTQRNEYDITLDGL
                     MQGGVAILGMSLNALATGAADGFNQWGDHKAFKPLLDFVDDVKNNERSRQAACAALAW
                     TAEKEDFLEIAKRIQSYGGAEKSDQFRRGCLLEALIQRPVPGTAPALMQLMTEQSSLE
                     TRNQVARAIAKGGFDQSVEQKLFEMMNNEALMNDAALALILGASPETAARAVAAYANK
                     PKPALDELGELWYRSFGYWSTEDLEAGLIFKFVDNAEAISHVSIRQTPQEWARVMLTR
                     QLDNLLFDNGPHSFTRVVLRYRLWQMAKGNDEAKRNGAIRALKFMKEQGVLLALRDEQ
                     GPVGKLARDAYFELMNPKVVSGVAIPDDSK"
     gene            5139..>5762
                     /gene="mrtC"
                     /locus_tag="VFQ35_22930"
     CDS             5139..>5762
                     /gene="mrtC"
                     /locus_tag="VFQ35_22930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrtC family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9933586.1"
                     /translation="MNIARLLGEDGAAGPLIVAAVTSLVATALSYGLPEQYAATGVGL
                     WFLFVVFVLVLRRGDSATVRRMGLSLGGLFEPEVPIEFPRVLAAGGRALAIGVLLAAV
                     VLPPFWVGYVYWYQPRGAFTPAPWPSLQNDVLGQLLGVAFPEEAFFRGYLQSSLDTAW
                     PPGRRLFGAPVGRSIWVTSAIFAVGHLLTEPYPGRLAVFFPSLLFGFL"
BASE COUNT          877 a         1809 c         1927 g         1149 t
ORIGIN      
        1 accgcgcgct cagctggtga agaggtcgct cagcgcgctt gcttggaaga gccgttcact
       61 ccaagaggac agctcttcag gccgcgcttc ttgcacccga cgcagcacgt gcgccggtag
      121 tgggccgaac ttgcgctcga gtagttcggc gagcagttca gcgcggcctt cgccgcggcc
      181 ttcggcgcgg ccttgagcgt gcccttcggc gcgaccttga gcgtgccctt cggcgcgacc
      241 ttgagccgcg gcctcttctc ggcccatggc catctcggcc tcgtagagca agagagcggc
      301 ttcgcggtct tcgacttggc gtcgggcttc ggggtctctg ctgagctctt cgagagcctt
      361 tttggctcgc tgaaagatgg ggtcgatcat ggcgagctcc tggaattcac gttccgtaga
      421 cgcgcacagg aatttacacc acgcccagag cattgtctcg tcccggctcg ctggtgccga
      481 ggctcgtttg gggagctcga ggaaatggag ctcgaagtga tcactgagtt tttcgttgcg
      541 atgctcgtcg cgaacggaaa ataccgagtg gaggttgttt ccggagagct cgacgaagtc
      601 gaggatgaag acgacgaggc agcgcttgag cgagtggtag tgcccgccgc gagccagctg
      661 tccggagaac atcctcgccc aatgaaacag ggcccgctcg cgccttcccg cgcgcgcctg
      721 cgcctgcatc tcgacatcca cttgttgacc atcggcgagg cggacgagca cgtcgaggac
      781 gactcccttg tcatcgacgg cttgcttggg aagctcgggg ttcaaaagct cgaacgatat
      841 ggcggctccc tgcagcttca gcgtggcgtt cagcagcgag aggaggagct ccttgttgtt
      901 cgccttggcg aacagcattt tgaaaacgag gtcgagcttc ggattcagta gatcttgtac
      961 cgtcatcgag cgagcttccg catcgaccaa cccaatcgcg gatcggtctc gctcacgtcg
     1021 gtcgacgagc ggacgagttg aggaaaatct tgggcggcgc ttctcgaacg agaaccgatg
     1081 cgatcgggat cgaggaagct cttcgcctgg ggctctgcgg cacggcagag ccccaccgag
     1141 gaacgtcgtc aggccttggt ttcggacttg gtctcggtga ccttcacgtc gagctgcttc
     1201 ggctccgcct cgggcgcctt gggctcgtcg tcattgaggc ccttcttgaa ggcacgaatg
     1261 ccttccccga gtcctttgcc gacgccagcg aggcgacccg gtccgaacag gaggaggatg
     1321 acgacgatga cgatgaccca gtgaatcgga cttaggcttc ccatgacgac acgagtccga
     1381 gcgggggcgt atcgcccggg tcggatgcga ctaccataac acccgattcg ccccccgcca
     1441 cctactcggt cagcgcgttt cggccccttc gtgccggtcg ctccaatcgg tggatcgtga
     1501 cgcgacgcgt gtctttcgtc acagtgtgaa ggtcgtttcg tagccgcctt gtggctgatc
     1561 gaggcgcaac gtcatgccgt aggttcgctg tccgacgcgg gcgccaaacc gaacctgtac
     1621 gggtggattt tgaccgccca gattctcgag aacgacgcgc acacggtagg tgccgcgcgg
     1681 cgcctcgggc gcgtcgaggt agacgttctc gaggttctgt ccgcggcact cgttgctgcg
     1741 gccggggcag tcacgctgtt tgacgaggcc gctcgccaac gtgtggccaa cttcggcaag
     1801 ctcgccggcg gggtcgctca cgagcaagtc gacgtcgacg cgcgccggtt cccacgcgat
     1861 cgcgaattga accgggccgg tttcgatccc gcagccctcg tcgatgatgc cgttgcagtt
     1921 gtcgtcggtc gcgtcgaagc agcgctcggg cgccgtgctg acgcagccac tcgcctcggc
     1981 ttgcgcgagc ggcatcgaga gcagcgttcc aggcgccgtg cgcgtggtcg agctgccccg
     2041 tgtcccgcaa cccgaaaatg agagagccgc gagggcgagg cccgaaatca cacccaatcg
     2101 ggtgcgaatc gaacctcgcg tcgcggctct ttggctcact cgaggtcttc cggttggctt
     2161 acttgctgtc gtccggaatc gcgactcccg aaacgacctt ggggttcatg agctcgaagt
     2221 acgcgtcgcg agcgagcttg ccaaccgggc cctgttcgtc acgcagcgcg agcagcacgc
     2281 cttgctcctt catgaacttg agagcacgga tcgcgccgtt gcgcttggct tcgtcgttgc
     2341 ctttggccat ctgccagaga cggtaacgga gcaccacgcg cgtgaaggag tgcggcccgt
     2401 tgtcgaacaa gaggttgtcg agttgacggg tgagcatcac gcgcgcccac tcttgaggag
     2461 tctggcggat gctgacgtgg ctgatggctt cggcgttgtc gacgaacttg aagatgagac
     2521 cagcctcgag atcctccgtc gaccagtacc cgaagcttcg gtaccagagc tcgccgagct
     2581 catcgagcgc gggcttgggc ttgttggcgt aggccgcgac agcgcgggcg gcggtttcgg
     2641 gcgaggcccc gaggatgagg gcgagcgcgg cgtcgttcat caacgcttcg ttgttcatca
     2701 tctcgaagag cttctgctcg accgattgat cgaagccgcc cttggcgatg gcgcgcgcga
     2761 cctggttgcg cgtctcgagc gacgactgct cggtcatgag ctgcatcaac gccggcgccg
     2821 ttcccggaac cggacgctga atgagcgcct cgagcaagca gccgcggcgg aactggtccg
     2881 acttttcggc gccgccgtac gactgaatgc gcttggcgat ctcgaggaag tcttccttct
     2941 ccgccgtcca ggccagagcg gcgcacgctg cctgccgcga acgctcgttg ttcttcacgt
     3001 cgtcgacgaa atcgagcaac ggcttgaagg ccttgtggtc gccccactgg ttgaagccgt
     3061 cggcggcgcc ggtggcgagg gcgttgagcg acatacccag gatcgcgacg ccgccctgca
     3121 tgaggccgtc gagcgtgatg tcgtactcgt tgcgctgcgt cttgcgggcc gtgagcgcct
     3181 tctcgagcac gccccaagcc cgcgggtctt tcatccagcc ttcgtaacgg agtgcgctct
     3241 gcgcgacgac gaactcttcc ggcatcggcg gctgctgacc ttccttcggc agcggcacgt
     3301 tcgggttcgc ccacttgcgc agggcctcga tgtccttcgt cgactccatg gcggcgaggg
     3361 cgcggaggcc gttcgcgtgc ggcgacggga gctcgtggat ccagaagatg agcgcgtctt
     3421 cggcctgctc cgagatgagc tgcttcttgt cggggtgcag catcgccaaa tcggcgatca
     3481 tgcgcgaggc gaccacgcgc tcgttgtcgt cacgcttcag ctccatctcc cagtcgtgct
     3541 ggtcggagta gattttcagc gggtccatgc gcaggcgctt ggcgagcgtc ggcaccgcgc
     3601 gcacgtcgcc gatcgccgcc atcgcgaccg ccgcacgcca ctggaagtgg atgtgcggct
     3661 tgctctggat gtactggtag agagcattgc cgccgcgggg gtcgttcaag ccctggccgt
     3721 tcttcgagtc gttgatcatc tcgaagatct gcttggtctg gtaccaagcg agcttctcgt
     3781 cgtcggcgga aacggagcgg aggccgagca cgaggccttc ggtgccgatg ccgtcgcgca
     3841 gcgcctcgag gaacttcttg cggctgtcct tgtcggcctg cttcatagcg tcgatcaaag
     3901 gctcgcgagc gcgcgcctcg ccgatgcgac cgaggcccgg cgccgcttga cgagcgacgt
     3961 ccggatcctt gtcttgaacg agcttgatga ggacgtcggt ccacttgggc tcggcgttgt
     4021 cgctgagcac ggtggcgacc agttgccgca ccgcggggct ttcgtcgccc gccatgcccg
     4081 ccagctgatc gaggctcatc agcccgacga tcttctgcgg gtcgaaggcg gtgccgccgc
     4141 cgaggcgttg cacggtcgac aagtgaccca agcggtagag cgcgagcacg tccgtcagcg
     4201 cgcgcgtgtc gccgagcacg acgagcgccc aggcgatctg cggcttggcg cctttaccag
     4261 cggtcttcag ggcggcgagc agcgcatcct tcgccggctc gcccatcgga cggccgtact
     4321 cggcaagcgc ggtcgcagcc atcgactgga tcgggtccga cggataggtg agcgccttga
     4381 tggcgaggtt cacgccctct ggatccttcg cccaggcgag ctgcttgagc gcttcggagc
     4441 gaagctcgtc gctcgcttcg ctagccgccc actggcgcca ctgcgggatc tgctcgttct
     4501 tcggtaagac gaagatcgcc ttcttgcgct cttcgccctg ctcgatcgtg agcttctcgg
     4561 cgtcttgctt gaagccgatc agcaggaagg ctgctgcgcc agcgaccgcg aggatgccga
     4621 tcaaaatcac gaccgggttg aagcggcctt tcttgaagtt gccgtcgccc atgtcgtagc
     4681 tacccggaaa gccgccgccg ggcggaccgg ggaacgccgg gccgtcgtca tctggcggcc
     4741 acttggcgag atcgacttgc ggtaatggcg ggcgcgaggc agaagccggg gggaggttcg
     4801 gcaggttcgg gtcgagcatg agtccttccg cgagcgctgg tcagacgcat cgaaagcacc
     4861 acgcggcctt cgactgtctg gaaacgtgcg cggcgacctt aacactagtc aggctggatt
     4921 tcgcgccttc tgtaccccgc accgacgatc taccgatggc gtcgaaaaca cctttacttg
     4981 ggtgcggtga gctgccgacg gcaccgaaac cgcatgtctc agcgcgtcgc gttgcgatgc
     5041 gaggtgaact ttgcattcga gttgatcgat gcgctccgcg gccgtcgggc cagggcagcg
     5101 gcgttcagaa gtgtttggcg tggcgtgggc ggtagcgcgt gaacatcgct cgactccttg
     5161 gcgaagacgg cgcagcgggg ccgctgatcg tggcggcagt gacctcgctg gtcgctaccg
     5221 cgctgtccta cggtctcccg gagcaatacg cggcgaccgg cgtcggtctc tggttcttgt
     5281 tcgtcgtgtt cgtgctcgtg ctgcgtcgcg gcgatagcgc gacggttcgg cgcatggggc
     5341 tctctctcgg cggtttgttc gagccggagg tgcccatcga gtttccgcga gtgctcgcgg
     5401 cgggagggcg cgctctggcg atcggcgtgt tgcttgcggc cgtcgtgctg ccgccgtttt
     5461 gggtgggtta cgtctattgg taccaaccgc gtggcgcttt cacgcccgcg ccgtggccat
     5521 cgttgcagaa cgacgtgctc ggtcagttgt tgggggtcgc gtttcccgag gaagcgttct
     5581 tccgcggtta tctacagagc tctctcgaca cggcttggcc cccggggcgg cggctgttcg
     5641 gcgcccccgt cgggcggtcc atttgggtga cgagcgccat cttcgcggtt ggtcacttgc
     5701 tcaccgaacc atacccaggg cggctcgcgg tcttctttcc gtcgctgctc ttcggcttct
     5761 tg
//
LOCUS       DASTKS010001088         5447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_616335, whole genome shotgun sequence.
ACCESSION   DASTKS010001088 DASTKS010000000
VERSION     DASTKS010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616335"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFQ35_22935"
     CDS             <1..292
                     /locus_tag="VFQ35_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933587.1"
                     /translation="GVEYAINVQDRARFVDGVRNAGRSVGGAVALCSWTTIIGYGALW
                     FADNLALQSFGHYAIIGEVACLGAALIVLPAALTVLSSRPKAFLAEPNGSQG"
     gene            complement(335..580)
                     /locus_tag="VFQ35_22940"
     CDS             complement(335..580)
                     /locus_tag="VFQ35_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933588.1"
                     /translation="MRLSADEATQRGVSIRVYPGASRDAILVKAPVVLHPKYEYVKFS
                     IPALPGVEDFQLELNAKVHERAIGQIVTERCDQSCSL"
     gene            complement(888..1607)
                     /locus_tag="VFQ35_22945"
     CDS             complement(888..1607)
                     /locus_tag="VFQ35_22945"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET9933589.1"
                     /translation="MTPELDPHHADELLVEIETLRGEDAGAALRKLEAAPPEVTRRAE
                     ARLLAADLTWEVAGPDAALPLVEALVHDVPDYADARYLLGSLYDELGKDRQMVEEFLE
                     VLRLDEGLDSGLDAEEGRELEDLIVEAAEDTIAGLPQAFRERLQGVPVLVAARPDEEL
                     VREGFDPRALGLFEGPTDAEHQNNDAVGLPTRIVLFSANLVAHSVDEEQLKSEVATTV
                     LHELGHYFGLEEDDMERLGLD"
     gene            complement(1604..1732)
                     /locus_tag="VFQ35_22950"
     CDS             complement(1604..1732)
                     /locus_tag="VFQ35_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933590.1"
                     /translation="MGYPGLILEGRVAPVGGGTAPPLVNGQFIAARGGFLASGVNQ"
     gene            1950..4301
                     /locus_tag="VFQ35_22955"
     CDS             1950..4301
                     /locus_tag="VFQ35_22955"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9933591.1"
                     /translation="MPIFAVSLVSSRALAGLLRRIVRVEAALVPVLCSLLFWSPDARA
                     RDSSGRVHVTYWEKWRGVEMDAMKVLVDDFNRSQSRIEVEYIPTSSVSKKTLVATAGG
                     EPPDLAGLVAQDVFDFAEKGALIALDDWARGTVVDAARYVPAYWDMGVARGRLYGVVS
                     VPAVNALHWNKRLFRAAGLDPERPPRTIAELDGYAKRLTRVEDGRIVQAGFLPSDPTW
                     WAFFWPYFFHGRLWDGEARVTLTEPENVQAFTWVQSYAKEHGVLALQNLSATFGNTAS
                     AQDPFLSEKLAMVLQGVWLANFVDAFAPNLDWGAAPFPSIDADDPPVSFVDADMLVIP
                     RGAAHPREAFEFIRFLSERANTEKLCLLQRKNSPLMDVSASFFEKHKNPYIRMFQALP
                     SSPGARGPLKMSVWPEYRTELHATFQKVWLARATPEQALAETNARMQRSLDRARRREA
                     TPPSPSLLDVPLWLVGALVAVLSLIVAREFVKRRRLGASSRAELRALGKGLAFSSPWV
                     VGLLSFVAYPVLSSIVYSLCDYSLLTAPRFVGLGNYLDLLHDDLFFISLKNTAIYALF
                     AVPLHLLAAFSLALFLDANLRGSGVYRTLLFLPSLTPIVASSMAWLWIFNPRFGVLNY
                     ALGKLSFGLFPPVPWLSNPNTALPSLIMMSVWGIGHTIVVTLAAMQDVPSAVYEAADI
                     DGANLWQRVWHITLPLTSPVMYFNGVMGLITALQVFTQPYIMTQGGPARATLSYSMRL
                     YDNAFTYLRMGYASAMAWILFVIILGLTVFALRIGKNRMHYTS"
     gene            4309..5175
                     /locus_tag="VFQ35_22960"
     CDS             4309..5175
                     /locus_tag="VFQ35_22960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9933592.1"
                     /translation="MSAAGSPFSSTFRRHPASKLLVHAVLIAASLMFCFPMLWMVSTS
                     LKPIEQAMAYPPRLIPDALEWRNYAETVRYVPFWSYTVNTLTISLLSAAGTTLSSALV
                     AYSFTRLEWAGRNAFFALTLATMMVPFPVLMVPLYTVFRALGWIGTLKPLWVPAFFGS
                     AFNIFLLRQFFTRIPKSLSEAMTLDGASELTIFLRIYLPLSRAPLAVAAFFQFIYSWN
                     DFLGPLLYLTNQETFTLSLGLQAYQSQHGGTQWHYLMAASFLTTLPIVVLFFFMQRSF
                     LAGMSFMKNVDA"
     gene            5227..>5447
                     /locus_tag="VFQ35_22965"
     CDS             5227..>5447
                     /locus_tag="VFQ35_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933593.1"
                     /translation="MALVKLLLDVGTDLAELVGQTLIELGAGGVEEQAGERGARLIVY
                     GDDAEQLSALAEQARSALGEMGIDEDEGNL"
BASE COUNT          873 a         1764 c         1685 g         1125 t
ORIGIN      
        1 cggggtcgag tacgcgatca acgttcaaga ccgcgctcgc ttcgtcgacg gcgttcggaa
       61 tgctggaaga tccgtgggcg gcgccgtagc gctctgcagc tggacgacca tcatcggcta
      121 cggcgctctc tggtttgcgg acaacctagc gcttcaatcg ttcggccatt acgccatcat
      181 cggcgaggtg gcttgtctcg gggcggcgct catcgtgtta cccgccgcct tgacggttct
      241 ctcgtcccgc ccaaaagcgt tcttggccga accgaacggc agccaggggt gatgttcggc
      301 ggaacgcgac cctcgtcgag gcgcatcgag ctgctcaaag cgagcagctc tggtcgcagc
      361 gctcggtaac gatttgtccg atcgcgcgct cgtggacctt cgcgttgagc tcgagttgaa
      421 aatcttccac gccgggcagc gccgggatac tgaacttcac gtactcgtac ttcgggtgca
      481 gcacgaccgg cgctttcacc aaaatggcgt cacggctcgc gcccggatac acgcggatcg
      541 aaacgccgcg ctgcgttgct tcgtcagccg agagcctcat cgcgaactcg gcttgcgagc
      601 ctctgcgcga caggcagtaa catcggcgcg caacgtgaat gccttccgga gcgggggccg
      661 atggattcgc gatcggcccc ggacgcttta ccgggcgcgc gacttctgga gcgggttgcc
      721 gagcgctccc tgcgcaaccg aacgaagcga atgaaacggc gccgatgaag caaagtgctg
      781 accgcgacga gcgcagcatg tcggttactt acacccgccc gtcgtcggag tgtcactgtt
      841 tcggtttcgc cgaagcgcta cactcgtcgt gaactttgcc gagcggctca gtcgaggccg
      901 aggcgctcca tgtcgtcttc ttccagcccg aagtagtgac cgagctcgtg cagtacggtg
      961 gtcgcgacct cgctcttcag ctgctcttcg tcgaccgagt gcgccacgag gttcgcggaa
     1021 aacagcacga tccgagttgg cagtcccacc gcgtcgttgt tttgatgctc tgcgtcggtg
     1081 gggccttcga ataggccgag cgcacgtgga tcgaaccctt cgcgcaccag ctcttcgtcg
     1141 ggccgagccg cgaccaggac gggcacccct tggagccgtt cgcgaaatgc ttgcggaagg
     1201 cccgcgatcg tatcctccgc ggcttcgacg atgagatctt cgagctcgcg accctcttcg
     1261 gcgtcgagcc cggagtcgag gccctcgtcg aggcgcagca cctcgaggaa ctcttcgacc
     1321 atctgacgat ccttaccgag ctcgtcgtac agagaaccga gcaagtagcg cgcgtccgcg
     1381 tagtcgggga cgtcgtgaac gagggcttct accaatggca gagcggcgtc ggggccagct
     1441 acctcccacg tgagatcggc tgcgagcagg cgcgcctcgg cccgacgcgt cacttcgggc
     1501 ggggcggctt ccagcttgcg gagcgcggcg cccgcgtctt ccccacgcaa agtctcgatt
     1561 tcgaccagca gttcgtcagc gtggtgggga tcgagctcgg gagtcactgg ttcacgccgc
     1621 ttgccaggaa gccgcctcgg gcggcgatga attgcccatt cacgagcggt ggggcggttc
     1681 cgccgcccac gggcgcgacg cggccctcca gtatcagtcc cgggtatccc atgtttgctc
     1741 ttcttacgac gtccggcgct ggtgtagcag atgccggcgg ctcggctgcc aagatcccga
     1801 tgcttggggt ccatggcgtt cggcgccccc cggccgaaac gcggatactc gttgagttgc
     1861 agctcagaac gaagggatcc cctggtccga cccggcttga cccattcgtg agcaaactcc
     1921 tatggtgagg cgctctccat ccctgctcga tgccgatttt cgctgtctcg ctcgtgagct
     1981 cgcgagccct cgcagggctt ctccgacgca tcgtcagggt cgaggcggcg ctcgttccgg
     2041 tgttgtgctc gctcctcttc tggagcccgg acgcgcgtgc gcgggatagc tcggggcgag
     2101 ttcacgtaac gtactgggaa aaatggcgtg gcgtcgagat ggacgccatg aaggtcctcg
     2161 tcgacgactt caaccgctcg cagtcgcgaa tcgaggtcga atacattccg acttcgagcg
     2221 tgagcaagaa gactttggtt gcgaccgctg ggggagaacc cccggatcta gcggggctcg
     2281 tcgcgcaaga cgtcttcgat ttcgccgaga aaggtgcgct catcgcgctc gatgattggg
     2341 cgcgcggcac cgtcgtcgac gccgcccgct acgtgcctgc gtactgggac atgggcgtcg
     2401 ctcgggggcg gctctacgga gtcgtctccg tgccggccgt caatgcgttg cactggaaca
     2461 agcgcctgtt ccgcgcggcg gggctcgatc cggaacggcc gccccgcacc attgcggagc
     2521 tcgacggtta cgccaagcgt ctcactcggg tcgaggacgg gcgcatcgtc caagccggtt
     2581 ttttgccttc ggatcccacc tggtgggcgt tcttctggcc gtacttcttt cacgggcggc
     2641 tctgggacgg cgaggcgcgc gtcaccttga ccgagccgga gaacgttcag gccttcacct
     2701 gggtgcagag ctacgccaag gagcacggcg tgctcgccct gcagaacctt tctgcaacgt
     2761 tcgggaacac ggcgtcggcg caagacccct tcttgagcga aaagctcgcg atggtgttgc
     2821 agggggtttg gctcgcgaat ttcgtcgatg ccttcgcgcc gaacctggat tggggggcgg
     2881 ccccgtttcc gagcatcgac gcggacgacc ctccggtttc cttcgtcgac gcggacatgt
     2941 tggtcatccc gcgcggcgcg gctcacccgc gtgaagcgtt cgagttcatc cgtttcctct
     3001 ccgagcgggc caacaccgag aagctgtgtt tgttgcagcg caagaactcg cccctcatgg
     3061 acgtgagcgc gagcttcttc gagaagcaca aaaatcccta cattcggatg tttcaagcgc
     3121 tgccgtcgag cccgggcgcg cgcggcccgc tgaagatgag cgtttggccc gagtatcgaa
     3181 ccgagcttca cgccacgttc cagaaagtct ggctcgcacg cgcaacgccg gagcaagcgc
     3241 tcgcggagac gaacgcgcgc atgcaacgct cgctcgaccg tgctcgtagg cgtgaggcga
     3301 cgcccccctc cccatcgctg ctcgacgtgc ctctctggct agtcggcgcg ctcgtcgcgg
     3361 tgctttcgct catcgtcgcg cgcgagttcg tcaaacgccg acgcctcggt gcttcttcgc
     3421 gagcggagct cagggcactc ggcaaggggc tcgccttctc gtccccttgg gtggtggggc
     3481 tgctctcgtt cgtggcgtat ccggtgcttt cgagcatcgt ctatagcttg tgcgactact
     3541 cgctcctcac cgcgccgcgc ttcgtgggcc tcgggaacta cctcgacttg ctccacgacg
     3601 atttgttctt catctccctg aaaaataccg cgatttacgc gctcttcgcg gtgccgctcc
     3661 acctccttgc cgcgttctcg ctggcgctct tcctcgacgc gaacctgaga ggctccggtg
     3721 tttaccgcac gctcctcttc ttgccgtcgc tgacgccaat cgtcgcaagc tcgatggcct
     3781 ggctctggat cttcaaccct cgcttcggcg tgctcaacta cgcgctcggc aagctgagct
     3841 tcgggctctt cccgccggtt ccctggctga gcaacccgaa caccgctctg ccgtcgctca
     3901 tcatgatgag cgtctgggga atcggtcaca cgatcgtcgt cacgctcgcg gccatgcagg
     3961 acgtaccgag cgcggtgtac gaagcggccg acatcgacgg tgcgaacttg tggcagcgcg
     4021 tctggcacat cacgttgccg ctcacttcgc cggtgatgta cttcaacggc gtgatggggc
     4081 tcatcacggc gctccaggtg ttcacgcagc cgtacatcat gacccaaggc gggccggcgc
     4141 gcgcaaccct cagctactcg atgcgcctct atgacaacgc cttcacgtat ttgcgtatgg
     4201 gctacgcctc cgcgatggcg tggattctct tcgtgatcat cctcgggttg acggtgtttg
     4261 ccttgaggat cggcaaaaat cgcatgcact acacctcgtg agcgaagcat gagcgccgcc
     4321 ggttcaccct tcagcagcac ttttcggcga catcccgcgt cgaagctact ggttcacgcc
     4381 gtgctcatcg ccgcgagcct gatgttttgc tttccgatgc tgtggatggt gtcgacgtcg
     4441 ctcaagccga tcgaacaagc gatggcgtat ccgccgcgtc tcatccccga tgcgctcgag
     4501 tggcgaaact acgccgagac ggtgcggtac gtcccgtttt ggtcctacac ggtgaacacg
     4561 ctcaccatct cgctgctttc ggcggctgga accaccctgt cgagcgctct cgttgcttac
     4621 agcttcactc gcctcgagtg ggccgggcgc aacgctttct tcgcgctcac tctggcaacc
     4681 atgatggtgc cttttccggt cctgatggtc ccgctctaca ccgtctttcg tgcgctcggg
     4741 tggatcggaa ccttgaagcc gctgtgggta cctgcgttct tcggcagcgc attcaacata
     4801 ttcttgctgc gacagttctt cacgcgcatc ccgaagagct tgtcggaggc gatgacgctc
     4861 gacggggcga gcgagctcac gatctttctt cggatctatc tgccgctctc gcgtgcgccg
     4921 ctcgcggtgg cggcgttctt ccagttcatc tattcctgga acgactttct cggcccgttg
     4981 ctctatctga cgaatcagga aacgttcacg ctgtcactcg ggctacaggc ctaccagagt
     5041 cagcacggtg gcacgcagtg gcactacctc atggcggcgt cgtttttgac gacgcttccc
     5101 atcgtggttc tcttcttttt catgcagcgt tccttcttgg ctggtatgag cttcatgaag
     5161 aacgtcgatg cttgacgccg ccctcggcgg gtcgaaactc ggaaccttag cagagggaaa
     5221 agaatcatgg cgctcgtcaa gttgctgttg gacgtcggca ccgacctcgc cgagctcgtt
     5281 gggcaaacgc tgatcgagct cggtgcaggc ggcgtcgaag aacaagcggg cgagcgcggc
     5341 gcacgcctca tcgtgtacgg cgacgacgcg gagcagcttt cggcgctcgc cgaacaagcg
     5401 cgctctgcgc tcggcgaaat gggtatcgac gaagacgaag gcaatct
//
LOCUS       DASTKS010001089         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1958794, whole genome shotgun sequence.
ACCESSION   DASTKS010001089 DASTKS010000000
VERSION     DASTKS010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958794"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..611)
                     /locus_tag="VFQ35_22970"
     CDS             complement(96..611)
                     /locus_tag="VFQ35_22970"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9933594.1"
                     /translation="MSLARYCEGKRVIVRNSHTSAFEGVRALADNHVGALIVQEEGRL
                     VGIVTDRDLALRVIGFELNPKTTRLRDVMSPEPVTLSPEDSEEQAFKLMRARHVRRIP
                     IVDEGKVVGMVTLDDLLMSGAIDASTAAQIVDEQLSEPSLAKAAGQVYPTLASALHAV
                     TSPGENERSDE"
     gene            complement(823..1536)
                     /locus_tag="VFQ35_22975"
     CDS             complement(823..1536)
                     /locus_tag="VFQ35_22975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933595.1"
                     /translation="MGQRIAPRRVNLRMQALGDARVISLVVSGLHLVDRIARQLSRRL
                     GAWLEYEELLSSGREGLFDAARRYEHARGAPFAAYASLRIRGAMLDSARRMAGLPRRT
                     YERALAETPQASDDGETPTSCPRLKRPTDDGSEVALAARTATLTMVSDLKAFEESRKL
                     AVTQDPESALERAELLALIRVGISALRGDEAELVHRHYFEDERLDDIAASLGITASWA
                     SRILSRAVARLTRQMRRKA"
     gene            complement(1766..2503)
                     /locus_tag="VFQ35_22980"
     CDS             complement(1766..2503)
                     /locus_tag="VFQ35_22980"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9933596.1"
                     /translation="MAIGVVSDIHGNLFALRAVVDDMVRRGVSRVINLGDNLSGPLLP
                     RETGEYLRAQAWLHLAGNHERQILESRPTAVYNSDAYAHERLTPELLSWLRELRPVVE
                     LDADLLCCHGTPRSDSEYLLETVTPEGIRLATTDEIEERLEGNRHALVLCGHSHVPRA
                     VRHGGSLLVNPGSVGLQAYFAKGPPSYIVETGSTDARYAIVERLETGWRAELISVPYD
                     YSAAVALAAHVSREWAHALGTGRAFTG"
BASE COUNT          464 a          893 c          822 g          457 t
ORIGIN      
        1 caaggcctac ttcacaaggg attcaccggg ctctgagcgc tcgctgcgga agccgagtcg
       61 ccgggacgcc gtcgagccca agcgctcggc ccgcttcact cgtccgagcg ctcgttttct
      121 cccggggaag tgacggcgtg cagcgccgag gcgagcgtcg ggtaaacttg gccggcagcc
      181 ttcgctaacg acggctccga tagctgctca tcgacgattt gcgcggcggt ggaggcgtcg
      241 attgcgcccg acatcaacag atcgtcgagc gtcaccattc ccacgacttt cccctcgtcg
      301 acgatcggaa tgcgccggac gtgcctcgcc cgcatcagct tgaacgcttg ctcttcggag
      361 tcctcggggc tcaaggtcac cggttccggg ctcatcacgt cgcggaggcg cgtcgtcttc
      421 gggttcagct cgaagccaat cacccgcagc gccaagtctc ggtcggtaac gatcccaacc
      481 aggcgccctt cctcttggac gatgagcgcg cctacgtgat tgtccgccag ggctcgcacc
      541 ccttcgaacg cagacgtatg cgagttgcga acgatgacgc gcttaccttc gcaatatcga
      601 gccaacgaca tgcgttaact atgggcaaac caaacgccaa acgcatcaga atttcggaga
      661 atctcggcgg ggcatgctcg tgaccgcaat gtccctggcg gagctcgagc agaactgccg
      721 cgctccgcag cagcgcctct tcaatgcaga ccccgctcgt accagcgaac gcccgagcgg
      781 acgccgtcac tcaccaatcc cgccaccgta tggaagcttg cttcaagctt tgcgtcgcat
      841 ttgacgggtc aggcgcgcga cggcccgaga taagatgcga ctcgcccagg acgcggtgat
      901 gccaaggctc gcggcgatgt cgtcgagcct ttcgtcttcg aagtagtgtc ggtggacgag
      961 ctcagcctcg tcgccacgga gcgcgctgat gccgactcgt atcaaggcga ggagctccgc
     1021 gcgctcgaga gcactctcgg ggtcctgagt taccgcgagc tttcttgatt cctcgaacgc
     1081 cttcaagtcc gaaaccatcg tcaaagtcgc ggtgcgagcc gccagcgcga cctcgctgcc
     1141 gtcatcggtc gggcgcttga gtcgcgggca ggaagtcggc gtctcgccat catccgaagc
     1201 ttggggcgtt tcggccaacg cgcgctcgta ggttcgccga ggaagaccgg ccatgcgtcg
     1261 cgccgaatcc aacatcgccc cccgaatccg caggctcgcg tacgcggcga acggcgcgcc
     1321 gcgcgcgtgc tcgtagcggc gagccgcatc gaagagcccc tcccgccccg agctcagcag
     1381 ctcttcgtat tcgagccaag caccgaggcg ccgggaaagc tggcgcgcga tgcggtcgac
     1441 caggtggagc ccactgacca ccagggagat gacgcgggcg tctcccaaag cctgcatgcg
     1501 caagttcacg cggcgcggcg cgattcgttg acccatctcg ccgggcattt tgcacgagtc
     1561 acaaccctcg agcgagtagt gaaatcaccc ctcgtgtaaa taggggatgt tcctaccctt
     1621 ttgtatatcg ccggcgggct cggccccggc gcccgggctc gacgcgcagc ccaaacgcac
     1681 gccgaacctc accgagacag cgccgatgct caccccgctc ggacggcgcg aacgccgtgc
     1741 gctcgcctcc ctcgcccttt agccgttagc ctgtgaatgc gcggccagta cccaacgcgt
     1801 gcgcccactc ccgggacacg tgcgctgcga gcgcaaccgc cgccgagtag tcgtacggaa
     1861 cgctgatgag ctccgcgcgc cacccggttt cgagtcgctc tacgatcgcg taacgcgcgt
     1921 cggtggaccc cgtctcgacg atgtacgaag gtgggccttt cgcgaaatag gcttgcagcc
     1981 cgacgctgcc gggattcacc aatagactgc ccccgtgtcg caccgcccgc ggcacgtggg
     2041 aatgcccgca cagaacgagc gcgtggcgat tgccttcgag ccgctcttcg atctcgtcgg
     2101 tggtcgcgag gcgtatgccc tcgggagtca ccgtctcgag caagtattcg gagtcgctgc
     2161 gcggggttcc gtggcagcac aagagatctg cgtctagctc gaccacgggt cgtagctccc
     2221 gtaaccaact caataactct ggggtgagcc gctcgtgcgc atacgcatcg gagttgtaga
     2281 cggcggtcgg tcggctctcg aggatctgcc gttcgtgatt accggcgagg tgcagccacg
     2341 cctgcgcgcg caggtattcc ccggtttcgc gcggcaagag tggccctgac aagttgtcgc
     2401 ccaagttgat tactcgcgag acaccgcggc gcaccatgtc gtcgacgacg gcgcggagcg
     2461 cgaacagatt gccgtgaatg tcggagacga cgccgatcgc catgagtaat ccgattctga
     2521 cacgggcccg ccgcgctgtc cgcagggcag cgaccggcgg gacgttgccc cgaatcgcga
     2581 gtcggggcgt gacagcgccg tcaacgtgag gcactttgcg cgtgctgatt gacctc
//
LOCUS       DASTKS010001090         7830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_616672, whole genome shotgun sequence.
ACCESSION   DASTKS010001090 DASTKS010000000
VERSION     DASTKS010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616672"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..2298
                     /locus_tag="VFQ35_22985"
     CDS             289..2298
                     /locus_tag="VFQ35_22985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933597.1"
                     /translation="MVDRFSTPPASLSGMVIHSELQAGVSYRIEKPIGEGGLGTAYLA
                     LRQAEDGTSPVVVKVVRGSPVVGNISPDILVRKEAVALGRLNERIPPTPFVVRFIDTG
                     TARLFDETPMPWLALEYVHGGLEGTTLEDRVAYSINRTSFAFDPNRTAHLLRCMAAGL
                     SAIHAVGVVHRDITPGNVLCCGFGEGEIFKISDFGVARPQGLTATFGGMGVGTAGYAA
                     PEQSLPETSAAGPFTDVFGLACVLFYVMTGEHYFDCSSPVQAYSLVKDRRRRSITECR
                     TLSPEFLERPDVCRQVDITLARATAFSAEQRHQTAQELANALIPWLGDPTSAPRPSRR
                     LVNSLLNLSPPGDLSKWTWTTRHPPGDEVVIQSAAWDADGRCFVFTPRGPLFWNGQSW
                     LDAPEVEHNLPAGMTFAKRYEAGGWLVGGSGGALAVYNTYGVREVVRAPDVTLEFSHA
                     SGRFDDLVVAVGQRPDEAPSLWAMAARRWMKPLRLDGVSYVAALHRLDESRWLLCGRL
                     TQGIGFAAIYTPMQWEVEYLDVPHTRAFVSGASEPERGVALLVGADGVALRVGATATT
                     STLANGSPDLTAAAMDVLAREWVASLGALWVRDAEREMEWQRVWSAPEWKTPFISMMA
                     DAGMVVAMTVDGGIVEGRAAWHALRGRVSSGPPRARDSGSSFPPR"
     gene            2398..3588
                     /locus_tag="VFQ35_22990"
     CDS             2398..3588
                     /locus_tag="VFQ35_22990"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9933598.1"
                     /translation="MRTWLLGGLSCAILSAACSSDTSHSGGSGGAANGGSTANSGGSV
                     AAGGSGAGGKTTGAGGAVNSGGITSNSGGANAGTSSRGGSAGGGGTSAGGGAPANGGT
                     NAGGKASGGASAGGRASGGGGGGGKSSGGSAGAAGASTGTGGGGATVGCSASATGYKL
                     VWNDEFEGPAGSAVDAAKWTHDIGGNGWGNNELEYYTDGAANAALDGSGNLVITAKKQ
                     TMSGKDYTSARLKTQGLYSFTYGRVESRMKIPSGQGMWPAFWMLGSDIDKNSWPDCGE
                     IDIMENIGKEPNIIHATIHGPGYSAGAGPTKQYSLPDPVASAFHVFAIEWEEGAIRWY
                     VDDMLYSTKTKADIPSSATWVYSHPFFLLLNVAVGGGWPGNPDSTTMFPQTMTVDYVR
                     VCQK"
     gene            complement(3824..4420)
                     /locus_tag="VFQ35_22995"
     CDS             complement(3824..4420)
                     /locus_tag="VFQ35_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933599.1"
                     /translation="MKLIDSCLALIACVPLVVSACGGTQPSPEAAPPVPAAPASASAA
                     PPVAPPPASAAPAASAAPAPGTTPEASAAPSAAKPAINWDAMDKTQRLEHMKTVVMPR
                     MKELFASVDAKRYGDMKCVTCHGDGAKNQTFKMPNPKLPKLSYTDSFKKHMQKTPEIT
                     KFMMEKVVPEMASLLGEQPYDPATHKGFGCAECHLMGP"
     gene            complement(4491..5864)
                     /locus_tag="VFQ35_23000"
     CDS             complement(4491..5864)
                     /locus_tag="VFQ35_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933600.1"
                     /translation="MNSPPNPANDVWARIDAVVTATRPPRNYEAFLERAATSHEDYLF
                     AARVVRFGLRDTDRLLPPRDLTVRATPGGVELASRTLGGALEVDGVKEPAAEALLALF
                     NGERTLADARREATGAQTELERLVSAAFARMLFAPFSLTELEGRISGTEVTRFVGTPY
                     EIDRRYWENMRDVRELSAHALDGANDTQSFVRALQRLHVVALMGASLDNFYRPASRTS
                     SGVQPGALYVTEVRSEVRDGETILLAGPRVGVPFVGGRIYHALVCAHDPAALEDAREV
                     IDERGLSWGRIVRGRASHETESRAWFLPPRPLQPAHLDRLYAAYVDARDAAEHEDVER
                     LRRALSHFHFRFVHLHPFRCANQSLCMNLVNRLLLQGAGAAMPHGLLDQFALRLDEAA
                     YEVVFARAAKRAISTSNAAERWSALQRETTEVYALIQRLQQSPDLEAARAVCEAAPHA
                     AEAALIA"
     gene            5952..7061
                     /locus_tag="VFQ35_23005"
     CDS             5952..7061
                     /locus_tag="VFQ35_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933601.1"
                     /translation="MTLCGGFPGLALVVLGANAAAPVPAPMPTPVEVELEAPSGCADS
                     RSFFERLRARTDRVRLAQPGERALIIDVRVSRVGAKVHGELRFRQDATESATRQVDGA
                     TCEEVVGVFTLTAALALERLNAESESGNAALAIDGVDGQGGASSAGSAKAQAGAPSAG
                     SGGAAAGAGNGNGKTVSEGSGSDDEPPAETWETDPMGGLLRRTIHVELGAGVGLTQVL
                     SPLLATGVGPLVRVRHRRMAVGLSSLYWSSRLTNADTSTSLRSLGFSLSACPWSWQAE
                     RVFFEPCVLGNVGFLHVSNSSVVNSRAADRTLWGIGVLGRVRAAISPVLALELEGGFH
                     VPLVERRFITERPERIVAETPWISPFVGLAAVVSP"
     gene            7254..7757
                     /locus_tag="VFQ35_23010"
     CDS             7254..7757
                     /locus_tag="VFQ35_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933602.1"
                     /translation="MVDSYIDFVARVLRNAGTPDADVDDAVQRTFITAANRLADIRVG
                     AEKSFLLQIALHIAAHARRTVARRREVHADEAPEQVEAFATPEQLTDQKRARQMLDRV
                     LDQMDADLRTVFVLYEFEELNMAEIATALRIPQGTVASRLRRARADFRQRVARLEGTS
                     GTRWDDE"
BASE COUNT         1282 a         2503 c         2669 g         1376 t
ORIGIN      
        1 caggcgcgcg cggcgtccac gtgcgcggcg gcgttgaagt ggcggtaatg atgctgaatg
       61 aagtcgcgga ccgtacccct ggtgatgctc ctcgcggttc cgggggacgc ttttccgcgt
      121 ttccccagca aagtcaagcg agcggcgccc gccgaacgag cgcgacctcg aaacttacgg
      181 cgcttttgtt cggaacgagg cgcgctggca cgcgacgact ggtctgtgac aattccgtgc
      241 taggctcggc ggcgtttttt cgggttcttc gggcgcagcg gaattccgat ggttgatcgc
      301 ttctcgacgc ctcccgcttc tttgagcggg atggtcattc actccgagct ccaagccggc
      361 gtcagctatc gcatcgaaaa gccaattggc gaaggcggtc tcggcaccgc gtacctcgcg
      421 ctgcgccaag cggaagacgg cacgtcaccg gtcgtcgtca aggtggtccg cggctcaccc
      481 gtcgtcggaa acatcagccc ggacattctg gtgagaaaag aagcggtcgc gctcggccgc
      541 ttgaacgagc gcatcccccc gacgccgttc gtcgtgcgtt tcatcgacac cggtaccgcg
      601 cggctgttcg acgaaacgcc gatgccttgg ctggccctcg agtacgtgca cggcggtctc
      661 gagggtacga ccctcgaaga tcgcgtggcg tactcgatca atcgcacgag cttcgccttc
      721 gatcccaatc gcacggcgca cctcttgcgc tgcatggcgg cgggcctctc ggccattcac
      781 gccgtcggcg tggtgcatcg cgacatcacg cccggcaacg tcctctgctg tggcttcggc
      841 gagggcgaga tcttcaagat ctccgatttt ggcgtcgcgc gtccgcaggg cctcaccgcg
      901 acgttcggcg ggatgggcgt tggcactgcc ggctacgccg cgcccgagca gagcttgccc
      961 gagacgagcg ctgccggtcc gttcacggac gtgttcggct tggcttgcgt cctcttttac
     1021 gtgatgacgg gcgagcacta cttcgattgc tcttctcccg tgcaggccta ttcgttggtg
     1081 aaagataggc gacgtcgcag catcaccgag tgccgcacgt tgagccccga atttctggag
     1141 cgccccgacg tgtgccgtca ggtcgacatc acgctcgcgc gcgcgaccgc tttctctgcc
     1201 gagcaacgcc accaaaccgc gcaagagctc gcgaatgctt tgattccgtg gctcggcgat
     1261 ccgacttcgg cgccgcgccc cagtcgtcgc ctcgtgaaca gcctcttgaa cctctcgccc
     1321 cccggcgatt tgagcaagtg gacgtggacg acgcggcatc cccccggcga cgaagtcgtg
     1381 attcagagcg cggcctggga cgccgacggg cgctgcttcg tcttcactcc gcggggcccc
     1441 ttgttctgga acgggcaatc gtggctcgac gcgcccgagg tcgagcacaa cttgcccgcg
     1501 ggcatgacgt tcgcgaagcg ctacgaggca gggggctggc tcgtcggagg ctccggcggc
     1561 gcgctcgccg tttacaacac ctacggcgtg cgcgaagtcg tgcgagctcc ggacgtgacg
     1621 ctcgagttct cgcacgcgag tgggcgcttc gacgacttgg tggtcgccgt tggtcagcgc
     1681 ccggacgaag cgccttcgct gtgggcgatg gcggctcgcc gctggatgaa accgcttcgc
     1741 ctcgacggag tgagctacgt cgccgctctg catcgcttgg acgaatcgcg ctggctgctc
     1801 tgcggacgcc tgacccaagg gattggcttt gctgcgatct acactccgat gcagtgggag
     1861 gtcgaatatc tcgacgtgcc gcacacacgc gcgttcgtca gcggtgccag tgagccagaa
     1921 cgcggcgtcg cgctgctggt aggcgcggac ggcgtcgcgt tgcgcgtcgg cgcaactgcc
     1981 acgacgagca ccttggcgaa tggctctccc gatctcaccg ccgctgcgat ggacgtgctc
     2041 gcgcgtgagt gggtagcgag cctcggcgcg ctctgggtgc gtgacgccga gcgcgaaatg
     2101 gagtggcagc gagtgtggag cgcgcccgaa tggaagacgc ccttcatcag catgatggct
     2161 gatgccggca tggtcgtcgc catgaccgtc gacggcggca tcgtggaagg acgcgctgcg
     2221 tggcacgcgt tgcgcggccg cgtgagctca gggccgcccc gcgcgcgcga ctccggctca
     2281 tctttcccgc cgcggtgagt gcacccacag tcgcgcacca acgtccggca accgcaatgc
     2341 gcgccgctcg ggtcgttcgc agcgctgcga gttgctcacc tgtgctagga attgacgatg
     2401 cgaacctggc tgctcggtgg gctttcgtgc gcaatcctgt ctgccgcgtg ctcatccgat
     2461 acctcgcatt caggtggtag tggcggcgcc gcaaacggtg gttcgacggc gaacagcggc
     2521 ggcagcgttg ccgcgggagg ctccggcgca ggcggcaaga ctaccggcgc gggcggtgcc
     2581 gtgaactccg gtggcatcac gagtaacagc ggtggtgcga acgccggcac gtcgtctcgc
     2641 ggaggttcgg ccggaggtgg cggcacgagc gccgggggcg gcgctccggc gaacggcggt
     2701 accaatgcgg gcggcaaggc gagcggtggc gcgagcgcag gcggtcgcgc ttcgggcggc
     2761 ggcggtggcg gcgggaaatc gagcggtggc agcgccggag ccgccggtgc gagcaccggt
     2821 acaggtggag gcggcgcgac ggtaggctgc agcgcctcgg cgacgggcta caagctcgtt
     2881 tggaacgacg agttcgaggg acccgcgggc agcgcggtgg acgccgcgaa gtggacgcac
     2941 gacatcggag gaaatggctg gggcaacaac gagctcgagt actacaccga cggcgcagcg
     3001 aacgcggcgc tcgacggcag cggcaacctc gtcatcaccg cgaaaaagca aacgatgagc
     3061 ggcaaggact acacctccgc ccgtctcaag actcagggcc tttactcctt cacgtacggg
     3121 cgcgtcgaat cgaggatgaa gattccgtca gggcagggga tgtggccggc gttctggatg
     3181 ttggggagcg acatcgacaa gaactcgtgg cccgactgcg gcgaaatcga catcatggag
     3241 aacatcggca aagagccgaa catcatccac gcgacgattc acggccctgg ctattcggcg
     3301 ggcgcgggcc ccaccaagca gtactcactg cccgatccgg tcgcttccgc gttccacgtc
     3361 tttgccatcg aatgggaaga aggggccatt cgttggtacg tcgacgacat gctctattcg
     3421 accaaaacga aggcggatat tccgagcagc gcgacgtggg tgtactcaca cccgttcttc
     3481 cttctcctga acgtcgcagt cggcgggggg tggccaggaa accccgactc gaccaccatg
     3541 ttcccgcaaa cgatgacggt cgactacgtg cgagtctgtc agaagtagcc tcgcgcctac
     3601 tctgctcggt tgtttcgctc ccgctcgccg acttggctcg gtcgtctcgc gccgaacgcc
     3661 cgcccacttg gctcgttggt ctcgtcccgg gcgtccgcgc gtcgcggtcg gcggggcgga
     3721 taggaaccaa ccccgactcg accacgacgc tgtttcggcg attgcgtcca caagcgcgtc
     3781 gctcagaaag agtcgcgcaa agggtgcctc cggacgcggc cgctcacggc cccatcaagt
     3841 gacattcggc gcagccgaaa cccttgtgcg tcgcgggatc gtacggctgc tcgccgagca
     3901 gtgacgccat ttcggggacc accttttcca tcatgaattt cgtgatctcg ggggtcttct
     3961 gcatgtgctt tttgaagctg tcggtgtacg agagcttcgg cagcttcggg ttcggcatct
     4021 tgaaggtctg gttcttcgca ccgtcgccgt gacaggtgac gcacttcatg tcgccgtagc
     4081 gcttggcgtc cacgctggcg aagagttcct tcatgcgcgg catcacgacc gtcttcatgt
     4141 gctcgaggcg ctgtgtttta tccatcgcgt cccagttgat ggcaggtttc gcagccgagg
     4201 gtgcggcgct ggcttctgga gtggtccccg gcgcgggcgc ggcggaggcg gccggagctg
     4261 ccgacgcggg gggaggcgcg acgggcgggg ctgccgaagc gctcgcgggc gctgctggca
     4321 cgggcggcgc ggcttccggg gagggttgcg tgccgccaca cgcagaaacg accaggggga
     4381 cacaggcgat tagagcaaga cacgagtcga tgagtttcac tggatctcct cgttcgcgac
     4441 gacgcgcgaa atcggcggcg acgctatcac gcgtcgcttc cgcgttgaag tcacgcgatg
     4501 agcgccgcct cggctgcgtg gggggccgct tcgcagaccg cgcgcgcggc ctcgagatcg
     4561 gggctttgct ggaggcgttg gatcagcgcg tacacctcgg tggtttcacg ttgcagcgcc
     4621 gaccagcgtt cggccgcgtt ggaagtcgaa atggcgcgtt ttgcggcgcg cgcgaagacg
     4681 acctcgtacg ccgcttcatc gaggcgaagc gcgaattgat cgaggagccc gtgcggcatg
     4741 gccgcgcccg ctccctggag cagcaggcgg ttcacgaggt tcatgcacag gctttggttt
     4801 gcgcaacgaa aggggtgcag gtgcacgaag cggaagtgaa aatgggagag ggcgcgcctc
     4861 aacctctcca cgtcctcgtg ctcggccgcg tcacgcgcgt cgacgtaggc cgcgtacagg
     4921 cggtcgagat gcgccggctg gagaggcctc ggcggtaaga accaggcgcg gctctcggtc
     4981 tcgtgcgaag cgcgacctcg aacgatgcgt ccccacgaga gtccgcgctc gtcgatcacc
     5041 tcgcgagcgt cttcgagcgc agcgggatcg tgggcgcaca cgagcgcgtg gtaaatgcgg
     5101 ccgccgacga agggcactcc gacacgcggc cctgcgagca gaatcgtttc tccgtcgcga
     5161 acctcgctcc gcacttcggt gacgtacaga gctccgggct gcacgccgct gctcgtgcga
     5221 cttgcgggac gatagaagtt gtcgagcgac gcgcccatca gcgcgacgac gtgcaaacgc
     5281 tgtagggcgc gaacgaagct ctgcgtgtcg ttcgccccgt cgagcgcgtg cgcgctgagt
     5341 tcgcggacgt cgcgcatgtt ttcccagtaa cggcgatcga tttcgtaagg ggtgccgacg
     5401 aaccgtgtca cctccgttcc cgaaatccgc ccctcgagct cggtgagcga gaacggcgcg
     5461 aacagcattc gagcgaacgc ggcggacacg agccgctcga gctcggtctg cgcgcccgtc
     5521 gcttcacggc gagcgtcggc gagcgtgcgt tccccgttga acagcgccag cagagcctcc
     5581 gcagccggct ctttcacccc atcgacctcg agtgcaccac cgagcgtcct cgacgcgagc
     5641 tcgacacccc ccggcgttgc tcgcacggtc aagtcgcgtg gaggtaggag gcggtccgtg
     5701 tcgcgcaggc cgaagcgcac caccctcgct gcaaagaggt agtcctcgtg tgaggtcgct
     5761 gcgcgctcga ggaaggcctc gtagttgcgc ggaggtcggg tcgccgtaac gacggcgtcg
     5821 atgcgcgccc atacgtcatt cgcgggattc ggcggcgagt tcatggcgga gccacgcaac
     5881 gctagcccga atgggaccca aaatcgcggg ccgctagcgc gccgagggaa gttcataata
     5941 acttcgggcc cgtgactctc tgcggcggct tccccggact tgcgctcgtc gtgctcggcg
     6001 caaatgccgc cgcgcccgtg cccgcgccaa tgccgacccc cgtcgaagtg gagctcgagg
     6061 ctccttccgg gtgcgccgat tcacgaagct tcttcgaacg gttgcgcgcg cgaaccgatc
     6121 gcgtgcgcct tgctcaacct ggcgagcgcg ccctcatcat cgacgtgaga gtgtcgcgtg
     6181 tcggggcgaa ggtccacggt gagctccgct tccgccagga cgcgacggag agcgcaacgc
     6241 ggcaagtcga tggagcgacg tgtgaagagg tggttggcgt attcacgctc accgcagcgc
     6301 tggcgctcga acgactcaac gcagagtcgg agtccgggaa tgccgcgctc gcgatcgacg
     6361 gcgtggatgg acaaggtggc gcgagcagcg ccggttcggc gaaagcgcaa gcaggcgctc
     6421 ccagcgcagg cagcggtggc gcggccgcgg gcgccggcaa cgggaatgga aagaccgttt
     6481 ctgaaggcag cgggtccgac gacgagcctc cagcagagac gtgggagaca gatccgatgg
     6541 gcgggctcct ccgacgtacg atccacgtag agctcggcgc gggagttggg ctgacccagg
     6601 tcttgtcgcc tcttttggcg accggcgtcg gaccgctcgt tcgcgtgcgc catcgtcgca
     6661 tggcggtcgg gctatcttcg ctctattggt cgagtcgtct gaccaacgcc gacacttcca
     6721 cgagccttcg ctccttgggc ttctcgctca gcgcatgtcc gtggtcttgg caggcggagc
     6781 gagtgttctt cgagccgtgc gtgttgggaa acgtaggttt cttgcacgta tctaactcgt
     6841 cagtcgtgaa ctcacgggcg gcggatcgca cactctgggg catcggcgtg ctcggccgtg
     6901 tgcgggcggc gattagcccg gttttggcgc tcgaattgga gggcgggttc cacgttcccc
     6961 tggtggagcg ccgcttcatc acggagcgcc ccgagcgtat cgtcgcggaa accccttgga
     7021 tttctccatt tgtcggcctc gcagccgttg tctcaccttg agtcactagt accgtggccg
     7081 aacgagttca tgatcaaggt ctggccaacc gggaattcag ggagctcatg acgcaccttg
     7141 ctgcgagccg aattgccgac ctcgcggcaa cggcacccgc agcgttgtca gcttcgagtt
     7201 caacttccgg gcaggagcct cgcttggcgt ccgcagaaac caggctgcgc gccatggtgg
     7261 actcctacat cgatttcgtc gctcgcgtgc tgcgcaacgc tggtacgccc gacgccgacg
     7321 tggacgatgc cgtccagcgc accttcatca ccgctgccaa ccgcctcgcg gacattcggg
     7381 tcggcgcaga gaagagcttc ctcttgcaga tcgcgctgca cattgcggct cacgcccgac
     7441 gtaccgtcgc tcgacggcgg gaggtgcatg cggatgaagc gcccgagcaa gtcgaggcct
     7501 tcgcgacccc agaacaactg accgaccaga agcgcgcccg gcagatgctc gaccgcgttc
     7561 tggaccagat ggatgcagat ttacgcacgg tcttcgtcct ttacgaattc gaagagctga
     7621 atatggccga aatcgcaact gctctgcgga tccctcaagg aaccgtggct tcgcggctgc
     7681 ggcgcgcgag ggccgacttt cgccaacgcg tggcacggct agaaggaacc tccgggacga
     7741 ggtgggacga tgagtgaacc gcgacgatta cgtgaggtga gcggcagccc cgtggaacac
     7801 gcgctgctct ccgcgggggc atcgtaccga
//
LOCUS       DASTKS010001091         4815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1288157, whole genome shotgun sequence.
ACCESSION   DASTKS010001091 DASTKS010000000
VERSION     DASTKS010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288157"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..1467
                     /locus_tag="VFQ35_23015"
     CDS             472..1467
                     /locus_tag="VFQ35_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933603.1"
                     /translation="MYRLFGSVVIAALSVVAGCGSDDSSNSDGTGGTQHSGGATAKGG
                     ASTEAGSDNLGGLGAFGGQPGGNDGGAGNGLHEPGDPTAGSSTGATGSGSKAVLNLTV
                     SAPASALALDACAATPAVFADVAPGTYDIELTASTLSKGNVSDEDDNESPSFDDYVIV
                     NLPLPAGDPNEDMRFFMLHGVGDKVSVTLPEAGPVNVYFIDGDNEFNVGTATVSLTGG
                     LAPKQVTVDATANVIAWKSSCRGTAPATVTTSKSPQRVTLMSSTFSSAAGTSDHFVLL
                     RLSNEMQVNDHRFTMLNGIGSSYEFTPYAGNVLRAWLIGASGGGKGSAQLKVEEL"
     gene            complement(1537..2250)
                     /locus_tag="VFQ35_23020"
     CDS             complement(1537..2250)
                     /locus_tag="VFQ35_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933604.1"
                     /translation="MAPPPPRENPATAATIPAQLQVAPTPPREPQSPQSQAREPSATI
                     PAPPPLPSSAPPAPQSKIQQTRPPVPPRRAQMLTESDLVEDDDDAEVGASAHSTAPPQ
                     PAPRGSRLKKILTLVSMTALAAGAWLVHARVPSSNRAAAALSVPAAAVASEQRAAELH
                     SPGDQAAATTGLAAKAGGDPKKAKASSAKKATPKREAKAKTPEKAKTGSKAKATANAK
                     PAKPQQKSASAQPAKKRPT"
     gene            complement(2575..3873)
                     /locus_tag="VFQ35_23025"
     CDS             complement(2575..3873)
                     /locus_tag="VFQ35_23025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9933605.1"
                     /translation="MVAVPRLLVTVLREGGKVGNRTVELEGDRIRVGSHPRNELVISD
                     RLVSRFHCVLGIERGQWTLTDTESLNGTLLLGVPIREAFLPPGECEIAIGDSVVRITQ
                     LSPEKLTRVPPQTSFGEMRGRSLAMRQVFSLLERVAASDTTVLVQGESGTGKELVASE
                     LMRRSPRRAQPFLTVDCSAISANLVESELFGHAKGAFTGADRNRVGAFEAADHGTIFL
                     DEIGEMPLEMQPKLLRVLENREVRRAGENEARKVDVRVVAATNRNLEREVNRGRFRED
                     LYFRLSVFTVRLPPLRERTEDIPDLVRALLERMGATESERLFTPNILAELTRQDWPGN
                     VRELRNYVERAVVLQDATGASRKAAPVEPDRELASDVTIDTPFKTAKEQLVASFELAY
                     LQKLMSWAGGNVSRASRKAKLDRMYLHRLLQRYGIKRDDE"
     gene            4133..>4815
                     /locus_tag="VFQ35_23030"
     CDS             4133..>4815
                     /locus_tag="VFQ35_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933606.1"
                     /translation="MSEPTLTRSGSPECEVLFELFSAPRGRVLLGRLNSGRETGRVVR
                     LRAVDAAALPLASEAVAATRDHDFSCPELLKLLDVAEDGAQSYIASEYIPGISLLELT
                     ARARRQGGMDPAAAVNIALNALRRLDQAAALLAAAGRPRPRLFYADCIWIADYGETLL
                     AEPGVSAYLSGNSAPEAQEVGNEAERHDARAIAVELYQLVSGKLLVGDLASALGNLPA
                     PLARVLTGLL"
BASE COUNT          811 a         1523 c         1648 g          833 t
ORIGIN      
        1 cgttcgcgat atgaacctcc gaaattcggg accgagcccc gcccccagat tccagcactc
       61 caatccgaga cgagaggcgt cgagttgcgc cgcggtggag tcaacgaagc actgcgaagc
      121 tgagcgaccg tggagacgag cgcagctgac gcaggcgttt gagccgggga attccgtaac
      181 gctcccacgc tgcgagccga gccaatttcc cacgagcgag tcggcacatc gcgaaatgag
      241 cgaagcgtgt ggcgcggacg agacactccg caagaaagcc gcagccctca cgtcactgtg
      301 cgttgcacct ttgccacgcg cacagcaacg ccccagtacg aacagagaat ttccgcgccg
      361 caaagcacgt gagtttcgca tgcatcgagt aagcgagagc tatactgaag atcaagccag
      421 cgactctcga accacgcgga gactcgctgg caagaaaggc ggcttaagca gatgtatagg
      481 ctcttcggtt cggtggtgat tgctgcgctg agtgtcgtcg caggttgcgg cagcgacgat
      541 tcgtcgaaca gcgacggcac aggcggaacg cagcacagcg gaggggcgac cgcgaaaggt
      601 ggcgcgtcga ccgaagcagg cagcgacaac ctcggcggct tgggagcttt cggtggccag
      661 ccgggaggaa acgacggcgg agcgggtaac ggcctgcacg agcccggcga cccaaccgcg
      721 ggctcgagca cgggcgcgac tggaagcggc agcaaggccg tgctgaacct gacggtcagc
      781 gcacccgcga gcgcactcgc gctggacgcg tgtgccgcga cgcccgctgt attcgctgac
      841 gtcgctccgg gaacttacga catcgagctg accgcgagca ccctgtcgaa gggcaacgtg
      901 tcggacgagg acgacaacga gagcccatca ttcgacgact acgtgatcgt gaacctgcct
      961 cttcctgctg gggatcccaa cgaggacatg cggttcttca tgctccatgg cgtcggcgac
     1021 aaggtcagcg tgacgctccc cgaggcgggt cccgtcaacg tgtacttcat cgacggcgac
     1081 aacgagttca acgtgggtac ggccacggtc tcgttgacgg gtgggctagc gccgaagcag
     1141 gtcaccgtcg acgcaaccgc caacgtaatt gcttggaaga gctcgtgccg cgggaccgcg
     1201 cccgcgaccg tcacgacttc caaatctccg cagcgcgtca cgttgatgtc gagcaccttc
     1261 tcttccgccg ctgggacgag cgaccatttc gtactgctgc gcttgtcgaa cgagatgcaa
     1321 gtgaacgatc accgcttcac gatgctgaac ggcatcggct cgagctacga gtttacgccg
     1381 tatgccggga acgtgctccg cgcttggctc atcggtgcaa gcggcggtgg caagggctca
     1441 gctcagctca aggtcgagga gctctgatgc tcgactagaa gaggctcgac catggggcgc
     1501 gcgcggagcg agaccttctc gctccacggc gcgagctcag gtcgggcgct tcttcgcggg
     1561 ctgcgccgat gcggactttt gttgcggctt tgcgggcttc gcgttcgccg tagcctttgc
     1621 tttgctgccc gtcttggctt tctcgggcgt cttggccttc gcttcacgtt tcggggtcgc
     1681 cttcttggcc gacgaggcct tcgccttctt tgggtcgccg cctgccttcg cggcgaggcc
     1741 ggtcgtggcg gctgcctggt cacccggtga atgaagctcg gccgcacgct gttctgacgc
     1801 gaccgcagct gcggggacgc tcagcgcagc cgcggctcgg ttgctcgagg gcacacgagc
     1861 gtgcacgagc caagccccag ccgccaatgc cgtcatcgaa accagcgtca ggatcttctt
     1921 caaccgagag ccgcgcggtg cgggctgcgg cggcgcggtg gaatgcgccg aggcaccgac
     1981 ctccgcgtcg tcgtcgtcct cgaccaagtc ggactcggtg agcatttggg cacgccgcgg
     2041 agggaccggc ggacgagtct gttggatctt gctttgggga gcgggcggag cggaagacgg
     2101 tagcggcgga ggcgcaggaa tcgtcgcgct gggctcgcgc gcctgcgact gcggcgattg
     2161 cggctcgcgt ggtggcgtcg gcgcgacttg gagctgagcc ggaatggtcg ccgccgtggc
     2221 cgggttctcg cgcgggggcg gtggggccaa gcggggagca gacgggaccg cggcactccg
     2281 aggcaagggc ggcggccggc gcgacgtcgc tggcggacga gaatcgacgc gtggactcga
     2341 gcgggatggc gcagtcgagg gacgcggagg cgtgctgttg aggcgtgctg cgtcgggacg
     2401 tgaagaaggc tgttggcgcg gatcggcagg gtttgggcgg ctcatgtcca ctgagcaatg
     2461 caggcccgat gccacaccca atctctttta gaatcgtgca cttactttgt tcacacagaa
     2521 gggtgatcac ggcaacaggc cgcaattctg atgcctcgag gcatcaccct gcgctcactc
     2581 gtcgtcgcgc ttgattccgt agcgctggag gagccggtgt aggtacatcc ggtcgagctt
     2641 tgctttccgc gacgcacggc tcacgttgcc acccgcccag ctcatcagct tttgcaggta
     2701 cgcgagctcg aagcttgcga ccagctgctc tttcgcggtt ttgaacggcg tgtcgatggt
     2761 gacgtcgctc gccagctctc gatcgggctc gaccggcgcg gccttccgtg aggcaccggt
     2821 cgcgtcctgc agaacgacgg cgcgctcgac gtagttgcga agctcgcgca cgttgcccgg
     2881 ccaatcctga cgagtcagct cggccaaaat gttcggggta aatagccgct cgctctcggt
     2941 agcgcccatt cgctccagga gagcgcgcac gaggtcgggg atgtcctcgg tgcgttcgcg
     3001 cagcgggggc aggcggaccg tgaacacgga gaggcggaag tacaaatctt cccggaagcg
     3061 cccgcggttc acctcgcgct ccaggttccg attggtggct gcgacgacgc gcacgtcgac
     3121 cttgcgcgcc tcgttctcgc cggcgcggcg cacctcgcga ttttcgagca cgcgcagcaa
     3181 cttgggctgc atctccagcg gcatttcccc gatttcgtcg aggaaaatcg tgccatgatc
     3241 ggccgcctcg aaggcgccga cgcggttccg atccgcaccc gtaaaagcgc ccttggcgtg
     3301 accgaagagc tcgctctcga ccaggttcgc cgaaatggcg ctgcagtcga cggttaggaa
     3361 cggctgcgcg cggcgcggac tgcggcgcat gagctcgctc gcgacgagct ctttgccggt
     3421 tccgctttcg ccctgcacga gcacggtcgt gtcactcgcc gcgacgcgct cgagtaggct
     3481 gaagacctga cgcatggcga gcgagcgccc tcgcatttcg ccgaagctgg tctgcggcgg
     3541 aacgcgcgtc agcttttcgg ggctgagctg ggtgatgcgc acgaccgagt cgccgatcgc
     3601 gatctcgcac tcgccggggg ggaggaacgc ttcgcgaatc ggcacgccga ggagcagcgt
     3661 tccgttcagc gactcggtgt cggtgagggt ccactgccct cgctcgatcc cgagcacgca
     3721 atgaaagcgc gaaacgagtc ggtcggagat cacgagctcg ttgcgcggat gagagccgac
     3781 gcggatgcgg tcaccttcga gctcgacggt gcggttgccc accttgccac cctcccgaag
     3841 aacggtgacg agcagtcggg gaacggcgac catggtcccg agcgaggccg ggttgacccc
     3901 tgtgagcgtc gaggtgtcgt cctcgctttt ttcgcccggt cgcagcggac ggttatccat
     3961 atggctttcc acagtatccc gtgtgccgaa cgctcgtcga gcggctcccc catggttcag
     4021 ggccatagaa ttcacggccc ggcgccttcc ttccggtggg cggccggcgt agtctaggaa
     4081 ggttgcagtg caaaaccgcg actttgggta ggtgacggta cgtcgtgcga ggatgagtga
     4141 gccgacgctg acacgatcgg gttcgcccga gtgcgaagtc cttttcgagc tgttctcggc
     4201 gccacgcggg cgtgtgctgc tcgggcggct caactccggc cgcgagacgg gccgcgtcgt
     4261 tcggctgcgg gcggtcgacg ccgccgcgtt gccgctggcg agcgaggcag ttgccgccac
     4321 gcgcgatcac gacttttcgt gtccggaact cttgaagctc ctcgatgtcg ccgaagacgg
     4381 cgcgcaaagc tacatcgcaa gcgaatacat tccggggatc tcgctactcg agctcaccgc
     4441 gcgggcgcgc cggcaaggcg gaatggaccc cgcggcggcg gtgaacatcg cgctcaacgc
     4501 gttgcgccgg ctcgaccagg cggcggcgct gttggctgcc gccggtcgac cgaggccgcg
     4561 cctgttctat gccgactgca tttggatcgc ggactacggc gagaccctgc tcgcagagcc
     4621 aggtgtctcc gcctacctca gcggtaactc tgcgcccgaa gcgcaggagg taggcaacga
     4681 agcagaacgg cacgacgcgc gcgcgatcgc ggtcgagctc tatcaactcg taagcggcaa
     4741 gctgctggtc ggagacttgg cgagcgctct cgggaacctc ccggccccac tcgcgcgcgt
     4801 gctgacaggc cttct
//
LOCUS       DASTKS010001092         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1512469, whole genome shotgun sequence.
ACCESSION   DASTKS010001092 DASTKS010000000
VERSION     DASTKS010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512469"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1136
                     /locus_tag="VFQ35_23035"
     CDS             246..1136
                     /locus_tag="VFQ35_23035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9933607.1"
                     /translation="MDLDSIRLFVRVAELGSFTRASEHLGLSKARASIRIRELEAELG
                     CRLLLRSTRAVRLTEDGEQFLPRAKRLTAEADELVAMFQAPSTLRGRVRVDLPIRLAL
                     DHVIPRLPEFLAAHPQIELLVSTTDQHVDLVRDGFDCVVRVGKLPDSGLVARRLGTLP
                     MVNCASPTYLRKFGIPRTLADLDRHWLVDYSASLGAAPPTFEYRDGDRYRERPMQSMV
                     TVNGTDAYQAACIAGLGIIQAPRIGTRASLARGELVEILPELECARMPVSVLQAHSRV
                     REPVRALTTWLAAVLAPHLS"
     regulatory      1197..1265
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1418..2698
                     /locus_tag="VFQ35_23040"
     CDS             1418..2698
                     /locus_tag="VFQ35_23040"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9933608.1"
                     /translation="MKRRDRLVALGQWLALGSLVGVLCGAASALFLWLLQLATEFRGA
                     HEGIVYTLPLAGALIGFLYERFGQPIKGGNNLVIDTIHDEGPEIPLRMAPMVLLGTVL
                     THFFGGSAGREGTAVQMGASITDWVSHRLGVSKRVRRQLLAAGVAGGFGSVFGTPIAG
                     AVFGLEFVVLGHVEYDALVPALVAALIGDLTTRALGIGHTAYPAPSPLPLDLILLLKW
                     LVFAVAVAAVTTAFIELLHFLKKRSEALAPRLPVRMLLGGAAVVGLWKLVGTSDYLGL
                     GIPTIVRAFVDPNLAVDAFAWKLLFTAVTLGAGFLGGEVTPLFFVGATLGNLLGRLLG
                     IPIGLSAGVGLAAVFAASANTPLALSIMAMELLGASVFPHVVVVCVIAYLLTGHRSIY
                     PAQRLLRSKAGSGLAAPRALRDLSRNPPPRNPGA"
     gene            2815..3798
                     /locus_tag="VFQ35_23045"
     CDS             2815..3798
                     /locus_tag="VFQ35_23045"
                     /inference="COORDINATES: protein motif:HMM:NF021512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HET9933609.1"
                     /translation="MPTDLQLIAFSGDRRISSGSIEEVLSLLKARFDRAEPDALCFDV
                     QTGAQVDFDLRGTLPEVLARVQPNEQRGRGRPKLGVTGREVSLLPRHWEWLEQQPNGI
                     SATLRRLVEHATKADPGREQARNTRAALSRVLSAMAGNRENYEEACRALFQGDDARFE
                     SLIEAWPSDVRGFALHQAREATRQDRASRANVSAQGVVIRDLYERVWSYGDYAAIGKL
                     VAPRYVVHSDPGDPWEGQTLDHDGYRERVRYSRKAFPDLNFTIEDIVTDGEKVSVRWR
                     ADGTHDGDLKGLPATGKKLSFHGQTVYEFADGRVSGHFQIVDRLGFIEQLR"
BASE COUNT          644 a         1286 c         1218 g          763 t
ORIGIN      
        1 accgcgcgcg cttcgtcgtc tcgcgtatga taggtgatga tgctgtcgac gccgcgctcg
       61 gcgaggtgaa tggccatgct cctaccgagg ccccggctgc cgccggtgat gatcgcaatg
      121 ggatgatgtc gagtcgagtt catcagctgt tttccttccg ctctcccatt cgtagggccc
      181 tccgcgcgac agaaaaagcc cgtggcgctt gacacactgt ttcgcaaaaa gaaacaattc
      241 cgttcatgga cctcgattcg atccggctct tcgtgcgcgt cgcggagctc ggcagcttta
      301 cccgggcgag tgagcacctt gggctgtcca aggcgcgcgc ctcaatccgc attcgcgagc
      361 tcgaggcgga gctcggttgc cgcctcttgc tgcgctccac ccgcgcggtc cgcctgaccg
      421 aggacggcga gcagttcctg ccccgcgcga agcgtttgac cgcggaggca gacgagctcg
      481 tcgccatgtt ccaagcgccg agcactctgc gcggccgggt tcgcgtcgat ctgccgattc
      541 gcttggcgct cgaccacgtc atccctcgcc tccccgagtt cctcgccgct cacccgcaaa
      601 tcgagctact cgtcagcacc acggatcagc acgtcgacct cgtgcgcgat ggcttcgact
      661 gcgtcgtgcg cgtgggcaag ctccccgact ccggcctcgt cgcgcgaagg ctgggaaccc
      721 tcccgatggt caactgcgcg agcccgacct acctgcgaaa attcgggatt ccgagaacgc
      781 tcgccgacct cgatcgacat tggttggtcg attactcggc atcgctcggc gccgcgccgc
      841 cgacgttcga gtaccgcgac ggcgaccgct accgcgaacg accgatgcag agcatggtca
      901 ccgtgaacgg cacggacgcc taccaagccg cgtgcatcgc cgggctcggg atcattcaag
      961 cgccccgcat cggaacccgg gcgagcctag cgaggggcga gctcgtcgag atcttgccgg
     1021 agctcgagtg cgcgcgcatg ccggtgtcgg tccttcaggc gcactcgaga gtccgtgagc
     1081 ccgtacgtgc cctaacgacg tggctcgctg cggtacttgc cccgcacttg agctgagccc
     1141 gccgcggttt cgcttgacag agccgtgtct taggactgaa gctcactcga gcatctttca
     1201 gcgatggcgg tggagtccgc cgataaccgc ggccctttgg gaccgccaat gactcctacg
     1261 acgcagccta tcaggcgccg taggagttca cgtgagccct ccggcgcgaa agccaggaga
     1321 gacgccccgt gaccctgccc cgaaacacat cgagccgccc gagccagcta cgttcattct
     1381 cggccttggc atccccttcg ctcactggag gcacggcgtg aagcgacgcg accgcctcgt
     1441 cgcgctgggg cagtggctcg cgctcggctc gctcgtcggc gtgctgtgtg gcgcagcctc
     1501 ggcgctgttt ctttggctgc tgcagctcgc gaccgagttt cgtggcgcac acgagggcat
     1561 cgtttacacg ttgccgctcg ccggcgctct catcggcttc ctctacgagc gctttggaca
     1621 gccgatcaaa ggcggcaaca atctcgtcat cgataccatc cacgacgagg gcccagagat
     1681 ccccctgcgg atggcgccta tggtcttact cggtacggtg ctcactcatt ttttcggggg
     1741 cagcgccggg cgcgaaggca cggccgtgca aatgggtgcg agcatcaccg actgggtttc
     1801 ccatcggttg ggcgtgagta agcgcgtgcg acggcagctg ctcgcggcgg gagtcgcggg
     1861 cgggttcggt tccgtattcg ggacgcccat cgcaggcgcg gtgttcggcc tcgagttcgt
     1921 cgtgctcgga cacgtcgagt acgacgcgct cgtccccgcg ctcgtcgcag ccttgattgg
     1981 agatttgacg acgcgcgctc tgggtattgg acacaccgct tacccggctc cgtccccgct
     2041 gccgctcgac ctgattttgc tgttgaagtg gctcgtcttc gcggtcgcgg tcgcggccgt
     2101 gaccacggcg ttcatcgagc tcttgcactt cctgaaaaag cgcagcgaag cgctcgctcc
     2161 acggctaccc gtacggatgt tactcggtgg cgcggcggtc gtcgggctat ggaagctcgt
     2221 tggtacgagc gactacctcg ggctcggtat cccgaccatc gtgcgcgcct tcgtcgaccc
     2281 aaacctcgcc gtcgatgcgt tcgcgtggaa gctcctgttc accgccgtga cgctcggcgc
     2341 aggatttctc ggcggtgaag taacgccgct gttcttcgtc ggcgctacgc tcggaaactt
     2401 gctcggccgc ttgctcggta ttcccatcgg gctgtctgcg ggcgttgggc tggctgcggt
     2461 gttcgccgcg tccgcgaata ctcccctcgc actctcgatc atggcgatgg agctgctcgg
     2521 cgcttcggtg ttccctcacg tagtcgtggt ttgcgtcatc gcgtacctac tcacggggca
     2581 ccgcagcatc tatccggcgc agcgattgct ccgcagcaaa gccggctcgg gccttgcggc
     2641 accgagggcg ttacgcgact tgtcccggaa ccctccacca aggaacccgg gggcgtgacg
     2701 ttggactgag aatccggagc tgacgagcgc catcacctat ttttaccagg atattattgc
     2761 gcgccaattt tatcctggta taaaccaatt cactcgaatc aaaggtgaat caaggtgcct
     2821 accgatctcc agctcattgc attttctggc gacaggagaa tttcatccgg atcaatcgaa
     2881 gaggttcttt ctcttcttaa ggcgcgcttc gatcgggctg agccggacgc tctgtgcttc
     2941 gacgttcaga cgggcgccca ggtggacttc gatctgcgcg gcaccctccc cgaggtccta
     3001 gcccgagtgc agccgaacga gcagcgaggt cggggccgcc caaagctcgg tgttaccgga
     3061 cgcgaggtct cgctgttacc tcgacactgg gagtggctcg agcaacagcc gaacggcatc
     3121 tcggcgactc tgcgccgttt ggtcgaacac gccaccaagg cagacccggg acgagagcaa
     3181 gctcgcaaca cgagagctgc gctcagccgc gtcctctccg cgatggcggg caatcgagag
     3241 aattacgaag aggcttgtcg agccttgttc cagggcgacg acgcgcgttt cgaatcgctc
     3301 atcgaagcgt ggccgagcga cgtccgcggg ttcgcgctgc accaagcgcg cgaggccacc
     3361 cggcaggatc gcgcgtcgag agcgaacgtt tcggcgcagg gcgtggtcat ccgagacctt
     3421 tacgagcgag tttggtccta cggagactat gccgcgatcg gcaagctcgt ggctccgcga
     3481 tacgtcgttc attctgatcc cggcgacccc tgggaagggc agacgctcga ccacgacggg
     3541 taccgcgaac gcgttcgcta ttcgcgaaaa gcatttcccg atctcaactt cacgatcgaa
     3601 gacatcgtga cggacggcga gaaggtcagc gttcgttggc gggcggacgg cacgcacgac
     3661 ggagacttga agggcctgcc cgcgactggc aaaaagctct ctttccacgg tcagaccgtc
     3721 tacgagttcg cggacgggcg cgtcagcggc cactttcaaa tcgtcgaccg cctggggttc
     3781 atcgagcagc ttcgctagca ctactccgcc acttttctct ttccattcgg cgctccgtgt
     3841 gctccatagg cgagcgcgat gcgcgacctc agcgagaagt ggcagcgaga cctggaccgg
     3901 tggtttgcac c
//
LOCUS       DASTKS010001093         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1512762, whole genome shotgun sequence.
ACCESSION   DASTKS010001093 DASTKS010000000
VERSION     DASTKS010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512762"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VFQ35_23050"
     CDS             complement(<1..1252)
                     /locus_tag="VFQ35_23050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933610.1"
                     /translation="MIEPHAPAARRCPDCGELSPDEALFCCHCGLSLRSRVEAPEPDP
                     RLAELATLPPLKSLRAPTLMQSLSERPGEHFADPLIGRTVADRYRILEQIGRGGMGVV
                     YRVEHARIGKLMALKLLSGELTRDGVQVARFKREALMASRLSHPNTVQVFDFGNADGL
                     VYLAMEYLRGEDLGRIIRREGALDVDRAARIIIQVASSLAEAHEKGIIHRDLKPENIL
                     IVRGHSGEEVVKVLDFGLAKLRESSELSEVTSRGAIVGTPYFMSPEQIRGEGAEPTSD
                     IYSLGALMYTCLTGDPVFDANTPIGVFTKHLLEQPVPPHVRRPERHIPENLSRLIVTT
                     LEKDPSRRPPSVNALQNALAQSLSTSSSSVQQLIGAGPPQELTEGDAEAATRAEVERY
                     EQKMRRRHRLTQAALVGVGLALLAA"
     gene            complement(1249..3066)
                     /locus_tag="VFQ35_23055"
     CDS             complement(1249..3066)
                     /locus_tag="VFQ35_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933611.1"
                     /translation="MTVCPRCGTENPAQAQACSLCGLAFTNPPLGSAAPPAGARREFA
                     KTLMGQGPMDLSALGVKPVAPPAREPEAARPPASKFNATMMGVAPPPLPPEHAAPALG
                     NSQQPVLESGQAGRANLNATIPFAPPSPDPKLGVTQPLPKPPPAALNPANKTIMGIAR
                     PGIAPLNPGVAKPVAPSAPAPTDSFPAPPPAPLPALPTLSPVNSLEPRDARRTPWLAA
                     VALGGALVLLATAAAAYFLLKGPGPIEARALLDSEGREQLELRCGGCKDGDQIRLNQT
                     VAPFRGGKALLPVPGGLKIGETRFEFALLRSGARKESRVSLSVPLEYRVRADESGLSD
                     AEPHLSVRVEALAGTTVSIDGHPVSLVGGKGSYDIPVTNELSGVDASVRKLDRRVAYR
                     ISPPGSSGESGEVAFQIGITPLSIQAPGESIVIEGSTFMLAGRTAKGATLTVSGRAIP
                     VEPDGRFTQNLSISAVGESNVVVRASAADHAPRLASFRVRRVQSLKDEARSLRPSATQ
                     SYATIVADIERAKGSAVALDGTVSEVGGDDFSTLLLLDTQSGCRAAPCLTRVIHGARL
                     SLKAGDRVSVFGKVRGAVDGPRGAKLPEVFADFVVKGPK"
     gene            3194..>3828
                     /gene="hisF"
                     /locus_tag="VFQ35_23060"
     CDS             3194..>3828
                     /gene="hisF"
                     /locus_tag="VFQ35_23060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET9933612.1"
                     /translation="MLAKRIIPCLDVRNGRVVKGVNFVGLRDAGDPVECAKRYDAEGA
                     DEITFLDITASHEARATLLHVVERTAEVVFAPLTVGGGVRAPEDVKALLEAGADKVSV
                     NSAAVADPSLIERVAGAWGNQVLVVAIDAKRVGDGYEVFTHGGRRGTGIDAVEWAKRA
                     AALGAGEILLTSMDRDGTRAGYDLALLRAIVDAVEVPVIASGGVGTLEHLK"
BASE COUNT          606 a         1228 c         1335 g          659 t
ORIGIN      
        1 ccgccgcgag cagcgcgagc ccgacgccga cgagcgccgc ctgcgtcagg cggtgacggc
       61 gtcgcatctt ttgctcgtag cgttccacct cggcgcgcgt ggcggcttcg gcgtcgccct
      121 cggtgagctc ttgcggcggc ccggcgccga tcaactgttg gacactcgag ctgctcgtgc
      181 tcagcgattg ggcgagcgcg ttttgcagag cattcacgct cggcgggcga cgcgacggat
      241 ctttttcgag cgtcgtgacg atgagccggc tcaggttctc cgggatgtgg cgctcgggtc
      301 ggcgcacgtg aggggggacc ggttgctcga gcagatgctt cgtaaaaacg ccgatcgggg
      361 tgttcgcatc gaacacggga tccccggtca ggcacgtgta catgagcgct ccgagcgaat
      421 agatgtcgct ggtcggctcg gcgccctcgc cgcggatctg ctctggtgac atgaagtacg
      481 gcgtccctac gatcgcgccg cgtgacgtca cctccgagag ctcgctcgac tcgcgcagct
      541 tggcgagccc gaagtcgaga accttgacga cttcctctcc cgaatgcccc cgcacgatga
      601 ggatgttttc gggcttgagg tcgcgatgaa tgatgccctt ctcgtgggct tcggcgagtg
      661 agctcgcgac ctggatgatg atgcgcgcgg cgcggtcgac gtcgagagcg ccttcacggc
      721 ggatgatccg accgagatct tcgccgcgca gatattccat cgcgaggtag acgagcccgt
      781 cggcgtttcc aaaatcgaag acttgcaccg tgttcgggtg cgagaggcgg ctcgccatca
      841 acgcctcgcg cttgaagcga gcgacttgga cgccgtcgcg cgtgagctcg ccactcaaca
      901 gcttgagcgc catcagtttc ccgatgcgcg catgctcgac gcggtaaacg acccccatcc
      961 cgccccgccc gatctgttcg aggatgcgat agcgatcggc cacggtgcga ccgatgagcg
     1021 gatccgcgaa gtgctcgccg ggccgttcgc tgagcgactg catcagcgtc ggtgcgcgca
     1081 agctcttcag cggcggaagc gtcgcgagct cggccagccg cgggtcaggt tctggtgcct
     1141 cgacgcggct ccgcagcgaa aggccacagt gacaacagaa cagcgcttcg tccggtgaga
     1201 gctcgccgca gtccgggcac cttcgggctg ccggcgcgtg cggctcgatc acttgggacc
     1261 cttcactacg aagtcggcaa acacctcggg cagcttcgct ccgcgcgggc cgtcgacggc
     1321 gccccgcact ttgccaaaaa cgctgacgcg atcgcccgct ttgaggctca gccgcgcacc
     1381 gtggatcacg cgggtgaggc acggggccgc gcggcatccg ctctgggtgt ccagcaagag
     1441 aagcgtcgaa aaatcgtcgc cgcccacttc gctgacggtt ccgtcgagag cgaccgcgct
     1501 gccctttgcc cgctcgatgt cggcgacgat ggtagcgtac gactgcgtcg cagaaggtcg
     1561 cagtgagcgc gcttcgtcct tgaggctctg gacgcgtcga acccgaaacg atgccaagcg
     1621 gggggcgtgg tcggcggcgc tcgctcgcac gacgacgttg gactcgccga ccgcggagat
     1681 cgagaggttc tgggtgaagc gcccgtccgg ctcgaccggg atcgctcggc cactgacggt
     1741 cagcgtcgcg cctttcgcgg tgcgccccgc cagcatgaaa gtcgagccct cgatcacgat
     1801 gctctcgccc ggcgcttgaa tcgaaagggg agtgatgccg atttgaaacg cgacctcgcc
     1861 gctctccccg gagctgccgg gcggcgagat gcggtaagcg actcggcggt cgagcttcct
     1921 cacgcttgca tcgacgccgc tgagctcgtt cgtgaccggg atgtcgtacg agccttttcc
     1981 gccgacgagc gagacggggt gaccatcgat gctcacggtc gtgcccgcga gcgcttcgac
     2041 tcgcacgctg aggtggggct cggcgtcgct cagcccggac tcatcggcgc gcactcgata
     2101 ctcgagcggg acgctcaagc tcactcgaga ctctttgcgg gcgccgctgc ggagcaacgc
     2161 gaattcgaag cgtgtctcgc cgattttcag gccgcccggt accggtagca gggcttttcc
     2221 ccctcgaaac ggcgcgaccg tctggttcaa tcggatttgg tcgccgtctt tgcacccacc
     2281 gcaacgcagc tcgagctgct cgcgcccctc cgaatcgagc aaagcgcgcg cctcgatggg
     2341 ccccggcccc ttcaacaaga aatatgcggc cgcagccgtc gcgagcagca ccagcgctcc
     2401 accgagcgcc acagcggcga gccagggcgt gcgccgagcg tcgcgcggct cgagagagtt
     2461 cactggggag agcgtgggca gcgctggtaa cggtgctgga ggcggcgctg gaaaactgtc
     2521 ggtcggcgcg ggcgcggagg gcgctaccgg ctttgcgact cccgggttga gcggggcaat
     2581 cccgggccgc gcgatcccca tgatcgtttt gttcgcgggg ttcagcgccg cggggggcgg
     2641 cttcgggagc ggctgggtta cgccgagctt cgggtccggt gaaggaggtg cgaacggaat
     2701 ggtggcgttg aggttcgctc ggcccgcctg tcccgattcg agcacgggct gctgactatt
     2761 gcccaacgcg ggggcggcat gctccggcgg caggggtggg ggtgcgacgc ccatcatcgt
     2821 ggcgttgaac ttcgacgctg gggggcgggc cgcttcgggt tcgcgcgcag gtggcgcgac
     2881 tggcttcacg ccgagcgcgc tcagatccat cgggccctgt cccatgagcg tcttggcaaa
     2941 ttcccggcgc gcccctgccg gcggagccgc agagcccagc ggcgggttcg taaaagccag
     3001 cccgcagagg ctacaagcct gcgcctgagc cgggttttcg gttccgcagc gagggcaaac
     3061 ggtcatccca caatcactat accgcgtgga ctgcggcgcg ggtgagcgag aacgaagggg
     3121 cgcgccgttc gcgctcgcaa ggactttgtc caatttcagg tgcttcgctt aaggatgtgc
     3181 caagaaaggc cccgtgctcg caaaacgcat cattccttgc ctggatgttc gcaacggtcg
     3241 cgtcgtaaag ggcgtcaatt tcgtcggcct gcgggacgct ggcgacccgg tcgagtgcgc
     3301 caagcgctac gacgccgagg gcgcggacga gatcaccttt ctggacatta cggcttctca
     3361 tgaggcccgg gcgaccttgc tccacgtcgt ggaacgaacg gcggaggtcg tctttgctcc
     3421 cctaaccgtc gggggcggcg tgcgggcgcc ggaggacgtg aaggccctgc tcgaagccgg
     3481 cgcggacaag gtcagcgtca attccgccgc cgttgcagat ccgtcgctca tcgagcgagt
     3541 ggctggcgcc tgggggaatc aagtgctcgt ggtggccatc gacgccaaaa gggtggggga
     3601 cggatacgag gtatttaccc atggcggccg tcgtggaacc gggatcgacg cggtcgaatg
     3661 ggcgaaacgc gcggctgccc tcggcgccgg cgaaatcctt ttgactagca tggatcggga
     3721 cgggactcgc gccggctacg acctcgcgtt gttgcgcgca atcgtcgacg cggtcgaagt
     3781 tccggtcatc gcgtcgggtg gcgtggggac gctcgagcac ctgaaaga
//
LOCUS       DASTKS010001094         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1065013, whole genome shotgun sequence.
ACCESSION   DASTKS010001094 DASTKS010000000
VERSION     DASTKS010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065013"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..856)
                     /locus_tag="VFQ35_23065"
     CDS             complement(275..856)
                     /locus_tag="VFQ35_23065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9933613.1"
                     /translation="MRLIRFWRSGDRKAGDRLLRRYEGLLYRFFSRRVSRDIDELVQR
                     TLLACAQSVGRFEGRSSFKSYILGIAHNQFLASLRARGSTDADPPTLATSPEHTPSQL
                     AAFREEQLILVLALIKLEPEFLSPLKLFYWGGRTVEEIAQELSIPVGTVKSRLARGRV
                     LVKENVMRMNIAPSLRDEALREVVEWTNSRVEG"
     gene            1627..1890
                     /locus_tag="VFQ35_23070"
     CDS             1627..1890
                     /locus_tag="VFQ35_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933614.1"
                     /translation="MVKRDKAGGVLALKGDRDKAMEDAKRQMASNCPNGYEIVGEEMA
                     KVGETTDSAEDTNFGKKGASKTGSTVTQDLKEYRITYSCKSAE"
     gene            complement(1972..>2499)
                     /locus_tag="VFQ35_23075"
     CDS             complement(1972..>2499)
                     /locus_tag="VFQ35_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933615.1"
                     /translation="TPDFLLVDDDERFRERLLKAFRQRGLEAEGARDAEQALALVRLK
                     RFRRALIDLRMPGHDGLSLLRALLEHDPELYVVVLTGFGSIATAVEAMQLGARNYLTK
                     PCDADRILAAFSAEPAAEGQEPVFETPSLARIEREHIERVLRECSGNISKTARVLGIH
                     RRTLQYKLAKFPVNR"
BASE COUNT          498 a          765 c          815 g          421 t
ORIGIN      
        1 ccaagtcgac aagcccgccc ccaaactcac gacctcgaag ggtcgacgcc ggaaagttcg
       61 ctgagccagg gccgcaggcg aactcgggaa ctcgctagct agcggggtgt cccaagttcc
      121 tgcccgtcgc gagccaagaa acgcgagcga gaagcgcgcc cgaggagtga gcaccggagg
      181 aaggctgaag cctttcgagg agcgcagcga cgaggccgtg cttcgcagcc gcaggatctt
      241 ggctcgcagc agggcgggga acttgggaca ccccctagcc ttcgacccga gagttcgtcc
      301 actcgacgac ctctcgcaga gcctcgtcac gaagacttgg cgcaatgttc attcgcatga
      361 cgttctcttt gacgagcacg cgcccgcgcg ccaggcggct cttgaccgtg cccaccggga
      421 tcgagagttc ttgcgcgatc tcttcgaccg tgcgcccacc ccagtaaaat agcttcaacg
      481 gactcaagaa ctctggctcg agtttgatga gcgccaacac caagatcagc tgctcttctc
      541 ggaacgccgc gagctgactg ggagtgtgct ccggggacgt ggcgagcgtc ggcggatcgg
      601 cgtcggtcga cccgcgcgcg cggaggctcg ccaggaactg gttgtgcgcg atccccagaa
      661 tatagctctt gaagctcgaa cggccctcga agcgccccac cgattgtgcg cacgcgagca
      721 gagttcgctg gaccaactcg tcgatgtctc gcgagactcg acgcgagaaa aagcgataca
      781 ggagcccctc gtagcgccga agcaggcggt cgcctgcctt gcgatcaccc gaccgccaga
      841 agcggatgag gcgcatctcc gtagtttctt caatttccat agtcgaagcc cgcgcttgcc
      901 ctgatcgttc ttggaacatc tgggaatgga tcggagcgta acctggatga ctcggcgctt
      961 cgagacgcgc caagcagtca ggtgtgcgcg tacgccgcgt tgtcgcagca tgcgcgacat
     1021 agctccgtgc cgaagcacac cgacgtccat tggcggttgc tcgggagtaa cgaatcgagt
     1081 ccgaaatact gaatgtcgcc gagacacctg ctctttcgcg gcacggagtg cggagtcagc
     1141 gccgctgaaa tcggccagcc cgccggctcc cgcatctcga aagttcgcgc gccgtgctca
     1201 cggggccgcg cagctgtcac ctcgtggggt gacccacgtc gagcaaagcg aggtgcggct
     1261 cgacgatgca ccgaattccg gggcgcatgg ggggagtttc ggcgtgccgc cgcgctcgcg
     1321 cgacggttgg cgatcgcggg agaaaacggc ttcacggaag cggcgacttc tatttgtttc
     1381 gaacacttgg tattggagag cgcagtgaaa cccgggcgtt gacacccggg cctggtggca
     1441 aacagaacca agccggtcgg catcgagagc ccgaagccaa aatcgaagca acgagcgcgc
     1501 gagcagccca cggggaaggt tcgaagggtt aaccggaggc gagtggagat gaataagcca
     1561 gtgattgcgt gcgccgtcgc tctgttcggc gctgtggggt gtttcatgat tcagggtcgc
     1621 gctgaaatgg tgaagcgcga caaggccggc ggcgtgcttg cgctcaaagg cgatcgcgac
     1681 aaggcgatgg aagacgccaa gcgccagatg gcctccaatt gccccaacgg ctacgagatc
     1741 gtcggtgaag agatggcgaa ggtcggcgag accaccgact ctgccgagga cacgaacttc
     1801 ggcaagaagg gcgcatcgaa gaccggttcc accgtgacgc aggacctgaa agaataccgc
     1861 atcacctact cttgcaagtc ggctgagtag cgagcggtag agccgctggt cgcgcgtatc
     1921 cggttcggcg cgcgaccagc gaacctgccc tatttggcgc aagcttcgcc ctcagcgatt
     1981 caccggaaac ttggcgagct tgtattggag cgtgcgccgg tgaatgccga gtacgcgggc
     2041 ggtcttcgat atgttgccgc tgcactcgcg cagcacgcgc tcgatgtgct cgcgttcgat
     2101 ccgagcgagc gaaggtgtct cgaatacggg ttcttggcct tcggcggcgg gctcggcgct
     2161 gaaggcggcg aggatccggt cggcgtcgca gggcttcgtc aaatagttgc gcgcgccgag
     2221 ttgcatcgcc tcgaccgcgg tcgcgatgct tccgaaaccg gtgagcacca cgacgtagag
     2281 ctctggatcg tgctccagga gcgcgcgcag caaagacaac ccatcgtgac ccggcatcct
     2341 caaatcgatg agggctcggc gaaaacgctt gagccgtacg agcgccagcg cctgctcggc
     2401 atcgcgagcg ccttccgcct cgaggccgcg ctgccgaaat gccttcaaca agcgttcgcg
     2461 aaaccgctcg tcgtcgtcta ctagcaagaa gtcgggtgt
//
LOCUS       DASTKS010001095        10216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_618113, whole genome shotgun sequence.
ACCESSION   DASTKS010001095 DASTKS010000000
VERSION     DASTKS010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618113"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFQ35_23080"
     CDS             complement(<1..874)
                     /locus_tag="VFQ35_23080"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9933616.1"
                     /translation="MTPLEAQVKRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGAV
                     KARGEQRRARSDWMKVERERGISVSASAMTFDFEGNTFNLLDTPGHQDFSEDTYRTLT
                     AVDSAVMVLDAARGIEEQTRKLFEVCRLRNVPIITFINKLDREGREPFDLMDEIEQSL
                     AIDVTPASFPIGMGKGFLGCYDLFHDRLILMERARERLGDPGEVCEGLDDPKLDQLLP
                     ESAVARLRDEVTMARGLCPDFDLQSYREGHMTPVFFGSAVNSFGVRELLIGLSELAPP
                     PRAQRTREREISPTE"
     gene            994..3462
                     /locus_tag="VFQ35_23085"
     CDS             994..3462
                     /locus_tag="VFQ35_23085"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9933617.1"
                     /translation="MENRRIFTGAVALFVAVGLGWYLVAGGQRNATPRPPKLPYRANG
                     IIISAVPAGGLTSGTDRAEGAAGDLLVQNGRLSFVVGGDAPGLERQARHGALLDLTLK
                     DFHADELVDLRPILRESGKPLPLVMVQVTLVREGKWPVVRVEQATGDGRVRVATDFEA
                     APNSSKIELVTRIQNEGDRVLRAVELGERTRWPGAGSFSPHVGFPKLTSKAEVSWVAR
                     EGKQLSYALVFPRGPVQASFIFDIVGQVGQETTALVGDVSVGATIAYRRELLVVAGDM
                     SDIAEMVQRSLGVDIGWVVGRVEPPPAWAKVEARFPDGKPALSVRARSDGSFRLPLPV
                     GDYGLDLRAPGGEDDSQVSVFRDRKTEVKFSAKVPGHLKYQVTDTDGAEIAARLTLRG
                     ISPTKDPELYPVEQASGAKNVVYTRSGIGDVELPAGHYHVVVSHGPEFEIATHDIEVD
                     SQNGAAIHSSLARSVDTTGWLGCDFHLHAAPSRDSSVTLPDRVLSLLAEGIEFAVPTD
                     HNHITDYTRAISEHRAAAELGTISGVEITTGTWGHFNAYPYPLHTAPPPYSGVNPIEI
                     FAAVRARAPGAVIQVNHPRMPGVGYFNRIELNAETGSAATEGASFEFDALEVVNGYDL
                     ENPKFVESNIREFFALLNIGRRYTATGNSDSHRLLINWAGFPRTYVRVPDEQPGKVTG
                     AAVARAVHEGHAIVSNGIFLVVAANGSAGPGDTVTGSRVTLQMEARAPSWVDLSRVEV
                     WVNGGLFSKSPTVAKPTPGGRLVWQVELDLRLDSWISVVAHGDEPMTGVFYGRRVLPF
                     AFTNPIFVDADEDGVFRAPEAPQH"
     gene            complement(3507..4454)
                     /locus_tag="VFQ35_23090"
     CDS             complement(3507..4454)
                     /locus_tag="VFQ35_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933618.1"
                     /translation="MRILAALALGYYFVVGRVGGEISSGPAKVPAVPVGMTSAARTEL
                     DRAIGVTLEAPKSQADAPQRRVTHGGRVVFIPNGCSSVQKPYDLVVHFHGAPTAMEPA
                     FDKSGVGGVLAILNLGIGSGAYEDAFQAPSAFQELLGRVTTVVSEMCPGASMKLGRIA
                     LSSWSAGYGAVWHIIDRQSNADRIDAVLLSDGLHVGFEPDKKRERVVNSASMAAFTLF
                     ADRAVNGEKLFAITHSSIVTPYASTTETANYLLQQENVPRLSLNEQGPRPNMIQISRG
                     DRGALHVLGYAGNDKPDHCDQLHAIGDTLFPYLRDRWAR"
     gene            4567..5988
                     /locus_tag="VFQ35_23095"
     CDS             4567..5988
                     /locus_tag="VFQ35_23095"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9933619.1"
                     /translation="MQRYRIKIGGKPADSSQVELVHSPFDGAPVAEVGVADETHAEAA
                     IVSSIAGFETLRHSPSYVRAEILERIASGIRAREREFASLICAESGKPIRYARGEVAR
                     AITTFTLGAAEARTLSGELLPVDQGAGLEGRLCMYRRVPRGPVLGISPFNFPLNLVAH
                     KLAPALAVGCSLLLKPAAQAPLTAHLLSDVVDEAGAPPGAFNVVHCPPSIGERLVLDP
                     RLPVLSFTGSDALGWRLKGLVPRKQAVLELGGNAPCLVDADVELAAILPRLSESAWAS
                     AGQVCIKAQRFFVHERVFEQFVEGFLAETARVAVGDPGDEKTMVGPLIEARHVERVLE
                     WVDEARRAGARVLTGGRAERQIVFPTVVTDTRPGMRVADAEVFGPVTVVERARDMEHA
                     FELANASRFGLQASLYTRDLGTALRAYERLEYGGVLVNDPTTFRVDNYPYGGTKESGF
                     GREGVRFAMEEFSEPKLLSLRAL"
     gene            complement(5995..7563)
                     /locus_tag="VFQ35_23100"
     CDS             complement(5995..7563)
                     /locus_tag="VFQ35_23100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933620.1"
                     /translation="MRAALASGLILAGRYQIVSALGGTEFGAVYTVSDRVSGRECALK
                     LFHPDLRQTPSAFAAYQEVERTVAALPVDAIARASDFGTDGALGQYFVVSELVGFASI
                     AKLVQQHGRLNLAVYCEALGVLATSLDAAAVKGIVHGDLKPENLFFALEHPGWARISD
                     FGTAALRAALSPRASAAPLGWVSPERMQGSPPSRADDVFALGLVSFYALTGRHYCRSM
                     AEPTVDPALVLRELASERPSAAAHARSLGVELSDALDPWFARALAHDPSARFASASDA
                     AKALSELQRRDGSGLTPGIAAAVAAPLLFQELPQRLTPEKPTTSRAFVPSEPRESGEL
                     PRSSRPPGVAAPRPKPREHVDGLPNAPPVALIAGAASLVLVLAAFGLYGTWRLFARTK
                     PAPAALAAASASAAQVVSAPPPAPAPSAEAVIARPAAHFACTPDECEWIVCDGENVKK
                     GVTTLELPVGKHRCSASRYGFRAAVVEFTLEEGKTTNVVFELLPTKAKHARPKPKAPK
                     GAAAKSAVPGKPKP"
     gene            complement(7563..8714)
                     /locus_tag="VFQ35_23105"
     CDS             complement(7563..8714)
                     /locus_tag="VFQ35_23105"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9933621.1"
                     /translation="MAFRRIALVLSLLLLPAVARAESDSPRTHIVYDGQRLGSIAKRY
                     GVSVDALCNANEIRPTDTLKPGQRLLIPNRGDRDGTRARALLPREGTRRRSSTERPAT
                     HRVEAGQRLDSIARRYNISVDALRQANQLSAKSIIRPGQILRIPDPSGAESSDASDGR
                     SYLRPSSRPGHIELVGYSERFRGQAVDRKGRVVPSAFEGACRVLAATGDRPRLDARLV
                     RLLAEVSDNFGGRPLRIVSGYRTTSFFRDSRHKLSKAVDFSIPGVPNQVVRDYLRTLK
                     NVGVGYYPNSSFVHLDVRDYSAYWVDYAGPGEAPRGKHNQPIDPEEHEHGDDGENASE
                     SGAGALSAHGALPSAESSIGATSLKAAIEAYSLKPEGARTRDAADDTNP"
     gene            9058..10011
                     /locus_tag="VFQ35_23110"
     CDS             9058..10011
                     /locus_tag="VFQ35_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933622.1"
                     /translation="MARKLFEDAVRFEREQRWELAASKLNDAIAVKDTPGLRFHLAHC
                     EDKLGHLVEAMIHYDHARELLASGVKAPDVEEVLEPARKNLELRLPTLLLVVPAEVPD
                     VQVELDGRVLARSVIGRAAPVNPGIHKLLVRAPGHVEYAEEVSILEGERRRISVDLPR
                     APSTSSAQTETPVKGGSVSSRAESSRGVSARTVVLVGETAVTLAALGVGVGFLIAKSG
                     AADRVRRAQAVVDDATPAGGGATACLNPPSMAVKQACSELDDAAGRYDTARHISTFGL
                     VGASVGAAATLLTLVLWPSSPVTAEVNASSGSAFLGVSGRF"
BASE COUNT         1722 a         3267 c         3460 g         1767 t
ORIGIN      
        1 cttcggtcgg cgagatttcg cgctcgcgcg tgcgctgggc gcgaggcggc ggcgcgagct
       61 cggacaagcc gatcaagagc tcccgaaccc cgaagctgtt gaccgcgctg ccgaagaaga
      121 ccggcgtcat gtgaccctcg cggtacgact ggagatcgaa gtccgggcag agcccgcgcg
      181 ccatcgtgac ttcgtcgcgc aagcgcgcga ccgccgactc cggcaacagc tgatcgagct
      241 tcgggtcgtc gagcccttcg cacacctcgc cgggatcccc gaggcgctcg cgagcgcgct
      301 ccatcaaaat caagcgatcg tggaagagat cgtaacaacc caaaaagcct ttccccatac
      361 caatggggaa gctcgcgggc gtcacgtcaa tcgcgagcga ctgctcgatt tcgtccatca
      421 agtcgaacgg ctcgcgcccc tctcggtcga gcttgttgat gaacgtgatg atcggcacgt
      481 tgcgcagccg acacacttcg aaaagtttgc gcgtttgctc ttcgatgccg cgcgcggcgt
      541 cgagcaccat caccgccgag tccaccgcgg tcagggtgcg atacgtatcc tcgctgaagt
      601 cctggtggcc aggcgtgtcg agtaggttga aggtgttgcc ctcgaagtcg aacgtcatcg
      661 ccgacgccga caccgagatc ccgcgctcgc gctcgacctt catccagtcc gaacgtgcgc
      721 ggcgttgctc accgcgtgcc ttcacggcgc ctgccagctg aatggcgccg ccgaagagaa
      781 gcagcttctc ggtgagcgtc gtcttaccgg cgtcaggatg agagatgatt gcgaaggtgc
      841 ggcggcgctt gacttgagct tcgaggggag tcatcggtga ggcgagcggc cgttcgggcc
      901 ggtctccggg ggtcgcgttc tagcttgaga gacgggtgct cgcacgccct cgcgaattgc
      961 ggggcggcga aaaatccgct gtagtgagga ggcgtggaga atcgccgcat tttcaccggg
     1021 gccgtagccc tgttcgttgc ggtcgggctt ggctggtacc tcgtcgcggg agggcaacga
     1081 aatgccaccc cgcggcctcc gaaactaccg tatcgcgcga acggcatcat catctccgcg
     1141 gtaccggcgg gcggcctcac gtcgggcacc gatcgcgccg agggcgcggc aggcgacctc
     1201 ttggtgcaga acgggcggct gtcgttcgtc gtaggcggtg acgcgccggg gctcgagcgg
     1261 caggcgcgcc atggcgccct gttggatctc acgctcaaag acttccacgc cgacgagctc
     1321 gtggacctcc ggcccatcct gcgagagagc ggaaagccgc tgccgctcgt gatggtccag
     1381 gtgacgctcg tgcgagaagg caaatggcca gtcgtgcggg tggagcaggc gactggtgac
     1441 ggtcgagtgc gcgtggctac cgatttcgag gccgctccga acagctcgaa gatcgagctc
     1501 gtcacccgca tccaaaatga gggcgatcgg gtgctgcgcg cggtcgagct cggcgagcga
     1561 acgcgctggc cgggcgcggg gtccttctct ccgcacgtcg gctttccgaa actcacgtcg
     1621 aaggcggagg tctcatgggt cgcgcgagag gggaagcagt tgtcgtatgc tctcgtgttc
     1681 ccgcgcggcc ccgtccaggc gtcgttcatt ttcgacatcg tcggtcaggt ggggcaagag
     1741 acgacggcgc tcgtcggaga cgtttcggtt ggcgcgacga tcgcttaccg ccgcgagctg
     1801 ctcgtcgtcg cgggtgacat gtccgacatc gcggagatgg ttcagcgctc gctcggcgtc
     1861 gacatcggtt gggtcgtggg ccgcgtcgaa ccgccgcccg cgtgggcgaa agtcgaggcg
     1921 cgctttcccg acgggaagcc ggcgctcagc gtgcgcgcgc gcagcgacgg ctcttttcgg
     1981 ttaccccttc cggttggtga ttacggcctc gatctgcgcg cgcccggcgg tgaagacgac
     2041 tcgcaagtga gcgtcttccg cgatcggaag accgaggtga agttcagcgc gaaggtaccg
     2101 ggccacctca agtaccaggt caccgacacc gacggcgccg agatcgcggc ccgcctcacg
     2161 ctgcgcggaa tctcccccac caaagatccg gagctttatc ccgtcgagca agcgagcggc
     2221 gcgaagaacg tcgtctatac gcgctcgggg atcggtgatg tcgagcttcc ggcggggcac
     2281 taccacgtcg tggtcagtca cggccccgag ttcgaaatag ctactcacga catcgaggtg
     2341 gactcacaga acggcgccgc gatccactct tcgctcgcgc gcagcgtcga cacgaccggc
     2401 tggctcggct gcgattttca cttgcacgcc gcgccttcgc gcgattcgag cgtgacgctg
     2461 ccagaccgcg tgctctcgtt gctcgccgaa ggcatcgagt tcgcggtgcc gaccgatcac
     2521 aatcacatca ccgactatac ccgcgcgatc tcggagcacc gggccgccgc ggaactcggc
     2581 acgatctcgg gcgtcgaaat cacgactggt acctggggcc acttcaacgc ctacccgtat
     2641 ccgctgcaca ccgcgccgcc gccgtacagc ggcgtgaacc cgatagagat ctttgccgcc
     2701 gttcgcgcgc gcgcgccggg ggccgtgatc caagtgaacc atcctcgcat gccgggcgtc
     2761 ggctacttca atcgcatcga gctgaacgca gagacgggca gcgccgctac cgaaggcgcc
     2821 tcgttcgagt tcgacgcgct cgaggtcgtc aatggctacg acctcgagaa cccgaagttc
     2881 gtcgagagca acatccgcga gttcttcgcg ctcctgaaca ttggtcgccg ctacacggcg
     2941 accggcaact cggactcgca ccgcttgctc atcaactggg ctggcttccc gcggacgtac
     3001 gtccgagtgc cggacgagca acccgggaag gttaccggcg ccgcggtcgc tcgcgccgtg
     3061 cacgaaggcc acgccatcgt gtcgaacggc atcttcctcg tggtcgctgc gaacggtagc
     3121 gcgggccctg gcgacaccgt gaccggctcg cgcgtgaccc ttcagatgga ggctcgcgcg
     3181 ccgagctggg tcgacctttc gcgcgtcgaa gtctgggtga acggcggcct cttttcgaag
     3241 tcaccgaccg ttgccaagcc cacgcccggg ggacgcctgg tttggcaagt cgagctcgac
     3301 ctcaggctcg acagctggat ttcggtcgtc gcgcacggcg acgagcccat gacgggggtc
     3361 ttctacggcc ggcgcgtttt accgttcgcg ttcacgaacc caatcttcgt ggacgcagac
     3421 gaggacggcg tgttcagagc gccggaggcg ccgcagcact gagcctcgcc gcgggtggaa
     3481 atggctccga agagctcggc cgtgcttcag cgagcccagc gatcgcgcag gtacgggaac
     3541 aacgtgtcgc cgatggcgtg aagttgatcg cagtggtcgg gcttgtcgtt gccggcgtag
     3601 ccgagcacgt gcagcgcgcc gcgatcaccg cgcgagattt gaatcatgtt cgggcgtggg
     3661 ccctgctcgt tcagcgagag gcgcggcacg ttctcttgct gcaagaggta gttggcggtc
     3721 tcggtcgtgc tcgcgtacgg cgtcacgatc gacgaatgcg tgatcgcgaa cagcttttct
     3781 ccgttgacgg cgcgatccgc gaagagcgtg aacgcggcca tggatgccga gttgaccacg
     3841 cgctcgcgct tcttgtccgg ctcgaagccg acgtgcagac catcggaaag gagcaccgcg
     3901 tcgatgcgat ccgcgttcga ctggcgatcg atgatgtgcc aaacggcgcc gtagccagcg
     3961 ctccacgagc tcagcgcgat gcgacccagc ttcatcgatg cgcccggaca catctccgag
     4021 acgacggtcg ttacgcgacc gagcagctct tggaaggcac tcggcgcttg gaaagcgtct
     4081 tcgtacgcgc ccgaaccgat gccaagattc aagatggcga gtacgccgcc gacgccgctc
     4141 ttgtcgaaag cgggctccat ggcggttggc gccccatgga agtgcaccac gaggtcgtac
     4201 ggcttttgca ccgaagagca gccattcgga atgaacacga cacgcccgcc gtgagtcacg
     4261 cgacgctgcg gagcatcggc ttggctcttt ggcgcttcca gcgtcacacc gatggctcga
     4321 tcgagctcgg tgcgggccgc ggaggtcatg cctaccggaa cagcaggcac ctttgcgggc
     4381 ccgctgctga tctctcctcc cacgcgcccg accacgaagt aatagccgag tgcgagagct
     4441 gccaggatac gcatctaggc ttagttatag aagacggtcc aagatgccgt ccaccgaaag
     4501 tcaccgcgtt tctgggattg gtcatacctc ccaaccaaat gcgccacagc cagtcattct
     4561 gtaatcatgc agcgatatcg aattaaaatc ggcggaaagc ccgcagattc cagtcaggtg
     4621 gagctcgtac acagtccctt cgacggcgct cccgtggccg aagtgggcgt tgctgacgaa
     4681 actcacgcag aagccgcgat tgtgagttcg attgcggggt tcgaaacatt gcgacactcg
     4741 ccgagctatg tgcgggccga aattctcgag cgtattgcgt ccgggattcg cgcacgagag
     4801 cgtgagttcg cctctctcat ctgcgctgaa agcggcaagc cgattcgcta cgctcggggc
     4861 gaagtagcgc gcgcaatcac gaccttcacg ttgggcgccg ccgaagcgcg aacgctgagc
     4921 ggcgagcttc taccggtcga tcaaggcgcc ggcctcgaag gacgcctctg catgtatcgg
     4981 cgtgtaccgc gggggccggt gctcgggatc tcgccgttca atttcccgct gaacctggtc
     5041 gcgcacaagc tcgcaccggc gctcgccgtt ggatgctcgc tcttgttgaa gccggcggcg
     5101 caagcgcctc tcacggcgca cctcttgtcg gacgtggtgg acgaggccgg cgcgccgccg
     5161 ggcgcgttca acgtcgttca ctgcccgcct tcaatcgggg agcgcttggt tctcgacccg
     5221 cggttgccgg tgctgagctt taccggaagc gacgcgctcg gctggcgctt gaaggggctc
     5281 gtcccccgca agcaagccgt tctcgagctc ggcggaaacg cgccgtgcct cgtcgacgcc
     5341 gacgtcgagc tcgctgcgat cctgccgcgg ctctccgaat cggcgtgggc gagcgcgggt
     5401 caggtttgca tcaaggcgca gcggttcttc gtgcacgagc gcgtcttcga acagttcgtg
     5461 gaaggctttt tggcggagac ggcgcgagtc gcggtcgggg atcccggaga cgaaaagacg
     5521 atggtggggc cgctcatcga agcgcgtcac gtggagcgcg tgctcgagtg ggtcgacgaa
     5581 gctcgccgcg ccggagcgcg cgttctcacg gggggccgcg ccgagcgtca aatcgtgttt
     5641 ccgacggtcg tcacggacac gcggcctggg atgcgtgtcg ccgacgcgga ggttttcggt
     5701 ccggtgacgg tcgtcgaacg cgcgcgcgac atggagcatg cgttcgagct cgccaacgcg
     5761 agccgcttcg ggctgcaagc gtcgctctat actcgcgatc tcggcacggc gctgcgcgct
     5821 tacgagcggc tcgagtatgg cggcgtgctc gtcaacgatc cgaccacgtt ccgcgtcgac
     5881 aattatccct atggtgggac gaaggagtcc ggcttcgggc gtgagggagt gcggttcgcc
     5941 atggaggaat tcagcgagcc gaaactcctg tctctgcggg cgctctgaac gcgctcaggg
     6001 cttgggtttg cccggaacgg cagactttgc ggccgcgccc ttgggcgcct ttggcttcgg
     6061 acgcgcgtgc ttcgctttgg tgggcaagag ctcgaacacg acgttcgtgg tcttgccctc
     6121 ttcgagcgtg aactcgacta cggcagcgcg aaacccgtag cgtgacgcgc tgcaacggtg
     6181 cttgccaacc ggtaactcga gcgtcgtcac gcctttcttg acgttctcgc catcgcacac
     6241 gatccactcg cattcgtcgg gggtgcaggc gaagtgggcc gccgggcgcg caatcaccgc
     6301 ttctgcgctg ggcgccggcg ctggtggcgg cgccgagacg acctgagcgg cgctagcgct
     6361 cgctgcggcc aacgccgcgg gtgcgggctt cgtgcgggcg aacaagcgcc aagtcccata
     6421 gagcccgaac gcggccagaa ccaaaacgag cgaagcggcg cctgcgatga gcgcgacggg
     6481 tggcgcgttg gggagcccgt ccacgtgctc acgcggcttc ggccgcggcg cggcgacgcc
     6541 gggcggtcgc gacgagcgcg gcagctcgcc cgactcgcgc ggctcactcg gaacgaaggc
     6601 gcgcgacgtc gtcggcttct ccggggtcag gcgttggggg agctcttgaa agaggagcgg
     6661 cgcggcaact gcagccgcga tgccgggagt caggccggag ccgtcccggc gctggagttc
     6721 ggagagcgct ttggcagcgt cgctcgcaga agcaaagcgc gcgctggggt cgtgggctag
     6781 cgcgcgggca aaccaggggt ccagcgcgtc cgaaagttcg acgccgaggc tccgcgcgtg
     6841 cgcggcggcg ctcgggcgtt cgctcgcgag ctcccggagt acgagcgccg ggtcgaccgt
     6901 aggctccgcc atgcttcggc aatagtgtct gcccgtgagc gcgtaaaacg agactagtcc
     6961 gagggcgaag acgtcatccg cgcgcgaagg cggagaaccc tgcatgcgct cgggggatac
     7021 ccagccgagg ggtgcggcgg acgctcgcgg gctgagtgca gcgcgcaacg cggccgttcc
     7081 gaagtcggag atgcgtgccc aacccgggtg ctcgagcgcg aagaataggt tctcgggctt
     7141 caggtcgccg tggacgatgc ccttcaccgc ggccgcgtcg agcgaagtcg cgagcacgcc
     7201 gagcgcttcg caatagacgg cgaggttcaa gcgaccgtgc tgctgcacga gcttcgcgat
     7261 ggaggcgaac ccgacgagct cgctcaccac gaagtattga ccgagggcgc cgtcggtccc
     7321 gaaatccgaa gcgcgcgcaa tcgcgtcgac cggcagcgcg gccacggtac gctccacctc
     7381 ctgataggcg gcgaacgcgc tgggagtctg tcgcaagtcg gggtggaaca acttgagtgc
     7441 gcactcacga ccgctcacgc gatcgctcac cgtgtacacc gcgccgaact cggtgccacc
     7501 gagggcggat acgatttgat agcgtcccgc gaggatcagc cctgacgcga gcgccgctcg
     7561 catcaagggt tggtgtcgtc cgcggcgtcg cgcgtgcgcg cgccttcggg cttgagagag
     7621 tatgcctcga tggcggcctt gagagacgtc gcgccgatcg aggattcggc actcggcagg
     7681 gcgccgtgcg cgctcagcgc acccgcgccg ctctcggaag cgttctcgcc gtcgtccccg
     7741 tgctcgtgct cttcggggtc gattggctgg ttgtgcttgc cgcgcggagc ctctccgggg
     7801 ccggcgtagt cgacccagta agcggagtag tcgcgcacgt cgaggtgaac gaagctcgag
     7861 ttcggatagt agccgacccc aacgttcttc agggtgcgca ggtagtcacg cactacttgg
     7921 ttcggcacgc cagggatact gaagtcgacc gctttgctga gcttgtgcct ggaatcgcgg
     7981 aaaaaagagg tggtgcgata gccgctcacg atgcgaagcg gacgccctcc gaagttgtcg
     8041 ctaacctctg cgagcaggcg tacgagccga gcatcgagac gcgggcgatc accagtggcg
     8101 gcgagcactc gacaagcgcc ctcgaatgcg ctcgggacga ctcggccttt ccgatcgacg
     8161 gcctggccgc gaaaccgttc gctgtagccg acgagctcga tgtgccccgg cctcgaagaa
     8221 ggacgcaggt agctacgccc atcggaagcg tcggagcttt ccgccccact ggggtccggt
     8281 attctcagaa tctgtccagg cctgatgatg ctctttgcac tcagctgatt ggcctgccgc
     8341 agagcgtcga cgctgatgtt gtaacgtcgc gcgatcgagt ccaggcgctg tccggcttcg
     8401 acccggtgag tcgctgggcg ttcggtcgag ctgcgccggc gcgtgccttc gcgagggagg
     8461 agcgcacgcg cgcgtgtgcc gtcccgatct cctcggttcg gtatgagcag tcgttgccca
     8521 ggcttcagcg tgtcggtcgg acggatctcg ttcgcgttgc agagcgcatc gacgctgacg
     8581 ccataacgct tcgcgatact gccgaggcgc tgaccgtcat agacgatgtg cgtccgaggc
     8641 gagtccgact cggctcgagc gactgccggc aagaggagga ggctcaggac caacgcgatt
     8701 cttcggaacg ccacgcggcc gggagcatga cgaaaatgac gccgcgagtc catactctcg
     8761 tcgctcgatt ggggttggta gcccccgagc gctggcgaat gccgtcgttc acggctgttg
     8821 ggtgacactt tgcacgcaac cagatacgga acggcgccga ctttcttgac gcagctcgac
     8881 gccggatcgg agcatcgcgc cgaaagtgcg actgtccgca taggctttcg ttcaggaaac
     8941 tcaatgagtc ttggaattgc ccgaaagagc aggttcgtca ctggcttcgc cgtggcgctt
     9001 gccttcggta ctacgtcgtt cgtcgctcgt gcagagccaa ccgccacgga gctcgccgtg
     9061 gcgcggaaac tattcgagga tgcagtgcgc ttcgagcgcg agcagcgctg ggaactcgcg
     9121 gcatcgaagt tgaacgacgc gatcgccgta aaggacacgc caggattgcg ttttcacctc
     9181 gcgcactgtg aagacaagct cggccacctc gtcgaagcga tgattcatta cgatcacgcg
     9241 cgcgagctcc tcgcgtcggg ggtcaaggca ccggacgtgg aagaggtgct ggagcctgcc
     9301 cggaagaacc tcgagcttcg cttgcctacc ctgcttctgg tggtgccggc cgaggtgccc
     9361 gacgtgcaag tcgagctcga cggtcgcgtg ctagcacgat ccgtgattgg gcgagccgcg
     9421 ccggtgaacc ccggaattca caagctcctg gttcgcgctc ctgggcacgt cgaatacgca
     9481 gaagaggtat ccatcctcga gggtgagaga aggcgaatta gtgtggatct gccgcgcgcc
     9541 ccttcgacgt catcggccca aaccgagact ccggtgaaag ggggctcggt gagctctcgg
     9601 gcggagtcgt cccgcggcgt ctcggcgcgg accgtggtgc tcgtcggcga aacggcggtg
     9661 acgctagcgg cgctgggcgt cggcgtaggc ttcttgatcg ccaagagcgg agctgcggac
     9721 agggtgcgac gcgcccaagc cgtcgtcgac gacgcaactc ccgcgggagg tggagcgacg
     9781 gcatgcctca acccgccttc gatggcggtc aaacaggcct gctccgagct cgacgacgct
     9841 gctggtcgtt acgatacggc tcgtcacatc agcacgttcg gtctcgtggg cgcgtcagtt
     9901 ggcgccgccg cgacccttct cactctcgtg ctctggccga gctcgcccgt cacggccgag
     9961 gtgaacgcgt cgagcggcag cgcgttcttg ggtgtgagcg gccgcttctg acaggcgctc
    10021 gccccaaaac ggcgtgacca agcgccggaa cacagctgag ggcgcgccct aaccacgcgc
    10081 ccgcctcgca agcggtctcg gctcggtcgc gactgacttg actgcgccgt ccgtcacaat
    10141 ctgaaccagt acgatgcggg tcttgccacg cagctacacc caagctcgct cggtggacgg
    10201 aaccaagtca gacacc
//
LOCUS       DASTKS010001096         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_394152, whole genome shotgun sequence.
ACCESSION   DASTKS010001096 DASTKS010000000
VERSION     DASTKS010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394152"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            785..2641
                     /locus_tag="VFQ35_23115"
     CDS             785..2641
                     /locus_tag="VFQ35_23115"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9933623.1"
                     /translation="MKHWSAGAFATQGPRWAVLTALLSFGCASNITDGSGSTPGAGSG
                     SNPSGGSANGASGSTSNNGAGSNAGGNSNNPSSGGSTSSNGNGGSGNVAGADGPPPPV
                     QGQPSSQVCPTAQVPTTALRRLTRFEYANSVKLLLNVDTSSVSDLPADEVTEGFDNNA
                     ALLSVSSLHAEKYTLVSETLAKLAVANASLVACDTSKKTEDACASDFAKSFGRRAFRR
                     PTTSDDEALLLAAYKAGKDGGSHAEGIEVMIRAALQSPDFLYRLEITTPTNTAAKLVP
                     LSQFELATRLSFMIWGSGPDDALLDAAARGELGSKEQVATKAKAMLSDPKARAAISNF
                     YNQWAGTSRLAITTKNTTLFPNYSNDVKDAMMKELPSFIEYVLWNSDHKFSTLLTSQV
                     GFVTPALAKVYGVPAPTGTTPQMVTFPSEQGRAGLLTQAGVMSVQAHPDQTSPVLRGK
                     FVRTKLLCQPPPPPPPDVEIKVPDASPGSTARDRFGAHLQAGTTCNGCHTLMDPIGYA
                     FENFDALGEFRTTEANKPIDASGEVIASADPALGGKFTGVRELANKLVQSDLVRDCVA
                     TQWFRFAAGRFEAEGDACSLSTLQERFASSGGDLTELVVGLTQTDSFWYRSP"
     gene            2661..>3010
                     /locus_tag="VFQ35_23120"
     CDS             2661..>3010
                     /locus_tag="VFQ35_23120"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9933624.1"
                     /translation="MNPMRFTRRNLLRGGLAATAAIPLLNAQRVEGQSAQPIRRFVVF
                     TTPNGTRNSLFWPTGSETSYTLPAITKDLEPLKSKLLFLKGIKLNDALQNGALGGTVG
                     SEHARGTGGMLTARP"
BASE COUNT          565 a          979 c          949 g          517 t
ORIGIN      
        1 ctcgagccct ggccacgaga atgcgcacga gaccgaaacg ctgcgcggct cgttcttcac
       61 gcaccatctt cattcacttt tggagcgcag ggttgctcgg cgcggccgag cacggcggcg
      121 atggactcgc taacgctcgg cgaagcgttc gagtacgcgc gagcgttgac agagcgcggc
      181 gccagtcgag gtcgttggag gaaacgactt ggacactcgc atcgacgacg cgggcgtgac
      241 ttgagtgtca cacgagttcg aagccagcgc tgacgagttc acggtcttcg gcttcgtcca
      301 ctcacccgag ggatatacaa accgagggat atacggttgc cgtgactcgc tcgagatcga
      361 tcccgagctg actgagtacc cggcgtcgtt cgagagcact cgcgttcgcc gtcgctacgt
      421 ttgcggcgcg acttgcaagc tcgacgtgct ctcgagtcct ttcggcgacg agagtccagg
      481 cgcaccaaaa cttcgtacgc gcggcaccgt tccgcgatgg ccgagtgcgg cgcgagcgcg
      541 cctggcgaag ttggcgagtg aggcgttgcg gctttgcctg gcgttggatt tatccgctca
      601 cactcgtcca atcgactggt gtgccacgaa tattcaggaa tgggcgacta actttcggag
      661 gacgcaaatt ctttgagccg cgacccgacg gggacgcgtc cgctccctgc tagtaggagt
      721 ctgccaatct gaactggagg ctccgggcaa agagctgcca tcgtcggaac aggaatcgct
      781 cgtcatgaag cattggtcag ctggagcatt cgcaacacag ggtccgcgct gggccgttct
      841 gaccgccctg ctcagctttg ggtgcgcgtc caacataacg gacggctcgg gttcgactcc
      901 gggcgccggg tcgggctcga atcctagcgg cggttcggca aacggcgcga gcggaagcac
      961 ttcgaacaac ggtgcaggga gcaatgctgg aggcaactcc aacaacccct cgagcggcgg
     1021 gtcgacgagc tcgaacggca acggcggctc gggaaatgtc gccggcgcag atggccctcc
     1081 tccgccggtt caagggcagc cgagctcgca agtgtgtcct accgctcagg tgccaaccac
     1141 cgcgctgcgg cgtctgacac gcttcgagta cgcgaacagc gtcaagctgc tgctcaacgt
     1201 cgacacgagc tcggtgagcg acttgcccgc cgacgaagtg accgagggct tcgacaacaa
     1261 cgcggcgttg ctctccgtat cttcgctgca tgccgagaag tacacgctcg tgtcggagac
     1321 cctcgcgaag ctcgccgtcg cgaacgcatc gctcgttgcc tgcgacactt cgaaaaagac
     1381 cgaagatgcg tgcgcgagcg atttcgcaaa gagcttcggt cgtcgcgcct tccgccgtcc
     1441 gacgacctcc gatgacgagg ccttactgct ggcggcgtac aaggccggaa aggacggcgg
     1501 cagccacgcc gaaggcatcg aggtgatgat ccgtgcggcg ctgcagtcgc cggacttcct
     1561 ctaccgcttg gaaatcacga ctccgaccaa cacggcggcg aagctcgtgc cgttgagcca
     1621 gttcgagctc gcgacgcgct tgtcgttcat gatttggggt tcggggcccg acgacgcgct
     1681 gctcgacgcc gctgcacgcg gtgagctcgg ctccaaagag caggtggcga ccaaggccaa
     1741 ggcgatgctc tccgatccca aagcgcgcgc cgcgatctcg aacttttaca atcagtgggc
     1801 cggcacgagc cgtctcgcca tcacgaccaa gaacacgacg ctgttcccga actattcgaa
     1861 cgacgtcaag gacgcgatga tgaaggagct tccgagcttc atcgagtacg tcctttggaa
     1921 ctcggaccac aagttcagca cgctcttgac ctcgcaggtc ggcttcgtga ccccagcgct
     1981 cgcgaaggtc tacggcgtcc ctgccccaac cggcaccacg ccgcagatgg tgacgttccc
     2041 gagcgagcag ggccgcgctg gcttgctcac tcaagcgggc gtgatgtcgg tgcaagctca
     2101 tccggaccaa acctcgccgg tgctgcgcgg caagttcgtc cgcacgaagc tcttgtgcca
     2161 gccgccacct ccgccgcctc ccgacgtcga gatcaaggtg cccgacgcct cccctgggag
     2221 caccgcgcgg gatcgcttcg gcgcgcacct gcaggcggga accacctgca acggttgcca
     2281 taccttgatg gatccgatcg gttacgcctt cgagaacttc gacgcgctcg gcgagttccg
     2341 cacgacggag gccaacaaac cgatcgacgc gagcggcgag gtgatcgcga gcgcagaccc
     2401 cgcgctcggc ggcaagttca cgggcgtgcg agagctcgcc aacaagctgg ttcaaagtga
     2461 cctcgtgcgc gactgcgtcg cgacgcaatg gttccggttc gctgcgggcc gtttcgaagc
     2521 cgaaggcgat gcttgctcgt tgtcgaccct acaggagcgt ttcgcgagct cgggcggcga
     2581 cctgacggag ctcgtggtcg gcctcacgca gacggactcg ttctggtacc gcagccccta
     2641 gcagctacag gaatgatgcg atgaatccca tgaggtttac acgtcggaac ttgctgcgcg
     2701 gcggacttgc cgcgaccgct gccatcccgc tgctcaacgc gcagcgcgtg gaagggcagt
     2761 cggcgcagcc cattcgtcga ttcgtggtct tcacgactcc aaacggaacg cgcaactcgc
     2821 tgttctggcc gaccggcagt gagaccagct acacgctccc cgccatcacc aaggatctcg
     2881 agccgctcaa gagcaagctc ttgttcttga aaggcatcaa gctgaacgac gccctgcaga
     2941 acggggcact cggcgggacc gtcggctccg agcacgctcg cggcacgggc ggcatgctca
     3001 ccgcgcgccc
//
LOCUS       DASTKS010001097         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_394280, whole genome shotgun sequence.
ACCESSION   DASTKS010001097 DASTKS010000000
VERSION     DASTKS010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394280"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFQ35_23125"
     CDS             complement(<1..232)
                     /locus_tag="VFQ35_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9933625.1"
                     /translation="MKLYRFRYSPYARKVQMVLDLLGTDYEIIEVPFTDREDLAKLTG
                     GYIYVPVLQDEDGRVIVDSRAICERLLSDPAAR"
     gene            289..1302
                     /gene="gcvA"
                     /locus_tag="VFQ35_23130"
     CDS             289..1302
                     /gene="gcvA"
                     /locus_tag="VFQ35_23130"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HET9933626.1"
                     /translation="MSKTHDTLERALNRHGTTHVTPNLPSLNALLAFDSAARHESFTR
                     AARELGVTQTAVSHQIKALEAELGVLLFRRSPKRLSLTEDGLAWSAELRVIFQRLRDA
                     NVRLRSRSRSERPVVSVTTIPSFGARWLVPRLGKFMTQHADIDVRISANEQLVDFGLE
                     PFDLGIRYGSGRYSGLVTEKLAEDAWVVVCAPGLPGRNRLKTPADLAKVPLLCDDHPD
                     AWSAWFGATSRECPAFTARSEYTDSSMLVEAALRGQGVALARWSLACDELENGRLELP
                     FPKEPPVRTGRAYYLVGPREAFRRPPVAAFRDWLRVEARGLRTFGGSSSSTRHMYQLA
                     TDR"
     gene            complement(1365..1874)
                     /locus_tag="VFQ35_23135"
                     /pseudo
     CDS             complement(1365..1874)
                     /locus_tag="VFQ35_23135"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine methyltransferase"
     gene            complement(1871..2494)
                     /locus_tag="VFQ35_23140"
     CDS             complement(1871..2494)
                     /locus_tag="VFQ35_23140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9933627.1"
                     /translation="MPDPAGALPFGAMKSAAYSLFQTPIGACGIAWTANGVSVLQLPE
                     ARSSATRARVLARSPGALASDLRGEMREVVEAVRALLEGERRDLSGIELDYEGVPEFH
                     RRVYVAARRVLPGDVVTYGELARRMHAFGAARAVGQALGRNPFAIIVPCHRVLASGGK
                     VGGFSANGGASTKLRLLAIEGALGAVRGAGARGPAQQSLYEPRGTSR"
     gene            2567..3718
                     /locus_tag="VFQ35_23145"
     CDS             2567..3718
                     /locus_tag="VFQ35_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9933628.1"
                     /translation="MTLPRPLVPVLAVLGISAVAGLACKRSAGAPGVSSAHSLPSVVP
                     PPPSAEPIPPLSAGAYIEDERNTITVFRETAASAVFVTQKQVMVDYWAGQAVEVPAGS
                     GSGFIWDDQGHVVTNFHVVQNARSLTVTLQDQSTHEAILRGVEPRKDIAVLKIDVPKE
                     KLVPIRLRDNKKQSLEVGQKTIAIGNPFGLDHTLTVGVVSALGRSVDGVGGVTIRDMV
                     QTDAAINPGNSGGPLLDSGGRLIGMNTAIFSKSGVSAGVGFAVPASTIQRVVPQIIRT
                     GHAEQVGLGIRIDVEQRFERRAGVRGVVVLGVQPGSPAEKAGLKGVERTIRGAVLGDI
                     IVGVDGKPIDDYDDLYNALDAHQAGDQVKLKVRRGDQLVELTSALIAIQ"
     gene            3751..>3969
                     /locus_tag="VFQ35_23150"
     CDS             3751..>3969
                     /locus_tag="VFQ35_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9933629.1"
                     /translation="MDTDDALLSRSDDVIARLVGNHRQFLAFLESRLGNRKDAEDLLQ
                     AAFVKAIEKVDTLRDDESAVAWFFRLLRN"
BASE COUNT          701 a         1330 c         1276 g          662 t
ORIGIN      
        1 gtcgggccgc gggatcgctg agcaggcgct cacagatcgc acgcgaatcc acgatcacgc
       61 ggccgtcctc gtcctgcagg accggaacgt agatgtagcc gccggtgagc ttcgcgaggt
      121 cttcccggtc cgtaaaaggc acttcaatta tctcatagtc tgtccccagc aaatccagca
      181 ccatctggac ctttcgagcg tacgggctgt agcgaaaacg gtagagcttc atggtcaatc
      241 caatacgccg cttcggtctt tcggaaaaac gatactttcc catgatacat gagcaaaact
      301 catgatactc tggaacgcgc tctcaaccgc cacgggacga cgcacgtgac cccgaaccta
      361 ccctcgctga atgcgttgct cgcattcgac tccgccgccc gacacgagag cttcacgcgg
      421 gccgcgcgcg agctcggcgt cacgcagacg gcggtcagtc accaaatcaa ggcgctcgaa
      481 gcggagctcg gcgtgctgct cttccgtcgc tcgcccaagc ggctcagcct caccgaagat
      541 gggctcgcct ggtccgccga gttgcgcgtc attttccagc gtctgcgcga cgccaacgtg
      601 aggcttcggt cacgttcacg cagcgagagg ccggtcgtgt cggtgacgac aatcccgtcg
      661 ttcggcgcgc gctggctcgt cccacgtctc ggaaaattca tgacccaaca cgccgacatc
      721 gacgtgcgca tctccgccaa cgagcagctc gtcgattttg ggctcgagcc gttcgatctc
      781 ggtattcgct acggaagcgg ccgctactcc ggcctcgtca cggagaagct cgcggaagac
      841 gcttgggtcg tcgtctgcgc gcctggcctc cctgggcgca atcggctgaa gacgccggcc
      901 gatctggcca aagttccgtt gctttgcgac gatcacccgg acgcgtggag cgcctggttc
      961 ggcgcgactt cccgcgagtg cccggcgttc acggcgcgaa gcgaatacac cgactccagc
     1021 atgctcgtcg aggcggcgct gcgcggccaa ggcgttgccc tcgcgcgctg gtctctcgcc
     1081 tgcgacgagc tcgaaaacgg acggctcgag ctcccgttcc ctaaggagcc tccggtccgc
     1141 acggggcggg cctactactt ggtggggccg cgggaggcct tccgacgacc tcctgtagcg
     1201 gcttttcggg attggttgcg cgtcgaggcg cgcgggcttc gcactttcgg cggctcgagc
     1261 tcgagcacac gccacatgta ccagctcgcg acagaccgat agggtcgcca gcgatgcgcg
     1321 tgctcttcga gctgcttggc cgtgatcggc gccgatccgt ggaaggcgac cgaaaatccc
     1381 tttcgcacac cgaagtcgtg cagaggaagc acgtcgggcc gccccaacct gaacatgagc
     1441 agcatgtgca ccgtccagga tccgatgccg cgcacgcagg tgagcgtcgc gatgatgtcc
     1501 tcgtcgctca tttcctgaag ggcagagaac gaaggaagcg cgcccgaatc gaccttctcg
     1561 gcgaggtcgc gcagcgccgc gagtttgccg cgcgacaacc cagccgcgcg gagtgcctcg
     1621 tccggaatgc gcttgagcgc gcgcgcgctt ggcgctgaag ggcgcggaaa gagctggcaa
     1681 acgcgcgcga agatgctcgc agccgctttg gcgtgcagct gctggtacac gatcgcctct
     1741 gccaacgcgg caaagaggct gctcgtcggc ttgaggacga gccggtaagg gccgacgttt
     1801 tcgatgaccc cggcgagcac tgggtcttgc tcccccaagt gctggagcgc cgcgcgcacc
     1861 tgcgctggct tcaccgcgag gttccccgtg gctcgtacaa gctctgctga gccgggccgc
     1921 gcgctccggc tcccctgacg gcgccgagcg ctccctcgat cgcgaggagc cggagcttcg
     1981 tcgacgcgcc gccgtttgca gagaacccac ccaccttgcc gccgctcgcc agcacgcggt
     2041 gacagggcac gatgatcgcg aacgggtttc taccaagcgc ctgtcccacc gcgcgcgccg
     2101 cgccgaacgc atgcatccgg cgcgctagct cgccgtaagt gaccacgtcg ccgggcagga
     2161 ctcggcgcgc agcaacgtac acgcgacgat ggaactcggg gacgccctcg tagtcgagtt
     2221 cgatcccaga aagatcgcgg cgctcgcctt cgagcagggc tcgaaccgcc tcgaccacct
     2281 cgcgcatctc tccccgaagg tccgacgcga gcgcccccgg cgagcgggcg aggacacgcg
     2341 cacgggtggc ggatgagcgc gcctcgggaa gctgcaggac ggagacgccg ttggccgtcc
     2401 aagcgatgcc gcacgcaccg atgggggtct gaaacagcga gtaagccgcg gatttcatcg
     2461 cgccaaaagg tagcgcaccc gcgggatctg gcatggttaa acttgcgcca atcaggccgc
     2521 acagcgtcgt gcggtgtgag ctcgagctgc gctatgctcc gccgccatga cgcttccccg
     2581 cccgctcgtc cccgtgctcg ccgtcctcgg aatctccgcc gtcgcagggc tcgcctgtaa
     2641 gcgttcggcc ggtgcccctg gcgtgagctc cgcgcactcg ctgccgagcg tagtccctcc
     2701 gcccccgagc gccgaaccca tccctccgct atccgccggc gcgtacatcg aagacgagcg
     2761 caacaccatc accgtgttcc gcgagactgc cgcatcggcg gtgttcgtga cccaaaagca
     2821 ggtgatggtg gactattggg cggggcaggc ggtcgaagtc cccgcgggct caggctctgg
     2881 cttcatttgg gacgaccagg ggcacgtcgt gacgaacttc cacgtcgtac aaaacgcgcg
     2941 cagcttgacc gtgacgctcc aggaccagtc cacccacgag gcgattttgc gcggtgtcga
     3001 gccacgcaag gacatcgcgg tcctcaagat cgacgtcccg aaggagaagc tcgtcccgat
     3061 tcggttgcgc gacaacaaga agcagtcgct cgaggtcggc caaaaaacca tcgccatcgg
     3121 taacccgttc ggcctcgatc acacgttgac ggtcggggtg gtgagcgccc ttgggcgctc
     3181 ggtagacggc gtcgggggcg tcacgattcg cgacatggtg caaacggacg ccgccatcaa
     3241 ccccggcaac tccggcgggc cgctcctgga ctcgggcggt cgcttgatcg gaatgaacac
     3301 ggcgatcttt tcgaagagcg gggtgtccgc gggagtcggc ttcgcggtac cggccagcac
     3361 catccagcgg gtagtgccgc agatcatccg gaccggacac gccgagcagg tcgggctcgg
     3421 gatccgcatc gacgtagagc agcgcttcga gcgacgcgca ggggtccggg gagtggtcgt
     3481 gctcggtgtg cagcctggaa gccctgccga aaaagcgggt ttgaagggcg tcgaacgcac
     3541 gattcggggc gcggtactcg gggacatcat cgtcggcgta gatggaaagc cgatcgacga
     3601 ctacgacgat ctctacaacg ccctcgacgc gcaccaggca ggcgatcagg taaagctcaa
     3661 ggttcgtcgc ggggaccagc tggtcgagct cacctccgcg ctgattgcca tccagtagcg
     3721 ctcgcccgag cgggtgttag gcttcgcgac gtggacacgg acgacgcatt gctcagccgt
     3781 tccgacgacg tgatcgcgcg gctggtcggc aaccatcggc agtttctcgc ctttctcgag
     3841 tctcgcctgg gaaatcgcaa agatgcggag gacttgctcc aagctgcgtt cgtcaaagcc
     3901 atcgagaaag tcgacactct gcgagacgac gagagcgcgg tggcctggtt ctttcgctta
     3961 cttcgcaac
//
LOCUS       DASTKS010001098         7593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1289823, whole genome shotgun sequence.
ACCESSION   DASTKS010001098 DASTKS010000000
VERSION     DASTKS010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289823"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1314)
                     /locus_tag="VFQ35_23155"
     CDS             complement(52..1314)
                     /locus_tag="VFQ35_23155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9933630.1"
                     /translation="MSQPEVLPDAASGPRLHDANGTFRSLRGFNYRTWAIGALVSNVG
                     TWMQRTAQDWLVLTELTNRSGTAVGALMALQFGPQALLLPFTGYAADRLDRRKLLFVT
                     QASAGVLALGLGLLTLFGVVRLWHVYVFGLLLGCVSAFDGPARQTFVSELVSDEQLSN
                     AVALNSTSFNVARLLGPAAAGVAISRFGTGWVFVLNALSFAAVLVSLARLRNSELHAR
                     ERATKRAGLTEGFRYVRQRPDLAAALGMLFLIGTFGLNFPIFISTMAVSVFHKGAGQF
                     GLLTSIMAVGSVAGALLSARRAAPRVELLMAGAFAFGAGCALAAVMPTYGWFGFTLAV
                     IGVSAQTFTTTANGTVQLATDPHMRGRVMAIYMAIALGGTPIGAPIVGFVADRFGPRW
                     ALGVGAFAGFAAALVGFRYLARRTLKGA"
     gene            1415..1870
                     /locus_tag="VFQ35_23160"
     CDS             1415..1870
                     /locus_tag="VFQ35_23160"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9933631.1"
                     /translation="MARRSPEETLDRFGDVTRGLRLLSGQAYATFDVGTTQAKFLRYL
                     GQHQPLSQAELARATVTDPTLTGRVVQSLIERGWVDRDVSREDRRQYVLSLTAAGQRV
                     RKRVEAARRRVAERLVAALDDRDLADFDRIAQKLKAALASAADADAEPT"
     gene            complement(1890..3848)
                     /locus_tag="VFQ35_23165"
     CDS             complement(1890..3848)
                     /locus_tag="VFQ35_23165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9933632.1"
                     /translation="MSKEWQPGTGERGGNEASDSARYSADEHLVRAIFDGALDAMVLT
                     DEKGAFVNCNAAACELLRTPEKSLLGRRIAEFTAPGYDAVAVGQRLRKAGSLRGKFGL
                     RRADGVVRVVEFAAVANIVAGRNLAVMRDVTDRERSEAHQNLLAAIVDSSHDAIVSKD
                     LSGKISSWNRAAEELFGYTAEEAIGRNILMLFPEARRHEEPEIIARVMSGGKIDQFET
                     VRLHKDGTPIEVSLTISPVLDATGRVVGASKIIHDLRPRRRSETALRSMEEQLRQAQK
                     MEAVGQLAGGVAHDFNNILSVVLSYCQMILSAGTPGDPVLRDVQEIQRAGIRASELTR
                     QLLAFSRQQILKPRVLDVNAVVNGIFPMLRRLLGENIEVTVLLDENLWSVRADPGQLE
                     QVIMNLVVNARDAMPNGGKLTVETSNTNLDGSYASVHLGVLPGRYVLLAVTDTGHGMD
                     LATRERVFEPFFTTKEKGKGTGLGLSTVFGIVKQSGGNVWVYSEPDRGTTFKVYLPAT
                     DAEPDGPASVEQYSGNLRGSETILLVEDDDQVRSVIRSFLRREGYTVLEAQNGGEAFL
                     LVEQFSSKIDLLVTDVVMPRVSGRQVAERIRALRPNIRVLYVSGYTENSVVHHGILDS
                     GIAFLQKPVTPEALLRKIREVLDAPITH"
     gene            4940..7345
                     /locus_tag="VFQ35_23170"
     CDS             4940..7345
                     /locus_tag="VFQ35_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9933633.1"
                     /translation="MRTTSGLFSVRLALCVSVLAACGSQVGGGDRVGGGNGESGGAGA
                     TSSAAGGGGGLQGQGGLVILGLGGAQPKPSCDSDNPPPDCPMAKPGCGDGKINAAGED
                     CDDHNSLSGDGCSGICKVEPFHHCPTPGQPCVSDVVCGDGIVGPGEACDDKNTVSGDG
                     CAANCGLVEKGFNCRTPGQPCARVYVCGNGIVDPNEGCDDGKRVDGDGCDSKCRVETG
                     YKCDGMPSKCSTTKCGDGKKEGAESCDDGNLVPFDGCDSKCQAEPACTTGQACTSKCG
                     DGIILGTEQCDDGNQRDGDGCSHECKVESDQGFTCKDVTTACVQDANGKCALRVPAIF
                     RDFKGRNESGGHPDFEQGGTNKLTITGLVKKDLDGDGKPVYAGGTNAALIQSVDSFAQ
                     WYRTSNVNKELVGEIVLWDNGKGGFVNRFGPNGEQFKGWPSTVAGNPAIETPGVNKPV
                     PQVYQCLESRTIDPVTMMEVVDKSCASDPLCAAPGPDQECEERCLGYGDVQNFPCLSA
                     IQYFDGQPLFFPVDSLAAGTQYGQVTPAYAMNWKSEETIAQVTMPRKHNFSFTSEVKY
                     WFKYVASATPSQLDFTGDDDVWVFINGHLAVDIGGYHIPQNGSVTLDRTRAQALGLED
                     GKVYQIAIFHAERQTKASSFRLTLAGFSAARSDCGAICGDGKIAPGEECDDGTDKNTG
                     GYDHCGSDCTLGPRCGDHVLQADSGEVCDEGVDGNNGSYNGCAANCQPGPRCGDSVVQ
                     SQNEQCDDGKNTGDYGTCNMDCTLAPHCGDHIVQADHEECDDGNNASGDGCSAACTRE
                     ILR"
BASE COUNT         1428 a         2480 c         2471 g         1214 t
ORIGIN      
        1 tggcggccgt gttcggcatc acgaggcggc gccgaacgcg ctgacgcaac ctcacgcgcc
       61 tttcagagtc cgccgcgcga ggtaacgaaa ccctaccagc gcggcggcaa accctgcgaa
      121 cgcgccgacc ccgagcgccc agcgtgggcc gaaccggtcc gcgacgaacc ccacgatagg
      181 cgcgccgatg ggggtgccgc cgagcgcgat cgccatgtag atcgccatca cccggccgcg
      241 catgtgcggg tcggtcgcga gctgaacggt gccgttcgcg gtggtcgtga aggtttgagc
      301 cgaaacgcca atgacggcga gcgtgaagcc gaaccagccg tacgtgggca tgaccgccgc
      361 gagcgcgcac cccgcgccga acgcgaacgc tcccgccatg agcaactcga cgcgaggcgc
      421 cgcccgtcga gcagaaagaa gcgcgcccgc gacggagccg acagccatga tcgacgtgag
      481 gaggccgaat tgtccggccc ccttgtgaaa gacgctcacc gccatcgtcg agatgaagat
      541 gggaaagttg agcccaaacg tcccgattag aaagagcatg ccgagcgcgg cggcgaggtc
      601 cggccgctga cgcacgtaac ggaagccttc ggtcagccca gctcgtttgg tcgcgcgctc
      661 tcgagcgtgg agctcggagt tgcgcaggcg cgccaacgag acgagcacgg ccgcgaacga
      721 gagcgcgttc agcacgaaga cccagcccgt accgaagcgc gaaatcgcga ccccagccgc
      781 agccggaccg agcaggcgcg caacgttgaa cgaggtcgag ttgagcgcga cggcgttgga
      841 caactgctcg tcgctcacca gctcggagac gaaggtttgc cgcgcggggc cgtcgaaagc
      901 gctcacgcag ccgagcagca gaccgaacac gtagacgtgc cacaaccgaa cgacgccgaa
      961 caacgtgagc aagccgagcc ctaacgcgag cacgccggcg ctcgcctgcg tgacgaacag
     1021 gagcttgcga cgatccaagc gatctgcggc gtagccggtg aacggcaaga gcagcgcctg
     1081 cggcccgaac tggagcgcca tcagtgcccc gactgcagtg ccgctgcgat tggtgagctc
     1141 ggtcaggacg agccaatcct gggcggtgcg ttgcatccag gtgccgacgt tcgagacgag
     1201 cgccccgatg gcccacgtcc tgtagttgaa gccccgcaat gaacgaaaag tgccgttcgc
     1261 atcgtgcagg cgagggccgc tggccgcgtc gggaaggacc tcgggttggg acattactag
     1321 aggggtctac tattaaagat agacccgtcc atcaaaccgt caagcggtcc ggttttccca
     1381 ttgcgagcgc cgacacaccg gctaaattca tcgcgtggca cgaaggagcc cggaagagac
     1441 gctcgacaga ttcggcgacg tcacccgcgg gctacgcctg ctctcggggc aggcttacgc
     1501 gacgttcgac gtaggcacca cgcaagccaa gttcttacgc tacctgggcc agcatcagcc
     1561 tctctcacaa gccgagctcg cgcgcgccac cgtaaccgac ccgactctca ccgggcgcgt
     1621 cgttcagtcg ctcatcgagc gcggctgggt cgaccgcgac gtgagccgcg aggatcgtcg
     1681 tcagtacgtg ctctcgctca ccgccgcggg gcaacgcgtc cgaaagcggg tggaggccgc
     1741 gcgccgccga gtggccgagc gcctcgtcgc ggccctcgac gaccgagatc tcgccgactt
     1801 cgatcgcatc gcgcaaaagt tgaaggcggc gctcgcgagc gcggcagacg ccgacgcgga
     1861 gccgacatag ccggcacaag tcaccgcgct caatgcgtga tcggcgcatc gaggacttcg
     1921 cggatcttcc tgagcagcgc ctcgggcgtg acgggcttct gcagaaacgc gatgccggag
     1981 tcgaggatgc cgtggtgcac caccgagttc tcggtataac cggaaacgta gagcacgcgg
     2041 atgttcggtc gcagcgcgcg gatccgttcg gcgacttgcc gaccgctcac gcggggcatc
     2101 acgacgtcgg tcacgagaag gtcgatcttc gagctgaact gctcgacgag cagaaatgct
     2161 tcgccgccgt tctgcgcctc gagcaccgtg tagccttcgc gacggaggaa agagcggatg
     2221 accgaacgca cttggtcatc gtcctccacg aggaggatgg tctcgctccc gcgcagattg
     2281 ccgctgtact gctcgacgct cgcgggcccg tccggttcgg cgtcggtcgc gggcaggtac
     2341 accttgaacg tggttccccg gtccggctcg ctgtaaaccc agacgttccc gccgctctgt
     2401 ttgacgatgc cgaacaccgt cgaaaggccg aggccggtac ctttcccctt ctctttcgtg
     2461 gtgaagaacg gctcgaatac ccgctcacgc gtggccaaat ccatgccgtg cccggtgtcg
     2521 gttacggcga gcaagacata gcgtccgggc agtacaccga ggtgaaccga agcgtagctg
     2581 ccgtcgagat tcgtgttgct ggtctcgacg gtcagcttgc cgccgttcgg catcgcgtcc
     2641 cgcgcgttga ccacgaggtt catgatcacc tgctccaact gccccgggtc tgcgcgcaca
     2701 ctccagaggt tctcgtcgag caagacggtg acctcgatgt tttctcccaa gagacgccga
     2761 agcatcggaa agattccgtt caccaccgcg ttcacgtcga gcacccgcgg cttgagaatc
     2821 tgctgtctgc tgaaggcgag gagctggcgc gtgagttcgc tggcgcggat cccggctcgc
     2881 tgaatctctt gaacgtcacg cagtacggga tctcccggcg tccccgcgct caggatcatt
     2941 tggcagtagc tcaagacgac gctcaggatg ttgttgaagt cgtgggccac gccccctgcg
     3001 agctggccaa ccgcttccat cttctgtgct tgccgtagct gctcttccat gctccggagt
     3061 gcggtttccg agcgccgccg aggtcgcaag tcgtggatga ttttcgaggc tcccaccacg
     3121 cgccccgtgg cgtcgagcac tggcgaaatc gtcagggaga cttcgatggg ggtgccgtct
     3181 ttgtgcaggc gcaccgtttc gaactgatcg attttgccgc cgctcatgac ccgagcgatg
     3241 atctccggct cctcgtgccg tctcgcttcg ggaaaaagca tcaggatgtt gcgtccgatc
     3301 gcttcctccg cggtgtagcc gaagagctct tccgcggcgc ggttccagct cgagatcttg
     3361 ccgctgagat ctttgctgac gatcgcgtca tgggacgagt cgacgatggc ggccaagaga
     3421 ttttggtgcg cctcgctccg ttcgcgatcc gtcacgtcac gcatgacggc gaggtttcgc
     3481 cccgcgacga tgttagcgac cgcggcgaac tcgacgacgc ggacgacgcc atccgctcgc
     3541 ctgagcccga atttcccgcg taaggagccg gccttacgga gccgctgacc cacggccacc
     3601 gcgtcgtacc ccggagccgt aaactcggcg atgcgtcgtc cgaggaggct cttttcaggc
     3661 gtccgcaaga gctcgcaggc agcggcgttg caattcacga acgcgccctt ctcgtcggtg
     3721 agcaccatcg cgtcgagagc tccgtcgaag atggcccgta ccaagtgttc gtccgctgag
     3781 tagcgcgcgc tgtcgctcgc ctcattgccg ccccgctcgc cggtaccggg ctgccactct
     3841 ttggacattc tttacaagcg tagcaacggt tcagcgaatc tcttcgcaaa ccgcggattg
     3901 aagttcatgt tcgcgcacgc gcgcacctcg tgaagtgcaa acatgccccg ctctgtccgg
     3961 gatcttcccc gacagaggtg acgtaacccg ccggtacgag cgccagagga ctcacccacc
     4021 ggccacgttc ccatgcgagg cgatgacgat ctcgcgtcgt gacgcacgca tccagcatgc
     4081 gttcgcagca gcgctcaccg cggcgcgacc gaggtcactg acgtacgtag acgagtgtga
     4141 ccacgcgacg aggctcctgg cacgagcgat tcacgcaggc tgatcttctc accgcgctcg
     4201 cggtcacgaa cgagtcgaca agatgagaag gtcactcggt tgcctccgag aggagcaccg
     4261 acgcgcgcgg gtgcggattc ttgaccaaga ttggcgtcgc cacgcggagg cagacggcaa
     4321 gtccgactgg tcgtgtccgg aatggctcac cttcgttcaa cggtgcgcga agcgggccga
     4381 cgaaaaggct tcgccgtcac gcgcccccga aagccccgac ctcgtacggc gcggccgacg
     4441 acgctgggat cagtcgccca ctttcggcgc ggcgccatgg ccgcagccga tctccccgcc
     4501 ttgcgctcgg ccgcttccac ccggaaccgc cgtttggagg actgagaggg ggcgaggctg
     4561 tccacgtgcg ctctcggctt aatctgccaa agtccgaact ttgctgcgga gctgccgagt
     4621 cgcccggtcc ttcgagacgc tccctccctc gtggccaggt gctcgcttca attttcgtgt
     4681 gtcacagcac gcggcgcggg gaagctcgct tcgcggagcc gagatccacg cggtgcggac
     4741 cgagttcccg gcgccgcccg cactgatttc tgcatgctgg ccgcgcgctc gccgcactag
     4801 ctggttgcgg gcgcgagtaa cgtcgcgcca aacaaggtga tagagtacgt cgaaagcgac
     4861 ggcggcattg tccgcgtgtc gcgcttgctt tggtaggacg ggcgcgtgca gcaatcaggc
     4921 gtcctttgag gtttttacaa tgcgaacgac cagcgggctt ttttcagttc gcctcgcgct
     4981 gtgcgtcagt gtgcttgcgg cgtgcggctc ccaagtgggt gggggtgacc gcgtcggcgg
     5041 cggaaacggg gaaagcgggg gcgcaggagc gacgagctcg gccgctggcg gcggcggcgg
     5101 actgcaaggg caaggcgggc tggtcatcct cgggttgggc ggcgcgcagc cgaagccaag
     5161 ctgcgactcc gacaacccgc caccggattg tcccatggcc aagccaggct gcggcgacgg
     5221 caagatcaac gcggccggtg aagactgtga cgatcacaac agcctctcgg gcgatggctg
     5281 ctccggcatt tgtaaagtcg agccgttcca ccattgccca actcctgggc agccctgcgt
     5341 ttcggacgtg gtttgcggcg acggcatagt cggccccgga gaggcgtgcg acgacaagaa
     5401 caccgtctcg ggagatgggt gcgctgcaaa ctgcgggctc gtcgaaaagg gcttcaactg
     5461 ccggactcca ggacaacctt gcgcgcgcgt ttacgtctgc ggcaacggca tcgtcgaccc
     5521 caacgagggt tgcgacgacg gcaagagagt cgacggcgac ggttgcgact cgaagtgcag
     5581 ggtcgagacc ggctacaagt gtgacgggat gccgagcaag tgctcgacga ccaagtgcgg
     5641 cgacggcaag aaggaaggcg ccgagagctg cgacgacggc aacttggttc cgttcgacgg
     5701 ctgcgactcg aagtgtcagg cggagccggc gtgcaccacg gggcaagcgt gcacgtccaa
     5761 gtgcggcgac ggcatcatcc tcggaaccga gcagtgcgac gacggtaacc aacgcgacgg
     5821 cgacggctgc tcgcacgagt gcaaagtgga gtccgaccag ggcttcacct gcaaagacgt
     5881 caccacggcc tgcgtgcaag acgcgaacgg gaagtgcgcg ctgcgcgtgc cggcgatctt
     5941 ccgtgacttc aagggcagga acgagtccgg cggccacccc gacttcgagc agggcggaac
     6001 caacaagctc acgatcactg ggctcgtcaa gaaagacctc gatggcgatg gcaagcccgt
     6061 gtacgcgggc ggaaccaacg ccgccctgat ccaaagcgtc gactcgttcg cgcaatggta
     6121 ccgcacctcg aacgtcaaca aggagctcgt gggcgagatc gtactctggg acaacggcaa
     6181 aggcggcttc gtcaaccgct tcggtcccaa cggcgagcag ttcaagggat ggccatcgac
     6241 cgtcgccggc aatcccgcga tcgaaactcc cggcgtgaac aagccggtcc cccaagtcta
     6301 ccagtgcttg gagtcgcgca cgatcgaccc ggtcacgatg atggaggtcg tggacaagtc
     6361 gtgcgcttca gatcctctgt gcgcggcgcc cggcccggat caagagtgcg aggaacgctg
     6421 cctcggctac ggggacgtcc aaaacttccc ctgcctgagt gcgattcagt acttcgacgg
     6481 ccagcccctg ttcttccccg tcgactcgct ggcggcgggg acccagtacg gacaggtcac
     6541 gccggcctac gcgatgaact ggaagagcga ggagaccatc gcgcaagtga cgatgcctcg
     6601 caaacacaac ttcagcttca cgagcgaagt caaatactgg ttcaagtacg tggcatcggc
     6661 gacgccttct caactggact tcacgggcga cgacgacgtc tgggtcttca tcaacggcca
     6721 cctggcggtg gatatcggcg gctaccacat cccgcagaac ggctcggtga cgctcgaccg
     6781 aacgcgcgct caagcgcttg ggctcgagga cggcaaggtc taccaaatcg cgatttttca
     6841 cgctgaacgc cagaccaaag cgtcttcctt ccgcctgact ctcgccggct tcagcgccgc
     6901 gcgcagtgac tgcggcgcga tctgcggcga cggcaagatt gcccccggcg aagagtgcga
     6961 cgacggcacc gacaagaaca cgggcggcta cgaccactgc gggagcgact gcacgcttgg
     7021 cccgcgctgc ggtgaccacg ttctgcaggc ggactctggc gaggtctgcg acgagggcgt
     7081 cgacggcaac aacggttcgt acaacggctg cgccgccaat tgtcagcccg gtccgcgttg
     7141 cggcgacagc gtcgtccaga gccaaaacga gcagtgcgac gacggcaaga acaccggtga
     7201 ctacggcacc tgcaacatgg actgcaccct cgccccccac tgcggcgatc acatcgtgca
     7261 ggcggaccac gaagagtgcg acgacggcaa caacgcatcc ggagatggct gcagcgcggc
     7321 gtgcactcgc gagatcttgc gctgagcgag gcgagcgccc ggcgcgatag gctcgacttg
     7381 cgtcgagacc gccgggcgcc actcgaacac cagcgcgagc tcgtaccgct cgttcaaagc
     7441 acggcgctcg gcgcaaccgc cgacggcgcc ggcgaaagct cgggttcgag ggctcgcgtc
     7501 ttcgaaggca gggctacgag cgcgcacgag cacgcgaaca tcacgagtac gagctcggca
     7561 agcatcgccg tcgcttgttg cgagtcgacg ggg
//
LOCUS       DASTKS010001099         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_618469, whole genome shotgun sequence.
ACCESSION   DASTKS010001099 DASTKS010000000
VERSION     DASTKS010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618469"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFQ35_23175"
     CDS             <1..631
                     /locus_tag="VFQ35_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933634.1"
                     /translation="EQARFDDSRIDWSENAVMRVLGGIERSLAETEYSHATFVNERRG
                     SYKFDCSGMVQWVLRKAAPQAARSTYVGPAERPLARDFQRRIARAPVDRARAGWRRVA
                     RVNELRPGDVIAWIKPAEIDSPNTGHTAFVVLPPVRVEGYENAYLVRIADSTRLMHDE
                     DTRAGRTGFGFGTIMLVADPDTGAPVAYGWVGLRWRAFATAISLGRPEA"
     gene            677..1261
                     /locus_tag="VFQ35_23180"
     CDS             677..1261
                     /locus_tag="VFQ35_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933635.1"
                     /translation="MVIRPLGFAFFLGSLVACGPHTDTQENGAAPPNTGTLPDGTSCG
                     PAPADAPGAAAAWQRFLDWHAANAAVCGNSEKWPTYECWIRTADPRSVQAFTACMMSD
                     GCSSISNEDACIANPQNPAAGYQLSGAAAAWYQNVCIPKAVKCGFSDDNCSVFVPALR
                     PELRCAVVDCVEGSCENFAACIKAVNTRFSTCSL"
     gene            1350..>3400
                     /locus_tag="VFQ35_23185"
     CDS             1350..>3400
                     /locus_tag="VFQ35_23185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9933636.1"
                     /translation="MAAALRALLRLPSGDRQPHYERVAAVAGVSLSTEPAEAEDTSAY
                     DLVIFDVPRGKGEWLDAALVRSRYPGSAILVVAESFAEVAAALRRSGASFCPYADPED
                     LVAALRARSSNRALDAAAPALEFEAAQLEIIEQIAMGKSLPETLERIVRLAEGRTEEG
                     LCTVLLLGEGERIVSGVAPSLPDTFANVYVGRVIGPEEGSCGAAMALQTGIFVRDIAT
                     HPNWVKYREVALSLGLKSCWSMPILSSNGGVLGAFALYYREPREPSEREVAWVQRASH
                     LAAIAVMRDRAEQALRASEARYRQIVDTAYEGVWSVDAQARTLFVNARAAELLGYSVE
                     EMLGKSLFDFMEPEARVLAEGQMLRRFQKISEQHEFKFRRKDGSPLWVLVAASPLLDE
                     RGVAAGALGMLTDITRLKHAEATLRDSEVELRTIFENAAIGIALVDEEGRPRRSNPAL
                     QKMLGYSGEELAKMRFAEFTHPEDAGSDLDSYRRMLSGEISSYQMEKRFIHRSGKIVW
                     GRLTASFVRVEGGALLGIGMVEDITAHKVAQARISAQAALLDQATDAILIRSAEGVIE
                     YWNRGAQRLYGWSSEEVVGRNVVDFLYGDPAPFLLAQKRLLAEGAWSGELVQQTQSGQ
                     QVTVECRWTLARDEGDGIGKVLAINTDVTEKKRLEAQIISAQRMESLGTLAGGIAHDF
                     N"
BASE COUNT          580 a         1058 c         1159 g          603 t
ORIGIN      
        1 ggagcaagct cgcttcgacg atagccgcat cgactggagc gagaacgcgg tgatgcgcgt
       61 cctcggcgga atcgaacgct cgctcgccga gaccgagtat agccacgcta ctttcgtcaa
      121 cgagcgacgc gggtcgtaca agttcgattg ctcgggcatg gtgcagtggg tgcttcgcaa
      181 ggccgcgccg caggcggctc gcagcaccta cgtaggcccc gcggagcgcc cgttggcacg
      241 cgactttcaa cgccggatcg cgcgggcgcc agtggaccgc gctcgcgcgg gctggcgccg
      301 cgtcgcgcgc gtgaacgagt tgcgcccggg tgacgtcatc gcttggatca agcccgcgga
      361 aatcgactcg ccgaacaccg gtcacacggc gttcgtggtg ttacctccgg tgcgcgtcga
      421 ggggtacgaa aacgcttatc tcgtgcgtat tgccgactcg acgcgcctca tgcacgacga
      481 ggacacgcgc gccggtcgta cggggttcgg cttcggtacc ataatgctgg tcgcggatcc
      541 ggataccggg gcgcccgtcg cctacggttg ggtcggcttg cggtggcgtg ccttcgcgac
      601 cgcgatttcc ctcggccgtc ccgaggcttg acgcgcgagt gccttggcac ggatccgtct
      661 gcggtatgat gcggacatgg tgattcgtcc gttggggttc gcgttttttt tggggtcgct
      721 cgtcgcctgc ggtccgcaca ccgacactca ggagaacggg gccgcgccgc ccaacaccgg
      781 tactctcccc gacgggacca gttgcgggcc agcgcctgcc gacgcgccgg gagctgctgc
      841 agcgtggcaa cgttttctcg actggcatgc ggcgaacgcc gccgtctgcg gcaactcgga
      901 aaagtggccg acgtacgagt gctggatccg gacggcggac ccccgaagcg tgcaggcgtt
      961 tacggcgtgc atgatgtcgg acggttgcag ctcgatctcc aacgaggacg cctgcatcgc
     1021 gaacccgcag aacccggctg ccgggtacca attgagcggg gctgccgccg cttggtatca
     1081 aaatgtttgc atcccgaagg ccgtgaagtg tgggttctcc gatgacaatt gctctgtctt
     1141 cgtgcctgct cttcggccgg agttgcgctg cgccgtcgtg gattgcgtcg aggggagctg
     1201 cgaaaacttc gcggcctgca tcaaggcggt gaacactcgc ttttcgacct gttctctatg
     1261 agcaagcgtt tgggggtgcc tcgcgctgcg tctcgggacg gcggtcgtat cgccatgcgc
     1321 gagtgcgcgt ggtatcgagc atgaaggtca tggcggctgc tctccgcgcc ttgctgcgtc
     1381 tcccgtcggg agaccgtcag ccccactacg agcgcgttgc cgccgttgcc ggcgtgtctc
     1441 tgtcgaccga gccggcagag gctgaagaca cgtccgcgta cgacctcgtg atcttcgacg
     1501 tcccgcgcgg aaagggagag tggctcgacg ccgcgctcgt gcgctctcgg taccccgggt
     1561 cggcgatctt ggtagtggcc gaatcgttcg ccgaagtcgc cgctgccctg cgtcgaagtg
     1621 gggcttcgtt ctgcccctac gcagacccag aagacctcgt cgcggcgctg cgagcgcgca
     1681 gctcgaatcg cgctctcgac gcggccgcac ccgctctcga attcgaggcg gctcagctcg
     1741 aaatcatcga acagatcgcg atggggaaga gccttcccga aacgctcgag cgcatcgtgc
     1801 gactcgcgga ggggaggacc gaggaagggc tctgcacggt actactgctc ggcgaaggcg
     1861 agcggatcgt gagcggcgtg gcgccgagct tgccagacac cttcgccaac gtttacgtcg
     1921 ggcgcgtcat cggtcccgaa gaaggctcct gcggagccgc gatggctctt caaacaggca
     1981 tcttcgtgcg cgacatcgcc acccacccga actgggtgaa gtaccgtgag gtcgcgctct
     2041 cactcgggct caagagctgc tggtcgatgc cgatcttgtc gagcaacggc ggtgtgctcg
     2101 gcgccttcgc tctttactat cgagagccgc gcgaaccgag cgaacgtgaa gtcgcgtggg
     2161 tgcagcgcgc gagtcacttg gctgcgatcg cggtcatgcg tgatcgagcc gagcaggcgc
     2221 tccgagcgag cgaggcgcgc taccggcaga tcgtcgacac cgcctacgaa ggggtctggt
     2281 cagtggatgc gcaggcgcgc acgttgttcg tcaacgcgcg cgcggccgag ctgctcggct
     2341 actccgtcga ggagatgctc ggaaaaagcc tgttcgactt catggagccg gaagcgcggg
     2401 tgctcgcgga agggcaaatg ctgcgtcgct tccagaagat cagcgagcag cacgaattca
     2461 agtttcgccg caaggacggc tctcccttgt gggtgctggt cgcggcgagc ccgctgctcg
     2521 acgagcgtgg ggtcgccgcc ggcgcgctcg gaatgctcac cgacatcacg cggctcaaac
     2581 acgccgaagc gacgctgcgc gacagcgagg tcgagctccg cacgattttc gagaacgccg
     2641 ccatcgggat agccctcgtc gacgaggagg ggcgtccgcg gcgaagcaac ccagctttac
     2701 agaagatgct gggatactcg ggcgaagagc tcgcgaagat gcgctttgcg gagttcactc
     2761 accccgaaga cgccggcagc gatctcgatt cgtatcgacg catgctgagc ggtgagatca
     2821 gctcctatca gatggagaaa cgcttcatcc accggagcgg caagatcgtg tggggccggc
     2881 tcacggcgtc gttcgttcgc gtggaggggg gcgcgttgct cgggatcgga atggtggaag
     2941 acatcaccgc tcacaaagtc gcccaggcgc gcatttcggc ccaagctgcg ctgctcgatc
     3001 aggcgacgga cgcgatcttg attcggagcg cggagggcgt gatcgagtac tggaaccgcg
     3061 gcgcgcaacg gctctacggg tggtcgagcg aagaggtcgt cgggcgcaac gtcgtcgatt
     3121 tcctctacgg cgatccggct cctttcttgc tggcgcaaaa gcgtttgctc gccgagggcg
     3181 cgtggagcgg agagctcgtt caacaaacgc agagtggtca gcaagtcacg gtcgaatgcc
     3241 gttggacgct cgctcgcgac gaaggcgacg ggatcggcaa agtcctcgcc atcaataccg
     3301 acgtcaccga aaagaagcgc ctcgaagcgc agatcatctc tgcccagcgc atggagagcc
     3361 tgggaaccct cgctggcggt atcgcgcacg acttcaacaa
//
LOCUS       DASTKS010001100        10269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_618630, whole genome shotgun sequence.
ACCESSION   DASTKS010001100 DASTKS010000000
VERSION     DASTKS010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618630"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..3759
                     /gene="nifJ"
                     /locus_tag="VFQ35_23190"
     CDS             229..3759
                     /gene="nifJ"
                     /locus_tag="VFQ35_23190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET9933637.1"
                     /translation="MDGNEAVASVAYRLNEVIGIYPITPATPMGEQCDEWAAHGRQNV
                     FGSVPTVVEMQSEGGAAGAVHGALQAGALSTTFTASQGLLLMIPNMYKVAGELLPCVI
                     HVAARSLATHALSIFCDHSDVMACRQTGFALLAARSAQQAHDFACIATLATQAARVPF
                     LHFFDGFRTSHELVRVAELDDDDLRSLLDEGLLAERRRNALSPDHPSVRGTAQNPDTF
                     FQAREAANRFYAACPEHVEATMARFAALTGRSYRLFEYSGHPEAERVVVAMGSALETI
                     AGTVEELVRRGEKVGVLGVALFRPFSVERFLAALPVTAKRIAVLDRTKEPGAIGEPLY
                     LDVVTALSEGDRSAGTRVIGGRYGLSSKELTPAMVKGVYDELARPNPKRHFSLGIHDD
                     VTFESLAFDEEFELDRGQLREALFFGLGADGTVGANKNTIKIIGDHGRFEVQGFFVYD
                     SKKSGAATISHLRFDEQPIRRPYLIERADFVACHQFELLDRLPVLDHVRDGGVLLINS
                     PFPPDATFNQLSREVQSAIIDKRLRVYVIDGYRVAVDAGLGKHINTVMQACFFALSDL
                     MPLEQSITAIKKGIEYSYAKKGAEVVRRNLEAVDAARTALRELTLPARVDDSARARRP
                     PVPDSAPDFVQRVTAAMIAGLGDRLPVSAFPIDGTWPSGTTRFEKRRLTTTAPVWDSK
                     LCIQCNKCVLVCPHAAIRAKVFEPQAAAGAPEGFQATPWKGHGFPGEQYSLAVAPEDC
                     TGCTLCVAACPAKDKSNPRRKALEMQTLSDSLDSERKTYDFFLNLPPVDRRRVRLDVK
                     GAGLIDPLFEYSGACSGCGETPYLKLLTQLFGDRLLLANATGCSSIYGGNLPTTPYAT
                     DSAGRGPAWANSLFEDNAEFGLGIRLGVDAHRRHARELLAELRTRLDGALVDALLAAP
                     QGDDNEIYAQRGRVNELRTALASIDDPRARRLSTLADYLVKKSLWIVGGDGWAYDIGF
                     GGLDHVLASNEDVNVLVLDTEVYSNTGGQRSKATPRAASAKFAALGKDTEKKDLGLHA
                     MSYGHVYVARVAFGAKDAQTLRAFSEAEAHPGPSLIIAYSHCIAHGYDMKFGLDQQSL
                     AVRSGVWPLFRFDPKRIAEGEPPLALDASPGNASVTEYMRGEGRFQGLERLDPARYEE
                     LKSAAELDVRRRIELYEALSHIHFSTAKKD"
     gene            3763..4776
                     /locus_tag="VFQ35_23195"
     CDS             3763..4776
                     /locus_tag="VFQ35_23195"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HET9933638.1"
                     /translation="MDLSASYLGLKLPHPLILGASPLVDDLDTVRSAEDAGAAAIVMH
                     SLFEERILMEAGAALSLESHEDTFAEALYFRPNRDRFALGPDEYLEKIRRIKDVVSIP
                     LIASLNGTTARGWLEYARLIDQAGADALELNVYRVAANADETAEGIEAETADMVRAIK
                     SKTNLAVAIKLSPFYTSLSNVVERLIDAGADGLVLFNRFYQPDFDLEALEVRHSLELS
                     SSSELLLRLRWLAILSAQLAPSVTLAASGGVHNAVDALKAVMAGAHGVQMVSEILHHG
                     VNRFAEVRRDMEEWLEKHEYESLAQARGSLNSTRCPDPSAYERANYVRVLGSWRGNAL
                     PAR"
     gene            4934..5350
                     /locus_tag="VFQ35_23200"
     CDS             4934..5350
                     /locus_tag="VFQ35_23200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9933639.1"
                     /translation="MSPSPIKRILVAVDLSACSRAALEFARRVAEPLGASLEALYVLS
                     PDHGAGHELELAAAREELHRFVHSVVGATPVVEHVEPGEPRERIVAVSDRGGFDAIVM
                     GTEGRTGRARAFAGSVAESVVRTASVPVMTVREKRH"
     gene            complement(5391..6677)
                     /locus_tag="VFQ35_23205"
     CDS             complement(5391..6677)
                     /locus_tag="VFQ35_23205"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9933640.1"
                     /translation="MSSSSNETSSVTSPLGGLTQDPAKKRGRERKRALARNVRHALLG
                     LAIVAAAAGAVVALRPAPVPVDAEPVKRGDLSVVIEEDGVTRVKDRFVVSAPVTGSLS
                     RLRLEAGDVVKEGDTLAEIAPAVSPLLDERTRAESEARLGAALSALGQARAQISRASV
                     AKELAEQELSRSRRLFASSSITAQALDESEFLARMRGEELSSAEFAGKVATEEVRVAR
                     VALGRDGAGSRDRHIDVLSPISGRILRVHQKSAGIVQAATPLVEVGNPGDLEVVVDLL
                     TTDAVRVLPGTAVLIRGWGGDHSIAGRVRRVEPSAFTRPSALGVDEQRVNVVVALTGP
                     EQSWSALGDGYRVETRIVLWQGSRVLKVPQGALFRHGESWAVFRVEGGAARLTPVSVG
                     HRGETEAEITGGIGEGALVAVHPGDRVKDGARVEQR"
     gene            complement(6677..9046)
                     /locus_tag="VFQ35_23210"
     CDS             complement(6677..9046)
                     /locus_tag="VFQ35_23210"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933641.1"
                     /translation="MFTRALDRKLLRETTRLKGQIATIALVLASGIICFIALRGTYIS
                     IAESRLVYYDRYRFAHVFAQAERVPETTARRLEEIPGVEVLQTRVSEDVTVPIEGMPR
                     PAYGRLLSLPAGRTPATNALFLRKGRLPEATHQDEVVLLESFAEAHGIDPGQRVPVVM
                     NGKRRELRVVGIALSPEFVYAIRPGAMVDDPKRHAVLWMERTALASAFQLGGAFNDVS
                     LRISPNASEQAVRTAVDRVLGPFGGTGSIGRKDQISNKIVSQELDQLGALSTMVPAVF
                     LAVAAFLVNLVLGRLIRLQRPEVATLKAVGYTNWEIGRHYLGLVAIVLVPGGVLGLLG
                     GLGLGRVVLGLYATSFRFPELRFRMTFALMASALLVSAIAALVGALGAVRSAVRMPPA
                     EAMQPPAPATYKRTWLERLGLGGLIGPSGTMVVREVARRPLRTLLSSIGIAGAVSLLI
                     FSHFGLDSILAYFEGTFLREQRQDVSVVFARPVSPRVVAELGRLPGVIRAEGIRAVPV
                     RVTFEHRSRESVLMGLPEASTLRRLVDRKRAEVPVLPDGIVLTKTLGDVLGLHVGDRP
                     QIELREGDRRSVRPVVLGFVDESIGLSIYAQTDELASLEGDTGAVSAVLLKIDRPFVD
                     TVNARLRESPHVIDISDVKGDMQRTLDMNASIMNVWTAVSVFLSASIVFGVVYNNARI
                     GLAARSRDLASLRVLGFTRREISTILLSSQVLEVAIAIPIGLWLGSVWARAFMRTVDP
                     EVFRWTVVVAPSTYLLSIGVTVLAAAASALWVRRSLDSLDLIAVLKARE"
     gene            complement(9051..9794)
                     /locus_tag="VFQ35_23215"
     CDS             complement(9051..9794)
                     /locus_tag="VFQ35_23215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933642.1"
                     /translation="MAAPAHTTSANEAPNSVLSCRALRKIYRMGEVDVRDLDGVDFEL
                     RRGELLVLLGPSGSGKSTLLNILGGLDVPTSGSVFFGTEDLTEADDDQLTAYRRHQVG
                     FVFQFYNLIPSLTARENVALVTDIADDPLEAEDALALVGLGERLDHFPTQLSGGEQQR
                     VAIARAVAKRPSVLLCDEPTGALDFRTGVRVLEVLERVNHELGTATAIITHNAPIAAM
                     ADRVVTLRDGLVESETSNPEPAAARALRW"
     gene            complement(10044..>10269)
                     /locus_tag="VFQ35_23220"
     CDS             complement(10044..>10269)
                     /locus_tag="VFQ35_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933643.1"
                     /translation="ARLLAELVGFFRIEDDAPESEPPPPPRRESRAPARRAAPRAPQQ
                     AQRKEQPAKRNGRSERKNGANEEDGDWVEF"
BASE COUNT         1799 a         3540 c         3245 g         1685 t
ORIGIN      
        1 cgcgatgcgc cgccgatcgg gccgggaggc tctcactccg ttcgcgtgca tcatcggtgc
       61 ttgcctcgac gtagacggct cattggcgcc gggtcttaac acgagccggc ggagcgctgc
      121 acgcgcaggc tttgcgtcca cgaaatccgt tacttcgcaa attctcgaaa gattcgcgcc
      181 ggcacggacc ctgcttgcgg cctccgcatg cccgctagga tccgcaccat ggatggcaac
      241 gaggcagtcg cttcggtcgc ctatcgtctg aacgaagtca tcggcatcta cccgattacg
      301 ccagctaccc cgatgggtga gcagtgcgat gaatgggccg ctcacggccg tcaaaacgtc
      361 ttcggcagcg tccccactgt cgtcgagatg cagtccgaag gtggcgccgc aggcgccgtg
      421 cacggagcac tccaagccgg cgcgctgtcg accacgttta cggcttcgca gggcctgctc
      481 ttgatgatcc caaacatgta caaggtcgca ggcgagctcc tgccgtgcgt gatccacgtc
      541 gcggcccgga gcctggcaac gcacgcgctc agcatcttct gcgaccactc ggacgtgatg
      601 gcgtgccggc agacggggtt cgcccttctc gccgcgcgct cggcgcagca ggcgcacgac
      661 ttcgcgtgca tcgccacgtt ggcgacgcag gcagcccgcg tccccttctt gcacttcttc
      721 gacgggttcc gcacctcgca cgagctcgtg cgcgtagcag agctcgacga cgacgacctt
      781 cgctccctgc tcgacgaagg cttgctcgcg gagcgccggc gcaacgcgct cagccccgat
      841 cacccctccg tgcgcggtac ggcgcaaaac cccgacacct tctttcaggc ccgcgaagcc
      901 gcgaaccgct tctatgcggc gtgccccgaa cacgtcgaag cgacgatggc gcgcttcgcc
      961 gcgctgactg gacgcagcta tcgcctcttc gagtacagcg ggcaccccga ggcggagcga
     1021 gtggtggtgg ccatgggctc cgcgctagaa acgatcgccg gtacggtgga ggagctcgtg
     1081 cgccgaggcg aaaaggtcgg cgttctgggc gtcgcgctct ttcggccatt ctcggtcgag
     1141 cgcttcctcg cggcgctgcc cgttacggcc aagcgcattg ccgttttgga tcgcaccaag
     1201 gagcccggag ccatcgggga accgctctac ctcgacgtcg tcaccgcgct ctccgaggga
     1261 gaccgcagcg ccggcacccg cgtcatcggt ggacgctacg gcttgtcctc gaaagagctc
     1321 acgcccgcca tggtcaaggg cgtctacgac gagctcgcgc gcccgaaccc gaagcggcac
     1381 ttctcgctcg gtatccacga cgacgtcacg ttcgagtcgc tcgccttcga cgaagagttc
     1441 gagctcgacc gcggccagct gcgcgaggca ctgttcttcg gtctcggcgc cgacggcacg
     1501 gtcggggcga acaagaacac catcaagatc atcggtgatc atggccggtt cgaggtacag
     1561 ggcttcttcg tttacgactc gaagaagtcc ggagccgcga ctatttcgca cctgcgcttc
     1621 gatgagcagc ccattcgccg gccctacctc atcgagcgcg ccgatttcgt ggcctgccac
     1681 cagttcgaac ttctcgatcg gctgccggtg ttggatcacg ttcgcgacgg aggagtgctg
     1741 ctcatcaaca gcccgttccc tcccgacgcg acgttcaacc agctctcgcg cgaggtccag
     1801 tcggcgatca tcgacaagcg actgcgcgtg tatgtcatcg atggctatcg ggtcgcagtc
     1861 gacgcgggcc ttggaaagca catcaacacc gtgatgcagg cgtgcttctt cgccttgagc
     1921 gacctcatgc cgctcgagca gagcatcacc gccatcaaga aaggcatcga gtacagctac
     1981 gcgaagaagg gcgcggaggt cgtgcgccgc aatctcgaag ccgtagacgc cgcccgcacg
     2041 gcgctccgtg agctcactct ccccgctcgc gtcgacgaca gcgcgcgcgc ccggcgcccc
     2101 cccgtgccgg actccgcgcc cgacttcgtg cagcgcgtga cggccgcgat gatcgcgggg
     2161 ctcggggacc gcttgccggt gagcgcgttt ccaatcgacg gcacttggcc gagcgggacg
     2221 acccgcttcg aaaagcgaag gctcacgacg acggccccgg tctgggattc gaagctctgt
     2281 attcagtgca acaaatgcgt gctcgtctgc ccgcacgcgg ccatccgggc caaggtcttc
     2341 gagccgcaag cggctgcggg cgccccggag ggcttccaag cgacgccctg gaagggccac
     2401 ggcttccccg gcgagcaata ctcgctggcg gtcgctcccg aagattgcac cggctgcacg
     2461 ttgtgcgtcg ccgcgtgccc cgcgaaggac aagagcaacc ctcgccgcaa agcgctcgaa
     2521 atgcagacgc tcagcgacag cctcgatagc gagcgcaaaa cttacgactt cttcctgaac
     2581 ctcccgccgg tagaccgccg gcgcgtacgc ctcgacgtga aaggcgccgg cctcatcgac
     2641 ccgctcttcg agtactcggg ggcatgctct ggttgcggtg aaacgccgta cctcaagctg
     2701 ctgacgcagc tcttcggcga tcgcttgttg ctcgccaacg cgaccggctg ctcgtccatc
     2761 tacggcggta acctgccgac gactccctac gccaccgact ccgctggccg cggccccgct
     2821 tgggccaatt cgctcttcga agacaacgcg gagttcggcc tcgggattcg gcttggcgtc
     2881 gacgcccatc ggcggcacgc ccgcgagctc ctcgccgagc tccggacgcg gctcgacggc
     2941 gctctcgtgg atgccttgct cgccgcgcct cagggcgacg acaacgaaat ctacgcgcag
     3001 cgcgggcgag tgaacgagct ccgcaccgcg ctcgcgagca tcgacgatcc gcgagcgcgg
     3061 cgtctctcca cgctcgccga ctacctcgtg aagaagagcc tctggatcgt cggtggcgac
     3121 ggctgggcgt acgacatcgg cttcggcggg ctcgaccacg tgctggcctc gaacgaagac
     3181 gtgaacgttc tcgtgctcga caccgaggtg tactccaaca ccggcggtca gcgctcgaag
     3241 gctaccccac gcgctgcgtc ggccaaattc gcggcgctcg gcaaggacac cgaaaagaag
     3301 gatctcggct tgcacgcgat gagctacgga cacgtctacg tcgcgcgcgt ggccttcggc
     3361 gccaaggacg cgcagacgct gcgcgcgttc agcgaggccg aagcgcatcc aggaccctcg
     3421 ctcatcatcg cgtactcgca ctgcatcgct cacggttacg acatgaagtt cggcctcgac
     3481 caacagtcgc tcgccgtgcg ctccggggtg tggccactgt tccgcttcga tcccaagcgc
     3541 atcgccgagg gagaaccgcc gctcgccctc gacgcttcac ccggcaacgc gagcgtgacc
     3601 gaatacatgc gcggcgaggg tcgcttccaa ggcctcgagc gcctcgatcc cgcgcgctac
     3661 gaggagctga agagcgcggc cgagctggac gtgcgccggc gcatcgagct ctacgaagcc
     3721 ttgtctcaca ttcacttctc caccgcgaaa aaggattgaa cgatggacct ttcggctagc
     3781 tatctcggct tgaagctgcc tcaccctctc atcctcggcg cctcgccgct ggtcgacgac
     3841 ctggacacgg ttcgaagcgc cgaggacgcc ggcgccgccg ctatcgtcat gcactcactg
     3901 ttcgaggagc gcatcttgat ggaggcgggg gccgcgctgt cgctcgaaag ccacgaagac
     3961 accttcgccg aggccctcta tttccgcccg aaccgcgatc gcttcgcgct cgggccggac
     4021 gagtacctcg agaagatccg gcgcatcaag gacgtcgtct cgatcccgct gatcgcctcc
     4081 ctcaacggca ccaccgcgcg cggctggctc gagtacgcga ggctcatcga tcaggccggc
     4141 gcggacgcgc tggagctcaa cgtgtaccga gtcgcagcca acgcggacga aacggccgaa
     4201 ggcatcgaag ccgagaccgc ggacatggtg cgcgccatca agtcgaagac gaacctcgcc
     4261 gtcgccatca agctctcgcc gttctacacg tcgctctcca acgtcgtcga gcgcttgatc
     4321 gacgcgggcg ccgacggcct cgtcctcttc aaccgctttt accagcctga tttcgacttg
     4381 gaggcgctcg aggttcgtca cagcctcgag ctctcgagct cctccgagct cctcttgcgc
     4441 ctgcgctggc tagcgattct gtcggctcag ctcgcaccat cggttacgct ggccgcgagc
     4501 ggaggcgttc acaacgcggt agacgctttg aaggcagtca tggccggcgc acacggcgtg
     4561 cagatggtct cggagatttt gcaccacggc gtcaaccgtt tcgcagaggt gcgtcgtgac
     4621 atggaagagt ggctcgaaaa gcacgagtac gagtcgctcg cccaggcgcg aggcagcttg
     4681 aactcgacgc gctgtccgga cccgtctgcg tacgagcgcg ccaattacgt ccgcgtcctt
     4741 ggatcttggc gtggcaacgc gctccctgcc cgatgagcgc gcaagaactg cgtgccgcgc
     4801 gcggggtcgc gagaactgcg cgtcgaatct cacgccattc gcgcacgatg cttggattta
     4861 tcgcgctcga acgcggcacg ccgcttgctg aaacactagc gtcggtcacg agccgacatg
     4921 gaggagcctc gcaatgtcgc ccagtcccat caaacgaatt ctagtcgctg tagatctgtc
     4981 ggcttgctca cgcgcagcct tggagttcgc gcggcgcgtc gcagagccac tgggcgctag
     5041 cctcgaagcg ctctacgtgc tctcgcccga ccacggcgca gggcacgagc tcgagctcgc
     5101 cgctgcgcgc gaagagctgc atcgcttcgt ccattcggtc gtgggtgcca cccccgtcgt
     5161 cgagcacgtc gagcccggtg aaccgcgcga acggatcgtg gcggtttccg atcggggcgg
     5221 cttcgacgcc atcgtcatgg gtaccgaggg ccgaactggc cgcgcacgcg ctttcgctgg
     5281 cagcgtcgcg gagagcgtcg tgcgcacggc atccgtaccg gtcatgacgg tgcgagagaa
     5341 gcgtcactga tgagccgaat tgcgtgaccc gagcggcggt gtgcggcgac tcaccgctgc
     5401 tcgactcgcg cgccgtcctt gacgcggtcc cccgggtgca ccgcgacgag cgcgccttca
     5461 ccgattccgc ccgtgatttc agcctccgtt tcgccgcggt ggccaacgct caccggcgtc
     5521 aagcgagccg cgcctccttc gacgcggaac actgcccagc tctcgccatg acggaacagc
     5581 gccccttgcg gcacctttag cacgcgggag ccttgccaca gcacgatgcg cgtctcgact
     5641 cggtagccgt cgcccagcgc cgaccaactc tgttcggggc cggtcaacgc gacgacgacg
     5701 ttgacgcgct gttcatcgac ccccaacgcg gacggccgcg tgaacgcgga tggctctacg
     5761 cgacgcacgc ggcccgcgat gctgtggtcc ccaccccacc cccgaatcag caccgccgtc
     5821 ccaggcagca cgcgaacggc gtcggtcgtg agcaagtcga ccacgacctc gagatcgcca
     5881 gggttcccga cttcgacgag cggcgtcgcc gcctgcacga taccggcgct cttttggtga
     5941 acccgcaaga tccgcccgga tatcggcgag agtacgtcga tgtggcggtc tcgcgagccc
     6001 gcaccgtcgc ggccgagcgc cacccgcgcg acgcggacct cttcggtcgc gaccttgccc
     6061 gcgaactcgg cagaagatag ctcctctccg cgcatgcgag ccaggaactc gctttcgtcg
     6121 agtgcctgag ccgtgatcga gctcgaggcg aagagcctcc gcgagcgcga gagctcttgc
     6181 tcggcgagct ccttcgcgac ggacgctcgg gatatctgcg ctcgcgcctg gccgagcgcc
     6241 gagagcgcgg cgccgaggcg cgcttccgat tcggcgcggg tgcgctcgtc gagcaggggc
     6301 gaaacggccg gcgcaatttc cgccagcgtg tcgccctctt tgacgacgtc gcccgcttcg
     6361 aggcgcaggc gagacaagct ccccgtcacg ggcgcggaga ccacgaagcg atctttgacg
     6421 cgcgtcacac cgtcctcttc gatcaccacc gagaggtcac cgcgtttgac cggctcggcg
     6481 tcgaccggta ccggggccgg ccgcaacgcg acgaccgcgc cggcagcggc ggccacgatc
     6541 gcgagaccga gcaaggcgtg gcgtacgttc cgggcgagag cgcgcttgcg ttcacgcccc
     6601 ctcttcttcg cgggatcttg ggtcagccca ccgagagggc tcgtgaccga actcgtttcg
     6661 ttgctcgagg atgacatcac tctctcgctt tcaagacggc gattagatcg aggctatcga
     6721 gactacggcg cacccaaaga gcgctggcgg cggccgcgag cacggtcacg ccgatcgaca
     6781 agaggtaggt gctcggcgcg accaccaccg tccagcggaa aacttccgga tcgacggttc
     6841 gcatgaacgc gcgcgcccac acgctgccga gccacaagcc gattggaatg gcaatggcta
     6901 cttccagcac ctgactggag agcaagatcg tcgagatctc gcgccgagtg aacccgagca
     6961 cgcgcaggct cgcgagatcg cggctgcggg cggcgagccc gatacgcgcg ttgttgtaga
     7021 ccacgccgaa cacgatgctg gccgaaagga acaccgagac tgcggtccaa acgttcatga
     7081 tcgaagcgtt catgtcgagg gtccgctgca tgtcaccttt gacgtcggag atgtcgatga
     7141 cgtgcggaga ctcgcgcaag cgcgcgttca ccgtgtcgac gaacgggcgg tcgattttca
     7201 gcagcaccgc cgagaccgct cccgtgtcgc cctccaggct cgcgagctcg tcggtctgag
     7261 cgtaaatcga caagccgatc gactcgtcta cgaacccgag gacgactggg cgaacactgc
     7321 ggcggtcgcc ttctcgaagc tcgatctgcg ggcggtcgcc gacgtgaaga cccagcacgt
     7381 cgccgagcgt cttggtgagc acgattccgt caggcaagac cggaacctcg gcgcgctttc
     7441 ggtcgaccag acgccgcagc gtgctcgctt cgggcaatcc catgagcacg gattcgcgag
     7501 aacggtgctc gaaggttacg cgaacgggca ccgcgcgaat cccttcggcg cgaatcaccc
     7561 ccggcaagcg tccgagctcc gcgacgacgc ggggcgacac gggacgcgcg aacaccaccg
     7621 acacgtcttg gcgctgctcg cgcaagaagg tgccctcgaa gtaggcgagg atcgaatcga
     7681 ggccgaagtg actgaatatc aagagcgaga ccgcccctgc gataccgatg ctcgagagca
     7741 gcgtgcgcag cggccgacga gcgacctcgc gaaccaccat cgtaccgctc gggccaatga
     7801 gcccgccgag cccgagccgt tcgagccagg tccgcttgta agtcgcggga gccggcggct
     7861 gcatcgcctc cgcggggggc atgcgaaccg cggagcgcac cgcgccgagc gcgcccacga
     7921 gcgccgcgat cgcgctcacg aggagcgccg acgccatgag cgcaaacgtc atgcgaaagc
     7981 gaagctcggg aaagcgaaac gacgtcgcgt agagcccgag cacgacgcgc ccgaggccga
     8041 gcccacccaa aagaccgagc acgccgccgg gaaccaagac aatcgcgacg agcccaaggt
     8101 aatgccgccc gatctcccag ttcgtgtatc cgaccgcctt cagcgttgcc acttcgggtc
     8161 gctgcagccg aatcagccga cccagcacca agttcaccag gaacgcggcg acggccagga
     8221 acaccgcggg caccatcgtc gagagcgctc cgagctggtc gagctcctga ctcacgatct
     8281 tgttcgagat ctggtctttt cggccgatcg agcccgttcc tccgaacggc ccgagaacgc
     8341 gatccaccgc ggtccgcacc gcttgctccg atgcgttggg cgaaatccgc agcgagacat
     8401 cgttgaatgc tcccccgagc tggaacgcgc tggcgagcgc cgttcgctcc atccacagca
     8461 ccgcgtgccg cttcgggtcg tcgaccatcg ccccagggcg aatcgcgtac acgaactcgg
     8521 gtgacaacgc gatgccgact actcgcagtt cgcggcgctt accgttcatc acgaccggca
     8581 cacgttgccc ggggtcgata ccgtgcgctt ccgcgaacga ctcgagcagc acgacctcgt
     8641 cctgatgcgt cgcttccggc agccgaccct tgcgcaagaa caacgcgttc gtggccggag
     8701 tgcgtccggc tgggagcgac agcagtcgac cataagcggg acgcggcatg ccctctatcg
     8761 gcacggtcac gtcttcgctc acccgcgttt gaaggacttc gacccccggg atctcctcga
     8821 ggcggcgagc cgtcgtctcg gggactcgct ccgcttgagc aaagacatgc gcgaagcgat
     8881 accggtcgta gtaaacgagg cgtgactcgg cgatcgaaat gtacgtccct cgaagcgcga
     8941 tgaagcagat gatgccgctc gccaaaacca gagcgatcgt cgcgatctgc cctttcaacc
     9001 gcgtcgtttc gcggagtagt ttgcgatcga gcgctcgcgt gaacatggct tcaccagcga
     9061 agcgcgcgcg ccgccgctgg ctcggggttc gaggtttcgc tctcgacgag cccgtctctc
     9121 agcgtgacga cgcgatcggc catggccgcg atgggcgcgt tgtgggtgat gatggcggtt
     9181 gccgtgccga gttcgtggtt gacgcgctcg agcacctcga gcacgcgcac cccggtccga
     9241 aagtcgaggg cgcccgtggg ttcgtcacac agaaggacgg acggtcgctt ggcaaccgcg
     9301 cgcgcgatcg caacgcgctg ctgctcaccg ccggaaagct gcgtcggaaa gtggtcgagg
     9361 cgctcaccga gcccaacgag cgccagggcg tcctcggctt cgagcggatc gtcggcgatg
     9421 tcggtcacga gcgcgacgtt ctcgcgcgcg gtgaggctcg gaatcaggtt gtagaattga
     9481 aatacgaatc caacctggtg acgacgatat gcggtgagct gatcgtcgtc ggcctcggtc
     9541 aggtcttcgg tgccgaagaa gacgctcccc gacgtcggga cgtcgaggcc acccaagata
     9601 ttgagaaggg tcgactttcc gctgcccgaa gggcccaaaa gcaccaataa ttcgccgcgg
     9661 cgaagctcga aatcgactcc gtcgagatcg cgcacgtcga cctcgcccat ccggtaaatt
     9721 ttccggagcg cgcgacacga gagcacgctg ttcggcgctt cattcgcaga agtcgtatgc
     9781 gccggcgcgg ccatgaccat gtaggcagca aggcccgtac cagcgcgtct ctcgagcttt
     9841 gtcctgacat tcgagagaat tctccatctt ctttttgcgt cgatgacgac tttcgcgcag
     9901 accctgcgca gtctcccgcg aggtgcaacg gctcgtgagc ggaagctaca tcgcggcgac
     9961 gacacgactg aggccgagct gaaaacgaaa cgaggggctc cttgcgtgtg ccaggagtcc
    10021 ctcgtggttt cagccgaacg gtctcagaat tcgacccagt ctccgtcctc ttcgttcgcg
    10081 ccgttctttc gctcggagcg cccgttacgt ttcgcgggct gctccttgcg ctgtgcttgc
    10141 tgaggagcgc gcggagctgc cctgcgcgcg ggggcacgag actctcggcg tggaggcggg
    10201 ggcggctccg actcgggggc atcgtcttcg atacggaaga acccgaccaa ctcggcgagc
    10261 aaccgcgcc
//
LOCUS       DASTKS010001101         7423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1290094, whole genome shotgun sequence.
ACCESSION   DASTKS010001101 DASTKS010000000
VERSION     DASTKS010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290094"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..641
                     /gene="purE"
                     /locus_tag="VFQ35_23225"
     CDS             135..641
                     /gene="purE"
                     /locus_tag="VFQ35_23225"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9933644.1"
                     /translation="MSPSPSNRPLVGVIMGSQSDWETMRHAVEHLDLLGVPHEVQVVS
                     AHRTPDLMFDYAGSAESRGLEVIIAGAGGAAHLPGMVAAKTVLPVLGVPVQSHSLNGL
                     DSLLSIVQMPAGVPVATFAIGKAGATNAGLFAASLLGAKHPEIRAALSRFRAERTQSV
                     LDAPDPRK"
     gene            610..1749
                     /locus_tag="VFQ35_23230"
     CDS             610..1749
                     /locus_tag="VFQ35_23230"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET9933645.1"
                     /translation="MSSTHLIPESSAPFEARSVGVLGGGQLGRMLALAGYPLGVHCKF
                     VDPTPNSPAGAVAPQIAADYVAREAIRELAACEVVTYEFESVPVEAAHELAETTPVFP
                     PPNALATAQDRLSEKECFRALGVPTAPFAPVSTEAELRDAVARLGLPAVLKTRRLGYD
                     GKGQCVLRSMSDVEPAFRSLGGVPLLLEGFVKFERELSLLAVRGRDGSIAFYPLVENH
                     HRDGILRVTWAPAPNLDAALQNEAEEHVRKVLERLDYVGVLAVEFFACEGRLFANEMA
                     PRVHNSGHFSIEGARTSQFENHLRAVLGLPLGSTEVPEPCAMLNLVGELPTREAVLAV
                     PGAHLHLYDKTPRTGRKVGHITVRAADRTELTRRVEALLRLPGAG"
     gene            complement(1860..3278)
                     /locus_tag="VFQ35_23235"
     CDS             complement(1860..3278)
                     /locus_tag="VFQ35_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933646.1"
                     /translation="MVLTALGYLFAPRADPYITARGFSLAAIAFIAFAVYRLVRRASK
                     GALPALLAVALTLSCTDLFRAAISGLETTAFSAAIVGGWYAYLREPAERRKWSLFAFL
                     PAALLRIDGFVPLLVALGFEVLVAFQERRFSVRAIARWALPALGIWALYFAWRYFYYG
                     LPLPATYYAKTMVTANEPQRGVHQLWAFFHDYGLIAFLPVAFVPLFWGPRREACALWT
                     AIVLQLGYAAHVGGDWMPFQRFFVPVVPLGGVLAGYGLDRTWSALRALRPLLAWPLRG
                     AAVAVLGFTAVHMYIGSLDTPEEREKLGGAQHSERHTRDNLIRNADLMRYVIRRPGER
                     LVTDYAGVFSVMTDAAIIDMWGLCTADIALHGNIEGINPIYGKACAECYARLDPEYFH
                     VMVPIVRSRSAFRNQREVIDAVFQGWAIDRYIGIQQNFVVGRVYENATGRTLWFLEKR
                     RAGLSYAARSPANGLVIDYPFG"
     gene            complement(3306..3527)
                     /locus_tag="VFQ35_23240"
     CDS             complement(3306..3527)
                     /locus_tag="VFQ35_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933647.1"
                     /translation="MVSAAFSRAWLSVRELLRTPRGILWLLAWIPIVALCVIQLELVG
                     DFRIDDAYITFSFSKNLAAGNGPVFSHGV"
     gene            complement(3641..4219)
                     /locus_tag="VFQ35_23245"
     CDS             complement(3641..4219)
                     /locus_tag="VFQ35_23245"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9933648.1"
                     /translation="MLVSRRSLLFGALALAACRSKPPLPDLGKVPSFSLQSEAGTPFG
                     SQNLSGRVWAAAFMFTRCPTVCPRVTRFMQKVQASSKAAGVELMLISISVDPENDSPA
                     VLKEYAERFHADLSSWKFLTGDFELIKRTSEEGFKLALEGRADPKAQDFGIVHGSHLV
                     LVDRSSNIRGYYRTEDEAEATRLLADAKRLAS"
     gene            4621..5331
                     /locus_tag="VFQ35_23250"
     CDS             4621..5331
                     /locus_tag="VFQ35_23250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9933649.1"
                     /translation="MADAPQPNQKFDGYARDYDRLLEDSIRASGESKEYFLQYKLDCL
                     ERLGVNFDAPLLDYGCGVGNLSHKLAERFARVEGYDPSPESLAVARRQSSRVTFHHET
                     STLEDGKFSTAVLSGVLHHVPPPEREAVMSTVRQKLAPGGKVVVFEHNPWNPVTRRAV
                     ADCEFDDDAILLWPGELRGLLRASGYADVRLEYIVFFPRPLAALRPLEPRLAWLFMGA
                     QTMTIGTQPSAGTHSART"
     gene            5408..6505
                     /locus_tag="VFQ35_23255"
     CDS             5408..6505
                     /locus_tag="VFQ35_23255"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9933650.1"
                     /translation="MNAEAEDAAPPAPEPEKTSAEQAALPARPEASEAKAKVASSPRG
                     GVVEWFWGRRAMKAARAHGSGPSAVGLAYQREANLCIELARNALEPLQPLEFGPAQAS
                     ACELYRQAIYWALLAESTATAKPEGKNSFDEVWDGSDRALLLKAAGGNAAQLDSIGQA
                     LKGKGFADFADLSPAAARELSTTLRQFAEALVDALDPAQLEVERIWLRRMTRVGTLLG
                     LAVFALVTSVFVADARERARDVARGKPWEISTNYGEGCASPDQTCENSPQFFFHTLEQ
                     NDPWFIVDLQEPTSISGLRVDNRGECCLERAIPLVVEVSTDRQNWSEVARRTTEFGTW
                     RVSFAPVQARYVRLHVPRVTNLHLKRVRVLR"
     gene            6678..>7423
                     /locus_tag="VFQ35_23260"
     CDS             6678..>7423
                     /locus_tag="VFQ35_23260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9933651.1"
                     /translation="MAVSPPQAEETSDTRREDALVRWAGTESAPLVVLCGFLFLLGAY
                     LRLKNFGYPQSFQFDEHHFVENARNYLNHRADWNDHPPLGKLFIAASIAALGDNAFAW
                     RLPSLIFGACTVALAAVVARRLFNSAWAGLFAAAFASVDGFLVAYSRSALLDGALCAM
                     GLLSLLAATLERKRLLLWGSIAAGVAMSVKFSGVGALVAPSVALLCSSSKRSAKVSAL
                     VSLGIGALAIYVVCYWIGFRLQGMPLGVQPV"
BASE COUNT         1336 a         2433 c         2404 g         1250 t
ORIGIN      
        1 gacggaaagc cggtgctcga cgacgaaacc tacgagcggg tcctcgcgta tcgtcgcgaa
       61 tacggtggca aaatcgcctg acgtaccgtt ttgcttgccc gaacccgaac gagcgataaa
      121 gtccgcctcc ttccgtgtcc ccctccccct ccaatcggcc gctcgtcggc gtcatcatgg
      181 gctcccaaag tgactgggag accatgcgtc acgcggtaga gcacctggat ctgctcggcg
      241 ttccccacga agtgcaggtg gtttcggcgc atcgcactcc cgatttgatg ttcgactacg
      301 cgggcagcgc cgagagtcgt gggctcgagg tgatcatcgc tggcgcgggc ggcgcggcgc
      361 accttcccgg catggtcgcg gcaaagacgg tgctgccggt gctgggcgtc ccagtccaga
      421 gccacagcct caacgggctc gactcgctct tgtcgatcgt gcagatgcca gcgggagtgc
      481 ccgtcgccac gttcgcgatc ggtaaggcgg gagcaactaa cgcaggtctc ttcgcggcga
      541 gccttctcgg cgccaaacac cccgagatcc gcgccgcgct gagtcggttt cgggcggagc
      601 gcacgcaaag tgtcctcgac gcacctgatc ccagaaagta gcgccccgtt cgaggcgcga
      661 agcgtcggcg tgctgggcgg cggccagctc ggtcgcatgc tggcgctcgc cggttatccg
      721 ctcggcgtgc attgcaagtt cgtggatccg acgccgaatt caccggcggg agccgtcgcg
      781 cctcaaattg cagccgacta cgtcgctcgc gaagcgatcc gcgagctcgc cgcctgcgag
      841 gtggtgacct acgagttcga gagcgttccc gtcgaggccg cccacgagct tgccgagacg
      901 accccggttt ttccgccgcc gaacgctttg gccacggccc aggatcggct gagcgagaag
      961 gagtgctttc gtgcgctcgg ggtcccgacc gcgcccttcg ccccggtttc gaccgaagcc
     1021 gagctgcgag atgcggttgc gcggctcggg ctaccggcgg ttttgaagac gcgccgcctc
     1081 ggctacgacg gcaagggtca gtgcgtgctg cgaagcatga gcgacgtcga acccgctttc
     1141 cgttcactcg ggggtgtgcc gctcctgctc gaaggcttcg tgaagttcga gcgtgagctc
     1201 agcctgctcg ccgtgcgcgg gcgcgatggg agcatcgcct tctacccctt ggtcgagaat
     1261 catcatcgcg acggtattct gcgggtcacc tgggctccgg cgccgaacct cgacgccgcg
     1321 ctgcagaacg aagcggagga gcacgttcgc aaggtgctcg agcggctcga ctacgtcggt
     1381 gtgctggcgg tcgagttctt cgcctgtgag ggccggctgt tcgcgaacga gatggctccc
     1441 cgtgtccaca actcggggca cttctcgatc gagggagcgc gcacgagtca attcgagaac
     1501 cacctgcgag ccgtgctcgg gctgccactc ggctcgaccg aggtgccaga accgtgcgcg
     1561 atgttgaact tggtcggcga gctaccgacg cgtgaagccg tgctcgcggt acccggcgcg
     1621 cacctgcacc tttacgacaa gacgccgcgc acgggccgca aggtcgggca catcaccgtg
     1681 cgtgcggcgg atcgtaccga gctcacgcgt cgagtcgagg cgctgcttcg tctgcccggc
     1741 gcgggctaac gtcccctcgc cctcggtgaa cctcgagttg cttcgtttgc ccggcgcggg
     1801 ctgacgtccc cgctcccctc ggtgaactcg gcgacagcgc gtgaacgcgt gttctcgcct
     1861 cagccgaacg ggtaatcgat cacgaggccg ttcgcaggcg atcgcgccgc gtagctcaaa
     1921 ccggctcgcc tcttctcgag aaaccagagg gtgcgcccag tcgcgttctc gtagacgcgc
     1981 ccgaccacga aattctgttg gatgccgatg tatcgatcga tcgcccaccc ctgaaacacg
     2041 gcgtcgatca cctcgcgctg atttcggaat gcgctccgag accgcacgat cgggaccatg
     2101 acgtgaaagt attcgggatc gagacgcgcg tagcactcag cgcaggcttt gccgtaaatt
     2161 gggttgatgc cctcgatgtt gccgtgcaac gcgatgtcgg cggtgcagag gccccacata
     2221 tcgatgatgg ccgcgtcggt catcaccgag aacacgccgg catagtcggt gacgagccgc
     2281 tcgccggggc ggcgtatcac gtagcgcatg agatccgcgt tgcggattag gttgtcgcgc
     2341 gtgtgtcgct ccgagtgctg cgcgccgccg agcttttcgc gctcctccgg cgtgtcgagc
     2401 gagccgatgt acatgtgaac ggcagtgaag ccgaggacag ccacggcagc gccccgcaac
     2461 ggccacgcca agagcggcct gagcgcacgg agcgcgctcc aggtgcgatc gagcccgtag
     2521 ccagcgagaa cccctccgag cgggacgacc ggcacgaaga agcgctgaaa aggcatccag
     2581 tcgcccccga cgtgagccgc gtacccgagc tgaaggacga tcgcggtcca gagcgcgcaa
     2641 gcctcccgcc gcgggcccca aaacagcggg acgaacgcga ctggcaagaa cgcgatcaag
     2701 ccatagtcgt gaaagaaggc ccagagctgg tgaacgcccc gctgcggctc gttcgcggtg
     2761 accatggtct tcgcgtagta ggtcgccggc agcggcagtc cgtaatagaa gtaacgccac
     2821 gcgaagtaga gcgcccaaat cccgagcgca ggcagcgccc agcgcgcgat cgctcgcacc
     2881 gagaagcggc gctcttgaaa ggccacgagc acctcgaagc cgagcgcgac gaggagcgga
     2941 acgaaaccat cgatgcgaag cagcgcggcc ggcaagaacg cgaacagcga ccactttcgg
     3001 cgctcggcag gctcgcgcaa gtaggcatac cagccgccca cgatagcggc gctgaatgcg
     3061 gtagtttcta aacccgaaat cgcagcccgg aacagatccg tgcaagagag cgtgagcgcg
     3121 accgcgagca gcgctgggag agctcccttc gaagcacgcc gaaccaagcg gtaaacggcg
     3181 aaagcgatga aggcgatggc ggcgagcgag aagccccggg cggtgatgta cggatcggcg
     3241 cgcggcgcga acaaataccc gagcgcggtg agcaccatcc aaaggaagtt cgaatagcct
     3301 tcgactcaca ccccgtggct gaagacgggc ccgttaccgg cggcgaggtt cttggagaac
     3361 gaaaaggtga tgtacgcatc gtcgatgcga aagtcgccga cgagctcgag ctgaatcacg
     3421 cacagcgcta cgatcgggat ccaggcgagg agccagagta tcccgcgcgg agtccgtaag
     3481 agctcgcgaa ccgagagcca cgcccgcgaa aaagccgcag aaaccatccg ggcgcgcagc
     3541 atagcagcaa ctctcgaagc gccgctcgcc gaatcaaggg gttcgccgcg ttcgttacgc
     3601 gcgcggaggc cgtacgcgcc cgggtccagc gggccgcgac tcagctcgcg aggcgctttg
     3661 cgtcggctaa caagcgggtc gcttcggcct cgtcttcggt gcggtagtag ccgcgaatgt
     3721 tggagctccg gtcgacgagc accaagtgcg agccgtgcac gattccgaag tcctgagcct
     3781 tcgggtccgc ccgcccctcg agagcgagct tgaagccctc ctcggaggtg cgtttgatca
     3841 actcgaagtc accggtcaaa aacttccagc tcgacagatc cgcgtgaaac cgctctgcgt
     3901 actccttcag caccgcgggg ctgtcgttct ccggatcgac actgatggag atcagcatga
     3961 gctccacgcc cgccgccttg gaagacgcct gaaccttttg catgaagcgc gtgacgcgcg
     4021 ggcacacggt cgggcagcgc gtgaacatga acgcagccgc ccaaacccgc cccgagaggt
     4081 tctgcgagcc aaacggcgtc cccgcctcgc tctgcagaga gaacgaaggc accttgccca
     4141 aatcggggag cggcggcttc gagcggcagg ccgcgagcgc gagcgccccg aagagcaacg
     4201 accggcgaga aacaagcatc gtcgggcctc gccatagcgc aaaggctcgc atttgcacgc
     4261 caaggcaggc cctgcaccga aaccttgcct tccggatcca agtgctgtag ggagctatcc
     4321 cacacgcgcc aatctggagg tttgccatgc gtcgaattcg aaagctcctc gagaagctgc
     4381 gcctgccgct gcccgcattc ggctgagcga ctcccggtga atcgctgatt caccgtgctt
     4441 ttgagccggg acgggcgagc ggcacgctct cgagcccggt attttcgagg gcctcgtcca
     4501 aatcggggcc ctcgaaaagt gtccggcgtc gctcgagaga gcagacgcgc agttcgagtt
     4561 cgcctgcggc ggcgcgctcg gcgctgctag atgcgcgcac ggcgctatag tcggcgcctc
     4621 atggcggacg ccccgcaacc gaaccagaaa ttcgacggtt acgcacgaga ctacgaccgc
     4681 ttgctcgaag acagcattcg cgcgagcggg gagtcgaagg agtactttct ccagtacaag
     4741 ctcgactgcc tcgagcggct cggagtgaac ttcgacgctc cgttgctcga ctacggttgt
     4801 ggcgtaggga atttgtcaca caagctcgcc gagcgcttcg ctcgagtcga aggctacgac
     4861 ccgtcccccg agagcctggc ggtcgcgcga aggcagtcat cgcgcgtcac gtttcatcac
     4921 gaaacgagca ccctcgaaga cggaaagttc tcgaccgccg tgctctcggg agtgctgcat
     4981 catgtgcctc cgccagagcg agaagcggtc atgagcaccg ttcgccaaaa gctcgctcca
     5041 ggcggcaaag tagtcgtatt cgagcacaat ccgtggaacc cggtgacccg ccgggccgta
     5101 gctgattgcg agttcgacga cgacgcgatc ctcctctggc cgggtgagct ccgtgggctc
     5161 ctgcgcgcca gcggctacgc cgacgtgcgc ctcgaataca tcgttttctt cccgcgccct
     5221 ctcgcggcgc tccggccgct cgagccgcgc ctcgcgtggc tcttcatggg cgcgcagacg
     5281 atgaccatcg gcacccagcc gagcgctgga actcactctg cccgaacctg aaaaatcccg
     5341 ctcgacggtc gagatcaaga gggttagcct cgggccgtcc tggggattgt cgaaagggaa
     5401 tagtttcgtg aacgccgaag ccgaagacgc ggcaccgccc gcgccagagc ccgagaaaac
     5461 gagtgccgaa caagccgcgc ttcccgcacg gcccgaagcg tccgaggcga aggctaaagt
     5521 cgcgagctcg ccgcggggcg gtgtcgtaga atggttctgg gggcggcgcg ccatgaaggc
     5581 ggcgcgagcg cacgggtcgg gcccgagcgc ggtcggcctt gcctatcagc gcgaagcgaa
     5641 cctgtgcatc gagcttgctc gcaacgcgct cgagccgctg caaccgctcg agttcgggcc
     5701 ggcgcaggcg tccgcctgtg agctctaccg ccaagcaatc tattgggctt tgttggccga
     5761 gagcaccgct accgccaagc ccgagggaaa aaacagcttc gacgaagtct gggacggcag
     5821 cgatcgcgcg ctgctgctca aagccgccgg cggcaacgcc gcgcaactcg acagcattgg
     5881 ccaagctttg aagggtaagg gtttcgcgga tttcgcggac ctgtcgcccg ccgccgcgcg
     5941 cgagctcagc acgacgctcc ggcaattcgc cgaggcgctg gtcgacgcgc tcgaccccgc
     6001 gcagctcgag gtcgaacgca tttggctgcg ccgcatgacg cgagtgggca cgctgctcgg
     6061 cctcgccgtc ttcgccttgg taacgagcgt cttcgttgcc gacgcccgcg agcgcgcacg
     6121 cgacgtcgcc cgaggcaagc cgtgggaaat cagcaccaat tatggggagg gctgcgcctc
     6181 gcccgatcag acctgcgaaa atagcccgca gttcttcttt cacaccctgg agcagaacga
     6241 tccgtggttc atcgtcgacc tgcaggagcc cacttcgatt agcggccttc gcgtcgacaa
     6301 ccggggtgag tgctgtctcg agcgcgcaat cccgctcgtc gtcgaagtca gcaccgaccg
     6361 ccaaaattgg agcgaggtcg cgcgccgaac caccgagttt ggaacgtgga gggtttcatt
     6421 cgcccccgtg caagcgcgct acgtgaggct ccacgtcccg cgcgtcacga acctgcattt
     6481 gaagcgcgtg cgcgtgctgc gctaggcggc gtcgatttac gggctctgcc gctcgaggtc
     6541 agtcgtccct ggggctcgct cgtcctcggc ttgcccgcgc cgcgtgaaag ccagttcccg
     6601 cggagaaggc gactcgagtc actgcgatcc gaaatactct atgaaaaatc gcgtctgccc
     6661 cttagggttg gcgcggaatg gctgtgtcgc ccccccaagc cgaagagacg tccgacactc
     6721 ggcgtgaaga cgctctcgtc aggtgggcgg gaacggaatc ggcgccgcta gtcgttctgt
     6781 gcggcttcct cttcttgctc ggcgcgtact tgcgcctgaa gaactttggg tatccccaga
     6841 gctttcaatt cgacgagcat cacttcgtcg agaacgcacg taactacctg aaccaccgcg
     6901 cggactggaa cgaccacccg ccgctcggaa agctcttcat cgccgcatcg atagcggctc
     6961 tcggcgacaa cgcctttgcg tggcggcttc cctcgttgat ttttggagct tgcacggtgg
     7021 cgctggcggc ggtcgtcgcg cgccggctct tcaactctgc gtgggcgggc ctatttgcgg
     7081 ccgcgtttgc gtcggtagat ggcttcctcg ttgcttattc tagatcggct ctgctcgacg
     7141 gcgcgctctg cgccatgggc ctgctctcgc tgctcgcggc gacgctcgag cgaaagcgct
     7201 tgctcctctg ggggagcatc gccgctggcg tggccatgtc cgtgaagttc tctggagtcg
     7261 gcgcgctcgt tgccccctcg gtggcgctgc tctgttcgag ttcgaaacgc tctgcgaaag
     7321 tctcggcact cgtcagcttg ggcattggtg ccctggccat ctacgtcgtc tgctattgga
     7381 tcgggtttcg tctccaaggg atgccactcg gggttcagcc ggt
//
LOCUS       DASTKS010001102         4043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1960888, whole genome shotgun sequence.
ACCESSION   DASTKS010001102 DASTKS010000000
VERSION     DASTKS010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960888"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFQ35_23265"
     CDS             <1..304
                     /locus_tag="VFQ35_23265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933652.1"
                     /translation="KALLIDDDASGHDFAFKLGECGYYTADKNLYSNAVDRLTQSSLA
                     KLEARAKGTEKPQPAEPAPLLTDAEKEHLQGLIGLTAMDSIYDYFRGARTEESGTV"
     gene            409..1074
                     /locus_tag="VFQ35_23270"
     CDS             409..1074
                     /locus_tag="VFQ35_23270"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET9933653.1"
                     /translation="MVTLPMFDGLLPDDVKTAWGDPREDAPIALPAEEALIERAVEKR
                     KREFRKARACARSLLHEFGVREFPLLSGPQREPLWPEGLIGSVTHTDGLCAVAVASRR
                     LYAGIGIDVEPAEPLSERIANRVCSAEELAIARAQNELDALTLARVLFSAKEAFYKAQ
                     FCVTQKFLGFSDVAVELGGNGVFFVRVVADVAELANTQFVGRWASRGGFVLTSSWLGQ
                     KLG"
     gene            1081..2712
                     /locus_tag="VFQ35_23275"
     CDS             1081..2712
                     /locus_tag="VFQ35_23275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933654.1"
                     /translation="MASQSQPEVLSTGDFDFIESSWGYETAPADSTPDELLGVTLNGT
                     YAVERVLGEGGMGRVYLARHTRVAKKRVAVKVLREEYARNEEVLQRFQREAEAGAAVS
                     HPNVMTVFDVDRTPNGAAYLVCEYLEGVDLGAHLKQHNRVTVRAALHIAEALCHGLIA
                     AHASGVIHRDLKPQNVFLLGDRSRGAAACPDVKILDFGLSRFLDAGDGSLTKTGYIMG
                     TPAYMAPEQARGQRVDQRVDVYGVGAILYTALTGRPPFDEDTPQATVLAVVSSEPPRP
                     RSLVPSIPVAVELLLERALAREPADRYPDMTALLRAVEAVVADSPVTTPEVVQVPNLS
                     RVAPSLADVENETKAARPRLVLYLAAAAFLLVGGIITAVTGIELVKGYGFNRTELRLL
                     LLAILGISLTPATLWLLRIRSHVWDKSSRVLELLGHLRAAVLSFTVSYGVLALLMRFF
                     DDFLVRLIGHTKVQPIGASWPGWSLVLAATGLMLALSTLVRRAMDGAVRSRVGRAFVR
                     TLVLGITIVGFLGLLYFGWRWHALYVHPPGAHSPM"
     gene            2877..3248
                     /locus_tag="VFQ35_23280"
     CDS             2877..3248
                     /locus_tag="VFQ35_23280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933655.1"
                     /translation="MKPRKVLIVDDSETIRRQVAEALAPAGFEVVEAADGVEGLERAA
                     QHELSLMILDVNMPRLSGLEMLEKLKRDPRGAKIPVLMLTTEVQQSMVDRAKKSGAIG
                     WMIKPVKLDLLVSTVNKAVGT"
     gene            3264..3779
                     /locus_tag="VFQ35_23285"
     CDS             3264..3779
                     /locus_tag="VFQ35_23285"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9933656.1"
                     /translation="MLSENDAVSARVDTLTTRACEELFASYGVALTPVRGGFEDANES
                     VLSGVMGFIGTRLRGTCLLASVLGPLEASRPAGNNSRDWIGELTNQLVGRLKTKLLAR
                     GVEVFLTTPIVLRGACVQPISRGSREPVWFSSAFGKLMVWVEVECGRDLMLASEIPLS
                     TAGDAGDILMF"
     gene            complement(3783..>4043)
                     /locus_tag="VFQ35_23290"
     CDS             complement(3783..>4043)
                     /locus_tag="VFQ35_23290"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9933657.1"
                     /translation="IVVQTGVLSQRLEQVDLYRVNDYAVERPFAQRLVGTGNLFLKTA
                     DKSSPEIALRGIKTDVVALYERVRAATELDKSRRGVRLVDNE"
BASE COUNT          672 a         1307 c         1316 g          748 t
ORIGIN      
        1 caaggcactg ctgatcgacg acgacgcctc tggccacgac ttcgcgttca agctcggcga
       61 gtgcggctac tacacggcag acaagaacct gtactcgaac gcggtcgatc gcctgaccca
      121 gagcagcttg gccaagctcg aagcgcgcgc caagggcacc gaaaagccgc aaccagcgga
      181 gcccgcgccg ctcctcaccg acgcggaaaa ggagcacctg caagggctga ttgggctcac
      241 cgcgatggac tcgatctacg actactttcg cggcgcgcgc acggaagaat cgggcacggt
      301 ttgattccgg ccgcggggcg cgcctcgctc gcgagtccgg cctccgcgag aaagcgctga
      361 aaccctcggc tcggagtgcc cgccttttcg ccatggccgc gcagtgccat ggtgacgctc
      421 ccgatgttcg atggcctcct gccagatgac gtgaagacgg cgtggggcga tccccgcgag
      481 gacgccccca tcgccttgcc cgcagaagaa gcgctcatcg agcgcgcggt ggagaaacgg
      541 aaacgagagt ttcgaaaagc gcgcgcgtgc gctcgttcgc tgttgcacga gttcggtgtt
      601 cgcgagttcc ctctcttgtc ggggccgcag cgtgagcccc tctggccgga agggttgatt
      661 ggcagcgtta cccacaccga cggcctgtgt gcggtcgcag tggccagccg ccgactgtac
      721 gccggtatcg gcatcgacgt agagccggcg gagccgctct cggagcgaat cgcgaaccgt
      781 gtgtgttcgg cagaagagct cgcgatcgcc cgcgcacaga acgagctcga cgcgctcacg
      841 ttggcgcgag tgctattttc agccaaagaa gccttttaca aggcgcagtt ctgtgtgacc
      901 cagaagtttc tcgggtttag cgacgtggcg gtggagctcg ggggtaacgg cgtcttcttc
      961 gtacgcgtcg tcgccgacgt cgcggagctc gccaatacgc agttcgtcgg ccgatgggct
     1021 agccgaggtg gctttgtgtt gacctcgagc tggcttggtc aaaagctagg ctgagcccag
     1081 atggcgtcgc agagccaacc cgaggtgctg tcgaccggcg acttcgactt catcgagagc
     1141 tcgtggggat acgagaccgc gcccgccgac tcgacgcctg acgagctgct cggcgtgacg
     1201 ctcaacggaa cctacgcggt cgagcgcgtg ctcggcgaag gcgggatggg tcgcgtttac
     1261 ctcgcgcgcc atactcgcgt cgccaaaaag cgcgtcgcgg tgaaggtgct gcgcgaagag
     1321 tacgcccgaa acgaggaggt gctgcagcgt ttccagcgcg aagcggaggc tggcgccgcc
     1381 gtctcccacc cgaacgtgat gacggtgttc gacgtcgacc gcaccccgaa cggcgccgca
     1441 tacctcgtct gcgagtacct cgaaggggtc gacctcggag cccacctcaa gcagcacaat
     1501 cgagtcaccg tgcgcgcggc gttgcacatc gcagaagcgc tctgtcacgg gctcattgcc
     1561 gcgcacgcga gcggcgtgat tcaccgtgat ttgaagccgc aaaacgtctt tttgctgggc
     1621 gataggtcgc gcggagccgc agcgtgcccc gacgtgaaga tcttggactt cgggctgtca
     1681 cgcttcctcg acgcgggcga cggcagcctc acgaagacgg gctacatcat gggcactccg
     1741 gcgtacatgg cgcccgagca ggctcgcgga cagcgcgtcg atcagcgcgt cgacgtatac
     1801 ggagtcggcg cgattctcta caccgccctc acgggcaggc cgcccttcga cgaggacacg
     1861 ccccaagcga cggtgctcgc cgtggtcagc agcgagcccc cgcgcccgcg ctctttggta
     1921 ccgagcatcc ccgttgccgt cgaattgctg ctcgaacgcg cactggcacg cgaacccgca
     1981 gatcgctacc ccgacatgac cgcactgctg cgagccgtcg aagcggtcgt ggcggattcg
     2041 ccggtgacga cgcccgaggt ggtgcaggtc ccgaacctct cgcgcgtggc gccctcgctc
     2101 gccgacgtcg agaacgagac caaggcggcg cggcctcggc tcgttctcta cctcgcggcc
     2161 gccgcgttct tgctggtggg cggcatcatc actgccgtga ctggcatcga gctcgtgaag
     2221 ggctatgggt tcaaccgcac cgagcttcgc ttgcttctgc tggcgatctt ggggatttcg
     2281 ctgacgccag cgacgctctg gctgcttcgc atccgtagcc acgtgtggga caagagctcg
     2341 cgcgtgctgg agctgctcgg tcacctccga gccgcggtgc tgagcttcac cgtttcctat
     2401 ggcgtgctcg cgctcttgat gcgcttcttc gacgacttct tggtgcggct catcggccac
     2461 acgaaagtcc agccgatcgg cgcgagctgg cctggatgga gtttggtgct cgccgccacg
     2521 gggctcatgt tggcgctttc taccttggtg cgccgcgcca tggacggcgc cgttcgctcg
     2581 cgagtcggtc gcgccttcgt gcgcaccctc gtcctcggaa tcaccatcgt tgggttcctc
     2641 ggattgctct acttcggttg gcgttggcat gccctctacg ttcacccccc gggcgctcat
     2701 tcgccgatgt gagcgccttc ggtatatcgg aagtcgcgga gctacttcgc gtcgtattcc
     2761 gccactcgac cgcgccgcta atacctttgc gtgaacgacc gttcgactct ttcggtgttc
     2821 atcggcggtc actttgcaag cactggaaac gatgcagacc cattggtgga ggcgcattga
     2881 agccgcgcaa agtgttgatc gtggacgatt cggagacgat tcgccgacaa gttgcggagg
     2941 cgctcgctcc agccggcttc gaggtggttg aggcggcaga cggcgtcgaa ggcctcgagc
     3001 gcgccgcgca gcatgagctt tcgctgatga tcctcgacgt caacatgccg cgcttgagcg
     3061 gcctcgagat gctggaaaaa ctcaaacgcg acccccgcgg cgcgaagatt ccggtactga
     3121 tgctaactac cgaggttcag cagtcgatgg tcgatcgcgc gaagaagtcg ggggctatcg
     3181 gctggatgat aaagccggtg aagctcgact tgctcgtcag caccgtcaac aaggccgtcg
     3241 gaacctagtc ggagcgcgag ctggtgcttt cagagaacga tgctgtctcc gcgcgcgtcg
     3301 acacgctcac gacgcgggcg tgtgaagagc tcttcgcctc ttacggcgtg gcgttgaccc
     3361 ccgtacgcgg cggcttcgag gatgcgaacg agagcgtgct ctccggcgtg atgggcttca
     3421 tcggcacccg cttgcgcgga acgtgcttgc tcgccagcgt gctcggcccg ctcgaggcgt
     3481 cgcgccccgc gggcaacaac tcgcgcgatt ggatcggtga gctcaccaat caactggttg
     3541 ggcgcttgaa gacgaagctt ttggcgcgcg gcgtcgaagt cttccttacc acgccaatcg
     3601 tgctgcgggg cgcctgcgtc caacccatct cgcgcggcag ccgagagccg gtttggttct
     3661 cttccgcgtt cggcaagctc atggtctggg tcgaggtcga gtgtgggcgc gacctgatgc
     3721 tcgcgagcga aatccccctc agcaccgccg gtgatgcggg cgatatcctc atgttttgag
     3781 cctcactcgt tgtcgaccag gcgcacgccg cgacggctct tgtcgagctc ggtcgcggcc
     3841 cggacgcgct cgtagagcgc gacgacgtcg gtcttgatac cgcgcagcgc gatctccggt
     3901 gacgatttgt ccgcggtctt caaaaacagg ttgcccgtcc cgaccaggcg ctgcgcgaac
     3961 ggccgctcta ccgcgtaatc gttcacgcga tagagatcga cctgctcgag ccgctgtgag
     4021 agcacgcccg tctgaacgac gat
//
LOCUS       DASTKS010001103         7629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1961037, whole genome shotgun sequence.
ACCESSION   DASTKS010001103 DASTKS010000000
VERSION     DASTKS010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961037"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFQ35_23295"
     CDS             complement(<1..652)
                     /locus_tag="VFQ35_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933658.1"
                     /translation="MRYAESLEADLGGYTSGFFPHAARPISAYEPLLASERGVAYLLS
                     QRLDERAAGDGRRVGIQSNLALAPDGSAALSLYELLDGVVRGASLEPGVRERWRAISA
                     KIEQKLGFSLEPELFSNMLFVACTGNSSHPYLKRLSSTLLGTFRNSDARGLYHFFTSL
                     RFACDIDCTGVAARARLTAGDIDVATDAGRAALRQITERILRSAAVCDVSAEENDAD"
     gene            complement(888..2150)
                     /locus_tag="VFQ35_23300"
     CDS             complement(888..2150)
                     /locus_tag="VFQ35_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9933659.1"
                     /translation="MSDSRVEARVEEEGHDLGFQLPPPARVSKGVLLGGAALGALVLA
                     VAFVFAYLPRAAQKKALEEHAAAARDAVPKVAVVRAKPVGDVNSLRLPGSVQPLEEAV
                     IYARANGYVRRWLVDIGARVKEGALLAEIDTPELDQELMQARAALAQAQANQLQAQAN
                     RGLAKTRLDRTGKLVEAGVAAQQELDQTQAQSSVGDADVKVAEAAVAAQQANIRRLLD
                     VQSFRKVTAPFDGIITARNIERGSLVTAGNATPLFRLASTDIVRVFVQVPQSIAPSVV
                     VDTPAKVSVREYPGKVFSARVARTAGVLDAVSRTLNTEIRVPNGDGQLLAGMYAQVEL
                     TTSVPHRVLELPSTALMSDAQGLRVAVVTADNKVHLQPVTIERDLGPTVQLSSGIEPD
                     QRVIQIGSVDLVEGERVEVIDKSAPAQH"
     gene            complement(2156..5332)
                     /locus_tag="VFQ35_23305"
     CDS             complement(2156..5332)
                     /locus_tag="VFQ35_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9933660.1"
                     /translation="MWLVRIALQRPYTFVVMALLIVIVGVGTLLRMPTDIFPDIDIPV
                     ISVVFNYGGLNPQEMERRIVSNYERALTTTVNDIEHVESQTLAGVSVIKIFFQPGARI
                     EAATAQVTAISQTVIRQMPPGATPPFIIRYSASNVPILQVALGSETLTEQQLFDFGVN
                     FVRADIATVPGAQIPWPYGGKQRQVMVDIDPSRLYGLGLSPRDVNNALGLGNVILPSG
                     TAKIGPNEYPVVINSSPELLEEIGNIPIRTVRGTTVYIRDVASVRDGNSPQTSMVHVE
                     GHRSVLMSILKLGGASTLDVVSKIRDMLPGTMAKLPKELKATLLFDQSLFVRAAVDGV
                     VREAVIAAGLTALMIFLFLGSWRSTLIVVVSIPLSILVSVILLAAMGHTLNVMTLGGM
                     SLAVGILVDDATVALENIHRNMAQKKPFLRAIVDGAQQIAVPAFVSTLCICIVFVPVF
                     FISGAAKYLFTPLAEAVVFAMMTSYFLSRTLVPTLVRYLLRGEAEAHVSGDHGAAPVS
                     ILGRFFAAFERSFERLRNRYGGLLALALEHRGTIVAGCVGIVAVSLALFPLVGRDFFP
                     SVDAGLIKLHVRGAPGTRIEETERHFSDVENTIRKVIPSDEIQTLIDNIGVPYSGLNL
                     SLSEGALISSADGQIFIALKEDHKPTAEYVRALRATLARQYPQSTFFFLPPDISTQVL
                     NFGLAAPIDVQVVSAPGKDDEAYAVARKLRSQIAAIPGATDVHLAQVPSVPQLNVDVD
                     RTMASQFGLSERDVASDLLVSLSSSGQVAPSYWLDPKRGVQYLVAVQTPQYKIDSMEA
                     LASTPLSTGEGRPQLLSNVARLRRTFTPQNITHYNVSRTFDVQANVDGTDLASVGARV
                     SEIVAKATPTLPRGMALNLKGQVESMESSFKALSYGLCFAVLLVYLLMVVNFQSWLDP
                     LIILMALPGALAGIAWMLFVTHTTFSVPAQMGTIMCVGVATANSILVVTFANDRRKIG
                     DSARDAALAAGMTRLRPVLMTALAMIIGMLPMSLGLGEGGEQNAPLGRAVIGGLAFAT
                     VFTLVFVPVMYALLRKKAPAPELDLEAV"
     gene            6006..6581
                     /locus_tag="VFQ35_23310"
     CDS             6006..6581
                     /locus_tag="VFQ35_23310"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HET9933661.1"
                     /translation="MGDIIAAFHALSAQDPAAERVGDVERPRELVQAERLAEWVMRVQ
                     PDASDALRLAAHCQHIGRFQVPRSSYPEGRSGYLRWRAELARRHAATAEKILREHRAD
                     PQVIDSVKRILLKQNLANDPEVQAMEDALCLSFLEHEFAEFASRHDDEKLVRILRKTW
                     AKMSEAARALALRLPLDERAQQLVAAALQGA"
     gene            6585..7418
                     /locus_tag="VFQ35_23315"
     CDS             6585..7418
                     /locus_tag="VFQ35_23315"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET9933662.1"
                     /translation="MSSLSVSALYVYPVKSCRGISVPRAEIVRRGFARDRRWMVVDAS
                     GRFVTQRDTPDLCLVGTKLLDGGIELSHPRMPSFVLPNALEEGPELDVSVWSHRGLAV
                     KSEDGSAWLSSALKRPVALVYMPERHERQVNPERARRGDLVSFADGYPFLLISEASLD
                     DLNARLSEPLEMPRFRPNIVVRGATPFAEDDWAELRIGNIGFRGVKGCDRCVVTTLDP
                     ETGVAGKEPLRTLSTFRKRDGKVWFGMNLVHDGAGWLEVGAEVEVRRTATSTPPQHTP
                     A"
BASE COUNT         1430 a         2434 c         2487 g         1278 t
ORIGIN      
        1 cgtccgcgtc gttctcctcc gccgagacat cgcacaccgc ggcactacgt aggatgcgct
       61 cggtaatttg gcggagcgcc gcgcggcctg cgtcggtggc gacgtcgata tcacccgcgg
      121 tgaggcgcgc tcgcgccgcg acgccggtgc agtcgatgtc gcaggcaaac cggagcgaag
      181 tgaagaagtg gtatagtccg cgcgcgtcgg agttgcggaa cgtgccgagc agcgtcgacg
      241 acagccgctt gaggtacggg tgcgacgagt tgcccgtgca cgcgacgaac agcatgttcg
      301 agaacagctc gggctccagc gagaacccca acttctgctc gattttcgcg gaaatcgccc
      361 gccaccgctc acgcacgcca ggttcgagcg aagcgccgcg cacgacgccg tcgagcagct
      421 cgtaaaggga caaggccgcg ctcccatcgg gcgccagcgc gaggttcgat tgaatgccaa
      481 cgcgtcggcc gtctccggcc gcgcgctcgt cgagtcgctg agaaagcaag tacgcgacgc
      541 cgcgctcact tgcgagcaac ggctcgtatg ccgagatcgg tcgagccgcg tgcgggaaga
      601 aacccgacgt gtaaccgcct aagtctgcct ccaaggattc cgcgtaacgc atgacgcact
      661 ctccagaagg agctacgtca cccgcaaggt gtgtcttaag cggtcagagc agcagaccac
      721 ttcaccctgt cgggtgccgc gcgtcacaat ccccgcaagt gagcgcgtgt gtcagaccgt
      781 tgacacgcgc aacaaagccg cctcacgcag atgctgcgcg tccgttcgtt tggaatcggt
      841 tcgcattcgg cgaaaaagtg gaattcaggc tcgatctgag actgcgctca atgttgggca
      901 ggcgcgctct tgtcgattac ctccacacgt tccccttcga ccaaatcgac actaccgatt
      961 tggatgacgc gctggtcggg ttcgatgccg gacgaaagct gcacggttgg gcccaaatcc
     1021 cgctcgatcg tgacaggttg aaggtgcacc ttgttgtcgg cggtgaccac cgcgacgcgc
     1081 aacccttgcg catcgctcat gagtgcggtc gacggcagct cgagcacgcg gtgcggaacg
     1141 ctcgtcgtga gctcgacttg agcgtacatg cccgcgagca actgaccgtc gccattcggg
     1201 acgcggatct cggtgttgag cgtccgtgaa accgcgtcga gcacgccggc ggtgcgagcg
     1261 accctggcgc tgaacacctt ccccggatac tcgcgcacgc tgaccttggc gggcgtatcg
     1321 accaccacgc tcggggcgat gctctgcgga acctgaacga agacccgcac gatatccgtc
     1381 gacgcgaggc ggaagagcgg cgtcgcgttc cccgcggtga cgaggctgcc ccgttcgatg
     1441 ttgcgcgccg tgatgatacc gtcgaacggc gccgtcactt tgcggaacga ttgcacgtcc
     1501 agcaaacgcc ggatgttcgc ttgctgcgcc gcgaccgcgg cctcggcgac tttgacgtcg
     1561 gcgtcgccca ccgacgattg cgcctgagtc tggtcgagct cctgctgagc cgcgacaccc
     1621 gcttcgacga gctttccggt gcgatcgagg cgagtcttcg cgagcccgcg gttcgcctgc
     1681 gcttggagtt ggttggcctg cgcttgggcg agcgccgccc gggcctgcat cagctcctga
     1741 tcgagctcgg gagtatcgat ttcggcgagc agcgcgcctt cctttacgcg ggcgccgatg
     1801 tccaccagcc aacgccgcac gtagccgttc gcgcgcgcat agatgactgc ttcctcgagc
     1861 ggctgaacgc tgcccggcaa gcgcagcgag ttgacgtcac ccaccggctt cgctcgcacg
     1921 accgcaacct tcggcacggc gtcgcgcgcg gccgccgcgt gctcctcgag cgccttcttt
     1981 tgagcggctc ggggcaggta cgcaaacacg aacgccacag cgaggacgag cgcacccaag
     2041 gccgcgccgc cgagcagcac gcctttcgac acgcgcgcgg gcgggggcag ctgaaatccc
     2101 aaatcgtggc cttcttcttc gacgcgagct tcgactcgag aatctgacat gggttttaca
     2161 ccgcctccaa gtcgagctcg ggggctgggg cctttttgcg gagcagcgcg tacatgacgg
     2221 gcacgaagac caaagtgaac accgtagcaa aagcgaggcc gccgatcacg gcgcgtccga
     2281 gcggcgcgtt ctgttcgccg ccctccccca agccgagcga catcggcagc ataccgatga
     2341 tcatcgcgag cgcggtcatc agcaccgggc gaaggcgcgt cattccggcc gcgagcgccg
     2401 cgtcacgcgc cgagtctccg atttttcggc ggtcgttcgc gaacgtaacc accaagatgc
     2461 tgttcgcggt ggccacgccg acgcacatga tcgtgcccat ctgcgccggt acgctgaacg
     2521 tcgtgtgagt tacgaacaac atccaggcga tacccgccaa cgcgcccggc agtgccatca
     2581 gaatgatgag cgggtcgagc cacgactgga agttcacgac catcaagagg tacacgagca
     2641 gcaccgcgaa gcagagaccg tacgagagag ctttgaacga gctctccatg ctctcgactt
     2701 gccccttgag gttgagggcc ataccgcgcg gcagagtcgg ggtggccttt gcgacgatct
     2761 cgctcacgcg cgccccaacc gacgcgagat ccgtcccgtc gacgttggct tggacgtcga
     2821 aggtgcgcga cacgttgtaa tgagtgatgt tctgcggagt gaaggttctc cgcaagcgcg
     2881 ccacgttcga caacagctga gggcgcccct cgccggtcga gagcggagtc gacgcgagcg
     2941 cctccatcga gtcgatcttg tattgggggg tttgcaccgc cacgagatac tgaacgcctc
     3001 gcttcggatc gagccagtag ctcggcgcca cttgcccgct cgaggagagc gacacgagca
     3061 agtcgctcgc tacgtcccgt tcactcaacc caaactggct cgccatggtt cggtccacgt
     3121 cgacgttgag ctgcggcacg ctcggaacct gcgcgagatg cacgtcggtc gccccgggga
     3181 tcgccgcgat ttgtgagcgg agctttcgag cgaccgcgta tgcctcgtca tccttgccgg
     3241 gcgcgctcac cacttgaacg tcgatgggcg ccgcgaggcc aaagttcagc acctgggtcg
     3301 agatgtcggg gggcaggaag aaaaacgtcg actgagggta ctggcgagcc aacgtggcgc
     3361 gcagcgcacg cacgtactcc gccgtgggct tgtgatcctc tttcaaggcg atgaagattt
     3421 gaccgtccgc cgacgagatg agcgcgcctt cgctgagcga caggttcaga ccgctgtacg
     3481 gaacgccgat gttgtcgatc agggtctgaa tctcgtcgct cgggatcacc ttgcggatcg
     3541 tgttctccac gtcggagaaa tggcgttcgg tctcctcgat gcgggttccg ggcgcgccgc
     3601 gcacgtgtag cttgatgagc cccgcgtcga cgctggggaa gaagtcgcgc ccaaccagag
     3661 ggaagagcgc gagcgagacg gcgacgatcc cgacgcaccc ggcgacgatg gtcccgcggt
     3721 gttcgagcgc gagcgcgagc aagccaccgt aacggttccg cagccgctcg aagctgcgct
     3781 cgaaggcggc gaagaaacgg cccaagatcg agaccggcgc cgcgccgtga tctccgctca
     3841 cgtgggcctc cgcctcaccc cggagcaggt agcgcaccag cgtcggcacc agcgtgcgcg
     3901 agaggaagta cgacgtcatc atcgcgaaca cgaccgcctc ggcgaggggc gtgaacaggt
     3961 acttcgcggc cccgctaatg aaaaagacgg gcacgaagac gatgcagatg cacagcgtgg
     4021 agacgaaagc gggaaccgcg atctgttggg caccgtcgac gatcgcgcgc aaaaacggct
     4081 tcttttgcgc catgttccgg tggatgttct cgagcgcaac cgtcgcgtcg tcgacgagga
     4141 tgccgaccgc gagcgacatg ccaccgagcg tcatgacgtt cagcgtgtga cccatcgcgg
     4201 cgagcaggat gacggagacc aaaatcgaca gcgggatcga gacgacgacg atcagcgtac
     4261 tgcgccagct accgaggaag aggaagatca tgagcgcggt gagcccggcc gcgatcaccg
     4321 cttcgcgcac cacaccgtcg accgcagcgc gcacgaacag cgactggtcg aacaagagcg
     4381 tggccttcag ctccttcggg agcttcgcca tcgtgccggg gagcatgtcg cggatcttgg
     4441 agaccacgtc caacgtgctc gcgccgccga gcttgagaat gctcatcagc accgagcgat
     4501 gcccttcgac gtggaccata ctggtttggg gcgagttgcc gtcgcgaacg ctggccacgt
     4561 cacggatgta gacggtcgtg ccgcgcacgg tgcggatcgg gatgttgccg atctcttcga
     4621 ggagctcggg gctgctgttg atgacgaccg ggtactcgtt ggggccgatc tttgccgtac
     4681 ccgaaggaag gatgacgttg ccgagcccga gcgcgttgtt cacgtcgcgc ggcgacaaac
     4741 cgaggccgta cagacggctc gggtcgatgt cgaccatcac ctgccgctgc ttgccgccgt
     4801 acggccaagg gatttgtgcg cccggtaccg tcgcgatgtc ggcacggacg aagttgactc
     4861 cgaagtcgaa gagctgctgc tcggtcaacg tttcgctacc gagggcgacc tgcaaaatgg
     4921 gtacgttcga agcgctgtag cggatgatga acggcggagt cgcgccgggc ggcatctgtc
     4981 gaatcacggt ctgggaaatc gccgtgacct gcgcggtcgc ggcttcgatg cgagcgcccg
     5041 gctgaaagaa gattttgatg accgaaacac cggcgagcgt ctggctctct acgtgttcga
     5101 tgtcgttcac cgtcgtcgtc agcgcgcgct cgtagttgct gacgatgcgg cgctccatct
     5161 cttgcgggtt gaggcccccg tagttgaaca cgacggagat caccgggatg tcgatatcgg
     5221 gaaagatatc ggtcggcatc cgcagcagtg tgcccacgcc gacgatgacg atgagcaacg
     5281 ccatgacgac gaaggtgtaa gggcgctgga gggctattcg aacgagccac atggcttatt
     5341 gcggagtagc gtctgatcgc ggtgaaggtt gaagtgttac gagcgagacg cggaaacgta
     5401 ggactcagcg gcgcatcgtc caatggggca aaacggcgag cgctccatgg gcgaatttgc
     5461 cacccccgcc ttgcgagccg cgcgccctaa accgcggagc gacccagccg aggggccacg
     5521 gcgtttagaa gcttggcgcc ccgcgcctcg agcgacgaac tcagcagctc gccgggttcg
     5581 cgccaggtgc aggcttacac ccaagctcac gcgagttaac ggaacgatga aacgccgaac
     5641 cacagaccgc caaacgggcc gcatctgcgg tcctggccgc cgcgggccgc gaatcttgcg
     5701 ggccgcccga gcggttgctc gtgttgttag catgacgcga gggaacgttg gctctccgaa
     5761 acgtgtctaa ccgtcccggt tcccccatgt tcgccgcctc gaggctcgtt ccggaaacct
     5821 cttcgagcgc cgtgcggcgt gaaagcgcgc gcaatcgtac gaaattggcg aaactgagcg
     5881 gcgcgcccga gccgcacacc ttaccgcttc tcgaacgccg tctcccccga caaatccgcg
     5941 tggcactgcg acgcttgccg ccgcgagggc tttccgttag cgaaatccgc atggaacgag
     6001 cgcagatggg cgacattatc gccgcgtttc acgcgttgag cgcgcaagac cccgccgccg
     6061 aacgggtagg cgacgtcgag cgtcctcggg agctcgtgca agcggagcgc ctcgccgaat
     6121 gggtgatgcg cgtgcaaccg gatgcctcgg acgcgctgcg tcttgccgcg cattgccagc
     6181 acatcggtcg atttcaggtc ccgcgctcga gctatccgga aggccgaagt ggttacctgc
     6241 gttggcgcgc agagctcgcg cggcgccacg cggcaaccgc cgaaaagatt ttgcgggagc
     6301 atcgagctga cccgcaagta atagacagcg tgaagcgcat cctgctgaag cagaacctgg
     6361 ccaacgatcc agaagtgcaa gccatggagg atgcgctctg tctctcattc ctcgagcatg
     6421 agttcgccga gttcgcgagt cgccacgacg acgagaaact cgtgcgcatc cttcgcaaga
     6481 cctgggcaaa aatgtccgag gccgcccgcg ccctcgccct tcggctgcca ctcgacgaac
     6541 gcgcccagca actcgttgcg gcagcgctgc agggggcctg aacgatgagc agcctttcgg
     6601 tcagcgcgct ctacgtgtac ccggtgaaga gttgccgcgg gatctccgta ccgcgagcgg
     6661 agattgtacg gcgcggcttt gcgcgagacc gtcgctggat ggtggtcgac gcaagcggca
     6721 ggttcgtcac gcagcgggac acccccgatc tctgtctcgt cggcacgaag ctcctcgacg
     6781 gtggtatcga gctctctcac ccgcgcatgc cgtcattcgt gctaccgaac gcgctcgagg
     6841 agggacccga gctcgacgtg agcgtgtggt cacatcgggg gctcgcggtg aagagcgagg
     6901 acggcagcgc gtggctgagc tccgcgctga agcgcccggt cgcgctcgtc tacatgccgg
     6961 agcgccacga gcggcaggta aaccccgagc gcgcgcggcg cggtgacttg gtcagcttcg
     7021 cagacggcta ccccttcctg ctgatctcgg aagcgtcgct cgacgacctg aacgcgaggc
     7081 tcagcgaacc gctcgagatg ccgcgctttc gtccgaacat cgtggtgcgc ggcgcaacac
     7141 ccttcgccga ggacgactgg gccgagctcc gcatcggcaa catcggcttt cgaggcgtca
     7201 aaggctgtga ccgttgcgtg gtcacgactc tcgatcccga aacgggcgtc gccggcaaag
     7261 agccgctccg cacgctatcg acgttccgca agcgagacgg caaggtgtgg ttcggaatga
     7321 acctcgttca cgacggcgca ggctggctgg aggtcggcgc ggaggtggaa gtgcgccgaa
     7381 cggcgacatc gactccacca caacacacgc ccgcttgagc catcagctct cgggtatgac
     7441 ctcgagatcc gaatcgcgac tagagccgcg cggtcgcacg cattttctcg aggcgttgcc
     7501 gtcggcgcgc tcggagccaa ccgaagggag tgaggacgta agtaaagcat ccgtacgcat
     7561 tctcaattcg gtacgaatcg aaattggaga tcgcgagtaa atagtggttc aagggttgcg
     7621 ctgactcgc
//
LOCUS       DASTKS010001104         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_395137, whole genome shotgun sequence.
ACCESSION   DASTKS010001104 DASTKS010000000
VERSION     DASTKS010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395137"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VFQ35_23320"
     CDS             complement(<1..1417)
                     /locus_tag="VFQ35_23320"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAHL domain-containing protein"
                     /protein_id="HET9933663.1"
                     /translation="MNAAALVRVVFALSALGLALVGIQRYRLSIDHARVAAYGYDLRQ
                     LSVFDARLNEELLSSRSGLVSHYDGLSRSAADVKNSLKRLSSPPTFVQGAEAAELRAR
                     LRELSRLFERKQVPLEGFKSHNAVLRNSRAFLPVAGRRLLARAEQNGVLARRVGEVLA
                     WFWGNDGALRGDSSELDATLDALRRIGADARDPALSDEIEVLLRHTLVVRARQAIVDE
                     LVREALALPFGRSASRLEGVYAKAYRGALVAEERREQLLFALAMVVVASGLSDMLLRV
                     RKSARALELATAELTSVNQALAVEREKERELGDMKSRFVSMTSHEFRSPLSAVLSSGE
                     LLSRYGDRWPSEKREEHLSRIRSSARQMSQLLDEILLIGTAEAGTLRPTPAHLNLDEL
                     SQDLVQTLEQGLEKPVVISRTLNGDPNVFLDEKLLRHLLWNLLENAVKYSSEGSEVKL
                     AIVVDPASVRFSVRDTGIGIPE"
     gene            complement(1414..>2213)
                     /locus_tag="VFQ35_23325"
     CDS             complement(1414..>2213)
                     /locus_tag="VFQ35_23325"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9933664.1"
                     /translation="VSEDGRVSCGSCHPRERAFVDGNDRTEIEGRPRTPTNTTTLLNV
                     RYYYWLGWFGQSDSLEGHLDGLIKSRKLMASSWDGITGRLQADPKWVRRFSAVFSDGV
                     TSDNVRRALLAYERSLTTPNAPFDRFLRGEAEALSSEARQGYSLFKSFGCVSCHQGVA
                     VGANMLETFGVMRDYFADRGERSDADRGRMNVTGRDEDRHVFRVPSLRNVAVTAPYFH
                     DGSAPTLDEAVKVMARYQLGRELDPNEIGAIVAFLGSLTGEYPGDSR"
BASE COUNT          393 a          751 c          712 g          357 t
ORIGIN      
        1 cttccggaat cccgatgccg gtgtcccgca ccgaaaagcg gacgctagcc gggtcgacca
       61 cgatcgcaag tttcacctcg ctgccttcgg aagagtactt caccgcattt tcgagcaggt
      121 tccagagcag gtggcgcagc agcttctcgt cgaggaacac gttcggatca ccgttcagag
      181 tccgcgagat gacgacgggc ttttcgagcc cttgctcgag cgtttggacc agatcctgag
      241 agagctcgtc gagattcaag tgggcaggcg tcgggcgcaa ggtgccggcc tcggcggtgc
      301 cgatgagcag aatctcgtcg agcaactggc tcatttgccg tgccgagctg cgaattcggc
      361 tcaggtgctc ctcgcgtttc tcgctgggcc accggtcgcc gtagcgcgag agtagctcgc
      421 cagaagagag caccgcgctg agcgggctgc ggaactcgtg cgaggtcatg gatacgaagc
      481 ggctcttcat gtcgccgagc tcgcgctcct tttcgcgttc gaccgcgagc gcttgattca
      541 cgctcgtaag ctccgcggtc gcgagctcga gcgcgcgagc actctttcgc actcgcagga
      601 gcatgtcgct cagacccgag gctaccacca ccatcgcgag cgcaaagagg agctgttccc
      661 gacgttcctc ggccaccagc gcaccccgat acgccttcgc gtacacgcct tcgaggcgag
      721 acgcgctgcg cccgaacggg agcgccaacg cttcccgcac gagctcgtcc acgatcgctt
      781 gccgagcccg cacgacgagc gtgtgtcgga gcagcacctc gatctcgtcc gagagcgcgg
      841 gatcgcgcgc atcggccccg atgcggcgca gggcgtcgag ggtcgcgtcg agctcactcg
      901 aatcgccacg cagggccccg tcgttccccc agaaccatgc gaggacctcg cccacccgcc
      961 gggccagcac accgttctgc tccgctcgcg ccagcaagcg ccgccccgcc accggcaaga
     1021 acgcgcgcga attgcggagc acggcgttgt ggctcttgaa cccctcgagc ggaacttgct
     1081 tgcgctcgaa caggcgcgaa agctcgcgca gccgcgcgcg gagctccgct gcttcggcgc
     1141 cctgcacgaa cgtcggcgga gacgacaagc gcttcagcga attcttcacg tcggcggcgc
     1201 tgcgcgaaag tccgtcgtag tggctcacga ggccggagcg ggagctcagg agctcttcgt
     1261 tgagtcgagc gtcgaacacg ctgagctgac gcaggtcgta gccgtaagcg gcgacgcgcg
     1321 cgtggtcgat ggacaaccgg tagcgctgga tgccgaccaa cgcgagaccg agcgcgctca
     1381 gcgcgaacac gacgcgcacg agagccgccg cgttcaccgc gagtctcccg gatactcccc
     1441 cgtcagggag cccaagaagg cgacgatcgc gccgatttca ttcggatcga gctcgcgccc
     1501 gagctgataa cgcgccatca ccttcaccgc ctcgtccagc gtcggggcag agccgtcgtg
     1561 gaagtagggc gcggtaaccg cgacgttgcg caggctcgga acccggaaca cgtgacgatc
     1621 ctcgtcacgc ccggtcacgt tcatgcgccc gcgatcggcg tcgctgcgtt caccccgatc
     1681 cgcgaagtag tcacgcatca cgccgaacgt ctcgagcatg ttcgcgccca ccgccacgcc
     1741 ctggtgacag ctgacgcacc cgaagctctt gaagagcgag tagccctggc gcgcctcgga
     1801 cgacaacgcc tccgcttcgc ctcgcaagaa gcggtcgaag ggcgcgttgg gcgtggtgag
     1861 cgatcgctcg tacgcgagca gagcgcggcg aacgttgtcc gaagtgacgc cgtcggagaa
     1921 cacggccgag aagcgcctca cccacttcgg gtcggcttgc aggcggcccg taatgccgtc
     1981 ccagctgctc gccatgagct tgcgactctt gatgaggccg tcgaggtgcc cctcgagcga
     2041 gtcgctttgt ccgaaccaac caagccagta ataatatcga acgttgagga gggtcgtggt
     2101 gttggtcggc gtgcgggggc gcccttcgat ctcggtgcgg tcatttccgt cgacgaaggc
     2161 gcgctctcgg ggatgacagc tgccgcaaga aacgcgacca tcctccgata cga
//
LOCUS       DASTKS010001105         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_173608, whole genome shotgun sequence.
ACCESSION   DASTKS010001105 DASTKS010000000
VERSION     DASTKS010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173608"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFQ35_23330"
     CDS             <1..1033
                     /locus_tag="VFQ35_23330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9933665.1"
                     /translation="GLAQASGPVVLAWGEWVAEHLALEAFVEQAGTVAAGTMRTLTVE
                     GADAPFPAVVLGDELRERAATLPPESFADAPTFILALKRAANPEVEPKTVFEGYFAKI
                     TDAETAKSASFRLLERLRWRPGGLVAKYLNRPISIRLSYLLLDTKVTPNQTTIFAFLV
                     GAVGVVFVFLGGYQNTIIGTALMQANSVIDGIDGELARIRHQNSEYGAYLDSVCDEIL
                     NAALMIAVGYNLSTYVHHNPLYMILGTFAGLVSFFYASVHWHCKAKHGLGFYWWFEAY
                     KPRREVQRSTSTWSYLKKLTMKESYLLLFLLAAIFGGLEALLWIFAAVGVQVLVLFTI
                     HIPIKRARW"
     gene            complement(1050..2063)
                     /locus_tag="VFQ35_23335"
     CDS             complement(1050..2063)
                     /locus_tag="VFQ35_23335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9933666.1"
                     /translation="MSTPKVSVLIPAYNAERTLEATLRSVLRQTLQSWECVVVDDGST
                     DRTAALLHEYARRDARVVATTSEHLGIVGALNKGLERCRGDYVARLDADDVMHRLRLE
                     RQYAFARCEPEIGALGCHVRIFPRRAGSGGRRGYERWLNSLVDEASLLRDRFVECPLA
                     HPSFFVRNDVFAAHPYRDVPWAEDYDLLLRLQESGVRVGVVPEVLLFWREGPTRLSRV
                     DSRYSIERFVECKAHFLSRRFLADESGYVLWGYGDTGRALARALRGHRKHPTHIVELH
                     PGRVGQRIANAPVIAPIALRELTPRPHRIVVSVAGEAARSEIRARLAEMRFEEGRDYV
                     CAA"
     gene            complement(2067..>2933)
                     /locus_tag="VFQ35_23340"
     CDS             complement(2067..>2933)
                     /locus_tag="VFQ35_23340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9933667.1"
                     /translation="QRLDGGAMFGNAPRALWTRWLAPDELGRVPLACRAFLVDDGEQR
                     VLIETGIGAFFEPSLRERYGVVEDRHVLLESLASLGVDANDVDWVVLSHLHFDHAGGL
                     LAPYRAGEPPRLAFPKARIVTSRVAFERAEHPHARDRASFIPELPALLKSSGRLELVE
                     RGEELTQLLGSRFEASITQGHTVGMLHTWVAGAHGSAFFCADLVPGSAWVHLPVTMGY
                     DRFPELLIDEKAALFELIRGRGAHCLFTHDSSFAAARLERDGKGRYTVTGAVPEFRDW
                     DLDASTAPTGTA"
BASE COUNT          496 a          951 c          988 g          498 t
ORIGIN      
        1 cggactcgct caagcatcgg gcccggtcgt gctcgcctgg ggtgaatggg tcgccgagca
       61 tctcgcgctc gaagcgttcg tcgagcaagc gggcacggtc gcggcgggca ccatgcgcac
      121 gctgacggtc gagggggcgg acgcgccgtt cccggcggtc gtgctcgggg acgaactcag
      181 ggagcgcgcc gcgacgctgc cacccgagtc gttcgccgat gctccgacct tcattcttgc
      241 cctcaagcgc gccgcgaacc ccgaggtcga gcccaagacc gtattcgagg gttacttcgc
      301 caagattacc gacgcggaaa cggcgaagtc ggcgagcttc cgcctgctcg agcgcttgcg
      361 ctggcgtcca ggcgggctcg tggccaagta cctcaatcgg ccgatttcga ttcggctgag
      421 ttacctactg ctcgacacca aagtaacgcc gaatcaaacg accatcttcg ccttcttggt
      481 cggcgcggtc ggcgtcgtct tcgtgttcct gggtggctac caaaacacga tcatcggtac
      541 cgcgctgatg caggcgaatt cggtgatcga cggcatcgac ggggagctcg cgcgcattcg
      601 ccaccaaaac tcggagtacg gcgcttacct cgactccgtt tgcgacgaga tcttgaacgc
      661 ggcgctgatg atcgcggtgg ggtacaacct ttccacctac gttcaccaca atcccctgta
      721 catgatcttg gggacgttcg ccggtctcgt ctcgttcttc tatgcgtcgg tgcattggca
      781 ctgcaaggcg aagcatggcc tcggatttta ctggtggttc gaggcgtaca aaccgcggcg
      841 cgaggtgcag cgcagcactt cgacttggag ctacctgaag aagctcacga tgaaggagtc
      901 gtatctcctt ctgttcttgc tcgcggcgat ctttggcggc ctcgaggcgt tgctgtggat
      961 cttcgcggcg gtgggcgtgc aggttctcgt cctgttcacg attcacatcc cgatcaagcg
     1021 cgcgcgctgg tgaaaggcgg cgtggcgctt caagcggcgc agacgtagtc gcgtccctct
     1081 tcgaaccgca tttcggctag ccgggcgcgg atctcgcttc gcgcggcctc tcccgcgacc
     1141 gacactacga tgcggtgagg ccgcggggtg agctcacgca gcgcgatggg ggcgatcacc
     1201 ggggcgttcg caatgcgttg gccgacgcga cccgggtgca gttcgacgat gtgtgtcggg
     1261 tgttttcgat ggccccgcag cgcgcgcgcc agggcgcgac cggtgtcgcc gtagccccaa
     1321 agcacgtagc cgctttcgtc cgcgaggaac ctgcgcgaca aaaagtgcgc cttgcactcg
     1381 acgaagcgct cgattgaata gcgcgagtcg acgcgagaga gtcgcgtcgg tccttctctc
     1441 cagaacaaga gcacctccgg cacgacgccg acgcgcaccc cgctctcctg gagacgaagc
     1501 aagaggtcgt agtcttcggc ccacggcacg tcgcggtagg ggtgcgccgc gaagacgtcg
     1561 tttcgcacga agaagctcgg atgggcaagc gggcactcga cgaagcggtc ccgcagcagc
     1621 gacgcctcgt cgacgagcga gttgagccag cgttcgtagc cacgtcgccc cccgctcccg
     1681 gctcggcgcg gaaagatgcg aacgtgacag ccgagcgcgc cgatttcagg ttcgcagcgc
     1741 gcgaaggcgt actgtcgttc gaggcgcagc cggtgcatca cgtcgtccgc gtcgaggcgc
     1801 gcgacgtagt ccccacggca gcgctcgagc cccttgttga gcgccccgac gatgccgagg
     1861 tgttcgctcg tggtcgcgac gacgcgcgcg tcacgccgcg cgtactcgtg aaggagcgcg
     1921 gcggttcggt cggtagagcc atcgtcgacg acgacgcatt cccagctttg caaggtttgc
     1981 cggagcacgc tgcgcaacgt cgcctcgagc gtccgctcgg cgttgtacgc gggaatgagg
     2041 accgagactt tgggcgtgct catccgctag gcggtgccgg tcggcgccgt gctcgcgtcg
     2101 agatcccaat ctcgaaactc tgggaccgcg ccggttaccg tgtatcggcc cttcccgtcg
     2161 cgttcgagcc gcgccgccgc gaacgagctg tcgtgcgtga acaggcagtg cgcgccgcga
     2221 ccgcgaatca gctcgaagag cgcagccttt tcgtcgatca aaagctcggg gaagcggtcg
     2281 taccccatcg tgactggcag gtggacccag gcgctgcccg gcacgagatc ggcgcaaaag
     2341 aaggctgacc cgtgcgcgcc cgcgacccac gtatgcagca tgccgacggt gtgcccctgc
     2401 gtaatgctcg cttcgaagcg cgacccgagg agctgagtga gctcctcgcc gcgctccacg
     2461 agctcgagcc gcccgctcga tttgagcagc gcggggagct ccgggatgaa cgaagcgcga
     2521 tcgcgcgcgt gcggatgctc ggcccgctcg aaggcgaccc ggctcgtcac gatgcgggcc
     2581 ttggggaagg cgaggcgcgg cggctccccc gctcgataag gagcgaggag gccgcccgcg
     2641 tgatcgaagt gcaaatgact cagcacgacc caatcgacgt cgttcgcgtc gacgccgagc
     2701 gacgcgagcg actcgagcaa tacgtggcgg tcttcgacca cgccgtagcg ctcgcgtagg
     2761 ctgggctcga aaaaggcacc gatgccagtc tcgatgagca ctcgctgttc gccgtcgtcg
     2821 accaaaaagg cgcgacaagc gaggggaacg cggccgagtt cgtcgggcgc gagccaacgg
     2881 gtccagagag cgcgcggagc gttgccgaac atggcgccgc catcgagccg ctg
//
LOCUS       DASTKS010001106         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1066488, whole genome shotgun sequence.
ACCESSION   DASTKS010001106 DASTKS010000000
VERSION     DASTKS010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066488"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2233
                     /locus_tag="VFQ35_23345"
     CDS             <1..2233
                     /locus_tag="VFQ35_23345"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET9933668.1"
                     /translation="LQLGAGDRVSADVKVGGGRGLFVDNSNLTGESVPVPVSSGELAF
                     AGTFVVEGEAQALVCATGRGTRLARIAELTQAHTRPRTPLARELSRLVRVVAVVAFLV
                     GLLFFAVGALVGLPAREGFLLAIGVAVALVPEGLLPTVTLSLAMGAARMASRGALVRR
                     LDAVETLGATTFICSDKTGTLTTNEMNAVEVWTPVGTTAVRGSGYAPSAEIEGVEEAR
                     KAAKQLALVASRCSSGRVLERDGNWVARGDPMEAALHALALRFGVDVESSERAAPERL
                     RFPFDPRRRRMSVVLDEDVFVKGAPDSVVPFVREPGDATCAAAEMASRGLRVIAVARR
                     KVISHERVESAEAAEDELTMCGLVGLLDPPRPEARRAIELCRKAGMRVAMVTGDQEGT
                     ARAIAKEVGLYPELVVKGAELPSDLAALGAVMDRDGVIACRVSPEQKLEIAKALQARG
                     HVVAMTGDGVNDGPALQQADVGVAMGRSGTDVARAAADLVLLDDRFETIVDAIEQGRS
                     TFSNIRRFLTYHLTDNVAELTPFALWALSGGRFPLALGVLQILCLDLLTDQLPALALG
                     AEPPSPRTLSGPPRRTHLIDRALLLRAFAVLGPTESLIAMAVFALTLRAFGWQLGAAP
                     PKGDALAAASAGAFVAIVFGQSATALCCRSGSRSIFRVPPRTNPRLLGALAVTWGVVL
                     ALLFVPWFARPLGHAPPPTGTWVMAALAFPAVVVVDTLFKRWWRERGDNSAGELTSRA
                     RIV"
     gene            2236..3117
                     /locus_tag="VFQ35_23350"
     CDS             2236..3117
                     /locus_tag="VFQ35_23350"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9933669.1"
                     /translation="MNRSVGCLVGAIPDPTQGVELPAWFLYPSHAIERIEHFGPYPFS
                     AALGGPPVGSGWPLVAISHGNNGSPWTHRETARALVHAGYAVVMVEHLGNSRSDASLA
                     NTSHNLTNRPRHLRLALDWATLHCEHREHVSTTDVFGLGHSIGAYTILASAGGEPSMS
                     HGPGRIGPRVPVEHDARLRALVLLAPAAPWFIPEGSLENVELPMLLWRGELDSLAPEF
                     HSEVILRGVRRRELVDYRVASGAGHFSFQSPFPASMVSREFPPSQDPEGFDRAGFQSA
                     LNREIVAFLERQRRPRP"
     gene            complement(3127..3294)
                     /locus_tag="VFQ35_23355"
     CDS             complement(3127..3294)
                     /locus_tag="VFQ35_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933670.1"
                     /translation="MKLNEPDWGDFVQNACLRLTVALFAIYMLFVCGSAHAFDLSDGA
                     QATHGDAAPRP"
BASE COUNT          515 a         1146 c         1178 g          628 t
ORIGIN      
        1 gcttcaactc ggcgcggggg atcgcgtcag cgcggacgtg aaggtggggg gcgggagggg
       61 gctcttcgtc gacaactcga acttgacggg agagagcgtg cccgtccctg tttctagcgg
      121 agagttggcg tttgcgggta cgttcgtcgt cgagggagaa gcgcaggctc tggtgtgcgc
      181 gacggggcgc ggcactcgcc tcgctcgaat cgcggagttg actcaagcgc acacgcggcc
      241 gcgcacgccg ctggcgcgcg agctgagccg gttggtgcgc gtcgtcgcgg tggtggcgtt
      301 cctagtcggg ttgctgttct tcgccgtggg cgcgctcgtg gggctgccgg ctcgcgaggg
      361 ttttcttctc gcgattggcg ttgcggtcgc gctggtgccc gaggggctac taccgacggt
      421 gacgctgtcg ctcgcaatgg gcgccgctcg catggcttcg cgcggcgcgc tcgtgcgacg
      481 tctggacgcg gtcgagactc tcggcgcgac gacgttcatc tgcagcgaca agaccggcac
      541 gttgacgacg aacgagatga acgcggtgga ggtctggacg ccggtcggaa cgacggccgt
      601 gcggggtagc ggttacgcac cctccgccga aatcgaaggc gtggaggaag ctcgcaaggc
      661 ggcaaagcag ctcgcgctcg ttgcgagtcg ttgctcgagc gggcgcgtcc tcgagcgcga
      721 cgggaactgg gtcgcgcgcg gcgacccgat ggaggcagcg cttcacgctc tcgcgctgag
      781 gttcggcgtg gacgtcgagt cgagcgagcg cgccgcgccg gagcgcctgc gtttcccttt
      841 cgatccgcgg cgccgacgga tgtcagtggt gctcgacgag gacgtcttcg tcaagggcgc
      901 tccggactcg gtggttccgt tcgtgcgcga gcccggcgac gccacttgcg cggcggccga
      961 aatggcttcg cgagggctac gggtcatcgc cgtcgctcgc cgcaaggtca tcagccacga
     1021 acgcgtcgag agcgctgaag cggccgaaga cgagctcacg atgtgcggcc tcgttgggct
     1081 cttggacccg ccccggcccg aagctcgtcg ggcgatcgag ctctgccgta aagcgggaat
     1141 gcgggtcgcg atggtcaccg gtgatcaaga gggaactgca cgcgctatcg cgaaagaggt
     1201 gggactatac ccggagctcg tggtgaaagg agcagagctc ccctccgacc tcgcggccct
     1261 gggcgcggtg atggatcgtg acggcgtcat cgcgtgccgc gtctctcccg aacagaagct
     1321 cgagatcgcg aaggcactgc aagcgcgcgg tcacgtcgtc gcgatgacgg gggacggggt
     1381 gaacgatggc ccggcactcc agcaagccga cgtcggggtc gccatgggac gctccggtac
     1441 ggacgtagcc cgcgccgcgg ccgacctcgt cctgctcgac gaccgcttcg agaccatcgt
     1501 cgacgccatc gagcaggggc gaagcacctt ctcgaacatc cggcgctttc taacgtatca
     1561 cctgaccgac aacgtcgccg agctcacgcc gttcgcgctc tgggccctct ccggagggcg
     1621 cttcccgctc gcgctcggag tgcttcaaat cctgtgcctc gacctgctga cggatcagct
     1681 gcctgccctc gcgctcggcg ccgagccgcc tagcccacgc acgctgagcg gccctccgcg
     1741 gcgcacgcac ctcatcgatc gtgcgctgtt gctacgcgcc tttgccgtgc tcgggccgac
     1801 cgaatcgctg atcgcgatgg ctgtcttcgc cctcacgttg cgcgccttcg gctggcagct
     1861 cggcgcggcg ccacctaaag gcgatgcgct cgcggcggct tcggcgggtg cgttcgtcgc
     1921 gatcgtcttc ggtcagtccg cgaccgcgct ctgctgtcgc agcggctcgc gcagcatctt
     1981 tcgtgtgccg ccgcgcacca atcctcggct gctcggcgcg ctcgccgtca cgtggggcgt
     2041 cgtgctcgcg ctgttgttcg tgccgtggtt tgctcgaccc ctcgggcacg cgccgccgcc
     2101 gacgggaacg tgggtgatgg ccgcgttggc attcccagcc gtcgtcgtcg tcgatacgct
     2161 cttcaagcgg tggtggcggg agcgaggcga caattccgct ggcgagctca cgagtcgtgc
     2221 gcgtatcgtc tagcggtgaa ccgctcagtt ggttgcctcg tcggtgcgat tccggatcca
     2281 acccaagggg tcgagctccc tgcgtggttt ctctacccgt ctcacgcgat cgagcgcatc
     2341 gagcacttcg gcccctatcc cttcagcgcg gcgctcggcg ggcctccggt cggttcgggt
     2401 tggcctctcg tcgcgatcag ccacggcaac aacggcagcc cctggacaca tcgggagacc
     2461 gcgcgcgccc tggtccacgc cggttacgca gtggtcatgg tcgagcacct cggcaatagc
     2521 cggagtgacg cgagcctcgc caataccagc cataacctca cgaatcgacc acgtcacctg
     2581 cgcctcgcgc tcgattgggc aacccttcac tgcgagcatc gcgagcacgt ctccacgacc
     2641 gacgtgttcg ggctcgggca ctcgatcggt gcttacacaa tccttgcgtc ggcgggcggt
     2701 gagccgtcga tgtctcatgg tcccggtcgt atcggtccgc gtgtccccgt cgagcacgac
     2761 gcgcgcctac gggcgctcgt attgctcgcg cctgcggctc cttggttcat tcccgagggc
     2821 tcgctcgaaa acgtcgaact tcccatgttg ctctggcgtg gggagctcga ctcgttggct
     2881 cccgagttcc attcggaagt cattttgcgc ggcgtacgga gacgagagct cgtcgactat
     2941 cgcgtcgcat ccggcgccgg ccacttctcg ttccagagcc catttcccgc tagcatggtg
     3001 agtcgcgagt tcccaccgtc acaggacccc gagggcttcg accgagctgg attccagagc
     3061 gcgctcaacc gcgaaatcgt cgcgttcctg gagcgccagc gccgcccgcg cccttagcta
     3121 tcgcggtcag ggccgcgggg cagcgtcgcc gtgcgtagct tgtgcgccgt cggacaagtc
     3181 gaaggcgtgt gcgctaccgc aaacgaacag catgtagatg gcgaagagag ccaccgtgag
     3241 tcgcaagcaa gcgttctgca cgaaatcacc ccagtcaggc tcattcagct tcacggaaca
     3301 ctccttggcc cacccgccca gctctcagag agagccgtga tcggccgatt cagatgtagc
     3361 ctgaggtaag tgtatcggca acgtctgaat acatggtgcg ccgcgtctac gcactgcaga
     3421 cgactgcaat cgttggtgat tcgatgaacg accgcaatga gcgagtc
//
LOCUS       DASTKS010001107         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1066546, whole genome shotgun sequence.
ACCESSION   DASTKS010001107 DASTKS010000000
VERSION     DASTKS010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066546"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFQ35_23360"
     CDS             <1..360
                     /locus_tag="VFQ35_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933671.1"
                     /translation="HSLIPNRAGRVGLAGEPVARSYVVGWIKRGPVGLLGTNKQDARE
                     TVAAMLEDAPSALGERSKRTPGSALQRLDERGVRRVTYADWQKIDAQERKLGAAMGKI
                     REKFASVAELLQAIESG"
     gene            420..944
                     /locus_tag="VFQ35_23365"
     CDS             420..944
                     /locus_tag="VFQ35_23365"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET9933672.1"
                     /translation="MKRISATLAALFFAGSFAVAASAQEPAAHDHEASTSVNFDKYVP
                     GAAELEGRIIAPCCWTQTIEIHGSPASTELRQEIRQRLTAGETPDAIERSLVQRYGPK
                     ILAVPPGSRLPSVGIGLGLVVIGAGIGALTLLKRWQRRATPALADGPKLEPGAADAEL
                     DARVDAELSRLDRE"
     gene            941..2023
                     /locus_tag="VFQ35_23370"
     CDS             941..2023
                     /locus_tag="VFQ35_23370"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HET9933673.1"
                     /translation="MNKLELPIITARGSARELGHAQGEALRESIAAFVDQRLSALRAY
                     LAERNESARYSEFVAVGERCLAIARTFDPLGTEEHEAIAEAAHVAPELLYAATNMTDV
                     RDVLVLPSTPDREGCTSLLVPAERARGKQLLAGQTWDLNPTDLDFVVAVHRLPNEGPE
                     TWSVTCVGALSLVGMNELGVAVGTTNIKTRASRPGVGYLSILHKAVTRRSYEDAVAVV
                     RSAPRAAAHTYWVASAAQALELECDPELVVERPLLADAVVQTNHCQAAPLAAREGEAP
                     SASSQRRLARAGVLLARSDQDFSSVEALFADRADGVDSINRYSEDAQGTTTNACFVCC
                     PATREIRACRGPADRGEWRSLAFERG"
     gene            complement(2028..>2930)
                     /locus_tag="VFQ35_23375"
     CDS             complement(2028..>2930)
                     /locus_tag="VFQ35_23375"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9933674.1"
                     /translation="DRREIRDILSYLRVLSNPSDEVSLLRIINTPARGIGTSTIEGLL
                     EFAVSEGKPLWNALPLALEVLELPTSTITRIEGFRKLIDGFRAKLGQVPISELMRELL
                     QTIEYRTELERVHTGPGEAEARWQAVEELVNAAALYEQRETSPTLLGFLEEATLSDRD
                     FGEDDKRERHAVTLMTLHSAKGLEFPHVFMVGLEEGILPHQRSVMEGRGVDEERRLCY
                     VGVTRAQETLTITFAKARMKWGKPRPSIPSRFLMEMRGDTERAKKAAEAADALFRKGA
                     DGSGDAAEAEPKSKKKPRIPLKRK"
BASE COUNT          451 a         1008 c          990 g          481 t
ORIGIN      
        1 cactcgctca tcccgaatcg ggccggtcga gttgggctcg cgggcgaacc ggtcgctcgc
       61 tcttacgtcg tcggctggat caagcgcggg cccgtcgggc tgctcggcac caacaagcaa
      121 gatgctcgcg agacggtagc ggccatgctc gaagatgccc ccagcgctct cggcgagcga
      181 tcgaagcgta cgcccggctc tgcgttgcag cggctcgacg agcgcggagt ccgccgggtg
      241 acctacgcgg actggcagaa gatcgacgcc caggaacgca agctcggcgc cgcgatgggc
      301 aagattcgcg agaaattcgc gagcgtggcg gagctcttgc aagccatcga gagcgggtga
      361 gcggcgccgc gggacgccgc ctcgtcgagc cgcggcgtct gtggcaagct cctctcgccg
      421 tgaaaagaat atccgccacg ctcgctgcgc tgttcttcgc aggctctttc gccgtcgcgg
      481 cgtcggcgca agaacccgca gcgcacgatc acgaggcttc gacgagcgtc aacttcgaca
      541 agtacgtccc gggcgcggcg gagctcgagg ggcgcatcat cgcgccgtgc tgttggacgc
      601 aaacgatcga gattcacggc tcgcccgcat cgaccgagct gcgccaggag atccgtcagc
      661 ggctcaccgc cggcgaaact cctgatgcca tcgagcgctc gctggtgcag cgctacggcc
      721 cgaagatcct cgccgtgccc cccggcagcc ggctgcccag cgtcggaatc gggctcggtt
      781 tggtcgtgat cggcgcgggc atcggtgcgc tcacgctgct caagcgatgg cagcgtcgcg
      841 ccacaccggc gcttgcggac gggccgaaac tcgagcccgg tgccgccgac gctgagctcg
      901 acgcgcgggt cgatgccgag ctatcacgcc tcgaccgcga atgaacaagc tcgagcttcc
      961 tatcatcacc gcccgcggct ccgcgcgtga gctgggccac gcccagggcg aggcgctccg
     1021 cgagtcgatc gcggcgttcg tcgaccagcg cctcagcgcg cttcgggcgt acctcgccga
     1081 gcgcaacgag agcgctcggt actccgagtt cgtcgcggtc ggtgagcgct gcttggccat
     1141 cgcgcgcacc ttcgacccgc tcggcaccga ggagcacgaa gccatcgcgg aggcagcgca
     1201 cgtggcgccc gaactgctct acgccgccac gaacatgacc gacgtgcgcg acgtgctcgt
     1261 gctgccttcg acccccgacc gcgaggggtg cacgagcctc ttggttcccg cggaaagggc
     1321 gcgaggaaag caactcttgg cggggcagac ctgggacttg aacccgactg atctcgactt
     1381 cgtcgtcgcg gtgcatcggc tgccgaacga aggcccggag acgtggtcgg tgacctgcgt
     1441 cggcgccctg agcctcgtcg gtatgaacga gctcggcgtc gcggtcggca ccaccaacat
     1501 caagacgcga gcgtcgcgtc cgggcgtcgg ttacctctcg attctgcaca aggcggtcac
     1561 ccgtcggagc tacgaagacg cggttgcggt cgtgcggagc gctccgcgcg ccgcggcgca
     1621 cacgtactgg gtggcgagcg ccgcgcaagc gctcgagctc gagtgcgatc cggagctcgt
     1681 cgtcgagcgg ccgctccttg ccgacgcggt ggtgcaaacg aaccattgcc aagctgcgcc
     1741 gctcgcggcg cgtgagggcg aggccccgag cgcatcgagt cagcgccggc tcgcacgcgc
     1801 gggggtcttg ttggctcgct ccgaccaaga cttcagcagc gtcgaagcgc tgttcgccga
     1861 ccgtgcggac ggcgtcgaca gcatcaatcg ctacagcgag gacgcgcagg gcacgaccac
     1921 gaacgcctgc ttcgtgtgct gccccgcgac acgcgagatc cgcgcttgcc gcgggccggc
     1981 agatcgcggc gagtggcgtt cgctcgcgtt cgagcggggc tgagcgacta cttccgtttc
     2041 agcgggattc gcggcttttt cttcgacttc ggctccgcct ccgcggcgtc gccgctgccg
     2101 tcggcgcctt ttcggaagag cgcgtcggcg gcctcggctg ccttcttggc tcgctcggtg
     2161 tcgccgcgca tttccatgag gaagcggctc gggatgctcg gccgtggctt gccccacttc
     2221 attcgcgcct tggcgaacgt aatcgtcagc gtctcctggg ctcgagtgac gcccacgtag
     2281 caaagcctcc gctcttcgtc gacgccgcgc ccctccatga ccgagcgttg gtgcggcaaa
     2341 atcccttctt cgagcccgac catgaagacg tgcggaaact cgagcccctt cgcgctgtgc
     2401 agcgtcatga gcgtcaccgc gtgccgctcc cgcttgtcgt cctcgccgaa gtcgcgatcc
     2461 gagagcgtcg cctcctcgag aaatccgagc agggtaggtg aggtctcgcg ctgctcgtag
     2521 agcgccgccg cgttgaccaa ctcctcgacc gcttgccagc gcgcctccgc ctcgccgggt
     2581 ccggtgtgca cgcgctcgag ctcggtgcgg tactcgatgg tctgcaagag ctcgcgcatc
     2641 agctccgaaa tcggcacctg gccgagcttc gcgcggaagc cgtcgatgag ctttctgaag
     2701 ccctcgatgc gagtgatcgt gctggtcggg agctcgagca cctcgagcgc gagcgggagc
     2761 gcattccaga gcggcttgcc ttcgctaacc gcgaactcga gcagcccttc gatggtcgac
     2821 gtgccgatgc cgcgcgcggg ggtgttgatg atgcgtagca gcgagacttc gtccgagggg
     2881 ttcgagagca cccgcaaata cgacaagatg tcgcgaattt ctcggcggtc
//
LOCUS       DASTKS010001108        10309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1736827, whole genome shotgun sequence.
ACCESSION   DASTKS010001108 DASTKS010000000
VERSION     DASTKS010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736827"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFQ35_23380"
     CDS             complement(<1..1452)
                     /locus_tag="VFQ35_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933675.1"
                     /translation="MPQPSAQGAPNVDGALGMARSGASLAVTPKDELLLVADEDNQVL
                     RVVPLPLGTGAVRDMALPGRPAQVATDGEHVWVTVRAPSMLWVGRISSGEVSELARVA
                     LPVDAWGLAISADGKRAIVTSAWAAQVSFIDLQRAALRWSRPVSREPRGVALTRDGST
                     AYLTHLVGSHVTRIGLDDARAAQLALPPAPLRARGRVLSASLGYAGVLSPDGARFFAA
                     RHALGAPGKNAWFGAATVDVLGLERPEPVLDTPPTKRPELKSELAQTLLSGADTALVG
                     QTLTPFTQPRAVVYRRSTNTLLVAGEGDDRIVELDALAPDPTLAALAAYRVGEEYHAE
                     IHVAARGAAPSGLALSSDEHKLWVYCRGSSDLAEVALRTPGEPPAPTRVTARLDLGED
                     PLGKGGSTGRKLFYAARDFPVSGGLGCAGCHPEGRDDGYVWHEATFTTEDGETTNFVG
                     IADNIPPEAHARGFARRTPMLAGRVNPVGPYGWH"
     gene            1736..2134
                     /locus_tag="VFQ35_23385"
     CDS             1736..2134
                     /locus_tag="VFQ35_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933676.1"
                     /translation="MPSLTNNVLAFCELLERGEPLRAMERFYADDVCVFDNRELARAG
                     REQCLAHEREALTRLPAPPSFKVHRYAVNDATQVVFIEYTLRFLSEAGRPMRIEQVSV
                     QTWEGARISTERFYYEGVVDEGDEPPRSAE"
     gene            2331..2669
                     /locus_tag="VFQ35_23390"
     CDS             2331..2669
                     /locus_tag="VFQ35_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933677.1"
                     /translation="MSELVLRFVIAGTLVSLFAAAASSFQPKTFAGLFGSAPPIALAS
                     LAVAFATDGPLAVEKLARSMFFGAVALFAYSAVTALLCRRIPVWLSAVLSLGVWGLVA
                     SICFVGLNRS"
     gene            2678..3079
                     /locus_tag="VFQ35_23395"
     CDS             2678..3079
                     /locus_tag="VFQ35_23395"
                     /inference="COORDINATES: protein motif:HMM:NF022789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HET9933678.1"
                     /translation="MERPVFSLAELKNTSGAQYAGRFVFGGMVAALAALITRQAGPVA
                     GGLFLAFPAMLPASLTLVSERDGRKCAYDDARGACLGSIGLAVFAAVVWSLATARVHA
                     SVPLLAALVAWMLTSGGLWWVALRREGASKR"
     gene            3142..4809
                     /locus_tag="VFQ35_23400"
     CDS             3142..4809
                     /locus_tag="VFQ35_23400"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9933679.1"
                     /translation="MLAASRPATTRRRDFFSSLRTLVELFAIATLVSACGAATLATQS
                     SREVEVPPYASAYSTDAGRSGVRGSEAATRAEHQLKEKLRQRGDGEAKPDAALAATAA
                     WALKRAYANQNLSDTRVLADAAMRFGFTGLVQGFAIMPLSDARAEELIGSMLAGVPGN
                     SRVTRYGIVGGAGSDVAFLIGAVEASLEDFPRAVAPGGSLRLSGELDPRFERASVFST
                     NPSGETREIAMPSRKVEATLEFPLVGTYSLELMGYGSTGPVVLMNVPIRVGVEEAAES
                     EGDVQVDPNLTPEKAQDELLSLLNTERVKHGLARVELDGELGAIALAHSEDMALHDFF
                     GHVSPNTGAPEDRVRKADVRVSRVGECVALELTPERAHRGLLASPAHRAAMLDPRFTH
                     VGIGVAFRGDEPPPRRLRVTLLLGRRVPPEETRQSAESVAAYVQSLRQSRKLGSVRVD
                     PILTAVASAGTTALANASDKSSRTALAASASELQRQVNRTKRSRSVCQSFVEVLEREE
                     LGSVGLLSDPDLASIGVGVVELTDAKGSRLGVLVAGEARPGSALRCR"
     gene            4857..5660
                     /locus_tag="VFQ35_23405"
     CDS             4857..5660
                     /locus_tag="VFQ35_23405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933680.1"
                     /translation="MRLGSLIGWLVLGACACSPREPAAPAARSVDTATMPPAVANGGV
                     PATPPPVPALPSAGASAAPPPAPAQAGEPSAWTDPAVVAALALDCNFKPAAPAPDEPE
                     PSPLSCASGLYEQSCIYDPCYSTNQGECAPRCQKACDACSEQCSAGCGRCKARCTDDA
                     CRHECATACASCKQECLATKDRCATGTCGAEYKACIEQLTKRWVASGCEKTCKAYRDC
                     TDKCQAGPACTSSEDEKACSTCTDRCAGQVKRGCPKEFVEMCLFNGGLP"
     gene            5657..6586
                     /locus_tag="VFQ35_23410"
     CDS             5657..6586
                     /locus_tag="VFQ35_23410"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9933681.1"
                     /translation="MRHGSLRAAAYAAITSLWTVTTPARAESSGVKAREGEMVHVPAG
                     SFVRGLRATRRDEGPPHRVVLSAFDIDATLVTRAAFTRFVEETGYVTSAERRGYGVAA
                     REGMDDWAWERVPHGSFRRPFLGEGTGDNAAFLRDDAPAVMISHSDATAFCAHYDARL
                     PTEAEWEYAMGAESNGTRYPWGDEPERADGKLGLNFWQGVSHHKNLRLDGFVYVSPVS
                     AFSPNARGIYDPVGNVWQWTADWYAPDTYARAGARVTNPTGPADGTRRVLRGGSWWCG
                     ACTCEGNGLSYRGKADPEAAFNNNGFRCVRPAR"
     gene            6742..7752
                     /locus_tag="VFQ35_23415"
     CDS             6742..7752
                     /locus_tag="VFQ35_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9933682.1"
                     /translation="MKVYELRGPGLDSLVQAERPEPQPSETQLVVRMRAASLNYRDLL
                     VATDRYGRGPIPFPLVPLSDGAGEVLRVGSRVTRFQPGERVLGNFFQKWEEGPFDSQK
                     AASALGGAIDGVLAEEVALEESGAAHVPNGLSFEEAATLPCAGLTAWVGLVRLGKLAA
                     TDTVLAMGTGGVSIFALQIGKAVGAKVILTSSHDEKLARGRELGADESINYRETTDWD
                     AQARALTGGDGVDQLLEVGGAATLPRSLAAVRPGGHLCLVGLLSGSTSDRGEAAKNQR
                     GVRVDSVYVGSTQDLREFCAFIEKYSLRPVIDRAFSFDEARGAYDYLQKQAHFGKVVI
                     RL"
     gene            complement(7806..8183)
                     /locus_tag="VFQ35_23420"
     CDS             complement(7806..8183)
                     /locus_tag="VFQ35_23420"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HET9933683.1"
                     /translation="MHIYVTARHFDLTPSIHSYVEKRLVEATQGHAASHDLLRMEVQL
                     TQLDAHDARFTCHILLQLRGHHDINITEETFDLYEAVDRAEKRLIRKLTDERQRQLTA
                     ARHAKLQDLEPLDDPSLLDQSSR"
     gene            8389..9699
                     /gene="nhaA"
                     /locus_tag="VFQ35_23425"
     CDS             8389..9699
                     /gene="nhaA"
                     /locus_tag="VFQ35_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET9933684.1"
                     /translation="MASGSEGRRPQWPSAPPGSWWLAHRLARRAIAPVERFLQVEAAS
                     GILLLLAAALALVWANSPWSSAYSALWHTPVGVHFGDFAFERDLHFWINDGVMTIFFF
                     VVGLEIRGEIHHGELSDARRAALPLFAALGGMLVPAAIFASFNAGHASLRGWGVPMAT
                     DIAFAVGVLTLLGSRVPATLRVLLLALAVIDDVGAILVIALFYSSELQPLGFAVLGAG
                     LGAILLQRAMGVTRPWLYVPAGILSWAGAYAAGIHPTLAGVAIGLLTPVQPWPNPDGD
                     GQSSPAQRIEHALHDWVAFGAMPLFAFANAGVSLGSAEFAGDSLLAFVGIFCGLVLGK
                     PIGVVAASWLATRLGIAVLPRGTSWKQIALVGVVAGIGFTMSIFIAGLAFGRGPTLET
                     GKIAILLGSFTAAVLTYGFGRAILPQRSSATRNEAVPELLGNSE"
     gene            9990..>10309
                     /locus_tag="VFQ35_23430"
     CDS             9990..>10309
                     /locus_tag="VFQ35_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933685.1"
                     /translation="MTSDRNRLFRRVGACLLISGGVAAFGCTSARDSQPKFIEGSGGQ
                     VATAQGGATAMASGGSGFGGAVIQVPTTPIPDDPNGCKALSCHPEGGDYCGEVGDGCN
                     GKMN"
BASE COUNT         1678 a         3394 c         3446 g         1791 t
ORIGIN      
        1 gtgccagcca tatggcccca ctgggttgac ccttcccgcg agcattggag tgcggcgagc
       61 aaagccgcgc gcgtgtgctt cgggcgggat gttgtcggct atgcccacga agttggtcgt
      121 ctccccgtct tcggtcgtga aggtcgcttc atgccagaca taaccatcgt cgcggccctc
      181 tggatgacag ccagcacaac caagccctcc gctgaccgga aagtctcgcg ccgcgtagaa
      241 gagctttcgc cccgtgctcc cacctttgcc gagcggatct tctccgagat ccaggcgcgc
      301 cgtcacccgg gtcggcgcgg gaggctcgcc cggtgtgcgc aacgcgacct cggccaagtc
      361 gctcgagccg cggcagtaca cccacagctt gtgttcgtcg ctcgacagcg cgagcccgct
      421 cggagccgcg ccgcgagccg ccacgtgaat ctcggcgtga tactcttcgc cgacgcggta
      481 agccgcgagc gcggccagcg tcgggtccgg cgcgagcgcg tcgagttcga cgatgcggtc
      541 gtcaccttcg cccgccacga gcagcgtatt cgtagagcga cggtacacga cggcgcgggg
      601 ttgcgtaaat ggggtgagag tctgcccgac gagcgcggta tcggcgccgc tcagcagggt
      661 ttgtgcgagc tccgatttga gctcgggacg cttggttggg ggggtgtcga gcactggctc
      721 gggccgctcg agtccgagca cgtcgaccgt agcggcaccg aaccaggcgt tttttcccgg
      781 cgcgccgagg gcgtggcgcg ccgcaaagaa gcgcgcgcca tcgggtgaaa gcactcccgc
      841 atacccgagc gaagcggaga gcacgcgtcc tcgggcacgc agcggcgcgg gaggcagcgc
      901 gagttgcgcg gcgcgcgcgt cgtcgagccc gatgcgcgtg acgtgcgacc cgacgaggtg
      961 cgtcaggtac gctgtgctgc cgtcgcgcgt cagcgcgaca ccgcgaggtt cacgcgatac
     1021 cggtcgcgac caacgaagcg cggcgcgttg caagtcgatg aacgacacct gagccgccca
     1081 cgcactcgta acgatcgcgc gcttgccgtc ggcgctgatg gcgagccccc acgcgtcgac
     1141 cggcaaggca acgcgagcga gctcgctcac ctccccgctc gaaattcttc cgacccaaag
     1201 catcgacggc gctcgaacgg tgacccagac gtgctcccca tcggttgcca cttgtgcggg
     1261 acgccccggc agcgccatgt cgcgaaccgc tcccgtgccg agcggcagcg gcaccacgcg
     1321 cagcacctga ttgtcctcgt ctgcaaccag aagcagctcg tctttcggcg ttacggccaa
     1381 gctcgcgccg ctgcgagcca tcccgagcgc cccgtcgacg ttcggcgcgc cttgcgcgct
     1441 cggctgcggc actgcactcg agggcgctcg tgcggtcgcg gaagcagagg ccgacggggc
     1501 cggcccttcc cgctcgcagc gacagccgcc aagaatgcta attgcggcaa acatcaccaa
     1561 agcacgcacc tgcaggccca cccgaccgac tccgacccga accatcgacg ccgaggctac
     1621 ctcactcgcg cgtctcgaat ccagcggtgc cgagacggac tgatgaacgc cgaacacagc
     1681 cggccgattc cgcgtcgcgc tggccgccag ggctcggctt ggctacgtac cgcccatgcc
     1741 gagcctgacg aacaacgtgc ttgccttttg cgagctcctc gagcgcggag agccacttcg
     1801 ggcgatggag cgcttctacg cggacgacgt gtgcgtgttc gacaatcgag agctcgcgcg
     1861 cgctggacgc gagcagtgcc tcgcgcacga gcgtgaagcg ctgacgcggc tccccgctcc
     1921 tcccagtttc aaggtgcatc gctacgccgt caacgatgcg acccaggtcg tgttcatcga
     1981 atacacgctc cgcttcctat ccgaggcggg gcgccccatg cgcatcgagc aagtgagtgt
     2041 ccaaacctgg gagggcgcgc ggatctcgac ggaacgcttc tactacgagg gagtcgtcga
     2101 cgaaggcgac gagcctccgc gaagcgccga gtaacgaact ctacgctgcg cccccgacag
     2161 agctgcctcg tgcggcgttc tcgccgcgca cactccgcgg cgaatacgga gcgggcatcc
     2221 catccgtccg actgcgcggc gagcgtgtcc gggtgcggct tggctcttgc actcgggtcc
     2281 gcagatgccc ggtcagcgtt tcgttgccgg cgaatggagg cgcctcgccc gtgtcggaac
     2341 tcgtgctcag attcgtcatc gcgggaacgc tcgtgtcgct ctttgcggcg gcggcgagca
     2401 gctttcagcc gaagactttt gctggattgt tcggctcggc gccgcccatc gcgctcgcta
     2461 gcttggcggt cgcgttcgca acggacggtc cgctcgcggt ggagaagctc gcgcgctcga
     2521 tgttcttcgg cgcggtagcg ctctttgcat acagcgcggt tacggcgctg ctgtgtcgcc
     2581 gcattcccgt ttggctgagc gcggttctgt ctctgggcgt ttggggtctc gtcgcgagca
     2641 tctgtttcgt cggcttgaat cgaagttgag gactcacatg gaaagacccg tcttctctct
     2701 cgcggagctc aagaacacga gcggcgccca atacgcgggc cgcttcgtct tcggcggcat
     2761 ggtcgccgcg ttggctgcac tcatcacccg acaggcggga ccggtcgccg gcggtctctt
     2821 cctcgcgttt ccggcgatgc tgcccgcgag tctcacgctc gtgagcgaac gggacggaag
     2881 gaagtgcgcc tatgacgacg cgcgcggcgc gtgcttgggc agcatcgggc ttgccgtctt
     2941 cgcggccgtg gtttggtcgt tggcgaccgc gcgcgtgcac gcctccgtac cgttgctcgc
     3001 agctttggtc gcgtggatgc tgacgtcggg tgggctctgg tgggtagccc tccgccggga
     3061 aggggcgtcc aagcgctgat acgcgagccg ccacgagcag ttgctcaggg ccgtgcggga
     3121 aggcaaggtg tgcacctcgc aatgctcgcc gcctcccgtc cagcaacgac ccgtcgccgc
     3181 gacttctttt cgagcctgcg cacgctcgtg gagctgtttg cgatcgcgac gctcgtcagc
     3241 gcatgcggcg ccgctactct cgcgacgcaa tcgagccgcg aggtggaggt tccgccttat
     3301 gcgagcgcat actcgaccga tgcaggccga agcggcgttc gcggcagcga agccgccacc
     3361 agggccgaac accagctcaa agaaaagctt cgccagcgcg gcgacggtga agcgaaaccc
     3421 gacgccgcgc tcgccgcgac cgcagcctgg gcgctgaagc gcgcctacgc gaatcagaac
     3481 ctctccgata cgcgcgtctt ggccgatgcc gcgatgcgct tcggcttcac cgggctcgtt
     3541 caagggttcg cgatcatgcc cctgtccgac gcccgcgcgg aggaactcat cggttccatg
     3601 ctcgcggggg tgcccggaaa cagccgtgtc actcgttacg gaatcgtcgg gggcgccggc
     3661 tccgacgtgg cgtttctgat tggcgcggtg gaggcgtcgc tcgaggactt cccgcgcgcg
     3721 gtcgctcccg gcggcagctt gcgcctgagc ggcgagctcg acccgcgctt cgaacgcgcc
     3781 agcgtgttct cgacgaaccc gagcggcgag acgcgagaaa tcgccatgcc ctcacgaaag
     3841 gtcgaggcaa cgctcgagtt ccctctcgtg gggacctatt cgctcgagct catgggctac
     3901 ggctcgaccg gccccgtcgt cttgatgaac gtgccgattc gcgtcggcgt ggaggaagcg
     3961 gccgagagcg aaggcgacgt ccaagtagac ccgaacctca cgcccgaaaa ggcccaggac
     4021 gagctccttt ccctcctcaa caccgagcgc gtcaagcacg gcctcgctcg ggtcgagctc
     4081 gacggtgagc tcggcgccat cgcgctcgcg cactccgaag acatggctct gcacgacttc
     4141 ttcggacacg tctcgccgaa tacgggcgcg cccgaggatc gcgtgcgcaa ggcggacgtg
     4201 cgagtctcga gagtcggcga gtgtgtcgcc ctcgagctca ctcccgagcg tgcacaccgt
     4261 gggctcttgg cgagccccgc ccatcgcgcg gccatgctcg atccgcgctt cacccacgtt
     4321 gggattggcg tcgctttccg cggtgatgag ccgccgccga ggcgactgcg agtgacgctg
     4381 ctactcgggc gacgcgttcc gcccgaagag acgcgtcaaa gcgccgaatc cgtggccgcg
     4441 tacgtgcagt ctctgcgcca aagccgaaag ctcggttcgg tccgcgtcga tccgatcctc
     4501 accgcagtcg cgagcgccgg aaccaccgcc ctcgcaaacg caagcgacaa gagctcgcgg
     4561 accgcgcttg ctgcgagcgc gagtgaactt cagcgacagg tcaaccgcac gaagaggagt
     4621 cgaagcgtgt gtcagagctt cgtcgaggtg ctcgaacgcg aagagctcgg ctcggtcggg
     4681 ttgctgagcg atcctgacct cgcgtccatt ggcgtgggtg tcgtagagct gacggatgcg
     4741 aaggggtctc ggctcggagt gctggtcgcc ggcgaagctc gcccggggag cgctctccgc
     4801 tgccggtgat cggcgccgga cctcgcaacg actgaaagct ttggtagagt gacccaatgc
     4861 ggctcggctc gctcattggg tggctcgttc tcggcgcttg cgcttgctct ccacgcgagc
     4921 cagcagcacc cgcggcgcgc tccgtagaca ccgcgacgat gccgccagcc gtcgcaaacg
     4981 gcggcgtccc agcaactcct ccccctgtac ccgctctgcc gagtgccgga gcgtcggccg
     5041 cgccgccgcc cgcgccagcc caagccggcg agccatcggc ctggaccgac cccgcggtgg
     5101 tggccgcgct tgcgctggac tgcaacttca agccggccgc accagctccg gacgaaccgg
     5161 aaccatcccc gctctcgtgc gctagcgggc tctacgagca atcgtgcatc tacgacccct
     5221 gctactcgac gaatcaaggt gagtgcgcgc cgcgctgcca aaaagcgtgc gacgcctgca
     5281 gcgagcaatg ttcggccggc tgcggacgct gcaaggcacg ctgtacggac gacgcttgcc
     5341 gacacgagtg tgcgaccgcg tgcgcgagct gcaagcagga gtgccttgcg accaaagatc
     5401 gctgcgccac cggcacctgc ggcgccgaat acaaggcatg catcgaacaa ctcaccaagc
     5461 gctgggtcgc gagcggttgc gagaagacgt gcaaagcata ccgagactgt accgacaagt
     5521 gccaggcggg cccggcgtgc acgtcgagcg aagacgagaa ggcgtgctcc acctgcaccg
     5581 accgctgtgc gggacaagtg aagcgcggct gcccgaagga attcgtcgag atgtgcctct
     5641 tcaacggagg actgccgtga gacatggcag ccttcgcgct gcagcgtacg ccgcgatcac
     5701 gtcgctttgg acagttacga ccccggctcg ggccgagtcg tcgggagtga aggcgcgcga
     5761 aggtgaaatg gttcacgtcc ccgccggctc gttcgtgcgc gggttgcgag ctacccgccg
     5821 cgacgagggg ccgccgcacc gggtcgtgct gagcgctttc gacatcgacg cgacgctcgt
     5881 cacgcgcgcg gcgttcacgc gcttcgtcga agagaccggc tacgtcacga gcgccgagcg
     5941 gcgcggctac ggcgtcgccg cgcgggaagg catggacgac tgggcatggg agcgcgtccc
     6001 ccacggctcg tttcggagac catttctggg cgaaggcacg ggcgacaacg ccgctttttt
     6061 gcgcgacgac gcgcctgccg tgatgatcag tcacagcgac gcgaccgcct tttgcgccca
     6121 ctacgacgcg cgtctgccca ccgaagcgga gtgggagtac gcaatgggcg cggagagtaa
     6181 cgggactcgc tacccttggg gcgacgagcc agagcgcgca gacgggaagc tcgggttgaa
     6241 cttctggcaa ggcgtctcgc accataaaaa cctcaggctg gacggtttcg tctacgtctc
     6301 ccccgtgagc gcgttctctc ccaacgcgcg cggcatctac gatccggtcg gcaacgtctg
     6361 gcaatggacc gccgattggt atgctcccga tacttacgcg cgcgccggtg cacgagtaac
     6421 gaatccaacg ggtccagccg atggtacgcg acgcgtattg cgtggcggct cttggtggtg
     6481 cggcgcgtgc acgtgcgaag ggaacgggct cagttatcgc ggcaaggcgg atcccgaagc
     6541 agccttcaac aacaacgggt tccgctgcgt acgaccggca cgctgaacgc cgcgctgccg
     6601 cagtgaggcg caccggccgc gtacggcgtc atccgccatc cgcttcgttc gctagagatc
     6661 cgaacgatcg gggaggggca cgaccggtgc atggggtccc gtcgacccga gctcgtttgg
     6721 gccgtggtga aggatccccc catgaaagtc tatgaactac gcggacccgg actcgattcg
     6781 ctggtccaag cggagagacc cgagccgcaa ccaagcgaaa cgcagctagt cgtccggatg
     6841 cgcgcggcta gcttgaacta ccgcgatctc ctggttgcga cggatcgcta cgggcgcggc
     6901 cccatcccct tccctttggt tccgctctcg gatggcgcgg gtgaagtact tcgcgtgggc
     6961 tctcgggtga cgcggttcca gcctggcgag cgggtcctcg gcaacttctt tcaaaagtgg
     7021 gaagaagggc cgttcgactc gcaaaaggcc gcttccgcgc tcggtggagc gatcgacgga
     7081 gttctcgccg aagaagtcgc gctcgaagag tcgggggcgg cgcacgtccc gaatggcctc
     7141 tctttcgagg aagcggcgac gttgccgtgc gcagggctca ccgcctgggt cggcttggtg
     7201 aggctcggca agctcgccgc gaccgatacc gtgctcgcga tgggaaccgg cggcgtcagc
     7261 atcttcgctc tccaaatcgg caaggccgtc ggcgcaaagg tcattttgac gtcgagccac
     7321 gacgaaaagc tcgcgcgcgg tcgtgagctc ggcgcggacg agtccataaa ctatcgagaa
     7381 acgaccgatt gggacgccca agcacgagcc ctcaccggag gcgacggcgt cgaccaactc
     7441 ctcgaggtcg gcggcgcggc cacgttaccc aggtcactcg ctgcggtgcg tcccgggggt
     7501 cacctttgtc tggttggcct gctgagcggc tccacctcgg accgagggga ggcagcaaag
     7561 aaccaaaggg gggtccgggt cgattcggtt tatgtcggta gcacgcaaga tctgcgcgag
     7621 ttttgtgcgt ttatcgaaaa atattcgctg cggccagtca tcgatcgggc gttttctttc
     7681 gacgaggcgc gcggcgctta cgattacctt cagaagcaag cccactttgg caaggtcgtg
     7741 attcgcctgt aggcacgtcc agaaagtccg tcgagcgccg cgccatcctc ctctagcggt
     7801 cagcgttagc gagagctctg gtcgagcaag ctaggatcat cgagcggctc gaggtcctgc
     7861 agtttcgcgt ggcgcgcagc cgtgagctgc ctctgccgct cgtcggtgag cttgcggatc
     7921 agccgctttt ctgcgcggtc gactgcttcg tataggtcga acgtctcctc cgtgatgttg
     7981 atgtcgtgat ggccgcggag ctgcaacaag atgtggcacg tgaagcgagc gtcgtgcgcg
     8041 tcgagctgag ttagctggac ttccatgcgc aataggtcgt gggacgcggc atgcccttgc
     8101 gtagcttcga cgagtcgttt ctcgacgtag ctgtggatgc tgggcgtcaa atcgaaatgg
     8161 cgagcggtaa cgtagatgtg catgagcaga gctcctgtct tcgtgcctgc agccttcgtg
     8221 ccgagaactg catgggtgag gcggttcggc gcggagccga acgttcttgc gagacagcaa
     8281 tcgatccggt accttaattc cctcgggtgc agcggccagt cgctgcagga acttggttgg
     8341 tcgggccgcc agccactagc aaccagcaga gctggtgagg tctcccgtat ggctagtgga
     8401 agcgaaggtc gacgcccgca atggccgagc gcgcctccgg gcagttggtg gctagcgcat
     8461 cggttggccc gacgcgccat tgctcccgtc gagcgctttc tacaagtcga ggccgcgagc
     8521 ggaatcttgc tcttgttggc ggccgcgctc gcgctcgtct gggccaactc gccgtggagt
     8581 agcgcgtact cggcgctgtg gcacacgccg gttggggtcc acttcggcga cttcgcgttc
     8641 gagcgcgact tgcacttttg gatcaacgac ggcgtgatga cgatcttctt cttcgtcgtc
     8701 gggctcgaga tccgcggaga gatccaccac ggggaactct cggacgcgcg ccgagcagcg
     8761 ctgcccctct tcgctgcgct cgggggaatg ctggtgcccg ctgcgatctt cgcttcattc
     8821 aacgccgggc acgcgtcgct tcgcgggtgg ggcgtcccga tggccaccga catcgccttc
     8881 gcggtcgggg tactcacatt gctcggaagc cgcgttcccg cgacgctgcg agtgctgctg
     8941 ctcgcgctcg ccgtcatcga cgacgtgggc gccatcctcg tcatcgcgct cttttactcg
     9001 tcagagctgc aaccacttgg tttcgccgtg ctgggagcgg gcctcggagc gatcttgttg
     9061 cagcgagcga tgggggttac gagaccctgg ctctacgtgc ccgctggaat cttgagctgg
     9121 gctggcgcct atgcagcggg catccaccca acactcgccg gcgtcgcgat cggccttctg
     9181 actccggtgc agccgtggcc aaaccccgac ggtgacggac agtcgtctcc cgcgcagcga
     9241 atcgagcatg cgctccacga ttgggttgcg ttcggcgcca tgccgctctt cgctttcgcg
     9301 aacgcggggg tgtcgctcgg gtcggccgag ttcgccggcg actcgctgct cgcgttcgtc
     9361 gggatcttct gcggtctcgt gctcggaaag ccgatcggtg tcgtcgccgc ctcgtggcta
     9421 gcgacgcgcc tcggcatcgc ggtcttacca cgcggaacga gctggaagca gattgcgctc
     9481 gtaggcgtcg tggcgggcat cggtttcacg atgtcgatct tcatcgctgg actcgcgttc
     9541 gggcgtggtc cgacgctgga aaccggcaag atcgcgatcc tgctcggctc gttcacggcc
     9601 gcggtcctca cctacgggtt cggaagagcg attttgcccc agcgttcgag cgcgactcgg
     9661 aacgaggccg tgccggagct cctcggcaac tccgaataac caacgcgctc ggcgcgacga
     9721 tcggacgtac gttccaagag cgcgcgctga ctggaacatt cgcagaccat gcgaagagtc
     9781 acgcgcgtcg tcgcgaattg ccacgtgctc gtcataaaag gccggcggaa aaaggcgcgc
     9841 gcccaacgat ttctgcgccg cacacgtgtg atgtcggcac tatcgaagaa cgacggttcg
     9901 ggcgaagtgc tcgccatctc cgagctcctg gcaccgcccg cgcaaggcaa cgctgcaacg
     9961 ttgtctcggt cgagccgcga ggttctttca tgacgtcaga tcgaaatcga ttgttccgcc
    10021 gcgtcggcgc gtgcctgctg atttccggtg gagtcgcggc gttcggctgt acctcggcgc
    10081 gcgattcgca acccaagttc atcgaggggt ccggtggcca ggtagcgact gcgcaaggcg
    10141 gggcgaccgc catggcgtcc ggtgggagcg gctttggtgg cgcggtgatt caagtgccga
    10201 ccacaccgat tcccgacgac ccgaacggct gcaaggcgct ctcgtgtcac cccgagggcg
    10261 gcgactactg cggcgaagtg ggcgacggat gcaacggaaa gatgaactg
//
LOCUS       DASTKS010001109         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_619777, whole genome shotgun sequence.
ACCESSION   DASTKS010001109 DASTKS010000000
VERSION     DASTKS010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619777"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..839)
                     /locus_tag="VFQ35_23435"
     CDS             complement(138..839)
                     /locus_tag="VFQ35_23435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9933686.1"
                     /translation="MPCSKMHGGWGEEAQNIIVTRLPTKRPLLQVVPTTPEAPDALSD
                     EELLEGLARGDAGIGSELCRKLMRVVDGTLYRVLGGRDVDHEDLVQASFEQIVRSLSK
                     GKFARECSLSTWAAAITCNVALHAIRRRRTERKIFDDAFGFEDLTHKPASGSDPESQV
                     NSRRELERVRRHLGQMSEKLAQALLLSDVLGCTQAEVASLLDVSVPAAQARLFRGRQE
                     LALRLTRETAGGKAR"
     gene            complement(875..1909)
                     /locus_tag="VFQ35_23440"
     CDS             complement(875..1909)
                     /locus_tag="VFQ35_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933687.1"
                     /translation="MPGWFVAVELPTRDTTQQESAALISACSAALGRGKCALSEAAES
                     APAVAVVSFRGAERLRALVEVGRRETESRSWRSQEIAFRPEDARLERFRTLGLAIATL
                     VRESKIEPVPPPEGVASFPEEKKPQDIPKETPPTPPPKAAPEKPLAREVSRTEVARPA
                     PAPPLPTKREPPVWLSVGALGAYDHELPSSMRYGGRIGVGVTRVGLPLYASLTGAYSV
                     GAAPGDEQAVSRVSLAWATIAAGFGARMRLPFDTEGRAAIQGVLVDLTAHAAASGDVE
                     EEGQRWILGGQLELELAALRSRTFGFTLGGAAQHLTGATALVVHRDRVATSAAFTWSV
                     HAALELHLFR"
     gene            2016..2549
                     /locus_tag="VFQ35_23445"
     CDS             2016..2549
                     /locus_tag="VFQ35_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933688.1"
                     /translation="MRKRPITLRHLTIIIPLLFSAACSGSGGDPAQGSGGAGGTSGDP
                     LNAPPTCTSKMTWTRGENAQMRPGEACISCHTQDGGPRFSIAGTVYPTGHEPDDCNGA
                     AGATIVIVDAQNREHRITANAVGNFYLEQTIAVPYTAHVIGTNGERFMVSPQTSGDCN
                     GCHTEWGHGAPGRINIP"
BASE COUNT          473 a          882 c          804 g          426 t
ORIGIN      
        1 gagatcaccg agcaggcgct ccacgtgcgc cgcgctgcgt tgaaccatcg cctcttcttc
       61 tgctggcgac ggcaacggca cgaccaatcg tcccatttgt ccgagcaggc gccgggcagc
      121 tcgcgcatcc aagcgagtca tcgcgccttc cctcctgcag tctcccgagt gagacgcaaa
      181 gcgagctctt ggcggccgcg aaacaagcgt gcctgggccg ccggcaccga gacgtcgagc
      241 agcgaagcaa cctccgcttg cgtacaaccg agcacgtcgc tcaagagcag cgcctgcgcg
      301 agcttctcgg acatttgacc gaggtggcgc cgcacacgct cgagctcgcg gcgtgagttc
      361 acctggctct cggggtccga cccgctcgcg ggcttgtgag tgagatcctc gaagccgaac
      421 gcgtcgtcga agattttgcg ctccgtgcgg cgacgccgaa tcgcgtgcag ggcgacgttg
      481 caggtgatcg cggccgccca cgtcgagagg ctgcactcgc gggcgaactt ccctttgctg
      541 agcgagcgca cgatctgctc gaacgaagcc tggacgaggt cttcgtggtc gacgtcacga
      601 ccgccgagca cccggtagag cgttccgtcg acgactcgca tcagctttcg gcagagttca
      661 ctaccgatgc cggcgtcgcc tcgggcaagc ccttcgagca gctcctcgtc gctgagcgcg
      721 tcgggagcct ccggagtcgt cggaacgact tgaaggagtg gacgcttcgt cgggagcctc
      781 gtcacgatga tgttctgtgc ctcctcgccc cagccgccat gcattttgct gcacggcaca
      841 ttaaccgccg cctaacagat cgacaattcc gccattaacg gaacaaatgt agctcgagtg
      901 cggcatgaac cgaccacgtg aaagcggcac tggtcgctac tcgatctcga tgcacgacga
      961 gcgccgtagc ccccgttagg tgctgcgcag cgcccccgag agtgaaccca aaggtgcggg
     1021 aacgcagcgc cgccaactcg agctccagct gcccgcccaa gatccaacgc tgcccttctt
     1081 cttcgacgtc gccagaggcg gctgcgtgcg ccgtcaagtc gacgagcaca ccctgaatcg
     1141 cggcgcggcc ctcggtatcg aaaggaagcc gcatgcgagc gccgaagcct gcggcgatcg
     1201 tcgcccaagc caacgagacg cgcgacacgg cttgttcgtc tccaggcgcg gcgccgacgc
     1261 tgtacgcccc agtcaggctc gcgtacagcg gcagcccgac gcgtgtgacg ccgacaccaa
     1321 tgcgtccgcc gtatcgcatc gaagacggta gctcgtggtc ataagcaccg agtgcaccta
     1381 cgctgagcca cacggggggc tcacgcttgg tcggcagtgg cggagcgggg gcgggacgcg
     1441 cgacctcggt tcgactcact tcgcgcgcca aaggtttctc gggcgcggcc ttcggcggcg
     1501 gggtaggggg cgtttctttt ggtatgtctt gcggcttctt ctcttctgga aatgaggcca
     1561 ctccctcggg cggcggcacc ggttctatct tcgactctcg tacgagcgtc gcaatcgcaa
     1621 gacccagagt gcgaaaacgt tcgaggcgcg cgtcttcagg cctgaatgcg atttcctgcg
     1681 accgccacga gcgcgattcg gtctcgcgcc ggccgacctc gacgagtgcc cgcagcctct
     1741 ccgccccgcg gaatgacacc acggcgacgg ccggagcaga ctccgccgcc tcggacagcg
     1801 cacactttcc tcgaccgagc gcggcggagc aggctgaaat cagcgccgcg ctttcttgct
     1861 gcgtcgtgtc tctggtcggc agttcgaccg ccacgaacca tccgggcatc gcgtgaatgc
     1921 ttatgcatca acgcgcccaa gtcgactagt tggcgcctga gcccgggtcg cgcgcgcaaa
     1981 aacgcgctag tctacttgga cgagtgcgtt cctccatgag aaagcgcccc atcacgctgc
     2041 gacacctgac gattatcatt ccccttctct tcagcgctgc gtgcagcgga agcgggggcg
     2101 atccggctca aggctcgggc ggcgccggcg gcacttcggg tgatccgctc aatgcgcctc
     2161 cgacctgcac gagcaagatg acgtggacgc gcggagaaaa tgcgcagatg cgccctggag
     2221 aagcgtgcat cagctgccac acgcaagatg gaggaccgcg cttttcgatc gcaggcaccg
     2281 tctacccgac aggccacgag cccgacgact gcaacggcgc ggcgggagcg acgatcgtca
     2341 tcgtcgacgc gcagaaccgc gaacatcgca tcaccgcaaa cgcggttgga aacttctatc
     2401 tcgaacagac gatcgccgtt ccgtacacgg cccacgtcat cgggacgaac ggggagcggt
     2461 tcatggtgtc cccgcaaacg agcggcgact gcaacgggtg ccacaccgag tggggacacg
     2521 gcgccccagg acggatcaac atcccatgag acccatcgtt cttcgcgcac acccaacgtt
     2581 gctcc
//
LOCUS       DASTKS010001110         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_619884, whole genome shotgun sequence.
ACCESSION   DASTKS010001110 DASTKS010000000
VERSION     DASTKS010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619884"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFQ35_23450"
     CDS             complement(<1..703)
                     /locus_tag="VFQ35_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933689.1"
                     /translation="MKVRSNCLVAAFVLAACGPSGTSSTGVGNPSASFSLSIVNDEEE
                     AESGGTANLGGAGESNAGAPNDAGAPGVPADEVPLARGQIKNALLSIGELRFLPCVSG
                     VPVEVVTMPFIVDLVEKRTYPALPKIRDVEGGYCGIEARLAPARRDPSLVGRSLFFSG
                     TRADGTRFLLYADMQAKLRLRAPAGLPMAGPTPNLVWAMRPRRWLTKAEVDSADTEMV
                     EGLRTIVIDVDRHPLL"
     gene            complement(687..1577)
                     /locus_tag="VFQ35_23455"
     CDS             complement(687..1577)
                     /locus_tag="VFQ35_23455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HET9933690.1"
                     /translation="MKTRRAPKPTKDVGVSVYDFLDYRAYLRAYYEAAKRARPSFSFR
                     FFSKLAGLKSPNFLKLVMDGERNLGAESVARFASALGLAPREAEFFADLVAFNQAENM
                     AEKNRSFERIAASRRFRQARRIDGDLFAYLSRWYNPAVRELAAREDFQEDPRWISAQL
                     LPRITPVEAAEAMKLLLSLGLLVRDPVTARVVRGEPTLTTEHEVRSLGAAAFHRQMLE
                     RAAQSLDLVPGNKRDLAALTVCVSAETAAVVKQRIHEFREALTELCDSDTRGAVVYQL
                     NVQWFPLSSFGEEGHREGSK"
     gene            1798..2328
                     /locus_tag="VFQ35_23460"
     CDS             1798..2328
                     /locus_tag="VFQ35_23460"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9933691.1"
                     /translation="MRRGAVVGVLVETASIADAAEIARVHVETWRAAYRGIMSSEFLA
                     GLDVATREEQWRNWIELPERRTLIAKQGGALLGFCLTGPARDPDANDATFEIYALNVG
                     EAHWRSGCGRALLERAIDDAADDGAAQLTLWVLEQNQRARLFYEKLGFSPDGVSKIVQ
                     MGGVPLNELRYRRKLA"
     gene            2408..2806
                     /locus_tag="VFQ35_23465"
     CDS             2408..2806
                     /locus_tag="VFQ35_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933692.1"
                     /translation="MTCRSIIRSTVSGLLLLYVVGCSSDGSRGTEADRRGVGASCAQP
                     TDCTEAGQSCLPFKGGYCGIADCTRDADCPTGSACVQHDDGRNYCFLVCVEKVDCNVN
                     RPAELESNCSSNVTFVSGNKAIKACVPPSG"
     gene            complement(2778..3608)
                     /locus_tag="VFQ35_23470"
     CDS             complement(2778..3608)
                     /locus_tag="VFQ35_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933693.1"
                     /translation="MSQSLTRLFRAHAVVRLVLHIVVLFLPVRIALAQTPPLTLPETE
                     EEPGVVVYPARADGPRPVTVVLHGMCGEPERTCRYFSALVSEHEHLICPRARRRCDGG
                     GSIWPEAASEQIEAAVVRGIRALGPLVDESAGRTLIGYSMGAFRALTVAETSQGRYPR
                     VMLIGARIYPSQRLLLQNGVSRLLLAAGDWDMMSAHMQQQTKRLIASGYRARFMSLGR
                     VGHAFDQSFPEYLATALTWLHRPSAEALLTNSSGAYSSAPRRQRILVASLNRTGEHKP
                     "
     gene            complement(3857..4897)
                     /locus_tag="VFQ35_23475"
     CDS             complement(3857..4897)
                     /locus_tag="VFQ35_23475"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9933694.1"
                     /translation="MAVRVNLEAAELNRRLADPNLFREVQAAVRRRIQSDEALDVVQA
                     TFAEVLAARDVPADEEEFRRFVFGVARHKVFDHFRKRAREVPSDDANADEQAPEPLSA
                     RDLLRWAERELPDSESQHTLEWMLREGDGEKLEHIARDADVPAPRVRKRVSRLRSFLR
                     QRWATELLLAGVVLSLGVYLYSRHRDEAEHANPEVAREDAPLELARKLRRAALERCRD
                     GAVDECIAGLDRARSLDPAGDSAPEVAEARRRATSRPLPSPSTDAPTPVPSSDAPRPL
                     PSSTERVPVEHGKGTSSDFSAPVSPRRHTSKVATPPAPDSDSSLAPRGIDLRGSKDES
                     LPTPRRRLGDSK"
     gene            complement(5065..>5505)
                     /locus_tag="VFQ35_23480"
     CDS             complement(5065..>5505)
                     /locus_tag="VFQ35_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146724548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933695.1"
                     /translation="SVGDGCGEINVFEVVMDNNDYSNREFASTGVRSFQAGHVGGSVC
                     KSSCDRNAFPTDVEIVDACAKKAYDKGPVVVAGGASDGCPVWRRPKGDRYFFILLDET
                     SRTIQVGVVHPSNIPAALAPLLPELPSSLSRGAVDGVVNLRLPG"
BASE COUNT          975 a         1744 c         1836 g          950 t
ORIGIN      
        1 agagcaacgg gtgacggtcg acgtcgatca cgatggtgcg gagcccctcg accatctccg
       61 tgtccgccga gtcgacttcg gctttggtca gccagcgccg cggccgcatc gcccagacga
      121 ggtttggggt cgggccggcc atgggaagcc ccgccggcgc gcgaagccgc aacttggctt
      181 gcatgtctgc gtagagcaaa aagcgcgttc cgtcagcgcg tgttccgctg aagaacagcg
      241 agcgaccaac caaactcggg tcacgacgag ccggcgcgag gcgagcctcg atgccgcagt
      301 agccgccctc tacatcgcgg atcttcggca acgccgggta agtgcgcttc tcgacgaggt
      361 cgacgatgaa cggcatcgtt acgacctcga ccggtacgcc cgacacgcaa gggaggaagc
      421 gcagctcacc aatcgacagg agtgcattct tgatctgccc acgggcgagg ggcacttcgt
      481 ctgctggtac gcccggcgcg ccggcgtcgt tgggggcgcc cgcgttcgac tcccctgcgc
      541 cgcccaggtt cgcggtgccg ccgctctcgg cctcctcttc gtcattgacg atggagagcg
      601 agaagctcgc gctcggattg ccgacgccag tcgagctcgt gcccgatggg ccgcacgccg
      661 cgagcacgaa ggcggcgacg aggcagttac ttcgaacctt cacgatgacc ttcctccccg
      721 aatgacgaaa gcgggaacca ctggacattg agttgataga cgacggcgcc gcgcgtgtcc
      781 gagtcgcaaa gctcggtcag cgcctcccga aactcgtgga tgcgctgttt cactacggcc
      841 gccgtttcgg cgctgacgca cacggtgagc gccgccaaat cgcgcttgtt gcccggcacg
      901 agatcgagcg actgcgcggc tcgttccagc atttgtcggt ggaatgcggc ggcgccgagc
      961 gagcgcactt cgtgctcggt cgtgagcgtg ggctcgccgc gcacgacccg agccgtgacc
     1021 gggtctcgga ccaatagccc cagcgaaagc agcagcttca tcgcttcggc cgcctcgacc
     1081 ggcgtgatcc gcggcaggag ctgcgccgaa atccagcgag gatcctcctg gaagtcttcg
     1141 cgcgcggcga gctcccggac cgccgggttg taccagcgag acaagtaagc aaagagatcc
     1201 ccgtcgatgc ggcgcgcctg gcgaaagcga cgagaggccg cgatgcgctc gaacgagcgg
     1261 ttcttctcgg ccatgttctc ggcctggttg aaggcgacga gatccgcgaa gaactcggct
     1321 tcgcgcggtg cgagcccgag cgcgctggca aagcgagcca ccgactccgc gccgaggttg
     1381 cgctcgccat ccatcacgag cttcaagaag ttcggcgact tcaagcccgc gagcttcgaa
     1441 aagaagcgaa aggagaagct cggccgcgcg cgcttcgcgg cctcgtagta agcgcgtaag
     1501 tacgctcggt agtcgaggaa gtcgtagacg ctgacgccga cgtccttcgt aggtttggga
     1561 gctcgccgcg tcttcattcg atagacagtc tgcggcagcc acgaacttgt ttcaatgcgc
     1621 cctggaccgg ttcagtatac ggcctggata cgcagatggc tgaaacgggc ttattggtgc
     1681 gtattcgaga tgaatacaca aaagtatctg aatacacctg gggccttgtc gggcagtctc
     1741 gggtcggact tttcgggagg acggggcatg gaaagcgggt cgatgaacgc aaatgtaatg
     1801 agacgaggag ccgtggtcgg agttctggtg gagacggcgt ctatcgccga cgcagccgag
     1861 atcgcgcgag tgcacgtcga gacttggcgc gcggcttacc gcggcatcat gagctcggag
     1921 ttcttggcgg gtctcgacgt agccactcgc gaagagcaat ggcggaactg gatcgagttg
     1981 cccgagcgtc gcaccctgat cgcaaagcag ggcggagcat tgctcggctt ctgtctcacc
     2041 gggccagcgc gcgatccgga cgccaacgac gccacgttcg agatctacgc cctcaacgtc
     2101 ggagaggcgc actggcgctc tgggtgcggt cgtgcgctgc tcgagcgtgc catcgacgac
     2161 gcggccgacg acggtgccgc gcagctcacg ctttgggtgc tcgagcagaa ccagcgcgcg
     2221 cggctcttct acgaaaagct cggcttctcg cccgacggcg tgtccaaaat cgtgcagatg
     2281 ggtggagtcc cgctgaacga actgcgttac cgccggaaac tcgcctgagc ccgatgtgat
     2341 ctcgggtgtg gcgacccctc acccgcgtat tcataaaata cggcgcgcgc ccccactcac
     2401 gggcgagatg acctgccgtt ccatcattcg aagcacagtc tcgggcctgc tcttgctgta
     2461 cgtcgtcggt tgcagcagcg atggctcccg cggcacagag gctgaccgtc gtggcgtcgg
     2521 cgcgagctgc gcgcagccga ccgactgcac cgaggccgga cagagctgtc tgcctttcaa
     2581 aggcggttat tgcggcatcg ccgactgcac ccgagacgcc gactgtccca ccggttcggc
     2641 gtgtgttcaa cacgatgacg gccgtaacta ctgcttcctc gtttgcgtcg aaaaggtcga
     2701 ctgcaacgtc aaccggcccg ctgagctcga gtcgaactgt tcttccaacg tgacgttcgt
     2761 gagcgggaac aaggctatca aggcttgtgt tcccccgtcc ggttgagtga agcaacgagt
     2821 attcgttggc ggcgcggcgc tgacgagtat gctccagacg aattcgtcaa aagagcttcg
     2881 gcggacggtc gatgcagcca cgtgagtgct gtcgccaaat attcgggaaa gctctggtcg
     2941 aaagcgtggc cgacgcgccc gaggctcatg aatcgagcgc ggtagccaga tgcgatcaat
     3001 cgcttggtct gctgctgcat gtgcgcgctc atcatgtccc agtcgcctgc ggcgaggagc
     3061 agacgtgaga cgccgttctg cagcaaaagg cgctggctcg ggtagatgcg agcgccgatc
     3121 aacatgacgc gtgggtagcg accttggctc gtctcggcca ccgtgagcgc gcgaaacgct
     3181 cccatcgaat agccgataag ggttcggccg gcgctttcgt cgacgagggg ccctagcgcg
     3241 cgtatccctc gcaccacggc cgcttcgatc tgctccgagg cggcttccgg ccagatcgag
     3301 ccaccgccat cgcagcgtcg ccgcgcgcgc gggcaaatca agtgctcgtg ttcgctgacg
     3361 agcgcgctga agtaacgaca ggtccgctct ggttcgccgc acatgccgtg cagaaccacg
     3421 gtgacgggcc gaggcccatc ggcgcgcgcc gggtagacga cgacgccagg ctcttcttcg
     3481 gtctcgggca gcgttagcgg cggcgtttgc gccagcgcga tccgcacggg gaggaaaaga
     3541 accacgatgt gaagcacgag gcgcacgacg gcgtgcgcgc ggaacaggcg cgtgagtgac
     3601 tgcgacacag aggtctccct tctcgcgatg tgcgagcgaa cgcgcatcga aagcgctgct
     3661 ctcgcccacc gtgagcagcc gccgttgtcg cgcgcttcac tgattcatcg cgcgagggag
     3721 cggagtgtga cctcgggatc gattcgtttt acgcagtgct cgtcacgagg cgacgtcgtc
     3781 gattcgcgga cggcgccgcg tagtcatcgt gcgtgcccat cgagcgggtc ggtcgcgaac
     3841 ggggcgcgcg cgcgagctac ttggagtcgc cgagtcgacg ccgaggagtt gggagagact
     3901 cgtccttgct gccgcgcaga tcgatgccgc gcggtgcgag gctgctgtcg ctgtctggcg
     3961 ccgggggcgt cgccactttc gacgtgtgcc gacgcggact cacgggcgct gaaaagtcgc
     4021 tcgacgtgcc cttcccgtgc tcgacgggaa cgcgctcggt gctcgagggg agcggccgcg
     4081 gagcgtcact cgaaggaacc ggcgttgggg cgtctgtcga cggcgagggc agggggcgcg
     4141 acgtggctcg acgacgagcc tcggcgactt caggcgcaga gtcgcccgcg ggatcgagcg
     4201 aacgcgcgcg gtcgaggccg gcgatgcact cgtcgactgc accgtcccgg cagcgttcga
     4261 gcgcggcgcg gcgtagtttg cgagcgagtt cgagcggcgc gtcctcgcga gcgacttccg
     4321 gattcgcgtg ctcggcttcg tcgcgatgcc tcgagtagag ataaacgccg agcgatagaa
     4381 ccacaccggc gagcaagagc tcggtcgccc agcgctgtcg caagaagcta cgcagacgcg
     4441 agacgcgttt acgtacgcgc ggggcgggca cgtccgcgtc gcgcgcgatg tgttcgagct
     4501 tttctccatc gccctcacgc agcatccact cgagggtgtg ctgggactcc gaatccggga
     4561 gttcccgctc cgcccatcgc agcaggtcac gcgcggagag cggctctggc gcttgctcgt
     4621 cggcgttggc atcgtcgctc ggaacctcgc gcgctctctt gcgaaagtga tcgaagacct
     4681 tgtgtcgcgc gacgccaaac acgaatcgtc gaaattcttc ttcgtccgcc gggacgtctc
     4741 gcgctgcaag cacctccgcg aacgtggctt gaacgacgtc gagcgcctcg tcgctttgga
     4801 tgcggcggcg cacggcagct tggacctccc tgaacaagtt ggggtcagca agccttcgat
     4861 tcagttcggc cgcttctaag ttcacccgca ctgccatgcg tcgtccccct atcatcgcgc
     4921 gacccgaaag aactgtgacc cgactccgcg aaaagagtcg aggagcggca ttttagtgtc
     4981 actgcgctcc gagcactgcg gcgtgattcg aggagagcct tttacgcgcg gactgccgaa
     5041 gcccttccct gtagacgctg aaaatcagcc cggaagccgc agattgacga ccccgtcgac
     5101 ggcgccgcgg ctcaggctgg aggggagctc gggcaggagc ggggcgagcg ccgcagggat
     5161 gttggaaggg tgcacgacgc cgacctgaat ggttcggctg gtctcgtcga gcaagatgaa
     5221 gaagtagcgg tcgcccttgg ggcgtctcca aacgggacag ccatcgctcg cgccgccggc
     5281 caccacgacc gggcccttgt cataagcttt cttcgcgcac gcatccacga tctcgacgtc
     5341 agtcgggaag gcgttgcgat cacagctcga cttgcacacg gagccgccga cgtgcccggc
     5401 ctggaacgag cgcactcccg tgctcgcgaa ttctcggttc gagtaatcgt tgttgtccat
     5461 gacgacctcg aacacgttga tttcgccgca gccgtcgcca acgct
//
LOCUS       DASTKS010001111         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1291773, whole genome shotgun sequence.
ACCESSION   DASTKS010001111 DASTKS010000000
VERSION     DASTKS010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291773"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFQ35_23485"
     CDS             complement(<1..189)
                     /locus_tag="VFQ35_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933696.1"
                     /translation="MLREHGRLLCRCAYGIVVALLGVAACSSDEQSANAPSVEQQDLS
                     CPTQAIGPSPLKRLTRFEY"
     gene            585..2150
                     /locus_tag="VFQ35_23490"
     CDS             585..2150
                     /locus_tag="VFQ35_23490"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9933697.1"
                     /translation="MSPAAAPPAPLPAGRAAPSKARVKKDSAALPIAPAPTTASPEFN
                     TESYSRIVDNQFVSPRSAPLSTFSVDVDTASYANVRRFIEQGALPPPDAVRIEELVNY
                     FSYDYPDPKGDEPFSVTTELSSAPWNPSHRLLLVGVQGKRIPTRELPPRNLVFLVDVS
                     GSMNEPNKLPLLKYALRELVDSLGAKDRVSLVVYAGASGVVLNPTPASEKQTILSALD
                     RLEAGGGTNGADGINSAYALAERAFDPRGVNRVVLATDGDFNLGVSSEGDLVRLIEEK
                     RTKGVSLSVLGFGMGNYKDSTLEKLADHGNGNYAYIDSRAEARKVLVEQGGSTLVTIA
                     KDVKIQVEWNPAHVESYRLLGYENRALAAQDFNDDKKDAGDIGAGHSVTALYEIVPPG
                     AASTHTNVDPLKYQTSGASNAAASAELGTIKLRYKRPRTDSSILTSVIVKDEARPLAD
                     TSDNFRFASAVADFGMMLRKSEHKGGASYALAHKLAEGARGADRGQYRAGFINLLARA
                     ERLEAPALVKASR"
     gene            2376..>2834
                     /locus_tag="VFQ35_23495"
     CDS             2376..>2834
                     /locus_tag="VFQ35_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933698.1"
                     /translation="MNSARTIARNALLVSFLAFLGLPGAGCGDDGDSGSASGGMAGRQ
                     GGAAGNASGGAVGLKPSGGTNAGGTAGNPSHGGALNGGASSGGQQSFGGAAQSGSGGG
                     GTGGVTEGGAGGEVGGVGPSGGSGGARAEARQCAESCSIDNDCFMDPAMPD"
BASE COUNT          534 a          948 c          914 g          438 t
ORIGIN      
        1 gtattcgaag cgagtgagtc gcttgagtgg cgacgggccg atcgcctgag tggggcagct
       61 caagtcctgc tgctcgaccg acggtgcgtt cgccgactgc tcatcggacg aacacgcggc
      121 gactcccagt aaggcgacca cgatgccgta cgcgcagcgg caaaggagcc ggccgtgttc
      181 tcgcaacatg ccgccactat gtgccccggg tgcaaatccg atcggcattt ctgctcgccg
      241 agcaactgat tctgctcggg gggcgggtgg gagaaaaaga cgacctgagg ctgtaagggc
      301 gcgcgtaccc cggcgttcga ttcgcgtccc gcaacaagga gaacgtcgag atgcgccgat
      361 cgcgttggtt tctgactggt tcagtggttg tgctcgccat ggggtgctcg tacgggcgaa
      421 ccgcggacaa ggcgcccgaa cccaccggcg cctatgcgac gtcagcgact tacgcccagg
      481 ctgaaccgag ggccgacgtg gctaccgaag gcgccaacga gaccgatgag gccgcccctg
      541 aggctccccg agaagaaaaa gctatcgcgg cgggccgtac tccagtgagc ccagcagccg
      601 cgccgcccgc accgcttccc gccggtcgcg ccgctccgag caaagcccgc gtcaaaaaag
      661 atagcgctgc gctcccgatc gctccggcac ccacgacggc ttctcccgag ttcaacaccg
      721 agagctattc acgcatcgtc gacaaccaat tcgtgtcgcc gcgctcggcg ccgctctcca
      781 ctttctccgt cgacgtcgac acggcatcgt acgcgaacgt gcgccggttc atcgagcaag
      841 gcgcgctgcc gccgcccgac gccgtgcgca tcgaagagct cgtcaactac ttcagttacg
      901 actacccgga tcccaaaggc gacgagccgt tctcggtcac cacggagctt tccagcgcgc
      961 cgtggaaccc gtcgcatcgt ttgctcttgg tcggcgttca aggcaagcgc atccccacgc
     1021 gcgaactccc gccgcgcaac ttggtgttct tggtcgacgt ctccgggtcg atgaacgagc
     1081 cgaacaagct cccgctcttg aagtacgcgc tgcgcgagct cgtcgattca ctgggcgcca
     1141 aggatcgcgt gtcgctcgtc gtgtacgcag gcgcgagcgg cgtcgtgctg aatccgaccc
     1201 cggcgagcga gaagcagacc atcctgagcg cgctcgaccg actcgaagcg ggcggtggaa
     1261 ccaacggcgc tgacggcatc aactcggcgt acgcgctcgc cgagcgagcc ttcgacccgc
     1321 gcggcgtaaa ccgagtcgta ctcgcgacgg acggcgactt caacttgggg gtgagcagcg
     1381 aaggcgacct cgtgcgcctc atcgaagaga agcgcaccaa aggcgtgtcg ctcagcgtcc
     1441 tcgggttcgg catgggcaac tacaaggact cgaccttgga gaaactcgcc gatcacggaa
     1501 acgggaacta cgcctacatc gactcgcgcg ccgaggcccg caaggtgctc gtcgagcaag
     1561 gcggttcgac actcgtcacg atcgccaaag acgtgaagat tcaggtcgag tggaaccccg
     1621 cgcacgtcga gtcgtatcgt ctgctcggct acgagaaccg cgctctcgcc gctcaagact
     1681 tcaacgacga caagaaagac gcgggcgaca tcggcgccgg tcacagcgtg acggcgctgt
     1741 acgaaatcgt gccccccggc gcggcgagca cccacaccaa cgtcgatcca ctcaagtatc
     1801 aaaccagcgg cgcgagcaac gcggccgcca gcgccgaact cggaaccatc aaactccgtt
     1861 acaagcggcc acgcaccgac tcgagcattt tgacgtcggt catcgtgaaa gacgaggcac
     1921 gcccgctcgc cgacacgagc gataacttcc gcttcgcgag cgcggtcgcc gacttcggca
     1981 tgatgctgcg caagtcggaa cacaagggcg gcgcgagcta tgccctcgcc cataagctcg
     2041 cggaaggtgc tcgcggtgcc gaccgcggac agtaccgcgc tggcttcatc aacctgctcg
     2101 cgcgcgccga acgcctcgag gcgccagcgc tcgtcaaagc ctcacgctag cgggggtcca
     2161 agcttctgcc ggtcgcagga gccacttctc gtcttcgtct actgaaccgg gctcgaatcg
     2221 acacgcggga aagtcgattc gagcccgatt gctttttgcg actgaccaat cgcgttcacg
     2281 gcgcttgtgc cgcgacggaa cctcttcccc gagtcgggtc gattgcgtaa ggtgagatca
     2341 cccggcgaac ttcgtccgga gtctcagggg cccgaatgaa cagcgctcgc accatcgctc
     2401 gcaatgctct actcgtctct ttcctcgcct tcctcgggct accgggcgcc ggttgtgggg
     2461 acgacggcga ctcgggctca gctagcggcg gcatggcggg gcgacaggga ggcgcggccg
     2521 gcaatgcatc gggaggcgcg gtcggcctaa agccttcggg gggcaccaac gccggtggca
     2581 cggctggaaa cccgagtcac ggtggagcgc tgaacggcgg cgcttcctcg ggcgggcagc
     2641 agtcgttcgg cggagccgcc cagagcggaa gtggcggagg cggtaccggc ggcgtcaccg
     2701 aaggtggcgc aggcggagaa gtcggcggcg tcggcccgag tggaggcagc ggcggtgcgc
     2761 gcgccgaagc acgtcagtgc gcagagtcgt gctccatcga caacgactgc ttcatggatc
     2821 cggcgatgcc cgac
//
LOCUS       DASTKS010001112         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1292716, whole genome shotgun sequence.
ACCESSION   DASTKS010001112 DASTKS010000000
VERSION     DASTKS010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292716"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VFQ35_23500"
     CDS             <1..1641
                     /locus_tag="VFQ35_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933699.1"
                     /translation="ALLRLVPPAAGSKRAFIAFERLEARLSTQRYFSLSALDEAGRLS
                     GNRAELGYAKDDGSVPEFAATPDGLAALTVAPVCRLHASCEGSNTSPTYVRFDGSMKV
                     VASEPLRLEPLAGESAELGFGLGCWGAKCVGISALTKVPAPIFAVQLEPRSSVWAAPV
                     RDVERAQPPRVKEFESVALAESVARLSVASAGGQDFVAYITDFDDNTPWKLLDRPAAD
                     GRREPLRAQISLLRTNPSERRREPTQVSLRAHAVGGVALAPGDASRGELAVAWAGLDA
                     GVPQLFLTLFDKNGTKLSQRMITHKKGDLSDIALTWVGDGWVLAWVDERSGDAEVYAT
                     KVDPKLNRTAPEQRITSSSGVASDLAIAFDGKAVELAWSDARGADAGAHGDIYTARLR
                     PRDAAREGDERRVAATRAHSFSPALQPARDGFALAWIERGDDSDTGRVAFVDIGKDGS
                     AGPLSTVPLPSGTEPRTVALDCPSATCRFLVTADGNDQGELFGGVWQPGKLQPLKALL
                     PLADGSAASAAPVLRGDIAWLADKATDRTRVRKLRMEW"
     gene            2042..>2880
                     /locus_tag="VFQ35_23505"
     CDS             2042..>2880
                     /locus_tag="VFQ35_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933700.1"
                     /translation="MVVDTLSAELERLYELDELKSLSSGLLGLDPNEVGGTAAKASFA
                     RALTQRCLEIGALEALLDAVQASHKTLPVDLLKKLRNGALDAAVRPHEGDEVGEFLVL
                     RELGESASGNVYRARHGSEDLRVRRLSANTQERRRDVQRYLAAARIVGGIAHPSLPVA
                     VRAGALEPSGRFFGVAHEFQEGEPLSKLIESRGGRHLNELLPVLWAVLEALGALHSRG
                     LAHGALHAGNVLVSDFGSQQPRVLLLDAGAHHLRPGLFGLGAAGVPSWLTTASPELLR
                     GEPV"
BASE COUNT          423 a          959 c         1008 g          490 t
ORIGIN      
        1 gcgctgttgc gtctcgttcc gcccgccgcg ggcagcaagc gcgccttcat cgcgttcgag
       61 cggctcgagg cgcggctctc gacgcaacgc tacttctccc tttcggccct cgacgaggct
      121 gggcgactca gcggaaatcg cgccgagctc ggctatgcaa aagacgacgg cagcgtgccc
      181 gaattcgcgg ccacccccga cggcctcgcc gcgctcacgg tcgcgcccgt ctgtcgcctg
      241 cacgcctcgt gcgaggggag caatacgagc cccacgtacg tgcgcttcga cggctcgatg
      301 aaggtcgtcg cgagtgagcc actccgtttg gagccgctcg ccggtgaaag cgccgagctt
      361 ggatttggtc ttggttgttg gggagcgaag tgcgtcggta tttcagcgct gacgaaggta
      421 ccggcgccga tcttcgcggt gcagctcgag ccgcgctcgt ctgtctgggc ggcgccggtg
      481 cgtgacgtcg agcgagccca gccgccgcgg gtgaaggagt tcgagtcggt agctctcgcg
      541 gagtcggtcg cgcggctctc cgtcgccagc gccggcgggc aggacttcgt cgcgtacatc
      601 accgacttcg acgacaacac gccgtggaag ctcctcgacc gccccgcagc ggacgggcgc
      661 cgggaaccgt tgcgcgccca gatatcgctg ctccgaacta acccttcgga gcgtcgtcgc
      721 gagccgaccc aagtttcgct gcgcgctcac gcggtgggcg gcgtcgcgct cgcgcccggc
      781 gacgcatctc gtggtgagct cgccgtggcg tgggccgggc tcgacgccgg cgtcccgcag
      841 ctcttcttga cgctcttcga caagaacggg acgaagcttt cgcagcgcat gatcacccat
      901 aagaagggcg acctttcgga catcgcgctc acgtgggtag gcgatggctg ggttctcgcc
      961 tgggtcgacg agcgcagcgg cgacgccgag gtgtacgcga ccaaggtcga tcccaagttg
     1021 aatcgcaccg cgccagagca gcgcatcacg agctcctccg gcgtcgcctc ggatctcgcg
     1081 attgcgttcg acggcaaggc ggtcgagctc gcctggtcag acgcgcgcgg tgctgacgcc
     1141 ggcgcgcacg gcgacatcta tacggcgcgg ctccgcccgc gcgacgctgc tcgcgaaggc
     1201 gacgagcgcc gcgtcgccgc gactcgggcc cactcgttct cgccggcgct gcagcccgcg
     1261 cgcgatggct ttgcgctcgc ctggatcgaa cgtggcgacg acagcgacac cggacgcgtc
     1321 gccttcgtcg atatcggtaa ggacggaagc gcagggccgc tctcgacggt gcccctgccg
     1381 agcggcaccg agccgcgcac ggtggccctc gactgcccga gcgcgacctg ccggttcctc
     1441 gtcaccgccg acggcaacga ccagggcgaa ttgtttggcg gcgtctggca gccaggaaag
     1501 ctccagccct tgaaagcgct tctgccgctc gccgacggct ccgcggcgag tgccgctccc
     1561 gtccttcgcg gcgacattgc ttggcttgcc gacaaagcca cggatcgaac gcgtgttcgt
     1621 aagctccgta tggaatggtg aggcgacccg cttcggcgag gtctcacccg ggtgaaatat
     1681 tccagctcag taacgcccat ccctgcgcca gagaggtcgc aggtcgcgag cgggtgaccc
     1741 cttcgtctgg cgcggggcgc tgaaagctgg ccgtggaagg gttggtttcc agctgcgctg
     1801 cgaaagagca tcgttgcaat gcttggttgg gttggttttt ggggcctcaa agtccctgga
     1861 aactgcgctt tgatgccggg ttctgtgact gatgactcgt gatgagtgca ccacgactgg
     1921 aacccgcgag agcgcgctgg taagtctggg ctcgtgattg aaccgaggcc cggagactgc
     1981 gaccgctcac cggtgggtgg gcgggggtgc gcttgacgga gcccggccga ggagcagcag
     2041 gatggtcgtc gacacgctga gcgcagagct ggagcggctg tacgagctcg atgagctcaa
     2101 gtcccttagc tcaggactct tgggtctgga tccgaacgag gttggaggaa ccgccgccaa
     2161 ggcttcgttc gcgcgcgcgc tcacccagcg ctgcctcgag atcggtgcgc tcgaggcgct
     2221 tttggacgcg gtgcaggctt cgcacaagac actccccgtc gatctcctga agaagctgcg
     2281 taacggcgcg ctcgacgccg ccgttcgtcc gcacgaaggc gacgaggtcg gtgagttctt
     2341 ggtgctgcgc gagctcggcg agagtgcgag cggcaacgtg taccgcgcgc ggcacggcag
     2401 cgaagatctt cgcgtgcgcc gtctgtcggc gaacactcaa gagcgacgtc gcgacgttca
     2461 acgttacctc gccgcggcgc gcatcgtcgg cggcatcgcg cacccgtcgt tgccggtggc
     2521 ggtgcgagct ggcgcgctcg agccgagcgg gcgctttttc ggcgtcgccc acgaatttca
     2581 ggagggtgag ccgctcagca agctcatcga atctcgcggt gggcgacacc tgaacgagct
     2641 cttgccggtc ctgtgggccg tgctcgaggc gctcggcgcg ctgcactcac gagggctcgc
     2701 ccacggcgcg ctccacgccg gcaacgtgct ggtgtccgac ttcggctcgc agcaaccgcg
     2761 cgtgctgttg ctggacgccg gcgcgcacca tttgcggccg ggcctgttcg ggctgggcgc
     2821 ggcgggcgtg ccgagctggc tcacgaccgc ttcgcccgag ctcttgcgcg gcgagccggt
//
LOCUS       DASTKS010001113         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_175794, whole genome shotgun sequence.
ACCESSION   DASTKS010001113 DASTKS010000000
VERSION     DASTKS010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175794"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1902
                     /locus_tag="VFQ35_23510"
     CDS             247..1902
                     /locus_tag="VFQ35_23510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9933701.1"
                     /translation="MLSLPRLRAEDAVVPLSASESASPDAVRGACVSLIGIGLVACAL
                     VYFGPASFGFERFSVPKEFVLALSATSALVLFLSDGRAFKSSRALLALSGLLVVGCLA
                     TWRGVTSEGYAVRSLSALISAVTVFLLAFALATEGHGPRLLKWAALATVLAALSALGE
                     ALHLLPVISMSGRGPGGTAGNRNFLSHFLVIGAVPLALTFVEARGKVARAAWALGFAC
                     VVAIIAVSRSRGSWLTSSIVLVGVAAVLAAKWRSLSRDARRTSAVLAVAITVAVPVGV
                     FLIARGLNWTFADFRSSLAHLVDFRTGTGHGRLLQYQATLTMLRDHPVAGVGPGQWSI
                     AYPRYAGPGDPYYPANSPVPFDRLPSSDWVGFASQHGLLLLLVVVALGATLAGEALRR
                     GLLRDRHATALAAALVAQLVLGVFDSVLFRADTVYIAALSIGTLAGSCADEVFVLRAP
                     RAPALGLALVLSVTLVALCGRRVAAWSIRSDGRLPNLERSLSLDRSNFVLASQIAQIH
                     SALGHCDDAKAFARAALDACPTASLPKKILLSCGSLQASLSAK"
     gene            complement(1993..>2517)
                     /locus_tag="VFQ35_23515"
     CDS             complement(1993..>2517)
                     /locus_tag="VFQ35_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933702.1"
                     /translation="GVDRRFRDWWLGLLERYRGERPAKLDGATTLVRFSRLNAPDWQA
                     IRDGRWLPGPVRGADGTATVRELDGATAVWGFRLLKDDTGRYVVEVVHSSAVGAPSRL
                     SPAAPAAPSAELSQALSAEQRAEQGPNLGPPDPEFPRIDLMVDGSVSGALFARRRDGW
                     HIWKLSYPDATREL"
BASE COUNT          340 a          830 c          857 g          490 t
ORIGIN      
        1 cgtggactgc tgcgacagcg actcgagcgt gagaccggga cagggcacgc cgtcctccaa
       61 gtcgagcagc tgcgggacct gggacttcaa ttgcgacggc aaggcggtcc agtccgatct
      121 cacgcaaggt agcgcgggag actgctcgca gggcttgggg tgggcaggga cgccgccggc
      181 gtgtggacag tcagggtcgt acatcgtcgg ctgtgatccc ccttcgagct gcggagagac
      241 cggctcgtgc tctccctccc ccgtcttcgc gccgaggacg cagtcgtgcc tttgagcgcg
      301 agcgaatcgg cgtcgcccga cgcggttcgc ggcgcttgcg tgtcgctgat cggcatcggg
      361 ctcgtcgcgt gcgcgctcgt gtacttcggt ccagcgtcgt tcggcttcga gcgattctcg
      421 gttccgaagg agttcgtttt ggcgctcagc gcgacgagcg cgctcgtgtt gttcttgagc
      481 gacggacggg cattcaagag cagccgcgcg ctgctggcgc tttcgggcct cctcgtcgtg
      541 gggtgcctcg cgacttggcg cggcgtcacc agtgagggct acgcggttcg gtccctgagc
      601 gccttgattt cggcagttac cgtgtttctg ctggcgttcg cgttggctac tgaagggcac
      661 ggccctcggc tgctgaaatg ggctgctctg gccacggtgc tcgcggcgct cagcgccctc
      721 ggcgaggcgc ttcaccttct gccagtgatc tcgatgtctg ggcgagggcc cggcggaacg
      781 gcgggcaacc gcaacttcct gtcgcacttt ctggtcattg gtgcggttcc gctcgcgctc
      841 acgttcgtgg aagcgcgcgg taaagtagct cgcgctgcgt gggcgctcgg gttcgcgtgc
      901 gtcgtcgcca tcatcgcggt ttcgcgaagc agaggttcgt ggctgacgag ttccatcgtc
      961 ctcgttggcg tcgcggccgt gctcgcggca aagtggcggt ctctatcgcg cgacgcgagg
     1021 cgaacttcgg cggtgctcgc ggttgccatc acggtagcag ttccggtcgg cgtgtttctg
     1081 atcgcgcgcg ggttgaattg gaccttcgcc gatttccgtt cgagcttggc gcacctcgtc
     1141 gacttcagga caggtaccgg gcacggtcgc ctgcttcaat accaggccac cctcacgatg
     1201 ctccgagatc atcctgtagc gggagtgggg ccggggcagt ggtccattgc ttatccgcgc
     1261 tacgcggggc caggggatcc gtactatcca gccaacagcc cggtgccgtt cgatcgcctt
     1321 ccgagctctg actgggtggg cttcgcgtcc cagcatggcc tcctgctcct gctcgtggtc
     1381 gtggcgctgg gtgcaactct cgcaggtgag gcgctgcgtc gtgggttgct tcgcgatcgc
     1441 catgcgaccg cgcttgcggc cgccctcgtg gcccagttgg tgctcggtgt cttcgattcg
     1501 gtgctgtttc gtgccgacac cgtgtacatc gcggccttga gcatcggtac gctcgctggc
     1561 tcgtgcgccg acgaagtctt cgtgcttcgc gcaccgcgcg caccggcgct cggcttggcg
     1621 ctggtcctct cggtgacgct ggtcgccctt tgcggtcgac gggtcgccgc atggtcgatt
     1681 cgcagcgacg gaaggctgcc gaacctcgag cgttcgttga gcctcgatcg cagcaatttc
     1741 gtgctcgcct cgcagatagc gcagattcac tctgcgctcg ggcactgcga cgacgcgaag
     1801 gcgttcgctc gcgccgccct cgatgcgtgt ccgaccgcga gcctcccgaa gaagatcctg
     1861 ctctcctgcg gctccctgca ggcttcgctg agcgcgaaat agcgggcccc aacaagagga
     1921 tcggcccgcg ccgtatgctg cccctgcgct ttttggcgac gtgccgctcg cgccgtaccc
     1981 attcgcaggc cctcagagct cgcgcgtcgc atcgggatac gatagcttcc aaatgtgcca
     2041 gccgtcgcgg cgccgcgcga acagagcgcc gctcacggag ccgtcgacca tcaaatcgat
     2101 gcgcggaaat tccggatcgg gaggtccgag attgggacct tgctccgctc gctgttcggc
     2161 agacaacgct tgcgagagct cagccgacgg cgcggcgggc gccgcgggcg aaagcctcga
     2221 gggcgcgccg accgcgctgg agtggacgac ctccaccaca tagcggccgg tgtcgtcctt
     2281 tagcaggcga aagccccaaa cggctgtcgc gccgtcgagc tctcgaacgg tcgcggtacc
     2341 gtcagcgcca cggacggggc caggcagcca acgcccgtcg cgaatcgctt gccagtccgg
     2401 cgcgttcaac cgggagaaac gcaccagggt cgtcgcgccg tcgagcttcg cggggcgctc
     2461 gccccgatat cgttcgagca agccgagcca ccaatcgcga aagcgccggt cgactcc
//
LOCUS       DASTKS010001114         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_621580, whole genome shotgun sequence.
ACCESSION   DASTKS010001114 DASTKS010000000
VERSION     DASTKS010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621580"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFQ35_23520"
     CDS             complement(<1..402)
                     /locus_tag="VFQ35_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933703.1"
                     /translation="MSPQKLLTSLRIYVGVLFALSLAIYSYLHIARKEGAPREYIVSV
                     WRGGARQARVVVAADPEKQLGDAASEPGASLVIEDVIDEGPVLSSSPFLFGMSFVPGR
                     DAIRAALGGRVAYATPDDLLKLGAYELFVPFG"
     gene            complement(443..925)
                     /locus_tag="VFQ35_23525"
     CDS             complement(443..925)
                     /locus_tag="VFQ35_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933704.1"
                     /translation="MSELMVPSRERRWQVGLAIANLLPAVVLSGGCYLLPLRWWAMDF
                     SVGAVVALLVVTSVLSFAKPGLALRALRVAGIALLALGAGLLAAFVLCLAFVSGIHGA
                     FGNLGAVLMTLVILLIAPYALVYPVCELLVVHRLQRAHGPVPTAAESVPAVAAEAGEA
                     "
     gene            complement(922..>2943)
                     /gene="purL"
                     /locus_tag="VFQ35_23530"
                     /gene_synonym="purI"
     CDS             complement(922..>2943)
                     /gene="purL"
                     /locus_tag="VFQ35_23530"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET9933705.1"
                     /translation="SNISPADAAERVLRMPGVASKSFLITIGDRTVTGLVARDQMVGP
                     HQVPVADAGVSLVDYEGYAGEALAIGERAPVALLSPEASARLAVAEAITNLCSTSVSS
                     FADVRLSANWMAAAGEPGEDAALYDAVRAVGMELCPALGLSIPVGKDSMSMRTLWEGG
                     KKRVVAPLSLVVTAFAKIDDVRRTVTPELEAKSDDGALVLIDLGAGKNRLGGSALAQA
                     FEAMGGEPPDLDRPELLKAFFEATRELMRENLVHSYHDRSDGGLFVTLCEMAFASGFG
                     LTVDLSEVGSDPLAAMFSEELGAVLEVPAERLDDVFAILARHGLKRGPGCFSQLLGTP
                     GPSEHLVFKYAGTDYFVASRPELKRAWCETSYRMQALRDNSDCADQEFQFSCDPDDLG
                     MIPHVSFDIEVGLEPSLLSVKSHERPRIAVLREQGVNGQVEMAAAFTRAGFAAVDVHM
                     SDILSGRVSLESFRGLAACGGFSYGDVLGAGQGWAKSILFSARAREAFSAFFRDPRRF
                     ALGVCNGCQMMSALKDLIPGAEAWPRFVRNKSEQFEARLSMVEVPQSPSILFAGMAGS
                     RVPVAVSHGEGYAQFAKPEDLARCEAERLVALRFVDSRGRHTEHYPQNPNGSPAGITS
                     LTTPDGRVTVLMPHPERVFRAVQLSYHPDDWRERSPWMRLFDNARRFVG"
BASE COUNT          569 a         1002 c          974 g          398 t
ORIGIN      
        1 gccgaaggga acaaaaagct cgtaagcgcc gagcttcagc aggtcatccg gggtcgcgta
       61 cgcaacgcgc ccacccaacg ccgcgcgaat ggcgtcgcgc cctggcacga acgacattcc
      121 gaacaagaac ggcgacgagg agagcaccgg tccctcgtcg atgacgtcct cgatgacgag
      181 cgaagcgccg ggctcggagg cggcgtcgcc gagctgcttt tcagggtcag cggccacgac
      241 gacgcgcgct tgacgcgcac cgccgcgcca cacgctgacg atgtattcgc gcggcgcgcc
      301 ctctttgcgc gcgatgtgca ggtacgaata aatggcgagg ctcagggcga acaacacccc
      361 gacgtagatt cgcaggctgg taaggagctt ctgcgggctc acgaacgcga gccttccgcg
      421 cgctcgaacg gagccgagcg tctcatgctt ccccggcttc ggccgccacc gccggcacgc
      481 tctcggcagc cgtgggcacg ggcccgtgag cgcgctgcaa gcgatgcacg accaggagct
      541 cgcacaccgg gtaaaccagc gcgtagggcg cgatcagcaa aataacgagc gtcatcagca
      601 ccgcgcccaa gttgccgaag gcgccgtgaa tgcccgaaac gaaggcgagg caaagcacga
      661 acgcggcgag cagccccgcc ccgagcgcga gcagagcgat gccagcaacg cggagcgcgc
      721 gcaaagcgag gccaggcttg gcgaacgaga gcacgctcgt cacgacgagg agcgcgacca
      781 cggctccaac cgagaagtcc atcgcccacc agcggagcgg caagaggtag cagccgccac
      841 ttagaacgac cgcggggagc aagttggcga tcgcaagccc cacttgccag cgccgctcgc
      901 ggctcggaac catgagctcg ctcatccgac gaagcgcctt gcgttatcga acagccgcat
      961 ccacggcgaa cgttcacgcc aatcgtcggg gtggtacgaa agctggaccg cgcgaaagac
     1021 gcgctccggg tgcggcatca acacggtcac gcggccgtcg ggggtcgtga gcgacgtgat
     1081 gccagcgggc gagccgttcg ggttttgggg gtagtgctcg gtgtgacggc cgcgtgagtc
     1141 gacgaaacgg agcgccacga gccgctccgc ttcgcaacga gcgagatcct cgggcttcgc
     1201 gaactgcgcg tagccttcgc catgcgagac cgcgaccggg acgcgcgagc cggccatccc
     1261 cgcgaagagg atggacggag actgtggcac ctccaccatc gaaaggcgcg cctcgaactg
     1321 ctccgacttg ttgcgcacga agcgcggcca ggcctcggcg ccgggaatca agtccttcag
     1381 cgccgacatc atctggcagc cgttgcaaac gccgagcgcg aagcgccgcg ggtcgcggaa
     1441 aaacgcgctg aacgcctcgc gcgcgcgcgc gctgaagagt atcgatttgg cccagccctg
     1501 ccccgcaccg agcacgtccc cgtaagagaa cccgccgcac gcggcgagcc cgcggaacga
     1561 ctcgagcgac acccgacccg acaagatgtc gctcatgtgc acgtcaaccg cggcaaagcc
     1621 ggcgcgcgtg aacgccgcgg ccatctcgac ctggccgttg acgccctgct cgcgaagcac
     1681 cgcgatgcga gggcgctcat ggctcttcac cgagagcaac gacggctcga gcccgacctc
     1741 gatgtcgaag ctcacatgcg gaatcatccc gagatcgtcc ggatcgcaag agaactggaa
     1801 ctcttggtcg gcgcagtcgg agttgtcgcg cagcgcttgc atgcggtagc tggtctcgca
     1861 ccaggcgcgc ttcagttccg gacgcgaggc gacgaagtaa tcggtccccg cgtacttgaa
     1921 gaccaagtgc tcgctcgggc ctggggtgcc gagtagctga ctgaagcagc cggggccgcg
     1981 tttgaggccg tggcgggcga ggatcgcaaa gacgtcgtcg agacgctcgg ccggcacttc
     2041 gagcacggcg ccgagctctt ccgagaacat cgccgcgagc ggatcactgc cgacctcgct
     2101 gaggtcgacg gtgagcccga accccgacgc gaacgccatc tcgcagagcg tcacgaagag
     2161 accgccgtcg gagcggtcgt gataggagtg caccaggttt tcgcgcatga gctcgcgcgt
     2221 cgcctcgaag aacgccttca agagctcggg tcgatccaga tccgggggtt cgcctcccat
     2281 cgcttcgaac gcttgtgcga gcgcagagcc gcccaagcgg ttctttcctg cgccgagatc
     2341 gatgagcacg agcgcgccgt cgtccgactt cgcttcgagc tccggcgtga ccgtgcggcg
     2401 cacgtcgtcg atcttggcga acgcggtgac caccagcgac agcggcgcga ccacgcgctt
     2461 tttgccgccc tcccacagcg tgcgcatgct catcgagtct ttgccgactg ggatcgacaa
     2521 cccgagcgcc gggcagagct ccattccaac cgcgcgcacg gcgtcgtaga gcgccgcgtc
     2581 ttcgccgggc tctcccgcgg ccgccatcca gttcgccgaa agacgcacgt cggcgaaact
     2641 ggaaaccgac gtcgagcaaa ggttggtgat ggcctcggca accgccagac gcgccgacgc
     2701 ttccgggctc agcaaagcga ccggagcgcg ttcaccgatg gcgagcgctt cgccggcgta
     2761 gccttcgtag tcgacgagag agacacccgc gtcggccacc ggaacttgat gcggccccac
     2821 catctgatcc cgcgcgacga gccccgtcac cgtgcgatcg ccgatggtga tgaggaacga
     2881 cttggaagct acgccgggca tgcggagcac gcgctcggct gcatcggcag gcgaaatgtt
     2941 cga
//
LOCUS       DASTKS010001115         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1293586, whole genome shotgun sequence.
ACCESSION   DASTKS010001115 DASTKS010000000
VERSION     DASTKS010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293586"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFQ35_23535"
     CDS             complement(<1..139)
                     /locus_tag="VFQ35_23535"
                     /inference="COORDINATES: protein motif:HMM:NF012547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL30 family ribosomal protein"
                     /protein_id="HET9933706.1"
                     /translation="MSTLARLEVKQVVSEIGRPHPQRRVLRGLGLRGPGSVVVVDNTP
                     SF"
     gene            complement(229..714)
                     /gene="rpsE"
                     /locus_tag="VFQ35_23540"
                     /pseudo
     CDS             complement(229..714)
                     /gene="rpsE"
                     /locus_tag="VFQ35_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(727..1086)
                     /gene="rplR"
                     /locus_tag="VFQ35_23545"
     CDS             complement(727..1086)
                     /gene="rplR"
                     /locus_tag="VFQ35_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9933707.1"
                     /translation="MGAKIEGRERRKLRIRGRITGTEERPRLSVFRSGRHIYAQVVLD
                     TEGRTLALASTLSRDLKGTLGEDNKSDAARKVGALIAKMCLAKNIEKVVFDRNGYLYH
                     GRVKALAEAAREAGLQF"
     gene            complement(1089..1676)
                     /gene="rplF"
                     /locus_tag="VFQ35_23550"
     CDS             complement(1089..1676)
                     /gene="rplF"
                     /locus_tag="VFQ35_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9933708.1"
                     /translation="MSTPQAEPKRLSRVGKRPVALPKGVNANVTGSRIDIQGPKGKLS
                     LDLPPQVTCKKDGEKLLVESSASGRDGARLQGLARALIASRVKGAAEGYERQLELVGT
                     GYRCEVKGTQVTLTVGLSHPMSIQLPPGVTGNVPADSKGTMLVLSSADKAALGQIAAT
                     IRDVRPPEPYGGKGIRYRGENIRRKAGKAAKGKGK"
     gene            complement(1676..2074)
                     /gene="rpsH"
                     /locus_tag="VFQ35_23555"
     CDS             complement(1676..2074)
                     /gene="rpsH"
                     /locus_tag="VFQ35_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9933709.1"
                     /translation="MVNDPVADMLARIRNASLARHELTRLPASKLKRSIAELLKAEGY
                     VADVRQEEWGEDKRQTLTIVLKYKERHSAFEGMRRVSRPGRRVYVRHDQIPRVLSGLG
                     VSILSTSHGLMTDREARKRKLGGELLCEVW"
     gene            complement(2080..2265)
                     /locus_tag="VFQ35_23560"
     CDS             complement(2080..2265)
                     /locus_tag="VFQ35_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9933710.1"
                     /translation="MARASQWAKLNRPAKFSTRIVNRCKTCGRSRAVYRDFGLCRLCF
                     RGLALKGELPGVTKASW"
     gene            complement(2272..>2460)
                     /locus_tag="VFQ35_23565"
     CDS             complement(2272..>2460)
                     /locus_tag="VFQ35_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9933711.1"
                     /translation="SPRGFDGAGNYTLGLKEQIVFPEIDFDKVDKVKGMNITFVTSAE
                     TDEEAKELLGSLGMPFRS"
BASE COUNT          403 a          816 c          798 g          443 t
ORIGIN      
        1 ggaaggaggg cgtgttgtcc acgaccacga ccgaaccggg gccgcgcaga ccgagcccac
       61 gcagcactcg gcgctgcggg tgcgggcggc cgatctcgct aacgacctgc ttgacctcga
      121 gacgcgctag cgtgctcatc ctgcgcctcc accagactgc gtgctcgcga cgatcgaagc
      181 cgcggtgctc gcttgagccg aagcggccgc gaccgggtag tccgccgcga cgtgatcgag
      241 ctctttgccg cgggcgtcag cgacgcgctc ggccgagcgg agcttgcgca gcgcctccac
      301 ggtggcgtgc acgacgttgt acttgttcga cgatcccaag cacttgctca agatgtcgtg
      361 aatgccggcc gactcgacga cggcgcggac ggcgccgcca gcgataacgc cggtaccagg
      421 agcggcgggg cggaggagca cccggccgga gccgaactca cccgtcacgt cgtgcggaac
      481 cgtcgcgccg accatcggca ccttgaacat gttcttacgc gcctggtcgt tgcccttgcg
      541 gatcgcctcg gggacctcgt tggccttgcc gagaccgacg ccaacgtgac cagcctcgtc
      601 gccaacgacg acgagcgcgg agaagctgaa acgccggccg ccctttacga ccttcgcaac
      661 gcggttgatg tggatgacgc gctctttgag cttctcgtcg tcaatgtgtt ccattgcgat
      721 tggtccttag aactgaaggc ctgcttcgcg ggccgcttcc gcgagcgcct tgacgcgccc
      781 gtgatagaga tagccgttgc ggtcgaacac gaccttttcg atgttcttcg ccaggcacat
      841 cttggcgatg agtgcgccga ccttgcgagc cgcgtcgctc ttgttgtcct cgccgagcgt
      901 gcccttgagg tcgcgcgaca aggtggaggc caacgccagg gtgcggccct cggtgtcgag
      961 gacgacctgg gcgtagatgt gacgaccgga gcggaacacc gagaggcgcg gacgctcctc
     1021 ggtgccggtg atccggccac gaatacggag cttacgacgc tcccgacctt caatcttcgc
     1081 gcccatgact acttgccctt gcccttggca gctttaccgg ccttgcgacg aatgttctct
     1141 ccgcggtagc ggatgccttt gccgccgtac ggctccggcg gacgaacgtc gcgaatcgtc
     1201 gcggcgatct gaccgagcgc ggctttgtcg gccgagctca ggaccagcat cgtccccttg
     1261 gagtcggccg gcacgttgcc ggtgacgccc gggggcagct ggatcgacat cgggtgcgac
     1321 aagcccacgg tgagagtgac ctgcgtgccc ttcacttcgc agcgatagcc ggtgccgacg
     1381 agctcgagct ggcgctcgta gccttcggcg gcgcccttca cgcggctcgc gatgagcgca
     1441 cgggcgaggc cctgcaggcg cgcaccgtcg cggcccgagg cggacgactc gacgagcagc
     1501 ttctctccgt ccttcttgca ggtgacctgc ggcggaagat cgagcgagag cttgcccttc
     1561 gggccctgaa tatcgatacg cgagccggtg acgttcgcgt tgacgccctt cggcagcgcc
     1621 accgggcgct tgccgacgcg cgacaggcgc ttcggctctg cttgcggcgt agacatcacc
     1681 acacctcaca caggagttcg ccgccgagct tgcgcttgcg cgcctctcgg tcggtcatga
     1741 ggccgtgcga cgtgctcagg atcgaaaccc cgaggcccga cagcacgcgc ggaatctgat
     1801 cgtggcgcac gtacacgcga cgaccgggac gcgacacgcg acgcattcct tcgaacgcgc
     1861 tgtgacgctc cttgtacttg agcacgatgg tcagagtctg gcgcttgtcc tcgccccact
     1921 cttcttggcg cacgtcggcg acgtagccct cggccttgag caactccgcg atgctacgct
     1981 tgagcttgct tgcaggcagg cgggtgagtt cgtggcgcgc gagcgacgca ttgcggatgc
     2041 gcgcgagcat gtctgcgacg ggatcgttga cgatagccat caccagctcg ctttcgtgac
     2101 gcccggcagc tcacccttga gcgccaaacc gcggaagcaa agacggcaca agccgaagtc
     2161 acggtacaca gcgcgcgaac ggccgcacgt tttgcaacgg ttgacgattc gggtcgagaa
     2221 cttggcggga cgattcaact tcgcccattg agaagcgcgt gccatctaaa ctcagctcct
     2281 gaacggcatg cccaacgacc cgaggagctc tttggcttcc tcgtcggtct cggcgctggt
     2341 tacgaacgta atgttcatgc ccttgacctt gtcgaccttg tcgaagtcga tctcggggaa
     2401 aacgatctgc tctttgaggc ccagcgtgta gttgccggcg ccatcgaagc cgcgggggct
//
LOCUS       DASTKS010001116         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_622592, whole genome shotgun sequence.
ACCESSION   DASTKS010001116 DASTKS010000000
VERSION     DASTKS010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622592"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..376)
                     /locus_tag="VFQ35_23570"
     CDS             complement(218..376)
                     /locus_tag="VFQ35_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933712.1"
                     /translation="MQETAAQAARVGVRLARLYGKPMTAGERMALAHLLDRCVELVQP
                     ALVRRAVG"
     gene            complement(715..1011)
                     /locus_tag="VFQ35_23575"
     CDS             complement(715..1011)
                     /locus_tag="VFQ35_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET9933713.1"
                     /translation="MIKLPKQRMRVDALSYIKTHVERPASIEWLRKAFRETPHLVPPI
                     GLVHQGKHYKLHDGNHRLTVARELGLETIEVVIEFPALTELARNDVRFAHPGAR"
     gene            complement(1008..1385)
                     /locus_tag="VFQ35_23580"
     CDS             complement(1008..1385)
                     /locus_tag="VFQ35_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933714.1"
                     /translation="MPLLRIDGNDVLGKYVRAMHAFEAKACNFNCDDIRRCEAELTRD
                     PLTVIVLERLTQSAALRTVGAKCDEHGHIIPIVGDGPDDHHRVVAALRLGFKEINVRW
                     TDRRAREAMAQAAERYGFPGGRS"
     gene            complement(1393..1689)
                     /locus_tag="VFQ35_23585"
     CDS             complement(1393..1689)
                     /locus_tag="VFQ35_23585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933715.1"
                     /translation="MNYPLDLSHEETLMLSDLDEYEAIAEAGLSTPDMDTHFGEVRAE
                     LERVQRNRRVLADMKAGQKELARQRRLARGVPAALGEALARPAPARRENIGRYR"
     gene            complement(1686..>2858)
                     /locus_tag="VFQ35_23590"
     CDS             complement(1686..>2858)
                     /locus_tag="VFQ35_23590"
                     /inference="COORDINATES: protein motif:HMM:NF021627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HET9933716.1"
                     /translation="HAHPQFHAGPDGVGDKPTIPFDYEHASELSATEGSIPTSGAPAP
                     AWVLDLEVRDGSNGPELWALAALGDQIRQQIAANEYRFTSIAFTLDGTDGVSGASVGP
                     MLTSIAFTNHPFLRGLKPLQIAASARGLAARRVLLGPPVANDATEPADEEGGYDRPAV
                     NLTGTNVDVAAPDAIDQAIEHFKLDPRFAALSRAEQIAYAAACLELTKKNKAERTWLE
                     PDQEGNPTMSTNENPCGLTDEEFAICRRSKTAFSAAHQILALRDRAYAARPDLPQHCR
                     KAHALEQELEAAETEYQAAFRRCEPVVSIGLANAQSVAPPRVAVTPMPKNSITLEQLR
                     ALYPGANDVLKAVEYMRQVAPPFAKFPWDQQVKRAADFVRSLLPTSSTEPAVGGVA"
BASE COUNT          449 a          959 c          910 g          540 t
ORIGIN      
        1 gccagtcctc ggccacgagc aacccatcgc cgatccgcct cgcgatggaa agcctctcgc
       61 agtaatggag cgaatccgat cacttgcggc acacccaccc cgcgatccgc tctcccgcgg
      121 cgaggggcct gaacggttac gtctacaaca ccgcccccga cgcccggcgc ttgacgctcg
      181 cttcgcgttg atggccggtc atcaacgaaa ccccctttca gccaaccgca cgacgcacga
      241 gcgccggttg aacgagctcc acgcatcggt cgagtaagtg cgcgagcgcc attcgttcgc
      301 ccgcggtcat cggtttgccg tagagcctcg caagccgcac accaacgcga gccgcttgcg
      361 ctgcagtttc ctgcaccgag tcgaccttca gacgtttccc gcttcgcttc cgtggtctgc
      421 tgcacacctc gatgcagtgc agacggcgcg cgcacgccgt gagcagcgcg cggactgaag
      481 tgctactcgc cggacaaccg ggctcggggc acacgaacgt cgtcgcgggt gcacccggcc
      541 gcggaattgc gaagccgtgc gcgcactctc gtgttcggtg ttccgtcgac gctcgctcga
      601 tggtggcgaa ctcgtggtca gtgccgtcgc tttcgcttgg tccggcgggc attccctcga
      661 cagcacgcgc cggacacgcg caccggaccg tgacatcgac cgagggctct gacatcagcg
      721 tgcccccgga tgcgcgaacc gcacgtcatt gcgtgcgagc tcggtcagcg ctgggaactc
      781 gatcacgacc tcaatcgttt cgagacccaa ttcgcgcgcg acggtcagcc tgtgatttcc
      841 atcgtgcagc ttgtagtgct tgccctggtg aacgaggccg attggcggca cgaggtgagg
      901 cgtttcacgg aaagcctttc gtagccattc gatgctcgca gggcgctcga cgtgcgtctt
      961 gatgtagctc agcgcgtcga cgcgcatccg ttgcttcggc aacttgatca tgagcgtcct
     1021 cccgggaatc cgtaccgctc tgccgcctgt gccatcgctt cgcgtgcgcg gcggtcggtc
     1081 cagcgcacgt tgatctcttt gaaaccgagg cggagcgccg ccaccacccg atgatggtcg
     1141 tccgggccgt cgccaacgat cgggataata tggccgtgct cgtcgcactt tgcgcccaca
     1201 gtgcgaagtg cggcactttg ggtgagacgt tcgagcacga ttaccgtcag cgggtcgcga
     1261 gtgagctcag cttcgcatcg acggatgtca tcgcaattga agttgcacgc cttggcctcg
     1321 aacgcgtgca tcgcgcgcac gtactttccg agcacgtcgt ttccgtcgat acgaagcagc
     1381 ggcatcgaag cctcaacgat accggccgat gttttctcgc cgtgccggcg ccgggcgtgc
     1441 gagcgcctcg ccgagcgccg ccggcacacc gcgcgcgagt cgtctttgcc gtgcgagctc
     1501 cttctggccg gccttcatgt cggcgagcac gcggcggttt cgctgcaccc gctcgagttc
     1561 agcgcgcact tcgccgaagt gcgtgtccat gtcgggcgtg ctcaagccag cctctgcgat
     1621 ggcctcgtat tcgtcgaggt cgctcaacat cagcgtttct tcgtgactca ggtcgagcgg
     1681 atagttcacg ccacacctcc gacggcaggc tcggtcgacg aagttggcag gagcgagcgc
     1741 acgaagtcgg ccgcgcgctt cacctgctgg tcccagggaa acttcgcgaa cggaggagct
     1801 acctgccgca tgtactcgac cgccttgagc acgtcgttcg cgccagggta gagcgcgcgc
     1861 aactgctcca aggtgatgct gttcttcggc atcggcgtga ccgccacgcg cggcggcgcg
     1921 acgctttgag cgtttgcgag gccgatgctg acgaccggct cgcaacgacg aaacgcagct
     1981 tggtattcag tctccgctgc ttcgagctcc tgttcgagcg cgtgggcttt gcgacagtgc
     2041 tgcggcaagt cggggcgcgc tgcgtatgct cgatcgcgca gcgcgaggat ctgatgcgca
     2101 gcgctgaagg ccgttttgct tcggcggcag atcgcgaact cttcgtcggt caaaccgcac
     2161 gggttttcgt tggtgctcat cgtcgggttt ccttcctggt ccggctcgag ccaagtgcgc
     2221 tcggccttat tctttttcgt gagctcgaga cacgccgccg cgtacgcgat ttgttcggcg
     2281 cgcgacaacg cagcgaaacg cgggtcgagc ttgaagtgct cgatcgcctg gtcgatggcg
     2341 tcaggcgcgg cgacgtcgac attcgtgccg gtcaagttga cggccggacg gtcgtacccg
     2401 ccctcttcgt cggccggctc ggtcgcgtcg ttcgcgacgg gcgggccgag cagcacgcgc
     2461 cgtgcagcga gcccgcgtgc cgacgccgcg atctgcaacg gcttcaaccc acgaaggaaa
     2521 gggtgattcg tgaacgcgat gctcgtcagc atcggcccga ccgacgcgcc cgaaacaccg
     2581 tccgtcccat cgagcgtgaa cgcgatcgac gtgaacctgt attcgttcgc cgcgatctgc
     2641 tggcggatct ggtcacccaa agccgcgagt gcccaaagct cggggccgtt cgagccgtcg
     2701 cgcacctcga gatccaacac ccacgccggc gccggggctc cgctcgtcgg gatcgagcct
     2761 tccgtcgcag agagctcgct cgcgtgttcg tagtcgaacg ggatagtcgg cttgtcgccc
     2821 acgccgtcgg gaccggcgtg gaactgcggg tgcgcgtg
//
LOCUS       DASTKS010001117         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1070451, whole genome shotgun sequence.
ACCESSION   DASTKS010001117 DASTKS010000000
VERSION     DASTKS010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070451"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFQ35_23595"
     CDS             <1..194
                     /locus_tag="VFQ35_23595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933717.1"
                     /translation="AGQLATSAEDYLLFDSACAGTRIDRYLSVSLQAGSYLVETASFK
                     PDRATFALVHRLRSHLKVA"
     gene            complement(227..853)
                     /locus_tag="VFQ35_23600"
     CDS             complement(227..853)
                     /locus_tag="VFQ35_23600"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HET9933718.1"
                     /translation="MPLTDITVRQARPKQKEYKLSDERGLLLVVRPTGAKWWRLRYQF
                     QGKEKMISLGVYPDVSLSMARDRRDAARRLVADGKDPSAERQAEKQALKLASEATFEK
                     IARHYLATLARKVRRGKGSIKTYKKAKWMLETFIFPKLGASPISQITASDLLVELKKI
                     EAKGMNETARRTKQRCALRKGVPIQRLPRAKQRFVMDMIDTVLAQQDR"
     gene            complement(1100..1184)
                     /locus_tag="VFQ35_23605"
     tRNA            complement(1100..1184)
                     /locus_tag="VFQ35_23605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1148..1150),aa:Leu,seq:cag)
     gene            1279..>2864
                     /locus_tag="VFQ35_23610"
     CDS             1279..>2864
                     /locus_tag="VFQ35_23610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HET9933719.1"
                     /translation="MNRRRSKYGRRDRGRGSSASSERSERRHAAESRAENGTRVTHAA
                     ESGARHAHSSARDRKHIKAIKAGRAAQSSRASGRASGAGRTGGARAGGVERVLSAGGV
                     VEGTVSAHRAGYGFLRVEGASDSVFLPPREMRGVMHGDRARVRVSRDASDRWFGAVEG
                     VVSRGVNAFLGTVEGHGRAAWVSAADRRLQLRCTVAPADLNGARDGDWVIAQLTRHST
                     GSQAPQARVQKRLDPDRPVELSTESAIARFELPHEFSAAALREAQAFGDKVDPNEIAQ
                     RADIRDLPLVTIDGEDARDFDDAVYAEPHPSGFRLVVAIADVSHYVRPGTALDAEAVE
                     RGTSVYFPTRVLPMLPHALSDHLCSLAPKVDRLCFVADMIVTKQGALKSATFYPATMR
                     SAYRLTYTLANEALFEGKRAAREQLGPLLDKLMVLVDVYRALYRARTRRGALDFDAAE
                     AEFVIDSAERVRAIELRVRNDAHRLIEECMILANVAVARELERAKMPTLYRVHAEPEE
                     QKIERLVSALASLNIDARLP"
BASE COUNT          497 a          898 c          958 g          511 t
ORIGIN      
        1 cggctggtca acttgctacg agtgccgagg attatctgtt gtttgactcc gcatgcgctg
       61 gaacacggat cgaccggtat ctaagcgtgt cgctgcaggc gggcagttac ttggttgaaa
      121 ctgccagctt caagcccgac cgagctacgt tcgcgttggt tcatcgcctg agatcccatc
      181 tcaaagtcgc gtgacgccgc cctgaacact gcggggcggc agcagctcag cgatcctgct
      241 gagcgagcac ggtatcgatc atatccatga cgaagcgctg cttggcgcga ggcagtcgct
      301 ggatgggaac acctttccgc agcgcgcagc gctgttttgt gcggcgggcc gtttcattca
      361 tgcctttcgc ttcgatcttc ttgagttcga cgagcagatc ggaagcggtg atctgggaga
      421 tcgggcttgc cccgagcttc gggaagatga acgtctccag catccacttc gccttcttgt
      481 aggtcttgat cgagcctttg ccgcggcgga ccttgcgtgc gagcgtcgcg aggtagtggc
      541 gcgcgatctt ttcgaacgtg gcttcgctgg caagcttcag cgcttgcttc tcggcctgac
      601 gttcggcgct cggatccttg ccgtcggcga ccaggcggcg ggccgcgtcc cgacgatccc
      661 gcgccataga caaggagacg tcggggtaga ccccgaggct gatcattttc tccttgcctt
      721 gaaactggta gcggagcctc caccatttgg ctccagtggg ccgcaccacc agtagaagcc
      781 cccgctcatc cgagagcttg tattctttct gtttgggccg tgcctgccgg accgtgatgt
      841 cggttagcgg catgacggca ctccgtggcg atggagccga ttcatcgggg caacctgcgc
      901 ctcaattccg aggttcgagt ccctgtttga aacggggcca aagttaccca gaaagttacc
      961 cagcttcgaa tcgtgctggg atgggcttcg gtggtacgcc ctggtacacg aaagacgaga
     1021 aaaaacttga agaaagaacc gcttacagct gcccctggga tggggcgaga cggcctggga
     1081 aggggtggga aggtcccact ggtgcccggg agaggactcg aacctccacg gagttacccg
     1141 ctagcacctg aagctagtgc gtctaccaat tccgccaccc gggcagggtg cgaaggcgcg
     1201 gcaatgtacg cagggggtcg agggctgtca attgactagc gtgtcggcag gcctgggtcg
     1261 tgcgcgtcga ggcatcggat gaacagacga cgaagcaaat acggtcggcg ggatcgtggg
     1321 cgcggctcat ccgcgagcag cgagcgcagt gaacgtcggc acgcagccga gtcgcgcgcc
     1381 gagaacggca cgcgcgtcac acatgccgcg gagtccggcg cgcgacatgc gcactcgagc
     1441 gcacgtgacc gcaagcacat caaggccatc aaagccggac gcgcagcgca aagctcgcga
     1501 gcgagtgggc gcgccagcgg tgcagggcga accggtggcg cacgcgccgg cggcgtcgag
     1561 cgtgtgctct ccgcgggcgg cgtcgtcgaa ggaacagtga gcgcacatcg cgcgggctac
     1621 ggctttctgc gagtcgaagg cgcgagcgac agcgtattcc tcccgccgcg tgagatgcgc
     1681 ggagtcatgc acggcgaccg tgctcgtgtg cgtgtcagtc gcgacgcgag tgatcgctgg
     1741 ttcggcgcag tcgaaggcgt cgtctcgcgc ggcgtcaacg catttctcgg gacggtcgag
     1801 gggcacggcc gcgcagcctg ggtcagcgct gcggacagac gcttgcagct ccgatgcacc
     1861 gttgcgccgg ccgatctgaa cggcgctcgc gacggcgatt gggtgatcgc gcaactcacg
     1921 cggcactcga ccggttcgca ggcgcctcaa gcgcgcgtgc agaagcggct ggatcctgac
     1981 agacccgtcg agttgtccac cgagtcggcc atcgcacgct tcgagctgcc gcatgagttc
     2041 tcggcggcgg cgctgcgcga ggcgcaagcc ttcggcgaca aggtcgatcc gaacgaaatc
     2101 gcgcagcgtg cggatatccg cgatcttccg ctcgtcacca tcgacggcga ggacgcgcgc
     2161 gacttcgacg acgccgttta cgcggagccg catccttccg gcttccgtct ggtcgtcgca
     2221 attgcggacg tgagtcacta cgtgcgtccc ggcacggcgc tcgacgccga ggccgtcgag
     2281 cgcggcacct ccgtgtactt cccgacgcgc gtgctgccga tgcttccgca cgcgctgtcc
     2341 gatcatctgt gttcgctcgc gccgaaggtc gatcggctgt gtttcgtcgc cgacatgatc
     2401 gtcacgaagc agggcgcgct gaaatccgcg acgttctacc cggcgacgat gcgttcagcc
     2461 taccgcctca cgtacacgct cgccaacgag gcgctgttcg aaggcaagcg ggccgcgcgc
     2521 gagcagctcg gtccgttgct ggacaagctc atggtgctcg tcgacgtgta tcgagcgctg
     2581 taccgagcgc gcacgcgccg cggcgcgctc gacttcgacg ccgccgaagc agaattcgtg
     2641 atcgactcgg ccgagcgtgt gcgcgcgatc gagcttcgcg tgcgcaacga cgcccaccgc
     2701 ctgatcgaag agtgcatgat cctcgcgaac gtcgccgtcg cgcgggagct cgaacgggca
     2761 aagatgccca cgctgtatcg cgtgcatgcc gagccggaag aacaaaagat cgagcggctg
     2821 gtcagcgcgc tcgcctcgct gaacatcgac gcgcgcttgc cgga
//
LOCUS       DASTKS010001118         4378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1965845, whole genome shotgun sequence.
ACCESSION   DASTKS010001118 DASTKS010000000
VERSION     DASTKS010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965845"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VFQ35_23615"
     CDS             <1..1378
                     /locus_tag="VFQ35_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HET9933720.1"
                     /translation="NGGTLAGGASPGGGAGAAASSGKAGAGGAGDPDAPASITKEGEG
                     VLTSSGLTIVSYGGYLNGESFQQEGIVTYKGYQYTAFWNANRNVVMARRALPSGAWEK
                     FDFTDYKNAEDDAHNTISLGVCPGDGTLHLSFDHHGSDLHYRRSMPGFLDSPNASTWN
                     ASSFTSVAKALVGTTTVAQVTYPRFVPEPGGKKMLFEARIGSSGSGDQYLWEYDSTTH
                     AWTSLGKYIDGIANNINAYPHGVSYTRNGTRLHVSWCWRETPDANTNHDLVYVYSDDN
                     GRTWNGTDGTAIGTSGSKAITLASGVPAVRIAQNRGLINQEHMAVDSQGRVHVLLSHM
                     PSSQPDDSNFERSRTKSEFFDYWRDTDGVWHTVALGYSVVANFRGKLAISSSNNVYAI
                     LPDLRIAGATSKSNFQTFALLDKSEAGRFFSDPLIDSSRLLSEDKLTIVYPQKSSPNI
                     YALDYALR"
     gene            1757..3292
                     /locus_tag="VFQ35_23620"
     CDS             1757..3292
                     /locus_tag="VFQ35_23620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933721.1"
                     /translation="MSHSRPPFRAVAGYVIGERLASGGMGSIHLGIKTGALGFQRVVA
                     IKRLHPELATDPEFSARFKDEIRLTGGLTHTNIVQTLDVVEAADELMLVMECVEGETL
                     AVLLGAARARGTVLALDLVSAVVTQALHGLHAAHESMDDAGRRLAMIHRDVSPHNIMI
                     NKDGVVKVLDFGIAKAALESHVTRAGHVCGKLAYMSPEQLRGRPLDRRVDVFSAGVVL
                     WESLSGQRLFGDLSLSDAAAVQNVLLKQVVPPSRFRSDVSPALERVVMRALAREPDQR
                     FGTALEFAVALNAACPSAPPAALAQALTEYCGPRLERRAQLAANFRRELKSSTTSAPV
                     AFAREPSDAPADSRESETRDVGGVVVLDDERRTSSLRQRWLLGASVALSVVVAALGLR
                     AFSSHRKPEPRVVSTSAARPEVVPVVPPSAPVEFEVASVVERPLTIEQLPAEGASTQP
                     IVEPPVTSPHAPIDTKPKRLPRARPSAEPTAPATPPPAEPQCSPPTYTDADGIRHFKP
                     GCI"
     gene            3292..4152
                     /locus_tag="VFQ35_23625"
     CDS             3292..4152
                     /locus_tag="VFQ35_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933722.1"
                     /translation="MVRTTTATCLLALMVTLSHRALADGGKAECIAAYESGQRARKSG
                     ALSKAKQAFEYCASESCPSAMHDDCSSWLHEVDDAIPTRAFRVTDGNGEPLPSATLSI
                     DGGEAQELSEEALSFEPGSHELTFECDGYAKVQRRLVLRPGEHAAPLDIELEPLAEVT
                     AAETAPEAPPRPKRFRDSSSPNWIPTLIGAGVGVLGTASFTYFGLRARSLDRSLDECI
                     PNCSAGRVADVRHNYTAANASLGIAAAGLLAAGVSWLLQPSSSSSSASSPGVRVSLRV
                     GPVTSLMGQF"
     gene            4149..>4378
                     /locus_tag="VFQ35_23630"
     CDS             4149..>4378
                     /locus_tag="VFQ35_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933723.1"
                     /translation="MKSRLVASALLVGLAVLACSPLHDLDAASRGSSGALGRGGASGS
                     SGGTSSGGSGVSLELLCKDGALNGAETDVDCGG"
BASE COUNT          761 a         1414 c         1421 g          782 t
ORIGIN      
        1 caacggcggg actctcgcgg gcggcgcgag cccgggtgga ggcgcaggtg ctgctgcttc
       61 gtctggaaag gcgggggctg ggggcgcggg cgacccggac gcgccagcgt cgatcaccaa
      121 agagggcgaa ggtgtgctca cgtcctcggg cctgaccatc gtctcttacg gcgggtacct
      181 gaacggcgag tcgtttcagc aagaggggat agtcacctac aagggttatc agtacaccgc
      241 gttttggaat gcgaaccgca acgtggtgat ggcgcggcgc gccctcccga gcggcgcgtg
      301 ggaaaagttc gacttcacgg actataaaaa cgccgaggac gacgcgcaca acacaatctc
      361 gctcggcgtt tgcccggggg atggcacgct gcacctctcg ttcgatcacc acggcagcga
      421 cttacactac cgtcgctcga tgccaggctt tttggacagc ccgaacgcat cgacgtggaa
      481 cgcgagcagc ttcacgtccg tggcgaaggc gctcgtcggg accacgaccg tagcgcaggt
      541 gacttatccg cgcttcgtcc ccgagccggg ggggaagaag atgctcttcg aggcgcgtat
      601 cggtagcagc ggcagtggcg accagtactt gtgggagtac gactccacga ctcacgcttg
      661 gacgtcgctc ggaaaataca tcgacgggat tgccaacaac atcaacgcat acccgcacgg
      721 cgtgtcctac acccgcaacg ggacgcggct gcacgtctct tggtgctggc gagagacgcc
      781 ggacgcaaac acgaaccacg acctcgtcta cgtttacagc gacgacaacg ggaggacgtg
      841 gaacggcact gacggtaccg caatcgggac gagcggctcc aaggcgatca cgctggcgag
      901 cggcgtgccc gcggttcgaa tcgctcaaaa tcgcggcctc atcaatcaag agcacatggc
      961 cgtcgactcg caaggtcgcg tacacgtact cctctcacac atgccgagct ctcaacccga
     1021 cgattcgaac ttcgagcgct ctcgcacgaa gagcgaattc ttcgactatt ggcgtgacac
     1081 ggacggcgtt tggcacacgg ttgcactcgg gtacagcgtg gttgcgaatt ttcggggaaa
     1141 gctcgcgatt tcgtcgagca acaacgtcta tgccattttg ccggatctgc ggatcgccgg
     1201 cgcgacgtcg aagtcgaatt ttcagacgtt cgcgctgttg gacaagtccg aggccggacg
     1261 cttcttctcc gacccgctca tcgattcttc acggttgctg agcgaagaca agctcacgat
     1321 cgtttatcca cagaagagct cacccaacat ctacgcgctc gactacgcgc tgcgctagca
     1381 gtgccattgc ttcgcgttcg agtcgtggac ggcacgcgcg gcgccgatga aatcggagag
     1441 actcatctcg cgagcggcga cacactattc gagcgctctg tagccgcgtg tcggcgtcga
     1501 gcggttacaa tcgcctgttc acccgctcgg gtatgttccg gaccgagaca cacccgcttt
     1561 gggatcttgt gaaacatgtt gcgaacgcga cccccgtgcg cgaaaaattc gaatttgcgg
     1621 atccgttaac gagtttcgaa caactcaacg cattgagctg cgccgtttca atcatcccga
     1681 gccactacag tctacggtgg ccccgagcca ggcaactcgc ccagtggtcc gtacaggcaa
     1741 cgaacgtacc cagccaatgt cgcactcacg cccacctttc cgtgccgtcg ccgggtacgt
     1801 gattggagag cgtcttgctt caggtggcat gggatcgatc cacctcggca tcaagacggg
     1861 cgcgctcggg tttcagcggg tcgtcgccat caagcggcta cacccagagc tcgcgacgga
     1921 cccggagttc tccgcgcgct tcaaggacga gatccggctc accggagggc tgacgcacac
     1981 caacatcgtc cagacgctgg acgtcgtcga agccgcggac gagttgatgc tcgtgatgga
     2041 gtgcgtcgaa ggcgagacgc tcgcggtgct gttgggggca gcgcgagctc gcggcaccgt
     2101 gctcgcgctc gatctcgtga gcgcggtggt gactcaagcc ctgcacggct tgcacgcggc
     2161 acacgagtcc atggacgacg cggggcgtcg gctggcgatg atccatcgcg acgtctcgcc
     2221 ccacaacatc atgatcaaca aggacggcgt cgtgaaggtg ctcgacttcg gcatcgccaa
     2281 ggccgcgctc gagtcgcacg tcacgcgcgc cgggcacgtt tgcggcaagc tcgcctacat
     2341 gtcgccagag cagctgcgcg gccgtcctct cgatcgtcgc gtggacgtgt tctcggcggg
     2401 cgtggtgctc tgggagtcct tgagtgggca gcgcctgttc ggtgacttga gcctgtccga
     2461 cgccgcggcg gtgcagaacg ttctcttgaa gcaggtggtt ccaccgagcc ggtttcggtc
     2521 ggatgtgagt ccggcgctgg aacgggtggt gatgcgcgct ctcgctcgag agcccgacca
     2581 gcggtttggg acggcgctcg aatttgccgt cgctctgaac gccgcgtgcc cttcagcccc
     2641 tcctgccgcg ctcgcccaag cgctcacgga atactgcggg ccgcgcctcg agcgccgcgc
     2701 gcagttggcg gcgaactttc gtagagagct gaaatcgtcg acgacgagcg cgccggtcgc
     2761 gttcgcgcgc gagcccagcg atgcgcccgc ggattcgcga gagtccgaga cgcgcgacgt
     2821 cggcggcgtc gtggtgctcg acgacgaacg tcggacgtct tcgcttcgtc agcgctggtt
     2881 gctcggcgcg agcgtcgctt tgtcggtggt cgtggcggcg cttgggcttc gcgccttttc
     2941 atcgcaccgg aagccggagc cgcgcgtggt gtccacgtcc gcggcgcgtc ccgaagtcgt
     3001 tcccgtagtt cccccctcgg ctccggtcga gttcgaagtg gcgtccgtcg tggagcgccc
     3061 gctcacgatc gagcaactgc ccgccgaagg cgcttcgacg cagccgatcg tcgaaccccc
     3121 ggtgacgtcg ccgcacgccc ccatcgatac gaaacccaag cgcctgccgc gcgcgcgccc
     3181 gagtgccgag ccaaccgctc cggcaacacc accgccagcg gagccgcagt gttctccgcc
     3241 gacatatacc gatgccgacg gcatccgtca cttcaaacca ggatgcattt gatggttcgg
     3301 acgacgacgg cgacttgcct gctggctctg atggttacgt tgagccaccg tgcgctggcc
     3361 gatggcggta aggccgaatg catcgcggcg tacgagagcg ggcagcgcgc tcgaaagagc
     3421 ggcgcgctgt cgaaggccaa gcaagctttc gaatattgcg cgagcgagtc gtgcccgagc
     3481 gcgatgcacg acgactgctc gagctggttg cacgaggtcg acgacgccat tcccacgcga
     3541 gcctttcggg taacggacgg caacggtgag ccgctgccaa gcgcaaccct gtccatcgac
     3601 ggcggagaag cgcaggagct ttcggaggag gcgctctctt tcgaacccgg ctcccacgag
     3661 ctcaccttcg agtgcgatgg ctacgccaag gttcaaaggc gtctggtact tcgcccgggc
     3721 gagcacgctg cgcctctcga catcgagcta gagccactcg ccgaggtcac cgctgccgaa
     3781 actgcgccgg aagcacctcc ccgccccaag cgtttccggg attcgtcgag cccgaattgg
     3841 atccccacgc tgatcggcgc gggcgtcggg gtgctcggca ccgcaagctt cacctacttc
     3901 ggtctcaggg cgcggagcct cgatcgttcg ctcgacgagt gtattccgaa ctgcagcgca
     3961 gggcgtgtgg ccgacgtgcg tcacaactac accgccgcga acgcttcact cggaatcgcg
     4021 gccgcaggcc tgctcgcggc cggagtatcc tggctgctgc agccttcgtc ctcctcctcg
     4081 agcgcctcgt cgccgggtgt tcgcgtgtcg ctacgcgtag ggcccgtcac gtcactgatg
     4141 gggcaatttt gaagagtcgc ctcgtcgcca gcgccctcct ggttgggctc gcggtgctcg
     4201 cgtgttcacc gctgcacgat ctcgatgcgg cttcgcgcgg gagctcgggg gcgctcggtc
     4261 ggggcggtgc ttcggggtcc tcgggcggga cttccagcgg cggtagcggt gtttcgttgg
     4321 agcttctgtg caaagacggt gcgctgaacg gcgccgagac cgacgtcgac tgcggagg
//
LOCUS       DASTKS010001119         8855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1295437, whole genome shotgun sequence.
ACCESSION   DASTKS010001119 DASTKS010000000
VERSION     DASTKS010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295437"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFQ35_23635"
     CDS             <1..270
                     /locus_tag="VFQ35_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933724.1"
                     /translation="VIELSGPTDLTPRDVAAAFSKLLGKPVQVVEAPLDAVVPTFTSF
                     GASENIASLFREMYEGLALGRVASEPGEHRRGTTPVEVTLRALLG"
     gene            521..1672
                     /locus_tag="VFQ35_23640"
     CDS             521..1672
                     /locus_tag="VFQ35_23640"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9933725.1"
                     /translation="MRTTRVLALLPALLAAPVRADEAVFKIGMSTVLSGPAQGLGQGM
                     RAGVEAYFDRVNKSGGIAGKKLELVALDDQYEPSKAGPNVRRLIDDEHVLAVIGNVGT
                     PTAVVTVPIVNEKKVPLFGAFTGAGVLRKNPPDRYVFNVRASYGDETAEMVRGLLDDR
                     HLAPSDIAFFTQDDAYGDAGYSGAAKALEARGFKDAERLPHGRYTRNTLDVEDALARL
                     LDPRVHPKAVIMVGTYKPCAKFIRLARKNGLKAVFANVSFVGSAELLKELGKEAEGVV
                     ITQVVPHWSSSLPILSEYREAGLTPSFVSLEGFLVGRAFSEVLRAAGANATAESFVQA
                     AESGRAFDIGLGTPQALSGTRHGFSSQVWPTVIQRGEFRAFSNWKDVTP"
     gene            1669..4332
                     /locus_tag="VFQ35_23645"
     CDS             1669..4332
                     /locus_tag="VFQ35_23645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933726.1"
                     /translation="MKSPRSIERNLLSFGLALASLIVVLVIALFAVEHALGNRIERLG
                     TATVPAAQAATNLRHAVSRVFERQVQVLSTRTESELAPLAERSAIERALGESRQRLSS
                     TLKGIAPADAAERETARLSASIDEVLTRDASLFDSVRVRHGAEAKFEARAQASKASLD
                     QLIQEARAVAGLGHLEYVLELRRVAGGASPELVLRGNARAQQEAAEQVVTAVLQLGQL
                     MGKIALAKDQDELNSITANELAQNLSRARTQLKVLVLSLEGAADTAERARKMQSQFDA
                     VAAQISAEKDAQSLVSLRRSILVEASHASALRAKILDSVKRLSGELDLVERIVADETS
                     DSMRAARRALWVTRTLSLLFVFAAVAVGFHAARRLRDSVRGLRAQNQELASLSRDLKQ
                     MNEGLEALVAERSAALVARERSMRLVLDAMSEGLVLADLSGNLVGESSKASISWFGAA
                     PEGTPIWDYLLAHDVGKREAFRVGFAQIAEDLLPFEVSVDCMPRRFELNGRVFSLGYR
                     QVREDDVFQRVLVVVKDISEQVAGEERERDAREQHQLLAHLLKDKQGFLSFVRDAERL
                     LATLRGEITRDGALRTLHTLKGNTGVFGMESVARRCHELEERLTEREDGITETEAKEL
                     VELWRARLSRVEGALTRENAIELYESDLVELVSGLRGRRDYPQLIELVESWKWTRTSV
                     LLRRLAAQVHRVGERLNKHVEVRIEHNNLRVMPGALDDFWGSLIHVIRNAVDHGIETE
                     AERAQQGKPAHGIITLRTTQLGEGFAVELADDGRGIAFDDLRRIAEARGLPSKTRRDL
                     LDVMFSDGVSTREQATDTSGRGVGLGAVVAACRAAGGMVDVDSGSGKGTSFKFAFPKQ
                     AVEVRVGASSGTFHTVRPRSLRAPSNARNAE"
     gene            4477..6114
                     /locus_tag="VFQ35_23650"
     CDS             4477..6114
                     /locus_tag="VFQ35_23650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933727.1"
                     /translation="MSKCSFARVLSGLALVLLAGCSADGGGKGEPGPTPEVEGASFEL
                     SVDATKLPIVQGSTAELAVEVVRKNGFEGAVVVTASGLPAGAAMTSVTIPEGETEASL
                     VFSALPDAPHSLPSAVTLRGSSTAIQKELALTVTVCGLPGALDTSFEGGRVVVPVGAG
                     EDYAYAIAAQSDGKLLVAGSSAENQGDFALVRLQRDGALDSSFGQGGKVTTPIGAGTD
                     VARAIAIQSDGKILLAGSSVGTGTGTDFALARYTSDGRLDTSFGDGGKVVTALSNDAD
                     VAYALMLQKDGKIVLGGQVNNGASATGLDFALVRYDSNGTLDATFGTQGHVFSALAQN
                     SGSDVVYSLVGQMVEGEERLVAAGGEGDFALARFTASGALDATFGANGKVVGLLGSVI
                     GAARAVRVTAQGELLVAGHKAHDFALARLTEDGELDATFGTKGITITPITENWDEAQA
                     LQIDAEGKIVLGGWAYEGSSSSGNFALARYSPLGQLDQSFGTGGIAVTPVASGTKDDA
                     ANAVLLSMDDRVPTVRILLAGSANGSNHDFAVTRYWR"
     gene            complement(6237..7031)
                     /locus_tag="VFQ35_23655"
     CDS             complement(6237..7031)
                     /locus_tag="VFQ35_23655"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET9933728.1"
                     /translation="MKDNVVSGIRSHRYASTSAVAVGLALVLSAVGCERKQGNDMSQT
                     APPSENPITPGGAPGLVPRISPAKPGPEVTTDTPGAPGAQMTGDRNGAGQPTAAGDRT
                     GASEPMAAGEQANTPRKAEAKFKVASGYKLSGKAKLEEVANGVKVVVDLDDAPPGKKG
                     IHVHEKPDCSDIPNKSMGEHFAPGAKAHGLPPNAARHLGDLGNIDIDKNGDAHFEFVA
                     QNANLKPGDPMSFLNRAIVIHESSDKGAQPSGNSGKPIACAVIEKD"
     gene            complement(7240..7674)
                     /locus_tag="VFQ35_23660"
     CDS             complement(7240..7674)
                     /locus_tag="VFQ35_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9933729.1"
                     /translation="MANTSGRADSSVANTTSNPNVTNSKSAESSPDTSVTKVDSRYSP
                     HGAMGQAYLATGVTLGMRLWRNEPVNEAKPSVRREYEVVGYVLAGRAELEIEGQKVLL
                     NPGDSYVVPRGASHHYRILESFSAVEATSPPAQAHGRDEPTH"
     gene            8212..8610
                     /locus_tag="VFQ35_23665"
     CDS             8212..8610
                     /locus_tag="VFQ35_23665"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET9933730.1"
                     /translation="MFLWNDSYKVGVEEIDRQHEKLFSLLRDLHDAVTVGKGRTVVKQ
                     VLDGVVAYTKEHFATEERLMQKYDYADFAAHKHMHDSLVAQAHQLQEKTQHSALTLSM
                     EVMKFLESWIAHHISEADRKLGHYLSSKGV"
BASE COUNT         1497 a         2772 c         3007 g         1579 t
ORIGIN      
        1 gtgatcgagc tctcggggcc gaccgatctc acgccgcgcg atgtagcggc cgcgttttcg
       61 aagctgctcg ggaagcccgt gcaggtggtc gaagcgccgc tcgacgcggt cgtcccgacg
      121 ttcacctcct tcggagcgtc cgagaacatc gccagcctgt ttcgcgaaat gtacgagggc
      181 ctcgcgctcg gcagagtcgc ctccgagcct ggggaacatc ggcgcggcac gaccccggtg
      241 gaagtgacgt tgcgcgcgct gctggggtga gcgacgagcc tgcgctctca tgcgcgagtc
      301 ccgacctcgt cggcgaaggg gcgcgtcccg acacacgccc ttgcggcgag gtcgcgcgag
      361 ctcgtttcat tccgagacga gactggcgct tacatggcgg gccgttgcgg atcgtcatga
      421 gttacgcggt ccgtacgaga gcggccttac cccagtagcg agttggtcgt tccgcaccgg
      481 ttagcccgtc cgaggaacta cgggcactgc cggattcgcc atgcggacta ctcgtgttct
      541 cgcgctcctc cccgctcttc ttgccgcgcc ggtgcgcgcg gacgaagcgg tcttcaaaat
      601 aggtatgtcg accgtgctgt ctgggccggc ccagggccta gggcagggca tgcgcgccgg
      661 ggtcgaggcg tatttcgacc gagtcaacaa gtcgggtgga atcgccggca agaagctcga
      721 gctcgtcgcg ctcgatgacc agtacgagcc gagcaaggcc gggcccaacg tgcggcgcct
      781 gatcgacgac gagcacgtgc tcgcggtgat cggcaatgta gggacgccga cggccgtggt
      841 gaccgtcccg atcgtgaacg agaagaaggt gcccttgttc ggcgccttca cgggagcggg
      901 ggtgctgcga aagaaccctc ccgaccgcta cgtcttcaac gtgcgcgcga gctatggcga
      961 cgaaacggcc gagatggtgc gcggcctgct cgacgaccgc catctcgccc cgagcgacat
     1021 cgcatttttc acccaggacg acgcttacgg cgacgcgggg tattcgggtg cggcgaaggc
     1081 gctcgaggcg cgcggcttca aggacgccga gcgtctcccg catggccgct atacgcgcaa
     1141 cacgctcgac gtcgaggacg cgctcgcgcg gttgctcgat ccgcgcgttc accccaaggc
     1201 cgtcatcatg gtcggcactt acaagccgtg cgccaagttc atccgattgg cgcggaagaa
     1261 cggcctgaag gcggtgtttg cgaatgtatc gttcgtgggc agcgccgagc tcttgaagga
     1321 gctcggcaaa gaggcggaag gcgtggtcat cactcaagtg gtgccgcact ggtcttcgtc
     1381 gctgccgatc ctgagtgaat accgggaggc tgggctcact ccgagcttcg tttcgctcga
     1441 aggcttcttg gtgggccgcg ccttcagcga ggtgctgcgc gccgcgggcg ccaacgccac
     1501 cgccgaaagc ttcgttcaag cggcggagtc ggggcgcgcc ttcgatatcg gcctcgggac
     1561 gccgcaagcg ctctcgggaa cccgtcacgg cttcagctcc caggtttggc cgaccgtcat
     1621 tcaacgcggg gaatttcgag ctttctcgaa ctggaaggac gttacgccat gaagtcgccg
     1681 cgctccatcg agcgaaactt gctgtcgttc ggcctcgctc tggcgtcgct gatcgtagtg
     1741 ctggtgatag cgctgttcgc agtcgagcat gccctcggca atcgcatcga gcgcctcggg
     1801 accgcgaccg ttcccgcggc ccaggccgct accaatttgc ggcacgccgt ctcgcgggtc
     1861 ttcgagcggc aggtgcaggt gctttcgacg cgcaccgaga gcgagctcgc ccccctcgcc
     1921 gagcgctccg ccatcgagcg cgcgctcggt gagtcgcggc aacgcctgag ctcgactctg
     1981 aaggggatcg ctcccgcaga cgcggccgag cgagagacgg cacgcctctc cgcgagcatc
     2041 gacgaggttc tgactcgcga cgcttcgctc ttcgacagtg ttcgcgttcg ccacggcgcc
     2101 gaagcgaagt tcgaggcccg cgctcaggct tccaaggcat cgctcgatca gctgattcag
     2161 gaggcgcgcg ccgtcgcagg actcgggcac ctcgaatacg tactcgagct ccggcgtgtc
     2221 gccggcggcg cctcccccga gctcgtttta cgcggtaacg cgcgagccca gcaggaggcg
     2281 gcagagcaag tggtgaccgc ggttctgcag ctcggccagc tgatggggaa gatcgcgctc
     2341 gcaaaggatc aggacgagct caactcgatc accgccaacg agctcgccca aaacctgtcg
     2401 cgagctcgta cccagctcaa agtgctggtc ttgtcgctcg aaggcgcggc cgatacggcc
     2461 gagcgcgccc ggaagatgca gtcgcagttc gacgcggtgg cggcacaaat cagcgccgaa
     2521 aaggacgccc agagcctggt aagcctccgt cgcagcatcc tggtcgaagc ttcgcacgcg
     2581 agcgcgctcc gcgcgaagat tttggacagc gtgaagcggc tttcgggtga gctcgacctg
     2641 gtcgagcgca tcgtcgccga cgagaccagc gactcgatgc gggcggcgcg ccgggccttg
     2701 tgggtgacgc gcactctctc gctgctcttc gtgttcgccg cggtcgccgt tggctttcac
     2761 gccgctcggc gtttgcgcga cagcgtgcgc ggccttcgag cccaaaacca ggagcttgcg
     2821 tccttgagtc gggacttgaa gcagatgaac gagggcctcg aagcgctcgt cgcggagcgt
     2881 tcggccgcgc tcgtcgcgcg tgaacgtagc atgcggctcg tgctcgacgc gatgagcgaa
     2941 gggctggtgc ttgcggatct ttcgggaaac ctggttggag agtcctcgaa ggcgtccatt
     3001 tcctggttcg gagctgcccc cgaggggacg ccgatctggg attacttgct ggcccacgat
     3061 gtgggtaaac gcgaggcgtt tcgagtgggg ttcgcccaga tagcggaaga cctgctgccg
     3121 ttcgaggtgt cggtagactg catgccgcgg cgcttcgagc tgaacgggcg cgtcttttcg
     3181 ctcggttatc gccaggtgcg ggaggacgac gttttccagc gcgtgctcgt ggtcgtgaag
     3241 gacatcagcg agcaagtcgc aggcgaggaa cgtgagcgcg acgcgcgcga gcagcatcag
     3301 ctcctcgcgc atctcctgaa agacaagcag ggcttcttgt cgttcgtgcg tgacgcagag
     3361 cgcctgctcg cgacgctgcg cggcgagatc acgcgggacg gcgcgctgcg cacgctgcac
     3421 accttgaaag ggaacacggg cgtgttcggg atggagagcg tggcgcgccg ctgtcacgag
     3481 ctcgaggagc gcctgaccga gcgggaagat ggcatcacgg agaccgaggc caaagagctc
     3541 gtagagctct ggcgcgcgcg gctgagtcgg gtcgaagggg cgctcacgcg cgaaaacgcc
     3601 atcgagcttt acgagtcgga tctggtcgag ctggtctcgg gcctacgggg ccggcgagac
     3661 tatccgcagc tcatcgagct cgtcgagtcg tggaagtgga cgcgaacctc ggtgctgttg
     3721 cgtcgcttgg ccgcgcaagt ccaccgggtc ggtgagcgcc tgaacaagca cgtcgaagtg
     3781 cgtatcgagc acaacaacct gcgcgtgatg ccgggagcgc tcgacgactt ctggggctcg
     3841 ctcattcacg tgatccgcaa tgcggtcgac cacggcatcg agaccgaggc cgagcgcgca
     3901 cagcagggca agccggcgca cggtatcatc acgcttcgta cgacccagct cggcgaaggc
     3961 ttcgcggtgg agctcgcgga cgacggccgc ggcatcgcct tcgacgacct acggaggata
     4021 gccgaggcgc gcggcttgcc gagcaagaca cggcgcgatc tcctggacgt gatgttctcg
     4081 gacggggtga gcacccgcga gcaggccacc gatacgtccg gacgcggtgt cgggctcggc
     4141 gccgtggtcg cggcctgccg cgccgcgggg ggcatggtcg acgtcgattc gggctccggt
     4201 aagggaacct cgttcaaatt cgcgttcccg aagcaagccg tcgaagtgcg ggtcggcgcg
     4261 agcagcggaa cctttcacac cgtccggccg aggtcgttgc gcgcacccag caacgcccgc
     4321 aatgcggagt aggcttgtaa tcggctgtaa ttcagcgggc ggcgattagg cggccgaaat
     4381 cgtgcgcgcc ctgtaacggc ggcgcgagtc gcaccacttc agcgggcgag agccgagagt
     4441 ctctctcggc tcgttcgtcc aaggagttgt gtgctcatgt cgaagtgttc gttcgctcgc
     4501 gtcctttctg gtctcgctct ggtgctgctc gctggatgct ctgccgacgg cggaggcaaa
     4561 ggggagccag ggcccacgcc ggaggtcgaa ggagcgagct tcgagttgtc ggtcgatgct
     4621 acgaaactcc ccatcgtaca aggctctacc gccgagctcg cggtcgaggt ggtgcggaag
     4681 aacggtttcg agggcgcagt ggtcgtgacc gcatcgggct tgccagccgg agcggcaatg
     4741 acctccgtga caatacccga gggtgagacg gaggctagcc tcgtgttcag cgctctcccg
     4801 gatgcgccgc attctttgcc cagcgctgtc acgctgcgcg gtagttccac cgccatccag
     4861 aaggagctcg cgctgaccgt cacggtgtgc gggctgccgg gcgcgctcga cacgagcttc
     4921 gaaggcgggc gcgtcgtagt gccggttggg gcgggtgaag actacgccta cgcgatcgcg
     4981 gcgcagagcg acggaaagct cttggttgcg ggcagcagcg ccgaaaacca aggagacttt
     5041 gcgctggtgc gtttgcagcg cgacggcgct ctggactcga gcttcggtca aggtggaaag
     5101 gtgacgaccc caatcggcgc tggcaccgac gtggcgcgcg ccatcgccat ccaaagtgac
     5161 ggcaaaatct tgttggctgg ctcgagcgtc ggaaccggca cgggaaccga ttttgcgctg
     5221 gcgcgctata cgagcgacgg gcgtttggac acttcgtttg gcgacggagg caaggtcgtt
     5281 acggctctat cgaatgacgc cgacgttgcc tacgcgctaa tgctccaaaa ggacggcaag
     5341 atcgtgctgg gtggccaggt gaacaacggc gcgagcgcaa cggggctcga ctttgcgctc
     5401 gttcgctacg actccaacgg cactttggat gccaccttcg gcacccaggg tcacgtcttc
     5461 agcgcgctcg ctcagaattc gggaagcgac gtcgtctact cactcgtcgg tcagatggta
     5521 gagggcgaag agcgcctggt cgccgccggg ggcgagggcg acttcgcgtt ggcgcgcttt
     5581 acggcgagcg gcgcgctcga cgcgactttc ggcgccaacg gcaaagtggt cggcctcttg
     5641 ggctccgtca tcggcgcggc ccgcgcggtg cgggtgacag cgcagggcga gctcctcgtc
     5701 gccggccaca aagcgcacga cttcgccttg gcgcgcctca ccgaggacgg cgagctcgac
     5761 gcgaccttcg gcacgaaagg catcacgatc acgcccatta ccgagaactg ggacgaagcc
     5821 caagcgcttc aaatcgacgc cgagggcaaa atcgtgctcg gcggctgggc ctacgaaggt
     5881 tcgagctcga gcggcaactt cgcgctggct cggtacagcc ccctcggcca actcgatcaa
     5941 tccttcggca cgggcgggat cgccgttacg ccggtcgctt ccggaaccaa ggacgacgcg
     6001 gcgaacgcgg tgctcctatc gatggatgat cgcgtgccca cggttcgcat cttgctcgcc
     6061 ggatctgcga acggctcgaa ccacgacttc gccgtgacgc gctattggcg gtgacacgcg
     6121 ctgagacgct cgcgcttttg cggcgaaagt aaacgggcgc tcgctgggga tagcaagcgc
     6181 ccgttcggcg ggcgtggtct tcgaaaccaa acccgctcag ctctatgcga agatgctcag
     6241 tctttttcga tcacggcgca cgcgatcggc ttgccggagt ttccggaagg ctgggctccc
     6301 ttgtcgctgc tctcgtgaat cacgatcgcg cgattgagga acgacatcgg atcgccaggc
     6361 ttcaaattcg cgttctgggc gacgaactcg aagtgcgcgt cgccgttctt gtcgatgtcg
     6421 atgttaccga ggtcgcccaa gtggcgcgcc gcgttcgggg gcagcccgtg agcctttgcc
     6481 cccggagcga agtgctcccc catgctcttg ttgggaatgt cgctgcagtc cggcttctcg
     6541 tgaacgtgaa tgcccttctt tccaggagga gcatcgtcca agtcgaccac gactttcacg
     6601 ccgttcgcta cctcttcgag cttcgctttc ccggagagtt tgtacccgct cgcgaccttg
     6661 aatttggctt cggctttgcg cggagtgttc gcttgctcac cggcggccat gggttcactc
     6721 gcaccggttc gatccccggc cgcggtcggt tgccccgcgc cgttgcggtc ccccgtcatc
     6781 tgcgctccgg gcgctccggg cgtgtcggtg gtgacctcgg gtccgggctt tgcggggctg
     6841 atgcgcggca cgaggccagg cgctccgccc ggcgtgatcg ggttttcgct cggcggcgcg
     6901 gtctgcgaca tgtcattgcc ttgcttgcgc tcacagccga cggcagagag cacgagggcg
     6961 agcccaaccg cgaccgccga ggtcgaagcg tagcgatgcg aacgaatccc ggaaacgacg
     7021 ttgtccttca tggagactcc tcgttgatgc caagtaactg cgtatctgca cccaagctcg
     7081 cttgggtgga cggaaccaag tcagaccgtt cgtgtgggcc gagcaccggc tcgagccgtt
     7141 gtgcgcggca cgcttgtcat tgcgcggcgg cgttgctcct cgcaggcgaa atgcgacgta
     7201 cggtttgaca ccgagtcttc tcggataccc tcgatgcggt cagtgcgtcg gctcgtcgcg
     7261 tccgtgagct tgcgcgggtg gcgacgtcgc ctccaccgcc gaaaacgact cgagaatgcg
     7321 gtagtggtgc gaagcgccgc gcgggaccac gtacgagtcg cccggattca agaggacctt
     7381 ttgcccctct atctcgagtt cggctcgccc agcgaggacg tatccgacga cttcgtattc
     7441 gcgacgaacc gagggctttg cctcattcac cggttcgtta cgccagagcc gcatgccgag
     7501 cgtcacgccc gtggcgagat aagcttgtcc catcgccccg tgtggcgagt atcgagaatc
     7561 gactttcgtc acgctcgtgt ccgggctcga ctcggcgctc ttcgagttcg tgacgttcgg
     7621 gttcgacgtg gtgttcgcga cggacgagtc cgcgcggccg ctcgtgtttg ccatcgagct
     7681 ccaccaagca tcaacggtgc caagcgcgac ttgcggtcct cgccggcggc accccggcaa
     7741 tcgtgtgtcc gtccgaacgg cgccggtgtc ggcaaagcgc cacgctgagg cgagacaggt
     7801 gtcggcgcaa acccgttcac cggcaaggcg atagaggaag ggtcggggct cgcgaccgag
     7861 cgcggtggag cctactaatg acgagacgtg atgaactcac gtaggcgatg gaccgcggtg
     7921 cgaggtctcg caaactgcaa cgcaagagct gcacgctcgc gtggccacgc agcgaatccg
     7981 aacgcttgct cgaggtagaa cctgcgcgac gcattccgtt cgctgcgcgg cgctggacta
     8041 cggatcgcgg cggctccgcc gcgcgagttc gtgtcgtgca tcgcggtagt catgcgcgcc
     8101 gttcccggtc agtgaatgcg accgggcgag tgaccgcgcg ctcaattctt cgcgaacgcg
     8161 gccgctggaa tggactgagc ggagaaccta cgctccgcga cgagggccaa cgtgttcttg
     8221 tggaacgata gctacaaagt gggtgtcgag gagatcgatc gacagcacga gaagttgttc
     8281 tcgttgctgc gcgacctgca cgacgcggtc accgtgggca aaggtcgaac ggtggtgaag
     8341 caagtgctcg acggcgtggt ggcgtacacg aaggagcact ttgcgaccga agagcggctg
     8401 atgcagaagt acgactacgc ggattttgcc gctcacaagc acatgcacga ttcgctggtc
     8461 gcgcaggcac atcagctgca ggaaaagacc cagcacagcg cgctcacact ttcgatggag
     8521 gtgatgaagt tcctcgaatc gtggatcgcg catcacatca gcgaagcgga ccgaaagctc
     8581 gggcactatt tgtccagcaa gggtgtttga gctcgctcga gtggcgcgtt ctccgccgag
     8641 gtcctaaccg cgcacgctgc gagggggctg atggatcgct ggcgaacaga gtgcgcgcac
     8701 cgtgtcgtac tcacgcctcg atctgggtac taaagcgaca tccgcttgct gaagaaccct
     8761 aagtttcgcg cggtttccgt cgttttgctc agcggagagc cagagaagga cggagcggcc
     8821 cacgaaggcc tccgcccctc cccgactgag ccggg
//
LOCUS       DASTKS010001120         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_847052, whole genome shotgun sequence.
ACCESSION   DASTKS010001120 DASTKS010000000
VERSION     DASTKS010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847052"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFQ35_23670"
     CDS             <1..557
                     /locus_tag="VFQ35_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933731.1"
                     /translation="SGLGNAEVVHRETDSVGGFSGRYEARFEMMKRSGDWSRAHPWFE
                     CFLSPSRAASMIRRALELLPPFFGDGHRMFAIKVDERLEFLSMPEAEGGFVIAFAVLP
                     TAIPEWALGDALGALARLQDLVQSEGGKRYLSGWLGSPGPEFWKTHYGARYERWVNEK
                     LRHDPSGVFSSAMFAPLDAAVYAR"
     gene            687..2384
                     /locus_tag="VFQ35_23675"
     CDS             687..2384
                     /locus_tag="VFQ35_23675"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9933732.1"
                     /translation="MDEPLDTTIWIETPEHIQFRYRVAGPSRRALAYALDFSLRAVIG
                     GVLAVLFGLGGASGFQLGAVLLMLFLLEWGYFVVCELATGGRSPGKRALDLRVVTLSG
                     APISFGDSVLRNLLRAADFLPSAYVFGLLAMLFDARFRRLGDLAAGTMVVYEKRQRLR
                     PPIDVEPPKARELARLPERVDLASESVLAIEQFLRKVPDLSEQRAEELAEILAEPHAA
                     RLRLKYKSAVRFLEILHYVAVQGRTVQDREAFISSRSGAWRELEELLATRRSLSELRP
                     ADISRAAALYRSACADLMHARLIGCPADVIGFLDALTARAHSALYSSRSRAFATVRDL
                     LLSRFPRAFRRQGWFMAASAALFLVPLAAGWVGAWMSPGFAQKVLPPHQLEALARAYA
                     EGFSFGRSENLDAAMAGFYVENNIGIAFRCFATGILLGLGSIFFLLYNGLAIGTTFGY
                     VVRAGAGANILTFGCGHAPFELTAIVIAGGAGLRLGFALVDTDGLTRLGSLRKHSLDL
                     VALVSGAAVMLAIAALIEGFWSPSSMPAHFKYAFSAVGVAVMSAFLGFASREPEART"
BASE COUNT          340 a          785 c          835 g          521 t
ORIGIN      
        1 tttcagggct cggtaacgcc gaagtggttc atcgagaaac ggattcggtt ggcggattct
       61 cgggccgcta cgaggcacgc ttcgagatga tgaagcggag cggagactgg tcgcgcgcgc
      121 acccgtggtt cgagtgtttt ctgagtccga gccgggccgc ttccatgatt cggcgcgcac
      181 tcgagctctt gcccccgttc ttcggcgatg gacaccgtat gttcgccatc aaagtcgacg
      241 agaggctcga gtttctctcg atgcccgaag ccgagggagg gttcgtgata gccttcgccg
      301 tgcttccgac tgcgatccca gaatgggcgc tgggcgacgc gctgggcgct ctcgcgcggc
      361 tccaggacct cgtccaaagc gagggtggga agcgttacct atctggttgg ctcggaagcc
      421 ctggccccga attctggaaa acgcactacg gagctcgtta cgagcgctgg gtgaatgaga
      481 agttgcggca cgatccgagc ggcgtcttct cttcggcaat gtttgcgccg ctagacgcgg
      541 ccgtctacgc gcgctgagcg ctctcgccgg tgttcggccc ttgacgcctc tgagcgggag
      601 gggtggagtc tgactccccg atgggagtgg atgtgcgagc gactgcatcc gagggcgtgc
      661 aacgcgcgcc gtcgacggcg gatgtcgtgg acgagccgct cgacacgacc atttggatcg
      721 agacgccaga gcacattcag tttcgctatc gagtcgccgg gccttcacgt cgcgcgctcg
      781 cgtacgcttt ggacttctcg ctgcgcgcgg tgattggcgg ggtgctcgcg gtgttgttcg
      841 gcctcggcgg cgcgtcgggt tttcagctcg gggccgtcct cctcatgttg tttctgctcg
      901 agtggggcta cttcgtcgtc tgtgagcttg ccacgggcgg acgaagcccg ggaaagcgcg
      961 cgctcgattt gcgcgtggtc acgctgagcg gagcgcccat cagcttcggc gatagcgttt
     1021 tgcgcaacct gctgcgagcc gccgatttcc ttccgagcgc gtatgtcttc ggcctcttgg
     1081 ccatgctgtt cgacgcgcgg tttcgccgac tcggggatct cgcggcgggc accatggtcg
     1141 tgtacgaaaa gcggcagcgt ctgcgccccc ctatcgatgt cgagccgccg aaagcgcggg
     1201 aactcgcgcg cctgccggag cgggtggatc tggcgagcga gagcgtcctc gcgattgagc
     1261 agtttctgcg caaggtcccc gacctcagcg agcagcgcgc ggaggagctc gccgagatct
     1321 tggcggagcc tcacgcggct cgtcttcgct tgaaatacaa gagcgcggtg cgcttcctcg
     1381 agattctcca ttacgtcgcc gttcagggac gcaccgtgca ggatcgtgag gcgttcatca
     1441 gctcacgcag cggcgcgtgg cgagagctcg aggagctgtt ggcgactcgc cgtagcctct
     1501 cggagcttcg acccgccgac atctcgcgcg cggccgcgct gtaccgctcg gcgtgcgccg
     1561 acctgatgca cgcccgtctc atcggttgcc ccgcggacgt catcgggttc ctcgacgcgc
     1621 tcacggcgcg cgctcacagc gccctctact cgtcgcgttc gcgagcgttc gcgaccgtgc
     1681 gcgacctctt gctctcgcgc ttcccccggg ctttccgcag gcagggctgg ttcatggccg
     1741 cgagcgcggc gttgttcttg gtcccgctcg cagcgggctg ggttggcgcc tggatgtcgc
     1801 ctgggttcgc gcaaaaggtc ttgcctccgc atcagctcga ggcgttggcg cgggcgtacg
     1861 cggagggctt ctccttcggt cgttcggaga acctggacgc tgcgatggcc ggattctacg
     1921 tcgagaacaa catcgggatt gctttccgtt gtttcgcgac tggcatcctg ctcggcctcg
     1981 gcagcatctt cttcttgctc tacaacggcc tcgcgattgg cacgacgttc ggctacgtcg
     2041 tgcgcgctgg tgccggcgcg aacattctca ccttcggttg cggacacgcg ccgttcgagc
     2101 tcactgcgat cgtcatcgca ggcggcgccg gcttgcgcct aggcttcgcg ctcgtcgaca
     2161 ccgacgggct cacccgactg gggtcgctcc gaaagcactc gctcgatctg gttgcgctcg
     2221 tgtctggcgc cgccgtcatg ctcgccatcg ccgcgctgat cgaaggattt tggtcgccgt
     2281 cgagcatgcc tgcacacttc aagtacgcgt tcagcgccgt cggcgtcgcc gtgatgtcgg
     2341 ctttcttggg gtttgcgtcg agggagccgg aggcgcgcac gtgaacctcg gccaaactcg
     2401 aattgccgtg cgtgagcgca acaccgctga aatcatcgat ttgggctgcc gcgtgccttt
     2461 ttctctgggg ggcgagctgt a
//
LOCUS       DASTKS010001121         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_401125, whole genome shotgun sequence.
ACCESSION   DASTKS010001121 DASTKS010000000
VERSION     DASTKS010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401125"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFQ35_23680"
     CDS             <1..1174
                     /locus_tag="VFQ35_23680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9933733.1"
                     /translation="LNPEDVVSGMHTVYEGSLESDSTDGDEPTEDELVVEDEVVAPKA
                     ETPPSNESRFASLPPRARTDSSFPKSMHGVVGMSETLVDLYRIVDRVADTTCTILITG
                     ESGTGKEIVARAVHRASPRSEAPFVAVNCGAIPEALLESELFGHARGAFTGAHQAKPG
                     RIAMAQGGTLFLDEIGEMPLALQVKLLRILQSREYSPVGDNRVLKADVRIVAATNINL
                     ESAVQAGTFREDLYYRLNVIHVHVPALRERAEDIPLLFHHFLARAKEKLGRPQIMGLS
                     RTAAQLLAEYHWPGNVRELENTIERAVLLCSGNCIEPRDLPQRVLGLGAEKRIAARLP
                     DTGIDLRNAVEAFENQLIRQALERTQWNKKQAAGLLGLNRTTLVEMLKRKRITRAA"
     gene            1393..1875
                     /locus_tag="VFQ35_23685"
     CDS             1393..1875
                     /locus_tag="VFQ35_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933734.1"
                     /translation="MSAVLWLAPAEARAEGWMFDGVAGIGSGIEGGVPGSADIAWRRA
                     RTRVIAGFDMRSDEAEKDVFGLRAFAEIERRGGVGGELRYERWLGRWLSGYVFATGTV
                     VPETLVGAGFGASLVIPFGKRAGFAIEPSFAALPLGTDVPDNSVILWASLSLNLKVGL
                     "
     gene            1872..2339
                     /locus_tag="VFQ35_23690"
     CDS             1872..2339
                     /locus_tag="VFQ35_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933735.1"
                     /translation="MKRNAFTSLLTSALLALSVGVDACRGGAQCLRNSDCAVGERCQL
                     GACVMESSPESGAGDAGEDGSAGQHAGGGSSAVGKGGSGGESTNQGGKNNGGAAGASG
                     SAGRGGTAGNAGAAGRGGSAGSAGRGGSSSGTARGGSAGSTARGGANSSGGSN"
     gene            2392..>2696
                     /locus_tag="VFQ35_23695"
     CDS             2392..>2696
                     /locus_tag="VFQ35_23695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HET9933736.1"
                     /translation="MFRSLHIAATGMAAQETQLESISNNISNGNTVGYKKQRVDFQDL
                     LYQTIRAPGAPTGTTTTSPTGLQLGSGVRVVSTSRVFSQGTVLTTNNPLDIAIEGDG"
BASE COUNT          460 a          887 c          910 g          439 t
ORIGIN      
        1 actcaacccc gaagacgtcg tctcgggcat gcacaccgtt tacgagggaa gcctcgaaag
       61 cgactccact gacggcgatg agccgaccga ggacgagctg gttgtcgagg atgaggtcgt
      121 cgctccgaag gccgagactc ctcccagcaa cgagtcgcga ttcgcgagcc tcccgccgcg
      181 cgcacgcacc gactcgagct tcccgaagag catgcacggc gtcgtcggga tgtccgagac
      241 gctggtggac ctctatcgca tcgtcgatcg cgttgccgac accacctgta cgattctgat
      301 cacgggcgag agcggcaccg gcaaagagat cgtcgcccgc gcggttcatc gagcgagtcc
      361 ccgcagtgaa gcgccgttcg tcgcggtcaa ctgcggcgcc atcccagaag cgttgctcga
      421 gagcgagctg ttcggccatg cgcgcggcgc cttcaccggc gcgcatcagg ccaagccggg
      481 tcgtatcgcg atggctcagg gcggcacgct cttcctcgac gaaatcggtg agatgccgct
      541 cgctctgcag gtgaagctct tgcggatcct gcagtcgcgc gagtactcgc ccgttggcga
      601 caaccgcgtc ctcaaagccg acgttcgcat cgtcgcggcg acgaacatca acctcgagag
      661 cgccgtccaa gccggcacgt tccgcgaaga cctctactat cgcctcaacg tcatccacgt
      721 ccacgtcccc gctctgcgcg agcgcgccga agacatcccc ctgcttttcc accactttct
      781 cgcgcgtgcg aaggagaagc tcggtcgccc tcagatcatg gggctttcgc gcacggcggc
      841 gcagctgctc gccgagtatc actggccggg aaacgtgcgc gagctcgaga acaccatcga
      901 gcgcgcggtg ctcctctgtt ccggcaactg tatcgagccg cgtgacctgc cccagcgtgt
      961 gctcggactc ggcgccgaga agcgcatcgc cgcgcgcctc cccgatactg gcatcgatct
     1021 gcgcaacgcc gtcgaagctt tcgagaatca gctcatccgc caagcgctcg agcgcacgca
     1081 gtggaacaaa aagcaagccg ctggtttgct cggcttgaac cgcacgacgc tggtcgagat
     1141 gctgaagcga aagcgcatca cgcgcgcggc ctaagattcc cttccgttct cattctgttc
     1201 tcctttatcg aacctcctgc tcgcctcgcg tttccggccg gcgtcgtcaa ttcgggcgac
     1261 gtcggccgga gacgcgtgta ggcactccgt ccctcgagta gcgactcgaa ccgagcacgg
     1321 actcgcgctg gcccagcgct tgctctagtc ggcgtcagat tgactctgcg ccgtcttgcc
     1381 atctgggccc cattgtctgc cgtgctgtgg ctcgcaccag cggaggcgcg cgccgagggc
     1441 tggatgttcg acggcgtcgc aggaattggc agcggtatcg agggcggggt gccgggctcg
     1501 gcggacatcg cttggcggcg cgcgcggacc cgagtcatcg cgggattcga catgcgctcg
     1561 gacgaagccg aaaaggatgt gttcggtttg cgcgcgttcg cggaaatcga gcgacgcggc
     1621 ggcgtgggcg gcgaactccg ctacgagcgg tggctcgggc gctggctgtc gggatacgtg
     1681 ttcgcgaccg gcaccgtggt gcccgagact ctagtcgggg cgggcttcgg agcgagcctg
     1741 gtcattccgt tcggcaagcg cgcgggcttc gccatcgagc cctccttcgc cgcgctaccg
     1801 ctcggaaccg atgtgcccga caacagcgtg atcttgtggg cgtcgttgtc cctgaatctc
     1861 aaggtagggc tatgaagcga aatgcgttca cctcgctgct cacgagcgct ctgctcgcgc
     1921 tgagcgtcgg tgtcgacgct tgtcgcggag gcgcgcagtg cctgcgcaac agcgactgcg
     1981 cggtgggtga gcgctgtcaa ctcggcgcgt gcgtcatgga atcgtcgcca gagagtggag
     2041 ccggtgacgc gggcgaggac ggctcggcgg gccagcacgc gggcggaggc agcagcgccg
     2101 tcggcaaagg cggctccggg ggcgagtcga cgaaccaagg cggcaagaac aacggcggcg
     2161 ccgcgggcgc gtctggcagc gcaggacgcg gcggaaccgc gggcaatgcg ggcgcggcgg
     2221 gacgcggcgg cagcgccggc agcgccggtc gtggcggaag ctcgagcggc accgcgcgcg
     2281 gcgggagcgc cggcagcacc gctcggggcg gcgcgaactc gagcggcggc agcaactgag
     2341 cggcgagccg aggcttggca ctcgcgttgc agtgctgagt cgcggagcca catgtttcga
     2401 tccctacata tcgccgcgac cgggatggct gctcaagaga cgcagctcga gagtatttcc
     2461 aacaatatct cgaacgggaa caccgtcggg tacaagaagc agcgcgtcga ctttcaggat
     2521 ctgctctatc agacgatccg agcgccgggc gcgccgacag ggacgaccac gacgtcaccg
     2581 acgggcctac agctcggctc tggcgtgcgc gtcgtgagta cgtcgcgagt gttctcgcaa
     2641 ggcacggtgc tcaccaccaa caatccgctc gatatcgcaa tcgaaggcga cggctt
//
LOCUS       DASTKS010001122         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1072059, whole genome shotgun sequence.
ACCESSION   DASTKS010001122 DASTKS010000000
VERSION     DASTKS010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072059"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFQ35_23700"
     CDS             complement(<1..1015)
                     /locus_tag="VFQ35_23700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9933737.1"
                     /translation="MTALLELNGYGVAFGERVVLRDIDLGLPALGMTVLMGPAGGGKS
                     TFVRTLAGLNDLHPTLRVFGSARFGGRALSENSRVDERPLLLAQHGRFFLSTVRENLV
                     SALPDRSRLSQLEQTALVRRHLEQLGMQALVESLALDAVSLPLATQRKLLLARALLQA
                     PRALFADESTVGMDDASAAEFVELLVRVAEERLVLFVTHNQRLARMAGGSALLLAFGA
                     IQESAPTHTFLSAPRTDAGRQFVRTGGCSSASTPDSASTPDSAPERPPVPVTASAPRG
                     FFWVTKGRLGGSPRPGIVEELRTDLAGLRELGVTRVVTLEERRSVGAAELVEFGMRGF
                     HFPI"
     gene            complement(1012..1329)
                     /locus_tag="VFQ35_23705"
     CDS             complement(1012..1329)
                     /locus_tag="VFQ35_23705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933738.1"
                     /translation="MFALVNRRQELVQHDSALLKSVLADLHERDSAIVDEALVERIHA
                     LEGLDAELDALLARKAERPREEFLRVLERLATPYRERDEEGRTPTRLSLITNAPPSRR
                     SAL"
     gene            complement(1314..1970)
                     /locus_tag="VFQ35_23710"
     CDS             complement(1314..1970)
                     /locus_tag="VFQ35_23710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933739.1"
                     /translation="MSGLQQPVLELLQAGRTRLAQILEDVERLSEGWSSELGLLEEPP
                     EETVARAVDPVNAIGEIVFVASLELRLSEERLRSAGATSATTLLAECDSALRHLAKGL
                     RAVVHAASRSREEPRVSAHVELAVSRQVRRAYARFRRRIAAAEAAPDLHSRFRAAGTQ
                     IAMLVGHEAYPRMRVRDRLVLQDLQRRILAWLRDGGIDAKAGEQLCKISPRASRCSRS
                     "
     gene            complement(2374..3276)
                     /locus_tag="VFQ35_23715"
     CDS             complement(2374..3276)
                     /locus_tag="VFQ35_23715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9933740.1"
                     /translation="MGESSRVDWQAVARLLAERVSRAAVVLDAQGTIRLLNAEMEGLL
                     ERERFYVEGQPWAALFDVNSTHSSEWLTRALDGSLRMHHAEAKVSPTRSVQFEFDVAQ
                     VGTGATPALLLVESFHQISDSMQPASADAPLEYEIEASVDSFGTLVGVTRQGKPVQGN
                     VGRHCFRLFHSRDIPCEECPVRLPNSVPWPRTSIRHRVGEGTFEVVQAEWGEHNRARL
                     RIISIAETTLQAIHRSKLKALSVKGELSERESEVLQHLLSGRSVEEIAELLAVAPRTV
                     KYHQANLLAKLGADSRIDLLRLLF"
     gene            3833..>4690
                     /locus_tag="VFQ35_23720"
     CDS             3833..>4690
                     /locus_tag="VFQ35_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9933741.1"
                     /translation="MTSSHDSREGESSRARIETLAIHAGQSPDPVHGAVMPPIVLSST
                     FAQPEPGKPLRYDYSRSGNPTRESLESCLAALEGAKFGFAFASGCAAASTLLHTLGPE
                     DHVVCGDDVYGGTYRIFARVLEPLGIRTSFVDLRDTRKLEAAIRDRTRLVWLETPTNP
                     MLRLADIEAVSTITRARGLKLVVDNTFASPMLQRPLELGADVVLHSTTKYVNGHSDVV
                     GGALLTSDAELAQRLKFLQNAIGAVPSPMDCYLVLRGIKTLPLRMQRHVETASELAQR
                     LEVEPGVLRV"
BASE COUNT          871 a         1595 c         1458 g          766 t
ORIGIN      
        1 gaatcgggaa atggaagccc cgcatgccga actcgacgag ctccgccgcc ccgacgctcc
       61 ggcgctcctc caaagtgacg acccgggtca cccccagctc gcgcaaccct gccagatcgg
      121 ttcgcagctc ttcgacgatg ccgggccggg gagatccgcc gagccgaccc ttggtcaccc
      181 agaagaagcc gcgcggagcg ctcgccgtca cgggaacggg cgggcgctct ggagctgaat
      241 cgggcgtgct agccgaatcg ggcgtgctcg cgctcgaaca gccgcccgtg cgcacgaact
      301 gtcgcccggc gtccgtacgg ggcgcggaca aaaacgtgtg cgtcggtgcg ctttcttgaa
      361 tcgcaccgaa agcgagtagc aatgcgcttc cccccgccat gcgcgcgagc cgctggttgt
      421 gcgtcacgaa gagcaccaac cgctcctcgg cgacgcggac gagcaactcg acgaactcgg
      481 cggcactcgc gtcgtccatg cccaccgtcg actcgtctgc gaacagggcc cttggcgcct
      541 gcagcagcgc tcgggcgagg agcagttttc gctgcgtcgc gagcggaagc gacaccgcgt
      601 cgagcgccag actctccacc aaggcctgca tcccgagctg ctcgaggtga cgccgcacga
      661 gcgccgtctg ctcgagctga gagagccgac tccgatcagg cagagctgac accaagttct
      721 cgcgcaccgt gctcaagaag aagcgaccgt gctgggcgag caggagcggg cgctcgtcca
      781 cacgcgagtt ctccgagagc gcccgtccgc caaagcgcgc gctaccgaag acacgcagcg
      841 tcgggtgcaa atcgttcaac cccgccagcg tacgcacgaa cgtcgatttg cctccgccgg
      901 cggggcccat cagcacggtc atgccgagcg ccggcaaccc gaggtcgata tcgcggagta
      961 cgacgcgctc gccaaaagcg acaccgtaac cgttgagctc gagcaacgcc gtcatagagc
     1021 gctccggcgg gaaggcggcg cgttcgtaat caggctcaag cgcgtcggtg ttcggccttc
     1081 ctcgtcgcgc tctcgatatg gcgtggcgag ccgctcgagc acccgcagaa attcctcacg
     1141 tggacgctcg gcttttcgcg cgaggagggc gtcgagctcc gcgtccaaac cttcgagcgc
     1201 gtgaattcgc tcgacgagcg cctcgtccac aattgccgag tcgcgttcgt gaaggtcggc
     1261 gagcaccgac ttcaacagcg cggagtcgtg ctgcaccagc tcctggcgcc ggttcacgag
     1321 cgcgaacatc gcgatgcacg cggtgagatc ttgcaaagct gctctccggc ttttgcgtcg
     1381 atgccgccat cgcggagcca ggcgaggatt cggcgctgta agtcctgcaa gacgaggcgg
     1441 tctctcaccc gcatgcgcgg gtaggcttcg tgaccgacca acatcgcgat ttgcgtacca
     1501 gctgcgcgaa accgggaatg caggtcgggc gcggcctcgg cggccgcgat acggcgacga
     1561 aacctagcgt aggcccgacg cacctgccgt gagaccgcga gctccacatg tgcgctcacg
     1621 cgcggctcct cccgagagcg agacgccgca tgaaccaccg cgcgcagccc tttggcgaga
     1681 tgccgcagcg ccgagtcgca ctcggcgagc agcgtggtcg cgctcgtcgc gcccgcggac
     1741 ctcaagcgct cttcgctcaa ccggagctcg agcgacgcga cgaacacgat ctcacctatc
     1801 gcgttgactg ggtccaccgc ccgcgcgacg gtttcctccg gcggctcctc gagcaagccg
     1861 agctctgaag accacccctc gctcaacctc tcgacatcct ccagaatttg cgcgagacgg
     1921 gtgcgaccgg cttggagcag ctcgagcact ggctgctgga gtccactcac gacggtcacc
     1981 gcgtccgcac gctcgaacga gcgaacatcg aggcgcgcgg cgcgctctag taatgccccg
     2041 aattcaaggc atgcagaagc gacaatcgcc gcgtccgtcg ctcgggatga gtaagaagcc
     2101 accatgccac ccgactcgcg gcgcgcgcag caccatcatg tgccagaacg cccgagtcat
     2161 actgtgtacc cccgtgctcg acgtgttcga ctcgagcagt cgggcttgcg aaggcgaccg
     2221 cgccttgtcg cgaacacaga ccttaaacga ccactcctga tgttgaaact gtccggagtg
     2281 acctagaacc tgttccagaa gtcaggatcc gacgtcggtt cggggcgcac agtggcaatc
     2341 acgaaccgac tccaaacaaa cacacatggc gtgtcagaac aagagacgaa gtagatcgat
     2401 acgcgagtcg gcgccgagct tcgccagcaa gtttgcctgg tgatacttga cggtgcgcgg
     2461 cgctaccgca agcaattcgg cgatctcttc gacgctgcgt ccgctcagta ggtgttgcaa
     2521 cacctcggac tcgcgctccg agagctcacc cttcacgctg agcgccttca acttcgagcg
     2581 atgaatcgcc tggagcgtcg tttcggcgat ggagatgatg cggaggcgcg cgcggttgtg
     2641 ctcaccccac tcggcctgca cgacctcgaa cgtcccctca ccgacgcgat gacggatgga
     2701 ggtgcgaggc caaggaacgg agttcggcaa acgcaccgga cattcttcac aggggatgtc
     2761 acgcgagtga aaaagtcgga aacaatggcg acccacattg ccctgaacgg gcttaccttg
     2821 gcgtgtcacg ccaacgagtg ttccaaagct atctacgctc gcctcgatct cgtactcgag
     2881 cggagcgtct gcgctggccg gctgcatcga gtcgctgatt tggtgaaagc tctcgacgag
     2941 cagcagggcc ggggtggcac cggttccgac ttgggcgaca tcgaactcga actgaacgga
     3001 gcgagtcggt gaaaccttcg cctccgcgtg atgcatgcgg agcgagccgt cgagagcgcg
     3061 cgtcagccac tcactcgaat gggtggaatt cacgtcgaac agcgcggccc agggctggcc
     3121 ttcgacgtag aatcgctccc gttcgagcag cccttccatc tccgcgttca gaagacgaat
     3181 cgtgccctgt gcgtccagca ccaccgccgc gcgactgacg cgctccgcaa ggagacgcgc
     3241 taccgcttgc caatcgacgc ggctactttc gcccatctat tcgcccgacc caatttccgc
     3301 tgcccacgac acgaaccatt cgcacaggat tctgtcaggt tcggtaccgt ctgcgtcaag
     3361 cgccgagcgc ctgcctttta gaacaggttg actgtcttcg tggacagagc catcccatca
     3421 cgcgataggt ttcttagtat ttcggcgctg gaaatcgagc gcggcactcg aggacatctc
     3481 gagcgtcgat tcatcgcgga gcgggcaacg cgtcagcgaa gagctcgcca cggaggggtg
     3541 acgtgacgct aggtgcgctt gccgagagcg acgttcgttg gaaacgcgag gtgagcggtc
     3601 ctcggccgca cgtgccctcg acgtaccgga cgcgcggcca cgattgctcg ctcccagatc
     3661 gcggtggcaa cgcgatcgtc ggtaccccgc cgcgctcatc tcgcgagtgc gaacgtcggt
     3721 ggtcgatgct cccacctcgt cctggccgcc cgcccggcgc ctgttcgcgg ctgcggcccg
     3781 cgcgccggcc ccatctcttg accaggttgg tcactgggcg aaggcttctc cgatgacgtc
     3841 gagtcacgac tcgcgcgagg gtgagagctc acgtgcacgc atcgaaacat tggcgattca
     3901 cgcagggcaa tcgccagacc cagtgcacgg cgcggtgatg ccgcccatcg tgctgtcgag
     3961 caccttcgct cagcccgagc ccggcaagcc actgcgttac gactattcga ggagcggcaa
     4021 cccgacgcgc gagagcctcg agtcctgcct cgcggcgctc gaaggcgcaa aattcggttt
     4081 cgcgttcgcg agcggctgtg cagcggcaag tacgttgttg cacacgctcg gccccgaaga
     4141 ccacgtcgtc tgcggcgacg atgtttatgg ggggacgtac cgcatcttcg cgcgggtgct
     4201 cgagccgctc ggcattcgca cgagcttcgt cgaccttcgc gacacgagga agctcgaggc
     4261 cgcgattcgc gaccgaacgc gcctcgtctg gctcgagacc cccaccaatc cgatgctgcg
     4321 cctcgccgac atcgaagccg tctcgactat tacgcgggca cgcggcctga agctcgtggt
     4381 ggacaacacc ttcgcatctc cgatgctgca gcggccgctc gagctcggcg ccgacgtcgt
     4441 tctgcactcc acgaccaagt acgtcaacgg ccactccgac gtggtcggcg gtgcgctact
     4501 cacctccgac gcagagctcg cgcagcggct caagttcctg caaaacgcga tcggcgcggt
     4561 cccgagcccg atggattgct acttggtact acgcggcatc aagaccctgc cgctcagaat
     4621 gcagcgccac gtggagaccg cgagcgaact cgcgcagcga ctcgaagtcg agcccggcgt
     4681 gttgcgagtg
//
LOCUS       DASTKS010001123         7863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1520597, whole genome shotgun sequence.
ACCESSION   DASTKS010001123 DASTKS010000000
VERSION     DASTKS010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520597"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFQ35_23725"
     CDS             <1..402
                     /locus_tag="VFQ35_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933742.1"
                     /translation="DGGQNRPCANNPSGHLDLSQPGASAAGVQGNPDLKNQAKWQFVP
                     CPVNGNVIVRLKQGNNNEFFIENEILPIQSVTCEGQNGSRTSYGAWHFDQNVNGKPCT
                     VTDISNRSIDITVGSTQNENVDTGVQFPKCN"
     gene            737..1399
                     /locus_tag="VFQ35_23730"
     CDS             737..1399
                     /locus_tag="VFQ35_23730"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9933743.1"
                     /translation="MQKKTNRPELQTVSARNRAAWRRWLERHHAKARGVVLVYAKKGS
                     AEASIDWAESVDEALCFGWVDGVRGKLDETHFTVRFTPRKSGSLWSKRNLARIAHLTE
                     AGLMHPAGLRAFELGKARGAHERAYAIREEVAMPPELDAELRKRDQARAAFEALARGQ
                     KKGWYRWIAGAKREATRMQRARDALLLIAAGRKAGETDNQAARRGVPTKARILGRTRR
                     DS"
     gene            complement(1406..3139)
                     /locus_tag="VFQ35_23735"
     CDS             complement(1406..3139)
                     /locus_tag="VFQ35_23735"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933744.1"
                     /translation="MKGARRSLLRGLAPYFRPHWRQLFAVAAYLLVKLATDIVIPWST
                     KVLIDEVIPSRDVARLGTWSALVFLSFSLGSLAAYRWSVVSGALAVRILAELRARCIE
                     KVHALSFRYHNETASGDLLARLTGDVDRLQGLVERVLPALLFEGASLVVITVVALRLN
                     VLLAAVVLGLGAPVFVALYFATNRRLGEASRELQDREGRLTALASEQIQNQRIVKLFG
                     LERWAHESFARLLASTQTAATAVVRLSALLNGGTNFVFHGVRFVVLALGAALVVRGSM
                     STGAVVAFFTLVGGLIAPFVTIAEQFVELGVGAGAFARVQALLTAKEELQFGSVTLAP
                     PAREIRLVQACVRHGDNPALVDVSLSIPAGALVALVGPSGSGKSTLLGLLARLYDPDS
                     GAVRWDELDLRDATRDSLRSRIAVVPQTPYLFCISVLENARLGRLTASEEDVRRALFD
                     AGLGDGSELGDRGFDALAGEQGSHVSGGQAQRVAVARALIREADVLLLDEATAALDAR
                     AELILLEHLDWLKKRGTTIVFATHRPAAASRADWVFTFERGRLVRSQSREEFARRTLD
                     ATGGLESDLRA"
     gene            complement(3136..6405)
                     /locus_tag="VFQ35_23740"
     CDS             complement(3136..6405)
                     /locus_tag="VFQ35_23740"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HET9933745.1"
                     /translation="MSSAAKPKATSAVASMEQARGSYEASGCFVLRTPLLPVSEWTSL
                     NAGLSAPAHAADSSELAAALQSDHALVSERLARLWKRGELREAVFLASPTLTRSVDRW
                     CAEKEREPAAAQSWLRYFTRMATRPTPFGLFAGYSTGSVGEVTALELAARSEYRRRTR
                     LDMECVSAIASALESSLSRRGLRYRANSSLYEAAGRLRYVEDRRDGRRRTYHLVAVES
                     SEALATTLERARTGATLGELAAALVGPGVSKEDADGFLAALVERRILVSELEPALTGD
                     DALEQLVRALEELGADDLVTVLRAVGDDLARFDRAVAGNDQGGYAATAERIVPISPAA
                     ADLRALFQVDLEKPCGQSTLGSGVCRELERAVHLLHRISAPRPDALADFKRRFSERYE
                     EQEVPLVEALDAESGIGFGGEALALHEDSPLLAELPFPPVSSEHGEDFGRRDVALMRL
                     LERVWRDSKLVLELADSDIEALAANSPPPLPDALAVLATVLDSTSAAEPRLLIHSATG
                     PSGARLLGRFCARDPRLTALVRAHVGAEEALRPSAVFAEIVCHPQDRVGNVLARPLLR
                     RYEIPYLGRSGAPAPAQIPVTDLWVRVVGERVVLRSRKLGCEVLPRLSTAHFYGDPRN
                     PSVYRFLGALQSQGVSAGLSFSFGPFEQASFLPRVTYQNLVLSRARWNVSAEELQPIR
                     ELTGAARFAALRALRARRSLPRHVVLAEDDNELPLDLENVVACEALLERPRERVALYE
                     LLTSAESCKGPEGLFEHELIVPFVRRGSASTPAQAAASKPTRPALERSERTFCPGSEW
                     LYAKVYGGEASLEGVLTRAIAPLRRAVRASGAADSWFFLRYSDPDPHLRVRFHGEPSR
                     LSAEVLPAVHAALAPFVGSRSIVRVEIGTYEREVERYGGLAGVRVAERHFDLDSDAAL
                     EVVERALDGGGDAERFELTIAGMDALFQGFSLDLRARSELATRLRDAFALEFRADVAL
                     KEAFRERFRGERARLEALLSRGATSDSESPEYRYADWIAHRKAQAARIAEELRGLESA
                     GQLECPMLDLVPIFAHLFVNRTMRSTQRAHEYLFYELLSAHYRSALGRARAHGRVERE
                     Q"
     gene            complement(6402..7673)
                     /locus_tag="VFQ35_23745"
     CDS             complement(6402..7673)
                     /locus_tag="VFQ35_23745"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HET9933746.1"
                     /translation="MHLLEPDQEQAAWRAIVAIARDLAALAPSRSSGAREASLASGDA
                     GIALFFEMLSRTERGEGIGASALADEYLGAALDALGEQSLDPSLFAGFSGVAWVFALL
                     CRERGEEVGSDLDAVDEALAQYVARNASAELDLITGSVGIGAYLLERLPRPLAQEGIA
                     EIVRALERAAEERDGLVTWRTPANALDDADRRLYPEGRYDLGVAHGVPGTIAFLARVC
                     AAGIEKARSERLLEGALAWLARQATKGSPSPRFPYWKAAVGPSPATRLSWCYGDLGIA
                     LALLSAATAIRRTDLWDEAVELAHAVADRAPESAGIVDAGLCHGTAGAAHQFHRLHEA
                     TREPKFSRVSRFWFEQTLAARREGEGVGGFRAVVRDANLNDVWLDEPEFLNGAAGIGL
                     ALLSALTPDEPFWDRALLISPLASERPDPPR"
BASE COUNT         1473 a         2654 c         2587 g         1149 t
ORIGIN      
        1 gatggcggac agaaccgccc gtgcgcgaac aacccgagcg ggcatctgga tctgagtcag
       61 cctggggcga gcgcagccgg tgtccaaggg aaccccgatc tgaagaacca agcgaagtgg
      121 cagttcgtac cgtgccccgt caacgggaac gtcatcgttc ggttgaagca gggcaacaat
      181 aacgagttct tcatcgagaa cgagatcttg ccgattcaat cggtgacgtg cgaagggcaa
      241 aacggctctc gcacgagcta tggtgcctgg cacttcgacc agaacgtgaa cggcaagccg
      301 tgcaccgtga ccgacatttc gaaccgaagc atcgacatca cggttggcag cacccaaaac
      361 gagaacgtcg acacgggcgt gcagttcccg aagtgcaact agggggtgtc ccaagtttct
      421 gcccgttgcg agagccaaga aacgcgagcg agaagcacgc ccgaggagtg agcaccggag
      481 gaaggctgaa gcctttcgag gagcggagcg acgaggccgt gcttcgcagc cgcaggatct
      541 tggctcgcag cagggcgggg aacttgggac accccctagc gcgacgcgct gctacccgcc
      601 ccgtactgcc gcatcacgcc cccgaggtcg ggggtcagcg ggctcgggcg tgaccacgtg
      661 gggccggacg agcgagtcgc cctccccgca ggtctgaact tgatcggcga ctctcgagcg
      721 gtgcaaaacc gaggcgatgc agaaaaagac caatcgtcct gagctgcaaa cggtcagcgc
      781 aaggaatcgc gccgcctggc gccgctggct cgagcgacat cacgcaaagg ctcgcggcgt
      841 cgtgctcgtc tatgccaaga agggttcggc tgaagcgtcg atcgattggg ccgagtcggt
      901 ggatgaggcc ctgtgctttg gttgggtcga cggagtgcgc ggcaagctcg acgaaactca
      961 cttcacggtt cggttcacgc cccgtaaatc tggtagcctt tggtcgaagc ggaacctcgc
     1021 gcgcatcgct cacttgaccg aggcgggctt gatgcacccg gcagggcttc gcgccttcga
     1081 gctcggcaaa gcacgcgggg ctcacgagcg cgcgtacgca atccgcgagg aggtcgcgat
     1141 gccgccggag ctcgatgcag agcttcggaa acgcgatcag gcgcgcgctg ctttcgaagc
     1201 gctcgctcgc ggacaaaaaa agggctggta ccgctggatc gccggcgcca agagagaggc
     1261 cacccgcatg caacgagccc gcgacgcgct cctcctcatc gctgcgggcc gcaaagcagg
     1321 cgagactgac aatcaggcgg ctcggcgcgg ggtcccgacc aaagcacgaa tcctgggtcg
     1381 tactcgccgc gactcgtgag cgcgcttacg ctcgaagatc gctctcgagc ccacccgtcg
     1441 catcgagcgt tcgccgagcg aactcttctc gcgactgcga gcgaacgagc ctccctcgct
     1501 cgaacgtgaa cacccaatcg gcgcgcgaag cggccgcggg acggtgcgta gcgaagacga
     1561 tcgtggtgcc gcgttttttc aaccaatcga ggtgctccag cagaatgagc tcggctcgcg
     1621 catcgagtgc cgccgtagcc tcgtcgagca gcaagacgtc cgcttcacga atcagcgcgc
     1681 gagccaccgc aacccgctgc gcttggcccc cggagacatg gctcccctgc tcgcctgcaa
     1741 gcgcgtcgaa gcccctgtcg ccgagctcac tgccatcccc gagcccggcg tcgaacaggg
     1801 cgcgacgcac gtcctcttcg cttgccgtga gtcggccaag acgcgcgttc tcgaggacgg
     1861 agatacagaa gaggtaaggc gtttgtggca ccaccgcgat gcgcgaacgc agcgagtccc
     1921 gcgtcgcgtc acgcaggtcg agctcatccc aacgaaccgc tccgctgtcg ggatcgtaga
     1981 gccgggcgag caacccgagc aacgtgctct tgcccgagcc agacgggccc acgagcgcga
     2041 ccagcgcacc cgctggaatc gacagtgaaa cgtcgacgag agccgggttg tcgccgtggc
     2101 gaacgcacgc ttgcaccagc cgaatctcgc gcgcgggagg cgccaacgtc acgctgccga
     2161 actggagttc ttccttcgcg gtgagcagag cttgaacgcg agcgaaagcg cccgcgccga
     2221 cgccgagttc gacgaactgc tcggctatgg tcacgaaagg ggcgatgagg ccgccgacga
     2281 gcgtgaagaa cgcgaccaca gcgccggtcg acatgctgcc gcgcacgacc agcgccgccc
     2341 ccaacgcgag caccacgaag cggacgccgt gaaacacgaa gttggttccg ccgttcagta
     2401 gcgcgctcag gcgcaccacg gcggtggcgg cggtctgcgt cgacgcgagc aaccgcgcga
     2461 agctttcgtg agcccagcgc tcgagcccaa agagcttcac gatgcgttgg ttttgaatct
     2521 gctcggaggc gagcgcggtg aggcgccctt cgcgatcttg cagctctcgg ctcgcctcac
     2581 cgagacgacg attggtcgcg aagtacagag cgacgaacac gggcgctccc agacccagca
     2641 cgaccgccgc caagagcacg ttgagacgca gcgcgaccac cgtgatcacg acgaggctcg
     2701 cgccctcgaa caagagcgcc gggagcacgc gttcgacgag cccctgtaag cgatccacgt
     2761 cgccggtgag gcgcgccaag aggtcgccgc tcgctgtctc gttgtgatac cggaaggaga
     2821 gggcgtgcac cttctcgatg cagcgagccc tgagctctgc cagaatgcgc actgccaagg
     2881 ctccgctcac gaccgaccag cgatacgcgg cgagcgaacc gagcgaaaac gacaagaata
     2941 cgagcgccga ccacgtgccc aagcgcgcga cgtcacgcga ggggatgact tcgtcgatca
     3001 gcaccttcgt gctccaggga atcacgatgt cggtcgcgag cttcacgagc aggtacgccg
     3061 caaccgcgaa gagctgccgc caatgcgggc ggaagtacgg cgcgagcccg cgcagcaaag
     3121 agcgacgcgc gcccttcatt gctcgcgctc gacgcgcccg tgtgcccgcg cccggccgag
     3181 agcactgcgg tagtgcgccg acaagagctc gtagaatagg tattcgtgcg cgcgctgggt
     3241 cgagcgcatc gtgcggttca cgaacaagtg ggcgaaaatc ggaacgaggt cgagcatcgg
     3301 gcactcgagc tgccccgcgc tctcgaggcc ccgaagttct tcggcaatac gcgcggcctg
     3361 cgctttacgg tgcgcgatcc agtcggcgta acggtattcg gggctttcgg agtcggaagt
     3421 cgccccgcgt gacaacagcg cttcgagccg ggcgcgctcg cctcggaagc gctcgcggaa
     3481 tgcctctttg agcgcgacgt cagcccggaa ttcgagcgca aaggcgtctc ggaggcgcgt
     3541 cgcgagctcg gatcgagccc gcaagtcgag tgaaaagccc tgaaacaggg cgtccattcc
     3601 ggcgatcgtg agctcgaagc gctccgcgtc gccgcctccg tcgagcgccc gctcgacgac
     3661 ctcgagagca gcgtcgctat cgaggtcgaa gtgtcgctcc gccactcgaa cccccgcgag
     3721 cccgccgtag cgctcgacct cccgctcgta cgtgccgatt tcgacccgta cgatgctgcg
     3781 tgaaccaacg aacggcgcga gcgccgcgtg caccgccggc agcacttcgg ccgaaaggcg
     3841 cgaaggctca ccgtgaaagc gcactctcaa gtgaggatcg gggtccgaat accggagaaa
     3901 gaaccaggaa tccgccgcgc cgctcgcccg cacggccctc cgcagcgggg ctatcgcgcg
     3961 ggtgaggaca ccttcgagcg aagcctcgcc gccgtagacc ttcgcgtaaa gccactccga
     4021 cccggggcag aaggtgcgct cgctccgctc gagcgccggg cgcgtgggct ttgaagccgc
     4081 agcttgcgcg ggcgtactcg cactccctcg gcgcacgaaa ggcacgatga gctcgtgctc
     4141 gaagagcccc tcggggcctt tgcaagactc ggccgacgtc aggagttcgt agagcgcgac
     4201 gcgctcacgc gggcgctcca agagagcttc gcaagcgacg acgttctcga ggtcgagcgg
     4261 cagctcgttg tcgtcctcgg cgagcacgac gtgtcgcggg aggctccgcc gcgcccgcag
     4321 agcgcgcagg gccgcgaaac gcgccgcccc cgtgagctcg cgaatcggct gcagttcctc
     4381 ggcagaaacg ttccagcgcg cgcgggacaa aacgagattc tggtaggtca cgcggggcaa
     4441 gaagctagcc tgttcgaagg gcccgaacga aaacgaaagc cccgcactga cgccctggct
     4501 ctgaagcgcg ccgagaaacc tgtacacgct ggggttgcgc ggatcgccat agaagtgcgc
     4561 cgtggatagg cgcggcaaca cttcgcaccc gagctttcga gaccggagga cgacgcgctc
     4621 gccgacgacg cgcacccaca aatcagtgac ggggatctgg gcgggagctg gcgcgcccga
     4681 gcgccccagg tacgggattt cgtagcgacg caacaacggc cgcgccaaca cgttgccgac
     4741 gcgatcttgc gggtggcaaa cgatttccgc gaacacggcg ctcgggcgta gcgcctcctc
     4801 cgcaccgacg tgcgcgcgaa ccaaagcggt gagacgcgga tcccgcgcac agaatcgtcc
     4861 gagcaaacgc gcccccgagg gaccggtcgc gctgtggatc aggagtcgcg gctctgcagc
     4921 agatgtcgag tcgagcacgg tcgccagcac cgcgagtgcg tcgggcaacg gcggcggcga
     4981 gtttgccgcg agcgcttcga tgtcggagtc ggcgagctcg agaacgagct tggagtcgcg
     5041 ccacacccgc tcgagcaacc tcatcagcgc gacgtcgcgg cgaccgaagt cttcgccgtg
     5101 ttccgagctc accggaggga agggcagctc ggcgagcaac gggctatctt cgtgcagcgc
     5161 caacgcttct ccaccgaaac caatgccgct ctcggcatcg agcgcttcga cgagaggcac
     5221 ttcttgctcc tcgtagcgct cgctgaaacg tcgcttgaag tcggcgagcg cgtcgggccg
     5281 cggggcggag atgcgatgca ggaggtgcac cgcgcgctcg agctcgcgac acacgccgct
     5341 gccgagcgtg ctctggccgc aaggcttctc cagatcgact tgaaagagag cgcgcaggtc
     5401 ggctgcggcc ggcgagattg gcacgatgcg ctcggctgtc gccgcgtagc caccctgatc
     5461 gttgccagcg actgcgcgat cgaagcgtgc gaggtcgtcg cccacggcgc gcaagacggt
     5521 cacgaggtcg tcggctccga gttcctcgag cgctcggacc agctgctcga gcgcgtcatc
     5581 gccggtgagc gccggctcga gctccgagac cagaatccgt cgctcgacga gtgccgcaag
     5641 gaaaccgtcc gcatcttcct tcgagacgcc cggccccacc aaggcggccg cgagctctcc
     5701 cagagtggcg ccggtgcgtg cccgttcgag agtcgtggcg agcgcctcgg aactctccac
     5761 tgcgaccagg tgatacgtgc gcctccgacc gtcgcgtcga tcctctacgt aacggaggcg
     5821 ccccgccgcc tcgtaaaggc tcgagttcgc gcggtatcgc aatccacgcc tcgacaagct
     5881 ggactcgagc gcggacgcga tggcgctcac gcactccatg tcgagacgag tccgccttcg
     5941 gtactcgctt cgcgccgcga gctcgagcgc ggtgacctcg ccaacgctgc cggtcgagta
     6001 ccccgcgaag agcccgaaag gcgtcgggcg ggtggccatc cgcgtgaagt accggagcca
     6061 cgattgcgcg gctgcgggct ctcgctcttt ctctgcacac cagcggtcca cgctgcgagt
     6121 gagcgttggc gaggccagga acaccgcctc gcgtagctct ccgcgcttcc agaggcgcgc
     6181 caggcgctcg ctgacgaggg cgtgatcgga ttggagcgcg gccgcaagct cgctcgagtc
     6241 cgcagcatgg gcaggcgcgc tcagccctgc gttcaggctg gtccactcac tgaccggcaa
     6301 gagcggggtg cgcagcacga agcaaccgga cgcctcgtac gagcctcgcg cctgctccat
     6361 ggaggcgacc gcgctcgtcg ccttgggttt ggcggccgag ctcatctcgg aggatccggg
     6421 cgttcactcg cgagaggaga tatcagtagg gctctgtccc agaacggctc gtcgggggtc
     6481 agcgcagaga gcagcgcgag cccgattcca gccgcgccat tcaaaaactc gggttcgtcg
     6541 agccaaacgt cgttcaggtt tgcgtctcgc acgacggctc gaaagcctcc caccccctcg
     6601 ccttcgcgcc gggccgcgag tgtctgctcg aaccaaaaac gactgacgcg cgagaatttc
     6661 ggctctcgag ttgcttcgtg cagccgatga aactggtgcg cggccccggc cgtcccgtga
     6721 cagagccccg cgtcgacgat gcctgccgac tctggagcgc gatcggcaac ggcatgagcg
     6781 agctcgaccg cctcatccca caggtcggtg cgccgaatcg ccgtggcggc tgaaagcagc
     6841 gccagggcga ttccgagatc tccgtagcac caggagagac gcgtcgctgg actcggcccg
     6901 accgcagcct tccagtaggg aaagcgcggg ctcgggctac ctttcgtcgc ctgtcgcgcg
     6961 agccacgcga gcgctccctc gagcagtcgc tcgctgcgtg ccttttcaat gccagccgcg
     7021 cagactcgcg caagaaatgc gatcgtcccg ggcacgccgt gcgcaacgcc gagatcgtag
     7081 cgcccttcgg gatagagtcg tcgatcggcg tcatcgagcg cattcgctgg cgttcgccaa
     7141 gtgacgaggc cgtcgcgctc ctcggcagcc cgctcgagcg cacgaacgat ttcggcgatg
     7201 ccttcctgcg ccagcggacg cgggagccgc tccaacaaat acgctccaat gccgacgctg
     7261 cccgtgatga gatcgagctc ggccgaagcg ttgcgcgcaa cgtactgcgc gagcgcttcg
     7321 tctaccgcgt cgaggtcact gccgacctcc tcgccgcgtt ctcgacacaa gagcgcaaac
     7381 acccaggcaa cgccagaaaa ccccgcaaac agcgaggggt cgagcgactg ctccccgagc
     7441 gcgtcgagag cagcaccgag gtattcgtcg gccagcgccg aggcgccgat cccctcaccg
     7501 cgctcggtgc gagacagcat ctcgaagaag agcgcgatcc cagcgtcacc actcgcgagc
     7561 gaggcttcac gcgctccgct agagcgggaa ggagcgagcg ccgccaaatc tcgagcaatc
     7621 gcaacgatgg cgcgccacgc cgcttgctct tggtcgggct cgagcagatg catgcaatgt
     7681 cgtcgaagct agcgaatttt ttggatccgt tcggctcgtc agcggcatgg gcgtggatcc
     7741 ccccgcaagt ccacgcccga gcccactcag cagcagtgcg ccgtgacttt gctcttggtc
     7801 gaaaccgggc aatcgccctt cgtgtcgcgc ggcttcatgt tggaaccgcc ccgagcgccc
     7861 gac
//
LOCUS       DASTKS010001124         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_848085, whole genome shotgun sequence.
ACCESSION   DASTKS010001124 DASTKS010000000
VERSION     DASTKS010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848085"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2107)
                     /locus_tag="VFQ35_23750"
     CDS             complement(<1..>2107)
                     /locus_tag="VFQ35_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933747.1"
                     /translation="DAKQASAARKALGETSRGAYAELARAIDDSVHGRLKNVASRYMA
                     VVEAVRREDHPDAPLIAWFAAQQASIYRHSDPKLFERYRPFLEAALKAPKNIGWRARA
                     ELADIYQEGLWELGEADVIEKMARQHGCAQNVRLAGPFGRGAALDTLKGYAPERPGPW
                     PVSFEPEPGSGEPPRVLETERDGCYVSAKEASGPGIYYVETYFDVTQPTDVLIAVQGA
                     YAIRVDDHEVLRRDLREWGSWPRFGVAVRLTAGRHRVVARVAEAQSSIRLLHVDGSAL
                     DVPTSADASLPYQLARPQVLPEANVLDRYLVRGTYRDPGQDLLRYLAAMMANVEGQAD
                     VANVLLEPLLAAPDRAAGASLAVSALFTDNDPIFSDSQRQDLSRELRERAVARDPKLW
                     HPRLLLAVGRADREGRVEAAREVKKLVAEFPEVPAVSSELMRLYSELGWKVEFAQSAL
                     DLAARFPDEPDALSAAVEVLEAQGRSAEADKLVERIQKLDPDQEIRLRRALDRLDFPT
                     ALAELKRLAARRPKRKDLPERIYDVLVRAGNPGEVLKKLELALEQEPKNEKARLDLAD
                     VHYALGKHDALVKALVEAVTRGAPSARLEEAIDLVEGISELEPYRHDARKVIAEYERS
                     GQQLEGTAARVLDYAAVWVHSDGSSRMLEHEIIKIQSAEAIADQAEQPLQQGVLLHMR
                     VIKADGRTLEPELVEGKPTL"
BASE COUNT          329 a          748 c          671 g          359 t
ORIGIN      
        1 agcgtgggct tgccctccac gagctccggc tcgagcgtgc gaccgtccgc cttgatcacg
       61 cgcatgtgca gcaggacgcc ctgctgcagc ggttgttccg cttgatcggc aatggcctcg
      121 gcgctctgaa tcttgatgat ctcgtgttcg agcatgcggc tcgagccatc cgagtgtacc
      181 caaaccgcgg cgtagtcgag cacgcgcgcg gcggtgccct cgagctgctg cccggaacgc
      241 tcgtactcgg cgatcacctt gcgcgcgtcg tgccgatagg gctcgagctc actgatgccc
      301 tcgacgagat cgatcgcctc ttcgagccgc gcggacggag cccctcgagt aaccgcctcg
      361 accaaagcct tcacgagcgc gtcgtgcttg ccaagcgcgt agtggacgtc cgccaaatcc
      421 aggcgggctt tctcgttctt cggctcttgc tctagcgcca gctccagctt cttgagcacc
      481 tcacctgggt tacccgcgcg aaccagcacg tcgtaaatgc gctcgggcaa gtccttgcgc
      541 ttggggcgac gcgccgcgag gcgcttgagc tccgcgagcg cggtggggaa atccaagcgg
      601 tcgagcgcgc gccgcaggcg gatctcttgg tcgggatcga gcttctgaat gcgctcgacc
      661 agcttgtcgg cctccgccga gcggccttgg gcctccaaca cctccaccgc cgccgagagc
      721 gcgtccggct cgtcggggaa acgtgcggcc aagtccaacg ccgattgagc gaactctacc
      781 ttccacccga gctccgagta cagccgcatc agctcactcg agaccgccgg cacctccgga
      841 aactctgcga ctagcttctt tacctcgcgc gccgcttcga ccctgccctc tcggtccgca
      901 cggcccacgg cgagtagcag cctcgggtgc cacagcttcg gatcacgcgc gaccgctcgc
      961 tcgcgcagct cgcggctgag gtcctggcgt tgcgaatcgg agaagatcgg gtcgttgtcc
     1021 gtaaacagcg ccgagaccgc gagtgacgcc ccggccgcgc gatccggcgc ggccaacaga
     1081 ggctccagca agacgttggc gacgtcggct tgcccttcga cgttggccat catcgccgcc
     1141 aaataacgca gcaagtcttg gcccgggtcg cggtaggtgc cacgaaccag ataacgatcc
     1201 aacacgttcg cttcgggtaa cacctgcggt cgcgcgagtt gatagggcaa cgacgcatcc
     1261 gcgctggtgg gcacgtcgag cgcgctgccg tccacgtgga gcagccgaat cgagctttgg
     1321 gcttccgcca cgcgcgcgac gacgcggtgg cgccccgccg tgagccgcac cgccacgccg
     1381 aagcgcggcc aggatcccca ctcccgcaga tcccgccgca gcacctcgtg atcatcgacg
     1441 cggatggcgt aggcgccctg caccgcgatc agcacgtcgg tgggttgggt cacatcgaag
     1501 taggtctcga cgtagtaaat gcccggaccg gacgcctcct ttgccgacac gtagcagccg
     1561 tcgcgttccg tctcgagcac gcgcggtggc tcacccgacc cgggctccgg ctcgaagctg
     1621 accggccatg gcccaggccg ctccggcgca taacctttga gcgtatcgag ggcggcgccg
     1681 cgaccgaagg ggccggccag gcgcacgttc tgagcacagc cgtgctggcg cgccatcttc
     1741 tcgattacgt cggcttcgcc gagctcccac agcccttctt gatagatgtc ggcgagctcc
     1801 gcgcgcgcgc gccagccgat gttcttcgga gctttcaggg ccgcctccaa gaacggtcga
     1861 taacgctcga agagcttggg gtcgctgtgc cggtaaatcg acgcctgttg cgccgcgaac
     1921 caggcgatca acggcgcgtc cgggtggtcc tcgcgacgca cggcttcgac cacggccatg
     1981 tagcgactcg cgacgttctt gagtcgcccg tgaacggagt cgtcgatggc gcgcgcgagc
     2041 tccgcgtaag cgccgcgcga cgtttcgccg agcgccttgc gcgcggcgct cgcttgcttc
     2101 gcgtctc
//
LOCUS       DASTKS010001125         5239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_179419, whole genome shotgun sequence.
ACCESSION   DASTKS010001125 DASTKS010000000
VERSION     DASTKS010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179419"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1594)
                     /locus_tag="VFQ35_23755"
     CDS             complement(404..1594)
                     /locus_tag="VFQ35_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933748.1"
                     /translation="MTTKTPSRATHRERRLGPLALAFALACATSGCSSETALFTASSL
                     GGGTSFGGALASGGFSSIGGAVSEGGTGAGGTATSPAPDNRGGSDSLANGGHNDGGRT
                     TAGAKNEGGAASSDSGGSSASQGGASAGVNSGGSSASAGAAGKGSCCSDGDCLCHGPA
                     PTALTSKNGPYGTASLRLQTGTLHYPTDAEPPLAGIAICPGFLNTGPEMGPWGPFYAS
                     WGIAVIVTDTTGADLPNVRGDKLVAAIEELKAQNMASGPLAGKLAGRYGTSGYSMGGG
                     GTTFASAKDPTYRTSIGLAAWGPDGTNVKVPTLFLCSDTDTVAGCGYSQTAYDAMGST
                     SKMLIKVPNATHFNWFDPTDAGQGMSGEYALAFQKVFLEGDQRWRPLLLAKPAQGSGA
                     TNIQ"
     gene            complement(1575..2327)
                     /locus_tag="VFQ35_23760"
     CDS             complement(1575..2327)
                     /locus_tag="VFQ35_23760"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HET9933749.1"
                     /translation="MSEEYSRDSEVHVSRASCPPAPHHVALFLVPDERSPPTAHRERA
                     PSAALLCDAVLELQALGVEHLTLQAFGDLSAWVCRAAPSEARRFSDFVAYATPCLVAA
                     GIRVAALGALEELAPSLRRGLERLIHVTRRGRGMSLTLLISYRGRTDIVETARHLATH
                     VRAGLLIPEDIDEELFRTRMQAAEVPNFDVCIRLHDAPPPRDLFPFQSSNAETLDVAC
                     DIADIGAELARALQSTQSHRSLTLEHSDYQDT"
     gene            2450..3712
                     /locus_tag="VFQ35_23765"
     CDS             2450..3712
                     /locus_tag="VFQ35_23765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9933750.1"
                     /translation="MSANWFLKTRSELLDAILAPPKSTEEAQGGGSKPPRAPSSRQRV
                     WFAVGVFVLALGLYAARTLGARSEVEVTVHPVSRGRIRERVPAVSLGRVSARRELALR
                     AEFPGRVQRIEHRTGERVTAGEELLQLSAQELSDSVVRAEKALATARAQAAEAATRAE
                     LAVRRGRRAATLLASGSIPPAEGEAARLESRLAQQTALTALARVGEATAARDAARTAL
                     SNSVVRAPWDGIVLELHVEEGAAVTGGSMLLTLADDSALHVAASIDEADLGRVSVGMP
                     VELAFEATGESPQQRRVSSIDPSVIRGERGARVVALRVDLDVGTHLPVGSGVQVEVTS
                     KENASALLVPVNAVTGTGSLRDVYVAVDGVARRRVVEVGIASWDSLEVKRGLSLGELT
                     ILNPGQAKLEDGGRVAPRLDDAASGGRR"
     gene            3709..4410
                     /locus_tag="VFQ35_23770"
     CDS             3709..4410
                     /locus_tag="VFQ35_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933751.1"
                     /translation="MSAAPLLRIRHLGRRFQVGGEEVEALADVSFDVHPGEYVSIVGP
                     SGSGKSTLLQMLGCLDTPSSGSYRLAGEEVVSLSDSEKARVRNLHIGFVFQGFHLLPR
                     TSVLDNVCLPLTYRNHSAAERRRLALEALARTRMLPRCQHWPNQLSGGERQRVAIARA
                     IVTRPSLLLCDEPTGNLDQRVSLEITRTFEELNTSVGVTLIVVTHEPELARRARRTLR
                     LVDGRLVYDGPGGLT"
     gene            4407..>5239
                     /locus_tag="VFQ35_23775"
     CDS             4407..>5239
                     /locus_tag="VFQ35_23775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933752.1"
                     /translation="MSNQLQHVKSAALSLAANKTRSALTMLGISIGVLAVTLLVSVGE
                     GAKSSVETSLTSIGTNLLSVVPGRIEAQGFGYLGAAVRKPLVIEDSELLERQATHLAA
                     VTPVVLGNASIQFAGRARSTNIFGVGPRFLDVRNIRLESGSFLSSEDIAARRRVVILG
                     RTLVDSILDGKPAIGSSVRVGGERFRVVGVTERKGRSLGIDLDDMALVPVSVAAEFFE
                     QTSLNQILAAAKSDTDAELASQQIDELLAGRRRGERSFTIQTQDDLLRVFDSITSAMS
                     WT"
BASE COUNT          852 a         1663 c         1772 g          952 t
ORIGIN      
        1 cgcgggccct aagccgacaa actctcaggg gtttgccgga gcatcgggag agcgggatcg
       61 gcacggcggc gccaccgttc gggaaagtct cttcacaatt caggccgcga tttgccgcgg
      121 cgcatatcgc ctccggaagc caagcgtctt cattcggaag cgtatcggca cgagcgcgcg
      181 cgtttcgtta cgttttcgcg acttcgtgcg tttccgcgga ggacaccccc tagtgggttc
      241 gcgttgtttc atctcgatgt tgtcgcgctg tctcatcgtg atatcgcatt gtccgcagcc
      301 gggccccgac gctcgaaaag gggggacaga gttgggcctt gctttttgcg tcgcattggc
      361 tcgcccgtgt cgctcgacgc atgagcaccc atgcgacatg cgctcactgg atattcgttg
      421 cgccgcttcc ttgggcgggt ttcgcgagca gcagtgggcg ccagcgctgg tcgccctcga
      481 ggaagacttt ttggaaagcg agcgcatact cgcccgacat gccctgaccc gcatcggtgg
      541 ggtcgaacca gttgaagtgg gtcgcgttcg ggaccttgat cagcatcttg ctggtcgagc
      601 ccatggcgtc gtaagcggtc tgcgagtagc cgcagccggc gacggtgtcc gtatcgctgc
      661 aaaggaagag cgtgggtacc ttcacgttgg tgccgtcggg tccccaagcc gcgagcccga
      721 tcgacgttct gtaggttgga tcttttgccg acgcgaaggt ggtgccgcca ccacccatcg
      781 agtagccact cgtgccgtat cgcccggcca actttcccgc gagcgggccc gacgccatgt
      841 tctgggcctt gagctcttca atcgccgcga ccagcttgtc tcctcgcacg ttgggtagat
      901 cggccccggt cgtgtcggtg acgatcaccg cgatgcccca agaagcataa aatgggcccc
      961 acggacccat ttcaggccca gtattcaaga agccggggca gatggcgatg cccgcgaggg
     1021 gcggctcggc gtcggttgga tagtgcagag tgcctgtctg gagacgaagc gaggccgtcc
     1081 cgtaaggccc attcttcgaa gtgagagcgg tcggcgcggg gccatggcag agacagtcgc
     1141 cgtcgctgca gcagctgcct tttccagcgg ctcctgcgct cgcgctgctc ccgcccgagt
     1201 tcacgcccgc tgacgcgccg ccttgagagg cgctgctacc gccactgtcg ctgctcgctg
     1261 cgccgccttc gttcttcgct cccgctgtgg ttcttccgcc gtcgttgtga cccccattgg
     1321 ctagagaatc gctccctccc cgattgtctg gagcgggact ggtcgctgta ccacctgccc
     1381 ccgtgccgcc ttcgctcact gcgcccccga tactcgagaa gcccccgctc gccagcgctc
     1441 cgccaaagct cgtgccgccg ccgagtgagg acgccgtgaa cagtgcggtc tcgctcgagc
     1501 agccgctggt tgcgcacgcc agcgcgaagg cgagcgccaa tggtcccaag cggcgctctc
     1561 ggtgcgttgc gcgactaggt gtcttggtag tcactgtgct cgagtgtcag cgacctgtga
     1621 ctttgggtgg attgcaacgc gcgtgcgagc tccgcaccga tatcggcaat gtcgcacgcg
     1681 acatccagtg tttcggcatt gctggactga aatgggaaca ggtcccgagg gggtggcgca
     1741 tcgtgcaggc gaatgcagac atcgaagttc ggcacttccg ccgcctgcat tcgcgtgcga
     1801 aatagttctt cgtcgatgtc ttccgggatc aagagcccgg ctcgaacgtg cgtcgcaagg
     1861 tgtcgcgcgg tttcgacgat gtcggtgcgt cctcggtaag aaatgagcag ggtgaggctc
     1921 ataccgcggc cgcggcgagt cacatggatc agacgctcca agcctcgacg caatgacggt
     1981 gcgagctctt cgagagcgcc gagggcggcg acgcgaatcc cggcggcgac gaggcatggg
     2041 gtagcgtaag cgacaaaatc actgaaacga cgggcttcgc tcggggccgc gcggcacacc
     2101 caggcggaga gatcgccgaa agcctggagc gtgaggtgtt ctactcccag ggcttgtagc
     2161 tcgagcaccg cgtcgcagag cagagccgcg ctcggcgctc tctctcggtg cgcggttggc
     2221 ggcgaccgtt cgtcgggcac gaggaacagc gcgacatggt gcggcgcggg ggggcacgac
     2281 gcgcggctga cgtggacttc gctgtctcta gagtactctt cgctcacttc cgacatgtaa
     2341 ggtaggagta cagccagcga gccgattcgg gtggtcgggc caaaccctac ccgctgaaaa
     2401 aagatccgcc cgctttcgcg tcagccatcc acaggcagtc tggcgacgaa tgagcgcaaa
     2461 ctggtttctc aagactcgaa gcgagctcct cgatgcgata ctcgcgccgc ccaagtccac
     2521 ggaggaggcc cagggagggg gctccaagcc cccccgcgct ccgagctctc gtcaacgcgt
     2581 gtggttcgcg gttggcgttt tcgttctcgc tctcgggctc tacgcagcgc gaaccctcgg
     2641 tgcgcgctcc gaagtcgagg tgacggttca ccccgtttcg cgcggcagga tccgcgagcg
     2701 cgtaccggcc gtatcgctcg ggcgcgtcag tgcgcggcgc gagctagctc tccgtgcgga
     2761 gttcccgggg cgcgtacaac ggatcgagca tcgcacggga gagcgggtca cagctggcga
     2821 agagctgttg cagctctcgg cccaagagct ctccgattca gtcgtcagag ccgaaaaggc
     2881 gctcgcgacg gcgcgggcgc aggcggcgga ggcggcgacg cgcgccgaac tcgcggtgcg
     2941 acgcggccgg cgcgccgcaa cactgctcgc ttctggcagc atcccgcccg cggaggggga
     3001 ggcagcgcgc ctcgagagca ggctggctca acagaccgca ttgaccgccc tcgcgcgcgt
     3061 cggtgaggcg acggcagccc gcgacgcggc gcgaaccgcc ctttcgaaca gcgtggtacg
     3121 cgcgccctgg gacggcatcg tgctcgagct tcacgtcgaa gaaggcgccg ctgtaacggg
     3181 aggctctatg ctcttgacgc tcgccgacga cagcgcgctt cacgtcgcgg cgtccatcga
     3241 cgaggccgat ctcgggcgcg tgagcgtcgg aatgccggtc gagctggcct tcgaagcgac
     3301 gggcgagagt ccgcaacaac gccgcgtttc gtcgattgac cccagcgtga ttcgcggcga
     3361 gcgcggcgcg cgggtggtgg cgcttcgagt cgacctcgac gtcggcacgc atcttccagt
     3421 cggaagcggc gtccaggtgg aggtgacttc gaaggagaat gcgagtgcat tgctggtgcc
     3481 ggtcaacgcc gtaacgggga cggggagcct gcgagatgtg tacgtcgccg tcgatggcgt
     3541 ggcgcgccgc cgcgtagtcg aggttggcat cgcgagctgg gacagcttgg aggtcaagcg
     3601 cgggctgtcg ctgggagagc tcacgatctt gaatccaggg caggccaaat tggaggatgg
     3661 gggccgcgtc gccccgcgtc tggacgatgc ggcttcgggt ggccggcgat gagcgccgcg
     3721 ccgctgctcc ggattcgcca cctggggcgt cgctttcagg tcggcggcga ggaggtagag
     3781 gcgctcgcgg atgtgtcttt cgatgttcat cccggcgagt acgtctcgat cgtgggtccg
     3841 tcggggtccg gcaagagcac gctgttgcaa atgctcggtt gcttggatac gccgagttcg
     3901 gggagttatc gcttggccgg cgaggaggtc gtgtcgctca gtgactcgga aaaggctcgc
     3961 gtgcgcaatc tccacattgg cttcgtcttt cagggatttc atctgctgcc gcggaccagc
     4021 gtgctcgaca acgtgtgctt gccgctcacc taccgcaacc attccgcggc ggagcggcgc
     4081 cggcttgctc tcgaagcgct cgcacgaacg cgcatgctcc cacgctgcca acactggccc
     4141 aatcagctgt cgggcggcga gcggcaacgc gtcgcgatcg cgcgcgccat cgtcacccgg
     4201 ccatcgctct tgttgtgcga cgagcccacg ggcaacctcg atcagcgggt tagcttggaa
     4261 attactcgaa ccttcgagga gctcaacacg agcgtcgggg tcaccttgat cgtggtgacg
     4321 cacgagccgg agctcgcgcg ccgagcgcgt cgcacgctgc gcctcgtcga tggtcgcttg
     4381 gtctacgatg gcccaggggg gctcacgtga gcaatcagct tcagcacgtc aagtcggccg
     4441 cactatcgct cgcagccaac aaaacgcgga gcgcgctcac gatgctcgga atcagcatcg
     4501 gcgtcttggc ggtgactttg ctggtcagcg tcggtgaagg ggcgaagtct tcggtcgaga
     4561 cgtctctgac cagcatcggc accaacttgc tcagcgtcgt gcctgggcgc atcgaggccc
     4621 aaggcttcgg ttacctgggc gccgcggtgc gcaagccgct cgtaatcgag gactccgagt
     4681 tactggagcg ccaagcgacc cacctcgcgg cggtgacgcc cgtggtgttg ggcaacgcga
     4741 gcattcaatt cgccggccgc gcgcgtagca ccaacatttt cggcgtcggc ccgcgcttcc
     4801 tcgacgtgcg caatatccgg ctggagtcgg gcagcttctt gagctcggag gacatcgccg
     4861 ctcgcaggcg agtcgtgatc ttggggagaa cgttggtcga cagtatcctc gatggcaaac
     4921 cagcgatcgg tagcagcgtg cgcgtcggcg gcgagcgctt ccgcgtggtc ggcgtgacag
     4981 agcgcaaggg gcgctcgctc ggcatcgatc tcgatgacat ggccttggtt cccgtctccg
     5041 tagcggccga gttcttcgag caaacgagct tgaaccagat tctcgccgcc gccaagagcg
     5101 acaccgacgc cgagctcgcg agccagcaga tcgacgagct gctcgcaggc cgccgccgcg
     5161 gcgaacgctc gttcacgatt caaactcagg acgacctctt gcgagtgttc gactcgatca
     5221 cgtccgcgat gagctggac
//
LOCUS       DASTKS010001126         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_179441, whole genome shotgun sequence.
ACCESSION   DASTKS010001126 DASTKS010000000
VERSION     DASTKS010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179441"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..932
                     /locus_tag="VFQ35_23780"
     CDS             183..932
                     /locus_tag="VFQ35_23780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HET9933753.1"
                     /translation="MALPAHLAERFSPALSRALRLGSVSPSAANTADAVAFGIPSLDA
                     VLPDGGFLRGGVVELAVGEGVPATSLALSACEALQRSHQSQGRALPWCAFVDPSNTLY
                     APGVVESGVRPERLLVVRPPVEALARTLLKLAKSPAFELILVDTIGVPGASVRLALDS
                     WPRIIRRLSLEVQSSERTIVLLTDRTLPRALPLPVAQRIDVTRPARDRLMVQVSKDRR
                     GHITPLRPVAWSRPRIGQSSEREEVRHASAG"
     gene            916..>2449
                     /locus_tag="VFQ35_23785"
     CDS             916..>2449
                     /locus_tag="VFQ35_23785"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET9933754.1"
                     /translation="MLQPGRKHDFTPQPLAELPLLARVQLSAPPTPVAAEASARRVVA
                     LVLPALLCELAETPAFAALGEEVKATPRSPRRRQLPLGVVLSDTPSSEASSASVEEIP
                     ATAVLSAINEAASRYGVRVGQSLAEAKALVSRLVVRRVPRAAIEQGLSRVAEVALAFG
                     ATVALAAPDTVWVDVTGAAHLFGGEAALLAELVGRVRETGHRVRAALASGPLLAQAFA
                     RFGELGGEGYLIVTPSHEQREFARLPVRALALESSVESWLIRLGVLTISGLKELPQAA
                     LAARLDEHAGRVLDLMAGRDSTPLVPYVPAPTLSEESSWDEGVDGSTPLLFVLRGLAT
                     RLGSRLAGRGQAARNLVLEIAAERSIARFRGAPTSTRIEFSLPKPLWKSSEIYRIVAS
                     KLERLSLPAPSLGLRLEVPELVEAMPRQLELGFWGGSASQALDELPIVLAELAADIGE
                     DRIGVLKVADSHRPELASSLVPALPNTPHTKSRVKKARKPQPVQVTAPEGAGKLVHRL
                     TRL"
BASE COUNT          357 a          770 c          825 g          497 t
ORIGIN      
        1 tgcgggcccg ttggtgcgaa gtttgctgct cttcggagga tgcgttcgga cgctgagctc
       61 ggacattgtt tgtctcgggc ggacttgcat tcgcgataac tgatagtatg ttcagtatca
      121 cgaacggcgc tgctctttcc tcgaacctaa gcagcccatc cccactttca aggagtcgag
      181 cgttggcctt accagctcat ctggcggagc gattctctcc ggcgctttct cgggcgctgc
      241 ggcttggcag tgtgagccct tcggcagcca atacggctga cgccgttgcg tttggcattc
      301 cgtctctcga tgcggtgctg cctgacggcg ggttcctgcg tggcggcgtg gtcgagcttg
      361 ccgtcggcga aggcgtgccc gcgacctcgc tagcgctgtc tgcgtgtgag gcgttgcagc
      421 gcagccatca aagccagggc cgcgcgttgc cgtggtgcgc tttcgtagat ccgagcaaca
      481 cgctgtacgc gccgggcgtc gtcgagagcg gggtccggcc ggagcgtttg ctcgtcgtgc
      541 gccccccggt cgaagcgttg gcgcggacgc tgttgaaact cgccaagtcg ccggcgttcg
      601 agctcatctt ggtcgatacc atcggcgtac cgggagccag tgttcgcctc gcgctcgata
      661 gctggccgcg catcattcgt cggctctcgc tggaggtgca gagcagcgag cgtaccatcg
      721 tcttgctcac ggaccgcact ttgccacggg cgctgccgct gccagtggcg cagcgcatcg
      781 acgtgacgcg gccagcgcgc gatcgcctga tggttcaagt cagcaaagat cgtcgcggcc
      841 acatcacccc ccttcgtccc gttgcctggt cgcggcctcg aatcgggcag agctccgagc
      901 gcgaagaggt tcgccatgct tcagccgggt agaaagcacg attttactcc gcagccgttg
      961 gctgagttgc cgctcttggc gcgcgttcag ctttcggcgc cgccaactcc cgtagcggct
     1021 gaagcatcgg ctcggcgtgt cgtcgcgctc gttctgcctg cgctcctgtg cgagctcgct
     1081 gaaacgccag catttgcggc tctaggcgaa gaagtgaagg caacgccgcg ctctcctcgc
     1141 cggcgccagc tgcctctcgg tgtcgtgctc agtgacacgc cgtcgtccga ggctagctcc
     1201 gcatccgttg aagaaattcc ggcaacggcc gtgctcagcg cgatcaacga agccgctagc
     1261 cgttacgggg tgcgcgtcgg gcaatcgctc gccgaggcaa aagcactcgt ctcgcgtttg
     1321 gtggtgcgtc gtgtgccgcg tgccgccatc gaacaagggt tatcgcgtgt ggccgaggtt
     1381 gcgcttgctt tcggcgcgac ggtggcgctc gctgcgcccg atacggtctg ggtagacgtg
     1441 accggggccg cgcatttgtt cgggggagag gctgcgcttt tggcagagct cgttggtcgc
     1501 gtgcgcgaaa cgggtcatcg cgtgcgggct gcgctcgctt cagggccgct cttggcgcag
     1561 gcgttcgcgc gctttggcga actcggaggc gaggggtatc tcatcgtgac gccatcgcac
     1621 gagcagcgtg agtttgcgcg cttgccggtg cgcgctctcg ctttggagag ctcggtcgaa
     1681 agctggctga tacgtctggg tgtgctcacc atttccggct tgaaagagct gcctcaagca
     1741 gcgctcgcgg cgcggctcga cgagcacgcc gggcgcgttc tcgatctaat ggcggggcgc
     1801 gactcgacgc cgctcgtccc gtacgtgcca gcacccacgt tgagcgaaga gtcgagctgg
     1861 gacgaaggtg tagacggcag cacgccgctt ttgttcgtgc tgcgcgggct cgcgacgcga
     1921 ctgggctcgc ggctggctgg gcgtgggcaa gcagctcgca atctagtgct cgaaatagcc
     1981 gctgaacgca gcatcgcacg ctttcggggc gctcctacgt cgacgcgcat cgagttctct
     2041 ctgccgaagc cgctttggaa gagcagcgaa atttatcgca tcgtcgcttc caaactcgag
     2101 cgactttcgt tgcccgcacc gtctctcggc ttgcggctcg aagtgccaga gctcgtcgaa
     2161 gccatgccgc gccagctcga gctcggcttt tggggaggga gcgcctccca agcgctcgac
     2221 gagcttccga ttgtgctcgc cgagctcgcc gccgacatcg gcgaggatcg gatcggggtg
     2281 ctgaaagtag cggattcgca tcgcccggag ctcgcctcga gcttggtgcc ggcgttgccg
     2341 aacacgccgc acacgaaatc acgtgtgaag aaggcgcgta agcctcagcc ggtgcaggtc
     2401 acggcgccgg agggtgcggg taagctcgtt catcgcctca ctcggctct
//
LOCUS       DASTKS010001127         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1297020, whole genome shotgun sequence.
ACCESSION   DASTKS010001127 DASTKS010000000
VERSION     DASTKS010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297020"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VFQ35_23790"
     CDS             complement(<1..1660)
                     /locus_tag="VFQ35_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933755.1"
                     /translation="MSRTSNAVWLGALVASTVACEDGTFVGSRGPGGSGGANSLGGNV
                     SVTTGGTGARTSGGAAATFGGEFNGGAGFERGGSASGGTFLGAGGSLLGLGGNDEPRG
                     GQTNAEGGSSGGVGGSSGGVGGSDFARGGATTGGADNSRGGAASGGSGDAHGGAGGAT
                     ATCSDGVRNGDELGIDCGGACKLCGGASCETDTVCASGVCDRIAGTPRKCREVSCVNH
                     RVDPGEVGVDCGGGGCALCPGAACEFDDHCESRNCGGFCLASACDDGVKNGLETGVDC
                     GGSCKLCAAAPCTDPAQCQSGVCTGGTCRAPTCDDGVLNGRETDLDCGGSCAACGGSF
                     CYAPADCASRICNRGQCTESSCNDGILNGRETNVDCGGSCLRCGGIACQSDGECSSRH
                     CVDGRCSAPTCTDGFRNNRESDIDCGGPCAPCSTELICWSNSDCASGVCKNDRCVASS
                     CSDGVRNGNEREIDCGGACAECSTSITCASDQDCPSGRCRDAVCTPLPSCLGTPALSC
                     QGRSCCEAPVVPAGEFWLGGDPVNPAAQACTWTTSGISRAKLHSF"
     gene            complement(1699..2844)
                     /locus_tag="VFQ35_23795"
     CDS             complement(1699..2844)
                     /locus_tag="VFQ35_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933756.1"
                     /translation="MKHGLSVCKLGVWAAIGLAWAASSATAAAEMAAAANAEHELATL
                     EVSAVFVGMAGSEQLAAKTAAWFVGHALRFSSREDDAVDTEAWLRSSANGLRILVRET
                     ATGRLRLWFVLQRDGARRYLVREQTLPSGLDEVGLENIAQLLFSSALALAEGREETPV
                     ERVPEAPRFASEGVESVRERAPRRASVARQSGSIPSDPARASREGALQLGGAVGYELR
                     VSGPEGIFQGPVLALGVQQKRSDWDRTLTLAATLLVPGGFESTDYAVNTKGYSGRVQA
                     LAKRSGGDALSPEILLALGLDWLTLSPETLSTARFTASGERRSLRPFVAIGGGLSFRI
                     GALSLSTELEAVAHLSRTHYDVEEGGVRRESLVAWPLQPALRVALRW"
     gene            complement(2845..3987)
                     /locus_tag="VFQ35_23800"
     CDS             complement(2845..3987)
                     /locus_tag="VFQ35_23800"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9933757.1"
                     /translation="MRSREPFDLLARARRQPLTAVEEEELSALLEEDAGARCFLEAGL
                     EADRQTAAHPDDEALITRMARRAELAALQGSSASSTQTRPQRANRVGRELRATPALAA
                     AKARPQHSNRVGRALRATPALAAAICFACGAFAGVGAIAVYSAVTPAPAASARSTTPE
                     RDRERAKAPQAAPSRNNVPASPEDDAPALLAAPVPADTVSPALSGSKVRALPSAEGFA
                     PVRPLAQPSLVAPKALAPAVSVSDQSTPAELPPAPSTANVSAAELYQRANRARQEGRT
                     KDALETYRELQASYPVSREAVASHLTMGNLYLLQGNAALALEQFRAHRTAGGGGFEPE
                     SLWGEANALRKLGRVDEERAVLSELVSRYPNSAYELPAQRRLRTSD"
     gene            complement(3984..4556)
                     /locus_tag="VFQ35_23805"
     CDS             complement(3984..4556)
                     /locus_tag="VFQ35_23805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933758.1"
                     /translation="MAAAARKPEAVRGFLLAVGPHVVRVVRGVLGPRDPDVEDVAQEC
                     SFALIDALATFRGECAVVHFVRRITLLTALNARRRERAQKRARERDLGAEVDSFAASD
                     PGPERQVAARASLGVIRELLATLPAGQAEALALHVVLGYTLAEMQDASGIPLETWKSR
                     LRLAKGALRTRMLADPRGSELWLERGEESP"
BASE COUNT          865 a         1802 c         1554 g          765 t
ORIGIN      
        1 ggaaggaatg gagcttggcg cgcgaaatcc cggaggtggt ccaagtgcag gcctgcgcgg
       61 cagggttgac cggatcgcca cccagccaga attcgcccgc agggacgacc ggagcttcgc
      121 aacagctccg gccctggcag ctgagagccg gagtgccgag acacgagggc agaggggtgc
      181 aaaccgcgtc gcgacagcgc cccgagggac agtcctgatc gctcgcgcac gtgatcgagg
      241 tgctgcactc cgcgcacgct ccgccgcagt cgatctcgcg ttcgttgccg ttccgcacgc
      301 cgtcgctgca gctgctcgcg acgcagcgat cgtttttgca cacaccgctc gcgcagtcgg
      361 agttcgacca gcagattagc tcggtcgagc aaggggcaca ggggccgccg cagtcgatgt
      421 ccgactcgcg gttgttgcgg aaaccatcgg tgcaggtcgg agcgctgcaa cgaccgtcga
      481 cgcaatgccg actgctgcat tcgccgtcgc tctgacaagc gatgccgccg caacgcaagc
      541 aggagccgcc gcagtcgacg ttggtttcac gaccgttgag gatgccgtcg ttacagctac
      601 tctcggtgca ctggcctcgg ttgcagatcc tgctcgcgca gtcggcggga gcgtagcaaa
      661 agctaccccc gcacgccgca cagctgcccc cgcagtcgag gtccgtttcg cggccgttca
      721 gtacgccgtc gtcacacgtt ggcgcccgac aagtcccgcc ggtgcacacg ccgctctgac
      781 attgggcagg atcggtgcag ggggccgccg cgcacagctt gcacgagccg ccacaatcga
      841 cgcccgtttc caaaccgttc ttcacgccgt cgtcgcacgc cgaagcaaga caaaatccgc
      901 cgcagttgcg gctctcgcaa tggtcgtcga attcgcaggc cgcgccaggg cacagcgcac
      961 aaccgccgcc cccacaatcg acgccgacct cgcccgggtc gacgcgatgg ttcacgcaac
     1021 tgacttctcg gcatttgcgc ggagtaccag cgattcgatc gcaaacgccg ctcgcgcaca
     1081 cagtgtcggt ttcgcagctt gcgccgccgc acagcttgca cgcccctccg cagtcgattc
     1141 cgagctcgtc accgttgcgc acgccgtcgc tgcacgttgc cgtcgcacca cccgcaccgc
     1201 cgtgcgcgtc accactgcca ccactggcag cgcccccgcg tgagttgtcg gcgccgcccg
     1261 tggtggcacc gccgcgcgca aaatcgctcc cgcccacgcc gccggagctt ccacccacgc
     1321 cgccggagct tccgccctcc gcgttagtct gcccgccacg cggctcatca ttgccaccga
     1381 gtcccagcaa gctaccccca gcccccaaga aggtgccgcc gctcgcgctg cccccgcgtt
     1441 cgaagcccgc gccgccgttg aactcgcctc cgaacgtcgc ggccgcaccg cccgaggttc
     1501 gcgccccggt tccacccgtc gtgacactga cgttgccgcc aagggagttg gctccgccgc
     1561 tgccgcctgg accgcgcgag ccaacgaagg ttccgtcctc acacgcaacg gtgctcgcaa
     1621 ccaacgcgcc aagccagacc gcgttcgatg ttcgactcat gccggctaag taacgagcac
     1681 tccaaaagcg agtcatgact accagcgcaa cgccactcgc aatgcgggtt gaagcggcca
     1741 agccacgagc gactcacgtc gaacgccgcc ttcttcgacg tcgtagtgag tgcgcgagag
     1801 atgcgccacc gcctcgagct cggtcgatag cgagagggcg ccgattcgaa acgacagccc
     1861 gccgccaatc gcaacgaagg gccgcaacga gcgtcgctcg ccgctcgccg tgaagcgcgc
     1921 agtcgaaagg gtctccgggc tcagcgtgag ccaatcgagc ccgagcgcga gcaaaatttc
     1981 ggggctcaac gcatcgcctc cagagcgctt ggccaaagct tgcacccgcc ccgaataccc
     2041 cttcgtattc accgcgtagt cggtggattc gaaaccgccc ggaacgagca acgtggccgc
     2101 cagcgtaagg gttcggtccc aatcggaccg cttctgctgc acgcccaacg cgagcacggg
     2161 tccttggaag atgccctccg gccccgaaac gcgcagctcg tatccgacgg ctccgccgag
     2221 ctgcagagcg ccctcgcgcg acgcgcgggc cgggtcgctt ggaatcgagc cggactgacg
     2281 agcgacggaa gctcggcgag gtgcccgctc acgcacggac tccactcctt cggacgcgaa
     2341 gcgcggagcc tctgggactc gctcgacggg cgtctcttcg cgcccctcgg ccagcgcgag
     2401 cgccgacgag aagagcagct gagcgatgtt ctcgagaccg acctcgtcga gccctgacgg
     2461 cagggtctgc tctcgcacca agtaccttcg cgcgccgtcg cgctgcagca cgaaccatag
     2521 ccgcagccgg cccgtcgcag tttcgcgcac caggatgcga aggccattcg ccgaactccg
     2581 aagccacgcc tcggtgtcga cggcgtcgtc ctcgcgacta gaaaagcgaa gcgcgtgacc
     2641 gacgaaccaa gccgccgtct ttgcggcgag ctgctccgac ccggccatgc ccacgaatac
     2701 ggcgctcact tcgagcgtcg cgagttcatg ctctgcgttc gcggcggcag ccatctcggc
     2761 cgcagcggtc gcgcttgacg ctgcccaggc gagaccgatg gccgcccaca cccccagctt
     2821 gcacaccgaa agcccgtgct tcactcagtc gctcgttcgt agccgtcgct gcgccggcag
     2881 ctcgtacgcg gagttcggat agcggctcac gagctccgac aacaccgcgc gctcttcgtc
     2941 gactcgaccg agttttcgca gcgcgttcgc ttcgccccac agcgattcgg gctcgaaacc
     3001 gccgccaccg gccgtgcgat gcgcccgaaa ctgctcgagt gcgagcgctg cgttgccttg
     3061 caatagatac aagttgccca tcgtgagatg cgacgcgacg gcctcccgtg agacggggta
     3121 gctagcctgc agctcgcgat acgtctcgag cgcgtccttc gtgcgacctt cttgtcgcgc
     3181 gcgattggcg cgctggtaaa gctccgccgc agaaacgttc gccgttgatg gcgccggcgg
     3241 cagttccgcg ggcgtgcttt ggtcggaaac cgaaacggcg ggcgcaagcg ccttcggcgc
     3301 gacaagcgaa ggctgcgcga gtggccgcac cggcgcaaac ccttcggcag atggaagcgc
     3361 gcgaaccttc gagccgctga gagcgggcga gactgtgtcg gcgggcaccg gcgcggcgag
     3421 gagcgcgggc gcatcgtctt ctggcgaagc aggcacattg ttgcgtgaag gcgctgcctg
     3481 cggcgctttg gctcgctcgc gatcacgttc cggcgtcgtg ctccgcgccg atgcggccgg
     3541 agctggggtc accgcactgt acacggcgat ggcacccacg cctgcaaaag ccccgcaagc
     3601 gaagcagatc gcggcggcga gcgccggcgt cgctcgcagc gcacgaccaa ctcgattgga
     3661 atgctgcggc cgcgccttcg cggcggcgag cgctggagta gctcgcagct cgcgcccgac
     3721 tcgattggcg cgctgcggcc gcgtttgcgt cgacgacgcg ctcgaccctt gcaatgcagc
     3781 gagctcggcg cggcgggcca tgcgcgtgat gagcgcttcg tcgtctggat gcgctgccgt
     3841 ttgacgatcc gcttcgagac cggcctcgag aaaacaccgc gcgccggcat cttcctcgag
     3901 cagcgccgaa agctcttcct cctcgaccgc cgtcaagggc tgacgccgcg cgcgcgccaa
     3961 caagtcgaac ggctcacgcg acctcatggg ctctcctctc cccgctcgag ccagagctcg
     4021 ctgccccgcg gatccgccag catacgcgtg cgcagcgccc ccttcgccaa gcgaagccga
     4081 ctcttccacg tctcgagcgg aataccactc gcgtcttgca tttcggcgag cgtgtagccg
     4141 agcacgacgt gtaaggcgag cgcctcggcc tgccccgccg gcaacgtggc gagaagctca
     4201 cggatcacgc ccaaactggc gcgtgcggca acctgccgtt cgggacctgg atcgctcgcc
     4261 gcgaacgaat cgacctcggc gccgagatcg cgctccctcg ctcgcttctg cgcccgctcg
     4321 cggcggcgtg cgttcaacgc cgtgagcaac gtgatgcgcc gcacgaaatg gaccaccgcg
     4381 cattcgccac gaaacgtcgc gagcgcatcg atcaaggcaa acgaacattc ctgcgcgacg
     4441 tcttccacgt cgggatcgcg cggccccaaa accccgcgca cgacgcgcac gacgtgaggc
     4501 cccaccgcaa gcaagaagcc tcgcacggct tcgggtttgc gtgccgccgc agccaccgct
     4561 agctcggcca aaggatcgac agcgggagac tccgctcgcg acgaacctct gccgccagag
     4621 agcactgcga atctgcgagg aggagaagga gcggacaccg ggtcgtttgc agtagttgta
     4681 gggcagccga gaacgggtca cggactttgc tcttttttcg atttcgcgga agcttccggc
     4741 ccgcaggtcg cttccagagg ctgaccaatg ccttcctgac ggcttcttgg ttgtccgcac
     4801 gcgacgcctc gcctatgcaa ccctcatgca actcgtccac gtttacctca atttcccggg
     4861 caccgcggca gcggcgatga agttctacga aggggtgttt gggacgaaag tcgccatgat
     4921 gcagaccttc ggcgaggcgc ccttcatgaa gggcacgccc gagagcgcaa aggacaagat
     4981 cctcca
//
LOCUS       DASTKS010001128         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_625216, whole genome shotgun sequence.
ACCESSION   DASTKS010001128 DASTKS010000000
VERSION     DASTKS010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625216"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VFQ35_23810"
     CDS             complement(<1..1266)
                     /locus_tag="VFQ35_23810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933759.1"
                     /translation="MSEPPPGYSAWLLAGFSSFLVFAAFVAYAFLAPAVYRTSALVEV
                     TRRPNTTAPNVAPVQATRRIHEAVLDREALVKLSEQRAGDTDARNQLASHLESALQID
                     SVDARTFVVSFRDSDRRRVTSTCNWLAERVAERAPRALAATASPANGERDRRRKLATF
                     LASHPELAPDAKTLLAHKALLELLASKEQLDNRALTERVAQLKTALDARIGELSAKRG
                     DSAKAEVKALGELQRTLSEMARGSDATAEPPFEARVASVASVPTWPIEPDRVRLLVSG
                     FLCACATGAIAGLIAQRRRDYDESIAGSSYPPEGSSSSPPPGTDSDADALDGPPSAET
                     TEGVPLQPWQAPNSVRPANSNPAPPNSAPSNPAPSGPNPIGANTPSGPNPIGANPIGA
                     NTPSGPNTPSGPNPIGANTPSGPNTPSGPN"
     gene            complement(1366..>2888)
                     /locus_tag="VFQ35_23815"
     CDS             complement(1366..>2888)
                     /locus_tag="VFQ35_23815"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9933760.1"
                     /translation="LRPPARGSAPEVMSESTLESAFSGSNAIERPLCVDLDGTLITTD
                     TLWESVASMFRKRPWTALALPLWLLGGRARFKHAIATRTTLDPASLPYRRELVEALRV
                     SRERGRKLVLATAADRKIADGVARHLGLFQAVHASDGQVNLKAAHKRDLLRSTYDQGF
                     DYVGDSGADEVVFAAATQGFLVGASRRAARIPERLPKVSLVSRKPSVVRALVKQLRPH
                     QWVKNALLFLPLLLAPEIPQLDQVGRAFLAWAAFCGCASAGYVFNDLLDIEADRAHAT
                     KRNRPFASGALPIIIGPPLFVGLMTLSLGLSAALLPWGFLAALVLYFAGTLSYSLWLK
                     KVLLLDVLVLAALYTHRILSGGAATGVRVSAWLLGFSLFIFTSLAFAKRYVELKAMTS
                     DDKVRNRGYFRTDLEMVTAMGIASGYTAALVFALYIDSAAVRTVYREASVLWLIMPVL
                     LYWFGRIWLLAGRGRMQEDPVKFAVKDPISLVCGGIIAGIAVLARFAPPFLVEIFH"
BASE COUNT          497 a          900 c         1033 g          458 t
ORIGIN      
        1 gttcgggccg ctcggtgtat tggggccgct cggggtattg gcaccgatgg ggttggggcc
       61 actcggggta ttggggccgc tcggggtatt ggcaccgatt gggttggcac cgattgggtt
      121 gggaccgctc ggggtgttgg caccgatggg gttggggccg ctcggggcag ggttcgaggg
      181 cgcagaattg ggcggagcag ggttggagtt ggcggggcgg acgctgttcg gcgcttgcca
      241 cggttgcagc ggcacgcctt cggtcgtttc tgcgctgggt gggccgtcca gggcgtccgc
      301 gtccgagtcg gttccgggcg gcggcgacga cgaggagcct tcgggaggat acgacgagcc
      361 cgcgatgctt tcgtcgtagt cgcgacgccg ctgcgcgatg agaccggcaa tcgcgcccgt
      421 cgcgcaagca cagaggaagc ccgataccaa gaggcgcacg cgatcgggtt cgatgggcca
      481 agtcggcacg ctcgcgacgc tcgcgacgcg cgcttcgaac ggaggttcgg cggtggcgtc
      541 gctgccgcgc gccatctcgc tcaacgttcg ctgcagctca ccgagggctt tcacctcggc
      601 cttcgcgctg tcgccgcgct ttgccgagag ctccccgatg cgcgcgtcga gcgcggtctt
      661 cagctgtgcg acgcgctcgg tcaaggcgcg attgtcgagc tgctccttgc tggcgagcag
      721 ctcgagcaat gccttgtggg cgagcagagt cttcgcgtcg ggcgcgagct ccgggtgcga
      781 agccaagaac gtggcgagct ttcgccgccg atcgcgctcg ccgttcgctg gcgacgcggt
      841 tgcggccaag gcacgcgggg cacgctctgc gacgcgctcg gcgagccagt tgcaagtgct
      901 cgtcacgcgg cgtcgatccg aatcgcggaa cgagaccacg aaggtgcgtg cgtcgacgct
      961 gtcgatctgc agcgccgatt ccaaatggct cgcgagctgg ttgcgcgcgt cggtgtcccc
     1021 ggctcgctgc tcggagagtt tgacgagcgc ctctcgatcg agcaccgcct cgtgaatgcg
     1081 acgggtcgcc tgaacggggg cgacgttcgg cgcggtcgtg ttggggcgcc gcgtcacttc
     1141 gacgagcgcg ctcgttcggt aaaccgccgg cgctagaaac gcgtaggcga cgaaggcggc
     1201 aaacaccaag aagctggaga aacccgccaa caaccaggcg ctgtaaccag ggggcggttc
     1261 gctcactcca cttggcggca tcggttcggc aaatgctccc gatgaacggt tgcgatcacg
     1321 caacagccta tcgcgctcat agcgcgaagg aaacgccgcg tggggtcaat gaaagatctc
     1381 caccaagaaa ggtggcgcaa agcgcgcgag caccgcgatc ccggcgatga tcccgccgca
     1441 aacgaggctg atgggatctt tgaccgcgaa ctttaccggg tcttcttgca tgcgcccgcg
     1501 cccggccaat agccagattc ggccgaacca atagaggagg accggcatga tgagccacag
     1561 gacggaggcc tcgcggtaca cggtgcgcac ggcggcgctg tcgatgtaca gcgcgaacac
     1621 gagcgccgcg gtgtagccgc tcgcgatgcc catggcggtc accatctcga gatccgtccg
     1681 gaagtacccg cggtttctca ccttgtcgtc gctcgtcatg gccttcagct cgacgtagcg
     1741 cttggcgaag gcgaggctcg tgaagatgaa gagcgagaaa ccgaggagcc aggcgctcac
     1801 tcgaacgccc gtcgcggcgc cgccggaaag gatgcggtgc gtgtagagcg ctgccaggac
     1861 gaggacgtcc aagagcagca cttttttcag ccaaagtgaa tacgacaggg taccggcgaa
     1921 atagagcacc agcgctgcca agaaccccca aggcaagagc gcggcggaga ggccgaggct
     1981 gagcgtcatc aaaccgacga acagcggggg gccgatgatg atgggcaagg cacccgacgc
     2041 gaatggccgg ttgcgcttgg tcgcgtgcgc acggtctgcc tcgatgtcga gcaggtcgtt
     2101 gaaaacgtag ccggcggagg cgcacccgca aaacgcagcc cacgcgagga aggcccggcc
     2161 gacttgatcg agctgcggta tctccggcgc gagcagcaac ggcaggaaca aaagcgcgtt
     2221 ttttacccac tggtgcggcc ggagctgctt gaccagcgcc cgaaccacgc tcggctttcg
     2281 ggaaacgagc gagaccttgg ggagccgttc cgggatgcgc gcggccctgc gcgaagcgcc
     2341 gaccaggaag ccctgagtcg cggctgcgaa cacgacctcg tcggccccgg agtcaccgac
     2401 gtagtcgaag ccctggtcgt aggtggagcg taacaagtcg cgcttgtgcg cggccttcag
     2461 gttgacttgg ccgtcgctcg cgtgcaccgc ctggaacaag ccgagatgtc gggcgacccc
     2521 gtcggcgatt ttgcgatccg ccgcggtcgc gaggacgagc tttcgaccac gctcccggct
     2581 gacgcgcagc gcctccacga gttcccgccg gtacggcaga ctggcggggt cgagcgtcgt
     2641 gcgcgtcgcg atggcgtgct tgaagcgcgc gcgaccgccc agtaaccaga gcggcaaagc
     2701 gagggcggtc cacggccgct tgcgaaacat cgacgcgacg ctctcccaca gggtgtccgt
     2761 ggtgatgagg gtcccgtcca ggtctacgca gagcggccgc tcgatggcgt tcgacccgct
     2821 gaacgccgac tctagcgtgg attcactcat gacttccggg gcgctgcccc gcgcgggcgg
     2881 tcgaagac
//
LOCUS       DASTKS010001129        11723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1297210, whole genome shotgun sequence.
ACCESSION   DASTKS010001129 DASTKS010000000
VERSION     DASTKS010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11723
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297210"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1368
                     /locus_tag="VFQ35_23820"
     CDS             433..1368
                     /locus_tag="VFQ35_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933761.1"
                     /translation="MPRAAKRYAGNDPTLRRLVREKCLGEFKEHFARDWDEHWQLGPS
                     LRSGGIAATEARAKQVVAKLRALIAEARQLTTWTPPSERPVDCAPPPTWARGGEAFAF
                     LLEWRPPAFLLPQVPDPVTADRERAIKDGQLVPLLGPVERAFDASEPPPLPRDDRDRV
                     VALWTSVNMWDGGSDGLLAPPELSFGKPARWLTIRELAIVTLLAGCWPAVRRLVTVAE
                     LIGCEEKFVRKALEKGFGTFWAGKRTQPEAADRVRSRERPTGSALTRHAAGRSTKRAA
                     AARARRGHETNREDDQSGLQSRRSRPRKHTRARRR"
     gene            1416..1679
                     /locus_tag="VFQ35_23825"
     CDS             1416..1679
                     /locus_tag="VFQ35_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933762.1"
                     /translation="MSRAEVAPVLLGPRNCEAACGMPWRHVRDTARALGVELVHVGRK
                     LAVPAAAFLEALAKKPAALAEPANDPVDGAAAVRALLGKRRRA"
     gene            1831..2745
                     /locus_tag="VFQ35_23830"
     CDS             1831..2745
                     /locus_tag="VFQ35_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933763.1"
                     /translation="MNELSDEQRAEIAAAKARGCSHCGRVIPQDERARCYRRLDVGFD
                     VVCVPCLEKEQARHAEEEKRRDEAERRRRLREFDQSFSDSCVGAELAKQENLVNSYLV
                     GWEGADVDTKTSSVGPLRFPKWKWARFENAAFRQRADARVLEVVEDYDCERDGSLVLC
                     GKTGSAKSSSVVAWIWQEHDALRTRVAAGEKLRFDFAWVSGFELAGARKRSQLGDEAP
                     LVKHASAVALLVLDDVGCEPKSEETFVVLDARYRAQLATIVTSPLEPAALIGHLGGGG
                     YRRALEHGRLVEAFGEAPKAKAGVRVVR"
     gene            3215..3694
                     /locus_tag="VFQ35_23835"
     CDS             3215..3694
                     /locus_tag="VFQ35_23835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933764.1"
                     /translation="MAPGKPVHSFALEAPKPKRKKAEQQTDPNVNVLRDWHRSEHARI
                     RGAEPVYTSNQHAAAGKSWKELLSIRSVDEAKAIITAALTEGFNVDPWAILKAQNKLA
                     TGASPRGGRNYQPQAGSPELLALGRGKTRAADLLPDQLARIREQEAIEARELEEALS"
     gene            3894..4505
                     /locus_tag="VFQ35_23840"
     CDS             3894..4505
                     /locus_tag="VFQ35_23840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933765.1"
                     /translation="MSERSHGALRPSLPRSSDLSVPQPPPDPRQGRDERGRFAPGNAS
                     GRGRGWKRAIARLAGSALAPEHRRLAGDAASLYRAVLAELPHGGPLVSPLVAEHSRCA
                     VLAPRFAERALELGLDTEAGRAALEVSMRLGQRAERVAISMLDLSTKLADARRRNRKN
                     TLATLLAGGEEVEDEPDTTVDPSENENASAWRTGAGDGDEQCT"
     gene            4486..5712
                     /locus_tag="VFQ35_23845"
     CDS             4486..5712
                     /locus_tag="VFQ35_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933766.1"
                     /translation="MTSSARDEAVHSPGHAPIADAEPRVHGAPEPDTRGEALGDGTLG
                     RRRADVAALAAGSGEAGEREADRGRADGDSGGAGNAGNGGGSVSADPPRLYDLVLAVA
                     QQMAQSVGDQLGDVLRRDKSKSWLFGAVRKRAGGRHGDSEIHDAIAALARQHKPAISD
                     ELGARAVSLSSRRQSVFHEPSDFPAWGPRVEQLAGLLNGEPVEVPALFELTRTRLGWE
                     NPNWTKNVLAAAENAGALHYHRRSSRWARQPELDEQDMAVLPPRAATGQQEVAFRSCG
                     APPAIAVQVRGRPRPRPRRPPRRANHPWRTKLMTTASPTPEKTNGKQNGAGATSTPPA
                     ASAEKGAGAERADSKPHKRPGPKPGTPRPKARAKPPSFEDELERALTPSLERVVRNVL
                     GRTLGKLVAEAVGGAK"
     gene            5712..6602
                     /locus_tag="VFQ35_23850"
     CDS             5712..6602
                     /locus_tag="VFQ35_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933767.1"
                     /translation="MFPPVASLSGFVLTFFRVLGATVSAAPKDTSDASNAAVLLGLEG
                     SVNDLDDPDGDTPGEQIPAVEAYGALGIVARPRQPEKIGGRDYAAEGYGGRTPNGMVP
                     FAYRDLRLNRRFPNPKPGSIALVGYGGGFLSFDDADGDTSLGTLYVPYARNGSGAVSK
                     AHMISIGKDGNGTPFVGIISGEGPRVTLLGNQVVVASKDGSNRLEVTDDGVNAVGPFK
                     AASGADLGGPTSREVVMFLELSNYVTQVNTLLSAMAAQLMAPGPVVGAAGAYTASLAA
                     ITAMTAALSSTGKSIFTKVA"
     gene            6602..6919
                     /locus_tag="VFQ35_23855"
     CDS             6602..6919
                     /locus_tag="VFQ35_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933768.1"
                     /translation="MSCALPPLGFPAVPGPSLPSPGVPNVPGIPAPPGGLPGIPSLGF
                     PAIGGPALPSAGVPTLPGVPVPPVNLPGTPSLAFPGIPGPALPLPGVPTVPGIPEPPC
                     PFS"
     gene            6916..8364
                     /locus_tag="VFQ35_23860"
     CDS             6916..8364
                     /locus_tag="VFQ35_23860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933769.1"
                     /translation="MTKRTKVTAFGGPGLTAQQRRRALELYDAMDRALGAAGFPATSP
                     WWRAQLARYLGSGRRRWIIRAGRRAGKSSMMCRLAVAVALWGAWSVPPGDLAVIPFVS
                     VDRNEASARLRTIREILTALDVDFDERGDEIELKDRRLAFRVVTGSVRGTVGFTSVLV
                     IADEMAKWESRDTAANPSREVMASLRPTMATQPTAFEVCSSSPWGTDDLHAELFDAGD
                     TDHQIVSFAATWTANPTITEEQTRELEPDPRIWSREYAAEPGATLSAALDLADLSAAF
                     GRQPVGELGPGFVAIDASSLRGDAFPFIAGRVTTAREFVILEVGGWSGAELRSVSMND
                     IVAKISERARAWKTSTVFGDQREEASLRSMFAKAGVSFISYAWTEPSKDEAVMLLRRM
                     LRDSTLHLPEHEVLRRELTTLKARLLPSGRTRYETNGFDYASALITLMHAAAAGHTPY
                     VHMESCAQPQNVRRRMLAGGWSPSSERTRQVY"
     gene            8380..8637
                     /locus_tag="VFQ35_23865"
     CDS             8380..8637
                     /locus_tag="VFQ35_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933770.1"
                     /translation="MPISDLHINRLEQRAHDARVNADLAGDELAKLERTLDTRRRAKL
                     AEIQALDDELEAARTHKTSLETEAQKLHDEWQANVPTETKE"
     gene            8644..9015
                     /locus_tag="VFQ35_23870"
     CDS             8644..9015
                     /locus_tag="VFQ35_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933771.1"
                     /translation="MNTADTANTPLERLKSYWDSPAHAELRDAAYLSDPMGSAAAQAE
                     FAQKMLDDAKGRLAAAKSGNPRPPSVAASARLIASYPGVNDVQRAIAYLSEKQPGFAK
                     LDWGRQNFLADRFLATGRASE"
     gene            9012..9893
                     /locus_tag="VFQ35_23875"
     CDS             9012..9893
                     /locus_tag="VFQ35_23875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933772.1"
                     /translation="MIQLQEPFRVSRPGEAFQRESETRQAATDKASAEARDYVGAHAR
                     EQQAIAEAERRTAEGELDARVRAAIDERKLSFASPEVPGTGWASSELRRTIAAAAWDA
                     RLHFTTLVHAEREIEVAKKELGATLASPILSRPASRLTELREQLDLCEARFEYALGVF
                     KEHCARLRDPAFVAMLDELNACAETARFTPYVTAGDPEMTVVANAVRALHDACSRVAQ
                     RQAAKLQEHSHATRLAKELGFTREQIGEESYRSIQPWLGQSTVQRAAKAAADAWRREH
                     GELDISGWLPNWDSPYG"
     gene            complement(9899..10195)
                     /locus_tag="VFQ35_23880"
     CDS             complement(9899..10195)
                     /locus_tag="VFQ35_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933773.1"
                     /translation="MSSASLSSFSRPSDPPRLEEKLLNRLRPRVSSAPVHTPEPVIYV
                     DVAGKRWNARTLERPRDGEPTRLLVMHGRSLCVMTVRRSDVHEGATWHYPPKPS"
     gene            complement(10796..11269)
                     /locus_tag="VFQ35_23885"
     CDS             complement(10796..11269)
                     /locus_tag="VFQ35_23885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933774.1"
                     /translation="MGYAPGLMAAYVVTAVFCERLLNEKDGTASLVRIIDRVHHEATA
                     IDTATRVESTSSEALAAVEGAPFLHQMVFYVSLWVDAPGTHQVDVTIAYEDGPRFEVA
                     SAGIEFSEPRQGRNMAVNIDGFPRPGDLWADVAIDGVLKTRTPLTLRRELGTLAQ"
     gene            11393..11602
                     /locus_tag="VFQ35_23890"
     CDS             11393..11602
                     /locus_tag="VFQ35_23890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933775.1"
                     /translation="MTGKPIPIRLEEDILARLEELAEAMSRRTGGVEITRASALRAAI
                     LIGLDKLEQENGIARRQSSTKTKRK"
BASE COUNT         2069 a         3987 c         3990 g         1677 t
ORIGIN      
        1 gcccgacgaa cgtcgcaagc gatttcacct tcagcggcac gggtcgcaaa gcgccccgtc
       61 atgcggacgg ccacacaccg tcgccatctt ttttcttcgt gggtcgactg ggactcgaac
      121 ccaggaccaa cggattaaaa accccgagcc ggattttgaa aatcgcggcg aagccgaaaa
      181 cggctcgggc cacgaacgag cagcgacgcc gaacgccgtc gatggggcaa tcgcggggca
      241 atcagcggcg gccgaggacc cggtagagtc agcgctcgcg cgcgcgatca cactcgcttc
      301 cgaggccggc cagtgggacg tcgtcacgac actcggccgt gagctcgggg agcgccggcg
      361 ggcgcgcacg acgccggcgg tagagtcgct tgacgaggtg cgcgccaggc gtggccggcg
      421 gtaggcttcg acgtgccccg agctgcgaag cgctacgcag gcaacgaccc gacgctccgg
      481 cgtctggtgc gtgagaagtg cctcggcgag ttcaaggagc atttcgcgcg cgactgggac
      541 gagcactggc aactcgggcc gagcctgcgc agcggaggga ttgccgccac agaggcgcgg
      601 gcaaaacagg tagtcgccaa gctccgggca ctgatcgccg aggcacggca gctgaccacc
      661 tggacaccgc cgtccgaacg ccctgtggat tgcgcaccgc ctccgacgtg ggcgcgcggt
      721 ggagaggcct tcgccttttt gctcgaatgg cgcccgcccg catttctcct cccacaggtt
      781 ccggatccgg tcaccgccga tcgtgagcga gccatcaagg atggacagct cgttcctctg
      841 ctcggccctg tcgagcgagc cttcgacgca agcgaaccac ctcccctgcc ccgtgacgac
      901 cgcgatcgtg tggtcgccct ctggacgtct gtgaacatgt gggacggcgg gtccgatggg
      961 ttgctcgctc ccccggagct atctttcgga aagcccgccc ggtggctaac gattcgcgaa
     1021 cttgcgatcg tcactctcct cgccgggtgc tggccggcgg tgcgccgact cgtgaccgtc
     1081 gccgagctca tcggctgcga ggaaaaattc gtccgcaaag ccctggagaa aggctttgga
     1141 acgttctggg cgggaaagcg cacccaaccc gaagctgcgg atcgggtgcg ttcacgagaa
     1201 cgcccgacgg gtagtgctct tacccgtcat gcagcaggtc gctctaccaa acgcgcagca
     1261 gcggcgcgag ctcgccgcgg ccacgaaacg aaccgagaag acgatcagtc gggtctacaa
     1321 tcccgacgct cgcgtccacg caagcacact cgcgcacgtc gccgctgagg cgcagcgcct
     1381 cgggtgtccg ctccctccgc cgcgcgccga ggcgcatgtc ccgcgctgag gtcgccccgg
     1441 tgctgctcgg cccgcggaac tgcgaggccg cctgcggcat gccgtggcgc cacgtgcgcg
     1501 acacggcccg cgcgctcggc gtcgagctcg tgcacgtcgg gcgcaaattg gcggttcctg
     1561 cggcggcgtt cctggaagcg ctcgcgaaga agccagcggc gctggcggag cccgcgaacg
     1621 acccggtcga cggcgccgcc gccgtgcgcg cgctgctcgg gaagcggagg cgcgcgtgac
     1681 ggaccgcgac gacgaggagt ccgagtgcgc gcgctgcgga ctgctcgcta gccgctgcct
     1741 gtgccgccgc cgtcatgaac caacgcgtga agagctcgag gaagcgcgcg agcagcggct
     1801 gacgcgcgcg gagcggggcc gtgacggctc atgaatgagc tttcggacga gcagcgcgct
     1861 gaaatcgcag ccgcaaaagc ccgcggctgt tcgcactgtg gacgggtcat tccgcaggac
     1921 gagcgcgcac gttgctaccg gcgcctcgac gttggtttcg acgttgtctg cgtgccgtgc
     1981 ctcgagaaag aacaagcgcg tcacgctgaa gaggaaaagc gtcgtgacga ggctgaacgg
     2041 cgccgacgat tgcgcgagtt cgaccaatcg ttttccgact cgtgcgtcgg tgcggagctc
     2101 gccaagcagg agaacctggt gaactcctac ctcgtcggct gggagggagc agacgtcgac
     2161 accaagacat cttcggtggg acctctgcgc tttccgaaat ggaaatgggc gcggttcgag
     2221 aacgccgctt tccggcagcg cgccgacgcc cgcgttctgg aagtcgtcga agactacgac
     2281 tgcgagcgcg acgggtcgct cgtgctgtgc ggcaagacag gatcggcgaa gagttcgagc
     2341 gtcgtcgcat ggatctggca ggagcacgac gcgcttcgga cgcgggtggc ggccggcgaa
     2401 aaacttaggt tcgacttcgc atgggtgtcg gggttcgagc tcgctggcgc gcgcaagcgc
     2461 tcgcagctcg gtgacgaggc gccgctcgtc aagcacgcga gtgcagtggc cctcttggtc
     2521 ttggacgacg tcggctgcga gcccaagagt gaggagacct tcgtcgtcct cgatgcacgc
     2581 taccgagcgc aactcgccac catcgtgacg tcgccgcttg agccggccgc gctgatcggt
     2641 cacctcggcg gcggcggcta tcggcgcgcg ctcgagcacg gtcgcctcgt cgaagcattc
     2701 ggcgaagccc cgaaggcgaa agcgggcgtg cgtgtcgtcc gatgaaaacg ggaggctccg
     2761 caagttggaa gctctgagcg ccatccgaga ttcgcgcact cacacgaagg ctgagaagct
     2821 cgtgctctac acgctgctgt cccacgccaa ggcgtcggac ggctcctgct atccgagcct
     2881 cgacacgctc gcggaggaat gcgggatggc ccgcagcgtc gcagcggcag cggtcgcgag
     2941 cctcctgaaa cggcaggacg gtccgctcct cgtgactgct gcgcaccgag gtcggacgga
     3001 cggctccgga ggccgagggt ccaaccgata tacgctccgg ttcagtccga caggcggacg
     3061 caagcgtcgc aaggaagagt ccggtgggcg gacgcaaccg gtcgcggact tgagtccgga
     3121 aaccgggggg gttgaatccg gaaattcggg acttgagtcc ggcgggcgga ctggaagctc
     3181 atcagagaag ctcgcaagcg aagaacgcca gtctttggca ccgggcaagc cggtgcattc
     3241 gttcgcgctc gaagctccaa agccgaagcg gaagaaagcg gagcagcaga ccgatccgaa
     3301 cgtgaacgtc ctacgggatt ggcaccggag cgagcacgca cgcattcgcg gcgcagaacc
     3361 cgtttacacg agcaaccagc atgccgcggc cggcaagtcc tggaaagagc tgctgtcgat
     3421 tcggagcgtc gacgaggcca aggccatcat cacggcagcg ctcaccgagg ggttcaacgt
     3481 ggacccgtgg gcaatcctga aggcgcagaa caagctcgcg acgggcgcgt cgcctcgagg
     3541 gggacgaaac tatcagccgc aggcgggctc gccggagctg ttggcgctcg gccgagggaa
     3601 aacacgcgcc gccgatctcc tgcccgacca gctggcgcgc atccgagagc aggaggccat
     3661 cgaagctcgc gaactcgagg aggctttgtc gtgaggcagg ccaacgaggc caacaggcca
     3721 aaggccaacg ctcctcgcgg cttgctcccg gaaacgcgcc ccggcgtggt gcgcgttgcg
     3781 cgcgctcggc gtttcccgac ggacttacga tccgttcgcg atcgcaggat tgccgcgcgt
     3841 ttcgcgatcg agccgtctga ttgccccgcg cgtcgccccc ggggagtcgc ccgatgagtg
     3901 agcgctcgca cggcgcgctg cgcccttccc tgcctcggtc gagcgatctt tccgtgccgc
     3961 agcccccacc cgatccgcgc caggggcgcg acgagcgcgg tcggttcgcg ccggggaacg
     4021 cgtctgggcg cggtcgcggc tggaaacgcg cgatcgcgcg cctcgccggc agcgccctcg
     4081 cgcccgagca ccgacgactc gcgggcgatg ctgcgtcgct gtatcgcgcc gtgctcgccg
     4141 agctgccgca tggcggcccg ctcgtttcgc cgctcgtcgc cgagcactcg cgctgcgccg
     4201 tgctggcacc tcgcttcgcg gagcgcgcgc tcgagctcgg gctcgacacg gaggcaggtc
     4261 gcgcggcgct cgaggtgagc atgcgcctcg gacagcgcgc cgagcgcgtt gcgatctcga
     4321 tgttggacct gagcaccaag ctcgccgacg cgcgccgacg caatcgcaag aacaccctcg
     4381 ccacgctcct ggctggcggc gaggaggtcg aggacgagcc cgatacgacc gtggacccgt
     4441 ccgaaaacga aaacgcgtca gcgtggcgca caggagccgg ggacggtgac gagcagtgca
     4501 cgtgatgaag ccgtgcactc gccgggtcac gcgccgatcg ccgatgcaga accacgcgtg
     4561 catggcgccc ccgaacctga cacgcgagga gaagctcttg gtgatggcac gctcgggcgt
     4621 cgccgagcgg acgttgcggc actggcagca gggtcgggcg aagccggcga gcgcgaagcg
     4681 gatcgaggac gcgctgacgg cgattcgggc ggagcgggca acgccggcaa cggcggaggt
     4741 tcggtaagtg ccgacccacc tcgcctctac gacctcgtgc tcgccgtcgc ccagcagatg
     4801 gcgcagtccg tcggcgacca gctcggggac gttctgcgcc gagacaaatc gaagagctgg
     4861 ctcttcggcg cggtgcgaaa gcgcgccggc ggccggcatg gcgatagcga aatccacgac
     4921 gccatcgccg cgctcgcgcg gcagcacaag cctgcgatct ccgacgaact cggcgcccgc
     4981 gccgtttccc tctcgagccg ccggcaatcc gtcttccatg aaccctctga cttccccgca
     5041 tggggcccgc gcgtcgagca actcgcgggc ctgctcaacg gcgagccggt cgaggtgcct
     5101 gcgctcttcg agctcacgcg cacgcgtctc ggttgggaaa atccgaactg gacgaagaat
     5161 gtgctcgctg ccgccgagaa cgcgggcgct ttgcactacc accgccgctc gtcgcgctgg
     5221 gcgcgccagc ctgaactcga cgagcaagac atggccgtgc tgcctcctcg ggcagcgacg
     5281 ggacagcagg aagtcgcgtt tcgtagctgt ggcgcgcctc cagcgatcgc ggttcaagtc
     5341 cgcggacggc cacgaccacg accgcgacgt ccgccaagac gagcaaacca cccatggaga
     5401 acgaaactca tgacgaccgc gtcccccaca cccgagaaga cgaacggcaa gcaaaacggc
     5461 gctggcgcga cgtcgacacc gcccgccgcc tccgccgaaa aaggcgccgg cgccgagcgc
     5521 gctgattcca agccgcacaa gcgcccggga ccgaagcccg gcacgccgag gccgaaggct
     5581 cgcgcgaagc cgccgagttt cgaagacgag ctcgagcgcg cgctcacccc gagcctcgaa
     5641 cgcgtcgtgc gcaatgtgct cggccgcacg ctcggcaagc tcgtggcgga ggcggtcggg
     5701 ggcgcgaagt agtgttcccc cccgtcgcca gcctctcggg cttcgtcctc acgttcttcc
     5761 gcgtgctcgg cgcaactgtt tccgcggcgc ccaaggacac ctccgacgcg agcaacgcgg
     5821 ccgtgctcct cgggctcgaa ggctcggtga acgatctcga cgacccggac ggcgacacgc
     5881 cgggcgagca gatccccgcc gtcgaggcgt acggcgcgct cgggatcgtt gcgcgcccgc
     5941 gccagcccga aaagatcggt ggacgcgact acgccgccga aggctacggc ggccgcacgc
     6001 cgaacgggat ggtgccgttt gcctaccgcg atctccgcct gaaccggcgg ttccccaacc
     6061 cgaagcccgg ctcgatcgcg ctcgtgggct acggcggcgg gttcctctcc ttcgacgacg
     6121 ccgacggcga cacgtcgctc ggcacgctct acgtcccgta cgcgcgcaac ggctccggcg
     6181 ccgtgagcaa ggcgcacatg atctcgatcg gcaaggacgg caacggcacg ccgttcgtcg
     6241 ggatcatcag cggcgagggg ccgcgcgtga cgctgctcgg caatcaggtc gtcgtcgcga
     6301 gcaaggacgg cagcaaccgc ctcgaggtga ccgacgacgg cgtgaacgcg gtcggaccgt
     6361 tcaaggcggc aagcggcgca gacctcggcg gccccacgtc gcgcgaggtc gtcatgttcc
     6421 tcgagctctc gaactacgtg acgcaggtga acacgttgct ctccgcgatg gcagcgcagc
     6481 tcatggcgcc cgggcccgtc gtaggcgcgg ccggcgcata taccgcgagc ctcgccgcaa
     6541 tcacggcgat gacggcggcg ctcagcagca ccggcaaaag catcttcacg aaggtcgcct
     6601 gatgagctgc gcgctgccgc cgcttggctt ccccgcagtg ccggggccga gcttgccgag
     6661 cccgggggtg ccgaacgtac caggcatccc ggcgccgccc ggcggcctac cgggcatccc
     6721 gtcgctcgga tttcccgcga tcggtggtcc cgcgctgccg agcgccgggg tgccaacgct
     6781 gcccggggtg cctgtcccgc cggtcaacct tccggggacg ccgagcctcg cattccccgg
     6841 catccccggt ccggcgctcc cgctgccagg cgtgccgacg gtgccgggta tccccgagcc
     6901 gccctgcccg ttctcatgac caaacgcacg aaggtcacag cgttcggcgg cccggggctc
     6961 accgcgcagc agcggcgtcg cgcgctcgag ctctacgatg cgatggaccg cgcgctcggc
     7021 gccgccggct tccccgcgac gtcgccgtgg tggcgtgcgc agctcgcgcg ctacctcggc
     7081 tccggtcgtc gccgctggat tattcgagcg ggccgccgcg cgggcaaatc gagcatgatg
     7141 tgccggctcg ccgtcgccgt ggcgctgtgg ggcgcgtggt cggtgccgcc gggcgacctc
     7201 gcggtgattc cgttcgtgag cgtcgaccgg aacgaagcga gcgcgcggct tcgaacgatc
     7261 cgcgaaatcc tgacggcgct cgacgtcgac ttcgacgagc gcggtgacga gatcgagctc
     7321 aaggaccgac gactcgcgtt ccgcgtcgtg accggcagcg tgcgtggcac cgtcgggttc
     7381 acgagcgtgc tcgtcatcgc cgacgaaatg gcgaagtggg agtcgcgcga cacagcggcg
     7441 aacccgtcgc gcgaggtaat ggcgtcgctg cgccccacga tggcgacgca gcccaccgcg
     7501 ttcgaagtct gcagctcgtc gccgtggggc actgacgacc ttcacgccga gctcttcgac
     7561 gcgggcgaca ccgatcacca gatcgtgagc ttcgctgcga cctggacggc gaacccgacg
     7621 atcaccgagg agcaaacgcg cgagctcgag ccggacccgc ggatctggtc gcgtgagtac
     7681 gcagccgagc ccggcgcgac gctttcggcc gcgctcgacc ttgcagacct ctccgccgcg
     7741 ttcgggcgtc aaccggtggg cgagctcggg cccggcttcg tcgcgatcga tgcgtcgagc
     7801 cttcgcggcg acgcgttccc gttcatcgcg ggtcgagtga cgacggcgcg cgagttcgtc
     7861 atcctcgagg tcggcggctg gtccggcgcc gagcttcgca gcgtttcgat gaacgacatc
     7921 gtggcgaaga tttcggagcg cgctcgtgcg tggaagacgt cgaccgtgtt cggcgaccag
     7981 cgcgaagagg cgagtcttcg aagcatgttc gccaaggcgg gcgtgagctt catctcctac
     8041 gcgtggaccg agccgtcgaa ggacgaggca gtgatgttgc tccgacgcat gctgcgggat
     8101 tcgacgcttc accttcccga gcacgaggtg ttgcgtcgcg agctcacgac gctcaaagcg
     8161 cgtctgctgc cgagcggacg cacacgctac gagacgaacg gcttcgatta cgcctcggcg
     8221 ctcatcacgt tgatgcacgc ggccgcggcg gggcacacgc cgtacgtcca catggagagc
     8281 tgcgcacaac cgcaaaacgt gcgccgtcgc atgttggccg gcggctggtc gcccagctcg
     8341 gaacggacac gacaagtcta ctgacgaaag ggaccaacca tgccgatcag cgaccttcac
     8401 atcaaccgac tcgagcaacg cgcgcacgac gcgcgcgtga acgccgacct cgccggcgac
     8461 gagctcgcga aactcgagcg cacgctcgac acgcgacgcc gcgcgaagct cgccgagatc
     8521 caggcgctcg acgacgagct cgaggcggca cgcacccaca agacgtcgct cgagacggaa
     8581 gcccaaaagc ttcacgacga gtggcaagcg aacgtcccca ccgaaacgaa ggagtgagac
     8641 accatgaaca ccgcagacac cgcgaacacg ccgctcgagc ggctcaagag ttattgggac
     8701 agccccgcgc acgcggagct tcgcgacgcc gcgtaccttt ccgatccgat gggcagcgcc
     8761 gcagctcagg cggagttcgc ccagaagatg ctcgacgacg cgaagggtcg gctcgcggcc
     8821 gcgaaaagcg gcaatccgag gccgccgtcg gttgcggcgt ccgcacgcct gatcgcgtcc
     8881 tacccgggcg tcaacgacgt gcagcgcgcg atcgcctacc tgagcgagaa gcaacccggc
     8941 ttcgcgaagc tcgactgggg tcggcagaac ttcctcgcgg accgctttct cgccaccgga
     9001 agggcaagcg agtgatccaa ctccaagagc cgtttcgggt aagccggccg ggtgaggcgt
     9061 ttcagcgcga gagcgaaaca cggcaagccg ccaccgacaa ggcgtccgcc gaagcacgcg
     9121 actatgtcgg cgcgcacgca cgcgagcaac aggcgatcgc cgaagccgaa aggcgaaccg
     9181 ccgaaggcga gctcgacgcg cgggtccgcg ccgcgatcga cgagcgcaaa ctctcgttcg
     9241 catcacccga ggtgcccggc accgggtggg cgtcgagcga gctccggcga accatcgccg
     9301 cggccgcgtg ggatgcccgt ctccacttca cgacgctcgt gcatgcggag cgcgagatcg
     9361 aggttgcgaa aaaggagctc ggcgcgacgc tcgcgtcgcc catcctgtcg cggccggcct
     9421 cgcgactcac cgagctacgc gagcagctcg acctctgcga ggcccgcttc gaatacgcgc
     9481 tcggcgtctt caaggagcac tgcgcgcggc tgcgcgatcc ggcgttcgtc gccatgctcg
     9541 acgagctcaa cgcatgcgcc gagacggcgc gcttcacgcc gtacgtgacc gccggcgatc
     9601 cagagatgac ggtggtcgcg aatgcggtgc gggcgttgca cgacgcctgc tcacgcgtcg
     9661 cgcagcggca ggcggcgaag ctccaagagc actctcacgc cacgcggctc gcgaaagagc
     9721 tcggcttcac acgcgagcaa atcggcgagg agagctatcg atcgatccag ccgtggctcg
     9781 gtcagagcac tgtgcagcgc gcagcgaagg cggcggcgga cgcgtggcga cgggagcacg
     9841 gcgaactcga catctccgga tggctgccga actgggacag cccgtacggg tgaccgcctc
     9901 agctcggctt cggcgggtag tgccaggtcg cgccttcgtg cacgtccgag cgccgcaccg
     9961 tcatcacgca gagcgagcgg ccgtgcatga cgaggagcct cgtcggctcg ccgtcgcgcg
    10021 gccgctcgag cgtgcgggcg ttccagcgct tgccggcgac gtcgacgtag atgacgggct
    10081 cgggcgtgtg cacgggcgca gagcttaccc gtggccggag ccgattcagg agcttttctt
    10141 cgaggcgcgg tggatcggaa ggacgtgaga aggacgagag cgaggcgcta ctcaccgttc
    10201 cacgcgtact caatgccttc tacgagcaga tactttggat ccggcaatcc gctcgccgcg
    10261 agagaaccgt cacgccgtag gtactgatgc acgagagcaa ccttcgtacc gtccgctcgc
    10321 aggtaggcaa ccgactgcga agtaacgact tcatcatcaa gctgccgcga gttagagcgc
    10381 agaacacgcg cggtgagctc cccccgttgc attcgttcgt agaaatggcc agtattgaac
    10441 agggttcgga gcactaccga gcccaccgaa cgccgaggcg atcctatcat gctcaccgaa
    10501 cacgacctgc ttcaagcagt gcgagcaacg ctcgcgggcg agcaaaccgt ggcgcagcta
    10561 gcacgctcgt caggcgaggc gctcgcggat catgtcgctg acctggagac gcctgtcggt
    10621 cgcctcgctg tggcgttggc gtcgctatgc acagagcttc atgatggcgc tgtcgacgca
    10681 accaacttcc gcacggagct tcaagcgctg cttcgtccgc agctcgtgcg ggcgcacgct
    10741 cgcttgcccg ttccgcggca gccggtttcg attgcaacgg gtgcatccgc cgggctcact
    10801 gagcgagagt accgagctcc cgccgcagag taagcggggt ccgcgtcttc agcacaccgt
    10861 cgatggctac gtcggcccaa agatcgccgg gccgcggaaa cccgtcgatg ttcacggcca
    10921 tattgcgccc ctggcgcggc tccgagaact cgatgcccgc cgaggctact tcaaagcgag
    10981 ggccatcctc gtaggcgatg gtcacgtcga cctggtgggt accaggcgca tccacccaga
    11041 gcgaaacgta gaacaccatc tggtgaagga acggcgcccc ctcgaccgcc gcgagcgcct
    11101 cgctcgaggt ggactccacc cgcgttgcgg tatcgatcgc agtagcctcg tggtgaacgc
    11161 ggtcgatgat tcggacgagg ctcgccgtcc cgtccttctc gttgagaagg cgttcgcaaa
    11221 agaccgcggt gactacgtag gccgccatga gacccggagc gtaacccact cgcggcaaga
    11281 ggcacacgtt gaggacgtcg gccggacttg cgcggaggat tcggccgtcc tccgggcacc
    11341 ttgacggctc gcttgcgctt caatgcggca cgatgtatac gagagtagaa gcatgactgg
    11401 taagccgatt cctatccgcc tcgaggagga catccttgcg aggctggagg aactcgcgga
    11461 agcgatgagc aggcgaacgg gcggtgtcga aatcacccgg gcaagtgccc tgcgtgccgc
    11521 gatcctcatc gggctcgaca agcttgaaca agagaacggg atcgctcgcc gtcagagcag
    11581 tacgaaaacg aaacgcaagt aagcgggcgc cccggaggta ggactccgag acgccccatc
    11641 accacggaag gtgtagttcc atggcaacga agaagacgat cgcacgaacg aaacgcgctg
    11701 tccctgcgac gcgcaaacag gcc
//
LOCUS       DASTKS010001130         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1968150, whole genome shotgun sequence.
ACCESSION   DASTKS010001130 DASTKS010000000
VERSION     DASTKS010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968150"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFQ35_23895"
     CDS             <1..1021
                     /locus_tag="VFQ35_23895"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9933776.1"
                     /translation="NRAQILPSESPQFGDVIGASLAMRSIFGLLERIAPTDACVLIEG
                     ETGTGKDVLARSIHQLSTRKDSEFVVVDCGAISYNLIESELFGHERGAYTGAVAARQG
                     AFELAGRGTVFLDEVGELPLDVQPKLLRVLESGEFRRVGGNKTMRTEARIIAATKRNL
                     REEVERGKFREDLYFRLSVVPVTVPPLRQRREDIPALVERFLDQARKRVPSAANITLS
                     RETLAALSAHDWPGNVRELRNVLDRAIYVAAAGGERDVRLVDVPVSATPIGSVQAPFP
                     AFEPGKSYREVRADFEADFERRYVSWLLDRHAGNISAAAREAKMDRKHLYDLARKHGL
                     RGERG"
     gene            complement(1100..2371)
                     /locus_tag="VFQ35_23900"
     CDS             complement(1100..2371)
                     /locus_tag="VFQ35_23900"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HET9933777.1"
                     /translation="MRGRLVHFAKGKSRFVVGCVGALSSFAVALAPAQAQVVSADLRT
                     NAFIEPSSSSKLIVINPAVDVAATPSDFLKINAGYQADIVSGASESVKAGRFAGVDVV
                     SAATSFHDTRHQASGGFSLIRDVTELDATYSYGTESDYRSHSIAVSAATNFLQRNTEL
                     RLSYARGFDRVCTSGFSSSDDPSVRAPLDSSKGCFTKTENRASRPIDIDSFQAGWTQT
                     WTPVIATQLNLTASLQHGFLGNPYRGVVIASAGEVAVENHPENRARAAASFKLRLYAR
                     PLRTAFGVGAHIYRDTWDVLAGTLSLDAERYLFPGFRVQARARYYAQSGALFYSDDYT
                     GGEPKDGPRGQYWTGDRELSPLQSYMLGGRLVYSKRGEPGARVLGALLGLSASLSLDL
                     MKTKLNDFTWGGKEPDDTFAFLGSLGLSGEF"
     gene            complement(2377..2625)
                     /locus_tag="VFQ35_23905"
     CDS             complement(2377..2625)
                     /locus_tag="VFQ35_23905"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET9933778.1"
                     /translation="MQKRSTSRSFWAICGSVAGAVAAASVAGGCATVRPEQRAILADP
                     IMQFEGDQSESAGREHVLENREAAIGSGSIKGGGCGCN"
     gene            complement(2638..3408)
                     /locus_tag="VFQ35_23910"
     CDS             complement(2638..3408)
                     /locus_tag="VFQ35_23910"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9933779.1"
                     /translation="MGDAVLKSVIALGLSALLLGAPRPASAADAEAFARVVVYETELR
                     SGPGVSYRVIHRARRGEAFLIKTRETKGYWLEVELADGRTAYVLGDTVETVGIDEEAG
                     EGGPKRFGIFAPPALAEAHGGFALLAGIYDRQGYAEIRPAWVIAPAIALEPYVGLALQ
                     SDSRRIMYGAAGTLNLAPDWPIAPFVQIGFGGVFEDPKDEFVRRSRKWFHSRAGGGLL
                     ISLRSRVLVRLEVSNVVLFGEDSYQNTQAYIAGLGTYF"
     gene            complement(3381..3890)
                     /locus_tag="VFQ35_23915"
     CDS             complement(3381..3890)
                     /locus_tag="VFQ35_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933780.1"
                     /translation="MIPPRFFARRALVVALLLVGCGEHGLLPTTVDPGPDFSVADVVF
                     NSGFYYCKVEPMLMEQRCGGGDPSKGDASNGCHATTTSFRFTEYMPPVADSCGGGVTP
                     GTVTIPQLAQQNYQGAQARMRRSPDGPPPAPLLARPTGKAQHPRVIFESMSAEADLIR
                     QWATQYSSQ"
BASE COUNT          750 a         1421 c         1365 g          687 t
ORIGIN      
        1 gaaccgcgcc cagatcctgc cgagtgagtc gcctcagttc ggcgacgtca tcggcgcgag
       61 cctcgcgatg cgcagcatct tcgggttgct cgagcgcatc gcgccgacgg acgcgtgcgt
      121 cctcatcgag ggggagaccg gcactggcaa agacgtcctc gcccgctcga tccatcaact
      181 cagtacgcgc aaggacagcg aattcgtcgt cgtggactgc ggcgcgatta gctacaacct
      241 catcgagagt gagctcttcg gtcacgagcg gggcgcttac accggcgcgg tcgccgcgag
      301 gcaaggcgcg ttcgagctcg cggggcgcgg caccgtcttt ctcgacgaag tcggggaact
      361 accgctcgac gtgcagccga agctgctgcg cgtcctcgag tcgggcgagt tccggcgcgt
      421 cggcggcaac aagacgatgc gcacggaagc gcggatcatc gccgcgacga agcgaaacct
      481 ccgcgaggag gtcgagcgcg gcaagttccg agaagatctc tacttccggc tctcggtggt
      541 gccggtaacg gtcccgcccc tgcgccaacg ccgagaagac attcccgcgc tcgtcgagcg
      601 cttcctcgat caagcgcgca aacgcgtccc ctcggccgcg aacatcacgc tgagccgcga
      661 aacgctggcg gccctgagcg cgcacgattg gcccggtaac gtgcgtgagc tccgcaacgt
      721 cctcgaccgc gccatctatg ttgccgcagc gggcggcgag cgcgacgtcc gcctcgtcga
      781 cgtccccgtt tcggccacac ccatcggcag cgttcaagcg ccgttccccg cgttcgaacc
      841 cggcaagagc taccgcgagg tgcgcgccga tttcgaggcc gacttcgagc gccgctacgt
      901 ctcgtggctc ctcgatcggc atgccggcaa catcagcgcc gccgcccgcg aggcgaagat
      961 ggatcgcaaa cacctttacg acctcgcccg caagcacggg ctacgcggcg aacgcggcta
     1021 gagccgcaga aaacggcggc cccgtgcgtg gttccgcgac ggtgctctcg gcagcggcca
     1081 cctaagcagg acggcgcggc taaaactcgc cgctcaaccc gaggctgccg aggaacgcaa
     1141 acgtgtcgtc gggctccttg ccgccccacg tgaagtcgtt gagcttggtc ttcatgagat
     1201 cgaggctcaa cgaagcagag aggccgagca aggcgccgag tacgcgcgct ccgggctcgc
     1261 cgcgcttcga gtaaacgagg cgtcccccga gcatgtagct ttgcagaggg gaaagctcgc
     1321 gatctcccgt ccagtattgt ccgcgcgggc cgtccttggg ctcgccgccg gtgtagtcgt
     1381 cgctgtaaaa gagagcgccg ctctgagcat agtagcgagc tctcgcctgc acccgaaagc
     1441 cggggaacaa atagcgctcg gcgtcgagtg agagcgtccc cgcgaggacg tcccacgtat
     1501 cgcggtagat gtgcgcgcca acgccgaacg cggttcgcag cggccgagcg tagagtcgca
     1561 atttgaacga cgccgccgcg cgagcgcgat tttcgggatg attttctact gcgacctcgc
     1621 ccgccgaggc gatgacgacg ccgcgatacg ggtttccgag aaagccgtgt tgaagggacg
     1681 cggtgaggtt cagctgcgtc gcgatgacgg gcgtccacgt ctgcgtccag ccggcttgga
     1741 agctgtcgat gtcgatggga cgcgaggcgc gattttcggt cttggtgaag cagcccttcg
     1801 agctgtcgag gggcgcgcga acgctcgggt cgtcggagct cgaaaacccg gacgtgcaca
     1861 ctctgtcgaa gccgcgcgcg tacgagagcc gaagctcggt attgcgctgc aagaagttcg
     1921 tcgccgcgct caccgcgatc gaatgcgaac ggtaatcgct ctccgtgccg tagctgtagg
     1981 tcgcatcgag ctcggtgacg tcgcggatca agctgaagcc gccgctcgcc tggtgacgcg
     2041 tgtcgtggaa cgacgtcgcg gcggaaacga cgtcgacgcc ggcgaagcgc cccgccttca
     2101 ctgattcgct cgcgccgctc acgatgtccg cctgataccc ggcgttgatc ttcaagaagt
     2161 cggacggagt cgcggccacg tcgaccgcgg ggttgatgac gatgagcttg ctcgaagagc
     2221 tcggctcgat gaacgcattc gttcgcagat cggcgctcac gacctgtgcc tgggctggcg
     2281 cgagcgccac cgcgaagctc gaaagagctc cgacgcagcc cacgacgaac cgactcttgc
     2341 ccttcgcgaa gtgaaccaag cgtccccgca ttcgcctcag ttgcagccgc acccgccgcc
     2401 tttgatcgag ccgctgccta tcgcggcctc gcggttctcg agcacgtgct cgcgaccagc
     2461 cgactcgctc tggtcgccct cgaactgcat gatggggtcg gccagaatcg cgcgctgttc
     2521 ggggcgcacc gtcgcgcatc cgccggcgac gctggcagca gccacggcac ccgccacgct
     2581 tccgcagatg gcccagaaac tacgactcgt ggatcgtttt tgcatgggtc acatctccta
     2641 gaaatacgtg ccgaggccgg cgatgtacgc ctgggtgttt tggtaactgt cttcgccgaa
     2701 gaggaccacg ttcgagacct cgagccgcac cagcaccctc gagcgcagcg aaatcaggag
     2761 ccccccgcca gcgcgcgagt ggaaccattt gcgggagcgg cgcacgaact catccttcgg
     2821 gtcctcgaag acgcccccga acccgatctg cacgaacggc gcgatcggcc aatcgggcgc
     2881 gaggttcagg gtccccgcgg cgccgtacat gatgcggcgg ctgtcgctct gcagcgcgag
     2941 gccaacgtat ggctcgagcg cgatcgcggg cgcaatgacc caagcgggcc tgatttcggc
     3001 gtatccctgg cggtcgtaaa tgccagcgag taacgcgaac cccccatggg cttcggcgag
     3061 cgcggggggc gcaaagatgc cgaagcgctt cggcccgccc tcgccagcct cctcgtcgat
     3121 tccaaccgtt tcgacggtgt cgccgagcac gtaagccgtg cggccgtcgg cgagctcgac
     3181 ctcgagccag taccccttgg tctcacgggt tttgatgagg aacgcctcac ctcgacgagc
     3241 gcggtgaatg actcggtacg agacgcccgg cccggagcgc agctcggtct cgtagacgac
     3301 gacccgcgcg aaggcctccg cgtctgccgc cgacgctggg cgcggcgccc cgagcaggag
     3361 cgccgacaac ccaagcgcga tcactgactt gagtactgcg tcgcccattg tcgaatcaga
     3421 tccgcttcgg cgctcatcga ctcgaagatg acgcgcgggt gctgagcctt gccggtgggg
     3481 cgcgcgagga gcggcgccgg cggcggacca tcggggctcc gacgcatgcg cgcctgagcg
     3541 ccttggtagt tctgctgtgc gagctgggga atcgtgaccg tgcccggggt cacgccaccg
     3601 ccgcagctgt cggcgacggg aggcatgtac tcggtgaagc ggaagctcgt ggtcgtcgcg
     3661 tggcagccgt tcgatgcgtc gcccttggag gggtcaccgc cgccgcagcg ctgctccatc
     3721 aacatcggct cgaccttgca gtagtagaag ccggagttga acacgacgtc ggcgacgctg
     3781 aagtccggtc ccggatccac cgtagtcggc aggagcccgt gctcaccgca cccaacgagc
     3841 aacaacgcca cgaccagggc gcgacgggcg aagaaacgcg gggggatcac gccccacaac
     3901 gctacaccgt tctagcctcc gaacgcgagc tcgatggcga ttttcctgag ccgtcccgcg
     3961 ggcttcgcac cttccggcaa gtttacgtac tctcaccttc atccacccac ataaataagc
     4021 gcgctccgcc acagactctt gaacgcgtcg tcgcggctcg gtaactcttt gccccgccag
     4081 cgagacctgt ggcaccatta cttacgttca cttacgttat cccacctagg gttcacttaa
     4141 cttccgtcga gtaagcaggg ggcttggctc gaaaaggcca attagccgtg gagaaccgcg
     4201 cagaatgaag ttcaacccct ggt
//
LOCUS       DASTKS010001131        14981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1072886, whole genome shotgun sequence.
ACCESSION   DASTKS010001131 DASTKS010000000
VERSION     DASTKS010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072886"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFQ35_23920"
     CDS             <1..681
                     /locus_tag="VFQ35_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9933781.1"
                     /translation="AAVKNFATYSLKRLGTEVIDVYRPARLDPNVPVEDTVGAIAELI
                     EAGYVKYVGLSEVGAQTIRRAHAVHPVADVQMEYSIATRTPEEKVFPVLHELGIGATL
                     FGVLARGLLSGSKPSSPKDVRARLPRFSGDAAKENEVLVERLRLFAAKAGRTPAQVCM
                     GWALAKEPAFVPVSGAHKPAQLDVLDALNLPLSADEMAELENLLPKGSFKGSRYPEAQ
                     MADLDSER"
     gene            complement(711..1088)
                     /locus_tag="VFQ35_23925"
     CDS             complement(711..1088)
                     /locus_tag="VFQ35_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9933782.1"
                     /translation="MTVQLNHTIVPSRDAKASAEFLSDILGLPAPRRFYHFWVVNTAN
                     GVSLDFLEVEGEIQSTHYAFLISEAEFDEIFARIEARGVPYWADPGAGLPQQINHDGG
                     GRGIYFHDPAGHFLEIITRPYGG"
     gene            1325..2119
                     /locus_tag="VFQ35_23930"
     CDS             1325..2119
                     /locus_tag="VFQ35_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HET9933783.1"
                     /translation="MPHRDVALSTAQVEQFIYDGFVRVDEAFPREVADACRRILWLAT
                     GCSEDAPATWTRPVVRIGEIPHPLFREAANTPRLHAAYDALVGPGRWLPRGSLGTFPI
                     RFPSAEDPGDCGWHVDTSFGSEGEPDFLKWRVNVASKGRALLMLFLFSDVGDDDAPTR
                     LRVGSHRDIAQRLEPHGEQGLSLGELASSGFTESAHRPEATATGPAGTVYVCHPFLVH
                     AAQRLHVGAMPRFLGQPALLPKVPLDPWRDDAFPVERAIRLALDAR"
     gene            2291..3295
                     /locus_tag="VFQ35_23935"
     CDS             2291..3295
                     /locus_tag="VFQ35_23935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9933784.1"
                     /translation="MRALVVGGTGLVSTGVVQQLLARGVRVSVSNRGTRSNSFGSDVR
                     LLRGDRANASEFVRTFEREQFDVVYDMICYSPEDAEVSARAFAGRCEQLVLCSSAIVY
                     GRRMPSSVLIDESAPLEPTSHWGKSKVLCEQALQRAADKGAFKLTIARLGHTYGPGDF
                     MNDQQDNDGTAWDRVVRGLPVFCTGDGLGLWQSTHRDDTAKFFAFAALAPQTYGEAFN
                     VMRDEILTWRDYYREVARSLDTTANLLFVSASWLIAQAPERFSFLAEISQFHAAYSSA
                     KAKAAVPEFRATIGLEAGARETFADTRRRGVWADSSRDTSYDELVDRALTLGFRVEAA
                     "
     gene            complement(3505..4341)
                     /locus_tag="VFQ35_23940"
     CDS             complement(3505..4341)
                     /locus_tag="VFQ35_23940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9933785.1"
                     /translation="MKPGTESQTAVMVAAARAAAHGRTAVGAFADPTALTLLPADARQ
                     RIERQLAGVPPSGLSERMQRRMVEGRVKMMVPRTVAIDRAIREAASPQLVILGAGLDG
                     RAWRMAELADTTVFEVDHPDSQRRKRERAASLKLAARELHFAPVDFTRDRLEDALVAA
                     GHDPARVTTWLWEGVVMYLAREDIEATLRVVRARSAPGSRLIVLYTSHSIVRKTAGLL
                     LGLIGEPFRSAFSADEMRTLLESHGFSVLHDDDLAHIASRLGGETSRLFKDVRLVSAE
                     LR"
     gene            complement(4352..5005)
                     /locus_tag="VFQ35_23945"
     CDS             complement(4352..5005)
                     /locus_tag="VFQ35_23945"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9933786.1"
                     /translation="MGRTTRPEPSAPALGLRERKKRALRGELTLAALRLAKERGIENV
                     RTEDIVAAVGVSRRTFSNYFASKYEALADRLVQRAQHSAKVLRARPRAETLWQALSAA
                     MVAPYENLVLNDSSESREATLLVLEDATLRAELWKGELAAQRALALAVAERTGLDSER
                     DMYPSIVAATAVAAVTTVLERWLRSKRPPPLIPMIREALELIERGLPEPARRRRAKR"
     gene            complement(5165..5818)
                     /locus_tag="VFQ35_23950"
     CDS             complement(5165..5818)
                     /locus_tag="VFQ35_23950"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9933787.1"
                     /translation="MKKAEPKRAESTLEFSHVGEWSEWLRSNHSHSPCVFLRIPKKGV
                     KNAITYAEALDVALAWGWIDSQKRALDSEAWLQRFGPRTAKSPWSKINRAKAEALIAA
                     GAMEAPGLAEVERAKRDGRWESAYDGARAASIPADLAAAFARSARARAFFETLDGANR
                     YAILYRVQTAKKPETRAARIERFVEMCARHETLHPPRPAKAATRATAKPQKARSAKK"
     gene            6057..6851
                     /locus_tag="VFQ35_23955"
     CDS             6057..6851
                     /locus_tag="VFQ35_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9933788.1"
                     /translation="MGTKATVGILNPFEGFKHFSLRREAPPEDLAPFVERFWSVRWDL
                     DGRPPFAQEILPYPCVNLSTGSGGFEVHGPATKRFVARLSGRGEVFGTKFTPAGFLVF
                     TRTPLRDLVDRIVSIEAAAGRGPCAPTTHDFDAVRPQVETFLRSFHPKPTEAMALADR
                     LVRLAQEDRSITRAEDLARTAGISVRSLHRLFEHHVGVGPKWVIRRSRVQEAAERVAR
                     GEPVDWAGAAQELGYHDQAHLIRDFREQVGATPAAYARQCGASVVG"
     gene            complement(6935..8260)
                     /locus_tag="VFQ35_23960"
     CDS             complement(6935..8260)
                     /locus_tag="VFQ35_23960"
                     /inference="COORDINATES: protein motif:HMM:NF025444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HET9933789.1"
                     /translation="MGTWGPGPFDNDAASDFLEQLHASPSRVVAKALRDVAKRPAGNY
                     IDVDDGGAAWAACELVALAFGYGDADAAGDAVLDMAARLTPKQEQRRLALEVLTRIAD
                     RTTSELAGLWHEGSDGAGFDASIAHLRTRLEAASSGARESPKAKKGDVIALGAASSSG
                     DMIVVQVVAPREVAVMMGTFVDDATALATVKHGPAQRLPAPVNELLRKGRVLGNVPVR
                     KDLRGKKLYAGEIGAITGYILATASAGGARVVSYEEACDRELLRPYGEDAIRAIALGK
                     KAIQRVRSPDEREAQLTARNATKWASRRDVTDPGPFGDVEIVEGLLKWIEDYGVENAI
                     RGFHDEARGVIGYGRPNEYPERRSYAFVGIVAIWCKVRSCDDWPSALHGRLPETPEKG
                     LLDRAVEAARMLVEEVVTREAELRMIWEAGPDRGAGLHAAVAALKSELS"
     gene            8484..8846
                     /locus_tag="VFQ35_23965"
     CDS             8484..8846
                     /locus_tag="VFQ35_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9933790.1"
                     /translation="MVQYSSDRLDASFAALSDATRRGVLDQLGRADASISDLAEKFKM
                     TLTGMKKHVGVLEQAGLVSTEKIGRVRTCRLGPRRLEEEAAWIERYRQLWAARFDELD
                     TVVDELKQKEKSNARKKR"
     gene            8827..9348
                     /locus_tag="VFQ35_23970"
     CDS             8827..9348
                     /locus_tag="VFQ35_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9933791.1"
                     /translation="MPERNDSAGTTTKNRTTTERKSERELVVTRTVNGPARLVFEAWT
                     KPELFQRWWVPKSFGLTLLSCEMDVRVGGQYRLVFKHEDSTMAFFGTYREVTPPSRLV
                     WTNEEGEDGETVTTVTFDEKDGKTLVVVHDLYASKEALDAAIASGSTGGLPEQLDELE
                     ALLESFGATPENG"
     gene            9345..9917
                     /locus_tag="VFQ35_23975"
     CDS             9345..9917
                     /locus_tag="VFQ35_23975"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9933792.1"
                     /translation="MTTRSPLRAKRAAAPKSKKAPAAKPHAKAAAKPRAKAAAKPHAK
                     AAAKPAAKLLVAMTGKASPAKAKEGDEPVFAYIASLPQPQRRIAERVDALAAKTLPNL
                     QRAVKWGMAYYGVDGGWCFSSGAFIGHLKLMFIRGTELEPEPPVTPIGMGKSTRGVEL
                     AAVNEFDERQLAAWMKQAATMPFVGGKKKR"
     gene            10132..10965
                     /locus_tag="VFQ35_23980"
     CDS             10132..10965
                     /locus_tag="VFQ35_23980"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9933793.1"
                     /translation="MSNEQMRWLLKVIQPARTLASLPGGAGLSQEAHAALLGLPPEGY
                     AAELGRMKAEAKEAASELLADPAIASMVDRLPLRKGATVAAFGDSLTSDPQSWAVILS
                     ELLSARRAADGISVAISAVGGDTTTHGLVRVGEVVAQRPDWIFFFIGTNDARTQGSHP
                     TKTLVHHEETMRNIAELRQRVARETKARAVWITPPAVNEEQVASHWGLSRFGVRFCNE
                     DLARVAKIVRDVDGAAIDVFSMLGTPPSADLLMGDGLHFTVAGQKRLALEVLKGWSNL
                     E"
     gene            10962..11813
                     /locus_tag="VFQ35_23985"
     CDS             10962..11813
                     /locus_tag="VFQ35_23985"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9933794.1"
                     /translation="MKYLIFGATGNIGSRVTQRLIARGERASVFVRNAKKAKALFGEQ
                     ADVHVGDFEKPGVSLARALAGIDKIFLVTDGPDLDLRDRAVAVAAKSAGVRHVVKLST
                     LDVHTGVGTGTWHARGELAIRESGVAFTFIQSAGFMLNALGWAHSIRERRVLRTPTGQ
                     GKIAFIHEDDIADVAIAALTTRDYHDQSLIITGPEALSYGEMAAIIGGAIGKRVRFEQ
                     ISDQQAYAGVVRWAGKGLYADAMIDIWRAVREGRLANVSDGVQRVLARKPISFHRWVE
                     ENANAFQ"
     gene            complement(11807..12373)
                     /locus_tag="VFQ35_23990"
     CDS             complement(11807..12373)
                     /locus_tag="VFQ35_23990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9933795.1"
                     /translation="MEAVIEAVPRVLRRHGVDAITTNRVAEAAGVSIGSLYQYFPDKQ
                     AIFVALHDRHVDGVRHVIERTMTDCASASLEVFTGELVERLAHVHSEDAELHEIISVA
                     VPGSAVGFKSALQATFEQVISPTKQDRYTPEAAERMLFVLPQMVEALVHGVAQQRQAS
                     SRERAKDEAIRTVAVYLNSYQGGVPNRY"
     gene            13135..14031
                     /locus_tag="VFQ35_23995"
     CDS             13135..14031
                     /locus_tag="VFQ35_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9933796.1"
                     /translation="MHIFVTGASGFVGSAVVRELLGAGHTVLGLAHSDEGALRVKALG
                     ADVHRGAIEDLASLERGARASDAVIHTAFNHHDYSVFAQSCENDARAIEAIGGALAGT
                     PHPFIVTSAVGVLQKGGVADENTALSQHPRAATERAVNAVAARGVRVSIVRLPVSVHG
                     DGDPNFVTALIRIARQKRASVYLEDGSNRWPAVHVLDAARAFRLAVARPLPSGTRLHA
                     VAEQGIAFLDLATAIGRRLNVPVVSKPRSEGVDHFGGFLANFAGMDIAASSAHTRDAL
                     GWAPDHAGLIADLEQSHHYFAS"
     gene            14181..14897
                     /locus_tag="VFQ35_24000"
     CDS             14181..14897
                     /locus_tag="VFQ35_24000"
                     /inference="COORDINATES: protein motif:HMM:NF036522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9933797.1"
                     /translation="MASRRTPQISTRKEPQQARSNDLVAAALRAAIRVLAKEGAQRFT
                     MARVAEKAGVSVGSLYQYFPNKAAILFRLQSDEWRQTAKLLRDILEDARHPPLARLRS
                     LVHAFIQSECDEAAVRVALNDAAPLYRDAPEAREIRASSEATLEAFMREALPEAADGM
                     RTSAGDMVMDTLSAVGKQFSESPRTPAEIRGYADALADMFCAYLAFLSSAPDKGGGAE
                     RSNRSARSPRRRSGQSLAGR"
BASE COUNT         2656 a         4892 c         4766 g         2667 t
ORIGIN      
        1 gcggccgtga aaaacttcgc gacgtacagc ttgaagaggc tcggtaccga ggtcatcgac
       61 gtataccgac cggcacggct cgaccccaac gtccccgtag aggacacggt cggggcaatc
      121 gcggagctga tcgaggcggg ttacgtgaag tacgtgggcc tctccgaggt cggagcgcag
      181 acgattcgac gcgcgcacgc ggtgcacccg gtcgccgacg tgcagatgga gtactcaatc
      241 gcgacgcgca cgccagaaga gaaggtgttc ccggtacttc acgagctcgg catcggcgcg
      301 acgctcttcg gcgtgctcgc acgcgggctg ttgagcggaa gcaagccgag cagcccgaaa
      361 gacgtacgtg cccgcctgcc gaggttctct ggcgacgcag ccaaggagaa cgaagtcctc
      421 gtggagaggc tccgcctttt tgcagccaag gccggccgca ctccggccca ggtctgcatg
      481 ggttgggcgc tcgcaaaaga gccggcgttc gtaccagtga gcggcgcgca caaaccggca
      541 caactcgacg ttctcgatgc gctcaatctg cccctctcgg cggatgagat ggccgagctc
      601 gagaacctct taccaaaggg ctctttcaag ggcagtcgct accccgaagc tcagatggct
      661 gacctcgaca gcgagcgttg actcgactta gtcgagccat tcgcacgcgc ctagcctccg
      721 taaggacggg tgatgatctc gaggaagtgc ccggccgggt catggaaata gatgccgcgt
      781 ccgccgccgt catgattgat ctgctgcgga agccctgccc ccggatccgc ccagtagggc
      841 acaccgcgcg cctcgatgcg cgcgaagatt tcgtcgaact cggcttcact gatcaaaaac
      901 gcgtaatgcg tactctggat ctctccctcc acctcgagga agtcgagcga cactccgttc
      961 gccgtgttga cgacccaaaa gtgatagaac cgccgcggcg caggcagccc cagaatgtcg
     1021 gagaggaact cggcgcttgc cttggcgtca cgcgaaggca cgatggtgtg gttgagctgg
     1081 actgtcaccc gagagacgtg gcgccgcgtc cgaaggcgcg caacccctac gaggcccgat
     1141 cgcgcgcgaa cgcgcggcat ttgatggatt cgaaagcctc ggctattctt tgcgcgcaat
     1201 gaagttctcc gtgtttctgc tcggctgagc gtcggttagc cgacgcttct cccttgttgg
     1261 cgcgacgcgc tcgtcgtcgc ctcgcgtgct tgtgcctcga gctcgaatag ccggagatct
     1321 taaaatgcct catcgtgatg tcgcgctttc caccgcgcag gtcgaacagt tcatctacga
     1381 cggcttcgtc cgcgtcgacg aggccttccc gcgcgaggtc gccgacgcct gtcgtcgcat
     1441 cttgtggctc gcgactgggt gcagcgagga cgcgcccgcg acgtggacgc gcccggtcgt
     1501 acgaatcggc gagatcccgc accccctctt ccgcgaagca gcgaacacac cgcgcttaca
     1561 cgcggcctac gacgcactcg ttggaccggg ccgctggctc cctcgaggaa gtctcgggac
     1621 gtttcccatc cgctttccct cggctgaaga tccgggcgac tgcggctggc acgtcgacac
     1681 gagcttcggc tcggaaggag agcccgattt cctgaaatgg cgtgtgaacg tggcctcgaa
     1741 ggggcgagcg ctcctcatgc tcttcctctt ctccgacgtt ggtgacgacg acgcaccgac
     1801 gcgccttcgc gttggttcgc accgcgacat cgcgcagagg ctagaaccgc acggcgagca
     1861 ggggctcagc ctcggagagc tcgccagctc ggggttcacc gagtcagcgc atcgacccga
     1921 agccacggcg acggggccgg cgggcacggt gtacgtctgc cacccctttc tcgtgcatgc
     1981 ggcacaacga ctccacgttg gagcgatgcc tcggttcctt gggcaacctg cactgctgcc
     2041 caaagtgccg ctcgatccct ggcgagacga cgcctttcca gtcgagcgag ccattcggct
     2101 cgccctcgac gctcgctgag cgtgcggctc cgggagcgaa ctttgttgtc gctcgtggag
     2161 cgcccccgaa ggacttagtt tctctggtaa gacctatgag gtctcgaagg agcgtaaaca
     2221 atgtcttgga cgcaacggtt cgcgccgctc atgctcgagc gatggaaaag cacaggcgtt
     2281 cgtggtggcg atgagagcgc tcgtggtcgg cggaaccggc ctcgtcagca ccggcgtcgt
     2341 gcagcagctt ctcgctcgcg gcgttcgagt ttcggtatcc aatcgcggaa cacgctcgaa
     2401 ttcgttcggc agtgacgtgc ggctacttcg cggcgaccga gccaatgctt ccgagttcgt
     2461 gcggaccttc gagcgcgagc agttcgacgt cgtctacgac atgatctgtt attcgcccga
     2521 ggacgcggag gtcagcgcgc gcgctttcgc cgggcgctgt gaacagttgg tcctttgctc
     2581 ctccgcgatc gtctatggca ggcggatgcc gtcgagtgtg ttgatcgacg agagcgcccc
     2641 actcgaaccg acgagtcact gggggaagag caaggtgctc tgtgagcagg cgctccagcg
     2701 tgcggccgac aagggcgcct tcaagctcac gatcgcgcgg ctcggtcaca cctatggccc
     2761 cggcgatttc atgaacgacc aacaggacaa cgacggcacc gcttgggacc gcgtggttcg
     2821 gggtctaccc gtcttctgca cgggggacgg gctcggcttg tggcagtcga cccaccgcga
     2881 cgacaccgcc aagttctttg cctttgcggc gctggcaccg cagacgtacg gcgaggcgtt
     2941 caacgtaatg cgcgacgaga tcctcacgtg gcgtgactac tatcgcgagg tcgctcgttc
     3001 gctcgatacc acggcaaact tgctcttcgt ctccgcgtcc tggctgatag cgcaggcgcc
     3061 ggagcgcttc agctttttgg cggagatatc ccaatttcac gccgcgtact cctcagcgaa
     3121 agccaaagcc gcggttcccg agttccgcgc gacgatcggg ctcgaggccg gcgctcgcga
     3181 gacctttgct gacacgcggc ggcggggcgt ttgggctgat agctcccgtg acaccagcta
     3241 cgacgagctg gtggaccgcg cgttgacgct gggctttcgc gtcgaagcag cgtgaaggca
     3301 ccgcgggtcg agcgtgcgct cgggtgcgaa tgacgcccgc gtgacgaaat cgctccactg
     3361 ccccgttcca tccgctcgct cgtccccaga agcgctccat cgcctcgttt cgagcgaggc
     3421 gggcgcgtca ggagggagca ccggacgcct accaccgtcg taagaattca tcccgatgcg
     3481 tccccgtgga acaagccgac gacgtcagcg cagctctgcg ctcacgaggc gaacgtcctt
     3541 gaacaagcgc gacgtttcgc cgccgaggcg tgacgcgatg tgcgccaagt cgtcatcgtg
     3601 aaggaccgag aacccgtggc tctcgagcag agtacgcatc tcatccgcac tgaacgccga
     3661 acgaaagggc tctccgatga gccctagcaa aagcccggcc gtcttccgaa cgattgagtg
     3721 agacgtgtac aaaacgatga gccggctgcc tggggcggag cgcgcccgca cgactcggag
     3781 cgttgcctcg atgtcctcgc gcgcgaggta catgaccact ccttcccaga gccacgtcgt
     3841 cacccgcgcc gggtcgtgac cggccgcgac gagcgcgtct tcgaggcgat cgcgcgtgaa
     3901 gtccaccggg gcgaagtgaa gctctcgcgc tgcaagcttg agcgaggccg cgcgctcgcg
     3961 cttgcgccgc tgcgagtcgg ggtggtcgac ctcgaacacc gtcgtatcgg cgagctctgc
     4021 cattcgccac gcgcggccgt cgagaccagc gccaagaatg acgagttgcg gtgacgcggc
     4081 ctctcgaatt gccctgtcga tggcgacagt gcgaggcacc atcatcttga ctcgcccttc
     4141 caccatacgg cgctgcatgc gctcgctaag gccgctcggc ggcacgccag caagctgtcg
     4201 ttcgatgcgc tgccgagcat ccgccgggag caaggtgagc gcagtcgggt ccgcgaacgc
     4261 accaacggcg gttcgaccgt gggcggcagc tcgggccgcc gcgaccatga ctgcagtttg
     4321 gctctctgtt cctggcttca tgtgaaactc ctcatcgctt tgctcgcctc cgccgtgcgg
     4381 gttcgggcag cccacgctcg atcagctcca atgcttcacg aatcatcgga atgagcgggg
     4441 gcggcctctt cgaccgcagc catctctcca agacggtcgt gacggcggca acggccgtcg
     4501 cggcgactat cgaagggtac atgtcgcgct ctgaatcgag cccggtgcgc tcggcgacgg
     4561 cgagggcgag cgcccgctga gcggcgagct ctcccttcca gagctcggcg cgtagcgtcg
     4621 cgtcctccaa caccaagagc gtagcctcgc gactctcaga ggagtcgttg agaaccagat
     4681 tctcgtacgg cgcgaccatc gccgcgctga gcgcctgcca cagcgtctcc gcacgcggcc
     4741 gcgcgcgcag tactttggcc gagtgctggg cgcgctgcac gagacggtcg gcgagcgcct
     4801 cgtacttgct cgcaaagtag ttgctgaagg tgcgccggga cacccctacc gccgccacga
     4861 tgtcctcggt ccgtacgttc tcgatccccc gctccttggc cagccgtagc gcggccaggg
     4921 tcagctctcc gcgcagcgct cgcttctttc gttcgcgaag gccgagagcg ggcgccgatg
     4981 gctcgggacg ggttgtgcgg cccatgccca ttgcctagcg ccaaagtgct caatgcgcaa
     5041 gtttactcag taagcaaact tgctcactag gcaagtttat accccgacgg cccgcgcccc
     5101 tccgaaatcg aggccccccg ggaccctcga aaccaggggg gcatgaactc gtgagcaacg
     5161 ggctctactt cttcgcgctg cgtgccttct gcggcttcgc cgtggctcgc gtggccgcct
     5221 ttgccgggcg tggcgggtgg agcgtttcgt gccgtgcgca catttcgacg aagcgctcga
     5281 ttcgggcggc tcgcgtctcg ggcttcttcg cggtttgcac acggtagagg atcgcgtacc
     5341 ggttcgcgcc gtcgagggtc tcgaaaaagg ctcgcgcccg cgcactccgc gcgaacgccg
     5401 cggcgaggtc ggcgggtatg ctcgcagcgc gcgctccatc gtatgcgctt tcccatcgtc
     5461 catcacgctt ggctcgctcg acctcggcga ggcccggagc ttccatcgcg cctgcggcga
     5521 tcagcgcttc cgccttggcg cgattgatct tcgaccaagg gcttttcgcc gtacgcggac
     5581 cgaaccgttg aagccacgct tccgagtcga gcgcgcgctt ctggctgtcg atccatcccc
     5641 acgcgagcgc cacgtcgaga gcttctgcgt aggtgatcgc attctttacg ccctttttcg
     5701 ggatgcgcag gaatacgcac ggcgagtgag agtgattgga ccgaagccac tccgaccact
     5761 ctccgacatg cgagaactcg agggtcgatt ccgctctctt cggttcagcc tttttcatcc
     5821 gtaactcttg ccgtcgaacg tctgtacggg gaccatcaac cagcccgtcc ggctacagat
     5881 ggagcaattg catgcgctcg cgcgagagct ctgcgtagtc acctcgaagc gaatcttccc
     5941 gcagtggcat gatccagaat aggtcttcgt ttccgacatc ttcctcgtcc acgggctgat
     6001 accccccaag ctccctcgcg tattgcaaaa gtgcgccaca tcgggagaat gtcggggtgg
     6061 gaacgaaggc cacggtcgga attctaaacc ccttcgaggg cttcaagcac ttttcgctcc
     6121 gacgcgaggc tcctcccgag gatctagctc cgttcgtcga gcgcttttgg agcgtgcgct
     6181 gggacctcga tgggagaccg ccgttcgcgc aagaaatcct gccgtacccc tgcgtgaact
     6241 tgtcgactgg aagcggtggg ttcgaggtgc acggcccggc gacgaagcgc ttcgtcgctc
     6301 gactctctgg ccgcggcgag gtcttcggga cgaagttcac gccagccgga ttcttagtat
     6361 tcactcggac tcccctgcgt gacctcgtgg atcgcatcgt ctcgatcgaa gctgcggctg
     6421 gacgaggccc gtgcgcaccg acgactcacg atttcgatgc cgtacggccc caagtagaaa
     6481 cgttcctccg aagctttcac cccaagccca cggaggcgat ggcgctcgcc gatcgactgg
     6541 ttcggctcgc gcaagaagac cgatccatca cgcgcgcaga ggatcttgcg cgcaccgccg
     6601 gcatctccgt gcgctcgttg catcggctgt tcgaacacca cgtcggtgtc gggcccaagt
     6661 gggtcattcg gaggtcacgg gtacaagagg ccgcggagcg cgtcgcgcgc ggcgagccgg
     6721 tcgactgggc aggtgctgcg caggagctcg gctaccatga tcaggctcac ctgattcgcg
     6781 actttcgcga gcaggtcggc gcgacccccg ctgcctacgc gcggcaatgc ggggcctcgg
     6841 tcgtcggctg aagttccggg ccggggccga cgcccacgcg cttcgcccgt acgcttcacg
     6901 actgcgcgcc agccgaaggc acgctgtcga tggctcatga gagctcagac ttcagggctg
     6961 ctaccgccgc gtgcaggccg gcgccgcgat ctggaccagc ctcccagatc atcctcaact
     7021 cggcttcccg agtgaccacc tcctccacca acatgcgcgc cgcctcgacg gctcgatcca
     7081 gaagcccctt ctcgggcgtt tcgggtaggc ggccatgcag tgccgagggc caatcgtcgc
     7141 acgagcgcac tttgcaccag attgcgacga tcccaacgaa cgcgtaggag cgacgttcgg
     7201 ggtactcgtt ggggcggccg tatccgatca cgccgcgcgc ttcgtcgtga aatccgcgga
     7261 ttgcattctc taccccgtag tcctctatcc atttcaggag tccctcgacg atctcgacgt
     7321 caccgaaggg tccaggatcg gtgacgtcac gacgagacgc ccacttcgtc gcgtttcggg
     7381 cggtcaattg ggcctcgcgc tcgtcgggag accggacacg ctggatggcc ttctttccga
     7441 gcgcaatggc gcggatagcg tcctcgccat acgggcggag cagctcacgg tcgcacgcct
     7501 cctcgtaaga cacgacgcgc gcgccacctg cgctggcagt tgccaagatg tacccggtga
     7561 tcgcgcctat ctcgccggcg tagagcttct tgcccctgag atcctttcgc accggtacgt
     7621 tgccgagaac acgacctttc cgcaagagct cgttcaccgg tgccggaagc cgctgcgccg
     7681 ggccgtgctt cacggtcgcg agcgccgtag cgtcgtcgac gaacgtgccc atcattacgg
     7741 caacttcacg aggagcgacc acctggacga cgatcatgtc tcccgatgac gaagcagccc
     7801 cgagtgcgat cacgtctccc ttcttcgctt tcggcgactc gcgcgccccg ctgctcgccg
     7861 cctcgagccg cgtccgcagg tgcgcaatcg aagcatcgaa cccggcgcca tcgctgccct
     7921 cgtgccacaa accagcgagc tccgacgtcg tgcggtcagc aatgcgcgtc agcacttcga
     7981 gcgcaagtcg gcgttgttcc tgcttcggag tgagcctcgc ggccatgtcg agcaccgcgt
     8041 cacctgctgc gtccgcatca ccgtagccga aagcaagcgc cacgagctcg caggccgccc
     8101 acgctgcccc gccgtcatcg acatcaatgt aattgccagc cggtctcttc gcgacgtcac
     8161 gcaaagcctt ggccacgaca cgcgaaggtg atgcgtgcag ctgctcgagg aagtcggagg
     8221 cagcatcgtt gtcgaacgga ccgggacccc acgtgcccat cgctctttac cgctgttcga
     8281 ggaattgcgc gaagcgcttc gcgaggatcc aagccagact cgatcctccg gccacttggt
     8341 aacagctccc gaaccgttcc tcaaacagga actggtcacg cgaccgtacc cgaagcgtgt
     8401 gctccggccg gaacgaggga ggcggcggct catcacgatg cctcgtcttg catgatgcga
     8461 tgcacctcgc tataatgaac cttatggttc agtattcgag tgaccgcctc gacgcctcgt
     8521 tcgccgcgct ctcggatgcg acccggcgtg gcgttttgga ccagctcggg cgtgcagacg
     8581 cttcgatctc ggacctcgcc gagaagttca aaatgaccct gactggcatg aagaagcacg
     8641 tcggggtcct ggagcaggcg ggcctcgtct cgaccgagaa aatcgggcgt gtgcgaacct
     8701 gcaggctcgg tcctcgtcgc ttggaggagg aggcagcgtg gatcgagcgc taccgacagc
     8761 tctgggccgc gcgcttcgac gagttggaca cggtcgtgga tgaactgaaa cagaaggaga
     8821 agagcaatgc ccgaaagaaa cgatagtgct ggaaccacca cgaagaatcg cacgaccacc
     8881 gagcggaagt cggagcgcga gctcgtcgtc acacgtaccg tcaacggccc ggcgcgcctc
     8941 gtgttcgaag cctggaccaa gccagaactg tttcagcgct ggtgggtgcc gaagtcgttt
     9001 ggtctgacgc tgctgtcgtg tgagatggat gttcgcgtcg gaggtcagta ccgcctggtg
     9061 ttcaagcacg aagattcgac gatggcgttc ttcggtacgt accgcgaagt aacgccgccc
     9121 tcgcgcctcg tttggacgaa cgaggaagga gaagatggcg agacggtgac cacggtcacg
     9181 ttcgacgaaa aggacggcaa gacattggtc gtcgtgcatg acctatacgc ctcgaaggag
     9241 gcactcgacg ccgcgatcgc ctctggatcc acgggcggac tgccagagca gctcgacgag
     9301 ctcgaggcac tgctcgaaag cttcggcgcg accccggaga acggatgacg acgcggtctc
     9361 cgcttcgcgc gaagcgcgcg gccgccccca aaagcaagaa ggctcccgcg gccaagcccc
     9421 atgcgaaagc cgcggccaag ccccgtgcga aagccgcggc caagccccat gcgaaagccg
     9481 cggccaagcc cgccgcaaag cttttggttg cgatgactgg caaggcgagc cccgcgaagg
     9541 cgaaggaagg cgacgagccc gtgttcgcgt acatcgccag cttgccgcag ccccagcgtc
     9601 gcatcgccga gcgcgtcgat gctctggccg ccaagacgct gcctaacctg cagcgtgcgg
     9661 tgaagtgggg catggcgtac tacggtgtcg acgggggctg gtgtttctca tccggcgcat
     9721 tcatcggcca cctgaagctg atgttcatac gcggcaccga gctcgaacca gagccacccg
     9781 tcacacccat cggcatgggc aagtccacgc gcggggtcga gctggcagcc gtgaacgagt
     9841 tcgacgaacg ccagttggcc gcgtggatga agcaggccgc gaccatgccc ttcgttggcg
     9901 gaaagaagaa gcggtgaccg ctgcacgcgt ggatgacgcg agagcggcga cggcactcct
     9961 cgcgaagctc ttgggcgagc ggcaggccgc gatcgagcga ggaatctgag taagcagcaa
    10021 gaggccatcc aagtccggag tggtttcagc ggtttaggga atgggcaacg cgagctgacg
    10081 tacccgacgc cgttcgttag tttcctctcg aacagtgagg agctcgaacc gatgtcgaat
    10141 gaacagatgc gttggctttt gaaggtcatc caacccgcgc ggacgctggc ttccctgccc
    10201 ggaggtgctg ggctgtcgca ggaagcccat gcagcgctgc tcggacttcc gccggagggc
    10261 tacgccgctg agctcggccg catgaaagcg gaggccaagg aagcggcgag cgagctgctg
    10321 gccgaccccg ccatcgcttc gatggtcgac cgacttccgc tccggaaagg cgcgacggtc
    10381 gctgcgttcg gagacagcct gacgtcggat ccgcagtctt gggctgtaat cttgagcgag
    10441 ctgctgagcg cgcgccgtgc cgccgacggg atatcagtgg ccatcagtgc tgtcggcggc
    10501 gacacgacta cgcacgggct ggtccgggtc ggcgaagtcg ttgcgcaacg tcccgattgg
    10561 atcttcttct tcatcggaac gaacgacgca cgaacacagg gttcccaccc gaccaagacg
    10621 ctcgtccacc atgaggagac catgcgcaac atcgcggagc tgcgccaacg cgtagcgcgc
    10681 gaaaccaagg cgcgcgccgt gtggatcacg ccgcccgcgg tcaacgagga gcaggtcgcg
    10741 agtcattggg gactctcccg cttcggagtt cgcttctgca acgaagatct ggcgcgcgtc
    10801 gccaagatcg tccgcgacgt ggatggcgcg gcgatcgatg tcttttccat gctgggaacg
    10861 ccaccctctg ccgacctcct catgggagac ggcttgcatt tcacggttgc cgggcagaaa
    10921 cgcttggcgc tcgaggtgct caagggttgg agcaacctcg aatgaagtat ctgatcttcg
    10981 gcgcgaccgg gaacatcgga tctcgagtca cccagcgcct catcgctcgc ggcgaacgag
    11041 cgtccgtttt tgtgcgcaac gcgaagaagg caaaggcgct cttcggcgag caggcggacg
    11101 tccatgtggg tgacttcgag aagccggggg tgtcgctggc gcgcgctctc gctggtatcg
    11161 acaagatctt cctcgtcacc gatggtccgg acctcgattt gcgagatcgc gcggtcgcgg
    11221 tcgctgcgaa atcagcaggc gtccggcatg tggtgaagct ctccacgctc gacgtgcaca
    11281 ccggcgtggg caccggaacc tggcacgcgc gcggcgagct cgcgatccgt gagagcgggg
    11341 tggcgttcac gttcattcag tcagcaggct tcatgttgaa cgcgcttggt tgggcgcact
    11401 cgatccgcga gcggcgcgtg ctgcggaccc cgaccggaca gggaaagatt gcgttcatcc
    11461 acgaggacga tatcgccgac gtcgctatcg cggcgctgac cacgcgcgac taccatgacc
    11521 agtcgctgat catcaccggt cccgaggcac tgagctacgg tgagatggct gcgatcatcg
    11581 gcggcgcgat aggcaagcgc gtccgcttcg agcagatctc ggaccagcag gcgtacgcgg
    11641 gcgtggtccg ctgggcgggt aagggcctct acgcggacgc gatgatcgac atttggcggg
    11701 cggtgcgcga gggccggttg gccaacgtct ctgatggcgt gcagcgggtc ctcgcacgaa
    11761 agcccatctc attccaccgt tgggtcgagg agaacgcgaa cgcctttcag tagcggttcg
    11821 gaacgccgcc ctgatacgaa ttcaagtaaa ccgcgacggt tcgaatggcc tcgtctttgg
    11881 cgcgttcgcg cgagctcgcc tgcctctgtt gagccactcc gtgcaccaaa gcttcgacca
    11941 tctgcggcag gacgaagagc atccgctccg ccgcttcggg ggtatagcga tcttgcttgg
    12001 tgggcgaaat cacctgctcg aacgtggcct gcagcgcgct cttgaagccg accgcgctcc
    12061 ccggcaccgc gacggatatg atctcgtgca gctcggcgtc ttccgagtgc acgtgggcga
    12121 gccgctccac tagctcgcca gtgaacacct cgagtgaagc cgaagcgcag tcggtcatcg
    12181 tccgctcgat cacgtgccgc actccgtcga cgtggcgatc atgcaaagct acgaagatgg
    12241 cctgcttgtc gggaaagtac tgatagagcg agccgatact gacgcccgct gcttccgcga
    12301 cgcggttcgt ggtgatggcg tcgacaccgt gacgcctcag cacacgcggc actgcctcga
    12361 tgacagcctc caccgtctgt cgggagcgtt gctgcttggg ttgacggcgt gtgggcgctc
    12421 tttcgctgaa cttcatagga attcgtgctg accgcagatg actcaagcta gcactcacct
    12481 agcctcgcgg tacacgaaca gccccggctc gccacgcccc cagggtccgt ccaatcggcg
    12541 atggctcccg ccatgagagg ccatcttgtc gcaacggagg cttaccacga ccgtcggcaa
    12601 tgtcaggcgg acttaccgag cttttcagtt ggaagggcgg cttcgtcgcg acgaactgtg
    12661 cgtggagcgc gtgaacctcg gccgcggtat cgacctcgag tccgaagtgg aatgcgaacg
    12721 gccaactcat cgggcggtcc gagctttcga tgacgaggtc gaactcgccc cgcttcagca
    12781 cggcccattc ggcccttggc tcacagcgcc agagaatgcg actaaagcgt ctggttcgtt
    12841 aatagcgttg gctgagctcg gtcactcgac gagcgcaaca ggccgaacgt tccaaacctg
    12901 aatagctcga gcccgtgacc ctcctcctgg gcgattcggc gcgcctcgtc tgctcttcaa
    12961 cacgcggaac tccccggctt cgcactcgga cggagcgctc tctctccgcg cgcgcggaat
    13021 gcgactagcg aacctgaaca attcatcgca ttctaactac gaggcccgcg gagcaattac
    13081 caagcgctgc aaaagccgcg tcaatcgacg tcggctagca gaggagaaat cagtatgcac
    13141 attttcgtca ccggagcttc agggttcgtt ggttctgcgg tcgtccgcga gttgctcggg
    13201 gcgggacaca cggtgctcgg cctcgcgcac tccgacgagg gtgcgctgcg cgtcaaagcg
    13261 ctcggcgccg acgttcatcg tggcgcgatt gaggatctcg cgagcctcga acgcggagcc
    13321 cgtgcgtcgg acgccgtgat tcatacggca ttcaaccatc acgactactc ggtcttcgcg
    13381 cagagctgcg agaacgatgc acgcgccatc gaggcgatcg gcggggcact cgcgggcacg
    13441 ccacatccct tcatcgtgac gtcggccgtc ggggttctcc aaaagggcgg ggtcgccgac
    13501 gagaacacgg cgctttccca gcacccgcga gcggcgaccg agcgcgcggt gaacgccgtc
    13561 gcggcgcgcg gcgttcgagt gtcgatcgtg cgcctgcccg tgtcggtgca tggcgatggg
    13621 gacccgaact tcgtcacggc gctgatccgc atcgcccggc agaagcgagc ctcggtctac
    13681 ctcgaggacg ggagcaaccg ctggcccgcg gtgcacgtcc tcgacgcggc tcgcgccttt
    13741 cgactcgcgg ttgctcgtcc gctccctagc ggcacccggc tacatgcggt cgcagagcag
    13801 ggcatcgcct ttcttgatct cgccacggcc atcggccgcc ggctcaacgt gcccgtcgtg
    13861 agcaagcccc gtagtgaggg cgtggaccac ttcggaggct tcttggccaa cttcgccggt
    13921 atggacatcg ctgcttcgag cgcgcacacg cgcgatgccc tcggctgggc gccggatcat
    13981 gcgggactga tcgctgacct cgagcagagc caccactact tcgcgagctg acgtgaatcg
    14041 agaggcaggc ctaacagggc gttgaaaaac gccctgctag aatgctgacc ggcatcaccg
    14101 cgcggctgcg cagtactcgc cccccggcct ggacacaatg cgatgaatcc gtcagatttt
    14161 gacctgtgac cagagcgcgc atggcttctc gacgaacgcc gcagatatcg acgagaaagg
    14221 aaccccaaca ggcgcgctcg aatgacctcg tcgcggcggc cctcagggcc gccattcggg
    14281 tcttggcgaa ggagggagcc cagcgtttta cgatggctcg cgtggccgag aaggcaggcg
    14341 tcagcgtcgg gtcgctctac caatacttcc cgaacaaggc ggccattctc ttccggctcc
    14401 aaagcgacga gtggcgccaa acggctaagc ttcttcgcga catcctcgaa gatgccaggc
    14461 accctccgct cgctcgcctc cgctcgttgg ttcacgcttt catccagtcc gagtgcgacg
    14521 aggcggccgt gcgcgtggcg ctcaacgacg ccgctccgct ctaccgagat gctcccgaag
    14581 cgcgtgagat ccgcgcgtcg agcgaagcca cgctcgaggc cttcatgcgg gaagcgctac
    14641 cagaagcggc ggatggcatg cgtacgagcg ccggtgacat ggtgatggac acgctcagcg
    14701 cggtagggaa gcagttctcg gagagcccgc gaacacctgc cgagattcgc ggctacgcag
    14761 atgctctggc agacatgttt tgcgcctacc tcgcgtttct ttcgagcgca ccggataagg
    14821 gcgggggcgc cgagcgcagc aaccgaagcg cgcgctcgcc tcggcgtcgc tcggggcaat
    14881 cattagcagg gcgttgaaaa acgcctgcta gaatgctgac cggcaccacc gcgcgtcgtg
    14941 tgtgcctggc attgatctgt cgtgacgcag ggcgtttttc a
//
LOCUS       DASTKS010001132         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_625747, whole genome shotgun sequence.
ACCESSION   DASTKS010001132 DASTKS010000000
VERSION     DASTKS010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625747"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFQ35_24005"
     CDS             <1..468
                     /locus_tag="VFQ35_24005"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9933798.1"
                     /translation="EQTFTQEDQALAAAFVGNFGAILRALDVVSFLILGIVLLVLGNT
                     IAMSVRERTREYGVLRAIGFLPSTIVGFIIAEAALLGLAGGALGVLLAYPLVERPVSR
                     YFEESMHFAPLQVPTSAAVVTVAVGALLGALAAAAPALRASRLEVVGALRRIG"
     gene            477..1643
                     /locus_tag="VFQ35_24010"
     CDS             477..1643
                     /locus_tag="VFQ35_24010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9933799.1"
                     /translation="MALPFRYSFRNLWVRRTTTFATIVGIGLVVFVLAATLMLAAGVK
                     RTMTSAGRADRAIVMQGDAYSEGDSRMRQSVLGIVGAAPGVRRDAAGEPLVVGETIVH
                     VYLSAADDPSSIDSVQIRGISAKAFLVRDEVRLVEGRLPKPGTDEAIVGKRLFGRYQG
                     LRLGDGFELQKKRRVTIVGAFAADGAAYESEVWADLDAVRSSFGFQGYLSSITARLES
                     PQSFEGFAGTLRADKRQGLSVERERAYYEKVSEGLSHLITGLGSVVALIFSLGALLGA
                     AITMYGAVSQRTREIGVLKALGFARTEVMLVILLESTLLAAAGGALGIGVALLTPLFD
                     FSAMNYATGQQLVFRFVPELGVLGRALASGTLVGVLGGLFPALRAASVNPTELF"
     gene            1698..2354
                     /locus_tag="VFQ35_24015"
     CDS             1698..2354
                     /locus_tag="VFQ35_24015"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9933800.1"
                     /translation="MLRLVLGNLNYSSWSMRPWLALKRAGAKFQVHDVGLRTAPDWKE
                     RILSFSGAGKVPILVDGSLSIHESLAICEYVAELHPEAKLWPADMKLRARGRAISCEM
                     LSGFSALRSCMSTNLRARAEKTPDAPGVRADIARIFDIWTASLESTGGPFLLGEFSIA
                     DCMYFPVATRFRTYGVGLPERLARYSAALFGTPEARELEQIAATTPAIPEYDVALRPL
                     "
     gene            complement(2411..2833)
                     /locus_tag="VFQ35_24020"
     CDS             complement(2411..2833)
                     /locus_tag="VFQ35_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9933801.1"
                     /translation="MKTRKIAYFTTTALVSFAFVAGGAADVTSAPAVIDAMHKLGYPA
                     YVASLLGVWKLLGAAALLAPRLPRLKEWAYAGIFFDLTGAAVSHAAVGDAAGKIMTPL
                     VILGLAIASWALRPADRLLASRPVTRADAPSGRAVLAA"
     gene            2935..3924
                     /locus_tag="VFQ35_24025"
     CDS             2935..3924
                     /locus_tag="VFQ35_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9933802.1"
                     /translation="MQDPIETSELLAFVKTVETHSLSRAAAELGVPRATISRRLARLE
                     ERLGARLLRRTTRSLVLTDAGEMLHRHARIVLDAVAQAEGSVRQADPVVRGALRVSAP
                     SLINEAFVDMVADFVRSHPAVQLHLHISSQHVDLRRGGHDVALRASSELEPGLVARTL
                     MRTTLIAVAGPSYLKQYGAPKNGDDLANHRCLVGFARGELPQTHWPLVRGGKLPVEGA
                     FFTNELPVLLDFALRGLGIALIPVNVAQPYLDSGELVHVLSGEVGAESRFSIVYPERE
                     FVPPQLRAFIDAVVAWRRVGEAISPPRVGGGSAKRKKRNVKNPKPQPRRGTGS"
BASE COUNT          637 a         1293 c         1290 g          709 t
ORIGIN      
        1 gagcagacct tcacgcagga agaccaggcg ctcgccgcgg cgttcgtggg caacttcggc
       61 gccatcctcc gtgcgctcga cgtggtcagc ttcttgatcc tcggtatcgt gctgctcgtg
      121 ctcggcaaca ccatcgccat gagcgtacgg gagcgcacac gtgagtacgg ggtactgcgc
      181 gccatcggct ttttgccctc gaccatcgtg gggttcatca tcgcggaagc ggcgctgctc
      241 gggctcgcgg gaggagcgct cggggtgctg ctcgcgtatc cgctcgtcga acgccccgtg
      301 agccggtact tcgaagagag catgcatttt gctcccctcc aggtgcccac gagcgcggcc
      361 gtcgtcacgg tcgcggtcgg cgcgttgctc ggagcgctcg ctgccgccgc gccggcgttg
      421 cgggcgagcc gtctggaggt ggtcggcgcg ctgcgccgca tcggttgagg agacgcatgg
      481 cactgccttt tcgttacagc ttccgcaacc tgtgggtgcg gcgcaccacg accttcgcga
      541 cgatcgtcgg catcgggctc gtcgtgttcg tccttgccgc gactctgatg ctcgcggcgg
      601 gtgtgaagcg cacgatgacg agcgcaggga gagcggaccg cgccatcgtc atgcagggcg
      661 acgcctactc cgagggcgac tctcgcatgc ggcagtccgt actcggcatc gtgggtgcgg
      721 cgcccggagt gcggcgcgac gccgcgggtg agccgctggt cgtcggcgag accatcgtgc
      781 acgtgtacct tagcgcggcc gacgacccga gctcgatcga cagcgttcag atccgcggca
      841 tcagcgcgaa agctttcctg gttcgcgacg aggtgcgcct cgtggagggg cgcttgccaa
      901 agcccgggac cgacgaagcc atcgtcggta aacgcctgtt cggtcgctat caaggccttc
      961 gcctgggcga cggcttcgag ctccaaaaga agcgcagagt caccatcgtc ggcgcgttcg
     1021 cagcggatgg cgctgcttac gagtcggaag tctgggccga cctcgacgcc gtgcgttcgt
     1081 cgttcggctt tcaaggctac ctgtcgtcga tcaccgcgcg gctcgagtcg ccgcaatcct
     1141 tcgaagggtt tgccggcacg ctccgcgcgg acaagcgaca agggctgagc gtcgaacgcg
     1201 agcgcgcgta ctacgagaaa gtctcggagg ggctcagcca cctcatcacg gggcttggta
     1261 gcgtcgtggc gctcatcttc tcgctcggtg cgcttctcgg cgccgccatc acgatgtacg
     1321 gcgcagtcag ccagcgaaca cgcgaaatag gcgtcttgaa agccctcggc ttcgcgcgca
     1381 ccgaagtcat gctggtcatc ttgctcgagt ccaccttgct cgccgccgcc ggcggtgcac
     1441 tcgggattgg ggtcgcgctg ctgacccccc tgttcgactt ctccgcgatg aactacgcga
     1501 ccgggcagca gctcgtgttt cgcttcgtgc cggagctcgg cgtgttgggg cgagccctcg
     1561 cgagcggcac cttggtcggc gtcctcggcg gattgtttcc ggcgttgcgc gctgcgagcg
     1621 tgaacccgac cgagctcttc tagccgaaag ccgcgccgag agcggggagc tcatgataaa
     1681 agacgcgacg aggaactatg ctgcgactcg tcttgggtaa tctgaactat tcgtcttgga
     1741 gcatgcgccc gtggctcgcc ctgaagcgcg ccggagcaaa gtttcaggtg cacgacgtcg
     1801 ggctgcggac cgcgccggat tggaaagagc gcatcctctc cttttccgga gccggcaagg
     1861 ttccaatctt ggtcgacggc tcactctcga tccacgaatc gctcgcgatt tgcgaatacg
     1921 tcgccgagct ccatccagaa gccaagctct ggcccgcgga catgaagctg cgcgcgcgag
     1981 ggcgggcgat ttcttgcgaa atgctgagcg gcttctcggc tttgcgctcg tgcatgagca
     2041 cgaacctgcg ggcgcgggcc gagaagaccc cggacgcgcc cggcgtgcgc gccgacatcg
     2101 cgcgcatctt cgacatttgg accgcgtcgc tcgagagcac cggggggccc ttcttgctcg
     2161 gcgagttcag catcgccgac tgcatgtatt tcccagtcgc gacccgcttc cgcacctacg
     2221 gcgtcgggct gccagagcgc ctggcgcgct actcggcggc gctgttcggc actcccgaag
     2281 cgcgggagct cgagcaaatc gctgccacta ctcctgcgat tccggaatac gacgtcgcgc
     2341 tccggccgct ctgagcccag cgcttgcgcg tcacgctgtg agcaccgcgc gaccgctcgg
     2401 cgcttgcgtg tcacgctgcg agcaccgcgc gaccgctcgg cgcatcggct cgcgtcactg
     2461 ggcgtgacgc gagcaatcgg tccgcggggc gcagcgccca cgacgcgatc gcgagaccga
     2521 gaatgacgag cggcgtcatg atcttgccgg ccgcgtcgcc gaccgcggcg tgggaaaccg
     2581 ccgcacccgt caggtcgaag aagatccccg cgtaggccca ctctttcaac cgaggaaggc
     2641 gcggtgcgag cagcgcggcc gcaccgagta gcttccagac gccgagcaag ctcgcgacgt
     2701 aagccggata gccgagtttg tgcatcgcat cgatcaccgc gggagcggaa gtcacgtcgg
     2761 cggcgccacc ggccacgaac gcgaacgaaa cgagtgcggt ggtcgtaaaa taggcaattt
     2821 tgcgcgtctt catcgagatt tctccgtcgt ttggcgacga cgatgaatct gactctcgac
     2881 ctcactcaca acaatgcgtc aacgcttggc atcatttgat acaaattgat cccaatgcag
     2941 gacccgattg aaacctccga gctgctcgcg ttcgtgaaga ccgtggagac gcactcgttg
     3001 tcgcgcgcag ccgcagagct cggcgttccg cgcgctacga tcagccggcg cctggctcgg
     3061 ctcgaggagc gcctaggcgc tcggctgctg cgccgcacca ctcgtagctt ggtgctcacc
     3121 gacgctggtg aaatgctgca tcgtcacgcg cgcatcgtgc tcgacgcggt tgctcaagcc
     3181 gaaggctcgg tgcgacaagc agacccagtg gtgcgcggcg cactgcgcgt ttctgctccg
     3241 tcgctgatta acgaggcttt cgtcgacatg gtcgcggact tcgtcagatc tcaccccgcc
     3301 gtccagctgc acctacacat ctccagccag cacgtcgacc tgcgccgcgg cgggcacgac
     3361 gtcgcgctga gagcatcgag tgagctcgag cccgggctcg tcgcccgtac tttgatgcgc
     3421 accaccctca tcgctgtcgc cggtccgagc tacttgaagc aatacggcgc tcccaagaac
     3481 ggcgacgacc tcgccaatca tcgctgcttg gtgggcttcg cgcggggcga gctaccgcag
     3541 actcactggc cgctcgtgcg tggcggcaag ctcccggtgg aaggcgcgtt cttcaccaac
     3601 gagctgccgg tgttgttgga tttcgcgctg cgcgggctcg ggatcgcctt gattccggtg
     3661 aatgtcgctc aaccttatct cgacagcggg gaactcgtgc acgtgctgtc gggagaggta
     3721 ggcgccgaaa gtcgattttc gatcgtctat ccagaacgcg aattcgttcc accccagctg
     3781 cgagcgttca tcgatgcggt cgtggcgtgg cggcgggttg gcgaagcgat atccccaccc
     3841 agggtcggcg gaggctccgc gaagcgcaag aagcggaacg tcaaaaatcc gaagccacag
     3901 cctcggcgag gcaccggctc gtgacgcag
//
LOCUS       DASTKS010001133         7163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1743066, whole genome shotgun sequence.
ACCESSION   DASTKS010001133 DASTKS010000000
VERSION     DASTKS010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743066"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFQ35_24030"
     CDS             complement(<1..717)
                     /locus_tag="VFQ35_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET9933803.1"
                     /translation="MNHIDKAGTFKLGHRAVQRVGYGAMQLAGPGVFGPPKDRDAALA
                     VLREAVTSGVNHIDTSDAYGPHVTNQLVRAALHPYPNELVIVTKVGARRGPHGSWDPA
                     FSAEDLTSQVHDNLRNLGLDTLEVVNLRAMFDPLRPTEGSIEAPLATLLELQAQGKLR
                     YIGLSNVTAKQVAEGRAMTEIVCVQNHYNVAHRSDDPLIEELSAARIAYVPFFPLGGF
                     SPLQSATLSQVANQLGATTMQ"
     gene            782..1762
                     /locus_tag="VFQ35_24035"
     CDS             782..1762
                     /locus_tag="VFQ35_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9933804.1"
                     /translation="MANNRIQLDDIQVFAAVARAGGFREASRVSGMSASSASEAVKRL
                     EAELGVRLLHRTTRSVAPTEAGAGLLARLSPALAEIDSALDVVRGSRDQPAGTVRLNV
                     PTIAARVVLPRLVPSFLAAHPQIRLEVTAEDDLVDILAAGCDAGIRYEETLDKDMIAI
                     PIGPREQCYALAASPAYLERHGYPAHPRDLLDHACLRLRFASGALVPWEFERSGKTVK
                     VEPSGPLVATLGNAADLLVAAALADAGILYLFEDWLRPHFESGALKPILERWWLRFSG
                     PFLYYPSRSHMAAPFRAFVDFVRNHRTSVSATRVSHATVSAGVSRRRRRK"
     gene            complement(1851..2816)
                     /locus_tag="VFQ35_24040"
     CDS             complement(1851..2816)
                     /locus_tag="VFQ35_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9933805.1"
                     /translation="MSAIVQDRYGSADVLELRQIARPRPRADEVIVRVRAAGVDFGVW
                     HMMEGMPYAVRLVSGLRRPRNPVRGLELAGIVEEVGEHVTTFARGDEVFGIGEGSFAE
                     YARASQSKLVHKPSSLGFDEAAAVPVSATTALVGLRAANVGPGQNVLITGAGGGVGSY
                     AVQLAHAMGAEVTGVCSTSKVDFVRALGATRVIDYTRENISAGDPTYDAVIDLAGRRS
                     VSELRRALGSSGTLVILGGEGGGRWLGMGRQVWSQIVGLASRQKFRSPLALVNQADLR
                     LLTEMLATGKLRPVVERRYQLSEVPTAVRALAAGHSRGKSVITIG"
     gene            3067..4629
                     /locus_tag="VFQ35_24045"
     CDS             3067..4629
                     /locus_tag="VFQ35_24045"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9933806.1"
                     /translation="MGAESFKWEFFIAHAGADIESAEELYGYLNSAASVFLDKACLLL
                     GDDWDRKLAAAQRASLVTLVLVSSQTDQAYYQREEIAAAIDLARRNENNHRVIPIYLD
                     GATEPPYGLRLKHALRLGAELSMSDAAAQLLAELPRIKALAGDAHDVPVVPSPAPVLG
                     GKVALSLAVDPTVASGEAEDEALALAYEYGQDDKNTIVISPQLGYLESIACGGPIAPT
                     RFDWVPFSWDFPKLDFKVINNSKETILLSDIVFEVEESKLDPTPVLIVKPDIYQSNAL
                     HFRLINEGWGPAVGLKACFNLTPTDARNSAKPTYGEPYPYEVQVGDVDEDANVDISSE
                     LVKAGVDLRGLADLGVMTQRDGDVSTFVNEKGVERTFDEAQLWAARQQLFGRFTSGGA
                     LVSGELRYTARTVDGAAEERRVKFWTVVWLFNENRKGKPRPPSYEYATKFKVEGINYK
                     RQLSISQEVKAGDTDRFLVKVGFDKSSRHRFRARLLYNGGREVVSANVLLYGFAPRSG
                     ISHLRDENSKKR"
     gene            4882..6984
                     /locus_tag="VFQ35_24050"
     CDS             4882..6984
                     /locus_tag="VFQ35_24050"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HET9933807.1"
                     /translation="MGTQKAVVFIASRFGEFSELRKALVLEIAATKPMTPVDLNDGYA
                     RHEPPLAECLARVRGADLMVLLVGDSYGPNAPGKSKSFTHLEYDEAARDGSKTRLLVY
                     RFSSSGVREGDRRVPDEWLRELEEKHTPKRLGVVTDPKARASEITKHLFAALYDLRFD
                     EPSEDEDEADLDAAEDAFDDAEIDSLDAIDAEARGETGDPASELPAGSETRRPAAAAS
                     IEQRTEAERATRLGEYGVAIQHLRKALELRPLDGLANFLLARLYVVLNREDRLGEAVN
                     LAERAARIAGDDQLPYRAARSHLLAARALTAMRHFPDAHRAVDAALEEAPDFAKAHIE
                     RARVFCAEEKASQAVAAVREGFRHHSRSLQDALRDPHLEEIRSSLRELLREQTAVWQR
                     DVERLLELERWLAEQVDPSLPVAPAGIPANTNLQNLRRLGRASVARQRELVSTLVLAA
                     KRQYLDSLPETRGSAAGKLVAEIKHGEQQALELRAREREAAQILEAAQKVPSWFSAQV
                     IFSLIIITGSAVMVRSVWAACVSFGIGSVFAVQGLWNSWQRRKSAAVNREAARGQHSA
                     AADLCEDSEARLASTKREFAELQSAGTRARDRAHAAVEAFELRALSMPSRYQPFSTLR
                     RARPQKLVRASAKSVEQFEQSYRRRIEKDAAPSWLGEFDASDRFALYRVVSVSPNAIR
                     LSRDRVYDAALGSAAAAE"
BASE COUNT         1244 a         2239 c         2379 g         1301 t
ORIGIN      
        1 ttgcatcgtc gttgcgccga gttggttcgc gacttgcgac agggttgccg attgcagcgg
       61 cgaaaaaccg ccaagcggaa agaacggcac gtacgcgatg cgcgcggcgg aaagctcctc
      121 gatgagcggg tcgtcactcc ggtgcgcgac gttgtaatgg ttctgtacgc acacgatctc
      181 cgtcatggcc cggccctcgg ccacttgctt cgcggtgacg ttactgagcc cgatgtagcg
      241 tagcttgccc tgggcttgca actcgagcag cgtcgcgagc ggcgcctcga tcgagccttc
      301 agtgggccgc agggggtcga acatggcgcg caggttgacg acctcgagcg tgtcgaggcc
      361 gaggttccgg aggttgtcgt gcacctggct cgtgaggtcc tcggcggaga acgcggggtc
      421 ccacgagccg tggggaccgc gtcgtgcgcc gaccttcgtc acgatcacga gctcgttcgg
      481 ataggggtgg agcgctgcgc gcacgagctg gttggtgacg tgcggaccgt atgcatcact
      541 cgtatcgatg tggttgacgc cgctcgtgac cgcttcgcgc aaaacggcga gggccgcgtc
      601 ccgatccttc ggcggtccga agacccccgg cccggcgagt tgcatggcgc cgtatcctac
      661 gcgctggacg gctcgatgcc cgagcttgaa cgttccggct ttatcgatgt ggttcatgcg
      721 gtgagcctaa gtcgcggaac ggtgtacgat tagtatgtaa aatcggaaca ggctgtgcgg
      781 catggcgaac aatcgcatcc aactcgacga tattcaggtg ttcgccgccg tagctcgcgc
      841 gggcggcttt cgtgaagcca gtcgcgtgag cgggatgagc gcatcgagcg cgagcgaggc
      901 ggtcaaacgt ttggaagcgg agctcggcgt tcgcttgctc catcgcacga cgcgcagcgt
      961 tgctccgacc gaagcgggcg cggggctcct cgctcggctc tcccccgcgc tcgccgaaat
     1021 cgattccgcg ctcgacgtcg tgaggggctc gcgcgatcag cccgccggca ccgtgcggct
     1081 caacgttccc acgatcgccg cgcgagtcgt ccttcctcgc ctcgttccat cgttcctggc
     1141 tgcccacccg caaatccgcc tcgaggtaac cgcagaagac gatctcgtcg acatcctcgc
     1201 cgcgggttgc gacgcgggaa tacgctacga ggagaccctc gataaggaca tgatcgcgat
     1261 tccgatcggc ccgcgcgagc agtgctatgc gctggccgcc tcacccgcct acctcgaacg
     1321 tcatgggtat cccgctcatc cgcgagacct gctcgaccac gcctgcctgc gtctcaggtt
     1381 tgcgagtggt gcgctcgttc cgtgggagtt cgagcggtcc ggcaagacgg tgaaggtgga
     1441 gccgagtggt ccactcgtcg ccactttggg caacgccgct gaccttctcg tcgcggcggc
     1501 tctggcggac gccgggatcc tctacttgtt cgaggactgg cttcgcccgc atttcgagag
     1561 tggcgcattg aagccgattc tcgagcgttg gtggctgcgc ttttcgggac ccttcctcta
     1621 ttacccgagc cggagtcata tggcggcgcc tttccgcgca ttcgtcgact tcgtccgcaa
     1681 ccatcggacc tcggtgagcg cgacccgcgt ctcccacgcg acggtaagcg caggtgtttc
     1741 acgccgacga cgcaggaagt agcttgcgcg attccgcgtt cagaaatcca cggccgtgct
     1801 agtcgcgtaa acacccgctc gccctgccgg tgcgccaggc gagacggcac tcaacctatc
     1861 gtgattacgg acttgccccg cgaatggccg gcggccaacg cacggaccgc ggtcggcacc
     1921 tcgctcagct gatagcggcg ctcgacgacc ggtcggagct tgcccgttgc cagcatctca
     1981 gtcagcaatc gcaagtccgc ctggttcacg agcgcgagtg gcgagcggaa cttctgtcga
     2041 ctcgcgagcc cgacgatctg cgaccacacc tggcgaccca tgccgagcca gcgtccgccg
     2101 ccctcgccgc cgaggatgac cagcgtgccg ctcgagccaa gtgcacgcct cagttcggac
     2161 acgctgcgcc gacccgcgag atcgatgacg gcgtcgtacg tggggtcgcc agcgctaatg
     2221 ttctcgcgcg tgtagtcgat cacgcgcgtc gcgccgaggg cacggacgaa gtcgaccttc
     2281 gatgtgctgc acactccggt cacctccgcg cccatcgcgt gcgcgagctg taccgcgtag
     2341 gaaccgacgc cgcctcctgc accagtgatc aacacgttct gaccgggccc aacgttcgcc
     2401 gcgcgcaggc cgacgagcgc ggtggttgcg gacacgggta cggctgcggc ctcgtcgaac
     2461 ccgaggctcg acggcttgtg tacgagcttg gactgcgagg cgcgcgcgta ctcggcgaac
     2521 gagccctcgc caatgccgaa gacctcgtca ccgcgcgcaa atgtggtgac gtgctcaccc
     2581 acctcctcga ctatcccggc gagctcgagg ccgcgcacgg ggttgcgcgg acggcggaga
     2641 ccggagacga ggcgcacggc gtaaggcatc ccttccatca tgtgccacac cccgaagtcg
     2701 acgcccgcgg ctcggacccg cacgattacc tcgtcggcac gcggccgagg ccgcgcgatt
     2761 tgtcggagct cgagcacgtc tgccgaacca tagcggtctt ggacaatggc gctcatgagc
     2821 gaatcggtcg cacgatcggt agcaggaact tggtgggtgt tcatcatgaa gccatcctat
     2881 cggcgcccac gggtggagga ttgaacgaaa gggctgcccc gtggcatggc gcttcgcttg
     2941 ccggcagcgc gagggatgag cagctaggct cgtcagccgt cttccgtcgc gcgcgccgtc
     3001 gcgcggtcat ggtgccgcct ccgtgggtat gttggcgcgt tccgtcgctt cgagtagatt
     3061 cgccgcatgg gtgccgagtc gttcaagtgg gagttcttca tcgcgcacgc tggcgcagac
     3121 atcgagtccg ccgaagagct ctatggttac ttgaacagcg ccgcgagtgt attcctagac
     3181 aaggcttgcc tcctcttggg cgacgactgg gaccgaaagc tcgccgccgc tcagcgcgcg
     3241 tcgttggtca ccctggtgct ggtgtcgagc caaacggatc aggcgtacta ccaacgcgag
     3301 gagatcgccg ccgccatcga cctggcgcgt cgcaacgaaa acaatcatcg cgtcattccg
     3361 atctacctcg atggagctac cgagccgccg tacgggttgc ggctcaagca cgccttgcgt
     3421 ctcggtgccg agctgtccat gagtgacgcg gcggcgcagt tgctcgccga gctaccgcgc
     3481 atcaaagcgc tggccggcga cgcgcacgat gtaccggtcg tcccgtctcc cgctcctgtc
     3541 ctcggcggca aggttgccct cagccttgcc gtcgatccga cggtcgcaag cggcgaggcc
     3601 gaagacgaag cgctcgctct ggcatacgag tatggccagg acgacaagaa cacgatcgtg
     3661 attagtccgc agttggggta tctcgagtcg atcgcctgtg gcggaccgat cgcgcccacg
     3721 cggttcgatt gggtcccctt cagctgggac ttcccgaagc tcgatttcaa ggtaatcaac
     3781 aatagcaaag agacgatcct gttgagcgac atcgtattcg aagtcgagga aagcaagctc
     3841 gacccgacgc cggtgttgat cgtgaaaccg gacatctatc agagcaacgc gctccacttt
     3901 cgtctaatca acgagggctg gggccccgcg gtggggttga aggcttgttt caacctcact
     3961 ccgaccgacg ctcgtaactc ggcaaagccg acttatggcg agccctaccc gtacgaggtc
     4021 caagttggcg acgtcgacga ggacgcgaac gtggacatct cgtcggagct cgtgaaagct
     4081 ggagttgatt tgcggggcct cgccgacttg ggggtcatga cccagcgaga tggtgacgtg
     4141 tcgacgttcg tcaacgagaa gggtgtcgag cgcacgttcg acgaagcgca actgtgggcg
     4201 gcgcgacagc agctgttcgg gcggttcacg tcgggcgggg cgctcgtcag cggtgagctc
     4261 cgctatacgg cgcgaaccgt cgacggcgca gccgaggaac gccgcgtgaa gttctggacg
     4321 gtcgtctggc tcttcaacga aaaccgcaag ggtaagccgc gcccgccgag ctacgagtat
     4381 gcgaccaagt tcaaagtgga gggcatcaac tacaagcgcc aactctcgat ctcgcaagag
     4441 gtgaaagcgg gcgacacgga tcgcttcttg gtgaaagttg gcttcgacaa atcatcgcgt
     4501 caccgttttc gcgcgcgcct cttgtacaac ggtggccgcg aggtcgtgtc cgcgaacgtg
     4561 cttttgtacg gcttcgctcc gcgctccggg atcagtcatt tgcgcgacga aaactcgaag
     4621 aagcggtgat acgtttcggc tgcgagcggc atccccggtt ggagctgcct agagctttcg
     4681 ctgaaagctg gctcgggcgc gatcacgcga gcgcgcgctc gggggagccg aggcccgtgc
     4741 agcgcatggt catttgttcg agatcgacga ctctcgtccg aacgtcgagc gtgccgcccg
     4801 tggtgctgtg agcttgccat tgtgtttcga ggtccgcgcg agtaacgctc gtttcaagag
     4861 ggaactccag gaggcgaact cgtgggaact cagaaagcag tcgttttcat cgcgtcccgc
     4921 tttggggagt tttcagagct gcgcaaggcg ctcgttctgg agatcgccgc gaccaagccc
     4981 atgacgccgg tcgatttgaa tgacggctac gctcgacacg aaccgccgct cgcggagtgc
     5041 ttggctcgcg ttcgcggcgc cgacttgatg gtgctgttgg tcggcgatag ctatggtccg
     5101 aacgcacccg ggaaaagcaa gtccttcacg catctcgagt acgacgaggc ggcgcgagat
     5161 ggttcgaaga cgcgattact ggtgtatcgc ttcagctcgt cgggggtgag ggaaggcgat
     5221 cgacgcgtgc ccgacgaatg gctgcgcgag ctcgaagaga agcacacgcc gaagcggctc
     5281 ggcgttgtaa ccgacccgaa ggcgcgcgcg agcgagataa cgaagcacct ctttgccgcg
     5341 ctctacgacc tgcgcttcga cgagccgtcg gaggacgagg acgaggcgga cttggacgcg
     5401 gcagaagatg cgttcgatga tgccgaaatc gattctctcg atgcgatcga cgcagaggca
     5461 cgaggcgaaa cgggggaccc cgcgagtgag ctccccgccg gcagcgagac gcgaaggccg
     5521 gccgcggctg cgtcaatcga gcaaaggacg gaagccgaac gggcgacgcg gctcggtgag
     5581 tacggcgtcg cgattcagca tctgcgaaaa gcgctggagt tgcggcccct cgacggcctc
     5641 gcaaacttct tgttggcgcg cctatacgtc gtcttgaacc gcgaggatcg gctgggtgag
     5701 gcggtgaacc ttgccgagcg cgcggcgcgc atcgcaggtg acgaccagct gccctaccgc
     5761 gcggcgaggt cgcacttgct agcggctcgg gcgctgaccg cgatgcggca ttttccggac
     5821 gcgcaccggg ccgtcgacgc cgcgctggaa gaagccccgg atttcgcgaa ggcgcacatc
     5881 gaacgggcgc gcgtcttctg cgccgaggag aaggcctcgc aagcggtagc ggccgtgcgc
     5941 gaaggttttc gccaccatag ccgaagcctg caagatgccc ttcgcgatcc gcatctcgaa
     6001 gagattcgtt ctagtcttcg cgagctcttg cgggagcaga cggcggtgtg gcagcgcgat
     6061 gtcgagcgct tgctggagct agaacgctgg ctcgcggagc aagtggaccc gtcgcttccg
     6121 gtggctccgg cgggcatccc ggcgaacacg aatttgcaga acttgcgccg cctcgggcga
     6181 gcatctgtgg cgcgtcaacg agagctcgtg agcactttgg ttttggccgc caagcgccag
     6241 tacttggact cgctgcccga gacgcgcggg agcgctgccg ggaagctcgt cgcggagata
     6301 aagcacggtg aacaacaggc gctcgagttg cgcgcccgtg agcgcgaagc cgcgcaaata
     6361 ctcgaagccg cgcagaaggt gccgtcgtgg ttcagtgcgc aggtgatctt ttcgctcatc
     6421 atcatcaccg ggagtgcggt gatggttcgc agcgtgtggg cagcgtgcgt ctcgttcggc
     6481 atcggctcgg tgttcgcggt tcaggggctg tggaactcat ggcagcgtcg aaagtccgcg
     6541 gcggtgaacc gcgaggccgc gcgtggacag cattcggcgg cggcggatct ctgcgaagat
     6601 tccgaggcga gactcgcttc taccaaacgc gaattcgcag agcttcagtc ggcaggtacg
     6661 cgggcgcgag accgagcgca cgccgcggtc gaggccttcg agctgcgcgc tttgagcatg
     6721 ccgtcgcgat accagccctt ctcgactttg cgtcgtgctc ggccgcagaa gctcgtccgc
     6781 gcctcggcga agagtgtgga gcagttcgag caatcttatc gccgtcgcat cgagaaagat
     6841 gcggcgccgt cctggctggg ggagttcgac gcctccgatc ggtttgcctt gtatcgagtc
     6901 gtttcggtat cgccgaacgc gattcggttg tcccgagatc gagtctacga cgctgctctt
     6961 ggctccgctg ctgccgccga gtgactgcgc gactcggttc acatccggcc ggcaggcgcc
     7021 gaagctcagt cgcccttgcc gttgcgcatg atgatgccgt cgagaccaac cgcccaaacc
     7081 tcgctcgaac tagcgcccca gactgcgttg aggtgcggct gtgcgatggg ataaagtgca
     7141 gcattttgcc aggcgccatg cat
//
LOCUS       DASTKS010001134         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1968763, whole genome shotgun sequence.
ACCESSION   DASTKS010001134 DASTKS010000000
VERSION     DASTKS010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968763"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFQ35_24055"
     CDS             <1..1068
                     /locus_tag="VFQ35_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9933808.1"
                     /translation="RTGFGPGFLVGGLPRSFPRSFRGPGPRRLPTPESRGRKRPFVIE
                     GDEYDTAFFEKTAKFLHYRPEVAIITSIEHDHIDIYPSLAAYLEAFERFVTLLPEDGL
                     LVANAADPLVRSVAEKSRAEVAFFALEGEDTGGVAPHWLAAPALSGPTGTAFDLFAGG
                     MLAGRFATTLPGRHNIKNAVAAIAAAAQGFGARLKELGTALGSFEGVRRRQELLGSPG
                     GVAVYDDFAHHPTAVRETLDALRARHPSGRLIAVFEPRSATACRKLHQSEYPRAFGAA
                     DLVLFAPLGRTNLSSDEMLDLDRVVEDIRAQGKHAERCSTIDAVGARVKELAKPGDIV
                     ALLSNGAFGGLPRRLATELSS"
     gene            1097..1588
                     /locus_tag="VFQ35_24060"
     CDS             1097..1588
                     /locus_tag="VFQ35_24060"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HET9933809.1"
                     /translation="MTCPCGKGESLETCCGPFIDGSKLPETAEELMRSRYTAFATGKV
                     DYILSTHDPERRGDVDEQGAADWSKNSEWLGFELISTEKGGPADDAGVVEFVAKYKIK
                     GVTLTHRERSIFRKHNGHWVFVDGQMVNSPPVKRTEPRVGRNDPCPCGSGKKYKRCHG
                     AAA"
     gene            1818..4694
                     /locus_tag="VFQ35_24065"
     CDS             1818..4694
                     /locus_tag="VFQ35_24065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9933810.1"
                     /translation="MKAPPAVPARAPASPPTKPPPKPLAKPPPKPLPKPAKPVETAPR
                     AHESARATLDPSVLVVGADDVFLSALKLALARHRVHVESSSLEQAVETVVVAAPDLIL
                     LANEAARDAGHGLLAKLTSSPVSSVIPVVILGGDSALDARLRAFRHGATAVIPRTASI
                     DAVAETIGKLAREIPERGGDALGEVGEATLEEFVGALTKELRSGILSLDTGEEKAPVR
                     LVLGSGRPLAAFIDEFVRRARQHVVRAEPLRYEFDDRAGGTVQLLPEESGASEPPPGN
                     IRGLRLLLADNQPSRADLIAQALRARGAEVVIGSLDPNEPELRRIRQLDPEVILIGER
                     ELEGAGYALLRRMKRDTRLRWASLLVVRWDEISGPTSGVPAIERLTGPLATLAEADRG
                     LCDRAELGGAFDARLEVNGPARCLRALAASGRSLRLTLHNNRLTVSVDISDGLIVGAT
                     ANAPSGETWGGAQALAAFMVLSSGRIHVEPAEQPATTNLMAEVDAALSLAESEPAPIA
                     PSLPANAIPSEAPLASSAVEPQIPRAAPVPRGLDVDPLAQTAAAIDALAITAPVALPP
                     TVVGPAEVPPAAAPPEAPPAPVAKRRLTPAKLIESFPRLEAAARLSEHPRFRVKGISP
                     PIAALLVAIAVVQGLLAAVIYKYVHRTAPVDARDLASAVAQSAATAAAPAVPATPAPG
                     ATPAPSAAATPLASSAPTPSASTELPRDEQPAPAPSCDELLKDSPGGGENPGAAFEQI
                     KLARKALVQGKSDDAERAYCKAVRWDPQNIGYYFELAQLLLIRRDGAAAAEWAQKGAR
                     LDPANTRGQSLVGDGLARIGDFDGARRAWYAAASVNTPSAQEVEQLFLRAQKEAEQAL
                     ATHDYMRAERFFRRAAVLDPKNASAQRGLASSLLRIGEAKNALAWARRAEQLAPKDAT
                     VQLTLGDALLATGDDAGARQAWTEAERLGYPDARRRLERLAKRP"
     gene            4704..5120
                     /locus_tag="VFQ35_24070"
     CDS             4704..5120
                     /locus_tag="VFQ35_24070"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET9933811.1"
                     /translation="MTAEAETSLFAELGGEPALRRIIDTFVDRVFDDIMIGFLFQRAN
                     RERIKEKEFEFAAEFLGAPVEYSGRSLEDAHRRHRIFDGQFQRRLTILRNTLDELSAP
                     ARVRDALLAHTVSLRARVVAGDCDEPVAAIPGSRPE"
     gene            5304..6074
                     /locus_tag="VFQ35_24075"
     CDS             5304..6074
                     /locus_tag="VFQ35_24075"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HET9933812.1"
                     /translation="MAQPLPNESWEGEATEPDAPQASQSSSPEARPRARIIGERERTE
                     ANPEVAEAGAATPRLALQGGGRSLGTFRNTYYDFPSEADFDGAKVPIKSARCETLAEV
                     PRGFFESVCVQGSGTLRSGATVSFAKRDCACAEICPRTDQKICFDSLDPAAYPWGRGA
                     TGKAITPLLTVAVDTDVIPLGTALYVPELDGIPRDDAGTTHDGCFIAQDRGVRVKGKH
                     VDFFTGHASLTALWNRLVPSNKGVTVVLESPRCARAAR"
     gene            complement(6078..6602)
                     /locus_tag="VFQ35_24080"
     CDS             complement(6078..6602)
                     /locus_tag="VFQ35_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933813.1"
                     /translation="MAALACADMDTIAALNVNVSKKAPQLTKIHVSIRQGGKEVAKDY
                     DMLPTRTEGEGDAKMTFTAENFFERIKLDGFSDGKAMADVEAFAATGSFDHPPAVDFD
                     VRENGATAVYVKIMTPEERAAASAGGAGGGGAGGNGGASAGNGGAAAAGGGAGGAANG
                     GNGGAAAGNGGVGG"
     gene            complement(6865..>9072)
                     /locus_tag="VFQ35_24085"
     CDS             complement(6865..>9072)
                     /locus_tag="VFQ35_24085"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9933814.1"
                     /translation="IGQQENNMEVYIRGIGSNNNTELGDPATATHIDGVYIPRPRGVG
                     AMFFDLERVEINRGPQGTLRGRNATAGSLNIVTAKPQYKEWDANASVGLGNFSQREVR
                     GMLNIPIGDRLALRLAASGETRSPFYKNAGPVKWLQGSESADTLAYRAALSWNPVDAL
                     KIIVKHDYMQEGGTGSTGSNVNGALTAGLTPEEIPDVRSVVYRTPQGREDSKHWGITG
                     DIGVDLGVLNIGYVGSYRYLDYNQINSGQAGVDFPGRTDYDVDNSGTTYWHQTSKSVT
                     QELRLYAPDSARFRWSVGGFFFNEEQKTHFATTNDKSNGFFGVEFTMPKMHSRSWAGF
                     ADGTFDILKELRVTAGVRGTTEEKSRDGIGNVYSFSGISESQRFGTDGFAYADQGRTL
                     YTITDASKASPFDVFRNGIARFGQRDTLDKALQAQGVGFSGSANEQHGSYSDKFFDFR
                     VGVDGNLTKSNLLYALFSTGHKSGGFNDNVTFPSGATLAPKYKPEVVYAIEVGSKNEL
                     LNKAVIANASAFYYSYQDLQLQQVVAIGDPMDPTRTPPASAVRSNAAQSRVMGLELEG
                     VGRLPAGFSLRLSGLLMDARFTEGDIADTRISYDPATQGHVNLKDKFLPRAPQLSVNY
                     GITQNIQTSIGYFDWVVNFQTKTKYYMTPFNGDGFDSKGNFDPNTSDVVKGFTRFDVG
                     AGFTHVSGKVRFEGFINNVTDKAYMTSLINTPGLNLRFYNPPRQMGLRLTVFM"
BASE COUNT         1524 a         3053 c         2975 g         1520 t
ORIGIN      
        1 cgcaccggct tcggaccggg ctttttggtt ggcggcctgc cccgtagctt cccgcggagc
       61 tttcgagggc ccggaccgcg acgcctgccc acccctgaat cgcgcgggcg aaagcgtccc
      121 ttcgtgatcg agggggacga atacgacacg gcgttcttcg agaagacggc gaagtttctc
      181 cactaccgcc ccgaagtcgc gatcatcacg tccatcgagc acgatcacat cgacatctat
      241 ccgtcgctcg cagcgtactt ggaggcgttc gagcgcttcg tcacgctctt gccagaagat
      301 gggctcttgg tcgccaacgc ggccgatcct ttggtgcgaa gcgtggcgga gaaaagccgt
      361 gccgaggtcg cgttcttcgc gctcgaaggc gaagatacgg gcggcgttgc accgcactgg
      421 ctcgccgcgc cggcgctcag cggcccgacc ggaaccgcgt tcgatctctt tgcggggggg
      481 atgctggcgg ggcgcttcgc gacgacgctg ccgggacgtc acaacataaa gaatgcggtg
      541 gccgccatcg ccgcggccgc gcaaggcttc ggagcgcgcc tgaaggagct cggcaccgcg
      601 ctcggaagct tcgaaggagt gcgtcgccgc caagagctcc tcgggagccc gggcggggtc
      661 gccgtgtacg acgacttcgc gcaccacccg acggccgttc gcgagacgtt ggacgcgctg
      721 cgcgcgcggc acccaagcgg gcggctcatc gccgtattcg agccgcgcag cgcgaccgcc
      781 tgccgaaagt tgcaccagtc cgagtaccca cgggcgttcg gtgccgccga tttggtccta
      841 tttgccccgc tcgggcggac caacctatcc agcgacgaga tgctcgatct ggaccgcgtc
      901 gtcgaagaca tccgcgcgca gggaaagcac gccgaacgct gcagcaccat cgatgcggtg
      961 ggggcccggg tgaaggagct cgccaagccg ggcgacatcg tggcgctgct ctcgaacggc
     1021 gctttcggcg ggttgcctcg acggctggcg accgagctct cgagctgata tacgtcgcgc
     1081 cccacgaggt aatgaagtga cctgtccctg cggaaaaggc gaatcgctcg agacctgctg
     1141 cggcccgttc atcgacggca gcaagctccc ggaaacggcc gaagagctga tgcgctctcg
     1201 gtacacggcg ttcgcgacag ggaaagtcga ttacattctc tcgacgcacg atccagagcg
     1261 ccgaggcgac gtggacgagc aaggtgctgc ggattggtcg aagaactcgg aatggctcgg
     1321 cttcgagctg atctcgaccg agaagggcgg ccccgcggac gacgccggag tcgtagagtt
     1381 cgtcgccaag tacaagatca agggcgtgac gctcacccac cgcgagcgct cgatcttccg
     1441 caaacacaac ggtcactggg tgttcgtcga cggacagatg gtcaatagtc cccccgtcaa
     1501 gcgcaccgag ccgcgcgtcg gccgcaacga cccttgccca tgcggcagcg ggaagaagta
     1561 caagcgctgc cacggcgctg ctgcctgacg actcacagcc gcgtcgcgcg gaatccgcgg
     1621 gtcgcatgtc ggcgtctgcc gcttagaaat gcggagatcc ctcaggtatg cgttcgtcgc
     1681 agggctcccc accaacgctc gaaaaatccg agctggtcgt gacggctggg ctgggggatg
     1741 atgaacgctc gatgagtacc ggtgacgccc ggtcgcgagt tccccggccg ggagtttcgc
     1801 aggcacccac ggtgtcggtg aaagctcctc ccgcggttcc cgcgcgcgca ccggcgtcgc
     1861 caccaacgaa gccgccgccg aagccgctcg ccaaaccgcc gccgaagccg cttccgaagc
     1921 ctgcaaagcc cgtcgagacg gcgccgcgag cgcacgagag cgcccgcgca acactggatc
     1981 cttcggtgct cgtcgtgggc gcggacgacg tcttcctgtc ggcactgaag cttgcgctcg
     2041 cgcgtcaccg cgttcacgtg gagtcgagtt cgctcgagca agcggtcgag accgtagtcg
     2101 tcgccgcgcc cgatttgatt ctgctcgcga acgaagccgc gcgcgatgcc ggccatggtt
     2161 tgctcgccaa gctcacgagc tcgccggtca gctcggtgat cccggtcgtc atcctgggcg
     2221 gcgactcggc gctcgacgcg cgacttcggg cgtttcgtca tggcgcaacc gccgtcattc
     2281 cccgaaccgc gagcatcgac gcagtcgcgg agacgatcgg caagctcgca cgcgagattc
     2341 cggagcgcgg tggcgacgcg ctcggcgagg tcggcgaggc cacgctcgaa gagttcgttg
     2401 gcgcgctcac gaaggagctc cgctccggga tcttgtcgct cgacacgggc gaagagaagg
     2461 cgccggtgcg cctcgtgctc ggcagcgggc gccctctcgc tgccttcatc gacgaattcg
     2521 tgcggcgtgc gcgtcagcac gtcgtgcgcg cggagccgct acgctacgag ttcgacgatc
     2581 gcgctggcgg aaccgtgcag ctcttgcccg aagaatcggg cgcgtcggag ccgcctcccg
     2641 gcaatattcg cgggcttcgg ctgctcctcg ccgacaatca accgtcgcgc gccgatctga
     2701 tagcgcaagc cctccgcgcg cgcggcgccg aggtggtcat tggctcgctc gatccgaacg
     2761 aacccgagct gcggcgcatt cgtcaactcg atcccgaggt catcttgatc ggggagcggg
     2821 agctcgaggg cgccggctac gcgctcttgc ggcggatgaa gcgcgacact cgtctgcgct
     2881 gggcgtcgct gctcgtcgtt cgctgggacg aaatcagcgg ccccacttcg ggagtgccgg
     2941 cgatcgagcg cttgaccggc cctctcgcga cgctcgccga agcagaccgg gggctttgtg
     3001 atcgcgccga gcttggcggt gccttcgacg cgcgcctcga ggtgaacggc cccgcgcgct
     3061 gccttcgtgc gctcgccgcg agcgggcgct cgctacgcct cacattgcac aacaatcggc
     3121 tcacggtgtc ggtcgacatc tcggacgggc tgatcgtggg cgcgaccgcg aacgcgccga
     3181 gcggcgagac gtggggcggg gcccaagcgc tcgcggcctt catggtcttg agcagcggac
     3241 gcatccacgt cgaaccggcg gagcagccag cgacaaccaa cttgatggcg gaggtcgacg
     3301 cggcgttgag cctcgccgaa agcgaacccg cgccgatcgc tccgtcgttg ccagcaaacg
     3361 cgatcccgag cgaagcaccg ctcgcatcgt ccgcggtaga gcctcaaatc cctcgcgcgg
     3421 ctcccgtgcc gcgagggctc gacgtagatc cgttggccca aacggcggcc gccatcgacg
     3481 cgctcgccat caccgcgcca gtcgcgcttc cgccaaccgt cgttggaccg gccgaagtgc
     3541 cccccgccgc ggctccgccc gaagcgcctc cggctccggt cgccaagcgg cgcctgactc
     3601 ccgcaaagct catcgagtct tttcctcggc tcgaggccgc cgcgcgcctc agtgaacacc
     3661 cgcggtttcg cgtgaaaggc atcagcccac cgatcgcggc gttgctcgtc gcaatcgcgg
     3721 tagtccaggg gctgctcgcc gctgtcattt acaagtacgt ccatcggacc gctcccgtcg
     3781 acgcgcgtga cctggcgagc gcggttgccc aaagcgcagc tactgcagcc gcacccgcgg
     3841 tgccggcaac gcctgctccc ggcgcgaccc cggccccgag cgcggccgcg acgccgttgg
     3901 catcgtccgc tccgaccccg agcgccagca cggagttgcc gcgcgacgag caacccgcgc
     3961 ccgcgccgag ctgcgacgag ctcctgaagg actcgccggg tggaggcgaa aatcccggcg
     4021 ccgccttcga gcagatcaaa cttgcgcgca aagcgctcgt acagggcaag tcagacgacg
     4081 cggagcgcgc ctactgcaaa gcggtacgct gggatcccca gaacatcggg tactacttcg
     4141 agctcgcgca gctgctcttg attcgtcggg acggagcggc cgccgccgag tgggcgcaaa
     4201 aaggcgcgcg cctcgacccc gcgaatacgc gcggccagtc gctggtcgga gacgggctcg
     4261 cgcgaatcgg cgacttcgac ggagcgcggc gagcctggta cgccgccgcc tccgtgaaca
     4321 cgccgagcgc ccaagaagtc gaacagctct tcttgcgcgc ccagaaagaa gccgagcaag
     4381 cgctcgccac ccacgactac atgcgggccg aacgcttttt ccggcgcgcg gccgtgctgg
     4441 acccgaaaaa tgcctctgcc cagcgcggct tggcctcgtc actgcttcga atcggcgaag
     4501 ccaagaacgc gctcgcttgg gcgcgccgag ccgaacagct cgcgccaaag gacgccactg
     4561 tccagctcac gctgggcgac gccctgctcg cgaccgggga cgacgccgga gctcgccaag
     4621 cgtggacgga ggcggaacgc ctcggttacc cggacgcccg ccgtcgcctg gaacgcctcg
     4681 cgaaaaggcc gtaggcttgc ggcgtgacag ctgaggcaga aacgtcgctc tttgccgagc
     4741 tcggcggaga gcccgcgctc cggcgcatca tcgatacctt cgtcgatcgc gtgttcgacg
     4801 acatcatgat cggatttctc tttcaacgcg cgaaccgaga gcgcatcaaa gagaaagagt
     4861 tcgaattcgc ggcggagttt ctcggtgctc ccgtcgagta cagcggacgc tcactcgagg
     4921 acgcgcaccg ccggcaccgc atcttcgacg gccagtttca gcgccgtctg accatcttgc
     4981 gcaacactct cgatgagctc agcgcaccgg cccgggtccg cgacgccctg ctcgcgcata
     5041 ccgtttcctt gcgtgctcgc gtcgtcgcgg gggattgtga cgagccggtc gctgcaatcc
     5101 ccggcagtcg tcccgaatga gccttcgccc agagaatgac taagccaacc actgccggtc
     5161 gctcctccaa gtccgagctc agcccacgcg tgggccggtg gcgctctcac gcgcgcctgg
     5221 gctctcgagc cgcgtggaag gcagtggggc ccctcgcagc gctctcggtc gccacggcct
     5281 gcgccaccgc cggctcctcc tggatggctc agcccctccc caacgagtcc tgggagggcg
     5341 aggctactga acccgatgct ccgcaggcat cccaaagctc gtcaccggag gctcgccccc
     5401 gcgcgcgaat catcggcgaa cgcgaacgca ccgaagcgaa cccggaggtc gcggaggcag
     5461 gcgccgcgac ccctcggctc gcattacaag gcggcggccg ctcgctcggc acctttcgaa
     5521 atacctacta cgacttcccg agcgaagccg acttcgacgg cgccaaggtc cccatcaaga
     5581 gcgctcgctg cgaaacgctc gccgaagtgc ctcgcggctt cttcgagagc gtctgcgttc
     5641 agggcagcgg cacgctacgc tcgggcgcga cggtcagctt tgccaaacgg gactgcgctt
     5701 gcgccgaaat ctgcccgcgc actgaccaaa agatctgctt cgactcactc gaccccgcgg
     5761 cttacccatg gggccggggc gcgacgggca aggccatcac gccccttctc accgtagcgg
     5821 tggacaccga cgtcattccg cttgggaccg ccctgtacgt cccggagctc gacggcatcc
     5881 ctcgcgatga cgcaggaacc acgcacgacg gttgcttcat cgcccaggat cgcggcgtac
     5941 gcgtcaaagg gaagcatgtc gactttttca ccggacatgc ttccctgacc gcgctttgga
     6001 accgcctggt cccgagcaac aaaggggtta ccgtcgtgct cgagtcgccg cgctgcgcac
     6061 gcgcggctcg ctaggcatca gccgccgacg ccgccgttgc ctgcagcagc accgccgttg
     6121 ccgccgttcg cagcgccacc ggcgccgcca ccagcagccg ccgcgccgcc attgcctgcg
     6181 gacgcaccgc cgtttccacc ggcgccgccg ccaccagcgc caccggcgct tgcagcagcc
     6241 ctctcctcgg gagtcatgat cttcacgtag acggcggtcg cgccgttctc gcgcacgtcg
     6301 aagtcgacgg ccgggggatg gtcaaaggac ccggtcgcgg caaaggcttc gacatccgcc
     6361 atcgccttgc catcggagaa cccgtcgagc ttgatgcgct cgaagaaatt ctcggcggtg
     6421 aaggtcatct tcgcgtcccc ttcgccttcc gtacgcgtcg gaagcatgtc gtagtcttta
     6481 gcgacttctt tgcctccctg acggatgctg acgtggatct tggtcagctg cggggccttc
     6541 ttcgaaacgt tcacgttcag ggccgcgatc gtgtccatgt ccgcgcaggc aagcgcggcc
     6601 acggcggtga gaaggcaagc tgccgcggca cggaacgact ttttgcttcg catgggggcg
     6661 gtcatttcac atcaactatc ctcgcgcgct ctagctatcg gattcgtgat tggctcggtt
     6721 cggcgcactc gaccaaccat cacgcttggc gagcacggct cgcgcgctca ggccaagaaa
     6781 aagtttcagg aactaagaag agacggccga ggtcctgggt ccagctaagg gcgacaggac
     6841 ctcgacttga cggtcaaccg aacctcacat gaatacggtg aggcggagac ccatctggcg
     6901 gggcgggttg tagaaacgca ggttcaggcc aggagtgttg atgagagagg tcatgtacgc
     6961 cttgtcggtc acgttgttga tgaaaccttc gaagcgcacc ttgccgctca cgtgcgtgaa
     7021 gccggcaccc acgtcgaagc gggtgaagcc cttgaccacg tcgctggtgt tcggatcgaa
     7081 gttgcccttc gaatcgaaac cgtcaccgtt gaacggagtc atgtagtact tcgtcttggt
     7141 ctggaaattg acgacccagt cgaagtagcc gatgctggtc tggatgttct gagtgatgcc
     7201 gtagttgacc gacagctgcg gggcgcgggg caggaacttg tccttcaagt tcacgtgacc
     7261 ttgcgtcgcg gggtcgtagc tgatgcgcgt gtcggcgatg tcgccttccg tgaagcgggc
     7321 atccatcagg aggccggaga gacggaggct gaagccggcc gggagacggc cgacgccttc
     7381 gagctcgagg cccatgacgc gcgattgagc ggcgttggag cgcaccgcgg aagccggagg
     7441 cgtccgggtg ggatccatcg gatcgccgat cgcgaccacc tgctgcagct gtaggtcttg
     7501 gtagctgtag tagaaggccg atgcgttcgc gatgacggct ttgttgagga gctcgttctt
     7561 gctaccgacc tcgatcgcgt acacgacctc gggcttgtac ttcggagcga gcgtcgcgcc
     7621 gctggggaac gtgacgttgt cgttgaagcc gccggacttg tggcccgtcg agaacagcgc
     7681 gtagagcagg ttgctcttcg tcaagttgcc gtcgacgccc acgcggaagt cgaagaactt
     7741 gtcgctgtag ctaccgtgct gctcgttggc gctacccgag aagccgacgc cctgcgcttg
     7801 cagcgccttg tccagcgtgt cgcgctgacc gaagcgcgcg atgccgttcc ggaacacgtc
     7861 gaagggcgaa gccttcgatg cgtccgtgat cgtgtagagc gtgcggccct ggtcggcgta
     7921 ggcgaagccg tccgtaccga agcgctgcga ttcgctgatg ccgctgaacg agtagacgtt
     7981 accgataccg tcgcgggact tctcttcagt cgtgccgcgg acgccggcgg tgacgcgcaa
     8041 ctccttcaag atgtcgaagg tgccgtcggc aaagcctgcc cacgaacgcg agtgcatctt
     8101 gggcatcgtg aactcgacgc cgaagaagcc gttcgacttg tcgttcgtcg tcgcgaagtg
     8161 cgtcttctgc tcttcgttga agaagaagcc gccgacgctc caacggaaac gcgcgctgtc
     8221 cggcgcgtag agacggagct cttgcgtgac cgacttcgag gtctggtgcc agtacgtcgt
     8281 gcccgagttg tcgacgtcgt agtcggtgcg acccgggaag tcgacgccag cctgaccgct
     8341 gttgatctgg ttgtagtcga ggtagcggta gctgccgacg tagccgatgt tgaggacgcc
     8401 gaggtcgacg ccgatgtcac cggtgatacc ccagtgcttc gagtcttcgc ggccttgcgg
     8461 agtgcggtag acgacgctgc ggacgtccgg aatctcttcc ggcgtgaggc ccgcggtgag
     8521 cgcgccgttc acgttgctgc cggtcgagcc ggtgccgcct tcctgcatgt aatcgtgctt
     8581 gacgatgatc ttgagggcgt cgaccgggtt ccacgacaga gccgcgcggt acgcgagggt
     8641 gtcggcagac tccgagcctt gcagccactt caccgggccg gcgttcttgt agaacggcga
     8701 gcgcgtctca cccgacgccg ccaagcgcaa ggccaagcgg tcgccgatcg gaatgttcaa
     8761 catgccgcgc acttcgcgct gcgagaagtt gcccaagccg accgatgcgt tcgcgtccca
     8821 ctccttgtac tggggcttcg cggtgacgat gttcaaggag ccggcggtcg cgttacgacc
     8881 gcggagcgta ccttgcgggc cgcggttgat ttcgacgcgc tcgaggtcga agaacatcgc
     8941 gccgacgccg cgaggacgcg ggatgtacac gccgtcgatg tgcgtcgcgg tcgcggggtc
     9001 accgagctcg gtgttgttgt tcgagccgat gccgcggatg taaacctcca tgttgttttc
     9061 ctgctggccg at
//
LOCUS       DASTKS010001135         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_180557, whole genome shotgun sequence.
ACCESSION   DASTKS010001135 DASTKS010000000
VERSION     DASTKS010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180557"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFQ35_24090"
     CDS             complement(<1..354)
                     /locus_tag="VFQ35_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933815.1"
                     /translation="MRRSGSSLQAAALCWGAAVLSGCSRAAPPSRFPDADTALARMRD
                     THSCSRGVSGEAKLDFYGEQGRVRGNLLYIVSAPDRVRLDVFSPFGATISTLTSDGRA
                     FALYDLRQKSFLRGPA"
     gene            complement(351..2174)
                     /locus_tag="VFQ35_24095"
     CDS             complement(351..2174)
                     /locus_tag="VFQ35_24095"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET9933816.1"
                     /translation="MTFTEAAAQVLRLVGKPLHYKEITDVAIEKNLLSHVGKSPEVTM
                     GARLAALVKKGDKENPLVRVKPGVFALREWDQETIEKGLKDRTPALEKLSGQDFEFPA
                     ESEGSGAVHVSLDDFAEEGEPRQPDAGERARAELAAHATDLFESEDDDDRPIFSDDEE
                     PEAGDAQSKDAAGGRRRRRRRRRGRGADERGGDELPSYTVSDAPADLPEVPIEAEARE
                     GREPREVPRENGRAERDNLREPREAREPREGREPREPREARESREGREPREAREPREG
                     REPREPREARESREGREPREAREPREGREPRESREFNGREFREGRDRDREGRDRADRE
                     VRDDRSSPLNELAGRSLADAVESLLSGFDRSRGPAPLQALADAAQRRGRLNGEQGVGL
                     SLIYAAVRADNARRTAEGRRPRFRLSGNKIALSDWQNEGELGRAERDLFNALERYREA
                     ARRSLLRSLQELPHRALGELVVLLLEQMGYSELSPVRRPGAHGAELHLTGKARGNLGE
                     MRTAIVVRRDGREIGRERVTELRGGLHHYGAATAGVLITTGQALSGAREEASAPGAAP
                     VAVIDGVALARLCDEHRVGVCELSLRLPMPDTELLDGLRSG"
     gene            2460..2816
                     /locus_tag="VFQ35_24100"
     CDS             2460..2816
                     /locus_tag="VFQ35_24100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933817.1"
                     /translation="MAPAELLTELLRVAETIGLEVRCFGLRRKNAGAGGLCTLNGRPV
                     VILNLQTSAIDRGTALADALAGRDLSEVPMPSRVRAFIAARTRARSRLLAPFRGPGPG
                     LARCPNDLGRRQSGKT"
     gene            2827..>3339
                     /locus_tag="VFQ35_24105"
     CDS             2827..>3339
                     /locus_tag="VFQ35_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9933818.1"
                     /translation="MEILRDVPLAQRTSLRIGGSARFFVEAANEAEVAEACEWARERG
                     ARFWVLGGGSNVVVPDTGLDGVVLALGLRGVTLEDAGSGVRLRAGAGESWDTVVARSV
                     EAGLWGIECLSGIPGQVGATPIQNVGAYGQEVAETLELVRAFDRERRSFVEIAARECA
                     FSYRDSVFKSR"
BASE COUNT          497 a         1166 c         1106 g          570 t
ORIGIN      
        1 cgcgggcccc cgcaaaaagc tcttctgacg gagatcgtag agcgcgaagg cgcggccgtc
       61 ggaggtgagc gtcgatatgg tggcaccgaa agggctgaac acgtcgagcc gcacgcggtc
      121 cggagccgac acgatgtaga gcaagttgcc ccgcacgcgc ccctgctcac catagaagtc
      181 gagcttggcc tcgccgctca cgccgcgact acacgagtgc gtgtcgcgca tgcgcgcgag
      241 cgcggtgtct gcgtcgggaa atcgcgaagg cggtgctgcc cgagagcaac cggacagcac
      301 ggcggcgccc caacagagcg ccgccgcctg caacgacgaa ccgctgcgtc tcatcccgag
      361 cgcagcccgt cgagcagctc cgtgtccggc atcggcaagc gcagcgagag ctcgcacacg
      421 ccaacgcgat gctcgtcgca caaccgcgcg agggcgactc cgtcgatgac cgcgaccggc
      481 gccgcgccgg gtgcagacgc ctcttcgcgc gctcccgaga gcgcttgccc agtcgtgatg
      541 agcacgcccg cggttgcagc gccgtaatgg tgcagcccgc cgcggagctc ggtgacgcgc
      601 tcgcgaccga tctctcgacc atcgcgtcgc acgacgatgg cggtgcgcat ctcgccgagg
      661 ttgccgcgag ccttgccagt gaggtgcagc tcggctccgt gtgcgcccgg ccgacgaacc
      721 ggcgagagct cggagtagcc catttgctcg agcagcaaga ccacgagttc gccgagcgcg
      781 cgatgcggta gctcttgcaa gcttcggagg aggctccgcc gagccgcttc acggtagcgt
      841 tcgagcgcgt tgaacagatc gcgctcggcg cgcccgagct caccctcgtt ttgccaatcg
      901 ctgagcgcaa tcttgtttcc gctcagtcga aaccgaggac ggcgcccctc ggccgttcgg
      961 cgcgcgttgt cggcgcgaac ggcagcgtaa atcagcgaga ggccaacgcc ctgctcgccg
     1021 ttcaagcgac cacggcgctg cgcggcatcg gcgagcgcct ggagcggcgc ggggccgcgg
     1081 ctccggtcga aaccggagag cagcgactcg acggcgtcgg ccagagagcg cccggcgagc
     1141 tcgttgagcg gagaagaacg atcgtcgcgc acttctcggt ctgcgcggtc gcgtccctcc
     1201 cgatcccgat cgcgaccttc gcgaaactca cgaccgttga actcacggct ttcgcgcggc
     1261 tcgcggcctt cgcgcggctc gcgagcttca cgcggctcgc ggccttcgcg cgactcccga
     1321 gcttcacgcg gttcacgcgg ttcacggcct tcgcgcggct cgcgagcttc acgcggttca
     1381 cggccttcgc gcgactcgcg cgcttcacgc ggttcacgcg gttcacgacc ttcgcgcggc
     1441 tcgcgagctt cacgcggctc ccgcaagttg tcgcgctcgg cgcgcccgtt ctcgcgtggg
     1501 acttcgcgcg gctctcggcc ctcacgagcc tctgcctcga ttggcacttc gggcagatcc
     1561 gccggggcgt cgctgaccgt atacgacggc agctcatcgc cgccccgctc gtcggcacct
     1621 cgaccccgcc gccgacgacg gcgacgacga cgaccaccag ccgcgtcctt cgactgcgcg
     1681 tcacccgcct cgggctcctc gtcgtcgctg aagatcggac gatcgtcatc gtcctctgac
     1741 tcgaaaagat ccgtcgcgtg cgcggcaagc tcggcgcgcg ctcgttcgcc cgcgtcgggc
     1801 tgtcgcggct caccctcttc tgcaaagtcg tcgaggctca cgtggacggc cccgctgcct
     1861 tcggactcgg cggggaactc gaaatcttgc ccggacagct tctcgagcgc cggagtgcga
     1921 tccttgagcc ccttttcgat cgtttcctga tcccactcgc ggagggcgaa aaccccgggc
     1981 ttcactcgca ccagcgggtt ttccttgtcg cccttcttga cgagggcggc gaggcgcgca
     2041 cccatcgtga cttccgggct cttgccgacg tgactgagga ggttcttctc gatcgctacg
     2101 tccgttattt ccttatagtg taggggcttg cccacgaggc gcagaacctg cgccgcagct
     2161 tccgtaaacg tcatcagaga gtgtctttcg cttggaagag aacatcgttc tctcggcctt
     2221 cgaggcgcct ccggggaagc gcgaatcgct cccgcctccg gcaggacgcc tctcactagg
     2281 ccacctcaat agcacgcccg ccgtcatatg gcgcgaaatc ggctgttctc gaggggtcga
     2341 actcggtccc cctgccccgc cctcaaactg tgagaattcc gctgaattcg tgctggttcc
     2401 gcccgtcgcc aaccgccgcg tgctcgcttc cggcgggccg ctgagggtat tatggcgcgg
     2461 tggcccccgc tgaattgctc accgagctct tacgcgtggc ggagaccatc gggctcgagg
     2521 tccggtgctt cgggctgcgg cgaaagaacg ccggggccgg cgggctctgc accctcaatg
     2581 ggcgaccggt cgtgattctc aatctgcaaa ccagcgcgat cgaccgaggc accgctctcg
     2641 ccgatgctct cgctggccgc gacttatccg aagtcccgat gcccagccgg gttcgcgcgt
     2701 tcatcgccgc tcgcacgcga gctcgttcgc gacttctcgc gccgtttcgc gggccgggtc
     2761 cagggctcgc acggtgcccc aacgacctcg gacgccggca gagcggaaag acctgaggac
     2821 gatccgttgg agattctgag agacgttcca ctcgcccaac gcaccagctt gcgcatcggt
     2881 ggttcggcgc ggttcttcgt cgaggcggca aacgaggcgg aagttgccga ggcttgcgaa
     2941 tgggcgcgcg aacgcggcgc tcgtttctgg gtgctcggcg ggggcagcaa tgtcgtagtc
     3001 ccggataccg ggctcgacgg agtggtcttg gcgctcggct tgcgcggcgt cacgctggag
     3061 gacgccggca gcggagtgcg gctccgcgcg ggtgcgggcg agtcttggga caccgtcgtc
     3121 gcgcggagcg tcgaggcggg actgtggggt atcgaatgcc tcagcggcat ccccggccaa
     3181 gtcggcgcga ccccgattca aaatgtgggc gcttacgggc aagaggtggc ggagacgctc
     3241 gagctcgtgc gcgctttcga tcgcgagcgg cgctcgttcg tcgaaatcgc ggcgcgcgag
     3301 tgcgcctttt cttatcgcga cagcgtcttc aagtcgcgt
//
LOCUS       DASTKS010001136         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1522152, whole genome shotgun sequence.
ACCESSION   DASTKS010001136 DASTKS010000000
VERSION     DASTKS010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522152"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VFQ35_24110"
     CDS             complement(<1..1630)
                     /locus_tag="VFQ35_24110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9933819.1"
                     /translation="MTNPEHARLLAAWSAAWPRALEAWSAYTLLREPRFFESNEAAQS
                     DGMAGEFAAIRLRDHSVMVNVAEVLKRGLEPHALPILAHEIGHHVYVPGNLTDNARML
                     AAIARMLTGLPKGSVHLVANLYGDLLINDRLHHRADIDVASVYVQLKQSAGERAPNQV
                     WSVYTRTYEHLWRLKAGTLAPERVGYEMDADAMLLARLVRSFAGDWLRGARRVATVLY
                     RYLAQDELDNAGKTFVELGLHDTGRAGAAAPGEGEADAAPDGLAAVDASEIGDDDEFE
                     DELADPLGKGRQSAVPEAPNTAPGKEGQGHSGTQYRTPFEYGELLRTLGLNLDAHEIT
                     TRYYRERALPHLIPFPVRRAPQATEPLAEGYEEWNPGEALEALDLFGSILRSPRVIPG
                     VTTVQRTYADVPGNDPAKVPMDLDIYVDCSGSMPNPAFDVSYLSLCAAILALSALRAG
                     ARVQATLWSSAGTFETTGGFLRDERRVLGTVTGYVSGGTAFPLHLLRDTYQERKASEP
                     PAHIVVISDDGCDTMLAKDEFGNDGASVAATALAR"
     gene            complement(1627..2745)
                     /locus_tag="VFQ35_24115"
     CDS             complement(1627..2745)
                     /locus_tag="VFQ35_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933820.1"
                     /translation="MDRVITGYAADWVGARRDDLNRRFQRAQQRVPTLSPGAVLDTLA
                     DVLPALAGPEPAAGELLSSVYDLVILHAARDTFATRPGLDCLLREVFPRIRPLLLTRP
                     LQLPGALSNAVENLGKRGREFALELGRVLAGVERAEVAFDLAGVVAWCLGEARLRDSA
                     LEAAARLPGGSFLRALGLDDFPETSAPLVLAGLRAHGFQHPRTLWREETLRASDHAAL
                     LARLQAKQAVPLANYRLLMHVGDFVGFGGHFQVPPWVLDGGGEHHFFARALSQDFRID
                     ADVFGWSCTPVQEVGLSLRPVGGGTSRLRQWLGSFSSSGADAPRLEAAGTLTLRGDRA
                     NLPALARATAFTVLPGAVVSSHADSHRLRVVGPLRDAL"
     gene            complement(2733..4334)
                     /locus_tag="VFQ35_24120"
     CDS             complement(2733..4334)
                     /locus_tag="VFQ35_24120"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933821.1"
                     /translation="MSAPRPPRDPQQVSASPTLPASATERGRKFDVKSLFAGPAATGD
                     AEGASLGARALDDKMRQAYYWIVNHAIITPYYDIEFHETAPSSFRFGGAGTEVRLPTA
                     PSYSSFVLLPLLTFASRKRCLFIGGPGRGKTASALLMGVLAGYSTKEVKRAIQHGQPQ
                     MTIADLIGNPLPKDLVEAASMENIRIAWRKWLSMRVKIVDEYNRIPTRTQSALLTLLG
                     DGYAEILDQTMDSPESAWFLTANDDMGGGTYQVIEALKDRIDIVVKALNFNPRFLSEL
                     LMRVEERVKPEEIVPREIIFSEAELDEIYQQVLRIPLPRPVRRRLEFFASQFDFCDLA
                     AQDLEYKTKDTIRIAGKSLSTVCANDCGRDKVRSICSQTENGLSVRSLLTLLSFVKTL
                     AWFRGAPEVSLEDVRQLVPWVLHEKLVQNPTSPFFDQSTNGIYRIDRVAWIRRAFDLA
                     CEEYVRLDLDRSDPIMAIDDVFDRGLDGVPEPEVRKRMHEIETHLAQLAKGTKLYAHV
                     QNDVLKLKYYHQRYSNYLRWLTWTG"
     gene            4486..>5441
                     /locus_tag="VFQ35_24125"
     CDS             4486..>5441
                     /locus_tag="VFQ35_24125"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET9933822.1"
                     /translation="MKSLMIQGTSSDAGKTVLVAGLCRSFANRGLRVAPFKSQNMALN
                     SYVTPHGEVVARATALQARAARQELCVHMNPLLLKPKGDELAQLMVHGKPCMDVTASQ
                     YFLSDELQALKLRAIDESIAALRERYDVVIAEGAGSSAEPNLRRLDVVNMGVAHRLGA
                     RVWIAVDIDRGGAFASMLGTLEVLRATSPADLDLVDGFVLNKFRGDRRVLEPALDFMK
                     ERSNVPILGVLPYLPDLNLDEEDRLRERRCDNPEVDIAVIYLPHISNATDFRFFEAEH
                     NVQVRYVRSAAALGRPDAIIIPGTKNTTWTSTTSAASGSSAPS"
BASE COUNT          979 a         1781 c         1770 g          911 t
ORIGIN      
        1 cccgagcgag agcggtcgca gcgacgctgg cgccgtcgtt accgaactca tccttggcga
       61 gcatggtgtc gcagccgtcg tcggagatga cgacgatgtg ggcggggggc tcgctcgctt
      121 ttcgctcttg gtaggtgtcg cgcaagaggt ggagggggaa cgcggtgccg ccgctgacgt
      181 agccggtgac ggtgccgagc acgcggcgct catcacgcag aaagccgcct gtggtctcga
      241 acgtgccggc cgacgaccac agcgtggcct ggacgcgagc accggcgcgc aaggcgctga
      301 gcgccaagat ggcggcgcag agagaaaggt agctcacgtc gaacgcgggg tttggcatgc
      361 tgccggaaca atcgacgtag atgtcgaggt ccatcgggac cttggcgggg tcgttgcccg
      421 gcacgtccgc gtaggtgcgc tgcacggtgg tcacgcccgg aatcacgcgc ggcgagcgca
      481 agatcgagcc gaacagatcg agtgcctcga gcgcttcgcc ggggttccac tcttcgtagc
      541 cttcggcgag gggctcagtc gcttggggcg cgcggcgtac aggaaaggga atcaggtggg
      601 gcagcgcgcg ttcgcggtaa tagcgcgtgg tgatttcgtg tgcgtcgagg ttcaaaccca
      661 gcgtgcgcaa gagctcgccg tattcgaacg gcgtgcggta ttgcgtgccc gaatggcctt
      721 gaccctcctt gccgggcgcg gtgttcggcg cctcgggcac cgcactctgt cgccctttgc
      781 cgaggggatc ggcgagctcg tcttcgaact cgtcgtcgtc gccgatctcg ctcgcgtcga
      841 cggcggcgag cccatcggga gcggcgtcgg cctccccttc ccccggcgca gcggcccccg
      901 ctcgaccggt gtcgtgcaag ccgagctcga cgaaggtctt gcctgcgttg tcgagctcat
      961 cttgggcgag gtagcgatag agcaccgtcg ccacacgacg cgcgccgcgc agccaatcgc
     1021 cggcaaagct gcgcacgagg cgcgccagca gcatcgcgtc cgcgtccatc tcgtagccga
     1081 cgcgctcggg agccaacgtg ccggccttca aacgccacag atgctcgtag gtgcgcgtgt
     1141 acaccgacca cacctggttc ggcgcgcgct cacccgcgct ttgcttgagc tgcacgtaca
     1201 ccgaagccac atcgatgtcg gcgcggtggt gtaaccggtc gttgatcagc aagtctccgt
     1261 agaggttggc cacgaggtgg acgctgcctt tgggcagccc ggtcagcatg cgcgcgatcg
     1321 ccgcgagcat gcgggcgttg tcggtcaggt tgccgggcac gtagacgtga tgcccgattt
     1381 cgtgcgccaa aatcggcaag gcgtgcggtt ccaagccgcg cttgagtacc tcggccacgt
     1441 tcaccatcac gctgtggtcg cgcagacgaa tggccgcgaa ttctccggcc atgccgtcag
     1501 actgcgcggc ctcgttgctc tcgaaaaagc gcggttcgcg cagcaaagtg tatgccgacc
     1561 aggcctcgag cgcgcgcggc cacgccgcgc tccacgccgc cagcagccgt gcgtgctccg
     1621 ggttggtcat agcgcgtctc gcagcggccc caccactcgc aagcggtgcg agtccgcgtg
     1681 cgagctgacg acggcccccg gcaggacggt gaaggccgtg gcgcgcgcca gcgcaggcaa
     1741 attcgctctg tcgccgcgca gcgtgagagt gcccgctgct tccaatcgcg gcgcgtccgc
     1801 gccggaagac gaaaacgagc cgagccactg gcgcaaccgc gaagtgccac cacccactgg
     1861 ccgcagcgaa agcccgacct cctgcaccgg cgtgcaactc cagccgaaca cgtccgcgtc
     1921 gatgcgaaag tcttgcgaga gcgcgcgagc gaaaaagtga tgctcaccgc cgccgtccaa
     1981 gacccagggc ggaacttgaa aatggccacc aaagccgacg aagtcgccca cgtgcatgag
     2041 caaacggtaa ttcgcgagcg gtacggcttg ctttgcttgc aagcgagcga gcagagccgc
     2101 gtgatcggac gcccgcagag tctcctctcg ccacaaggtg cgcggatgct gaaacccatg
     2161 ggcgcgtaag ccggcgagca ccaggggcgc gctcgtttcc gggaaatcgt cgagccccag
     2221 cgctctcagg aacgagcctc ccgggagccg tgcggccgct tccagcgccg aatcccgcag
     2281 tcgcgcctcc cccaagcacc acgccaccac gccagccagg tcgaacgcca cctcggctcg
     2341 ctcgacccca gccaacacgc gcccaagctc gagagcaaac tctcgcccgc gcttccccaa
     2401 attctcgacc gcgttggaga gcgcgccggg aagctgcaga gggcgagtca acaacagcgg
     2461 ccggatccga ggaaacacct cgcgcagcaa acaatcgaga ccgggtcggg tcgcgaaggt
     2521 gtcgcgcgcc gcgtgcagaa tcaccaaatc gtacaccgac gagagcagct cgcccgccgc
     2581 cggttcgggg ccggccaacg ctggcagcac gtccgccaag gtgtcgagca ccgcccccgg
     2641 cgacagcgtg ggaacacgct gctgggcgcg ctgaaagcga cggttgaggt cgtcgcgccg
     2701 cgcgccgacc cagtctgcgg cgtaacccgt aatcacccgg tccatgtgag ccagcgcagg
     2761 taattcgagt agcgttggtg gtagtacttg agcttgagca cgtcgttttg cacgtgcgca
     2821 tagagcttgg tgcctttggc cagctgcgct aggtgcgtct cgatttcgtg catgcgcttg
     2881 cgcacttctg gctcaggcac tccgtcgagc ccgcggtcga acacgtcgtc gatcgccatg
     2941 atcggatcgc tacgatcgag atcgagccgc acgtattctt cacaggcgag gtcgaaagcg
     3001 cgacgaatcc acgcgacgcg atcgatccgg taaatgccgt tcgtgctctg gtcgaagaac
     3061 ggcgaggtcg ggttttggac cagcttttcg tggagcaccc agggcaccaa ctgccgcacg
     3121 tcttcgagcg aaacttcggg cgcgccgcgg aaccaggcca gtgtcttcac gaagctcagc
     3181 aacgtgagca gcgagcgcac cgacagccca ttctcggttt ggctgcaaat cgagcgaacc
     3241 ttgtcgcgcc cgcagtcgtt cgcgcacacg gtggagaggg attttccggc gatgcgaatc
     3301 gtgtctttgg tcttgtattc gagatcttgc gcagccaaat cacagaagtc gaactgcgac
     3361 gcaaagaact cgagccgtcg ccgcaccggg cgcggcagcg gaatccgcaa gacctgttgg
     3421 tagatctcgt cgagctcggc ctcgctgaag atgatctcgc gcggcacgat ctcttctggt
     3481 ttgacgcgct cttcgacgcg catcaggagc tcggacagaa agcgtgggtt gaagttcagg
     3541 gccttcacca cgatgtcgat gcgatctttc agcgcctcga tgacttggta ggtgccgcca
     3601 cccatgtcgt cgttggcggt gagaaaccac gcgctctccg ggctatccat ggtttgatcg
     3661 aggatctccg cgtagccgtc acccagcaag gtcaacagcg ccgactgcgt gcgcgtcggg
     3721 atgcggttgt actcgtcgac gattttgacc cgcatcgaaa gccacttgcg ccaggcgatg
     3781 cggatgttct ccatgctggc cgcctcgacc aagtctttgg gcaacggatt gccgatcaga
     3841 tcggcgatgg tcatctgcgg ttggccgtgc tggatcgcgc gcttcacctc tttggtggag
     3901 tagcccgcca gcacccccat caggagcgcg ctggccgtct tgccacgtcc cggcccgccg
     3961 atgaacaaac agcgctttct gctggcaaac gtcaacagcg gcaacagcac gaaggacgag
     4021 tacgacggcg cggtcggcag gcgcacttcc gtacccgctc ccccaaatcg aaacgacgac
     4081 ggcgccgttt cgtgaaactc gatgtcgtaa tacggcgtga tgatcgcgtg attgacgatc
     4141 cagtagtaag cctgccgcat cttgtcgtcg agagcgcgcg ctccgagcga cgcgccttca
     4201 gcgtcgcccg tggcggcggg cccggcgaac aggctcttga cgtcgaactt gcggccgcgc
     4261 tcggtcgcgc tcgcggggag cgtcggtgaa gcgctgacct gttgcggatc acggggtggt
     4321 cgaggtgctg acaagggtcg cgatagttcc ccgcgtagta gaaagcgtca agcagccgct
     4381 ggggcgagtg cgagcagcag gaaccgcacc gcgcgggctg gtgtgcgccc agaaaaaatg
     4441 gcttaaggag cgctgctcga caaatcggtc ggagtccgcg catcgatgaa atccctcatg
     4501 attcaaggca cgagctccga tgccggcaag accgtcctcg tggcgggcct gtgtcgctcg
     4561 ttcgcgaacc gcggccttcg cgtcgccccg ttcaaatcgc agaacatggc gctgaactcg
     4621 tacgtcacgc cgcacggcga agtggtggcg cgcgccaccg cgttgcaggc ccgcgccgcg
     4681 cggcaggagc tctgcgttca catgaatccg ctcctcttga agcccaaggg cgacgagctg
     4741 gcccagctga tggtgcacgg caaaccgtgc atggacgtca cggcttcgca gtatttcctc
     4801 agcgatgaac tgcaagcgct aaagctccgt gccatcgacg agtccatcgc agcactgcga
     4861 gagcgctacg acgtcgtgat cgccgaaggc gcgggcagct cggcggagcc caacttgcgg
     4921 cgcctcgacg tcgtcaacat gggcgtcgca catcggttgg gagcacgcgt gtggatcgcc
     4981 gtggacatcg accgaggcgg cgcctttgcg agcatgctgg gcacgctcga ggtactgcgt
     5041 gcaacgtcgc cggctgacct cgacctcgtc gacggcttcg tgctcaacaa gttccgcggc
     5101 gaccgacgcg tgctggaacc tgcgctggac ttcatgaaag agcggagcaa cgtgcccatc
     5161 ctgggcgtgc tcccctacct gcccgaccta aatctcgacg aagaagaccg tctgcgcgag
     5221 cggcgctgcg acaacccaga agtcgacatc gccgtgatct acctgcccca catttcgaac
     5281 gcgacggact ttcgcttttt cgaggcggag cataacgttc aggttcgcta cgtgcgctcg
     5341 gctgccgccc tcgggcgacc cgatgccatc attattccgg gcaccaagaa caccacctgg
     5401 acctcgacta catccgccgc atcgggctcg agcgcgccat c
//
LOCUS       DASTKS010001137         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_180789, whole genome shotgun sequence.
ACCESSION   DASTKS010001137 DASTKS010000000
VERSION     DASTKS010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180789"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="VFQ35_24130"
     CDS             complement(<1..2267)
                     /locus_tag="VFQ35_24130"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9933823.1"
                     /translation="MSSANLASSGAGPGSWRYTPSAGFDEAFDPNGEVRPHFRELFDT
                     LQSLSKDELDRRWGQAQRAIRENGVTYNVYGDPRGTDRPWQLDPLPLVVSPDEWASLE
                     VGLMQRARLLDTVLWDLYGAQRVLREGLLPPELVLGNPAFLRPLFGVRVADSARLHFY
                     AADLSRGPDGQFWVFGDRTQAPSGAGYALENRIVISRLLPQAFRNCRVERLAPFFRAL
                     REGLQRLVRRRTDNPRIALLTPGPYNETYFEHAYLARYLGFTLVEGGDLTVRDRMLYL
                     KTLGGLERIDVLVRRLDDSFADPLSLRPDSSLGVAGLVHAVRAGNVTIANSLGSGLVE
                     MPALLAFLPALSRALLGEELLLPNVPTFWCGDEKSRAYVLEHLGELVVKSAMPGPNTK
                     EPVFGGDLSSGELYELRQKILARPFAYVGQERLPLSTAPAHTEEGIQPRHVSLRTFLA
                     RRDEGYMMMPGGLTRVASRKDSVVVTIQRGGGSKDAWVLARGEPSNFSLLPPPDRRVE
                     VVRSGGDLPSRVADNMFWIGRYIERAEGTTRLMRSLIARRIDDSGGRAPEVRALLGAL
                     EDNLELIRGALVRAVPGVEQELTRLLVSAEPGRTPGAGIEAGHRAARVVRDRISLDTW
                     RVLGQLAATIAQARVRGNLEPSDALETVSSLMLGFSAFSGLVMENMTHGPGWRFADIG
                     RRIERGSFMVRLVRSMVVGAEDSAVLDALLEVADSAMTYRSRYLGTMALEPVLDLVLT
                     DETNPRSIVYQLAVL"
     gene            complement(2282..>3096)
                     /locus_tag="VFQ35_24135"
     CDS             complement(2282..>3096)
                     /locus_tag="VFQ35_24135"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9933824.1"
                     /translation="MPPHSRMSLVQMLLVRSLVSRFWQAPYTPRLPRWGTSLHDRFLL
                     PHFTWQDFTDVLRDMSEAGYPFEDSWFRPHFEFRFPEVGKVTYDSVEIEVRRALEPWN
                     VLAEEASAAGVVRSVDSSLERVQVRLRGAVPGRHALVCNGARVPLHPTGTRGEAVAGV
                     RFRAWWLPSQLHPTQGVHAPLEFSLIDEWAGRALGGCMLRVSHPGGRSYDRFPVNALE
                     AEARRAALFSPFGHQPGQLDLSRIVDTRNPDYPLTLDLRRLRVARSGSGQMW"
BASE COUNT          568 a         1052 c         1021 g          455 t
ORIGIN      
        1 tcgagcaccg cgagctggta cacgatggag cgcgggttcg tctcgtcggt caaaacgaga
       61 tccaacaccg gctcgagcgc catcgtccca agatagcgag agcgataagt catcgcgctg
      121 tccgcaacct cgagcagagc gtcgagcacc gcgctgtcct cggcgccgac caccatgctg
      181 cgcacgagcc gcaccatgaa gctacctcgc tcgatgcgcc gaccgatatc ggcgaagcgc
      241 caaccagggc cgtgcgtcat attttccatg acgaggccag aaaaggcaga aaagccgagc
      301 atcagcgagc tgaccgtctc gagcgcgtcc gaaggctcga ggtttccccg gacgcgcgct
      361 tgcgcaatcg tggccgcgag ctggccgagc acccgccagg tgtcgagcga aatacgatcg
      421 cgcacgactc gcgccgcgcg gtgccccgcc tcgatgcctg cgcccggcgt tcgccccggc
      481 tccgccgaga ccaggagccg cgtgagctcc tgctcgacgc cgggcaccgc gcgcaccagc
      541 gccccgcgga tgagctcgag gttgtcttcg agcgcaccga gcaaggcccg cacctccggt
      601 gcccgcccgc ccgaatcatc gattcgtcgc gcgatgaggc ttcgcatcag gcgggtcgtg
      661 ccctcggcgc gctcgatgta gcgcccgatc cagaacatgt tgtcggcgac gcggctcggc
      721 aagtcgccac cgctccgcac gacctcgacc cgtcgatccg gcgggggcag taggctgaag
      781 ttgctcggct ctccgcgcgc gagaacccag gcgtctttgc tgccgccgcc acgctgaatc
      841 gtcaccacca cggagtcttt gcggctcgcg acgcgggtga gccctccggg catcatcatg
      901 tagccttcgt cgcggcgagc caagaaggtg cgcaacgaga cgtgccgcgg ctgaatacct
      961 tcctcggtgt gcgcgggcgc ggtcgacaga ggcagccgct cttgcccgac gtacgcgaat
     1021 ggacgcgcca gaattttctg ccggagctcg tagagttcgc ccgagctcag gtccccaccg
     1081 aagaccggct ccttggtgtt gggccccggc atcgcgctct tgacgacgag ctcgcccaag
     1141 tgctcgagca cgtaggctcg cgacttttcg tcgccacacc agaaggtggg cacgttgggc
     1201 aagagcagct cttcccccag gagggcgcgc gacaaggcgg gcaagaacgc gaggagcgcc
     1261 ggcatttcga cgaggccgct accgagcgag ttcgcgatgg tcacgttccc ggcgcgcacc
     1321 gcgtgaacca gccccgcgac gccgagcgat gaatcgggac gcaagctgag cgggtcggcg
     1381 aagctgtcgt ccaggcgccg cacgagtacg tcgatgcgct cgagaccgcc gagcgtcttc
     1441 aagtacagca tgcgatcgcg cacggtgaga tcgccgccct cgaccagcgt gaagccgagg
     1501 tagcgcgcga ggtacgcgtg ctcgaaatac gtctcgttgt aggggccggg cgtgagcaac
     1561 gcgatgcgcg ggttgtcggt gcgacggcgc accaagcgct gcaatccttc gcgtagggcg
     1621 cgaaagaacg gcgcgagccg ctcgactcga cagttccgaa acgcctgcgg caaaaggcgc
     1681 gagatgacga tgcgattctc gagtgcgtag cccgcgccgc tcggtgcctg cgtgcgatcc
     1741 ccgaagaccc agaactgacc gtcggggccg cgcgacaagt cggccgcgta aaagtgcaaa
     1801 cgagcgctgt cggctacgcg aaccccgaac agcgggcgca ggaacgccgg gttgccgagc
     1861 acgagctcgg gaggcaagag cccctcgcgc agtacgcgct gcgcgccgta gagatcccag
     1921 agcacggtgt cgagcaaccg cgctcgttgc atgagcccaa cttcgagcga tgcccactcg
     1981 tcgggcgaaa cgacgagcgg cagcggatcg agctgccatg ggcgatccgt cccgcgcgga
     2041 tcgccgtaca cgttgtaggt gacgccgttc tcgcggatcg ctcgctgcgc ctggccccaa
     2101 cggcggtcaa gctcgtcttt cgagagcgac tgcagcgtgt cgaagagctc tcgaaaatgc
     2161 ggccgcacct cgccattggg atcgaatgcc tcgtcgaagc cggctgaggg cgtatacctc
     2221 cagctccccg ggccagcgcc cgagctcgcg agattggcgg acgacattcg aacttacgtt
     2281 actaccacat ctgccccgag cccgaccgcg cgacccgcag gcgtctcaaa tcgagcgtga
     2341 gcggatagtc ggggtttctc gtgtccacga tacggctcaa atcgagctgt ccgggttggt
     2401 gtccaaacgg cgagaataac gccgcgcggc gcgcctcggc ttcgagcgcg ttcactggga
     2461 agcggtcgta actgcgaccg ccggggtgcg acacccgcaa catgcagcca ccgagcgccc
     2521 gccctgccca ctcgtcgatc agcgagaatt cgagcggagc gtggacgccc tgggttggat
     2581 gcagctgcga aggcagccac caagcgcgga agcgtactcc cgcgaccgct tcgccgcggg
     2641 tcccggtcgg gtgaagcggc acgcgagcgc cgttgcagac gagcgcgtgg cgccccggaa
     2701 ccgcgcctcg caagcgcacc tgcactcgct cgagcgacga atcgacgctg cgcaccacgc
     2761 ccgcggcgct cgcctcttct gcgagaacgt tccacggctc gagtgcgcgc cgaacctcga
     2821 tttccaccga gtcgtaggta accttgccga cctcggggaa gcgaaactcg aaatggggac
     2881 ggaaccagct gtcctcgaac gggtagcccg cctcgctcat gtcgcgcaaa acgtcggtga
     2941 agtcttgcca cgtgaagtgg ggtagcaaaa atctatcgtg aagcgacgta ccccagcgcg
     3001 gcaagcgagg cgtgtacggc gcttgccaga agcggctcac cagcgagcgc acgagcagca
     3061 tttgcacgag gctcatgcgc gagtgcggcg gcatct
//
LOCUS       DASTKS010001138         6693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_626732, whole genome shotgun sequence.
ACCESSION   DASTKS010001138 DASTKS010000000
VERSION     DASTKS010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626732"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFQ35_24140"
     CDS             complement(<1..932)
                     /locus_tag="VFQ35_24140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933825.1"
                     /translation="MLPALLLELTLLGAPARPPEVAALRAFAAASPRPAPCRPAPLLD
                     ENGLWERTRGGVTERYCGLLAFGYARLRDAPERALELAREAEKLNPNEPEARVLAGRA
                     ALRLGDFAGAYAALSGSVTDPRRPLGDVAALREFGIVAQVSGHLNEAAASYRALIPRV
                     AFTNDPVLVRLVTLEGASVLARSGPEGLSDAVLYLSEARRLTSAPGLDDLSTALLASC
                     LDRAGNGEQAQVLARALEGGFSLERFASPRDRERVAQAALGGAVSAARSQFEFSERAP
                     FLAEGELHAAIGYAALGRDARLARAHLQAYLSLGG"
     gene            complement(938..2251)
                     /locus_tag="VFQ35_24145"
     CDS             complement(938..2251)
                     /locus_tag="VFQ35_24145"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9933826.1"
                     /translation="MSAVVIVGAQWGDEGKGKIVDIYTEFADVVVRYAGGPNAGHTLV
                     VGDDKIVVRLLPSGILRPSTRCVLGQGMVVDPGVLLDEIDGLIRRGHADVEKRLVLSD
                     RAHLILPYHILVDGLREASADDRAIGTTKKGIGPAYEDKARRTGVRLGDLRDLGRVRT
                     RIEAAVDAWAPTIRALGGDLPNVDEIMGSLEKAAKRLVPLLGNTSLLVDSAIRRRERV
                     MLEGAQGTLLDLDHGTYPYVTSSSAVAGGAAIGTGIGPNRINTVLGITKAYTTRVGAG
                     PFPTELDDADGRHLREVGVEFGSVTGRPRRTGWLDLPGLRYAARVNGLDALALTKLDV
                     LTGMSRIKVCVAYDTPSGRVVDLPIDELHELGNTVKPVYEELEGWSERIENARVLDDL
                     PRAARAYVRFIEEQSQVPLYLVSVGPRRNETIVLHNPFVGRAAAP"
     gene            complement(2267..3493)
                     /gene="hisZ"
                     /locus_tag="VFQ35_24150"
     CDS             complement(2267..3493)
                     /gene="hisZ"
                     /locus_tag="VFQ35_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9933827.1"
                     /translation="MRDLLPPEAWRHADLARRILRAFELAGYELVVVPLFEFADVVER
                     GLGGLESHEVLRFVEPESGAVVSLRPDMTPQVARLLATRLASAPPPARLCYQGSVLRR
                     RQERARRHRQIPQAGIELIGREGPGGDLEVLSVAVAALRSTGLSDFTLDLGHARIAAA
                     LVEPVESNRAKEILDALEVKDETEVVRRAEQARLDSRALKALAELPSLHGGHEVWQKA
                     EQVLGLTAAEPAMRELKQLWEAASRAELAPQILVDLGETRDFAYYSGSLFQIHAEGPG
                     RPLGAGGRYDGLLDRFGAARPAVGFAFGLDDVAWALERASALSSPPPRILLASGATEL
                     APALRAAGIAAAAAPDGDEVLAYARSWRYSHVLEALGGGFSLLDSATQRRESLPKEPA
                     ALVSALAARLQVGAGS"
     gene            3722..4468
                     /locus_tag="VFQ35_24155"
     CDS             3722..4468
                     /locus_tag="VFQ35_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET9933828.1"
                     /translation="MTTGHETRVVDVRERGNDKNGQPITLDRRLFMQLLGFECDAAQP
                     LDAAAQALRDALAESKLPGVLYEDVNHPRGFAVLTLSEDPADFITKLRPHLAESTCRG
                     LVPRPELSMLGRTYSSGFEQDLEFWLLRRPRETVLNTAWQWAVWYPLRRKGPFERLDP
                     KEKGSIMREHGGIGRAYGETNLAHDVRLACHGLDQSDNDFVIGLIGPELYPLSHVVQS
                     MRQTRQTAEFMEKMGPFFVGRAVYRNPGTP"
     gene            4465..5145
                     /locus_tag="VFQ35_24160"
     CDS             4465..5145
                     /locus_tag="VFQ35_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9933829.1"
                     /translation="MSGGAAVEAVAFDLDGTLIDSRGDIAAATLHALTSHGFAPLPVE
                     TLLTYVGDGARPLLARSAGIAADDARLDALYASFLEFYTANPLVHTRFCRGAERALSD
                     LSSLKLALCTNKPRVTTLAVLEGLGILDLFAVIVAGNDLPRSKPDPLPIQHIAGALSV
                     APRRLVMVGDGAQDILAARGAGARAVGLRDGIQPIERLLAAKPEVLLDSLEELPSLIA
                     SWNAEPSS"
     gene            complement(5156..5332)
                     /locus_tag="VFQ35_24165"
     CDS             complement(5156..5332)
                     /locus_tag="VFQ35_24165"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HET9933830.1"
                     /translation="MSLLCPFCGEPDEIYVDLGGGTRQEYVEDCAVCCRPRVVHVEPG
                     GESGEVDVWLERGD"
     gene            complement(5362..>6693)
                     /locus_tag="VFQ35_24170"
     CDS             complement(5362..>6693)
                     /locus_tag="VFQ35_24170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933831.1"
                     /translation="VLVALGWLDNLYLVDIGFVSAVISVSADFTYRVTTDARRLQALS
                     TELTRQVEDRTRELVETRDNLVRAERLAALGRLSASVGHEINNPLSYVIGNLEYVSKE
                     LSQGRASQGVQEALRDAASGADRIRRIVHELRAFVRGTDRDRRELVDAREVLEAAVKL
                     VWSELRHRARLERDLKEVPKLYADPIRLTQVFVNVLMNAVQAIPEERAGRPESVITLR
                     TRELPSGLVAIEIEDTGIGISEADRPRLFEPFFSTKPQDKGTGLGLFVSLGIVSALGG
                     RIDVESHEGRGTLVRVVLPIPEGVVPVMERVSSLPPPSVKNRRLLVVDDDVLVARTLV
                     RLLSDHRVEVVTSGREAMAKLASEGNEFDLVLCDLMMPDVTGMDLYEEVEKKHPGLAG
                     RFVFISGGGVTERSRRFLEVHADRVLPKPIDSAQLIRLLAKRTARVDSPAA"
BASE COUNT         1137 a         2332 c         2201 g         1023 t
ORIGIN      
        1 ccgcccaaag acaagtaggc ctgcaggtgc gcgcgagcga gccgcgcgtc gcgaccgagc
       61 gccgcgtagc cgatggcggc gtggagctcg ccttcggcta aaaagggcgc ccgctcgctg
      121 aactcgaatt gcgagcgggc cgcgctcacc gctccaccga gagcggcctg cgcgacgcgt
      181 tcccgatcgc gtggcgacgc gaagcgctcg agcgagaagc cgccctccag cgcgcgagcg
      241 agcacttgag cttgttcgcc gttgcctgca cgatcgagac agctcgcgag cagcgccgtc
      301 gacaggtcgt cgagcccagg cgccgaggtg agccgccgcg cctcggacag atacagcacc
      361 gcatcgctga gcccctcggg cccggaccga gcgagcacgg acgctccctc caaggtcacg
      421 aggcgcacga gcaccggatc gttggtgaag gcgacgcgcg gaatcagcgc gcgatacgag
      481 gctgcggctt cgttcaagtg cccgctcacc tgggccacga taccgaactc acggagcgcg
      541 gcgacatcgc cgagcggccg ccgcggatcc gtcacgctgc ccgagagcgc cgcgtatgcc
      601 cccgcgaagt cgccgagccg cagtgcggca cgaccagcga ggactcgcgc ctccggctcg
      661 ttcggattca gcttctcggc ctcccgagcg agctcgagcg cccgctcggg agcgtcgcgc
      721 agccgcgcgt agccgaaggc gagcagcccg cagtagcgct ccgtcacgcc gccgcgggtc
      781 cgctcccaaa ggccattctc gtcgagcaac ggcgccggcc ggcacggggc tggccgcggg
      841 cttgccgccg caaacgcgcg tagcgctgcc acttccggcg ggcgcgccgg agcgccgagg
      901 agcgtgagct cgagcagcag cgccggcagc atgcgagcta cggcgccgca gcgcgcccga
      961 cgaagggatt gtgcagcacg atggtttcgt tgcgccgcgg accgacactg acgaggtaga
     1021 gcggcacttg gctctgctct tcgatgaagc ggacgtaggc gcgcgcggcg cgagggaggt
     1081 cgtcgagcac gcgagcattc tcgatgcgct cgctccagcc ctcgagctct tcgtagaccg
     1141 gcttgaccgt gttgccgagc tcgtgaagct catcgatcgg cagatcgacg acgcgaccgg
     1201 agggcgtatc gtaggcgacg cacaccttga tgcgagacat gcccgtgagc acgtcgagct
     1261 tggtgagcgc gagcgcgtcg aggccattca cgcgcgcggc gtaacgaagg ccgggcaggt
     1321 cgagccaacc ggtgcggcgg gggcggcccg taacggagcc gaactccacg ccgacctcgc
     1381 gcaagtggcg accgtcggcg tcgtcgagtt cggtggggaa cggccccgca ccgacgcgcg
     1441 tcgtgtacgc cttggtgatg cccagcacgg tattgatgcg gttcgggccg atgccggtcc
     1501 cgattgcggc gccgccggcg acggccgagc tcgacgtgac gtacgggtac gtgccgtgat
     1561 cgaggtcgag cagcgtgcct tgggcgccct cgagcatcac gcgctcgcgg cgacggatcg
     1621 cgctgtccac gagcagcgac gtgttgccga gcaagggcac taggcgcttc gccgcctttt
     1681 cgagcgagcc catgatttcg tcgacgttcg gcagatcgcc gcccagcgcg cgaatcgtcg
     1741 gtgcccaggc gtcgacggcg gcttcgatgc gggttcgcac gcggccaagg tcgcgcaaat
     1801 caccgagacg cacgccggtt cggcgcgcct tgtcttcgta cgcgggaccg atacccttct
     1861 tggtcgtgcc gatcgcgcgg tcgtccgcgg aagcctcgcg gagcccgtcg accaggatat
     1921 ggtacggcag aatcaagtga gcgcggtcac tcaagacgag gcgcttctcg acgtcagcgt
     1981 gaccgcgtcg aatgagcccg tcgatctcgt cgagcaagac gcccgggtcc accaccatcc
     2041 cctgccccag cacgcaacgc gtggagggtc gcaggatgcc gctcggtagc aagcggacga
     2101 cgatcttgtc gtctccgacg acgagcgtgt gccccgcgtt cggtccgccc gcgtagcgca
     2161 ccacgacgtc ggcgaactcc gtgtagatgt cgacgatttt tcctttgcct tcgtcgcccc
     2221 actgggcacc gacgatcact acggccgaca ttcgtttctt gtcctttcaa gaacccgcgc
     2281 cgacttggag tcgggcggcg agagcagaga ccagcgctgc cggttccttg ggcaacgact
     2341 cacggcgctg ggtggcggag tcgagcagag aaaagcctcc gccgagcgct tcgagcacgt
     2401 gcgaatagcg ccacgaacgc gcgtaggcca gaacttcgtc gccgtccggc gcggcggccg
     2461 cagcaattcc tgcggctcgt aaggccggtg cgagctcggt ggcgccggaa gccagcaaaa
     2521 tgcgaggcgg gggcgagctg agcgcggaag cccgctcgag cgcccaagcg acgtcgtcca
     2581 gcccgaacgc aaagccaacg gcgggacgcg cggcgccaaa ccgatccagc aagccgtcgt
     2641 aacgcccacc ggcgccgagc ggccgtcctg gaccttcggc gtgaatctga aagagcgagc
     2701 cgctgtagta agcgaagtct ctggtttcgc cgaggtccac caagatttgc ggcgcgagct
     2761 cggctcggga cgccgcctcc cagagctgct tcagctcgcg catcgccggt tcggccgcgg
     2821 tgagcccgag cacctgctcg gctttttgcc aaacctcatg gccgccgtgc aaagaaggaa
     2881 gctcggcgag cgccttcagg gctcgcgagt cgaggcgcgc ttgttctgcc cgacgaacga
     2941 cctcggtctc gtccttgacc tcgagcgcgt cgaggatctc tttggcgcgg ttcgactcca
     3001 ccggttcgac cagcgccgcc gcgatgcggg cgtgcccgag atcgagcgtg aagtcactga
     3061 ggccggtgct gcgcagagcc gctacggcca cgctcagcac ctcgagatcg cccccgggcc
     3121 cttcgcgccc gatgagctcg atgcccgcct gaggaatctg ccggtgacgt cgagcgcgct
     3181 cttggcggcg acgcaacacc gagccctggt aacaaagcct ggccgggggc ggcgccgacg
     3241 cgagccgcgt cgcgagcaac cgcgccacct gcggtgtcat atccggacgc agcgaaacca
     3301 cggctccgct ctccggttcg acgaaccgga gcacctcgtg cgactcgagc cccccgagcc
     3361 cacgctccac gacgtcggcg aactcgaaca gcggtacgac cacgagctcg tacccggcga
     3421 gctcgaaggc gcgcaagatg cgccgcgcca aatctgcgtg tcgccaagcc tcgggcggaa
     3481 gaaggtcccg catccccgcg ggaaccgggt gcgagagcgc gtagaccccg gcggacgttg
     3541 ctggatcagc gggggggaaa taggagcttt ccggcacagc cggccgagtc tatcaaagaa
     3601 atcagagaag caaggcgcga ggcgtcgctt tctgatcatt caccattcac cgagtcgtcc
     3661 ctttccgaga acgaggcgac ggacaggcgg cccggcgtgt gttagttctc tcggcaaccg
     3721 catgaccacg ggccacgaaa cgagagtcgt cgacgtgcgc gagcgcggca acgacaaaaa
     3781 cgggcagccg atcacgctgg atcggcgcct cttcatgcag cttttgggct tcgagtgcga
     3841 cgcggcgcag ccgctcgacg cggcggccca agccctgcgc gacgcactcg ccgaaagcaa
     3901 gcttccagga gtcttgtacg aggacgtcaa tcatccgcgc gggttcgcgg tgctcacgct
     3961 gagcgaggac ccagccgact tcatcacgaa actgcggccg cacctcgccg agtcgacttg
     4021 tcgaggcctc gtccctcgac ccgagctcag catgctgggg cggacgtact cgagcggctt
     4081 cgagcaagac ctcgagtttt ggcttttgcg gcggccgcgc gagaccgtgc tgaacaccgc
     4141 gtggcagtgg gccgtttggt acccgttgcg ccgcaagggt cccttcgagc gactcgaccc
     4201 caaagagaag ggcagcatca tgcgcgagca cggcggcatc gggcgcgctt atggcgaaac
     4261 gaacctcgcg cacgacgttc ggctggcgtg ccacgggctc gatcagagcg acaacgactt
     4321 cgtcatcggg ctcatcggac cggagctcta ccccctttcg cacgtcgtgc agtcgatgcg
     4381 ccagacgcgc cagaccgcgg agttcatgga gaagatgggg ccgttcttcg tcggccgcgc
     4441 cgtgtatcga aacccgggca ctccttgagc ggaggggcag cggtcgaggc cgtcgccttc
     4501 gacctagacg gcactctcat cgattcgcgt ggcgacatcg ccgcggccac gcttcacgca
     4561 ctcacgagcc acggctttgc gcccctgccc gtcgagaccc tgctgactta cgtcggggac
     4621 ggagcgcggc cgttgctcgc ccgctccgca gggatcgccg cggacgacgc gcgactcgac
     4681 gcgctctacg cgagcttcct cgagttttac acggcaaacc ccctcgtcca cacgcggttt
     4741 tgccgtggcg ccgagcgagc cctgtcggac ctgtcgtcgt tgaagctggc tctttgcacc
     4801 aacaagcccc gcgtcacgac cctcgccgtg ctcgaagggc tcggtattct cgacctcttc
     4861 gccgtgatcg tagccggcaa tgacctgccc cgctcgaagc ccgacccgct tccgatccag
     4921 cacatcgccg gagcgctgag cgtcgctcca cggcgcctcg tcatggtcgg cgacggcgcg
     4981 caagacatcc tcgctgcgcg cggcgctggc gctcgcgcgg tcgggttacg cgacggcatc
     5041 cagcccatcg agcgcttgct cgcagccaag ccggaagtct tgctcgacag cctcgaagag
     5101 ctgccttcac tcatcgcgag ctggaacgcc gagccctctt cctaatcggg ccgcgtcagt
     5161 caccgcgctc gagccacacg tcgacttcac ccgactcgcc cccgggctcg acgtgaacca
     5221 cgcgcggacg gcagcagacg gcgcagtctt cgacgtattc ttgccgcgtg ccgccaccga
     5281 ggtcgacgta gatctcgtcc ggctccccgc aaaacgggca gagaaggctc attcggcgag
     5341 cgcgtcttcg tttcttgtcg ttcatgcggc gggtgaatcg acgcgagcgg tccgcttcgc
     5401 gaggaggcga atcagctgcg cgctgtcgat cggtttcggc aaaacgcgat cggcgtgcac
     5461 ctcgaggaag cgacgcgagc gctcggtgac cccgccgccg ctgatgaaca cgaagcgccc
     5521 cgcgaggcca ggatgcttct tttcgacctc ctcgtagaga tccatcccgg tcacgtccgg
     5581 catcatcaaa tcgcagagca ccaagtcgaa ttcgttgcct tcgctcgcga gcttcgccat
     5641 cgcttcgcga ccgctcgtca ccacctcgac acgatggtca ctcaacaggc gcacgagggt
     5701 ccgtgcaacc aagacgtcgt cgtcgaccac gagcaagcgc cgattcttca ccgacggtgg
     5761 cggcagagac gacacgcgct ccatgacggg gaccacgccc tctgggattg gcagcacgac
     5821 gcgcacgagg gtcccccggc cctcgtggct ctcgacgtcg atgcgcccac cgagcgcgct
     5881 cacgatcccg agcgagacga acaagccgag gccggttccc ttgtcctggg gcttcgtcga
     5941 gaagaacggc tcgaacaagc gcgggcgatc cgcctccgag atcccgatgc cggtgtcttc
     6001 gatttcgatc gcgacgagcc cgctcgggag ctctcgcgtg cgcagcgtga tcaccgactc
     6061 cggacgaccc gcgcgctcct cggggatcgc ttgaacggcg ttcatcagaa cgttcacgaa
     6121 aacctgcgtc aaccggatcg gatccgcgta gagcttcggc acctctttca aatcgcgctc
     6181 gagcctcgcg cggtgccgca gctcgctcca cacgagcttg accgctgcct cgagcacttc
     6241 gcgcgcgtcg acgagctcgc gccgatcgcg atccgtcccg cgcacgaagg cgcgaagctc
     6301 gtgcacgatg cgccgaatgc gatcggcgcc gctcgcggcg tcgcgcagcg cctcttgcac
     6361 tccctgcgac gcgcgaccct ggctcagctc tttggagacg tattcgaggt tgccaatcac
     6421 atacgagagt ggattgttga tctcgtgtcc gacgctcgcg ctcagccgac cgagcgcggc
     6481 gagtcgctct gcccgcacga ggttgtcgcg cgtctccacg agctcgcgag tgcgatcctc
     6541 cacctggcgc gtgagctcgg tcgaaagggc ctgcagccgt cgcgcgtcgg tggtcacgcg
     6601 atacgtgaag tcggccgaca ccgaaatcac ggccgacacg aagccgatat cgacgaggta
     6661 gagattatcc agccagccga gcgcgacgag gac
//
LOCUS       DASTKS010001139         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1073897, whole genome shotgun sequence.
ACCESSION   DASTKS010001139 DASTKS010000000
VERSION     DASTKS010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073897"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFQ35_24175"
     CDS             complement(<1..494)
                     /locus_tag="VFQ35_24175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9933832.1"
                     /translation="MKLLYLMTEPFGIGGVQSDILTLSEDLTKKGHEVFVATTAGELL
                     EELKSKGARHLDIDFNFSGVRGVERAGRALRDAIREHGIELVAPQSVRSIIATYAALR
                     LVPFKYRTASGRRPPIVATIHNIHNPMHFRYAGHILQRCADYVIFESHYERDRCMASG
                     LSAD"
     gene            complement(508..1365)
                     /locus_tag="VFQ35_24180"
     CDS             complement(508..1365)
                     /locus_tag="VFQ35_24180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9933833.1"
                     /translation="MSTSLRGRARQLLPYLFLPATYSRQLIQPAVRILMYHRVARLPA
                     YDQLTVTPERFDEQLEELATNFRVVSLADAFAELERGKPRETLVVVTFDDGYLDNLTN
                     ALPILRRHAIPATIFVTARFADGNTRHPRYVNESGRLHLDWSEIGELAKEPGITIGSH
                     TLTHPFLSRLDASAARDEIAGSREEIGRHSGKDVEFFCYPSGDFTAREEQLVANAGYR
                     AAVSVHPGANRNTNHRFALRRTEVTDRDSARELRLKLQGAYDPLHLLLHWRRERGFER
                     ARGDQSTYP"
     gene            complement(1362..2504)
                     /locus_tag="VFQ35_24185"
     CDS             complement(1362..2504)
                     /locus_tag="VFQ35_24185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9933834.1"
                     /translation="MNDHAPHVVHVIDELPPDGAERLLADMLRHRSDRFRFSVLCLIR
                     AGGLADEIRAMGVPVELIERKGKFDPTLIWRLATWFRRNKVDVVHTHLFTADSYGRIA
                     ARLAGVRAVFSTAHNTVVCEGKPQKAVHWALSWISNSVVACSEEVGRVMRSQDHLPAS
                     RLKVIANGINIDRFANANGEGVREEFGVPDGVPLIGVVGRLHPAKGHVDLIAAIAELR
                     DAGVGLKCLIIGSGEIRGELEQEVRRLNLEDRIVFTGQRKDVPRLLAGLDIIAMPSRW
                     EGLPLALLEAMAMARPIVATRVSGIPDVIRDGENGLLVPAAQPKEFAAALRRLFESPE
                     LRQRLGTNALATLRRDYDVKRTGRAYEALYLEALGLPQDQAAVGTA"
BASE COUNT          415 a          813 c          886 g          456 t
ORIGIN      
        1 ttgtcggcgc tgagaccgct cgccatgcag cgatcgcgct cgtagtgcga ctcgaagatc
       61 acgtagtcgg cgcagcgctg caggatgtgc cccgcgtagc ggaagtgcat cgggttgtgg
      121 atgttgtgga tcgtggccac gatcggcggc cggcgcccgc tcgcagtgcg gtacttgaac
      181 ggcacgaggc gcagcgcggc gtaggtcgcg atgatggagc ggaccgactg cggcgcgacg
      241 agctcgatgc cgtgctcgcg aatcgcatcg cgcaacgcgc ggccggcacg ctccacgccg
      301 cgtacgccag agaagttgaa gtcgatgtcg aggtggcgag cgcccttcga cttgagctct
      361 tccaagagct cacccgccgt cgtcgccacg aagacctcgt gccccttttt ggtgagatct
      421 tcggacaagg tgagaatgtc ggactggacc ccgccgattc cgaagggctc cgtcatcaag
      481 tacagaagtt tcatggcaat ccgctcgtca gggataggtg gattggtcgc cgcgcgcgcg
      541 ttcgaagccc ctctcgcgcc gccagtggag caggagatgg agcgggtcgt aagcgccctg
      601 cagcttcaga cgtagctcgc gcgcgctgtc gcgatcggtg acttcggtcc ggcgcagcgc
      661 gaagcgatgg ttggtatttc gattggcacc cgggtggacg ctgaccgcgg cgcgataacc
      721 cgcgtttgcg accagctgct cttctcgcgc cgtgaagtcc cccgatggat agcagaagaa
      781 ctcgacgtcc ttgccgctgt gccgcccgat ttcttcccgg ctgcccgcga tttcgtcgcg
      841 cgccgcgctc gcgtcgaggc gcgagaggaa ggggtgcgtc agcgtgtgcg agccgatcgt
      901 gatcccgggc tccttcgcga gctctccgat ttcgctccag tcgaggtgaa ggcgccctga
      961 ttcgttcacg tagcgcgggt gacgcgtgtt tccgtcggcg aagcgagcgg ttacgaagat
     1021 cgtggcgggg atcgcgtggc gacgcaggat gggcagcgcg ttggtgaggt tgtcgaggta
     1081 gccgtcgtcg aaggtgacga ccacgagggt ctcgcgtggc ttgcctcgct cgagctcggc
     1141 gaaggcgtcg gcgaggctca cgactcgaaa gttggtcgcc aattcctcga gctgctcgtc
     1201 gaagcgctcg ggcgtcaccg tgagctgatc gtacgcgggc aggcgcgcca cgcgatgata
     1261 catgaggatg cgcacggccg gctgaatcag ctgccgcgag taggtggccg gcaggaacag
     1321 gtacggtaag agctgccgcg cgcggccccg aagcgacgta ctcatgcggt cccgaccgcg
     1381 gcttgatctt gtggcaaccc gagcgcttcg aggtaaaggg cctcgtaggc gcggccggtg
     1441 cgctttacgt cgtagtcacg tcgcaaagtc gcgagcgcgt tggtgccgag gcgttgccgc
     1501 agctccggcg actcgaacaa gcgccgcagc gcggccgcga actctttggg ctgcgcggcg
     1561 ggcacgagga gcccgttttc tccgtcgcgg atcacgtcgg ggatcccgct cacgcgagtc
     1621 gcgacgatgg gtcgtgccat cgccatcgcc tcgagcaacg cgagcggcaa gccctcccaa
     1681 cgcgacggca tggcgatgat gtcgagccct gccagcaagc gcggcacgtc tttacgttgg
     1741 cccgtgaaca cgatgcgatc ttcgaggttc agccgccgaa cctcttgctc gagctcgccg
     1801 cgaatctcgc ccgacccgat gatgaggcat ttgagcccca cccccgcgtc gcgcagctcc
     1861 gcgatcgcgg cgatcaagtc gacgtggccc ttggccgggt gcaggcgtcc cacgactccg
     1921 atcagcggga cgccgtcggg tacaccgaac tcttcgcgca ctccttcgcc gttcgcgttc
     1981 gcgaaccgat cgatgttgat gccgttcgcg atcaccttca ggcggctagc gggcaggtga
     2041 tcttggctgc gcatcacgcg cccgacctct tcggagcagg cgaccacgct gttcgaaatc
     2101 caggagagcg cccaatggac ggctttctgc ggcttgccct cgcagacgac ggtgttgtgc
     2161 gcggtcgaga acaccgcgcg cactcccgcg agacgcgcgg cgattcggcc gtagctgtcg
     2221 gcggtgaaga ggtgagtgtg caccacgtcg accttgttgc gccgaaacca agtggcgagg
     2281 cgccagatga gggtcgggtc gaacttgccc ttccgctcga tgagctcgac cggaactccc
     2341 atcgcccgga tctcgtcggc gagaccgcct gcgcggatca agcagagcac gctgaagcgg
     2401 aagcggtccg agcggtgccg cagcatgtca gccaggagcc gctcggcccc gtctggtggc
     2461 agctcgtcga tcacgtgaac tacgtgaggc gcgtggtcgt tcatttcatt ctccgcgtac
     2521 gagcggccga gtgtagtcgg aggctgccgg cgaagctata tgccgtgacc
//
LOCUS       DASTKS010001140         6069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1073974, whole genome shotgun sequence.
ACCESSION   DASTKS010001140 DASTKS010000000
VERSION     DASTKS010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073974"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFQ35_24190"
     CDS             <1..894
                     /locus_tag="VFQ35_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9933835.1"
                     /translation="ALGLNAAGGNIGVSTVQLLVPIVIGYTLLPGSSGALHLENAGLL
                     WLPLIVLSAIGAFFFMNNLVNARSNFKDQIVVAQQRHTWIMSFLYIGTFGSFVGYSAA
                     FPLLMKTQFPEVTTNLAFLGALVGSVARPLGGKLADRIGGARLTFWNFVAMTASSVGL
                     LWAVEQRNLPAFLLVFLTLFMTTGVGNGSTFRMIPVIFRTIHLKRVAGQGAEAEAKAL
                     VAARRETAAVIGLAAAIGALGGYFIPRAFGASIKATGGPALAVSYFVGFYALCVATTW
                     WCYLRSSFLVKRIPSLASAGA"
     gene            954..3536
                     /gene="nirB"
                     /locus_tag="VFQ35_24195"
     CDS             954..3536
                     /gene="nirB"
                     /locus_tag="VFQ35_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HET9933836.1"
                     /translation="MSTNGKSKPGLVVVGNGMVGHRFLQEAVRRGLNQSHHVIAFAEE
                     SRLAYDRVNLSKYFDGKTAEDLCLVLPREYEEGGVELVPGDAVVGIDRDARVVHTKSG
                     KSLVYETLVLATGSRPFVPEIPGRDAEGCFVYRTIDDLELIRDFAGRSRTGVVIGGGL
                     LGLEAAGALRALGLDTHVVEFAPRLMPLQVDEVGGVVLRQRIEELGVKVHVERSTSEI
                     MRDASGRVVGLRFADGAELLADMVVFSAGIRARDELARSFGLALGERGGIAIDAECRT
                     SDPHIFAVGECASFSGKTYGLVAPGYRMAEVAASVIAGQAARFDGFDMSTKLKLLGVD
                     VGSFGDAFARTPGARTISLFDSVAGVYKKIVLSADKKFILGGVLVGDASAYSELLSYA
                     QNAIAAPEHPEELLVPPREGGKKGLGVDSLPEAAMICSCNNVDKGTICKTIREQKLTT
                     LGSVKKCTKAGTSCGSCATLVDDLLKIELKRAGVAVVNHLCEHFKFSRQELFHLVQVS
                     QIKTFAELLHAHGSGEGCEICKPAVASILASVWNEYILKKEHAGLQDTNDRFLANIQR
                     DGTYSVVPRLAAGEVTPDQLIVLGRVAKKYGLYTKITGGQRIDLFGARLEQLPLIWGE
                     LVEAGFESGHAYGKALRTVKSCVGSTWCRYGVQDSVGMALRIENRYKGLRSPHKLKGA
                     VSGCARECAEAQGKDFGVIATEKGYNLYVCGNGGMKPQHAQLLAADLDGDTLVKYLDR
                     FLMFYVKTADRLQRTATWLNKLEGGLEYLKQVVVEDSLGIGAELEAQMERIVDTYQCE
                     WKTTVNDPEKLKLFRAFVNTDESDPSIVVVSERGQNRPAFWDEKLPLVEAGRLRLPVL
                     QEAM"
     gene            3533..3958
                     /gene="nirD"
                     /locus_tag="VFQ35_24200"
     CDS             3533..3958
                     /gene="nirD"
                     /locus_tag="VFQ35_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET9933837.1"
                     /translation="MKGSSMLAGNEATRSEGGIARFSVVAEEQIWVDVCALDDVIPGT
                     GVAALIAGEQVAVVRSPNGESVFAISNFDPFSKAFVLARGIVGDRAGTLKIASPIYKQ
                     NFDLKTGVCLDDPSVRLPVYGTKVEAGRIFVARRSGESK"
     gene            3955..5646
                     /locus_tag="VFQ35_24205"
     CDS             3955..5646
                     /locus_tag="VFQ35_24205"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9933838.1"
                     /translation="MTMAATQVLPRPSEDARDGKRRLPVLPVAALLDAQRTLTAVERF
                     SQQHDEAGLPAQAKYYRDLIPLERPKAGEQYAFEVDLDACTGCKACVAACHTMNGLDS
                     TEVWRTVGLLHGGTSAAPALQTVTTSCHHCVEPACLAGCPVQAYEKDPVTGIVKHLDD
                     QCFGCQYCTLMCPYDAPKYNKARGVVRKCDMCSDRLEHGEAPACVQACPNEAIAIRVV
                     ERATVVESSVAHAFLPGAPAPEHTLPTTVYKTEKRMPPNMLPADFYRTAPEHSHPPLV
                     VMLTLTQLAVGAFGLCYLVETVSGQSAGSPLAQTVFACTLAFVALGASVFHLGRPWLA
                     WRAFLGLRTSWLSREAVAFGIFAKLAVLYGVLAAAPLLPEFPGKSTALALSPVVQAAA
                     ALVGALGVFCSVMVYVATRRAQWAGPQTGIKFFGTALALGGAAVFAVYAFTGGTLDRV
                     GTALLSLVGGVMTVKLAFEASALFQVRDRRLSVMKRMALLMLGDLKAVTYLRFALGVA
                     GGLALPALMLRGSVARELLPSFSAVTLALLVAGELFERYLFFRAAPASRMPGGIR"
     gene            5643..>6069
                     /locus_tag="VFQ35_24210"
     CDS             5643..>6069
                     /locus_tag="VFQ35_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9933839.1"
                     /translation="MTILGDLGKKGLDLLIRKDGPLTRELLLKLAKFGLGRLPDRLAA
                     EQTTTMVCGFCSTGCGLNLLLKKGEAVGLMPATEYPVNLGMACPKGWEALTPLRGPGR
                     ATRPLVKRGGRLVEVSWEEAVFEMVDRFKAIQEKHGPAAV"
BASE COUNT         1023 a         1906 c         2044 g         1096 t
ORIGIN      
        1 gcgctcggct tgaatgcagc gggtggaaac atcggtgtga gcacggttca gctgctggtg
       61 cccatcgtga tcggctacac gctgttgccg ggctcatcgg gcgcactgca cctcgagaac
      121 gccggcctgc tgtggcttcc gctcatcgtg ctctcggcca tcggcgcgtt ctttttcatg
      181 aacaacctcg tgaacgcgcg ctcgaatttc aaagaccaga tcgtggtcgc gcagcaacgc
      241 cacacctgga tcatgtcgtt cctgtacatc ggcacgttcg gctcgttcgt gggctactcg
      301 gcggcgttcc cgttgctcat gaagacgcag ttccccgagg tgacgacgaa cctcgcgttc
      361 cttggtgcgc tcgtgggttc agtcgctcgt cctctcggtg gcaagctcgc cgatcgcatc
      421 ggcggcgccc gactcacttt ctggaacttc gtggcgatga cagcttcgag cgttggcctc
      481 ctgtgggcgg tggagcaacg gaacctgccg gcgttccttt tggtcttctt gaccctgttc
      541 atgaccacgg gcgtgggcaa cggctcgacg ttccgcatga ttccggtgat cttccggacg
      601 attcacctga agcgcgtggc ggggcagggc gcggaggcgg aagcgaaggc gctcgttgcg
      661 gcacggcgtg agacggccgc cgtcatcggg ctcgcggcag cgatcggcgc gctcgggggg
      721 tacttcattc cgcgcgcgtt cggggcgtcg atcaaggcga ctgggggacc tgcgctcgcg
      781 gtgagctact tcgtcggctt ctacgcgctg tgcgtagcca cgacatggtg gtgttaccta
      841 cgcagcagct tcctcgtaaa gcgcatcccg agcctggcga gcgctggcgc gtgagagctg
      901 aaggacgcgc cttcgacacc aattcgaaac gaaaaacagt cgaggatcga ttcatgtcaa
      961 cgaacggaaa gagcaagccg gggctcgtcg tcgtcggaaa cggaatggtg ggccaccgct
     1021 tcctacaaga agccgtgcgc cgaggcctca accagagcca ccacgtgatc gcgttcgccg
     1081 aggagtcgag gcttgcttac gatcgcgtga acctctccaa gtacttcgac ggcaagaccg
     1141 cggaggactt gtgtctggtt ctcccgcgcg agtacgaaga aggcggcgta gagctcgtgc
     1201 cgggcgatgc agtggtcggc atcgatcgcg atgcgcgcgt ggtgcacacc aagtctggca
     1261 agtcgctcgt atacgagacg ctcgtcctcg caaccgggtc acgcccgttc gtacctgaga
     1321 ttccggggcg cgacgcggag ggctgcttcg tgtaccgcac catcgacgac ctcgagctga
     1381 tccgcgactt tgcggggcgc tctcggaccg gcgtcgtgat cggcggcggt ttgctcggcc
     1441 tcgaagcggc gggagcgcta cgggcgctcg ggctcgatac ccacgtcgtc gagttcgcgc
     1501 cgcggctgat gccgttacag gtagacgagg tcggtggtgt cgtgttgcgc cagcgcatcg
     1561 aagagctcgg cgtgaaggtg cacgtcgagc gctcgacctc cgaaatcatg cgcgacgcca
     1621 gcggccgcgt cgtcggcctc cgcttcgcgg acggcgcaga gctgctcgcc gacatggtcg
     1681 tcttctccgc aggtatccgc gcgcgcgacg agctcgcgcg ttctttcggg ctcgcgctcg
     1741 gcgagcgtgg cggcatcgcg atcgacgcgg agtgccgcac cagcgacccg cacattttcg
     1801 cggtgggcga gtgcgcttcg ttcagcggca agacttatgg cttggtcgcg ccggggtatc
     1861 gcatggccga ggtcgcggcg agcgtcatcg ccggccaagc ggcgcgcttc gatggcttcg
     1921 acatgagcac gaagctgaag ctcctcggcg tggacgtcgg gagcttcggc gacgccttcg
     1981 cgcggacgcc gggcgcgcgc accatcagcc tcttcgacag cgtcgcgggc gtctacaaga
     2041 agatcgtgct cagcgccgac aagaagttca tcctcggcgg cgtgctggtc ggcgacgcat
     2101 ccgcgtactc cgagctcttg agctacgcgc agaacgcgat cgccgcgccc gagcaccccg
     2161 aagagctgtt ggtgccgccg cgcgaaggcg gaaagaaggg cctcggcgtc gactcactgc
     2221 ccgaggcggc gatgatttgc tcctgcaaca acgtcgacaa aggcaccatc tgcaagacca
     2281 tccgcgagca gaagctcacc acgctcggca gcgtgaagaa gtgcacgaag gccggcacga
     2341 gctgcggctc ttgcgcgacc ctcgtcgacg acctactcaa gatcgagctc aaacgcgccg
     2401 gtgtcgccgt cgtcaatcac ctgtgcgagc acttcaagtt cagccgacaa gagctctttc
     2461 acctcgttca ggtgagccaa atcaagacct tcgcagagct cttgcacgcc cacgggagcg
     2521 gggaaggctg cgaaatctgc aagcccgcgg tcgcctcgat tctggcttcg gtgtggaacg
     2581 agtacatcct gaagaaggag cacgcggggc tccaagacac gaacgaccgc ttcctcgcga
     2641 acatccagcg cgacggcacc tattcggtcg taccgaggct cgcggcgggc gaagtaactc
     2701 ctgaccagct catcgtgctc ggtagggtcg cgaagaagta cggcctctac actaagatca
     2761 ccggcggtca gcgcatcgat ctcttcggcg cgcggctcga gcaactgccg ctcatctggg
     2821 gcgagctcgt cgaggccggc ttcgagtctg gtcacgccta cggcaaggcg ctgcgcaccg
     2881 tgaaatcgtg cgtggggagc acgtggtgcc gctatggcgt ccaggactcc gtcggcatgg
     2941 cgctccgcat cgagaatcgt tacaagggac tgcgcagccc gcacaagctc aaaggcgcgg
     3001 tgtccggctg cgcgcgcgag tgcgccgagg cgcaaggcaa agacttcggg gtcatcgcca
     3061 ccgagaaggg ctacaacctc tacgtctgcg gcaacggcgg catgaagccg cagcacgcgc
     3121 agttgctcgc cgcggatctg gacggcgaca cgctcgtcaa atacctcgat cggttcttga
     3181 tgttctacgt caagacggcg gatcgcttgc agcgcacggc cacgtggctc aacaagctcg
     3241 aaggcgggct cgagtacctg aagcaagtcg tggtggagga ctcactcggc atcggcgcgg
     3301 agctcgaggc gcagatggag cgcatcgtcg acacttatca gtgcgaatgg aagacgaccg
     3361 tcaacgatcc ggaaaagctc aagctgttcc gcgccttcgt gaacaccgac gagagcgatc
     3421 cgagcatcgt ggtcgtgtcc gagcgcggac aaaaccgccc cgcgttctgg gacgaaaagc
     3481 tcccgctagt cgaggcgggc cggctccgct tgcccgtgct gcaggaggcg atgtgaaagg
     3541 ttcatccatg ttggcaggca acgaagcaac tcgaagcgaa ggcggtatcg cgaggttctc
     3601 ggtcgtggca gaggagcaga tttgggtaga cgtttgtgct ctggacgacg tcatccccgg
     3661 cacgggcgtc gcggcgctca tcgccggtga gcaggtcgcg gtcgtgcgtt cgccgaacgg
     3721 tgagagcgtg ttcgccatct cgaacttcga tcctttcagc aaggccttcg tgctggcacg
     3781 aggcatcgtc ggcgatcgcg cgggcacgct caaaatcgct tctccgatct acaagcagaa
     3841 cttcgatttg aagaccggcg tctgcctcga cgatccgagc gtgcggcttc ccgtatacgg
     3901 aacgaaggtc gaagcggggc gtattttcgt cgcgagacgc tcgggcgaat ccaaatgacg
     3961 atggccgcaa ctcaggtgct cccgcgcccg agcgaagacg ctcgcgacgg aaagcgccgg
     4021 ctccccgtgc tgcccgtcgc cgccctgctc gacgcgcaga ggacgctcac tgcggtcgag
     4081 cgcttctcgc agcaacacga cgaagccggg ctgcccgcgc aggcgaagta ctatcgcgac
     4141 ctcatcccgc tcgagcgacc gaaagccggt gaacagtacg ccttcgaggt cgacctcgac
     4201 gcatgcacgg gctgcaaggc ctgcgtcgct gcctgtcaca ccatgaacgg gctcgactcg
     4261 acggaggttt ggcgaacggt gggcttgttg cacggcggaa cgagcgccgc ccccgcgctc
     4321 caaaccgtga ccacgtcttg tcatcactgc gtggagccag cgtgcctcgc gggttgcccc
     4381 gtccaagcct acgaaaagga tcccgtcacc ggaatcgtga agcacttgga tgatcagtgc
     4441 ttcggttgcc agtactgcac gctcatgtgc ccctacgacg cgcccaagta caacaaggcg
     4501 cgcggcgtgg tgcggaaatg cgatatgtgc agcgaccgcc tagagcacgg tgaagcgccc
     4561 gcgtgcgttc aggcctgccc caacgaagcg atcgcgatcc gcgtcgtcga gcgcgcgacc
     4621 gtcgtcgagt cgagcgtggc gcacgccttc ttacccgggg ctccggcgcc agaacatacg
     4681 cttccgacca ccgtctacaa gacggagaag cgcatgccgc cgaacatgct gccggctgat
     4741 ttctaccgca ccgctcccga gcactcgcac cctccgctcg tggtcatgct gacactcacg
     4801 cagctcgcgg tcggcgcgtt cgggctttgt tatttggtcg aaacggtctc ggggcaaagc
     4861 gcggggagcc cgctggctca gacggtcttc gcgtgtacgc tcgctttcgt cgcgctcggc
     4921 gcgagcgtct ttcatttggg gcggccgtgg ctcgcgtggc gcgccttctt ggggctgcgg
     4981 acgtcttggt tgagtcgcga ggcggtcgcg ttcggaattt ttgcaaagct cgctgtgctc
     5041 tacggggtgc tcgcggcggc cccgctcttg ccggagtttc caggtaagag cacggcgctc
     5101 gcgttgagcc cggtagttca ggcagcggcg gcgctggtgg gtgcgctcgg cgtgttctgc
     5161 tccgtgatgg tttacgtagc gacgcgccga gcgcagtggg cgggccctca aacgggcatc
     5221 aagttctttg gaactgcgct cgcgctcgga ggagcagccg tcttcgcggt gtacgccttc
     5281 acgggtggga ccctcgatcg ggttgggacg gcgctcctct cgctggtcgg cggcgtgatg
     5341 acggtgaagc tcgcgttcga ggcgagcgcg ctctttcagg tgcgagaccg gcgcctgagc
     5401 gtgatgaaac ggatggccct attgatgctc ggcgacctga aggcggtgac ttacctgcgg
     5461 tttgcgctcg gggtcgctgg cgggctcgcg ctgccagcgt tgatgctgcg cggaagcgtc
     5521 gctcgcgagc tccttccgag cttctcggcg gttacgctcg cgctcttggt cgcgggtgag
     5581 cttttcgagc ggtacctgtt cttccgcgcg gctccggcgt cgcgcatgcc gggaggcata
     5641 cgatgacgat tctgggtgac ctcggcaaaa agggcctcga cctgctgatc cgcaaagacg
     5701 ggccgctcac gcgcgagctc ctgctcaagc tcgccaagtt cgggctggga cgcttgccgg
     5761 atcgtctcgc cgccgagcag acgaccacga tggtgtgcgg cttctgctcg accggctgcg
     5821 ggctgaatct gctgctcaag aagggagagg cggtcgggtt gatgccggcc accgaatacc
     5881 ccgtgaacct tggcatggcg tgccccaagg ggtgggaggc gctgacgccg ctgcgggggc
     5941 ccgggcgcgc gacgcggccg ctcgtcaagc gtggcggtcg cctagtcgaa gtgagctggg
     6001 aagaggccgt cttcgagatg gtggaccgct tcaaggcgat tcaggagaag cacggtccgg
     6061 cggcggtcg
//
LOCUS       DASTKS010001141         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1074130, whole genome shotgun sequence.
ACCESSION   DASTKS010001141 DASTKS010000000
VERSION     DASTKS010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074130"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFQ35_24215"
     CDS             <1..517
                     /locus_tag="VFQ35_24215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9933840.1"
                     /translation="HPGNIERREIPEVRVIDVAKSDSLDDANATTPRRTFAVAGGTAC
                     FGDSGGPALSMTSGALVGVYSRITGDCFAVESRNTFMQAASFSDLFTRAFELVGEQPA
                     LEPGPEAPPSSGAAGAPATAPAPQDGRHEAFRCAVAVPGDQSGSSFGATSVFALVLFW
                     LTRRRREKSPF"
     gene            complement(483..1964)
                     /locus_tag="VFQ35_24220"
     CDS             complement(483..1964)
                     /locus_tag="VFQ35_24220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9933841.1"
                     /translation="MIRPTDDRSPGGTTEAPPAPESGPYVLTIHAPGRPPRSMTLEPG
                     KEYSVGRRKSASIVLDHAWVSERHAVITAADPPYIVDVGSRNATFVDGHRLVVDAPKP
                     LPSGSVVSIGGFSMLITRAEHPETSAVMRCAQPVQLPSEPPPTTQPPDLGESAPVVRD
                     QRMRELYTFAQQIARSSVSVLIMGETGSGKELMAQAIHRQSPRRRRALVSLNCATIPE
                     TLVESELFGYERGAFSGAVAAKRGLFEEANGTTFFLDEIGELPASVQAKLLRVLETGE
                     VLRLGAVRPVHVDVRIVAATNRDLATQVKKGSFRADLFYRLNGLTISIPPLRERPDDV
                     EDLARHFAQKAGEGHVEFSTAALDALRDYRWPGNVRELKSVIERALLISEGGVIEVGA
                     LNLQRSTTPPEAPPPESLAKPEATEIEQTERFSADELPTSVFRAELDRREQRRIREAL
                     ERTAGNQTEAARLLNISRRTLMKRMDRFGMSRPRKGSSRGGDE"
     gene            complement(2210..2518)
                     /locus_tag="VFQ35_24225"
     CDS             complement(2210..2518)
                     /locus_tag="VFQ35_24225"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HET9933842.1"
                     /translation="MPDLDQSADGATITLSVGESRSLVLGERPTTGFRWHVAVSNREL
                     LEVADTFRPRSLKPGGGGERVFTISALKRGASTVTWSHQRGTSEATADRSITVTFQIT
                     "
     gene            complement(2532..>2969)
                     /locus_tag="VFQ35_24230"
     CDS             complement(2532..>2969)
                     /locus_tag="VFQ35_24230"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HET9933843.1"
                     /translation="ANPDTDLFPSDAKAVQKPSNDAYGEAEQYRVLAYSRVPQSLVQM
                     KGCLAAGFPFTVGFSVYESLYDADGEPKAHVPLPGGDDGLLGGHAVLAVGYDDHTQAF
                     KLRNSWGVKAQEKGYFYLPYSYLTNSDLADDFWTIRLVDAPDA"
BASE COUNT          506 a          958 c          964 g          541 t
ORIGIN      
        1 ccacccagga aatatcgaga gacgagagat tccggaggtg cgcgtcatcg acgtggcgaa
       61 gagcgattcg ctcgacgacg cgaacgccac caccccgcgg cggacgttcg cggttgcggg
      121 aggaacggcc tgcttcggcg acagcggcgg ccccgcgctg tcgatgacga gtggcgcgtt
      181 ggtcggcgtt tattcacgca tcactggcga ttgtttcgcg gtcgaaagcc gcaacacctt
      241 catgcaagcg gcgagcttct ccgacttgtt cacacgggca ttcgagctcg tgggcgaaca
      301 gcccgcgctc gagccggggc cagaagcgcc cccgagttct ggcgcggcgg gagctccggc
      361 cacggcgccc gcgccgcaag acgggcgtca cgaagcattt cgatgcgcgg tagcggtgcc
      421 cggcgatcag tcgggttcga gcttcggcgc gacctcggta tttgcgctcg tgctcttctg
      481 gctcactcgt cgccgccgcg agaagagccc tttctaggcc ggctcatgcc gaagcggtcc
      541 atgcgcttca tcagcgtacg gcgcgagatg ttgagcagac gcgccgcctc ggtttgattt
      601 ccggcggtgc gctcgagcgc ttcgcgaata cgtcgctgtt cgcggcggtc gagctccgca
      661 cgaaagacag aggtgggcag ctcatccgcc gagaacctct ccgtttgctc gatttcggtc
      721 gcttccggct ttgccagcga ctcgggagga ggtgcctcgg ggggcgtcgt agagcgctgc
      781 aggttcagcg cgccgacttc gatcacgccg ccctcgctga tgagcagcgc gcgctcgatc
      841 acggatttga gctcgcgcac gtttcccggc caccgatagt cgcggagtgc atccagagcg
      901 gcggtgctga actccacgtg cccttcgcct gccttttgcg caaagtgccg cgccaaatct
      961 tcgacgtcgt caggtcgctc gcgcagcggc ggtatcgaaa tggtgaggcc gttcaaacga
     1021 tagaagagat cggctcgaaa cgaacccttc ttgacctgag ttgcgaggtc gcggttcgtt
     1081 gcggccacga tgcgcacatc gacgtgcacg ggtcgaactg cgccgaggcg caatacctcg
     1141 cccgtctcga gcacgcgcag aagcttggct tgcaccgacg cgggcagctc gccgatttcg
     1201 tccaaaaaga aggtcgtgcc gttggcctct tcgaacaagc cgcgcttggc cgcgacggca
     1261 cccgagaagg cgccgcgctc gtacccgaag agctcgcttt cgaccaaagt ctcgggaatc
     1321 gtggcgcaat tcaagctgac tagggcgcgg cgacggcgcg gcgattgtcg gtgaatcgcc
     1381 tgggccatga gctccttacc gctgccggtc tcacccatga tgagcacact caccgagctc
     1441 cgcgcgatct gttgagcgaa cgtgtacagt tcgcgcattc gctggtcgcg cacgaccgga
     1501 gcgctttcgc ctagatccgg aggttgcgtg gtcggcggtg gttcggaggg gagctgcacc
     1561 ggttgagcgc aacgcatgac cgccgaagtc tccggatgct cggcgcgtgt gatcagcatc
     1621 gagaaaccgc caatcgagac cacgctcccg ctcggcaacg gtttgggcgc gtcgacgacc
     1681 aggcgatgac catcgacgaa cgtcgcgttg cggcttccga cgtcgacgat gtagggcggg
     1741 tcggcagccg tgattacggc gtggcgttcg gagacccaag cgtgatcgag cacgatgctc
     1801 gcgctcttgc ggcggcccac gctgtattcc ttaccgggct cgagcgtcat gctgcgcggt
     1861 ggtcggccgg gcgcgtgaat cgtgagcacg tacgggccac tctcaggggc tggcggagct
     1921 tccgtcgtcc cgccgggaga acggtcgtcg gtgggacgaa tcatgagcca agtcgcccac
     1981 gcagttcgag agcggaggtc cttttcgcgc ggtctgaagc taccatcccc tcggtcatac
     2041 gtccctccgc gccccgcatt tagctccagc gctgaaacac tcgccgcgag tcgcaaggct
     2101 ccgttctcat cctgagtcgc aagcgcgtca tgaacgcgtt ggcggattct gccggtgtgc
     2161 gcggtgggag gcgcgagact cgatgccggc caccgctcga cccgcggcat cacgtgattt
     2221 ggaatgtcac cgtgatgctg cgatccgccg tggcctcgct cgtgccgcgc tgatgcgacc
     2281 aagtcacggt cgaagcgcct cgcttcagcg cggagatggt aaacacgcgt tcgcccccgc
     2341 ctcccggctt cagagagcgg ggacgaaacg tgtctgccac ttcgagcaac tcccgattgg
     2401 aaacggccac atgccagcga aagcccgtcg ttgggcgctc gccgaggacc aatgacctgc
     2461 tttcaccgac gctcagcgtt atcgtcgcgc catccgcgga ttgatccagg tctggcatgc
     2521 gcccgagctc atcacgcgtc gggggcatcc accaagcgaa tagtccagaa atcgtcggcc
     2581 aggtccgagt tggttagata ggagtaaggc aggtagaagt agcccttctc ctgcgccttc
     2641 acgccccacg agttccggag cttgaacgcc tgtgtgtggt cgtcatagcc caccgcgagc
     2701 acggcgtgac cgccgagcag cccgtcgtct cctcccggaa gcggcacgtg cgccttcggt
     2761 tcaccgtcgg catcgtaaag gctttcgtag acggaaaagc cgaccgtgaa agggaagccc
     2821 gccgccagac agcccttcat ctgcacgagg ctttgaggca cgcgactgta cgcgagcacc
     2881 cggtactgct ccgcctcgcc ataagcgtcg ttgctcggct tctgaactgc cttcgcgtcc
     2941 gaaggaaaca ggtccgtatc ggggtttgc
//
LOCUS       DASTKS010001142         5486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1969649, whole genome shotgun sequence.
ACCESSION   DASTKS010001142 DASTKS010000000
VERSION     DASTKS010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969649"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFQ35_24235"
     CDS             <1..233
                     /locus_tag="VFQ35_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9933844.1"
                     /translation="LARSAPEPVSSTAESSGMVAELSPKELEDRQRILEALTQCSGNQ
                     SKAAEKLGISRSTLINRLDAYRIGRPRKRPGG"
     gene            complement(250..552)
                     /locus_tag="VFQ35_24240"
     CDS             complement(250..552)
                     /locus_tag="VFQ35_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9933845.1"
                     /translation="MTLTITGLIILLIIAGVCGAIGRAIGGGTGGGFFVSIAIGFVGA
                     LLGSFLAAHFHLPELLTVMVEGRAFPILWSIIGASLFVALVHLVSGGVGSRRYRYY"
     gene            complement(668..2590)
                     /locus_tag="VFQ35_24245"
     CDS             complement(668..2590)
                     /locus_tag="VFQ35_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9933846.1"
                     /translation="MLGLINLGKSFGARNLFEGVSLNLVPGARYGLVGANGSGKTTLL
                     KILAGDEPSDGQVTFAQNLRMGVLRQDRFLDDAQPILDVAKAGDEVVSGALAEQAALI
                     ASGVVDPVRLAELEDRIAQHDGYTLEARASAILEGLGIPVAQHRMPLATLSGGFKLRV
                     LLAQVLIGGPDVLLLDEPTNHLDILSIRWLETFLASYQGLAVVISHDQRFLDNVVTHV
                     LDVDYGTITLYTGNYSAFVVQKRETRARKEAEIARAEAVIAEKRAFVERFGAKATKAR
                     QAQSRLKQIERIEVEELEQSSRRAPLFRFEEARPSGRDVLELSGISKSYGAKRVLTDV
                     SLSVRRKERVAIIGPNGLGKSTLLKIATGNLEPDAGKVTWGYEARVGYFAQDHREVLD
                     DAEATPLDVIGDAIPAEPPNVVRGELGRVLFSGDDVKKPVAALSGGEAARLVFCRLSV
                     QRPNVLVLDEPTNHLDLESIQALTEALLAYPGTLLFVSHDRAFVSALATRVLEVTEKG
                     FQDFPGTYQEYLERSGDDHLDVAAVVLRDKRQRENLESATEAANRTASWEEKKRKNNR
                     QKQLPALRDRALEAISAAEARKAEITAMWCEPGFFERTPPERVAALQAEEQELGPRIE
                     RLIAEWEALEAELGAI"
     gene            complement(2606..3580)
                     /gene="hflC"
                     /locus_tag="VFQ35_24250"
     CDS             complement(2606..3580)
                     /gene="hflC"
                     /locus_tag="VFQ35_24250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET9933847.1"
                     /translation="MSRLRIGLLFWVLVVAGAITASSSFYTLQETEQAIITEFGKPVG
                     QPVTQAGLHFKVPITQEVNVIEKQILEWDGQRSEMPTRDKLYIAVDMFARWRITRPLE
                     YFRRLRDERSAQSRLDDILGSETRNSIAKHDLIEVIRTTKDRKPLRDETLSAASEGYG
                     RIGVLQPIQKGRSAIEQEIRDAAKTKLAELGIDLLDVRFKRINYNPSVVEKIYERMIS
                     ERQQIASLFRSEGEGEAAKIMGDRERDLSVIESEAYKSVQQIRGEADAKVTSIYAGAY
                     SQSAEARSLYAFVKTLATYKKVVSENDTLVLSTHSELYRFLNDVGAVK"
     gene            complement(3577..4569)
                     /gene="hflK"
                     /locus_tag="VFQ35_24255"
     CDS             complement(3577..4569)
                     /gene="hflK"
                     /locus_tag="VFQ35_24255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET9933848.1"
                     /translation="MATAKSPTRPRGPRITLRTITTALLVLGALFFVWTGFYTVPAEA
                     EGVLLRFGRFNSVVPSGLHFKLPLGIDIAHVVPVKRQLKVEFGFGTEGGSSPAQVGPQ
                     NEWPEVKSMVTGDLNAVLVEWVIQYRIQHPDQYLFNVRNPDETLRDASESVMREVVGD
                     RTVDEVITIGRQEVEDEALQKLRALVTAYHMGIKIDQVQLKNVNPPEKVQASFNEVNQ
                     AQQERERAINIANGEYNKEVPRARGRAAQSIRAAEGYAKQRVNEAEGEVSAFNALLAE
                     YNKAPEVTRRRLYLETMSEILPKVKNKVIVGDDVKGLLPLLNLNALAEAGGKGK"
     gene            complement(4701..>5486)
                     /locus_tag="VFQ35_24260"
     CDS             complement(4701..>5486)
                     /locus_tag="VFQ35_24260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9933849.1"
                     /translation="LVRAISNLVINAVDAIEGAGRIDIRVRGRVLQERREAADPIPPG
                     DYAVIEVQDSGTGIPADRLQRIFEPFFSVKRGRGSPSTGLGLAIVRRIVQDCYGFVQV
                     RSQVGLGTTFSLYFPKQPGAALPETKALHAVRGGNERILVVDDESLQLRVARRILGKL
                     GYTVVTSNSGDAALELYSSTESAERFDLVIVDMMMPGTLNGIATIEQMRKLRPEQKAL
                     VATGFAPERMETLASERGLAWLAKPYTLSGLSEAVRNVLNSAV"
BASE COUNT          932 a         1806 c         1727 g         1021 t
ORIGIN      
        1 agctcgcccg ctccgcgccc gaacccgtga gttcgacagc cgagagctcg ggaatggtgg
       61 ccgagctttc gccaaaagag ctagaggacc gccagcgcat tttggaggca ctcacccagt
      121 gctccgggaa ccaatccaaa gccgccgaga agctcggcat ctcccggagc acgctgataa
      181 accgactgga tgcgtaccgc atcgggcgcc ctagaaagcg cccgggaggc tgacgaaccc
      241 gcccagcatt caatagtacc gatagcggcg gctgccgacg ccacccgata cgaggtgaac
      301 gagcgcgacg aagagtgacg cgccgatgat ggaccacagg atcgggaacg ctcgcccttc
      361 caccatgacc gtcaggagct ccggcaggtg aaagtgcgcg gcaaggaagg agccgagcag
      421 cgcgccgacg aagccgattg cgatcgagac gaagaagccg ccgcccgttc cgccaccgat
      481 cgctcgaccg atggcgccgc aaacgccggc gatgatcaga agaatgatca gacccgtgat
      541 tgtgagcgtc atgacgaagc tcgttgcaag cgctgggccg cgctgttcag cggcagatca
      601 gctagatgcg gcggattcgt gcggcgattt gccccacgcg cgcacgggcg ccacgagcgc
      661 gagtcatcta gatcgcgccg agctccgcct cgagcgcctc ccattcggcg atcaagcgct
      721 cgatgcgcgg cccaagctcc tgctcttccg cttgcagcgc ggcgactcgc tccggcggcg
      781 tgcgctcgaa gaatcccggc tcacaccaca tcgccgtgat ttccgccttc cgcgcctcgg
      841 ccgcgctgat cgcttctaga gcgcgatcgc gcagcgcggg gagctgcttt tggcggttgt
      901 tcttgcgctt cttctcctcc cacgacgcgg tgcggttggc ggcttcggta gcgctctcga
      961 ggttctcccg ttgccgcttg tcgcgcagta ccaccgccgc gacgtcgagg tgatcgtcac
     1021 ccgagcgctc gaggtactcc tggtaagtcc cggggaagtc ttgaaaacct ttctcggtga
     1081 cctcgagcac gcgcgtcgcc agcgcggaca cgaatgcgcg gtcgtgcgag acgaacaaga
     1141 gtgtcccggg gtacgcgagc aacgcctcgg tcaacgcctg aatggactcg agatccaggt
     1201 gattcgtcgg ctcgtccagc accaacacgt tgggccgctg cacgctgaga cggcagaaga
     1261 ctaggcgcgc cgcttctcca cccgagagcg ccgccaccgg cttcttcacg tcatcccccg
     1321 agaagaggac gcgcccgagc tcaccgcgca ccacgttcgg cggctcggcc gggatggcgt
     1381 caccgatcac gtcgagcggc gttgcctcgg cgtcgtcgag cacttcacga tgatcctgcg
     1441 cgaagtagcc gaccctcgcc tcgtatcccc acgtgacttt accggcgtct ggctccaggt
     1501 tccccgtcgc aatcttcagg agcgtcgact ttcccaaccc gttcggaccg atgatggcga
     1561 cgcgctcctt gcgccgcaca ctcagggaga cgtcggtgag cacccgcttc gcaccataag
     1621 acttcgagat cccgctgagc tcgagcacat cgcgcccgct cggtcgcgct tcttcgaagc
     1681 ggaacagcgg cgcgcgcctc gagctctgtt cgagctcctc gacttcgatg cgctcgattt
     1741 gcttcagccg gctctgcgct tgccgtgcct tggtcgcctt cgccccgaag cgttcgacga
     1801 acgcgcgctt ctccgcgatg acggcttcgg cgcgggcgat ctcggcctct ttgcgcgcgc
     1861 gggtctcacg tttttgtacg acgaaggcgc tatagttccc ggtgtacagc gtgatcgtgc
     1921 cgtaatcgac gtcgagcacg tgggtgacca cgttgtcgag aaaacgctgg tcgtgcgaga
     1981 tgacgacggc gagcccctgg tagctcgcga ggaaggtctc gagccagcga atcgacagga
     2041 tgtcgaggtg gttcgtcggc tcatcgagca agagcacgtc ggggccgccg atcagcactt
     2101 gcgccaacaa gacccgcagc ttgaagccgc ccgacagcgt ggcgagcggc atgcgatgct
     2161 gcgcgacggg gatgccgagg ccttcgagga tcgccgatgc ccgcgcttcg agcgtgtacc
     2221 cgtcgtgctg cgcgatgcga tcctcgagct cggcgaggcg caccgggtcg acgacgcccg
     2281 aggcgatgag cgcggcctgc tcggcgagag caccgctcac cacctcgtcg ccggccttgg
     2341 cgacgtcgag gattggctgc gcgtcgtcga gaaatcggtc ctgacgcagc actcccatgc
     2401 gcaggttctg cgcgaacgtc acctgcccat cgctcggctc gtcgccagcg aggatcttca
     2461 gcagcgtcgt ttttccggag ccgttggcgc cgaccaagcc gtagcgagcg cccgggacca
     2521 agttgagcga gacgccttcg aagaggttcc gcgcgccaaa ggattttccg aggttgatca
     2581 gccctaacat cggcaggtca cggcgctact tgacggcgcc gacgtcgttc aggaagcggt
     2641 agagctcgct gtgagtcgag agcaccaagg tgtcgttctc cgacaccacc ttcttgtagg
     2701 tcgcgagcgt cttgacgaag gcgtagaggc ttcgcgcctc cgcgctctgc gagtacgcac
     2761 ccgcgtagat gctggtgacc ttggcgtcgg cctcgccgcg aatctgctgc acgctcttgt
     2821 aggcctccga ctcgatcacg ctgagatcgc gctcacggtc gcccatgatc ttggccgctt
     2881 cgccctcacc ttccgagcgg aagagcgagg cgatttgctg ccgctcggag atcattcgct
     2941 cgtaaatctt ctcgaccacg ctggggttat agttgatgcg cttgaaccgg acgtcgagga
     3001 gatcgatacc gagctcggcg agcttggtct tcgccgcgtc gcggatctcc tgctcgatgg
     3061 cggaacggcc cttttggatg ggttggagta ccccgatgcg gccgtagcct tcactcgccg
     3121 cgctgagggt ctcgtcgcgc agcggcttgc gatctttggt ggtccgaatg acctcgatca
     3181 agtcgtgctt tgcaatcgag ttacgcgtct cactgccaag gatgtcgtcc aggcgagact
     3241 gggcgctgcg ttcgtcgcgc agacggcgga aatattcgag cggccgcgtg atgcgccagc
     3301 gcgcgaacat gtccaccgcg atgtagagct tgtcgcgcgt cggcatctcg ctgcgctgac
     3361 cgtcccactc gagaatttgc ttctcgatga cgttcacttc ttgcgtgatc ggcaccttga
     3421 agtgaaggcc cgcctgggtg acgggttgcc ccaccggctt tccgaactcg gtgatgatgg
     3481 cctgttcggt ttcctgcaga gtgtagaagg aagaagatgc ggtgatggca cccgccacga
     3541 cgagcaccca aaacaagaga ccaatgcgta agcggctcat ttgcccttcc ctcccgcctc
     3601 ggcgagcgca ttcagattga ggagcggcaa gagtcccttc acgtcgtctc ctacgatgac
     3661 cttgttctta accttgggga ggatctcgct catggtttcg aggtagagcc gccggcgcgt
     3721 cacctcggga gccttgttgt attccgccaa cagggcgttg aacgccgaca cctcgccctc
     3781 cgcctcgttc acgcgctgct tggcgtagcc ctccgcggcc cggatgctct gcgcggcgcg
     3841 tccgcgagcg cgcggaacct ctttgttgta ctcgccgttc gcgatgttga tcgcccgctc
     3901 gcgctcttgc tgggcctggt tcacctcgtt gaacgacgcc tggaccttct cgggggggtt
     3961 cacgttcttg agctgcacct ggtcgatctt gatccccatg tgataggccg taacgagggc
     4021 gcgcagcttt tgcagcgcct cgtcttccac ttcttgccgg ccgatcgtga tcacctcgtc
     4081 gacggtgcga tcgccgacga cctcgcgcat cacgctctcg gacgcatcac gcaacgtctc
     4141 gtcgggattt cgaacgttga acaagtattg gtccgggtgc tggatgcgat actgaatcac
     4201 ccactcgacg agcacggcgt tcaagtcgcc ggtcaccatc gacttgacct cgggccactc
     4261 gttttgaggc ccgacctgcg cgggcgagct cccgccctcg gtgccgaagc cgaactccac
     4321 cttcagctgt cgcttgaccg gcaccacgtg cgcaatgtcg ataccgagcg ggagcttgaa
     4381 gtggaggccc gacggcacca cgctgttgaa ccggccgaag cgcaacaaca cgccctcggc
     4441 ctccgccggc acggtgtaaa acccggtcca cacgaagaag agcgcgccca acacgagcag
     4501 cgcggtcgtg atggtgcgta gagtgattct cggaccgcga ggtcgcgtag gagacttggc
     4561 tgttgccatg gctttctttg gctccgcatc ggcaaggcgg aatcgcaggg catgttgcgc
     4621 tgcgcgctcg cgaagtcaaa tggacttcgg acctgatcga cgcgacagag cccgaattgg
     4681 ctccggcctc cgatcgtcgt tcacacggca gagttcagga cgttgcggac cgcctcgctc
     4741 aagccgctca gcgtgtaggg cttggcgagc catgcgaggc cgcgctcgct ggccagcgtc
     4801 tccatgcgct cgggggcgaa accggtcgcc acgagcgcct tttgctcggg ccgtagcttc
     4861 cgcatctgct cgatcgtggc gatgccgttg agcgtgcctg gcatcatcat gtcgacgatc
     4921 acgaggtcga agcgctcggc cgattcagtg ctcgagtaga gctcgagcgc cgcgtctccg
     4981 gagttcgacg ttacgaccgt gtaaccgagc tttcccaaga tgcgccgagc aacgcgaagc
     5041 tgcagcgatt cgtcgtcgac caccaggatt cgctcgttgc caccccggac cgcgtgcaac
     5101 gccttggttt cgggcagcgc cgcccccggt tgcttcggaa agtacagcga gaaggtggtg
     5161 ccgagaccga cttgactcct gacctggacg aagccgtagc agtcctgcac gatgcgccgc
     5221 acgatggcaa gtccgaggcc ggtgctcggc gacccgcgac ctcgcttcac gctgaagaac
     5281 ggctcgaaga tccgctggag acggtccgcg ggaattccag tgccggagtc ctgaacctcg
     5341 atgaccgcgt agtcgccggg cgggattggg tcggccgcct cgcgccgctc ctgcaagacg
     5401 cgccctcgga ctcggatgtc gatgcgaccg gcaccttcga tggcgtccac cgcgttgatc
     5461 accaagttcg agatggcccg cacgag
//
LOCUS       DASTKS010001143         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_627119, whole genome shotgun sequence.
ACCESSION   DASTKS010001143 DASTKS010000000
VERSION     DASTKS010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627119"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFQ35_24265"
     CDS             <1..841
                     /locus_tag="VFQ35_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933850.1"
                     /translation="ALAYVEKEGSDVRLVAAEGDEHDVLKTVGLGSSGAPARGQRGAL
                     SLSFDPREANQVNLLGRVGESECVDSGRRECSGFSWWRLVGGRAESQGFSLSVPAPCT
                     EHAVSLLGAPGKLAYSVCSDQAGAERRTTLFTVQTDPQYARADPVLPGCQPLGLFGEG
                     SSVWLIGECEGKRRAARAGADNGPVEVRDVASPRVECVGGKARLRAGPIEVELNAPRA
                     RLELVLPTVLAPRGSRAVWSGEALLVAAAPLRKLSLARYACEGNQLKEVSVGAASAAP
                     SAK"
     gene            849..>2422
                     /locus_tag="VFQ35_24270"
     CDS             849..>2422
                     /locus_tag="VFQ35_24270"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9933851.1"
                     /translation="MGHVPLLDELAIVAALGVVVTVLLSKLKLPTVAGLLAAGALLGP
                     YGLGLATSVHSIEVLAEVGVVLLLFSIGLEFSLTRLRDIFRQVALGGVLQVGLTTVVT
                     AAIAIALGTSISRGVFYGFAFALSSTAIVLRALTERRELDAPHGRFIVGTLIFQDLCV
                     VPMVLIVPLLATGSGAASAATSIGLALLKAGAVVVGTVAVARLVVPKLLGWVDASRSR
                     EVFLLAILGLCIGTAWLTAQAGLSLALGAFLGGMVVADTEYGHRAMGDILPLRDAFVS
                     IFFVSLGMLFNARVVLEHPILVSALLAGFLVAKGALATLAALAMRFPARVAWLAGVGL
                     AQFGEFGFVLTRLAQSSGVIDEASAAPLLAAGIGSMFLTPLLVRAAPHVTAGERLLGP
                     LERLIGVRGIDEVDEEDAAHSRISGHVVIVGFGLAGKFAARVLQETRTPFVVLELNAD
                     SVRRGKESGLPVYYADATSEEALRHAHVEHARLVVLLMNDPQAAARVVDTVRRVAPSV
                     PILMRTRYMLEREALFA"
BASE COUNT          328 a          797 c          831 g          466 t
ORIGIN      
        1 cgcactggcg tacgtcgaga aagaaggaag cgacgttcgg ctcgtggccg cggagggtga
       61 cgagcacgac gttttgaaga ccgttgggct cggatcgagc ggcgctccag cgcgcggaca
      121 gcgcggcgca ctttcattga gtttcgaccc gcgagaagca aaccaggtaa acctcctcgg
      181 tcgtgtcggc gagagcgagt gcgtcgactc ggggcgccgc gaatgcagcg ggttttcgtg
      241 gtggcgcctc gttggtggac gcgccgaaag tcaggggttc tcgttgtcgg tgccagcgcc
      301 ttgcaccgag cacgcagtga gcctgctcgg cgccccggga aagctcgcgt actcggtttg
      361 ctcggaccaa gcaggggccg aacgacgcac gaccctgttc acggtccaga cggacccaca
      421 atacgcgaga gcggacccgg tgctacccgg atgtcagccg ctcggcttgt tcggcgaggg
      481 cagctcggtc tggctcatcg gcgagtgcga aggcaagcgc cgcgctgcgc gcgctggcgc
      541 cgacaatggg ccggtcgaag tgcgcgacgt cgcttcgccg cgcgtcgagt gtgtaggagg
      601 gaaggcgcgc cttcgcgccg ggccaatcga agtcgagctc aacgctccgc gagcgcggct
      661 cgagctggtt ctgcccaccg ttctcgcgcc gcgcggcagc cgagcggtgt ggagcggtga
      721 ggcgcttttg gtggccgccg cccctctgcg caagctatcg cttgctcgct atgcgtgcga
      781 gggtaaccag ctgaaagagg tctcagtggg cgcagcgtcg gccgcgcctt cggcaaagta
      841 gttcactcat gggtcatgtc cccctcctcg acgagctcgc aatcgtggct gccctcggcg
      901 tcgtcgtcac ggtgctgcta tcgaagctca agctccctac cgtggctggc ttgctcgccg
      961 caggcgcgct gctcgggccc tacgggctcg gcctcgcgac ctccgtgcat tcgatcgaag
     1021 tgctcgcgga ggtcggtgtc gtgcttctct tgttttccat cggcctcgag ttctcgctca
     1081 ctcggctgcg cgacatcttc cggcaagtag ccctcggcgg agtgctgcaa gtcgggctca
     1141 cgacggtcgt cacggccgcg atcgcgatcg cgctcgggac gtcgatctct cgcggcgtgt
     1201 tctacggttt cgctttcgcc ctgtcgagca ccgcgatcgt gttgcgcgcg ctgaccgagc
     1261 gccgggagct cgacgcgcca catgggcgct tcatcgtcgg taccctcatc tttcaagatc
     1321 tgtgcgtcgt cccgatggtg ctgattgtac cattgctcgc caccggctca ggcgccgcga
     1381 gcgccgcgac cagcattggg ctcgcgttgt tgaaagcagg ggccgtcgtc gtcggaacgg
     1441 tcgcggtggc gcggctcgtc gtgccgaagc tgctcggttg ggtcgatgcg agtcgcagcc
     1501 gcgaagtgtt tttgctcgcg atcctggggc tttgcatcgg gactgcgtgg cttacggccc
     1561 aggcagggct ttcgctcgcg ctcggcgcct ttttgggcgg catggtcgtg gccgacacgg
     1621 agtacggcca ccgggcgatg ggcgacatct tgccacttcg cgacgccttc gtgagtatct
     1681 tcttcgtatc gctcggcatg ttgttcaacg cccgagtggt gctcgagcat cccatcttgg
     1741 tgtcagcgct gctcgccggc ttcctggtcg ccaagggcgc gctcgcgacg ctcgccgcgc
     1801 tcgcgatgcg cttccccgcg cgcgttgcgt ggctcgccgg cgtggggctc gcgcagttcg
     1861 gcgagttcgg cttcgttctc acccgcctcg cgcagtccag cggggtcatc gacgaggcct
     1921 cggccgcacc gttgctcgcg gctggcatcg gctcgatgtt cctaacgccc ctgctcgtcc
     1981 gcgccgcgcc gcacgtcacg gccggagagc gtttgctcgg cccgctcgag cgcttgatcg
     2041 gcgttcgcgg catcgacgaa gtcgacgagg aggacgcagc gcactcgcgc atcagcggcc
     2101 acgtggtcat cgtcggtttc gggctggcag ggaagttcgc tgctcgcgtc ttgcaagaga
     2161 cccgcacgcc gttcgtcgtg ctcgagctca acgcggactc ggtcaggcgt ggcaaagagt
     2221 ccgggttacc cgtctactac gccgacgcca cgagcgaaga agcgctccgt cacgcccacg
     2281 tcgaacatgc gcgcctagtg gtgctgctga tgaacgatcc tcaagcggcg gcgcgcgtgg
     2341 tcgatacggt tcgccgcgtc gccccgagcg tccccatctt gatgcgcaca cgctacatgc
     2401 tggagcgcga ggcgctgttc gc
//
LOCUS       DASTKS010001144         4516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_181501, whole genome shotgun sequence.
ACCESSION   DASTKS010001144 DASTKS010000000
VERSION     DASTKS010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181501"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFQ35_24275"
     CDS             complement(<1..257)
                     /locus_tag="VFQ35_24275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933852.1"
                     /translation="MLLSATFSYAQAAPAKYRSHAYYAAHGAEIAALLAGSYLLDAHG
                     AQSPGADPTWFPGDFAFRAHRNDRANAVSDVLVAFTLTLPPA"
     gene            complement(351..1445)
                     /locus_tag="VFQ35_24280"
     CDS             complement(351..1445)
                     /locus_tag="VFQ35_24280"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9933853.1"
                     /translation="MTSSLKTALRLLVGTLAFGAAIGCGGGPQYGAPATGGATTGVVP
                     PNDSGPGDDMPITEAPKPPKATGNPFEGVKLWVDPESNAMLRSKALMASQPENAKLIH
                     DKIAVYSQALWLGEWNSNVYRKVRYVVDLAAQDKSLPIFVAYNLPGRDCGNESAGGLK
                     TGDMYKRWIRKIAAGIGDRAAVVVLEPDALGLLEKADAKDPSKPCLTKEQQEERLALL
                     HDAVKVLRQNPKTIVYIDGAHSKWDPVDKIADRLQRAGVEDAHGFAINTSNYRANEEL
                     LPYGKAVSAKLGGAHFVVDTSRNGNGPLGVEWCNPKGRKLGLPPNTNTGDPLIDAYLW
                     LKRPGESDGACNGGPQAGAFWDEQAIELAK"
     gene            complement(1611..2288)
                     /locus_tag="VFQ35_24285"
     CDS             complement(1611..2288)
                     /locus_tag="VFQ35_24285"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9933854.1"
                     /translation="MDDERLELALLSDETLAALIAHGTPRAPLLELARRVYHEALELL
                     ASEQRQAALLRSIELAEAAIAEVEAQRRRLKVEQPRIACARGCASCCILRVEIGALEA
                     ERLRPVVEALELSAAVEALASEVGSLSRLERLEARRSCALLGRDGECRVHAVRPMACR
                     AANSMSSDACRRAAQLGDSSVGIPVEGTSFGLMRSAALGLSLACADAGLDASQTELHT
                     ALRRGIR"
     gene            complement(2324..4189)
                     /locus_tag="VFQ35_24290"
     CDS             complement(2324..4189)
                     /locus_tag="VFQ35_24290"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family metalloprotease"
                     /protein_id="HET9933855.1"
                     /translation="MQRTESPEVAKLCSSRKRPFVGISSVFALGVIVGCSAGTVDDPS
                     GGASAKGGGNANGGANENNTGPTGGSDAHGGTSSASGGAPNGSGGARQSGGSSSGGSA
                     GGSQATGGTQNGTSTGGRSAGGSSSGGQGAGGRMTGTGGTSSGGSSSRGGAPNGGSGN
                     VAGSSPSGDDGCGSAANVTLSQIAVYQTVKIPIMQSGTEVAVASRKTDVVAGKDAMFR
                     LFVTPDTGFTAHAMSGRIVVDNGGTIDTYYSKVNISGASAEGSLTNTFQVTVPKDKIT
                     RTTQYYAEIAECGTMTGTGQSARFPASGSTAVGARETGTLKIKVIPLSANSMVADTSA
                     TALNVYKAAFMAMYPITSIDITVGDTLTVSDPKDWTSMLDAVRSKRQMDQPPADVYYY
                     GFLKPTNTLQQYCGNGCTAGIGYVVPSGSSGQVASQRAALGLAYADTTSAQTMVHEVG
                     HNHGRNHAPCVQGGTINGVDANYPYSNGATGVYGWDNRNSTLLDPNRTDIMGYCNNKW
                     ISDYTYDGILTRVATVNGAPMSVLVPAELIHPWRVLLVDSRGSRWGAPIDQAVAPAGQ
                     AELADVLDATGNHITTTTVYRTEIADLDAFSFEVPPPEAGWAAIQVKGGPALSYP"
BASE COUNT          683 a         1451 c         1560 g          822 t
ORIGIN      
        1 gccggcggta gggtgagggt gaaggcgacg agcacgtcgc tcacggcgtt ggcgcggtcg
       61 ttgcggtgcg cgcgaaacgc gaagtcgccc gggaaccacg ttggatcggc gccaggcgac
      121 tgggctccgt gcgcatcgag caagtagctc ccggcgagca gcgcggcgat ctcggcgccg
      181 tgcgcggcgt agtaggcatg ggagcggtac ttcgcgggag cagcttgtgc gtacgagaac
      241 gtcgcgctga gcaacatggc agcgcacgtg agcgcccaca aagcaatcga ccccgcatga
      301 gcgctgtgtc tcacgcgggg tcgatcggcc gaagtcacgt gcgtgacggc ttactttgcg
      361 agctcgatgg cttgctcgtc ccagaacgca ccggcttgcg gcccgccgtt gcaggcgccg
      421 tccgactcac ccgggcgctt gagccagagg taagcgtcga tgagcggatc gcccgtgttc
      481 gtgttcgggg gcagaccgag cttgcggccc ttcgggttgc accactcgac gccgagcggg
      541 ccgttgccgt tgcggctcgt gtcgaccacg aagtgagcgc cgccgagctt ggcgctgacg
      601 gccttgccgt agggaagaag ctcttcgttc gcgcggtagt tcgaggtgtt gatggcgaag
      661 ccgtgcgcgt cctcgacacc ggcgcgctgc aaacgatcgg cgatcttgtc gactggatcc
      721 cacttggagt gagcgccgtc gatgtagacg atggtcttgg ggttttggcg cagtaccttc
      781 accgcgtcgt gcagcagggc gaggcgctct tcttgctgct ctttggtgag gcagggcttg
      841 ctgggatctt tggcgtccgc cttctcgagc aggccgagcg cgtcgggctc gaggaccacg
      901 acggccgcgc gatcaccgat gccggccgcg atcttgcgga tccagcgctt gtacatgtcg
      961 ccggtcttga ggccacccgc cgactcgttg ccgcagtcac gaccgggcag gttgtacgcg
     1021 acgaagatgg ggagggattt gtcctgggcc gcgagatcca ccacgtagcg gaccttgcga
     1081 tacacgttgc tgttccactc accgagccag agcgcctgcg agtagacggc gatcttgtcg
     1141 tggatgagct ttgcgttctc gggctgcgac gccatcagcg ccttggagcg cagcatcgcg
     1201 ttcgactccg gatcgaccca gagcttcacg ccttcgaagg ggtttccggt cgccttggga
     1261 ggcttggggg cctcggtgat cggcatgtcg tcgccggggc cgctatcgtt cgggggaacg
     1321 acgccggtgg tcgctccacc ggtcgcgggc gcaccgtatt gcgggccgcc gccacagccg
     1381 atggctgcac cgaacgcgag ggtaccaacg agaaggcgaa gagcagtctt gagagaggag
     1441 gtcattgcga gtctcgatga ggacggagct accattctgg ggtctctccg atatgcctaa
     1501 atctcggcgt ttgcaaccag atagaccagg cgggccgcag ggaatgacgg tcttttcggc
     1561 ggctgagtga aaaagccagc cgctttgagc caaaacgacg attgggtttg ttaacgaatt
     1621 ccgcgccgca gcgcggtgtg gagctcggtc tgagacgcgt cgagtccggc gtctgcgcag
     1681 gcgagggaga gcccgagcgc ggccgagcgc atcaagccga acgaggtgcc ttcgaccggg
     1741 atgccgacgc tcgagtcgcc gagctgtgcg gcgcgacgac aggcgtcgct gctcatcgag
     1801 tttgcggcgc gacacgccat cggtcgaacc gcgtgcaccc ggcattcgcc gtcacgcccg
     1861 agaagcgcgc aggagcggcg tgcttcgagt cgctcgaggc gcgatagcga gccgacctcg
     1921 ctcgccagcg cctcgaccgc tgcggagagc tcgagcgcct cgaccacggg acgcaaacgc
     1981 tccgcctcga gggcaccgat ctcgactcgc aggatgcaac agctcgcgca gccccgcgcg
     2041 caggcgatgc gcggctgctc taccttgagc cgccggcgtt gcgcttcgac ttcggcgatg
     2101 gcggcctcgg cgagctcgat gctccgaagg agcgccgcct gtcgctgttc cgacgcgagg
     2161 agctcgagcg cctcgtggta cacgcgccga gccagctcga ggaggggcgc gcgcggcgtg
     2221 ccgtgagcga tgagcgccgc gagcgtctcg tcgctcagga gcgctagctc gagtcgctcg
     2281 tcatccatcg agcgtcaggc tagcgctcgt gcgcggcgcg gcgctacgga tacgaaaggg
     2341 ccggcccacc cttcacttga atcgccgccc agccagcttc cggaggcggt acttcgaacg
     2401 aaaaggcgtc cagatccgca atttcggtgc ggtacaccgt cgtcgtcgtg atgtgattac
     2461 ccgtcgcgtc gaggacgtcg gcgagttccg cctgaccggc gggtgcaact gcctggtcga
     2521 tgggcgcgcc ccaacgcgag ccccgggagt cgaccaagag cacgcgccag gggtgaatga
     2581 gctcggccgg aaccagcacg ctcatcggcg cgccgttcac cgtagcgacg cgcgtgagga
     2641 taccgtcgta cgtgtagtcg ctgatccact tgttgttgca gtaacccatg atgtcagtgc
     2701 gattcgggtc cagcaacgtc gagttacggt tgtcccatcc gtacacgccc gtcgcgccgt
     2761 tcgagtacgg gtagtttgcg tcgactccgt tgatggtgcc gccctgcacg cacggcgcgt
     2821 ggttgcgacc gtggttgtgc ccgacctcgt gcaccatggt ttgcgccgag gtcgtgtccg
     2881 cgtaggcaag cccgagcgcg gcgcgttgag acgcgacctg cccggagctg ccggaaggta
     2941 cgacgtagcc gatgccggcg gtacagccgt tgccgcagta ctgttgcaga gtgttcgtgg
     3001 gcttcaaaaa gccgtagtag tagacgtccg cgggcggctg gtccatttgc cgcttgctgc
     3061 gcacggcgtc gagcatggag gtccagtctt tgggatcgct caccgtcagc gtgtcgccca
     3121 ccgtgatgtc gatggaggtg atggggtaca tcgccatgaa cgccgccttg tagacgttga
     3181 gcgccgtagc ggacgtgtcg gccaccatcg agtttgcgct gagcgggatg actttgatct
     3241 tgagggtgcc ggtctcgcga gcgccgacgg cggtgctgcc gctcgcgggg aaacgcgcgg
     3301 actgtcccgt tcccgtcatc gtgccgcact cggcgatttc agcgtagtac tgcgtcgtgc
     3361 gcgtgatctt gtctttcggc accgtcactt ggaaggtgtt ggtgagcgag ccctcggcgg
     3421 atgcgccgga gatgttcacc ttcgagtagt acgtgtcgat cgtgccgccg ttgtcgacca
     3481 cgatgcggcc ggacatcgcg tgcgcggtga agccggtgtc gggcgtgacg aagagccgga
     3541 acatcgcgtc tttgccggcg acgacgtccg tctttcggct cgcaaccgcg acctccgtgc
     3601 ccgactgcat gatcgggatc ttcaccgtct ggtacacggc gatctgactc agtgtgacgt
     3661 tcgcggcgct cccgcagcca tcgtcaccgc tcgggctgct gccggccacg ttgccggagc
     3721 cgccgttcgg cgcgccgccg cgcgaactcg aacccccgga gctcgtcccg cccgtgccgg
     3781 tcatgcgacc gcccgcgccc tggccgccgg agctgctgcc gccggctgag cgcccgcccg
     3841 tggacgtacc gttctgagtg ccgccggtcg cttggctgcc accggcgctg ccgccactcg
     3901 aggagccgcc cgattgccga gcgcccccgg agccgttcgg ggcgccgccg ctcgcggagc
     3961 tcgtgcctcc gtgtgcgtcg gagccgcccg tcgggcccgt gttgttctcg ttggccccgc
     4021 cgtttgcgtt gccgcctccc ttcgcgctgg cgccccccga ggggtcgtcc accgtgccag
     4081 cgctgcagcc tacgatgacg ccaagggcga aaaccgagct gatgccgacg aacggcctct
     4141 tgcgagacga gcacagcttg gcgacttcgg ggctctccgt tcgttgcatt ccagatacct
     4201 cagctaccac gatcccaaac ggagtatgcg cgctccgctt tacgggtcgc agggactccc
     4261 gagcgtagcg cgcgatcgcc gtttgggaaa accacaactc aagatgccgt aacttcgaca
     4321 taccgctctt aattcgcctg tgaggaaagc tccggcctca gcgcatcctt caagggacga
     4381 tatttcttct gagggtggcg ggccctcgca ttccgcgcac gctggcaaat ttctcgggtt
     4441 ggcgcgtcgc gctcccgcga gccttggtgc gctacattgt gccgtgcgag caccaggtgg
     4501 tcgatcgggt gctcgg
//
LOCUS       DASTKS010001145         4821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1970005, whole genome shotgun sequence.
ACCESSION   DASTKS010001145 DASTKS010000000
VERSION     DASTKS010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970005"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1177)
                     /locus_tag="VFQ35_24295"
     CDS             complement(227..1177)
                     /locus_tag="VFQ35_24295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9933856.1"
                     /translation="MTDFERLKGARVLVTGGAGFIGSHLVDALLERGAKVRVLDNLAT
                     GKLENLAHVKERIEFFQDDIRDLAACQRACEAVDFILHEAALGSVPRSIEKPSDSIAV
                     NVSGTCNVFTAARDQRVKRIVYASSSSVYGTSEKLPKREGEEGLPLSPYALSKRMDEE
                     LTDIYARCYGMEIIGLRYFNVYGPRQDPHGAYAAVIPRFFAACRAGQAPVIYGDGQQS
                     RDFTYVGDVVAANLAALLGSGHSGSAFNVGAGRRTTVTDLARAIAKATGFNGSAEHKD
                     KRAGDVMHSIADTTRIFEAFGWRATTDLDRGIELCAAYYR"
     gene            1423..2280
                     /locus_tag="VFQ35_24300"
     CDS             1423..2280
                     /locus_tag="VFQ35_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933857.1"
                     /translation="MAAEPQQDPSPPQNGASMKAPPGAAPHILVEHLERRFDDFVALH
                     DINMSIGRGHVAVIIGGSGAGKTTLLKILIGLDRPTGGSVLLEGVDMTKLNDRELNRL
                     RRKLGMVFQYSALLDSMNVLDNVAFPLREHTKLKDKEIRQRVIDKLKALGLENIEKRA
                     PSELSGGMKKRVGLARALMMDPEVVLYDEPTSGLDPISSRVVDDMILETRDRFGVTSV
                     VISHDLGGALRIADEIHVLDKGRVVASGTPHEIVESRHSLVSRFLEASGVAAERLLEE
                     RTQREAKSA"
     gene            complement(2283..2912)
                     /locus_tag="VFQ35_24305"
     CDS             complement(2283..2912)
                     /locus_tag="VFQ35_24305"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET9933858.1"
                     /translation="MSCFDGLRLVAITDLEELPGAKVVERWERLAGLAVPGRVAIDLR
                     GPRAAARSLLELGAALARVARSQQQLLIVNDRLDVARLLAADALHLREDSVTSGEARQ
                     FCPGVPILRACHRPADVALVDADAVLLSPILAARKGNAALGVEALREARARLDAQAGS
                     VRLFALGGIDAAGAPNCIDAGAHGVAAIGAALGAESPRDLLQALRIARS"
     gene            complement(2909..3271)
                     /locus_tag="VFQ35_24310"
     CDS             complement(2909..3271)
                     /locus_tag="VFQ35_24310"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9933859.1"
                     /translation="MILPLAYAAAIFYGGVINIGELPHTRFVASDKLLHAGAFMGLEV
                     LVEWALLARAAQLRHVLAVGAAIGVGALLELVQAALPYRSADIWDLFADSVGACLGAI
                     AWAVFTKIQGSRNQAEPS"
     gene            complement(3307..4599)
                     /locus_tag="VFQ35_24315"
     CDS             complement(3307..4599)
                     /locus_tag="VFQ35_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9933860.1"
                     /translation="MAQPEVEGRDAEPKSLDAAKAAKVPFTPYQRKLFGFLSVACFFE
                     GYDFFALSQLLPNLRAYFGLTEAEGGYLVGTVGVGTVLAYSMVGLADRWGRKRVLTLT
                     IAGYTLFSLFSGLAPSALAFAGFQLLARVFLIGEWATSMVIAAEEYPAERRGLVIGVV
                     SAAAALGSIVCAVLVPLLLKTPLGWRSVYLVGVVPLVLVAYARRSLKETARFESVARS
                     AGGETRVRIADVFRAGYGVRVLQMGAIWFLCYICTQNAVTFWKEFAVHERALTDAAVG
                     KIVAISAVVSMPIAFGAGAFLDAVGRRVGGTVILAVLSVGVLLSYTAESQLLLTLGLM
                     AATIGLNTSLTLLNTLTTELFPTKLRGAAFAWSNNLIGRIGYVLSPLAIGQLAQRLGW
                     GSVLRTTAIFPCVACLLIWIFLPETRGRELEATAEPSA"
     gene            complement(4612..>4821)
                     /locus_tag="VFQ35_24320"
     CDS             complement(4612..>4821)
                     /locus_tag="VFQ35_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET9933861.1"
                     /translation="GTASDAAVAMELGCDGVLMNTAIAGARKPVLMADAMRKAVEAGR
                     QAFRAGRMPKRLYANASSPEAGLIE"
BASE COUNT          897 a         1628 c         1545 g          751 t
ORIGIN      
        1 ttcgtagaag acgaacgact tggagcgctt cgctccctcc gcgagtgacg cctcgaagat
       61 ccccttttgc cgccaaaagt cgaggatctg ctgctcgtac ttcggaaagt cgagctcggt
      121 cggaacggcg gcgaaaggcg tcttatcggt catggggacg gcgggatata ccacaccgtc
      181 ccgcccgacg cgccacgccc ttcgccagcc ttttactgcg gaaagctcag cggtagtagg
      241 ccgcgcagag ctcgatgccg cggtcgaggt cggtcgtcgc gcgccagccg aacgcctcga
      301 aaatgcgggt cgtatcggcg atggagtgca tcacgtcgcc ggcgcgcttg tccttgtgct
      361 cggcgctgcc gttgaaaccg gtcgccttgg caatggcgcg cgcgaggtcc gtgacggtgg
      421 tgcgccggcc cgctccgacg ttgaacgccg agcccgaatg cccgctgccg agcagtgcgg
      481 ccaggttcgc ggcgaccacg tcgcccacgt aagtgaaatc tcggctctgc tgcccgtcac
      541 cgtaaatcac gggggcctgt ccggcgcggc acgccgcgaa gaagcgcggg atcaccgccg
      601 cgtacgcgcc atgtggatct tggcgcgggc cgtacacgtt gaagtaacgg aggccgatga
      661 tttccatccc gtagcagcgc gcgtaaatgt cggtgagctc ttcgtccatg cgcttggaca
      721 gcgcgtacgg cgacaggggc agcccttctt ccccttcacg tttcggcagc ttttcgctcg
      781 tgccgtaaac actcgaggac gaggcgtaga cgatgcgctt cacgcgctga tcgcgcgcag
      841 ccgtgaagac gttgcacgtg ccgctgacat tcacggcgat ggagtcggaa ggcttctcga
      901 tcgagcgcgg caccgagccg agcgcggcct cgtgcaaaat gaagtcgacc gcttcgcacg
      961 cgcgctgaca cgcggcgagg tcccggatgt cgtcctggaa aaactcgatg cgctctttta
     1021 cgtgcgccaa gttttcgagc ttgccggttg cgaggttgtc gagcacccgc accttggctc
     1081 cgcgctcgag caaggcatcg acgagatgcg agccgatgaa tccagcgccg cccgtcacga
     1141 gcacgcgcgc tcccttcaac cgttcaaagt cagtcatttg agcctcagtc cgcttcctgt
     1201 tcgtccagtg agaggtccct cgcgaaagag ggcgccttgc agccgcagtg gaaacggcca
     1261 tttcagtcga gcttcggtcg agcgtcaatc accagacgcg caacacccac cgaatttatc
     1321 ggcgatcgaa cggatgacta cgccgccccc cggcatcgtt ctgcgaggca accttgctcg
     1381 ggcttgcctc ggcgtctgcg cgtgtgagca tgttcggtcg gcatggccgc agagccgcag
     1441 caagatcctt cgcctccgca gaacggcgcg tccatgaagg cgcctccggg cgcggctccg
     1501 cacatcctcg tggaacacct cgagcggcgc ttcgacgact tcgttgcgtt gcacgacatc
     1561 aacatgagca tcggccgcgg gcacgtggcc gtcatcatcg gaggctctgg cgccggcaag
     1621 acgacgctcc tcaagatcct catcgggctc gatcgaccaa ccggtggctc ggtgctgctc
     1681 gaaggcgtcg acatgaccaa gctgaacgac cgcgagctca accggctccg ccgaaagctc
     1741 gggatggtgt tccaatactc ggccctgctc gactcgatga acgtgctcga caacgtcgcc
     1801 ttcccgttgc gtgagcacac gaagctcaag gacaaggaga tccgccagcg cgtgatcgac
     1861 aagctgaagg cgctcggcct cgaaaatatc gagaagcgcg ccccgagcga gctctcgggc
     1921 ggcatgaaga agcgcgtcgg cctcgcgcgg gcgctgatga tggaccccga ggtcgtgctg
     1981 tacgacgaac cgacgagcgg cctcgacccc atcagtagtc gcgtcgtgga cgacatgatt
     2041 ctcgagacgc gcgatcgctt cggcgtgacg agcgtcgtca tctcccacga tctcgggggc
     2101 gcgctgcgca ttgccgacga gattcacgtg ctcgacaaag ggcgcgtcgt ggcctcgggt
     2161 acaccccacg agatcgtcga gtcgcggcac tcgctcgtct cgcgcttcct cgaagcgagc
     2221 ggcgtcgcag ccgaacgcct gctcgaagag cggactcagc gcgaagccaa atccgcctga
     2281 gctcagctcc gcgcgatgcg gagcgcttgc aacaaatcgc gcggcgactc cgccccgagc
     2341 gcggctccaa tcgcggcgac gccatgagcg cccgcatcga tgcagttagg ggctccggcc
     2401 gcatcgattc cgccgagcgc gaacagccgc actgagcctg cctgcgcgtc gagccgcgcg
     2461 cgcgcttcgc gcaaagcttc gaccccgagc gcggcattgc cctttcgcgc tgccagaatg
     2521 ggcgacagga gcaccgcgtc cgcgtcgacc agcgcgacgt cagctggacg gtggcaagcc
     2581 cgcaggatcg gcacgccggg acagaactga cgcgcctcgc cgctcgtcac cgagtcttcg
     2641 cgcaggtgaa gcgcgtctgc ggcgaggaga cgcgcgacgt ccaagcggtc gttcacgatg
     2701 agaagctgct gctgactgcg agcaacgcga gcgagcgccg cgccgagctc caagagcgag
     2761 cgcgctgccg cgcgcggacc gcgtagatcg atcgcgacgc ggcccggcac tgcgagccca
     2821 gcgaggcgct cccagcgctc gacgaccttc gcgccgggga gctcttcgag atccgtgatc
     2881 gcgacgagcc gaagcccgtc gaagcagctc acgacggctc cgcttggttc cggctcccct
     2941 gaatcttggt gaaaacggcc catgcgatcg cacccaagca ggcgccgacg ctatccgcga
     3001 acaagtccca gatgtccgcc gaccggtacg ggagggccgc ttgaacgagc tcgagcaagg
     3061 cgccgacacc gatggcagcg cccacggcga gaacgtggcg gagctgcgcg gcgcgggcga
     3121 gcagcgccca ctcgacgagc acttcgagcc ccatgaacgc gcccgcgtgc aacagcttgt
     3181 cgctcgcgac gaagcgcgtg tgcggcagct cgccgatgtt gatgacgcct ccgtagaaga
     3241 tcgcggccgc gtaggccaga ggcaaaatca cacgaagtct gtcgagagta ctctgaggtc
     3301 gactgctcac gcgctcggct ccgccgtcgc ctcgagctca cgcccgcgcg tctccggcaa
     3361 aaagatccag atgagaaggc aggctacaca cgggaaaatc gcggtcgtgc gcagcaccga
     3421 accccagccg aggcgctgcg cgagttgacc aatcgcgagc ggcgaaagca cgtagccgat
     3481 acggccgatc aaattgttgg accaggcgaa cgcagcgcca cggagcttgg tcgggaagag
     3541 ctccgtcgtc agtgtattga gcaacgtgag gctcgtgttg aggccgatgg tcgctgccat
     3601 caggccgagc gtgaggagca gctggctctc ggccgtgtaa ctcagcagca cccccaccga
     3661 cagcacggcg aggatgacgg tgccccccac gcggcggccg acggcgtcga ggaaagcgcc
     3721 cgcgccgaac gcgatcggca tggacacgac cgccgaaatt gcgacgattt tgccgacggc
     3781 cgcgtcggtc agagctcgct cgtgcaccgc gaactctttc cagaacgtca ccgcgttctg
     3841 cgtgcagata tagcagagaa accagatcgc ccccatttgg aggacgcgca ccccgtagcc
     3901 agcgcgaaag acgtcggcga tgcggacacg tgtttctccg cccgcgctcc gtgcgacgct
     3961 ttcgaagcgc gcggtctctt tgagagagcg gcgcgcgtac gccacgagca cgagcgggac
     4021 gacaccgacc aggtacaccg agcgccagcc gagcggcgtc ttcaagagga gagggacgag
     4081 caccgcgcac acgatgctgc ccaacgcggc cgccgcgctc accacaccga tgaccaagcc
     4141 tcggcgctcg gccggatact cttcggccgc gatcaccatg ctcgttgccc attcgccaat
     4201 caagaacacc cgcgccagaa gctggaagcc agcaaacgcc agtgcgctcg gcgctagtcc
     4261 cgaaaagagc gaaaagagcg tgtaccccgc gatcgtcagc gtgaggactc gttttcgtcc
     4321 ccagcgatcg gcgaggccga ccatcgaata ggcgagcacg gtccccacgc cgaccgtgcc
     4381 caccaggtaa cccccttcgg cttcggtgag accgaagtaa gcccggagat tcggaagcag
     4441 ttggctcagg gcgaagaagt cgtacccctc gaaaaagcac gcgacgctca gaaagccgaa
     4501 cagcttgcgc tggtacggcg tgaaaggaac cttcgcggct tttgcggcgt cgagcgattt
     4561 cggctcggcg tcgcgcccct cgacttcagg ttgcgccatt tggtctggcg ctcactcgat
     4621 gagcccggct tcgggactcg aagcgtttgc gtacaagcgc tttggcatgc ggccagcacg
     4681 aaacgcctgg cgaccggctt cgaccgcctt gcgcatcgcg tccgccatca acaccggctt
     4741 gcgggcgccc gcgattgcgg tgttcatgag cactccgtcg cagccgagct ccatcgcgac
     4801 ggccgcgtcg ctcgcggtgc c
//
LOCUS       DASTKS010001146         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_404418, whole genome shotgun sequence.
ACCESSION   DASTKS010001146 DASTKS010000000
VERSION     DASTKS010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404418"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ35_24325"
     CDS             complement(<1..258)
                     /locus_tag="VFQ35_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933862.1"
                     /translation="MASESLRQPVVSTLAHRPAAPLDAHERRATSETVGRLSASGPSS
                     AWLAALPWVAIALVVLAWLPALDAPYQYDDYNTPVGDAASQS"
     gene            complement(494..853)
                     /locus_tag="VFQ35_24330"
     CDS             complement(494..853)
                     /locus_tag="VFQ35_24330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933863.1"
                     /translation="MPQSPKFARLIASLLAAGVLFACSGDGLGDSSGSVCPTDSALTY
                     ENFGQNFMNTYCVACHASKESPHLGTVEQVRAHIGEIDRAAASGPKATNTYMPEGGSV
                     PTEERQKLGEWLACGAP"
     gene            complement(1113..1670)
                     /locus_tag="VFQ35_24335"
     CDS             complement(1113..1670)
                     /locus_tag="VFQ35_24335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933864.1"
                     /translation="MPQVKGVSFKSVMSAVEALHGDAVAAGCLAELAGDVGSALRYRG
                     IVATGWYPIEWYRALLHSVRNVVGQGEPGLYAIGRQCAKQDMTGIYRLGFKLLAPETV
                     LGLSTRLFSNYYDTGKSRVIESRPGFAHVRWWDCAGFDRNMWCEAFGAAEMFMQLAGA
                     ATIRSRPIWRSDNAHDAVELTMHWV"
     gene            1785..2324
                     /locus_tag="VFQ35_24340"
     CDS             1785..2324
                     /locus_tag="VFQ35_24340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933865.1"
                     /translation="MSQLASFEFATCERDSASCYGVFENILIIFGHGPPDATNAKLFS
                     ALVTDLAQRYPRGAGVLVVVQTGSSPTPDGREALLAAFRALHGAVSFSFVLESDSFAA
                     VAQRAIIKTFLFVTRYRDCIRIEPNVEAAARWLCARVKRANGKTWDVPAVQQAARAFC
                     SQQDGYYRGDESEEGRVGV"
     gene            complement(2529..2948)
                     /locus_tag="VFQ35_24345"
     CDS             complement(2529..2948)
                     /locus_tag="VFQ35_24345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933866.1"
                     /translation="MGAGSTCSIGACRGGCRLLCSDGATCTLASCTGGCSIGCDGALC
                     QVDECGAGCSIECAPGSTCEIGTCTEGSCHIGPSGSGTEPSTLLIHECAGGHCDIECG
                     PGNACSIESCTGGDCTITCRAGATCKCAASGCAIDQL"
     gene            4314..4535
                     /locus_tag="VFQ35_24350"
     CDS             4314..4535
                     /locus_tag="VFQ35_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933867.1"
                     /translation="MSRSAHLLHAALAREEGTSIVVYFRIHSLTHRLAPRTFDVRARR
                     RARNVSVAHERVDSSSLEGESVVVRESKS"
     gene            4642..>5526
                     /locus_tag="VFQ35_24355"
     CDS             4642..>5526
                     /locus_tag="VFQ35_24355"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9933868.1"
                     /translation="MANRNEERTRARRLGLGLNATSVLFLTPLTLLGCSTSTEPERSV
                     GVTAQAVEAANCGYSLCVEVKKASKKGFSATIKITNRGGRLATSGFTVLVNSGAAQLT
                     HVGHGTFKAVEGGYLLSTVGSAESADDLDGDAADDPDVLSGRAYRFHLKFSGVYTTLK
                     ANIISSGGVNCDQTAPIVKLATNSDFFTANGSLVLTADATDNSAVAKVEFSRDGVVIG
                     TAKSPPYVLSVPVTNASNGRHRYAATAYDLTGNAASVTKRVLVAIGNKFFGTAATTAA
                     DYPNLLAHFDQITPGNAGK"
BASE COUNT          971 a         1791 c         1769 g          995 t
ORIGIN      
        1 cgattgagac gcagcgtctc cgacgggcgt gttgtagtcg tcgtactgat agggtgcgtc
       61 gagcgccggc agccaagcga gcacgacgag ggcgatcgcg acccacggca gagcggcgag
      121 ccaggccgac gacgggccgg aagccgaaag ccggccgacg gtctcgctgg tcgcgcgcct
      181 ttcgtgcgcg tcgagcggcg ccgcggggcg gtgggcgagc gtcgacacga cgggctggcg
      241 caaggactct gaagccatca aagacggaac ctcagagcat cttcgacgcg cgacgcgact
      301 tcaacagcga ttttggtgga gccgttcgtt cgctagcgga cgctcggccc gcagcggcgt
      361 ccggggctca ctcctcgccc gaccgcctcg cttcggtccc gactagctcg cgactggctc
      421 atccactcgc ggcccttggg tctcgccgcg ttggaacaac accgagccat ggacgcgctt
      481 gccgagggga accctacggc gcaccacaag cgagccactc gccgagtttt tgacgctcct
      541 cggtcggcac cgagccgccc tcaggcatgt acgtgttggt tgccttgggg ccggacgcag
      601 ccgctcggtc gatttcgccg atgtgtgcgc ggacttgctc caccgtgccc agatgcggag
      661 actccttgct cgcgtggcag gcgacgcagt aggtgttcat gaagttttgc ccgaagtttt
      721 cgtacgtgag cgcggagtca gtggggcaaa cgcttccgct cgaatcgccg aggccgtcgc
      781 cgctgcaggc gaacagcacg ccggcggcaa gaagggaggc gatgagccga gcaaacttgg
      841 gtgattgggg catcgttctc ggattgttcc tcttcgcgac gagaacatgc atgcattcga
      901 caggtaagtt cggctgcgcc tagcaggagt cggtcggtgt gcgagccgcg cgcggcgcgc
      961 gatgaaacga accagcggcg cacgttttgc gttgcacgat ttttgcggcg ttttccgcgt
     1021 gccgccggga tgctttttgt agcggagagg cctctcccga aagccagatg aatggtgcgg
     1081 ccccgcgaga ctcgcgcgcc gcgccctctg gatcacaccc aatgcatcgt gagttcgacc
     1141 gcgtcgtgcg cgttgtcgct gcgccaaatt gggcgcgaac ggatggtggc cgcgcccgcg
     1201 agctgcatga acatctcggc cgcgccgaac gcttcgcacc acatgttccg atcgaagccc
     1261 gcgcaatccc accaacgcac gtgcgcgaag ccgggccgcg actcgatgac gcgacttttc
     1321 ccagtgtcgt aatagttcga aaagagccgt gtcgagagcc ccagcacggt ctctggggcg
     1381 agcaacttga acccgagccg gtagatgccg gtcatgtctt gcttcgcgca ttggcggccg
     1441 atcgcgtaaa ggcctggctc accctgtccg acgacgtttc gcacgctgtg cagcagggct
     1501 cgataccatt cgatcgggta ccagcccgtc gcgacgattc ctcgatagcg cagggccgag
     1561 ccgacatcac ccgcgagttc cgccaggcac cccgcggcca cggcatcacc gtggagcgct
     1621 tctaccgcgc tcatcacgct cttgaaagat acccccttca cttgcggcat ggcacttcgg
     1681 ttttgagcct tccgtcgtgg tctgtccagg tgtgtgaacg ggtgtctcgg ggtacgtctt
     1741 aagtcgcccc agtcgggtac cgttgtcatc ggcagagggt gaccgtgtcg cagctagctt
     1801 cattcgagtt cgcaacctgt gaacgagact cggcgtcgtg ctacggggtg ttcgaaaaca
     1861 tactgatcat cttcggacat ggaccgcccg atgcgacgaa cgcgaagttg ttctcggcgc
     1921 tcgtcaccga cttggcccag cgctatccgc gcggagcggg tgtgctcgtc gtcgtacaga
     1981 ccggctcgtc cccgacgccc gatggtcgag aagcgttgct cgctgcgttc cgggcgttgc
     2041 acggcgcggt ttcattctcg ttcgttctcg agtcggatag cttcgccgcg gtcgcgcagc
     2101 gcgcgatcat caagacgttt cttttcgtca cccgctatcg tgattgcatt cgcatcgagc
     2161 cgaacgtcga agcggcagcg cgctggctgt gtgcacgcgt caaacgcgca aatggtaaga
     2221 catgggacgt gccggcggtg cagcaagcag cgcgtgcttt ttgctcgcag caagacgggt
     2281 actatcgcgg cgacgaatca gaggaagggc gcgttggagt ctagtcatcg gcgtcgaaac
     2341 cgtcgcttcg tcggcgtcta ctgatcgacg tcgaagcctt ggctcgtccc gcgaggcaac
     2401 gcactggccg ccgcgcactt tctctcgtcg agcgcaacca tcgaaggcct cggctgcgct
     2461 cttgccatca gcgcgcttgc actcgagttc ggtcgtgtat cgcgtcagct ctggccacag
     2521 cgttgggttc agagctgatc gatcgcacat ccgctcgcag cgcacttgca ggtcgctccg
     2581 gcacggcacg tgatggtgca gtcgccgccc gtacagcttt cgattgagca ggcgttgccg
     2641 ggcccgcatt cgatgtcgca gtgaccgccc gcgcactcgt ggatgagcag ggtggacggc
     2701 tcagttccgc taccgctcgg gccaatgtgg cagctgccct cggtacacgt gccgatttcg
     2761 caggtactgc cgggcgcaca ctcgatggag cagcctgcgc cgcattcgtc cacttggcag
     2821 agcgcgccgt cgcaaccgat gctgcagccg cccgtacagc tcgcgagcgt acaggtagcg
     2881 ccgtcgctgc agagtagtcg acagcctccc cgacaggcgc caatgctgca ggtggagccg
     2941 gcacccacgc actcgctcct gcagccgccg gtggggcact ccgttttaca ttggccctcc
     3001 gcatcgcagc ggctactgca gccgccctcg gcgcagtcga tgtcacattg cgcggcgtga
     3061 catccgagat cgcagccgcc caagcatgcg agcttgcacg tcgacgtgtc gcacgagccc
     3121 gcacaggcgc cttttggaca cgcaagtgca cagctcgact tactgcggca gtcgaagttg
     3181 cacgccgtcg tgtcagatgc gtcgcaggtg agcgagcacg tctcaccatt gcacgagcac
     3241 ggaccgtcgg tgcagggttg cgagcgcagt cctcctgctc ctccgtcgcc agtgctcgac
     3301 gtcccgccgc tctgcgcgcc tgcggacgcg gtgcctcccg tcgctgtgaa aggtgaggag
     3361 ccacctgcac cagctgaagc gcctccgctc gtagccgcgc cgccttttgc ggcggagcca
     3421 cccttgccgg ctccggcgga tgcgaagccg ccgaaagcgc ctccctgcgc ggagccaaag
     3481 ccgcctccct gcggcgagcc tgatgcgctc cccgagaagc ccaaagcgcc tccgtggccc
     3541 tgtgagcgcg cgctctctcc gccgcacgcc agcgcgaacg caagcgagac gagcgatccg
     3601 cgaacgagtt cgatgtgtct ccgcattttg ccgatcccct gagactcacc atactcgcaa
     3661 caaagcgtgg cagggcatcg cgttgaccgt agatcttgcc cgttattgct gaaagagagt
     3721 cgtttgcata ctggagccgg acatttccga cggggcggca gcggtttccc gcgcccacgg
     3781 cattggtcag ccgcctcagc agggcgtttt tgaacgccgg ctaggcgttg gccgcgactc
     3841 gcgtgcgaag cgagcgaggc gccgttcgac gcagggccga cgaacagcat gctgcgcgca
     3901 tgacgattgt cttcagtccg aggatgctcc gagccaagag tgctccgagc caagagtgct
     3961 ccaagccaaa cgctccaagc caaacgctcc aagccaaaac gctccaagcc aaacgctcag
     4021 agacctcggg ttggtctcca gcggtcgctt tgcggtcgac gcatcgagct gagtcagcgc
     4081 gagatctgag tcggcgtacg agttctctac gacgatgagc gtttcggtgg gcgcttggta
     4141 cgccgcgcga acgctctgcg tgcgtggagc atgctggacg caagcgatgc agggcggtca
     4201 gtcgaacatc gcgcgtgctg actcgctcgc ggcgaaactt accaggcaaa aatcaccaag
     4261 ttttggcgca aaagcgccgc gcattcgtgc tgcggctcga ccctgtcgga atcgtgagca
     4321 ggtcggcaca ccttctgcac gccgcgctcg ccagggaaga aggcacctca atcgtagtct
     4381 actttcgaat tcactcactc acgcaccgac tcgctccgcg cacgttcgac gtgcgggcgc
     4441 gtcgacgggc gcgcaacgtg agcgtcgcac acgagcgagt cgattcgagc tcgctcgagg
     4501 gcgagtccgt cgtcgtccgc gaatcgaaga gctgacccaa gtcatcggcg gtgcgttcga
     4561 gcactagctc gagcatgccc gatgacggtc ggagcgactc ccttttcacg agggatgttc
     4621 cgggaaatga agagatgcaa aatggcgaat cgaaatgaag agcgcacccg agcgcgcagg
     4681 ttgggcctcg gcctgaatgc gacttcggtc ctgtttctta cacctctgac gttgctcggg
     4741 tgctcgactt ccaccgagcc ggagcggtcg gtgggcgtca cggctcaagc ggtagaagcc
     4801 gccaactgcg gctactcgct gtgcgtcgaa gtgaagaagg cgagcaagaa ggggttctcg
     4861 gcgaccatca agatcacgaa cagaggtggg cgcctcgcga cgagcggctt tacggtgctc
     4921 gtgaattcgg gcgcggctca gctgacccac gttgggcacg gcaccttcaa ggccgtcgag
     4981 ggtggttacc tgttgagcac ggttggttcg gccgagagcg ccgacgacct ggacggcgac
     5041 gccgcggacg atcccgacgt tctgagtggc cgcgcctacc gatttcacct gaagttctcg
     5101 ggcgtgtaca cgaccctcaa ggcgaacatc atctcgagcg gtggggtaaa ctgcgatcag
     5161 acggcaccga tcgtcaagct cgcgacgaac agcgacttct tcaccgccaa cggctcgctc
     5221 gtgttgaccg ccgacgcgac tgacaactcg gccgtcgcca aagtagagtt ctcgcgggac
     5281 ggcgtggtga ttggcacggc caagagcccg ccctacgtgc tcagcgttcc ggtgactaac
     5341 gcgagcaacg ggcggcaccg gtacgctgcc accgcgtacg acttgactgg caacgcggcg
     5401 agcgtgacca aacgcgtgct cgtcgcgatc gggaacaagt tcttcggtac cgcggcgacc
     5461 accgccgccg actatcccaa cttgctcgcg cacttcgacc agattacgcc gggaaacgcc
     5521 ggcaag
//
LOCUS       DASTKS010001147         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_404515, whole genome shotgun sequence.
ACCESSION   DASTKS010001147 DASTKS010000000
VERSION     DASTKS010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404515"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFQ35_24360"
     CDS             <1..1094
                     /locus_tag="VFQ35_24360"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9933869.1"
                     /translation="SAAGQGVGDAMEAVVLGRVRLSMRVTLAKSFASFCAIASGGSLG
                     REGPIIQLGAASGKLVGTFAGLSSRRRRTLIAAGTAAGFAAAYNTPFAAVLFVLEVVI
                     GVVALDVIVPCLGATAIATALTRALVGPGPVYGQREFSWVGTKELLAFAVLGLLGAVF
                     ARLFMLVVRGAETAFRRLALPPPLRGALGGFGAGLGVLLVPEVAGNGYEPLNALLDGT
                     PLVSFVLVLVVVKSFATASSVASGSPGGVFTPTMLIGGACGFLFHAFLHRFTSFAPGP
                     AGGFALVGMAAATAATTHAPLMAAVMAFELSGDYAVVLPLLVATALATALSRSLSKDS
                     IYIAELRRRGVAWELTLEGRQMSSDTKES"
     gene            complement(1107..2495)
                     /locus_tag="VFQ35_24365"
     CDS             complement(1107..2495)
                     /locus_tag="VFQ35_24365"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9933870.1"
                     /translation="MDRSVVEVASELAARAHNDQVRKSGGLPYFTHLEAVARLVEEHG
                     HDDPVVIAAAYLHDLLEDRPAFADELRARMPLEVVETVEALSEVKHDEQGRRLPKSVR
                     FERYLRGLSQRSRITERALPISCADKIHNVESLIEAESRGEPLFLKLNTRPGEQLAHL
                     ARLRPLYADVVAPSLLARFDHAHRALTATVERWLFGRAVMLAAEAHLSQYDRAGQPYI
                     LHPLTLSLNAETTDERIVALLHDVVEDSPLTLDALTREGFSERVVRAVDALTKRPGES
                     YDVFIERVAKDRLATRVKLLDLAHNSDLSRLPNPTDADRARTEKYARASEYLRAELAK
                     RSLQIRLSDPSVERVRAAKRLPVARGEHVTLARRVAPDTSLLALVPANHQFGEAIPMR
                     AVAECCNERVQAWVIEVSGSRVRPWDGGVLHVTVSRAEDARSKDSNELLLSAPETPIS
                     VELDGQLEWVDS"
     gene            2713..>3861
                     /locus_tag="VFQ35_24370"
     CDS             2713..>3861
                     /locus_tag="VFQ35_24370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933871.1"
                     /translation="MPNTRILALLTLSVVAAAVGCGDGGGGSSAAGGRPSASGGASSE
                     GGTSAVGGTSLGGASTGGGTSAGGGAISSGGSGGDATGGRATGGVAGSAGNTGGRPQG
                     GAPNAGGGAKSSGGAANGGANSNSGGQASGGATAAGGSSGGGPVGGASAYPCDGTTTG
                     YDATVVLNGSTWTVTRNGTTVSTSTDMATAMYAGINSLPANRSVKQSVLVQGSGAIPA
                     NSQTNLPNFLVLNVCGTIEVTGAPSGSDKAPIYARGRHDIEIPNLKMTGAPMYGMFFR
                     NVDNLHLGDIELRLSGGLGVRIDNNAQTQKNTNVVIDRVYGEGTGSHVVETYGIDKIK
                     IGTITGKDVGECGLLLNNTTNAEVDLVTCDNCGAGTGYAAFRIANDAGK"
BASE COUNT          606 a         1249 c         1348 g          658 t
ORIGIN      
        1 agagcgccgc gggccaaggc gtcggtgacg cgatggaagc ggtggtgctc gggcgcgtgc
       61 gcctctcgat gcgggtcacg ctcgcgaagt ctttcgcatc gttttgcgcg atcgcgagcg
      121 gtggctcact cgggcgcgaa gggccgatca tccagctcgg ggctgccagc ggtaagctcg
      181 tcggcacctt cgcggggttg tcttcgcggc gccggcgcac gttgatcgcg gcggggacgg
      241 cggccggatt cgccgcggcg tacaatacgc cgttcgcggc ggtgctcttc gtgctcgagg
      301 tggtcatcgg cgtcgtggcg ctcgacgtca tcgttccgtg cctcggcgca accgcgatcg
      361 cgaccgcgct cactcgagct ttggtcggac cggggccagt gtacggccaa cgtgagttca
      421 gctgggtcgg tacgaaggag ctgctcgcgt tcgccgtgct cggcctgctc ggcgccgttt
      481 ttgcgcggct cttcatgctc gtcgtgcgcg gcgccgagac tgcgttccgg cgtctcgcgt
      541 taccgccacc gttgcggggc gccctcggtg gcttcggcgc tgggctcggt gtgctcctcg
      601 tgccagaggt tgcgggcaac gggtacgagc cgctgaacgc gctactcgat gggacgccgc
      661 tcgtttcgtt cgtgctcgtg ctcgtcgtcg tcaaaagctt tgcgacggcg tcgtcggtgg
      721 cgtcgggcag cccgggtggc gtgttcacgc cgacgatgct catcgggggc gcgtgcgggt
      781 tcctgtttca cgccttcctc catcgcttca cgtcgttcgc tcccggtccc gccggtggat
      841 tcgcgctcgt cgggatggct gcggcgacgg cggcgacgac tcacgctccg ctgatggcgg
      901 cggtgatggc gttcgagctc tccggcgact atgcggtcgt gttgcctctc ttggtcgcga
      961 ccgctctggc gacggctctg tcgcgctccc tttcgaaaga ctcgatttac atcgcagagc
     1021 tccggcgccg cggcgtcgcg tgggagctca cgctcgaagg tcgccagatg agcagcgaca
     1081 cgaaggaatc gtgagcgcgc tcgtcctcac gaatccaccc actcgagctg cccatcgagc
     1141 tccaccgaga tcggcgtttc gggcgcggag agcaagagtt cgttcgagtc cttcgagcga
     1201 gcgtcttcgg ctcggctcac ggtgacgtgc agcacgccgc cgtcccaggg gcgcacgcgg
     1261 ctgccgctca cctcgatgac ccaggcctga acacgctcgt tgcagcactc ggcgacggcg
     1321 cgcatcggaa tggcttcgcc gaattgatgg ttggcgggca cgagcgccag gagagacgta
     1381 tccggcgcga cgcgacgagc caaggtgacg tgctcgcccc gcgcgacggg cagccgcttc
     1441 gccgcgcgca cgcgttcgac gctcgggtcc gaaaggcgga tctgcaagct tctcttcgcg
     1501 agctctgcgc gcaaatactc gcttgcgcgc gcgtacttct cggtgcgcgc gcgatcggcg
     1561 tcggtcgggt tcggcaagcg cgacaagtcc gaattgtgcg cgaggtcgag cagctttacc
     1621 cgcgtcgcga gacggtcctt cgcgacgcgc tcgatgaaca cgtcgtagct ctcaccgggt
     1681 cgcttcgtga gcgcgtccac ggcgcgcacg acgcgctccg aaaaaccttc gcgcgtcaac
     1741 gcatcgagcg tcagcgggct gtcttcgacc acgtcgtgca gcagcgccac gatgcgctcg
     1801 tcggtggtct cggcgttcag gctgagcgtg agcgggtgta aaatgtatgg ttgccccgcg
     1861 cgatcgtact ggctgaggtg cgcctcggcc gcgagcatca cggcgcgccc gaacagccag
     1921 cgctcgacgg tcgccgtgag cgctcggtgg gcgtggtcga atcgcgcgag tagggaaggc
     1981 gcgacgacat cggcgtagag cggccgcagg cgcgcgagat gcgcgagctg ctctcccggg
     2041 cgagtgttga gcttcaagaa cagcggctct ccgcggctct cggcttcgat caggctctcg
     2101 acgttgtgga tcttgtcggc gcagcttatc ggcagcgcgc gctcggtgat tcgtgagcgc
     2161 tggctcagcc ctcgcaggta gcgctcgaag cgcaccgact tcggtagccg ccgtccctgc
     2221 tcgtcgtgct tgacctcact cagggcctct accgtctcta cgacctcgag gggcatcctg
     2281 gcgcgcagct cgtccgcgaa cgcgggccga tcctccaata gatcgtgcag atatgcggcc
     2341 gcgatgacca cggggtcgtc gtgcccatgc tcctcgacga gcctcgcgac ggcctcgagg
     2401 tgcgtaaagt acggcagccc gccagattta cggacttggt cgttgtgagc tcgcgccgca
     2461 agttcactcg ccacctcgac gacgctccgg tccacccggc aaagatatca gaaggagtaa
     2521 agtgggatcg aagtgatcgc agtcgagcgg aattcccgag gaaatgcgct cctgcttgac
     2581 gagttggcgg cgcgcgtacc ctaactgttt gcagagcgca tgcggcccat catgcaatag
     2641 gtgcggcaag cagcgatgag cccgcgccgc gacgccccga gcgttctact ggacacggac
     2701 acggaccaaa caatgcccaa tactcgaatc cttgcgctgc tgacgctctc cgtggtcgcg
     2761 gctgcggttg gctgcggaga tgggggcggc ggctcgtctg ccgcgggcgg aagaccttcg
     2821 gccagcggtg gcgcgagctc tgaagggggc acgagcgcgg ttggcggaac ctccttgggc
     2881 ggagcttcga cggggggtgg cacgtcagca ggcggcggcg ctatctcgag cggcggctcg
     2941 ggaggcgacg ctaccggagg ccgcgcgacg ggcggcgtag ccgggagcgc agggaacacg
     3001 ggcgggcgtc cacagggtgg cgctccgaac gccgggggcg gcgcgaagtc gagtggaggc
     3061 gcagcgaacg gcggagcgaa ctcgaacagc ggcgggcaag cgagcggcgg tgcgacggca
     3121 gccggcggtt cctcgggggg tggccccgtc ggcggcgctt ccgcatatcc gtgcgacggc
     3181 acgaccacgg gttacgacgc cacggtcgtt ctgaacggca gcacctggac cgtgacacgc
     3241 aacggcacga cggtatcgac ttcgaccgac atggccacgg caatgtatgc cggcatcaac
     3301 agcctgcccg cgaatcgcag cgtgaagcaa agcgtactgg tgcaaggctc cggcgccatt
     3361 cccgccaact cgcagacgaa tttgcccaac tttctcgtgc tcaacgtgtg cgggacgatc
     3421 gaggtgacgg gggctccgag cggctctgac aaagcgccga tctacgcgcg cggccgccat
     3481 gacatcgaga ttccgaatct gaagatgacc ggcgcgccca tgtacggcat gttctttcgc
     3541 aacgtcgaca acctgcacct cggcgacatc gagctcaggc tgagcggcgg cctcggtgtg
     3601 cgcatcgaca acaacgcgca gacacagaag aacaccaacg tcgtcatcga ccgcgtctac
     3661 ggcgaaggta ccgggagcca cgtcgtcgaa acgtacggca tcgacaagat caagatcggc
     3721 accatcaccg gcaaggacgt cggcgagtgc gggctgctct tgaacaacac gacgaacgcc
     3781 gaggtcgatc tcgtgacgtg cgacaactgc ggcgccggca cgggctacgc ggcgtttcga
     3841 atcgccaacg acgccggcaa g
//
LOCUS       DASTKS010001148        12411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1300255, whole genome shotgun sequence.
ACCESSION   DASTKS010001148 DASTKS010000000
VERSION     DASTKS010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300255"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFQ35_24375"
     CDS             <1..614
                     /locus_tag="VFQ35_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933872.1"
                     /translation="VSTRLVWLLAWLCFVLFQGPFTIAASQLMFSRSVALRSVLAQFG
                     RGVLRYSVGKVMGGMLLAGGAALVLLVPAVVARVLYLGEVTLVEGTGVAASYRRSTRL
                     TKNRVGAALAVALGLIGLAFGFVVCAHVLIEAVAENLLGLGSVSAFWENGPNPWSCMG
                     LLCAAPFVATARFLAYIDCRTRREAWDVQVDFMRLAAAVKRSA"
     gene            611..2566
                     /locus_tag="VFQ35_24380"
     CDS             611..2566
                     /locus_tag="VFQ35_24380"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET9933873.1"
                     /translation="MRLAFARRSCMVSVRRSRLALGFAALAAALSFAVPASAEEGSSE
                     PPRAPLDVDARERLERDLSRLVAGPELAFCHDATEAMRRANREVCRVVLTNARQRCPA
                     MARACSAHFAELESNGSDEARFGQVPGALGSILFWGALVLLGAAVLRFLWPLLESLAP
                     APDVVQKPSAGPSPTEPTTPRPPQESDVERLLAQAERARNEGRFEHAVAALYGALVHF
                     LGREQLITVEPARTNGDYLRELSPQPELAASVRQAFRSVERVQFGHSEATAELCSELW
                     SRILPIVKGGVALLALVLLPHTLIGCGPRASLNPESLPNGHHLLTKLLRERGREVRHL
                     ALSTGEVDDDVSQLLVFGRDTSPEVWSTVLAWAEEGGSVIVTEPSEELTRALHVDREG
                     SFACTHALELAPRALAQSLTVIDAGARRLRLPDAPGSKSAPRVEASCDGSARIATFHY
                     GEGYVTVLANPELFGNISLTVGDNAAFVLELFGPPRGALGLVGSWTGGGVTSPLRALQ
                     NAGLSALMLELLVVLLVFFWHRGAAFGIPRDPSDLARRAFADHVRALGRSYARAKANR
                     HALSQYAGFALEVLRERFGRGAPLSLIDLAGEVARRTGEREGEVMRLLTEAHEAAQDP
                     GPDHGHPADLQTIEKLETLLYPSGGPQ"
     gene            2563..3522
                     /locus_tag="VFQ35_24385"
     CDS             2563..3522
                     /locus_tag="VFQ35_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9933874.1"
                     /translation="MNVEEVSATLKRLSAEIARVLVGQDTLIEHTLIGLLARGHLLIE
                     GVPGLGKTLLVRCLGRVLGLSFRRIQFTPDLMPSDVTGSNVYSEAEGRFSFVPGPVFA
                     QLVLADEINRAPAKTQSALLEAMQDRSVTVDGETRALPAPFLVLATQNPVESQGTYPL
                     PEAQLDRFLLKLEVRHPTRQAEANMLKNYLAGFDPTDLGALTVLSSESHVLEMQRALG
                     EVRVASEILDYITDIVQRTRSHPALTLGASPRASIALLCVARARALWEGRDFVTPDDV
                     KSYAHAVLRHRLLLQPDAELEGTTADHCIDGILRDTVVPGTSA"
     gene            3525..4835
                     /locus_tag="VFQ35_24390"
     CDS             3525..4835
                     /locus_tag="VFQ35_24390"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9933875.1"
                     /translation="MPLVPSRVLVWSSAVPLVLTALALLDERFRGLAMWVNAALVVLA
                     SLDALFARRALVAVERFAPDVMSLKRRNEVRLFVRSSSRRKFVVRVRDDLFEGAAAAD
                     LPLRITLEPRSGRNASYHVEPSQRGAFELGDHNVRYPSPLGFWDRQLRLRARTAVKVY
                     PDLRQIQMFDQLARESRELEWVRSSKRKGGETEFSRLREYVAGDEYRAVDWKATARRQ
                     QLTVREYQLESNQNIVFMLDGGRMMTGIESGLSRFDHALNATLMLGHVAARGGDRVGV
                     LGFDEAVRAFVAPASGPRAVRRLIQATYDLYPRFVDADYDAAFGYLSQRLRARALVVI
                     FSHVFDASVAQTLLRRVRAVRTRHLPLVVMFRDRDVEKLLEPGSAEGADHFTKAAAAE
                     VLRFQKSVMRSMVRAGALLLETDAASLTGKLVSRYLEIKARNQL"
     gene            complement(4844..5293)
                     /locus_tag="VFQ35_24395"
     CDS             complement(4844..5293)
                     /locus_tag="VFQ35_24395"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9933876.1"
                     /translation="MSQLENPYAPPEADDEPAPSSAQEVVVASQGQRFANFVVDSIAS
                     RIVAALPMIIPEMPPAVGGILGVTFFFGYYLVGETAFRATLGKLITKTRVVAANGGSP
                     RFTQILGRTFARFVPFEPFSYLSRSPVGWHDRWSGTRVVTLSSLREN"
     gene            5720..6007
                     /locus_tag="VFQ35_24400"
     CDS             5720..6007
                     /locus_tag="VFQ35_24400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933877.1"
                     /translation="MSCADLLSSPEQWNAAYDACWEQAKARVPVTKSVRTFCEAYTLA
                     EFDCRYWYATEECERDFGMWSERIRNTVAACAMNPSCSEAEECVAAVFRTQ"
     gene            6004..7227
                     /locus_tag="VFQ35_24405"
     CDS             6004..7227
                     /locus_tag="VFQ35_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933878.1"
                     /translation="MKSILFVLVPLAIGCSSEDDGALGAPPLHPVPGCEGIEPTSCDV
                     RLPECQSRLLRLTACLRGEDVMVPPPIRVVSEADFTSELTAQFAEQMQDPNSGHVEAG
                     LNMLGMVELGALTAQARAEDYAKSIAGLYNGETKDIAIIDHGDSLAAERASPLLVHEF
                     VHALQDRDLGLDAFKAQATSNDDTWAKVAVIEGEAQLHEMRYAAAMLGLDSDKVDWKA
                     RFQNALAHDEQLLLKEKSPYLATYKYFPYEWGGRYMYFTWAAGGMSAVNGRFARPPST
                     THTLLASTMMAIDSEITPETLTPPPPSAEWTQVLATTLGAWGVYLAFQKAAPTLDAAR
                     AIALAWRGDSLAVYAPSESGSHTAVVWSLELADEGAAAAVAQIAPALAANVRQAGTRI
                     VIAKTDGSGALDWAF"
     gene            complement(7277..9412)
                     /locus_tag="VFQ35_24410"
     CDS             complement(7277..9412)
                     /locus_tag="VFQ35_24410"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9933879.1"
                     /translation="MRFSGLVVGLTLLALWGCGTSAPTRQGGAGGGGGAGGGGPKGGA
                     LGAGGGSACSSGMTSCDGQCVDLRTNSGHCGTCNNACASGRVCNASTCGCPSGQTLCG
                     NSCVTGANCNAGGSGAGGSGGSTANAGAAGGPLAGNAGAAGAGQAAVDLRCNVGGTQV
                     NVAAADVISDFRQNTPTMYAANGRGGTLWYAYGAEDTSTTLGASTPGSGNNAFAVDAS
                     TSGPCNSGGSLKVSSPGNEGTNGWGVGFGVNMTPDIGDTKKKSQYDAKAAGYTGVGFF
                     MKCSSETDFAFVKLVDAPNDADIQNPVCSYSTAPICNQYGQKNATLLTDWTYHRVYFS
                     EALQDWDSTTLSTSQMAANALTAFQVQINTRYTRDGSARVKNPFSCWIDDVHFLKEPA
                     PSSNSNVAPKTCTSDAAASAPGGYTVKNNQIVDCKSGSPKLFKGIARPSFEWDRAGWN
                     VSYEDVQKIANWKANVVRFSLNQSFWLDSAKGALYQAYVDRAIKWSLALGMDVILDLH
                     WLTNAQTNSSDAMSATFWGQVAQKYKNDGRVIFELFNEPHDISASQWKTDMQALYNAV
                     RNAGANNLVLVGGLDWAYNLSQVLPANALSGTNIAYVTHPYNFKGNGPSAWDGAFGNL
                     TASYPILSTEFGQANTNPGGALACDANVYTSMLSYFSSKNIGWTAWAWYVDRSVTSAN
                     DTCGFPQLISGYSGNANPAGSVIRTALGN"
     gene            9707..9841
                     /locus_tag="VFQ35_24415"
     CDS             9707..9841
                     /locus_tag="VFQ35_24415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933880.1"
                     /translation="MLRVSILLLRRTDRRGAPPPTLVTHARTYRVSNQFELDRITVRA
                     "
     gene            9873..10334
                     /locus_tag="VFQ35_24420"
     CDS             9873..10334
                     /locus_tag="VFQ35_24420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933881.1"
                     /translation="MGRTQNWRALGNLMVVVHGAESPSVVDWARYVNELRAHVPPVER
                     ILVRTRGGAPDGKQRAAFTEVFRGEQPRVAVMTGSRMVQGVVNAFRIFNPSTSAFALD
                     GWEDVGEFLGLSAEERVRATTLAAELERELSGPRTSLPPSTRAQPVAQRKR"
     gene            complement(10369..11820)
                     /locus_tag="VFQ35_24425"
     CDS             complement(10369..11820)
                     /locus_tag="VFQ35_24425"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9933882.1"
                     /translation="MAGKVSRRGMVGGLSALAVVGFDPHSRKWVASAAAATTFEDLPP
                     LQGALYLDDAHRAAAADDFGHLVHQSPVAVLVPGSVDDIVAMVRYARRHRIGVAMRGQ
                     GHAQYGQSQVAGGLVVDSSSLSAIHSISPSAARVDAGVRWSDLVVAAAERGLTPPVLT
                     DYLELSVGGTLSVGGIGGTMQRFGVQGDNVLELEVVTGEGELVTCSRHHRRELFDAVT
                     SGLGQFALIVRASVRLVPAPAMARVYNLFYDDVATFVRDQLRLVHEARFEYLEGQVVS
                     TEAGGFRYMIEAASLFTTPCSPDEAKLLRGLNDDTATRQKTEVPYLDFAFRLAPTIEF
                     LKSIGVWGFPHPWLSLFVPASRAASYVGNVVADLTTADTGQGPILFYPVLAARVQRPF
                     FRLPNEPVSFAFNILRTASPDPAVQRAMVASNRTLYEAAVAIGGTRYASGAIPMDAAD
                     WRRHFGVSWRDFERAKRRFDPSNILTPGAGIFG"
     gene            complement(12010..>12411)
                     /locus_tag="VFQ35_24430"
     CDS             complement(12010..>12411)
                     /locus_tag="VFQ35_24430"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9933883.1"
                     /translation="VGNFVIAALLALCHRPLLSTLTSDQAIFSLAGPLFVLGVFVEPS
                     RAVNIVAGGALRSSGDARYVAFVGSTMMWVFGVPVCFLFGKWLGWGLTGIWVGLGLDE
                     LTRGIVNYRRWQKGRWQDFRVSVRPQAPART"
BASE COUNT         2018 a         4060 c         4097 g         2236 t
ORIGIN      
        1 gggtttcgac ccgcctcgtg tggctgctcg cctggctgtg cttcgtcctc ttccagggtc
       61 cgttcacgat cgcggccagt cagctcatgt tttctcggtc ggtcgcgctg cgctccgtgc
      121 tcgctcagtt tggacgcggc gtactgcggt acagtgtcgg gaaggtcatg gggggaatgc
      181 tgctcgcggg aggcgcggcg ctcgtgctgc tggtgccggc ggtggtggcg cgcgtgctgt
      241 accttggcga ggtcacgctg gtcgaaggaa ccggcgtcgc cgcttcgtat cggcgcagca
      301 cgcgtctcac gaagaatcga gtcggtgctg cgctcgcggt cgcgctcggc ctaatcgggc
      361 tcgccttcgg cttcgttgtc tgcgcgcacg tgctgatcga ggcggttgcg gagaatttgc
      421 tggggctcgg ctctgtctcg gcgttctggg agaacggccc gaacccttgg tcttgcatgg
      481 gccttttgtg tgcggcgccg ttcgtcgcca ccgcgcgttt cctcgcctat atcgactgtc
      541 gtacccggcg cgaggcgtgg gacgtgcagg tggacttcat gcgactcgcg gcagccgtaa
      601 agaggtcggc gtgaggctcg cctttgcgcg gcgctcgtgc atggtttccg tccgccgttc
      661 gcgactcgcc ttggggttcg cggcgctcgc cgcggcgctc tcgtttgcgg taccagcgtc
      721 tgcagaggaa gggagctcgg agccgccgcg cgcgccgcta gacgtcgacg cgcgcgagag
      781 gttggagcgc gatttgtcgc gcctcgtcgc cgggcctgag ctcgcattct gtcacgatgc
      841 gaccgaagcg atgcggcgag cgaaccgcga agtatgtcgg gtcgtgctca ccaacgcgcg
      901 gcagcgctgt ccggcgatgg cgcgggcttg ttcggcccat tttgcggagc tcgaaagcaa
      961 cggctccgac gaagcgcgct tcggacaggt gccgggcgcg ctcgggagca ttctcttttg
     1021 gggagcgctg gtgctcctcg gtgccgctgt cttgcgcttt ctctggccct tgttggagtc
     1081 gctcgccccg gcgcccgacg tcgttcagaa gccgagcgcg ggaccctctc caaccgagcc
     1141 gaccacgccg cgtcctccgc aggaatcgga tgtcgagcgc ctgctcgcgc aagccgagcg
     1201 cgcgcgcaac gagggccgct tcgaacacgc ggtcgcggcg ctgtacggcg cgctcgtcca
     1261 tttcctgggc cgagagcagc tgataacggt cgaacccgct cgcacgaacg gcgactatct
     1321 gcgcgagctc tcgccccaac ccgagctcgc cgcttcggtg cgacaggcgt ttcgctcggt
     1381 cgagcgagtg cagttcgggc acagcgaagc aacggcggag ctctgctcgg agctttggag
     1441 ccgaatcctg cccatcgtaa aaggtggcgt cgcgctgctc gctttggtgc tgttgccgca
     1501 cacgttgatc gggtgtggcc cgcgcgcttc cctgaacccc gaatctttgc cgaacgggca
     1561 ccacctgctg acgaagctcc tgcgcgagag gggcagggag gttcgccacc tggcgctcag
     1621 cactggcgaa gtcgacgacg acgtttcgca gctcctcgtg ttcggtcgcg acacctctcc
     1681 cgaggtgtgg tccaccgtgc tcgcgtgggc ggaggagggc ggcagcgtga tcgtgaccga
     1741 gccgagcgaa gagctcacgc gcgcgctgca cgtcgaccgc gaaggttcgt ttgcgtgtac
     1801 gcacgcgctc gagttggcgc cccgagcact cgcgcaatcg ctcacggtga tcgatgcggg
     1861 tgcgcgccgt ctgcggctgc cggacgcgcc tggcagcaag agcgcgccgc gcgtcgaagc
     1921 gagctgcgac gggagcgccc gcatcgcgac cttccattac ggcgagggct acgtgaccgt
     1981 gctcgccaac ccggagctgt tcggcaacat ttcactgacc gtcggtgaca acgccgcgtt
     2041 cgtcctcgag ctcttcgggc cgccccgagg agcattgggg ctcgtgggct cgtggacggg
     2101 gggcggagtg acgtctccgc tacgggcgct gcagaatgcg gggctgagcg cgctcatgct
     2161 cgagcttctg gtcgtgctcc tcgtgttctt ctggcatcgc ggcgcggcgt tcggaatacc
     2221 ccgggacccg agcgacctcg cgcgccgcgc gtttgccgat cacgttcgcg cgctcggaag
     2281 aagctatgcg cgagccaagg cgaaccgtca cgcgctctcg cagtatgcgg gctttgcgct
     2341 cgaagtactg cgcgagcgct tcggccgcgg agccccgctc agcctcatcg atctggcggg
     2401 cgaggtcgcg cgccgcaccg gtgagcgcga aggtgaagtg atgcgcctcc tgaccgaagc
     2461 ccacgaagcc gcgcaagacc ccggccccga ccacgggcac cccgcagacc tgcaaacgat
     2521 cgagaagctc gagaccctgc tctatccaag tggaggaccc cagtgaacgt ggaagaagtc
     2581 agcgcgacgc tcaagcggct atctgccgaa atcgcgcgag tgctggtagg ccaagacact
     2641 ctaatcgaac acacgttgat cgggctgctc gcgcgtggcc acctcttgat cgagggagtg
     2701 ccggggctcg gcaagacgct gctcgttcgc tgtctcggtc gcgtgctcgg cctctcgttc
     2761 cggcgtatcc agttcacgcc cgatttgatg ccgagcgacg tcaccggcag caacgtctac
     2821 agcgaggcgg agggtcgctt ctcgttcgtg ccggggccgg tcttcgcgca gctcgtgctg
     2881 gccgacgaga tcaaccgcgc tcccgcgaag actcagtcgg cgctcctcga agccatgcaa
     2941 gatcgcagcg tcaccgtcga cggtgagacg cgcgctcttc ctgcgccgtt cctcgtgctc
     3001 gcgacccaga acccggtcga gtcgcaaggt acgtatccgc ttccggaggc gcagctcgat
     3061 cgcttcttgt tgaagctcga ggtgcgccac ccgacgcgcc aggcggaagc aaatatgctc
     3121 aagaactact tggccggttt cgatccgacc gatctcggcg cgctcacggt gctgagcagc
     3181 gagtcgcacg tgctggagat gcagcgagca ctcggcgagg tgcgcgtcgc gagcgagatc
     3241 ctcgattaca tcaccgacat cgtgcagcgc acgcggtcgc acccggcgct cacgctcggg
     3301 gcctcgccgc gcgcttcgat cgcgcttttg tgcgtcgccc gggctcgcgc gctgtgggag
     3361 ggccgcgact tcgtcactcc cgacgacgtc aagagctacg ctcacgccgt gttgcgacac
     3421 cggttgctgc tccagcccga cgccgagctc gaaggcacca ccgccgatca ttgtatcgat
     3481 ggcatcctgc gcgatacggt cgtgccgggg acgagcgcgt aaccatgccg ctcgtcccgt
     3541 cgcgcgtgct cgtttggtcg tcggcggtgc ccctcgtgct cacggcgctc gccctgctcg
     3601 acgagcgctt tcgagggctt gccatgtggg tgaacgctgc gctcgtcgtg ctcgcgagcc
     3661 tcgatgcgct gttcgcgcgg cgcgcgctcg tggccgtcga acgcttcgct ccggacgtga
     3721 tgtcgctcaa gcggcggaac gaggtgcgcc tgttcgttcg ctcgagctcg cgccggaagt
     3781 tcgtcgtgag agttcgtgac gacctgttcg agggcgcggc cgcagccgac ttaccgctgc
     3841 ggataacgct cgaaccgcgc agcggtcgca acgcctcgta ccacgtcgag ccatcgcagc
     3901 gaggcgcgtt cgagctcggc gaccacaacg tgcgctatcc ctcgccgctc ggcttctggg
     3961 accggcagct tcgtcttcga gcccggaccg ccgtcaaagt ctacccggat ttgcgccaaa
     4021 tccagatgtt cgatcagctg gcgcgcgaga gccgtgagct cgagtgggtt cgctcctcga
     4081 agcgcaaggg cggcgaaacc gagttctctc gtttgcgtga atacgtggcc ggcgacgagt
     4141 accgcgccgt cgactggaag gccaccgcgc ggcggcagca gctcacggtg cgcgagtatc
     4201 agctcgagag caatcagaac atcgtgttca tgctcgacgg cggccgaatg atgaccggaa
     4261 tcgagagcgg gctctcgcgc ttcgatcacg cactcaacgc aaccttgatg ctcgggcacg
     4321 tcgcggcgcg cgggggcgat cgcgtcggtg ttctcggctt cgacgaagca gtgcgagcgt
     4381 tcgtggctcc cgccagcggt ccgcgcgcgg tgcgcaggct aatccaggca acgtacgatc
     4441 tctatccacg cttcgtcgac gcagactacg acgccgcgtt cggctacttg agccagcgtc
     4501 tccgcgctcg cgcgctcgtc gtgatctttt cgcacgtgtt cgacgcctcg gtcgcgcaaa
     4561 ccctgcttcg gcgagttcga gcggttcgaa cccggcacct cccgctcgtg gtcatgtttc
     4621 gcgatcgcga cgtcgagaag ctgctcgagc cagggtctgc cgagggcgcg gaccatttca
     4681 cgaaggccgc cgccgccgag gtcctgcgct tccagaagtc ggtcatgcgc agcatggtgc
     4741 gcgcgggtgc cttgctcctc gagaccgacg ccgcgagcct taccgggaag ctcgtcagcc
     4801 gctacctcga aatcaaggct cgcaatcagc tgtaggggcg tggctagttt tcgcgcaagc
     4861 tactgagcgt gacgacgcgc gtcccggacc aacgatcgtg ccaacccacc gggctcctgc
     4921 tcaagtacga aaagggctcg aatggaacga agcgcgcgaa ggtgcgcccg aggatttgcg
     4981 tgaacctcgg gcttccgccg ttcgctgcga ccacgcgcgt cttcgttatc agtttgccga
     5041 gagtcgctcg aaaggcggtc tctccgacca ggtagtaacc gaagaagaac gtgacgccga
     5101 gaatcccgcc aaccgcaggc ggcatctcgg ggatgatcat cgggagggcc gcgacgatac
     5161 ggctcgcgat gctgtccacg acgaaattgg caaagcgttg gccctgcgac gcaacgacga
     5221 cctcctgagc tgagctcggc gcgggctcgt cgtcagcttc gggcggcgcg tacgggttct
     5281 cgagttggct catggggtgt gagtccgagg gagaatcgat gctctcgcat tcggtgggtc
     5341 gtcggcaagc tctccgaggc tcgagcgcac ttcgaagttt tgactatacg acccggcttc
     5401 cgcacctcgg gcggcggtgg tgcgaccttc gccatcccgc tgctcgaaat ctgcctcttc
     5461 aggtcgcacc gaggtatgtt gagggcgcat gcgaggagcg gcggtggttt ggttggtgtt
     5521 gatatcgtcc ggctgcagtg gcggcgagga taccgctagc gcgagcgcaa tctggtgtga
     5581 cggagtgtgc tcggcggttt atcgctgcgg ctcgactgat tccgtctctt cgtgcagctt
     5641 tcgctgcagg gatgcagagc ccagcctcga agacatcagc gtcgaaggag cccggcccct
     5701 tgcggcctgc ctccaagcaa tgagttgcgc ggacctcttg agctccccgg agcagtggaa
     5761 cgcggcctac gacgcatgct gggaacaagc caaggcgcgc gttccggtca cgaagagcgt
     5821 gcgcacattt tgcgaagcgt acaccttggc cgagttcgac tgtcgctact ggtacgcgac
     5881 cgaagaatgc gaacgcgact tcggcatgtg gagcgagcgc atacggaaca cggtcgcggc
     5941 gtgcgccatg aacccttcgt gcagcgaggc cgaagaatgc gtcgctgcgg tcttcaggac
     6001 ccaatgaaaa gtatcctgtt cgtattggtt ccgctggcca ttggttgcag cagcgaagac
     6061 gacggagcgc tcggcgctcc gccgcttcac ccggtcccgg gctgcgaggg gatcgagccg
     6121 acgagctgtg acgttaggct tcctgagtgc cagagccgtt tgctcaggtt gacggcctgc
     6181 ctgcgcggcg aggacgtgat ggtaccgccg ccgatccgcg tggtgagcga agccgatttt
     6241 acgagcgaat tgacggcaca gtttgcagaa cagatgcagg acccgaacag cggtcacgtc
     6301 gaggccgggc tcaacatgct cggcatggtc gagctgggcg cgctgaccgc gcaggctcgg
     6361 gctgaagact acgcgaagtc catcgccgga ctgtacaacg gagagaccaa ggacatcgcc
     6421 atcatcgatc acggcgactc tctcgccgcg gaacgagcga gccccttgct ggtgcacgag
     6481 ttcgttcacg cgcttcagga tcgcgatctg gggctcgacg cgttcaaagc tcaggcgacg
     6541 tcgaacgatg acacctgggc gaaagtcgcg gtgatcgagg gcgaggcgca gctgcacgag
     6601 atgcgttatg cggcagcgat gctcgggctc gactccgaca aagtcgactg gaaagcgcgc
     6661 ttccaaaacg cgctcgcgca cgacgagcag ctcttgctga aggagaagtc gccgtacctc
     6721 gcgacgtaca agtatttccc gtacgaatgg ggcggccgct acatgtactt cacgtgggcg
     6781 gcggggggga tgagcgccgt gaacggccgg tttgcgcgtc cgccgtcgac gacgcacacg
     6841 ctcctcgcga gcacgatgat ggccatcgat tccgagatca caccggaaac gctcacgcct
     6901 ccgccgccat ccgccgagtg gacccaggtg ctcgcaacga ccctgggcgc gtggggcgtc
     6961 tacctggcat tccagaaggc ggccccgacg ctcgatgcgg cgcgggcgat cgcgctggct
     7021 tggcgcggcg actccctcgc cgtgtacgca ccgagcgaat ctggctccca taccgcagtc
     7081 gtctggagcc tcgagctcgc ggacgaaggc gctgcagccg cggtcgcgca aatcgccccg
     7141 gcgctcgctg cgaacgtacg ccaggcaggc acgcggatcg tcatcgcgaa gacggacggc
     7201 tccggggctc tcgattgggc gttttagttc gtcgggtccg ctgcgggctc cgcgaaaggc
     7261 tccgaaatcg gtccttctaa ttaccgagcg cggtgcggat gacgctgccc gcgggattcg
     7321 cgtttccgct gtagccgctg atcagctgcg gaaagccgca ggtgtcgttg gcgctcgtca
     7381 ccgaacgatc gacgtaccaa gcccaggcgg tccaaccgat gttcttcgac gagaagtagc
     7441 tcagcatcga cgtatagacg ttggcgtcac acgcgagcgc gccgcccggg ttcgtgttcg
     7501 cttgtccgaa ttcggtggaa aggatgggat aacttgcggt gaggttgcca aaggcgccgt
     7561 cccacgcgct tgggccgttg cccttgaagt tgtaggggtg cgtcacgtaa gcgatgttgg
     7621 tgccgctcaa cgcgttggcg ggcaacactt gcgagaggtt gtaggcccag tcgagcccgc
     7681 ccactagcac caggttgttg gcgccagcgt tgcggaccgc attgtagagc gcctgcatat
     7741 ccgtcttcca ttgggaggca gagatgtcgt gtggctcgtt gaacagctcg aagatgacgc
     7801 gcccgtcgtt cttgtacttt tgtgccacct gaccccaaaa cgtcgccgac atcgcgtcgg
     7861 agctgttggt ctgcgcgttc gtgagccagt gcaaatccaa gatgacgtcc atgccgagcg
     7921 cgagtgacca cttgatggcg cgatcgacgt aggcttggta gagcgcacct ttggctgaat
     7981 cgagccaaaa ggactgattt aggctgaaac gaacgacgtt ggccttccag ttcgcgattt
     8041 tctggacgtc ttcgtagctc acgttccagc cagctcggtc ccactcgaac gacggacggg
     8101 cgatgccttt gaagagcttc ggcgacccgc tcttgcagtc gacgatttga ttgttcttca
     8161 ccgtgtagcc gccgggcgcg ctcgcggccg cgtcgctcgt gcacgtcttc ggagccacgt
     8221 tcgagttgct gctcggcgcc ggctctttca agaaatgcac gtcgtcgatc cagcagctga
     8281 atgggttctt cacgcgcgcg gagccgtccc gcgtgtaacg cgtattgatt tgaacctgga
     8341 acgcggtgag cgcgttcgcc gccatctgtg acgtagagag ggtggtggag tcccagtctt
     8401 gcagtgcctc gctgaagtac acgcggtggt acgtccagtc ggtgaggagc gtcgcgttct
     8461 tctggccgta ctggttgcaa atcggtgcgg tcgagtagga gcagaccgga ttttgaatgt
     8521 cggcgtcgtt gggcgcgtcg acgagcttca cgaaggcgaa gtccgtctcc gagctgcatt
     8581 tcatgaagaa gccgacgccc gtgtagcccg cggcttttgc gtcgtattgg ctctttttct
     8641 tggtgtcgcc gatgtccggc gtcatgttga cgccgaagcc cacgccccag ccgttcgtgc
     8701 cttcgttgcc gggcgacgag accttgagcg agccgccgct gttgcacggg ccgctcgtgc
     8761 tcgcatcgac cgcgaaagcg ttgttgccgc tgcccggggt cgaagcgccg agcgtcgtgc
     8821 tcgtgtcttc cgcgccgtaa gcgtaccaga gcgtgccgcc gcgaccgttt gccgcgtaca
     8881 tcgtcggcgt gttctgtcga aaatccgaga tgacgtcggc cgcggcgacg ttgacctgcg
     8941 tgccgccgac gttgcaccga aggtccaccg ctgcctgtcc ggcgccagcc gcgcccgcgt
     9001 ttccagcgag cgggccgccg gcagcgccag cgttcgcggt gcttccgccc gagccacccg
     9061 cgccgcttcc tcccgcgttg cagtttgcgc cggtaacgca cgagttcccg cacagtgttt
     9121 gaccgctcgg gcagccgcag gtgctcgcgt tgcagacacg cccactcgca caagcgttgt
     9181 tacacgtgcc gcaatgcccc gagttcgttc gaaggtcgac gcactggccg tcgcaagacg
     9241 tcataccact cgagcacgcg ctgcctccgc ccgcccccaa cgcgccgcct ttcgggccgc
     9301 ctccgccggc accaccgccc ccacctgcgc caccttgcct agtcggcgcg ctcgtcccgc
     9361 acccccacag cgccagcagc gtcaacccaa ccaccaaacc cgagaaccgc atcgtcctcc
     9421 gccgagcttt gcacgcgcag cacgcgagac aaaccggatc ggcgaggagc gctcgtagtg
     9481 cccgcgtcca cgctttcccg agcgccgcgc gacggaccga tcctctgttc aggtgccacg
     9541 agacgtcgaa ctcccgaggt cgcggccacc cccgacgatc gcggcgtgcc caggtagcga
     9601 cgtcgcaacc tgggattcat cggcccgaga agtcgtcgtt acctttcgcc tgcagcgcca
     9661 agcgtccgac agaagcgaac gttaagggcc cgcgaatggg taggccatgc tgcgcgtgtc
     9721 gatcctcttg ctgcggcgga cggatcgtcg cggcgctcct ccgcctactt tggttacgca
     9781 cgctcggacg tatcgcgtat cgaaccaatt cgagctcgac cggatcacgg tgcgcgcttg
     9841 acccatccga tgcgatcgcg aaaagtttgt gaatgggccg cacccagaat tggcgcgcat
     9901 tgggcaactt gatggtggtc gtgcacggcg cggagtcgcc ctccgtcgtc gattgggcac
     9961 gatacgtcaa tgaacttcgc gcccatgttc cgccggtgga gcgcattctg gtgcgcacgc
    10021 gtggcggtgc cccggacggt aaacaacgcg cagcgttcac cgaggttttt cgcggcgagc
    10081 agccgcgagt cgcggtcatg accgggagcc gaatggtgca gggcgtggtc aacgctttcc
    10141 gcatcttcaa tccgagtacg agcgccttcg cgctcgatgg ttgggaagac gtcggtgagt
    10201 tcttgggcct atccgcggag gagcgcgtgc gcgcgacgac cctggccgcc gagctcgagc
    10261 gtgagctcag cggtccgcga acctccctgc cgccctccac gcgtgcccaa cccgtcgctc
    10321 agcgcaaacg ctaggcggga cgggagccga cgcgagcccc cgcaacgctc aaccgaagat
    10381 cccagcgccc ggcgtgagga tgttgctcgg atcgaagcga cgtttggcgc gctcgaagtc
    10441 gcgccagctg accccgaagt gccggcgcca atcggcggcg tccatgggaa tggccccact
    10501 cgcgtagcgc gttccaccga ttgcgactgc ggcctcgtac agcgtgcggt tgctcgccac
    10561 catcgctcgt tgcacggcgg gatcgggcga agccgtccgc aagatgttga aggcgaacga
    10621 gacgggctcg ttcggtaggc ggaagaacgg ccgctgaacc cgcgcggcca atacggggta
    10681 aaacaggatc ggtccttggc ccgtgtccgc cgtggtcagg tcggccacga cgttgccgac
    10741 gtagcttgca gctctcgatg cggggacgaa caagctgagc cacgggtgcg gaaagcccca
    10801 cacgccgatc gacttcagga actcgatggt gggcgcgagc cggaacgcaa agtccagata
    10861 gggcacctcg gtcttctgac gagtggccgt atcgtcgttc aacccgcgca gcagtttggc
    10921 ttcgtcgggc gagcacgggg tggtgaatag gctcgccgct tcgatcatgt agcgaaagcc
    10981 ccccgcttcc gtgctcacca cttgcccctc gaggtattcg aagcgcgcct cgtgaacgag
    11041 ccgtagctga tcgcgaacga aggtcgcgac gtcgtcgtaa aagaggttgt acactcgggc
    11101 catcgccggt gcgggaacga ggcgcacgct ggcccgcacg atgagcgcga attggcccaa
    11161 tccgctggtc actgcgtcga acagctcgcg tcggtggtgg cgcgaacacg ttacgagctc
    11221 tccttcaccg gtcaccacct cgagctcgag cacgttgtct ccctgcacac cgaagcgctg
    11281 catcgtgccg ccgatgccgc cgaccgaaag agtgcctccg accgagagct ccaagtagtc
    11341 cgtcagcacg ggtggagtca gtcctcgttc ggctgccgcg acgaccaggt cgctccagcg
    11401 cacgcccgcg tcgacgcgcg cagcgctcgg cgaaatcgag tgaatcgccg acaggctgct
    11461 cgagtcgacg accagccctc ccgcgacctg agattggccg tactgcgcgt ggccctggcc
    11521 gcgcatggct accccaatcc gatgccgtct cgcgtagcgc accattgcga cgatgtcgtc
    11581 gacggagccg ggaacgagca cggccacggg actttggtga accaagtgcc cgaagtcgtc
    11641 ggcggccgcg gcgcggtgcg cgtcatccaa atacaaagcg ccttgcagcg gtggcaggtc
    11701 ttcgaacgtc gtcgcggccg ctgcgctcgc gacccatttg cgcgaatgcg gatcgaaccc
    11761 caccaccgcg agagcgctca gccctccgac catgccacgt cgcgaaactt tcccagccat
    11821 ctccgacctc cctctcgagc cgttgcttcg gcgcggccag cgtaacccgc gactttgcag
    11881 gttggctgga ccaattgctc tgtctagtcg accgaggccc gtgggtagcc gaattccgga
    11941 gacctccacg tttccggacg cttccgcgtc acccgacgcg acacgcggtc ggtgcgggaa
    12001 ccggtcgcgt caggtgcgcg cgggcgcttg cgggcgcacg gagacgcgga aatcttgcca
    12061 gcgccctttt tgccagcgcc ggtagttcac gattcctcgc gtgagctcgt cgaggccgag
    12121 ccccacccag attccggtca ggccccagcc gagccatttg ccgaacaaga agcagacggg
    12181 cacgccgaac acccacatca tggtgctacc cacgaacgcc acgtagcgcg cgtcgccgga
    12241 cgagcgcagc gcgccgccgg ccacgatgtt cacggcgcgg ctcggttcga cgaagacgcc
    12301 gagcacgaac aagggccccg caagggaaaa aatggcttgg tccgaggtca gcgtcgaaag
    12361 cagtgggcgg tgacacaggg ctagcagagc cgcaatcacg aagtttccca c
//
LOCUS       DASTKS010001149         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_182736, whole genome shotgun sequence.
ACCESSION   DASTKS010001149 DASTKS010000000
VERSION     DASTKS010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182736"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VFQ35_24435"
     CDS             <1..1542
                     /locus_tag="VFQ35_24435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933884.1"
                     /translation="GCSSDASPGSSSERPSDGSAGAFNGSGGSSNGIPNPNGGSAGRA
                     PEVENESSYLAPVATGKYLWSANPLSGRVALIDAETLSVKLQVAGNRPTSVLGLESAG
                     QFGALVLNDLSNDATLLRIDDQGELRRVRSFQTQPDANAWSRSSSGKWAVAWTDATQK
                     SGVGPLDLFQDITLFSLEVGSEASFRLSVGSRPSRFFFDGAERYLYVVTEEGISVIEL
                     GARPAVSGVVALTTGFVAERSQADVSFAPDGSYAVVRASVGTKVATLSLPDGAREEVD
                     LGGIVTDLDLTPDGARAFAVIGATAQVIEVPIPIAGRAPSSFRSVVLTGEAIGAVALN
                     ADASAALVYSTVTGSTRVALLDVRPGANPLGYKVQDLISPVTALFAAPDPRFAVTFQS
                     APAGSKRVGAFSLLSMQGTRVPKIVGTDAAPAQIAYAGTTALVTVRSDARSTFGAYVI
                     ALENQQVDFVPLQSPPLAAGVVAAAERAYIAQSHPEGRITFVSLADGQLQTLTGFELA
                     ARIVQ"
     gene            1561..3168
                     /locus_tag="VFQ35_24440"
     CDS             1561..3168
                     /locus_tag="VFQ35_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933885.1"
                     /translation="MSYVELRSKRALAAMVAALGLTGCGDRNPAWDTPFVRGQSVGLS
                     GAVAIVDGNRNEAMMLTSPSEGNLSVSRLKVGKNVALALPSADRERLFVLSRGDERRL
                     HERDEKPRLTVIEGGVEPSIFKTYELGDPAQKLVLDPEGRWAIVYDTGGVVVNSNELV
                     LVDLEASEPSAALHSTTLHSTGGQPERFTFTSPLTLPSGERHQLLVVETNQDLSVLDL
                     EQPDKPEVRVSLPKARAGGTAHPTLVAFHDDASASGTGSYLAVGFENDPSVLTLKLGD
                     RTDKSVSGISVVPNLLDSGAIPSTIDFVETDSGLRLAALVPDQKAAVLFDPTTSKSER
                     VEFDKPYTGIARITGVVDDTSGGGDVALLYSSRDATIAFWRLGSASTTPYASYDSYGV
                     DTRVTTVLDIPGEKYGYLKLLTGANTAEFFLLDLRRRESYPMKTLAGFNLSLSPDGMR
                     AWAFPQHGDKLAELTFDPHHPTSFSVERPIENVFDIARKDGGRSALVLHVVDGAEQDL
                     GVTLFDAEHPDSAHTRFFSGLISEGLQ"
     gene            3165..>3822
                     /locus_tag="VFQ35_24445"
     CDS             3165..>3822
                     /locus_tag="VFQ35_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933886.1"
                     /translation="MNTLSSIALLAALATSAISAHAAEATPPEPQSPPNTAQPPTSTP
                     TPPDDAAAPSGASPVAPPAPAPVLVQTPAKPADAVPPPAAAARPSETPFDDTGVPDDG
                     SLGTHQSHFWLTVGERTTFVKNRGFDVFANDDVLAQFNLAFGRVLFHEGPWSLASSLS
                     WEVGGRSSDVRGAKTTLQVNRFTLNPEARYHFFRRLYAFGRVGAGAALLSSTLQDPVG
                     G"
BASE COUNT          649 a         1281 c         1243 g          649 t
ORIGIN      
        1 gggtgcagca gcgacgcgag ccccggcagc tcgagtgagc ggccaagtga cggcagcgcg
       61 ggagcgttca acggcagcgg tggcagctcg aacggcatcc cgaatccgaa cggcggctcg
      121 gcgggtcgag ctcccgaagt cgaaaacgag agcagctatc tcgcgccggt cgcgacgggc
      181 aagtacttgt ggagcgcgaa tccgctctcg ggtcgcgttg cgctcatcga cgccgagacg
      241 ctctcggtaa agcttcaggt tgccggaaat cgtccgacga gcgtgctcgg gctcgagagc
      301 gctgggcagt tcggcgcgct cgtgctcaac gacctgagca acgatgcgac gttgctgcgc
      361 atcgacgacc agggggagct gcggcgcgtt cgctcttttc agacgcagcc ggacgcgaac
      421 gcctggagcc gcagctcgag cgggaaatgg gccgtcgcat ggacggacgc tacgcagaag
      481 agcggcgtgg gcccgctcga cttgttccag gacatcacgc tgttttcgct cgaggtcggc
      541 agtgaagcat cgttccgcct cagcgtcggt tcgcggccgt ctcggttttt cttcgacggc
      601 gccgagcgat acctctacgt cgtgaccgaa gaggggatca gcgtcatcga gctcggggct
      661 cggcccgccg tgagcggagt ggttgcgctg acgacgggct tcgtcgccga gcgtagccaa
      721 gccgacgtga gcttcgcgcc ggacggcagt tatgccgtgg tgcgcgcttc ggtgggaacc
      781 aaggtggcga cgctctcgtt gcccgacgga gcgcgggagg aggtcgatct gggaggtatc
      841 gtcaccgatc tcgatttgac cccggatggc gcgcgagcgt tcgcggtgat cggcgcgacg
      901 gcgcaggtca tcgaggtgcc gatccctatc gccggtcgcg ctccgagtag ttttcgcagc
      961 gtcgtgctca cgggcgaagc cattggcgcg gtcgcgttga acgccgacgc cagcgccgcg
     1021 ctcgtgtact ccacggtgac cggatcgaca cgcgtcgctc tgctcgacgt gcgcccaggt
     1081 gcaaatccgc tcggctacaa ggtccaagat ctgatttccc cggtcacggc cctgttcgcg
     1141 gcgccggatc cgcgcttcgc ggtgactttc cagtcggcac ccgctgggag caagcgcgtg
     1201 ggcgctttca gcttgctctc gatgcaggga acgcgagtgc cgaagatcgt cggcaccgac
     1261 gcggccccgg cccaaattgc ctacgcggga accacggcgc tcgtgacggt gcgaagcgac
     1321 gcgcgctcga ccttcggcgc gtacgtcatc gcgctcgaga atcaacaagt cgatttcgtg
     1381 ccgctgcaga gcccgccgct cgccgcgggc gtggtcgccg ccgcagagcg cgcttacatc
     1441 gctcaatctc accccgaagg acgcatcacc ttcgtttctc tcgccgacgg gcaactccaa
     1501 acgctgaccg gcttcgagct ggcggcgcgc atcgttcaat agtcgcactc aggtatcttc
     1561 atgtcttacg tcgaacttcg ttcgaagcgc gccctcgccg cgatggttgc ggcgctgggg
     1621 ctcaccggct gcggagatcg caacccagcc tgggacacgc cgttcgtgcg cggacagagc
     1681 gtggggctct cgggagccgt cgcgatagtc gacggcaatc ggaacgaggc catgatgttg
     1741 acctctccca gtgagggcaa cctctccgtt tcgcggctga aagtgggcaa gaacgtcgcc
     1801 ctggccctgc cgtccgcgga tcgcgagcgg ctcttcgtgc tctcgcgtgg cgacgaacga
     1861 cggctgcacg agcgcgacga aaagccccgg ctcaccgtca tcgaaggcgg agtcgagccg
     1921 agcatcttca agacttacga gctcggcgac cccgcccaaa agctcgtgct cgaccccgag
     1981 ggacgctggg cgatcgtcta cgacacgggc ggcgtcgtcg taaactccaa cgagctcgtt
     2041 ttggtcgatc tcgaagccag cgagccgagc gccgcgttgc actcgacgac tttgcacagc
     2101 accggcgggc aacccgagcg cttcaccttc acgtcgccgc tcacgttgcc gagcggcgag
     2161 cgacaccagc tgctcgtcgt cgaaaccaac caagacctct cggtgctcga cctcgaacaa
     2221 ccggacaagc ccgaagttcg cgtcagctta ccgaaggcgc gtgccggcgg tacggcgcat
     2281 cccaccctgg tcgcattcca cgacgacgcg agcgcgagcg ggactggttc gtacctcgcg
     2341 gtggggttcg aaaacgaccc gagcgtgctc actttgaagc tcggcgacag aacggacaag
     2401 agcgtgagcg gcatctcggt cgttccgaac ttgctcgaca gcggggcgat cccgagcacc
     2461 atcgacttcg tcgaaaccga tagcggcctt cgactcgcag cgctggtccc cgatcagaag
     2521 gcggcggtgt tgttcgatcc tacgacgtcg aagagcgagc gcgtcgagtt cgacaagccg
     2581 tacaccggaa tcgcgcgcat cacgggcgtc gtcgacgaca cttccggcgg gggcgacgtc
     2641 gcgctcctct atagctcgag ggacgcgacg atcgccttct ggcgtctggg cagcgccagc
     2701 acgacccctt acgcgagcta cgactcgtac ggcgtcgata ctcgagtcac gaccgtcctc
     2761 gacattcccg gcgagaaata cgggtatttg aagctcctca ccggcgccaa caccgccgag
     2821 ttcttcctct tggatctgcg gcgccgggaa agctatccga tgaagacgct cgctggcttc
     2881 aacctgagcc tgtctccgga cgggatgcgc gcctgggcgt ttcctcagca cggtgacaag
     2941 ctcgcggagc tcacgttcga tccgcatcac ccgacttcgt tctcggtgga gcgcccgatc
     3001 gagaacgtct tcgacatcgc gcgcaaggac ggcgggcgta gcgcgctcgt gctgcacgtc
     3061 gtggacggcg cggagcaaga tttgggtgtc acgctgttcg acgccgagca tccggactcg
     3121 gcgcacacgc gcttcttcag cgggctgatc tcggagggtc tacaatgaac acgctatcgt
     3181 cgatcgcgtt gctggccgcg ctcgcgactt ccgcgatcag cgcgcacgcg gccgaagcga
     3241 ctcccccaga gccgcaatca ccaccgaaca cggcccagcc gccgacgtcg acgccaaccc
     3301 cgcccgacga cgctgccgcg ccgagcggcg cgagcccggt cgcgccccca gcgccggcgc
     3361 cggtgctcgt ccaaacccca gcaaaaccag ccgatgcggt gcctccgccc gcggctgcgg
     3421 cgcgccccag cgaaacgccg ttcgatgaca ccggtgtccc cgacgatggc agcctcggca
     3481 ctcaccagag tcacttctgg ttgacggttg gcgaacgcac caccttcgta aagaatcgcg
     3541 gcttcgacgt gttcgcgaac gatgacgtac tcgcgcagtt caacctcgct ttcggtcgcg
     3601 tgttgttcca cgaaggacct tggtcgctcg cttcgtctct ttcgtgggag gtcggaggcc
     3661 gcagctccga cgtacgcggt gcgaagacga cgctgcaggt gaaccgcttc acgctgaacc
     3721 ccgaagctcg ctaccacttc ttccggaggc tctatgcgtt cggtcgcgtt ggcgctggtg
     3781 ccgcgctgct gagcagcacg ctccaagatc cggttggcgg ag
//
LOCUS       DASTKS010001150         6631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_404910, whole genome shotgun sequence.
ACCESSION   DASTKS010001150 DASTKS010000000
VERSION     DASTKS010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404910"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..642)
                     /locus_tag="VFQ35_24450"
     CDS             complement(106..642)
                     /locus_tag="VFQ35_24450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933887.1"
                     /translation="MKVRSLAPAFAALLAATAAHAEPEWNVGVTAAGCLVGDHPKLFE
                     YAAFCGSVGGDVLFLRESTKDFGIGPYASLGTAAFSDLRVSAGARALFPIAEDFPLVA
                     SLGGVLRDGRDPGIEGSLFWGLRSFNFHGSYNIAVGLVLTGTHLFEGPRSNALALGAQ
                     VDGAILALPFLLAMGALK"
     gene            complement(668..1483)
                     /locus_tag="VFQ35_24455"
     CDS             complement(668..1483)
                     /locus_tag="VFQ35_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933888.1"
                     /translation="MTWLRKFTGALGLVTLGALCVPGCADNNSMLFIEGVLAVEASDC
                     IAKAESGALIREGGTLDLSLRSGYRAALLVGSQLTQRGSREQLRTETARLVLKGADIT
                     LQDAYGRNLDLGGNPNPFSTIGTGFVNPAAGTEPGLGVIFVDVIPSVLSDAVSGALNG
                     SGLVLAKIKAFGETLGNQKVESGEFLYPIQICRGCLISYPATSMTGAVGALTCAAPTQ
                     GSSTETLCSLGQDQFVPCTQCLGIPACSDPCQNCTYRAGSPQCTGAAPDECAQ"
     gene            complement(1679..3253)
                     /gene="aroB"
                     /locus_tag="VFQ35_24460"
     CDS             complement(1679..3253)
                     /gene="aroB"
                     /locus_tag="VFQ35_24460"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET9933889.1"
                     /translation="MLRPLLLNGFMGTGKSSVGRLVATRTGRRCVDLDSLVEARAGMG
                     IARFFAERGEPAFRALEREVLLEQLARREPIVIALGGGALVNRETRLRALDEAVVVTL
                     HASADEVLRRCASDDSRPLLAGPDRAARIQELLAARAGSYAETHASVATDGKSLDAVA
                     AEVTAVWKRDPLAVAAGASSYAVDVGAGILTERLPVLAGAPSRIVLVSDETVFGLHGH
                     SAVTALGSPAVVRLPPGEENKHIATVERIWRAALEAHADRKALFVALGGGVVTDMTGF
                     AAATFMRGVRWLGIPTTLLAMVDASVGGKTGVDLMAAKNAVGAFWQPSGVICDVDVLR
                     TETPRAFRSALAEVVKTAIIGDPDLLGAFDRAEELETVRSHELLSDMVRRSVRVKARI
                     VSADERESGLRAVLNLGHTVGHALEAVAGYSRLTHGEAVSLGLVAALRIGVTLGFTEV
                     SLADRVEELLRRIGLPTDVRSEPLDDAVRLIAHDKKRKASKLTFIVARDIGRVETVDL
                     PLAELESAVLGLKRVA"
     gene            3639..5420
                     /locus_tag="VFQ35_24465"
     CDS             3639..5420
                     /locus_tag="VFQ35_24465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9933890.1"
                     /translation="MHDSVLPSARSGAPPSFSSGSDEASDDLVVVGIFAEPDAGELLR
                     RALANSADRVSVALDLAEGLTRVSAEAPDLVFVDVTLGGNAGLAVVHHIRALSPNTAV
                     YAVTRTDRLELGLQAVALGSQGTLIFPLSGDDVLTAVGEVRRRRAELSRRSRFTDPVL
                     RESKAAALCADLLELVDTPSRRSAAERLASVFAERLPARRVLVYFAASAASRQLVRAA
                     VYGESSNEPSFCDELELLRHAKAENLEVIRLALRREQLGLVLVDAPQAEGSLLATASA
                     TATLAVAVVGAREQSSRGAMKDPESSAYTFAYFVDVAGREIDIARRHGRRFALATIVV
                     EGAGREPTSLREPTVDAAERVLSAVRDTDVLARVDANEFYLLLPETGGLGAQQCRRRV
                     LDQLGVFARFEVAMGLANFPHDGSDLSRLLRVAKHRADACSRSVVESFGLHQLSFVEL
                     LDALLGSVVMRSALTPGDLEAPRYIELPAMDATSVAISAVREAARGGETRVVATQHAG
                     ISLGGAVRAEVTRELEGVKLDVVEVSQLAGGENADVLIIIAQQACYLMAGRSEAGLVR
                     AVHATDPLLADLLLRRLGEATLTRLLD"
     gene            5420..>6631
                     /locus_tag="VFQ35_24470"
     CDS             5420..>6631
                     /locus_tag="VFQ35_24470"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9933891.1"
                     /translation="MARTVLLIEPDVDVLGALASKLRSRGLEVWIADGLESAVARVRA
                     GRPDAVLLAIELGVETERALAEALGTPTFTCFRLVDEAEPGRASLLPRSDPDAIARRM
                     HALPTASASIVPEASDFRGDLSQVSIVDLIQLLGANRRSGVLLLQTPLGAGEVRFRDG
                     EVIDALYRRLEGKKALFRLLGEREGTFSFVGGHAGSYIHRIEGPTYALLMDGVRELDE
                     ARELRAALELSEDALVSVHGDAQSGNDAQALVLGMLMSPRTLAELLDDVPALDLDVLQ
                     AVKRLLAAGSIRRISGGTRRIELSDPDRLGVLAALAKRVARPGFRDAGRLAIAAEPGR
                     LLGIAASLLRIAEALPPSEAAPTTPIPHVLATLRLGESVDLDIVGIPLVHAYAPLWSL
                     VLPGCSAVAVLD"
BASE COUNT         1040 a         2182 c         2329 g         1080 t
ORIGIN      
        1 tcgcgttggg ccgcgccgtt gatcgcgagc gccgagtgag tgccgtagtg agggccgccc
       61 agcacggacc cttgggtagg acgcgggtag gcggcgagtt cggggctatt tgagcgcgcc
      121 catcgccagc aggaagggga gggcgaggat ggctccgtcg acctgcgcgc ccagagcgag
      181 cgcgttcgag cgggggcctt cgaagaggtg ggtgccggtc agcaccaagc ccacggcgat
      241 gttgtaagac ccgtgaaagt tgaaacttcg gaggccccag aagagcgagc cttcgatgcc
      301 agggtcacgg ccgtcgcgca gcacgccgcc gagcgaagcg acgaggggga agtcttccgc
      361 tatcggaaag agcgcgcgcg ctccagcgga gacgcggaga tcgctgaagg cggcggtgcc
      421 gagagaggca tatggtccga tcccgaagtc cttcgtgctt tcgcgcagga agagcacgtc
      481 gcctcccacc gacccacaaa atgcggcgta ttcgaagagc ttcgggtgat ctccgacgag
      541 acacccggcg gcggtgaccc cgacgttcca ctctggttcg gcgtgagcgg ccgtcgctgc
      601 gagcagcgcg gcgaacgccg gcgccagcga gcgcaccttc attgtcggcg ctgctcgctg
      661 agtgctttca ctgagcgcac tcgtccggag ctgcgcccgt gcactgcggc gagccagcgc
      721 ggtacgtgca gttttggcag gggtcgctgc aagcggggat gccgaggcac tgggtgcacg
      781 gaacgaactg atcttggccc aagctgcaga gcgtttcggt ggaagagccc tgcgtcggag
      841 cagcgcacgt cagcgctccc accgcgcccg tcatcgaggt cgcgggatag ctgatgaggc
      901 agccgcgaca tatctgaatg gggtagagga actcaccaga ctcgaccttt tgattgccga
      961 gcgtctcacc gaaggctttg atcttggcga gaacgagccc cgaaccattc agcgcgccac
     1021 tcaccgcgtc gctgaggaca gagggaatga catcgacgaa aatgaccccc agaccaggct
     1081 cggtgcctgc cgcggggttc acgaagccgg tgccaatcgt cgaaaatggg ttggggttgc
     1141 cacccaaatc gaggttgcgg ccgtaagcgt cctgcaacgt gatgtcggcg cccttgagga
     1201 cgagacgcgc ggtctcggtg cgcagctgct cgcgcgagcc ccgttgggtc agctggctgc
     1261 ctaccaagag cgcggcgcgg tagccgctgc gaagcgacag gtcgagggtg ccgccttcgc
     1321 ggatgagcgc gccgctctca gccttggcga tgcagtcaga cgcttcgacc gcgaggacgc
     1381 cctcgatgaa gagcatgctg ttgttgtctg cgcacccggg aacgcacagg gcccccagcg
     1441 tgaccaaacc cagagcgcca gtgaatttcc gcagccaagt cattaggggg acctcggtcg
     1501 caacgccgcg cggcgacgcg tctctacagc aacggagtct agcctgcccc ctccggagcg
     1561 ccaaaatagt gggcagggcg gcgcctctca acccacacgc ctcccgaagg cacacctgtg
     1621 cccgaagcat ctactgcggc cattcaggtc aaatgaccct tggacgggcc gggcttattt
     1681 aagcgactcg cttcagcccg agcactgcgg actcgagctc tgccagaggc agatcgacgg
     1741 tttcgacgcg gccgatgtcg cgcgcaacga tgaacgtgag tttggatgcc ttccgcttct
     1801 tgtcgtgcgc gatgaggcga acggcgtcgt cgagtggctc gctgcgaaca tccgtcggca
     1861 gcccaatgcg gcgcaggagc tcctcgacgc ggtcggccaa gctgacttcg gtgaacccga
     1921 gcgtgactcc gatgcgcagc gcggcgacga gcccgaggct caccgcttca ccgtgcgtca
     1981 atcgcgaata gcccgcgacg gcctccaaag cgtggcccac ggtgtgaccg aggttcagca
     2041 cggcgcgaag cccgctctcg cgctcgtcgg cgctcacgat gcgagctttg acccgcacgg
     2101 agcggcgcac catgtcgctg aggagctcgt gggagcgaac ggtttcgagc tcttctgctc
     2161 ggtcgaacgc cccgagcagg tccgggtcgc cgatgatcgc ggttttgacg acctcggcga
     2221 gcgcgctgcg gaaggcgcgc ggcgtctccg ttcgcaggac gtcgacgtcg cagatcaccc
     2281 ccgaaggctg ccagaaagct ccgaccgcgt tcttggccgc catcaaatcg acgccggttt
     2341 tcccccccac cgaagcgtcg accatcgcga gcagagtggt cggaatgccc agccagcgca
     2401 cgcctcgcat gaacgtcgca gcggcaaatc cggtcatatc ggtgacgacc ccacccccga
     2461 gtgcgacgaa gagagccttg cggtcggcgt gcgcttcgag ggcggcgcgc cagatcctct
     2521 ccaccgtcgc gatgtgcttg ttctcctcgc cgggcggaag gcgaacgacg gctggggacc
     2581 cgagcgcggt gacggcgctg tgcccgtgaa gcccgaagac ggtctcgtcg ctgacgagga
     2641 cgatgcggga cggcgcgcca gcgagtaccg gcagacgctc cgtcaggatt cctgcgccga
     2701 cgtcgaccgc gtaggagctc gcacccgccg ccacggcgag cggatcgcgc ttccacacgg
     2761 ccgtgacctc cgccgcgacc gcatcgaggc ttttcccatc ggtggcgacg ctcgcgtgcg
     2821 tctccgcgta gctgccggcg cgcgcggcga gcaactcttg gatgcgcgcg gcgcggtcgg
     2881 gtcctgcgag caacggccgc gaatcgtccg aggcgcagcg gcgcagaact tcgtctgcgc
     2941 tcgcgtgcag cgtaacgact acagcctcgt cgagggcacg caatcgggtc tcgcggttca
     3001 ccagcgcacc gccgccaagc gcgattacga tgggctcgcg gcgcgccaac tgctcgagaa
     3061 gtacttctct ttccagggct cggaatgcgg gctcaccacg ttcggcgaag aaccgcgcga
     3121 tccccatccc ggcgcgcgct tcgacgaggg agtcgaggtc cacgcagcgg cggccggtgc
     3181 gtgttgcgac caggcgaccg accgaggact tcccggttcc catgaatccg ttcaggagga
     3241 gagggcgcag catgaggggc gaagggtgcc aaacgtcgac cgctgacgcc agagccgagc
     3301 gtcgccgcgc gttcccaggc aggagtgttg gtgcgcctcg ggcccccgat gtggaagcga
     3361 tcgtcccaat tcgatggcga ggatgcctcg agggcccgca gcgcgccggg tgagcctcgt
     3421 cccaccccac cgactcgcat tcaggtcatt ccgcttaatc cccgtatgac cgtccagccc
     3481 gttcggggcc cgagaacccc aagggagtga cgcgccaaca cgttcaccgt gggggcgtgt
     3541 cgtttcagcg tcgcgcggac tcgccgactt cgggagtccc acatttgtgt gcggagagag
     3601 cgtcgcgact cgcggttgag atagaattga ggttcgcttt gcacgactcc gtactccctt
     3661 cagctcgttc gggggcgcct ccgagcttct cgagcggcag cgacgaagct tcggacgatc
     3721 tcgtcgtggt cgggatcttc gcggagcccg acgccgggga gctcttgcgg cgcgctctcg
     3781 cgaacagcgc cgaccgcgtc agcgtcgcgc tcgatttggc ggaagggctg acgcgcgtgt
     3841 cggcggaagc gccggacctc gtgttcgtgg acgtgacgct cggaggcaac gcgggcctcg
     3901 cggtggtcca tcacattcga gcgctctcgc cgaacaccgc cgtatacgcc gtgacccgca
     3961 cggatcggct cgagctcggg ctacaagcgg tggcgctcgg gagccagggc acactgattt
     4021 ttccgctctc cggagacgac gtgctcaccg cggtgggtga ggtgcgacgg cgccgcgcgg
     4081 agctctcgcg gcgctcccgc ttcacggatc cggtgctgcg ggagagcaag gcggcggcgc
     4141 tctgtgccga tctgctcgag ctcgtggaca ctccgagccg tcggagtgcc gccgagcgac
     4201 tggcgagcgt gttcgcggag cgcctgccgg cgcgacgcgt gttggtgtat ttcgccgcga
     4261 gtgcggcttc gcgccagctc gtgcgggccg cggtttacgg ggagagcagc aacgaaccgt
     4321 cattttgcga cgagctcgag ctcctgcgcc acgcgaaggc cgagaacctc gaggtgattc
     4381 ggctcgcgct acgccgcgag cagcttggcc tcgtgctcgt cgatgctccg caggcggagg
     4441 gctcgttgct cgcgactgcc tcggctacgg cgacgctcgc ggtcgcggtg gtcggcgctc
     4501 gggagcagtc gagccggggc gcgatgaagg acccggagtc gagcgcctat acgttcgcgt
     4561 acttcgtcga cgtcgccggg cgcgaaatcg acattgcgcg ccgacacggg cgaaggttcg
     4621 cgctcgcgac gatcgtggtc gagggagcag ggcgcgagcc aacgtcgttg cgcgagccga
     4681 ccgtcgacgc cgcggagcgc gtgctgtctg cggtgcgcga cacggacgtc ctcgcgcgcg
     4741 tcgacgccaa cgagttctac ctcttgctgc cggagacggg cggcctcggc gcgcagcagt
     4801 gccggcgccg ggtgctggac cagctcggtg ttttcgcgcg cttcgaagtc gcgatggggc
     4861 tcgccaattt cccccacgat ggctcggatt tgtcgcggct tttgcgggtc gcgaagcatc
     4921 gcgccgacgc ctgctcgcgg tctgtagtgg agagcttcgg gctgcaccag ctttcgttcg
     4981 tcgagttact cgatgcgctt ctcgggagcg tggtgatgcg ctcggcgctg acccccggcg
     5041 acctggaggc gccgcgctac atcgagcttc ccgcaatgga cgcgacgtcg gtcgcgatca
     5101 gcgccgtgcg tgaagcagcg cgcggcggtg agacgcgtgt cgtggcgacg cagcacgctg
     5161 gcatcagcct cggcggcgcc gtccgcgcgg aggtcactcg agagctcgag ggagtgaagc
     5221 tcgacgtcgt cgaggtctca cagctcgcgg gtggagagaa cgcggacgtg ctcatcatca
     5281 tcgcccagca agcctgctac ttgatggcgg ggcgcagcga ggccgggctc gtgcgcgccg
     5341 tccacgcgac ggatccgctg ctcgccgatc tcctgctgcg gcggctcgga gaagccactt
     5401 tgacgaggct actcgactga tggcgcgcac ggttctgctc atcgaaccgg acgtcgacgt
     5461 actcggcgcg ctcgcctcga agcttcgttc tcgggggctc gaggtgtgga tcgccgatgg
     5521 tctcgagagc gcggtcgcgc gggtgcgggc ggggcgtccg gacgcggttc tgctcgccat
     5581 cgagctcgga gtcgagaccg agcgcgcgct cgcggaagcg ctcggcacgc cgaccttcac
     5641 gtgctttcga ctggtcgatg aggcggagcc ggggcgcgca tcgctcttac cgcgcagtga
     5701 ccccgacgcg atcgcgcggc gcatgcacgc gctgccgacg gcttctgcca gcatcgtgcc
     5761 ggaggcgagc gacttccgtg gcgacctctc gcaggtctcg atcgtcgact tgattcagct
     5821 tttgggcgcg aaccgtcgca gcggagtgtt gctcctgcag acgccgctcg gcgcgggcga
     5881 agtacgcttc cgcgacggcg aagtcatcga tgcgctgtat cgccgcctgg agggaaaaaa
     5941 ggcacttttc aggctcctcg gggagcgcga aggtaccttc tcgttcgtcg gagggcacgc
     6001 cgggtcgtac atccatcgca tcgaggggcc gacgtatgcg ctgctgatgg acggcgttcg
     6061 ggagctcgac gaagcgcggg agctccgcgc ggcgctcgag ctcagcgaag acgctctcgt
     6121 cagcgtgcac ggggacgcgc agagcggcaa cgacgcgcag gcgctcgtgc tcggcatgtt
     6181 gatgtccccg cgtacgctcg ccgagctact cgacgatgtt ccggcgctcg acctcgacgt
     6241 gctgcaagca gtgaagcgcc tgctcgcagc gggaagtatc cgcaggatct ccggcggcac
     6301 ccggcgcatc gagctctccg accccgaccg gctgggagtg ctcgcggcgc ttgccaagcg
     6361 cgtggcgcgg ccgggatttc gcgacgccgg aaggctcgcc atcgcggcgg agccgggccg
     6421 attgctcggc atcgcggcct cgctgttgcg catcgccgag gcgcttccgc catccgaggc
     6481 agcgccgacg actcccatcc cgcacgtcct cgcaacgctt cggctcggtg agtcggtcga
     6541 tctcgacatc gtcgggatcc cactggtgca tgcctacgcg ccgctctggt cattggtgct
     6601 gccggggtgt tcggcggtgg ccgtgctcga c
//
LOCUS       DASTKS010001151         7638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1524166, whole genome shotgun sequence.
ACCESSION   DASTKS010001151 DASTKS010000000
VERSION     DASTKS010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524166"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..533
                     /locus_tag="VFQ35_24475"
     CDS             48..533
                     /locus_tag="VFQ35_24475"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9933892.1"
                     /translation="MSSKHISKNALERTSSAQSIAHPLNPRAKRKTLSLGDETGLRKC
                     AVHLNVVAPGDETTESHVHECVDEFIYIISGKGTVYLDDTAHEVSSGDFIGFPARGPA
                     HWMKNTGTTDLVYLVGGDRAEHDVCDYPHLEKRVYVTQVPDGRRLDFVDIKNVNSVRR
                     P"
     gene            complement(780..851)
                     /locus_tag="VFQ35_24480"
     tRNA            complement(780..851)
                     /locus_tag="VFQ35_24480"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Cys,seq:gca)
     gene            complement(999..1790)
                     /locus_tag="VFQ35_24485"
     CDS             complement(999..1790)
                     /locus_tag="VFQ35_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933893.1"
                     /translation="MRLPTVQAWAIVVLALPLARCATSQEIDPTVLGDHTGAGQSSTS
                     SGGATQASGGSSTSSGGAANNSGGAAQSEGGATFGSGGAPSSGGSSMASGGAIFGSGG
                     SMPASGGTSTASGGASIGSGGRIFGGSSGGTTSVAGAPGQGGTVSAQGGTTAKGGASS
                     SGGTTIGTGTGGSTSVAGATGSGGAAATGCLADWKSSTMCAPACTSRVQGDQLDCIKV
                     LDCYAANDCGPTTCAGNDQKCSSNKLGVSTAPYPYAQQVYDCLCK"
     gene            complement(2093..3031)
                     /locus_tag="VFQ35_24490"
     CDS             complement(2093..3031)
                     /locus_tag="VFQ35_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933894.1"
                     /translation="MTPVALAVVLLTLAASACTAKSPPAPSAQTPSSPPPTSARQPPT
                     QPGLPPEVAQLAQRTREHAVVLPTCAHVVENWPAEDLPQVALLGDAIVYDEKAVGSAR
                     AILASGKLQRVDELFEKLKARREAWKATHPHEEHPGTIAFLFDRKAPMAVVKSVFQTA
                     AFSGYPHLSFLVRKADAEERLARVLASAHVPAPLTALGNPSPKDPFRLGITQSQGQLS
                     IEWHYGTEGLNEPLPRLVSQPELGEQIRSEWERYGGHRSATDASFDQATLRVEDSSDF
                     ETMIRLLDALYTPLRDLRVGEYDVKVPAFDVTLEVN"
     gene            complement(3028..4041)
                     /locus_tag="VFQ35_24495"
     CDS             complement(3028..4041)
                     /locus_tag="VFQ35_24495"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9933895.1"
                     /translation="MLRTRRSCSEVDRRRSPAVIALTSALLSAFALNHSARADALPSN
                     SSPSSATTVAGPLVVELGPTPILVGQAVVASAPLALRGELARQLGPSRAILAKLRRGA
                     SAKDLRALLRAVRGTAADKLTLEVEAQSFETAIQEPSAAATSLVLELDEQHATLSRLK
                     KGELDASKEWVFGKAQSDAKANELLRKACKTSPCELRIVLGSTDSKVGAFEMLRALLA
                     ARARLPNLSLQFGETRFGTSAVTGRLPPELIQSLVRKSFAEFRHCYEAGLARDPNLEG
                     RISVRFVIGLDGSVTAARDAGSDIPDSRVRDCVIDAFLKLRFPEPDGGIVTVVYPIVF
                     QPE"
     gene            complement(4049..4600)
                     /locus_tag="VFQ35_24500"
     CDS             complement(4049..4600)
                     /locus_tag="VFQ35_24500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9933896.1"
                     /translation="MSEENPFKVLASREVYDNPWISVTEHRVEKPRGGEGIYGVVHFK
                     NRAVGVVPYQDGYVWMVGQYRFALGRYSWEIPEGGAPGSEAIEECARRELAEETGLSA
                     RRLDHLVTLHLSNSVTDEVAHIFIARDLSPGAASPEDTEVLRVKRMPLEEVHSLIQKG
                     EITDALTVTGILNVLLLRAQGLL"
     gene            complement(4634..7045)
                     /locus_tag="VFQ35_24505"
     CDS             complement(4634..7045)
                     /locus_tag="VFQ35_24505"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933897.1"
                     /translation="MKYGYFDDANREYVITTPKTPVKWVNYVGTMRFGGIVDHTGGTL
                     LCKDDPALNRIVKYIPQLPSSDFKGETLYLRFKENGRYRVFSPYFVPTLDPYDRYECH
                     VGLGYNRYVSEFYGIRTEVTIFVPRGATLSVRRIRVTNLRSEALELDAIPVVEYTHFD
                     ALKQFNNADWVPQTMQSRGIKNAKGELLLLQYAFMNKDRQVNYFTSSMPVSSFESDRK
                     RFLGDNEYGTFKQPLSLLNDELGNYEALRGDNIGALLHHLGSIPAGETRELIVLLGQA
                     PSAEAAEKDAEKYRSSKAVEAELEAQRAFWDDYLSKLQAKTPDAAFNSMVNVHNPRQC
                     YTTKNWSRYLSLYQLGLGERGIGFRDSSQDVLGVLDRVPSEARDLMVMLLSVQKRNGS
                     AMHQLNPLTMHANEGDSREREDRPKYYGDDHLWIVLSVCWYLKETGDLDFLQKSVPFY
                     DKDKQGNTVESGSVLEHLKRSVAFTKGDLGAHKLPLLGFADWNDTVNLATGAESMFNA
                     NLYGVALQELIQLCEKLGDKAAADGYRADYDTMKQAFNACAWDGEWFVRYFDHDGSPI
                     GSSKNSEGQIYTNGQSWPVISGFATPEHAKKALESVNRLLNTKNGIKLSWPGYNGFDQ
                     NKGGVTTYPPGAKENGGIFLHANPWVMIAETRLGNGDRAYQYYNQINPAARNDSIDTF
                     ECEPYAYPQNILGDEHPQFGLARNSWLSGTSAWTYTAATKFILGIQPTYDGLQVEPCI
                     PTAWGEYSVTRSFRGATYEITVKNPKHVSKGVSEMRVDGRVVPGNVVPLADSGSKVRV
                     EVTLG"
     gene            complement(7329..>7638)
                     /locus_tag="VFQ35_24510"
     CDS             complement(7329..>7638)
                     /locus_tag="VFQ35_24510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933898.1"
                     /translation="VIGTDALARFSEGVGLLIHDSQYVLSDMPHKLGWGHSIVDDVLK
                     LGALAQPQRLALFHHDPDRTDEALDELGARSRAWMTQNAPGVEVVVAGEGLAFTLAGR
                     "
BASE COUNT         1272 a         2400 c         2494 g         1472 t
ORIGIN      
        1 agccggaact ttgcgctccc cgaactccgt cttagggtct tctcgtcatg tcttcgaagc
       61 acatctcgaa aaatgccctc gagcgcacgt cttcggccca gagcatcgct cacccgctga
      121 acccacgggc caagcgcaag accctctcgc tcggcgacga aaccggtctc cgcaaatgcg
      181 cagtgcacct caacgtcgtc gcgcccggag acgagaccac cgagtcgcac gtccatgagt
      241 gcgtcgacga attcatctac atcatctcgg gtaagggcac cgtctacttg gacgacactg
      301 cacacgaagt ctccagcggc gacttcatcg gctttcccgc gcgcggcccg gcgcattgga
      361 tgaaaaacac cggtaccacc gatctcgtct acctggtcgg cggtgaccgc gccgaacacg
      421 acgtttgtga ttacccgcac ctcgaaaagc gcgtctacgt aacgcaagtt cccgacgggc
      481 gccgcctcga cttcgtcgac atcaagaacg tgaacagcgt tcgaaggccg tgagctttct
      541 caagttcgcc cttcgtggac gactacgcga acttccgctc gtacgcgctc ggcgaaggct
      601 cggcgaacgc tcggaacatc tcgcgttgtc gcttcgaact gcaccgagaa cgacaaacca
      661 gaccgaaccg agacgcggtt ccccagggcg cagactcgag tttggcgcct acgagccgta
      721 cgcggggacg gtgagggcta gcggaaccgg aatttgcgtc gggagccgag agagctttga
      781 ggtgcggatg ggattcgaac ccacgtatga tggttttgca aaccattgcc tagccgcttg
      841 gctaccgcac cggatgtttc tgaagcgttt gctcaacgag cgccgggaag ctgccacggt
      901 aagctagtta gatcaacccc ctgcgcgtcc ctcgcgtccg cgctcatccc agaatgttct
      961 gggttaccgg cctaggggcc cgcggtcagc gcgccgagct acttgcagag gcagtcgtag
     1021 acctgttgcg cgtacgggta cggagcggtg ctgaccccga gcttgttcga gctgcatttc
     1081 tggtcgttcc cggcgcacgt ggttggcccg caatcgttcg ccgcgtagca gtcgagcact
     1141 ttgatgcagt ccagttggtc gccttgaact cggctggtgc aggcaggcgc gcacatggtg
     1201 ctcgacttcc aatcggccaa acagcccgtg gctgcggcgc cgccggagcc ggttgcacca
     1261 gcgacgctgg tgctgccgcc cgtgcccgta ccgatggttg tgccgccgct agatgacgct
     1321 ccacctttcg cggtcgtgcc gccctgggcg ctgacggtgc ctccctgccc gggagctccc
     1381 gcgacgctcg tggttccgcc gctgctgcct ccgaagatcc tgccgccgct gccgatgctg
     1441 gctccgccac ttgcggtgct ggtgcctccg ctcgcgggca tgctgccgcc gcttccgaaa
     1501 atggcgcctc cgctcgccat ggagctgcca ccgctgctcg gcgcgcctcc gctcccgaag
     1561 gtcgcgccgc cttcgctctg cgcggcgcca ccactgttgt tggcagcccc accgctgcta
     1621 gtgctcgagc ctccgctggc ttgagtcgcg ccgccgctcg aagtggagct ttgcccggca
     1681 cccgtgtggt cgcccagtac ggtcggatcg atctcctgac tggtcgcaca gcgagcgagc
     1741 gggagtgcaa gtacgacgat ggcccaggct tggacagtcg gaaggcgcac tgagaaactc
     1801 ctagagcaaa gaagcacgcg agccgcgccg cgcgtaggtc gttcgcagct cgtgctggtg
     1861 tcagtctgaa tcagtgttgc acgcagcgcg aactttcgcc atgggtcgcg agagactacg
     1921 cattgaaccc tcgcgcttca tgcgcagagt cgatgtcgtt cgtgagtcaa tcaacctgac
     1981 gagtgattag gcgcagtcgg tgcgctcatg tgcgactctg ccggacgagg tagcgaggca
     2041 aacgccttca tccgacgctg aacgcagatc ggattcgaag agccgattgc gctcagttga
     2101 cttcgagcgt gacgtcgaag gcggggacct tcacgtcgta ttcgcctacg cgcaagtcac
     2161 gcaacggcgt gtacagcgcg tcgagcaggc gaatcatcgt ctcgaagtca ctcgagtcct
     2221 ccacgcgcag ggttgcctgg tcgaaggagg cgtcggtcgc gcttcggtgt ccaccgtaac
     2281 gttcccattc actgcgaatt tgctcgccga gctcgggttg cgacacgagg cgtggcagcg
     2341 gctcgttcag gccttcggtg ccgtagtgcc actcgatgct cagctgccct tggctctgcg
     2401 taatgcccaa tcgaaatggg tctttcgggc tcgggttgcc caaggcggtc aggggcgccg
     2461 gcacgtgcgc gctggcgagc actcgggcaa ggcgttcttc ggcgtcagcc ttacgaacca
     2521 agaagctcag gtggggataa ccagagaacg ccgcggtttg gaacacactc ttgaccacgg
     2581 ccataggggc ctttcgatcg aacaaaaacg cgatcgtgcc cgggtgttct tcgtgcgggt
     2641 gggtcgcctt ccaagcttct cgtcgtgcct tcagcttctc gaacaactcg tcgacgcgct
     2701 gcagcttgcc gctcgcgagg atggctcgcg cgctcccgac cgctttttcg tcgtacacga
     2761 tcgcgtcgcc gagcaaggcg acctggggca agtcttctgc cggccagttt tcgacgacgt
     2821 gcgcgcaggt tggaaggact acggcatgct cccgtgtgcg ctgtgcgagt tgtgcgactt
     2881 cgggcgggag cccaggctgc gtaggcggtt gccgggcgct ggtcgggggc ggcgaggacg
     2941 gcgtctgtgc gcttggcgca gggggcgatt tcgcggtgca ggccgatgcc gcgagcgtga
     3001 gcaggacgac ggcgagggcg acgggtgtca ttctggctga aacacgatcg gataaacgac
     3061 ggttacgatt ccgccgtcgg gttcaggaaa gcgaagcttc aggaacgcgt cgatgacgca
     3121 gtctcgcacc ctcgagtcgg gaatgtccga gcccgcgtct cgagcggcgg tcacgctgcc
     3181 atcgaggccg atgacgaagc gcacggagat cctcccttcg aggttcggat ctcttgcgag
     3241 gccggcttcg tagcagtgcc gaaactccgc gaagctcttg cggacgaggc tttgaatcag
     3301 ctccggcggc agccggcccg tgactgcgct ggtgccgaac ctggtttccc cgaactggag
     3361 cgacaggttg gggagtcgcg cgcgagcggc gagtagggcg cgcaacatct cgaaagcgcc
     3421 caccttcgaa tcagtcgagc cgagcacgat ccgaagttcg catggcgagg tcttgcatgc
     3481 cttgcgcagc aactcatttg ccttcgcgtc ggattgcgcc ttgccgaaca cccactcctt
     3541 gctcgcgtcc aattcgccct tcttcagccg ggagagggtt gcgtgttgct cgtcgagctc
     3601 gagtacgagg ctggtcgcgg ccgcactagg ctcttgaatc gccgtttcga agctctgggc
     3661 ttccacctcg agcgtcagct tgtcggctgc agtaccgcga acggcgcgga gaagcgccct
     3721 gaggtctttc gccgaggcgc ctcgacggag cttcgcgagg atcgcgcgcg atggcccgag
     3781 ctgccgcgcg agttcgccac gcaaggcgag gggagcgctg gcgaccacag cctggccgac
     3841 caagattgga gtgggtccga gctcgacgac gagcgggcca gctacggtcg tggcggagct
     3901 cgggctcgaa ttcgacggaa gcgcgtcggc acgcgcgctg tgattgagcg cgaacgccga
     3961 aagtagagcc gaagtcagcg caatgaccgc aggactgcgg cgccggtcga cctcagagca
     4021 actcctacga gtgcggagca tatcgacctc agagcaatcc ttgggctcgc aggagtagga
     4081 cattgaggat gcccgtcacc gtgagcgcat ccgtgatctc gcctttttga atcagcgagt
     4141 ggacctcctc gagcggcatg cgcttgaccc tgagcacttc ggtgtcttcc ggagacgcgg
     4201 cgccggggct gaggtcgcgc gcgatgaaga tgtgcgcgac ttcgtcggtc accgagttcg
     4261 agagatgtag cgtgacgagg tgatcgaggc ggcgagcgct gaggccagtt tcctcggcga
     4321 gttcgcgccg cgcgcactcc tcgatggcct cgcttcctgg cgcgccgccc tcggggattt
     4381 cccatgaata gcggccgagc gcgaaccggt actgccccac catccacacg tagccgtctt
     4441 gatacggcac gacacctacg gcgcggttct tgaagtgaac gacgccatag atgccttcac
     4501 cgcctcgcgg tttctccacg cggtgctcgg tcacggagat ccacgggttg tcgtagacct
     4561 cgcgcgacgc gagcaccttg aaggggttct cttcactcat ggagaccgcg cgcctttgcg
     4621 tacggcgctc gggtcagccg agcgttacct cgacgcggac cttgctgccc gagtccgcga
     4681 gcggcacgac gttgccaggt acgacccgcc cgtcgacgcg catttcgctc acgcccttcg
     4741 agacgtgctt cgggttcttc acggtgatct cgtaggttgc gccccggaag ctgcgcgtga
     4801 cgctgtactc accccacgcc gtcggaatgc agggctcgac ctgcagaccg tcgtacgtcg
     4861 gctgaatgcc gaggatgaac ttggtggcgg ccgtgtaagt ccacgccgac gttccggaaa
     4921 gccagctgtt acgcgcgagg ccgaactgcg ggtgctcgtc gccgaggatg ttctgcgggt
     4981 acgcgtaggg ctcgcactcg aaggtgtcga tgctgtcgtt gcgcgcggcc gggttgatct
     5041 ggttgtagta ctggtaggcg cggtcgccgt ttccgagccg cgtctccgcg atcatgaccc
     5101 aggggttcgc gtgcaagaag atgccgccgt tctccttggc gcccggcggg taggtggtca
     5161 cgccaccctt gttctggtcg aagccgttgt aaccgggcca gctcagcttg atgccgttct
     5221 tggtgttgag caggcggttc acgctctcga gcgccttctt cgcgtgttcg ggagtcgcga
     5281 agccgctgat cacgggccac gactggccat tcgtgtaaat ctggccttcg ctgttcttgc
     5341 tgctgccgat cggcgagccg tcgtgatcga agtagcgaac gaaccactcg ccgtcccaag
     5401 cgcaggcgtt gaacgcttgc ttcatggtgt cgtagtcggc gcggtagccg tcggcggccg
     5461 ccttgtcacc gagcttctcg cagagctgaa tgagctcttg cagcgcgacg ccgtacaggt
     5521 tcgcgttgaa catcgactcg gcgcccgtcg ccaggttcac ggtgtcgttc cagtctgcga
     5581 acccgagcag cggtagcttg tgcgcgccga gatcgccctt ggtgaatgcg accgagcgct
     5641 tcaagtgctc gagcacgctg ccggactcga cggtgttgcc ctgcttgtcc ttgtcgtaga
     5701 agggcacgct cttctgcaag aagtcgaggt cgccggtctc tttcaggtac cagcagaccg
     5761 acagcacgat ccagaggtgg tcgtcgccgt agtacttcgg gcggtcttcg cgctcgcggg
     5821 agtcgccctc gttcgcgtgc atcgtgagcg ggttcagctg atgcatcgcg gaaccgttgc
     5881 gcttctgcac gctgagcagc atcaccatga ggtcacgcgc ttcgctcggc actcggtcga
     5941 gcacgcccag cacgtcttgc gagctgtcgc ggaaaccaat gccgcgctcg ccgaggccaa
     6001 gctggtagag cgacaggtag cgcgaccagt tcttggtcgt gtagcactga cgcgggttgt
     6061 ggacgttcac catcgagttg aaggcggcgt ccggcgtctt cgcttggagc ttcgagaggt
     6121 agtcgtccca gaaggcgcgc tgagcctcga gctcggcttc gaccgccttc gacgagcggt
     6181 acttttccgc gtctttttcg gctgcttcgg cgctgggcgc ctgcccgagc aacacgatga
     6241 gctcgcgcgt ctcgcccgcc gggatcgagc cgaggtgatg caggagcgcg ccgatgttgt
     6301 caccgcgtag cgcttcgtag ttgccgagct cgtcgttgag cagcgagagc ggctgcttga
     6361 acgtgccgta ttcgttgtcg ccgaggaaac gcttgcggtc cgactcgaaa gacgaaaccg
     6421 gcatgctgga ggtgaagtag ttgacctggc ggtctttgtt catgaacgcg tactggagca
     6481 gcaggagctc gcctttcgcg ttcttgatgc cgcggctctg catcgtctgc ggaacccagt
     6541 cggcgttgtt gaactgcttc agcgcatcga aatgcgtgta ctcgacgacg gggatcgcat
     6601 cgagctcaag cgcttcgctg cgcagattgg tgacgcggat gcggcgcacc gagagcgtcg
     6661 cgccgcgcgg cacgaagatc gtcacctcgg tgcggatgcc gtagaactcg ctgacgtaac
     6721 ggttgtagcc gaggccgacg tggcactcgt aacggtcgta cggatcgagc gtcggcacga
     6781 agtacggaga gaacacccgg taccgcccgt tctctttgaa gcgcaggtac agcgtctccc
     6841 ccttgaagtc actggacggt agctgcggga tgtacttgac gatgcggttg agcgccgggt
     6901 cgtccttgca caagagcgtt ccgccggtgt gatcgacgat gccgccgaag cgcatggtcc
     6961 ccacgtagtt cacccacttg accggagtct tcggggtcgt gatcacgtat tcgcggtttg
     7021 cgtcgtcgaa atagccgtac ttcatgggtc cttgtggggt gagctggttt tcgggaaggg
     7081 gcggcgaagg ttagcgctgt cgctcgcggc ggaaagcccc gaatcaccaa aaaattccag
     7141 tttggcggcg ctcgcctgat ccgctccgtg gtgccgcgac ggcaacccgg gcgaaatcgg
     7201 caaaaactgc gcgccgcacg ctactggaat cggttccaga aaagccccgc ctttccctgc
     7261 cgcgctgcgg caggagcgag aaacctgcta tcggcgccgc gagggctgcg cgaatccgcg
     7321 cccgggcctc aacgacccgc gagggtgaag gcgagccctt cgccggccac gaccacttcg
     7381 accccgggag cgttttgggt catccaagct cgcgagcgcg cgccgagctc gtcgagcgcc
     7441 tcgtcggtgc gatctggatc gtggtggaac aacgcgagcc gctgcggttg tgcgagcgcc
     7501 ccgagcttga gcacgtcgtc cacgatggag tgcccccaac cgagcttgtg cggcatgtcg
     7561 ctcagtacgt actgggagtc gtggatcaag agaccgacac cttccgagaa gcgcgcgagc
     7621 gcgtccgtcc caatgacg
//
LOCUS       DASTKS010001152         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1524248, whole genome shotgun sequence.
ACCESSION   DASTKS010001152 DASTKS010000000
VERSION     DASTKS010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524248"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFQ35_24515"
     CDS             <1..705
                     /locus_tag="VFQ35_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9933899.1"
                     /translation="DDGKLEGGRAVEPRVYLEQFGFESGQLRQPVGSLSGGERARVAL
                     ARLLRRVMNLLLLDEPTNDLDVATLGSLESLLLESNASALVVTHDRWFLDRVASAILC
                     FEGDGKVVLYPGNYDTFRRLRAERDSTRTVVAEASVQESPKKKATTPPKARRLNRAEE
                     QELANLPDAIDRGEAEVARCQAELSDPATQAGGRDTVQRATAALAAAEAEVARLMARW
                     EELEQKRAELAAGGAS"
     gene            702..1283
                     /locus_tag="VFQ35_24520"
     CDS             702..1283
                     /locus_tag="VFQ35_24520"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9933900.1"
                     /translation="MSSRPNALDGRTVGIIVRMVVGLAALLVGVAVLSALFRSELEAL
                     GHVFVDRFGLWGMVIGSLVADGFHFLLPPQFYMLMGISSGVPARATLTAVCFGSFVGG
                     WTAYFLATRIGRFEVLERRLAEPRRLLESALDRYGNFALVLVGFLPITYAALCYLAGL
                     GRVSKRGFLIIALVRVPRLVAYYYLVRLGWLGV"
     gene            complement(1250..3235)
                     /locus_tag="VFQ35_24525"
     CDS             complement(1250..3235)
                     /locus_tag="VFQ35_24525"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9933901.1"
                     /translation="MLTSSARRARATSWAFACAVLLHAAFGCHPPYANSQPAPGPTNT
                     TALASSAPAPAPSAVAEWGEADAPVELVEYGDLECPFTGRAAPVVAQIQEVYGPQRVH
                     LVWRHFPLPFHAAAPKAHEAAEAVRTLGGNSAFFRFTALALAHRETLDDDSFATWAEQ
                     SGVPRARFTSAMQQGVGRAALERDLQLGKAQGVTGTPEFFVNGVLISGAQPFEAFRDA
                     IDRELGETQKLLASGVPRAELAARRTQQNFKKPEPKPERESSDEDGTVWMVPVAPSDP
                     VRGPSDALVTVVLWSDYQCPFCKRVEPTLAELRALYKDDVRIVWKDNPLPFHPRALPA
                     ALLARTAYATRGNDVFWQVHDALFDAQEDLSDEALGEIARKFAVRFAPEDKSALRQRI
                     RAEVDESASLAADLKANGTPHFFVNGVRLVGAQPLEKFRELIDAELAKARALKSSGVA
                     SKDVYAEVMKGATPPPPPQTKEVPAPDATTPVRGNARARVTMQVFSDFQCPFCRRVNP
                     TLAELEREFGKDLRIAFRHEPLPFHEHAELAAEAAQEAFAQKGSAGFFAYHDRLFAAQ
                     DDAGGLERENLESIAKEVGLDLTRFRAALDSHRHADKVRADLEIASKAKISGTPGFVI
                     NGYFLSGAQPAAAFRRLIRRALGEATRQTPSQPSRTK"
BASE COUNT          479 a         1038 c         1181 g          576 t
ORIGIN      
        1 gacgacggca agttggaggg tggtcgtgca gtcgagccgc gcgtctacct cgagcagttc
       61 ggcttcgaat cgggtcagct gcggcagcca gtgggctcgc tctccggcgg cgaacgggcg
      121 cgcgtggcgc tcgcgcgctt gctgcgccgc gtgatgaact tgctgttgct cgacgagccc
      181 acgaacgatc tcgatgtggc gacgctgggc tcgctcgagt cgctgctgct cgagtcgaac
      241 gcgagcgcgc tcgtcgtcac gcacgatcgc tggttcctcg accgcgtcgc gagcgccatc
      301 ttgtgcttcg aaggcgacgg caaggtggtg ctctatcccg gcaactacga cacgttccgc
      361 cggctccggg cggagcgcga ctccacgcgg accgtggtgg ccgaggcgag cgtccaagag
      421 tccccgaaga agaaggcgac cacgccgccg aaagcgcggc gcttgaaccg cgccgaggag
      481 caagagctcg cgaacctgcc cgatgcgatc gatcgcggtg aagccgaggt cgcgcgctgc
      541 caggcggagc tctcggatcc ggcgacgcaa gcgggcgggc gcgatacggt tcagcgcgcg
      601 acggctgcgc tcgcggctgc cgaagccgag gtcgcacggc tgatggcgcg ctgggaggag
      661 ctcgagcaaa agcgcgcgga gctcgcggcg ggaggcgcga gttgagttcg cgcccgaacg
      721 cgctcgacgg gcgcaccgtc gggatcatcg tgcgaatggt ggtcgggctc gcggcgctgc
      781 tcgtgggtgt tgccgtgctg agcgcgctgt ttcgctccga gctcgaggcg ttgggacacg
      841 ttttcgtgga ccgcttcggt ctctggggga tggtgatcgg gtcgctcgtg gcagacggct
      901 tccacttcct gctgccccct cagttttaca tgctgatggg catcagctcg ggcgttcccg
      961 cgcgcgcgac gctcaccgcg gtctgcttcg gttctttcgt cggcggctgg acggcgtact
     1021 tcttggcgac acgcattggg cgcttcgagg tgctggagcg ccggttggcc gaaccgcgtc
     1081 ggttgctcga gagcgcgctc gaccgatacg gcaacttcgc cctcgtgctc gtgggatttc
     1141 tgccgatcac ttacgccgcg ctctgctacc tcgccgggct cgggcgcgtc tcgaagcgcg
     1201 gttttttgat cattgcgctc gttcgggtcc cgcggctcgt cgcgtattac tacttggttc
     1261 ggctcggctg gctcggcgtc tgacgcgtcg cctccccgag cgcgcgccga atcaagcggc
     1321 ggaacgcggc cgcgggctgc gcgccgctca agaaatagcc gttgatgacg aagcccggcg
     1381 tgccggagat cttcgctttc gaagcgattt cgagatcggc gcgcaccttg tcggcgtggc
     1441 gatgcgaatc gagcgcggcg cggaagcgcg tcagatcgag gccgacctct ttcgcgattg
     1501 actcgaggtt ttcgcgctcg agaccgcccg cgtcgtcttg cgcggcaaag agccgatcat
     1561 ggtaggcgaa gaagccggcg ctgcccttct gggcgaacgc ctcttgggcg gcctcggccg
     1621 cgagctcggc gtgctcgtgg aacggcaggg gctcgtgacg aaacgcgatg cgcagatctt
     1681 tgccgaactc gcgctcgagc tcggcgagcg tcgggttcac gcgccggcaa aaggggcact
     1741 gaaaatccga gaacacctgc atcgtgacgc gcgctcgcgc gttgccgcgc accggggtcg
     1801 tggcgtccgg cgcgggcacc tctttggttt ggggtggggg aggcggcgtc gcgcccttca
     1861 tgacctcggc atagacgtct ttgctcgcga ccccgctgct cttgagcgcg cgggccttcg
     1921 cgagctcggc gtcgatgagc tcacggaact tttcgagcgg ctgagcgccg acgagccgca
     1981 cgccgttgac gaagaagtga ggcgtgccgt tggctttcag atccgcggcg agggaggcgc
     2041 tttcgtcgac ttcggcgcgg attcgctgac ggagcgcgct cttgtcttcc ggcgcgaagc
     2101 gtaccgcgaa tttgcgcgcg atttcgccga gcgcttcgtc gctcaaatct tcttgggcgt
     2161 cgaagagcgc gtcgtggact tgccaaaaca cgtcgttgcc gcgcgtcgcg tatgccgtgc
     2221 gtgcgagcaa cgcggcaggg agggcgcgcg gatggaaggg cagcgggttg tctttccaga
     2281 cgatacgcac gtcgtccttg tagagagcgc ggagctcggc gagcgttggc tccacacgct
     2341 tgcagaacgg gcactggtag tcggaccaga gcacgaccgt gacgagcgcg tcactcggcc
     2401 cccgcacggg atcgctcggc gcgaccggaa ccatccaaac cgtgccgtcc tcatccgagc
     2461 tctcccgctc gggcttgggc tcgggcttct tgaagttttg ctgggtgcgg cgagccgcga
     2521 gttccgcgcg cggtaccccg ctcgcgagga gcttttgcgt ttcgccgagc tcgcgatcga
     2581 tcgcgtcgcg aaaagcctcg aacggctgtg cccccgagat caggacgccg tttacgaaga
     2641 actcgggagt gccggtgacg ccttgcgctt tgccgagctg aaggtcacgt tcgagcgcgg
     2701 cccgcccgac gccctgctgc atcgcgctgg tgaagcgagc gcgtggcaca ccggactgct
     2761 ccgcccaggt ggcgaacgag tcgtcgtcga gggtttcacg atgcgcgagg gcgagcgccg
     2821 tgaaccgaaa aaacgcagag ttcccgccga gcgtgcgcac cgcttcggcc gcctcgtgag
     2881 ccttcggcgc cgcagcgtgg aacggtagtg ggaagtgccg ccaaacgagg tgcactcgct
     2941 gcgggccgta cacttcttgg atttgcgcga cgacaggggc ggcgcgcccg gtgaacggac
     3001 actcgagatc gccgtactcg acgagctcga ccggcgcatc ggcttcgccc cattccgcca
     3061 ccgcgctcgg cgccggcgcg ggcgcgctcg aggccagagc cgtggtgttg gtggggcccg
     3121 gcgctggttg gctattcgcg tagggaggat gacaaccgaa ggcggcgtgg aggaggacgg
     3181 cacaagcgaa agcccaactg gtggctcgcg ctcggcgagc ggatgatgtc agcatggtgg
     3241 aagttgcgat tcagcggcgc cgagaccgaa cctc
//
LOCUS       DASTKS010001153         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_629243, whole genome shotgun sequence.
ACCESSION   DASTKS010001153 DASTKS010000000
VERSION     DASTKS010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629243"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFQ35_24530"
     CDS             <1..306
                     /locus_tag="VFQ35_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933902.1"
                     /translation="RFSAPFALLAACALLLPLTRKRASLYRDPIALWRDAAERTNYSV
                     RPLVNLGTLLARDGQLREARATLSLALLRDPTRYDTRLRLDAVRRASLAPAPAHAAP"
     gene            319..1155
                     /locus_tag="VFQ35_24535"
     CDS             319..1155
                     /locus_tag="VFQ35_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933903.1"
                     /translation="MTFPNTRRRRLYAVIGLFAAALIPLGVALATPMQCIKDGQMCNK
                     DLLCAFKVELAEKILLYETFLGNSPATKKSKGSTRQGVRYSGALYDAALAEAKAENPG
                     ASAAELAAAAYQKFTTKVRAKLGEEASKYKDCTALGVTPNDTYRGTWSGMHTDKNDCN
                     VYGDIGTGDSHESVALDDLKRRTEGCLEVYQSDRGHEAVHQDFCMRRLRKELPASDGL
                     QGYIDEDIEAYRYSLQHAANDLTRLQIQCTTDPSTDQFRKRADDLLKKLKQYKINQEG
                     QP"
     gene            1152..>2455
                     /locus_tag="VFQ35_24540"
     CDS             1152..>2455
                     /locus_tag="VFQ35_24540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933904.1"
                     /translation="MNERSALRRIASRTVLAWLASAALVIAAGPASADVIRLDSDTAS
                     PSFDPAADFDTQCLGPTPEADCDARAALIEAELFQLLAQLENDEAPKTLAIFQGAIDL
                     DSPLIQAIAMRYLARAEQQPTDFMSKVKMFLLGPDAPLGVSAAEVLRDSALEPDQKLS
                     ELYRQQRTAADYALASTPAADADPASEDLVQACTKDARLEQMPSFAADEQFSPAARLL
                     MYDRFLPATFEVGKDYPVTSFVTDASVAEVSAFFTKRFGQSYGPYADAQTRYQSLSLE
                     LVSLQAAAQKGDQAAIARIQQIGEELVSLQPIITLGGFLQLPAIHAENDLVWVDGNPD
                     DLTTSPSRVVTVGADALLEKTVIRYINAPKSGGSGGAGGAGGSNGAGGRASGGRASGG
                     AGGLAGAAGENSGKSDSGCGCAIPGAPRGRTGLLALLMAAVFG"
BASE COUNT          493 a          848 c          745 g          369 t
ORIGIN      
        1 cgcttcagcg ccccgttcgc gttgctggcc gcctgcgcgc tgctgctccc gctcactcga
       61 aagcgcgcgt cgctctaccg tgaccccatc gcgctgtggc gcgacgccgc cgagcgaacc
      121 aattacagcg ttcggccgtt ggtgaacctc ggcacgctgc tcgcacgcga cggacagctc
      181 cgcgaagcgc gcgcgacgct ctctctcgca ctgctgcgcg atccgacccg ctacgacact
      241 cgcctgcgcc tcgacgccgt gcgccgcgcc tcactcgccc ctgccccagc gcacgccgct
      301 ccatagaaag caacgaacat gacgtttcca aacacccgac gaaggcggct ctacgccgtc
      361 attggcctct tcgccgccgc gctaatcccg ctcggcgttg ccctcgcgac gccgatgcaa
      421 tgcatcaagg atgggcagat gtgcaacaaa gatctgctct gcgcgttcaa ggtggagctc
      481 gccgagaaga tcctcttgta cgagacattc cttgggaact ctccggcgac gaagaagtcg
      541 aaggggagca cgcgccaggg cgttcgatac agcggagcgc tctacgacgc cgcgctcgcc
      601 gaagcgaaag ccgaaaaccc gggcgcgagc gcggccgaac tcgcggctgc ggcttaccaa
      661 aaattcacga ccaaggtgcg cgcgaagctc ggggaagagg cttcgaagta caaagattgt
      721 acggcgctcg gagtgacgcc gaacgacacc taccgaggga cgtggagcgg aatgcacacg
      781 gacaagaacg actgcaatgt ctacggcgac atcggcaccg gggacagcca cgagtcggtg
      841 gcgctcgacg acctcaagag gcgcaccgag ggctgcctcg aggtttacca gagcgatcgc
      901 ggacacgaag ccgtgcatca agatttctgc atgcgcaggc tccgcaaaga gctgcccgcg
      961 tccgacgggc ttcagggcta catcgacgaa gacatcgaag cgtatcgata ctctctccaa
     1021 cacgcggcca acgaccttac gcgccttcaa attcagtgca ccaccgatcc cagcaccgac
     1081 caattcagaa agcgcgccga tgacctcttg aagaagctca agcagtacaa gatcaaccag
     1141 gaggggcagc catgaacgaa cgctccgcgc tgcggcgcat cgcctcacgc accgtcctcg
     1201 cgtggctcgc ttctgccgcg ttggtcatcg cggcggggcc ggcgagcgca gatgtcattc
     1261 ggctcgactc agacaccgca agccctagct ttgacccagc ggcagacttc gacacccagt
     1321 gcctgggccc aaccccagaa gcggactgcg acgcgcgtgc cgccctcatc gaggctgagc
     1381 tcttccagct cctcgcccag ctcgagaacg acgaagcgcc gaaaaccctg gctatcttcc
     1441 aaggcgccat cgacctcgac tcgcctctca ttcaggcgat cgcgatgcgc tatttggccc
     1501 gcgccgaaca acaaccgacc gacttcatgt ccaaggtcaa gatgttcctg ctcgggcccg
     1561 acgcgccgct cggtgtctcc gcagccgaag tactgcgtga ctcggcacta gagccggacc
     1621 agaagctgtc cgagctctat cgccagcaac gcacggccgc ggactacgcg ctcgcgtcga
     1681 cgcccgccgc cgacgcagat cccgcgagtg aagacctggt tcaggcgtgc acgaaagacg
     1741 caagactcga gcagatgcct tcattcgccg ccgacgaaca gttctcgcct gccgctcgcc
     1801 tgctcatgta cgaccgtttc ctgccggcga cgttcgaagt cggcaaagac tacccagtca
     1861 cgagcttcgt tacggacgcg agtgtggcgg aggtttccgc attcttcacg aagcgcttcg
     1921 gacagtcgta tggcccgtac gctgacgccc aaacacgtta ccagtcgctc agcctcgagc
     1981 tcgtgagcct ccaagccgcg gcgcaaaagg gcgaccaagc agcgatcgcg agaattcagc
     2041 agattggcga ggagctcgtc tcgctccagc cgatcatcac gctgggcggc tttctccagc
     2101 tgccggccat tcacgccgag aacgatctcg tttgggtcga cggaaaccca gacgacctca
     2161 cgacgagccc gtcgcgcgtg gtgacggtcg gcgccgacgc gctactcgaa aagaccgtga
     2221 tccgctacat caacgcgcca aagagcggcg gaagcggggg cgccggcgga gcgggcggca
     2281 gcaatggcgc tggcggccga gcaagcggcg gccgagcaag cggcggagcg ggcgggctcg
     2341 ccggcgccgc gggagagaac agcggcaagt ccgatagcgg ttgtggctgc gcgatacccg
     2401 gcgcaccgag aggacgcacg ggacttctcg ccctgctcat ggcggccgtg ttcgg
//
LOCUS       DASTKS010001154         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1301404, whole genome shotgun sequence.
ACCESSION   DASTKS010001154 DASTKS010000000
VERSION     DASTKS010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301404"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFQ35_24545"
     CDS             <1..374
                     /locus_tag="VFQ35_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933905.1"
                     /translation="ELVAARHPHVELCVTHELTPELSELVARAELAVFVDARHDRLDE
                     GVLSEALKPATTSPLSGHYSNPRSLLALSQALYGRYAPAWLVTLPAHDTGLGEGISEC
                     ARRTVPAALRRVDELISQALP"
     gene            371..2626
                     /gene="hypF"
                     /locus_tag="VFQ35_24550"
     CDS             371..2626
                     /gene="hypF"
                     /locus_tag="VFQ35_24550"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET9933906.1"
                     /translation="MTRWRAEFHGIVQGVGFRPRAYREARERALSGWVQNRGGRVELE
                     VEGPSAAIEDFIETLRRSPAPARIEHIERMELAETHETGFHIVTSQSAEVEAALPADL
                     APCAECERELRDPANRRYRHPFTSCVACGPRYSIARGLPYDRELTTMASFPLCPTCRR
                     EYEDPSDRRYHAEPIACPECGPQLTLVTAGGTELATGTSALYEAVRRLREGEIIALRG
                     LGGYQLLVDARSSSSVRRLRERKHREEKPLAVLFASLAEVRSACDISAGEAELLASPA
                     RPIVLVKRRSGAPIAPDVAPGLSELGVFLPPTPLLSLLLAALDFPIVCTSGNLSGEPL
                     CIDNTEALVRLASVADAFLIHDRVIARPLDDSVVRLGARGLQSLRRARGYAPLPVARF
                     ETGEAVVALGGYQKSTIALAWNGRAILSQHLGDHDNAKSLELFERTLDDLLAFYGVRP
                     EVIACDQHPDYPSSAWGEALSRRLGARLLRVQHHHAHAAAVLAEHGVDQRALALSWDG
                     TGLGSDGTIWGGEALVVQGARFQRLGHLGTFPLLGGDQAAREPRRCALGLLFATAPER
                     ARDFAHEHFRGEAAQVLLAALERRVNAPLTSSLGRLFDAVASLLGIRQRCSYEGQAAM
                     ELEALARREHAAGGYALPFSAGALFVADLGALVEQVTADLARRESPARIAARFQNALV
                     GYGVALAARAGLECVVLGGGCFQNQYLRDELARRLTELGHRVLTADTVPTNDGGLALG
                     QAWVASQQSRD"
     gene            2638..2877
                     /locus_tag="VFQ35_24555"
     CDS             2638..2877
                     /locus_tag="VFQ35_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9933907.1"
                     /translation="MCLGIPGRLVKRTRAEPPFGSVAFAGVEREICLAYTPEAEPGDY
                     LIVHAGFAISIVDAAEAEATLDALRELEVTEPVPP"
     gene            2874..>3567
                     /gene="hypD"
                     /locus_tag="VFQ35_24560"
     CDS             2874..>3567
                     /gene="hypD"
                     /locus_tag="VFQ35_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET9933908.1"
                     /translation="MKYVDEYRDAELARRLSAALHEETQGSWTLMEVCGGQTHAIVRF
                     GIDQLLPERIRLIHGPGCPVCVTPLETIDRALRLAAQPEIILCSFGDMLRVPGSTSTL
                     AAERARGADVRVVYSPLEAVEIAASQPDRQVVLLAVGFETTAPANAMAVLEARRRDLQ
                     NFSLLVSHVLVPPALVALLDAPDCTVQGFLAAGHVCTIMGTAEYEPIAARYRVPIVVT
                     GFEPLDILEGILL"
BASE COUNT          557 a         1203 c         1192 g          615 t
ORIGIN      
        1 cggaactggt ggcagcgcgc catccgcacg tggagctctg cgtcacgcac gagctcacgc
       61 cggagctttc ggagctcgta gcgcgcgctg agctcgccgt cttcgtcgac gcccgtcacg
      121 atcgcctgga cgagggtgtg ctgagcgaag cattgaagcc tgcgacaacg agccctctct
      181 cggggcacta cagcaacccg cgctcgctct tggcgctttc gcaagcgctt tacggaaggt
      241 acgcgccggc gtggctcgtc acgttgccgg cacacgacac aggtctcggc gagggcattt
      301 cggagtgcgc tcgccgcacc gtgcccgccg cgctcaggcg agtcgacgag ctcatcagcc
      361 aggcgttgcc gtgacgcgct ggcgcgcgga gttccacggc atcgtgcagg gcgtggggtt
      421 tcgtccgcgc gcatatcgcg aagctcgtga gcgagcgctc tccggctggg tgcagaaccg
      481 cggcggaaga gtcgagctcg aggtagaggg accgagcgcc gcgatcgagg atttcatcga
      541 gaccttgcga cgctcgccgg cgcccgcgcg catcgaacac atcgagcgta tggagctcgc
      601 cgaaacgcac gagacaggct ttcacatcgt cacgagccag agcgccgaag tcgaagcggc
      661 gctaccggcc gatctcgcgc cctgcgcgga gtgcgaacgc gagcttcgcg atccggcgaa
      721 ccggcgctac cgtcatccgt tcacgagctg cgttgcttgc ggcccacgtt acagcatcgc
      781 acggggtctc ccatacgacc gcgagctcac cacgatggcg agctttcctc tgtgcccgac
      841 ctgccgccgc gagtacgaag atccgagcga ccgccgctac cacgccgagc ccatcgcgtg
      901 tcccgagtgc ggcccgcagc tcacactggt tacggccggg ggcacggagc tcgccactgg
      961 tacgagcgcg ctctatgagg cggttcgtcg actgcgcgag ggagagatca tcgcgctgcg
     1021 cggcctcggg ggctatcagc tgctggtcga cgcgcgctcg agctcctcgg tgcggcgtct
     1081 gcgcgaacgt aaacaccgcg aagaaaagcc tctcgcggtg ctgtttgcaa gcctcgccga
     1141 ggttcgcagc gcatgcgaca tctcagcggg cgaagccgag ttgctggcgt cgccggcgcg
     1201 tcccatcgtg ctcgtgaagc gccgaagcgg tgcgcccatc gcacccgacg tcgcccccgg
     1261 gctgtcggag ctcggcgtgt ttctgccgcc cacgccgctg ctctcgctat tgctcgcggc
     1321 gctcgacttt cccatcgtgt gcaccagcgg caacctctcc ggcgagccct tgtgcatcga
     1381 caacaccgaa gcgctcgtcc ggctggcgag cgtcgcagac gcattcttga tccacgatcg
     1441 agtcatcgct cggcccctcg acgactccgt cgtgcgactc ggcgcgcgcg gcctgcagtc
     1501 gctgcgacgt gcgcgcggct atgcgccgct gccggtggca cgcttcgaaa ccggggaagc
     1561 cgtggttgcg ctcggcggct accagaagtc gaccatcgcg ctcgcctgga acggtcgcgc
     1621 cattttgagc cagcacctgg gcgaccatga caacgcgaag agcctcgaac tgttcgagcg
     1681 cactctcgac gatcttctcg cgttttacgg agtgcgaccc gaagtcatcg cttgtgacca
     1741 gcaccccgac tatccctcga gcgcgtgggg cgaagcgctc tcgcgtcgct tgggcgcgcg
     1801 cttgttacgg gtccaacatc accacgcgca cgcggccgcc gtactcgccg agcacggggt
     1861 cgatcagcga gcgctcgcgt tgagctggga cggcaccggc ctcggcagcg acggaaccat
     1921 ttggggcgga gaagcgctgg tggtgcaggg cgcgcgcttc caacgtttgg gtcatctggg
     1981 caccttcccg ctgctcggcg gcgaccaggc cgctcgtgag ccaaggcgct gcgcgctcgg
     2041 cttgctgttt gcaacggcgc ctgaacgcgc ccgagacttc gcgcacgagc acttcagagg
     2101 cgaagccgca caggtcctct tggcggcgct cgaacgtcgt gtgaatgcgc cgctcacgag
     2161 ctctttgggt cgtctattcg atgccgtcgc gtcgctgctc ggcattcgcc aacgctgctc
     2221 gtacgaaggg caagccgcga tggagttgga ggcgctggct cgccgcgagc acgccgcggg
     2281 cggctatgcg ttgccgttct cggcaggcgc actcttcgtc gcagatctcg gcgcgttggt
     2341 cgagcaagtc acggcagacc tggcgcggcg cgagagcccg gcccgcatcg cggctcgctt
     2401 ccagaacgcc ctggtcggct acggggtcgc gctcgcggca cgagccggct tagaatgcgt
     2461 cgtgctcggc ggcggttgct tccaaaacca atacttgcgc gacgagctcg cgcgtcgcct
     2521 gacggaacta gggcatcgcg tcttgacggc ggacaccgtg ccgaccaacg atggcggcct
     2581 cgcactcggt caagcctggg tggcgagcca acaatctcgg gactgacgga gcgaaacatg
     2641 tgcttgggca tccccggacg actcgtgaag cggaccaggg ctgagccgcc tttcggcagc
     2701 gttgcgttcg ctggcgtcga acgcgaaatt tgtctcgctt acacgcccga agccgagcca
     2761 ggagactacc tcatcgtgca tgctggcttc gccatcagca tcgtcgacgc ggcggaagcc
     2821 gaagccacgc tggatgcctt acgcgaactc gaagtgacgg aaccggtgcc tccatgaagt
     2881 acgtcgacga ataccgtgac gcggagctcg cgcgtcggtt gtccgccgcg ctccacgagg
     2941 agacgcaggg cagctggacg ctgatggaag tgtgcggcgg acagacccat gccatcgtgc
     3001 gcttcggcat cgaccaactc ctgccggagc gtatccggtt gattcacggt cccggttgcc
     3061 cggtgtgtgt taccccgctc gaaaccatcg accgcgcgct ccgcctcgcg gcgcagccgg
     3121 agatcatcct gtgctcgttc ggcgacatgc tgcgcgtgcc gggcagcacg agcactctcg
     3181 ccgcggagcg cgcgcgcggc gccgatgtac gcgtggtgta ttctccgctc gaagccgtcg
     3241 agattgcggc ttcgcagcca gatcggcaag tcgtgttgtt ggcggtgggc ttcgaaacga
     3301 cggcgcctgc gaacgcgatg gcggtgctcg aggcgcgccg gcgcgatctc caaaacttct
     3361 cgctgctcgt gtcgcacgta ctggttccgc cggcgctcgt ggcgctgctc gacgctcccg
     3421 attgcacggt gcagggattt ttggccgcgg gacacgtgtg cacgatcatg ggcaccgcgg
     3481 agtacgagcc catcgccgcg cgctatcggg tcccaatcgt agtgaccggc ttcgaaccgc
     3541 tagacattct cgaggggatt ttgttgt
//
LOCUS       DASTKS010001155         5434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1301491, whole genome shotgun sequence.
ACCESSION   DASTKS010001155 DASTKS010000000
VERSION     DASTKS010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301491"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFQ35_24565"
     CDS             <1..1007
                     /locus_tag="VFQ35_24565"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9933909.1"
                     /translation="RRLGTSESFDRLVDLLSQFVSQVTTYRWMAVFRHAPQRLGMHTN
                     PQSRESALAEAAIALHCNAGLPTVTIEDEDAVADAEGPPPMVEKVHFGEETIATFALA
                     PRAPSHPNDPVLVKTIASELGGALRMATLVEESRSMATTDTLTGLLNRRAFVDTATRE
                     VVRTSRYKDRFSIILMDVDHFKLINDRRGHAGGDAVLASVARLLATCVRSCDVVARWG
                     GEEFVVALPSTGLEGAAEVAERLRSQLESREIIDTNGERVPVTASFGVAELSPGEKLD
                     QLIDRSDRGMYAAKTAGRNRVFLARGTSLVPGPLLRSTEPKPESSAHGSTRPSQRAAS
                     "
     gene            complement(989..1627)
                     /locus_tag="VFQ35_24570"
     CDS             complement(989..1627)
                     /locus_tag="VFQ35_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9933910.1"
                     /translation="MKTLAALTFGLGAAFAAGCGTNKPELPSGVSAPASCVALDYPDG
                     PYGTEAGAAVQNVCLRGWLAPERTAHDESSLEPIRLSDFYDPDGQRGIELLLVNTAAV
                     WCSACRIEHEDLPARAATYVPKGLRIVGSLFQDQKQNPASVDDLKRWVETFGTNFPMS
                     LDPDYAFGLYASAETSPLNLLIDPRSMKILKKYIGDQSAVMWPYIDAQLAAR"
     gene            complement(1624..2535)
                     /locus_tag="VFQ35_24575"
     CDS             complement(1624..2535)
                     /locus_tag="VFQ35_24575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933911.1"
                     /translation="MQRLPLLFTLVVALAGCSNAPAAADIGDDGGKGGGAGASSGGAG
                     GTGGKAQCDPGPGYGEAVEAQALESVSATLLDADGEPVADEVFFVCGVDLCSPPARTG
                     ADGRVTVSPATPLVEKKPAAKFGEGILTPRFAVLLPRESVIDLGVVHTARLPALSTGA
                     PLVPGSEASSGGLTLMPSAGTQIKIDQLTYESAAEQAFRAVKVPLDRAPELIDASVGL
                     EVVYGATPVETRFCPPAKLRIENTEGWTPNTDVEVLLHGVAIEEEFAPYGGWAKVSDA
                     RVSADGLSIETTSPGLPVLGVIGLRRK"
     gene            complement(2622..4751)
                     /locus_tag="VFQ35_24580"
     CDS             complement(2622..4751)
                     /locus_tag="VFQ35_24580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HET9933912.1"
                     /translation="MQTRFRPVLVALGSLLFANASLAQTPAAPPKPARPEAAPPAEAT
                     PPPTPAPADVAPAAAEPAPAELPPAAAPAAPPAANRPSLSPLPAFPEPSNDAGALTGM
                     GRERPTAARKTDDTDVFAEDWWSHARPILELHGNFRVRAEMFYRFSLGRIDDPSQTLW
                     PRPPDNPYTDAIGPTARYDASLCTYEESGRGASTDTQNLTRCKNNTQAGANIRFRLNP
                     ELHISDNLRVISQIDLLDNVVLGSTSSGYSVTPSQTGGYVNGSRSGYEPLGFSDQTTS
                     PPRSGINSLRDSVHVKRAWAEYSTPVGEARFGRMPNHWGLGMVNHAGDGYDDDYQSTI
                     DRIQFVAGIKPLELYIAGAWDFPNEGPTSETIGAPAAQPYDLAQLDDVDQYVLSVVKK
                     KSPELTRLALSRGDLVINGGLQLTYRKQLLDLGVATVPCQTGAASVGCQLNSANTGES
                     VYVRRDAHYYLPDLWLQVLYKQFRFEAEVATLQGSIGNISTTTAKPTANGKYTLASWG
                     FVAELEQRLVENRLKLGFGTGWASGDADVDGLSPTGLTESGSAVQSQHGDHKISTFRF
                     NPAYRVDLILNRNILSRVQGSYYFRPSLGYDFLRDPSGQRLGGTVAAIWTRASEFVQA
                     PGHARDLGIELNGGVYFQSKDGALNDDPNRMGGFYARLEYGILFPMSGLGYPSAQASR
                     INNQAGTTTAADVSTAQILRLYLGAMF"
     gene            complement(4870..>5434)
                     /locus_tag="VFQ35_24585"
     CDS             complement(4870..>5434)
                     /locus_tag="VFQ35_24585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9933913.1"
                     /translation="KPANVLIRPDGVAKLTDFGIARLPESAGKATGGMVAGTGAYMSP
                     EQVRGDELDTRADLYSAAIVFYEMLTGWTPFDSTERDELMLRAAQLDEPPPPLTTRLT
                     GLPPEIDVVMARALAKDRRHRFGSAIEFGEALRQALRQVPSQSWAAQQDFAAMARTIS
                     QAMPAFSMDVQAEAQRLRTAMMTPERR"
BASE COUNT          866 a         1759 c         1890 g          919 t
ORIGIN      
        1 tccggcgtct cggaacctcg gagtcgttcg accgactcgt ggaccttctg tcgcagttcg
       61 tctcacaagt cacgacgtac cgctggatgg cggtgtttcg gcatgccccg cagcgtctcg
      121 gcatgcacac caatcctcag tcccgcgaga gcgctttggc ggaggcggcg atcgcgctgc
      181 actgcaacgc tggcttaccc accgtcacga tcgaagacga agacgcggtc gcggacgcgg
      241 aggggccacc tccgatggtc gagaaggtgc acttcggcga agagaccatt gcgaccttcg
      301 cgctggcgcc gcgcgcaccg tcgcacccga acgatcccgt tctggtgaag accatcgcga
      361 gcgagctcgg tggcgcgctg cgcatggcga cgctggtcga agagtcgcgc tcgatggcca
      421 ccaccgacac gctgaccggt cttctcaatc ggcgggcctt cgtcgacacc gcgacgcgcg
      481 aggtggtgcg cacgagccgc tacaaggatc gcttctccat catcttgatg gacgtcgacc
      541 atttcaagct catcaatgac cgccggggtc acgccggcgg cgacgccgtc ctcgcctcgg
      601 tggcgcgcct gctcgcgacc tgcgtgcgct cgtgcgacgt ggtcgcgcgc tggggcggcg
      661 aagagttcgt cgtcgcgctg ccgtcgactg ggctcgaagg cgcggccgag gtcgccgagc
      721 ggctgcgcag ccagctcgag agccgtgaga tcatcgacac caacggcgag cgcgtgccgg
      781 tcacggcaag cttcggtgtc gccgagctca gcccaggaga gaagctcgac cagctcatcg
      841 atcgctcgga tcgtggcatg tatgccgcga agaccgcagg gcgcaatcgg gtgttcttgg
      901 cgcgcggcac gtcactcgtg ccggggccgc tcttgcgctc caccgagcca aagcccgaat
      961 cgtccgctca cggttcgacc cgcccgagtc aacgcgcggc gagttgagcg tcgatgtacg
     1021 gccacatcac ggcgctctga tcgccgatgt acttcttcaa tatcttcatc gagcgcggat
     1081 cgatgagcag gttcagcggc gacgtttctg ccgacgcata gaggccgaag gcgtagtccg
     1141 ggtccaggct catcgggaag ttggtcccga acgtctcgac ccaacgcttc aaatcgtcga
     1201 ccgacgccgg gttctgcttt tgatcttgga acaggctgcc gacgattctg agccctttcg
     1261 gcacgtacgt cgccgcgcgc gcgggtaaat cttcgtgctc gatgcgacac gccgagcacc
     1321 acacggccgc ggtgttcacg agcaggagct cgatgccgcg ctgaccatcg gggtcgtaga
     1381 agtcggagag ccgaatcggc tcgagcgagc tctcgtcgtg ggccgttcgc tccggcgcga
     1441 gccagccgcg cagacacacg ttttgaaccg ccgcccctgc ttcggttccg tacggaccgt
     1501 cggggtagtc gagcgcgacg cacgaagccg gcgcgctcac tccgctcgga agctccggtt
     1561 tgttggtgcc gcagcccgcc gcgaacgccg ctccgagccc gaaggtgagg gcggcgagcg
     1621 tcttcacttg cgccgaagcc cgatgacgcc gagaaccggt aacccagggc tcgtggtttc
     1681 gatgctgagc ccgtccgcac tcacacgagc gtcgctcacc ttggcccaac cgccataggg
     1741 cgcaaattct tcctcgatgg cgacaccgtg caagaggacc tcgacgtcgg tgttgggcgt
     1801 ccaaccttcg gtgttctcga ttcggagctt cgccggagga caaaagcgag tctcgaccgg
     1861 cgtcgcgccg tacacgactt cgagaccgac gctcgcgtcg atgagctcgg gcgctcgatc
     1921 gagcggcact ttgaccgcgc gaaacgcctg ctctgcggcg ctctcgtaag tcagctggtc
     1981 gatcttgatt tgggtgcccg cgctcggcat cagcgtgagt ccccccgagc tcgcttcgga
     2041 gcccggcacg agcggcgctc cggtcgaaag cgccggcagc cgcgccgtgt gcaccacgcc
     2101 gagatcgatg acgctctctc tcggcagcag caccgcgaag cgcggcgtga ggatcccttc
     2161 gccgaatttc gcggcgggct tcttctcgac gagcggcgtc gcggggctca ccgtgacgcg
     2221 cccatcagca ccagtgcgcg cgggcggcga gcacaggtcc acgccgcaca cgaagaagac
     2281 ttcgtcggct acgggctcac cgtccgcgtc gaggagggtc gcgctcacgc tctcgagcgc
     2341 ttgcgcttcg acggcctcac cgtatccagg ccccggatcg cactgcgctt taccgccggt
     2401 gccgcccgcg ccgccagacg aagcgcccgc gcctccgccc ttgccgccat catcgccgat
     2461 atcggccgcg gccggggcgt tggagcaacc ggcgagcgcg acgacgagcg tgaagaggag
     2521 ggggaggcgt tgcacgcgcg gagtataccc cgcgcgatgc acggcgtcag atcacctccg
     2581 aggccgtgtg ctcgcagctt tgcgcgggcg ggaagcccga ctcagaacat cgcgcccagg
     2641 taaaggcgca agatctgagc agtggagacg tcggcggcgg tggtcgtgcc ggcttgatta
     2701 ttgatgcggc tcgcctgcgc ggagggatag ccgaggccgc tcatcgggaa gaggatgccg
     2761 tactcgaggc gcgcgtagaa gccgcccatc cggttcgggt cgtcgttgag cgcgccgtcc
     2821 ttcgactgga agtagacgcc gccgttcaac tcgatgccga ggtcacgcgc gtggcccgga
     2881 gcctggacga actcgctcgc gcgagtccaa atggcggcca ccgtgccgcc caagcgctgc
     2941 ccgctcgggt cgcgcaagaa gtcgtaaccg aggctcggac ggaagtagta gctgccctga
     3001 acccgtgaca agatgttgcg gttcaggatc aaatcgacgc ggtacgcggg gttgaagcgg
     3061 aacgtcgaga tcttgtggtc accgtgctgc gattgcacgg ccgagcccga ttcggtcaaa
     3121 ccggtcggcg agaggccgtc cacgtcggcg tcgcccgacg cccagccggt gccgaagccg
     3181 agcttcaagc ggttctcgac cagccgttgc tcgagctccg cgacgaagcc ccaggatgcg
     3241 agggtgtact ttccgttcgc cgtcggcttg gcagtggtcg tcgaaatgtt gccgatgctg
     3301 ccctgaaggg tcgcgacttc cgcctcgaag cggaactgct tgtagagcac ctgcaaccag
     3361 agatcgggca ggtagtagtg ggcgtcacgg cgaacgtaaa ccgactcgcc ggtgttggcc
     3421 gagttcaact gacagccgac gctcgcggcg ccggtctgac atgggaccgt cgccacgcct
     3481 aggtcgagta gctgcttgcg gtaggtgagc tgcaagccgc cgttgatcac gaggtcgccg
     3541 cgcgagagtg cgagccgagt gagctcgggg ctcttcttct tcacgacgct gagcacgtac
     3601 tggtcgacgt cgtcgagctg ggcgaggtcg tacggctgcg ctgcgggcgc cccgatggtt
     3661 tcgctggttg ggccttcgtt cgggaagtcc caagcgccgg cgatgtagag ctcgagcggc
     3721 ttgatgccgg cgacgaactg gatgcgatcg atcgtcgact ggtagtcgtc gtcgtagccg
     3781 tcgccggcgt ggttcaccat gccgaggccc cagtggttcg gcatgcgacc gaagcgcgcc
     3841 tcaccgaccg gagtcgaata ctcggcccaa gcgcgcttca cgtggacgct atcgcgcagc
     3901 gagttgatgc cggagcgcgg gggcgacgtc gtttgatcgg agaagccgag cggctcgtag
     3961 ccactgcgcg agccgttgac gtatccgccg gtctgggagg gtgtcaccga gtagccgctc
     4021 gacgtcgagc cgagcacgac attgtcgagc agatcgatct gcgagatgac gcgcaggtta
     4081 tccgagatgt gaagctcggg gttcaaacgg aaccggatgt tggcccccgc ctgcgtgttg
     4141 tttttgcagc gagtgaggtt ctgcgtgtcg gtgctcgcgc cgcgccccga ctcctcgtag
     4201 gtgcagaggc tcgcgtcgta gcgcgcggtc gggccgatcg cgtcggtgta ggggttgtcc
     4261 ggcggtcgcg gccacagcgt ctgtgacgga tcgtcgatgc ggccgagtga gaagcgatag
     4321 aacatctccg cgcggacgcg gaagttgccg tgaagctcga ggatggggcg cgcgtggctc
     4381 caccagtctt cggcgaagac gtccgtgtcg tcggtcttgc gtgcggcggt cgggcgctcg
     4441 cggcccatac ccgtgagcgc accggcgtcg ttgctcggct cgggaaacgc aggcagaggg
     4501 ctcagcgacg gacgattggc ggcagggggc gcggcgggag cggccgcggg cggcagctcg
     4561 gccggcgccg gctccgcagc ggcgggagct acgtccgcgg gcgcgggcgt ggggggcggc
     4621 gtcgcctcgg cgggcggtgc tgcctctggc cgagcgggct tcggtggggc tgcgggcgtc
     4681 tgcgcgagtg acgcgtttgc gaacagcagc gagccgagcg ctaccaaaac cgggcgaaag
     4741 cgagtctgca tcgtcagcat ccggggctta cggcccgaaa agccggggta agtccgtgcg
     4801 gtcctttcgc atgcggggca ggggagggca aggggagact tggggagggt ccggcttcgt
     4861 gaccgcttct cagcgccgct ctggcgtcat catcgcagtt cgcaagcgtt gggcttcggc
     4921 ctgaacgtcc atggagaacg ccggcatcgc ctgcgaaatc gtgcgcgcca tggctgcaaa
     4981 gtcctgctgc gccgcccacg actggctcgg cacttgtcga agtgcttgcc gaagggcctc
     5041 cccgaattcg atggccgaac cgaaccggtg ccgcctgtct ttggcgagag cgcgcgccat
     5101 caccacgtcg atctcgggcg gcagtccggt gaggcgagtc gtgagtggcg gcggaggctc
     5161 gtcgagctgt gccgcgcgca gcatcaattc gtcgcgctcg gtggagtcga atggcgtcca
     5221 cccggtcagc atctcgtaaa agacgatcgc ggccgaatac agatctgcgc gggtatcgag
     5281 ctcgtcaccg cgcacctgtt ccggagacat atacgccccg gtcccagcga ccatgccgcc
     5341 cgtcgccttg ccggcgcttt caggcaaccg cgcgatgccg aaatccgtca gctttgcgac
     5401 cccgtcgggg cgaatgagca cgttcgcggg cttc
//
LOCUS       DASTKS010001156         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_629439, whole genome shotgun sequence.
ACCESSION   DASTKS010001156 DASTKS010000000
VERSION     DASTKS010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629439"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFQ35_24590"
     CDS             <1..795
                     /locus_tag="VFQ35_24590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9933914.1"
                     /translation="VRLQGGRAVHVPLGSEDFSIDFDRLRGAISPRTRLVIVNFPHNP
                     SGAVLSSRDLDELERCLRASNAFLLSDEVYEHIVFDARPHQSVLRNHELARRSFVVGS
                     FGKAYHATGWKVGWVAAPAPLSVELRKVHQFVTFATSTAAQHALADVLESDARHLADL
                     PAFYQQKRDLFRSLLATSRFELLPVAGTYFQLVEYGALSDADDVSFAKRLTVEAGVAA
                     IPISVFYAEPPAQRRVRFCFAKSDELLREAAARLCRLSATGAWSRS"
     gene            792..1331
                     /locus_tag="VFQ35_24595"
     CDS             792..1331
                     /locus_tag="VFQ35_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933915.1"
                     /translation="MRSSVVLPALALLALGCSRPREAAPNPNGRESAPAVVASAPAAV
                     ASAPAAVASAPAVVASAPAAVASTPKGAASAAEANRRCITPESVGATPLAELKGNGER
                     ERKAIAAGRAELKRVLENPNDFYATVQATDDLVLLELWHQSAFAPENCNDHGNPGGKC
                     RTFGYDPKRDRITSTKFWQ"
     gene            complement(1355..>2364)
                     /locus_tag="VFQ35_24600"
     CDS             complement(1355..>2364)
                     /locus_tag="VFQ35_24600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933916.1"
                     /translation="ARASSVVLEYERGLLDGGGMNVVSYHANFSSTGGRLSSQFGLYY
                     LNFSEGSNPTAHGLAGSATAVFSLPVASRYENGLPRAAIGLYFGSAPTALISGERNYV
                     TVPFVFGFGVPVSPAKAITITPWFELSPSVNLDTVIHSYDFSNVNPLDYYDPATGTVK
                     KLTASDVEKVIQDSVDLEFGFKVGARGGLDLALHASDYFDFAVNASMSSVGTAFSGTR
                     VVYLGGGFVWRWDDIVPAVLPADKRLLHEACEDVEARFKSCPNSRKWRTPEEIQQQFG
                     PTELKPPTPAPASSAPLPSAPAPIAPAPATTVAAPAPSTPAAPAPTAAPPADGGSFPL
                     Q"
BASE COUNT          417 a          740 c          807 g          400 t
ORIGIN      
        1 gttcggctgc agggcggacg cgcggttcac gttccgctcg ggagcgagga cttttccatc
       61 gatttcgacc ggctgcgagg cgcgatatcg ccacgcacgc gcctcgtcat cgtgaacttt
      121 ccgcacaatc cgagcggcgc cgtgctttcg tcgcgcgacc tcgatgagct cgagcgctgc
      181 ttgcgggcat ccaatgcttt tttgctctcg gacgaagtgt acgagcacat cgtgttcgac
      241 gcgcggccgc accagagcgt gttgcggaac cacgagcttg cacgccggag cttcgtcgtc
      301 ggatcgttcg ggaaggcgta tcacgcgacg ggatggaagg tcgggtgggt ggcggcgccg
      361 gcgccgctca gcgtcgagct ccgcaaggtg catcagttcg tcaccttcgc gacgtcgacc
      421 gcggcgcaac atgcgctcgc cgacgtcctc gagagcgacg cccgccacct cgccgattta
      481 ccggcctttt accagcaaaa gcgcgacttg ttccggagtc tactcgcgac ttcgcgtttc
      541 gagctcttac cggtggcagg cacgtatttt cagctcgtcg agtacggcgc tctctcggac
      601 gcggatgacg tgagctttgc gaagcggctc accgtcgaag ccggtgtcgc ggcaattccg
      661 atatcggtat tttacgcgga gccaccagcc cagcgccgcg ttcgcttctg ctttgccaag
      721 agcgacgagc tcctgagaga agcagcggct cgcctctgcc ggctcagcgc gacgggcgct
      781 tggagccgct cgtgagatct tccgtcgtgc tgccggcctt ggcgctatta gcgctcgggt
      841 gctcacggcc gcgagaagcc gcgccgaatc cgaatggtcg agagagtgcg ccggcggtcg
      901 ttgcgagtgc gccggcggcc gttgcgagcg cgccggcagc cgttgcgagt gcgccggcgg
      961 tcgttgcgag tgcgccggca gccgttgcga gcacgccaaa aggtgcggcc tcggctgcgg
     1021 aggcgaatcg ccgctgcatt acgccggagt cagtgggcgc gacgccgctc gccgagctca
     1081 agggtaatgg agaacgcgag cgcaaagcga tcgccgccgg gcgcgcggag ctcaagcgcg
     1141 ttctcgaaaa cccaaacgac ttctacgcga ccgtacaggc aacggacgat ctcgtgctgc
     1201 tcgagctatg gcaccagagc gcgttcgcgc cggagaactg caacgaccat ggcaaccccg
     1261 gcggcaagtg ccgaacgttc ggctacgacc ccaagcggga tcgcatcacc agcaccaagt
     1321 tctggcagtg actcgacgcg gatccgtcac gaactcactg cagcggaaag cttcccccgt
     1381 cggcgggagg cgcggcggtg ggagccggcg ccgcaggagt cgacggtgcg ggggccgcga
     1441 cggtggtagc aggagcgggg gcgatcggag ctggagcgct cggtagaggc gccgagctcg
     1501 ccggagcggg cgttggcggc ttgagctcgg tcgggccgaa ttgctgctgg atttcctcgg
     1561 gcgtgcgcca ttttcgcgag ttcgggcaag acttgaagcg cgcttcgacg tcctcgcagg
     1621 cctcgtggag caggcgtttg tcggcgggca agaccgccgg gacgatgtcg tcccaacgcc
     1681 acacgaagcc gccacccaag tacacgacgc gagtccccga gaacgcggtg cccaccgagg
     1741 acatgctcgc gttcaccgcg aagtcgaagt agtccgaagc atgcagcgcg aggtctaaac
     1801 cgccgcgagc gccgactttg aaaccgaact cgaggtcgac cgaatcttga atcaccttct
     1861 ccacgtcgga ggcggtgagc ttcttcacgg tgcctgtagc cgggtcgtag tagtcgagcg
     1921 ggttgacgtt gctgaaatcg tacgagtgga tcaccgtgtc caagttcacg ctcggagaga
     1981 gctcgaacca gggcgtaatc gtgatcgctt tagcgggaga gaccggcacg ccgaagccga
     2041 agacgaacgg aaccgtaacg tagttgcgct ctccgctgat gagtgccgtc ggggcgctac
     2101 cgaagtagag accaatcgcc gcgcggggga ggccattttc gtaacggcta gcgaccggca
     2161 acgagaacac cgcggtggcg ctgcccgcga gaccatgtgc cgtcgggttc gagccttcgc
     2221 tgaagttcag gtaatacagg ccgaactgcg aactcaggcg accgccggtc gagctgaagt
     2281 tcgcgtggta gctgacgacg ttcatgccgc cgccatcgag caagccgcgc tcgtactcga
     2341 ggacgacgct cgaagcgcgt gcat
//
LOCUS       DASTKS010001157         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_629510, whole genome shotgun sequence.
ACCESSION   DASTKS010001157 DASTKS010000000
VERSION     DASTKS010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629510"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFQ35_24605"
     CDS             complement(<1..422)
                     /locus_tag="VFQ35_24605"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET9933917.1"
                     /translation="MANLKFYMTPGSCSTGIHLLLEELELFFEAHVVNLVTGENLRPE
                     YLAINPRGSVPTLVRGDGTALTDFISIARFLAETHPERKLLPDSEADRARVFELMQYA
                     IGVVHGEGFTRVFVSERYASNAETKEATESEGRQIVRKG"
     gene            687..1037
                     /locus_tag="VFQ35_24610"
     CDS             687..1037
                     /locus_tag="VFQ35_24610"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9933918.1"
                     /translation="MTFVPVCPASAVREGTMGLFQVGKKSVLLVWPAGGELKAFRGRC
                     PHADMPLTDATFDGQKVTCALHGWGFDCKTGKCVTHFVNNALHPYEVRVEGEEVLVDV
                     GAVRGAGQAGTAPP"
     gene            complement(1117..>2757)
                     /locus_tag="VFQ35_24615"
     CDS             complement(1117..>2757)
                     /locus_tag="VFQ35_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933919.1"
                     /translation="GGGGVGGGGDAGHDAGASGDAGSVSGGAAGSSGAGGAAGETGGV
                     NGGPSAGAGGELAGSGGDATDFASTVRDGVASVQQKPRPAFGALRVIAQGARRYAVES
                     RRDVEPGPFNLPWRSRFRVAAYDGDAEVWAWTAEPDDCIGDVAMHPSGDVTLSLERQQ
                     PTSSAYQLVRLSSDGAELGRTTLAEPQTAPLTDFGPNDARPLFRMKSEFADATTAGSV
                     ALLPNGEDVVAAILSFVAVPEGEAPTTRRALELEALAWNGASYDERWSRVVEGTHAAE
                     PALWAYDEFRWREQAIRPFLAYDDVTGDWLVGRAWNTTRCLANVSTFAEFTNMQCVFE
                     AVGSSENELLPLAVTRFDATGRRLGTRIIATDPDAAEQVPFGLVAHGGELAVAGAVVR
                     TNEDGSRRTYPDPKGFVDYDGYIARYDTEGHRLAGRDFNLGRGDVLAALRWTPSGILA
                     VGSAGWDRWQGGMSVSRGADPLFAWLSFDGSEAEARVIALSDGSRHFTLHDIVIAGSD
                     ALGFGFSDAPMTHSGDGNNAAARTFGPLRVRLARANNL"
BASE COUNT          485 a          972 c          842 g          458 t
ORIGIN      
        1 ccctttcgaa cgatctgccg accctcactc tcggtcgcct ccttcgtttc cgcgttcgac
       61 gcatagcgct cggacacgaa cactctcgtg aagccttcgc catgaaccac gccaatggcg
      121 tactgcatga gctcgaacac gcgcgcgcgg tccgcctccg aatcggggag cagcttgcgt
      181 tcgggatggg tctcggcaag aaagcgagcg atcgagatga agtcggtgag cgcggtcccg
      241 tcgccccgta cgagcgtcgg gacgctaccc cgtggattga tggcgaggta ttcgggcctc
      301 aggttttcgc ccgtcaccaa gttgaccacg tgcgcctcga agaagagctc gagctcctcg
      361 aggagaaggt gaatgcccgt gctgcacgag cccggggtca tgtaaaactt caggtttgcc
      421 acgatgaacg ttgctcggtg cccgcgagag gatccccggc agcgagacac gaagcaaaga
      481 ccctgcctga ccgctctgcg gctgcggggc gctctcaacg ccaaagccca atacggaaag
      541 gcgacacact tcgaaggctc tccgacctcc ttgtcgtgtt tgcgacaacg cgacgtcgac
      601 gagcaccgcg cccagcgcga atccacgcgc ctcgttcgcg gcgcgctagt tgctacccgc
      661 ccttgtctca ctcagaaagg cgagcgatga cgttcgtacc cgtgtgtccc gcgagtgccg
      721 tccgtgaagg cacgatgggg ctcttccagg tcggaaaaaa gagcgtgctc ttggtttggc
      781 ccgccggagg cgagctcaag gcgtttcgcg gccgctgccc tcacgccgac atgcctctga
      841 ccgatgcgac cttcgacgga cagaaggtga cgtgcgcgct gcatggttgg ggcttcgact
      901 gcaagactgg caagtgcgtt acccacttcg tcaacaacgc cctgcaccca tatgaagtac
      961 gggtagaagg cgaagaagtc ttggtcgacg tcggcgcggt cagaggcgca ggacaggcgg
     1021 gtaccgcgcc gccctgattt cgactcgaag cttcgttcac atcggagccg ccagcggcgc
     1081 tgttctccgc ttctcttgcc tcgcactcgt tcgtcgctag aggttgttgg cgcgcgctag
     1141 gcgaactcgg agagggccga aggttcgcgc cgccgcattg ttgccatctc cggaatgcgt
     1201 cattggcgcg tcggagaagc cgaagcccaa ggcgtcgctt cccgcgatca cgatgtcgtg
     1261 caacgtgaaa tgccggctcc catcgctgag cgcaatcacg cgtgcttcgg cttccgaccc
     1321 gtcgaacgag agccaggcga agagcggatc cgcgccgcgc gaaacgctca ttccgccctg
     1381 ccaacgatcc cagccggcgg agccgaccgc caaaattccg ctcggcgtcc agcgcagcgc
     1441 cgcgagcacg tcgccgcgac ccagattgaa gtccctcccc gcgagacgat gaccctctgt
     1501 atcgtaccgc gcgatgtacc cgtcgtagtc gacgaaacct ttcgggtcgg ggtacgtgcg
     1561 gcgcgagcca tcttcgttcg tgcgcacgac cgctcccgcg acggcgagct ctccaccatg
     1621 tgccacgaga ccaaacggca cttgttcagc ggcgtcaggg tcggtcgcga tgattcgggt
     1681 accgaggcgg cgacccgttg catcgaagcg cgtcaccgcg agcggcaaga gctcgttttc
     1741 cgaagagccg accgcctcga acacgcactg catgttggtg aattcggcga aggtgctcac
     1801 gttggcgagg caccgcgtcg tattccaagc gcgcccgacg agccaatcac cggtcacgtc
     1861 gtcgtacgcc aagaaaggac gaatcgcctg ttcgcgccaa cgaaactcgt cataagccca
     1921 caatgctggt tccgccgcgt gcgtcccctc gacgacgcgg ctccagcgtt cgtcgtagct
     1981 cgcgccgttc cacgcgagcg cttcgagctc gagcgcgcgg cgtgtagtcg gcgcctcacc
     2041 ttccggcacc gcaacgaaag acaagatcgc ggcgacgacg tcttcaccgt tcggaagcag
     2101 cgcgacagac ccagcggtgg tcgcgtcggc gaactcggac ttcatccgaa acagcgggcg
     2161 agcatcgttc gggccaaagt cggtgagcgg agccgtctgc ggttcggcga gcgtcgtgcg
     2221 gccgagctcc gcgccgtcgc tcgacaagcg caccagttga tatgcgctac tcgtcggctg
     2281 ctgtcgttcg agcgacagcg tgacgtcgcc agacgggtgc atcgccacgt ccccaataca
     2341 gtcgtccggc tcggctgtcc aagcccagac ctccgcgtcg ccatcgtacg ccgcaactcg
     2401 aaaacgcgac cgccagggca agttgaacgg ccccggctct acgtcgcgtc gcgactcgac
     2461 cgcgtaacgc ctcgcgccct gtgcgatgac ccgcaacgct ccgaacgcag ggcgcggctt
     2521 ttgctgcacg ctcgcaacgc cgtcgcgcac cgtggaagcg aaatccgtag cgtcgccgcc
     2581 gctgcccgcc aactcgccgc ccgccccggc gctcggacca ccgttcacgc cgccggtctc
     2641 gcccgcagcg ccgcccgctc cggatgagcc agcggcaccg ccgctcacac ttccggcgtc
     2701 acctgacgcg ccggcgtcgt gacctgcgtc gccccccccc cccacccccc ccccccc
//
LOCUS       DASTKS010001158         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_406097, whole genome shotgun sequence.
ACCESSION   DASTKS010001158 DASTKS010000000
VERSION     DASTKS010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406097"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VFQ35_24620"
     CDS             <1..762
                     /locus_tag="VFQ35_24620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933920.1"
                     /translation="PEQARGEKVDARCDIYALGVLCYQMMTGQLPFDAETTVAILMAH
                     LIQSAEKPSEIVPELSPEFDAPLLRMLEKDPAKRPSSAGAAIAEIRSAAERAGLSVLP
                     GPPKLARPEPETSGEFPAEIESTLLASPPTPGGSSRGAERRKFLLFGAGALFVGATFT
                     WLFAHASHPSDARAPVATPEARLSSTLVSAPAPPVSATASAAPAIVEAPSAAPSASAP
                     SKRVRPVTNVERGKKATSEAPASATPHDLESPFGP"
     gene            752..3316
                     /locus_tag="VFQ35_24625"
     CDS             752..3316
                     /locus_tag="VFQ35_24625"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9933921.1"
                     /translation="MVRKGAARWLATLLGVCALGCTDLPEIDTGECGNGVREDRETCD
                     TFALNPGGRCRPKGSVGECHLDCRLQSDGTRGTCPEGWGCDRDGLCRKPVGLGESIEL
                     PLKGVESLASGDYDGDGRSDLVTREPVDAALRGKPTLHYLDENGAIRMSLPFPKAVGE
                     PFVGDIDGDRLDDFVFTTFLVGVLRGRPDRNWVPETFSSYRVPDSHLRVVGVAEDAVI
                     SNAVNVVVFGTLQDRIGLFVPLESGALQLMAPLPASVEMIAGDIRTGNIVDGVDSPCN
                     EVVVAYRGASSFSAFDVCDWDSTIGFPAWKTAAKEQKVALQPPAEIQSTLVADLNGDD
                     HLDVLIGTNAGVYAAYGDGKKLATATRFFLHRADQPQTPIDIPMPLAVGEFSGDRTPD
                     FVFPESVLCSLAHAEHPSGYPVYGQVAVIESVRWTEALIADMNGNGKPDIVAASRDGL
                     GIQFFNGTDGLFVAGSSIPTAGPVEHLSIGDFDGDQLNDVAFIEHAVSSSESDSLRIA
                     FGSPAGPPQAPITVAQMDAAEQVTAFADAGFWSLAVASSDRRDGTMTGQVATLIGGDR
                     LPLALHALVRLGEDGSIARSLAYAVSVGAFGGPGKHDLVALSHREDTFEFWLIPAIND
                     NASAPMRLSGALPRGFEPSALDAGAISRLAVAATALDVDGDGVDESLWMMPAAEGTRC
                     GLFWFDVDQGARALLPRGVAELTDPCARASIKAKDLDGDGRSDLLLASSDLSETTGAL
                     TVLFNDGNGKFKTPVKVSMPGEVPRAFTFLRRTQASPGMFAYVTGNALELVGSTSDLT
                     ASVARGAEFFDVRQRLLALERGTAVVSGDFNGDGVEDLAVADAGKVRLSLTQLELP"
BASE COUNT          539 a         1135 c         1153 g          610 t
ORIGIN      
        1 cccgagcagg cgcgcggcga gaaggtggat gcgcgctgcg acatctacgc gctcggtgtc
       61 ctttgttacc agatgatgac ggggcagctc ccgttcgacg ccgagacgac ggtagccatc
      121 ttgatggcgc acttgattca atccgcagaa aagccctcgg agatcgtgcc ggagctatcg
      181 cccgagttcg acgccccgct gctccggatg ctggagaagg atccggcgaa gcgcccgagc
      241 agcgcgggcg ctgcgatcgc agaaatccgt agcgcggcag agcgcgccgg cctttcggtg
      301 ctgcccggcc cccccaaact cgcgcggccc gagcccgaga cctccgggga gtttccggca
      361 gaaatcgagt cgacgttgct cgcgagtcca cccacgccgg ggggatcgtc gcgcggcgcc
      421 gagcggcgga agttcctgct cttcggagcg ggcgcgctgt tcgtcggcgc gaccttcacg
      481 tggctgttcg cgcacgcttc gcacccgagc gatgcgcggg cgccggtcgc gacgccagaa
      541 gcgcgacttt cgtcgaccct cgtttcggcc ccggcaccac cggtgagcgc gaccgcgagc
      601 gctgcgcccg cgatcgtcga agctccttcg gccgctccga gcgcttcggc gccatccaag
      661 cgcgtccgcc cggtgaccaa tgtcgaacgc ggcaagaagg cgacgtcaga ggctccggcg
      721 tcggcgaccc ctcacgatct ggagagcccg tttggtccgt aagggcgccg cacgttggct
      781 cgccacgctc ctcggcgtgt gcgcgctcgg ctgcaccgat ttaccagaga tcgacactgg
      841 tgaatgcggc aacggcgtgc gagaggaccg cgagacttgc gacacgttcg ctctcaaccc
      901 gggcgggagg tgtcggccca aaggcagcgt gggagagtgt cacctcgact gccggctcca
      961 gagcgacggc acgcggggga cctgccccga ggggtggggc tgcgaccgcg atggcctgtg
     1021 ccgcaagccg gtcgggctcg gcgagagcat cgagctcccg ttgaaaggcg tcgagtcact
     1081 cgcttcgggc gattacgatg gcgacggccg ctcggatctc gtcacgcgtg aaccagtcga
     1141 cgccgcgctg cgcggtaagc ccacgctcca ctacttggac gagaacgggg cgattcgcat
     1201 gtcgctgcct ttcccgaagg cggtgggtga acccttcgtt ggcgacatcg acggcgatcg
     1261 gttggacgac ttcgtcttca ccacgtttct cgtcggggtg cttcgcggac ggcccgatcg
     1321 caattgggtg ccagagacgt tcagctcgta ccgagtaccc gactcgcatc tccgcgtggt
     1381 tggcgtcgcc gaggacgcgg tcatttcgaa cgcggtcaac gtcgtggtgt tcggtactct
     1441 gcaggatcgg atcggcctgt tcgtgcccct cgagagcggg gccctgcagc tcatggcgcc
     1501 tctcccggct tccgtcgaga tgatcgcagg cgacatccgg accggaaaca tcgtcgatgg
     1561 cgtggattcc ccctgcaacg aggtggtcgt agcgtatcgc ggcgcaagct cgttctccgc
     1621 cttcgacgtg tgtgattggg actcgaccat cgggtttccc gcgtggaaga cggcagccaa
     1681 ggagcagaag gttgctctgc agccgcccgc cgagatccaa tcgaccctcg ttgccgactt
     1741 gaacggtgac gaccacctcg acgtgctgat cggcaccaac gcaggtgtgt acgcggcgta
     1801 cggcgatgga aagaagctcg ccaccgcgac ccgattcttt ctgcaccgag cggaccagcc
     1861 gcaaacccca atcgatatcc caatgcccct cgccgtgggt gaattcagcg gcgaccggac
     1921 gccagatttc gtcttcccag agtcggtgtt gtgttcgctc gcgcacgcgg agcacccgag
     1981 cggctacccg gtttacggcc aagtcgcggt gatcgagagc gtccgatgga ccgaagcctt
     2041 gatcgccgac atgaacggga acggcaagcc cgatatcgtc gcggcttcgc gggatggcct
     2101 cgggatccaa ttcttcaacg gaaccgacgg gctgttcgtc gccggctcca gcattccgac
     2161 ggcgggaccg gtcgagcacc tgagtatcgg ggatttcgac ggcgaccagc tcaacgacgt
     2221 ggccttcatc gagcacgcgg tgagctcctc cgagtcggac tcgctacgga ttgcgttcgg
     2281 ctcgcctgcg gggcctcctc aagcgccgat cacggtcgct cagatggacg cggccgagca
     2341 agtcaccgcc ttcgccgacg ccggcttttg gagcctcgcg gtcgcttcga gcgaccggcg
     2401 tgatgggacg atgacgggtc aggtcgccac cttgatcgga ggcgatcgct taccgcttgc
     2461 tttgcacgcg ctcgtccgct tgggtgagga cggatccatc gcgaggtcct tggcctacgc
     2521 ggtcagcgtg ggagcctttg gcggtcccgg aaagcacgac ttggtcgcgc tctcgcaccg
     2581 agaggacact ttcgagttct ggttgatccc ggccatcaac gacaatgcct ccgccccgat
     2641 gcggctctcg ggcgcgctcc ctcgggggtt cgagccgagt gcgctcgacg ctggcgccat
     2701 cagtcgtctt gcggttgctg cgaccgcgct cgatgtcgac ggcgacggag tcgacgagtc
     2761 gctctggatg atgcctgccg cagaggggac gcgctgtggt ctgttctggt tcgacgtcga
     2821 ccagggagcc cgcgcgctgc tccctcgcgg agtggccgag ctcacggacc cgtgcgcgcg
     2881 cgcttcgatc aaagccaagg atctggacgg agacgggcgg agtgacctac tgctcgcgag
     2941 ctccgacctt tcggagacga cgggggccct caccgtgctt ttcaatgacg ggaacgggaa
     3001 gttcaagacg cccgtgaaag tctcgatgcc cggcgaggtt ccgcgcgcct ttacgtttct
     3061 ccgacgaact caagcttctc cgggcatgtt tgcctacgtg accgggaacg ctctcgaact
     3121 cgtcggctcg acctccgacc ttacggcgag cgtcgcgcga ggcgccgagt tcttcgatgt
     3181 gcgtcaacga ctgctcgcgc tcgagcgagg gacggccgtc gtgagcggtg acttcaacgg
     3241 cgacggagtc gaggatctgg cggtggcgga cgctggaaag gtccgcctgt cgctcacgca
     3301 gctggagctg ccatgagacg cctcgcgtgg tcttgtgcgc tcgccggtgc caccttcgct
     3361 tcggccgcat ccgcgcagac tcccgctgat ttgtcgcgct cccgcgagct ctttcgcgcc
     3421 ggagccagcg cctacgc
//
LOCUS       DASTKS010001159         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1525524, whole genome shotgun sequence.
ACCESSION   DASTKS010001159 DASTKS010000000
VERSION     DASTKS010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525524"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFQ35_24630"
     CDS             complement(<1..572)
                     /locus_tag="VFQ35_24630"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET9933922.1"
                     /translation="MQRLGIYRESGHEHFYGCLTGAYGIERVLIAYDADEAGSRAAEK
                     LAPELAAMGLSVFRVNFSKGMDANEYAQKLKPADKSLGTALRAAHWMAGSRAVAVPRE
                     LENAQAYNHVAAETNGHGSALRARDVVEVEALPPELVRHEVHERVVANDEQVVGPLSP
                     EVEPSKAEHPIPPPPLVASLADERAVEASV"
     gene            complement(573..956)
                     /locus_tag="VFQ35_24635"
     CDS             complement(573..956)
                     /locus_tag="VFQ35_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933923.1"
                     /translation="MGACQVGGTVIDFVMRVERCSFRLACEMLLKDAPSLAASLEADR
                     PRTTTARLEEIAQADEPDHVVLARVVDFYHATLKSSGEARAYLGSRGIEHGELVETFK
                     LGYANRTLGYRLPPKQVKSGAGIRG"
     gene            complement(963..1139)
                     /locus_tag="VFQ35_24640"
     CDS             complement(963..1139)
                     /locus_tag="VFQ35_24640"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HET9933924.1"
                     /translation="MARIPDEVVERLKREVSVERLVQAKGIELKRHGDNLVGRCPFHD
                     DRTPSLVVTPSKNL"
     gene            1263..1643
                     /locus_tag="VFQ35_24645"
     CDS             1263..1643
                     /locus_tag="VFQ35_24645"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9933925.1"
                     /translation="MPRPSRLLPEPFAPGDESFGQRLARLRKDRGYTQVELAEKIGII
                     QALVSDYERDKLRPYGDMVARFALALDVSADELLGIVPLVKTRATHNRRFLRRMQAMD
                     KLPKRDQEALLRTIDAFLDRAKTG"
     gene            1656..1949
                     /locus_tag="VFQ35_24650"
     CDS             1656..1949
                     /locus_tag="VFQ35_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933926.1"
                     /translation="MASRDSIVRDLLEKLRDAPNEELSEIADFAEFLHAKRLKRLAPA
                     PSAPSLDELEARAAAAGLLRLPEPNARRLSTAEVPPVQVGGKPASEIVLEDRR"
     gene            1956..2381
                     /locus_tag="VFQ35_24655"
     CDS             1956..2381
                     /locus_tag="VFQ35_24655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET9933927.1"
                     /translation="MLVYFDTSALVKRYLVETDSPAVEALWNKTTRAVASEILYDEMA
                     ATFARKKRDAPSDAENVERAHATFRAEWLSLRRVAVNDDVHRRVEELLLRHSLRGADA
                     IHLASATLVRDALQQPLTFACADAKLVAAARAEGLDVIP"
     gene            complement(2409..>2601)
                     /locus_tag="VFQ35_24660"
     CDS             complement(2409..>2601)
                     /locus_tag="VFQ35_24660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933928.1"
                     /translation="RGGSAAWSNRDSAERRNLAANANHLADALSRIATTSTHVQPQAA
                     RAFSGGTESRGQFRYLLSS"
BASE COUNT          460 a          917 c          773 g          451 t
ORIGIN      
        1 tccacagagg cttcgacggc gcgctcgtct gcgagagagg cgactaaagg aggaggtggg
       61 atcggatgct ctgccttgct cggctcgacc tcgggcgaca gcggtcccac gacctgctcg
      121 tcgttcgcca cgacgcgctc gtgcacctca tgccgcacca gctcaggcgg cagcgcctcg
      181 acttctacga cgtcgcgtgc gcgcagcgca ctgccgtggc cgttcgtctc ggcggccacg
      241 tggttgtacg cctgcgcgtt ctcgagctcg cgtggcaccg ccacggctcg gcttcccgcc
      301 atccagtgcg ccgcgcgaag cgccgtgccc aagctcttgt ccgcgggttt gagcttctgc
      361 gcgtactcgt tcgcgtccat ccccttcgag aagttcaccc gaaataccga cagccccatc
      421 gcggcgagct cgggggcgag cttctcggca gcgcggctgc ccgcctcgtc cgcgtcgtac
      481 gcgatcagca cgcgctcgat gccatacgca cccgtcaagc acccgtaaaa gtgctcgtgc
      541 ccgctctcgc gatagatgcc gagccgctgc aattacccac gaatccctgc ccccgacttc
      601 acctgcttcg gcggcagccg gtacccgagc gtccgattcg cgtagccaag cttgaaggtc
      661 tctacgagct ccccgtgctc gatgccgcgg ctgccgaggt acgcacgggc ctcgccgctc
      721 gatttcagcg tcgcgtgata gaagtccacc acgcgcgcta gcaccacatg gtcaggctcg
      781 tccgcctgcg cgatctcctc gagtctcgct gtcgtcgtgc gcggccgatc tgcctcgagc
      841 gaagccgcta aagagggagc atccttcagc agcatctcgc acgcgagacg gaacgagcag
      901 cgctccacgc gcatcacgaa gtcgatcact gtcccgccga cctggcatgc gcccatgcag
      961 tgtcacaggt tcttgctcgg cgtcaccacg aggctcggcg tgcggtcgtc gtgaaacgga
     1021 cagcgcccga cgaggttgtc gccatgccgt ttcagctcga tccccttcgc ctgcaccagg
     1081 cgctcgaccg aaacttcgcg cttgagccgc tccaccacct cgtccgggat ccgggccatt
     1141 gcctcgctcc ttcgaagaaa gaccgtcaag ccgaaagtga ttgcgtgacg ccgttcctga
     1201 aatacagttc taaactgtat gcgtcgaggc tatacgaaaa tgcgccccta cactgagctt
     1261 ccatgcctcg cccatcacgc ctgctgccgg agccgttcgc gcccggcgac gagtccttcg
     1321 gacagcggct cgcccgcctc cgcaaagacc gcggctacac tcaggtcgag ctcgctgaga
     1381 agatcggcat catccaggca ctcgtctccg attacgagcg agacaagctc aggccctatg
     1441 gcgacatggt cgcgcgattt gcgctcgccc tcgacgtcag cgccgacgag cttctcggca
     1501 tcgtgccgct cgtgaagacg cgcgccacgc acaatcggcg cttcttgcgg cgcatgcagg
     1561 ccatggacaa gcttcctaag cgcgatcaag aggcgctact gcgcaccata gacgcgttcc
     1621 tcgatcgtgc gaagacaggc tgacaggagg ctcccatggc atcacgagat agcatcgtgc
     1681 gagacctact cgagaagctt cgcgacgcgc ccaacgagga gctttctgag atcgcggact
     1741 tcgcagagtt cctgcacgca aagcgcctca agcgccttgc tccagcgccc tccgcgcctt
     1801 cgctcgacga gctggaagca cgggctgccg cagcgggtct attacgcctg ccggagccaa
     1861 acgcgcgcag gctttcgacc gccgaagtgc cgccggtcca ggtcggcgga aagcccgcct
     1921 ccgagatcgt cctcgaggac cgcaggtaga aacgaatgct cgtctacttc gacacctccg
     1981 ccctcgtgaa gcgctacctc gtcgagaccg actcacccgc cgtcgaagcg ctctggaaca
     2041 agacgacgcg cgccgtggcc tccgagatcc tctatgacga gatggcggcc actttcgcgc
     2101 gcaagaagcg cgacgcgcct tccgacgccg agaacgttga gcgtgcacac gcaaccttca
     2161 gggccgagtg gctcagcttg cggcgagttg ccgtcaacga tgatgtccat cggcgcgtgg
     2221 aagagctgct cttgcggcac agcctccgtg gtgctgacgc cattcacctc gcatccgcaa
     2281 cgctcgtccg cgatgctctg cagcagccgc tcaccttcgc atgcgcagat gccaagctcg
     2341 tcgccgccgc gcgcgccgaa ggactcgacg tcattccgtg attcgcgcag gagtcctact
     2401 cctcgccatc aggaagaaag caaatatcga aactgacctc gagattcagt tccgccagaa
     2461 aaagcccggg cggcttgcgg ctgcacatgc gtggaagtag tagcgattcg gctgagcgcg
     2521 tcggcgagat ggttggcgtt cgccgcgagg ttgcggcgct cggccgaatc gcggttggac
     2581 caagccgcgg agccgccgcg a
//
LOCUS       DASTKS010001160         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1077065, whole genome shotgun sequence.
ACCESSION   DASTKS010001160 DASTKS010000000
VERSION     DASTKS010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077065"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFQ35_24665"
     CDS             <1..283
                     /locus_tag="VFQ35_24665"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HET9933929.1"
                     /translation="GEGIDEETRRDVLKIANETPGVLAVTQLLSLHLGPSSVLLALKV
                     RFQAGASLPDVERTINQVEERIRAAEPRMRRIFVEPDAEYDPSRDPDAI"
     gene            complement(377..1339)
                     /locus_tag="VFQ35_24670"
     CDS             complement(377..1339)
                     /locus_tag="VFQ35_24670"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET9933930.1"
                     /translation="MTALLSWFLGFLKESFTENIGLKALSLSIAVGLFAILYGKQDEQ
                     LRTLPVTVVLRPPAEGSDRELMTAIPPNIHLTLRGPARALDRAAQEGLLPIEIDLRQG
                     NKEQIVFDPREFKLPPDLKVSIIDPPSIDLDWEDVVVREIPVQASISGQPASGYVVKG
                     EPEPDPATLAARGPRSLVEVVQSARLAPFDVSGLSEGVHRRRIALDGPPPRVKFIGPT
                     SVNVTVTIARRLAEVHFDSRPVEIAGVASGTSTPRAVNVVVYGPPEVVHSLKPDQVVP
                     RADLSKLPGLDLKEQRHGAAVVKLSVELTRAEVEIQPPSVNVKW"
     gene            complement(1336..2463)
                     /gene="cdaA"
                     /locus_tag="VFQ35_24675"
     CDS             complement(1336..2463)
                     /gene="cdaA"
                     /locus_tag="VFQ35_24675"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET9933931.1"
                     /translation="MLEVLRSYFEERTFLQLLRDGLDVFIVYYLIYRSLLVVRGTRAM
                     QIALGLGVVLFIYLVATWLQLVTVLTIMSYLVASLITIVVVVFQNDIRRGLQRVGSRA
                     WLGHLARWQESRVIDEVVEAATDLARHRIGALITIEQDANLDEFVGVHKGHTLDAQVT
                     AELLVSLFVPEAVNKLHDGAVIIRNYRIAKAGVFFPMPEGRVVDDSFGSRHRAALGIT
                     EETDAVVVIVSEERGTISFCFNGNIASNLDGPKLKTMLEAIFSPRVRKKSRKVAAQRD
                     AHREPSAESVRPPEPARAPRHVDEDVDHPRISLVADMPTETPAPVRPRSEPPPPPLRA
                     PQPPLPSPATPLRTPVAPRVAPRVEPAEAEPNAQSKRRGDE"
     gene            complement(2456..3325)
                     /gene="folP"
                     /locus_tag="VFQ35_24680"
     CDS             complement(2456..3325)
                     /gene="folP"
                     /locus_tag="VFQ35_24680"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9933932.1"
                     /translation="MGALSIRLSQCRAERGAALMGVLNVTPDSFYDGGRYLDEESARE
                     RVDALIEAGADIIDIGGESTRPGAPAIPAGEQLARIEPALRHAVSRRALVSVDTTDPT
                     VAERALSIGAVCVNDVSCLADERLARACARHSATLIVMHSRGPMANMPGFSSYPDDAY
                     GDVVADVLAEWRAARDRAMQQGLARESIWFDPGIGFAKNARHSFELIRGLGRFLDEGV
                     PVVVGPSRKSFINAVDPAPPDARLGGTIAASLACVARGAAVVRVHDVLEVRQALAVAR
                     GVDRGLSGEVPRA"
     gene            complement(3460..4281)
                     /locus_tag="VFQ35_24685"
     CDS             complement(3460..4281)
                     /locus_tag="VFQ35_24685"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9933933.1"
                     /translation="MGELASLNELEEQLARMLTRRGALERDPEAIALANRYLTGNDRL
                     SPVEQLEIYREQFWLRHTQALVEDFPGVGGIVGQTGWERLAEGYLSAFVPTSYTLRNL
                     GEKFADFVASQTDVEHHALVTDMARLEWAYVELFDAEDPPPLSLEELGKYGEEELGRA
                     RLRLSPGVRLLNVSFPVVELRKQILLAEGTAVPLPSPESCNLVVYRADLAILHERLDE
                     EAFTLLDALSRGESLMHACESACALGPEATAHVAEHVGEWLARWVRLKWIVAVAV"
BASE COUNT          738 a         1465 c         1448 g          735 t
ORIGIN      
        1 cggcgagggc atcgacgaag agacacgccg cgacgtcctg aaaatcgcga acgaaacgcc
       61 aggcgtgttg gcggtgaccc agcttctgag cttgcacctc gggccctcgt cggtgctgct
      121 cgcgctgaag gtgcgcttcc aggcgggggc gagcctgccc gacgtcgagc gcaccatcaa
      181 tcaagtcgag gagcgtattc gcgccgccga accacgcatg cgacgtatct tcgtggagcc
      241 cgacgccgag tacgacccct cacgcgaccc agacgctatc tagaacaggg ctgcgcgccg
      301 caccggcacc ggctcacgac ttcacgttga cgctcggcac cggttcccga cttcatgttt
      361 gacgctcggc accggttcac cacttcacgt tgacgctcgg cggctgaatc tcgacctcgg
      421 cgcgcgtgag ctccacgctg agcttcacga cggcggcgcc gtggcgctgc tctttcagat
      481 cgagccccgg gagcttgctc aagtcggcgc gcggcacgac ctgatccggt ttcaacgagt
      541 gcaccacctc aggtgggccg tagacgacga cgttgacggc gcggggcgtg ctggtgccgc
      601 tcgcgacccc cgcgatttcg acggggcggc tgtcgaagtg cacctctgcc aagcggcgcg
      661 cgatcgtgac ggtgacgttg accgaggtcg ggccgatgaa cttcacccgc ggaggcggcc
      721 cgtcgagtgc gatgcgtcgg cggtgcactc cctcgctcaa gccggatacg tcgaaaggtg
      781 ctagccgcgc ggactgaacc acctcgacga ggctgcgggg gccgcgcgca gcgagcgtcg
      841 ctgggtctgg ctcgggctct cccttcacga cgtagccgga agcaggctgc cccgaaatcg
      901 acgcttgcac tgggatctca cgcacgacga cgtcctccca gtcgagatcg atgctgggcg
      961 ggtcgatgat gctcaccttg agatcgggag gaagcttgaa ctcgcgcggg tcgaagacga
     1021 tttgctcttt attaccctgg cgcagatcga tttcgatggg gaggagccct tcctgcgcag
     1081 ctcgatcgag cgcgcgcgcc ggtccacgca gagtcaggtg aatgttcggc gggattgccg
     1141 tcatgagctc gcgatccgaa ccctccgccg gcgggcgcag gactaccgtc accggcaacg
     1201 tccgaagctg ctcgtcttgc ttgccgtaaa ggatcgcgaa caggccgact gcgatgctca
     1261 gcgagagcgc cttcaggccg atgttctcgg tgaacgattc cttcaaaaaa ccgaggaacc
     1321 aagagagaag cgcggtcact cgtcaccccg gcgcttcgac tgcgcgttgg gctctgcctc
     1381 ggccggctcg acacggggcg caactcgcgg tgcgaccggt gtgcgcagcg gcgtcgcggg
     1441 gctcggcagg ggaggttgcg gcgcgcgcag cggaggcggc ggcggttcgc ttcgcgggcg
     1501 caccggtgcg ggggtctcgg tcggcatgtc ggcgaccaag ctgatgcgcg ggtggtcgac
     1561 gtcttcgtcg acgtggcgcg gtgcgcgcgc gggttccggc ggacgcacgc tctccgcgct
     1621 gggctcccgg tgcgcgtcgc gctgagccgc caccttgcgg ctcttcttgc ggacgcgcgg
     1681 gctgaagatc gcctcgagca tggtcttgag cttcggcccg tcgaggttgc tcgcgatgtt
     1741 gccgttgaag caaaagctga tggtgcctcg ctcttcgctc acgatgacca cgaccgcgtc
     1801 ggtctcttcg gtgatgccga gcgccgcgcg gtggcgcgag ccgaagctgt cgtctacgac
     1861 gcggccttcg ggcatcggga agaagacgcc cgccttggcg atccgatagt tgcggatgat
     1921 cacggcgccg tcgtgcagct tgttcaccgc ctcgggaacg aagagcgaca ccaagagctc
     1981 ggccgtgacc tgcgcatcga gggtgtgccc tttgtgaact cccacgaact cgtcgaggtt
     2041 cgcgtcttgt tcgatggtga tgagcgcgcc gatgcggtgc cgcgcgaggt cggtcgcggc
     2101 ctcgacgacc tcgtcgatga cccgcgactc ttgccagcgc gcgagatgtc cgagccatgc
     2161 tcgactcccg acgcgctgca agccgcggcg aatgtcgttc tggaacacca ccaccacgat
     2221 cgtgatgagg ctcgcgacca ggtagctcat gatcgtgagc accgtcacga gctggagcca
     2281 cgttgcgacc aggtagatga aaaggacgac gcctaaccca agcgcgatct gcatcgcgcg
     2341 agtgccccgc acgacgagca gcgaacggta gatgagataa tagacgatga agacgtcgag
     2401 cccatcgcgc aggagctgga ggaaggtgcg ctcctcgaag tagctgcgga gcacctcaag
     2461 cacgaggcac ctcgcccgaa agacctcggt ccacgccacg cgccaccgcc agcgcttgac
     2521 gaacctcgag cacgtcgtgg acccgcacga ccgcggcgcc gcgtgcgacg caggcgagcg
     2581 acgcggcgat ggttccgccg agccgggcat cgggcggcgc cgggtcgacc gcgttgatga
     2641 acgactttcg actcggcccg acgactaccg gaacgccttc atcgagaaaa cgcccgaggc
     2701 ctcggatgag ctcgaacgag tgtcgcgcgt ttttggcgaa gccgatcccc gggtcgaacc
     2761 aaatcgactc gcgcgccaag ccctgctgca tggcgcgatc gcgcgcggcc cgccactcgg
     2821 cgagcacgtc ggccaccacg tcgccgtagg cgtcgtcggg gtagctcgag aagcctggca
     2881 tgttcgccat cggccctcgc gagtgcatca cgatcagggt cgccgagtgc cgtgcgcagg
     2941 ctcgcgccag ccgctcgtcg gcgagacacg agacgtcgtt gacgcaaacg gcgccgatcg
     3001 acaacgcgcg ctctgccacc gtcgggtcgg tcgtgtcgac ggaaacgaga gcgcggcgcg
     3061 acaccgcatg ccgcagcgcg ggctcgatgc gagcgagctg ctctcctgct ggaatcgcgg
     3121 gggcgccggg gcgcgtcgat tcgccgccga tgtcgatgat gtccgccccg gcctcgatta
     3181 gcgcatcgac ccgctcgcgc gcgctctcct cgtcgaggta gcgtccaccg tcgtagaagg
     3241 agtcgggcgt gacgttgagc acgcccatga gcgcagctcc gcgctctgcg cggcactgcg
     3301 aaaggcggat cgagagagcg cccacctcac ttcggtagca agcagttccg ccccgcgcaa
     3361 gcgaaactcg gcatcatttc ggctcgatcg tcgtcgttcg agaccgacga cgcgagcgac
     3421 gcgcggacct tctccgagag tgtcgaagcg agtttgcgcc tacaccgcga ccgcgacgat
     3481 ccacttgagt cgaacccagc gcgcaagcca ttcacccacg tgctcggcga cgtgcgccgt
     3541 ggcctcaggg cccagtgcgc acgcagattc gcacgcatgc atcagggatt ctccacggct
     3601 cagcgcatcc aagagagtga aggcttcttc gtcgagccgt tcatgcaaaa tcgcgagatc
     3661 ggcgcgatat acgacgaggt tacagctttc cggtgagggt aaggggaccg cggtgccctc
     3721 ggcgagcagg atttgcttgc ggagctcgac gaccgggaac gagacattca agagtcgcac
     3781 ccccggtgac aaccgcagtc gcgctcgacc gagctcttct tcgccgtatt tcccgagctc
     3841 ttcgagcgac aacggtggcg gatcttcggc gtcgaacagc tccacgtacg cccattcgag
     3901 gcgcgccatg tccgtgacca gtgcgtggtg ctcgacgtcg gtctggcttg ctacgaagtc
     3961 ggcaaatttt tcgcccaggt tgcgcaacgt gtagctcgtc ggcacgaagg cgctcaagta
     4021 cccctcggcg agccgctccc agcctgtttg tcccacaatt ccgcccacgc cggggaagtc
     4081 ttcgacgagg gcttgcgtgt gtcgcagcca gaattgttct cgatatattt cgagctgctc
     4141 gaccggcgac aagcggtcgt ttccagtcag gtaccgattc gcgagcgcga tcgcttcggg
     4201 atcgcgctcg agcgcgcctc gacgggtcaa catccgagcg agctgctcct cgagctcgtt
     4261 caacgacgcc aattcgccca cgcttcctcc ttcagcccac actggcctcg gccaggcttt
     4321 cccgcgggcc tccctggacc caaccgtgcc gctcctcggc ggccccgcgc cgcgcatctt
     4381 cgagtg
//
LOCUS       DASTKS010001161         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1077099, whole genome shotgun sequence.
ACCESSION   DASTKS010001161 DASTKS010000000
VERSION     DASTKS010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077099"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2664
                     /locus_tag="VFQ35_24690"
     CDS             <1..2664
                     /locus_tag="VFQ35_24690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9933934.1"
                     /translation="LSELARGFDRYADALTERAGSTFDPDIARELFVRLGRISEQRLE
                     DPNRAIAAYVRAVDQAGDQPELLEALDRLYSAAGDYEALAEILERRVVIETSDERRGE
                     LQYRLAVIQAREFKEPARALASLRAALDVAPTHEGAIEELEKLTELADLFDEAAEALE
                     NVYRAQNRAESLARLYEKRVAYADSPEARVDMRKNLALVLEQESQNPRAAQGVIEQGL
                     LDAPEDAGLLDEVERLAAITGDWRSAAEALERAVAAHPELSPESAVALCMRLSNWLSE
                     RASDVPGAERALNIALEHDPGNDEVLARLEQLQQGSGRERDLLQTLRRRARLQIDDEQ
                     RESLYRRAKDLADSLGDGQAAETVLRELLALDDTNLWALESLTLLRERAGDYPETFAL
                     VVRQSEIRSDAEGVRALRRRAAELSRDKLGDARRAVEFFEQLFEEDPADQASAQALEV
                     LYPQLGSFQELSRLYERQIEVATTPAARSALRLELARLNQDKFGAVESAIENLRAVLE
                     EEPAHELAVSRLGALLEAGGRDEELAELLNGQIEQAQSRGDVASELGFQVRLGEIYEQ
                     RLGDTARAIDTYKSVLQRDPQHHGALEALSRLLRKDGDLAECARVLSQLLNMESGEAA
                     VKRSLELADVFDALDQPSDAAEALERGLATGPRSPDLYRRLQAAYEKLGRWDKLSDLL
                     ARKASEEASPDEAVRLLREASQITARKRSDPVHAAELLERAAQLRPDNRELLLELCDR
                     YSAAGRGREAARVLEKIVANQGPKRTKELGEIHRRLAAAYLGEGEIHKAVEELDKAFR
                     IEPGNLQVLTQLGEVAMKVGDYKKAQQMYRALLLQKLDDSSAIKKSVVFLRLGEIHEA
                     LGEKPKAAQMYERAVQTDGLDEAKAKLKALQ"
     gene            2959..3834
                     /locus_tag="VFQ35_24695"
     CDS             2959..3834
                     /locus_tag="VFQ35_24695"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9933935.1"
                     /translation="MSQDPLPAARSTLPYDPRIDYQPRSGFLGGVGRKAIHVQAELGS
                     LFSTLGQAISAWFAPGRSARRTVHGVLLAQILFTGVQALALVSVIGILIGATTVVQTS
                     LMVPGADAELLGKILVAVVLRELAPLITAIIVAGRSGTAIATELGNMKVNYEVLGLES
                     LGIDPPRFIVLPRVLAAIVSVVVLMVYFGVVSVLSAALVGQEVLGTNPAELRTGLAQA
                     LLPHDIILFLAKGVGLGAVLGWFACHYGLRVRTSPTEVPRQASTAVVRSLLGCVLFDT
                     ALTVLFYWLSERPLH"
     gene            3868..4566
                     /locus_tag="VFQ35_24700"
     CDS             3868..4566
                     /locus_tag="VFQ35_24700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9933936.1"
                     /translation="MTNLLSDFGLDLDRVSFRYGELWVLRETSLSIPRGGNLLVTGEN
                     GVGKSTLMYVAAGLYASATGRVLLAGQAVQRARPSEIFRRGVRRGFVFHSGGLLSNTT
                     ALSNVMLPLRYHADLLGLDEQQVDRRARELLAEMRVSQGDYHALPAHLSAGVRRRVSI
                     ARALAIQPNFLFFDDPDANLDSATRRLVYELLERFRDDHQVTLLVTTTNRELTERLGL
                     PARELAHGYLLDRL"
BASE COUNT          807 a         1442 c         1634 g          838 t
ORIGIN      
        1 ttgtcggagc tcgcacgtgg gttcgaccgc tacgcggacg cgctcaccga gcgcgcggga
       61 tcgacgttcg acccggatat cgcacgcgag ctgttcgtgc gcctgggtcg gatcagtgag
      121 cagcggctcg aagatccgaa ccgcgcgatt gcagcctacg tccgcgccgt agatcaggcc
      181 ggtgaccagc ccgagctctt ggaggctttg gataggttgt actcggcggc gggagattac
      241 gaggcgttgg ccgagatcct cgagcgacgc gtcgtaatcg agacgtcgga cgaacggcgc
      301 ggagagctcc aataccgact ggccgtgatc caggcgcgtg agttcaaaga gcccgcgcgc
      361 gcgctggcct ccttgcgtgc ggcgctggac gtagcaccca ctcacgaggg cgcgatcgaa
      421 gagctggaga agctgacgga gctcgcggat ttgttcgatg aagccgccga ggctttggag
      481 aacgtttacc gcgcgcaaaa ccgcgccgag agcctcgcgc gattgtacga gaagcgcgtc
      541 gcctacgcgg actcgcccga agcgcgcgtc gacatgcgca agaacctggc gttggtgctg
      601 gagcaggaga gccagaaccc gcgcgcggct cagggggtga tcgagcaagg cttgctcgac
      661 gcaccagaag atgcggggtt gctcgacgaa gtcgagcgct tggcggcgat cacgggtgac
      721 tggaggagcg cggccgaggc actcgaacgc gccgttgcgg cccatccgga gctctcgccg
      781 gagagcgcgg tagcgctctg catgcggctt tcgaactggc tctcggagcg agcttccgac
      841 gtgccgggcg ccgagcgagc cctcaacatc gcgctcgaac acgatccggg taacgacgag
      901 gtgttggctc gtctcgaaca attgcaacaa ggctcgggcc gcgagcgcga tttgctccag
      961 acattgcggc gccgtgcgcg cctgcagatc gacgacgagc agcgtgaatc cctgtatcgc
     1021 agggccaagg acctcgccga ttcgctcggt gacggtcaag ccgcggagac ggtgctgcgt
     1081 gagctcctcg cgctggatga caccaacctg tgggcgctag aatcgttgac tctgctacgc
     1141 gagagagcgg gggactatcc cgagacgttc gccctggtcg tgcgccagag cgaaattcgc
     1201 tccgacgccg aaggggtgag ggcgctgcga cgccgagcgg ccgagctgtc gcgcgacaag
     1261 ctcggtgacg cgcgccgcgc cgtggagttc ttcgaacaat tgttcgaaga agacccggcc
     1321 gatcaagcct cggcgcaggc cttggaggtg ctgtatccgc agctcgggtc gttccaggag
     1381 ctatcacggc tctacgagcg gcagatcgag gtcgcgacga caccggcggc tcgcagcgct
     1441 ctgcggctcg aactcgcgcg cttgaatcag gacaaattcg gggcggtcga gtcggcgatc
     1501 gagaacttgc gcgcggtgct cgaggaagag cctgcgcacg agctcgcggt ctcgcggctc
     1561 ggcgcgttgc tcgaggcggg cggtcgcgac gaggagctgg cggagctctt gaacggccaa
     1621 atcgagcaag cgcagtcgcg cggcgacgtt gcttccgagc tcggcttcca agttcgcttg
     1681 ggcgaaatct acgagcaaag gctgggcgat acggcgcggg cgatcgacac ctacaagagc
     1741 gtgctgcaac gcgatccgca gcaccacggc gcgctcgagg cgctgtcgcg gctgttgcgt
     1801 aaagacggcg atctggcgga gtgcgcgcgt gtactcagcc agctgctcaa catggagagc
     1861 ggggaggccg ccgtgaagcg ctcgctcgag ctcgcggacg tgttcgacgc cctggaccaa
     1921 ccgtcggacg cggccgaagc attggaacgt gggctcgcga ccgggccacg cagcccggat
     1981 ttgtaccgtc ggctccaggc cgcttacgag aagctcgggc gttgggacaa gctttccgat
     2041 ctcctggctc gcaaggcctc ggaagaagcg tcaccagacg aggcggtgcg gttgttgcgc
     2101 gaagcctcgc agatcacggc gcgtaagcgc tcggatccgg tgcacgcggc ggagctcctg
     2161 gaacgtgccg cccagctgcg accggacaat cgcgaactct tgctggagct ctgtgatcgt
     2221 tacagcgcgg cgggtcgtgg acgcgaagcg gctcgcgtgc tcgagaagat cgtggccaat
     2281 caaggtccga aacggaccaa ggagctcggc gaaattcatc gtcgtctcgc cgctgcgtat
     2341 ctgggggaag gcgagattca caaggcggtg gaggagctcg acaaggcttt ccgcatcgag
     2401 ccgggtaact tgcaagtcct cacgcagctc ggtgaagtcg ccatgaaagt gggcgactac
     2461 aagaaggccc agcagatgta tcgcgcgctg ttgctgcaga agctcgatga ctcttcggcg
     2521 atcaagaaga gcgtcgtgtt cctgaggctc ggtgagattc acgaagcgct cggagaaaag
     2581 ccgaaggccg ctcaaatgta cgagcgcgcc gtgcagacgg atgggctcga cgaagccaag
     2641 gccaagctca aagcgctaca gtgaatctgc tcgtgcaaga cccggggcgc gttctttcgg
     2701 gcgcggcccg gggcgggcat gcgccgatcc gggtggccgc gggccgcgtt cgcctcggcg
     2761 cgagtgcatc caggtccggt gaggtggggg atagacgccg agcaggacga tcgagctcga
     2821 ccacgagcta gacgggcaaa attcggggca aatcggccat ttgcctggcc cggggtgaag
     2881 ctcggatgcg ggctggccgt agctgggtgt gggccctctc cagtgacggt cggggctcga
     2941 ggttcgagtg ccatgtcgat gagccaagat ccccttccgg cagcgcgatc gacgctcccg
     3001 tatgatccgc gcatcgacta ccagccgcgt tctgggttcc tgggcggggt gggacgcaag
     3061 gcgattcacg ttcaggccga gctcggctcg ttattctcga ccctaggtca ggcgatttcc
     3121 gcctggttcg cgcccggtag gtcagctcgc cgcaccgtgc atggcgtgct cctcgcgcag
     3181 atcctcttca cgggtgtgca ggcgctagcg ctcgtcagcg tgatcgggat tttgatcggc
     3241 gctaccaccg tagttcaaac cagcttgatg gtgcccggcg ccgacgccga gctgctcggc
     3301 aagattttgg tggcggtggt gctgcgtgag cttgcgccat tgatcaccgc catcatcgtg
     3361 gcgggccgct cgggtaccgc aatcgcgacc gagctcggca acatgaaggt gaactacgag
     3421 gtgctggggc tcgaaagctt ggggatcgat ccgccgcgct tcatcgtgtt gcctcgcgtg
     3481 ctcgccgcca tcgttagcgt cgtggtgctg atggtgtact tcggagtcgt gtcggtcctt
     3541 tctgcggcgc tggttgggca agaggtgttg ggtaccaacc cggccgagct ccgcaccggc
     3601 ttggctcagg ctttactgcc ccacgacatc atcttgttcc tggccaaggg agtgggcttg
     3661 ggtgccgttc tcggttggtt cgcgtgtcac tatggcctac gcgtgcgaac gtcccccacg
     3721 gaagtgccgc gccaggccag cacggccgta gttcgttcgc tgctcggctg cgtgttgttc
     3781 gacacggcgt tgacggtgct gttctattgg ctctcggaac ggcccctgca ttgaaaccag
     3841 gctgtgcccc atgacgggtc tgcgcccatg accaacctgc tcagcgactt cgggctcgat
     3901 ctcgaccggg tgagctttcg ctacggcgag ctctgggtgt tgcgcgagac tagcctcagt
     3961 attccacgag gcggcaacct gctggtgacg ggtgagaacg gcgtcggcaa gtccacgctc
     4021 atgtatgtgg cggccggcct ctacgcgagt gcaactgggc gcgtgctctt ggcgggtcag
     4081 gcggtgcagc gcgcccggcc gagcgagatc tttcggcgcg gggtgcggcg cggcttcgtg
     4141 tttcacagcg gcggcctgct ctccaacacc accgccttga gcaacgtgat gctgccgctg
     4201 cgatatcatg cggatttgct cggtctcgac gagcagcagg tggaccggcg cgcgcgcgaa
     4261 ctactggcgg agatgcgcgt ttcccaggga gactatcatg ctctgccggc gcacctctcg
     4321 gccggggtac gccgccgcgt cagtatcgcg cgcgctttgg cgattcagcc caattttcta
     4381 ttcttcgacg accccgacgc aaacctagac tctgcgacgc gccgcttggt ctacgagctt
     4441 ctagagcgct ttcgggacga ccaccaggtc accctgctgg tgaccacgac caaccgcgaa
     4501 ctgaccgagc ggctcgggct gcccgcccgt gagctcgccc acggatacct gctcgatcgc
     4561 ctgtgagtgc tcgtgtttga cggtagccgg gcgcgcgcgg ctcgaaatct gagctagtac
     4621 tgccgcgtga cgccagccgg gcaacaaccg agtgccttcc aatacgtcga ggattgggcg
     4681 cgcgaataca ttcgctcgac cagcctgagc tacaagatcg c
//
LOCUS       DASTKS010001162         5481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1972578, whole genome shotgun sequence.
ACCESSION   DASTKS010001162 DASTKS010000000
VERSION     DASTKS010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972578"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFQ35_24705"
     CDS             <1..409
                     /locus_tag="VFQ35_24705"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HET9933937.1"
                     /translation="NGYKTILTDTQANLFEAVQVYSVKGTGEYLMIVEAMGSGGRYFR
                     AFRASSLGGTFTPIPGASSESTPFAGKKNVTFSGTAWTNDISHGDLIRSDTSETQPID
                     PCNIQFLYQGRNPSSNVDYSELPYRPGLLTKIN"
     gene            complement(447..1286)
                     /locus_tag="VFQ35_24710"
     CDS             complement(447..1286)
                     /locus_tag="VFQ35_24710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933938.1"
                     /translation="MTGSKGSLGLVVLMMSAKGGCSTPVSIGTDYAGGAAGAAAGGRT
                     SVGGQDVRPPSSGGANGGSNGGSVAVGGAVPSGGRADGVGGNVGGAGGGDQAGSTASG
                     AAGRDGAGGSTSGGLGGIAGAAGAPAGGAADAGATGSSGAGGGAIGTCPTALPLKCGD
                     RLNHSTVTEGRASQWNGYNLSARAENGRETLYAFQTANRCRVVANLKNLTTDLDLFLL
                     SRCEQESNVRSNEAASSTPLDLQTVESVSWTNSAGAIHYVVVDGYAGDEGSYTLEVDC
                     TCD"
     gene            complement(1376..2188)
                     /locus_tag="VFQ35_24715"
     CDS             complement(1376..2188)
                     /locus_tag="VFQ35_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933939.1"
                     /translation="MTETTDIDDGLAALYRVARTQEAPTHADRLAVRAAVASTLATSA
                     SLASTQVVASSVVAAPATKAAITAKVATWLCAGIALGGVSSSVAWVLSEPPDVVIQHS
                     AALPPGSASASNRAYGRRSPLPSESVAGSRGASVLDSPGESSARLPTSAPLSIPSARP
                     RATPNRSREVTETRALRSGVEPSSLLAESEGVQRVQQALANGDASRAIQLLAEQDSRF
                     RDGALAEERAALGVLALCAKGQSEAAEAARQRFLVTYPRSPHEVRLRNSCTK"
     gene            complement(2185..2763)
                     /locus_tag="VFQ35_24720"
     CDS             complement(2185..2763)
                     /locus_tag="VFQ35_24720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9933940.1"
                     /translation="MTGLVDSARKVSVLEPKTPTENTVEAIYAGHFDFVWRNLRRLGV
                     DEEQLRDAAQDVFVVVHRRIGEWDGSETLRPWLYSIVRRVAADTRRRRRRKEPLARED
                     AQALVDVRERGPESNASRKQELALLLALLEQLDEEKRAVLILIDLEGVSAPQAARALN
                     LNLNTVYSRLRAARLEMRKAYALATGREGNQP"
     gene            complement(2809..3756)
                     /locus_tag="VFQ35_24725"
     CDS             complement(2809..3756)
                     /locus_tag="VFQ35_24725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933941.1"
                     /translation="MQLEAPIECPSAAEVVAAVDALVQTTPAQPLEVGAQIQHDAEGF
                     SLKVTLGAGERRVHGDTCEAVTQALVAIVALAIDPSVQLSPPSPEVGQARGEVVGEGG
                     EVATQGSSTPTAALSATAVARDPLPTRQRPADTGRASGSTDVSRAPPAPRFGASLLGF
                     GEQGLLPSPSLGVGALLRATLAPWSLELGGSWLRPRWAQVEQVAARKGGYISWVSAQA
                     NGCRALGPSAAACAGLELGQVGGEGEGVSNGTAGHALWLAGALSALGRLRLGAGFSLE
                     GRVGAAFPLYRPEFSIEPYGALHRASWLSGRVSAGIGFH"
     gene            4049..>5481
                     /locus_tag="VFQ35_24730"
     CDS             4049..>5481
                     /locus_tag="VFQ35_24730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933942.1"
                     /translation="MAILLTFVFCQVVAAVASAQDANGGAPATSALAAGAPASSTPPG
                     GQKEVPEGRVEVAGTSGADSAAPKELQARIDSDDFEYRIAIAEVERRPVKTVEILADP
                     LLDEKGKQRLFDLGVEGAPMSHDSVTIAKPLGAGDRRTLIVRARLPELGTYTMTLTPY
                     WGGAPRQKIVLKIVRERPTLGLEVADATTFAQSGDTDTVRFSLNLRETAGRSVTLDAP
                     ALVSLVQQMGPDSKAAAINRYAKYAFGLDDKKFDEPQAIPFTKGQSRTLAFRLSDFPG
                     PGKYEAKLQFGFKDATPISKAVTLYVRENWTLAAIAIGLGVLVSYSLRRWVSREQPRL
                     ELLKEATRLAPEVDAALRTKGIDATEKGLVIQVGGLLTELLSRISQGRKGADTAAIDV
                     MGIRIAATRSFLVVRALVRQQPANVATEFLTKLEAARAVLTDPNADLTALKAQESAIR
                     AIPDQVAAKRNELAAAGAQMGGGDEARR"
BASE COUNT         1001 a         1852 c         1718 g          910 t
ORIGIN      
        1 caacggctac aagacgatct tgaccgatac ccaggcgaac ctgttcgaag ccgtgcaggt
       61 gtattcggtg aagggaacgg gcgagtatct gatgattgtc gaggcgatgg gcagcggcgg
      121 gcgctacttc cgcgcgttcc gggcttcgag cctcgggggc acgtttacgc ccatccccgg
      181 agcgtcgagt gagtcgacgc cgttcgccgg caagaagaac gtcacgttca gcgggactgc
      241 gtggaccaac gacatcagcc acggcgatct gatccggagc gacacgtcgg agacgcaacc
      301 gatcgatccc tgcaacatcc agtttctata tcaaggcaga aacccttcca gcaacgtcga
      361 ctatagcgag ctcccgtacc ggcccggctt gctcaccaag atcaattagc agaagcggac
      421 gctacaaacg tgaatccgac cacgactcag tcgcaggtgc agtcgacttc cagcgtgtag
      481 ctaccctcgt ctcccgcata gccgtccacc accacgtaat ggatggcgcc cgccgagttt
      541 gtccaactga cgctttccac ggtttgaaga tcgagaggag tcgaagacgc cgcttcattc
      601 gacctcacgt tcgattcctg ttcacagcgc gagagcagga agagatcgag atccgtggtc
      661 aggttcttca ggttcgcgac gacgcggcag cggttcgcgg tctggaaagc gtaaagcgtt
      721 tcgcgcccat tttcggcgcg cgcgctcagg ttgtacccat tccactgact ggctcggcct
      781 tccgtcaccg tgctgtgatt caaacgatcc ccacacttga gcggcaaagc cgtgggacat
      841 gttccgatgg ccccgccgcc ggcgcctgat gagcctgttg cgcccgcgtc cgccgcaccg
      901 ccagcaggcg cgcccgcggc cccggcgatg ccgcccagtc ctccgctagt acttcctccc
      961 gccccgtctc gccccgccgc gccggaagcg gtgctccccg cctggtcacc gccgcctgct
     1021 ccgccgacat tgcctcccac tccgtcggcg cgtccgcctg aagggacggc gccgccaacc
     1081 gcaaccgaac caccgttgct gccgccattg gcgccgccgc tcgagggcgg acggacatcc
     1141 tggccgccga ccgacgtacg gccgcccgct gccgccccgg cggcaccacc cgcgtagtca
     1201 gtgccgatcg aaactggcgt cgagcacccc cccttcgcac tcatcatgag caccacgagc
     1261 ccgagcgaac ctttggatcc tgtcatgttc attgagctcc gatcgatgcc gtttgcgcgg
     1321 ttccactttc gagcgccgct actcaccgcc atggccggga ggcggtgttc cgtcatcatt
     1381 tcgtgcagct gttgcgcagc cgcacttcgt gaggtgaccg cgggtaggtg acgagaaagc
     1441 gctgccgtgc ggcttctgcc gcctcgcttt gacctttggc gcacaaggcc agcacgccga
     1501 gcgcggcgcg ctcttcggcg agtgccccgt cccgaaatcg cgagtcttgc tcggcgagga
     1561 gctgtatggc gcggctcgcg tcgccgttcg cgagcgcttg ctggacgcgc tgtactcctt
     1621 cgctttcggc gagaagcgaa gacggctcta cgccggagcg gagggcacgg gtctcagtca
     1681 cctcacggga gcggttcggc gtagcgcgtg gtctcgcgga aggtatggag aggggtgcag
     1741 aggttggcag cctcgccgaa gattcacctg gcgagtccag taccgaagcg cctcgggaac
     1801 cggcgacgct ctccgatggc agtggagagc gtcggccgta agctctgttc gaagccgaag
     1861 ccgaccccgg cggcaacgcc gcgctgtgct ggatcacgac gtcaggcggc tcgctgagca
     1921 cccaagccac cgacgaagaa acgccgccca gcgcgatgcc cgcgcacagc caagtcgcga
     1981 ccttggctgt aatggccgcc ttggttgcag gcgccgcgac gacgctcgaa gccaccacct
     2041 gggtcgaagc caacgaagcg ctcgtcgcca acgtgctcgc gacggcggct cgtactgcaa
     2101 gccgatcggc gtgcgtcggc gcctcttgcg tgcgcgcaac ccgatacaga gcggcaagcc
     2161 cgtcgtcgat gtcggtggtc tcggtcatgg ctgattccct tcccggcccg tggcgagcgc
     2221 ataggctttt cgcatttcga ggcgcgcggc gcgcagacgg gaatagacgg tgttgagatt
     2281 gaggttcagc gcgcgagccg cttggggcgc actgacccct tccaagtcga ttagaatcaa
     2341 taccgcgcgc ttctcttcat cgagctgctc gagcagagct agcaacaggg ccagctcttg
     2401 cttgcgggat gcattgcttt ctggcccacg ctcgcgcaca tctacgagcg cctgagcgtc
     2461 ttcgcgcgcg aggggctctt tgcgtcgccg tcgacgccgt gtgtcggccg caacgcgacg
     2521 cacgatcgag tacagccaag ggcgcaacgt ctcggagccg tcccactcgc cgatgcggcg
     2581 gtgtacgacc acgaatacgt cctgcgcggc gtcgcgcagc tgctcttcat ccacgccgag
     2641 acgccgcagg ttgcgccaca cgaaatcgaa atgaccagca tagatcgctt cgaccgtgtt
     2701 ctcggtgggg gtcttcggct cgagcaccga gacctttctc gcggaatcga ccaagccagt
     2761 catcgaggag gctgtcgtgg gcaatggcca ggcggcgcat ttccgtgatc agtgaaagcc
     2821 gattcccgcg gacactcgcc ccgacaacca gctcgcccga tgcagcgcac catagggttc
     2881 tatcgaaaat tccggacgat acagcgggaa agccgcaccc actcggccct cgagggagaa
     2941 gcccgcgccg agccgcagac gccccagcgc ggagagcgcg cccgcgagcc acagcgcatg
     3001 accagccgtg ccgttcgaaa ccccttcgcc ttcaccgccg acttgcccga gttctagccc
     3061 tgcgcacgcc gctgccgatg ggccaagcgc gcggcagccg ttggcttggg cgctcaccca
     3121 agaaatgtac cctccctttc gtgcggccac ctgttcgacc tgtgcccagc gcggccgtag
     3181 ccaactgccc ccaagctcta ggctccaggg cgcgagcgtc gctcgtagca gcgcgccgac
     3241 cccgagggac ggcgacggca gcaaaccctg ctcaccgaag ccgagcaacg acgcgccgaa
     3301 ccgcggcgct ggcggcgctc tcgacacatc cgtcgagccc gacgctcggc ccgtatctgc
     3361 gggacgttgg cgagtcggca gcgggtcgcg agcgaccgcg gttgcagaga gagccgcggt
     3421 cggggtcgaa gagccttgcg tagcgacctc tccaccttcc ccaaccacct cgcctcgagc
     3481 ttgaccgact tcaggcgagg ggggcgaaag ctgaaccgat gggtcgatcg ccagcgcaac
     3541 gatcgcaacc aacgcttgtg tgacggcttc acacgtatcg ccatgaaccc ggcgctcgcc
     3601 ggcgccgagc gtgaccttca acgaaaagcc ctctgcgtcg tgctgaattt gcgctcctac
     3661 ctcgagtggt tgcgcgggcg tcgtctgcac gagcgcgtcc actgctgcca ccacctcggc
     3721 cgcgctcgga cactcgatag gtgcttccag ctgcaagtcg agcgaggacg aaccagaatg
     3781 agacctcggc tcgctcgctg ccagaagcag cgacgaaagc cccgccgccg cgagaacagg
     3841 cgcgaggcgc cgtgctcgct cgtcgaacca agacctccag gagaccatcg agcgacgagc
     3901 atcagccgca ggccgagcga cggcaagagc cgtttcagcg tcgtctacat ggccacctct
     3961 cctccaggct tcgcccggcg cgaataaaat gactagacac cttggttgag cgcatccgag
     4021 tcccacgact actattagag ggatgagatt ggctatcttg ctgacgttcg tcttctgtca
     4081 ggtcgtcgcg gcggtcgcga gcgcacaaga cgctaacggc ggagctcccg caaccagcgc
     4141 ccttgcggcg ggcgctcctg catcgagcac tccgcctggc ggccagaagg aagtccccga
     4201 agggcgcgtc gaggtagcgg gaacctcggg tgccgactcc gccgctccca aggagcttca
     4261 agcccgcatc gacagcgacg acttcgaata tcgaatcgcg atcgccgagg tcgaacgccg
     4321 cccggtcaaa accgtcgaga ttctcgccga ccccttgctc gacgaaaagg gcaagcagag
     4381 gctcttcgat ctcggcgtcg agggcgctcc gatgtctcac gactcggtga cgatagcgaa
     4441 gccgctcgga gccggtgacc gccggacact catcgttcgc gcgcggctgc ccgagctcgg
     4501 cacctatacg atgacgctca caccttattg gggtggtgct cctcgacaga agatcgtgct
     4561 gaagatcgtc cgtgagcgtc cgactttggg tctcgaagtc gccgacgcca cgacgttcgc
     4621 ccaaagcgga gacacggaca cggtgcgttt ttcgctgaac ttgcgggaga cagcgggacg
     4681 tagcgtgacg ctcgacgcgc cggcgctggt ttcgttggtg cagcaaatgg gccccgactc
     4741 gaaagcggcc gccatcaacc gctacgccaa gtacgcgttc ggactggacg acaagaagtt
     4801 cgacgagccc caggccatac ctttcaccaa agggcaatcg cgaacgctcg cgtttcggtt
     4861 gagtgacttt ccgggtcccg gaaagtacga ggccaagctg cagttcggct tcaaggacgc
     4921 gactccgatc agcaaggcgg tgacgctcta cgttcgggag aactggaccc tcgcggcgat
     4981 cgcgattggc ttgggagttc tggtttcgta ctcgttgcga cgttgggtgt ctcgggagca
     5041 gccacgactc gagttgttga aagaggcgac acggctcgcg cccgaggtcg atgccgcgct
     5101 ccgaacgaag ggcatcgacg ccacggagaa gggcctcgtg atccaagtgg gaggtcttct
     5161 gaccgagctc ctctcacgca tttcccaggg aaggaagggc gccgacacag ccgcgatcga
     5221 cgtgatgggg atccgcatcg ccgcgacccg gagcttcttg gtcgtccgag cgctcgttcg
     5281 ccaacaaccg gccaacgtcg cgacggagtt cttgacgaag ctcgaggccg cacgcgcggt
     5341 cctgaccgac ccgaacgcag atttgacggc gctcaaggca caagaatctg cgattcgcgc
     5401 catcccggat caggtcgcgg cgaaacgaaa cgagctcgcg gccgcggggg ctcaaatggg
     5461 cggaggcgac gaggctcgac g
//
LOCUS       DASTKS010001163         9315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_629956, whole genome shotgun sequence.
ACCESSION   DASTKS010001163 DASTKS010000000
VERSION     DASTKS010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629956"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFQ35_24735"
     CDS             <1..442
                     /locus_tag="VFQ35_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933943.1"
                     /translation="VAPGSERAVTSAARLESALVRLAAARRERTASTKATPTAARGLP
                     LGSEGSSGGVAPSLGGGLQALAARFGTPESDGARPMPTSESSRQDPSARRAATGFEDG
                     AEALPPLPRPLLERLSRNELTRRLDEILRHEAERSGVDFNRRMP"
     gene            439..1398
                     /locus_tag="VFQ35_24740"
     CDS             439..1398
                     /locus_tag="VFQ35_24740"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HET9933944.1"
                     /translation="MTLSRPRARVTLDGQALSSAEAALARVEVSLWLGARHDHAYVAL
                     WPDSRFADVAPGATLSIALGEDGAEEGVFSGTATRVTAGADGIEIDGLSLSAKLSKFR
                     RSQTYVEQTVADIVRDLAGSVSVDSVAGDLKLASYSVDQRRSAWDHIVELATLTGADV
                     ASTASGALRFVRPRTGTPDVTLRYGADVLAWEIGPVVEPGVPKVAAHGAGSEAGSEQW
                     HWVLASPPAQGNGDGPVDVVPAFTTRDAAEQLARARAEAGKRAAVSARLTTLGRHDLR
                     PGSLVRVSDLPTGDAGVLRVLGVEHCLDSRTGFVTNVTAEGAG"
     gene            1395..2069
                     /locus_tag="VFQ35_24745"
     CDS             1395..2069
                     /locus_tag="VFQ35_24745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933945.1"
                     /translation="MSDIIGTLRAIIRDELARQRHAELGVVTRVHAKDGDDSKNNHQV
                     DLKLRGSDVELRRVPVAVGRLGVSALPNEGDLMIVVFVGGDLNGAVAVGCVYDQERHP
                     PVAALHELVYQPPDPEASGVKRLHLELQNGSTVTLDDESLQVSLGDTSLVINKDGDLS
                     IEAAGDLKFKAGGKVTIEASGDVTITAQGNLNAKGTSASLEGQSEAKVKGATLTIAGN
                     TQFSPS"
     gene            2078..2410
                     /locus_tag="VFQ35_24750"
     CDS             2078..2410
                     /locus_tag="VFQ35_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933946.1"
                     /translation="MPGPPVSVGCSVMLSPGASGPPDTGVVTTILQTNVVVGGLPLAT
                     TGSLCQMVNSVSGAPYVLPIGQGGSSGVGISNQALVRVGDRIPSGPGILTILGPPAAP
                     FINDTNSP"
     gene            2453..2938
                     /locus_tag="VFQ35_24755"
     CDS             2453..2938
                     /locus_tag="VFQ35_24755"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET9933947.1"
                     /translation="MNNPRSLDKIAPLFGKDLALDYAQSGGFFEDADLRSGRRPNAPG
                     IRRDLSVASGIENITQAIANRLKTRRGELAALGHPEYGSRHHELVGEPNVERTRNLIK
                     LYVLQALRQEPRIEKVLKATVRAEHEPPREVVRVELEVRVIGEPVPLNLVVPFSLEVT
                     L"
     gene            2935..4749
                     /locus_tag="VFQ35_24760"
     CDS             2935..4749
                     /locus_tag="VFQ35_24760"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HET9933948.1"
                     /translation="MSYAAEPYSQFVDDLLTSLTGGVAREQFVFRAEEGSFRLSPPGP
                     IVPSSLSVFGQSAGAFQRFQPDRDFVLAPDSSLSWQTAADGGPAADAIWPDEGTPFFA
                     NYDHTGPLGSAPLLSDRNPGSVVRLLAESFGREFAVVSKQLEAIHRAGFLSTASGRDL
                     DQLVALVGLTRRTRAFATGSVIFSSAAPARADITIQAGTRLSTAEPPAITFETTEDRT
                     LHRGELTVEAPIQALVSGGAGVVPARAVSVIHRPIFGIESASNPQATEQSGDDESDAA
                     LRARARRALETAGGATTGALLGALASLPGVRDNDIRVAEDHLARPGVVTVNVAAPLDG
                     AAALRAILLIDEKRPAGVRVLTNLDAPAPPGGPSAPLNVVDDADAEDTGELFVDGLYL
                     PVQVRIVLLAAAATLSANERSALTTAAAGVARRFVAEAGIGEVLVYNRLVAGIMALEG
                     VLDVALELSPKVLPGEPVGPRHQNLIPPETLRPRLSDTDLSVEIAGELVSFDVTVTVA
                     LTPLGQSVDPSVSLEVARLQIAAQLQDKITAIVGPITATGLRDKVVPTEYFSLSALHY
                     RVQYLEAGLSINVDDPQIQLAELERAWIRTVRLSAESG"
     gene            4752..7496
                     /locus_tag="VFQ35_24765"
     CDS             4752..7496
                     /locus_tag="VFQ35_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933949.1"
                     /translation="MTGRAFQVLSRLPAHLEPTRPGKQLSAVVEALVVDLERLSADLA
                     GVRRSHRLAHADTLRDVLLLGGMHGLARPDVELVIARIEATRSIARALEAAVVAKATA
                     ERDELAERLFTLWGIQGGSPRLADLAPPFAPGETPNLDAAARALIESVRMSFGFSALL
                     DGVRRRVERVARLHAEGNGTVYALIEAAASVLDLELDVEANQRVKAASRPVVRASTGG
                     TPGTATWSYVVVARPLVRNVARNSRLAVVVRGPAALSETDFVKLEWSSVPDARDYLVY
                     RVRSGGEPSTVGLLTPTPLESTTTTFRDTGVPAVAPATPLPETDDGLFHSADRFWHSS
                     FVRDRIRPMRSVEPALPPQFVALDSTIVLDDFATALGQAPAAVLERAGALAAAGALLD
                     GEVHPDLALRVANRLGSALVFRRPISVRALASALAAPRADVFRALAALLSEGANARIS
                     IELSTADPLAASFGFLVVQSRRLFVDDGVRLGELERRTGVELDQLVSELVKLDVPGAG
                     AETRLTFAEAARLLPRVGFRAVRPRIFLLDARAPISVAELSVRAALPASRLLRDLALL
                     GVAAPTPGTLLDADRVAQLLRPHRFAVREILAASDDLVGIEENPMRDEETASTPRVHT
                     ELFPVFRRGFGRVRLEARIVGIETRTVGPMLVNRDEGHGIGLARAVPQGKMLVFSADG
                     RARLTSVEPAEGESDEDVTGFAYSWQGACFAGTDDDPANPRDFLFDGPDVPENRRATF
                     AVAAPAGALDPEFPFPHAGAPIAMPGVNVGQTRFAFFVQEAHFAGLDESVAPPAAIPP
                     VPRPKVGFADGAVFGVGSGEERAPAAEVSLAWREHEAYAVRVLIPKRFSALDTEGAPT
                     VAERVRVALERVRPVGVEVRVEYQDDRWILGEAAVSADRTDPILALMGGTALWPPPTG
                     "
     gene            7525..8220
                     /locus_tag="VFQ35_24770"
     CDS             7525..8220
                     /locus_tag="VFQ35_24770"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9933950.1"
                     /translation="MLNFSKTHVNPGEPVTARAWNDIVDGLFEVQALLKAAGGTATVQ
                     VTGAGVDSRRVRVTATSADGTPAEALRPIPPSTDFIFPRLAAGAYQIRIEAPGFDVAT
                     GAVTVGEDGSVSPKPLAIALTANRVVMPSVFGLKLPEAVALLGSMQLRILDVSGKSLP
                     KTGFESTYNNAPVLVQSPSPGELVQAEGAFLVVAAGLVVEPLVVVPNLQGKTYAQARA
                     ALETLGLKINVIK"
     gene            8231..8635
                     /locus_tag="VFQ35_24775"
     CDS             8231..8635
                     /locus_tag="VFQ35_24775"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9933951.1"
                     /translation="MATKIKASYSTEVQDKLSRYDTVKAEAEVVFQSVAPGTTIRRGE
                     TVNVRLISSSDVPLHDLVKEGLPERFTKLTLADLDDVGGDPEIIGIIKDPAKLKDPTE
                     RIKAKNSLEKSLNTNLSEQEADAMFGIVGAVF"
     gene            8650..>9315
                     /locus_tag="VFQ35_24780"
     CDS             8650..>9315
                     /locus_tag="VFQ35_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933952.1"
                     /translation="MAQASSATLVGNLDAIQEASEFALFEQNLVYETLHAPLVRTDGI
                     SSRTVVKRLHRNALVRRLNYVVSAGRAAAALLANAGQVRLQESTPGLTQVVVDFGVPR
                     TVASVGALDATGGPELKILSVSVWDGAKFLKTAHYSASWAQAYQNGTVVDIVTASDPS
                     TAKMAIFPEVRTERLLLVLVGEADAVAVSNNVWVRLPDAPTDLELRLDGAVVWSAPGP
                     AEPN"
BASE COUNT         1542 a         3142 c         3088 g         1543 t
ORIGIN      
        1 cgtcgcccca ggctccgaac gtgcagtgac gagcgctgcg cgcctcgagt ccgcgctcgt
       61 tcggctcgcg gctgcccgtc gtgagcgaac cgcgagcacg aaagctacgc ccacggcggc
      121 tcgaggcctc ccgctcgggt ccgagggatc gagtggcggc gtcgcgccca gcctcggcgg
      181 cgggctacag gccctcgcgg cgcgcttcgg cacacccgaa tctgacggtg cgcggcccat
      241 gcccacgagc gaatcgtcac gacaggaccc gtcggcgcgg cgtgcggcga ccggcttcga
      301 ggacggtgcg gaagcgttgc ccccgttacc gcggccactt ctcgaacgac tctcgcgcaa
      361 cgagctcacc cgacgcttgg acgaaatctt gcggcatgag gccgaacgct cgggcgtcga
      421 cttcaatcgg aggatgccgt gacgctgtcg cggccccgtg cgcgcgtgac cctcgacggt
      481 caagcgttga gctctgccga ggcggcgctc gcgcgcgtgg aggtatcgct ttggcttggc
      541 gctcgtcacg accacgcgta cgttgcgctg tggcccgact cacgcttcgc agacgtcgcg
      601 ccgggcgcca cgttgagcat cgcgctcggc gaagatggtg cggaggaagg ggtgttctct
      661 ggcacggcga cgcgagtgac cgccggcgcc gatggcatcg agatcgacgg actctcgctg
      721 agcgcgaagc tctccaaatt ccgtcgttcg cagacttacg tcgagcagac ggtggcggac
      781 atcgtgcggg acctggcggg cagcgtgagc gtcgacagtg ttgccggcga tctcaagctc
      841 gcatcgtaca gcgtcgatca gcgtcgttcg gcgtgggatc acatcgtcga actcgcgacg
      901 ttgacggggg ccgacgtcgc ctccacggca tccggggcat tgcgcttcgt tcgaccgcgg
      961 accggcacgc ccgacgtgac gctgcgctac ggcgctgacg tactcgcctg ggagatcggc
     1021 cccgtcgtcg aacccggcgt gccgaaggtc gcagcgcacg gcgcaggcag cgaagccgga
     1081 tcggagcaat ggcactgggt gctcgcgtcg ccgcctgccc aaggcaatgg cgatggccca
     1141 gtcgacgtag tccctgcgtt cacgacgcgc gacgcagccg agcaactcgc gcgcgctcgc
     1201 gccgaagccg gaaagcgcgc cgccgtctcg gcgcgcctga ccacgctcgg tcgccacgat
     1261 ctccgccccg ggagcctggt tcgagtttcc gacctgccga cgggagacgc tggcgtcttg
     1321 cgggtactcg gcgtggagca ctgcctcgac tcgcgcaccg gcttcgtaac caacgtgacg
     1381 gcggagggag caggatgagc gacatcatcg ggacactgcg cgcgatcatt cgcgacgagc
     1441 tagcgcgtca gcgccacgcg gagctcggcg tcgtcacgcg ggtccatgcg aaagatggag
     1501 acgacagcaa gaacaaccac caggtggatc tcaagctgcg cggcagcgac gtcgagctcc
     1561 gccgcgtgcc cgtcgcggtg ggccgccttg gcgtgtccgc tctgccgaac gaaggtgatc
     1621 tgatgatcgt cgtgttcgtc ggcggcgatc tgaacggcgc ggtcgcggtc ggctgcgtct
     1681 atgatcaaga gcgccatccc ccggtcgccg cgctgcacga gctcgtctac cagccgcccg
     1741 atccagaagc gtcgggggtg aagcggctgc atctcgagct ccaaaatggc agcaccgtga
     1801 cgctcgacga cgaatcgctt caggtctcgc tcggcgatac gtcgctcgtc atcaacaagg
     1861 acggagactt gagcatcgaa gcggcgggcg atctcaagtt caaggccggc ggcaaagtaa
     1921 ccatcgaggc gagtggagac gtcaccatta ccgcgcaggg gaatctgaac gcgaaaggga
     1981 cctcggcgtc tctggaaggc cagagcgaag caaaggtcaa aggcgccacc ctcaccatcg
     2041 ccggcaacac ccaattcagt ccttcgtaag gagcaacatg ccaggtccac ccgtttcagt
     2101 tggttgttcg gtgatgttgt cgcccggcgc ctccggtccc cccgacacgg gcgtggtgac
     2161 gaccattctc cagaccaacg tcgtcgtcgg tggcttgccg ctcgcgacca cggggagcct
     2221 gtgtcagatg gtgaactccg tgtcgggggc tccgtacgtc ttgccgatcg gtcaaggcgg
     2281 ctcgtcgggc gtcggcatct cgaaccaggc gctcgtccgc gtcggtgatc ggatcccttc
     2341 ggggcccggc atcctgacga ttctgggccc gccggcggcg ccattcatca acgacaccaa
     2401 ctcaccctga tcggcacggc gtcgatcacg tccccaggac tccgatgaga ccatgaataa
     2461 cccccgctca ctcgacaaga tcgcccccct gttcgggaaa gatctcgcgc tagactacgc
     2521 gcagtccggc ggtttcttcg aagacgccga tctgcggagt ggccgccgtc ccaacgcacc
     2581 tggaatccgg cgtgaccttt cggtggccag cggcatcgaa aacatcactc aagcgatcgc
     2641 caaccgactg aagacgaggc gcggtgagct cgccgcgctc ggtcacccgg aatacggttc
     2701 gcggcatcac gagctcgtcg gggagcccaa cgtcgagcgg acgcgcaacc tcatcaagtt
     2761 gtacgtactc caggcgctgc gccaggagcc acgcatcgag aaggtattga aggcgacggt
     2821 tcgtgccgaa cacgagcctc cccgcgaggt cgtgcgcgtc gagctcgagg tacgcgtcat
     2881 cggtgagcca gtgccgttga atttggtcgt gccgttttcg ttggaggtga cgctgtgagc
     2941 tacgcagccg aaccgtactc gcagttcgtc gacgatctgc tcacatcgct caccggcggc
     3001 gtagctaggg agcaattcgt ctttcgagca gaagaaggct cgtttcggct ctctccgccg
     3061 gggccaatcg tgccgagctc gctgagcgtc ttcggccagt ctgcaggcgc ttttcagcgt
     3121 tttcagcccg accgcgattt cgtcctcgca cccgacagct cgctctcgtg gcagacggcg
     3181 gcggacggag ggcccgcggc ggacgcgatc tggcccgacg aggggacacc gttcttcgcc
     3241 aactacgatc acaccggtcc gctcggctct gcaccactgc tcagtgaccg gaaccccggt
     3301 agcgtggtgc gccttttggc cgagagcttc ggtcgcgaat tcgccgtcgt gtcgaagcag
     3361 ctcgaagcca tccaccgcgc cggcttcttg agcaccgcca gcgggcgaga cctcgaccag
     3421 ctcgtggcgc tcgtggggct cacccgccgc acgcgcgcgt tcgcaacggg ctcggtgatt
     3481 ttctcgagtg ccgcgcctgc tcgcgccgac atcaccatcc aagctggaac gagactgagc
     3541 acggccgaac cgcctgccat cacgttcgag acgaccgaag accgcacgct gcatcgaggt
     3601 gagctcacgg tggaagcgcc gatccaggcg ctcgtcagcg gcggcgcggg agtcgtgccg
     3661 gcgcgtgcgg tcagcgtgat ccaccggccg atttttggca tagagtcggc gtcgaaccca
     3721 caagccaccg agcagagcgg cgacgacgag tcggacgcgg ccctgcgcgc tcgcgcacgc
     3781 cgcgcgctcg agacggcggg tggcgccacg accggggctt tgcttggtgc ccttgcgtca
     3841 ctccccggcg tgcgcgacaa cgacatccgg gtggcggaag atcatctcgc gcgcccgggg
     3901 gtggtgacgg tcaacgtggc ggctccgctc gacggtgcgg ctgcgttgcg ggccatcctc
     3961 ctcatcgatg aaaagcgtcc agcgggcgtg cgcgtgctca cgaacctcga cgctccggcc
     4021 ccgcctggcg ggccgtctgc gccgttgaac gtggtcgacg atgcggacgc ggaagatacg
     4081 ggcgagctgt tcgtcgacgg cttgtatctg ccggtgcagg tccgcatcgt gttgctcgcc
     4141 gcggccgcga cgctctccgc caacgagcgc agcgcgctca ccacggctgc ggcgggggtg
     4201 gcccggcgct tcgtggccga agccggcatc ggtgaagtcc tcgtctacaa ccgcctcgtc
     4261 gccggcatca tggcgctcga aggggtgctc gacgtagcgc tcgagctttc gcccaaggtg
     4321 ctcccgggcg aacccgtggg tccgcgccac caaaacctga tcccaccgga gacattgcgc
     4381 ccgcgcctat cggacacgga cctcagcgtc gagatcgcgg gcgagctcgt ctcgttcgat
     4441 gtcacggtca cggtcgcgct caccccgctc ggacaatcag tcgatccgag cgtcagcctc
     4501 gaggtcgcgc ggctccaaat cgccgctcag ctgcaggaca agattaccgc catcgtcggc
     4561 ccaatcacgg ccacgggctt gcgggacaag gtagtcccaa ccgagtactt ctcgttgtcc
     4621 gcgctccact atcgagtcca gtacttggag gccggcctca gtatcaacgt cgatgacccg
     4681 cagatccagc tggccgagct cgaacgagcc tggatccgca cggtccggct gtcagccgag
     4741 agcggttgaa catgacggga cgcgcatttc aagtcttatc gcgcttgccc gcgcacctcg
     4801 agcccacgcg cccgggcaag cagctcagcg ccgtcgtcga agcgctcgtc gtggatctgg
     4861 agcgcctctc cgccgacctc gcgggcgttc gccgttcgca tcggctcgcg cacgcggaca
     4921 cgctcaggga cgttctgctg ctcggcggta tgcacggcct ggctcgaccc gacgtcgagc
     4981 tcgtgatcgc gcgaatcgaa gcgactcgca gcatcgcccg agcgctcgaa gcagcggtcg
     5041 tagcgaaggc aactgccgag cgcgacgagc tggccgagag attgttcact ctttggggga
     5101 tccaaggcgg ctcgccgcgg ctcgccgatt tggcgccacc gttcgcgccg ggcgaaacgc
     5161 cgaacctcga cgcggccgcg cgcgccttga tcgaatcggt gcgcatgtcc tttgggttct
     5221 ccgcgttgct cgacggggtg cgtcgccgcg tcgagcgggt ggcgcgcctt cacgccgagg
     5281 gcaacggcac cgtctacgcc ttgatcgaag ccgcagcctc ggtgctcgat ctggagctcg
     5341 acgtcgaggc caaccaacgc gtcaaagccg cttctcggcc ggtagtcaga gccagcaccg
     5401 gcgggacccc aggcacggcg acgtggtcct acgttgtcgt tgcgcgcccg ctcgtccgca
     5461 acgtggcgcg gaacagccgc ctcgcggtcg tcgtgcgcgg ccccgcggcg ttgtccgaaa
     5521 ctgacttcgt aaagctagag tggtcgagcg tgcccgacgc gcgtgactat ctcgtttacc
     5581 gcgtgcggag cggtggcgaa ccgagcacgg ttggtctttt gacgccgacg ccgctcgagt
     5641 cgacgaccac gacctttcgc gataccggcg ttcccgccgt cgcaccggcc accccgttgc
     5701 ccgaaacgga cgatggctta tttcacagcg cagatcggtt ctggcactcg agtttcgtgc
     5761 gcgaccgcat ccggccgatg cgcagcgtcg agcccgcgtt gcccccgcag ttcgtggcgc
     5821 tcgattcgac gatcgtactc gatgatttcg caaccgcgct cgggcaagca ccggcggcgg
     5881 tactcgaacg cgccggcgct ctagcggccg cgggggcgtt gctcgacggc gaggttcacc
     5941 ccgatctggc actacgcgtc gccaatcgct tgggctcggc actggtcttt cggcggccga
     6001 tcagcgtgcg cgcccttgct tccgcgctcg cggctcctcg cgccgacgtg ttccgcgctc
     6061 tcgcggcgct attgagtgaa ggcgccaatg cacgaatttc gatcgagctc agcaccgccg
     6121 atccgctcgc cgcctccttc ggtttcttgg tcgtgcagtc gcggcggctc ttcgtggacg
     6181 acggcgtgcg gctcggcgag ctcgagcgga ggactggagt cgagctggac cagctcgtct
     6241 ctgaattggt gaagctcgac gtcccgggcg cgggggcgga gacacgactc acgttcgccg
     6301 aggcggcgcg actcttaccg agggtcggat tcagagcggt gcgtccgcgg atattcctgc
     6361 tcgacgcacg cgctccaatc agcgtcgccg agctttcggt gcgggccgcg ttgccggcga
     6421 gccggctctt gcgcgacctc gcgctcctcg gcgtcgccgc gccgacgccc gggacgctcc
     6481 tcgacgccga tcgcgttgcg cagctactgc gcccgcaccg cttcgcggtg cgagagatcc
     6541 tcgccgcgag tgacgacctc gtcggcatcg aagagaaccc gatgcgggac gaagaaaccg
     6601 cctcgacgcc gcgcgtgcac accgagctgt tccccgtatt tcgtcgcggc tttgggcggg
     6661 tgaggctcga ggcgcgcatc gtcgggatcg aaacgcgtac ggtgggcccc atgctcgtca
     6721 atcgcgacga agggcacggg atcggcctcg cgcgggcggt gccacaagga aagatgctcg
     6781 tgttcagcgc ggatgggcgg gcgcggctca ccagcgtcga accggcagag ggcgagagcg
     6841 acgaggacgt caccggcttc gcctattcgt ggcagggcgc ctgctttgcg ggcacggacg
     6901 acgaccctgc gaacccgcgc gacttcttgt tcgatggacc agacgttccc gagaaccgtc
     6961 gcgcgacgtt cgccgtcgca gccccagcgg gcgcgctcga tcccgagttc ccgttccctc
     7021 acgcgggcgc accaatcgcc atgcccggcg tgaacgtcgg ccaaacgcgc ttcgcgttct
     7081 tcgtgcaaga agcacatttc gccggcctcg acgagtcggt cgcgccgccg gcggcgatcc
     7141 ccccggttcc acgtcccaaa gttggattcg cggacggggc cgtgttcggc gtcggttccg
     7201 gcgaagagcg cgcgccagcc gcggaagtat cgctcgcttg gcgagagcac gaagcttatg
     7261 cggtgcgagt gctgatcccg aagcgcttct cggcgctcga caccgaaggc gcgccaaccg
     7321 tcgccgaacg ggtccgcgta gcactcgaac gcgtgcgccc cgtcggcgtc gaagtgcgcg
     7381 tcgagtatca agacgatcgc tggattctcg gcgaggcggc ggtgtccgcg gatcgcacgg
     7441 atccgatcct ggcgctgatg ggcggaacgg cgctctggcc gccaccaacg ggctgaacac
     7501 gaaagtgaac aaaggaaatt gcgaatgttg aacttcagca agacccacgt gaaccccggc
     7561 gagccagtga cggcacgcgc ctggaacgac atcgtggacg gcctgttcga ggtccaggcg
     7621 ctgctcaagg cggcgggcgg gacggcgacg gttcaggtca ccggcgctgg cgtcgattca
     7681 cgccgcgtgc gcgtcacggc cacgagcgct gacggcaccc ccgccgaagc attgcgcccc
     7741 atcccgccga gcaccgactt catttttcct cgactggccg ccggcgcgta ccagatccgc
     7801 atcgaggcgc ccggtttcga tgtcgcgacg ggagcggtga cggtgggcga agacggctcg
     7861 gtttctccca agccactcgc gatcgctctc acggcgaacc gcgtcgtgat gcccagcgtg
     7921 tttggactca aactgccgga agcggtcgcg ctgctcggct cgatgcagct ccggatcctg
     7981 gatgtgtcgg ggaagagcct cccgaagacc ggcttcgaat ccacgtacaa caacgccccc
     8041 gtgctcgtgc aatcaccgtc cccgggagag ctcgtgcaag ccgaaggagc gttcttggta
     8101 gtggccgctg gtctggtggt cgagcccctc gtcgtggttc cgaacctgca aggtaagacc
     8161 tacgcgcaag cgagggctgc gctcgaaact ctcgggctga agatcaacgt catcaagtga
     8221 ggagggacaa gtggcaacca agatcaaggc cagttactcg acagaggtcc aagacaagct
     8281 ctctcgctac gacacggtca aagccgaagc ggaagtggtg ttccagagcg tggcgccagg
     8341 caccacgatc cggcggggcg aaaccgtcaa cgtgcggctc atctcgtcat cggacgtacc
     8401 gctgcatgat ctcgtcaagg agggcctccc cgagcgattc accaagttga ctctggctga
     8461 cctggacgac gtaggcggag accccgagat catcggcatc atcaaggatc ccgccaagct
     8521 caaggaccca accgagcgca tcaaagccaa aaatagcctg gaaaagagct tgaataccaa
     8581 cctctccgag caagaagccg acgccatgtt cgggatcgtc ggagccgtgt tctagcgggt
     8641 cgcgctacca tggcccaagc atcttcagcc acgttggtcg gaaatttgga cgccatccag
     8701 gaggcgtccg agttcgcgct gttcgaacag aatctcgtct acgagacgct ccacgccccg
     8761 ctcgttcgca ctgacggcat cagctcgcgc accgtggtga agcgcttgca ccgcaacgcg
     8821 ctcgtgcggc gactgaacta cgtcgtgtcc gctgggcggg cagcggcggc gctcctcgcc
     8881 aacgccggac aggtgcggct gcaagagagc acgccggggc tcacgcaggt ggtcgtcgat
     8941 ttcggcgttc cgcgcacggt cgcgagcgtc ggcgcactcg acgcgacggg cggccctgaa
     9001 ctcaagatct tgagcgtttc cgtttgggac ggggcgaagt tcttgaagac cgcgcactac
     9061 tcagcttcgt gggcacaagc ctatcaaaac ggcacggtcg tggacatcgt tacggcgtcg
     9121 gatccatcca ctgcgaagat ggcaatcttc ccagaagtca ggaccgagcg gctgctgctc
     9181 gttctcgtcg gtgaggcgga cgcggtcgcg gtgtcgaaca acgtttgggt gcgattgccc
     9241 gacgcgccga cggacctcga gttgcggctc gatggcgccg tcgtctggtc cgcgccgggt
     9301 cctgccgagc cgaac
//
LOCUS       DASTKS010001164         5726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_853796, whole genome shotgun sequence.
ACCESSION   DASTKS010001164 DASTKS010000000
VERSION     DASTKS010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853796"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFQ35_24785"
     CDS             complement(<1..958)
                     /locus_tag="VFQ35_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933953.1"
                     /translation="MATEEPPKPETANAAADEAEALGAGEELARSGESVTAGKRLVPP
                     PKPRFPDAEIGTGPAPRAPMIVIGGTPSPPQARGEAQAWTRPSEPPPAVRSSPPATAN
                     KRPSQPAVDLRSKLGIQRAPDARPSPSRPVQEGPPPVAPKEVRSPAPPPRSEPRAALP
                     PVAPVGNASPPLPPTASSVPAAAPPSPSSPQHAVPLPAFALPEDALREPPLPSFALPV
                     EAPAKAPPPASERPQEKPLPSFALAEEAAVAPAQAQEDEPTPKPVAEKAEAPLPSFAL
                     EASLAEPTKERPGALEAALKEAFPVGHAEIPPPPKIEAPAAEP"
     gene            complement(984..1805)
                     /gene="asd"
                     /locus_tag="VFQ35_24790"
     CDS             complement(984..1805)
                     /gene="asd"
                     /locus_tag="VFQ35_24790"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241644.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HET9933954.1"
                     /translation="MSLSTFAAAQLLRALPRVRLSRAVGRLCDRELPSPVAEQLTRVY
                     CRAYGVNLDEAEPCGGYPSFDAFFTRGLRPGARTVSPDALVSPADGRLSAAGRIDAGA
                     RLFVKGQAYEVGELTGDPSDAARYTGGEFAVVYLAPGDYHRVHSPAEGEVTLVRGIPG
                     DLYPVNRIGELHVPRLFVRNSRVQIVLETRGLGRVSVVMVGAVIVGRITVSGVEGNDV
                     PPGEHRFDPPRSVARGDELGMFHLGSTVVLLLEPGVSVTRQLGKIRYGDSLLKAE"
     gene            complement(2002..3552)
                     /locus_tag="VFQ35_24795"
     CDS             complement(2002..3552)
                     /locus_tag="VFQ35_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933955.1"
                     /translation="MRRSLSIAAVAGALFANASSAAPDTTPLGELRRIERVANAVTEL
                     DVARAEKLLAEAGAESAALSFERARLSVYRGDCAVAAVTLARPELLETKEGMGLKKLA
                     ESCARATAAGVVIEDKQRGLWLRLQDDADRALAPFLFDVAVKARDAITRAVGVDLPRP
                     LRIDLVRDLFSLSAVSGLPLEAAETTGTLAVARWGRVILLSPRATPLGFPWEDTLTHE
                     ITHLVVTRATRDHAPLWLQEGMAKRLETSWRAPRPFDEPGWADALAYRAMLEGRSVGI
                     DQLGPSIAMLPTPEAASIAFAEVTSFVSYFARTAGEPALHLLFADLRGTGEHGADVAL
                     RSVTGQTLSEWNRRWQSALLAGPPPPSQPRFEHGIGGGRELARRVRLSDLLANQGKSR
                     SAADEILPAITAEQREPAVRWRGARALVESGEGTLARPTLGTLAEISSVHGPWFGLHG
                     RLLRESGQPSEAEEAFRIGIAVDPLSEETACEGARAPRGEKSAQGTLPDDPARRALCE
                     AARARPRD"
     gene            complement(3549..>5726)
                     /locus_tag="VFQ35_24800"
     CDS             complement(3549..>5726)
                     /locus_tag="VFQ35_24800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HET9933956.1"
                     /translation="HGIPLRAGRRLVLTDGEHETEFVDDGNGGVVARFKLDADRTMYV
                     AARFGGVVIREPDRLEIKALRDEAPKIELEGAPRTLSLEEVEGVELRYAVQDDHGLRQ
                     IDLVLRSGSHEDRRSLVKLDGQSREEQGAHALSPRDPFLRRAFLPIIATIQARDNDPT
                     HAARWGESKAITILPPDVGAPQAQRYRALKNGENQLIALLDLALGDERERRAHPGAPV
                     PKERRDELLKQKVEVVNTLRQAVTASYGSLKVSSGLAAFVLGQARLLDSLRDEPRRRI
                     EDALLALDAGLMALGQRDATAVAKRLGDLAEEVADAARATFASEQRELGKKRFDTALA
                     ALDRGVANLLVLGALGADVGSVAQGEIRRIRRAAEANSFSDAELAARHLAARLRRPSP
                     SFSAGGGGGGGVESGRGSQGGQGSASDADQQFDQLMHELERLAEDHAEQIRQVEGTLS
                     EADQSAGSEDLKREAAERAARLRSKLADLPEPGAPQGSGRAAAAIAREHLNAMAQNLE
                     RLSLKDALENGKSARGELTHAKRRSQAPESASDWLDDNAVSEANRELADDLGWTEQAL
                     EKMKRERARALEKDLNSAADREDSLARRASNLSGRGEHSEAQLPEDVQESLERAESTM
                     RQAARALRTGETDEAQNLQAEAQRLLEHASPGRTAEEQEQGDSRGGSGDDKGEPATQG
                     NVPGVNKRRRSEDFRKRVLEGLARERRGALAPAVERYAEGLLE"
BASE COUNT          815 a         1964 c         2004 g          943 t
ORIGIN      
        1 ccggctcggc tgcgggtgcc tcgatcttcg gcggtggtgg gatttcggcg tgaccgacgg
       61 ggaacgcctc tttcagtgcg gcctcgagtg cgccgggtcg ttccttggtg ggttcggcga
      121 ggctcgcttc gagcgcgaac gaaggcagcg gagcctcggc cttttcggcg acgggcttcg
      181 gcgtcggctc gtcctcttgc gcttgagcgg gcgcgacggc ggcctcctct gccaacgcaa
      241 acgacggcaa gggcttttct tgcgggcgct cggacgcggg aggcggagcc tttgccggcg
      301 cttcgacggg cagcgcaaaa gacggaagcg ggggttcgcg cagcgcgtct tcgggcagtg
      361 cgaaggccgg aagaggaacc gcatgctgcg ggctcgaagg cgaaggaggg gccgcggcgg
      421 gaacgctcga agcggtcgga ggcaacgggg gggaggcgtt accgacgggg gccacgggtg
      481 ggagggctgc tctgggttcg gagcgcggag gcggcgccgg cgagcgcacc tctttcggcg
      541 cgaccggggg cggcccttct tgcacgggtc gcgagggtga gggtcgcgca tctggagcgc
      601 gctggattcc aagcttcgag cgcagatcga ccgccggttg gctcgggcgc ttgttggcag
      661 tcgcgggcgg tgatgaccgg acggcgggcg gcggctcgga tggccgagtc cacgcttgag
      721 cctcgccccg ggcttgcggc ggactcggag tgcccccgat gacgatcatc ggtgcgcgag
      781 gagcagggcc ggttccgatc tccgcatcgg gaaagcgcgg cttcggcgga ggcacgaggc
      841 gctttcccgc cgttacggac tcgcccgagc gggcgagctc ctcccctgcg ccgagcgctt
      901 ctgcctcgtc ggctgccgcg ttggcagttt cgggcttggg aggttcttcg gtcgccatcg
      961 ctggtgtgct gctcagcgcg aggtcactcc gccttgagca gcgaatcgcc gtaacggatc
     1021 ttgccgagct gccgcgtcac cgatactccc ggttcgagca gcaacacgac agtggacccc
     1081 aaatggaaca tgccgagctc gtcgccccgc gcgacgctgc gcggcgggtc gaaacgatgc
     1141 tctccaggtg gcacgtcgtt gccttcgacg ccgctcaccg tgatccgtcc gacgatgacc
     1201 gcgccgacca tgacgacgct gactcgcccg agcccgcgcg tctcgagcac gatttgcacg
     1261 cggctgttgc gcacgaacag gcgtggcacg tggagctcgc cgatgcggtt caccggatac
     1321 agatcgccgg ggatgccgcg cacgagcgtg acttccccct ctgcgggcga gtggacacgg
     1381 tgatagtcgc ccggcgcgag gtaaacgacc gcgaactcgc cgccggtata gcgagccgca
     1441 tcgctcggat cgcccgtgag ctcaccgacc tcgtacgcct gccccttgac gaacaagcgc
     1501 gcacccgcgt cgatacgccc ggccgccgag agccggccgt ctgcgggact gaccagcgcg
     1561 tctggactca cggtgcgggc gcccggccgt agcccacgcg tgaagaaggc gtcgaagctc
     1621 gggtagccgc cgcatggctc ggcttcatcg aggttcacgc cgtacgcgcg acagtagacg
     1681 cgcgtcagct gctccgcgac cggactcgga agttcacggt cacacaaccg acccacggcg
     1741 cggctcaaac gcacgcgcgg caatgcgcgc agcagctgcg ctgctgcgaa cgtcgacaag
     1801 ctcactcgat cacctccgcg cgcactcgcc gcccgcaaca tgccccgccc gacgcgccgt
     1861 gaaagcggcc gtcatcggcg cccaagcggc actcaaacac caacgtgaag tacgcgattc
     1921 gttctgcgag cacgagcagc gagccggggc ggccaaaaaa cagtgcgagc tccccatctt
     1981 aggcggtccc gggagggccg gtcaatctcg ggggcgagct cgtgcggctt cgcacaaggc
     2041 gcggcgcgcc gggtcatcgg gcagggtgcc ctgcgccgac ttctcaccac gaggtgcgcg
     2101 agcgccttca cacgccgtct cctcagacag ggggtcgacg gcgatgccga ttcgaaatgc
     2161 ttcttctgcc tcacttggct gcccgctctc gcggagcaac cgaccgtgca gtccgaacca
     2221 tggtccgtgt acgctgctaa tttccgccag agtgccaagg gtcgggcggg ctagcgtgcc
     2281 ttcgccgctc tccacgaggg cccgcgcgcc gcgccagcgg acggcgggct cgcgttgctc
     2341 ggcggtgatg gctggcaaga tttcgtcagc ggccgacctc gacttgccct ggttcgccaa
     2401 caagtccgaa agccgtacac gccgggcgag ctcgcggccg ccgccgatcc cgtgctcgaa
     2461 gcggggctga gatgggggag ggggccccgc gagcagcgcg ctctgccagc ggcgattcca
     2521 ctcgctcagc gtctgtccgg tcacgctacg cagcgccacg tccgcgccat gctcgccggt
     2581 gccgcgcagg tcggcgaaga gcaggtgcaa cgcgggctcg cccgcggtcc gcgcgaagta
     2641 gctcacgaag ctcgtcacct ccgcaaaggc aatcgaggcg gcttccggtg tcggcagcat
     2701 cgcgatcgag gggccgagct gatcgatacc gaccgagcgc ccctccaaca tcgcgcggta
     2761 agcgagcgca tcggcccagc cgggttcgtc gaacggtcgc ggtgctcgcc agctcgtttc
     2821 gaggcgcttc gccatgccct cttgcagcca gagcggcgcg tggtcgcgtg tcgcgcgcgt
     2881 cacgaccaag tgagtgatct cgtgagtcag cgtgtcttcc cacgggaagc cgagcggagt
     2941 cgcgcgcggc gagagcaaga tgacgcgccc ccagcgcgcg accgcgagcg taccggtggt
     3001 ctcggcggct tcgagaggca accccgagac cgccgacagc gagaacaaat cacggaccaa
     3061 atcgatgcgt agaggtcgcg gcaagtcgac gccgaccgct ctcgtgatgg cgtcgcgcgc
     3121 tttgacggcg acgtcgaaga gaaacggcgc gagggctcga tcggcgtcgt cttgcagacg
     3181 cagccacaag ccgcgctgct tgtcctcgat cacgacgcca gcggcggtcg cgcgagcgca
     3241 gctctccgcg agcttcttca gccccattcc ctctttggtc tcgaggagct cggggcgagc
     3301 cagcgtgacg gcagcgaccg cgcaatcacc gcgataaacc gagagtcggg cgcgctcgaa
     3361 gctcagcgct gccgactccg ctcccgcttc ggccaacagc ttttcggcgc gcgcgacgtc
     3421 gagctcggtc accgcgtttg caacgcgctc gatgcgacgg agctctccga gcggagtggt
     3481 gtccggcgcg gcgctcgaag cgttcgcgaa gagcgctccg gcgacggccg cgatcgagag
     3541 cgagcgtctc attcgagcaa tccctccgcg tagcgttcga cggcgggagc gagggcgccg
     3601 cggcgctctc tcgcgaggcc ttcgagcacg cgctttcgga aatcttcgga gcgacgtcgc
     3661 ttgttcacgc ccgggacgtt gccctgcgtc gccggttccc ctttgtcgtc gccgctgccg
     3721 ccccggctgt caccttgttc ttgctcttcg gcggtgcgac cggggcttgc gtgttcgagc
     3781 aagcgctgcg cttcggcttg gaggttttgg gcctcgtccg tctctccggt tcgcagcgcg
     3841 cgcgcggcct gccgcatcgt gctctccgcg cgctcgagcg actcctgcac gtcttcgggg
     3901 agttgggcct cactgtgctc gccgcgtccc gagaggttcg aggcacgccg tgcgagcgaa
     3961 tcctcgcggt cggcggccga gttcaggtcc ttttcgaggg cgcgcgcgcg ttcgcgcttc
     4021 atcttctcga gcgcttgctc cgtccagccg aggtcgtcgg cgagctcgcg gttggcctcc
     4081 gaaacggcgt tgtcgtcgag ccagtcggac gcgctctccg gtgcctgcga gcgccgcttg
     4141 gcgtgagtga gctcgccgcg cgcgctcttg ccattctcga gcgcatcctt cagcgagagc
     4201 cgctcgaggt tttgcgccat cgcgttcagg tgttcgcgag cgatcgcggc cgcggcgcgc
     4261 cccgagcctt gcggcgcgcc gggttcgggc agatctgcga gcttggaacg gagccgcgcg
     4321 gcgcgctcgg cggcttcgcg cttgaggtcc tcggagcccg cgctctggtc cgcttcggag
     4381 agagtccctt ccacttggcg gatctgctcc gcgtggtcct ccgccaagcg ctcgagctcg
     4441 tgcatcagtt gatcgaactg ttggtcggcg tccgaggcgc tgccctgtcc gccctgggag
     4501 ccgcgacccg attccactcc tcctcctccg cccccaccgg cgctgaacga aggcgaaggg
     4561 cgacgcaagc gcgccgcgag gtgccgcgcc gcgagctcgg cgtccgaaaa gctgttcgct
     4621 tcggccgcgc ggcgaatgcg ccgaatctca ccctgcgcga cgctaccgac gtcggcaccg
     4681 agcgctccga gcacgagcag gttagcgacg cctcgatcca gcgcggcgag cgcggtgtcg
     4741 aagcgctttt tgccgagctc gcgctgttcg ctcgcgaacg tcgcgcgcgc ggcgtcagcc
     4801 acttcttcgg ccaaatcgcc gaggcgcttg gcaacggcgg tcgcgtcgcg ttgtccgagc
     4861 gccatcagtc cggcgtcgag cgcgagcaaa gcgtcttcga tgcgccgccg cggctcgtcg
     4921 cgcaacgagt cgagcaggcg agcttgcccg agcacgaaag cggcgagccc gctcgagacc
     4981 ttcaagctcc cgtaactcgc cgtgactgcc tggcgcaagg tgttcaccac ctcgaccttc
     5041 tgcttcagca gctcgtcgcg gcgttccttc ggaacgggag cgcccggatg cgctcgtcgc
     5101 tctcgctcgt cgccgagggc gagatcgagc agtgcgatga gctggttctc gccattcttc
     5161 agcgctcggt agcgttgggc ctgcggcgca ccgacgtccg gcggcaagat cgtgatggcc
     5221 ttgctttcgc cccagcgcgc ggcgtgcgtc ggatcgttgt ctcgggcttg gatggtggcg
     5281 atgatgggga ggaacgctcg ccgcaggaaa gggtcgcgcg gcgagagcgc atgggccccc
     5341 tgctcttcgc gcgactggcc gtcgagcttc acgagcgaac gacgatcttc gtggctgccg
     5401 gagcgcagca ccagatcgat ctggcgcagg ccgtggtcgt cctgaacggc gtagcggagc
     5461 tcgacgcctt cgacctcttc gagcgacagc gttcgcggcg cgccttcgag ctcgatcttc
     5521 ggcgcctcgt cgcgcaatgc cttgatctcg aggcgatcgg gctcgcgtat cacgacaccg
     5581 ccgaaccgcg cggcgacgta catggtgcgg tcggcgtcga gcttgaagcg agcgaccacc
     5641 ccgccgtttc cgtcgtcgac gaactctgtt tcgtgctctc cgtcggtgag aaccagccgg
     5701 cgaccggcgc ggagtgggat tccatg
//
LOCUS       DASTKS010001165         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1302875, whole genome shotgun sequence.
ACCESSION   DASTKS010001165 DASTKS010000000
VERSION     DASTKS010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302875"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VFQ35_24805"
     CDS             <1..1595
                     /locus_tag="VFQ35_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET9933957.1"
                     /translation="REANVHRLAEVSTAIIDQCVAQGVPFAREYGGMLDNRSFGGTQV
                     QRTFYARGQTGQQLLLGAYSALERQVAAGRVKQYSRHELLDIVIADGRARGIIARNLV
                     TGEIERHGAHAVLLCTGGYGNVFFLSTNAMGSNVTAIWKAHKRGAWFGNPCYTQIHPT
                     CIPVHGEAQSKLTLMSESLRNDGRIWVPKQQGDKRRASDIPEAERDYYLERRYPAFGN
                     LVPRDVASRAAKERCDAGYGVGHSGMAVYLDFADAIKRMGEAQVSAKYSNLFEMYEKI
                     TGENPYKVPMRIYPAVHYTMGGLWVSYELQTTVRGLFALGEANFSDHGANRLGASALM
                     QGLADGYFVIPYTLGNYLADDIQSGKLDTNRREFAEAEAAVKDRIAQVMNIGGTIPVD
                     KFHRELGKVMWDKCGMGRNAQGLEQAVGEIRGIREDFWKRARVPGNANEFNVELEKAL
                     RIADFLELGELMCIDARHRNESCGGHFREEFQTEDGEALRDDPNYSYVAAWQYAGPGE
                     APVLHKEALNYQNIKVQARSYK"
     gene            1620..2378
                     /locus_tag="VFQ35_24810"
     CDS             1620..2378
                     /locus_tag="VFQ35_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET9933958.1"
                     /translation="MSNNLSLTLKIWRQKNAQDPGGFVTYSVSNVSSDSSFLEMLDVL
                     NLELVEKGEEPVAFDHDCREGICGACSMMINGRPHGPERGTTCQLHMRAFKSGDTIVI
                     EPWRAGAFPVIRDLVVDRSSFDRIIQAGGFVSVNTGAPQDANALPVPKPDADEAFDAA
                     ACIGCGACVAACKNASATLFVAAKVSHLAMLPQGKVERSTRVIKMVTQMDEEGFGACS
                     NTGACSAVCPKAIGQHHIARFNREYLRAQLLSGK"
     gene            2505..3275
                     /locus_tag="VFQ35_24815"
     CDS             2505..3275
                     /locus_tag="VFQ35_24815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9933959.1"
                     /translation="MNDAAAKPTNQWAPKLYEDRARFVREGGLPVLDLLAPRAGERIL
                     DLGCGPGKLTREIAVRGASVVGVDSSREMLNEARAAYPELQFALERAEALPYEDEFDA
                     IFSNATLHWVRRADEAARAMYRALKRGGRLAVEFGGKGNVATVRHAVSAALGEADWEP
                     WYFPSVGEYASVLERAGFEVRFAHCFARPSPMPDAPEQSGVATWLSLFANELLSAMKP
                     ADRATLFARVEGDTRSALYRDGVWYIDYVRLRVEAYKP"
BASE COUNT          646 a         1114 c         1128 g          597 t
ORIGIN      
        1 cccgcgaagc caacgtccac cgcctggccg aggtgagcac cgccatcatc gatcagtgcg
       61 tcgcgcaggg cgtaccgttc gcacgcgagt acggcggtat gctcgacaac cgttcgttcg
      121 gcggcaccca ggtgcaacgc accttctatg ctcgcgggca aacgggccaa cagctcttgc
      181 tgggagctta ctccgcgctc gagcgccaag tcgcagccgg tcgcgtcaag caatacagcc
      241 gccacgagct cctcgacatc gtcatcgccg acgggcgggc ccgcggcatc atcgctcgca
      301 acctcgtcac gggcgagatc gagcgccacg gcgcgcacgc ggtcttgctc tgcacgggcg
      361 gctacggaaa cgtgttcttc ctctccacga acgcgatggg ctcgaacgtc accgcgatct
      421 ggaaggcaca caagcgcggc gcttggttcg gaaacccctg ctatacgcag attcacccca
      481 cgtgcatccc ggtgcacggc gaggctcagt cgaagctcac cttgatgagc gagtcgttgc
      541 gtaacgacgg ccgcatttgg gtgccgaagc aacaaggcga caaacgtcgc gcgagcgaca
      601 tccccgaggc cgagcgcgac tactacctcg agcgtcgcta ccctgctttc ggtaacctcg
      661 tcccccgtga cgtcgcttcg cgggccgcca aagagcgctg cgacgccggc tacggcgtgg
      721 gccattcggg catggccgtg tacctcgact tcgccgatgc catcaagcgg atgggcgaag
      781 cgcaagtttc tgcgaagtac agcaacttgt tcgagatgta cgagaagatc accggtgaaa
      841 atccgtacaa ggtgccgatg cggatttacc cggctgtcca ctacacgatg ggcgggctgt
      901 gggtgagcta cgagctccag acgacggtgc gcggcttgtt cgcgctcggc gaagccaact
      961 tctcggatca cggcgcgaac cgcctcggcg cttcggcgct gatgcagggt ttggccgacg
     1021 gctatttcgt catcccctac accctcggca actatctcgc cgacgacatc cagagcggca
     1081 agctcgacac caaccgccgc gagttcgcgg aggcggaggc ggcggtcaag gatcgcatcg
     1141 ctcaggtgat gaacatcggc ggcaccatcc ccgtcgacaa gttccatcgc gagctcggca
     1201 aggtgatgtg ggacaagtgc gggatgggcc gcaatgccca ggggctcgag caagccgtcg
     1261 gagagatccg cggcatccgc gaggacttct ggaagcgagc gcgcgtgccc ggaaacgcga
     1321 acgagttcaa cgtcgagctc gagaaggcgc ttcgcatcgc cgatttcttg gagctcggcg
     1381 agctcatgtg catcgacgca cggcaccgca acgaatcgtg cggcgggcat ttccgcgaag
     1441 agttccagac cgaagacggc gaggcgctgc gcgacgatcc caattactcc tatgttgcgg
     1501 cctggcagta cgccggtccg ggagaggcgc cggtactgca caaagaggcg ctcaactacc
     1561 agaacatcaa ggttcaggcc cgaagctaca agtagtcgcc ggcgctcatc aggatttcca
     1621 tgtcgaacaa cctcagcctc actctgaaaa tctggcgtca aaagaacgcg caagatcctg
     1681 gcggcttcgt tacgtacagc gtgtcgaacg tctcgagcga cagttcgttc ttggagatgc
     1741 tcgacgtctt gaacctcgag ctcgtcgaaa agggcgaaga gcccgtcgct ttcgatcacg
     1801 actgccgtga gggaatctgc ggcgcgtgct cgatgatgat caacggcagg ccgcacggtc
     1861 ccgagcgtgg caccacgtgc cagctccaca tgcgcgcctt caagagcggc gacaccatcg
     1921 tcatcgagcc gtggcgcgcg ggcgcgttcc cggtaattcg tgacctcgtc gtcgatcgct
     1981 cctcgttcga tcgcatcatt caggccggcg gcttcgtctc ggtgaacacc ggcgcgccgc
     2041 aagacgcgaa cgcgctgccg gttcccaagc ccgacgccga cgaggctttc gacgcggccg
     2101 cttgcatcgg ttgcggcgcc tgcgtcgccg cctgcaagaa cgcttccgcg acgctgttcg
     2161 tcgcggcgaa ggtctcgcat ctcgcgatgc ttccgcaagg caaggtggag cggagcacgc
     2221 gcgtgatcaa gatggtgacg cagatggacg aggaaggctt tggagcatgc tccaacaccg
     2281 gcgcctgctc ggcggtttgc ccgaaggcca tcggtcagca ccacatcgcg cgcttcaatc
     2341 gcgagtacct tcgcgcccag ctcttgtctg gaaagtagcg gcggcgggcc ccggtggccc
     2401 cacggatggg tcgcgcgacg cggagcacga tggattagga tcgctaatct gtcgttaaga
     2461 acgtttcact ttacctaaat catcgcgcgg cccaagttct acgaatgaac gacgccgcag
     2521 cgaagccgac taaccagtgg gctccgaagc tctacgaaga ccgagcgcgt ttcgtgcgcg
     2581 agggcgggtt accggtgctg gacctcttgg caccacgcgc cggtgaacgg attttggatc
     2641 tgggttgtgg cccaggaaag ctcactcgag agatcgcagt ccgcggcgcg tcggtcgtcg
     2701 gggtcgattc ttcgcgcgag atgttgaacg aggcccgcgc cgcgtatccg gagctccagt
     2761 tcgcgctcga gcgtgccgaa gcgttgccct acgaggacga gttcgacgcg atcttttcga
     2821 acgccacgct gcattgggtg cgacgggcgg acgaggccgc gcgcgcgatg taccgcgccc
     2881 tcaagcgcgg aggccggctc gccgtcgagt tcggcgggaa aggcaacgtc gcgaccgtcc
     2941 gccacgcggt gagcgctgcg ctcggcgaag ccgattggga gccttggtat tttccgagcg
     3001 tcggcgagta cgcgagcgtg ctcgaacgcg ccggcttcga ggtgcgcttc gcgcattgtt
     3061 tcgcgcgacc gagccccatg cccgacgcgc ccgagcagag cggggtcgcc acgtggctct
     3121 ctctcttcgc gaacgagttg ctgagcgcga tgaagcccgc cgatcgtgcc acgttgttcg
     3181 cgcgcgtaga aggcgacacg cgctcggcgc tctaccgcga tggtgtctgg tacatcgact
     3241 acgtgaggct tcgagtcgag gcgtacaagc cctaacaggg tgttgaagaa gtaagtgttg
     3301 gaggggagat gacagcgtca aggtctgcat gaggtcccca cccccgcccg tcctcgccac
     3361 cggtggtggc tgcgggcggt cgacccctcc caaatcgccg gctacgcacg gctacgccgg
     3421 ctcagggagg ggtagcagct tggccattcg gaccaggttg taggcagctg cgaccatgaa
     3481 gccag
//
LOCUS       DASTKS010001166         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1748359, whole genome shotgun sequence.
ACCESSION   DASTKS010001166 DASTKS010000000
VERSION     DASTKS010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748359"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFQ35_24820"
     CDS             complement(<1..221)
                     /locus_tag="VFQ35_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933960.1"
                     /translation="MKWRAPSRSPPESSVRSHPIRRKALALNFDLAKRFWLPCALATL
                     LSATSLASGRALDDWLIALIVSGRAREVG"
     gene            321..1526
                     /locus_tag="VFQ35_24825"
     CDS             321..1526
                     /locus_tag="VFQ35_24825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933961.1"
                     /translation="MNRQLIRARLSRGSLSSLTLLCVACVAPQDVSDEHKPSVTPSLG
                     GTTSEFGGAPSASGGFFSADGGVGSPTSSGGALLFGTGGFTSNVAGGAPSASGGASGG
                     KFSSGSGGTFSSGGTRSSGGVNAAGGSLSPSGGAPASAGSTSTSGMGASPKLPEVSGT
                     CPNFVTGTATIGGLGGISLQVGAKKAGTGSLLFYWHGTGSSAGEVNTSLPAAVRQEIL
                     GQGGIIVSFQGTTGTGGDCSGTATFSQDDFKIADLIAACAVRDYGIDPRRIYTTGCSA
                     GGLQAGCMGALRSSYIAAVVPNSGGEILPLPIQDAAHTPSVMTMHGGSSDVVIVQFSQ
                     TSAAYDKQMKGAGSFVVNCDHGGGHCAASGALYTAGWEFMKAHPFDVNPEPYGAALPA
                     SFPSYCKVY"
     gene            complement(1674..>2230)
                     /locus_tag="VFQ35_24830"
     CDS             complement(1674..>2230)
                     /locus_tag="VFQ35_24830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933962.1"
                     /translation="VASATGPLAFTIDAEFAYLGFAGGVRRSNDRGLSFTPLANAPTH
                     VGCVTSRASELYVCGFANNEFAVWSSPNQGDNFRSYLRFSEVTDGVACPSTSTVSASC
                     SAAIADWQVEHPPESVNAAGGRGSTKFSAQDVAGNTSSSGCQLSRTSKSVPTTSIEWC
                     LVLAGVSLAARWTQKRSRDSKSHG"
BASE COUNT          384 a          713 c          751 g          382 t
ORIGIN      
        1 aacccgacct cccgcgcgcg gccggagacg atgagcgcga tcagccagtc gtcgagcgcg
       61 cggcctgacg cgagcgaggt tgcggagagc agcgtcgcga gagcgcacgg cagccagaat
      121 cgcttcgcga ggtcgaaatt cagcgcaaga gcctttctcc gaatcgggtg cgatcgaacg
      181 ctcgattcgg gtggcgaacg ggaaggagcg cgccacttca cgaggtgcgg gtcccaccta
      241 attgcgcgcg cattcgaaaa gacccacccc ggaatcggct ccgggaatac cggagtgggg
      301 gagccttcgc gacaatcgtc atgaaccgcc aactcattcg agcacgactc tctcgcggca
      361 gcttgagctc gctcacgctg ctctgcgtcg cctgcgtcgc accgcaagac gtgtccgacg
      421 agcacaagcc gtccgtgacg ccgagtctcg gcggtacgac gagcgagttc ggaggcgcgc
      481 cgagcgcgag cggtggcttc ttctccgcgg acggcggagt cggaagtccg acctcgagcg
      541 gtggggcgtt gctcttcggg acgggcggct tcacgtcgaa cgttgccggt ggcgcgccca
      601 gtgcctccgg cggtgcatcg ggtggcaagt tctcgagcgg gagcggcggc actttctcga
      661 gcggagggac gcgctcgagt ggtggcgtca acgcggccgg cggaagcctg agcccgagcg
      721 gtggagcgcc tgctagtgcc ggctcgacct cgacgagcgg tatgggcgcg tcgccgaagc
      781 ttccggaggt cagcggcacc tgtccgaact tcgtgaccgg caccgcgaca atcggcgggt
      841 tgggtggcat ctcgctccaa gtcggcgcca agaaggcggg caccggctca ctgctcttct
      901 attggcatgg cacgggctct tccgcgggcg aagtgaacac ctcgttaccc gccgccgttc
      961 gccaagagat tcttggacaa ggcggaatca tcgtgtcgtt ccagggaacg accggaaccg
     1021 gcggagattg ctccggtacc gcgacgttca gccaggacga cttcaagatc gccgacctca
     1081 tcgctgcgtg cgcggttcga gactacggta tcgatccgcg ccgcatctac acgaccggtt
     1141 gtagcgctgg tggcttgcaa gccggctgca tgggcgcact ccgttcgagc tacatcgcgg
     1201 ccgtggttcc aaactcgggc ggtgagattc tacccttgcc gatccaggat gcggctcaca
     1261 caccgtcggt gatgacgatg cacggcggat cctccgacgt ggtgatagtc cagttttctc
     1321 aaacgagcgc agcttacgac aagcagatga aaggtgcggg aagcttcgtc gtcaattgcg
     1381 accatggtgg cggccattgc gcagcctcgg gcgcgctcta caccgcaggc tgggagttca
     1441 tgaaggcaca cccgttcgac gtgaacccag agccctacgg cgcggcactc cccgcgagct
     1501 ttcctagcta ctgcaaggtc tactgagtcc gggagggcgc ccaggcggtc gcgcgcggcg
     1561 ctcgggcggc gctgcctcgt gcggcgctgc actgactttg ccgaagaagt gcggtgctgc
     1621 gtcgccgtcg acggccaggg aaccacctcc atcgcatttt cggcgtcgcg ggctcagccg
     1681 tgcgacttcg agtctctcga gcgcttctgg gtccaccgcg ctgcgagcga aacgccggcc
     1741 agtacaaggc accactcgat cgaagtcgtc gggacgctct tcgacgttcg gctgagctgg
     1801 caaccgctgc tcgaggtgtt tccggccacg tcttgcgcgg aaaacttggt ggagccgcgg
     1861 cctcctgcgg cgttcacgct ttcaggcggg tgctccactt gccagtcggc gatggcagcc
     1921 gagcacgagg cgctcaccgt gctcgtggaa gggcaggcga cgccgtcagt cacttccgaa
     1981 aagcgcaggt acgacctgaa attgtcgccc tgattcggcg aactccacac cgcgaattcg
     2041 ttgttggcga agccgcagac gtagagctcg ctggctcggc tcgttacaca ccccacgtgg
     2101 gttggcgcat tcgccagcgg agtgaagctg aggccgcggt cgttcgagcg gcgcactccc
     2161 cctgcaaagc cgaggtacgc gaactccgca tcaatggtaa atgcaagagg cccggtcgcg
     2221 ctcgcgactt
//
LOCUS       DASTKS010001167         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_407276, whole genome shotgun sequence.
ACCESSION   DASTKS010001167 DASTKS010000000
VERSION     DASTKS010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407276"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..138
                     /locus_tag="VFQ35_24835"
     tRNA            66..138
                     /locus_tag="VFQ35_24835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Lys,seq:ctt)
     gene            1073..1357
                     /locus_tag="VFQ35_24840"
     CDS             1073..1357
                     /locus_tag="VFQ35_24840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933963.1"
                     /translation="MLRQRLQVRLEHARRDLGQREVRELRVEEVLADDVPRSLSRGGT
                     VHVGLEPPAKELAERAATIFGRQRLEAEALTFGTKLELGGHALGFRLRAR"
     gene            complement(1443..1715)
                     /locus_tag="VFQ35_24845"
     CDS             complement(1443..1715)
                     /locus_tag="VFQ35_24845"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9933964.1"
                     /translation="MVHPERRVSQVAPGARPDELWTAADVAAYLKVSRSWVYHRAEAN
                     LLPYVRIGGLLRFEPEAVRQYARDGSSDMQRPRALPLRPRALRKGA"
     gene            complement(1702..1983)
                     /locus_tag="VFQ35_24850"
     CDS             complement(1702..1983)
                     /locus_tag="VFQ35_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933965.1"
                     /translation="MLVPYLRNLSGEARVVATGVHRKLNGKDAGKRSVFLEVELEDAK
                     EVEPEWIASMLEKFLKHVTEDAGAGGTFDIVKVEPAWESPDRAEKHGSP"
     gene            2344..2733
                     /locus_tag="VFQ35_24855"
     CDS             2344..2733
                     /locus_tag="VFQ35_24855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933966.1"
                     /translation="MEIQARSRVAREEWAKRVERWRESGLTSAEFAAELGINPRTLTY
                     WKWVLGKEALGEKREWPSRKRTVRAGRPPTRGAESRPALPELVEVHAAAGDARFELEL
                     GAGRRIRVPASFDATELRRLLDVLEAP"
     gene            2730..3077
                     /gene="tnpB"
                     /locus_tag="VFQ35_24860"
     CDS             2730..3077
                     /gene="tnpB"
                     /locus_tag="VFQ35_24860"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9933967.1"
                     /translation="MIPAAVRIFVSTEPIDMRQGFDRLAQAVRARLGEEPQSGSLFVF
                     ANRGATRLKVLWFDSNGYCLLYKRLHRAVFELPPSGGGAAVQVDARRLAELLRGAARE
                     RARKSQHRSAAAA"
     gene            3111..>3601
                     /locus_tag="VFQ35_24865"
     CDS             3111..>3601
                     /locus_tag="VFQ35_24865"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9933968.1"
                     /translation="MSEAALADDPEQLSAETALAELLARVATVEHERDEFKHERDEYK
                     KLYLFLREENERLKRGLIGQKAERLPRNDAQLSLAVLGLMLANGENPGAPPEDEPEQQ
                     IPAHTRKVPKRRTLPADWPRVTIELVPPEVEREGLDAFEIIGEERRGVLERRPASCVV
                     VEVV"
BASE COUNT          621 a         1154 c         1246 g          580 t
ORIGIN      
        1 cacacggagc cgtgagcgaa gcggttgaaa tgcccgcggg catgagctag aagccacgcc
       61 ctcccgggtc attagctcaa ttggtagagc agcggactct taatccgttg gcttcaggtt
      121 cgagtcctga atggcccacc ctcccaacct ccggaaactg aacgacgttc ggccgggggt
      181 cgtcgccatc actacccgct ctcgtcggat cgggtagcaa ccgggtaaca agagcgtcgg
      241 gaacgcctgc aaccgacggc gaatcgttgg acgccggcgc gagcttcagg cgatcgatct
      301 cgtcctgtag gtagctaggg acgaggtgcc cgtagacctc ggtcgtaatg cgcgggtccg
      361 agtgccggag gatgcgctgc acggcggccg ggttcgctcc cgccatcatg aggagcgacg
      421 ccgtcgtgtg gcgaaggtca tgaaaccgga tcggccggac gcgggccttg ggccagagct
      481 tgtgcccgtg ctcggggcaa cggcggagct ccccgtcctg cgcctcttcg gcgtggccgc
      541 agcccttctt tcggcagacg tgcgtgtagc cgagcacgat gccggcgcgg gcgagcgctc
      601 ggcggagcgc ctcctcgagc ggcgtgttcg tcgtcatcat gcggccgtcc ggccccggaa
      661 acaccaggct cgacggagac gacgcgatcg cctcctcgag gtaaggacgg agctcggccg
      721 caatcgggat ggcagcggca cgtccgccct tagtcgtgtc gtggtcgtac gaacggcaga
      781 cgctgatgag gccggtcgtg aagtcgatgt ccgtcttgcg gagccccgct agctcgccct
      841 tccgcatgcc cgtgtagaca gcagtcgcaa agagcgcgcg atggcccggg tacgcagcca
      901 cgagcacgag cggcacctct cccgcgcgca ggaagtcggg aagacgcttc ggcaccttgc
      961 gacggcgaac gtcgcgggcg gggttcggcc cctcccacct ccctgcctgg cgggcgcggg
     1021 taaacgccgc catgacgaac gagcgcaggt ggttcagcga cgaggggctc aaatgcttcg
     1081 acagcgactg caggtgcgtc tcgagcacgc ccgacgtgac ctcggtcaac gtgaagttcg
     1141 cgagcttcgt gtcgaggaag ttcttgcgga cgacgttccg cgttcgctct cccgcggggg
     1201 caccgtccac gtaggtctcg agccaccagc aaaggagctc gccgaacgtg ccgccaccat
     1261 cttcgggagg cagcggctcg aggccgaggc gctgacgttc ggcacgaagc tcgagctcgg
     1321 cggccatgcg cttggcttcc gccttcgtgc gcgctgagga cgcgacggcg cgccagtttc
     1381 cgcgcgcatc cttgtagcgg aggtaaagcg tccccttctt ctcgtagatc gacgccatgg
     1441 ccctacgcgc ccttgcgtag cgcgcggggc cggagcggca aggcgcgcgg acgttgcatg
     1501 tccgagctgc cgtctcgggc gtactgacgg acggcttcgg gctcgaagcg gagcagacct
     1561 ccgatacgaa cgtacggaag gagattcgct tcggcgcggt ggtaaaccca ggagcgggag
     1621 actttcaagt aggcggcgac atcagccgcg gtccacaact cgtccggacg cgcaccgggt
     1681 gcgacctggg agacgcgacg ttcagggtga accatgcttc tcggctctgt cgggagactc
     1741 ccaggcgggc tcgaccttga cgatgtcgaa ggtgcccccc gcgcccgcgt cttcggtcac
     1801 gtgcttcaga aacttctcga gcatcgaggc gatccactcg ggctcgactt cctttgcgtc
     1861 ctcgagctcg acctccagga agacgctgcg cttgccggcg tctttgccgt tcagcttgcg
     1921 atgaacgccg gtcgcgacga cgcgcgcctc gcccgacaag ttgcggaggt acggaacgag
     1981 cattggcttc agcacgttcg cggccagctc aggattatca gcacacctga tagcgatccc
     2041 aattttgatg attgccatgc aggataaggc tgggtggcga ccgattctac cggcctccgt
     2101 cggcatcaag gctggaaccg agaaaggtca cccgctgggt ggctcagcag cttcgccgcg
     2161 tcaccgcggg aacggcgcgc cagatgccac ggcgcccatc tcgaccactc gagcgctgcg
     2221 cgtgagccgg ttgcccggcg tcgcgcttcg cgcatacgag ccaactacat tccaggatag
     2281 tttgtatccg tccgggcaac agcgtcgtga cgatgaggcg tgggatgagg gaagttggcg
     2341 cccatggaga tacaggcacg gtcgcgagtg gcgcgggagg aatgggcgaa gcgggtcgag
     2401 cggtggcgtg agagcgggct gacgagcgcg gagttcgcgg cggagctcgg aatcaacccg
     2461 cgcacgctga cgtactggaa gtgggttctc ggcaaagagg cgctgggcga aaaacgtgag
     2521 tggccgagcc gcaagcgcac ggtgcgagca gggcggccgc caacgagggg cgcggaatcg
     2581 aggccggcgc tgcccgagct cgtcgaggtt catgcggcag cgggcgacgc gcggttcgag
     2641 ctcgagctcg gcgccgggcg gcgcatccgc gtacctgcga gctttgacgc gacggagctg
     2701 cggcggctgc tcgacgtgct ggaggcgccg tgattcctgc tgccgtgcgg atcttcgtga
     2761 gcaccgagcc aatcgacatg cggcaaggct tcgacaggct cgcgcaggcg gtgcgagcgc
     2821 gactcggcga agagccacaa agcgggtcgc tgttcgtgtt cgccaaccgc ggagcgacgc
     2881 gcctgaaggt gctttggttc gacagcaacg gctattgcct actctacaag cggttgcacc
     2941 gcgcagtgtt cgagttgcct ccgtcgggtg gaggcgcggc cgtgcaagtc gacgcacgac
     3001 gactcgccga gctcctgcgc ggcgccgctc gcgagcgcgc gcgaaaaagt cagcatcgaa
     3061 gtgctgctgc agcttgacaa gcgatcttcg aatcggcatt cgttctcgtc gtgagcgaag
     3121 ccgctctcgc cgacgatccc gagcagctct ccgctgaaac ggcgctcgct gagttgctcg
     3181 cgcgcgtcgc aaccgtcgag cacgagcgcg acgagttcaa gcacgagcgc gacgaataca
     3241 agaagctcta tctgtttctg cgcgaagaaa acgagcgact caagcgcggg ctcatcgggc
     3301 agaaggcgga gcggctgccg cgaaacgacg cccagctctc gctcgccgtg ctcggcctga
     3361 tgctcgcaaa cggcgaaaat cccggcgctc cgcccgagga cgagcccgag cagcagatcc
     3421 cggctcacac gcgcaaagtc ccgaagcgaa ggacgctgcc cgccgactgg ccccgcgtca
     3481 ccatcgagct tgtcccgccc gaggtcgagc gcgagggcct cgacgcgttc gagatcatcg
     3541 gtgaagagcg acgcggtgtg ctcgagcgcc gtcccgcttc gtgcgtcgtc gtcgaggtcg
     3601 t
//
LOCUS       DASTKS010001168         6001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_631001, whole genome shotgun sequence.
ACCESSION   DASTKS010001168 DASTKS010000000
VERSION     DASTKS010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631001"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /gene="cysN"
                     /locus_tag="VFQ35_24870"
     CDS             complement(<1..1598)
                     /gene="cysN"
                     /locus_tag="VFQ35_24870"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HET9933969.1"
                     /translation="MQETELIRQDILGYLEQHQQKELLRFVTVGSVDDGKSTLIGRLL
                     HDTHGVYEDQLKAVKRASTRAGLEIDFSLFTDGLKAEREQGITIDVAYRYFSTAKRKF
                     IIADTPGHVQYTRNMATGASTANVAIILIDARLGVLQQSRRHAYIASLLGIPHLTVCI
                     NKMDLEGYSQAVFDKIREAFQAFCAQLNFKAVTFIPVSALAGDNVVHRSQSMGWYKGP
                     TLLEHLEQVPVAEDINLNDFRFPVQWVMRPDLDYRGFSGTISSGIVKQGDTIQVLPSG
                     KTTRIVAIDVDKGSIPQAHAPMAVTLRLAEEIDISRGDMLVHPSNLPTSSQRFDAMLV
                     WMSERPLDRQKSYLLKHTTQRVRAEVAQVAFTMDLETLKEQPATRLELNDIGRVTITC
                     HRPLFFDPYRNNRSTGAFILIDSLTNNTVAAGMILEGEAAPENRASQRPGALVGTPHS
                     QVSHEERAERLGQKGCTVWLTGLPASGKSEIAYALERRLFDIKRLAMVIDPDDGLSTG
                     VQPDGSSPWQTPELARRATDAGLI"
     gene            complement(1698..2633)
                     /gene="cysD"
                     /locus_tag="VFQ35_24875"
     CDS             complement(1698..2633)
                     /gene="cysD"
                     /locus_tag="VFQ35_24875"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET9933970.1"
                     /translation="MLAPADVDKAVDLRLTHLRQLESESIHILREVVAEFERPVMLYS
                     IGKDSSVMVHLAKKAFHRGKLPFPLLHIDTTWKFKGMIEFRDRFCRENGFDLRVHTNK
                     QGVEEGINPFDHGASKYTTIMKTHALLQALHDGGYDAAFGGARRDEERSRAKERIYSF
                     RDRYGQWDPKNQRPELWNLYNAKINKGESIRAFPLSNWTELDIWLYIYLENIPIVPLY
                     FAAKRPVVERDGTLVMVDDERMRLEPGEKPREELVRFRTLGCYPLTGAIPSTATTLPE
                     IIREMLLTKYSERQGRLIDHDEEASMEVKKREGYF"
     gene            complement(2832..3596)
                     /gene="aat"
                     /locus_tag="VFQ35_24880"
     CDS             complement(2832..3596)
                     /gene="aat"
                     /locus_tag="VFQ35_24880"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET9933971.1"
                     /translation="MNLDARRLRRGILMAVAHSRPPQILLPGTSPRFPDPNRSDARGL
                     VAAGGELSPEWLLRAYEHGIFPWYDEGLPILWWSPDPRTLMDPSTLHVSRSMRRFIRS
                     TTLSLTHNRAFTRVMLEASREREGGTWILPEMVDAYTRLHELGHAHSIEVWQGAELVG
                     GLYGVQRGALFAAESMFHRVTNASKLALIATVQTLFAAGIELFDVQFTTSHLSSLGAH
                     EISRRDYLGRVARAIRVPVSLERLEVRALPLPPNLD"
     gene            3746..4000
                     /locus_tag="VFQ35_24885"
     CDS             3746..4000
                     /locus_tag="VFQ35_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933972.1"
                     /translation="MARRGDDRKAMIAAREACFAEPKDARLWALYGAWCFRARHREEA
                     LHAFGQSLWLRQRAKDERRAQVLRKVIEQVELGADTLHAA"
     gene            complement(4004..4525)
                     /locus_tag="VFQ35_24890"
     CDS             complement(4004..4525)
                     /locus_tag="VFQ35_24890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933973.1"
                     /translation="MQTQLIAALLALSFGSACASTSGRAWVRAPEDANLETDVASVDL
                     APLPAGASSELSDSKSSDETGDVPRPRLAHVISLGESEAAPRSAQPSGAPLTSATPGV
                     VVNIVNYGSSAYAGGYGYPLSYRTATSGAPVVHSSPTVGTPSLGGDWRPAPSYGPSFP
                     WHTGPAPAWERSR"
     gene            4743..5636
                     /locus_tag="VFQ35_24895"
     CDS             4743..5636
                     /locus_tag="VFQ35_24895"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9933974.1"
                     /translation="MQSTAKRPLFELVRHDHDLGIVFRDVTARALQYPWHFHPELELT
                     HVVKGSGVRYVGDSIEQFEDDDLCLIGASTPHCWLTEVGRSDPVHARVIQFLPESVTT
                     SLADSITFRPLSTLFARARRGLLIRGSARQRTLEAMRKLFVGPQRPLDRFVGLLAILA
                     DLSDSDELAELSLGNGVLPDDKGSAEVASRLLGYIHENAANPNLSFSSVARACGMSRA
                     TLGRAFPRLFGKTFVKYLSEVRVTQACTLLTETTRSVSEIALATGFGSLSNFNRQFRT
                     LKQTTPLRYRRSTAVRDAVRC"
BASE COUNT         1038 a         1846 c         2030 g         1087 t
ORIGIN      
        1 cagatgaggc cggcgtcggt ggcgcggcgc gcgagctccg gtgtttgcca cgggctcgag
       61 ccgtccggtt ggacgccggt ggagaggccg tcgtcggggt cgatgaccat ggcgagacgc
      121 ttgatgtcga agaggcggcg ctcgagagcg taagcgatct cgctcttgcc ggacgcgggg
      181 agcccggtga gccaaacggt gcagcccttt tggccgaggc gctcggcgcg ttcttcgtgg
      241 ctgacttgcg aatggggcgt gccgacgagc gcgccgggac gctgcgaagc gcggttctcc
      301 ggggcggctt caccttccag gatcatgccc gcggcgacgg tgttgttcgt cagcgagtcg
      361 atcaggatga aggcgccggt gctgcggttg ttgcggtaag gatcgaagaa cagcgggcgg
      421 tggcacgtga tcgtgacgcg accgatgtcg ttgagctcga ggcgcgtcgc cggctgctcc
      481 ttcagcgtct cgaggtccat cgtgaaggcg acttgcgcca cttcggcgcg gacgcgctga
      541 gtggtgtgct tcaagaggta gctcttctga cggtcgaggg gacgttcgct catccacacg
      601 agcatcgcgt cgaagcgctg actcgaggtc ggcaagttgc tcggatggac gagcatgtcg
      661 ccgcggctga tgtcgatctc ctcggcgagc cgcagcgtga ccgccatcgg ggcgtgcgct
      721 tgtgggatgg agcccttgtc gacatcgatc gcgacgatgc gagtagtctt gccggagggc
      781 agcacctgga tggtgtcgcc ttgcttgacg atgccggacg aaatcgtgcc ggagaagccg
      841 cggtagtcga gatccggccg catcacccac tgcacgggga agcggaagtc gttgaggttg
      901 atgtcctcgg ccaccggcac ctgctcgagg tgctcgagca acgtcggacc tttgtaccaa
      961 cccatgctct ggctgcggtg cacgacgttg tcgcccgcga gcgcgctcac cgggatgaac
     1021 gtgacggcct tgaaattgag ctgggcgcag aaggcttgga acgcctcgcg gatcttgtcg
     1081 aagacggctt gcgagtagcc ttcgagatcc atcttgttga tgcaaacggt caggtgcggg
     1141 atgccgagca gggacgcgat gtaggcgtgg cgacgcgact gctggagcac gccgagacgc
     1201 gcgtcgatca gaatgatggc gacgttcgcg gtggaagcac cggtcgccat gttgcgcgtg
     1261 tactgaacgt ggccgggcgt gtccgcgatg atgaacttgc gcttcgccgt ggagaagtag
     1321 cggtaggcaa cgtcgatcgt gatgccctgc tcgcgctccg ctttgaggcc gtcggtgaac
     1381 agcgaaaagt cgatctcgag ccccgcgcgc gtcgaggcgc gcttcacggc cttcagctgg
     1441 tcttcgtaca cgccgtgcgt gtcgtgcagc aaacgcccga tgagcgtcga cttaccgtcg
     1501 tcgacggagc cgacggtgac gaagcgcagc agctcctttt gctggtgctg ttcgagatag
     1561 ccaaggatgt cctgacggat gagctcggtt tcctgcataa tcttcgtgcc tggctcagga
     1621 gagacgactc tcttcgacga ttccaaaact cgaggggagg ctccagtcgc ggctcttgga
     1681 gaggagccgc gctcccgtca gaagtaccct tcgcgctttt tgacttccat cgacgcttcc
     1741 tcgtcgtggt cgatgaggcg cccctgccgc tcggagtact tggtgagcag catttctcgg
     1801 atgatttcgg gaagggtggt ggcagtggac gggatcgcgc cggtgagcgg atagcaaccg
     1861 agcgtgcgga agcggaccag ctcctcgcgc ggcttctcgc cgggctcgag gcgcatgcgc
     1921 tcgtcgtcga ccatgaccag cgttccgtcg cgttcgacga ccggtcgttt ggcggcgaag
     1981 tagagcggca cgatcgggat gttctcgagg tagatgtaga gccagatgtc gagctcggtc
     2041 cagttcgaca acgggaaggc gcggatgctc tcgcccttgt tgatcttggc gttgtagagg
     2101 ttccagagct cggggcgctg gttcttcgga tcccattgcc cgtagcgatc gcggaacgag
     2161 tagatgcgct ctttcgcgcg cgagcgctct tcgtcgcgac gcgcgccgcc gaacgccgcg
     2221 tcatagccgc cgtcgtgcag cgcttgcagc agcgcgtgcg tcttcatgat cgtcgtgtac
     2281 ttcgacgcgc cgtggtcgaa gggattgatg ccctcttcga cgccctgttt gttggtgtgc
     2341 acgcgcaggt cgaagccgtt ctcgcgacag aaccggtcgc ggaactcgat catccccttg
     2401 aacttccagg tggtgtcgat gtgaagcagt gggaacggca gcttgccgcg atggaacgcc
     2461 ttcttcgcga ggtgcaccat caccgacgag tctttgccga tcgagtagag catcacgggc
     2521 cgctcgaact cggcgaccac ctctcgcaaa atgtggatgc tctccgactc cagctgccgc
     2581 aggtgggtca gacgtaggtc gacggcttta tcgacatccg caggggccaa catcggcggg
     2641 catataacac caaatgcatc ggaattaggg gattctgcct aaattgtcgg atttaaagga
     2701 gaacaatgcg acaggcggcc cgttagccgg cgctacaccc ccgctttggc ctgcggagcg
     2761 gtcgcctgcc ggtgaactcc tggcgcgcgg accctccgac accgggtcct ccaatcccgc
     2821 cgcccgaagg ttcagtcgag attggggggg agcggaagag cgcgcacctc gagccgctcg
     2881 aggctcaccg gaacgcgaat ggctcgtgca acgcgcccaa ggtagtcgcg ccgcgagatt
     2941 tcgtgggcgc cgagcgaact caggtgtgaa gtcgtgaatt ggacgtcgaa gagctcgata
     3001 cccgccgcaa acagcgtttg caccgtcgct atcaaggcga gcttcgaggc attcgtcacc
     3061 cgatgaaaca tgctctccgc ggcgaatagc gccccccgct gtacgccgta caacccgccc
     3121 acgagttcgg cgccctgcca cacctcgatg ctgtgcgcgt gaccaagctc gtggagacgc
     3181 gtgtaggcat cgaccatctc cggcaagatc caggtgcctc cctcgcgttc gcgcgacgct
     3241 tcgagcatga cgcgagtgaa ggcgcggttg tgcgtgagcg atagcgtcgt gctgcgaatg
     3301 aagcgacgca tgctgcgcga cacgtgcagc gtgctcggat ccatcagtgt gcgcggatcc
     3361 gggctccacc agaggatcgg cagcccctcg tcgtaccagg ggaagatgcc gtgctcgtag
     3421 gcgcggagga gccactcggg ggatagctca ccgcccgccg cgacgagccc gcgcgcgtca
     3481 ctgcggttcg gatcgggaaa gcgcggagac gtgccgggta agaggatctg gggaggtcgc
     3541 gagtgcgcca cggccatcaa gataccgcga cgtaggcggc gagcatcgag gttcacacct
     3601 aactcgtctc gaacgtcgat tttcgagaca gaacccgaaa gtttgctccc ggactggccg
     3661 aactaccgag cgggaaatgt cgaagaagcg cagaaaagct cctgcagaga cccgcgaaac
     3721 ggtcctgctt cgacgtgccc atcggatggc tcggcgtggc gatgaccgca aggcgatgat
     3781 cgccgctcgg gaggcatgct tcgcggagcc gaaggacgcg cgcttgtggg cgctctatgg
     3841 cgcttggtgc ttccgcgcac gccatcgcga agaagcgctg cacgcgttcg ggcagagcct
     3901 atggcttcgc caacgcgcga aggacgagcg acgcgcgcag gtactacgta aggtcatcga
     3961 gcaggtcgag ctcggcgcgg acacgctgca cgcggcgtga tcgtcagcgc gaccgttccc
     4021 acgccggcgc aggcccggtg tgccacggaa agctcggacc gtagctcggc gcgggcctcc
     4081 agtcgccccc gagcgaaggc gttccgacgg tcggactcga gtgcacgacg ggagcgccgc
     4141 tcgtagccgt gcggtacgag agcggataac cgtagccgcc agcgtaggcg gagctgccgt
     4201 agttcacgat gttgacgacg acgccgggcg tcgcggacgt cagtggagcg ccgctcggct
     4261 gagcggagcg tggagcggct tcactctcgc cgagggagat cacgtgtgcg agccgcggac
     4321 gcggcacgtc tccggtctcg tcggacgact tcgaatccga aagttcggat gaggcgccgg
     4381 caggcagcgg cgcaaggtcc acactggcga cgtcggtttc gaggttggcg tcttcggggg
     4441 cgcgcaccca agcgcgaccg ctggtcgaag cgcaggccga cccgaaagag agggcgagca
     4501 gcgctgcgat cagctgggtt tgcatcggaa agaagcttgc cacgcgcagg cccgcccggc
     4561 ccgttacggc gtcgaaaccg cgcaaacgcc gggctcgcgc ggtcgagctc ggttgagttt
     4621 tttccgcctg gttgcagagc cgcacccgtc gagcggccag gtgttctcgt tgtggaaccg
     4681 cttccagcga ctcgacggat cgcctcgacg aagtcgcgct ctggagctag tctgttcgcg
     4741 ccatgcagag cacagcgaag cgcccgctgt tcgagctagt gcgtcacgat cacgatctcg
     4801 gcatcgtctt ccgcgatgtc accgctcgcg cactccaata tccctggcat ttccacccgg
     4861 agctcgagct cacgcacgtc gtgaaaggct ccggtgtacg ttatgtcgga gacagcatcg
     4921 agcaattcga agacgacgac ctttgcttga tcggcgcgag cacgccgcat tgctggctca
     4981 ccgaggtggg ccgcagcgat ccagtgcacg cgcgcgtcat tcaattcctg ccggagtcgg
     5041 tcacgacctc actcgccgac tcgatcactt tccgcccgct ctcgacgctc ttcgcccgcg
     5101 cccgaagagg gcttttgatc cgcgggagtg ctcggcagcg caccctcgag gcgatgcgca
     5161 agctcttcgt cgggccccag cggccgctcg atcgcttcgt gggtctgctc gccatcttgg
     5221 ccgacttgtc cgacagcgac gaactcgcgg agttgtcgct cggcaacggc gtgctgcccg
     5281 acgacaaggg ttcggcagag gtcgctagcc gcttgctcgg atacattcac gagaacgcgg
     5341 cgaacccgaa cctctctttt tcgagtgtgg cccgcgcctg tggcatgtcg cgcgcaacgc
     5401 tcggtcgcgc cttcccccgc ttgttcggaa agactttcgt caagtacctg tccgaggttc
     5461 gcgtcaccca agcctgcacg ctgctgacgg aaacgactcg cagcgtgagt gagatcgcgc
     5521 ttgcgactgg ctttggcagc ctctcgaact tcaaccgcca gtttcgcacg ttgaagcaga
     5581 cgactcccct ccgctaccgt cgctccacgg ccgtgcgcga cgccgtccgc tgctgagttc
     5641 ccaaagaact tgtcgctcgg atgcgggctc gacgagccca ccgcgagtag gtcggacgag
     5701 aaacgccacg gacttcgatc ataggggcgt cgcgatcgtc tagcaacgat catggctcga
     5761 gcgcttcgcc ggccacaccc taatcgccgg acgaagcaat cgagacgcgg acacgacgta
     5821 cgcgggctcg acgggttgcg ttcgggattg gagggtccaa ccatgatttc cgggcgggcg
     5881 gagactccga gcaccgtgag acggggagca actaggacac gtcgccgctg gacggcgatg
     5941 atcacgagcg tcgcttgcgt aacgtcgctt gctctcgtcg tgagctgcgg caacgccgaa
     6001 g
//
LOCUS       DASTKS010001169         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1748736, whole genome shotgun sequence.
ACCESSION   DASTKS010001169 DASTKS010000000
VERSION     DASTKS010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748736"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFQ35_24900"
     CDS             <1..2083
                     /locus_tag="VFQ35_24900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933975.1"
                     /translation="LDELANASRAEAPRRAAWLLEAAEASLAASSFELALARAVRAAR
                     LDSRSLAAQLLARSLEYKQRGPGSADDAEITLNELNHAEAEEPSVEQIELAAFLRAEA
                     LDVLSRFDEARGELERAESQTGPRPLTSLARGERQSRAGDLAAALPNFDAALGGDLRG
                     LRRRGRVAWHAAEVAFALGDLDRTESYLQISAEDPETREQANARIRELFSERVSVQPP
                     PIPSDAPAPMLVRRPSQPAPRASNRPSQPVPAERALASLGRYSGKPPSEGAEERIAVP
                     SRISVEPAANAPAKAPGRYSVRVEAPPSEPPPAPAAPVEGAADSSSKLLRFDADEASL
                     YRSLAEGVVEAGHELILKLEGRSDRTHDLVAVCRRLVLAQPGDPSALDRLYRATLADK
                     DFAYARAIEHVVGVLDPGAPYVEPPPLSEQSEQPDAVRSLLLRDHGSRAFEALSVVWE
                     GAERVFRRDPSTYGVTGLERVPLTAPMPLARTYSGVARSLGMLRTPLFQRRSAGPITV
                     SLALLSPPAVVLSGDVRQESPELAYQLGATLLAASPSFVLLLGSAESQARAILKGLAF
                     AFGPPQSGGIGPGAVPSLAEVLWESIPARLQRRLRELCDSPAALEYDVAVRLARSAAR
                     RAGLFASGHLGVALTAACAEEGIPESALSSASRVSALAAQNSSVRSLLALATSPEYAQ
                     VRWWLGRGGGR"
     gene            2154..>2507
                     /locus_tag="VFQ35_24905"
     CDS             2154..>2507
                     /locus_tag="VFQ35_24905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933976.1"
                     /translation="MTISAKTLFEAARVAAGRMRGLFATRLSHRAPEKTPPSSGKMRL
                     ARSSLLCLCAAPSLSGCIVADPPEYQDPGRTPPFLNLALATPRVLDIQVIDLTTNPAN
                     ANFSVNVPVRSDDKDT"
BASE COUNT          351 a          936 c          808 g          412 t
ORIGIN      
        1 gctcgacgag ctcgccaacg cgtcgcgggc cgaggcgcct cgacgcgcgg cctggctcct
       61 cgaggcggcc gaagcctcgc tcgcggcttc gagcttcgag ctcgcgctag cccgcgccgt
      121 gcgcgcggct cgtctcgact cgcgctcgtt ggcggcgcag ctgctcgcgc gctcgctcga
      181 gtacaagcaa cgcggcccag gctcggccga cgacgccgaa atcaccctga acgagctcaa
      241 ccacgccgag gccgaggagc ccagcgtcga gcaaatcgag ctcgccgcgt ttcttcgcgc
      301 ggaggcgctc gacgtgctct ctcggttcga cgaggcgcgc ggcgaactcg aacgcgccga
      361 gtcgcagacc ggtccgcgcc cactgacgtc cctcgcacgc ggcgagcggc aaagccgggc
      421 aggggacttg gcggctgcgc tgcccaactt cgacgcagcg ctcggcggcg acctccgggg
      481 gctccgacgc cgcggccgtg tcgcttggca cgccgccgaa gtagcgttcg ctctcggcga
      541 tctcgatcgc acggagagtt acctccagat cagcgccgag gatcccgaaa cccgcgaaca
      601 ggcgaacgct cggattcgtg agctcttctc ggagcgcgtc agcgtccagc cacctcccat
      661 accctccgac gctccggccc cgatgctcgt ccgtcggccg tcccagcccg cgccgcgtgc
      721 ctcgaaccgc ccttctcagc cggttcccgc cgagcgggcg ctcgcatcgc tcggtcggta
      781 ctcgggaaag ccgccgagcg aaggcgccga ggagcgtatc gcggtgcctt cgcgaatcag
      841 cgtcgagcca gcggcgaacg cgcccgccaa ggcgccgggg cgctactcgg tgcgagtcga
      901 agcgcctcca tcggaaccgc ccccggcgcc ggccgcaccc gtagaaggcg ccgcggactc
      961 ctcgtcgaag ctgcttcgtt tcgacgccga cgaggcgtcg ctctaccgtt cgctcgccga
     1021 gggcgtcgtc gaggccggtc acgagctcat cctcaagctc gaaggtcgct cggaccgaac
     1081 ccacgacttg gtcgccgtat gtcgccgcct cgtcttggcg caaccgggcg atccctccgc
     1141 gctcgatcgc ttgtaccgcg cgaccctcgc cgacaaagac ttcgcgtacg ctcgcgccat
     1201 cgaacacgtc gtcggggtgc tcgatcccgg cgctccttac gtcgaaccgc cgccgctctc
     1261 ggagcaatcg gagcaaccgg atgcagttcg ctcgctcttg ctgcgcgatc acggctcgcg
     1321 tgccttcgag gctctttcgg tggtctggga gggcgccgag cgtgtcttcc gccgcgaccc
     1381 ctcgacgtat ggggtaactg gcctcgagcg agtgccgttg accgcgccga tgccgctcgc
     1441 ccgcacctac tcgggcgtcg cccgctcgct cggcatgctc cggaccccct tgttccagcg
     1501 gcgcagcgcg ggccccatca cggtgagtct cgcgctcctg tctccgcccg cggtggtgct
     1561 ctccggtgac gtcaggcaag aaagcccgga gctcgcttac cagcttggcg caacgctcct
     1621 cgccgcgagc ccgagcttcg tgctgttgct cggttctgca gagtcgcagg cgcgagccat
     1681 tctcaagggc ctcgcctttg ccttcggtcc tccccaaagc gggggcattg gtccgggcgc
     1741 cgttccgagc ttggccgaag tcttgtggga gagcattccg gcgcgcttgc agcgccgttt
     1801 gcgcgaactc tgcgattcgc ccgccgcgct cgagtacgac gtcgcagtgc ggctagcccg
     1861 cagcgctgcc cggcgcgcag ggctcttcgc gagtgggcac ctgggcgtcg cgctcactgc
     1921 cgcatgcgcc gaggagggca tcccagagtc agcgctctcg tccgccagtc gcgtgagcgc
     1981 cctggccgcg cagaactctt cggtgcgctc gctgctcgcc ctcgcaacga gcccagaata
     2041 cgcccaagta cgctggtggc tcgggcgcgg tggggggcgc tgagccccgc tttgacgctt
     2101 ttcgcgggat gcgccacata cgccttggca ccgagccgga tcgggacccc accatgacta
     2161 tctccgcgaa gacgctattc gaggccgccc gcgttgccgc tggccgcatg cgaggtcttt
     2221 tcgcgacacg gctgtcacac cgggcgccgg agaagacgcc gccgtcgtcg ggaaaaatgc
     2281 ggttggcacg ctcgtcgctc ctgtgtctct gcgccgcgcc gagtctctct ggctgcatcg
     2341 tggcagatcc tcccgaatac caggaccccg ggcgcacgcc gccgttcctc aatttggcgc
     2401 tcgcaactcc ccgtgtgttg gacattcagg tgatcgatct cacaaccaat ccggcgaacg
     2461 ccaacttcag tgtgaacgtg cccgtgcgat ccgacgacaa agacacc
//
LOCUS       DASTKS010001170         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_407564, whole genome shotgun sequence.
ACCESSION   DASTKS010001170 DASTKS010000000
VERSION     DASTKS010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407564"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFQ35_24910"
     CDS             complement(<1..789)
                     /locus_tag="VFQ35_24910"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9933977.1"
                     /translation="MSPADTAILENFVLPRYLARFGELALDMLVSGPAARILHVGCRT
                     GYPDRELLERVPEAEILGVDASFAAIEAARERGRNARPGALEYRVSESLPGDLEPGIF
                     THALCLHPLVSGRERAELWSALRWLLCEGGQALVALPLRGSYAEVIDLLREYALKYDD
                     SEFARDLEDHVGRCLSLETLSDEFEEAGFDDVDVEVRTFTLPFESGRSFAEDPVSRLF
                     VLPEIHTWLEREDLERPLSYVRDAIDKYWSEGKLELTVSVGCASA"
     gene            1065..1715
                     /locus_tag="VFQ35_24915"
     CDS             1065..1715
                     /locus_tag="VFQ35_24915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9933978.1"
                     /translation="MSLPDLAGAEAPEVEQNEHHDKQGRLVRYEHIPRRPERPADYRR
                     YLLPIALDEKHAGIGSGYDLDLPDAEQRRGSGLKAVGHGGLDLAAARGAPVHGVRLEH
                     QRGDAEVLYVGELFGTTVVTLHSVAEGGALRDYLIVHGHLERANSTLHKGDGVTPDTT
                     LGFVGDTGSPGIVHLHFEIRRVRDGVDVRELGPRELVHNAKTIACDPRNVLELGAN"
     gene            complement(1760..2449)
                     /locus_tag="VFQ35_24920"
     CDS             complement(1760..2449)
                     /locus_tag="VFQ35_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9933979.1"
                     /translation="MESKRTILVVDDEPHIVLGLKDALEFEGFRVLTAATGKEGAQLA
                     QREHPNAILLDLMLPDMNGYQVCEDIRRWDAIVPILMLTAKSQEADKIRGLDAGADDY
                     VTKPFSVGELVARLRALFRRTQRANESPSFRIGTVSINVSSHTLQREGQPAETLSFYE
                     VELLRLLHERLGQPVSREEILNKIWGIEANPSNRTIDNFIVKLRKKIEMKPDKPEHIL
                     TVYGFGYKLVE"
     gene            complement(2565..>3227)
                     /locus_tag="VFQ35_24925"
     CDS             complement(2565..>3227)
                     /locus_tag="VFQ35_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933980.1"
                     /translation="PGVRELQVLKDFGAKGTQNSIVASICPKTLTVQKTDPSYGYNPA
                     VSAIIDRLKEALKGKCLPRQLIPDENNDNKVPCAVIEATPPGAPCTCDAAGRSPANEG
                     IRPAVLKQLTENDTCGPKSPNQTPCEQFCLCEIQQYSGGDLDTCQTAPQPPGNITPGY
                     CYVDPSTADEGSQKRAAQEAIVASCPATQKRLLRFAGENTPAKGAIAMIACLGANVQD
                     KK"
BASE COUNT          571 a         1058 c         1031 g          567 t
ORIGIN      
        1 cgcgctcgca cagcctaccg agacggtgag ctcgagcttg ccctcggacc aatacttatc
       61 gatcgcgtcg cgcacgtacg aaaggggccg ctccaagtct tctcgctcga gccaagtgtg
      121 gatctcgggc aagacgaaga ggcggctcac gggatcttcc gcgaacgagc ggccgctctc
      181 gaacggcaac gtgaaggtgc gcacttcgac gtcgacgtca tcgaaacccg cctcctcgaa
      241 ctcgtcggag agggtctcga gcgataggca acgccccacg tgatcctcga ggtcacgcgc
      301 gaactccgag tcgtcgtact tcagcgcata ctcgcgcagc agatcgatca cctccgcgta
      361 cgacccgcgc agcggtagag cgacgagtgc ctgaccgcct tcgcacagga gccagcggag
      421 cgcgctccag agctctgctc gctcgcggcc cgagacgagc ggatgcaagc acagcgcgtg
      481 agtgaagatc cccggctcga gatcacctgg cagcgactcg gagacgcggt actcgagcgc
      541 gccgggacga gcgttgcgac cgcgctcgcg cgcggcctcg atggccgcaa acgacgcgtc
      601 cacacccaga atttcggcct cggggacccg ctctagcaat tcgcgatccg ggtagccggt
      661 gcgacagccg acatggagga tgcgcgcggc aggccccgaa acgagcatgt cgagcgcaag
      721 ttcgccgaac cgcgcgaggt acctcggcaa aacgaagttc tcgaggatcg ctgtatcggc
      781 ggggctcaat ttttctcgag caagcacgtc cctctcggct atcaattcgg gagcttcgga
      841 tcaatcctgg aatcgatgag gccgagcgcg cgttcagatg ccccatgcgg ccgatagttc
      901 ggatagctct ttgggccagc gtgacgctcg cgctggttcg gtgggcagag cgtgacgagg
      961 cccacgcggc agcgcccgcg accgcgccga gctccgccac acgacccgga ttccttgccc
     1021 tatgcccggc cgcgactctg cctgaccacg gctcctgcat cccggtgtct ttgccggacc
     1081 tcgccggcgc cgaagcaccc gaggtcgagc aaaacgagca ccacgacaag caagggcggc
     1141 tggtgcggta cgagcacatt ccgcgtcgcc cggagcgccc cgccgattac cggcgctacc
     1201 tgcttcccat agcgctcgac gagaaacacg caggcatcgg cagcggctac gatctcgact
     1261 tgcccgacgc ggaacaacgg cggggcagcg ggctcaaggc cgttggtcac ggcgggctcg
     1321 acctcgccgc cgcgcggggc gcgccggttc acggagtccg cctcgaacat caacgaggcg
     1381 atgccgaggt cctgtacgtg ggggagctat tcgggaccac ggtggtaact ctgcattcgg
     1441 tggctgaagg tggcgctctg cgcgactacc tcatcgtgca tgggcacctc gagcgagcca
     1501 actcgacgct gcacaagggg gacggcgtca ccccggacac gactctcggc ttcgtcggag
     1561 acacgggcag ccccggcatc gtgcacctcc acttcgagat tcggcgcgtg cgcgacggcg
     1621 tcgacgtccg agagctcggg cctcgcgagc tggttcacaa cgccaagacg atcgcgtgcg
     1681 acccacgcaa cgtgctcgag ctcggcgcca actgacgctg cgcaatcgcg gcttggcgtg
     1741 ccttcacgaa ccgagcgcgc tactcgacga gcttgtagcc aaaaccgtac acggtgagga
     1801 tgtgctcggg cttatcgggc ttcatttcga tctttttgcg cagcttgacg atgaagttgt
     1861 cgatcgtgcg attgctgggg ttcgcctcga tgccccaaat tttgttgagg atttcctcac
     1921 ggctcacggg ctgccccaat cgctcgtgca acaagcgcaa aagctcgact tcgtaaaagg
     1981 agagagtctc cgcgggttgc ccctcacgct gcagcgtgtg gctcgagacg ttgatgctga
     2041 cggtcccgat ccgaaagctg ggagactcgt tcgctcgctg cgtccggcga aagagcgcac
     2101 gcagccgggc cacgagctcg ccgacgctga acggcttggt cacgtaatcg tcggcgcccg
     2161 cgtcgagacc acggatcttg tcggcctcct ggctcttggc ggtgagcatc aagatcggca
     2221 cgatcgcgtc ccaacggcgg atgtcttcgc aaacctgata accgttcatg tccggcagca
     2281 tcaggtcgag caggatcgcg tttggatgct cgcgctgcgc gagctgtgcg ccttccttgc
     2341 ccgtcgccgc ggtgagcact cgaaaccctt cgaattcgag agcgtctttc aacccgagca
     2401 cgatgtgcgg ctcgtcatcg accacgagga tggtgcgctt cgactccatc gcagccgcac
     2461 cgtggcgcga cgtgccgcgc gatgcaaggg ctacaaaacg ccgacgccgc cgctccgaag
     2521 aacgacggcg tcgtcgatgt gcccgccttc gggctaccgt tcggtcactt cttgtcctgc
     2581 acgttcgcac ccaagcacgc gatcatcgcg atggcgccct tggcgggcgt gttctcaccc
     2641 gcgaagcgca agaggcgctt ctgcgtcgcg gggcagctag ccacgatcgc ttcctgcgcg
     2701 gcgcgcttct gggagccttc gtccgcggtc gagggatcga cgtagcagta tccgggggtg
     2761 atgttgccgg gcggctgagg tgccgtttgg caggtatcca gatccccgcc cgagtactgc
     2821 tggatttcgc acaggcagaa ctgctcgcac ggcgtctgat tgggcgactt tggtccgcag
     2881 gtgtcgttct cggtgagctg cttgagcacc gcaggacgaa tcccctcgtt tgccggactg
     2941 cgaccggccg cgtcgcaagt gcaaggtgca ccgggcggcg tcgcctcgat gacggcgcac
     3001 ggaaccttgt tgtcgttgtt ctcatccggg atgagctgac gcggaaggca cttgcccttc
     3061 agagcttcct tcaagcggtc gataatcgcc gaaacggcag ggttatagcc gtagctcggg
     3121 tccgtcttct gcacggtgag cgtcttcggg caaatcgaag cgacgatcga gttctgagta
     3181 cccttcgcgc cgaagtcctt cagaacctgg agctcacgaa cgcctgg
//
LOCUS       DASTKS010001171         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_631514, whole genome shotgun sequence.
ACCESSION   DASTKS010001171 DASTKS010000000
VERSION     DASTKS010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631514"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..1136)
                     /locus_tag="VFQ35_24930"
     CDS             complement(405..1136)
                     /locus_tag="VFQ35_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933981.1"
                     /translation="MKSEVNAILERLEASERLVNRLESVAARLTDSPLGGEERGWLAA
                     AARRLSASIPAVRAAIETSLDLPELKALRAERLTGLEQAWVGSVRELFGELEKRLGAN
                     SPLIEALFPHLRFDRIERPGAAQRAFRAEVSTRRTSTYIQRMSADPEYPFLAGLLEPV
                     ERAERALAAFESSAPPGEEELLRLRAEVVEAGQGLSRAQRQARALAEAALIDAPELFT
                     ELGFDAPARRRSARPEASVNDDSES"
     gene            complement(1207..>2153)
                     /locus_tag="VFQ35_24935"
     CDS             complement(1207..>2153)
                     /locus_tag="VFQ35_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933982.1"
                     /translation="PSLPEDAPATPIEPNTHDTGRLQPLGEQVVIATRTGRHSVIAYR
                     YDFRDAHFEPEMRALVVPPVSMYGVGLTGGSHDTFGQLDTQFAVHLAVDDASTIYVAV
                     QHPHDGSANLVKAHKKVFGEDLVGDPDSLDIYVTRLALDGTRLGTSVVGTPELDELYG
                     LRAGKSAAYVAGRKEYWNDAGTGFDALAAEVDGKTGAVRVTELDVDRSDIAFDVAPLS
                     DAELLIVGASGYSQNPHGASVSEESQGFAYVLAEGGAKRALLVPNRQRHNEVRSLARD
                     ADGRWFSAGMLDGPGTHSGDADATLVRADGFLTELGVD"
BASE COUNT          364 a          717 c          704 g          368 t
ORIGIN      
        1 gcagccgagg tggggaccac ccctttcatg ccgaccttgc ggtgagcgaa agtgacgaag
       61 ctccacctgc tcgcgacgta cgacgaatcc tgagcgggcg tgctgtcagg gttcagaact
      121 cgaaactagt cgtcagcccg ccggttttgg ggctggggcc ccgaaaccca cacaaacgaa
      181 gcatcggaac gaactctttc gcagccgagg tggggaccac ccctttcatg ccgaccttgc
      241 ggtgagcgaa agtgacgaag ctccacctgc tcgcgacgta cgacgaatcc tgagcgggcg
      301 cgctgtcagg gttcagaact cgaaaccagt cgtcatcgcg ccggttttgg ggctggggcc
      361 ccgaaaccca cacaaacgaa gcatcggaac gaactctttc gcagtcagga ttccgagtcg
      421 tcgttcacgg aggcttcagg ccgtgcgctt cgccgccgcg ccggtgcgtc gaagccgagc
      481 tcggtgaata gctcgggcgc gtctatcagt gcggcttcgg cgagagcgcg agcctgtcgc
      541 tgcgcgcggg acaacccctg acctgcttcg acgacttcgg cgcgcaaccg cagaagctcc
      601 tcctcaccag gcggcgcgga cgattcgaag gccgcgagcg cccgctcggc gcgctcgacc
      661 ggttcgagca accccgccag aaaggggtac tctggatcgg cagacatgcg ctgtatgtac
      721 gtgctggttc gccgcgtcga aacctcggcg cgaaaggcgc gctgcgcggc tccggggcgc
      781 tcgattcggt cgaagcggag gtgagggaag agcgcctcga tgagcggcga attcgcgccg
      841 aggcgcttct cgagttcgcc gaacagctca cgcacggagc cgacccaagc ttgctcgagc
      901 cccgtcagtc gttcggcccg cagcgctttg agttcaggca agtccaggct cgtttcgatg
      961 gctgcgcgca cggctgggat gctcgccgag agtcgccgcg ccgcggccgc gagccaaccg
     1021 cgctcttctc cgccgagcgg cgagtcggtg agcctcgccg ccacgctttc gagccgattc
     1081 acgagacgct cggatgcctc gaggcgctcg aggatcgcgt tcacttccga cttcatcggc
     1141 gcgcaaccta gccgattccg caaggcttgc cagggctctc gggtaagcgc cccagcgcac
     1201 catgtttcag tcgacgccga gctccgtcaa gaagccatcg gctctgacca acgtcgcgtc
     1261 cgcgtcaccg gagtgagtgc ccggcccgtc cagcatcccc gcggagaacc agcgcccatc
     1321 ggcgtctcgc gcgagcgagc gcacctcgtt gtgtcgttgt cggttcggca ccaagagcgc
     1381 gcgtttcgcc ccgccttcgg cgaggacgta agcgaagccc tgactctctt ccgacacgct
     1441 tgcgccatgc ggattctggc tgtatccgct ggcacccacg atcaaaagct cggcatccga
     1501 gagcggcgcc acgtcgaagg cgatgtcact gcgatcgacg tcgagctcgg tgacgcgcac
     1561 ggcgccggtc ttgccatcta cctcagcggc gagcgcgtcg aagccggtgc ccgcgtcgtt
     1621 ccagtattcc ttgcggcccg ccacgtacgc ggcgctcttt cctgcgcgca agccgtagag
     1681 ctcatcgagc tccggcgtgc caacgacgct ggtgccgagt cgcgttccat cgagagcgag
     1741 gcgggttacg taaatgtcga gcgagtcggg gtcgccgacg agatcctcgc cgaacacctt
     1801 cttgtgcgct ttcaccaggt tggcgctgcc gtcgtgaggg tgctgcacgg caacgtagat
     1861 ggtggacgcg tcgtcgacgg ctaggtgaac cgcgaactga gtgtcgagct gcccgaaggt
     1921 atcgtggcta ccgccggtca agccgacgcc gtacatcgac actgggggca cgaccaacgc
     1981 tcgcatctcg ggctcgaaat gcgcgtccct gaagtcgtaa cggtatgcga tgaccgaatg
     2041 tcgcccggtg cgagtcgcga tcacgacttg ctccccgagc ggctgcaagc ggcccgtgtc
     2101 gtgggtgttc ggctcgattg gcgtggccgg cgcgtcctcg ggcaagctcg gag
//
LOCUS       DASTKS010001172         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1303671, whole genome shotgun sequence.
ACCESSION   DASTKS010001172 DASTKS010000000
VERSION     DASTKS010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303671"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /gene="murD"
                     /locus_tag="VFQ35_24940"
     CDS             complement(<1..1252)
                     /gene="murD"
                     /locus_tag="VFQ35_24940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9933983.1"
                     /translation="MKVMQGARVIVVGLGRSGIAAAKLCRDRGAHVIGTDSAPLEKVS
                     PEAKALGIELIAGGHAGVPFTTSDLVVVSPGVPPLPELEAAEAAGVEVIGELELACRS
                     ITAPILAVGGTNGKSTTTTLLADLLRASGKKIFAGGNLGTPAAEAVGVDYDAVVLEVS
                     SFQLERAPAFHPRVSLLLNITEDHLDRYPSFLAYAEAKGNAFVNQTPDDFAIVPSDDP
                     ECERQARRGRGRLVTFGARADYVVDAHGVVESESGERFDLEATRLFGRHNRENAAAAL
                     AAARAMEVPALAARRALVEFTPLAHRMALAGTVSGVRFYDDSKGTNVGASVTALRGLS
                     EARGVLIAGGRDKHGSYEPLVEALVERGRAAVLIGEAAERIENAIAGRIRVERAGSMD
                     DAVKTAFRLAERGDAVLLSPACSSF"
     gene            complement(1259..>2173)
                     /gene="mraY"
                     /locus_tag="VFQ35_24945"
     CDS             complement(1259..>2173)
                     /gene="mraY"
                     /locus_tag="VFQ35_24945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9933984.1"
                     /translation="GTPTMGGALILVSLLVPTVLWADVKNVFVVATAAVTAGYGAIGF
                     LDDRLKIEGSNTRGLPGRYKLLFQFLVGGLVMAFLFLHDQGLPKAWLEIRGRLAVPFL
                     AFDKHPIELPIVLYAIFAMFVVVFMSNAVNLTDGLDGLAIGPVMINAGTYLAWAYVAG
                     SVLFGRPLAGYLDVAGIPEMAELSVYAAAVIGSGIGFLWYNTYPAQVFMGDVGSLSLG
                     GGLGMLAVLTKTEMLSVILGGVFMVEAVSVIVQVTSFRLFKKRVFLMAPIHHHFEKKG
                     WPEPRIIVRFWIISVLLALVALSSLKLR"
BASE COUNT          390 a          757 c          695 g          331 t
ORIGIN      
        1 cgaagctcga gcacgccggg ctgagcagta ccgcgtcgcc gcgctcggcc aagcgaaacg
       61 cggtcttgac ggcgtcatcc atactccccg cccgctcgac gcgaatgcgg ccggcgatgg
      121 cgttctcgat gcgctcggcc gcttctccga tgagcaccgc ggcgcgcccg cgttccacga
      181 gcgcttcgac gagaggctcg tacgagccgt gcttgtcgcg cccgcccgcg atcaagacgc
      241 cgcgcgcctc gctgaggcca cgcagcgccg tcaccgacgc gcccacgttg gtgcccttcg
      301 agtcgtcgta aaaccggact cccgaaacgg ttccggcgag ggccatgcga tgcgccagcg
      361 gcgtgaactc gacgagagcc cggcgcgcgg ccagggccgg tacttccatc gcccgcgccg
      421 ccgcgagcgc ggccgctgcg ttctctcggt tgtgccgccc gaacaggcgc gtcgcttcga
      481 ggtcgaagcg ttctccgctt tcgctctcga caacaccgtg cgcgtccacc acgtagtccg
      541 cgcgcgcgcc aaaagtgacg agacgccccc tgccccgtcg cgcctggcgc tcgcactcgg
      601 ggtcgtcgct cggcacgatc gcgaaatcgt ccggcgtttg gttcacgaac gcgttgccct
      661 tcgcttcggc gtacgcgaga aagctcggat agcggtcgag gtggtcctcg gtgatgttga
      721 ggagcaagct cacccgaggg tgaaacgccg gcgcgcgctc gagctggaaa ctcgagactt
      781 cgagcaccac cgcgtcgtag tcgactccga ccgcttcagc ggcgggagtg cccaagttac
      841 cgcccgcgaa gatcttcttt ccgctcgcgc gcaacagatc ggcgaggagg gtcgtcgtcg
      901 tgctctttcc gttggtgccg ccgaccgcga ggattggagc cgtgatcgag cgacacgcga
      961 gctcgagctc accgatcact tccactcccg ccgcctccgc ggcttcgagc tctggcaggg
     1021 gcggcacccc gggcgacacc accacgagat cgctcgtcgt gaacggcacg cccgcgtgcc
     1081 cacccgcgat gagctcgatg cccaacgcct tcgcctcggg gctcaccttc tcgagcgggg
     1141 cgctgtcggt cccaatcacg tgcgcgccgc ggtcgcggca gagctttgcg gccgcgatgc
     1201 cgctcctacc gagacctacg acgatgacgc gcgcgccttg catcactttc atagttcctt
     1261 atcgaagctt gagcgaggag agcgccacga gcgcgagcag caccgagatg atccagaagc
     1321 ggacgatgat gcgcggctcg ggccagccct tcttctcgaa atgatggtga atcggcgcca
     1381 tcaagaagac gcgcttcttg aacaggcgaa acgaggtgac ctggacgatg acgctcaccg
     1441 cctcgaccat gaagacgccg cccaggatca cgctcagcat ttcggtcttg gtgagcacgg
     1501 ccagcatgcc gagcccgccg ccgagcgaga gcgagcccac gtctcccatg aacacctggg
     1561 ctgggtacgt gttgtaccaa aggaagccga ttccagagcc aatcacggcc gcggcgtaga
     1621 cgctgagctc cgccatttcg gggatgcccg cgacgtcgag gtaacccgcg agcggtcgac
     1681 cgaagagcac ggagccggcc acgtaagccc acgcgaggta cgtacccgcg ttgatcatca
     1741 ccgggccgat ggcgagcccg tcgaggccgt cggtgaggtt caccgcgttg ctcatgaaga
     1801 cgaccacgaa catcgcgaag atcgcgtaga gcacgatcgg cagctcgatg gggtgcttgt
     1861 cgaacgcgag gaacggcacc gcaaggcgcc cgcgaatctc gagccaggct ttcggcaagc
     1921 cctggtcgtg caagaagaga aacgccatca cgaggccgcc gaccaagaac tgaaagagca
     1981 gcttgtaacg gccgggaagc ccgcgcgtat tgctgccctc gatcttcaga cgatcgtcga
     2041 ggaagccgat cgcgccgtag ccggccgtca ccgcggccgt cgcgaccacg aacacgtttt
     2101 taacgtccgc ccagagcacg gtcgggacga gcagcgacac caagatgagc gcaccgccca
     2161 tcgtcggcgt gcc
//
LOCUS       DASTKS010001173         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1303824, whole genome shotgun sequence.
ACCESSION   DASTKS010001173 DASTKS010000000
VERSION     DASTKS010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303824"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFQ35_24950"
     CDS             complement(<1..540)
                     /locus_tag="VFQ35_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933985.1"
                     /translation="MSEQIDVEVQTPRPPDSSNEASVGRFSPRLELTTERMWITDENG
                     EEREDDVPVLLLTFDYGGTEIRASDRRERFFVAQHGGLRSVSRDRAGEARAQCLLESF
                     GAVELSCAEGYLAPDDSHADYLVHTDGNVHAFCSFTAYAVPQLRALGWDVQIRDGYPY
                     RVVSDQAAWYAGLEPGDSDE"
     gene            complement(1255..>2349)
                     /locus_tag="VFQ35_24955"
     CDS             complement(1255..>2349)
                     /locus_tag="VFQ35_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9933986.1"
                     /translation="YGTVETAVQAMREGAYDFVEKPLKRVTIVKSVRKAAERHLLVAE
                     NRSLRQEIRLLKHRDIVGQSPALRRVLDIATQAAPSTATVLILGESGTGKELIARFIH
                     SKSARANGPFIAVNCAAIPESILEAELFGHERGAFTGATTRREGRFARARGGTLFLDE
                     IGELSPAVQVKILRVLQEGEYEPIGGRTEKSDARIVAATNRDLLAEVEAGRFREDLYY
                     RLNVIALTAPPLRARREDIPLLADHFLGVYCKKNGRARLSVAPEVLTKLSDYSWPGNV
                     RELENVIERAAVLCPSDVIGLGDLPEAIAEATAPAPSSLSFPLGTPLAEVEQRLIRET
                     MKYTGGDKSLAAQLLGISTRTIYRKLGEMP"
BASE COUNT          346 a          755 c          809 g          439 t
ORIGIN      
        1 ctcgtcggaa tctcccggct ctagtcccgc gtaccaggcg gcttggtccg agacgacgcg
       61 ataggggtaa ccgtcgcgga tttgtacgtc ccagcccagc gcgcgaagct gcggcaccgc
      121 ataggcggtg aaggaacaaa aggcgtggac gttgccgtcc gtgtgcacga gatagtcggc
      181 gtgcgagtcg tccggagcga ggtagccctc cgcgcacgat agctcgacgg cgccgaacga
      241 ttcgagcagg cactgggcgc gcgcttcgcc ggcgcgatcg cgggagaccg agcgcaagcc
      301 accgtgttgc gcgacgaaga agcgctctcg ccggtcactg gcgcggatct cggtgccgcc
      361 atagtcgaag gtgagcagca gcaccggcac gtcgtcttcg cgctcctcac cgttttcgtc
      421 ggtgatccac atgcgctcgg tggtgagctc gagccgcggc gaaaagcgcc caacgctggc
      481 ttcgttgctg gaatcaggcg ggcgcggcgt ttggacttcg acgtcgatct gctcgctcat
      541 gcggtctcgc cgggggtcgc ggttccggtc atcgaggcgt gcaacggact gctcgatgtg
      601 ggtgcggcac tccttgggac tgcctgattt ttcggcgcgc tcatgtttcg gagctcttcc
      661 ttcaaacaga aaaaaacctc cctgtccatc gcttctcgca ggggcatcgc tcgacgaaga
      721 cgctttcgtg ggtcgctccg tcgctcctcg atgcgtgcga cgcgcgacgt ccttcgccgt
      781 tcacgaccgc cgttcacgac ggcgtggcgc gggcgcggct tcggacccgc gcatcgcttg
      841 gcgtcgatgg caaaaaggcc cgaacgcgct tgctctgcgc gcacttttcg gagcctcgcc
      901 cccacgagcg aaccaaatct cgggcctccg atcgaacgag ccgcgcacca cttgcgctcg
      961 agaggtggaa gtcgcgccgt cgttcacgaa cttcaagttt tggatcaacg attcccccca
     1021 aagcggccgg cgtcgctttc gttaacgcag cgtcgtcgaa ccatgcggcg ttcgtcccgt
     1081 gcgggctccc atgtggcgtc gtccctgggg acccttcgcg ccgcattcgg aacccgcttg
     1141 gctgccgcat tcggaacccg cttgcctgag gcgtatgtgc tcgtgcgcgg tcgcctcgcg
     1201 cggcgggacg ggacgttcga ggcgcgctcg gtgcgggctt cgggctgctc tcggctacgg
     1261 catttcgccg agttttcgat agatcgttcg cgtcgagatg ccgaggagct gcgcggcgag
     1321 cgatttgtcg ccgcccgtgt acttcatggt ctcgcggatc aaccgctgct cgacctcggc
     1381 gagcggcgtg cccagcggga agctgaggct gctcggggcg ggggcagttg cttcggcgat
     1441 cgcctcgggc aaatcgccga gaccaatcac gtcggagggg cagagcaccg cggcgcgctc
     1501 gatgacgttc tcgagctcgc ggacgttgcc gggccaggag tagtcgctca gcttggtgag
     1561 cacctcggga gcgacgctga ggcgcgcgcg gccgttcttc ttgcaataga cgccgaggaa
     1621 gtgatcggcg aggaggggga tgtcttcccg gcgagctcgc agcggcggcg cggtgagggc
     1681 gatgacgttc agtcggtagt agaggtcctc gcggaaccga ccggcttcga cctcggccaa
     1741 gaggtcgcgg ttcgtggccg cgacgatgcg cgcgtccgac ttctcggtgc gcccgccgat
     1801 cggctcgtat tcgccctctt gcaggacccg caagatcttc acttgcacgg cggggctgag
     1861 ctcgccgatt tcgtcgagaa aaagggtgcc gccgcgcgct cgcgcaaagc gcccttcgcg
     1921 gcgcgtggtc gcccccgtga acgcgccgcg ctcgtgaccg aagagctccg cctccaaaat
     1981 gctttcgggg atcgcggcgc agttgacggc gatgaagggt ccgttcgcgc gggcgctctt
     2041 gctgtgaatg aaacgggcga tgagctcttt gccggtgccg ctctcgccga ggatgagcac
     2101 ggtcgccgtc gacggcgcgg cctgagtcgc gatgtcgagc acgcggcgca gcgctgggct
     2161 ttgcccgacg atgtcgcggt gcttcaagag gcgaatctct tggcgtagcg atcggttctc
     2221 ggcgaccaaa aggtgtcgct cggcggcttt gcggacgctc ttcacgatgg tgacgcgctt
     2281 gagcggcttc tcgacgaagt cgtaggcgcc ctctcgcatc gcctgcaccg ctgtctccac
     2341 ggtgccgta
//
LOCUS       DASTKS010001174         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_186373, whole genome shotgun sequence.
ACCESSION   DASTKS010001174 DASTKS010000000
VERSION     DASTKS010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186373"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFQ35_24960"
     CDS             complement(<1..191)
                     /locus_tag="VFQ35_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933987.1"
                     /translation="MSKSRIKSALTLLFATAVTLSLSVGCSKQGEGERCDHTNAGDTD
                     CEDGLVCTNVAPNQDRCCPP"
     gene            313..669
                     /locus_tag="VFQ35_24965"
     CDS             313..669
                     /locus_tag="VFQ35_24965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET9933988.1"
                     /translation="MAIKVYHYDKCSTCRKALAFLDRAGIEYDAIDIVTRPPSKKELK
                     EALTRTQLPLKKLFNTSGQSYREGRFGERLPELSEAEAIAALNADGKLVKRPLVLGPD
                     FALVGFDEAQYRERFG"
     gene            complement(686..1366)
                     /gene="pgsA"
                     /locus_tag="VFQ35_24970"
     CDS             complement(686..1366)
                     /gene="pgsA"
                     /locus_tag="VFQ35_24970"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9933989.1"
                     /translation="MTTSADSSAGRPKLGAEERQKRRRSLKEDALNLPNLLTIGRVVI
                     IPVVLALMDRGTPKDCAWAAIVYAAAAITDLLDGYLARRMNVVSVLGKFLDPLADKLL
                     VMASLVYMVPMGRIPEWAVVLLLAREISITALRSIASSEGVVIAAGEEGKAKTALQMV
                     GILCLILGYPYHLELGPLDLGVVDLIYVGRALVYVSLVFSILSAVSYLRLFSAAVEAK
                     DAKIERRG"
     gene            complement(1363..>2263)
                     /locus_tag="VFQ35_24975"
     CDS             complement(1363..>2263)
                     /locus_tag="VFQ35_24975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933990.1"
                     /translation="AADAVEELDASADGSAFERAVSDPEVSPLRSGRWQALEPGEDPL
                     PPISRSRRPPLSAHIAPGPPSIQMPSAAPISVSEAPTSAPRLPALPITPDALVNARRL
                     ETGGRTHKTPEGLVHVNVSESFAVRTERIRALRPEFTGLSGKPLKRRMRGREIDEEFG
                     GAGGGFCLIEGKGSLLIEPSSERELTLLGLGGEFVYLREARLVGFDGSLRHENGRLPA
                     ADPGPVPMVQLAGNGVLVFEARRSLRALPVSAERGVTVRAASVLGWTGRLLGHALAAD
                     QALPLGAGFVVFSGDGAVLVDDP"
BASE COUNT          395 a          730 c          777 g          361 t
ORIGIN      
        1 ggcgggcagc aacgatcttg gttaggcgcc acgttggtgc acacgagccc gtcttcgcag
       61 tcggtgtcgc ccgcgttcgt gtgatcgcag cgttcgcctt cgccctgttt ggagcagcca
      121 acactcagag acagcgtcac ggcggttgcg aacagcagcg tcagcgcgct tttgattctc
      181 gacttcgaca tgctgggtgg cttctagcag cgcccgaggc ggattgtcca gccttcgggc
      241 cgacggtccg atgcttgctc tctccgggag cctggcccac gatcgcgccg aaccgagtag
      301 ctcgaggctt taatggcgat caaggtctac cactacgaca agtgcagcac gtgccggaag
      361 gcgctggcgt ttttggatcg ggcgggcatc gaatacgacg caatcgacat cgtgacccgg
      421 ccaccgtcca aaaaggagct caaggaggcg ctcacgcgca cgcagcttcc gctgaagaag
      481 ctcttcaata cctccggcca gagctaccgg gagggacggt tcggggagcg cctacccgag
      541 ctcagcgaag cggaagcgat tgccgcgctc aacgccgacg gcaaattggt gaagcggccc
      601 ctcgtgctcg gccccgattt cgcgctagtc ggcttcgacg aggctcagta ccgcgagcgc
      661 ttcgggtgag cgcgacgacc atcggtcagc cgcggcgttc gatcttcgcg tccttggctt
      721 cgaccgcggc ggagaacagc cgaaggtaag agacggcgct caagatcgag aagacgagcg
      781 agacgtaaac cagggcgcgc cccacgtaga tgaggtcgac cacgccgagg tcgaggggcc
      841 cgagctcgag gtggtatggg tagccgagga tcaggcacag gatgccgacc atctgcaggg
      901 ctgtttttgc tttgccttct tcgcccgccg cgatcacgac cccttcactg ctcgcgatgc
      961 tgcgcagggc agtgatggag atctcgcggg cgagcagcag taccaccgcc cactcgggga
     1021 tgcggcccat cggcaccatg tagacgagcg atgccatgac gagcagcttg tcggcgagcg
     1081 gatcgaggaa tttaccgagc acgctgacga cgttcatgcg ccgggccagg taaccgtcga
     1141 gcagatcggt gatggccgcg gctgcgtaaa cgatggcggc ccaggcgcaa tccttcggcg
     1201 tgcctcggtc catgagggcg agcacgaccg ggatgatgac gacgcgcccg atggtcagca
     1261 gattcggaag attgagcgcg tcttctttga gcgagcgacg gcgcttttgg cgctcttcag
     1321 cgccgagctt gggccggccc gcgctcgaat ccgccgaggt cgtcatgggt cgtccactaa
     1381 cacggcgccg tcaccgctga acaccacgaa cccggcgccg agcggaagcg cctgatctgc
     1441 ggccaaagcg tgaccgagga ggcggccggt ccagccgaga acggacgcgg cccgtaccgt
     1501 aacgccacgc tctgcactga ccggcagcgc gcgaaggcta cggcgcgctt cgaagaccaa
     1561 gaccccattg ccagcgagtt gcaccattgg cacagggccc ggatcggccg ccggtagccg
     1621 gccattctcg tggcgcagcg agccgtcgaa gccgacgagc cgcgcctcgc gcaggtagac
     1681 gaactcgccc ccgaggccga gcagggtgag ctcgcgttcg ctgctcggct cgatgagcag
     1741 cgagcctttg ccctcgatta ggcaaaagcc gccccccgcg cccccgaact cttcgtcgat
     1801 ttcgcggcct cgcatgcggc gtttgagcgg cttacccgag agcccagtga attcgggccg
     1861 caacgcgcga atgcgctccg tacggaccgc gaagctttcg ctcacgttga cgtgaacgag
     1921 gccctcgggg gttttgtggg tgcgcccacc ggtttcgagg cgccgcgcgt tcacgagcgc
     1981 gtccggggtg atgggcaaag cggggagccg cggcgcgctg gtcggcgcct ccgaaaccga
     2041 gattggcgcc gcgctcggca tttggatgga gggagggcca ggcgcgatgt gcgcgctcag
     2101 gggagggcgg cgcgagcggc tgatcggcgg cagcgggtct tctccgggct cgagtgcctg
     2161 ccagcgcccg ctacgcagcg gcgaaacctc gggatccgaa accgcgcgct cgaaggcgct
     2221 gccatcggcg ctcgcgtcga gctcttcgac ggcatcggcg gcg
//
LOCUS       DASTKS010001175         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1304070, whole genome shotgun sequence.
ACCESSION   DASTKS010001175 DASTKS010000000
VERSION     DASTKS010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304070"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFQ35_24980"
     CDS             <1..852
                     /locus_tag="VFQ35_24980"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HET9933991.1"
                     /translation="LMSKEGGDAKRFVLLGTDYVYPRTTNKILRYFLQSKGVAEADIM
                     EKYTPFGHTDYQTIIAEIKKFAAGKRTAVVSTINGDSNVPFYKELGNQGVKAEDIPVV
                     AFSVGEEELRGIDTTPLVGQLAAWNYFMSIDTPENKEFIAKWMAYVKKNNLPGGDKRV
                     TNDPMEATYIGIKMWAQAVTQAGTTDVDAVRQAVAGQTVKAPSGFDIKMDEKNHHLHK
                     PVFIAEVKADGQFQVVEKTEGPIRAQAWSPFIPESSKKVADWTFPWVCGNCTEPKFKT
                     ESAVAEK"
     gene            977..2593
                     /gene="urtB"
                     /locus_tag="VFQ35_24985"
     CDS             977..2593
                     /gene="urtB"
                     /locus_tag="VFQ35_24985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET9933992.1"
                     /translation="MKQLVRISWLILSLILLVAPAARAEALPELAGLSAEDPALVAKT
                     VDAIAARHDAVALKVLSALLEGNLRVDAKGLPYAVGKGDAYEALVPGAAPKPSPPLAT
                     PMVNNQLRRQLEPTIAALKLSAPDRETRLAAAEDLAKSRNDDLAPLVRKALASEKDAK
                     IRERLQIALAQVDLASTDKAKRLSAIAAIKAAADANFKPDLERLIARDEKNQFREPDE
                     DIRRAASAALGAIETRATIYGFVGNLFYGLSLGSVLLLAALGLAITFGLMRVINMAHG
                     ELLMIGAYATYSVQKLFLQYLPDHSDFYIIAAVPAAFIASGAVGLLIERTVLRFLYGR
                     PLETLLATWGISLMLIQTVRLLFGAQNVTVSNPSWLAGGVEVLPGFVLTYSRLAVILF
                     SGVVVAFVWFMLQRTPLGLHVRAVTQNRAMAAGMGISTRRVDSWTFAIGSGVAGLGGV
                     ALSQLGNVGPELGQGYIVDSFMVVVLGGVGKLAGTLAGAFGLGIVNKLLEPVAGAVLG
                     KIFILAFIILFIQRRPQGIFALKGRAAEVA"
     gene            2590..>2914
                     /locus_tag="VFQ35_24990"
     CDS             2590..>2914
                     /locus_tag="VFQ35_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933993.1"
                     /translation="MSAPATKQEATELAPPSAEQVLESKEAPSAAAGEGAAGEVSVDR
                     AEGSASGADALAAAGGASPPAVADKRLEPILRKRGWIAFGVVTVLAVALVPILNSLPT
                     ASALHL"
BASE COUNT          523 a          948 c          944 g          499 t
ORIGIN      
        1 ttgatgagca aggaaggcgg cgacgcgaag cgcttcgtgc tgctcggaac cgactacgtc
       61 tacccgcgca cgacgaacaa gatcttgcgt tacttcttgc agagcaaagg tgtcgccgag
      121 gccgacatca tggagaagta cacgccgttc ggtcacaccg actatcaaac catcatcgcc
      181 gagatcaaaa agttcgcggc gggcaagcgc accgcggtcg tctcgacgat caacggcgac
      241 tcgaacgttc cgttctacaa agagctcggc aatcagggcg tgaaggccga agacattccg
      301 gtcgtcgcgt tctcggtcgg tgaggaagag cttcgcggta tcgacacgac tccgctcgtc
      361 ggtcagctcg cggcgtggaa ctacttcatg tcgatcgaca cgccggagaa caaagagttc
      421 atcgccaagt ggatggcgta cgtgaagaag aacaacctgc ccggcggcga caagcgcgtg
      481 acgaacgatc cgatggaagc gacgtacatc ggcatcaaga tgtgggcaca agcggtgacg
      541 caagccggaa cgacggatgt cgacgcggtg cgtcaggcag tggcaggcca gacggtgaag
      601 gcgccgtcgg gcttcgacat caagatggac gagaagaacc atcacctgca caagccggtg
      661 ttcatcgccg aggtgaaggc cgacggtcag ttccaggtcg tggagaagac cgagggtccg
      721 atccgcgctc aggcctggag ccccttcatc cccgagagct cgaagaaggt cgccgactgg
      781 accttcccct gggtttgcgg gaactgcacc gagcccaagt tcaagaccga gagcgccgtc
      841 gccgaaaaat gacgtgcgcg tgagctgagc gagctggggc gccgtttccg cggcgcttcg
      901 gctcgatcct cgaaagacca ccgttacgag accgtcgccg ctcgcatcga gagcggcccc
      961 agttggcgag gcttggatga aacagctcgt gcgcatcagt tggctaatct tgagtctgat
     1021 tttgttggtg gcccccgctg cgcgagccga ggcgcttccc gagctcgctg ggctctccgc
     1081 cgaggatccc gcgctcgtcg ccaagacggt ggacgccatc gcagcgcgcc acgacgcggt
     1141 tgcgctgaag gtcctctcgg cgctcctcga gggcaacctg cgcgtcgacg caaagggtct
     1201 gccctacgcc gtggggaagg gagacgctta tgaggcgctc gtccccggcg ccgcgccgaa
     1261 gccctctccg cccctcgcga cgccgatggt gaacaaccag ctgcgacgcc agctagaacc
     1321 gacgatcgcc gcgttgaagc tcagcgcgcc ggatcgcgag acgcgactcg ccgccgccga
     1381 agatctcgcc aagagccgca acgacgatct cgcgcccctc gttcgcaagg cgctcgcctc
     1441 cgaaaaggac gcgaagatcc gcgaacggct ccagatcgcg ctcgcgcagg tcgatctcgc
     1501 gtcgaccgac aaagcgaagc ggctttctgc catcgccgcg atcaaggcgg ccgccgacgc
     1561 caacttcaag cccgatctcg agcgactcat cgcgcgcgac gagaagaacc agtttcgtga
     1621 gcccgacgaa gacatccgcc gcgccgcgag cgccgcgctc ggtgccatcg aaacgcgcgc
     1681 gacgatttac ggcttcgtag gaaacctgtt ttacggcttg agcctgggca gcgtgctctt
     1741 gctagcggcg ctggggctcg ccatcacctt cggcctcatg cgcgtcatca acatggctca
     1801 cggtgagctc ctgatgatcg gcgcctacgc tacgtactcg gtgcagaagc tcttccttca
     1861 gtacctgcca gaccattcgg atttctacat catcgcggcg gtgccggcgg ccttcatcgc
     1921 gtcgggggcg gtagggctcc tcatcgagcg caccgtgctc cggttccttt acgggcgtcc
     1981 tctcgagacg ctgctcgcca cgtggggcat ctcactgatg ctcatccaga cggtccgact
     2041 gctcttcggg gcgcagaacg tcacggtctc gaacccgagt tggctggcgg gtggcgtcga
     2101 ggtgctgccc ggcttcgtgc tcacctacag ccgcctcgcg gtaatcctgt tcagcggagt
     2161 cgtcgtcgcg ttcgtgtggt tcatgcttca gcgcacgccg ctcggcttgc acgtgcgcgc
     2221 ggtgacccaa aaccgagcga tggccgccgg aatgggcatc tccacgcgcc gcgtcgactc
     2281 gtggactttc gccatcggtt cgggcgtcgc ggggctcggt ggcgtcgctc tgtcgcagct
     2341 cggcaacgtc ggtccggagc tcgggcaagg ctacatcgtc gactccttca tggtggtcgt
     2401 gctcggcggc gtcggaaagc tcgccggcac gctggcgggc gcctttggtc tcggcatcgt
     2461 caacaagttg ctcgagcccg tggcaggcgc ggtgctcgga aagatcttca ttttggcatt
     2521 catcatcttg ttcattcagc gccgtcccca gggcatcttc gccctcaagg gccgcgccgc
     2581 ggaggtcgca tgagcgcccc agcgaccaag caagaggcga cggagcttgc gccgccttca
     2641 gcggagcagg tgctcgaatc gaaggaggca ccgagcgccg cagcgggtga gggcgcagcc
     2701 ggcgaggtga gcgttgaccg cgccgaaggc agcgcgagcg gcgcggacgc cctggcggct
     2761 gcgggcggcg catcgccacc cgcagtggcc gacaagcgtc tcgagccaat cctccgcaaa
     2821 cgagggtgga tcgcgttcgg tgtcgttacg gtgctcgcgg ttgcgctcgt ccccatcttg
     2881 aactcgctgc caacggcctc cgcgctgcat ttgc
//
LOCUS       DASTKS010001176         5423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1528281, whole genome shotgun sequence.
ACCESSION   DASTKS010001176 DASTKS010000000
VERSION     DASTKS010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528281"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..2342)
                     /locus_tag="VFQ35_24995"
     CDS             complement(183..2342)
                     /locus_tag="VFQ35_24995"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HET9933994.1"
                     /translation="MRFGLIHRVMTDALAALGVLALVSSGELDRWLSYGCVIGLVLAL
                     AVPDTIQDRPLMRRFSVFAPLGLFGLQVARLASGAELLPVAVEFAAALQVIRLATRRG
                     AAHDQQVIVLALIHLIAGTVLGGGLSYGLCFLGFLVVAPGALVLSHLRREVEGNYRQG
                     ARDRTGLPVDVPRILRSRRVIGRPFLAFTCLLSLPIFVFTALLFVLFPRVGLSLLLLN
                     HSRPERMVGFSDKVDLGGVGKLRTDPTIVLRVEVPNLPPEPPPRLALYLKGTAFDRYD
                     GKSWSRSQPPSRTPAEQRGHSVRLLRFPERDDRRLRIDLEPIDPQVIFLPIDAVAMTV
                     ITPMNAAPALVPEIFSAPEGGLTYRSPDERGLRYEVSLAGSREDFTVRPLGAEAARYL
                     ALPSNLPVRIHELAREWTKGVSDASEQARLIEARLRQDYRYDLDSPSGAAKNPLDHFL
                     FVSKRGHCEFYSTAMAVLLRSLGVPTRNATGFIGGSYNRFARNYAVRQGDAHSWVEVY
                     LPNRGWTRFDPTPPASSAPRSEIHGLFASLRDIVEALGQRWNHHVVNYDLKQQIGLLR
                     SARKQYRDFVSHTGMLGRAIGSPRRAAITLIVLAGAVLALRFWMRRRRGPVSPRPTTT
                     QQEVAARRIVELYRSLELALAARGVPRPSGTPPLRHAETLVGIGHPIAPEVVLLTQRY
                     QSVRFGGEPLDDAARRDFAERVKAIKLGRDAALPAQL"
     gene            complement(2339..3472)
                     /locus_tag="VFQ35_25000"
     CDS             complement(2339..3472)
                     /locus_tag="VFQ35_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9933995.1"
                     /translation="MKGSALPTPDSARPGGQDAAEGEDPSSSRPVLRRGAPPPPAVDP
                     AKSAFERFVRRWRPPRKLKLTREGKYFIGVTFGVGFAAINTGNNLLYLLLGMLLSLIV
                     VSGVMSELSLRDLGVVRRLPPRAQVGRAHLVEIEVFNHKHRVPSYAIEIEDLRAGQPA
                     DKRCFFLKISPRSTQVAAYRRTPARRGRDKHIGFRVATRFPFGLFEKSREISAQGDLI
                     IYPAVDPVTLPSDGRGERLGGATALGRGSGDEVLGVRPMREGDDPRDIYWRKSTLPNH
                     FVLRERGRETRRDVSFLLESTYKPPTLPNEEWSTRFERRIRDIASRAVAHLKRGDTVL
                     IRTTAGERARATAAGGADPLLRFLALLEARPEPESGQTKEAAE"
     gene            complement(3480..4445)
                     /locus_tag="VFQ35_25005"
     CDS             complement(3480..4445)
                     /locus_tag="VFQ35_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9933996.1"
                     /translation="MTASAHVLEPQRIERLVALRQAVEGALEGKPEVVELALIALLAR
                     GHVLIEDVPGVGKTTLARALARAVGGELRRVQFTSDLLPSDVLGVSVFDQRRAEFTLR
                     HGPIFTNLLLADEINRASPRTQSALLEAMSEGRVSLDGKTVDLPEPFLVIATQNPQDF
                     AGTFPLPESQLDRFMMRLRIGYPPPHVETRLMLEPDADRIDAVPTVLDPQSLVTLQRE
                     VDRVTIDGALGAYLQALLTATRSSPTLSLGASTRAGMNLSRAARARALLHARHYCIAD
                     DIHVLAVPVIAHRVRLASHAEGYSPSREEAETAVREVVARVPVPL"
     gene            4787..5389
                     /locus_tag="VFQ35_25010"
     CDS             4787..5389
                     /locus_tag="VFQ35_25010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9933997.1"
                     /translation="MFGATAALRSVDVSFEAGTISLLEGPNGAGKSTLLSVIGTVLRP
                     TSGDVEYSPVGSEVELVRPHLGWLAHDSHCYRELSVWENLRFAARVHGLPVEQALEDV
                     AGRVEIRHLRDRRVGLLSRGQRQRVALARALIHRPSVLLLDEPWTGLDAASAAHLEEV
                     IREEGAAGTLIVVVSHDDSVGKRLNARRVRLEGGRIKSSE"
BASE COUNT          937 a         1726 c         1885 g          875 t
ORIGIN      
        1 cggcctcgtc gctccgctcc tcgaaaggct tcagccttcc tccggtgctc actcctcggg
       61 cgcgcttctc tctcgcgttt tcttggctct cgcgacgggc aggaacttgg gacaccccct
      121 aacgccgtcc gttcgcccgc ctcacgttcg cggcgcgacc aggggactcg aagtcgctgg
      181 gttcagagtt gagccggcaa agcggcgtcg cggcccaatt tgatggcttt tacgcgctcg
      241 gcgaagtcac ggcgagcagc gtcgtcgagc ggttcgccgc cgaagcgcac gctttggtag
      301 cgctgcgtca gcaggaccac ctcgggcgcg attgggtgac cgatgccgac gagtgtctcg
      361 gcgtgcctca acggcggcgt gccgctgggc cgtggaacgc cgcgcgccgc gagcgcgagc
      421 tcgagtgagc gatagagctc gacgatgcgt cgcgccgcga cttcttgctg agtcgtcgtg
      481 ggcctcggcg agaccggacc gcgtcggcgc cgcatccaaa agcggagcgc gagcactgcc
      541 cccgcgagta cgatgagcgt gatcgcggcg cgtcgcggcg acccaatcgc gcgccccagc
      601 atgccggtgt gcgagacgaa gtcgcgatac tgcttgcgcg ccgaccggag cagaccgatc
      661 tgctgcttca agtcgtagtt gacgacgtgg tggttccagc gctgtccgag ggcctcgacg
      721 atgtcgcgca gcgacgcaaa aagcccgtga atttcgctgc gcggggcgga gctcgcgggc
      781 ggcgtggggt cgaaacgagt ccagccgcga ttcggcaggt acacctctac ccaagagtgg
      841 gcgtcgccct ggcgcaccgc gtagttccgc gcgaagcggt tgtagctgcc gccgatgaag
      901 ccggtcgcgt tccgcgtcgg cacaccgagt gagcgcaaca gcactgccat cgccgtcgaa
      961 tagaactcgc agtggccgcg cttcgagacg aacaagaagt ggtcgagcgg gttcttggcg
     1021 gcaccggagg gagaatcgag gtcgtagcgg tagtcctgac gaagccgtgc ctctatcaag
     1081 cgagcttgtt cgctcgcgtc gctgacaccc ttcgtccatt ccctcgcgag ctcgtggatg
     1141 cgaacgggga ggttggacgg gagcgccagg tagcgcgctg cctcggcgcc gagcgggcgc
     1201 acggtaaagt cctccctgct acccgcgagc gacacctcgt agcggaggcc gcgctcgtcg
     1261 gggctgcggt aggtcaagcc gccctcgggg gcgctgaaga tctcggggac cagcgccggc
     1321 gccgcgttca tcggcgtaat gacggtcatc gcgaccgcgt cgatcgggag gaagatgact
     1381 tgcgggtcga tgggctcgag atcgatccgg agtctgcgat cgtcgcgctc ggggaaacga
     1441 agcagccgga ccgagtggcc gcgctgctcg gcgggagtgc gactcggagg ttggctacgc
     1501 gaccagctct tgccgtcgta tcggtcgaac gcggtgccct tgaggtagag cgccaagcgt
     1561 ggtggcggct ctggcgggag attcgggacc tcgacgcgca gcacgatggt cgggtccgtg
     1621 cgcagcttgc ccacgcctcc gagatccacc ttgtcggaga accccaccat gcgctcgggg
     1681 cgagagtggt tgagcagcag cagcgacagc cccacgcgcg gaaacagcac gaacaagagc
     1741 gcagtaaaaa cgaagatcgg caacgagagc aggcacgtga aggcgagaaa aggcctgccg
     1801 atgacgcgtc gcgagcgcag gatgcgcggc acgtcgaccg gcaaccccgt gcgatcgcgc
     1861 gctccttgcc ggtagtttcc ttcgacctcg cggcgcaaat ggctgagcac cagtgcgccc
     1921 ggcgcgacga ccagaaaacc caaaaaacaa aggccgtacg agagaccccc tccgagcacg
     1981 gtgccggcga tgaggtgaat caacgcgagc acgatgactt gctgatcgtg cgccgccccg
     2041 cgccgagtcg cgagacgaat cacctgcagt gcggccgcga actccaccgc gactggcaag
     2101 agctccgctc cgctcgccaa gcgagcgact tggagcccga acaggccgag cggcgcgaac
     2161 acgctgaagc gccgcatgag cggtcgatct tgaatcgtat cgggaaccgc gagggcgagg
     2221 acgagcccga tgacgcagcc gtagctcagc caccgatcga gctcgccgct gctcaccaac
     2281 gcgagcaccc cgagcgcagc gagcgcgtcg gtcatcacgc ggtgaatcag gccgaacctc
     2341 actccgcggc ctccttggtc tggccagact ccggctctgg acgcgcctcg agcagggcga
     2401 ggaagcgcag cagcggatcg gcgccaccgg ccgcggtcgc acgcgcgcgt tcaccggcgg
     2461 tcgttcggat caacaccgtg tcgccccgct tcaggtgcgc gacggcgcga gaggcgatgt
     2521 cgcgaatgcg tcgctcgaag cgagtgctcc attcctcgtt cggcagagtt ggcggcttgt
     2581 aggtcgattc gagcaagaag ctcacgtcgc gccgcgtctc gcgaccgcgc tcacgcaaga
     2641 cgaagtgatt cggcagcgtg ctcttgcgcc agtagatgtc gcgcgggtcg tcgccttcgc
     2701 gcatcgggcg cacgccgagg acctcgtcac cgctgccgcg tccgagcgcc gtcgcaccgc
     2761 cgagccgctc tccgcggccg tcgctcggaa gcgtcaccgg gtcgaccgcc gggtaaatga
     2821 tgaggtcgcc ctgagcgctg atttcgcggg atttctcgaa gagaccgaag ggaaagcggg
     2881 tcgcgacgcg gaagccgatg tgcttgtcgc gaccgcgccg ggcgggggtt cgtcggtaag
     2941 ctgcgacttg cgtcgagcgg ggactgatct tcaaaaagaa gcagcgcttg tcagcgggct
     3001 gcccagcgcg caggtcctcg atctcgatgg cgtacgaggg gacgcggtgc ttgtggttga
     3061 acacctcgat ctcgacgagg tgagcgcgcc cgacttgtgc gcgcggaggg agtcgacgca
     3121 cgacgccgag gtcgcgcaag ctgagctcgc tcatcacgcc cgaaacgacg atgagcgaca
     3181 gcagcatgcc gagcagcaga taaagcagat tgttgccggt gttgatggca gcgaacccga
     3241 ctccgaacgt gactccgatg aagtactttc cttcgcgggt cagcttgagt ttgcgtggtg
     3301 ggcgccaacg ccgaacgaag cgttcgaagg cggacttcgc aggatcgact gcaggtgggg
     3361 gcggagctcc acgacgcagc accggtcgag acgaagacgg gtcttctccc tcggcggcgt
     3421 cctgcccgcc gggacgagcg ctgtccggag tcggcagggc cgagcccttc attcgttcct
     3481 cagagcggta cgggcacgcg cgcgacgacc tcgcgcacgg cagtttcggc ctcctcgcgc
     3541 gaaggcgaat agccctccgc gtgtgaggcc aagcgaactc ggtgcgcgat tacgggcacg
     3601 gcgagcacgt ggatgtcgtc ggcgatgcag taatggcgag cgtgtagcaa cgcgcgagca
     3661 cgcgcagcac gcgaaaggtt catgccggcg cgtgtcgagg caccgagcga cagggtcggg
     3721 ctcgagcgcg tcgcggtcag caacgcttga aggtaagcgc cgagcgcgcc gtcgatggtc
     3781 acccggtcga cttcgcgttg cagcgtgacg agcgactgcg gatcgagcac cgtcggcacc
     3841 gcgtcgatgc ggtcggcgtc gggctcgagc atgaggcggg tctcgacgtg tggcggcggg
     3901 tagccgatgc gaagccgcat catgaaacgg tcgagctgcg actctggcag ggggaaggtc
     3961 cccgcgaaat cttgcggatt ttgcgtggcg atgacgagga acggctccgg cagatccacc
     4021 gtcttgccgt cgagcgacac gcgaccttcg ctcatcgcct cgagcagcgc cgactgagta
     4081 cgaggactcg cacgattgat ctcgtccgcg agtagcaggt tcgtgaagat gggcccgtga
     4141 cgaagcgtga actccgcccg gcgctgatcg aagacgctca ccccgagcac gtcgctcggc
     4201 agcaagtcgc tggtgaactg aacgcgtcgg agctctccac cgacggcgcg ggccaacgcg
     4261 cgcgccaaag tggtcttgcc cacgccgggg acgtcctcga tgagcacgtg cccgcgagcg
     4321 agcaaggcga tgagcgcgag ctcgacgacc tcgggcttgc cctcgagcgc tccttccacg
     4381 gcttggcgca gtgcgacgag tctctcgatg cgttgcggct cgagaacgtg ggcggaggcc
     4441 gtcatcttcc gagcatcgta gcatccgggg ggaaggaagc ttggtcaagg ataatccgag
     4501 agcgtcgttt cggcactgct cggctttcaa ggtaggtaaa tctaggcggg cgcactccga
     4561 gctcggggcc gggcctgcct tgacaagccc catattcgcg gcggaagttg ccgccgtctc
     4621 gagtcctctc gactccctgg atgtctccgt taggaactac ctatgtcggc cgtttggcgc
     4681 cactcgtcgg cttcgtcggt gggaacgcga gcgctcgact ccttgcttcg ggccggtaaa
     4741 tcgcgtgggc acgattcagc gtatcgatgt tcgacgagtc actcggatgt tcggggcgac
     4801 tgcggccctt cgtagcgtcg acgttagctt cgaggccgga accatctcgt tgctcgaggg
     4861 accgaacggg gccggaaaga gcacgctcct atccgtgatt ggaaccgttc tcaggccgac
     4921 ctccggtgac gtcgaatatt cgcccgtcgg ttcagaagtc gagctcgtgc ggccgcacct
     4981 ggggtggctc gcgcacgact cccattgcta ccgtgagctc agcgtctggg aaaacctgcg
     5041 tttcgccgcg cgggtgcatg gactacccgt cgagcaagcg ctcgaggacg tcgccggccg
     5101 agtcgagatc cggcatttga gggatcgccg cgtggggctt ttgtcgcggg gtcaaaggca
     5161 gcgcgtcgct ctggcgcgag ccctgattca tcgccccagc gtgctcctgc tcgacgagcc
     5221 gtggacgggg ctcgacgctg cgagcgccgc tcacctcgaa gaagtgattc gcgaagaagg
     5281 agccgccggc acgctgatcg tggtcgtgag tcacgacgac agtgttggaa agcgcctgaa
     5341 cgcccgtcga gtgcgcctcg aaggcggccg aatcaagtcc tccgagtgag catcggtcct
     5401 ttccaacgcc tgccgagctc ggc
//
LOCUS       DASTKS010001177         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1079754, whole genome shotgun sequence.
ACCESSION   DASTKS010001177 DASTKS010000000
VERSION     DASTKS010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079754"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..1358
                     /locus_tag="VFQ35_25015"
     CDS             348..1358
                     /locus_tag="VFQ35_25015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9933998.1"
                     /translation="MLSASLLSAYSPSVWAEPTEGDRQVARALAAEGYEALQRKDYAT
                     AEDRFRRADKLVHAPTLIVDLARSLTGLGRLVEAYEYYELVLREGVSPNTPWSWQKAY
                     RDAERELEQIKPRLSWLTINVGGPRKPVVFIDGRRVPSAAVGVPRAVNPGLRMVAARA
                     RGFASKQRQIMVAEGQHETLELNLEPKPEGSPEEPEPVEVEVLDELPPPRLETPGAQR
                     TIGYVALTVGGVGLIVGGVSSVLVLRTRSELARNCPTQVCDPATSPQRSDFQQDLNRY
                     SLFKTAATVSLITGVVGVAAGTYLLFSKGGSPHVESGAKKASLEIEPVLGVDTVGVAG
                     RF"
     gene            complement(1476..2228)
                     /locus_tag="VFQ35_25020"
     CDS             complement(1476..2228)
                     /locus_tag="VFQ35_25020"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET9933999.1"
                     /translation="MVTAVSCLLASATAATADEPSKLQSSAALPAPTATPQAPNASAR
                     DASVTANVDPSDDAVVAPPEALPDCAARLERRGVRFEPAALPLVQKHGFVCGAPQAVK
                     YLRGPEGISFSPRPVTTCQLALGLARFESLAQTLALRYFGVRIKSIRQGGTYNCRPMA
                     RFRLVSEHSYGNAIDLYEFTLENGKRINVLRHFGKPGEQPSGKEGRFLRELAQAAFDE
                     GAFSVVVTRFFDELHRDHIHVDMAHYRTDGSR"
     gene            complement(2304..3206)
                     /locus_tag="VFQ35_25025"
     CDS             complement(2304..3206)
                     /locus_tag="VFQ35_25025"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9934000.1"
                     /translation="MNINNVNLNLLVAFQALFEERSVTRAARRAGITQPAMSNTLAQL
                     RALLDDPLFTRTGKGLLPTARATALADPIGRGLKLLDSALVEQRFDPARSDRLFTVAA
                     SDYVEFVLLPPLLAELARIAPRVRLRIRPWGSHSVPAELARGEFDSMIGFYDDVPARH
                     RHTLLFSDEYVCVVRKGHPRVKHRLTLPRYLELSHVLVSTSNDSPGSVDRALAAQGLR
                     RQVGLRVSHFLSVPMVVARTDYVAALDRRVAQAFSKALGLVLLKPPLALPVGKVGQVW
                     HESVDADPGHRWLREVIASVSSKL"
     gene            3313..>3512
                     /locus_tag="VFQ35_25030"
     CDS             3313..>3512
                     /locus_tag="VFQ35_25030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934001.1"
                     /translation="MTNIQTESNREISENELAVRIASGRAPLIAEILGPSYFAQGHLP
                     GAVNLPLEGLVERATALFPDRSA"
BASE COUNT          609 a         1102 c         1160 g          641 t
ORIGIN      
        1 cgaccgattg cacgagctgc tggtgcacgc tgacgctcgg gaattgctat gcgtacgcct
       61 tctcgccacg cttgctcgag gggcgtctgc tcccgagtcg gccgttcgcc gcgcgaaaga
      121 gcgagccgcg gtcgcgggcg ggacgcgttc gactgccgct cgttcgctga ctcagtttca
      181 tattgggccg ctttcgcggc acgcggcccc agtgtggcgc cttcgcgttg ctcgcgacga
      241 ggttcctcgt tgcttgcgat gccgatccgg gcaggctaaa ccctgtctcc ttgggagatc
      301 atacagagat atggctccgg cgtgttggtc gtcacgtcgc tgtcgcggtg ctgtcggcat
      361 cgctgctctc tgcttactcg ccaagcgttt gggccgagcc gaccgagggt gaccgtcaag
      421 ttgccagggc gcttgccgct gaaggctatg aggcgttgca gcgtaaagac tacgccaccg
      481 ctgaagatcg ctttcgtcgc gcagacaagc tggtccacgc tcccacgctg atcgtcgact
      541 tggcgcggtc gctcaccggg cttggccgac tggtcgaggc ttacgagtat tacgagctcg
      601 tattgcgtga aggtgtgtcg ccgaacacgc cgtggtcttg gcagaaggct tatcgcgacg
      661 ccgagcgcga gttggagcag atcaagcccc gcttgtcgtg gctcacgatc aatgtgggtg
      721 gcccgcgtaa gcccgtcgtg ttcatcgatg gtcgcagagt gccctcggcc gcggtcggtg
      781 tcccgcgcgc cgtcaatcct gggcttcgca tggttgctgc gcgcgcacgc ggcttcgcgt
      841 cgaagcagcg ccagatcatg gttgcggaag gccagcacga gacgctggag ttgaacctcg
      901 agccaaagcc cgagggttca ccagaagagc cggaacccgt cgaagtcgag gtgctggacg
      961 agctaccgcc accgcggctg gaaacgcccg gagcccagag gacgatcgga tacgttgcgc
     1021 tcacggtggg tggagttggc ttgatcgttg gcggtgtctc gtcggtgctc gtcttgcgga
     1081 ctcgctccga gctcgcgcgc aactgtccaa cgcaggtgtg cgatcccgcg acttcacccc
     1141 aaagaagcga cttccagcag gacctcaacc gctacagtct gttcaagacc gcggcgacag
     1201 tgagtctcat caccggcgta gtcggtgtgg ctgccggaac gtatctgctg ttcagcaagg
     1261 gcggttcccc ccacgtcgag agcggcgcga aaaaggcttc gctcgagatt gaaccggtcc
     1321 tcggggtcga tacggttggc gtagcgggtc ggttttgagt cggggcgccc ctcgttcact
     1381 caatgcacgc gcagcgctcg gttcagcggg ctacgggtga agtccaagcg gcctcggccg
     1441 cgactgagcg gaatgcagga aatcgggcgc tggactcagc gcgagccgtc cgtgcggtag
     1501 tgggccatgt cgacgtggat gtggtcccgg tgcagctcat cgaaaaagcg cgtcacgacc
     1561 accgagaaag cgccttcgtc gaaggcggcc tgcgctagct cgcgcaggaa gcgcccctcc
     1621 ttacccgagg gttgttcgcc gggcttgccg aaatggcgca gcacgttgat tctcttcccg
     1681 ttctctagcg tgaactcgta aaggtcgatg gcgttaccgt agctgtgctc gctcacgagc
     1741 cgaaagcgcg ccattggtcg gcagttgtac gtgccgcctt ggcggatgct cttgatgcgc
     1801 actccaaagt atcgaagcgc tagggtttgc gccagtgact cgaagcgcgc caaaccgagc
     1861 gcgagttggc aggtggtcac ggggcgaggc gaaaacgaaa tgccttccgg gcctcgcagg
     1921 tacttcaccg cctgcggtgc cccgcagacg aagccgtgct tttggaccaa cggtagcgcc
     1981 gcaggttcga atcgcactcc gcgtcgttcg agccgcgccg cgcagtccgg cagagcctcg
     2041 ggcggtgcca cgacggcgtc atcgctcgga tcgacattag ccgtgaccga ggcatccctt
     2101 gcgctcgcgt tcggggcttg cggcgtggcg gtcggagctg gcagcgccgc cgagctctgg
     2161 agcttcgacg gctcgtcagc ggtcgcagcc gtggcgctcg cgagtaagca gcttaccgca
     2221 gtgaccattg cccgacacgc ttggtgcgcg acgctcgacc gcatccgtca gagtagcgcg
     2281 aaaccgccgg ggccgccgcg tgctcacaac ttgctagaaa cgctcgcgat gacctcgcgc
     2341 aaccagcggt gtcccggatc cgcatcgacg ctctcgtgcc acacctgtcc caccttgccg
     2401 accggcagcg ccagtggggg ctttagcagg actagcccga gcgctttcga aaaggcctgc
     2461 gcgacgcgcc gatccaaggc cgcgacgtag tcggtgcgag caaccaccat gggcacggat
     2521 agaaagtggg agacgcgtag ccccacttgt cgccgcaacc cctgcgcggc caacgcccgg
     2581 tcgaccgagc ctggtgagtc gttggaggtc gacacgagca cgtgcgagag ctcgaggtag
     2641 cgcggcagcg tgaggcggtg cttcactctc gggtgtccct tgcgcacgac gcacacgtat
     2701 tcgtccgaga acaggagcgt gtgccggtgc cgcgccggga cgtcgtcgta aaatccgatc
     2761 atcgaatcga attcgccccg cgctagctcc gccgggaccg aatgggaacc ccaggggcga
     2821 atgcgaagcc gcacgcgtgg cgcgatccgc gcgagctcgg cgagtagtgg gggcagtaag
     2881 acgaactcga cgtagtcgct cgcggcgacg gtgaacagtc ggtcgcttcg cgccgggtcg
     2941 aacctctgct cgaccaacgc gctgtcgagc aatttgagcc cacgtccgat tgggtcagcg
     3001 agggcagtcg cgcgcgcggt gggtaacagt cccttcccgg tacgcgtgaa caacgggtcg
     3061 tcgagcaacg cgcggagctg ggcgagagtg ttgctcatgg cgggctgtgt gatgccggct
     3121 cttctcgccg cccgcgtgac gctgcgctcc tcgaagagag cctgaaacgc gacgagcaag
     3181 ttcaggttca cgttgttgat attcattaag tgaatatctc acataattta tatcacttcg
     3241 acattagctc gattgcggcg cagttttctc cttgccggga ccaaatcccg ccgagaaaca
     3301 ggagtgaaac cgatgacgaa catccagacc gaatcgaacc gagaaatttc cgagaacgag
     3361 ctcgcggtgc gcatcgcgag cggccgcgcc ccgctgatcg ccgaaatact cggcccgagc
     3421 tacttcgcgc agggacactt gcccggagcc gtcaatctgc cgctcgaggg tctcgtcgag
     3481 cgcgcgacag ctctcttccc ggaccgaagc gc
//
LOCUS       DASTKS010001178         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1750239, whole genome shotgun sequence.
ACCESSION   DASTKS010001178 DASTKS010000000
VERSION     DASTKS010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750239"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFQ35_25035"
     CDS             complement(<1..418)
                     /locus_tag="VFQ35_25035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934002.1"
                     /translation="MPSGTQFLSVGPNSANPYTLDTSQHFAGKQSLQITKGSGGFYYR
                     ALAVPVPGQNFWVRMYLRVSSTFGDNSHDSLFGASTGALTADSNNETLVEFSEQFNKV
                     LLNTKDQLFQPTDSNTISADMWHCIEAHYDSSSGDVQ"
     gene            405..890
                     /locus_tag="VFQ35_25040"
     CDS             405..890
                     /locus_tag="VFQ35_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934003.1"
                     /translation="MPDGNAVPSNPEQKEASGGHVMSPEPPPATPPPLPPVAVVPPAP
                     PWPPPEDPPRGPPEPPLPALFEEPPEVAPPLCAPPEALPPSPVFVPPELEPEVAPPLF
                     ETPPELLPPLAEAPPLLLPPLVGAAPPLAVLIPAAPLEPPRDEPPVPSFPVLPLLLEH
                     A"
     gene            complement(1182..2054)
                     /locus_tag="VFQ35_25045"
     CDS             complement(1182..2054)
                     /locus_tag="VFQ35_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934004.1"
                     /translation="MLPNAASATTLHDTTTSEPLDAARTSSAVGGPSGGTDAALEPSS
                     TSDSPKSLLDSVALEQRLREHYAKYIDGTRFDFFAVDFAPRVERYLALHDVEVSKLQA
                     EARRFFRDKKHLSFAPKAGSLLVNRETPRTRVQFVLTMAWSQNVSSAFKDCAASDPTL
                     GGDSGPSTIAHKVDVHAELVLDESGRFVSYREIAVVAPRLKVETRSEGVPAFIALPNK
                     PGRSEEPTAPLVPDGTIVEDMGDAFTCSLYGETDTVRKVRVNGKVIWLLAEWTWFTGH
                     NYVGELLLRPVPLP"
BASE COUNT          411 a          726 c          697 g          382 t
ORIGIN      
        1 cctggacgtc gccgctgctg ctatcgtaat gagcttcgat gcagtgccac atgtcggcgg
       61 aaatggtgtt gctgtcggtc ggctgaaaca gctggtcctt cgtatttagc agcaccttgt
      121 tgaactgttc ggagaactcc acgagagtct cgttgttcga gtccgccgtg agcgcgccag
      181 tggaagcgcc gaacaacgaa tcgtggctgt tgtctccaaa cgtggagctt actcgcaggt
      241 acatgcgcac ccagaagttt tgtccaggca ccgggaccgc gagcgcgcga tagtaaaaac
      301 caccgctccc cttcgtgatc tgcaacgact gcttcccggc gaaatgttgc gacgtatcta
      361 gtgtgtacgg attggccgag ttcgggccca ccgacaagaa ctgcgtgccc gacggcaatg
      421 cagtgccctc gaaccccgag caaaaggagg catccggcgg acacgtgatg tcgcccgagc
      481 cgccgccggc aacgccaccg ccgctgccgc ccgtcgcggt tgtaccgcct gcgccgccct
      541 ggccgccgcc cgaagacccg ccgcgaggtc cgcccgagcc gccgctcccg gcgttgttcg
      601 aagaaccgcc tgaagtcgcg ccaccactct gtgcgccgcc ggaggcgctg ccaccgagcc
      661 cggtgttcgt gccgcccgaa ctcgaaccag aggttgctcc gccgctgttc gagacaccgc
      721 cggagctgct tccaccgctc gctgaagcgc ctccactgct gcttccgccg ctggttggcg
      781 ccgcgccgcc gctcgcggtg ctcatccccg cggcgccgct cgagcctccg cgggacgagc
      841 ctcccgtccc atcattccct gtgctcccgt tgctgctcga gcacgcataa agacccatgc
      901 aggcgacgaa gacgccgctg cgccaggtgg atgtcatcga agttgtcggg cgaatggtaa
      961 agctcgaatg ccacatcatg gatgttccgg taacgcgctg cccgagatgt cgcacggctc
     1021 ggcgggatac gcaacgtggc gaccgtgaac atgggggcgg cttcgggcag aatgcctgca
     1081 tttcgagccc ttatcgctcg agagctcccg cgctaaggag ttcggccatg tccgccggct
     1141 cgggcgcacg ttcgccgtgt tgaagcatag cggcgaaatc ctcagggcaa tggaaccggt
     1201 cgaagcaaga gttcgccgac atagttgtgc ccagtgaacc aggtccactc ggcgagaagc
     1261 caaatcacct tgccgttcac ccgcaccttt cggaccgtat cggtctctcc gtatagcgag
     1321 cacgtgaagg cgtcgcccat atcctcgacg attgtaccgt ccggaacgag cggcgccgtg
     1381 ggctcctcgc tgcgcccggg cttgttcgga agggcgatga atgccggaac gccttcgctt
     1441 cgcgtttcga ctttcaaacg aggcgcgacg accgcaatct ctctgtagct cacgaagcgg
     1501 cccgactcgt cgagtacgag ctcggcatga acgtcgactt tgtgtgcgat ggtgctcgga
     1561 cccgagtcgc cgcccagagt ggggtccgac gcggcgcaat ccttgaatgc actcgacaca
     1621 ttttggctcc aagccatcgt caacacgaac tgaacacggg tgcgcggcgt ctcccgattg
     1681 accagcaacg agcccgcctt tggagcgaac gaaaggtgct tcttgtcacg aaaaaagcgc
     1741 cgcgcttcgg cttgcaactt ggagacttcg acatcatgaa gcgcgaggta gcgctcgacg
     1801 cgaggcgcga agtcgaccgc aaagaagtcg aagcgagtgc cgtcgatata ctttgcgtag
     1861 tgctcacgga gccgttgctc gagagcgacc gagtcaagta gcgatttggg tgagtcgctc
     1921 gtcgaagagg gctcgagcgc cgcatccgtg ccaccgctag gtcctccgac agcggacgaa
     1981 gtgcgcgccg catcgagtgg ttcgctcgtc gtcgtgtcgt gcaacgtcgt cgcggacgcc
     2041 gcgttgggga gcatcgagga cggagccggg gccacccccg caacgcgagc gcggtcgtga
     2101 gtcgcgcgtc gctccacggg ctgggaggcc gcgcacccga cgagacccag agcgacgaga
     2161 cgagcgacca gacccagagc gacgagacga gcgaccagac ccagagcgac gagacg
//
LOCUS       DASTKS010001179         4554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_856427, whole genome shotgun sequence.
ACCESSION   DASTKS010001179 DASTKS010000000
VERSION     DASTKS010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856427"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFQ35_25050"
     CDS             <1..858
                     /locus_tag="VFQ35_25050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9934005.1"
                     /translation="FQPDLLLIAVELPTASGLELCGDVRASDPTRHIPTILLGDNADE
                     RAVAAGLLAGADDFVFNLDRMHELRARVHVQLRNKRLFDTLQRVRSERDTLRRDVQID
                     ALTGLLNRRALESGVSERCNSRERFGVLFMDLDHFKTINDRFGHEVGDRVLIAVAHVL
                     KSGLRPGDVVARYGGEEFVALIAGAGPESARLVGERLRMNVEEMPAVPRGPANVTISV
                     GAGVYDPQRAEEAPVELLRRADVALYDAKRAGRNRVVVAAPVGPEPSGPDITQRPPPP
                     QSRLGGARS"
     gene            858..1892
                     /locus_tag="VFQ35_25055"
     CDS             858..1892
                     /locus_tag="VFQ35_25055"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9934006.1"
                     /translation="MASTPGGPSSRVDAPSESPERVASNATALLPTTARAVSEPLIHE
                     SESIVPLPSLMPSGSIPPSIAPLGAMPNDSLPPVAEFALERELVRQLKTGRAALPVLP
                     QVAESALRLANDPDARIDQLARLVDTDPPIAARFMSVANSVAYWRGLVTASTQSAIVR
                     LGLANTRDLLFQVVYAASTNGLKKYQTAVSRSFKASVVAAIAARVTAEETQHRGEYDY
                     MCGLLHNIGEARVYRILDALTLPVPDPALVKRLVQKYHCVAGAEVAMAWKLPTEIVDA
                     CAAHHDAAAAENPSVRLVMIADRIVQALEANALGKPVDYGTFEALGISLELARTLIEK
                     TRKGIGQKGA"
     gene            1963..2625
                     /locus_tag="VFQ35_25060"
     CDS             1963..2625
                     /locus_tag="VFQ35_25060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9934007.1"
                     /translation="MSTHSFGLESAVQSYLVKMASEPEILVALREETAQLPSRGMQIS
                     PEQGALMRLLVGVLGARRCLEVGTFTGYSALSVALALPDDGKVVACDVNQEWTSMAKR
                     YFERAGLLHKFDLRIGPGVATLEELVRGGAADSFDFAFIDADKENYTRYYELCFALVR
                     KSGLIAVDNALWDGKVADASVTDTSTRAIRELNERVRTDSRVRASLVPVGDGLLLAQK
                     LG"
     gene            2735..3946
                     /gene="serB"
                     /locus_tag="VFQ35_25065"
     CDS             2735..3946
                     /gene="serB"
                     /locus_tag="VFQ35_25065"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HET9934008.1"
                     /translation="MNTTAHADLIVLHIAGADRPGVTARLMKIIAEENAELVDIGQSV
                     LHGYLMLSAIVNIPQNSRALRRILFEASDLGLRFEVTPFAPAHADVRADEAPALAVTV
                     LGPLADGAAAARITGFMAERSMNIREIRSLSERELTGLELIADLAPGSDELALDLPAL
                     RGAFLRMGGELGVDLAVQKDDMYRRTKRLVCMDVDSTFVKGEFIDELAELAGCKEKVS
                     EITARAMRGELDFKQALAERVKLLEGLPLERAMTLLDKIELTPGADRFVHALRSLGFR
                     IGLVSGGFTVFVDELKRRYGLDFAFANELEIQGGKLTGRVRGTVVDGERKAQVLKDMA
                     QVYNCRLEQTVAIGDGANDILMLQAAGLGIAFRAKPKLQEVAAMSLNHHERLDMLIHL
                     MGLNARDLRAL"
     gene            complement(3962..>4554)
                     /locus_tag="VFQ35_25070"
     CDS             complement(3962..>4554)
                     /locus_tag="VFQ35_25070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934009.1"
                     /translation="ASRARSAGALPPPATRIEKTVVSPSRTGVPFALEGAAEALAGGG
                     ATGFGGGPMGGCASGRELGAATGARTAAGRGGGEGGSSGTLVASGEGSAEGALDGSAE
                     GAGGGPIGTPPKPSALLAVVAEELGAASETSAARACVAEPSNNSTVNAIRAQCTAFTL
                     SELAPPLQESFGQRLQMQARLAAKAARRPSAPRRQV"
BASE COUNT          737 a         1549 c         1495 g          773 t
ORIGIN      
        1 tttcagccgg atctactgct catagccgtc gagctgccga ccgcgagcgg tctcgagctg
       61 tgcggcgacg tacgggcgag tgatcccacg cgtcacatcc cgaccatttt gctcggcgac
      121 aacgcggacg agcgagccgt cgcggcgggt ctgctcgccg gcgcggacga cttcgtgttc
      181 aacctcgatc gcatgcacga gcttcgcgcg cgcgtccacg tgcagctgcg caacaagcgt
      241 ctgttcgaca cgctgcagcg ggtgcggagc gagcgagaca cgctgcggcg cgacgtgcag
      301 atcgacgcgc tcaccggctt gctcaaccgg cgcgcgctcg agtcgggcgt gtcggagcgt
      361 tgcaactcga gagagcgctt tggagtgctc ttcatggacc tcgaccactt caaaacgatc
      421 aacgatcgat tcgggcacga ggtcggcgac cgcgtgctga tcgcggttgc gcacgtcttg
      481 aagtcgggcc tgcgtccagg cgacgtcgtc gcgcgctacg gtggagagga gttcgttgcg
      541 ttgatagctg gcgccggacc cgaatcagcg cggctcgtcg gcgagcggct ccggatgaac
      601 gtcgaggaaa tgccggcggt gccgcgaggc cccgccaacg tgacgatcag cgtgggcgcg
      661 ggcgtttacg atccgcagcg cgccgaagag gctccggtag agctgttgcg gcgcgccgac
      721 gtggccctct atgatgccaa gcgcgccggt cgcaatcgcg tcgtcgtggc ggcgccggtg
      781 ggtcccgagc cttcggggcc cgacatcacg cagcggccgc ccccgccgca gtcacggttg
      841 ggcggagcga ggtcttgatg gcatctactc ctggcggtcc ttcgtctcga gtcgacgcac
      901 cgagcgagag cccggagagg gtcgcgtcca acgcgactgc gctcttgcca acgaccgccc
      961 gcgccgtgtc cgagccgctg atccacgaga gcgagtcgat cgtcccgctc ccgtcgctca
     1021 tgccttcggg atccatccct ccctccatcg cgccgctcgg ggcgatgccg aatgactcgc
     1081 tgcctccggt ggcggagttt gccctcgaaa gggagctcgt gcgccagctc aagacgggcc
     1141 gcgccgcgct cccggtgctg ccgcaggtcg ccgagtcggc gctgcgactg gccaacgatc
     1201 ccgacgcgcg catcgaccag ctcgcacgcc tcgtcgacac cgatcccccc atcgcggctc
     1261 gcttcatgtc ggtggcgaat agcgtcgcgt actggcgcgg actcgtgacc gcttccacac
     1321 aatcggccat cgtgcggctc gggctcgcga acactcgcga cctacttttc caagtcgtct
     1381 acgccgcctc caccaacggc ctcaagaagt accaaacggc ggtatctcgg tcgttcaagg
     1441 cgagcgtcgt cgcagccatc gccgcgcgcg tgaccgccga ggagacgcag caccgaggcg
     1501 agtacgacta catgtgcgga ctgttgcaca acatcggcga ggcgcgcgtg taccggatct
     1561 tggacgcgct cacgttgccg gtgccagacc cagcgctggt caagcgtctg gtgcaaaagt
     1621 accactgcgt tgccggtgcc gaggtcgcga tggcctggaa gctcccgacc gaaattgtcg
     1681 acgcctgcgc cgctcaccac gacgcggcag ccgcagagaa cccgagcgtt cgcctagtca
     1741 tgatcgcgga ccgcatcgtt caggcgctcg aagcgaacgc gctcgggaag ccggtcgact
     1801 acgggacctt cgaggcactc gggatttcgc tcgagttggc ccgcaccttg atcgaaaaga
     1861 cccgtaaagg catcggccag aagggcgcct gacccgccgt cgcggccgag gcgcagtgtg
     1921 cgagtcctcg tcttcgttcg agccgcggtg taggctccgt ccgtgtccac gcactcgttc
     1981 ggtctcgaaa gcgcggtcca gagctacttg gtaaagatgg cgtcggagcc tgagatcctc
     2041 gtcgcgctgc gcgaggagac tgcccagctg ccgagtcgag gaatgcagat ctctccggag
     2101 cagggcgcgt tgatgcgctt gctcgtcggc gtgctcgggg cccgaaggtg cctcgaggtc
     2161 ggcaccttca ccggttacag cgcactcagt gtcgcgctcg cgcttcccga cgacggcaag
     2221 gtagtggcct gtgacgtgaa ccaagagtgg acgtcgatgg cgaagcggta cttcgaacgc
     2281 gccggtctcc tacacaagtt cgacctccgc atcggtccgg gtgtcgcgac gctcgaggag
     2341 ctggttcgag gcggcgccgc cgattcgttc gacttcgcgt tcatcgacgc cgacaaggaa
     2401 aactatactc ggtactacga gctttgcttc gcgcttgtcc gaaagagcgg gctcattgcg
     2461 gtcgacaacg cgctctggga tggcaaggtc gccgacgcga gcgtgaccga cacgagcacc
     2521 cgagccatcc gcgagctcaa cgagcgcgtc cgcaccgata gccgtgttcg cgcgtcgctc
     2581 gtgccggtcg gggacggcct ccttctggcc cagaagctcg gctgagccgc gccagggccg
     2641 cgaaccaccc gaaaagcctg gggccggcct tggctgcttg cttttcggag attttggacg
     2701 aatcttctcg ccaattcgcc gaggaccttt cgccatgaat acgaccgcgc acgccgatct
     2761 catcgttttg cacattgcag gggccgaccg ccccggcgtt accgcgcggc tcatgaagat
     2821 catcgccgag gagaacgcgg agctcgtcga catcgggcaa tcggttctgc acggctacct
     2881 gatgctgagc gcgatcgtga acatcccgca gaactcgcgc gctctgcggc gcatcctctt
     2941 cgaggcaagc gacctcggcc tgcgcttcga ggtgacaccg ttcgcacctg cgcacgccga
     3001 cgtccgagcc gacgaggcgc ctgcgctcgc cgtgacggtg ctcggcccgc tcgccgacgg
     3061 cgcggctgcg gctcgcatca cgggcttcat ggccgagcgc tcgatgaaca tccgtgagat
     3121 tcgctcgctc agcgaacgtg agctcaccgg gctcgagctc atcgccgact tggcgccggg
     3181 cagcgacgag ctggcgctcg atctgcctgc cttgcgtggc gcgtttctcc gcatgggagg
     3241 tgagctcggc gtcgatctcg ccgtgcagaa ggacgacatg tatcggcgca ccaagcgcct
     3301 cgtctgcatg gacgtcgact ccaccttcgt gaagggcgag ttcatcgacg agctcgccga
     3361 gctcgccggc tgcaaagaga aggtctcgga gatcacggcc cgcgcaatgc gcggcgagct
     3421 cgatttcaag caagcgcttg ccgagcgagt gaagctgctc gaagggcttc cgctcgagcg
     3481 cgccatgacg ttgctcgaca agatcgagtt gacgccggga gccgatcgtt tcgtgcacgc
     3541 gctgcggagc cttggcttcc gcatcggtct cgtcagcggc ggcttcaccg tgttcgtcga
     3601 cgagctcaag cgtcgctacg gcctcgactt cgcgttcgcc aacgagctcg aaatccaggg
     3661 cggaaagctc accggccgcg tgcgtggcac cgtcgtcgac ggcgagcgca aggcgcaggt
     3721 attgaaggac atggcgcagg tctacaactg tcgcttggag cagacggtgg cgattggcga
     3781 cggcgccaac gacattctga tgctccaggc agccgggctc ggcattgctt tccgcgccaa
     3841 gccgaagctt caggaagtgg ccgcgatgag cctcaaccac catgagcgcc tcgacatgct
     3901 catccacttg atgggcttga acgcgcgcga tttacgcgcg ctgtagctca ccgcgcgcgg
     3961 actacacctg cctacgcggc gcactcggtc tcctggccgc tttagcggcg aggcgagctt
     4021 gcatttgaag gcgctgaccg aaactctctt gcagaggcgg ggcgagctcg gagagagtga
     4081 atgccgtgca ctgggcgcgg atcgcattca cggtgctgtt gttgctcggc tccgccacgc
     4141 aggctcgcgc cgcggacgtc tccgaagcgg ctccgagctc ctcggcaacc acggctaaca
     4201 gcgcgctcgg cttcggcgga gtaccgattg gacctccccc ggcgccctcg gccgagccgt
     4261 ccaacgcgcc ctcggccgag ccctctccgg aggcaacgag cgtcccagac gagccgcctt
     4321 cgcctccacc tcggccggcg gcagttcgtg cgccagtcgc ggcgccgagc tctctgccgc
     4381 tcgcgcaacc gcccatcggg cctcctccaa agccggttgc tccgccgcca gcgagcgctt
     4441 ctgccgctcc ctccagcgcg aaggggaccc cagttcggct cggtgagacc accgtctttt
     4501 caatccgcgt ggcgggtggc ggcaaagcac cggcagagcg cgcgcggctc gcca
//
LOCUS       DASTKS010001180         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_409263, whole genome shotgun sequence.
ACCESSION   DASTKS010001180 DASTKS010000000
VERSION     DASTKS010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409263"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFQ35_25075"
     CDS             complement(<1..605)
                     /locus_tag="VFQ35_25075"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9934010.1"
                     /translation="MQRLPASVRVVLVALAVSGAARSSLAADPPSDEALSEARRNEAK
                     AKYQAGVEAYDKHRYKDAVDLFLEADKAAPSAALSFNIGRAYERLGDDTSALRWYRDY
                     LRRSVNAPNAAAVRETVEKLAVALSKKGLQQVTVLSSPAGATVTVDEQAVGVTPWTGE
                     LSPGKHQVLLTLRGYSDGAQEFELRPTEPLDLSLRLEAATPV"
     gene            complement(628..2202)
                     /locus_tag="VFQ35_25080"
     CDS             complement(628..2202)
                     /locus_tag="VFQ35_25080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934011.1"
                     /translation="MALLFTDASEPRYKSGDIIAGKYQLEGLLGEGGMGVVWGAINLQ
                     LEAPVAIKLLSQKSREERELLTQRLKQEAKAAAKLGHPAIVRIFDVGESELGDPFIVM
                     ERLKGRSLAAWLAQEGPMPAILAVRILLPIADALSAAHAKGIVHRDLKPDNVFIDESE
                     QLSPKLVDFGIVKLTDSTSEQTHLTQSGMVLGSPEYMSPEQARGRDDLDARTDVWSFC
                     VVLYEAVSGRTPFAAANSNALLVSIALDDPRPFEAQNEQEAGLWAIIQKGLAKDRALR
                     FQSMVELGRALATWLIAHGVHEDACGASLEAKWIARSTDTSVARHSLATSPVLVVTPP
                     GSAARAPSNELEVAATHAYPGVTVTPSTARERSGTGSGGAGRVRVGLALAGGLLIAAI
                     GAFALSSPARESRVLTLRPAQTPEHIGTQPPQDPVKGLVAAPRDREREHQDVIVEPAA
                     AESVKASEVAAPSVAASAVVVAPSVRPEPSAAASSALVAPRAKAEARAPNGRAARPAK
                     SAEPRERASSDLISPY"
     gene            complement(2476..4014)
                     /locus_tag="VFQ35_25085"
     CDS             complement(2476..4014)
                     /locus_tag="VFQ35_25085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934012.1"
                     /translation="MYPGALATNMPATLGRYELLLPVARGGMGQVWAARLRGARGFNK
                     LVAIKTVLPLPGAEGRLESMLLEEARIAALIHHPNVVQTLELGEYGDTLYLVMEWVDG
                     EPLSFLFERTQEGGSFPLLIAVNLVGQTLLGLHAAHELSDESGAPLGVVHRDVSPHNV
                     LVTHSGVAKLVDFGIAKATKKGTSTTQTGEVKGKFSYMAPEQIRSEAVDRRADIFAAG
                     ILLYTLTTGQHPFKSEHAAALIQSIASDDPAPRPSLSTPGYSRTLEAVVMKALEKDPD
                     ARFHTAAEMLTALQRAMPLAFAPGFEEQVRDFVANLTRERAAQRREALRRAQLAADAR
                     SSPESVSHGSQSPGQSASSLRALIVDTAPTEESQSRASGESTMASPNETRAAAPRRSR
                     SFKSLASVAALLVCAVVLFRTVAQLDVTARRAAASPDMNRTSAHASAPSVPAASQATA
                     SIAAVAPASSTMPSIPATAETAVQPSGSAELRPASTSAKKPLTAANKLHHSRKKTSDL
                     MSPY"
     gene            complement(4356..>4830)
                     /locus_tag="VFQ35_25090"
     CDS             complement(4356..>4830)
                     /locus_tag="VFQ35_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934013.1"
                     /translation="HLSRASACKEWSSRTTMWNQSSTPDDQVLNELCDADSLWGPLVF
                     LRPAREQHFGHGRLLLVCTLFGMFYGMCANAVLAITHHLGGRAVPPVYAVPLFLSLTS
                     FACGEITFLRAWNRRARQLERRRQWEEAKASRPRVVTRPNDKGSDLPTDSHTLTD"
BASE COUNT          842 a         1614 c         1545 g          829 t
ORIGIN      
        1 acaggcgtcg ccgcttccag acgaagtgag aggtcgagcg gctcggtcgg ccgtagctcg
       61 aattcctgag cgccgtctga gtagccccgc aaggtcaaga gcacttggtg tttgcctggg
      121 gaaagctcgc cggtccacgg agtcacgccg actgcctgct cgtcgacggt gaccgtcgct
      181 ccagccggag aggagagcac cgtgacctgt tgcaggccct tcttcgacaa cgcgaccgcg
      241 agcttctcga ctgtctcgcg caccgcggcg gcgttcggtg cgttgacgct tcgccgcagg
      301 taatcccgat accagcgcag agcgctcgta tcgtccccga gccgctcgta ggcgcgacca
      361 atgttgaagg aaagcgcggc gctcggcgcg gctttgtctg cctcgaggaa gagatcgacc
      421 gcgtccttgt accggtgctt gtcgtaagct tcgacgcccg cctgatactt ggccttggcc
      481 tcgttacggc gcgcctcact gagcgcctcg tcgctcggcg ggtcggcggc gagactcgag
      541 cgtgccgcgc cgctgactgc aagcgcgacc aacacgacgc ggaccgaagc gggaagtctc
      601 tgcactgcca acatcctaac gagactctca gtaaggtgag atcaagtcgc tcgaagcccg
      661 ctcacgcggc tcggcggatt ttgccggtcg tgcggcgcgg ccattcggag cacgcgcctc
      721 cgctttggcg cgcggagcga cgagcgcgga gctcgctgcg gcagacggtt cgggccgtac
      781 gctcggcgcg acgaccaccg cgctcgctgc gacagacggc gcggccacct cgctcgcctt
      841 gacgctttcg gcagcggccg gctctacgat tacgtcctgg tgctcccgct cacggtcgcg
      901 cggcgccgcc accagaccct tcaccgggtc ctgtggtggc tgggtgccga tgtgctcggg
      961 ggtttgggcc gggcgcaacg taaggacgcg cgactcgcgc gcgggtgagc tcagcgcaaa
     1021 ggcaccaatc gcggcaatga gcaagccgcc ggcgagagct agaccaacgc gaacgcgccc
     1081 agcgcctccg gacccagtgc cgctacgctc gcgggccgtg ctcggggtta cggtcacgcc
     1141 aggataagcg tgagtggccg cgacttcgag ctcgttgctg ggagctctcg cagcggagcc
     1201 cggcggcgtg accacgagca ccggcgaggt cgcgagggag tgccgcgcca ccgaagtgtc
     1261 cgtcgatcgc gcgatccact ttgcctcgag ggacgccccg cacgcgtctt cgtgaacgcc
     1321 atgcgcaatc agccaggtcg cgagcgctcg cccgagctcg accatcgact gaaagcgcag
     1381 cgcgcgatct ttggcgagcc ccttttggat gatcgcccag aggcccgcct cttgttcatt
     1441 ctgggcctcg aaggggcgtg gatcgtcgag cgcgatgctg accaacaacg cgttggagtt
     1501 ggcggccgcg aagggggtcc gcccgctgac cgcttcgtac agaacgacgc agaacgacca
     1561 cacgtcggtg cgcgcgtcga gatcgtcacg gccgcgggcc tgctccggcg acatgtactc
     1621 ggggcttccg agaaccatcc cagactgggt gagatgcgtc tgctcgctgg tcgaatcagt
     1681 cagcttgacg attccgaagt cgacgagctt gggcgagagt tgctcactct cgtcgatgaa
     1741 cacgttgtcc ggtttgagat cgcggtgcac gatgcctttc gcgtgcgccg cagacagcgc
     1801 gtccgcgatc ggcaagagga tccgcaccgc gagaatcgcg ggcatggggc cctcctgcgc
     1861 gagccacgcg gcgaggcttc tacccttcag ccgctccatc acgatgaacg ggtcaccgag
     1921 ctcggactcg cccacatcga aaatgcgaac aatggcgggg tgtccgagct tcgccgcggc
     1981 cttggcctct tgcttcaaac gttgtgtgag gagctcccgc tcctcgcggc tcttctgcga
     2041 caagagcttg atggcaactg gcgcctcgag ctgcagattg atggcacccc aaacgacgcc
     2101 catgccgccc tcgccgagca gtccttctag ctggtacttg ccggcgatga tgtccccgct
     2161 cttgtagcga ggctcactgg cgtcggtgaa aagcagggcc atcggagcga aacgtctcgg
     2221 aagctagcac aggctgatcg ctcgccgcat ctggcctcat cttggatcga atctcggaac
     2281 tactttctca gcgagccgca tttggggctc ttgggcccca gaaccaaacc agaatttctc
     2341 gagggggcgc gctcatttct gggcccgcat ccgccggacc gcgagctccc gaaaggtcag
     2401 cggctcatct taaaacgagg cctactcgcc cgaggccgtc cagcccggaa agcgcgacgc
     2461 gcaatcttgc ccctttcaat acggcgacat caaatccgag gtcttcttcc gcgaatgatg
     2521 aagtttgttg gccgccgtaa gtggcttttt tgccgacgtc gacgccggtc gcaactccgc
     2581 gcttcccgat ggctggaccg ccgtttcagc cgttgccggg atggatggca ttgtcgacga
     2641 cgctggcgct accgccgcaa tgctcgcggt tgcctggctc gcggccggca ccgacggcgc
     2701 cgatgcgtgc gcggatgtgc gattcatgtc cggtgacgct gcagcgcgtc gagccgttac
     2761 atcgagctga gccacggtcc gaaacaaaac gacggcgcac acgagcagcg ctgccacact
     2821 cgcgagcgac ttgaacgagc gcgatcgtcg cggagctgca gcccgagtct cgttcggaga
     2881 cgccatcgtg ctctcgccgc ttgcccggct ctgcgactcc tccgtcggag cagtatcgac
     2941 gatgagcgcg cgcagagagc tcgcgctctg tcccggtgac tgcgagccat ggctcaccga
     3001 ctccggcgac gatcgagcat cggcagcgag ctgagcgcga cgtaacgctt ctcggcgctg
     3061 cgcggcgcgc tcgcgggtga gattcgcgac gaaatcgcgc acctgttctt cgaagccggg
     3121 cgcgaaagct agcggcatcg ctcgctgcaa cgccgtgagc atctccgccg ccgtgtggaa
     3181 gcgtgcgtcg gggtcttttt cgagcgcctt catgaccacc gcctcgagcg tgcgcgaata
     3241 gcctggcgtc gagagcgatg ggcgcggcgc cggatcgtcg ctcgcgatgc tctggatcag
     3301 cgccgcagcg tgctcactct tgaagggatg ctggccagtc gtcagcgtgt aaaggaggat
     3361 gccggcggcg aagatgtcgg cgcgccggtc gaccgcctcg gagcggatct gctccggcgc
     3421 catgtaggag aactttccct tcacttcgcc cgtctgcgtc gtcgacgtcc ccttcttcgt
     3481 cgccttggcg ataccgaagt ccacgagctt ggcgaccccc gagtgagtga cgaggacgtt
     3541 gtgcggcgac acgtcgcgat ggacgacccc gagcggcgca ccggactcgt cgctgagctc
     3601 gtgcgcggcg tgtaaaccca gcagcgtctg accgacgagg ttcaccgcga tcagaagtgg
     3661 aaaggatccg ccttcttgag tgcgctcgaa caagaaactc agcggctcac cgtccaccca
     3721 ctccatcacc aggtagagcg tgtcgccgta ctcaccgagt tcgagcgttt gcaccacgtt
     3781 cgggtgatgg attagcgccg cgatgcgcgc ctcttcgagc agcatgctct cgagccgtcc
     3841 ctcggctccg ggcagcggta gaacggtctt gatcgcgacg agcttgttga agccgcgcgc
     3901 ccctcgcagg cgcgccgccc acacctggcc catgccccca cgcgccaccg gcaacagcag
     3961 ctcataacga cccaaggtcg ctggcatgtt ggtggcgaga gctccgggat acatcgactt
     4021 acgtgctcga cggttacagc ggacgcgcag agagcgagaa ccgtacggct tccatcctcc
     4081 gatagtcggg aacggctctg ctcggctcga gattacccgt atacgctagc cgacacagaa
     4141 gcagagcacg ccgccgcaag tcgcgcaccg atgcaacgcg cgaccaccgc cgcccgcgcg
     4201 actcgctcgc aaagtgaaac tcgcgggacg cagcatcgcg ccgcgcccgc gtgcttggcc
     4261 gcgaagtgca acccgcgtgg ccctgccaat caccgggcga agtgcgccgc gtcccgaacg
     4321 gcgcagccaa gcgcctctat ctcacttgcg cggcactagt ccgtcagtgt gtgcgaatcc
     4381 gtagggagat ccgatccctt gtcgttggga cgagtcacca cgcgcggacg cgatgctttg
     4441 gcttcctccc actgacgacg acgctcgagc tgacgagcac ggcggttcca ggctcgcaga
     4501 aaagtgatct cgccgcacgc gaagctcgtg agcgaaagga acaatggcac cgcgtaaact
     4561 ggcggcaccg cgcgcccacc caagtgatgc gttatcgcga ggaccgcgtt cgcacacatt
     4621 ccgtagaaca taccgaacag ggtgcaaacc agcagcagac gaccgtggcc gaaatgttgc
     4681 tctcgcgcgg ggcgcaaaaa gactagcgga ccccaaagtg agtccgcgtc acacagctca
     4741 ttcaacactt gatcgtccgg ggtgctgctt tggttccaca tcgtggttcg cgagctccac
     4801 tccttacaag cggacgctcg cgacaaatgc
//
LOCUS       DASTKS010001181         9570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_633125, whole genome shotgun sequence.
ACCESSION   DASTKS010001181 DASTKS010000000
VERSION     DASTKS010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633125"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFQ35_25095"
     CDS             <1..928
                     /locus_tag="VFQ35_25095"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9934014.1"
                     /translation="GGTAIYGASSGDNVFNTANWFLNGSFFTPVGDPLITYRLGRPQK
                     LHYHANDFLKGGAVEPVSISDAEYAALGYASMLGGHRNARGASLIQIRELMRLGLMID
                     VAHMSQETTDDVLVEATRGSGYPIMDSHTGVRADGVVSSDERSLLESQARIIAGLGGV
                     LGIGTATRSDGAAALTSWVRDYSRMRALLGGRGVALGTDLNGLQSQLVSTTVPSRYPI
                     DVAVRRAPAWVISTVPALSQYRLGRRSFEGNRDGIAHYGMLPELFQAVETVSADASSG
                     RLAGTEVVDSLFRSAEDVLRYWEAVQSAAARR"
     gene            complement(1026..1784)
                     /locus_tag="VFQ35_25100"
     CDS             complement(1026..1784)
                     /locus_tag="VFQ35_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9934015.1"
                     /translation="MRHSEPLGCFPTGRHYPGIAHLLRIVAAGLADEFTSDHRVDGLR
                     MVSIDTETTGRDPTVDRIVEIACVTWDGQGIVSRKSWLVNPERPIPQEAFEVHGISDD
                     HVKDKPTFRQIVDEVLAEMEGCIPLAYNAEYDRKVLHSELARLTEPCARKPAAVRKNV
                     EWIDPLVWARELHKHEKSKALTEVSQRLGIAIEQAHRAEHDAEAALRVFVAFAGDTRV
                     PKTYGALLQEQRRLARIFDDEARIWRSRMPEARA"
     gene            complement(1781..2860)
                     /locus_tag="VFQ35_25105"
     CDS             complement(1781..2860)
                     /locus_tag="VFQ35_25105"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9934016.1"
                     /translation="MWSLRVALVAGVATLMSARLTTCRSPGEGGGTEPNPAKSEAADV
                     TLPGVDTSALTTREKAEWSRYVSEFLSPCADQPVSIAECVKQSRPCKACSPAARYVAE
                     LSRRGQVRSQVEAAYKARFSPEAVHTLDLAGSPSKGPANAKVVIAEWADFQCPACKAA
                     APIVEAVLEHHPNDVRLVFKHFPLTIHEHAEKAARASVAALKQNKFWEMHHALFDAQP
                     RVDPENVESLAKGLGLDMKRFAADRDSEATADVVARDRKQGEALALKSTPSIFVNGRQ
                     FVPGGESDDLEEWVKLELELVGASASAPAAPAASNSAPASPGSAPSAAPAASTAPSPS
                     AHTAAAPAASAKPKSAPKPATSAAP"
     gene            2945..4534
                     /locus_tag="VFQ35_25110"
     CDS             2945..4534
                     /locus_tag="VFQ35_25110"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9934017.1"
                     /translation="MSDSVPPSYAPKSSLPPPPGVRHVIAVIGGRGGVGSSCLAINLA
                     VYLAQLGRRVLLIDADPTGAQLHTMLGLELPPLPSSDEDPDEIEIAPLPTSVPGLLLS
                     PQGYRLGSTQPIRPGRKPRWARGIRQLDADYVLFDLGTGTMPSTLDLFLSADQGVCVT
                     TPEPPSIEANYRFLRALFLRALRRSLVEDRFKLRAVERAQAELGALPSPVDLVRAIAR
                     YDTSVGARAAAELAQLRPRLVVNSLRLRTDSELGPAMVDMAVRYLGVKLDYIGQVEQD
                     DAVWLSVVRKRPLLIDSPTSKSARNVERIARRLLALASARAEARPERPAVIIPDEPNL
                     YDVLWTHRGATDEELRRAYKRQREIYQPGSLPLTSLLSEDSLKREQARVEEAHETLLD
                     PLRRKAYDASIFPEDSTRRPPEPQALDAAAEAERMLLRQELAREISAETEFTGRLLRK
                     VREAQGIELEDIAKQTKIALSHLRAIEAETFTELPALVYTRGFVQQLAKYLKLDPTQV
                     TRTFLRRMREARAEPEGDASS"
     gene            4563..6056
                     /locus_tag="VFQ35_25115"
     CDS             4563..6056
                     /locus_tag="VFQ35_25115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9934018.1"
                     /translation="MQVRSAAPRAREAERKGPRSEWNYLLALSLVALLPRLFVALAWS
                     REPVWDGHYYHFGAERIAAGLGYSEDGWAQGHLVWRPWVHYPVGYSGLLAAVYKVFGS
                     RLWVAPLLNALFGTLLAAVSYRIAQTCLSRTRAYVAGALVALHPGLIAYSALVMTELC
                     AALLLSTFGLLLLSFRGRPRGLVIAGLVFGVAVLVRPSSLLLLPIVALLEPSGFVRAL
                     RALAIVGGVGFAVVLPWTIRNCQRFDGCALVSTNGGWNLAIGAISDTGRFQTLRASDG
                     CAVVTGQVQQDRCWAEVGKRRILADPLRFMRLVPKKLAETFDHESFAFEYLHEADPVS
                     WTEPRRVAGRELATGFHRLLMSLAALSVIGLPRGFRRDRVAFGLQLTLLVVAGALSVH
                     AFENDRHPFHWLMLFVLAVGFVPLPGRPPYSPGMLFVLGFLLSVVVTHSLFFGEDRYH
                     MVVSPMLCILAAAALRPAREKQRALAPSPSSDSPAPLVVPSLGRAAP"
     gene            6229..7011
                     /locus_tag="VFQ35_25120"
     CDS             6229..7011
                     /locus_tag="VFQ35_25120"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9934019.1"
                     /translation="MDQARYFVCESCMTPVPSGHKFCGRCGAGVPESVMHLESHFFSD
                     MQDPTKARLILIRGDGMDGLSYHLKADQHIVGRQGQVEFPDDPFVSPRHANFFYRDDR
                     LVVRDEGSLNGVYFRVRGSVEIVPGDLFMAGEQVFRLDPTPRASDAADPDGTFFYSSP
                     KYPSAFRINQILEGGSIGMTVCARGNTLNIGREDGDLNFPGDIFMSGRQSSVEEKDGR
                     FTLTDHDSKNGTYLRIKAEKPLSQGDYVFIGRRLLRVELNAN"
     gene            complement(7187..7567)
                     /locus_tag="VFQ35_25125"
     CDS             complement(7187..7567)
                     /locus_tag="VFQ35_25125"
                     /inference="COORDINATES: protein motif:HMM:NF022443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HET9934020.1"
                     /translation="MRFHKAHFAIAAALLAIESSIALFVHDRFVRPFVGDVLVIPLIY
                     CSIATFFDVRPIKLGLGVFVFACAIEFGQRANLVALLGLEQSRFARTLIGTSYSSLDL
                     VAYAVGAALTVASHLTAQRRNGGP"
     gene            complement(8376..9215)
                     /locus_tag="VFQ35_25130"
     CDS             complement(8376..9215)
                     /locus_tag="VFQ35_25130"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9934021.1"
                     /translation="MPEGDSIFQAAAKLHAALAGQRIVRFFSPLPDLKEAELAGREVT
                     RVYARGKNLIISLDDGRALHTHLRMQGRWRVRQKSAMSEEQRAHLERAPHSLNPVLTL
                     SIETESCIAVCEQAAIAHLVTEREVERRMASLGPDLLAVDFDPKLARDNLRRRPELPI
                     GEAIMLQSMLAGIGNVYKSEVLFLERVSPFVTVAELDDAKLDAIIARSKELLRRNRKG
                     PRRTTFGTLSRMPYYVYERSGELCLKCGERIQMRRQGSLQRSTYYCPECQGVGTRTFG
                     ESR"
     gene            complement(9271..>9570)
                     /locus_tag="VFQ35_25135"
     CDS             complement(9271..>9570)
                     /locus_tag="VFQ35_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934022.1"
                     /translation="DGRLIGYIGRSDRALTTFLTEEEPLRSRELTALTEALRDEVRPG
                     RRAALLIATIDKTAAASSPLASAFVERGFTPSAAGLSLRRAARHLDPMEESEDAE"
BASE COUNT         1545 a         3182 c         3158 g         1685 t
ORIGIN      
        1 cggggggacg gcaatctacg gtgcgtcttc gggcgacaac gtcttcaaca cggccaactg
       61 gttcttgaat gggtcgtttt tcacgcccgt cggcgacccg ctcatcacct accgtctcgg
      121 gcggccgcag aagctccact accacgcgaa cgactttctc aaaggcggag ccgtcgaacc
      181 agtttccatc tccgacgcgg agtacgcggc gctcggctac gcgagcatgc tcggcgggca
      241 tcgcaacgct cggggcgcga gcctcatcca aattcgcgag ctgatgcggc tcgggttgat
      301 gatcgacgtc gcgcacatgt cgcaagaaac gacggacgac gtgctcgtcg aagcaaccag
      361 gggcagcggc tacccaatca tggactccca caccggcgtg cgcgcggacg gcgtcgtctc
      421 gagcgacgag cgctcgctgc tcgagtcgca agcgcgcatc atcgccgggc tcggcggcgt
      481 gctcggcatc ggcaccgcaa cgcggagcga cggcgcggcg gcgctcacgt cctgggtgcg
      541 cgactactcg cgcatgcgag ctctgctcgg cgggcggggc gttgcgctcg ggaccgattt
      601 gaacggcttg cagtcgcaac tcgtgagcac cacggtgccc tcgcgctatc caatcgacgt
      661 cgcggtgcgg cgcgcgcccg cgtgggtcat ctcgaccgtt cccgccttgt cccagtacag
      721 gctcgggcgt cgaagcttcg aaggcaatcg cgacggtatc gcgcactacg gaatgctacc
      781 ggagctcttc caggcggtag aaacggtgag cgccgacgcg agcagcggcc gcctcgcggg
      841 caccgaggtg gtcgactccc tctttcggag cgccgaagac gtcttgaggt attgggaagc
      901 ggtccagagc gcggcggcga ggcgctagcg cctcggaaag cgcgtttatc gcgtcgaccc
      961 cccgggcgct cgagcttcga gttcgggcgt cgccctcggc cgcaaaatga gagttgcggt
     1021 gagagctacg cccgcgcttc cggcatgcgg ctgcgccaga tcctcgcctc gtcgtcgaag
     1081 atgcgcgcca ggcggcgctg ctcttgaagg agggcgccgt acgtcttcgg gacgcgcgta
     1141 tcgcctgcga acgcgacgaa tacacgcagc gcggcctcgg cgtcgtgctc ggcgcgatgc
     1201 gcttgctcga tcgcaatgcc gaggcgttgg ctcacctcgg tcaacgcctt gctcttctcg
     1261 tgtttgtgaa gttcacgcgc ccacacgagc ggatcgatcc actcgacgtt cttacggacg
     1321 gcggcgggct tgcgcgcgca cggctcggtg agacgcgcga gctcggagtg cagcaccttt
     1381 cggtcgtact cggcgttgta ggcgagcggg atacagccct ccatctccgc cagcacctcg
     1441 tcgacgattt ggcggaaggt gggcttgtcc ttcacgtgat cgtcgctgat accgtgcacc
     1501 tcgaacgcct cttgggggat cgggcgctcg gggttcacta gccagctctt gcggctgacg
     1561 atgccctggc cgtcccaggt gacgcacgca atttcgacga tacgatcgac ggtgggatcg
     1621 cgcccggtgg tctcggtatc gatcgagacc atgcgcagcc cgtcgacgcg gtgatccgaa
     1681 gtgaattcgt cggccaaacc cgcggccacg atgcgcaaga ggtgagcgat gccggggtag
     1741 tgacgaccgg taggaaaaca gccgagtggt tcgctatgcc tcatggtgca gcgctggtgg
     1801 caggcttcgg cgcagatttc ggtttcgcgc tcgcggcggg ggcggccgcg gtgtgagcgc
     1861 tcggtgacgg agcggtgctc gcggcgggag ctgcgctcgg cgccgaaccg ggagaggcag
     1921 gcgcagagtt gctcgcggcg ggagctgccg gcgcggaggc agacgctcca acgagctcga
     1981 gctcgagctt gacccactct tccagatcgt cggactcgcc acccggtacg aactgccgac
     2041 cattgacgaa gatggagggc gtgctcttca aagcgagcgc ctcgccctgc ttgcgatcgc
     2101 gcgcgacgac gtcagccgtc gcttcgctat cgcggtcggc ggcgaagcgc ttcatgtcga
     2161 ggccgagtcc cttcgcgagc gactcgacgt tctccggatc gacgcggggc tgcgcgtcga
     2221 acagggcgtg gtgcatctcc caaaacttgt tttgcttcag cgccgcgacc gatgcgcgag
     2281 ccgctttttc ggcgtgctcg tggatggtca ggggaaagtg cttgaacacg aggcgcacgt
     2341 cgttggggtg gtgctcgagc acggcctcga cgatgggggc tgcggctttg cacgctgggc
     2401 attgaaaatc ggcccattcc gcgatgacga ccttcgcgtt cgcggggccc ttcgagggag
     2461 aacccgcgag gtcgagcgtg tgcaccgcct cgggagagaa gcgcgccttg taagccgctt
     2521 cgacctgcga gcgcacctgg ccgcggcgcg agagctcggc gacgtaacga gctgccgggc
     2581 tacacgcctt gcacgggcgc gattgcttca cgcactccgc gatgctgacc ggctgatcgg
     2641 cgcaggggct caggaactcc gacacgtagc gagaccactc ggccttctcg cgcgtggtca
     2701 aagcggaggt gtccacgcct ggtagcgtca cgtccgcggc ctcgctcttc gccgggttcg
     2761 gttcggtccc accgccttca cccggactgc ggcaagtcgt cagccgggcg ctcatcaagg
     2821 tggccacgcc ggccaccaaa gctacacgca agctccacat cggggccgag cctagccgag
     2881 cccaagcgcc ttggcgactg gtgcggcgcc cccgaacgat cgtggtaata ggacccccgg
     2941 tccgatgtcc gacagcgtcc cgccgtccta cgcgccaaag agcagcctgc cccctccccc
     3001 cggagttcgt catgtcatcg ccgtgatcgg cgggcgcggc ggggtcggct cgagctgcct
     3061 cgccatcaat ttggccgttt acctggcgca gctcggtcgg cgcgtcctgc tcatcgacgc
     3121 cgacccgacc ggggcgcagc tgcacacgat gctcggcctc gagctgccgc cgcttccgag
     3181 ctcggacgaa gatccggacg agatcgaaat cgcgcccctt ccgacgtcgg tccccggcct
     3241 cctcttatca ccccaggggt atcgcctcgg ctccactcag ccgatacgcc ccggacgaaa
     3301 gccgcgctgg gcacgtggca tccgtcagct cgacgccgac tacgtgctgt tcgatctcgg
     3361 caccggcacc atgccttcga cgctcgatct gtttctctcg gcggatcagg gcgtgtgcgt
     3421 cacgactccc gagcctccgt ccatcgaagc taactacagg ttcctgcgcg cgctcttctt
     3481 gcgagctctg cggcgcagct tggtagaaga tcgcttcaag ctgcgcgcgg tcgaacgcgc
     3541 tcaggccgag ctcggcgcac tgccgtctcc ggtcgatctc gtccgcgcga tcgctcgcta
     3601 cgacacgagc gtcggggcgc gcgccgcagc agagctcgcg cagctccgac cccggctcgt
     3661 cgtcaacagc ttgcgcctgc gcaccgattc ggagctcggc cccgcgatgg tcgacatggc
     3721 ggtgcgctac ttgggcgtaa agctcgacta tatcgggcaa gtcgagcagg acgacgccgt
     3781 ttggctcagc gtcgtgcgga aacgcccgct cctcatcgat agcccgacca gcaagagcgc
     3841 ccgcaacgtg gagcgcatcg cgcggcgttt gctcgcgctc gcttcggcgc gcgccgaagc
     3901 acggccggag cgtcccgccg tgattatccc cgacgagccg aacctctacg acgtgctgtg
     3961 gacgcatcgc ggcgcgacgg acgaggagct ccgccgcgcc tacaagcgcc agcgcgaaat
     4021 ctatcagccg gggagcttgc ccctcacctc gcttctctcc gaagactctc tgaagcgcga
     4081 gcaagcgcgc gtggaagagg cgcacgagac gttgctcgac ccattgcgcc gcaaagccta
     4141 cgacgcctcc atcttccccg aagactcgac gcggcgtccc cccgagccgc aagcgctcga
     4201 cgccgctgcc gaagccgaac gcatgctgct ccgacaagag ctcgcgcgcg agatcagcgc
     4261 cgagaccgag ttcaccggcc gcttgctgcg caaggtgcgc gaagcgcagg gcatcgagct
     4321 cgaggacatc gccaagcaga ccaagatcgc gctctcgcac ctgcgcgcga tcgaagcgga
     4381 gacgttcacg gagctgcccg cgctcgtgta cacgcgcggc ttcgttcagc agctcgcgaa
     4441 gtatttgaag ctcgatccca cgcaagtgac gcgcacgttc ttgcggcgta tgcgtgaggc
     4501 gcgtgccgaa cccgagggag acgcctcttc gtgaagcggt ctcgaggaga cgccgggacg
     4561 tgatgcaggt ccgatcggcg gcgccgcgtg cccgcgaagc cgaacgaaag ggcccgcgaa
     4621 gcgagtggaa ctacctactc gcgctctcgt tggtggcgct cttgccacgc ctcttcgtcg
     4681 cgctcgcttg gtcccgagag ccggtttggg acggccacta ttatcacttc ggcgccgagc
     4741 gcatcgccgc agggctgggc tactcggaag atgggtgggc gcagggccat ttggtgtggc
     4801 ggccctgggt gcactacccg gtcgggtaca gcgggctgct cgcagccgtt tacaaggtgt
     4861 ttgggtctag gctttgggta gctccgctcc tcaacgccct gttcggcacc ttgctcgcgg
     4921 ccgtgagtta ccgcatcgcg cagacatgtt tgagccgaac ccgggcgtac gtggccggcg
     4981 cgttggtcgc tcttcacccc ggcctgatcg cctacagcgc gctcgtcatg accgaactct
     5041 gcgcggccct gctgctctcg acgttcgggc tcttgctgct gagtttccgg ggacgtccgc
     5101 gcggactcgt gatcgcgggg ctcgtgttcg gcgtcgcggt gttggtgcgc ccgtcgtcac
     5161 tgctgctgct ccccatcgtc gcgttgctcg aaccttccgg tttcgttcgg gcgctgcgcg
     5221 cgctcgccat cgtcggcgga gtcgggttcg cggtggtgct gccgtggacg attcgcaact
     5281 gccaacgctt cgacggctgc gcgctcgtgt cgaccaacgg cggctggaac ctggcgattg
     5341 gcgcgatcag cgacaccggt cgcttccaaa cgttgcgcgc ctcggatggc tgcgccgtgg
     5401 tcacggggca ggtgcaacaa gatcgctgct gggccgaggt cggaaagcga cgtatcctgg
     5461 ccgacccgct gcgcttcatg cggctcgtcc cgaagaagct cgcggagacg ttcgaccacg
     5521 agtcgttcgc gttcgagtac ctgcacgagg cggatccggt gagctggaca gagcctcgcc
     5581 gcgtcgcggg ccgagagctc gcaaccggct ttcatcgatt gttgatgagc ctcgccgcac
     5641 tttcggtgat cggtttgccg cggggcttcc gtcgcgatcg agtcgcgttc gggctgcagc
     5701 tcaccctgct cgtcgtagcg ggcgccctga gcgtgcacgc cttcgagaac gaccgtcacc
     5761 catttcattg gctgatgctc ttcgtgctcg cggtgggctt cgtgccgcta ccgggcaggc
     5821 cgccctattc accgggcatg ctgttcgtgc tcggcttcct cctcagcgtc gtcgtcactc
     5881 attcgctctt cttcggcgag gaccgctacc acatggtcgt aagcccgatg ctttgcatcc
     5941 tcgccgccgc cgcgctgcgc cccgcgcgcg aaaagcagcg agcgctggct ccgagcccga
     6001 gctctgacag ccccgccccc ctggtcgtcc cgtcgctcgg gcgcgcggcc ccgtaacggc
     6061 tcgccaaccg gtaacggcca tcggccgggc aagccgggcc ggccaaattt cgcttgaaat
     6121 cgcggatcta gccatcaagg tcgttcccaa gagctgaaaa ttgttggacc ctcgtccctt
     6181 gcccccgccc ggtgagcccc ggcggccgcg gcgccccagg agagcctcat ggaccaagcc
     6241 aggtacttcg tatgtgagtc gtgcatgacc cctgtcccga gcggtcacaa gttctgcggc
     6301 cgctgcggcg ccggcgtgcc ggagagcgtc atgcacctcg agtcgcattt cttcagcgac
     6361 atgcaggacc ccacgaaggc gcgcctcatc ttgattcgcg gcgacggcat ggacgggctc
     6421 tcctaccacc tgaaggcgga ccaacacatc gtaggccgcc aggggcaggt cgagttcccg
     6481 gacgacccct tcgtctcgcc gcgccacgcg aacttcttct accgtgacga ccgcctcgtc
     6541 gtgcgcgacg aaggctcgct caacggcgtc tacttcaggg tacgcggctc ggtcgaaatc
     6601 gtacccgggg atctcttcat ggctggcgag caagtgttcc gcctcgatcc cacgccgcgc
     6661 gcctccgacg ccgccgatcc cgacggcacc ttcttctatt cttcgccgaa gtacccgagc
     6721 gcgttccgca tcaaccaaat cctcgagggc gggagcatcg gcatgaccgt gtgcgctcgc
     6781 ggcaacaccc tcaacatcgg acgcgaggac ggcgatctca acttccccgg cgacatcttc
     6841 atgtccggcc gccagagcag cgtcgaagaa aaagacggcc gcttcacgct caccgaccac
     6901 gacagcaaga acggcaccta cctgcgcatc aaggcagaaa agccgctctc gcagggcgac
     6961 tacgtgttca ttggtcggcg cctgctgcgc gtcgagctca acgcgaactg agccggcgaa
     7021 gaaacgcttt cgcgtcggcg cgccgagcgc actggaatcg atcgctgcga agcgaccttc
     7081 caggccaaga tgatacattt cgggcaccga cgctggctcg cgggagggct tgtggtggtg
     7141 gtgttggcgc tcgtctcggc gcgctggctc gggatgggcg acgaagctaa gggccgccgt
     7201 tccgacgttg cgccgtcaga tgcgacgcta cggtcagcgc cgcgccaacg gcataggcga
     7261 ccaagtcgag agacgaatac gacgtcccga tcagcgttcg cgcaaagcga ctctgttcga
     7321 gaccgaggag ggcgacgaga tttgcgcgtt gaccgaactc gatggcgcac gcgaaaacga
     7381 acacgccgag cccgagcttg atcggccgca cgtcgaagaa cgtcgcgatc gagcagtaga
     7441 tcaaggggat gaccagaaca tccccgacga acggacgcac gaagcggtca tgcacgaaca
     7501 gtgcgatcga cgattcgatc gcgaggagcg cggccgcgat agcaaagtgc gccttgtgaa
     7561 atcgcacgct agctcccgtc cctcacccag cttggtcttg tacgctcgtg catgctcact
     7621 tcgtaccatt cccgagcacc tgatcgttac ttcgaggtgt cgtggtcggt gctattctcg
     7681 aggccatggc gaatgacctt ggcgtcagtg ggaggcgagc gccgtggttc gaatcagcag
     7741 ccaatcgcgc gctgatctcg ttagctgccc tcacctgcat cgcgatcgtt tgggggtgcg
     7801 gcaacgatgg cgcgactggc agcgaccgca attcgggcgg ggcaagtcat ttgggaggct
     7861 cgaccagcgc tggtgggcgg ccgagctccg gcggagcaac cggctcgggt ggagacgcgg
     7921 actccggtgg tcgcgaaccc gactcgaggg gctacgccat cgttgggctg gacctctatc
     7981 ccccactttt cgagcgcgtg ctcgcgttcg actgccaacc tggctccgca ggctcatcgg
     8041 agtgtttatc ggacagcgat tgtgggacgg gcatggcctg tgtgtgcggc gggttactgg
     8101 gagccgacgc gcggaaccga tgcatgcccg cagagtgccg aaccggcgcc gattgcgggg
     8161 aaggcagctg tctactcagc ctgggcagcg caatcgatga ctgctgcacg tttgggaacg
     8221 cgggactatt ttgccgccgc aaggacagca cctgccaaag tgggggagat tgcccgggca
     8281 acgggcaagc ctgtatctat actttcgcta gcgaccgctt cgaatgtcag cgctcatcgt
     8341 gcatttgcgg aggttgagct ggcaaagccg atcgctcagc gcgactcgcc aaaggtcctg
     8401 gtgccgacac cctggcactc ggggcagtaa taggtggagc gctggagcga accctgacgg
     8461 cgcatctgga ttcgctcgcc gcacttgagg cagagctcgc cgctgcgctc gtaaacgtaa
     8521 taaggcatgc ggctcagggt tccgaaagtc gtgcgcctcg gacctttgcg gtttcgccgc
     8581 aagagctcct tcgagcgcgc gatgatggcg tcgagcttcg cgtcgtcgag ctcggcgacg
     8641 gtcacgaacg gcgatactct ctctaaaaag agcacctccg acttgtacac gttgccgatc
     8701 cccgccagca tcgattggag catgatcgct tccccgatcg gcagctcggg gcgccttcgc
     8761 aggttgtccc gcgcgagctt cgggtcgaag tccaccgcga gcaaatcggg cccgagcgag
     8821 gccatgcgcc gctcgacctc gcgctcggtg acgagatgag cgatcgccgc ctgctcgcac
     8881 acggcgatgc agctctcggt ttcgatcgaa agcgtgagca cgggattcag agagtgggga
     8941 gcgcgttcga ggtgcgcgcg ctgttcttcg gacatcgcgc tcttctggcg cacacgccag
     9001 cgtccctgca tgcgcaagtg agtgtgcagc gcccgaccgt cgtcgagcga gatgatgaga
     9061 tttttgccgc gcgcgtagac gcgcgtcact tcgcgcccgg cgagctcggc ttctttcaag
     9121 tctggcagcg gagagaagaa gcgcacgatg cgttgaccag cgagcgcggc gtgaagcttc
     9181 gcggcggctt ggaatatgga gtcgccttcg ggcattcagc gctcgctcgc gcgctccaat
     9241 gcgctggccg cgcctgagag cacgctgtgg ctactcggca tcttcgctct cctccatcgg
     9301 gtcgaggtgc cgcgcggctc tgcgcaaaga gagccccgcc gcggagggag tgaacccacg
     9361 ctcgacgaaa gcgctcgcga gcggggagct cgccgccgcc gtcttgtcga tggttgcgat
     9421 caagagggcc gcgcgccgcc ccggccggac ttcgtcgcgc agagcctcgg ttagcgcggt
     9481 gagctcgcgg gaacggagcg gctcttcttc ggtcaagaag gtggtgagcg cgcgatcgct
     9541 tcggccaatg tagccgatga gcctgccgtc
//
LOCUS       DASTKS010001182         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_409690, whole genome shotgun sequence.
ACCESSION   DASTKS010001182 DASTKS010000000
VERSION     DASTKS010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409690"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..898)
                     /locus_tag="VFQ35_25140"
     CDS             complement(200..898)
                     /locus_tag="VFQ35_25140"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET9934023.1"
                     /translation="MAGFSIDIWSDVACPWCYIGKRRLEAALPQVPGLGEVEITWHAF
                     ELDPSAPRENNSGTSYAERLAKKYGQTTDQAERMIARVVEVARAEGLDFRFDRIRPGN
                     TFDAHRLLAHARLAGRQDAVAERLFHAYFTEGKCIARAGELQSVAVAAGLDADVVSNL
                     LSSDLLTEEVRQDQSEAAALGVHAVPFFVFDQRLAVSGAQGTTVFVQALERALRERAP
                     SEFEEGAVCGPAGC"
     gene            1025..1531
                     /locus_tag="VFQ35_25145"
     CDS             1025..1531
                     /locus_tag="VFQ35_25145"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9934024.1"
                     /translation="MNSMSRVNDVMHSPLQIVPADMQAVDALQFAERCGVHHLPLRDR
                     DQIIGLVCTCDLEELDLKAPIRNAVKRELATIPSDSGLEEAARVMSREEVGSLLVTDH
                     GSMIGIVTREDLADAGADVGAIPHFRCESCGAIRHLKRDGERGTLCLDCRTRAEPEVP
                     GDGTGVGD"
     gene            complement(1685..>2279)
                     /locus_tag="VFQ35_25150"
     CDS             complement(1685..>2279)
                     /locus_tag="VFQ35_25150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934025.1"
                     /translation="KVARLPDEAKPLGEQLLSRKHEIRRSIDRVLRVPLTGLSMRVHG
                     DYHLGQVLFTGRDFYIIDFEGEPARSINDRKRLRSPLVDVAGMIRSFHYAAFGALTQD
                     VPGSQVRPEDRAALAPWAHAFYRASARCFWESYLKEIEPLGVLPRDRSERALLLEVHL
                     LEKALYELVYELNNRPNWAELPLRGILSLLDATKSQS"
BASE COUNT          416 a          720 c          747 g          396 t
ORIGIN      
        1 ccaggcgtag cgtctcgagc cagctcccga cgagcggcgc aagcgcggag gttggggacg
       61 gggctccctt cggttcaggc gtcgttccgc tcactcgagc ggtggccttg ggcgtgtcac
      121 ccggccgtcg atggcacccc atacccgcca gcgtcaggag cacgagactg aaggctcgcc
      181 gcacgtattt cgagcccctt cagcacccgg ccgggccgca gacggctcct tcttcgaact
      241 ccgagggagc gcgctcccga agcgcgcgct cgagcgcctg aacgaacacg gtggtcccct
      301 gcgcgccgga aacggccaga cgctggtcga atacgaagaa cggcacggcg tgaacgccga
      361 gagctgcggc ttcgctctgg tcttggcgga cctcttcggt gagcaagtcg ctcgagagta
      421 agttgctcac gacgtcggca tcgagcccgg ccgcgacggc aaccgactgc agctcgccgg
      481 cgcgagcgat gcactttccc tccgtgaagt acgcgtgaaa caaacgctcg gcgaccgcgt
      541 cttgtcgccc cgcaagcctt gcgtgcgcaa gcagtcgatg cgcgtcgaaa gtgttaccgg
      601 gacggatgcg gtcgaaacga aagtcgaggc cctcggcgcg cgcaacctct acgacacgag
      661 cgatcattcg ctccgcttgg tcggtagtct gtccgtactt ctttgcgaga cgttcggcgt
      721 aagaagtgcc agaattgttt tcacggggcg cgctcgggtc gagctcgaac gcgtgccacg
      781 tgatttctac ctcacccagg ccggggacct gtggcaacgc cgcttcgagc cggcgctttc
      841 cgatgtagca ccagggacaa gcaacgtcgg accagatatc gattgagaag ccagccaccc
      901 cgcagaactt agggcccgag cagtgccaac gcaatcggct cggcctttcg attttcggaa
      961 tgattcagcc gatttcgcga aagctggcac ggaccttgta cgcatttcaa ccgaggagga
     1021 ccccatgaac tcgatgtcac gagtcaacga tgtcatgcac tcaccgctcc aaatcgttcc
     1081 ggcggacatg caagccgtcg atgcgctgca gttcgcggag cgctgcggcg ttcatcacct
     1141 accgttacgc gaccgcgacc aaatcatcgg tttggtgtgc acctgcgacc tcgaggagct
     1201 cgacctcaag gcgccgattc gaaatgcggt aaagcgtgag ctcgctacga ttccgtccga
     1261 ttcggggctc gaggaggcag cgcgcgtcat gtcgcgggaa gaggttggct cgctgctcgt
     1321 gacggatcac ggatcgatga tcggcatcgt cactcgcgaa gatttggctg atgcgggagc
     1381 cgacgtcggg gcgattccac acttccgctg cgaaagctgc ggcgccattc ggcacctgaa
     1441 acgcgacggt gagaggggaa ccctttgcct cgactgcagg acgcgcgccg agccagaggt
     1501 ccccggcgac ggcaccggcg taggtgactg aagatcgcga tcgagctccg tgcgcggcac
     1561 ccccgatcgg gcgggtgccg gcacgtcgag tcacactgac tacccgttgg cgagcttagg
     1621 cggagcccgt ccgccgcagc aagaggattg gctcgcgcat tatgacctac ccattcgcgg
     1681 gcccttagga ttggctcttc gtcgcgtcga gcagagagag aatgccgcga agcggtagct
     1741 cggcccagtt cgggcggttg ttgagctcgt agacgagttc gtagagagcc ttttcgagca
     1801 agtgcacttc gagcagcagg gcgcgctcgc tgcgatcgcg tggcagcacc ccgagcggct
     1861 cgatctcttt caagtagctc tcccaaaagc agcgtgcgct cgcgcggtag aaggcgtgag
     1921 cccatggggc gagcgcggcg cgatcctctg ggcgcacctg agagccgggg acgtcttgag
     1981 tcagcgcgcc gaacgcggcg taatggaacg agcgaatcat gccggcgacg tccacgagcg
     2041 gcgagcgcaa gcgcttacgg tcgttgatgc tgcgcgctgg ctcgccctcg aagtcgatga
     2101 tgtagaagtc gcgtccggtg aagagcacct gtccaaggtg atagtcgccg tgcacgcgca
     2161 tgctcagccc cgtgagcggc acccggagaa cgcggtcgat gctccgccga atctcgtgct
     2221 ttcggctgag gagctgctca ccgaggggct tcgcttcgtc gggcagccgc gcgaccttc
//
LOCUS       DASTKS010001183         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1976802, whole genome shotgun sequence.
ACCESSION   DASTKS010001183 DASTKS010000000
VERSION     DASTKS010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976802"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..1310
                     /locus_tag="VFQ35_25155"
     CDS             525..1310
                     /locus_tag="VFQ35_25155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934026.1"
                     /translation="MNTKAAGGYAGAIGNHSQAQNRASFNFGPRRSSLAVGAGGFGVV
                     ALLAFVAWRSTSLASPSKRDGDSRLSELQSSARANERAIAQLEQELRATKLAAAHAES
                     NPKHAASEVPPIASAAVAQPERAPEPAAPPHTPAQENQFFGDYFAELDTMMQHEGVDV
                     AWSREISKVVQEAYGRQNLQGSKLTRIECGKTLCRVESEHASEKARELFKMTFVGSLG
                     GRFERFTLHAPMDALTTTTFMAKTGAVLPSHRDLGIPFPGEPE"
     gene            1379..1846
                     /locus_tag="VFQ35_25160"
     CDS             1379..1846
                     /locus_tag="VFQ35_25160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934027.1"
                     /translation="MRMCGMAVAALASALLLAGTADAAIVYGANFCKAADYDFAAPSF
                     NTENQIIVDGPSNKWAYLSCPMVNTKNHTTGLAAGSVRLNSSSTTSYCLMLSSTEFGS
                     ILASQTTYVTTTNTDVSLNVYSGVNSSFSYGYFNAFCVLSGGSRMMSYRYLEQ"
     gene            1882..2343
                     /locus_tag="VFQ35_25165"
     CDS             1882..2343
                     /locus_tag="VFQ35_25165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934028.1"
                     /translation="MKQFTLSALVAGVALLAGQAHADLVFPGGACATAIDHDFPVVLD
                     GTASIGGDASEDVVLTCPVPKTNNSTAGIASGTVRVRATNANLSSCAMRALTQYGSVL
                     SGQSVAITTVNQPVYLNVGDSVTAGAMYGYYALSCALSGGSRLYGYRYREN"
     gene            2414..2881
                     /locus_tag="VFQ35_25170"
     CDS             2414..2881
                     /locus_tag="VFQ35_25170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934029.1"
                     /translation="MKLSLLSAAFAAALLLSGQASALMIFGGNACNPLGGSADLDYYR
                     LDGGFYVLGANNAFSYLSCPMANMTNQLNGLESGWIRFRVTNNQSSCSMRSMTAWGSV
                     VSGSAVTLTTLNSDHSLNIGANVTSSNSNGFFTLGCTLSGGSRLYSYRYEEAP"
     gene            complement(2993..>3752)
                     /locus_tag="VFQ35_25175"
     CDS             complement(2993..>3752)
                     /locus_tag="VFQ35_25175"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9934030.1"
                     /translation="VNCGALDRALVRSELFGHARGAFTGASDAHAGVFEQASGGTLFL
                     DEVAELPLDVQPMLLRALEVGTVTRLGENRERPVDVRVISATHRALDQLVQEGVFRED
                     LFYRLVVATVHVPPLRERPEDIPVLVRHFAEEMASSEVPPEVVTRWLQRTWPGNVREL
                     KNAVRSYIALGGFEQPSRSPDARAPDLQLALQHFVDIERRYADLKEDLLERFVVVYLE
                     HLLRHTQGNQSEAARLSGIERSHLNRMLKRVRPK"
BASE COUNT          688 a         1197 c         1178 g          689 t
ORIGIN      
        1 gaggggaaac tcgaggcagc ggaacgcgca taccggacgg ctttgcagaa gaaccccgct
       61 ttgcccgggc tcgagtggaa cctcgaagtg ttgctcaccg aacgggcaaa agccggctga
      121 gcatcaagct gtcccgccgt cgcaataccg ggggtcacgg agagggtgcg ctcgttcgca
      181 cagttgtgtc gcgctgcgca cacccattcg tcgccgagcg tcgcgccgac tcgccgcgcg
      241 aatcccgttg ggctcgcgat gctcgtcgtg gctcgagcct gtgcgctcga tgacactcac
      301 gaggacctgc cctctctagc caagtcgcgg ctgccggggg gcgcggttag cgagcacagc
      361 tgacgagtct tgggaaattt ccgagctgtc gacggcattc gctcgaatct cgcgggctct
      421 cagtcacgcc caatcagtgc ctccaatgga gcgaggcacg cgccttgctt tggcggcggg
      481 ctcgttggcg cagagcgcgt cacccaagga gctcgcgatc acccatgaat acgaaggcag
      541 caggcggata cgctggagcg attggcaatc actctcaagc gcagaatcga gcatcgttca
      601 atttcgggcc gaggagaagt agcctcgccg tgggtgcggg cgggttcggt gtggtcgcgc
      661 tactggcttt cgtcgcctgg cgctccactt cgctcgcttc gccaagcaag agggacgggg
      721 actcccgcct gagtgagctt caatcgagcg ctcgcgcgaa cgagcgcgcc atcgcccagc
      781 tcgagcaaga gctccgggct accaagcttg ccgccgcgca cgccgaatcc aacccgaagc
      841 acgctgcctc cgaggttccg ccgattgcga gcgcggctgt ggcgcaaccc gaacgcgctc
      901 cggagcccgc agcgccgccg cacacgccgg cgcaagaaaa tcagttcttt ggcgactact
      961 tcgccgagct cgacacgatg atgcagcacg agggggtcga cgtcgcctgg tcccgggaga
     1021 tctcgaaggt cgtccaggag gcgtacgggc gacagaacct ccaagggtcc aagctgacgc
     1081 gcatcgagtg cggaaagacg ttgtgccgcg tcgagagcga gcacgcgagc gagaaggcac
     1141 gcgagctgtt caagatgacg ttcgtgggct cgctgggcgg gcgattcgag cgctttacgc
     1201 tgcacgcccc catggacgca ctgacgacga cgacgttcat ggcgaagacg ggggcggtgc
     1261 ttccttcgca tcgcgatctc ggtatcccat ttcccggtga acccgagtga gcagcaccgc
     1321 gtcgcgtacg gcgagaccag cccttactcc atcgattgat aagaaaggac cgatacccat
     1381 gagaatgtgt ggaatggcgg tagcggcgct cgcgagcgcc ttgttgctcg cggggactgc
     1441 agatgccgcg atcgtgtacg gcgcgaactt ctgcaaagcc gccgactatg atttcgcggc
     1501 gccgagcttc aatacggaga atcaaatcat cgtcgacggg ccgagcaaca agtgggcata
     1561 cctcagctgt ccgatggtca acaccaagaa tcatacgacg gggctcgccg cgggctccgt
     1621 tcgcttgaac tcgtcgagca cgacctctta ttgcctgatg ctctcttcga ccgagttcgg
     1681 ctcgattctg gcttctcaga cgacctacgt tacgaccacc aatacggacg tgtcgctcaa
     1741 cgtgtatagc ggcgtcaact cgagcttttc ctacggctac ttcaacgctt tctgtgtctt
     1801 gagcggtggc agtcggatga tgtcgtaccg ctaccttgaa caatgagctc ttttcctgga
     1861 tccaaccgag aaggaaactc catgaagcaa tttactctgt cggcgctagt cgccggcgtc
     1921 gcactcctcg ctggacaggc ccacgcagat ttggtgttcc ccggcggtgc ctgcgccacc
     1981 gctatcgatc atgatttccc cgttgtgctg gacggcaccg cgtctattgg gggagatgcc
     2041 tccgaggacg tcgtgctgac gtgtcccgtc cccaagacca acaactcgac tgccggcatc
     2101 gcgagcggca ccgttcgtgt gcgcgccacg aacgcgaacc tgagtagctg cgccatgcgc
     2161 gcgctgacgc agtatggatc agtgctctcg ggccagagcg tcgcgattac gacggtgaat
     2221 cagcccgttt acctgaatgt cggagatagc gtgaccgcgg gcgccatgta cgggtattac
     2281 gctctcagct gtgctctcag cggggggagc cgcttgtacg gatatcgcta ccgcgaaaac
     2341 tgagcccgcg cagcggcttc cctagcccac ccatcctccg caccctttac cttcgtgtga
     2401 aagacatcga atcatgaaac tctctcttct ttcggcggcg ttcgccgccg cgctgctgct
     2461 ctccggtcag gccagcgcgc tgatgatctt cggaggcaac gcctgcaacc ccctcggtgg
     2521 ctcggccgac ctcgactatt atcgactcga cggcgggttc tacgtgctcg gcgcgaacaa
     2581 cgcgttcagc tacctgtcct gcccgatggc gaacatgacg aatcagctca acggcctcga
     2641 atcaggctgg attcgctttc gcgtcaccaa caatcagagc tcttgcagca tgcgctccat
     2701 gactgcgtgg ggctccgtgg tgtcaggcag cgcggtcacc ctgacgacct tgaatagcga
     2761 tcactcactc aacatcggcg cgaacgtcac gtcgagcaac tcgaacgggt tctttacgct
     2821 cggctgcacg ctcagcggcg ggagccggct gtactcgtat cgctacgaag aggcgccctg
     2881 aggcatcggc tcgccggctt ccgatagacg agcgaatgcg cgcgacgact cgagggagag
     2941 cttcgggtcg tcgcctttca cggcgactcg cgttgactcg acggggaggg ccttacttcg
     3001 gccggacgcg cttcaacatc cgattcagat ggctgcgctc gatgccggac aggcgcgcgg
     3061 cctccgactg gttgccctgc gtgtggcgca ggaggtgctc gaggtacacg acgacgaagc
     3121 gttcgagcag gtcctccttc aagtcggcgt agcgccgctc gatgtcgacg aaatgctgta
     3181 aggcgagttg caaatcaggt gcgcgcgcgt ccggagaacg cgaaggctgc tcgaatccgc
     3241 cgagggcaat gtaggagcga acggcgttct tcaactcgcg aacgttgccg ggccaggtgc
     3301 gctgcagcca gcgcgtcacg acctcggggg gcacctcgct cgaggccatt tcttccgcga
     3361 agtgacgcac gaggacggga atgtcttccg ggcgctctcg gagcggcggg acgtgcaccg
     3421 tcgcaaccac gagccggtag aataggtctt ccctgaacac gccctcttgt acgagttggt
     3481 cgagtgcgcg atgcgtcgcc gagatcacgc ggacgtccac gggtcgttcg cggttctcgc
     3541 ccaggcgagt caccgtcccg acctcgagcg cgcgcaggag catcggctgc acatcgaggg
     3601 gtagctcggc cacttcgtcg aggaacagcg tgccgccact cgcttgctcg aatacgccgg
     3661 cgtgcgcgtc gcttgcgccc gtgaacgccc cccgcgcatg tccgaacagt tccgagcgga
     3721 cgagcgctcg atccaaggcg ccgcagttga cc
//
LOCUS       DASTKS010001184         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1082114, whole genome shotgun sequence.
ACCESSION   DASTKS010001184 DASTKS010000000
VERSION     DASTKS010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082114"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..2288
                     /locus_tag="VFQ35_25180"
     CDS             453..2288
                     /locus_tag="VFQ35_25180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9934031.1"
                     /translation="MAHLRPSFRPLFAIAFAALGLASCHEGAPSRGSGKAYPREQTLY
                     VGGRQWGEPSSFNPLAGNPDWPVISGVKLLYETLFLYDSLSGKLAPLLAESYARRDGA
                     IEVVLHEKARWNDGRPVTAWDVKYSFELGKRYKDTPVAQVWPYLKEIQLPEVSPGTAE
                     GEGHPRKLLFILNPERENPLVLLDGFQEFRIVPRHVIQPLLASVNDDMSRFLKIKFHD
                     AAQAVCSGPYKIDSIQSEKIGVVRNDDYWGNEVLYGGKKPAPKYIVHPIYKSNDHFSV
                     ALQQGKLDVSSTYIPRIWLKQRKGVHTWFSKEPFYLPQAIPSLFINHGHKPLGDVHLR
                     RAMGFAINYKDVRELAVSGYSPPLKPGLILPFGVESKYYSEEDAKKYGTSYDPERAKA
                     ELREGGYTAVWGAKGELVETRDARGNKVPTLYVKSPTGWTDWESIVSIVVQSMRAVGI
                     DVRERFVDASLFWQSLYVGNFDLIMWTPYAQPTPSKPWSRLDYVMTSADFAPEGQKMF
                     KNMGRFNDPKSPAYVPRVDELLRRIPNLTDERELTEAYRELNALFMQLQPSLPLVYRA
                     DQQYQFSTRVWENWATAENPYAPPQVPGDRMGTPILWHLRPKAAN"
     gene            2288..2908
                     /locus_tag="VFQ35_25185"
     CDS             2288..2908
                     /locus_tag="VFQ35_25185"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET9934032.1"
                     /translation="MSMIEHARRPVRLLVWGALFVAASVTVGCGSEDPKKAPTEVGGP
                     FGKCNAMIAKYQVTSADHVAECSELPDADPPYGGPHYPVWAAFQAYDFPIAHGYLIHS
                     MEHGAVVFYYNCPEGCSDEVAQAKALIEQEPKDRLCEGTGVERRAVLVPDPTLDVRWA
                     ASAWGYTLRAECFDADVFGQFYTDHYAMAPENICASGTVFTSPPCQ"
     gene            3027..>3761
                     /locus_tag="VFQ35_25190"
     CDS             3027..>3761
                     /locus_tag="VFQ35_25190"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9934033.1"
                     /translation="MSALSLLSADIEALIERARSSVVAVEHGRGHGSGVVVSQDGYVL
                     TNAHVVGTERSLRVRFADGEELAASLVGRDAPSDLAVIRVPASGLGALALHETRRVRV
                     GQIVLALGNPLRLEGSASLGIVSALDRSLPGPRGRALEGLIQTDAAINPGSSGGPLLD
                     ADGCVVGINTAMMAMASAIGFAIPAYTATWVTAMLIKHGAIRRPWIGVAASGVELTRR
                     TASDSGRTRGVRVHEVVADSPAAHAGV"
BASE COUNT          712 a         1211 c         1204 g          634 t
ORIGIN      
        1 catgatcgtg tcgatggggc tcgcgtcgcc gagccgcggc aagaagaagt tgatcgttac
       61 ggccaccaag aacgtgacga agtaccaacc agcgcggctc aaaacgtgcc gaagcgacgg
      121 gtggttcctc agcatgaagc atcctcatgc gagacgctca aggtgcactc tgccaaattt
      181 caggggtttt cgtaggcgat ggcccacagt gtgtcaaggc gacggtcagc gctcgctggg
      241 ctctcgagct ccgacgccga ccgagcaccg tacgaagcga gtgagcgcga acacgtagct
      301 ctccccgtcg acgtaaaatc gtgaacgacg ccagccgcac gcagacacac gactcgagag
      361 ggaacgtcgc cttgacacgg caagccccct cgcctacgaa aagtgccgaa aattgcggag
      421 ctttgccgaa gtttgctcgc aatccgtgct cgatggcgca tcttcgtccc tcgttcagac
      481 cgctcttcgc aatcgccttc gccgcgctcg gcctcgcgag ctgccacgag ggcgccccca
      541 gccgtgggtc ggggaaggcg tacccgcgcg agcaaacgct gtacgtgggc ggccggcaat
      601 ggggcgagcc gagctcgttc aatccgctcg cgggcaaccc ggattggccc gtcatctcgg
      661 gcgtgaagct gctctacgag acgctctttc tctacgattc cttgtccggg aagctcgcac
      721 cgctgctcgc ggagtcgtac gcgcgccgcg acggagcgat cgaagtcgtg ttgcacgaaa
      781 aggcgcgctg gaacgacggt cgccccgtga cggcgtggga cgtcaagtac agcttcgagc
      841 tcggcaagag gtacaaggac acgccggttg cgcaagtctg gccgtacctg aaggaaatcc
      901 agctgccgga agtgagcccc ggcacggccg aaggcgaagg gcacccgcgc aagctcttgt
      961 tcatcctgaa cccagagcga gagaacccgc tcgtcttgct cgacgggttt caggagtttc
     1021 gcatcgttcc gcgccacgtg atccagccgc tgctcgcgag cgtcaatgac gacatgagcc
     1081 gcttcctgaa gatcaagttt cacgatgcgg cgcaggcggt gtgctccggg ccttataaaa
     1141 tcgactcgat tcagagcgag aagatcggcg tggtgcgcaa cgacgactac tggggcaacg
     1201 aggtgctgta cggcgggaag aagcccgcgc cgaagtacat cgtgcaccca atctacaaga
     1261 gcaacgatca cttcagcgtc gcgctccagc aaggcaagct cgacgtatct tcgacgtaca
     1321 ttccgcgcat ttggctcaag caacgcaaag gcgtccacac ctggttttcg aaagagccgt
     1381 tctacctgcc ccaggcgatt ccttcgctct tcatcaatca cgggcacaag ccgctcggcg
     1441 acgtgcacct gcggcgcgcg atgggctttg ccatcaacta caaagacgtg cgagagctcg
     1501 cggtctcggg gtacagccct cccctcaaac ccggcttgat tctgcctttt ggcgtcgaaa
     1561 gtaagtacta ctccgaggag gacgcgaaga agtacggcac gtcctacgat cccgagcgcg
     1621 ccaaggccga gctgcgcgaa ggcggctaca ccgcggtctg gggagcgaag ggcgagctcg
     1681 tcgagacacg cgacgctcga gggaacaagg tcccgactct ttacgtgaag tcgccgaccg
     1741 gctggaccga ttgggagtcc atcgtgagca tcgtcgtgca gagcatgcgc gcggtcggca
     1801 tcgacgtgcg cgagcgcttc gtcgacgcga gcttgttttg gcagtcgctc tacgtgggca
     1861 acttcgacct catcatgtgg acgccgtacg ctcagcccac cccttccaag ccgtggtctc
     1921 ggctcgacta cgtgatgacg tccgctgact tcgctcccga ggggcagaag atgttcaaga
     1981 acatggggcg cttcaatgat ccgaaatccc ctgcttacgt gccgcgcgtc gacgagctct
     2041 tgcggcgcat cccgaacctc accgacgaac gcgagctcac ggaggcgtac cgcgaactca
     2101 acgcgctctt catgcagctg cagccgtccc tgcccctcgt gtaccgcgcc gaccaacaat
     2161 atcaattttc cacgcgcgtt tgggaaaact gggcgacggc cgagaaccct tacgctccac
     2221 cccaagtgcc gggggaccgc atgggaacgc ccatcctctg gcacttacga ccaaaggctg
     2281 caaactgatg tcgatgatcg agcacgcgag gcgcccggta agactcttgg tttggggggc
     2341 gctcttcgtg gcggccagcg tcaccgtcgg atgcggcagc gaagacccga agaaggcgcc
     2401 gaccgaggtg ggcggcccgt tcggcaagtg caacgcaatg atcgcgaagt accaagtcac
     2461 ttcggcggat cacgtcgcgg agtgctccga gctgcccgac gcggatcctc cctacggcgg
     2521 accgcattac ccggtttggg cggcgtttca ggcttacgac ttccccatcg cgcacggcta
     2581 cctcatccac tccatggagc acggcgcggt cgtcttttat tacaattgcc ccgaggggtg
     2641 ctcggacgaa gtcgcccagg cgaaagcgct catcgagcag gagccaaagg accggctctg
     2701 tgaaggcacg ggcgtggagc ggcgcgcggt gctcgtgccg gatccgacgc tcgacgtgcg
     2761 ttgggccgcg agtgcctggg gctatacgct gcgcgccgag tgcttcgacg ccgacgtgtt
     2821 cggtcaattc tacaccgacc actacgcgat ggccccggag aacatctgcg cctcgggcac
     2881 cgtgttcact agccctccct gccagtaagt agtcgactcg ccgcttcgag ggcgaaaccg
     2941 aaaccggtgt gcctgcccga acggctcagg gctgcctcgc cggtcgagca gtgtcgtcca
     3001 atcgcgacgg agcgcacgct atggacatga gcgcgctctc cctactctcc gcggacatcg
     3061 aagcactgat cgaacgagcg cgctcgagcg tcgtcgcggt cgagcatgga cgcggacacg
     3121 gcagcggggt ggtggtgagt caagacgggt acgtcctgac caacgcgcac gtcgtcggaa
     3181 ccgaacgtag cctgcgggtg cggttcgcgg atggcgaaga gctcgcggct tcactcgtcg
     3241 gccgagatgc gccgagcgat ctcgctgtga tacgagtccc cgcgagcggc ctcggcgccc
     3301 tggctttgca cgagactcgg cgcgttcgcg taggccagat cgtgctcgcg ctcggcaacc
     3361 cgctgcgcct cgaaggctcg gcgagccttg ggatcgtgag cgcgctcgac cggagcctcc
     3421 cgggtccgcg cggacgcgcg ctcgaagggc tgattcaaac cgatgctgcc atcaatcccg
     3481 gttcgtcggg agggccgttg ctcgacgcgg atggctgcgt ggtcgggatc aacaccgcga
     3541 tgatggcgat ggcgagcgcg attggcttcg cgatcccggc gtacacggca acgtgggtaa
     3601 cggcgatgct catcaagcac ggagccattc gacgcccctg gatcggcgtc gccgcgagcg
     3661 gtgtcgagct cacacggcgc accgcgagcg acagcggacg cacgcgcggc gtgcgcgtcc
     3721 acgaggtcgt ggcggactcg ccagcggcgc acgccggagt g
//
LOCUS       DASTKS010001185         5965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1306927, whole genome shotgun sequence.
ACCESSION   DASTKS010001185 DASTKS010000000
VERSION     DASTKS010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306927"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFQ35_25195"
     CDS             <1..940
                     /locus_tag="VFQ35_25195"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HET9934034.1"
                     /translation="RPSNRPQFESLELDMDFMGDWDPQAASATRRAPGPAKPAIDPRK
                     DVAPARAPAQHRQPEQPTAAERRAQAEQATAELEKAAAREFSQPEQRRPGAPLKPPPK
                     PGRAIQESRPDENPLPPAAAAPSRTDVDPGALKPAAIASRTDADVAAPKPAAIASRTE
                     ADVAAPSPAIASRSEAEVAAPKPTAPKAVAAEAQEPKASPAEPAPTDAEREAGEGRAG
                     KPRQERAEPRAKAAKPSAKGPAPTLSDQRLKEVHERLLEAHKSTGQATSPSYDGLAKS
                     LRAVEAKLRAQHGSNRRVDFEVVVKDGKPVVKPIVR"
     gene            complement(961..1119)
                     /locus_tag="VFQ35_25200"
     CDS             complement(961..1119)
                     /locus_tag="VFQ35_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934035.1"
                     /translation="MVDSDPELSELCARMREAGRTACSILFCDGDVYVEPAAPSRPLA
                     SPARRAVS"
     gene            complement(1594..3324)
                     /locus_tag="VFQ35_25205"
     CDS             complement(1594..3324)
                     /locus_tag="VFQ35_25205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934036.1"
                     /translation="MQCEACGHSNSEGARFCANCGALLPPEQKGTDPLIGQLVGGRFR
                     VTGVLGEGGMGVVYVGEQQMGSTVRKVAIKTLHQHLSKDPSVRARFHRECGTVAQLEH
                     PNTIKFYDFGETSDGTLYIAMEFVAGRSLADVLQREGPLDPERVVRIMRQVCGALDEA
                     HGQGIIHRDLKPENVILTNRAGETDVVKVLDFGIAARSESADAQREQKLTQQGMVLGT
                     PPYMSPEQFTGKALDPRSDVYSLGVMAYELLTGRLPFDADTPWQWATQHMTAPPIAFE
                     VAAPTKNIAPPMRQAIMKALSKDRDQRQGSAREFFTEFSDGGRVTVDAPAPVAGHQST
                     GTAAMDVMPPAFNPPVPQVHAPAGPMGTPPGVAAVPPAPTRPGRSGGGKGLVIGLGAV
                     GAVLLIAIAVVAARTIKPEDDKLPPPADNTAPSSAAPPANTGATTIAPLETAPPAPSV
                     TAPPDSPTTPASPTHSTSPNTPATPKTNTGSTTTTPKPPTGGNCDACASLASSENIGG
                     AVAAMNSCTDAAKKASCGSAIRRAAPSAVKHAALNGNCSQAKGIIAAAQAAGVPAAAL
                     NGALAGTSCK"
     gene            complement(3390..4097)
                     /locus_tag="VFQ35_25210"
     CDS             complement(3390..4097)
                     /locus_tag="VFQ35_25210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934037.1"
                     /translation="MYGRRIHRLTSAPALLLVVLAEAIACSASRPPTRSELSPGVVFT
                     HHIAEGTGDGFGPEPPRPSATPALQGEAGAPNEPAPAAPPPSASPSSKPLEGPPDPDP
                     LRLSDQWEYELSFDAGKVAVVAARARHFDKPVVSARRIGRFAIELWIGRELVERIRFD
                     FPGLGLEARPAEQGTRRPLFPPANFAQGVHSRQTVLVPAAPRARRALLVDRATGESQE
                     LPWPPDHPWNQPAQTPP"
     gene            complement(4301..5173)
                     /locus_tag="VFQ35_25215"
     CDS             complement(4301..5173)
                     /locus_tag="VFQ35_25215"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9934038.1"
                     /translation="MSEPTTLGSLLDQRGERGALERGGVFVDGQRTESATASLEPGAR
                     VEVYAAREEGASVGILAQERGLVFAYKPVGLASEPERRGNASVVTLVAEMLKLDVARV
                     HALTRLDVGVSGVVLLGLDSAARQRVHSLREAGAFERRYVALAHSTPNPANGTWNEAL
                     GKAPGGARRCVSSHGEPATTRYSAVASTLSGASILAFRPLTGRTHQLRVHASFHGAPL
                     LGDSTYGGSKRLVLAGGRVIGLPRIYLHAAWIALADQSRVETPLPADFGSTWAVLGGK
                     DSDLDRALVEPLGS"
     gene            complement(5197..>5965)
                     /locus_tag="VFQ35_25220"
     CDS             complement(5197..>5965)
                     /locus_tag="VFQ35_25220"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET9934039.1"
                     /translation="WLLDRALMLLSSVDSRAFEARLTGARHSREQRVGAALAATSRAD
                     GALSKLVIAAGRRSAVLRARLYVVRCRALFMLRTVVLDMDRRLGRLLGTAPEAAFFLR
                     LGELLESTWRPDPKLSEVTRERHASWRAACDRQAPPPALGRAALDPEGEPLRGFGVGD
                     ATLDGTVTVAHEFERALELEPGGVLVVRSLEPGWGPILPLAGAIVTDAGGATSEGVVA
                     AAALGVPLIIGTRRATSALTSGQRVTVDTRAGTVTTT"
BASE COUNT         1021 a         2050 c         2002 g          892 t
ORIGIN      
        1 gcgcccaagc aaccgaccgc agttcgaatc actcgaactc gacatggact tcatgggcga
       61 ctgggatccg caggcggcga gcgcaactcg tcgcgcgccc ggacctgcga aaccagcgat
      121 cgacccccgc aaagacgtcg ccccagcgcg cgcgcccgcc caacatcgcc aacctgagca
      181 acccacggcg gctgaacgtc gagcccaggc cgaacaggcg acagcggagc tcgaaaaggc
      241 cgccgcccga gagttttcac agcccgagca gcgtcgtccg ggggcaccgc tcaagccgcc
      301 gcccaaaccc gggagagcga ttcaagaatc gcggcccgat gagaatccct tgcctcccgc
      361 cgccgcggct ccgagccgaa ccgacgtaga cccgggcgcg ctgaagcccg ccgccatcgc
      421 gagccgcacc gacgccgatg tcgcggctcc gaaacccgcc gccatcgcga gccgaaccga
      481 ggccgatgta gcggctccaa gtcccgccat cgcgagccgc agcgaggccg aagtcgcggc
      541 tccgaagccg acggctccga aagccgtcgc cgccgaagcg caagagccca aagcctctcc
      601 cgcagagccg gcgcccaccg atgccgagcg cgaagcaggc gaagggcgcg ccggaaagcc
      661 gcgacaagag cgcgccgagc ctcgcgcgaa agcggcgaaa ccatccgcca aaggccccgc
      721 acctacattg agcgaccagc gcttgaagga agtgcacgag cgcctcttgg aggctcacaa
      781 gagcactggc caagctacgt cgccgagcta cgacgggctc gccaaatcgc tgcgcgccgt
      841 cgaagcgaag ctccgagccc agcacggcag caaccgccgc gtcgacttcg aagtcgtggt
      901 caaagacgga aagccggtcg tcaaaccgat cgtccgctga gccgaagggc ccgcgtacgt
      961 tcagctgacc gcgcggcgtg ccggacttgc gagcggtcgc gacggagctg cgggctcgac
     1021 gtacacgtcg ccgtcacaga aaagaatcga gcaggcggtg cggcccgcct cgcgcatgcg
     1081 cgcacagagc tcactcagct ctgggtcgga gtcgaccacg tctccctcga cgggctgccg
     1141 ggtctcatga tcataacgac agttatcaat cgcgacccac aggccacgaa atttggcgga
     1201 gcggcaaagc tccggccacg tcatcctagt cgtcatcgct gaatgtacct tctcgtgagt
     1261 atccccctca cggggcacag cgtcgggctt tcctttatag cttgcgtaac gagcgccacg
     1321 ggcaatcgac ctgggggcat atcaatacaa gttcccatgt ccgtcaagct atcaccagga
     1381 agcgtcgcgt caccgaatta gcataatgat ctcaagcgga taggaggcgt cgaacctcgc
     1441 cggatccgca cctctgaaca cgcgcatagc aggaatcagc gccgttcggc cgactgaaaa
     1501 gcgcctcgct gggccactat tgacctcaag cgcgcctcgc aattgccgca agcgtgacgc
     1561 gaacgcgttg cacgcgacgc gctccggcag cgcctacttg cagctcgtgc cggcgagcgc
     1621 tccattgaga gcggcagcgg ggacaccagc ggcttgagcg gcggcgatga tgcctttcgc
     1681 ctgagaacaa ttgccgttca gcgctgcgtg tttcaccgcg gagggcgcgg cgcggcggat
     1741 tgcgctgcca cagctcgcct tcttcgcggc atcggtgcag ctgttcatcg cggcgaccgc
     1801 gccgccgatg ttttcgctgc tcgccaggga cgcacatgcg tcgcaattgc caccggtcgg
     1861 cggcttcggt gtcgtggtgg tggagcccgt gttcgtcttc ggagttgcgg gcgtgttcgg
     1921 ggacgttgag tgcgtcgggg aagcgggagt cgtcggcgaa tcgggcggag ccgtgaccga
     1981 tggcgccggc ggcgccgttt ccagcggagc gatcgtcgtc gcgccggtat ttgccggcgg
     2041 agccgccgaa cttggcgccg tgttgtcggc gggcggtggc aacttgtcgt cctcgggttt
     2101 gatggtgcgc gccgcgacga ctgcgatggc gatgagcagc acggcgccga ccgcgccgag
     2161 cccaatcacc aagcctttcc cgccaccgct ccgaccggga cgagtcggcg cagggggaac
     2221 tgcggccacg cctggaggcg ttcccattgg accggcgggc gcatgcacct gcggcaccgg
     2281 cgggttgaat gcgggcggca tgacgtccat cgccgcggtg ccggtgctct gatggccggc
     2341 gacgggcgcg ggagcgtcca ccgtgacgcg acctccgtca gaaaactccg taaagaattc
     2401 gcgagcactg ccctgacgct gatcgcgatc cttcgagagg gccttcatga tcgcttggcg
     2461 catcgggggc gcgatgttct tcgtcggcgc ggcgacctcg aaggcgatcg gaggcgcggt
     2521 catgtgctgc gtcgcccact gccagggcgt atccgcgtcg aacggaagcc gcccggtgag
     2581 cagctcgtaa gccatgacgc cgagcgaata cacgtcgctg cgtggatcga gcgcttttcc
     2641 agtgaactgc tcggggctca tgtagggagg tgtgccgagc accatccctt gttgcgtcag
     2701 cttctgctct cgctgcgcat ccgcggactc gctgcgcgcc gcgatgccga agtcgagcac
     2761 tttgacgacg tcggtctcac ccgcacggtt ggtgagaatt acgttttcgg gcttgagatc
     2821 gcggtgaatg atgccttggc cgtgcgcttc gtcgagcgcg ccgcacacct gtcgcatgat
     2881 ccgaacgacg cgctccggat cgagcggccc ttcgcgctga agaacgtcgg cgagcgagcg
     2941 ccccgcaacg aactccatcg cgatgtacag cgtgccatcg gaggtctcgc cgaaatcgta
     3001 gaacttgatg gtgttcgggt gctctagctg cgcgacggtc ccgcactcgc ggtgaaaacg
     3061 cgcgcgcacc gacggatcct tcgagagatg ctgatggagc gttttgattg cgactttgcg
     3121 gaccgtcgag cccatctgct gctcgccgac atagacgacg cccatgccgc cctcgccgag
     3181 cacacccgta acgcggaacc tgccgcccac gagctgacca atcagaggat cggtgccctt
     3241 ctgctccggc gggaggagcg cgccacaatt ggcgcaaaag cgcgctccct ccgaattgct
     3301 atgaccgcaa gcttcgcact gcatcgcccc gcaagcgtag gccagacacg gcgcgtggcg
     3361 ctacggaatc ttccgtgtgg acgagccacc tacggtgggg tctgcgcagg ctggttccac
     3421 gggtggtccg gcggccacgg cagctcttgg ctctcccccg tcgcccgatc gaccagcagc
     3481 gcccgccgag cgcgcggtgc agctggaacc aagaccgttt ggcgcgagtg aacgccttgg
     3541 gcgaagttgg ccggcggaaa caagggacgg cgggttccct gctcagctgg ccgcgcttcg
     3601 aggcccaagc cggggaagtc gaagcgaatg cgctcgacga gttcgcgccc gatccaaagc
     3661 tcgatggcga agcgcccgat tcggcgcgcg ctcaccaccg gtttgtcgaa atgacgggcg
     3721 cgtgctgcga cgacggcgac cttcccggcg tcgaacgaga gctcgtactc ccactggtcc
     3781 gagagtcgca gcggatccgg atccggcgga ccctcgaggg gcttcgagct tgggctcgcg
     3841 ctgggcggtg gagcggccgg tgcaggctcg ttgggagcgc ctgcttcgcc ttgcaaagcc
     3901 ggggtggcgg agggcctcgg cggttccggc ccgaatccgt cgcctgtccc ctccgcaata
     3961 tgatgagtga agaccacgcc gggcgagagc tcgctacgcg tgggcggacg gctcgccgag
     4021 caggcgatcg cttccgccag gacgacgagc aaaagtgcgg gggccgaggt aaggcgatga
     4081 atgcgccgac catacatgag atggtcgcgc cgtctttcga attttgctcg gttggacgtg
     4141 acgcacagga ttcgcccgag cgtcggggcc agacgcaccc cctccggcca tcggcgccta
     4201 gcgggagaat cctctttctg gagtaagcca aagacaggcg ggccgttgcg cgcgagcgct
     4261 gtgtcgatgg cacccacgtt acgcgcttct tcgaactcgc tcaggagcca agcggttcga
     4321 cgagcgcgcg atcgaggtcg ctgtctttcc cgcccagaac cgcccaggtc gagccgaagt
     4381 cggccggaag cggcgtttcc acccgcgatt ggtcagccaa agcaatccaa gcggcgtgca
     4441 aatagattcg cggtaagccg atgacgcgac cgcccgccag caccaggcgc ttcgacccgc
     4501 cgtaggtcga gtccccgagt agcggcgctc cgtgaaatga agcgtgcact cgcagctggt
     4561 gcgtgcgccc ggtgagggga cgaaacgcga gaatggacgc gccgctcagg gtcgaggcga
     4621 ccgcgctgta ccgagtggtg gccggctctc cgtgggaact gacgcagcgc cgcgcaccac
     4681 cgggcgcctt gccgagcgcc tcgttccacg ttccgtttgc tgggtttggg gtcgagtggg
     4741 cgagcgcgac gtaacggcgc tcgaatgctc cggcttcgcg cagcgagtgc acgcgctgtc
     4801 gcgccgcgga gtcgaggcca aggagcacga ccccgctcac gccgacgtcg agccgcgtga
     4861 gcgcgtgcac gcgcgcaacg tcgagcttca acatttcggc gacgagcgtg acgacgctcg
     4921 cgttgccacg tcgttccggc tcgctcgcga gcccgaccgg cttgtaggcg aagacgagcc
     4981 ctcgctcttg cgcgaggatg cccaccgaag cgccttcttc gcgcgccgcg tacacctcga
     5041 cgcgcgcgcc cggctcgagc gacgccgtcg cgctctcggt tcgttgcccg tcgacgaaca
     5101 cgccaccccg ctcgagcgcc ccccgctcgc ctcgctggtc gagcagcgaa ccgagcgtcg
     5161 tgggctcgct cactacccag caaatccgcg ggcggctcac gtggtcgtca ccgtgcccgc
     5221 gcgcgtgtcg accgtgacgc gctggccgct cgtgagcgcg ctggtcgcgc gccgagtgcc
     5281 gatgatgaga gggacgccga gtgcggcggc ggcgacgacc ccttcgctcg tggcgccccc
     5341 cgcgtcggtc acgatcgcgc ccgcgagcgg cagaatcggg ccccaaccgg gctccagcga
     5401 gcgcacgacg agcacgccgc ccggttcgag ctcgagcgcg cgctcgaatt cgtgagcgac
     5461 cgttacggtt ccgtcgagcg tagcatcgcc gacgccgaaa ccacgcagcg gctccccttc
     5521 gggatcgagc gcagcgcgcc cgagcgccgg cggaggcgct tgtcggtcgc acgccgccct
     5581 ccacgatgcg tgccgttcgc gggtcacctc cgagagcttc gggtcgggcc gccacgtgct
     5641 ctccaggagt tcacccaggc ggaggaaaaa cgcggcttcc ggcgccgtgc cgagcaaacg
     5701 cccgagcctg cggtccatat cgaggacgac ggtccgcaac atgaagagag cgcgacagcg
     5761 tacgacgtag agccgcgcgc gcagcacggc cgagcgtcgt cccgctgcga tcaccagctt
     5821 cgagagcgcg ccgtcggccc gcgacgtcgc tgcgagcgcc gctccgacgc gctgctcgcg
     5881 agagtgccgc gcccccgtga gccgcgcctc gaaagcgcgt gaatcgacgc tcgagagcag
     5941 catgagcgct cgatcgagca gccac
//
LOCUS       DASTKS010001186         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1082250, whole genome shotgun sequence.
ACCESSION   DASTKS010001186 DASTKS010000000
VERSION     DASTKS010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082250"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            713..1408
                     /locus_tag="VFQ35_25225"
     CDS             713..1408
                     /locus_tag="VFQ35_25225"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9934040.1"
                     /translation="MPPTPPHERRGFPRGYVAALASVLVNERHAGDFVVRDLSAGGAL
                     LVNGPLLPTGVECTVRLRVPGLGPLRVTAYTVHSAVVGNDRYAMGIRFTRLSPAVAEG
                     LRELVDQELDRAAQPSVLVADDDLSRLSDLAEDLADLGERPLLALTPLEVIHWLCDPE
                     TTVELALLSEELHQKSGSGVFDFVRGEFPHVRAVLLKDTLAREELRELLEWAAGPARS
                     ARQLKATQGYNTV"
     gene            1622..2563
                     /locus_tag="VFQ35_25230"
     CDS             1622..2563
                     /locus_tag="VFQ35_25230"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET9934041.1"
                     /translation="MTLRQPVGLVAVVAAASALLSTTRARATYSITAADTKTRELGGA
                     GASCVPYDVSKIYAGVPNRGAVNAQAEFDASALDYAAQRLSEGFDAHAVLAQVTSSAV
                     FPRAPYMQWGIVDVNGNLARATGTNTMPFAGDLEGRTPDRRFAFTTQGNILTSARVLE
                     RASAAFLSGSDAACDLAGRLMLALEAAGSMGEGDNRCTSTGIPAKSAYLEVTGVNGSV
                     FRVSTPDVSPNDPTPIVRKAFDEYRAAHPCPTQPTSETPPSDETPSTSSAGCACSLPR
                     AHGAPGAHLAFAALAALATFAVLLAERRTRRRPRVRN"
     gene            complement(2675..3373)
                     /locus_tag="VFQ35_25235"
     CDS             complement(2675..3373)
                     /locus_tag="VFQ35_25235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934042.1"
                     /translation="MLTRNEIERAVELQTKGYLLLQWLEKALEEGFIAPEAAHVYASM
                     EDSAFAWIERHYLNLPVAARPAREDLVPFSRLFSTYLTNTFDLEADPGERLYSPDAHC
                     FCPCCSWMVRVPHLKPKKLGAADKKAAESLKRAFLRKLAVDATVRSSERLLDELLGGK
                     ELREEVGLCTYAHDLLQRLQGVAVGPATLALWRSFAWTAQGSPKHGFKLSADAVMQAQ
                     DAILRRIRQSAENE"
     gene            4096..4674
                     /locus_tag="VFQ35_25240"
     CDS             4096..4674
                     /locus_tag="VFQ35_25240"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9934043.1"
                     /translation="MALWGQKRKKSSRPAKPRAQEASRRAPSPPRGCVLRVGRATTAR
                     VYCRARPRAHSPRVLPQPPRRADALLRNGRLVLSNNWAERAIKPVATGRKAWLFCGSD
                     DHAKSAATLFSLVGSARLHGLDPEEYLRCLIRLVPLWPPERMLELSPLLLKRTRERLD
                     ADALSAEFGSIRIPAEPLDTTCPSQNEAPTAS"
     gene            complement(4671..4841)
                     /locus_tag="VFQ35_25245"
     CDS             complement(4671..4841)
                     /locus_tag="VFQ35_25245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934044.1"
                     /translation="MLFEGDLQLRGTLTDPPDEPIFVAGVVSGAEFNVDVLYQLHAAE
                     RVASEWGAWRAS"
     gene            complement(4928..>5985)
                     /locus_tag="VFQ35_25250"
     CDS             complement(4928..>5985)
                     /locus_tag="VFQ35_25250"
                     /inference="COORDINATES: protein motif:HMM:NF013729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9934045.1"
                     /translation="NVQHANLQGVNLRDANLSSANLGGAILDDANLTITNLSGAGLIG
                     ATLRGANLMSALLTRASLSDANLSDASLSGANLRGASLRGANFTSADLIDADLTGADL
                     SGADLSGATLEGTTLVGVNLTQSQNLAAVRHFGPSHLGEVSLMLARGQLPEAFLRGVG
                     FSDWQILIAKLYNPGLRAEARTEILYEIVRLQQGMPIVFHSVFISYSTQDQEFCRKLH
                     ADLQSAGVRCWFAPHDIKAGQKLHEQIDRAITLHDRLLLVLSDASMSSQWVKTEIANA
                     RAKERTQNRAVLFPVRLVSFEAVKNWKLFDADAGNDSAREIREYYIPDFSRWRDEADY
                     QPALKTLVNALRDATKV"
BASE COUNT         1057 a         2064 c         1772 g         1092 t
ORIGIN      
        1 gccgttccca gccttgcgcc gatcgtctcc cggcgcctct cccccgctcg ctcccgacaa
       61 cctcgcaaac ctcgctggcg acctcgcact ccccttcgct tcgcgacgcc tcgcaaccct
      121 cgctggcgcc tcgcactccc cttcgcttcg cgacgccccg caaccttcgc tggcgcctcg
      181 cactcccctt cgcttcgcga cgcctcgcaa ccctcgctgg cgcctcgcgc tccccactcg
      241 cttcgcgaca cgctcccacc gcttctcgcc gcctcgcacg cctcccctcg attgcgccct
      301 cgttcggtcg acatcggaag cccagagcgc gtcatcccgc ccgagccgga gccggagccg
      361 caattctgta ccaatcgcgc cggtcggcgc actcgaacgc gcaatgtggc cggggtcgcc
      421 gccggaacgt gcgcgacata taccgaattc tgaccaggag agcgccaaga caaggccttc
      481 ggacttcata ggcccgaacc cggccgctcg tgaccggtgc gcccactgcg cggcggctca
      541 tgccttgtca gcaccttcta gtttgtcggg attttgacag cggtgtcgcc aagtacccga
      601 aactgcgcgg gaatcgccgg ggcgaaattt gcctgccccc cctcaacttg gcgtccgcgc
      661 ggaccgtttc taccaacgtg cccgcctggt gcggggccac gtgccaattc gcatgcctcc
      721 tacacctccc cacgagcgac gtggcttccc ccgcggatac gtcgcggcgc tcgcgagcgt
      781 tttagtcaac gagcgacacg cgggggattt cgtcgttcgc gacctctcgg cgggcggtgc
      841 gcttctcgtc aacggacctt tgctcccgac cggcgtcgag tgcaccgttc gactgcgcgt
      901 ccctggcctc ggcccgctgc gcgtcaccgc gtacaccgtg cattcggcgg tggttggcaa
      961 cgaccgctat gcaatgggga tccgcttcac gcgcctctca ccggcagtcg ccgaggggct
     1021 gcgcgaacta gtcgaccaag aactcgaccg cgctgcacag ccttcggtgc tcgtggcaga
     1081 cgacgacctc tcgcgcctgt ccgacttggc agaagacctc gcggacctgg gcgagcgccc
     1141 gctgctcgcg ctcactccgc tcgaagtcat ccactggctc tgcgatcccg aaaccaccgt
     1201 tgaacttgcc ctgctgagcg aggagctcca ccaaaagtcc ggcagcggcg tcttcgactt
     1261 cgtgcgcggt gagttccccc acgtgcgcgc agtcctcctc aaagacaccc tcgcccgcga
     1321 ggaactgcgc gagctcctcg aatgggcggc gggccccgcg cgctccgcgc gtcagctcaa
     1381 ggccacgcag ggctataata cggtctaggg ggtgtcccaa gttccccgcc ctgctgcgag
     1441 ccaagatcct gcggctgcga agcacggcct cgtcgctccg ctcctcgaaa ggcttcagcc
     1501 ttgctccggt gctcactcct cgggcgcgct tctcgctcgc gtttcttggc tctcgcgacc
     1561 ggcaggaact tgggacaccc cctagttcgc cgagcgcgaa accggctacg ttgaacgccg
     1621 tttgacgctt cgccagccag tcggactcgt agccgttgtc gcagccgctt ctgcgctgct
     1681 ctcgacgacg cgcgcacgcg ccacgtactc gattaccgcc gccgacacga agacacgcga
     1741 gctgggcggc gcgggcgctt cgtgcgtccc ctacgatgtc agcaagatct acgctggcgt
     1801 ccccaatcgc ggagccgtca acgcgcaagc cgagttcgat gccagcgccc tcgactacgc
     1861 ggcgcaacgg ctcagcgaag gcttcgacgc gcacgctgtg ctcgcccaag tcacgtcctc
     1921 tgcggtcttt cctcgcgcgc cttacatgca gtggggcatc gtcgacgtga acggaaatct
     1981 cgcgcgagcc acgggcacca acaccatgcc cttcgctggt gacctcgaag gccgtacccc
     2041 agaccggcgg tttgcgttca ccacacaagg caacatcctc acctcggccc gcgtcctcga
     2101 acgcgccagc gcggcgttcc tttccggctc agacgccgcg tgcgacctcg ccggacgtct
     2161 catgctcgcg ctcgaagcag ccggctcgat gggcgaaggc gacaatcgct gtacatccac
     2221 cggcataccc gcgaagagcg cctacctcga agtcaccgga gtcaacggct ccgtctttcg
     2281 cgtcagcact cccgacgtct cgcccaacga cccaacccca atcgtccgca aagctttcga
     2341 cgagtatcgc gccgcgcacc cgtgcccaac gcagccaact agcgaaacgc cgccaagtga
     2401 cgaaaccccg agcacgagca gcgctgggtg cgcgtgctca ctcccccgag cacatggcgc
     2461 accgggggca cacctcgcat tcgcggcgct cgcggcgctc gcgacgttcg cggtgctcct
     2521 cgctgagcgc cggactcgtc ggagaccgcg agtgcgcaac tgagcaaagg gcagaccaca
     2581 cgggccgacc tcataggagt ccgattcgcg caaatccgaa ggcgcgtctc cgtcaactcc
     2641 atccggccct ggcccattcg acgttgaatg cgggtcattc gttctcggca ctctgcctaa
     2701 tacggcgaag gattgcgtct tgcgcttgca tcacggcgtc cgcagacaac ttgaaaccat
     2761 gcttcggtga accctgtgcc gtccaagcga agctgcgcca aagtgctagc gtcgcgggac
     2821 caacggcgac tccctgaagc cgctgcagca gatcgtgagc ataagtacaa agcccgactt
     2881 cctcccgaag ctccttccca ccgagcaatt cgtcgagaag tcgctccgat gagcgtacgg
     2941 tcgcatcaac ggccagtttc cgcagaaacg ctcgcttcaa gctctctgca gccttcttgt
     3001 ctgccgcgcc cagcttcttt ggtttgagat gtggcacccg aaccatccaa ctgcagcagg
     3061 gacagaagca gtgcgcgtcg ggcgagtaga gccgctcgcc cgggtcagcc tcgaggtcga
     3121 aggtgttcgt gaggtaggtc gaaaaaagcc tgctaaacgg taccaagtct tcgcgggcgg
     3181 gacgcgcggc aacaggaagg ttgagatagt gacgttcgat ccaggcgaac gccgaatctt
     3241 ccatgctggc atagacgtga gccgcctccg gcgcgatgaa gccctcctcg agcgcctttt
     3301 cgagccactg cagcaatagg tagcctttgg tttgaagctc gacagcacgt tcaatttcat
     3361 tgcgcgtcag cacgcctctc acataccgga gctcctgccc gcgatccagg aacgtcgact
     3421 cttgtcggcg gcttccccat gcgaaagagc cgcggcagcg cccccccgta atcacacacc
     3481 agactcaagc ctcgagaacg cggaggcgaa ggcccgcgac tgggtaagtc agactgcgcg
     3541 agatgaccct cttgctgcag cggacgggca actcgcccgc gactgactca tctgttcgcg
     3601 ggttgctaac gggcaccgct gcacgcggga tatcccgatt tgttcgagtt ccgccccgag
     3661 cgctcccaag gtgagttcgg gattcaacga acgcacaacc gcttcggtaa gaggacgatg
     3721 cgcgtagacg tgctcgagca ccccaaggtc aagccgcgct cgtagtactc ctcggcgaac
     3781 cgttgataag tggcgggctt gccatccacg cccgacaaca gaaattcgga gccgtcgggg
     3841 tctcgtccgt ccgggtagct aacgggacca ctccaccagc aattgtcgac ggcccgtcgc
     3901 caattacaga atgtagtatc ctggtatccg cttcaagccc ccgtgtagcg aatgtgatgt
     3961 agtcggccgc gctgacgcgc atggcgaaga gcgacgagtg cgtgagcgag tcgaagcgtg
     4021 gcgggcgagc ggaaagacgg cggccgaatt ttgttcggga caggactaca ggccgaagag
     4081 tcttcgatgg tgggcatggc actttggggg cagaagcgga agaagtcctc gcgcccagcg
     4141 aagccgagag ctcaagaagc ttcgagacgt gcaccttcgc ccccacgtgg gtgcgttctt
     4201 cgcgtgggtc gagcaacaac ggcccgcgtt tattgccgag cgcggcctcg tgcgcacagc
     4261 cctcgagtac tcccacaacc accgagacgc gctgatgcgc ttcttcgaaa tggtcgcctc
     4321 gtgctgagta acaactgggc ggagcgcgcc atcaaacccg tcgcgaccgg gcgcaaagcg
     4381 tggctcttct gcggcagtga cgaccacgcg aagagcgccg ccacgttgtt ctcgctcgtc
     4441 ggctccgccc gcttgcacgg actcgacccc gaggagtacc tgcgctgcct catccgactc
     4501 gttccgctct ggcctccgga gcgcatgctc gagctcagcc cgttgctatt gaagcgtact
     4561 cgcgaacgac tcgacgccga cgctctctcc gctgagttcg gctctatccg catcccggcg
     4621 gagccgctcg atacgacgtg tccgtcgcag aacgaagctc cgaccgccag ctagctcgct
     4681 cgccacgcac cccactctga tgcgacgcgc tcggctgcgt gaagctgata caagacgtcg
     4741 acgttgaact ctgcaccgct gaccaccccg gctacgaaga tcggctcgtc gggtgggtcc
     4801 gtgagcgtgc ctcggagttg caggtcccct tcgaacagca aggaacattg cgaaagtcaa
     4861 cggacgacac ggcttgagtt gtcgcgcgaa ccctcgccca catcctcacg acccgagggc
     4921 gatcttttta aactttcgtc gcatcgcgca gcgcgttcac gagagttttg agcgccggct
     4981 gatagtccgc ttcgtcgcgc cagcgagaga agtctggaat atagtactct cgaatttctc
     5041 ttgcgctgtc gttccccgca tctgcgtcga acagtttcca attttttact gcttcgaacg
     5101 agaccagtcg aacgggaaag agcacggcgc gattctgtgt ccgctccttg gcacgcgcgt
     5161 tcgcgatttc cgtcttgacc cattggctcg acatgctcgc atccgaaagt acgagcagca
     5221 ggcggtcgtg cagggtaatc gcgcgatcga tctgctcgtg aagcttttgc ccggctttga
     5281 tatcgtgcgg cgcgaaccag caacgcacgc ccgcgctctg cagatcggcg tgaagcttcc
     5341 ggcagaattc ttggtcttgg gtgctgtagc tgatgaaaac ggagtggaac acgattggca
     5401 ttccttgctg gaggcgcact atctcgtaga gaatttcggt acgcgcctcc gcacgcaagc
     5461 ccggattgta aagtttagcg atcaggattt gccaatcgga gaagccgacc ccgcgcaaga
     5521 atgcttcggg gagctgcccg cgggcgagca tgagtgatac ctctccgaga tgactcggcc
     5581 cgaaatggcg aacagcggcg aggttctgcg attgagttag gttgacgcca accagcgtcg
     5641 tcccttccag cgtagcgcct gagaggtccg ccccggagag gtcggcgccg gtgaggtctg
     5701 cgtcgatcag gtccgcgctg gtgaagttgg ccccgcggag gctcgcgccg cggaggttcg
     5761 cgccactgag gctcgcatca ctgaggttcg cgtcgctgag gcttgctcgt gtgaggagcg
     5821 cactcatcag gttggctccg cggagagtgg caccgatgag ccctgcgcca ctgaggttcg
     5881 tgatggtgag gttcgcgtcg tccaagatcg cgccgccgag gtttgcgctg ctgaggttcg
     5941 cgtcgcgcag gttcacgccc tgcaggtttg cgtgctgaac attcg
//
LOCUS       DASTKS010001187         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1307041, whole genome shotgun sequence.
ACCESSION   DASTKS010001187 DASTKS010000000
VERSION     DASTKS010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307041"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ35_25255"
     CDS             <1..205
                     /locus_tag="VFQ35_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934046.1"
                     /translation="TATGGALASGGSTATGGSTNEGGSPAAAEGGTSTAEGGSTSSAE
                     GGGTSNPGDVTGGTGGAGGTSAG"
     gene            249..848
                     /locus_tag="VFQ35_25260"
     CDS             249..848
                     /locus_tag="VFQ35_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934047.1"
                     /translation="MLASSFARALALSALAASLLGCSSSGSTSTALPHPTVVEVSPLE
                     FASEPACDAGPGSLKRYIATLVDVTPTSATDSKPLDFKLPSSALQVEANGSIHYEPIP
                     CRLSVSFGWVVAGHSYQTELLGYDRDDLELLEPGFPIAVDPASRAVVEPRWTAMCGAA
                     SEDDSSDGGSGNVPERAVRAESDLTRTVRYCGEWLESQQ"
     gene            923..1747
                     /locus_tag="VFQ35_25265"
     CDS             923..1747
                     /locus_tag="VFQ35_25265"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9934048.1"
                     /translation="MSRVESPLQGSLPYPGRVLRIVMIAIAALWLAFAIGLNWAGAPP
                     ELFWALCGNTDKILHGEVWRLFTAPWLHLPVASLGHVFSALLGLYFLGASLEQQWGSA
                     RFAKFLFFAALLAYVTQMLAELVLPRSLEIAPADGYWFGAIPVVEAIAIAWALSFRGR
                     EVRLFFVLPVSSTGLILFVVGASVASVIIKAQAPSGLVAPFGGMLAGWLFGSGSPSPL
                     RRLWLKLRLAQLDVEAKREAQARRNRAARSGLKVITGGRDDDSEDDSKRGGGRWLN"
     gene            1898..2452
                     /gene="pyrR"
                     /locus_tag="VFQ35_25270"
     CDS             1898..2452
                     /gene="pyrR"
                     /locus_tag="VFQ35_25270"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9934049.1"
                     /translation="MHVLLDAEGVERSLRRMAGEIAERTRGTQDLMLVGIRRGGVPLA
                     ERLVAHLRELEGDEVPRGSVDITFYRDDAATRLPNPRIGPSEIPGSLDGRHVILVDDV
                     LFTGRTVRAALDALLDYGRPRRVELLVLVERNGRELPIQADYIGKSVELEDDERVDVV
                     ELGGQLSAIVQAANAPSLPPQGMP"
     gene            2449..3393
                     /locus_tag="VFQ35_25275"
     CDS             2449..3393
                     /locus_tag="VFQ35_25275"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9934050.1"
                     /translation="MTEQRLHHLLGIEDLNVASAEQLLSTAEAFFEVSRRPIRKVPTL
                     RGKTIVNVFYEASTRTRTSFELAGKRLSADVVNISVSSSSTSKGETLRDTASTLDAMR
                     PDVLVLRHSASGAPHYIARYTRAAVVNAGDGMHQHPTQALLDAFTIRRAKGKDLRGLR
                     VAICGDILHSRVARSNALLLGLLGAEVRLAGPRTLLPQRADALGVQIFDRIEPALEGA
                     DVVMVLRIQRERLGAALLPSLREYARYFGLNERRLALARPNAIVMHPGPMNRGVEINE
                     DIADGARSVILDQVEAGVAVRMAVLYHCASEPGSEPLS"
     gene            3647..4003
                     /locus_tag="VFQ35_25280"
     CDS             3647..4003
                     /locus_tag="VFQ35_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934051.1"
                     /translation="MRSLFLRTCAVSLVLGIPGLACGPEEGASQPPLGDGGYTAEPLG
                     GSTARGGSTGSGGSKGLGGSKGSGGTKGLGGAPGAGGRGLPTNTRTNTSTGTNTRTST
                     STRTSTNTRTSTNTRT"
     gene            4181..4858
                     /locus_tag="VFQ35_25285"
     CDS             4181..4858
                     /locus_tag="VFQ35_25285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9934052.1"
                     /translation="MALEFDGVNDYASLGTAGFPFARLPQTISFWLNPGRGAAEQTIM
                     TLNKDAESGVLLGLSNGVPTARSAYSGDVFVQASSALPSGEWHHLAYLFDGTDAAPHH
                     TLYVDGKVVGTGTLLPDKRTPNTAYMGSDNHEERCYGGLLDEVRVWAVARSPELIRAE
                     LAGEALEQEPPELVLYYTFDEAAGARVVDRSGRQNHALLGDGLDDYMPRRVLSGVVRA
                     RAALGKP"
     gene            complement(4914..5690)
                     /locus_tag="VFQ35_25290"
     CDS             complement(4914..5690)
                     /locus_tag="VFQ35_25290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934053.1"
                     /translation="MSSSDGSFVRRSLAAVVLGCCLSSCDSAYVGSDVLWSAEHETGD
                     LSEWLRDNAGAAEVDTDDGSVAVSDAFAHTGRYSVKLEKIVAGTPARGAGPRLIRYAN
                     LPKHAFYSAWFLIPEAYATGSYWTILQFDSSAAESSVEDRGVNLQLRTLPDGNGLVLQ
                     VIFHLSAYLAAPLANPPPRVPIGRWFHLEAEFEASVEATGGLVVWLDGERIYDLRQRS
                     TVDPETLEFLVASMLVDASPSPVTLYVDDVVISRSRTGAH"
BASE COUNT          946 a         1936 c         1906 g          964 t
ORIGIN      
        1 gacggcaacc ggtggcgcgc tcgcgagcgg cggctcgacg gcaaccggcg gctcgacgaa
       61 cgaaggcggc tcgcccgcag cggccgaagg aggcacgagc acggcggaag ggggctcgac
      121 ttcttcggct gaaggcggcg gcacgagcaa tccaggcgac gtgaccggcg gcacgggcgg
      181 agccggcggc acctcagccg gctgaacgac acttggcggg ctcgtctcga tgggctaagg
      241 ctcgcccggt gctcgcttcg tctttcgccc gcgctctcgc tctgagcgcg ctcgctgcct
      301 ccctcctcgg ctgtagtagc agtgggtcca cctccactgc gctgcctcac ccgacggtcg
      361 tcgaggtatc gcccctcgaa tttgcgagcg agccggcgtg cgacgctggg cccggatcgc
      421 tgaagcggta catcgcgacc ctcgtcgacg tgactccgac gagcgcgacc gattcgaagc
      481 ccctcgactt caagctgccg agctccgcgc tgcaagtcga agccaacgga tccattcatt
      541 atgagccgat cccgtgtcgg ctctccgtta gttttggttg ggtcgtcgcc ggccatagct
      601 atcaaaccga actactcggc tacgatcggg acgatctcga gctcctcgag ccgggctttc
      661 caatcgccgt cgatccggcg agtcgcgccg tcgtcgaacc acgctggacc gccatgtgcg
      721 gagccgctag cgaggacgac tcgagcgatg gaggcagcgg caacgtcccc gagcgcgcgg
      781 ttcgcgccga gagcgacctc acccggacgg ttcgctactg cggcgagtgg ctcgagtcgc
      841 agcagtagcc ggaacggcgg caaactcggg gccaccggcg gaaccgattg cctcgaagtg
      901 agagaagcgt aacttaagct cggtgagccg agtcgaaagt ccgctacagg gcagcttgcc
      961 gtaccccggt cgagtcttgc ggatcgtgat gatcgccatc gcggcccttt ggctcgcctt
     1021 tgcgatcggg ctgaactggg ctggcgcgcc gccggagctt ttctgggcgc tctgcggcaa
     1081 caccgacaag attctgcacg gcgaggtctg gcgcctattc accgcgcctt ggcttcacct
     1141 gccggtggcc tcgctggggc acgttttttc ggctctgctc gggctgtact tcctcggcgc
     1201 gtcgctcgaa cagcagtggg gaagcgcccg cttcgcgaag ttcctcttct tcgccgcgct
     1261 cctcgcctac gtgacgcaaa tgctggcgga gctcgtgctg ccccgctcgc tcgagatcgc
     1321 gcccgccgat ggctattggt ttggcgccat tccggtcgtc gaagcgatcg cgatcgcctg
     1381 ggcgttgagc ttccgcggcc gcgaggtgcg ccttttcttc gtgctcccgg tgagctcgac
     1441 cgggctcatc ttgttcgtgg tcggcgcgag cgtcgcgtcg gtcatcatca aggcacaggc
     1501 gccgtctggg ctcgttgctc cgttcggtgg gatgctcgct ggctggctat tcgggtcggg
     1561 ctcgccttcg cccctgcgcc gcctatggct caaactgagg cttgcgcagc tcgacgtcga
     1621 agccaagcgg gaggcgcaag cgcgccgtaa ccgcgccgcg cgctccggct tgaaagtgat
     1681 aaccggtggc cgcgatgacg actccgagga cgattccaag cgcggcggtg gccgctggct
     1741 gaactgagtt ggctccgctc cgcgaacaaa ccccggcctt cccgctgcgc ccgcgcctcg
     1801 accacggttc tcgctcgcgt ttcttgcctc tcccgaccgg cggaaacttg ggacactccc
     1861 cctagcctcg gtcgcgccgt ggtataggcg gcggtcgatg catgtcctgc tcgacgctga
     1921 aggcgtggaa cgaagcctgc ggcgcatggc cggcgaaatc gccgagcgaa cgcgcggtac
     1981 gcaagatctg atgctcgtag gtatccggcg cggcggtgtc ccgctcgccg agcggctcgt
     2041 cgctcacttg cgcgagctgg agggcgacga agtgccgcgc ggcagcgtcg acatcacctt
     2101 ttatcgggac gacgcggcga cgcgcctacc aaatccacgc atcggtccga gcgagattcc
     2161 gggctcgctc gatggtcggc acgtgatcct ggtcgacgac gtgctcttca ctgggcgcac
     2221 cgtgcgggcc gccctcgacg ctttgctcga ctacggtcgg ccccggcgcg tggagctgct
     2281 cgtattggtc gagcgcaacg gtcgcgagct cccgattcaa gccgactaca tcggtaagag
     2341 cgtggagctc gaagacgacg aacgcgtcga cgtggtcgaa ctcggcggcc agctctccgc
     2401 catcgtgcaa gcggcgaacg cgccgagcct gcctcctcaa ggaatgccat gacagagcag
     2461 cgtttgcatc acctgctggg aatcgaagac ctcaacgtcg cgagcgcaga gcagctgctc
     2521 agcaccgccg aggcattctt cgaggtctcg cggcgtccga tccgcaaagt tcccacgctg
     2581 cgcggcaaga ccatcgtgaa cgtgttttac gaggcgtcga ctcgcacgcg cacgtcgttc
     2641 gaactcgctg ggaagcgcct ctccgccgac gtcgtcaaca tcagcgtttc gtcctcgtcg
     2701 acgagcaagg gtgaaacgct gcgcgacacc gcctcgacgt tggacgcaat gcggcccgac
     2761 gtgctcgttc tgcgacactc ggcttcgggc gcgcctcact acatcgcgcg ctacacgcgc
     2821 gcagcggtcg tgaacgccgg cgacggcatg caccagcacc cgacgcaagc gctgctcgac
     2881 gcgttcacga tccggcgcgc gaaaggcaaa gatctgcgcg gccttcgagt cgcgatctgc
     2941 ggcgacatct tgcacagccg cgtcgcgcgc tcgaacgccc tcctgctcgg tttgctcggc
     3001 gccgaggtac gactcgccgg gccacgcacg ttgctaccgc agcgcgccga cgcgctcggt
     3061 gttcagatct tcgatcgcat cgaaccggcg ctcgagggcg cggacgtggt catggtgctg
     3121 cgcatccagc gcgagcgtct cggggccgcc ctgctgccga gcttgcgcga gtacgcccgc
     3181 tatttcgggc tcaacgagcg gcgcctcgcc ctcgcgcgcc cgaacgccat cgtgatgcac
     3241 ccgggaccga tgaaccgggg cgtggagatc aacgaggata tcgccgacgg cgcgcgcagc
     3301 gtgatcctcg accaggtcga ggctggcgtg gccgtgcgaa tggccgtgct ctatcactgc
     3361 gcgagcgaac ccggcagcga gcccctcagc tgaagtccgc cgtccggaaa agccgaagcg
     3421 caccagctct tcgccgttca cttcaatttt ctgcgctaat cgcgcgccgc tcttctcgcg
     3481 gcagcggcag aagtgacact cgcggcgact cgacgtttcg caggaatgct cggaactccg
     3541 gcgctgcgag tcgattcctt gcgagcgtac caggagccgg ctagagaacg tgcgcaccgt
     3601 gcctgaactc tgactgctca ggtaaccaac ttacgcgagg ctctggatgc gatccttgtt
     3661 cttgagaacg tgcgccgtat cgttggtctt gggtattccc gggctcgcct gcggccccga
     3721 agagggagcc agccagcctc cgctcggcga cggtggttac acggcagaac cgctcggcgg
     3781 aagcacggct cgaggcggaa gcacgggctc gggcggcagc aagggtttgg ggggcagcaa
     3841 aggctcgggt ggcaccaaag gtctcggcgg agcccccggc gctggggggc gaggtcttcc
     3901 cacgaacact cggacgaaca cgagtaccgg cacgaacacc cgcacgagca cgagtacgcg
     3961 cacgagcacg aacacccgca cgagcacgaa cacgaggacc tgagtctcgg gcgctcacgg
     4021 cccgggcttc ggcggtgctc gctaaaccgc ccggtgatag tctcggggcg tgcgcgctgc
     4081 gacgtgcttg ctcgttttgc tctcgcccct ggcgtgcggg acaccatcgc ggagcgaacc
     4141 gggaccgatt gaaacgctgc cggccgattc gagcgcggac atggcgctcg agttcgacgg
     4201 cgtgaacgac tacgcgagcc tcggcaccgc gggctttccg ttcgcgcgac tgccgcaaac
     4261 catctccttt tggctgaacc ccgggcgcgg cgcggccgaa cagacgatca tgacgctgaa
     4321 caaggacgcg gagagcggag tgctcctcgg cctcagcaac ggcgtaccga cggcgcgcag
     4381 cgcgtactcg ggggacgtat tcgttcaggc atcgtcggcg ttgccgagtg gtgagtggca
     4441 ccatctcgct tacctgttcg acggcaccga cgcggcgccg caccacacgc tctacgtcga
     4501 cgggaaagtc gttggaaccg gcacgctgct ccccgacaag cgcacgccga acacggccta
     4561 catggggagc gacaatcacg aagagcgttg ctacggggga ctgctcgacg aggtccgagt
     4621 ctgggccgtg gcgcgctcgc ccgagctcat ccgcgcggag ctcgcgggag aagcgctcga
     4681 gcaggagcct cccgaactcg tcctttacta caccttcgac gaggcggcgg gcgcgcgcgt
     4741 ggtggatcgc tcgggccgac aaaatcacgc tttgctcggg gacggcctcg acgactacat
     4801 gccgcgaaga gtgctgtcgg gcgtcgttcg agcgcgcgcc gcgctcggca agccttgagg
     4861 ttcagcgagc gtgccgagtc tccgtgaacc cctggcgaag aggcgcaagc tagctaatgc
     4921 gcgccggttc ggctgcgact gatgacgacg tcgtcgacgt agagcgtcac cggactcggc
     4981 gaggcgtcga ccaacatgct cgctacgaga aactccagtg tctcgggatc gaccgtcgaa
     5041 cgctgtcgaa gatcgtagat gcgctcgccg tcgagccaga ccacgaggcc tcccgtggcc
     5101 tccacggacg cctcgaactc ggcttccagg tgaaaccacc gaccaatggg gacgcgcggc
     5161 ggaggattcg cgagtggcgc cgccaggtac gcgctcaggt ggaagatgac ctgcagcacc
     5221 aagccgtttc cgtcgggcag agtgcggagc tgcaagttga cgcctcgatc ttctaccgag
     5281 ctctctgccg cgctcgagtc gaactggaga atcgtccagt acgatccggt cgcgtatgcc
     5341 tctggtatta gaaaccacgc gctgtaaaag gcgtgcttcg ggaggttcgc gtaccgaatc
     5401 aagcgcgggc cggcgccgcg agcgggagtc cccgcgacga tcttctcgag tttaaccgag
     5461 tagcgccccg tgtgcgcaaa cgcgtcgctc acggcgacgc tgccgtcgtc ggtgtccacc
     5521 tccgccgcgc ccgcattgtc gcgcaaccac tcggacagat cgccggtctc gtgctcggct
     5581 gaccagagca cgtcgctgcc gacgtaggcc gaatcgcaac tcgagagaca gcagccgagc
     5641 actacggccg ccagcgagcg gcgcacgaag cttccgtcac ttgagctcac taatcggcac
     5701 cgtagtcgcc gcgcgcggcg actttgatgg tggcgctggc ggcgcttcgg cg
//
LOCUS       DASTKS010001188         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1307182, whole genome shotgun sequence.
ACCESSION   DASTKS010001188 DASTKS010000000
VERSION     DASTKS010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307182"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2682)
                     /locus_tag="VFQ35_25295"
     CDS             complement(<1..2682)
                     /locus_tag="VFQ35_25295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934054.1"
                     /translation="MQDSERATFSGETTVTFKVSAANLKGTLTSGRIVFHTTLGDVAV
                     TPTIRVGLSGSYQGVMHYESKGTSYGDVGLAVDMLDKQGDVVVRVDPERSMTFPQTSS
                     GVAAGRGVFTLSEGLSVTLTQRIDADYGGKRNHFQRPLGRRIAFKLHPSKDGGGKLSG
                     DFQETVYGVTDQPIVTAGSAYLEPKDVGHMPCPGPTAKAPCVDFTVPPAVLPVQPAGG
                     FASMTNFPGWSDAQVAAFGGCANSGAPYTDCRPGDPQCIYGADCSDQGCLDATVEKRY
                     YGLTETLGRVRTGDNPLDDIAKNCEAELQATSPDAFRAAVAQHSGSGSCGYPPALVCA
                     LRELSSANWGQRYLAGAQSTFSRIYARALAPGLFVAQNHIVEGLKESFAVGLSSEVSR
                     FEKARAAVDAPLTAALSTQTLQYLEALPLAVAAGNAKSADLTNSSYPAGRALARALYV
                     LHTLDGEQARLESTDLTVSQEDRIARAQARGVFGLIEAVTLSSLLNAWGNPTDFGNEA
                     AGSLTLADRGFRALQQGPEAFGVPEGQIPFVYDPHRSQNTNFEQMLTFYSGPRLDLAK
                     STEVEFQAATRAFEQGRDQLNAELGQVRLGIDGQVAEICGNGFVLDKPNWEECGANNA
                     GEIGSASLSIELANARLQSQYTRLDGMGKKIAIDRDALAKSQALLASDITFVKDTGEE
                     LDVVIEAQGAINAMEKGLEMAANSNLWNAGAPLAMAAFTMVLEAQRTALDVKRQNLQT
                     AQQVHAAQTSKDVAYVEGAANIQKQLIDIQQLTVDMQQDQIGVTQAQLNRANLLDKAK
                     RLMEERVRVLARISGASYSDPMFRVLQSDTALAAVQARAEAQRALYLSGRALEYELNM
                     PLGDALGRAVLKATNTSRQAALSSCLSSIHSDYVAQFR"
BASE COUNT          439 a          853 c          881 g          531 t
ORIGIN      
        1 ccggaactgc gcgacgtagt ccgagtgaat cgaggagagg cagctgctca gcgccgcctg
       61 gcggctcgtg ttggtggcct tgagtacggc gcgcccgagc gcgtcgccga ggggcatgtt
      121 gagttcgtac tcgagcgcgc gcccggagag gtagagcgct cgttgcgcct cggcacgcgc
      181 ttgcacagcc gccaacgcgg tgtcgctttg caagacccgg aacatcgggt ccgagtacga
      241 ggcgccggag atgcgagcga ggacgcgcac gcgctcttcc attagccgct tggctttgtc
      301 gagtaggttg gcgcggttca gctgagcttg cgtcacgccg atctggtcct gttgcatatc
      361 gacggtgagc tgctggatat cgatgagctg cttctggatg ttggcggcgc cttcgacgta
      421 ggcgacatcc ttgctggtct gcgcggcgtg aacctgttgt gcggtttgca gattttggcg
      481 ctttacgtcg agggccgtgc gctgcgcctc gagcaccatc gtgaacgccg ccatggcgag
      541 cggcgcaccg gcgttccata ggttcgagtt cgcggccatc tcgagcccct tctccatcgc
      601 gttgattgcc ccttgcgctt cgatcacgac atcgagctct tcgccggtgt ctttgacgaa
      661 ggtaatgtcc gacgccagca gcgcttgcga cttcgcgagc gcgtcgcggt cgatggcgat
      721 cttctttccc attccgtcga gccgcgtgta ctgcgactgg aggcgcgcgt tggcgagctc
      781 gatgctcagg ctcgcgctgc cgatttcgcc agcattgttc gctccgcact cctcccagtt
      841 gggcttatcg agaacgaatc cgtttccgca gatctcagcc acctgaccgt cgataccaag
      901 tcgcacctgg ccgagctcgg cgttgagctg gtctcggcct tgctcgaacg ccctcgttgc
      961 ggcttgaaac tcgacttcgg tggacttcgc gagatcgagc ctcgggccgg agtagaacgt
     1021 cagcatctgc tcgaagttcg tgttctgcga gcgatgcgga tcgtagacga aggggatctg
     1081 accctcgggt acaccgaagg cttccggccc ttgttgtagc gctcgaaagc cgcggtcggc
     1141 gagggtcaag ctcccggcgg cctcgttgcc gaagtcggtc ggattgcccc atgcgttcag
     1201 cagtgaagaa agcgtaaccg cctcgatcag gccaaacacc ccgcgtgcct gagctcgcgc
     1261 gattcggtct tcttggctta cggtcaggtc ggtcgactcg agccgcgctt gctcgccatc
     1321 gagcgtgtgc agaacgtaga gggcgcgagc aagcgctcgt cccgcgggat agctcgagtt
     1381 ggtgaggtcg gcgctttttg cgttgccggc agccacggcg agcgggagcg cctcgagata
     1441 ctgcaacgtt tgcgtcgata acgcggcggt aagaggcgca tcgacggcag cgcgagcttt
     1501 ctcgaagcgc gaaacttcac tggagagacc gaccgcgaag gattctttga gcccctcgac
     1561 gatgtggttt tgggcgacga ataggcccgg agccagcgct cgcgcgtaga tgcgactgaa
     1621 cgtgctctgc gcccccgcga ggtatcgctg accccagttc gcggaagaaa gctcgcgaag
     1681 ggcgcaaacc aacgccggag ggtacccgca cgaacctgag cccgaatgct gggcaaccgc
     1741 ggctcggaag gcatccggcg acgtcgcttg aagctcggcc tcgcaattct tggcgatgtc
     1801 gtcgagcgga ttatcccctg tccgcactcg gccgagcgtt tcggttagcc cgtagtacct
     1861 cttctcgacg gtagcatcca gacacccttg gtcgctgcaa tctgcaccgt aaatgcattg
     1921 tggatcgccg ggcctacagt cggtgtacgg tgcgccggag ttggcgcagc cgccgaaggc
     1981 tgcaacctgc gcgtcgctcc agccggggaa gttcgtcatg ctcgcgaagc ctcccgctgg
     2041 ctgcacggga agcacggctg gcggcactgt gaagtccacg cacggagcct tcgccgtcgg
     2101 tcccgggcac ggcatgtggc ccacgtcttt gggctcgagg taggcgctcc ccgcggtcac
     2161 gatgggctga tcggtcacgc cgtacacggt ctcctggaag tcgccgctca gcttgccgcc
     2221 accatccttc gatgggtgca gcttgaacgc gatgcgacgc ccgagcgggc gctggaagtg
     2281 gttgcgttta ccaccgtaat ccgcgtcgat ccgttgggtg agtgttaccg aaagcccctc
     2341 gctcagagtg aacacgccgc gaccggccgc aactcccgag ctcgtctgcg ggaacgtcat
     2401 cgagcgctct gggtcgactc gcaccacgac gtcgccctgc ttgtccagca tgtcgaccgc
     2461 aagcccgacg tcgccgtagc tcgtgccttt gctctcgtag tgcatgacac cttggtagga
     2521 accgctcagg ccgacgcgaa tcgtgggcgt gaccgcgacg tcacccagcg tggtgtgaaa
     2581 cacgatgcgg ccgctcgtga gcgttccctt gagattcgcc gcgctgacct tgaacgtaac
     2641 cgtcgtctca cccgagaacg tcgcgcgctc cgagtcctgc aacgacagga agggcgcgcg
     2701 aagc
//
LOCUS       DASTKS010001189         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_412099, whole genome shotgun sequence.
ACCESSION   DASTKS010001189 DASTKS010000000
VERSION     DASTKS010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412099"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..432
                     /locus_tag="VFQ35_25300"
     CDS             172..432
                     /locus_tag="VFQ35_25300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9934055.1"
                     /translation="MHRTGIGDRFSEAARLLWEEMTQRSWDQSQLAEYLGTSSGTVNR
                     WLYGERRATGDWPFVIQDKLGIDARLWGVNAKKPFEPPAARA"
     gene            1053..1550
                     /locus_tag="VFQ35_25305"
     CDS             1053..1550
                     /locus_tag="VFQ35_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934056.1"
                     /translation="MSSAAPASPYSWLGELTANSYKYRGIYRLDDTRIVATDGTAFVV
                     VVSDETAPTIECAEAIARHIGFEVEKEAVIEFRRLTDWLAALSPLAVDAGTVFGRRIN
                     RHLVKRFVGPLQAKEVMVQYRGFEQAIAFLDVDERWGVYAMPIVERRDPLGGLELGPV
                     PELIK"
     gene            1547..>2856
                     /locus_tag="VFQ35_25310"
     CDS             1547..>2856
                     /locus_tag="VFQ35_25310"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9934057.1"
                     /translation="MSYADFLARKALVDVPTGFERVPDLHPALKEHQRLLTSWGLRRG
                     RAAIFADTGLGKGWMVLEWARCVADRFGAPVLILAPLAVAQQFARESAKLGRELAICS
                     SSKDIRPGVNVTNYQKLHRFDPTEFGGVALDESSILKSLDGSTRAELIERFRDTPFKL
                     SATATPSPNDHTELGGQAEFLGVMSQAEMLATFFVHDGGSTADWRLKGHARDDFWRWV
                     CSWGAIVKMPSDLGCADAGYELPPLHVHEHLVPATVEDARAAGKLFADAAESLSEQRA
                     ARKSTLKARVELAASIANYTKDQVIVWCDLNDESRALARAIWSSEEVTGSQTDEEKEA
                     AITRFLDGRSRVLVSKPSICGFGLNLQFVHRQVFCGVTHSFEAYYQAVRRCWRFGQEY
                     PVEVHVVSSELEGRVVENLKRKQREADELASETRRFVAEHVRTVV"
BASE COUNT          522 a          877 c          910 g          547 t
ORIGIN      
        1 gaaattcatc gccttgcgga ccctgtcggc aagcgtcttt tcgtcctctg tgctgctcac
       61 aggttcactc tctgccaact ggaagggata ggtcaagccg ctcattcgct ctgacacttg
      121 acaggtctgt cactcggtag aatcataggc ttcgtggagc acattcgaac gatgcatcga
      181 acggggatcg gcgaccgttt ctcggaagcc gctcggctcc tctgggagga gatgacccag
      241 cgcagctggg accaatctca gctggccgag tacctcggaa cttcaagcgg caccgtcaac
      301 cgctggttat acggagaacg gcgcgcaacg ggcgattggc ctttcgtcat tcaggacaag
      361 ttgggaatcg acgcgcgcct ctggggcgtg aacgcaaaga agcctttcga gcctccggct
      421 gcgagggcgt gatgaacaaa tctcatgccc attcgacggc cgaggccgtc tcatctcttc
      481 acgacgagct cgacataccg acacctgaac tgacagctgt acgcgcggtg aagcgcgcgc
      541 gacttctaca cctcgtacag acgcgggtca cgacttacgg ccggctgggg cgctgctgga
      601 aggtctctcg gcaaacggca cgaaagctcg ttacgcgcga ctccggcccc ccgagcacga
      661 agctccgaac attaccaccc aaccttcaga gggagctcaa ccgatgatcg gggctgttgc
      721 aattatcgtt tgggtcgcac ttgtcattgc ggacgcgcgc cgcgggagga tgcaatgact
      781 cctctttttt tgactcattc cgaaatcaaa cagatggatc tcgccggagg cgaagctatc
      841 gctcgaatcc cgctcggaca accggacaca agcacgaata cgggtgttca tctgacgctc
      901 gtcctagaat cctgtgcgtg cgggacgaag ctgtgtggtg cagcgggcgg cgtccgagag
      961 gtctcgttca atctcgatgc gcgataccga gggcccgagc actttcgact caatgagcgc
     1021 gagctctttg ccctggcgca tgcggggtgc tcgtgagctc cgcggcgccc gcttcgccgt
     1081 actcgtggct tggggagctc actgcgaatt cgtacaagta tcgcggcatc tatcggctcg
     1141 acgacacgcg catcgttgca actgacggca ccgccttcgt cgtcgtggtg agtgatgaga
     1201 ccgcgccgac catcgaatgc gcagaggcca tcgcgcgaca catcggtttt gaagtcgaaa
     1261 aagaggcagt tatcgagttt cgtcgattga ccgattggct cgcggcgctt tcgcctcttg
     1321 ccgtcgacgc gggcaccgtc tttggtcgac gaatcaatcg ccatctggtc aagcgcttcg
     1381 tcggccccct ccaggcaaaa gaggtaatgg tacagtaccg aggcttcgag caggccatcg
     1441 cctttctcga tgtcgacgaa cgatggggcg tttatgcgat gccaattgtc gagcggcgcg
     1501 atccgctcgg cgggctcgag ctcggacccg taccggagct catcaagtga gctacgcgga
     1561 ctttctcgca cgcaaggcgc ttgtcgacgt gcccacgggc ttcgagcggg tgcctgacct
     1621 gcacccggct ctgaaggagc accaaaggct gctcacgtcg tgggggctcc gccgcgggcg
     1681 agcggccatc ttcgccgata cggggctcgg caaggggtgg atggtcctcg agtgggctcg
     1741 gtgtgtcgcc gaccggttcg gcgcgcctgt gctcatcctc gcgccgctgg cggtcgctca
     1801 gcagttcgcg cgcgagtcgg cgaagctcgg gcgcgagctc gccatctgtt cgagctcgaa
     1861 ggacatccga ccgggcgtca acgtcacgaa ctatcaaaag ttgcaccggt tcgacccgac
     1921 cgagttcggt ggggtggcgc tcgacgagtc gtcgattctc aagagcctcg atgggtcgac
     1981 ccgcgcggag ctcatcgagc ggtttcgcga cacgccgttc aagctgtcgg cgacggcgac
     2041 gccttcgccg aacgaccaca ccgagctcgg tgggcaagcc gagtttctcg gggtgatgag
     2101 ccaagccgag atgctcgcca cgttcttcgt gcacgacggg ggctcgacgg ccgattggcg
     2161 cctcaagggg cacgctcgcg acgacttttg gcgatgggtc tgctcgtggg gagcgatcgt
     2221 caagatgcct tccgaccttg gatgcgcgga cgccggctac gagctgccac cgctccacgt
     2281 gcacgagcac ctcgtgccag cgacggtcga agacgctaga gccgccggca agctcttcgc
     2341 cgacgccgcc gagagtctct cggagcagcg tgccgcacgc aagtcgacgc tcaaggctcg
     2401 tgtcgagctc gcggcgtcga ttgcgaacta cacgaaggat caggtcatcg tctggtgtga
     2461 cctcaacgac gagagccgtg cgcttgcgcg cgccatctgg tcctctgaag aggtcaccgg
     2521 gtcgcagacg gacgaagaga aggaagcggc catcactcgc tttctggatg gtcgctcgag
     2581 agtgctagtc tcgaagccgt cgatctgcgg cttcggcctg aaccttcaat tcgtacaccg
     2641 gcaagtcttc tgcggggtga cgcattcctt cgaggcctat taccaggcgg tgcggcgttg
     2701 ctggcgattc ggccaagagt acccggtcga ggttcacgtc gtctcatcgg agctcgaggg
     2761 gcgcgtcgtc gagaacctga agcgtaagca gcgcgaggcc gacgagctcg cgagcgagac
     2821 caggcgcttc gtcgccgagc atgtgcgcac ggtcgt
//
LOCUS       DASTKS010001190         9245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_190280, whole genome shotgun sequence.
ACCESSION   DASTKS010001190 DASTKS010000000
VERSION     DASTKS010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190280"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFQ35_25315"
     CDS             <1..850
                     /locus_tag="VFQ35_25315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934058.1"
                     /translation="LRVLVGVAALVMVERVAFAQSPPLCIPGTPLCARTTANGGVQVG
                     GGAQGQAGPGGVTGDVHGNANANGNANASGNANASGNAGGSGRGGVGGGLSGSGNGSG
                     SGSSSGSGSGGGGSGGGGFASYDSTPTRWGAGLALCGSVKAGIYSGVKWGPCFAVSFR
                     FESLAFEMESQLLFGGVRHSVDWVFPMSFVIPLTEPRKLYQGLDLRVGGSPIGVTFAK
                     SKDGGSYLRFGLHAGLSYEYDLGPAASWRVFDVRAFLDFGTKREVDLHDNFIDFGGQL
                     ATGIVF"
     gene            complement(957..1352)
                     /locus_tag="VFQ35_25320"
     CDS             complement(957..1352)
                     /locus_tag="VFQ35_25320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934059.1"
                     /translation="MGFARIGWAFFVVAALVGCWDNDPCDAGQIFKDGVCFQAPSASG
                     GSTSSGGQTGDAGSGGDSSGPAQPDSWAKQCTQPADCTGNSPICAPAPLGYCTNINCS
                     PGEANEGICPAGWTCFPAGGGNPSACLKL"
     gene            complement(1379..1837)
                     /locus_tag="VFQ35_25325"
     CDS             complement(1379..1837)
                     /locus_tag="VFQ35_25325"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9934060.1"
                     /translation="MKRRILLFALLGSALFWQAESSAGDDVIAVIANKDVPQNKLSRD
                     DLRPIFQATKTQWSNGATIVPLNLPEDNSMRKGFDSAVLGLDPDRVARYWIDRKIRGD
                     ARPPKKLSSPGAVLKAVGKTGGAIGYVALGDVDASVKVVAKIKGGNVVAP"
     gene            complement(1839..3317)
                     /locus_tag="VFQ35_25330"
     CDS             complement(1839..3317)
                     /locus_tag="VFQ35_25330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934061.1"
                     /translation="MRSLSKGIRGSALVLSLSISAHVARAQEASPGGTEAPAAAPAES
                     PAAAEAPATAPPKAAEAPPASEPNKEIEELKKRVDEQGTQIEELKSAQDEAAAAAEES
                     AQPQKLNIYGFMDMGFQRAWTKPESPLSNFLNANAATFVIGNLNVYFDAQPIKGWRGL
                     MEVRFTNAPLGNIESVGGLGGTFKRTDTQQIDPHGASLNAPMWGGYTVIERAHIDWMP
                     RQEFNLRVGNFFTPFGIWNVDHGSPTLISTALPQMIVQRMFPIRQTGLMVFGSTMAGP
                     WELGYTATLTNGRQEEANFDFDDDKAFGARVYARNDGKIRSMMGLSFYEGDTNDKVVS
                     ITAVQPSLQFKTEESYRYKEWSAGADLSLDIGDSRIRAEAAVRRVKYDPGKRTKADPL
                     FSAGGFSPDAYYSTAYLIWAHQLPWAGIEPFVFGEVMRGTFGISDSIATLSGGVNVHF
                     TPTTMLKTQVTRALFFNQYSSSADNPSQHDTTVLFTRLVLAY"
     gene            3716..6409
                     /locus_tag="VFQ35_25335"
     CDS             3716..6409
                     /locus_tag="VFQ35_25335"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET9934062.1"
                     /translation="MLEKLKKIGVKLAILAGVPVIGALLLSAHITREANERARSSEGI
                     GSVEDLAELSARMTATIGELQSERALGALGAGFENPDRSSLVAQEAKTDAAVHAMDDF
                     LAKRDLTRLPRRLGSDLSRARAQLRQLAGQREKLAQPKASIDDVLSFYGETNNELINA
                     SAALTQLSDDGELLRALSTLVATMQVKERSSREHAVLNNTFAHREFAPGLYRYFVTLV
                     TEEAVYMASLKSLASDEQSRLFEHALEGPLVEKSGAMRKKALETTDDEFGIEAAEWDQ
                     TQARKVAALSAAERELAHRARSVAKRKVAETQGALAYSRNLVIAVVAVSLLLAWIIGR
                     GISRSVLGLARVAGRVQKEQDFSLRAEKTSQDELGALTDAFNEMLSGIQVRDKELESH
                     RENLEALVTERTRELSQRNEDMRLVLDTVDQGLVTLQKDGSISNERSRAFDQFFGSPP
                     PGLPYYEHLAGDDENLRLYLKLDWEQLTEGILPIEVVLDQAKNRIERAGMHYALAYKP
                     IVKDDQVTGALLMVSNVTNEMAARKAEAAQREQIKTIARVLKDRQGFLEFFSEARKLL
                     ERIRDDEFVSRAEKLRGVHTLKGNAALFDVSSVAEAAHGLEQALVDDEDGAQIAELTL
                     ALEMAWDNFAQRIVPVLGDDIGERFEVTRAELDELRGATKPFPELAKLVERIEHEPVR
                     MRLVRASEQLTSLSQRLGKADVDYELSAEGVRLASARFAPFWVAFAHVVRNVADHGLH
                     TREERESLGKPPHNRVELTARQVGGNVVVEVADDGRGVDWEKVRVRAQKLGLRHETRA
                     DLVAALFAPGLSTAEELSSTSGRGVGMSAVAEACRALGGTFTLESELGKGTRMSFTLP
                     ITSAERPEPRPRLSMAPVTIPPRRNSHRPLHHRPSAPPPAD"
     gene            complement(6449..6931)
                     /locus_tag="VFQ35_25340"
     CDS             complement(6449..6931)
                     /locus_tag="VFQ35_25340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934063.1"
                     /translation="MYEAGNQGEGFGAGGYAMGYSPAQQAEWKQRIIDDEQLRLLRIG
                     YFISAGQAALFIPLGLLYAGMGVFFSVMPPGASSSPPPGSEMTWIFAAFGGVVTLIAT
                     AMVALKFMTAVRLKARRSRMLCMVSAALCFIEIPYGTALGLMTFLVLARSSVKAQFEE
                     "
     gene            complement(6978..7736)
                     /locus_tag="VFQ35_25345"
     CDS             complement(6978..7736)
                     /locus_tag="VFQ35_25345"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934064.1"
                     /translation="MRWTDDGWLSTLAGDALPSLLEDAPRTVDALPLPEEPAARAFEG
                     GELPIEFQWLRCPEPSELFSLEARPGHLRLFGRESIGSLFHQSLVARRQTSHSFSATM
                     RFEFEPEHFQQSAGLVCYYNGSKFHYLFVSRDDVHGKYLRVHSCLPDQLLTDVFSDFV
                     PIETGKRIELRVEVDRERLQFAFRLEGEPWRFIPQTFDASILSDEAGPPGPPNFTGAF
                     VGMSCQDLAGTRCAADVADFSYRDRAFQGPPWAP"
     gene            complement(7722..8633)
                     /locus_tag="VFQ35_25350"
                     /pseudo
     CDS             complement(7722..8633)
                     /locus_tag="VFQ35_25350"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
     gene            8734..>9245
                     /locus_tag="VFQ35_25355"
     CDS             8734..>9245
                     /locus_tag="VFQ35_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HET9934065.1"
                     /translation="MYLTRHETSRGPRWALGAHWLPEAFELRMALQVPASSLRSLLQT
                     LVTKEAASGPLLPPVEPSQEVWASGVTYLRSREAREAESTQRDVYAKVYDAKRPELFF
                     KSTGARVVGHQMPIRVRADSQWNVPEPELTLLLDSRGEIVGYTVGNDVSSRDIEGENP
                     LYLPQAKVYDG"
BASE COUNT         1684 a         2888 c         3055 g         1618 t
ORIGIN      
        1 ccttcgtgtg ctggtcggag tcgcagcgct ggtcatggtg gagcgagttg cgtttgctca
       61 gtccccgccg ctctgcatcc ccgggacgcc gctctgtgcg cgcaccaccg cgaatggcgg
      121 cgtgcaggtc ggcggcggcg cgcaggggca agctgggccc ggcggagtaa cgggcgacgt
      181 tcacggcaac gcgaacgcga acggcaacgc caatgcgagc ggcaacgcga acgcgagcgg
      241 aaacgccggt ggttccgggc gaggcggggt gggaggaggc ctttccggat ccgggaatgg
      301 ctcaggctct ggtagctcgt ccggttcggg ctccggcggt gggggcagcg gaggcggtgg
      361 tttcgcgagt tacgattcga ctccaacgcg ctggggcgca gggctcgccc tctgcggcag
      421 cgtcaaagcc ggcatctact ctggtgtgaa gtggggccct tgctttgcgg tgtcgtttcg
      481 cttcgagtcg ctcgcgttcg agatggagag tcagctcctg ttcggcggag tgcggcactc
      541 ggtagattgg gtgttcccga tgtccttcgt gatcccgctc accgaacctc gaaagctgta
      601 ccaagggctg gacttgagag tcggcgggtc gcccatcggt gtgacgttcg cgaaatccaa
      661 agacggtggc agctacctgc gcttcggatt gcacgcgggg ctctcatacg agtacgacct
      721 cggtccggcg gcgtcctggc gtgtcttcga tgtgcgcgcc tttctcgact tcgggacgaa
      781 gcgcgaggtc gacttgcacg acaacttcat cgattttggt ggtcagctcg ccaccgggat
      841 cgttttctag tcgagcggcc gtctagcgag tgtgcgggag ctcacttggc acgacgctgc
      901 gcacgccctc cgcgtcgagc cgccgccgct gcgacatcga gcgcgccgct ctcccgctaa
      961 agcttgagac aggcggaggg gttgccgccg ccagcaggga agcaagtcca ccccgcggga
     1021 caaatgccct cgttggcttc accgggtgag cagttgatgt tggtgcagta gccgagcgga
     1081 gcgggcgcac agatgggtga gttgccggtg cagtcagccg gctgcgtgca ttgcttggcc
     1141 cagctgtcgg gctgagccgg ccccgaagag tcgccgccag agcctgcgtc gccggtttgc
     1201 ccgccgctcg acgtcgaacc gcccgaagcg ctcggcgcct ggaagcagac accgtccttg
     1261 aaaatctgtc ccgcgtcgca cgggtcgttg tcccaacatc caaccagggc cgcgaccacg
     1321 aagaaagccc agccaatacg tgcgaatccc atgtgcttgt tcatttcgcg tgttccgttc
     1381 acggtgcgac cacgtttccg cccttgattt tggcgacgac tttcacgctc gcgtcgacgt
     1441 caccgagcgc gacgtatccg attgccccgc ccgtcttccc gaccgccttg agcacggcac
     1501 cggggctcga caacttcttc ggagggcgcg cgtcgccgcg gattttgcga tcgatccagt
     1561 agcgggcgac gcgatcggga tcgagcccga gcacggcgct gtcgaagccc ttacgcatac
     1621 tgttgtcttc tggaaggttg agcgggacga tcgtcgcgcc gttcgaccac tgcgtcttcg
     1681 ttgcttggaa gatgggccgc agatcgtcgc ggctgagttt gttttgcggg acgtccttgt
     1741 tggcgatcac cgcgatgacg tcgtcaccgg ccgagctctc tgcctgccaa aagagagctg
     1801 aaccgagcaa tgcgaagaga aggattcgcc gtttcatgtc agtacgccaa gacgagacgg
     1861 gtgaacaaga cggtcgtgtc gtgctgcgac ggattgtcgg cgctcgagga gtattgattg
     1921 aagaacagag cccgcgtgac ctgcgtcttc agcatggtcg tgggagtgaa atgcacgttg
     1981 acgcctcccg acagggtcgc gatcgagtcc gagatcccga aggtgccgcg catgacctcg
     2041 ccgaaaacga acggctcgat cccggcccaa ggcagctgat gcgcccagat taggtacgcc
     2101 gtgctgtagt acgcatccgg tgagaaaccg cccgccgaga agagcgggtc agccttcgta
     2161 cgtttaccgg gatcgtattt cacgcgacgg accgcggctt cggcgcgaat tcgcgagtcg
     2221 ccgatgtcga gcgacagatc cgcgcccgcc gaccattcct tgtagcgata gctctcttcg
     2281 gttttgaatt gcaggctcgg ctgcaccgcc gtaatgctga cgaccttgtc gttggtgtca
     2341 ccttcgtaaa acgacaagcc catcatcgag cggatcttgc cgtcgttccg agcgtagacg
     2401 cgcgcgccga aggctttgtc gtcgtcgaag tcgaagttcg cctcttcctg gcgcccgttg
     2461 gtcagcgtcg ccgtgtaccc gagctcccac ggcccggcca tggtgctgcc gaagaccatc
     2521 aaaccggtct gacggatggg gaacatgcgc tgaacgatca tctgcggcag cgccgtcgag
     2581 atgagcgtag gagaaccgtg gtcgacgttc caaattccga acggagtgaa gaagttgccg
     2641 acgcgtaggt tgaactcctg gcgcggcatc cagtcgatgt gggcgcgttc gatcacggtg
     2701 tagccgcccc acatcggcgc gttcagcgaa gcgccgtgag ggtcgatttg ctgcgtgtcc
     2761 gttcgcttga aggttccgcc cagaccgccc acgctctcga tgttcccgag cggtgcgttc
     2821 gtgaagcgca cctccatcaa gccgcgccag cccttgatcg gctgcgcgtc gaagtagacg
     2881 ttgaggttac cgatgacgaa cgtggccgcg ttcgcgttga ggaagttcga caagggcgac
     2941 tcgggcttgg tccacgcccg ctgaaagccc atgtccatga agccgtagat gttgagcttc
     3001 tgcggttgag cgctctcctc cgctgcggcg gccgcctcgt cttgagcgga cttcagctct
     3061 tcgatctggg ttccctgttc gtcgacgcgc ttcttgagct cctcgatttc cttgttcggc
     3121 tcgctcgcgg gcggtgcttc tgccgccttg ggcggagccg tcgcgggagc ttccgccgcg
     3181 gcgggagatt ccgccggtgc ggcggcggga gcttccgttc cgccaggcga ggcctcttgc
     3241 gctcgcgcaa cgtgcgcgga gattgataat gagagaacga gagcggaccc ccgtatcccc
     3301 ttcgacaagc ttctcatttt gctcattttt cacgaacgaa tggtcgttcc gtggagctcg
     3361 aacgtcacgc aagcccgctt gcttaagaga gcgtacgtga tcaaatcggt acgtagccag
     3421 aactcgaaag ctcgcgaaca tcacttcgcc gaatcgagag tcgcaattcg tcacgttggt
     3481 acggacccct cacacaagtc tggttacgct acgagagcgc ggccgttcga gtgcatcgca
     3541 actcgcgctc gcgcgtgcat cgatcccaag cggacagaac ggggcaattt ggccgtgtaa
     3601 ggtcgtgcac gcccttcgag cccttcagtc gacggaattc ctgccgcttc tagggctgcc
     3661 gccacacccg tgttgtccgt aatttacacg acacgccact ccgcggaccc gtcgcatgct
     3721 cgaaaaatta aagaagatcg gcgtcaaact cgcgatcctc gcgggagtac ccgtgatcgg
     3781 cgcgctgctg ctttccgctc acatcacgcg cgaggcgaac gagcgcgcgc gcagcagtga
     3841 gggaatcggg tccgtggaag atctcgccga gctctcggcg cggatgaccg cgaccatcgg
     3901 cgagctccag tcggagcggg cgctcggtgc gctcggggcc ggcttcgaaa accccgatcg
     3961 gagctcgctc gtagcgcaag aagccaagac cgacgcggcc gttcatgcca tggacgactt
     4021 tttggcaaag cgtgatctca cgcgattgcc gcggcggctc ggcagcgatc tctcgcgtgc
     4081 gcgcgcgcaa cttcggcaac tcgccggaca gcgcgaaaag ctcgcccagc cgaaggcgag
     4141 catcgacgac gtcctgagct tctacggcga gaccaacaac gagctcatca acgcgagcgc
     4201 cgcgctcacc cagctgagcg acgacgggga gctattgaga gcgctctcga cgctggtcgc
     4261 gaccatgcaa gtgaaggagc ggagttcgcg tgagcacgcg gtgctcaaca acaccttcgc
     4321 gcaccgcgag ttcgcgcccg ggctctaccg ctatttcgtg acgctggtga ccgaagaggc
     4381 cgtctacatg gcttcgttga agagcctcgc gtcggacgag caatcacgtc tgttcgagca
     4441 cgcgctcgaa ggaccgctcg tagagaaatc cggggcgatg cgcaaaaagg cgctcgagac
     4501 caccgacgac gagttcggga tcgaggcggc cgagtgggat cagacccaag cgcgcaaggt
     4561 cgcggcgctg agcgcggccg aacgagagct cgcccaccgc gctcgcagcg tggcgaagcg
     4621 aaaagtcgcc gagactcagg gtgcgctcgc gtattcgcga aatttggtca tcgccgtggt
     4681 cgccgtgtcg ctcctgctcg cttggatcat cgggcggggc atttcgcgca gcgtgctcgg
     4741 gttggcgcgc gtcgcgggcc gtgtacagaa ggagcaggac ttctcgctgc gcgcggagaa
     4801 gacgagccag gacgagctcg gcgccttgac ggacgccttc aacgaaatgc tctccggcat
     4861 tcaagtgcgc gacaaggagc tcgagtcgca tcgagaaaac ctcgaggctc tcgtgaccga
     4921 gcggacccgc gagctgtctc agcgtaacga agacatgcgg ctcgtgctcg acacggtgga
     4981 tcaaggtctg gtcacgctcc agaaagacgg ctcgatctcg aacgaacggt cgcgcgcgtt
     5041 tgaccaattc ttcggctcac cgccgcccgg gttgccctac tacgagcacc tcgccggtga
     5101 cgacgagaac ttgcgccttt acctgaagct cgactgggag cagctgaccg aaggcatact
     5161 gcccatcgag gtcgtgctcg atcaagccaa gaaccgcatc gaacgcgctg gaatgcacta
     5221 cgcgctcgct tacaagccca tcgtcaaaga cgaccaagtg acgggcgccc tgttgatggt
     5281 gtccaacgtc acgaacgaga tggcagctcg caaggcggag gccgcccaac gtgagcagat
     5341 caagacgatt gcgcgcgtgc tgaaggaccg ccagggattt ttggagttct tcagtgaagc
     5401 tcgcaagctc ctcgagcgta ttcgcgacga cgagttcgtt tcacgcgccg agaagctgcg
     5461 cggcgtgcac acgctgaagg gcaacgccgc tttgttcgac gtgagcagcg tggcggaggc
     5521 cgcgcacggc ctcgaacagg ccctcgtcga cgacgaggac ggggcccaaa ttgcggagct
     5581 cacgctcgcg ctcgaaatgg cttgggacaa cttcgcccag cgcatcgtcc cggttttggg
     5641 cgacgacatc ggggagcgct tcgaggtcac tcgcgccgag ctcgacgagc tccgaggcgc
     5701 caccaagccc ttcccggagc tcgcgaagct tgtcgagcgt atcgagcacg aaccggtgcg
     5761 aatgcggctc gtgcgcgcgt cggagcagct cacgagcctc tcccagcgcc tgggcaaagc
     5821 cgacgtcgat tacgagctca gcgcggaggg cgtccggctg gcgagcgcgc gcttcgcccc
     5881 gttctgggtg gcgttcgcgc acgtggtccg aaacgtcgcg gatcacggtc tccacacgcg
     5941 cgaggagcgc gagtcgctcg ggaagccgcc gcacaaccgc gtcgagctca cggctcgaca
     6001 ggtcggcggc aacgtggtcg tcgaagtcgc ggacgacggc cgcggcgtcg actgggagaa
     6061 ggtgcgcgtg cgcgcccaga agctcgggtt gcgtcatgag acgcgggcgg acttggtcgc
     6121 ggcgctgttc gcgccggggc tctcgaccgc cgaagagctt tcgagcacct ccggtcgcgg
     6181 cgtcggcatg tccgcggttg cagaggcgtg tcgggcgctc ggtgggacgt tcacgctcga
     6241 gagcgaactc ggcaagggga cgcgcatgtc cttcaccttg cccatcacga gcgccgaacg
     6301 acccgaacca cgccctcgac tcagcatggc gccggtcacg atcccaccgc gtcgcaattc
     6361 gcaccggccg ctacaccacc ggccttcggc gccgcccccc gcggactagg cccaagttcc
     6421 cgcccgtcgc gagagccaag aagcggcgct actcctcgaa ctgcgctttc acgctgctcc
     6481 gcgcgagcac gaggaaggtc atcaacccga gcgccgtgcc gtacgggatc tcgatgaagc
     6541 agagcgcggc ggagaccatg cagagcatgc gcgagcgacg cgccttgagt cgcactgcgg
     6601 tcatgaactt gagcgccacc atcgcggtcg caatcagcgt tacgacccca ccgaaggccg
     6661 cgaagatcca cgtcatctcc gaaccaggag gaggagagga gctcgcgccc ggaggcatga
     6721 ccgagaagaa cactcccata cccgcgtaca gcaacccgag cggaatgaag agcgccgctt
     6781 gccccgccga gatgaagtac ccgatgcgga gcaggcgcaa ttgctcgtcg tcgatgatgc
     6841 gctgtttcca ttcggcttgc tgggccggcg aataacccat cgcatagccc ccagcgccga
     6901 atccctcgcc ttggttgccc gcttcgtaca tcgaaattga agctagagcc gtcctcgggc
     6961 gagaacaatg atttcgttca aggcgcccac ggcggtccct ggaacgctcg atcgcgatag
     7021 ctgaaatctg cgacgtctgc cgcgcagcgc gtccccgcga gatcttggca gctcattccg
     7081 acgaaagcac ccgtgaagtt cggtggcccg ggaggccctg cttcgtcgga gagaatgctg
     7141 gcgtcgaacg tttgcggtat gaaccgccag ggctcgccct cgaggcggaa cgcgaactgc
     7201 aggcgctcgc gatccacctc gacgcggagc tcgatgcgct tgccggtctc gattgggacg
     7261 aagtcactga acacgtcggt gagcagttgg tccggaaggc acgagtgcac ccgcaggtac
     7321 ttgccatgga cgtcgtcgcg cgagacgaac agatagtgga acttggagcc gttgtagtag
     7381 cagacgagac ccgctgattg ctgaaagtgc tcgggctcga actcgaaccg catcgtcgcg
     7441 ctgaagctgt gtgaagtctg gcgtcgcgcg acgagcgact ggtgaaagag gcttccgatc
     7501 gattcgcgac cgaacaagcg caggtgccct ggccgcgctt cgaggctgaa gagctcgctg
     7561 ggctcggggc aacgcagcca ctgaaactcg atggggagct cgccgccctc gaaggcgcgc
     7621 gcagcgggct cctcggggag gggaagcgcg tcgacggtgc gcggcgcgtc ttcgagcaaa
     7681 gaagggagag cgtcgccggc gagcgtggag agccagccgt cgtcggtcca acgcatcttc
     7741 tgaatcgcgg tttcacgccc caacgtgcag cgcccgcgac cgggcaacgg gcggccacag
     7801 agatagacca tgtaagtctc gccgtccggc gtttcgacca gatcggcgtg cccggcgcgc
     7861 gcgagctcgg cgtcaggacg atcgcgtgac gacatgacgt agccgtgggg ggacacctcg
     7921 taggggccgc gcaggttctt cgagcgggcg agggtgaccg cgtgaccgaa gccggtgccg
     7981 ccctccgccg tgatcaagta gtaataacca ttgcggcggt agaggtgagg cgcctccgtg
     8041 aaaccgagct cggttccttc gaaaatgaga tggcgctctc cgaccaagcg gcgctcgcgc
     8101 agcgagtatt cctggaggac gatccccgca aagcgcttgc agccgggccg atggtcccag
     8161 agcatgttga ccaggtattt tcggccgtct tcgtcgtgga agagcgaagg gtcgaacccg
     8221 ctgctgttca ggtaaacggg ctccgaccaa tcgccgtcga tgctcgggct cgtgacgagg
     8281 tagttgtgaa agtcgcgcag cgagacgctg gtggctcctc cttgcgtggt gcggccatag
     8341 cgcttcacgt ccgtatagat caggtaaaaa atgccattcg agtgggtgag gcagggggcc
     8401 taaaccccgc acgaatcggg cacgccggtc agatcgagct ggctcttgcg ccgtaggggg
     8461 cgggtcaaga ggcgccagtg caccaagtca cgcgagtgat ggatctgcac gcccgggtac
     8521 cactcgaagg tagacgtggc gacgtagtaa tcggagccca cgcgaacgat cgagggatcc
     8581 gggttgaacc caggcaagat tggatttcgg attctcgcgc gttcggtcgg catggttcgg
     8641 cgggagccta ccgcgcgagc tctgttcggt ataggtttgt gccggctttc gcgccgaaag
     8701 cgcttcgaga caacccgaga gaacggagcc gagatgtacc tgacccgaca cgagacgagc
     8761 cgcggaccgc gctgggctct tggcgcgcat tggctgcccg aggcattcga gctccggatg
     8821 gcgctgcaag tgccggcaag cagcttgcgc tccctgctgc agacgctcgt gacgaaagag
     8881 gcggcgagcg ggccgctgct cccgcccgtt gaaccaagcc aagaagtctg ggcgagcggc
     8941 gtcacgtatc tgcgtagccg cgaggcccgc gaagccgaat cgacgcaacg cgatgtgtac
     9001 gccaaggtct acgatgcgaa gcgtcccgag ctcttcttca agagcacggg tgcgcgcgtc
     9061 gtcggtcatc agatgcccat tcgggtgcgc gccgacagcc agtggaacgt ccccgagccg
     9121 gagctcaccc tgctgctcga ctctcgcgga gagatcgtcg gctacacggt gggcaacgat
     9181 gtctcctcgc gggacatcga aggcgaaaat ccactctatc tcccgcaggc gaaggtctac
     9241 gacgg
//
LOCUS       DASTKS010001191         6795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1307998, whole genome shotgun sequence.
ACCESSION   DASTKS010001191 DASTKS010000000
VERSION     DASTKS010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307998"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4602)
                     /gene="uvrA"
                     /locus_tag="VFQ35_25360"
     CDS             complement(<1..4602)
                     /gene="uvrA"
                     /locus_tag="VFQ35_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9934066.1"
                     /translation="MLPTRLEGACTHNLDHVSLEIEPGTLIVLTGVSGAGKSSLAMDT
                     LYAEGQRRFVESFSPYARQFLERLERPPVQRLEPVPAGVAVDRRAPIKSSRSTLATMA
                     DLEPYLAALFLREARPVCPEHGVAAEELDVKSAAARVTRVLGRDRAIVTYPLAVQSAE
                     SYLEVRDGLTRQGFRRMWVEGALVDIDQLAPSRAVTAKDSLAVVLDRVNGHDEVRISQ
                     GIETAWTHGSGVCVVRSEQRELTLRRGLGCPECGRSLPEARPGLFSYESPLGACPECR
                     GFGRTLGVDFSKVIPDPERSLAKGAIRPWRGASTTWERSELKKFCARHKIPMTEPFRS
                     LTAKQQKLIIEGEGSYDDGKFPGVAGWFRWLETRTYKMHVRVLLSRYRSYDLCHACHG
                     RRLNPTALSYHVRGKSIADLHEMEVRTARRWLSELKPGTPQGELVRRELESRITYLER
                     VGLGYLTLDRQARTLSGGEAQRVTLTAALGTSLNNALFVLDEPTVGLHPSDVEPLTTL
                     LRELAARNNSVLVVEHDPALIAAADRVIELGPGAGSQGGKVVHDAAPSALRKTKGATA
                     RALAGLAPRPRPARKPRGFLEVVGASENNLKDVSVKIPLGVVCALTGPSGSGKSTLAV
                     DIVHRALGRRLGELDLEQPGAHTRIDGAEHIKSITLVDQAPLGRTSRGNAATYTKAWD
                     AIRALYAKQPEAQARGFSAGDFSFNVEGGRCEACSGEGAETVEMQFLADVRLTCPVCK
                     GKRFKDEILAIERNGVTIADVLDMTVDRALEVFASEVAVARALGPLSMLGLGYLRLGQ
                     PLTTLSGGEAQRLKLSRALGEKHQGALYVLDEPSAGLHVDEVERLVEALDRIVDAGGS
                     VLVVEHDLDVVSRADFVIDMGPDAGEGGGSVVATGTPEQLQKKDTRTGRALASRFEPV
                     ATKAVVKAPPPSGRQRKATRAVLSVESAREHNLRDVSVQIPHAALTVVTGPSGSGKST
                     LAFDVVFAEGQRRFLETLTPYARQFLPTMPRPDVDAVTGVPPSIALEQRTARAGGKST
                     VATVTEVAHFLRLLYAKLGTLHCPTHDLPIGGMSNDALVEAVRAVKGKPYLLAPVVQA
                     RKGVYLDIFSAAARAGIEHAFADGELVKTDNPPRLVRNREHTIDLIIEPPGDAARFSQ
                     ASLNAALRWGKGALKLRSESGKEQLLSTTSACPVCAFSLPELDPRWFSFATKQGRCER
                     CEGDGVLVEERVTGRGKNKVTRTVTSTCPDCGGSRLAPIPRGVRVQNERYHEFSARAV
                     GAALERVRSLSFSGAEERIGGPLLKELERRLAFLVDVGLDYLSLDRAAHSLSGGEMQR
                     LRLAAQLGAGLTGALYVLDEPTIGLHPRDTSRLIRNLERLVELGSTVLVVEHDAETIR
                     AANYLVDMGPAGGSRGGRLVAAGAPEVVLENPESPTGRALSERAELRAPLAINESTPR
                     LRLEGVTAHNLKNVDLSIPLGRFNVVCGVSGSGKSTLVGKVLLPALRQKLGLVAEEPG
                     THSRLVGFEPLSRAVAVDQLPIGRTPRSVPATFLGIWDEI"
     gene            4763..5245
                     /gene="smpB"
                     /locus_tag="VFQ35_25365"
     CDS             4763..5245
                     /gene="smpB"
                     /locus_tag="VFQ35_25365"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9934067.1"
                     /translation="MARAKAAAGGGDRLVSKNRRAFFDYEISDTFEAGLVLIGSEARS
                     LRENSTDLTDAWIDIDGRGEAWVKGMRIPPLKHAAFGHADVRPRKLLLHRGQIDRMRG
                     LTERDGMTLIATKCYIKNNHAKLEVAVAKGKKQHDKRQSIKDKEMDREARAAIRRGLR
                     "
     gene            5242..5499
                     /locus_tag="VFQ35_25370"
     CDS             5242..5499
                     /locus_tag="VFQ35_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934068.1"
                     /translation="MSREVPLVRGGVGHIESADAQHVVVDIGVPSPPGSTVELLVANA
                     PLGVKVRSCRRLPDVEPTRFRVEGRWISLSRVQREALGIEL"
     gene            5572..6501
                     /locus_tag="VFQ35_25375"
     CDS             5572..6501
                     /locus_tag="VFQ35_25375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9934069.1"
                     /translation="MVVRGRALGLASAALAFSTMLGASSASAFCRSRACEFDKPASVP
                     CKRDSSGCPTNGAELYWPAPSFDMWVDHKGSPKYGISGEQLLDLTQEALGKWTNVDCS
                     SASPSIAVAKVELMQDDAAVAAGLVQPQKATSTDPAVTTSVVAFIDEGWMKESADAIA
                     LTTASFGTQSGRIFGADIELDSTDWRFTLDGDPSPQYDLLSVLTHETGHVFGLADLRT
                     PGPTMYGSYGEVGNTDKRSLETDDEQGMCAIYPPGRFDKDGGCGCRLEGRTRPHPGTW
                     VGVGLAALGLVLRRRRARRGDQGVNTTPNAPPK"
     gene            complement(6462..>6795)
                     /locus_tag="VFQ35_25380"
     CDS             complement(6462..>6795)
                     /locus_tag="VFQ35_25380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934070.1"
                     /translation="AWLELDVGPALRLHPGPRLDVDLAALVAVASVHVSGVRAVDEIE
                     SQRDSWSARAGVAVRLQPRLARWARLSLGAEAGFTLRPVPIEHLDVRRESLGGFYLGG
                     ALGVVLTP"
BASE COUNT         1137 a         2317 c         2279 g         1062 t
ORIGIN      
        1 gatctcgtcc caaattccga ggaacgtcgc cggcaccgag cgcggtgtgc ggccgatggg
       61 gagctgatcg acggccacgg cgcggctcag cggctcgaag ccgacgaggc gtgagtgcgt
      121 gcccggctct tctgctacga ggccgagctt ctgccgcagc gccggcaaga gcaccttgcc
      181 gacgagcgtg cttttaccgg agccgctcac gccgcagacg acgttgaagc gaccgagagg
      241 aatggagaga tcgacgttct tgagattgtg cgctgtcacc ccttcgaggc gaaggcgcgg
      301 cgtgctctcg ttgatggcga gcggtgcgcg cagctcggcg cgctccgata gcgcgcgacc
      361 cgtgggcgat tcgggatttt cgagtacgac ctcgggggcg cccgccgcga ccagccgacc
      421 accgcgcgag ccgcctgcag ggcccatgtc gacgaggtag ttcgcggccc ggatcgtctc
      481 ggcgtcgtgt tcgacgacca gcacggtgga gccgagctcg accaagcgct ccaaatttcg
      541 aatcaggcgc gaggtgtcgc gcgggtgcaa gccaatggtc ggctcgtcga gcacgtagag
      601 cgcgcccgtg aggcccgcgc cgagctgcgc cgcgagccga aggcgctgca tctcgcctcc
      661 ggagagggag tgtgctgcac gatcgagcga gaggtagtcg aggcctacgt cgaccaaaaa
      721 cgcgaggcgc cgttcgagct ctttcaacaa aggcccaccg atgcgctcct cggccccgct
      781 gaacgagagc gaccgcacgc gctcgagcgc ggccccgacc gcgcgcgccg aaaactcgtg
      841 gtaacgctcg ttttgcacgc gcactccacg cggaatcgga gcgaggcgtg agccgccgca
      901 gtcggggcac gtggacgtga cggtgcgcgt cactttgttc ttgccgcgcc ccgtgacgcg
      961 ctcttcgacg agcacgccgt cgccctcgca gcgctcgcag cggccctgct tggttgcgaa
     1021 cgagaaccag cgtgggtcga gctccggaag tgaaaacgcg cagacggggc acgccgaagt
     1081 cgtcgagagc agctgctctt tgcccgactc ggagcgcagc ttgagcgcac ccttgcccca
     1141 gcgtagcgcc gcgttcagcg acgcctggct gaagcgcgcc gcgtcaccgg gcggttcgat
     1201 gatcaagtcg atggtgtgct cgcgattgcg aacgaggcgc ggcggattgt cggttttgac
     1261 gagctcgccg tcggcgaatg cgtgctcgat gccggcgcgc gcagcggcgc tgaaaatgtc
     1321 gaggtacaca ccttttctcg cctgcacgac cggtgcgagc agatacggct tacctttcac
     1381 tgcgcgaacc gcctcgacca gcgcgtcgtt cgacataccg ccgatgggca aatcgtgcgt
     1441 tgggcaatgc aacgtgccga gcttcgcgta caagagccgc agaaaatggg cgacctcggt
     1501 gacagtcgcg accgtcgact ttccgcccgc gcgcgccgta cgctgctcga gcgcgatgga
     1561 aggcggaacg ccggtcaccg cgtcgacatc cgggcgcggc atggtcggca ggaattggcg
     1621 cgcgtagggc gtcagcgtct cgaggaagcg gcgctgcccc tctgcgaaca cgacatcgaa
     1681 cgcgagcgtg ctctttcccg agccactcgg ccctgtcacg acggtgagcg cggcgtgcgg
     1741 aatttgcacc gagacgtcgc gcaggttgtg ctcgcgcgcc gactctacgc tgagcaccgc
     1801 gcgcgtggct ttgcgttgcc gtccgctcgg cggcggcgct ttgacgaccg ccttcgtggc
     1861 caccggctcg aagcgcgacg cgagcgcgcg accggtgcgc gtatcctttt tctgcagctg
     1921 ttcgggggtg cccgtcgcga cgacgctgcc gccgccttcg cccgcatcgg ggcccatgtc
     1981 gatcacgaaa tcggcgcgcg aaacgacgtc gaggtcgtgc tccacgacga gcacgctgcc
     2041 gcccgcgtcg acgattcggt ccagcgcttc gaccaggcgc tcgacttcgt ccacgtggag
     2101 accagcgctc ggctcgtcca aaacgtagag cgcgccctgg tgcttctcac cgagcgcgcg
     2161 agagagcttc aagcgctgcg cttcgccgcc ggagagcgtc gttagcggct gacccaagcg
     2221 caagtagccg agccccaaca tcgagagcgg accgagcgcg cgcgccaccg ccacttcgct
     2281 cgcaaacacc tcgagcgcgc ggtcgacggt catgtcgagc acgtccgcga tggtgacgcc
     2341 gttgcgctcg atcgccaata tttcgtcttt gaagcgcttg cctttgcaca cggggcaagt
     2401 gagccgcacg tccgcgagga actgcatttc caccgtctcg gcgccttccc ccgagcacgc
     2461 ttcgcagcgc ccgccctcga cgttgaacga gaagtcgccg gcagaaaagc cccgcgcctg
     2521 cgcttccggc tgcttcgcgt agagcgcgcg gatcgcgtcc caggccttgg tgtaagtcgc
     2581 ggcgttgccg cgcgacgtac gaccgagcgg cgcctgatcg accagcgtaa tgctcttgat
     2641 gtgctcggca ccatcgatgc gggtgtgcgc gcccggctgc tccagatcga gctcgcccaa
     2701 acgacgcccg agcgcgcggt gcacgatgtc gaccgcgagc gtgctcttgc cggagccgct
     2761 cgggccggtg agcgcgcaca cgacgccgag cgggatcttc acgctgacgt ctttgaggtt
     2821 gttttcgctc gcgcccacca cttcgagaaa gcctcgcggc ttgcgcgccg ggcgcggtcg
     2881 tggcgcgagg ccagctagcg cgcgcgcggt cgcgcctttg gttttgcgca acgcagaagg
     2941 cgccgcgtcg tgcacgactt tgccgccctg gctgccggcg ccgggcccga gctcgatgac
     3001 gcgatccgca gccgcgatca gggccggatc gtgctccacc acgaggacgc tgttgttgcg
     3061 cgcggcgagc tcgcgcagca gcgtcgtgag cggctcgacg tcgctcggat gcagacccac
     3121 cgtcggctcg tcgagcacga agagcgcgtt gttgagcgac gtcccgagcg cggcggtgag
     3181 cgtgacacgc tgagcctcac cgcccgaaag cgtgcgcgcc tgacgatcca gagtcaggta
     3241 gccgagcccg acgcgctcga ggtaggtgat gcgcgactcg agctcgcggc gaacgagctc
     3301 accttggggc gtgccgggct tgagctccga gagccagcgc cgcgcggtgc gcacctccat
     3361 ctcgtgcagg tcggcgatgc tcttgccgcg cacgtgatac gagagcgcgg tcgggttcag
     3421 gcgtcgcccg tgacaggcgt ggcagaggtc gtacgagcga tagcgcgaga gcaagacgcg
     3481 cacgtgcatc ttgtaggtgc gcgtctcgag ccagcggaac caacccgcga cgcccgggaa
     3541 cttgccgtcg tcgtagctgc cctccccttc gatgatgagc ttttgctgct tcgccgtcag
     3601 cgagcggaac ggctccgtca tcgggatctt gtggcgcgcg cagaatttct tcagctcgga
     3661 gcgttcccac gtcgtcgagg cgccgcgcca gggccgaatc gcgcctttcg cgaggctccg
     3721 ctcgggatcg ggaatgacct tcgaaaagtc gacccccaac gtgcgaccga aaccgcggca
     3781 ttcggggcac gcgccgagcg gcgactcgta agaaaagagg cccggtcgcg cttcgggcag
     3841 cgagcgtcca cattcggggc agccgaggcc gcgccgcaag gtgagctcgc gctgctccga
     3901 acgcaccacg catacgccgc tgccgtgcgt ccacgccgtc tcgatgcctt gcgagatgcg
     3961 cacctcgtcg tgcccgttca cgcgatcgag aacgaccgcg agcgaatctt tcgcggtgac
     4021 ggcgcgcgaa ggagccagct ggtcgatgtc gacgagcgcc ccttcgaccc acatgcgacg
     4081 aaagccctgc cgcgtgagcc cgtcgcgcac ttccaagtac gactcggcgc tctgcaccgc
     4141 gagcggatac gtcacgatcg cgcggtcgcg cccgaggaca cgcgtcacgc gggccgccgc
     4201 gctcttcacg tcgagctctt cggctgccac gccgtgctcg gggcagaccg gccgcgcttc
     4261 gcgcaagaac agcgccgcga ggtagggctc gaggtccgcc atggtcgcga gagtcgagcg
     4321 cgagctcttg atcggggcgc ggcgatcgac cgcgacgccg gccggcactg gctcgaggcg
     4381 ctggacgggc gggcgctcga gccgctccaa gaattgccgc gcgtagggac tgaagctctc
     4441 gacgaagcgg cgctggccct ccgcgtacag cgtgtccatc gccaagctgc tcttgccggc
     4501 gccggacacg ccggtgagaa cgatgagagt tccgggctcg atctcgagcg agacgtggtc
     4561 gaggttgtgg gtgcaagcgc cttcgaggcg agtcggcagc attcggaagc accttctctg
     4621 cggccgcacc gctggttagg caagccggcg ccccgcgtgc cgtagcaccg tacacagctc
     4681 ggctcgccgt tcggcgtcat ttgcgccgct ttcgccccgc gcgtggcgag agcgccgcgc
     4741 gagtagtaga aactcgcccc ccatggcccg cgccaaagcc gccgctggag gaggcgatcg
     4801 tctggtttcg aagaaccggc gcgccttctt cgactacgag atcagcgaca ccttcgaagc
     4861 ggggctcgtc ctcatcggga gtgaggcgcg ctcgctacgc gagaactcga ccgacctcac
     4921 cgacgcctgg atcgacatcg atggccgagg cgaagcctgg gtgaagggca tgcgcatccc
     4981 cccgttgaag cacgctgcct tcggtcacgc cgacgtgcga ccgcgaaagc tcttgctgca
     5041 ccgcgggcag atcgatcgca tgcgcggcct caccgagcgc gacggcatga cgctcatcgc
     5101 gaccaagtgt tacatcaaga acaaccacgc aaagctcgag gtcgcggtcg ccaagggcaa
     5161 gaagcaacac gacaagcgac agagcatcaa ggacaaggaa atggatcgcg aagcccgcgc
     5221 cgccattcgc cgaggtttgc gatgagccgc gaggtcccgt tggttcgcgg cggagtgggc
     5281 cacatcgaaa gcgccgacgc gcagcacgtc gtggtagaca tcggggtgcc gtcgcccccc
     5341 ggctcgacgg tggagctctt ggtggcgaat gctccactcg gcgtcaaggt gcgcagctgc
     5401 cgccgtcttc cggatgtcga accgacacgg tttcgggtcg aagggcgctg gatcagcctg
     5461 agccgcgttc agcgcgaagc gctcggcatc gagctctaga ccgctgcctt ccgttagcct
     5521 cggcgaattc gtgaccgtag gtctcgctaa tcctgctacc ttttctcatc gatggtcgtg
     5581 cgcggtcggg ctctcgggtt ggcgtcagcg gcgctcgctt tttcgacgat gctgggcgcg
     5641 agttctgcct cggcgttctg tcgctcgcgt gcctgcgagt tcgacaaacc agcgagcgtc
     5701 ccgtgtaagc gggactcctc tggctgcccg acgaacggcg cggagctcta ctggcccgcg
     5761 ccgagcttcg acatgtgggt cgaccacaag ggctcaccca agtacggcat cagcggagag
     5821 cagctgctcg acctcacgca agaagcgctc gggaagtgga cgaacgtcga ctgcagcagc
     5881 gcgtcaccga gcatcgcagt cgccaaagtc gagctcatgc aagacgacgc cgccgtcgcg
     5941 gcagggctcg ttcaaccaca gaaagcgacg tccaccgacc cagccgtaac taccagcgtg
     6001 gtcgccttca tcgacgaagg ctggatgaaa gagagcgcgg acgccatcgc tctcaccacc
     6061 gcgagcttcg gcacacagag cggtcgtatc ttcggtgccg acatcgagct cgattcgacc
     6121 gactggcgct tcacgctcga cggcgatccc tcacctcaat acgacctctt gtcggtgctc
     6181 acgcacgaaa cagggcacgt ctttggtctc gccgatttga ggaccccggg cccgacgatg
     6241 tacggcagct acggggaggt cggcaatacg gacaagcgct cactcgaaac cgacgacgag
     6301 caggggatgt gcgcgatcta cccgccgggg cgcttcgaca aagacggcgg ttgcggctgt
     6361 cgtttggaag gccgcacgcg accgcatccg ggaacttggg tcggagttgg cctcgccgcg
     6421 ctcgggctcg tgctgcgacg ccgacgagca cgccgcggcg atcaaggcgt caacacgacg
     6481 cccaacgctc cccccaaata aaatccgccc aagctctcgc gccgcacatc cagatgctcg
     6541 atcggcaccg ggcgcaacgt gaaccccgct tcggcgccga gcgataggcg tgcccaacgc
     6601 gcgagccgcg gctgcaatcg caccgcgacg ccagcgcgcg cactccagct atcgcgctgc
     6661 gattcaatct cgtccacggc gcgcactccg ctcacgtgca cgctcgccac cgcaaccagc
     6721 gcggccagat cgacatccag ccgcggcccc ggatgcaagc ggagggctgg gccgacgtcg
     6781 agctcgagcc aggcg
//
LOCUS       DASTKS010001192         6454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1308032, whole genome shotgun sequence.
ACCESSION   DASTKS010001192 DASTKS010000000
VERSION     DASTKS010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308032"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFQ35_25385"
     CDS             complement(<1..712)
                     /locus_tag="VFQ35_25385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934071.1"
                     /translation="MGFRVLAAALVGAASLASATPSSAAWLETRVKSHITQIDVEARG
                     TATVTQELVLSVRGGPLKTFEIAGADADAEMLPDASVVPIVKYGVPAPIPLTIERQDD
                     GTLRIEIEREKGLFTGSYTFRFGYRTALLARDRIRRRGTAAEVEWIGPRFSDGIDVAK
                     VIFRLPEGSVTPALAQGNDADDAALGSAFLSSVRHVGGKVELEVVRPHVARGEPPLWR
                     LVTEPKIFEGLPPKANDVP"
     gene            complement(866..2812)
                     /locus_tag="VFQ35_25390"
     CDS             complement(866..2812)
                     /locus_tag="VFQ35_25390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934072.1"
                     /translation="MRSRAIALSVLAGFGCASKPPSAAPLAQAPTASAPLQAESPVDL
                     SPVAAPDDLIAIGRLARPRAFVETLASWSGFPLHLADVLPSEAQVLNQVVSWDAPVEA
                     VAVIDRHSSEKVAPPEYVVSVGLLSVDSALRAAKDKGFEVTRVSTQVYRVPLAEDVSC
                     AVAAAAGSAPARLVCSEKWQSVEDLLPYATRGLPREDFGGRDLYLALKPAPLQARYGQ
                     EISSLPLFVGIGLRQVQTDSPRLDRALADAAYGIAGELKTLALSLNGFDISARIDDRA
                     KSLDFGYSFGFAKDESFTAQLLQDSGHRSSVPNETFWDLPRTASSGQFTTGFDPKRLA
                     ALFGPLGEITDAFLEQRKAPPTYRSRFRSVFDSLPIAFSGAARVSGSTPPAKELTAQS
                     LFASNLGWHVGVQEVRADRLYKLMTDLEALLADRETPKLIKELLPKENPALPKVRHKV
                     VKVAGFPARGTAFIGEIPYSLVEKMVEKSALAASVKDAKKDAKKPVSVVVVLVPDGER
                     TYYSIAADERSAISVLEGARAGRDGKLSENPELARLKERPSMGVGFSSLEALFGPFSA
                     LARAVKFDAQGALSLAPNHGRSAWITRVEHLPLQNGGLRVSATISVPQGAFQDVAGIA
                     PALISAFASHGAGKSDSSRSATIR"
     gene            complement(3097..5016)
                     /gene="dnaK"
                     /locus_tag="VFQ35_25395"
     CDS             complement(3097..5016)
                     /gene="dnaK"
                     /locus_tag="VFQ35_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9934073.1"
                     /translation="MAKIIGIDLGTTNSVVAVMEGKEPKVIVNEEGSRLTPSVVAWDD
                     KGEVLVGQIAKRQAVTNPENTVYSAKRFIGRRFEEGEKEASRVPYKVVKGKNGDAWIE
                     VRGKAMAPPEVSAKVLLKLKKAAEDYLGEKVTEAVITVPAYFNDAQRQATKDAGKIAG
                     LEVRRIINEPTAAALAYGLDKKKNEVIAVYDFGGGTFDISILEVGDNVVQVISTNGDT
                     HLGGDDVDHLVMEWLAGEFRKDTGIDVTKDKMVIQRLKDAAEQAKIELSTKQETTINL
                     PFLTADASGPKHLSKQLTRAKLEQMIRPLVDRSMEPVRRALDDAKKKPSDIDEVVLVG
                     GSTRIPLVQETVQKFFGKEPHRGVNPDEVVAIGAAVQGGVLAGDVQDVVLLDVTPLSL
                     GVETLGGVMTTMIPRNTTIPTQKKEIYSTASDNQTSVEIHVLQGERAEARYNRTLGKF
                     HLEGIPPAPRGMPKIEVTFDIDANGILSVAAKDLASGKDQRITITASSGINQDEIERM
                     VKDAAAHEAEDKRRREEIERRNKLDSMCYTLERQIADNKEKLQGYDVSSIENLIKEGR
                     SAIEKQDDAKVQDVLGRLEKEAHAMAAKLYESVGGAGGAPGGAPGGAAPGAGANEPGA
                     GKKGDVIDAEFEETN"
     gene            5400..5472
                     /locus_tag="VFQ35_25400"
     tRNA            5400..5472
                     /locus_tag="VFQ35_25400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5433..5435,aa:Ala,seq:cgc)
     gene            5728..>6454
                     /locus_tag="VFQ35_25405"
     CDS             5728..>6454
                     /locus_tag="VFQ35_25405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934074.1"
                     /translation="MGGVKGGSNVGGGPAGGTSGAAGAGGEGVADSDDANRWATGATL
                     YVDDDPGCEGFVPCYRTLEDARSAAKHGDTILVLPGSYAGMRFGQYKNLGSLRILGRD
                     GPATTTITGPCFKIFSEAHPPGEIWVEGFTFRDCGPATASSADGWGLYISAQSAADVQ
                     VRNNVFGDQNGSGGLDLYSLGNARVHVVVTGNRFLNERKRDDNWGQFWAALFLDLSGS
                     SERDDDVYVRVENNLFAGNAVGAS"
BASE COUNT         1066 a         2100 c         2087 g         1201 t
ORIGIN      
        1 ccggaacgtc gttcgccttc ggtggcaggc cttcgaagat cttcggctcg gtcacgaggc
       61 gccaaagcgg gggctcgccg cgagcgacgt gcgggcgcac gacctcgagc tccacctttc
      121 cgccaacgtg ccgcaccgac gagagaaagg cactaccgag cgcggcgtcg tcggcgtcat
      181 tgccctgcgc cagagccggt gtgacgctgc cctctggaag ccggaagatc accttcgcga
      241 cgtcgatgcc gtcgctgaag cgaggtccga tccattcgac ctctgccgcc gtgccgcggc
      301 gccgaatgcg atcgcgcgcg agcaacgccg tgcggtatcc gaaccggaac gtgtagctcc
      361 cggtgaaaag ccccttctcg cgctcgatct cgatgcgcag cgtgccgtca tcctggcgct
      421 cgatggtgag cgggatgggc gcgggaactc cgtactttac gatcgggacg acgctcgcat
      481 ccggaagcat ctccgcgtcc gcgtccgcac cggcgatctc gaacgtcttc agcggccccc
      541 ctcgcacgga gagcacgagc tcttgcgtca ccgtcgcggt gccgcgagct tcgacgtcga
      601 tttgagtgat gtgggatttg acgcgcgttt cgagccacgc ggcgctcgag ggggtagcgc
      661 tggcgagcga ggcagcgccc acgagggccg cagcgagtac gcgaaaaccc atgtaggccg
      721 catacctaca tggcgaagtt gaatcagggc aagaaaccgg cggctgctca gccccgaagc
      781 caacccagtc ttttccggcc gcggtctgca gcgagaacta agcggctcca atagagagag
      841 ctaagtcggc tctgggaccg acagtctacc tgatggtggc ggaccgcgaa gagtcggatt
      901 tgcccgcgcc gtgggatgcg aacgcggaaa tgagcgcggg cgcgatgccc gcgacgtctt
      961 gaaacgcgcc ctgcggaacg ctgatggtgg cgctcacgcg caagccgcca ttttggagtg
     1021 gtaagtgctc cacgcgcgtg atccacgccg agcggccgtg gttcggcgcg agcgacaacg
     1081 caccctgcgc gtcgaacttg actgctctcg cgagtgcact gaagggcccg aacagcgcct
     1141 ccagactcga gaagccgacg cccatggatg gacgctcctt caagcgcgca agctcggggt
     1201 tttcggagag cttgccgtcg cggcccgcgc gcgcgccttc gagcaccgag atcgcgctgc
     1261 gttcgtccgc tgcaatcgag taataggttc gctcgccgtc gggaacgagc accacgacga
     1321 cgctcaccgg cttcttcgcg tccttcttcg cgtctttgac gctcgcggcg agcgcgctct
     1381 tttcgaccat cttctcgacg agcgagtacg gaatctcacc gatgaatgcg gttccgcgcg
     1441 ccgggaaccc ggcgaccttg acgactttgt gccgcacctt cggcagcgcg gggttttcct
     1501 tgggcagcag ctccttgatg agcttcggcg tctcccgatc cgcgagcagc gcctcgagat
     1561 cggtcatcag cttgtagagc ctgtcggcgc gaacttcctg aacgccgacg tgccagccca
     1621 agttgctcgc gaagaggctc tgggcggtga gctctttggc tggcggagtc gatcccgata
     1681 cgcgagccgc gccgctgaac gcgatcggta acgagtcgaa cacgcttcga aagcggctgc
     1741 gatacgtcgg cggcgctttg cgctgctcca gaaatgcgtc ggtgatttcg ccgaggggcc
     1801 cgaatagcgc agccagtcgc ttggggtcga acccggtggt gaactgcccg gagctcgcgg
     1861 tgcgcggcaa gtcccagaag gtttcgttcg gtacgctcga acggtgcccg ctgtcttgca
     1921 agagctgtgc ggtgaaagac tcgtctttcg caaagccgaa cgagtacccg aagtcgagcg
     1981 acttcgcgcg atcgtcgatg cgcgccgaaa tgtcgaagcc attgaggctt agcgcgagcg
     2041 ttttcaattc accagcgatg ccgtaggccg cgtcggcgag cgcgcggtcg agccgaggcg
     2101 agtcggtttg aacctgccgg agcccgatgc cgacgaagag cggcagcgag gagatctctt
     2161 gaccgtagcg cgcctgaagc ggcgccggtt tgagcgcgag gtagagatcg cgcccgccaa
     2221 agtcctctcg aggcaggccg cgcgtagcgt acggcagcag gtcctcgacg ctctgccatt
     2281 tctcgctgca gacgaggcgc gcgggcgcgc ttccggctgc agcagccacc gcgcaagaaa
     2341 cgtcctcggc gagcggtact cggtacacct gcgtcgagac gcgcgtcacc tcgaagccct
     2401 tgtccttggc cgcgcgaagc gccgagtcga cggagagcag ccccaccgag accacgtact
     2461 ctggaggcgc caccttctcg ctcgagtggc ggtcgatgac cgctaccgcc tcgaccggtg
     2521 catcccacga gacgacttga tttaggacct gggcctcgct cggcaacacg tccgctaagt
     2581 gaagcgggaa gccggaccag ctcgccaacg tctcgacgaa ggcccgcggc ctcgcgagcc
     2641 ggccgatcgc gatgaggtcg tcgggggcag caaccggaga caagtcgacc ggggactccg
     2701 cctgaagcgg agcgctcgcg gtcggcgcct gcgccaacgg tgccgcagac ggaggtttcg
     2761 aagcacaccc aaagccagcc aacacggaca gcgcaatcgc acgagatcgc attttttccg
     2821 cgactctaat gctccgctgc ggaagtcaca atcgttagtt ccgcgcttcg gtggcgcgga
     2881 tatcccacct catggtcgag cggtcgctcc ccgtcctcgc gtcgccgaac cagccggctt
     2941 ccgcgccgtc gagtgcggag cgcgacgtca gggtcgagct tcgcgaatcc gatccccctg
     3001 tcgggacgca gggcgttttc aacgacccgc taaaaagcga acggcgggcc ttcgaaaaga
     3061 cccgccgctt tcgctcggcg ccgtaactcg attcgctcag ttcgtttctt cgaactcggc
     3121 gtcgatgacg tcgcccttct tcccggctcc aggctcgttc gcgccagcgc ccggtgccgc
     3181 tccacccgga gccccgcccg gtgcgccgcc ggcaccgccg actgactcgt agagcttggc
     3241 agccatggcg tgcgcctcct tttcgaggcg accgagcacg tcctgcacct tggcgtcgtc
     3301 ttgcttctcg atcgcggagc gaccctcttt gatgaggttc tcgatcgagc tgacgtcgta
     3361 accctggagc ttctccttgt tgtcggcgat ctgacgctcg agcgtgtaac acatgctgtc
     3421 gagcttgttg cggcgctcga tctcttcacg gcgacgcttg tcttccgctt cgtgggcggc
     3481 cgcgtccttc accatgcgct cgatctcgtc ctggttgatg ccgctcgaag cggtgatggt
     3541 gatgcgctgg tccttacctg aggcgagatc cttggcggcc accgacaaga tgccgttggc
     3601 atcgatgtcg aaggtcacct cgatcttcgg catgccgcgc ggagccggcg gaatgccctc
     3661 gaggtggaac ttgccgagcg tacgattgta gcgagcctcg gcgcgttcgc cctggaggac
     3721 gtgaatctcg acgctcgtct ggttgtcgct cgccgtcgag tagatctcct tcttctgcgt
     3781 cgggatcgtg gtgttgcgcg ggatcatcgt ggtcatcacg ccgcccagcg tctcgacacc
     3841 gagcgagagt ggagtgacgt cgaggagcac gacgtcttgc acgtcgcccg cgagcacgcc
     3901 gccttgcacc gctgctccga tcgcgacgac ctcgtccggg ttcacgccgc gatgaggctc
     3961 tttgccgaag aacttctgaa ccgtctcctg cacgagcggg atgcgggtcg agccgccgac
     4021 gagcacgacc tcgtcgatgt cgctcggctt cttcttggcg tcgtcgagcg cgcgccgcac
     4081 gggctccatg ctgcgatcga ccagcgggcg gatcatctgc tcgagcttcg cgcgggtgag
     4141 ctgcttcgaa aggtgcttcg ggccgctcgc gtccgcggtg aggaacggca ggttgatggt
     4201 ggtctcctgc ttcgtcgaga gctcgatctt tgcttgctcg gcggcgtcct tgagacgctg
     4261 gatgaccatc ttgtccttgg tgacgtcgat gccggtgtcc ttgcggaact ctcccgcgag
     4321 ccactccatg acgaggtggt ccacgtcgtc accgccgagg tgggtgtcgc cgttcgtcga
     4381 gatcacctgc acgacgttgt cgcccacctc gaggatcgag atgtcgaacg taccgccacc
     4441 gaagtcgtaa acggcgatga cttcgttctt cttcttgtcg agaccgtagg cgagcgccgc
     4501 tgccgtcggc tcgttgatga tgcgccggac ctcgagacct gcgatcttgc cggcgtcctt
     4561 ggtggcctga cgctgggcat cattgaagta cgccgggacg gtgatgaccg cctccgtcac
     4621 cttctcgccg aggtagtcct ccgccgcctt cttcagcttc agcagcacct tcgcgctcac
     4681 ctcgggcggc gccatggcct tgccgcgcac ctcgatccaa gcgtcgccgt tcttgccctt
     4741 gacgaccttg tagggcacgc gagacgcttc cttctcgccc tcttcgaagc ggcggccgat
     4801 gaaacgcttg gccgagtaaa cggtgttctc ggggttggtc accgcctgac gcttcgcgat
     4861 ctgcccgacc aaaacctcgc ccttgtcgtc ccaggcgaca acggaaggcg tcagacgtga
     4921 accttcctcg ttcacgatga ccttgggctc cttgccctcc attaccgcca cgacgctgtt
     4981 ggtcgtgccg agatcgattc cgatgatttt ggccattttt ccgtattctc cctaactcct
     5041 caccgctccc ggctgcttac gggctcggcg tcgcgcttcc ttcgaacgct cactaggagg
     5101 tttcgggggg caacataacc acgtcgaccc tggggtcaac cggccaagtg acacgtccgc
     5161 aaagctctgc gcgttggtcc gtcgccggcc tcgcgctcgg tctcgccgcg tcccgcgcac
     5221 ggtctatcgt gcgggcggac cagggggagc gaacgcagcg ggccatcgcg gcgaacgggg
     5281 agctcggacc gacgagaaac tgcggccaaa ctgcgggcca ccccaagaaa aatgagcgaa
     5341 gcgacctgca ggctattgac agggccgtag agaacgctac tttcccgccc ctcattcccg
     5401 gggccgtagc tcagctggga gagcgccgcg ttcgcaatgc ggaggtcagg ggttcgatcc
     5461 ccctcggctc cacaatataa gtacccgaag cctcgaggct cttcccgacg agggcgaggg
     5521 ggctcgcgta gtatgaaccg caagctgatt gcggtctggc acttcgcgga gatactcgtt
     5581 gcggggatgc cctccgcgcc tctgtccttg ctgcgcgcct tggtcttttg cttcgcggcc
     5641 gccctctctc caagttgcgg tcgtgcagaa catacacacg cgggcggggg agcggcagct
     5701 ggcgcaggcg caagcagcag ctctgacgtg ggcggtgtga agggtggctc caacgtgggc
     5761 ggcggaccag cgggaggtac ttcgggtgcc gcgggcgcgg gcggcgaagg cgtggcggac
     5821 agcgatgatg cgaaccgatg ggcgactggg gcgacgctgt acgtcgatga cgacccgggg
     5881 tgtgaaggct tcgtcccgtg ttatcgaacc ttggaggatg cgcgctccgc ggcaaagcac
     5941 ggagacacca tcctcgtgct gcccgggtcg tatgctggca tgaggttcgg gcaatacaag
     6001 aatctcggat cgctcagaat cctcggacga gacggccccg cgacaaccac catcacagga
     6061 ccgtgcttca agatattttc ggaagcacac cctcctggag aaatctgggt cgaaggtttc
     6121 acgtttcgcg actgcggacc ggcgacggcc tcatccgccg acggttgggg cttgtacatc
     6181 agcgcgcagt ctgccgccga tgtgcaggtc cgaaataacg tgttcgggga ccagaatggg
     6241 agcggaggcc tcgacctcta ctccttgggg aatgcccgtg tgcacgtggt tgtcaccggt
     6301 aatcgcttct tgaacgaacg gaagcgcgac gataactggg ggcagttctg ggccgcgctc
     6361 ttcctcgatc tgtcgggcag ctctgagcgg gacgacgacg tctacgtccg cgtggaaaac
     6421 aacctattcg ccggaaacgc cgtgggcgcc agcg
//
LOCUS       DASTKS010001193         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1532174, whole genome shotgun sequence.
ACCESSION   DASTKS010001193 DASTKS010000000
VERSION     DASTKS010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532174"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFQ35_25410"
     CDS             <1..189
                     /locus_tag="VFQ35_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934075.1"
                     /translation="PAQCTIVESLPRSMQLSFDHVYHFLDSHHVGVFEGIFEHVGVKG
                     RVRISMTSSSSGSFLLEW"
     gene            complement(242..457)
                     /locus_tag="VFQ35_25415"
     CDS             complement(242..457)
                     /locus_tag="VFQ35_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934076.1"
                     /translation="MNRHWKGIGAILLFACLAIVVAMGIGRPMVAVIGVVVAAVIGFV
                     LAIDWMRPRKPSNVPPNDGNDRFVPRT"
     gene            660..1292
                     /locus_tag="VFQ35_25420"
     CDS             660..1292
                     /locus_tag="VFQ35_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9934077.1"
                     /translation="MATLSAVEALTRLREGNARFVANVRSVEAMASVARRADPLKPQE
                     PVAVILGCSDSRAPAETIFDQGMGDLFVIRVAGNIVAPSQIGSVEFAAERFGTRLVVV
                     MGHTHCGAVSATIEAILDPDPPRSRNLLSITDRIMPAVSPLMQTELARDRANLMRAAT
                     RANVAAAADHLRHGSRSLEEARLRGGLVIVGAEYDIESGQVDFFDGVDAL"
     gene            complement(1318..2010)
                     /locus_tag="VFQ35_25425"
     CDS             complement(1318..2010)
                     /locus_tag="VFQ35_25425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9934078.1"
                     /translation="MVERGPPPPPTPSPSRVVHHGDGLAWLRATALASDHAIVTSLPD
                     SSEVPRLGFDGWRSWFIDTAELVCRSVADDAVAIFFQTDVKREGRWVDKGFLVALGAE
                     RASSHLLWHKVVCRAPVGTTTFGRPAYAHLMCFSRGLLLAPAQSSADVIPELGAMPWA
                     RAMGTRACLEACRFLLKHTACRKVVDPFCGMGTLLAVANHLGLDAIGVELSQKRARRA
                     LALALDPTTLDS"
     gene            2074..2703
                     /gene="lepB"
                     /locus_tag="VFQ35_25430"
     CDS             2074..2703
                     /gene="lepB"
                     /locus_tag="VFQ35_25430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9934079.1"
                     /translation="MIGQAAWFSPWMARARTLRSFLTPMLTMAAMLAARASLADHYTV
                     PSGSMEPTVQVGDHVCVDKLAYGLRLPVSHSYVVERDGPARGDVVVLTSPSDGEVLLK
                     RVAAIPGDEVSVHGGRLTINGAEVPVEERSEGLVEQLGAHRHELGVDYGGGPDLTTTR
                     VPARRYLVLGDNRGNSRDGRYFGWVERSAILGRASAVCLREGWPVWRPL"
     gene            complement(2817..>3212)
                     /locus_tag="VFQ35_25435"
     CDS             complement(2817..>3212)
                     /locus_tag="VFQ35_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934080.1"
                     /translation="ADCARSLACKQGSQCAAKEGRCTASEESCKKSSVCANEGKCGVS
                     EGKCAAVSAGDCRSSGRCKLEGACSLKAGACIAASSADCAQASVCTKAKRCQAKDGVC
                     IGSGGGKGGSEKSGPPDKVGSTTISGLGI"
BASE COUNT          530 a         1073 c         1046 g          563 t
ORIGIN      
        1 cccgcgcagt gtaccatcgt cgaaagcttg ccgcgctcca tgcagctgtc tttcgaccac
       61 gtctatcatt tcttggactc gcatcacgtc ggtgtgttcg agggcatctt cgagcacgtc
      121 ggcgtcaaag ggcgcgtgcg catctcgatg acgagctcga gctcgggcag tttcctgctc
      181 gagtggtagt ccgttcgctc gactgctccg ccgagcgaac ggagataatc ggtgtccgtg
      241 ctcacgtgcg gggcacgaat cgatcgttgc cgtcgttcgg cggcacgttg ctcggcttgc
      301 ggggtcgcat ccaatcgatg gcgagcacga atccgatcac ggctgcgacg accacgccga
      361 tgacggccac catgggccgc ccaatgccca tcgccacgac tatggcgagg caagcaaaga
      421 gcaggatcgc gccgatccct ttccagtgtc tattcatttg gcacctcggg ttgaacccga
      481 ctccagccgc tgcaatcgcg gtgccatcgc catttcgcct cgcctgacat ttctgtcgca
      541 gcgtgcgtgg gcttcgaagc tgccgtaccc gcgccgattc gaccccaaat ccagcaaccg
      601 gtgcagtttt cgcacggggc ctctcgacgc cgcacaaata gctaaggtag gagctcgcaa
      661 tggctaccct gtctgctgtc gaggccctta cccgcctgcg cgaaggaaac gcgcgattcg
      721 ttgccaacgt tcgcagcgtc gaagcaatgg cgagcgtcgc tcgacgagca gatccactga
      781 agccgcaaga gccggtcgcg gtcatcctcg gctgctcgga ctctcgcgcc cctgccgaaa
      841 ccatcttcga ccaaggcatg ggcgatctgt tcgtcattcg cgtcgcgggc aacatcgtcg
      901 cgccgtcgca aattggtagt gtcgaattcg cggcagagcg cttcggcacg cggctcgtgg
      961 tggtgatggg gcacacgcac tgtggcgcgg tatccgccac gatcgaagcg attttggatc
     1021 ctgacccgcc gcggtcgcgc aacctgctct ccatcaccga ccgcatcatg ccggcggttt
     1081 cgccgctgat gcagaccgag cttgcccgcg accgtgcgaa cctgatgcgc gccgcgactc
     1141 gagccaacgt cgccgccgcc gcagatcacc ttcgccacgg ctcgcgctcg ctcgaggagg
     1201 cgcgtcttcg cggcggtctc gtgatcgtag gcgccgagta cgacatcgag agcggccagg
     1261 tcgacttctt cgacggagtg gacgcgctct aggcggctcg ggccgtcgtt cgtagtgcta
     1321 cgaatcgaga gtggttgggt cgagcgcgag ggctagcgct cgacgcgctc tcttctgcga
     1381 gagttcgacg ccaatcgcgt cgaggccgag gtggttcgcg acggcgagta gtgtccccat
     1441 tccgcaaaac gggtcgacga ctttgcggca ggccgtgtgc ttcagcaaaa agcggcacgc
     1501 ttcgaggcag gcgcgcgttc ccatggcgcg agcccacggc atcgctccga gctccgggat
     1561 cacgtccgcc gaagactgag cgggagcgag cagcagccct cgagagaagc acatcaggtg
     1621 cgcgtaggca gggcgaccaa acgtcgtcgt gccgacgggc gcgcgacaaa cgaccttgtg
     1681 ccaaagcaga tggctcgacg cgcgctccgc gccgagggcg accaagaagc ctttgtcgac
     1741 ccagcgccct tcgcgtttga cgtccgtttg gaaaaagatc gccaccgcgt catcggcaac
     1801 gctgcggcac acgagctccg cggtatcgat gaaccagctg cgccagccat cgaagccgag
     1861 gcgcgggacc tctgacgagt ccgggagcga cgtgacgatg gcatggtcgc tcgcgagggc
     1921 ggtcgcgcgg agccaagcca gcccgtcgcc gtgatgaacc acacgagaag gggatggggt
     1981 gggtgggggc ggcgggccgc gttcgaccat gcgaggcgcg cagggtagcg cgaaaacggc
     2041 catgcccccg ctacggagct ctgctcgctg ggaatgatcg gccaagccgc gtggttctca
     2101 ccttggatgg cgcgcgctcg aacccttcga agctttctga ccccgatgct gacgatggcg
     2161 gccatgctgg ccgcgcgagc gtctctcgcc gaccactaca cggtcccgag tggctcgatg
     2221 gaacccacgg tgcaggttgg cgaccacgtg tgcgtcgaca agctcgctta cgggctccgt
     2281 ctcccggtat cgcactctta cgtcgtggag cgagacgggc cggcgcgagg cgacgtcgtc
     2341 gtcctcacct ctccgtcaga cggcgaagtc ctgttgaagc gggtggcggc gatccccggc
     2401 gacgaagtca gcgtgcacgg cggccgcctc accatcaacg gtgccgaagt tccggtcgaa
     2461 gagcgcagcg aggggctggt cgagcaactc ggcgcgcatc gacacgaact cggcgtcgat
     2521 tatggtggcg ggcccgacct cacgacgacg cgcgtgcctg cgcggcgcta cctggtgctg
     2581 ggagacaatc gcggaaacag ccgcgacggc cgctactttg gttgggtcga gcgcagcgcg
     2641 atcctagggc gagcgagcgc ggtgtgcctg cgcgaagggt ggcccgtgtg gcgcccgctg
     2701 tgacgcgaag gcgagcgccc gctcttcgcc cacgtcagtg caggcgcact cggcgccccc
     2761 gatgacgctc tgtcggcact ttttcgactc gaactcgcgc gtcggcagtg gtcccttcag
     2821 atgccgagcc cgctgatggt cgtcgaacca accttgtcgg gggggcccga cttctcgctg
     2881 ccacccttgc cgccgccgct cccgatgcac acaccgtcct tcgcttggca gcgcttggct
     2941 ttggtgcaca ccgacgcttg agcgcaatcg gccgagctcg cggcgataca agcgcccgcc
     3001 ttgaggctgc acgcgccttc gagcttgcat cgtccggatg aacgacaatc gccggcgctc
     3061 acggcggcgc acttcccttc gctcacgccg cactttcctt cgttggcgca cacgctcgac
     3121 ttcttgcagc tctcttcgga ggcggtgcac cgaccctcct tcgctgcgca ttgcgagcct
     3181 tgcttgcaag cgagcgagcg ggcgcaatcg gc
//
LOCUS       DASTKS010001194         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_636232, whole genome shotgun sequence.
ACCESSION   DASTKS010001194 DASTKS010000000
VERSION     DASTKS010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636232"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFQ35_25440"
     CDS             complement(<1..235)
                     /locus_tag="VFQ35_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET9934081.1"
                     /translation="MSQFATNTSLPRYLRVEHERIEKIARKVGLDFFPVVFEMLSYDQ
                     MNEIAAYGGFPNRYPHWRFGMEYERLSKSYEYGL"
     gene            complement(243..1469)
                     /locus_tag="VFQ35_25445"
     CDS             complement(243..1469)
                     /locus_tag="VFQ35_25445"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET9934082.1"
                     /translation="MFHRPAIRARERSASYLGSLAETEFAFERGEMCDTRYSMSLKID
                     QDHSRFRNIVRGKIRQNLRRYISQGEMLGRKGKDLVSIPIPHIDIPRFVFGDKQNGGV
                     GQGDGDVGDAVGGQEAEGQDGSGKAGKDAGDHVLEVDITLDELADILGEELQLPDIEN
                     KGKSRIIAAKDRYTGIRRVGPESLRHFKRTYREALKRAIASGVYNPALPVVVPQQEDK
                     RFRSWKEDLEPVANAVIIYMMDVSGSMGDEQKEIVRIESFWIDTWLRRQYQGLESRYI
                     IHDAVAREVDRDTFFRTRESGGTMISSAYRLAAQLIDQHYPSEEWNIYPFHFSDGDNW
                     SMEDTLACVELLKKEMLPKVNMFAYGQVESPYGSGQFIKDLREHFSSDERVLTSEIRD
                     RDAIVSSIKDFLGKGK"
     gene            1606..2205
                     /locus_tag="VFQ35_25450"
     CDS             1606..2205
                     /locus_tag="VFQ35_25450"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9934083.1"
                     /translation="MNKTHKLFKFRCTGCGNCCKDPLLPMTDADLRRITERTGDVAAD
                     VVRFVDRNAIDMDDEPEAFLSLKDGKRVMILRHQGGGCRYLGDDNRCTIYGARPLGCR
                     IFPFDPAYTKEGGLRRLKLIQATDCLYEMDGDNDPEGVRKLHGRYEAQNEAYLAKVAE
                     WNRLQAARKRAGRSVQTARQFLAFLGFEARPRVSRAPRA"
     gene            complement(2237..2773)
                     /locus_tag="VFQ35_25455"
     CDS             complement(2237..2773)
                     /locus_tag="VFQ35_25455"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9934084.1"
                     /translation="MSSEKGYLVVTAVGPDRAGIVSDLSSLIHAAAANLEDSRMAVLG
                     GEFALLLLVSGEKTLLERLAGELRAQEGSLGLHIHCKPTSGAKARANVLPYSIRVSGF
                     DRPGIVQSVTTILARRAINVTALESRVSHAPESGTPLFVLEADLEVPSEVALSELRRE
                     LSKKCDEENLDVTLEARG"
     gene            2808..3788
                     /gene="hemB"
                     /locus_tag="VFQ35_25460"
     CDS             2808..3788
                     /gene="hemB"
                     /locus_tag="VFQ35_25460"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET9934085.1"
                     /translation="MELTKRPRRNRRTAALRGLVRETTLGPGHLVLPLFVQEGNGLAT
                     PINAMPGQARLSIDLLLKTAEEAVSLGVNAVALFPVIPDALKDARGSESANSAGLLPR
                     AVRALKGALPGLCVITDVALDPYSSDGHDGVLIDGRIDNDVTLPILAKMAVVQAQAGA
                     DVVAPSDMMDGRVAAVRAALDEAGHSDTAICSYCVKYASGFYGPFRDALDSAPRSGDK
                     KTYQMDPANVREGLREIALDEAEGADILMVKPGLPYLDVIRFVRENSTLPVAAYHVSG
                     EYTMLKLAAERGYLDYERCLLESLVCMRRAGADMIVTYAALDAARALRSS"
     gene            complement(3780..>4688)
                     /locus_tag="VFQ35_25465"
     CDS             complement(3780..>4688)
                     /locus_tag="VFQ35_25465"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET9934086.1"
                     /translation="LGKFGGSELGFGSDLDVIFVFDPAYAPSREEALPYFAARAQRII
                     RLLTEPNAAGPGYELDTRLRPSGSQGLLVTSIGAFARYHGVEAPEAHVVGLHATSSGA
                     AWERQVLLRARACAGDRRLGERVVELAQAAAYERGAPPVEELLRLRQRMEVELGRERA
                     GRFDLKTGRGGLLDVEFCVQWLQMRYGADPSVRSTDTASALGALHDHGYLARPLYITL
                     REGYRFLRRLEQRLHVFAGTSVSIIDAQGAGLNEIARSLGFSDDPGRSAAEQLLSRYE
                     TVTASVREAYLAALGAASDAPPTSSA"
BASE COUNT          785 a         1504 c         1550 g          849 t
ORIGIN      
        1 acagcccgta ctcgtagctc ttgctgagtc gctcgtattc catcccgaag cgccagtgcg
       61 ggtagcggtt cgggaagccg ccgtaggcgg cgatttcgtt catttggtcg tagctcagca
      121 tctcgaacac gaccgggaag aagtcgaggc caaccttgcg cgcaattttc tcgatgcgct
      181 cgtgctccac gcggaggtag cgcggcaggg acgtgttggt tgcgaattgc gacatgttcc
      241 gactatttcc ccttgcccaa gaagtccttg atgctgctga cgatcgcgtc gcgatcgcga
      301 atctcgctcg tgaggacgcg ctcgtcgctc gagaaatgct cgcgaaggtc cttgatgaac
      361 tgcccggagc cgtaggggct ctcgacctgc ccgtaggcga acatgttcac cttcggcagc
      421 atctccttct tcaggagctc gacgcaggcg agcgtgtctt ccatcgacca gttgtcgccg
      481 tccgagaagt ggaaggggta aatgttccac tcttcgctcg ggtagtgctg atcgatgagc
      541 tgcgccgcca agcggtaagc gctcgagatc atcgtgcccc cggactcgcg ggttcgaaag
      601 aacgtatcgc ggtccacctc acgcgccacc gcgtcgtgga tgatgtagcg gctctcgaga
      661 ccctggtatt gacggcgtag ccaagtatcg atccagaagc tctcgatgcg gacgatctcc
      721 ttttgctcgt cacccatcga gcccgacaca tccatcatgt agatgattac ggcgttcgcg
      781 accggttcga gatcttcctt ccaagagcga aagcgcttgt cctcctgctg cggcacgacc
      841 accgggagcg cggggttgta aacaccgctc gcgattgctc gcttcagcgc ttcgcgatac
      901 gtgcgcttga agtggcgcag cgactccggt ccgacgcggc ggataccggt gtagcgatct
      961 ttggccgcga tgatgcggct ctttcccttg ttctcgatgt cggggagttg aagctcctca
     1021 cccaagatgt cggcgagctc gtcgagcgtg atgtcgactt cgagcacgtg atcgccggcg
     1081 tccttaccgg ctttgccgga tccatcttgt ccttcggctt cttgcccgcc caccgcatcg
     1141 ccaacgtcgc cgtcaccttg gcccacgccg ccgttctgct tgtcgccgaa cacgaagcgc
     1201 ggaatgtcga tgtgcgggat cgggatgctg acgaggtcct tcccctttcg acccagcatc
     1261 tctccctgcg agatgtagcg gcgcaggttt tgtcggatct tgccgcgaac gatgttgcgg
     1321 aagcgcgaat gatcctggtc gatcttcaag gacatcgaat accgagtgtc gcacatttct
     1381 cctcgttcaa aagcgaactc ggtctcggcg agggagccaa ggtaggatgc cgagcgctct
     1441 cgggctcgga tcgctgggcg atgaaacatt gcggccgcct cgcggtgcgc tcaccgggcg
     1501 gagccgaggc gagccctcgg ccgtggggcc cgtcatttgt tccgtcgcgc ccgaagcctt
     1561 cgtggctctt gctcccggga gggaatctac ggaacatccc ggcccgtgaa caagactcac
     1621 aagctcttca agttccgctg caccggctgc ggcaactgct gcaaagaccc gctgttaccg
     1681 atgaccgacg ccgacctgcg tcgcatcacc gagcgcacgg gcgacgtcgc ggccgacgtc
     1741 gtgcgcttcg tcgatcggaa cgccatcgac atggacgacg agcccgaggc gttcctctcg
     1801 ctcaaagacg gcaagcgggt gatgattttg cgccaccaag gcggcggctg ccgctacctc
     1861 ggcgatgaca accgctgcac gatttatggc gcgcgccccc tcggctgtcg catctttccc
     1921 ttcgatcccg cttacacgaa agagggcggg ctgcgtcgct tgaagctgat ccaggcgacc
     1981 gactgccttt acgagatgga tggcgacaac gaccctgagg gcgtccgcaa gctgcacggc
     2041 cgctacgagg cgcagaacga ggcgtatctc gccaaagtcg cggagtggaa tcggctacaa
     2101 gcggctcgga agcgcgcggg ccgcagcgtc caaaccgcga ggcaatttct cgcgtttcta
     2161 gggttcgagg cgcggccgcg ggtgagccga gcgcctcgag cctagccgtt cgggcagagc
     2221 tcgcggctcg ggcttgtcag ccgcgcgcct cgagcgtgac gtccaggttc tcttcgtcgc
     2281 actttttcga tagctcgcgg cggagctccg aaagcgctac ttccgagggc acctcgaggt
     2341 ccgcttcgag aacgaagagc ggtgtgccgc tttcgggggc gtgactgact cgtgattcga
     2401 gcgcggtcac gttgatggcg cgccgcgcga ggatggtcgt cacgctttgc acgatgcccg
     2461 gcctatcgaa gccggacacg cggatggaat acgggaggac gttcgcgcga gccttggcgc
     2521 cgctcgtcgg cttgcagtgg atgtggaggc cgagcgagcc ctcttgcgca cggagctctc
     2581 cggcgaggcg ctcgagtagc gtcttttcgc cggacaccaa caaaagcagg gcgaattcac
     2641 cgccgagcac cgccatgcgg ctgtcttcca aattcgctgc cgcggcgtgg atcaaactcg
     2701 ataggtcgct cacgatgccg gcgcggtccg ggcccacggc ggtaacgacc aagtagccct
     2761 tctcactcga catttggcct cccagaagtc atagcgtagt gcctacgatg gagcttacca
     2821 agcgcccgcg cagaaatcga cgaacggctg ctcttcgtgg cctcgtgcgc gagaccacgc
     2881 tcgggccggg gcacctggtt ttgccgttgt tcgtgcagga ggggaacggc ctcgcgacgc
     2941 cgatcaacgc catgccgggg caggcgcgct tgtcgatcga cctcttgctg aagacggcag
     3001 aagaggcggt gtcgctcggg gtgaacgcgg tcgcgttgtt cccggtgatc cccgacgcgc
     3061 tgaaggacgc gcgcggcagc gagagcgcaa actctgcggg cctcctgcct cgagccgtcc
     3121 gcgctctgaa gggcgctttg ccggggcttt gcgtgattac ggacgtcgcc ctcgacccgt
     3181 attcgtcgga tgggcacgac ggcgtcctta tcgatggtcg aatcgacaac gatgtcacgc
     3241 tccccatcct cgccaaaatg gcggtagtgc aggctcaggc gggtgctgac gtcgtggctc
     3301 cctccgacat gatggacggg cgcgtcgcgg cggtgcgcgc ggcgctcgac gaggccggcc
     3361 actccgacac ggcaatctgc agctactgcg tcaaatacgc ctcggggttc tacggtccgt
     3421 tccgcgacgc gctcgattcg gccccgcgct ccggcgacaa aaagacctat cagatggacc
     3481 cggcgaacgt gcgcgagggg ctgcgagaaa tcgccctcga cgaggccgag ggcgccgaca
     3541 tcctgatggt gaaaccgggc ctgccgtacc tcgacgtcat ccgcttcgtg cgcgaaaaca
     3601 gcacgctccc ggtggccgcg tatcacgtga gcggtgagta cacgatgctg aagctcgcgg
     3661 ccgagcgagg ctatttggac tacgagcgct gcctgctcga gagcctcgtt tgcatgcgcc
     3721 gtgcgggcgc cgacatgatc gtgacgtacg cggccctcga cgcggcgcgc gcgctgcgct
     3781 caagctgagc tcgtcggcgg cgcgtcactc gcggcgccga gcgcggcgag gtaagcctcg
     3841 cggacgctcg cggtgacggt ttcgtagcgt gagaggagct gctcggctgc gctgcgcccc
     3901 gggtcgtcgg aaaatccgag tgaccgcgcg atttcgttca gccccgcgcc ctgcgcgtcg
     3961 atgatggata cgctcgtccc ggcgaaaacg tgcagccgct gctcgaggcg tcgcaggaac
     4021 cgatagcctt cgcgcagcgt gatgtagagg gggcgcgcga ggtagccgtg gtcgtgcagc
     4081 gcgccgaggg cggacgcggt gtcggtgctc cgaacgctgg gatcggcgcc gtagcgcatt
     4141 tgtagccact gcacgcagaa ctcgacgtcg agtagcccgc cgcgcccggt cttcaaatcg
     4201 aagcgcccgg cgcgttcgcg cccgagctcc acctccatac gctgccgcaa gcgcagtagc
     4261 tcctcgacgg ggggagcgcc gcgctcgtag gcggcggcct gcgcgagctc gacgacgcgc
     4321 tctccgagcc tgcgatcgcc agcgcaagca cgcgcccgca ggagcacttg gcgctcccac
     4381 gccgcgcccg aggacgtagc gtgcaggccg acgacgtggg cttcgggcgc ttcgacgccg
     4441 tggtagcgcg cgaaggcacc aatcgaggtg acgagcaggc cttgagagcc ggacggacgc
     4501 aagcgcgtgt cgagctcgta gccggggccc gcggcgttcg gctccgtgag cagccgaatg
     4561 atgcgctgcg ctcgggccgc gaagtagggc agcgcttctt cgcgggacgg cgcgtaggcg
     4621 ggatcgaaga cgaagatgac gtccagatcg gagccgaaac cgagctcgga gccgccgaat
     4681 ttgccgag
//
LOCUS       DASTKS010001195         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_413431, whole genome shotgun sequence.
ACCESSION   DASTKS010001195 DASTKS010000000
VERSION     DASTKS010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413431"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFQ35_25470"
     CDS             complement(<1..788)
                     /locus_tag="VFQ35_25470"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9934087.1"
                     /translation="MEVCSLSGNEIFCLQLKGFFPSEIVVGNSVRSLGLAGAIGSGFR
                     TLAGGEVENITELISEGRHAAISRLVEEARRHRAHGVTGVTTELSTLAGYTEFLSQGT
                     GVTAANATLPFFTTSASGTELYCHLDAGYMPISFVMGNVAYALGIGRGIAGGLSTLAR
                     GEVHQYSEMYNRIRHLALFRLRREAAAAGANAVVDVRIDLRPFGPGIVELLMTGTAAR
                     HPALAADSTDPANVATSELTGPELFSLAQLGYAPVQLVMATSVYS"
     gene            complement(882..>2259)
                     /locus_tag="VFQ35_25475"
     CDS             complement(882..>2259)
                     /locus_tag="VFQ35_25475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9934088.1"
                     /translation="ELGCASGGNLVPMAESLAQSSCLGIDLSSRQIEDGNRFIAQTGL
                     RNVSLRQASILDINESDGEFDYIVCHGVYSWVPRHVQAAILELCSKRLSPNGIAYISY
                     NTLPGWGMLGVLRDMMRFHASHFAEPAKQIEQSRALLKVLAAAVPSEENPYGMLLNQV
                     SEKVQKSADWYIYHEHLEEDNDPVYFHQFNARAEAVGLQFLGEAELLTMFSNQLPENV
                     QAALRGVSGNIVRSEQYMDFFRNRTFRETLLCRGGAQLKRKIDAERIRPLSFASELAP
                     RDGVIDLKPGVEVTFSAPGRGSVGVRDPIFKAALGALREAWPRFVSTAELEGLARKRL
                     ELGPATTETTNAFVERLWNWFTHRLVQMSSVPAACVSLPSERPVGSVVARAQLAAGRA
                     HLTNLLHSVVRVGTFEARVLSLLDGSRDRRALGQALQGSEHAAEAAKVGRVDAALRQL
                     AAAALLKA"
BASE COUNT          406 a          744 c          720 g          389 t
ORIGIN      
        1 gagtacacgc tggtcgccat cacgagctgg accggcgcgt acccgagctg cgccaggctg
       61 aagagctcgg gcccggtgag ctcgctcgtc gcgacattcg ctggatccgt gctgtcggcc
      121 gcgagcgccg ggtggcgcgc ggccgtgccc gtcatcaaga gctcgacgat gcccggcccg
      181 aacggacgca gatcgatgcg cacgtcgacc acggcgttgg ctcctgccgc cgccgcctcg
      241 cggcgcaggc gaaacagcgc gaggtgccgg atacgattgt acatctccga gtactggtgc
      301 acctcgccgc gcgcgagcgt cgagaggcca ccagcgatgc ctcgaccaat gccgagcgca
      361 tacgcgacgt ttcccatcac gaagcttatc ggcatgtaac ccgcgtcgag gtgacaatag
      421 agctcggtcc ccgaggctga cgtggtgaag aacggtagcg tcgcgttggc ggcggtaacg
      481 ccggtgcctt gcgaaaggaa ttcggtgtat ccggcaagcg tggagagttc ggtcgtaacc
      541 ccggtcacgc cgtgagcgcg gtggcgccgt gcctcctcga cgagccgaga aatggcagcg
      601 tgacgcccct cggagatgag ctcggtgatg ttctcgacct cacccccggc tagcgttcga
      661 aagcccgatc caatcgcccc ggcgagaccc aagctgcgca cgctgttccc gaccacgatt
      721 tcgctcggga agaagccctt gagctggagg cagaagattt cgtttccgga gagagagcag
      781 acttccacgc ccgcgacttt ataccggccg tcgtgcgggc gctctggttt cagctcacgg
      841 tcacgcgaag gtgtctctcg cgcgcgaagc ctcgggtttc atcacgcttt gagcagcgcg
      901 gccgccgcga gctgcctcaa ggcggcatcc acccgtccga cttttgcggc ctccgcagcg
      961 tgctccgagc cttggagcgc ctggccgaga gctcggcggt cgcggctgcc gtccaagagc
     1021 gaaagcaccc gagcttcgaa cgtgccgacg cgcacgaccg aatgcagcag attcgtgagg
     1081 tgggctcgcc ctgccgcgag ctgcgcgcgc gcgaccacgc tcccgacggg gcgctccgaa
     1141 ggaaggctca cgcaggctgc gggcacggag ctcatctgca cgaggcggtg agtaaaccaa
     1201 ttccacaaac gctcgacgaa cgcattggtg gtctccgtcg tcgccggccc gagctcgagt
     1261 cgtttgcgag ccaggccctc gagctctgcg gtcgagacga aacgcggcca ggcctcgcgc
     1321 agcgcgccga gcgccgcttt gaagatcggg tctcgcacgc cgacgctccc gcgaccgggc
     1381 gcgctgaacg taacctccac cccgggcttc agatcgatga caccgtcgcg aggcgcaagc
     1441 tccgaagcga acgacagcgg tcggattcgc tcggcgtcga tcttccgctt gagttgcgca
     1501 ccgccacgac agagaagcgt ctcgcgaaag gtacggttcc gaaagaagtc catgtactgt
     1561 tcggaccgaa cgatgttccc ggacacgccg cgcaaggccg cttgcacgtt ctccggcagc
     1621 tggttcgaga acatcgtgag gagctcggct tcacccagga attggagacc gactgcctcg
     1681 gcgcgcgcgt tgaactgatg gaagtagaca gggtcgttat cttcctcgag gtgctcgtga
     1741 tagatgtacc aatccgcgct cttctgcacc ttttcgctga cctgattcag cagcattcca
     1801 tagggatttt cttcgctcgg caccgcggcc gcgagcactt tgagcagcgc tcgggactgc
     1861 tcgatctgct tcgcgggctc cgcgaagtga ctcgcgtgaa agcgcatcat gtcacgcagc
     1921 acgccgagca tgccccaacc gggcagggtg ttatagctga tgtaagcgat cccgttcggg
     1981 ctcagccgct tactgcaaag ctcgaggatc gccgcctgaa catgtcgcgg cacccaagag
     2041 tacacgccgt ggcaaacgat gtaatcgaac tcgccgtcgc tctcgttgat gtccaagatc
     2101 gaagcttgtc gcaagctcac gttccgaagc ccggtctgcg cgatgaagcg gttaccgtcc
     2161 tcgatctggc ggctcgacag gtcgatgccc aggcatgagc tctgggccag tgactccgcc
     2221 atcggcacca agttgccgcc gcttgcgcag ccgagctcg
//
LOCUS       DASTKS010001196         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1084417, whole genome shotgun sequence.
ACCESSION   DASTKS010001196 DASTKS010000000
VERSION     DASTKS010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084417"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..566
                     /locus_tag="VFQ35_25480"
     CDS             129..566
                     /locus_tag="VFQ35_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934089.1"
                     /translation="MGLVVSMWLIDERRGFLDRLAEGFTRRTGLAAQVLPADVAQSQP
                     GGAPTGDPEAERFRVPYLREGFWIGPKPTYEGAFVAEWDGFPPHPYCYFHVNAASLEA
                     GARPLEPVPTVSERDTERYGARWSELPLLRRVLWRPGMFGLLR"
     gene            complement(571..987)
                     /locus_tag="VFQ35_25485"
     CDS             complement(571..987)
                     /locus_tag="VFQ35_25485"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9934090.1"
                     /translation="MPPIRQWKASVAKTYWMASAGVFLASLALVLVGFGVTGWLSLRL
                     WRRRSQLSNANKVIAVLATGLGLVGPIGTCLGLVKAFGAVGGESVDPSQRATILAEGI
                     AEAMNWTAFALVVWMASGVAFLVATVLARSARNKHP"
     gene            1212..1766
                     /locus_tag="VFQ35_25490"
     CDS             1212..1766
                     /locus_tag="VFQ35_25490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9934091.1"
                     /translation="MSLVGTDDVAGPDWVLAALRQHEQPLLRYAASLVGESRAQDVVQ
                     DAFLRLCSQSFESVADHLAAWLFTVCRNRALELRRAERRLSSMDDGDEDDARESPDSG
                     PIEKVERAENVSRVRAAMAALPRRHREVLRLKLEADLSYKDIAAVMNLSVGNVGFILH
                     TAIAKIREQLSEPLEAARAEGSAL"
     gene            1763..>2090
                     /locus_tag="VFQ35_25495"
     CDS             1763..>2090
                     /locus_tag="VFQ35_25495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934092.1"
                     /translation="MSIEKSDPRLTAYALDELSAEERRAFEAELEQSEGAQHELVEIE
                     RTLHELRFEFLDADPVKLESRRRVEIEAAAQTKVRAGRTSRRRKIWGGVSLGAVAASV
                     LLLTMKH"
BASE COUNT          377 a          653 c          698 g          362 t
ORIGIN      
        1 ctcaggtctc cgtgattccg aacctctcgt tgaaccacgc aggcgccacc gccatcgttc
       61 gcttctgaag ccggtcgcgc gatctgatta tccgaacgac agcggcaatc tgacgaacga
      121 acgttgacat gggcttggtc gtctcgatgt ggctcatcga tgagcggcgc ggcttcctcg
      181 accgccttgc cgaggggttc acgcgccgca ccgggttggc ggctcaggtg ctaccggctg
      241 acgttgcgca gtctcagcca ggaggcgccc ccaccggtga tccggaggcc gagcgctttc
      301 gggtgcccta tttgcgcgag gggttttgga tcgggccgaa gccgacgtac gaaggcgcat
      361 tcgtagccga atgggatggt tttccgccgc acccgtattg ctactttcac gtgaacgcag
      421 catcgctcga ggcgggcgcg cgtcccctcg agccggtgcc cacggtgagc gaacgcgaca
      481 cagaacgata cggcgcgcgt tggagcgagt tgccgctgct tcgtcgagtg ctgtggcggc
      541 caggcatgtt cggcctcctt cgctgacgag ctacgggtgc ttgttgcgag cgctgcgcgc
      601 gagcacggta gccaccaaaa acgcaacccc gctcgccatc catacgacca gagcgaacgc
      661 ggtccaattc atcgcttccg cgatgccctc cgccaagatg gttgctctct gggacgggtc
      721 gacgctctcg ccgcctactg cgccaaatgc tttcacgagc ccgaggcacg taccaatcgg
      781 acccaccaac ccgagcccag tcgccaaaac ggcgatgacc ttgttggcat tcgatagctg
      841 gctccttcgc cgccaaagtc gcaaagacag ccaaccggtg acgccgaacc ctacgagcac
      901 gagcgcgagc gaagcgagaa agacgccggc ggacgccatc cagtaagtct ttgccacgct
      961 ggctttccac tggcgaatcg gaggcatccg ggtaggggcg cggtgacgtc acgtctcagg
     1021 gcgccgtgtg gacccctctt ctcccgtgtt cgcggccacg ctcgggtggg tgcgacttct
     1081 accccgagga cccgagccgc aaaacatcga cggcgccgcg ttggtcgctt ggccgaacga
     1141 gtgcgtgagc tcggcggcga tttggtctaa gaaacgagtc gccgcggcgt tgtctagtcg
     1201 aggcgacacg aatgagcttg gtgggcactg atgacgttgc cggtcctgac tgggttctgg
     1261 ccgcgctgcg acagcacgag caaccgctgc tgcgctacgc cgcctcgttg gtaggtgaga
     1321 gtcgagcgca ggacgtcgtg caggatgcgt ttctgcggtt gtgctcgcaa tcgttcgaga
     1381 gcgtcgccga ccatcttgcg gcctggctat tcacggtgtg tcgcaaccgg gcgctcgagc
     1441 tacgccgcgc cgagcgtcga ctctcgtcga tggacgatgg cgacgaggac gacgcgcgcg
     1501 agtctcccga cagcgggccc atcgaaaagg tcgagcgcgc cgagaacgta tctcgcgtgc
     1561 gcgcggcgat ggctgctttg ccgaggcgcc accgcgaggt cttgcgactg aagctcgagg
     1621 cggacctcag ctacaaagac atcgccgcgg tcatgaacct gagcgtcggg aacgttggct
     1681 tcattctcca caccgcgatc gcgaaaatcc gagaacagct gagcgagccg ctcgaagcag
     1741 ccagggccga ggggagcgca ctatgagcat cgaaaagtcg gatccgaggc tcactgctta
     1801 cgcactggac gagctcagcg cggaggagcg gcgcgcgttc gaggcagagc tcgagcaaag
     1861 cgagggtgcg caacacgagc tcgtcgagat cgagcgcacc ctgcacgagc tgcggttcga
     1921 gttcttggat gccgatccgg tcaagctcga gagccgccgg cgcgtggaga ttgaggcggc
     1981 ggcgcagaca aaggttcgtg cgggccgcac gagccgccgg cgaaaaattt ggggcggtgt
     2041 atctctggga gcggtcgcgg cgagcgtgct tctgctgacg atgaagcatg
//
LOCUS       DASTKS010001197         5698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1309421, whole genome shotgun sequence.
ACCESSION   DASTKS010001197 DASTKS010000000
VERSION     DASTKS010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309421"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..456)
                     /locus_tag="VFQ35_25500"
     CDS             complement(76..456)
                     /locus_tag="VFQ35_25500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9934093.1"
                     /translation="MRPVVYDAGVLIAAERNERRVWAEHRVRLDAGLVPVVSACVVAQ
                     ASRSPKQAQMRRLLRGCEVVPFDENLAHRAGALLGKARTADVVDASVVALAVARGADI
                     LSGDLRDLRKLIATARAGTSVREV"
     gene            complement(453..611)
                     /locus_tag="VFQ35_25505"
     CDS             complement(453..611)
                     /locus_tag="VFQ35_25505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934094.1"
                     /translation="MTAAARHALLLREGLAAVAEWEAEHGAFTQAELDAAGRRIAQRG
                     RPERRRKR"
     gene            complement(739..1827)
                     /locus_tag="VFQ35_25510"
     CDS             complement(739..1827)
                     /locus_tag="VFQ35_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET9934095.1"
                     /translation="MSKLFSEFRLKDVSLKNRIVVSPMCQYSAEDGVPNEWHLVHLGS
                     RAVGGAALVIAEATAVSPEGRITPGCTGLWNETQAEAWAPIVKVVKRRGAVPGIQIAH
                     AGRKASANRPWEGDDHMAPSDPRSWEPLGPSAVAFGAYLPRVPREMTKAEIERVRSDF
                     VAAAKRALSVGFEWLELHFAHGYLAQSFFSPIANQRTDEYGGNFENRARFLIETLKAV
                     REVWPEKYPLTVRLGVSDFTKESQPLEESIELVKRFKSEGLDLIDVSLGFNTPDVSGV
                     PWGPGFMAPIAERIKGEARIATAVGWLINEPQQAEDIISKGQADLVMLAHKELDDPHF
                     PYHAAKALGVPNPENVLPSPYAHWLKGR"
     gene            complement(2012..2458)
                     /locus_tag="VFQ35_25515"
     CDS             complement(2012..2458)
                     /locus_tag="VFQ35_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934096.1"
                     /translation="MDLPSDLIDLLSAFAEEQVDYLLIGGQAVALHGVPRFTKDADLW
                     LRDTYDNLQRAKRALASFGAPAGTLRALDDARELDVVWMGHPPARIDLMKQVPGGDFD
                     AAYRARATFEVAGVTVSCVGKADLIAQKRASGRPQDLVDAANLERG"
     gene            2743..3294
                     /locus_tag="VFQ35_25520"
     CDS             2743..3294
                     /locus_tag="VFQ35_25520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET9934097.1"
                     /translation="MSSARNLPTLVLVALLPACAKEAPSQPPETEIRKVLAQQEDAWN
                     RHDAKAWVAAFSDDAEFINILGTSLQGRAEIERRHAEIFRTIFSHSHVSVQTRKVRAL
                     GSGAALAETDYQLRDYDRLPPGIRPTDPDGTLRTRLKYVWELRPEGWRIVSAQNTAIL
                     PSPPPALAAPPAQLAPPPVAPPG"
     gene            3372..3938
                     /locus_tag="VFQ35_25525"
     CDS             3372..3938
                     /locus_tag="VFQ35_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9934098.1"
                     /translation="MTGAGPELRFSFREYLSVDEATPVKHEYLDGMILAVAGGTPDHA
                     ALAMAVGASLTRQLLGQKCRVFSEALRIRVQATGFAGYPDVSVVCAKLMHDPEDANTV
                     VNPAVVVEILSPSTEHYDRGEKLRQYQLIPSLAHIVFVSHDSHRIEVVTRRDGAWSST
                     TAGPGGRASLSAIGCELDVDELYRDPLA"
     gene            3977..>5698
                     /locus_tag="VFQ35_25530"
     CDS             3977..>5698
                     /locus_tag="VFQ35_25530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934099.1"
                     /translation="MPAESLTESKPKSPSTLSNPPDNVRLALAALALAGPSNISAFTA
                     AAGAVGARESTARAFSTQTLRRAMDPYIEAGWVLDVGRGFECAYGLGPKVLRSYSPQA
                     LAGIGELYKTSVRKSYYYESPERARAALEVDVKLALAGAPLDFCSPLDRLLEAFGTID
                     VVEALLRDPLHGAFDPEWFARFSPPEQLKLARPFLDWCETRGVVLEGFGEYARAANSV
                     VQLDPDLRQTWARALLLLGGASALPAEVAGALPIAGGALFLRHVVAGDYAAARALLSR
                     AAGSKRQPLLSGAAGLFQVLVLFRNETLGDREDAIRLANLGARKGNLFRESFSVLKRL
                     LALQTGRKDSWFELLTQRPSSAADVLHELVQGLCVLWFKELELDLPYAWAAVQRAAVS
                     LERQPVTWLSRQYGDLEQALEAAAPSEAIATLRRAGHATRRGVEADDSATRTSLSAAD
                     ASRESSGASAISPLRQLFQQKPAWETALTRLEGLAQHADGSRDVAASLERVVWRVTAN
                     YVIEPYIQRATATGWSRGRRLAIKHLLPGASQRSTLPPEDLRVAEHARESVTQSYGYR
                     ETFHFFER"
BASE COUNT          975 a         2009 c         1715 g          999 t
ORIGIN      
        1 cgaagcggcg ttgggtcact gatcgcggaa aggtaaccgt cgcgaccgcc gcgaacggcg
       61 cggtccatcg cgctttcaga cctcgcgcac ggaggtgcct gcgcgcgcgg ttgctatcaa
      121 cttgcgcaaa tcgcgaagat caccgctcag aatgtcggca cctcgcgcga cggcaagagc
      181 gaccacactc gcgtctacca cgtccgcggt gcgcgccttg ccaagcaacg cgcccgcacg
      241 atgcgccaga ttctcgtcga aggggacgac ctcgcaacct cgcagcaacc gccgcatctg
      301 cgcttgcttt ggtgaacgac tcgcctgggc caccacgcac gccgaaacga ccgggacgag
      361 accggcgtcg agccggacgc ggtgttcagc ccacaccctg cgctcgtttc gctcagccgc
      421 gatcagcacc ccagcgtcgt acacgaccgg cctcatcgct tgcgtcgcct ttcgggtcgt
      481 cctcgctgag cgatgcgccg gcccgccgcg tcgagttcgg cctgcgtgaa cgcgccgtgc
      541 tccgcctccc attcggcgac cgccgcaagc ccctcgcgaa gcaacaacgc gtgccgcgcg
      601 gcggctgtca tccacccctc cccgatgcga ccaatgaacg aggccgcacg ccgctagcat
      661 gaagccgctc agcccgggac cgctcgcggt gcgcaggtga gcggaacaca ccggggggaa
      721 ctcaaagcgt tgcgttgctc acctgccctt caaccaatga gcatacgggg agggcaacac
      781 gttctctgga ttgggaacgc cgagcgcctt cgctgcgtgg tacggaaagt gaggatcatc
      841 gagctccttg tgcgcgagca tcacgagatc tgcctgccct ttgctgatga tgtcttcggc
      901 ttgctgcggc tcgttgatga gccagcccac cgccgtcgcg atacgagcct ctcctttgat
      961 gcgctcggct atgggcgcca tgaaccctgg tccccacggc acccccgaaa cgtcgggcgt
     1021 gttgaaaccg aggctcacgt cgatgagatc cagcccttcc gacttgaatc tcttcacgag
     1081 ctcgatcgac tcctcgagcg gttggctttc tttggtgaag tcgctgacgc caagacggac
     1141 ggtcagagga tacttctcgg gccaaacctc acgcaccgct ttcagcgttt caatcagaaa
     1201 gcgcgcacgg ttttcgaagt tgccgccgta ctcgtccgtc cgctggttgg cgatgggcga
     1261 gaagaaactc tgcgccaggt agccgtgcgc gaagtgcagc tccagccact cgaagccgac
     1321 cgacagggcc ctcttcgccg cagccacgaa atcactcctc actcgttcga tctcggcctt
     1381 cgtcatctcg cgtggcacac gcggaaggta cgcaccgaag gccaccgcgg agggcccgag
     1441 cggctcccac gaacgcggat cgctcggtgc catgtggtcg tcgccttccc acggccggtt
     1501 cgcggacgcc ttacgccccg catgcgcaat ctgaatccct ggtaccgcac cgcgacgctt
     1561 cacgaccttg acgatcggcg cccacgcctc tgcctgcgtc tcgttccaga gccccgtgca
     1621 gccaggagtg atgcgccctt ctggtgaaac cgcggtcgcc tccgcgatca cgagcgcggc
     1681 gccgcccacg gcgcgtgacc ccaaatgaac caagtgccac tcattcggca cgccatcctc
     1741 cgccgagtac tggcacatcg gcgaaaccac gatgcggttt ttgagcgaca catccttcag
     1801 gcgaaattcg gagaaaagct tcgacatggc acccgaatat gcccgatcgc ggcacccgcg
     1861 acgagttccc gccatcctcc ccttcgccgc tttcgtcgcc tcgccgcttt cgtcgcctcg
     1921 ccgccttcct cgcctccgtc gccttcgtcg ccttcgcctc cccccaacct ccgccgccga
     1981 tggctgcagc ggctcggatg ccagaaccgc gctacccgcg ctcgaggttg gccgcatcca
     2041 cgagatcttg ggggcgaccc gaagcgcgct tttgcgcgat gagatctgcc ttgccaacgc
     2101 aagagaccgt cacgcccgcg acctcgaacg tcgcgcgtgc gcgatatgcg gcatcaaagt
     2161 ccccccctgg cacctgtttc atcaagtcga tgcgtgccgg cggatgcccc atccacacca
     2221 cgtccagctc gcgggcatcg tcgagcgcgc ggagcgtccc ggcgggagcc ccgaacgacg
     2281 cgagagcacg cttcgcgcgc tggagattgt cgtacgtatc acgcagccaa aggtcagcat
     2341 ccttcgtgaa acgcgggacc ccgtgcaaag cgacggcttg ccccccgatc aagaggtagt
     2401 caacctgctc ttctgcgaac gccgaaagca gatcgatcaa gtcggagggg aggtccattg
     2461 ggatgctcgt aatgccacca ttgattggcc atttgccaaa ccgctgccaa acgctcggcc
     2521 gccgtggtct ggctccaaaa ctggcgatcg agatccgccg actgcgcggc ggaaacgagt
     2581 ccttgagccc attgcgtgcg acgctgggcg gctctttgtt cacgtgacac gcttgccatc
     2641 ataacacgca gagcggctcg ccgcgcagcc ggcaactgcc tcaactgggc cgaagggcat
     2701 ccacgtaccc ctcacgcccc ctttctgctt tgatgcgccc ccatgtcgtc ggctcggaac
     2761 cttcccactc tcgttctcgt cgctttgctg cctgcttgcg ccaaagaagc gccttcccag
     2821 ccacccgaga ccgaaatccg caaagtcttg gcacagcaag aagatgcgtg gaaccgacac
     2881 gacgcgaagg cctgggtagc ggcgttcagc gacgacgcgg agttcatcaa catactgggc
     2941 acctcgctgc aggggcgcgc cgaaatcgag cgacgtcacg cagaaatctt ccgcaccatc
     3001 ttctcgcact cccacgtcag cgtccaaact cgcaaagtgc gcgctctcgg ctcgggcgcg
     3061 gcgctcgccg aaacggacta ccaacttcgc gactacgatc gcttgccgcc cggcattcga
     3121 cccaccgacc ccgacggcac gttgcgcacc cgactcaagt acgtctggga actaagaccc
     3181 gaaggctggc gcatcgtctc cgcgcaaaac accgccattt tgccgtctcc gccgcccgcg
     3241 ctggcagctc cacccgcgca gcttgcgccg ccgccggttg cgccgccggg ttagctctag
     3301 gcttccgggg agctcgccgc cgcggctgtt ggcggccttg ggcgaaccac gcgacatgct
     3361 aacgttcctc ggtgaccggc gccgggcccg agctccgctt ctcattccgc gaatacctca
     3421 gcgtggatga ggcaactccg gtcaagcacg agtatctcga cggcatgatc ctcgcggtgg
     3481 caggcggtac accggaccat gccgcgctcg ccatggcagt cggtgcctcc ttgacccgcc
     3541 agttgctggg ccaaaagtgc cgcgtcttca gcgaagccct acgcatccgc gtgcaagcca
     3601 cgggattcgc tggctatccc gatgtgagcg tcgtgtgcgc gaaactcatg cacgaccccg
     3661 aggacgcgaa caccgtcgtc aacccggccg tcgtcgtcga gatcttgagt cccagcacag
     3721 agcattacga tcgcggggag aagctccgcc agtatcaact catcccgtca ctcgcgcaca
     3781 tcgtgttcgt ctcccacgac agccatcgca tcgaggtcgt cactcgacgc gatggcgcct
     3841 ggagttcgac gactgccggc cccggtggcc gcgcctcgtt gtcggccatc ggttgcgaac
     3901 tcgacgtcga cgagctctac cgggatccgc ttgcgtaatg gttgcgtcag cgcgcgagcc
     3961 gccttagggc aacgacgtgc ccgccgagtc actcaccgaa tccaaaccca agtcgccgag
     4021 cacgctgtcg aatccgcccg acaacgttcg gctcgcgctc gcggcgctcg cgctcgccgg
     4081 cccatcgaat atcagcgcgt ttacggccgc agccggcgcg gtcggagctc gtgagtcgac
     4141 ggcgcgagcg ttttcgacgc agacgctgcg ccgcgcgatg gatccttaca tcgaagccgg
     4201 atgggtcctg gacgtcggtc gcggtttcga gtgcgcgtac ggattgggcc ccaaggtgct
     4261 gcgctcctat tccccgcaag cgctggccgg gatcggcgag ctctacaaga cgagtgtacg
     4321 taagtcctac tattacgagt cccctgagcg cgctcgcgcc gccctcgagg tcgacgtcaa
     4381 gctcgcgctc gccggcgcgc ctttggactt ttgctcgccg ctcgaccgac tgctcgaagc
     4441 gtttggtacc atcgatgtag ttgaggcact ccttcgcgac ccgctccacg gtgcgttcga
     4501 tccagagtgg ttcgcgcgtt tctcgccgcc ggagcagctg aagctcgcgc gtccgttcct
     4561 cgactggtgt gagacccgcg gcgtcgtgct ggagggcttt ggcgagtacg cccgcgccgc
     4621 aaactcggtc gtgcagctcg atccggacct gcggcaaacc tgggcgcgcg ctctcttgtt
     4681 actcggagga gcgtccgcgc tgccagcgga agtggcggga gcgctgccga tcgcgggcgg
     4741 ggcccttttt ctgaggcacg tcgtcgccgg cgactacgcg gcggcccgcg cgcttctttc
     4801 gcgagcggcc ggcagcaaac ggcaaccgct gctctcgggc gcagctggct tgttccaagt
     4861 tttggtcctg ttccgcaacg aaacgctggg tgaccgcgaa gacgctattc gtcttgcaaa
     4921 cctgggagca cgcaagggga atctctttcg agagtctttc tcggtgctca agcgcctgct
     4981 cgcgctgcag actggaagga aggactcgtg gttcgagcta ctcacgcaac gcccctcctc
     5041 cgccgcggac gtgctgcacg agctcgtcca ggggctttgc gtgctgtggt tcaaagagct
     5101 cgagctggat ctcccttacg cctgggcagc ggtgcaacgc gcggcggtgt ccctcgagcg
     5161 gcaaccggtc acctggcttt cgcggcaata cggcgacttg gagcaagccc tcgaagccgc
     5221 cgcgccgtct gaagccatcg ccacgttgcg acgggcgggt catgccacgc gaaggggcgt
     5281 ggaggcggac gactccgcga ctcggaccag tctctctgca gccgatgcga gccgcgagtc
     5341 ttccggcgcg tccgcgatct cgccgttgcg gcagctgttt cagcagaagc ccgcgtggga
     5401 aacagcgctc acgcgactcg aaggcttggc gcaacacgca gacggctcgc gcgacgtggc
     5461 cgctagcctc gagcgcgtgg tctggagggt gacagccaac tacgtcatcg agccctatat
     5521 ccaacgcgcg acggccaccg gatggagtcg cggaaggcgt ctcgccatca agcacttgct
     5581 gcccggagcc agccaacgca gcacgttgcc tcccgaagat ctgcgggttg ccgaacacgc
     5641 gcgcgaatcg gtgacgcaga gctatggcta ccgcgagaca tttcatttct tcgagcgc
//
LOCUS       DASTKS010001198         5704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1980121, whole genome shotgun sequence.
ACCESSION   DASTKS010001198 DASTKS010000000
VERSION     DASTKS010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980121"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFQ35_25535"
     CDS             <1..331
                     /locus_tag="VFQ35_25535"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9934100.1"
                     /translation="KHTYRFLSKIPWGKTFARVAAIAGAHHERLNGTGYPNRLRAEEI
                     PLQSKLMSISDIFDALTATDRPYKRAVPIQKALDILGFEVKDGHLDGDLVRIFREARV
                     WEPVMGY"
     gene            complement(1438..2046)
                     /locus_tag="VFQ35_25540"
     CDS             complement(1438..2046)
                     /locus_tag="VFQ35_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934101.1"
                     /translation="MDTAPAVCAHEAPAASDYLDRLMRRNSFEALWPLFAKAQRPAKE
                     LTESYSALVHLRPFLQKREGCRVVHVGDGAHARTAAMFSLKSKTYNISVDPELNLALV
                     DDWRQRFGVERFERRRARIEDVAQELNTSSDVPTFVTFVHAHVEVEQVLARLRWDAAF
                     VLACCVPGKQLCRSHVRFEGEDMNVLSLGRRYQVLVNESRSR"
     gene            2281..3318
                     /locus_tag="VFQ35_25545"
     CDS             2281..3318
                     /locus_tag="VFQ35_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934102.1"
                     /translation="MRARFSARTTIASLWLFSAARSWADEPPAALVVVDWRAPASCPA
                     REAILDEANRLLGDAPRASASSLVARGRIVRETKSYVLELELDQDRRTTRRRLVAHAC
                     PELGRAAALVLALAVDPSLVSRNEAAEPGALLECPPTPEPVCPNPEPVASSERSCAPK
                     PEPTCPALHAPAPRPEPPGDAPRNFHVGGGVRIATGELPETLPSPELYAGYGTAPFRV
                     DLAARFASAHSTVSAGAASFQSWTLRPRLCFGRPLRELVASVCADVSLGRVRGEGAGA
                     DVVHTRWSNWVAPGLEVLLELPVGSSAVGLSLAGELPLVRTKFELAGARVFEPAKLVA
                     SAGLVGTIGLF"
     gene            3371..3949
                     /locus_tag="VFQ35_25550"
     CDS             3371..3949
                     /locus_tag="VFQ35_25550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9934103.1"
                     /translation="MAPELARDLRAGPVTPPPAQPLDVAHIYEAHFDFVWRSLRRLGV
                     PSSALEDALQDVFIVVQRRLVDFEGRSSLSTWLFGIVLRVATEYRRRNARRNHEISTD
                     DAVLIGTSRDPYDAARETEAARLVQEVLDAMADERKVVFVMIELEDLAPLEVAEQLGI
                     GVNTVYSRLRLARRDFEAHVKRVRARELWRLP"
     gene            3946..4656
                     /locus_tag="VFQ35_25555"
     CDS             3946..4656
                     /locus_tag="VFQ35_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934104.1"
                     /translation="MSDDDREVMALLAAARRVGPPAHTKDRLRARVLAGVTSGAALSS
                     ATVAAKGAAVAAASGSAIASKAVIALVVGVALGLGGSVIASSVASSPAAVKRMATQTI
                     VSAPKPVTSARPVEKTEVAAPKEATAVPSALPSAEPKRAAAPLHTPSIDDETRLIASA
                     HRALASGRANDALAALNRYRQEFPNGALLEEATGARVLALCAAGRKAEGEAARASFLR
                     AFPNSPLSTRVGSACVTP"
     gene            4710..>5704
                     /locus_tag="VFQ35_25560"
     CDS             4710..>5704
                     /locus_tag="VFQ35_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934105.1"
                     /translation="MERTIIGLGFWGVSYVLGAVSLLAGCGEVKSLGENMGGNAGTSA
                     LGSGGVAGVGADTSPVGSGGAAGAGAGGTHAGTGGSATGTPYPLAPSVPVGRNCGCDE
                     ESQVCNAASECVPRCDAEGHCAKWLLDREVSDFYVDVGTLLFLEAPTRDRLGNLLDKP
                     QHLMYADFTLDSPIWRVELPPSSESSILGHWNHVTYVRAGGIYAVPDDVAPGSTVENM
                     RIETPDTIYAAAFAQGTLYFAAPGGIWQMNVDTEPTPRHILDVDYGASTNYANLVIGD
                     ALWFQNGKADQVCVFSSAGPSVECKPTQGGGFAFPQAAHGNDAFVGEYHGSVFRER"
BASE COUNT          974 a         1861 c         1842 g         1027 t
ORIGIN      
        1 gaagcacacg taccgctttc tctccaagat cccgtggggc aaaaccttcg cgcgtgtcgc
       61 cgccatcgcc ggcgcacacc acgagcgcct gaacggcacc ggctatccca accgactgcg
      121 cgccgaagag atcccgttgc aatcgaagct catgagcatc agcgacatct tcgacgcgct
      181 gaccgcgacc gaccgccctt acaagcgcgc agtcccgatc caaaaggcgc tcgacatact
      241 cggcttcgaa gtcaaagacg gtcacctcga cggcgacctc gtgcgcattt tcagagaagc
      301 gcgcgtgtgg gagcccgtga tgggctactg acgagcgcgt tcggcgacgc ggcgatgggc
      361 cattggcaga actcgcggca tcgtgatgca gaacagcttc cacgatgccg cgaggttcct
      421 tcaatcgatc agcaaggcgc gcggctggac cacccgtttt cggtgcacag gtaaagggtg
      481 tggtcgagtc cgcaacaggt ttgtccgacg gctctctcac agttcatcgt cggacttctt
      541 ccgcaatagc cggtcgaggt accgagcgag cacccgggat gctgcgcaca gtctccggaa
      601 gagaagatat tgcagttgac cgtcgtggga gggacggagg tgtacgcgcc gccggagcca
      661 gagcagatct gatacgagta ctgatagcag ttgcctcctc cacacgcggt gcccgtgtaa
      721 gtttgcaagc tgcacccgtt cgcgctcgtg cattgtgctg gttgcaatcc cggacaaggc
      781 acgtattggt agctcgcggg aagggtcgtc tgcccgacgc aagcggacga aatctcggcg
      841 gtggatttcg ccacgtccgt tgattcgttg gcttccgtcg agccagggtc tgcgccgcaa
      901 gcggcgagag cgagcgcggg aattccgcag gcgagtgaac gtcgaaccag gcgcccgacg
      961 aaaccttttt tcatcactgc gggggaacgg gaatccttgt gcttggtcat caatcacatc
     1021 tccttcgaac gtatcgttcg tgactcagcg cgcgcctaaa tctcggactc tctggctgcg
     1081 gcgtcgcacg gcacatcatg ttcaggaaga cgtgcttcga tctcgctacc gctcattttt
     1141 gcaaaccaga acggaggcgt ctccttaggg cccgcgaatg gctaagtcga tcgatctcgc
     1201 ggccgatcca tcccggctgg cttcgttgct cctcagttac atactgcaag tatgcgcctt
     1261 cgtcgcgcct cgccagccgg gcgcctcgac cacgatcttc gaccgactta gccattcgcg
     1321 ggcccttagc gaatttcgcc gcgcgccttc acggcgtgca tcaggcgttt gttctgcttt
     1381 ccgaacctcc ccagggcgag aagcgggcct aaaagtgcgg cgtgtaattc gtcgtgctca
     1441 gcgcgaacgg ctctcgttca ctagcacctg gtatctgcgt ccgagcgaga gcacgttcat
     1501 gtcttcgcct tcgaagcgta cgtgagaacg acacagctgc tttcctggta cgcagcacgc
     1561 aaggacgaac gccgcgtccc aacgcaaccg cgccagcacc tgctccacct cgacgtgtgc
     1621 gtggacgaac gtaacgaacg tcggaacgtc gctcgacgtg ttcagctcct gcgcgacgtc
     1681 ttcgattcgc gctctgcgcc gctcgaaacg ctcgacgccg aagcgctggc gccaatcgtc
     1741 gacgagcgca aggttgagct ccgggtcgac gctgatgttg taagtcttgc ttttcagcga
     1801 gaacatcgcc gcggtcctcg cgtgcgcgcc gtcgcccacg tgcacgacac ggcagccctc
     1861 tctcttttgc aggaacgggc gcaagtgcac gagcgcggag tagctctcgg tcaattcttt
     1921 cgcggggcgc tgggccttgg cgaacagcgg ccacaaagcc tcgaagctat tgcgtcgcat
     1981 caaccggtcc aagtagtcgc tcgccgcagg cgcctcgtga gcacacacgg ccggggctgt
     2041 gtccatgacc gctcctacca gcttggtgca cctcgcacca aagctcatcg tgggccggcg
     2101 cgacgccgtc gccctccgtc cgtcatcgtc gcgggtcgtc gcgctgggac agtgaaagcg
     2161 ctcgacatgc tcgcgcccat cttcagcgaa gcgcgcgtgg gggagcctgt gatgggtcac
     2221 tgatgatctg cggcgcgaat ttcagttacg aagccgccac gtgtgtgaca ttctcggcgc
     2281 gtgcgagcgc ggttctcggc gcggacgacc attgcaagcc tttggctgtt ctccgcggct
     2341 cgaagctggg ccgacgagcc gcccgcggcg ctcgtggtcg tggattggcg tgcgccggca
     2401 agctgccctg cacgcgaggc gatcctcgat gaagccaatc gactcctcgg tgacgccccg
     2461 cgggcgtcgg cgtcctcgct cgtagcgcgt ggacgcatcg ttcgtgaaac caagagctac
     2521 gtgctggagc tcgagctcga tcaggatcga cgaacgacgc ggcggcgcct ggttgcacac
     2581 gcctgccccg agctcggtcg ggcggcggcg ctggtcctcg cgctcgccgt cgatccgtcg
     2641 ctggtctcgc gaaacgaagc ggcagaaccg ggcgcgctgc tcgaatgtcc accgacacct
     2701 gagcccgttt gcccaaaccc tgagcccgtc gcgagcagcg agcggtcgtg cgcgccgaag
     2761 ccagagccaa cgtgccccgc gctccatgct cccgcgcctc gaccggagcc acccggcgat
     2821 gcgccacgca atttccacgt gggcggggga gtgcggatag ccaccggcga gttgccggag
     2881 acactgccga gccccgagct ctacgcaggt tatgggacag cgccatttcg cgtcgacctc
     2941 gcggctcgct tcgcctcggc ccattccacc gtgagcgccg gggccgcctc cttccagtcg
     3001 tggacactgc ggccgcgctt atgctttgga agaccattgc gtgagctcgt tgcaagcgtg
     3061 tgtgctgacg tgagcctcgg gcgcgtacgc ggggagggcg ccggcgccga cgtagtccac
     3121 actcgctggt cgaattgggt cgctccgggg ctcgaggtgt tgctcgaact gcctgtcggc
     3181 tcctcggccg tggggctctc tctcgcaggt gaattgcccc tcgtgcgcac gaagttcgag
     3241 ctcgccggag cgcgggtctt cgaaccggcc aagctcgttg cgagcgcggg tttggtgggc
     3301 acgatcggcc tattttgagc gactctccgt tttcgtgacg aacacgcgta gctcccgcca
     3361 ctcaaccccg gtggcccccg agctcgcccg cgatctgcga gcaggtcccg tgactccgcc
     3421 ccctgctcaa cctctcgacg tcgcacacat ctacgaggcg cacttcgact tcgtgtggcg
     3481 gagccttcgc cgactcggtg ttccgtcgag cgcgctcgaa gacgccttgc aagacgtctt
     3541 catcgtcgtg cagcgccggc tggtcgactt cgaaggacgc agcagcttga gcacgtggct
     3601 cttcggcatc gtgctgcgag tcgcgaccga ataccggcgg cgcaatgcgc gacgcaacca
     3661 cgagatttcg acggatgacg cggtgctcat tgggaccagt cgcgatccct acgatgcggc
     3721 gcgggaaacc gaagccgcgc ggctcgttca agaggtgctc gatgccatgg ccgacgaacg
     3781 caaagtggtc ttcgtcatga tcgaactcga agacttggcg ccgctcgaag tggccgaaca
     3841 gctcggcatc ggcgtaaata ccgtgtattc gcggctacgc ctcgcccgac gcgacttcga
     3901 agcgcacgtg aagcgggtgc gcgcccgcga gctttggagg ctcccatgag cgacgacgat
     3961 cgcgaggtga tggcgttgtt ggcggcggct cgtcgagtcg gcccgcctgc gcacaccaaa
     4021 gatcgactgc gggctcgagt tctcgccgga gtcacgtcgg gcgccgcgct ctcgagcgca
     4081 accgtcgcgg ccaagggcgc agccgtcgcg gccgcaagcg gatccgccat cgcgagcaaa
     4141 gccgtgatcg cgctcgtagt gggtgttgcg ctcggactcg ggggttccgt catcgcatcg
     4201 agcgtcgctt cgtcgccggc ggcggtgaaa cgcatggcga cgcaaacgat cgtgagcgca
     4261 cccaagcccg tgaccagcgc gcgccccgtc gaaaagaccg aagtcgccgc acccaaagaa
     4321 gccacggcgg ttccgtccgc tttgcccagc gccgagccga agcgagcggc ggcgcccctg
     4381 cacacgccgt cgatagacga cgagactcgg ctcatcgctt cggctcatcg cgccttggcg
     4441 agtggccgtg cgaacgatgc gctcgccgcg ttgaatcgct atcgccagga atttccaaac
     4501 ggagcgctgc tcgaagaagc gacgggagcg cgcgttctcg cgttgtgcgc cgcaggtagg
     4561 aaagcggaag gggaagccgc gcgcgccagc ttcttgcgag cgtttccgaa ttcgccgctc
     4621 tcgacgcgcg tcggctcggc gtgcgtgacc ccgtgaacga tttcgtgatg aagacggcct
     4681 cgcaacgcac tcacgatcca gaggacgcaa tggaacgaac aatcatcggg cttggttttt
     4741 ggggggtgag ctacgtgctt ggcgccgttt cgctgctcgc tggatgcgga gaagtgaagt
     4801 cgctcggcga gaacatgggc ggcaacgccg gcacctcggc gctggggagc ggcggagtcg
     4861 caggtgttgg cgccgacacg tcaccggtgg ggagcggtgg tgctgcaggt gctggcgctg
     4921 gcggcactca cgcggggacg ggcggcagtg cgaccggcac tccgtatccg ctcgcgccga
     4981 gcgtgccggt cggccgcaac tgcggctgcg acgaagagtc gcaggtttgc aacgccgctt
     5041 ccgaatgtgt tccgcgctgc gacgcggagg ggcactgtgc caagtggttg ctcgaccgcg
     5101 aggtcagcga cttctacgtc gacgtcggga cgctcctctt tctcgaagcg ccgacccgcg
     5161 accggttggg caacttgctc gacaagccgc aacacctcat gtacgcggat ttcaccctcg
     5221 attccccaat ttggcgcgtc gagctcccgc cctcgagcga gagctccatc ctcggacact
     5281 ggaatcacgt cacttacgtg agggcgggag ggatctacgc cgtgcctgat gacgtcgcgc
     5341 ctggctcgac cgtggaaaac atgcgcatcg agacgcccga caccatttat gcggcggcgt
     5401 tcgcgcaggg cacgctgtac ttcgcggcgc cgggcggcat ctggcagatg aacgtcgata
     5461 cggagcccac gccgcgacac atcctcgacg tcgactatgg cgcgagcacg aactacgcaa
     5521 acctcgtgat cggcgacgcg ctgtggttcc aaaacgggaa ggcggaccaa gtctgcgtgt
     5581 tctcgagcgc aggacccagc gtcgagtgca agccgacgca aggcggtggc tttgcgtttc
     5641 cccaggccgc gcacggcaat gatgctttcg tgggtgagta tcacggctcg gttttccgtg
     5701 agcg
//
LOCUS       DASTKS010001199         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1755509, whole genome shotgun sequence.
ACCESSION   DASTKS010001199 DASTKS010000000
VERSION     DASTKS010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755509"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFQ35_25565"
     CDS             <1..218
                     /locus_tag="VFQ35_25565"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9934106.1"
                     /translation="GARMLHAELEQAMIEEGDYDRPILVYCYRGNESKKKADYLAQRG
                     FKRVYSLDNGFTGWPRSKPEEQVSLQG"
     gene            223..1806
                     /locus_tag="VFQ35_25570"
     CDS             223..1806
                     /locus_tag="VFQ35_25570"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9934107.1"
                     /translation="MSAQSSTARVEGTDRVERLRARIEELGRDFAANVALATAAHVRP
                     VDEAELAGMAPDDRERARRRATARGLEGHALTLDAPSYRAALASLDDRGLRKTLYEAY
                     MTRASDRGPYAGQFDNTPLLSEILELRYELARELGFKNYAELALSEGVLRDPDEAEAR
                     LVSHHRQTKSRAQAELDELWAFAKEKGVPRGFSPWDLPYYAAWWSRQNFASTEAEVRR
                     YFSLQGVLAGAMELAAALTGVSFVEMETSAQHAKRRYRVANEAGVTVGFLMFETHATD
                     SLLTNARVSLLPDTEDGLRVVQVQCDLETPNLADPGPLSLGQIEALLRGLGSALFLLL
                     AQPATSARPQDQVGAQVAGAYLQLFASRFDTLQDFARRSATDSEFPLALFDDLTKRRE
                     THAGLAASEELELSLFDLRIHRDHIPRHKATQLRVQVLDTFTQIRREQSVLPHSYWTR
                     IANFDLAIFVDGRAARLWERDFARAMATELFALTGKSEGAVGTMRHLRSTFWATAERP
                     LLSRLADALGYAQTFGANR"
BASE COUNT          371 a          677 c          627 g          329 t
ORIGIN      
        1 aaggagcgcg catgctgcac gccgaactcg aacaagccat gatcgaggaa ggcgactacg
       61 accgacccat cttggtctat tgctaccgcg gcaacgagag caaaaagaag gccgattacc
      121 tggcgcagcg cgggttcaag cgcgtttata gcctcgacaa cggcttcacg ggttggccgc
      181 gctcgaagcc ggaagagcaa gtctctctcc aggggtgaag ccgtgagcgc ccaatcgagc
      241 acggcgcgcg tagaaggcac cgaccgcgtc gagcgccttc gtgctcgcat cgaggagctc
      301 ggccgcgact ttgcggcgaa cgtggcgctg gcgacggcgg ctcacgttcg accggtcgac
      361 gaagccgagc tcgctgggat ggcgccggac gatcgcgaac gcgcgcgccg tagagcgacc
      421 gctcgcgggc tcgaaggtca cgcgctgacg ttggacgcgc cgagctatcg cgcagcgctc
      481 gccagcttgg acgaccgagg gctgcgcaag acgctgtacg aagcgtacat gacgcgcgcg
      541 tccgaccgcg gcccctacgc gggacagttc gacaacacac cgctcttgag cgagatcctc
      601 gagcttcgct acgagcttgc gagagagctc gggttcaaga actacgctga gctcgccttg
      661 agcgagggtg tgctgcgcga tcccgacgag gccgaagcgc gtttggtgtc gcaccatcgc
      721 cagacgaagt cacgagccca ggccgagctc gacgagctct gggctttcgc caaggaaaaa
      781 ggcgttccac gcggcttctc tccgtgggac ctcccctact acgccgcctg gtggtctcgc
      841 caaaacttcg cctcgacgga agcggaggtt cgtcggtact tctcgttgca gggagtgctc
      901 gccggagcga tggagctcgc cgccgcgctc acgggcgtgt ccttcgtcga aatggagacc
      961 agcgcgcagc acgcgaagcg ccgctaccgg gttgccaacg aagccggggt taccgtcggc
     1021 tttctgatgt tcgagacgca cgccacggac agccttctca ccaacgcgcg cgtctcgttg
     1081 ctgcccgata cggaggacgg tttgcgcgtc gtgcaggtgc agtgcgacct cgaaacgccg
     1141 aacctggccg atcccggccc gttatcgctc ggccaaatcg aggcgctgct gcgaggcctc
     1201 ggaagcgcac tcttcctcct gcttgctcag cccgcgacct ccgctcgtcc gcaagaccag
     1261 gttggcgccc aagtggcggg agcgtacctg cagcttttcg ccagccggtt cgatacgttg
     1321 caggacttcg ctcgccgcag cgcaacggac agcgaatttc ccctcgctct gttcgatgac
     1381 ctcacgaagc ggagagagac ccacgcgggc ctcgccgcgt cagaagagct cgagctctcg
     1441 ctcttcgatc tgcgaattca ccgcgatcac atcccacgcc acaaggcgac gcagctgcgc
     1501 gtacaggtcc tcgatacgtt cacgcagatt cgacgtgaac agagcgtctt gccgcactcg
     1561 tactggaccc ggatcgccaa cttcgacctc gccatcttcg tagacgggcg ggccgcacga
     1621 ctgtgggaac gcgattttgc gcgtgccatg gccacggagc tcttcgctct caccggaaag
     1681 agcgaaggcg ccgtcgggac gatgcggcac ttgcgctcga cgttctgggc gaccgccgag
     1741 cgaccgctgc tctcgcgtct cgccgacgct ctcggctacg ctcaaacctt cggagcgaat
     1801 cgctaaagcc cgatcgaaat ggccgagatt acctgatgcc cgaggcgaag cacgacgcag
     1861 accagccaga gcccagcgga atcgtgcaca gcgcgcccac cgcgcttccc tgcgaaatcg
     1921 atttcactcc ggatgacgta gctcccccga gctacgggcg cgccacgttg ccgccctggc
     1981 tcatcgcttc gcacgagttc caga
//
LOCUS       DASTKS010001200         6328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1310175, whole genome shotgun sequence.
ACCESSION   DASTKS010001200 DASTKS010000000
VERSION     DASTKS010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310175"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFQ35_25575"
     CDS             <1..890
                     /locus_tag="VFQ35_25575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9934108.1"
                     /translation="KGAQKLKLALNWVPEPEFGGFYAARESGAYKRRGIDLEILGGGA
                     GVPVIQMVATGRADFGTVGADEVIGGRVRGADVVAVFATYQTFPQGIMVHAGRKLTKI
                     EQAFQSGTIALQTGVAYSTYLKQKFSWEGAQVVPYDGGVARFLSDPQFSQQCYVTSEP
                     LAAKKQGGDPQVFLIADTGFNPYATVVITRSELLKKSPELVRSFVQASAEGWKAYLQD
                     PGPTNQKLGELNKSMDAASLRESAEAQKPFIETDDTRQHGLGSMRRDRWETLTNQLFD
                     LKLVPQKPNVDELFVNFPP"
     gene            complement(903..1418)
                     /locus_tag="VFQ35_25580"
     CDS             complement(903..1418)
                     /locus_tag="VFQ35_25580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934109.1"
                     /translation="MTARKTTNSIRPGLHVLIVDDDPNVLRDVSTRLNRENHRVSVFS
                     RASGVRQLVRVLKPQVVLIDVLMPDMNKRILLELLSACRRSEEQPIVILHSRLSVRML
                     KTLVNVGNAAGVIQKTKSDIEFMLAFNAIVERARAPHARFLSPSSTSGTHRIPTLADE
                     DVEELRTGSTR"
     gene            1785..2291
                     /locus_tag="VFQ35_25585"
     CDS             1785..2291
                     /locus_tag="VFQ35_25585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934110.1"
                     /translation="MPARTHAPTLVLSLCLAACSALGGGDTGLRDELAASRAEAQAEH
                     ERVQELEQRLAQLELNSKVQVRDRRIEARLERLIDQNEALLRAQSAPACQPSAPQSTT
                     EPSRPPTGTASFADATEGDQKKQLEERLRAFVRGQGGGLSAEQREAMRVLLRKDRVLD
                     RANPWHDN"
     gene            complement(2341..3714)
                     /locus_tag="VFQ35_25590"
     CDS             complement(2341..3714)
                     /locus_tag="VFQ35_25590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934111.1"
                     /translation="MNERFAPGQVLAGKLRIVRLLGAGGMGAVFEVEHELTRHRRALK
                     LLHPQMAGIPSVVERFLREASAAGRIGNRHIVETFDAGRLETGEPYIVMELLTGRTLG
                     EVLEQRGPLSLDAACDIVIQACDAVSAAHAAGIVHRDLKPENLFLCGSDASFVKILDF
                     GVSKFDSATTGVDGITLEGSPIGTPFYMSPEQVRGEKNIDARADVYALGVLFYECLTG
                     RKPFVAETLPHLAVLIYQGKYATPSEVRNGLPPAVDLVVSRAMASDRTERFASADELS
                     SAVSRLRSSLAPGFMGQTQPLVQSEPPIAPPRPMPALTPAVFTSPPSDVVKPRSRLWI
                     PLAVVALAGLGVVGAQYARTRSEAASSAEPPSVNVPTHTAVVPVVNEDAAPRVEPSAS
                     PLASASPSAAAPVHGKGAVSVVPIAPSAAAAASAVAAPSAQPVGSVTTPRSRASSYGL
                     SQENPFK"
     gene            complement(3988..5985)
                     /locus_tag="VFQ35_25595"
     CDS             complement(3988..5985)
                     /locus_tag="VFQ35_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934112.1"
                     /translation="MVLSLGLRPGRIILLSSVLSLTAWACSDSDKEKIQAKGLSAGCT
                     LNSECSDPLVCTFARCHQECAQDRDCKTGEERCVKGATGFVCQLAVETDCTKDKTVCK
                     GTQVCGIDGECRDACTTSTDCTPGQVCAPSGQCASTDPKKDTLDSSGNILPDPFVDSS
                     GGTGGTGGTGNTNKGGATGKGGATSSGGGPEMSGGAGGDLPIEMSGGKTSTGSGGKTA
                     GFGGAPGAGGSVVTTGGAGGEGGTVPFECPPGKGECDPTDPTDCETFLTLATSCGACG
                     VSCGSVHGTSVCDAATLTCKVMGACTQGYADCNTSGVDGCEVTLATDPKNCGTCGRDC
                     GGGTCTNGQCGAAIVFDPTGATTLTYNYTGSAVLTGSTLVKVNTSSGTEIRVATLPTT
                     PDTLKGTALVTNQSNVYIYGLHADAANVYYSLSGSPNATVLWKPLTAMETSTPKTAVT
                     MPDTYYAKIITSNATALYMVNAANVILAAGKSLTAAQPASPPSGLTTARSTVNDLVVA
                     GGYLYWLEAPNVVYAYSLVNGGAPVAVDSTTQSGYSGYQGIATDGSYVYWSTYNGSSS
                     KIRRLAVSAAPSPDAVQDVAIGIAAPSSGLAVDANYVYYYYQNATVWRVVKDGSITPT
                     QIGTVNATPYFYSMFAVDSGYVYGVGGSGQIVRVAKAPSGG"
BASE COUNT         1127 a         2163 c         1954 g         1084 t
ORIGIN      
        1 ccaaaggcgc gcaaaagctc aagctcgcgt tgaattgggt gcccgagccc gagttcggtg
       61 gcttctacgc ggcgcgcgag tccggcgctt acaagcgccg cggcatcgat ctcgagatcc
      121 tgggcggcgg cgccggtgta ccggtgatcc aaatggtcgc gaccggccgc gccgatttcg
      181 ggaccgtcgg tgccgatgaa gtcattggcg gccgtgtgcg aggcgctgac gtcgtcgctg
      241 tcttcgccac ctaccaaacc ttcccccaag gcatcatggt gcacgccggg cgcaagctca
      301 ccaagatcga gcaagccttc cagagcggca ccatcgcgct acagacgggc gtcgcctact
      361 ctacttacct gaagcaaaag ttctcttggg agggcgcgca agtcgtgcct tacgacggcg
      421 gcgtggcgcg tttcctgagc gatcctcaat tttcgcagca gtgctacgtg acgagcgaac
      481 ccctcgccgc gaagaagcaa ggcggcgacc cgcaagtctt tctcattgcg gatacggggt
      541 tcaatcccta cgccacggtc gtcatcacgc gcagcgagct cctgaagaag agccccgagc
      601 tcgtgcgctc cttcgtgcaa gcgagcgccg aaggctggaa ggcctacttg caggatcctg
      661 gtcccacgaa ccaaaagctc ggcgagctca acaagtcgat ggacgccgcg tcgttgcgcg
      721 aatccgcaga ggcgcaaaag cccttcatcg agaccgacga cacgcggcaa catgggctcg
      781 gaagcatgcg gcgcgatcgc tgggaaacgc tcacgaacca gctcttcgac ctcaaactcg
      841 tcccccaaaa gcccaacgtc gacgagctct tcgtcaactt cccgccgtag aacgcaccgc
      901 gctcagcgcg tgctgcccgt gcgcagctct tcgacgtctt cgtcggcgag ggttgggatg
      961 cgatgcgtcc cactggtgct gctcgggctc agaaatcggg cgtgcggcgc gcgtgccctc
     1021 tctacgatcg cgttgaaggc aagcatgaac tcgatatcgc tcttggtctt ttgaatgaca
     1081 ccagcggcat tgccgacgtt caccaatgtc ttcagcattc gaaccgacag gcgcgaatgc
     1141 aaaatgacga tcggctgctc ttcactgcga cggcatgcgg agagaagctc gagcaggatg
     1201 cgcttgttca tgtccggcat cagcacgtcg atgagcacga cctgcggctt caacacgcgt
     1261 acgagctgac gaacaccgga ggctcgcgaa aacacgctga cccggtgatt ctctcggttg
     1321 agtcgcgtgc tcacgtcccg aagcacgttc gggtcgtcat ctacgatcag gacatgcagg
     1381 ccaggcctga tgctgttggt tgtcttccgc gcagtcacga cgtccttcgc tgcgcaacga
     1441 aacggacgct agagcgcggt attaatgggg gaaaatcaac gtctcgcact tatccctttg
     1501 ggacaagatg acagctcgcc cccggattgg cgagcgagtc gtgcagcgga aatgacagaa
     1561 gcaagttcgg tgctagcgag ttccggaatt gggtcacccc cggtttgccg ccatttacgt
     1621 ggcaatccgc acggctacgg tcgcagcgcc gcaccgcgcg agcttcgctt tgagacgtcc
     1681 gcacgtcgca gtcgtgtcag tccgggacat cgcttgcccc tcgcatccag gaatttttcg
     1741 ggacgaattg actcagaatg ctaacttgta agacgggttt gccaatgccc gcccgcacac
     1801 atgctccgac cttggtcctg tcgctctgcc ttgcggcatg cagtgcgctg ggcggcggcg
     1861 acaccgggct gcgcgacgaa ttggccgcgt cgagggcaga ggcgcaagcc gaacacgagc
     1921 gggtccagga gctcgagcaa cggctcgccc agctcgagct caattcgaag gttcaggtgc
     1981 gagaccgccg catcgaggcg cgtctagagc gactcatcga ccagaacgaa gctctcttgc
     2041 gggctcagtc cgcgcccgct tgtcagcctt ccgctccgca gtccacgacc gaaccgagcc
     2101 ggcctccgac gggcacggcc tcgttcgcgg acgccaccga gggcgatcag aagaaacagc
     2161 tcgaagaacg gctgcgcgcc ttcgtacgcg gtcagggcgg cgggctttcg gccgagcaac
     2221 gcgaggccat gcgcgtcttg ctccgcaaag atcgggtgct cgacagggcc aacccctggc
     2281 acgacaactg agggccgcga atcagtagtc atacggcgcg cgcagtgccg cgcgcgaacg
     2341 tcacttgaac ggattctctt gcgacaatcc gtacgagctt gcgcggctgc gcggcgtcgt
     2401 gacgctgccg accggttgag cgctcggcgc tgctaccgcg ctcgccgccg cggctgcgct
     2461 cggcgcgatc gggacgacgc taaccgctcc tttaccgtgc accggtgccg cggcggaggg
     2521 cgacgccgaa gccaaaggcg acgccgacgg ctccacgcgc ggcgccgcat cttcgttcac
     2581 cacgggcacc accgccgtgt gcgtaggcac gttgaccgac ggcggttctg cggagctcgc
     2641 tgcctccgag cgcgtgcgcg cgtattgcgc gccgaccaca ccgaggccgg cgagagcgac
     2701 gaccgcgagc ggaatccaga gccgggacct cggcttcacg acgtccgaag gcggcgacgt
     2761 gaacacggcc ggagtcaacg cgggcattgg ccgcggcggc gcgatcggtg gctccgattg
     2821 cacgagcggc tgcgtttggc ccatgaaccc cggagcgagc gagctccgaa gccgcgaaac
     2881 ggcggacgag agctcgtctg ccgaagcaaa gcgctccgtg cgatcgctcg ccatcgcgcg
     2941 cgaaacgacc agatctacgg ccggcggaag cccattccgc acttcgctcg gcgtcgcgta
     3001 cttgccttga tatatcaaca ccgcgaggtg aggcagcgtc tccgcgacga acggcttgcg
     3061 cccagtgagg cactcgtaaa acagcacgcc gagcgcgtac acgtccgcgc gcgcgtcgat
     3121 gttcttctcg ccgcgcacct gctcgggcga catgtaaaag ggcgtcccga tcggtgagcc
     3181 ttcgagcgtg atcccgtcga cgccggtcgt cgccgaatcg aacttcgaaa cgccgaagtc
     3241 gaggatcttc acgaacgacg catccgaccc acacagaaac aaattctctg gcttcaagtc
     3301 gcggtgcacg atgcccgcgg cgtgcgcggc gctcaccgcg tcgcaagcct gaatcacgat
     3361 gtcgcaggcc gcgtcgagcg acaggggtcc gcgctgctcg agcacctccc cgagcgtgcg
     3421 cccggtgagc agctccatca cgatgtacgg ttcccccgtc tcgagccgac ccgcatcgaa
     3481 cgtctcgaca atgtgccgat tcccgatgcg ccccgccgcc gaagcctcgc gcaagaagcg
     3541 ctccaccacg ctcgggatgc cggccatctg cgggtgcagc agcttcagcg ctcgccgatg
     3601 gcgcgtgagc tcgtgctcca cctcgaacac cgcgcccatg ccgcccgccc cgagcagccg
     3661 cacaatccgc agcttgccag ccaggacctg ccccggtgcg aagcgttcgt tcatctccat
     3721 cgcgcgctca gcaggataac acggttccct ccgggcaacg gttgccgcca acccgtgtca
     3781 acccgagcga gtccacgcgc gctgcctcgt cgccaaaaag cccgcgttgc cgccgccgct
     3841 tgtcgagcca ccgcgcactc tacgccgttg gttggggcgc ccaccgaaaa gagcgcgcat
     3901 ggccgacgct gcgcggcaac gcgccagcgc cgcgcggccc tgaaaggttc cagggccgcg
     3961 cccggcgcac gccgtcgtga acgaccttca gccgccgctc ggcgccttcg ctacgcgcac
     4021 gatctgacca ctgccgccca cgccgtaaac gtacccgctg tccaccgcga acatcgaata
     4081 gaagtacggc gtcgcgttca cggtgccgat ctgcgtcggc gtgatgctgc cgtccttcac
     4141 gacgcgccag acggtcgcgt tctgatagta gtaatagaca tagttggcat cgacggcgag
     4201 acccgagctc ggcgccgcga tgccaatcgc aacgtcttgc accgcgtcgg gcgaaggcgc
     4261 cgcgctcacg gcgagacgac gaatcttgct gctcgagccg ttgtacgtgc tccagtacac
     4321 gtaactgccg tcggtcgcga tgccctggta gccgctgtag ccgctctgcg tggtcgaatc
     4381 caccgcgacg ggggcgccac cgttgaccaa cgaatacgcg tagacgacgt tcggtgcctc
     4441 gagccagtag aggtacccgc ccgcaacgac gaggtcgttc acggtgctac gcgcggtcgt
     4501 gagcccggag ggcggcgacg ctggctgcgc ggcggtcaag ctcttgcccg cggcgaggat
     4561 cacgttcgcg gcgttcacca tgtagagcgc cgtagcgttg ctggtaatga tcttggcgta
     4621 gtacgtgtcc ggcatcgtca ccgcggtttt cggcgtgctc gtctccatcg cggtgagcgg
     4681 cttccacagc acggtcgcgt tcggactacc cgagagcgag tagtaaacgt tcgcggcgtc
     4741 ggcgtgcagc ccgtagatgt agacgttgct ttggttcgtg acgagcgccg tgcccttcaa
     4801 agtgtcgggg gtggtcggca acgtcgcgac acggatctcg gtgccgctgc tcgtgttcac
     4861 ctttacgagc gtcgagcccg tgagcaccgc gctacccgtg taattgtacg tgagcgtggt
     4921 cgcgcccgtg ggatcgaaga cgatggccgc gccacattgg ccgttcgtgc acgtgccgcc
     4981 gccgcagtcc cgaccgcagg tgccgcagtt cttcggatcc gtcgccaacg tgacttcgca
     5041 gccgtcgacg ccgctcgtat tgcagtctgc gtagccctgc gtgcaagcgc ccatcacctt
     5101 gcaggtgagc gtcgctgcat cgcagaccga ggtgccgtgc accgaaccgc acgaaacgcc
     5161 gcaggcgcca cagctcgtcg ccaaggtgag gaacgtctca caatcggtcg gatccgtcgg
     5221 atcgcactcg ccctttccgg gtgggcactc gaacgggacg gtaccgccct cgccgccagc
     5281 gccgccggtc gtgacgacgg atccgcccgc acccggcgca ccaccgaaac cggcagtctt
     5341 gccgccggag cccgtcgacg tcttgccgcc gctcatctcg atcggcaggt cgccgccagc
     5401 gccgccgctc atctcgggcc cgccgccgga gctcgtcgcg ccacccttgc cggtcgcgcc
     5461 gcctttgttc gtgttgccgg tgccgccggt gccgccggta ccaccgctgc tgtcgacgaa
     5521 cggatccgga aggatgttgc ccgacgagtc gagcgtatcc ttcttcggat ccgtgctcgc
     5581 gcattgaccg ctcggtgcgc agacctggcc cggcgtgcag tcggtcgacg tcgtgcacgc
     5641 atcgcggcac tcaccatcga tgccgcacac ctgggtgccc ttgcaaacgg tcttgtcttt
     5701 ggtgcaatcc gtctcgactg cgagctgaca gacgaacccg gtcgcgccct ttacacagcg
     5761 ctcttcgccc gtcttgcagt cgcgatcctg agcgcactct tgatggcaac gcgcgaacgt
     5821 gcagacgagc gggtccgagc actccgaatt cagcgtgcag cctgccgaaa ggcccttggc
     5881 ctgaatcttc tccttgtcgc tgtcactgca cgcccaagcc gtaaggctga gcaccgagct
     5941 cagaaggatg attcgacccg gccgaagacc tagcgaaagc accatgaaaa acctccaatg
     6001 attgatcgct cgcgcataac acgcgctcaa tttttcgtct acaagcccag gcgctcagtg
     6061 atgtcttcgc gcagggcaac acgttaccgc gttcgcgttc gcgttttcga actctcgagt
     6121 cggtgagctc gaactcgagc ctctcgcaaa ctgacggcta gaggctacgg cctctcaccc
     6181 acgacccacg acccacgaac gccgctcaac aaaagggttc ctacagcggt ttggatttgg
     6241 tgagcgccgc agcaaaaacc cgctgcgagt gctcttcttc ccattcttct ggacgtttac
     6301 cgaacgcgcc gcgcgtctcg aaccgcag
//
LOCUS       DASTKS010001201         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1981245, whole genome shotgun sequence.
ACCESSION   DASTKS010001201 DASTKS010000000
VERSION     DASTKS010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981245"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFQ35_25600"
     CDS             complement(<1..802)
                     /locus_tag="VFQ35_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934113.1"
                     /translation="MLISSSARTVIPLFLAGALLGTPGLSSAAAPRGVTLRYEAPASC
                     PTRQEFHDAVTRRLGHSIDFDDETSGSVASVRIATAAGSATAEVSFESPGVAANLRQF
                     TTNSCGEAVEAAALVVALIIDPTIANAPSSATEPKPTEPGDAPAFRPPTAPKRAPSRE
                     ARHATAPTDAHSWVAHSRLSFGAVVAPTLGVSPSVLFAGGARFGFDPAPNDRGSWSPH
                     FALSALFARTGLIGAEADFGEITWFTSQLDGCSSKWPLTTTLTFRPCLS"
     gene            941..2704
                     /locus_tag="VFQ35_25605"
     CDS             941..2704
                     /locus_tag="VFQ35_25605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934114.1"
                     /translation="MGCSSRVPIGTGDGSGGASGGVASAGGQVSFGGSTVSAGGSDVG
                     GGGSLLETSSKLDLLFVIDNSFGMEHKLPRFRQAALSFLQRLIEPRCVGPAASVDVPV
                     GMPCPSGTTRERSPVRDLHVGVITTNLGAHGGQTCGESDDKAHLIATVRSGIESYSGL
                     GFAKWDPAQTASPPGLSSSSAFVLQVSNMLDGVGIGGCGYEASLESWYRFLVDPSPPE
                     RVIQQGEPTGLDTDLLAQRAAFLRPDSAVSIVVVSDENDCSIRDGDEGWLVTTQAFNS
                     NPFRMPKATSACQTDPNDRCCMSCAINPDLVPDGCSLPSDDVACRTGGPTHSGLEDSL
                     NLRCWDQKRRFGVDLLYPTTRYVGALTQPTVFDRALNPAPNPLFTSGANGTRGPKLIN
                     LTTLVGVPWQDLSVSPTGTLTFQNAEQLALNGRWNVVVGEGETPADPFMRESVAERDG
                     ANPITGDLVLPSTEVDPLGNAINGHELAFPNQDDLQNACTFALQTPVVCGEGSATCAC
                     SSARDYSALCQASASDPPGSTQYRTGAKPGLRQLRVQSQIGSSAVVGTICVDDAATLP
                     SESGYAPSFEALAAKMRQMLP"
     gene            2813..>3400
                     /locus_tag="VFQ35_25610"
     CDS             2813..>3400
                     /locus_tag="VFQ35_25610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934115.1"
                     /translation="MTPSDAFAWIAGMSSALGIFLSIRAFGLQQRGWLLVQAAVLAIV
                     AARVLSGREDLGYIAGGSWLLFVRAPALAAQRQHTLAMSYRFASAARWGRLAGILHPF
                     DGFAAQSQYLLAQGEIHAGNWRRAREMFEKLRSVREYRDLAQVELWRLDRDWARIIAF
                     VESHPELGSTSLVTSYLRALGETRQVDRMLGHYARL"
BASE COUNT          570 a         1073 c         1153 g          604 t
ORIGIN      
        1 cgctgaggca cggacgaaag gtgagcgtcg tggtcaacgg ccacttcgag gagcacccat
       61 cgagctgcga cgtgaaccac gtgatctcgc cgaagtcggc ctccgctcca atcagcccag
      121 tgcgtgcaaa gagggcgctc agggcgaaat gtggcgacca agatccgcga tcattgggcg
      181 ctggatcgaa accaaaacga gcgccgccgg cgaacagcac cgacggactc acgcccagcg
      241 tgggcgcgac gaccgcaccg aacgacagac gagagtgcgc gacccaagag tgcgcgtcgg
      301 tcggagctgt cgcgtggcgt gcttcgcgtg aaggcgctcg cttcggtgcg gtgggtgggc
      361 gaaatgcggg cgcgtcccca ggttccgtcg gcttcggttc ggtcgctgac gagggcgcat
      421 tggcgatggt gggatcgatg atgagcgcga cgacgagcgc ggccgcttcg acggcctcac
      481 cgcagctgtt ggtcgtgaat tgtcgcaggt tcgcggcgac gccgggtgac tcgaacgaca
      541 cttcggccgt tgcgctgccg gcagccgtcg cgatacgaac gcttgccacc gagccgctcg
      601 tctcatcgtc gaagtcgatc gaatgcccca aacgccgcgt caccgcatcg tggaactctt
      661 ggcgcgtcgg gcagctcgcg ggagcctcgt agcgcagcgt cacgcctcgc ggtgcggctg
      721 cggagctcag cccgggcgtc ccaagcagag ctccggcaag gaagaggggg atcacggttc
      781 gcgccgagct gctgatcaac atccggaggc ttggccatat agcaggagac tcgggcaatt
      841 cgcggctcgg gcgaggtgat tcatgcgcag tgcaatcggg cggttgttct ggggttcggg
      901 cgggttgatc ggatgcacgg ccctgagttt gtgggggccg atggggtgca gctcgcgagt
      961 tcccatcggc actggcgacg gttcgggagg cgcgtcggga ggcgtcgcca gtgccggagg
     1021 gcaggtgagc tttggcgggt cgacggtgag cgcaggcggc agcgacgtcg gtggcggggg
     1081 ctcgctgctc gagacatcga gcaagctcga cctcctcttc gtgatcgaca actcgtttgg
     1141 catggagcac aagttgccgc gcttccggca agcggcgctc tcgttcttgc agcgcttgat
     1201 cgagccgcgg tgcgtggggc ccgcggcgtc ggtcgacgtg cccgtcggca tgccttgccc
     1261 gagcggtacg acgcgcgaac gttcgccggt gcgtgacttg cacgttggag tgatcaccac
     1321 gaacctcgga gcacacggcg gtcaaacgtg cggggaatcg gatgataagg cgcatctcat
     1381 tgcaacggtg cgctccggaa tcgagagcta ctcggggctt ggcttcgcca agtgggatcc
     1441 ggcgcaaaca gcgagcccgc ccggcctctc gagctctagc gccttcgtct tacaagtgag
     1501 caacatgctc gatggagtcg gaatcggcgg gtgcggatac gaggcgagcc tcgaatcgtg
     1561 gtaccgcttt ttggtcgacc cttcgccgcc cgagcgcgtc atccaacaag gagagccgac
     1621 tggcctcgac accgatctgc tggcgcagcg ggccgctttc ctgcgccctg actccgctgt
     1681 cagcatcgtg gtggtcagcg acgagaacga ctgttcgatt cgggacggcg acgagggttg
     1741 gctggtcacg acgcaagcct tcaactccaa cccgttccga atgccgaaag cgacgtctgc
     1801 gtgccagacc gatccgaacg accgctgttg catgtcctgc gccatcaatc cggacttggt
     1861 cccggacggt tgctcacttc cttccgacga cgtggcttgc cgcacgggtg gaccgactca
     1921 ctccgggctc gaagactcgc tgaacctgcg ttgctgggac caaaagcgcc gctttggagt
     1981 cgatctcctc tatccgacga cccgctacgt gggcgcgctg acgcagccta ccgtgttcga
     2041 ccgagcgctg aaccccgcgc caaacccgct gttcacgagc ggggctaacg gcacgcgcgg
     2101 cccgaagctc atcaacctga cgactctggt cggcgtacct tggcaagatc tctccgtatc
     2161 gcccacggga acactgacgt tccaaaacgc ggagcagctc gcgctgaatg ggcgctggaa
     2221 cgtcgtcgtc ggtgaggggg aaacgccggc cgatccattc atgcgcgagt ccgtcgccga
     2281 gcgtgacggc gcgaacccaa tcaccgggga cctcgttttg ccgtcgacgg aggtcgaccc
     2341 actcggaaac gcaatcaacg gacacgagct ggctttccca aaccaggacg acttgcagaa
     2401 cgcctgcacc ttcgcgcttc agaccccggt cgtttgcggc gaaggatcgg ccacctgcgc
     2461 ttgttcctcc gcacgagatt acagcgcgct ctgtcaggca tcggcgagcg acccgccggg
     2521 ctcgacgcag taccgtacag gggcgaagcc ggggttgcgc cagcttcggg tgcagtcgca
     2581 aatcggttcg agcgcggtgg ttggtacgat ttgcgtagat gacgctgcga ccctgccgtc
     2641 cgaatcgggc tacgcgccct cgttcgaggc gctggccgcg aaaatgcgcc aaatgcttcc
     2701 ttgaagatct ccgcgacccg ggtggcaaag cgcgccccgg ttcctcgttc ggaacgagca
     2761 ccggggcgcg acacatcggc gggcagccga gcctctgcta gcgttgacgc ggatgacacc
     2821 ctcggatgct ttcgcctgga ttgccgggat gagcagcgcc ttgggcattt tcctgagcat
     2881 ccgcgccttc ggccttcagc agcgtggctg gttgttagtg caggccgcgg tgctcgcgat
     2941 agtcgccgcg cgggtgctct cggggcgcga ggatctcgga tacatcgcgg ggggctcctg
     3001 gctcttgttc gtccgcgcgc cagccttggc ggcccaacgt cagcacacgc tcgcgatgtc
     3061 gtatcgcttc gccagcgccg ctcgttgggg ccgccttgcc ggcattttgc atccattcga
     3121 tggatttgcg gcgcaatcgc agtacctgtt agcgcaaggc gagatccacg ccggcaactg
     3181 gcggcgcgct cgcgaaatgt tcgaaaagct tcgcagcgtg cgtgagtacc gtgaccttgc
     3241 gcaagtcgag ctctggcgtc tggatcgcga ttgggcgcgc atcatcgcgt tcgtcgaaag
     3301 ccacccggag ctcggtagca cgtcgctcgt gacgtcgtac cttcgagccc tcggcgagac
     3361 gcggcaagtc gatcggatgc tcggccacta tgcgcggttg
//
LOCUS       DASTKS010001202         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_638859, whole genome shotgun sequence.
ACCESSION   DASTKS010001202 DASTKS010000000
VERSION     DASTKS010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638859"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VFQ35_25615"
     CDS             complement(<1..1869)
                     /locus_tag="VFQ35_25615"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9934116.1"
                     /translation="MATASASATSDIHSEYGTTKYRRDASGYPSLSAPSLMAGTYALG
                     YLHSTDRLTQVTLTTLAARGALMSALSEHRLARLIDRSIRTLGLHVDVAEQVKQCDPE
                     TRALMDAYCRGFNAGAERRGVPWVLRLLGVDPLVCTPESIFSVFRFVTYFGLTSMQVS
                     TELIVAELAARGAPRRVFERLLGEKARGLDLKSLEGFQVPAELSYFGDARFGSPQAGS
                     NAFGVSKQRSATGGALLVGEFHMEVGRFPPLLYATHLGLPGGEYLSGITIPGLAWFAA
                     GRTRHVGWSYTYGHADNVDYLVERVKDERYWDGSEWQPLGRREEEVKIRNKPSEKWVF
                     YDNAYGRLMGDANVPNEALLANVRISGIGETYRAFSAARTVIDCRTVSELMDVQRSLR
                     SVSLEAILVDREGSIASIMTGQIDARPDDWTGAYPRRRTAPGTPPALDEEARPIVRNP
                     ASGFLASANQGGHGPNRSRWVTFPEPLYRFERIEELLAARERHDLGSLLAISYDTHDG
                     SAKGILSVWLPLMPEHPLREALRAFSSDASNPSHRETLSLYHTLHTEVCLEMLSEDVG
                     AAAAECFREWSGTALFQVQIDSVLRLEHPELLNEDGLRALLERAFPKAVATYLTQKQ"
     gene            complement(1967..2680)
                     /locus_tag="VFQ35_25620"
     CDS             complement(1967..2680)
                     /locus_tag="VFQ35_25620"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934117.1"
                     /translation="MGRRAEEWLPRLLSRRTIGRGLLGVAASFASRWSWAVEEREEKE
                     LLVVPLGEAPALEITVVEAALRAFYSVEVQVGERMALPRRAYYAKRRRYRAEKLLEAL
                     EEVSGQGRSGGKAFRVLGLASVDISTTKGGIEDWGILGLATIDGRVGVLSSYRCRRGA
                     KNAEQIAHRLGKTAVHEVGHTFGLEHCPNVGCVMEDGKGSVFTTDHEYDLCPTCRELL
                     GRANLLRVPRVAPPWPKPA"
     gene            complement(2680..3111)
                     /locus_tag="VFQ35_25625"
     CDS             complement(2680..3111)
                     /locus_tag="VFQ35_25625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934118.1"
                     /translation="MLRQSELRKLGVGLWVGAMSIRGVVGGASGFGAVGLGALGLGAL
                     GLGAVGCASTGDKLRARFAKERGCPESQVLVIPRGGVVYGAEGCGQEAEYVCGTFVSS
                     SMDKSAGSCEERNLPRQVRASDALPPPPRANDPEMPPGSSH"
BASE COUNT          692 a         1316 c          959 g          608 t
ORIGIN      
        1 ctgcttttgc gtgaggtacg tcgcaacggc tttcgggaag gcgcgttcga gcagcgcgcg
       61 caagccgtct tcattcagga gctcggggtg ctccaagcgc agaaccgaat cgatttgcac
      121 ctggaacaaa gccgtgcccg accactcgcg aaagcactcc gccgcggctg cgcccacgtc
      181 ttcgctgagc atttcgaggc aaacctccgt gtgcagcgtg tggtagagcg atagcgtctc
      241 gcgatggctg gggttgctcg cgtcgctcga gaatgcgcgc aaagcttcac gcaaggggtg
      301 ctccggcatg agcggcagcc acaccgacaa gatccctttc gcgcttccgt cgtgcgtgtc
      361 gtacgagatc gcgagcaatg agcccaagtc gtgtcgctcg cgcgctgcta gcagctcttc
      421 gatgcgctcg aatcggtaga gtggctccgg gaaagtcacc caacgcgaac ggtttgggcc
      481 gtggccgccc tggttcgccg acgccaagaa cccggacgcc ggattgcgca cgatcggtcg
      541 cgcttcttcg tcgagagccg gcggcgtgcc cggtgcggtt cgacgccgcg gatacgctcc
      601 agtccagtcg tcggggcgcg cgtcaatctg tcccgtcatg atgctcgcga tcgagccttc
      661 gcgatcgacc aagatcgctt cgagagacac gctgcggagc gagcgctgca cgtccatcag
      721 ctcgctcacg gtgcggcagt cgatcacggt gcgcgccgcc gaaaaggcgc ggtacgtctc
      781 gccaatcccc gaaattcgca cattcgcgag caatgcctcg ttcggcacgt tcgcatcccc
      841 catcaggcga ccgtatgcat tgtcgtaaaa cacccatttc tcactgggct tgttgcggat
      901 cttcacctct tcttcgcgtc gcccgagtgg ctgccactcg cttccgtccc agtaccgctc
      961 gtctttaacg cgctcgacca agtagtcgac gttgtccgcg tgcccatacg tatacgacca
     1021 gcccacgtga cgagtgcgcc cggccgcaaa ccacgcgagc cccgggatcg taatgccaga
     1081 caaatactcg ccgcccggca gccccaaatg agtcgcgtag agcaacggcg ggaagcgtcc
     1141 cacctccatg tgaaactcac ccaccaagag cgctccgccc gtcgccgagc gctgcttgga
     1201 aacaccgaac gcattcgaac ccgcctgcgg tgaaccaaag cgcgcatcgc cgaaatacga
     1261 gagctctgcc ggcacttgga aaccctcgag cgatttcaga tcgagcccgc gagccttttc
     1321 gccgagcagc cgctcgaata cacgccgggg cgctccacgc gccgcgagct cggcgacgat
     1381 gagttccgtc gaaacctgca tgctcgtaag gccaaagtaa gtcacgaagc ggaacaccga
     1441 aaaaatgctc tccggtgtgc acaccagagg gtccacgccg agcaagcgca gcacccaggg
     1501 cacccctcgt cgctccgctc ccgcgttgaa accgcgacaa tacgcgtcca tcaacgcacg
     1561 agtctcggga tcgcactgct tcacttgctc cgccacgtcg acgtgaagac ccaatgtgcg
     1621 gatgcttcga tcgatgagac gcgccaagcg gtgttccgaa agcgccgaca tcaacgcacc
     1681 ccgcgcggcc aatgtcgtca acgtcacctg agtcagccga tccgtcgaat gcaagtatcc
     1741 gagcgcgtac gtccccgcca tcaagctcgg cgcagacaac gacggatatc cagacgcgtc
     1801 ccttcggtac ttcgtcgtcc cgtactcgct gtggatatcg ctcgtcgcac tcgcactcgc
     1861 ggtcgccatc ggcatcgagg gcttatgccg tcgtcgcgtc ccgctgacaa cctctctccc
     1921 gcgccgagac gcctcctttc ggattcgcct cgcgcgcaaa cgtgcttcag gccggctttg
     1981 gccagggcgg cgccacgcgc ggaacccgta gaagattcgc acggccgagg agctcgcggc
     2041 acgtcgggca cagatcgtac tcgtgatccg tcgtgaacac gctgcccttc ccgtcctcca
     2101 ttacgcaacc gacgttcgga caatgttcca gaccaaacgt gtgccccacc tcgtgcacgg
     2161 cggttttacc caggcgatgc gcgatctgct ccgcgttctt cgctccacgt cgacaccgat
     2221 agctcgacag cacacccacc cgaccgtcga tcgtcgcgag ccccagaatg ccccaatctt
     2281 cgatgccgcc tttcgtcgtc gaaatgtcca cgctcgctag cccaagaact cgaaacgcct
     2341 tgccccctga gcgaccttgg ccgcttacct cttcgagcgc ctcgagcaac ttctcggctc
     2401 gatagcgcct ccgtttcgcg tagtacgcgc gccgcggcaa tgccatccgc tctccgacct
     2461 gcacttcgac cgaatagaag gcgcgcaacg ccgcctccac caccgtgatc tccagcgccg
     2521 gcgcctcgcc cagcggcacc accaacaatt ccttctcctc tcgctcctcg accgcccagc
     2581 tccagcgcga cgcgaacgaa gccgccaccc ccaacaaccc tcgcccgatc gtgcgacgac
     2641 tcagcaacct cggcagccac tcctcagcac ggcgccccat cagtggctac tcccgggcgg
     2701 catctcgggg tcgttcgctc ggggcggtgg tggcagcgcg tcactcgccc gcacttgccg
     2761 cggcagattg cgctcctcac agctgccggc tgacttatcc atgctcgagc tcacgaacgt
     2821 tccacaaacg tactccgctt cctgcccgca cccctcggcg ccgtacacca ccccgccgcg
     2881 aggaattacc aatacttgcg actccggaca acctcgctcc ttcgcgaatc gggctcgcaa
     2941 cttgtcaccc gtcgacgcac acccaaccgc gccaagccca agcgcgccaa gcccaagcgc
     3001 gccaagccca accgccccga acccactcgc gccgccgacc accccgcgga tactcattgc
     3061 acccacccac aggcccacac ccaactttcg caactcactc tgccgcagca tacggtgagt
     3121 ataagcgttg ggcgcgcaca tacccttctc gacagcgcaa gaacgagaat gttccgaacc
     3181 cccctgaggt cggactcgtc atcgccactt tcggcgccca cggtctcgaa actgcaccga
     3241 aaagcgctgc gtccctgccc gccgaccgca gcccgcttcg ctcgacccac gcctcccgag
     3301 caggtcacgc aagaaatctt tcgctagcat tcgaccttgc tcgatctcgc tcgaatcgag
     3361 gagccgctct ccaatgcgaa aaaacattgt gacaaaaaat gtcaaaacgt caaaatgctt
     3421 tggaacggct cgacctcggt ggccacgaag tgcgctggtt cgacgctccc cacgtgagcc
     3481 tggccaacac cgactgcggg aagcatcaag ggagcacctt cgactgaact caagccgacg
     3541 tttcggcccg ggggaaagcg gacgccgtca cccgc
//
LOCUS       DASTKS010001203         7205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_415037, whole genome shotgun sequence.
ACCESSION   DASTKS010001203 DASTKS010000000
VERSION     DASTKS010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415037"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFQ35_25630"
     CDS             complement(<1..442)
                     /locus_tag="VFQ35_25630"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET9934119.1"
                     /translation="MNRETVLSVRHWNEHLFSFTTTRDRGLRFKNGHFLMVGLEVEGK
                     PLMRAYSVVSPNYEEFLEFYSIKVPNGALTSRLQYVKPGDSVLVSRKPTGTLVQNHLR
                     PGKRLYLLATGTGLAPFMSMVRDPETYERYEHVVLAHGVRYASEL"
     gene            525..1418
                     /locus_tag="VFQ35_25635"
     CDS             525..1418
                     /locus_tag="VFQ35_25635"
                     /inference="COORDINATES: protein motif:HMM:NF008095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9934120.1"
                     /translation="MRYSLRQLEVFLAVAAGESVSRAARVLGMSQSAVSGSLAELERQ
                     FDIALFDRVGKRLRLNELGRSLRPSVEAVVSQANELEQRLGSKSESGALRVGATLTVG
                     NYLTAPLIAEFLRDDPSAKVSLKLENTAEIARAVLNFELDVALVEGELQDSELEVSAF
                     REDELVVFCAPEHPFARRRSLSDADLSSARWIVREPGSGTRQAFDHAMHGLLPELDIA
                     FELQETEAIKSAVKAGLGVGCISRIALDEPFRLKSLMPCRVPHRDFRRQFYSVVHRQK
                     HRSRAIRRWLTLCERRRRAAP"
     gene            complement(1479..2015)
                     /gene="ssuE"
                     /locus_tag="VFQ35_25640"
     CDS             complement(1479..2015)
                     /gene="ssuE"
                     /locus_tag="VFQ35_25640"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0044273 - sulfur compound catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET9934121.1"
                     /translation="MARVLTINGSPSSTSRTAALLGDVVRRLTADGHEVDALNVRELP
                     ARELLLAQADAPEIVAAAARVAAAEGVVIGTPIYKAAYSGLLKVFLDLLPQYAFERKT
                     ILPLATGGSVAHVLALDYGLRPVLSSLGARHVVGSYFVLDKTLRVMPDGLVELEPEIE
                     SKYRDALHAFTDSLARHA"
     gene            2214..2762
                     /locus_tag="VFQ35_25645"
     CDS             2214..2762
                     /locus_tag="VFQ35_25645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9934122.1"
                     /translation="MRVIVVGDGPGGLSAALFLAKNGQDVTVYGQDKTAMHWALLKNY
                     LGLPEIKGSDFQSVARAQVEALGARLVDARVESVKKVGEAFEVALENGERASSDFLIL
                     SEGKSPRLAQQLGLSFDEQRGLAVDINGKTALERVYAVGRLVRPTRSQAIISAGDGAA
                     AAIDILSTVQDKPVQDWDSPPE"
     gene            complement(2772..3086)
                     /locus_tag="VFQ35_25650"
     CDS             complement(2772..3086)
                     /locus_tag="VFQ35_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9934123.1"
                     /translation="MVQPISPRAAHSLLQEADLDVVDVREPSEWSTGHLPGARLVPLG
                     NLRRSPKSVLPRDGVIFVCAAGVRSETAARLALQHGLSNVYSLTGGTRGWSREGLPLV
                     RD"
     gene            3286..4047
                     /gene="tam"
                     /locus_tag="VFQ35_25655"
     CDS             3286..4047
                     /gene="tam"
                     /locus_tag="VFQ35_25655"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: protein motif:HMM:NF002463.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HET9934124.1"
                     /translation="MSQWDDRQYLKFADERTRAAIELLARVPLVTAEHVVDLGSGPGN
                     STALLAARFPSARVTGVENSAEMLARARRDYPSVEWVEMDLRRYRPERPVDVLFANAV
                     LQWVPDHATLFTALLEMVRPGGVLAVQMPKNFDEPSHRLMREIPGPFARRLQQVRDLT
                     PVQSAAYYYDLCAPFASSVDIWQTTYEHVMPDVASILEWVKGTGLRPYLDALTAAERN
                     VYLEAYAAALDDAYPPRVDKKRLFTFPRLFVVATR"
     gene            4190..4819
                     /locus_tag="VFQ35_25660"
     CDS             4190..4819
                     /locus_tag="VFQ35_25660"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9934125.1"
                     /translation="MIERHQKALRACTRCPDVFGPVVVGNPVASKVFLLGQAPGRREP
                     ELGRPFAWTAGKQLFKWFAPLGLDEERFRQRIYMAAVCRCFPGKATSGGGDRVPSRDE
                     VANCRPWIEAELELLEPELLILVGRLAIEQFLPPRPLVEQIGRVHSLTWRGRSLDVIP
                     LPHPSGASTWPRTEPGKSLTARALVLISKHPAFARLLSIESTQRRKSSR"
     gene            complement(4848..5786)
                     /locus_tag="VFQ35_25665"
     CDS             complement(4848..5786)
                     /locus_tag="VFQ35_25665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934126.1"
                     /translation="MRRDRIARLSWGLWVGVALYTSSCSGPPDDLFAPRGGAGGAIAK
                     GGTAGTSGSGESGSVTGGVGGEGGFDDGFGGAPSSGGAPFIEGGRVGSGGVTLGTGGT
                     SGKGGSGGTSAGAANGGKAGMGNLPNEIVLQINRNSDDCTWILVNGAYQERLRFSEAN
                     PWLEVGSDTEQGRIGLRFALPIPPKATIVAASLQLTRVDGNAAATNTLTVQVFDTGDV
                     PAFDASHTHGPRDHGTAGLYGTAVRGTMVGEKGKTITTGDLKVLVQRVVDRADFVQDS
                     GTIGFVIFPDTVRDWAAYGDSSNGMGASLRVSYRVP"
     gene            complement(5798..6841)
                     /locus_tag="VFQ35_25670"
     CDS             complement(5798..6841)
                     /locus_tag="VFQ35_25670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934127.1"
                     /translation="MASSSAPAVHASAPPRHSVHGFKETLRLNRERLRDELSKLLRRN
                     KALLSESAAFVDARREKLPAGLRKPLERVGSTTLVTGFLGLSGALLLFGVLALVLRSG
                     SGASAHPRLAVTRNSAVPSAEPRAQAPESATTAVAETKTVTPAASTRTSANVPSAGVA
                     PRDEEAMLLELANTYTSERRDAEAVALVQRVLLRRPELKSDPRVSKLLMRTARSDDRT
                     ASEQSFGLLEGTLGETGAEAIYDLWRERDASDRVHRRAESFLKSSGFDRVSSTALYSA
                     VKLRLARTCEQKHALLRLAGDVGQKRTLEYLRELEQRTTCAAGQAECYACLGADSLLK
                     QTIERVAERVGQK"
BASE COUNT         1188 a         2269 c         2435 g         1313 t
ORIGIN      
        1 cgagctcgct cgcgtagcgc acgccgtggg cgagcacgac gtgctcgtag cgttcgtaag
       61 tctccggatc gcggaccatg ctcatgaacg gcgcgagccc ggtccccgtc gcgaggaggt
      121 acagccgctt gccgggccgc aggtggtttt gcaccaacgt gccggttggt ttgcgcgaga
      181 ccagcaccga gtcgccgggc tttacgtact gcagccggga ggtgagggcg ccgttcggca
      241 ctttgatgct gtaaaactcg aggaactctt cgtagttcgg gctcacgacg ctgtacgcgc
      301 gcatgagcgg cttgccctcg acctccagcc ctaccatcaa gaagtggccg ttcttgaagc
      361 ggagcccgcg gtcacgcgtg gtcgtaaagc tgaacaagtg ttcgttccaa tgacgaacgc
      421 tgaggacagt ttctcgattc aagtggttca cgagcctttt gttagctccg cttgcttaat
      481 cggtaaaacg ggataatccg gaatggtaat tcggggaatc cgatatgaga tacagcttgc
      541 gacagctcga ggtctttctt gcggtagcgg cgggcgagag cgtcagccgc gcggcgcggg
      601 tgctcggcat gtctcagtcg gccgtgagcg gctcgctcgc cgaactcgag cgtcaattcg
      661 acatcgcgct cttcgaccgg gtcggcaaac gtctgcgttt gaacgagctc ggccgctcgc
      721 ttcgccctag cgtggaggcg gtagtttcgc aggcgaacga gctcgagcag cgcctcggaa
      781 gcaagagtga atccggcgcg cttcgggtag gcgccaccct gaccgtgggc aactacctga
      841 ccgctccgct catcgccgag ttcctccgtg acgacccatc cgcaaaggtc agcttgaagc
      901 tcgagaatac cgccgagatt gcgcgcgcgg tcttgaattt cgagctcgac gtcgcgctcg
      961 tggaaggaga gctccaagat agtgaactcg aggtgtcagc gtttcgcgaa gacgagctgg
     1021 tcgtcttctg cgctccggaa catcccttcg cgcggcggcg cagcttgagc gacgcagacc
     1081 tctcgtccgc tcgatggatc gtccgcgagc cgggatccgg gacgcgacaa gctttcgatc
     1141 acgccatgca cgggctgctg ccggagctcg atatcgcctt cgagctgcaa gagaccgaag
     1201 cgatcaagag cgccgtaaag gcggggctcg gcgtcgggtg catctctcgc atcgctttgg
     1261 acgagccatt tcggttgaag tcgctcatgc cctgccgagt gccacaccga gactttcgtc
     1321 gccaattcta ctcggtggtg caccgacaaa agcaccggag ccgcgcgatt cgccgctggc
     1381 tgacgctctg cgagcgacgc cgtcgcgctg cgccttgacg gctcgtcgag ctcggcgtcg
     1441 aaggcagcgg tcagagatgc gcgcgcgggc tcgtcgcttc acgcgtgacg cgcgaggctg
     1501 tcggtgaagg catgcagggc gtcgcggtac ttgctctcga tttcgggctc gagctcgacg
     1561 agcccgtctg gcattacgcg cagtgtcttg tcgaggacga agtagctgcc gacgacgtgg
     1621 cgagcaccga gcgagctcag aaccggtcga aggccgtagt cgagcgcgag cacgtgagcg
     1681 acgctgcccc ccgtcgcgag cggtaggatc gtctttcgtt cgaaagcgta ctgcggcagc
     1741 agatccaaaa acaccttcag gagcccgctg tacgccgcct tgtaaatcgg cgtgccaatg
     1801 accacgcctt cggcagccgc gacgcgcgcc gccgcggcta caatctcggg tgcgtcggct
     1861 tgcgcgagca agagctctcg ggcgggaagc tctcgaacgt tcagagcgtc gacttcatgc
     1921 ccgtccgcgg ttagccggcg tacgacgtcg cctagcagcg ccgcggttcg cgaggtcgaa
     1981 gaggggctgc cgttgatggt gagcactcta gccatgattc gagggaacgt cgtcgtcgct
     2041 gaaaagagct tggcgtggtg atacgaagtc tttcaaacaa agccgcttcg gacgaatatg
     2101 tcaaggatcg agcctctgcg agcgccgtgc ctgcgccttt ttggctcgct cgcgcggcgc
     2161 ttgacgccgg ttcgaaagtg cttcacttgc gtcatctcaa acgaggtgat gccatgcgag
     2221 tgatcgtagt cggtgacggg cccggcggcc tcagcgctgc tctctttttg gcgaagaatg
     2281 ggcaagatgt cacggtgtat ggccaagaca agaccgccat gcactgggcc ctcctgaaga
     2341 actacctcgg gctgcccgag atcaagggca gcgattttca gagcgtcgcc cgcgcgcagg
     2401 tggaagcgct cggggcgcgc ctcgtcgacg ctcgcgtcga gtcggtgaag aaggtgggcg
     2461 aagcgttcga agtagcgctc gagaacggtg agcgcgcgtc ttcggacttc ttgattctat
     2521 cggagggcaa atcgccacgg cttgctcagc agcttgggct gagcttcgac gagcagcgcg
     2581 ggctcgcggt cgacatcaac ggcaagaccg cgctcgagcg cgtctacgcg gtgggccgtt
     2641 tggtgcgccc gactcgcagt caggccatca tcagcgctgg tgacggggcc gcggctgcga
     2701 tcgacattct gtccaccgtt caggacaagc cggtacagga ctgggactcg ccgcctgagt
     2761 gagccgcgcg ctcaatcgcg gacgaggggc aaaccctcgc gcgaccaccc tcgagtgccg
     2821 ccggtgaggc tgtacacgtt cgaaaggccg tgctgtagag cgagtcgcgc cgcggtctcg
     2881 ctacggacgc cagcagcgca gacgaatatc acgccgtcgc gcggcagcac actcttgggg
     2941 cttcgccgca gattcccgag cggcaccagg cgcgcgccgg gcaagtgccc cgtcgaccac
     3001 tcgctcggct cccgaacgtc gaccacgtcc aagtcagctt cctgaagcag cgaatgtgcg
     3061 gcgcgggggc taattggttg gaccatgcgt ccattatagg agacgaaatc tcgtgggcaa
     3121 gcccgctgaa gcctgcgcga catctttcct gactttttgc agcctgcccg gcgtggcggt
     3181 taccgcgccc tccactacag cggacagaac tagccggcgt tttcgcgcgg gcaggtatcg
     3241 tccattggga gtaaactttc cggctcagag ggagaagaga ccacgatgag ccagtgggac
     3301 gaccgccaat atctgaaatt tgcggacgaa cggacccgtg cagccatcga gctgcttgcg
     3361 cgcgtgccgc tcgtgactgc cgagcacgtc gtcgacttgg gctcggggcc cggcaactcg
     3421 accgcgctct tggcggcgcg ctttccgtcc gcgcgcgtca ccggcgtcga gaattcggcc
     3481 gaaatgctgg cgcgagcaag gcgcgactac ccgtcggtgg agtgggtaga gatggatctg
     3541 cgccgctacc gcccggagcg gccggtggac gtcttattcg ccaacgcggt gttgcagtgg
     3601 gttcccgacc acgcgacgtt gttcacggcg ctgctcgaga tggtgcgtcc cggcggcgtg
     3661 ctcgcggttc agatgccgaa gaacttcgac gagccgagcc atcgcctcat gcgcgagatc
     3721 cctgggccct tcgcgcggcg ccttcaacag gtgcgcgacc tcacccccgt gcagagcgca
     3781 gcttactact acgacctctg cgctcccttc gcgagctcgg tggacatctg gcaaacgacg
     3841 tacgagcacg tgatgcccga tgttgcgtcg attcttgagt gggtgaaggg cacagggctt
     3901 cgcccttatc tcgacgcttt gaccgccgcc gaacgcaacg tgtacttgga ggcttatgcg
     3961 gcggcgctcg atgacgcgta ccctccgcgc gtcgacaaga agcggctgtt cacgtttcct
     4021 cgcttgttcg tggtcgcgac gcgctaaggg cccgcgaatg ggtaagtcat cctgtgcgag
     4081 ccgatcctct tgccgcggcg gacaggctcg tcgtcgcgct catccggcta ggtggctcgg
     4141 ttgcttgcgt ggtgtgaggt gctagcgaaa gcgaattcgc cggcttccgt tgattgaacg
     4201 acaccagaag gcgctcaggg cctgcacgcg ttgcccggac gtgttcggcc cggtcgtggt
     4261 cggaaaccca gtcgcttcca aagtcttctt gctcgggcaa gcgccgggac ggcgtgagcc
     4321 agagctcggg cgaccgtttg cttggacggc agggaagcag ctgttcaagt ggtttgcgcc
     4381 gctcggcctc gacgaggagc ggtttcggca gcgcatctac atggccgcag tctgtcggtg
     4441 ctttccggga aaagcgacga gcggtggcgg cgatcgggtg ccttcgcggg atgaagtcgc
     4501 gaactgtcgg ccatggatcg aagccgagct cgagctgctc gagcctgagc tcctgatctt
     4561 ggtgggtcgg ctcgcgatcg agcaattcct cccgcctcga cccctcgtcg agcagatcgg
     4621 cagagtgcat tcgctgacgt ggcgaggtcg ctccctcgat gtgatcccgc tgccgcaccc
     4681 ctctggcgca tcgacttggc ctcggaccga gccgggtaag tcactcacgg cgcgagcgct
     4741 ggtattgatc tcgaagcatc cggcgttcgc gcgattgcta tcgattgaat cgactcagcg
     4801 acggaagagt tcgcgctagc gctgccggcg aagctgcgcg caacgcctca aggcacgcga
     4861 tacgaaacgc ggagcgatgc gcccatgccg ttcgagctgt cgccataagc cgcccagtcg
     4921 cgtacggtgt ctggaaaaat cacgaaacca atcgtgccag agtcttgcac gaagtccgcc
     4981 cgatcgacca cgcgctgcac gagcaccttc agatcgccgg tcgtgatggt cttgcccttt
     5041 tcgccgacca tggtcccgcg cacggccgtg ccatagaggc ccgccgtgcc gtggtcgcgc
     5101 gggccgtgcg tgtgactcgc gtcgaaggcc ggcacgtcgc cggtgtcgaa gacctggacg
     5161 gtgagagtgt tcgtggccgc ggcgttcccg tcgacgcgcg tcagctgcag ggacgccgcg
     5221 acgatggtgg ctttcggagg aatcggaagc gcaaagcgca ggccgatgcg cccctgttcg
     5281 gtgtcgctcc cgacctcgag ccacggattc gcctcggaaa agcgaagtcg ctcttgatac
     5341 gctccgttca cgaggatcca ggtgcagtca tcgctgtttc gattgatctg caagacgatc
     5401 tcgttcggca gatttcccat gccggctttg ccgccgttag ctgcaccagc gctcgtgccg
     5461 cccgagccgc ctttgcccga ggtgccgccg gtgcccaagg tgacgccgcc cgaaccgact
     5521 cgcccgcctt cgatgaaggg cgcgccgccg ctcgacgggg ccccgccgaa gccgtcatcg
     5581 aaaccgccct cgccgccgac acccccggtg actgagccgc tctcgccgct gccgctcgtg
     5641 ccggcggtgc cgccctttgc gattgctccg ccagccccac cccgtggcgc gaacagatcg
     5701 tctggggggc ctgagcaact cgacgtgtac agcgcgactc cgacccaaag cccccaactc
     5761 agtcgcgcaa ttcggtctcg tctcacaagc tcaccttcta cttctgtccg acccgttccg
     5821 cgacccgctc gatcgtttgc ttgaggaggg agtcagcgcc gaggcaggca tagcactcgg
     5881 cctgcccagc ggcgcaagtg gtgcgttgct cgagctcacg taaatactcg agcgtccttt
     5941 tctgcccgac gtctcccgcc agccgcaaga gcgcgtgttt ttgctcacac gtgcgagcta
     6001 ggcgtagttt gacggccgaa tagagcgccg tcgacgatac tcggtcgaac ccgctgcttt
     6061 tgaggaagct ttcggcccgc cggtgcacgc ggtcgctcgc gtcacgttcg cgccacaagt
     6121 cgtaaatggc ttccgcgccg gtctcgccca gcgttccctc gagcaaccca aagctttgct
     6181 cggaggcggt tcggtcatcg gagcgagcgg tgcgcatgag gagcttggag acgcgcgggt
     6241 cgctcttgag ctcgggacgg cggaggagca cgcgctgcac cagcgccact gcttcggcgt
     6301 cgcgtcgctc gctcgtgtac gtgttggcga gctcgagcag catcgcttct tcgtctcgcg
     6361 gagctacccc cgcggacggc acgtttgccg acgttctggt cgaagcggct ggcgtcacgg
     6421 ttttcgtctc cgcgaccgcc gtcgtcgcgc tttcaggtgc ctgggctcga ggttcagccg
     6481 aggggacggc ggagtttcgc gtcacggcca ggcgcggatg tgccgacgcg ccggaaccgc
     6541 tgcgcaaaac gagcgcgagc acgccgaaca aaagcagcgc gcccgaaagc ccgaggaagc
     6601 cggtgacgag cgtggtcgag ccaacgcgct cgaggggctt tcgaaggccc gcggggagtt
     6661 tctcgcgccg agcgtcgacg aacgccgccg attcgctgag cagagctttg ttgcggcgca
     6721 ggagcttcga gagctcgtcg cgcagacgct ctcggtttag acggagagtc tccttgaagc
     6781 catgaacgga gtgccgtggg ggagccgagg cgtggaccgc gggcgcagaa gacgaagcca
     6841 tgttgccaac ggctagagca gcgctcggag caggcggcgc ggcgctaggc gcgggagggg
     6901 cgtggctgtc cgcgagggcg gcgcttggtg cgagtggcgc ggcgctcgga gaagggggaa
     6961 cggcgcttgg cactggcggt gccgcacttg gggcgggcgg tgctgtgctc acggcgacgg
     7021 gtgcgtgaga ggccgcgtgg cgcggaggcg gaggtggcgc ggagtgagcc gcgtggttga
     7081 gcgcgctcgg tgggggcggc gcgtggctcg caccattcgt gtgcaccgcg ggagacaccg
     7141 cgtgattcgc ggcgtcgcgg cgtggcgggg gcggcggccc tttgggcccg tccgagttcg
     7201 tgcct
//
LOCUS       DASTKS010001204         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1981793, whole genome shotgun sequence.
ACCESSION   DASTKS010001204 DASTKS010000000
VERSION     DASTKS010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981793"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..771)
                     /locus_tag="VFQ35_25675"
     CDS             complement(70..771)
                     /locus_tag="VFQ35_25675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934128.1"
                     /translation="MTATKKAKKSSAQKRERKERRFTPEPTYASRITTYVGMAGALAL
                     GAGVYGQWIGEPPVAVAPVLLAAGSIAFLGALWKGSSEVGALRVGDAGVALETNDELT
                     RIFWCDIERVSLNAGRVEVRGKNATIAFPAEAHHKGLAWLLSEGGRRVPDVLAVKRAD
                     IESLPEPKEFDGEQVAVEELQVTGRHCRATDKPISFERDARLCPNCGETYLKDHVPKK
                     CLTCQQELGNRAREL"
     gene            1165..4056
                     /locus_tag="VFQ35_25680"
     CDS             1165..4056
                     /locus_tag="VFQ35_25680"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET9934129.1"
                     /translation="MGTSPRVALASREATLSLVLGTRARDHTAGFEGVAPLSVVPRLP
                     FPPGAAGVLCELAASTLSPVVSLSRAMCEELLLPLCREGWLDGVRDGDVTNARRLRAS
                     HGKPWSVRLEARLPEGEGEAAIEFSGRVVRSAPLGEETLAPTRIDALLEVGYVVSGDE
                     LGVLDADCVAWVRSLREGPVVVPRAEVGEFMGALLALPRGPRVTNVEDIGYSLRAGKP
                     EPCLRLAPSVGNERLLRGTLVFRYAKGQGGLHASGDGVASETDEQAARWELGCDMRPQ
                     TLVDGEQGLLVQRDYAEECAVIKRLGAAGFGVTVWEAETPDCPFDVRVHFDDFARACE
                     VLMDEGVRVELDGSLLRRARGFRAFVSSEQDWFELKGNARFDRNALELPELLDQVRQK
                     NRFARLGDGSLGLLPERWIAEFAPLASLVNPKHERLRFHALQALLIDGLLGSWSPAAD
                     PSFAKMRERVQAFNGIEPLTEPAGFKGELRSYQRVALGWLAALDELGLSGCLADDMGL
                     GKTVVVLAFLWLRAARAPRGGRPSLLVVPKSLLFNWRSEARAFTPELKLLVHHGAARL
                     PPGEHFEQYDVVLTSYGTLRRDAAALAALTFDYVVLDEAHAIKNQESLVHRSARRLSS
                     RGRLALTGTPVENHLLELWALLNYLNPGVLERFEHLVRTFERARPSEIAVTEVIQKLA
                     RPFVLRRTKDEVAPELPPRIEKTLYVPLSGAQAVVYRELADHYRARMLERREQRKYIG
                     SSMRGVSHHNGHESARALEALLRLRQVACHPALLAGSDVDLESPAARAATAAGASSAK
                     LDLLLEQLGALGEEGHKSLVFSQFTGFLKIVREALRERGIAFEYLDGETQNRQSVVER
                     FQRREGACVFLISLKTGGTGLNLTAADYVFILDPWWNPAAETQAVDRAHRIGQTRPVI
                     AYRLLTKGTVEAKVAALQANKRALTHAIFSDDAAFSAKLTQKDLEYLLS"
     gene            4117..>5264
                     /locus_tag="VFQ35_25685"
     CDS             4117..>5264
                     /locus_tag="VFQ35_25685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9934130.1"
                     /translation="MRWLTTLVPIATLLAVAFLVLVVGAPRPVASVRLLGGPTDGLAR
                     FSGLLLAEVDGAPLSTARLELFARSEAGSPARSVVTLDGDGMGETSLDFAKVPRSFSL
                     SVTRDGRELARGRVQLSRETWLARAQRRGGYHGGAASGELEIVIAPGRGVFAVPFGAP
                     LFLRVKREGRALAGAELELRSDGAKVNPSRVTTNAEGIARVELTPLEHVVTLQARATA
                     GEHSGTLAATIPIAPGALFVRRTTEGLRVSSPVPRDVAYLTLLNESGRVRGLRVSLQA
                     TPEGAESELVSANDLPDGPLWARAASEPFATGTSAVGWPLTDAVDAPAKTLDVRDVLL
                     LDSSAAATQIERLRRRRVLGWAVVVGLVGALTSIATVLLRARRSGRAL"
BASE COUNT          786 a         1650 c         1880 g          948 t
ORIGIN      
        1 cgcaagcttc tgcgggctgg ctcgaagtgg atgtcgacct gccctccacg gcgaacgccc
       61 cgggtgggct cagagttcgc gcgcgcggtt accgagctct tgctggcagg tgaggcactt
      121 cttcggcacg tggtctttca agtacgtctc gccgcagttc gggcacagac gggcatcgcg
      181 ctcgaaggag attggcttgt ccgtcgcgcg gcagtgccgc ccggtgacct gcagttcttc
      241 gacggcgacc tgctctccgt cgaactcttt gggctcgggg agggactcga tgtcggcgcg
      301 tttgacggcg aggacatcgg ggacgcggcg gcccccttcg ctcaacagcc acgcgagccc
      361 tttgtgatgc gcttcggcgg ggaaggcgat ggtggcgttc tttcccctca cttccacgcg
      421 acctgcgttc aggctgacgc gctcgatatc gcaccagaag atgcgcgtga gttcgtcgtt
      481 ggtctcgagc gcgaccccag cgtcaccgac gcgcagggcc ccgacctcgc tgctcccctt
      541 ccacaaggcg ccgaggaacg cgatgctgcc cgccgcgagc aagacgggcg cgacggcaac
      601 gggaggctcg ccgatccatt gaccgtagac gcccgccccg agcgcgagtg cgccggccat
      661 gccgacgtag gtcgtgatgc gcgaggcgta agtgggctcc ggggtgaagc gccgttcttt
      721 tcgctcgcgc ttttgagcgc tgctcttctt ggccttttta gtagcggtca ttgctgaagc
      781 ggcgagaagg tacaccgaat tgccgtcgcc tgcgaagcgg cttgggaggg cagcgtaccc
      841 tacaggtcga gatgaactgg aggcaaggtc gagacgcaac cggggcaggt gggaagaaca
      901 ggaaggcgcc taaaaaccag gcagcactgg ggttcattcc cgggccacgc gtttggtcgc
      961 caagcgcggc aaaagtgcgc tagcacgctc cggatgacgc gctcggaccc gccacgaagg
     1021 aagacgggcc cgggtgccgg gacggggacg ggcaccgcca cgacggcgcg gtcgtggcgg
     1081 gcactcacac gggcagagtc gcatccggcg ttctcggagc ggccacgcga gagcgcgctg
     1141 tgctttttgg tcgatctcgc gtccgtgggt acgagtccgc gcgtcgccct ggcgagtcgc
     1201 gaggcgacgc tgagcctcgt gctgggtacg cgggcgcgcg atcacacggc tgggttcgaa
     1261 ggtgtcgcgc cgctttcggt cgtgccgcgc ttgccgtttc cgccaggcgc ggccggcgtg
     1321 ctgtgtgagc tcgcggcgag cacgttgtcg ccggtggtct cgttgtcgcg cgcgatgtgt
     1381 gaagagctgt tgctgccgct ctgtcgcgaa ggttggctcg acggagtgcg ggacggagac
     1441 gtgacgaacg cacggcggct ccgcgcctcg catgggaagc cgtggagtgt gcgcttggag
     1501 gcgaggcttc cagaaggcga gggcgaagct gcgatcgagt tcagcgggcg cgtcgtgcgc
     1561 tcggcgccgc tcggcgaaga gacgctcgcg ccgactcgca tcgacgcgct gctcgaggtc
     1621 ggttacgtcg tctcgggaga cgagctcggc gtgctcgacg ccgactgcgt tgcttgggtg
     1681 aggagcttgc gtgaagggcc cgtcgtggtg ccgcgcgcgg aggtaggaga gttcatgggg
     1741 gcgctgctgg cgctgccccg tggtccgcgc gtcacgaatg tcgaagatat cgggtattcg
     1801 ctgcgagcgg ggaagcccga gccgtgcttg cgcctagcgc cttcggttgg caacgagcga
     1861 ctcttgcgcg gaactttggt gtttcgttat gcgaagggtc aggggggcct ccacgcgagc
     1921 ggtgacggag tcgcgagcga aaccgacgag caggcggcgc ggtgggagct cggctgcgat
     1981 atgcgaccgc aaacgctggt cgatggggag cagggacttc tggtccagcg cgactacgcg
     2041 gaggagtgcg cggtcatcaa gcgcctggga gcagccggct tcggggtgac ggtgtgggag
     2101 gcggagacgc cggattgccc tttcgacgtg cgggttcact tcgatgattt cgcgcgcgcg
     2161 tgtgaggttt tgatggacga gggggtccgc gtcgagctcg acggaagtct gctgcggcgc
     2221 gctcgcgggt tccgcgcgtt cgtgagcagc gaacaagatt ggttcgagct caagggcaac
     2281 gctcgcttcg atcgcaacgc cctggagttg ccggagctgc tcgatcaggt gcgacagaag
     2341 aaccggttcg cacgcctcgg cgatggaagc ctcgggctct tgccggagcg atggatcgca
     2401 gagtttgctc cgctcgcgtc gctcgtgaac ccgaaacacg agcggctccg cttccacgcg
     2461 ctgcaagcgc tgttgatcga tgggctactc ggctcgtggt cgccggccgc agatcccagc
     2521 tttgcgaaga tgcgtgagcg cgtgcaagcg ttcaacggaa tcgagccgct gaccgagcct
     2581 gcaggattca aaggggagct caggtcttac cagcgcgtcg cgctcggctg gctcgccgcg
     2641 ctcgacgaac tcggcttgag tggttgcttg gcggacgaca tggggctcgg caagacggtg
     2701 gtcgtgctcg cctttctctg gcttcgggcc gctcgcgcgc cgcgtggcgg tcgcccttcg
     2761 ttgctcgtcg tgccgaagtc gttgctcttc aattggcgct cggaggcgcg cgccttcact
     2821 ccggagctga agctcctggt gcatcacggc gctgcgcggc tcccgcccgg cgagcacttc
     2881 gagcagtacg acgtcgtctt gacgagctat ggaactctgc ggcgcgacgc ggcggcgctg
     2941 gcggcgctca cgttcgacta cgtggtcctc gacgaagcac acgccatcaa gaatcaagaa
     3001 agcttggtgc accgttcagc gcgacgtctc tcgagccgtg gtcgcttggc gctcaccggg
     3061 acaccggtcg aaaatcacct gctcgagctc tgggccttgc tgaactacct gaaccccgga
     3121 gtgctcgagc gcttcgagca cctggtgcgc actttcgagc gtgcgcggcc gagcgagatt
     3181 gcagtgacgg aggtgatcca aaagctcgcg cggccgttcg tgctgcggcg caccaaggac
     3241 gaggtcgcgc ccgagctgcc gccgcgcatc gaaaagacgc tgtacgttcc gctcagcggc
     3301 gcacaggcgg tcgtttaccg cgagctcgcc gaccactatc gcgcgcgcat gctcgagcgg
     3361 cgcgagcagc gcaagtacat cgggagctcg atgcgcggcg tttcgcatca caacgggcac
     3421 gagtcggcgc gggcgctcga ggcgctacta cgactgcgcc aagtggcctg ccaccctgcg
     3481 ctcctcgccg ggagcgacgt ggacctcgag tcgcccgcgg ctcgcgcggc gacggcggcg
     3541 ggcgcgtcga gcgcgaagct cgatctgcta ctcgagcagc tcggggcgct cggagaggaa
     3601 gggcacaagt cgctcgtgtt ctctcagttc actggcttct tgaagatagt gcgcgaagcg
     3661 ctgcgagagc gcggcatcgc tttcgaatac ctggacgggg aaactcaaaa tcggcagagc
     3721 gtggtcgagc ggtttcaacg tcgggaaggc gcgtgcgtct ttctcatcag cctgaaaacg
     3781 ggcggcactg gtttgaatct caccgctgcg gactatgtat tcatactcga cccctggtgg
     3841 aaccccgcgg ccgagacgca agcggtcgac cgcgcgcatc gtattggcca gacgcgcccc
     3901 gtcatcgcct accgcttact gacgaagggc accgtcgagg caaaggtcgc cgcgctccag
     3961 gcgaacaagc gggcactgac ccacgccatc ttctcggacg acgcggcctt ttcagcgaag
     4021 ttgacgcaaa aagacctgga gtacttgctg tcgtaggcgg tttcttctgc ggtttggtcc
     4081 acggttcgcg tttcgcctgt atcctgcttt cagcgaatgc gctggctaac aaccctcgtc
     4141 cccatcgcca cgctccttgc ggtcgcgttt ctcgttctcg tggtcggcgc gcctcgcccc
     4201 gtcgcatcgg tgcgtttact cggcgggccc accgatgggc tcgctcgctt cagcggtctc
     4261 ttgctcgccg aggtcgacgg ggcgccgctt tcgaccgcgc ggctcgagct cttcgcgcgc
     4321 tccgaggccg gatcacccgc gcgcagcgtg gtgacgctcg acggcgatgg gatgggcgaa
     4381 acgagtctcg acttcgccaa ggtacctcgt tccttttcgt tgtcggtcac gcgagacggt
     4441 cgcgaactcg cgcgagggcg cgtgcagctt tcgcgagaga cttggctcgc gcgtgcccag
     4501 cgtcgcggcg gctatcatgg cggcgccgcc tccggagagc tcgaaatcgt gatcgcgccg
     4561 ggtcgcggcg ttttcgcggt gcccttcggg gcgcctctgt ttttgcgcgt caagcgagag
     4621 gggcgtgctc tcgccggcgc ggaactcgag cttcgttcgg atggcgcgaa ggtgaatccg
     4681 agccgcgtca ccaccaacgc ggaagggatc gcgcgggtcg agctcacacc gctcgagcac
     4741 gtggttacgc ttcaagcgcg cgcgacggcg ggagagcatt cgggcacatt ggccgccacg
     4801 attccgatcg ctcccggcgc gctgttcgtg cgtcgcacga ccgaagggct gcgcgtgagc
     4861 tcgccggtgc cgcgcgatgt cgcgtatctc accctcctga acgaatccgg gcgtgtgcgt
     4921 gggctgcgcg tctcgttgca ggcgacacca gaaggcgccg agagcgagct cgtctccgcg
     4981 aacgacttgc cggacgggcc gctctgggcc cgcgccgcga gcgagccatt tgcgacgggg
     5041 acttcggcgg tggggtggcc gctgacggac gccgtcgatg cgcccgccaa aaccctcgac
     5101 gtgcgtgatg tgctgctgct cgattcgagc gcggccgcca cgcaaatcga gcgcttgcgg
     5161 cgccggcgtg tgctcgggtg ggcggtggtc gtagggctgg tcggcgcgct cacgagcatc
     5221 gcgaccgtgc tcttacgagc gcgtcggagt gggcgtgctc tgga
//
LOCUS       DASTKS010001205         6051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_415445, whole genome shotgun sequence.
ACCESSION   DASTKS010001205 DASTKS010000000
VERSION     DASTKS010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415445"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFQ35_25690"
     CDS             <1..416
                     /locus_tag="VFQ35_25690"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET9934131.1"
                     /translation="CRKKYGEAVYIVGFGTDHGTVAAASDWDEPMEIMQVRPARPDSY
                     ERICHDTALPAFSCALRHPRREALRDELAEARLERAIGVVYLPDAERQSHYFHAVLPK
                     QFDEYVWFDETRAVTPIDGPVLHVGDAPETYPFGV"
     gene            504..2450
                     /locus_tag="VFQ35_25695"
     CDS             504..2450
                     /locus_tag="VFQ35_25695"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9934132.1"
                     /translation="MLIRGCNSLEISARLVPWALALALVGGCKGSIDDGDQAASGGDT
                     SNVSGGGSSANPTGSGGVNTSGNSGRPGATSGGNQGVGGAGGGAMGGASTATDPGMFG
                     ACPSGEPEPGATPLMKLSTLQYRNTVRDLLAASGLSDMNAELAPLLSAVPDDSTVQFR
                     GLDQRISSDHLMAYFKVATAIGDGATKSAARLTALAGACATQSTLAASCLDGFLNSFG
                     RRALRRPLNSDELAEYRDIAQGSSPKATNPAEALRNVVVTLLMSPRFVNHLELDGSPM
                     AGREDYIALDPFVVASRLSYTFWQTLPDEPLLTAAADGTLATDAGFRKQLDRVFADAR
                     TQQTVWQFWNEWLHFESFTGFVSDRPAFQSLAKGEQVGVAGHDHYRDMVQEVRDLTEL
                     YTWKQAGTFNDLMTSTVSVTKSADLAHLYGVPAWSGSGAYPTFTDGSRVGLLQRAALL
                     ASSLETTNPFHRGSLIRRAVLCDDLPRPDPNSLPPGSLDTPPPSSALTTRQRYEAKVS
                     GNNLCKGCHSAFSNIGYVLEAYDALGRFRTSEKVFDEQTGALVATLPIDTNASIDLTG
                     AGASVVSGPAELNQRILASGKVEACLSANYFRYALRRDPQGDSADSCAFENMRASLSK
                     GNLSEVFRSVALEASFRRKKVGTP"
     gene            2447..4066
                     /locus_tag="VFQ35_25700"
     CDS             2447..4066
                     /locus_tag="VFQ35_25700"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9934133.1"
                     /translation="MNQRNSKMETFMPSRRAVLRGAAGALLALPFLESLAPRQAQGQT
                     MTPPKRFIVLKSFSTQLVKEWYPAFTGNGYALKNSKYSGSKADGTTLLTQKLVSGKNY
                     TWAPLRDLKTDTGISGILGASLNPFLDKLTLIRGLDFLPSVNHNYGGLLGNFSSCTAA
                     TPCDADSLADVPTIDQVIAYSSKFYQGTPGVRSLHISQGVTDSMSYTNLGMLSGAVQQ
                     VKARTNPLDAFNDVFGTGAPSTGAPSPDRDKLLIDRVYQDYVKLKKSSRLSVADKQLL
                     DQYTTLIAELQAKLTTTPTMACTAPATPQSLANNTGLDTKDITTKWNLFLDVVAAAIM
                     CDRTRIITIGVHKALGPGPDSTDSKLAGFYHSEDASGGTWHGLAHDFSNENSRRMLKG
                     INQWIANEVFAKLLTKLDVPEANGSTYLDNSLVYWGNELGFNHIAYSVPCLLAGSAGG
                     FIKPGRYLDYIDWDGRSYFSQEDGNVIKGIPHNQFLVTALQAMGLQPADYERDGKPGY
                     GSTSVNGRASDTWPVDYDLATVGQVLPGIRG"
     gene            4271..5179
                     /locus_tag="VFQ35_25705"
     CDS             4271..5179
                     /locus_tag="VFQ35_25705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9934134.1"
                     /translation="MSNARMLIGIAALSLASCVSQGKYDAAVADANAAKQRAEQERTR
                     GQEKDAKIAQLNAALSALQQAQAVRDERLAKLTTDGEQLHKELDDATALNQSLRDELT
                     KLGKNADKLLAERASVASALEQTKARLEELRKAQAKAEARAALYREVALKFKKMVDAG
                     ALAVSLREGRMVLVLANDVLFDSGKTELKPAGRATLDQVAEVLTSLRMRRYQIAGHTD
                     NQPIHVSPFDSNWELSAARGLVVLQYLIKKGMPPSALSAAGYGEYDPVAANTSTEGMA
                     KNRRIEIVLQPNIDEIVSVPGVAESP"
     gene            5290..5781
                     /locus_tag="VFQ35_25710"
     CDS             5290..5781
                     /locus_tag="VFQ35_25710"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET9934135.1"
                     /translation="MPCRRRSQSFVLALFLSCACASRPDDSCGPESAVVERVIDGDTV
                     ELASGERIRYLLVDTPENTNGHVECFGPEAFERNRELVEGRTVQLRYDVECRDRYGRA
                     LAYVSVGSREVNSRLISEGFACVLHVPPNGDDRAHEFQSLEAAARQQKLGLWGACSEI
                     PCN"
BASE COUNT         1135 a         2010 c         1887 g         1019 t
ORIGIN      
        1 tttgccgcaa gaagtacggc gaagcggtgt acatcgtcgg gttcgggacg gatcacggaa
       61 ccgtcgccgc agcttccgac tgggacgagc cgatggagat catgcaggtt cgccccgcgc
      121 gccccgatag ctacgagcga atctgccacg acaccgcgct gccggcattc agctgcgcgt
      181 tacgccaccc aaggcgcgag gcgctgcgtg acgagctcgc cgaagcacgg ctcgagcgcg
      241 cgatcggcgt tgtgtacttg ccggacgcgg agcggcagag ccactatttt cacgccgtgc
      301 tgccgaagca gttcgacgag tacgtctggt tcgacgagac gcgagcggtc acgcccatcg
      361 acggacccgt cctacatgta ggcgacgcgc ccgagacgta cccgttcgga gtgtgatggg
      421 taagtggccg gaatcgcgca attaatgatg gcgttctagt tttgttggac cgactgggcg
      481 tcctcacggg tcgtaccccg cggatgctaa ttcgcggatg caactcgcta gagatttcgg
      541 cgcgactcgt cccctgggct ctcgcgttgg cgctcgtcgg tggctgcaag ggcagcatcg
      601 acgatggtga ccaagcggcg tcgggcgggg acacgagcaa cgtgagcggg ggcggctcca
      661 gcgccaatcc cactggcagc ggtggcgtga acacgagcgg caactccggg cgacctggcg
      721 caacctcggg cggaaaccag ggcgtcggag gcgccggtgg gggagccatg ggcggcgcgt
      781 ccaccgccac ggatcccggc atgttcggcg cttgcccctc gggtgagccg gagccgggcg
      841 cgacgccgct gatgaagctt tcgaccctgc agtatcgcaa cacggtgcgc gacctgctcg
      901 cagcgagcgg cctcagcgac atgaacgccg aactcgcgcc cctgctctct gccgttccgg
      961 acgacagcac cgtgcaattc cgagggctcg accagcgaat ctcgtcggat cacttgatgg
     1021 cctacttcaa agtcgcgacc gcgatcggcg acggcgcgac caagagcgcg gcgaggctca
     1081 ccgcgctcgc cggcgcttgc gcaacccaaa gcactctcgc cgcgagttgc ctcgacggct
     1141 tcttgaacag cttcggtcgg cgagcgctgc gtcgcccgct caacagcgac gagctcgccg
     1201 aataccgcga catcgcgcag gggtcgtcac ccaaagccac caatccagcg gaagcgctgc
     1261 gaaatgtggt cgtcacgctc ttgatgtcac cgcgcttcgt gaaccacctc gagctcgacg
     1321 gctcgcccat ggcgggacgt gaagactaca tcgctctcga ccccttcgtc gtcgcctcgc
     1381 gtttgtcgta cacattttgg caaactctgc ccgacgagcc gctgctcacc gccgccgccg
     1441 acgggacgct cgcgaccgac gccggattta gaaagcagct cgatcgagtg ttcgccgacg
     1501 cgcgcaccca gcaaacggtt tggcagtttt ggaacgaatg gctgcacttc gagtcgttca
     1561 cggggttcgt ctcggatcgt cctgcgttcc aatcgcttgc caaaggcgag caagtcggcg
     1621 tcgccggcca cgaccactac cgcgacatgg tgcaggaggt gcgcgatctg acggagcttt
     1681 acacctggaa gcaagccggc accttcaacg acctgatgac gtccaccgtt tcggtgacca
     1741 aatcggccga tctcgcgcac ctgtacggtg tcccggcttg gagcggtagc ggcgcgtacc
     1801 caacgttcac cgacgggagc cgcgtcgggc tcttgcagcg cgcggcgctg ctcgcctcga
     1861 gcctcgagac gacgaacccg tttcaccgcg gctcactcat ccgccgcgcc gttctttgcg
     1921 acgacttacc gcggccggat cccaattcgc ttccgcccgg ctcgctcgac acgccgccac
     1981 cgagctcggc tctcaccacc cgacaacggt acgaggccaa ggtgagcgga aacaacctgt
     2041 gcaaagggtg ccactctgct ttcagcaaca ttggctacgt gctcgaggcg tacgatgcgc
     2101 tcggtcgctt ccgcacgagc gagaaggtct tcgacgagca aaccggtgcg ctcgtggcga
     2161 cgctgcccat cgacacgaac gcctcgatcg acctgaccgg cgccggagca tcggtcgtga
     2221 gcggcccggc cgagctgaac cagcgcatcc tagcgagcgg aaaagtggag gcgtgccttt
     2281 cggccaacta cttcagatac gcgttgcgac gcgatccgca gggcgatagc gcggactcgt
     2341 gcgctttcga aaacatgcgc gcgtcgctct cgaaaggaaa cctctccgag gtgtttcgca
     2401 gcgttgcgct cgaggcgagc tttcgccgga aaaaggtggg cacgccatga accaacgaaa
     2461 cagcaagatg gaaacgttca tgccttcacg acgcgcggtc ttgcgcggtg cggccggtgc
     2521 gctgctcgcg ctgcccttct tggagtcgct cgctccacgc caggcccagg gccagacgat
     2581 gacgccaccg aagcgcttca tcgtactcaa atcgttcagc acgcagctag tgaaagagtg
     2641 gtacccggct ttcaccggca acggctatgc cctgaagaac agcaagtact cggggagtaa
     2701 ggccgacggt acgaccctgc tcacgcaaaa gctcgtctcc ggtaagaact acacgtgggc
     2761 tccgcttcgt gatctcaaga ccgacaccgg aatctcgggc atcctcggcg cttcgctgaa
     2821 cccgttcctc gacaagctga cgttgattcg cggtctcgat ttcttgccgt ccgtgaatca
     2881 caactacgga gggctgctcg gcaatttttc gtcgtgtacc gcggcgaccc cgtgcgacgc
     2941 cgatagcctc gccgacgtgc ccaccatcga tcaggtgatc gcctactcgt cgaagttcta
     3001 tcaggggact ccgggggtgc gctcgctgca catctcgcag ggtgtcactg attcgatgtc
     3061 gtacacgaac ctcgggatgc tgagcggcgc tgtccagcaa gtcaaagcgc gcacgaaccc
     3121 gctcgatgcc ttcaacgacg tgttcggcac cggggcgccg agcaccggcg cgccttcgcc
     3181 cgaccgagac aagctgctca tcgaccgcgt ttatcaagac tacgtaaagc tgaaaaagag
     3241 ctcgcgcctt tcggtcgcgg acaagcagct gctcgaccag tacacgacgc tcattgccga
     3301 gctccaagcg aagctcacga ccacgccgac catggcgtgc accgcgccag cgacgccgca
     3361 gtccctcgcg aacaacaccg gcctcgacac gaaggacatc acgaccaagt ggaacttgtt
     3421 cttggatgtg gtggccgccg cgatcatgtg cgaccgcacc cgcatcatca ccatcggcgt
     3481 tcacaaggcg ctcggaccgg gcccggactc gaccgactcg aagctcgccg gcttctatca
     3541 ttccgaagac gcgagcgggg gcacatggca cggccttgcc cacgacttca gcaacgaaaa
     3601 ctcgcggcgc atgctgaagg gcatcaacca atggatcgcg aacgaggtct tcgcaaagct
     3661 cttgaccaag ctcgacgtcc ccgaggcaaa cggcagcact tacctcgaca attcgttggt
     3721 ctactggggc aacgagctcg gtttcaatca catcgcctat agcgtgccgt gcctgctcgc
     3781 cggcagcgcc ggaggcttca tcaagccggg acgctatctc gattacatcg actgggacgg
     3841 ccgttcgtac ttctcccaag aggacggcaa cgtcatcaag ggcatcccgc acaaccaatt
     3901 cttggtgacg gcgctgcagg cgatggggct ccagcccgcc gattacgagc gcgatggtaa
     3961 gcctggttac ggctcgacga gcgtcaacgg gcgtgcctcc gatacgtggc cggtggacta
     4021 cgacctcgcg accgtcgggc aggtgttgcc cggcatccgc ggatagactg cgcggcgtcc
     4081 tcgaccacct gcggcacgtc gcaccagaac ggcccgcgaa gggctactct gtgaatgagg
     4141 cacgcagtgt ttgcgctccc ttcgtgctcg cgcgcaagcg ctgctgctgc gccaacggga
     4201 agagaccgcg agttggcgag cgcgccgagc gtacgcatcg ggcacacatc gtgcacgttg
     4261 gttcgtcatc atgtcgaacg cacgcatgct cattggcatc gctgcgctgt ccctcgcgag
     4321 ctgcgtctcg caaggcaagt acgacgcagc cgtagccgac gcgaacgcag caaagcaacg
     4381 cgccgagcaa gaacgaacgc gcgggcaaga aaaggacgcg aagattgcgc agttgaacgc
     4441 ggcgctcagc gcgctccagc aggcgcaggc ggttcgcgac gagcggctag cgaagctcac
     4501 gacggacggc gagcaacttc acaaggagct cgacgacgcg accgcgctga accagtcgtt
     4561 gcgcgacgag ctcaccaagc tcggcaagaa cgcggacaag ttgctcgcgg aaagggcatc
     4621 cgtcgcctcg gcgctcgagc aaaccaaggc gcgcctggaa gagctccgta aggctcaggc
     4681 aaaagccgag gcgcgcgcgg cgctctatcg cgaggtcgct ctgaagttca aaaaaatggt
     4741 cgacgcgggc gcgctcgccg tctcgctacg cgaaggtcgc atggtgctgg tgctcgccaa
     4801 cgacgtgctc ttcgactccg gcaagaccga gctcaagcct gctgggcgag cgacgctcga
     4861 tcaagttgca gaggtactca cgagcctgcg catgcggcgc tatcaaatcg ccggccacac
     4921 cgacaaccaa ccgattcacg tatcgccctt cgattcgaac tgggagctgt cggccgcgcg
     4981 cggtctcgtc gtgctccagt acctgatcaa gaagggcatg ccgccgtcgg cgttgtcggc
     5041 cgcgggttac ggcgagtacg atccggttgc cgcgaacacc tcgacggagg gcatggcgaa
     5101 gaaccgccga atcgaaatcg ttctgcagcc gaacatcgac gaaatcgtgt cggtgccggg
     5161 cgtcgccgag agtccatagc gcggtccaac gcgcccccga caactgctca ggacccacga
     5221 aagcgacgcc gccggcgctc gcagcgccgt tctacgcaac tggccccgac tgcgcgccga
     5281 tgagcggaca tgccatgccg ccgtcggtca cagtcgttcg tgctcgccct gtttctttcg
     5341 tgcgcctgtg cctcgcgtcc cgacgactct tgcggtccgg aaagcgccgt cgtcgagcgc
     5401 gtcatcgacg gcgacacggt cgagctcgcg agcggcgagc gaattcgcta cctcctcgtc
     5461 gacacgccgg agaacaccaa cggccacgtg gagtgctttg ggcccgaagc cttcgagcgg
     5521 aaccgcgagc tcgtcgaagg tcgaacggtc cagctgcgtt acgacgtcga gtgccgcgat
     5581 cgttatggcc gagcactggc ctacgtgtcg gtcggttcgc gcgaagtgaa ttcgcggctc
     5641 atttcggagg gcttcgcgtg cgtgctccac gttccgccga acggtgacga ccgcgcgcac
     5701 gaatttcagt cgctcgaggc ggccgctcgc caacagaagc tcggactctg gggcgcatgc
     5761 tccgaaatcc cctgcaattg aaggtgtgga tgcccccacc gtgcagtgct cgccgcttcg
     5821 cggctgcgcg cttcccgctc gccgagtagg gacccccgtt tacacggggg cccctctcac
     5881 agaaccggac ttgtgggcct cacatccggc tcttcgggat gccgacgttc catcgggtcg
     5941 tacgggcaag gacgcatcat tgtcgccgtg cgccgtccga ttgtgcgggg agttcggcat
     6001 cggtttgcac catcgctatc ccgctgagtg cgggccgttc cagattcggt c
//
LOCUS       DASTKS010001206         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_415686, whole genome shotgun sequence.
ACCESSION   DASTKS010001206 DASTKS010000000
VERSION     DASTKS010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415686"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFQ35_25715"
     CDS             complement(<1..960)
                     /locus_tag="VFQ35_25715"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9934136.1"
                     /translation="MTDLPTEWPNFHGMPDDDALTASRPRGQKLASWIWWDGEIREAS
                     APNVHYYANALHYGTAVFEGIRCYPSAQGPRLVRLRDHLERMLLSARMYGMKVPFDLD
                     SLARGAIEVTRRNGIQNGYLRPLAYFGAGPIDLKPKRECAVNAFIAVRELGTFLGPDA
                     LRNGIRVMISSWRRPHHTVLPTMAKASGHYANSVLAAHEAMDRGFDDAILLAQDGNVS
                     CATGHNVFFVKGGTLITNDESSSIVPGITRDCVLTIARENNVPVQIRSFTREELIRAD
                     EVFLTGTAAEVTPVRELDGALFATGKNTLGALFQRAYLRAVTGR"
     gene            1005..2165
                     /locus_tag="VFQ35_25720"
     CDS             1005..2165
                     /locus_tag="VFQ35_25720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9934137.1"
                     /translation="MFSSRSARDREPNELTRELEALCRSGKALWDLTQSNPTRAGIRY
                     DEDGILTALGSPAALSYQPHPLGPRAAREAVAHCWASEGVAVSADHVVLTASTSEAYG
                     ALFKLLCDPGDEVLIPAPSYPLFEHLASFESVDVRNYPLGFDTGWYVDVDAVAARIGP
                     RTRAIVLVSPNNPTGNYVTSDELARLERLGVPLISDEVFATFPIDAPADRARSVLDAK
                     QVLCFALSGLSKLAALPQMKVAWTAVGGPPALADEALARLELILDAYLSPSTPSLGAL
                     PRLLETRHVAAEMLHARVRRNARHLKERARDTATTLLPIQGGWYAVLRLPATESEEDW
                     VLGLLRHEQVLVQPGYFYDFAREPYAVVSLLTPEATLDEGIERLLRYVERRC"
     gene            complement(2168..2998)
                     /locus_tag="VFQ35_25725"
     CDS             complement(2168..2998)
                     /locus_tag="VFQ35_25725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9934138.1"
                     /translation="MTSLVLWSRSRAFVTSLMALCVLLFSQAAFAAGRIEWKSKDLQE
                     REGGSWRLEVAIYLPRAPDVAHVPMKFEFQPEAYYERAMMDGDKLVERVVPLENRQAL
                     IESVDVGFLDAGSGKTESRTRFTFKVTRAHGYEAGEYKVTIRDGRNGQVIGTPQVLKF
                     KGENEIIDRRAMVFSGEKKKKKKDDAEGGEKKADSGDEKKAEEKKADEPSEKPAAAEE
                     PSEKEDSGSNGAEDETIKKKPGGCGCRLEASGAENHGTFALALLTGAVVVARRRRRAA
                     "
     gene            complement(3529..>4135)
                     /locus_tag="VFQ35_25730"
     CDS             complement(3529..>4135)
                     /locus_tag="VFQ35_25730"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET9934139.1"
                     /translation="ELECIVGVRPPERKRRQRVRLDVSLFVDLSPAGRSGRITRTIDY
                     ARVADELQNMLRFREYRLMESAAEELSAMLFAAHPPLQAVELCIEKPEALRGRATGTG
                     AVRVRRDRESFPITRRQIDGGFVDLVLETHEATLSLVTLDAAKEFSSVARACVVWLTH
                     GDVGGTRSQMDLGEPIDCSTPIVAGPNGATLFVCQIREDVL"
BASE COUNT          671 a         1381 c         1308 g          775 t
ORIGIN      
        1 gcgaccggtg acggctcgca ggtaagcgcg ttgaaacagc gctcccaagg tatttttgcc
       61 ggtcgcgaag agcgcgccgt cgagctcgcg tacgggggtg acttccgccg ccgttcccgt
      121 caaaaaaact tcgtcggcgc ggatgagctc ttcgcgcgta aagcttcgga tctgcaccgg
      181 tacgttgttc tctcgcgcaa tggtgagcac gcagtcgcgc gtgatgccgg ggacgatgct
      241 cgagctctcg tcgttggtga tcagcgtgcc gcccttgacg aagaagacgt tgtgccccgt
      301 ggcgcacgag acgtttccgt cttgcgcgag gaggatcgcg tcgtcgaaac cacgatccat
      361 cgcctcgtgc gcggcgagca cgctgttcgc gtaatgtccg ctcgcttttg ccatggtcgg
      421 caatacggtg tggtgcggac gacgccagct cgaaatcatc acgcgaatac cgtttcgcag
      481 cgcgtccggc ccgaggaaag tgccgagctc gcgcacggca atgaaggcgt tcaccgcgca
      541 ctcgcgcttc ggcttcaaat cgatgggccc agcgccgaag taggcgaggg gacgcaggta
      601 tccgttttga ataccgttgc gacgcgtcac ttcgatcgcg ccgcgcgcga gtgaatcgag
      661 gtcgaaaggg accttcatgc cgtacatgcg cgcggagagg agcatgcgct cgaggtgatc
      721 gcgtagacgc acgagccttg gtccttgcgc gctcggataa cagcggatgc cctcgaatac
      781 ggccgtgccg tagtgcaggg cgttcgcgta ataatgaacg ttgggcgcgc tagcctcgcg
      841 aatttctccg tcccaccaaa tccaggaggc gagcttttgg ccccgtgggc gcgacgcggt
      901 gagcgcgtcg tcgtcgggca taccgtgaaa attcggccac tcagtgggaa ggtcggtcat
      961 ggcgggtccg tgttcggagc agtataagcc ggaaccttcg ccgtatgttt tcctcacgtt
     1021 cggcacgcga tcgagagccc aatgagctca cccgggagct cgaggcgctt tgccgctctg
     1081 gaaaagccct ctgggacctc acccaatcga atccgacgcg ggccggcatt cgctacgacg
     1141 aagacggaat cctgacggcg ctcggcagtc ccgccgccct ttcgtaccag ccgcatccgc
     1201 tcggccctcg cgccgcgcgc gaagccgtgg cccactgctg ggccagcgaa ggcgtcgcag
     1261 tctcggcgga ccacgtcgta ctcaccgcga gcaccagcga agcctacggc gcgctcttca
     1321 agctgctctg cgatcccggc gacgaagtcc tgattcccgc gccgagttac ccgttgttcg
     1381 agcacctcgc gtctttcgaa tcggtcgacg ttcgcaacta tcccctcggc ttcgataccg
     1441 gctggtacgt cgacgtcgac gcggtcgccg cgcgcattgg tccgcggacg cgcgccatcg
     1501 tgctcgtgag ccccaacaat ccgaccggta actacgtgac gtcggacgag ctcgctcggc
     1561 tcgagcgcct cggcgtgccg ctcatctccg acgaagtgtt cgcgaccttt cccatcgacg
     1621 cgcccgctga cagagctcgg agcgtgctcg atgcaaagca ggtgctctgc tttgcgctgt
     1681 cgggcttgtc gaagctcgcg gcgctgccgc aaatgaaggt ggcttggacc gcggtgggtg
     1741 ggccgcccgc gctcgccgac gaggcgttgg cgcggctcga gctgatcctc gacgcctacc
     1801 tttcgccgag cacgccttcg ctcggcgcgc tcccgcgact cctcgagacg cggcacgtcg
     1861 cggctgaaat gctgcacgca cgcgtacgcc gcaatgctcg ccatttgaag gagcgcgcgc
     1921 gcgacaccgc aacgacgctg ctcccgatcc aaggcggctg gtacgccgtg ttacggctgc
     1981 cggcgaccga gagcgaagag gattgggtgc tcggcctttt gcggcacgaa caggtgctcg
     2041 ttcagcctgg ctacttctac gacttcgcgc gcgagcctta cgcggtcgtg agtctgctca
     2101 cgcccgaagc gacgctcgac gaaggcatcg agcgtctgct tcgttacgta gagcgacgct
     2161 gctgagctca ggccgcgcga cggcgacgcc gcgcaacgac gaccgcgccc gtgaggagcg
     2221 cgagcgcgaa ggtaccgtga ttttcggctc cactcgcttc gagacggcaa ccgcaaccac
     2281 ccggcttctt cttgatggtc tcgtcttcgg cgccgttcga gccgctgtcc tctttctcgg
     2341 agggctcttc ggcggcggcc ggcttttcgc tcggctcgtc tgccttcttt tcttcggcct
     2401 tcttctcgtc gcccgagtct gccttctttt cgccgccctc ggcgtcgtcc tttttcttct
     2461 tctttttctc gccgctgaac accatcgcgc ggcgatcgat gatctcgttc tcgcccttga
     2521 acttgagtac ttgcggagtg ccgatgacct gaccgttgcg gccgtcgcgg atggtcacct
     2581 tgtactcgcc ggcttcgtac ccgtgagcgc gagtcacctt gaaggtgaag cgggtgcggc
     2641 tctcggtctt gccgctgccg gcgtcgagga acccgacgtc gacgctttcg atcagcgcct
     2701 ggcggttctc gagcggcacg acgcgctcca ccagcttgtc cccgtccatc atggcccgct
     2761 cgtagtaggc ctctggctgg aactcgaact tcatcggaac gtgagcgacg tccggcgccc
     2821 gggggagata gatggccact tcgaggcgcc acgaccctcc ctcgcgctct tgcaggtctt
     2881 tgctcttcca ctcgatacga cccgccgcga aggcggcttg agagaacaag aggacgcaaa
     2941 gcgccatcaa cgacgtgacg aatgcgcgcg aacgactcca aaggacaagg cttgtcaacg
     3001 aaacctccgg cgcgcgacgc taacaccgga gcgcgcgttc cgtcgaccgt ccgcggcatc
     3061 taacgcccaa gctcgggcgg gccagcgaac gaggcggctc gggcgattgc gccgcgtcag
     3121 ctcgtctcgg agccgtcgtc cagccacgtc ggtgctggaa ttgtgtcgtg gcgctcgaag
     3181 tcgacctccg tcggctccac tggctccggg cgaggcgtca cggcgccgcg cgggcccgtg
     3241 cccgaacgct gcgggcggct gcgccgggca ggagcatcgc caggagtagg tgtcgaggca
     3301 gtgtttggct tgctggcgtt catggtgccc tcagggtgcc gctcgtcctc tcacgcgcga
     3361 cgcccccttt ccgtgagagc gcttatcaca aatgcgcccc gatcgcgaaa cggtaaacaa
     3421 attgactatc gagagcgcat cacaatcgcg agtgatgttc tcgcgttaga tcgtacccct
     3481 cgaggttggt aattcgcttt tcgctgcagc gcgccatgag cgagcgcgtc agagaacgtc
     3541 ttcgcgaatt tggcacacga acaacgtcgc tccgttcggc cccgccacga tcggcgtcga
     3601 gcagtcgatg ggctcgccga gatccatttg cgagcgggtg ccgccgacgt cgccgtgggt
     3661 cagccacacc acgcacgcgc gcgcgacgga cgagaactcc ttcgccgcgt ccagcgtcac
     3721 gagcgacaac gtcgcttcgt gcgtctcgag cacgagatcg acgaacccgc cgtcgatttg
     3781 acgacgcgtg atcggaaagc tctcgcggtc acgccggacg cgcacggcgc ccgttcccgt
     3841 cgctcgcccg cgcagcgctt ctggcttttc gatgcaaagc tcgaccgcct ggagcggcgg
     3901 gtgcgctgcg aacagcatcg cagagagctc ctctgccgcg gactccatca agcggtattc
     3961 gcgaaagcgc agcatgttct gcaattcgtc cgcgacgcgc gcgtagtcga ttgtccgcgt
     4021 gatgcgcccg gagcgccccg cagggctcaa gtccacgaat agcgagacat cgaggcggac
     4081 acgctggcga cgcttgcgct ccggcggacg aactccaacg atgcactcga gctcc
//
LOCUS       DASTKS010001207         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_639613, whole genome shotgun sequence.
ACCESSION   DASTKS010001207 DASTKS010000000
VERSION     DASTKS010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639613"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..364)
                     /locus_tag="VFQ35_25735"
     CDS             complement(74..364)
                     /locus_tag="VFQ35_25735"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9934140.1"
                     /translation="MIRLSVEGSRWRASGSLGADACASFRELLLLAESAAPAGEPREL
                     DLAGVESIDVKGVQVLAAFVTTSRSHRLCDASDSVKSLLTRLGALSLLDTTP"
     gene            complement(361..2337)
                     /locus_tag="VFQ35_25740"
     CDS             complement(361..2337)
                     /locus_tag="VFQ35_25740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9934141.1"
                     /translation="MTIDQLVVTFHRETEELLAALEEGLLELEHTDDPAACVDRLFRV
                     AHTLKGNAGMVGFNEFVKLTHVLENVLDRLRSRTLGVNPEVIETLFHAFDALKATAEV
                     FANGEPFESSPAYERALSDLFALCDADEVSATEATERDLEVEVYFRPEFWSSGEDLTT
                     FLDELAMLGEVRHVETLFDRVPPLAELAPEQCRLGLRAHLRTTSGQMGVMGVCFLSVD
                     EENIKIRELGAKEQPEPEVPAAEQKSEVHHEAAPAPAREQTKTKAGSSARASTIRVDA
                     DKLDRLMDLVGELVIGISQVQDRNCSRVAVERLDALGRELQDQVMSLRMLPIHDTFER
                     FRRPMRDLAKELGRELDFVLSGGTTQVDKKVLESLVDPLKHMLRNCVSHGLETPADRV
                     AAGKPSSGTIHLSAAQREGHVLIEISDDGRGIDSARVLAKAEERGLVSRGQSLSQRQI
                     YDLLFQPGFSTASELSEISGRGVGLDVVQRAVQALRGNIEVESRLGHGTTFRIRLPLT
                     LAIVDGMNVLVGNETVTIPLLSVVELIASGPRTIKTIEGKNEYVDLRGELLPVLRLSD
                     ALALSESDSRPRDPHVVVVETERRKFGLLVDNVLGMAQTVIKPLDRAYDLFGHMKHDF
                     VKPRGVNGAAILGDGNVGIILDVHGLETTAFGAT"
BASE COUNT          519 a         1002 c         1022 g          583 t
ORIGIN      
        1 aactcgcggt cgttgaggcg gagggccgcg gtgagggagt cggctccccg agtctggatc
       61 ggcgccgggt tggtcatggt gtggtgtcga gcaacgagag cgctcccagc cgcgtcagca
      121 gtgatttcac gctgtcgctc gcgtcgcaga gccggtgtga gcgagaagtg gttacgaacg
      181 cggccaagac ctggactccc ttcacgtcga tgctttcgac gccagccaag tcgagctcgc
      241 gaggctcgcc cgcgggcgct gcgctctcgg cgagcaacaa aagctcccgg aacgatgcac
      301 aggcatccgc gcccagcgag ccgctggcgc gccagcgcga tccttctacc gagagtcgta
      361 tcatgttgct ccgaacgcgg tggtctcgag cccgtgcacg tccaagatga tcccgacgtt
      421 gccgtcaccg aggatcgcgg ccccattcac gccgcgcggc ttgacgaagt cgtgcttcat
      481 gtgaccgaac aggtcgtagg cgcggtctag cggcttgatg accgtctgcg ccataccgag
      541 cacgttgtcc accaagagcc cgaacttgcg gcgctcggtc tcgacgacga cgacgtgggg
      601 atcgcgtggt cgcgagtcgc tttcgctgag cgccagcgcg tcggagagcc gcagcacggg
      661 caggagctct ccacgcaagt cgacgtactc gttcttgcct tcgatggtct tgatggtgcg
      721 cggacccgac gcgatgagct ccacgacgct gagcagcgga atcgtgaccg tctcgttacc
      781 cacgaggacg ttcatgccgt cgacgattgc gagcgtgagc gggaggcgaa tccggaaagt
      841 cgtcccgtgg ccgagtcggg actcgacctc gatgttgccg cgaagggcct ggaccgcgcg
      901 ctgaacgacg tcgagcccga cgccccgacc ggagatttcg ctgagctccg aggcggtgga
      961 aaagcccggc tgaaacaaga ggtcgtagat ttgtcgttgg ctgaggctct ggccgcggct
     1021 cacgagtccg cgctcctcgg cttttgccaa tacgcgtgcg ctgtcgatgc cacgcccgtc
     1081 gtcgctgatt tcgatgagca cgtgaccttc gcgctgcgcg gccgacaggt gaatggtccc
     1141 agagctcggt ttccctgcgg cgactcggtc cgcgggggtc tccaggccgt gcgagacaca
     1201 gttgcgcagc atgtgcttga gcgggtcgac gagcgactcg aggaccttct tgtctacctg
     1261 cgtcgtcccg ccgctgagca cgaagtcgag ctcgcgccct agctccttgg cgaggtcacg
     1321 catcggacgg cggaagcgct cgaacgtgtc gtggatgggg agcatgcgca gcgacatcac
     1381 ctggtcctgc aactcgcgac cgagcgcatc caaacgctcg accgctaccc gtgagcagtt
     1441 gcggtcctgt acctgcgaaa tgccgatcac gagctccccg accagatcca tcaggcggtc
     1501 gagcttgtcc gcgtccacgc gaatcgtgct ggcgcgagcg ctcgagcccg ccttggtctt
     1561 cgtttgctcg cgagcggggg ctggcgcggc ctcgtgatgc acctcggact tttgctcggc
     1621 ggcgggcact tcgggctcgg gctgttcttt tgcgccgagc tcgcggatct tgatgttctc
     1681 ttcgtcgacc gatagaaagc aaacgcccat tacgcccatt tggcccgacg tcgtgcgcag
     1741 gtgcgcgcgg aggccgagcc ggcactgttc gggagcgagc tcggcgagcg gagggacgcg
     1801 atcgaacagg gtttcgacgt gccgcacctc gccgagcatc gcgagctcgt cgaggaacgt
     1861 cgtgaggtct tcgcccgagc tccagaactc ggggcggaag tagacttcga cctcgaggtc
     1921 gcgctcggtt gcctccgtcg cgctgacttc atccgcgtcg cacagggcga agagatcgct
     1981 cagcgctctc tcgtatgcag ggctgctctc gaaaggctcg ccgttggcga agacttcggc
     2041 ggttgccttg agcgcgtcga acgcgtgaaa aagtgtctcg atgacttcgg ggttcacccc
     2101 cagcgtgcgc gagcgcaggc gatccagcac gttttcgagc acgtgggtga gcttcacgaa
     2161 ctcgttgaac cccaccattc cggcgttgcc cttgagcgtg tgcgcgacgc ggaacaagcg
     2221 atcgacgcaa gccgccggat cgtcggtgtg ctcgagttcg agcagacctt cctcgagcgc
     2281 cgccaagagc tcttctgtct ctcggtggaa ggtcaccacc aactgatcga tcgtcatgaa
     2341 tggctcacta aacggtcgga aaagcgcccg cgataagcgc atccgcgcgc catgaaagag
     2401 tatttcagct cagctatcgt catgttccgc ctacgctcgc cacgctgaag caggaaacgt
     2461 acggcgggtt cgccgccagg gagcgccacg cgcgtgtagc gtgcaagcgg ctccgtacgc
     2521 acatgactgc tcgcgccgag tgcggagaat cgccggccgg ctctctccgc tccgcgaaat
     2581 gcgccggacg ggcgtcatca gggagttcgg gccgcgctcg cgcgcatact cggttccgct
     2641 ggttgaaata tggctccgca gtgtgttcac gacgcgaact cgtctctgtt cccctcgaac
     2701 tgtctcgatt ccgggcggag cacgtgccga ccgagagcga cgagttgctg cctctccaaa
     2761 gtccatttgc cgccgcagca aggttcgcga tagcgaacgc ccatttccga cgagctcttg
     2821 ctcgcgtcgg gatgcgctgg tgaagcggtc ggcatgtacg ctggctgtgc aagctcgagg
     2881 ccagcttcgg tttccgcgct cgcacgcagc ttttgcgtgc ttcgtgagtg aagcgcgcgg
     2941 acacgatccg attctttgga aaagcgttcc aggaatacgg acccacaatc gcaccctcgg
     3001 tgcgaccgcc gccctcatcg cgctctgagc atcgcgtgtg gtgcggctga caccgccccg
     3061 cgagacggcg aagctcttcg ttggcgacga gagcaccggc ttgtcgccgt tcgggtttcg
     3121 ccccca
//
LOCUS       DASTKS010001208         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1535691, whole genome shotgun sequence.
ACCESSION   DASTKS010001208 DASTKS010000000
VERSION     DASTKS010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535691"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFQ35_25745"
     CDS             complement(<1..183)
                     /locus_tag="VFQ35_25745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934142.1"
                     /translation="MKETIENAVGQGCEARSAEGWLEFLCRKKNGTGGRPVRATWPTE
                     NPSEDAGASQEVLADEH"
     gene            1363..2721
                     /locus_tag="VFQ35_25750"
     CDS             1363..2721
                     /locus_tag="VFQ35_25750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9934143.1"
                     /translation="MNEHRPAAAERPPAAFVPPPSKHSDEVVGASAFTALGLIPPLVR
                     AVLDEGYETPTPIQSGTIPCVLTGRDLLGCAQTGTGKTAAFVLPILQRLGATPTRGRL
                     RALVLTPTRELAAQIATRAAAYGRHVGLRHAVIYGGVSQRAQERELARNPELLVATPG
                     RLLDLFRQGFIDFGAIEVFVLDEADTMLDMGFIHDVRKIVAEIPKERQTLLFSATMPP
                     AIEQLARTILKDPERVSVTPQATTAETVDQAVYFVDKADKRVLLERVLATGVERALVF
                     TRTKHGADRVAKQLVRAGIEAAAIHGNKSQNARERALEAFRRGTSNVLVATDIAARGI
                     DIRGVSHVVNYELPDVAESYVHRIGRTGRAGASGRAVSFCAQEERPLLRDIERLIRKR
                     IPTLDLAAVLGAASARASNDAGDEGAESDSSARDDVGSAPRGAAPYEQRRPRFQARNR
                     FR"
     gene            2965..4128
                     /locus_tag="VFQ35_25755"
     CDS             2965..4128
                     /locus_tag="VFQ35_25755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9934144.1"
                     /translation="MPIFSERRDRFEVSDDKSRLDLDRIHRNLTESYWAKGIPRELVA
                     RSIERSMCFGLYDGQEQIGFARVISDETTFAYLCDVYVEPPYRGQALGSWLVRAVLSH
                     PELRGLRRFMLATRDAHRFYEPHGFKPPANPGHFLQIVKENAYGADMGAGNSVPVPPE
                     VRYLEATLSPPSIAYTRGSDLGAARAHPSDAPYEPSEEPSWPTGSLIQGPTSTRLIDA
                     TQRVVRVRPLGPSEGSLLRDLRVGALRESPHTFGEQLEDALNRPEHEWVEQARALTDP
                     TGPRVFIAEVDEWPAGLVLAVEDPFDATICRVGGMWVDPTKRRRRAGICMLEAVRSWA
                     REREKRRVRLWVHEDSPSARAVYEAFGFKYTGSRKRFPREPDRELLEMDLRVD"
     gene            complement(4133..4861)
                     /locus_tag="VFQ35_25760"
     CDS             complement(4133..4861)
                     /locus_tag="VFQ35_25760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET9934145.1"
                     /translation="MAAIAKADPAQHQGCELSSDVRAASGEKGRLVETWAAFTPGVAY
                     VAARPEDNAISLVVSVGDGFREVGLYWPTPHDPSYAYRRMSRLPYSSNLLGNDFDQTI
                     IVLREPIELVVDGEKKTYRPIDYAGNTLGIDGCGGNDQLHGGDGNDHLYDYAGENELR
                     GYGGRDWLEGVGGYFSGGAGDDCVTADGGSSFTRIFGDEGDDVLQSVGARGTAQGGDG
                     TDSCVATLYGTCENEEPAVCLGWN"
     gene            complement(4950..>5257)
                     /locus_tag="VFQ35_25765"
     CDS             complement(4950..>5257)
                     /locus_tag="VFQ35_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9934146.1"
                     /translation="LAKSRQSSKKLELRHCPRVHLKMIAVDGAYLYLGSANFTGAGLG
                     ARGDGRRNFEMGFGTDDDVLLDAAQQRFDRIWSGRDCGACRLRSECPAPLDVRRRSR"
BASE COUNT          915 a         1860 c         1652 g          830 t
ORIGIN      
        1 gtgctcgtcg gccaagactt cttgcgaagc accagcatcc tcgctggggt tttcggtcgg
       61 ccacgtcgcg cgcaccgggc ggcccccggt gccgttcttc ttgcgacaca gaaactcgag
      121 ccagccttct gcgctccggg cctcgcaacc ctgaccgacc gcgttctcga tggtctcctt
      181 catcgcattc gcgaacgacg cgggagcctc tgccgcaacg ctcggcgcgc tcgttggaac
      241 gacagccacg ccactcgaca ccgcgggtgg agaagcactc gtcgcgggaa gtacaaccgc
      301 ggcgccgctc gacggaaaag tcgagttcgc actgccaacg gcggcggcgt tgctagcgcc
      361 agcctccttt ttactgcacc cgacgagaac gatacccacc cccaacacga ccgagcgtat
      421 tctcaccacc agcgcactcc ttccactccg cgagccagta tagcggcccg cgcaccgttc
      481 atcgcgtctc gagcaaccgt gctttgccga gccccgcccg tcttttgacc gagggtcgaa
      541 acaaagctct cacgaccgca tcgcgcatcg acaacgtcga gcgttgcagc ggatatgcca
      601 ccctgcccca cgactctttg tcgcagattt tggggactcg gcgggcttcg cgccgcgtcg
      661 ccacggacat gtcggcgtgg tcaccgagac acggctggag ggtcgtgcac gcgtgcgaga
      721 gttgcgtagc gcgaaaatgc ctgctagtaa gcgcaccgtc agaaattcct tggctgcggc
      781 cggtgcaccc ctactgaaag ggctcggcac ctggaaggcc gccaagcgca tgactgatgg
      841 cgccgcttcg ggctttttct cagaggcagc gctcagccct ctctgccttc gcggtgtgcg
      901 cgcagaacgg gcccagcctc ctcgcgccga ccgcagacaa agtccgaatc agtgcactct
      961 catgaccgac gccccgagtc ggcgcgcaaa gcgtcccact cggagcgagg cccctctcac
     1021 ccgcgcgccg aacgccccgc ctcggcccct caccgcgaag actccgctcg gagctctgac
     1081 tccaaccgca acgcgagcgt tggaagcttc cgccaagacg accgcggctc cgcccacggc
     1141 gacccgcggc gcagcttccg ccccgacgcg ggagagcgcg cgcccaacga tcgtggcccc
     1201 cgacgaaaca gcgctcctcc tccagcgaga gcgccgcaag atcgctttgc gtcgccgccc
     1261 ccgaaacggg caccgcacgc cgagcgctcg ttcactcgcc cggaacccgc accgcacagt
     1321 caccgccgcc cggcgcgcga tccgcgccac gctcgtgggc ccgtgaacga gcatcggccg
     1381 gcagcagcgg agcggcctcc ggccgctttc gtcccaccgc cctcgaagca tagcgacgaa
     1441 gtcgtgggag catcggcgtt caccgcgctc gggttgattc cgccgctcgt gcgcgccgtg
     1501 ctcgacgaag gttacgagac tcctacgccg atccagagcg gcacgattcc gtgcgtgctc
     1561 accgggcgcg acttgctcgg ttgcgcacag accggcaccg gcaaaaccgc cgcgttcgtg
     1621 ctgcccattt tgcagcgcct gggcgcgacg ccgacccgcg gacgcttacg agcgctcgta
     1681 ctcacgccga ctcgcgagct cgctgcgcaa atcgcgacgc gcgcggctgc ctacggccgg
     1741 cacgtaggct tgaggcacgc ggtcatctac ggaggcgtga gccaacgggc ccaggagcgc
     1801 gaactcgcgc ggaacccgga gctcttggtg gcaaccccgg ggcgcctact cgacttgttc
     1861 aggcagggct tcatcgactt cggcgcgatc gaggtgttcg tgctcgacga ggccgacacg
     1921 atgctcgaca tgggcttcat ccacgacgtt cgcaagatcg tcgccgagat cccgaaagag
     1981 cgacagactc tgttgttctc tgccaccatg ccgcccgcga tcgaacagct cgcccgcacg
     2041 atcttgaaag atccggagcg cgtgtccgtg acgccgcaag ccacgacggc ggagacggtc
     2101 gaccaagcgg tatacttcgt cgacaaggcc gacaagcgcg tgctgctcga gcgcgtgctc
     2161 gcgaccggcg tcgaacgcgc gctggttttc actcgaacga agcacggcgc cgatcgcgtc
     2221 gccaagcagc tcgtgcgcgc cggcatcgaa gcggccgcga tccacggcaa caagtctcag
     2281 aacgcccgcg agcgagcgct cgaggctttc cggcgcggaa ccagcaacgt gctcgtcgcg
     2341 accgacatcg ccgcgcgcgg catcgacatc cgcggagttt cgcacgtcgt gaactacgag
     2401 cttcccgatg tcgccgaaag ctacgtccat cggatcggcc gcactgggcg cgccggcgcg
     2461 agcggtcgag ccgtctcgtt ttgcgcgcaa gaagagcggc cgctgctgcg agacatcgag
     2521 cggctcatcc gcaagcgcat cccgacgctc gacttggccg cggtgctcgg cgccgcgtct
     2581 gcccgcgcgt ccaacgacgc cggcgacgag ggagccgaaa gcgattcttc ggcgcgcgac
     2641 gacgttggct ccgcaccccg cggagcggca ccgtacgagc agcgacgccc ccgatttcag
     2701 gcgagaaatc gcttccgctg agctcgtagc agccgcgtcg agagaaagct gagcggccgg
     2761 ggtttctcgg ccgctgagag ctcccctcac ggggcggggc gctccctgcc aaagctctcg
     2821 aacgatcggg ctctggcaac gaacgtggct gccggccgag tttctcgggc aagggtgggg
     2881 gctgccccgg gcgggaccga gtagcaatcc ggccgcaacg gcggtattcg ctcggctgac
     2941 gtgtcgtcga gtgacaaccc agcgatgccg atcttctcgg agagacgaga ccgattcgag
     3001 gtgagcgacg acaagtcgcg cctcgacctg gaccgcatcc accggaacct caccgagtcg
     3061 tactgggcga aggggatccc acgagagctc gtcgcgcgca gcatcgagcg ctcgatgtgt
     3121 ttcggcctgt acgacggcca agagcaaatc ggttttgcgc gcgtcatcag cgatgagacg
     3181 acgttcgcgt acttgtgcga cgtgtacgtg gaacctccgt accgcggcca agcgctcggc
     3241 tcgtggctcg tccgagcggt gctgtcgcat cccgagctca gggggcttcg gcggttcatg
     3301 ctcgcgaccc gtgacgcgca tcgcttttac gaaccgcacg gcttcaaacc cccggcgaac
     3361 ccggggcact tcctacaaat cgtcaaggag aacgcctacg gcgcggatat gggcgccgga
     3421 aactccgtgc cggtgccacc cgaggtgcgg tacctcgaag caacgctctc tccgccgtcc
     3481 atcgcctaca cgcgaggctc cgatctgggc gcggcgcggg cacacccttc ggacgcaccc
     3541 tacgagccga gcgaggaacc gtcgtggccg accggctctc tcatccaagg ccccacgtcg
     3601 acgcgcctca tcgatgccac gcagcgggtc gtgcgcgtgc gcccgctcgg gcccagcgag
     3661 ggctccctgc tgcgcgattt gcgagtgggc gctttgcgcg aatccccgca cacattcggt
     3721 gagcagctcg aggacgcgct gaaccgccca gaacatgagt gggtcgaaca agcgcgcgcg
     3781 ctgaccgatc cgacgggacc ccgcgtattc atcgctgaag tcgacgaatg gccggcgggg
     3841 ctcgtgctcg cagtggaaga tccgttcgac gccacgattt gtcgcgtcgg cgggatgtgg
     3901 gtcgacccta ccaagcggcg acgtcgcgcc ggcatctgca tgctcgaagc ggtgcgctcc
     3961 tgggcgaggg agcgtgagaa gcggcgcgtg cgattgtggg tgcacgagga ttcgccgtcg
     4021 gcgcgcgccg tctacgaggc gttcggcttc aaatacacgg gctcgcgaaa gcgctttcca
     4081 cgcgaaccag accgcgagct gctcgagatg gatctccggg tcgactgagc cttcagttcc
     4141 agcccaggca cacggccggc tcttcgtttt cgcaagttcc gtacaaggtc gcgacgcagc
     4201 tgtcggtgcc gtcacctccc tgagccgtgc cgcgcgcgcc gacgctttgc aagacgtcgt
     4261 cgccctcgtc tccgaagatg cgagtgaaac tcgagccacc atccgccgtg acacagtcgt
     4321 ctccggcgcc gccgctgaag tagccgccta ccccctccaa ccaatcgcgc ccgccgtaac
     4381 cgcgcagctc attttcgccg gcgtagtcgt acaggtgatc gttgccgtca ccgccgtgca
     4441 actggtcgtt gcctccgcag ccgtcgatgc ccagcgtgtt tcccgcgtag tcgatggggc
     4501 gatacgtctt cttttcgcca tctacgacga gctcgatggg ctcgcgcagc acgatgatgg
     4561 tctgatcgaa atcgttgccg agcaggttgc tgctgtacgg caaccgcgac atgcgacgat
     4621 aagcgtatga ggggtcgtgc ggcgtcggcc aatagaggcc aacctcacga aacccatcgc
     4681 cgaccgaaac gacgagcgaa atcgcgttgt cttcaggacg ggcagcgacg tatgcgacgc
     4741 ccggcgtgaa cgcggcccac gtctcgacca agcgcccctt ctcgcccgac gcggcgcgaa
     4801 cgtcggacga aagctcgcag ccttggtgtt gcgctgggtc ggccttcgca atcgcagcca
     4861 cactcgaaac cgacccgcca atggccaggg ccgcgcccca ggcccaacaa cggcgtgaaa
     4921 cgacagggtt ccccatgcag cggccaaatt taccgcgaac gccgccgaac gtcgaggggg
     4981 gccgggcact cggagcgcaa ccgacacgca ccgcagtcgc gccccgacca aatgcgatcg
     5041 aagcgctgct gggcggcgtc gagcaagacg tcgtcgtccg tcccgaaccc catttcgaaa
     5101 ttgcggcgtc cgtcgccacg cgcaccgagt cccgctccag tgaaattcgc gctgcctagg
     5161 tagaggtagg cgccatcgac cgcgatcatt ttcaagtgga cgcgaggaca atgtcggagc
     5221 tcgagctttt tcgagctttg ccggctcttt gccagct
//
LOCUS       DASTKS010001209         7343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1311989, whole genome shotgun sequence.
ACCESSION   DASTKS010001209 DASTKS010000000
VERSION     DASTKS010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311989"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFQ35_25770"
     CDS             complement(<1..422)
                     /locus_tag="VFQ35_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter, permease protein"
                     /protein_id="HET9934147.1"
                     /translation="MASSVTKGATPRMSLLVLPFVGILAVIGIWAIVSSTLVPSLPSP
                     LKTWETSRPYIIEPFEKRGELDQGILRFTWYSLERVAKGYSIAIIMGAPLGFLLGMSP
                     FFRGSFDPIIQILRPVSPLAWLPLGLVIFQKPEPAGIFT"
     gene            complement(524..1819)
                     /locus_tag="VFQ35_25775"
     CDS             complement(524..1819)
                     /locus_tag="VFQ35_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9934148.1"
                     /translation="MSDSISRRHFVSGAAGLGAAALLPAACKKTEPSVSAAKTDAPVS
                     SSAASSSKPEVTDLRFGMIALTDCSPIVIAHEKGLFKKYGINSTVIKGASWAAIRDSL
                     SNGDIQATHMLLGMPLASTMGLLGAPVKPMVIPWLLNRNGQAITMKADLKGKISNDPK
                     ALKPLVEAAKKSGSPMTFAMTFPPGTHAMWIRYYLAAGGINPDKDIALTTIPPPQMVA
                     NMKVGKMDGFCVGEPWNARAIADGIGFTAITTQEMWKDHPEKVCAFSEEFAQKNPKTV
                     KAVLKALHEASVWLDDLKNRKEQAEIVSRPTYINCPPEGILPRLMGEYDYGDGRKKQD
                     ENYMIFSSRNCNFPEPKYAKWWLSQFRRWGMTSAAPDYEGIAKRVMRFDLYQEAMKEL
                     GVAVAEPSDAPETLFDGTVYDPKDPEKYATSFAVHSLKA"
     gene            complement(1841..2437)
                     /locus_tag="VFQ35_25780"
     CDS             complement(1841..2437)
                     /locus_tag="VFQ35_25780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934149.1"
                     /translation="MNPVLGALYGLKLSPELRLGLFLGVTLPIGGGGGDTPKPERKAA
                     NAAGIAARNGMDNAMFAVNDFTVFPGIGLAFISGGFTAQLEATVLQLTRVRGDKAQPD
                     SSRTNFTSGVHLGYFILPQLSIGAELRHQRWLSTPKAVEQNSAARDTTTFAVGPRLHL
                     KVGEKMWMRPGVSFSMPFDKPNSDSKYKNIQLDIPFVF"
     gene            2436..2783
                     /locus_tag="VFQ35_25785"
     CDS             2436..2783
                     /locus_tag="VFQ35_25785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934150.1"
                     /translation="MRLIADFAVPGATFCTTPSRTSGLNESLSLQLASKVLTATLPDF
                     PAAFSYKPKVESERTAFTGTTPRSWKGSEYGGGLGFASAGAGGGSVSGGVGAATAGAF
                     SGAGAATGADAGP"
     gene            complement(2978..3934)
                     /locus_tag="VFQ35_25790"
     CDS             complement(2978..3934)
                     /locus_tag="VFQ35_25790"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET9934151.1"
                     /translation="MSERTPPLVALLESRMSPELARLVEKHGGAPLSVPALRESPAFS
                     VEHAHSVIDELASGRHEFVVFMTGVAVSLLFEMAEQAGRRPELVRSLRILNTVCRGPK
                     PTAALRGFGVPPTLSAREPFTSAEVIDAMSNVELRGRHVLLLHYGERSETLAETLLAQ
                     RAELAELWLYRWLMPLDVAPLEELVISLARSEVDALVVTCQAQFRHLYEVAVRIGLEK
                     QLVKALNRDVTVAAVGPTCAAILEAHRVSVDVIPDHPKMGPLVLALMRHLEHSRASAK
                     RASNSSLVAFAKKTTPELARGGLQTGTYLATELPRTRKSSPV"
     gene            4825..5295
                     /gene="bfr"
                     /locus_tag="VFQ35_25795"
     CDS             4825..5295
                     /gene="bfr"
                     /locus_tag="VFQ35_25795"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET9934152.1"
                     /translation="MRSDPEIIQALNEILTSELTAINQYFIHFRMLENWGYKRLAKKK
                     REESIEEMKHADRVIERILYLEGIPNMQRLNPVKVGEEPIEMHKLDLELEVEAVDRLN
                     RAITLCAQKNDAGTRELVESILEEEEDAIDWLEAQLHLVQDIGRERYLAQQIND"
     gene            complement(5429..5776)
                     /locus_tag="VFQ35_25800"
     CDS             complement(5429..5776)
                     /locus_tag="VFQ35_25800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934153.1"
                     /translation="MKQVSESKSKRRVGKALRATGSTLMGLTLSLLALSACSGAGEGE
                     SELGVEVAHTEQAIDPCAQCYVRYNGCVKLANERFGICLKTCTSQSVCMSQHASELRA
                     CQAQLDACTYNHC"
     gene            complement(6024..7280)
                     /locus_tag="VFQ35_25805"
     CDS             complement(6024..7280)
                     /locus_tag="VFQ35_25805"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9934154.1"
                     /translation="MTDDSSFARLMESAGAGATARATRRLRAGEAIEANVIQISQDSV
                     FVDVGTPSDGRIARAEFEDKDGTLRVKVGDRVRATVVDPRPDGPVLAVSFGHGSSIDT
                     GALELLRTSGAPVEGEVTKVIKGGLEVTLGSTRAFCPASQFDLSHVPDFNPYVGQKFD
                     FKVIDVRDNGRSVVVSRRSLLEDRRREAMAGAKEKLVIGSDVECTVTQLGRHGAVVDI
                     EGLEGFIHLSELAPHRVERAEDVLRVGERVTARILLVEDSPKGLKVRLSLRALQATPA
                     GSVQSGAAPADEVLKGTVTRTGQHGVFVQTSLGEGMIPLRELGLPPGADHRRAWPPGK
                     EVNVVVVNRAGGKLTFSATQVARVEERKNYREFTGAAPAQSASGLGSLGDLLRDKLKG
                     SAASAAPTKAERAAKGFSDGSVRRGR"
BASE COUNT         1336 a         2350 c         2321 g         1336 t
ORIGIN      
        1 gtgaagatgc cggcgggctc gggcttctga aagatcacga ggccgagtgg caaccaagcg
       61 aggggcgaca ccgggcgcaa gatctgaatg atcggatcga agctgccgcg aaagaacggg
      121 gacatgccca ggaggaaccc gagcggcgca cccatgatga tcgcgatcga ataacctttc
      181 gcgacgcgct cgagcgaata ccacgtgaag cgcaggattc cttggtcgag ctcaccgcgt
      241 ttctcgaatg gctcgatgat gtacggcctg ctcgtctccc aggttttgag aggcgaaggc
      301 aggctgggca cgagggtcga gctcacgatc gcccaaatgc cgatgacggc gagaatgccg
      361 acgaagggca aaactaggag cgacatacgg ggcgtcgccc ctttggtgac cgaactagcc
      421 atgacgtcct ctttcgcggt tcgaccgctc tcccattctc atcttcttac gttccggttt
      481 cgcgctcgtg tctgtttcga ttccctgtcg tgttgtgcgc ggatcaagcc ttcaggctgt
      541 ggacggcgaa cgaagtcgcg tacttctctg ggtctttcgg gtcgtagacg gttccatcga
      601 agagcgtctc gggcgcgtcg ctcggctctg ccacggcgac gccgagctct ttcatggcct
      661 cttggtagag gtcgaagcgc atcacccgct tcgcgatgcc ctcatagtcg ggcgcggcgc
      721 tcgtcatgcc ccagcgtcgg aactgcgaga gccaccattt cgcgtacttc ggctccggaa
      781 agttgcagtt tcgactactg aagatcatgt agttttcgtc ctgtttcttg cgtccgtcgc
      841 cgtagtcgta ttcacccatc aagcgcggca agatgccctc cggcgggcag ttgatgtacg
      901 tcgggcgcga cacgatctcg gcttgctcct tgcggttttt gagatcgtcg agccacacgc
      961 tcgcctcgtg cagcgccttc aacaccgcct tcaccgtctt cgggttcttc tgcgcgaact
     1021 cctcgctgaa cgcgcagacc ttttcggggt ggtctttcca catctcttgg gtcgtgatgg
     1081 cggtaaagcc gatgccgtcg gcgatggcgc gcgcattcca gggctcgccc acgcaaaagc
     1141 cgtccatctt gcccaccttc atgttcgcca ccatttgtgg cggcgggatc gtcgtgagtg
     1201 cgatgtcctt gtccggattg atgccgccgg cagccaggta atagcgtatc cacatggcgt
     1261 gcgtgccagg gggaaaggtc atcgcgaacg tcatgggcga cccgctcttc ttcgccgctt
     1321 cgaccaaggg cttcagcgct ttggggtcgt tcgagatctt ccccttcaaa tccgccttca
     1381 tcgtgatcgc ctgcccgttg cgattgagga gccacgggat gaccatcggc ttcaccggag
     1441 cgccgaggag gcccatggtc gaggcgagcg gcattccgag cagcatgtgc gttgcctgga
     1501 tgtccccgtt cgaaagcgag tcgcgaatcg cggcccagct cgcgcccttg atgaccgtcg
     1561 agttgatgcc gtacttcttg aagagcccct tttcgtgagc aatcacgatg ggtgagcagt
     1621 cggtcagcgc gatcataccg aagcgcaaat cggtcacctc gggcttgctg ctactcgcgg
     1681 cgctcgagct caccggggcg tcggtcttgg cggcgctgac cgagggctcc gtctttttgc
     1741 acgctgccgg cagcagcgcg gcggctccga gcccggcggc cccgctcacg aaatgacgac
     1801 gactaatcga atcactcatg gtgttttcct cgagttcggc tcaaaagacg aagggaatat
     1861 cgagttggat gttcttgtat ttgctgtcgc tgttcggctt gtcgaaaggc atcgagaagg
     1921 acacgccggg gcgcatccac atcttctccc ccaccttcaa atgaaggcgc gggccgaccg
     1981 cgaaggtcgt cgtgtcacgc gccgctgaat tctgctcgac cgctttcggc gtcgagagcc
     2041 aacgctgatg gcgcagctcc gcaccaatcg acagctgggg aaggatgaag taaccgaggt
     2101 gtacgcccga ggtgaagttg gtccgcgacg agtcgggctg ggccttgtca cctcgcacgc
     2161 gagtcaactg cagcacggtc gcctcgagct gagccgtgaa tccaccgctg atgaacgcga
     2221 gaccaatgcc agggaagaca gtaaagtcgt tcaccgcgaa cattgcgttg tccattccgt
     2281 ttcgggcagc gatcccggct gcgttcgcag cttttctttc gggtttgggt gtatcgcctc
     2341 caccgccgcc aattggcagc gtgaccccga ggaacaggcc caaacgcagc tcgggagaca
     2401 acttcagccc gtaaagagcg ccaaggaccg ggttcatgag gctcatcgcc gatttcgcgg
     2461 tgcctggggc cacgttctgc acgaccccga gccggacgag cgggctgaac gagtcgctga
     2521 gcttgcaact cgcgagcaaa gtgctcaccg cgacacttcc ggacttccct gcggcgttct
     2581 cgtacaagcc caaagtggag tccgagcgca ccgcgttcac tggaacgaca ccacgcagct
     2641 ggaaggggag tgaatacggc ggaggcttgg gcttcgcgtc ggctggcgcc ggcggtggct
     2701 ccgtcagcgg cggcgtggga gccgcgaccg cgggggcctt ctctggcgca ggcgcggcga
     2761 cgggcgccga cgctggccct tgagaggctt gcggggcttg aaccggcgcc ggagtcagcg
     2821 ggggcgtcgc gcttccggca ggcggcaagg ccgcgtcgcc gctctgcgca cgtgcggtcg
     2881 aagccgcgaa ggcgtgtagc aagcaatagg taagaccaac gcgtgcaatt cgatgggcaa
     2941 tcttcatcgt tctctgtctt tgggcgagta aggcggccta taccggcgac gacttgcgtg
     3001 ttctcggcag ctcggtcgcg aggtatgttc ctgtttgcaa tccaccccga gctagctcgg
     3061 gcgtagtctt ttttgcgaat gcgacgagac tcgagttgga cgctcgcttc gcgctcgcgc
     3121 gggaatgttc gaggtggcgc atcaatgcga gcaccaacgg ccccatcttc gggtgatcgg
     3181 ggatgacgtc gacgctcacg cggtgcgcct cgaggatggc ggcacaagtt ggtccgaccg
     3241 cagcgacggt gacgtcgcga ttcagcgcct tgaccagctg cttttcgagg ccaattcgga
     3301 ccgcgacctc gtacagatga cgaaactgcg cctggcacgt cacgacgagc gcgtcgacct
     3361 cggagcgcgc gagcgaaatc accaactctt cgagaggcgc cacgtcgagc ggcatgagcc
     3421 accggtagag ccagagttca gcgagctcgg ctcgttgtgc gagcaacgtc tcggccaacg
     3481 tctcgctgcg ttcgccgtaa tgaagcaaca gaacgtggcg accgcgcagc tcgacgttcg
     3541 acatcgcgtc gatgacttcg gccgacgtga agggctcccg cgccgagagc gtcggcggga
     3601 cgccgaagcc gcgcagcgcc gccgtcggct tcgggccgcg acacaccgtg ttcaaaatac
     3661 gcagagaacg aacgagctcc gggcgtcgcc ccgcttgctc cgccatctcg aagagcagcg
     3721 acaccgcgac accggtcatg aaaaccacga actcgtggcg accgctcgcg agctcgtcga
     3781 tgacggagtg cgcgtgctcg acgctgaacg ccgggctctc gcgcagcgcc gggacgctca
     3841 gcggcgcgcc gccgtgcttt tctacgaggc gggcgagctc tggactcatg cgcgattcga
     3901 gcagagcgac gagcggcgga gtgcgttcgg acatgcggtg ttcgagcgca gctggtggcg
     3961 gcgctcgtca gatgcaatcg caacgtgcgt gccacagtcg cgcactcaca cgccgaagcg
     4021 gcgaaacacg ccgcgccaga ggctgcgaaa cgcgggcgat acgcgcagta ccccgaagtt
     4081 tgcgacagtc ctgtcagcga aagagatttg aagcgacgag gttgtcgccc gcgccccgcg
     4141 actcgaagct tcagccgcgt ttcggcgcgc agatcctgca gcccgcccgc gggaagctct
     4201 ctctctcgtc ctcggtaatc aaccgtgttc gcggtgcgtg acacgcgaac gaaacgacat
     4261 cttcgtcacg tatttccggc acgcgcgcta cgtgtttctc tcgcgttgcg cgcgactttc
     4321 gtcaagcggc agagcgcttg gtcggagctt ccgcgtggtg cgacggcgcc cgccgctcga
     4381 gccggagcaa gcgctcgatc atcggttcac agcccccgca gcaagtgccc gcgccgcacg
     4441 cttcgccgac ggcctcgacg gtatcggctc ctgcgtggat ggcagctcgg atctcacggt
     4501 cgcacagggc attgcagtga cagatgagca acggacttac ctctcgtcga aacctcctgc
     4561 aggctagcct gttgaaagtg attgtcaaaa tcacattcgc gcctggcgtg acctgacggc
     4621 gcacccggcg gccataacac caagtaacta ttggttattt tgtaaccacc aaccaccgat
     4681 cggtcggcgg gttccgagcc gcgatctgat tgaaatcatg caactggaag tgcgcgacgg
     4741 ccgatgtgcg cgaggacggc cgtgacgctt gcagagagca aggctttccc ggcatggtcc
     4801 gattcctttg gaggccatcg cccaatgcgt tctgatcctg aaatcatcca agcgctgaac
     4861 gaaatcctga cgagcgagct gacggcgata aaccagtact tcattcactt tcgcatgctc
     4921 gaaaactggg gttacaagcg cctcgccaag aagaagcgcg aagaatccat cgaggagatg
     4981 aagcacgccg atcgcgtcat cgagcgcatc ctctatctcg agggcatccc caacatgcag
     5041 cgactgaacc cggtcaaggt cggggaagag ccgatcgaga tgcacaagct cgatttggag
     5101 ctcgaggtcg aggccgtcga ccgcttgaac cgcgccatca cgctctgtgc acagaaaaac
     5161 gacgccggca cgcgcgagct cgtcgagagc atcttggaag aggaagagga cgcgatcgat
     5221 tggctcgagg cgcagctgca cctcgttcaa gacatcggtc gagagcgtta cctcgcccag
     5281 caaatcaacg actgacgacg aggagggcga gacgagtctc gacgactcta ctcgcgccgg
     5341 cgagtgctcc tcgccacgat cgaatcgtcc ggcgcggttc gcggccgcgg gggcaactcg
     5401 atcgtggcga aggaagcagc ggcggagctc agcagtggtt gtaggtgcaa gcgtcgagct
     5461 gcgcctgaca agctctgagc tcactcgcgt gttggctcat gcagacgctc tgagaagtgc
     5521 acgtcttgag acagataccg aagcgctcgt tggcgagctt cacgcagccg ttgtaacgga
     5581 cgtaacactg cgcacacgga tcgattgctt gctccgtgtg cgcgacctcg accccgagct
     5641 cggactcacc ctccccggcg ccgctgcacg ccgagagcgc gagcagcgaa agcgtcaagc
     5701 ccatcagcgt cgagccggtg gcgcggagcg cctttccgac gcgacgcttc gacttcgatt
     5761 ccgaaacctg cttcatccgt cgacctcctt gtaagcgagc ctccgcggcg ggcgattgct
     5821 caccttgagt cgcccacgcg cgagaacctc gacgcgcgac gaagcaggct ccgtgccgcc
     5881 ttatgtattc gcgggcttgc gcattaccgt ggcgttcgcg gctcgcaacc agcacttgcg
     5941 gacgcaaccc gaggttgccc aggctcctgg atcagcagcg acttcgcagg ttctgccgcc
     6001 gccgtatgcg cgagcgacga agctcagcgg ccgcgacgca cgctgccgtc actgaaaccc
     6061 ttcgcagcgc gctctgcttt cgttggagca gcggaggcgg cgctgccctt gagcttgtct
     6121 cgcagcaaat cgccgaggct gccgaggccg gacgccgact gagccggcgc ggctccggtg
     6181 aactcccggt agttcttacg ttcttcgacg cgtgccacct gcgttgcgct gaacgtgagc
     6241 ttgccgcctg cccggttcac gacaacgacg ttcacctctt tgccgggcgg ccacgcgcga
     6301 cggtgatcgg cgccgggggg caagccgagc tcacgcagcg ggatcatccc ctcacccagc
     6361 gaagtctgga cgaacacgcc gtgttgcccc gtgcgcgtca ccgttccctt gagcacttcg
     6421 tccgcgggag ccgcaccgct ttgaaccgac cccgccggcg tggcctgcag cgcacgcagc
     6481 gagagccgca ccttcaggcc tttcggcgag tcctcgacca agaggatacg agccgtcacg
     6541 cgctcaccga cgcgcagcac gtcttcggcg cgctccacgc ggtgcggcgc tagctccgag
     6601 aggtggatga acccttcgag cccttcgatg tcgacgaccg cgccatggcg accgagctgg
     6661 gtcacggtgc actcgacgtc ggagccgatg acgagcttct ccttcgcgcc agccatcgcc
     6721 tcacgacggc gatcctcgag tagagagcgg cgcgacacga ccacgctgcg gccgttgtcg
     6781 cgcacatcga tcaccttgaa gtcgaacttc tgccccacgt aggggttgaa gtcgggcacg
     6841 tgggagagat cgaactgcga cgcggggcag aatgcgcgag tcgaaccgag cgtgacctcg
     6901 agcccgcctt tgatcacctt cgtgacctcg ccctcgaccg gagcgccgct cgtgcgaagc
     6961 agctcgagcg cgcccgtgtc gatcgatgag ccgtgaccga acgacacggc gagcacggga
     7021 ccatccggac gagggtcgac caccgtcgcg cgcacgcggt cccccacttt cacgcgcaac
     7081 gtgccgtcct tgtcttcgaa ttcggcgcgc gcgatgcgac cgtcgctcgg cgtaccgacg
     7141 tcgacgaaga cgctatcttg ggagatctgg atcacgttgg cttcgatggc ctcgccagcg
     7201 cgcagacgcc gcgtcgcccg ggccgttgcg ccagcacccg cggactccat caaacgcgca
     7261 aaactcgaat cgtcggtcat ggggcgcgga gcataagcca gttttcgaac tgtgccagac
     7321 ggggtcagaa agtgccctcc gtg
//
LOCUS       DASTKS010001210         4240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_640295, whole genome shotgun sequence.
ACCESSION   DASTKS010001210 DASTKS010000000
VERSION     DASTKS010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640295"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(581..2044)
                     /locus_tag="VFQ35_25810"
     CDS             complement(581..2044)
                     /locus_tag="VFQ35_25810"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9934155.1"
                     /translation="MTELTLTVPGPEPLPILGGAGRLLSFVLDPVRYAGELFERYGRI
                     AVLVRGRPTRVVSTEPGVPGTVFLYGPELNRQLLTGHEEFNKCALTGPLYPSEPPNER
                     RQPLLRTMTGLFHVNQQEHRQHRRMLMPAFHKTRIETYRDAMVEIVESVLATFRAGET
                     RDICPDMMELTLRVATKTLFGSDLGEQGVAVGRDLHRWLRLFRLGAALPWDVPLTPYR
                     RWLDLTRTIDEKMVRILADRRASGVSGDDMLSMLIQTRDEDGGRLSEDELIGHAGVIF
                     AAGHETSSNALAWTLLLLSQHPNIARDLYDELHGVLHGDAPRVDQLPSLPLLDRVVKE
                     SLRVLPPVPFNHRIVGTETELGGYVLPRNTEILSSIYHTQRMPEHFAEPSRFNPDRWL
                     KEDPGPYLYNPFSAGPRMCIGATFAMMEIKIVLAMFLQRYRFELAPNSRVDRYISITM
                     APRPGVRMRLHPQDGAFEKSSRSLRGNIHEMVSFSSN"
     gene            complement(2044..3009)
                     /locus_tag="VFQ35_25815"
     CDS             complement(2044..3009)
                     /locus_tag="VFQ35_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934156.1"
                     /translation="MSLTAEPLERTFRTTPNRGFGLGFAPPKLGINAGDLVTARNTHE
                     EDCSRRSRSGFVALAVSFVAFGCASGQGDCHAETWSGTCQLVSVTKTRQSELPLPNLT
                     LQAVYRPQPGGNGPTLMPPDQPKDFTVLDRYEDALRAHIDSHKAARCYVNPPPPGQCV
                     PGQLVIDVPEFDAAHADVTPENQGPKGCAQIEATSSQDRVTQAKTSQTIINERFQFGE
                     QSSDLPPDAASALDALAARLKQSPNLQCVGVVGAWIRGESVAVAFARARAVRDQLVSR
                     GVEPERLIALTVDPPTLSASTAAEPPNPNDRKVTLSVLLDLPPAR"
     gene            3052..3855
                     /locus_tag="VFQ35_25820"
     CDS             3052..3855
                     /locus_tag="VFQ35_25820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934157.1"
                     /translation="MAGPFAYRFGVFLAIALGAMGLSSASGLAHAAPEPPAAPSAPPS
                     LELPDGVVAGESTVVPVPNDRPAFVIHAAADNRRAIVYLPGLCGNVRAVEAWKQAAAR
                     SGTLVGLLGDKPCGGGRYHWGKSLDVIEARIERALVAVKAARGGMLDIEHPILFGYSQ
                     GADRAERLAQRYPRKYLRVVLGGSPNEPGLKRLGDTQAVAVFGGELETFGRMQAGAEV
                     LAAAGKTARFFLFPRAKHGDFGPEGNRKMGELFDWLLAEDEDLKSAQRE"
     gene            complement(3843..>4240)
                     /locus_tag="VFQ35_25825"
     CDS             complement(3843..>4240)
                     /locus_tag="VFQ35_25825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934158.1"
                     /translation="LGTVTGYVPLLRPHLPATLHTPWRTDVTPEQVIEVFGKAESARL
                     DRGWLTLRGLSAGSAPVAEFDCGVPVHIGRDGFSFSVELTGRCSVRPQDSRPVAISLS
                     GPLRVEATGIPGIQVSGRLEAQVTHSYSR"
BASE COUNT          744 a         1228 c         1535 g          733 t
ORIGIN      
        1 ccgaaagtga aggtcttcaa gtccaccgtc agccgtgagg gacgaggcga caggcgagcg
       61 ctgggcgcca atgccacagt cctctacgcc caaaagcgaa tatgtataca tacaatcgag
      121 agcaagacca aggatccgca aatcaggcaa ttttagccga aatatgcggg gtgtcgcgcg
      181 ctgacgcgtg atgtcaggat cgtctgtccc tgcgatggcg ttatcggcgc tcgaacgccc
      241 gctcagaccc cctcgatttc tcggcatttc acgccgtcgg ggccggcgat ggtgagtatg
      301 tatacatagt tgtcgctgga gatgaaggac gaggccgctc gcgacaattg gcgccaggtt
      361 agaggtcagg aggcggccaa acctgctgcg cgcgacaaat tggggagccg cgcgagaaca
      421 actgaccagg gcggagcgag ggcgagcgcg gagcgagggc gagcgcggag cgagggccag
      481 ggcggagcga gggccagggc ggagcgaggg cggagcgagg gcggagcgag ggcgagcgcg
      541 gagcgggggc gagcgcggag cgggggcgag cgcggagcgg tcagttggaa gagaaggaaa
      601 ccatttcgtg gatgttgccg cgaagggagc gggagctctt ttcgaaggcg ccgtcttgag
      661 ggtggaggcg catgcgcacg ccgggtcggg gcgccatggt gatgctgatg tagcggtcca
      721 ctcgactgtt cggagcgagc tcgaagcgat agcgctggag gaacatggcg agcacgattt
      781 tgatttccat catggcgaag gtcgcgccga tgcacatgcg cgggcctgcg ctgaagggat
      841 tgtagaggta aggtccggga tcttctttga gccagcgatc ggggttgaag cgggaaggtt
      901 ccgcgaagtg ctcgggcatc cgctgggtgt gatagatgct gctcaagatt tcggtgttgc
      961 gcggtaagac gtagccacca agctcggttt cggtgccgac gattcggtga ttgaagggga
     1021 cgggcggcaa gacgcgaagg gattccttta cgacgcgatc gagcaggggg agggagggga
     1081 gctggtcgac tcgaggggcg tcgccgtgca agacgccgtg gagttcgtcg taaaggtcgc
     1141 gcgcgatgtt gggatgctgt gagagcagga ggagcgtcca cgcgagcgcg ttcgagctgg
     1201 tttcgtgccc cgcggcgaaa atgacgccgg cgtgtccgat gagctcgtct tcgctgaggc
     1261 gtccgccgtc ttcgtcgcgc gtttggatca acatggagag catgtcgtcg ccggacacgc
     1321 cggatgcgcg tcgatcggcc aagattcgca ccatcttttc gtcgatagtg cgggttagat
     1381 cgagccagcg gcgatacggc gtgagtggta cgtcccacgg gagggcggcg cccaaacgaa
     1441 agagcctgag ccagcgatgc aagtcgcgcc cgacggctac gccctgctcg ccgagatcgg
     1501 agccgaacag ggtcttcgtc gcgacgcgaa gcgtgagctc catcatgtcg ggacagatgt
     1561 cgcgggtctc gccggcgcga aaggttgcga gcaccgactc gacgatttcg accattgcgt
     1621 cgcggtaggt ttcgatgcgc gttttgtgaa acgcgggcat cagcatgcgt cggtgttggc
     1681 ggtgctcctg ctgattgacg tgaaagagcc ccgtcatcgt acgaaggagc ggctggcgtc
     1741 gttcgttggg aggctcgctc ggatagaggg ggcctgtgag cgcgcacttg ttgaactctt
     1801 cgtgaccggt caaaagctgg cggttcagct cgggcccgta caaaaaaacg gtgccgggaa
     1861 cgccaggctc cgtcgaaact acgcgggtcg gacgcccgcg cacgagcacg gcgatgcgcc
     1921 catagcgctc gaaaagctca ccggcataac gcacggggtc gagcacgaaa ctgaggagcc
     1981 tgcccgcacc gcccaagatc gggagcggtt cgggtccggg gacggtcagc gtgagctcgg
     2041 tcatcagcgc gccgggggaa gatcgaggag cacgctgagc gtcacttttc gatcgttggg
     2101 gttcgggggt tcggcggcgg tcgaagcgct caacgtcgga gggtcgaccg tgagcgctat
     2161 caaacgttcg ggctcgacgc cgcgcgacac gagctgatct cgcacggcgc gcgccctcgc
     2221 gaacgcgacc gctacgctct cgccgcggat ccaagcgccg acgacgccga cgcattgcag
     2281 gttcgggctc tgcttgaggc gtgcggcgag cgcgtcgagc gcggacgcgg cgtcgggagg
     2341 caagtcggac gactgctcgc cgaactgaaa acgttcgttg atgatggttt gcgaggtctt
     2401 ggcttgcgtg acgcggtctt gcgagctcgt ggcttctatc tgcgcgcagc ccttgggtcc
     2461 ctgattttct ggggttacgt ccgcgtgggc ggcgtcgaac tcggggacgt cgatcaccaa
     2521 ttggccgggt acgcattgcc cggggggagg tgggttgacg taacagcgcg ccgccttgtg
     2581 gctgtcgatg tgcgcacgca acgcgtcctc gtaccgatcc agaaccgtga aatctttcgg
     2641 ctgatctgga ggcatcaacg tcgggccgtt gccgccgggc tgaggccggt aaaccgcctg
     2701 aagggtgaga ttcggcaacg ggagctcgct ttgccgcgtc ttcgtcacgg aaacgagctg
     2761 gcaggtgcca gaccaagtct cggcgtggca gtcgccttgg ccgctggcgc agccgaacgc
     2821 cacgaacgag accgcgagcg ctacgaaccc gctccgcgat cgcctcgaac agtcctcttc
     2881 atgtgtgttg cgcgccgtca cgagatcccc tgcgttgatg ccaagctttg gcggggcaaa
     2941 tcctagcccg aagccgcggt tcggagtcgt tcgaaaagtg cgctcgagcg gctccgccgt
     3001 aagcgacaaa cttgcagcgc gcgctgacgt ccatcatcgt agatggaagg cgtggctggt
     3061 ccttttgcgt accgcttcgg agttttcctc gcgatcgcgt tgggtgcgat gggcctgtcg
     3121 agcgcctccg gcttggcgca cgcggcgccg gagcccccag ctgcaccctc agcgccgccc
     3181 tcactggaat tgccggacgg ggtcgtcgca ggcgagagca ccgtcgtgcc cgtcccgaac
     3241 gatagaccgg ccttcgtgat tcacgccgcc gccgacaatc ggcgcgccat cgtttacctc
     3301 ccggggctct gcggcaacgt gcgcgcggtc gaagcttgga agcaagctgc ggcgcgctcg
     3361 gggacgctcg ttggcctttt gggggacaag ccttgcggcg gtggacgtta ccactggggc
     3421 aagagtctcg acgtcatcga ggcacgcatc gagcgcgcgc tcgtcgcggt caaagccgca
     3481 cgcggcggca tgctcgacat cgagcatcca atcttgttcg gctactcgca aggcgccgat
     3541 cgcgccgagc ggctcgcaca gcgttacccg cggaagtatc tgcgcgtggt gctcggtggc
     3601 tcgccgaacg agccgggact gaagcggctc ggtgacacgc aagcggtcgc ggtgttcgga
     3661 ggcgagctcg agacgttcgg gcgaatgcag gccggggccg aggttttggc cgctgcgggc
     3721 aagaccgcgc gcttctttct ctttccgcgc gccaagcacg gcgacttcgg gcccgaaggc
     3781 aaccgcaaga tgggcgagct cttcgattgg ctgctcgcgg aggatgagga cctgaagagc
     3841 gctcagcgcg agtaggagtg cgtcacttgc gcttcgagtc gccccgagac ttgaatgccc
     3901 ggaatgcccg ttgcttcgac gcgcagcggt ccgctcaggg agattgctac ggggcgtgag
     3961 tcttgggggc gcacgctgca gcgccccgtg agctcgacgc tgaagctgaa cccgtcgcga
     4021 ccgatgtgca ccggcacgcc gcaatcgaac tccgcgacgg gagcgctgcc cgccgagagc
     4081 ccgcgcaacg tgagccatcc gcgatcgagg cgcgccgact ccgctttccc gaagacttcg
     4141 atcacttgct cgggcgtcac gtcggtgcgc catggcgtgt gcagcgtcgc gggtaggtgg
     4201 ggtcgcagca gaggtacgta gccagtcacg gttccgagat
//
LOCUS       DASTKS010001211         8039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1536388, whole genome shotgun sequence.
ACCESSION   DASTKS010001211 DASTKS010000000
VERSION     DASTKS010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536388"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFQ35_25830"
     CDS             complement(<1..631)
                     /locus_tag="VFQ35_25830"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET9934159.1"
                     /translation="MPVGPLERRITPWLLALVALILVGGVAYTNARGYVATQAAVREA
                     LSAREAANGVLSQLKDLETGTRGFMLTGEERFLDPYISARARLPQSLHALAELGKADG
                     ELGSSARRIGELANQKLDYAERLVRFGRNDKVQLKETLALLEAGKTVMDALRTEVERM
                     IERTDRRLEQRERETANASFRLQLALAGMLCGAVVIALGGLSGARRQIEA"
     gene            complement(731..1627)
                     /locus_tag="VFQ35_25835"
     CDS             complement(731..1627)
                     /locus_tag="VFQ35_25835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934160.1"
                     /translation="MPGYDEALASLYRAPLAEFVSERKRLAKELKAGGDADGARRLSE
                     RARPNISAWAVNQLYWQARDEFETMLETAAPLRAGGVVGGAEHRDSLNVLRRRATSIL
                     EQSGHAASDATMRRIMTTLSAIAATGGFAPDAPGTLSADRDPPGFEAVGIADDEPRPV
                     MESPKSATVRESPSHRQRGLKPGKSQALEPSRQGAVEEGQSEARERQRREDAVRRERE
                     RQEQERAEQEREARERERKRREGERRKVRAELETAQRDVEAKELEVDEQRNALTRAEQ
                     GRDRARARAEELERKLAELGDE"
     gene            complement(1929..4121)
                     /locus_tag="VFQ35_25840"
     CDS             complement(1929..4121)
                     /locus_tag="VFQ35_25840"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9934161.1"
                     /translation="MSTPLEAENDQDNRYHVAASSAMTPAETFVLKVDDTFCVLDRMG
                     DVCPEGRSEQGLYHGGTKFVSLLRLRLRGQRPLLLSSGMLENNLLQRVDLTNPDLFDE
                     EHKIVIPYGALHVSRSKFCQNAACFERIQIANYGGERVHLEFTLDFAANFVDVFEVRG
                     TQREARGKLLAPLVERGATSLRYLGRDEVVRTTRIAFSPPPNELLPGRARFEIDLEGK
                     ETFTLNIHIACQIGGANELPERSARSFDAEWGNVLSRVKEREANECQIITSNVEFGDW
                     LERSRSDLRMLLTDTAQGPYPYAGVPWFSTPFGRDGIWTALEVLWARPDVARGVLLFL
                     SEHQAKTEDPSADAEPGKILHEMRWGEMATLREIPFGCYYGSIDSTPLYLMLAHRYYR
                     RTGDRALLQSIWPNLLAAVEWIDKYGDSDGDGFVEYGRRSKDGLIQQGWKDSNDSVFH
                     ADGKLAQGPIALAEVQAYTFGALQGMSELAAAFGDHERSLNLAQRASALRERFDAVFF
                     SEELGMYALALDGEKKPCLVKSSNAGHALYTGIARRERADAVGRALFSDAMFSGWGVR
                     TLAVTEQRYNPMSYHNGSIWPHDNAIIAAGLADYGMKQHAVRVTEAMFDAARFMNLTR
                     LPELFCGFPRELEQGPTLYPVACSPQAWASGAVYMLLSALLGLELDALNERVVLRHAV
                     LPPCLEHLEIRGLQVGRATLDLRLDRHPHDVGVTVTGKQGNVEVIAVK"
     gene            complement(4118..5239)
                     /locus_tag="VFQ35_25845"
     CDS             complement(4118..5239)
                     /locus_tag="VFQ35_25845"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9934162.1"
                     /translation="MSSKSDISGAHERVRTKGSTLRIAQVAPLYESVPPQAYGGTERV
                     VHFLTEELVAMGHDVTLFASGDSQTSARLVPCCERSLRLDPNCKDPLAYHMIMLDQLV
                     SRRDEFDIIHFHIDYLHYPVSRLLGWPHVTTLHGRLDLPELVPMYEQYSTMPLVSISN
                     SQRKPVEGANFVATVLHGLPKDLLRFSPKGGDYFAFLGRISPEKRVDRAIEIAIAMNT
                     PIKVAAKIDKMDKEYHESGIVHLLEHPLVEFVGEIGDDQKSEFLGNARALLFPIDWPE
                     PFGLVMIEAMACGTPVVAFRCGAVPEVLEDGVSGFIVDDMDAAMAAARKVGDLSRERV
                     RQAFDQRFTARRMAEEYVDVYRTLSAGRPERHGTLRESA"
     gene            5612..5917
                     /gene="trxA"
                     /locus_tag="VFQ35_25850"
     CDS             5612..5917
                     /gene="trxA"
                     /locus_tag="VFQ35_25850"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9934163.1"
                     /translation="MDESEFDEIGARARVPILVDFWATWCGPCRMVAPELKRAAHELA
                     GKALVLKVDTDRHPGLASRFNVSGIPNFVVLKGGRVLRQQAGALSHKNLVALVQSSA"
     gene            complement(6123..7307)
                     /locus_tag="VFQ35_25855"
     CDS             complement(6123..7307)
                     /locus_tag="VFQ35_25855"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9934164.1"
                     /translation="MRWSYSLLLCLVCACRLVPKKTGPVGEPPAPLPTPPDVVPVHEV
                     GEVAAPPVETASRPLSGFLRGINLGNALDAPREGAWGVTLKPEHFRMAKAKGLDHVRL
                     PVRFSAHADEKPPYEIEGAILDRVEWAIEQARENGLSIIIDLHHYQELMKNPEAHADR
                     LVALWRQVAEHFKDAPPTVAFELINEPCDKLTPEYLNPLTARALAAVRASNPTRIVIA
                     DSYFWASADQLKNLSLPEDPNVVASFHMYQPILFTHQGMPWMGPEYQTRGVVFPGPGQ
                     RPIVPAPATEKVEWVKTWFTGYNSVPIAENPNGPKAIFDYLKTVEAYVAASGRRVYMG
                     EFGVADSADPRSRENWLRLVRVEVEKRHIGWALWDDGARFRAMNVGWDSWIAPIEAGL
                     FH"
     gene            complement(7361..>8039)
                     /locus_tag="VFQ35_25860"
     CDS             complement(7361..>8039)
                     /locus_tag="VFQ35_25860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934165.1"
                     /translation="PRCARRYRGRDVVDWLQDMGHYDVPITQQSNPEELRERANHYVS
                     GRGGGRDLDLRAFAVRGMKLYGPLLSVSGDKLRFAPELKRHLDAADDVYRSINRAIDA
                     YIDQHGIVAPAASTYVPPWEPKEEIEELELSKSGITSAIFCTGFNTDFSFIREDVFDS
                     KGAPLHRRGVTSVDGLYFIGLPWLHTWGSGRFSAVGRDAMYIVEHLLAYTSETLSGHA
                     VSARAAG"
BASE COUNT         1379 a         2632 c         2525 g         1503 t
ORIGIN      
        1 ccgcctcgat ttgccgccga gcgcccgaca gacctccgag cgcgatcacg acggcgccgc
       61 acagcatgcc cgcgagagcg agctggagtc gaaagctcgc gttcgcggtt tctcgctcgc
      121 gctgctcgag gcgccggtca gtacgctcga tcatgcgctc tacctcggtg cgcagagcgt
      181 ccatcacggt cttgcccgct tcgagcaatg cgagagtctc tttcagttga accttgtcgt
      241 tgcgaccgaa ccggaccaag cgctctgcgt agtcgagctt ctggtttgcg agctcgccga
      301 tgcggcgcgc ggagctgccg agctcaccgt ctgccttccc gagctccgcg agcgcgtgca
      361 agctttgcgg aagccgtgcc cgagcgctga tgtagggatc caggaagcgc tcttcaccgg
      421 tgagcatgaa gccgcgcgtc ccggtctcca ggtccttcag ctgcgagaga acaccgttcg
      481 cagcctcgcg agcggaaagc gcttcgcgca cggccgcctg ggtagccacg taccctcgag
      541 cattcgtgta agcgacgccc ccaaccaaaa tgagcgcaac gagggcgagt agccacggtg
      601 tgattcgacg ctcgaggggc ccgaccggca cggataaagc tccgcccgaa ttagcggcca
      661 gctaccccgt ctctaaaggc ccggtgccta ccaattatgt tcaatcaaat ccgggcgttc
      721 gctgcagctg ctactcgtca cccagctcgg cgagcttccg ctcgagctct tcggcccgag
      781 cgcgcgcccg atcgcgcccc tgttcggcgc gtgtaagtgc attccgctgc tcgtccacct
      841 cgagctcctt cgcctccacg tctcgctgcg cggtctcgag ctcggcgcga accttgcgcc
      901 gctcgccctc acggcgcttt cgctcgcgct ctcgcgcttc tctctcctgc tcggctcgtt
      961 cttgttcttg ccgctcacgt tcgcgccgaa cagcgtcttc gcgtcgctgc cgctcacgag
     1021 cttcggattg tccctcttcg acggcccctt gccgactcgg ctcgagagcc tgcgatttgc
     1081 ccggcttcag gcctcgttgg cgatgcgagg gagactcccg gaccgtagcg ctcttcggcg
     1141 attccatgac gggccgcggt tcgtcgtcgg caatgcccac cgcttcgaag ccgggagggt
     1201 ctcgatccgc gctgagcgtc cccggcgcat cgggtgcgaa gccccccgtc gccgcgatcg
     1261 ccgaaagcgt cgtcatgatt ctccgcatcg tggcgtcgct ggcggcgtga cccgactgct
     1321 ccagtatgct cgtcgcacgg cgcctcagaa cattgagcga atcacggtgc tcggcccctc
     1381 ccactacgcc accggcgcgt agtggggcag ccgtctccag catcgtctcg aactcatcgc
     1441 gagcctgcca atagagttga ttgacggccc acgctgaaat gttcggacgc gctcgctcgc
     1501 tcaagcgccg tgctccgtcg gcgtcgccac cggctttgag ctccttggcg agccgcttcc
     1561 gctccgagac gaattcggcg agcggcgctc gatagaggga agcaagggct tcgtcgtagc
     1621 ctggcatggc gcgcgaccag attctctccg tcggccgcca cccgcaagtc tcgcgacggg
     1681 gcgaggtcct aatcaacccc gcgggttggt ctcgcgtttg catccgtgtc gcggcatgcc
     1741 ttggctcctg cggatcgttc ggctcggccc tttcgccatt tttcgcgacg ctaacgggcg
     1801 attcttctgg acctggcggc ggcgtcggct ccgaccattg cccgaattgc tgccgctccg
     1861 ctacttgatg cgctgatgga attcgccacg gcggggcact tttgtcgagg ggagcgtggg
     1921 cccctgctct acttcacggc gatgacctcg acgttcccct gcttgccggt gacggtgacg
     1981 cccacgtcgt gaggatgtcg gtcgaggcgc aagtcgagcg tcgcgcgtcc cacctgcagc
     2041 ccacggattt cgaggtgctc gaggcaaggg ggaagtacag cgtgtcgtag caccacgcgc
     2101 tcgttcagcg cgtcgagctc gagcccgagc aacgcggaaa ggagcatgta gaccgcgccc
     2161 gaggcccatg cctgcgggga acacgcgacc gggtagagcg tcggtccctg ttcgagctcg
     2221 cgcgggaacc cgcagaagag ctccgggagg cgcgtgaggt tcatgaaccg agccgcatcg
     2281 aacatggctt ccgtgacccg taccgcgtgc tgcttcatgc cgtagtcggc gaggcccgcg
     2341 gcgatgatcg cgttgtcgtg cggccaaatc gagccgttgt gatacgacat cgggttatag
     2401 cgctgctcgg tgaccgcgag cgtgcgcacg ccccaaccgg agaacatggc atccgagaac
     2461 aatgcgcgcc ccaccgcgtc ggctcgctcg cgacgcgcga tgcccgtgta cagcgcgtgc
     2521 cccgcgttgg agctcttgac gagacaaggc ttcttctctc cgtcgagagc cagcgcatac
     2581 atgccgagct cttccgaaaa gaacaccgca tcgaagcgct cgcgcaacgc gcttgcccgc
     2641 tgcgccaggt tcaacgagcg ctcgtgatcg ccgaacgcgg ccgcgagctc ggacatgcct
     2701 tgaagcgctc cgaacgtata cgcttgcacc tccgcgagcg cgatcggtcc ctgcgcgagc
     2761 ttgccatccg cgtggaacac cgagtcgtta gagtctttcc atccttgttg gatgaggcca
     2821 tccttggagc gcctaccgta ctcgacgaag ccatcgccgt cgctgtcgcc gtacttgtcg
     2881 atccactcga ccgccgcgag caggttgggc caaatcgact gaagcagcgc gcgatcgccc
     2941 gtacgccggt agtagcgatg cgcgagcatg aggtagaggg gcgtcgaatc gatgctgccg
     3001 tagtagcaac cgaatgggat ctcgcgcagc gtcgccatct caccccagcg catctcgtgc
     3061 aagatcttgc cgggctcggc gtcggcgctc gggtcttcgg tcttggcttg gtgctccgag
     3121 agaaacagca gcacgccgcg cgcgacgtcg ggacgtgccc atagcacctc gagcgcggtc
     3181 caaatcccgt cgcggccgaa cggcgtgctg aaccacggaa cacccgcgta agggtacggc
     3241 ccttgcgccg tatcggtgag cagcatgcgc agatccgaac gggaacgctc gagccaatca
     3301 ccgaactcca cgttcgaagt gatgatctgg cattcgtttg cctcacgctc tttcacgcgc
     3361 gagagcacgt ttccccactc ggcgtcgaaa gatcgcgcgc tccgctcggg gagctcgttg
     3421 gctccaccga tctggcaagc gatgtgaatg ttcagggtga aggtttcttt gccctccaag
     3481 tcgatctcga agcgggcgcg gcccggcaag agctcgttcg gcggcgggga aaaggcgatg
     3541 cgagtcgtac gcaccacttc gtcccgcccc aagtatcgaa ggctcgtggc gcctcgctcg
     3601 acgagcggtg cgagcagctt gccgcgcgcc tcgcgttggg taccgcgcac ctcgaaaacg
     3661 tccacgaagt tcgccgcaaa gtcgagtgtg aattcgaggt gaacgcgctc ccctccgtag
     3721 tttgcgattt gaatacgttc gaagcacgcc gcgttttgac agaacttcga gcgcgagacg
     3781 tggagcgcgc cgtacggaat gacgatcttg tgctcttcgt cgaacaggtc cggattggtg
     3841 agatcgaccc gttgaagaag gttgttttcg agcatcccgg agctcagcag caacgggcgt
     3901 tggccgcgca gacggaggcg cagcaacgag acgaacttcg tgccgccgtg gtagagccct
     3961 tgctcactgc ggccctcggg gcaaacgtcg cccatccggt cgagcacgca gaaggtgtcg
     4021 tccactttga ggacgaaggt ctccgccggc gtcatcgcgc tcgatgccgc gacgtgatac
     4081 cggttgtcct gatcgttctc ggcttcgagg ggcgtgctca tgcagattct cgcagggtgc
     4141 cgtgccgctc agggcggcca gcggagagcg tccgatagac gtcgacgtat tcttcggcca
     4201 tgcgccgcgc cgtgaagcgt tggtcgaatg cctgacgcac tcgctcacgg ctgaggtcac
     4261 cgactttacg cgccgccgcc atcgccgcat ccatgtcgtc cacgatgaag ccactgacgc
     4321 cgtcttcgag cacctcgggc acggcgccac accggaatgc caccacgggc gttccgcagg
     4381 ccatcgcctc gatcatgacg agaccgaatg gttcgggcca atcgatggga aagagcagcg
     4441 cgcgggcgtt gcccaaaaat tcggacttct ggtcgtcgcc gatctcaccc acgaattcga
     4501 cgagcggatg ctcgagcagg tggacgatgc cgctctcgtg atactccttg tccatcttgt
     4561 cgatcttcgc cgcgaccttg atcggcgtat tcatggcgat ggcgatctcg atcgcgcgat
     4621 cgacgcgctt ttctggcgag atgcggccca agaaggcgaa gtagtcgccg cctttgggag
     4681 agaaccgaag gaggtccttc gggagcccgt gaagcaccgt cgccacgaag ttcgctccct
     4741 cgaccggctt tcgctgcgag ttcgagatcg agacgagcgg catcgtcgag tactgctcgt
     4801 acatgggcac gagctcgggc aggtccaagc gtccgtgcaa cgtcgtcacg tgcggccaac
     4861 cgagtagacg cgacaccggg tagtggaggt agtcgatgtg aaagtgaatg atgtcgaact
     4921 cgtctcgcct cgacacgagc tggtcgagca tgatcatgtg gtaggcgagc ggatctttgc
     4981 agttcgggtc gaggcgaagc gagcgttcgc agcacggcac gagtcgcgcg ctcgtctggg
     5041 agtctcccga ggcgaaaaga gtcacgtcat ggcccatcgc gacgagctct tccgtcagga
     5101 agtgcacgac ccgctccgta ccgccatatg cctgcggggg cacgctctcg taaagcggcg
     5161 ctacctgagc gatacgcaag gtggatccct tcgtgcgcac ccgctcgtgc gcgccgctga
     5221 tatctgattt cgaagacaca ccagacgctc agcaagttgg gtgccaccgg aacggacatt
     5281 cattgtcgaa gcgccccgct gggttcttct tagcgaacgt cagcatatcg gtcgtcatgc
     5341 ttcgggaatg gcgcaaccac gagcggcctt gcggcaaggc agagtgcccc aatgtggcgc
     5401 agcgggagcg ctccgcgccg ccccgaagcg tgcgccggag cccagcgaac agccggcttg
     5461 actcgacggt ttccgtgcgc aggttggttc aatgattcgc gcctgcccga gctgcggtca
     5521 aaaaaatagg attggccctg agcacttggc cgaccgcggg cgctgcggcg catgcaagca
     5581 gccaatcggt cccgtagatg agcccatcga ggtggacgag tccgagttcg acgagattgg
     5641 cgctcgggcg cgagttccca tccttgtcga cttttgggcg acgtggtgcg gcccctgtcg
     5701 catggtcgcc cccgagctaa aacgggccgc gcacgagcta gccgggaagg cgctggtgct
     5761 caaggtagac accgaccgcc atcccgggct cgcatcacgc ttcaacgtga gcggcatccc
     5821 gaacttcgtt gtgctcaagg gcggccgagt cttgcggcag caggctggcg cgctcagcca
     5881 caagaacctc gtcgctctgg tccagagctc ggcttgagct cactagctag aacgccgatc
     5941 agtctgactg ctcggcgttc tagccttggt ttgtgggggc ggcacgcgcc gtccctctct
     6001 tccgaaatga attcggaaga gacttcccca cgcggtcact ccgtgcggaa ccgtttcccg
     6061 ctaccttccg cgaattccta accgcttgtc gctcaaggaa ctgggcgcag caacgcgccg
     6121 tttcagtgaa atagcccagc ttcgatcggc gcgatccagc tgtcccagcc gacgttcatg
     6181 gctcggaacc gcgcgccgtc gtcccacaga gcccagccga tatggcgctt ttcgacctcg
     6241 acccgcacga ggcgcagcca gttttctctc gagcgcgggt cggcgctgtc ggccacgccg
     6301 aattcgccca tgtagacgcg gcgtccgctc gccgcgacgt aagcctcgac cgtcttcagg
     6361 taatcgaaga tagccttcgg tccgttcggg ttttcagcga tagggaccga gttgtatccc
     6421 gtaaaccagg tcttcaccca ctcgaccttc tccgtcgcgg gagcgggaac gatgggtcgc
     6481 tgtcccggcc cgggaaacac gactccccgg gtttggtact cgggtcccat ccacggcatg
     6541 ccctgatgag tgaacaagat gggctggtac atgtggaagg acgcgacgac gttcggatcc
     6601 tccggcagcg acaggttctt gagttggtcc gcgctcgccc agaaatagct gtcggcgatc
     6661 acgatgcgcg ttgggttgct ggcacgaacc gcggcgagag cgcgagcggt gagcgggttg
     6721 aggtactccg gcgtcagttt gtcgcaaggt tcgttgatga gctcgaacgc gacagtcggt
     6781 ggggcatcct tgaagtgttc ggccacctga cgccaaagcg ccacgagccg gtctgcgtgc
     6841 gcttccgggt tcttcatcaa ctcttggtag tggtgaaggt cgatgatgat cgaaaggccg
     6901 ttctcacgcg cttgctcgat ggcccactcc acgcgatcca gtatggcgcc ctcgatctcg
     6961 taggggggct tttcgtctgc atgcgcactg aaacgcaccg gtagcctcac gtgatcgagg
     7021 cctttggcct tcgccatgcg aaagtgttcc ggcttcaggg tcacgcccca ggcgccctcg
     7081 cgcggtgcat cgagcgcgtt accgaggttg atgccgcgca ggaagccgct caaaggccga
     7141 ctcgccgttt cgaccggcgg cgccgcgact tctcccacct cgtgcaccgg cacgacgtcg
     7201 gggggagtcg gcagcggcgc cggaggctct ccgactggcc cagtcttctt tggcaccagg
     7261 cgacacgcgc acacgagaca gagcaagagc gaatacgacc aacgcatgga ggccgcgact
     7321 atgccaccca ccgccaagcg agacgagcga gatagtgccg tcagccggca gcgcgagcgg
     7381 aaacggcgtg gccgctgagc gtttctgatg tgtaggccag caaatgctcg acgatgtaca
     7441 tcgcgtcgcg accgacggcc gagaagcgcc cggagcccca cgtgtgcagc cacggcagtc
     7501 cgatgaaata cagaccatct acggacgtca cgccgcgccg gtgcaagggc gcccccttcg
     7561 aatcgaagac gtcttctcgg atgaagctga agtcggtgtt gaacccagta cagaagatgg
     7621 cgctcgtgat cccgctcttc gagagctcga gctcttcgat ctcctctttc ggttcccacg
     7681 gtggcacgta ggtggacgcc gccggcgcca cgatgccgtg ctgatcgatg taggcgtcaa
     7741 tcgcccgatt gatacttcga tagacgtcat ccgcggcgtc caagtggcgc ttcagctccg
     7801 gggcgaagcg cagcttgtcc cccgacacgc tcaggagcgg gccgtagagt ttcatgcccc
     7861 tcacggcgaa cgcgcgtagg tccaaatctc ggccccctcc ccgccccgat acatagtggt
     7921 tcgctctttc gcgcagctct tctgggttgc tctgttgggt gatcggaacg tcgtaatgac
     7981 ccatgtcttg gagccaatcg acgacgtccc gaccccggta gcgccgggca catcgcggc
//
LOCUS       DASTKS010001212         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1312337, whole genome shotgun sequence.
ACCESSION   DASTKS010001212 DASTKS010000000
VERSION     DASTKS010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312337"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1886
                     /locus_tag="VFQ35_25865"
     CDS             <1..1886
                     /locus_tag="VFQ35_25865"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9934166.1"
                     /translation="GAKVYIQIEEGPPVLLGRVDVHGIEKLPERAQRRIQRRMESELG
                     MGKPFEELTFANAEAELKRRLEDHGYAYVELKRAANVDLPRHIASAGFWVELGPKSYF
                     GPVRIEGLGPLPEAPVRRALDLKEGDVYSRSELDEAKRALLDLSVFSGVSIEPDVPKK
                     ERAGTPPPETPEQPPKPGETRDDKSAQMAAEAGVPDQNALAHPERVPLVVKLERSKLR
                     SVHLGFGVRLDTQRADLHLIAGWEDSNFLGGFRRFLVEATPGVVLFPTRLPDFAKPKR
                     LLPQARLRSEFRQPGFLEARTNALVRAEISAYPLILSSKYDPSAPVLGYEDLRASAGL
                     ERSYWKLYGTATHNVQVSSPFAYSGELDPNLHTVVISYPELFASLDLRNDRVSPHQGA
                     YVSMDAQVAGAGGDARDVKLQPELRGYIPIARRFTLALRGTVGFLFPQNYGDTVARNA
                     SGDLTGINTPDLVRDIQIMFLRGFFSGGSGSNRGYAARDIGPHGTVPFYNPGQAMTGT
                     GSCRAVADATNGSQCSLPLGGFTLWESTLELRYPLLGPLTGTVFGDASDVSPYKVDFR
                     FDRPHLSVGLGLRYETPIGPVRFDFGYRVPGMQAADKSGEVDPATIWGLPVAASFGIG
                     QVF"
     gene            1886..>6505
                     /locus_tag="VFQ35_25870"
     CDS             1886..>6505
                     /locus_tag="VFQ35_25870"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9934167.1"
                     /translation="MANLRRKVSRVLAKVARWTGYLLAGVVGLVVVLLLCINLKPLRE
                     FIRARANAALADTFVGTLTIRRIGYISPYSLGGVDADVRDGKGRLVLRGLGLSATCHW
                     PGIVWDLIRKRPLTVTLSPVNSDHIEVVLIDDGKGSPTLASAFSPRTPSPEQPATQSA
                     PTTISIPDLRARHIWVHGGLQALPSIDTELSRLALSLRSKGSELDAKLSNLSVVARNL
                     PGQVNPRGKFLAQVNVRGEPQSKIKGFVDFNGTLAGAPTVLHGEFDDKRVGARLDARE
                     IPASTITAQVPALTPRGPTGLEVNARGTLPRIEFDGKLDNEALRAVLTGHVSLEETTF
                     AHAEVDAKQIDASGWVATAPKTNLALVAKVDAEVPKAGPTFVHYDVSVPSGSVQGAIT
                     PELKLRGKLVQSKDAGLSVDGALDADERGITLAARFLYTQGLKQNQAVTGSLSLELKD
                     SPRVRQLTGATLNGTVRANTELDLSNERILSAKANVALSPVVHGTDRIDSLSADVAAS
                     GSLKAPELDVRAQARNGNLGGQRFQRLNLSGSGTPARFVARGTVDRDERQRIEFGTTI
                     SSGNATELLGTTLAVLSRDEKPINLRARRVRIGGGQLRFEQVELDGPGTVALSGQYGG
                     GRADAEFDVQALDVGRLTRTLRVNTPIQRALVTAKGRVKGPLRRLQGELEAHVTQLDM
                     EHVRGGNVDVDLRIHNQVASGTVAANLGDSRVTAKVEQFVVPAPDAAITPTSLSGKLE
                     VRGSVELARVANALRSAGAPLERASGHLDIDLDAERAPGGPLMPRAELRVKTKSLKLV
                     EQRPPKKGVSDASDARATQPRSLDGVDVDATVSLDPQENKAKVLGSLFDQRGPMLELD
                     AETHLPKQGLPLGAALSATTVKVSARIPARELDQLPRSVRPPSMHGVVQANVEAEGNL
                     RSPKLMATAGVRGFRTREGGRRAMNAELMLRYDKARGELRGSASTSRQPNALFVDTRW
                     DGDLVGKFMDENAPLDLDAEVKLDRFPTGVVPMLADRSVKGPVSCDIKLEDWGKDAKL
                     TGTLDGSGMKLSGVQLSKLDVSFNTENERISALVDVREQDGTLRLDASTPLTWGHSIA
                     PTVDPHLKANLKARRFQLDTMSPFVSQYLSTLEGELNADFGLEMGQDAPKLQGSAEIR
                     DGVVQIPQVGQRFSDVQAKLSVDPNGKVRLEKLQARGTSGRVTADAEATLRGTNVESA
                     KLAARITKAEKLPVTLEGVALGDAWGKLNVNYRHADGTNDIRVDIPEFQMQMPEAGFG
                     NVQGLDPDEHIRIGVYRGDKTFAAIPTQPLRASESDTPPAEPPTVTRVAVHLGDSVWI
                     QRGTQAKVQLGGELNITSGEESQIRGRIDLRGGQLDVNGKTFNIENGTVTFEGDDPGN
                     PTIVATARWDAPDSYSVYAEYRGTVKAGKLTLRSEPPLTQPEIVSLLLFGSPEGSLGQ
                     SNGGGGGAAATAVGVAGDTAVRGFNRVMSDFTHLDVSARIDTSTGSARPEIVVQVTPR
                     LTTRVTRALGEPPPGESPDRTFLTLELRLQRSWAVSAVVGDRGASV"
BASE COUNT         1181 a         2126 c         2134 g         1064 t
ORIGIN      
        1 gtggcgccaa agtgtacatt cagatcgagg aaggcccccc ggttctcctc ggtcgcgtgg
       61 acgttcacgg catcgagaaa ctccccgagc gggcccagcg gcgcatccaa cgacgcatgg
      121 aaagtgagct cggcatgggc aagccgttcg aggagctcac ctttgcaaac gccgaggccg
      181 agctaaagcg ccggctcgaa gatcatggct acgcttacgt ggagctcaag cgcgccgcga
      241 acgtggatct gccccgccac atcgcgagcg ccgggttttg ggtcgagctc ggaccgaagt
      301 cgtacttcgg tcccgtgcgc atcgaagggc tcggcccttt gccggaagcg ccggtgcggc
      361 gtgccctcga cttgaaagag ggcgatgtct attcgcgctc ggagctcgac gaagcgaagc
      421 gagcgctgct cgacctgagc gtgttcagtg gcgtttcgat cgagccggac gttccgaaga
      481 aggagcgggc gggcacgccg ccgcccgaga ccccggagca accgccgaag ccgggtgaaa
      541 cccgagacga caagtccgcg cagatggccg cggaagcagg cgtgcccgac cagaatgcgc
      601 tggcacaccc agagcgtgtc cccctcgtcg tgaagctcga gcgctccaag ctccgcagtg
      661 tgcatctggg cttcggcgta cggctcgata cgcagcgcgc cgaccttcat ctgattgcgg
      721 ggtgggaaga cagcaacttc ctcgggggct ttcggcgatt cttggtcgag gcgacgccgg
      781 gtgtcgtgct ttttccaacg cgcttgcccg acttcgcgaa gccgaagcgc ctcttgccgc
      841 aggcacgttt gcgcagtgaa ttccggcagc caggttttct cgaagcgcgc acgaacgctc
      901 tcgtgcgcgc ggagatcagc gcttacccat tgatcttgag cagcaagtac gacccgagtg
      961 cgccagtcct cggctatgaa gacttgcgag cgtctgcggg cctcgaacgc tcttattgga
     1021 agctctatgg caccgcgaca cacaacgttc aggtgagcag ccccttcgcg tacagcggcg
     1081 agctcgatcc gaaccttcac acggtcgtca tttcttatcc agagctgttt gcgagcctcg
     1141 acttgcgcaa cgaccgcgtc tcgccccacc agggcgcgta cgtctcgatg gacgcccagg
     1201 tggcgggcgc gggcggcgac gcgcgcgacg tgaagctgca gccggagcta cgcggctaca
     1261 tcccgattgc gcggcgcttc actctcgctt tgcgcggcac ggtcggcttt ctgtttcccc
     1321 aaaactatgg cgatacggtc gcgcgcaacg cgagcgggga tctgaccggc atcaacacgc
     1381 cggatctcgt ccgcgatatc caaatcatgt tcttgcgagg gtttttctcc ggcggttccg
     1441 gctcgaatcg gggttacgcc gcgcgtgaca tcggtccgca cggtaccgtc cccttctaca
     1501 acccgggtca ggcgatgacc ggaacgggga gctgtcgagc cgtcgcggac gcgacgaacg
     1561 gatcgcagtg cagcttgccg ctcggcggct tcacgctgtg ggaaagtacg ctcgagctcc
     1621 gctacccact gctcgggccg ctcaccggca ccgtattcgg tgacgcgagc gacgtttctc
     1681 cttacaaggt cgactttcgc ttcgatcggc cgcatctcag cgttggtttg gggttgcgct
     1741 acgagacacc gattggaccg gtgcgcttcg atttcggata ccgcgtgccg gggatgcagg
     1801 cggccgacaa gagtggtgag gtggatcccg caacgatttg gggactcccc gtcgcggcga
     1861 gcttcgggat tggacaggtg ttctgatggc aaatcttcgc cgcaaggtca gccgggttct
     1921 cgccaaagtc gcgcgttgga ctggctacct tctggctggg gtcgtcgggc tcgtcgtcgt
     1981 gctgctcctc tgcatcaacc tgaagccgct gcgggagttc atccgcgcgc gggcgaacgc
     2041 ggcgctcgcc gataccttcg tcggcacgct gacgattcgt cgaatcggct acattagccc
     2101 ttattcactg ggcggcgtcg acgccgacgt tcgcgacggc aagggccggc tcgtcctacg
     2161 gggcctcggc ctgtcggcga cgtgccactg gcccggcatc gtttgggact tgatccgcaa
     2221 acgtccgctc acggtgacgc tgtcgccagt gaactcggat cacatcgaag tcgtcctgat
     2281 cgacgacggc aagggcagcc ccacgctcgc gagcgcgttt tcgccgcgca ctccttcacc
     2341 cgaacaacct gcaactcaat ctgcgccgac caccatttcg atccccgacc ttcgcgcgcg
     2401 gcacatttgg gtgcacggcg gtcttcaagc tcttcccagt atcgacacgg agctttcacg
     2461 gcttgcgctc agcctgcgga gcaaagggtc cgagctcgat gcaaagctct ccaatctgag
     2521 cgtcgttgcc cgcaacctcc ccgggcaggt gaacccgcgc ggcaagttct tggctcaggt
     2581 caacgttcgg ggcgagccgc aatccaagat caaaggcttc gtcgatttca acggcaccct
     2641 tgctggcgct cccacggtgc tccacggcga gttcgacgac aagcgcgtgg gagcgcgtct
     2701 cgacgcgcga gaaatccccg cgagtacgat cacggcgcaa gtcccggcgc tcactccgcg
     2761 cgggccaacg gggctcgagg tgaatgcccg cgggactttg ccgcgcatag agttcgacgg
     2821 aaagctcgac aacgaagcct tgcgcgcagt cctcaccggt cacgtgagcc tggaagagac
     2881 gacctttgcg cacgccgagg tcgatgcgaa gcaaatcgat gcgagcggct gggttgctac
     2941 ggcgccgaaa acgaatctcg cgctcgtcgc taaagtcgac gcggaagttc ccaaagccgg
     3001 acctacgttc gttcactatg acgtgagcgt gccgagcggc tccgtgcaag gcgcgatcac
     3061 tcccgagctc aagctcaggg gtaagctcgt ccagagcaaa gacgcgggct tgagcgtcga
     3121 tggagctctc gacgcggacg aacgcgggat caccttggcg gctcgatttc tctacacaca
     3181 gggcttgaag cagaaccagg cggtgacggg cagcctatca ctcgagctca aagactctcc
     3241 acgcgttcgt cagctcaccg gcgccacgct gaacggcact gtgcgggcca acaccgagct
     3301 cgatctctcg aacgagcgca tcttgtcggc caaggcgaac gtcgcgcttt cgcccgtggt
     3361 ccacggaacg gaccgcatcg actccctctc ggcggacgtc gccgcgagcg gttcgttgaa
     3421 agcgcccgag ctcgacgtgc gcgctcaggc acggaacggc aacctcggcg ggcagagatt
     3481 tcagcggttg aacctgtcgg gcagtggtac gcccgctcgc ttcgtcgctc gaggcacggt
     3541 cgaccgagac gagcgacagc gcatcgaatt cggcaccacc atctcgtcgg gcaatgcgac
     3601 cgagctcttg gggaccactt tggcggtatt gtctcgtgac gaaaagccga tcaatctccg
     3661 ggctcgtcgg gtgcgaattg gaggtggaca gctccgcttc gagcaggtcg agctcgacgg
     3721 accgggcacg gtcgcgttgt ctggacagta cggcggcggg cgcgccgacg cggagttcga
     3781 cgtgcaggct ctcgacgtgg gccggctgac gcgaaccctg cgcgtgaaca ctcccattca
     3841 gcgcgcgctc gtgaccgcga agggacgagt gaagggcccc ctccggcgcc tgcaaggcga
     3901 gttggaggcg catgtgactc agctcgatat ggaacacgtg cgaggcggca acgtggacgt
     3961 cgatctgcga atccacaatc aggtggcgtc cggaaccgta gcggcgaact tgggcgattc
     4021 gcgcgtcacc gcgaaggtcg agcagttcgt ggtgcccgcg ccggacgctg cgatcactcc
     4081 gacctcgctc tcgggcaagc tcgaggtgcg tggaagcgtc gagctcgcgc gcgtcgcgaa
     4141 cgccttgcgc tcggcgggtg cgcccctcga gcgagccagc gggcacctgg acatcgacct
     4201 cgatgcagag cgcgctccgg gcgggccgct catgcctcgg gccgagctcc gagtgaaaac
     4261 caaatcgttg aagctcgtgg agcagcgccc tccgaagaag ggggtctccg acgcgagcga
     4321 cgcccgagca acgcagccgc gctcgctcga cggcgtcgac gtggacgcca cggtctcgct
     4381 cgacccccag gaaaacaagg cgaaggtgct cggttccctg ttcgaccaaa gggggccgat
     4441 gctcgagctc gacgcggaga cgcacctgcc gaagcagggg cttccgctcg gcgcggcgct
     4501 ctccgcgact acggtcaagg tttcggcgcg catccccgct cgtgagctcg accaattgcc
     4561 gcgcagcgta cgtccgccct cgatgcacgg cgtggtgcag gcgaacgtag aggccgaagg
     4621 caacctgcgc tccccgaagc tgatggcgac ggcgggggtt cgcgggtttc ggactcgaga
     4681 aggcgggcgc cgcgccatga acgcggagct catgttgcgt tacgacaaag ctcgcggcga
     4741 gctcaggggc agcgcgagca catcgaggca gcccaacgca ctgttcgtcg acacgcgctg
     4801 ggacggcgat ttggtcggca agttcatgga cgagaacgcg ccgctcgacc tcgacgcgga
     4861 ggtgaagctc gaccggtttc cgactggcgt cgtgccgatg ctcgccgacc gttcggtgaa
     4921 aggccccgtc agctgcgaca tcaagctcga agactggggc aaggacgcga agctcaccgg
     4981 gactctcgac ggcagcggga tgaagctcag cggcgtgcag ctctcgaagc tcgatgtgtc
     5041 cttcaacacc gaaaacgagc gcatcagcgc gctcgtcgac gtgcgggagc aggacggcac
     5101 gctgcggctc gacgcgagca cgccactcac ctgggggcac tcgatcgccc cgaccgtgga
     5161 cccgcacctg aaggcgaacc tcaaagctcg gcgcttccag ctcgatacga tgagtccctt
     5221 cgtctcgcag tacctgagca cgctggaggg cgagctcaac gccgatttcg gcctcgagat
     5281 ggggcaggac gcgccgaagc tccagggctc cgcggagatc agagacggcg tcgtgcaaat
     5341 tccccaggtc gggcagcgat tttcggacgt ccaggccaag ctcagcgtcg acccgaacgg
     5401 taaagtgcgc ttggagaagc tgcaagcgcg cggcacgagc gggcgcgtga cggccgatgc
     5461 cgaagcaaca ctgcgcggca ccaacgtcga atccgcaaaa ctcgcggccc gcatcacgaa
     5521 ggcggagaag cttcctgtca ccctggaggg cgtcgcgctc ggcgacgcgt ggggcaagct
     5581 caacgtgaac taccgccacg cagacggtac caacgacatt cgcgtcgaca tcccagagtt
     5641 tcagatgcaa atgccggaag cagggttcgg caacgtgcag gggctcgacc ccgacgaaca
     5701 catccgcatc ggggtctacc gcggcgacaa gacgtttgct gcgatcccga cgcagccgct
     5761 gcgcgcgagc gagtcggaca cgccgcccgc cgagcctcca accgtgacgc gagtcgccgt
     5821 gcacctcggc gactcggttt ggatccagcg cggcacccaa gccaaggtgc agctcggagg
     5881 cgagctgaac atcacttcgg gtgaagaaag ccagattcgc gggcgaatcg atctgcgcgg
     5941 cggccaactc gacgtcaacg gcaagacctt caacatcgag aacggaacgg tcaccttcga
     6001 gggcgacgac ccgggcaacc cgaccatcgt cgcgaccgct cgctgggacg caccggactc
     6061 gtactcggtt tacgcggagt accggggcac ggtgaaggct ggtaagctga cgctgcgttc
     6121 ggaaccaccg ctcacccagc ccgagatcgt gagcctgctt ctgttcggca gtcccgaggg
     6181 ctccctcggc cagagcaacg gtggcggtgg gggtgcggcg gcgacggcgg tgggtgttgc
     6241 cggcgacacg gcggtgcgcg gcttcaaccg cgtgatgagc gacttcacgc atctggacgt
     6301 cagcgcccgc atcgatacga gcacgggcag cgcccgcccc gagatcgtcg tccaagtgac
     6361 tccgagactc accacccggg tcacgcgagc cctcggcgaa ccacccccgg gagaaagccc
     6421 ggatcgcacg ttcctcactc tggagctacg tctacagcgc tcatgggccg tgtccgcggt
     6481 cgtcggagac cgcggcgcct ccgtg
//
LOCUS       DASTKS010001213         7509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1983237, whole genome shotgun sequence.
ACCESSION   DASTKS010001213 DASTKS010000000
VERSION     DASTKS010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983237"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFQ35_25875"
     CDS             <1..675
                     /locus_tag="VFQ35_25875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934168.1"
                     /translation="LGFILLVAGVAFVAGLAVKNAKQGDAAAPAIPAAAPLAEAKPVE
                     PLPQPVAQVETPAPPAPPTEQPSETPAVASPPPALAAPENEPRRSPTPTVHAAKVSSA
                     EQAPRPSTQREAPPSTVLPVRSANVAAEAPPVEAAFDSEATTTALNAAAQRASSCRKQ
                     SDPSGVAVVTITFAPSGRVTSANISGPPFAGTETGSCIASVLRGVKVPAYSGDFMTVR
                     KTIQVE"
     gene            complement(758..1096)
                     /locus_tag="VFQ35_25880"
     CDS             complement(758..1096)
                     /locus_tag="VFQ35_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9934169.1"
                     /translation="MKLIMAIIRPHKLEEVKAALSEAGILGMSVSEIRGFGRTGGKTE
                     VYRGSAYQVDFVPKMRVDLVVADNQVHSILDALERSAKTGKIGDGKVFVLNVEEAMRI
                     RTGERGDDAI"
     gene            complement(1120..2565)
                     /locus_tag="VFQ35_25885"
     CDS             complement(1120..2565)
                     /locus_tag="VFQ35_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9934170.1"
                     /translation="MKAADRVRKRLHSVALATLALVAPVVALADEAPAAAPPDLAARV
                     ADLEAYVTNGAPKALISAGPGHNAWMMTSAALVLFMTLPGLALFYGGLVRRKNVLSVL
                     AQCLGCAGVVTILWWAIGYSLVFAPGSPLLGGGKFAMLAGVTSAPNADYGAWVSQNVF
                     SMYQLMFAIITPGLIVGAIAERMKFAAIMAFIVGWMFIVYFPLAHMVWGIDGLMNGVW
                     NAKAGIKAIDFAGGTVVHMSSGWSALVLCLLLGKREGFGARPFLPHSLVLTMVGTGML
                     WVGWYGFNAGSAVAADGIAAQAFTTTTLATAVACTVWPALEWLTRGQPTVLGFCSGAV
                     AGLVVITPSAGFVSSTGAVIIGVLAGAVPFFACTKLKAALKYDDALDTFGVHGVGGTM
                     GALLTGFFATPDANGNLNTNLKDIVGKTLWLEQLKAMGLTIVLAVVATVVLAYAVKAV
                     IGLRPDKEQEEAGLDTTDHGESGYHYEEAGG"
     gene            complement(2816..3712)
                     /locus_tag="VFQ35_25890"
     CDS             complement(2816..3712)
                     /locus_tag="VFQ35_25890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9934171.1"
                     /translation="MCRAVASFVAGDAAQARARLEAVLAAHPEQLSALAVQAQMLARS
                     GQREAALGLLDRVIARFPDYPGAQALLGALLLPGPHYRDVLAKLHSELAPRAYLEIGV
                     DTGATLALAGACNVAIGIDPAEIRPAKPLPACVRLFRATSDAFFAAHTADEVLGSRRL
                     DFVFIDGMHRFENALADFDHAEGWSNPSGTIVFHDCLPLHPVTASRERRSSFWVGDTW
                     KVVIALRSARPELRIRTIPCAPSGLVVVRGLNPDSKILSARREELLQRFGAIGWERPL
                     GDFPPDFSLVGNDATGIRAAIT"
     gene            complement(3883..4272)
                     /gene="apaG"
                     /locus_tag="VFQ35_25895"
     CDS             complement(3883..4272)
                     /gene="apaG"
                     /locus_tag="VFQ35_25895"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET9934172.1"
                     /translation="MSSSEAVTRGVRVHVEARFSPNHSDPKKREWFFLYTIQIKNEAS
                     STVQLINRHWVITDAHGHVEEVRGPGVVGKQPVLKSGESFEYTSGCPLKTPFGSMRGS
                     YEMVSESGDRFDAAIAPFALRENEAFN"
     gene            complement(4423..5565)
                     /locus_tag="VFQ35_25900"
     CDS             complement(4423..5565)
                     /locus_tag="VFQ35_25900"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934173.1"
                     /translation="MQFVADSRALEQACRALAEHRVFYLDTEFDSLREGTSLCLIQAS
                     AGGEIFLVDALSLSDLSPLGAVLGQPEATWVLHAGQQDVPLLRDRLHVEPPRVFDTQI
                     AWSLVSVEYSTSLAYLKYRLLGLRGEKAHQADDWRRRPLPQAQLAYAADDVAHLPAIY
                     AELVQRCARSGRLTAAFEASSEMLRPAVEPPQPLRLEAFRNAWQLEPEGQAVLRFLID
                     WYNRLEEEEREIAPDAKGLFSVASRRPRSFEELSSLRAVPRRAANRFGRALIAGIQQA
                     LARANASDFPEIAPPPYATAAEILAAGWLEAIRAEVCAEHRMAPELVLPSRVMKKLRD
                     GAVAAGNLTGALDALTGFRRELLLEALRAKMGGYGSLPAPTEPESS"
     gene            5709..6368
                     /locus_tag="VFQ35_25905"
     CDS             5709..6368
                     /locus_tag="VFQ35_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9934174.1"
                     /translation="MAACYFDVDGTLVGTNLVQPTLYYLANQGTPLQSAMRLGRALVG
                     APRMAVAELFDRRTFNELLFSTYAGMSEDRLELLGEDTFRQVMLPSLYPSAKGLVEKS
                     LAAGHEVVFVSGALECVVRHLARHLGATQVIANRLEMKEGIATGKLLRPVVAGPEKAR
                     IVRDHARSRGFDLDECFAFSDSYSDVPMLSVVGHPAAVNPDKRLAMLAKAYSWPSFDL
                     R"
     gene            6382..>7509
                     /locus_tag="VFQ35_25910"
     CDS             6382..>7509
                     /locus_tag="VFQ35_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9934175.1"
                     /translation="MNHPCLVTLERDSAPRVLFSGDQLVEVDLPPGTRCIYPKPALAP
                     IKDPEAAIRYAINHPAGSEPLYAKLRPGMKVTIAIDDISVPLPPMRRPDVRERVLTVV
                     LQLLADHGVDDVEMIIATGIHRRMKAQEVRHMVGDKIFEAYWPERLYNHDAEDTKNMV
                     EVGRTDHGEHLWLNRRAVESDLLIYVNVNFVAMNGGYKSVAVGLCNYESLRPHHNPHT
                     IRASDSYMDPKASRLAHDIARMGRLAHKKLNVFTIETTVNNRMFDKNLDFLYKNEDDL
                     TGKERLLMKALIASTAKLPQPARQAIFDKFPAPYELTGVFAGSAEAVHERAIEKCFDQ
                     YLVPIEGQADVLVTGIPYISPYNVHAFLNPLLVQVLAQGYLF"
BASE COUNT         1404 a         2476 c         2406 g         1223 t
ORIGIN      
        1 ctcggcttca tcctgctagt tgccggagta gccttcgtcg ccgggcttgc cgtgaagaac
       61 gcaaagcaag gcgatgccgc cgcaccggcg atcccagcgg cggctccgct cgctgaagcg
      121 aagccggtcg agccacttcc gcagcccgtc gcgcaggtcg agacccccgc tcccccggcg
      181 ccaccgacgg agcagccgag cgagactcct gcggtcgcga gcccgccgcc cgcgctcgcc
      241 gcgcccgaaa acgagccgcg gcgctccccc accccgaccg tgcacgcggc caaagtgtcg
      301 agcgccgagc aagctccgcg gccgtcgacc cagcgcgaag ctccgccctc gaccgttctg
      361 cccgtgcgga gcgcgaacgt cgcggcagaa gcaccacccg tagaagccgc attcgactcc
      421 gaggcgacga cgaccgcgct caacgctgcg gctcagcgcg cctcgagctg ccgcaagcag
      481 agcgacccga gcggggtcgc agtcgtgacc atcacgttcg ctccctccgg ccgcgtcacg
      541 agcgcgaaca tctctggccc tcccttcgcg ggcacggaga cgggcagctg cattgcctcg
      601 gtcttgcgcg gagtgaaggt ccccgcgtac tccggcgact tcatgaccgt caggaagacg
      661 attcaagtcg agtagtcgcc tccgcaacgc tcgaacgaaa aacgggaggt cagtgcgtta
      721 cgccgacctc ccgttcctca ttttgctggt ctcgcgatca gatcgcgtcg tcgccgcgct
      781 cgccggtgcg aatgcgcatg gcttcttcga cgttcaggac gaagactttg ccatcgccga
      841 tcttgccggt ctttgcgctg cgctcgagcg catcgaggat ggagtgaacc tggttgtcgg
      901 cgacgaccag atcgacgcgc atcttcggga cgaagtcgac ctgatatgcc gagccgcgat
      961 acacctcggt tttgccgccg gtgcgaccga agccgcgaat ttcgctcacc gacatgccga
     1021 ggatgccggc ttcggagagc gcagccttca cctcttcgag cttgtggggg cgaatgattg
     1081 ccatgatgag cttcatgaag tctcctctcg ccggaacact cagccgccgg cttcctcgta
     1141 atggtagccc gattcgccgt gatcggtcgt atcgagaccc gcttcttctt gctctttgtc
     1201 cgggcgcagc ccaatcaccg ccttgacggc gtaggcgagg acgaccgtgg cgaccacggc
     1261 gagcacgatg gtcaacccca ttgccttgag ctgctcgagc cagagggttt tcccgacgat
     1321 gtctttgagg ttcgtattca ggtttccgtt cgcatccggt gtggcgaaaa agccggtcag
     1381 gagcgcgccc atggtcccgc ccacgccgtg aacgccgaag gtatcgagtg cgtcgtcgta
     1441 tttgagggcg gccttgagct tggtgcaagc gaagaagggc actgcgcccg cgagcacgcc
     1501 gatgatgacc gctccggtcg acgaaacgaa gccggcactg ggagtaatca ccacgaggcc
     1561 cgcgaccgca cccgagcaaa agccgaggac ggtgggctga ccgcgcgtga gccactcgag
     1621 ggcgggccac acggtgcacg cgacggcggt agccagcgtc gtggtcgtga aggcctgcgc
     1681 cgcaatgccg tccgccgcca ccgcgctgcc agcgttgaag ccgtaccagc cgacccacaa
     1741 catgcccgtg ccgaccatcg ttagcacgag gctgtgcggc aggaacggcc gcgcgccgaa
     1801 gccctcgcgc tttccgagaa gcaagcacaa gaccagcgcg gaccaaccgg acgacatgtg
     1861 aacgacggtg ccgcccgcga agtcgatcgc tttgattccc gccttggcgt tccaaacgcc
     1921 gttcatgagg ccgtcgatgc cccagaccat gtgcgcgagc gggaagtaga cgatgaacat
     1981 ccatcccacg atgaaggcca tgatcgcggc gaatttcatg cgctccgcga tcgcgcccac
     2041 gatgaggccg ggggtgatga tcgcgaacat cagctgatac atcgagaaga cgttctggga
     2101 gacccaggcg ccgtagtcgg cgttgggcgc ggaggtgacg ccggccaaca tcgcgaattt
     2161 gcctccgccg agcagtggcg agcccggcgc gaacaccaag ctatagccaa tcgcccacca
     2221 gaggatggtg acgacgcctg cgcaccccaa gcactgcgca agcacgctga gcacgttctt
     2281 gcggcgaacc aggccaccgt agaacagcgc gagaccgggc agcgtcatga acaacacgag
     2341 cgccgccgac gtcatcatcc acgcgttgtg accaggcccc gcggagatga gggccttcgg
     2401 agcgccgttc gtgacgtaag cctccaaatc cgcaactcgc gcggcgagat cgggaggggc
     2461 cgctgcgggt gcttcgtctg ccagagcaac aactggcgct acgagcgcaa gagtggccag
     2521 cgccactgaa tgtagccttt tgcgaacgcg gtcggcagcc ttcatgagac cttccttttc
     2581 ccgcgtccta tgactcataa agaaggaaga gagcctgcgg atcgcggaga cgcttgcctg
     2641 agtacgaagg cagagctttc ggctctgttt cgcacccatg tcatttcgtg tttcgcacgg
     2701 gcgcgtcttt tgcgctgaaa gaaagctgca gcgcccgtcg ctcgtctcga gcgcggaacg
     2761 aacacggaga gtcctagttc acccgcacga gtgtcgcgaa gctcgcgaat gagctctatg
     2821 tgatcgcagc tcggatccct gtcgcgtcat taccgaccag cgagaaatca ggcggaaaat
     2881 ctccaagcgg acgctcccag ccgatagcac cgaagcgctg caagagctct tctcgccgcg
     2941 ccgagaggat cttggaatcg ggattcaatc cgcgcacgac gacgagcccc gagggcgcgc
     3001 acgggatggt gcgaatacgc agctcggggc gagcactccg aagcgcgatc acgactttcc
     3061 acgtgtctcc gacccaaaag ctgctgcgtc gttcgcgcga cgccgtgact ggatgcagag
     3121 gcaagcagtc gtgaaagacg atcgtcccgg acgggttcga ccacccctcg gcatgatcga
     3181 aatcagcgag tgcgttctcg aaacggtgca tgccgtcgat gaacacgaaa tcgaggcggc
     3241 gcgagccgag cacttcatcg gcagtgtgcg cggcgaagaa tgcgtcgctc gtcgcgcgaa
     3301 aaagacgcac gcacgcgggc aggggcttcg cgggcctgat ctcggcgggg tcgatcccga
     3361 tcgcgacgtt gcatgcgcca gcgagcgcga gcgtcgcgcc cgtgtccacc ccgatttcca
     3421 gatatgcgcg cggcgcgagc tcggagtgga gctttgcgag aacgtcgcgg tagtgcggtc
     3481 ccggtagcag aagtgcgccg agcagtgcct gcgcccccgg gtagtccgga aagcgcgcaa
     3541 tgacgcggtc gagtagcccg agcgcggcct cgcgttgccc ggagcgcgcg agcatttgcg
     3601 cttggaccgc gagcgccgag agctgctcgg ggtgagcggc cagcactgcc tcgagccggg
     3661 cgcgcgcctg agcggcgtcg ccggcgacga agcttgcgac ggctcggcac aacgcgatag
     3721 caggtgcgtc gagctcgctg aaggcgtcac acagggcgat cgcttggtcc caggcgccgg
     3781 cctggaggta ttgctgaact acgaagacga tccccgccgc gtcctctccg gcgaacgcgc
     3841 ggcgcagcgc ttcgggctcg gcgacgaacg cgtcgggggt cctcagttga aggcctcgtt
     3901 ttcacgcagc gcaaacggcg cgatggcagc gtcgaagcga tccccgctct cactgaccat
     3961 ctcgtagctg ccgcgcatgg atccaaacgg cgtcttcaga ggacagccgg acgtgtactc
     4021 gaagctctct ccgctcttca aaacgggctg cttgcccacg acgccgggcc cccgaacctc
     4081 ttccacgtga ccgtgcgcgt cggtgatgac ccaatgtcga ttgatgagct gcaccgtcga
     4141 gctcgcctcg ttcttgattt gaatcgtgta gagaaagaac cactcgcgct tcttggggtc
     4201 ggagtggttg ggcgagaaac gcgcctcgac gtggacgcgg acgccgcggg tgacggcttc
     4261 ggagctagac acgcgcctaa tctagctaga cgacgaggcg cacgcgagcc cgaccgagaa
     4321 acttccccgg tttgcccctc gagctcgcgg cttcgagcgt caacgggagc gcccggccgc
     4381 tcgggcctcg gcaaagctcc accccacgcg agccgtggcg attcatgagg actcgggttc
     4441 cgtcggagca ggcaagctcc cgtagccgcc catcttggcg cgcagggctt cgagcaggag
     4501 ctcccgacgg aagccagtga gcgcgtcgag agcaccggtg agatttccgg ccgcgaccgc
     4561 gccgtcgcga agcttcttca tgacccgcga gggcaggacc agctccgggg ccattcgatg
     4621 ctcggcgcaa acctcggcgc gaatggcctc gagccagccg gcggcgagaa tttctgcggc
     4681 ggtagcgtac ggcggcgggg caatctcggg aaaatcagag gcgttggcgc gcgccagcgc
     4741 ttgctggatt cccgcaatca gagcgcgacc gaagcggttc gcggcgcgac gaggcacggc
     4801 acgcaagctc gaaagctcct cgaacgagcg ggggcgtcgg cttgcgaccg agaacaaccc
     4861 cttggcgtcc ggagcgatct ctcgctcttc ttcctcgagg cgattgtacc aatcgatgag
     4921 gaaccggagc accgcctgac cctcgggctc gagctgccaa gcgttgcgaa acgcctcgag
     4981 acggaggggc tgcggcggct cgactgcagg gcgaagcatt tcggaggatg cctcgaaagc
     5041 cgcggtcaaa cgcccgcttc gcgcgcagcg ctggacgagc tcggcataaa tggcgggtag
     5101 atgcgcgacg tcgtccgcgg cgtaggcgag ctgggcttgc ggcaagggcc gtcgacgcca
     5161 gtcgtccgct tggtgggcct tttcgcctcg caacccgagc aggcgatact tcaggtaggc
     5221 gagcgaagtg gagtactcga ccgacacgag cgaccaggcg atttgcgtgt cgaatacacg
     5281 tggcggctcc acgtgtagac gatcccgaag caaaggaacg tcttgctgcc ccgcatgcag
     5341 gacccaggtc gcctccggct gacccagcac tgccccgagc ggagaaagat cgctcagcga
     5401 gagcgcgtcc acgaggaaga tctcaccgcc ggcagaagcc tggatgaggc acagcgaggt
     5461 gccctcgcga agcgagtcga actcggtatc gagataaaag acgcggtgct ccgcgagcgc
     5521 ccggcaggct tgctcgagtg cgcgcgagtc ggcgacgaac tgcatggggg cagaggtgct
     5581 agcgcgtttt cgttcgctgc cgtgccgctt taagctggtc agccacccgg tcagcatggt
     5641 aacacgcggg ccgcatcgtc ccttcgctgc ttgacacttt catcgaccta tacgaccgag
     5701 aaccccccgt ggctgcttgt tacttcgacg tggacggcac gttggttggg acgaacctgg
     5761 tccaaccgac tctctactac ttggcgaacc agggcacgcc gcttcaaagc gcgatgcggc
     5821 tcggccgcgc gctcgtcggc gcgccgcgca tggcggtcgc ggagctcttc gataggcgaa
     5881 ccttcaacga gctcctgttc agcacctacg cgggcatgag cgaagaccgg ctcgagctcc
     5941 tcggtgaaga tacttttcgg caggtcatgc tgccgtcgct ctacccgtcg gccaaaggtc
     6001 tggtcgagaa gagcctcgct gccggccacg aggtagtttt cgtttcgggc gccctcgagt
     6061 gcgtcgtgcg ccacctggct cgtcacctcg gcgctaccca ggtgatcgcg aaccgcctcg
     6121 aaatgaagga aggcatcgcc accggcaagc tcctgcgccc ggtcgtcgct ggaccagaaa
     6181 aggcccgtat cgtccgcgac cacgcccgct cgcgagggtt cgacctcgat gagtgcttcg
     6241 ccttcagtga cagttactcc gacgtcccga tgctcagcgt cgtcggacac cctgcagcag
     6301 taaaccctga caagcgcttg gccatgttgg caaaagcgta cagttggccg agcttcgacc
     6361 tccgttgaac cgcgcttccc gatgaaccac ccctgcctag tcaccctcga gcgcgatagc
     6421 gcaccccgcg tcctcttctc cggcgatcag ctggtggagg tcgacctgcc gcccggaacg
     6481 cgctgcatct atccgaagcc ggcgctcgcc cccatcaagg atcccgaggc ggcgattcga
     6541 tacgcgatca accatcccgc aggctcggag ccgctctacg cgaagctgcg gccgggcatg
     6601 aaggtgacca tcgcgatcga tgacatctcg gtgccgctgc cgccgatgcg ccgccccgac
     6661 gtgcgtgagc gcgtgctgac ggtggtgctt cagctgctgg cggatcacgg cgtcgacgac
     6721 gtcgagatga tcatcgcgac cgggatccac cgccgcatga aggcgcagga agtgcgccat
     6781 atggtgggag acaagatctt cgaggcgtac tggccggagc ggctctacaa ccacgacgcc
     6841 gaagacacga agaacatggt cgaggtcggt cgcaccgatc acggcgagca cctgtggctg
     6901 aaccggcgcg cggtggagag cgatctcctc atttacgtga acgtgaactt cgtagccatg
     6961 aacggcggct acaagtcggt cgcggtcggc ctctgcaact acgagagcct gcgaccgcac
     7021 cacaacccgc acacgattcg cgcgtccgac agctacatgg acccgaaggc ctcgcgcctc
     7081 gcgcacgaca tcgcgcgcat gggacggctc gctcacaaga agctcaacgt gttcaccatc
     7141 gagacgacgg tgaacaaccg tatgttcgat aagaacctcg actttctgta caagaacgag
     7201 gacgacctca cgggtaaaga gcggctcctg atgaaggcgc tgatcgcctc gaccgcgaag
     7261 ctcccccagc cggcgcgcca ggccattttc gacaagtttc ccgcgcccta cgagctgacc
     7321 ggcgtcttcg cgggttcggc cgaggcggtg cacgagcgcg cgatcgaaaa gtgcttcgat
     7381 caatacttgg tgccgatcga gggtcaagcc gacgtgctcg tgacggggat cccctacatc
     7441 agcccgtaca acgtgcatgc cttcttgaac ccgctcttgg tgcaggttct cgcgcagggc
     7501 tacctcttc
//
LOCUS       DASTKS010001214         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_864236, whole genome shotgun sequence.
ACCESSION   DASTKS010001214 DASTKS010000000
VERSION     DASTKS010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864236"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFQ35_25915"
     CDS             <1..293
                     /locus_tag="VFQ35_25915"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9934176.1"
                     /translation="KKFPLIKFSSTKVVKAGKGYKVTGNLTIRDVTKPVTLDATLSAP
                     VKNPWGKQVRAATLTGKIKRADFGLTWNKALETGGVVVGDVVTLEIQAEVTR"
     gene            complement(353..1153)
                     /locus_tag="VFQ35_25920"
     CDS             complement(353..1153)
                     /locus_tag="VFQ35_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934177.1"
                     /translation="MTSRKPSWFHALVLVASVSTSCAGGGPSVVQTRPEFRVGAHRIG
                     VVGLFRNGRLDQSTWQVIASRVVSPLGSTHCDSAFSRALREAEPDTYAELDRRIKEEG
                     LNNEALALLVPYTEADLLLIVDGRDPKRRAPRERKEPTDLPGRPVRSGHARNSTHRSE
                     PDQPTVATGTGYALTASLYSVSLQKLVARIDTHDSVTIEGAADELARNMAPLVAGSSC
                     ARWKWLDHWAAPDQPAAPAPSGGNSEASSAPDAATARDPAGQAGAPGE"
     gene            complement(1249..2235)
                     /locus_tag="VFQ35_25925"
     CDS             complement(1249..2235)
                     /locus_tag="VFQ35_25925"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9934178.1"
                     /translation="MKAAPAADEPSRRGRIAGIPLEVSRLIQGTLLMSQLAEEQAFEL
                     FDAIYSAGCNAFDTALVYGAGRAEQLLGRWARLRGVRSSIVIIDKGCHPAGETPRMTP
                     EALRRDVQRSLENLDSEYVDLYLLHRDDPSLPVGEIVSALAEEQRVGRIRSYGGSNWS
                     HERLQQANDYAARHDLPRFAASSPALSLVVPVRSWPGCVSVAHDTAALEWYRASQMPL
                     LAWSPLASGFLSGRFQRGDALERASAEERRVLDFYASDANWTRLERLKALARRKAVSP
                     AAVALAYVLHAPLDLYAVLGCREIEEYLGALASLRVRLSTEELAFLDASGRA"
     gene            complement(2232..3026)
                     /locus_tag="VFQ35_25930"
     CDS             complement(2232..3026)
                     /locus_tag="VFQ35_25930"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9934179.1"
                     /translation="MEFRGRWVLLTGASSGLGHEMAKMLAERYGANLIIVARRAERLA
                     ALKSELEERARIQVVTCSADLSKLEDVDRVIREANGHELYAAILNAGVTHFGSHRSLD
                     WDAFRQMLDTNVTAVVRMSTALIPELEKRGGGLMVVSSMAGIHPVPYQTAYSATKAFL
                     VHFGAGLWHELQGRNVSVTTYAPSGVVTEMTAGENFNPLRRWLMPVEQAALEGIEAFR
                     QRKYVHIPGFANRVGSVFARMLPQRFATGLVAAQYRNALVKQGKDV"
     gene            complement(3099..>3472)
                     /locus_tag="VFQ35_25935"
     CDS             complement(3099..>3472)
                     /locus_tag="VFQ35_25935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934180.1"
                     /translation="GSEGGAGGAAGAAGGGGAGEAGEAGAAPLGGSGGSGGSGATGGT
                     SMSGGSAGTSGGAMSGGTMNGGSSGSPSGAGVAGDDHVVILGGGLCSYQPARVATNGA
                     WILAAMVSASALRRRRNRRSR"
BASE COUNT          611 a         1182 c         1134 g          545 t
ORIGIN      
        1 cgaagaagtt cccgctcatc aagttctcgt cgaccaaggt cgtcaaggct ggcaagggct
       61 acaaggtgac gggcaacttg acgatccgcg acgtcacgaa gcccgtcacg ctcgacgcca
      121 cgctgagcgc gccggtcaag aatccgtggg gcaagcaagt acgcgccgca acgctgaccg
      181 gcaagatcaa gcgcgccgac ttcgggctca cctggaacaa ggcgctcgag actggcgggg
      241 tcgtcgtcgg agacgtcgtc accctcgaga tccaggccga agtgacgcgc tgagcggtca
      301 ccgttcgggc ccagggcccg cgaatgagta tgacttaccc attcgcgggg ccttactcgc
      361 ccggcgcgcc cgcttggccc gcagggtcgc gagcagtggc agcgtcgggc gcgctcgacg
      421 cctcggagtt cccccctgac ggcgccggtg cggcgggttg gtccggggcc gcccaatgat
      481 cgagccactt ccagcgcgcg caagacgagc cggcgacgag cggagccatg ttgcgggcga
      541 gttcgtcggc ggcgccttcg atggtcaccg agtcgtgagt atcgatgcgc gcgacgagct
      601 tttgcagcga cacggagtag agcgacgcag tgagcgcata gccggtgcca gtcgcgaccg
      661 taggctgatc cggctccgag cgatgcgtgg agttgcgtgc atgaccgctg cgcaccggcc
      721 gtcccggcag atcggtgggc tcctttcgtt cgcgcggggc gcgacgcttc gggtctctgc
      781 cgtcgacgat gagcaagagg tcggcctccg tgtacggaac caagagagcg agcgcctcgt
      841 tgttgagccc ctcttccttg attcttcgat cgagctcggc gtaggtatct ggctcggcct
      901 cacgcaacgc gcgactgaac gcgctatcgc agtgagtact cccgagaggg ctcacgacgc
      961 gggaggcaat cacttgccac gtagactgat cgagacgccc attccggaac aggccgacga
     1021 cgccgatacg atgggcgccg accctgaact ccgggcgagt ctggacgaca gacgggccgc
     1081 cgccagcgca gctcgtcgag acggacgcga ccagcacgag tgcatggaac cagctcggtt
     1141 ttcgagacgt catctggagc gccctctatt agaaccgtcc gcagtctccc gcaagcgcgt
     1201 ggccaaaccg gaacggcgat gcccggacta cctctcgcga gcgaacgctc aggcgcggcc
     1261 cgacgcatcg aggaaggcga gctcttccgt ggagagcctg acgcggagcg acgcgagcgc
     1321 gcctaggtac tcctcgattt cgcgacagcc gagcacagcg tacaggtcga gcggcgcatg
     1381 cagcacgtaa gcgagcgcga ccgcggcggg tgagaccgct ttccgccgcg cgagcgcttt
     1441 gagtcgctcg agtcgagtcc agttcgcatc cgaggcatag aaatcgagca cacgacgttc
     1501 ctcggccgaa gccctctcca aagcgtcgcc tcgctggaat cgacccgaga ggaaacccga
     1561 ggccagcggt gaccacgcga ggagcggcat ttggctcgcg cgataccact cgagcgccgc
     1621 ggtatcgtgc gcaacgctga cacacccagg ccaggaccgg accggcacga ccaagctgag
     1681 cgccgggcta ctagctgcaa atcgaggaag gtcgtgtctc gccgcgtaat cgttggcttg
     1741 ctgcagtcgc tcgtgagacc aattcgagcc gccgtacgag cggatacgcc cgacgcgctg
     1801 ctcctcggcg agcgcgctca caatttcacc aacgggcagg ctggggtcgt cgcggtgcaa
     1861 caagtaaagg tcgacgtact cgctgtcgag gttctcgagc gagcgctgga cgtcgcgccg
     1921 caacgcctcg ggagtcatgc gaggcgtttc ccccgcgggg tgacagccct tgtcgatgat
     1981 tacgatcgaa gagcggactc cccggagacg cgcccaacgc ccgagaagtt gttcggctcg
     2041 ccccgcgccg tacacgagcg cagtgtcgaa ggcgttgcat cccgccgagt agatcgcgtc
     2101 gaagagctcg aacgcctgct cttcggcgag ctgactcatg agcagcgtgc cctgaatcag
     2161 gcgagatact tccagcggga tgccggcgat acgaccgcgc ctcgatggct cgtccgccgc
     2221 cggggccgcc ttcaaacgtc cttaccctgc ttcacgagcg cattccggta ctgcgcggcg
     2281 acgagcccgg tggcgaagcg ctgcggcagc atgcgggcaa acacgctccc aacgcgattc
     2341 gcgaaaccgg gaatgtgcac gtacttgcgc tgccggaaag cctcgatgcc ctcgagcgcg
     2401 gcctgctcga cgggcatgag ccacctgcgg agtggattga agttctcccc cgccgtcatc
     2461 tcggtcacga cgccgctcgg tgcgtaggtc gtcaccgaga cgttgcgacc ctgcagctcg
     2521 tgccaaagcc cggccccgaa gtgcacgagg aaagccttcg tcgcggagta agcggtttgg
     2581 tacggcactg ggtggatacc ggccatactg gagacgacca tcaacccgcc gcctcgtttt
     2641 tcgagctctg ggatcagcgc cgtcgacatg cgtacgaccg ccgtcacgtt cgtatcgagc
     2701 atctgacgga acgcatccca gtcgaggctc cggtgggacc cgaagtgcgt tacgccggcg
     2761 ttcaggatcg ccgcgtagag ttcgtgacca ttcgcctctc gaatgacgcg gtcgacgtct
     2821 tcgagcttgg acagatccgc gctacacgtc acgacttgga tgcgcgctcg ctcttcgagc
     2881 tcgctcttca acgcggcgag gcgctcagcg cggcgcgcga cgatgatgag gtttgcgccg
     2941 tagcgctccg ccagcatctt cgccatctcg tgtccgaggc cggacgaggc gccagtgaga
     3001 agcacccagc gcccgcgaaa ttccatcggt tgcatgctgg aagggggcgt agcggacgcc
     3061 ccccgagact gtcaactccg atcgaattcc gggccggctc agcggctccg gcgattacgc
     3121 cggcgacgca aagcgctcgc gctcaccatt gcggcgagaa tccaagcgcc gttggtcgcg
     3181 acgcgcgcgg gctgatagga gcacagcccc ccgccgagga tcacgacgtg atcgtccccg
     3241 gcgacgccgg cgccactcgg actgccggag ctgcctccgt tcattgttcc tccgctcatg
     3301 gcaccacccg aggtgcccgc gctcccaccg ctcatgctgg tcccgccggt cgcgccgctg
     3361 ccgccgctac cgccgctgcc accgagagga gcggcaccag cctctccggc ttcgccagcc
     3421 cctccgccac cagccgcacc ggcagcgccg ccggctccgc cttcggagcc gg
//
LOCUS       DASTKS010001215         4023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1758527, whole genome shotgun sequence.
ACCESSION   DASTKS010001215 DASTKS010000000
VERSION     DASTKS010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758527"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFQ35_25940"
     CDS             complement(<1..1123)
                     /locus_tag="VFQ35_25940"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9934181.1"
                     /translation="MQPDFSELQRQLLGAFFEESFEGLEHLESGLLSLGRGSENSAET
                     INDVFRVAHSIKGGAGSFGLHAVGELGHSMETLLDSFRAGKLSPDAESLSLLLEAVDV
                     LRELLDATRAGNTYPPGRVEALCARLTASSRKSLAAPSKPEPTAPSSPLSSKFEVHFA
                     PLPHLLTTGNEPVRLLRELSRLGSAVTRTDTTKLPELSSLDTSLCYLSFDVELTGAVT
                     REQIADVFAWVDGDAELSVREIPPQSAAEPPAVAPPQPKAKAEAEPPREAAPPGQQAA
                     RADSEAGLGSIRVSVDRIDLLMNMVGELVITQSMLGELDDGPLDARRIERLREGLGLL
                     ARNTRSLQESVMRLRSMPISMVFNRFPRLVHDLSRQLGKQ"
     gene            complement(1143..1505)
                     /locus_tag="VFQ35_25945"
     CDS             complement(1143..1505)
                     /locus_tag="VFQ35_25945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934182.1"
                     /translation="MARIITVDDSVSLRKLVASTLASAGHEVVDVGSGADALTAAKQK
                     KFDLVISDLNMPGMDGLTFVKQLRQIAAYQTTPVLILTTEMDPNKKKAAKDAGCTGWL
                     VKPFDPAQLLATIRKVLD"
     gene            complement(1524..1814)
                     /locus_tag="VFQ35_25950"
     CDS             complement(1524..1814)
                     /locus_tag="VFQ35_25950"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9934183.1"
                     /translation="MDSQDSSNRVLELPSEIGIQEAEALRLELLALVASRAQIVVSCK
                     EVQRVGTAGLQVLASLAKTLRSNGASLSLREPTTALVDAARGLGLQDLLGIA"
     gene            2036..2986
                     /locus_tag="VFQ35_25955"
     CDS             2036..2986
                     /locus_tag="VFQ35_25955"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9934184.1"
                     /translation="MDAARADNHQSGTFVQTADLTHAVNIAVAGVQENLDRARGIVRD
                     AVSGLMASFSTLRTVLEHQQTMLKDVSSSMGKVGSSNFTAAVGRMVNEFVDEVVRVSH
                     ESMRIIEQLLITSEHVGAIVARAERIDTLARETRFIALNARIETQRAGEAGKTFKVVA
                     DEVKRLAGASAELSVQIQREVDESFRSLQATQKTAESLAGHDMSTVIESRTLLVATIE
                     RLDQINKAVEGTLQQVRAAVGDAIRALQFEDMVSQLLTESARRVRRLGQLMLDAVSAA
                     EGRRADSAARIGEISRELKELGKTASVTQTSVEQGSIELF"
     gene            2998..3291
                     /locus_tag="VFQ35_25960"
     CDS             2998..3291
                     /locus_tag="VFQ35_25960"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9934185.1"
                     /translation="MTIASTREGIFCRIEVHGRFDFSCRADFRTAYTAASRGTKFIVD
                     MTSVDYVDSAALGMLLLLRDHAGGAENVVISGCKGQPDQVLKIANFHKIFRFE"
     gene            3294..>4023
                     /locus_tag="VFQ35_25965"
     CDS             3294..>4023
                     /locus_tag="VFQ35_25965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934186.1"
                     /translation="MDQNAADAIWIVGPSDGAAALERLIRNGATPSANAATCLAQLAQ
                     GQGSGHVDAVLLRIPPTDLVSELQLLKEDPWLCDIPVLVLLQSYDDALVTACTRAGAF
                     QCLVEPVSQELCEAVIDAARATQLKAHPLTLDPQQVDRIALSFRTPAEAEGAARFLAG
                     FCPQPARQELGIAEILLNAVEHGNLGIGGEQKARLIEANALHDEIFARLQDPLYSARR
                     ARVSLRRFRDHVEICCEDEGEGFDW"
BASE COUNT          719 a         1229 c         1328 g          747 t
ORIGIN      
        1 cctgcttgcc gagctgccgg ctcaagtcgt gcacgaggcg cggaaaccgg ttgaagacca
       61 tgctgatggg catgctgcgg agccgcatca cgctctcctg caaagagcgc gtgttgcgcg
      121 cgaggagccc gagcccttct cgaagccgct cgatgcgtcg cgcgtcgagg ggaccgtcgt
      181 cgagttcgcc gagcatcgac tgggtgatca cgagctcgcc aaccatgttc atcagcaagt
      241 cgatgcgatc gacgctgacg cggatggaac cgaggccagc ctcggagtcg gcgcgcgcgg
      301 cctgttgccc tggtggtgcg gcttcacgcg ggggttccgc ttcggccttt gcctttggct
      361 gaggtggcgc gaccgcgggc ggctccgccg ccgactgagg tggaatttcc cgtactgaaa
      421 gctcggcgtc gccgtcgacc caggcgaaca cgtccgcgat ttgttcgcgc gtcaccgccc
      481 cggtgagctc gacgtcgaag gacaggtagc agaggctcgt gtcgagcgaa gacagctcgg
      541 ggagcttcgt cgtgtcggtg cgcgtcaccg cgcttccgag tcgcgacagc tctcgtagca
      601 gccggacggg ctcgttgccc gtggtgagta gatggggcag cggcgcaaaa tgaacctcga
      661 atttgctgct caggggcgag ctcggagcgg tcggctccgg cttggatggc gcggcgaggc
      721 tcttccgtga gctcgcggtc aggcgcgcgc acagcgcttc gacgcggccc ggggggtagg
      781 tgttgccggc gcgtgtcgcg tccaagagct cgcgcagcac gtccaccgcc tcgaggagca
      841 gcgagaggct ctcggcgtct gggctcagct tgccggctct aaacgagtcg agcagcgtct
      901 ccatcgagtg gccgagctcg ccgaccgcgt ggagcccgaa gctcccggcg ccgcctttga
      961 tcgagtgcgc gacccgaaat acgtcgttga tcgtctcggc gctattttcc gagccgcgcc
     1021 cgagcgagag caagccggat tcgaggtgct cgaggccctc gaagctctcc tcgaagaagg
     1081 cgcccagtag ctgccgctga agttcggaaa agtcgggttg catccttctg cgctccgcga
     1141 tctcaatcga ggactttgcg aatcgtcgcg aggagctgag cggggtcgaa aggcttgacg
     1201 agccaccccg tgcagccggc gtccttggcc gccttctttt tgttgggatc catctcggtc
     1261 gtgaggatca gcacgggcgt ggtctgatac gcggcgattt gccggagctg cttgacgaag
     1321 gtgagtccgt ccatgccggg catgttcaga tcgctgatca ccaagtcgaa cttcttctgc
     1381 ttggccgcgg tcaaggcgtc cgcaccgctg cctacgtcga ccacttcgtg accagcggag
     1441 gccagcgtcg aagcgaccag cttccttaga gatacagagt cgtccaccgt gattattctg
     1501 gccatcttcg tgagtgttct ccttcaggcg attccgagca ggtcttgcag tccgaggccg
     1561 cgcgcggcgt ccactagtgc cgttgtcggc tcacgtaggg acaaggacgc accgttgctg
     1621 cgcagcgtct tggcgaggct cgccagcacc tgcaggccgg cggtgcccac gcgttgcacc
     1681 tctttgcagg agacgacgat ctgagcgcga ctcgccacga gagcgagaag ctcgagcctc
     1741 aatgcttctg cctcttgaat gcctatctcg gaaggtagct cgagcactcg attggacgaa
     1801 tcctgagagt ccatctgagg ctcagcgaac ggacacgctc tcgtcgactt taccgacacg
     1861 ccccactcgg tgagaacgat catgcttttt gcgtcgcgcc ttcagcgtcg gggtgggctc
     1921 gtgtgcgggt caaaagcttg tcgcgagttt ccggcggaaa acgcggccta cgcaaagttt
     1981 gcgctcggcc gtgtacgcta aactaacgac gtcggtggtc gctgactgag atggtatgga
     2041 cgccgctcgc gccgacaacc atcagtcggg aaccttcgtt caaaccgccg atttgacgca
     2101 tgccgtgaac atcgcggtcg ccggggttca agagaacctc gatcgcgccc gcgggatcgt
     2161 gcgcgacgcc gtttcaggct tgatggcgag cttcagtacg ctgcgtacgg ttctggaaca
     2221 ccagcagacg atgctgaagg acgtcagctc atcgatgggg aaggtcggca gctccaattt
     2281 caccgccgcg gttgggcgta tggtgaacga gttcgtggac gaagtcgtac gcgtgagcca
     2341 cgagagcatg cgaatcatcg agcagctctt gatcacatcc gagcacgtcg gagccatcgt
     2401 ggctcgtgcg gagcgcatcg acacactcgc gcgcgaaacc cgcttcattg cgctgaatgc
     2461 gcgcatcgag acccagcgcg cgggcgaggc agggaagacc ttcaaggtag tagctgacga
     2521 agtaaagcgg ctcgcggggg cgtccgccga gctcagcgtt caaatccaac gcgaagtaga
     2581 cgagtcgttt cggagcctcc aggccactca gaagacggcc gagagcctgg cgggtcacga
     2641 catgagcacc gtcatcgagt ctcgcactct gctggtcgct accatcgagc gtctcgacca
     2701 aatcaacaaa gcggtcgaag gcactctcca acaggttcgc gccgcggttg gcgatgcgat
     2761 tcgggcgctg caattcgagg atatggtgtc tcagctcttg accgaatccg cccgacgcgt
     2821 gcgacggctc gggcagctga tgctcgacgc ggtgtcggcg gccgaaggaa ggcgagccga
     2881 ctccgcggcg cgaatcggtg agatctcacg tgagctcaag gagctcggta agaccgcgag
     2941 cgtgacgcaa acgagcgtcg agcaagggtc gatcgaactg ttctgaggga ggacggcatg
     3001 acgatagcga gtacgcgaga gggaatcttc tgtcggatcg aggtgcatgg tcgcttcgac
     3061 ttctcttgtc gagccgactt tcgcaccgcc tacacggcgg cttcacgagg cacgaagttc
     3121 atcgtggaca tgacgagcgt cgattacgtc gacagcgccg cgctcggcat gctcctcctc
     3181 ttgcgcgatc acgcgggcgg cgccgagaac gtggtgatca gcggctgtaa gggtcagccg
     3241 gatcaagtcc tgaagatcgc gaacttccac aagatcttcc gattcgagta aacgtggacc
     3301 aaaacgccgc cgacgctatc tggatcgttg gcccttccga cggtgccgcc gcgctcgagc
     3361 gcctgataag aaacggtgca acgccgagcg cgaacgcggc cacgtgcctt gcgcagctcg
     3421 cgcagggtca gggttccggg cacgtcgacg ccgtgctgtt gcgcatccca ccgacggatc
     3481 tcgtctccga gctccagctg ctgaaggagg acccttggct gtgcgacatt ccggtgctgg
     3541 tgctgctcca gagctacgac gacgcgctgg tcacggcgtg tacgcgagct ggcgcctttc
     3601 agtgtttggt cgagccggta tcgcaggagt tgtgcgaagc ggtcatcgac gcggcccggg
     3661 ccacgcagct caaggcgcat cctctgaccc tggatcctca gcaggtcgac cggatcgcgc
     3721 tgtcgttcag gacgccagca gaggccgagg gcgcggcgcg cttcttggcc ggattttgcc
     3781 ctcaacccgc acgccaagag ctcggtatcg ccgagatttt gctgaacgcg gtcgaacacg
     3841 gcaacctcgg catcggcggc gagcagaagg ctcgactgat tgaagccaac gcgctccatg
     3901 acgagatttt cgcgcgcctt caagaccccc tatattcggc ccgacgcgcg cgcgtgagct
     3961 tgcggcgctt tcgtgaccac gtcgagattt gttgtgagga cgagggagaa ggcttcgatt
     4021 ggc
//
LOCUS       DASTKS010001216         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1088110, whole genome shotgun sequence.
ACCESSION   DASTKS010001216 DASTKS010000000
VERSION     DASTKS010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088110"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1929)
                     /locus_tag="VFQ35_25970"
     CDS             complement(385..1929)
                     /locus_tag="VFQ35_25970"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET9934187.1"
                     /translation="MSSKSALSLGSWRDLDGASGGSVLRLPSQHLTTHAVVVGMTGSG
                     KSGLVTVLVEEALNAGIATIAIDIKGDLPNLLLNFPSFDPALYRSWVQSGGEAGPDSS
                     IDVDAQARWLADERREALGAWSLSERDIARFSERTHLRVITPGSTAGESLHVLSSLER
                     RSPRWDTDVESARAALSAAVSLLLRLLGRDPDPAKSKEHVLLSVLAERRLVAGEHADL
                     PALMHDLQVPPVAAIGALPIEKFFKRSDRLNLAAALNTLLASPTFATWRQGAGLDISD
                     WTQPREGRTPGVIVSVAHLDDEERALVLGVILEEFLSWVRTLSGSPRLRALLVFDEVY
                     GFLPPHPANPPTKRPLVALMKQARAFGVGVVVATQNPMDLDYRALSNAGLWCIGRLQT
                     DADRERVLDGLAASLRDGEDTRELDRIVPRLANRWFVVRNAHVSDVGPILVQPRYAMS
                     WLRGPMTRSELRAAREQREQWQSPAARVPADSEVTDSSPPPQSPGEGQHATSSHDVER
                     GEPISA"
     gene            complement(1937..2872)
                     /locus_tag="VFQ35_25975"
     CDS             complement(1937..2872)
                     /locus_tag="VFQ35_25975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934188.1"
                     /translation="MEDKEVGTNAGVSGVGNDPSGDLAYQQLLPELDAVPRDELVPIT
                     VDVPSVVTLVVGSLPEILHVVGAIQKVAPELDAARIQRLRTYALALNYAHARYLSAAP
                     ARNNLDALVTEATQRFATLSTDAAALVKRGLINGEELEDLGSPAGYKSVAVALTRFAT
                     VLTASWDKIEGKCAITREELDSTLRLAELILTTVGLKEQGATKLDSATDYRLRAFFLL
                     SREYDRVRQAVTFVRWTERDADQIAPSIYTGRTARRREANVPASDPVPAATPTGDASK
                     AGTPEVPGPVPNQVPPTGLSPAKENPRADPFMRGP"
BASE COUNT          524 a         1017 c          971 g          498 t
ORIGIN      
        1 gatgctgggg agacaacttc ctcagccaac tcgggctcga aaacggtgag cgcgacgatt
       61 ggtctgcgac cagcatcatc ggcgacgagc cgggcgagat gggcgacgct ctgcacgtcg
      121 tccgcgttcc gtgagggctc cggccaattc cacgaagtgc gcatcggctg gcgtaaccac
      181 ggcacacggc cgctgtcggg cggctttacc ggcgagccgc tgcatcgccg cagcccactc
      241 ttcgagcgtg agcgcatcgt tctgacccgt acgtcgaatc gtcggcgctc cggcgtgctc
      301 gcccgcgcgg ctcgctcgcc gcaccagcga ttctgagagc gcgtaagctc cgacgagctc
      361 atcggtggcg tctttctggc taagctaggc cgaaataggt tctccacgtt ccacgtcatg
      421 actcgaagtc gcatgctgac cctcccctgg tgattgcggc ggtggcgaag agtcggtcac
      481 ttcgctatct gccggcaccc gcgcggctgg cgactgccac tgctctctct gctcgcgcgc
      541 ggcgcgcagc tcgctgcgag tcattgggcc gcgcagccac gacatggcgt agcgcggctg
      601 aacgagaatg ggaccgacgt cgctcacgtg cgcgttgcgc accacgaacc agcggttggc
      661 gaggcggggc acgatgcggt cgagctcgcg ggtgtcctcg ccatctctca gggaggcggc
      721 cagcccgtcg agcacacgct cgcgatcagc gtcggtttgg aggcgcccga tgcaccagag
      781 ccccgcgttc gacagcgcgc ggtagtcgag gtccatgggg ttttgcgtgg cgacgaccac
      841 gccgacccca aaggcacgcg cttgcttcat cagcgcaacg agcgggcgct tagtgggagg
      901 gttggcagga tgcggcggca gaaagccgta aacctcgtcg aagacgagga gtgcccgcag
      961 ccgcggcgag cccgacagcg tccgcaccca agaaaggaac tcctcgagaa tcacgccgag
     1021 aacgagcgcg cgctcttcgt cgtcgaggtg agccacgctg acgatgacgc ccggcgtgcg
     1081 cccctcgcgc ggttgagtcc agtcgctgat gtcgagacca gcaccttggc gccaggtcgc
     1141 gaacgttggt gacgcgagca gcgtgttgag cgccgcagcg aggttcagtc ggtccgagcg
     1201 tttgaagaac ttctcgatcg gcagcgcgcc gatcgcggcg acagggggca cctgaaggtc
     1261 gtgcatcagc gcggggagat cggcgtgctc cccggcgacg agacgccgct ccgcaagcac
     1321 gctgagcagc acatgctcct tgctcttggc gggatctgga tcgcgcccca gcagacgcag
     1381 caacaaggag acagccgcac tcagggcggc gcgcgcggat tccacgtcgg tatcccagcg
     1441 gggcgagcgg cgctcgagcg atgacagtac atggagggat tcccccgcag tcgaccccgg
     1501 cgtgatcacg cggaggtgtg ttcgctcgga gaagcgcgcg atgtcgcgtt cggataagga
     1561 ccaggcgccg agcgcctcgc ggcgttcgtc cgctagccac ctcgcctgcg cgtcgacatc
     1621 gatcgaggaa tcaggacccg cctcgcctcc gctctgtacc caagagcgat atagagcggg
     1681 gtcgaaactc gggaagttca gcaacaagtt gggcagatcg cccttgatgt cgatggcgat
     1741 cgttgcgatg ccggcgttca acgcctcttc gacgaggacg gtgacgagcc ccgacttgcc
     1801 ggagcccgtc atcccaacta ccaccgcgtg cgtcgtcagg tgttgcgaag gcagacggag
     1861 cactgagccg cccgaagcgc cgtccagatc tctccacgaa ccgagcgaga gtgcggattt
     1921 ggagctcatg cgaagctcat ggcccgcgca tgaacggatc cgcgcgcgga ttctccttcg
     1981 ctggcgacaa gccggtaggc ggcacctggt tcgggaccgg accggggact tccggcgttc
     2041 ccgccttgga ggcgtccccg gtcggagttg cggccggcac ggggtccgac gccgggacgt
     2101 ttgcctcgcg gcgacgcgct gtgcgcccag tatagatgga aggtgcgatc tggtcggcgt
     2161 cacgctcggt ccaccgcacg aacgtcaccg cctgccgaac gcgatcgtac tcgcgggaga
     2221 gcaggaagaa cgcgcgcagc cgatagtcag ttgccgaatc tagcttggtc gcgccctgct
     2281 ccttcaagcc caccgtagtc aagatgagct ccgccaggcg caacgtcgaa tcgagctcct
     2341 cgcgcgtgat ggcgcacttg ccttcgatct tgtcccagct cgcggtgagc acagtcgcga
     2401 agcgtgtcag cgccactgcc acgctcttgt atcccgcagg agagcccaaa tcttctagct
     2461 cttcgccgtt gatcaagccg cgcttcacca acgccgcggc gtcagtcgaa agcgtggcga
     2521 agcgctgcgt cgcctccgtc acgagcgcgt ccaagttgtt gcgcgccggt gctgcagaca
     2581 ggtaccgcgc atgcgcgtag ttcaacgcga gcgcgtaagt ccgcaacctc tgaatgcgcg
     2641 cggcgtccaa ctctggcgcg accttctgga tcgccccgac cacgtgcaaa atttccggca
     2701 gcgaccccac caccagcgtg accactgacg gcacgtccac cgtaatcggc accagctcgt
     2761 cacgtggcac tgcgtcgagc tctggcaata gctgctggta agccaaatct ccggacgggt
     2821 cgttcccgac gccactcaca cccgcgttcg tcccgacttc cttgtcttcc atggtctctc
     2881 cccttcatcg ttccacgcgc gacgacgcac tcccgatccg cgttgcgcgc gaactcggta
     2941 acgtcccaca cgaagcggag tgcacgcagc cgtaccgcca ccatgcagca ctgcccgtat
     3001 aacccctgac
//
LOCUS       DASTKS010001217         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_641007, whole genome shotgun sequence.
ACCESSION   DASTKS010001217 DASTKS010000000
VERSION     DASTKS010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641007"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ35_25980"
     CDS             complement(<1..451)
                     /locus_tag="VFQ35_25980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934189.1"
                     /translation="MPPPPAAPSAAEVVLTFLESVGRRSEAELYLRLFHKLPKESFAV
                     IAPGGPVVRHGVGAFVEQLRFLGDLDLFAPVVLGLFNPEGAAAGAERLVKRLPVVGLE
                     ATPHEMGEPDLAERVRSELRKERVPVVHFRVQEGVTFQARLQALASLS"
     gene            complement(502..1374)
                     /locus_tag="VFQ35_25985"
     CDS             complement(502..1374)
                     /locus_tag="VFQ35_25985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934190.1"
                     /translation="MLKGPVARGDRCVLEFGNIFFEVDPKVGGRVTSARVAGLEVLTT
                     ASVNAVNYGSTFWTAPQADWQWPPVAEIDSDPYAVEQGEASFTLVSPPVESSTDAVAG
                     LQVKKRFSADLVRQAILVEYTLVNTSRDPKRAAPWEITRVERGGLTFYASDSAPMRAG
                     DRPLLATSRGAGAYWFQHDERTPVHGKLNADGKGWVAHVTPQRALLIKSFEDIQQSQA
                     AVGEGEVEIYANLDATSPEAYVEVENQGAYGEIAAGAALTWAVRWYLKALPPQLVVAP
                     GSADLIALVAEAIQ"
     gene            1594..2316
                     /locus_tag="VFQ35_25990"
     CDS             1594..2316
                     /locus_tag="VFQ35_25990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934191.1"
                     /translation="MNTAPSASQNARPARPRAPKFPKLGISVLAAGLLVAASVAAQKS
                     DSQEALVRRYPFDPACPWGRVANGKGMIVRCMSEDEANSILKRAPAAPAQAAPAGALT
                     GPAPTPSASASAAPPAEAPAEADTADVGATDQKLDVTVGPVVADQGELSVGKLNQPKS
                     RYAKCITDHGGLKDKTGEVDVRFLVRSKGIAEGVSVQKRVNVSAEAARCVAEVVDRRR
                     VGVPDAPLVGATVVIKFEKLAK"
     gene            2340..>4005
                     /locus_tag="VFQ35_25995"
     CDS             2340..>4005
                     /locus_tag="VFQ35_25995"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9934192.1"
                     /translation="MESYASRIAKFQAEVEQLGQRSRLISNLRGLCFGSALILGIAAA
                     AGGKPGVTGPVSLVLLFAFIGLVMFHAKVIEALDLAERWVDVNRDATHRLEHDWPSVL
                     DNGEGLAPKDHLYAGDLDLFGRGSLFQRISVARTRFGRERLAELLLTAADPSEISRRQ
                     AAVRALVPELELRQRFEAHALGVTGTQRPRDGRSRVRVAPNPGPLLTWAESEPSLLRD
                     PISVWGARLLPPITLLSVAATLYLHVSAAFFAGALLLQLLVIVRSQRETTRVFSAVSS
                     TEGAFLRYGALLETIEQFSSEVPLLRELRNRLSTGAGAPSVAMARFRSIVGWFDLRHN
                     AVYPVVNLFTLWDLHCTLALERWQQRAGKHLRDWFDVIGWFEALSSLASFAADEPNVT
                     FPELSDAVCFEADSLSHPLLRASSRVANSVSLQHAGSALLVTGSNMSGKSTLLRAVGL
                     AAVLAQAGAPVTARRLKLSRLAVATSIQVADSLEQGVSHFYAEITKLRRVVDAATSAG
                     PVLFLLDEILHGTNSRERQLGARWVLAELIKKGSIGAISTHDQELCN"
BASE COUNT          633 a         1317 c         1354 g          701 t
ORIGIN      
        1 gcgaaaggct cgcgagcgcc tgcagccggg cctgaaaggt gactccctct tgaacgcgga
       61 agtgcacgac gggtacgcgc tctttgcgga gctcgctgcg cacgcgctcg gcgaggtcgg
      121 gctcgcccat ttcgtgagga gttgcttcga ggccgactac gggcaggcgt ttgacgagcc
      181 gctccgcgcc cgcggcggcg ccttcggggt tgaagagccc gagcactacc ggcgcgaaca
      241 gatcgagatc gccgaggaag cgcagctgct cgacgaaagc tccgacgccg tgtcgcacga
      301 ctgggccacc cggcgcgatg acggcaaagc tctctttggg gagcttgtga aacaggcgga
      361 ggtagagctc tgcttccgag cgtcgcccga cgctttccaa gaaggtgagc acgacctcgg
      421 ccgcgctcgg tgcagctggt ggtggaggca ctttcgtcgt tcgggttact gaatggcttc
      481 gttacgaccg tggcgatcga gttactgaat ggcttcggcc acgagcgcaa tcagatcggc
      541 gctgccgggc gccacgacca gctgcggcgg aagcgctttg aggtaccagc gcactgccca
      601 ggtgagcgca gccccggccg cgatttcgcc gtaagcgcct tggttttcga cctcgacgta
      661 cgcctcgggt gaggtcgcgt cgaggttcgc gtagatttcg acctcccctt cacccacagc
      721 ggcctggctc tgctggatgt cctcgaagga cttgatgagg agtgcccgtt gcggcgtgac
      781 gtgtgcgacc caacctttgc cgtccgcgtt cagttttccg tgaacgggcg tgcgctcgtc
      841 gtgctgaaac cagtacgcgc cggcgccgcg tgaggtggcg agcagcgggc ggtctccggc
      901 acgcatcggc gcggagtcgg aagcgtagaa ggtgagccca ccgcgctcga cgcgcgtaat
      961 ttcccagggg gcagctcgct ttgggtcgcg gctcgtgttc acgagggtgt actcgacgag
     1021 gatggcttgc ctgacgaggt ccgcgctgaa ccgctttttt acctgcaagc cagcgacggc
     1081 gtcggtgctg gattccacgg gcgggctcac gagagtgaac gaggcttctc cctgctcgac
     1141 cgcgtacgga tcgctgtcga tttcagcgac ggggggccat tgccagtcgg cttgcggcgc
     1201 ggtccagaag gtggacccat agttgaccgc gttcacgctc gcggtggtca acacctcgag
     1261 cccggcgacg cgggcggagg tgacgcgccc gccgaccttc ggatccactt cgaagaagat
     1321 attcccgaat tcgagaacgc agcgatcccc acgcgcgaca gggcccttca gcatggcggc
     1381 tatctccgag ccccctcggc gcgcgctccc cgtactcccg ctgacatctg cgcagagcca
     1441 cgcgctgccg aacacggcga acgcgagccg aggaattgcc gcgtcggtgg gacgggggcg
     1501 acgaacatgg cggtgggcta ccatcgtgga accgcgcccg caacggcaaa tcccctcgtt
     1561 ctcgatcggt tctcggggta aagatggcgc gcgatgaaca cggctccgtc ggcttctcag
     1621 aacgctcgcc ccgcgcgccc gcgcgcgccc aagttcccca agctcggcat cagcgtgctc
     1681 gccgcggggc tcttggtggc tgcatcggtt gcagcgcaga agagtgactc tcaagaggcg
     1741 ctcgtgagac ggtatccgtt cgatcccgcc tgtccctggg ggcgagtcgc gaacggcaag
     1801 ggcatgatcg tgcgctgcat gagcgaggac gaagccaact cgatcttgaa gcgggctcca
     1861 gccgcccccg cacaagcggc tccggcgggt gctctcaccg gtcccgcgcc gacgcccagc
     1921 gccagtgcga gcgccgcgcc tcccgcggaa gctccggccg aggcggacac ggccgacgtg
     1981 ggtgcgaccg atcagaagct cgacgtcacg gtggggccgg tggtcgccga ccaaggtgag
     2041 ctcagcgtcg gcaaactgaa ccaaccgaag tctcgttacg cgaagtgcat caccgaccac
     2101 ggtgggctca aggacaaaac cggcgaggtc gacgttcgct tcctggtgcg ctcgaaggga
     2161 atcgccgaag gggtcagcgt gcaaaagcgc gtgaacgttt cggcggaagc cgcgcgctgc
     2221 gtcgccgagg tcgtggatcg tcgccgagtg ggagtgccgg acgctccact cgtaggcgca
     2281 accgtcgtga tcaagttcga gaagctcgcg aagtaagcga gcggaaacgg aagctgaggg
     2341 tggagtcgta cgcgtcgcgc atcgccaagt tccaggccga ggtggagcag ctgggacagc
     2401 gctcgcgcct gatctccaac ctgcgtggac tctgtttcgg gtccgcgctg attttgggaa
     2461 tcgcggccgc ggccggcgga aaaccagggg ttaccgggcc agtgagcctg gttttgctgt
     2521 tcgcgttcat cggcctcgtg atgttccacg cgaaggtgat cgaggcgctc gatctcgccg
     2581 agcgttgggt cgacgtgaac cgcgacgcca cccaccgtct cgagcacgac tggcccagcg
     2641 tgctcgacaa cggtgagggg ctcgccccga aggaccacct ttacgcgggc gacctcgatt
     2701 tgttcggtcg tggctcgctc tttcagcgca tctcggtggc gcgcacgcgc ttcggtcgtg
     2761 agcggctcgc cgagctcctg ctcaccgcgg cggaccctag cgaaatttcc cgacggcagg
     2821 cggcggtgcg cgcgctcgtt ccagagctcg agcttcgcca acgcttcgaa gcgcacgcgc
     2881 tcggcgtcac cggcacgcag cgacctcgcg atggtcgcag ccgcgtgcgc gtcgccccga
     2941 acccgggtcc gttgctgact tgggccgaga gcgagccgag cttgctgcgt gacccgattt
     3001 cggtgtgggg cgcgcgcttg ctgccgccca tcaccttgct ctcggtggcg gcgactctgt
     3061 acctgcacgt gagcgccgcg ttcttcgccg gcgccctgct cttgcagttg ctcgtcatcg
     3121 tgcgctcgca gcgcgaaacg acacgcgtct tcagcgccgt ttccagcacc gaaggcgcgt
     3181 ttttgcgcta cggagcgctg ctcgaaacca tcgagcaatt ctcttccgag gtgccgctct
     3241 tacgcgagct gcgaaaccgc ctgtcgacgg gggcaggcgc tccgtcggtc gcgatggcgc
     3301 gcttccggag catcgtcggc tggttcgatc tgcgccacaa cgccgtgtac ccggtcgtga
     3361 atctctttac gctctgggat ttgcactgca cgctcgcgct cgagcgctgg cagcagcgcg
     3421 ccggcaagca cctgcgcgac tggttcgacg tcatcggttg gttcgaagcg ctctcttctc
     3481 tcgcctcgtt cgccgccgac gagccgaacg tcacgtttcc cgagctgagc gacgcggtgt
     3541 gcttcgaagc ggactcgctg tcgcacccgc tgctgcgcgc gagctctcgc gtcgcgaatt
     3601 cggtcagctt gcagcacgcg gggagcgcgc tcctcgtcac cggctccaac atgtcgggca
     3661 agagcacgtt gctacgcgca gtggggctcg cggcggtgct cgcgcaagcg ggcgcgccgg
     3721 tcacggctcg tcgcctcaag ctgtcgcgcc tcgcggtcgc gaccagcatt caagtcgccg
     3781 actcgctcga gcaaggcgtt agccacttct acgcagagat tacgaagctc cgccgcgtcg
     3841 tcgacgccgc gacgagcgca ggcccggtgc tgttcctcct cgacgagatc ctgcacggta
     3901 cgaactcgcg tgagcggcaa ctgggagccc gctgggtcct cgccgaactc atcaagaagg
     3961 gctcgatcgg cgcgatttcg acgcacgatc aggagctctg caatt
//
LOCUS       DASTKS010001218         8959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_864465, whole genome shotgun sequence.
ACCESSION   DASTKS010001218 DASTKS010000000
VERSION     DASTKS010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864465"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="argB"
                     /locus_tag="VFQ35_26000"
     CDS             complement(<1..743)
                     /gene="argB"
                     /locus_tag="VFQ35_26000"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET9934193.1"
                     /translation="MDDLIRKAAVLEEALPYIRRFHNRTVVVKYGGHAMTDEVLKESF
                     AKDICLLRYVGIRVVVVHGGGPQINRMLDRVGIKSVFNAGLRVTDDETMDVVEMVLGG
                     GVNQEIVGLICQHGGRAVGLSGRDDRFMRGKKLDKVMAKDQSGNEVAVDLGRVGEVES
                     VEAGLVDQLISNGFIPVIAPIAVDNEGKSLNVNADTAAGSIAGVLRAAKLVLMTDVEG
                     VKASDGRLLRSLDAPEAEHLIAEGVISGG"
     gene            913..2298
                     /locus_tag="VFQ35_26005"
     CDS             913..2298
                     /locus_tag="VFQ35_26005"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET9934194.1"
                     /translation="MDVLMVAAELAPYARVTAAADSIAALAKALRQLGHGVTVALPRS
                     SGFQSNGPLLARRLTPLSVEPGLDVTVLEGQLPSGVSLVLFDAPEFAADELTSESGGE
                     RVALLSRAAHALVRERKEQGKRVDVVHLHDFAAAPAAALLAQAPELAPSLLTIHSFGP
                     ERTFDPASLARLGQVVDEPRARIGKRVSPLRLGMVNAKVVTTVSPGYATELGDTALGS
                     LIQALPEPLAGVLDGVDYATYSPATDAAIESRFDAEDLSNKGRCKTALVRATGLRLDP
                     ERPLLVALLGLSDPTGHDLLVRALPRLLNLPLAVVVFGAADAERSAQLSALQQQYPGD
                     LGLLLRSDDPELRRCLSAADFTLSARSHSPAAHQELFAMRYGALPIACARGAVKDVVV
                     DIDAELETGTGFSFGELSEDELVGAVQRAVSAFGSPRFGQVRRRAMRRDLGWDRPARR
                     YLQIYKKLAGG"
     gene            complement(2274..3089)
                     /locus_tag="VFQ35_26010"
     CDS             complement(2274..3089)
                     /locus_tag="VFQ35_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934195.1"
                     /translation="MNRAFSRTLGAVWLCRSVLGWLLALPFVAAVADSGVGGLFDGDR
                     VLFEPGGLFLAELGRVSGPLLNGAAATMWPYVLAAFALRTLPQGALFQQTFAPSSGPR
                     KAFGAASARVVPLVTIAAVELLAKVALVGIVVFVEQAAHAEPARRFGLLPAAALLGAG
                     LFATTALADVRRGLLFVDPELEGAALGPALSHVRTNVLPLLGGYALRVGLGMLAVAVA
                     ARLVEWIDVGRPGGWRVLAAFALHQLVLGLVTALDAFWIRRVVSPLQPPASFL"
     gene            complement(3086..5071)
                     /locus_tag="VFQ35_26015"
     CDS             complement(3086..5071)
                     /locus_tag="VFQ35_26015"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9934196.1"
                     /translation="MNFRGRAIVEAAMGLAALGISAALLAPPSRPRGAPPEQRPAKTA
                     ERAPKLVASYTLNAELDAGKHNVKGSGTIAWQNTSRSPVRSLFFHLYLNAFKNERSVF
                     LRSPFGASRDDSRGRDFGYIDVASLTVRELGNADIWPNRQRHSPGDVDDETDIEVPLP
                     RAVEPGESLTLDLRFESQLPELMERTGYSGSFHFVAQWFPKLARLEDNGEFSHFSFHP
                     QAEFYADFGRYDVTLDVPQGFTVGATGALVSQSTAGGRKRVRYVAEPVHDFAWTAWDG
                     FQEKRFSAGGLDVRMLFPAGQEKAATLSEDTLRFALPYFERRFGSYPYPTLTVMHPPP
                     GAEASGGMEYPTLITTGGDWYTPYLGMHDVQTVVVHEFGHQWFYGILASNEARWPFLD
                     EGLNSYAEQAALGAQLPRQSLFGALGITLSGDSLFRVFAAARAQDEPVGSAASEFSSF
                     RNLGALVYSRTATLLATLERVYGKARFQRALGAYAARFRFAHPTPTDLLDVIGDELGS
                     DARTFLDTALFHRGRVDYVARDLQNTRASDPAGYFERASGREHITRQQAQPTREFVGR
                     ATVYRHGSLELPVDVLLVARDGTRKLERWDGHGAFRVFEYRGATELARVVVDPDHKLL
                     IDDDLFNNELCTSSRVLWRVHERLGYFAALALAGVSP"
     gene            complement(5079..5921)
                     /locus_tag="VFQ35_26020"
     CDS             complement(5079..5921)
                     /locus_tag="VFQ35_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934197.1"
                     /translation="MTPGDGKRRGAPPPPSRPAEAPVEDVNWDDDTVPRFDLDDHAFD
                     RVTAIPELPPELMAKQMMEATQSDEPPPPELEGALTSKTESEVPNSQLSTAPPAHESF
                     SEELVLEIDDSALVAAVPPREARRALSLELDITGLDPITGVEASNSIPPSSDPQIRKI
                     ADRYATGDFSGALVLAEGILENAPEHPEAQRYANNCRDVLTQMYSARLGALDQRVTVA
                     IPSEQIRWLSLDHRSGFLLSLVDGTSSVEELLDISGMAKLDALRILYTLREERVIALV
                     PRGA"
     gene            complement(5927..7750)
                     /gene="dnaK"
                     /locus_tag="VFQ35_26025"
                     /pseudo
     CDS             complement(5927..7750)
                     /gene="dnaK"
                     /locus_tag="VFQ35_26025"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            complement(7787..>8959)
                     /locus_tag="VFQ35_26030"
     CDS             complement(7787..>8959)
                     /locus_tag="VFQ35_26030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934198.1"
                     /translation="DRYYGKNLGRFKSKLERVFQRVTDAHDTLTRNDARADYDAYLNA
                     GRRTAALERELNDEEAYARELDAARARIEAEAQRVANTPVPGHVASSTPPAPTSDPNN
                     VRPSEPTQPRPSDPVAARPSDPAGVRPSDPAVARPSEPVAARPSVPAPAGRNDADARR
                     RALARKLGLSYPPPAPQVRSSPLPPQPPSQEGTAAPSARDRAVNDLKRRYDQRMVDAR
                     RRQVHEYLGRAESSMGEAKVVEAVNALRIAVSLAPDDPVLRRRLEDLQAEANKQLADR
                     YLEQAEYEEREKRWADAARSYSRALAGKPSSRLHERVAFCLLAAQGDVKSAVEHARKA
                     VALVPNDPQIRITLARAYLAAGMRESGIAELERAAALAPTDDTLKDYIRRARRGEL"
BASE COUNT         1395 a         2920 c         3011 g         1633 t
ORIGIN      
        1 atcccgccgc tgatgacacc ttcggcgatg aggtgctcgg cttccggcgc gtcgagcgag
       61 cgcagcaggc gcccgtcgct cgccttcacg ccctcgacgt cggtcatgag cacgagcttc
      121 gcggcgcgta gcacgccggc gatgctgccg gcggcggtat cggcgttgac gttcagcgac
      181 ttgccctcgt tgtcgaccgc gatcggcgcg atgacgggga tgaagccgtt cgaaatcagc
      241 tgatcgacga gcccggcctc gaccgattcg acctcgccga cgcggccgag atcgaccgcg
      301 acttcgttgc cgctctggtc tttggccatg accttgtcga gctttttgcc gcgcatgaag
      361 cgatcgtcgc gcccggagag gcccaccgcg cggccgccgt gctggcaaat gagcccgacg
      421 atttcctggt ttacgccgcc acccagcacc atttcgacga cgtccatggt ctcgtcgtcg
      481 gtgacgcgca ggcccgcgtt gaaaacgctc ttgatgccca cgcggtcgag catgcggttg
      541 atctgcgggc cgccgccgtg aacgacgact acgcggatgc cgacgtagcg gagcagacag
      601 atgtccttgg cgaagctctc tttgagaact tcgtcggtca tggcgtgccc gccgtacttc
      661 acgacgacgg tgcgattgtg gaagcgtcgg atgtagggca gcgcctcttc gagcaccgcc
      721 gcttttcgaa tcaggtcgtc catcagggcg gccgccccta tatcacgcgg cggccggagg
      781 ccccagccgc ctggcggaac gcgcaatttt gtcgcaatcg cggcggtttc cggccatcgc
      841 ggcggcgcgc gctcgagcga gccccgagtc ggcgttggtt ccggacggtg acttggtgta
      901 gacccgggct cgatggacgt cctgatggtt gcggccgagc tcgcgcccta cgcgcgagtc
      961 acagcggcgg cggattcgat tgctgctctc gcgaaggctc ttcgccaact gggccacggg
     1021 gtgaccgtcg ccctgccgcg ctcgtcggga tttcagtcga acggcccgct gctcgcgcgc
     1081 cgcttgaccc cgctctccgt cgagccgggg ctcgacgtca ccgtactcga gggccagctg
     1141 ccctccggag tgtcgctcgt gctcttcgac gcgccggagt ttgccgcgga cgagctcacg
     1201 agcgagtcgg ggggtgaacg cgtcgcgctc ctctcgcgcg ctgcacatgc tctcgtgcgc
     1261 gaacgtaagg agcaaggcaa gcgcgtggac gtcgtgcacc tgcacgattt cgcggccgct
     1321 cccgccgccg ctctgctggc gcaagcgccg gagctcgcac cgagcttgct cacgattcat
     1381 tccttcgggc ccgagcgcac cttcgatccg gcgagcctcg cgcgccttgg gcaggttgtg
     1441 gacgagccgc gtgcgcgcat cggcaaacgc gtctcgcccc ttcggcttgg gatggtgaac
     1501 gcgaaagtcg tgaccaccgt gtctcccggc tacgcgacgg agctcggcga cacggctctc
     1561 ggaagcttga ttcaggcctt gccagagccg ctcgccggcg tgctcgacgg ggtcgactac
     1621 gcgacgtaca gccctgcgac ggacgccgcc atcgagagcc gcttcgacgc cgaagatctt
     1681 tcgaacaagg ggcgttgcaa gaccgcgctc gtccgcgcca cgggtttgcg actcgaccct
     1741 gagcggccac tgctcgtcgc gctgctcggg ctttcggatc ccactgggca cgatctgttg
     1801 gtgcgcgccc tgccgcgact tctgaatctt cctctcgcgg tcgtggtctt cggtgccgct
     1861 gacgccgagc gcagcgccca gctcagtgcg ctccaacaac agtatccggg tgatctcggc
     1921 ttgctgctcc gcagtgacga cccggagcta cggcgctgcc tgtctgccgc cgacttcacg
     1981 ctcagcgcgc gctctcattc gcccgctgcg caccaagagc tcttcgcgat gcgctacggt
     2041 gcgctcccaa tcgcgtgcgc gcgtggcgcg gtaaaagacg tcgtcgtgga catcgacgcc
     2101 gagctcgaga ccggcaccgg tttctccttc ggcgagcttt cggaggacga gctcgtcgga
     2161 gccgtgcagc gcgcggtttc ggcatttggc agcccgcgct tcggtcaggt gcggcgccgc
     2221 gcaatgcggc gagatctcgg ttgggatcgc ccggctcggc gctaccttca aatctacaag
     2281 aagctcgctg gcggctgaag cggcgagacg actcgccgta tccagaaggc gtcgagcgcc
     2341 gtgacgagtc cgagcaccag ctggtgcagc gcaaaggccg cgagcacgcg ccagccgccc
     2401 gggcgtccca cgtcgatcca ctcgacgagc cgcgcggcaa cggctacagc gagcattccg
     2461 agcccgaccc gcaaagcgta gccgccgagc aacggcagca cgttggttcg cacgtgggaa
     2521 agcgctgggc ccagcgccgc gccttcgagc tcgggatcga cgaacaagag gccgcgccgg
     2581 acgtcggcga gggcagtcgt tgcaaagagg cccgcgccga gcagtgctgc cgcgggcaac
     2641 aagccgaagc ggcgcgccgg ctcggcgtgg gcggcttgct cgacgaacac gacgatccca
     2701 accagagcga ccttcgcgag cagttccacc gcggcgatcg tgacgagcgg aacgacccgc
     2761 gcgctagccg cgccgaatgc ttttcgcggc cccgacgaag gcgcgaacgt ctgttgaaac
     2821 agcgcgccct gcggcagggt ccgaagcgcg aatgcagcca acacgtacgg ccacatcgtg
     2881 gccgccgcgc cgttcagcaa aggtccgctc actcgcccga gctcagccaa aaagagcccg
     2941 cccggctcga acagcacgcg atcgccgtcg aacaggccgc ccacgccgct gtcggccacg
     3001 gccgcgacga acggcaaggc caagagccag ccgagcaccg agcggcatag ccacaccgcc
     3061 ccgagcgtcc gcgaaaaggc gcggttcatg ggctcacccc tgccaacgcg agagctgcga
     3121 agtaacccag gcgctcgtgg acacgccaga gcacgcggct cgaagtgcaa agttcgttgt
     3181 tgaagagatc gtcgtcgatg agcagcttgt gatcgggatc gacgacgaca cgcgcgagct
     3241 ccgtcgcgcc gcggtattcg aagacgcgga aggcgccgtg gccgtcccag cgctccagct
     3301 ttcgcgtacc gtcccgcgca accaacaaca cgtctaccgg gagctccagc gagccgtggc
     3361 ggtaaacggt ggctcgcccc acgaactcgc gcgtgggttg cgcttgctgc cgggtgatgt
     3421 gctcgcgtcc gctcgcgcgc tcgaagtacc cggccgggtc gctggctctc gtgttttgaa
     3481 ggtcgcgcgc cacgtagtcg acgcgcccgc gatgaaacag cgccgtgtcg aggaaggtcc
     3541 tcgcgtcgga gccgagctcg tcgccgatca cgtcgaggag atcggtggga gtcggatggg
     3601 cgaagcggaa gcgcgcggcg taggctccga gagcgcgctg gaagcgcgct ttgccgtaga
     3661 cgcgctcgag cgtagcgagc agcgtcgcag tgcggctata cacgagcgcg ccgaggtttc
     3721 ggaaactgga gaattcgctc gcggcgcttc ccactggctc gtcctgcgcg cgcgccgctg
     3781 cgaagactcg aaacaggcta tcacccgaga gtgtgattcc gagcgcgccg aagagcgact
     3841 gacgcggtag ctgcgcgccg agcgccgctt gctccgcgta gctgttgagc ccttcgtcca
     3901 agaagggcca gcgcgcctcg ttgctcgcca aaatcccata gaaccattgg tgcccgaact
     3961 cgtgaacgac caccgtttgg acgtcgtgca tgccgaggta cggcgtgtac caatcgccac
     4021 cggtggtgat cagcgtcgga tactccatcc caccggatgc ctctgcgccg ggcggtggat
     4081 gcatgaccgt gagcgtcggg tacggatagc tcccgaacct tcgctcgaag tagggaagcg
     4141 cgaagcgcag ggtgtcctcg ctgagggtcg cggccttctc ttgtcccgcg gggaacagca
     4201 tgcggacgtc gagcccaccc gcggaaaagc gcttttcctg gaatccatcc cacgccgtcc
     4261 acgcgaagtc gtgcaccggc tccgcgacgt agcgaacccg cttgcgcccg ccggccgtgc
     4321 tctggctcac gagcgcgccg gtcgcgccaa ccgtgaatcc ttgcggcacg tccagcgtga
     4381 cgtcgtagcg cccgaagtcg gcgtaaaact cggcttgcgg gtggaagctg aagtgcgaaa
     4441 actctccgtt gtcttcgagg cgggcgagct ttggaaacca ctgggccacg aagtgaaagc
     4501 tccctgagta gccggtgcgc tccatcagct cggggagctg cgactcgaag cgtaaatcca
     4561 gagtgagaga ctcaccgggc tccacggctc gcggtagcgg tacctcgatg tcggtctcgt
     4621 cgtcgacgtc gccggggctg tgacgctgcc ggttcggcca gatatcggcg ttcccgagct
     4681 cgcgcaccgt gagcgacgcg acgtcgatgt agccgaagtc gcgtccgcgc gagtcgtcgc
     4741 gactggcccc gaacggagag cgcagaaata cgctccgctc gttcttgaac gcattcagat
     4801 agagatgaaa aaacagcgag cgcaccgggc tccgcgacgt gttctgccag gcgatggtgc
     4861 cgctgccttt gacgttgtgc ttgcccgcgt cgagctcggc gttcagcgtg tacgacgcaa
     4921 cgagcttcgg agcgcgctcg gctgtcttcg ctgggcgttg ctctggaggg gccccgcgcg
     4981 gccggctcgg cggagcgagc agcgctgcag aaatgccgag cgcggcgagg cccatcgcgg
     5041 cttcgacgat cgctcgtccg cggaagttca cgacgagctc aggcgccgcg cggaaccagc
     5101 gcgatcacgc gctcttctcg cagagtgtag aggatgcgca gcgcatcgag tttcgccatg
     5161 ccgctgatgt cgagcagctc ttcgacgctg gaagtgccgt cgaccaacga cagcaagaag
     5221 cccgaccggt gatcgagcga cagccagcga atctgctcgc tcgggatggc caccgtcacg
     5281 cgctgatcca acgcgccgag ccgcgccgag tacatctgcg tcaacacgtc gcggcaattg
     5341 ttcgcgtagc gttgcgcttc ggggtgctcg ggagcgtttt ccaaaatgcc ctcggcgagc
     5401 acgagcgcgc ccgaaaaatc tccggtcgcg tagcgatcgg caatctttcg gatttgcgga
     5461 tcactcgacg gtgggatgga attcgaagcc tccaccccgg ttattggatc gagcccggtg
     5521 atgtcgagct cgagcgacaa cgcccggcgc gcctcacgcg gcggcaccgc cgccaccagc
     5581 gcgctgtcgt cgatttcgag gacgagctct tcggagaacg actcgtgcgc gggcggcgcg
     5641 gtcgaaagct gcgagttcgg gacctcgctc tcggtcttcg acgtcagcgc cccctcgagc
     5701 tccggtggtg gcggctcgtc gctctgcgtc gcctccatca tttgcttcgc catgagctcc
     5761 ggcggaagct ccggaatcgc ggtcacgcga tcgaaggcgt ggtcgtccag atcgaagcgc
     5821 gggacggtgt cgtcgtccca attgacgtct tccaccggag cctcggcggg ccgcgatggc
     5881 ggaggcggag ctccgcgccg tttgccgtca ccgggagtca ttcggttcat ccgcgggcga
     5941 ctacgccctt gaacatgcgc tgcgtgcgct gcagctgctc gacctgctgc ttgaccgcct
     6001 gcgtgtcctt cgcggtgatg gcgctgcgcg ctttttcgag cgtgctgcgc gccttgttga
     6061 tggcgtcgcg tccgaagtcg gcggtaccga cgacgcgctc gatctcgggg aacatccgct
     6121 cgatttccgc catcagggtt tcggcttctt gcttcgcgcc ctcgaattgt tcgtccgaac
     6181 gacgctcgac caagtagtcc tgcgccgtcg ccatcatgtt cttgatctcg tcttgagtga
     6241 ggccgctcgt cgcggtcacc tggatggatt gggcctggcc cgtctcgaga tccttggcgt
     6301 ggacgctgac gatgccgtcg gtgttgattt cgaaggtgac ttcgatctcg acgtggccct
     6361 tcggcgcgcg ccgaaggccg gtgaggttga attagccgag catctcgttg tcggtggccc
     6421 gcttgctttc gccctgcatc accaggatct tgaccgtcgt ctggtcgtcg cggctcgtcg
     6481 tgaagatctt ggtacggctc gtcgggaccg tcgtattttg agggatcagc tcttcgaagt
     6541 ggctgccgaa agtcatgatg ccgagcgcgt gcggtgtcac gtcgagcagg atcatgtcgt
     6601 ggcggtcttg gaccagggcc gcaccttgaa tcgcggcgcc gactgccacc acttcgtccg
     6661 ggtggacgct cttgttcggc tcgcggccga agaactcctt cacggcggcc tggacggcag
     6721 gcatgcgggt catgccgccg accaagatta cgtcctggat ttcatcgacg tggaggcgcg
     6781 cgtcctcgag cgcctgacgg cagatttcga cggtacgctc gacgaggtcg tccgtcagct
     6841 tctcgagcgt atcgcgcgtc aacgtgcgct ggaggtgaag ggcctcgttg cgaccggtcg
     6901 agatgatgaa cggaaggttg atctccgttt ccttcacgct cgaaagctcg cacttcgcct
     6961 tctcggcggc gtcgcgcagg cgctgcagcg ccatacgatc ttgacgcaaa tcgatgctgt
     7021 gctcttgctg gaagccgttg accaaccaat cgatgatgcg ggcgtcgaaa tcctcgccgc
     7081 cgaggaacgt atcgccggtg gtgctgatga ccttgaagac gccggacgcg ccgatttcga
     7141 ggatgctgac gtcgaaggtg ccgccgccga gatcgtagac cgcgaccgtt cgatcgaaat
     7201 ccttgccgta gccgtacgcg agcgatgccg ccgtcggctc gttgatgata cgaatgacgt
     7261 cgagcccagc gatcgccccg gcgtctttcg tggcctggcg ctggttgtcg ttgaagtacg
     7321 cggggacggt gatgaccgcc ttcttcaccg gctcacccag gtagtcctcc gcgatgatct
     7381 tcatctcttg aaggaccatc gagctgattt cggggaccga ataggtcttg tcgcggagct
     7441 tgatgcgcac gtcgccgtgc ggcccctcga tgatgctgta gctcgaggtg aggatggcgt
     7501 tcttcacctg tggcgagttc cacttgcggc cgatcaggcg cttcgcggcg tagaccgtgt
     7561 tctcggcgtt ggtgatcgct tggcgtttgg cgatgtgacc gacgaggcgt ttacccgctt
     7621 ccgtgaccgc cacgacgctg ggcgtggttt tgtagccgcc ccgattgggg atgacgactg
     7681 gttgatctcc ctcgaggatc gaaacacaag agttggtcgt tccgaggtcg ataccgatga
     7741 cgcgctccat atcgctgctt tcgtgaggct tactgccggg tggtcgtcag agttccccgc
     7801 ggcgcgcgcg gcgaatgtag tctttgagtg tatcatcggt cggcgccagg gctgctgccc
     7861 gttcgagctc cgcgatgccg ctttctcgca tacccgctgc tagatacgcg cgcgccagag
     7921 taatccggat ttgagggtcg ttgggcacga gcgcgacggc cttgcgagca tgctcgacgg
     7981 ccgatttcac gtcgccttga gccgcgagca ggcaaaaagc cacgcgttcg tgcagccggg
     8041 agctcggttt gcctgccaac gcgcgagaat acgagcgggc cgcgtcggcc cagcgctttt
     8101 ctcgctcttc gtattcagct tgctccagat agcggtcggc gagctgcttg ttggcttcgg
     8161 cctgaagatc ctcgaggcgg cggcgcaaga ctggatcatc cggggccagc gaaacggcga
     8221 tcctgagcgc gttgacggct tccaccacct tggcctcgcc catggagctt tcggcccggc
     8281 cgaggtactc gtggacctgg cgccgccggg cgtcgaccat gcgttggtcg tagcggcgct
     8341 tgagatcgtt gacggctcgg tcgcgtgcgc tcggggccgc ggtgccctct tgggacgggg
     8401 gctgaggggg aagggggcta gagcgaactt gtggtgccgg gggaggataa gagaggccca
     8461 gcttgcgagc caaggcgcgg cggcgggcgt ccgcatcgtt gcgcccggcc ggcgcgggca
     8521 cgctcgggcg ggcggcgacc ggttcgctcg gacgtgccac cgcgggatcg ctcggccgca
     8581 cgccggctgg atcgctcggc cgcgccgcaa ccggatcgct tggacgcggc tgagtcggtt
     8641 cgctgggccg cacgttgttc ggatcgctgg taggcgcagg gggagtgctc gatgcgacat
     8701 gcccgggtac gggcgtgttc gcgacccgtt gcgcttctgc ttcgatgcgc gcgcgcgctg
     8761 cgtcgagctc ccgcgcgtaa gcttcctcgt cgttgagctc acgttcgagc gcagcggtac
     8821 gccttcccgc gttcagataa gcgtcgtagt cggctcgcgc gtcgttgcga gtcagggtat
     8881 cgtgggcgtc ggtaacgcgc tgaaacacgc gctcgagctt cgacttgaat cggccgaggt
     8941 tcttcccgta gtaccgatc
//
LOCUS       DASTKS010001219         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1313104, whole genome shotgun sequence.
ACCESSION   DASTKS010001219 DASTKS010000000
VERSION     DASTKS010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313104"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1068)
                     /locus_tag="VFQ35_26035"
     CDS             complement(88..1068)
                     /locus_tag="VFQ35_26035"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9934199.1"
                     /translation="MALTVIVRSGELKTEASITFDAPRIVVGRGDGCEVLLPDPSVSH
                     RHASIRQRGTDYVLIDEGSTNGTFIGPVRLSPQAPRVVKTGDLIRVGRVWLELRVEQV
                     PVTQSQKVVTREIALALVASALSAQGEAKGIRLKVQKGPAAGTELLIENPERAHVLGR
                     GAGVDLPLDDPDLSRRQLELFRRGIPLMVRDLGSKNGSFIDGQRLESGKDVIWPLEKV
                     LAVGQSELVYEDPVRALLQEIEQGEDERMRPDDEVAPPTNVPHVEPPQSEGPPVALSA
                     GGGAPIAEVPRREAAPRRRGSSRVFELLVGLVALAVLGLSLFGLWLLFRS"
     gene            1211..3103
                     /locus_tag="VFQ35_26040"
     CDS             1211..3103
                     /locus_tag="VFQ35_26040"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9934200.1"
                     /translation="MTQPQERRDPEKPRKTVSTLVEVCGNVPGIPVFEAESIDVSPHG
                     MHLRTAYLPEEGAPLVCRFESDGREIVVEGMVAWRREGGRGGEFGVKFTALDSRSVDA
                     LRNLCGMGGEPPKAEVQIGEPGARVRLHIDGLGSPMKARVKAGGSSKVQVGSSLEFLK
                     VGKRLELEDLDIGARRSAEIDGVSVIVDPSTQVPQLVVALRFEGSADSTPSPSVADVA
                     AREPAAGVRIPAGAVTTDAKKSADKPKESASTSASDDEDADDDAPEMRGRLASMAANA
                     EESAKRASETLARAGAGAAQGAASFFKNAGSRLLELRKKSSVPARRTTAPAPGTSAEK
                     PRLRPQSGSSPAIEAIPLRKLSRRTLAIAGGASVATVGILMFALRTPSAPPPGASNGA
                     VNGEVVSEVAPPAKAASPGASAVPNALGTNSDGVVTANVPLFGPTPLATTEPAPLGPP
                     PGSNAAAVEASERADARQSIGAQMNDESFGDESEGEGHAKSSEKSSDKSKSQKPEDVA
                     PWGHGKMHEPTIYRIRLDGPGEKIVGTQEPTGFNVTVPERKVMEAVTAIAKRDDRIAR
                     VRSNNGDAGAQLSFRFKDEVPSYRVRLRRDYVEVLISAPIATSKEKDSKKPEKSEKSD
                     KEKRKK"
BASE COUNT          588 a         1110 c         1081 g          486 t
ORIGIN      
        1 cgatgacctc gagcgctcca tcgagagggc gactcctgaa gacgtcgaac cgagcgcgcc
       61 gctgcaatcg ggtgaagggc agaccgttca ggagcgaaac agtagccaga gcccgaacaa
      121 gctcagcccg agcaccgcca acgcgacgag cccgaccaag agctcgaaga cacgcgagct
      181 gccacgcctg cgaggcgctg cttcacgccg cgggacctcc gcgattggcg ctccgccgcc
      241 agccgacaag gcgacgggcg gaccttcaga ttggggcggc tctacatgag gcacgttcgt
      301 cggcggcgcg acctcgtcgt cgggccgcat gcgttcgtct tccccttgct cgatttcttg
      361 tagcagcgcg cgcacggggt cttcgtagac gagctcgctt tgcccgaccg cgagcacctt
      421 ctcgagcggc cagatcacgt ccttgcccga ctcgagccgc tgaccgtcga tgaagcttcc
      481 attcttggag ccgagatcgc gcaccatcag cgggatgccg cgacggaaca gctccagctg
      541 tcgacgcgac aagtcggggt catcgagcgg caggtccacg ccggcgcctc gcccgagcac
      601 gtgcgcccgc tccgggttct cgatgaggag ctcggttcct gcggcgggtc ctttctgcac
      661 tttgaggcgg atgcccttcg cctcgccttg cgccgagagc gcgctcgcca cgagcgcgag
      721 agcgatctcg cgggtgacca ccttttgcga ctgcgtcacc ggcacctgct cgacgcgaag
      781 ctcgagccaa acgcgcccga cgcggatgag gtcgccggtt ttgacgacgc ggggcgcttg
      841 aggcgagagg cggactgggc cgatgaaggt gccgttcgtg ctcccctcat cgatgaggac
      901 ataatcggtg ccgcgctggc ggatcgaggc gtggcgatgg ctcacgctgg ggtccggcag
      961 caagacctcg cagccgtcgc cccggccgac gacgatacgt ggggcgtcga acgtgatgct
     1021 cgcttctgtc ttcagctcgc cagagcgcac gatcacggtc agcgccattc gggacgccaa
     1081 tcatgatggc gtgccgccac tcggcaaagg gaaagcgccg cgcgaacgga aacttggccc
     1141 tctgcctcga tgcgccttag gtcgaggtgg gtacgggccg ctcgcgagcc gtccggaccg
     1201 gaggatccca atgacgcagc cgcaagaacg ccgtgacccc gaaaagccgc gcaaaaccgt
     1261 gtccacgctc gtggaggtat gcggcaacgt ccccggtata ccggtcttcg aagccgagtc
     1321 catcgacgtg agcccacacg gcatgcacct tcgcaccgcc tatttgccgg aagagggcgc
     1381 gccgctcgtt tgccgcttcg agagcgacgg ccgcgagatc gtggtcgagg gaatggtcgc
     1441 ctggcgccgc gaaggtggtc gcggcggcga gttcggggtg aagttcaccg cgctcgacag
     1501 tcgcagcgtg gacgctctac gcaacctttg cgggatgggc ggcgagccac cgaaggccga
     1561 ggtgcagatt ggtgaacccg gcgcgcgcgt gcgccttcac atcgacggcc tcggctctcc
     1621 gatgaaggcc cgcgtgaagg cgggcggctc cagcaaggtg caggtgggct cgagcctcga
     1681 gttcttgaag gttggcaagc gactcgagct cgaagatttg gacatcgggg cccgccgatc
     1741 ggccgagatc gacggcgtga gcgtcatcgt ggacccgagc actcaggtgc cccagctcgt
     1801 cgttgcgctg cgcttcgagg ggagcgccga ctcgactccg tccccgagcg tcgcagacgt
     1861 cgccgcgcgt gaacccgctg ccggtgtccg tatccccgcg ggtgccgtca ccaccgacgc
     1921 caagaagtcc gccgacaagc ccaaggagtc cgcctcgacg agcgcttcgg acgacgagga
     1981 cgccgacgac gacgcaccag aaatgcgcgg tcgcctcgct tcgatggcgg cgaacgcaga
     2041 ggagagcgcc aagcgcgcga gtgaaaccct ggcgcgcgct ggcgccggcg ccgctcaagg
     2101 cgcagcgagc ttcttcaaga acgccggcag tcgcttgctc gagcttcgca aaaagtcctc
     2161 cgtgcctgcg cgtcgcacga ccgctcccgc gccgggtacc tccgcggaga aacccaggct
     2221 acgtccgcaa agcggcagct ctccggccat cgaggcgatc ccactgcgca agctgtcgcg
     2281 tcgcaccctc gcgatcgccg gtggagcttc ggtcgcgacg gtgggcattc tgatgttcgc
     2341 gctgcgcacg ccgagcgcgc cgcctcccgg cgcatccaac ggcgccgtga acggcgaggt
     2401 cgtgagcgaa gtcgcacctc cggcaaaggc tgcctcgccc ggtgcttcgg ccgttccgaa
     2461 cgcgctcggc acgaactcgg acggtgtcgt caccgccaac gtcccgctct ttggtccaac
     2521 tccactcgcc acgaccgagc ccgcgcccct cgggccaccg ccgggcagca acgccgccgc
     2581 ggtggaagct tccgagcgag ccgacgcgcg gcagagcatc ggcgcgcaga tgaacgacga
     2641 gtcgttcggc gacgagtcgg aaggcgaagg tcacgcgaag agctccgaaa agtcctccga
     2701 caagagcaag tcgcagaagc ccgaggacgt cgcgccgtgg ggtcacggca aaatgcacga
     2761 gccgaccatc tatcgcattc gcctcgacgg cccgggcgag aaaatcgtcg gtacgcagga
     2821 gcctactggc ttcaacgtga ccgtccccga gcgcaaagtg atggaagcgg tgaccgccat
     2881 cgccaaacgc gacgaccgca tcgcgcgcgt gcgctcgaac aacggcgacg ccggagcgca
     2941 gctctcgttc cgcttcaagg acgaagtgcc gagctatcgc gtacgcctgc gcagagacta
     3001 cgtcgaggtg ctgatctctg cgcccatcgc gacgagcaaa gaaaaggact ctaagaaacc
     3061 ggagaagagc gaaaaatccg ataaggagaa gcgcaagaag tgagcgttcg ctcgggagag
     3121 cctccgctcg ctgaggcttc ggctctccca cctcctgcgc gctttccctg ctcaccgcgt
     3181 gcgcttcgcg ctgcgccctg gtgaaacggc gatatacttc ggggttgaat gacgaaacgg
     3241 cgcacgtttt ctcccccgga gccat
//
LOCUS       DASTKS010001220         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1984078, whole genome shotgun sequence.
ACCESSION   DASTKS010001220 DASTKS010000000
VERSION     DASTKS010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984078"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFQ35_26045"
     CDS             complement(<1..385)
                     /locus_tag="VFQ35_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934201.1"
                     /translation="MIVCGVRLYWAVRVVSSLSAVAVACTAPRNAAWPDGDAGALPFQ
                     FEAEPNELASGVAIDVIEAVESNAVAGASELSFVAGDSVVQYSASGELRRIPLYGGLP
                     TVIASGVAKGSLVTGDARGTYWVTPD"
     gene            485..1120
                     /locus_tag="VFQ35_26050"
     CDS             485..1120
                     /locus_tag="VFQ35_26050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2621 family protein"
                     /protein_id="HET9934202.1"
                     /translation="MFHGLSHVDVSVTDLERALKLYRDGLGFPVTSRGEGWVELNAAT
                     AAIRLIESPSVERRASLRIEAVSVERGADQLVAAGAAVVYPATRTPLMTLEACLHDAD
                     RNAITLWRALTEDEYGFDPELPSQQRWDADAEALLKSLLHSVPALFRGLARRKVVKEA
                     ESRAGSVGRVTRDLTIRSFISAQSPPNRRRLLEPLRARGIDPDAYKDEFEA"
     gene            complement(1174..3270)
                     /locus_tag="VFQ35_26055"
     CDS             complement(1174..3270)
                     /locus_tag="VFQ35_26055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934203.1"
                     /translation="MKALLFVSALLLLSTTMTATVSAQGAPPCEFAVSGDVKKLYSGA
                     LRERLELVGTDACKLALVQAGPVRLVNGSSELRATAHTHENGANVVVLETDEIPTNLV
                     LGDHDWEIQSDVPHKTVLGKVRLEAAGPAATVAADSNGNPVLEVSYGRLDLDVSHSLE
                     SSDGQAVVTNLKGEEIGYIRNEVQIQRPAELASRARDIAQRRQQPALSRCQELWAEAN
                     CVWKNAAQQAITIEKCELAEWKCSPTANATRASLESCLASKLEERVSVCEKEIERGDN
                     VLSDADGSRLEKWRDAVSPWRLIPSSWRADVWLYEPAWWYDTQARQSSLAGIRFAVMA
                     PHTEPLLAHLELRDGDKLLLRTQLVLAKGARLESLPLPIASALDVRCAGKALSREALT
                     SPLQKTPALDIIGYNETRAVDDADVEEGRCFVVYHSAKVLEPQLAARGSKKSRYSERT
                     KRALLATYGQQELEITISRGGAAQKSTTRWLDPAQDDVIPVPALKEGSGVYSVSVRLR
                     GPSTPIVQYRRGPRQTASSTAASADLGELEFRASLRPRGHLGWRRAQIRTFVTFPLRF
                     TGMRFPAKASELRTSNDSTTVQVVPVKAGIMAALEPWNYDTDRNPLTPPMRVMTGFHL
                     YDLSNGDFAPSFLLGGSLTFPILQTAATGGTGNLGTDVAIGAFWEVDLQERHPIKDGS
                     HFLLTLGVDVLTLFAK"
     gene            complement(3267..>3869)
                     /locus_tag="VFQ35_26060"
     CDS             complement(3267..>3869)
                     /locus_tag="VFQ35_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934204.1"
                     /translation="RVAPCVTLAFTALLTHCESDTGFACGTQLRKDSTIIQTCTRNLE
                     ICVCATTSCAKPDLGLEDDSAGAAHASSKCESGYRYVEAPFADPGWAGRCVPKSHLRE
                     YPYVPNASNAGPLCPTLPYPVKGEGGTSSGGAASGGTGGMSSSGSDSGGTNASGNGAG
                     GGSSGGVAFGGSSQTLGGSANAGESTIGGGGTGLTAGVEG"
BASE COUNT          650 a         1270 c         1231 g          718 t
ORIGIN      
        1 agtccggagt cacccagtaa gttccacgcg cgtcgcccgt cacgagcgag cccttcgcaa
       61 cgccggatgc aatcaccgtc ggcagccccc catagagcgg aatccgcctc agctcgccgc
      121 tagcggaata ttggacgact gaatcccccg cgacgaagct gagctcgctt gctcccgcca
      181 cagcgttcga ttccactgcc tcgatcacgt cgatggcgac gccgctcgcg agctcgttcg
      241 gttcggcctc gaattggaac gggagagcgc ccgcgtcccc atctggccaa gcggcattcc
      301 gcggcgcggt gcacgcgacg gcgaccgcgc tcaaactcga cacgacgcga actgcccaat
      361 agagtcgcac gccacagact atcaccagaa cgggcacgtg agagcccgca ccgggctctg
      421 gctgggtaga aatgtgcgga agcactggtg gttggcggcg cgttgtcgct attcttcccc
      481 ggtcatgttt cacggcctca gtcatgtcga cgtatcggtg accgatctcg agcgtgcatt
      541 gaagctctac cgcgacgggt tgggctttcc tgtcacctct cggggagagg gttgggtgga
      601 gttgaacgcg gcgaccgcag cgatccgcct gatcgagagt ccctccgtgg agcggcgagc
      661 ctcacttcga atcgaggcgg tctcggtcga acgaggtgcg gaccaactgg tggcggcggg
      721 cgcggcggtc gtttatcccg cgacgcgcac gccgttgatg acgctcgagg cgtgtctgca
      781 cgatgccgac cgcaacgcga tcactttgtg gcgcgcgctc accgaagacg agtacggctt
      841 cgatccagaa ctcccatcgc aacagcgctg ggacgcggac gcggaggcgc tgctcaagtc
      901 gttactccat tccgttcccg ccctatttcg agggctggct cggcgcaagg tggtgaaaga
      961 ggcggagtct cgcgccggtt cggtcgggcg tgtcacccgt gatctgacca ttcgttcgtt
     1021 catcagcgcc cagtcaccgc ccaaccggcg gcgcttgctc gagccgctga gagcgcgcgg
     1081 aatcgatcca gacgcctaca aagacgaatt cgaagcgtga ccgcgacgga ggtcccctcc
     1141 gacgagcgcc tttttgcgct atcgggcagc gctctacttc gcgaagagcg tgagaacgtc
     1201 cacaccgagc gttagtagga agtgacttcc gtccttgatc ggatgacgct cttgcaagtc
     1261 gacctcccag aacgcaccaa tagcgacgtc ggttccaaga ttgccggtgc ccccggtggc
     1321 cgcagtctgc aagattggaa aggtgagcga acccccgagc aagaacgagg gcgcgaagtc
     1381 gccgttcgag aggtcgtaga ggtggaaacc ggtcatcacg cgcatcggag gcgtgagggg
     1441 gttgcgatcc gtgtcgtagt tccagggctc cagggccgcc atgatgccgg cctttacggg
     1501 gacgacctgt acggtcgtac tatcattgct ggtgcggagc tcgctcgcct tggcggggaa
     1561 gcgcatgccc gtaaatcgca ggggaaaggt gacgaaggtg cgaatctgag cgcgtcgcca
     1621 gccgaggtgc cctcgcggtc gcaggctggc gcgaaattcg agctccccca agtctgcgct
     1681 cgcggcggtg ctcgacgcgg tctgacgcgg tccgcgccga tattggacga tcggcgtcga
     1741 aggccctctg agccgcacgc tgaccgaata cactcccgat ccctctttca acgccggaac
     1801 cggaatgacg tcgtcttgcg cgggatctag ccatcgggtc gtggacttct gcgcagcgcc
     1861 gccgcgcgag atcgtgattt cgagttcctg ctgcccatat gtcgcgagga gggctcgttt
     1921 cgttcgctcg gagtagcggc tcttcttcga gcccctcgcc gcgagttgcg gctccagcac
     1981 tttcgccgag tgatacacta cgaaacagcg accttcttcc acgtcggcat cgtccacggc
     2041 tcgcgtttcg ttgtagccaa tgatgtcgag agccggggtc ttctggaggg gactcgtaag
     2101 cgcctccctg gagagcgcct ttccggcgca tcggacgtcg agcgcgctcg cgatggggag
     2161 tggcaggctc tcgagccgcg cgcctttggc gagcacgagc tgcgttcgca gcaggagctt
     2221 gtcgccgtcg cggagctcga ggtgcgcgag gagcggctcg gtgtggggag ccatgaccgc
     2281 gaatcgaatc ccagccaagg agctctgccg cgcttgtgtg tcgtaccacc acgccggctc
     2341 gtacagccaa acgtcggcgc gccacgagct cggaatcagt cgccacggtg agaccgcgtc
     2401 tcgccacttc tcgaggcggc tcccgtcggc gtcgctgagg acgttatcac cgcgttcgat
     2461 ctctttttcg cagacgctca ctcgctcttc gagcttgctc gccaaacacg actcgagact
     2521 ggctcgggtc gcgttcgcgg tgggagaaca tttccactcg gccagctcgc atttctcgat
     2581 ggtgatggct tgttgcgcgg cgttcttcca gacacagttg gcctccgccc agagctcttg
     2641 acaccggctc agcgctggct gctggcgacg ttgcgcgatg tcgcgcgctc gggacgcgag
     2701 ttctgctggt cgctgaatct gaacttcgtt ccgaatgtat ccgatttcct cgcccttcag
     2761 attggtgacg acggcttgcc catcactcga ctcgagtgag tgcgaaacgt cgagatcgag
     2821 ccgaccgtag ctcacctcga gcacggggtt gccgttcgaa tcggccgcga cggtcgcggc
     2881 cgggcccgcg gcttcgagcc gaaccttgcc gagcaccgtc ttgtgcggga cgtcactctg
     2941 aatttcccag tcgtgatcgc ccaggaccag attcgtcggg atctcgtccg tctctagcac
     3001 gacgacgttc gccccgtttt cgtgcgtgtg cgcagtggcc ctgagctccg agctgccatt
     3061 caccaagcga actggccccg cctgaaccag cgcgagtttg cacgcatccg tgcccacgag
     3121 ctcgagccgc tctcgcagcg cgcccgagta gagcttcttc acgtccccgc tcaccgcgaa
     3181 ctcgcacggg ggagcgccct gagcggacac ggtcgcggtc atggtcgtgg acaggagcaa
     3241 cagcgccgaa acgaatagaa gagccttcat ccttcaactc ccgccgtcag accggtgccc
     3301 ccaccgccaa tcgtggactc gccggcgttg gcgctgccgc ccagcgtttg gctcgagcct
     3361 ccgaacgcga cgccacccga gctcccgccc ccggccccgt tgccgctcgc gttggtcccg
     3421 ccggagtcgc taccactcga gctcatgccg ccagtgccgc ccgacgcggc gccgcccgag
     3481 ctcgttcccc cctcgccttt gactgggtag ggcagcgttg ggcagagcgg ccccgcgttg
     3541 ctcgcgttgg gtacgtaggg gtactcacgc aggtgacttt tcggcacgca acgaccggcc
     3601 cagccaggat ccgcgaaggg agcctcgacg taacgatagc cgctctcgca cttggacgaa
     3661 gcatgagccg cgccggcgct gtcgtcttcg agcccaaggt ctggctttgc gcacgaagtc
     3721 gtagcgcaca cgcagatttc gaggttgcgc gtgcaggtct gaattatggt gctgtccttg
     3781 cggagctggg taccacacgc aaaccctgta tccgactcac agtgggttag aagagcggtg
     3841 aacgccagcg tgacgcaagg agccacgcg
//
LOCUS       DASTKS010001221         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1313539, whole genome shotgun sequence.
ACCESSION   DASTKS010001221 DASTKS010000000
VERSION     DASTKS010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313539"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFQ35_26065"
     CDS             complement(<1..468)
                     /locus_tag="VFQ35_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934205.1"
                     /translation="MDQFSAHLDRGWDLVQRGDPRGAEVSARRALEIDSQSPEAYNLL
                     GYVAALKGEYDEAIDHYRQAIALDDTYLEAMLNAAEVYIHPLGEFDEAISMCDQALDL
                     AETDDETVDALLLKFDALLGKGEIDEAAEVCARFPHGPFENPSHVFLVGRAYYE"
     gene            complement(663..2435)
                     /locus_tag="VFQ35_26070"
     CDS             complement(663..2435)
                     /locus_tag="VFQ35_26070"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9934206.1"
                     /translation="MTVRSLRAPVGLVVGAFALSGCERAAPPAPAPARSVSVTTQAGR
                     PELPRELIGTARAAEEPHAAGSAAPPLEAPPVAAPPEVPTAWSVAPSLPADYKGPLFV
                     VTQLAAGVYVDASFDSKKIGYLRNGSKVPVSGEATSKKNCTGGWYHLNSGGYVCGNLG
                     TTDLSKPEVKFAGGAPNLGDILPYPYARNAKNGTPLYHSVPSREQMERYEPYLLEKKD
                     SESSEKSDKRIALQKASSAVAAGGTSGTSVATTTPSATELAAAIADGGILPPPGTGAD
                     PTPDKPWWQREDTKDRLHELKLQELESDADDVLAKRMVAGFYVAVDKTFRWNNRSWYK
                     TTKGLVAPSDRFWQASGPKFKGVELDGATWKLPVAWVYGGRKSVGTYEIDPSAKGAKN
                     AKSYERFEAMPLTGKTIEHGGSRYEELADGTWVKQSQVRIARMPSLPAGLGPNERWID
                     VNLKTQTLVALVGDRPIYATLISSGKESKIKEKDHATPTGEWRVREKHITTTMDGDGT
                     AAGDLPYSIEDVPYVMYFNRSYALHGAFWHNNFGVQMSHGCVNLAPLDAKYLFFFADP
                     PIPVGFHGVWSNDAHPGTRVVIHE"
     gene            2568..>2807
                     /locus_tag="VFQ35_26075"
     CDS             2568..>2807
                     /locus_tag="VFQ35_26075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934207.1"
                     /translation="MDVQTLQRGPRTTRQRIWTLCLVVSLVLHLPFTPFAGLFALLGF
                     LQPNEVDEGPPDPALTEIPIDVATDLGTAESAPAPP"
BASE COUNT          443 a          890 c          928 g          546 t
ORIGIN      
        1 ttcgtagtac gcgcgcccaa ccaagaacac gtggctcggg ttctcgaagg ggccgtgcgg
       61 gaagcgcgcg cacacctctg ccgcctcgtc gatctcgcct ttgccgagca gcgcgtcgaa
      121 cttgagaagc agcgcgtcga ccgtttcgtc gtcggtttcc gccaaatcca gcgcctgatc
      181 acacatgctg atggcttcgt cgaactcacc gagcggatga atgtacacct cggccgcgtt
      241 cagcatcgcc tcgaggtagg tgtcgtcgag cgcgatcgct tggcggtaat ggtcgatggc
      301 ctcgtcgtac tcgcccttca gcgcagcgac gtatccgagg aggttgtagg cctcgggcga
      361 ctggctgtcg atttcgagtg ctcgtcgcgc agagacctcg gcgccgcgcg ggtcgccgcg
      421 ctgcaccaga tcccaaccgc gatcgaggtg agccgaaaat tgatccatgg gagcccgact
      481 tgatggtccg gaggcgcgtc tccgtcaact ccatccggcc cacatctgcg tcgcttaagg
      541 ccccgcgcgc caacagcgtt ttccgagctc ggccgagtgg ccgctgagaa ccgggcgttt
      601 cggagtgagc cgccggtcgt cgcaccccgt ggggtaacag cgggcaacgg ctcagttcga
      661 gttcactcgt ggatcacgac gcgggttccc ggatgagcgt cgttggacca aacgccgtgg
      721 aaaccgacgg gtatcggagg gtcggcgaaa aagaacaggt acttcgcatc gagcggcgcg
      781 aggttcacgc agccgtggct catctggacg ccgaagttgt tgtgccaaaa ggcgccgtga
      841 agagcgtagg agcggttgaa gtacatgacg tacggcacat cttcgatgct gtaggggagg
      901 tcgcccgcgg ccgttccgtc gccgtccatc gtggtggtga tgtgcttttc gcggacgcgc
      961 cactcgccgg ttggcgtcgc gtgatccttt tccttgatct tcgattcctt gccgctcgag
     1021 atgagcgtcg cataaatcgg gcgatcgccg acgagcgcca ccaaggtttg cgtcttcagg
     1081 ttgacgtcga tccaacgctc gttcgggccg agcccagccg gcagtgacgg catccgcgcg
     1141 atgcggacct gcgactgctt cacccacgtt ccatcggcga gctcttcgta gcggctgcca
     1201 ccgtgctcga tggtcttgcc ggtgagcggc atcgcctcga agcgctcgta gctcttcgcg
     1261 ttcttcgcgc ccttcgcgct cgggtcgatc tcgtaagtgc cgacgctctt tcgtccgcca
     1321 tagacccacg cgactggcag cttccacgtc gcgccgtcga gctcgacacc tttgaacttc
     1381 gggccactcg cttgccaaaa gcgatcgctc ggcgcgacga gccccttggt ggttttgtac
     1441 cagctgcgat tgttccagcg gaacgtcttg tcgacggcga cgtagaagcc ggcgaccatg
     1501 cgcttggcga gcacgtcgtc ggcatcggac tcgagctctt gcagcttgag ctcgtgcagg
     1561 cgatctttcg tgtcttcgcg ctgccaccaa ggcttgtccg gagtcggatc tgcgccggtg
     1621 ccgggcggcg gtaggatccc gccgtcggcg atggcagccg cgagctccgt cgcgctcggg
     1681 gtcgtggtcg cgaccgaagt acccgaggtg ccaccggcgg cgacggcgct actcgccttc
     1741 tggagagcga tgcgcttgtc gctcttctcg ctcgactcgg agtccttctt ttcgagcaga
     1801 tatggctcgt agcgctccat ctgctcgcgc gaagggacgc tatgatagag cggcgtgccg
     1861 ttcttcgcgt tgcgagcgta cggatagggc aaaatgtcgc cgaggttcgg cgcgccgccc
     1921 gcgaacttca cctccggctt cgagagatcg gtcgtgccga gattgccgca cacgtaaccg
     1981 ccggagttga ggtgatacca gccgccggtg cagttcttct tggacgtggc ttccccggaa
     2041 acgggaactt tactcccatt tctcaggtaa ccgatcttct tcgaatcgaa gctcgcgtcg
     2101 acgtagacgc ccgccgcgag ctgcgtcacg acgaaaagcg ggcccttgta gtcggcgggc
     2161 agggaaggcg cgacggacca agccgtgggg acctcgggcg gagcggcgac gggaggcgct
     2221 tcgagcgggg gagccgcgct accggcggcg tgtggctctt ctgcagcgcg ggcggtgccg
     2281 atgagctcac gaggcaattc ggggcgaccc gcttgcgtcg tgacgctcac gctgcgtgcc
     2341 ggggccggag caggaggcgc cgcgcgctca caaccggaga gagcgaaggc tccgaccacg
     2401 aggccgacag gcgcacgaag cgaacgaacg gtcattctga ggtctctttc cacagtgggg
     2461 cggttctggc cacttcttgg gcttgcttgg cgcgtttgga agcggcgtgc cacccctcgc
     2521 agagagcgaa ttcgcgcggt agaaggctcg gtactatctt gccgggtgtg gatgtccaga
     2581 cgcttcagcg tgggccgcgc accacgcgtc aaaggatctg gacgctgtgc ttggtcgtgt
     2641 cgctggtttt gcacctgccg ttcacgccgt tcgccggtct gttcgcgttg ctcggcttct
     2701 tgcagccgaa cgaagtcgac gagggacctc cggatccagc gctgaccgag atccccatcg
     2761 acgtcgccac cgacctcggt acggcagaat cggcgcccgc tccgccc
//
LOCUS       DASTKS010001222         5317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1314003, whole genome shotgun sequence.
ACCESSION   DASTKS010001222 DASTKS010000000
VERSION     DASTKS010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314003"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFQ35_26080"
     CDS             complement(<1..557)
                     /locus_tag="VFQ35_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934208.1"
                     /translation="MGNRFGKAYLRLSLLGLSLAGCTVSTASSDEATDSDAWADAGTV
                     VTNDQTSARQAGEGSESLDRVLDPHERSRMSDWLAQNRIANAIVQKRLHTSDGHKVDC
                     VAIRDQPSMRGGSMTRVATPPKLATESDAPVNYAPSVSTLFDANDAASGCDAGTVPIL
                     EVSQQTLTRFRTLEDFFQKVPSHLSG"
     gene            complement(936..2042)
                     /locus_tag="VFQ35_26085"
     CDS             complement(936..2042)
                     /locus_tag="VFQ35_26085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934209.1"
                     /translation="MPLRRHLTSALVASIVACVAGCGGSQDHGASLIAPDSMGGNAAL
                     GGRSSGKGGSTAAGDASGGAADSSGGALNAGASNGGAPSAGAANGGAPNAGSANAEGG
                     AVNANGGTAGGGGEVALSLCNPGANWTLMTRIPSASTPSFARFGGISADELTIAWTAA
                     NGDAFVATRTAMTAEFGAPSKVNSSPLAVGRVALSPTGAYLIGVSADQKNLVAFALKG
                     DAWSMTDGPEFGMLRMSSEGDSTLSDPVLGSDKRSLYFLRTVGGVSSIYASTWDPSAR
                     IWGAPSSLGNPELTASAGHAMRPTGASRDGRTLFFFDEASRTERAAFRPDAEAAFDVF
                     TTLGGFVGATANLGCTTLYYEDRDDSGVGVFVAR"
     gene            complement(2047..4746)
                     /locus_tag="VFQ35_26090"
     CDS             complement(2047..4746)
                     /locus_tag="VFQ35_26090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934210.1"
                     /translation="MDSSSAQSTRWSTLARRFAVLLLLLVAACSKKDRSALVAGGVDV
                     GEGSLPSTDSCATPGHKGCACSDAGAIVECGDVVRMSGDYVTCSMGYARCDGKTWGAC
                     EGNRVVAQSLPNRALGSNGYQLLSTTGGCANPCDPVCTAVTGTPTDLPSGKPLTVTDA
                     GVSITPTTGTSGGTGPCKGLWCNVVSCGSKPATSISGIVYDPAGLNPLYNAYVYIPVD
                     PTAALPAFTSGATCDTCAGTGNFAAVSVAQTGADGKFTLKNVPAGSNIPIVVQMGKWR
                     RKTTIPSVTSCQDNAVGSTFTRLPRNRFDGDGNTADIPKMAIASGSADPFECLLLKAG
                     IDASEIQLPSAGARIDYYVYNGVDRAPGGAPAGDTLTGNLTTLKQYDVVLLPCEGKEI
                     SARSTNAPNLVSYTSAGGRVFTTHFGYSWLATPLSGVARNQTQFYGTADWSKLNQSDY
                     NDPTTGTIDQSFPKGQAFAAWLKNVGASTTLGQITLNEPRHDAIKALNPPSQSWVTGF
                     SKLNTTKTPDMLLSMTFNMPTNVAADKQCGRVVYSDFHVSADALVSSSSTCTANTDCG
                     YGATCNAPVVGKCSTETCYGSSDCSDSSYSCNGGTYGTCVASGSCTSDSQCSSDMKCS
                     GSPKKCVPRSCSRNSDCGWWGSCKSGTCQASACGTNSDCGSAQSCSGAAVGTCKKTCT
                     KDSDCASWWYNTTCVSGQCQGCLTANDCPGSSTTCVGGSAGGCSSTSSYFPLTCRNGT
                     LSAQEKALEFMLFDLTACVSPDSWTPPAPSTVYNPVTFTLDFSATCSGVLKPVWRELD
                     WQASIPTGASIDFSGQTASTVAGLASAQSVKLGSATTSTALPAWDVAVIETSTDGAFR
                     TANPRIASASQFRLTVTLNPTSDQKASPTLSQWKVQYDCVDTE"
BASE COUNT          938 a         1717 c         1712 g          950 t
ORIGIN      
        1 ccggaaagat gagacggcac cttctggaag aagtcttcca aggtacgaaa gcgcgtcaac
       61 gtctgctggc tgacttcgag gatgggaacc gtgccggcgt cgcagccaga agccgcgtcg
      121 ttcgcgtcga acagcgtact cacggaaggg gcgtagttca ccggggcatc gctctccgtc
      181 gctagttttg gcggcgttgc gactcgagtc atgctgcctc cgcgcatcga cggttggtct
      241 cgaatggcta cgcaatcgac cttgtgccca tcgctggtat gaaggcgctt ttgaacgatg
      301 gcgttggcga ttcggttctg ggcgagccaa tcgctcatgc gtgaacgctc gtgcgggtcc
      361 aggactcggt cgagcgactc cgaaccttca ccggcttggc gggcactcgt ttggtcgttc
      421 gtcacgacgg tgccagcgtc cgcccaagca tcggagtcgg tggcctcatc gctgcttgcg
      481 gtgctcacgg tgcaacccgc cagcgatagg ccgagcaacg aaaggcgcaa gtatgccttg
      541 ccaaagcgat ttcccatcca accactcgct tggatcacgt cccacaattt gttagcggca
      601 ggcgcgactc tgcttgcgcg cgacgtctac gtcaatggtc atgagttgtg tagatcgagc
      661 ggcgcaattc gccgactcga acggcgcgct ctctattcga gcttcaaatg gctggttctc
      721 tcacgcgcgt gtgaagtcgt tcaccgactc gcgggcgcgc tgctgcaatc gcctgtaatg
      781 ccaacggacg accgcgcgca agcgctgaca cagcgagccc gcggagggcc cagttcacgt
      841 cgcgtgacgg cgctaagggc tccgcgctgg ccctcgagac aggcgcgctc aatccgagac
      901 gaactcgctc gatccgagat cgactggcct gcgagtcagc gagcgacgaa gacgccgacg
      961 ccggagtcgt cgcgatcctc gtaatagagc gtcgtgcacc cgaggttcgc cgtcgcgccg
     1021 acgaaccccc caagcgtcgt gaagacgtcg aaagccgcct cggcatcggg ccgaaacgcg
     1081 gcgcgctccg tcctcgacgc ttcgtcgaag aagaagagag tgcggccgtc gcgagaagcg
     1141 ccggtcgggc gcatggcgtg ccctgcgctc gccgtgagct cggggttgcc aaggctcgaa
     1201 ggagcccccc aaatgcgtgc actcggatcc caggtcgacg cgtaaatgga gctcacgccg
     1261 cccaccgttc gcaagaagta gagcgagcgc ttgtccgagc cgagcacggg gtcggacagg
     1321 gtcgagtcac cctccgaaga catccggagc atgccgaact cggggccatc cgtcatgctc
     1381 caggcgtcac ctttgagcgc aaacgcgacg aggttcttct gatctgcgct caccccgatg
     1441 aggtaggcgc cagtcgggga tagcgccacg cgacctaccg cgaggggact cgagttcacc
     1501 ttgctcggag cgccgaactc ggcggtcatt gccgtgcgcg tcgcgacgaa cgcgtcgccg
     1561 ttcgccgcgg tccaggcgat ggtgagctcg tcagcactga tgccaccgaa gcgtgcgaag
     1621 ctcggcgtgc tcgcgctcgg gatccgggtc atgagagtcc agtttgcgcc ggggttacac
     1681 aatgacagcg caacctcacc accgccgcct gccgttccgc cgttcgcatt cacggcaccg
     1741 ccttcggcgt ttgcactgcc agcgttgggc gcaccaccat tcgcagcacc tgcgctcggg
     1801 gcaccgccgt tcgaggcgcc ggcattcaga gccccgccac tcgagtctgc cgctccgccc
     1861 gaggcatccc ccgccgcggt gcttccgcct ttacccgagc tcctacctcc gagagctgcg
     1921 tttccaccca tcgaatccgg cgcgatgagt gaagcgccgt gatcctggga gccaccacaa
     1981 ccagccacgc aagcgacgat gctcgcgacg agcgcgctgg tcagatgccg tcggagcggc
     2041 acgtgcctac tccgtatcaa cgcagtcata ttggaccttc cactgagaca aggtaggaga
     2101 tgctttctga tccgaagtcg ggttgagagt tacggtgagc cggaactggc tcgccgaggc
     2161 gatgcgtggg ttcgcggtcc ggaaggcgcc atcggtcgac gtctcaatca ccgccacgtc
     2221 ccaagccggc agcgccgtcg aggtggtcgc gctaccgagc ttcaccgatt gagccgacgc
     2281 caagcccgcc acggtgctcg ccgtttgccc cgagaagtcg atggatgcac cggttgggat
     2341 cgaagcttgc caatcgagct cgcgccacac gggcttcaga acgcccgagc aagtggccga
     2401 gaaatcgagc gtaaacgtca ccggattgta gacggtggag ggagcaggcg gcgtccagct
     2461 gtccggcgac acgcaagccg tgagatcgaa cagcatgaac tcgagcgctt tttcttgcgc
     2521 gctgagcgtc ccgttccgac acgtgagcgg gaagtagctg gaggtcgacg aacacccgcc
     2581 cgcggagccg cccacgcacg tcgtggacga accagggcag tcgttcgcgg tgaggcagcc
     2641 ctggcactgg ccagaaacgc acgtggtgtt gtaccaccag ctcgcgcagt cgctgtcctt
     2701 ggtgcacgtc ttcttgcagg tgccgacggc tgctccggag caactttgcg cgctgccgca
     2761 gtcggagttc gtgccgcacg ccgaggcctg acaggtgccg gatttgcagc ttccccacca
     2821 gccgcagtca gagttcctgc tgcaggagcg aggcacgcac ttcttcggcg agccggagca
     2881 cttcatgtcg ctcgagcatt gggagtcgct cgtgcaactt cccgaggcca cgcacgtccc
     2941 ataggtgccg ccgttgcacg agtaactgct gtcactgcag tcggagctcc cgtagcaagt
     3001 ttcggtcgag cactttccga ccaccggcgc gttgcacgtc gctccgtagc cacagtcggt
     3061 gtttgcggtg caagtgctgc tggagctcac cagggcgtcg gccgaaacgt ggaagtcgga
     3121 gtacacgacg cggccacatt gcttgtccgc ggcgacgttg gtcggcatgt tgaacgtcat
     3181 gctgagcaac atgtcgggcg tcttcgtggt gttgagcttc gagaagcccg taacccacga
     3241 ttgcgacggc gggttcaacg ccttgatggc gtcgtggcgc ggctcgttga gcgtgatctg
     3301 accgagcgtc gtggacgcac cgacgttctt gagccaggcc gcgaaggctt gccccttcgg
     3361 gaacgactga tcgatggtgc cggtcgtcgg gtcgttgtag tcggactggt tcagcttgga
     3421 ccaatcggcg gttccgtaga actgcgtctg gttgcgcgcg acgccgctga gcggggtggc
     3481 gagccacgag tagccgaagt gcgtcgtgaa gacgcggcca ccggcgctcg tgtacgaaac
     3541 caggttcggc gcgttcgtcg accgcgcgga gatttctttg ccttcgcacg ggagcaagac
     3601 gacgtcgtac tgcttcagag tcgtgaggtt gcccgtgagc gtgtcaccgg ctggagctcc
     3661 acccggagcg cgatcgacgc cgttgtacac gtagtaatcg atgcgcgcgc ccgcgctcgg
     3721 cagctggatt tcgctcgcgt cgatgccagc cttgaggagc aaacactcga acgggtccgc
     3781 gctgcccgac gcgatcgcca tcttgggaat gtcggcggtg ttgccgtcgc cgtcgaagcg
     3841 gttgcgaggc agtcgcgtaa acgtcgagcc aacggcgttg tcctgacacg acgtgacgct
     3901 cgggatcgtc gtcttgcggc gccacttgcc catctgcacc acgatgggga tgttgcttcc
     3961 ggctgggacg ttcttgaggg tgaacttacc gtcggcgcct gtctgagcaa ccgacacggc
     4021 cgcgaagttt ccggttcccg cgcaggtgtc gcaggtcgcg cccgacgtga acgccggcag
     4081 cgccgcggtt ggatcgaccg ggatgtaaac gtatgcgttg tagagcgggt tcaagcccgc
     4141 cgggtcgtaa acgatgcccg agatcgacgt ggctggcttc gagccgcacg acacgacgtt
     4201 gcaccacagc cctttgcagg gaccggtgcc gcccgacgtg ccggtggtcg gcgtgatcga
     4261 aacgcctgcg tcggtcacgg tgaggggctt tccggagggc aagtccgttg gcgtgccggt
     4321 cacggccgtg caaacggggt cacaggggtt cgcgcacccg cccgtcgtcg agagtagctg
     4381 atagccgttc gagccgagcg cgcggttcgg caagctctgc gcgacgacgc ggttgccttc
     4441 gcaggcgccc cacgttttgc cgtcgcagcg agcgtagccc atcgaacagg tgacgtagtc
     4501 accgctcatc cggaccacgt caccgcactc gacgatcgca ccggcgtcgc tgcacgcgca
     4561 ccctttgtgt cccggtgtcg cgcacgaatc ggtgctcggc aacgagcctt caccgacgtc
     4621 tacgccaccg gcgacgagcg cgctcctgtc cttcttgctg cacgccgcta ccaaaagcag
     4681 aagcaacacg gcgaagcgtc gcgcgagcgt gctccaacgc gtgctctgcg cactagagct
     4741 gtccatcaac ttgcatcgag attgagtcat ggatccacgg accacgcgcg gaattttcct
     4801 atcaagatga cagtctatga aaatcgccgc aagggagctg atgtgcgcgc actcgcacca
     4861 cgttcgaacc cgatgtgcgc tttcgctcgc accgagaacg caccctatct cactcggtgt
     4921 tcgcgtttcg gaacgagccc tcgactgtac gactcgtcac tccgcaaagt tgcgcccgaa
     4981 tcgtcgtcgc gtacgcatcc aaagcacaga aaccgcgatt cgttcacttt tgcggggctc
     5041 acacgcagcc ttggcgcagg atgtgagcgc gcgacgcacg cgacgctcga acgcaccgag
     5101 cgagcgcaca cacaggtggc aatgtgcgcg gaaccgtcac acgaagagcg gcggcgaagc
     5161 cgtgcgccgc gccgaacaac gagatcgctc ggatgattta tcgcttcgcg actcgtgagg
     5221 agttcgcagt cgtcgagcaa ccgaggggca tcgccctgca cgcgagcccc gtagggttct
     5281 ccgaggcatc gcctcagccc cgcgcgtccg cgaactc
//
LOCUS       DASTKS010001223         5380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_642477, whole genome shotgun sequence.
ACCESSION   DASTKS010001223 DASTKS010000000
VERSION     DASTKS010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642477"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VFQ35_26095"
     CDS             <1..1266
                     /locus_tag="VFQ35_26095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9934211.1"
                     /translation="TLEIPVEPVTRHDGAVNFFHTVKTAIRAEDGRVLMTVGVSRNIG
                     ETLELREQLLRSREQLERDRLSTFGAQLPGFLFQLRLAGNGLRFTFVSNRVDYYYGVG
                     PSELTADPARFFARVDDPERVRNELQEALRKGSAQGSAVTHVHKACGSDGAWRWMELL
                     ARAQVEADGSVTFHGYVHDISARHRVEKEREELVEKVSARNQEMEQFTYTISHDLKSP
                     LVTIRGYLGGIEEDIRGGKIERALADIGRVRAAAEKMSRMLAELLEISRVGRIANAPV
                     EVRVADVVAETKELLAARLTGVEVVVDVGDAQVCADRTRLVQVFQNLVENAVKYMGAQ
                     PAPRVTITSRLEGDYVSIAVADNGMGIAPAHHQRIFGLFEKLNARSEGTGVGLALVKT
                     IVELHRGKVWVDSEGDGKGSTFHVRLPCG"
     gene            complement(1352..2536)
                     /locus_tag="VFQ35_26100"
     CDS             complement(1352..2536)
                     /locus_tag="VFQ35_26100"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ricin and poly(3-hydroxybutyrate) depolymerase
                     fusion"
                     /protein_id="HET9934212.1"
                     /translation="MKTSSNESAAVASLLACLALVLAACSSSGASGGGSGGAPSGGAP
                     GSGGASLGNGGSARASGGTSSAYGGASSGGVANLGQGGTVGAGGKAGNMGGRGGATTA
                     GEGGALAASGGKSNGGGASTTGGNASGGSVSGSSGAAGAGGATGSPGCGASDALKSGR
                     ATLDVSGSSREYILKLPDNYDPNHPYKLIFAFHARGGNASQVAGGSNNDYYGLFSRSG
                     GSAIFVSPEGLDAGWRNENGRDIAFVKAMLSAFNAKLCIDQRRIFSTGFSFGGMMSDA
                     IGCAMADVFRAIAPMSGGVPNADHPYSGCDQVNMHPIAVWMAHGDNDTVVPLADGKAA
                     LDIFLKRSQCQTQTSPVSPSPCVAYQGCQADYPITLCQFSGGHNVPSFAAEGIWNFFK
                     QF"
     gene            complement(2888..3211)
                     /locus_tag="VFQ35_26105"
     CDS             complement(2888..3211)
                     /locus_tag="VFQ35_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934213.1"
                     /translation="MNQFVARASVCARGVSVTRDYASFIPRSAFRHMENATASALRER
                     RALNRALPNASGTSFITRNACSQQRFIDVSRVRVAAHERQHRAAKSSRIFTARLRIAL
                     TRMFV"
     gene            3330..4595
                     /locus_tag="VFQ35_26110"
     CDS             3330..4595
                     /locus_tag="VFQ35_26110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9934214.1"
                     /translation="MTQRLLFPLVRVALTASFCSPLLACGGAESSEHTTANAPAVPDS
                     GDVTPNAALPLLGRWEPTAHRAHLAATPVEDSVFDLPEAELARIAEEAEHDLTWYAAD
                     GERYEALFVLEDGTAFGRRGPAPDAPPPNEGNHFGASERIPAVSIEPVEQGGLRLQAG
                     IATDSSDDRRLRTSSTTTLTSYPARTSGAMSGSGNTQSGGCTGTKIGPRAILSAGHCV
                     MNSAGDISFTGRFNPGQTNTTTPNGSIPRSGVYLRDWRVHRKYDYSVAFLQDSSAVVS
                     LGWMGVMWWNSQSGYTGRIAQNLGYPCGPNNGCGAITEQTCKASPRSDKRCDGWMYGH
                     NRPLGSNAFTSDDLLRYDNDVSKGHSGSAIFTTVDDMPNVMAVVTHGVIGEQALGPRF
                     RTSMWNDVCSWIADPNFQSAFATHSLCHP"
     gene            4715..>5380
                     /locus_tag="VFQ35_26115"
     CDS             4715..>5380
                     /locus_tag="VFQ35_26115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934215.1"
                     /translation="MTRPPLVVFAALAAVGLVAACGAAPKAPIAQSSQRVTSNVRSED
                     YVGSKVCVGCHRSEYEAFVASPMHRMTRALPEAEVHAPFAGETFALNGDQARMHQRDG
                     ARWLDLLSRDGSAKTYRLTKVIGGRYREDFVGIEQGRAAEEQILPVSYLIFDSSYRYK
                     GYSVLVTERNRLEAGQVWRQSCIFCHNTPAQLFTLFDELHGPSFPSYQGSTSDDAPGD
                     RRFR"
BASE COUNT          990 a         1776 c         1691 g          923 t
ORIGIN      
        1 accctcgaga tcccagtcga gccggttacg cgccacgacg gcgcggtgaa cttctttcac
       61 accgtaaaga ccgcgattcg cgccgaggac ggccgcgtgc tgatgacggt cggcgtctca
      121 cgcaacatcg gcgagaccct cgagctacgc gagcaactgc tccgctcacg agagcaactc
      181 gagcgcgatc gattgtcgac gttcggcgct cagctaccag gctttctgtt ccagctacgg
      241 ctcgcgggca acgggctccg cttcaccttc gtcagcaacc gagtggacta ctactacggg
      301 gttggcccga gcgagctcac cgcggatccg gctcggtttt tcgcgcgcgt cgacgatccg
      361 gagcgcgttc gcaacgaact gcaagaagcg ctacgcaaag gcagcgctca gggcagcgcg
      421 gtcacccacg tacacaaggc ctgcggctcg gacggcgcct ggcgctggat ggagctcctg
      481 gcgcgtgcgc aggtcgaagc ggacggcagc gtcaccttcc acggctacgt gcacgacatc
      541 tccgcgcgcc accgcgtaga aaaagagcgc gaagaactgg tcgagaaggt cagcgcgcga
      601 aaccaagaaa tggagcagtt cacgtacacc atctcgcacg acttgaagag cccgctcgtc
      661 acgattcgcg gctacctagg cggcatcgaa gaggacattc gcgggggcaa gatcgagcgc
      721 gccctcgccg acatcgggcg tgtccgcgcc gccgccgaga agatgtcgcg catgctcgcg
      781 gagctactcg aaatttcgcg cgtcggacgc atcgcgaacg cgccggtcga agttcgcgtc
      841 gctgacgtgg tggccgaaac aaaagagctc ctcgcggcgc gccttaccgg agtcgaagtg
      901 gtcgtcgacg tcggcgacgc acaagtgtgc gcggaccgga cccggctcgt gcaggtattt
      961 cagaacctcg tcgagaatgc ggtcaagtac atgggagcgc agccagcgcc tcgcgtcacc
     1021 atcacttccc gcctcgaagg cgactacgtc tcgatcgcgg tggccgacaa tggcatgggc
     1081 atcgcgcccg cccaccacca acgcatcttc ggcttgttcg aaaagctcaa cgcgcgctcc
     1141 gaaggtaccg gtgtgggcct tgcgttggtg aagacgatcg tcgagctaca tcgcggcaag
     1201 gtgtgggtcg actcggaggg cgacggaaag ggctcgacct tccacgtccg cctgccttgc
     1261 ggttgaccgc gactcgagcg ggtgcgggcg gcgacgccag ggtccgggcg ttcgcaatga
     1321 cgctgattcg agaggcagtg ctcgactccg ctcagaattg cttgaagaag ttccagatcc
     1381 cttccgcggc gaagctcggc acgttgtgcc ctcccgaaaa ttggcacagc gttatggggt
     1441 agtcggcttg gcatccctgg taagcgacgc acgggctcgg gctaaccggg ctcgtctggg
     1501 tctggcactg gctgcgcttg aggaagatgt cgagagcggc cttgccgtct gcgagaggaa
     1561 cgaccgtgtc gttgtcgcca tgtgccatcc agaccgcaat cgggtgcatg ttgacctggt
     1621 cgcagccact gtagggatgg tcggcgttcg gaactccgcc cgacatggga gcgatggctc
     1681 gaaacacgtc cgccatcgcg cagccaatcg cgtccgacat catccccccg aagctgaaac
     1741 cggtggagaa gatgcgccgt tggtcgatgc agagcttcgc attgaaggcc gagagcatcg
     1801 cttttacgaa cgcgatgtcc cgaccattct cgtttcgcca acccgcatcg aggccctcgg
     1861 gtgagacgaa aatggcgctg ccaccggagc gactgaagag cccgtaatag tcgttgttcg
     1921 agccgcctgc gacctgagat gcgtttccac cgcgcgcgtg gaaagcgaag atcaacttgt
     1981 aggggtgatt cggatcgtag ttgtccggaa gcttcaagat gtactctctc gaactgccgg
     2041 aaacgtcgag cgtcgcgcgc ccgctcttca gcgcgtcgga ggcgccacac ccagggcttc
     2101 ccgtggcacc tcctgctccc gcagcgcccg acgacccaga aacactaccg ccggaagcgt
     2161 ttccaccagt cgtgctcgcc ccacccccgt tggatttgcc tccgctcgcg gctaaagcac
     2221 cgccctcgcc agccgtcgtc gcgccgcctc ggccgcccat atttcccgcc ttacctccgg
     2281 cgcccacggt gcccccttga ccgaggttcg ccacgccacc gctggaggca ccgccgtacg
     2341 cactgctcgt accaccgctc gctcgagccg aaccgccgtt gccaagcgag gcgccaccgg
     2401 agcccggagc accgccgctc ggcgctcccc cactgccgcc gccacttgct ccgctgctcg
     2461 agcacgctgc caagaccaga gccaaacaag cgagcaaact cgcgaccgct gcgctttcgt
     2521 tggatgaagt cttcatcgga acctttgttg acgcacgtga gcccaatcgg ggcttccaca
     2581 aacgaccggc gctgtgcgca cgcgccgatt tcgagcgtca ccgcgcgcgc ccacgcagcg
     2641 acgatgcatc actatagaga tacggcgcta aacagggaag ctactatagt agcgattctc
     2701 cacatttgag tgagccgctc accctttcgg tagagcacga cgtccaatcg cactgattga
     2761 ttcccgccgt aatgcagtcg agtgagaagc ggcgcccgca caccccaaag tgcgactgcg
     2821 gcgacgaggg cattcgacta tacgcgcgct cgttaaagga ccgagtcttc tgagtcgagg
     2881 ccgcggatca aacgaacatg cgcgtgagcg cgatcctcaa ccgcgcggtg aagatgcggc
     2941 tcgacttggc agctcggtgt tggcgctcgt gcgcggccac tcgcactctc gagacgtcga
     3001 tgaagcgttg ctgtgaacaa gcgttgcgtg tgatgaagct ggtgccgctc gcgttgggaa
     3061 gcgccctgtt caaggctcgg cgctctcgga gagcactcgc ggtcgcattc tccatgtggc
     3121 ggaacgcgct gcgcggaatg aacgaagcgt agtcgcgtgt aacgctgacg ccgcgcgcac
     3181 agacgctcgc gcgggcgaca aattggttca tatgacggcg acgacacttg cgcggcgagg
     3241 tccgacagaa atcaaatgcc tattgtgcga ggccctacga aaagaggatt gtagggcccg
     3301 aaacggcggg ggagcgaaag gccatgtcca tgactcaaag gctgctattt ccgttggtgc
     3361 gcgtcgcgct caccgcctcg ttctgctcgc cgctgctcgc ttgcggtggt gcggaatcgt
     3421 ccgagcacac cactgcgaat gcacccgccg tgcctgattc gggcgatgtg acgcccaatg
     3481 cagcgctgcc gctcttgggg cgctgggagc ccaccgcgca ccgcgctcac ttggcggcca
     3541 cgccagtcga ggacagtgtc ttcgatctgc ccgaggctga gctcgcacgc atcgccgaag
     3601 aggccgagca cgatctcacc tggtacgcgg cagacggcga acgctacgag gccctcttcg
     3661 ttctcgagga cggcactgcc tttggccggc gcgggccggc gcccgacgct ccaccgccga
     3721 acgagggcaa tcattttggt gcgtcggagc gcatccccgc cgtttccatc gaaccggtgg
     3781 aacaaggtgg tttgcgcctg caagcgggaa ttgccaccga ttcgagcgac gatcgccgat
     3841 tgcggacttc gagcaccacg acgctcacgt cctatcccgc gcgcacgagc ggcgccatga
     3901 gcggaagcgg caacactcag agcggaggct gcaccggtac caagatcgga cctcgggcca
     3961 ttctctccgc ggggcactgc gtgatgaact ccgcgggcga catctcgttc acgggccgct
     4021 tcaaccccgg gcagacgaac acgacgactc ccaacggttc gatcccgcgt agcggcgttt
     4081 atttgcgcga ctggcgcgtg caccgtaagt acgactactc cgtcgccttc ctgcaggact
     4141 cttccgcggt ggtcagcctc ggttggatgg gcgtcatgtg gtggaacagt cagtctggct
     4201 acaccggccg catcgcgcag aatctgggct acccatgcgg ccccaacaac ggctgcggcg
     4261 cgatcacgga gcaaacctgc aaggcgtctc cgcgcagtga caagcgctgc gacggctgga
     4321 tgtacgggca caaccgtcct ctcggctcga acgctttcac gagcgacgac ctgctccgct
     4381 acgacaacga cgtttcgaag gggcacagcg gcagcgccat cttcaccacc gtcgacgaca
     4441 tgccgaacgt aatggccgtc gtcacccacg gggtcattgg cgagcaagcg ctgggcccgc
     4501 gcttccgcac ctcgatgtgg aacgacgtgt gctcgtggat cgccgatccg aatttccaaa
     4561 gcgcatttgc gacccactcg ctctgccatc cctgaggctc gtcaccacgc atcggcgtga
     4621 accggcgtgg ctcgaccgca aatgctttct ggggtgggcg gcggcgggag tgaccccgta
     4681 gcctccgctg ccacgggcga gtaccctacg cgccgtgact cgcccgcccc tcgtcgtctt
     4741 cgccgcgctc gcggccgtgg gactagtcgc ggcttgcggc gcggcaccga aggcgccgat
     4801 tgcacagagc tcgcaacgcg tcactagcaa cgtgcgcagc gaggactacg tcggttccaa
     4861 ggtgtgcgtc ggatgtcacc gcagtgagta cgaagcgttc gtcgcttcgc cgatgcaccg
     4921 tatgacgcgc gcgctgccgg aagccgaggt ccacgcgccg tttgcgggag agacgtttgc
     4981 gttgaatggc gaccaagctc gcatgcacca gcgtgacggc gcgcgctggc tcgacctcct
     5041 gtcgcgcgac ggcagcgcga agacgtatcg cctgaccaag gtgatcgggg ggcggtatcg
     5101 cgaagacttc gtgggcatcg agcaaggccg agccgccgaa gagcagatct tgccggtgtc
     5161 gtacctgatt ttcgattcga gttaccggta caaggggtat tcggtgctcg tcaccgagcg
     5221 gaaccgcctc gaagcgggtc aggtctggcg acaatcgtgc atcttttgtc acaacacccc
     5281 cgcgcagctc ttcacgctct tcgacgagct ccacggccct tcgtttccga gctaccaggg
     5341 ctcgacgagc gacgacgcgc cgggggacag gcgctttcgc
//
LOCUS       DASTKS010001224         9632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_197789, whole genome shotgun sequence.
ACCESSION   DASTKS010001224 DASTKS010000000
VERSION     DASTKS010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197789"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFQ35_26120"
     CDS             <1..673
                     /locus_tag="VFQ35_26120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934216.1"
                     /translation="ALDPDLLNRPRVREQPAKDAPFARETAREAQAVSKQSGVPAPSA
                     PPAAASPARAERTVPLLELGPLVSLGAIPSPSFGASVSGGFERGRWRAFVTGAFFPSV
                     RKELPRTNAAAAFELRSLSAALCWLVPLSPRWGLGPCAATEFGSVAARGEGVDFPAGE
                     TKRWGATGAGGMVRFESQRSFEVGLQGIVAVPWGRPTFRVAQVEAYQPAVLTLGLRAF
                     AGVRF"
     gene            726..1304
                     /locus_tag="VFQ35_26125"
     CDS             726..1304
                     /locus_tag="VFQ35_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9934217.1"
                     /translation="MAIRHFARPVPSAASATEPPELGEIYEAHFDFVWRSLRRLGVPE
                     AEVEDAVHDVFLIAHRKLPEFEGKSTHKTWLFAIALRVAQNQRRSLGRRRVADVDADA
                     VADAEASPDVQTERSRGIELGMRLLNELDQDKRVVFILAELEQLPASEIGAALGIPLF
                     TVYSRLRAARRAFEAALARERARGAWRSRWTS"
     gene            1292..2071
                     /locus_tag="VFQ35_26130"
     CDS             1292..2071
                     /locus_tag="VFQ35_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934218.1"
                     /translation="MDELSKAASELVSEMRRGGAASEGARERVRQRLTVSLSGASAAA
                     VGAASASATVKASTAAAQAATVVSVSKLAFLVALGALGGSAVLTPVALLSAPPATSIQ
                     RVSPAPTPHAQSRGVSFDPAQDEVRRGALASQPSPVDAPLATARAVHEPTPLSPIPPR
                     ATLRPSVPEFENNLSAETELLARVRERLRAGSGAESLALLAEHARRFPNGVLAEEAAA
                     SRVLALCSLGRTADGDSEKARFYRTYPHSVLGPRLERACAR"
     gene            2158..2982
                     /locus_tag="VFQ35_26135"
     CDS             2158..2982
                     /locus_tag="VFQ35_26135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934219.1"
                     /translation="MISRVLPIVFVFGVGCGFGGRVELGDNGGAPPFLDDGSGGVAAG
                     GVRASGGAAVSAQGGAVVLAQGGAAVSSGAGGAPSTSVAHPMLPMDATGEVPLSSNGY
                     GIRGKWYAFSDEIDGGQTRIDGYGPGFVPFVPGRGMCIHGTTTSGLVDNWKTWGAGIG
                     IALNVVDGTAETLVNPPPCYSLVISSDAEYPSELRADLNPTDDGTKQPANVELHAGSN
                     DFCLSDAKTFAHCTPELDCLTPDSLNQGLAKINVVAATGTVSGPISFCIEALIPHD"
     gene            2975..4222
                     /locus_tag="VFQ35_26140"
     CDS             2975..4222
                     /locus_tag="VFQ35_26140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9934220.1"
                     /translation="MTEPRRLPLTPKQRVFLPLAAEARPQDLSARPRVAVWELTLRCD
                     LSCAHCGSRAGKPRDRELSTEECLRLVDELAALSVLEVSLIGGEAHLHPGFLPVLRAL
                     KAHGIEVGLTTGGRRVDDALARAMAAAGLDSVSVSIDGLREAHDTQRALVGSFDAALT
                     SVRAFRDASVFVSVNTQINRISSNDLEALFELIVERGARAWQIALTVPMGRAADRPDW
                     LLQPSDLLDLFPRLAALKQRCDDARVRLWPGNNVGYFGPYEHVLRGQMPLGHAYSCGA
                     GVTTLGIESDGTIKGCPSLPSEAWVGGNVREHSLKEIWERAPRLRYIRDRTASTLWGF
                     CADCYYADECRAGCTWTSFSFFGRAGNNPYCHHRALELAARNQRERLIRVEAPPGEPF
                     DTGRFEVVLEPRSSANSPVEGLQ"
     gene            4219..4629
                     /locus_tag="VFQ35_26145"
     CDS             4219..4629
                     /locus_tag="VFQ35_26145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934221.1"
                     /translation="MSSRLLPCSHCSRHVRIGSESCPFCHAPSAPVAPAARAAASPAR
                     LSSLAIMTFRAATLGVAITACGGESNDPEGHGGATGSGGMSAAGGDVSNGGVTDTGAG
                     GTPSSGGRGEVGSGGRTDGSGGGPVPIYRATPRG"
     gene            4733..6268
                     /locus_tag="VFQ35_26150"
     CDS             4733..6268
                     /locus_tag="VFQ35_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9934222.1"
                     /translation="MKGKTTMTQSTLAFEANSFFDFSRAPAPELSKEHWEFRLLKSGP
                     DVDPEEVELASVTSIEVLVMWGRNVLSVSHLTPPRDFFVGELDDAEHACDVFVPAEKT
                     GQSRFSLVRVEHGACSVVKPAGARGFIQDERGRRALDSERTEQVALTERTRVEFELGG
                     FVFHVASVNAGRPLKKGLGVSWDWTVASFFGLSFLTHMGMLAALAFFVPNLSGITDED
                     TDRDRLYVLQQYLDASAERERERQLTEAPSPGEQEGGTGQQAAGESGSMGKPTASVAS
                     KRYAVKGPKDNPDPNLSHDAAVHEAATFGMISLLQGDPDAPTAPWVRDASLGSDEVSA
                     QGNMWGDEIGVAFGLGGLGPSGVGEGGGGRGRGVGMGEIGTIGHGSGHGDGQGWGNGH
                     GQLGRGHVSKSPGALRPGVTTVSGRLPPEVIQRVVRQNFGRFRACYQTGLSRNPNLEG
                     RVSARFVISRDGAVSNVANGGSDLPDSAVVSCVISAFYGLSFPEPENGIVTVTYPIML
                     SPG"
     gene            6400..7545
                     /locus_tag="VFQ35_26155"
     CDS             6400..7545
                     /locus_tag="VFQ35_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934223.1"
                     /translation="MIASQPMVAPALELEVATPRGSGKLGRAGEFLVVGGATLPLLPL
                     ALLLRRVFGLDSAELAVGFVAFHAAHVINDPHFAVTYLLFYRNVRERAFSRAFSLRQR
                     ARYWIAGVGVPVLLGGFTLFALARHSAEKVGYLLQLMFLLVGWHYVKQGFGVLTVISA
                     RHGVRLSTGERRAFLAHCVSAWLFARANPRDFGHEASSDGVYFMTFPHPPGLELATRV
                     AFAASALGLGFVLVRKWRREGRLPPLAALVGFLVTVWLWTAFSRIDPLLVYVIPALHS
                     IQYLYFVWLSRRNEAVEKCGPPAFESVGKAMFRLSLGGIVLGWFLFRGLPPLLDEYLV
                     LKDPFDPLGETPYFAALGTFVNIHHYFMDSVIWRRENPETRYLLTRA"
     gene            7836..>9632
                     /locus_tag="VFQ35_26160"
     CDS             7836..>9632
                     /locus_tag="VFQ35_26160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9934224.1"
                     /translation="MNERVELSDSFLAAMFRALTSQSPDYVSCLDRERRVLYLSRTLT
                     RDLPSILGRKTETFVTPEHREESIRCTEAAFVTGEPQRMEIDVTLAGGGSHAFEIRTI
                     PFKGPQGEPLAVQITTDLTERRRLMAQLVESEGFRTLIVENLPDYVTLLDRDFRFRWL
                     NRAAPGLTREQLIGANVLDYVAERHRALVRSNVELAFEKGQPTQYEVEAYGDGVQPAW
                     YVTRVIPVASQTPLQNVLTITSDVTVRKRAELALRASEERFRALAERSPDVICIIDRR
                     RVIEYVNRLPMEASEDEVLGHRLDEFARLEELPRLRSALESVFTSGQSTEVELTSPRG
                     ETYRVRAARFDWVEGGEHALVVSTDITRQRADELARESLQAQLHQAQRRESVGMLAGG
                     IAHDFNNLLQVIQTNLHFAREMLRPTGLVLDELGEALRATERAAELTTHLLAIGRRQR
                     VNPKNVDLSLLIEASLRMLRRVIPESIELSFEPSPVPCTVSADPPQLEQVLLNLCLNA
                     RDAMPHGGHLKVEVGVVDSKPPGARFCVTDSGAGIAQDVLKQIFEPFFTTKGAGSGLG
                     LAVAAGIVAAHGGTISVTSEVGKGSTFCVDLPL"
BASE COUNT         1524 a         3084 c         3245 g         1779 t
ORIGIN      
        1 cgcgctggac cccgacctgc tgaatcgacc gcgcgtccgt gaacagccgg cgaaggacgc
       61 gcccttcgcg cgcgaaactg cgcgcgaagc tcaggctgtt tcgaagcaat ccggcgtccc
      121 tgctccttcc gcgccgcccg cggccgcgag cccagcgcgc gccgagcgca ccgtgccgct
      181 gctcgagctc gggccgctcg tcagtctcgg agcgatccca tcgccttcgt tcggcgcctc
      241 cgtgagtggc ggtttcgaga gaggccgatg gagagcgttc gtcacgggtg cgttctttcc
      301 atcggtccgg aaagaactac cgcgaacgaa tgcggcggca gctttcgagc tgcgctcgct
      361 ctccgcggcg ctctgctggc tcgtgccgct gtcaccgcgc tgggggcttg gcccgtgtgc
      421 ggctaccgag ttcggtagcg tagcggcacg aggtgagggc gtggactttc ctgccgggga
      481 gaccaagcgc tggggagcga ccggtgccgg cggcatggtg cgcttcgaaa gccagcgctc
      541 cttcgaagtc gggctgcagg gcattgtcgc cgtgccctgg ggcagaccga cttttcgagt
      601 ggcccaggtc gaagcgtacc agccggcggt gctgaccctt gggctgcgcg cttttgcagg
      661 ggtgcgcttt tgaaccactg ccgattcggt gacaagtctc cgaacccgcg cacactaagc
      721 cgagcgtggc gatccgtcac tttgcgcgcc cagtcccttc agcggcttcg gctaccgagc
      781 cgccggagct cggggaaatc tacgaagcgc atttcgactt cgtctggcgc agtctccgcc
      841 gcttgggcgt gcccgaggcg gaggtcgagg acgctgttca cgacgttttt ctgatcgcgc
      901 atcgaaagct ccccgagttc gagggcaaga gcacgcacaa gacatggctc ttcgcgattg
      961 cgttgcgcgt cgcgcaaaac cagcgtcgct ccctcgggcg ccgtcgggtc gccgacgtcg
     1021 acgcggacgc cgtcgctgac gcggaggcgt cgcccgacgt ccagaccgag cgcagtcgcg
     1081 gcatcgagct tggcatgagg ctcttgaacg agctcgacca agacaagcgg gtcgtcttca
     1141 ttttggcgga gctcgagcag ctgcccgctt ccgagattgg cgcggcgctc ggtatcccgc
     1201 tgttcaccgt atactcgcgg ttgcgcgcgg ctcgtcgggc tttcgaagct gcgctcgcgc
     1261 gagaacgagc gcgcggcgct tggaggagtc gatggacgag ctgagcaaag cagcgagcga
     1321 actagtgtcc gaaatgcggc gaggcggagc cgcctccgaa ggcgcgcgcg aacgggtgcg
     1381 gcagcggctc accgtttcgc tcagcggggc ttcggctgcg gcggtcggag cggcgagcgc
     1441 gagcgcgacc gtgaaggcgt ccacggcggc ggctcaggcg gcaacggtgg tctccgtgtc
     1501 gaagctagcg ttcctggttg ccctcggagc gctcgggggc agcgccgtcc tcacgccagt
     1561 cgccttgctg agcgcgccgc ccgcgacgag cattcagcgg gtgagcccgg cgccaacgcc
     1621 gcacgctcag tcgcggggcg tatccttcga cccggcccag gacgaggtgc gccgcggcgc
     1681 tctcgcgtcg cagccgagcc cagtcgacgc accgcttgcg acggctcgag ccgtacatga
     1741 gccgacgcct ctgtcgccga ttccaccgcg cgcgacgcta cgccccagtg tccccgagtt
     1801 cgagaacaac ctttctgccg aaacggagct gctcgcacgg gtgcgtgaac ggcttcgggc
     1861 ggggagcggg gcggagagct tggcgctctt ggcagaacac gcgcggcgct tcccgaacgg
     1921 cgtgctcgcg gaggaggcgg ccgcgagtag agtgctggca ctttgctccc tgggtcggac
     1981 cgcggatgga gacagcgaga aagctcgctt ctaccgcacg tacccgcatt ccgttttggg
     2041 gcctcgcttg gagcgggcgt gcgcaaggtg agcgctgctt tctctgacaa gttcaccgcc
     2101 tgctggtcac gaagcagtat gaacttcgct cgtcgcgctc tcgcaaggag gcatgcggtg
     2161 atctctcgag tgctcccgat cgtcttcgtt ttcggcgttg ggtgcggctt tggcggccgc
     2221 gtcgagctcg gcgacaatgg cggcgcgccg ccattcctcg acgatggctc cggcggggtc
     2281 gcggcgggtg gcgtccgagc gagtggtggc gccgccgttt ccgcgcaagg cggagcggtg
     2341 gtgcttgcac aaggaggcgc agcggtgtcg agcggggcgg gaggagcgcc ctcgaccagc
     2401 gtcgctcacc cgatgctgcc gatggacgcg acgggtgaag ttccactttc gagcaatggg
     2461 tacgggattc ggggcaagtg gtacgccttc tcggacgaga tcgacggcgg ccaaacgcgt
     2521 atcgacggct atggccctgg cttcgtgcct ttcgttccgg gacgcgggat gtgcattcac
     2581 gggaccacga cctcgggact cgtcgacaat tggaagacgt ggggggcagg tatcggcatc
     2641 gcgctcaacg tcgtcgacgg caccgccgag acgttggtga atccgccgcc gtgctacagc
     2701 ctggtcatca gcagcgacgc ggagtaccca tccgagctgc gcgccgactt gaacccaacc
     2761 gacgatggca cgaagcagcc agcgaacgtt gagctccatg cgggttcgaa cgacttctgt
     2821 ctgagcgacg ccaagacgtt cgcccattgc actcccgagc tcgactgcct cacccccgac
     2881 tcgttgaacc aggggcttgc caagatcaac gtggttgctg caaccggcac cgtgtcgggg
     2941 ccgatctcgt tctgcatcga ggccctcatt ccgcatgact gagccacgaa ggctgccgct
     3001 cacgccgaaa caacgcgtat ttctgccgct cgcggcggaa gcgcgcccac aggatctaag
     3061 tgcccgcccg cgcgtcgcgg tgtgggagct cacgctgcgc tgtgatctga gttgtgcgca
     3121 ctgcggatcg cgcgccggaa agcctcgcga ccgcgagctg agcacggaag aatgcttgcg
     3181 gctcgtcgac gaactcgcgg cgctctcggt actcgaggtt tcgttgattg gcggcgaggc
     3241 gcacttgcat cccggcttct tgccggtgct gcgcgcactc aaggctcacg gaatcgaggt
     3301 ggggctcacg accggcggtc gccgcgtcga cgacgccctg gctcgcgcga tggcagccgc
     3361 ggggctcgat agcgtcagcg tttcgataga tggtttgcgc gaagcccacg acacccagcg
     3421 cgcgctcgtc ggatccttcg atgccgcgct cacgagcgtt cgggcctttc gcgatgcgtc
     3481 cgtgttcgtt tcggtcaaca cccaaatcaa ccgcatcagc tcgaatgact tggaagctct
     3541 gttcgagctc atcgtcgagc gtggagcgcg tgcttggcaa atagctctga cggtcccgat
     3601 ggggcgcgcg gcggatcgcc cggattggct tctgcaaccg tctgacttgc tcgacttgtt
     3661 tccgcgcctg gcggccttga agcaacgctg tgacgacgct cgcgttcggc tctggcctgg
     3721 caacaacgtc gggtacttcg gtccttacga gcacgttttg cgcgggcaga tgcccttggg
     3781 gcacgcctac tcgtgtggcg ccggcgttac gacgcttgga atcgagtcgg acggaaccat
     3841 caagggctgc ccctcgctgc cgagcgaagc ctgggtcggc ggcaacgtgc gcgagcactc
     3901 gttgaaagag atttgggaac gggcgcctcg gcttcgctac attcgtgacc gcactgcgag
     3961 caccttgtgg ggcttctgcg cagactgcta ctacgccgac gagtgccgcg cgggctgcac
     4021 gtggacgtcg ttcagtttct tcgggcgagc aggcaacaac ccgtattgcc atcaccgcgc
     4081 gctcgagctc gcggcgcgca atcagcgcga gcgcttgatc cgcgtcgaag cgccgcccgg
     4141 tgaaccgttc gatacgggtc gcttcgaagt cgtgctggag cctcgttcca gcgccaattc
     4201 acctgtggag ggattgcaat gagttcgcgt ctacttccgt gctcgcactg ttcgcgtcac
     4261 gttcgcatcg gctccgaaag ctgcccgttt tgtcatgcgc cgagcgctcc ggtcgcgccc
     4321 gcggcgcgcg ccgcagcttc gcccgcgcga ctatcgagcc tcgcgatcat gacgtttcgc
     4381 gccgcgaccc tcggcgttgc catcaccgcg tgcggcgggg agtcgaacga tcccgaaggc
     4441 cacggcggcg cgacgggcag cggcggaatg agcgccgctg gcggcgacgt atccaatggt
     4501 ggagtcaccg acacgggcgc aggtggcacg ccctcgtctg gcggccgcgg cgaagtcggg
     4561 agcggcggtc gcaccgacgg ctcgggtggc ggccccgtgc ccatctatcg cgcgacgccg
     4621 cgcggctgag ctgcgtccta cgcatcggcg tcggtgaacc ttcctcgcgc gttcgtcgcg
     4681 ggagaagggg caccctcgcc ggaactttca ctctgcgtgg tggtcagtag cgatgaaggg
     4741 caagaccacc atgacacagt ccacgttggc attcgaagcg aattcttttt tcgactttag
     4801 tcgcgcaccc gctcccgagc tctcgaaaga gcactgggag ttccggctgc tgaagagcgg
     4861 ccccgacgtg gatcccgagg aggtcgaact cgcctcggtc acgtcgatcg aagtgctggt
     4921 gatgtggggg aggaacgttc tctcggtgtc ccacctgaca cctccgcgcg acttcttcgt
     4981 cggtgagctc gacgacgccg agcacgcctg cgatgtcttc gttcccgcag aaaagaccgg
     5041 ccaatctcgc ttttcgctgg tgcgggtgga acatggtgca tgctccgttg tcaaacctgc
     5101 tggtgcccgc ggtttcattc aggatgaacg aggccgtcgg gcactcgaca gcgagcgcac
     5161 cgagcaagtc gcgctcaccg agcgcactcg agtcgagttc gagctcggtg gtttcgtgtt
     5221 tcacgtcgcg agcgtcaacg ctggccgtcc gctgaagaag ggcctgggcg tttcgtggga
     5281 ttggacggtc gcgtctttct tcgggctctc gttcttgact cacatgggca tgctcgcggc
     5341 gctcgcgttc ttcgtgccga acctgtcggg catcacggac gaagacacgg atcgcgaccg
     5401 cctctacgtg ctgcagcagt acctcgacgc ctccgccgag cgggaacgcg agcgtcagct
     5461 gacggaagcg ccgagcccgg gtgagcaaga aggtggcacc gggcagcaag cggcgggtga
     5521 gtccggctcg atgggtaagc ccaccgcgtc cgttgctagc aagcgttacg ccgtgaaagg
     5581 tccaaaggac aatcccgatc cgaacttgtc gcacgacgcc gccgtccacg aagccgcgac
     5641 cttcgggatg atctcgctcc tgcaaggtga tcccgacgca ccgacggcgc cgtgggtgcg
     5701 cgacgcatcg ctcgggagcg acgaggtcag cgctcagggc aacatgtggg gtgacgagat
     5761 tggagtcgcg tttggtctcg gcggtctcgg cccgagcggc gttggagaag gcgggggagg
     5821 ccgcgggcgc ggcgttggga tgggtgagat cgggaccatc ggacatggta gcggccacgg
     5881 cgacggtcaa ggctggggga acggtcacgg gcagctcggg cgcggccacg tgagcaagtc
     5941 gccgggcgcg ttgcggccag gcgtgactac ggtgagtggt cgcttgcctc ccgaggtgat
     6001 ccagcgcgtc gtccggcaga acttcggtcg ttttcgcgcc tgctaccaga cagggctatc
     6061 gcgcaatccc aacttggaag gtcgcgtatc ggcgcggttc gtcatcagcc gcgacggggc
     6121 cgtatcgaac gtcgccaacg gcggctcgga cctgcctgac tccgccgtcg taagctgcgt
     6181 catttcggcc ttctatgggc tgagcttccc ggagcccgag aacggaatcg tgacggtgac
     6241 ctaccccatc atgctctctc ccggctgaag ccgtcccgca agtgcgatgt cgagcgccgc
     6301 gcgaaggccc gcgtgcggcg ctcgaggggc ggcggggcgt cgtcggaaaa tcacactcgt
     6361 gcaagatcgc tgctcggacg actacagccc cttcggcgca tgatagcgtc tcagccgatg
     6421 gtggctccgg cgctcgaact cgaagtcgct acccctcgag gcagcggaaa gctcgggcga
     6481 gccggggagt ttctcgtcgt gggcggcgca acgctgccgc tcttgcctct cgcgctgctc
     6541 ttgcgccggg tattcgggct cgacagcgcc gagctcgcgg tcgggttcgt cgcgttccac
     6601 gccgcacacg tcatcaacga tccgcacttc gccgttacgt acctcctttt ttaccgaaac
     6661 gttcgcgagc gagcgttcag ccgcgcgttt tcgctccgac aacgcgctcg ctattggatt
     6721 gccggcgtcg gcgttcccgt ccttctgggt ggcttcacac tgttcgcgct cgcgcggcac
     6781 tcagcggaga aggtcggtta cctgttgcag ctcatgttcc ttttggtggg ctggcactac
     6841 gtaaagcagg gcttcggggt tttgacggtc atttcggcga ggcatggagt gcggctttcg
     6901 accggggagc gacgagcctt ccttgcccac tgcgtctcgg catggctgtt cgctcgcgcc
     6961 aatccccgcg acttcgggca cgaagcgagc tcggacggtg tctacttcat gacctttccg
     7021 catcctccgg ggctcgagtt ggcaacgcgc gtagccttcg cggcgagcgc gctcggactc
     7081 ggcttcgtgc tcgtgcgcaa atggcggcgc gaagggcgct tgcctccgct cgcggcgctg
     7141 gtcggttttc tcgtaacggt ctggctctgg actgccttct cgcgaatcga tccgctcctc
     7201 gtttacgtga ttccggcgct gcactcgatt cagtacttgt acttcgtgtg gctttcgcgg
     7261 aggaacgagg cggtcgaaaa atgtgggccg cccgcgttcg aatcggttgg aaaagcgatg
     7321 ttccgcctat cgctcggtgg catcgtgctc ggctggtttc tctttcgagg gctgccgccg
     7381 ctgctcgacg agtacctcgt tttgaaggat ccgttcgacc cgctcggtga gactccgtac
     7441 ttcgcggcgc tcgggacctt cgtgaatatc caccactact tcatggattc ggtgatttgg
     7501 cgtcgcgaaa acccagagac gcgatattta ctgacgaggg cgtgagcatg gagcccgcga
     7561 atggacaagt cagtcgcgag cgagccgaga ccgcggcgag gcgggcgcgt gaggcgcgag
     7621 cactgcacgc cctctgaagt tactaaagtc tcaggattta tcggatctcc gaaccacgca
     7681 tcggcccgcc gctttcgggg tttctggtgc ccgctctggc cttggcgttt cgccacctat
     7741 cgaatgagcc gagcgcaccg aaaaaaggga tagagcacgg tctctcggcg gatttcgacg
     7801 ccgcgtcagg cgagagacgg tgtagattgc tgcccttgaa cgagcgcgtc gagctctccg
     7861 actccttcct cgcggccatg tttcgcgcgc tcacgtcgca gtcaccggac tacgtgagct
     7921 gcctcgatcg agagcgccgc gtgttgtacc tgagtcgaac actcacgcgc gacttgccga
     7981 gcattctcgg tcgcaaaacg gagaccttcg taacaccgga gcaccgcgaa gaatccatcc
     8041 gctgcacgga agcggccttc gtcacggggg agccgcagcg catggagatc gacgtcacac
     8101 tcgctggcgg cggttcgcac gcattcgaga tccgcaccat cccgttcaag ggcccgcagg
     8161 gagagccgct cgcggtgcag atcacgaccg acctcaccga acggcggcga ttgatggcgc
     8221 agctcgtcga gagcgaaggc tttcgaacgc tgatagtcga gaaccttccc gattacgtga
     8281 cgctgctcga tcgcgacttt cggtttcgtt ggctcaaccg ggccgcgccg gggctcacga
     8341 gggagcagtt gattggcgcg aacgtcttgg actacgtggc cgagcgacac cgggcgctcg
     8401 tgaggtccaa cgtcgagctc gctttcgaaa aggggcagcc gacgcaatac gaggtcgagg
     8461 cgtacggaga cggggttcag ccagcctggt acgtgacgcg tgtcattccg gtcgcctcgc
     8521 agactccact gcagaacgtc ttgaccatca cgtccgacgt gaccgtgcga aagcgcgccg
     8581 agctcgcgtt gagggcgagc gaagaacgct ttcgtgccct cgcggagcgg tcgccggacg
     8641 tcatctgcat catcgatcgg cggcgtgtga tcgagtacgt gaaccggctt ccgatggagg
     8701 cgtccgagga cgaggtactc ggccatcgcc tcgacgagtt cgcccggctc gaagagttgc
     8761 cgcgtcttcg cagcgcactc gaatcggtgt tcaccagcgg acagagcacc gaggtcgagc
     8821 tcacttctcc caggggcgaa acttaccgtg tgcgagcggc acgcttcgac tgggtcgagg
     8881 gcggtgaaca cgcgctcgtc gtttcgaccg acataacgcg gcagcgcgcc gacgagctcg
     8941 cccgcgagag cctgcaagcg cagcttcatc aagcccagcg acgtgagagc gtcggcatgc
     9001 tcgcgggcgg catagcgcac gactttaaca acttgctcca ggtgattcaa acgaacctcc
     9061 acttcgcgcg cgagatgctg cgcccgaccg ggctcgtgct cgacgaactc ggagaggcgc
     9121 tgcgcgcgac ggagcgcgcg gcagagctca ccacccatct gttggcgatt ggccgtcgtc
     9181 agcgcgtgaa cccgaagaac gtcgatctct cccttttgat cgaggcgagc ctgcgcatgt
     9241 tgcggcgggt aattccggag agcatcgagc tatctttcga gccgagcccc gttccgtgta
     9301 cggtgtcggc agatccccct cagctcgagc aagtgctgct caacttgtgc ctgaacgcgc
     9361 gggacgccat gccccacggc gggcatctca aggtcgaggt gggcgtcgtc gactccaagc
     9421 ctccgggcgc gcgtttctgc gtcaccgaca gcggcgccgg catcgcgcag gacgtcctga
     9481 agcagatctt cgagcccttc ttcacgacca agggggctgg gtcgggtctc ggtctggccg
     9541 ttgccgccgg tatcgtcgcg gctcatggtg ggaccatctc cgtgacgagc gaggtgggga
     9601 aaggctctac cttctgcgtg gacctgccgc tc
//
LOCUS       DASTKS010001225         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1760698, whole genome shotgun sequence.
ACCESSION   DASTKS010001225 DASTKS010000000
VERSION     DASTKS010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760698"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFQ35_26165"
     CDS             complement(<1..589)
                     /locus_tag="VFQ35_26165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934225.1"
                     /translation="MTTPLEPAPCLRYMVVVSSAAKPGTIERYRRNRQDEIDSAAQYH
                     AMADGERDQRTARLYRALAAMEEKHAAFWEDRLRKENASVPPPSPSFRARVLSFVARR
                     FGARTVLPTVATREYQDRDVYRAQPETQGTKMVAQEWSHARVLQSLLVEHAEGAPGAE
                     VARVERQHRRVGGNALRAAVLGANDGLCSNLALIMG"
     gene            670..>2464
                     /locus_tag="VFQ35_26170"
     CDS             670..>2464
                     /locus_tag="VFQ35_26170"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934226.1"
                     /translation="MQVSTFPDGHQVKKQKNSRDVFEQALVLESRGRAEEAAAKYRAL
                     ISRNAEHDRALFRLGLSLLGKGEVEAATRYLERAVEVRRDEPTYLLSLGEAYRLQGKV
                     ELAGAVLTYAVNIDPDLTEAHEKLAPIRMHAGAYEEALSHLEKVAALRPDDPLAHVGL
                     AWALLLLNRPAQALARAERAIELAPNLAGAHRCAGDSLDMLGDKQRSLESYRRVLELD
                     PGDSVAHSNLIISMLTEPSHDAKAVLAEARAWEAQHALPLRAHQREHQNVRDPDRRLR
                     IGYVSPDFRAHPVQQFLVPLLQRHDPNAVEIFLYSSVERADARTEWYQGFAGDRFRDI
                     RPLSDVEAAELVRRDQIDVLVDLAVHGPGNRLRVFACKPAPIQMTWLGYAGTTGLASI
                     DYRITDPYLDPLDADLSVYSESSLRLPATFWSYDALEVNLVVNPAPAVERGFVTFGSF
                     NSPRKVHSRAVALWARVLAAVPTSRLLFYTEEYGRAGALRIFAEAGVDTNRVEFVGRV
                     SRREYLERHHGCDIALDTFPFSGGTTTLDSAWMGVPTVTLLGSTVLQRAGYAIATHLG
                     LPKLVARSEDEFVERAAALASNLEALSALRAG"
BASE COUNT          412 a          811 c          790 g          451 t
ORIGIN      
        1 cacccatgat gagcgccagg ttcgagcaga gcccgtcgtt cgccccgagc acggcagcgc
       61 gcagggcatt gcccccgact cggcgatgct ggcgctccac gcgcgcgact tctgcccccg
      121 gcgcgccctc cgcgtgctcg acgagaagcg actgaagaac gcgggcgtgg gaccattctt
      181 gcgccaccat cttggtgcct tgcgtttcgg gctgcgctcg gtacacgtct cgatcttgat
      241 attcgcgggt cgcgaccgtc ggcaggaccg tccgcgcacc gaaccgccgc gccacgaacg
      301 aaagcacgcg ggcgcgaaag ctcggcgagg gcggcggcac actggcgttc tccttgcgca
      361 ggcggtcttc ccagaaagct gcgtgcttct cctccatcgc cgcgagcgcc cgataaaggc
      421 gcgcggttcg ctggtcgcgc tctccgtccg ccatcgcgtg gtactgcgcg gcgctgtcga
      481 tctcgtcttg ccgattgcgg cgatatcgct cgatggtacc tggcttcgcg gcgctgctca
      541 cgaccaccat gtaacgcaaa cacggcgcag gctccagcgg ggtggtcatc ctcctcggcg
      601 cggcgtctcg tgcgcacgag cgcctttctt catggtttcc ccgacattgc ttgagaccga
      661 gaggcgtgcg tgcaagtttc gacgtttccg gacggtcacc aagtgaagaa gcaaaagaac
      721 tctcgcgatg tcttcgagca agcgctcgtc ctcgagagtc gcgggcgcgc cgaagaggca
      781 gcggcgaagt accgggcgct catttcgcgc aacgccgagc atgatcgcgc gctctttcgg
      841 ctcgggttga gcctcctagg aaaaggagaa gtcgaggcag cgactcgcta cctcgagcga
      901 gccgtcgagg tccgtcgcga cgaaccgacc tacttgctga gcctcgggga ggcctaccgg
      961 cttcaaggca aagtcgaact agcgggcgcg gtcttgacct atgccgtgaa tatcgacccc
     1021 gacctcacgg aagcccacga gaagctcgcg ccgatccgta tgcatgctgg tgcgtacgag
     1081 gaggcgctct cgcacctgga gaaggttgca gcgctgcgac cagacgatcc tctcgcgcat
     1141 gtcggcctcg cgtgggcgct cttattgctc aatcgcccgg ctcaggcgct cgcgcgcgcc
     1201 gagcgtgcca tcgagctcgc tccgaacctc gcgggcgcgc atcggtgcgc gggggactcg
     1261 ctcgacatgc tcggcgacaa acaacgctcg ctcgagagct atcggcgcgt gctggagctc
     1321 gatccgggag attcggtcgc ccacagcaat ctcatcattt cgatgctgac ggagccaagc
     1381 cacgacgcga aagcagtgct cgcggaggcg cgggcatggg aggcccagca cgccctaccg
     1441 ttgagagccc accagcgcga gcaccaaaat gtcagagatc cggaccggcg cttgcggatt
     1501 ggctacgtct caccggattt ccgggcgcat ccggtccagc agtttctcgt tccgttgctc
     1561 cagcggcacg accccaatgc cgtcgagata ttcctgtatt cgtcggtgga gcgagccgat
     1621 gcacgaacgg aatggtacca gggcttcgct ggggaccgat ttcgcgatat tcggcccctc
     1681 agcgacgtcg aggcggccga gctcgtgcgg cgcgaccaaa tcgatgtctt ggtggacttg
     1741 gcggttcacg gtccgggcaa ccgcctgcgc gtcttcgcgt gcaaaccggc acccatacaa
     1801 atgacctggc taggctacgc gggcacgacc gggctcgcga gcatcgacta ccgcatcacg
     1861 gacccatatc tggatcctct cgacgcggac ctgagcgtct actcggagag ttcgttgcga
     1921 cttccggcaa ccttctggag ctacgacgcg ctggaagtga acctcgtcgt gaaccctgcg
     1981 cctgcggtgg aacggggttt cgtgacgttc ggctcgttca atagtccaag aaaggtgcat
     2041 tcgagagcag tcgcgctctg ggctcgcgtt ctcgctgccg ttcctacttc tcgcctgctc
     2101 ttctacacgg aggagtacgg cagggccggt gctctgcgca tcttcgccga ggcgggcgtg
     2161 gatacgaatc gcgtcgagtt cgtcgggcgc gtttcgcgtc gcgagtacct cgagcggcac
     2221 cacggttgcg acatcgcgct cgatacattc ccgttttcgg ggggcacgac cacattggac
     2281 tcggcctgga tgggagtgcc gaccgtgact ctgctcggct ctacggtgtt gcagcgcgcc
     2341 ggttacgcca ttgccacgca cctcgggcta ccgaagctcg ttgcgcgctc cgaagacgaa
     2401 ttcgtcgaac gagcggctgc gctcgcgagc aacctcgagg cattgagcgc gcttcgcgcc
     2461 gggc
//
LOCUS       DASTKS010001226         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1986017, whole genome shotgun sequence.
ACCESSION   DASTKS010001226 DASTKS010000000
VERSION     DASTKS010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986017"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFQ35_26175"
     CDS             <1..672
                     /locus_tag="VFQ35_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934227.1"
                     /translation="EENDDPTMRITALTALTAPTLLHTLRNTPVVRAQALGTAAFRDL
                     STAQGEAALDAATLEGIFQSVDLTALEAAVAWTSTCIERLSQVDRIFEERTGSRGPDL
                     TPLLRIFRDALNFARPRLENRLPAAADAPGDGAGVTAAQAGGAAPSAPRASLAGEVNS
                     REDVVRAIDKICAYYARFEPSSPLPLMLQRCKKLVPMSFMDILKELAPNGLPQLETVV
                     GKTEE"
     gene            745..1254
                     /gene="tssB"
                     /locus_tag="VFQ35_26180"
     CDS             745..1254
                     /gene="tssB"
                     /locus_tag="VFQ35_26180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HET9934228.1"
                     /translation="MSSQKFIGRNRAPRVQIEYDVELYGAEKKVQLPFVMGVMADLSG
                     NAGKAQPPVADRKFLEFSAENLDDRMKAMEPRVQMQVPNKLTGEGNMSVDLTFQKLED
                     FSPGAVAKHVPALNQLLQARQQLANLITYMDGKTGAEDLIAKILSDKNLLQSLASAPK
                     PAEEGEKKE"
     gene            1259..2755
                     /gene="tssC"
                     /locus_tag="VFQ35_26185"
     CDS             1259..2755
                     /gene="tssC"
                     /locus_tag="VFQ35_26185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET9934229.1"
                     /translation="MSEATEKASSQGQTATLEGDDFTALLRKEFKPQTDRAREEVESA
                     VMTLAREALSSTATTTSDAVASIQAIIAEIDKKLTAQVNEILHNQDFQSLEGSWRGLH
                     YLVNNTETDTMLKIRCMNVSKKELHKTLKRFKGAAWDQSPIFKKLYEEEYGQLGGEPY
                     ASLVLDYYFDHSPTDVETLGEMSKIAAACHAPVLTAAAPTLFQMESWQELANPRDIAK
                     IFSTPEYAAWRSLRESEDARYLGVAMPRFLARRPYGAKSEPVEEFDFEEDASGPNDKK
                     YTWANAAYAMAVNVNRSYKLYGWCSRIRGIESGGAVEGLPTHTFPTDDGGVDMACPTE
                     IGISDRREAELAKAGFMPLVHKKNTDFAAFIGAQSLHKPAIYEDADATANAALAARLP
                     YLFATCRFAHYLKCIVRDKVGSFKGTDDMQKWLQGWITQYVDGAPQHSSEETKARKPL
                     ASAEVTVTPDEANPGYYSSSFFLRPHYQLEGLTVSLRLVSKLPSAKTG"
     gene            2768..>3223
                     /gene="tssE"
                     /locus_tag="VFQ35_26190"
     CDS             2768..>3223
                     /gene="tssE"
                     /locus_tag="VFQ35_26190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HET9934230.1"
                     /translation="MAELTPKERLQPSLLDRLSDDEPDRTRESREKRVLSPQRLRESV
                     RRDLSWLLNNTHLATVQDLDPYPHVEQSTLNYGMPDLAGKTASTVDNAALEKAIRRAI
                     WEFEPRLLKESVRVKAVVNEKQMNHNALCFTIEAELWAQPLPLRLFLRTD"
BASE COUNT          665 a         1051 c          991 g          516 t
ORIGIN      
        1 gaagaaaacg acgacccgac gatgcgcatc acggcgctca ccgccctcac cgcgcccacg
       61 ctcttgcata cgctacgaaa cactcccgtg gtgcgggccc aagcgctcgg cacggctgcg
      121 tttcgcgatt tgagcacggc ccaaggggaa gcggcgctcg atgcggcgac gctcgagggg
      181 atcttccagt cggtcgatct gaccgcgctc gaagcggctg tggcctggac cagcacctgt
      241 atcgaacgac tgagtcaggt ggaccgcatc ttcgaagagc gcaccggcag ccgcggaccg
      301 gatctgactc cccttttgag gatttttcgc gatgccctca acttcgctcg cccgcggctc
      361 gagaaccggc ttcctgctgc tgcggatgcg cctggcgacg gagcaggcgt gactgccgcg
      421 caggcgggcg gcgcagcgcc gagcgcaccg cgcgcatcgc tcgcggggga ggtcaactcg
      481 cgcgaagacg tagttcgagc aatcgacaag atttgcgcgt attacgcgcg tttcgagcct
      541 tcgagcccgc tgccgctgat gctgcagcgc tgcaagaagc tcgtccccat gagcttcatg
      601 gacatcttga aggaacttgc gccaaatggc cttccccagc tcgaaacagt tgtgggcaag
      661 acggaagagt agtgcaagaa ttgcgacacc tgggtcgggc ggggaggctc gtccgtcggc
      721 aatgaccggt atgaggagtg tgtcatgagt agccagaaat tcattggtcg aaaccgcgcg
      781 ccgcgcgtgc agattgagta cgacgtcgag ctttacggcg ccgagaagaa ggtccagctc
      841 ccgttcgtga tgggcgtgat ggcggatctc tccggcaacg caggcaaggc tcaaccgccg
      901 gtcgccgacc gcaagttcct cgagttttcc gccgaaaacc tcgacgatcg catgaaggcg
      961 atggaaccgc gcgtgcaaat gcaggttccg aacaagctga ccggcgaagg caacatgtcg
     1021 gtcgacctca cctttcagaa gctcgaggac ttctcccccg gagcggtcgc gaagcacgtg
     1081 ccggcgctga accagttgct gcaggcacgg cagcagcttg caaacctcat cacctacatg
     1141 gacggcaaga ccggagccga ggatttgatc gcgaagatct tgagtgacaa gaacctgctt
     1201 cagtcgctcg cctccgctcc gaagcccgcc gaagaaggcg agaagaaaga gtaagccaat
     1261 gagcgaagca accgaaaagg cctccagcca gggccaaacc gcaaccctcg agggagacga
     1321 cttcaccgcg ctcctccgca aggagttcaa gccccagacc gaccgtgcgc gcgaagaggt
     1381 cgagtcggcg gtgatgacgc tggcccgcga ggcactatcg tcgaccgcga cgaccacgtc
     1441 cgacgcggtc gcctcgatcc aggccatcat cgccgaaatc gacaagaagc tcaccgcgca
     1501 ggtcaacgag atcctgcaca accaggactt ccagtcgctc gaaggttcgt ggcgaggtct
     1561 gcactacctc gtcaacaaca ccgagaccga cacgatgttg aagatccggt gcatgaacgt
     1621 ctcgaagaag gagctccaca agaccctcaa gcgcttcaag ggcgcggcgt gggatcagag
     1681 ccccatcttc aagaagttgt acgaggagga gtacgggcag ctcggcggcg agccttacgc
     1741 ttcgctcgtg ctcgactact acttcgacca cagccccacc gacgtcgaga cgctcggcga
     1801 aatgtcgaag atcgcagcgg cgtgtcacgc gccggtgctc accgcggccg ccccgaccct
     1861 attccagatg gagtcgtggc aggagctcgc gaacccgcgc gacatcgcga agatcttctc
     1921 gacgcccgag tacgcggcct ggcgctcgct ccgcgagtcc gaagacgcgc gctacctcgg
     1981 cgtggcgatg ccccgcttct tggcgcgccg cccttacggc gcgaagtccg agccggtcga
     2041 ggagttcgac ttcgaggaag acgcgagcgg gccgaacgac aagaagtaca cgtgggcgaa
     2101 cgctgcctac gcgatggccg tcaacgtcaa ccgctcgtac aagctgtacg gctggtgctc
     2161 acgcattcgc ggcatcgagt cgggcggcgc ggtcgaaggc ttgcccacgc acaccttccc
     2221 gacggacgac ggtggcgtcg acatggcttg cccgaccgag atcggcatca gtgatcgccg
     2281 cgaagccgag ctcgccaaag ccggcttcat gccgctcgtg cacaagaaga acaccgactt
     2341 tgcggcgttc atcggcgcgc agtcgctgca caagccggcc atctacgaag acgccgacgc
     2401 gacggcgaac gcggctctcg cggctcgcct gccgtacttg ttcgcgacgt gccgtttcgc
     2461 gcactacttg aagtgcatcg tccgcgacaa ggtcggctcc ttcaaaggca ccgacgacat
     2521 gcagaagtgg ctccagggtt ggatcactca gtacgtcgac ggcgcgcccc agcactcgag
     2581 cgaagagacc aaggcgcgca agccgctcgc ttccgccgag gtgacggtca cgccggacga
     2641 agcgaacccg ggctactact cgtcgagctt cttcttgcgc ccgcactatc agctcgaggg
     2701 gctcaccgtt tcgctccgcc tcgtttccaa actcccgtcc gcgaagacag gataatcgac
     2761 cgcgtaaatg gcggagctta cgccaaaaga acgcctgcag ccgtcgttgc tcgatcgctt
     2821 gagtgacgac gaacccgaca ggacccgcga gtcccgtgaa aagcgggtcc tgtcgccgca
     2881 gcgtttgcgc gagagcgtgc ggcgcgatct ctcgtggctc ttgaacaaca cgcacctcgc
     2941 gacggtgcaa gatctggatc cgtacccgca cgtcgagcag tcgacgctca actacggtat
     3001 gcccgacttg gccgggaaga ccgcgtctac ggtcgacaac gccgcgctcg aaaaggccat
     3061 ccgccgggcc atctgggagt tcgagccgcg actcttgaaa gagtcggtgc gtgtcaaagc
     3121 cgtggtcaac gagaaacaga tgaaccacaa cgcgctctgc ttcacgattg aagccgagct
     3181 ctgggcacag ccgctcccgc tccgcttgtt cttgcgcacc gac
//
LOCUS       DASTKS010001227         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1986130, whole genome shotgun sequence.
ACCESSION   DASTKS010001227 DASTKS010000000
VERSION     DASTKS010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986130"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFQ35_26195"
     CDS             <1..949
                     /locus_tag="VFQ35_26195"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9934231.1"
                     /translation="LSRACSGRVLGGWPGGALLMLYHVTHSTTYEYGEAVSICHNEAR
                     LTPRRTENQLPVATRLVIEPSAVVLAEDRDYFGNIVHFFTLEEAHLRLSVTATSEVDL
                     VPYERPVLSLSPAWESVRDSVRHERSVEGLRALEYTLESPHVRLSPGLQEYAAPSFPK
                     GRPLLECVLELTTRIFTEFKYEPGATSLATPLTEVLQTRRGVCQDFAHLQIGMLRALG
                     IPARYVSGYIHNAPPPGQLRLQGADASHAWLSVWCPGFGYVDFDPTNGTIPCDEHLTL
                     AWGRDFSDTSPLRGVILGGGNHIVRVGVDVLRVAAPNAN"
     gene            complement(1035..1478)
                     /locus_tag="VFQ35_26200"
     CDS             complement(1035..1478)
                     /locus_tag="VFQ35_26200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934232.1"
                     /translation="MMLQVLKSLRDVEGVFGSFALDESGAVLERDLPAVYHDDVILES
                     GPRIVRVCEAIEHGGEALDSLVLRFADYKLHIRRSRGGLLCVIANADSNPPALRMALA
                     LVARRLGTVSPPPKPAKPPPASPEVTSIQTVNPAKQRTYRGLPVR"
     gene            complement(1487..2224)
                     /locus_tag="VFQ35_26205"
     CDS             complement(1487..2224)
                     /locus_tag="VFQ35_26205"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9934233.1"
                     /translation="MEATVPIDLESSSDPRRGFSASLSAASLADLVQLECLSGTTCVA
                     RITASDEVGYLYFRGGRIVHAVSASNVGEAAALEILSWDAGSFEVCNAGWPDSDSIYA
                     PFQSLLMRAAQARDEGARDNLVHFPSTNMKVPQPQKRAASNEHDEPTPASGVTKVRAA
                     ARLDAQGQVVMAKGQASQDLADATALTLRLARLIGESLGLEGLASIEATARDDQLLVL
                     MESDGGALALRAPADADLSALRARYGV"
     gene            complement(2224..2811)
                     /locus_tag="VFQ35_26210"
     CDS             complement(2224..2811)
                     /locus_tag="VFQ35_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934234.1"
                     /translation="MATETASLQSSPARARRGFETTAGEKGALSVALWGDLGLGWFRR
                     FASAMARRGISIKSAAGTRDGDESWSARIELEVENASVDPKSLNYLALTEEGDGDPLE
                     LPPIDRFRIERTETAAICLRVFAKDRVGLLSALLDRMQFLGLFPVRLRVTTDGPVVDD
                     TIWLKGVADHPPSMEAERALVALLQQLSSSSGEPR"
     gene            2976..3755
                     /locus_tag="VFQ35_26215"
     CDS             2976..3755
                     /locus_tag="VFQ35_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934235.1"
                     /translation="MLTKHTPFALLLSLLVAACGGSKPPAEAPSNEGASPDEKADAAT
                     SEKSASSEEKSAPADSSADPGIPKDCFKSSGVCFPHPKFVKKLCNTKNPSMALYLFGN
                     GSKWTHGFLTRKTEAWNASGGASSGGYLEFDEEVLILAERKADTGGMQVSGAGGGYDA
                     LRWDGSCVTLSGEELTQNRPPSPKSPGIEWRFLDDNVQEALRKDSKINSAYLDRRKYC
                     KGAVSGDVSDKCVKADKQLSDSIVAYLRGGGAVPVPTTLPQ"
     gene            3918..>4616
                     /locus_tag="VFQ35_26220"
     CDS             3918..>4616
                     /locus_tag="VFQ35_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934236.1"
                     /translation="MYVDTLRDNMLSGEETTFLNGIPFGTAYRWAHGERAALGNQATT
                     ILSAPVGDYACYEWALKSCQPSNEVLDARAISRGYLTDAFGFRETAGLGPNLCIDDAL
                     AAWGGSDAKKQAVRKLARDVATLIGSVDSAEKLGQVETYLVGKVLVLNGFDVMFEGAK
                     NHAGYKVAWHTNALETESDRPYFPSIEHAWLEYGTTTIQKTSGARIEIGRGQNYGRIG
                     TYETIRMHRIALRSF"
BASE COUNT          844 a         1489 c         1492 g          791 t
ORIGIN      
        1 gctatctcgc gcatgctcag gccgcgttct cggtgggtgg cccggcggag cgctcctaat
       61 gctctatcac gtcactcatt cgactaccta cgagtacgga gaagccgttt cgatctgtca
      121 caacgaggcg cgactcacgc cgcgacgcac cgagaaccag ctgcccgtcg ccactcgtct
      181 cgtgatcgag ccctccgccg tcgttttggc ggaagatcgg gactatttcg gcaacatcgt
      241 gcacttcttc acgctcgaag aggcgcactt gcgactctcg gtgacggcaa cttccgaagt
      301 cgatttggta ccgtacgagc gtcccgtgct gtcgctctca cccgcctggg agtcggtgcg
      361 cgacagcgtg cgccacgaac gcagcgtcga agggctgcgg gcgctcgagt acacgctcga
      421 gtcgccgcac gtgcgccttt cgcccgggct ccaggagtac gcggcgccgt cgttcccgaa
      481 ggggcgcccg ctgctcgagt gcgtgctcga gctcacgacg cgcatcttca ccgagttcaa
      541 gtacgagccc ggcgcgacga gcctcgcgac gccgctgacc gaggtcttac agacacgccg
      601 cggcgtttgc caagacttcg cgcacctgca gatcgggatg ctgcgcgcgc tcgggatccc
      661 ggcgcgctac gtgagcggtt acatccacaa cgcgccgcct cccgggcagc tacggctgca
      721 aggcgccgac gcgtcgcacg cttggctctc ggtgtggtgt cccggctttg gctacgtcga
      781 cttcgacccg acgaatggga cgattccgtg tgacgagcac ctgaccttgg cgtgggggcg
      841 cgacttcagc gatacgagcc cgctgcgcgg agtgatcttg ggtggaggca accacatcgt
      901 gcgcgtcggc gtcgacgtgc tgcgcgtcgc cgctcccaac gcgaactgag gacggggaag
      961 gcccgcgaat gagtcacgcc gacgcggggc gttcacgtcg gcgtggtccg gccccgcctc
     1021 ttgccgtcca agtttcagcg cacgggcagc ccgcgatacg tgcgttgctt ggctgggttc
     1081 acggtttgaa tcgaggtcac ctcggggctc gccggcggcg gcttggcggg tttcggggga
     1141 ggcgaaaccg tgccaagccg ccgcgcgacg agggccagcg ccatgcgcaa cgccgggggg
     1201 ttcgaatcag cgttggcaat gacgcaaagt aggccccccc tcgaccggcg gatatggagt
     1261 ttgtagtccg cgaaacgcag tacgaggcta tcgagcgctt cgccaccgtg ttcgatagcc
     1321 tcgcacacgc gtacgatgcg aggaccactc tccagtatca cgtcatcgtg atacaccgcc
     1381 ggcaaatctc tttcgagcac cgcgcccgat tcgtcgagcg cgaagctccc aaaaactcct
     1441 tccacgtcgc ggagactctt caaaacctgt agcatcatgg gtgagctcac acgccgtacc
     1501 gcgcgcgtag ggccgagagg tcggcgtcag cgggagcgcg cagcgcgagc gcgccaccgt
     1561 cgctctccat caaaacgagg agctggtcgt cgcgcgccgt cgcctctatc gacgccaatc
     1621 cctcgagccc cagtgactca ccgatcagcc gcgcgagccg taacgtcagc gccgtcgcat
     1681 cggccaggtc ctgggacgct tggcccttcg ccatcacgac ctgcccctgc gcatcgagtc
     1741 gggccgcggc gcgtaccttg gttacgcccg aggcgggggt gggctcgtcg tgctcgttcg
     1801 acgccgcgcg cttctgcggc tgtgggacct tcatgttggt gctcggaaaa tggaccaggt
     1861 tgtcccgcgc gccttcgtcg cgagcttgcg ccgctcgcat cagcaagctc tgaaacggcg
     1921 cgtagataga gtcgctatcg ggccagccgg cgttgcacac ttcgaacgag cccgcgtccc
     1981 aactcaagat ctcgagcgcg gccgcctcgc ccacgttcga tgccgagacg gcgtggacga
     2041 tgcgcccgcc acggaagtac aggtacccga cctcgtcact cgcggtgatt cgagcgacgc
     2101 aagtggtccc cgacaaacac tcgagctgaa ccaagtcggc gaggctcgcg gcgctcaggc
     2161 tcgccgagaa gccgcgccgg ggatcgctcg agctctccag gtcgatcggc acggtggctt
     2221 ccatcaacgc ggctctccgc tagaggagga gagctgctgc agaagtgcta cgagggcgcg
     2281 ctcggcttcc atcgagggag gatggtcagc gacgcccttc agccaaatcg tatcgtcgac
     2341 gacggggccg tcggtggtaa cgcgcaagcg caccgggaat agcccgagga actgcatgcg
     2401 gtcgagcagc gcggacagca ggcccacccg atctttcgcg aagacgcgaa ggcaaatcgc
     2461 ggccgtctcg gtgcgctcga tgcgaaagcg atcgatcggc gggagctcga gagggtcgcc
     2521 gtcgccctcc tcggtgagag caaggtagtt cagcgacttc ggatcgacac tcgcgttttc
     2581 gacttcgagc tcgattctgg cgctccagct ctcgtcgccg tcgcgtgtgc ctgccgcgct
     2641 cttgatgctg atgccacggc gagccatcgc gctcgcgaag cgtcgaaacc aaccaagccc
     2701 gagatcaccc cagagcgcga cgctgagtgc gcccttctcg ccagcggtcg tctcgaaacc
     2761 gcgacgcgcc cgcgcgggtg agctctgaag agaagctgtc tcggtggcca tttttggcag
     2821 agtaggctcc accctcggat tcacggctgt ttcggtcgca ttgccagccg tgtgaacgcg
     2881 gaaaaattcg gatgtcatcg ttcgaagccg cgtcttcttg gcggcacgcg gcgaacgctg
     2941 gtccttcgcg cgcctttgat ttaaaaccgc gcgacatgct cacgaaacac acgccgttcg
     3001 cgctcctcct gtcgttactc gtggccgctt gtggcggtag caagccgcca gcagaggctc
     3061 cttcgaacga gggcgcgagc cccgacgaaa aggccgacgc ggcgacgagc gagaagtcgg
     3121 cgtcgtccga ggagaagagc gcacccgccg actcttccgc agatcctggc attccgaaag
     3181 attgcttcaa gtcgagcggc gtatgctttc cgcacccgaa attcgtgaag aagctgtgta
     3241 acaccaagaa cccctcgatg gcgctctacc tcttcggcaa cggctcgaag tggacgcatg
     3301 gcttcctcac acgaaagaca gaggcgtgga atgcttcggg cggcgcttcg tcgggcggct
     3361 acttggaatt cgacgaagaa gttttgatcc tcgcggagcg caaggcggac acgggcggca
     3421 tgcaagtgag cggcgcgggt ggcggctacg acgcactccg ctgggacggc tcgtgcgtga
     3481 cattgtctgg cgaggagctc acgcaaaacc gcccgccatc gccgaagtca ccgggcatcg
     3541 agtggcgctt cctcgacgac aacgtccaag aggcactgcg aaaagactcg aagatcaaca
     3601 gcgcatacct cgaccgccgc aagtactgca aaggcgccgt ctcgggtgac gtcagtgaca
     3661 agtgcgtcaa agccgacaag caactcagcg attccatcgt cgcctatctg cgcggcggcg
     3721 gtgcggtgcc ggtgccgacg acgcttccgc agtgaggctc gtctctcgag gcttcgaagc
     3781 gtcgtgttcg tgacgcgagc gccgtgacgg agcggcgcaa accgccgccc gtcgaacgcg
     3841 gttgccgtac ttcgccaggg cgagcgagcg caacggcctc cgggcggtga tgcgctcgcg
     3901 aaagttgtgt gtccggaatg tacgttgata cgttgcgaga caatatgctc agcggcgaag
     3961 agacgacttt cctcaatggc atcccgttcg gaaccgcgta caggtgggcg cacggcgagc
     4021 gagcggctct cggcaatcaa gccacgacga tcctgtcggc gccggtcggt gactatgcgt
     4081 gttacgagtg ggcgctcaag agctgccaac cctccaacga ggtcctcgac gctcgagcca
     4141 tatccagagg ttacctgacc gatgcctttg gcttcagaga gacggcgggg ctcggcccca
     4201 atctttgcat cgacgatgct ctcgccgcat ggggcgggag cgacgccaag aagcaggcgg
     4261 taaggaagct cgcgcgggac gtcgccacgc tcatcggaag cgtcgatagt gcagagaaac
     4321 tcggccaggt agagacctat ctcgtcggca aagtgttggt gctcaacggg ttcgacgtca
     4381 tgttcgaagg ggcgaagaac cacgctggtt acaaggtggc gtggcatacc aacgcgctcg
     4441 aaaccgagag cgatcgaccg tacttcccgt cgatcgagca cgcttggctg gaatacggaa
     4501 cgaccacgat tcagaagacg agcggcgcgc gcatcgagat cgggcgggga caaaattacg
     4561 ggcggatcgg cacgtacgaa accatccgca tgcatcgcat cgcgttgcgc tcgttc
//
LOCUS       DASTKS010001228         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1090939, whole genome shotgun sequence.
ACCESSION   DASTKS010001228 DASTKS010000000
VERSION     DASTKS010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090939"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /gene="fliD"
                     /locus_tag="VFQ35_26225"
     CDS             <1..339
                     /gene="fliD"
                     /locus_tag="VFQ35_26225"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HET9934237.1"
                     /translation="TLSLDSTKLTQAISRDSASVSNVLAGPASGKGVMDVMSSLADGI
                     TTDNGILTLRSDSLSKNSKQIATRLTAEQDRLNREADNLRKQFTAMDTTVAGNNAQLK
                     YLQNLYTSSK"
     gene            373..771
                     /gene="fliS"
                     /locus_tag="VFQ35_26230"
     CDS             373..771
                     /gene="fliS"
                     /locus_tag="VFQ35_26230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HET9934238.1"
                     /translation="MFETAAARYTQIRNTTATPGEVLLALYEGLFRFLNGARLCIEKK
                     NLVPAREQLSKAYAIVSELYIALDYTAAPELCSQLEGVYGFAMDRLMHASRKCEIGPI
                     DEVIRVMTPLREAWKIAVPQAQREQAANNR"
     gene            775..2097
                     /locus_tag="VFQ35_26235"
     CDS             775..2097
                     /locus_tag="VFQ35_26235"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9934239.1"
                     /translation="MSESVPETKQPSRTQAPRYSPTPKRGVFQASFADGTVLELEPGR
                     SRAYPGLRGQAPSMLAFYDAFADFVGVGRFLDAGSGVGLGALRLGQRGLHVIAVDEDL
                     TAIEFARRHCPDAEHQHKNLAALELASPADGAIIADVLGMCEDPEPALAGVARALRLG
                     APLLVAEPAAHVSQRLSVPQRRGFSVPRLRSLLVRTGFEVEAVIADRVPFVALLARAA
                     FPQVRAAFAEAYAAAASGELDRAVNELNRARGTGRFRVEAEILLAEAELRLVSGDGDR
                     AANAFFRTRELDAEDPRPLVGLGRIALASGNHGDALELALDALRCDPTDPTAYVLVAL
                     ASSALGHADSFSAWRAAARLAPDDSFVVGELTRAALARGDHPVALGGLDQLERYGVPL
                     EAAHHVTRAWLLLALGRRNEATIEARIARAKQSTHPELGELERALAER"
     gene            complement(2260..>3035)
                     /locus_tag="VFQ35_26240"
     CDS             complement(2260..>3035)
                     /locus_tag="VFQ35_26240"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9934240.1"
                     /translation="TLLRNPDEPRSKVVSLLEQDPALCGRLLRVANSALYAPKRECVS
                     VSDAIMRLGNKNVSDIISGIAVFGMFSDVDGLGLRVRDHSAKVASLARVLAIEWCREG
                     AEHVFLAGLMHDVGKLMMMQAGTPRYDLIDPKLLAEPDRIHLSERELTGFDHATLGAH
                     VLREWQFPNDVARVVAWHHQPGRAFAAGAEVGLPVALIRLADRIEVLAGSGEAPDEAF
                     CASLAGDESAAYTNFTAKELRTLWQKLRTACRETLGALR"
BASE COUNT          512 a         1064 c          971 g          488 t
ORIGIN      
        1 actctcagcc tcgactcgac caagctgact caggccatct cgcgagattc agccagcgtg
       61 agcaacgtgc tcgctggccc cgcgagcggc aagggcgtca tggacgtcat gtcgagcctc
      121 gccgacggca tcacgaccga caacggtatc ctcacgctgc gctccgactc gctcagcaag
      181 aactcgaagc agatcgccac tcgcctcacc gccgaacaag accgcctcaa ccgcgaggcg
      241 gacaacctgc gcaaacagtt caccgcgatg gacacgaccg tcgccggcaa caacgcgcaa
      301 ttgaagtacc tccagaacct ctacacctcg tccaagtaac tctcacccat aggaacctag
      361 gaccggactc ccatgttcga gacagcagcc gcacgctaca cccaaatccg caacaccacc
      421 gccaccccgg gtgaggtgct gctggcgctg tacgaaggcc tgttccgctt tttgaacggc
      481 gcgcgccttt gcatcgagaa gaagaacctc gtgcccgcgc gcgaacagct ctcgaaggcg
      541 tacgcgatcg tttcggagct ctacatcgca ctcgactata cagcggcccc cgagctgtgc
      601 agtcagctcg aaggtgtgta cggcttcgcg atggaccggt tgatgcatgc ttcgcggaag
      661 tgtgagattg gtcccatcga cgaggtgatt cgcgtgatga ccccgctgcg cgaagcctgg
      721 aagatcgccg tgccgcaagc acagcgagag caagcggcga acaaccggta aaccatgtcc
      781 gagagcgtcc ccgaaacgaa gcagccgtcg cgcacacagg ctccgcgtta ctccccgact
      841 cccaaacgcg gcgtttttca agccagtttt gccgacggca ccgtgctcga gctcgagccg
      901 ggacgcagcc gtgcgtatcc ggggctgcgc ggtcaggcgc cttcgatgct ggcgttctac
      961 gacgcattcg ccgacttcgt aggcgtagga cgcttcctcg acgcaggctc gggcgtcggg
     1021 ctcggcgccc ttcggctcgg gcagcgtgga cttcatgtca tcgccgtcga cgaagatttg
     1081 acggcgatcg agttcgcccg ccgtcactgc cccgacgcgg agcatcagca caagaacttg
     1141 gctgcgctcg agctggcgag ccccgccgat ggcgccatca tcgccgacgt cctcgggatg
     1201 tgcgaggacc cggagcccgc tctcgccggc gtcgctcgcg cgctccgcct gggcgctccg
     1261 ctgctggtcg ccgagcccgc ggcgcacgtc tcacaacgcc tcagcgtgcc gcagcgccgt
     1321 ggcttttccg ttccgcgctt gcgttcgctg ctcgtgagga ccggcttcga agtcgaggcc
     1381 gtgatcgccg atcgagtgcc gttcgttgcc ctgctcgcgc gcgcggcgtt cccccaggtt
     1441 cgcgccgcat tcgccgaggc gtacgcggcc gcggcatcgg gcgagctcga ccgtgccgtg
     1501 aacgaactga accgtgcgcg cggcaccggt cgcttccgag tggaggcaga aatcctactc
     1561 gccgaggcgg agctgcgcct cgtgagcggc gacggcgacc gcgccgccaa cgctttcttc
     1621 cggactcgcg agctcgacgc ggaagatcca cgtccgctcg tcgggctcgg gcgcatcgcg
     1681 ctcgccagcg gcaatcacgg cgacgccctg gagcttgcgc tcgacgcgct gcgctgtgac
     1741 ccgaccgatc cgaccgctta cgtcttggtg gcgctcgcga gctccgcgct cgggcacgct
     1801 gattcttttt ccgcatggcg cgccgcggct cggctcgccc ccgacgattc cttcgtggtc
     1861 ggtgagctca cccgcgccgc tctcgcgcgg ggcgaccacc ccgtagcgct cggaggcctc
     1921 gaccagctcg agcgctacgg cgtaccgctc gaggccgcgc atcacgtgac gcgagcgtgg
     1981 ctcctgctcg cgcttggccg acgcaacgaa gcgaccatcg aagcccgcat cgcgcgcgcc
     2041 aagcaatcga cgcacccaga gctcggcgag ctcgaacgcg ccctcgccga gcgctagcga
     2101 gcgcgccgct gaacctcaga gcccaatccg cgaactgagt ggcttcggct cgagtcgcgc
     2161 acgcgagcgc tgaccaccgg ctttggtttg ccaaacccat gcgtgggttc ggagatctga
     2221 tggagcggcg ggcgtgggta tcacgaccgc tcgtcgcgac tagcgcaggg cgccgagcgt
     2281 ttcgcggcac gcggtccgga gcttctgcca caaggtacgg agctccttgg ccgtgaagtt
     2341 cgtgtacgcg gccgattcgt cgccggcgag agaggcgcaa aaggcctcgt ccggtgcctc
     2401 gccgctaccg gcaaggacct cgatgcgatc ggcgagccga atcaaagcga ccggcaaccc
     2461 aacctcggcg ccggcggcaa acgcccgccc gggctggtga tgccaggcga cgacgcgcgc
     2521 gacgtcgttg gggaattgcc actcacggag cacgtgggca ccgagcgttg cgtgatcgaa
     2581 gccggtgagc tcgcgctcgc tcaggtgaat tcgatccggc tcggcgagca gcttcgggtc
     2641 gatgaggtcg taacgcggcg tgcccgcctg catcatcatc agcttgccga cgtcgtgcat
     2701 gaggccggcc aggaagacgt gctcggcgcc ttcgcgacac cactcgatcg cgagcacacg
     2761 cgcgaggctc gcgacttttg ccgagtggtc gcgcaccctc aggccgaggc cgtccacgtc
     2821 cgaaaacatt ccgaacacgg cgatgcccga gatgatgtcg gagacgttct tgttgccgag
     2881 acgcatgatc gcgtccgaga cgctcacgca ctctcgcttg ggggcgtaca gcgcggagtt
     2941 tgcaacccgc agtaggcgcc cgcagagcgc cgggtcttgc tcgagcagcg agacgacctt
     3001 ggagcgcggt tcgtccgggt tcctgagcag ggtca
//
LOCUS       DASTKS010001229         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1539140, whole genome shotgun sequence.
ACCESSION   DASTKS010001229 DASTKS010000000
VERSION     DASTKS010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539140"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..>2853
                     /gene="gyrA"
                     /locus_tag="VFQ35_26245"
     CDS             573..>2853
                     /gene="gyrA"
                     /locus_tag="VFQ35_26245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9934241.1"
                     /translation="MEDQPTPPQSPNEHPVSIQDEMRTSYLDYAMSVIIGRAIPDVRD
                     GLKPVHRRILYAMRELNLTPGTSYRKCATVVGQVLGKYHPHGDASVYDALVRMAQPFS
                     MRHMLVDGQGNFGSVDGDPAAAYRYTESRLAKISTDLLTDIEKDTVDFTPNFDGSDSE
                     PVVLPARFPNLLVNGSGGIAVGMATNIPPHNLIEVLDATIHLIRNPSAEIIDLMRFVK
                     GPDFPTAAQIYGKSGIAQAYLTGRGSIIMRGRSEIEKVSGSPEREQIVFTELPYQVNK
                     ARLHAKIGELMRDKRIEGIREARDESDRDGMRLVLELKKDVFPQVVLNQLYRLTDIQT
                     SFGIINLAIVNGRPAVLNLKETLAYFVEHRRDVVTRRTRFELAQAEGQREIVEGLGMA
                     VTEVDVVIRTIRESKDPDEAKERLMALPLHGLEAFVTRAGRPPAEIEEAKARGEYHLS
                     ERQAKAILEMRLARLTGLEQEKLAGEFGELCELIGRLRAILADEVLLLDLIVKELEEV
                     KEKHGEERRTEIVESEAEIQLEDLIQEEDMVVTISHSGYIKRTPVSTYKAQRRGGKGN
                     KGMEAADDDFVNQLFIASTHSFVFFFSNRGKVYVKKVYEVPQAARQAKGRAIINFIEL
                     EEGEKIAAITPVRGFEPGLFITTITRGGQIKKTEVTEYENYRDKGIIGVKIAEDDQLL
                     TACVTDGNAEFLIATRHGQSIRFDEQQVRPMGRGAGGVKAIELEDEDQVVGLVPTEPE
                     RTQVLAVCERGFGKRTPLEE"
BASE COUNT          575 a          944 c          893 g          441 t
ORIGIN      
        1 ttgctcggcg cagcgtcggc cgtcttagga ccgagttgct gtcgtccgca cgcaggcgag
       61 ctgagcgcgc cagattcgtc acgtctcgct agagaccctt cgcacccggc atggaggctc
      121 tccagggcgg ttgcgaaaag agagggcggt tgcgaaaaga gttcgtcgtg atgctttggt
      181 ttgcgtcggg ttcggggccc cagaaccccg aagacggcga gctcagccgg gctgggagat
      241 ccgatgaacg gtgaggcaga cggcccgcgc cgcggttggt cgggagcgcg tccaaaagcg
      301 ctcggttcag cagaaaaacc gccccttcgc gcgactcgaa agctggtcct ccggggttgt
      361 cgcggggcgc cgattagact actgcttcat ccggaagcaa cgagcgaaag ttcaggaaaa
      421 gggcttggtc tgggcgcctc tcatccagcc cggcacacgc ctgatcaccc gagaccttcg
      481 gtgcttccga ggcacgaccc actccgaatc gcgtttctgc cacgcgactc cccagcgtag
      541 aaaacgcatt ccgaacgagc gcattctgag cgatggaaga ccaaccgacg cccccgcagt
      601 cgccgaacga acatcccgtc agcatccaag acgagatgcg cacgagctac ctcgattacg
      661 cgatgagcgt gatcatcggt cgcgccatcc ccgacgtgcg cgacggcttg aagcccgtgc
      721 accggcgcat tctgtacgcg atgcgcgagc tgaacctgac gcccggcacc tcgtaccgta
      781 agtgtgcgac cgtggtcggg caggtgctcg gcaagtacca cccgcacggc gacgccagcg
      841 tttacgacgc tctcgtgcgc atggctcagc cgttttcaat gcggcacatg ctcgtggacg
      901 gccagggcaa tttcggctcc gtcgacggtg acccggccgc cgcataccgt tacacggagt
      961 cccgcctcgc caagatctcg accgacctcc tcaccgacat cgaaaaggat acggtcgact
     1021 tcaccccgaa cttcgacggc tccgacagcg agcccgtcgt tctccccgcg cgttttccga
     1081 acctgctcgt caacggttcg ggcggcatcg cggtcggcat ggcgaccaac attccgccgc
     1141 acaacttgat cgaggtgctc gacgcgacga tccacctcat ccgcaatccc agcgccgaaa
     1201 tcatcgactt gatgcgtttc gtgaaggggc cggacttccc taccgccgcg caaatttacg
     1261 gaaagagcgg catcgcccag gcgtacctca cgggccgcgg cagcatcatc atgcgcggcc
     1321 gcagtgagat cgagaaggtg agcggcagcc ccgagcgcga gcagatcgtt ttcacggagc
     1381 tcccttatca ggtcaacaag gcccgcctcc acgcgaagat cggcgagctc atgcgcgaca
     1441 agcgcatcga aggcatccgc gaagcgcgcg acgagagcga ccgcgacggc atgcgcctcg
     1501 tgctcgagct caagaaagac gtcttcccgc aggtggtcct gaaccagctc taccgcctca
     1561 ccgacatcca gacttcgttc ggcatcatca acctggcgat cgtcaacggt cgccccgcgg
     1621 tgctgaacct gaaagagacg ctcgcttact tcgtcgagca ccgccgcgac gtcgtcacgc
     1681 gccgcacccg cttcgagctc gcgcaggccg aagggcagcg cgaaatcgtc gaaggcttgg
     1741 gcatggcggt caccgaggtc gacgtcgtga tccgcaccat tcgcgaatcg aaggatccgg
     1801 acgaggccaa ggagcgcttg atggcgctgc ccctgcacgg cctcgaggcc ttcgtcacgc
     1861 gcgcgggcag gccccccgcc gaaatcgaag aggcgaaggc gcgcggcgag tatcacctct
     1921 cggagcgcca agcgaaggcc atcctcgaga tgcgcctcgc gcggttgacc ggcctcgagc
     1981 aggagaagct cgccggcgag ttcggcgagc tctgcgagct catcgggcgg ctccgcgcga
     2041 tcctcgccga cgaagtcctc ttgctcgacc tcatcgtgaa ggagctcgaa gaggtcaaag
     2101 agaagcacgg cgaggagcgc cgcaccgaga tcgtcgagag cgaagcggag attcagctcg
     2161 aggacctgat ccaagaagag gacatggtcg tcacgatctc gcactccggc tacatcaagc
     2221 ggacgccggt ctcgacgtac aaggcccagc gccgcggcgg caagggcaac aaggggatgg
     2281 aggccgccga cgacgacttc gtcaatcagc tgttcatcgc ctcgacccac agcttcgtct
     2341 tcttcttttc gaaccgcggc aaggtctacg tcaagaaggt ctacgaagtg ccgcaagccg
     2401 cgcgccaagc aaaaggtcgc gccatcatca acttcatcga gctcgaagag ggcgagaaga
     2461 tcgcggcgat cacgccggtg cgcggtttcg agccgggcct cttcatcacc acgatcacgc
     2521 gcggcggtca gatcaagaag accgaggtca ccgagtacga gaactaccgc gacaagggca
     2581 tcatcggcgt caagatcgcc gaagacgacc agctgctcac cgcgtgcgtg accgacggca
     2641 acgcagagtt cttgatcgcg acgcggcacg gacagtcgat ccgcttcgac gaacagcagg
     2701 tgcggccgat gggtcgcggt gccggcggcg tgaaggcgat cgagctcgag gacgaggacc
     2761 aagtcgtcgg actcgtgccg accgagcccg agcgcaccca ggtgctcgcg gtgtgcgagc
     2821 gcggcttcgg caagcgcact ccgctcgaag agt
//
LOCUS       DASTKS010001230         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_419754, whole genome shotgun sequence.
ACCESSION   DASTKS010001230 DASTKS010000000
VERSION     DASTKS010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419754"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VFQ35_26250"
     CDS             complement(<1..1029)
                     /locus_tag="VFQ35_26250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934242.1"
                     /translation="MRKRAATTRQRRALLLIGALSSGFLILWLSLKSSGADWYISETR
                     ADILYTGLHSFHEFPYFSFALNGGSYLLQDPEGPLLSPTALAIAALGPTLGLRVVTGL
                     WAALGVLGFVAWLRGRVRLEAAMLGGVASVTSLAVLWKVAVGNDMFLWGLALPGILFL
                     VRAVVSRRTIASAIALGVAAGVLLLGPTFLVLTYLFAPVVPLALVYELVTSRVERRDW
                     WRLAALLLLSGVVACCVASPKLALWTVLPMQRPTIDHGVLSLSRSLRGLFNYGATKSS
                     LLHMVSYRDGAPVIGAWDGSEAAMAPSPVATALALLGTVACFRKSPRRALGGMAAALV
                     AIGLTLSSS"
     gene            1246..2301
                     /locus_tag="VFQ35_26255"
     CDS             1246..2301
                     /locus_tag="VFQ35_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9934243.1"
                     /translation="MARRRQVPAGSLPHGEASSSTGLRLHASGDRLSALLAERERLLV
                     QVRKRRAALQAAAERAESAARDMTTHVAPLKAKFDRAREEIAALFDELLAPGRLAPRT
                     RKKVLGLHRSLVAQGLLEPREDETNDDPSESQDPFGDYVEIGAPVYFDEEPEVRVPPA
                     AQHGQSAGRETLRALFRRLALALHPDRTQREDDRQRRTEAMKEATRAYEDGDLARLLE
                     LEKAWQHGSVAPASANDEGRRCEELERTIHELTLQSRELNRELRDLKRYMREELLDVS
                     PQTVVALAKDELTELETIRDFVKAFRDGKITLSQFLEGPRPAGADMSPLEALVYDMVE
                     EETRAGRPRSKSRRRRA"
     gene            2445..2921
                     /locus_tag="VFQ35_26260"
     CDS             2445..2921
                     /locus_tag="VFQ35_26260"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET9934244.1"
                     /translation="MTSGQGPIGPAPSVQSTPSGENPHFAAIGAEGVARLVERFYFHM
                     DSLPDAAGIRALHPADLAPVREVLTRYLTEWLGGPPLYSTERGHPRLRRRHLRFRIGA
                     AERDAWMLCMRAALGEVIGDAKLRAELDAAFFKVADFLRNDAEHVHVHHATTPPTE"
     gene            2925..>3304
                     /locus_tag="VFQ35_26265"
     CDS             2925..>3304
                     /locus_tag="VFQ35_26265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HET9934245.1"
                     /translation="MARIVFFEKVGCANNSRQKAWLTAAGHVVEARDLLHHPWSREEL
                     LSYFAVLPVADWFNRAAPKVKSGEIVPEALDREAALGLLQREPLLIRRPLLYVDDQRA
                     VGFDVERIHAWLGLPEDVVASFRER"
BASE COUNT          622 a         1081 c         1091 g          510 t
ORIGIN      
        1 ggagctcgag agcgtgaggc caatcgcgac gagcgcggcc gccatacctc cgagggctcg
       61 ccgaggcgac ttccggaagc aggccaccgt tcccaaaagc gccaaggcag ttgcgaccgg
      121 cgaaggcgcc atcgcggcct cggagccatc ccaggctccg atgacgggcg ctccgtctcg
      181 ataggagacc atgtgcagta gcgacgactt ggtcgcgccg taattgaaga gcccgcgaag
      241 cgaccgactc aacgagagta cgccgtgatc gatcgtcggt cgctgcatcg gcaggaccgt
      301 ccacagtgcg agcttcgggc ttgcgacgca gcaagcgacg acgcccgaga gcagcaagag
      361 cgccgcgagc ctccaccaat cgcgtcgctc cactcgtgaa gtcacgagct cgtaaaccag
      421 cgcaaggggc actacgggcg caaacaagta ggtcagcacg agaaacgtgg ggcccagcaa
      481 caggacaccc gcggcgacgc ccagcgcgat cgcgctcgcg atcgttcttc gactgacgac
      541 ggcacgaacc aggaacaata tgccgggcag cgccaagccc caaaggaaca tgtcgttgcc
      601 gaccgcgacc ttccacagca cagccaggct cgtaacgctc gcaactccac cgagcatggc
      661 cgcctcgagc cgaactcttc ctcgcaacca cgcgacgaag ccgaggacgc cgagtgccgc
      721 ccacaaaccc gtgaccaccc gcagtccgag cgtcggcccg agcgcggcga tggcgagcgc
      781 ggttggtgaa aggagcggcc cttccggatc ttgcagaagg taagagcccc cgttcagcgc
      841 gaacgaaaag taggggaact cgtgaaagct atgtaacccc gtgtaaagaa tgtcggcccg
      901 ggtctcggag atgtaccaat ctgcgccgct gctcttcagg ctcagccata agatcagaaa
      961 gcccgacgag agcgcaccga tgagcagtaa ggcgcgccgt tgacgggtag ttgcggcgcg
     1021 cttacgcatc gaagtagtcc gtgtgcgggc ggttcgttgc gctcggagtt acggttgggc
     1081 accgagcatt cgatggcgag ggacggtttg ggcgccgcac gatgaaggcg tctgagccta
     1141 gtcgcagtcc cacgacgagt cgatcccgaa ccggtgggcg aactttcgtg cggcgggcgc
     1201 gattttcggc tcgccgcggc ggcgcggttg aacgatatcc gggttatggc tcgtcgtcgt
     1261 caggtccccg ctgggtcgtt gccgcacggc gaggcttctt cctcgacagg gctgcgcctg
     1321 catgcgtcgg gcgaccgtct gagtgcgctg cttgctgaac gagagcggct gctcgtccag
     1381 gtacgcaagc gaagggcagc gttgcaggct gccgccgagc gcgccgagtc ggccgcgcgc
     1441 gacatgacaa cgcacgtggc gccgctgaag gccaagttcg accgagcacg cgaggagatc
     1501 gctgcgctct tcgatgagct cctcgcgccg ggtcggctcg cgccgcgaac ccgaaagaag
     1561 gtcctggggc ttcatcgctc gctcgtcgct caggggcttc tcgagccacg cgaggacgaa
     1621 acgaacgacg acccctccga gtcccaggac cccttcgggg actacgtcga gatcggcgcg
     1681 cccgtttact tcgacgagga gcccgaggtg cgggtaccgc ctgcagcgca gcacgggcag
     1741 tccgcagggc gcgagacgct tcgcgctctg ttcaggcgct tggcgttggc gctgcatccg
     1801 gatcgaacac agcgcgaaga cgaccgccag cgtcgcaccg aagcgatgaa agaggcgacg
     1861 cgcgcctacg aagacggcga cctcgcgcgt ctgctagagc tcgagaaggc ttggcaacac
     1921 ggctcggtcg ctcccgccag cgcgaacgac gaaggtcgtc ggtgcgagga gctcgaacga
     1981 acgattcacg agctgacttt gcagagccgc gagctcaacc gcgagctccg cgacctcaaa
     2041 cgatacatgc gcgaggagtt gctcgacgtc tcaccacaaa ccgtcgtcgc gctggccaag
     2101 gacgagctca ccgagctcga gacgatccgc gatttcgtga aagcgttcag ggacggcaag
     2161 atcacgctgt cgcagttcct cgaaggccca cgcccggctg gcgccgacat gtcaccgctc
     2221 gaagcactgg tctacgacat ggtcgaagag gagacgcgag ccggtcgtcc ccgctcgaag
     2281 agtcgtcggc gccgagcgtg agggcctgag tctcgggcac gagtcgccct ggcggccgag
     2341 ccgtgtgttg ggattgtcgg aaacacgaca gcgcgatggc tcgctcaagt atttgaaatc
     2401 actagatctc ctcgcaggca cgcgcgttgc tgtaagcgcg cgacatgacg agcgggcaag
     2461 gtccgattgg cccagcaccc tccgtgcaat ccacgccttc gggcgaaaac ccgcacttcg
     2521 cagccatcgg tgcagaaggc gttgcgcggc tcgtcgagcg cttctacttc cacatggact
     2581 ccctgccaga tgcggccggg atccgcgcgc ttcacccagc cgacctcgcg cccgtgcgcg
     2641 aagtgctgac gcgctacctg accgagtggt tgggcgggcc gccactttac tcgactgagc
     2701 gcggacaccc acgcctgcgc cggcgtcatc tgcgttttcg gatcggcgct gcggagcgcg
     2761 atgcgtggat gctgtgcatg cgcgcggcgc tcggcgaagt gatcggcgac gcgaagctgc
     2821 gcgccgagct cgacgcggct ttcttcaagg tggcggactt tctccgcaat gacgcggagc
     2881 acgtgcacgt ccaccacgcc accaccccgc cgaccgagtg agccgtggcg cgcatcgtgt
     2941 tcttcgagaa ggtcggctgc gcgaacaaca gccgccaaaa agcttggctc accgcggcag
     3001 gccacgtcgt cgaagcgcgc gacctgttgc accacccgtg gtcgcgcgaa gagctgctga
     3061 gctacttcgc ggtgttgccc gtcgccgact ggttcaatcg cgccgcgccg aaagtgaagt
     3121 cgggtgaaat cgtgccggaa gcgctcgatc gcgaggcggc gctcggcctg ctccagcgtg
     3181 agccgctgct aatccgtcga ccactgctct acgtcgatga ccagcgcgcg gtcgggttcg
     3241 atgtcgagcg cattcacgcc tggctcggct tgcccgaaga tgtggttgcg tcatttcgtg
     3301 aacg
//
LOCUS       DASTKS010001231         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_419755, whole genome shotgun sequence.
ACCESSION   DASTKS010001231 DASTKS010000000
VERSION     DASTKS010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419755"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..827)
                     /locus_tag="VFQ35_26270"
     CDS             complement(21..827)
                     /locus_tag="VFQ35_26270"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9934246.1"
                     /translation="MTCRSVVPLLEPFVDGELLPEKVVEVEQHLEECSRCVERVRLGR
                     AVRISLRRAVRPSSEPSTAFRARLAAAMQATHEREQMLEQQIERTRMLSWRTIVPVAV
                     AAAAMLVVANVDKNPVRTASGQIESANVDQLIEELINHHVQGRPEVTSLSLLPSLEKE
                     VGVPVQPPLLTQYGGRWEGASVVPMNNQRAASLRYKVAGHPVTLYMYDSSRFQQLENR
                     LQRRLVGDAPVYVGARRGYSIGATEHRGVGYAVATDLNEPETAELVAALH"
     gene            complement(824..1477)
                     /locus_tag="VFQ35_26275"
     CDS             complement(824..1477)
                     /locus_tag="VFQ35_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9934247.1"
                     /translation="MASRALSNQQFEQEVLLHLPSLLSVASRLMKNGPEAEDLVQDAV
                     LKAIRARGQFETGTNLRAWLLKILTNTFINRYRRGGLERSVLDGPDADPLADGWVSAS
                     TMESLRDPESQALRPILEAEIGKALDELPTEFRLAVVLSDVEDLSYKEISDIMGCPIG
                     TVMSRLHRGRRMLKSRLYEHARAIGLVGPEATVGELPKKEEPVVEMEAYRRKKGARA"
     gene            complement(1513..2124)
                     /locus_tag="VFQ35_26280"
     CDS             complement(1513..2124)
                     /locus_tag="VFQ35_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HET9934248.1"
                     /translation="MNGDAGSESEAPPSQRRIIKRYSNRKLYDTKGSSYVTLLQIAQM
                     IRDGEDVQIIDNATKEDKTDVTLALIISEELREKPRAIPLTTLKALIRHRGERILTQL
                     REGPIGKLIPREEGEIAGAPMEPPVPGEEDMSQGKENKGLRATLEQWQSAIDERIRAV
                     LPNFAAFRDLETEVKRLSERLDSIERRLGDRSSEKPPEAPGNS"
     gene            2516..>3354
                     /locus_tag="VFQ35_26285"
     CDS             2516..>3354
                     /locus_tag="VFQ35_26285"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934249.1"
                     /translation="MRALSVAFLALFFGLSALPLAARGLKIAEAKLFGVTTLIEPVDL
                     SWPAYRDGKFQESFEASFDERLGFRTHAIRADNELNLRVFRQLGYDPHTGRVLGKDDY
                     IYDRVYVDSFNRRDDVPIEAIEPRVKSMLALQRYMESRGKTLLVLITPSKAVLYPEYL
                     PRRMIDREQQRGPSNYHKLLILLQRYGVHYFDAQGYLAALKGKVPFTLFPNSGIHWGD
                     PAACEVSARVLERIGEQLQRRVPRLQCEPYRPSADLQRDDTDVLDVCNLWSPERLVRP
                     GFYA"
BASE COUNT          546 a         1104 c         1039 g          665 t
ORIGIN      
        1 aatttgggac accccctagt tcagtgcagc gcggcgacga gctctgccgt ctcgggctcg
       61 ttcagatccg tcgcgacggc gtaaccgacg ccgcggtgct cggtcgcacc gatcgagtag
      121 ccgcggcgcg cgccgacgta cactggcgca tcgccgacca gccttcgctg caggcggttc
      181 tcgagctgct gaaagcgcga cgagtcgtac atgtacaggg tgaccgggtg ccccgcgacc
      241 ttgtagcgga gcgaggccgc gcgctggttg ttcatcggaa cgacgctcgc gccttcccaa
      301 cggccaccgt attgcgtgag aagcggcggc tgaacgggaa cgccgacctc tttctcgagc
      361 gacggcaaga gcgagagcga ggtcacctcg ggacgaccct ggacgtgatg attgatgagc
      421 tcttcgatca actgatcgac gttggcagac tcgatctgtc cgctcgcggt gcgaaccggg
      481 ttcttgtcga cgttcgcaac gaccagcatg gcagccgcag ctacggcgac tgggacgatg
      541 gtgcgccacg agagcatccg cgtacgctcg atctgttgtt cgagcatttg ctcgcgctcg
      601 tgggtggcct gcatcgcggc ggcgaggcgc gctcggaacg cggtgctcgg ctcagagctc
      661 ggacgcacgg cgcggcgcag actgattcga acggcgcgac cgaggcggac acgctcgacg
      721 cagcgcgaac actcttccaa gtgctgctct acctcgacca ccttctcggg caaaagctcg
      781 ccgtcgacga acggctcgag caacggaact acgctgcggc aggtcacgcg cgcgctcctt
      841 tctttcgacg gtaggcttcc atctcgacga ccggctcttc tttcttggga agctctccga
      901 cggtcgcctc cggcccaacc agcccgatcg cccgcgcgtg ctcgtagaga cgagacttca
      961 acatgcgtcg tccgcggtgc aggcgcgaca tcacggtgcc aatcgggcaa cccatgatgt
     1021 ccgaaatctc cttgtacgag aggtcctcga cgtcgctcag cacgaccgcg agacgaaact
     1081 cggtgggcag ctcgtcgaga gccttgccga tctcggcttc gaggatcggt cgcagcgcct
     1141 gcgactcagg atcgcgcaac gattccatcg tcgacgcgct cacccaacca tccgccagcg
     1201 gatcggcgtc gggcccgtcg agcacgctgc gttcgagccc gccgcgacgg taacggttga
     1261 tgaacgtgtt cgtgaggatc ttcagcagcc aggcgcgcag gttcgttccg gtctcgaact
     1321 gtccgcgcgc gcggatcgcc ttcaataccg cgtcttgtac gaggtcttcg gcttcgggcc
     1381 cgttcttcat caggcgcgag gccaccgaca agagcgacgg taggtgcagg agcacctcct
     1441 gctcgaactg ttggttactt agagcgcgtg aggccatggt ccgtggaata ccggtgcaac
     1501 cggcccccca cgctagctat tcccgggcgc ctcgggaggc ttttcgctag accggtcacc
     1561 gagccgacgc tcgatgctat ctagccgttc tgacagacgt ttcacctcgg tttcgaggtc
     1621 ccgaaacgct gcaaaattcg gcagcaccgc tcggatccgc tcgtcgatgg cggactgcca
     1681 ctgctccagg gtggcgcgaa ggcccttgtt ctccttgccc tgcgacatgt cctcttctcc
     1741 tggcactggc ggctccatgg gggcgcctgc tatttcgcct tcttcgcgag gaatcagttt
     1801 gccgatcggc ccttctcgaa gctgagtcaa aatgcgctcg ccgcggtggc gaatgagcgc
     1861 tttcagggtc gtcagcggga tggcgcgagg cttctcgcgc agctcttccg agatgatgag
     1921 ggcgagcgtt acgtccgttt tgtcctcttt cgtcgcgttg tcgatgatct ggacgtcctc
     1981 gccgtcccgg atcatctgcg cgatctgcaa gagggtgacg tagctgcttc ccttcgtgtc
     2041 gtagagcttg cggttggaat accgcttgat gatgcgccgc tgcgaaggcg gcgcctcact
     2101 ctcggagcct gcgtcgccat tcatggtcgg atcctgtagg ggtgcatgcg tgcgcgcggt
     2161 ggggttggta gccgcctcac gtcgacctga gatcgagtcg cccgctcaac ggccggcact
     2221 cttcagttca gcagccctta cgcagagcgt caaggggacc gccctgcgct tctatcccac
     2281 ctcatgtctc tgccgattct cgccgcaggt ttctgcacag gctcggcatt cggacgctgc
     2341 ggcaggggtc ggaccgcggg ctctcggcgc ccctcgaccg cgacgaacgc ctccgcccag
     2401 gcgccctcgg cgcgccgtac aagcatgcga cccacgtgga ctcttctctg aattccacct
     2461 atctcgtcgg cgctcccctc gctcggtcga ggagattcga gacgatggtc cttcgatgcg
     2521 tgccctaagt gttgcgtttc tcgcgttgtt cttcggtctc tctgcgctgc cgctcgcggc
     2581 tcgcggtctg aagatcgccg aagcaaaact cttcggggtg acgacgctca tcgagcctgt
     2641 cgacttgagt tggcccgcgt atcgcgacgg taagttccaa gagtcgttcg aggcgagctt
     2701 cgacgagagg ctcggctttc gcacgcacgc gatccgcgcg gacaacgagc tcaacctgcg
     2761 cgtctttcgt cagctcggct acgaccctca caccggccga gtgttgggca aagacgatta
     2821 catctacgat cgcgtttacg tagattcgtt caatcgtcgc gacgacgttc cgatcgaggc
     2881 catcgagccg cgcgtgaaga gcatgctcgc gcttcagcgg tacatggagt cgcgcggcaa
     2941 gaccctcttg gtgctgataa cgccgagcaa ggccgtgctc tacccagagt acctgccccg
     3001 acgcatgatc gaccgcgagc agcagcgcgg gccgtccaac tatcacaagc tgctcatcct
     3061 gctccagcgt tacggcgtgc actatttcga cgcgcagggc taccttgccg cgctgaaggg
     3121 caaggtgccg ttcaccctgt tcccgaactc cggcatccat tggggcgacc cggcggcgtg
     3181 tgaggtctcc gcgcgcgtgc tcgagcgcat cggcgagcaa ttgcagcgac gcgttccgcg
     3241 ccttcaatgc gagccgtatc gcccgtcagc cgatctgcaa cgggacgaca ccgacgtgct
     3301 cgacgtttgc aacttgtggt cacccgaacg cttggtgcgt cccggattct acgc
//
LOCUS       DASTKS010001232         5282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_643618, whole genome shotgun sequence.
ACCESSION   DASTKS010001232 DASTKS010000000
VERSION     DASTKS010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643618"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="VFQ35_26290"
     CDS             <1..1422
                     /locus_tag="VFQ35_26290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934250.1"
                     /translation="REVLGKSFLEAFPELQGTEMQAIVERVYRTGEPFVIRELSTPLR
                     NAEGVVETRHHTFNLAPIRNGEGAVTALMVVAVDMTEQVAARLSLERTDEERRRLLDA
                     ADAAARAKDEFLAMLGHELRNPLAPIMTALHLLKSKETPDIRREREIIERQATHLIHL
                     VDDLLDASRVAQGKIELKRSHVALSEVVAMAVEMAAPLFEKKNHRLLIDVPKAGLDLH
                     ADPMRLAQVVSNLLTNAARYTKAGGNIALTARREQDFALLKVEDNGIGIAPEHVAQLF
                     NMFFQAPQRRDRAEGGLGLGLALAKSLIELHGGNVSASSPGLGLGSVFEVRVPLAERP
                     PEPLLEPAPSPGVFRSGPSPSVLLVDDNRDLADMLAQFLEEAGCVVWTAHDGPSALAL
                     AKDVRPAVAVIDIGLPVMDGYELATSLREALSSAQPRLIAMTGYGEAEDRRRSESAGF
                     QCHLVKPVDVGLLLRALLPPATD"
     gene            complement(1525..3243)
                     /locus_tag="VFQ35_26295"
     CDS             complement(1525..3243)
                     /locus_tag="VFQ35_26295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9934251.1"
                     /translation="MRSLCMGLIEQDIILPFPEIPSGERETLHTVIGSLEQLFTPHEA
                     DFKKWDKQAEFPAEFIEELRQFGLFGLVIPEEHGGMGFGSAAYSRTLQEVATYDASVA
                     VTIGAHSSIGMRGLLLFGNEEQRQRFYPKLATGESIAAFCLTEPSSGSDAASIKTTAV
                     RDGDDWVLNGNKIWITNGGIADFFTVFAKTGKADDRAQLSAFIVTRDMPGVTTGPHED
                     KMGIRASSTTTVHFENVRVPAKNLLGEEGKGFKVAMMILNSGRTGLGGGSVGGMKRLI
                     RMAAQQAKGRVQFGKPISEFGLIKQKVGQMVVDCYATEAAVSMVAGLIDQGYEEYAVE
                     AAISKVFASEALNRAADEALQIAAGNGYMTEYPYERVVRDSRINRIFEGTNEILRLFI
                     ALTAMNDVGQNLRELAKTLEGVFADPIKGFGVLSEYALRRVSLATGYTREGTVFTRLH
                     PALKAQQQVFEESTRDLGSAVDRILRKHGRNIIDKQFATRRLGDMMIDLFVSACVLSR
                     VDAAVRANGPEKSPRELDIAHTFLAQARKRIRQNVADIDSNEDEQIKALADHAFEAEK
                     FPWDTL"
     gene            3600..4697
                     /locus_tag="VFQ35_26300"
     CDS             3600..4697
                     /locus_tag="VFQ35_26300"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9934252.1"
                     /translation="MTASLGSWQDRAVQPILSPPPTKGHLSVARLARFWYVAFPSTTL
                     RRRPMARTLLGTPLVFFRDNDGRARALLDRCPHRNVPLSIGRVAGNGRLECAYHGWQF
                     DGDGQCRSVPGLIEEGAPRERRCPRAHVREVDGLVWVYGALDQEPEGEPFANTPLGDG
                     YTRVIREVEADATLHATLENALDVPHTAFLHRGLFRGGERHEVRVRVRRSGTRVEAEY
                     MGEPRPSGVVGRLLSPGGGVVEHWDRFFLPSIAQVEYRLGDDAHFRVTAFGVPVTDFR
                     TRLIAVVDFRTPLPGRLLKPLLEPVALRIFGQDKKILQAQTATIERFGGEQYQSTEVD
                     FLGPHIWRLLKQAERGEPGENVVESEVRFFA"
BASE COUNT          867 a         1676 c         1726 g         1013 t
ORIGIN      
        1 cgcgaggtgc tcggaaaatc attcctcgaa gcgtttccgg agctacaggg cacggagatg
       61 caggccatcg tcgagcgcgt gtatcgaacc ggagagccat tcgtgattcg agagctctcg
      121 actccgcttc gcaacgccga gggagtcgtc gaaactcgcc atcacacctt caacttggca
      181 ccgatccgga acggcgaggg tgcggtgacc gcgctcatgg tcgtggccgt cgacatgacc
      241 gaacaggtag cggcgcgact gagcctcgaa cgaacggacg aagaacggcg acgcctgctc
      301 gacgccgccg acgctgccgc gcgagcgaag gacgaatttc tcgcgatgct cggacacgag
      361 ctcagaaatc cgctggcgcc gatcatgacg gcgctacacc tgctgaaatc caaagagacc
      421 cccgacattc gacgcgagcg agagatcatc gaacgtcaag cgacgcactt gatccacttg
      481 gtcgacgatc tgctcgacgc gtcgcgcgtc gcgcaaggca aaatcgagct caagcgtagc
      541 cacgtggcgc tcagcgaggt cgtcgcgatg gccgtagaga tggcggctcc cctcttcgag
      601 aagaaaaacc accggctgct catcgacgtg ccgaaagccg gtctcgacct gcatgcggac
      661 ccgatgcgct tggcgcaagt cgtctcgaac ctgttgacca acgccgcacg ctacacaaaa
      721 gcgggcggga acatcgcgct gaccgctcgg cgcgagcaag acttcgcgct cctcaaggtc
      781 gaagacaatg gtatcggtat cgctccggag catgtcgcgc agctcttcaa catgttcttt
      841 caggcgccgc agcgccgcga tcgtgccgaa ggcggcctcg gtctcggctt ggctctagcc
      901 aagagcttga tcgaattgca tggtggcaac gtgagcgcgt cgagcccagg cctcgggctc
      961 ggaagcgtct tcgaggtgcg ggtcccgctc gcggagcgcc ctccagaacc cctcctcgag
     1021 ccggcgccga gtccgggcgt gttccgttcc gggccatcgc ccagcgtttt gctggtcgac
     1081 gacaatcgcg atcttgccga catgctggcc cagttcctcg aggaggctgg ctgcgtcgtg
     1141 tggaccgcgc acgacggtcc ctccgctctc gccctcgcca aagacgtgag gcctgccgtc
     1201 gccgtgatcg acattggctt gccagtgatg gacggctacg agcttgccac gtcgttgcgc
     1261 gaggcgctca gttcggctca gccgcggctc atcgcaatga ccggatacgg cgaggccgag
     1321 gatcggcggc gcagtgagag cgccggcttc cagtgtcacc tcgtgaagcc ggtggacgtg
     1381 gggctcttgc tgcgcgcact gctgccgccc gcaaccgact gacctcgctt ggcttaccgc
     1441 gccggcgtcc tcgtgcatgc ggctcgcgcc gtgaggatgg ccttcaatcc gcgcgacccg
     1501 aacgctagcg acgaaggtgc ctcgctacag cgtatcccaa gggaattttt cggcctcgaa
     1561 cgcgtgatcc gcgagcgcct tgatctgctc gtcttcgttc gagtcgatgt cggccacgtt
     1621 ctggcggatg cgctttcgcg cttgagcgag gaaagtgtgc gcgatatcga gctcgcgcgg
     1681 gctcttctcc ggcccgttcg cgcgcacggc cgcgtccact cgcgagagca cgcacgcgct
     1741 cacgaagagg tcgatcatca tgtctccgag ccgcctcgtc gcgaactgtt tgtcgatgat
     1801 gttgcggcca tgcttgcgca aaatgcggtc gacggcggac cccagatcgc gcgtcgactc
     1861 ttcgaagacc tgctgctgtg ccttcagggc tgggtgcaag cgcgtgaaca ccgtaccctc
     1921 gcgcgtgtag ccagtcgcca gcgagacgcg ccgcaaggcg tactcgctga gcacgccgaa
     1981 gcccttgatg ggatccgcga aaactccctc gagcgtcttt gcgagctcgc gcaggttctg
     2041 tccgacgtcg ttcatggccg tgagagcgat gaacaggcgc aggatttcgt tggtgccctc
     2101 gaagatgcgg ttgatgcggc tgtcgcgcac cacgcgctcg tacgggtact cggtcatgta
     2161 gccgttgccg gccgcgatct gcagcgcctc gtccgctgcg cggttcagcg cctcgctcgc
     2221 gaacactttg ctgatggcgg cctcgaccgc gtactcctcg tagccttggt cgatgagccc
     2281 tgcgaccatc gaaacagctg cttcggtggc gtaacagtcg accaccattt ggccgacctt
     2341 ctgcttgatt agcccgaact cggagatcgg ctttccgaac tggaccctgc ctttggcctg
     2401 ctgcgcggcc attctgatga ggcgcttcat tccgccgacc gagccacctc cgaggcccgt
     2461 gcgcccggag ttcaagatca tcatcgcgac cttgaatcct ttgccttctt cgcccagcaa
     2521 attcttggcg ggcactcgca cgttttcgaa atggacggtg gtggtggagc tcgctcggat
     2581 ccccatcttg tcttcgtgcg gcccggtggt aacgccaggc atgtcgcgcg tgacgatgaa
     2641 ggcgctgagc tgcgcgcggt cgtccgcttt accggtcttg gcgaagacgg tgaagaagtc
     2701 tgcgattccg ccgttggtga tccaaatctt gttgccgttc agcacccagt cgtcgccatc
     2761 acgcaccgcc gtcgttttga tcgacgccgc gtccgagccg ctgctcggtt cggtgagaca
     2821 gaaggccgcg atgctttctc ccgtcgcaag cttcgggtaa aagcgctgtc gttgctcttc
     2881 gttgccgaac aagagcaagc cgcgcatgcc gatcgagctg tgcgcgccaa tcgtgaccgc
     2941 gaccgaagcg tcgtaggtgg ccacctcttg tagagtgcgc gaataggcgg cgctcccgaa
     3001 gcccatgccg ccgtgctctt ccgggatcac gagcccgaag agcccgaatt gccgcagctc
     3061 ttcgatgaac tcggctggaa attcggcctg cttgtcccac ttcttgaagt cggcttcgtg
     3121 gggggtgaag agctgctcga gcgagccaat gacggtatgc agcgtttcgc gctcgccgct
     3181 cgggatctcg gggaagggca gaatgatgtc ttgctcgatg agccccatgc aaagcgaccg
     3241 catgaagctg gctgtattct tgtcgaccac cctggcctcc ttcggcgaac ggcggagttc
     3301 gcagctttcg tcactatggg gggccctcgc tttgcatgca agtcgtgtgc gacgaaagca
     3361 acgcccgtcc caagcgtgcg cggatgtagc agcggcgact gctgctctgc cggcaccgca
     3421 tcgagcgcgg tgcgcaggcc ggctctctga agctgcgacg ccgacagcac cgaacggccc
     3481 ccgggctgcg tccgtccccg cgtgttcctc cgcattcgcc cgcagccacg cccgctgcgt
     3541 ccgtccccgc gtgttcctcc gcattcgccg cagccacgcc cgcgcggaag gatggtcgga
     3601 tgacggcgtc tctgggatcg tggcaagatc gcgcggtgca gccgattttg agccctccgc
     3661 ccacgaaagg gcatctctcg gtcgcgcgcc tggcgcgctt ttggtacgtg gcgtttccct
     3721 ccaccacgct tcgccgtcgc ccaatggcgc gcacgttgct cgggacgccg ctcgtatttt
     3781 tccgcgacaa cgacggacgt gcacgagcgc tcttggaccg ctgcccgcac cgcaacgtgc
     3841 cgctctcgat tgggcgcgtt gcgggaaacg ggcggctcga gtgcgcttat cacggttggc
     3901 aattcgacgg cgacggccaa tgtcgcagcg tcccggggct catcgaggaa ggcgcgccgc
     3961 gtgagcgacg ctgtccgcga gctcacgttc gcgaggtcga cggtttggtt tgggtgtacg
     4021 gggccctcga ccaggagccg gaaggtgagc cgttcgcgaa tacgccgttg ggtgacggct
     4081 acacgcgcgt gatccgagag gtcgaggccg acgcgaccct tcacgcgaca ctcgaaaatg
     4141 cgctcgacgt gcctcatacg gcgtttttgc atcgcgggct atttcgcggc ggcgaacgtc
     4201 acgaggtgcg agtgcgtgtt cgtcgcagcg gaactcgcgt cgaagcagag tacatgggag
     4261 aaccgcggcc gagcggtgtc gttggtcgct tgctctctcc cggcggagga gtcgtcgaac
     4321 attgggaccg cttctttttg ccctccatcg cccaagtcga gtaccgactc ggcgatgacg
     4381 cacattttcg cgtcaccgcc ttcggcgtgc cggtcaccga cttccgaacg cgactcatcg
     4441 ccgtagtcga ctttagaacg ccccttccag gtcgcctgct gaagccgctg ctagagccgg
     4501 tggctctgcg catcttcgga caagacaaga aaatcttgca ggcgcagacc gccacgatcg
     4561 agcggttcgg cggcgagcag tatcaatcga ccgaagtcga cttcctcggg ccgcacattt
     4621 ggcgcctgct caagcaagcc gagcgcggcg aaccggggga gaacgtcgtc gaaagcgaag
     4681 tgcggttctt tgcttaggcc gaactcggtt ggcccttggc ggcgtacctg ctcgcgacgt
     4741 gtgacgaatc ctgagacttc cgtaagctca gtagccctcg tccatcgtct cgcctcggac
     4801 tactcacgtc tcttcgcgag gctcggtcgg gttttgggac tggctcgcaa acggaggacc
     4861 agagaaagtg atggaacagg gtctagccct ccctagcggt tctggacgtt ttgacaaaaa
     4921 gtgtcaacac gtcaaaacgc cttcggcccc tcaacagcgc ttgcttttgg gggtggccgg
     4981 cagctgaacg gcgattcgcg attcgacccc gcccgggcgg cgcggttcgg gctggcggcg
     5041 cgttcgggga acggcgcgtt cggggcgcgg tgcggtttgg gaacggcgcg gtttgggaac
     5101 ggcgcgttcg gggcgcggtt tgggaacggc gcggtttggg aacggcgcgg tttgggaacg
     5161 gcgcgttcgg ggcgtggttt gggaacggcg cgttcggggc gcggtgcggt ttgggactag
     5221 ggtggggggg ggcgtgcgaa gggtcgtggt tagcgggtca ggggtcgtcc actgggggct
     5281 gg
//
LOCUS       DASTKS010001233         8975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_198603, whole genome shotgun sequence.
ACCESSION   DASTKS010001233 DASTKS010000000
VERSION     DASTKS010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198603"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFQ35_26305"
     CDS             <1..906
                     /locus_tag="VFQ35_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934253.1"
                     /translation="AADASAPPKLVERVERTRLFYDADGQLIRTEVATNDHVQSRVLA
                     ERDRPAVPIVVALSAGGSYQSDTELYDASAGLGIHRRPKVQRYGADPLEVGLDAAFKF
                     HRASGITSTDQTTLRFGVDYHDILPRITLFSFATTDRNLPANLRLNLEVAVLGVKFEF
                     VPRGQYQLDLSFAPVWNFRSIIAPDMMGATTDENTSKLRGSFRARAGLYRATWSLLDT
                     FEFLPTLFGDDVAQENKFWNRTVIRNTLAFDVTLTRHLTLREELKYTRDPAMRAQASC
                     PDSSNPLCRGYAVTNTTSLVLNVEL"
     gene            1082..1606
                     /locus_tag="VFQ35_26310"
     CDS             1082..1606
                     /locus_tag="VFQ35_26310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934254.1"
                     /translation="MNSRAVWVTLFLAGALLAGCTSTGTATGQLVSPSGKREAVKFNW
                     RSDAGDWHHGKISVALPNGEHYSGTYRQVSQVVPVDAYASMWVGWRPFWPDWPTPWYR
                     GTVAPDWRGWTRVYEGRVVARLESGNPDRAMRCRFELDDPTNGLSGGGHGECRLSDGS
                     SIEGAEIFPKEDSD"
     gene            1748..2713
                     /locus_tag="VFQ35_26315"
     CDS             1748..2713
                     /locus_tag="VFQ35_26315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934255.1"
                     /translation="MTSRWHNLSLFLGLTVICVSVSCGGRSDRDGSDGGSAGETPSSG
                     GRASVLGGAPSFGGEPAHSGNTGHGGAPASGGAPAYGGAPPAKGGNPGNGGAPASGGS
                     SPGGSPGVGGALSGGATGVGGAMNQCPTFAMQMSQLVEEAKKCNPVLSSEQCTQLIEP
                     GLNCACPTFANPQQKDAIQKLKSVYESYIELGCGMTVVCGPCLEPNRGYCSPQGRCED
                     VAQAQPLSCKVGGVIYPSGTTQIKDPGGPCNTCSCNDGVLTCTEIGCPSGCPSGTAFG
                     QGCAQCGPADGCLLPEYECYPVCTTTCPDANQFCLNGLCRSGFCG"
     gene            2716..3009
                     /locus_tag="VFQ35_26320"
     CDS             2716..3009
                     /locus_tag="VFQ35_26320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934256.1"
                     /translation="MAHESKGFELLEQEILQAKAEALGRAGEKLRRAVGAYREAQATL
                     DETPSARSELERRWQEVMRAREVLIVLREGIGLRQHDSVDAEYAIPLASRGSI"
     gene            complement(3041..4105)
                     /locus_tag="VFQ35_26325"
     CDS             complement(3041..4105)
                     /locus_tag="VFQ35_26325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9934257.1"
                     /translation="MAPPSSDRRFTTPAREEHSESANARIVLRSQQVVPRLTTSALAA
                     FPSSRPARARDKMVLFVIQGHQQGEVMTVEGESVLVGRDNGAQLRLDDDTVSREHARI
                     RVEEGQAYVEDLRSLNGTFVNERRIDAVTVVRDGDQLRFGDNTIVKFWTLDEIEHAAL
                     TTLYEHTSRDALTRLYNRRYVDERLQSEFSFARRHRVWLSILLIDIDYFKRVNDNFGH
                     ATGDAVLKSVAANLLAVTRPEDVVARYGGEEFLLIARNTSRVSATALAERVRRAVQAA
                     RIPGQTFEVTASVGVVSAGPGNFPDSPEKLLGAADDALYSAKDQGRNRVAVARPLTES
                     DAPVSSPDTIPPGRFDPVRR"
     gene            4413..4919
                     /locus_tag="VFQ35_26330"
     CDS             4413..4919
                     /locus_tag="VFQ35_26330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934258.1"
                     /translation="MGYFNDNPRIPIAALSIEALRAQVGRVEAVAGTREDNALIVDPG
                     DHRRQVWVKPAYSGYRSAWRHVHIHIPAYMDVDHIHNRARALAQGYAYVRLFLCNSDA
                     NQRHGRFLERKLTHSARARARTAAYCDILFASNWTIAKMAGIAIDRDNPTYDQRAALD
                     WLNANNFI"
     gene            complement(4958..8944)
                     /locus_tag="VFQ35_26335"
     CDS             complement(4958..8944)
                     /locus_tag="VFQ35_26335"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9934259.1"
                     /translation="MSSSPYIVGIGASAGGLEALERFFDSVPNASGMAYVVVQHLSPD
                     FKSLMDEILARRTKIPVQLVEDGIVVEPDHIYLMPPKKEMIISGGRLLLSERDRDQDL
                     SLPIDVFFRSLAQDCGSRAIAVVLSGGGSDGSRGIRAVHKAGGLVVIQDVDSAQFDGM
                     PKTARDAGVADYVLPPQEMASVLLEHVNRAGQPSERAVARVQGMSAVYRMLEEEFGID
                     FTHYKPSTVTRRIERRLQLAQVEDIEVYVKKLRGERKELDALYRDLLIGVTRFFRNEE
                     AFDVLEKEVLPELLRNGPLDAPFRVWVAGCATGEEVYSLAILLHELTQESGRPFKIFA
                     TDVHRGSLEHATRAFYDEEAVVNVSPERLKRYFLPRGRGYQVVPDLRQTVVFAHHNVI
                     KDAPFTRVDLISCRNMLIYLQPPAQEKVLSLFHFALNRGGMMFLGPSESLGHLDHDFE
                     TIDKTWRIYRKFSDSRTSVERRVPPPRKGSEIRASTSSGLLGTNHARHSVSQLLGTYD
                     AMLDELLPASILIDDHGELLHTFAGATRFLRLREGRQALDVYEMVDPELKMLLLTGVQ
                     RAMQEDAAIVFNGVRCEEGVCKLTIRRIRNKKTPAAHILVSFEVTDGAGEPPPNQVEI
                     DIRQISREQLGAIEAELTRTKENLQAATEELETSNEELQAANEELLASNEELQSTNEE
                     LQSVNEELYSVNAEYQRKIADLTELANDMDNLLSSTDVGTIFLDRELKIRKFTPQISE
                     AFNLLPQDVGRPIETFTYAVEHPELNEDLRRVLHTGERVERELRDRHGRAFFLRILPY
                     RAKGSVDGVVLTLIDVSGLKAAEDALFHERYLLNSLLQSIPDAIYFKDARGRFIRTNP
                     AMAERLGLSDPRQAAGKTGFELSSHEAALAVHEQDDAVLRSGTAQHYKLEKRELPSGE
                     VGWDLVTRLPLTDSASRIVGIIGIFRSVTEQKRAEEKINDAVRRRDEFLAMLSHELRN
                     PLGAIVSATGLLKADAEPPNARGKLLNIVERQAQQMARLLDDLLEASRVTQDKIELRK
                     RVLDVRGVARDALDAIRNVLESRGLELSAEITPEPLCVEGDAARLQQICVNLLSNAAK
                     YTPRGGKVLFQVAREGDAALVRVRDNGMGIPKEMLDSVFELFVQSSRTLDRAEGGLGV
                     GLTLVRGLVAKHGGTVVARSDGPGKGSEFLVRLPLTSAAGVAEPAPSSGGPKLARGSR
                     VVVIEDNPDSREMLCSLLTHAGFDCKSSDNGATGVALIDEFKPVVAIIDIGLPGLDGL
                     EVARRVRQNSAHEGIYLVALTGYGQRADREMALKAGFDEHLVKPVDFATIERVLGTSK
                     EASSKLVN"
BASE COUNT         1567 a         2966 c         2860 g         1582 t
ORIGIN      
        1 gccgccgacg cgagcgcgcc gcccaagctg gtcgagcgag tcgagcgtac gcggctcttc
       61 tacgacgccg acgggcaact catccgcacc gaagtggcga ccaacgacca cgtgcagagt
      121 cgcgtgctcg ccgagcggga ccgtcccgca gtgccaatcg tggtcgcgct cagcgcgggc
      181 ggctcttatc aaagcgacac cgagctctac gacgcgagcg ccgggctcgg catccaccgg
      241 cgccccaagg tgcagcgata cggcgccgat ccgctggaag tcgggctgga cgccgcgttc
      301 aagtttcatc gcgcgtcggg cataacgagc accgaccaaa ccacgctgcg cttcggcgtc
      361 gactaccacg acatcttgcc gcgcatcact ctcttcagct tcgcgaccac ggatcgcaac
      421 ctgcccgcca acctgcgcct caacctcgaa gtcgccgtgc tcggcgtaaa gttcgagttc
      481 gtgcctcggg ggcaatacca actcgacctg agctttgctc ccgtctggaa ctttcgctcc
      541 atcatcgccc ccgacatgat gggcgccacc accgacgaaa acacttccaa gctgcgcggc
      601 tcgttccgcg cgcgcgcagg gctctaccgc gcgacctggt cgctgctcga caccttcgag
      661 tttttgccga ctctgttcgg cgacgacgta gcccaagaaa acaagttttg gaatcgcacg
      721 gtcatccgca acacccttgc gttcgacgtg accctcaccc gacacctcac cttgcgcgaa
      781 gagctgaagt acacgcgcga tccggccatg cgcgctcagg ctagctgccc cgactcctcc
      841 aatccgttgt gcaggggtta cgccgtcacc aatacgactt ctctcgtttt gaacgtggag
      901 ctctgagcac cgcgccgcgc gctcgctgcg cgctgtgccg gcgacggcgg aggcgctcgc
      961 tcgccgcgct cgaggcgttc aggcaaaggg gccgatcgcg ccaccgctac gagatgatcg
     1021 cgtcactttt acgagtcgcg gaaggaactc gccggtacgt gcgttgcact cgtacgtccc
     1081 catgaactct cgagctgtgt gggttaccct ctttctcgct ggtgcgctgc tcgctggctg
     1141 caccagcact ggtacggcga cggggcagct ggtgtcgccg tcaggaaagc gtgaagccgt
     1201 gaagttcaat tggcgctcgg acgcgggtga ttggcaccac ggcaagatct cggtcgcgct
     1261 cccgaacggc gagcactaca gcgggacgta tcggcaagtc tcgcaagtcg tccccgtcga
     1321 cgcctacgcg agcatgtggg tcgggtggag gcccttctgg ccagactggc cgaccccttg
     1381 gtatcgcgga acggtcgcgc cggactggcg gggctggacg cgcgtttacg agggacgcgt
     1441 cgttgctcgc ctcgaatctg ggaatccgga tcgcgcgatg cgctgtcgct tcgagctcga
     1501 cgaccccacc aacggtctct ctggcggtgg gcacggcgag tgtcggctca gcgacggttc
     1561 gagcatcgag ggcgcggaga ttttcccgaa ggaagactcc gactgacctg gcgacgggtg
     1621 tgcgcaaagg agcgcaactt cagcgggcct cactggttca ctcggcgcag gcccggcaat
     1681 cgcggttgat cgcgggcatc ggcagagttg ttgctacgat gtgtcatcag cgaaggaacc
     1741 gcctgaaatg actagtcgct ggcacaactt gagcctcttt ttgggtctga ccgtgatctg
     1801 cgtgtctgtg tcgtgcgggg gtcgctcgga ccgcgacggc agcgacggag gcagcgcagg
     1861 cgagactcca agcagcggtg ggcgcgcttc tgtcctgggc ggagctccca gttttggcgg
     1921 cgagccagcc cacagcggca acaccggcca tggcggcgct cccgcaagcg gcggcgctcc
     1981 agcctacggc ggcgctccac cagcaaaggg cggaaacccc ggcaacggcg gagctcccgc
     2041 gagcggcggg tccagtcctg gtggctcgcc gggcgttggc ggcgcgctga gcggcggagc
     2101 gacgggcgtg ggcggcgcca tgaaccaatg ccccacgttc gcgatgcaaa tgtcgcagct
     2161 cgtcgaagag gcgaaaaagt gcaaccccgt gctcagctcg gagcagtgca cccagctcat
     2221 cgagcccggg ctgaactgcg cctgcccgac tttcgccaat cctcagcaaa aggacgcgat
     2281 acaaaagctc aaaagcgttt acgaaagcta tatcgagctc ggttgtggga tgaccgtcgt
     2341 gtgcggcccc tgcctcgaac cgaaccgcgg ctactgctcg ccccaaggcc gttgcgaaga
     2401 cgtagcccaa gcccagccgc tcagctgcaa agtgggcggg gtcatctatc cctcggggac
     2461 gacgcaaatc aaagaccccg gaggcccctg caacacgtgt tcgtgtaacg acggcgtact
     2521 gacgtgtacc gaaatcggtt gtccctcggg ctgcccgtcc ggcaccgcgt ttgggcaagg
     2581 ctgcgcccaa tgtggccccg ccgacggctg cttgcttccc gaatacgagt gctatccggt
     2641 gtgcacgacg acctgcccgg acgccaacca attttgcctg aacgggctct gccgctcggg
     2701 cttttgtggc tgaacgtggc tcacgagtcg aagggcttcg aactcctcga gcaggagatc
     2761 ttgcaggcca aggccgaagc cctcgggcgc gcgggcgaaa agctcaggcg cgccgtaggg
     2821 gcataccgag aggcgcaagc cacgctcgac gagaccccga gcgcgcgcag cgagctcgaa
     2881 cggcgctggc aagaggtgat gcgagcccga gaggtgctga tcgtcctgcg tgaagggatc
     2941 ggtctcaggc agcacgactc cgtcgacgcc gagtacgcca ttccgctcgc gagccgcggt
     3001 agcatctgag caagccgcgg agcggcactc ggagccgcgt tcagcgacga acggggtcga
     3061 atcgcccggg cggaatcgta tcggggctcg agacgggagc gtcggattcg gtgagaggcc
     3121 gcgctacggc aacgcgattt cggccttggt ccttcgcgct gtagagcgca tcgtcggcgg
     3181 cgccgagcag cttttcgggc gaatcaggga agttgccggg gcccgccgag acaacgccaa
     3241 cgctggccgt gacttcgaag gtttgccccg ggatgcgagc cgcctggacc gcgcgacgca
     3301 cgcgttcggc gagggcggtg gcgctcacgc gggacgtatt gcgggcgatg agcaagaact
     3361 cctcaccgcc gtagcgagcc acgacgtctt cgggtcgcgt caccgcgagc aggttcgcag
     3421 cgaccgattt gagcacggca tcccccgtag cgtgaccgaa gttgtcgttg acgcgcttga
     3481 agtaatcgat gtcgatgaga aggatcgaaa gccagacgcg atgacggcgc gcaaacgaga
     3541 actcgctttg caaccgctcg tcgacgtagc gacgattgta cagtcgcgtc aacgcgtcac
     3601 gggacgtgtg ctcgtacagc gtcgtgagcg cggcatgctc gatctcgtcc agcgtccaaa
     3661 acttcacgat cgtgttatcg ccgaaccgga gctgatcgcc atcccgcacg accgtaaccg
     3721 catcgatgcg gcgctcgttc acgaacgtcc cgttgaggct cctcaaatct tcgacgtacg
     3781 cttgcccctc ctcgacgcgg attcgagcgt gctcgcgcga aacggtgtcg tcgtcgagtc
     3841 gtagctgagc cccgttgtcg cgacccacca gcaccgattc gccctccacc gtcatgacct
     3901 cgccctgctg atggccctga atgacgaaca agaccatctt gtcgcgcgcg cgcgcagggc
     3961 gcgaagaagg gaacgccgca agggcgctcg tcgtcaggcg cgggacgacc tgctgcgagc
     4021 ggaggacaat gcgagcgttt gccgactcgc tgtgttcctc ccgcgcgggc gtcgtaaagc
     4081 gccggtcgga ggagggcgga gccatttatc gacgattcgt aagcaacctg cgggccaacc
     4141 actaagcgcc gagtttcgtc agcgcgctgg gcccgaaagg cgcaagcgct gcgcccaagc
     4201 cgagccattt tgcggcattt gcgctcgaac gcagcgcttt cgggtctcga aacgagccaa
     4261 ttgcgaaggc tgactgactc ggcgaggcca aaaggagaat ccagcgagcg ccgtcgattt
     4321 cacaaccgcg acggaaacgc cgccccgtcc cgccgtcgcg gtcagcgact ccaagattga
     4381 ctcacccgag aggcccgagg tctaacggag cggtggggta cttcaacgac aatccaagga
     4441 tcccgattgc agccttgagc atcgaggcgc ttcgcgccca ggtggggcgc gtcgaggccg
     4501 tggctggcac gcgcgaagac aacgcgctga tcgtcgaccc aggtgatcat cgacgccaag
     4561 tgtgggtcaa gccggcctac agcggctacc ggagcgcgtg gcgccacgtg cacattcaca
     4621 tccccgcgta catggacgtg gaccacatcc acaaccgagc gcgggcgctc gcgcaaggat
     4681 acgcctacgt ccggctgttc ttgtgcaaca gcgacgcgaa ccagcgccac ggccgctttc
     4741 tcgagcgcaa gctcacgcac agcgcgcggg cgcgcgctcg cacggcggcg tactgcgaca
     4801 tcctcttcgc ttcgaactgg accatcgcca agatggcagg catcgcgatc gaccgggaca
     4861 acccgaccta cgatcaacgc gccgcgctcg actggctcaa cgccaacaac ttcatctgag
     4921 ctccgacgca gttggggcgg cgctggctcg gagagcccta gttcactagc ttcgagctcg
     4981 cttctttgct cgtgccgagc acgcgctcga tggtcgcgaa atcgaccggc ttcacgaggt
     5041 gctcgtcgaa gccggccttg agggccatct cgcgatccgc gcgttggccg tagccagtta
     5101 gagcgacgag gtaaatacct tcgtgggcgg agttttggcg gactcgccgc gcgacctcga
     5161 gcccgtcgag ccccggcaaa ccgatgtcga tgatcgcgac gaccggtttg aattcgtcga
     5221 tcaacgcgac gccggtcgcg ccgttgtccg acgacttgca gtcgaaccct gcgtgggtga
     5281 gcagcgagca caacatttcg cggctgtccg gattgtcctc gatgacgacc acgcgcgagc
     5341 cgcgcgcgag cttcggcccc cccgaagaag gagccggctc ggctaccccc gcggcgctcg
     5401 tgagcggcaa ccgcacgagg aactcgctgc ctttgccggg gccgtcgctg cgcgcgacca
     5461 cggtgccccc gtgcttcgct accagcccgc gcacgagcgt caggccaacg ccgagtccgc
     5521 cttcggcgcg atcgagggtc ctgctcgact gcacgaagag ctcgaagacg ctatcgagca
     5581 tctccttcgg gatccccatg ccgttgtcgc ggacgcgaac cagcgccgcg tcgccttcgc
     5641 gggcgacctg gaatagaacc ttgcccccgc gcggcgtgta cttcgccgcg ttgctcagca
     5701 aattcacgca tatttgctgg aggcgcgccg cgtcgccttc gacgcagagc ggctccgggg
     5761 tgatctctgc ggagagctcg aggccccgtg actccagcac gttgcgaatc gcgtcgagcg
     5821 cgtcgcgagc gactccgcgc acgtcgagca ccctcttgcg cagctcgatc ttgtcctgag
     5881 tcacgcggct cgcctcgagc aagtcgtcga gcaggcgcgc catctgctgg gcttggcgct
     5941 cgacgatgtt cagcagtttg ccgcgcgcat tgggcggctc cgcgtcggcc ttgagtaagc
     6001 cggtcgcgga cacgatcgcg ccgagcggat ttctgagttc gtgcgacagc atcgccaaaa
     6061 actcgtcccg ccgtcggacc gcgtcgttga tcttctcttc ggcgcgcttc tgctccgtca
     6121 cgctgcgaaa gattccgatg atgccgacga tgcgtgaagc gctgtcggtg agcggcagtc
     6181 gtgtgacgag atcccacccg acctctccgc tcgggagctc tcgcttttcg agcttgtagt
     6241 gctgggccgt gccgctgcgc agcaccgcgt cgtcctgctc gtgaacggcg agcgccgctt
     6301 cgtggctcga caactcgaag cccgtcttgc cggcggcttg ccggggatcg ctcagcccta
     6361 accgctcggc catggctggg ttcgtacgga tgaagcggcc gcgcgcgtcc ttgaagtaaa
     6421 tcgcgtccgg aatgctctgg agcaagctat tcaacaggta ccgctcgtgg aagagcgcgt
     6481 cctccgctgc cttcaggccg ctcacgtcga tgagcgtgag cacgacgccg tccaccgacc
     6541 ccttggcgcg atagggcagg attcgaagga agaaggcgcg gccgtgccga tcgcgcagct
     6601 ctcgctcgac gcgctccccg gtgtgaagaa cacgccgcaa gtcctcgttc agttcggggt
     6661 gctcgacggc gtacgtgaac gtctcgatgg gtcgcccgac gtcttggggc agcagattga
     6721 acgcctccga aatctgcgga gtgaactttc ggatcttgag ctctcgatcg agaaatatcg
     6781 taccgacgtc cgtgctcgag agcaggttgt ccatgtcgtt cgcgagctcg gtcaaatccg
     6841 cgatcttgcg ctggtactcc gcgttcacgg agtagagctc ttcgttcacg ctctggagtt
     6901 cttcgttggt gctctggagc tcctcattgg aagccaagag ctcttcgttc gcggcctgca
     6961 gctcctcgtt gctggtctcg agctcctccg tcgccgcctg caggttctcc ttggtgcgcg
     7021 tcagctcggc ctcgatcgcg ccgagttgct cccgtgaaat ctgccggatg tctatttcga
     7081 cttggttggg gggtggctcc cctgcgccgt ccgtgacttc gaacgagacc aagatgtgag
     7141 cagcgggcgt ctttttgttg cgaatgcgcc gaatcgtcag cttgcagacg ccctcttcac
     7201 aacgcacacc gttgaagacg atcgcggcgt cctcctgcat tgcgcgctga acgccggtca
     7261 agagcagcat cttgagctcg gggtccacca tctcgtagac gtcgagcgct tggcgcccct
     7321 cgcgcagcct gaggaaccgc gtcgcgcccg cgaaggtgtg aaggagttcg ccgtgatcgt
     7381 cgatcaggat gctcgccggc aacagctcgt cgagcatggc gtcgtacgtg ccgagaagct
     7441 gagacaccga gtggcgcgcg tggttcgtgc cgaggagccc gctcgatgtg ctggcgcgga
     7501 tctcggagcc tttacgcggc ggcggcacgc gccgttcgac cgaagtgcgc gagtcactga
     7561 actttcggta gattcgccag gtcttgtcga tggtttcgaa gtcgtgatcg aggtgaccga
     7621 ggctctcgct cggcccgagg aacatcattc cgccgcgatt caacgcgaaa tggaacaggc
     7681 tcagcacctt ctcctgcgcg ggcggctgca ggtagatgag catgttgcga cagctgatga
     7741 gatcgacgcg cgtgaacggc gcgtccttga tgacgttgtg gtgagcgaac accaccgttt
     7801 gccgtaagtc cggaacgacc tggtaccccc ggccccgcgg caagaagtag cgtttgaggc
     7861 gctccggcga cacgttgacg accgcctcct cgtcgtagaa cgcgcgcgtc gcgtgctcga
     7921 gcgagcctct gtggacgtcg gttgcgaaga tcttgaaggg gcggccgctc tcctgcgtca
     7981 attcgtgaag caagatggcg agcgagtaca cctcttcgcc cgtcgcgcaa ccagcgaccc
     8041 atacccggaa cggcgcgtcg agtgggccgt tccggagtag ctcgggcagc acctcctttt
     8101 cgagcacgtc gaacgcctcc tcgttgcgga agaagcgagt gaccccgatg agcaagtctc
     8161 gatagagcgc gtcgagctcc ttccgctcgc cgcgcaactt ctttacgtaa acctcgatgt
     8221 cctcgacctg cgcgagctgg agtcggcgct cgatacgccg cgtcaccgtg ctcggcttgt
     8281 agtgcgtgaa atcgatgccg aactcttcct ccagcatgcg gtaaacggcg ctcattccct
     8341 gcacgcgcgc caccgcccgc tcactcggct gccccgcccg gttcacgtgc tcgagcagca
     8401 cactcgccat ctcctgcggc ggcaagacgt agtccgcgac acccgcgtcg cgcgcagtct
     8461 tgggcatgcc gtcgaactgc gcactgtcca cgtcctgaat caccacgagc ccgccggcct
     8521 tgtggaccgc cctgattccg cgtgaaccgt cgctgcctcc gcccgagagc acgaccgcga
     8581 tcgcgcgcga cccgcaatct tgcgcgagtg accggaagaa cacgtcgatg ggcaagctga
     8641 gatcttggtc gcgatcgcgc tcgctcagga gcaagcgccc gcccgagatg atcatctctt
     8701 ttttcggcgg catcaggtag atgtggtccg gctcgacgac gatgccgtcc tccacgagct
     8761 gcaccgggat cttcgtgcgg cgagcgagga tctcgtccat caagctcttg aagtcgggag
     8821 aaaggtgctg caccaccacg tacgccatgc cgctcgcgtt cggcacgctg tcgaaaaatc
     8881 gctccaacgc ttcgagccca cctgccgaag ccccgatgcc gacgatgtaa ggcgaagagc
     8941 tcacctcggc gacgagagaa tgcggatctc ccgcg
//
LOCUS       DASTKS010001234         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_644181, whole genome shotgun sequence.
ACCESSION   DASTKS010001234 DASTKS010000000
VERSION     DASTKS010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644181"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /locus_tag="VFQ35_26340"
     CDS             complement(<1..1840)
                     /locus_tag="VFQ35_26340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934260.1"
                     /translation="MREGDLIAGRYRLERRAGTGGMGTVFRAVDVEDGRRVAIKTLSD
                     QALGSSEPSSGAHLAERFEREASVLAVISDAAIVRYISHGVSEWQEPYLVMQWVEGQD
                     LASRLNDRGLSSNESLRLALRLAEALSSLHSNGIVHRDVKPANVMLEHGEVERAVLVD
                     LGVARRAGFASATVTGARVGTPSYMAPEQIRDPRKVDGRADVFSLGCILFECLSGRRA
                     FDAEDGLGAIAKILLERAPSLATLRPDLSSAVSAVVASMLERDPADRPFADAALSAAL
                     SEALAADAEHDSPPPRLALKPAVATRTEVFGTRAEIAEPKVARRSAAPSPARIPYRKG
                     LLVGRERELSEIAGFVASGARCIWLWGAPGIGKTRLALELCAGRVLEGATSPPEFVEL
                     GSARRNDDVLRVFAQALGARMRGAEAAADALGRTLRARGPLICVLDGVDAVSDGVEEL
                     VQQLAALAPEARFVMTSRERPRLRASFALEVLPLSTQGERELLTTASRDLSAAAQLFV
                     QALREHGAAPPHGNEDRVDVERIARAVDGVPLALELVAARVPLLGLSRIATDVARPFA
                     LREALVGDAQAPITSQIRLSFDSLTEAEQSTLLGCAVFARRFAAEAV"
     gene            1890..2495
                     /locus_tag="VFQ35_26345"
     CDS             1890..2495
                     /locus_tag="VFQ35_26345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934261.1"
                     /translation="MGSVARSLFLTLALGATACADGDPEPTHGTFLWVGGVAESDIAF
                     AAVSSTQSATLFFCGGPDSLVRGTHWLPYVAPLSGDFSEESSGVRVRGSVVPALLQGT
                     LEVPGERPRSFSAVGARLDGLAGLYEGIAPCGHIGLIVFPEDAEGTRAQGTCVRTVEG
                     EVQVEQVNPVMPLAALDDRGVAVEVGSDPQPVIVRPVVLAE"
     gene            complement(2537..>3554)
                     /locus_tag="VFQ35_26350"
     CDS             complement(2537..>3554)
                     /locus_tag="VFQ35_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9934262.1"
                     /translation="RVGRSLLWGVADARPFADGVQVDAQGLVRGGHSRRAWSEIELAA
                     KAGTTLCLRGESGAGKELGARAFHEAAGLPGSAPFIAVNCAAVPEGLAERLLFGARRG
                     AYSGAVTDQLGYIQAANGGTLFLDEIAELDSRVQAKLLRVLESREVLPLGATRPERVE
                     LRVCVAAHADLREEVQAERFREDLYYRVGRPEVQIPPLRERIDELAWIAAGEIARVEP
                     TLRASVRFLEACARRPWPGNVRELLGEVRRAAHRALRAGKAELSEEELDPEAGERLAE
                     RPSPDDDDAKPVTLPEDERIEQALSESGGNVTQAARALGVHRNQLRRWLSKRGRGSSA
                     PPEK"
BASE COUNT          581 a         1234 c         1181 g          558 t
ORIGIN      
        1 gaacggcctc cgccgcgaac cgcctcgcaa agacggcaca cccgaggagc gtcgactgct
       61 ctgcctccgt gagcgaatcg aatgacaggc gaatttgcga tgtgatcggc gcctgagcgt
      121 caccgaccag ggcctcgcgc agggcgaagg gccgcgcgac gtcggtcgcg atgcgcgaca
      181 agccgagcag gggcaccctc gccgcgacca actccaacgc gaggggcacg ccatcgactg
      241 cgcgggcaat gcgttcgacg tcgacgcggt cctcgttgcc gtgggggggc gcggcgccgt
      301 gctcacgcag agcttgaacg aagagctgcg ccgccgcaga caggtcgcgg gacgccgtcg
      361 tcaagagctc gcgttcgccc tgcgtcgaga gtggcaaaac ctccaacgcg aatgaggcgc
      421 gtaaccgagg ccgctcacga gaggtcatca cgaagcgagc ctcgggggcg agcgccgcga
      481 gttgctgaac caactcttcc acgccgtccg aaacggcgtc tacgccgtcg agcacgcaga
      541 tgagcggacc gcgggcacgg agggtgcgac cgagcgcgtc ggccgctgct tctgcgccac
      601 gcatgcgtgc gccgagcgct tgcgcgaata ccctgaggac gtcgtcgttg cgacgagcag
      661 agccgagttc gacgaactcg ggcggactcg ttgcgccttc gagcacccgc cccgcgcaga
      721 gctcgagcgc gaggcgcgtt ttgccgatgc cgggcgcgcc ccaaagccag atacagcgag
      781 cgccactcgc gacgaacccc gcgatttcgg acaactcgcg ttcacgccca acgagcagcc
      841 cttttctgta aggtattcgc gctgggctgg gagccgccga gcgccgggcc accttcggtt
      901 cggcaatttc ggctcgggtg ccgaagactt cggttcgcgt cgcgacggcc ggcttgagag
      961 cgagacgcgg cggcggcgag tcgtgctccg catcagccgc caacgcctcc gacaaagcgg
     1021 cagagagcgc ggcgtcggcg aaaggccgat ccgccggatc gcgttctagc atgctcgcga
     1081 cgaccgccga caccgcagac gaaagatcgg gacgcaaggt agcgagcgaa ggcgcgcgct
     1141 cgagcagaat cttcgcgatc gctccgagcc cgtcctccgc gtcgaatgcg cgacgaccgc
     1201 tcaagcactc gaataggatg cacccgagcg agaagacgtc ggcgcggccg tcgaccttgc
     1261 gcggatcgcg aatctgctct ggggccatgt agctcggcgt gcccacccga gctcccgtca
     1321 ccgtcgccga agcgaagccg gcgcgccgag cgacccccaa atctaccaac acggcgcgct
     1381 cgacttcgcc gtgctccaac atcacgttcg ccggcttcac gtcgcggtgt acgatgccgt
     1441 tcgagtgcag cgacgacagc gcttcggcga gacgcagcgc gaggcgcaag gattcgtttg
     1501 agctcagccc gcgatcattc aggcggctcg ccaaatcttg cccttcgacc cactgcatga
     1561 cgaggtaagg ctcttgccac tcgctcacgc catgagaaat gtagcgaacg atcgccgcat
     1621 ccgagatcac ggcgaggacg ctcgcttcgc gctcgaagcg ctcggccagg tgggctccgg
     1681 aagacggctc cgagctcccc agcgcctggt cgctgagggt cttgatcgca acgcgtctcc
     1741 catcttcgac gtccacggca cgaaacaccg ttcccatgcc gccggttccg gcgcgcctct
     1801 cgagccgata gcggccggcg atgaggtcac cttctctcat gtgcgctcga agctacgcga
     1861 acccgtatca ggcgtgtata atgcccgacg tgggttccgt cgctcgcagc ctatttctca
     1921 cgctcgcgct cggtgccacc gcttgtgcgg atggagaccc cgagccgacg cacggcactt
     1981 ttctgtgggt cggcggcgtc gcggaaagcg acatcgcatt cgcagccgtc tcgtccacac
     2041 agtcggcgac gctgtttttc tgcggcgggc cggactcgct cgtgcgcggc acgcactggc
     2101 ttccgtacgt ggcgcctctc agcggcgatt tttccgagga aagttccggg gttcgtgtcc
     2161 ggggcagtgt cgttcccgcg ctgctgcagg gcacgctcga ggtgcctgga gaaaggccgc
     2221 gctcgttttc ggcggtcggc gcacgcttgg acggtctcgc cggcctctat gagggtatcg
     2281 cgccctgcgg gcacattggg ctcatcgtct ttcccgagga cgccgaagga actcgggccc
     2341 aagggacctg cgtccgtacg gtcgagggcg aggtccaggt ggagcaggtg aacccggtga
     2401 tgcccctcgc cgcgctcgac gaccggggcg tagcggtcga ggttggcagc gacccgcaac
     2461 cagtcatcgt tcgcccggtc gttttggccg aatagccacc gcggcgacga acctcacgcg
     2521 acttcgcgcg aggagatcac ttctccggcg gggcactaga gccgcgcccg cgcttgctga
     2581 gccagcgtcg tagctgattg cggtgtacgc cgagagcgcg cgcagcttga gtcacgttgc
     2641 cgccactctc cgagagcgct tgttcgatgc gctcgtcttc gggcaaggtg actggcttcg
     2701 cgtcgtcgtc atcgggcgaa ggtcgctcgg ccaagcgctc gcccgcttcg gggtcgagct
     2761 cctcctcact gagctcggcc tttccagcgc ggagcgcgcg gtgggccgcg cgtcgcactt
     2821 cgcctagaag ctcgcggacg ttgcctggcc agggcctgcg cgcgcacgcc tcgaggaacc
     2881 gaaccgaagc gcgcagtgtc ggctcgacgc gcgcgatttc gcccgcggcg atccaagcga
     2941 gctcgtcgat gcgctcccgg agcggtggga tctgcacttc gggtcgcccc actcggtagt
     3001 acagatcttc gcggaaccgt tccgcttgca cctcttcgcg caagtcggcg tgcgccgcga
     3061 cgcacacgcg aagctcgacg cgctccggtc gcgtcgcgcc gagcggcaac acctcgcgcg
     3121 attcgaggac gcgcaggagc ttcgcttgca ccctcgaatc gagctcggcg atttcgtcca
     3181 agaagagcgt cccgccgttg gcggcttgga tgtagccaag ctggtcggta accgctcccg
     3241 agtaagcccc gcgtcgagcc ccgaacaaga gccgctcggc caggccttct ggcaccgcgg
     3301 cgcagttgac ggcgatgaaa ggcgccgagc caggcagccc tgcggcttcg tggaaagctc
     3361 gagcgccgag ctctttgccg gcgccgctct cgccgcgcaa gcacagcgtg gtgcccgcct
     3421 tcgcggcgag ctcgatttcg ctccaggcgc gccgagagtg accacctcgg acgaggccct
     3481 gagcgtcgac ttgcaccccg tccgcgaagg gacgcgcgtc agcgactccc cacaacaacg
     3541 agcgcccgac ccgc
//
LOCUS       DASTKS010001235         8574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1316139, whole genome shotgun sequence.
ACCESSION   DASTKS010001235 DASTKS010000000
VERSION     DASTKS010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316139"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFQ35_26355"
     CDS             <1..769
                     /locus_tag="VFQ35_26355"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HET9934263.1"
                     /translation="NVVGLVGERGRELNDFVRDSLGPDGLAKSVLVTATSDQPPLVRA
                     RGAESATAIAEYFRAQGKSVLLVMDSVTRYAMALREAALAAGEPPATKGYPPSVFAAL
                     PRLLERAGTGAGAGVITALYTVLVEGDDLSDPIADSVRSILDGHIVLSRSLAEKNHFP
                     AVDVLQSISRLAPAISPEPIQRAGGSVRDLMSAYREALDLIQVGAYVAGSDPRVDAAC
                     VASPAIEQFLKQRTNERTSLEETQARLLKLAMLTQKR"
     gene            807..1265
                     /gene="fliJ"
                     /locus_tag="VFQ35_26360"
     CDS             807..1265
                     /gene="fliJ"
                     /locus_tag="VFQ35_26360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HET9934264.1"
                     /translation="MSQRKKRLKKLIELREHEVEQRLIRLAEQRAGEERAKQALMQQV
                     ELERSAAERRLSAISRAFDVNSLNQETDWLVTCGRRRELAEREMVRARRAVTDAQNQV
                     LKAKNELKKIELLAERVEAEEKVRLERTEQRFSDELAALRFAEEKRKEEP"
     gene            1262..1876
                     /locus_tag="VFQ35_26365"
     CDS             1262..1876
                     /locus_tag="VFQ35_26365"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HET9934265.1"
                     /translation="MSETSQGIGAARDPLLDRARELEERDREELAKRFAIALAPQIIS
                     QLPPPPPKGAAEPPGGWRGSDGVLTPTGASEAAFQATAGAEGDAEPEANQRIVLRVQT
                     RDLGEVSLVLDRSEGAVRVVVGVQDASTVERMLPERDALARQLQGSGLTIQSIQFVRQ
                     SEVGTVLAQSTSVGRARTFATQRQPATEDEKVRRRGSRKLNLIG"
     gene            1881..2570
                     /locus_tag="VFQ35_26370"
     CDS             1881..2570
                     /locus_tag="VFQ35_26370"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HET9934266.1"
                     /translation="MVDSVSSKSSAASAAGLTSALGGDKALGKDAFLKLLVAQLKHQD
                     PLKPQDDSAFVAQLAQFSSLEQTMGINSRLDTLSAQSQGLQNSQVISMVGKEATVKGS
                     LVTSDGSGAGVPVAFTLDGKAASTQIVIKNQAGNTVRTIDVGAKDAGTARITWDGRSD
                     TGIVQPAGTYQITVQAKNDSGAPVSVSQETKGMIESVSFDKGYPVLNLDTGVSVPVSD
                     LLQVESPPNTK"
     gene            2603..3892
                     /locus_tag="VFQ35_26375"
     CDS             2603..3892
                     /locus_tag="VFQ35_26375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HET9934267.1"
                     /translation="MVLRAMYSGVSGLRAEGEALGVVGDNIANVNTVGFKSQRAVFED
                     VLGHSILAGTSSALPGSGVRIGEVQQMFTQGTLSNTGVSTDLALNGDGFFVVKGAVDG
                     VSGNFYTRAGQFVLDKDGFLTNTQGLQVQGYASNGDGTFQASPTSVKAPTSSLAARAT
                     SGITITANLDASSKVPTWDATQPAQTSNFSTSITVYDSLGTAHSVDVYFNKTADNAWD
                     YHAMVNSSELNPPGTGFTEVGTGSLAFTANGALDSVTTTTPIAPAWGGGATGGTPITL
                     NFGTPIASGGTGLDGTTQFASANAVSSQSQDGYTSGDFSGISVDGQGVVMGLYTNGQK
                     LAISQLAVAKFRSNDGLGRAGQNLWIETRESGTAALGTASSGGRAAVSSGALEGSNVD
                     LAEEFVGLIQHQRSFSANSKTITTADEMLQELINIKR"
     gene            3941..4522
                     /locus_tag="VFQ35_26380"
     CDS             3941..4522
                     /locus_tag="VFQ35_26380"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HET9934268.1"
                     /translation="MEQAAVESPRVEKKGKGRMVGILVALVLVLSAGAAGAVFAPRLL
                     SKGQPQDKAHAAEGEHEDAAEAPAEEEKKAEEAEEEEEEAPKKKRTVPAGEIAELTPV
                     ISDIRAQDGTLRHIKVQIAVELPKAEKAEEFKHFIPYAREATIAYLRAQEFDRLSDPK
                     SFDAVRKEIEKEVIGAVGKSHARRVLITDFVVQ"
     gene            4659..5450
                     /locus_tag="VFQ35_26385"
     CDS             4659..5450
                     /locus_tag="VFQ35_26385"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HET9934269.1"
                     /translation="MTRVASNFARAARRSLPFLVKRRARLLPSSVVFVDVSPDARQVE
                     SPVYEVLFEEAGGPARASLFFNTEAVAVTLEGVLGGAGASTAGLGNALSVAQSALVSR
                     VVKQLAEDFVRAVKEEVGVAVRVVSSRSIAAGDERDVNISDGLGVDCAFDGLSNAAKI
                     SLAMGAEALEAAIKDQEPEDPSAGDPRFAEALHEVPVEAVAELGRVSLGLRRLLTLQV
                     GQVLRLNSAIDDPVIVRVAGVPKFSGSPVISRGQLAIQIKARHED"
     gene            5464..5829
                     /gene="fliN"
                     /locus_tag="VFQ35_26390"
     CDS             5464..5829
                     /gene="fliN"
                     /locus_tag="VFQ35_26390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HET9934270.1"
                     /translation="MSDPNGGDKGSGPPKHQSPISAHSLDPSSLAPLRGADSLSFVMD
                     VPVELTIELGRKQVKIGEILKLGPGSVLELDKANGEPLDVYVNNRLVARGEAVVLGER
                     YGIRLIEVLVGDENGASKA"
     gene            5826..6644
                     /locus_tag="VFQ35_26395"
     CDS             5826..6644
                     /locus_tag="VFQ35_26395"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET9934271.1"
                     /translation="MTPRRQAVYVTFGAALIGAALSSASVLAQPWRDPPPPAPSATTA
                     PVEAPAPAPKAAAPASSAPRSYLRQQAATKKPVASAESPSALRVALMGGLVAGLGVWA
                     LLKKRKKQTDLRATRSDLEVLSSARVGSKAQVVVINVGGRKVLLGVTESEVNRLAWLD
                     GELDGDGATTEPFDDAEPFVPAPERKLAPSPTPNKVARVEPRRFRDALLGALGQPQKA
                     PAANGADAALAIAESTQDVVTRSPRVAVPAEAPESMVDVEGQAKGLVLRLQKRA"
     gene            6641..7324
                     /gene="fliP"
                     /locus_tag="VFQ35_26400"
     CDS             6641..7324
                     /gene="fliP"
                     /locus_tag="VFQ35_26400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01103.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET9934272.1"
                     /translation="MSPVSTNALLNGGQLIENGGLAPSLKILLTLTLLALLPAILLAC
                     TSFVRTVVVLSFVRQGIGSMQSPPSQVIIGLSLFLTAFTMAPVADEVKRVALDPYLAG
                     QMTDFQAMEQGVVPLKRFMLRQTREADLKLFYDATDTALPETPDDVPMRLAMPAFVVS
                     ELTTAFHMGVIILLPFLVVDLAVASLLMSMGMMMVPPTTIALPVKLLLFVLVDGWNLV
                     AGSILKSYT"
     gene            7321..7590
                     /locus_tag="VFQ35_26405"
     CDS             7321..7590
                     /locus_tag="VFQ35_26405"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HET9934273.1"
                     /translation="MTVDGAVELFKNAIIVVCMVAGPALIVALIMGLIVGVLQAATQV
                     NEASISFVMKLLAVGATFVVLGSWSVQKLVDYTGRTIASIANVTR"
     gene            7587..8381
                     /locus_tag="VFQ35_26410"
     CDS             7587..8381
                     /locus_tag="VFQ35_26410"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HET9934274.1"
                     /translation="MTPFDFDALIRAEAAAFSLEAVRIAGLIITAPLAWSGAPARVRA
                     ALVVLLTLAVHGQGTLSPELASSASRIAASVGTEFLLGAAMGMIARLSVAAVEVAAEQ
                     IGMAMGLGVAQVFDPQAHGPHNVLGGILRNLALVVALGVGLHRTLLGALIASFRGIPP
                     GSLLSLGLYGETFTSLGSLVFSTGVRLSMPIIAVLLMAQVALAFVSRAAPTMQVFSVG
                     FAVTLGVGAFVLVLSLPDLAQEIAADMSQVQGRIETLLSAALEAPR"
     gene            8378..>8574
                     /locus_tag="VFQ35_26415"
     CDS             8378..>8574
                     /locus_tag="VFQ35_26415"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET9934275.1"
                     /translation="MSESDSDKTEDATPERRRKAREEGQFPRAKDTGAVAGTLAILLV
                     MSAMGPTFWGLLSSFCRRCFS"
BASE COUNT         1549 a         2768 c         2815 g         1442 t
ORIGIN      
        1 gaacgtcgtg gggctggtcg gagagcgtgg tcgcgagctc aacgacttcg tgcgtgattc
       61 gctcggtccg gacgggctcg cgaaatccgt cctcgtgacc gcgaccagcg atcaaccccc
      121 gttggttcgc gcgcgcgggg ccgagtcggc gaccgcaatc gccgagtact tccgcgccca
      181 gggtaagagc gtgcttctcg tgatggactc ggtgacgcgc tacgcgatgg cgctgcgcga
      241 ggcggcgctc gcggccgggg agccgccggc cacgaagggc tatccgccga gcgtgttcgc
      301 ggcgctgccg cgccttttgg agcgtgccgg cacgggcgcg ggtgcgggcg tgatcacggc
      361 cctatacacg gtgttggtcg aaggcgacga cctgagcgat ccgatcgccg actcggtgcg
      421 cagcatcctc gatgggcaca tcgtgctttc gcgttcgctc gccgaaaaga accattttcc
      481 ggccgtcgac gtgctgcaga gcatttctcg tctcgcaccg gcgatttcgc cggagccgat
      541 ccagcgcgcg ggcggaagcg ttcgcgactt gatgtcggcg taccgcgagg cgctcgactt
      601 gattcaggtc ggtgcttacg tcgcggggag cgatccgcgc gtggatgcgg catgtgtagc
      661 cagcccggcg atcgagcagt ttttgaagca gcgcacgaac gagcgtacct cgctcgaaga
      721 gacgcaggca cggctcttga aactcgcgat gctgacccaa aagcgttagc tctctctccg
      781 cgcagcggtg agctcgggcg taaagcatgt cgcagcgcaa gaaacgcctg aaaaagttga
      841 tcgaactgcg cgagcacgag gtcgagcaac gcttgattcg gctcgccgag cagcgcgcgg
      901 gcgaggagcg agcgaaacag gcgttgatgc agcaggtcga gctcgagagg agcgcggccg
      961 aacgccgact gtcggccatt tcgagggcgt tcgacgtcaa tagtttgaac caggagaccg
     1021 actggctcgt gacatgcgga aggcggcgcg agctcgccga gcgcgagatg gtgcgcgcgc
     1081 gtagggccgt caccgacgcg cagaaccagg ttttgaaggc gaagaacgag ctgaagaaga
     1141 tagagctctt ggcggaacgt gtggaagcgg aagagaaggt acgcctcgag cgcaccgaac
     1201 agcgcttctc cgacgagctc gccgcgctcc gcttcgccga agaaaagagg aaggaagagc
     1261 catgagcgaa acgtcgcaag gcatcggcgc ggcccgtgac ccgctgctcg atcgagcgcg
     1321 cgagctagaa gagcgcgacc gagaagagct cgcgaagcgc ttcgcgatag cgctggcgcc
     1381 gcagatcatc tcacaactgc cgccaccgcc gccgaagggc gcggccgagc cgccgggggg
     1441 ttggcgcgga agtgacgggg ttctgacgcc gacgggcgcg agcgaagcgg cgtttcaagc
     1501 caccgcaggc gcggagggcg acgcggagcc ggaggcgaat cagcgcatcg tcttgcgcgt
     1561 acaaacccgc gatttgggcg aagtctcgct ggttttggat cgcagtgagg gagccgtacg
     1621 agtcgtggtc ggcgtacagg atgcgtcaac ggtcgaacgc atgctgccag agcgcgacgc
     1681 gctcgcgcgt caacttcaag gcagcggact cacgattcaa tcgattcaat tcgtccgtca
     1741 aagcgaggtc ggcaccgttc ttgctcaatc cacctccgtt ggccgagctc gcacgttcgc
     1801 gactcaacgc cagcccgcga cggaggacga gaaggttcgt cggcgcggtt ctcgcaagct
     1861 caatctcatc ggctaagcac atggtggatt ccgtaagctc aaagagttcg gccgcgtcgg
     1921 cagcggggct caccagtgcg ctcggcggtg acaaggcgct cggcaaggac gccttcttga
     1981 agctcctcgt cgcgcagctc aagcatcaag atccgctgaa gcctcaagac gactcggcct
     2041 tcgtcgcgca gctcgcgcag ttctcgtcgc tcgagcagac gatgggtatc aatagccggc
     2101 tcgacacgct ttcagcgcag agccagggcc tccagaactc gcaggtcatc agcatggtcg
     2161 gcaaagaagc gaccgtgaaa ggctcgctcg tcacctcgga cgggtcgggc gcaggcgtcc
     2221 ccgtcgcctt tacgttggac ggtaaagcgg cgagcacgca gatcgtcatc aagaaccaag
     2281 ctggcaacac ggtgcgcacg atcgacgtcg gcgcgaaaga cgccggcacc gcccgcatca
     2341 cgtgggacgg acgcagcgat acgggcatcg tgcagcccgc aggtacctat cagatcacgg
     2401 tccaagcaaa gaacgactcc ggtgctcctg tcagcgtctc tcaagagacc aaaggcatga
     2461 tcgagtccgt ttcgttcgat aaaggttatc cggtgctcaa cctcgatacg ggggtgagcg
     2521 tgccggttag cgaccttctc caggtcgaat ctccacccaa caccaagtaa ccgcaacaag
     2581 cagccccatc gggaggatct ccatggttct cagagcaatg tactcaggcg tttctggcct
     2641 acgcgcggag ggtgaagccc tcggcgtggt cggtgacaac atcgccaacg tcaatacagt
     2701 cggtttcaag agccagcgcg ccgtgttcga agacgtgctc ggccactcga ttctcgccgg
     2761 cacctcttcg gccctcccgg gttcgggcgt gcgcatcggt gaagtgcagc agatgttcac
     2821 ccagggcacg ctgtcgaaca ccggcgtctc gaccgacctc gcgctgaacg gcgacggctt
     2881 cttcgtcgtc aagggcgcag tcgacggcgt gagcggcaac ttctacactc gcgccggcca
     2941 gttcgtgctc gacaaggacg gcttcctcac gaacacgcag ggcctccagg tccagggcta
     3001 cgcctcgaac ggcgacggca ccttccaggc ttcgcccacg tcggtgaagg cgccgacctc
     3061 ctcccttgcg gctcgcgcta cgagcggcat cacgatcacg gcaaacctcg acgcttcgtc
     3121 caaggtgccg acctgggatg ccacccaacc cgcacaaact tccaacttct cgacgtcgat
     3181 caccgtctat gactccctcg gcacggccca cagcgtggac gtgtacttca acaagaccgc
     3241 cgacaacgcg tgggactacc acgcgatggt gaactcttcc gagctcaacc cgcccggcac
     3301 cggcttcacc gaggtcggca ctggctcgct cgcgttcacc gcgaacggcg cgctcgacag
     3361 cgtcaccacc acgacgccga tcgccccggc ctggggtggc ggcgcaaccg gcggcacgcc
     3421 gatcacgctg aacttcggca cgcccatcgc ctcgggcggt acgggcctcg acggtacgac
     3481 gcaattcgcc tccgcgaacg cggtctcgag ccagagccag gacggttaca cgtcgggcga
     3541 cttctcgggc atcagcgtcg acggtcaagg cgtcgtcatg ggtctttaca cgaacggtca
     3601 aaagctcgcg atctcgcagc tcgccgtcgc caagttccgc tcgaacgatg gactcggtcg
     3661 cgcaggccaa aacctgtgga tcgagacgcg cgagagcggt acggccgcgc tcggcactgc
     3721 ctcgagcggt ggccgcgcag cggtgtcgtc cggagcgctc gaaggctcca acgtggatct
     3781 cgcagaggag ttcgtggggc tgattcagca ccaacgttct ttctccgcga actccaagac
     3841 catcaccact gcggacgaaa tgctccaaga gctcatcaac atcaaacgtt gatgagcttc
     3901 acggtgcccc gcccataagg tttattacga caggctcgcc atggaacagg ctgcggtcga
     3961 aagtcctcgg gtggagaaga aaggcaaagg ccgtatggtc gggatcctgg tcgctctggt
     4021 actcgtcttg agtgccggag cggctggggc cgtgtttgcc ccacgactcc tctccaaagg
     4081 acagccccaa gacaaggccc acgcggccga aggcgagcac gaagatgccg ccgaggctcc
     4141 cgccgaagaa gaaaagaagg cggaggaagc cgaagaagag gaagaggaag cgcccaaaaa
     4201 gaagcgcact gttcccgccg gcgaaattgc ggagctcact ccggtgatct cggacatccg
     4261 cgctcaagac ggtaccttgc gccacatcaa ggtgcagatc gcggtcgagc tgccgaaagc
     4321 cgagaaggcc gaagagttca agcatttcat cccttacgcg cgagaggcga cgatcgccta
     4381 cctgcgcgca caagaattcg atcggctgtc ggacccgaag tcgttcgacg ccgttcgcaa
     4441 agaaatcgaa aaagaagtca tcggcgcagt cggaaaatcg cacgctcggc gtgttctcat
     4501 caccgatttc gtcgttcaat aggaaggacg cccccagcgt gggccggacc aacactgcgc
     4561 gcgaggcaac gcccgaacaa cccgtggaag cgcctcgaaa tctcaagcga ctcgacctga
     4621 ctggtcgtga acgacgctta cgcggtgcga tgcaagcgat gacgcgagtt gcgtccaatt
     4681 tcgcgcgcgc cgctcgtcgc agtctgccct tcctggtgaa gcgccgtgcc cggctgctgc
     4741 cctcgtcggt ggtgttcgtg gacgtgagtc ccgacgcgcg acaggtcgag agccccgtgt
     4801 acgaggtgct gttcgaagag gcgggcggcc cagcgcgagc cagcctgttt ttcaataccg
     4861 aagccgtcgc cgtcacgctc gagggcgttt tgggcggcgc aggcgcatcg acggcagggc
     4921 tcggcaacgc gctctcggtt gcgcagtcgg ccttggtttc tcgggtcgta aagcaactcg
     4981 cagaagattt cgtgcgcgcc gtgaaggaag aggtcggcgt cgcggttcgc gtcgtttcgt
     5041 cgcgtagcat cgcggccgga gacgagcgcg acgtgaacat cagtgacggg ctcggggtgg
     5101 actgcgcctt cgacggtctg tccaacgccg cgaagatctc actcgccatg ggcgcagagg
     5161 cgctcgaggc ggccatcaaa gaccaggagc ccgaggaccc gtccgcgggc gatccgcgct
     5221 tcgcagaggc actgcacgag gtgccggtcg aggcggtggc ggagcttgga cgagtgtcgc
     5281 tcggattacg tcgcctactc acgctgcagg tgggacaagt gctgcgcctg aacagcgcga
     5341 tcgacgatcc ggtgattgta cgtgtcgcgg gcgtaccgaa gttcagcgga tctcccgtga
     5401 tctcgcgcgg acagctcgcg attcagatca aagcgcggca cgaagattga atcattcggg
     5461 ggtatgagcg acccgaacgg cggagacaag ggctccggcc ctcccaaaca tcagtctccc
     5521 atctctgcac actctctcga tccatcgtcg cttgcgcccc tgcgcggcgc cgattcgctg
     5581 tcattcgtga tggacgtgcc ggtcgagctc accatcgagc ttggccgaaa gcaggtgaaa
     5641 atcggggaga tcctgaagct tggccccggt tccgtactcg agctcgacaa agccaacggc
     5701 gagccgctcg acgtttacgt caacaatcgg ctcgtggcgc gcggtgaagc ggtcgtgctc
     5761 ggcgagcgtt acggtatccg cctcatcgag gtgctcgtcg gcgacgagaa cggagcgtcg
     5821 aaagcatgac accccggcgt caggctgttt acgtgacgtt cggcgcggcg ctcatcggcg
     5881 cggcgctctc gagcgcgagc gtgctcgctc agccgtggcg tgacccgccg ccgcccgcgc
     5941 cgagcgcgac caccgcgccc gtcgaagcgc cggcgcccgc gccgaaagcc gctgctccgg
     6001 cgagctcggc gccgcgctcc tacctcagac aacaggcggc gaccaagaag cccgtcgcgt
     6061 ccgccgagag tccgagcgcc ctgcgcgttg ccctgatggg aggcctcgtc gccggcctcg
     6121 gcgtctgggc gctcctgaag aagcgtaaga agcagaccga cctgcgcgcg actcgctcgg
     6181 atctcgaagt tttgagcagc gctcgcgtgg gcagcaaagc ccaagtcgtc gtcatcaacg
     6241 tcggcgggcg caaggtgctc ctcggcgtca ccgagagcga agtgaaccgc ctcgcgtggc
     6301 tcgacggaga gctcgacggc gacggcgcca ccaccgagcc cttcgacgac gcggagccgt
     6361 tcgtcccggc tcccgagcgc aagctcgcgc cgagcccaac accgaacaag gtcgcgcggg
     6421 tcgagccgcg gcgcttccgc gacgctctgc tcggcgcgct cggtcagccc caaaaggctc
     6481 ccgcggcgaa cggcgccgac gccgcgctcg cgatcgccga gtcgacgcaa gacgtcgtca
     6541 cgcgctcgcc gcgcgtggcc gtccccgccg aagcaccaga gtccatggtc gacgtcgaag
     6601 gccaggccaa aggtctcgtc ctccgcctgc agaagcgcgc atgagccccg tctccaccaa
     6661 cgcgctcctc aacggcgggc agctcatcga gaacggcggg ctcgctccgt cgctcaagat
     6721 cctgctcacg ctgacgctgc tggcgctctt gccggcgatc ctgctcgcgt gcacgtcgtt
     6781 cgtgcgcacg gtggtggtgc tctcgttcgt gcgtcagggc atcggctcga tgcagagccc
     6841 gccgagccag gtgatcatcg ggctttcgct gtttctcacc gctttcacga tggcgcccgt
     6901 agcggacgag gtgaagcgcg tcgcgctcga cccgtacctc gccggacaaa tgactgactt
     6961 tcaggcgatg gagcaggggg tcgttccgct caagcgcttc atgctgcgac agacgcgcga
     7021 agcggatttg aagctcttct acgatgcgac cgacaccgcg ctgccggaga ctccggacga
     7081 cgtgccgatg cgactcgcga tgcccgcgtt cgtggtctcc gagctcacca ccgccttcca
     7141 catgggcgtc atcatcctgc tgccgttctt ggtggtggac ctcgccgtcg cctccctgct
     7201 gatgagcatg ggtatgatga tggtgccgcc gacgacgatc gcgttacccg tgaaactcct
     7261 tttgttcgtg ctggtcgatg gctggaacct cgtcgccggc tcgattttga agagctacac
     7321 atgaccgtcg atggcgccgt cgagttgttc aaaaacgcga tcatcgtagt ttgtatggtg
     7381 gcggggccgg cgctcatcgt cgcgctcatc atgggcctca tcgtcggtgt gctgcaggcc
     7441 gcgacgcagg tgaacgaagc gagcatcagc ttcgtgatga agctcctcgc agtgggagcc
     7501 acgttcgtgg tgctgggctc gtggtccgtg cagaagctcg tcgattacac cggccgcacc
     7561 atcgccagta tcgccaacgt gacgcggtga cccccttcga tttcgacgca ctgatccgag
     7621 ccgaggcggc ggcgttctcg ctcgaggcag tgcgcatcgc ggggctcatc atcacggcgc
     7681 cgctcgcgtg gtccggcgcc ccggcgcgcg tgcgcgcagc gctggtagtg cttttgaccc
     7741 tcgcggtgca tgggcagggg acgctgtcgc ccgagctcgc gtcgtcggcg tcgcgcatcg
     7801 cggcgtcggt gggcaccgag ttcttgctcg gcgcggccat gggcatgatc gctcggctca
     7861 gtgtcgccgc ggtcgaggtc gcggccgagc aaatcggcat ggcgatgggc ctcggagtcg
     7921 cgcaggtgtt cgatccgcaa gcgcacggcc cgcacaacgt gctcggcggc atcttgagaa
     7981 atctcgcgct cgtggtcgcg ctcggcgtcg ggctgcaccg gaccttgctc ggcgcgctca
     8041 tcgcgagctt ccgcgggatt ccgcccgggt cgctgctgag cctcgggctc tacggcgaga
     8101 cgttcacgag cctcggtagc ctggtatttt cgaccggggt gcggctgtcg atgcccatca
     8161 tcgccgtcct cctgatggcg caagtagcgc tcgctttcgt gtcgcgcgcc gccccgacga
     8221 tgcaggtgtt ctcggtcggc ttcgcggtta cgttgggagt cggcgctttc gtgctggtgc
     8281 tctcgctgcc cgacctcgct caagagatcg cggctgacat gtcgcaagtc cagggccgca
     8341 tcgaaacgct gctcagcgcg gcgctggagg caccccggtg agcgagtccg attcggacaa
     8401 aacagaagac gcgaccccag aacggcggcg caaagcgcgc gaagaaggcc agtttccgag
     8461 ggcaaaagac accggcgcag tcgcgggaac gctggcgatt ttgctcgtga tgtcggcgat
     8521 gggcccaacc ttttgggggc ttttgagctc gttttgtcgg cgctgctttt ccga
//
LOCUS       DASTKS010001236        12210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_420571, whole genome shotgun sequence.
ACCESSION   DASTKS010001236 DASTKS010000000
VERSION     DASTKS010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420571"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFQ35_26420"
     CDS             <1..474
                     /locus_tag="VFQ35_26420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934276.1"
                     /translation="FEKMLNEVEANYCVDTSKVFVGGFSSSAWFAATLSCSHGDRINA
                     IGMAAGGEQPELPTCTGPTALALWAGTEDTGNPIDAPSNVTWKGSGAVRDRLVTTNGC
                     SRTTTDWDPRWPTCRAYDDCGKNPVVFCPHPGGHDAGGDTKINSEGFWKLFKSTW"
     gene            complement(505..1065)
                     /locus_tag="VFQ35_26425"
     CDS             complement(505..1065)
                     /locus_tag="VFQ35_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934277.1"
                     /translation="MGGEAGTSTPSFGGAVGTGGVGGEGGVGGTATMGGVAGSATGGD
                     SASGAGAGPISCEASFTTDTNDKGIGAGCAQINPPIEVAACPGKFSIARVLSDRWPKA
                     ENGQLEFGFCTKHCEIDSDCGTGFGCCEPRKGAFCLPYVENVLLPTGCTEPCATNHLN
                     CGEGEICCERLGKVCVSDKCSGICPE"
     gene            complement(1338..2402)
                     /locus_tag="VFQ35_26430"
     CDS             complement(1338..2402)
                     /locus_tag="VFQ35_26430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934278.1"
                     /translation="MPRRLFELFVLGVASLQACSLLAPSDEELLGGGRGSAGAGGASS
                     GGKASVAGAGGAATGGKAGAEGGRSGSGGAGGTSASGADAGGAAGFFGGAGASGAGEE
                     PCSSGRHRCENACVSNSAVESCGAACTPCPTPHNGHATCDGNACGVSCDVGFTACGLL
                     CADLRTDHENCGGCGSDFVCSADEVCQAGSCVSVSGCSDGTREGFAVAGSYLNIAGCA
                     ARWPLGSMRAEKTGIPCGEGLADCAVPADACGPGWHVCAAPPYGPAEVRTRATVAECL
                     AQPGAYAAAVGDQACDPCSPDDTGAGAACCGRNCVQQNGSCIYPGMTAWFGVINGHTN
                     VCGDIESNSSVRGVLCCLDL"
     gene            complement(2444..3997)
                     /locus_tag="VFQ35_26435"
     CDS             complement(2444..3997)
                     /locus_tag="VFQ35_26435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934279.1"
                     /translation="MSSSALRVVGRYQVGPTIGSGGMATVHLGRLFAPAGFSRTVAIK
                     QLRAMHSSDADLSAMLLQEAKLAARIRHPNVVAPLDVVVMGDETFIVMEYVHGESLAG
                     VMRRLAGAPVPVEIAAAIMGQVLLGLHAAHRATSEDGQPLGLVHRDVSPHNIMLASDG
                     MARLVDFGVAKAELRAVSTQRGLLKGKLGYIAPEQVAMETVDHRTDLFAAGVVLWEML
                     TGQHLFAGGTPNTAIEKLSKLEVPAPSELVPGLPVALDRIVAKALARDPADRFADAHA
                     MAVELAAAVPAASPIELAGWLDRVAGDSLAKRAQQVAELESLVLDDVTVDLPLTPPVV
                     TAPAPAAAASKPAVLPRSTPERKSVAPKSTSHAKRDRAVMAAAFGVALAALGIASFGL
                     VHPRESAEPRVSIQPLTPVPEKVSVQALPPVSEKAPAETPMPEPSQLLKASSALAETE
                     PLEAPAPSESARASKTKPAPLPNRRPQSSGNDAPLPNAARSEKPRGACAMPYTIDANG
                     VKRFNPECF"
     gene            4261..5652
                     /locus_tag="VFQ35_26440"
     CDS             4261..5652
                     /locus_tag="VFQ35_26440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9934280.1"
                     /translation="MPRVLRYAARDNDQPLWCSRMTSNSTRAQQPTRVVKYAKTQIVV
                     VEGPDSGKELEVAGTPVRIGTSADNTLVLNDEFVSRRHCELLPTPAGVRVRDDGSRNG
                     MFVGATKVADATYTEPFQLRLGQTVLAITPLTATVDREQSTTDRFGDILGGSACMREL
                     FADLMRIAPTDVSVLIEGETGTGKELVADSIHRASPRANGPYVVFDCSAVAANLAESE
                     LFGHERGAFTGAVAARAGVFEQAEGGTIFLDELGELPKDLQPKLLRVLEKREVRRVGG
                     NKTIPVDVRVIAATNRNLPAEIERGNFREDVYFRIATTHVYVPALRDRLDDLPVLVGH
                     FLSRAVPPRTIDDIPPHVWEMLSSYRWPGNVRELQNAVQRLLITPERALNPAAQRAAT
                     SPAPAAPAAPVEQPPAKVEPLRVARREAADEFERNYLKLVLDKTDGNVTRAAAIAEVS
                     RQMIQHLMRKHGM"
     gene            5801..6484
                     /locus_tag="VFQ35_26445"
     CDS             5801..6484
                     /locus_tag="VFQ35_26445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9934281.1"
                     /translation="MREPMNFGRLQSRAPAMRRLFEALAEIALTNANLLVEGETGVGK
                     ELVVEEVHRRSARAAAPLVTFDCAALSSEFAQRELLGVAAGEPTDEGSLAQAQGGTLV
                     LDHVEALAVELQAALHDALKFQDVRVIALTTATLVHDVERGNFHRELYQRVAAHRLEI
                     PPLRERMEDIPLLVEALLIEDGARLSPVDVPDELWEHFRRQCWRGNVRELKNALSRWY
                     VMKRAVPRA"
     gene            6635..7321
                     /locus_tag="VFQ35_26450"
     CDS             6635..7321
                     /locus_tag="VFQ35_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934282.1"
                     /translation="MNPADQKMFVPEGVEFESTNSFSADELLKIETFTLRTYGKNAMV
                     LASLRNVGRQLACDNHVAMVLQGSNGETFASADVYMYAAALYEDFTTAGCLAPGEVGV
                     GGAILLPEGGAEPDSIVSQVSHVVYQFQGYLPTDAVKLDLVGLADVTISSAASRVAKV
                     QGRFHNGGTTPISELSAFAFSLNAAGRPLAMGRTQNSSATMPTSDWNFDISLEGPVDK
                     YVVVASYPTQ"
     gene            complement(7324..8562)
                     /locus_tag="VFQ35_26455"
     CDS             complement(7324..8562)
                     /locus_tag="VFQ35_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934283.1"
                     /translation="MDFSIIIAVAVVVVVLILTSLIVTTFLRSVDAGEILLASFGKNL
                     RIYRGPCKALVVPVVTQLRVIPAQAINVDIEITDQTADLDANGRSAPVKVTVRASAIV
                     SVGENNAMVNTAANKFFSKSSNEQLSTLTDVLSSAGRRAINLLKHDQLFNARSAAPAT
                     STALAPPSTNSLAVARGEDDELAVIIKDACSRELMDLGLSFNSLNIKAVLSEVAEARR
                     RESAARAKADADVVQAQEEQRARIAQLEAAQKINDQERALNMQIASNGAAIARAEAEK
                     QAAVREQREAELGASQITQARADAEQNVIQQEAEGRAQAARIRAVAEAESEAIRLKAQ
                     ALLASGGAYLDLRRLEMAPELTREIANALGESQFVNFGASGGGSGERGAVTTASDDVL
                     RVVQTLMAAQVITGKSPKDS"
     gene            complement(8590..9273)
                     /locus_tag="VFQ35_26460"
     CDS             complement(8590..9273)
                     /locus_tag="VFQ35_26460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934284.1"
                     /translation="MFLLLMVAGLVGLTIMAIPAFGRHGHGHAAHAHSHGWGQARGGA
                     LRLLKGGRVHAPRALPRHAPAAAGAREHGEILVDAAPGQRFNWLPSPRAIFSLLALYG
                     AFGNALVRAFHLSPTFAALGALLLALLVERLCVKPLFSLLFRFQAPPSSPLEALIHTE
                     ARAVVPFRNGRGMVSVELDGRLIQLVARLRDDQAGLPVAVGDTVRVEDIDATLETVTV
                     AFIFDPRSP"
     gene            complement(9340..10257)
                     /locus_tag="VFQ35_26465"
     CDS             complement(9340..10257)
                     /locus_tag="VFQ35_26465"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934285.1"
                     /translation="MSDSPKNLIDPNSVSGSLEQREAQARPLRDEARKALERGELELA
                     MLRASDALMLLPNSRATLDLVDSIARGSSDPLTLVPLSVSVALTACRTRVLAMNHEVL
                     DAIALLRHVIIAAPSLECMVWVRDWLSPSAIRQLGLRELLSPLVAFVKLWTELPLRLD
                     PGDARYPNVRATADVLARVRAEFPAEAPLYVAELVLLRRLGDAQAALALARSAVGRFP
                     EDWAVLVTVQNVERDAKNPDAALSYARRALQVLPRDGSPLHDAAWAYWEAKRPSDALA
                     LFEELLEEFPDYPLSADGRALVARLAAQN"
     gene            complement(10268..>12210)
                     /locus_tag="VFQ35_26470"
     CDS             complement(10268..>12210)
                     /locus_tag="VFQ35_26470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934286.1"
                     /translation="YIPVLFCFLAFWQLGELLVAQMVVARASSAAGRAAVVVLPDDPA
                     FYSGEAKGSLGGIREGSVRMAAGMILAASPHLAEEFYVNLSNPPEEVDAIHATVTATF
                     RCNRLRFVCGADGLVHLSATSSHAYHGAKYEYAPTDLSNVTSSNTSTSTDASCFDGGP
                     SKNGSGGKGGNDTGNGGRSGTGGRDSSNGSGGRSATGGADGGCPPGQSKNPDGSCSST
                     RGGPDGSCKPGYVENPDGTCSSAECAKIKDPKRRKTCDVNGPCPNPNDERDEVGECCK
                     ALKTVTLSDGSKAKRCNEEKLACTNQESATGDSGHKLTGHIIADDTVLSLPEIEPKFT
                     EICGDKTSCSATSNGDFVKDIGVKIVANMKLKEKAVWCPKDPSGKRKAEIEEIKQEWN
                     DWVKTYKEQVIDEYLQANPGAKRKDAESKAWKAKKAEADKIRADNVKSGTRPRAGQSG
                     VKSYPGPDPALGDFFSALDSWCSELEKVGDKGPYEKYLAPNVKAAEDRMLAAYSSLPS
                     TIEQSIDTSLQLQSGINHTERKVIELLEGWANKGGKDLKSMKLRMDLEGDWSPCASCT
                     RALQEFVATYGGSVSYCWTKGIYLSGIARTKEFVVAGGSLMAGTIRHKGCVTITPTGT
                     NFAGERLCDKGETRGVPSAAKK"
BASE COUNT         2161 a         4109 c         3808 g         2132 t
ORIGIN      
        1 ttcgagaaga tgctgaacga ggtcgaggca aactactgcg tcgacacgag caaggtcttc
       61 gtcgggggtt tcagcagcag cgcctggttc gcggcgacgc tgtcgtgctc gcacggcgat
      121 cgcatcaacg cgatcggcat ggcggcaggc ggtgagcagc cagagctccc cacctgcaca
      181 ggccctactg ctctcgcttt gtgggctggc actgaagaca ccggcaaccc gatcgatgcg
      241 ccttcgaacg tcacgtggaa aggctcaggg gccgtgcgcg atcgattggt aacgaccaac
      301 ggctgcagtc gaaccaccac cgattgggac ccgcgttggc cgacttgccg ggcctacgac
      361 gattgcggca agaacccggt cgtcttctgc ccgcaccccg ggggacacga cgccggcggc
      421 gacaccaaaa tcaactcgga gggattctgg aagctcttca agagcacctg gtgatcccca
      481 aacccgaaag cgactgcgga ccgcctattc aggacaaatc ccactgcact tgtcggagac
      541 gcacactttg ccgagccgtt cgcaacagat ctctccctcg ccgcagttca ggtgattcgt
      601 cgcgcaaggt tcggtgcaac cggtgggaag caagacattc tccacgtagg gcaggcaaaa
      661 cgcgcctttg cgcggctcgc agcagccgaa gccagtgccg caatcagagt cgatctcgca
      721 atgcttggta cagaacccga actcgagctg cccattctcg gctttgggcc agcggtcact
      781 caacactcga gcgatcgaaa acttccccgg gcaggcggct acttcgatgg gtggattgat
      841 ttgggcgcag cccgcgccga tccccttgtc attcgtatcc gtagtgaacg acgcctcgca
      901 cgagatcggg cccgcgccgg cgcccgacgc agagtcaccg cccgtggcgc tgccagcgac
      961 gccgcccatc gttgcagtgc cgccaacgcc gccctcgccg ccgacacctc ccgtccccac
     1021 cgctccgccg aagctcggcg tcgaagtgcc ggcctcgcca cccattccgc cgctcgcctc
     1081 gccactcgcc cccgcgcgag cccctgctgt tcctgcgctc ccgccgctac tccgcttccc
     1141 gccagagccg ctgctcgcac cggccgcgcc gccactgcca acagcgtttt tttcgtcctc
     1201 cccgcagccg ctgaaacaga ccacgccgca aaccacgatt gcccatacac gccgcatgta
     1261 agaagcatac atcgagtcgc gctcggaaac agagagtgat tgtaacctgc gaaccgaagc
     1321 ctaatcttgc agcgggatca gaggtccaaa cagcacaaaa cgccgcgaac gctgctgttc
     1381 gattcgatgt caccgcagac gttcgtatgg ccgttgatca caccgaacca cgccgtcatc
     1441 cccggataga tacagctacc attctgttgc acgcagtttc ggccgcagca ggccgcgccc
     1501 gctccggtgt cgtccgggga acacggatcg caggcttggt ctcctaccgc cgctgcgtag
     1561 gctcctggtt gcgccaggca ttcggcgacc gtcgcgcgcg ttctgacttc ggctgggccg
     1621 tacggcggcg cggcacaaac gtgccagccg gggccacacg catccgcggg gacggcgcaa
     1681 tcagcgaggc cctctccaca cggaatgccc gttttctccg cgcgcatcga gccgagcggc
     1741 caacgcgcgg cgcacccagc gatatttaga tacgaccccg ctaccgcaaa tccttcgcgc
     1801 gtgccgtcgc tgcaaccgct cacggaaacg cagctcccgg cttggcaaac ttcgtccgcc
     1861 gagcagacga agtcgctccc gcagccgccg cagttctcgt gatccgtccg caggtcggcg
     1921 cacagcaacc cgcacgccgt gaagcccaca tcgcaactta cgccgcacgc atttccgtcg
     1981 cacgtcgcgt gaccattgtg cggagttggg cacggcgtgc acgcggcgcc gcagctctcg
     2041 acggctgagt tgctgacgca cgcattctcg cagcgatggc gcccactgct gcatggctcc
     2101 tcaccagcac ccgatgcgcc cgcgccgccg aagaacccag ccgctccgcc ggcgtcggca
     2161 ccggaagccg aagtgccgcc ggctccgccc gaacccgagc gccctccctc ggcacctgct
     2221 ttcccgccgg tcgcggcgcc gccggcacca gcgacgctgg ccttcccgcc gctcgacgct
     2281 ccgcctgcac cggcactgcc gcgcccgccg ccgagcaact cctcgtcgct gggagcgaga
     2341 agagagcatg cctgaaggct cgccactccg aggacgaaga gctcgaacaa gcgacgtggc
     2401 atggcaaccc gaagggggcg cactgtcccc cgcgaccgct gcatcaaaag cactccgggt
     2461 tgaatcgctt cacgccgttc gcgtcgattg tgtaaggcat ggcgcaggct ccgcgcggct
     2521 tctcgctgcg cgcggcattc ggtaagggcg cgtcatttcc actcgattgt ggccggcggt
     2581 ttggcagggg cgccggtttc gtcttgctgg cgcgcgcact ctcgctaggc gcgggagcct
     2641 cgagcggctc tgtttcggcc agcgcagacg aagctttcaa aagctgactc ggctcgggca
     2701 ttggcgtctc ggccggcgct ttctcggaca ccggcggcaa ggcttgcacg gataccttct
     2761 ccggcacagg cgtcaaaggt tggatggaca cgcgcggctc tgcgctttcg cgtggatgca
     2821 cgaggccgaa cgaagcgatg ccgagggccg cgagcgcgac cccgaaagcc gctgccatga
     2881 cggctcggtc ccgcttcgcg tgagatgtag acttgggcgc aacgctcttt cgttcaggcg
     2941 tggagcgggg cagaactgcc ggcttcgacg ccgcagcggc cggcgctggc gcggtcacga
     3001 cgggaggcgt cagcggtagg tcgaccgtga cgtcatcgag taccaacgac tcgagctcgg
     3061 cgacctgctg agcgcgcttg gccaacgagt cccctgcgac ccgatcgagc catcccgcga
     3121 gctcgatggg gctcgcggcg ggcacggcgg ctgcgagctc gaccgccatg gcatgagcgt
     3181 cggcgaagcg atccgccggg tcgcgtgcca acgcctttgc aacgatgcga tcgagcgcga
     3241 ccggcagccc cggcacgagc tcgctcggag cgggtacctc gagcttcgaa agcttctcga
     3301 tcgcggtgtt cggcgtgcct ccggcaaaca ggtgctgacc cgtgagcatc tcccaaagaa
     3361 cgaccccggc cgcaaacaag tccgtccgat ggtcgacggt ctccatcgct acctgctcgg
     3421 gagcaatgta gccgagcttc cccttcaaca agccccgctg ggtggagacg gcgcggagct
     3481 ccgccttggc cacgccaaag tcgaccaaac gagccatgcc gtccgaagcc agcatgatgt
     3541 tgtggggaga gacgtcgcgg tgaaccaacc cgagcggctg cccatcctca ctcgtggcgc
     3601 gatgggcggc gtgcaagccg agcagcacct gccccatgat ggcagcggca atttccacgg
     3661 gtaccggagc acccgcgagg cgacgcatca cgcccgccag cgactctccg tgcacgtact
     3721 ccatcacgat gaacgtctcg tcgcccatga cgacgacgtc gagcggcgcg accacgttcg
     3781 gatggcggat gcgcgccgcg agcttcgcct cttggagcag catcgcgctg aggtcggcgt
     3841 cggacgagtg catcgcgcgc agctgcttga ttgcaaccgt ccgggaaaac ccggccggag
     3901 cgaagagccg ccccaggtgc acggtggcca tcccaccgga accgatcgtc ggtccgacct
     3961 gataacggcc aacgacgcgc agcgcggacg agctcatcga caggctccca ctgtgctttc
     4021 gttcatctcg ccgtcccgcg ccacttcgaa ggaggccgga ctgaaacgcg actggtggaa
     4081 atcgtcattt cgagcgctga agactgtacc acccggcgcg tgaacggctt tggatttgtc
     4141 gcgtcggttc catcgaccca tccggcccgc ttgccgggca ttttccgtcg caagtgccgt
     4201 agccgccgcc ctaccgcgca gcgctctcga ttgcgcgcgc gtccacggag ggagcctcgc
     4261 atgccccggg tgttaaggta cgcggcacgt gacaacgatc agccactttg gtgctcgcgc
     4321 atgaccagca actcgactcg tgcgcagcaa ccaactcgcg tcgtcaaata cgccaaaacg
     4381 cagattgtcg tggtcgaagg ccccgattcg gggaaagagc tcgaggtcgc tggtacgccc
     4441 gtgcgcatcg gcacgtcagc cgacaatacg ctcgtcttga acgacgagtt cgtctctcgg
     4501 cgtcactgcg aactcctacc cactccggcg ggtgtacgag ttcgcgacga cggctcgcgc
     4561 aacggtatgt tcgttggggc caccaaggtc gcggacgcga cgtacaccga gcctttccag
     4621 ctccggttgg gccaaacggt gcttgcgatc actccgctca cggcaacggt cgaccgcgag
     4681 caaagcacga cggatcgctt cggtgacatc ttggggggct ccgcgtgcat gcgcgagctc
     4741 ttcgcggatc tgatgcgtat cgctccgacc gacgtgagcg tgctcatcga gggcgagacc
     4801 ggaaccggca aagagttggt ggccgactcc atccaccgag cgagcccgcg cgcgaatggg
     4861 ccctacgtgg tattcgattg cagcgcggtc gcggcgaacc tggccgagag cgagctgttc
     4921 gggcacgagc gtggagcgtt cacgggcgcg gtcgcggcgc gcgcgggcgt cttcgaacag
     4981 gccgagggtg gcaccatttt cctcgatgag ctcggggagc tacccaaaga ccttcaaccc
     5041 aagctcctgc gcgtgctcga gaagcgcgag gtccggcgcg tcggcggcaa caagaccatc
     5101 cccgtcgacg tgcgcgtcat cgccgccacc aatcgcaacc tgcctgccga aatcgagcgc
     5161 ggtaacttcc gcgaggacgt ctactttcgc atcgcgacga cgcacgttta cgtgccggcg
     5221 ctccgcgacc gcctcgacga cttgccagtc ctcgtgggtc acttcttgag cagggcggtc
     5281 ccgccgcgaa cgatcgacga catccctccc cacgtttggg aaatgctgag ctcgtatcgt
     5341 tggccgggca acgtgcgcga actacagaat gcggtccagc gcctgttgat caccccggag
     5401 cgtgccctca atcccgccgc ccagcgcgcc gcgacgtctc cggccccggc ggcacccgcc
     5461 gcaccggtag agcagccacc ggccaaggta gagccgctgc gcgtcgctcg gcgcgaggca
     5521 gccgacgagt tcgaacgcaa ctacctgaaa ctcgtgctcg acaaaaccga cggcaatgtg
     5581 acgcgcgccg ccgcgatcgc cgaggtttcg cgtcaaatga ttcagcattt gatgcgcaag
     5641 cacggcatgt aaaccgcccg caagcgcgcg gtgccaaacg ccgcttggca gcgcgcaatg
     5701 cgtagttgct tcggctcgag agcccgcgcg atgcttcgag cagcgctccg agctcgtacg
     5761 tcctctcagg tggctcgaaa tttgtatgcg ctcgcgccgc atgcgtgagc ccatgaactt
     5821 cggccgtttg cagtcacgag ctccggccat gcggcggctg ttcgaggccc tcgccgaaat
     5881 cgcactcacg aacgcgaacc tactggtcga gggcgaaacc ggcgtcggca aagagctcgt
     5941 cgtcgaggaa gtgcatcgca gaagcgcgcg cgcggcggct ccgctcgtaa cgttcgactg
     6001 tgccgcgctg tcgtccgagt ttgcccagcg cgagctgctc ggggtagctg ccggcgagcc
     6061 gactgacgaa ggttctctgg cccaagccca aggcggcacg ttggtgctcg accacgtcga
     6121 agccttggct gtggagctcc aggcagcttt gcacgacgca ctcaagtttc aagacgttcg
     6181 cgtcattgcc ctcacgactg cgaccctcgt gcacgacgtc gaacgcggca actttcaccg
     6241 tgagctctac caacgcgtcg ccgcccaccg cctcgagatc ccgcccttgc gcgagcggat
     6301 ggaggacatc cccctcctcg tcgaagccct cctcatcgag gacggcgctc gcctgagccc
     6361 cgtcgacgtg cccgatgagc tctgggaaca ttttcgaaga caatgctggc gcggcaatgt
     6421 tcgagaactc aaaaacgcgc tctcgcgatg gtacgtgatg aaacgagcag ttccgcgcgc
     6481 gtgacgtgaa ctttcgcgac aatgcgtgtt aacgttgcgg cgcatgaact cagtgcgcaa
     6541 cgacacattt gtgagagccc aggtcggctc tctactgctc gcggtagcgg cgggatgtag
     6601 cgacgggaaa tccgacccta aagcggactc cgcgatgaac ccggcggacc agaaaatgtt
     6661 cgttcccgaa ggcgtcgagt tcgaatcaac gaactcgttt tcagccgacg agcttctcaa
     6721 gatcgagaca ttcacgctgc gaacctacgg gaagaatgcg atggtgctcg cttccttgcg
     6781 caatgttgga aggcagctcg cctgcgacaa tcacgtcgcg atggtcttgc aggggagcaa
     6841 cggggagact ttcgcgagcg cagacgtata catgtacgcg gcggcgctct acgaggactt
     6901 caccacggca ggctgtttgg cacctggcga ggtcggcgtc ggcggcgcga tactcctacc
     6961 ggaaggcgga gcagagcccg attcgatcgt ctcgcaagtg agccacgtcg tctaccaatt
     7021 ccagggctat ttgccgaccg acgcggtcaa gctcgacctg gtcggacttg cggacgttac
     7081 catctcgtcc gccgcctcga gggttgccaa ggtgcaggga cgctttcaca acggcggaac
     7141 gacgccgata tccgaactca gcgccttcgc tttttcactc aacgcggcgg ggcggccgct
     7201 cgcaatgggt cgcactcaga actccagcgc cacgatgccg acaagcgact ggaacttcga
     7261 catatcgctc gaaggtccag tcgacaaata cgtcgtcgtc gcgtcgtatc ctacccaatg
     7321 agctcaggag tctttgggcg actttccggt aatcacctgc gcggccatca gcgtttgtac
     7381 gacgcgtagt acgtcgtccg aagcggtcgt cacggcgccg cgctccccgg aaccgccgcc
     7441 gctcgccccg aagttgacga actgactttc gccgagggcg ttcgcgatct cgcgggtgag
     7501 ctctggcgcc atttcgaggc gacgcaagtc gaggtaagcg ccgcccgaag cgagcaacgc
     7561 ctgggccttg aggcgaatgg cctctgattc ggcctcggcc acggcgcgaa tgcgcgcggc
     7621 ttgagcgcgg ccctccgctt cttgctggat gacgttctgc tccgcgtcgg cgcgggcctg
     7681 ggttatctga ctcgcgccga gctcggcctc gcgctgctca cggaccgccg cttgcttctc
     7741 cgcctctgcg cgagcgatgg cggcaccgtt cgaggcgatt tgcatgttga gcgcgcgctc
     7801 ttgatcgttg attttctgcg cggcctcgag ctgggcgatg cgagcgcgct gttcttcttg
     7861 agcttgcacg acgtcggcgt cggccttggc gcgagcggca gactctcgcc ggcgcgcctc
     7921 ggcgacctcg ctcaaaacgg ccttgatatt caggctgttg aacgagaggc cgaggtccat
     7981 cagctctcgt gagcaggcgt ccttgatgat caccgcgagc tcgtcgtcct cgccgcgcgc
     8041 gactgcgagc gaattcgtcg aggggggagc gagcgcagtc gaagtggccg gcgccgcgga
     8101 gcgcgcgttg aagagctggt cgtgcttgag caggttgatc gcgcgccggc cagccgagga
     8161 gagcacgtcg gtcagggtcg atagctgctc gttgctcgac ttcgaaaaga acttgttcgc
     8221 ggccgtattc accatcgcgt tgttctcgcc cacgctgacg atcgccgagg cgcgcaccgt
     8281 gaccttgacc ggcgcggagc gtccgttggc gtcgagatcc gcagtctgat cggtgatttc
     8341 gatgtcgacg ttgatggctt gggcggggat cacgcgaagc tgggtcacga ccggaacgac
     8401 caaggctttg caggggccgc ggtaaatcct cagatttttg ccaaagctcg cgagcagaat
     8461 ctcgcccgcg tcgacgctac gcaagaacgt cgtgacgatc agcgaagtga ggatgagcac
     8521 gacgacgacg accgcgacgg cgatgatgat agagaagtcc acgtgaatat tcctcggagt
     8581 cgaatgggtt caaggagaac gcggatcgaa gatgaacgcg acggtcacgg tctcgagcgt
     8641 ggcgtcgatg tcctcgacgc gcaccgtatc tcccaccgcc accggcagtc ctgcctggtc
     8701 gtcacgtaac cgagctacga gctgaatcag gcgcccgtcg agctcgacgc tgaccatccc
     8761 gcggccgttg cgaaagggga cgaccgcgcg cgcttcggta tgaatcaacg cttcgagcgg
     8821 cgagctcggc ggcgcctgga agcgaaacaa gagcgagaac agcggcttca cgcacagccg
     8881 ctcgaccagc agcgcgagca gcagcgcgcc caacgccgcg aacgtcggcg agaggtggaa
     8941 agcgcgcacc aatgcgttac cgaacgcgcc gtagagcgcc agtaagctga aaatggcgcg
     9001 cggtgacggc aaccagttga aacgttggcc gggcgccgcg tctaccaaga tttcgccgtg
     9061 ttctcgagct ccagccgctg cgggggcgtg ccgcggcaag gcgcgcgggg cgtgtacgcg
     9121 gccgcccttc agcaaacgca gcgccccacc gcgagcctga ccccaaccat gcgagtgagc
     9181 gtgcgccgca tgaccgtgac cgtgccgccc gaaggcggga atagccatga tcgtcagccc
     9241 aaccagccca gcaaccatga ggagcaagaa caccgccccg ggactctaca tctttttttc
     9301 gtcccggagg cgcggcgacc gcgaacctcg agcctcggct cagttttgtg cagcaagacg
     9361 cgcgaccagg gctcggccgt ccgccgagag cgggtaatca gggaactctt cgagtagctc
     9421 ctcgaagagc gccagcgcat cgctgggacg cttcgcctcc caataggccc aggcggcatc
     9481 gtgaagcggg gagccgtcgc gcggcagcac ctggagagca cgccgcgcat acgagagcgc
     9541 cgcatccgga ttcttggcgt cgcgctcgac gttttgcacg gtgaccagaa ccgcccaatc
     9601 ttccggaaat cgacccaccg cgctccgcgc gagcgccaac gccgcctgcg catctccgag
     9661 acgacgcagc agcacgagct cagccacgta gagaggcgcc tcggctggaa actccgcgcg
     9721 gacccgggcc agcacgtcag cggtggcgcg cacgttggga taccgtgcgt cgccggggtc
     9781 gaggcgcaag ggaagctcgg tccacagctt cacgaaggcg acgagcgggc tcaaaagctc
     9841 tctgagcccc agctgtcgaa tggcgctcgg gctcagccag tcccgaaccc acaccatgca
     9901 ctcgagagaa ggcgcggcga tgatgacgtg tcgaaggagc gcgatggcgt cgagcacttc
     9961 gtgattcatc gccagcacgc gcgttcggca ggcagtcagc gccacgctca ccgagagcgg
    10021 caccaacgtc agcgggtcgc tcgacccacg cgcgatcgag tcgacgaggt cgagcgttgc
    10081 gcgcgagttc ggcaatagca tcagcgcgtc ggaggcacgg agcatggcca attcgagctc
    10141 tccgcgctcg agcgcctttc gcgcttcgtc acggagtggt cgggcttgcg cctcgcgctg
    10201 ctctaaggag ccactcacgg agttcgggtc gatgagattt ttcggggagt ccgacatcgt
    10261 ttcaggatca cttcttggcc gccgagggta cgcctctggt ttcgcctttg tcgcagagac
    10321 gctctccggc gaagttcgtg ccggtggggg taatcgtgac acatcccttg tgacgaatgg
    10381 ttccggccat gagcgagcct cccgccacga cgaactcctt ggttcgcgcg atgcccgaca
    10441 ggtaaatgcc cttcgtccag caatacgaga cggaaccgcc gtacgtagcc acgaactctt
    10501 ggagggctcg cgtgcaggac gcgcacggcg accagtcacc ctccaagtcc atcctgagct
    10561 tcatgctctt cagatcctta ccgcccttgt tcgcccagcc ctctaacaac tcgatcacct
    10621 tccgttcggt gtggttgatg ccagactgga gttggaggct cgtgtcgatg ctctgctcga
    10681 tagtcgaagg aaggctcgag tacgccgcca acattcggtc ctcggccgct ttcacgttgg
    10741 gcgcgaggta cttctcgtac gggcctttgt caccgacctt ctcgagctcg ctacaccaac
    10801 tatcgagggc gctgaaaaag tctccgagcg ccggatcggg gccggggtag ctcttgactc
    10861 cgctctgccc agctcgaggc cgagtaccgc ttttcacgtt gtcggctcgg atcttgtccg
    10921 cttccgcctt cttcgccttc caagctttgc tctcggcgtc cttccgcttc gccccggggt
    10981 tagcctgcaa gtactcgtcg atcacttgtt ccttgtaggt tttcacccaa tcgttccact
    11041 cctgtttgat ctcctcgatt tcggctttcc tctttccgct cggatctttc gggcaccaga
    11101 ccgccttctc tttcaacttc atgttcgcga cgatcttgac gccgatatcc ttgacgaagt
    11161 ccccgttcga cgtcgccgaa cacgaggtct tgtccccgca aatctccgtg aatttcggct
    11221 cgatttcggg caagctaagg acggtgtcgt cggcgatgat gtgaccggtg agcttgtgcc
    11281 cactgtcgcc ggtcgcgctc tcctggttgg tgcaggcgag cttctcttcg ttgcagcgct
    11341 tcgccttcga accgtccgag agcgtgactg tcttcagtgc tttgcagcac tccccgacct
    11401 cgtcacgctc gtcgttcggg ttcgggcaag gaccgttgac gtcgcaggtc tttcgacgct
    11461 tcggatcttt gattttcgcg cattccgccg agctgcacgt gccgtctgga ttttcgacgt
    11521 agcccggttt gcaggaacca tcggggccgc cacgcgtgct ggagcacgag ccgtcggggt
    11581 tcttgctctg gccaggcggg catccgccgt ccgcgccgcc ggttgcgctg cgcccaccgg
    11641 agccgttcga gctgtcacga ccgcccgttc cgctgcgccc gccgttgccg gtatcgttgc
    11701 cgcctttgcc gccgctgccg ttcttcgatg gcccgccgtc gaagcagctc gcgtcggtgg
    11761 aggtcgaagt gttgctggag gtgacgttgc tgagatcggt cggtgcgtac tcgtacttcg
    11821 ctccgtgata ggcgtgcgac gacgtcgcgc tcaggtgcac gaggccgtcg gcgccgcaca
    11881 cgaaacgcaa gcgattgcag cggaaggtcg ccgtgaccgt cgcgtggatc gcgtcgacct
    11941 cttcgggagg attgctcaga ttgacgtaaa attcctcggc gaggtgtggc gacgcagcga
    12001 ggatcatccc cgccgccatg cgcacgctgc cttcgcggat ccccccgagc gaacccttgg
    12061 cctcgcccga gtaaaaggca ggatcgtctg gcaacaccac gaccgcagcg cgcccagccg
    12121 cggaggacgc gcgggcgacg accatttgcg cgacgaggag ctcaccgagc tgccaaaacg
    12181 ccaaaaagca aaacagaacc gggatgtacg
//
LOCUS       DASTKS010001237         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_420764, whole genome shotgun sequence.
ACCESSION   DASTKS010001237 DASTKS010000000
VERSION     DASTKS010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420764"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFQ35_26475"
     CDS             complement(<1..492)
                     /locus_tag="VFQ35_26475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934287.1"
                     /translation="MEKATGLARPSQPPRVSILLPGLYAWAITVALPSSAAHAPWWAR
                     VPAGLACVCLLLAPFAGMLRPRLDLVLGLYGFLGFSLASWAALVSGGFALAVHGLTGA
                     FGAFGWMLFAFAWGELRARRIPENDPHVLSGAPLSPRQAFPRTAEWVLAFGTLGAGLL
                     LLLA"
     gene            complement(516..1997)
                     /locus_tag="VFQ35_26480"
     CDS             complement(516..1997)
                     /locus_tag="VFQ35_26480"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET9934288.1"
                     /translation="MSLSVSLVAHDPLTESADALVVFVFAGALAEHPSLRAIDRALHG
                     ALVAHAKAQEFEGKPEQVLDIPTLGRLKAPRLVVVGAGPEKEITAARLRSVIATGARA
                     AAQSAKTAALVLPSDEEDLRTVGEGIGLGVYRFTKYFTGDRRPKTELGKVLVSQPSSK
                     PSAAAKKSLALGLELAAAVCLARDAINEPPNVLYPETLAKIARQIAQAHQLKIKVLDK
                     KGILAAGMNLHYAVGQGSSNEPRFIHLTYTPRKKKKKLIFVGKGLTFDSGGLCIKPAP
                     GMGDMKSDMGGAAAVLGLMAAVGTLRPDVEVHGIIGAAENMPDGDAYRPADVFTSLDG
                     KTVEIINTDAEGRLVLADALAYAARLKPDVIVDAATLTGAALIALGKSCSAFYTADDG
                     LASAVESAARNAGESFWRMPLLDELAEGLKSDVADLKHTGDRYGGSITAALFLREFVG
                     KVPWIHCDIAGPVLSDRAKGVLPKGGTGHAVLTFTNLVEGLAR"
     gene            2134..3201
                     /locus_tag="VFQ35_26485"
     CDS             2134..3201
                     /locus_tag="VFQ35_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934289.1"
                     /translation="MRMRSGTIASAASLAVLMSWSAPGRAQGAFPLEPEPPAPAPARP
                     APPPTPPPPQPAPPTAPAPAAAPPANTPRPAAPATAQAPAAPPAASPASPPAQALPPS
                     PTAPASPQTAVPPTSTAPTAPQTPGQVAPAPPQPQPATVYEPPPPPPRKPGSDSGRPV
                     LSIRVDPLNWLLDGRLGIELETQIYKFISFEMVPVFVTSELPPNLRWGTDGIVSQESN
                     GVGALAGASIGAGFWLSGKPMRGTVVRAILTNYAYTYKASDENGVFDSVSHVDRHAYA
                     YLGGHSNWGLFTLAGGFGIGVELNKEQRCVSNARVVTSGCRNDELDIALDRTWARAPA
                     NLHSSTYPIELMFRLSLGVTF"
     gene            3216..3926
                     /locus_tag="VFQ35_26490"
     CDS             3216..3926
                     /locus_tag="VFQ35_26490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9934290.1"
                     /translation="MENRSVVVVTGAAGALGSSLVRYLAARGRNVVAVDRPGASQRLE
                     EVAREAPGVESLELESTSETWSSVMERIAKERGPLAGAVLVAGGWAGGKRFFEGDSEA
                     TWRKMMDQNLESTRVALDALLPPMVAAKRGSIVLIGSRAAVRPWESRGAAAYAASKAA
                     LLALVQAIAAEVLEDGVRINAVLPSTIDTPANRKAMPNADPSKWVSRESLCDVIGFLL
                     SDAARDVSGAALPVYGRA"
BASE COUNT          661 a         1358 c         1308 g          646 t
ORIGIN      
        1 ggcgagcaga aggagcagcc cggcgcccaa cgtcccgaaa gcgagcaccc actcggcggt
       61 gcgcggaaac gcttgccgcg gcgagagcgg cgctcccgag agtacgtggg gatcgttctc
      121 ggggatgcga cgggcgcgga gctcacccca agcgaacgcg aagagcatcc aaccgaaggc
      181 gccgaacgca ccggtaagcc cgtgcaccgc gagggcgaac ccaccgctga cgagagcggc
      241 ccagctggcg agcgaaaacc cgagaaagcc gtagagaccg agcaccaggt caaggcgcgg
      301 ccgcagcatt ccggcgaacg gggcgagcag caggcaaaca caggcaaggc ccgccggcac
      361 ccgtgcccac cagggcgcgt gcgccgcgct cgatggcaag gctacggtga tcgcccaggc
      421 gtacagcccg ggcagcagaa tgctgacgcg aggcggctga ctcggacggg cgagaccggt
      481 tgccttctcc atgtcgagcg gcccgcgccg gagcgttagc gagcgaggcc ttcgaccagg
      541 ttcgtgaagg tgagcacggc atgcccggtg cctcccttcg ggagaactcc cttcgcgcga
      601 tcactgagca cgggacccgc gatgtcgcag tggatccacg gaaccttgcc gacgaactcg
      661 cgcaggaaga gcgcggccgt gatcgaaccg ccgtagcgat ctcccgtgtg cttcaggtcg
      721 gcgacgtcac tcttcaggcc ttccgcgagc tcatcgagga gcggcatgcg ccagaagctc
      781 tcgcccgcgt tgcgggctgc gctttcgact gcgctcgcga gcccatcgtc cgccgtgtag
      841 aaggcggagc aagatttccc gagcgcgatg agagccgctc ccgtcagcgt tgccgcgtcg
      901 acgatgacgt ccggcttcaa gcgcgcggca taggcgagcg catccgcgag cacgagccga
      961 ccttccgcgt cggtgttgat gatttcgacc gtcttgccgt cgagcgacgt gaaaacgtcg
     1021 gcggggcgat acgcgtcgcc gtcgggcatg ttctctgctg cgccgatgat gccgtgcact
     1081 tccacgtccg gacgcagcgt tccgaccgcg gccatcagcc cgagcaccgc cgcggcgccg
     1141 cccatgtccg acttcatgtc gcccatgccg ggagccggct tgatgcagag accgcccgaa
     1201 tcgaaagtga gccccttgcc gacgaagatg agcttcttct tctttttgcg gggggtgtag
     1261 gtgaggtgga tgaagcgcgg ctcgttgctg ctgccctggc cgaccgcgta gtgcaggttc
     1321 atcccggccg ccaagatgcc cttcttgtcg agcaccttga tcttcaactg gtgcgcctga
     1381 gcgatctggc gggcgatctt cgcgagcgtc tccggataga gaacgttcgg cggctcgttg
     1441 atggcgtcgc gtgcgaggca caccgcagcg gcgagttcga ggcccagggc gagcgacttc
     1501 ttcgcggccg cgctcggctt cgacgaaggc tgagaaacga gcaccttgcc gagctcggtc
     1561 ttcgggcgac gatcaccggt gaagtacttg gtgaagcggt agacgccgag gccgatgccc
     1621 tccccaaccg tacgcaagtc ttcttcgtcg ctcggtagga cgagtgccgc cgtcttcgcg
     1681 ctttgcgcgg ccgcgcgcgc gcccgtcgcg atcacggagc gcagacgcgc cgccgtgatc
     1741 tccttttccg gaccggcgcc gaccacgacg agccgaggcg ccttcaatcg cccgagcgtc
     1801 ggaatgtcca gaacctgttc gggcttgccc tcgaactcct gcgccttggc atgggccacg
     1861 agcgctccgt gaagggctcg atcgattgcg cgcagcgacg ggtgctcggc gagcgcgccg
     1921 gcgaaaacga aaactacgag cgcgtccgcg ctctctgtga gggggtcgtg agcgacgagc
     1981 gataccgaaa gcgacatttt cgagtagaag tagcactcct ctcgaaaagt tgctccatcg
     2041 agcgcactcc ccccgaacga gcgaggcgga ttggcgaggc gagcccgaag tcgctcgcat
     2101 gcgcgcgcgg ggaacgtcca ctattctctg ggcatgagga tgaggtcggg aaccatcgca
     2161 agcgccgcca gcctcgctgt gctcatgagt tggtccgcgc cgggtcgagc tcagggcgcg
     2221 tttcccttgg agcctgagcc cccggctccc gccccagcgc gacccgcccc gccaccgacc
     2281 cctccaccgc ctcagcccgc cccacccacg gcgcccgctc cagcagctgc gccgcccgcg
     2341 aatacgccgc ggcctgccgc acctgccacg gctcaagctc ccgctgcgcc gcccgcggca
     2401 agccctgcgt ctcccccggc tcaagccctg cccccgagcc caaccgcgcc cgcttcaccg
     2461 caaactgcag tcccgccgac ttcgaccgcg ccgacggctc cccaaacgcc cgggcaagtc
     2521 gctcccgctc ctccgcaacc tcaaccagcc accgtttacg agcctccccc accaccccca
     2581 cgcaaacccg gcagcgacag cggtcgaccg gtactctcca ttcgcgtcga tccgctcaac
     2641 tggctgctcg acgggcgcct cggcatcgag ctcgagaccc aaatctacaa attcatcagc
     2701 ttcgagatgg tgccggtatt cgtcacctcg gagcttcccc cgaacctgcg ctggggcacg
     2761 gacggcatcg tttcgcagga gtcgaatggc gtcggtgcgc tcgccggagc ctctattggc
     2821 gccggttttt ggcttagcgg caagcccatg cgcggcaccg tcgtccgcgc gatcctcacg
     2881 aattacgctt acacgtacaa agcctccgac gagaacggcg tgttcgacag cgtttcgcac
     2941 gtggaccgtc acgcctacgc ctacctcggc ggtcactcga actggggcct cttcacgctg
     3001 gccggcggct tcggcatcgg ggtcgagctg aacaaggaac agcgctgtgt ctcgaacgcc
     3061 cgagtcgtga cgagcggctg ccgaaatgac gaactcgata tcgcgctcga taggacctgg
     3121 gcgagggcgc ccgcgaatct acacagcagc acttacccca ttgagttgat gtttcgtctc
     3181 tcgttgggtg taaccttctg agctgagagg ctctcttgga aaatcggtct gtcgtggtcg
     3241 ttacgggcgc cgcgggggcg ctgggctcgt ctttggtgcg ttatttggcg gcccgtgggc
     3301 gcaacgtcgt cgcggtcgac cgccctggtg cgagtcaacg cttggaggag gtcgctcgcg
     3361 aagctccggg cgtcgagtcg ctcgagctcg aatcgacttc ggagacgtgg tcgtcggtga
     3421 tggagcgcat cgcgaaggaa cgcggaccgc tcgcgggcgc ggtgctcgtc gcggggggct
     3481 gggctggcgg caaacgtttc ttcgagggcg actccgaagc gacctggcgc aagatgatgg
     3541 accaaaattt ggagagcacg cgcgtcgctc tcgatgcgct gcttccgccc atggtggccg
     3601 cgaagcgcgg gagcatcgtg ctcatcggtt cgcgggcggc cgtgcgtccg tgggagagcc
     3661 gaggcgccgc tgcctacgcc gccagcaaag ccgctttgct cgcgttggtt caggcgatcg
     3721 ccgcggaggt cctcgaggac ggcgtccgca tcaacgcggt gctcccgagc accatcgata
     3781 cgccagcgaa tcgcaaggca atgccgaacg ccgacccctc gaagtgggtg agccgcgagt
     3841 ccttgtgcga tgtgatcggg ttcttgttgt cggacgcagc tcgagacgtc tcgggcgcgg
     3901 cgttgcccgt ctacggcagg gcgtgagtca cgggctctcg gtctcccagt aacgctcgac
     3961 ggtgacgtga ccc
//
LOCUS       DASTKS010001238         7321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_868492, whole genome shotgun sequence.
ACCESSION   DASTKS010001238 DASTKS010000000
VERSION     DASTKS010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868492"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..966)
                     /locus_tag="VFQ35_26495"
     CDS             complement(94..966)
                     /locus_tag="VFQ35_26495"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9934291.1"
                     /translation="MQTRKPLRIEVPKDEGGKLARVGLIAVVGFAVGILWPRLAGFRL
                     VPSVPTEKTEASAEAPSADASAAPAAAAPVAAAEPAQTPEAAAPASKENFSVGSGEVA
                     SCRDGKKKQEQCDPIDFDGVARGRIATLAACDAANDISGVLSLGFELDFEKDRVTGVQ
                     AGKSTSLTQKDTDALLGCLKQNLGEVALAGIHHKHERYTVYYKVEFKAPQGDDKKPIP
                     EAAADAAPASGKATVAWDVALVRSGPARDKDVVARVLQGTRVNVVARSGDWYRVKYDA
                     KGNEGWVFRTAIGM"
     gene            1137..2864
                     /locus_tag="VFQ35_26500"
     CDS             1137..2864
                     /locus_tag="VFQ35_26500"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934292.1"
                     /translation="MKACVHCKLNYPNDSGMCFLCGRPLELIQDPRIGTTIAGRYVIE
                     DVIGEGGMATVYSAHHRLVDRPCAIKIMNPNFAKNEVIRERFRREAKAAQKLAHPNII
                     EIFDQGETPEGLVYIVMELLRGVTIADLLDRGQVPVERALPIAIQIARALARAHDLEV
                     IHRDLKPENIFIAEDADGHDVVKILDFGIARSMQDARLTGAGEVFGTPQYMAPERITS
                     IDAGPSADLYAFGVILYEMLAGQLPFDASDVTSFFLKHLKERPVPLRNLAPHVPEGLE
                     RIVMELLEKDPKDRPVDAHRVRADLTAIAELRGVRVPPEFGRDEASSRGPARTLPPVA
                     IDQWVHRIPVFDRMLRTAYPQGEPRLGSLLEEIRHVVTEVGELRSQAVETQRGIESIE
                     SSGRDVRQRFGHAVDALGVDASRARDELKAALAFLNEEKARAEEPREALRTAMREIAV
                     WEGRSGLQVPSRELANAYRSAANIVDAWMATHERVRAAEALLAEKRSVVDDLEFQIQE
                     LRGALAKSEEHVEREQADRQQRVSDLGRRADELEKRLLGLATDFCAPLRRMPALEPLF
                     LELEAGIAA"
     gene            complement(2778..3734)
                     /locus_tag="VFQ35_26505"
     CDS             complement(2778..3734)
                     /locus_tag="VFQ35_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET9934293.1"
                     /translation="MAARSFDPVLKWAGGKRQLLPEILRRLPEKIDTYYEPFVGGGAV
                     FFALAHERRFKKAVLTDQNPDLIAVYRALKSNLKRLMEVLKEHDAKHCEDYYYSVRDE
                     LETTSLVERAARMIYLNKTGFNGLYRVNRSGKFNVPFGRYDKPRILDEPRLIAANKAL
                     ENVEIAIADFEKSCKRAKPGDAVYLDPPYLPVSSTANFSDYHAEPFGIEEHERLASAF
                     GELAERGVYALLSNSHTKDTRAIFSKFEVEVVKARRSINSVKSRRGPVGEILVSNRSV
                     GAKSRGEGKDWVPALSRDAGLELEKKRLERGHAPQRRAKVGR"
     gene            complement(3737..>7321)
                     /locus_tag="VFQ35_26510"
     CDS             complement(3737..>7321)
                     /locus_tag="VFQ35_26510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9934294.1"
                     /translation="SAAPSDARLRDLYSLGITLAELAVPFGREAREPAEVFASAPLSP
                     ALHVLVTALLAKAPGARPSANWVFRQALERLGETEPEQERTARRLATIRGSYLALRRA
                     ELVRASRHDQVEVTVDVPVRHWLVESLSLLREVEALRGGHTHSEKVVIGDLDEVGRAR
                     WLSSVIGDAVAAWPSLPVRGDGELGERLERAVLSHDPETLTFVDLEGAPRAEVAGQSD
                     DPIELALALGRGSVSREVLGRAERWTNLHSTPEALRVALGRALRLRGQLGRALSVLSG
                     STSAEALVETAEVWRRAGDAEQARSLLAHLEPGGLSPTLAARHAAIGARLALDAGQGE
                     RALEMTRDFSASAATGEVRALAQIALGQRREARATLARASLSALDDEQHARVEAVLGN
                     LEHFEGNAEGALSHFQRATEHAARGGAVLEEATYATGVAAAAVHVGELWLARQAARRS
                     LLLFESLGRAREAARAELALASTFAAVGAGVEAREHANSASQRAKTAGDKRCRAYAHL
                     LLADIAAPEDREAVEHARFALTLVDDSDDELRVGARLHERGDAVEIRRFDVRAQGSAA
                     LDARLEWWTARARVASLAQRQDDAQTVLEALLGLVGERGACIVRGPAFAAGAELAAHL
                     GDGDAARRLTRATADAARELSQRAGGELAAEVRSLAWVRLGDSRLTGAFSPEQLADVE
                     NLVRALGRRDRLRPLLEQVVDALVLWTGVERGLLLLRAPGGRLRPRAARNLLRADLDG
                     AQLELSHSLAERALALGEPVVAVDAAGDLPDVHESVHALKLRSVLSVPLIARGEALGV
                     VYLDDRVRRGAFGPRELSWVRLVATLAAVAIADARDQILLRRAARRARRAESRLAAEL
                     AEREAKLDVVERELAHARDARETRFRYDEIVGRSEAMRKTLLLLDRVAASEVPVLLMG
                     ESGSGKELVARAIHRNGARGAESFVAENCGAIPEPLLESTLFGHVRGAFTGADRPRAG
                     LFEIAHKGTLFLDEIGEMSLGMQAKLLRTLQDGEIRPVGGERARTVNVRVIAATHRDL
                     VALVAAGKFREDLYYRLNVISVKIPSLRERVGDVPLLVKHFMKVHGGNRPLTISKAAL
                     DMLSAYSWPGNVRQLENEVRRALVLADDVITPEHLTEAVTSRGEARSIDELNLRQRVD
                     ALEVELVSIALRRTDGNQTRAAELLGVSRFGLQKMMKRLEISLPPGHA"
BASE COUNT         1199 a         2524 c         2434 g         1164 t
ORIGIN      
        1 gggcgggcga gcgagcgagc gggcgggcga gcgggcgggc gagcgagcgg gcgggcgagc
       61 gagcgagcgg gcgagtgagc gggcgagcgg cggtcacata ccgatcgcgg tacggaacac
      121 ccagccttcg ttgcctttgg cgtcgtactt tacacggtac cagtcgccgc tacgcgcgac
      181 gacgttgacg cgtgtgccct gaaggacgcg cgcaacgacg tccttgtcgc gcgcggggcc
      241 gctgcgaact agggcgacgt cccacgcgac cgtcgctttg ccgctcgcgg gtgccgcgtc
      301 cgcggccgcc tcggggatcg gcttcttgtc gtcgccttgg ggcgccttga attctacttt
      361 gtagtagacg gtgtagcgct cgtgcttgtg gtggatgccg gcgagcgcga cctcgccgag
      421 gttttgcttc aagcacccga gcagcgcgtc ggtgtccttt tgagtgagcg aggtgctctt
      481 gccggcttgc acaccggtga cgcgatcttt ttcgaagtcg agctcgaagc cgagcgaaag
      541 gacgcctgag atgtcgttcg cggcgtcaca ggccgcgagc gtcgcgatgc gaccgcgcgc
      601 gacgccgtcg aagtcgatgg gatcgcactg ctcttgcttc ttcttgccgt cgcgacagct
      661 cgcgacctcg ccggaaccga ccgaaaagtt ctctttcgat gcgggagccg cggcctcggg
      721 cgtttgagcc ggctccgcgg cagcgaccgg cgcggccgcc gctggcgccg cgctcgcgtc
      781 ggcagaggga gcctccgcgc tggcttcggt cttctcagtg gggacgctcg gcaccaggcg
      841 gaagcccgca aggcggggcc acaagatgcc gactgcgaaa ccgacgacgg caatcagacc
      901 cacgcgggcg agctttccgc cctcgtcctt ggggacttcg atgcgcagcg gcttcctggt
      961 ctgcacggca ggcccgctct agcatgactt ttggcgcgct tcccagtgac acgctctctt
     1021 gacggccgaa aacgccggcc cgaccccttt ttgtgagcgc gaatctcgcg gcaatttcgc
     1081 tgagcctgat gttcaccctg gaaacctccg ggtgggtcgg caaggattat ccaaggatga
     1141 aagcctgcgt ccactgcaag ctcaactatc cgaacgatag tgggatgtgc ttcctgtgcg
     1201 gccggcccct ggagctgatt caggatccgc gcattgggac cacgatcgcg gggcggtacg
     1261 tcatcgagga cgtgatcggc gaaggcggga tggcgacggt gtattcggcg catcaccggc
     1321 tggtggatcg gccgtgcgcc atcaagatca tgaacccgaa cttcgcgaag aacgaggtga
     1381 ttcgcgaacg ctttcggcgc gaggccaaag ccgcgcagaa gctcgcacac cccaacatca
     1441 tcgagatctt cgatcagggc gagacgcccg agggcctcgt ttacatcgtg atggagctct
     1501 tgcgcggagt cactatcgcc gacctgctcg accgcgggca ggtgccggtg gagcgcgcgc
     1561 tgcccatcgc gattcaaatc gcgcgagcct tggcccgcgc gcacgatctc gaggtgattc
     1621 accgcgacct gaagcccgag aacatcttca tcgccgagga cgccgacggg cacgacgtag
     1681 tgaagatcct cgatttcggc atcgcgcgat cgatgcaaga cgcccgcctg actggcgccg
     1741 gtgaagtgtt cggcacgcct cagtacatgg ccccggagcg catcacgtcc atcgacgcag
     1801 ggccgagcgc cgacttgtac gctttcggcg tgattctcta cgagatgctc gcgggacagc
     1861 tccctttcga cgcgagcgac gtcacgagct tctttttgaa gcacttgaag gagcgaccgg
     1921 tcccgctgcg gaacctcgcg ccgcacgttc cagagggcct cgagcgcatc gtcatggagc
     1981 tgctcgaaaa ggaccccaaa gatcgccccg tcgacgccca ccgcgtgcgc gccgatctca
     2041 ccgcgatcgc ggagttgcgc ggcgttcgag ttcctccgga gttcggccgc gacgaggcct
     2101 cttcgcgggg gcctgcgcgc acgctgccgc ccgtcgcgat cgatcagtgg gtgcaccgca
     2161 tcccggtgtt cgaccgtatg ctgcgcacgg cgtacccgca gggcgagccg cgcctggggt
     2221 cgctgctcga ggagattcgt cacgtcgtga cggaggtcgg cgagctccgg agccaggcgg
     2281 tcgaaactca gcgcggcatc gagtcgatcg agtcgagcgg tcgcgatgtg cgccaacgct
     2341 tcggacacgc cgtcgacgcg ctcggcgtcg atgcaagccg cgcgcgtgac gagctgaagg
     2401 ctgcgctcgc gttcttgaac gaagagaagg cgcgcgccga agagccgcgc gaggctctcc
     2461 gcacggccat gcgcgaaatt gcggtgtggg agggccgctc tggtctgcag gtgccgagcc
     2521 gtgagctcgc gaacgcctac cggagcgccg caaacatcgt cgatgcctgg atggcaaccc
     2581 acgagcgcgt gcgagcggcc gaggcgctgc tcgccgaaaa acgtagcgtc gtggacgacc
     2641 tcgagtttca gattcaagag ctgcgcggcg cgctcgccaa gagcgaagag cacgtcgagc
     2701 gtgaacaagc ggatcggcag cagcgcgtga gcgatctcgg caggcgcgcc gacgagctcg
     2761 agaagcgtct cctcgggcta gcgaccgact tttgcgcgcc gctgcggcgc atgcccgcgc
     2821 tcgagccgct ttttctcgag ctcgaggccg gcatcgcggc ttagagccgg gacccaatcc
     2881 ttcccctcgc cccgcgattt ggcgccgacg ctccggttcg acaccaagat ctcccccacc
     2941 ggacctcgcc gtgacttcac cgaattgatc gagcgccgag ccttgaccac ctcgacctcg
     3001 aacttgctga agatggcgcg cgtgtccttg gtgtgcgagt tcgaaagcag cgcgtagacg
     3061 ccacgctcgg cgagctctcc gaaggcgctc gctaggcgct cgtgctcctc gataccgaac
     3121 ggctcggcgt gatagtccga gaagttcgcg gtcgaagaca ccggcaagta aggagggtcc
     3181 aagtaaacgg cgtcgcccgg cttggctcgc ttacagctct tttcgaaatc ggcgatcgcg
     3241 atctcgacgt tttcgagcgc cttgttggcg gcgatcaaac gaggctcgtc caaaatgcga
     3301 ggcttgtcgt agcgtccgaa cggcacgttg aacttgccgc tccggttcac ccgatagagc
     3361 ccgttgaagc cggtcttgtt cagatagatc atgcgcgcgg cgcgctcgac caggctggtc
     3421 gtctcgagct cgtcgcgcac gctgtagtag tagtcttcgc agtgtttcgc gtcgtgctcc
     3481 ttcaggacct ccatgaggcg cttcaagttg ctcttgagcg cgcggtacac cgcgatgaga
     3541 tccggatttt ggtccgtgag gaccgccttc ttgaagcgac gctcgtgcgc caaagcgaag
     3601 aacaccgccc cgccgcccac gaacggctcg tagtacgtgt cgatcttctc gggcaggcgc
     3661 cgcaaaatct ccggcaagag ctggcgtttg ccgcccgccc atttcagcac cgggtcgaag
     3721 ctgcgcgccg ccatgctcag gcgtgacctg gcggcagcga aatttcgagg cgcttcatca
     3781 tcttctgcag gccgaagcgc gaaacgccga gcagctctgc ggcgcgcgtc tggttaccgt
     3841 ccgtgcggcg gagcgcgatc gagacgagct cgacttcgag cgcgtcgacg cgctgcctca
     3901 ggttgagctc gtcgatgctg cgcgcctcgc cacgtgacgt gacggcctcg gtcaggtgct
     3961 cgggcgtgat gacgtcgtcg gcgaggacga gagcgcgtcg cacttcgttc tcgagctgac
     4021 gcacgttgcc cggccacgaa taggccgaca gcatgtcgag cgccgccttc gagatggtga
     4081 gggggcggtt gcctccgtgg actttcatga agtgcttgac gagcagaggt acatcaccca
     4141 cgcgctcgcg caagctcgga atctttacgc tgatcacgtt caagcggtaa tacaagtctt
     4201 ccctgaactt tccggcggcg acgagagcca cgaggtcccg gtgagttgcc gcgatgacgc
     4261 gcacgttgac ggtccgcgcg cgttctccgc ccaccggccg gatttccccg tcttgcagcg
     4321 ttcgcaggag tttcgcctgc ataccgaggc tcatctcgcc gatctcgtcc aagaagagcg
     4381 tgcccttgtg ggcgatctcg aagagcccgg cgcgcggccg atccgcgccc gtgaacgcgc
     4441 cgcgcacgtg accgaacaaa gtggactcga ggagcggctc agggatcgcg ccgcagttct
     4501 ccgcgacgaa ggactctgca ccccgcgctc cgttgcggtg gatggcgcga gcgacgagct
     4561 ctttaccgct gcccgactct cccatcagca atacgggcac ctcggacgcc gccacgcggt
     4621 cgagcaacag cagcgtcttc cgcatcgctt cgctgcgccc gacgatctcg tcgtaccgga
     4681 agcgagtctc gcgcgcgtcg cgggcgtggg cgagctcacg ttcgacgaca tcgagcttgg
     4741 cctcgcgctc ggcgagctcg gccgcgagtc tgctctcggc acgccgcgca cgccgcgccg
     4801 cacggcgcag taagatttga tcgcgcgcgt ccgcgatcgc cacggctgcg agcgtcgcga
     4861 ccagacgcac ccacgagagc tcgcggggac cgaacgcgcc gcgccgcacg cgatcgtcca
     4921 agtacacgac gccgagcgct tcccctcgcg cgatgagcgg caccgagaga acgctgcgaa
     4981 gcttcaaggc gtgcacgctc tcgtgaacgt cgggaagatc tccggccgcg tccaccgcga
     5041 ccaccggctc ccccagcgcg agcgctcgct cggcgagcga atggctgagc tcgagctgcg
     5101 ccccgtccaa gtcggcgcgg aggaggttcc gcgccgcgcg cggccgcaaa cgcccgcccg
     5161 gcgcgcgcaa cagcaagagc ccgcgttcga ctccggtcca cagcacgagc gcgtcgacga
     5221 cctgctcgag cagcggccgc aagcgatcgc gccgaccgag cgcgcgcact aggttctcga
     5281 cgtcggcgag ctgttctggc gagaacgcac ccgtcaggcg agagtcgccg agccggaccc
     5341 acgcgagcga gcgcacctcc gccgcgagct ctcctcccgc gcgctgcgag agctcacgcg
     5401 cggcgtccgc cgtcgctcga gtgagacgtc gcgcggcgtc tccgtcgccg aggtgcgccg
     5461 cgagctctgc gccggcggcg aaggccgggc cgcgaacgat gcatgcaccg cgctcaccga
     5521 ccaatccgag caaagcctcg agcacggttt gcgcgtcgtc ttgacgttgg gcgagactcg
     5581 cgacgcgggc gcgtgcggtc caccattcga gtcgcgcgtc cagcgccgcg ctgccctgag
     5641 cacgcacgtc gaagcgacgg atctcgaccg cgtcgccgcg ctcgtgcagg cgcgccccga
     5701 cgcgcagctc gtcgtcggaa tcatcgacga gcgtgagcgc gaagcgggcg tgctccaccg
     5761 cctcgcgatc ttccggcgcc gcgatgtcgg cgagcaacaa gtgagcgtac gctcgacaac
     5821 gcttgtcgcc cgccgttttg gctcgctgag acgccgagtt cgcgtgctca cgcgcctcca
     5881 cgcctgcgcc cacggccgcg aacgtactcg cgagcgcgag ctcggcgcgg gccgcttcgc
     5941 gagcgcgccc gagactctcg aacaagagca acgagcgccg cgccgcctgc cgcgcgagcc
     6001 agagctcgcc gacgtgcacc gccgcggccg cgacgccggt cgcgtaggtg gcttcttcga
     6061 gaacggcgcc gccccgtgcg gcgtgctcgg tggcgcgttg gaaatgcgag agggcaccct
     6121 cggcgttgcc ttcgaaatgt tccaggttcc cgagcaccgc ctcgacgcga gcgtgttgct
     6181 cgtcgtcgag cgcggacagg gaggccctcg ccagcgtcgc gcgcgcctcg cgtcgctgtc
     6241 caagcgcgat ctgcgcgagc gctcggacct cgcccgtcgc ggccgaagcc gagaaatcgc
     6301 gcgtcatctc cagcgcacgc tccccctgac ctgcgtcgag agccaagcgc gcaccaatcg
     6361 cggcgtgccg cgccgcgagc gtcggcgaaa gccctcccgg ctcgaggtgc gcgagcagcg
     6421 agcgagcctg ttcggcgtcc cccgcgcgcc gccacacctc ggccgtctcc acgagagctt
     6481 cggccgaagt cgacccgctg agcaccgaga gagcccgccc cagttgcccg cgcagacgca
     6541 acgcgcggcc cagcgcgacc cgcagcgctt ccggtgtcga atgaagattc gtccagcgct
     6601 cggcccgacc gagcacttcg cgcgatacgg agccgcgacc cagcgcgagc gcgagctcga
     6661 tggggtcgtc cgattgaccc gccacttccg cacgcggcgc accctccaaa tcgacgaacg
     6721 tcagagtctc ggggtcgtgc gaaagtacgg cgcgctcgag gcgctccccc agctcgccat
     6781 caccgcgcac agggaggctc ggccaagctg ccacggcgtc gccgatgacg ctcgagagcc
     6841 agcgagctcg acccacctcg tcgagatcgc cgattacgac cttttctgag tgagtgtgac
     6901 cgccgcgtaa agcctccacc tcgcgcaaca acgagagcga ctcgacgagc cagtggcgaa
     6961 cgggcacgtc gaccgtcacc tccacttggt cgtgacgtga agcacgcacg agctccgcac
     7021 ggcgcagcgc caagtagctg ccgcgtatcg tagcgaggcg gcgcgcagtt cgctcttgtt
     7081 cgggctcagt ctcacccaag cgctcgagcg cctgccgaaa tacccaattt gcgctgggcc
     7141 gcgcgccggg cgccttggcc aagagcgccg ttaccaaaac atgcaacgca ggcgagagcg
     7201 gcgcgctcgc gaacacctcc gcgggctcgc gcgcctctct accgaatggc accgcgagtt
     7261 ccgcgagcgt gatgccgagg gaatacagat cgcgcaagcg agcatcgctc ggcgcggcgc
     7321 t
//
LOCUS       DASTKS010001239         4018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1092783, whole genome shotgun sequence.
ACCESSION   DASTKS010001239 DASTKS010000000
VERSION     DASTKS010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092783"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFQ35_26515"
     CDS             <1..589
                     /locus_tag="VFQ35_26515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934295.1"
                     /translation="LAKTEPVIALRERARALPPTWKDTLSKQRTLLANQRARWLDIES
                     DDCANLLALDELLQAARSGDVTNARGEPLGEASVVEWVKAELDVPNWKLVAALAAPNF
                     LSASDGTSERGDDPSNLPDLPDAAEPAAATSATSQHALATLRRLRIASVDRLVREVAR
                     IEPTVTRAAVLAELEAAGKTVSWFGRAILCVRDAS"
     gene            586..2676
                     /locus_tag="VFQ35_26520"
     CDS             586..2676
                     /locus_tag="VFQ35_26520"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET9934296.1"
                     /translation="MSARTQTTETVAVRDGDGESERFSVTEVRNALRCPRVFALGRLT
                     GQSVTFPLGSSCLGATFHRLVERFARQVDVPPSHFASLEAGAARDDVENCLARWLLDL
                     LVDELEQDPTYWSIPGEVDDLAEALREFARHLSARLQGFAGTPSHAVAKLLHAGERSV
                     EARLGQNGPVVHGRLDAIFADQHGALDVIEYKLTDEANEELDRAQVALYREILRVAQG
                     VEAQPVVLRFSPLLREVSMSNDAADRFVASVIEPLLTGMQSWGQQPLNAPATQRRDLC
                     AACPMARPCAEAYPHPLPARDDPPMGAARPRPNVSSAKGASTALPDSDLAGGLRDAAA
                     LTTPGHSKEDETGRIEAEELRDRILAELNRQGVAAVAPKPPVVGPTLYIIEVARPRGA
                     VSHLDRAAEDVRHRLAAESSVDVDYAREGGHRRFVVRRREPRKVMLAPLLELNRAWLS
                     ALPGRLVVGQTPEGSVLCADLADAGTPHLLIAGQSGSGKSVLIQSIIASLVQFHGPAA
                     IRFTLIDPKRVTFIGAGFKAAVAAHLDGPIRFDLEDTLPAIQQLVDAMEERYRIFEAA
                     QVADIREFNEQAPATDHLERRVLVIDEFQDLISDRSGAQAFFSGVKRLGAKARAAGIH
                     LILATQRPDRDTVPPVVKANLGGKIALQVATKINSRIVLDEGGAEKLLGKGDLLANLG
                     RGVVRAQAPLLSER"
BASE COUNT          795 a         1409 c         1166 g          648 t
ORIGIN      
        1 gctcgcgaaa accgaacccg taatcgcact tcgcgaacgc gcacgggcgc tgcctcccac
       61 atggaaggac acgttgtcca aacagcgtac tctcctcgcg aaccaacgcg cgcgttggct
      121 cgacatcgag tccgatgact gcgccaactt acttgcactc gacgagttgc tgcaagcggc
      181 gcgctccgga gacgtaacca acgcacgcgg ggagccgctc ggagaagcca gcgtggtcga
      241 gtgggtaaag gccgagctcg acgtaccgaa ctggaagctc gtcgccgcac tggcagcgcc
      301 gaactttttg agcgcgtccg acggcaccag cgaacggggc gacgatccat cgaacctccc
      361 cgatctcccc gatgccgccg agccagcggc agcaaccagc gcgaccagcc agcacgcact
      421 cgccacgctg cgccggctcc gcattgcctc ggtcgatcgt ctcgttcgcg aggtggcgcg
      481 catcgagccg accgtaacgc gagccgccgt gctcgccgag ctcgaggccg cgggcaaaac
      541 ggtcagctgg ttcggtcggg ccatcttgtg tgtgagggac gcctcgtgag cgccagaact
      601 cagacgaccg agaccgtcgc ggtgcgcgac ggcgacggcg aaagcgagcg cttcagtgtc
      661 accgaggtgc gcaatgccct ccgctgccct cgcgtattcg cactcggaag actgacgggc
      721 caatcagtca ccttccccct cggctcatcg tgcctcggcg ccactttcca tcgcttagtg
      781 gagcgcttcg cgcggcaggt cgacgtaccg ccatcacact ttgcgtcact ggaagcggga
      841 gccgcgcggg acgatgtcga aaactgccta gcgcgttggc tcctcgactt actcgtcgat
      901 gagctcgaac aggatcccac ctattggtcc atccccgggg aagtcgatga tctcgcggag
      961 gctttacgcg agttcgcacg ccacctctcg gccaggctac agggcttcgc cggcacgcca
     1021 agccatgccg tggccaagct cctgcacgcg ggcgagcgca gcgtcgaagc ccgcctcggc
     1081 caaaacggcc ccgtcgtgca cggccgcctc gacgccatct tcgccgatca gcacggtgct
     1141 ctcgacgtca tcgagtacaa gctgaccgac gaggccaacg aagagctcga tcgcgctcag
     1201 gtcgcgctct atcgcgaaat cctgcgcgtc gctcaggggg tcgaagcgca accggtggtg
     1261 ctgcgcttct ctccgctact ccgtgaagtc agcatgtcga acgacgccgc ggatcggttc
     1321 gtcgcgagcg tcatcgaacc gctgctcaca gggatgcaaa gttgggggca gcaacccctg
     1381 aacgcacccg ccacccaacg ccgtgatctc tgcgcagcgt gccccatggc gaggccctgc
     1441 gccgaagcct accctcaccc attgcccgcc cgcgacgacc cgccgatggg cgcggcgcgc
     1501 ccgcgcccca acgtctcaag cgcgaagggc gcgagcaccg ctcttcctga ttcggacctt
     1561 gccggcggtc tacgagacgc cgctgcgctc acgaccccgg gccattcgaa agaagacgaa
     1621 acgggccgaa tagaagccga agagctgagg gaccgaatac tcgccgagct caatcgccaa
     1681 ggcgtcgcag cggtcgcgcc caaacctcct gtcgtgggcc ccaccctcta catcatcgag
     1741 gtcgctcgtc cgcgcggcgc ggtcagccac ctcgatcgcg ccgcagaaga cgtgcggcac
     1801 cgactcgccg ccgagtccag tgtcgacgtc gactacgctc gcgaaggtgg acaccgtcgc
     1861 ttcgtcgttc gacggcgcga accacgaaag gtcatgttgg cgccgctgct cgagctcaat
     1921 cgagcctggc tctccgcgct gcccggtcgc ctcgtcgtcg gtcagacacc agaagggtcc
     1981 gtgctgtgcg ccgatctggc cgacgccggc acgccccacc tcctcattgc gggccaaagc
     2041 ggcagcggaa agtccgtcct cattcagtcc atcatcgcga gcctggtcca attccacggc
     2101 cccgccgcca ttcgcttcac cctcatcgat cccaaacgcg tcaccttcat cggagccgga
     2161 ttcaaggcgg cagtcgccgc ccacctcgat gggccgatcc gcttcgacct cgaagacacg
     2221 cttcccgcaa ttcaacaact cgtcgacgcc atggaagagc gataccgcat cttcgaagcc
     2281 gctcaggtcg ccgacattcg cgaattcaac gaacaagcgc cggcgacgga ccacctcgag
     2341 cgacgcgtcc tcgtcattga tgaattccaa gacctcatca gcgaccgcag cggcgcccag
     2401 gccttcttca gcggcgtcaa gcgcctcggc gccaaagccc gcgcggccgg catccacctc
     2461 attctcgcta cccagcgccc agacagagac accgtcccgc ccgtcgtcaa ggcgaacctc
     2521 ggcggcaaaa tagccctcca ggtcgcgact aaaatcaact cacgcatcgt actcgacgaa
     2581 ggcggcgccg aaaaactcct cggcaaaggc gacctactcg cgaacttggg tcgtggcgtc
     2641 gttcgagccc aagcgcccct cttgagcgag cgctgagcga ggagatcgaa gatcaaagga
     2701 tgcgtcggca actccgcaaa cgtcgacggc cggcttgccc gagctgcaag ccctgagcgc
     2761 gctaccgcga tctctccgga ttcgcttcaa ttgcgcgctt gcagacctcc gctcgcatct
     2821 tctcgtccca cttgaactcc tcgatcgggg cacaaaatga gtacccgtga gcgggtcgcc
     2881 atccaatcac cggataagcc ccctgggaac aacacatacg cagcgtgatc cccccaagag
     2941 cggcgtagga tgtagtgatt gattgtcttc cttccttgct ccagggatag agcgccacgt
     3001 cccacccatt ccgctcgtcc cgccccatcg acaaaggcat gcacgcgaac tgcgtatcct
     3061 cccctttgag cttacgaaaa catagcggag cctgcacgac gattcgctta acatccgccg
     3121 cgtccaaacg ctctttagcg agcggttgta gacactggac gaactcagga gagtgacacc
     3181 ccgagcgcag cgacaggggc ttcactttcg ctcggcaagc cgcccccaag acaaggacgg
     3241 acaatacggc acgaagcaag gccgtcgtcg taaggttggc taacgaggcc acggcgtctc
     3301 ctgtagattg gggttctgat ttgttgcgga gcgggacgcg ttcaacactt ccgtcactca
     3361 cattgccgtg ggttcggaaa gcagacaagc gcgaaacgtg ctcgagccgc ctctgtcgcc
     3421 ccgagctccc ccacgtgtcc cgatagcacg ttccatccca gtcgctgacg tgcaaggcaa
     3481 tcccgcaacc atcgagaccc aatgagcccg tcctcacatt ggacgacgtc ccaatgacga
     3541 tgacgggcgg tccagcacga ttcttgggta gaacgagaac cccggggcgc atcggatcct
     3601 gcgaagttgc aggcggctca gtaccgctac agatcgccgc cccaacgctg tcgaagttga
     3661 gaggcgtcca gccgtaaaac tggggcaagc cgtgaacgtc gcggacaacc gcgccggtgg
     3721 tcgacgatcg atccgtcgcg ctggcgcgca cgagagtgag cgcgccctcg gcggtagatc
     3781 cgtctcgttg accacaatca gcaacaagcg tcagatggta atcaccggcg cgaagtggtg
     3841 gtagcgtcgc cgtgcaaggt acgaatttgc gcttgcaata catcggtgca ccgcgtgtac
     3901 cgcgcgcacc ggagcatccg atcaccatag aaacatagag cgcaattgcc gcgaccttcg
     3961 cgagatcctc cgcgcgaata gggcgcgctg gtcggccatc attgctgatc accacggg
//
LOCUS       DASTKS010001240         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1093117, whole genome shotgun sequence.
ACCESSION   DASTKS010001240 DASTKS010000000
VERSION     DASTKS010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093117"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..663)
                     /locus_tag="VFQ35_26525"
     CDS             complement(376..663)
                     /locus_tag="VFQ35_26525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934297.1"
                     /translation="MRFIQFPDLDLLRDVPERGALLLLLAALDLTDSALRIEHPRLDV
                     NAESLYPPPPPTELLAELLLTRFAELRTLVARYNAAVDDAIGLDPHRDLPF"
     gene            complement(674..1510)
                     /locus_tag="VFQ35_26530"
     CDS             complement(674..1510)
                     /locus_tag="VFQ35_26530"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934298.1"
                     /translation="MNQKLTTSYGLKFHPFRPDVPNEALFVVPSVDAFCRRVEFGLAD
                     GGFVMVTGDPGTGKSVALRLIAQRLRAQRDVVVSTIDHPQSRVSDFYRELGDVFGVPL
                     GAHNRWGGFKALRARWSDHIAQTLVRPVLIVDEAQEMLDSVFNELRLLSSKEFDSRSL
                     LCVVFAGDSRLPDRFRKEQLLPLGSRIRRRLTLDYASRDELLACLDHLLDVAGNPALM
                     TTELKTTLAEHAAGNYRVLMNLGDELLSAALERDLPQLDEKLFFEVFAPPQPKKPAAK
                     KR"
     gene            complement(1507..>2505)
                     /locus_tag="VFQ35_26535"
     CDS             complement(1507..>2505)
                     /locus_tag="VFQ35_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HET9934299.1"
                     /translation="RQKKRKRRAEDAVDQPPRAPREMRSFEVETVHGLWHPDFHQGSR
                     KVLLPSGEYKTPVLLGVLDDRSRLACHLQWYLAETAEALCHGLAQAIQKRGLPRSILM
                     DNGSAMLAAETVEGLMRLGVLQYTTLPYTPEQNAKQENFWAQVEGRLIPMLEGHSPLT
                     LELLNQATQAWVELEYNRGFHSEIGETPIKRLLGGPEVSRKSPSSDELRRAFRVEETR
                     AQRRSDGTITVEGVRFEIPSRYRALRRVNVRIARWDLASVDLVDGRTGQFLTTLLPLD
                     KSKNADGLRRALEPASAEPVAESSGIAPLLQKLMADYAATGLPPAYLPLETSKDQS"
BASE COUNT          439 a          652 c          936 g          478 t
ORIGIN      
        1 tccgtggtct gcccgcactc ccagtctgaa tctgaagcgt ttcatccttt ccttcgaggg
       61 tttcgccaaa gtcgacccag gtagcggact agccttcccc tacaatgatc aaaacggcca
      121 cgccacgatc gggattggtc atcttctgca tcgtggtccg gtcaacggat ccgagccgga
      181 gtgggcaagg cgaggaataa ccttcggtca gggcttagac atactcgagg aggatatcgg
      241 gggttttgtt cgccagcttg ggcaggtggt gagggcgccg gtgacgcagt cacagtatga
      301 cgccctagtt gacgtggcgt tcaacgtgag cgtgaccgcg aagatcgatg tcccgagagt
      361 cgcgttgttt gatggtcaga acgggaggtc tcggtgggga tcgagcccga tggcgtcgtc
      421 cacagcggcg ttgtagcgag cgacgagtgt tcggagttcg gcgaagcgcg tgagcaggag
      481 ctcggccagg agctcggtag gcggtggggg tgggtagagg ctctcggcgt tgacgtcgag
      541 gcgtgggtgc tcgatgcgaa gcgcggagtc ggtgaggtcg agcgcggcga ggagcaggag
      601 gagagcgccg cgttcgggaa cgtcacggag gaggtcgagg tcggggaatt gaatgaagcg
      661 catgggtcgc cgttcaacgc ttctttgcgg cgggcttttt cggctgcgga ggtgcgaaga
      721 cttcgaagaa gagcttttcg tcgagctgag ggaggtcgcg ttcgagggcg gcggagagga
      781 gctcgtcgcc gagattcatg aggacgcggt agttgccggc ggcgtgctcg gcgagagtgg
      841 ttttgagctc ggtggtcatg agcgcgggat tgccggcgac gtcgaggaga tggtcgaggc
      901 aagcgaggag ctcgtcgcga gaggcgtagt ccagcgtgag gcgacggcga atgcggctgc
      961 cgagcggcag aagctgctct tttcggaagc gatcgggcaa tcggctgtcg ccggcgaaga
     1021 cgacgcaaag cagagagcgc gagtcgaact ctttgctcga gaggaggcga agctcgttga
     1081 agacggagtc gagcatctct tgggcttcgt cgacgatgag gacggggcgg acgagggttt
     1141 gcgcgatgtg gtcgctccag cgcgcacgta aggccttgaa tccaccccag cgattgtgcg
     1201 caccgagcgg cacgccgaag acgtcgccga gctcgcgata gaagtcggag acgcggcttt
     1261 ggggatgatc gatggtgctg acgacgacgt cgcgctgggc gcgcaggcgc tgtgcgatga
     1321 ggcggagtgc aacggacttt ccggtgccgg gatcgcctgt gaccatgacg aagccaccgt
     1381 cggcgaggcc gaattcgaca cggcgacaga aggcgtcgac gctggggacg acgaagaggg
     1441 cttcgttggg aacgtctgga cggaacgggt ggaacttcaa tccgtagctg gtggtgagtt
     1501 tctgattcat gattggtcct tgctggtttc gagcggcagg taagcgggag ggaggccggt
     1561 ggccgcgtag tcggccatga gcttttgaag gagtggcgcg atgccgctcg actccgcgac
     1621 gggctcggcg gaagcaggct cgagtgcgcg ccgtaggcca tcggcgttct tgcttttgtc
     1681 gagaggcagg agcgtcgtca ggaactggcc ggtgcgaccg tcgaccagat cgacgctcgc
     1741 gaggtcccag cgagcaatgc gcacgttgac gcgacggagc gcgcgatagc gtgaagggat
     1801 ttcgaagcgc acgccttcga cggtgatggt gccgtcgctg cgtcgctgag cgcgtgtttc
     1861 ctcgacgcga aacgcacggc ggagctcgtc gctgctcgga ctcttgcgcg agacctccgg
     1921 gccgccaagc agtcgtttga tcggtgtttc tccgatttcg gagtgaaagc cgcggttgta
     1981 ttcgagctcg acccacgctt gcgtcgcctg attgagcagc tcgagggtga gcggcgagtg
     2041 gccctcgagc atgggaatga gtcggccttc aacttgcgcc cagaagtttt cctgcttcgc
     2101 gttttgctcg ggcgtgtacg gcagcgtcgt gtactgcaga acgccgaggc gcatgagccc
     2161 ttcgacggtt tcggcggcga gcatggccga accgttatcc atcaagatgg agcgcggcag
     2221 cccgcgcttt tgaattgcct gcgcaagacc gtggcaaagc gcttcggctg tctcggcgag
     2281 ataccattga aggtgacacg cgagtcgcga acggtcgtcg agcacgccga gcaggacggg
     2341 tgttttgtat tcgccgctcg ggagcaggac cttgcgcgag ccttgatgaa agtcgggatg
     2401 ccagaggccg tgcacggttt cgacctcgaa cgagcgcatc tcgcgcggcg cacgcggtgg
     2461 ttgatcgacg gcgtcctcgg cgcggcgctt gcgcttcttc tgtcg
//
LOCUS       DASTKS010001241         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_200997, whole genome shotgun sequence.
ACCESSION   DASTKS010001241 DASTKS010000000
VERSION     DASTKS010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200997"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFQ35_26540"
     CDS             <1..855
                     /locus_tag="VFQ35_26540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934300.1"
                     /translation="ASGGKLSHGGARSSGGESSGGGTVPAGGAHASGGGDECGGAQSC
                     AGTGGQAVGTGGVPADLGKPCEMMSDCSLRTVCDIETGVCAWGCKSSQMPTTEVACAS
                     GQRCVASLKYHPSRCEYSCTESSCPDGLVCQYGRCLRPGTRASGMACDTLTDTRAECA
                     PGLGCASGTCVHLCDPVERNSVCGVGEQCDPLDNCSSDVDPAEIGAPCTTAHSFCAGN
                     GVELLGQCTLERECLLFCDESHSCPDSQTCSSNTCRTPCDATAEEPGCPDGELCRGEF
                     CSSLPGGP"
     gene            complement(863..1504)
                     /gene="lepB"
                     /locus_tag="VFQ35_26545"
     CDS             complement(863..1504)
                     /gene="lepB"
                     /locus_tag="VFQ35_26545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9934301.1"
                     /translation="MRTLWRSLIWIGIVVGVVVGIARAVAIRWWRVPSNDPYLEASIA
                     PTLRGGDLIILWRASPPHLGDLALCPEPKHPDRVVLGRIAANEGETIAIDGATASLNG
                     RRYPVEQTCLEDRFTTLDPQTRIPVEQRCELEKIGSRLHQRGETKETPPEKVEHPDIQ
                     PGQVFLLSDNRQFPYDSRDYGTVDLRTCKETVVFRLVSKAGFGDVANRLTFVH"
     gene            complement(1592..2572)
                     /locus_tag="VFQ35_26550"
     CDS             complement(1592..2572)
                     /locus_tag="VFQ35_26550"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HET9934302.1"
                     /translation="MSVTEPKPLVPESTSVPAPSVHRGSAPPPSEPPRSYVAARARAS
                     SVPPRPSAPIVASALACALAASACAHFGVPASSSAEPEPANAPGEERPSSIPFPDFRI
                     IGEDDFAVRIVTGSVTCSGSLVANDRVLTAHHCVSMRSPRGDIIQKDVDPKAVRVELG
                     GDLLPWGEVEVRAIVAPNCGHAGGDGDLAILVLSRPLRGIPARNVDLDNAPAKGESVV
                     PIGFGRCADAADGIYRKKRVGSPVHKVYEGHVELDAAICPGDSGGPAVSEASGRIVGV
                     VSRSVMDSNEATLGATELARLDAFRPLFAAAEQIAAGVSPSELPPIECRR"
     gene            complement(3152..>4000)
                     /locus_tag="VFQ35_26555"
     CDS             complement(3152..>4000)
                     /locus_tag="VFQ35_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9934303.1"
                     /translation="EVLGLAASLEHSSEHPLAAAATAAARERGAPVSPTTDFEYRPGK
                     GVLGKVRGRSVAAGTALLMDELGIDRSAAPPAEPGASLAFVAVDGRTVGTLSFSDPIK
                     ESAAAALHELREAGLRVVMLTGDGPEAAKAVGDALGLDEVHANVLPGDKAAFVSRLRR
                     EGRRVAMAGDGINDAPALAQADVGIAMGSGTDVAMQSAAITLVQPDLRALGRARRLSS
                     AVMRNIRQNLAFAFVYNLLGVPLAAGILYPAFGLLLSPMIASAAMALSSVSVVTNALR
                     LREAKL"
BASE COUNT          643 a         1265 c         1415 g          677 t
ORIGIN      
        1 gcgtcgggcg gaaagctttc acatgggggc gctcgttcga gcggcggcga gagctcgggt
       61 ggaggaacgg tcccggccgg tggcgctcac gcgagcggcg gcggagacga gtgcggcggt
      121 gctcaatcgt gtgccggcac cggtggtcaa gccgtgggca ccggcggcgt tcctgcggat
      181 ctcggcaagc cctgcgagat gatgagcgac tgcagtctca gaacggtgtg cgacatcgag
      241 actggtgtgt gcgcctgggg ttgcaagtcg agccagatgc cgacgaccga ggtcgcatgt
      301 gcgagcggac agcggtgtgt cgcgagcttg aaataccatc cgtcgcgctg cgagtactcg
      361 tgcacggagt cgagctgtcc agatggtctc gtttgccaat acggtcgttg tttgcgtccc
      421 ggcactcgcg cgagcggcat ggcgtgcgac acgctgaccg atacacgcgc cgagtgtgca
      481 ccgggcctgg gctgcgcgag cggcacgtgc gtgcacctgt gcgatcccgt ggaacggaac
      541 tcggtgtgcg gcgttggaga gcaatgcgac ccgctcgaca actgcagttc ggacgtcgat
      601 ccggcggaga ttggcgctcc gtgcacgacc gcgcattcgt tctgtgccgg caacggtgtc
      661 gagctgctcg gtcagtgcac gctcgagcgc gagtgcttgc tcttctgtga cgagtctcac
      721 agttgtccgg actcgcaaac gtgcagttcg aacacgtgtc gaacgccgtg cgatgcaaca
      781 gccgaggagc cgggctgccc ggacggcgag ctgtgccgcg gagagttttg tagctcgctg
      841 cccggcggcc cttagtggcc ccttagtgga cgaacgtcag tcgattcgcg acgtcgccga
      901 agcccgcttt gctgacgagg cgaaagacca ccgtctcttt gcaggtgcgg aggtcgacgg
      961 tgccgtagtc gcgcgagtcg taggggaatt gtcggttgtc gctgagcaag aagacttggc
     1021 ccggctgaat gtcggggtgc tcgacctttt cgggcggcgt ctctttcgtt tcgccgcgct
     1081 ggtgtaggcg tgagccgatc ttctcgagct cgcaacgctg ttcgacgggg atgcgagtct
     1141 gcgggtcgag cgtcgtgaag cggtcctcca agcaagtttg ctcgaccgga tagcggcgac
     1201 cgttgaggga ggcagtcgcg ccgtcgatgg cgatggtctc gccttcgttc gcagcgatgc
     1261 ggccgagaac cacgcgatcg ggatgcttgg gctccgggca caacgcgagg tcgcccagat
     1321 gcgggggact cgcgcgccac aagatgatga ggtcgccgcc gcgcagcgtc ggggcgatcg
     1381 aagcttcgag gtaagggtcg ttcgagggca cacgccacca acggatcgcc acggcgcgcg
     1441 caatgccgac cacgacgccg accacgatgc cgatccaaat cagcgaacgc cacagagtgc
     1501 gcatcgatct cggagctgag actagccgat ttacttcgtg gcggattcgc gctctggtga
     1561 agtcagtagc gtgatgtagg gaggtgcgcg ctcagcggcg acactcgatc ggcggtagtt
     1621 ccgacgggct cactcccgcg gcaatctgct ccgcggcggc gaacagcgga cgaaaagcgt
     1681 cgagccgcgc gagctcggtc gcgccgagtg tggcttcgtt gctatccatc acgctgcgcg
     1741 agacgacgcc gacgattcgg ccgctcgctt cgctcaccgc gggtcccccg gagtcgcccg
     1801 ggcaaatggc ggcatcgagc tcgacgtggc cctcgtacac tttgtgcacc ggcgagccga
     1861 cgcgcttttt gcggtagatg ccgtccgccg cgtcggcgca acgaccgaag ccgatcggca
     1921 cgaccgactc ccctttcgcg ggcgcattgt cgaggtcgac gttgcgggcc ggtatgccgc
     1981 gcaagggccg cgaaagaacc aggatcgcga gatcaccgtc gccgcccgcg tgtccgcagt
     2041 tcggcgcgac gatggcgcgc acctcgactt ctccccaggg cagcaaatcg ccgcccagct
     2101 cgacgcgcac ggctttgggg tcgacgtcct tctgaatgat gtcgcccctc ggcgagcgca
     2161 tcgacacgca gtgatgggcg gtgagcacgc ggtcgttcgc gacgagcgaa ccggagcaag
     2221 tcaccgagcc ggtgacgatg cgcaccgcaa agtcatcctc gccgatgatg cgaaaatccg
     2281 gaaaaggaat gctcgagggc cgctcttcgc ccggcgcgtt cgcgggctca ggctctgcgg
     2341 acgagctcgc cggcaccccg aaatgcgcgc aagcgctcgc ggcgagggca catgcgagcg
     2401 cgctcgcaac gatcggtgcc gacgggcgcg gcggtacgga cgacgcacgt gcgcgcgcgg
     2461 ccacgtaaga gcgcggcggt tcggaaggcg gcggagccga gccgcgatgg acgctgggcg
     2521 ctggcacgct ggtgctttcg gggaccaagg gcttgggttc cgttactgac accgccgctc
     2581 gctcggagtt ccgactccga tgattgtgcc accttgggtc cctccgtaac gattttattt
     2641 ttgggtgcca tcgctgagat cactgcgagt cgttccgtgc ccgatcagcg cctctgtgcg
     2701 gcctacatcg ctccacacct gacgcgcagt gtcgaaccgc gagcgccacg caacccctcc
     2761 ctctcttctc actcatccgc tcttggaacg ccggcccgtc cctggtcgcc gagagccccc
     2821 tcgccgagag cccgctcgcc gagagctccg tcggggccgc gcggggccct tccccgctgg
     2881 ttggccttcg cgatgctgcc atgaccagag cggttggctc gaggctcgcg aggttttggg
     2941 gcgggggcct tcccacgggc actgagcgtt tggcgacctt agaaactcgg gcgatttgcg
     3001 gattggaact cggtgtttgg gatttcaagc atcggaacgg actctttcgg aggacgagcc
     3061 gaaagagtcc gttcatgccg agctctcgcg aagcgaaagg gatgaaggct ctctgcgagc
     3121 gagacgcgac gaacgagccg ctggccgggc ctcagagctt cgcttcgcgc aggcggagcg
     3181 cgttcgtgac gaccgaaacc gagctcagtg ccattgccgc gctcgcgatc atcgggctca
     3241 agagcagccc aaaagccgga tacaggatgc ccgccgcgag cgggacgccg agcaggttgt
     3301 aaacgaacgc gaaggcgagg ttttgccgga tattgcgcat cacggcggag gagaggcgcc
     3361 gagcgcggcc caaagcgcgc aaatcaggct ggaccagcgt gatggcggcg ctctgcatgg
     3421 ctacgtcggt gccgctgccc atggcgatgc cgacgtcggc ctgagccagt gccggcgcgt
     3481 cgttgatgcc gtcgccggcc atggcgacgc gccgtccttc gcggcgtagg cgagagacga
     3541 acgcggcctt gtcgccgggc agtacgttgg cgtgcacctc gtcgaggccg agcgcgtcgc
     3601 ccacggcttt tgcggcttcg ggcccgtcac ccgtgagcat gacgacgcgg aggccagcct
     3661 cgcgcagctc atgcagagca gcggcggcgg attccttgat tggatcactg aagctgaggg
     3721 tgcctactgt acgtccgtcc accgcaacga acgccaagct cgctccgggt tcggcaggcg
     3781 gcgccgctga gcgatcgatg ccgagctcgt ccatcaagag agcggtgccc gcggcgaccg
     3841 agcgcccgcg aacttttccg agcacgccct tgccggggcg gtactcgaag tcggtggtgg
     3901 gcgagacggg cgcgccgcgc tcccgggcgg ccgcagttgc cgcggcggcg agcgggtgtt
     3961 cgctgctgtg ctcgaggctc gcggcgagtc cgagcacttc
//
LOCUS       DASTKS010001242         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1764245, whole genome shotgun sequence.
ACCESSION   DASTKS010001242 DASTKS010000000
VERSION     DASTKS010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764245"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VFQ35_26560"
     CDS             <1..1477
                     /locus_tag="VFQ35_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9934304.1"
                     /translation="THFKFPEDHGSFARATLRCVGVGKCRRLNGTPHDDTMCPSFMVT
                     REERHSTRGRAHLLWEMLRREGVPVEGGWRDESVKEALDLCLSCKGCKGDCPVNVDMA
                     TYKAEFLSHYYDGRVRPRHAYAFGLIDRWARWASVAPNLVNLVTQTPGLARLAKGLAG
                     MAPERSIPAFAPETFRAWFQRRGAKPARGTKVVLWPDTFNNHFYPDTARAAVEVLEHL
                     GFDVEIPSRPLCCGRPLYDYGMLDRAKAYLEEVLTVLGPYVSAGTPLVVLEPSCCAVF
                     RDEMPALLPNRNEAKSLSRQTFLLSEFLAQSPRPRLPILRRAAVVQGHCHHKSVLEFE
                     HERESLERMQLDARVLSSGCCGMAGSFGFEADKYDVSLACGERSLLPEVRGQSRQTLV
                     IANGFSCRTQIAQTTEREALHLAEVMKLALDYGPSGPPIEGAPETPVVERRKRELRRG
                     RKLLALRAALALGAIAGATLWWHRRHAVAPRSKGLFHAFTG"
     gene            1575..>2474
                     /locus_tag="VFQ35_26565"
     CDS             1575..>2474
                     /locus_tag="VFQ35_26565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934305.1"
                     /translation="MKLRRWAPLWSFAAGCLSGAMLLGWHLGHTPSLAPRESRAPERD
                     VEPLRASETKAAPTVAYTPSVERSEREPAAHLAVDENVEAPSEHGSSVADVLARLEAE
                     YRERSVSKQTTSSAVEAKPSVSAADPVEATPAVAASGSPGELPHVDSAAKASPEPIRV
                     ATVSEPVAAKRTVPDEVPPPVVPPPHASTSTPAGASAEVTLVAATAPVTLDEASPSTP
                     TGPSVPPLVAPSLPQAASPQQIALVQQTALMQQAALAQQVVMLQYLQLVSLGTNARVG
                     APAVQAGRRPPGGRIVDTLPSTFS"
BASE COUNT          415 a          802 c          824 g          433 t
ORIGIN      
        1 cacccacttc aagtttccgg aggaccacgg cagctttgcg cgagcgactc tccgctgcgt
       61 tggcgttggt aagtgccgca ggttgaacgg cactccccac gacgacacga tgtgtccgag
      121 cttcatggtc acgcgcgaag agaggcactc gacgcgtggt cgcgctcact tgctctggga
      181 aatgctgcgt cgggaaggcg ttccggtcga gggcggatgg cgcgacgaaa gcgtcaaaga
      241 ggctttggat ttgtgcctgt cgtgcaaggg atgcaaaggg gactgtcccg tcaacgtcga
      301 catggcgact tacaaggccg agtttctgtc gcactactac gatggcagag tgcgaccgcg
      361 acacgcctac gcgttcgggc tcatcgatcg ctgggcgcgc tgggccagcg tcgcaccgaa
      421 cctcgtgaac ctcgtaaccc aaactcccgg gctcgcgcgg ctcgcgaagg gcctcgcggg
      481 catggctccc gagcgctcga tccccgcgtt tgctccagag acgtttcggg cctggttcca
      541 gcgccgaggc gcgaaaccgg cgcggggtac caaagttgtt ctgtggcccg acacgttcaa
      601 caaccatttt tatccggaca ccgcgcgtgc cgcggtagag gtcctggagc atctcggctt
      661 cgacgtggag attccgagcc gcccgttgtg ttgtggtcgc ccgctctacg actacggcat
      721 gctcgatcgc gccaaggcat acctcgaaga agtgctcacg gtgttgggtc cgtacgtcag
      781 cgcgggcact ccgctcgtgg tgctcgagcc gagctgttgc gcggtgtttc gcgacgagat
      841 gccggcgctc ctgccgaacc gaaatgaagc gaagagtctt tcgcgccaga ccttcctact
      901 gagcgagttt ttggcgcaaa gtccgagacc cagactgccg attctacggc gcgcggcggt
      961 ggtgcaaggg cattgccatc acaagtcggt gctcgagttc gagcacgagc gggagtcgct
     1021 cgagcgcatg cagctcgacg cgcgcgtgtt gagctctggt tgctgcggca tggccggctc
     1081 cttcgggttc gaggctgaca agtacgacgt ttcgctcgct tgcggggagc gctcgttgtt
     1141 gcccgaggtg cgaggtcaga gcaggcaaac gctggtcatt gcaaacggct ttagctgccg
     1201 cacgcagatt gcgcagacca ccgaacgcga agcgctgcac ctcgctgagg tcatgaagct
     1261 agcactcgac tacggcccat cgggaccccc catcgaggga gccccggaga caccagtcgt
     1321 cgaacggcgg aaacgagagc ttcgacgagg acggaagttg cttgcgttga gggcggcgct
     1381 tgcgctcgga gccatcgccg gcgcgacgct gtggtggcac cgaaggcacg ctgtcgcgcc
     1441 tcgctcgaaa gggctgttcc acgcgtttac cggatagctc cgcaaatttt cagtgtcccc
     1501 ccgggtgctt ccgcgacgcg ggtagcgcac cttcgcgccc cgccgccgcg cgctggcgtg
     1561 ctaaggtgtc gccgatgaag ctgcggcgct gggcaccact ctggagcttc gcggctgggt
     1621 gtctcagcgg ggcgatgctg ctcggctggc acctcggaca cactccgagt ctcgctccgc
     1681 gcgaatcgcg tgcccccgag cgcgacgtgg agccgctgcg tgcgtccgag acgaaggcgg
     1741 ccccgacagt tgcctatacg ccgtctgtcg agcgaagcga acgagagccc gcggcgcatt
     1801 tggcagtcga cgaaaacgtg gaggcgccgt ccgagcatgg cagttcggta gcggacgtat
     1861 tggcgcgctt ggaggccgag tatcgcgagc gcagcgtgtc gaagcagaca acctcgagcg
     1921 ccgtcgaagc aaagccttcg gtgagcgcgg ccgacccggt cgaagcaact cctgctgtcg
     1981 ccgcttcagg gagccctgga gagctgcccc acgtcgattc ggccgccaaa gccagtccgg
     2041 agcccattcg tgtggccacc gtctccgagc cggttgcggc caagcgaacg gtgcctgacg
     2101 aagtgcctcc gccagtcgtg ccgccaccgc atgcctcgac ttcgacgcca gctggcgcgt
     2161 ccgccgaggt cacgctcgta gcggcgaccg cccccgtcac tctggatgaa gcgtccccgt
     2221 cgacaccgac gggaccgtcg gtaccgccgc tcgtcgcccc gtcattgccg caagccgcga
     2281 gtcctcagca gatcgccctg gttcaacaaa cggcgctcat gcagcaggcg gcgctcgctc
     2341 agcaagtggt gatgctgcag tacctgcagc tcgtgtcgct cgggacgaac gcacgggtcg
     2401 gcgcgcctgc tgttcaagcg ggacggcgac ctcccggggg gcgtatcgtc gatactctgc
     2461 cgagcacgtt cagc
//
LOCUS       DASTKS010001243         5482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_422563, whole genome shotgun sequence.
ACCESSION   DASTKS010001243 DASTKS010000000
VERSION     DASTKS010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422563"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1009)
                     /locus_tag="VFQ35_26570"
     CDS             complement(47..1009)
                     /locus_tag="VFQ35_26570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9934306.1"
                     /translation="MKRPWLRALLPLALVLVLGLVFNGNGAFFEWQTHRALLREISVH
                     GVLACGLTLVIVAGGIDLSVGSVLALSAVSFSLLTMHAGLTSGLALVIVLAVGLVAGL
                     VSGALVAFARIQAFIVTLAMMVFARGLAKQLSGGQKVSHYFAAPDGHFVNVEPPRLFA
                     LVDSRVLADNLSVVTLVFAACAVACYVVLARLRVGRFLYAIGGNWDAARLSGVPVRLA
                     ALFAYAASGFLSALAGVCQAAQETQGDPETGLGYELDAIAMVVLGGTSLAGGRGGIGL
                     TLIGALTLGYLQKVLSLNAYSTEVRLMLTGVILIGAVLFQRRRA"
     gene            complement(1006..2463)
                     /locus_tag="VFQ35_26575"
     CDS             complement(1006..2463)
                     /locus_tag="VFQ35_26575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9934307.1"
                     /translation="MSFGATRALSDVSLTFKASEVHVIAGGNGAGKSTLIKILSGVHN
                     DYEGELLLDGRPLRLADPGAARRAGIATIFQELSLVGSLSIADNLSLSCPGSSFAPRS
                     SRAARAYAMAALGRVGLDRDPDLLVEELPLSERQLVEVARAIAGDASVLIMDEPTSAL
                     SEPEVERLFKTLRELLKRNVSVIFISHRRDEVERIAEHISVLRDGKVVLSAPAREVTH
                     DALIHAMIGRALSPLAESVLPKEAGAVSLEVDGLSTSGLGACRAVSFQALQGQITGLA
                     GLAGSGASALLSALAGEHRATAGRMRLAGAPYAPRGARDAWSRGVAFLPTDRAHSVFP
                     ELSLHWNATLSALRRFERYEWMDWELELGAARAAAKRARLAASDWSLPAGALSGGNQQ
                     KLALTRCLLAEPRVLLLDDPTRGVDAAAKADVARLLRELAASGVCVLLSSSELDELVS
                     LCDRVLIFFKGEVARVLEGAELCREDVLRCMSGGV"
     gene            complement(2542..3774)
                     /locus_tag="VFQ35_26580"
     CDS             complement(2542..3774)
                     /locus_tag="VFQ35_26580"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9934308.1"
                     /translation="MDNATHAIVGLLVAEVVIQYRGRRGRVSTAGFLASSRWVSALAN
                     NLADLDSLYAKRLGGKLGYLLQHRGYTHTVPAALAIGFTLWAVWLGMRRRALTRADRW
                     WLLVLSLGGALLHIAFDACNNYGVHPFWPLFDGWLYGDCLFIIEPWLWVFAVPALYPA
                     LTNRWAKRALGAVLAAGVGLAWLLRIAPWGVALALTLGTLAALRACYRLSSRHRLYFA
                     LAGWGAVFVAFAGASRLGRASVARELSARPDEHLEEIVMTPAPGNPLCFSVVTVSTRR
                     GDYLLRAARASALPTVLDAEACRVQATGLTLGLRAPSIRSNETVYWEGDWQRPLDELV
                     GLDASHCLVHALLVFARAPFWKPLPNQRLLLGDLRFDRDPDYDFDELEVPGHPEAGQC
                     LRWIPPWRPPRESLLRGR"
     gene            3923..5440
                     /locus_tag="VFQ35_26585"
     CDS             3923..5440
                     /locus_tag="VFQ35_26585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9934309.1"
                     /translation="MLDCVPCATRAPTEHVYYSMTKGLCSTCKRSVDVKIHFRGEQVF
                     FDKFCPEHGAQSCLVSSSVEWYLDALSFIAPNTPPRGETKPVQHGCPFDCGPCKSHQQ
                     KVYMPVIPITSACNLDCPICYTVNKNDDAHMLSEADFQRILDHLLVQHDELDIINFTG
                     GEPTMHAKLPQFLRMARAAGIRRLTISTNGLKLLNESFVEELAALDARIVLSLDTFDE
                     DIDMKMLGAKTVKAKLKVLDLLEKHDVTTTLLPAVAAGLNDRDVGALFELMLSKPNIC
                     SLELHTLTFTGQGGVGFSRGARITTPDLHRILAEATGGRLRWTDFVPSPLAHPHCYSI
                     CYVLMLDDGGYVPFTRFMSRAELFELLSDSIYIQPRANTERVLRNSIDELWANPDSVP
                     EAERVLATLKRLVGELFPSRGPISLEARQKIAERASKAIYIHSHMDEESFDVSRIMKC
                     SVGVPEVDGSNIPTCSYNVLYRERDPRFADASMLKRMSDFRPPAAVAARSLPIVK"
BASE COUNT         1094 a         1854 c         1687 g          847 t
ORIGIN      
        1 agagctccgt gtcgtcgcga cctcggcgtt cgcggcgacg cctgcgctac gcgcgccgcc
       61 gttgaaagag caccgcccca atcagaatga cgccggtcaa catcagccga acctccgtgc
      121 tgtaggcgtt caagctcaag accttctgta agtagccgag ggtcagagct ccgatcagag
      181 tcaggccgat tccgccgcgc cctccagcca agctcgtccc cccgagcacg accatcgcga
      241 tcgcatcgag ttcgtagccg agccccgtct ccggatcgcc ctgcgtctct tgggccgcct
      301 gacagacccc tgccagggcc gataagaagc ccgacgctgc gtacgcaaag agcgccgcca
      361 gccgcacggg cactcccgaa aggcgcgccg catcccagtt gccaccgatc gcgtagagaa
      421 agcgtccgac tcgcagccgc gcgagcacca cgtagcaagc caccgcgcac gccgcgaaca
      481 ccagcgtgac caccgaaagg ttgtcggcga gcacgcggct atccaccagg gcaaacagtc
      541 gcggcggctc gacgttcacg aaatggccgt ccggcgccgc gaagtagtgc gacacctttt
      601 gccctcccga gagctgcttg gcgagaccac gcgcgaacac catcatggcc agagtcacga
      661 taaaggcttg aatccgcgcg aacgcgacca gcgctcccga gaccagcccc gcgactaggc
      721 ccactgcgag gacgatgacc aacgcaagcc ccgaggtcaa tccggcgtgc atcgtcaaca
      781 gagaaaagct caccgccgag agcgcgagca cgctgcctac cgaaagatcg atgccccccg
      841 cgacgatcac gagcgtgagg ccacacgcga gcacgccgtg aaccgatatt tcccggagca
      901 aggctcggtg tgtctgccat tcgaaaaacg cgccgtttcc gttgaacacg agcccgagca
      961 ccagcacgag tgcgagtggc aacagcgcgc gcaaccaagg ccgtttcaca cgccccccga
     1021 catgcagcgc aagacgtctt cgcgacacag ctcggcgcct tccaagacgc gagccacctc
     1081 acccttgaag aatatcaaaa cccggtcgca caacgacacg agttcgtcga gctccgaact
     1141 cgagagcagc acgcacaccc cgcttgccgc gagttctcgc aggagccgtg ccacatcggc
     1201 cttggcggct gcgtccacac cgcgcgtggg gtcgtcgagc aagagcactc gtggctcggc
     1261 gagtagacat cgagtgagcg cgagcttttg ctggttacca ccagagagtg cgcccgccgg
     1321 cagagaccaa tccgacgccg cgagccgagc tcgttttgca gcggcacgcg ccgcaccaag
     1381 ctcgagctcc caatccatcc attcgtagcg ctcgaaccgc cgaagcgcag aaagcgtcgc
     1441 gttccagtgc aagctgagct ccggaaagac gctgtgcgcg cgatccgtag gcaagaacgc
     1501 gacccccctc gaccacgcat cgcgtgcgcc gcgcggagcg tacggagcgc cagcgagccg
     1561 cattcttccc gccgtcgcgc gatgctctcc agccaaggcc gacaagagcg ccgaagctcc
     1621 ggagcccgcg aggccagcga gccccgtgat ttggccctgc aaagcttgga agctcacggc
     1681 gcggcacgcc ccgagcccag acgtggaaag gccatcgacc tcgagggaga cagcgcccgc
     1741 ttccttcggg agaacgcttt cggcgagcgg tgacaatgct cttccaatca tcgcgtgaat
     1801 gagggcatcg tgtgtaactt cgcgcgcggg tgcgctcagg acgaccttgc cgtcgcgcag
     1861 cacgctaatg tgctcggcga tgcgctcgac ttcgtcacgt cggtgcgaga tgaagatcac
     1921 gctgacgttc cgcttcagga gctcgcgtag cgtcttgaag agccgctcga cctccggctc
     1981 cgagagggcg ctcgttggct cgtccatgat gaggacggag gcgtcgcccg caatcgcgcg
     2041 cgcgacttcg actagctgac gttcgctcaa cggaagctcc tcgacgagaa ggtccgggtc
     2101 tcggtcgagc ccgacgcgcc cgagcgccgc catggcgtag gcccgcgcgg cacgactcga
     2161 cctcggcgcg aacgacgaac cagggcaaga caagctcaag ttgtctgcga tcgagaggct
     2221 ccccacgagc gagagctcct ggaagatggt agcgatgcca gcgcgtcgcg cggcgcccgg
     2281 atccgcgagg cggaggggcc gaccatcgag cagcagctct ccttcgtaat cgttgtgtac
     2341 gccggaaaga atcttgatga gcgtgctctt cccggcgcca tttccgccgg cgatgacgtg
     2401 cacctcagag gctttgaagg tgagcgacac gtcgctgaga gcgcgcgtcg caccgaagct
     2461 catcgagacc cgacggagct cgagccgaat gggtggagat ggcgcgacct cggctggaat
     2521 gggtcgaggc tagcaagctc ctcagcggcc gcgaagcagg ctttcgcggg gagggcgcca
     2581 gggcggaatc cagcgcaaac actggcccgc ctcgggatgg ccgggcacct cgagctcgtc
     2641 gaagtcgtag tccggatcgc gatcgaagcg caaatcgccc aggagcaagc gttgattcgg
     2701 cagaggtttc cagaacggag cccgtgcaaa gaccagcaag gcgtggacca ggcagtgaga
     2761 tgcgtcgaga ccgacgagtt cgtcgagcgg tcgctgccag tcgccctccc agtaaacggt
     2821 ctcgttcgac cgaatgctcg gggctcgcag cccgagagtg aggcctgtcg cctggacgcg
     2881 acaagcttcg gcgtcgagca cggtcggcag cgcagaggcc ctcgcggcgc gaagcaagta
     2941 gtcgccccgg cgcgtcgaca ccgtgaccac actgaagcaa agcgggttgc cgggcgccgg
     3001 cgtcatcacg atctcttcga ggtgctcatc cggccgcgcc gagagctcgc gcgcgacgct
     3061 ggctcgaccg agccgcgatg cgcccgcaaa cgccacgaac accgcgcccc agcccgcgag
     3121 cgcgaagtag agccgatgcc gcgacgaaag gcgatagcac gcacgcagag ccgcgagcgt
     3181 tccgagcgtc aatgcgagcg cgactcccca gggagcgatc cgcagcagcc aggcgagccc
     3241 gacgcctgcg gccaagacag ccccgagcgc gcgctttgcc catcgattcg tgagcgccgg
     3301 gtacagcgcc ggaacggcga acacccacag ccacggctcg atgatgaaca aacaatcgcc
     3361 gtagagccag ccgtcgaaca acggccaaaa cgggtgcacg ccgtagttgt tgcaagcgtc
     3421 gaaagcaatg tggagcagcg cgcccccgag ggagagaacg aggagccacc atcggtctgc
     3481 cctcgtcaga gctcttcgcc gcatcccgag ccaaaccgcc cacaacgtga agccgatcgc
     3541 gagcgcagca ggcaccgtgt gcgtgtaccc tcgatgctgc agcaaatatc cgagctttcc
     3601 gccgagacgc ttcgcgtaca gagagtcgag gtcggcgaga ttgttcgcga gcgcgctgac
     3661 ccaacgcgag cttgctagaa agccagccgt cgaaacgcgc cccctgcgac cccggtactg
     3721 aatgaccact tccgcgacga gtagtccgac gatcgcgtgg gttgcgttgt ccacggggcg
     3781 aagcctgacc gctctttttg tcccgagctc agaaatcgac cgcgctattt cggtcggcgg
     3841 ggctgcatgc taggttcctt ggccgaacgc ggggtcgctc acgagcttcc cgctggccgt
     3901 ttggtggaag ctggaggatc cgatgctcga ttgcgtgcct tgtgcgacac gagctccgac
     3961 ggagcacgtg tactactcga tgaccaaggg gctatgcagc acgtgcaagc gaagcgtgga
     4021 cgtcaaaatc cacttccgcg gcgaacaggt cttcttcgac aagttctgcc ccgagcacgg
     4081 agcacagtcg tgcctcgtct cctcctcggt cgaatggtac ctcgacgcac tctccttcat
     4141 tgcgccgaac acgccgccgc gcggcgaaac caagccggtg caacacggat gccccttcga
     4201 ctgcgggccc tgtaagtcac accagcaaaa ggtctacatg ccggtgatcc ccatcacttc
     4261 ggcgtgcaac ctcgattgcc cgatttgtta cacggtgaac aagaacgacg acgcgcacat
     4321 gctttcagaa gcagacttcc agcgcatcct cgatcatctg ctcgttcagc acgacgagct
     4381 cgacatcatc aatttcaccg gtggcgagcc gaccatgcac gcgaagctgc cgcagttttt
     4441 gcgaatggcc cgcgccgccg gcatccgcag gctcaccatc tcgacgaacg gactcaagct
     4501 cttgaacgag agcttcgtcg aagagctcgc cgcactcgac gcgcgcatcg tgctctcgct
     4561 cgacacgttc gacgaagaca tcgacatgaa gatgctcggg gccaagaccg tgaaagcgaa
     4621 gctcaaagtc ttggatctcc tggaaaagca cgacgtcacg acgacattgc tgcccgcggt
     4681 cgcggcgggg ctcaacgacc gcgacgtggg cgcgctgttc gagctcatgc tctcgaagcc
     4741 caacatctgc tcgctcgagc tccacacgct gacgttcacg ggacagggcg gcgtcggctt
     4801 ctcgcgcggc gcgcgcatca cgacccctga ccttcatcga atcttggcgg aggcgactgg
     4861 cggcaggctg cgctggacgg acttcgtgcc ctcgccgctc gcccaccctc actgctactc
     4921 gatctgttac gtgctcatgc tcgacgatgg cggctacgtg ccgttcactc gattcatgag
     4981 ccgcgccgag ctgttcgagc tgctttcgga ttcgatctac attcagccgc gcgcaaatac
     5041 ggagcgcgtg ctaagaaact cgattgacga gctctgggcg aacccggact ccgtgcccga
     5101 agccgagcgc gtgctcgcca cgttgaagcg cctggtcggc gagctctttc cgagccgcgg
     5161 accgatttcc ctcgaagcac gccagaagat cgccgaacgc gccagtaagg ccatctatat
     5221 ccattcgcac atggatgagg agagcttcga cgtttcgcgc atcatgaagt gcagcgtcgg
     5281 cgttcccgag gtggacggct cgaacatccc gacgtgttcc tacaacgtgc tctatcgaga
     5341 gcgcgaccct cgcttcgcgg atgcttcgat gttgaagcga atgagcgact ttcggcctcc
     5401 ggcggcggtc gcggcgcgca gcttgccgat cgtgaagtga ggatgagcgc ggccccggct
     5461 ctcccttggg tcagtcgtgc ca
//
LOCUS       DASTKS010001244         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_201492, whole genome shotgun sequence.
ACCESSION   DASTKS010001244 DASTKS010000000
VERSION     DASTKS010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201492"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFQ35_26590"
     CDS             complement(<1..365)
                     /locus_tag="VFQ35_26590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934310.1"
                     /translation="MADRSNQAPRTRGANHPRRGATSAKVPQNLRRVAFLVALALGCS
                     SSKDESGGTGGGSSAAGQSGSEGGASGGSQSGGSAAGGSGATGNSSSGGASRGGNGAG
                     GGASSFGGRAPSGGAPNGGA"
     gene            complement(479..1942)
                     /locus_tag="VFQ35_26595"
     CDS             complement(479..1942)
                     /locus_tag="VFQ35_26595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934311.1"
                     /translation="MRLLGPSLCVALGLFFAGACVGPEFRSGGGAGRSGGGGGGLGSG
                     GSAAASGGSVGSGGGENTGGARSGCAEHADCDDSDSCTVDRCTEDGTCLNEAKCVSGQ
                     KCCGGVCGQCCSASDCDDGVACTQDNCKNSVCVHVPNDGACSDLQYCSAVDGCRARVR
                     CTGDFDCDDGDPCTTETCIDNQCVRSGCGADKLCCPGQGCATCCNDSQCNDGDPCTKD
                     RCDGGKCTAGALCEGTLSCCPSADGKTATCGTCCSAADCDDRVSCTVDLCTTTGCAHV
                     ANSSVCSSGEVCNPTAGCQPSVQCSSPAQCQTQDPCVTPRCSSGKCDYTSCPVGQSCC
                     KDGCKACCSELDCADLYDCTVDSCSNEGCQHQPSDSKCSPGFRCVPEAGGCVECTRDA
                     DCDDKSPCTQDSCVKNRCVHTNACGARGQLCCGDRCAACCSAYDCVPAVLPGGKPGSC
                     TSYSCVDGACTSKTVATCLGTCCPVGGCQLAGCSQAQ"
     gene            2167..2526
                     /locus_tag="VFQ35_26600"
     CDS             2167..2526
                     /locus_tag="VFQ35_26600"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9934312.1"
                     /translation="MSSTSAMKGPQQSSDPLYLLLRDEQVLEFNRRRPAGSACDMRGL
                     SFRGRDLRNLEAGGIDFGDAYFRDADLRGIDFRGANLEGASLASAKISGAYFPEAISA
                     EEVRLSVEYGTRLRYKK"
     gene            complement(2542..3345)
                     /locus_tag="VFQ35_26605"
     CDS             complement(2542..3345)
                     /locus_tag="VFQ35_26605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934313.1"
                     /translation="MTILCARLLARLPKLGALGALVGVGLAGVAGCGDAQHSLGGVTA
                     QNRDDPSAVDHVGRCEPGAAVDMIDNMEDGDGSIFLSHGRAGVWFSYNDDTNKDVGSG
                     TTQWPKAGAERFFMDPVSPPRKGSIKAAHTNGSGYSNWGAGIGFDLLVQQPYDASRYR
                     GLTFFLRRAPEADAAVRLNVTDRNTSPLGGVCDADHNGCYSDFGIDLQATESWQEVKL
                     EWSELTQPSWALMNYDAIATSALYGIRFQSKPNAHFDFWIDDIAFLCAE"
     gene            complement(3305..4225)
                     /locus_tag="VFQ35_26610"
     CDS             complement(3305..4225)
                     /locus_tag="VFQ35_26610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934314.1"
                     /translation="MSDLSRLLESSEDQDALSLLRAAHEQPPEGSMGRVAAALGVGVG
                     GALVSGASATALLSSKAHAASSLLGASKVGAEVAVSSTPWLASVIAKPLAIGLLGGLA
                     AVSGVNYVSSSPARSSEQRQVPSSVTRAPVARPAHTGTPPRAAPPVAVELSEAPTPKV
                     EPQQRASAPASQARRHAVAAAPHEVVAPPATATVEAPTKTSEAPPVAKQAAPPPRDEA
                     LVRELALLGRARSQLVAGNAAGALRVLDEYSAERRSGALEPEAVTLRIQALERVGDRA
                     SAARLARSFIKAHPERAHDDSLRSIASEAP"
     gene            complement(4222..>4522)
                     /locus_tag="VFQ35_26615"
     CDS             complement(4222..>4522)
                     /locus_tag="VFQ35_26615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9934315.1"
                     /translation="EVFDEAMAEQEKDPLPGADRLLSQKRLRQLFDQILSSWTPDIRG
                     TFLLFEIEGLSVPEIAEVTNVPVGTVSSRLRRGRELFLAGVKRLKARGLLDGESL"
BASE COUNT          751 a         1589 c         1434 g          748 t
ORIGIN      
        1 gcgccgccgt tcggcgcgcc tccgctcggc gctcggccac cgaagctcga agcgccgccg
       61 cccgcgccgt tgccgcctcg cgacgctccg cccgaactcg agttgccggt cgcgccgctt
      121 ccccccgccg ccgagcctcc gctctgcgat cccccgctcg cgccgccttc cgagccgctt
      181 tgccccgctg cgctcgagcc gccaccggtc ccaccgctct cgtccttcga cgagctgcaa
      241 cccaaggcca gcgcgaccag gaacgcgacc ctcctgaggt tttgcgggac cttcgcggaa
      301 gtcgcgccgc ggcgcggatg atttgctcct cgagtgcgag gcgcttggtt cgagcggtct
      361 gccatgctga gtcctctcgc caggatttag gggcggcagt tcgatttttg gggccgattt
      421 cgtggcatct tcaatcacgc cggtgaccgc tcgaacgggc acggcctcgg attcgctcct
      481 actgcgcctg tgagcagcca gcgagctggc aaccgccaac gggacaacag gtccccagac
      541 aggtcgccac ggtcttgctc gtgcacgcgc cgtcgacaca gctgtacgag gtgcaggagc
      601 cgggcttgcc tccgggtaac accgccggca cgcagtcgta ggccgagcaa cacgccgcgc
      661 agcggtcgcc gcagcatagc tgcccacgcg ctccgcaggc gttcgtgtgt acgcagcggt
      721 ttttcacgca actgtcctgg gtacacgggc tcttgtcgtc gcagtccgca tcccgggtac
      781 actcgacgca tccccccgcc tccggaacgc agcggaagcc agggctacac ttgctgtccg
      841 aaggctggtg ttggcagccc tcgttggagc acgagtccac cgtgcaatcg tagagatcgg
      901 cgcaatcgag ctccgaacag cacgccttgc acccgtcctt gcagcagctc tgccctaccg
      961 gacagctcgt gtaatcacac tttccggacg agcacctcgg agtgacgcac ggatcttggg
     1021 tttggcattg ggcgggcgat gagcactgca cgctcggctg acagccggcg gtgggattac
     1081 atacctcgcc gctgctgcac acgctcgagt tggcgacgtg cgcgcaacca gtcgtggtgc
     1141 agagatccac cgtgcacgac acgcgatcat cgcaatcggc ggcgctgcag cacgtcccgc
     1201 acgtcgccgt ctttccgtcc gccgaagggc agcacgagag tgtgccttca cagagcgcgc
     1261 ccgcggtgca cttgccaccg tcgcagcgat ctttggtgca gggatcgccg tcgttgcact
     1321 gcgagtcgtt gcagcacgtc gcgcaccctt gccctgggca acatagcttg tcggcgccac
     1381 aaccgctccg aacgcactgg ttgtcgatgc aggtctcggt cgtgcaggga tcgccgtcgt
     1441 cgcagtcgaa gtcacccgtg catcgcacgc gcgcgcgaca gccgtcgacg gcggaacaat
     1501 attggagatc gctgcacgcg ccgtcgttgg ggacgtggac gcaaaccgaa ttcttgcagt
     1561 tgtcttgcgt gcacgcgacg ccgtcgtcac agtcgctcgc cgagcagcat tggccgcaga
     1621 caccgccgca gcatttttgg ccgctcacgc atttggcctc gttcaggcag gtgccgtcct
     1681 ccgtacagcg atccaccgtg cacgagtccg agtcgtcgca gtcggcgtgc tcggcgcagc
     1741 cgcttcgtgc gccgccagtg ttctctccac caccgctgcc aacgctgccg ccggacgccg
     1801 ctgccgaacc gccgctgccg agaccgccgc ctccgccgcc gcttcttcca gcgcccccgc
     1861 cgcttctgaa ctcaggccca acgcaggcgc cagcaaagaa gaggccgagc gcgacgcaaa
     1921 gagaagggcc gagaaggcgc aatgaaatga ctccgcgggc gactcgagcc gcctctcacg
     1981 aacctattcc gcccggcgat cgctgtcgag taggtatcag aagcacgcac cggagcgcac
     2041 acccgtcgcg ctcggaagct ccggcgaaag cgagcgacac ggcgcgaacg taccgatgtt
     2101 gccgtacaga ctgcgagctc tgaactcttt tcccttaaag tgtgcgcgcc atctcgccgt
     2161 tctctcatgt cgagtacaag cgccatgaaa gggccgcaac aatcgagcga tcccctctac
     2221 ctcctactcc gcgacgagca ggtgttggag ttcaatcgcc ggcgccccgc ggggagcgcg
     2281 tgcgatatgc gagggctctc gttccgcgga cgtgacctca ggaacctcga ggcgggcggc
     2341 atcgatttcg gggatgcgta ctttcgtgac gccgacctgc gcggcatcga ttttcgcggc
     2401 gcgaacttgg aaggcgcgag cctcgcgtcg gcgaagatct ctggagctta cttcccggaa
     2461 gcgatctcgg cagaagaggt tcgcctcagc gtcgagtacg gcacgcgcct gcgctacaag
     2521 aagtgagaga gagcgcgcgc gtcactcggc gcacaagaac gcgatgtcgt cgatccagaa
     2581 gtcgaagtgc gcgttgggct tcgactgaaa tcgaatgccg tacaacgccg aggtcgcgat
     2641 cgcgtcgtag ttcatcaatg cccagctcgg ctgcgtcagc tccgaccatt cgagcttgac
     2701 ctcttgccag ctctcggtcg cctggaggtc gatgccgaaa tcgctgtaac acccgttgtg
     2761 atcggcgtcg caaacgcccc caaggggtga ggtgttgcga tccgtcacgt tgagtcgcac
     2821 cgccgcgtcc gcctctggcg cgcggcgaag aaagaaggtg aggcctcggt aacgggaagc
     2881 gtcgtaaggc tgctgcacca gcaagtcgaa gccgatgccg gcgccccagt tcgagtaacc
     2941 gctcccgttc gtatgcgccg ccttgatgct gcccttgcgc ggcggtgaca cgggatccat
     3001 gaagaaccgc tcggcgccgg cctttggcca ctgcgtcgta ccgctcccca cgtccttgtt
     3061 ggtgtcgtcg ttgtaggaaa accaaacccc agcgcggcca tgactgagga agatggaacc
     3121 gtcgccgtcc tccatgttgt cgatcatgtc gacggcagcg ccgggctcgc agcgaccgac
     3181 gtgatctacc gcgctcggat cgtcgcggtt ctgggccgtg acaccaccca gtgaatgctg
     3241 ggcgtcgccg cagcccgcga ccccggcgag ccccaccccc acgagcgcgc cgagcgcgcc
     3301 gagcttaggg agcctcgcta gcaatcgagc gcagagaatc gtcatgggcc ctctcgggat
     3361 gagccttgat gaacgaccgc gcaagtcgag ccgcggaagc ccgatccccg actcgctcga
     3421 gcgcctggat gcgcagggtg accgcctctg gctccagcgc tccgctgcgg cgctcggccg
     3481 aatactcatc gagcactcgc agcgccccgg ccgcgttgcc ggcgaccaac tgactcctcg
     3541 cccggccgag cagcgcgagc tcgcgaacga gcgcctcgtc ccgcggaggc ggagccgctt
     3601 gcttcgcgac gggcggagcc tcgctcgtct tggtcggcgc ctcgacggtc gccgtcgcgg
     3661 ggggagcgac gacttcgtgc ggcgccgccg caaccgcgtg acgccgggcc tgcgatgccg
     3721 gcgcactcgc tcgttgttga ggctcgacct tcggcgttgg cgcctcgctg agctcgaccg
     3781 caaccggagg agcggcgcgc ggcggcgtgc ccgtgtgcgc tggcctcgcg acgggagcgc
     3841 gcgtgacact cgaaggtact tgccgctgct cgctcgagcg cgctgggctg gagctcacgt
     3901 agttcacgcc gctaaccgcc gcgagaccgc ccaaaagccc gatggcgagc ggcttcgcga
     3961 ttaccgaagc gagccaggga gtcgaagaga ccgccacctc cgcgccgact ttgctcgcgc
     4021 cgagcaacga tgatgccgcg tgcgccttcg acgaaagcag cgccgttgcc gaggcaccgg
     4081 agacgagcgc gccgccaact ccgacgccga gcgccgctgc gacgcgaccc atcgagcctt
     4141 ccggcggctg ttcgtgcgcg gcccgaagca acgacagcgc atcctgatcc tcgctgcttt
     4201 cgaggaggcg agacaaatcg ctcataggct ctctccgtcg agcagcccgc gcgctttcaa
     4261 gcgtttgacg ccggccaaaa agagttcacg cccacggcgc agccgcgacg acacggtgcc
     4321 aaccggaacg ttcgtcactt cagcgatctc cggcacgctc aacccctcga tctcgaagag
     4381 caggaaggtg ccgcgaatgt cgggtgtcca cgagctcaag atttgatcga acagttggcg
     4441 gaggcgcttc tgcgagagga ggcgatcggc gcccgggagc ggatccttct cctgctcggc
     4501 catcgcctcg tcgaacacct cg
//
LOCUS       DASTKS010001245         9005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_870289, whole genome shotgun sequence.
ACCESSION   DASTKS010001245 DASTKS010000000
VERSION     DASTKS010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870289"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFQ35_26620"
     CDS             <1..626
                     /locus_tag="VFQ35_26620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934316.1"
                     /translation="TLADDLARSNRRDGLQITDPKHPSYGAFLATAAARSSGATSLDD
                     QGIRLWALRIAYERTHAVKYLRSAEALVNHFLRVRGEDHLFTGTVFVDERFREAGLSQ
                     GRTPLGQYALLAGLKAWSDLVPRARQLYAAGLEHATRRHGVHSVGWSGPYRMIFPREG
                     VFDFGADTELGGSFLWALSLDSSSLRGRFPAQCRRSPNAPDPTEGYP"
     gene            623..4438
                     /locus_tag="VFQ35_26625"
     CDS             623..4438
                     /locus_tag="VFQ35_26625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934317.1"
                     /translation="MSIRLQARPAHWLTLSVMLLGSAPALAAPSGERLPPSAADSLTA
                     SRTALRRWLERGESAEARRGGKVALASREYEVTDAKLLESWLAWLPDFSLALRRQYDR
                     TSSGNVYTPRWRVDVEGQASFSLQKFAAASAARAARAKAQAEVAQARHVARQATLFRV
                     VELYLAERKALPLRAQLDDFERLASSLAGARGDGDGDAILLNARLAELRGELAKVERE
                     RRDAAERLGAELDAPVEASGIDPRLGATELIELIGATMSASPSRTDDVLRAELDLQRE
                     RLRFTQSQAWYQPQLRANGVAQFPKSDPGAVNGDNFRLAGITAEFSLGLRVRPGIPSL
                     ETAAEQAVLRSEFEGAQSRWKRGQLADRARAIRDALAQLWGEQRGVSAARTSYEDITQ
                     RFMRGERTATELSASSRSLLETELERDAILEQALIAQIVLSSDSEGGRATPTNSSAPI
                     PSAEANQLVLRAASDAAAVKAALAEARRAQQNARGESFPLQTSVDAGVSVPLYENSAL
                     NVESRPLLAFAGTSTLTTPVREVSALGRWTVDLRGVGTTASAAAREAELRAAELTLTR
                     KRYLWAALSARLELAHARMQLTAAERAVEVTSGRVALERRWLQQGAANDEDLRNAELA
                     QRNALLARGRAEARERAAALLVGSYLGAPRGTPLSVDETPHVLEAWAREHLFPEQQLH
                     GFEGALAERVALLEVEALRSRTEALARPPRATTFAAQATQGVHGGAFSLTVALSVSLD
                     PLRDPPQATRAAQAEGAARGRLLALQRRLEQQRTRLQERIAAATRELETERDNAQRLS
                     RLEATLRDEQAALPDVHDAVRERGRAALETARIQSELRRLDAEERLRSASLEVLALGA
                     NATTRTPTGAAPAPSPSLNAALAILRDERSDVMVADAVAREVGVHSRAVPVASALHLV
                     GPFSVGSYSATRVTGPSTTKILQADQGIGLSLALDEAVSFGSLAKLRQSAQLEQKAAR
                     RRTEREAVREVAEAWTARELERLSRAEEAAAQHYVEDSVAPRFALGQVTPGLLTSAEV
                     ERTRARSQHAADDTAFRAARAALAAQGVTLDDALLDDFQRRASTALPTESTGRQPVAS
                     ASPDELAAQARSAAGSYDVAASGLRLLSPVTGLLEFRPARIDFSSGTGDARVTSSDRE
                     QLWVLSLLVPLRLQAIGRLSVDMARSRLAEEELGTASREARAESLRLARAVVLSRNAH
                     AAAVAERESVERALAEVERRFRNQERRTSIDEVVQARKALFEARRSEILAAGAAFEAV
                     WSLQTAGSP"
     gene            4435..5685
                     /locus_tag="VFQ35_26630"
     CDS             4435..5685
                     /locus_tag="VFQ35_26630"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HET9934318.1"
                     /translation="MKLLKVPTSLGVSCTLTMMCAAGCLSAEVENPCNSSPTFERFSE
                     EAAKLAAASYVPEEVQPCPPLDRPPDGDKSAEGVVFEEYAEIISGTSGNFLDQSSWWA
                     KALPRGELHVQTLQIDDAACRPLSYEQFSYDYAAATPPIVPTPQCGHSLSGVAMGHQF
                     FQSSPQIFELEATAYARLVGLQVNPVLGTSLRFATENAAYEGEQFPALTRVAIASPDE
                     HALGFAARIRATSFEGAMEGKLTAAAATTLRIRLRVFPRAGADPTIRLGVLAMSSMFW
                     KGADDTPEVTDDQAHDTDHLYVRMQDGSEQLIPLINPPPELQYEADPWQRTTFDGPLE
                     SIGLEQKERDPTRFLRYRSAHYSERPSVTASEITSNVPLTAELSVSYANSEYFDNLVL
                     NLVAHPDGSGAPIEVSYLLTASSI"
     gene            complement(5717..6904)
                     /locus_tag="VFQ35_26635"
     CDS             complement(5717..6904)
                     /locus_tag="VFQ35_26635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934319.1"
                     /translation="MNFAKRFAAVTTLLLCTGCVYEKGKEKPCANAPTLEDRKNDARM
                     AAGSEYVPPLTAACPELDPPVDGESDTPGLSELEYQALLRGANGNFFDDGTWWIKAMP
                     RGGVHVSPLKIVGERCQEVTYDRFGYDYAFAKPAITAAPQCGHYLTGILAGHKFGDSW
                     PAIFGVEASGLIRLAPLSPQLAFDAYLHAPRGLVGTSTEASGIDRVDVELHDGDSVEF
                     AMLVSATTFTSALRVVLKAGDESRLAVGVDVYPRANFQAAPRIALGGLSGAYHEDAAH
                     DFDRMEVTYSDGTTHEAKLDDGSLEWGNGDWARVPSFAGTAGVQSIGLLQSAAPGGGP
                     RSPKMTISNIDSNVPIGFELALTSRQVLGGNVVGSLVLDLSEAGAFESPIHVSYLVTA
                     AAP"
     gene            complement(6901..8073)
                     /locus_tag="VFQ35_26640"
     CDS             complement(6901..8073)
                     /locus_tag="VFQ35_26640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934320.1"
                     /translation="MALRGLRAFRVSPNGRGHVAGDNTGRYGRAWTKCTNLALDLLTE
                     LVAERSSLQGARITAENVSDVLAVLEQLRTFHGIFPEFIKLEGSVHAEVKAGAVRYSS
                     IDSAWVTVALSIAEARYRAERPDLAQRASELLRRQDYSVLVEGRRLLGFVSIDALSGA
                     VLERARFAYGDRNSEARPLVLALTGLGKLPASIWDEMRYSWTLRDGLPVASGYGASAF
                     VELSGQLFFDEMTLAPKSLGLSHENYVKASAQVAHQRHHSIWGYAPSCAASERYAEFG
                     LDNPDVVAPYAAALLATTRVPLAASNLERVFAALDWSGAPFADSLDPVSQRALCAEAR
                     MLDQSLLFLSLNVDALRELSRRTSWYASAEARLRDMDRTHAPELNSVAVSSEVYRK"
     gene            complement(8271..>9005)
                     /locus_tag="VFQ35_26645"
     CDS             complement(8271..>9005)
                     /locus_tag="VFQ35_26645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9934321.1"
                     /translation="TPSDFARPAGSARKESCGSVSRLLQSGSGEGKARDDDRVVLDYA
                     AFTSEGQPLDSSAAHGEPLTQSVRNLPPGLVCVVKRMQVGESRRVWVPARLMHTGKGE
                     ASDEVKTDRTIDITLRGLTRAPARPEDYAAPPRAAQRTPSGLRFRILQRGKAEERPTP
                     NSRVTVYHSGWTSRGELFESSTLAGHPASYIVYELPQGLAEGLQLMHVGDKARFWLPE
                     RLAYARTNRGAPKGPVVFDVELLSIE"
BASE COUNT         1555 a         3077 c         2908 g         1465 t
ORIGIN      
        1 tgacgctggc ggatgacttg gcgcgctcga accggcgcga tggcctgcag atcacggatc
       61 cgaagcaccc gagctacggg gcgttccttg cgaccgcggc ggctcgttcg agcggcgcga
      121 cctcactcga cgaccagggt atccggctgt gggcgctccg catcgcgtac gagcgcactc
      181 acgccgtgaa gtaccttcgc tccgcggagg cgctcgtgaa tcacttcctg cgcgtgcgcg
      241 gcgaagacca cctgttcacc ggcacggtgt tcgtcgacga gcgtttccgc gaggccgggc
      301 tctcgcaggg gcgaacacct ttgggccaat acgccctact cgccgggctc aaggcctggt
      361 ccgacctcgt gccgcgagcg cgacagctct acgcggcggg gctcgagcac gcgacgcgac
      421 gccacggcgt tcatagcgtc ggttggagcg gtccctatcg tatgattttc ccacgtgaag
      481 gagtcttcga cttcggcgca gacacggaac tcggcggatc gtttctgtgg gcgctgtcgc
      541 tcgactcgag ctcgcttcgt ggccgatttc cggcccagtg ccggcgatcg ccgaacgcgc
      601 cggaccccac cgaaggatat ccgtgagtat tcgtctgcaa gctcgtcccg cccattggtt
      661 gaccctgtct gtcatgctac tcggcagcgc cccggcgctc gccgcaccga gcggagaacg
      721 cctgcccccg agtgcagccg attcgctcac cgcgtcgcgc accgcactgc gtcgctggct
      781 cgaacgaggc gagagcgcag aggcgcgccg cggcggtaag gttgcgctcg cctcccgtga
      841 gtacgaggtc accgacgcaa aactcttgga atcttggctc gcgtggcttc ccgacttttc
      901 tttggcgctg cgccggcagt acgaccgcac gagcagcggc aacgtttaca cgccgcgctg
      961 gcgagtcgac gtcgaaggac aagcctcctt ttcgctccag aagttcgccg ccgcgagcgc
     1021 tgcgcgcgcg gctcgcgcca aggcccaggc cgaggtggca caggcgcggc acgtcgcgcg
     1081 acaggccacc cttttccgcg tcgtcgagct ctacctcgcg gagcgaaaag cgcttccgct
     1141 ccgcgcacag ctcgacgact tcgaacgcct cgcatcgagc ctggcgggag cgcgcggaga
     1201 cggcgacgga gacgcaatcc tgctcaacgc gcgcttggcc gagcttcgcg gtgaactcgc
     1261 caaagtcgag cgtgagcgtc gcgatgcagc cgagcgcctc ggcgcagagc tcgacgcacc
     1321 ggtcgaggcg agcggaatcg atccgcggct cggcgcaacc gagctaatcg agctgattgg
     1381 cgcaaccatg tccgcttcgc cgtcgagaac cgacgacgtc ttacgcgcgg agctcgactt
     1441 gcagcgagag cgcctgcgct tcacccagag ccaagcgtgg tatcagccgc agctgcgcgc
     1501 gaacggcgtc gcccaattcc cgaagagcga tcccggcgca gtgaacggag acaacttccg
     1561 cttggcggga atcacggctg agttttcgct cggcttgcga gtgaggccag gaattccttc
     1621 actcgagact gcggccgagc aagcggtcct ccggagcgag ttcgagggtg cgcagtctcg
     1681 ctggaagcgc ggacaactgg ctgaccgtgc gcgggcgatt cgcgatgctc tcgcgcagct
     1741 ctggggcgag cagcggggcg tctccgccgc tcgaacgagc tacgaggata tcacccagcg
     1801 cttcatgcgc ggcgagcgaa ccgcgactga gctgtccgct tcgagccgaa gcttgctcga
     1861 aaccgagctc gaacgcgacg ccatcctgga gcaggcgctc atcgcccaaa tcgtgctttc
     1921 gtcggattcg gagggcgggc gcgcgacgcc gacgaactcg agcgcgccga tcccttcggc
     1981 agaggccaac cagctcgttc ttcgcgccgc gagcgacgcg gccgcggtca aagctgcgct
     2041 cgccgaagcg cgccgggccc agcaaaacgc ccggggcgag tcctttcctc tccagacctc
     2101 ggtcgacgcc ggggtatcgg tccctctata cgaaaactcc gccttgaacg tcgagtcgcg
     2161 tcccttgctc gccttcgccg gtacgagcac gctgacgacg ccagttcgag aggtgagcgc
     2221 gctcgggcgc tggacggtcg atttacgcgg ggtgggaacg acggcaagcg ccgctgcacg
     2281 cgaagccgag ctacgcgccg ccgagctcac cctgacgcga aaacgctatc tgtgggcggc
     2341 cctgagcgcc cgactggagc tcgcccacgc gcgcatgcag ctcaccgccg ccgagcgggc
     2401 cgtcgaggtc acttcgggtc gggtggctct cgagcgccgt tggttgcaac agggcgctgc
     2461 aaacgacgaa gatctgcgga acgccgagct cgcgcaacgt aacgcactgc tcgctcgcgg
     2521 acgagccgag gcgcgagagc gtgcggccgc gctcctcgtc ggcagttatc tcggggcgcc
     2581 acgcggcacg ccgctttcgg tggacgaaac gcctcacgtg ctcgaagcgt gggctcggga
     2641 gcatctcttt ccagaacagc agctgcacgg tttcgagggc gctctcgcgg agcgcgtcgc
     2701 gcttctggag gtcgaagctt tgcggtcacg taccgaggcg ctggcgcgac caccacgcgc
     2761 gacgacgttc gccgctcagg cgactcaagg cgtacacggc ggcgctttca gcttgacggt
     2821 cgccctgagc gtatcgctcg acccgctgcg tgatcccccg caggcgaccc gcgccgctca
     2881 ggcggaggga gcggcgcgag ggcgcttgct ggctctccag cgtcgcctcg aacagcagcg
     2941 cacgcgcctg caggagcgca tcgccgccgc gacccgagag ctcgaaaccg agcgcgacaa
     3001 cgcgcagcgg ctgtcccggc tcgaggcaac gctgcgagac gaacaggcag cgctccccga
     3061 cgtgcacgac gcagtcagag agcgcggacg agccgcgttg gaaaccgcgc ggatccaatc
     3121 cgagctgcgc cgcctcgacg cggaagaacg cctccgctcg gcgtcgttgg aggtgctggc
     3181 gctcggtgcg aatgcaacga cccgcacacc aacgggggcc gcgcccgctc cgtcaccgtc
     3241 cctgaacgca gctctcgcta ttttgcgcga cgagcggagc gacgtcatgg tggcagacgc
     3301 agtggctcgc gaggtaggcg tgcacagtcg agccgtccct gtcgcctcgg ctttgcactt
     3361 ggtcgggccg ttcagcgtcg gttcgtactc ggcgacgcga gtcactgggc cttcgacgac
     3421 caagatcctt caggccgacc aaggcatcgg tctgtcgctc gccctcgacg aagcggtctc
     3481 gttcggatcg ctcgcgaagc tccgacagtc ggcgcaactc gagcagaaag ccgcacgacg
     3541 ccgaaccgag cgcgaagccg ttcgggaagt cgcagaggcc tggacggctc gggagctcga
     3601 gcgactctct cgcgccgagg aagccgcggc gcagcactat gtcgaggact cggtcgcgcc
     3661 tcgcttcgcc ctgggccaag tcactcctgg gttgctcacg agcgcggagg tcgaacggac
     3721 acgcgcgcgt tctcagcacg cagccgacga cactgcgttt cgcgcagctc gcgcggcgct
     3781 cgcggcccag ggcgtcaccc tggacgacgc cctgctcgac gactttcagc gtcgagcgag
     3841 cacggccctg ccgaccgagt ccaccggacg ccagcccgtc gcgagcgcta gtcccgatga
     3901 gctagcggca caggcccgca gcgctgccgg ttcgtacgac gtggcggcga gcgggctgcg
     3961 cttgctttcg cccgttactg ggctgctcga gttccgtccg gcgcgcatcg acttcagcag
     4021 cggaaccggt gacgcgcgcg tcacctcgag cgaccgagaa caactttggg tgctctccct
     4081 cctcgtacca ctgcgccttc aagcaatcgg ccgactttcg gtcgacatgg cgcgttctcg
     4141 cttggccgag gaggaactcg gaaccgcgtc tcgcgaagct cgggccgaaa gccttcgcct
     4201 cgcgcgcgcg gtcgtgctct cccgaaatgc tcacgcagct gccgtcgccg agcgcgaaag
     4261 cgtcgagcgc gctcttgctg aagtcgagcg ccgcttccgc aatcaagagc gccggacatc
     4321 catcgacgag gtcgtgcaag cccgcaaagc gctgttcgaa gcgcgacgca gcgaaatcct
     4381 cgccgcgggc gcagcgttcg aggcggtctg gtcgctgcag acggcgggtt ccccatgaag
     4441 ctactcaagg ttcctacttc gctcggcgtt tcgtgcacgt tgaccatgat gtgcgccgcc
     4501 ggctgcctct cggccgaggt agagaacccg tgcaattcga gcccgacttt cgagcgtttc
     4561 tccgaagaag cagcgaagct agcggcggcg tcgtacgtgc cagaagaggt gcagccgtgt
     4621 ccgccgctcg atcggccgcc agacggcgac aagagcgcag aaggcgtcgt cttcgaggaa
     4681 tacgccgaga tcatctcggg cacctcgggc aactttttgg atcagagctc gtggtgggcc
     4741 aaagcgctgc cgcgcggcga gctccacgtg caaactctgc aaatcgacga cgccgcgtgc
     4801 cgtcccctct cttacgaaca attcagctac gactacgccg ccgcgactcc gcccatcgtc
     4861 ccaaccccac agtgcggaca cagcctctcc ggcgtcgcga tggggcacca gttcttccag
     4921 tcgagcccac aaatcttcga gctcgaggcg acggcgtacg ctcgcctcgt cgggctccag
     4981 gtaaatcccg tactcggcac ttcgctgcgt ttcgcgaccg aaaacgcggc ctacgaaggc
     5041 gagcaatttc cggcgctgac gcgcgtagcc atcgcgtcgc ccgacgagca cgcgctcggc
     5101 tttgcggcgc gcatccgtgc gacgtccttc gagggcgcca tggagggcaa gctcaccgct
     5161 gccgccgcaa cgaccctacg cattcggtta cgagtcttcc cgcgcgcagg cgcggatccg
     5221 acgatccgct tgggggtgct cgcgatgagt tcgatgtttt ggaagggcgc cgatgacacg
     5281 cccgaggtca ccgacgacca agctcacgac acggatcatc tgtacgttcg catgcaagac
     5341 ggcagcgagc agctcatccc actcatcaat cctcctcccg agctccagta cgaggcagac
     5401 ccttggcagc gcaccacctt cgacggtccg ctcgagagca tcgggctcga gcagaaagag
     5461 cgcgacccga ctcgcttctt gcgctaccgc tctgcgcact actcggagcg accttcggtg
     5521 acggcgagtg agatcacgtc gaacgtcccg ctcacggcgg agctctctgt gtcgtatgcc
     5581 aacagcgagt acttcgacaa cctggtgctc aacttggtcg ctcacccaga cggctcgggc
     5641 gcgccgatcg aggtgagcta cctgctcacg gcgtcgtcga tctgagccga acctcgccgc
     5701 gcggcgagca cccgtctcac ggagcggccg cggtgaccag gtaagataca tggatagggc
     5761 tctcgaaagc gcccgcctcc gacaggtcga ggactaggct cccgacgacg ttgccgccga
     5821 gcacttgacg actcgtcagg gccagttcga agccgatcgg cacgttggaa tcgatgttcg
     5881 agatggtcat cttcggcgaa cgcggacccc cgcctggcgc ggcgctctgc aacaggccga
     5941 tcgactgaac cccagccgtc cctgcgaaac tcggcaccct tgcccagtcg ccgttgcccc
     6001 actcgagaga gccgtcgtcg agcttcgctt cgtgcgtcgt cccgtcggag taggtgactt
     6061 ccattcgatc gaagtcgtgc gcggcgtcct catgatacgc gccgctcagc ccgccaagcg
     6121 cgattcgcgg tgcggcttgg aagttggcgc gcgggtaaac atcgaccccg accgcgagac
     6181 gcgactcgtc gcccgccttc aggaccacgc gaagagcgct ggtgaacgtg gtcgcgctga
     6241 ccagcatcgc gaattcgacc gagtctccgt catggagctc gacgtcgact cggtcgatcc
     6301 cagacgcttc ggtcgacgtc ccgacgagac cgcgcggcgc gtgcaagtag gcgtcgaacg
     6361 ccaactgcgg cgagagcggc gcgagccgga tgaggcccga agcctcgaca ccgaagatcg
     6421 ccggccacga atcgccgaac ttgtggccgg cgagaatgcc ggtcaggtag tgcccacatt
     6481 gaggggccgc cgtgattgcc ggtttggcga aagcgtagtc gtaaccgaaa cgatcgtacg
     6541 tcacctcttg gcaacgctcg cccacgatct tcaggggact cacgtgcaca ccccctcgcg
     6601 gcatcgcctt gatccaccac gtgccgtcgt cgaagaagtt gccgttcgcg cctcgcaaca
     6661 acgcctggta ctcgagctca ctcaaacccg gcgtgtccga ctctccatcg accgggggat
     6721 cgagctcggg gcacgcggcg gtgagcggag gcacgtactc gctaccagcg gccattcgag
     6781 cgtcgttctt gcgatcctcg agcgtcggag cgttggcaca gggcttctcc tttcccttct
     6841 cgtaaacgca acccgtgcac aggagcaacg tcgtgaccgc ggcgaatcgc ttcgcgaaat
     6901 tcattttcgg tagacctccg agctgacggc gaccgaattc agctccggcg cgtgcgtgcg
     6961 atccatgtct cgcaatcggg cctccgcgga ggcataccaa ctcgtcctcc gcgacaactc
     7021 gcgtaacgca tcgacgttga gcgacagaaa gagcagcgac tgatcgagca ttctggcttc
     7081 ggcgcaaagg gcgcgctggc tgactggatc gagcgaatcg gcaaacggtg cgccgctcca
     7141 atcgagcgcc gcgaacacgc gctcgaggtt gctcgctgca agcggaacgc gtgtcgtggc
     7201 aagcagggcg gcggcgtagg gcgcgaccac gtcggggttg tcgaggccga actccgcgta
     7261 gcgctccgag gccgcgcaac tcggcgcgta gccccaaatc gagtggtgtc gttgatgcgc
     7321 aacctgagcg ctcgccttca cgtaattttc gtgactgaga cccagagact tcggcgcgag
     7381 cgtcatttcg tcgaagaaga gctggccaga aagctcgacg aaagcgctag cgccgtagcc
     7441 cgacgccacc ggcagtccgt cgcgcaacgt ccaggagtac ctcatctcgt cccaaatcga
     7501 ggcgggaagc tttccgagcc cggtcagcgc gagcactagc ggacgcgcct cgctgttgcg
     7561 gtcgccgtac gcgaagcgcg cccgttcgag cacggcaccg ctcagcgcgt cgatggacac
     7621 gaagccgagc aagcggcgcc cctcgacgag cactgagtag tcctggcggc ggagcaattc
     7681 gctcgcgcgc tgggccaagt caggccgttc cgcacggtat cgagcctccg cgatactgag
     7741 cgcaaccgtg acccatgccg aatcgatcga cgagtagcgg accgccccgg ccttgacctc
     7801 ggcgtgaacc gagccttcga gcttgatgaa ctccggaaag atgccgtgaa acgtgcgcag
     7861 ttgctcgagg accgcgagca cgtcgctcac gttctcagcg gtgatgcgcg cgccctgcag
     7921 cgacgaacgt tcggccacca actccgtcag taggtcgaga gccaagttgg tgcacttggt
     7981 ccaggctcga ccgtagcggc ccgtattgtc gccggcgacg tgtccgcggc cattgggtga
     8041 gacgcgaaag gcgcgtaggc ctcgcaacgc cactgccgct cggcgtggac tctcgcggct
     8101 ctccgctcgc gccgcgtgct cgggcggctc ggcggaagcg tctctcaaac gacagctgct
     8161 aagcggcaaa gtcccgacta cgctcgcgag tgtagcaacg cttctgatgg cgatgagcct
     8221 gagtgcgcgc tgcgagatca cgatgaaggc ccattcgagg acattggtaa ttactcgatc
     8281 gacaggagct ccacgtcgaa gaccaccggg cctttgggtg cgccgcggtt ggtgcgcgcg
     8341 taggcaagac gctccggcaa ccaaaaacgc gccttgtccc ccacgtgcat gagctgcaag
     8401 ccctccgcga gcccttgagg tagctcgtag acgatgtagc tcgcggggtg tcccgccaag
     8461 gtcgaactct cgaacagctc gccgcggctc gtccaacctg aatgatagac ggtcacgcga
     8521 ctgttggggg tcggacgctc ctccgccttg cctcgctgca agatccgaaa acgaagaccg
     8581 ctcggagtgc gttgcgcggc tcgaggcggc gccgcgtagt cttctggacg ggctggggcg
     8641 cgcgtcagcc cgcgcagcgt aatatcgatg gtgcgatctg ttttgacctc gtcgctggcc
     8701 tcgccctttc cggtgtgcat caggcgagcc ggaacccaaa cgcgccgcga ctcgccgact
     8761 tgcatgcgtt tcacgacgca gaccaagccc ggcggcagat ttcggacgct ttgggtgagc
     8821 ggctcgccat gtgcagccga actgtcgagc ggttgtccct cggaagtaaa cgctgcgtag
     8881 tcgagcacga ctcgatcgtc gtcgcgcgct ttgccctctc cagaaccgct ctggagcaac
     8941 ctactgaccg agccacacga ctctttgcgc gcgctccccg ccggacgagc gaagtcgctc
     9001 ggggt
//
LOCUS       DASTKS010001246         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_201862, whole genome shotgun sequence.
ACCESSION   DASTKS010001246 DASTKS010000000
VERSION     DASTKS010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201862"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1316)
                     /gene="murE"
                     /locus_tag="VFQ35_26650"
     CDS             complement(90..1316)
                     /gene="murE"
                     /locus_tag="VFQ35_26650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HET9934322.1"
                     /translation="MGAASSVGVTGTNGKTSTASFIAEVLRCAGRRVVCETTVGSNTQ
                     PNEFVPPGAGPFGQRMQRFVDAGVEHFVLETTSEALAHGWAKRWRFDVGVFTNLSHDH
                     LNAHGSAEHYLASKAQLFVHLSPGGTAVLNANDVASTFIERVTPSTVRRLYFSAPSRG
                     ETLVPPTLALARCALDRDGTHIELLPGELADGLGRELHIRLVGEVFAENALAAALAGF
                     SFGLEPRVLKAGLFSLERVPGRFEVVVREPCVVVDYAHSEDALLRTCVAGRALLAQLG
                     GSKLIVVFGAGGDRDAEKRTPMGRAVGAHADVAIVTNDNPRSEDPQYIAQALLLGARS
                     GGRAECFVELERPRAIARALETAGKNDVVLICGKGHELYAVTDGDSAPRDDVDCVRRL
                     CGLGVSAQDSSHVAST"
     gene            complement(1324..2304)
                     /locus_tag="VFQ35_26655"
     CDS             complement(1324..2304)
                     /locus_tag="VFQ35_26655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934323.1"
                     /translation="MQISFDKFLKLTAVLAGSASIASACTIETVDGAKGGAAGASGQS
                     QGGLGGAHAGTGGVDAGAPSSEGGAPSAGAGGQTEGGAGGAPAMEGTGGAGGVIGGAG
                     GEGGAVACVSGAPAEEGLGFDCSTLSFAEARCPNPSGEGLPVPVPASEVCERYAAERQ
                     GSVDVLVRCLVKLSTADLCSATASARAAACERSMRESTCLASEAVAACTEIHERCDAI
                     GVESCSADLSPLSPETVTKVRDCTRASDSTQCAYTYGKCRGLPDQALNVSSVCAALTA
                     ECPALPLETCKATLDIYGTGMILDLTYYSYRDCIAKSTATCDAAFAACTD"
     gene            2572..2973
                     /locus_tag="VFQ35_26660"
     CDS             2572..2973
                     /locus_tag="VFQ35_26660"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9934324.1"
                     /translation="MLRVGWLFASVLSSCVVGCGGGNGASDAQLEKGRDMAATGALLY
                     ERNCQSCHGERGQGTSNGPAIFNKKKLGKRFKNAQRLFDYLAKEMPKDNPGSLDIAQY
                     WNIETFMVVASGRKLEDRLSESNAEDTKLDK"
     gene            2979..>3183
                     /locus_tag="VFQ35_26665"
     CDS             2979..>3183
                     /locus_tag="VFQ35_26665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934325.1"
                     /translation="MDRTAVFIDAGYLFASGARLLSNARASREELSLDNEALVATFTR
                     LARELTGLPMLRIYWYDASYGGPT"
BASE COUNT          575 a         1098 c          996 g          514 t
ORIGIN      
        1 aacccaagca tcggaacgac tctttcgaag cacgggtcgg aaccaactct ttcgtgccga
       61 ggcccactgc gagcgaacga ggcgacgccc tacgtgctcg cgacatgtga cgaatcctga
      121 gcgctcacgc cgagcccgca aagtcggcgc acgcaatcga catcgtcccg cggggcggag
      181 tcgccgtcgg tcaccgcgta gagctcgtga cctttgccgc aaatcaatac gacgtcgttc
      241 ttcccggcgg tttcgagcgc acgcgcaatg gcacggggcc gctcgagctc gacgaagcat
      301 tcggctcgcc ctccagaccg agcgccaaga agcaacgctt gcgcgatgta ctgcggatct
      361 tcactgcgcg ggttgtcgtt cgtcacgatc gctacgtctg catgcgcccc gactgcgcga
      421 cccatggggg ttcgcttttc ggcgtcgcgg tcgcctcccg caccaaacac cacgatcaac
      481 ttggaaccac cgagctgagc caacagcgcg cgacccgcaa cacacgttcg cagcagcgcg
      541 tcctcactgt gcgcgtagtc caccacgacg cagggctccc gcacgaccac ctcgaagcgc
      601 cccggcacgc gctcgagaga gaacaaaccc gccttcagaa cgcgcggttc gaggccgaat
      661 gaaaacccag cgagcgcggc cgcgagcgcg ttttcggcaa acacttcgcc gacgagtcga
      721 atgtggagct ctcgaccgag cccatcggcg agttcaccgg gcagaagctc gatgtgagtg
      781 ccgtcgcgat cgagagcgca gcgcgcgagc gcgagcgtcg gcggcacgag cgtctcgcct
      841 cggctcggcg ccgagaagta gaggcgccgc accgtgctcg gcgtgacgcg ctcgatgaaa
      901 gtcgaggcaa cgtcgttcgc gttgagcacc gccgtccccc ccggcgagag gtgaacgaag
      961 agctgcgcct tgctcgcgag gtagtgctcg gcagagccgt gcgcgttcaa gtgatcgtgc
     1021 gacaaattcg tgaaaacacc gacgtcgaac cgccagcgtt tggcccagcc gtgcgcgagc
     1081 gcctcgctcg tagtctcgag gacgaagtgc tctacgcccg cgtccacgaa gcgctgcatg
     1141 cgctggccga aaggtccggc gccgggcgga acgaactcgt ttggctgcgt attcgagccc
     1201 acggtcgttt cgcacacgac gcggcggccc gcgcagcgca acacctcggc aatgaacgaa
     1261 gcggtgctcg tcttgccgtt ggtgcccgtc acaccgacgc tggacgccgc gcccacgaag
     1321 tcctcaatcc gtgcaagccg cgaaggcggc gtcacaagtc gcagtgctct tcgcgatgca
     1381 gtctcgatac gagtagtagg tcaagtcgag gatcatcccc gtcccgtaga tatcgagcgt
     1441 ggccttgcag gtctcgagcg gaagcgccgg acactccgcg gtcagcgccg cgcacacgct
     1501 gctcacgttc agggcctggt cgggaagacc gcggcacttg ccgtaggtgt acgcgcactg
     1561 ggtgctatcg gaggccctcg tgcagtctcg tactttcgtc accgtctcgg gcgacaacgg
     1621 gctcaaatct gcgctgcagc tttcgacgcc gatcgcgtcg cagcgctcgt gaatctccgt
     1681 gcaagcagcg acggcttcgc tcgcgagaca agtgctttcg cgcatcgatc gctcgcaagc
     1741 agcggcccgc gccgacgccg tcgccgagca caggtccgcc gtgctcagct tgaccaagca
     1801 acgcaccagc acgtcgaccg agccctgtcg ctctgccgcg tagcgctcgc acacttcact
     1861 cgcgggcacc ggaaccggga gcccttcgcc gctgggattc ggacagcgcg cttctgcgaa
     1921 cgacaacgtg ctgcaatcga acccgagacc ctcctcggcg ggcgcaccgc tgacacaggc
     1981 aacggccccg ccctcaccgc ccgctccccc gatcactccg cctgctccgc ccgtgccctc
     2041 catcgctggc gccccgccgg cgccgccctc cgtctgcccg cccgctcccg cagacggagc
     2101 gccgccttcc gagctcggtg caccggcgtc gacgccaccc gtacctgcat gagcgcctcc
     2161 caggccgcct tgcgattgcc cggaagcccc tgcagcgcca cctttcgcgc cgtcgaccgt
     2221 ctcgatcgtg caagccgacg cgatgctcgc gctgcccgcc agtacggcgg tgagcttcag
     2281 gaacttgtcg aaggaaatct gcattaggag ccacccattc tggggcaaag tgccccctga
     2341 gccgacgcac ggtagcgcgt tctacgcttt ctgtgcgagc gcttgcgcga tcgcctcgcg
     2401 gaggcggtaa tcgctcgggc tgcgaaccga agtctggctg gtattcgagg cgtacgagcc
     2461 gcgctctgtc ccagagctcg tggcgcccgc ggagtcgcct tcggggtgag ggtgaagtaa
     2521 cctcgcccct tcgacggtca cgcgtgcgct gcggaccgaa ggagaagaag aatgctgcga
     2581 gtcggttggt tgttcgcgtc cgtgctgagt tcgtgcgtcg tcggctgtgg aggcggtaac
     2641 ggcgcgagcg acgctcagct cgagaagggc cgcgacatgg cggcgacggg cgcgctgctc
     2701 tacgagcgca actgccaatc gtgccacggt gagcgcgggc agggcacgag caacgggcct
     2761 gcgatcttca acaagaagaa gctcgggaag cgcttcaaga acgctcaacg gctcttcgat
     2821 tacttggcga aggaaatgcc caaggacaat ccaggatcgc tcgacatcgc ccagtactgg
     2881 aatatcgaga ctttcatggt cgtcgcgagc ggtcgcaagc tcgaagatcg cttgtccgag
     2941 tcgaacgccg aagacacgaa gctcgacaag taaccaacat ggatcgaacc gccgtattca
     3001 tcgacgcggg gtacctgttc gcttcgggcg cccgtctgct ctccaacgcg cgagcgagcc
     3061 gagaagagct ctccctcgac aacgaggcct tggtcgcgac gttcacgcgt ttggctcgag
     3121 agctcactgg gctaccgatg cttcgcatct attggtacga cgcgagctac ggcggcccga
     3181 cga
//
LOCUS       DASTKS010001247         4281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_870522, whole genome shotgun sequence.
ACCESSION   DASTKS010001247 DASTKS010000000
VERSION     DASTKS010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870522"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1229
                     /locus_tag="VFQ35_26670"
     CDS             117..1229
                     /locus_tag="VFQ35_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9934326.1"
                     /translation="MARSGSGRPGVYLDWNATAPLRPEVIQAMVAAAELAWANPASTH
                     ALGRRARVEVEELREALGAALAVHPRDVVLTSGGTEANNLRLARAPGLVTSRLEHPSI
                     VRVAESLEAEGKPVRWISVPNSGRVEPDAVAELCGGMPAGSWVAVQAANHETGVLQPI
                     GAIAPRVHSAGLLLHVDAVQAFGKVDLSELQGADSIAVASHKIRGPKGIGALAFRRAV
                     PPPLLVGGSQERGLRPGTVDPVAAAGFRTAVMLLAPQRWAELAQSRDAFEGRLERVSD
                     RNGEGARLPHVLNRSFRGWKGDELVAALDLLGVCVSSGSACAAGTAEASPVITAMLGR
                     ERALSAVRISFGDATTDAELESARRAFETALGVNLE"
     gene            complement(1421..2218)
                     /locus_tag="VFQ35_26675"
     CDS             complement(1421..2218)
                     /locus_tag="VFQ35_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9934327.1"
                     /translation="MNPSRPDPRRRPAEPAPLPQQRGPRLVLGLQPVRAAIRAHGARL
                     GGVVLESADNPRLAAVERYAKDQGVEVVEVVGRASLDRLAGGVLHQGVGAWAPDLANS
                     EFGELLTDAQLLGVVLDRIQDPQNFGAVLRSSVGFCAGAVVWGEHSSAPLGPATLRAA
                     AGAAEFARLCRVASLPRALAEAADAGVQVIGLDAQADRALAAWDLRGPTLLVIGSEQS
                     GMTPAVRKACTGLARLVAPGPIESLNASVAAAIALYEATRQRAISDR"
     gene            complement(2199..2945)
                     /gene="pyrF"
                     /locus_tag="VFQ35_26680"
     CDS             complement(2199..2945)
                     /gene="pyrF"
                     /locus_tag="VFQ35_26680"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9934328.1"
                     /translation="MPVSEALGTARERLAFALDYPNLDAARRGAELVSESVGVLKVGL
                     ELFVRSGPAAVHLGHELCCDVFLDLKLHDIPETVERAVAAACEHGARYLTLHASGGPS
                     MLEAAARRAERENTGLVLLAVTVLTSLDASDLTAIGFQRPPAEQALMLARLAQSAGVR
                     GAVCSVAEVAALRAQLGKDAVLVTPGIRPRDGNASDDQKRVGTPGDAIRSGSSILVVG
                     RPIRDATDPKLAAARVLAEISEASGESVTS"
     gene            complement(2936..3709)
                     /locus_tag="VFQ35_26685"
     CDS             complement(2936..3709)
                     /locus_tag="VFQ35_26685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934329.1"
                     /translation="MAEQKESSVLFSLKELMNLEENRIKEEEAEKERRARQEADARAA
                     AERAAREAQERALREEEERRRAEEQRVREEAARLEAIRQGEIEKAKAEAEHRARMEAL
                     AAQQSHEQQLAALHRDQSKKRLKLIVAIGGATAVLALVGVGYAYSQAQERHQKEQQAM
                     LAQQQAASAELARLKADFEAASKKEEELKQSLANAKDEATRAKIESELANAKRDTETA
                     QRRVSGGGAARPSGGASKPSGGASKPCNCPPGDPLCSCL"
     gene            4118..>4281
                     /locus_tag="VFQ35_26690"
     CDS             4118..>4281
                     /locus_tag="VFQ35_26690"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HET9934330.1"
                     /translation="MAVDVCAHITGTVWKIEAKVGDSVSEGQTLVILESMKMEMPIES
                     PESGVVAEVAV"
BASE COUNT          634 a         1414 c         1485 g          748 t
ORIGIN      
        1 gaggaggaga tcgtcggcga gcgcttcaac ggcgctgctt tctacttcgt cgtgtccgag
       61 ttctcgatgg gtccggtcag cgagctgcca tcgcggccct tttcttaggc acgctcttgg
      121 ctcgcagcgg ctccggcagg cccggcgttt acctcgattg gaatgccaca gcgccgcttc
      181 ggcccgaggt gattcaggcg atggtggctg cggccgagct tgcctgggca aacccggcga
      241 gcactcatgc gctgggccgt cgcgctcgcg tcgaggtcga ggagctgcgc gaggcgctcg
      301 gcgctgcgct cgccgtgcat ccacgagacg tcgtgctcac gtcgggcggc accgaggcga
      361 acaaccttcg tttggctcga gcgcccgggc tcgtcacgag tcggctcgaa cacccttcga
      421 tcgtgcgcgt tgccgagtcg ctcgaggctg agggaaagcc cgtccgttgg atttctgttc
      481 cgaatagcgg ccgcgtcgag cccgacgccg tggcagagct atgcggcggg atgccggcgg
      541 gaagctgggt ggcggtgcag gcagcgaacc atgaaaccgg cgtgctccag ccgatcggcg
      601 cgattgctcc gcgggtgcac tcggccggcc tgctcctgca cgtcgacgcg gttcaagcct
      661 tcggtaaggt cgatttgagc gagctccagg gcgccgacag catcgccgtc gcctcccaca
      721 agattcgcgg cccaaagggg atcggcgcgc tcgcttttcg gcgcgcggtg ccgccgccgc
      781 tgctcgtcgg tggctcgcag gagcgcgggc tgcggcccgg gacggtcgat ccggtcgccg
      841 ccgctgggtt tcgaaccgcc gtcatgctgc tcgccccgca gcgttgggcg gagctcgcgc
      901 aatcgcgaga cgccttcgag gggcggctcg agcgggtgag cgaccggaac ggcgaaggcg
      961 ctcggctgcc tcacgtcctc aatcggtcgt ttcgcgggtg gaaaggcgac gagctcgtgg
     1021 cggcgctgga tctcctcggc gtttgcgtct cgagtggtag cgcgtgtgcc gccggcaccg
     1081 ccgaagcatc gcccgtaatt acggccatgt tgggccgcga gcgggccctc tccgcggttc
     1141 gcatcagctt cggcgacgcc acgaccgacg cagagctcga gtccgctcga cgcgcgttcg
     1201 agaccgccct cggcgtgaac ctcgagtagc acctgatcct ctcgcgcgtt cgctgctctg
     1261 tgaccttagc tccgtgcgct aagtgctcgg gaacgcgtgt cttcgaggaa gggcagtctc
     1321 ggcgaggggg cgatccactg aaaggactcc cgggcgttcg gttcgggacg ggtgttcagg
     1381 tggacgatgt aagcttcatg taaaacatga ggtttgcatc ttatctatct gaaattgcac
     1441 gctgtctcgt tgcttcgtac agagctatcg ccgctgccac cgaagcattg agcgactcga
     1501 tcggccctgg ggcgacgagg cgagcgaggc cggtgcaggc cttgcgcacg gcgggagtca
     1561 ttcccgattg ttcgctgccg atgacgagca gggtcgggcc gcgcagatcc cacgcggcaa
     1621 gggctcgatc ggcttgggcg tcgagcccaa tcacttgcac gcccgcgtcg gccgcttcgg
     1681 ccagagcgcg cggcaatgac gctactcgac acaagcgcgc gaactcggcg gcaccagcgg
     1741 cggcacgcag ggttgccggc ccgagcggtg ccgaagagtg ttcgccccac acgaccgcgc
     1801 cggcgcaaaa gcccacgcta ctgcgcagca cggccccgaa gttctgtggg tcctggattc
     1861 ggtccagcac gaccccaagt agttgggcat cggtgaggag ctcgccgaac tcgctattcg
     1921 cgagatccgg cgcccacgcg ccaactccct ggtgaagcac accgcccgcg agacggtcga
     1981 gcgaggcgcg cccgaccacc tcgaccactt cgacgccctg gtctttcgca tagcgctcga
     2041 cggccgcgag gcgcgggttg tcggcgcttt cgaggacgac gccgcccaag cgcgctccgt
     2101 gcgcgcggat ggcggcgcgc accggctgca acccgaggac gagacgcggg ccgcgttgct
     2161 gaggaagggg cgccggctcg gccgggcgac gccgcggatc aggacgtgac ggattcaccg
     2221 ctcgcctccg aaatctcggc gagaacgcgg gcggcggcaa gtttcgggtc ggtcgcgtcg
     2281 cgaatcgggc gccccacgac caggatgctc gagccgctgc gaatggcgtc gccgggcgtg
     2341 ccgacgcgct tctggtcgtc gctagcgttg ccgtctcgcg ggcgaatccc cggtgtgacg
     2401 agcacggcgt ctttgcccag ctgggccctc aaggccgcga cctcggcgac cgagcacacg
     2461 gcgccacgta cgcccgcgct ttgggcgagc cgggcgagca tcaacgcttg ctcggcgggc
     2521 ggccgctgaa agccgatcgc ggtgaggtcc gaagcgtcga gtgaggtgag cacggtgacc
     2581 gccaagagca cgagccccgt attttcacgc tcggcccgcc gcgccgcagc ctccaacatc
     2641 gagggcccac cgctcgcgtg gagcgtcagg taacgcgcgc cgtgttcgca ggctgcggcc
     2701 acggcgcgct ccaccgtctc cggaatatcg tggagcttga gatccaagaa gacgtcgcag
     2761 caaagctcgt ggccgaggtg cacggcggcg gggcccgagc gcacgaacag ctcgagcccg
     2821 actttcagta cgccgaccga ttcgctcacc aactccgcgc cgcgtcgggc ggcgtcgagg
     2881 ttgggatagt cgagcgcaaa agcgaggcgc tctcgagcgg ttccgagcgc ttcgctcaca
     2941 ggcacgagca cagcggatcg ccaggcgggc agttacacgg cttgctcgcg ccgccgctcg
     3001 gcttgctcgc gccgccgctc ggacgggcag cgccaccacc ggaaacgcgg cgctgggcgg
     3061 tctccgtgtc cctcttggcg ttcgcgagct ctgattcgat cttggcgcgc gtcgcctcgt
     3121 ctttggcgtt ggcgaggctc tgcttcagct cctcttcctt cttggaagcg gcttcgaagt
     3181 cagccttgag gcgagcaagc tcggcggaag cagcctgctg ctgcgcgagc atcgcctgtt
     3241 gctccttctg gtggcgctct tgcgcttggg agtaggcgta acccacgccg acgagcgcca
     3301 agacggccgt ggcgccgccg atcgcgacga tgagcttcag ccgcttcttc gactgatctc
     3361 ggtggagcgc tgctagctgt tgctcgtgcg actgctgcgc cgcgagcgcc tccatgcgag
     3421 cgcggtgctc ggcctcggcc ttcgcctttt cgatttcgcc ctggcgaatg gcctcgagac
     3481 gcgcggcttc ctcacggacc cgctgctcct cggcgcggcg ccgctcttcc tcttcacgca
     3541 acgcgcgctc ttgcgcttca cgcgccgcac gctctgccgc cgcccgcgca tccgcctcct
     3601 gccgagcgcg gcgttctttc tccgcctctt cctctttgat cctgttctcc tcgaggttca
     3661 tcaactcttt gagtgagaag agaaccgagc tttccttctg ttcggccatg ggggagtctt
     3721 cctcgatcgg ggcgacgggc ttcagggttt acgacccgcc tcacgcgggt agcatccggt
     3781 cattgtggga gtcaagcagg tggggagcac ctatgtagtc ggggcgcgca cgctcgcgac
     3841 gcacgcgcgc gacagcgaac cgtactcgat gttttggcgc gtgcccagca tggacacggc
     3901 attctccgct gcgcttgggt cgaagttttc cgatagagcg aggacgcttt ccgtacgtcg
     3961 gcccggaggc tccgagaggc gcgtccgaga aatgctcctg agccgttacg gtccgagcga
     4021 cgggcggccg gagggcgtgc gcgtcgtgcg gtgaaaagcg atgcgcgcgt gtttcggcga
     4081 gacggacgcg gcgagggaag ctgtatgctc gagggcgatg gcagtcgacg tgtgtgcaca
     4141 catcaccgga acagtctgga agatcgaagc gaaagtcggc gattcggtga gcgagggtca
     4201 gactctggtc atcctcgaat cgatgaaaat ggagatgccg attgagtcgc ccgagtcggg
     4261 cgtcgtggcg gaagtagccg t
//
LOCUS       DASTKS010001248         5778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_423430, whole genome shotgun sequence.
ACCESSION   DASTKS010001248 DASTKS010000000
VERSION     DASTKS010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423430"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFQ35_26695"
     CDS             complement(<1..492)
                     /locus_tag="VFQ35_26695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9934331.1"
                     /translation="MTSIEVHEGDLLWSPTVERVERARIRAYMAWLESTGVAVCHDFA
                     ALHAFSVERPGAFWRSIADYFEVRFRSRAQRELDGAMPNARWFEGATLNYAEHAFARR
                     DGQVALVFRSESGERASLTYAELAERVARARAGMRRLGVGRGDRVAALLPNRIEAVVA
                     FLAA"
     gene            complement(489..1622)
                     /locus_tag="VFQ35_26700"
     CDS             complement(489..1622)
                     /locus_tag="VFQ35_26700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934332.1"
                     /translation="MPSAGEVGPWLFGARIDLLLFAGASVASLAIGGLGRAMGLSTLP
                     EGAWLGLVLAVDVAHVHATWFRTYLDRAELARKPLRYALVPLIAYAVAALAYRQGPLV
                     FWRGLAYLAVFHFVRQAVGWVALYRAKAGPYRWFDRALDDAAVYAATLYPLLVWHVHA
                     AEKGFSWFVGGDFVTLPLASWLPAARLVWAGSLIAFFAREAARWHSTRTVMLGKSLVV
                     ACTALTWHVGIVVNDSDFMFTASNVIPHGVPYAWLLFAYTRRRAKEAPAFRFAQVALG
                     GFAAFCAVLVGFAFIEQLAWDRLVEHERPWLFGDASPLPAPWLAWVVPLLALPQATHY
                     LLDGLLWRRADSRELPAQRALLRADRAEPTSELPPLGLEVAAK"
     gene            complement(1682..3289)
                     /locus_tag="VFQ35_26705"
     CDS             complement(1682..3289)
                     /locus_tag="VFQ35_26705"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9934333.1"
                     /translation="MTLSRRELLCGLLGVPLGFEACRRSPRTVPGTLRGARIDLGHRL
                     LEATRERVSAPPEQVPVVIVGAGPSGLSAAWRLSRLGIEKYVVLDLERLAGGTSASER
                     DGVVPYPWGAHYVPLPRADNAELVRLLEEVGAVRVDPSGAPEGLEQVRVRAPEERLFI
                     DGTWIEGLFPSSRATESDRAELLRFEAEIQRYAAFRDGGGRRAFALPMRRCSDASEIT
                     ALDKVSAGQWLRQNGFRSKLLHWYVEYACRDDYGASLERTSAWAMFFYFCARVGPSGD
                     TAPFLTWPEGNGRVARHLAERVGPRLRLNRLVTDVAPSADGVDVVALDEDGRAETYRA
                     EHVILAVPKLVVRRILRPSPGAPQKLEGFHYAAWLVANLHLSKRPRSRGFPFAWDNVL
                     FDSVALGYVVATHQTLADSGPSVWTYYRPFWEHAEPEARKILRSLDHATAAEAVLADL
                     SRAHENLESCVERLDVYRWGHAMVTPLPGFIWGEERRSAAAPQGRVVFAHSDLSGLPL
                     LEEAFDRGVSAGEEVARQRGLTFDASG"
     gene            3352..4470
                     /gene="trpS"
                     /locus_tag="VFQ35_26710"
     CDS             3352..4470
                     /gene="trpS"
                     /locus_tag="VFQ35_26710"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9934334.1"
                     /translation="MTDDTGRMSDAEDEKPTTTFEEAQRRSQALLADLPKNPGRYRVL
                     TGDRPTGPLHIGHYFGSLKNRVDLQNLGVETFVVIADYQVLTDRDTVDAVADNVRGLV
                     LDYLSIGLDPEKSRTHIFCHSHVPALNQLLVPFLTLVGMGELERNPTVKEEISAAGLT
                     HVNAAMYTYPVHQAADILFCKGNVIPVGKDQLPHIELTRKIARRFNTRFKAKPSVFRE
                     PDALLSAGTKILGLDGNQKMSKSRGNAIMLRATADETRDLVKRAKTDTERRITYDPEA
                     RPEVANLLLLISLCTGEAPAEIADKLGDAGGGGLKKLLTDTLNETLRPIRERRAELAR
                     DTESVVKSVLTRGTAEANRVAEATLAEMRRAMNMDYGL"
     gene            4741..>5778
                     /locus_tag="VFQ35_26715"
     CDS             4741..>5778
                     /locus_tag="VFQ35_26715"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9934335.1"
                     /translation="MENPLLSLSRPLAFGSIRAEHVVPAIQKLLADAEQRLAAMSPDG
                     SSYAATLGELEAATEQLELVMGVVEHLEGVSTTTELREAYNAVLPEVTALWTSIPLRE
                     NVWQALRAFATTSEAESLDPTRKRLLEKKLAEFRREGATLEPAQKQRLSEIDRELGLI
                     TNKFSQNVLDATNAYELLLTDAARLDGLPASALEAAAESAKAKGKSGYRLNLQAPVVT
                     AVLTYANDASLREEIWRAYNGRAASGEFDNRGLIGDILRLRRERALLLGFKDFADLVT
                     EERMAKDGEHAQRFIDELTEKTKDAFVRENEDLLAFRKKLEGPNAPPLRPWDVSYYTE
                     KLRRERYDLDEE"
BASE COUNT         1121 a         1993 c         1844 g          820 t
ORIGIN      
        1 ggcggcaagg aaggcgacca ccgcctcgat tcgattcggt agcagcgccg cgacccgatc
       61 gccgcgtccc acaccgagtc ggcgcatccc ggcacgggca cgcgccacgc gctcggcgag
      121 ttcggcgtag gtcagcgagg cgcgctcgcc gctctcggag cggaacacga gcgccacctg
      181 cccgtctctg cgagcgaagg cgtgctccgc gtagttcaac gtcgcgcctt cgaaccagcg
      241 cgcattcggc atcgcgccgt cgagctcacg ctgcgcgcgc gagcgaaacc gcacctcgaa
      301 gtagtcagcg atgcttcgcc aaaacgcgcc cgggcgttcg acgctgaacg cgtggagagc
      361 ggcgaaatcg tggcacaccg cgacgccggt gctctccaac cacgccatgt acgcgcggat
      421 ccgcgcccgc tctacgcgct cgaccgtcgg cgaccacagc aggtcgcctt cgtgcacttc
      481 gatgctcgtc atttggcggc tacctccagg ccgagcggcg ggagctcgct cgtaggttcg
      541 gctcggtcgg cgcggagcag cgctcgctgc gccgggagtt ctcgactatc ggccctgcgc
      601 cacaagagtc cgtcgagcag atagtgcgta gcttgcggca gcgcgaggag cggcaccacc
      661 cacgcgagcc acggcgccgg gagagggcta gcgtcgccga acagccaagg ccgctcgtgc
      721 tccacgaggc gatcccaggc gagctgttcg atgaaggcaa aaccgaccaa cacggcgcag
      781 aacgctgcga acccgccgag cgcgacttgc gcgaagcgaa aggcgggcgc ttccttcgcg
      841 cggcggcgag tgtaggcaaa gagtagccaa gcgtacggaa cgccatgagg gatcacgtta
      901 ctagccgtga acatgaagtc gctgtcgttc acgacgatcc cgacgtgcca ggtgagagcc
      961 gtgcaagcga cgacgagcga cttcccgagc atgacggtgc gcgtcgaatg ccaacgagcc
     1021 gcctcccgcg cgaagaaggc tatcagtgag ccggcccaaa cgaggcgcgc ggccggcaac
     1081 caggacgcga gcggcagcgt gacgaagtct cccccgacga accaagaaaa ccccttttct
     1141 gctgcgtgaa cgtgccagac gagcagcgga tacaacgtcg ccgcgtaaac cgccgcgtcg
     1201 tcgagggctc tatcgaacca gcgataaggc cccgccttgg cccgataaag agcgacccag
     1261 cccaccgcct gccgcacgaa atggaacacg gcgaggtacg cgagcccgcg ccagaacacg
     1321 agcggcccct gccggtacgc gagcgcagcg acggcatacg cgatgagcgg gacgagcgcg
     1381 tagcgtagcg gcttgcgagc gagttcggcg cggtcgaggt aggtacgaaa ccaagtcgcg
     1441 tgcacgtgcg ccacgtcgac ggcgagcacg agcccgagcc acgcaccctc gggcaacgtg
     1501 gagagcccca tcgcgcgacc gagcccgccg attgcgagcg acgcgaccga agcgccggcg
     1561 aacaacagca gatcgatgcg cgcaccgaag agccatggcc ctacctcgcc agcggacggc
     1621 acaagccgcg agccctgcac gcaacgatgg tgaaccggct cgccgtttac ggcaagccac
     1681 ctcagccgga ggcgtcgaag gtcagcccgc gctgccgcgc cacctcttcg ccggccgaaa
     1741 cgccgcggtc gaatgcctct tccaaaagcg gtaacccgga aaggtccgaa tgggcgaata
     1801 ccactcgccc ttgaggagcc gccgcgctcc gccgttcctc tccccagatg aagcccggaa
     1861 gcggcgtgac catcgcgtgc ccccagcggt acacgtccaa gcgttcgacg cagctctcga
     1921 gattttcgtg ggcgcgagag aggtcggcga gcaccgcttc ggccgccgtt gcgtgatcga
     1981 ggcttcgcaa gattttacgc gcctcgggct cggcatgctc ccaaaacggg cggtagtacg
     2041 tccacacact cgggcccgag tcggccaagg tctggtgcgt cgcgacgacg tagccgagcg
     2101 ccacgctatc gaagagtaca ttgtcccacg cgaacgggaa gccgcgcgac cgcgggcgct
     2161 tcgaaaggtg caggttggcg acgagccacg ccgcatagtg aaagccctcg agcttttggg
     2221 gtgcacccgg ggatggtcgc aggatgcggc gtacgacgag ctttggaacc gccaagatga
     2281 cgtgttcggc gcgatacgtc tcggcgcgcc catcttcgtc caacgccacc acgtcgacgc
     2341 cgtccgcgct cggtgcaacg tcggtgacca gccgattcag tcgcaagcgg gggcccacgc
     2401 gctcggcgag gtgccgcgcc acgcggccgt ttccttccgg ccaagtcaag aacggcgccg
     2461 tgtcaccgct cggcccgacc cgagcgcaaa agtagaagaa catggcccag gcgctcgttc
     2521 gctcgagcga cgccccgtag tcgtctcggc aagcgtactc gacgtaccag tgcaagagct
     2581 tcgatcggaa accgttctgg cgcagccact gcccggccga gaccttgtcg agagcggtga
     2641 tttcgctcgc atcggagcat cgacgcatcg gcagtgcgaa ggcgcgtcgg cccccgccgt
     2701 cgcggaacgc ggcgtatcgc tgaatctcgg cttcgaagcg aaggagctcg gcgcgatccg
     2761 actcggtcgc gcgcgacgat gggaacaagc cttcgatcca cgtaccgtcg atgaacagcc
     2821 gctcttcggg ggcccggact cgcacttgct cgagcccctc gggcgcacca ctcgggtcga
     2881 cccgcaccgc gccgacttcc tcgagcagcc gaacgagctc tgcattgtcg gcgcgcggga
     2941 gcggaacgta gtgcgctccc cacggatacg ggacgacgcc gtcgcgctcg ctcgcgctgg
     3001 tcccacccgc taggcgctcc aagtcgagca ccacgtactt ctcgatgccg agccgtgaca
     3061 gccgccaagc cgccgaaagc ccgctcggcc ctgcccccac gatcacgact ggaacctgct
     3121 cgggaggcgc gctcacccgc tcgcgagtgg cctcgagcaa gcgatgcccc aagtcgatgc
     3181 gggcgccgcg caaagtcccg ggcaccgtgc gcggcgaccg gcggcacgcc tcgaaaccga
     3241 gcggcacgcc gagcaggcca cacaagagct cgcgacgaga aagcgtcacc aatccgggca
     3301 gtaccgcatt ttgcgtccgc cgtggtgccc ccgcttgcga gcgcgctcga aatgaccgat
     3361 gacacgggcc ggatgtccga cgccgaagac gagaagccca cgaccacctt cgaagaagcc
     3421 cagcgccgtt ctcaagcgct gctcgctgat ctaccgaaga atcccggccg ctatcgcgtg
     3481 ctgaccggcg atcggccgac ggggccgctg cacatcggcc actacttcgg ctcactcaag
     3541 aaccgcgtgg acctgcagaa cttgggtgtc gagaccttcg tcgtgatcgc cgactaccag
     3601 gtgctcacgg accgcgacac ggtcgacgcc gtcgcggaca acgtccgcgg cttggtgctc
     3661 gactacttgt cgattgggct cgatcccgaa aagagccgca cgcacatctt ttgccacagc
     3721 cacgtcccgg ccctgaacca gctgttggtg ccgttcctca ccttggtcgg catgggcgaa
     3781 ctcgagcgca atccgaccgt caaagaagag atcagtgcgg cgggtctcac tcacgtgaac
     3841 gccgcgatgt acacgtaccc ggtgcatcaa gcggcggaca tcctcttctg caaaggcaac
     3901 gtcatccccg tcgggaaaga ccaactgccg cacatcgagc tcacccgcaa aattgctcgg
     3961 cgcttcaaca cgcgcttcaa agcgaagccc tccgtattcc gtgaaccgga cgcgctcctc
     4021 tctgcgggca ccaagatctt gggcctcgac ggcaaccaga agatgagcaa gagccgcggc
     4081 aacgccatca tgctgcgagc gacggcggac gagacgcgcg acctcgtgaa gcgcgccaaa
     4141 accgacaccg agcgtcgcat cacctacgat cccgaggcgc gtcccgaggt cgcgaacttg
     4201 ctcctgctca tttcgctctg taccggcgaa gcgcccgctg aaatcgcgga caagctcggg
     4261 gatgccggcg ggggcgggct gaaaaagctc ctcaccgaca cgctcaacga gacgcttcgc
     4321 cccatccgag agcgccgcgc cgagctcgcg cgcgacacgg agtcggtcgt gaagagcgtg
     4381 ctcacgcgcg gcaccgcaga ggccaatcgc gtcgccgaag cgacgctcgc cgagatgcgc
     4441 cgggccatga acatggacta cgggctctaa ggcgcgaaaa cggttaggaa gtcgtccaag
     4501 gttgcggaaa acggctccgc acagggtatc cgcgccccga gcactcagag ttctctcgta
     4561 caatgtgttc gcggcatcgg ctcgggattt caggcagacc gacgagcttt ggcgcgccgt
     4621 tttcggcggg ttcgcggcct ctttgcccga tccgaaccgc gcgagcgcgt cgttttggca
     4681 agcctgccgc tcttgcggtt ggaccaaccg gtacctaccg agtaggagta ggctagagtc
     4741 atggaaaacc cgctgctttc cctctcccgt ccactcgcct tcgggagcat tcgcgccgag
     4801 cacgtcgtcc ccgctatcca gaagctgctc gcggatgccg agcagcggct cgccgcgatg
     4861 tcgccggacg gctcgagcta tgcggcgacg ctcggtgagt tggaggctgc aaccgagcag
     4921 ctcgaactcg tcatgggcgt ggtggagcac ctcgaaggcg tgagcacgac gaccgagctc
     4981 cgcgaagcgt ataacgccgt gttgcccgag gtgaccgcgt tgtggacgtc gattcccctg
     5041 cgcgaaaacg tctggcaagc gcttcgcgcc ttcgcgacca cgagcgaggc cgagtcgctc
     5101 gacccaaccc gtaagcggtt gctcgaaaag aagctcgccg agttccggcg cgagggcgct
     5161 acgctcgaac ctgcccaaaa gcaacgactc agcgagatcg accgcgagct cggcctcatc
     5221 accaacaagt tctcgcagaa cgtgctcgac gcgaccaacg cctacgagct cttgctgacg
     5281 gatgcggctc gcctcgacgg gctaccggcg tccgcactcg aagccgccgc cgagagcgcc
     5341 aaagccaagg gcaagagcgg ctatcggctg aacttgcaag ccccggtggt gaccgcggtg
     5401 ctgacgtacg cgaacgacgc gagcttgcga gaagaaatct ggcgcgccta caacggacgc
     5461 gcggcgagcg gggagttcga caatcgcggg ctcatcggcg acattttgag gctgcggcgc
     5521 gagcgcgcgc tcctgctcgg gttcaaggac ttcgccgatc tcgtcaccga ggagcgcatg
     5581 gcgaaggacg gtgagcacgc ccagcgcttc atcgacgagc tgaccgaaaa gaccaaagac
     5641 gccttcgttc gcgagaacga ggacctgctc gcattccgca agaagctcga aggcccgaac
     5701 gcgccaccgc tacgtccctg ggacgtctcg tattacaccg aaaagctgcg tcgcgaacgc
     5761 tacgacctcg acgaggag
//
LOCUS       DASTKS010001249         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1094972, whole genome shotgun sequence.
ACCESSION   DASTKS010001249 DASTKS010000000
VERSION     DASTKS010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094972"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFQ35_26720"
     CDS             complement(<1..325)
                     /locus_tag="VFQ35_26720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9934336.1"
                     /translation="MTSAPELAEKIALTHELERVREENAALRRELSTATHESEENTRF
                     RELAEAMPQLVWTCTADGACDYLGPQWVEYTGTPESEQLGSAWLEKLHPDDRERVNRE
                     WTLAAS"
     gene            complement(318..2345)
                     /locus_tag="VFQ35_26725"
     CDS             complement(318..2345)
                     /locus_tag="VFQ35_26725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934337.1"
                     /translation="MRPIDILLIEKRSAGSTTLDTLGRQRLLNSVHTVNGHPEALGYL
                     RQQGKFAHAPRPDLILLDVESCDESEIEALSAFHFDSPTKTVPLVLLTSAECAPPERN
                     ACGALHWITKPLDLAKLTQALTGLEKYWFEVLTVPDAPATQQPRHDEAPPSTPTSLET
                     LRLLLIEDSPSDALLFRHAVDNNPFFKVNLTHVARLRDVDPVLKTAPFDLIVTDLCLP
                     DASGLETFRQVQARASGVPVIVLTGTENDVAGLETLRHGAQDYIVKGELSSGAIARAA
                     RYAVDKKRYQERLQQSRRLEAVGRLTAGVAHDFNNILAVIRGNAELLADEGGSVAESA
                     ADICSAADRGVALARQLLSFSRQRAMSPTVIDLNDLLGKFTKTLLRLLGDKVDVELEF
                     SETLPPVSGDAGMLEQVVSNLALNARDAMPEGGQLKLRTTAVEFDADSIRSHPAGTPG
                     RFVELAVTDTGSGMTPDVLERAFDPFFTTKPFGAGSGLGLATVHGIIQQHRGWLEVES
                     SVGVGTTFRIFLPATEGSVQQSQPPSMRRPGGGETILLVEDESSLRALVAKRLSERGY
                     RVLEAGDSGEALRQWSERGHEIDLVFCDITLPAGANGRELVEGFLVQRPNVPVVLTSG
                     YSPDLTNGAFPLEEGVNFLQKPYELGKLLGTVRRRLDARATRDERGAAAND"
BASE COUNT          415 a          811 c          797 g          470 t
ORIGIN      
        1 cgctcgcggc gagcgtccat tcgcggttca cgcgctcgcg atcgtctgga tgaagcttct
       61 cgagccaggc gcttccgagt tgctcgctct cgggggtgcc ggtgtattcg acccattgag
      121 ggccgaggta gtcgcatgcg ccgtcggccg tacacgtcca aacgagctga ggcatcgcct
      181 cggccagctc gcgaaagcgc gtgttttctt cgctctcgtg tgtcgcggtg gacagctcgc
      241 gccgcagcgc cgcgttttct tcccgaacgc gctcgagctc gtgcgtgagc gcgatctttt
      301 cggcgagttc gggcgcgcta gtcattggcg gccgctcctc gctcgtctcg cgtcgctcgc
      361 gcatccagcc ggcgtcgcac cgttcccaac aatttcccga gctcgtaggg cttttgcaag
      421 aagttgacgc cctcttcgag ggggaacgcg ccgttcgtca aatcggggct gtagccactc
      481 gtgagcacga ccggaacgtt cgggcgctga actaaaaagc cctccaccag ttctcgcccg
      541 ttcgctcccg ctggcaaggt gatatcgcag aacacgaggt ctatttcgtg gccgcgttcc
      601 gaccactgtc gcaacgcctc gcccgaatca ccagcctcga gaactcggta gccgcgctct
      661 gacaggcgct tcgcgaccag ggcgcgcagc gacgactcgt cctcgacgag gaggatcgtc
      721 tcgccgccgc cgggtctgcg catcgaaggt ggctgactct gttgaacgct gccttcggtg
      781 gcgggcaaga agatcctgaa ggtcgtcccc acgccaacgc tgctttcgac ctccaaccac
      841 cctcgatgtt gctggatgat cccgtgcacg gtcgcgagcc cgagcccgct gccggctccg
      901 aaaggcttcg tcgtgaagaa cggatcgaag gcgcgctcga ggacgtccgg agtcatcccc
      961 gatccggtgt ccgtcacggc gagctcgacg aagcgcccgg gcgtccccgc cggatgggaa
     1021 cgaatcgagt ccgcgtcgaa ctccacggcc gtcgtgcgaa gcttcaactg gcctccctcg
     1081 ggcatggcgt cccgcgcgtt gagcgccagg ttcgatacga cttgctcgag cataccggcg
     1141 tcgccggaaa cgggcggcag ggtctcgctg aactcgagct cgacgtcgac tttgtcgccc
     1201 aagagccgca gcaacgtctt cgtgaacttg ccgaggaggt cgttgaggtc gatgacggtc
     1261 gggctcatcg cgcgttggcg actgaagctc aacagttgac gcgcgagcgc gactccgcgg
     1321 tccgcggccg agcaaatgtc ggctgcagac tctgcgaccg agcccccttc atcggccaat
     1381 agctctgcgt tgccccgaat gacggccagg atgttgttga agtcgtgggc cacgcccgcc
     1441 gtgagtcgcc caaccgcttc gaggcgccgc gattgctgca gacgttcttg atagcgcttc
     1501 ttgtccactg cgtagcgcgc ggcccgcgcg atcgcgccgc tgctcagctc gcccttgacg
     1561 atgtagtctt gcgctccgtg ccgcagcgtc tcgagcccgg ccacgtcgtt ctcggttccg
     1621 gtgagcacga tgaccggcac gccgctggcg cgcgcttgga cctgacgaaa cgtctccagc
     1681 ccgctcgcgt ctggcaagca aagatccgtg acgatcaagt cgaacggagc cgtcttcaga
     1741 accgggtcca cgtcgcgcag acgtgcaacg tgcgtgaggt tcactttgaa gaaggggttg
     1801 ttgtcaaccg cgtgccgaaa caagagtgca tcgctcgggc tgtcttcgat gaggagcaag
     1861 cgcagcgttt cgagcgaagt cggcgtggac ggcggagctt cgtcgtgcct tggctgctga
     1921 gttgccggtg catccggcac cgtcagcact tcgaaccaat acttctccaa gcccgtcagc
     1981 gcttgggtca gcttcgcgag gtcgagcggc ttcgtaatcc aatgcagcgc gccgcaagcg
     2041 ttgcgctcgg gcggtgcaca ttctgccgat gtcagcagga ccaaggggac tgtcttggtg
     2101 ggcgagtcga aatggaatgc gctcagcgcc tcgatctcgc tctcatcgca cgactcgaca
     2161 tcgagcagga tcagatctgg ccgcggcgcg tgcgcgaact tgccctgctg acgaagataa
     2221 cccagcgcct ccgggtgacc gtttaccgta tgcacggagt tgaggagacg ctgacggcct
     2281 agagtatcga gcgtcgtcga gccggcagag cgcttctcta tcagcaagat gtcgattggc
     2341 cgcatcaaat ccctcgaggg gcttcgtcgg gaacttcgag aaccagtcgc ctccgacttc
     2401 cctcgacgag cgggcgagcc agggcaaagc caagccgcgc ggtcatggga ccggagcgcc
     2461 gtcgtatcgc gtgggagcct tgggaggcag cct
//
LOCUS       DASTKS010001250         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_423597, whole genome shotgun sequence.
ACCESSION   DASTKS010001250 DASTKS010000000
VERSION     DASTKS010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423597"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFQ35_26730"
     CDS             <1..362
                     /locus_tag="VFQ35_26730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934338.1"
                     /translation="EESPRAFASSLLVLDFLLTGPTEIVLALDGESTSGEGLARALAS
                     RFLPSRVQTVVSASRPNPTRLARDKGPVEGRAAVYVCRDFACQRPVTEPAELLPMLDA
                     DQRQRTAREALRRDDAV"
     gene            487..1617
                     /locus_tag="VFQ35_26735"
     CDS             487..1617
                     /locus_tag="VFQ35_26735"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934339.1"
                     /translation="MLLPLIAGVALVVSTVWCVAGLIAVFRVTRSRPAPLPTEQLPAV
                     SVLKPLCGSDPNLLANLESFFEQDHGDFELLFGVVDEADPALAAVRAARARHPEIACR
                     VIVHSGHGASNPKVDNLLGLLPQAKHDLVLVSDSNVRAPRHYLRELAALYVEQRPGLV
                     TNLFSGSDEASLGGALESVELAGFCAPGVALPTLLGDPLLVGKSALFSRRRFEQLGGF
                     QRLRDVLAEDFVMGKTFAHAGDAIVIAPTVLANVVGKLSVRSALRRHLRWSMLRFRLR
                     PVAAALEPFTNPLALLPVAWYLLGPWALAWAAACWLFRDAGSWLVLRGAERVWLPLLL
                     SPLRDALSLGVWVVAPLKHHVSWRGKRFRLGAGTLLYSEQAR"
     gene            complement(1631..>2678)
                     /locus_tag="VFQ35_26740"
     CDS             complement(1631..>2678)
                     /locus_tag="VFQ35_26740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934340.1"
                     /translation="ELRTPMTVVIGATELLLETNLDAEQRAAVKGVHRSGQELLSIIN
                     QVLDEPRDSTRSIVPSSEAPEQSAEVGGYALVVEDNEFNQALITHTLSATGCHVDAVS
                     NGMDALNRFGSRDYDVVLMDCHMSGLDGFETTREIRRMERGKRRVPIIAVTAGGVAET
                     RRNCLAAGMDDYISKPFTLATLRARVAYWITRGRDSRPASAEVPPTVAPTHSTTGHLD
                     LSRLEELAFDEGSSDVAVLLTQIFLEDITKRVRALRDAAMSTDEAACLLVAHAIKGAC
                     GNFGAVGMAQLAEQAEKRCKAHALDEVQSIAHNLVDELSIVRELLDAHGLIPDARGVA
                     NPRLRRPSRVPPAV"
BASE COUNT          397 a          883 c          906 g          492 t
ORIGIN      
        1 tcgaagagtc tccgcgagcg ttcgcgagca gcttgctcgt gctcgacttc ttgctcaccg
       61 gccccaccga gatcgtcctg gctctcgacg gcgagtcgac gagcggcgaa gggctcgctc
      121 gggcgctggc aagccgcttc ttgccgagcc gcgtgcagac cgtggtgtcc gcgtcccgcc
      181 cgaacccaac gcgtctcgcg cgagacaaag gtccggtgga ggggcgtgcg gcggtgtacg
      241 tgtgtcgaga ctttgcttgc cagcgacccg tcaccgagcc cgccgagctt ttgcccatgc
      301 tcgacgccga tcagcgccaa cgaacggcgc gcgaagcgct gcgcagggat gacgcagtgt
      361 gaaaagcgct acgctcaact ggttctacca gttcacactt cggcctgcga gtcgtacgta
      421 acatattgaa attacggtcc tggactgaat gatgcagcgt ggcacgcggc tcgctagaca
      481 ggccgcatgc tgttacccct catcgccggc gtcgccctgg tggtttcgac ggtctggtgc
      541 gtggctgggc tcatcgccgt cttccgcgtc acgcggtcgc gcccggcgcc gctaccaact
      601 gagcagctgc cggccgtcag cgtgctcaag cccttgtgtg ggagcgaccc caatctactc
      661 gcgaacctgg agagcttctt cgagcaagac cacggtgact tcgagcttct gttcggcgtc
      721 gtcgacgaag cggacccggc gctcgcagcc gtgcgcgcgg cccgagcacg ccaccccgag
      781 atcgcctgca gagtgatcgt gcactcggga cacggtgcgt cgaatcccaa agtcgacaac
      841 ctcctcggtt tgctcccgca ggcgaagcac gacttggttt tggtgagcga cagcaacgtg
      901 cgcgctccgc gtcattacct gcgcgagctt gcggcgttgt acgtcgagca acgaccgggc
      961 ctcgtcacga acctcttctc tggaagcgac gaagcatcgc tcggaggagc gctggagagc
     1021 gtcgagctcg caggcttctg cgcgccgggc gtagcgttgc ccacgctgct cggagatccg
     1081 ctgttggtcg gcaaatcggc gcttttttcg cggcgaaggt tcgagcagct cggaggcttt
     1141 cagcggctcc gcgatgtctt ggcggaggac ttcgtgatgg gcaagacctt cgcgcacgcg
     1201 ggcgacgcga tcgtcatcgc gcccaccgtg ctcgccaacg tggtcggcaa gctcagcgtc
     1261 cggagcgctc tccgccgtca cttgcgctgg tcgatgttgc gcttccgttt gcgcccagtc
     1321 gcggcggcgc tggagccatt cacgaacccg ctcgcgctgt tgcccgtcgc ctggtaccta
     1381 ctcggtccgt gggccctcgc gtgggcggcc gcgtgctggc tattccgcga cgccggcagt
     1441 tggctcgtcc tgcgcggggc ggagcgcgtc tggctgccgc tcttgctgag cccactgcgc
     1501 gacgcgctct cgctcggggt gtgggtggtc gcgccgctca aacatcacgt ctcgtggcgt
     1561 ggcaagcgat tccgcctcgg cgccggaacg ctgctctact ccgagcaagc gcgctgacga
     1621 ccaatgcggc tcaaactgcg ggcggaacgc gcgaaggtcg gcgcaggcgc gggttagcga
     1681 cgccgcgcgc atccgggatc aacccgtgcg cgtcgagcaa ctcccggacg atggagagct
     1741 cgtcgaccag gttatgcgca atcgattgga cctcgtcgag ggcgtgcgcc ttgcaacgct
     1801 tttccgcctg ttcggcgagc tgagccatgc cgacggcgcc gaagttcccg cacgcgcctt
     1861 tgatggcgtg agcgacgagt aggcacgcgg cttcgtcggt ggacatcgcc gcgtcgcgca
     1921 gggctcgcac gcgcttcgtg atgtcctcga ggaagatttg ggtcagcagc accgccacgt
     1981 ccgacgagcc ttcgtcgaag gcgagctcct ccagccgaga gagatcgagg tgccccgtcg
     2041 tcgagtgggt gggggcgacg gtcgggggca cttcggcgct tgcaggccga gagtcacgac
     2101 cgcgcgttat ccaatacgcg acccgcgccc tcagggtggc gagagtgaaa ggcttcgaga
     2161 tgtagtcgtc catgcccgcc gcgaggcagt tgcgacgcgt ctcggcgacg ccgcccgcgg
     2221 tcacggcgat gattggtacg cggcgttttc cccgctccat ccgccggatt tcgcgcgtcg
     2281 tttcgaaccc gtcgaggccg ctcatgtggc agtccatcag cacgacgtcg tagtcgcgac
     2341 tgccgaagcg gttcagggcg tccatcccgt tgctcaccgc atcgacgtgg cagcccgtcg
     2401 ccgagagggt gtgcgtgatc agggcctgat tgaactcgtt gtcctcgacg acgagcgcgt
     2461 agccgccgac ttcggcggat tgctccggcg cttcgctcga gggcacgatg ctccgagtgc
     2521 tgtcgcgggg ctcgtccaac acctgattga tgatggagag cagctcctgg ccggagcggt
     2581 gaactccctt gaccgcggcg cgctgttccg cgtcgaggtt cgtctcgagc aggagctcgg
     2641 tcgcgccgat gacgacagtc atcggggtgc ggagctcg
//
LOCUS       DASTKS010001251         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1990534, whole genome shotgun sequence.
ACCESSION   DASTKS010001251 DASTKS010000000
VERSION     DASTKS010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990534"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..571)
                     /locus_tag="VFQ35_26745"
     CDS             complement(155..571)
                     /locus_tag="VFQ35_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934341.1"
                     /translation="MTEKQLNILVVDDDEVDVMNVQRAFKKGNIKNPLFVASDGIHGL
                     EVLRGDEFPKTNRLVLLDLNMPRMNGIEFLRELRADPELKGTSVVVLTTSNDNRDKVD
                     AYNLNVAGYLTKPVTFGNFVDLMVTLNKYWMLVELT"
     gene            complement(568..2670)
                     /locus_tag="VFQ35_26750"
     CDS             complement(568..2670)
                     /locus_tag="VFQ35_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934342.1"
                     /translation="MRTGNSTASLFHGGGQSGALMQSMNWAATPLGPVEKWPESLRAM
                     IPAMLASRFAMRVVWGPDYVMLYNDAYMPVLGRDKHPHAMGAPTAKSFPELWEIVEPL
                     FQRVYRGEAVALNDAHLPVHRNGYLEECYFTLSYSPMRDDMGAIAGLLGVISETTERV
                     LAERRLRTLRALSAHAASTAGAALTEVGAGALTTLGQNAADVPYSLLYLVEEDERARL
                     IGWHGLERGTRGAPEMLELADPQALWPLQSAAAQRETLTTPVESFGALPGGVWSEPSP
                     RAAVVSVARAGVRAYLVVGLSPRRAFDPAYADFLDLAADQIAAMLAGARAHEREQAAL
                     AEAHRQRSRLESLFMQAPAAICVLRGAEHVYEFANPRYLELIGNRDVVGRSMREALPE
                     AAPVVVPLLDGVFTRAEPYFGSELAVMLGRNGHPEECYFNFIYQPIFDAQGKVEGIAV
                     VAFEVTDQVRARQRSEALAQALEATNRDLDQFAYVASHDLKAPLRGIATLSEWIEEAI
                     SSQISGETRHQLDLLRGRVRRLEALIDGILDYSRAGRVREKIEAVNVGALLAECRDLL
                     APGPGAVIEIAPKMPTLMAERVPLQQIFMNLISNALKHAGRPDVRVEIGCTEVGASYD
                     FTVRDDGPGIPPQFQDRIWGIFQTLQPRDRVEGTGIGLSIVKKLVESRGGRVSIESAP
                     GAGATFHVLWPKRIKVSP"
     gene            2886..>4255
                     /locus_tag="VFQ35_26755"
     CDS             2886..>4255
                     /locus_tag="VFQ35_26755"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9934343.1"
                     /translation="MTTLSPAAIPGIPASMKGEMAELMRAWGWDDTPLGPPETWPEML
                     RSALSICLGTRFPIAIYWGERLALIYNDAWRPILGSKHPWGLGRAARDVWPEIWDAIG
                     PLFAQVVGTGVGTYSEDQLLLMYRHGFTEECYFNFTFSPILGSDGRVGGIFNAVIETT
                     ERVVGERRLRTLRALGEQAVFGVSVEAACTAAAATLGENSADLPFAMLYLADDPREPL
                     RLAASTGLPEKAAVAAVGLGASDVLAEELARASSGELVTVPASPERFGQLPGGAWPEP
                     PRELVILPLARAGQAGHLGFLVAGANPRRPLDEGYKGFLRLVAGHLAALIAGARAFEE
                     ERQRAEQLAELDRAKTTFFSNVSHEFRTPLTLMLGPLDDLLHDPSLEPSRREQLTLVQ
                     RNGLRLQKLVNSLLDFSRIEAGRVRAAFEPLELATYTAELASTFRSAMQKAGLELEVD
                     CAPLAEP"
BASE COUNT          716 a         1421 c         1370 g          748 t
ORIGIN      
        1 aaacggctac caatccttga actgggacta ctacctggct ctcaccaatt gcatcgaacg
       61 cttggaggaa gcgcttccac gttgagcgcg ctgcctcgac gcgagcgggc tgctcgacgt
      121 gagcggcccg ctccgtggtc gttgccagcg ggcactacgt gagctcgacc agcatccagt
      181 acttgttcag cgtgaccatc aggtccacga agttcccgaa ggtgaccggc ttcgtgaggt
      241 acccggctac gttgaggtta taggcgtcga ccttatcgcg attgtcgttc gaggtcgtca
      301 acacgacgac gctcgtgccc ttcagctcgg gatcggcgcg tagctcccgc aagaactcga
      361 tgccgttcat gcgcggcatg ttgaggtcca ggagcacgag tcggttcgtc ttcgggaact
      421 cgtctccccg cagcacttcg aggccgtgga tcccatcgct cgcaacgaag agcgggttct
      481 tgatattgcc ctttttgaac gcgcgttgga cgttcatgac gtcgacctcg tcgtcatcga
      541 caacgaggat attgagctgc ttctcggtca cggagatacc ttgattcgct tgggccaaag
      601 gacgtgaaac gtggcgccag caccgggcgc cgactcgatg ctaacccgac caccgcggct
      661 ttcgaccagc ttcttgacga tggacaagcc gatcccggtt ccctcgacac ggtcacgtgg
      721 ctgcaaagtc tggaagatgc cccagatcct gtcttgaaac tgcgggggga tgccggggcc
      781 gtcgtcccgg accgtgaagt cgtacgaagc gcccacttcg gtacacccga tttcgacacg
      841 cacgtctggc cgcccggcgt gcttcagcgc gttgctgatc aggttcatga agatctgctg
      901 caatggcacg cgctcggcca tcagggtcgg catcttcggc gcgatctcga tgacggcacc
      961 gggacccggc gcgaggaggt cgcggcattc ggcgagcagc gctccgacgt tcaccgcttc
     1021 gatcttctcg cgcacgcgcc ccgcgcgcga atagtcgagg atgccgtcga tcaacgcctc
     1081 gagtcggcgc acgcgcccgc gcagaaggtc caattggtgc cgcgtctcac ccgaaatctg
     1141 gctcgaaatg gcctcctcga tccattcgga gagggtcgcg atcccacgga gcggcgcctt
     1201 cagatcgtgc gatgcgacgt acgcgaactg gtcgaggtcg cggttggtcg cttcgagcgc
     1261 ctgggccaga gcctcgctcc gctggcgtgc ccgcacctga tccgtgacct cgaatgcgac
     1321 gacggcgatc ccctccacct tgccctgggc atcgaagatc ggctggtaga tgaagttgaa
     1381 gtagcattct tctggatggc cgtttcgccc gagcatcacc gcgagctcac tgccgaagta
     1441 cggctcggct cgcgtgaaga cgccgtcgag gagcggcacc accaccggcg ccgcctcggg
     1501 cagagcctcg cgcatcgagc gaccgactac gtctcgattg ccgatgagct ccagatagcg
     1561 tgggttcgcg aactcgtaga cgtgctcggc cccccgcaac acgcatatgg cggcaggagc
     1621 ctgcatgaac aatgactcga gacgcgaccg ttgtcggtga gcctccgcga gcgcggcctg
     1681 ctcacgctcg tgggctcggg cgccggccag catcgccgcg atctggtccg cggcgaggtc
     1741 gaggaagtca gcgtacgccg ggtcgaatgc tcgccgcggg ctgagaccca cgacgaggta
     1801 ggcgcgcact cccgcgcgcg cgactgatac cactgccgcc cgcgggctcg gctcgctcca
     1861 gaccccaccg ggcagcgcgc cgaacgactc gaccggtgtc gtcagcgttt cgcgctgcgc
     1921 ggcggccgac tgaagaggcc agagcgcctg cggatcggcg agttcgagca tctcgggcgc
     1981 gccccgcgtg ccgcgctcca agccgtgcca cccgatcaat cgggcccgct cgtcttcctc
     2041 gacgaggtac aagagcgagt acggtacgtc cgccgcattc tgaccgaggg tcgtcagcgc
     2101 gcctgctccg acttcggtca aggcggctcc cgcggtcgac gccgcgtgcg cggagagcgc
     2161 gcgcagagta cgcagacgtc gctcggcgag cacccgttcg gtggtctcgc tgatgacgcc
     2221 caggaggccg gcgatggctc ccatatcgtc gcgcatcgga ctataggaga gcgtgaagta
     2281 gcactcctcc aagtagccgt tgcggtgcac gggtagatgc gcgtcgttca gcgcgaccgc
     2341 ttccccccgg taaacgcgtt gaaacagggg ctcgacgatt tcccaaagct cggggaagct
     2401 cttcgcggtc ggcgcaccca tcgcatgcgg gtgcttgtcg cgaccgagca ccggcatgta
     2461 agcgtcgttg tagagcatca cgtagtcggg cccccacacg acgcgcatcg caaagcgcga
     2521 agcgagcatc gccgggatca tcgcgcgcaa gctctcgggc cacttttcga ccggcccgag
     2581 cggcgtggcg gcccagttca tcgactgcat cagcgcgccg ctctggccgc cgccatggaa
     2641 caggctcgct gttgaattgc cagtacgcac gccgcaattc ttatactagt tgccgcaccg
     2701 cgagccacgt cgaagctcag gcgacgcgaa ctcgatgctc ggaccggcag cgctgtggca
     2761 tcccgccaca tgcgacagat cgccacgtcg aactgccgac tgctcgaata ccctaattcg
     2821 cttttgctca agattcgttc gcaacaacgc ttgtcgcgtg cgagagtcca tcggctcgcg
     2881 acttcatgac gacgctatct ccagccgcga ttccaggaat cccagcatcg atgaaaggcg
     2941 agatggccga gttgatgcgc gcgtgggggt gggacgacac gcccctcggt ccgcccgaga
     3001 catggccgga aatgctgcgt tctgcgctca gcatctgcct cggtacgcgc tttccgatcg
     3061 cgatctactg gggcgagcgg ctcgccctca tctacaacga cgcctggcgt ccgatcctcg
     3121 ggagcaaaca cccttggggg ttgggacgcg ctgcccgcga cgtgtggcca gagatttggg
     3181 acgcgatcgg accgttgttc gcgcaggtag tcggcacggg agtcggcacg tactcggagg
     3241 accagctcct cttgatgtat cgacacggtt tcaccgaaga atgctacttc aacttcacct
     3301 tcagtccgat tctgggtagc gacgggcgcg tcggcggcat cttcaacgcc gtgatcgaga
     3361 ccaccgagcg cgtggtcggc gagcggaggc tccgcaccct gcgcgcgctc ggcgaacagg
     3421 ccgtgttcgg ggtctcggtc gaggcagcgt gcacggcggc agcagcgacc ctcggagaaa
     3481 actccgctga cctcccgttc gcgatgctgt atctcgccga cgatccgcgc gagccgcttc
     3541 gcttggccgc ttcgaccggg ctgcccgaga aagccgccgt ggccgcggtc gggctcggcg
     3601 cgagcgacgt gctcgccgaa gagctcgcgc gcgcgagttc gggtgagttg gtcaccgtcc
     3661 cggcctcgcc cgagcgcttc ggtcaattgc ccggcggagc gtggccggag ccgccgcggg
     3721 agctcgtgat tttgccgctc gcacgcgcgg gccaggcggg gcaccttggg ttcttggtgg
     3781 ctggggcgaa tccccggaga ccgctcgacg aaggctacaa gggcttcctg cgactggttg
     3841 cggggcacct cgcggcgctg atcgcgggcg ctcgcgcgtt cgaagaagag cgacagcgcg
     3901 ccgagcaact agccgagctc gatcgcgcga aaaccacgtt cttctccaac gtgagccacg
     3961 agtttcggac tccgctcacg ctcatgctcg gtccgctgga cgatctgctt cacgaccctt
     4021 cgctcgagcc gtcgcgacgt gaacagctca cgctggtgca gcgcaacggg ctccgactcc
     4081 aaaagctggt caattcgctg ctcgacttct cgcgcatcga ggccggacgc gtgcgcgcgg
     4141 cgttcgagcc gctcgagctc gccacgtaca cggcggagct cgcgagcacg ttccgctccg
     4201 caatgcagaa ggcggggctc gagctcgaag tcgattgcgc cccgctcgcc gagcc
//
LOCUS       DASTKS010001252         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1095292, whole genome shotgun sequence.
ACCESSION   DASTKS010001252 DASTKS010000000
VERSION     DASTKS010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095292"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFQ35_26760"
     CDS             <1..222
                     /locus_tag="VFQ35_26760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934344.1"
                     /translation="SAATECYWLGMVQKVAKANSSNTGQSGRRGVRPSSDREPFGVAL
                     VDALVAADARLQRGERSVGLGYLLQRAAH"
     gene            260..1075
                     /locus_tag="VFQ35_26765"
     CDS             260..1075
                     /locus_tag="VFQ35_26765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934345.1"
                     /translation="MTTESPAESYSPNDDGPTRISARTLVVVPCYNEEKRLDTAAFLA
                     ALSQEQNLEFVFVNDGSADGTQTILEEAARRGGPRVHLVALPENQGKAAAVRAGVLRA
                     FELGAEIIGYWDADLATPLAAIGAMSQAFRDPHILLVLGSRVQLLGRHIKRDPMRHFI
                     GRGFATAVGLALGMPVYDTQCGAKLFRASPIMKRAFEESFEHVWTFDVELLSRLLKEE
                     ARAGTLHLSEQCVEFPLAAWHDKPGSKIRVTHVPRIAWEVIRFFVYSPRWSRR"
     gene            complement(1148..>2280)
                     /locus_tag="VFQ35_26770"
     CDS             complement(1148..>2280)
                     /locus_tag="VFQ35_26770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9934346.1"
                     /translation="LSVCALQACSDDSDTNAAREAAFPQSASAELQHTLDDVVHAGVA
                     PGVSAVVKRPGYAEWAGAAGSANIQTSIALTPGARFRAGSIMKVAVATAVLQLVERGL
                     LSLDDVLTKRLPNELAARIPNADSITVRMLLNHTSGIPEFADDAFHEQVVAAPAHVWS
                     SAEFYDLALAKPPLFAPGTSWAYSNTDYILLGDIMEGVTHEPWRQTVRHDVFTRAHLS
                     TSSLPEPGDASCSGCARGYEPIGDQLVDLTEIDPSMAGAAGGEAFVTTPSDLAKLVSE
                     LARGALYDDPHTLELMLSFADAPVPQETQVGYGLGIARFQMGDVELIGHLGGTAGFQS
                     FVFYQPSSGTTVSGYMNRQGDFGAFVLPFMTTVGKTADTPAK"
BASE COUNT          402 a          719 c          768 g          391 t
ORIGIN      
        1 tcggccgcga ccgaatgcta ttggctcggc atggttcaaa aggtggccaa ggcgaactcg
       61 tcgaacaccg gacagagcgg ccgtcgaggc gtgcgcccga gctccgaccg ggagccgttc
      121 ggcgtcgcgc tggtcgacgc gctcgtcgcg gccgatgcgc ggctacagcg cggcgagcgt
      181 tcggtcgggc tcggatatct cttgcaacga gcggctcatt gaaagcgtcg cgcagtgctt
      241 cattcgtcga ggtcctcgaa tgacgaccga gtcgccggct gagagctact cgcccaacga
      301 tgacgggccg acccgcatca gtgcgcgcac gctcgtggtc gtcccctgct acaacgaaga
      361 gaagcggctc gatacggcgg cgttccttgc ggcgctttcg caagagcaaa acctcgagtt
      421 cgtcttcgta aacgacggca gcgccgacgg cactcagacg attctcgagg aggcggctcg
      481 gcgtggcggt ccgcgcgttc acctcgtcgc gttgcccgaa aatcaaggca aggccgcggc
      541 ggtgcgcgcc ggcgttctgc gcgcgttcga actcggcgcc gaaatcatcg gctactggga
      601 tgcagacttg gcgacccccc tcgccgcgat cggagcgatg tcgcaggcgt tcagagaccc
      661 gcacatattg ctcgtgctcg gctcgcgcgt gcagctcctc ggtcggcaca tcaagcgcga
      721 tcccatgcgg cacttcattg gtcgcggctt cgccactgcg gtgggcctcg cgctcgggat
      781 gcccgtctac gacactcagt gtggggccaa gctgtttcgc gcaagcccca tcatgaagcg
      841 agccttcgag gagtctttcg agcacgtctg gacgttcgac gtcgagctgc tgtctcggct
      901 cttgaaggag gaagcacgcg cgggaacgct ccatttgagc gagcagtgcg tcgaatttcc
      961 gctggcggct tggcacgaca agcctgggtc gaagatcagg gtcacgcacg tccctcgtat
     1021 cgcgtgggaa gtcatcaggt tcttcgtcta ctcaccgcgt tggtcgcgac gctgacgccg
     1081 gcgttcatcg tcgagctttc tcgagtgcgc gcgcgatcag ttcagcgcgc gcaccgaaaa
     1141 gcgcagctca cttcgcgggc gtgtcggcgg tcttgccgac cgtcgtcatg aacggaagaa
     1201 cgaaagcgcc gaagtcgcct tgccgattca tgtagccgct gacggtcgtc cccgaactcg
     1261 gctggtagaa gacgaagctc tgaaagccag cggtgccccc caaatgcccg atgagctcga
     1321 cgtcgcccat ctggaagcga gcgatgccga ggccatagcc tacctgcgtc tcttgaggca
     1381 cgggcgcatc cgcgaagctc agcatcagct cgagggtgtg gggatcgtcg tacagcgcgc
     1441 ccctcgcaag ttcactcacg agcttggcca gatccgaagg cgtcgtcacg aacgcttcgc
     1501 cgccggcagc gccggccatc gacggatcga tttcggtcag atcgacgagc tgatctccga
     1561 tgggttcgta gccacgggca cagccggagc agctcgcgtc accgggctcg ggtagcgagc
     1621 tcgtcgaaag gtgagcgcgg gtgaacacgt cgtggcgaac cgtctgtcgc cacggctcgt
     1681 gcgtcacccc ttccatgatg tcgccgagca agatgtagtc ggtgttggaa tacgcccagc
     1741 tcgtgccggg cgcgaaaagc ggcggtttgg caagagcgag gtcgtagaac tcggcagacg
     1801 accaaacgtg cgcgggggct gccaccacct gctcgtgaaa agcgtcgtcg gcgaactcgg
     1861 gaatcccgga ggtgtggttc agcagcatgc gaaccgttat ggagtcggcg ttcggaatgc
     1921 gcgcggcgag ctcgttcggc aagcgtttgg tgagcacgtc gtcgagtgag agcaacccgc
     1981 gctccacgag ctgaagcacg gcggtggcga ccgcgacctt catgatgctg cccgcgcgaa
     2041 agcgggcgcc gggagtcagc gcgatcgaag tctggatatt cgccgagcca gcggcgccgg
     2101 cccactccgc gtaaccgggg cgcttcacga ctgcggacac cccgggcgcg acccccgcgt
     2161 ggacgacgtc gtcgagtgtg tgctggagct cagccgaggc ggactgtggg aacgcggcct
     2221 cccgagcggc attcgtatcc gagtcgtcgg aacaagcctg gagagcgcag acgctgagag
//
LOCUS       DASTKS010001253         5048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1543559, whole genome shotgun sequence.
ACCESSION   DASTKS010001253 DASTKS010000000
VERSION     DASTKS010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543559"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFQ35_26775"
     CDS             <1..1021
                     /locus_tag="VFQ35_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9934347.1"
                     /translation="TGLFTSGPASPDTYRAVRGKSVIDLVRDDLDTLERHDMSGADRS
                     KLEAWKELLDATGTVVKREHCDPEIAAQLSLTEDNVRAFTTSGDPLCTKIAGTNFDGA
                     DLYSHLAVLAALCNANPIIVLKYPTAYLFKGLGLTTEAASLANRIGNAGMNGPCLANV
                     IDMIATIDDYYTRKFAHLGQLLNSFDEGEGTLLDNSAAVWFQDVSDGSAKNHNNLPIV
                     QLGSAGGYFRTGWAVNVEDGAPNLTAGNSESVCAPGTPDMADGVNQVTGTDPKLASAP
                     INKYFCNLMNALGVKGGPDGFPLKGGSAEVTHFGMYDRTEDFVGGGTNPPKIRSPGEY
                     TVLKA"
     gene            complement(1052..1672)
                     /locus_tag="VFQ35_26780"
     CDS             complement(1052..1672)
                     /locus_tag="VFQ35_26780"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9934348.1"
                     /translation="MLRTGLVGSVLLGLSIAGCAKKSEPEAPALHAAVTESAAPAVQA
                     AAPANAAPAPSSALLQVGASLPDVDGVAQNGERVNLKSLAGKPIVVYFYPKDDTPGCT
                     VEAQEIRDAYKELSGTGAVIIGVSVDDAASHKAFAEKHALPFLLVPDADQRWVKAFGV
                     PLLPNGRASRVSFVFGRDGKLAKVFPDVKPQGHAQELGEALKTASK"
     gene            complement(1842..3350)
                     /locus_tag="VFQ35_26785"
     CDS             complement(1842..3350)
                     /locus_tag="VFQ35_26785"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934349.1"
                     /translation="MQDLPSVGQLVAGKYRIERVIGSGAMGVVMSAWHVELLQRVAVK
                     FVRPEAMGLRDAEERFRREARAAARIRSEHVGRVMDVGTVEGGVPYMVMEYLEGHDLA
                     DELSRRGPLAVAEAVGYLLEAIEALAEAHAAGIVHRDLKPANLFLAKRADGTRIVKVL
                     DFGVSKAGGTRPGDMALTQQAVMIGSPLYMSPEQMRSARDVDARADIWSLGVILFELV
                     GGRPPYLGDSIPELVQSMMEAPPSLRALRGELPLALEAVVARCLERDPSDRFADVASL
                     GAALVEFAPDLRMHVTRAQRLLGSAPLLGAEPRFDTLAASQPSGRRTVAAWGEAQPPS
                     ERRSSRRVFAGLGVLTVLVGALGVGYTRFARTSFGVSVPAVAAALVPASLSSAAGIGA
                     VVAVPGPSAKVATVPPKLDAPSGASAPSPWGASGASAPSGGVAPAASTEASSALAAVP
                     APVSSVVAAPKPPQRPPPRYVAPRRPAAQSMPAEAPHSPAVKDTLPDFGGRR"
     gene            complement(3392..3835)
                     /locus_tag="VFQ35_26790"
     CDS             complement(3392..3835)
                     /locus_tag="VFQ35_26790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934350.1"
                     /translation="MLHRSFRRAVGAACAAALAIACASSDSGTSEKTPSFCDVRPILE
                     SKCQRCHQDPPLHGAPFALVTYADTQADAATPSAPSRKRYEQMRVAVENDTMPDRSQT
                     LDPPVSPLSCEEKATLLAWLRKGAPPAPDGEDGCKSGSSELLSCP"
     gene            complement(4095..>5048)
                     /locus_tag="VFQ35_26795"
     CDS             complement(4095..>5048)
                     /locus_tag="VFQ35_26795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934351.1"
                     /translation="LLATRKGKLVGRITAHVDRAYNEYHDEKAGWFGFFDCIDDVGVA
                     HALLDRAVRWVHAAGMKEVIGPCNFTTNHQVGMLVENFSRPAVVEMTYNASYYERLVT
                     SYGFGKAKDLLAYWIDVSQGVDDPKVKRFYDVSQKAQKRYGLTLRGARMKDFDDEVAR
                     LFRLYNDTWQKNWGFVPVSEPEFKNIAKDLKQVVAPDLVLIVEDKSGTPVAFSVTLPN
                     INEIMPRNGRLFPFGWWRLLTGMKSIKTARLFTLGVMPAHRRHGVEAMLCIETALRAK
                     RLGYSSGEIGWTLEDNILINRTVDSFGGKLDRRYRLFGLAL"
BASE COUNT          903 a         1697 c         1595 g          853 t
ORIGIN      
        1 caccgggttg ttcacgagcg gccctgcttc tcccgacacc tatcgagcgg tgcgtgggaa
       61 gagcgtcatc gacctcgtcc gagacgacct cgacacgctc gagcggcacg atatgagcgg
      121 ggccgatcgg agcaagctcg aggcctggaa ggagctgctc gacgcgactg gcaccgtcgt
      181 caagcgcgag cattgcgacc ccgaaatcgc ggcgcagctg agcctcacgg aggacaacgt
      241 gcgcgcattc acgacgagcg gcgatcctct ctgcacgaag atcgcgggaa ccaatttcga
      301 cggcgccgac ctctactcgc acctcgcggt gttggccgcc ctctgcaacg caaacccgat
      361 catcgtcttg aagtacccga ccgcgtatct cttcaaaggg ctcgggttga cgaccgaggc
      421 cgcgagccta gcaaatcgta tcggaaacgc ggggatgaac gggccttgcc tcgccaacgt
      481 gatcgacatg atcgcgacga tcgacgacta ctacactcgc aagttcgcgc acctcggcca
      541 attgttgaac agcttcgatg aaggcgaagg cacgctgctc gacaactccg ccgccgtatg
      601 gttccaggac gtgtcggacg gcagcgccaa gaatcacaac aatctgccga ttgtgcagct
      661 cgggagcgcg ggcgggtact ttcgcaccgg ttgggccgtg aacgtcgaag acggcgcgcc
      721 caacttgacc gcaggcaaca gcgagtcggt gtgcgcgccc ggcacccccg acatggccga
      781 cggcgtgaac caggtcaccg gcacggatcc gaagctcgcg agtgcgccca tcaacaagta
      841 tttctgcaac ctgatgaacg cgctcggggt gaagggcggg cccgacgggt tcccgctcaa
      901 aggcggcagc gccgaagtga cgcacttcgg catgtacgat cgcaccgagg acttcgtcgg
      961 cggaggcacg aatccgccaa aaatccgcag ccccggcgag tacaccgtgc tcaaagcctg
     1021 accaagcccg cgtgcgcgcg ttgcgctcag ctcacttcga agctgttttc agcgcctcgc
     1081 cgagctcctg ggcgtgccct tgtggcttca cgtcggggaa caccttggcg agcttgccgt
     1141 cacgcccgaa cacgaagctt acgcgcgacg cgcgcccatt cggcaatagc ggtacaccga
     1201 aggctttcac ccagcgctgg tcggcgtcgg gaacgagcag aaacggaagc gcgtgctttt
     1261 cggcgaacgc cttgtgcgat gcggcgtcgt cgacggatac gccgatgatc acggcgccgg
     1321 tcccggacag ctccttgtag gcgtcacgaa tttcttgagc ttccacggtg cagccgggcg
     1381 tgtcgtcttt cgggtagaag tagacgacga tgggcttgcc ggcgagcgac ttcaggttca
     1441 cgcgctcgcc gttttgcgca acgccgtcga cgtctggcag cgacgcgccg acctgcagca
     1501 gcgcggagct cggcgcgggc gcggcgttcg ctggggcggc ggcttgcacc gcgggcgcag
     1561 cgctctccgt cacggccgcg tgcagcgcgg gcgcttctgg ttcgctcttc ttcgcgcaac
     1621 cggcgatcga tagaccgagc agaactgaac ctacgagtcc ggtgcgcaac atgggcccga
     1681 acctagcacg tcgctgcgct cctcgacatg cgcgggccct aacagggcgt tgaaaaatac
     1741 cctgttagaa tgctgaccgg caccaacgtg cagcttgctt ctgcgcgtcg tgtgtacctg
     1801 gcattggtct gtcgtggcgc agggcgtttt tcaaggccct gctaacgccg gccgccgaag
     1861 tccggaagcg tgtccttcac cgcaggggag tggggcgctt cggctggcat tgattgagcc
     1921 gcgggacggc gaggagctac gtatctcggc ggggggcgtt gcggcggctt cggagctgcg
     1981 acgacgctcg acacgggagc cggaacagcc gcgagggcgc tcgaggcctc ggtgctcgca
     2041 gctggcgcaa cgccaccgct cggagccgat gcgccacttg caccccatgg gctcggagcc
     2101 gatgcgccac tcggcgcgtc gagtttgggc ggcaccgttg caaccttcgc cgaaggcccg
     2161 ggaaccgcga cgacggcgcc gatcccagcc gccgacgaca gcgatgcagg cacgagggcg
     2221 gccgcgaccg ctggcacgct cacgccaaag ctcgttcgcg caaaacgcgt gtacccgacg
     2281 cccagcgcgc cgacgagcac ggtgagcact ccaagcccgg cgaacacacg ccgggaagag
     2341 cgacgttcgc tgggcggctg cgcttcgccc caagctgcga cggttcgacg ccccgaaggc
     2401 tgggacgccg cgagcgtgtc gaaccgaggt tccgcgccga gcaggggagc acttccgagc
     2461 aagcgttgag cgcgtgtcac gtgcatgcgc aagtccgggg cgaactccac gagcgcggcg
     2521 ccgagcgacg cgacatcggc gaacctatca ctcgggtcgc gctccaggca gcgcgcgacg
     2581 accgcctcga gcgcgagtgg cagctcaccc cgcaaagctc gcagcgaggg cggcgcctcc
     2641 atcatggact gcacgagctc cggaatggag tcaccgaggt acggaggccg cccaccaacg
     2701 agctcgaaca ggatgacccc gagcgaccaa atgtccgcgc gcgcgtcgac gtctcgcgcc
     2761 gagcgcatct gctcgggtga catgtagagc ggcgagccaa tcatcaccgc ttgctgcgtg
     2821 agcgccatgt cgccagggcg cgttccaccc gccttcgaaa caccgaagtc gagcaccttg
     2881 acgatgcgcg taccgtccgc gcgctttgcc aggaacaggt tcgcgggctt cagatcgcgg
     2941 tgcacgatgc cggccgcgtg cgcctccgcc agcgcttcga tcgcctcgag caggtaaccg
     3001 acggcttctg ccaccgccag gggtcctcga cggctgagct cgtccgcgag atcgtgtccc
     3061 tcgaggtatt ccatcaccat gtaggggacg ccaccctcga cggtgccgac gtccatcacg
     3121 cggcccacgt gctcgctgcg aatgcgcgca gctgctcgag cctcgcgacg aaagcgctct
     3181 tccgcgtcgc gcagccccat cgcctcgggg cgcacgaatt tcaccgcgac ccgctgcagt
     3241 agctcgacgt gccacgccga catgacgacg cccatcgcgc cagaaccaat gacacgctcg
     3301 atccggtact tacctgcgac gagctgccct acactaggca ggtcttgcat cgaagaagct
     3361 agtatcgcac gcgctttttc gtcgccaagt atcaggggca cgagagcagc tcgctactcc
     3421 cacttttaca cccatcttcg ccgtctggcg ctggcggcgc tcctttccgt agccaggcga
     3481 gtaaagtcgc cttctcttcg cagctgagcg ggctcacggg cgggtcgagc gtttgcgagc
     3541 gatccggcat ggtgtcgttt tcgactgcaa ctcgcatttg ctcgtagcgc ttgcgtgaag
     3601 gtgcgctcgg agtcgccgcg tctgcctgcg tgtcggcgta cgtaaccaga gcgaacggcg
     3661 cgccatgcag cggtggatct tggtgacagc gctgacattt gctctcgagg atgggacgga
     3721 catcacaaaa gctcggcgtc ttctcactgg tgcccgagtc cgagctcgcg caggcgatcg
     3781 cgagcgccgc cgcgcaggcc gcccctacag cgcgtcgaaa cgaacggtga agcattacga
     3841 cgacacgcta gttcagcgca tgtgagatag cgtcaacttt ttcgccgtcg ctctcgggcg
     3901 cgtcccaagt tctccgccct gctgcgagcc atgatcctcc ggctgccagg cagtcacgcg
     3961 ggccgcttgc gcccacgcat ccccaccaga gcgcacacca cgagcccgcg acccacaccc
     4021 cagcactctc gcatcgagcc gctcgaggcg cgcgagcgcc tcttcgtcgt acggacttcg
     4081 agcgcggcga cggctcagag cgccagcccg aacaggcgat agcgccgatc gagcttgcct
     4141 ccaaacgagt cgacggtgcg gttgatgaga atgttgtcct cgagcgtcca gccgatctca
     4201 ccgctcgagt aaccgagccg tttcgcgcgc agcgcggttt cgatgcacag catcgcttcg
     4261 acaccgtgac ggcgatgcgc cggcatcact cccagagtaa atagccgcgc tgtcttgatg
     4321 ctcttcatgc cggtgagcaa gcgccaccag ccgaacggaa agagccgccc gtttctcggc
     4381 atgatctcat tgatgttcgg gagcgtcacc gagaaggcga ccggcgtacc gctcttgtcc
     4441 tcgacgataa gaacgagatc tggcgcaacc acctgcttca gatccttcgc gatgttcttg
     4501 aactcgggtt cgctcaccgg cacgaagccc cagttcttct gccaggtgtc gttgtaaaga
     4561 cgaaataggc gcgccacctc gtcgtcgaag tccttcatgc gcgcgccgcg cagcgtgagc
     4621 ccgtaacgct tttgcgcctt ttggctgacg tcgtagaagc gcttgacctt cggatcgtcc
     4681 acgccctggc tcacgtcgat ccagtacgcg agcaggtctt tggctttgcc gaagccgtag
     4741 ctcgtaacca gtcgctcgta gtagctcgcg ttgtacgtca tttcgacgac ggccggccgc
     4801 gagaagtttt cgaccaacat tccgacctgg tggttggtgg tgaagttaca ggggccgatc
     4861 acctctttca tgcccgctgc gtgcacccag cgcacggcgc ggtcgagcag agcatgcgcg
     4921 accccgacgt cgtcgatgca gtcgaagaag ccgaaccagc ccgccttttc gtcgtgatac
     4981 tcgttgtagg ctcggtcgac gtgagcggtg atgcgcccca cgagcttccc tttgcgcgtc
     5041 gcgagcag
//
LOCUS       DASTKS010001254         6683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_203118, whole genome shotgun sequence.
ACCESSION   DASTKS010001254 DASTKS010000000
VERSION     DASTKS010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203118"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VFQ35_26800"
     CDS             <1..1327
                     /locus_tag="VFQ35_26800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934352.1"
                     /translation="SGKGPRGLGALLLESEQGLADLLRDKGLSDMPAREAFGNVSGDA
                     TLERVAVFGHYLTISGSRFRAGKEFYFADLGVSGAEMVQKLKLADFDGDGKQEIVMVK
                     RFGAQDKYREVLEVTRTGRDDTPFVAFSHEVGIKTPDGSISNKVKIEDSGIEISQGDA
                     EGFEPDTYNEPLPGGNTLSALFPWESVASRTFKWQGNGFVKASEEKQTPKVSKSSSRS
                     AGPSSDEPAQPAAPRAPSADELLDRVYALYKKDRGTGGKARFDFVADVAGDSAPERVL
                     IHDKDVVVFGKGFRSGTSYAFITLGVSDPKDIRDATARDLTGDGKAEIIVRAVMRAKA
                     SKELGGETIDRHALLVYSVSGDKLVRVFAAETGRSLGKNQVTGSVAFEPSGKAVRIEL
                     RPGRAVGWTESSYPFPQDTTPAGGLEPLLLPWTGGKRTYRFNGSAFVRE"
     gene            1411..1761
                     /locus_tag="VFQ35_26805"
     CDS             1411..1761
                     /locus_tag="VFQ35_26805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9934353.1"
                     /translation="MTTTPSGLSYEDTTLGNGASPEKGQTCVMHYTGWLWVNGAKGSK
                     FDSSVDRGRPFEFPIGRGRVIKGWDEGVASMKVGGKRTLLIPPDLGYGASGAGGVIPP
                     NATLLFEVELLALR"
     gene            2459..3106
                     /locus_tag="VFQ35_26810"
     CDS             2459..3106
                     /locus_tag="VFQ35_26810"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET9934354.1"
                     /translation="MRRTERLFALTEYLRGRRSGVTAEALAERFGVTIRTIYRDLDAL
                     RDASLPVAAEPGRGGGYALEKSYSLPPVNFTAREAALLVALGRFASDMRLLPFTETLE
                     SALDKVRAALSASAQRELLQRLQELSFIGVPALPAEKSVRAAIERAWFERQPLAIVAL
                     EQGYIETKCRVRIQSVVMARHDTRIQGLDLDDGSQRNFRLDRILKAEVLELDSHK"
     gene            3774..6143
                     /locus_tag="VFQ35_26815"
     CDS             3774..6143
                     /locus_tag="VFQ35_26815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9934355.1"
                     /translation="MSTLERKLYRDLFQLKAQIASISLVFASGIVCLIAMRGCFIAME
                     RAVDEFYERYRFAHVFAHASRVPVSVAPHIEALAGIATVEARIAEDASLPLPGLRQPA
                     YGRIISLPEGRQPSANALHLKRGRTPASGRSDEVVVLEAFANAHGLEPGAEVPIVLNG
                     KQRSMRIVGIALSPEFIYSIRPGALVDDPKRHAVLWMTQSAVTAAFGLSGCFNDVALR
                     LEPGASEAAVRAALDRVLVPYGGTGSFSRRDQVSHRAVTQQLGQLRVLSTTVPVMFLS
                     VAAFLVNVVLGRLIRLQRSAVALLKALGYTNREIARHYAGYVFSIVVPGSGLGLVGGA
                     LLGRHMLRLYAEFFRLPSLSLEFHVPPALVGYALVASSIAALAGALGAVRSAILLPPA
                     EAMQPASPPRYSRSVFERLGLGVLAGTSGLMVFREIGRRPLRTVFSALGMAGAVSLLV
                     LSRFGFDSLNAYFERTFRREQRQDIVVSFRGPAPPRVVDELARVPGVVSAEGVRAVPI
                     RIRHEHRNRDTVLLGIAPGSTLRVLVSRNGARVPVPPDGIIVEQTLASRLGLRLGDRP
                     ELEVHEGERQTLTPVIVGFIDETAGLGVYAQAPFVASLIGDVGAVTSALLTVDPLQLE
                     EVYRRLHRSPWILDVSDTKEEMNQMRDMNTAFINVWTAVSVVLAGGIVFGVVYNNARI
                     GLAARARDLATLRVLGFSRRQISGILLGSEAFEVALALPVGLWLGAALARAFMENIDP
                     EALRWNVVISPSTHFAATTLVLALALGSALWVRRRVDSLVLTDVLKARE"
     gene            complement(6275..>6683)
                     /locus_tag="VFQ35_26820"
     CDS             complement(6275..>6683)
                     /locus_tag="VFQ35_26820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934356.1"
                     /translation="EATRVCSDGSTIVGAVYVGFDLSTVLNGAPGFWREGGGMPLGAG
                     VPSGCSADGRVQAGYVEQWYYPMLIGPPRLIYASIYVDDGSSTNWAELIKNSPDAAGW
                     TLNSVAGISDDGKVLAGDGRHDGHDEGWVAHLP"
BASE COUNT         1204 a         2055 c         2182 g         1242 t
ORIGIN      
        1 gagcggcaaa ggtccgcgcg gcctcggcgc gctgctcctc gagtccgagc agggcctcgc
       61 cgatctgctt cgggacaaag gcttgtcgga catgcccgcg cgcgaagcat tcggcaacgt
      121 gagcggcgac gccacgctcg agcgcgtcgc ggtgttcggc cactacttga ccatcagcgg
      181 ctcacgcttt cgcgccggta aagaattcta cttcgcggat ctcggcgtga gcggggccga
      241 aatggtccaa aagctcaagc ttgccgactt cgacggcgac ggcaaacaag agatcgtcat
      301 ggtcaagcgc ttcggcgctc aagacaagta ccgcgaagtg ctcgaggtca cccgcaccgg
      361 ccgcgatgac acgccgttcg tcgccttctc gcatgaagtt gggataaaga ctccagacgg
      421 ctcgatctcg aacaaggtga agatagaaga cagcggaatc gagatttcgc agggcgacgc
      481 agagggcttc gagccggaca cgtacaacga gccgttaccg ggcgggaaca ccttgtcagc
      541 cctgtttccc tgggaatcgg tcgcgagccg taccttcaaa tggcagggca acggcttcgt
      601 caaagcgagc gaagaaaagc agacgcccaa agtgagcaaa tcgagctcgc gtagcgcggg
      661 accgagcagc gacgagccgg cgcaaccggc cgctccgcgg gctccgagcg ccgacgagct
      721 tctcgatcgc gtgtacgcgc tctacaaaaa ggatcggggc actggcggca aagcgcgctt
      781 cgacttcgtc gccgacgtcg ctggcgatag cgctcccgag cgcgtcctca tccacgacaa
      841 ggacgtcgtc gtgttcggca aaggctttcg ctccggcacg agctacgcgt tcatcacgct
      901 cggcgtctcg gatccgaaag acattcgcga cgccaccgcg cgcgacctca ccggagatgg
      961 aaaagcagaa atcatcgtgc gagcggtgat gcgcgccaaa gccagcaaag agctcggtgg
     1021 tgagaccatc gatcgccacg ccttactcgt ctacagcgtc agcggggaca agctcgtccg
     1081 tgtcttcgcg gcggagaccg gtcggagcct cggcaaaaat caggttaccg gcagcgtggc
     1141 cttcgaaccg agcggcaagg cagtgcgcat cgagctgcgt cccggacgag cagtgggctg
     1201 gacggagagc agctacccgt tcccgcaaga tacgacgccg gctggcggtc tcgagccttt
     1261 gcttttgcct tggactggcg gcaagcgcac ctaccgcttc aacggttctg ccttcgttcg
     1321 cgagtgagca gcggggcctc gaactgctcc cgtgagcgac atccgccgct tgacctcggc
     1381 tctcggatct gaaacgagga atgtatggcg atgaccacca ccccgtccgg gctctcttac
     1441 gaagacacta ctcttggcaa cggcgcgtcg cctgaaaagg gccaaacgtg cgtcatgcac
     1501 tacacggggt ggctttgggt caacggggcc aagggcagca agttcgacag ctcggtcgac
     1561 cgtgggcggc cattcgaatt tccaatcggc cgcggacgcg tcatcaaagg ttgggacgag
     1621 ggcgttgcca gtatgaaggt tggcggcaag cgcaccttgc tcatccctcc agaccttggt
     1681 tacggcgcgt ccggcgctgg cggcgtcatc ccgcccaacg caacccttct gttcgaagtc
     1741 gagttgctcg cgctgcgctg agctgagcgc cgcggctcac ccagcccatc cgaggcgcgc
     1801 ggcggttcca gtgctagcct ggtgacgcct gcacgaggac aagctgcgag gcacctgtcc
     1861 tgggacaagt cgtgaagccc gagccgactg tccagggaca agcagccgag gcaacctgtc
     1921 ctgggacaag tacgagggca cctgtcctgg gacaagtcct gagcccccgg acagcttggc
     1981 tcgtccaggg aacaagccgc cgaggcaacg tgtcctggga caagtcttga gccccgagcc
     2041 gactgtgcag ggacaagcag ccgagcacct gtcctgggac aagtcttgag cccccgagcg
     2101 gactgtccag ggacaagcag ccgaggcaac gtgtcctggg acaagtgcga gggcacctgt
     2161 cctgggacaa gtgcgagggc acctgtcctg ggacaagtct tgagccccga gccgactgtc
     2221 ctgggacagg cagccgaggc aacgtgtctg ggacaagtgc gagggcacct gtcctgggac
     2281 aagtgcttag agccggctgt cctgggacaa gcagcaggga gcagccatcg cagaagcccg
     2341 agggcccgcc tcccgcgcga ggaacgctgt cgcgcggagc tcgtcggagg ttgcgaatgc
     2401 cggggctggc aaccgaacgt tcgcgcgggc gatgtcagga atatgtcaga atttatccat
     2461 gcgtcgtaca gagcgcttgt ttgcgctgac cgagtacctg cgtgggcgcc gaagtggagt
     2521 aacggctgag gcgctagcgg agcggttcgg cgtgacgatc cgaacgatct accgcgactt
     2581 ggacgcgctt cgcgacgcgt ctcttccggt cgcggccgaa ccagggcggg gcgggggata
     2641 cgctctcgag aagagctaca gcttgccgcc cgtcaacttt acggcgcgcg aagcggcatt
     2701 gttggtggct ctcggccgct tcgcttcgga catgcgactt ttgccgttta cggagacgct
     2761 cgagtcggcg ctcgacaagg tgcgcgcagc gttatccgcc tcggcgcaac gagagcttct
     2821 gcagcgattg caagagctct ccttcatcgg tgtcccggcg ctgccagctg agaagtccgt
     2881 gcgcgccgcg atagaacgag cctggttcga acgacagccc ctggccatcg ttgcgctcga
     2941 gcagggctat atcgagacga aatgtcgtgt ccgcattcaa tctgtcgtga tggcgcgtca
     3001 cgacacgcga atacaaggcc tcgatctcga cgatggttcg cagcggaact tcaggttgga
     3061 ccgcatcctg aaggccgaag ttctcgagct cgactcgcac aagtagcgac cggactcgtg
     3121 agtttgtccc acgtaaaagt gctgcggccc ctttccgaag agatccgtac agcgcccggt
     3181 attcagtgtt cggacgtgag cgtgggatac accaagctca agcgatcaca gtcctcgttg
     3241 accccgagcc gacaattgtg gaccgagcga cagcgttcgg atggttcttc cgggtgcaag
     3301 caacccggcc ccgatactcg tagggctcgc gcggcggcgg gtaggtggcc gtgatggcac
     3361 ttgaaccgcg agagcgcgag cgaggcaccg cacacaacga atatgaacgc gagcggttcg
     3421 acgaagcgaa gcacctggtg gcgtgacatt tcgcgcgcga ctaggcagga tgcgtgccac
     3481 gccgtgttgc tgccaattgt gctcagtatt cgagcttcaa gaggcgggaa gcgccttcgg
     3541 ttgagaaggc agttgcgcaa tccagagcct gcttgcgcaa cgccgcgcag aagggccttg
     3601 aaatcaatga cgaacgtcac tcggtgatgt ttcgcgatcg ccaaatctgg tgaaggctgg
     3661 ggcaagagaa gcacggagta gcgattcgcg tttgaatcgg ttctcgtgat cgaagaacgt
     3721 cggcttcgat ctgaaggtgc caaccatcgc gacagccttt gggaggggac gagatgtcga
     3781 cgctcgaacg caagctttat cgcgatctct tccaattgaa ggcgcaaatc gcgagcatca
     3841 gcctggtatt tgcgagcggc atcgtgtgcc tcatcgcgat gcgaggttgc ttcatcgcca
     3901 tggagcgcgc tgtcgacgag ttttacgagc ggtaccggtt tgcgcacgtg ttcgcgcacg
     3961 cctcgcgtgt gcccgtctcg gttgcgccgc acatcgaggc gctcgcgggg attgccaccg
     4021 tcgaagctcg catcgctgaa gacgcctcgc taccgcttcc ggggctgcgc cagccagcgt
     4081 acggcaggat catttccttg cccgaaggtc gtcagccgag tgccaatgct ctgcatttga
     4141 agcgcggtcg cacgcccgcc tcgggccgtt ccgacgaggt cgtggtgctc gaggctttcg
     4201 cgaatgcaca cgggctcgag cccggggccg aagttccaat cgtcttgaac ggaaagcagc
     4261 ggtcgatgcg catcgtcggg atcgcgcttt cgccggagtt catctattcg attcgtccgg
     4321 gagcgctcgt cgatgatccg aagcggcacg ccgtgctgtg gatgacgcaa tcggcggtga
     4381 cggccgcatt cgggctcagc ggctgcttca acgatgtcgc cttgcggctc gaaccgggcg
     4441 cgtccgaagc cgcggtgcgc gccgcgctcg accgggtgct cgtaccttac ggaggcaccg
     4501 gcagcttttc gcggagggat caggtttcgc accgagccgt gacgcagcag cttgggcagt
     4561 tgcgcgtgct ttcgacgacg gtccccgtga tgtttctctc ggtggcggcg tttctggtca
     4621 acgtcgtgct cggccgcttg attcgtctgc aacggtcagc ggtggctctc ttgaaggcgc
     4681 tcggctacac gaatcgcgag atagcgcgcc actacgctgg ctacgttttt tctatcgtcg
     4741 tgccggggag cgggctcggt ttggttggcg gtgcgctgct cgggcgccac atgctgaggc
     4801 tttacgccga atttttccgt ttgccgagcc tctccctgga atttcacgtt ccgccagcgc
     4861 tcgtcggcta tgcgctcgtc gcgagcagca ttgcggcgct cgctggcgct ttgggcgcag
     4921 tgcgcagtgc catactgctt ccgcccgcgg aggcaatgca gccggcaagt ccgccgcgat
     4981 actcgaggag cgtcttcgag cgactcggcc tcggcgtgct cgcggggacg agtggtctga
     5041 tggtgtttcg cgaaatcggg cggcgcccat tgcggacggt cttttcagcg ctggggatgg
     5101 cgggtgcggt gtcgttgctc gtgctctcga ggttcggctt cgattcgctc aacgcctatt
     5161 tcgagcggac cttccgtcgc gagcaacggc aagacatcgt ggtgtcgttc aggggacctg
     5221 ctccgccgcg agtggtcgat gaattggctc gcgtgcccgg cgtggtctcg gctgaaggag
     5281 tacgcgcggt gccgatccga atccgacacg agcaccgaaa tcgtgacact gtgctgctcg
     5341 ggatcgctcc cgggtcgacc ctgcgcgtgc tcgtgtcgcg gaacggcgct cgggtcccgg
     5401 ttccgcccga cggcatcatc gtcgagcaga cgctggcgag caggttgggg ttgcgcctcg
     5461 gtgatcgccc cgaactcgaa gtgcacgagg gagagcggca aacactcacg ccggtcatcg
     5521 ttggtttcat cgacgagacc gctggtcttg gcgtgtacgc gcaagcgccg ttcgttgcga
     5581 gcctcatcgg cgacgtggga gccgtcacgt cggcattgct gacggtcgac ccgctccaac
     5641 tcgaagaggt gtatcgacgc ctccaccgct ccccttggat cctcgatgtt tcggacacta
     5701 aggaagaaat gaaccagatg cgcgacatga acacggcgtt catcaacgtg tggaccgcgg
     5761 tctcggtggt gctcgctggc ggcatcgtct ttggcgtcgt ttacaacaac gcacgcattg
     5821 ggctcgcggc gcgcgcgaga gatctcgcga cgcttcgcgt gctcggcttc agtcgtcggc
     5881 aaatctccgg aatcttgctc ggctcggagg cgttcgaggt cgcgctcgcc cttcccgttg
     5941 ggctctggct cggcgctgcg ctcgcgcgtg ctttcatgga aaacattgac ccggaagcgc
     6001 tccgttggaa cgtcgtgatt tctccatcga ctcatttcgc cgcgaccaca ttggtgcttg
     6061 cgcttgcgct aggaagcgct ctctgggtcc gtcgacgagt ggactcgttg gtgctgacag
     6121 atgtcctcaa agcgcgcgaa tgagtcgccg ccccgggcgc agggcagtcg gtgctcgcgg
     6181 gagctcagtg cgctcagggc agatgagtcg gctcctcgct ggagctcagt gcgctcaggc
     6241 caggtgagtc ggctgctcgc ggggctcacg gcgctcaggg caggtgagct acccagccct
     6301 cgtcatgacc atcgtgccgg ccatcgccag caagcacctt tccatcgtcg gagatccccg
     6361 cgaccgaatt caacgtccac ccggccgcgt caggcgagtt cttgatcaat tctgcccaat
     6421 tggtcgagga tccgtcgtcg acgtaaattg aagcgtagat gagtcggggc ggaccaatca
     6481 acattgggta gtaccactgc tctacgtagc cagcttgtac gcgaccatcg gcgctgcatc
     6541 ccgatgggac accggccccg agcggcattc cacccccctc gcgccagaaa ccgggcgcgc
     6601 cgttcagtac ggtactcaga tcgaaaccga catacaccgc gccaacgatg gtcgagccgt
     6661 ccgagcagac gcgcgtcgcc tcg
//
LOCUS       DASTKS010001255         7912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_871678, whole genome shotgun sequence.
ACCESSION   DASTKS010001255 DASTKS010000000
VERSION     DASTKS010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871678"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4183
                     /locus_tag="VFQ35_26825"
     CDS             <1..4183
                     /locus_tag="VFQ35_26825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934357.1"
                     /translation="LAASDVEQLLEQLKASPADDDVPSKLESAASHDEFAERIVDAFL
                     SSADALSDEEALEKKKSLLFRAARVSSAHSALRSRAEDIYARIHALSPEDEVAETALI
                     ELRRKLGKLEEVVEMLLGRAERAKARTEKSRHFAEIGRLYLSERGDREQALVAFTQAF
                     TENPSDSSVVQEIERLASSNAEAWNDVLGACGASVEDEATAAEERTLILMQSGRWWLD
                     RAQRPDLALSCFQAVVASEPSHEAALEGMAQIFRKSLQWAELGQVLLRRADASPSPAE
                     ARSLRVEAAEILANELGDPKSARALLEEVVSADPAHAKALPALLRLHEDAGDYGALVA
                     LLERQLPSQKEPERIRSLIRIGEIQEAHLENKLEAARHFELALDLDRTSLEALRGLER
                     LHQKAGRFKELLENLDRQVELSATPRQKVRLFERIAAIQGQEFLDHEKAAEALEQVLK
                     LDPSHESAYTSLARHYRAVKRFEDLATSYARHAELVSDPSQRISLLLEQARTLADAVG
                     APERALRAYEAVLALEPTHPEALEAVAKLKESTGDAGAALTAIEALAAKASTPETRAE
                     QWLRAARLLEGRGDRDGAIEQYKRALDANPKDPTASRALRDAYTARGDAHAAVQLFER
                     EIEQTEGNLGKAALAGKLALLQHEKLHDGARAEEIARRALEWDPGNIDALCVMGDVAF
                     ESRRFVEASRHYEAVQSRVDKLPNPTRVLIRYVDSLAQTGSTEKALAAMDTLLRLSPD
                     NPGALERAAEVNFEHGSPSRAAELFAELIERFGAGLDHHSRARILERRGEALRRAGRF
                     DEALAPLEEACELDPSRVDALVALAKVHEAAGRWLDVIKVKSRRLDNANGDDRVELLL
                     EIGEIWSSRLSDRAQAAKNLVAALDERPDDRRVLTRLMQLYSEDKDWNKLVAVVLRLA
                     EFVDDPKQRVKYLHTAAIVTLRQIGDAERALEFYEQVLELEPNFEKALSELTELERGR
                     ENHVAVERLLKRRLEIATKSDDRPSMLAAFEELGRLYEEKLGWTDQAVDAFEAAQTLD
                     PDNAERRDHLAELYASDPTKYLDKAVTAEHEKLRHSPFRVESYRTLRRLYTETKQADA
                     AFCLCQALTVLNLAEPDEERFYKRMRSETAAPAQNPLSDDAWLFQVMHPDTDALLTSL
                     FALIEGAVIARRGQSIAELGYEPGHQVNLAENPAPICQSLFYAAGVLGIPVPPAYQND
                     NDSRGVSFLFAHEPSLVLGTAAMNPDVPLQAAAFIAAQALTYLRPGMYLRHLLASGTA
                     LKAWLFGAIKLTAPQFPVQADLEGAVNEALAALEAGIHGPVRDHLTRVVSKLLTSGTA
                     LDLKRWVRGVDMTADRAALIAAHDLEMALRVVRASSDDATSIPVEERVKELVLYSVSP
                     EYFEIRQKLGISIDS"
     gene            complement(4220..5257)
                     /gene="gspN"
                     /locus_tag="VFQ35_26830"
     CDS             complement(4220..5257)
                     /gene="gspN"
                     /locus_tag="VFQ35_26830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HET9934358.1"
                     /translation="MQLPKIPARWKKALRIAGYVNFYFDALLLFAYLTFPYERVRDRI
                     VDEFNARQTGPDALRLEIDSMGPYWFTGVQAKGIHLISQPKPVAVTDPNAPAPKPSEL
                     TIESAHGRIAFLPLLVGSMRIAFGATTLGGEISGETWESEGARRLELELEDLSLDKVS
                     LLSDAVGLPLAGNLGGTVEFMLPEGKVAKADGKVQLKIKGLTAGDGKAKIRDTIALPQ
                     VDAGELTLDAEATAGSLKVKTLSASGPDLDLSADGTIRLRDPATASLLSLNARFRFNE
                     RYMGKNDTTRALFGSPGSAVPGLFDLDPKTKRAKGPDGYYGWRVTGLLNQPTFVPHAG
                     AGGGQAGAPKP"
     gene            complement(5257..5856)
                     /locus_tag="VFQ35_26835"
     CDS             complement(5257..5856)
                     /locus_tag="VFQ35_26835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934359.1"
                     /translation="MTLRERLGRLEDRERRLLGILALVIGVILLLALPFGVTALLHGN
                     RSDNEALRDAIQQINASRAMADRSRALHRAIEQRYANPTPPLTSFLATVAKDVEVEIP
                     ESQDRPVVNHGKRYEEKSTKIMLRKVGMLKVVRLMERIEQSGYPVSISQLNIRKRGVE
                     PDAFDVDMVVSAFERKPEKAVAKPKGKEEGASEPAKETP"
     gene            complement(5853..7376)
                     /gene="pilM"
                     /locus_tag="VFQ35_26840"
     CDS             complement(5853..7376)
                     /gene="pilM"
                     /locus_tag="VFQ35_26840"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET9934360.1"
                     /translation="MARFIGLDIGAHYVRAAVITTAYRRITVERLEETAISEVGVGRD
                     EAVRAAVTTAASHLLAHIDGVATALEGDQILSHRFTLPPTAAKQLSEVLPFELEAQVP
                     IDVEELVFDYRVLARPSNQAPLTVLVAAARIEHVRSRIDLVRAALGREPDRVACGSMS
                     LANLASVSDALRAPGPIAVLDLGGRRTEITVMTSGQPVYVRTLSRGVEGLPESASGLA
                     AEIRQSLLKWSAQGGGDVQTIYLAGGGASAEGADRFLTHELDLPVLPLPTLQFSEMNP
                     DLVAVLPRFAKAVALGLGAAGRGHDVDLRRGPLAYQRGFGFLKEKAPLLVGLGSATLV
                     SFFFATWAESRALSRENTVLTEQLAVVSKEVLGAPVEDAEGAISAIEKLRAGDEPDPL
                     PQMDAFDVVVEISKAIPANITHDIEEFDMQRGHVKIQGIAGTASEAQTIATEIGKRRC
                     LEGAKLGRVSAAVNSDRQKYVLETDVRCPDEAKKKKKPDDKAEKTTEKPEDKAGEKQ"
     gene            complement(7380..>7912)
                     /locus_tag="VFQ35_26845"
     CDS             complement(7380..>7912)
                     /locus_tag="VFQ35_26845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934361.1"
                     /translation="LMLICSNAVPGTKLCTDPMEMQKFLTVVTLMRGFTMGAPPFGSP
                     GALVSLIQGKGFLAPLITAAGLEPVTLVSPDVAKKQMTTSSKVFSLYATGIVKAGKRE
                     SRVHIHVVVDFRSAPEPGSPTTGSGTQPGAAGTATSVAPQPQTGVQSAAAAQQDAIAA
                     LTRPNPAGNVVYFKMD"
BASE COUNT         1361 a         2694 c         2560 g         1297 t
ORIGIN      
        1 gctcgccgcg agcgacgtcg agcagctgct cgaacagttg aaggcctctc ccgctgacga
       61 cgacgtgccc tcgaagctcg agagcgcggc gtctcacgac gagttcgccg agcgcatcgt
      121 ggacgcattt ctaagctccg cggacgcgct ctcggacgag gaggcgctcg aaaaaaagaa
      181 gagcttgttg ttccgcgccg cgcgcgtctc gtcggcacac tccgcgctac gctctcgcgc
      241 ggaggacatc tacgcgcgca ttcacgcgct ttctcccgag gacgaggtcg cggagaccgc
      301 gctcatcgag ctccgacgaa agctcggcaa gctcgaagag gtcgtcgaaa tgctgctcgg
      361 tcgagccgag cgagccaaag cgcgcaccga aaagtcgcgc cacttcgccg aaatcgggcg
      421 gctctacctc agtgagcgag gcgatcgcga gcaagcgctc gtggccttca cccaagcatt
      481 caccgagaat ccgagcgact cgagcgtcgt ccaagagatc gaacggctag cgagttcgaa
      541 cgccgaagcc tggaacgacg tgctcggggc gtgcggcgcc agcgtcgaag acgaggcgac
      601 tgccgccgaa gagcgcacgc tcattctcat gcagagcggc cgctggtggc tcgaccgcgc
      661 gcagcgcccc gacctcgccc tctcttgttt tcaggcggtc gtcgcgagcg agccttcgca
      721 cgaagccgcg ctcgaaggca tggcgcagat ctttcggaag tcgctgcagt gggccgagct
      781 cggtcaggtc ctcttgcggc gcgccgacgc ctcgccgagc cctgccgagg cgcgcagcct
      841 acgcgtggaa gcggcggaga ttctggcgaa cgagctcggg gaccccaagt cggcgcgcgc
      901 gttgctcgag gaggtcgtga gcgccgatcc cgctcacgcc aaggccctcc ccgcgttgtt
      961 gcgcctccac gaagacgcgg gcgactacgg cgcgctcgtt gcgttgctgg agcgccagct
     1021 cccgagccaa aaggagcccg agcgcatccg ctctctcatc cgcatcggcg aaatccaaga
     1081 ggcgcacctc gagaacaagc tcgaggcggc ccgccacttc gagctcgccc tcgacctcga
     1141 tcggacttcg ctcgaagcgt tgcgcggcct cgagcggctc catcaaaaag caggtcgctt
     1201 caaggagctg ctcgagaact tggatcgcca ggtcgagctc agcgcgacgc cgcggcagaa
     1261 agtgcgtctc ttcgagcgca tcgcggcgat ccaagggcag gaattcctcg accacgagaa
     1321 agccgccgaa gcgctcgaac aggtgctcaa gctcgatccg tctcacgagt cggcctacac
     1381 gtcgctcgcg cggcactacc gcgcggtcaa gcgcttcgaa gacctcgcga cgtcctacgc
     1441 gcgacacgcg gagctcgtga gtgacccttc acaacggatc tcgctcttgc tcgagcaggc
     1501 ccgtaccctg gccgacgcgg tgggggcgcc cgaacgcgcg ctccgcgcct acgaggccgt
     1561 tctcgcgctc gaaccgacgc acccggaagc gctcgaggcg gtcgccaagc tcaaagagtc
     1621 gaccggcgac gcgggcgcgg cgctcactgc catcgaggcg ctggccgcga aggcatcgac
     1681 gccggaaacg cgcgccgagc agtggttgcg cgcggcgcgg ctgctcgagg ggcgcggaga
     1741 ccgcgacggc gcgatcgagc agtacaaacg cgccctcgac gcgaacccca aagatccgac
     1801 cgcgagccgg gcgctccgcg acgcctacac ggcacgaggc gacgcgcacg cggcggtgca
     1861 gctgttcgag cgtgaaatcg agcagaccga agggaacctc ggcaaggccg cgctcgcggg
     1921 aaagctcgcc ctgctgcaac acgaaaaatt gcacgacggc gctcgcgccg aagaaatcgc
     1981 gcgtcgcgcg ctcgagtggg accccggcaa catcgacgcg ctctgcgtca tgggcgacgt
     2041 ggctttcgag agccggcgtt tcgtcgaggc gagccgacac tacgaggcgg tccagagccg
     2101 ggtcgacaag ctgccgaacc ccacccgcgt cctcatccgc tacgtcgact cgctcgcgca
     2161 gaccggctcc accgagaagg cgctggccgc gatggacacg ctcctgcggc tctcgccgga
     2221 caaccccggg gcgctcgagc gcgccgcgga ggtcaacttc gagcacggct ccccctcgcg
     2281 cgccgcagag ctcttcgccg agctcatcga gcgcttcggc gcgggcctcg accaccactc
     2341 gcgagctcgc atcctcgaga ggcgcggcga agcgttgcgg cgcgccggtc gcttcgatga
     2401 ggcgctcgct ccgctcgaag aagcttgtga gctcgaccct tcacgagtcg acgcgctcgt
     2461 cgcactcgcc aaggtgcacg aagcagcggg acgctggctc gacgtcatca aggtgaagag
     2521 ccgccggctc gacaacgcca acggcgacga ccgagtcgag cttttgctcg aaatcgggga
     2581 gatttggtcg agccgcctct cggaccgagc ccaagccgcc aaaaacctcg tcgccgcgct
     2641 cgacgagcga cccgacgacc gccgcgtgct cacgcgcctg atgcagctct acagcgagga
     2701 caaggactgg aacaagctcg tcgcggtcgt gctgcggctc gccgagttcg tggacgatcc
     2761 gaagcagcgg gtgaagtacc tgcacaccgc cgccatcgtg acgctacggc aaatcggcga
     2821 cgccgagcgc gcgctcgagt tctacgagca ggtgctggag ctcgagccca acttcgaaaa
     2881 ggcgctgagc gagctcaccg agctcgagcg ggggcgagaa aaccacgtcg cggtcgaacg
     2941 tctcctcaag cgccgcctcg agatcgcgac gaagagcgac gatcgacctt cgatgctcgc
     3001 cgcgttcgaa gaactcggcc gtctctacga agagaagctc ggctggaccg accaagcggt
     3061 agacgccttc gaagctgccc agaccctcga ccccgacaac gcagagcgcc gcgatcacct
     3121 ggcagagctc tacgcttcgg atccgaccaa gtacctcgac aaggcggtca ccgccgaaca
     3181 cgagaaactg cgacacagcc cgttccgggt ggagtcgtac cgaacgttgc gacggctcta
     3241 taccgaaacc aagcaagcgg atgcggcctt ctgtctctgc caagcgctca ccgtcttgaa
     3301 cctggcggag ccggacgaag agcgcttcta caagcgtatg cgctccgaga cggcggcgcc
     3361 tgcgcagaac ccgctgagcg acgacgcctg gctgtttcag gtgatgcacc ccgacaccga
     3421 cgcgctcctc acgagcctgt tcgcgctcat cgaaggcgcg gtcatcgcgc ggcgcgggca
     3481 aagcattgcc gagctcggct atgaacccgg gcatcaagta aacctcgccg agaatccggc
     3541 gccgatttgc cagagcctgt tttacgcggc gggcgtgctc gggattccgg tgccgcccgc
     3601 ttatcaaaac gacaacgact cccgcggggt ctcgttcttg ttcgctcacg agccatcgct
     3661 cgtgctcggc accgccgcga tgaacccgga cgtgccgctc caggccgccg cgttcatcgc
     3721 agctcaggcg ctcacctact tgcgccccgg catgtacctg cgtcatctct tggcttcggg
     3781 gacggcgctc aaagcttggt tgtttggcgc catcaagctc accgccccac agttcccggt
     3841 gcaggcggat ctcgagggtg ccgtgaacga agcgctcgcc gcgctcgagg ccggcattca
     3901 cggccccgtg cgcgatcacc tcacgcgcgt cgtttcgaag ttgctcacct cgggcacggc
     3961 gctcgatttg aagcgctggg ttcgcggcgt cgacatgacg gcagatcggg ccgcgctcat
     4021 cgccgcgcac gatctcgaga tggcgctgcg tgtcgtccga gcgagcagcg atgacgcgac
     4081 gagcatcccc gtcgaagagc gcgtgaagga gctcgtgctc tactcggtga gccccgaata
     4141 cttcgagatc cggcaaaagc tcgggatctc gatcgattct tgaagccgcg ccttttacgc
     4201 agagccccgg cgtcgagcgt tacggcttcg gcgccccggc ttgtccccca cccgcgcccg
     4261 cgtgcgggac gaacgtgggt tggttcaaga ggccagtaac gcgccaaccg taatagccat
     4321 ccgggccctt ggcgcgcttc gtcttcgggt cgaggtcgaa gagccccggg accgccgaac
     4381 ccgggctccc gaagagagca cgggtggtat cgttcttccc catgtagcgc tcgttgaagc
     4441 ggaaacgcgc gttcaagctg agcaggctcg ctgtcgccgg gtcgcgcaag cgaatggttc
     4501 cgtccgcgct cagatccaga tcggggcccg aagcgctcag ggtcttcacc ttcaggctcc
     4561 ctgcggtcgc ctccgcgtcg agcgtgagct caccggcgtc gacctgcggc agcgcgatcg
     4621 tatcgcgaat ctttgctttg ccgtcgcccg cagtaagtcc tttgatcttc agctgcacct
     4681 tgccgtcggc cttcgcaacc ttcccttcgg gcagcatgaa ctcgacggtg ccgcccaggt
     4741 tacccgcgag cggcagcccc accgcgtccg acagcagact gaccttgtcg agcgacagat
     4801 cttcgagctc gagctcgaga cggcgcgcgc cttcgctctc ccaggtctcg cccgaaatct
     4861 caccgccgag ggtagtcgcg ccgaacgcga tccgcatgct cccaaccagc aacggtaaga
     4921 aggcgatgcg cccgtgcgcc gattcgatgg tgagctccga cggcttcggc gccggtgcgt
     4981 tggggtcggt gacggcgacg ggcttcggct gagatatgag gtgaattccc ttcgcttgca
     5041 cgcccgtgaa ccagtacggt cccatcgaat cgatctcgag tcgcagcgcg tcggggccgg
     5101 tttggcgcgc gttgaactcg tcgacgatgc ggtcgcgaac gcgctcgtac gggaacgtca
     5161 agtaagcgaa gagcagcagg gcgtcgaagt agaagttcac gtacccggcg atgcgcagcg
     5221 ccttcttcca acgcgcgggg atcttcggaa gctgcatcac ggggtctcct tggcgggctc
     5281 ggaggcgccc tcttccttgc ccttcggctt cgcgactgcc ttctccggct tgcgctcgaa
     5341 ggcgctcacg accatgtcga cgtcgaacgc gtcgggctcc acgccgcgct ttcggatgtt
     5401 gagctgcgaa atactcacgg ggtaccccga ctgctcgatg cgctccatca accgaaccac
     5461 tttgagcatg cccaccttgc gcagcatgat cttggtggac ttttcctcgt agcgcttgcc
     5521 gtgattgacg accggccgat cctgcgactc cgggatttcg acctccacgt ccttcgcgac
     5581 cgtcgccagg aacgaagtca acggaggtgt cgggttcgcg tagcgttgct cgatggcgcg
     5641 gtgcagcgcg cggctccggt cggccatggc gcgcgaagcg ttgatctgtt gaatcgcgtc
     5701 gcgcagagcc tcgttgtcgc tgcggttacc gtgcaggagc gcggtgactc cgaacggcaa
     5761 cgcgagcagc aaaatcacgc cgatgaccag ggcgagaatg ccgagcagcc tgcgttcgcg
     5821 gtcctcgagg cgccccaaac gttcgcgcaa ggtcattgct tctctcccgc cttgtcttcg
     5881 ggcttctccg tcgtcttttc ggctttgtcg tcaggcttct tcttcttctt ggcctcgtcg
     5941 gggcatcgca cgtcggtttc gagcacgtac ttttgccggt cgctgttcac cgccgcgctc
     6001 acccggccga gcttggcgcc ttcgaggcag cgccgcttcc cgatctcggt cgcaatcgtc
     6061 tgcgcttcgc tcgcggtgcc ggcgattccc tgaatcttca cgtggccgcg ctgcatgtcg
     6121 aactcttcga tgtcgtgcgt gatgttggcc gggatcgcct tggagatctc cacgacgacg
     6181 tcgaaagcat ccatttgcgg cagcgggtct ggctcgtcgc cggcgcgcag cttctcgatc
     6241 gcagagatgg ctccctcggc gtcttcgacg ggggcgccaa gcacctcttt gctgacgacc
     6301 gcgagctgct cggtgagcac ggtgttctct cgagatagag cccgcgactc cgcccaagtc
     6361 gcgaaaaaga agctcaccaa ggtggcgctc cccaacccga ccagcagcgg agccttctct
     6421 ttgagaaagc cgaagccgcg ttggtacgcg agcggcccgc gccgaagatc gacgtcgtgg
     6481 ccccgcccgg ctgcgccgag cccgagcgcg accgccttgg cgaagcgcgg cagcacggcg
     6541 acgagatccg gattcatctc cgagaactgg agcgtcggga gcggcagcac gggaaggtcg
     6601 agctcgtgcg tgagaaagcg atcggcccct tcggctgacg caccgccgcc ggcgagatag
     6661 atggtctgga cgtcgcctcc cccttgcgcc gaccacttga ggagcgactg ccggatttcg
     6721 gcggcgaggc cgctcgcgct ttccggaaga ccctcgacgc cgcgagagag ggtgcgcacg
     6781 tacaccggtt ggcccgacgt catcacggtg atttcggtgc gtctaccgcc caaatcgagc
     6841 accgcaatgg gccctggcgc gcgtagcgcg tcggatacgc tcgccaggtt cgcgagagac
     6901 atcgagccgc acgcgacgcg gtccggttcg cgcccgagcg cagcgcgcac gaggtcgatg
     6961 cgagagcgta cgtgctcgat gcgagccgcg gccacgagca cggtgagcgg cgcctggtta
     7021 ctgggacgcg ctagcacgcg atagtcgaag accaactcct cgacgtcgat cggcacctgg
     7081 gcctcgagct cgaacggcaa cacctccgag agctgcttgg cggcagtggg cggcagcgtg
     7141 aaccggtgcg aaaggatctg atcgccttcc agcgccgtcg cgacgccgtc gatgtgcgcg
     7201 aggaggtggc tcgccgcggt cgtgacggcc gcgcgtacgg cttcgtcgcg gccaacgcca
     7261 acttcggaga tggccgtctc ctccaagcgt tcgacggtga tgcgccgata agccgtcgtg
     7321 atcaccgcgg ctcgcacgta atgcgcgcca atgtcgagtc ctatgaatcg agccatctgt
     7381 cagtccatct tgaagtagac cacgttgccc gccgggttgg ggcgggtcag cgcggcaatc
     7441 gcgtcttgtt gcgccgccgc ggccgattga accccggttt gcggctgggg cgcgaccgac
     7501 gtcgcggttc cggccgctcc gggttgcgtg ccgctgccgg tggtcggcga acccggctcg
     7561 ggagcggacc ggaagtcgac gaccacgtgg atgtgcactc gcgactcccg cttcccggcc
     7621 ttcacgatcc cggtcgcgta aagcgagaac actttgctcg aggtggtcat ctgcttcttc
     7681 gccacgtccg gcgaaaccag agtcaccggt tcgagcccag ccgccgtgat gaggggcgcg
     7741 aggaagcctt tgccctggat gaggctcacg agagcgcccg gcgagccgaa gggtggagcg
     7801 cccatcgtga agcccctcat caacgtgacg acggtgagga acttctgcat ctccatcgga
     7861 tccgtgcaga gcttggtccc gggaacggcg ttgctgcaga tcagcatcag ga
//
LOCUS       DASTKS010001256         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_648469, whole genome shotgun sequence.
ACCESSION   DASTKS010001256 DASTKS010000000
VERSION     DASTKS010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648469"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..2053)
                     /locus_tag="VFQ35_26850"
     CDS             complement(38..2053)
                     /locus_tag="VFQ35_26850"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934362.1"
                     /translation="MTLNLARAAGFALLFASCHPAPRFPWASPSRSAPFEHADVRTMN
                     DDYRRTDAVVLLREVLVPTRAALDYAAYHANFIIADPAPEGEGTPGALPAFDQPNPFG
                     GALEKSSLTADVYATPLSESERTHALSFAPVLEFLRAGKVDGAQELERALAQVRDESW
                     GLARRPGVSNQSISEAFVHGTELWVKVEFQPWLKAAWSLPDQDGDGVPELYGKLSPSV
                     VNAAAIASIEGDYRKRRLSSEEVATWANRLASYWYPSFNTDLVPHEAVWPDSHTEAEV
                     KKRLGDRSFPNPTIVLRGKPEGVATYEVFFVKGLGGGSDSTPARPSAALPRLAKTTPT
                     PEPSATRVTIERELKESGGSFEQWTQATAPAAEIFRRRLRSLRGQVKALEGNDGFLFF
                     KNSLAYATAPDLEKQKKGKNPLPVIVEFQKTLAAHGVDFLFVPVPTKVEIYPDRLDAQ
                     LSPFAGRVVNPQGRKFLLALANAGVEVVDLLPAFLAARRERPNELIFQRQDTHWNDAG
                     LRLAANVISERLKKYPWYKELARHAQQFTTREAQFQRLGDLHSRLPNTAKSRYEPEQL
                     VAQQVIASDGSAYVDDPDSPIVVLGDSFTGVYELMDAEHAGLSAHIAKQVKYPVDLVM
                     SYGGGPNVRNKLMRRGAEALESKKLVVWVMTARDLYDYWEDWEPLGK"
     gene            complement(2070..>2546)
                     /locus_tag="VFQ35_26855"
     CDS             complement(2070..>2546)
                     /locus_tag="VFQ35_26855"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9934363.1"
                     /translation="RTKDADVVACVHADGQYSPEALPGLLAELSARRLDILQGSRIAS
                     GTALSGGMPLYKYVANAALNRLENFTLGLGMTDYHSGYLLYSRRALETVPFEQLSKGF
                     DFDLEVIASARARGLSVAEAPVPTHYGDEVSYLNPITYGLRVLRVLWRYRQGVYDP"
BASE COUNT          433 a          842 c          822 g          449 t
ORIGIN      
        1 ccagccaggc atacgagctg cagcagagct cgacgcttca cttaccgagc ggctcccaat
       61 cttcccaata atcgtagaga tcgcgcgcgg tcatcaccca cacgaccaac ttcttgctct
      121 cgagcgcctc ggcgccgcgg cgcatcagct tattgcgcac gttgggtccg ccgccgtagc
      181 tcatcaccaa gtccaccgga tacttcacct gcttggcgat gtgagccgag agcccggcgt
      241 gctctgcgtc catgagctcg tagacccccg tgaagctgtc accgagcacc acgatgggac
      301 tatcgggatc gtcgacgtac gcggaaccgt cgctcgcgat gacctgctgc gcaaccagct
      361 gctcgggctc gtagcggctc ttggccgtgt tcggcaagcg cgagtgcagg tccccaaggc
      421 gctggaactg cgcctcgcga gtcgtgaact gctgcgcgtg ccgggcgagc tccttgtacc
      481 aagggtactt cttcagtcgc tcgctgatca cgtttgccgc gagtcgcagc cccgcgtcgt
      541 tccagtgtgt gtcttgccgc tgaaagatga gctcgttcgg ccgctcgcgc cgcgccgcga
      601 ggaaagccgg cagcaggtcg accacctcga cgccggcgtt ggcgagcgcg aggaggaact
      661 ttcgcccctg cgggttgacg acacggcccg cgaaaggcga aagctgcgcg tcgagacgat
      721 ccggataaat ctccaccttg gtgggcaccg gcacgaacaa gaagtcgacc ccgtgcgcgg
      781 ccaaggtctt ttggaattcg acgatgaccg gcagcgggtt cttgcccttc ttctgcttct
      841 cgagatccgg ggcggtcgca taggcgaggc tgttcttgaa gaagagaaag ccgtcgtttc
      901 cctcgagtgc tttcacttgg cctcgaagcg agcgcagtcg gcggcggaag atctcggccg
      961 ccggcgccgt cgcctgggtc cactgctcga acgacccgcc gctttctttg agctcgcgct
     1021 cgatggtgac gcgtgtagcg ctcggctccg gcgtcggggt ggtcttggcg agccgcggga
     1081 gtgcagcgct cgggcgcgcg ggcgtcgagt cgctcccacc gcccaagccc ttcacgaaga
     1141 acacttcgta cgtcgcgacg ccctcgggct ttccgcgcaa gacgatggtg ggattcggga
     1201 agctgcggtc gcccaggcgc ttcttgacct cggcttcggt gtgagaatcg ggccacaccg
     1261 cttcgtgggg gacgagatcc gtattgaaag acgggtacca gtacgaagcg agccgattgg
     1321 cccaagtcgc gacctcttca gatgacagtc ggcgcttgcg gtagtcaccc tcgatactcg
     1381 cgatcgcggc cgcgttgacc acggaggggc tcagcttgcc gtagagctcg ggcacgccgt
     1441 ccccgtcttg atccggcaga gaccaagccg ccttgagcca cggctgaaac tcgaccttca
     1501 cccagagctc ggtgccgtgc acgaacgctt cactgatgct ctggtttgac actcccggtc
     1561 gccgcgcgag cccccacgac tcgtcgcgaa cctgcgcgag agcacgctcg agctcctggg
     1621 caccgtccac cttgcccgcg cgtagaaact cgagcacggg cgcgaacgac agcgcgtggg
     1681 ttcgctcgct ttcgctcagc ggggtcgcgt agacatccgc ggtcaatgag ctcttctcga
     1741 gcgcgccgcc aaaaggattg ggctggtcga aggcgggcag cgcgcccggc gtcccctcac
     1801 cctccggcgc gggatccgcg attatgaagt ttgcgtgata ggcggcatag tcgagcgccg
     1861 cccgagtcgg caccagcacc tcacgcaaca gcacgacggc gtccgtgcgc cggtagtcgt
     1921 cgttcatcgt tcgcacgtcg gcgtgttcga acggtgcgct ccggctcggt gacgcccagg
     1981 ggaatcgggg cgccgggtga cagctcgcga acagcagagc aaagcccgcc gcgcgcgcca
     2041 aattcagcgt catctttcga ttctcctcgt caggggtcgt agacaccttg tcgataccgc
     2101 cagagcacac gcaagaccct gagcccgtac gtgatcggat tcaggtagga tacttcgtcg
     2161 ccgtaatgag tgggcacggg cgcttccgcc accgaaagcc cgcgcgcccg cgcgctcgcg
     2221 atgacttcga gatcgaagtc gaagcccttc gacagctgct cgaacgggac cgtctcgagc
     2281 gcccgacgcg aatagaggag atagccgctg tgataatcgg tcataccgag acccagcgtg
     2341 aagttctcga ggcgattcag cgcggcgttt gccacgtact tgtagagcgg catgccgccg
     2401 gagagcgcgg tgccagaggc gatgcgcgac ccctgcaaga tgtcgagccg gcgggcgctg
     2461 agctcagcga gcagccccgg cagagcctcg gggctgtact ggccgtccgc gtggacgcag
     2521 gcgaccacgt cggcgtcctt cgttcg
//
LOCUS       DASTKS010001257         5232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1096226, whole genome shotgun sequence.
ACCESSION   DASTKS010001257 DASTKS010000000
VERSION     DASTKS010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096226"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3343)
                     /gene="tssM"
                     /locus_tag="VFQ35_26860"
     CDS             complement(<1..3343)
                     /gene="tssM"
                     /locus_tag="VFQ35_26860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HET9934364.1"
                     /translation="MLWVIILSVLLLAATWLVVLLVKLPIWIAIVVTVVILLGIATFF
                     VVRMIRAQMKAAALERELLRQASAQAERVRPDKRPEILALQAQMKASLDTLKRSKLGK
                     GGKSALYTLPWYAIVGPPAAGKTTALSQSGLGFITPAGAGGAKVRGTAGTKNCDWWFS
                     QHAILLDTAGRLSTEEDDREEWTTFLDTLRRFRPERPLDGIVVALSVEDLLSGNEEQN
                     EELVKKLRQRVDELMGKTEMVLPVYVMFTKVDLIAGFVEFFGDLGKAQRGQAWGASFS
                     IDDERLDDPARATESEFDLLMKVLHAHMLERLAREPQPDVRQRVMQFPLEFQALAAPA
                     ARFVEELGRPNPYQETPLVRGFYFSSGTQTGRAMDRVLANMARGFDLRGFQGAEQRPT
                     QAQSFFVTDLFAKVIFPDRHLAVRSMSRIKRRNRQRAIWAAAAVLITMLIVAPATSAY
                     VDNSDLMQATERDVQQARSLERSSEASGAANALNLLLDRTEQLEREQSKTSIRGFFGP
                     YTAGELASSLRTIYIERLRAMVEGPVKSQLVSDVRAVGDLMRTDIKNFQSAYEDLKLY
                     VMLTQPQHLDKEFAAKRLSTVWARSLHSDSEADQQRLQAHAARYVDAFSLDPLWVWKA
                     DTAALSRAQGKLSMLPIDELRFSWLTEAAEGAPPIRPEKIFFGASAPYWKTRGNVEVP
                     GLYTALGWEKVRPLLESPDARLELEPWVLGQKSMIDLNDSRQNAAERLLDLYFQRYVA
                     AWSDFLAGLDVEAPKDMPAALMELRALAEADGPYVRLFRALSENVRLDISPSTLLGKA
                     MKKGEEIADKAIDKLKGVDAGTPPREISSVERQFQPLLKFAFGDSPNGKPDGAPTGLA
                     QYLVQLNTLYVSLSQLAESRAEPSADFAAELSRTSTAVQILLGGTDPRTRLILEPLLM
                     NPIRGSRAGVVRADFASLSEHWKAEVWENYNTKIQTRFPFANTQDEVTVPEFVEFFRP
                     ESGILWKFFKANLEGRLDRVGDGFVPKPSADPMPFRADFLSCLGVAGQITESIFGSAP
                     EPNLQFAIRIHPVGAEVAEIAFVVDGQATVYRNEPERWAPVQWPGKGDPHGGTLVVKG
                     ASFTDQIPRLGDFGLFRLLASGGIKP"
     gene            complement(3352..4029)
                     /gene="icmH"
                     /locus_tag="VFQ35_26865"
     CDS             complement(3352..4029)
                     /gene="icmH"
                     /locus_tag="VFQ35_26865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HET9934365.1"
                     /translation="MNEAMFWACADVLILGSQLPRAANLPPPAELRQRILTALDAMVG
                     KGRELGLPDIELVEARYALVAFIDEQILKSNWPGRAEWMGQPLQLQLYGEYTAGENFF
                     NRLRMLLQQGGTALALEVYYLCLALGFRGTYGNSADQRPLMQFSDAARQRVTKGLPSA
                     SKLGPHAEPHERVGAARSSNAPLIGLVVGCIIVALLVVFGLERILHMHLKDTLQAMPA
                     GTTRAGS"
     gene            complement(4108..>5232)
                     /gene="tssK"
                     /locus_tag="VFQ35_26870"
     CDS             complement(4108..>5232)
                     /gene="tssK"
                     /locus_tag="VFQ35_26870"
                     /inference="COORDINATES: protein motif:HMM:NF017723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HET9934366.1"
                     /translation="GEGTECPAPAPRALPADGAQVEVFLGLAHEMDATALVGSEDDAA
                     VRRFLRATKGVVDLNSGTSPQEVEWARPNVRVFFGGERHEGFAVIRVAVLLRQENGQF
                     KVLDTYIPPVLYVQAAPFLATGVRGILAQVVARQQQLAGERRQRQSGSVDFHSTEVRK
                     FWMLHTLNGAIPLLQHLLDTPRTHPEEAYAALAQLAGQLCSFSADVEPTAVPKFNYLE
                     LGDVFETLFAVVRRLLPGSIERAYTEIGLEHRPDGMFIGKFPDRGLLTQELFVAVAAN
                     MTEALVRERVPGVLKLASWNQVYDIVKQARHGVRVEIEWNPSAALPVKPGLCFFRLRK
                     EGPFWDDIAKSSTVALYLPADGEWTGANISLYAVPAANLR"
BASE COUNT          912 a         1705 c         1748 g          867 t
ORIGIN      
        1 ttggcttgat gccgccggaa gcgagcaatc ggaagagccc gaagtcgccg aggcggggga
       61 tttggtccgt gaagcttgcg cccttcacca cgagcgtgcc gccgtgcggg tcgccttttc
      121 cgggccattg gacaggggcc caacgctcgg gctcgttgcg atacaccgtc gcctgcccgt
      181 cgactacgaa ggcaatctcg gcgacctcgg cgccgacggg atgaatccgg atcgcaaact
      241 gcaggttcgg ctcgggcgcg ctgccgaaga tcgattcggt gatttgaccg gcgacaccga
      301 ggcagctcag aaaatctgcg cggaacggca tcgggtcggc cgaaggcttc ggtacgaagc
      361 cgtcgccgac gcgatcgagg cgaccctcga gattcgcctt gaagaacttc cacaagatgc
      421 cgctctccgg gcggaagaac tcgacgaact cgggaaccgt gacctcgtcc tgcgtgttcg
      481 cgaaggggaa ccgggtctgg atcttggtgt tgtagttctc ccagacctca gccttccagt
      541 gttcgctgag cgacgcgaag tcggcgcgga cgacgccggc tcggctgccg cgaatcgggt
      601 tcatcaagag cggctcgagg atgaggcgcg tgcgaggatc cgtgccgccg aggagaattt
      661 ggaccgcggt cgaagtgcga gagagctccg ctgcaaaatc ggcgctgggc tccgctcgcg
      721 attcagcgag ttgactcagc gagacgtaca gcgtgttcag ctgaacgagg tactgcgcga
      781 gcccggttgg ggcgccgtcg ggctttccgt tcggggaatc accgaacgcg aatttcagca
      841 gcggttggaa ctgacgctcg acgctcgaaa tctcacgcgg gggagtgccc gcgtcgacgc
      901 ccttgagctt gtcgatggcc ttgtcggcga tctcctcgcc cttcttcatg gccttgccga
      961 gcagggtcga gggcgagatg tcgagccgga cgttctcgct gagcgcgcga aagagccgca
     1021 cgtaggggcc gtccgcctcg gcgagagccc tgagctccat gagcgctgcc ggcatgtcct
     1081 tcggcgcttc gacgtcgagc cccgccaaaa agtcggacca cgccgccacg tagcgctgga
     1141 agtagagatc gaggaggcgc tcggcggcat tttgccgcga gtcgttcaag tcgatcatcg
     1201 acttttgccc gagcacccag ggctcgagct cgaggcgagc gtcgggcgac tcgaggagcg
     1261 ggcgcacttt ctcccaaccg agcgcggtgt atagccccgg cacctcgacg ttgccgcgcg
     1321 tcttccaata cggagccgac gcgccgaaga agatcttttc ggggcgaatc gggggggcgc
     1381 cctcggcggc ttcggtgagc caggagaacc gtagctcgtc gatgggaagc atcgagagct
     1441 tgccctgcgc gcgagacagc gccgccgtgt ccgctttcca aacccagagc ggatccagcg
     1501 agaaggcgtc gacgtagcgc gcggcgtggg cttgcaaacg ctgctgatct gcctccgagt
     1561 ccgagtgcag cgagcgcgcc cacaccgtcg agagccgctt cgccgcgaac tctttgtcga
     1621 gatgctgcgg ctgcgtgagc atcacgtaaa gtttcaggtc ctcgtacgcg ctctggaagt
     1681 tcttgatgtc ggtgcgcatc aaatcgccga ccgcgcggac gtcggagacg agctgcgact
     1741 tgaccggacc ctccaccatc gcgcggagcc gctcgatgta aatcgtgcga agagacgacg
     1801 cgagctcgcc tgccgtatac ggcccgaaga agccacggat cgacgtcttc gactgctccc
     1861 gctcgagctg ctcggtgcga tcgaggagca ggttgagcgc gttcgcggcg ccgctcgctt
     1921 cgctgctgcg ctcgagcgac cgggcttgct ggacgtcgcg ctccgtcgcc tgcatcagat
     1981 ccgagttgtc gacgtaggcg gaagtcgcgg gcgccacgat gagcatcgtg atgagcaccg
     2041 ccgcggccgc ccagatcgcg cgctggcggt tgcggcgttt gatgcggctc atcgagcgca
     2101 cggcgaggtg ccgatccgga aagatgacct tcgcgaacag atcggtgacg aagaagctct
     2161 gggcttgcgt cggccgttgt tccgcgccct ggaaaccgcg caggtcgaag ccgcgcgcca
     2221 tgttggcgag cacgcgatcc atcgcgcgtc ccgtctgcgt gccgctcgag aagtagaagc
     2281 cgcgaaccag cggtgtctct tggtacggat tcgggcgtcc gagctcttcg acgaagcgcg
     2341 ccgcgggcgc ggcgagcgcc tggaactcga gcgggaactg catcacgcgc tgacgcacgt
     2401 ccggctgcgg ctcccgcgcg aggcgttcca gcatgtgcgc atggagcacc ttcatgagga
     2461 gatcgaactc gctctcggtg gcgcgcgccg gatcgtcgag ccgctcgtcg tcgatgctga
     2521 agcttgcccc ccacgcctgc cctcgctgcg ccttgccgag atctccgaag aactcgacga
     2581 agcccgcgat gaggtccacc ttcgtgaaca tcacgtacac ggggaggacc atctccgtct
     2641 tgcccatgag ctcgtcgacg cgttgacgca gcttcttcac gagctcttcg ttttgttctt
     2701 cgttgccgct cagtaagtcc tcgacgctga gcgcgacgac gattccgtcg agggggcgct
     2761 caggccggaa gcggcgcagc gtgtcgagaa aagtggtcca ctcttcgcgg tcgtcttcct
     2821 cggtcgagag ccgcccggcc gtgtcgagca ggatcgcgtg ctgtgaaaac caccaatcgc
     2881 agttcttggt accggccgta ccgcgaacct tcgcgccgcc cgcacccgca ggagtgatga
     2941 agccgagccc cgactggctg agcgcggtcg tcttcccggc cgcggggggc ccgacgatgg
     3001 cataccaggg cagcgtatac agcgccgatt tgcctccctt gcccagcttg ctgcgcttca
     3061 acgtgtcgag cgaagccttc atctgcgctt gcagcgcgag gatctcgggc cgcttgtcgg
     3121 ggcgcacccg ctcggcctgg gcggaggctt gacgcaagag ttctcgctcg agcgcggccg
     3181 ccttcatttg cgcgcggatc atacgcacga cgaagaaggt ggcgatcccg agcaggatga
     3241 ccaccgtgac gacgatggcg atccaaatcg gcaatttgac gagcaagacg acgagccacg
     3301 tcgccgcgag cagaagcacc gagagaatga tcacccacaa catcggcgct cctacgaccc
     3361 cgctcgcgtg gtgccagccg gcatggcctg caaagtgtct ttcaggtgca tgtgcaagat
     3421 gcgctcgagc ccgaacacga cgagtagcgc gacgatgatg cagcccacga cgagcccgat
     3481 gaggggcgcg ttgctcgacc gagccgcgcc gacgcgttcg tgcggctcgg cgtgcggccc
     3541 gagcttcgac gccgaaggaa gccccttcgt gacgcgctga cgagccgcat cgctgaactg
     3601 catgagcggt cgctgatcgg cgctgttgcc gtaggtcccg cgaaacccga gcgcgaggca
     3661 gaggtaataa acttcgagcg cgagcgcggt gccgccttgc tgcagcagca tgcgcaagcg
     3721 attgaagaag ttctcgcccg ccgtgtactc gccgtagagc tggagctgga gcggctgccc
     3781 catccactcc gcgcgcccgg gccagttcga cttcaagatc tgctcgtcga tgaacgcgac
     3841 gagcgcataa cgggcctcca cgagctcgat gtcgggaaga ccgagctcgc gccccttgcc
     3901 gaccatcgcg tcgagcgcgg tcagaatccg ctgacgaagc tcagcgggcg gcggcaggtt
     3961 tgcggcgcgc ggcagctgcg agcccaagat gaggacgtcg gcgcaagccc agaacatcgc
     4021 ttcgttcacg cggcggtcct ccgtccacgt tctcggtcag ggaaaacata cgcggacgag
     4081 cggaggggaa tagccatggg gcgcccctca cctcaagttc gctgccggaa cagcgtaaag
     4141 tgaaatgttc gcgcccgtcc actcgccgtc agccggcaga tacagcgcga cggtcgaaga
     4201 cttcgcgatg tcgtcccaga aagggccctc tttgcgaagc ctgaagaagc agagcccggg
     4261 cttcaccggc agcgccgcgg acgggttcca ttcgatttcg acgcgtacgc cgtgccgcgc
     4321 ttgcttcacg atgtcgtaga cctggttcca gctcgcgagc ttcagcacgc cgggcacgcg
     4381 ctcgcgcacg agtgcttcgg tcatgttggc ggcgacggcc acgaagagct cctgggtgag
     4441 gagcccgcga tccgggaact tgccgatgaa catgccgtcg gggcgatgct cgaggccgat
     4501 ctccgtgtac gcgcgctcga tgctgccggg cagcaagcgc cgcacgaccg cgaacagcgt
     4561 ctcgaaaacg tctccgagct cgaggtagtt gaatttcggc accgccgtcg gctcgacgtc
     4621 ggccgagaag ctgcagagct gtccggcgag ctgggcgagc gccgcatacg cctcctcggg
     4681 gtgcgtgcgg ggcgtgtcga gcaggtgctg caacagcggg atcgcgccgt tcagcgtgtg
     4741 cagcatccag aacttgcgga cctcggtcga gtgaaagtcc accgagcccg actgccgctg
     4801 acgtcgctca ccggcgagct gctgctgtcg cgcgacgacc tgcgccaaaa tccctcggac
     4861 gccagtcgcg aggaacggcg ccgcctgcac gtagagcacc gggggaatgt acgtgtcgag
     4921 caccttgaac tgcccgttct cttgccggag cagcaccgcc acgcgaatca ccgcgaagcc
     4981 ctcgtggcgc tcgccgccga agaacacgcg aacgttcggg cgcgcccact cgacctcctg
     5041 aggcgaagtg ccgctgttca gatcgacgac gcccttcgtg gcacgcagaa agcgccgaac
     5101 cgccgcgtcg tcttccgaac cgacgagagc ggtcgcgtcc atctcgtgag cgagcccgag
     5161 gaacacctcg acctgcgcgc catctgcagg cagcgcgcgc ggcgcgggcg cggggcactc
     5221 ggtcccctcg cc
//
LOCUS       DASTKS010001258         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_648681, whole genome shotgun sequence.
ACCESSION   DASTKS010001258 DASTKS010000000
VERSION     DASTKS010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648681"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VFQ35_26875"
     CDS             complement(<1..1390)
                     /locus_tag="VFQ35_26875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9934367.1"
                     /translation="MDKQLWLKSYPEGVPHDIDPGRYASVVDVFRRACGKFGKKPCFT
                     NMDVTLSFEEVERLSQHFAAYLSKDLGLEKGDRVGIQMPNVLQYPVALFGAMRAGLTV
                     VNTNPLYTPREMEHQYRDAGVKAVVTLENFASHLQEVWTNIDRPKVIITGIGDLFPAP
                     KRQIVNFVVRHVKRMVPAYEIPGMIGMREALARGARHSGFHDVPVGPDDLAFLQYTGG
                     TTGVAKAAMLTHRNVIANMEQASAWMGPRFVEGEEIVMSPLPLYHVFSLTVNCLLFLK
                     WGGQNVLVTNPRDFPAFIKLLQKQPFTVLTAVSTLLNALMNQPGFSAIDFSKVKLTVA
                     GAMALSRSVAERWRTLTKSRLIEGYGLTEASPIVSANPVNGGDRLGSIGLPFPSTEIR
                     LVDESGNDVGHGEAGELVVRGPQVMKGYWNRPEETERVLRDGWLFTGDIAEVDNDGFF
                     RIVDRKKDMILVS"
     gene            complement(1407..3068)
                     /locus_tag="VFQ35_26880"
     CDS             complement(1407..3068)
                     /locus_tag="VFQ35_26880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934368.1"
                     /translation="MTFRLCAASRAAALLLASTVLGCNLEEGLRKTPTVLSPDEVAAI
                     EGPGKRLLAGNFHGLTTRWGRLVMRKVDGPKNTLVSVDPQHESSCELENAERFAYAST
                     GWVAYSDAASNFTEIHFAAEDCTALPAVVSNASMPYDVTEDGEAVMVIGSALVAVDPV
                     TEQRRVLADPYTSVQQLGSRDQKGYVWLVKQGDQFIEFDWNWKELSRVGEKVGGLAFV
                     SSPPTLAFEDNGKLNRMSVDADGKLSISLIAEDGCRPRTASSTNFAYLSPCNEPTLHT
                     VSGRGTDVDWGALGVQPEQAVALPKGTSGIQLLFLRDIDPNVGTGSLWLLDAPGGEPQ
                     KLAEHADLGWVQAAKDDSIYALVDIENDLGKVVHFAKDGSTEELVTEVPRNGNNQFYY
                     GRQFWFFAHPQSDGTSELVRICALASCDGNAGAPAVQSIARGVPDWGFASALDSNHVA
                     VLHDVSKGRGTLSTDSGALVAEGVPQGGFAALYPIMKQGIAYLTHYDSERAVGTLEYW
                     NLELDARGTVAENVSEFMRADWPYMGIVYLVPSGDNAGLWYARGK"
     gene            3123..5048
                     /locus_tag="VFQ35_26885"
     CDS             3123..5048
                     /locus_tag="VFQ35_26885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9934369.1"
                     /translation="MSDNSRQRSALALAFAFGCFSFTSLAAAQAPPAAPLEVVVEGER
                     AAPTSAQVTREQARALPGTFGDPLRAIEAEPGVTPIVSGLPYFFVRGAPPANVGFFID
                     GVDVPLLYHAFFGPSVIHPGLIQDVSLYSGVAPVEYGHFAGPVVAATTRPASYEFNGE
                     ATVRAIDAGALVEAPFGPCEGVERPGCARGSALVGGRYSYTGLVLSLLSDTKLSYWDY
                     QARVGYRLSPRDEVRLFAFGAYDYFDAGDSGIEKVGGTTQFHRLDLRYEHAFSAATRV
                     GVAVTLGFDRTGDEKSLVRDRSLRGRLELKHSLSPRNTLKAGADVRADGFSLAVDERA
                     SGFHDFSQLFPARNEFLGGTYVMLDWHAAPGIRVVPGVRADYYRSQGQSAFGVDPRIS
                     AEFVLAPGLRVEHAIGLSHQPPNFVPAIPGAQVADLKGGLQEALVASAGVRYELFKDV
                     NAQATVFRSAFFNALDPIGGGRQFTVDAEVLQQRATISSVGFELKLVRPLTRRLGGFL
                     AYTLSHTREASNTWVSVAGFDRPHVLQGALAYDFGAGVRAAVRAVYYSGIPARVVGTG
                     RPLFDGDRRGDPYFRLDARFEKRFRLGEKGMLSLVAEVLNATSSTEVLRLDCGNICQV
                     GRAGPVVLPSVGAEAHW"
     gene            complement(5211..>5963)
                     /locus_tag="VFQ35_26890"
     CDS             complement(5211..>5963)
                     /locus_tag="VFQ35_26890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934370.1"
                     /translation="AAPAVAQTRGADDATTAPSSASPSDAPASSAASPPAPAVPPPAS
                     EAAPTPPCRLGVHAGTRDEDAATVGSLVCRELEGKAGTQGHHYRVDLDSLGSSVFLTL
                     TDEDQPSSAGVRRMRLASIEEAADAAPRLVSALLSGKSVGDTERVGNLTGVEAQAPLQ
                     KSGSLQVGVGVLGVYAPSVSTLAPGFELGLRYDTPSWVAHTSLRYAAHLDRNSQGENE
                     GLRVFDWGVGARYMLSKQDIAPFVGGGSTQRP"
BASE COUNT          979 a         1934 c         1925 g         1125 t
ORIGIN      
        1 ccgaaaccaa gatcatgtcc ttcttgcgat cgacgatgcg gaagaagcca tcgttgtcga
       61 cctcggcgat atccccggtg aagagccagc catcgcgcag gacgcgctcc gtttcttccg
      121 ggcggttcca gtaacctttc atcacttgcg ggccgcgcac gacgagctcg cccgcttcgc
      181 catgccccac gtcgttgccg ctctcgtcga ccaaccgaat ctcggtcgac ggaaacggca
      241 gcccgatgga gccgagccgg tctccgccgt tgaccgggtt ggcgctcacg atgggcgaag
      301 cttcggttag cccgtagcct tcgatgaggc ggctcttggt gagcgtgcgc cagcgctcgg
      361 cgacgctgcg agacagcgcc attgctcccg cgacggtgag cttcaccttc gagaaatcga
      421 tcgctgaaaa gccaggctgg ttcatcagcg cgttgagcag cgtgctcacg gcggtgagca
      481 ccgtgaacgg ctgcttttgc aggagcttga tgaatgccgg aaagtcgcgt gggttcgtca
      541 ccagcacgtt ctgcccgccc cacttcaaga agaggaggca gtttacggtg agagaaaaca
      601 cgtgatagag cggcagcggg ctcatcacga tctcttcgcc ttcgacgaag cgtggcccca
      661 tccaagcaga cgcctgctcc atgttggcga tcacgttgcg gtgggtcagc atcgcggcct
      721 tggcgacccc cgtcgtcccc cccgtgtact gcagaaacgc gaggtcgtct ggccccaccg
      781 gtacgtcgtg gaaaccgctg tggcgcgcgc cgcgtgccag cgcctcgcgc atcccgatca
      841 tgcccgggat ttcgtatgcc gggaccatgc gcttcacgtg gcgtacgacg aagttcacga
      901 tctgccgttt gggggcaggg aacagatcgc cgatgcccgt gatgatgact ttcggccgat
      961 cgatgttggt ccagacctct tgcaggtggg acgcgaagtt ctcgagggtg acgacggcct
     1021 tgacgccagc gtcgcggtat tgatgctcca tctcgcgcgg cgtgtagagc gggttcgtgt
     1081 tgacgacggt gagccccgcg cgcatcgcgc cgaagagcgc aaccgggtac tggagcacgt
     1141 tcggcatttg gatgccgacg cggtcgccct tctcgagccc gaggtcctta ctgaggtagg
     1201 ccgcgaagtg ctggctcagc cgttcgacct cttcgaacga cagcgtaacg tccatgttgg
     1261 tgaagcacgg cttcttcccg aacttgccgc aagcgcgtcg aaacacgtcg accaccgagg
     1321 cgtatcgccc ggggtcgata tcgtgaggga cgccttccgg gtagctcttg agccagagtt
     1381 gtttatccat caaaccgcat cattgcctac ttgccccgcg cataccagag ccctgcatta
     1441 tccccgctcg gcaccaggta gacgatgccc atgtatggcc aatcggcgcg catgaactcc
     1501 gagacgtttt cggccaccgt gccgcgcgcg tcgagctcga ggttccagta ctcgagcgtc
     1561 cctacggcgc gctccgaatc atagtgggtg aggtacgcga tcccctgctt catgatggga
     1621 tagagcgcgg cgaaaccgcc ttgcgggacc ccctcggcga ccagtgcgcc gctgtcggtc
     1681 gaaagagtgc cgcggccttt cgaaacgtcg tgcagcaccg caacgtggtt cgaatcgagc
     1741 gccgacgcga acccccaatc gggtacgccg cgcgcgatgc tttgaaccgc cggcgcccct
     1801 gcgttcccgt cgcagctcgc cagcgcacag atacgcacca gctccgaggt cccgtcgctt
     1861 tgggggtgag cgaagaacca gaactgccga ccgtagtaga attggttgtt gccgttgcga
     1921 ggaacttcgg tgacgagctc ttcggtgctc ccgtccttcg cgaagtggac gacctttccg
     1981 aggtcgtttt cgatgtcgac gagtgcgtaa atactgtcgt ccttcgcggc ctgcacccag
     2041 ccgaggtcgg cgtgctcggc gagcttctgc ggctccccgc cgggagcgtc cagcaaccag
     2101 agcgagccgg tgccgacgtt gggatcgatg tcgcgcagga acaacagttg aattccggaa
     2161 gtgcccttgg gcaaggccac cgcctgctcg ggctggacgc cgagcgcgcc ccaatcgacg
     2221 tccgtgcctc gccccgagac cgtgtgcagc gtgggctcgt tgcaggggct caggtaagcg
     2281 aagttggtcg agctcgcagt gcgcgggcgg cacccgtcct cggctatcag cgaaatcgag
     2341 agcttgccgt cggcgtcgac gctcattcga ttgagcttgc cgttgtcttc gaatgccaac
     2401 gtcggtggcg acgagacgaa cgccaagccg ccgacctttt cgccgactcg tgaaagttcc
     2461 ttccagttcc aatcgaactc gatgaactgg tcaccctgct tgacgagcca gacgtagccc
     2521 ttttggtcgc gagagccgag ctgttgaacg gaggtgtagg gatccgcgag gacgcgtctt
     2581 tgctccgtga ccggatcgac cgccacgagg gcgctgccga tcaccatcac ggcttcgccg
     2641 tcttcggtga cgtcgtacgg catggaagcg ttcgagacca ccgcgggtaa ggcggtgcag
     2701 tcctccgccg caaagtggat ctcggtgaag ttcgacgcgg cgtcggagta agccacccag
     2761 ccggtgctcg catacgcaaa gcgttcggcg ttttcgagct cgcacgagct ctcgtgttgc
     2821 ggatccaccg agacgagcgt gttcttcggc ccgtcgactt tgcgcattac gagccttccc
     2881 caacgggtcg tgagcccgtg gaagttgccg gccaggaggc gcttgccggg gccctcgatg
     2941 gcggcgactt cgtccggtga gagtaccgtt ggcgtctttc tcagaccctc ctcgaggtta
     3001 catccgagga ccgtcgatgc gagaagtaag gccgctgcac gagaagcggc gcagagtctg
     3061 aaagtcatgc tacaccccta aacatcgagg gaaccagcgc gtggcggagc ttggctcgat
     3121 tcatgagtga taactcgcgg cagcggagcg cgctcgcgct agcgtttgcc tttgggtgct
     3181 tttcgttcac ttcgctggcg gcggcccagg ccccaccggc tgctccgctc gaggtcgtcg
     3241 tcgaaggcga gcgcgccgca ccgacgagcg cgcaagtcac gcgcgagcag gcccgcgcac
     3301 tgcccggaac cttcggtgat ccgctgcgcg cgatcgaagc cgaacccggc gtgaccccca
     3361 tcgtatcagg cttgccgtac tttttcgtgc gcggcgcacc gcccgcgaac gtggggttct
     3421 tcatcgacgg cgtggacgtg ccgctactgt accacgcctt tttcggcccc tcggtcatcc
     3481 accctggtct aatccaagac gttagcctgt attccggggt cgcgcccgtc gaatacggtc
     3541 actttgcggg accggtggtt gctgcgacga cgcgtccggc gtcttacgaa ttcaacggcg
     3601 aggccacggt tcgagccatc gacgccggcg ctttggtcga ggcgccgttc ggtccgtgcg
     3661 aaggcgtcga gcgccccggc tgcgctcgcg gcagcgcgct agtcggtggg cgctactcgt
     3721 acacgggcct cgtgctctcg ctgctcagcg acaccaagct atcgtactgg gactaccagg
     3781 cgcgcgtcgg gtaccgcttg agcccgcgcg acgaagttcg gctcttcgcg ttcggggcgt
     3841 acgactattt cgacgccgga gacagcggca tcgagaaggt cggcggcacg acgcagtttc
     3901 atcgcctcga cttgcgttat gagcacgcct tctcggccgc gactcgcgtc ggtgttgcag
     3961 tgactttggg tttcgatcga actggcgacg agaagagctt ggtacgcgac cgctcgttgc
     4021 gggggcgcct cgagctcaag cactccttgt ctcctcgaaa taccctcaaa gccggcgccg
     4081 acgtacgcgc ggacggcttc tcgctcgcgg tggacgagag ggcgagcggc tttcatgatt
     4141 tttcgcagct cttcccggcg cgcaacgagt ttttgggtgg cacctacgtc atgctcgact
     4201 ggcatgccgc gccagggatt cgagtcgttc ccggcgtccg cgcagactac taccgctccc
     4261 agggccaatc cgcgttcggg gtggatccgc gcatcagcgc agagttcgtg ctggccccag
     4321 gcttgcgcgt agagcacgcg atcggtcttt ctcatcagcc acccaatttc gttcccgcga
     4381 tcccgggcgc gcaggtcgcc gacttgaagg gtggtctgca ggaggctctc gtcgcgagcg
     4441 cgggcgtccg ctacgagctc ttcaaagacg tgaacgcgca ggcgaccgtg ttccggtcgg
     4501 cgttcttcaa tgccctcgac ccgatcggcg gcggacgcca attcaccgtc gacgcagagg
     4561 tcctgcagca gcgcgcaacc atttcttcgg tcggcttcga gctcaagctg gtgcgcccgt
     4621 tgacgcgccg gctcggaggt ttcctcgctt acactttgtc gcacacgcgc gaggcgagta
     4681 atacgtgggt ttcggtggct ggcttcgatc gcccgcacgt gctccaaggc gcgctcgcct
     4741 acgacttcgg cgcgggcgtc cgcgccgcgg tgcgcgccgt atactactcc ggcattcccg
     4801 cgcgtgtggt cggcactgga cgtcccctat tcgacggtga ccgacgcgga gacccttatt
     4861 tccgcctgga tgcgcgcttc gaaaagcgat ttcggctggg ggagaaaggc atgctgtcgc
     4921 tcgtcgcgga agtgttgaac gcgacttcga gcaccgaggt gctccgtctg gactgcggca
     4981 acatatgtca ggttggtcgc gccggtccgg tcgtgcttcc cagcgtcggc gccgaagcgc
     5041 actggtagct tcaatcgaac gagagcgtga ccgcgagcga cagcggcatc acgtaacgct
     5101 gcgacaccga gaacatgggt gcgtccgccc tcagcgcggc gccgagatgg ttccggtgga
     5161 ggcgcagcat ctcgaccccg acctccccga atgcgccaag cccgttttcg ctatggtcgt
     5221 tgcgttgaac cgccgccgac gaaaggagcg atgtcctgct tgctcagcat gtagcgcgct
     5281 ccgacccccc aatcgaagac gcgcagccct tcgttttctc cctgcgagtt ccgatccagg
     5341 tgcgcggcgt aacggagcga ggtgtgcgcg acccagctcg gcgtatcgta tcgcaggccg
     5401 agctcgaacc cgggcgccaa cgtggaaacc gagggcgcat aaacgccgag gacgccgacg
     5461 cccacctgaa gcgagcccga tttctgaagc ggagcttgcg cctcgactcc ggtcaagttg
     5521 ccgacgcgct ccgtgtcgcc cacgctcttt ccggagagca gcgcgctcac gaggcgcggc
     5581 gccgcatccg ccgcttcttc gatcgaggcg agccgcatgc gacgcacgcc cgcgctgctg
     5641 ggctgatctt cgtcagtgag cgtgagaaag acactactgc cgagcgaatc caagtcgacg
     5701 cgatagtgat gaccttgcgt gcccgccttc ccctcgagct cccggcacac gagagagccg
     5761 acggtcgccg cgtcttcatc gcgagtgccg gcgtgcaccc caagccgaca ggggggtgtg
     5821 ggagcggctt cgctcgcggg cggtggcacc gcgggcgcgg gaggtgacgc agcggaactc
     5881 gcgggcgcgt cgctgggtga ggcgctactc ggagccgtgg ttgcgtcatc cgcacctctg
     5941 gtttgagcga ccgcgggtgc agc
//
LOCUS       DASTKS010001259         6236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_204088, whole genome shotgun sequence.
ACCESSION   DASTKS010001259 DASTKS010000000
VERSION     DASTKS010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204088"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFQ35_26895"
     CDS             complement(<1..724)
                     /locus_tag="VFQ35_26895"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9934371.1"
                     /translation="MNAGFGTEQLVRLRDLVTAGLGLAFADEQLEPLGAVATKRMHTL
                     NLATSHEYLARLEDPEARRAELAALAQFLTVPETFFFRGEEQFRVLIDASTAKVAEHG
                     RALRLLSAGCASGEEPYSLAIALREALRSIQDWNISIDAFDVNPSMIDAAKRGSYGRW
                     SLRATPDLLKQRYFRAEDRTFEVDRGVKAMVRFECKNLADPASLRAEGAYDAIFCRNV
                     LMYFDLEVARRIIRSFEKALKPT"
     gene            complement(721..1185)
                     /locus_tag="VFQ35_26900"
     CDS             complement(721..1185)
                     /locus_tag="VFQ35_26900"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9934372.1"
                     /translation="MSNRPGLRVLVIRCGDSACALPLVGLNEVMRPLALEPSPRAPPF
                     VLGMSMIRGTLVPVVDLALLVTGAPTARIGRYLTVSVGTRAVALAVSEVLGVHELARP
                     SFSSLPPLLGSEKSPLVQAIATLDSQLLRVLREARAVGQELFALIESQELSA"
     gene            complement(1208..3538)
                     /locus_tag="VFQ35_26905"
     CDS             complement(1208..3538)
                     /locus_tag="VFQ35_26905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934373.1"
                     /translation="MQARETVLLVDDEPQVLVALEDVLSEEYEIVKAGSAARALEIVA
                     HRPEIAVVLTDQRMPHMTGDELLARLAITTNMAHILVTGFAEPSAVIRAVNDGGIFAY
                     VAKPWNDDDLRLKVRRAVQHVRLARELMEERRLLQGLMDSVSDGIYFKDAALRFVRAN
                     RAFARSLGVAVPENLVGKSLKEIVPGAESEAAEREEALVLGGDEAAEDALRRAGSSEK
                     SRWFSEIRARLLNQSGEVVGIVGIARDVTERVRGDEALRQGEARYREQTALLNSILAG
                     IGEGVVVLDRSGHVVLFNRRAEKILGIGPPEVEPVGLAEAYGIYGRDQAPLASEENPL
                     LRAMAGEERTEAEVFVKNARVSGANVAVTATPLRDDYGNLRGSIALLHDVTAQRQLEK
                     KLSQSQKLEAIGRLAASVAHDFNNLLSVILSYSSLALNELPADASVRADVEAIRGAGE
                     RAAELTRQLLAFGRAQVPSVRVVDVNAIVRDSAKLLQRLLGDGIELRVHSQGSWCVRA
                     DPRQIEQVVMNLAVNARDAMPEGGELLIATSNCELSAAPRDEWPHAEPGRYVMLTVSD
                     TGVGMDSATIARIFEPFFTTKEFGKGTGLGLATVESIVKQSHGHLRVDSALGKGTRFG
                     IILPATEEAASSVNTLGPPMKLEGSETILLVEQQEEVRRAAAQVLRRRGYRVFEARGA
                     REALILCESSPEPIDLLLTDVASPPSGHQLPARLRALQPALRVLLMSGQMDAAATQPR
                     GGDGAQEYFLQKPFSAEGLLRRVREVFDDTLRHALN"
     gene            complement(3558..5597)
                     /locus_tag="VFQ35_26910"
     CDS             complement(3558..5597)
                     /locus_tag="VFQ35_26910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934374.1"
                     /translation="MTANILIVDDSLTVRMDLTEAFAKVGLRPLSCATLAEARKLLAE
                     QNVGVVILDVVLPDGDGVDLLRELRSSAGSETPVLMLSTESEISDRIRGLKTGANDYV
                     GKPYDRGYVIARARELLRRNSRASDGKTVVLIEDSLTARAELSQVLARAGYKVLTAAS
                     GEEGLRFLDEVRPVALIVDSVLPGMDGAAVIRRVRLDAALRRLPCLLLTGDGAVSEEL
                     RALDSGADAFVHKGDDPDIMLAKLKALLRSASDSSLESTTRSLLGPKQILAVDTEDGY
                     REAAANALREEGYDVISARSGEEAIDLLAVHSVDCILLNAELSGIDGRETCRRLKNSP
                     LIRDIPIVMSSAFDDRSTTLESLSAGADDYVEKSASFEILKARVRAQLRRRQVEDETR
                     RVREQLLRGELEAAEARAARELAATRAALVDELERKNRDLGSAYQALQETQAQLVQSA
                     KMASLGQLVAGVAHEINNPLSFALSHLKTARKSLDKVEAQVAHDDENWLRAKNRLSEI
                     DAGLERIRDLVVKLRTFSRLDEGERKRASIRECVESVLTILNHRLNENIRVETKFASR
                     DLVDCYPGPLNQAVMNLVTNAIDALGDEPGLIRIETEATDSELSIIVEDSGPGIEPGL
                     RERVLEPFFTTKPVGQGTGLGLSITYSIVEKHRGALVLTDREGGGTRAIIRIPIS"
     gene            5971..>6236
                     /locus_tag="VFQ35_26915"
     CDS             5971..>6236
                     /locus_tag="VFQ35_26915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934375.1"
                     /translation="MVLSLGKSLSVVAPLGYCLLAACGSDHQSQHANTPSGAVQEVSA
                     SAETRSATESISEARCVREQRCDNVGDNKKYSSMSDCMTRIRAD"
BASE COUNT         1076 a         2099 c         1924 g         1137 t
ORIGIN      
        1 ccgtcggctt gagcgctttc tcgaagcttc gaatgatgcg tcgagcaacc tcaaggtcga
       61 agtacatgag tacgttgcga cagaagattg cgtcgtacgc cccttctgct cggagcgagg
      121 ccgggtcggc gaggttcttg cactcgaagc gcaccatggc tttgaccccg cggtcgacct
      181 cgaacgtgcg gtcttccgcg cggaagtagc gctgcttcag caggtcggga gtcgcgcgga
      241 gcgaccatcg cccgtaggag cctctcttcg cggcatcgat catggaggga ttgacgtcga
      301 aggcgtcgat cgaaatgttc caatcctgga tcgagcgcag cgcttctcgc agcgcgatcg
      361 cgagcgagta cggctcctcc ccggacgcgc agcccgcaga gagtaggcga agcgcgcggc
      421 cgtgctccgc gaccttcgcg gtgctcgcgt cgatgagcac ccggaactgc tcttctccac
      481 gaaagaagaa ggtctcgggc acggtgagaa actgcgcgag cgccgcgagc tctgcccgac
      541 gagcctcggg gtcttcgagg cgtgcgaggt actcgtgcga cgtcgccaag ttcaacgtgt
      601 gcatgcgctt cgtcgcgacg gcgccgagcg gctcgagctg ttcatccgcg aacgcaagtc
      661 cgagcccggc cgtgaccagg tcgcggagcc gcacgagttg ctcggtcccg aaaccggcgt
      721 tcatgcggat agctcctgcg actcaatcag ggcgaaaagc tcctgaccta cggcgcgcgc
      781 ttcgcgcaaa acgcgcaaga gctgcgaatc cagagtcgcg atcgcctgca cgagcgggga
      841 cttctcgctg ccgagcagcg gcggaaggga agagaaggaa ggtcgcgcga gctcatgcac
      901 gccgagcact tcgctcaccg cgagcgctac ggcgcgcgtt ccgaccgaca ccgtcaggta
      961 acgcccgatg cgagccgtcg gcgcgccggt gaccaaaagc gcgaggtcga cgaccgggac
     1021 gagcgtgcct cgaatcatgg acatgcccag aacgaaaggc ggcgcacgcg gcgacggctc
     1081 cagcgccaac gggcgcatca cctcgttcag tcccacgagc ggcaacgcgc aagcgctgtc
     1141 tccgcagcga atcacgagca ccctcaaacc cggtcggttc gacatgacgt tcctcgaccg
     1201 ctccaagtca gttcaacgcg tggcgcagcg tgtcgtcgaa gacctcgcgc acgcgccgca
     1261 agagcccctc ggcgctgaag ggcttttgaa gaaagtattc ctgtgctcca tcgccgcccc
     1321 gcggctgggt agccgccgca tccatctgcc ccgacatgag cagcactctc agcgccggtt
     1381 gcagcgcgcg caaccgcgct gggagttggt ggccgctcgg gggcgacgct acgtcggtga
     1441 gcagcaggtc gatgggctct ggtgacgact cgcacaggat gagcgcctcc cgggcgccgc
     1501 gtgcctcgaa cacgcgatag ccacggcgcc gcaagacctg cgccgccgcg cgtcgaacct
     1561 cctcttgctg ctctacgagg agtatcgttt cgctgccttc gagcttcatt ggcggcccga
     1621 gcgtgttcac cgagctcgcg gcctcctccg tcgcgggcag gatgatccca aagcgcgtcc
     1681 ccttgccgag cgcgctgtcc accctcaggt ggccatggct ttgcttcacg atgctctcga
     1741 ccgtcgcgag gccgaggccg gtgccctttc cgaactcctt ggtcgtgaaa aaaggctcga
     1801 aaattcgcgc gatcgtcgcc gaatccatcc cgactccagt gtcgctgacc gtgagcatca
     1861 catatcggcc cggctccgca tggggccact cgtcgcgcgg agccgcggag agctcgcagt
     1921 tgctcgtcgc gatgagcagc tcaccgcctt cgggcatcgc gtcgcgcgcg ttcacggcga
     1981 ggttcatcac cacttgctcg atctgccgcg ggtcggctcg gacacaccag ctaccttgcg
     2041 aatggacccg aagctcgatg ccatccccca acaggcgctg gagcagtttg gccgagtcgc
     2101 gaacgattgc gttcacgtcg accacgcgaa ccgagggtac ttgagcgcga ccgaacgcga
     2161 gaagttgacg agtgagctcc gccgcgcgct cccccgcccc tcgtatcgct tccacgtcgg
     2221 cgcgaaccga agcgtccgcc ggcaattcgt tcagggcgag gctgctgtag ctgaggatca
     2281 ccgacaagag gttgttgaaa tcgtgcgcga cgctggccgc gagtcggccg attgcctcca
     2341 atttctgcga ctgcgagagc ttcttctcca gctgtcgttg agcggtgacg tcgtgcaaca
     2401 acgctatgct gccgcgtaga tttccgtagt cgtctcgaag gggcgtggcc gtgaccgcga
     2461 cgttcgctcc cgaaacccgc gcgttcttga cgaacacctc ggcttcggtc cgttcttcac
     2521 ccgccatcgc ccgcagcagc gggttctctt cgctggcgag cggagcctga tcgcgtccgt
     2581 agatgccgta cgcttccgct agcccgactg gctccacctc aggcggaccg atgccgagga
     2641 tcttctcggc gcgccggttg aacagcacga cgtgcccgct tcggtcgagc acgacgactc
     2701 cctccccgat tccagccaaa atcgagttca gaagggcggt ctgttcgcgg tagcgtgcct
     2761 caccctgtcg cagcgcttcg tcgccacgga cccgctccgt gacgtcgcgc gcaatgccga
     2821 cgatgccgac cacctcgccc gactggttca ggagccgcgc gcgaatttcg gagaaccagc
     2881 gcgacttctc ggagctcccg gcacgacgca gcgcgtcctc cgccgcctcg tcaccgccga
     2941 gcacgagcgc ctcttctcgc tcggccgctt cgctttcggc accggggacg atctctttca
     3001 gactcttgcc gaccaagttt tccggcacgg cgacgcccag cgagcgcgca aatgctcggt
     3061 tggcgcggac gaacctgagc gccgcgtcct tgaagtagat tccatccgag acgctatcca
     3121 tcaagccctg gagcaggcgg cgctcctcca tgagctcccg ggcgaggcga acgtgttgca
     3181 ccgcgcggcg caccttcagg cgcaggtcgt cgtcgttcca cggcttcgcg acgtaggcaa
     3241 atatcccgcc gtcgttcacg gctcggatca cggcggacgg ctcggcgaaa ccggtcacca
     3301 gaatgtgagc catgttggtg gtgatggcta ggcgcgcgag cagctcgtca ccggtcatgt
     3361 gcggcatccg ctgatcggtg agcacgaccg cgatttcggg gcggtgcgcg acgatctcga
     3421 gcgcacgcgc ggccgagccg gccttcacga tctcgtactc ctctgacaac acgtcctcga
     3481 gcgccacgag aacctggggt tcatcgtcca cgagcagcac ggtttccctt gcctgcatca
     3541 gctccttcct ctcagcgcta agaaatcggg atccgaatga tggctcgggt cccaccgcct
     3601 tctcggtccg taaggacgag cgcgccccgg tgcttctcca cgatggagta cgtgatggag
     3661 aggccgagcc cagtaccttg tccaaccggc ttcgtcgtga agaagggctc caagactcgc
     3721 tcccgcagcc cgggttcgat gccgggcccg ctgtcttcca cgatgatcga gagctccgag
     3781 tcggttgcct cggtctcgat tcgaatcaaa cccggttcgt cacccagcgc atcgattgcg
     3841 ttggtcacga ggttcatcac cgcctggttg agtgggcctg gatagcaatc gacgaggtcg
     3901 cgcgaagcga acttggtttc gacgcgaatg ttctcgttca aacgatggtt caagatcgtg
     3961 agcaccgact cgacgcactc gcggatactc gcgcgtttcc gttcgccctc gtccaggcgc
     4021 gaaaacgtgc ggagcttcac gacgaggtcg cgaattcgct ccaggccggc gtcgatttcg
     4081 gagagccgat tcttcgcgcg gagccagttc tcgtcgtcgt gggcgacctg tgcttcgact
     4141 ttgtccaggc tcttccgcgc cgtcttgagg tgactcagcg cgaacgagag ggggttattg
     4201 atctcgtgag cgacgccggc cacgagctgg ccgagcgagg ccattttcgc cgattgtacg
     4261 agctgggcct gggtctcctg cagcgcttga taagcagacc cgaggtccct gttcttgcgc
     4321 tcgagctcgt ccacgagcgc cgcacgcgtc gcggccagct ctcgggccgc gcgcgcctcg
     4381 gctgcttcga gctcgccgcg cagcagctgc tcgcgtacgc gacgagtctc gtcctccacc
     4441 tgtcgacgac gcagctgagc tcgaacgcgt gccttcagga tctcgaacga tgcagacttc
     4501 tctacgtagt cgtccgcccc cgccgagagg ctttccagcg tcgtcgaccg gtcgtcgaag
     4561 gcgctcgaca tcacgatggg gatatcgcgg atcaaaggcg aattcttgag ccgccgacac
     4621 gtctctcgcc cgtcgatacc ggagagctcg gcgttcagca aaatgcagtc gaccgagtgc
     4681 accgccagta gatcaatcgc ttcttccccc gagcgcgccg agatcacgtc gtagccctct
     4741 tcgcgcagtg cattggcggc tgcttcgcga tagccgtcct cggtatcgac cgccaaaatt
     4801 tgtttcgggc cgagcaagct gcgcgtcgtc gactccaagc tcgagtcgct cgcggagcgg
     4861 agcagcgcct tcagcttggc gagcatgatg tcggggtcgt cgcccttgtg gacgaaagcg
     4921 tccgcgccgg agtcgagcgc gcgtagctct tcggagaccg cgccgtctcc cgtcaacagc
     4981 aggcatggaa gccgtcgcag cgccgcgtcg agccgaaccc gtcggatcac ggccgcccca
     5041 tccattcctg gcagcacgct gtcgactatc agcgcgaccg ggcgcacctc atccaagaag
     5101 cgaaggccct cttctccgct cgccgccgtc agaaccttgt agcccgcgcg agccagcact
     5161 tgcgagagct cggcgcgcgc ggtgagactg tcttcgatga gcacgaccgt cttgccgtcg
     5221 ctagcgcgag aatttctgcg caagagttct ctcgcgcgtg cgatgacgta tccgcgatcg
     5281 tacggcttac cgacgtagtc gttggctcct gtcttcagcc cccgaatgcg atcgctgatt
     5341 tcggattcgg tggagagcat caaaaccggc gtctccgagc ccgcggacga gcgcagctcc
     5401 cgcagcaggt cgactccgtc tccgtccggg agcacgacat ccaagatcac gacgccgacg
     5461 ttttgctcgg cgaggagctt tcgagcctcg gccaaggtcg cgcacgagag ggggcggagg
     5521 cccacctttg caaacgcctc cgtgaggtcc atacgcacgg tcaagctatc gtctacgatc
     5581 aggatattcg cggtcacaac gcttccccgc gtgcgccgtt gcagccgccg gcccgtacga
     5641 aaatggccct ctttatctcg caaaacggcc ctttgcgaga gccagaaacg tactcctcct
     5701 cgaaggcata ggtcaagaat cactctcact cgtgatcatc gtacggatac acggagtgta
     5761 cgaatcgacg tcatcggttg ctggcgactc ccgactttgc ggtggcgcgc cgttcaacaa
     5821 ggacgcctcg gttgcgacaa caacttcgtc gcacgggcgg tgacaactta ttggctctgg
     5881 cccgcgcgag cggttcgcca gcgtggcctg gaacttgcca aagcgtactg agcgctcgcg
     5941 taccgagcgc tctacggagg tcgcagtctc atggttcttt cgcttggtaa atcgctttca
     6001 gtcgtcgcac cccttggtta ttgtctcctc gccgcgtgcg gttcggacca tcagtcgcag
     6061 cacgccaaca cgccctcggg cgccgtccag gaagtatcgg cgagcgccga gacgcgctct
     6121 gccaccgaat caatctccga agcgcgttgt gtgcgcgagc aacgctgcga caacgtcggg
     6181 gacaacaaga agtactcctc gatgtcggat tgcatgacgc gcatccgcgc cgactg
//
LOCUS       DASTKS010001260         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1097068, whole genome shotgun sequence.
ACCESSION   DASTKS010001260 DASTKS010000000
VERSION     DASTKS010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097068"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..1176
                     /locus_tag="VFQ35_26920"
     CDS             514..1176
                     /locus_tag="VFQ35_26920"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HET9934376.1"
                     /translation="MAPPAGSLDEQRRRVIAGRKHGRRRKLERGQTERRSIIWRGFGV
                     RRRAVHGRRVIASRYLDEQRGSLERRHEFDRRRERKPGRKRWHDGQLPAAQDSGIQGT
                     SQNLFEAVQVYTIQGSNKYLMIVEAQGNARYFRAWTATDLEGPWTLVTDNFAIKSNVT
                     FSTNWTNDISHGDLVRSSPDETFPVDPCNLQLVFQGRDPKANVSDYGLLPYKLGLLTL
                     AK"
     gene            1550..>2561
                     /locus_tag="VFQ35_26925"
     CDS             1550..>2561
                     /locus_tag="VFQ35_26925"
                     /inference="COORDINATES: protein motif:HMM:NF017369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HET9934377.1"
                     /translation="MTHRVERARRAMMKTAPFLAVLALASACSDNGAEGTDSSADASR
                     VEGTLETVVVGNAERTAAHLEFFVEDASGRWTRLLFDEHPEFIHDVDGHAHLIVPGHS
                     HDELTGSVTLRVTGARTFDTLQVSSLEIIDPAPHDTASTEQALIASSPHKVAVILANF
                     SNDTSQPITAAAARDMVFGSASSANAYYQSVSFGIRSLVGKLATSGDVFGWYTLAAKN
                     DACDYASWGTAARAAAQAAGVDLSGYDHVIHYFPRAASCGWAGVGQVPGKYTWINGSN
                     SQTVAHELGHNFGSHHASTLICTNSSGARVSISPTCTLNEYGNPFDVMGSGYHHLTAF
                     NKG"
BASE COUNT          488 a          848 c          823 g          402 t
ORIGIN      
        1 cgacccggcg gacgcggcgg cgccgccgaa cgacgaagta ccgccagacc cgagggtgac
       61 gtgaccgccg gacgccgcgc tcacctcgcc atcccagtcc agctcagagc cgctgcgcgc
      121 gccgcaggca accaaaacca cgccgagcac cgcgactgcc gcgcatgaat caccagcgcg
      181 ccctcggcga ggccctccta accgcccaac ccgctgagca acccggccga ccatcacgcc
      241 gcgagcgtag cagaacgagt gtgcgctcaa ggctcaaccg agagagcgga atttttgcac
      301 tcgaccggat gaacctttcg ttgatggttt ggcgacttcg agcgcactgc ttgggcgggt
      361 tagcgccgat cttatccatg aaatccagtg ccaaagccac tgccttgaac ttgatgcgag
      421 cgctcctcgc gacctccctc tgcgttttct cggggggctg ttcgagcgat tcgggcaccg
      481 gtggcacctc gacgcgcggt ggcgcgagtt cgggtggcgc cgccagcggg gagcctcgac
      541 gagcagcggc ggcgcgtcat cgcagggcgg aaacacgggc gcaggcggaa actcgagcgg
      601 ggacaaaccg agcggaggag catcatctgg cggggcttcg gcgtccggcg gcgcgcagtc
      661 catgggcggc gcgtcatcgc aagccggtac ctcgacgaac agcgggggtc gctcgagcgg
      721 cggcacgagt tcgaccggcg gcgggagcgg aagcctgggc ggaagcggtg gcacgacggc
      781 cagctcccgg ccgcgcaaga cagtgggatt cagggcacgt cccaaaacct gttcgaggca
      841 gttcaagtgt atacgatcca gggctcgaac aagtacctaa tgatcgtcga ggcgcagggg
      901 aacgctcgct acttccgagc gtggacggca accgacctgg agggcccgtg gacgctcgtg
      961 accgacaact tcgcgatcaa gagcaacgtg acgttcagca cgaactggac gaacgacatc
     1021 agccacggcg atctggtgcg cagcagcccc gacgagacgt ttccggtcga cccctgcaac
     1081 ttgcagctgg tcttccaggg acgtgatccg aaggcgaacg tgagcgacta cggccttctg
     1141 ccctataagc tcggactcct cacgctcgcc aagtgagagc ggttcaacgc tcgagtgagg
     1201 gccccggagg tgctgcgaga ggcgcctccg gggccgtcgc gttgcaccgg tagctctgcg
     1261 cgcgagcgat ccgacgcttc acgccgggac caacgcgccg ccgaagtgag ctcgctcggc
     1321 acacccacct agcgatatgt atcgtgctcg tggtttgggc tggcgcgctc tgcgaagcat
     1381 gtaaagctcg actcctcacg gttcggtatc gctgccgtgc gcgcgcaggc gttgtctgaa
     1441 aagattcaac gactcgctcg tgctccgtca gcttcaaaaa acacggaatc agagcgtgta
     1501 gcgcctcggt tcgcggggcg tcgcgcgctc gtctacgagg gaccatcgaa tgacacatcg
     1561 tgtggagcgc gcgcgtcgcg ctatgatgaa gacagcgcct tttctggcgg ttttggcatt
     1621 ggcaagcgcc tgctcggaca acggcgccga gggcaccgac agcagcgccg acgcgagccg
     1681 cgtcgaaggt acgctggaga cggtcgtagt tggaaacgcg gagcgaaccg ccgcgcacct
     1741 cgaattcttc gtggaggatg cgagcggacg ctggacgcgc ctgctcttcg atgagcaccc
     1801 cgagttcatc cacgacgtgg acggccacgc gcacctgatc gttcccggac attcccacga
     1861 cgagctcacg gggagcgtca ccctccgcgt cactggcgcg cgaaccttcg acacgctgca
     1921 agtgagctcg ctcgagataa tcgaccccgc gccgcacgac accgcgtcga cggaacaggc
     1981 gctgatcgcg agttcgcccc acaaagtcgc ggtgatcctc gcgaatttct cgaacgacac
     2041 ctcgcagcca atcacggccg cggccgcgcg cgacatggta tttggctcgg cgtcgtccgc
     2101 gaatgcctat taccaaagcg tctcctttgg aatccgctct ctcgtgggca agctcgcgac
     2161 gagcggcgac gtgttcggct ggtacacgct cgcggccaag aacgatgcgt gcgactacgc
     2221 aagctggggt acagccgcgc gcgcggcggc ccaagccgca ggggtcgacc tctctggata
     2281 cgaccacgtc atccactact ttccccgcgc cgcgtcgtgt ggctgggccg gtgttggtca
     2341 agtccccggc aaatacacgt ggatcaacgg gagcaactcg cagacggtcg cgcacgaact
     2401 cggacacaac ttcggctcgc accatgcgag caccctcatc tgcacgaact cgagcggcgc
     2461 tcgcgtgagc atctcgccga cttgcacact caacgagtac ggtaacccct tcgacgtaat
     2521 gggcagcggt tatcaccact tgaccgcgtt caacaagggt c
//
LOCUS       DASTKS010001261         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_204401, whole genome shotgun sequence.
ACCESSION   DASTKS010001261 DASTKS010000000
VERSION     DASTKS010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204401"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VFQ35_26930"
     CDS             <1..1096
                     /locus_tag="VFQ35_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934378.1"
                     /translation="PLNGLRFGANRSLGVKVDFIPSLASFEMELGSASMDLSLLKDQK
                     SGYLSGVIYPGKSFFNDVVPILPQTALKVAGAIASPISDSRFAMGGDVALKLSKLNTM
                     LNLSLSDVPLASAELGIDKNGLSMRGNAQASLISALGMNGQATFDASFPGDLETSAWY
                     VDMNGNLSVHGVGLSGATRAHLDRSGLQVDGRLATALGGIAVTGRIDQNGATLRGRAN
                     VSIPMVAGATVVSNAVDGVACGYEKITSAAVCGADTLEGWSKCGVKFVASCLVSSCET
                     PKCSIPHTCDDFSRPKACPHNVINPNFNYGTFQGTVDVTIGPGGVQATLSGVCVAGQC
                     SSSPTTSVDLSSSQACVQLPGISQKFCAKF"
     gene            complement(1171..>2051)
                     /locus_tag="VFQ35_26935"
     CDS             complement(1171..>2051)
                     /locus_tag="VFQ35_26935"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9934379.1"
                     /translation="ESGSGKELVARRVHELSPRAAGPFIKVHCAALPDNLLESELFGY
                     EKGAFTGAVTRKPGRVELSQGGTLFLDEIGDITPAVQVKLLRLLQEREFEPLGGTRTI
                     KADVRFVTATHRDLEGLVAQGKFREDLLYRLNVVSLWVPPLRARPEDIEELALHFCAT
                     TASANGRSSLALDVAALDLLKQKPWPGNVRQLQNFVERLVVLAEGPRIRAEDVERELG
                     RQAGTMGFAVTSGFTPPVTMESSLVDLNAALRKAERRALEKALKSANGNRTVAARLLG
                     ISVRSLYYKLEEHELS"
BASE COUNT          360 a          660 c          659 g          372 t
ORIGIN      
        1 cccactgaat gggttgcgct tcggagcgaa ccgttcgctc ggcgtcaagg tcgacttcat
       61 cccgtcgctc gcatcgttcg agatggagct cggctcggcg tcgatggacc tctcgctgtt
      121 gaaggatcaa aagagcggtt acctctccgg tgtcatctat ccgggcaagt ccttcttcaa
      181 cgacgtggtg ccgattctgc cgcaaaccgc gctgaaagtt gcgggggcca tcgcgagccc
      241 gatctcggac tcacgcttcg cgatgggggg cgacgtcgcc ctcaagctct cgaagctgaa
      301 tacgatgctg aacctctcgc tttcggatgt cccgctcgcg agcgcggagc tcggcatcga
      361 caagaacggg ctcagcatgc gcggcaacgc gcaagcctcc ctaatcagcg cgctcggcat
      421 gaacggacaa gctaccttcg acgcctcgtt cccgggcgat ctcgagacca gcgcctggta
      481 cgtcgacatg aacggcaacc tctcggtgca cggcgtcggt ttgagcgggg cgacgcgcgc
      541 gcacctcgat cgcagcggcc tgcaggtaga cggtcgactc gcgaccgcgc tcggcgggat
      601 tgcggtaacg gggcgcatcg atcaaaacgg cgccacgttg cgcggccgag ccaacgtcag
      661 catcccgatg gtggccggcg cgaccgtggt ctcgaacgcc gtcgacggtg tggcgtgcgg
      721 ctacgaaaag atcacgagcg cggccgtctg cggcgccgat accttggagg gctggagcaa
      781 gtgcggcgtc aaattcgtgg cgagttgctt ggtgtctagc tgcgaaactc ccaagtgcag
      841 cattccgcac acctgcgatg atttttcgcg gcccaaagcc tgtccccaca atgtcatcaa
      901 cccgaacttc aactacggca cgttccaggg cacggtggac gtgacgatcg ggccgggcgg
      961 cgtgcaagcg accctcagcg gcgtgtgcgt cgcggggcag tgttcgtcct cacccacgac
     1021 ttcggtcgac ctgagctctt cgcaagcgtg cgtgcagttg ccagggatct cgcagaagtt
     1081 ctgcgcgaaa ttctgagcta accggtcgaa acgacgccgg ctcctcacgg gccggcgtta
     1141 cccattttgt agagggtctc gacgatgcgc tcagctaagc tcgtgttctt ccaacttgta
     1201 atacaggctg cgcacgctga taccgagcaa gcgggccgcc acggtgcggt tcccattcgc
     1261 gctcttcagc gccttttcga gggcgcgccg ctcggcctta cgcaaggcgg cgttgagatc
     1321 gacgaggcta ctttccatcg tgaccggcgg agtgaagccg cttgtcaccg cgaaacccat
     1381 ggtgcccgcc tggcgaccga gctcccgctc gacgtcctcg gcgcggatac gcgggccttc
     1441 ggccaagacg accaagcgct cgacgaagtt ttgcagctgg cgcacgttgc caggccaagg
     1501 cttttgcttg agaagatcga gcgcggccac gtcgagcgcg agcgaggagc gcccattcgc
     1561 ggaggccgtc gttgcgcaaa aatgaagcgc gagctcctcg atgtcttccg ggcgcgcgcg
     1621 cagcggcggc acccaaagcg agactacgtt gagccggtac aagagatctt cgcggaactt
     1681 gccctgcgcg accaagcctt cgagatcgcg gtgggtggcc gtgacgaagc gcacgtccgc
     1741 tttgatggtg cgcgtgcctc cgagcggctc gaactcgcgc tcttgcagca ggcgcaagag
     1801 tttgacctgc acggcgggcg taatgtcgcc gatttcgtcg aggaacagcg ttcctccctg
     1861 cgagagctcc acgcggcctg gcttgcgagt cacggcgccg gtaaacgcgc ccttctcgta
     1921 gccgaagagc tcgctctcga gcaggttatc gggcaacgcg gcgcaatgaa ccttgatgaa
     1981 gggaccggcc gcgcgtggcg acagctcgtg aacgcgccgc gcgacgagct cctttccgct
     2041 tccgctctcg c
//
LOCUS       DASTKS010001262         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_425761, whole genome shotgun sequence.
ACCESSION   DASTKS010001262 DASTKS010000000
VERSION     DASTKS010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425761"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFQ35_26940"
     CDS             <1..396
                     /locus_tag="VFQ35_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934380.1"
                     /translation="ETVIPEALEPNPELQSEPEPVGSARPAPSAASSAPHAATGADLA
                     REIAVLDSARRALAAGNPAEALNRLDGEQPLSVRTLAPEATVLRVRALLRLGRRSAAR
                     AVVERFAHDAPGSPQLAVLRELLHADSNL"
     gene            445..1146
                     /locus_tag="VFQ35_26945"
     CDS             445..1146
                     /locus_tag="VFQ35_26945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LDL receptor domain-containing protein"
                     /protein_id="HET9934381.1"
                     /translation="MIRLTSALTVPLFAIACTQLPVDIGDKLPDDNQGSGSGARTGGG
                     GSTSTAGGSNSGGLTNTGGYSTGGTGTWPGTGGVSSGGAGGSATGGFTSGGYTSGGGT
                     GAWTGTGGTTTGGMGGGGATFDCGDNQLIPIERLCDRSFDCLNGTDEDPMYCDGSGTF
                     TCQSGQLIAAALKCNGMADCGDGSDELRSACGEVFAFRCADGTAVPAEKICDGLLDCA
                     DGRDEDMVMCPRGPR"
     gene            1365..2216
                     /locus_tag="VFQ35_26950"
     CDS             1365..2216
                     /locus_tag="VFQ35_26950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934382.1"
                     /translation="MAKFWMIGLSCLALASGCTPGREAKTAGQIGCTPDEITISNHES
                     HFGLVQSGDTWVAECQGRTFVCSQLNEAGNDKGFFDSLFASEQVSCHEAPESQEAMRR
                     RITKEVAATEQARRPAPSAPTGAAGFAFGASPEEAAKGCESAGYAWRSGGEGAPSGCS
                     GPAALLGISANVNIRFCEGHTCAITVEHVPRSHWMQGSVSLKANLESKYGPAQESSGI
                     IPEQCRSERGLTRCLESRALTLRYVWRWASGESLEMSVGKPSEAAISAIRLEYRRVAG
                     AANLSAL"
     gene            complement(2336..3529)
                     /locus_tag="VFQ35_26955"
     CDS             complement(2336..3529)
                     /locus_tag="VFQ35_26955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934383.1"
                     /translation="MPSHSGVEHDLFEAPPPPPGEHPPEASDAAVDLSDIALSERPPR
                     NDSAPPLFDLAHNGPAKPAGVQEDIDFLLGLQGSRGTTATLQNPTLADLAKKPPSEPP
                     PFPIDVEFPVAQAEPAPVAVKPRTSTAAEAFAPPPPAQHSAAPHVTSVSASPQLKSRK
                     RPAALFAVAALGLSGAVLASTMLTHRADVAPPAALPTVSPEPPPTSARATRSGSFDSD
                     EPASPPLEIPASGETSTPRAPSARAQAANPRVPSRPATTGTKPPLAEKTTPPSTTIAR
                     NEPATPTPARVEPARPTPPITPRPSEPRAPVGAGFDRQAAVNALNTQAGQASSCRKGE
                     DPSGTATVVITFAPSGRVTSANVSGPPFAGTPTGGCIAAAFRRARVPEFDGDNITVSK
                     TVVIH"
     gene            3699..4118
                     /locus_tag="VFQ35_26960"
     CDS             3699..4118
                     /locus_tag="VFQ35_26960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934384.1"
                     /translation="MVTGLRGGVGFCAGGGCPVGFFGGAGFCEAVGFSGGFNLWVADA
                     TTAMVGAVGAGALGAAVGVGAGVVAFVAGAVVGGAAARDGAGCSVLGAFFSGGRGGVG
                     ATAGDEALGEDGSGSGSGGCDAVKRMSSPQRLQRNAS"
BASE COUNT          666 a         1292 c         1632 g          739 t
ORIGIN      
        1 gagaccgtaa ttcccgaggc gctcgaaccc aacccggaac tgcaatccga acccgagccg
       61 gtcggcagcg ctcgccctgc gccgagcgcg gcctcgtccg caccgcacgc ggcaaccggc
      121 gccgacctcg cgcgggagat cgccgtgctc gactccgctc gccgagccct cgccgcgggc
      181 aacccggccg aagccttgaa tcggctcgac ggcgagcaac ctctcagcgt gcgcaccctc
      241 gccccggaag cgaccgttct ccgcgtgcgt gccctcctcc gccttggtcg gcggagcgcc
      301 gctcgcgccg tcgtcgagcg attcgcccac gacgctcccg gctcacccca acttgccgta
      361 ttgcgggaac tcctccatgc ggattcgaat ttgtgatcca aaaccagtgg tgccgtcttt
      421 gaacgggcgg aggttgtgat gaacatgatt cgactgacgt cggcgctgac ggttcccctc
      481 tttgcgattg cctgtaccca gctcccggtc gacatcggcg acaagctccc ggacgacaac
      541 caaggttcgg gctctggcgc gaggacgggt ggcggtggaa gcacttcgac cgcgggcggc
      601 agtaatagcg gtggcctgac gaacacgggt ggctacagca ccggtggcac cggcacgtgg
      661 cccggcaccg gcggcgtcag cagtggtggc gcgggtggct ctgcgacggg cggcttcacg
      721 agcgggggtt acacgagcgg cgggggcacc ggcgcttgga cgggcacggg tggcaccacc
      781 actggtggga tgggaggcgg cggagccacc ttcgactgcg gagacaacca actcatcccg
      841 atcgagcgac tctgcgacag gagcttcgat tgcctcaacg gcaccgacga agatccgatg
      901 tattgcgacg gcagcggcac cttcacttgc cagagtggac agctcatcgc agctgctctc
      961 aagtgcaacg gcatggccga ctgtggcgac ggcagcgatg agctccgctc tgcgtgcgga
     1021 gaggtctttg cgttccgctg cgcggacggt actgcggtgc ccgcggagaa gatctgcgac
     1081 ggcttgctcg actgcgccga cggccgagac gaagacatgg tcatgtgtcc gcgcggaccg
     1141 cggtgagctg aactcggagc tgacgacatg gcggctcctt gataagggga gccgcgtgtc
     1201 ccgctctgcg agcgtggctc cccttcgatg cgttgctgcc gccggggtga ggcagcgcat
     1261 tcacagggcg cgtaccgtcg attacggatc gcgcttcgcg agcctactct cgactccttt
     1321 ggcgcctggc atgcgaggtg ctctgcgtcg acagaggcag gcacatggcg aagttctgga
     1381 tgattgggct gtcttgtctc gctctcgcga gcgggtgcac gccgggtaga gaggcgaaaa
     1441 cggctgggca gatcggatgc acgccggacg agatcaccat ttcgaaccac gagagtcact
     1501 ttgggttggt tcagtcggga gatacgtggg tcgcggaatg ccagggccgc acgttcgtgt
     1561 gttcgcagct gaatgaagcc ggcaacgaca agggcttttt cgattctctg ttcgcgtcgg
     1621 agcaggtgag ctgtcacgaa gcgccggaat cgcaggaggc gatgcgcaga cggatcacga
     1681 aggaggttgc ggccaccgag caagcgcgtc gcccagcacc cagcgcgccg accggcgccg
     1741 cggggtttgc ctttggggcc tcgcccgagg aagccgcgaa gggctgcgag tcagcgggct
     1801 acgcctggcg gagcggagga gagggtgcgc cgtcagggtg ttcgggcccg gccgcgctgc
     1861 tcggtatcag cgccaacgtg aatattcgat tttgcgaggg gcacacttgc gcgatcacgg
     1921 tcgagcacgt gccgcgctcc cactggatgc agggctccgt ttcgctcaaa gcgaacctcg
     1981 agagcaaata cggtcccgcc caggagtcga gcggtatcat tcccgagcaa tgccgctccg
     2041 agcgagggct cactcgctgc ttggagtcgc gggcactgac gctgcgctac gtgtggcgct
     2101 gggcgagcgg tgagtctctg gagatgagcg tcggcaaacc gagcgaagcc gcgatctctg
     2161 ccattcgcct cgagtaccgg cgagttgcgg gcgcagcgaa cctatccgcg ctttgagtcc
     2221 gtggtgagcc ggcctcacgg cgaggcgctc gcggtcgtta ctcggaaggc aaagacgtgc
     2281 tctgggtcgc cggtgagcgc gccgcgaagg tcatgactct gcggcagagc cgcgctcagt
     2341 gaatgaccac tgtcttgctg acagtgatgt tgtcgccgtc gaattcgggg acgcgcgcgc
     2401 ggcggaacgc ggccgcaatg cagccaccgg ttggggtgcc tgcgaaaggc ggtccgctca
     2461 cgtttgcgct cgtgacgcgg cccgatggag cgaaagtgat gacgacagtc gcggtgcccg
     2521 aagggtcttc gcctttgcgg cagctcgagg cttggcccgc ctgggtgttg agggcgttga
     2581 cggcggcttg gcgatcgaac ccagctccga cgggcgcgcg tggctccgag ggccgcggtg
     2641 tgattggtgg tgttgggcgc gctggttcga ctcgagcagg cgtgggcgta gccggttcgt
     2701 tgcgcgcgat ggtggtcgag ggaggggtcg tcttttcggc caggggaggc ttggtgccag
     2761 tggtcgcggg gcgcgaaggg actcgcgggt tggcggcttg ggcgcgtgct gacggagcgc
     2821 ggggcgtcga cgtttcgccc gaggcgggga tctccagcgg tgggctcgca ggttcgtcgg
     2881 agtcgaacga accgctgcgc gtcgcgcgag cactcgtcgg aggcggctcg gggctgacgg
     2941 tgggaagtgc ggcaggaggt gcgacgtcgg ctcgatgcgt gagcatcgtc gaggcgagca
     3001 ccgcgccgct caagccgagc gcggcgaccg caaagagtgc cgccggacgc ttgcgcgatt
     3061 tcagctgcgg actggcagag acactcgtga cgtgcggtgc tgccgaatgc tgcgcgggcg
     3121 gcgggggagc aaacgcttcc gcggcggtcg acgttcgggg tttgacggcg acgggcgcgg
     3181 gctcggcctg ggcaaccgga aactcgacat cgattgggaa ggggggaggc tccgaaggcg
     3241 gcttcttggc gagatccgcg agcgtgggat tttgcagcgt cgccgtggtg ccgcgactgc
     3301 cctggaggcc caagagaaag tcgatgtctt cttgaacgcc cgctggcttc gcggggccgt
     3361 tatgagcgag gtcgaaaagc ggcggcgcgc tgtcgttgcg aggcggccgt tccgagagcg
     3421 cgatgtcgct caggtcgacg gccgcgtcgc tcgcctcggg cggatgctct cccggtggcg
     3481 gtggtggcgc ttcgaacaga tcatgctcga ccccgctgtg actcggcatc gttccgcgat
     3541 ggagcgcgac gggcttacgc tgcgggcgca gcggcggacg tggtggggct ggagtcattg
     3601 ccggcgctgg tgcgggcgcg ctggttaccg ccggcgtcgg cgcgcgcgat ggcgccgcag
     3661 gcgagccgcg cagccctgga ggagcaccga ttccttgaat ggtcaccggc cttcgcggcg
     3721 gggtcggctt ttgtgcgggc gggggttgcc ccgttggttt tttcggcgga gccggctttt
     3781 gcgaagcggt tggcttctcc ggagggttca acttgtgggt ggcagacgct acgaccgcga
     3841 tggtgggcgc ggttggcgca ggtgcgcttg gagcagcggt gggcgtcggc gcaggagtgg
     3901 tcgcgttcgt agcaggagcg gtggtgggcg gcgctgctgc gagagatggt gcgggttgct
     3961 ctgtgctcgg tgccttcttc agcggagggc gcggtggggt gggtgcgacc gctggagacg
     4021 aagcgttggg ggaggatggg tctggaagtg gctcgggcgg atgcgacgcg gtgaagcgga
     4081 tgagttcacc gcagcgtttg cagcgcaacg ccagctgact gccgagtcgc ttttgtacca
     4141 agtcgtttgc gacgctgaag cgagcaccac aagacttgca cgtgaccgcg taggccaaag
     4201 gcggaaccac tccgaggttc gcgttgcgcc tcgccggagg gacctcgaac ctcggcgcgc
     4261 tctaccagaa gcaagacagt atcccccaga acacacgagc ttcgcaagcg aaggccgtca
     4321 gcgggcaac
//
LOCUS       DASTKS010001263        10554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_649672, whole genome shotgun sequence.
ACCESSION   DASTKS010001263 DASTKS010000000
VERSION     DASTKS010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649672"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1207)
                     /locus_tag="VFQ35_26965"
     CDS             complement(302..1207)
                     /locus_tag="VFQ35_26965"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET9934385.1"
                     /translation="MANVTFTSPRMSRDVTVYAVAGDRGTILALAKEHKIPIPFDCQD
                     GECGSCLVKVTQVSSARPYGIALTEKEKELLKQLGKITREEVMNAEVNDMPPRYRLAC
                     QCFVRNEDVLVEFEGDTTLPEQGPHLSIAAEVYKGGITISSLDDFLCFAVKVEEDAAL
                     HFEKLGNNMEACGNADVAALFRQLATYSRMHHAEAKARTAAHSVELKLPTNSAWPDHE
                     TPERTALWAGDPRLSRLDALKAALQGERRGYEFYYAVANTTADVEIRKVAREFVREEA
                     QHVDTLKLWIAKEEARVQEAARAAL"
     gene            1447..1920
                     /locus_tag="VFQ35_26970"
     CDS             1447..1920
                     /locus_tag="VFQ35_26970"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET9934386.1"
                     /translation="MENLQVFLAHAVQLERDAARRFEDLMHSMHSAGNYEVEALFRRL
                     GEFSRSHLKAAMARSGFQVLPVLAPGEFQWPEGTSPEAAGWRGVDGALDVTGALELAL
                     EGEESGMRFYEAIARTSSNPEVTHLAREFADEEWGHVTELRRWIARATANGAELR"
     gene            complement(1977..3413)
                     /locus_tag="VFQ35_26975"
     CDS             complement(1977..3413)
                     /locus_tag="VFQ35_26975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934387.1"
                     /translation="MHTYARSLTALFALLVGAAPIGCTVESSSDGSGGTDAGAAGHAG
                     ASGSGSGGNSAGAGGGGGSGARGGSGGSANCEAGLTGALDLVVEGLPPSVPASIAITG
                     PAAKTATASKTFATLPSGVYSIESRAVTDATPVVRSLYEAAPRSVDVCVSPANAQSTV
                     SYSAVPTSGKLWVVSSEDANLQGFAAASLGASNASPPALAMDAKAGGDIAFDRDGNVW
                     TFGPTLAEAPLLRYPHTAFAASGPALPDRELVLADQPCTTLHAMAFAPKGSLWVSSCG
                     VVLRYDAATLAAASGEVSADAIISNLDDNGDVAFDRAGNLWVTDAGLVKRYDLSRLAA
                     STGAAADAVFTVRNASDTADLAASNLLFDADGDLWIVDFGGNLLSEVKASTLAGVGAH
                     SVPSSVTIALSVTALLSRPAFDDAGALWISYQRGSVARVAPEALLVSTDAGAPTTPEV
                     ILSSSTIGYASNVAFFPAPAGLPLYHSF"
     gene            complement(3597..4715)
                     /locus_tag="VFQ35_26980"
     CDS             complement(3597..4715)
                     /locus_tag="VFQ35_26980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET9934388.1"
                     /translation="MWHKRTQARPRRSHERRRALEAVGAFRAAPNWFAFGTLLGYSIG
                     VAAKKTTPKTKAKPKAPGTRKRRGVKLKPTELEAKELATVEDSAKLGELAEAVDAAGG
                     AVLARYREPLGGHAVLLAALPLSAVEPTPFQRDVSDAHVLKLTRAMDKTKRFLDPIIT
                     VRHEDKFWTPNGNHRLTALRELGAKAVVALVVPEAKVAYQILALNIEKAHALRERALE
                     VVRMYRELVKAPAESESTYELEFEEPALVTLGFAYEQRGRFAGGAYHPAIRKVDNWIS
                     GDLSDAVHERERRAAALLELDDAVNEAVAKLKEKGLTSPYLKTFVVARVNPLRFIKGT
                     LPSFDELLKTMTKRARGMNVEKIRSEDLARTGGVPDEE"
     gene            4762..5160
                     /locus_tag="VFQ35_26985"
     CDS             4762..5160
                     /locus_tag="VFQ35_26985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934389.1"
                     /translation="MRTFAGIVGLGLGLASTGCQVDMGPNMLLGSLTLEMREPEIVGA
                     LCGRPIDENEIGRRFSTLKVKDIKARRELFGSHGHGTAHVDFQPEHGAACSGTVEYDF
                     SQDSRIVKRYKRSVQHSNTFYYANVVVKHP"
     gene            5273..5914
                     /locus_tag="VFQ35_26990"
     CDS             5273..5914
                     /locus_tag="VFQ35_26990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934390.1"
                     /translation="MELNDPAPSPEPLPTRAVRATFALVAALTLASLIGSALAPVLLL
                     RSPLLLLTLSPDARHVALSAGKVVPAVLLSVVVVRRALFSIGAFGLGVIYGPSGLKWV
                     EARSPRLGRLLRFAERLIARWGAAVLLLVPFASVCMLAGAARLRFVRFIGATLLGHAL
                     WAGSTYYIGTLISDFTDRVLSFLSSNLVESTLVCIALVLLQQVAARRRKTGKN"
     gene            complement(5974..6438)
                     /locus_tag="VFQ35_26995"
     CDS             complement(5974..6438)
                     /locus_tag="VFQ35_26995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934391.1"
                     /translation="MGDVNAGQGGKTMAPEASGGAAGITGSPAGGGGATQFLDQSPEA
                     GPCGQYVYDIEGYCANGAGTVCDFSVAPNCAGIEGTSPPARMYSGCGYVFIYRGLNEG
                     DSETMVYESTTHRLVFYSRVGARASGCSVRGVRVGQQPECDSMTNFCASPMK"
     gene            complement(6958..7458)
                     /gene="rplQ"
                     /locus_tag="VFQ35_27000"
     CDS             complement(6958..7458)
                     /gene="rplQ"
                     /locus_tag="VFQ35_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9934392.1"
                     /translation="MRHKKAGRQFGRNTSHRRAMFRALTANLVAHERIETTDAKAKEL
                     RRVAERLITRAARLGAVAYTPHSDLSPADRARRQAAQQQVARFLRRFAVVTKDGQEQK
                     IDLLEKVFVDLAIRFNGRPGGYTRIIKLGRRRGDNAPMSLIEFVEGNAVPPRAEKAKA
                     APAEEK"
     gene            complement(7462..8487)
                     /locus_tag="VFQ35_27005"
     CDS             complement(7462..8487)
                     /locus_tag="VFQ35_27005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9934393.1"
                     /translation="MVTQIMSRNWRDLIRPKAIQLDFESNSDFYGKFTCEPLERGFGI
                     TIGNSLRRVLLASLQGAAITAVRMDSAVHEFTAIPDVVEDVTDIILNLKEVVFKVESP
                     RTYTVRLEKEGPGVVTAGDIKVVEGLTVLNPDHPIATLDKKGPLSMELTVSVGRGYVP
                     AERNKSPTMPSGTIPIDALFSPIRKVNYTVQNARVGQVTDYDRLVLEVWTNGAVRPVD
                     AVAFAAKILKEQLSIWINFEETEESPYPMPGGDDQPLNENLFRSVDELELSVRSANCL
                     QNANITLIGELVQKTEQDMLKTKNFGRKSLKEIKEILATMNLGLGMKFDNWQGMLERW
                     KQQQQQG"
     gene            complement(8658..9287)
                     /gene="rpsD"
                     /locus_tag="VFQ35_27010"
     CDS             complement(8658..9287)
                     /gene="rpsD"
                     /locus_tag="VFQ35_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9934394.1"
                     /translation="MARYIGPVCKLCRREGNKLYLKGERCFTDKCAVSRRPYPPGQHG
                     QARIKLSEYGLRLREKQKMRRIYGLLEAQFSSYYTTASHLPGRSGEEMLGLIERRLDN
                     VVYRMGFASSRAQARQLVRHSHVLVNGKHVNIPSFRVSPEDKVSIHERSKKIGYIAAA
                     LAAAENRQRPSWVEVDKESLTGTVKTMPVRDELNEPSIREQYVVEYYSR"
     gene            complement(9300..9695)
                     /gene="rpsK"
                     /locus_tag="VFQ35_27015"
     CDS             complement(9300..9695)
                     /gene="rpsK"
                     /locus_tag="VFQ35_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9934395.1"
                     /translation="MATPKARPTQKKVVKKNIFAGIAHIQSTFNNTIVTITDVNGATI
                     SWASAGSRGFKGSRKSTPFAAQLAAEEAGRRAMEHGVRSVAVFVRGPGAGRESALRAL
                     QQVGFKVTLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(9722..10087)
                     /gene="rpsM"
                     /locus_tag="VFQ35_27020"
     CDS             complement(9722..10087)
                     /gene="rpsM"
                     /locus_tag="VFQ35_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9934396.1"
                     /translation="MARISGVDLPRRKHILYALPYIYGIGHATAKRLCAAANVPETKR
                     VEELSEGEVKAIRDAIEANVKVEGDARRDVQANVKRLMDLGCFRGLRHRKGLPVNGQR
                     TRTNARTRKGPRRGAGRRV"
     gene            complement(10100..10213)
                     /gene="rpmJ"
                     /locus_tag="VFQ35_27025"
     CDS             complement(10100..10213)
                     /gene="rpmJ"
                     /locus_tag="VFQ35_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9934397.1"
                     /translation="MKVRPSVKKICDKCKIVKRAGVLRVICNNPRHKQRQG"
     gene            complement(10222..10461)
                     /gene="infA"
                     /locus_tag="VFQ35_27030"
     CDS             complement(10222..10461)
                     /gene="infA"
                     /locus_tag="VFQ35_27030"
                     /inference="COORDINATES: protein motif:HMM:NF013352.4"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9934398.1"
                     /translation="MRSDESERRRATKDETRKGVIEEVMPNALFKVRLEDGRTVSAGA
                     STELRRVVVRLIAGTKVLVELAKHDPNRGKIIQKL"
BASE COUNT         1795 a         3422 c         3375 g         1962 t
ORIGIN      
        1 acagccagcc aatcagaaat tgccatgcgg cgcctctagc agacgaagac tgctcagcgc
       61 gcgagatcga aagtaaggaa gcctcgaaca tggcgctcca accgagggag cgagccgcca
      121 tcaatcaaag atcgggccac atggaagggc gccgtaatcc cgcgaaacgg cgccccgagg
      181 acgcggcaat ttgccacgtc tcgtccaatt tgtgcgcacg cggcagccgc tcccgcgccc
      241 ctctgctgcg atgcagctcg cctccccttt tgctgcgccg cggcctgtca cccgcgccga
      301 ttcagagcgc ggcgcgcgct gcctcttgaa cgcgagcttc ctcttttgca atccaaagtt
      361 tcagcgtgtc gacgtgctga gcttcctctc gcacgaattc gcgggcgacc tttcgaatct
      421 cgacatctgc cgtggtgttc gcgaccgcgt agtagaactc gtaaccgcgc cgctcaccct
      481 gcagcgctgc cttcagcgcg tcgagccgcg agaggcgtgg gtcaccagcc cagagagcgg
      541 tgcgctccgg ggtctcgtga tctggccaag ccgagttcgt cggcagcttc agctcgaccg
      601 agtgggcggc ggttcgagct tttgcctccg catggtgcat gcgtgagtac gtcgccagct
      661 gtcgaaacag cgcggcgacg tcggcgttcc cgcaagcttc catattgttt ccgagttttt
      721 cgaagtggag agcggcgtct tcttcgacct tcacggcgaa gcacaggaag tcgtccaagc
      781 tactgatggt gataccgccc ttgtacacct ccgccgcgat cgagaggtgc ggtccttgct
      841 cggggagggt cgtatcacct tcgaactcga cgaggacgtc ttcgttgcgc acgaaacatt
      901 ggcaggcgag gcgatatcgc ggcggcatgt cgttcacctc cgcgttcatc acctcttcac
      961 gcgtgatctt tccgagctgt ttcaagagct ctttctcttt ttcggtgagc gcgatgccgt
     1021 aagggcgcgc cgaggaaact tgcgtcacct tcaccaaaca cgaaccgcat tcgccatcct
     1081 gacaatcgaa cggaattggg atcttgtgct ctttcgccaa cgcgaggatc gtgccgcgat
     1141 cacccgcgac ggcatacacc gtgacgtcgc gcgacattct gggagatgtg aaagttacgt
     1201 tggccatgcg agtttcggct ccttttgcgc ggcgccctct gcagcctgta tgccaatcgc
     1261 agttgggcac tgcgcgcgag gaaaacggag agcgctccgt cagcaacacg acactcgcag
     1321 gaaaacgctg acgcaggtca ctacggggtg ttcgataaac tacaaagacg acatgccgac
     1381 cgcgcaacat cgcgtttgcg tggctggcct gttctttgct gacgcggcgc acttcgagga
     1441 aagaacgtgg aaaatctgca ggtcttcttg gctcacgcag ttcagctgga gcgcgacgcc
     1501 gcgcggcgct tcgaggatct gatgcactcg atgcacagcg cgggaaacta cgaagtcgag
     1561 gcgttgttcc ggcgactcgg cgagttctcg agatcgcatt tgaaggcggc catggcacgc
     1621 tccggcttcc aggtgttgcc ggtgctcgca ccaggcgaat ttcaatggcc tgaaggaacg
     1681 agcccagaag cggcgggctg gcgcggcgtt gacggcgccc tcgacgtcac gggggcgctc
     1741 gagctcgcgc tcgaaggcga agagtccgga atgcgctttt acgaagcaat cgcgcgtacg
     1801 tcgtcgaacc ctgaagtcac tcacctcgcg cgagagttcg ccgatgaaga gtgggggcac
     1861 gtcaccgagc tccggcgctg gatcgcgagg gcaacggcga acggggccga gttacgctga
     1921 ctcggccctg ctcgtggtgg cggcacgcgc caccctacgc gacctggtga ggcgtactag
     1981 aacgagtggt aaaggggcag ccccgcaggc gcggggaaga acgcgacgtt cgatgcgtag
     2041 ccaatcgtcg agctgctcaa gatcacttcg ggcgtggtcg gagccccggc atccgtcgat
     2101 acgaggagcg cctcgggggc aacgcgcgct acgctacctc gctggtagga gatccagagg
     2161 gcgccggcgt cgtcgaacgc gggccgcgag agcagcgcgg tgacggacaa cgcgatcgtc
     2221 acgctcgaag ggacgctgtg cgcgccaact ccagcgagcg tgctcgcttt cacctcggag
     2281 agcaggttgc ccccgaagtc cacgatccaa agatcgccgt ctgcatcgaa gagcaggttc
     2341 gacgccgcga gatcggcggt gtcggacgca ttgcgcaccg tgaagactgc gtccgctgcg
     2401 gctccggtcg aagcggcgag gcgcgacaag tcgtagcgct tgaccagccc agcgtcggtg
     2461 acccaaaggt ttccggcgcg gtcgaacgcg acgtcgccgt tgtcgtccag gttgctgatg
     2521 attgcgtctg cgctcacctc gccgctcgct gcggccagcg tcgccgcgtc gtagcggagc
     2581 acgacgccgc aagagctcac ccagagcgaa cctttcggcg cgaacgccat cgcgtgaagc
     2641 gtcgtacagg gctgatcggc aagaaccaac tcgcgatccg gaagagccgg gccagaagcc
     2701 gcgaacgcgg tgtggggata gcgcaggagc ggggcttcag cgagcgtagg gccgaaggtc
     2761 cacacgttgc cgtcgcggtc gaaggcgatg tcaccgccgg ctttggcgtc catcgcgagc
     2821 gccggagggc tcgcattgct ggcgccaagc gacgccgccg cgaagccttg aaggttggcg
     2881 tcttcggagc tcacgaccca aagcttgccg ctcgttggaa cggcgctgta gctcaccgtg
     2941 gactgggcgt tcgccggtga tacgcaaaca tcgaccgagc gcggcgctgc ttcgtaaagc
     3001 gagcgaacga ccggcgtcgc gtcggtcacc gccctcgact cgatcgaata gactccgctc
     3061 ggcaaggtgg cgaatgtttt cgaagcggtc gcggtcttcg ctgcaggacc cgtgatcgcg
     3121 atgctcgcgg gaaccgaagg cggtaaccct tcgaccacca aatcgagagc gccagtcagc
     3181 ccggcttcac agttcgcgga gccgcccgaa ccaccccgcg cgcccgaacc gccgcctccg
     3241 cccgcacccg ccgagttgcc gcccgaaccg gaaccgctag caccagcgtg gccggctgcg
     3301 cccgcatccg ttccgccgct gccgtcggac gacgactcga ctgtgcaacc gatgggcgcg
     3361 gcgccaacga gcagagcaaa gagggcggtg agcgagcgag cgtaagtgtg catgcctgta
     3421 actcccagtt gagcgattca gcccaagata cgctgggagg cagcccaacc tcgcattaca
     3481 gcgaattgca ggcggaaatc gcagcgaaat cgcgcgcacg cgcgtcgact cgaatcgagg
     3541 cctacacgag cgctatttct tagttttcac ccgtgacgca tagaaatctg ccggcgtcac
     3601 tcttcgtcgg ggacgccgcc ggtacgcgcc aaatcctcgg aacggatctt ctcgacgttc
     3661 atgccgcgcg cgcgcttggt catcgtcttc aagagctcgt cgaagctcgg caaggttccc
     3721 ttgatgaagc gcagcgggtt cacgcgcgcc acgacgaacg tcttcaagta cgggctcgtg
     3781 agccccttct ctttgagctt cgccactgcc tcgttcaccg cgtcgtcgag ctcgagcaac
     3841 gccgccgcgc gacgttcgcg ctcgtgtacc gcgtcgctca ggtcgcccga aatccaatta
     3901 tccaccttgc gaatcgccgg atggtacgcc ccacctgcaa agcggccgcg ctgctcgtag
     3961 gcaaagccga gcgtcacgag cgctggttct tcgaactcga gctcgtaggt gctctcgctc
     4021 tcggcgggcg ccttcacgag ctcgcggtac atcctcacga cctcgagggc tcgctcgcgg
     4081 agcgcgtgcg ccttctcgat gttgagggcg aggatttgat aagcaacctt ggcctcgggg
     4141 accacgagcg ccaccactgc cttcgcgccg agctcgcgga gcgcggtcag gcgatggttg
     4201 ccgttcggcg tccagaactt gtcctcgtgc cgcaccgtga tgatgggatc gagaaaccgc
     4261 ttcgttttgt ccatcgcgcg cgtcagcttg agcacgtgcg cgtcgctcac gtcgcgctga
     4321 aaaggcgttg gctctaccgc gctcagcgga agcgcagcga ggagcaccgc gtgcccgccg
     4381 agcggctcgc ggtaccgcgc gagaacggcg ccgccggctg catccaccgc ttcggcgagc
     4441 tcgccgagct tcgccgaatc ctcaaccgtc gcgagctcct tcgcctcgag ctcggtgggc
     4501 ttcagcttca ccccgcgtcg cttgcgcgtt ccgggcgctt tcggtttagc cttggtcttt
     4561 ggtgtcgtct tcttggccgc cacgcctatg gagtagccga gcagcgtgcc aaacgcgaac
     4621 cagttcggcg ccgcacgaaa ggcaccgacg gcctcgagtg cgcgccgtcg ctcgtgcgag
     4681 cgccgcggcc gcgcttgcgt gcgtttgtgc cacacaggca aacacgagcg atgtacactc
     4741 gggcccggga ggatgacgaa aatgcgaaca ttcgctggta tcgtgggttt gggtttgggg
     4801 ctggcttcga cggggtgtca ggtcgacatg gggccgaaca tgcttctcgg gagcctgacc
     4861 ttggagatgc gagagcccga gatcgtgggc gcgctctgcg ggcgtccgat tgacgagaac
     4921 gaaatcggtc ggcgcttctc gacgctgaag gtcaaggaca tcaaggcgag gcgcgagctc
     4981 ttcggcagcc acggacacgg caccgctcac gtcgatttcc agcccgagca cggcgctgcg
     5041 tgtagcggca ccgtcgaata cgacttcagc caagactcac gcatcgtgaa acgctacaag
     5101 cgcagcgtgc agcactcgaa taccttctat tacgccaacg tcgtcgtgaa gcatccttag
     5161 gcgtgcgagc caacggcggc gatgggggct gcgcgcgacc cgccgcgctc gccgctccgt
     5221 gagaaatcat ccagggcttg cgcggactcc aggcctggcg caccgtcgag aggtggagct
     5281 caacgaccct gcgccgagcc cggagccgct tcccacgcgg gcggtgcgcg cgacgtttgc
     5341 gctcgtcgcc gcgctgacgc tcgcgagcct gatcgggagc gcgctcgcac ccgtgctcct
     5401 ccttcggtcg ccgttgctgc tgctcacgct ctcgcccgat gctcgtcacg tcgcgctctc
     5461 ggcgggaaag gtcgtgccgg ccgtgttgct cagcgtcgtg gtcgtgcggc gcgcactctt
     5521 cagcatcggc gccttcggcc tcggcgtcat ctacggccct tcgggcttga agtgggtgga
     5581 agctcgctcg ccgcgcctcg gtaggctcct gcgctttgcg gagcgactca tcgcgcgttg
     5641 gggcgcggcg gtgctgcttt tggtcccgtt cgcgagcgtg tgcatgctgg cgggcgcggc
     5701 gcggctgcgc ttcgtccgct ttatcggggc cacgctgctc ggccatgcac tttgggcggg
     5761 gtcgacctat tacatcggca cgttgatctc cgacttcacg gatcgcgtgc tctcgttcct
     5821 ctcgtcgaac ctcgtcgaga gcacgctcgt gtgcatcgcg ctcgttctcc tgcagcaagt
     5881 cgcggcgcgc cgacgaaaga ccggcaagaa ctgaaccgcg gcgcctgagg tccactatca
     5941 cgccggcgag gcgcgacgga cggcagcggc ggttcacttc atcgggctgg cgcaaaagtt
     6001 cgtcatgctg tcgcactcgg gctgctgacc aactcgaaca ccgcgaacgc tgcagccaga
     6061 cgcgcgcgct ccaaccctcg aatagaacac gagccgatgc gtcgtgctct cgtacaccat
     6121 cgtttcggag tcgccttcgt tgagcccgcg gtagatgaag acgtagccgc agccggaata
     6181 catacgtgcc ggcggtgaag tcccttcgat tccagcgcag ttcggcgcaa ccgagaagtc
     6241 gcacaccgtc cccgcaccgt tcgcgcagta accctcgata tcgtaaacat actgcccgca
     6301 cggacctgct tcagggctct ggtcgagaaa ctgtgtcgcc ccgccaccac ccgctggcga
     6361 ccccgtgatt cccgccgcgc cgccactcgc ttcgggcgcc atcgtcttcc caccttgccc
     6421 tgcgttgacg tctcccatcc cacctgtcgc cccggtaggc gagccatccg aggagccgcc
     6481 acaggcggtg agcgcaaacc ctaaaagcac agacgcagcg caacgagcca ttgagctttt
     6541 cacgaacgct gctgacgcaa gggcgatgcc agagcccaaa gccccgtatt cggctcgaat
     6601 cgcatgcact gtccgttcaa cggacctgcc gctagctcaa ctcgacacgg ggcggcacag
     6661 attggcaaac gtatcccaga gaacgtccgc cgcgctcctg cctcaagcag aaagccaacg
     6721 gcaccgttga ccgagctctc ggcgcacggc gtacccaggc ccagcgagat caagcgtgcg
     6781 cacctgggcg gacgatgtcg acttgtcgtg ctcgccatcg gcagtgagcc aacaaaggtt
     6841 cgcgcaatgg gcgagcgaac ccgacaaaac gcgaacagcg cggcacctct agatagagct
     6901 accacgctgt cgctgacgcg gtgccaccag ggcgacgccg ctcgtagccg aattgggcta
     6961 cttctcttcg gccggggcag ccttggcctt ttcggcgcga ggcggcacgg cgttgccttc
     7021 gacgaactcg atcagggaca tcggggcgtt gtcgccacga cgacggccga gcttgatgat
     7081 gcgggtgtag ccaccgggac gaccgttgaa acggatcgcg aggtcgacga acaccttctc
     7141 gagcaggtcg atcttctgct cttggccgtc cttcgtcacg accgcgaagc ggcggaggaa
     7201 gcgagcgacc tgctgctgag cagcttgacg acgcgcgcga tcagcggggc tgaggtcgct
     7261 gtgcggcgtg taagccacgg ctccgagccg cgcggcgcgc gtgatgagac gctcggcgac
     7321 acgacgaagt tccttcgcct tggcgtcggt cgtctcgatg cgctcgtgcg ccacgagatt
     7381 cgcggtgagc gcgcggaaca tcgcgcgacg gtggctggtg ttacggccga attgtcggcc
     7441 tgcctttttg tgcctcatgg ttcagccttg ttgctgctgc tgcttccagc gctcgagcat
     7501 gccctgccag ttgtcgaact tcatcccaag gccgaggttc atcgttgcga ggatctcttt
     7561 gatctccttc aacgacttgc ggccgaagtt cttggtcttc agcatgtctt gctcggtctt
     7621 ctgcacgagc tcaccgatga gcgtgatgtt ggcgttctgc aagcagttcg ccgagcgcac
     7681 gctgagctcg agctcgtcca ccgagcggaa aaggttctcg ttgagcggct ggtcgtcgcc
     7741 gcccggcatc ggatacgggc tctcctccgt ctcctcgaag ttgatccaga tggagagctg
     7801 ctccttcagg atcttcgcgg cgaaggccac cgcgtcgacg gggcgcaccg cgccgttggt
     7861 ccacacctcg aggacgaggc ggtcgtagtc ggtgacctga ccgacgcgcg cgttctgcac
     7921 ggtgtagttc accttgcgga tcggcgagaa gagcgcatcg atcgggatcg tgccgctcgg
     7981 catcgtcggg ctcttgttgc gctcggcggg cacgtagccg cggccgacgc tgaccgtgag
     8041 ctccatcgaa agcggaccct tcttgtcgag ggtcgcgatc gggtgatccg ggttcaacac
     8101 cgtgaggcct tcgacgacct tgatgtcgcc cgcggtgacg acgccggggc cttccttctc
     8161 gaggcgcacg gtgtaggtgc ggggcgactc gaccttgaac accacctctt tgaggttgag
     8221 gatgatgtcg gtcacgtcct cgacgacgtc ggggatggcg gtgaactcgt gcacggcgct
     8281 gtccatgcgg acggccgtga tggcggcgcc ctgcagcgac gcgagcagca cgcggcgcag
     8341 cgagttgccg atggtgatcc cgaagccgcg ctcgagaggc tcgcaggtga acttcccgta
     8401 gaaatcgctg ttcgactcga agtcgagctg gatcgccttc gggcggatca gatcgcgcca
     8461 gttacggctc atgatttgtg tgaccatgcg ttacctctct tttggcttgg ggttcgcgcg
     8521 gccgtcccga gtcgtgaggg aacccgctcc actcgacgac tccgcggcct gcccgcgttg
     8581 agaaggacgt cccagggacg ccctcctcca aacgaatcct actgaagcca cagcggaaac
     8641 ccgccgcggc gcggcgacta gcgagagtag tactcgacga cgtactgctc acgaatcgac
     8701 ggctcgttga gctcgtcacg caccggcatc gtcttgacgg taccagtgag ggactccttg
     8761 tcgacctcga cccagctcgg gcgctgacgg ttctccgccg cggccaaggc cgccgcgatg
     8821 taaccgatct tcttgctgcg ctcgtggatc gagaccttgt cctccgggct cacccggaag
     8881 ctcgggatgt tgacgtgctt gccgttcacg agcacgtggc tgtgacggac gagctgacgc
     8941 gcctgagcgc ggctcgaagc gaagcccatg cggtaaacga cgttgtcgag gcggcgctcg
     9001 atgaggccga gcatctcttc accggagcgg ccggggaggt gcgaggcggt ggtgtagtag
     9061 ctcgagaact gcgcctccaa gaggccgtag atacggcgca tcttctgctt ttcacgcaga
     9121 cggagaccgt actcgctgag cttgatgcgc gcctggccgt gctggcctgg cggatagggg
     9181 cgccgcgaaa cagcgcactt gtccgtgaag caacgctcac ccttgaggta gagcttgttg
     9241 ccttcacgac ggcagagttt gcaaaccgga ccgatatagc gagccatccg aatactccgt
     9301 caaacgcgac gccgcttggg tggccggcat ccgttgtggg ggatgggggt cacgtcgcgg
     9361 atcagcgtga ccttgaagcc aacttgctgc agagcgcgaa gtgcgctctc gcgccccgcg
     9421 ccaggacctc gaacgaacac ggcaaccgag cgcacgccgt gctccatcgc gcgacgacct
     9481 gcctcttcgg cggcgagctg cgccgcgaac ggcgtgctct tgcgcgaacc cttgaaaccg
     9541 cgcgacccag cgctcgccca cgagatggtg gcgccgttca cgtccgtgat ggtcacgatg
     9601 gtgttgttga aggtggactg aatgtgcgcg atgccggcga agatattctt cttcaccact
     9661 ttcttctggg tcgggcgcgc tttcggggtt gccatgtgtt cctcagtatt ccttcaacag
     9721 atcagacgcg gcgtccggcg ccacggcgcg gacccttgcg cgtacgcgcg ttggtgcggg
     9781 tgcgctgacc gttgaccggc agccccttgc ggtgacggag gccgcggaaa caaccgaggt
     9841 ccatcaagcg cttgacgttg gcctgcacgt cgcggcgggc gtcgccttcg accttcacgt
     9901 tcgcttcgat cgcgtcacgg atggccttga cctcgccttc gctcaactcc tcgacgcgct
     9961 tcgtctcagg aacattggcg gcggcgcaca ggcgcttggc cgtggcgtga ccgatcccgt
    10021 agatgtacgg gagcgcgtaa aggatgtgct ttctacgggg aagatcgacg ccggagatgc
    10081 gtgccatagt ttcttactct cagccctgac gctgcttgtg gcgggggtta ttgcagatca
    10141 cgcgcaggac gccggcgcgc ttcacgatct tgcacttgtc gcagattttc ttgacgctgg
    10201 gtcgcacctt catgtcctgc ctcacaactt ctgaatgatc ttgccccggt tggggtcgtg
    10261 tttggcgagc tctacgagga ccttggttcc agctatgagc cggaccacca cgcgccgaag
    10321 ttccgtcgat gcccctgcgc tgaccgtgcg tccgtcttcc agccgcactt tgaaaagcgc
    10381 attgggcatt acttcttcga tcacgccctt tcgggtttca tccttcgttg ctcgcctccg
    10441 ttcactctcg tccgagcgca cgcaagatgc gctccgtgat ctcttggggg gttcccactc
    10501 cgtgcacctg acagagcagg ttcaggtccg cgtagtgcgg tcccagcgcg gcgg
//
LOCUS       DASTKS010001264         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1767028, whole genome shotgun sequence.
ACCESSION   DASTKS010001264 DASTKS010000000
VERSION     DASTKS010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767028"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFQ35_27035"
     CDS             <1..537
                     /locus_tag="VFQ35_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9934399.1"
                     /translation="VARQRGVRIRRPEELCRGAHIFGGAEVRVLAPCPSFVPGRGAND
                     NSIVLRVGFGERSLLLTGDAEHLAETELLRNHPQELRADVLKVGHHGSRTSSSPELLE
                     AVRPSVATISCGVRNRFGHPVPEVLARISGYGAQVFRTDRDGAVELTTDGASLEVASS
                     YGDTALALGYRLAALDGD"
     gene            570..1103
                     /gene="hpt"
                     /locus_tag="VFQ35_27040"
     CDS             570..1103
                     /gene="hpt"
                     /locus_tag="VFQ35_27040"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9934400.1"
                     /translation="MTKSEKIVPLLTESQIAGRVAELGRAISADYGDQEVVLVCVLKG
                     SFVFAADLARHMTCPLRVEFFGVKSYGAETSSSGVVQITLDLAHPIEGRHVLIVEDII
                     DTGLTLSYLSEQLRARGPASVKLAALLHKPARKRREVQIDYLGFTIEDVFVVGYGLDH
                     AERHRNLPYIGVVEKAE"
     gene            1206..1592
                     /locus_tag="VFQ35_27045"
     CDS             1206..1592
                     /locus_tag="VFQ35_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934401.1"
                     /translation="MKAKGSLRNSHSAVLLALACLAGVACGGRTEGGETDPTDSSAGG
                     ASNATPGSGGATSGQGSNGSGFGSTTLPPCVLGVEQWLADACDYYAEGRCYEKKLEAC
                     ACVCPRNVANTTCLSSFDVPARVTCF"
     gene            1862..>2376
                     /locus_tag="VFQ35_27050"
     CDS             1862..>2376
                     /locus_tag="VFQ35_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934402.1"
                     /translation="MMRACIRAIGSLSVFSLVAACGSSAGNLFDSNGGGAGAPMGGSS
                     ATGGGTGTALGGSPGEVSGGVTSTGGAAVGGGAEASGGNPVIGTGGASNGVGGKASGA
                     GGTGGAAASGGASSAGAAPTSGGALASGGASSEGGAPAEGGTSTSGGAMTTGGSAMGG
                     TLAAGGVQSSGG"
BASE COUNT          368 a          736 c          854 g          418 t
ORIGIN      
        1 gtcgctcgcc agcgaggcgt gcgcattcga cggccagaag agctctgccg tggtgcccac
       61 attttcgggg gtgcagaggt gcgcgtgctc gcgccgtgcc cttcgttcgt cccgggacgc
      121 ggcgccaacg acaattcgat cgtgctgagg gtcggcttcg gcgagcgctc gctgctgctc
      181 acgggcgacg ccgagcacct cgccgagacc gagctcttgc ggaaccatcc gcaggagttg
      241 cgcgccgacg tgctgaaggt cggccatcac gggagccgca cgtcgagcag tccggagttg
      301 ctcgaggccg tgaggccgag cgtcgccacc atctcgtgcg gcgttcggaa tcgcttcggt
      361 catcccgttc ccgaagtcct ggcgcgcatc tctgggtacg gggcccaggt tttccgcacg
      421 gatcgcgacg gcgccgtcga gctcacgacc gacggtgcct ccctcgaggt ggcttcttcg
      481 tacggagaca ccgcgctcgc gctcgggtat cggctcgccg cgctcgacgg ggattgacgc
      541 ggatgtggtc gccggcattg gattctttca tgacgaagtc ggagaagatc gtcccgctcc
      601 tcaccgaatc gcagatcgcc gggcgcgtcg ccgagctcgg gcgcgccatc agcgccgact
      661 acggagacca agaggtcgtc ttggtgtgcg tattgaaagg gagcttcgtc ttcgcggccg
      721 acctcgcgcg gcacatgacc tgcccgctgc gcgtcgaatt tttcggggtc aagagctacg
      781 gggccgagac gagctcgagc ggagtcgtac aaatcaccct ggatttggcg caccccatcg
      841 aggggcggca cgtgctcatc gtcgaggaca tcatcgatac ggggctgacg ctgtcgtatc
      901 tgagcgagca gcttcgggcg cgcgggccag ccagcgtcaa attggcggcg ctgctccaca
      961 agcccgctcg caagcggcgc gaggtgcaga tcgactacct cggcttcacg atcgaagacg
     1021 tattcgtggt cgggtacggc ttggatcacg ccgagcggca ccggaatctt ccgtacatcg
     1081 gggtggtcga gaaggccgag tgagcgccaa ctgaggtggc ggggcgccgc gcgatatccg
     1141 aggaatatga gcggtctgcg gcttgctaca cttcgcctgg gcaatcggcg cgcgcgagca
     1201 agaccatgaa agccaaaggt tcgctgcgaa actcacattc agccgtgctt ctggccctgg
     1261 catgtctcgc gggagtcgcg tgcggcggca ggactgaagg cggcgaaacg gatccgacgg
     1321 attcttcggc tggcggcgct tcgaacgcga cgcctgggtc cggcggtgcg acgagcggtc
     1381 agggctcgaa cggtagcggc ttcggctcca cgacccttcc cccctgtgtt ctcggtgtgg
     1441 agcagtggct cgccgacgcg tgcgactact acgccgaggg ccggtgctac gagaagaagc
     1501 tcgaagcgtg cgcgtgcgtc tgtccgcgta acgtggcgaa cacgacttgt ttgagctcgt
     1561 tcgacgtgcc cgcgcgcgtt acatgcttct aggcagcgtc ccgatcgaaa gctcgctgtg
     1621 tgtgggcaac gctgcgcgct ttctctcggc tcgttgaaag attctccgat acatcgagcg
     1681 cgtcacacga tgctggcgag cgggcgccaa ttggctattc tgatgccttc ttccgaaacg
     1741 gattcggaag agactgctcg cgcttgccac cagcgcggag tcaaatcttc ggccagtccc
     1801 caagcgttca tcgcttcaac gccgaatctc tcccgcgagg attcggagca ctctgcgatt
     1861 catgatgcga gcctgcattc gcgcgattgg tagcctcagc gtgttctccc tcgtcgctgc
     1921 gtgtggcagc agcgcgggga acctcttcga ttcgaacggc ggcggcgctg gtgcgcccat
     1981 gggcgggagc tcggcgaccg ggggcgggac gggcactgcg ctcggcggat cgcccggcga
     2041 agtgagtggc ggggttacgt cgactggtgg cgcggcagtg ggcggcggag ccgaagcgag
     2101 cggcggcaat cccgtcatcg gtacgggtgg cgcgtcgaac ggagtcggtg gcaaagcgtc
     2161 cggtgctggc ggcactggcg gcgctgccgc gagtggcggt gcttcgtcgg cgggcgcggc
     2221 gcctacgagc ggtggcgcgt tggcgagcgg cggcgcctcc agcgaaggcg gcgctcctgc
     2281 tgaaggcggc acttcgacga gcggcggcgc tatgacgacg ggcggcagcg cgatgggcgg
     2341 aacgctggca gccggcggcg tgcagtcgag cggcgg
//
LOCUS       DASTKS010001265         4245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_425967, whole genome shotgun sequence.
ACCESSION   DASTKS010001265 DASTKS010000000
VERSION     DASTKS010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425967"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VFQ35_27055"
     CDS             <1..1543
                     /locus_tag="VFQ35_27055"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9934403.1"
                     /translation="QAIGPSPLKRLTRFEYANSVRDIFGIDLKTEDLFPRDEQALNFD
                     NQAGTLSLTDLHVQGYLDAGDIVADWLARDPSRLEAVAGCGLEGAECARQLVTSLGRR
                     LERRALSDAEVSSLLELFGDDYTAAGFSEGASRVVAALLQHPEFVYRVERPAADVGSR
                     ELASPWVLASRLSFLFWGSGPDDALLDAAAQGSLATKADVAREARRLLADPRARRGIL
                     HFYMQWLDLGSFDDVEKDARLFKYWDDTLRSDLKNETARFLEGVLWEDDARFSTLLTA
                     PYTFANAALADFYGIPPAGGSSDELVKTSFPNASQRSGVLTQASILSRQAKANQSDPI
                     HRGKFIRERFFCAPPPPPPPDLVVSPPKLDPRKTTRERFADHRANAACAGCHDLLDPV
                     GFVFENYDAIGRFRTTEADAPIDASGYLKNTDIPGTVTGVPELAAKLSTSKEVRSCMI
                     KQWFHFAFARGEGELDACTLQKLEAVFDQTHGNLAELLVALTQTDPFLYVPPAPAAEA
                     DEDGQ"
     gene            1587..2903
                     /locus_tag="VFQ35_27060"
     CDS             1587..2903
                     /locus_tag="VFQ35_27060"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9934404.1"
                     /translation="MTTSRRNVLRALLASTIGLPWLETFAERSAHAQALPNKRFIAMF
                     SGNGTIRKAWLPAGGESAFTLSPILEPLAPHQSDIVVVSGVDQQGAGGDGHQNGIGGM
                     LTGAPLLPGRFAGVGAPPAGWADGPSVDQRIAQVISRGLPFQSLELAVQPGSADIWGR
                     MIYRARNQPLPPREDPVKVFDDLFGAAALAPADRERRRARRQSILDYVSRQVTELSGR
                     VAAADKQRLDAHLTNLRTLEQRLDSQASALSACQVPAPPAAATSSNDAYPAIGDAMID
                     LLVLALACGQTRVASLQWSRSVSNVRFTWLGIDDEHHGLSHAPDSDTTAQQKLIKINR
                     WYAERYAALIAKLKSYPEGDRTLFDNCLLLWCNELGTGNTHSRDNAPYVLAGSAGGAL
                     ATGRYLNFDGVVPHNNLLLSILKIMGLSDTSFGKAEWCTGYLPGLA"
     gene            2924..3721
                     /locus_tag="VFQ35_27065"
     CDS             2924..3721
                     /locus_tag="VFQ35_27065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934405.1"
                     /translation="MLQSMVYRWHWSVALGALLSFACSGNATDDSNAGGAGGAGGNGA
                     GAGGGLSQAVCDQAANFAPINPTGLFDDMEDGNGVVAYVGGRNGSWWLSTDGTDGTTT
                     PEANQAPTPETIVGGRCGSKKAIRVTGQGFTDWGAVLSAGMAYSSHADPVDLSSFTGL
                     RFWARVGEQNTSGIRAQFQDAQTNPEGGQCVDSPGNPEACWDGFGTALSPIDTQWRMY
                     ELKFSQMAQRGFGHQGSALDAAHVYGLEFNLEPNSVFDLWVDDLWFF"
     gene            3918..>4245
                     /locus_tag="VFQ35_27070"
     CDS             3918..>4245
                     /locus_tag="VFQ35_27070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934406.1"
                     /translation="MPAAPSKAGSIDTGLWHQFTLQARRYIAGTLLLAAYQFAQYEFD
                     TCLRKAINEALEHQRDAATHSGVVLVVVASLGLIARVLSRMAIFNAGRVAEYRLRRSL
                     SEKLLLL"
BASE COUNT          728 a         1433 c         1342 g          742 t
ORIGIN      
        1 tcaggcgatc ggcccgtcgc cactcaagcg actcactcgc ttcgaatacg cgaactccgt
       61 acgcgatatt ttcggcatcg acctgaagac ggaggacttg ttcccgcgcg acgaacaagc
      121 gctgaacttc gataaccaag ccggcacttt gagcctcact gacctgcacg tgcagggcta
      181 cctcgacgcc ggagacatcg tcgcggattg gctcgcgcgc gatccctcgc gcttggaagc
      241 ggttgcggga tgcggcctcg agggtgccga gtgtgctcgc cagctcgtca cgtcgctcgg
      301 acgacgcttg gagcggcgcg cgctcagcga cgccgaagtg agctcactgc tcgagctctt
      361 tggagacgac tacactgccg cgggcttctc cgaaggcgcg tcgcgcgtcg tggccgcgct
      421 gctgcaacat cccgagttcg tgtatcgcgt ggagcgacca gcggcggacg tcgggtcgcg
      481 cgagctcgcg tcgccctggg tgctcgcttc tcggctctcg tttctatttt ggggttccgg
      541 acccgatgac gcgctgctcg acgccgctgc gcaggggtcg ctcgcgacca aggcggacgt
      601 cgcccgagaa gcgcggcgtt tgctcgccga tccgcgcgcg cggcgaggga ttttacattt
      661 ctacatgcag tggctcgacc tcggcagctt cgacgacgtc gagaaggacg cccggctctt
      721 caagtactgg gacgacacgc tgcggagcga cctcaagaac gagacggcgc gcttcctcga
      781 gggcgtgctc tgggaagacg acgcgcgctt ctccaccttg ctcaccgccc cgtacacgtt
      841 cgcgaacgcg gcgctcgccg acttctacgg aattccgccc gcaggcggga gctccgacga
      901 gctcgtgaag acgtcgttcc ccaacgcttc gcagcgcagc ggcgtgctga ctcaagcgtc
      961 gattctgtcg cgccaagcca aggcgaacca aagcgatccg atccatcgcg gtaagttcat
     1021 ccgcgagcgc ttcttctgtg cgcccccgcc acctccgccg cccgacttgg tcgtatcccc
     1081 accgaagctc gacccgcgca agaccacgcg ggagcgcttc gcggaccatc gagcgaacgc
     1141 cgcgtgcgcc ggctgccacg atttgctcga tccggtcggt ttcgtattcg aaaactacga
     1201 cgcgatcggg cgctttcgca ccaccgaggc cgacgcgccc atcgacgcct cgggctatct
     1261 caagaacacc gacatccctg gcaccgttac cggtgtgccc gagctcgcgg ccaagctctc
     1321 gaccagcaaa gaagtgcgaa gctgcatgat caagcagtgg ttccactttg cgttcgctcg
     1381 cggtgaaggc gagctcgacg cttgtactct ccaaaagctc gaagccgtct tcgaccaaac
     1441 ccacggtaac ctcgccgagc tcctcgtcgc gctcacccaa acggaccctt tcctctacgt
     1501 accgcccgcc cccgccgccg aagccgacga ggacggccag tgaaagtcca tcgcgcatca
     1561 cctcgtgaag aaggagcgtc tctgtcatga ctacgtcgcg ccgtaacgtg ctgcgggcgc
     1621 tgctcgcttc caccattggg cttccctggc tcgagacgtt cgcggagagg tcggcgcacg
     1681 cgcaggcgct ccccaacaag cgcttcatcg cgatgttctc cgggaacggg accatccgca
     1741 aagcgtggtt gccggcggga ggcgagagcg cgttcacgct gtcgccgatt ttggagccgc
     1801 tcgcaccgca ccagagcgac atcgtcgtgg tgtcgggcgt cgaccagcaa ggagccggcg
     1861 gcgatggcca tcaaaatggc atcggaggca tgctcaccgg tgcaccgcta cttccaggtc
     1921 gcttcgcggg cgtcggtgcg ccaccggcgg gctgggcaga tggcccatcg gtggaccagc
     1981 gcattgctca ggtgatctcg cgcggactgc cgtttcaatc gctcgagctc gcggtgcagc
     2041 ccggctcggc cgacatctgg gggcgcatga tctaccgcgc gcgcaaccag ccgctcccgc
     2101 cgcgcgagga tcccgtaaag gtgttcgacg acttgttcgg cgcggccgcc ctcgcgcccg
     2161 cggatcggga gcgccgtcgt gcgcggcggc agtcgatcct cgattacgtc agccgtcaag
     2221 tgacggagct ctcgggtcgc gtcgctgcag ccgacaagca gcgcctcgac gcgcacctca
     2281 cgaacctgcg cacgctcgag cagcgcctcg actcgcaggc gagcgcgctt tccgcttgcc
     2341 aggtgccagc tccgcccgcg gccgcgacca gcagcaacga cgcgtatcct gcgatcggcg
     2401 acgcgatgat cgatctgctc gtgctcgcgc tggcttgtgg gcaaacgcgc gtcgcatcgc
     2461 tgcagtggtc gcgctcggtt tccaacgtgc gtttcacttg gctcggcatc gacgacgagc
     2521 accacggctt gtcacacgcc ccggacagcg acaccaccgc gcagcagaag ctcatcaaga
     2581 tcaatcgctg gtacgcggag cgctacgccg cgctcatcgc caagctcaag agctaccccg
     2641 aaggcgaccg cacgctgttc gacaattgcc ttttgctctg gtgtaacgag ctcggtaccg
     2701 gtaacacgca tagccgcgac aacgctccct acgtgctcgc cgggagcgcc ggcggtgcgc
     2761 tcgccacggg gcgttacctg aacttcgacg gcgtcgtccc tcacaacaac ttgttgctct
     2821 cgatcctgaa aatcatgggg ctctccgata ctagcttcgg taaggcagag tggtgcacgg
     2881 gctacttgcc cgggctcgcg tgagaaatat gaaaggcgca agaatgctgc aatcgatggt
     2941 ttatcgctgg cattggtccg tcgcgctcgg cgcgctgctc tcgttcgcat gctccggcaa
     3001 cgcgacggac gattcgaatg ccggtggcgc aggtggcgct ggcggcaacg gcgcgggcgc
     3061 cggcggcggc ctttctcaag ccgtgtgcga ccaagccgcg aacttcgcgc cgataaaccc
     3121 caccgggctc ttcgacgaca tggaggacgg caacggcgtc gttgcgtacg tcggtggtcg
     3181 caacggaagc tggtggctct ccaccgacgg aaccgatgga accacgacgc cggaggcgaa
     3241 tcaggcgccg acaccagaga ccatcgtggg cggccgctgc ggaagcaaga aagcgatccg
     3301 cgttaccggt cagggcttca ccgattgggg ggcagtgctg agcgccggaa tggcgtactc
     3361 ctctcacgcc gatcccgtcg atttgagcag ctttaccggt ttgcgctttt gggcgcgcgt
     3421 cggcgagcaa aacaccagcg gcatccgcgc gcagttccag gacgcccaaa ccaacccaga
     3481 gggtgggcag tgcgttgatt cgcctgggaa cccggaggcg tgctgggatg ggttcggtac
     3541 cgcgctcagt cccatcgaca cgcagtggcg catgtacgag ctcaagttct cgcaaatggc
     3601 gcagcgcggc ttcggtcacc aggggtcggc gctcgatgca gctcacgtct acggcctgga
     3661 gttcaacctc gagccgaact cggtcttcga tctatgggtc gacgaccttt ggttcttctg
     3721 agagcgtcgg caaccgcgac ggaagcgccg cgtcagaggc ggcggaccta catctggacg
     3781 gggtgaatcg tcggagcgcg ccggccgaac cgggcggcgg atttcaagct gggccgagtg
     3841 gccgatggta cgggtgagcg ccttttgaag caaaccccct cgacgttcga gagaaggacc
     3901 gtagattctc ggccactatg cccgcggcgc cgtccaaggc cggcagcatc gacaccggcc
     3961 tttggcacca attcaccctg caagcgcgcc gctacatcgc cggcacgctg ctcctagccg
     4021 cgtatcaatt cgctcaatac gagttcgaca cgtgtctgcg gaaggccatc aacgaagcgc
     4081 tcgagcacca gcgcgacgcc gcgactcact ccggcgtcgt actcgtcgtg gttgcgtcgc
     4141 tcgggctcat cgcgcgcgtg ctctcgcgca tggcgatttt caatgcgggc cgcgtcgcag
     4201 agtaccggtt gcgtcgctcg ctttcggaaa agctcttgct cttgg
//
LOCUS       DASTKS010001266         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1992682, whole genome shotgun sequence.
ACCESSION   DASTKS010001266 DASTKS010000000
VERSION     DASTKS010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992682"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /gene="istA"
                     /locus_tag="VFQ35_27075"
     CDS             <1..1387
                     /gene="istA"
                     /locus_tag="VFQ35_27075"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9934407.1"
                     /translation="NRFATARFQKRALALDPYREFVRQTLEQYPRLRSTRLFEMIRAR
                     GYEGSVFPLRRYVRRIRPVPQHEAFFRLSTLPGEEAQVDWGSFGKLRVGNAERQLSCF
                     VLVLSWSRATFARFTLDQTLESFLRCHVEAFTRFGGVPRTILYDNLKSVVLERQGDLF
                     RFHNRILELAGHYHFAPKPVAVARGNEKGRVERRIRDLRESFFAARTFGSLADLNRQL
                     DTWLEHVAHQRRVPGDSEKRVSEALDEERSRLLPLPEHPFETDTIRAICSGKSPYLRF
                     DKNDYSIPHALVRKPLTLVASDSLVRVLDGQTEVARHRRSWSAREQIEDEGHLAALAA
                     AKHKAREHRGRNRLFAACSAAKRFLAEVALHGGHLGGTTSRLLRLLEQYGPAELDIAL
                     ADAHQRKAFAAQSVAHVLDQRRRARGAPVPLDMPLPRDPRVQGLVVTQHSLERYDRLN
                     GSDPAEKQVKP"
     gene            1384..2175
                     /gene="istB"
                     /locus_tag="VFQ35_27080"
     CDS             1384..2175
                     /gene="istB"
                     /locus_tag="VFQ35_27080"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9934408.1"
                     /translation="MTERDLRALLRTLGLTATADALDDLIALATKKRFGPVELFQHVA
                     ILEEKERARRGLERRIKRSRLERFKPMADFDWNWPTKIERALVESVLRLDFLEEPRNV
                     VLAAPQGLGKTMIAQNVTHNAALAGHGVLFISAAQLLLDLGAQESARALERRLKYYAK
                     IGLLVLDEIGFLAFDNRNADLLFQVVSRRYEKKSTVLTTNLAFRDWPTIFPNATCATA
                     LIDRVVHHADVIAIEGESYRAREAELHAKDRRSRRREKPEPEPAA"
BASE COUNT          389 a          880 c          663 g          394 t
ORIGIN      
        1 caaccgcttc gcgacggcgc gctttcagaa acgcgccctc gccctggatc cgtaccgcga
       61 gttcgtgcgg cagacgctcg agcagtaccc tcggctgcgc tcgacgcggc tgttcgagat
      121 gattcgcgct cgaggctacg agggcagtgt ctttccgctc cggcgctatg tccgcaggat
      181 ccggcctgtg ccccagcatg aggcgttttt ccggctcagc acgctgccgg gcgaagaggc
      241 ccaggtcgat tggggctcgt tcggaaagct tcgcgtcggt aacgccgagc ggcagctttc
      301 ctgcttcgtg ctcgtgcttt cgtggtctcg cgccaccttc gcgcggttca cgctcgatca
      361 gaccctcgag agctttctcc gctgccacgt cgaggcgttt actcgcttcg ggggcgtccc
      421 gaggaccatc ctctacgaca acctgaaaag cgtcgttctc gagcggcagg gcgacctctt
      481 ccgctttcac aaccggatcc tcgagctcgc cggtcattac cacttcgcac ccaagcccgt
      541 cgccgtcgcc cgcggcaacg agaagggtcg cgtcgagcgg cgcatccggg atctcagaga
      601 atcgttcttc gccgcccgca ccttcggctc gctcgccgat ctcaatcgcc agctcgacac
      661 gtggctcgag catgtcgctc accagcggcg tgttccaggc gactccgaga agcgcgtctc
      721 cgaggctctc gacgaagagc gatcgcgcct cttgccgttg cccgagcatc cgttcgagac
      781 ggacaccatt cgtgcgattt gctcggggaa atccccatac ctgcgcttcg acaagaatga
      841 ttactccatc ccgcacgcac tcgtccgaaa accgctcacg ctcgtcgcct ccgactcgct
      901 ggtgcgcgtg ctcgacggcc agaccgaggt cgctcgtcac cgccggtcct ggagcgctcg
      961 cgagcaaatc gaggacgaag ggcacctcgc cgcccttgcc gccgccaaac acaaggcccg
     1021 cgagcaccgc ggacgcaatc ggctctttgc cgcttgttcc gccgccaagc ggttcctcgc
     1081 cgaagtcgct ctccacggcg gccacctcgg cggaacgacc agccgcttgc tccgcctcct
     1141 cgagcagtac ggcccggccg agctcgacat cgcgctcgcc gatgcgcacc agcgcaaggc
     1201 cttcgccgct cagtccgtcg ctcacgtcct cgatcagcgc cgtcgcgctc gcggcgcccc
     1261 cgttccgctc gacatgccgc tcccgcgcga cccgcgcgtc caaggcctcg tcgtcactca
     1321 gcactcgctc gagcgttacg accggctcaa cggctcggat cccgcagaaa agcaggtgaa
     1381 accgtgaccg agcgtgacct ccgggccttg ctccgtaccc tcgggctcac cgctaccgcc
     1441 gacgcgctcg acgacctcat cgctctcgcc accaagaagc gcttcggtcc cgtcgagctg
     1501 ttccagcacg tcgctatcct cgaggagaaa gagcgcgctc gccgcgggct cgagcgtcgc
     1561 atcaaacgta gccgcctcga gcgcttcaaa cccatggccg acttcgactg gaactggccg
     1621 accaaaatcg aacgagccct cgtcgagtcc gtactgcgtc tcgacttcct cgaagagccg
     1681 agaaacgtcg tgctcgccgc gcctcagggc ttgggcaaaa ccatgatcgc gcaaaacgtc
     1741 acgcacaacg ctgcgcttgc gggccacggc gtcctcttca tctccgccgc ccagctcctg
     1801 ctcgatctcg gtgctcagga gtcggctcgc gccctcgagc gcaggctcaa gtactacgct
     1861 aaaatcggcc tgctcgtgct cgacgaaata ggctttctcg ccttcgacaa ccgcaatgcc
     1921 gacctcctgt tccaggtcgt cagccgccgt tacgagaaga aaagcaccgt cctcaccacc
     1981 aatctcgcct tccgcgactg gccgacgatc ttcccgaacg ccacgtgcgc gaccgccctc
     2041 atcgaccgcg tcgtccatca cgccgacgtg atcgccatcg agggagagag ctaccgcgct
     2101 cgcgaggccg agcttcacgc caaggaccgg cgctctcgca ggagggagaa gcccgagccc
     2161 gaacccgccg cttgacctgt cagctgtgct ccggaggaca gcccgaccgc gactgcgtcg
     2221 cgctcgggct gttcagattt cgacggaaat cggtgctcgc caacacgggg caaccgccga
     2281 cgccgagaac cgaagcgcca cccccgccgc cgccgccgcg cgagcc
//
LOCUS       DASTKS010001267         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_873430, whole genome shotgun sequence.
ACCESSION   DASTKS010001267 DASTKS010000000
VERSION     DASTKS010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873430"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1733
                     /locus_tag="VFQ35_27085"
     CDS             141..1733
                     /locus_tag="VFQ35_27085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9934409.1"
                     /translation="MPTRHVLAWDLGTSGAKVGIVAPSGVVLGTEFEPVGLSLSNGGA
                     EQSPDEWWKALVLATRRLLAREMVPTQSIAAVGVTAQWSGTVMVDAQGRALGNAVIWM
                     DTRGAPAVKRLTGGFPEVQGYALHRLWRWMRLTGGAPTHAGKDPIGHILWLREARPEL
                     YARTHKFLEPKDYLISRLTGRFTATFDSIAMHWVTDNRDPRHIDYDAGLLDLVGLEQK
                     QLPELCGAPDIVGTLLPGCAEELGLDASVVVVGGAADVHSGAIGAGTTRDFATHLYVG
                     TSSWISCHLSAKKTDVLHNMAALPAAIPGRYLLLNAQETAGACLTHVADNLLFADDEL
                     GTPAPPNVFELFDRVAARAPAGSGRLLFLPWLYGERTPVEDARLRGGFVNYSLEHRRS
                     HVLRSVLEGVAYNARWLFGHVERFVAKSIPELRFIGGGANSNLWCQIFADVLGREIAR
                     VDAPRSSNLRGAGLIAFVGLGEFDFDEVAERVPVERRFEPKPELRALYDELFAEFLEL
                     HDKNRGILRRLNRDLTPALPTP"
     gene            1730..>2889
                     /locus_tag="VFQ35_27090"
     CDS             1730..>2889
                     /locus_tag="VFQ35_27090"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9934410.1"
                     /translation="MTRRTPSLLNKAARGISSRLFDFAYDNARRIGPLRRRIDQRLDT
                     LLDPATSGLRPYRDAASFHSLPAQGVPRAEVLALIDELATKERAHWAQGNASGAVYRS
                     DPEHLDFQNEVYARCAQSNPLHTDIWPSASKFEAEIVSMCARLLGAPAEEPSDEAERI
                     SGTVTSGGTESIILAMKSYRDHAWSSHRRARRQILAPESAHAAFDKAAQLLGLELVRI
                     PVGTDYRADLGSMRRAIGPHTLVTVASAPCFPHGVVDPVREIAKLASEHDVGLHVDAC
                     LGGFVLPFARELGYPVPEFDFRVPGVSSMSIDTHKYGYAAKGTSVVLYRGADLYRQQY
                     FVSTEWSGGLYFSPTLAGSRPGGLSAACWASLVSVGHAGYVDATRRLLETAA"
BASE COUNT          466 a          974 c          963 g          486 t
ORIGIN      
        1 caagaaacgc gagcgagaag cgcgcccgag gagtgaggac gaacggcgcg ggacagctcg
       61 agtacgcatt cctagcgaat tcaggcgggg gcctcgacga ggtggtgcgc gccgggtgca
      121 accggtaaag ttttgggagc atgccgactc gccacgtgct cgcctgggat ctcggtacgt
      181 ccggcgcaaa agtaggcatc gttgctccct ctggcgtcgt gctcgggacc gaattcgagc
      241 cggtcgggct ttcgctttcc aatggcggcg ccgaacagag cccagacgag tggtggaaag
      301 cgctcgtgct cgcgacccgg cgactgctcg cccgcgagat ggtgccaacg caatcgattg
      361 ccgcggtcgg cgtgacagcg caatggtccg ggacggtgat ggtggacgcg cagggtcgcg
      421 cgctgggcaa cgccgtgatc tggatggaca cgcgcggagc tccggccgtg aagcgcctga
      481 ccggaggctt ccccgaagtc caggggtacg cgctgcacag gctgtggcga tggatgcgcc
      541 tcaccggcgg agccccgacc cacgctggca aggatcccat cgggcacatc ctctggctac
      601 gcgaggcgag gccggagctc tacgcgcgaa cccacaagtt cctggagccg aaggactatc
      661 tcatctcgcg cctgacgggt cggttcaccg cgacgttcga ttcgatcgcg atgcactggg
      721 tcaccgataa ccgcgaccca cgacacatcg actacgacgc gggcctcctc gatctggtcg
      781 gactagagca gaagcagcta ccggagctct gcggggcacc cgacatcgtc ggcacgctgc
      841 tcccgggctg tgcggaagag ctcggtctcg acgcgagcgt cgtcgtggtg gggggcgcgg
      901 cagacgttca ctccggagcc atcggtgcgg ggacgacgcg cgatttcgcg acgcaccttt
      961 acgtcggcac ttcgtcgtgg atttcgtgcc acttgtcggc aaaaaagacc gatgtgctcc
     1021 acaacatggc ggcgctgcca gccgcgatcc cggggcgcta cctgctcttg aacgcgcagg
     1081 agacggcagg cgcctgcctg actcacgtcg cggataatct gctctttgca gacgacgaac
     1141 tcggcacgcc ggcgccgccg aacgtattcg agctcttcga tcgcgtggct gctcgtgcgc
     1201 cggcgggcag cggacgcctt ctattcttac cgtggctata cggggagcgc actccagtgg
     1261 aggacgcgcg cttgcgcggc gggttcgtca actactcgct cgagcaccgg cgctcgcacg
     1321 tgctgcgctc cgtgctcgag ggcgtcgcct acaatgctcg ctggttgttc ggtcacgtcg
     1381 agcgcttcgt cgcgaaatcc atccccgagc ttcgcttcat cggcggcggg gcaaactcga
     1441 atctatggtg tcagatcttc gccgacgtcc tcgggcgcga gatcgcgcgg gtcgacgcgc
     1501 cgcgttcctc gaacctgcgc ggagcgggcc tcatcgcgtt cgtcggcctc ggagagttcg
     1561 acttcgacga agtcgcggag cgcgtcccgg tcgagcgccg cttcgaacca aagcccgagc
     1621 tgcgcgcgct ctacgacgag ctctttgccg aattcctgga gctccacgac aaaaatcgtg
     1681 gcattctccg ccgcctcaat cgcgacctca ctcccgcgct gccgacaccg tgactcgccg
     1741 taccccttcc ctcctcaaca aggccgcgcg cggcatcagc tcgcggctct tcgacttcgc
     1801 ctacgacaac gctcgccgca tcggcccgct gcggcgccgc atcgaccagc gcctcgacac
     1861 gctcctcgac ccggcgacgt cggggctacg accgtaccga gacgcggcga gcttccacag
     1921 cttgccggca cagggcgtcc cacgcgccga agtcctcgcc ctcatcgacg agctcgcgac
     1981 gaaggagcgc gcgcactggg cgcagggcaa cgcatccggc gcggtatatc gttcggaccc
     2041 cgagcatctc gattttcaaa acgaggtgta cgcgcgctgc gcgcagagta acccgctcca
     2101 caccgacatc tggccgagcg ccagcaagtt cgaagccgag attgtctcga tgtgcgcgcg
     2161 cctgttgggg gcaccggccg aagaaccgtc ggacgaggcc gagcgcattt cgggcacggt
     2221 cacgtcgggc gggaccgaaa gcatcatcct tgcgatgaag tcgtatcgtg atcacgcctg
     2281 gagctcccat cggcgcgcgc gccgtcaaat cttggctccc gagagcgcgc acgcggcttt
     2341 cgacaaggcc gctcagctct tgggcttgga gctcgtgcgg atcccggtcg gcaccgacta
     2401 tcgcgccgat ctcggatcca tgcggcgcgc catcgggccg cacacgctgg tgacggtcgc
     2461 ttcggcgccg tgcttcccgc acggggtcgt cgatccggtg cgggaaatcg cgaagctcgc
     2521 gagtgagcac gacgtcgggc tgcacgtcga tgcgtgtctc ggcggcttcg tgctgccgtt
     2581 cgcgcgagag ctcggctacc cggtgccgga gttcgatttc cgcgtgccgg gcgtcagctc
     2641 gatgtcgatc gacacgcaca agtatggcta cgccgcgaag ggcacgtcgg tggtcttgta
     2701 tcgcggcgcc gacctctatc ggcagcagta cttcgtgagc acggagtggt ccggcgggct
     2761 ctacttttca cccacgctcg ccgggagccg cccgggcggg ctcagcgcgg cgtgctgggc
     2821 gtccctcgtc agcgtcgggc acgctggcta cgtggacgca acgcgccggc tgttggaaac
     2881 ggcggcgca
//
LOCUS       DASTKS010001268         6474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1545554, whole genome shotgun sequence.
ACCESSION   DASTKS010001268 DASTKS010000000
VERSION     DASTKS010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545554"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1706
                     /locus_tag="VFQ35_27095"
     CDS             165..1706
                     /locus_tag="VFQ35_27095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9934411.1"
                     /translation="MLGTFREHSPKEVIVRFLKSVFSPVLVLAALPIWSQSANAQTTA
                     PATAPAAPSAPVSEAASVFEARLSKELGVGGGLTADSLAASAVSTNYTVQSKHSQAES
                     AAAQTEKARWGYLPRVTLQARYTRLSSIGVPVLGSLVAAPGQPEGVLPPGATLVNVPL
                     SFPQVLDQYSLTAGLLVPVSDYFFRVAPATSASAANERAARAETDSTARAVATDAKGL
                     YYAWVGARLTAIVAELSLEQARAHGEDVKHALEAGTASPADKLRVDSRIADAERMVES
                     ARHASADLEEQIRIARHDPDNATYAIGEDVRGEVPAGALSDDLRVLVRQALEHRPEFQ
                     ALAARSEAASRAVSLERAAYLPRLDLFADGTYANPNQRYFPQQKEWKGTWDAGAQLTW
                     VVTDVPAAAASTRAAEANLASLRSEVRALEDRVRREVSQALQARLDARSAVRTSKTGL
                     DAAEESYRVRRALFQNGRATSTELLDAELELTRARLAALNAGLELRMSTARLSYAVGE
                     GKPGS"
     gene            1927..3669
                     /locus_tag="VFQ35_27100"
     CDS             1927..3669
                     /locus_tag="VFQ35_27100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934412.1"
                     /translation="MVSAPDPVQVGASTLESPVVVHFGGDRASGFRGAARSERNSLTL
                     ALPFGETPLDSQAGREFLQRRLALFSAVAATLGGFALFAGQLVESALPHYHFPSSFLG
                     NGGWFQLLAIGVSLAVWAAARRGNMSLRALLVLDVLGTLLMLSAYAKMASMSGHRVPQ
                     RVDLMMMLITLCVVMLRAVVIPSPWPHTLVIGAIGSIPALELARQNAAAVVIEGSPTP
                     PWAAMGYMGVWIAVALAISVVTSRVIYGLRCQVAQVERLGQYVLEEKIGEGGMGVVYR
                     ARHALLRRPTAIKLVLPERAGPDNLARFEREVQLTASLTHPNTVSVYDYGSTPEGVFY
                     YAMEYLDGVDLEQLVMRFGPQSPARVAHILSQVAASLGEAHSIGLVHRDIKPGNIILC
                     ERGGVADTAKVVDFGLVKSVSSLGREGQGPTLSSANTIVGTPLYLAPEGLTSPDRIDA
                     RADLYALGAVGYYLLTGAPVFRGQSLLEVAAQHLHQAPVPPSERVEQPIPRALEALIM
                     RCLAKNPLERPQSANELISELKSCGVSEWTLSDAGGWWQENGATARARMRSRSDGKGP
                     GTVVIDLARRVAPA"
     gene            3708..4970
                     /locus_tag="VFQ35_27105"
     CDS             3708..4970
                     /locus_tag="VFQ35_27105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9934413.1"
                     /translation="MAASSRRIVLASLVGTAVEFYDFYIYATAASLVFGPLFFPAASA
                     SAQLLSAYASFSIAFIARPLGASVFGHFGDRIGRKSTLVASLLAMGGSTLAIAFLPTY
                     ATAGALAPVLLCLLRFGQGFGLGGEWGGAALLAVENAPPGYRARFGMFPQLGAPVGFI
                     ASNGVFLLLGLTLPRDAFQMWGWRLPFLGSAVLVGVGLWVRLKITETPAFARVLKEGP
                     PPRVPLAEVLKGHPRAVIAGTFAVVACYAIFYLSTAFALGYGTTTLGYARDTFLGVQL
                     VAILFMAAGIVAAGYLADRLDPRRVLIGGCICAIFAGFLLAPLLGSKNLFEVGVFLSL
                     ALWLMGFVYGPLGAWLPSLFPARVRYTGSSLAFNVGGILGGALAPIIAQALALRGGLS
                     LVGLYLSGAALVSLIALLSLPRSADAST"
     gene            complement(4975..6249)
                     /locus_tag="VFQ35_27110"
     CDS             complement(4975..6249)
                     /locus_tag="VFQ35_27110"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934414.1"
                     /translation="MKIVIVTAGSLGDTAPYVGLGARLKAAGHDVAVAAQRTFETTIV
                     EAGLEFREMPGDVRALLASDDGQRMHRARSLMEALPPTLRFAQTLLSEVTSGIVEASR
                     GAELLILHRLVLLQGRVVAHALGIPCLALELFPSGLVPTSEFLPATLRATSLGEFGNR
                     AVYGLLRALAEGRRQDRLLQELEERLALPRTSPAHWYRRMRAERWPVYHAFSSFVVPR
                     PKDWPEHAKIVGYLWPRIAPRFRAPDRLVDFLKSGARPVFVGLGSLVPEQSERLARLV
                     VAALRRAKVRAVLQGGWGDYVHAGSDDVLCIGDVPHTWLFPQVAAVVHAAGAGVTAAS
                     LRASVPSVCIPAMNDQFFWAERLRRLGVSPGFIRFQDLSAPTLALLLRGALADPSYSR
                     AARRASEALRTEDGAGRIVEVVAEIAERARAA"
     gene            complement(6246..>6474)
                     /locus_tag="VFQ35_27115"
     CDS             complement(6246..>6474)
                     /locus_tag="VFQ35_27115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934415.1"
                     /translation="RIFDGWKPQPIRTPIVVVRARECIPEWEGERLNDDFWRGSWDLP
                     HEILEVPGDHFTVVNQNAKTTALALIDWFSR"
BASE COUNT         1011 a         2159 c         2143 g         1161 t
ORIGIN      
        1 cccgcgagaa gggcggtttt ctggtcgctc gggcggccgc tgcccggacg attgtccgcc
       61 cgcggacagc tgtccccacc gccgggggca aggagctggt gccgaagcca tgacggactt
      121 aggcactggg cgcgcgcttt cttccgcccc gactcgccaa aagcgtgctt ggcacgttcc
      181 gtgaacacag cccgaaagag gtcatcgtgc gttttctgaa atcagtgttc agtcccgtgt
      241 tggtgctcgc cgcgcttccc atctggagcc agagcgcgaa cgcccaaacg acggcgcctg
      301 cgactgcgcc cgcagcgccg tccgcgcccg tttccgaagc cgcatccgtc ttcgaagcgc
      361 gcctttcaaa agagctcggc gtcggcggtg gtctcacggc agattctttg gcagcgagcg
      421 cagtcagcac gaactacacg gtgcagtcga agcattccca ggccgaatcg gcggccgcgc
      481 aaaccgaaaa agcgcgttgg ggctacctcc cgcgcgtcac cctccaagcg cgatacaccc
      541 ggctttcttc gattggcgtg ccagtgctcg gcagcctcgt cgccgctccg ggccaacccg
      601 aaggcgtgct gcctccgggc gctacgctcg tgaacgtgcc gctgagtttc ccgcaggtgc
      661 tcgaccagta ttctctgacc gcgggcctgc tcgtgccggt ttcggactat ttcttccgcg
      721 tcgcgccggc gacttcggcg agcgcggcga acgaacgcgc agcgcgagcc gagacggatt
      781 cgacggcgcg cgcggtcgca acggacgcga aggggctcta ctacgcctgg gtcggcgcaa
      841 ggctcacggc catcgtggcg gagctttcgc tcgagcaagc gcgcgcgcat ggcgaagacg
      901 tcaagcacgc gctcgaggcc ggcacggctt cgcccgccga caagctgcgc gtcgattcgc
      961 ggattgccga cgcggagcgc atggtcgaga gcgcgcgtca cgcctcggcg gatctcgagg
     1021 agcaaatccg tatcgcgcgg cacgatcccg acaacgcaac ctatgccatc ggtgaagacg
     1081 tgcgcggcga ggtcccagcc ggcgcattgt ccgacgatct gcgagtcctg gtccgccagg
     1141 cgctcgagca ccgccccgag tttcaagcgc tggcggcacg ctccgaggca gcgtctcgcg
     1201 cggtctcgct cgagcgcgct gcgtacctgc cgcgcctcga tcttttcgcc gacggcacct
     1261 acgcgaaccc gaaccagcgc tacttccctc agcaaaaaga gtggaaggga acctgggacg
     1321 ccggagccca gctcacgtgg gtagtcaccg acgttcccgc cgccgccgcg agcacgcgag
     1381 ccgcggaagc gaatctcgcg tccctgcgaa gcgaggtgcg agcgctcgag gatcgggttc
     1441 gtcgtgaggt ttcgcaagcg ctgcaggcgc ggctcgacgc tcgctcggcc gtgcgcacca
     1501 gcaagactgg cctcgacgct gccgaagagt cgtaccgagt ccgtcgcgcg cttttccaga
     1561 acgggcgagc gacgagcacc gagctactgg acgccgaact cgagctcacc cgggcacgcc
     1621 tggccgccct caacgcgggc ctcgagctgc ggatgtctac tgcccgcctg agctacgcgg
     1681 tgggggaagg gaagcccggg tcctgaccag caaccggcac gcacccacgt aacttatcgg
     1741 aaacattaaa tctttgataa atttgtcgct tctcgataaa gagtgaggaa ggtggcgcgt
     1801 agagaaactt gggcccaggt cggggcgtag gagagcagga atgtcacagg ttcgcgtggg
     1861 tacccgttcg gacgccgttc ccacggtggt tcgagggagg tgcgccgagc ggaccgagcg
     1921 ggtgcggtgg tgagcgcccc cgacccagtg caggtcggcg cttcgacctt ggagagcccg
     1981 gtcgtcgtcc acttcggtgg tgaccgggcg agtggcttcc gtggcgcggc tcgctcggag
     2041 cgaaactcgc tcacgctggc gttgccgttc ggtgagaccc ccctcgatag ccaagcgggg
     2101 cgagagtttc ttcagcgccg gctcgcgctg ttctccgcgg ttgcggctac cctcggcggc
     2161 ttcgctctgt tcgcggggca gctcgtcgag tcagcgctgc cgcactatca ctttccgtcg
     2221 tcattcctcg gcaacggggg gtggtttcag ctgctcgcca tcggcgtatc gctcgcggtg
     2281 tgggccgccg ctcgccgcgg caacatgtcg ctgcgtgcgc tcctcgtgct cgatgtcctt
     2341 ggaacgctct tgatgctgtc ggcgtacgcc aagatggcgt cgatgagcgg ccatcgcgtc
     2401 ccgcagcgcg tggacctcat gatgatgctc atcacgcttt gcgtcgtgat gttgcgcgcg
     2461 gtggtcatcc cgagtccatg gccgcacacg ctcgtcattg gggcgatcgg ctcgatccct
     2521 gcgctggagc tcgctcgcca aaacgcggcc gcggtcgtga tcgaaggtag cccgactccg
     2581 ccctgggcgg cgatggggta catgggggtt tggatcgccg tggcgctcgc gataagcgtc
     2641 gtcacgtcgc gcgtaatcta cggcttgcgc tgccaggttg ctcaggtcga gcggctcggc
     2701 caatacgtgc tcgaagagaa gatcggcgag ggcggcatgg gcgtggtcta ccgcgcacgt
     2761 cacgcgcttt tgcgccggcc aaccgccatc aagctcgtgc tccccgagcg cgctgggcca
     2821 gacaatctcg cgcgcttcga acgcgaggtg cagctcacgg caagcctcac gcatcccaac
     2881 acggtgagcg tgtacgacta tgggagcact ccagagggcg tcttctacta cgcgatggag
     2941 tacctggacg gcgtcgacct cgagcagctc gtgatgcgct tcggcccgca gtcgccggcg
     3001 cgcgtcgcgc acattttgtc gcaggtcgcg gcctcgctcg gcgaagcgca ctcgattggc
     3061 ctcgttcacc gcgacatcaa gcccgggaac atcatcttgt gtgagcgcgg cggcgtcgcg
     3121 gataccgcca aagtcgtcga cttcggcctc gtgaagagcg tctcgtcgct cggtcgcgaa
     3181 ggtcagggcc cgacgttgag cagcgccaac accatcgtcg gcactccgct ttacctcgcg
     3241 ccggaggggc tgacgtcgcc cgatcgcatc gacgcgcgcg cggatctcta cgctttgggc
     3301 gcggtcggtt actacctact gacgggtgct cccgtatttc gcgggcagag cctgctcgaa
     3361 gtcgcggcgc agcacctgca ccaagcgccg gtaccgcctt cggagcgcgt cgaacagccg
     3421 atcccgagag cgctcgaagc gctcatcatg cgctgcttgg ccaaaaatcc gctcgagcgt
     3481 ccacagtcag caaacgagct catcagcgag ctcaagagtt gcggcgtcag cgagtggacc
     3541 ctgagcgacg cgggtggttg gtggcaggag aacggcgcta cggctcgtgc acgcatgcgc
     3601 tcgcgctccg acggcaaagg gccgggcacg gtagtcatcg atctcgcgcg gcgagtcgcc
     3661 ccggcttgac gtagcgccga gaatggccga ttcgatcgag ccacctcatg gcggcttcgt
     3721 ctcgacgcat cgtgctcgcg agcctcgttg gcacggcggt cgaattctac gacttttaca
     3781 tctatgccac ggccgcctcg ctggtcttcg gcccgctctt ctttccggcc gcctccgcct
     3841 cggcgcagct cttgagtgct tacgcgagtt tcagcatcgc gttcatcgca cggccgctcg
     3901 gcgcgagcgt gttcggccat ttcggcgatc gaatcgggcg taagtcgacg ctcgtcgcgt
     3961 cgttgctcgc gatgggtggc tcgaccctcg cgatcgcctt cttgccgact tatgcgaccg
     4021 ccggtgcgct cgcgccggtg ctgctgtgct tgctccgctt cggtcaaggc tttggcctcg
     4081 gtggcgagtg gggtggggca gcgctgctcg cagtcgagaa cgcaccgccg gggtatcgcg
     4141 ctcgctttgg gatgtttcct cagctcggcg cgccggtcgg cttcatcgct tcgaacgggg
     4201 tgttcttgct cctggggctc accctgccgc gcgacgcttt tcaaatgtgg ggctggcgtc
     4261 tgccgtttct cgggagcgcg gtgctcgtcg gcgtcgggct ctgggtgcgc ctcaagatca
     4321 ccgaaacgcc agcctttgcg cgtgtgttga aggaagggcc gcccccgcgc gtgccgctcg
     4381 ccgaggtgct caaggggcat ccccgcgcgg tgatcgctgg cacgttcgcg gtcgtcgcct
     4441 gctacgcgat cttttacctc tccactgcct tcgcgctcgg ctacggcacg acgacgttgg
     4501 gttatgcgcg cgacaccttc ctcggtgtgc agctcgtcgc gatcttgttc atggcggcag
     4561 gcatcgtcgc cgccggctat ctcgccgatc ggctcgaccc gcggcgggtg ttgataggcg
     4621 gctgcatttg cgcaattttt gccgggtttt tgctcgcgcc gctgctcgga agcaaaaatc
     4681 tgttcgaggt cggcgtgttc ctctcgctcg cgctgtggct catggggttc gtgtacgggc
     4741 ccctcggagc ctggcttcct agcctgttcc ctgcgcgcgt tcgctacact ggctcgtcgc
     4801 tcgcattcaa cgtcggcggg atcttgggcg gagcgctggc tcccatcatc gcgcaggctc
     4861 tcgcgttgcg aggcggactc tcgttggttg gcctctacct gagcggtgct gcgctggtga
     4921 gcctcatcgc gctgctgtcg ttaccgaggt cggccgacgc ctcgacgtga tccgtcacgc
     4981 cgctcgggct cgctccgcga tttcggcgac gacttcgacg atgcgtccgg cgccgtcctc
     5041 tgtgcggagt gcttccgagg cgcggcgcgc cgcgcgcgaa tacgacggat ccgcgagggc
     5101 tccgcgaagc aagagggcga gcgtcggcgc agacaaatcc tgaaagcgta tgaaaccggg
     5161 gctcacgccg aggcgcctca ggcgttccgc ccaaaagaac tgatcgttca tcgccggaat
     5221 gcaaacggag ggcaccgagg cgcgcaggct cgcagccgta actccggcgc ccgcggcgtg
     5281 aaccaccgcc gcgacctgcg gaaacagcca cgtatgcggg acatcgccga tgcagagcac
     5341 gtcgtcgcta cccgcatgaa cgtagtctcc ccagcctccc tggagcaccg cacgcacctt
     5401 ggcgcgccgc agcgcagcca cgacgagtcg agcgagccgt tccgactgct cgggcaccaa
     5461 gctgccgaga ccgacgaaca cgggccgcgc ccccgacttc aggaagtcga cgagcctgtc
     5521 cggagcgcgg aaccgaggcg cgatgcgcgg ccagaggtaa ccgacgatct ttgcgtgctc
     5581 gggccagtcc tttggccgcg ggaccacgaa cgaactgaat gcatgataga cgggccagcg
     5641 ctccgcgcgc atgcgccgat accaatgcgc agggctcgtg cggggcagcg cgaggcgctc
     5701 ctcgagctcc tgcagcagcc tatcttggcg ccggccttcg gcgagcgcgc gaagcagccc
     5761 gtacaccgca cggtttccga actccccgag tgaagtggcg cgaagagtgg ccggcaagaa
     5821 ttcgctcgtc ggtacgagcc cgctcgggaa caactccagc gcaaggcagg gaatgcccaa
     5881 agcgtgggcg acgacccgac cttgcaacaa aacgagtcgg tgcaggatca agagctcggc
     5941 gccgcggctc gcttcgacga tgccgctcgt tacttccgag agaagcgtct gcgcaaaccg
     6001 aagtgttggc ggcaaagcct ccatcaacga gcgggcgcgg tgcatgcgct gcccgtcgtc
     6061 ggaagcgagc aatgctcgaa cgtcgcccgg catttctctg aactcgagcc ccgcctcgac
     6121 gatcgtcgtc tcgaatgtgc gttgagccgc gaccgcgacg tcgtgacccg ctgctttgag
     6181 ccgggcgccg aggccgacgt aaggcgccgt gtctccgagc gagccggcgg tgacgatgac
     6241 tatcttcacc gcgagaacca gtcgatgagg gcgagggcag tcgtcttcgc gttttggttc
     6301 actaccgtga agtgatctcc aggcacttcc aagatttcgt gcgggagatc ccaagagcca
     6361 cgccagaagt cgtcgttcag gcgttcgccc tcccactccg gaatgcattc gcgagctcgc
     6421 acgacgacga ttggcgttcg aatcggctgc ggtttccagc catcgaagat gcgg
//
LOCUS       DASTKS010001269         9828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_426445, whole genome shotgun sequence.
ACCESSION   DASTKS010001269 DASTKS010000000
VERSION     DASTKS010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426445"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..654
                     /locus_tag="VFQ35_27120"
     CDS             166..654
                     /locus_tag="VFQ35_27120"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9934416.1"
                     /translation="MSEIVQVSKAATRNAASEQLLTFMVDGQEYGVDILRVQEIRGWT
                     APMPIPNAPPYIKGVINIRGDVVPIADLRERLGLPSLQYGQTTVVVVLRVQHANRERV
                     MGVIVDAMSDVTNVPSDAVKAPPTFAEVSEAPLARGIATFEEKMITILDVDRIFGEPP
                     VR"
     gene            694..2304
                     /locus_tag="VFQ35_27125"
     CDS             694..2304
                     /locus_tag="VFQ35_27125"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9934417.1"
                     /translation="MPADKAETESNDLVRLSSALDGTTSAIMMVDRDLVITYANRSTR
                     ELVTKHESTFRAAFPSFKAANLVGSCIDMFHVKPERQRQMLANPNNLPHAADIKVGPL
                     TFRITVTAIIDPGGNYLGNTLEWQDVTEQRDGERQIQLLIDAATAGRLDVRIDSEKYS
                     GFMKTMSGGVNRLMDTVVGPIRETMRVVECMASGDLRSEMDGDFSGEFATLRDRLNET
                     LRTLRDTVAQLQRSASEIDSASSDISEGNANLNKRTQEQSSALEETASSLEEMTATVK
                     QNAANATQANQLAASARDSAEKGGQVVGAAVSAMTAITESSKKVADIIGVIEQIAFQT
                     NMLALNAAVEAARAGDQGRGFAVVAAEVRNLAQRSAAAAKEIKSLIQDSQDKVDQGSK
                     LVTRSGETLQEIVSSVKRVSDIVGEINAASDEQASGIDQINAAVNQMDKSTQQNAAMV
                     EEAAAAAESMNEQARALTDLVAFFKTGNVVAASEPAPPPARKAEARPQAKPKKAQPAA
                     RPERSAPAAKSANGKRPAQSEDGEWAEF"
     gene            2325..3182
                     /locus_tag="VFQ35_27130"
     CDS             2325..3182
                     /locus_tag="VFQ35_27130"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9934418.1"
                     /translation="MSASLSPNSTEFELSDEAFARIQVLLKQRSGIDLGPGKRMLVYG
                     RLARRLRALRLSSFSEYLDLVDDPASEEARHFLNACTTNVTELFREPHHFEMLTNRAI
                     PEALGRSRERKIRVWSAGCSTGEEPYSIAISLHEAGLVNGYDVKVLATDIDSDVLAHA
                     ERGVYAADKVGKLKPAQRRYFQRGLAEKSNLARVRPDLKSIIRFRQLNLMNEWPMSGR
                     FDVIFCRNVVIYFDTPTRERLVRRFCSLLHPGGYLFMGHSESLTAGSATELVPCGKTA
                     YRKPAEDDE"
     gene            3179..3808
                     /gene="cheD"
                     /locus_tag="VFQ35_27135"
     CDS             3179..3808
                     /gene="cheD"
                     /locus_tag="VFQ35_27135"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HET9934419.1"
                     /translation="MKVRVATAGAPPQLPGFVGVHRFWDSADGHWVAQVLPGEFYVTR
                     SAEVIATTLGSCVSTCIRDPVIGIGGLNHFMLPEDPRNDAGGGAMRYGFFAVERLINV
                     LIKHGARRENLEIKIFGGGKVIPGMSDVGRANIEFVRGYFETELLDIVAEDVGGNYAR
                     RVRYYPETGRAMVKRLPTGEANEVVRGERALKERLSMQPAAGEVELFGD"
     gene            3830..4864
                     /locus_tag="VFQ35_27140"
     CDS             3830..4864
                     /locus_tag="VFQ35_27140"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9934420.1"
                     /translation="MGTKVLIVDDSALVRRLLSGLIARDPRFEVVGTAVDPYDAREKI
                     KALNPDVLTLDVEMPRMDGLTFLRNLMRLHPMPVVMVSSLTERGADVTIEALETGAID
                     FVTKPQAGVADGLAKLSEELCEKLATAATARVTTRERPTRDGNAPLKPTLLKTTDRLI
                     AIGASTGGTEAIAQMLEEFPADAPAVVITQHIPELFSARFAARLDSQCNLSVAEAKDG
                     DPILLGHAYVAPGNQHLRVVRSGARYHCQLSQEAPVNRHRPSVDVLFESVAKNVGANA
                     VGVILTGMGDDGAEGLGMMKAAGSRTIAQDKTSSVVWGMPGEATKRGHAGEVLPLERI
                     AARVLELSRL"
     gene            4976..5536
                     /locus_tag="VFQ35_27145"
     CDS             4976..5536
                     /locus_tag="VFQ35_27145"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9934421.1"
                     /translation="MTTTTSVSPNSTETPTTSLMVLGEALRVSCVELMQNYGIEARPR
                     GPEAASEAPVAVLVAGVDFKGRELRGTVALWAAQSVLRETTRKAPGLGENAPLQDWTC
                     ELTNQLAGRMKNKLRPYGVSLHVNVPRLLHGSDVTELETGLQHRFSCDFGSLSAYLDV
                     LIAPGFVLTEGEPTEPLKQEGDFTVF"
     gene            5554..5937
                     /locus_tag="VFQ35_27150"
     CDS             5554..5937
                     /locus_tag="VFQ35_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934422.1"
                     /translation="MAAKILIIDDSASVRRQVSSILSSEGFDIIEAADGIEGSAQIKA
                     NNDLALVLCDINMPRLNGLDMLESLTEEIAKHAIPVVMLTTEGEPESMTRARKAGARG
                     WIIKPFKDQLLVGAVKKLTGVEAVR"
     gene            6017..6991
                     /locus_tag="VFQ35_27155"
     CDS             6017..6991
                     /locus_tag="VFQ35_27155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934423.1"
                     /translation="MFARNLAALVVALSVSTGVLVLTSSASAEPCAPADRDCAKRSYE
                     AGTAAFDRGEFAQALAAFESARAANSHPFVSFNIALCLARLGRLLESRRELDKLLEDA
                     TLGGDLEKRVKTERAQVNAQLSRIILDAPSSGYAVEIDGAPTVGEKELELDPGEHHLR
                     ITKGEQLVYDQVVKLDSAERLRLRVTDQERAIDVVVVPEQRSTKASTAPSPAPAPRRP
                     LSPVVFYAAASASVLLGGLTVWSGLDVQSAYDDYKRDLPHLTQSQADDRVSSGHAREL
                     RTNVLLGATALSVAATATIGLLWVDFGGRSDAVALGFDGQSVRVRAAF"
     gene            complement(7002..7862)
                     /locus_tag="VFQ35_27160"
     CDS             complement(7002..7862)
                     /locus_tag="VFQ35_27160"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9934424.1"
                     /translation="MQRTITSDRPPAHRLKAPIVVVTPVGEFEHERGGLVLGRAEDAN
                     VVLDDPLISRAHARLVVLADETVVVEDLHSTNGVFVNGVRLARPSYRLSEGDRLLIGT
                     TEVSVFSSRASATIPIEHHTGEPMKRGVAVPGAAAPRPDASMVPVTNRSNAVELVGNL
                     ARRLMENGRGAEAVRVLTEHLNNVLLGASAGLSVPDIVLQRASEHALELFRWTARAAW
                     IDYVFQLHLSCQRVPVMSVVALIERELEKHPAVDRELIDYFASSLRARAATLTADEQL
                     VLARIALWTP"
     gene            8218..>9828
                     /locus_tag="VFQ35_27165"
     CDS             8218..>9828
                     /locus_tag="VFQ35_27165"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9934425.1"
                     /translation="MVEVSVVLVNGKSHTGLLARFNPMAPDVALSLAQDAKGSSRFAA
                     EQVAFVAFHRAPGDPPPPPTRRGTLKIHVSGGKTFVVDPVDPSPPGAIGAYMRPAEAQ
                     SPYREIFFYNHGVNLREVNEPIGVLLVKEGRLGAEAVQAGLLAQKDEKRTPIGQILIE
                     NKRINQAALDQATALQQRKGTRIGEVLIEAGLADQADIDFALSEQRRRGGKRIGQILV
                     EMNLISEVDLAVALARKFQLPFVNLDECSINVDAVAELPRDFVEKNRVLPVESDPRSI
                     TVALSDPLAVDTLDLVRIHSKKQLSEVVVTRSQLDKYIPTYLDQLEAKNRANEMDEIL
                     KGLASGDAQAVTPEEATEATVKDTDNAIIKLANQIIIDAYRRGASDIHIEPNGRERIT
                     NVRFRVDGDCITYQDIPSAYRLPLVARFKIMAQLDISERRKPQDGKIRFRVGERQIEL
                     RVATIPTVNNNEDIVLRILAGSKPLPLEQMGMSARNLAEFRSAIKKPYGLVLCVGPTG
                     SGKTTTLHSALGDINTVDTKIWTAEDPVE"
BASE COUNT         1937 a         3239 c         3143 g         1509 t
ORIGIN      
        1 aactacggcc gcgtggatgg cctcgcagga gccaccattc tcggtgacgg cgcagtgtcg
       61 ttgattctcg acatggccgg catcggtcgc ttggtgcgtg gcaccagcac gcgcgatggc
      121 gcccgagcag cgtaaggcaa acagcgaaag tcgagcagac gaaaaatgtc ggagattgtc
      181 caagtatcca aagccgcaac ccgtaacgcc gcttccgagc agcttctgac cttcatggtg
      241 gacgggcagg agtacggcgt ggacatcctg cgagtgcagg agatccgtgg ctggacggcg
      301 ccgatgccga tcccgaacgc gccgccctac atcaagggcg tgatcaatat tcggggcgac
      361 gtggttccga tcgcggacct gcgcgagcgg ctcggtttgc cttcgctgca gtacggacag
      421 acgaccgtcg tcgtggtgct gcgcgttcag cacgcgaatc gcgagcgggt catgggcgtc
      481 atcgtcgacg cgatgtccga cgtcaccaac gttccgagcg atgccgtgaa agcgcccccg
      541 acgttcgcgg aagtgagcga agcgccgctc gcccgaggca tcgcgacgtt cgaggaaaag
      601 atgatcacca tattggacgt ggaccgcatt ttcggtgagc caccggtgcg ttgagggtcc
      661 tcggaatacc aataccagag gagaatcaag cgaatgcccg cagacaaagc cgaaacggaa
      721 tccaacgacc tggtgcgact gagcagcgct ctcgacggaa ccacgagcgc catcatgatg
      781 gtcgaccgcg acctcgtcat cacctacgcg aaccgctcga cgcgagagct cgtgaccaag
      841 cacgagagca ccttcagggc cgcgttcccg agcttcaagg cggcgaacct cgtggggagc
      901 tgcatcgaca tgttccatgt caagcccgag cggcagcgcc agatgctcgc caacccgaac
      961 aacttgccgc acgcggccga catcaaggtg ggaccgctga cgttcaggat cacggttacc
     1021 gcgatcatcg accccggcgg caactatctc ggcaacacgc tcgagtggca agacgtcacc
     1081 gagcagcgcg acggcgagcg ccagattcag ctgttgatcg acgcggccac ggccgggcgc
     1141 ctcgacgttc gcatcgattc cgagaagtac agcggcttca tgaagaccat gagcggcggc
     1201 gtcaaccgac tgatggacac ggtcgtgggc ccgatccgcg aaacgatgcg cgtagtcgag
     1261 tgtatggcct cgggtgacct caggtccgag atggacggcg acttctcggg agagttcgcg
     1321 accctccgcg accgcctcaa cgagacgctc aggacccttc gcgataccgt cgctcagctg
     1381 caacgttcgg cgtcggaaat cgattccgcg tcgagcgaca tctccgaggg caacgccaac
     1441 ctgaacaagc gcacgcagga gcagtcgtcg gcgctcgaag agaccgcctc gagcctcgag
     1501 gagatgaccg ccaccgtgaa gcagaacgcc gccaacgcga cgcaagcgaa ccagctcgcc
     1561 gcgagtgcgc gtgactccgc cgaaaagggc gggcaggtcg tgggcgccgc cgtctcggcg
     1621 atgaccgcca tcaccgagag ctcgaagaag gtggccgaca tcatcggcgt catcgaacaa
     1681 attgctttcc agaccaacat gctcgcgctg aacgccgccg tcgaagcggc gcgcgcgggt
     1741 gaccaaggtc gcggcttcgc ggtggtcgcc gcagaggtgc gcaacctggc gcagcgcagc
     1801 gccgcggcgg cgaaagagat caagagcctc atccaggaca gccaagacaa ggtcgatcaa
     1861 ggctcgaagc tcgtcacccg ctccggcgag acgctacaag agatcgtaag ctcggtgaag
     1921 cgcgtctccg acatcgtcgg agagatcaac gccgcgagcg acgagcaagc gagcggcatc
     1981 gaccagatca acgccgccgt caaccagatg gacaagtcga cgcagcagaa cgccgcgatg
     2041 gtcgaagagg cggcggccgc ggccgaatcg atgaacgagc aagcgcgcgc cctgaccgac
     2101 ctcgtcgcgt tcttcaagac gggcaacgtc gtggcggcga gcgagcccgc accgccgccg
     2161 gcacgtaaag ccgaagcgcg accgcaggcc aagccgaaga aagcccagcc cgccgcacga
     2221 ccggagcgct ctgccccggc cgcgaaatcc gcgaacggca aacgtcccgc ccaatccgaa
     2281 gacggcgagt gggccgagtt ctgagcgtcc ctgcgagcgc aacgatgtca gcttccctga
     2341 gcccgaacag caccgagttc gagttgtccg acgaagcctt cgccaggatc caggtcctgc
     2401 tgaagcaaag gtccgggatc gacctcgggc cagggaagcg catgttggtg tacggtcgat
     2461 tggcgcggcg gctccgcgcg ctgcgcctct cgagcttcag cgagtacctc gacctcgtgg
     2521 acgaccccgc gagtgaggag gcgcgtcact tcttgaacgc ctgcacgacc aacgtgacgg
     2581 agctgttccg tgagccccac cacttcgaaa tgctcacgaa tcgcgcgatc cccgaggcgc
     2641 tcgggcgctc ccgcgagcga aagatccgcg tttggtccgc tggttgctcc accggggaag
     2701 agccctactc catcgcgatt tcgttgcacg aagccgggct cgtcaacggc tacgacgtga
     2761 aggtgctggc gaccgacatc gactcggacg tgctcgcgca cgccgaacgc ggcgtctacg
     2821 ccgcggacaa ggtcggcaag ctcaaacccg cgcagcgtcg ctactttcaa cgcggactcg
     2881 ccgaaaagtc gaaccttgcg agggttcggc ccgacctcaa gtcgatcatc cgatttcgcc
     2941 agttgaacct gatgaacgag tggcccatga gcggtcgctt cgacgtcatc ttctgccgca
     3001 acgtggtcat atacttcgac acgccgacgc gcgagcgtct ggtgcggcgc ttttgttcgt
     3061 tgctccatcc gggcggctac ctattcatgg gtcactccga gtcgctcacg gctggtagcg
     3121 cgaccgagct cgtaccgtgt ggcaaaaccg cttaccgcaa gccggcggag gacgacgagt
     3181 gaaggtccga gtagcgaccg ccggcgcgcc gccgcagctc cctggcttcg tcggtgtgca
     3241 ccgcttctgg gactcggccg acggtcattg ggtagcgcag gtgctccccg gcgaattcta
     3301 cgtgacgcgc tccgcggagg tgatcgcgac gactttgggc tcgtgcgtgt cgacgtgcat
     3361 ccgtgacccc gtcatcggca tcggcggcct caatcacttc atgctcccgg aggacccgcg
     3421 caacgacgcc ggcggcggcg ccatgcgcta cggtttcttc gcggtcgagc gcttgatcaa
     3481 cgtgctcatc aagcacgggg cgcggcgaga aaatctcgaa atcaagatct tcggtggggg
     3541 aaaggtgatc cctggaatga gcgatgtcgg ccgtgcgaac atcgaatttg tacggggcta
     3601 cttcgagacc gagcttctgg atatcgtcgc cgaagacgtt ggcggaaact acgcgcgccg
     3661 cgtacgctac tatccggaga caggccgcgc gatggtgaag cgcctgccta ccggcgaagc
     3721 gaacgaagtc gtacgcggcg agagggcgct caaagaacgc ctgtcgatgc agccggcggc
     3781 cggagaagtc gagctgttcg gcgattgagc aacacggaaa tcgggagaga tgggaaccaa
     3841 agttttaatc gtggacgact cggcgctggt gcgccgcttg cttagtggtc tcatcgcgcg
     3901 cgaccctcgc ttcgaagtag tcggcaccgc ggtcgacccc tacgacgcgc gcgagaagat
     3961 caaggccctg aaccccgacg tgctgacgct cgacgtggag atgccgcgca tggacgggct
     4021 caccttcttg cgcaatctga tgcgcctgca tccgatgccg gtggtgatgg tctcgtcact
     4081 taccgagcgt ggcgcggacg tcaccatcga ggcactggag acgggagcca tcgacttcgt
     4141 cacgaagccg caggccggcg ttgcggacgg cctcgcgaaa ctttcggagg agctgtgcga
     4201 gaagctcgcg acggcagcca cggcgcgcgt cacgacgcgt gaacggccga ctcgcgacgg
     4261 caacgccccc ctaaagccga ctctactgaa gacgacggat cggctgatcg cgattggcgc
     4321 ctcgaccggc ggcaccgagg cgatcgcgca gatgctcgaa gagttccccg ccgacgctcc
     4381 ggcggtggtc atcacccagc acatcccaga gctcttcagc gcgcgcttcg ccgcgcggct
     4441 cgactcgcag tgcaacctct ccgtcgccga agccaaggac ggcgacccga tcctgctcgg
     4501 acacgcctac gttgcgccgg gtaatcagca cctccgcgtc gtgcgcagcg gcgcccgtta
     4561 ccattgccag ctcagtcagg aagcgcccgt caaccgccac cgcccgtccg tcgacgtgct
     4621 gttcgagtcg gtggcgaaga acgtgggcgc gaacgcggtc ggagtcatcc tcaccggcat
     4681 gggcgacgac ggcgcagagg gtctcggtat gatgaaggcc gcgggcagcc gcaccatcgc
     4741 gcaggacaag accagcagcg tggtttgggg catgcccggc gaggccacca agcgtgggca
     4801 cgcgggggaa gttctcccac tcgagcgcat agccgcgcgc gtgctcgagc tcagccgcct
     4861 gtgaacggta gcgcgcacat tttcggcgtt aaccgctcgt ggcgtgagcc gttccttggc
     4921 aacgagcctc ctcacgcgaa gtgggagagg cagcgccgag gaaacagacg acgcgatgac
     4981 cacgaccacc agcgttagcc cgaacagtac ggaaaccccg accacgagtc tcatggtact
     5041 cggggaagcg ttgcgcgtgt cctgcgtcga gctgatgcag aactacggga tcgaagcgcg
     5101 gcctcgaggt ccggaagccg cgtcggaggc gccggtggcc gtgctcgtgg cgggagtaga
     5161 tttcaagggc cgagagttac gcggaacggt cgcgttgtgg gcagcgcaga gcgttctacg
     5221 cgagacgacg cgcaaagcgc ccggcctcgg cgagaacgct ccccttcaag attggacctg
     5281 cgaactgacc aaccagctcg ccgggcgaat gaagaacaag ctgaggcctt acggggtatc
     5341 gctccacgtc aacgtgccga ggctccttca cggcagcgac gtcaccgagc tcgaaaccgg
     5401 gctccagcac cggttctcgt gcgacttcgg ttcgctttct gcgtacctag acgtgctcat
     5461 cgcgccgggc ttcgtgctga ccgaaggcga gccaacggag ccattgaagc aagaaggcga
     5521 cttcacggtc ttctgagagg caaggagaac agcatggccg cgaagatttt gatcatcgac
     5581 gactctgcct ccgtgcgacg gcaggtcagc agcatcctct cgtccgaagg gttcgacatc
     5641 atcgaagccg ccgacgggat cgaaggctcg gcgcaaatca aggcgaacaa cgaccttgcg
     5701 ctcgtgctct gcgacatcaa catgccacgc ctgaacggcc tcgacatgct ggagtcgctc
     5761 acggaagaga tcgcgaagca cgcgatcccg gtcgtcatgc tcacgaccga aggcgagccg
     5821 gagtcgatga cccgcgctcg taaggccggc gcccgtggct ggatcatcaa gccgttcaag
     5881 gatcagctgc tggtaggcgc ggtgaagaag ctcaccggcg tggaagcagt tcgctagtcc
     5941 tccgcgcgcg cgacaaaagg gccggccgcg cgcgccggct tggaaaccgg ccgagcccga
     6001 ggtttaatcg ccaagcgtgt tcgctagaaa cctcgccgcg ctggttgtgg cgctctcggt
     6061 ctcgacggga gtgctcgtgc tcacgtcgag cgccagcgca gagccctgcg cccccgcgga
     6121 cagagactgc gccaagcgct cctacgaggc cggcactgcc gcgttcgatc gaggagagtt
     6181 cgcgcaagcg ctcgccgcgt tcgagagcgc gcgcgccgcc aattctcacc cgttcgtcag
     6241 cttcaacatc gcgctctgcc tcgcgcggct cggccggctc ctcgagtcgc ggcgcgagct
     6301 cgacaagcta ctcgaggacg cgacgctcgg aggcgatttg gagaagcggg tcaaaaccga
     6361 gcgcgcgcaa gtcaacgcgc agctctcgcg catcatcctc gacgcgccct cgtcgggcta
     6421 cgcagtcgag atcgatgggg cgcccaccgt gggcgaaaaa gagctcgagc tcgacccggg
     6481 cgagcaccac ctgcgcatca ccaaaggcga gcaactcgtc tacgaccaag tcgtcaagct
     6541 cgactccgcc gaacgtcttc gccttcgcgt caccgatcaa gagcgggcca tagacgtcgt
     6601 cgtggttccg gagcagcgct cgacgaaagc ctcaacggcg ccgtcccccg cgcccgcccc
     6661 ccgccgcccg ctttcgcccg tggtcttcta cgccgcggcc tcggccagcg tattgctcgg
     6721 cgggctcacg gtgtggagcg gactcgacgt gcagagcgcc tacgacgact acaagcgtga
     6781 tctgccccac ctcacccaga gccaagcaga tgaccgggtc agtagcggtc acgcgcgaga
     6841 gctccgcacg aacgtgctcc tcggagcgac cgcgctctcg gtcgccgcga cggctacgat
     6901 cggccttttg tgggtggatt tcggggggcg cagcgacgcc gtggcgctcg gcttcgatgg
     6961 tcagagcgtg cgtgtcaggg ccgcgttcta agagcttcgg ctcaaggagt ccagagcgcg
     7021 atgcgcgcga gcacgagctg ctcgtccgcc gtcaacgtgg ccgcgcgcgc gcgcagactg
     7081 ctcgcgaagt agtcgatgag ctcgcggtcg accgcggggt gcttctcgag ctcgcgctcg
     7141 atgagggcga ccacgctcat caccggaact cgttggcaag agaggtgcag ctggaacacg
     7201 tagtcgatcc aggcggcccg cgccgtccag cgaaacaact cgagagcgtg ctcgctcgcg
     7261 cgctgcaaaa cgatgtcggg cacggagagc ccagcggagg cgccgagcag cacgttgttc
     7321 aagtgctcgg tgaggacgcg cacggcctcg gcgccgcgcc cattctccat caagcgtcgc
     7381 gccaaatttc cgacgagctc gaccgcgttc gaacgattcg tcacgggaac catcgaagcg
     7441 tcgggccgag gcgcggccgc gcccggaacc gcgacgcctc gcttcattgg ctcgcccgtg
     7501 tgatgctcga tggggatcgt cgccgaagct cggctgctga aaacactgac ctctgtcgtc
     7561 ccaatcagca gtcgatcgcc ctcggacaac cggtacgagg ggcgcgccag gcgcaccccg
     7621 ttcacgaata cgccgttcgt actgtgtaaa tcttcgacga cgaccgtctc gtccgcgagc
     7681 acgacgaggc gcgcgtgggc gcgcgagatc aacggatcgt ccagcaccac gttggcgtct
     7741 tccgctcgcc ccagcactag tccgccgcgc tcgtgttcga attcgccaac gggcgtcacc
     7801 accacgatcg gtgccttgag ccgatgcgca ggagggcggt cggaagtaat ggtccgctgc
     7861 atgggagcag gcgcgaccgg ggtcacacgg tgcctgttgt tcattgatgc cagctgaaat
     7921 cacagcattt ctctcgcgag ctcgctagcc agcgcgtctc gaaacggagc gaaatcagaa
     7981 gcgctcacgc gcgcacacct gtcggtacag cgcgccgccc ccgcacacct gcgtggagcg
     8041 aacttgccaa cgtcgaactc gcgagccgga cggttcgacg ggccgcacgc aagcctctgc
     8101 gccgcgcgcg ccgcgcaaat ttcgcgcgaa acacccggct ggccttcacc gcgagctccg
     8161 cagaaccgta gttcggggta gggactgatg ctgcctgaac gcaatttcgc gccctcgatg
     8221 gtcgaggtgt ccgtggtgct cgtcaacggt aagagccaca cgggactcct cgcgcgattc
     8281 aatccgatgg cacccgacgt cgcgctcagc ctggcgcaag acgccaaggg cagctcccga
     8341 tttgccgccg agcaggtcgc cttcgtcgcg ttccatcgcg cgccgggcga tcccccgccc
     8401 ccgcccacac gccgcggaac gctgaagatc cacgtgagcg gaggtaagac gttcgtcgtc
     8461 gacccagtcg acccgagccc gcccggtgca atcggcgctt acatgcgacc cgccgaggcg
     8521 cagagcccat accgcgagat cttcttttac aaccacggcg tgaacctgcg cgaggtcaac
     8581 gagcccatcg gcgtgctcct cgtgaaggaa ggccggctgg gcgcggaggc ggtgcaagcg
     8641 gggcttctcg cccaaaagga cgaaaagcga acgccgatcg gccagatcct catcgagaac
     8701 aagcgaatca accaagcggc gctcgaccag gcgacggcac tccagcaacg caaaggcacc
     8761 cgcattggtg aggtcctgat cgaagccggg ctcgcggacc aagccgacat cgactttgcg
     8821 ctgagtgagc agcgccgtcg aggaggcaag cgcatcggcc aaattctggt cgaaatgaat
     8881 ctcatcagcg aggtcgattt ggcggtcgcg ctggcgcgca agttccagct gccattcgtg
     8941 aacctcgacg aatgctccat caacgtggat gcggtcgccg agcttcctcg cgacttcgtg
     9001 gagaagaacc gcgtgctgcc ggtggagtcg gatccgcgct ccatcaccgt ggccctgagt
     9061 gatccgctcg cggtcgacac cctcgacctc gtccgcatcc actccaaaaa gcagctgagc
     9121 gaggtggtcg tcactcgcag ccagctcgac aagtacattc ccacctacct cgaccagctc
     9181 gaagccaaga accgcgccaa cgagatggac gagatcctga aaggcctcgc gagcggcgac
     9241 gcccaggcgg tcacccccga agaggcgacc gaagcgacgg tcaaggatac cgacaacgcg
     9301 atcatcaagc tcgcgaatca gatcatcatc gacgcttacc ggcgaggagc gtccgatatc
     9361 cacatcgagc cgaacgggcg cgagcgaatc acgaacgtcc ggttccgcgt cgatggcgat
     9421 tgcatcacct accaggacat cccgtccgct taccggctac cgctcgtcgc gcgcttcaag
     9481 atcatggcgc agctcgacat atcggagcgt cgaaagccgc aagacggcaa gatccgtttc
     9541 cgggtcggag agcggcaaat cgagcttcgt gtcgcgacca tccccacggt gaacaacaac
     9601 gaagacatcg tgctgcgcat cctcgcgggg tcgaagccgc tgccgctcga acaaatgggc
     9661 atgagcgcgc gaaacctcgc ggagtttcgc agcgcgatca agaagcctta cggcctcgtc
     9721 ttgtgcgtgg gaccgaccgg ctccggcaag acgaccacat tgcactcggc gctcggcgac
     9781 atcaacacgg tcgatacgaa gatctggacc gcggaagacc cagtcgaa
//
LOCUS       DASTKS010001270         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1767620, whole genome shotgun sequence.
ACCESSION   DASTKS010001270 DASTKS010000000
VERSION     DASTKS010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767620"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFQ35_27170"
     CDS             complement(<1..390)
                     /locus_tag="VFQ35_27170"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934426.1"
                     /translation="MSLAQLDLRHYRGLGAVRRVVRHARGLLLELDEEHFRADVLRSD
                     VLRLKVSHGGNFDEAPTYAACFDPNSAAPFEVFESDSRVELTTAALRLIIDKRDFSFD
                     VLRSDGSVVLESSRAEDGRALGFCQLND"
     gene            complement(430..1461)
                     /locus_tag="VFQ35_27175"
     CDS             complement(430..1461)
                     /locus_tag="VFQ35_27175"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9934427.1"
                     /translation="MGGSSSFARIRGVGLLITLAIGVAAAAGCKPKQTDGKASPAASG
                     SAAATGGPCEQWAQKVCAKAGAQSPACTSFTETTQLMSPATCTAGLKDIKVTLDKLSA
                     QRGECDKLVKTLCAAIGPETKTCQMVTTQTANFPPAQCKMMMGKLPEITEELKKMEAQ
                     NQPLSADLQAAIAANTAPAFGPENASVKIVEFSDFQCPYCAHAADVVHQIREKYGDKV
                     RFVFRQFPLPFHAQARGAAEAALAANAQGKFWEFHDRLFKNQQALEPAKFEGYAKDAG
                     LNVAQFKKALEDKKLGEQVEADVKLGEQVRVNGTPTMFVNGARVENPGDFNAVATMID
                     AALKGGTPG"
     gene            1715..2539
                     /locus_tag="VFQ35_27180"
     CDS             1715..2539
                     /locus_tag="VFQ35_27180"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET9934428.1"
                     /translation="MPLRYVEWTPLHSFSGGKLFVLQTYTDDIHRLATMLRAARRTVV
                     FTGAGMSTESGLPDFRSAGGLWKQNRRFEELASVDALERSYHEFVEFYRYRIRMLGNV
                     RPNPGHEILAGWQKSGLVQTLITQNVDGLHEDAGSSEVLSLHGNLRRIHCRLCAADMP
                     SSAFLEDIGTRCGRCRGAMRPSVVLYGETLDQRTMEAATSAAARADLFVVLGSSLSTA
                     PANGLPRIAIEQGGASLVIVNREPTPLSPLATLDVRGPIGNTLALVDQKVGGRRAR"
     gene            complement(2567..>2822)
                     /locus_tag="VFQ35_27185"
     CDS             complement(2567..>2822)
                     /locus_tag="VFQ35_27185"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9934429.1"
                     /translation="PNEVEEVVLQHPDVQEAGAVGVPDEKSGEVVKVVVVKKNQSLTA
                     EDLIHFCRERLAGYKVPKHVEFRAELPKTNVGKVLRRALR"
BASE COUNT          462 a          930 c          891 g          539 t
ORIGIN      
        1 gtcgttcagc tgacagaagc cgagcgcgcg accgtcctcg gcgcgagagc tctcgagcac
       61 caccgaacca tcgctgcgca ggacgtcgaa ggagaagtcg cgcttgtcga tgatcaaacg
      121 aagcgcggcg gtcgtcagct cgacgcgcga gtcgctctcg aaaacttcga acggcgccgc
      181 ggagttcggg tcgaagcagg cggcgtacgt cggtgcctcg tcgaagttac ctccgtgcga
      241 gactttcagg cgcagtacgt cggagcgcaa cacatccgcc cgaaagtgct cctcgtcgag
      301 ctcgaggagc agtccgcgcg cgtgacgaac gacgcgccga actgctccta gcccacgata
      361 gtggcgcaaa tcgagctgag ccaagctcat cgcgcgagac cgcgttgcga gcggctgcgg
      421 cgctccggct cagccgggcg tgccgccctt cagcgctgcg tcgatcatgg tggcgacggc
      481 gttgaagtcg ccggggttct ctactcgcgc gccgttcacg aacatggtgg gcgtgccgtt
      541 gacgcgcacc tgctcgccga gcttgacgtc tgcttcgacc tgctcaccga gcttcttgtc
      601 ttcgagcgcc ttcttgaact gcgcgacgtt gaggccggcg tccttcgcat agccctcgaa
      661 cttggccggc tcgagcgcct gctggttctt gaagaggcga tcgtggaact cccagaactt
      721 gccttgggcg ttcgcggcga gcgccgcctc tgccgcgccg cgggcttgcg catggaaggg
      781 gagggggaac tgacggaaca cgaagcggac cttgtcgccg tacttctcgc ggatctggtg
      841 caccacgtcg gccgcgtgcg cgcagtacgg gcactggaag tcggagaact cgacgatctt
      901 gacgctcgcg ttctcggggc cgaacgccgg cgcggtgttc gcggcgatgg cggcttgcag
      961 gtcagccgag agcggctggt tctgcgcctc catcttcttc agctcctccg tgatctccgg
     1021 gagcttgccc atcatcatct tgcactgagc gggcgggaag ttcgcggtct gagtcgtgac
     1081 catctggcac gtcttggtct cgggaccgat cgctgcgcaa agagtcttta cgagcttgtc
     1141 gcattcgccg cgctgcgccg acagcttgtc cagcgtgacc ttgatgtcct tcaggccagc
     1201 ggtacaggtc gccggtgaca tgagctgagt cgtctcggtg aacgaagtgc aagcggggga
     1261 ctgagcgccc gctttcgcgc agactttttg cgcccactgc tcgcagggcc caccagtcgc
     1321 cgctgccgaa cccgaagctg ccgggcttgc cttgccgtcg gtctgtttcg gcttacagcc
     1381 cgcggctgcc gcgacaccga tggccagcgt gataaggagg ccaactcctc ggatgcgcgc
     1441 gaaactcgat gaaccaccca tgatgcaatc cttgcttctg cgaacgactg ccgtccgaag
     1501 aggaggccgg tcttagcagc tctcccccgg aagggctatg ggcgcccgcc ctcagggctt
     1561 tttcggcgta gtttcgcgcc aagcggtctg acgcagcgcg gggctcatcc aggagagccg
     1621 agaaccgcga aaactttacg accgaactac tcgccgcgcg ggccgtacca atcgttcaac
     1681 cacgaggggt gaacgcttct agaagtgtcg ccgcgtgccg ttgaggtatg ttgaatggac
     1741 gccgctccat tccttctccg gcgggaagct cttcgtgctg caaacctaca ccgacgatat
     1801 ccatcgactc gcaaccatgc tccgcgcggc gcggcgcacc gtcgttttca ccggcgccgg
     1861 catgagcacc gagtctggtt tgcccgactt cagatctgcc ggaggcttgt ggaagcagaa
     1921 ccggcgcttc gaggagctcg catccgtgga tgcgctcgag cgttcgtacc acgagttcgt
     1981 cgagttctac cgctatcgca ttcgcatgct cggcaacgtg cggccgaacc ctggccacga
     2041 gatcctcgcc ggttggcaaa agagtggtct cgtacaaacc ctcatcactc agaacgtaga
     2101 cggattgcac gaggacgccg gcagcagcga ggtgctcagc ctgcatggaa acttgcgacg
     2161 cattcactgt cgcttgtgcg ctgccgacat gccctcctcg gcgttcctgg aggacatcgg
     2221 cacgcgctgt ggtcggtgtc gaggcgccat gcggccgtcg gtggtcctgt acggcgaaac
     2281 actcgaccag cgcacgatgg aagccgcgac gagcgctgcc gcgcgcgccg accttttcgt
     2341 cgttctgggc tcttcgcttt cgaccgcgcc ggcgaacggt ttgccgcgca tcgcgatcga
     2401 gcagggtggt gcgtcgctcg tcatcgtgaa ccgtgagcct acgccgctgt caccgctcgc
     2461 cacgctcgac gtacgtggcc ccatcggcaa tacgctcgcc ctcgtcgatc agaaggtcgg
     2521 agggcgccga gcgcgttgat tcgacgcccg gcgccagaga gtgcgctcag cgcagcgcgc
     2581 gccgcagcac tttgccgacg ttcgtctttg gcagctcggc cctgaactcg acgtgtttcg
     2641 ggaccttgta tcccgccaag cgttcgcgac aaaaatggat caggtcctcc gcggtcaggc
     2701 tctggttctt cttcaccacg acgaccttca ccacttcgcc cgacttctcg tcgggtacgc
     2761 cgaccgcgcc tgcctcttga acatcgggat gctgcagcac cacctcctcg acctcgttcg
     2821 gg
//
LOCUS       DASTKS010001271         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1322431, whole genome shotgun sequence.
ACCESSION   DASTKS010001271 DASTKS010000000
VERSION     DASTKS010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322431"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFQ35_27190"
     CDS             <1..635
                     /locus_tag="VFQ35_27190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934430.1"
                     /translation="AWPAPRVASIAEDAVAPKPTETRPLKRGEIHSRPLPVISVRRLL
                     RAALAHSSDANADERLQGLSSRMRAAAALPELSLRAARSTNESLRLSPNGTLVNDYTQ
                     TGGAGVIFEARATWRFDRLVFADDELHVERLRIQRERVRERVIELLLKHLFTWQRARS
                     RLLTGGDTLSDEERALGEAELDSARAALDVLTDGAFSDELPRLEAASDSP"
     gene            807..1941
                     /gene="prfB"
                     /locus_tag="VFQ35_27195"
     CDS             join(807..875,877..1941)
                     /gene="prfB"
                     /locus_tag="VFQ35_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146649532.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9934431.1"
                     /translation="MLSDTRSELDDLRRRVDALRGSLDVPQLKREIEELDSISSDPAF
                     WNNQEKAQAHMRRRAAAVEKLEHIEGLNRDVSDAIELLELGASENDEGTINEIGRQVP
                     SLAQRVRAAELRRMLSGPVDHANAIVSIHPGTGGTDAKDWAEMLLRMYLRWCERKGYK
                     TEVIDYQVSDEAGIDGASFTVSGPNAFGYLRAEGGVHRLVRISPFDGNARRQTSFAAI
                     EVTPDVEDEIDIDVKDTDLEVTTMRAGGKGGQNVNKVETAVRMRHLPTGIMVVCRAER
                     SQHQNRAMALKMLKARLYEMEEAKREAEQARYEAAKGQIAWGNQIRSYVLQPYQLVKD
                     LRTEHETSDVQAVLDGDLDPFIEAYLLQHADQKRENEHPNATV"
     gene            2069..>2851
                     /locus_tag="VFQ35_27200"
     CDS             2069..>2851
                     /locus_tag="VFQ35_27200"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9934432.1"
                     /translation="MESSEDALIQARRDKAARSREAGQDPFPNDFDASDRSEIAALKQ
                     RYAPALLEPVSEQRYSPELVQSVGGGEAVHLFGRLMARRGFGKASFLRVRDSSGEIQV
                     FAKQDVLGEYFARLEQIDIGDHVEVRGPMMVTKTGELTVEARFLRLAGKALRPLPDKW
                     HGLTDTDARYRRRYVDLVANPEVATVLRARSILVQALRSFLDGRGFLEVETPTLHTLI
                     GGAAARPFVTHHNTLDLELYMRVAPELYLKRLLVGGYERVYEI"
BASE COUNT          517 a          918 c          975 g          441 t
ORIGIN      
        1 tcgcttggcc ggcgcctcgc gtcgcgtcga tcgcggagga cgcggtcgct cccaaaccca
       61 ccgagacgcg tccgctgaag cggggggaga ttcactcgcg gccgctgccg gtgatcagcg
      121 ttcggcgact gttgcgcgcc gcactggcgc acagctcgga cgccaacgcg gacgagcgac
      181 tgcaggggct ctcctcgcgc atgcgcgctg cggccgcgct tccggagctc agcctgcgcg
      241 cggcgcggtc cacgaacgaa tcgctcaggc tctcgccgaa cggcacgctc gtgaacgact
      301 acacgcaaac cggcggagct ggagtcatct tcgaggctcg agcgacttgg cgcttcgatc
      361 gcctcgtgtt cgcggatgac gagcttcacg tcgagcggct tcggatccaa cgagagcggg
      421 tgcgcgagcg cgtcatcgag ctgctgttga agcacctgtt cacctggcag cgcgcgcgga
      481 gccgactctt gaccggcggc gatacgctca gcgacgagga gcgggcgctc ggcgaggccg
      541 agctcgacag cgcccgggcg gcgctcgacg tcttgaccga cggcgctttc agcgatgagc
      601 tgccgcgcct cgaggccgcg agtgactcgc catgacggcg cgagcgtaca cggcctggag
      661 gcgcgagcga ggcgccgcca cgtaggctcg cggcccaggg tggtaggctg gagcgcccgg
      721 acccgctcgg gcccggtaga cgccggaatc gccgagccgg ggctttggac tggcgatttt
      781 tcccagaaat tgtagaaccc cgcgccatgc tttccgatac gcgaagcgag ctcgatgacc
      841 tccgacggcg cgttgatgcg ctaagggggt ctctttgacg tccctcagct gaagcgcgag
      901 atcgaagagc tcgatagcat ttcgagcgac cccgcctttt ggaacaatca ggagaaggcg
      961 caagcgcaca tgcgccggcg tgccgccgcg gtcgaaaagc tcgagcacat cgagggattg
     1021 aaccgcgacg tcagcgacgc catcgagctc ttggagctcg gcgcgtcgga aaacgacgag
     1081 gggacgatca acgagattgg ccggcaggtc ccgtcgttgg ctcagcgtgt ccgcgcggcg
     1141 gagcttcggc gcatgctctc tggcccggtc gaccacgcga acgccatcgt gagcatccac
     1201 cccgggacgg gcggaaccga cgccaaggac tgggcggaaa tgttgctccg catgtacctg
     1261 cgctggtgtg agcgcaaggg ttacaaaacc gaggtcatcg actaccaggt gagcgacgag
     1321 gcggggatcg acggagcttc gttcacggtg agtgggccca acgccttcgg ctacctgcgc
     1381 gcggaaggcg gagtgcatcg cctggtacgc atcagcccct tcgacggtaa tgcgcgccgt
     1441 cagacgagct tcgccgccat cgaagtgacc cccgacgtcg aggacgaaat cgacatcgac
     1501 gtgaaagata ccgatctcga ggtcaccacg atgcgggcag gtggcaaagg cggtcaaaac
     1561 gtcaacaagg tcgagacggc cgtgcgaatg cgacatttgc caaccggcat catggtcgtc
     1621 tgccgcgcgg agcgtagcca gcatcaaaac cgcgccatgg ctctcaagat gttgaaggcg
     1681 cggctttacg agatggaaga agccaagcgc gaggccgaac aggcgcgcta cgaggccgcc
     1741 aagggccaaa tcgcctgggg caaccagatt cgcagctacg ttcttcagcc gtatcagctc
     1801 gtgaaggatc tgcgcaccga gcacgaaacg agcgacgtgc aggccgtgct cgacggcgat
     1861 ctcgacccgt tcatcgaggc ctatctgctg cagcacgcgg accagaagcg cgagaacgag
     1921 catccgaacg cgaccgtctg agagtcgggc gcgaagttgc gcgttggggc gggcagtcct
     1981 gctcgcccag cgccggcgcg agccgggcgc atccagggta gggaggtaga ctccccagcc
     2041 cggtcgcgct agacccccga gcgccacgat ggaaagcagc gaagatgcgc tgattcaggc
     2101 tcggcgcgac aaggcagctc gttcgcgcga ggctggacag gatccgtttc ccaatgactt
     2161 cgacgcgagc gatcgcagcg agatcgcggc gttgaagcag cgctacgccc ccgcgttgct
     2221 cgagccggtg agcgagcaac gctactcgcc ggagctcgtg cagagcgtcg ggggaggcga
     2281 ggcggtgcac ttgttcggtc gcctcatggc gcgacgcggc tttggcaagg cgagcttcct
     2341 gcgcgtgcgc gacagcagcg gcgagattca ggtcttcgcc aagcaagacg tcttgggcga
     2401 gtactttgcc cgcctcgagc agatcgacat cggtgaccac gtcgaggttc gcggtccgat
     2461 gatggtgacc aagaccggcg agctcactgt cgaggcgcgc ttcctacgcc tcgccggcaa
     2521 ggcgctgcgc ccgttgccgg acaagtggca cggcctcacc gataccgacg cgcgctatcg
     2581 acgacgctac gtcgatctcg tcgcgaaccc cgaggtcgcg acggtgctgc gcgctcgctc
     2641 gatcttggtg caagcgctgc ggagtttcct cgatgggcgc ggtttcttgg aggtggagac
     2701 ccccaccctg catacgctga tcggcggcgc cgccgcgcgc ccgttcgtca cgcatcacaa
     2761 cacgctcgat ctcgagctct acatgcgggt cgcgcccgag ctctacctca agcggctctt
     2821 ggtcggcggc tacgagcgcg tttacgagat c
//
LOCUS       DASTKS010001272         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1767956, whole genome shotgun sequence.
ACCESSION   DASTKS010001272 DASTKS010000000
VERSION     DASTKS010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767956"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1483
                     /locus_tag="VFQ35_27205"
     CDS             347..1483
                     /locus_tag="VFQ35_27205"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9934433.1"
                     /translation="MKSESQDAPEYSAGVKAGEWDDNANYREFMRWLQTRPAPSAHFV
                     DLRHRRFIVVRDAAGQGVPNCRVVIEDDADRRTELVTAATGRAVLFPHAEGLTGQTFT
                     ASTTCEGGAQVRFDLAEPDGVVALRLAGRRVLNARTIDVAFVLDSTGSMSEEIAAVKA
                     TIQKVATSLRRVNVRVRVGIVEYKDRSDTYVTKVYPLSSDLARFSTQVAALSASGGGD
                     TPESVNEALHVAVEQLSWSDESFARLAFLVGDAPPHLDYPQDYDYILEAREAAHRGIQ
                     VFTVAASGMDTLGQVVWRQVAAYTGATNLFVLRGGAGPQSVGAGDPKSSCGGTQTAYT
                     SGNLDALVLAKIQGAVRALERDPMRIPGLNADENSKPCANRIAQ"
     gene            complement(1506..>2052)
                     /locus_tag="VFQ35_27210"
     CDS             complement(1506..>2052)
                     /locus_tag="VFQ35_27210"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET9934434.1"
                     /translation="LDRISTYMRLLGVPAQRKYASGYTDGTVTPPEHDLDDATIATIA
                     RGSRLFAEAACTACHVAQIKTGANHPFYELRNQVIRPYTDLLLHDMGPGLADTMTEGQ
                     ASPSMWRTPPLWGLGSLKYVQKQTGAADPQSVRYLHDGRARTLLEAIVWHDGEALGSR
                     NKFEALSRDDRAALIRFLESL"
BASE COUNT          353 a          645 c          691 g          363 t
ORIGIN      
        1 tctgctgacg atgacgcatg ctcgcgagga cgcgggggtt gcggtggtgc cgagcgaagc
       61 ggttgattcg gcagggccga ctcagcaacc tcctgccgtc gtcgaagcgc cgcccgttgc
      121 tgtcccggcg ccgcctgccg agggttatgc gttggcacca agccctcgat ccatgggcgc
      181 tccgcgaccg agtcgcgcgc cgtcggcctc aaatgggcca cccccgcaca agtgggattg
      241 gagggagaac cctggcatcg aatcgggccg cgtcgctggg gccgtagggt ccgacgatcg
      301 tccccacccc tcgcaggccg aagcgcccct ccctcgcgct cctctcgtga agagtgagtc
      361 gcaggatgca ccggaatact cggcgggcgt caaggccggc gagtgggacg ataacgcgaa
      421 ctaccgcgag ttcatgcgct ggctccaaac caggcccgca ccctccgcgc acttcgtcga
      481 cttgcgtcac cggcggttca tcgtcgtccg cgacgcggcc gggcaggggg ttccgaattg
      541 tcgcgtggtg atcgaagacg acgccgatcg ccgaacggag ttggtcacgg ccgcgaccgg
      601 tcgcgccgtc ttgtttcctc acgccgaggg attgacgggg caaacgttca ccgcgtccac
      661 gacttgcgaa gggggagcgc aggtacgatt cgatttggcc gagcctgacg gagtcgtcgc
      721 gctgcgcctc gcgggccgcc gagtgctgaa cgcgcgaacc atcgacgtcg ccttcgtctt
      781 ggactcgacc ggctcgatga gcgaagaaat tgcggccgtc aaagcgacca ttcaaaaagt
      841 ggcaacttcg ctccgacgag tgaatgtgcg cgttcgagtg ggtatcgtcg aatacaagga
      901 tcggagcgat acgtacgtga ccaaggtcta cccgttgtcg agcgacctcg cgcggttttc
      961 gactcaggtc gcggcgttgt cggcgagcgg tggcggcgat acgcccgagt cagtcaacga
     1021 agcactgcat gtcgcggtcg agcagctatc gtggagcgac gagtcgttcg ccaggctcgc
     1081 gtttctcgtg ggcgacgcgc ctccgcacct cgactaccca caagactacg actacatcct
     1141 cgaggcacgc gaggcggctc atcgcggcat tcaggtgttc accgtcgccg catcggggat
     1201 ggacaccctc gggcaagtcg tttggcgcca agtcgcggcg tacacgggcg caacgaacct
     1261 attcgtgttg cgtggtggag cggggccaca atcggtaggc gcgggtgatc ccaaatcgag
     1321 ttgcggcggc acccaaaccg cctacacgag tggcaatctc gacgccctcg tcctcgcgaa
     1381 gatccagggc gcggtccgcg cgctcgagcg agacccgatg cgaattccag ggctgaacgc
     1441 cgacgagaac tcgaagccct gtgcgaaccg catcgcacag taggggagtg agacgctctc
     1501 ggctgctaca gcgactccag aaacctgatg agcgcggcgc gatcgtctct gctcagcgct
     1561 tcgaacttgt tgcgactgcc gagtgcttca ccgtcgtgcc agacgatcgc ctcgaggagc
     1621 gtgcgcgcgc ggccgtcgtg taggtagcga acgctctgtg gatctgcagc gcccgtctgc
     1681 ttttgcacgt acttgagcga accgagcccc cacagcggcg gcgtacgcca catgctcggg
     1741 ctggcttggc cttcggtcat cgtgtcggcg aggccgggcc ccatgtcgtg aagcaacaga
     1801 tccgtataag gtcggattac ctgatttctg agctcgtaga aggggtggtt cgcgccggtc
     1861 ttgatttggg cgacgtggca ggccgtgcac gccgcttctg cgaacaaccg actaccgcga
     1921 gcgatggtcg cgatggtggc gtcgtcgagg tcgtgctcgg gcggagtcac cgtgccgtcg
     1981 gtgtagccgc tcgcgtattt gcgctgagcg gggacgccca gcaatcgcat gtaggtagag
     2041 atgcgatcga gc
//
LOCUS       DASTKS010001273         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1546234, whole genome shotgun sequence.
ACCESSION   DASTKS010001273 DASTKS010000000
VERSION     DASTKS010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546234"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1374)
                     /locus_tag="VFQ35_27215"
     CDS             complement(190..1374)
                     /locus_tag="VFQ35_27215"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9934435.1"
                     /translation="MAASGASIISGLEELISRGDFVVPPYPAAALRLRRLVNSQNFGL
                     AEVAEAAASDPALAATLLRLANAATYRGSGPAITSLHRAVHRLGAKSVATLALTAGIS
                     KAALDQGPLVDVKYRVWRRSVTCALCAQKLASLRGVDPEEAFLGGLLYGFGRSVAVGC
                     LERVLPPGPTQARPLLDWLAAVEPHRHALAKRVAQSWQLPSELVEVLTGASTKPELKV
                     LIGLLELSDRIAGALDGGATADLAATLVGATEKETRAVAHFIADLPHTLETLLEAPDP
                     ARRLDSVNAVAKPDTALSGEARPLALGLSDLKTRKEPRPLQAVAVTPDGLVFLSDRPM
                     QESCVARLGLVIGSTVLEGWFSVSLCVRERDHYRVEAQMFAPSRELKEQLAELWSSAS
                     RS"
     gene            1545..>2243
                     /locus_tag="VFQ35_27220"
     CDS             1545..>2243
                     /locus_tag="VFQ35_27220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9934436.1"
                     /translation="MRFAISVPWLGRLATCLDADNAKRAIEAATELLAEVGALPVLSG
                     EPPALSVEYAGHRLAFSRGALDDETLVGAKAMLRAALRRVAEQDALESARERMNMLAS
                     ASFEGIFIHVDGSVIDANPRFCEMLGRSYEEILGAETLRRCVAPEDLPLVLELVRNRY
                     EGAYVVTGVRADGSRFRAELLSKQGKLGARPVRVVAVRDVTDRERTHALLSESESRLS
                     ELAEEVFDVIVLSRG"
BASE COUNT          370 a          716 c          778 g          379 t
ORIGIN      
        1 gtgacgaccc cacattcggc ccgcgtgcgc agcccaagct gctcagcgca atgctcgccc
       61 aaacgatgcc tcgcatagac ttctcctcgt gccgaaagcg agggtgcttt cggcgcacgg
      121 gagcgtaccc gaggccgagg cggcgttaca gagggtggcg gcagggtggt gagtgggccg
      181 gggctcgact caactgcgtg acgccgaact ccagagctcc gcgagctgct ctttcagctc
      241 gcgactcggc gcgaacatct gcgcttcgac tcggtaatgg tcgcgctcgc gcacgcagag
      301 cgaaacgctg aaccagcctt cgagcaccgt gctgccgatc accaggccga gccgggccac
      361 gcagctctcc tgcatcgggc gatcgctcaa gaaaaccagc ccgtcggggg tcaccgcgac
      421 ggcttgaaga ggtcggggct ctttccgcgt tttgagatcg ctgagcccca gcgcgagcgg
      481 ccgagcctct ccgctgagag cggtatcggg tttggccacc gcgttgaccg agtcgaggcg
      541 tcgcgcgggg tccggagctt cgagcagggt ttcgagggtg tggggaagat ccgcgatgaa
      601 gtgcgcgacg gcgcgcgttt ccttttcggt cgccccgacc aaggtcgcag caaggtctgc
      661 cgtcgctccc ccgtcgagcg cgccggcgat gcggtcggag agctcgagta agccgattag
      721 cactttcagc tcgggtttgg tcgatgcgcc cgtcaagact tcgacgagct cactgggcaa
      781 ctgccagctc tgtgcgactc gtttcgccag ggcatggcgg tgcggctcga ctgccgcgag
      841 ccaatcgagg aggggacgag cctgagtcgg gcccggcgga aggactcgtt ccagacaacc
      901 gacagcgacc gaacggccga acccgtagag cagcccgccc aagaaggctt cttccggatc
      961 gacgccgcgc aggctcgcga gcttctgcgc gcagagagcg cacgttacgg agcggcgcca
     1021 aacgcggtac ttcacgtcga cgagcggtcc ctggtcgagc gcggccttgc tgattccggc
     1081 cgtgagcgcg agcgtcgcga ccgacttggc gccgaggcga tggaccgcgc ggtgcagcga
     1141 agtgatcgcc gggccgctcc cgcggtaggt cgcagcgttc gcgaggcgga gcagcgtcgc
     1201 ggcgagcgcc ggatcgctcg cggctgcctc cgcgacctcg gcgagcccga aattctggct
     1261 gttcacgagg cgccggagcc gcagcgccgc cgcgggatac ggcggaacga cgaagtcacc
     1321 tcgcgagatg agttcttcga gaccggaaat gatgctcgcg ccgctggcag ccatttctct
     1381 cgcctcaatt acggctgaac tggcgtcgaa cgtaagtcgc ttggatgacg aaactcaaca
     1441 cgtgcaccta caccgtcagg cgagttcaga cttccgggta gaactcctcg ttcggttccc
     1501 ctgaaggtcg gttagactgc gctcgacctc ccgctgcgtg acgaatgcgc ttcgcaatct
     1561 cggttccatg gctcgggcgt ctagcaacct gcctggacgc agataacgcg aagcgcgcga
     1621 tcgaagccgc cacggagctg ctcgcggagg tgggggcgct gccagtcttg tccggggaac
     1681 caccggcgct ttcggtcgag tacgccgggc atcggttggc tttctcgcgg ggcgcgctcg
     1741 acgacgagac tctcgtcggc gcgaaggcga tgttgcgagc ggcgcttcgt cgcgtcgccg
     1801 agcaagacgc gctcgagagc gcgcgcgagc gcatgaacat gctcgctagt gcctctttcg
     1861 aggggatttt cattcacgtc gacggctcgg tcatcgatgc gaacccgcgc ttctgcgaga
     1921 tgctcggccg ttcgtacgaa gagatcctcg gcgcggaaac tttgcgacgc tgcgtcgccc
     1981 cggaggatct gccgctcgtc ttggagctcg tgcgaaatcg ttacgagggc gcgtacgtgg
     2041 tcaccggcgt tcgcgcagac ggcagtcggt ttcgcgccga gctgctctcg aagcagggca
     2101 agctgggggc gcgtcccgtg cgcgtcgtgg cggtgcgcga cgtgaccgac cgcgagcgta
     2161 cgcacgctct gttgagcgag agcgaaagcc ggctgagcga actcgccgaa gaagtgttcg
     2221 acgtcatcgt gctgagtcgc ggg
//
LOCUS       DASTKS010001274         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1768230, whole genome shotgun sequence.
ACCESSION   DASTKS010001274 DASTKS010000000
VERSION     DASTKS010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768230"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1534
                     /locus_tag="VFQ35_27225"
     CDS             164..1534
                     /locus_tag="VFQ35_27225"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HET9934437.1"
                     /translation="MGTRSHRAARCRRCRVHIESCVCAELPCIELATRVVLVMHQREA
                     SKTTATGPLALACLPNSAQYLHGLEGAPLDLTPLDENGARRLLVLFPTEGARTLSAAL
                     RDEDERPITLVVPDGNWRQARRIPQRVRGLEHAEHVGLPLGAPSAWGVRWEPMEWGLA
                     TFEAIARAIGVLESADAQARLEAAFARVVALTLAARGRDSVASEPVRAELRVSPVLEP
                     DTLEIVYRDEHLVAVNKPPGVPSHHGWSRDVRPALQRVRDQIGQPVFPVHRLDRATSG
                     VLVFALSSEVARDMQRILVASDKRYIALCRGHDSQLTRVDHPLAKAHGAEPREAVTEF
                     RLLGSFERYGLYEARPRTGRTHQIRRHLKHVSQPIVGDVRYGKGDHNRLFRERFGFHR
                     LALHCHEIRFDHPRGARSVRLHAALSPGFARLLEAIGLSSLVCSAAVEGSCALECAAR
                     VARDAR"
     gene            complement(1658..2518)
                     /locus_tag="VFQ35_27230"
     CDS             complement(1658..2518)
                     /locus_tag="VFQ35_27230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934438.1"
                     /translation="MLRAVNRRAHSPRAWLLSLLLVLPIASNSTAHATDRASDRADRR
                     EGRADARDDSRAEQREWCSPEVETLWDGMCFHAPDEAATAEHPTLVLFLHSLVAVKSD
                     WQWEQQRELARAADRHGFAVLLPRGRTGIGPRRSGDVWAWPTSPASQADVEDELISEW
                     RRARRTIESRNGHPFERLLVFGFSNGAYYASTLALRNRLDAHGYGVFAGGSGGKYASV
                     LGSRTTHRAPIFVGYGTKDPAWHDMASLADTLKSLGWRHQVKAEPVGHLVTDAQIAAA
                     IRFLGSHHPE"
     gene            2672..>3089
                     /locus_tag="VFQ35_27235"
     CDS             2672..>3089
                     /locus_tag="VFQ35_27235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934439.1"
                     /translation="MTLQLPNLSLSRYIARGALLLAFTVGAAALAGGCSPEPCSYSKD
                     VSVSPETTPFSDGSTVFSKCGACPELPKMPGVDASGPATVCRVAFHDSEPDSAWVDCF
                     YGPGGNTSSSIANGAVKDVPNLFAYCESQCPDEDAVH"
BASE COUNT          508 a          935 c         1055 g          591 t
ORIGIN      
        1 ggtggtgatg tgatgggagt cggctcgttc gatggcgctc ggggccgtac ggcgcggtcg
       61 ggcgtgaggc tgccgagagc gtggcgccag ttgtcgccgc gagcgacgca tcgagagggt
      121 cggctaggta ggcaggcgtg ggagcgctat gctctgggcg ctcatgggta cacgttccca
      181 tcgcgcagcg cgttgtaggc ggtgtcgggt tcacatcgag agttgcgtat gcgccgagtt
      241 gccttgtatc gagctagcca cgcgcgtcgt gctcgtcatg catcagcgcg aggcgagcaa
      301 gacgacagcg accgggccgc tcgcgctcgc ctgcctgccg aacagcgcgc agtacctgca
      361 cggtctggaa ggtgcgccgc tcgaccttac gcctctcgat gagaacggcg ctcgccggct
      421 cctcgtgctc tttccgactg aaggcgcacg cacgttgagc gcagcattga gggatgaaga
      481 cgagcggccg atcacgctcg tggttccgga tggcaattgg cggcaagcgc ggcggatccc
      541 acagcgggtg cgcggcctcg agcacgccga gcacgttgga ttgccgctcg gtgcgccgag
      601 tgcttggggt gttcgttggg agccgatgga atgggggctc gcgacgttcg aagcgatcgc
      661 gcgcgctatc ggtgtgctgg agtctgccga tgcacaggcg cgcctcgaag cggcgtttgc
      721 gcgtgtcgtc gcgctcacgc tcgcagcgcg cggtcgggat agcgttgcga gcgagcccgt
      781 ccgcgcggaa ctgcgtgtgt cgcccgtcct ggaaccagac acgctcgaga tcgtttatcg
      841 cgacgagcac ctggtcgcgg tgaacaagcc gcctggcgtt ccgtcgcatc atggctggtc
      901 gcgtgacgtg cgtcccgctc tgcagcgcgt tcgcgatcag attggacaac cagtgttccc
      961 ggtccatcgg ctcgatcgcg cgaccagcgg ggttttggtg ttcgctctca gctcggaggt
     1021 cgcgcgagac atgcaacgca ttctggttgc gagcgacaag cgctacatcg cgctttgccg
     1081 tggccacgat tcgcagctga cgcgggtcga tcatccgctt gcgaaagcgc atggagccga
     1141 accgcgcgaa gcggtgacgg agtttcgctt gctcggcagc ttcgagcgct acgggttgta
     1201 cgaggcccgg ccgaggactg gtcgaaccca ccagatccgc cggcatctca agcatgtttc
     1261 gcaaccgata gtcggcgatg tgcgctacgg caaaggagat cataaccggc tcttccgcga
     1321 gcgctttggc ttccaccgcc tagcgctgca ctgccatgaa atcaggttcg atcatccgcg
     1381 cggagctagg tccgtgcgcc tgcacgcggc tctcagcccg ggctttgccc gtttgctcga
     1441 ggcaattggg ctgagctcgt tagtctgcag cgctgcggtc gaaggctctt gcgcgcttga
     1501 atgtgctgct cgggtcgcca gagacgctcg atagcgcctg gaggccaggt tcacggtaac
     1561 cgtaacgcca aactcctaca gaggcggggc ggttaccttg cagcgacggc gcgagctcgc
     1621 agtttttcgc ggcttgccac cgagggtttg cgcggcgtca ctcggggtga tgggagccca
     1681 ggaaccgaat tgcggctgcg atttgcgcgt cggtgacgag gtggccaacg ggctcagctt
     1741 tgacttggtg acgccagccg aggctcttca gagtgtcggc gagcgaggcc atgtcgtgcc
     1801 acgcggggtc tttggttccg tagcccacga agatgggcgc gcggtgggtg gtgcggctac
     1861 cgaggacaga ggcgtacttg ccgccgctgc cccccgcgaa gacgccgtag ccatgagcgt
     1921 ccaatcgatt gcgtagagca agggtcgacg catagtacgc gccattggag aacccgaaga
     1981 cgagcaatcg ctcgaaaggg tggccgttgc gcgactcgat tgttctgcgc gcgcgtcgcc
     2041 actccgagat gagttcgtct tccacgtcgg cttgcgacgc gggcgaggtt ggccaggccc
     2101 agacatcgcc tgaacggcgc ggtccaattc cggtgcggcc gcgcggcaac aagaccgcaa
     2161 agccatggcg atcggccgct ctcgctagtt cgcgctgctg ttcccactgc cagtccgatt
     2221 tgactgcgac caggctgtgt agaaaaagga ccagagtcgg atgttctgcc gtcgccgctt
     2281 cgtccggagc gtgaaagcac atgccgtccc agagcgtttc gacttcgggt gagcaccact
     2341 cgcgctgttc cgcgcggcta tcatcgcgag cgtcggcgcg gccttccctg cgatctgcgc
     2401 gatcgctcgc acgatccgtc gcgtgagcgg tcgaattcga tgcaatcggc agcacgagta
     2461 gcagcgacag aagccaggcg cgtggagaat gagcgcgtcg gttcacagcg cgcaacatgc
     2521 cccaaagtgg cgcgcggagg acgcgaaatc cgcttgcagg cggggatttg agtcgcagtt
     2581 gcggcgaaag ttccgctgta agcgatcact catgccaatc taatacgcgt tggatgccgg
     2641 cgtcggcacg cgggttgctg tggggagccg catgacgctt caactgccga acttgtcgct
     2701 gtcacgctac attgcgcgtg gagcgctgtt actcgccttc acagtgggcg cagccgcatt
     2761 ggccggaggc tgttctcccg agccttgcag ctatagcaag gacgtttccg tctctcccga
     2821 aacgacgccg ttcagcgacg gctccaccgt gttttcgaaa tgcggagctt gcccggaatt
     2881 gcccaagatg cctggcgtgg acgcgagcgg accggcgacg gtgtgtcgcg ttgccttcca
     2941 cgactcggag cccgactctg catgggtaga ttgcttttac ggtcctggcg ggaatacgtc
     3001 ttcttctatc gcgaacggcg ccgtgaaaga cgtgccgaat ctattcgcct actgcgagtc
     3061 ccagtgtccc gacgaagatg cagtccacg
//
LOCUS       DASTKS010001275         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_650912, whole genome shotgun sequence.
ACCESSION   DASTKS010001275 DASTKS010000000
VERSION     DASTKS010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650912"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFQ35_27240"
     CDS             complement(<1..199)
                     /locus_tag="VFQ35_27240"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9934440.1"
                     /translation="MLAGKRIVVTGASGGIGRSIAQRAAKLGALVGVHGHTNRDASAN
                     LALELGGVSLAFDVRDGASVAR"
     gene            complement(186..1424)
                     /locus_tag="VFQ35_27245"
     CDS             complement(186..1424)
                     /locus_tag="VFQ35_27245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET9934441.1"
                     /translation="MTRRRVAITGMGVVAPNALAVPEFLDALLAGRSGIGPITVFDPA
                     SLPTRIAGEVKQTFPSPLPDRKIAFALEAARQAMTQATRSGTRPEGRGGLSLGVGLEL
                     FSMEDLAAREHGATLPREDRALRLSFMQTPSDLCAHFLSHAHGLTLAPRVHVSACAAG
                     TDALGHAFRLVRSGRADFVLAGGTDSMINPLGVAGFSTLNATSTRNTEPERASRPFDR
                     GRDGFVLGEGAAFLVLEPWEAALARGATALGELVGYGNSLDAHGISEPHPEGRGAAQA
                     MQRALDDAELSPAQISAINAHGTATPKNDPVETLAIHRVFGEHARRVPVMATKSMIGH
                     LISAAGAVEAVAALACMNAGWLHPTLNLEEPDPSCDLDYVPHVARRMAQSHVLSASYG
                     FGGHNAAIVLRGAEAARARG"
     gene            complement(1421..2293)
                     /locus_tag="VFQ35_27250"
     CDS             complement(1421..2293)
                     /locus_tag="VFQ35_27250"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9934442.1"
                     /translation="MIVATGAAGLVSAGEVDLDPSSFLRARKSRKFMGIQDDLAVITA
                     GRALAQARLSRELLGERAGLFAVIGYIPFDKSDIAPVLAASLEGERFSMQRFSNGGFQ
                     QAHPLLTFRCLPNMPAYHVSANFDVRGPYYVTYPGAGQVYAALEEASTALGEGLIDVA
                     LVFAVAHQRNFLVEHHLSRVDPPVPSAALRDAGGCLVLEREESARTAGRKAVLALLEM
                     EQRYRSYAPIEEQRGEVETFSGRAHASEEYRGPAALFCELADAVSSQKPLRVEHRLSA
                     RDGIDAFSTWQVHP"
     gene            complement(2290..2754)
                     /locus_tag="VFQ35_27255"
     CDS             complement(2290..2754)
                     /locus_tag="VFQ35_27255"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HET9934443.1"
                     /translation="MRYFLLDKVTAYEAGRMATGIKNVTLTDEVLHDHFPDYPVLPGA
                     LVLEGMAQLAGFMLEVGKPAPVLRAVLAQIEKAKFHSPAGPGDQVSMRVSLAHELETA
                     AQVDAEAYVSDRCIARARLTFMLRRIESPRVHEQRERLYALWTRDLRGETHP"
     gene            complement(2751..3770)
                     /locus_tag="VFQ35_27260"
     CDS             complement(2751..3770)
                     /locus_tag="VFQ35_27260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9934444.1"
                     /translation="MNLGVLKKDLSFALGILQKKPFQVLVQVTNRCNMRCSFCDFWPN
                     PAPKGQELSVSDYQRIARELAELGCFLISIEGGEPLVRKDLEGIIAAFSEHHIPALFT
                     NGWYVDEARARSLFGAGLVHASVSIDYASAAKHDAKRGLAETTARAWDAVDTFRRVAP
                     RGGLQVHVMTVLMRSNEDELEALLEQSRDRGVGHQFTLLATNGYRRGKSDVDELPTAA
                     ALQSFEQLFQRFPHIRFFGRYFQGAADFVQNRPLPTCRAGLQSFNIDHVGNVSACIER
                     IEETVGNVKEVAVPELHRRLSTRHHEIAQCQRCYTACRGFNQALGDGGSLESWVELGT
                     RMRSS"
     gene            complement(3767..>4616)
                     /locus_tag="VFQ35_27265"
     CDS             complement(3767..>4616)
                     /locus_tag="VFQ35_27265"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9934445.1"
                     /translation="FGDLEALKRSLAENRAAAFVVEPIQAEGGVIMPPVNYLAEARAL
                     CKRRGTLFVLDEVQTGLGRTGSLFAYESAGVLPDALVLGKSLGAGMVALSATVTSREL
                     WQKAYGSPEKFDMHGSTYSGNAFACRVGTEVLGELRTGDLVEATRVRGASFLEALRRA
                     LLGHPLVRDVRGAGLLVGIELGPTQSTLSQRVLPAMYESISQKVFGQWLALRLLERGI
                     VCQPASQHWNVLRLEPPLTVTDEQLGRTVTEISALLAEYTSLGPLMKDVAQRLGEQYR
                     GGWAFR"
BASE COUNT          770 a         1505 c         1545 g          796 t
ORIGIN      
        1 cacgcgcgac gctggcgccg tcacgcacgt cgaacgccaa cgacacaccg ccaagttcga
       61 gcgcgaggtt ggcggacgcg tcgcgatttg tatgtccgtg cacgccgacc aatgcgccga
      121 gctttgccgc gcgctgggcg atggagcggc cgataccgcc gctcgcgccg gtgacgacga
      181 tgcgcttacc cgcgagcacg cgctgcctcc gcgccgcgca gcacgatggc tgcgttgtgc
      241 ccgccgaatc catacgacgc gctcagaacg tgagattgcg ccatccgtcg cgcgacgtgg
      301 ggaacgtagt cgaggtcgca gctcggatcg ggctcctcga gattcaacgt tgggtgcagc
      361 cagccggcgt tcatgcaggc gagcgcggcg actgcctcga ccgcaccggc ggcggagatg
      421 aggtgaccaa tcatgctctt ggtggccatc accggcactc tccgagcgtg ctcgccgaac
      481 actcggtgaa tcgcgagagt ttctaccgga tcgttctttg gcgtggcggt tccgtgagcg
      541 ttgatggctg aaatttgcgc gggcgagagc tcggcgtcat cgagagcgcg ctgcattgct
      601 tgtgcggccc ctcgaccctc cgggtggggt tcgctgatgc cgtgcgcatc gagcgagttg
      661 ccgtaaccga cgagctcgcc cagcgccgtc gccccccggg caagcgcggc ctcccagggt
      721 tcgagcacga gaaacgccgc gccctcgccg agcacgaagc catcgcgacc gcggtcgaag
      781 ggacgcgatg cgcgctctgg ctcggtgttc cgggtgctgg tcgcgttcag cgtggaaaag
      841 ccggcgacgc ccaaggggtt gatcatcgaa tcggttccgc ccgccaggac gaagtcggcg
      901 cgcccgcttc gcaccagccg aaaagcgtgc cccaacgcgt cggttcccgc cgcgcatgcg
      961 gaaacgtgga cgcgcggagc cagcgtgagc ccgtgcgcgt gactcaagaa gtgcgcgcag
     1021 aggtcggacg gcgtctgcat gaacgacagg cggagcgcgc gatcctcccg cggtagcgtc
     1081 gcgccgtgct cgcgcgccgc gagatcctcc atcgagaaga gctcgagccc cacgccgagg
     1141 ctcagcccac ctcggccctc ggggcgtgtg ccggagcgcg ttgcttgtgt catcgcttgt
     1201 cgagcggctt cgagggcgaa agcgatcttg cgatcgggaa gcgggctcgg gaaggtctgc
     1261 ttcacctccc cggctatgcg cgtgggcaga gacgcggggt cgaacaccgt gatcggaccg
     1321 atgccggaac gtcccgcgag cagcgcgtca aggaattcgg ggacggcgag cgcgttgggc
     1381 gccacgactc ccatccccgt aatcgccacg cgacggcgtg tcacgggtgc acctgccagg
     1441 tgctgaaggc gtcgatgcca tcgcgcgcgc tgagccggtg ttcgacccga agcggctttt
     1501 ggctcgatac cgcgtccgcg agctcgcaga acaaggcggc ggggccgcgg tactcttcgg
     1561 atgcgtgggc gcgcccggag aaggtctcga cttcccctcg ctgctcttcg atgggcgcgt
     1621 atgagcgata gcgctgttcc atttcgagta aggcgagcac cgctttgcgg cccgcggtgc
     1681 gcgcgctctc ttctctctcg agcacgagac aaccccccgc gtcgcgcaac gcggccgacg
     1741 ggaccggtgg atcgacgcgg ctcaggtgat gctccaccaa gaagtttcgt tgatgcgcga
     1801 ccgcgaagac cagcgccaca tcgatgagac cttccccgag cgcggtcgag gcctcttcga
     1861 gtgccgcgta aacttgtccc gcgccggggt acgtcacgta gtaggggccg cgcacgtcga
     1921 agttcgcgga gacgtggtac gcaggcatgt tcgggaggca acgaaacgtg agcagcgggt
     1981 gcgcttgctg aaagcccccg ttggaaaacc gctgcatact gaaccgctcg ccttccagcg
     2041 atgccgcgag caccggcgcg atgtcggatt tgtcgaaggg gatgtacccg atcaccgcga
     2101 agagcccggc tcgctcaccg agcagctccc gcgacaggcg agcctgagcg agcgctcgcc
     2161 cggccgtgat caccgcgaga tcgtcctgaa tgcccatgaa tttgcgggat ttgcgcgctc
     2221 tcaaaaacga gctcgggtcc aggtcgacct cgcccgcgct gacgagccca gccgcgccgg
     2281 tggcgacgat cacggatggg tctccccacg tagatcgcgc gtccagagag cgtacaagcg
     2341 ttcacgttgc tcgtgcacgc gcggcgattc gatgcggcgg agcatgaagg tgagccgcgc
     2401 tcgcgcgatg cagcggtcgc tcacgtaagc ttcggcgtcg acctgggccg ccgtctcgag
     2461 ctcgtgggcg aggctcactc gcatcgagac ctggtcgccg ggccccgccg gtgagtgaaa
     2521 ctttgccttt tcgatttgag cgagcacggc tcgcagtacc ggcgctggct ttccgacctc
     2581 gagcatgaaa ccggcgagct gcgccatccc ttcgagcacg agcgcgcccg gtagcactgg
     2641 gtagtcgggg aagtgatcgt gcagcacttc atcggtcagg gtgacgttct tgatgccggt
     2701 cgccattctg ccggcttcgt aagccgttac cttgtccagc aagaagtacc tcacgatgac
     2761 ctcatgcgtg ttccgagctc gacccagctc tcgaggctgc cgccgtcgcc gagcgcttgg
     2821 ttgaagccgc ggcacgcggt gtaacaacgc tggcactgcg cgatctcgtg gtgtcgcgtg
     2881 gagagccgac ggtgtagctc gggcacggcg acctccttca cgttgccgac cgtttcctcg
     2941 atgcgctcga tgcacgccga cacgttgccc acgtgatcga tgttgaagct ttgaaggccg
     3001 gcgcggcacg tcggcagggg ccgattttgc acgaaatctg cggccccctg aaagtagcgg
     3061 ccaaagaagc gaatgtgagg aaatcgctgg aagagctgct cgaagctctg cagcgcggca
     3121 gcagtgggaa gctcatcgac gtcgctcttt ccgcgtcggt agccgttggt cgcgaggagc
     3181 gtgaattgat gccccacgcc gcgatctcgg ctctgctcga gcagcgcttc gagctcgtcc
     3241 tcgttgcttc gcatcagcac cgtcatgacg tgcacttgaa ggccgccgcg cggcgcgact
     3301 cggcggaacg tgtcgacggc atcccaggcg cgggccgtcg tttcggcgag gccgcgcttt
     3361 gcgtcatgct ttgcggcgct cgcatagtcg atcgagacgc tcgcgtgcac gagccctgcg
     3421 ccgaacagcg agcgagcacg cgcctcgtcg acgtaccagc cgttcgtgaa caacgcggga
     3481 atgtggtgct ccgaaaaagc cgcgatgatg ccctctagat ctttgcgtac cagaggctct
     3541 cccccttcga ttgaaatcaa gaagcaaccg agctccgcga gctcgcgcgc gatgcgctgg
     3601 tagtccgaga ccgagagctc ctgacctttc ggcgcagggt ttggccaaaa gtcgcagaaa
     3661 ctgcagcgca tgttgcagcg gttcgtgacc tgcacgagca cttgaaacgg ttttttctgc
     3721 aggatcccga gcgcaaagct caaatccttc ttcaagacgc cgaggttcat cggaaagccc
     3781 agccccctcg gtactgctct ccgagacgct gcgcgacgtc cttcatgagc gggccgaggc
     3841 tcgtgtactc cgcgagcagc gccgagattt cggtgacggt gcggccgagt tgctcgtcgg
     3901 ttacggtgag cggcggctcg aggcggagga cgttccagtg ctgcgaagcc ggttggcata
     3961 cgatgccgcg ttcgagcagc cgaagcgcta gccactggcc gaataccttc tggctgatgc
     4021 tctcgtacat cgccggtagc acgcgttggc tcagggtgct ctgggtcggc ccgagctcga
     4081 tgcccaccaa gagcccggca ccgcggacgt ccctgacgag gggatggccc agcagtgctc
     4141 gccggagcgc ttcgagaaag ctcgcgcccc gcacgcgcgt ggcttcgacg aggtcgccgg
     4201 tgcgaagctc accgaggacc tcggtgccca ccctgcacgc gaaagcgttg cccgagtagg
     4261 tgctcccgtg catgtcgaat ttttccgggc ttccgtacgc cttctgccag agctctcgac
     4321 tcgtaacggt ggccgagagc gcgaccatgc ctgcgccgag cgacttgccg agaacgagtg
     4381 cgtcggggag cacccctgcg ctttcgtatg cgaacaggct gccggtgcga ccgaggccgg
     4441 tctgcacctc gtcgagcacg aatagcgtcc cgcggcgctt acacaaggcg cgcgcctcgg
     4501 cgaggtagtt tacaggtggc atgatgaccc cgccctcggc ttggattggc tcgaccacga
     4561 aagcggccgc gcggttctcc gcgagtgagc gcttgagcgc ctctagatct ccgaac
//
LOCUS       DASTKS010001276         8225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_874331, whole genome shotgun sequence.
ACCESSION   DASTKS010001276 DASTKS010000000
VERSION     DASTKS010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874331"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1757)
                     /locus_tag="VFQ35_27270"
     CDS             complement(303..1757)
                     /locus_tag="VFQ35_27270"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9934446.1"
                     /translation="MAGALEPCGCVKDMLGGANHAAAYLDSHRDTQALVLAAGPLLFA
                     EPSLSPERRTQDEWKAEALARSLAGMGLKAWAPGANDFAAGAAKLKQLTDGGPLPLAA
                     NLRVADAALSASAVFQVGAIKVGVAGASLPKHQGKLPEGVNVEEPLAALRAQAAELVR
                     QGTSLRVALLSMPRGEAMRLAELVPDFQVVLVGKGADQGETNDPPSPPSRVGSTLVVE
                     APNHLQSFYVVDLFVKDGRFIFANADQAAEQRNELDSQIKDLADRITSAERAGAQKAD
                     LEARRADLEKLRAKRASLGKSDKPPNESHYRAELVEVRDRLGDDAAVQKRMNEYYRRV
                     NDYNREAFKDKLPPPVAENQSSYVGVEACATCHRSAYEFWQRTGHSRAYPTLQRQFKE
                     YNLDCVGCHVTGYEQPGGSTVTHVDKLTGVQCEVCHGPGSRHVKDITNPAFIVAKPDP
                     ATLCAAKCHHPPHVNPDWNFATALPHIVGKGHGG"
     gene            2224..4263
                     /locus_tag="VFQ35_27275"
     CDS             2224..4263
                     /locus_tag="VFQ35_27275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934447.1"
                     /translation="MTDEQQGAHPDAQRPFGPFVLERRIAVGGSAEVFLARPKVGVVP
                     AARLVVKRLLPNARERGFDLLEREAELHRAVVHPNVVTVFGAGMVGDEPYLAMEYVDG
                     VDLYRLLRRAEAEQRRVPTGLAVFIARRLLAALGAVHSARDPSGQPYGIVHRDVTPSN
                     VYLSVDGEVKLGDFGIARVEQKTKPAAQAAGLKGKFGYLSPEQVAGEPFDHRADLFSA
                     AALFGELLIGERVFPGSGQLAVLLAIRDASIEPLRGAASTLPPGLFAVCEKALARDPD
                     DRFASAEQLSRALEPFERPTEKELRAKLAEWVSWARDSSQFARKLEGQIRESVSRMQA
                     AKSSNSKLRAVRLAETEAPSEVLSRVRRATGQVLEGVTFAKLIEMVATGDLSGDDEVA
                     LMGADFRKIRDVHELARHLLPSTTATTSRVFEPGAPDYSVLLRDTGMLKVLAHLRTRR
                     ESGGLFVQRSLAGGRSSRKEMYLERGRLHHVASSEREELLGEYLVRRGKLTREQLETA
                     LGALQLFGGRLGDTLIGLNLVDAVDVFRAIRDQGRDRVAALCTWEEGLVSFYRGTAPT
                     RVDFPLDLDLASPMMAGCILAAQGEPRTLLPLGSTPILPGLRAEAASSGKERGTAPAS
                     LHLVAGFVGAGLNVDTAIERLVSYHPGGDARIITSKEACAALVTAQALGWIHWAS"
     gene            complement(4347..5114)
                     /locus_tag="VFQ35_27280"
     CDS             complement(4347..5114)
                     /locus_tag="VFQ35_27280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934448.1"
                     /translation="MRLADCYESIGKTASAWRLFVEARSAASISGRADQEKIAAERAM
                     QLEPRLSKIALRIKVSVAGEELKVNGVSIPASAWSAPFPVDPGPQQIEVNAPGYLSWT
                     SDVDVEYGARTVSVDVPRLRPVKDALAVPATELAPAPKKDLRDSLRTIGFVTGTVGLG
                     GVALGGVFAAQAYGSRECRKGDLNGCNEAIGLKNDARTAATRATVAFVAGGALVAAGV
                     TLLVLAPAKRESHAVFQPRLRKVKWLGTAGVTLQGEF"
     gene            complement(5595..7127)
                     /locus_tag="VFQ35_27285"
     CDS             complement(5595..7127)
                     /locus_tag="VFQ35_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934449.1"
                     /translation="MSRGKRLGRFLMQQAGRIRVRLLAVNLVVVLIPVFGLEFARIYE
                     RQLLDGLERDMKNQASLSRAFVEASIDAVAELDTRRIEATLARAARNTRTRIRIVTPD
                     RGVVADSHRDGPPEGKEPAPPLLARDALPSMRWVVTEPEPPTPILSRPEVQRALAGTR
                     ATHTRVAHEPSAVYLFLAEPIRREGKVVAAVYLTRSTNPVLLELHRIRRGLVVVLCVA
                     LALAGVMTLALALSISRPLERLAGAARRIAAGEGRVTLPSGGGGEISELSAAFDEMTK
                     QLESRQRYISQFAADVAHEFKSPLTSIRGAAELLAEGAADDPRARGRFLANIALDAER
                     LDRLVSRLLELSRIEAAEAPRSPVRLRELLERVVERAESPTQRVVLSYVSDVEYTMAR
                     AGDLESAFINLIDNALRASPPSADVHVEVWGNQRDGAVEIRVVDRGHGIPEAIRGKIF
                     ERFFSTDAEGDGTGLGLAIVQRVVEAHRGSVRFDTEVGAGSTFTVCIPLTTVSLREPL
                     AP"
     gene            complement(7132..7827)
                     /locus_tag="VFQ35_27290"
     CDS             complement(7132..7827)
                     /locus_tag="VFQ35_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9934450.1"
                     /translation="MASPKQVLVVDDERRIGEVVEYALSKEGYRVTLAHDGKHGLELF
                     GRETFDLVVLDVMLPDVDGLEICRHIRARGDTPVLFLSARSDEVDKIVGLEIGGDDYL
                     VKPFSPRELVARVRAVLRRSHPVNAKAEAASRLVGAGKLELDPERHEARYAGTRVQLT
                     ATEFALLVALFERPGVVLTRSQLMQRAYSTELTVTERTLDTHVRRIRAKFRGFGADPI
                     STVHGVGYRSGDV"
     gene            complement(7940..>8225)
                     /locus_tag="VFQ35_27295"
     CDS             complement(7940..>8225)
                     /locus_tag="VFQ35_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET9934451.1"
                     /translation="FTGRFGIKESTGGVGLDTLLFQDRFELRQDLFGFGEVVLPRWRV
                     SLGYEFVHRLWLLGGIDDILSGTRRDYFIGAQLKFNDEDLKTILPFAPKP"
BASE COUNT         1344 a         2708 c         2768 g         1405 t
ORIGIN      
        1 ggctttgctc cggagccgca gggctttgct ccggagccgt agggctttgc taccgggccg
       61 tagggctttg ctccggagcc gcagggcttt gctccggagc cgtagggctt tgctccggag
      121 ccgtagggct ttgctccgga gacgtagggc tttgctccgg agccgcaggg ctttgctccg
      181 gggccgtagg gctttgctcc ggagacgcag ggctttgctc cggagacgca gggctttgct
      241 ccggagacgc agggctttgc tgctgggcgg aagggctgtc gggagacgtt gcggggcgag
      301 ggtcagccgc cgtggccttt gccgacgatg tgaggaaggg cggtggcgaa gttccagtcg
      361 gggttgacgt ggggcgggtg gtggcacttg gccgcgcaga gggtcgcggg atcgggcttg
      421 gcgacgatga atgcggggtt ggtgatgtct ttgacgtgcc gggaacctgg gccgtggcag
      481 acctcgcact ggacgcccgt tagtttgtcg acgtgggtga cggtggagcc gccaggttgt
      541 tcatagccgg tgacgtggca gccgacgcag tcgaggttgt attctttgaa ctggcgttgg
      601 agcgtggggt aggcgcgtga gtggccggta cgctgccaga attcgtatgc gctgcggtgg
      661 caggtggcgc acgcttcgac gccgacgtag ctgctttggt tttcggcgac gggcggcggg
      721 agcttgtctt tgaaggcttc gcgattgtag tcgttgacgc gacgatagta ctcgttcatg
      781 cgcttttgca ccgccgcgtc gtcgccgagg cggtctcgca cttcgacgag ttcggcgcgg
      841 tagtggctct cgttcggcgg cttgtcgctc tttccgagcg aagcgcgctt ggcacgcagc
      901 ttttcgaggt cggctcgacg cgcttcgaga tcggcttttt gcgcgccggc gcgctcggcg
      961 gaggtgatgc gatcggcgag atctttgatc tgcgaatcga gctcgttgcg ctgctcggcg
     1021 gcttggtccg cgttggcgaa aatgaagcgc ccgtctttca cgaagaggtc gacgacgtaa
     1081 aacgattgca ggtgattcgg cgcttcgacc acgagagtcg agccaacacg gctcggcggg
     1141 ctcgggggat cattggtttc gccttgatcg gcgcctttgc cgaccaagac gacctgaaaa
     1201 tccggaacga gctcggcgag acgcatcgct tcgccgcgcg gcatgctgag gagcgcgacg
     1261 cgcagcgagg tcccttggcg cacgagctcc gcggcctgcg cgcgcagggc ggcgagcggc
     1321 tcttcgacgt tgacgccctc gggcagcttg ccttgatgct ttggcaagct cgctccggcg
     1381 acacccactt tgatggcgcc gacttgaaag acggcgctcg cggagagcgc ggcatctgct
     1441 acgcggaggt tcgcggcgag aggcaagggc ccgccgtcgg tcaattgttt caacttggca
     1501 gcgccagccg cgaagtcgtt cgcgccaggc gcccacgcct tcaatcccat cccagcgagt
     1561 gagcgcgcga gcgcctccgc tttccattcg tcttgggtgc ggcgttcagg cgagagcgaa
     1621 ggctctgcga agagtaacgg ccccgccgcg agcacgagcg cctgcgtgtc gcggtgcgag
     1681 tcgagatagg cggcagcgtg gttcgcgccg cccaacatgt ctttcacgca gccacagggc
     1741 tccagcgctc cggccacggt ggagatcaaa accagcctta gcgacggcgt agcagcgggc
     1801 ccggccggag gcggcgtagc ccgacagcgg cagccggccc agcctccgaa cagcagtacc
     1861 gccacgccga gtagcctgag tagcagtcgg gaagcccgct gccgccacgc gcggaagtgg
     1921 gatctaggaa tctgttcgcg cccgttgtgg gacagccggc tctgcggcgc gcttgcgcgc
     1981 atgacgcggc tatctagcat ggtttgctga gtttcgcgtt gttgcgcccg agctcaccgc
     2041 gcgaacgatt cggctgccat gccagtcgat gcgccgccgg cctcttcttt gtcgctcgca
     2101 cgttcgagct tcgcgggcct cgcctcggcg gccgcccggt ttccggaagg gtccgctcgc
     2161 ccggtcgcct gcctcgaagc acaaacctcg cttcgccctc gaaagtaacg gcataatgag
     2221 acggtgaccg acgagcagca gggggcgcac cccgacgctc aaagaccttt cgggccgttc
     2281 gtgctcgagc gacgcatcgc cgtgggcggt agcgcggagg tctttttggc acgaccgaag
     2341 gtgggggtcg tccccgctgc gcgcctggtc gtcaagcgct tgctgcccaa cgcgcgcgag
     2401 cgcggcttcg acttgctcga gcgcgaggca gagctgcacc gcgcggtggt gcacccgaac
     2461 gtggtgacgg ttttcggcgc gggcatggtc ggcgatgagc cctacctcgc catggagtac
     2521 gtcgacggcg tcgacctcta tcggctgtta cgccgcgccg aggccgaaca gcgccgggtg
     2581 ccaaccgggc tcgccgtgtt catcgcacgc cgcttgttgg ctgcgctcgg cgcagtccac
     2641 tcggcgcgcg atccctcggg ccaaccttac ggcatcgtcc accgcgacgt gacgccatcg
     2701 aacgtctatc tctcggtcga tggcgaggta aagctcggcg atttcggtat cgcgcgcgta
     2761 gagcaaaaga ccaagcccgc ggcgcaggcg gcgggtctca aaggcaagtt cgggtatctc
     2821 tcacccgagc aagtcgcggg cgagccgttc gatcaccgcg ctgacttgtt ctcggccgcg
     2881 gcgctgttcg gagagctctt gatcggcgag cgggtgttcc ctggcagcgg gcagctcgcc
     2941 gtgctcctcg ccattcgcga cgcgagcatc gaacccttgc gcggcgcggc ctctaccctc
     3001 ccgccggggc tcttcgccgt ttgcgaaaaa gccctcgcgc gtgaccccga cgatcgcttt
     3061 gcgagcgccg aacagttgag tcgtgcgctc gagccgttcg aacgccccac cgaaaaagag
     3121 ctccgcgcca agctcgcgga gtgggtgtcg tgggcgcgag attcgtcgca gtttgcccgc
     3181 aagctcgagg ggcagatccg cgagtccgtg agccgcatgc aggcggccaa gtcctcgaat
     3241 agcaagctgc gcgcggtgcg cctcgcggag acggaggcgc cgagcgaggt gctttcacgg
     3301 gttcggcgcg caaccgggca agtcctcgag ggggtcacgt tcgccaagct catcgaaatg
     3361 gtggctaccg gcgatctgtc gggcgacgac gaggtcgcct tgatgggcgc ggactttcga
     3421 aagatccgcg atgtgcacga gctcgcccgg cacctgttgc cgtcgaccac cgcgaccacg
     3481 agtcgcgtct tcgagccggg cgcgcccgac tactcggtgc tgcttcgcga caccggcatg
     3541 ctcaaggtgc tcgcgcatct tcgcacacgg cgcgaaagcg gcggactctt cgtgcagcgt
     3601 tcactcgcgg gcggacgctc gagccgcaaa gagatgtacc tcgagcgcgg gcggcttcat
     3661 cacgtcgcct cgtcggagcg cgaagagctg ctcggtgagt acctcgtgcg gcgcggcaag
     3721 ctgacgcgag aacagctcga gactgcgctc ggcgcgctgc agctcttcgg cggtcgactg
     3781 ggcgacacgc tcatcgggtt gaacctggtc gacgccgtcg acgtgttccg cgccatccgc
     3841 gatcaaggcc gggatcgggt cgccgctctg tgcacgtggg aagaagggct cgtctccttt
     3901 taccgaggca cggccccgac acgagtcgat ttcccgctcg atttggactt ggctagcccg
     3961 atgatggcgg gttgcatcct cgccgctcaa ggcgagccgc gcacgctgtt gcccctcggc
     4021 tccacgccca ttttaccggg gctccgcgcc gaagccgcga gcagcggcaa agagcgaggt
     4081 acggcgcctg cgtcactcca cctggtggct ggcttcgtgg gcgcgggcct caacgtggat
     4141 actgccatcg agcggctcgt ctcgtatcac ccgggcggcg acgcgcgcat catcaccagc
     4201 aaagaggcgt gcgccgcgct cgtgacggcg caagcgctcg gctggattca ctgggcgagc
     4261 taggcgagcg aaagttctcg ggcgttcggc tcgcgccgta tgacttgtcc agtcgcgggc
     4321 cctcacgagc acagcggacg agcgagctag aactcgccct gcaacgtgac cccggcggtg
     4381 ccgagccact tcaccttacg cagacgcggc tgaaacacgg cgtgcgactc gcgcttggcg
     4441 ggagcgagca cgagcaacgt gaccccggca gcaacgaggg cgccgccagc aacgaaggcg
     4501 acggtcgcgc gggtcgcggc cgtgcgcgcg tcgttcttca acccaatcgc ctcgttgcaa
     4561 ccgttgagat cgcctttgcg gcactctcgg gagccataag cctgagcagc gaagacaccg
     4621 cccagggcca ccccaccgag accgacggtt ccggtcacga acccaatcgt ccttagcgag
     4681 tcacgcaagt ccttcttggg tgcgggcgcg agctcggtcg ccggcaccgc gagagcgtcc
     4741 ttcacggggc ggaggcgcgg aacgtcgacg ctcacggtcc tcgctccgta ttcaacgtcc
     4801 acatcgctcg tccaagacaa gtagcccggt gcattcacct cgatttgctg agggccgggg
     4861 tcgacgggga acggcgcgct ccaagcgctc gccgggatcg aaacgccgtt caccttcagc
     4921 tcttctcctg cgacgctcac cttgatccga agcgcgatct tcgagaggcg tggttcgagc
     4981 tgcatcgctc gctcggcggc gatcttctct tggtcggctc gtcccgatat cgaggccgcg
     5041 gagcgcgctt cgacgaacag cctccaggcg ctcgcggtct taccgatgct ttcgtaacaa
     5101 tcggcgagac gcatcgaggc acctggcgac cgctcgagga ctcgagtgct ctctacgttt
     5161 gcacacgccg cggagcggtc ctctcgaggc gtcgctgctg acgacgacgc ttcgacccgg
     5221 ttgtcctcgg cgcgactgct gttcggaagg agcacgacgc cgcaagcacc gaacagcagc
     5281 gcgcaggcgc cacgtcgaca gaatgaccaa cccgagtgtc tcgccacgat tccagccaca
     5341 cctcggagag ccgcgcctcc cagtggcgtc gacctctccc gagagtgttc gcgatttctt
     5401 acaacggcag catcgaagct tgcttaacga aaatcacgag ctcgtcgcgc tcaccgtcgc
     5461 cgcaaacact ggaaatttcc ggggacgagg cgtaacccac cgtgccaatg cgcgcagcgg
     5521 gatcctgcca caatcacgcc ctgacgcctc tcgcctcgac ctcgttcgag gcagcgcgaa
     5581 cgcgaagcga cttctcacgg cgcgagcggc tcgcgcagac tgactgtggt caggggtatg
     5641 cacaccgtaa aggtactgcc tgcgccgact tcggtgtcga agcggacgct cccgcggtgc
     5701 gcctcgacga cgcgctgaac gatcgcgagc ccgagtccgg tgccgtcccc ttccgcgtcc
     5761 gtactgaaaa agcgctcgaa gatcttgcct cggattgcct cgggaattcc gtgccctcgg
     5821 tcgacgacgc ggatctccac tgctccgtca cgctggttgc cccacacctc gacgtgcacg
     5881 tcagcgctgg gcggtgaagc gcgcagcgcg ttgtcgatca gattgatgaa ggcgctctcg
     5941 aggtcacccg cgcgcgccat ggtgtactct acgtcgctga cataactgag cacgacgcgc
     6001 tgcgtagggc tctccgcacg ctcgacgacg cgctcgagta gctctcgcag gcgcacgggg
     6061 ctacgcggcg cctccgccgc ctcgatccga ctgagctcga gcaatcgtga gaccagccga
     6121 tcgagacgct ctgcgtcgag cgcgatgttg gccaaaaaac gcccgcgcgc gcgggggtcg
     6181 tcggccgcgc cttcggccaa tagctccgcc gcgccgcgga ttgaggtgag cggcgatttg
     6241 aactcgtgcg caacgtcggc cgcgaactgc gagatatagc gttgtcggga ctccagttgc
     6301 ttcgtcattt cgtcgaatgc tgccgagagt tcgctgatct cgccgccacc gccgctcgga
     6361 agtgtgacgc ggccctcgcc agccgcgatg cgtcgggccg cgccggcgag acgctcgagc
     6421 ggtcgggaaa tagaaagcgc gagcgcgagc gtcatcaccc ctgcgagcgc caacgcaacg
     6481 cacagtacga ccacgagccc ccgacgaatg cggtgcagct cgagcaacac ggggttcgtc
     6541 gagcgcgtca ggtacactgc ggccacgacc tttccctcgc gccgaatcgg ctcggcgagg
     6601 aacaggtaga cggcagaggg ctcgtgcgcg acgcgggtgt gcgttgcgcg cgtacccgcg
     6661 agcgcgcgct gcacctcggg tcgcgacaag atcggcgtgg gaggctcggg ctcggtcacg
     6721 acccaacgca tggacgggag cgcgtcccgc gcgagcaacg gcggcgccgg ctccttgccc
     6781 tcgggaggac catcgcggtg cgagtcggcc acgacgccac ggtccggcgt cacgatgcgg
     6841 atccgcgtcc gcgtgtttcg cgccgcgcgg gcgagcgtcg cctcgatgcg tcgcgtgtcg
     6901 agctcggcga ccgcgtcgat gctggcctcg acgaaagcac gcgacagcga ggcctgattt
     6961 ttcatgtcgc gctcgagccc atccagcagc tgacgctcgt aaatacgggc gaattcaagc
     7021 ccgaataccg gaatcagcac gacgaccaaa ttgaccgcga gcagccgcac ccggatccgc
     7081 cccgcctgct gcatgagaaa ccgcccgaga cgcttcccgc gactcatcgc gctacacgtc
     7141 acccgagcgg tagccaacgc cgtgcacggt cgagatgggg tcagcgccga acccccggaa
     7201 cttcgcgcga atgcggcgca cgtgcgtgtc cagcgtgcgc tcggtgaccg taagctctgt
     7261 cgagtacgcg cgctgcatga gttgcgagcg ggtgagaacc acgccggggc gctcgaacaa
     7321 cgctaccagc agcgcgaatt cggtagccgt cagctgcact cgcgtccccg cgtatcgcgc
     7381 ctcgtgacgc tccgggtcga gttcgagttt ccccgccccg accaggcgag acgcggcctc
     7441 cgccttggca ttcaccggat gactgcggcg aaggaccgct cgcacgcgcg cgacgagctc
     7501 acgcggcgag aacggcttca ccaggtagtc gtcgcctccg atttcgagcc ccacgatctt
     7561 gtcgacctcg tcgctgcgcg cggagagaaa gagcaccggc gtgtccccgc gcgcgcggat
     7621 gtgtcgacag atctcgagac cgtcgacgtc gggcagcatc acgtcgagca cgaccaaatc
     7681 gaaggtctcg cggccgaaca actcgaggcc gtgctttccg tcatgcgcca gagttacgcg
     7741 atagccctcc ttcgagagag cgtactcgac gacctcccca atccggcgct cgtcgtcgac
     7801 caccaatacc tgctttgggc tcgccattgc gacgaggttc gagcatagcc gaaacgcgcc
     7861 cggagctcgg cccctcggcg aggcaatttc gaggagagtt gtggcggaaa taaggcgacg
     7921 cggcgcgccg tttaccgcat caaggcttcg gagcgaaggg aaggattgtc ttcagatctt
     7981 cgtcgttgaa cttgagctga gcgccaatga agtagtcgcg ccgcgtcccg ctcaggatgt
     8041 cgtcgatgcc accgagcaac caaaggcggt gcacgaactc gtagccgagc gacacacgcc
     8101 aacgcggcag aaccacctct ccgaagccga acaggtcttg ccggagctcg aagcggtcct
     8161 gaaagaggag cgtgtcgagg ccgacgccgc ccgtcgactc cttgatgccg aacctgccgg
     8221 tgaag
//
LOCUS       DASTKS010001277         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_427211, whole genome shotgun sequence.
ACCESSION   DASTKS010001277 DASTKS010000000
VERSION     DASTKS010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427211"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFQ35_27300"
     CDS             <1..322
                     /locus_tag="VFQ35_27300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934452.1"
                     /translation="ELKRRSLFRNPRGPLWDRSLARGGKEALAELKRSLRLLDARRMV
                     VGHTPTESVPGAEKGHVATRFGGRLVLVDVGLGEGDSAPRAALVVENGRGLEWTPAKT
                     RVLW"
     gene            complement(372..986)
                     /locus_tag="VFQ35_27305"
     CDS             complement(372..986)
                     /locus_tag="VFQ35_27305"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9934453.1"
                     /translation="MAREPVDPQSQPRLAKSNLVALARCHALCLPDTASSRAGAEVLE
                     GLYEALLSDPGARVIWRPSLDEGTPELGAFAAGTVRFRETEALTRSKLRPALFAKLAM
                     RVATMPLHVLSRRRFEGKIPHDGIGYVLTLGSARAIVKGANVPRGSEILSELEEWFEA
                     RGARESWVDTELTNRRAHEFYLRMGYTEVARAFGQVLLKKSLVR"
     gene            complement(926..1684)
                     /locus_tag="VFQ35_27310"
     CDS             complement(926..1684)
                     /locus_tag="VFQ35_27310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934454.1"
                     /translation="MGDQTFSDLTIILPTLNEAGNIESLSSELLEVLPGSRVIVVDDN
                     SSDGTPEVARALAERNPQISLIARQVTPCLTDSIQAGIDAATTDYVAWLDADHSHPPK
                     VLRQLYDVAKVSGCCIATRYSRAEESFMERLPKGEMLAAALSGLLNFSVNHLLRLKIT
                     DYTSGFIVCRRDLLRDHPLQGDYGEYFIELMYYLTRRGVDLREVYYQSPPRQWGESKT
                     GASSWLLMRRGVKYLWLVSRLILNRSPASPRATS"
     gene            complement(1725..>2313)
                     /locus_tag="VFQ35_27315"
     CDS             complement(1725..>2313)
                     /locus_tag="VFQ35_27315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934455.1"
                     /translation="WVHTAGHLEVRAPNDTIQPEMMNPFTVGAAEMALASQVKSFDQL
                     VFFDEAAADRYFLHGYGTPNGFLIVGNPYDRKRWFVKHPQPMVYGVPAGASTVSHTRI
                     TIRDLPAHTLMALRLIPPRFGLDIRTLPETAKFALAWTPNGLNIENVGDQRIEELVIR
                     PKLPISIGWFVLSLFALAGACAALTYPRWRRVWRS"
BASE COUNT          419 a          734 c          767 g          393 t
ORIGIN      
        1 ggagctcaag cgccgtagtc tatttcggaa cccacgaggg ccgctctggg atcgcagcct
       61 ggcgcgcggc ggcaaggaag cgctcgccga attgaagcgc tcgctccgct tgctcgacgc
      121 gcggcgcatg gtcgtcggtc acactccaac cgagtcggtg ccgggcgccg aaaagggcca
      181 cgttgcgacg cgcttcggcg gacgcctcgt gctcgtcgac gtcgggctcg gtgagggaga
      241 cagcgccccg cgggcagcgc tggtcgtcga aaatggccgc ggcctcgagt ggacgcccgc
      301 gaagacgcgc gtactgtggt gaccccgtcg aatcgtcagc cggaaagcgg acgcttcgcg
      361 ctcgcgagta gtcagcgcac gaggctcttc ttcagcagca cttggccgaa cgcgcgcgcg
      421 acttcggtgt agcccatgcg cagatagaac tcgtgcgccc gccggttcgt cagctcggtg
      481 tcgacccagc tctcgcgagc gccgcgcgcc tcgaaccact cttcgagctc ggacaggatt
      541 tcgctgccgc gcggcacgtt cgcgcctttg acgatcgctc gcgccgagcc gagggtaagc
      601 acgtagccga tgccatcgtg cgggattttc ccttcgaagc gccggcgtga gagcacgtgc
      661 aacggcatcg tggcgacgcg catcgcgagc ttcgcgaaca acgccgggcg gagcttgctc
      721 cgcgtcagcg cctcggtctc acggaagcgc accgtaccgg ccgcgaaggc tccgagctcc
      781 ggtgtgccct cgtccaacga tgggcgccaa atcacgcgcg cgccgggatc cgagagtagc
      841 gcttcgtaga gaccttcgag cacctccgcg ccggcgcgtg acgacgccgt gtccggtaga
      901 cacaacgcgt ggcaacgcgc gagcgctacg aggttgctct tggcgaggcg gggctgcgat
      961 tgaggatcaa ccggctcacg agccatagat acttcactcc gcgtcgcatc aagagccagc
     1021 tgctggctcc tgtcttggat tcgccccact ggcgaggcgg cgactggtaa tagacttccc
     1081 tcaaatcgac gccccggcgc gtgaggtaat acatgagctc gatgaaatac tcaccgtagt
     1141 cgccctggag cggatgatct cgcaagaggt cgcgccgaca tacgatgaag ccgctcgtgt
     1201 agtcggtgat cttgaggcga agcaggtggt tcaccgagaa gttcaagagg ccggacagtg
     1261 cggccgcgag catctcgccc ttgggcaagc gctccatgaa agactcttcg gcgcgagagt
     1321 aacgcgtcgc gatgcaacag cccgacacct tcgcgacgtc gtacagctgg cgcagcacct
     1381 tcggcgggtg tgagtgatcg gcgtcgagcc acgccacgta gtcggtggtg gcggcgtcga
     1441 tgccggcctg aatcgaatcg gtgaggcacg gcgtgacctg ccgagcgatc aagcttatct
     1501 gcgggttccg ctccgcgagc gcgcgcgcga cctcgggcgt accgtcgctc gagttgtcgt
     1561 cgacgacgat gacgcgagag ccgggcagga cctccagcag ctcggaagag agcgactcga
     1621 tgtttcccgc ctcgttcagg gtcggcagga ttatcgtgag gtcgctgaaa gtttgatcgc
     1681 ccatcggcac tccttgtgca ttcaaacaga aaaggtaaag cgtctcaaga gcgccacaca
     1741 cggcgccagc gcggatacgt caacgccgcg cacgcccccg cgagcgcaaa gagcgatagg
     1801 acgaaccagc cgatcgaaat cgggagcttg ggacgaatga cgagctcttc gatgcgctgg
     1861 tccccgacgt tctcgatgtt cagcccgttg ggcgtccatg cgagggcgaa cttggccgtc
     1921 tcgggcagcg tccggatgtc caaaccgaac cgcggcggaa tgagcctgag cgccatgagc
     1981 gtgtgcgctg gcaggtcgcg gatcgtgatg cgcgtatggc tcaccgtgct cgctcccgcg
     2041 ggcaccccgt agaccatggg ctgggggtgc ttcacgaacc aacgctttct gtcatacggg
     2101 ttaccgacga tgaggaagcc gttgggggtg ccgtacccat gcaggaagta ccggtcggcc
     2161 gcggcctcat cgaaaaatac gagttggtcg aagcttttca cctgagaggc caacgccatt
     2221 tcggctgctc ccacggtgaa cggattcatc atctcgggct gaatggtgtc gttcggcgct
     2281 cgcacctcga ggtggcccgc cgtatgaacc cac
//
LOCUS       DASTKS010001278        15436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1768562, whole genome shotgun sequence.
ACCESSION   DASTKS010001278 DASTKS010000000
VERSION     DASTKS010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768562"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFQ35_27320"
     CDS             <1..1008
                     /locus_tag="VFQ35_27320"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9934456.1"
                     /translation="SPDVVRSALLAQVDQVMNVGPAHFSEPALELSRRLSGFSGLDKI
                     FFGNSGAEANEAAVKLCRKWGQKRRSGAFEVITTVDGFHGRTLAMTCATGKSGWDEAF
                     PPRVEGFKKVAYGSVDAVASAISERTVAVMVEPIQGEAGAVVPPESYLRELRALCSER
                     GVLLVLDEIQTGLCRTGPLFAFQAAGIRPDMVTLGKGLGGGVPISALLARADVACFDD
                     GDHGSTFGGNALCCAVAIAVLDELTSESGRIRREASSRYLAEALQRIAAAFRARLRGR
                     GHLFGLVLPDERAVALRDACFERGLLVNAARPNVLRFMPSLCVSPEEIDQMSETLLAL
                     R"
     gene            1238..2053
                     /locus_tag="VFQ35_27325"
     CDS             1238..2053
                     /locus_tag="VFQ35_27325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934457.1"
                     /translation="MELRPRLLFVSGLLVNLLIGCGGNTDYQCASAARCPGNDSGGSS
                     GATSASGGEHASGGNTILGGSPGNGGASGLGGAPGKGGAVGLGGMSGARGGSTGLGGA
                     FASGGSVVCCEAYPTCEPGDSDIGPNASCPQGTTCYMRSLCCTTIYCAHPNPTCLALP
                     ACDPGDMEITGECPPDGSCYQRTVCGSTINCLDTACDPSTEPNKKYVATAPSACALID
                     FVCPANTKYFQNGCGCGCEQDPVCPPVIDCQPGGNQDPLCSQASKCPYSMRLD"
     gene            complement(2147..2776)
                     /locus_tag="VFQ35_27330"
     CDS             complement(2147..2776)
                     /locus_tag="VFQ35_27330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934458.1"
                     /translation="MARFSRSSASVAAALAVLSACGGGAFTAGDGQGGNAGGAATGGA
                     VASLGGGGAVSAGGASAGAGTSQGGVGTGGNAQGGRPSNSGGSPAAGTPGAGGFNGFG
                     GSAPGTGGNASGNCDSLWQNYTKEQQAARTCSPDGASKQCSSDFVLPDVCGCEVPVNG
                     TSEHYTKARELYTQWKRSCPAAACLIGCTVNEAPPTCKSTSAGQSMCSW"
     gene            2989..3627
                     /locus_tag="VFQ35_27335"
     CDS             2989..3627
                     /locus_tag="VFQ35_27335"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HET9934459.1"
                     /translation="MRFIATPVNRTMTKQVWAPPERNKLPIFEVLERVLPASGTLLEL
                     ASGTGQHAAYFASRLPTITFLPSDVDAANLSSIAAFVEEAALPNLRAPRFIDVCALAW
                     DAPPVEAIFNANMIHITPWECTEGLFRGAARHLAPGGVLVMYGPYRIHGAHTAESNAV
                     FDAEMQKRDSRFGVRDLEVVAELAQTMGIDFVERVPMPANNQTLVFRRRSVD"
     gene            3816..5039
                     /locus_tag="VFQ35_27340"
     CDS             3816..5039
                     /locus_tag="VFQ35_27340"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9934460.1"
                     /translation="MKGPRTVTGTWVLLSAALALLGCRERSAEKTEPLAFDEKALALG
                     KQALTTYADIAFAAYSDAANGAKELLVAVDAFTGAPSSETLQAARQAWIAARKPYLQT
                     EVFRFYDGPIDSVETLVNTWPIDESYVEAGVAGKAPGILENIDKYPTLTPELLASLNA
                     KEGETSISTGYHVIEFLLWGRDTRADGPGDRPYTDFVTSGTNGELARRRAVYLHIASE
                     LLLRQLEEVRDAWDPARADRYRTKFLSLSAKEGLGLVVKGMGSLSGPELSGERLTVPY
                     ETKDQENEHSCFSDTTSSDVAFDALGIENVGLGRYVRVNGDVVKGTSLIEFIAAHDRN
                     LSERLRAELGSSVAAARAIPSPFDQAIVGTDDAPGRTKIWATIQALQRQTDTLAQVAS
                     AFDLRLSLAAPRTRQ"
     gene            5036..6406
                     /locus_tag="VFQ35_27345"
     CDS             5036..6406
                     /locus_tag="VFQ35_27345"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET9934461.1"
                     /translation="MKRVVFATWVLLAACRSGPRMPHVAESGGDTTVFDATHDAFSLP
                     APGLSPRHRSSFFVGNSFFNQNWVAAPSSVDSRDGLGPLFNARSCSGCHFKDGRGRPP
                     QGEEPLASLLIRVSVGNLRGLHGAPVGDPVYGDQLQGNGVPGVPKEAEVKIDYEEVAG
                     VFPDGERYSLLKPKIRVEELGYGPLAPDVRLSARVAPAMIGLGLLEAVSDETLLALAD
                     ADDRNGDGISGRPNRVPDLATGKLVVGRFGWKAEQPSVLQQIAAAFQGDLGLSTPLLE
                     QQNDTLAQAAARAQPNGGVPEVSAQILREVAVYARVLAVPARREHLDANVAHGEELFG
                     SVGCALCHTPTLRTQQAADLPELHAQDIHPYSDLLLHDLGQGLSDERPVYVASGNEWR
                     TPPLWGIGLVEKVNGHTRFLHDGRARSLQEAILWHGGEATAARARFERLSASDRKSLL
                     AFLRSL"
     gene            6408..7472
                     /locus_tag="VFQ35_27350"
     CDS             6408..7472
                     /locus_tag="VFQ35_27350"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9934462.1"
                     /translation="MQRRSLLRLGALSAFALVGAPGCARPIKREDVLSRLVRDVAVPE
                     LQATLKASERLSSALSALGATPTLTSLSFARAAFAQCLLAWKRAQCFKNGPIVDTNAL
                     VRATFWPPRTAAAEALMNGNDPIDQHRVDSLGVDVKGLYTLEYVLFPAGLGDDAALAL
                     LSSQPGRRRLVFATVLATSVLEYVRRAADAMGDGDAFAKRFAEGGKQSMSRVVGQLIS
                     SVEALAISRLGAVLGLSESRLLKPTDIEGAPSHLSSQIVASELAAVSRIYHGEGRTSL
                     AGLVESLSRPIAARIDSSLSEAERAVAALKAPLEVEVSERRPAVEAALKSVKALERAL
                     KVDLASTLGITLTFQAGDGD"
     gene            7523..8161
                     /locus_tag="VFQ35_27355"
     CDS             7523..8161
                     /locus_tag="VFQ35_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9934463.1"
                     /translation="MSTPFTLYGRQSSHFTRVARMFAVELEVPYEFRPVFDLTSLDPV
                     AYGGNPALKVPSLAGPNGTFYGASNICRELARGAERPLRIVWSESFTTALLANAQELV
                     LHVMNTEVALIMSSMRPSSDAAPSIPDLKLRATLASSLEWLETRWPEILSALPKERDL
                     SFLEVTAFCVVRHLPFRKICEVDRFTALSRFASDFDERPSARATPFQFDVAP"
     gene            complement(8176..9615)
                     /locus_tag="VFQ35_27360"
     CDS             complement(8176..9615)
                     /locus_tag="VFQ35_27360"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9934464.1"
                     /translation="MTTAFSMPGAPTICRVARVQLNAGLEMWARIEPRLSRAREYLRA
                     PVEPASLAAFRALFGVVMGVAALRFLAKGWVRELLVEPSFHFTYPAFEFVRPWSLFGM
                     ELHQLALALLAFAVAVGFHTRLAAAAYFVGFTYVELIDQALYLNHYYLVSLLAALIAA
                     LPFGRFYSVDAWRQPGRRLGEVPRWVLLVVRLQVGVVYFYAGVAKLNQDWLFEAAPLR
                     LWLPARSDLPLVGWLLEYPTTAYVASWVGALFDLSIVFLLSWRRTRAVAFALVVAFHS
                     MTGILFPIGIFPWVMTCAATLFFAPDWPLRLVGRQTVWESPNVRPPGHAAANAPSERY
                     SAPGAFGWLIALHCALQVALPLRQFFERQPSAWTLDGFNFAWNVMLVEKSGHVTFRAR
                     DRLSGRIEEVEPKTFLRRFQEIAMAQDPALVRQAALEVAKQMRARGREVAVYADAIAS
                     LNGRPAQALVDPNVDLTGELPSSWIVPLR"
     gene            9757..11529
                     /locus_tag="VFQ35_27365"
     CDS             9757..11529
                     /locus_tag="VFQ35_27365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934465.1"
                     /translation="MRKINWLFTCLVTGAFGASLGACSSDGAGQSQNEESGQLSLAIT
                     SVPSDVACVRVSVQGSRSSTQAFDVVAGTNATYQLAKLPLGIVTVSAEAFPQACKKIP
                     AGAVATYLTDSPVSVRVDASAVNTVSLKLIRNGRIGVGIDFENGPQSYLLPVADGVTV
                     TPLFTVGDSVNTKPDGMTPYRMVGIPDGLGAFDNGDGSFTLLMNHEIGSGGVARAHGA
                     NGAFVSKWKIRKNDLTVLNGEDLIQNVSRFDVATLSYSAPAQGNVFSRFCSADLAAPG
                     AYYDQASSTGYNGRLFTNGEESGSEGRAWAHDVTGISYELPRLGKASWENVVANPGTG
                     LSTVVAALDDSSPGQVYFYVGTKSNTGNPVERAGLTNGSLFGLAVTGVATEDTALGIP
                     SGTAFTLAALGNVEASTGAALDSASNTAGVTRFNRPEDGAWDPVHPNDFYFATTASFT
                     TPSRLWRVRFSDLTNLAAGGTIEMLLDGTEGQKMLDNITIDKKGHVYLVEDVGNNAHL
                     GKVWRYTIATDTLTQIAQHNPALFTSGAPGFLTQDEEASGIIDASSAIGPGWFLLDVQ
                     AHYATDSELVEGGQLVAIYDPAAL"
     gene            11623..12237
                     /locus_tag="VFQ35_27370"
     CDS             11623..12237
                     /locus_tag="VFQ35_27370"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HET9934466.1"
                     /translation="MRSTTPKDLSGHCPRCLLRLEICMCAAIPRVETRVELLIVQHVT
                     ELRLTSNTGRLAALALPKARIESYGGGEPFDGSVLLAEPAALLYSAAASGPSTQASEL
                     PNITKLVVLDGSFRQARRMYKRIHALRQLPELALPAPSVAPHRLRQPPHEGGMSTLEA
                     IAHALALLEGPEVAAPLHALQAEFVHRMDNLRGRRRDALGRGIN"
     gene            complement(12267..13643)
                     /locus_tag="VFQ35_27375"
     CDS             complement(12267..13643)
                     /locus_tag="VFQ35_27375"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934467.1"
                     /translation="MIGDARAMHWAAAAEAVGIREGQVLVGKYRVERVLGAGGMGLVV
                     AARHLQLDERVAIKLLHAHAQQNPKTLTRFAREARAAAKIKSEHAAWVSDVGTLENGT
                     PYMVMEHLEGDDLASWLRQRGPLPWTQAIDFLLQACEALAVAHSSGIVHRDLKPANLF
                     VTRRLDGTPCVKVLDFGISRVESDVSMTQTAQVLGSPLYMSPEQLAAPESVDARSDIW
                     SLGVILFELVSGSLPFTAATLPDLVAQIMTQPPAALSFARGEGATALDRIVRRCLEKD
                     RALRYPSVAELARALHPFARGAARLSCERIAKILNAPPPSSDHASLLAETIVPPRGSK
                     RALGVGLGLLLVAVVAFAFAWSKRAKVEESPPPPASVVATRAPVAPTARARPPSPAEP
                     VVEPVLSPPINVAQKREPRAPARPLARPTTAEPPSSVEKPPSVDPPREAPSSRQPERK
                     VDPFEDRK"
     gene            complement(13802..15070)
                     /locus_tag="VFQ35_27380"
     CDS             complement(13802..15070)
                     /locus_tag="VFQ35_27380"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9934468.1"
                     /translation="MKPTTTHTFSLLTAVFLFGCSGSEQGTGGGAGGSTASASGGTQS
                     SSGGSQTASGGSQTSSGGSSTSGGTSTASGGATSSGGSSSASGGTASGGGSTTSATGG
                     APSAGSGGAPTDPNTPVALHGPLKVMGTSLVDSKNAPVQLKGPSSMWLNWETKKFAED
                     KQGLAWMRDNWKASVIRAAMGVDTTDTDDYLSAPDTAKAQVNTIVQNAIDNGMYVIID
                     WHDGKADQHQSEAIAFFTEMATKWGSYPNVIYETFNEPTTQSWSGVLKPYHQAVVAAI
                     RAVDPDNVIVLGTRNWSQYVDEPAADPVPGSNLMYTLHFYSCTHTKAIRDRAEAAFGK
                     GLPIFVTEWGATHADGGTPKNPMLCLDEAQLWHDWMNSHKISWAAWKFDACVDQTCYF
                     KDTADPPTNGGWTDDMLNGHATFVRDRMKE"
BASE COUNT         2637 a         5021 c         5063 g         2715 t
ORIGIN      
        1 agccccgacg tggtgcgatc ggcgctgctc gcccaggtcg accaggtgat gaacgtcggg
       61 cccgctcact tcagtgaacc tgctctcgag ttgagccggc gcttgagcgg cttctccggc
      121 ctcgacaaga tcttctttgg caactcgggc gcggaagcga acgaagccgc cgtaaagctt
      181 tgtcgcaagt ggggacagaa aaggcgaagc ggcgcgttcg aggtaatcac taccgtcgac
      241 ggcttccacg ggcgcacgct agcgatgacg tgtgccaccg gaaagtcggg ttgggacgaa
      301 gcgtttcctc cgcgtgtgga aggcttcaag aaggtggcat acggcagcgt agatgccgtc
      361 gcctcggcga tcagcgagcg cacggtggca gtgatggtcg agccgataca gggggaggcg
      421 ggggccgtcg tcccccctga gagctacctg cgggagctgc gcgccttgtg cagcgagcgc
      481 ggtgtgctgc tcgtgctcga cgaaatccag acggggctct gtcgaaccgg gccgctcttt
      541 gcgttccaag ccgctggaat acgtcccgac atggtcacgc tcggcaaagg tctcggcggt
      601 ggtgttccca tttcggcgct cctcgccagg gcagacgttg cctgtttcga tgacggcgat
      661 cacgggagca cgttcggcgg caacgcgctg tgctgtgctg ttgcaatcgc cgttctcgac
      721 gagctcacct ccgagagcgg tcgaatacgc cgcgaggcat cctcgcgcta tttggcagaa
      781 gcgctgcagc gcatcgcggc tgcgtttcgc gcgcggctcc gcgggcgcgg ccatttgttt
      841 gggctcgtgc tccccgacga acgcgctgtg gcgctgcgcg atgcctgctt cgagcgcggg
      901 ttgctggtca acgcggcgcg tccgaacgtg ctgcgcttca tgccctcgct gtgcgtgagc
      961 ccggaagaaa tcgaccagat gagcgagact ctgctggctc tgcgctgagc gcgtagcagg
     1021 gcgttgaaaa atgccctgcc tcacgacaga ggacctcgac gtgggtgggg ctacgcgcat
     1081 gggcgacgct ccacccgctt tgggcgaacg attgtgtacc cgaggagagc gatttacgcg
     1141 gcgcgtccgg actgtgccac ccgtcttggc acaacgtcgc gagtatgagc ccaaatcatc
     1201 atcgatttcg cggtacgcta tttgctcacc gcagaccatg gagctgcgtc cgcgccttct
     1261 gttcgtctcg gggttgttgg tgaacctgct gatcggctgt ggagggaaca ccgactatca
     1321 gtgcgcatcc gcggcacgct gcccggggaa tgattcgggc ggctccagtg gcgctaccag
     1381 cgcgagcggt ggagagcacg cgagcggcgg caatacgatc ctggggggtt cacctggcaa
     1441 cggcggtgcc tctgggcttg gtggggcacc tggaaaaggc ggagcagtgg gccttggagg
     1501 aatgtcgggg gcgcgcggcg gctcgacggg attgggcgga gcgttcgcga gcggtggcag
     1561 cgtagtttgc tgcgaagcgt atcccacctg cgagccaggt gactccgaca tcggcccgaa
     1621 cgcgtcgtgt cctcagggga ccacttgcta catgcggagc ttgtgttgca ccacgatata
     1681 ttgcgcgcac ccgaacccca cttgcctcgc gctccccgcg tgcgatcccg gcgacatgga
     1741 aatcactggc gaatgcccgc cggacggctc gtgttatcag cgcacggttt gcggaagcac
     1801 catcaactgc ctcgatacag cgtgcgatcc cagcaccgaa cccaacaaga agtacgttgc
     1861 cacggctccc tccgcctgcg cgctgatcga cttcgtgtgc ccggccaaca ccaagtactt
     1921 tcagaacggc tgcgggtgcg gttgcgagca ggacccggtc tgccctccgg tcatcgattg
     1981 tcagcctggt ggcaaccaag acccgctctg ttcgcaagcg tccaagtgcc cttactccat
     2041 gcggctcgac taaaaggccc gcgcgaaatt agctgacacc gcggctgccg atcctctcgc
     2101 tgcggcgcgc ggcctcggcg cgctgctctc ggcgaccggc cctcgtctac cagctgcaca
     2161 tgctctgccc tgcactcgtt gatttgcacg tcggcggagc ctcgttcact gtgcagccaa
     2221 tcaggcaggc ggctgccggg cacgaacgct tccattgcgt gtagagctcg cgtgccttgg
     2281 tgtaatgctc gctggtcccg ttgactggca cctcacatcc gcacacatcg gggagcacga
     2341 agtcgctgga gcactgcttg gaggcgccat ccggcgaaca ggtccgagca gcctgctgct
     2401 ctttcgtgta gttctgccag agcgagtcgc agttccccga ggcgttcccg cccgtgcctg
     2461 gcgcgcttcc accgaagccg ttgaagccgc ccgcgccggg agtccccgcg gccgggctgc
     2521 cgccgctgtt cgacggcctc cctccctgcg cgttgcctcc ggtgcccacg ccgccctggc
     2581 tcgttcccgc gccagccgaa gccccgccag cgctcaccgc gcccccacca ccgaggctcg
     2641 cgacagcgcc gcccgtggca gctccgccgg cgttacctcc ctggccatcg ccggccgtga
     2701 acgccccgcc gccgcacgcc gagagcacgg cgagcgccgc cgcaaccgaa gccgatgagc
     2761 gagaaaatct agccatccaa tttctctcga gcaaagccct gctgttggcg gtcctggacg
     2821 aggcacccgc aacaaatgag ggatctctca gttagtaccc cgttgcatcg atttaacgcg
     2881 cgcgaaattt cgaacaagaa tcggggctct cctgctacat agtctcccac tcggcacctc
     2941 cgctcgtgct tcggctccag cggggcgctc tccaaggggg gaatggtcgt gagattcata
     3001 gcaacgcccg tcaacaggac gatgaccaag caagtctggg ccccgcccga gagaaacaag
     3061 ttgccgatct tcgaggtgct cgagcgcgtg ctgcccgcgt ctggcactct gctggagctc
     3121 gcgagcggca ccgggcagca tgcagcgtat ttcgcgtcgc gcctcccgac catcaccttc
     3181 ttgcccagcg acgtcgacgc cgcgaacctg tcgagcatcg ctgcattcgt ggaggaggca
     3241 gccctgccga acctgcgtgc gccgcgcttc atcgacgtgt gcgcgctcgc ctgggacgcg
     3301 cctccggtcg aggcaatctt caacgcgaac atgattcaca tcaccccttg ggaatgtacg
     3361 gaagggttgt tccgcggcgc cgcgcgtcac ctggctccag gcggagtttt ggtgatgtat
     3421 ggcccctatc gtatccacgg ggcgcacacg gccgaaagca acgccgtttt cgacgccgaa
     3481 atgcagaagc gagactcccg cttcggcgtg cgcgacttgg aggtggtggc tgagctcgcg
     3541 caaaccatgg gcatcgattt cgtcgagcgc gtgcccatgc ctgcgaacaa tcaaaccctg
     3601 gtttttcgaa ggcggagcgt ggactgagcc gaccgtaatc acaccgaggc ttgctcggga
     3661 gagctgaaac agaacaaccg cttggcgaca ggtccggtgc aggcccgcgg cggggacccg
     3721 aaagcaagca acggctacgc gtggaaaccg tgacaaatgc gcggcaaacg ccgtggcgac
     3781 cgcgaaggcc agcggatttc gctataccgt cgcgcgtgaa gggtcctcgc acagtcacgg
     3841 gaacatgggt tctactgtcg gcggcgctcg cattactcgg ttgccgcgag cgaagcgccg
     3901 aaaaaacgga gccgctggcc ttcgacgaga aggcgctcgc actcggcaag caagcgctga
     3961 cgacctacgc ggacatcgcc ttcgccgcat actcggatgc agcgaacggg gcgaaggagc
     4021 tcctcgtcgc ggtcgacgcg ttcacgggtg cgccgagctc cgagactctc caggccgccc
     4081 gccaagcctg gattgcggct cgcaagccct acctgcaaac cgaggtcttt cgattctacg
     4141 atgggcccat cgacagcgtc gaaacgctgg tgaacacctg gcctatcgac gagagctatg
     4201 tcgaggcggg cgttgccggc aaggcccccg gaattctcga gaacatcgac aagtatccca
     4261 ctttgacgcc cgagctcctc gcgtcgctga acgcgaaaga gggcgagacc agcatcagca
     4321 ccggttatca cgtcatcgag ttccttctct ggggcaggga cacgcgcgcg gatggcccgg
     4381 gcgatcggcc ctacaccgac ttcgtgacct cgggaacgaa tggggagctc gcgcggcgtc
     4441 gagcggttta cttgcacatc gcgagcgagc tcttgttgcg gcaactcgag gaggtgcgcg
     4501 atgcgtggga cccggctcgt gccgatcgct atcgcacgaa attcttgagc ttgtcggcga
     4561 aagaagggct cggtttggtc gtgaaaggga tgggctcgct gagcggaccg gaactctcgg
     4621 gcgagcgctt gacggtcccc tacgaaacca aagatcaaga aaatgagcat tcgtgcttca
     4681 gcgatacgac gagcagcgac gtggcgttcg acgcgctcgg catcgagaac gtcgggctcg
     4741 gccgctacgt acgcgtgaac ggcgacgtgg tgaagggcac gtcgctcatc gagttcatcg
     4801 ccgcgcacga tcgcaacctc tcggagcgtt tgcgggccga gctcgggagc agtgtcgcgg
     4861 cggcgcgcgc gattccgtcg ccgttcgatc aggcgatcgt cggcaccgac gacgcgcccg
     4921 gacgaacgaa aatttgggct acgattcaag ccttgcagcg ccaaaccgac acgctcgccc
     4981 aagtcgcctc cgcgttcgac ctgcgtctca gcttggccgc tccgcgcacg aggcaatgaa
     5041 gcgagtcgta ttcgccacct gggttctcct cgccgcctgc cgctccgggc cgcgaatgcc
     5101 gcacgtcgcg gagagcggcg gcgataccac cgtgttcgac gcgacccacg atgctttcag
     5161 cctgccggcg cctgggctca gccctcggca tcgctcgtcg ttcttcgtcg gcaactcctt
     5221 cttcaaccaa aactgggtgg cggcgccgtc gtcggtggac tctcgcgacg gtctggggcc
     5281 gctcttcaac gctcggtctt gctctgggtg ccacttcaag gacggtcgcg gtcgcccgcc
     5341 gcaaggtgag gagccgctcg cgagcctctt gatccgcgtg agcgttggaa atttgcgcgg
     5401 gttgcacggc gcgccggtgg gcgatcccgt gtatggagac cagcttcagg gtaacggcgt
     5461 gccgggagtg ccgaaggaag ccgaggtgaa gatcgattac gaagaagtcg cgggggtctt
     5521 tccggatggg gagcgttaca gcttgctcaa gccgaagatc cgcgtcgagg agctcggcta
     5581 cggcccgctc gcgcccgacg ttcgactctc tgcgcgcgtc gctcccgcca tgataggtct
     5641 cgggcttttg gaggcggttt cggacgaaac gctgctcgcg ctcgccgatg cagacgaccg
     5701 caacggtgac ggcatttcgg gtcgcccaaa tcgcgttccc gaccttgcga cgggaaagct
     5761 tgtggtgggt cgattcggct ggaaggcgga gcaaccgagc gtactgcagc agattgccgc
     5821 ggcttttcag ggcgacttgg ggctcagcac gccactgtta gagcagcaga acgacacttt
     5881 ggcgcaagcg gctgcgcgtg cgcaaccgaa tggcggcgtt cccgaggtca gcgcgcaaat
     5941 cttgcgtgag gttgcggtgt acgcgcgcgt cttggcggtg cctgcgcgtc gtgaacacct
     6001 cgatgcaaac gtcgctcacg gagaagagct cttcggttcc gtggggtgtg cgctttgtca
     6061 cacgcccacg ctgcgaacgc agcaagccgc ggatttgccg gagttgcacg cgcaagacat
     6121 ccatccgtat tcggatcttc tgctccacga cctcggtcag ggcctctccg acgagcgccc
     6181 cgtttacgtc gcaagcggca atgaatggcg gacgcctccg ctttggggta tcgggctcgt
     6241 cgaaaaggtg aacggacaca cccgctttct ccacgacgga cgcgctcgca gcttgcaaga
     6301 agccattctg tggcatggcg gtgaggcgac cgcggcgcgc gcgcgtttcg aacgcctatc
     6361 cgcgagtgac cgaaaatcgc tcctcgcgtt cttgcggagc ctctgacgtg cagcgacgga
     6421 gcctgcttcg attgggtgcg ctctccgcgt ttgccctcgt cggcgcgccc ggctgtgctc
     6481 gtcccatcaa gcgcgaagac gtgctctcgc gcttggtgcg ggacgtggcg gttccagagc
     6541 tgcaagcgac gttgaaggcg agtgagcgcc tgagcagcgc actctcggcg ctaggcgcga
     6601 ctccgacgtt gacttctttg agctttgcgc gcgcagcctt cgcgcagtgc ctgctcgcct
     6661 ggaagcgcgc tcagtgcttc aagaacgggc ccatcgtcga caccaacgcg ctcgtgcggg
     6721 cgactttttg gccgccgagg accgctgccg ccgaggcgct catgaacggc aacgacccca
     6781 tcgatcaaca tcgcgtcgat tcgctggggg tggacgtgaa aggcctctat accctcgagt
     6841 acgtgctgtt tccggcaggg ctcggcgatg acgccgcgct cgcgctcttg tcttcgcagc
     6901 ccgggcgacg tcggctcgtg ttcgccacgg tgctcgctac gagcgtactc gagtacgtgc
     6961 ggcgcgcggc cgacgcgatg ggcgacgggg atgcgttcgc caagcgcttt gcggagggcg
     7021 gaaagcagag catgagccgc gtcgtgggcc agctgatctc gagcgtcgag gcgctcgcca
     7081 tcagtcgctt gggtgcggtg ctggggctct ccgagagccg tcttctcaag ccgacggaca
     7141 tcgagggcgc gccgagccac ttgtcgtcgc aaatcgtggc aagcgagctt gcggcggtct
     7201 cgcgcattta ccacggtgag ggtcgaacga gtctcgcggg gctcgtcgag tcgctctctc
     7261 ggccaatcgc ggctcgcatc gactcgagcc tcagcgaggc cgagcgggcg gtcgccgcgc
     7321 tgaaagcccc tctcgaagtc gaggtgagcg agcgccgccc cgcggtggag gccgcactga
     7381 agagtgtgaa ggccctcgag cgcgcgttga aggtcgactt ggcgagtacg ctcggcatca
     7441 ctttgacgtt ccaggctggc gacggcgact aggccgcgct tttgcgctgt tcgctgcgcg
     7501 gctttcgtgc agaatcgcgg gcgtgtcgac gccgttcacg ctgtatggcc gccagagctc
     7561 ccatttcacc cgagtcgcgc gcatgttcgc ggtcgagctc gaggtaccgt acgagttccg
     7621 cccggtattc gatctcacgt cgctcgaccc ggttgcgtac ggcggcaacc ctgcgttgaa
     7681 ggtcccttcg ctcgcaggtc cgaatggcac cttctacggt gcgagcaaca tctgccgaga
     7741 gcttgcacgc ggagccgagc gtccgctgcg tatcgtttgg tccgagtcgt ttacgaccgc
     7801 gttgctcgcc aatgcgcagg agctcgtgtt gcacgtcatg aacaccgagg tcgctctgat
     7861 catgagtagc atgagaccga gctcggacgc ggcgcccagt attccggatc tcaagctgcg
     7921 ggcaactctg gccagttcgc tcgaatggct cgaaacgcgc tggccggaga ttctgtccgc
     7981 cttgcccaag gagcgcgact tgagcttctt ggaggtaacg gccttctgcg tcgtgcgaca
     8041 cctgcccttt cgaaagatct gcgaagtgga tcggttcacg gcgctgtcac gttttgccag
     8101 cgacttcgac gaacgcccct ccgcccgagc gacccccttc caattcgatg tcgctccgta
     8161 gcccggtctg ctcaatcacc gaagcggcac gatccaagag ctcggtagct cgccggtgag
     8221 atcgacgttc gggtcgacga gcgcctgagc ggggcgcccg ttcaaggagg caatcgcgtc
     8281 ggcatagacg gcgacctcgc gcccgcgcgc gcgcatttgc ttggcgactt cgagcgctgc
     8341 ttggcggacc aacgccggat cttgtgccat cgcgatctct tgaaagcggc gcaaaaaggt
     8401 cttgggttcc acctcttcga tcctcccgga gaggcgatct ctcgcccgaa acgtcacgtg
     8461 cccgctcttc tcgaccagca tcacgttcca cgcgaaattg aagccgtcga gggtccaggc
     8521 gctcggctgt cgctcgaaaa attggcgtag aggtaatgcc acttggaggg cacagtggag
     8581 cgcaatgagc cagccgaacg cccccggcgc cgagtagcgc tcgctcggtg cgttcgcggc
     8641 ggcgtgtccc ggcgggcgga cgttcggcga ttcccagacc gtttgtcgcc ctactaggcg
     8701 gagcggccaa tcgggggcga agaatagcgt tgccgcgcag gtcatcaccc aagggaagat
     8761 cccaatcggg aacaagatgc ccgtcatcga atggaaagcg acgaccagcg cgaacgcgac
     8821 ggcgcgcgtt cttcgccagg agagcaagaa gacgatggag agatcgaaga gcgcgccaac
     8881 ccagctcgca acgtaagcgg tggttgggta ctcgagcaac caacccacga ggggtagatc
     8941 cgagcgtgcc gggagccaca agcggagcgg cgcggcctcg aagagccaat cctggttcag
     9001 tttcgcgacc ccggcgtaga agtaaacgac gccgacctgg aggcgcacca cgaggagcac
     9061 ccagcgcggg acttcgccca gccggcgccc tggctgcctc cacgcatcga ccgagtaaaa
     9121 tcgcccgaat ggaagcgccg cgatcagcgc ggcgagaagg ctcaccaagt agtagtggtt
     9181 caggtagagc gcctggtcga tgagctcgac gtaggtgaag cccacgaagt aagcggccgc
     9241 cgcgagtcgc gtatgaaagc cgactgccac cgcgaacgcg agtagcgcga gcgcgagctg
     9301 atgcagctcc atcccgaaga gcgaccatgg cctcacgaac tcgaatgccg ggtaagtgaa
     9361 gtggaagctc ggctcgacga gcaattcgcg cacccaaccc ttggcgagaa agcgcaaggc
     9421 ggcgacgccc atgaccactc cgaagagtgc gcgaaacgcc gcgagcgagg cgggctcgac
     9481 cggagcgcgc aggtactcgc gagctcggct cagtctcggt tcgatgcggg cccacatctc
     9541 gagccccgcg tttagctgca cccgtgcgac gcggcaaatc gtcggcgcgc ccggcatgga
     9601 aaacgccgtc gtcacgtcac ctgcgcgcca cgcaaaaata gcctctccag cgaggactcg
     9661 agcgccgtca ctcaatagcc atggaagctt cggcgactcc tcacgcggcg gcgttcgcca
     9721 cgcggttaaa aggtcgagcc tcaggaggaa ctctctatgc gcaagatcaa ctggctgttc
     9781 acgtgcctcg tcaccggtgc attcggcgcc tcgctcggcg cctgtagctc cgacggagcg
     9841 ggtcagagcc aaaatgaaga gagcggtcag ctgtcgctcg ccatcaccag cgtgcccagc
     9901 gacgtcgcgt gtgtgcgggt gagcgtgcag ggctcgcgct ccagcaccca ggctttcgac
     9961 gtggtcgcgg ggacgaacgc cacgtaccag ctcgcgaagc tgccgctcgg aatcgtgacc
    10021 gtgtccgcgg aagcgtttcc gcaagcttgc aagaagatac ccgccggcgc ggtggcgacc
    10081 tatttgaccg actctcccgt gagcgtccgg gtcgacgcga gcgcggtgaa caccgtgtcg
    10141 ctgaagctca ttcgcaacgg gcgtatcggc gtcggtatcg atttcgagaa cggtccgcag
    10201 tcgtatctgt tgccggtcgc ggacggggtg acggtcaccc cgctcttcac cgtgggcgat
    10261 agcgtcaaca ccaagcccga cggcatgacg ccctatcgca tggtggggat ccccgacgga
    10321 ctcggggctt tcgacaatgg tgacggtagc ttcacgctgc tcatgaatca cgaaatcggc
    10381 agcggtggag tcgcacgcgc gcacggcgcc aacggcgcgt tcgtgtccaa gtggaagatc
    10441 cgcaagaacg atctcaccgt gctaaacggc gaggatctca ttcaaaatgt ctcccgcttc
    10501 gacgtcgcta cgctctcgta ctctgcgccg gcgcagggca atgtcttctc gcgcttttgc
    10561 tcggccgatc tcgcggcgcc cggagcgtat tacgaccaag cctcgagcac cggttacaac
    10621 ggccgactct tcacgaacgg cgaggagtcc ggctcggagg gtagggcttg ggcgcacgac
    10681 gtcacgggga tctcctacga gttgccacgg ctcggaaagg cgtcctggga gaacgtcgtc
    10741 gcaaaccccg ggacgggtct cagcaccgtg gtcgcggcgc tcgatgattc gtcgccaggc
    10801 caggtgtatt tctacgtcgg gacgaagtcg aacacgggca atcccgtcga gcgcgccggg
    10861 ctcacgaacg gctcgctctt cggtctcgcg gtgacaggcg tagcaaccga ggacacggcc
    10921 ttgggcattc cctctggtac ggcgttcacc ctcgcggcgc tcggtaacgt cgaggcttcg
    10981 accggcgcag ccctcgattc tgcgagcaac accgccggtg tcacccgctt caatcgtcca
    11041 gaagacggcg cgtgggatcc ggtgcacccg aacgacttct acttcgccac gaccgcgagc
    11101 tttacgaccc cgagccggct ctggcgcgtg cgcttcagtg atctcacgaa cctcgctgcg
    11161 ggcggaacca tcgagatgct gctcgacggc accgagggcc agaagatgct cgacaacatc
    11221 accatcgaca agaagggcca tgtgtacctg gtcgaggacg tcggcaacaa tgcgcacctc
    11281 ggaaaggtgt ggcgctatac gattgcgacc gacaccctca cgcagatcgc gcagcacaac
    11341 ccggcgctct tcacctcggg cgcgcccggc ttcctgactc aggacgagga ggcttcggga
    11401 atcatcgacg catcgtccgc gatcggtccc ggctggttcc tgctcgacgt gcaggcgcac
    11461 tacgcgaccg acagcgagct cgtcgaaggc gggcagctgg tcgcgatcta cgatccggcc
    11521 gctctctgag gcgctcggcg gcccgggaat tcacggggcg gccaagtgtc gccccgtgcc
    11581 gacctggctt tcactcggga aagcagtagc cttgcgcgcc gcgtgcgctc gactacaccg
    11641 aaggatctat ccgggcactg tcctcgctgc ttgttgcggc tcgagatctg catgtgtgca
    11701 gccatcccgc gtgtcgagac acgcgtcgaa ttgctcatcg tgcaacacgt gaccgagctt
    11761 cgcctcacga gcaataccgg gcggctcgcg gcgcttgcgc tgccgaaggc gcgcatcgaa
    11821 agctatggcg gcggcgagcc gttcgatggc agcgtcttgc tcgcggagcc ggcggcgctg
    11881 ctctatagcg ccgccgcatc ggggccgtcc acgcaggcga gtgagctacc gaacattacg
    11941 aagctcgtcg tgctggacgg aagttttcgt caggcgcgcc ggatgtacaa acgcattcat
    12001 gccctaaggc agctccccga gctcgccttg cccgcgccga gcgtcgctcc gcaccgactg
    12061 cgccagccgc cgcacgaagg tggcatgtcg acgctcgaag ccatcgctca cgcgctcgct
    12121 ttgttggaag ggcctgaagt cgcggccccg ctgcacgcct tgcaagccga gttcgttcac
    12181 cgcatggaca atctgcgtgg acgacggcgc gacgcgctcg gccggggcat caactgacgg
    12241 gcgcgtgacc cgcccggctt cgagcctcac tttcgatctt cgaacgggtc gaccttgcgc
    12301 tccggctgcc ggctcgaagg tgcctctcgc ggcggatcga cgctgggagg cttttcgacg
    12361 ctcgagggcg gttcggcggt cgtcggacga gccagaggac gagcgggggc tctcggctcg
    12421 cgtttttgcg cgacgttgat tggcggcgag agcaccggct cgaccaccgg ctccgcaggg
    12481 ctcggcggtc gcgccctcgc ggtgggcgcc acgggcgctc gcgtcgccac cacgctcgcg
    12541 ggcggcggag gcgactcctc gaccttcgcg cgcttcgacc aggcgaaagc aaacgccacg
    12601 accgcgacga gcagaagccc gaggcccacg ccgagggcgc gcttcgagcc acgcggcggt
    12661 acgatggttt cggcgagaag cgacgcatgg tccgagctcg gcggcggcgc attcaggatc
    12721 ttcgcgatcc gttcgcagga cagtcgcgcg gcgccacgcg caaacggatg cagcgctcga
    12781 gcgagctcag cgaccgaggg gtaacggagc gcgcgatcct tttcgaggca gcgacgtacg
    12841 atgcggtcga gcgcggtcgc gccctcgccg cgggcaaagc tgagcgcggc gggaggttgg
    12901 gtcatgattt gcgcgacgag atcgggcagc gtcgcggccg tgaagggcag ggagccgctg
    12961 acgagttcga acaagatcac accgagcgac cagatgtcac tgcgcgcgtc gacgctttcg
    13021 ggagctgcga gctgctccgg tgacatgtac agcggtgacc cgaggacttg cgcggtctgc
    13081 gtcatcgaga cgtcgctttc gacgcgcgag atcccgaagt cgagcacctt gacgcacggc
    13141 gtcccgtcga gacggcgcgt cacgaagagg ttggcgggct tcaggtcgcg atgaacgatg
    13201 ccgctcgaat gcgccaccgc gagcgcctca cacgcttgca gcagaaagtc gatggcctgc
    13261 gtccacggca aagggccgcg ctggcgcaac cagctcgcca agtcgtcccc ttccaggtgt
    13321 tccatcacca tgtagggagt gccgttctcg agcgtgccga cgtccgacac ccacgccgcg
    13381 tgttcgctct tgatcttcgc cgccgctcgg gcctctcgcg cgaagcgggt cagggtcttg
    13441 gggttctgct gcgcgtgcgc gtgcaaaagc ttgatcgcga cgcgttcgtc gagctggaga
    13501 tgacgagcgg caacgacgag gcccatgccg ccggcaccga gcacgcgctc gacgcgatat
    13561 ttcccgacga gcacctgccc ctcacgaata ccgacggcct ccgccgccgc ggcccagtgc
    13621 atcgcgcgag cgtctccgat cacctcggag cactgttagc acgacgacgc ggcacgggca
    13681 cccaccgcgc gctgcgagca ggtgccgttg ccgccagccc tgtccccaac cgccgcgagc
    13741 cgtcggcggc gattggctcg ccgcgcacgg gtcacgctcg gggcgccggc gctcgccgcg
    13801 ctcactcctt catgcggtcg cgcacgaaag tagcgtggcc attcaacatg tcgtcggtcc
    13861 atccaccgtt ggtcggcggg tccgccgtgt ccttgaagta gcaggtttga tcgacgcacg
    13921 cgtcgaactt ccacgccgcc cagctgatct tgtggctgtt catccaatcg tgccagagct
    13981 gcgcttcgtc caagcagagc atggggttct tcggtgttcc gccgtcggcg tgggtggcgc
    14041 cccactcggt aacgaagatg ggcagccctt tgccgaacgc agcttcggcg cgatcgcgaa
    14101 tcgccttcgt gtgagtgcac gaatagaagt ggagcgtgta catcaggttg ctgccgggga
    14161 cgggatcggc ggcaggctca tcgacgtact gagaccagtt gcgcgtcccg aggacgatga
    14221 cgttgtcggg atcgacggcg cgaatcgcgg cgacgacggc ttggtgatac ggcttcagca
    14281 cgccggacca ggactgcgtc gtcggctcgt tgaaggtctc gtagatgacg ttcgggtaag
    14341 agccccactt cgtggccatt tcggtgaaga aggcgatggc ctcggattgg tgctggtcgg
    14401 ccttgccgtc gtgccagtcg atgatcacgt acatgccgtt atcgatcgcg ttttgcacga
    14461 tggtgttcac ttgcgccttg gccgtgtccg gggcgctcaa gtagtcatcg gtgtcggtgg
    14521 tgtcgacgcc catggcggcg cggatgaccg acgccttcca gttgtctcgc atccacgcga
    14581 ggccttgctt gtcctcggcg aacttcttgg tctcccaatt gagccacatg ctgctcgggc
    14641 ccttgagttg caccggcgcg ttcttcgagt cgacgagcga ggtgcccatg accttgagcg
    14701 gaccgtgcag cgcgacaggc gtgttcggat ccgtcggggc acccccgctg ccggcgctgg
    14761 gcgcgccgcc cgtggcgctc gtcgtgcttc cgccaccgga ggccgtaccg ccgctcgccg
    14821 acgaactgcc cccgctgctc gttgcgccgc cgctggcggt cgatgttccg ccgctggtcg
    14881 agctgccccc gctgctcgtt tgcgagccgc cagatgccgt ctggctcccg ccgctgctcg
    14941 attgcgttcc cccgctcgcg ctcgcggtcg agccgcccgc gccaccgccg gtgccttgct
    15001 cggagccgga gcaaccgaag aggaacactg ctgtcagaag tgaaaaggta tgcgtagtcg
    15061 taggtttcat gtgcgcgctc gaaattccga aatacggaac cggttccata tcacgcgcac
    15121 caacaccggg cgagccgcga actgcagaga aatttcggtg attcaggccg aattggagaa
    15181 ttcttcggtg gcgcaacgac cgaagctcag tgcttcctcc tagcgccgta ttcgaggcag
    15241 aactccgcga gtcactcggc gcgttacgac tgcgtaactc gccctccagg tcacgcggct
    15301 ccaaaggaaa gggggctgct caaccatcga atagccatca ggcgagccgc gccgctcgct
    15361 tgcgacgagt cgatcgcgcc ggcgccttcc ggcaatcccc gagagtgact gcgggctcca
    15421 gtttcgctgt gtggcg
//
LOCUS       DASTKS010001279         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_427494, whole genome shotgun sequence.
ACCESSION   DASTKS010001279 DASTKS010000000
VERSION     DASTKS010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427494"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFQ35_27385"
     CDS             <1..1206
                     /locus_tag="VFQ35_27385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934469.1"
                     /translation="AGEPEPIVPKPKPKPKPKPIVDAGVPDASPDAGPQIQDAGAPDS
                     GPKDASANALADAGATDAGGIAAEGDAGKKSAGDPIADNAAVRSVSDPHPNVRVTVDT
                     EKLRAHRLGARVGALLRGIYQWRDFLGPAGIDPVADIDRIYIVGPQLRRSANVTVIIQ
                     HRMGQERMHEALDRLVQRDPSGGWDEAKVPTAHATADGAPRFFVQPSPNIVVVSPPET
                     LASSQRFPLRRLSRLRGQVIAHMYIATPWRAVRGLPFRVPESIEWASIDVVAADGGGA
                     VIEIEAQDESVVSASKNSDELTRAVLAATTLNLGLLGDILGQRPRSFVQSVEFDSEGS
                     RIVGKVIVTEQQVRDALDMASAWLVPAAPRPSGSGRSSSSSVPRSTPTPTPTANRPAP
                     ATPLERTPH"
     gene            complement(1203..2459)
                     /locus_tag="VFQ35_27390"
     CDS             complement(1203..2459)
                     /locus_tag="VFQ35_27390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934470.1"
                     /translation="MDSASLIEPLSGHPRIGELASLVRASAIDATLARRADYGRRESA
                     APGSALSRGHIPEGLTREDAETPFGNVLAILERGVETESERALLGALMALSIKLAPPT
                     DSDATVQLTAHLVWLAAHTPCDALSALDAALGNEAAPIWEAVAALLTDADRLPSDFGL
                     PEALVAAASLRRSTSDVAREQRSVAAAQVADPALLALLLPRAEELGGELLGEVQAAPR
                     GALLTTVLAVTLILFVMQVVRLVGRFAFGYRRPAAIRLGPQGLELSQRIELMGRVLRD
                     RATIVPLSNLSRVTRDVKYSRVGLYVGLAALTIGTYIGTGFFVDGVRVPGGSWPLLGI
                     AALFLLAGLCIDFVLSTGADALRGRCRVIVVPLRGRTVCVGSLDPQRADSLLSVLAER
                     LRGVPAHTERSESGAETANPEAATSA"
     gene            complement(2591..2995)
                     /gene="panD"
                     /locus_tag="VFQ35_27395"
     CDS             complement(2591..2995)
                     /gene="panD"
                     /locus_tag="VFQ35_27395"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET9934471.1"
                     /translation="MKLNMFKSKIHRATVTHADLSYEGSVTIDSDLLDAADIAPYEAV
                     HIWNVTRGTRLVTYALKGPKGSGAVCINGAAAHLNQPGDLVILATFAEMTREEAHAHV
                     PTVVRVDAKNRIVPHAGPELPGPVLNSLGLSA"
     gene            3167..3424
                     /locus_tag="VFQ35_27400"
     CDS             3167..3424
                     /locus_tag="VFQ35_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934472.1"
                     /translation="MANQSKAELLDTLRTLLREALALRHEGAAHPKLARTSALVDGYM
                     RALLDAGLATQGELLALVSEERTKIAGPATGRASDPAFLAA"
     gene            complement(3514..4224)
                     /locus_tag="VFQ35_27405"
     CDS             complement(3514..4224)
                     /locus_tag="VFQ35_27405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9934473.1"
                     /translation="MVSLGTPTDYDFSLPRIRRELFLQHALPLAHRRGSLLDYGCGNG
                     ANTTLFHADFDDVYGIDVEPDRVQEARARNIQPNVRYELYDGARIPGERMYDAVVSFE
                     VLEHTASDAGSLAEIFGRMKAGGVFMCTVPNKFYLMETHGFRFPYDDVVPYNRIPFLN
                     LLPQAVYDRFGNARIYTRRQIERLVAEAGFTIASSGYLRAPLDKLRNPRLQKLARRVI
                     DRLPEAMGVSVWVVATKS"
     gene            complement(4228..>4684)
                     /locus_tag="VFQ35_27410"
     CDS             complement(4228..>4684)
                     /locus_tag="VFQ35_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9934474.1"
                     /translation="SGERQNLLKVLSRVDVLVINDEEARQLAEEHNIKRAARAILAMG
                     PKRLIIKRGEYGAMLFDGTSIFFAPAYPLENEIDPTGAGDTFAGALLGFLASQSTLDG
                     GTLKRALMTATAVASFCVEGVGTRRTRDLTRNDVAARLEELRTLVRFTE"
BASE COUNT          822 a         1509 c         1607 g          746 t
ORIGIN      
        1 gcaggcgagc cagagccgat cgtaccgaag cctaaaccga agcccaaacc caagccgatc
       61 gtcgacgccg gagtgccgga cgcctcgccc gacgcgggcc cacaaatcca agacgcagga
      121 gcgccggatt caggtcccaa ggacgcgtcc gcgaatgctc tcgccgatgc cggcgcgacc
      181 gatgcaggcg gaatcgcggc cgagggcgac gcgggaaaga agagcgcggg cgatccgatt
      241 gccgacaacg cggcggtgcg ctcggtgtcg gatccgcacc cgaacgtgcg cgtgacggtc
      301 gacaccgaaa agctgcgcgc tcatcgcttg ggtgctcgcg tcggtgcgtt gctgcgcggc
      361 atctaccaat ggcgcgactt cttggggccc gcggggatcg atccggtcgc ggatatcgat
      421 cgcatctaca tcgtcgggcc acagctgcgg cgctcggcga acgtcaccgt catcattcaa
      481 catcgaatgg gacaggaacg catgcacgag gcgctcgatc gcttggtgca gcgcgacccg
      541 agtggcggct gggacgaagc caaagttccg actgcgcacg cgacggcgga cggcgcgccg
      601 cgcttcttcg ttcaaccgtc cccgaacatc gtggtggttt cgccgccgga gacgctcgcg
      661 agctctcagc ggtttccttt gaggcgcctg tcgcggttga ggggtcaggt gatcgcgcac
      721 atgtacatcg cgacgccgtg gcgcgcggtt cgaggcctgc cgtttcgggt gccggagagc
      781 atcgaatggg ccagcatcga cgtcgtcgcg gctgatggcg gcggggccgt catcgagatc
      841 gaagctcaag acgagagcgt cgtcagcgct tcgaagaact cggacgagct cacgcgcgcc
      901 gtgctcgcgg cgacgacgtt gaacctcggg ctgctcgggg acatcttggg gcaacgaccg
      961 cggagcttcg tgcagagcgt ggagttcgac tcggagggaa gccgcatcgt cggcaaggtg
     1021 atcgtgaccg agcaacaggt gcgcgacgcg ctcgatatgg cgagcgcttg gctggtgccc
     1081 gcagcgccgc gaccgagtgg cagcggccgg agctcgtcga gctcggtgcc ccgttcgacg
     1141 cccacgccga cacccacggc gaaccgcccg gctcctgcaa cgccgctcga gcgaacgccg
     1201 cactaagcgg acgttgcggc ttcggggttc gccgtctcgg cgccgctttc gcttcgctcc
     1261 gtatgtgccg gcacgccgcg caagcgctcg gcgagcacgg agagcaagga gtcggctcgt
     1321 tgtggatcga gcgagccaac gcagacggtg cggccgcgca acggtacgac gatgacgcgg
     1381 cagcgcccgc gcagcgcatc agcgccggtc gagagcacga agtcgatgca cagccccgcg
     1441 agcaaaaaca gcgccgcgat cccgagcagt ggccacgaac cacccggcac ccgcacgccg
     1501 tcgacgaaga agccggtgcc gatgtacgtg ccgatggtga gcgcggcgag gccgacgtaa
     1561 aggccgacgc gcgagtactt cacgtcgcgc gtgacgcgag acaaattgga cagcggcacg
     1621 atcgtggcgc ggtcgcgcaa gacgcgtccc atgagctcga tgcgctgaga gagctcgagc
     1681 ccctgcggtc ccaaacgaat cgcggcgggg cgccggtagc cgaacgcaaa gcgcccgacg
     1741 aggcgaacga cttgcatcac gaacaagatc agcgtgactg cgagcaccgt cgtcagcagc
     1801 gctccgcgtg gggcggcctg aacctcgccg agtagctcgc caccgagctc ttcggcgcgc
     1861 ggcaagagca aggcgagcag ggctggatca gccacttgcg cggccgcgac cgagcgctgc
     1921 tcacgcgcga catccgaagt cgagcggcgc aggctcgccg ccgcgacgag cgcctccggt
     1981 agcccgaagt cactcggcag tcgatccgcg tccgtgagga gcgccgctac cgcttcccag
     2041 atcggagcgg cttcgttgcc gagcgccgcg tcgagagcgc tcagcgcgtc gcacggagtg
     2101 tgtgccgcga gccaaaccag gtgagccgtc agctggacgg tcgcgtcaga atcagtgggc
     2161 ggcgccagct tgatcgagag cgccatcagc gctccgagca gggcgcgttc ggattcggtt
     2221 tcgaccccgc gctccaagat cgcgagcacg ttgccgaacg gtgtttcggc gtcctcgcgc
     2281 gtgagcccct cgggaatgtg cccgcggctc aacgccgagc caggcgcggc gctctcgcgg
     2341 cgcccgtagt cggcgcggcg cgccagagtg gcgtcgatgg ctgacgcgcg cacgaggctt
     2401 gcgagctccc cgatacgagg atgcccggac aggggttcga tgagcgaagc gctatccatt
     2461 ccggtggagg ccgtacaaag gcccggcccc tgcttaccac ggaacttccg aaaacctcga
     2521 cgaaacggct acgctttcgt gatgtgcgcg cccgtggcgc gagcgacgcc gtccgccgct
     2581 ccgccgaacg tcacgcagaa agaccgagcg aattcagcac gggcccgggc agctccgggc
     2641 ctgcgtgggg cacgatgcgg ttctttgcgt cgactcggac cactgtgggg acgtgcgcgt
     2701 gcgcttcttc gcgcgtcatc tccgcgaacg tcgccaagat cacgagatcg ccaggctgat
     2761 tcaggtgtgc ggccgcaccg ttgatacaga ctgcgccgct acctttgggg cccttgagcg
     2821 cgtaggtcac gaggcgcgtg ccgcgcgtta cgttccaaat gtggacggct tcgtacgggg
     2881 cgatatcagc ggcgtccaac aaatccgaat cgatggtgac actgccctcg tagctgagat
     2941 ccgcgtgggt gaccgtcgcc cggtggatct tcgacttgaa catgttgagc ttcatgactg
     3001 ctctctgatg cgtcgcgtca tggcggcctg caaagtgctg gtccacctga ctgacttgaa
     3061 gcagagctta ggtcgctagc tgctcaccgt caaacttggc ccgcagtgaa gaggtccggt
     3121 agggctcgaa ttctgcggat cccaggtccg tttggagatg cggctcatgg cgaaccagtc
     3181 caaggctgaa ctgctcgata ctctacgaac cctcctacgt gaagcactcg cgctgcggca
     3241 cgaaggcgcg gcgcacccga agcttgcgcg tacgagtgcc ctcgtcgatg gctacatgcg
     3301 cgctctgctc gatgcgggcc tcgcaaccca aggcgagctg ctcgcgctcg tgagcgaaga
     3361 gcgtacgaag attgcggggc ctgccaccgg tcgcgcctcg gatcctgctt ttttagcggc
     3421 ctgaccgcgc tgtcgcgttc tgcgaagtga cgcggacagc gtgtcggacg gagcgcgagc
     3481 ttcgggcctc aatcgtacgg agcgcggagg cgctcagctc ttcgttgcga cgacccaaac
     3541 cgacacgccc atcgcctccg gaagccggtc gatgacgcgc cgagccagct tttgcagtct
     3601 tgggttccgc agcttgtcga gcggggctcg caggtaaccc gagctcgcga tggtaaagcc
     3661 cgcctcggcg acgagccgct cgatttggcg gcgcgtgtag atacgcgcgt tgccgaagcg
     3721 gtcgtagacg gcctggggta acaggttgag aaacgggatt cggttgtagg gaacgacgtc
     3781 gtcgtaggga aaccgaaagc cgtgagtctc catcaagtag aacttgttgg ggacggtgca
     3841 catgaagacg ccgccagcct tcattcggcc aaagatctcc gcgaggctgc ccgcgtcgct
     3901 cgcggtatgc tcgagtacct cgaacgagac gacggcgtcg tacatgcgct cgccggggat
     3961 gcgcgcgccg tcgtaaagct cgtagcgcac gttcggctga atgtttcgcg ctctcgcttc
     4021 ttgcacgcgg tcgggctcga cgtcgatgcc gtagacgtca tcgaaatctg cgtgaaagag
     4081 ggtggtgttg gcgccgttcc cgcacccgta atcgagcagg ctgccgcgcc gatgtgcgag
     4141 cggcagcgcg tgctgcagga agagctcgcg gcgaatgcgc ggcagcgaga agtcgtagtc
     4201 ggtgggcgtc ccgagcgaaa ccatcggcta ctcggtgaac cgaaccaacg tgcgtagctc
     4261 ttcgagccgg gccgcgacgt cgttgcgggt caaatcacga gtgcggcggg ttcccacgcc
     4321 ctcgacgcag aaggaagcca ccgcggtcgc ggtcatcagc gcgcgcttca aggtgccgcc
     4381 gtcgagggtc gattggctcg ccaagaagcc gagcagcgcc cccgcgaacg tgtcccctgc
     4441 gccggtggga tcgatctcgt tttcgagcgg gtacgcgggc gcgaagaaga tgctggtgcc
     4501 gtcgaacagc atcgcgccgt actcgccgcg cttgatgatg aggcgcttcg gccccatcgc
     4561 caagatcgcg cgggcggcgc gcttgatgtt gtgctcctcg gcgagctgac gcgcttcttc
     4621 gtcgttgatg acgagcacat cgactcgaga cagcaccttc aagagattct gccgctcccc
     4681 ggag
//
LOCUS       DASTKS010001280         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1546767, whole genome shotgun sequence.
ACCESSION   DASTKS010001280 DASTKS010000000
VERSION     DASTKS010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546767"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2567)
                     /locus_tag="VFQ35_27415"
     CDS             complement(<1..>2567)
                     /locus_tag="VFQ35_27415"
                     /inference="COORDINATES: protein motif:HMM:NF023676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/C-terminal domain-containing
                     protein"
                     /protein_id="HET9934475.1"
                     /translation="DDTPLAEKPYAVTEQNFSVRRLQAALNGKHGVFFVHPRESLAVH
                     TERHAEDPRVAHELTLRVDDFGNVTRLAKIAYARQPVAARRPEQSKTLATLTLSSFVN
                     HPGALDPDNTAPAEHWHRLGVGFDASTYELTGLPAPADLEGVLEFDTVDELVENLLSN
                     LGPANDLPFEDTSPATTSLRRRVLDRKQQSFYDSSLSAALPLGQITSLALPYESYTLA
                     LTPGLVAQIVADSLALSGTAFDLALLLSEGGYASREGGYWAPSGRLLFDPTRFYLPVE
                     AVDPFGNHAFVTWDAFSLLVTSTRDSLNNVVSADNDYRVLGPRLVTDANLNRIEASFD
                     ALGMLVRTAVMGKAGETKGDTIQTPTSELQYDLFRWMNEGEPARVHTILREEHSSDNP
                     ASRLQHSYAYSDGFGRVVMQKVQAEPGEVPGMSGVINPRWVGTGRTIFNNKGNPVKQY
                     EPFFSPTSDYESEDVVVQFGVTPILHYDPLGRVIRTDYPDGTFSKVLFDAWREENWDQ
                     NDTVLESRWYQDRGAPSPTGPEPTDPTTRSAWLAAQHAGTPTVTHLDTLGRVFSSIST
                     NRTSGVTAEYQTKTQLDIEGNTLAIFDARQIDLNPLNPLPTITQAFDVLQRRLKLVSA
                     DAGTRLGVADAAGKPLRAWDSRGQTLRSRYDALQRATHAYVRKGALERLLIRTVYGEA
                     LDPTLAQQNNLRGKAYQVYDCAGLVTSGAYDFKGNLLSASRRLAVAYTTEPDWIAAQA
                     LNDPAAILGAVGSLLQTETFTTTSTFDALNRVTRSTAPDGAASATRFVYNEASLLESM
                     HVAVRGAAEAPVVGNIDYNARGQRVRCDYQNGSVTTYQHDPKTFRVSQIRTLRTSDG"
BASE COUNT          407 a          758 c          913 g          489 t
ORIGIN      
        1 ttgccgtcgc tcgttcgcag agtgcgaatc tgactgaccc gaaaggtttt tgggtcgtgc
       61 tgatacgtcg tgacgctacc gttttggtag tcgcagcgga cgcgctgtcc gcgcgcgttg
      121 tagtcgatgt tgccgacgac cggtgcttcc gcggcgccgc gcaccgcgac gtgcatcgat
      181 tcgagcaggc tcgcctcgtt gtatacgaaa cgagtagccg aagctgcgcc gtctggagct
      241 gtcgaccgcg tgacgcggtt gagcgcgtcg aacgtgctgg tcgtcgtgaa cgtctcggtc
      301 tggagcaggc tcccgacggc gcccagaatc gcggcggggt cgttcaacgc ctgcgcggcg
      361 atccagtcgg gctcggttgt gtacgcgacg gcgagcctgc gtgaggcgct gaggaggttg
      421 cccttgaagt cgtacgcgcc gctcgtgacg agcccggcgc agtcgtagac ctgatacgcc
      481 ttgccgcgca ggttgttctg ctgcgcgagc gtcgggtcga gggcttcgcc gtaaacggtg
      541 cggatgagca gccgctcgag cgcgcccttc cgaacgtagg cgtgcgtcgc acgctgaagg
      601 gcgtcgtagc gcgatcgcag cgtttggccg cggctgtccc acgcgcggag cggcttgcct
      661 gctgcgtccg ccacgcccag gcgcgtcccg gcatcggcgc tgacgagctt caaacgccgc
      721 tgcagcacgt cgaacgcttg agtgatggtg gggagcgggt ttagagggtt gagatcgatc
      781 tggcgagcat cgaagatggc gagcgtattg ccctcgatgt cgagctgggt tttggtttga
      841 tattccgcgg tgacaccgct cgtccgattg gtagaaatgg acgagaacac gcgaccgagc
      901 gtatcgagat gcgtcaccgt cggcgtcccg gcgtgctgcg cggcgagcca ggcggagcgc
      961 gtcgtggggt ccgtcggttc gggcccggtt gggctcggtg cgccgcgatc ctgataccag
     1021 cggctctcga gcacagtgtc gttctggtcc cagttctctt cgcgccaggc gtcgaacaag
     1081 accttcgaga aggttccgtc cggatagtcc gtgcgaatga cgcggccgag cgggtcgtag
     1141 tggaggatgg gcgtgacgcc gaattgcacc acgacgtctt cgctctcgta gtcgctggtc
     1201 ggactgaaaa agggctcgta ctgttttacc gggttgcctt tgttgttgaa gatggtgcgc
     1261 cccgttccga cccagcgcgg gttgatgacg cccgacatgc cgggcacctc accgggctcg
     1321 gcttgcactt tctgcatcac gacgcggccg aagccatccg aataggcgta gctgtgctgc
     1381 aggcgcgagg cagggttatc actcgagtgt tcctcccgca ggatggtgtg aacacgcgcg
     1441 ggctcgcctt cgttcatcca gcggaagagg tcgtactgaa gctcgctggt tggagtctgg
     1501 atcgtatctc ctttcgtctc tcccgccttc cccatcacgg cggtccgaac gagcatgccg
     1561 agcgcgtcga agctggcttc gatgcgattg aggttcgcgt cggtgacgag gcgcggcccg
     1621 aggacgcggt aatcgttgtc ggcgctcacc acgttgttga gcgagtcacg cgtgctcgtg
     1681 acgagcagag agaaagcatc ccacgtgacg aaagcgtgat taccgaaagg atccaccgcc
     1741 tcgaccggga gatagaatct cgtcgggtcg aacagcaggc gcccgctcgg tgcccagtaa
     1801 ccgccctcgc gcgaggcata gccaccctcc gagagcagca gcgcgaggtc gaacgccgtc
     1861 ccgctgagcg cgagggaatc ggccacgatt tgcgcgacga gcccaggtgt gagcgcgagg
     1921 gtgtagcttt cgtacggtag cgcgagcgag gtaatttggc cgagtggcag cgcggcgctg
     1981 aggctcgagt cgtagaacga ctgctgcttg cgatcgagca cgcggcggcg caacgaggtc
     2041 gtagcgggcg acgtgtcttc gaaaggcagg tcgttcgcgg gccccagatt cgaaagcagg
     2101 ttctcgacga gctcgtcgac ggtgtcgaat tcgagcacgc cctcgaggtc ggccggagct
     2161 ggtaagccgg tgagctcgta ggtgctggcg tcgaagccga ctccgagccg gtgccagtgc
     2221 tccgcaggag cggtgttgtc cggatcgagc gcgccgggat gattgacgaa cgagctcaac
     2281 gtgagcgtcg cgagcgtttt gctctgctcc gggcgacgcg ccgcgacggg ctgccgggcg
     2341 tacgcgatct tcgcgaggcg cgtcacgttt ccgaagtcgt cgactcgcaa ggtgagctcg
     2401 tgggcgacgc gcggatcttc ggcgtggcgc tcggtgtgga cggcgagtga ctcgcgcggg
     2461 tgaacgaaga agacaccgtg cttgccgttc agtgccgctt gcaggcgacg caccgagaag
     2521 ttctgctccg tcaccgcata gggcttctcg gcgagcggcg tgtcgtc
//
LOCUS       DASTKS010001281        10253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1994270, whole genome shotgun sequence.
ACCESSION   DASTKS010001281 DASTKS010000000
VERSION     DASTKS010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994270"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFQ35_27420"
     CDS             <1..1290
                     /locus_tag="VFQ35_27420"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934476.1"
                     /translation="IGVLSGGTGNDVLIGGLTANTLNGNDGDDVLNGGPAQTVSAQCP
                     TDKLNGGNGNDWFDMGVDDAISQLTKTDCNQTIAGGAGVDVVDYSQRTNNGKVLLKLD
                     GTTLSGEQTTTPKEGDKLGTDLEIVLSGAGDDTITGTDNADFLFGGKGKDAIYGGKGD
                     DYIYGGPGDDSLYGDLGNDTFYELTAYPAATAWNEPLTTLANAPFAGVGLAIFGADVP
                     GGGDDLVFGGGDLTETNKADFTDATNPVKVALCSDAAATNTSMGSCTSAKSDYVGNSL
                     DGTATSGSGTLNTFVNIEYVAGSLTATNTFIGSSNAETFEGGSAVDTILGQGGQDMLF
                     GYPDPATGRSVDNASADILCGGDDDDVLQAGTAATTDGEGQMDQTKSGANQVQLSGAS
                     QQDPAYCTHWANASSPYAVIPGVNLCFGGGTKQSCAN"
     gene            complement(1412..2575)
                     /locus_tag="VFQ35_27425"
     CDS             complement(1412..2575)
                     /locus_tag="VFQ35_27425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934477.1"
                     /translation="MRDLSTGALNPLLALGSCDAVLGEIERVTKIKTPVIRNLWITQH
                     YHSLMHALAVVLGGSNANWSTFATWASKTAGQSIRGEEIPKEMQWLLGEQAKVHERLV
                     EALKLLPGAWAEQFDVFAVPKAVLDNVSRQIAIGNLEVFAELAPLFAKFWDRFSEPSR
                     RVETELDAFVSTLKDGPVDQGGQDWLKTAFTSYFAASRAKTLKEAAEFILYGNLLIGL
                     HEQTRLQPYIAGALDAPFEESSYEALLAAEPDWIERVVRPIFRQVLKLFRKEFEDDWE
                     RLATRYLMTLTLPGGQVLNLSQDVPIGTRPFPQVLNPLEYAELKRLVSTYDPNLDSLK
                     GSGARNWTEIGNRMAFISDLFRSRQQDTSLFEQPFTKEQLAALIEGKVPPGPL"
     gene            complement(2708..4234)
                     /gene="cobA"
                     /locus_tag="VFQ35_27430"
     CDS             complement(2708..4234)
                     /gene="cobA"
                     /locus_tag="VFQ35_27430"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET9934478.1"
                     /translation="MPPAKVWLVGAGPGDPGLLTLRGHEVLQGAEVVLTDALAHPALL
                     EMCPQAEVRYVGKRYGEDSAVQEAITREMIAIAKSGRSVVRLKGGDPLLFARGGEEAL
                     ALAEAGVPFEIVPGISSPVAASAYAGIPLTHRDISKSVTFITGSDRAGKEWSPEAWRK
                     LATATDTICVLMGMRRLEEIVHAILEGGRGAETPAAVIQWGTRPEQRVVTATLAELPE
                     RARSAGLKNPAIIVVGGVVALREQLAWYDKKPLFQKTLFLPRPEAQAAESARAIRERG
                     AEPLVLPMIEIHPPPEPERLERTVAELARYDWVLFTSANGVRRFFDVLRRAQRDARAF
                     GSAKIGVIGPKTASALESFGLRADVVAKEFVGEALANALCEAGAPGKVLLARALVARD
                     ALPQSLTAAGFQVDVVPVYETRGASEEKRARLREGLASGVIDVALFTSSSTVTETLAA
                     LGPDAAAQLSRVVVASIGPVTSRTLTEAGVRVDVSASEYTVSGLLDALERHFSAGETT
                     "
     gene            4387..5244
                     /locus_tag="VFQ35_27435"
     CDS             4387..5244
                     /locus_tag="VFQ35_27435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934479.1"
                     /translation="MRARNQATADRASDEWPDRWFVTNGIVAIGPVSFELLLRSVAER
                     RIPRGSFVRHDSWQVWRRLEEIGALSSDGRQRTIEDLATLSGSLELRAQSPLSEPPPP
                     PSNHELRASIPPNSSGVVSTMRPTRPAAVDPVGVLEHASEFGQALLLTLSTAAAAADA
                     AVGLVHRVRSDLGAVVVVGGHGSTTEHLLGERLAEQDPTLAAARAGHTIVGEPDFGEN
                     SRFMLGRLGRCLHAARGVSMVPLLLYGELVAIFELARSQRPFRAREIARVEDVVEALA
                     ARCVVMGWF"
     gene            5334..6830
                     /locus_tag="VFQ35_27440"
     CDS             5334..6830
                     /locus_tag="VFQ35_27440"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9934480.1"
                     /translation="MDEQRERVVAGVDLGSNSFHMVVARLEADGRIHVLDRLRESVRL
                     ASGLDAHRKLSGKVVERALATLRRFRERLGSLPHDAVWAVGTNTLRQAKDGSEFLEKA
                     EAALGHPIEIISGHEEARLIYLGVAQSIADDVGRHLVVDVGGGSSEFVIGERFETIAL
                     DSLYMGCVGFTDKYFGDGALSRDNFRRAEIAAALELASLREQYRRIGWQRATGSSGTA
                     LAIAEIVRANKWGDAITLDALKQIKTALLEQGNISRIALKGLDPDRVPVFAGGLAIMK
                     ATFESLGIERMHVSSGALREGIVYELLGRVTHEDVKQRTIRTFAERYKVDAAHATRVA
                     DTALALFEQVRTALEFDPEFDPWLLQSAATLHEIGLSIAYAGYHKHSAYILEHADMPG
                     FSRDEQQLLAAVVRAHRRRFSRDLFNRLTKEHKRSGVNLAILLRLAVRLHHSRSAEPL
                     PKPTLLTERGSFRLTFPPGWLDERPLTSADLEDEAAMLQAAGVTLSFR"
     gene            complement(6860..7231)
                     /locus_tag="VFQ35_27445"
     CDS             complement(6860..7231)
                     /locus_tag="VFQ35_27445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934481.1"
                     /translation="MTALSTADVAAIDLIESAATGVLTLAEGLDADELSRSRLTQFEV
                     RRGLLQAASAALALSERALASMPELDVSGWRHTKQRLDTPTGTDAHSVWFAVQALAPA
                     TIGWIRFYRARHPELFSATSP"
     gene            complement(7232..9058)
                     /locus_tag="VFQ35_27450"
     CDS             complement(7232..9058)
                     /locus_tag="VFQ35_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934482.1"
                     /translation="MKLLIDRVHVEELVAKFPRLVALREQLRFGNTAETAFEHLETAE
                     VDFLIALYQRGGPDSQVRAAQLATLQGALNDNGTRFSNDELEEFVPALARFLLRDPQR
                     GWLFGANASVRPLPYVVTRVDFTPANSDETAKVFVELKANAKGSLVTQQIRISSADIA
                     GKTIAEVLAAKGFIAETADLIRAYDDIAARYFDWRSRYGAQFSGRGVGFYAEDPNASH
                     RDTDWSRKDIVVLSTDGTAARLVNDEGVLTQRHTALDTPGDILGQYLRKAAKSNQYDQ
                     EDEVQKSQQMIPPGMFTQIPVHPYILMFHLELHHYLWVHVEDMKLYEYRPDLKQKLVL
                     PEEQTDLIDILTAEMDVLMDDIVAGKSGGTTVLCAGPPGVGKTLTAEVYSEIIRRPLY
                     RVHSGQLGLNVASMEASLKAALMRAQRWGAVMLIDEADVYIRRRNDDISTNAVVGVFL
                     RVLEYYSGLLFLTTNRVDDIDEAIVSRCIALIRYSPPDDEARLRIWEVMLSQFELTVE
                     SKLVHELVQLFPDATGRDIKGLTKLVAKYCRQRDCKPTLPVFKRCAIFRGMEQAPRAE
                     ASAKSDDAKSRDVRPPSRPHGFPPRLDQGVEPSSASTAQEVC"
     gene            9344..9646
                     /locus_tag="VFQ35_27455"
     CDS             9344..9646
                     /locus_tag="VFQ35_27455"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9934483.1"
                     /translation="MPTLLIIPSGKTAEVAPGTRLLTAILDAGEKLTHQCDGKAECGT
                     CHIFVTEGKKSLSKAQRTENERLDATIGVGSKSRLACQAHMGEQNVTVELLGFASG"
     gene            complement(9761..>10253)
                     /locus_tag="VFQ35_27460"
     CDS             complement(9761..>10253)
                     /locus_tag="VFQ35_27460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934484.1"
                     /translation="GFRGELALKFSAKVANEQRPPELVCQQVLTVTRAGDSALPFFAG
                     YLLSFGDLKSLAEVLGKTTNAGGKYFVFCNNIDLGTRYQVPYGGVLWYILPIDEATVY
                     NEMLELLHIDRNTLKKLDTAGKLDAIADAAREYDVTFDVMTYDEGVERMGPVRDPAEN
                     RPV"
BASE COUNT         1835 a         3404 c         3206 g         1808 t
ORIGIN      
        1 atcggcgtcc tcagcggcgg cacgggcaac gacgtgctga tcggtggtct caccgccaac
       61 accttgaacg gtaacgacgg cgacgacgtt ttgaacggtg gccccgctca aaccgtctcc
      121 gcgcagtgcc cgaccgacaa gctgaacggt ggcaacggta acgactggtt cgacatgggc
      181 gtcgacgacg ccatctcgca gctcaccaag acggactgca accagaccat cgcgggcggc
      241 gcgggcgtcg acgtggtcga ctacagccaa cgcacgaaca acggcaaggt cctcttgaag
      301 ctcgacggta cgacgctgag cggcgagcag accacgacgc cgaaggaagg cgacaagctg
      361 ggcaccgacc tcgaaatcgt gctctccggc gcgggcgacg acaccatcac cggcacggac
      421 aacgccgact tcctcttcgg cggcaagggc aaggacgcca tctacggcgg caagggcgac
      481 gactacatct acggtggccc tggcgacgac agtctctacg gcgacctcgg taacgacacc
      541 ttctacgagc tcacggcgta ccccgccgcg accgcgtgga acgagcccct caccaccctc
      601 gccaacgctc cgttcgctgg cgtcggcctc gcgatcttcg gcgctgacgt ccccggcggc
      661 ggcgacgacc tcgtgttcgg cggcggcgac ctgaccgaga ccaacaaggc cgacttcacc
      721 gacgcgacga accccgtgaa ggttgctctg tgcagcgacg cggctgctac gaacaccagc
      781 atgggttctt gcacctcggc gaagtccgac tacgtcggca acagcttgga cggcacggcg
      841 acctcgggct ctggaacgct gaacaccttc gtcaacatcg agtacgtcgc tggtagcttg
      901 accgccacca acacgttcat cggttcgtcg aacgccgaaa cgttcgaagg cggctccgct
      961 gtcgacacca tcctcggcca aggcggccaa gacatgctgt tcggctaccc ggatccggcg
     1021 acgggccgca gcgtcgacaa cgcctctgca gacatcctct gcggtggtga cgacgacgac
     1081 gtcctgcaag ctggcacggc ggccacgacc gacggcgaag gccagatgga ccagacgaag
     1141 agcggcgcga accaggttca gctctccggc gcctcgcaac aagaccccgc gtactgcacg
     1201 cactgggcga acgcctcgag cccctacgcg gtcatccccg gcgtgaacct ctgcttcggt
     1261 ggtggcacca agcaaagctg cgccaactga tagtacctta ccaaaactga gttaacgact
     1321 cgacgcgccg gtggtcttct ctcccaccgg cgcgtttccc tttttgtgga tctcgtgcca
     1381 tcggagacca ccgctcacaa ccgacccccg tttacagcgg ccccggcggt accttgcctt
     1441 cgatcagcgc cgcgagctgt tccttggtga atggctgttc gaacaagctc gtatcttgtt
     1501 ggcgcgagcg aaacaggtcc gagatgaagg ccatgcggtt tccgatctcg gtccagttgc
     1561 gggctccgct tcccttcaac gagtccaggt tcggatcgta cgtcgagacc aggcgcttga
     1621 gctcggcgta ctcgagcggg ttcagcacct gggggaatgg gcgcgttccg atgggaacgt
     1681 cctgactcaa gttcagaact tgacctcccg gcaacgtgag cgtcatgaga tagcgcgtcg
     1741 ccaagcgctc ccagtcgtcc tcgaactctt tgcggaagag cttcagcact tggcgaaaaa
     1801 tgggacgcac gacgcgctcg atccaatccg gctcggccgc gagcagcgct tcgtagctgc
     1861 tctcctcgaa cggcgcgtcg agcgctccgg cgatgtacgg ctgcagtcgc gtctgctcgt
     1921 gcaacccgat gagtaggttt ccgtagagga tgaactcagc cgcctctttc aacgtcttcg
     1981 cgcgactcgc ggcgaagtag ctcgtgaacg ccgtcttcag ccaatcttgg ccaccctggt
     2041 cgacgggtcc gtctttcaac gtggagacga aagcgtcgag ctcggtttcg acacgccggc
     2101 tcggctcgga aaagcgatcc cagaacttgg cgaagagcgg cgcgagctcg gcgaagacct
     2161 ccaggtttcc aatcgcgatc tgtcggctca cgttgtcgag cactgccttc ggcaccgcga
     2221 acacgtcgaa ctgctccgcc cacgcgccgg gcaaaagctt gagcgcctcc accaatcgct
     2281 cgtgcacctt ggcctgttcc ccgagcagcc actgcatttc cttcggaatc tcttcgccgc
     2341 gaatcgattg gcccgccgtc ttcgaagccc acgtcgcgaa ggtcgaccag ttcgcgttgg
     2401 acccgccgag cacgaccgcg agcgcgtgca tcagcgaatg gtagtgttgc gtgatccaca
     2461 gattgcgaat cactggtgtt ttgattttgg tgacgcgctc gatctcgccg agaacggcgt
     2521 cgcaagagcc gagcgctagc aacggattca gagccccagt cgacagatcc ctcatgctgc
     2581 gacgataccg aaggtcgcgt cggcgaggaa cgctcctcgt ctcgctttgc gacgaagcga
     2641 gtgggcgaat gtgcgaagtc ctgcgtccgc ctttacgatg atgcgcgccc gctcgcggcc
     2701 gcaccgttca cgtcgtttcg cccgcgctga agtgccgctc taaagcgtcg agcaggccgc
     2761 tcacggtgta ctcactggcg ctgacgtcga cgcgcacacc tgcttccgtt agcgtacgac
     2821 tcgttacagg gccgatgcta gctacgacca cccgcgagag ctgagccgcg gcatcgggac
     2881 cgagcgccgc caatgtctcc gtcacggtcg aactcgacgt gaacaaggcg acgtcgatca
     2941 cgccgctcgc gagcccctct cggagccgcg cgcgtttttc ttcggaagcg ccgcgcgttt
     3001 cgtacacggg cacgacgtcc acctgaaagc cggcggcggt gagtgattgc ggcaacgcat
     3061 cacgcgcgac gagtgctcgg gcgagcagta ccttacctgg cgctccggct tcgcaaaggg
     3121 cgttcgccag cgcttctccc acgaattcct ttgcgaccac gtccgcacgc agcccgaagc
     3181 tctcgagcgc gctcgccgtc ttcgggccaa tcacgcctat cttcgcgctc ccgaaggcgc
     3241 gcgcgtcgcg ctgagctcgc cgaagcacgt cgaagaaacg tcgcactccg ttcgcgctcg
     3301 tgaacaagac ccaatcgtac cgtgcgagtt cggcgacggt gcgctcgagc cgttctggct
     3361 ccggcggcgg gtgaatctcg atcattggta gaaccagtgg ctcggctcca cgctcgcgaa
     3421 tcgcgcgcgc gctctcggcc gcttgggcct cgggccgcgg cagaaacagc gtcttctgaa
     3481 agagcggctt cttgtcgtac caggcgagct gctcgcgcaa cgcaaccaca ccgccgacca
     3541 cgatgatcgc cgggttcttg aggccagcac tgcgcgcacg ctccggcagc tcggcgagtg
     3601 tcgccgtcac gacgcgctgc tccggccgcg tcccccattg aattacggcg gcgggagtct
     3661 ccgcgccacg tccgccctcc aaaatggcgt gcacgatctc ttcgaggcgc cgcatcccca
     3721 tcaagacgca gatcgtgtcg gtcgcggtcg cgagtttccg ccacgcttcg ggagaccact
     3781 ccttaccagc gcgatcactc cccgtgatga aggtgacgct cttcgagata tcgcgatgag
     3841 tcagtgggat gccggcgtaa gcgctggccg caacgggcga agagatcccg ggaacgattt
     3901 cgaaaggcac tcctgcttcg gcgagcgcca aggcttcttc acccccgcgc gcgaacagaa
     3961 gggggtcgcc gcctttgagc cgcaccaccg agcgcccact cttcgcgatc gcgatcatct
     4021 ctcgggtgat cgcttcttga acggcgctgt cctccccgta acgcttgcct acgtagcgaa
     4081 cctcggcttg agggcacatt tcgaggagcg ctgggtgagc cagcgcatcg gtgaggacga
     4141 cctcggcgcc ctggaggacc tcgtggccgc gcagcgtgag caggcccggg tcacccggac
     4201 ccgcgccaac gagccagact ttggcggggg gcatgccact ccttagtccc acccattggt
     4261 tccgtgaaac ccaaaatcgc gaaccccaac ctgcgggaaa tacgcagcga tttcgatata
     4321 ttcaacaacc actgccgaga atgaggcgtg tgcgtatacg tgtgttatcg tcctacttca
     4381 tcacacatgc gagccaggaa tcaggctact gccgaccgtg cgagtgatga atggcccgac
     4441 cgatggttcg tcaccaacgg gatcgtcgcc atcggacccg tctccttcga gctcttgctg
     4501 cggagcgttg ccgagcgccg catcccgcgc ggctcattcg tacggcacga ctcgtggcag
     4561 gtgtggcgac gcctcgaaga gatcggtgcg ctcagcagcg atggacggca acgcaccatc
     4621 gaggatctgg caacgctgag cggaagcctc gaactccgcg cgcagagccc gctcagcgaa
     4681 ccgccgccgc caccgagcaa tcacgagctc cgcgcgtcca taccgccgaa ctcctcgggc
     4741 gtggtttcga cgatgcgccc gacgcgcccg gcggcggtgg acccagtggg cgtgctcgag
     4801 cacgcaagcg agttcggaca ggcattgctg ctcaccctgt cgaccgctgc agccgctgcc
     4861 gacgcggccg tgggactcgt gcaccgcgtt cgaagcgacc tgggagcggt ggtcgtcgtg
     4921 ggcggacacg gttcgaccac cgagcatcta ctcggcgagc gtctcgctga gcaagacccg
     4981 acgctggcag ccgcgcgcgc tgggcacacc atcgtcggcg agcctgattt cggcgaaaat
     5041 agccgattca tgctggggcg gctgggccgc tgccttcacg ccgcgcgcgg ggtgagcatg
     5101 gttccgttgc tcttgtatgg cgagctcgtg gcgatcttcg agctcgctcg ctcccagcgg
     5161 cctttccgag cgcgagagat cgcgcgcgtc gaggacgtgg tcgaggcgct cgcggctcgt
     5221 tgtgtcgtga tgggctggtt ctaggcgctc gcggctcgtt gtgttgtgat gggctggttc
     5281 caagcgcggg gtcatcgctc gcgctcgccc ggcgattgga gtacgactag cctgtggacg
     5341 agcaacgcga acgcgtggtc gccggagtcg accttggctc caatagtttt cacatggtcg
     5401 tcgcgcgctt ggaggcggac ggtcgcattc acgtgctcga ccgactgcgc gaatcggtgc
     5461 gcctggcgtc tgggctcgat gctcaccgca agctctccgg aaaagtggtc gaacgcgcgc
     5521 tcgctacact gcgccgtttt cgtgagcgcc tcggcagctt gccgcacgat gcggtttggg
     5581 cggttggcac caacaccctg cgccaagcga aggacggctc cgagttcctg gaaaaagccg
     5641 aggccgcgct cggccatccg atcgagatca tttccggaca cgaggaagca cgcctcattt
     5701 accttggcgt cgctcagtcc atcgcggacg acgtcggacg tcacctggtc gtcgacgtgg
     5761 gcggcggcag ctccgagttc gtcatcggcg agcgcttcga aaccatcgcg ctcgacagcc
     5821 tctacatggg ctgcgtgggc ttcaccgaca agtacttcgg cgacggcgcc ctcagccgcg
     5881 acaacttccg gcgcgccgaa atcgcggccg cgctcgagct cgcttcgctg cgcgagcaat
     5941 atcggcgcat cggctggcag cgcgcgaccg gctcttctgg taccgcgctc gcgatcgccg
     6001 agatagttcg cgccaacaag tggggcgacg cgatcacgct cgacgcgctc aagcagatca
     6061 agaccgccct cctcgagcaa ggcaacatct cgcgcatcgc cctgaagggc ctcgaccccg
     6121 atcgcgtccc cgttttcgcc ggcgggctcg cgatcatgaa ggcgaccttc gagtcgctcg
     6181 ggatcgagcg catgcacgtc tcgagcggcg cgttgcgtga aggcatcgtc tacgagctcc
     6241 tcggccgcgt gacgcacgag gacgtcaagc agcggaccat tcgcaccttc gccgagcgct
     6301 acaaggtcga cgccgcgcac gcgacccgcg tcgccgacac ggcgctcgcg ctcttcgagc
     6361 aggtgcggac agcgctggag ttcgatcccg agttcgaccc ctggctcttg cagtcagctg
     6421 cgaccctgca tgaaatcggt ctgagcatcg cgtacgccgg ctatcacaag cacagcgcgt
     6481 acatcctcga gcacgccgac atgcctggct tttctcgcga tgagcagcag ctcctcgccg
     6541 cggtcgtgcg cgcgcaccga cgccgctttt ctcgcgacct gttcaaccga ctgaccaaag
     6601 agcacaagcg ctccggcgta aacctcgcca tcctcctacg cctcgcggtt cgcctccacc
     6661 acagccgcag cgccgaaccc ttgccgaaac ccacgttgct cacggagcgc ggctcttttc
     6721 ggctcacgtt cccacctggt tggctcgacg aacgccccct cacctctgct gacctcgagg
     6781 acgaagcggc gatgctgcaa gccgcgggcg tgacgctgag ctttcgctga gcttcgcagc
     6841 gcgcgctaca gccgagtcgt cagggcgaag tggccgaaaa cagctcgggg tgtcgcgctc
     6901 ggtaaaaccg aatccacccg atggtcgcgg gcgcgagcgc ttggaccgca aaccagacac
     6961 tgtgcgcgtc ggtgccggtc ggcgtgtcga gccgctgctt cgtgtgtcgc catccggata
     7021 cgtcgagctc cggcatgctc gccagggcgc gctcgctgag agccagggcc gcgctcgcgg
     7081 cttgaagtag accgcgacgc acttcgaact gcgtcaaacg ggagcgcgac aactcgtccg
     7141 cgtcgagccc ctccgccagc gtgagcaccc ccgtcgcggc gctctcgatt aggtcgatgg
     7201 cagcgacgtc cgccgtagat agcgcggtca ctcagcacac ctcctgcgcc gtcgaagccg
     7261 agctcggctc gactccttgg tcgaggcgtg gaggaaaccc gtggggccgc gaaggggggc
     7321 gaacgtcgcg cgacttcgca tcgtccgact tggcggaggc ctcggctctc ggtgcctgct
     7381 ccattccgcg gaagatcgcg cagcgcttga acacggggag agtcggcttg caatcgcgct
     7441 gccggcaata cttcgcgacg agcttggtca agcccttgat gtcgcgcccg gtcgcgtcgg
     7501 gaaagagctg cacgagttcg tggacgagct tcgactccac ggtgagctcg aactggctca
     7561 gcatgacctc ccaaatgcga aggcgcgcct cgtcgtcggg agggctgtac ctaatgagcg
     7621 cgatgcagcg ggagacgatc gcctcgtcga tgtcgtcgac gcggttcgtc gtgagaaaca
     7681 agagcccgct atagtactcg agcactcgca agaagacgcc cacgaccgcg ttcgtcgaga
     7741 tgtcgtcgtt gcggcgacgg atgtacacgt cggcttcgtc gatcagcatc accgcgcccc
     7801 aacgctgcgc gcgcatcaag gctgctttca gcgatgcctc catgctcgcc acgttcaagc
     7861 cgagttgacc ggagtggacg cgatagaggg ggcgccggat gatctcggag tagacctcgg
     7921 ccgtgagcgt tttcccgacg cccggcgggc ccgcgcagag cactgtcgtg ccaccggact
     7981 ttcccgcgac gatgtcgtcc atcaagacgt ccatttcggc ggtgaggatg tcgatgagat
     8041 ccgtttgttc ctcgggcaag acgagctttt gcttcaagtc gggccgatac tcgtagagct
     8101 tcatgtcctc gacatgcacc cacaggtaat gatgcagctc gaggtggaac atcagaatgt
     8161 aagggtgaac cgggatttgg gtgaacatgc ccggcgggat catctgctgt gacttctgga
     8221 cctcgtcctc ttggtcgtac tggttgctct tggccgcctt gcgcaaatac tggccgagga
     8281 tgtcgcccgg cgtatcgagc gcggtatggc gctgagtgag gacgccctcg tcgttcacca
     8341 ggcgcgcggc cgtgccgtcg gtcgacagca cgacgatgtc cttgcgtgac cagtccgtat
     8401 cccggtgcga agcgttcggg tcctctgcat agaagcccac accgcgcccg gagaactgag
     8461 cgccatagcg actgcgccag tcgaagtagc gcgcggcgat gtcgtcgtac gcgcgaatga
     8521 ggtcggccgt ctcggcgatg aagcctttcg cggcgagcac ctcggcgatg gtcttgcccg
     8581 ctatgtccgc gctcgaaatg cggatttgct gggtgacgag cgagcccttg gcatttgcct
     8641 tgagctcgac gaagaccttc gcggtctcgt cgctgttggc gggcgtgaaa tcgacgcgcg
     8701 tgacgacgta gggtagcggc cgcacgctcg cgttcgcgcc gaagagccag ccgcgctgcg
     8761 gatcgcgcag cagaaagcgc gcgagcgccg ggacgaattc ctccagttcg tcgttggaaa
     8821 agcgggtacc gttgtcgttc agggcgcctt gcaacgtggc gagctgggct gcgcgcacct
     8881 gcgagtcggg gccgccgcgc tgataaagcg cgatgaggaa gtcgacctcg gctgtctcga
     8941 gatgctcgaa cgccgtctcc gccgtgttgc cgaagcgcaa ctgctcgcgc agcgcaacaa
     9001 ggcgtgggaa cttcgctacc agctcctcta cgtgcacgcg gtcgatcaag agcttcatgc
     9061 tgcctcagcc gtttggcgac atgcgaatgc atgccggcaa cgaccgaagc aagatcggta
     9121 cctccctgcg agtcgctccc gactccgagg cgcccggtag cttcgctcgt tcgccttttg
     9181 tagagaaccc gacaccattc gtgtccgaga tcgtccgaaa taggtcaaac ccggcacctc
     9241 cagccgccag cgcgcgcggc ttcttcggcg cggcgtcgcg cctccgaagg cgttggcgtg
     9301 aatcttgata gccgccgcgc gaccgagcga ttggagacga gacatgccga cgttgttgat
     9361 catcccgagt ggaaaaaccg ctgaagtggc tcctggaacc cgcctattga ccgccatcct
     9421 cgacgcgggt gagaagctaa ctcaccaatg cgacggcaaa gccgagtgcg ggacgtgcca
     9481 catcttcgtt accgaaggta agaagagcct ctccaaagcg cagcgtaccg agaacgaacg
     9541 actggacgcg acgatcggtg tcggcagcaa gtcgcgcctc gcctgccaag ctcacatggg
     9601 cgaacagaac gtcacggtgg aactactggg cttcgcctcc ggctgaatcg acgagcttct
     9661 gtagatagcg acggaacgat gggctcggta ccgttcgccc cctgccgtgg gcggtgggcg
     9721 aaattggctg aaccgagccc tctattccgc gagcagacgc tcagacgggg cgattctctg
     9781 ctggatcgcg aacgggaccc atgcgctcca caccctcgtc gtaggtcatg acgtcgaagg
     9841 tgacgtcgta ctcgcgcgcc gcgtccgcga ttgcgtcgag cttgcccgcc gtgtcgagct
     9901 tcttcagcgt gttgcgatcg atgtgcaaga gctcgagcat ctcgttataa acggtcgctt
     9961 cgtcgatcgg gagtatgtac cagagcacac cgccgtaggg gacctggtag cgcgtgccga
    10021 gatcgatgtt gttgcaaaac acgaagtact tgccaccggc gttcgtggtc ttgccgagca
    10081 cctccgcgag tgacttcagg tcaccgaacg aaagcaaata tccggcaaaa aacgggaggg
    10141 cagagtcgcc cgcgcgcgtg accgtcaaca cctgctggca aacgagctcg ggcggacgct
    10201 gctcgttcgc gaccttggcg ctgaatttca acgccagctc gcctcgaaaa ccg
//
LOCUS       DASTKS010001282         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_206301, whole genome shotgun sequence.
ACCESSION   DASTKS010001282 DASTKS010000000
VERSION     DASTKS010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206301"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFQ35_27465"
     CDS             <1..633
                     /locus_tag="VFQ35_27465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9934485.1"
                     /translation="ETANERQARLAAEQRADAAVQSLRQIASVQEEQRGLVITLEGAV
                     LFTTGKSELLPLAQQKLDQVADALKQSDSPKIVVEGYTDSRGADDANLRLSQQRAESV
                     RTYLVSRGVKSESISAVGKGEANPIASNETPEGRANNRRVEIIVQPKSGQGPSSSGSS
                     SSGMGSGNSGAMPNGSTPGVSSGSTGTGSAPMGTGSGTGNANGRNSNNPR"
     gene            complement(870..2087)
                     /locus_tag="VFQ35_27470"
     CDS             complement(870..2087)
                     /locus_tag="VFQ35_27470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934486.1"
                     /translation="MSSRLPASRVPSSQCEKTLPAASSRDSGQHRKRIGQYRLHGVLG
                     AGGMAKVYLAEAGRGSVRRLYAVKVPHDEFANAPDFVRMFENESTLAAELQHPHVCSA
                     IDYGHHDGTPYLAMEFLKGRSLAALRPVSDPASDPMLHAARCARVIADACEGLQAIHE
                     YGAEQFPPPRIVHRDISPDNLLLTTDGFVKILDFGLAKVDTRREKTEPGLLKGKMAYI
                     APELLAGEEPSPRSDVWSLGVVAWEMLTGRRLFAEPTDVSTLVAVREQPIQSPSTVVP
                     GIPKSLDAVILRALTRDPAERFQSAEEFGRELWAFMASERRVVRHPELAAWLHELFPA
                     DRDAHQSISSWDWTDDEFSSPEAIEELESARTPIASRTPTVESSPQSPPKSGPRRSSA
                     RDAWRWLKSAVGL"
BASE COUNT          482 a          767 c          790 g          392 t
ORIGIN      
        1 gagaccgcga acgagcgcca agcgcgcctc gccgcagagc agcgcgccga cgcggcggtg
       61 cagagcctgc gccaaatcgc cagcgtccag gaagagcagc gcggcctcgt gatcacgctc
      121 gaaggcgccg tgctctttac caccggcaag agcgagctcc tgcccctcgc gcagcagaag
      181 ctcgaccaag tcgcggacgc tttgaagcag agcgactctc ccaagatcgt ggtcgagggc
      241 tataccgact cgcgcggcgc ggacgacgcc aacctgcgcc tttcgcagca gcgcgccgag
      301 tcggtgcgca cctacctcgt gagtcgcgga gtcaaatcgg agagcatctc cgcggtcggc
      361 aagggcgaag ccaacccaat tgcttcgaac gagacgcccg aggggcgcgc caataaccgg
      421 cgcgtcgaaa tcatcgtaca gcccaagagc ggtcagggcc cttcgagctc cggttcttcg
      481 agctcgggaa tgggctccgg caattcgggc gcgatgccga acggttctac gcctggcgtg
      541 agctccggtt cgactggaac cgggtcggcg cccatgggta ccggaagcgg taccggcaac
      601 gccaacgggc gcaactcgaa caatccgcgc tgactctgga tcggtcaacg cggcgaacgc
      661 cccgcgtcga gccctcggca agctcgacgc ggggcgatca cttatttaac gctggcgggc
      721 gccatcccaa ccccgaacaa tcgcgtcgcg aggggaggga caagcgtcac gtgagccgaa
      781 agcaaagagc tgttcgattg atagcgaggc agcttccgtg ggttgagcgc ccccacgagt
      841 catctacgcg agcgcctcgc aatatcgggt cagagcccga ccgcgctctt caaccagcgc
      901 caagcgtcgc gcgcggagga acggcgcgga ccactcttgg gtggagattg tggagaggac
      961 tcgacggtcg gcgtgcggga cgcgatgggc gtgcgtgcgg actcgagctc ctcgatggcc
     1021 tccggcgaag agaattcgtc gtcggtccaa tcccaggagc tgatggattg gtgcgcgtct
     1081 cgatccgcag ggaagagctc gtgtagccac gcggcgagct cggggtgtcg caccacgcgc
     1141 cgttcgctgg ccatgaaggc ccaaagctca cgcccgaatt cctccgcgct ttgaaatcgc
     1201 tcggccgggt ctcgagtcaa cgccctcaga atgacggcgt cgaggctctt tggtatcccg
     1261 ggaacgacgg tcgatggcga ttgaattggc tgctctcgca ctgccacgag cgtcgagacg
     1321 tcggtgggct cggcgaagag tcgtcgcccg gtgagcattt cccacgcgac gactccgagc
     1381 gaccagacgt ccgagcgcgg gctcggctcc tcgcccgcga gaagctccgg cgcgatgtac
     1441 gccatcttcc ccttgagcaa ccccggctcg gttttttctc ttcgtgtgtc gaccttggcc
     1501 agcccaaagt cgaggatttt cacgaagccg tcggtcgtga gaagcaggtt gtcggggcta
     1561 atgtcgcgat ggacgatgcg cggtggagga aactgctccg ccccgtactc gtgaatggct
     1621 tgcagcccct cgcaagcgtc ggcgatgacc cgagcgcagc gtgctgcgtg gagcatcggg
     1681 tccgacgcgg gatccgaaac cgggcgcagc gcggcgagtg agcgcccctt caaaaactcc
     1741 atcgccagat acggcgtgcc gtcatggtgc ccgtagtcga tggccgagca gacgtgcgga
     1801 tgctgtaact ctgccgccaa cgtcgactcg ttttcgaaca tgcggacgaa gtcgggcgcg
     1861 ttcgcgaact cgtcgtgagg cactttgacc gcgtagagtc gacgcacgct tccgcgaccc
     1921 gcctcggcca agtagacttt cgccatgccg cctgccccga gaacgccgtg caagcgatac
     1981 tgcccgatgc gttttcgatg ctgcccgcta tcgcgcgacg aagccgccgg aagcgtcttc
     2041 tcgcattgac tcgaaggaac tcgagaagcg ggtaagcgac tgctcacgcg agcaccgcaa
     2101 gcgagatccg tgccgatgct tcgatgcctc gctcggggca ccgaatttcg ctatctttcc
     2161 aagtgcgccg cgaaagggtc gataaatgac caacaggggg atgccacggg gcaaagcgga
     2221 gctatacaaa aacgccacag tgtggcgatc atgaatggcc ggtgccgtag aaaaggcctg
     2281 aaaaagcgca aaatagccct ggcacgctag gtgcacaagg ttgacccaat tgcaggaccg
     2341 ccacaggcaa ccgaggaatg ggcatgaagg aacgagtact gatagtcgac gatgaccaga
     2401 gccagtgcga cctgttcgcg atggctctcg a
//
LOCUS       DASTKS010001283         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1769193, whole genome shotgun sequence.
ACCESSION   DASTKS010001283 DASTKS010000000
VERSION     DASTKS010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769193"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..693)
                     /locus_tag="VFQ35_27475"
     CDS             complement(43..693)
                     /locus_tag="VFQ35_27475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934487.1"
                     /translation="MRLPRAVAAFSCVLVAVCACGESTGSSQSQGGRSGFGSSGGSSA
                     LEGGNGPTSGGVAGSVECLSAAGAANVFVADISGTGCLPRSLPVTEGRARCAVVEVFP
                     SVCRCAGAPGERTPSPAVDLAVRSHLQAAGAPFACDAVCVCALDQLDGAALASCQNDE
                     QPNAPPGFCYVDPSQGVGSAELVRDCTTSDARRLRYVGERFPGNGSAWMIACGDGI"
     gene            complement(715..882)
                     /gene="rpmG"
                     /locus_tag="VFQ35_27480"
     CDS             complement(715..882)
                     /gene="rpmG"
                     /locus_tag="VFQ35_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9934488.1"
                     /translation="MRENIRLVSSAGTGFTYYTTKNKRTQTAKLELKKYDPVARKHVP
                     FKEAKMPPHSK"
     gene            1070..1219
                     /gene="rpmH"
                     /locus_tag="VFQ35_27485"
     CDS             1070..1219
                     /gene="rpmH"
                     /locus_tag="VFQ35_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9934489.1"
                     /translation="MKRTYQPHNTSRLRTHGFRQRMSTKGGRKVLSNRRRKGRWRLTV
                     STYQK"
     gene            1229..1669
                     /gene="rnpA"
                     /locus_tag="VFQ35_27490"
     CDS             1229..1669
                     /gene="rnpA"
                     /locus_tag="VFQ35_27490"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9934490.1"
                     /translation="MAPPVTPGGGHVREPRGTFAKRERVRKRREYLEIQAQGRRVNSP
                     HFVLVVRAREPSRDGVVRLGITASRKVGVAVVRSRIRRLIREAFRATRELFPRDADVV
                     VIVKRAPQELRLSDVVLEFQSVQRNLSRRIDEARQALNNKRSEP"
     gene            1666..1926
                     /gene="yidD"
                     /locus_tag="VFQ35_27495"
     CDS             1666..1926
                     /gene="yidD"
                     /locus_tag="VFQ35_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9934491.1"
                     /translation="MSRGIEEKPSWLVQLLLVAIRAYQLLIAPLLGPVCRFHPSCSRY
                     TAECLRLHGAARGSYLGVRRILRCHPWNPGGYDPPPPLRAQR"
     gene            2022..3860
                     /gene="yidC"
                     /locus_tag="VFQ35_27500"
     CDS             2022..3860
                     /gene="yidC"
                     /locus_tag="VFQ35_27500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9934492.1"
                     /translation="MDRSNLGRLLLMLAAVFLFMQFGPKLFGGGSSAHQPLLPENERA
                     PATRAPEKICDIWGPRFHAQLSTRGATVKHFQLTTAKYQKNGKPIDLSTTPDVEERRQ
                     LRFHFTNPAAKIPADKAQLAFDKLDFELAAADGKSCEFVYRDARTELREVVKLTGRPY
                     ELVAEAKLTNLSSTPLKHALTIDSDTWRTDHEVKATMFRVSPLLTHVECVPNAGNATR
                     LTAQDFEPSDFEKAEHFPRNELSGGDFFQVPGKPEVAAVSNAYFSHALVPLAGPAPTC
                     QLQIEERWDSRKFASKAKDDNAGAFYRARFAYPSRELAPNESTQYSVLTYIGPKERSV
                     LADAGGGQHRLIELIDLGFFSAIAKILVAFLLKVHSVIPNWGVAIIVLTITARSLLFP
                     LALPGIKGMIKMRELKPEIDALNERFKDDPQQKGLAQMELWRKHNVNPLKGCLPQLAS
                     MPVWFALYTTLQTAVELYNIPFLWFPDLSAADPLYILPFIIGTTSFFQQKLMPMQGDP
                     AQQKMMLYFMPAMFTVFMLFLPAGLGVYMFTNGLLGILQQQAVERHVRRSVRPGGGSG
                     SGDIKVKVIEDSGSNASNKKEKGAAKAGRSSSGSDDDSRLLGEGKA"
     gene            3862..>4068
                     /locus_tag="VFQ35_27505"
     CDS             3862..>4068
                     /locus_tag="VFQ35_27505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934493.1"
                     /translation="MSDTQDFSDEGWDNADPDAPGSDHEHDAPSASDNGEGSASAEGA
                     ETNGHDSAGRRRRSRGRGRGREHDD"
BASE COUNT          771 a         1372 c         1251 g          674 t
ORIGIN      
        1 gcgccgcgta gcttcgagcg actcggctgc gcgcgacaga gcctagatgc cgtcaccaca
       61 agcgatcatc cacgcgctgc cgttgcccgg gaagcgttcc ccgacataac gcaatcgacg
      121 cgcgtccgac gtcgtgcagt cgcgcacgag ctccgcggag ccaaccccct gtgaggggtc
      181 gacgtagcaa aagccgggcg gcgcgttggg ctgctcgtcg ttttgacaac tcgcgagggc
      241 ggcgccatcg agttggtcca aagcacacac gcacaccgca tcgcacgcga acggcgctcc
      301 agccgcttgc aggtggctcc gcaccgcaag atcgacagcg ggcgacggcg tccgctcgcc
      361 gggggcacca gcgcagcggc acacggacgg gaaaacctcc accaccgcgc agcgggctcg
      421 tccctcggtc acgggtaagc tgcgcgggag gcagcctgtc cccgaaatat cggcgacgaa
      481 cacgtttgcc gcgcccgcgg cgctcagaca ctcgaccgag cccgccacgc cgccagacgt
      541 cggaccgttg ccgccctcta gagcactcga accaccactg ctcccgaagc cgctgcgacc
      601 gccctgcgac tgcgacgagc cagtcgactc gccgcaagcg caaacggcca cgagcacgca
      661 cgagaacgcc gcaaccgcgc gcggcagtct caagcccgcc ccgagatcac tcgcttactt
      721 cgagtgagga ggcatcttcg cctccttgaa ggggacgtgc ttgcgggcga cgggatcgta
      781 cttcttcagc tcgagcttcg ccgtctgggt acgcttgttc ttggtcgtgt agtaagtgaa
      841 gccggttccg gccgaggaaa cgaggcgaat gttctcgcgc atagcgagcg ggcgtttaac
      901 atgcccgtcc cactctcgca accagctcaa actcgtcatc ggaccgcaaa aacacgtcgg
      961 ccgagcgacc aagaggagcg gttcgtggaa aaatcgggag tcgagccttg acttctgatc
     1021 cgtatcgtgg cgaattcccc gccccccgac ctcttcggac ggtgtctcaa tgaagcgcac
     1081 gtatcagccc cacaacacca gccgcctccg cacgcacggc ttccgccagc gcatgagcac
     1141 caagggcggt cgcaaagtac tctccaaccg gcgccgcaaa ggccgctggc ggctcaccgt
     1201 ttccacgtac cagaagtaag gtcgggacgt ggcgccgccg gtaacgcctg gcggcggtca
     1261 tgtgagagag ccgcgcggaa ccttcgcgaa gcgcgagcgc gtccgaaaac ggcgcgaata
     1321 cctggagatc caggctcagg gtcggcgtgt gaactcgccg cacttcgtgc tggtggtgcg
     1381 agcgcgggag ccgagccgtg atggcgtcgt tcgcctgggc attaccgcct cacgcaaggt
     1441 gggagtcgcg gtggttcgct cgcgcatccg ccgcctcatt cgtgaggcct tccgggctac
     1501 acgtgagctt ttcccccgcg acgccgacgt cgtcgtcatc gtgaagcgcg ctccccagga
     1561 gctccggcta tccgacgtcg tcctcgagtt ccagagcgtg cagcggaacc tttcgcggcg
     1621 catcgacgag gcgcgccagg cgctcaataa taagcgctct gagccatgag ccgcggaatc
     1681 gaggagaagc ctagctggct cgtccagctt ctcttggtcg cgattcgtgc ctaccagctg
     1741 ctgatcgctc cgctgctcgg gcccgtttgt cgctttcatc cgagctgttc gcgctacacc
     1801 gccgagtgct tgcgactcca cggcgcggcg cgcggcagct atctcggcgt tcgccgcatc
     1861 ctgcgctgcc acccttggaa tccaggggga tatgacccgc cgccaccgct tcgggcccag
     1921 cgatgaagca tttttaatcg aaagaaaccc gatcgaagcc cgctccgaaa ggtgctaggc
     1981 gaaggcgggt tttcttccca gcccgcggat acgacccccc aatggatcga agcaacctcg
     2041 gacgactgct gctcatgctc gcggcggtct ttttgttcat gcagtttggg ccgaagctgt
     2101 tcggcggagg cagctccgct catcagccgc tcctcccgga gaacgagcgc gccccggcga
     2161 cgcgagctcc ggagaagatc tgcgacatct gggggcctcg ctttcacgcg cagctctcga
     2221 cgcgcggcgc caccgtgaag catttccagc tcacgaccgc gaagtaccag aagaacggca
     2281 agcccatcga tctgtcgacg acgcccgatg tcgaagagcg ccggcagttg cgtttccact
     2341 ttacgaatcc ggcggcgaaa attcccgccg acaaggctca gctcgctttc gacaagctcg
     2401 atttcgagct cgccgctgca gacggaaaga gctgcgagtt cgtttaccgc gatgctcgca
     2461 ccgagctgcg cgaagtggtc aagctcaccg gtcgtcccta cgagctcgtg gccgaagcca
     2521 agctcacgaa cctctcgagc acgccgctca agcacgccct caccatcgac tcggacacgt
     2581 ggcgtaccga ccacgaagta aaggcgacga tgttccgcgt gagccccctg ctcacgcacg
     2641 tcgagtgcgt gccgaacgcc ggcaacgcga cgcgtctcac cgcgcaagat ttcgagccca
     2701 gcgacttcga gaaggccgag cattttccgc gtaacgagct gagcggcggc gacttcttcc
     2761 aggtcccggg caagcccgaa gtggcggccg tgtcgaacgc ctacttttca cacgcgctcg
     2821 tgccgctcgc gggcccggcg ccaacttgcc aactgcagat cgaagagcgc tgggacagcc
     2881 ggaagttcgc gagcaaggcg aaggacgaca atgcgggcgc cttctatcgc gcgcgcttcg
     2941 cgtatccctc gcgcgagctc gcgccgaacg agtcgacgca gtactcggtg ctcacctaca
     3001 tcggtcccaa ggagcggagt gttctggccg acgccggcgg cggacagcac cggttgatcg
     3061 agctcatcga ccttgggttc ttttcagcca tcgcgaagat cctggtggcg ttcctgctca
     3121 aggtgcacag cgtcatcccg aactggggcg tggcgatcat cgtgctcacg atcaccgcgc
     3181 gaagcttgct gttcccgctc gcgctgcccg gcatcaaggg catgatcaag atgcgcgagc
     3241 tcaagccgga aatcgacgcc ttgaacgagc gcttcaagga cgacccacag caaaagggtc
     3301 tcgctcagat ggagctctgg cggaagcaca acgtgaaccc gctgaagggg tgcttgccgc
     3361 agctcgcgtc gatgccagtc tggttcgcgc tctacacgac gttacagacg gcggtcgaac
     3421 tctataacat cccgtttctg tggttccccg acctgtcggc ggccgacccc ctgtacatcc
     3481 tccccttcat catcggcacc acttcatttt ttcagcaaaa actgatgccg atgcagggcg
     3541 accccgccca gcagaagatg atgctgtact tcatgccggc catgttcact gttttcatgc
     3601 tgtttctgcc cgccggcctc ggggtataca tgttcacgaa cggcctgctc ggaatcctcc
     3661 agcagcaagc cgtcgaacga cacgtgcgtc ggagcgttcg cccaggtggt ggctctggct
     3721 ccggtgacat caaggtgaaa gtgatagagg actcgggatc gaacgcgagc aacaagaagg
     3781 aaaagggcgc ggcgaaggct ggccgctcct cctctggctc cgacgacgac tcacgactcc
     3841 tcggggaagg caaagcgtaa gatgagcgac acccaggact tctcggacga gggttgggac
     3901 aacgccgacc cggacgcgcc gggttccgac cacgagcacg acgctccgag cgcgagcgac
     3961 aacggtgagg gcagtgcttc tgccgaaggt gccgaaacga acgggcacga cagcgcgggc
     4021 cgtcgccgtc gaagtcgcgg gcgcgggcgc gggcgtgaac acgacgat
//
LOCUS       DASTKS010001284         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_206580, whole genome shotgun sequence.
ACCESSION   DASTKS010001284 DASTKS010000000
VERSION     DASTKS010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206580"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFQ35_27510"
     CDS             complement(<1..217)
                     /locus_tag="VFQ35_27510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934494.1"
                     /translation="MSATSFNRYGMWAIYRFEMARFGRTLVQSVITPVMTTSLYFIVF
                     GSAIGPRMSQVGGVAYGSFIVPGLIMLS"
     gene            complement(214..1152)
                     /locus_tag="VFQ35_27515"
     CDS             complement(214..1152)
                     /locus_tag="VFQ35_27515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9934495.1"
                     /translation="MPPILSIKGLTKTYASGLNALKRVDLTIEQGEIFALLGPNGAGK
                     TTLINIVCGIVTPSSGTAEVAGHDIQRDYRRARAEIGLVPQELSTDAFETVTATVNFS
                     RGLFGKPADDARVETVLRQLSLWEKRKDKIMELSGGMKRRVMIAKALSHDPSILFLDE
                     PTAGVDVELRRDMWALVRGLRENGVTIILTTHYIEEAEEMADRVGIITKGELILVEEK
                     SKLMTKLGKRRLTLNLASPLAALPPELSDWRLELKAQGHELEYSFDAKDGKADVPGLL
                     NRLGALGIGFKDLSTHESSLEEIFVSLVHERKSEAP"
     gene            complement(1313..1534)
                     /locus_tag="VFQ35_27520"
     CDS             complement(1313..1534)
                     /locus_tag="VFQ35_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934496.1"
                     /translation="MNSKFAIIAFCSALFLACGGNKKPADGPAENAGEKVDEAAKDAE
                     DSAEKASEKAGDAAEDAGDKVKEKTKDED"
     gene            complement(1718..>2869)
                     /locus_tag="VFQ35_27525"
     CDS             complement(1718..>2869)
                     /locus_tag="VFQ35_27525"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET9934497.1"
                     /translation="ARGPRGYDEINLAQPGNFGWPHCIGQRLAYPEYDFATGQLGAPF
                     DCSGTVAPVLAYDYGTPTYPPLGVGILDNGTFVGRAAIAGAVYRAPAGATRGWPARFD
                     GRLLMADWTRNIVALADVDAAGSLRSLERLLPSEDFRRPIDFEVAPDGSVYVLEYGSD
                     FWGNNPDAQLSRIEYAEFGRLAPMASLTASANNGPAGTVIHFSATGSKPFSPTEYIVD
                     YEWDFDGDGETDVHASELDRRFSKSGGYVVSLRVTSSNGLKSFPIAQSIVIGNTPPSA
                     RIMAPARGTRLARGADVLLRGEASDAEDGVAPCDKLVWNVSLVHNTHAHPVATLTGCE
                     AHFVPDVAGHQDEGLLAYAVELSYTDRGGPGGEPALTGRDGIQYDVDVH"
BASE COUNT          491 a          921 c          914 g          543 t
ORIGIN      
        1 gcgagagcat gatgaggcct ggcacgatga aagagccgta ggcgacccct cccacctggg
       61 acatgcgcgg cccgatcgcg gacccgaaga cgatgaagta gagcgacgtc gtcatgaccg
      121 gcgtgatcac gctctgcacc agcgttcgcc cgaagcgcgc catctcgaag cgatagatcg
      181 cccacatgcc gtagcggttg aaggaggtcg cgctcacgga gcctcgctct tccgctcgtg
      241 gaccaagctc acgaaaatct cctccagcga gctctcgtgc gtgctcagat ctttgaagcc
      301 gatgccgagc gcgccgaggc gattgagaag accgggaacg tcggccttgc cgtctttggc
      361 atcgaatgag tattcgagct cgtggccttg tgccttcaat tcgagccgcc agtcggagag
      421 ctccggcggc aacgcggcga gcggagaggc taggttcaac gtgagtcgac gcttgccgag
      481 cttggtcatg agcttcgact tctcttcgac caagatgagc tcgcctttgg tgataatgcc
      541 gacccggtcg gccatctctt ccgcttcttc gatgtagtgc gtcgtcagaa tgatggttac
      601 cccgttttcg cggagaccac gcacgagcgc ccacatgtca cgtcggagct cgacgtcgac
      661 cccagccgtc ggctcgtcca aaaagaggat gctcggatcg tgcgagagcg ctttcgcgat
      721 catcacgcgc cgcttcatgc ctccggagag ctccatgatc ttgtctttgc gcttttccca
      781 gagggaaagc tgccgcaaga cggtttcgac ccgtgcgtcg tcggcgggct ttccgaacaa
      841 gccacggctg aagttcacgg tcgcggtgac cgtctcgaac gcgtcggtcg agagctcttg
      901 cgggacgagc ccgatctcgg cgcgcgcccg gcgatagtcg cgctggatgt cgtgaccggc
      961 gacctctgcc gtgccgctgc tgggcgtcac gatgccgcac acgatgttga tgagggtcgt
     1021 cttcccggcg ccgttcggcc cgagcagggc aaagatctca ccttgctcga tggtgagatc
     1081 cactcgcttc agcgcgttca ggccggaggc gtaggtcttg gtgagccctt tgatggatag
     1141 gatcgggggc atgccagagg ccaccatagc gtcgactcgc ccgtgtcgcg cgaattaggt
     1201 cgagggcttc gctggttctt gggccggagc aagcgccact ctgtcggcgt tggtcgcgaa
     1261 agctccggcg cgcgcggaac ccgagcgcgc tgccggagcc ccttgaacgc gctcagtcct
     1321 cgtccttggt cttttccttg accttgtcgc cagcgtcctc ggccgcgtcg ccagcctttt
     1381 cgctcgcctt ttcggcgctg tcttctgcgt ccttggccgc ctcgtcgacc ttctcgccgg
     1441 cattctcggc ggggccatcc gcaggtttct tgttgccgcc acaagcgagg aagagagctg
     1501 agcagaaagc gatgattgcg aatttggagt tcatctcgag tcgtcgtcct ttcgaggaca
     1561 cagccgtgga tctcggcgtg tgtcagtcgc cgaaagcaag tctcgcgccg caccgacatt
     1621 tacgagtaat ccgcagcgcg cacgccatga cgctgcagcc aattcgcccc acgcccgcgc
     1681 gcgatgcccc agcgccgttc tcgccgcccg agcggcttca gtgcacgtcc acgtcgtatt
     1741 gaattccgtc gcgcccggtg agcgccggct cccctccagg ccccccgcga tccgtataag
     1801 agagctcgac cgcgtatgcg agcaaacctt cgtcttgatg accggcaacg tccggaacga
     1861 agtgtgcttc gcagccggtc aacgtggcaa ccggatgcgc gtgggtgttg tgaacgaggc
     1921 tcacgttcca gacgagcttg tcgcacggcg cgactccgtc ttcggcatct gaagcctctc
     1981 cacgtagcag gacgtcggcg ccgcgagcca aacgagttcc gcgcgccggc gccatgatgc
     2041 gcgcgctcgg cggcgtgttg ccaatcacta tcgactgcgc aatcggaaag ctcttcaggc
     2101 cattgctgga agtcacgcgc aacgacacga cgtagccgcc gctcttcgag aagcgacggt
     2161 cgagctcgga ggcgtgaacg tcggtctcgc cgtcgccatc gaagtcccac tcgtagtcga
     2221 cgatgtattc ggtgggcgaa aagggcttcg agccggtcgc ggagaagtga atgacggtgc
     2281 cagccgggcc gttgttcgcc gaagcggtca ggcttgccat cggagcgagc cgaccaaatt
     2341 cggcgtattc gatgcggctc agctgggcgt ccgggttgtt tccccagaag tcactgccgt
     2401 actcgagcac gtacacggag ccgtctgggg cgacctcgaa atctatcggg cgacgaaagt
     2461 cctcgctcgg caagaggcgc tccaagcttc gaagcgagcc tgccgcgtcg acgtcagcca
     2521 acgccacgat gttgcgtgtc cagtctgcca tcaacaagcg cccgtcgaag cgtgccggcc
     2581 accctcgggt tgcgccggct ggcgctcgat acacggctcc cgcgatggca gcgcgaccga
     2641 cgaaagtacc gttgtcgagg ataccgacac cgagcggggg gtaagttggg gtgccgtagt
     2701 cgtacgcgag caccggcgcg accgtgccac tgcagtcgaa gggcgccccg agctgtccgg
     2761 tggcgaagtc gtattcgggg taagcgagcc gctgcccgat gcaatgcggc caaccgaagt
     2821 tgccgggctg tgccaggttg atctcgtcgt aaccgcgcgg cccgcgcgc
//
LOCUS       DASTKS010001285         6248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_651892, whole genome shotgun sequence.
ACCESSION   DASTKS010001285 DASTKS010000000
VERSION     DASTKS010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651892"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="xseA"
                     /locus_tag="VFQ35_27530"
     CDS             complement(<1..931)
                     /gene="xseA"
                     /locus_tag="VFQ35_27530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9934498.1"
                     /translation="MSGGPSDPIIISVGELGRRLRRVVEGASAGFWVRGEVASLKRAP
                     SGHCYFTLKDRDEDACVECVMYKFDAQRALKHLADGAEVQLFGKATVWAPRGRLQFVA
                     QKLQPAGRGALLLALEALKNKLTAEGLFEPSRKRPLPADPRVVGVVTSATGAAFHDIR
                     TVAFRRGGIVLVLSPALVQGDDAPRSLVVALERIERYPGVDVVIVGRGGGSGDDLMAF
                     NDERVVRKIAALKVPVVSAVGHEVDVSLSDLAADVRAATPSQAAELVVPDGRARRVAL
                     MRTQSALARAMQSILVSERARLDRRRARLGDPRF"
     gene            1119..2597
                     /locus_tag="VFQ35_27535"
     CDS             1119..2597
                     /locus_tag="VFQ35_27535"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9934499.1"
                     /translation="MFRKVLIANRGEIAIRISRTLRELGIESAGVFTEADRESAHLDF
                     MDQAVALGANPRSYLDVERIVDAAKKVGADALHPGYGFLSENAELAEACERRGVTFVG
                     PSASAIENMGSKQRARSLMSAAGVPIVPGGSADTLEAAKASAAAVGYPVLIKATDGGG
                     GKGMRRVDNEAELVAAFERTRSEALAAFGSDRVHIEKALIAARHVELQILGDRSGTLV
                     PFYERDCSVQRRHQKILEETPCPVLPQATLDRMAEVAIAGARSIGYYSAGTFEFLLDH
                     AGNFYFLEMNTRLQVEHPVTELVTGLDLVREMLRVAAGEAIDPEPPARRGAAIEARIY
                     AEDPSQGFLPSPGIIARLSEPSGPFVRVDSGVRQGSRVSSEYDPLLAKLSVWGSDRDH
                     ALSRLRRALSEYVVTGVETNLSFLQAVAADPEFGAGRYDTEFVGRRRDLSNAPPLDDD
                     AKLELALALSALSARSAGARSAEQTSPDAPSPWVLAERARLR"
     gene            complement(2647..3627)
                     /locus_tag="VFQ35_27540"
     CDS             complement(2647..3627)
                     /locus_tag="VFQ35_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934500.1"
                     /translation="MQTRVRLGELLVEANILGREQLEEVLALQKTDGRRLGTLLVEAG
                     LVTETQVTQILSQQLSVPWVSLYHIDFSRQLLNLVPQEIAEKYCLVPIFVRRVRGQGE
                     TLYVAMDDPSDETAHAAVAQYAGLAVRAMIAPPSDIRSAIRVYYGAGRDTASLSEAVA
                     ELETELANRMTPVMAEPTHYAPAQAPPAPSSLKPRASSKPPASISTPPMEEPPDSSPT
                     IEAREVSMPAPKRGRPRKMVTLTLLDGTQLNLPAKRPHEPSDPAPKDASELTARDLIA
                     ALRAVSHGADATEILGENTRWEALFAAVLSLLLKKQLIADWEFGEAFRKV"
     gene            3875..>6248
                     /locus_tag="VFQ35_27545"
     CDS             3875..>6248
                     /locus_tag="VFQ35_27545"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934501.1"
                     /translation="MSTQPTFRICPHCGSSCAMEHQYCPACGFPVGNMGSTVEDRFIG
                     KSLPGGYHILDLISVGGMGRVYRAQQSALGRTVAVKIIHPHLLSDENSALRFMTEARA
                     ASQLNHPNSVAVFDFGRTDDGQPYLVMEFLRGKDLARVAYEEGPLPFSRVVDVLRQVL
                     AALGEAHDLGIVHRDLKPENVILEPLRRGGDFVKVVDFGLAKLRADTQGPGVTSPGIV
                     CGTPDYMAPEQGRGDPIDGRSDLYAVGVILFQLLTGRLPFEAESPTQVVMMHLTVPVP
                     DPRQVAPERRIPDALVDVVMRALAKDVRKRYQDAQEFADGLKQALILAESLPPERATV
                     PPVSLVTNTVKCQSCGLVVPFSKFCGECGARLPARTGLPDTQMVPLLPLAFVAREDEL
                     NWLDERRRDASRGFACARLIGEPGAGKSRLLQELALVARGDGDAVVLTGPDPFHAEVA
                     CFALREAVRQLARLGPADIDAGQYDRARPEARAGLDDIFGRRDRENGRAPFERRAALA
                     EALRWALGVALTRSQRVLLAVDDLDDIDGPSFNAFSDIIDASPDMAVLLLVAHRPGFD
                     AEAGTRAPARVVGGLPPPAVSRLLSGRRGERIPFSDGSKSALPMYVEQVIRYSQDGGT
                     DPPTRLADLIALRVDMLEPNARRTLQALVVLGDQWSIDAMAELLPKAEELDARVQELI
                     RAGMVEEAEGKLRTSHPLLREVVLASIPVAVRRELHKKALQVCEKRGAPIEARALHAY
                     ESGDSFQALLLLEQVADRATARGDEHTEVLALRRSLEVARKEIARGELDDP"
BASE COUNT         1029 a         2148 c         2064 g         1007 t
ORIGIN      
        1 cgaagcgcgg atcgcccaag cgggcgcggc gccggtcgag ccgcgcccgt tcactcacca
       61 agatcgactg catcgctcgc gcgagcgcgc tctgcgtgcg catcaacgcg acccttcgcg
      121 ctcgtccgtc cggaacgacg agctccgccg cttgggaagg agtcgccgcg cgcacgtcgg
      181 cagccaaatc ggagagcgag acgtcgacct cgtggcccac cgcgctcacc acgggcacct
      241 tcagcgccgc gatcttccgc acgacgcgct cgtcgttgaa cgccatcaga tcgtcacccg
      301 agccaccgcc gcgcccgacg atcaccacgt cgaccccggg gtaacgctcg atacgctcga
      361 gcgcgacgac gaggctacgg ggggcatcgt ctccctgaac gagcgcgggc gagagcacga
      421 gcacgatgcc gccccggcga aacgcgacgg ttcgaatatc gtgaaacgcc gcccccgtgg
      481 cgctcgtcac gacgccgacc acgcgaggat cggccggcaa agggcgtttg cgtgacggct
      541 cgaacagacc ctcggcggtg agcttgtttt tcaaagcctc gagcgcgagc aagagcgcgc
      601 cgcgccccgc tggctgcagc ttttgtgcaa cgaactggag ccgcccgcgc ggggcccaca
      661 cggtcgcctt gccgaacagc tgcacctcgg cgccgtccgc cagatgtttc agcgcgcgtt
      721 gcgcgtcgaa tttgtacatg acgcattcga cgcacgcgtc ctcgtcgcga tccttcaggg
      781 tgaagtagca gtgaccgctc ggggcgcgct tcagcgacgc gacctctccc ctcacccaga
      841 accctgccga agcgccttcc acgacgcggc gcagccgccg gccgagctcg ccaacgctga
      901 taatgatggg atcagagggc cctccgctca cggcggggcc gggtatagca gctgccgagc
      961 gaacgcggtg cgtcgaatcg tcctcgatac gacgaatccg agcacaagaa gcatgctttt
     1021 cggcctcgga gccgcctccg ccgctctccc acacacgccc accgcccagg gttcgagacg
     1081 acaccctcgg cgcttcgtgt tacccgatgg cgcgcccgat gttccgaaaa gtcctcatcg
     1141 ccaaccgagg tgaaatcgcg atccgcattt cacgcacgtt acgcgagctc ggcatcgaga
     1201 gcgcgggagt attcacggag gcagatcgcg agagcgcgca cctcgatttc atggaccagg
     1261 cggtggcgct cggagcaaat cctcgttcct acctcgacgt cgagcgcatc gtcgacgccg
     1321 cgaagaaggt gggcgccgac gcgctccacc ccggctacgg cttcttgtcg gagaacgcgg
     1381 agctcgccga agcttgcgag cgccgcggcg tcaccttcgt tgggccctcg gcgagcgcga
     1441 tcgaaaacat ggggagcaag cagcgcgcgc gctcgttgat gagcgccgcc ggtgtgccaa
     1501 tcgtccccgg cggctccgcg gatacgctcg aagcggccaa ggcgtcggct gccgctgttg
     1561 gttatccggt gctcatcaag gcgaccgacg gcggcggtgg caaaggcatg cgccgcgtcg
     1621 acaatgaagc ggagctcgtg gcggctttcg aacgaactcg cagcgaagcg ctcgcggcgt
     1681 tcgggagcga ccgagtgcac atcgaaaaag ccttgatagc tgcgcgccat gtcgagctcc
     1741 agatcttggg cgatcgcagc ggcacgctcg tgccttttta cgagcgcgac tgctcggttc
     1801 agcggcgcca ccagaagatc ctcgaagaga cgccctgccc ggtgctaccg caggcgacgc
     1861 tggatcggat ggcggaggtc gcgatcgccg gcgctcgctc gattggctac tactcggcgg
     1921 gtaccttcga gtttctgctc gaccacgccg gcaacttcta cttcctcgag atgaacacgc
     1981 gcctccaggt cgagcacccc gtcaccgagc tcgtgaccgg gctcgatttg gtgcgcgaaa
     2041 tgctgcgcgt cgccgcgggc gaagccatcg atcccgagcc gccggcgcgg cgcggcgctg
     2101 ccatcgaggc gcgcatctat gccgaagatc cgagccaggg ctttttaccg agcccgggca
     2161 tcatcgcgcg cctctcggag ccgtcggggc ctttcgtgcg cgtcgacagc ggcgttcgcc
     2221 aaggctcgcg cgtgagcagt gagtacgatc cgctccttgc gaagctgtcg gtgtggggaa
     2281 gcgatcggga ccacgccctc tctcgcttgc gtcgcgcgct cagcgaatac gtcgtcacgg
     2341 gggtcgaaac caacctctct ttcttacaag cggtcgcggc cgaccccgag ttcggcgcag
     2401 gtcgctacga caccgagttc gtcggccggc ggcgggatct ctcgaacgcg ccgccactcg
     2461 acgacgacgc gaagctcgag ctcgccctcg ctctcagcgc gctctcggcg aggtcggctg
     2521 gggcacgaag tgccgagcaa acgtcgcccg acgcgccgtc gccgtgggtg ctggccgagc
     2581 gcgcacggct gcgctgagca cgcccgccgc gaggcatcac gaacgacctt gccggagccg
     2641 agaggctcac accttgcgaa acgcttcgcc gaactcccaa tcggcgatga gctgtttttt
     2701 caagagcagc gacaaaaccg cggcgaacag cgcctcccag cgcgtatttt ccccgaggat
     2761 ttcggtcgcg tccgcgccgt gagaaaccgc gcgcagcgcg gcgatgagat cgcgggcggt
     2821 gagctcggaa gcatccttcg gtgccggatc ggagggttcg tgcgggcgct tcgcgggtaa
     2881 gttcagctgc gttccgtcga gcagcgtcaa cgtcaccatc ttgcgtgggc gaccgcgctt
     2941 cggcgccggc atcgagacct cacgcgcctc gatcgtgggg ctgctatcgg gcggctcttc
     3001 catcggcggc gtcgaaatgg atgcgggagg cttcgacgac gcgcgcggct tcagcgagga
     3061 cggcgcggga ggtgcctgcg cgggagcata gtgcgtcggc tccgccatca ctggcgtcat
     3121 tcggttcgcg agctccgtct cgagctcggc gactgcctcc gagagcgacg ccgtgtcgcg
     3181 accagcgcca tagtaaacgc gaatggccga gcgaatgtcg gaaggcggcg cgatcatcgc
     3241 gcgcacggcg aggcccgcgt actgcgcaac ggcggcgtgg gccgtctcgt cgctcgggtc
     3301 atccatcgcg acgtacagcg tttcgccctg tccgcgcacg cggcgcacga agatcggaac
     3361 caggcagtac ttttcggcga tctcttgcgg caccaggttc aaaagctggc gagaaaagtc
     3421 gatgtggtag agcgacaccc aaggaacgct gagctgctga ctcaagatct gagttacttg
     3481 cgtttccgta acgagccctg cctcgaccag caacgtaccg agcctgcgac catcggtttt
     3541 ctggagggcg agcacttctt ccagctgctc ccggccgaga atgttggcct ctacgagcag
     3601 ttccccgagt cgcacacgcg tctgcatgcc cagaatgcta gcagttggtc cggctcggag
     3661 gatggaagtt cccttgctcc gcgccctagt agcgagcgcc cgccgctcgg ccgcacggtg
     3721 aacgggcggg gtgagcctgc gtgcgccacc cacaaaaacc gccgttcacg cgtcaaggct
     3781 ggttcaagcc cgttacctcg gaataaactc cggtcagcgc cttggcgacc aggatggtag
     3841 cggggccggc gccagagtct ctcctccgtc ctcgatgtcg acccagccca ccttccggat
     3901 ctgcccgcac tgcggctcct cgtgcgccat ggagcaccaa tattgcccgg cgtgcggatt
     3961 tccggtcggc aacatgggct ccaccgtcga agatcgcttc atcggcaagt cgctgcccgg
     4021 cggatatcac atcctcgatc tgatcagcgt cggcggcatg gggcgcgtgt accgggccca
     4081 acaaagtgcg ctcggacgca cggtcgccgt caagatcatc cacccacacc tgctctcgga
     4141 cgaaaactcg gcgctgcgct tcatgaccga ggcgcgcgcg gcgagccagc tgaaccatcc
     4201 gaactcggtc gcggtgttcg acttcggacg caccgacgac ggccagccct acctcgtgat
     4261 ggagttcttg cgcgggaagg atctcgcgcg cgttgcctac gaggaaggcc cactgccctt
     4321 ctcgcgcgtc gtcgacgtgc tccgccaagt gctcgcggcg ctgggagaag cgcacgacct
     4381 cggcatcgtg caccgtgacc tgaagcccga gaacgtgatt ttggagccgc tccggcgcgg
     4441 gggcgatttc gtcaaggtgg tggacttcgg tctcgccaag ctgcgcgccg acacccaggg
     4501 gccgggcgtg acgagccccg gcatcgtgtg cggaacgccc gactacatgg cgcccgaaca
     4561 gggccgcggc gatccgatcg acggacgcag cgacctctac gcggtcggcg tcatcctgtt
     4621 ccagctcctg acgggcaggc tgccattcga agccgaaagc ccgactcaag tcgtcatgat
     4681 gcacctcacg gtgccggtgc cggatccgcg gcaggtcgcg ccggagcgtc gcatccccga
     4741 cgcgctcgtg gacgtcgtca tgcgcgcctt ggcgaaggac gtacgcaagc gctaccagga
     4801 cgcgcaggag ttcgcggacg gcctgaagca ggcgctcatc ctcgccgaga gtcttcctcc
     4861 cgagcgcgcc accgtaccgc cggtgtcgct cgtcacgaat acggtcaagt gccaaagctg
     4921 cggtttggtc gtcccgttca gcaagttctg cggcgagtgc ggagcgaggc tgcctgcgcg
     4981 cactgggctc ccagacacgc agatggtgcc gctcttgccg ctcgcgttcg tcgcgcgtga
     5041 agacgagctc aactggctcg atgagcgccg tcgcgacgcg agtcgcggct tcgcctgcgc
     5101 gcgcttgatc ggagagcccg gcgccggcaa gtcacgcttg ctgcaagagc tcgcgctcgt
     5161 cgcgcgcggt gatggcgacg cggtcgtcct caccggcccc gaccctttcc acgccgaggt
     5221 cgcttgtttc gcgctgcgcg aggcagtgcg gcagctcgct cgcttgggcc ccgccgacat
     5281 cgacgcggga cagtacgatc gggcgcgccc cgaggcgcgc gccgggctcg acgacatctt
     5341 cgggcggcgt gatcgagaga acggtcgcgc gccgttcgag cgtcgggcgg cgcttgccga
     5401 ggccctgcgc tgggcgctag gcgtcgcgtt gacgcgcagt cagcgggtgc tcttggccgt
     5461 cgacgatctc gacgacatcg atgggccgag cttcaacgcc ttctccgaca tcatcgacgc
     5521 atcgccagac atggcggtgt tgctgctcgt cgcccatcga cctggcttcg acgccgaagc
     5581 gggaacgcgg gcgccggcgc gcgtcgtcgg gggcctgcct cctcccgccg tgtcgcgctt
     5641 gttgagcggt cgtcgcggcg aacgtattcc cttcagcgac ggcagtaaga gcgcgctgcc
     5701 gatgtacgtg gagcaggtga tccgctactc gcaagacggc ggtacggatc caccgacccg
     5761 cctcgccgac ttgatcgcgc tgcgcgtcga catgctcgag ccgaacgcgc gccgcacgtt
     5821 gcaggcgctc gtcgtgctcg gtgaccagtg gtccatcgac gcgatggcgg agctcctgcc
     5881 caaagcggaa gagctcgacg cgcgcgttca ggagctcatc cgcgcgggaa tggtggagga
     5941 ggcggagggc aagctccgaa cctcgcaccc gctgctgcga gaagtcgtgc tcgccagtat
     6001 tccggttgcg gttcgccgcg agctccacaa aaaggcgctg caagtctgtg agaagcgcgg
     6061 cgccccaatc gaagcacggg cccttcacgc ctacgagagc ggcgactcct tccaagcctt
     6121 gctcttgctc gagcaggtcg ccgaccgagc gaccgcgcgc ggcgacgaac atacggaggt
     6181 cctcgcgctg cggcgtagcc tcgaggtcgc tcgcaaggaa atcgcgcgcg gcgagctcga
     6241 tgacccgc
//
LOCUS       DASTKS010001286         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_206667, whole genome shotgun sequence.
ACCESSION   DASTKS010001286 DASTKS010000000
VERSION     DASTKS010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206667"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFQ35_27550"
     CDS             <1..512
                     /locus_tag="VFQ35_27550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934502.1"
                     /translation="KPAIALVVDEVEPPRSLVEGLTRFGHEVRLASDVATARSLFRAC
                     TPALVLLELHVGQASGLDLLAEFRLVSPHTRFVIVTMYGSIESARLAMASGADDYFTK
                     PVTARELLPSVAACADPMLPSGPAFLGVQAMRKQYIEQVLAQCGSIAQTSRLLRVERR
                     SLRRMLRRA"
     gene            579..1604
                     /locus_tag="VFQ35_27555"
     CDS             579..1604
                     /locus_tag="VFQ35_27555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9934503.1"
                     /translation="MLSDRKSPLPCTLTASNGQAVSRIGLGCMGMSEFYGARDDEASV
                     RVVVSAFELGYRHFDTADFYGQGHNEELVSRFLGWVGARREEVFVATKCGLRRAPGPK
                     PGVEIDSRPEYVRAACDASLRRLGTEYIDLLYLHRRSTSVPIEETVGALAELVVSGKC
                     RHVGLSEVSLATLRRAHATHPITALQNEYSLWSRDVENGVLQTTRELGIALVAYSPIG
                     RGFLSGALTRQKTEEPGDLRAMLPRFANGNFEQNALLLEELGRVASELGGATMSQVAI
                     AWVLSRAPNVYAIPGTRRLEHLQANAQAASLPLSDGQAERLAAAFAPDAICGSRYPEA
                     LLATIGT"
     gene            complement(1568..>2721)
                     /locus_tag="VFQ35_27560"
     CDS             complement(1568..>2721)
                     /locus_tag="VFQ35_27560"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9934504.1"
                     /translation="ESIGAKLEGWDYPMHFLLGDFPLAWDRPSDQAYYYAFEETFFVI
                     VPVSAGYWRVVVKRDGPFDPAAKIEANDIRELAERYLPTGLLAGDPIWLSGAPFYMRM
                     ASSLQRGRRFLCGDAAHLFSPIGGTGMNTGMMDAFDLAWRLAYVHHGHSTAEAVLPSY
                     GEERSEAIERGAAAADAATRLIARIERAPERIAPMLPSLRGRRLLRRVFPFQQAGLGL
                     EYSRSSIIRGGNAAGAPRPGTMCLGLLELRRQIEVRTGKRTPLLETSVLIFVAAESLS
                     AREVKLRAWLSDLSRFGGASLGVHIIAIGRKLPARSSATLAIDDITTLRRLGARDGAV
                     LAIRPDGIIAFSGGLDDRQALLEHLGRWLVSASAEVTAQVPMVASNASG"
BASE COUNT          472 a          904 c          882 g          463 t
ORIGIN      
        1 caaagcccgc gatcgccctc gtcgtggacg aggttgagcc gccgcgctct ctcgtcgaag
       61 ggttgacccg gttcggacac gaggtgcggt tggcgagcga cgtcgcgacg gcgcgttctc
      121 tgttccgagc gtgcacgccc gccctcgtcc tactggagct ccacgtggga caggcttcgg
      181 ggctcgatct attggcggaa ttccgcctcg ttagtcccca caccaggttc gtcatcgtca
      241 cgatgtacgg ctcgatcgaa tcggcacgct tggccatggc ctcgggagct gacgactact
      301 tcacgaaacc ggtcaccgcg cgcgagctat tgccgtcggt cgcagcgtgc gcggacccga
      361 tgctgccttc ggggcccgca ttcttgggcg tccaggccat gcgcaagcag tacatcgagc
      421 aagtgttggc ccaatgtggg tccatcgctc agacatcgcg gctcttgcgt gtggagcgcc
      481 ggtcactccg gcgcatgctg cgccgcgcct gatgcgcgcg gattcggccg cttgctctcg
      541 attcacggcg ccctcatcgc caaaagaaag gtcaagacat gctctccgat cgaaagtcgc
      601 ccctgccctg cacgctgacc gcctcgaacg gccaagccgt atcgcgcatc gggctcggtt
      661 gcatggggat gagcgaattt tatggtgcgc gcgacgacga agccagcgtg cgcgtggtgg
      721 tttctgcgtt cgagctcggg tatcgccatt tcgataccgc cgacttctac gggcagggtc
      781 acaacgagga gctcgtttcg cggtttctgg gttgggtcgg cgcgcgccgc gaagaggtct
      841 tcgtggccac caagtgcggg ctgcggcgcg ctccagggcc caagcctggc gtggaaatcg
      901 actcgcgccc cgagtacgtg cgcgcggctt gtgacgccag cctgcgccgg ctcgggaccg
      961 agtacatcga tctactgtat ctgcatcggc gatccacgag cgttccgatc gaagagactg
     1021 tcggtgccct cgctgagctc gtcgtgagcg gcaagtgccg gcacgtcggg ctctccgagg
     1081 tgtcgctcgc gacgctcaga cgcgcccacg cgacccaccc gatcaccgct ctccagaacg
     1141 aatactcgct ctggagccgc gacgtggaga acggcgtact tcaaacgacg cgcgaattgg
     1201 ggatcgcgct cgtcgcctac tcgccgattg ggcgcggctt tttgagcggc gctctgaccc
     1261 gacaaaagac ggaggagccg ggagatctac gcgccatgct gccgcgcttc gccaacggca
     1321 atttcgagca aaacgcgctg ttgctcgagg agctcggccg cgtcgcgagc gagctcggtg
     1381 gcgcgacgat gtcacaggtt gcaatcgcgt gggtattgtc gcgcgcgccg aacgtctacg
     1441 ccattccggg cacgcgccgc ctagagcacc tgcaggccaa tgcgcaagcc gcgtcactac
     1501 cgctgagcga tggtcaagcc gagcgcctcg ctgccgcatt cgcgcccgat gcaatctgcg
     1561 gctcccgcta tcccgaagcg ttgctcgcga ccatcggcac ttgagccgtc acttccgcgg
     1621 aagcggagac caaccaacga ccgaggtgct cgagcagcgc ctgccggtcg tcgaggccgc
     1681 ccgaaaaagc gatgatgccg tccggtcgga tcgcgagcac ggcgccatcg cgcgccccca
     1741 gccgacgcag agtggtgatg tcgtcaatcg cgagcgtcgc gctcgagcga gcagggagct
     1801 tgcggccgat cgcaatgatg tgcacgccga gcgaggcgcc gccaaagcgc gagagatcgc
     1861 ttaaccacgc gcggagcttg acctcgcgag ccgaaaggct ttcggcggcg acgaaaatca
     1921 agaccgaagt ctcgagcagc ggggtgcgct ttccagttcg aacctcgatt tgacggcgga
     1981 gctccaacag accgagacac atggtccccg gcctcggcgc tcccgccgcg ttcccgccgc
     2041 gaatgatgct agagcggctg tactcgaggc cgagccccgc ctgctggaac gggaacacac
     2101 gacgcaagag ccttcgcccg cgcaagctcg gaagcatggg tgcgattcgc tcgggagccc
     2161 tctcgatgcg cgcaatcagc cgggtcgccg catccgctgc cgcagcgccg cgttcgatgg
     2221 cctcactgcg ctcttctccg tagctcggga ggacggcttc ggccgtggaa tggccgtggt
     2281 ggacgtacgc gagacgccaa gcgaggtcga acgcatccat catgccagtg ttcatgcccg
     2341 tgccgccgat cggactgaaa agatgcgccg cgtccccgca aaggaatcgc cgcccgcgct
     2401 ggagcgagct cgccatccgc atgtaaaagg gcgcgcccga gagccagatc gggtcgcccg
     2461 caaggagccc ggtggggagg tatcgttcag cgagctcgcg gatgtcgttc gcttcgatct
     2521 tcgcggctgg gtcgaagggg ccgtcacgct ttacgaccac ccgccaatag cccgcgctca
     2581 ccggaacgat gacgaagaac gtctcttcga acgcatagta gtatgcctga tcgctcggac
     2641 ggtcccaggc cagtgggaaa tcacccagca gaaagtgcat cgggtagtcc caaccttcga
     2701 gcttggcccc gatgctctcg c
//
LOCUS       DASTKS010001287         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1994747, whole genome shotgun sequence.
ACCESSION   DASTKS010001287 DASTKS010000000
VERSION     DASTKS010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994747"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFQ35_27565"
     CDS             <1..917
                     /locus_tag="VFQ35_27565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9934505.1"
                     /translation="GRARIHALLDDLGGLALFSEIVEQRSFTAGARRSGTTTSAASKR
                     IANLERKLGVRLIERTTRRAVPSEAGLLLYERCQALLEQASELEQALASHRGQLAGTL
                     RVSAPVTFGQLHIVPLVTTFCARYPELRVQLSLNDQSVDLLASGVDVAIRAGRTHDAS
                     SMSRKVGDDRRVLCATPEYLQKSGVPRVPNDLLGHRCLRHPLMAPSGGWAFITPEGSV
                     TIPVRGPIEIDNVAALREVALTSAGLTLLPAYAVGGDLRSGRLVSVLDAFIPKSPPFR
                     ALWLAGKQPVPRVVAFVDYIASELPKRL"
     gene            complement(918..1661)
                     /locus_tag="VFQ35_27570"
     CDS             complement(918..1661)
                     /locus_tag="VFQ35_27570"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9934506.1"
                     /translation="MTPSPVVLITGASSGIGLGLAQSYAASGAKLVLNARNAHKLAAA
                     AEDLRGHSGLELALGNVGESGVAERLVATARERFGRLDVVVNNAGIFTPKAIADYTEA
                     DVDAFLAINLRGPILLSQAAVRQFRRQTGGGTIVNVTSSISMAPLAMVPASVPNSTKG
                     ALNAFTRSLALELAPEGIRVNAVAPAMIRTPLHGTTSDDYAALGKLHPMGHIGEISDV
                     VMAVRYLAEQPFTTGTVLTVDGGATLGHW"
     gene            complement(1658..1867)
                     /locus_tag="VFQ35_27575"
     CDS             complement(1658..1867)
                     /locus_tag="VFQ35_27575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HET9934507.1"
                     /translation="MPYVNIKVTREGVTPAQKEKLIRGVTGLLVEILNKSPSSTMVVI
                     DEIETDNWGFDNETVTTLRKRERAL"
     gene            2276..2935
                     /locus_tag="VFQ35_27580"
     CDS             2276..2935
                     /locus_tag="VFQ35_27580"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET9934508.1"
                     /translation="MPHDADALVDRFVDAQLRGDRREALRLVLEEGLGSGASATALEL
                     EVIQPAQRKIGELWAANRIGIAEEHVATAIANLVLTALYAHLPRRDTNDRRVLVACVE
                     GELHELGARISSDFLEAAGFDVTFAGANVPTHELVNLVRQRRSELVALSATMTFHIPA
                     LRRAVGELRAAFGHDFPIAVGGRACDVSPELVAELGVTVYGTDAAALVDGALRALAVA
                     A"
     gene            2932..>4406
                     /locus_tag="VFQ35_27585"
     CDS             2932..>4406
                     /locus_tag="VFQ35_27585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934509.1"
                     /translation="MTLNAFREARLRDSREFELVCSEGGLILEVDERGERLLGECVGQ
                     PLRAFAVPETETKLDAVLAFPAATPENGWEVSLLARGKPVTAALRATRHADQVFVLGS
                     LLPQEHASILMHMNETIGELADRQRETERQKRELMRLNRELSESNRGLLSLHAELDEK
                     SDMLRRAADVKGRVVANVSHEFRTPLNSILGLSALLLERVDGDLTPEQEKQLRFIRHS
                     ASSLSELVNDLLDLSKLEAGKLMIRPRRFEIQELFAALRGMMRPLLSNSNVELVVESG
                     ASLPAMETDEGKISQILRNLVSNSVKFTEQGTIKVGARAASDQMVEFFVEDTGIGIAP
                     ENIERVFDEFSQIDSALQRQVKGTGLGLALSRKLAEVLGGTLTAESALGKGSVFRLRV
                     PVRHEEVEVLAELTQQSQKLDPRRAPILVVEDDRQTLFLYEKYLKNSGFQVVPARSID
                     DARLVLERVKPAAIVLDVMLEGEATWGFLSELKESAETHEIP"
BASE COUNT          739 a         1375 c         1479 g          813 t
ORIGIN      
        1 gaggccgcgc ccgcattcac gctctgctcg acgatctcgg ggggctcgcg ctcttcagtg
       61 agatcgtcga gcagcgctct tttacggccg gcgctcgtcg aagcggcacg acgacgtccg
      121 ccgccagcaa gcgcatcgcg aacctcgagc gcaagctggg agttcgtctc atcgagcgta
      181 ccacgcggcg cgcagtcccg agcgaagcag ggctcttgct ctacgagcgt tgccaggcgc
      241 ttctagagca ggcctccgag ctcgagcaag cgctcgcctc acatcggggg cagctcgcgg
      301 gtaccttgcg ggtgagcgct cccgtgacct tcggtcaact ccacatcgtg ccgctcgtca
      361 ccacattctg tgctcgctac ccggagttgc gcgtccaact gtcgctgaat gatcagagcg
      421 tcgacttgtt ggcgagcggc gtcgacgtgg cgattcgcgc gggtcgcact cacgacgcga
      481 gctcgatgtc gcgtaaggtc ggtgacgatc ggcgcgtgct ctgtgcgacg ccggagtacc
      541 tgcaaaagtc cggggttcct cgcgtgccga acgatctttt gggacatcgg tgcttgcggc
      601 acccgctgat ggcgccctcc ggcggatggg ctttcatcac gccggagggc tccgtgacca
      661 taccggttcg aggccctatc gagatcgaca acgtcgccgc tctgcgcgag gttgctttga
      721 cgtcggccgg tctcacgctg ctgccggcgt atgcggtggg aggcgacttg cgctcggggc
      781 ggctagtttc cgtgctcgat gcattcattc ccaagtcgcc gccgtttcga gccttgtggc
      841 tggcgggaaa gcagccggtg ccgcgcgtcg tggcgttcgt cgactacatc gcgagcgagc
      901 taccgaaacg gttgtgacta ccaatgcccg agcgtggcac cgccgtccac ggtgagcacg
      961 gtcccggtcg tgaaaggctg ctccgccaag tagcgcaccg ccatgacgac gtcgctgatc
     1021 tctccgatgt gccccatcgg gtgaagctta ccgagcgccg cgtagtcgtc cgatgtggtg
     1081 ccatggagcg gtgttcggat catggccggc gcgactgcgt tgacgcggat cccttccggc
     1141 gcaagctcga gggccaggga gcgcgtgaag gcgttgagcg cgcccttggt cgagttcggc
     1201 acgctcgccg ggaccatcgc gagcggcgcc atcgatatcg aagaggtgac gttgacgatg
     1261 gtgcccccgc ccgtctggcg acggaactgc cggaccgcgg cttggctcag tagaatcggc
     1321 ccacgcaggt tgatggccaa gaaggcgtcc acgtccgcct ccgtgtagtc ggcgatcgct
     1381 ttcggggtga agatgccggc gttgttgacg acgacgtcga gccgcccgaa gcgttcgcgc
     1441 gcggtcgcga ccaagcgctc cgcgacgccg ctctctccga cgttcccgag ggcgagctcg
     1501 agcccggagt ggccacgtag gtcttccgcg gccgcagcga gcttgtgcgc gttgcgcgcg
     1561 ttgagcacga gcttcgcgcc gctcgcggcg tagctttgcg ccaggccgag cccgatgccg
     1621 ctcgacgccc ccgtgatcag gacgaccggt gacggggtca tagggctcgc tcccgcttgc
     1681 gaagcgtcgt cacggtttcg ttgtcgaaac cccagttgtc ggtctcgatc tcgtcgatca
     1741 cgaccatggt agagctcgga gacttgttca agatctctac gagcagaccg gttactccac
     1801 ggatgagctt ttctttctgt gcaggggtaa cgccttcgcg cgtcaccttg atgttcacgt
     1861 acggcattcg tcctccaatg ccactcggtc gagcggcgcc ccgagaacgt gagtcagccg
     1921 cgccccgtcg atcagtcctc cccgacgcca gatattgttg ccgcgaggga aacaatcgcg
     1981 tccgccagcg ctgtgccgcg acgcgttgct tcgcgtgccg gtcgactagc acgagagtcg
     2041 agtacgcatc tcaggtcctt caccgcacgt tcccgtcaac caggaggacg agatacacgg
     2101 tatgcgccgc tcgttccgcg aagcctccga gagctcgcat cgagaaaagg gacagagaga
     2161 ccgtcgttct cgtgaacgaa cgctgtggca actctgcgga gagtggcaat tcgcctcagt
     2221 tggggaggcg atcaggttct ggctaacgaa ggggctcaaa ttatactgac gacgagtgcc
     2281 ccacgatgca gacgctttgg tcgataggtt cgtcgacgcg caacttcgcg gggaccggcg
     2341 tgaggctctg cgtctcgtcc tcgaagaagg gctcggcagc ggtgcgtcgg cgacggcgct
     2401 agagctcgag gtgattcagc ccgcgcagcg aaaaataggg gagctctggg cggccaatcg
     2461 gatcgggata gccgaggagc acgtcgcgac ggcgatcgcg aacttggtcc tcaccgcgct
     2521 ctacgcccat ttgcctcggc gtgacacgaa cgaccggcgt gttctcgtcg cgtgcgtcga
     2581 gggagagctg cacgagctcg gcgcgcgcat ttcttccgac ttcttggagg cggcagggtt
     2641 cgacgtcacg ttcgccggag ccaacgtacc gacgcacgag ctcgtgaact tggtgcggca
     2701 gcggcgctcc gagctcgtcg cattgtccgc gacgatgacg tttcatattc cggctttgcg
     2761 ccgtgccgtt ggcgagttgc gagcggcttt tggtcacgat tttccgatcg cggttggcgg
     2821 tcgtgcttgc gacgtgagcc cggagctcgt ggcagagctc ggcgtcaccg tctacggaac
     2881 cgacgcggca gcgttggtgg atggggcgct gcgggcgctc gcggtggcgg catgaccttg
     2941 aatgcgttca gagaggcgcg gctgcgcgac agccgtgagt tcgagctcgt gtgctcggag
     3001 ggcgggctca tcttggaagt ggacgagcgg ggtgaacgct tgctcggcga gtgcgtcggg
     3061 cagccgctgc gtgcgtttgc ggttccggag acggaaacca agctcgacgc ggtgctcgcc
     3121 ttccccgccg cgaccccgga gaatgggtgg gaggtcagcc tgctcgctcg cggcaagccc
     3181 gtcacggccg cgctgcgtgc aacgcgccat gcggaccagg tcttcgttct cggcagcctc
     3241 ttgccccaag agcacgcgag catcttgatg cacatgaacg agacgatcgg cgagctcgca
     3301 gaccgtcagc gcgagacgga gcggcaaaag cgcgagctca tgcgcctgaa ccgagagctc
     3361 agcgagtcca atcgaggtct actcagccta cacgccgagc tcgacgagaa gagcgacatg
     3421 ctgcggcgcg cggccgacgt gaaaggccgc gtggtcgcga atgtgagcca tgagtttcgc
     3481 acgccgttga actccatcct ggggctttcg gccttgctcc tcgagcgcgt cgacggggac
     3541 ctcacgccgg aacaggagaa gcaactccga ttcattcgtc actcggcatc ttcgctgagc
     3601 gagctcgtga acgacctctt ggatctttcg aagctcgagg cgggcaagct gatgatccgt
     3661 ccgcggcgct tcgagattca ggagttgttc gcggcgctgc gcgggatgat gcggccgctg
     3721 ctctcgaact ccaacgtcga gctcgtcgtc gagtccggcg cgagcttgcc ggcgatggag
     3781 accgacgaag gcaagatttc gcaaatcctg cgcaacttgg tgtcgaactc agtcaaattc
     3841 accgagcaag gcacgatcaa ggtcggcgcg cgcgccgctt cggaccagat ggtcgagttc
     3901 ttcgtcgaag acacgggcat cggcatcgcg cccgagaaca tcgaacgagt cttcgacgag
     3961 ttttcccaga tcgacagcgc cttgcagcgc caggtgaaag ggacgggtct cgggcttgcg
     4021 ctctctcgca agctcgccga ggtgctcggc ggaacgctga ccgccgagag cgcgctcggt
     4081 aaaggctcgg tcttccgact gcgggttccg gtcaggcacg aggaggtgga ggtgttggcc
     4141 gagctcacgc agcaaagcca gaagctcgat ccccggcgcg ctcccattct ggtggtggag
     4201 gacgatcggc agaccctgtt cctctacgaa aagtacctca aaaactcggg cttccaagtc
     4261 gttccggccc gcagcatcga cgacgctcgc ctggttctcg agcgcgtgaa gccggcggcg
     4321 atcgtgctcg acgtgatgct cgaaggggag gccacgtggg gatttttgag cgagctcaaa
     4381 gagtcggccg agacccacga aatacc
//
LOCUS       DASTKS010001288         7990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_428460, whole genome shotgun sequence.
ACCESSION   DASTKS010001288 DASTKS010000000
VERSION     DASTKS010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428460"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VFQ35_27590"
     CDS             <1..1395
                     /locus_tag="VFQ35_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HET9934510.1"
                     /translation="PTAPGFAPPVASSPSAPSFPTSAATPVRAASDVPYGGELSLPEH
                     QTPDLRRARRLQSGIFDPYAVRRDFPILEERVNGHRLVWLDNAATTQKPRAVIDRLSY
                     FYVHENSNIHRGAHTLAARATDAYEAARESVRRFLNAPSRDGVVFVRGATEAINLVAQ
                     AWGRRHVSAGDEIVVTWLEHHANIVPWQQLCAEKGARLRVAPVDDHGQIRLDEFEKLL
                     GPRTRLVSLSQVSNALGTITPAREMVQIAHRYGAKVLVDGAQAVSHMPVDVQALDCDF
                     YVFSGHKVFAPTGIGALFGKADVLESMPPWQGGGNMIADVTFERTVYQAPPFKFEAGT
                     GNIADAVGLGAALDYVSALGMETISRYEHELLVYGTERLKEVPGLHMVGTAPDKAGVL
                     SFVLDGQRTEDVGGALNQEGIAVRSGHHCAQPILRRFGLEASVRASLAIYNTCEDIDA
                     LTDALKRIQASRPS"
     gene            complement(1400..2041)
                     /locus_tag="VFQ35_27595"
     CDS             complement(1400..2041)
                     /locus_tag="VFQ35_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9934511.1"
                     /translation="MSEARRVSDCDVQSSGSVAPPALGDSPTSDLAPVVGANLRRLRV
                     RRGLSLERLARASGVSRAMLGQIELGQSAPTINVLWKVARALDVSFSALIGGGEKSAS
                     AVVLPRAQAKVLTSYDGTFCSRALFPFSGERRKVEFYELRLTGRRSEKAEPHAAGTTE
                     NLIVAAGKLELVTAGERFVLNKGDAIFFNADVPHEYHNPDDAEAVMYLVMTYA"
     gene            complement(2038..2967)
                     /gene="epsC"
                     /locus_tag="VFQ35_27600"
     CDS             complement(2038..2967)
                     /gene="epsC"
                     /locus_tag="VFQ35_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET9934512.1"
                     /translation="MSGLLGVPSESAWDLDQVVQGLRRSRIARNGVESPQRERHKLPS
                     RAALERVTTGLTSALFPSHFGPTDLTPERIDYFVGDVLGTAFRVLESQVLCELDYASP
                     TTPGNPERASALTRNFAERLPAVHELLETDVRAAFEGDPAARSLDEALFCYPGVTAIL
                     HHRLAHELYRLGLPLLARIISEIAHSATGIDIHPGADIGRSFFIDHGTGVVIGETAVV
                     GDRVRLYQGVTLGAKSFPLDERGVALKGKERHPIVEEDVVIYAGATILGRITIGRGST
                     IGGNVWLTRSVPPGSRVTQAQARSDVYSDGGGI"
     gene            complement(2964..3953)
                     /gene="cysK"
                     /locus_tag="VFQ35_27605"
     CDS             complement(2964..3953)
                     /gene="cysK"
                     /locus_tag="VFQ35_27605"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET9934513.1"
                     /translation="MSQNSHHWFEDNALSIGRTPLVRLNRVTDGARARILAKIEARNP
                     AFSVKCRIGAALVWDAEKRGVLKAGKELIEPTSGNTGIALAFVAAARGYPLTLTMPET
                     MSVERRKLLTAYGAKLVLTEGARGMSGAVARAEEIAASAPERYVLLQQFKNAANPRIH
                     ETTTGPEIWEDTNGEIDIFVSGVGTGGTITGVSRYIKQTKNKSIVSVAVEPSASPVIT
                     QKRAGQPLKPSPHKIQGIGAGFVPDVLDLSLVDQVELVSNEEAIDYARRLTREEGILA
                     GISCGAATAVAVRLAKRPENEGKTIVTVLPDSGERYLSTVLFEGVFDAHGLAP"
     gene            complement(4114..5202)
                     /locus_tag="VFQ35_27610"
     CDS             complement(4114..5202)
                     /locus_tag="VFQ35_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HET9934514.1"
                     /translation="MSSSPPRDSAVSVRVEHATKRFGSATQAAAVNDVSFEAPPGAIT
                     ALLGPSGSGKSTLLRMIAGLERPDAGRVFIGDKDVTHVSPRERKVGFVFQSYALFRHL
                     TVFENVAFGLKLRKASRREIDARVRELLGLVQLEGYEKRFPSQLSGGQRQRVALARAL
                     ATDPDVLLLDEPFGALDAKVRVELRRWMLELQDKTHTTTLLVTHDQEEAFELAQHVVL
                     LSEGSLAQAGSPHDLYDGPVTPFVASFIGGASVLSGRVRSGQAWLGELPIRAPHGAPD
                     GAPVRAYVRSGDVRIGRARDAEAPSARVGRLVRLGSKVQVSLLLPSGEALNLEIPRSE
                     LLELGIEAGDPVSVDLRDAKVFVGDYSI"
     gene            complement(5199..6134)
                     /gene="cysW"
                     /locus_tag="VFQ35_27615"
     CDS             complement(5199..6134)
                     /gene="cysW"
                     /locus_tag="VFQ35_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HET9934515.1"
                     /translation="MSGSIGLRTSAEKPLPGSTEPAWVRAGLIAVALLFLGAFVIAPL
                     ALVFLSAFSKGAGPYWQALTDSETFSAIELTLLTAAIAVPANTIFGIAAAWCVAKYEF
                     WGKSALVTLVDLPFSVSPVISGLVFVLLFGSQGIFGASLREHEIKIIFAVPGIVLATT
                     FVTFPLVMREVLAVMQAQGSDEEEAALTLGASGFQIFWKVTLPKIRWGLFYGVILCNA
                     RAMGEFGAVSVVSGHVRGETNTMPLQVEILYNEYNFTAAFAVASLLAALALVTLAVKK
                     AVEWSHGVQERRRESESERVLASQASALRTLQELS"
     gene            complement(6131..6952)
                     /gene="cysT"
                     /locus_tag="VFQ35_27620"
     CDS             complement(6131..6952)
                     /gene="cysT"
                     /locus_tag="VFQ35_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET9934516.1"
                     /translation="MSRRRGVLPGFGLSLGYTLFYLSLVVLVPLGALFFRTSSLGLSD
                     IVRTVTEPRALAAYRLSFGASLAAGLVNAVFGVLLAWVLVRYRFPGRSLVDALIDLPF
                     ALPTAVAGIALTSIYSVNGWFGAAFERAGVRVAFTKLGVTVALIFVGIPFAVRTVQPV
                     LEEFDRELEEAAAVLGASRLQTFSRVILPALLPAVLTGFALAFARALGEYGSVVFISG
                     NMPHKTEIVPLLIVTKLEQYDYAGATALASVMLIASFGLLLTVNLLQRYARRAEA"
     gene            complement(6955..>7990)
                     /locus_tag="VFQ35_27625"
     CDS             complement(6955..>7990)
                     /locus_tag="VFQ35_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9934517.1"
                     /translation="ALFGCNRSNAAPAPVSSSVSSATAGKEPLKAASLLNVSYDPTRE
                     LYGDVNAAFTKKWKAEHGQELTIKQSHGGSGKQARAVIDGLEADVVTLALAYDINAIA
                     SKGKLLPEDWQARLPEQSTPYTSTIVFLVRSGNPKKILDFPDLVKPGVQVITPNPKTS
                     GGARWNYLAAWGHALKKNGNDEKKARDFVQKLYKNVPVLDSGARGSTTTFVERGIGDV
                     LIAWENEALLAVKELGAGKVELVVPPLSILAEPPVALVDKNVDRHGTREIAEAYLKYL
                     YTDEAQELVAKNYYRPRNADVAKRYAARFKSVELFQLSELFGDWNKVQKTHFDDGGVF
                     DQIYQGGSGK"
BASE COUNT         1454 a         2635 c         2551 g         1350 t
ORIGIN      
        1 ccgaccgcgc caggtttcgc gccgccggtg gcctcatcgc ccagcgcgcc gagcttcccg
       61 acttcagccg caacgccggt ccgggccgcg agcgacgtgc cgtacggcgg cgagttgtcg
      121 ctgcccgaac atcagacgcc cgacttgcgg cgcgctcgca ggttgcagag tgggattttc
      181 gacccgtacg cggtgcggcg tgactttccg attctcgagg agcgcgtcaa cggccatcgg
      241 ctcgtgtggc tcgacaacgc cgccacgact cagaagccac gcgccgtgat cgaccgcctc
      301 agctacttct acgtacacga gaactccaac atccatcgcg gcgcgcacac actcgcggca
      361 cgcgcaacgg acgcctacga agccgcgcgt gagtcggtgc gaaggttcct gaacgcgccg
      421 tcgcgggatg gcgtcgtctt cgtgcgcggc gcaacggagg ccatcaacct cgtcgctcaa
      481 gcttggggcc gtcgccacgt cagcgccggc gacgagatcg tcgtcacttg gctcgagcac
      541 cacgccaaca tcgtcccatg gcagcagctc tgcgccgaga aaggagctcg cctgcgagtc
      601 gctcccgtcg acgaccacgg gcaaattcgg ctcgacgagt tcgagaagct actcggcccg
      661 cgtacccggt tggtctctct gtcgcaagtt tcgaacgcgc tcgggaccat cacgccagcg
      721 cgagaaatgg tgcaaatcgc gcaccgctac ggcgcaaaag tgctggtcga cggcgctcag
      781 gccgtctctc acatgccggt cgacgtgcag gccctcgact gcgatttcta cgtcttctcg
      841 gggcacaagg tgttcgcgcc gacgggcatc ggagctctct tcggcaaagc cgacgtcctc
      901 gagagcatgc cgccgtggca aggaggcggg aacatgatcg ccgacgtgac gttcgagcgc
      961 acggtgtatc aagctccgcc gttcaagttc gaggcgggca cggggaacat cgcggacgcg
     1021 gtcggcctcg gcgctgcgct cgactacgtg agcgcgctcg gcatggagac catctcgcgc
     1081 tacgagcacg agctcttggt ttatggaacc gagcgactga aagaggtccc cgggcttcac
     1141 atggttggaa cggcgccgga caaggccgga gtgctctcgt tcgtgttgga tggccaacgc
     1201 accgaagacg tcggcggcgc gctgaaccaa gaaggaattg cggtgcgctc gggccaccac
     1261 tgcgctcagc cgattctgag gcgcttcggg ctcgaagcga gcgtgcgagc gtcgctcgcc
     1321 atctacaaca cctgtgagga catcgacgcg ctgaccgatg cgctcaagcg cattcaagcg
     1381 agccggccgt cttagagctt caggcgtacg tcatcacgag atacatcacg gcctcggcgt
     1441 catccgggtt gtggtactcg tgcggaacgt cggcgttgaa gaatatcgcg tcgcccttgt
     1501 tcaaaacgaa gcgctcaccg gcggtcacga gctcgagctt ccccgccgcg acgatcaggt
     1561 tctcggtcgt cccggcggcg tgcggctctg ccttttcgga gcgtcgcccg gtcaagcgga
     1621 gctcgtagaa ttccactttt cggcgctcac cgctgaacgg aaaaagcgcc cgcgaacaga
     1681 acgtgccgtc gtaagacgtg agcacctttg cttgcgcgcg cggcaagacg acggcagaag
     1741 cgctcttctc cccgcccccg atcagcgccg aaaacgaaac gtcgagcgcg cgcgccactt
     1801 tccacagcac gttgatcgtc ggcgcgctct gcccgagctc gatttgtccc aacatggcgc
     1861 ggctcactcc cgacgcccgc gctagacgct cgagcgagag cccgcgccgg acccgcagcc
     1921 gacgcaggtt cgcgccgacc acgggtgcca ggtccgacgt cggcgagtca ccgagtgcag
     1981 gcggcgccac cgaaccgctc gactggacgt cacagtcgga tactcgacgc gcttcgctca
     2041 tatgcctccc ccgtccgaat agacgtcgct gcgagcctgc gcctgggtca cgcgactgcc
     2101 cggcggaacg ctgcgcgtga gccatacgtt cccgccgatg gtcgagccgc gcccgatcgt
     2161 gatccggccg agaatggtcg cgcccgcgta gatcactacg tcctcttcaa cgatggggtg
     2221 gcgctccttg cctttgagtg cgacgccgcg ctcgtcgagc ggaaagctct tcgcacccag
     2281 agtgacgcct tgatagaggc gcacacgatc tccgacgacc gccgtttctc caatcacgac
     2341 cccagtgcca tggtcgatga agaagctcct gccaatgtcg gcgccggggt gaatgtcgat
     2401 accggtcgcg ctgtgcgcga tttcggagat gatgcgcgcg aggagcggca acccgaggcg
     2461 ataaagctcg tgagccagcc ggtggtgcaa aatcgcggtg acccccggat agcagaacag
     2521 cgcttcgtcg aggcttcgag ccgcaggatc gccttcgaag gcggcgcgca cgtcggtctc
     2581 gagcagttcg tgcaccgccg gtagcctttc cgcaaaattt cgagtcaggg cgctggctcg
     2641 ctccgggttg ccgggggtcg tcgggcttgc gtaatcgagc tcgcacagga cctggctctc
     2701 cagcacgcga aacgcagtcc cgagcacgtc gccgacgaag tagtcgatgc gctcaggagt
     2761 caaatcggtg ggaccgaagt gggaaggaaa cagggccgag gtgagccccg tcgtcacccg
     2821 ttccagcgcc gcgcgagatg gcagcttgtg acgctctcgc tgcggggatt cgacgccatt
     2881 gcgagcaatt cgcgagcgac gaaggccctg aacgacctgg tccaagtccc aagcgctctc
     2941 gctcggcaca ccgagcaggc cactcatggc gcaagtccgt gtgcgtcgaa gaccccttcg
     3001 aacaagaccg tgctgaggta gcgctccccg gagtcgggca gcaccgttac gatggtcttg
     3061 ccttcgttct cggggcgctt cgcgaggcgc accgcaactg ccgtcgcggc cccacacgag
     3121 atgcctgcaa ggatcccttc ctcacgcgtg agtcggcgcg catagtcgat ggcctcttcg
     3181 ttcgaaacga gctcgacctg gtcgacgagc gacaagtcga gtacgtccgg cacgaagccc
     3241 gctccgatgc cctggatctt gtgtggggac ggcttcagcg gctgccctgc tcgcttctgc
     3301 gtgatcaccg gactcgcgct cggctcgacc gcgaccgata caatcgactt gtttttggtc
     3361 tgcttgatgt accgagagac gccggtgatg gtgccgccgg tgccaacccc cgagacgaag
     3421 atgtcgatct ccccgttcgt gtcttcccaa atttcggggc cggtagtcgt ctcgtgaatg
     3481 cgggggttcg ccgcgttctt gaactgttgc aagagcacgt agcgctcggg cgccgatgcg
     3541 gcgatctctt cggccctggc cacggcaccg ctcatgccgc gcgcaccttc ggtcaggacc
     3601 agcttcgctc cgtacgcggt cagcaacttg cgtcgctcga cgctcatggt ctcgggcatc
     3661 gtcagcgtca gcggatatcc gcgcgcggcc gccacgaacg cgagcgcgat gcccgtgttt
     3721 ccgctcgtgg gctcgatgag ctcctttcca gccttgagca cgccgcgctt ttcggcgtcc
     3781 cagaccaacg ccgcgccgat gcggcacttc accgagaaag cgggattgcg cgcttcgatc
     3841 ttggccagaa ttcgggcgcg tgcgccgtcg gtcacgcggt tcagtcgaac gagcggcgtc
     3901 cgaccgatcg agagagcgtt gtcttcgaac cagtgatgag aattttgaga catggggcac
     3961 ctccgttggc gcttgagcga agtgacgaac gccatgttcg gctccgacca tgctggcaac
     4021 gggctgagta tgaagcgccg gcgtgccgct agcgacgcgc cggctcgata cagtgccgaa
     4081 acaggtatat ttcccgcatg ggagggacct cactcagatc gaatagtcac cgacgaagac
     4141 cttcgcgtct cgcaaatcga cgctgaccgg atcccccgct tcgatgccga gctcgaggag
     4201 ctcgctgcgc ggaatctcca agttcagcgc ttcgccgctc gggaggagga gcgacacctg
     4261 aaccttcgag cccagtcgca ccaggcgccc gacgcgcgcg gaaggagcct cggcgtcgcg
     4321 cgcacggccg atgcgaacgt cgccggagcg cacgtaagca cgcaccgggg cgccgtccgg
     4381 cgcgccgtga ggtgcacgga tcggcaattc gccgagccag gcctgcccgc ttcgcactct
     4441 gcccgagagg acgctggcgc cgccgatgaa cgaagccacg aacggagtca cgggcccgtc
     4501 gtaaagatca tgagggctcc ccgcttgagc gaggctccct tcggagagga ggacgacgtg
     4561 ttgcgcgagc tcgaacgcct cttcctggtc gtgagtcacg agcagggtcg tcgtgtgcgt
     4621 cttgtcttgg agctccagca tccagcgccg cagctcgacg cgcaccttgg catcgagagc
     4681 gccgaacggt tcgtcgagga gcagcacgtc cggatccgtt gcgagggcgc gagcgagcgc
     4741 gacgcgctgg cgctgcccac ccgagagctg cgagggaaag cgtttttcgt atccctcgag
     4801 ctgaaccagc ccgagcaatt ctctaacgcg cgcgtcgatt tcgcggcgag acgcctttcg
     4861 cagcttgagc ccgaacgcca cgttttcgaa cacggtgagg tgacgaaaga gcgcgtagct
     4921 ctggaacacg aagccgactt tgcgttcgcg cggcgagacg tgcgtaacgt ccttgtcccc
     4981 gatgaagacg cgacctgcgt cgggacgctc gagtccggcg atcatccgaa gcagcgtcga
     5041 cttgccagag ccagacggcc cgagcagagc ggtgatcgcg ccgggcggcg cctcgaagga
     5101 cacgtcgttg acggccgcgg cttgcgtcgc gctaccgaag cgcttcgtcg cgtgctccac
     5161 gcgcacgctc actgcactgt ctcgcggtgg actgctgctc atgatagctc ctgaagggtg
     5221 cgcaacgcgg acgcttggct cgcgaggaca cgctcgcttt cgctctcgcg gcgccgctct
     5281 tgcacgccat gtgaccactc gaccgccttc ttgacggcca aggtcacgag cgcgagcgct
     5341 gccagcaacg aagccaccgc gaacgcggcc gtgaagttgt actcgttgta aagaatttcg
     5401 acctgcagcg gcatcgtgtt cgtttcgccg cgcacgtgtc ccgagacgac cgaaaccgcg
     5461 ccgaactcgc ccatcgcgcg cgcgttgcac aagatcacgc cgtagaagag cccccagcgg
     5521 atcttgggga gagtcacctt ccagaaaatt tgaaagccgc tcgcgccgag ggtcagcgct
     5581 gcttcttctt cgtcgctgcc ttgtgcctgc atgaccgcga gcacctcacg catcacgagc
     5641 ggaaacgtga cgaaggtggt cgcgagcacg atgccgggca ccgcgaagat gatcttgatc
     5701 tcgtgctcgc gaaggctcgc cccgaagatg ccttgcgagc cgaagagcag cacgaacacc
     5761 aaccccgaga tcacggggga cacgctgaag ggcagatcca cgagcgtgac gagcgcgctc
     5821 ttgccccaga actcgtactt cgcgacgcac cacgccgcgg cgattccgaa gatggtgttc
     5881 gcaggaaccg cgatggcggc cgtgagcagc gtgagctcga tcgccgaaaa ggtttccgag
     5941 tccgtcagcg cctgccaata gggcccggcc cccttggaga aggcggaaag aaataccaac
     6001 gcgagcggag cgatcacgaa agcgcccagg aacaggagcg caactgcgat gagcccggcg
     6061 cgcacccacg ccggttcggt gctgccaggc agcggcttct ccgcgctcgt ccgaagcccg
     6121 atgctgccgc tcatgcttct gccctccgcg catagcgttg cagcaggttc acggtgagca
     6181 ggagcccgaa gctcgcgatg agcatgaccg acgcgagcgc ggtcgccccc gcgtagtcgt
     6241 actgttcgag cttggtgacg atgagcagcg gaacgatctc ggtcttgtgc ggcatgttgc
     6301 ccgagatgaa gacgacgctg ccgtactcgc cgagcgcgcg cgcaaacgcc aatgcgaaac
     6361 cggtcaacac cgccggcagc agcgccggca agatcacccg cgagaacgtc tgaaggcgcg
     6421 acgcccccag cacggcggcg gcttcttcga gctctcggtc gaactcttcg agcaccggtt
     6481 gcaccgttcg caccgcgaac ggaataccga cgaatatcaa cgcgaccgtg accccgagct
     6541 tcgtgaaggc cacgcgcacg cccgcgcgct cgaaggcggc accgaaccaa ccgttcacgg
     6601 agtaaatact cgtgagcgca atgccagcga cggcggtggg caaagcgaag ggcaaatcaa
     6661 tcagcgcgtc gacgagcgag cgcccgggga accgatagcg cacgagcacc cacgcaagca
     6721 acactccaaa caccgcgttg acgagccccg ccgcgagaga cgcgccgaag ctcaagcggt
     6781 atgcggcgag cgcgcgcggc tcggtgacgg tgcgcacgat atcgctgagc ccgaggctcg
     6841 aggtgcgaaa aaacagggcc ccgagcggca ccagcacgac gaggctcaga tagaagagcg
     6901 tgtagccgag gctcagccca aaacccggta gcaccccacg tcgccggctc atggctactt
     6961 ccctgagccg ccctggtaga tttgatcgaa cacgccgcca tcgtcgaagt gcgtcttctg
     7021 aaccttgttc cagtctccga agagctcgct caactggaat agctcgaccg acttgaagcg
     7081 cgctgcgtag cgcttcgcga cgtccgcgtt gcgcggccga tagtagttct tcgcgacgag
     7141 ctcttgggcc tcgtcggtgt acaggtactt gaggtacgcc tctgcgatct cgcgcgtgcc
     7201 gtgacggtcg acgttcttgt cgacgagcgc gaccggaggt tccgccaaga tgctgagagg
     7261 cgggaccacg agctccacct taccggcgcc gagctccttc accgcgagca acgcctcgtt
     7321 ctcccaagca atcagcacgt ccccgatgcc gcgctcgacg aaagtcgtcg tggagccgcg
     7381 cgcacccgag tcgaggacgg gcacgttctt gtagagcttt tggacgaagt cgcgcgcctt
     7441 cttttcgtcg ttgccgttct tcttgagagc gtggccccaa gccgcgaggt agttccagcg
     7501 cgcgccaccc gaggtcttgg gattgggggt gatgacttgc acgccgggct tcacgaggtc
     7561 gggaaaatcg aggatctttt tcggatttcc ggaacgcacc aggaacacga tcgtcgaggt
     7621 atagggcgtg ctctgctcgg gtaagcgcgc ctgccagtcc tccgggagga gcttgccctt
     7681 gctcgcaatc gcgttgatgt cgtacgcgag cgcgagcgta acgacgtccg cttcgagccc
     7741 gtcgatgacg gcgcgtgctt gcttgccaga tccgccgtgc gattgcttga tggtcagctc
     7801 ctggccgtgc tccgccttcc acttcttcgt gaaagcagcg ttcacgtcgc cgtagagctc
     7861 gcgagtcgga tcgtaagaaa cgttgagcag cgaggctgcc ttgagcggtt cctttcccgc
     7921 cgtcgcgctc gagaccgagg agcttacggg agcaggagct gcgttggagc gattgcagcc
     7981 gaacaaagct
//
LOCUS       DASTKS010001289         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1547900, whole genome shotgun sequence.
ACCESSION   DASTKS010001289 DASTKS010000000
VERSION     DASTKS010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547900"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1882)
                     /gene="nifA"
                     /locus_tag="VFQ35_27630"
     CDS             complement(206..1882)
                     /gene="nifA"
                     /locus_tag="VFQ35_27630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HET9934518.1"
                     /translation="MNTALSQVDSDENERESNARRAEVQLYGVYEISKLLASPVRLEV
                     TLARVAQLLASFLDMRHGLIALLSESGMPEVVVGSGWSEATAREYFEHLPERAIGRIV
                     ATDMPLVIRSIRDDPLWENWDFGSEQTGDISFVGVPIRDNGKVIGTLTIDREVRSEAS
                     IELDQDVRFLTMVANLVGQTIRLQRLVAIDRERLMEERRRLERELEQAAPERRESVLA
                     GIVGESDALQSVIEKVRVVARSNSTVLLRGESGTGKELFARAIHDLSSRSGNAFVKIN
                     CAALPESILESELFGHEKGAFTGAAAMRKGRFELANGGTLFLDEIGEISPAFQAKLLR
                     VLQEGEFERVGGTSTLKVDVRLVAATNRNLEEAVACGEFRADLYYRISVIPIFLPPLR
                     DRSTDIPILAKEFLRRYNSEHGAALAFAKDGIAVLSACLFPGNVRELENCVRRTATLA
                     ASSSIHATDFACNKHECLSAALWQNARASGLSGFVPLPLSKGPRPSASSLDLGELDGQ
                     DEDVGTERQRLVEAMESAGWVQAKAARLLGLTPRQIGYALRKYDIPIKKF"
     gene            2502..2762
                     /locus_tag="VFQ35_27635"
     CDS             2502..2762
                     /locus_tag="VFQ35_27635"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HET9934519.1"
                     /translation="MISPDEVRTLLARAFPDAEIDVVDLTGTQDHYQARIVSTAFEGR
                     TPIEQHQLVYGALGEAMDGPIHALALKTYTPRGWSQLNGGST"
     gene            2759..3073
                     /gene="grxD"
                     /locus_tag="VFQ35_27640"
     CDS             2759..3073
                     /gene="grxD"
                     /locus_tag="VFQ35_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET9934520.1"
                     /translation="MNTQLEERILSTLRSKRVVLFMKGKKALPQCGFSAAVVSILNRR
                     GVDFLDVDVLSDAELRQGLKEFSKWPTFPQLYVDGKLIGGSDIVAGMDQSGELERVLG
                     TA"
     gene            3107..3574
                     /locus_tag="VFQ35_27645"
     CDS             3107..3574
                     /locus_tag="VFQ35_27645"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934521.1"
                     /translation="MMRKEQHNVTSSVTHPGLPPHALQAPSPFLYERIGGRPGIRRLV
                     TAFYRRVALDPGAADLRAMHTDIAGARMRLERFLVEWLGGPKAPVNARKPTGLRARHF
                     SFRVGHAEKSIWMRCMSEALDETVQDPKLKSELEAAFEQTARGIVNRDTWNGT"
BASE COUNT          708 a         1221 c         1145 g          685 t
ORIGIN      
        1 cgcaactccg cgcggagccc ccggctacct gagcccgagt gaggcccgcc gagcgcaact
       61 ctcatcctcg agaagcgctc gagcgggccc gcgcaacgca ccgaaggtaa cgcccgccat
      121 cgcatggcct tcgagcggcc acgcgcgacg caccgagtgt gacgctcgcc atcgcacggc
      181 gtttcgtgcc tcggcggcgc ggcgctcaga acttcttgat cgggatgtcg tacttccgaa
      241 gagcgtagcc aatttggcgg ggggtcaggc ccaacaggcg ggccgccttg gcttgcaccc
      301 agcccgcgga ttccatcgcc tcgaccaacc gttgtcgctc tgttccgacg tcttcgtctt
      361 ggccatcgag ctcgccgaga tccaaagaag atgcagaagg acgagggccc ttgctcaagg
      421 gcagcggaac gaacccgctg agcccagacg cccttgcgtt ctgccacaac gccgccgaca
      481 agcattcgtg cttgttgcac gcgaaatcgg tagcgtggat cgacgagctc gcggcgagcg
      541 tagcggtgcg ccgcacacag ttctccagct ctcgcacgtt accggggaag agacacgccg
      601 agagcaccgc gatcccgtct ttggcgaacg cgagcgcggc accgtgctcg ctgttgtaac
      661 ggcgtaggaa ctccttcgcc aagatgggga tgtcggtcga ccgatctcga agcgggggca
      721 agaagatcgg gatcacgctg atgcggtagt agagatcggc gcggaactca ccgcacgcga
      781 ccgcctcctc caagttgcgg ttggtcgcgg ccacgaggcg cacatcgact ttgagcgtcg
      841 acgtcccgcc gacgcgctcg aactctccct cctgcagcac gcgcaagagc ttggcctgaa
      901 aagcaggaga tatctcgccg atttcgtcga gaaagagggt gccaccgttg gctagctcga
      961 agcgaccttt gcgcatcgcg gccgcacccg tgaatgcgcc cttctcgtgc ccgaacagct
     1021 cggattcgag gatgctctcg ggcagggccg cgcagttgat cttgacgaag gcgtttccgc
     1081 tccgggacga caggtcatgg atcgcgcggg cgaagagctc tttgccggtg cccgactcgc
     1141 cccgcaacaa gacggtggag ttgctgcgcg cgacgacgcg caccttctct atcaccgact
     1201 gcagagcgtc gctctcaccg acgatgcccg ccagcacact ctcacgacgc tccggagcgg
     1261 cttgctccaa ctcgcgctcg agccggcgcc gttcctccat gagccgttcg cgatcgatgg
     1321 ccacgagtcg ttgcagccga atcgtctggc ctaccaggtt tgccaccatc gtgaggaagc
     1381 gcacgtcctg gtcgagctcg atggaagctt cggagcgcac ctcgcgatcg atcgtcaacg
     1441 tgccgatgac cttcccgttg tctcgtatgg gcactccgac gaacgagatg tctcccgtct
     1501 gttcgctgcc gaagtcccaa ttctcccaca gcgggtcgtc ccgaatgctg cggatgacga
     1561 gtggcatgtc ggtcgccacg atgcggccga tcgctcgttc ggggagatgt tcgaaatatt
     1621 cgcgcgcagt cgcctcgctc cagcctgagc ctaccaccac ctcgggcatt ccggactccg
     1681 agagcagcgc aatgagcccg tgccgcatat cgagaaaact cgccaagagt tgggctactc
     1741 gcgccagggt cacctccaac cgtacgggtg acgctagcag cttggaaatc tcgtagacgc
     1801 cgtagagttg gacctcagca cggcgcgcgt tgctctcacg ctcgttctcg tcgctgtcga
     1861 cctgtgagag ggctgtgttc atttgaaagc ctcggccatg ggatcaaaac gtgagctggt
     1921 tgtcgccgat cgaatacatc gcccagtgtt gcgggattca gtcgacgcac gtttcgcaga
     1981 catactggtc acgaatcggc gcgcttctcg gctcgatcag cgtcagatgg cgcgccgcca
     2041 acgaagacac gaaacacgtc tgcttcgttt cagtcgcttc gtctaaaggt cgctctgaac
     2101 cgcgattcgt aagtaccgcg cggtaggagt ccacatagca aacattgcgc cagcgcacac
     2161 attttcgtcg tcgactccga gcggcgcgca ttgttttcgt tcagttttct caaaaggatc
     2221 tgacgagtgt cattcgcagg tcggatcgag ccgagcgcgt gcctgcaatc gtcgcgacac
     2281 gaaatgcgcg gtgcctcgta acgagacgtc acccacccgt gcaaccgcgt accaagcgcg
     2341 acgttggcaa cggggattgt ttgaatggag tgcgacaacg aacctgtcgt gaaagcgaca
     2401 gaaggccttt ccaacccggg cgattgacgc gaggtaccgg cggttcacgt cgcgcgaaga
     2461 ttggcacgtc gagtgctttt gcgcgctctc gtaggccctg catgatctct cctgatgaag
     2521 ttcggacgct gctcgctcgt gcgtttccag acgccgagat cgacgtcgtc gatttgaccg
     2581 gaacccaaga tcactaccaa gcgcgcatcg tcagcaccgc tttcgaaggc agaacaccga
     2641 tcgagcagca tcagctcgta tacggcgcgc tgggcgaagc gatggacggc cccatccacg
     2701 cgctcgcgct caagacctac acgccacgag gctggtccca gctcaacgga ggttcgacgt
     2761 gaacacgcaa ctcgaagaac gcattttgtc gacgcttcgc tcgaagcgag tcgttttgtt
     2821 catgaagggg aagaaggcgc tcccgcagtg cggcttctcc gcggcggtgg tctccatcct
     2881 gaaccggcgc ggggtcgact tcctcgacgt cgatgtgctg agcgacgccg agctccgcca
     2941 aggattgaaa gagttctcga agtggcccac gtttccgcag ctttacgtcg atggcaagct
     3001 cattggcggc agcgacatcg tggccggcat ggaccagagc ggtgagctcg agcgcgtgct
     3061 cgggacagcc tagccgaggc tcgcgcctgg ttccgagaac tcatcgatga tgaggaagga
     3121 gcaacacaac gtgaccagca gcgttacgca cccgggctta ccgcctcatg cgttgcaggc
     3181 cccctcaccg ttcctctacg aacgcatcgg tggccgtccg ggcatccgac ggttggtcac
     3241 cgcgttttat cgacgcgtcg cgctcgatcc cggggccgcc gacctccgcg cgatgcacac
     3301 cgacatcgcc ggcgctcgaa tgcgcctcga gcgcttcctc gtcgaatggc tcggtgggcc
     3361 caaggctccc gtcaatgcgc gcaagcccac cggcctccgc gcgcggcatt tttcgtttcg
     3421 agtcgggcac gccgagaaat ccatttggat gcgctgcatg agcgaggccc tcgacgaaac
     3481 cgtccaagac ccgaagctga agagcgagct cgaggcagcc ttcgagcaaa ccgcgcgggg
     3541 aatcgtcaat cgcgacacct ggaacggcac ctgaggtttt aagatgcccc tttacgactt
     3601 tcgctgcccg gcttgcgctc acgtcttcga agagctcgtg cgtcacgggg agatcccgga
     3661 gtgcccccgt tgcggcaacg cgagcccaga gaagcaaatc agcgcgccgt cggctcccgg
     3721 ccgaagcggt gaggtcgtcg cctcggtgcg acggcaagc
//
LOCUS       DASTKS010001290         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_207305, whole genome shotgun sequence.
ACCESSION   DASTKS010001290 DASTKS010000000
VERSION     DASTKS010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207305"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFQ35_27650"
     CDS             complement(<1..1220)
                     /locus_tag="VFQ35_27650"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9934522.1"
                     /translation="MNRILYTLWVVAGLAATAPVRAESAPSDPKGTPGAGKLTKAPRL
                     LHFAPPELPPGALVENPISIGLKLSIAADGSVSAAELVEPGDPVFDEAARAAALKLKF
                     EPAEIDGVPAAVRISYRYEIKPSAPKPSINDKARFGGVVRDQYGKPLAGVRVELDTGE
                     SGISGDDGRFSIADVPPGNHPVTLSGPTFTPIGTEETFVADKAYEASYEVQVTAVAVP
                     GEERADFEIVVVAARLGSKVAATEVSSEQGARVAGTGGDVVKVVENLPGVARSTVGSG
                     QLVVWGASGQDTRVYVDGVHVPVLYHEGGYRSVVHSDLVRSVELQPGGYGASYGRGIG
                     GLVTVGLKPLDEPGIHGSVQLDAIDAGASLRARLSDKWNISLAGRRSHLDWVLSNVTS
                     EDVGEFVPIPQYWDG"
     gene            complement(1309..2001)
                     /locus_tag="VFQ35_27655"
     CDS             complement(1309..2001)
                     /locus_tag="VFQ35_27655"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9934523.1"
                     /translation="MARRKARSTVYIVSVGAHLALGLAIAMIPKDKLREVVGIALAEN
                     KKPAPKAEPPPKREETPRARPARAAGAQRSQQTAAPTPATAEAAAPPVFTDIGIALDS
                     SAIGGIPMQIAPRSVERVAPVEALPAKPKVLVARVSQDVCSEDLKKARLIRQVQPKYS
                     NTASLANVRGVIRLELDIDERGVVQRVHVVRGLGYGLDESAVEAAKKIEFSPATLCGK
                     PVSSKFTLAFRY"
     gene            complement(2012..2425)
                     /locus_tag="VFQ35_27660"
     CDS             complement(2012..2425)
                     /locus_tag="VFQ35_27660"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9934524.1"
                     /translation="MAGTTAARGGRRAPIVGINVTPMVDVVLVLLVIMMVSAVYIVSQ
                     SLKVELPKTANSDEPAQSPLAVTITKEGKYFFEQKPIDEPGLVRKLKQAKGEKKEPSL
                     VVTADKEAYHGDVVHVIDLAKSEGITKFAINVERD"
     gene            complement(2429..3127)
                     /locus_tag="VFQ35_27665"
     CDS             complement(2429..3127)
                     /locus_tag="VFQ35_27665"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9934525.1"
                     /translation="MVVEKLLKVALLGASWVMYLMIALSVFSIGAMIERWWFFSRRNV
                     DADDLEEGLMKHLANGDREGATQLLERSKAKSFEAEAIAPALRYLDAGADAFIDCVDG
                     ELIKVRQDLERGSNLLGTLGNNAPFIGLLGTVIGVIIAFHQLGTSQAATSMNAVMGGI
                     AEALVSTGVGLFVALPAVVAYNIIQKKIGDIESNATAITKQVSAFLKAHPFHKRESEE
                     RLSHRAFAVVEGHG"
     gene            complement(3149..4153)
                     /locus_tag="VFQ35_27670"
     CDS             complement(3149..4153)
                     /locus_tag="VFQ35_27670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934526.1"
                     /translation="MNLAIVLSHGRLPFAFAVLGALVGCSQGQNSVIGPSEPFRMRGN
                     SVQFIRGNLPGTVPTPGETPEASDLPSIAAVDLNSLIVTQGGSKSISGRASANATSIG
                     IAMEGVGAGYWVVPTRSQDPITGELTWGVASANQSGLVADFDLGIESGVRPLHLVAFD
                     ETGRAGIQFIQKLCVRSAVPDNLNSCEPSVTPPEAVFSLSWDVNADLDLQVLTPDGLL
                     VTPKRGSTIDPDADAGSAPGPLGTIDHDSNANCVSDGVRRESLVFQTESPSPGYLFFA
                     NLHDACKQPVVRFNMQVWSATTDEDGGKRLELKFQRGGELLDSQVNPGSGRGLFVGAY
                     "
     gene            complement(4150..>5090)
                     /locus_tag="VFQ35_27675"
     CDS             complement(4150..>5090)
                     /locus_tag="VFQ35_27675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934527.1"
                     /translation="LYRGSNPKDMLPMAKLTGGMFRVPFGYETPEPAGQRWFMERTQL
                     SRALFPTEIDVGARLSGAISVFRYAFAVTNGEPLGERSGFQLQDPNANKDFWINAGAV
                     VQVLPMLRIAGGVSGVTGRGFHAGTSVTKSSVTWRDANDNGVVDPNELIGVAAQAETP
                     SKSFERFAVGGDLQLYVKTPLGETMLYGEMVTAKNLDRGFFVADPVAPPGIDLREFGW
                     YVGILQPVTEYGIVGFRAEYYDPNADSSEQRAAKILPLKNSVRTYSPLVGLQLPKRAR
                     LLFQYDIIRDHLARDAQGVPTDLQNDAWTLRLQVHL"
BASE COUNT          852 a         1604 c         1657 g          977 t
ORIGIN      
        1 ccatcccagt attgcggaat cgggacgaac tcgccgacgt cctcgctggt gacatttgag
       61 agcacccaat cgaggtgact gcgacgtcct gcgagggaga tgttccactt gtccgagagc
      121 ctcgcgcgga ggctcgcgcc ggcgtcgatg gcatcgagct gtacgctgcc gtggatgcca
      181 ggttcatcca atggcttgag ccccaccgtg acgagcccgc cgataccgcg cccgtagctt
      241 gcgccgtagc ctcccggttg tagctccacg ctgcgtacga ggtccgagtg cacgaccgag
      301 cggtaccctc cctcgtggta aaggacgggg acatggacgc cgtcgacgta gacgcgcgtg
      361 tcttgcccgc tcgcgcccca gacgacgagc tgccccgaac cgaccgtcga acgcgcgacg
      421 ccgggaaggt tttcgacgac tttgacgaca tcgccccccg taccggcaac gcgagcccct
      481 tgttccgagc tcacctcggt cgcagcgact ttgctgccga gacgcgcggc cacgaccacg
      541 atttcgaaat cggcgcgctc ttctcccggt acggcgacgg cggtgacttg gacttcgtag
      601 cttgcctcgt acgccttgtc ggcaacgaac gtttcctcgg tgccgatggg cgtaaaggtg
      661 ggccccgaga gcgtcacggg gtggttgccg ggaggtacgt cggcgatcga aaagcgcccg
      721 tcgtcgccgc tgatgccgct ctcgcccgtg tcgagctcga cgcgcactcc cgcgagcggc
      781 ttaccgtatt ggtcgcgcac gacgccgccg aatcgagcct tgtcgttgat gctcggcttg
      841 ggcgcgctcg gcttgatttc gtaacgatag ctgatgcgca cggccgccgg gacgccgtcg
      901 atttcggcgg gttcgaactt gagcttcagt gcggccgcgc gcgccgcctc gtcgaacacg
      961 gggtcgcccg gctcgacgag ctccgctgcg ctcactgaac cgtcggcggc gatgctgagc
     1021 ttgagcccga tgctgattgg gttctctacg agcgcaccgg gcgggagctc gggcggagca
     1081 aagtgaagga ggcgcggggc cttggtcagt ttgccggcgc cgggcgtgcc tttcgggtcg
     1141 ctcggagcgg actctgcgcg gaccggtgcc gtcgcagcca gaccggcaac gacccagagg
     1201 gtatacagta ttcgattcat tcttcggcgc gtgcgttagc tgcgaacgcc agtgagtttg
     1261 ccgagcgcac tcgtgggtag ccgccggcag ctgcaatgtc gatcgtcgtt agtacctgaa
     1321 tgccagtgtg aacttgctcg agacgggctt tccgcaaagg gtcgcgggag aaaactcgat
     1381 cttttttgcc gcttcgaccg ccgactcgtc gagaccgtaa cccaagccgc gcacgacgtg
     1441 gacgcgttgg accacaccgc gttcgtcgat atcgagctcg agacggatga ctccgcgaac
     1501 gttcgcgagc gaagcggtgt tcgaatactt gggctgcact tgtcgaatca gccgcgcctt
     1561 cttgaggtct tcgctgcaga cgtcttgcga gacgcgcgcg acgagcactt tgggctttgc
     1621 gggcagcgcc tcgaccggag cgacccgctc gacgcttctc ggcgcgatct gcatcgggat
     1681 gccgccgatc gccgatgaat cgagcgcgat gccgatgtcc gtgaacacgg gcggcgccgc
     1741 tgcttccgcg gttgccggcg tgggggcggc cgtctgctgc gatcgctgcg cgcccgcggc
     1801 ccgcgccggc cgtgcgcgcg gcgtctcttc gcgcttcggc ggcggctcgg ctttgggggc
     1861 cggctttttg ttttcggcga gggcgatgcc gacgacctcg cgcagcttgt ctttgggaat
     1921 catggcgatc gcgagcccga gcgcgaggtg cgcgccgaca cttacgatgt agacggttga
     1981 gcgggctttg cggcgagcca tggtcggtcc ttcaatcgcg ttccacgttg atagcgaatt
     2041 tcgtgatgcc ttcggatttg gcgaggtcga tcacgtggac gacgtcaccg tggtatgcct
     2101 ctttgtccgc ggtcacgacc aagcttggct ccttcttctc gcctttggcc tgcttcagct
     2161 ttcgaacgag gccaggctcg tcgatcggct tttgctcgaa gaagtacttg ccttccttgg
     2221 tgatcgtcac ggcaaggggc gactgcgcgg gctcgtccga gttcgcggtc ttggggagct
     2281 ccaccttcag gctttgtgaa acgatgtaca cggccgagac catcatgatg acgaggagca
     2341 cgagcaccac gtcgaccatg ggcgtcacgt tgattccgac gatcggcgcg cgacggccgc
     2401 cgcgcgcagc ggtggtaccg gccatggctc agccgtgacc ttcgacgaca gcgaacgcgc
     2461 ggtgcgagag gcgttcctct gactcgcgct tgtggaatgg gtgcgccttc aagaacgcgc
     2521 tcacctgctt cgtgatggcg gtggcgttgc tctcgatgtc gccgatcttc ttctggatga
     2581 tgttgtacgc aacgacggcg ggaagagcga cgaaaagacc gacaccagtc gagacgagcg
     2641 cttcggcgat gccgcccatg accgcgttca tcgaagtcgc ggcctggctc gttccgagct
     2701 ggtggaacgc gatgattacg ccgatgacgg tgccgagcaa cccgatgaac ggcgcgttgt
     2761 tgccgagcgt accgagcagg ttgcttccgc gctcgaggtc ctgtcgcacc ttgatgagct
     2821 cgccgtccac acagtcgatg aaggcgtctg cccccgcgtc gaggtagcga agcgcaggag
     2881 cgatcgcctc ggcctcgaag gacttcgcct tggaccgctc gagcaactga gtcgcgcctt
     2941 cgcgatcgcc gtttgcgagg tgcttcatca acccctcttc gagatcgtcc gcgtcgacgt
     3001 tgcgacgcga gaagaaccac cagcgctcga tcatggcgcc gatggagaac accgagagag
     3061 caatcatcag gtacatcacc cagcttgcgc cgaggagcgc cactttcagc agtttctcga
     3121 caaccatgtt cagtctcctt ggttttcttt agtacgcgcc cacgaacaat cctcggcccg
     3181 agccggggtt cacttgggaa tcgaggagct cgccccctcg ctgaaacttg agctcgaggc
     3241 gcttgccgcc atcttcatcg gtggtagcgc tccaaacctg catgttgaac cgcacgaccg
     3301 gctgcttgca ggcgtcgtgc aagttcgcga agaataggta gccgggcgac ggcgactcgg
     3361 tttggaacac caagctctcg cggcgcacgc catcgctcac gcagttggcg ttcgaatcgt
     3421 gatcgatggt cccgagcggt cccggcgcgc tgcctgcgtc ggcgtccggg tcgatcgtcg
     3481 agccgcgctt gggtgttacg agcaggccgt cgggggtaag gacctgaagg tcgagatcgg
     3541 cgttcacgtc ccagctgagc gagaagactg cctctggggg ggtgacgctc ggttcgcacg
     3601 agttcaagtt gtcgggaacg gcgcttcgca cacagagctt ttggatgaac tggatgccag
     3661 cgcgaccggt ctcgtcgaag gcgactaggt gcagagggcg cacgccgctt tcgattccga
     3721 gatcgaagtc ggcgacgagc ccactttggt tcgccgaagc cacgccccag gtgagctcgc
     3781 ccgtgattgg atcttgggag cgcgtcggga cgacccagta gcccgccccc actccttcca
     3841 tcgcgatgcc gatcgacgtc gcgttggcgc tcgctcgacc cgaaatgctc ttcgagccgc
     3901 cctgggtgac gatcaagctg ttcaagtcga ccgccgcgat gctgggcagg tcgcttgctt
     3961 cgggtgtctc gccgggtgtg gggacggtcc ccggcaaatt gccgcgaatg aactggacgc
     4021 tgttcccgcg catccggaac ggctcgctcg gaccaattac cgagttttgt ccctggctgc
     4081 agccgacgag cgcgcccagc accgcgaacg cgaagggcag tctcccatga gaaaggacga
     4141 tagcgagatt catagatgca cctgcaaccg cagggtccaa gcgtcgttct ggagatccgt
     4201 cgggacgcct tgcgcgtcgc gcgcgagatg atcgcggatg atgtcgtact gaaagaggag
     4261 tcgtgctcgt ttcggcagct gcaacccgac gagcggtgag tacgttcgca ccgagttctt
     4321 cagcgggagg atcttcgccg cgcgctgctc gctcgagtcg gcgttcggat cataatactc
     4381 ggcgcgaaat ccgacgatgc catattcagt gacgggctga agaattccaa cgtaccagcc
     4441 gaactctcgc agatcgattc ccggaggcgc cacggggtct gccacgaaaa agccacgatc
     4501 aaggttcttc gccgtcacca tttcgccgta aagcatcgtt tcgccgagcg gcgtcttcac
     4561 atagagctgg aggtcgccgc cgaccgcgaa gcgctcgaac gacttcgacg gcgtctccgc
     4621 ttgcgcggca acgccgatga gctcgttcgg atcgacgact ccgttgtcgt tcgcgtcgcg
     4681 ccaggtgacg ctcgacttcg tgaccgacgt gccggcgtga aaccctcgcc cagtcacgcc
     4741 cgaaacgccg cccgctatcc gcagcatggg aagcacctgg accaccgcgc ccgcattgat
     4801 ccaaaagtcc ttgttcgcgt tcgggtcttg tagttgaaag ccgctgcgct cgccgagggg
     4861 ctccccattc gtgacggcga aggcataacg gaagaccgag atcgcgccgg agagtcgcgc
     4921 gccgacgtcg atttcggtgg ggaagagcgc gcgcgacagc tgcgtgcgct ccatgaacca
     4981 gcgttgacct gcgggctctg gagtctcgta gccgaaggga acgcgaaaca tgcccccggt
     5041 cagcttcgcc attgggagca tgtctttggg gttcgacccc cgatagagca
//
LOCUS       DASTKS010001291         8036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1548066, whole genome shotgun sequence.
ACCESSION   DASTKS010001291 DASTKS010000000
VERSION     DASTKS010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548066"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VFQ35_27680"
     CDS             <1..1018
                     /locus_tag="VFQ35_27680"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9934528.1"
                     /translation="YKHIWVFIEYEHGVIHPVSLELLGEGRKLANQLGVELAGVLLGG
                     ERSALLAAANECFERGADVVYLAASQLLADYRNEPYTKVMTELVNKYQPEILLLGATT
                     LGRDLAGAVATTLLTGLTADCTALAIDEERSLAATRPTFGGSLLCTILTLNYRPQMAT
                     VRPRVFSMPERQAGRTGRIVEHPVTLLEESILTKVLEFIPDSRSDKSQLAYADIVVAG
                     GLGLKSPENFRLVRELAETLGGDYGGTRPLVQKGWIPHDRQIGQTGKTIRPKLYIAAG
                     ISGAIQHRVGVEGADLIVAINTDPAAAIFDFAHVGIVADAVDLLPALTAAFRNRFRVN
                     RLAG"
     gene            1031..2335
                     /locus_tag="VFQ35_27685"
     CDS             1031..2335
                     /locus_tag="VFQ35_27685"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9934529.1"
                     /translation="MTQEHFDAIVVGAGPAGSAAAYTMAKAGLNVLQLERGEYPGAKN
                     VQGAILYADALDKLMPDFREDAPLERHVIEQRLWMLDDSSYVGMHYRSEDFNEERPNR
                     YTIIRTQFDKWFARRVREAGALQICETTVTELVRDGNGKVIGVRTDRDGEPLLADVVV
                     LGEGVNGLLGQRAGLRKELKPDSVALAVKEMHFLPQEVIDARFNLKGTEGVVVEAMGT
                     VTAGMVGTGFIYTNKESLSIGIGCLVSDFVESGDTPAALLDRFKNHPAIKPLLEGSEV
                     KEYSGHLIPEGGYKAVPELYGDGWLVVGDAGHFVNGVHREGSNLAMTTGRIAGEVIAE
                     AKAKGTALSSKALGEYKRRLEQTFVMKDLKKYKDLPSFMHKNRHMFEVYPRLLSQATQ
                     TWFRVDGVDKLSKEREIISSFRKSRSLGGLLGDAFKMMRVWR"
     gene            2332..2631
                     /locus_tag="VFQ35_27690"
     CDS             2332..2631
                     /locus_tag="VFQ35_27690"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9934530.1"
                     /translation="MTTTKTNANVEQKLFQNRYQVDGGRPHIVVKPHETPSKYLLAMT
                     TACPAGCYSKNDSGQVEISVDGCVECGTCRIVCATTGEIEWNYPRGGFGVAYKFG"
     gene            2710..3030
                     /locus_tag="VFQ35_27695"
     CDS             2710..3030
                     /locus_tag="VFQ35_27695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9934531.1"
                     /translation="MIEVTETAANAIRNAISGSKEPVEGLRIMVQSGGCSGYKYSMGL
                     VQAAEPGDVVVEQAGVKVFVDEKSVPLLQGVVLDFTVSMDGAGFTFNNPNAQSSCSCG
                     KSFS"
     gene            3041..3973
                     /gene="nifU"
                     /locus_tag="VFQ35_27700"
     CDS             3041..3973
                     /gene="nifU"
                     /locus_tag="VFQ35_27700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HET9934532.1"
                     /translation="MWDYSEKVKEYFFNPKNSGALEEANGVGDVGAIACGDALRLMIK
                     VDPETDIITDARFQTFGCGSAIASSSALTELIIGKTIEEALKITNQDIADYLGGLPPE
                     KMHCSVMGYEALRAAVANYKGEAWVDDHEEGELICKCFGVDEGMVERAVRMNTLTTLE
                     QVTHFTKAVGGCGTCVEKVEAVVARVNAAMVDEGVISADIAFDPARAAAVKKPKKKVS
                     PLAPQKKPAPAKLNTLQRIRLIEESLEELRPVFRRDGGDCELVDVEEDRVFVRFSGAC
                     LGCQLESVTLQGVQERLMERLGQPLRVIPVQQSH"
     gene            4007..5221
                     /gene="nifS"
                     /locus_tag="VFQ35_27705"
     CDS             4007..5221
                     /gene="nifS"
                     /locus_tag="VFQ35_27705"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HET9934533.1"
                     /translation="MKPVYLDNNATTRVDPRVVEAMLPFFTEQFGNASSMHSFGAAVG
                     GALKQARQSLQELIGAEHDHEVVFTSGGTEADNTALLSALETQVGRNEIVTSKVEHPA
                     ILSLCKHLEETRGVKVHYTPVDSRGRLDIDAYRAALGPKTAIASVMWANNETGTLFPV
                     ARLAELAHEAGALFHTDAVQAVGKVPVVVKDTEIDMLSLSGHKLHGPKGIGALYVRRG
                     VRLRPLVRGGHQERGRRAGTENAPAVVGLGKAAELALAHFGDEQGRVRALRDRLERGI
                     LQTIERANLMGDPDDRLPNTTNVAFEFIEGEAILLLLNRAGIAASSGSACTSGSLEPS
                     HVLRAMNVPYTCAHGAIRFSFSRENDDADVDWVLEQLPGVISTLRSISPFWKDDRAAA
                     KQGPQDFKPTYA"
     gene            5238..5570
                     /gene="nifW"
                     /locus_tag="VFQ35_27710"
     CDS             5238..5570
                     /gene="nifW"
                     /locus_tag="VFQ35_27710"
                     /inference="COORDINATES: protein motif:HMM:NF002009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase stabilizing/protective protein NifW"
                     /protein_id="HET9934534.1"
                     /translation="MTVLDDLRRLSAAEEFFSYLGVESDPSVIRVARLHILRRMGDYL
                     RGEQLSGLDDATTRERCRHHLVAAYEDFVKSTPLEQRVFKVLKDAVAPKKRAFVPLAA
                     LSKQENED"
     gene            5571..6425
                     /locus_tag="VFQ35_27715"
     CDS             5571..6425
                     /locus_tag="VFQ35_27715"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9934535.1"
                     /translation="MHSVVCIKQVPDSAQIRVHPVTNTIMRQGVPSIINPYDLFALEE
                     ALRLRDRYGGEVTVLTMGPPMAEDSLRKALTFGADRAVLLTDRFFAGSDTLATTYALA
                     TAIKKVGAEWGNPTLVFTGKQTIDGDTAQVGPGIAKRLGLTQLTYVSEIEDIDLHHAG
                     VIVRRRAEGGVQRLSTRLPALVTVIEGINEIRRGTMTDALRAARTQIVRWSAADAGVE
                     EIAKCGLRGSPTIVKQVYAPSARAEKAELVPTENQSFPQMADALFGRIFEKKPGLEAD
                     LAKFVSER"
     gene            complement(6472..7221)
                     /locus_tag="VFQ35_27720"
     CDS             complement(6472..7221)
                     /locus_tag="VFQ35_27720"
                     /inference="COORDINATES: protein motif:HMM:NF015949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HET9934536.1"
                     /translation="MKQSLYDLLIARAESRSKVNSVLLGLNWSVAQVEAYGLCFSPTN
                     APRTLDWSGTLVGRSAIELAPWIRSLDASTATVGALVVNAVVNHPGNALLESAKPLSS
                     NQPPHLAVFEHFKSAVQGARVVVVGRYPGLERVWSPDQYVCLERRPLPGTVPESNAEQ
                     VLRGADWVFVTASSIVNKTLPSLLRQARGAQIVLMGPSLPWLSELADFGVSYLAGVAV
                     TDGGELMTIAAQAGGTRIFERAVEYRLLRLA"
     gene            7431..7772
                     /locus_tag="VFQ35_27725"
     CDS             7431..7772
                     /locus_tag="VFQ35_27725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9934537.1"
                     /translation="MNIEGVELPVIFRYHAFCCFQQRPPGHPKGSCGAAGGPELWEHL
                     GGAIQGRQLQRVTMTATGCLGFCNAGPLMVVYPEGVWYQPRTKEDIDEIVQSHFVEGR
                     PVARLVVVLER"
     gene            7779..>8036
                     /locus_tag="VFQ35_27730"
     CDS             7779..>8036
                     /locus_tag="VFQ35_27730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934538.1"
                     /translation="MHPAPLEPATETQAESARARVRRYHEGSKHRLDRYAPGPETLDW
                     DGQPSPFRRFAGAARVSLPSSATSEGKLAESLALPLGAAPDE"
BASE COUNT         1545 a         2513 c         2609 g         1369 t
ORIGIN      
        1 ttacaaacac atctgggtct tcatcgagta cgaacacggc gtcattcacc ccgtctcgct
       61 cgagctcctc ggtgaagggc gcaagctcgc gaatcaactc ggggtggagc tcgctggggt
      121 attgctcggc ggggagcgct cggcgttgtt ggccgcagcc aacgagtgct tcgagcgcgg
      181 cgcagacgtc gtgtacctcg cggcgagcca gctgctcgct gactatcgaa atgagccgta
      241 cacgaaggtg atgaccgagc tggtcaacaa gtatcagccg gaaatcctgc tgctcggggc
      301 caccacgttg ggccgcgact tggccggtgc ggtggcgacg acgctgctca cggggctcac
      361 cgccgactgc actgcgctcg cgatcgacga agagcgctcg ctcgcggcga cgcgcccgac
      421 tttcggcggc tcactgctct gcaccattct caccttgaac tatcgcccgc agatggcgac
      481 cgtgcgtccg cgagttttct cgatgccgga gcgccaagcg ggccgcaccg gacgcatcgt
      541 cgaacatccg gtgactctgc tcgaagagag catcctgacc aaggtgctcg agttcatccc
      601 cgacagccgc tccgataagt cacagctcgc gtacgcagac atcgtggtcg cgggtggcct
      661 gggcctgaaa tcaccggaga atttccgctt ggtgcgcgag cttgcggaga cgctgggcgg
      721 cgactacggc ggtacgcgcc cgctggtgca gaaggggtgg atcccacacg accgccaaat
      781 cgggcagacg ggtaagacga ttcgcccgaa gctctacatc gcggccggca tttcgggcgc
      841 gattcagcat cgtgtcggtg tcgaaggcgc ggacctgatc gtcgcgatca atacggaccc
      901 tgccgcggcg atcttcgact tcgcgcacgt cggcatcgtc gcggacgccg tcgacttgct
      961 gccggcgctc accgcagcct ttcgcaaccg cttccgagtg aaccgcctcg ctggttgaag
     1021 gagtacgacc atgacccagg aacatttcga cgcgatcgtg gtgggtgcgg gcccggcggg
     1081 cagcgccgcc gcatacacga tggccaaagc cggcctgaac gtgctgcagc tcgagcgagg
     1141 cgaataccca ggcgcaaaaa acgtccaggg cgccatcctc tacgccgatg cgctcgacaa
     1201 gttgatgcct gacttccgcg aagacgcgcc gctcgagcgg cacgtcatcg aacagcggct
     1261 gtggatgctc gacgatagct cgtacgtcgg catgcactac cggtcggagg acttcaacga
     1321 ggagcgcccg aaccgctaca cgatcattcg cacccaattc gacaagtggt tcgcgcgtcg
     1381 agttcgggaa gcgggcgcac tgcagatctg tgagacgacg gtcaccgagc tcgtgcgcga
     1441 cgggaacgga aaggtcatcg gcgttcgaac ggatcgagac ggagaaccgt tgctcgctga
     1501 cgtcgtcgtc ctcggagaag gcgtgaacgg ccttctgggg cagcgcgcgg ggctgcgcaa
     1561 ggagctgaag cccgacagcg tcgcgctggc cgtgaaagag atgcacttcc tgccgcagga
     1621 ggtcatcgac gcgcgcttca atctgaaggg caccgagggc gtcgtcgtcg aggcgatggg
     1681 cacggtgacc gcggggatgg tcggcacggg gttcatctac accaacaaag agtcgctctc
     1741 gatcggcatc ggttgcttgg tgtccgactt cgtcgagtcc ggtgacaccc cggccgcgct
     1801 tctcgaccgc ttcaagaacc atcctgcgat caagccgctg ctcgagggct ccgaagtgaa
     1861 agagtactcg ggccacttga ttccggaagg cggatacaag gcggttccgg agctctatgg
     1921 agacggctgg ctcgtcgtcg gcgacgcggg ccacttcgtg aacggggtgc accgcgaagg
     1981 ttcgaacctg gcgatgacga ccgggcgcat cgcgggcgag gtcatcgcgg aagcgaaagc
     2041 gaaggggacg gccctctcga gcaaggctct cggtgaatac aagcgccgtc tcgaacagac
     2101 gttcgtgatg aaggacttga agaagtacaa ggaccttccc tccttcatgc acaagaaccg
     2161 acacatgttc gaggtgtatc cgcgcctcct cagccaggcg acgcagacct ggtttcgagt
     2221 cgatggcgtc gacaagctca gcaaggagcg tgagatcatc tcttctttcc gcaagagtcg
     2281 cagcctaggc ggattgctgg gcgacgcttt caagatgatg agggtatggc gatgaccacg
     2341 accaagacga acgcaaacgt ggagcaaaaa ctctttcaga atcgttacca agtcgacggt
     2401 gggcgccccc acatcgtggt gaagccgcac gaaacgccgt cgaagtacct gctcgcgatg
     2461 acgaccgctt gccccgctgg ttgctactcg aagaacgaca gcgggcaggt cgaaatttcc
     2521 gtcgacggct gcgtcgagtg cgggacctgc cgcatcgtgt gcgcgaccac cggcgaaatc
     2581 gagtggaact acccccgtgg tgggtttggc gtcgcctaca agtttggatg aagccagctc
     2641 gaaccacgaa ctcgtctaca cgccgctcag cccgaagcac tgtcgattcg attcagatta
     2701 ggagaaacca tgattgaggt caccgaaacc gccgccaacg cgattcgcaa cgccatctct
     2761 ggatccaagg agcccgtcga aggactccgc atcatggttc agtccggggg gtgctccgga
     2821 tacaagtact cgatgggcct ggtgcaagcc gccgagcccg gtgacgtcgt cgtcgagcaa
     2881 gccggggtca aagtgttcgt cgacgaaaag agcgttccgt tgctgcaggg agtcgtcctc
     2941 gacttcaccg tgagcatgga cggcgcaggt ttcacgttca acaacccgaa cgcccagtcc
     3001 agctgctcct gcggcaagtc cttcagctga gggtcgcacg atgtgggact attcggaaaa
     3061 ggtcaaagag tacttcttca acccgaagaa ctcgggtgcg ctcgaagaag cgaacggcgt
     3121 cggtgacgtc ggcgcgatcg cgtgcggaga cgcgctgcgg ttgatgatca aggtcgatcc
     3181 agagaccgac atcatcaccg acgctcgatt ccaaacgttt ggatgcggct cagcaatcgc
     3241 ctcgtcgtcg gcgctcaccg agctcatcat cggcaagacc atcgaagagg ccctcaagat
     3301 cacgaaccaa gacatcgcgg actacctcgg cggccttccg cccgagaaaa tgcactgctc
     3361 cgtgatgggt tacgaggcgc tccgtgccgc ggtcgcgaac tacaagggtg aggcgtgggt
     3421 cgatgaccac gaggaaggcg agctcatctg caagtgcttc ggcgtcgacg aaggtatggt
     3481 cgaacgcgcg gtgcggatga acacgctgac cacgctcgag caggtgaccc acttcaccaa
     3541 agcggtgggc ggctgcggga cctgcgtgga aaaggtcgaa gcggtggtcg cgcgcgtcaa
     3601 cgccgcgatg gtcgacgagg gcgtcatctc ggccgacatc gcgttcgacc ctgcgcgggc
     3661 cgccgcggtg aagaagccaa aaaagaaggt ttcgccgctt gccccccaaa agaagccggc
     3721 gccggcgaag ctcaacacgc tgcagcgcat tcggctgatc gaggaatcgc tcgaggagct
     3781 ccgaccggtg tttcgtcgcg acgggggcga ctgcgagttg gtcgacgtcg aagaagaccg
     3841 agtcttcgtc cgcttttcgg gtgcgtgcct cggttgccag ctcgaaagcg taacgctcca
     3901 aggcgtccag gagcgcctga tggagcgcct aggacaacct ttgcgcgtca tccctgttca
     3961 acaatcccac tgagaaaccg cggccgcgag aacgagagga aaaacaatga agcccgtata
     4021 tctcgacaac aacgcgacga cgcgggtcga cccccgagtc gtcgaagcga tgctcccgtt
     4081 cttcaccgag caattcggta acgcctcgtc catgcattcg ttcggcgcgg ccgtaggtgg
     4141 agccctcaag caagctcgac agagcctgca ggagttgatc ggcgccgaac acgaccacga
     4201 ggtcgtcttc acgtccggcg gtacggaggc ggacaacacc gctttgctct cggcgctcga
     4261 gacgcaggtt ggtcgcaacg agatcgtgac gagcaaggtc gagcaccctg cgattctatc
     4321 gctctgcaag cacctcgaag agacgcgcgg cgtgaaggtg cactacacgc ccgtcgattc
     4381 gcgcgggcgg ctcgacatcg acgcctatcg cgcggcgctc ggcccgaaga cggcgattgc
     4441 ctcggtgatg tgggcgaaca acgagaccgg taccctgttt ccggtggcgc gcctcgcgga
     4501 gctcgcgcac gaggccggcg cgctgttcca caccgacgcg gtgcaagccg tcggcaaggt
     4561 gccagtcgtc gtcaaagaca cggagatcga catgctctcg ctctctggtc acaagctgca
     4621 cggtccgaag ggaatcggcg cgctctacgt tcgccgcggc gtgcggcttc ggccgctggt
     4681 gcgcggtggg catcaagagc gcggccgtcg cgccggcacc gagaacgcgc cggccgtggt
     4741 cgggctcgga aaggcggccg aactggcgct cgcgcacttc ggtgacgagc aggggcgcgt
     4801 ccgcgcgctg cgtgaccgat tggagcgcgg cattctccaa accatcgagc gcgccaatct
     4861 gatgggcgac cccgacgacc gtttgccgaa cacgacgaac gtcgcgttcg agttcatcga
     4921 gggtgaagcg atcttgctgt tgctcaaccg cgctggcatc gccgcgtcgt ccggctcggc
     4981 gtgcacttcg ggctcactcg agccgtcgca cgtgttgcgc gccatgaacg ttccctatac
     5041 gtgcgctcac ggcgcgattc gcttctcctt ctcgcgggag aacgacgacg ccgacgtcga
     5101 ttgggtgctc gagcagctgc ccggcgtgat cagtacgctg cgctcgatct ctccgttctg
     5161 gaaggacgat cgcgccgccg cgaagcaagg cccgcaagat ttcaaaccca cctacgcctg
     5221 aaccgcggag catggccatg accgttctcg acgatctgcg taggctctct gccgccgagg
     5281 agttcttcag ctacctcggc gtggagagcg accccagcgt aatccgcgtc gccaggctcc
     5341 atatcctgcg ccgcatgggc gactacttgc ggggcgagca gttgagcggc ctcgacgacg
     5401 ccaccacgcg cgagcgttgc cgacaccacc tggtcgcggc ttacgaggac ttcgtgaaga
     5461 gcacaccgct cgagcagcgt gtcttcaagg tgttgaagga cgccgtggcg ccgaaaaagc
     5521 gggcgttcgt gccgctcgcc gccctctcca agcaggaaaa tgaggactga atgcacagcg
     5581 tcgtttgcat caagcaggtt ccggacagcg cgcaaatccg tgtccacccg gtgaccaaca
     5641 ccatcatgcg gcaaggggtg ccgagcatca tcaaccccta cgacctcttc gctctggaag
     5701 aggcgcttcg cctgcgcgat cggtacggtg gcgaggtcac ggtgctcacc atgggcccgc
     5761 ccatggccga ggactcgctg cgtaaggcgc tgacgttcgg tgcggatcgg gccgtactac
     5821 ttacggatcg gttcttcgcc ggctcggaca cgcttgctac gacgtacgcg ctcgcgaccg
     5881 caatcaagaa ggtcggggcc gaatggggca acccaacctt ggtcttcacc ggcaaacaga
     5941 ccatcgacgg agacaccgcg caggtcgggc ccgggatcgc caagcgcctc gggttgactc
     6001 agctcacgta cgtctcggag atcgaagaca tcgatctcca tcacgcgggc gtcatcgtgc
     6061 ggcgccgcgc cgaaggcgga gttcaacggc tttcgacgcg gctgccggcg ctggtcacgg
     6121 tgatcgaagg gatcaacgag attcggcggg ggaccatgac ggacgcgctg cgcgcggcgc
     6181 ggacgcagat cgtacgctgg agcgctgccg acgccggtgt ggaagaaatc gcgaagtgcg
     6241 gtcttcgcgg ctctccgacc atcgtcaagc aggtctacgc accaagcgct cgcgccgaga
     6301 aggccgagct cgttccgacc gagaatcagt cgtttcctca gatggcggac gcgctgttcg
     6361 gccgcatttt cgagaagaag ccggggctcg aggctgattt ggcaaagttc gtgagcgagc
     6421 gctgaagagc tcgcgtgggc tgaacgcaag ccgctcgtag gcttgaaagg ctcacgcgag
     6481 ccgcagcagg cggtactcca ccgctcgctc gaagatcctg gtgccgcccg cctgggctgc
     6541 gatggtcatg agctcaccgc catcggttac ggcgacccca gcgagatagc tgacaccgaa
     6601 gtcggccaac tccgagagcc acggcaggct cggtcccatc aagacgattt gcgcgccgcg
     6661 tgcttggcgg agcagcgaag gcagcgtctt gttgacgata gagctcgccg tcacgaacac
     6721 ccaatcggcg ccacgcagaa cctgctcggc gttgctttcg ggtacggttc ccggtagcgg
     6781 ccgtcgctcg aggcaaacgt actgatcggg gctccacacg cgctcgagcc ctgggtaccg
     6841 ccccaccacg acgacgcgag cgccttgaac ggcgctcttg aaatgctcga acaccgcgag
     6901 gtgcggcggc tggttcgatg aaagcggttt cgcgctttcg agcaaagcat tacccgggtg
     6961 gttgacgacg gcgttcacga cgagcgcgcc gaccgtcgcc gtggaagcgt ccaagctgcg
     7021 gatccacggc gcgagctcga tcgcgctccg cccaaccagc gtgccggacc agtcgagcgt
     7081 gcgcggcgcg ttcgtggggc tgaaacacag cccgtaagct tcgacttgcg cgacgctcca
     7141 gttcagcccg agaagaacgg aattcacttt ggatcgcgac tctgcacgcg cgatcaagag
     7201 gtcatagaga gattgcttca aggtaggttt gccaccggtt tagagggtga gcaccgatca
     7261 tgcaactagc gttctgtcga cgacccaaag acgcggcgtg agttcgagcg cgccctttcg
     7321 tcgtgaacct gtcggaaagc ggacgcacgt tcttttcgtg acatagccgg agatgaagtt
     7381 acgaagcgga tggcagcggc accgttcttg ctctcgtgct tgcagacctc atgaacatcg
     7441 aaggcgtcga gctcccggtc atttttcggt accacgcgtt ttgctgcttt cagcagcgcc
     7501 cgccggggca tcccaaaggt agctgcggcg cggcgggcgg gcccgagctt tgggagcact
     7561 tggggggcgc gattcagggc cgccaactcc aacgagttac gatgaccgcg actggctgtc
     7621 tcggtttctg caacgccggg ccgctcatgg tcgtgtaccc ggagggcgtt tggtatcagc
     7681 cgcgcacgaa ggaggacatc gacgagatcg tgcagtcgca cttcgtcgag ggtcgacccg
     7741 tggcgcgcct cgtggtggtg ctcgagcggt gacggctcat gcatccggcg cccctcgaac
     7801 ccgcgactga aacgcaagcc gagtcggcgc gcgcgcgcgt tcgccgctac cacgagggct
     7861 cgaagcatcg tctagatcgc tatgcgccag ggcccgaaac gctcgattgg gacggccaac
     7921 cttcgccgtt tcgacgtttt gcgggcgcgg cgcgcgtctc actcccatcg agcgctacga
     7981 gcgagggaaa gctcgccgag tcgctcgcgc ttccgttggg cgcggcgccg gacgag
//
LOCUS       DASTKS010001292         6415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1324712, whole genome shotgun sequence.
ACCESSION   DASTKS010001292 DASTKS010000000
VERSION     DASTKS010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324712"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6368)
                     /locus_tag="VFQ35_27735"
     CDS             complement(<1..6368)
                     /locus_tag="VFQ35_27735"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9934539.1"
                     /translation="MSLYEHLREDGVGCFTRADFPLSKLSQRELDAHRNRWWPRVVDA
                     HSLTAAQHAILVDVLREPHAGLYVPQLRATLPALDSRALERSLAWVIARHTALRTGFE
                     WQGLAEPVALVFENVACPITHEDFRSELESARRARLVEYLERDRARGFALREPPLLRL
                     LHVRWTDAEDVLVWTFHHAIADGWSVPLIVGEVLGAYRAFARGEVPELSPAPAFREYI
                     AWLLQQDEARGEAYFADALRGSPSVDSSWAERGAGPVVEVMRAVPRELSEALESLAKS
                     HAVTLGAVLQAAWAIVLGRYGDRRDVVFGSVDAGRPDEIARVAEMAGMFVTTLPLRVG
                     VDAELPVARLFADVQRASVGIREHGYVALARVVAANPGAASFESICVIENYPTLAGAL
                     GELTPRDVESFEWTRFPLTATASLHGSVTLSISASLADPSLGPELVGAWYGVLEQLSH
                     GATARVGDIRLAEAERRGAMARYWSSLLGDAPPTWLLGDRPRLGGAESSARAHVAVEL
                     DAASKRSLKAACASTGEPAELVFLGCFAALVARHAAQSELSMAVELEAGRRLPLVASV
                     ESASTLATIVRQIASFFAEARLNYDFSKAARWVERTGIGFVMQGVSDGPAQRRPRELE
                     LSLQVAAGEPSGFRLGYDTERFDEGSIRAFADELVALSLTLCDDIALPLARVPYFRAV
                     GAVSGSTDADESVRRWNVIQQFEARARATPDAIALISAEGELKYGDLARRVAAAREAL
                     LSRGVGPSELVCVHLPRSAALVTAILAVLEAGAAFVPIDIATPPERVRSTVEDCHARF
                     CLVDRESDALPAGVESIDVRRFALDGELGAARELAPDALAYVIYTSGSTGRPKGVMVE
                     HPALANYMEFYGRETQIGPGDRLLQFASPSFDVSIEEIFGALCHGATLVLRSDDVTPK
                     SFLGMARASSLTLLSVPTAYWAELTLALDAGSSLPESVRLVIVGGERAARATLAVWQR
                     KVSRGVRLLNMYGPTETTISATFADLTHEAADRVDPPIGKPVAGAIALVVDASGQAVS
                     VGVPGELLIGGRCLARGYLERAELTAQKFTPSPVGRVYHTGDLVRWRSDGQLEYLGRS
                     DQQVKIRGFRVELGEIEHAMRSVAGVEDVAVVLQGDDSEPPRLVAYVVGAAEPVALRE
                     GLKSLPSYMVPAAFVALSRLPLTRNGKLDRKALPAPARGEKASGRAATTALESRLLDI
                     WRRVLRLDRLGVDQNFFELGGHSLLATQIVARIQDELAIDAGVRDLFEAPTIESLAVR
                     LRAGAPNLTRREIAARNLDAAPLSFAELRLWFLDQFEPGSAAYNMPMALRLSGALDRV
                     GLEKALREVVRRHAVLRSVFVRGDDGQPLQRVLPDTTTEIVVDRLPAGPDPETWVRER
                     IQREVSEPFDLARGPLLRARLLAVGHLEHVLVCTVHHIVADGWSLSVFARELGACYAA
                     ALAGVESALPEPSIQYSDYALWQRQRFETLELESSLAYWKEELREAPLRLELPRKPRA
                     NGESLAGATLEMCLPKELSARVDALAVELGATPFIVLLSAFACLLARVSGERDLVVGT
                     PVANRRHVETEDLIGLFVNTLVVRADLRATPTFAELVAQLRERFLRAEAHQDLPFERL
                     VDALGVERDLERAPIFQVMFAMQNLPSVDFEVPGLSVALVPCTSGTAKFDVTLNVEAS
                     PEGYRTSWEYRSDLFEAAFVERMTRQFELLLSALVGAPLERWSRAPWFSDSERHTVLR
                     DWNHTERAYPEKRVHELIEEQAQRTPEAMAVRDEHGTLSYAELDRQSFALAQRLRAAG
                     VDRETRVGIALSRSRALVVGLLAVQRAGGAYVPLDPSLPRARLADVVEDAGVAVILTE
                     SGFREQLSSFAGVAIFDLEQPSRDDASMQSLPRHGIDDLAYVMFTSGSTGRPKGVEIT
                     HRALVNFLASMQDEPGIQPSDVVLAATSLSFDIAGLELYLPLVSGASVYVASREVATD
                     GRRLARAIDEFEVSFFQATPATYRLLLGAGWSGPTRSLKLLVGGEQLPLDVAQRLLDC
                     CDSLWNMYGPTETTIWSTCERVTRESLASDISIGRPIANTQAYVLDAELAPLPVGVVG
                     ELYLGGVGLARGYSGRPELTAERFLPNPFGPGRIYRTGDMARHLEDGRL"
BASE COUNT         1172 a         2220 c         2076 g          947 t
ORIGIN      
        1 agcctgccgt cctcgaggtg ccgggccatg tcacccgtgc ggtagattcg accgggccca
       61 aacgggttcg gaagaaagcg ctccgcggtg agctccggcc gacccgagta tccgcgcgcg
      121 agcccgaccc cacccaaata cagctcgccg acgacgccca cgggcagcgg cgcgagctcc
      181 gcgtcgagca cgtacgcttg ggtgtttgcg atcggacggc cgatgctgat gtcgctggcg
      241 aggctctcgc gagtgacgcg ttcacacgtc gaccaaatcg tggtctccgt cggaccgtac
      301 atgttccaaa ggctgtcgca gcagtcgagc agtcgctgcg caacgtcgag cggtagctgc
      361 tcgccgccta cgagcaactt caggctcctc gtggggccgc tccagcccgc gccgaggagc
      421 agtcggtacg tcgctggcgt cgcttgaaag aagctcactt cgaactcgtc gatggcacga
      481 gccaggcgac gcccgtccgt cgcgacctca cggctagcca catagacgga cgcgccgctc
      541 accagcggca gatagagctc gagcccagcg atgtcgaagc tcaggctcgt cgccgccaac
      601 acgacgtcgc tcggttgaat tccgggctcg tcctgcatgg aagcgaggaa gttcacgagc
      661 gcgcggtgcg ttatctcgac acctttcggt cgccccgtgg agcccgacgt gaacatcacg
      721 tacgcaagat cgtcgattcc gtgtcgggga agcgactgca tcgaggcatc gtcgcgagag
      781 ggctgctcga gatcgaaaat tgcgacgccc gcgaagctcg atagctgctc gcgaaacccc
      841 gattcggtga gaatgaccgc gacgccagcg tcttcgacca cgtccgccag gcgcgcgcgg
      901 ggcagactcg ggtccagcgg gacataagcg ccccctgccc tttgcaccgc caacagcccc
      961 acgaccagcg ctcgtgaacg gctcaaggcg atgccgacgc gcgtctcgcg gtcgacgccc
     1021 gcggcgcgaa gccgctgcgc gagcgcgaag ctttgacggt cgagctcggc gtagctgagc
     1081 gtgccgtgtt cgtcgcgcac cgccatcgcc tccggcgtgc gctgcgcttg ctcttcgatg
     1141 agctcgtgga cgcgcttctc cgggtacgct cgctcggtgt ggttccagtc gcgcaggacg
     1201 gtgtgccgct ccgagtccga gaaccagggc gcacgcgacc agcgctccag aggagcgccg
     1261 accaatgcgc tgaggagcag ctcgaactgt cgcgtcatgc gctcgacgaa ggccgcctcg
     1321 aacagatccg atcgatactc ccaagaagtt cggtagcctt cggggctggc ctccacgttc
     1381 aacgtcacgt cgaacttggc ggtaccggag gtacacggca cgagcgcaac ggagagcccc
     1441 gggacttcga agtccacgct cggcaggttt tgcatcgcga acatcacctg aaaaatcggc
     1501 gcgcgctcca aatctcgctc cacgccgagc gcgtcgacga gcctctcgaa gggtaaatct
     1561 tgatgcgcct cggcgcgcaa gaagcgctcg cgaagttgcg ccacgagctc ggcgaaggtc
     1621 ggtgtcgcac gcaaatccgc tcggacgacc agcgtgttca cgaagagccc gatgaggtct
     1681 tcggtctcga cgtgacggcg attggccact ggcgtaccga cgaccaggtc tcgttcaccg
     1741 ctcacccgcg cgagcaaaca agcaaacgcc gacaaaagaa cgatgaacgg cgtcgccccg
     1801 agctcgaccg ctagggcgtc gacgcgcgcc gatagctctt tggggaggca catctcgagg
     1861 gtggcccccg cgagactctc gccgtttgcg cgcggcttgc ggggcagctc gagccggagc
     1921 ggcgcttcgc ggagctcttc tttccagtag gcgagcgagc tttcgagctc gagcgtctcg
     1981 aacctctgac gctgccaaag ggcatagtct gagtactgaa tcgacggctc cggcagcgcg
     2041 ctctcgacgc ccgctagcgc cgcggcgtag caggcgccga gctcccgcgc gaagacgctg
     2101 agcgaccagc cgtcggcgac gatgtgatgc acggtgcaga cgagcacgtg ttccagatgt
     2161 cccaccgcga gcaggcgcgc tcgcaagagc ggtccgcgcg cgaggtcgaa tggctcactc
     2221 acctcgcgtt gaattcgctc gcgtacccac gtttcggggt ctggcccggc gggcagacgg
     2281 tcgacgacga tttcggtcgt cgtgtccggc aggacgcgct gcagcggttg tccgtcgtcg
     2341 ccgcgcacaa agacgctacg cagcacggcg tgacgccgaa ccacctcgcg cagcgccttt
     2401 tcgaggccca cgcgatcgag cgcgccggac aagcgcagag ccatgggcat gttgtacgcg
     2461 gcgctgcctg gctcgaattg atccaaaaac caaagacgta gctctgcgaa cgaaagcggc
     2521 gccgcgtcga ggttccgggc cgcgatctcg cggcgagtca agttcggagc ccccgcccgt
     2581 agacgcaccg cgaggctctc gatggtcggc gcctcgaaca agtcgcgcac ccccgcgtcg
     2641 atcgcgagct cgtcctgaat tcgcgcaacg atttgagttg cgagcaacga atgcccaccg
     2701 agctcgaaga agttctggtc gacgcccagc cgatcgaggc gcagaacgcg ccgccagatg
     2761 tcgagcaggc gagactcgag cgctgtcgtc gcagcgcggc cactcgcttt ctcgccgcgc
     2821 gcaggagctg gcaatgcttt tcgatcgagc ttgccgtttc gggtgagcgg aagccgcgag
     2881 agcgccacga aagccgccgg caccatgtac gacggcaacg acttcagccc ttctcgcaaa
     2941 gcgacaggct ctgcagcccc gaccacgtaa gcgaccaggc gcggcggctc ggagtcgtcg
     3001 ccttgcaata cgaccgccac gtcttcgacg cccgcgaccg acctcatcgc atgctcgatc
     3061 tcgccgagct cgacgcggaa gccgcggatc ttcacttgct gatcgctacg gccgaggtat
     3121 tcgagctgcc catcggagcg ccagcgcacc aaatccccgg tgtggtagac gcgtccgacg
     3181 gggctcggcg tgaacttctg cgcggtgagc tccgcgcgct cgagatagcc tcgggcgagg
     3241 cagcggccgc caatcaagag ctcgccgggt acgcccaccg aaaccgcctg accgctcgcg
     3301 tcgacaacca gcgcgatcgc gccggcaact ggtttaccga tgggcgggtc gactcggtcc
     3361 gcagcttcgt gcgtgagatc tgcgaacgtc gccgagatcg tcgtctcggt aggcccgtac
     3421 atgttgagga gccgcacccc cctcgacact ttgcgctgcc aaacggccag agtggcgcga
     3481 gccgctcgct ccccaccgac gatgacgagc cgaacacttt cgggcagtga agaccctgcg
     3541 tcgagcgcca gcgtgagctc cgcccagtag gcggtgggta cgctgagcag agtgagggac
     3601 gacgcccggg ccatcccgag aaaactcttg ggagtgacgt cgtcggagcg cagcacgagc
     3661 gtcgcaccgt gacagagcgc gccgaaaatc tcctcgatgc tgacgtcgaa gctcggcgaa
     3721 gcgaactgca agagccgatc gcccggacca atctgcgtct cgcgcccata aaattccatg
     3781 tagttggcga gcgccgggtg ctcgaccatc actcccttgg ggcggccggt agaaccggag
     3841 gtgtagatca cgtacgcgag cgcatcggga gcgagctctc gagccgcccc gagctcacca
     3901 tctagcgcga agcgacggac gtcgatcgat tcgacgccgg ccggcaacgc atcgctctcg
     3961 cgatcgacga ggcaaaaccg agcgtgacaa tcttcgaccg tggagcgcac gcgctcggga
     4021 ggcgtcgcga tgtcgatcgg gacgaacgcc gcgcccgcct ccaaaacagc cagaatggct
     4081 gttacgagcg cggccgagcg cggcaggtgt acgcagacga gctccgaagg ccctacgccc
     4141 cgcgaaagga gcgcctcgcg tgccgcggcg actcgccgcg caaggtcccc gtacttgagc
     4201 tcgccctcgg ccgaaatcag cgcgatcgcg tccggcgtcg cccgtgcgcg cgcctcgaac
     4261 tgctgaatta cgttccagcg ccgaacggac tcgtctgcat ccgtcgagcc gctcacggct
     4321 cccaccgcac gaaagtacgg aacccgcgcg agcggcaacg cgatgtcgtc gcacaaggtt
     4381 agcgagagcg cgacgagctc gtccgcgaac gctcgaatcg aaccttcatc gaagcgctcg
     4441 gtgtcataac cgagacggaa cccgctcggc tcgcccgcgg ctacctgcaa actgagttcg
     4501 agctcccggg gccgcctttg ggcaggacca tctgacacgc cctgcatgac gaagccgatg
     4561 ccggtgcgct cgacccaacg agccgccttg ctgaaatcgt agttcagccg agcttcggcg
     4621 aaaaagctcg cgatctgccg aacaatcgtc gcgagcgtgg aggcgctttc gacggaagca
     4681 accaggggca accgacggcc ggcctcgagc tcgaccgcca tgctgagctc cgattgagct
     4741 gcatggcgcg ccacgagcgc agcgaagcag cccaagaaga cgagctctgc gggctcgcca
     4801 gtgctcgcgc acgcggcttt taggctcctc ttcgacgccg catcgagctc gaccgcgacg
     4861 tgagcgcgcg cactcgactc ggctcctccg agtcgcggac gatcgccgag cagccacgtc
     4921 gggggcgcgt ctccgagcag cgagctccag tagcgcgcca tcgccccacg ccgctcggcc
     4981 tccgcaagcc gaatgtcgcc gacccgcgcc gtcgcgccgt gcgacagctg ttcgaggacg
     5041 ccgtaccaag caccgacgag ctccgggccg aggctcggat cggcgaggct cgcgctgatg
     5101 ctcagcgtga ccgagccgtg caaagacgcc gtcgccgtca acgggaagcg agtccactcg
     5161 aagctctcga cgtcgcgcgg agtcagctcc cccagtgcgc cggcgagcgt cggataattc
     5221 tcgattacgc agatcgactc gaacgacgcc gcgcccgggt tcgcagcgac gaccctcgcc
     5281 aaggcgacgt agccatgctc gcggataccg acgctcgcgc gttgcacgtc cgcaaacaag
     5341 cgcgcgaccg ggagctccgc gtcgacgcca acccgcagcg gcagcgtcgt tacgaacatg
     5401 ccggccatct cggccacgcg tgcaatttcg tcgggacgtc ccgcgtcgac gctgccgaag
     5461 acgacgtcgc gacggtctcc gtagcggccc agaacgatcg cccacgccgc ttgaagcacc
     5521 gcgcccagcg ttaccgcatg gctcttggca aggctttcca gcgcctcgga aagctcgcga
     5581 ggaaccgcgc gcatcacctc gacgaccggc ccggcgccgc gctcggccca gctcgaatcg
     5641 actgagggac tgccgcgcag tgcgtccgcg aagtacgcct cgccgcgcgc ctcgtcctgt
     5701 tgcagaagcc aggcgatgta ctcgcggaag gcgggcgccg ggctcagctc gggcacctcc
     5761 ccgcgcgcga atgcccggta tgcgccgagc acctcaccga cgatcaaagg tacgctccaa
     5821 ccatccgcga tcgcatggtg gaatgtccaa acgagaacgt cctccgcgtc ggtccagcgc
     5881 acgtgcagca agcgcaggag cggcggctcc cgtaaggcaa agccgcgcgc gcgatcgcgc
     5941 tcgaggtact ctaccaagcg tgctcggcgg gcgctttcga gctcgctgcg aaagtcctcg
     6001 tgcgtgatcg ggcacgcgac gttctcgaag acgagcgcca ccggctcggc aaggccctgc
     6061 cactcgaagc cggtgcgcaa cgcggtgtgg cgagcgataa cccacgcgag gctccgctcg
     6121 agcgcgcgcg agtcgagcgc tggtaacgtg gcgcgaagct gcggcacgta gagtcccgcg
     6181 tgcggctccc gcaacacgtc gaccaaaatg gcgtgctggg ccgcggtgag cgagtgggca
     6241 tcgacgactc gcggccacca gcggttgcgg tgcgcgtcga gctcccgctg gctcagcttg
     6301 ctcagaggga agtcggcccg cgtgaaacag ccgacaccat cctcgcgcaa atgttcgtag
     6361 aggctcacga gagcggcgtc aaaagccgcg agcagccgat tcatgctctc gggca
//
LOCUS       DASTKS010001293         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1548477, whole genome shotgun sequence.
ACCESSION   DASTKS010001293 DASTKS010000000
VERSION     DASTKS010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548477"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /gene="ptsP"
                     /locus_tag="VFQ35_27740"
     CDS             complement(<1..1263)
                     /gene="ptsP"
                     /locus_tag="VFQ35_27740"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9934540.1"
                     /translation="MSERPGVPASSPGSQCVVLDGIAGSPGYAIGKTLVVDTRRHGFP
                     RRHIPKHLADDEVERFTRAVAAAARELREVEERARSRATRLESSILAAYLLMVEDETL
                     HEEVERRIRIDHLCAEWALSATIEEMATQLRQGGDPYLAERSHDFEFVGDRIIGALAG
                     RASILALPGDRSPCILVAHDLSPAETAGLTRDRVLGIVTEVGTRTSHTSILARALEIP
                     AVVGVNGLLSRVGDGDLLAVDGVHGRVIVNPFAELMAEHQDRSRRFEAAVRARQKLRD
                     RPIVTRCGATVDLRANIELPNEAELALAQGARGIGLYRTEFLYVDRSEPPSEDEHYET
                     YRRVVETVAPLPVTLRTFDIGGDKFVSSLQLPTDMNPALGLRAVRLGLARPDLMLTQL
                     RAMIRASAHGPMRIMVPMIASLGELRAVR"
     gene            complement(1267..1542)
                     /locus_tag="VFQ35_27745"
     CDS             complement(1267..1542)
                     /locus_tag="VFQ35_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9934541.1"
                     /translation="MTPARGTFEVRNKLGLHARAATKLVQLASRFPCDIVISRAGQSA
                     NAKSVMGVLLLCGSKGTKLDIEARGTDAEQAVTEIGKLISDRFGEDE"
     gene            complement(1539..2519)
                     /gene="rapZ"
                     /locus_tag="VFQ35_27750"
     CDS             complement(1539..2519)
                     /gene="rapZ"
                     /locus_tag="VFQ35_27750"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9934542.1"
                     /translation="MRPSARPPGPIAVTVVTGVSGAGKSTAVHALEDLGFFCVDNLPS
                     PVLASTMDALSRAGLTRVAFGIDVRVRGFLDDAASAINELSRSEDRNVEVLFLDASDE
                     ALLRRFSSTRRPHPLSTTVEPGGEVSAIAVLDGIRLERELLNPLRARATIVLDTTSLS
                     VNELRRRVREHFAPGAGRLPRVYVRFVSFGFKFGTPVDADVVLDVRFLQNPHFVPELR
                     PLSGLDKRVSEYVLNSEDGPGFVSRATELLEYCLPRFEREGRSYVTIAIGCTGGRHRS
                     VALASYFADTIGKNLNLPIEVMHRDIDRVSMKGLGADPDHSVEPTPGGKG"
     gene            complement(2516..3586)
                     /gene="hprK"
                     /locus_tag="VFQ35_27755"
     CDS             complement(2516..3586)
                     /gene="hprK"
                     /locus_tag="VFQ35_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET9934543.1"
                     /translation="MRPSPEPPELARGVSVRDLLDDPALGLNVRLVAGGNGLGRIIDH
                     PRIQKSGLSLVGHVRGVVPSRIQVLGETELSYAETLSPESQRLAAECLFSLNLACVLV
                     TRGVDPPRPFSDAAERTGTPLVVCAERSSAVIAAVHALLDERLAPRTRIHGVLVDVFE
                     VGVLLLGKSGIGKSECALELVMRGHRLVADDVIECDYRPPAMVFGQPAALLRHHIEVR
                     GLGVLNIKDLFGVTAIRERKRIDLVVELSLWQPDADYDRIGLEDRHREILGIPVREVV
                     LPVRPGRNMSSIIEIAARNELLRQAGHHSAREFVQRLETGLLRSDDIPQTSRPPSSQF
                     RSPLPPNESSAPPPVLPVERRR"
     gene            complement(3583..>3915)
                     /locus_tag="VFQ35_27760"
     CDS             complement(3583..>3915)
                     /locus_tag="VFQ35_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9934544.1"
                     /translation="LLERERLQSTGIGDGVAIPHTAVEEAATQAAAVVLCPKGIDFEA
                     IDGEKVRIVFGVVGPKRATGEHLRTLARISRLLRDVATREKLVSSPNASAALELIQTN
                     DARAEGKT"
BASE COUNT          680 a         1322 c         1310 g          603 t
ORIGIN      
        1 tctcaccgcg cggagctcac cgagcgaggc gatcatgggg accatgatgc gcatcgggcc
       61 gtgcgccgag gcgcggatca tcgcgcggag ctgggtgagc atcagatcgg gccgcgcgag
      121 gccgaggcgc accgcgcgca agccgagggc cgggttcatg tcggtcggta gctgcaacga
      181 cgagacgaac ttgtcgccgc cgatgtcgaa cgtgcgcagc gtgaccggta gcggagccac
      241 cgtctccacg acgcgccggt acgtctcgta gtgctcgtct tcgctcggcg gctcggagcg
      301 atcgacatag agaaactcgg tgcgataaag gccgatcccg cgggctccct gggctagcgc
      361 gagctcggct tcgttcggga gctcgatgtt cgcgcgcaaa tcgacggtcg ctccgcaacg
      421 cgtcacgatc gggcgatcgc gcagcttttg gcgagcgcgc acggccgctt cgaagcgccg
      481 tgagcgatcc tggtgctccg ccatcagctc ggcgaaggga ttgacgatga cgcggccgtg
      541 cacgccgtcg accgcgagga ggtcgccgtc accgacgcgg ctgaggagcc cattcacgcc
      601 gacgacggcc gggatctcga gggcgcgcgc caaaatcgaa gtatggctcg tgcgcgtgcc
      661 gacctccgtg acgatgccga gcacccgatc gcgcgtcagc cccgcggttt cggcgggaga
      721 caaatcgtgc gcgacgagga tgcacggcga acgatcgccg gggagcgcca agatcgacgc
      781 gcgccccgcc agagctccga tgatgcggtc gccgacgaac tcgaagtcgt gactccgctc
      841 ggcgagatac ggatccccgc cttggcggag ctgggtcgcc atttcttcga tggtcgcaga
      901 gagcgcccac tccgcgcaca gatgatcgat gcggatccgt cgctccacct cttcgtgcag
      961 cgtctcgtct tcgaccatca gcaaatacgc cgcgaggatc gagctctcga gccgtgtcgc
     1021 acgggagcgc gcccgctctt cgacctcacg cagctcgcgg gcggcggcgg cgacggcgcg
     1081 cgtgaagcgc tcgacctcgt cgtcggcaag gtgcttcgga atgtgacgtc gcgggaagcc
     1141 atgtcggcgc gtgtcgacga ccagggtctt gccgatggcg tagccaggcg agcccgcgat
     1201 gccgtccagc accacgcact gtgaccccgg cgacgaagcc ggcacgcccg gacgctccga
     1261 catgctctac tcgtcttctc cgaaacgatc cgaaatcagc tttccgattt cggtcacggc
     1321 ttgctccgca tcggtgccgc gcgcctcgat gtcgagcttg gttcctttgg agccgcacaa
     1381 gagcagcacc cccatcacgc ttttggcgtt ggcagactgc cccgcacggc taatcacgat
     1441 gtcgcatgga aacctcgaag cgagttgcac gagcttcgtc gccgcacgcg cgtgcagtcc
     1501 gagcttgttt ctcacttcga acgtccccct cgctggcgtc atcctttacc tcctggcgtg
     1561 ggttccacac tatgatcggg gtcggcgccg agacctttca tgctgactcg gtcgatgtcg
     1621 cggtgcatga cctcgatggg caagttcaag ttcttgccga tcgtgtccgc gaagtagctg
     1681 gcgagagcca ccgagcgatg acggccgccg gtgcagccga tggcaatcgt aacgtaactc
     1741 cggccctctc gctcgaagcg cgggaggcag tactcgagca actcggtggc acgagagacg
     1801 aatcccggcc cgtcttcgga gttcagcacg tactcgctga cgcgcttgtc gagacccgac
     1861 agggggcgca actccggcac gaagtgagga ttctgcaaga agcgaacgtc cagcacgacg
     1921 tcggcgtcca ccggagtacc aaacttgaaa ccgaaagaga cgaaccgaac gtagacacgc
     1981 ggcagccggc ccgccccagg ggcgaagtgc tcacgtaccc ggcggcggag ctcgttcacc
     2041 gacaggctcg tcgtgtcgag cacgatggtc gcgcgcgctc gcagcgggtt gagcagctcg
     2101 cgctcgagcc gaatgccgtc cagcaccgca atcgccgaaa cttcgccgcc gggctccacc
     2161 gtggtgctca gcggatgcgg ccttcgggtg ctgctgaaac gcctcaacaa cgcttcgtcc
     2221 gaagcgtcca agaacaggac ctcgacgttg cggtcctccg agcgagacag ctcgttgatc
     2281 gccgacgcgg cgtcatccaa gaagccacgc acgcggacgt cgatgccaaa ggcgactcgc
     2341 gtgaggccgg cgcgggagag cgcgtccatc gtgctcgcga gcacgggcga cggcagattg
     2401 tcgacgcaaa agaagcccag atcttcgagc gcgtgcacgg cggtcgactt gcctgcaccg
     2461 ctcacgcccg tgacgacggt tacggcgatg gggcccggcg ggcgcgcaga gggcctcatc
     2521 ggcgtcgctc caccggcaag acggggggcg gcgcggagct ctcgttgggc ggcagcggcg
     2581 accggaactg cgagctcggc ggacgcgacg tctgggggat gtcgtcggag cgcaaaagcc
     2641 ccgtttcgag ccgctgaacg aactcgcgcg cggagtggtg gcccgcttgg cgcaagagct
     2701 cgttgcgcgc cgcgatctcg atgatgctgc tcatgttgcg tccgggccgc accggcaaca
     2761 cgacttccct caccggaatg ccgaggatct cgcggtggcg gtcctcgaga ccgatgcgat
     2821 cgtagtcggc gtcgggctgc cacagcgaca gctccaccac gagatcgatg cgcttccgct
     2881 cgcgaatcgc ggtcaccccg aataaatcct tgatgtttag cacgccaagt ccgcgcacct
     2941 cgatgtggtg gcgcagaagc gccgccggct gcccgaacac catcgcgggc gggcggtagt
     3001 cgcactcgat cacgtcgtcg gcaaccaacc ggtggccgcg catgacgagc tcgagggcgc
     3061 actcgctctt gccgatgccg ctcttaccga ggagcagcac gccgacctcg aacacgtcga
     3121 cgagcacccc gtgaatgcgc gtccgaggcg ccagccgctc atcgagcagc gcgtgcacgg
     3181 cggcgatcac ggcggaagag cgctcggcac agacgacgag gggcgtaccc gtgcgctccg
     3241 cggcgtccga aaatgggcgc ggcgggtcga ccccgcgcgt caccaacacg caggcgagat
     3301 tcagcgagaa caagcactcc gccgcgagcc gctgggattc tgggctcagc gtttcggcgt
     3361 agctgagctc ggtctcgccg agcacctgga tgcgcgaggg aacgacgccg cgcacatggc
     3421 cgacgagaga aagcccggac ttctgaatcc gagggtgatc gatgatacgc ccgagcccat
     3481 tgccccccgc gacgagcctg acgttcaggc cgagagcggg gtcatccagc agatcgcgca
     3541 cggagacacc gcgcgcaagc tccgggggct cgggcgacgg cctcacgtct tgccctcggc
     3601 gcgcgcgtcg ttcgtttgaa tcagctcgag cgcggcgctc gcgttcggtg acgacacgag
     3661 cttctcccgc gtcgcgacgt cgcgcagcag ccgggaaatg cgcgccaacg tccgcaggtg
     3721 ctcgcccgtc gcgcgcttgg ggccgaccac gccgaagacg atgcgcacct tctcaccgtc
     3781 gatggcttcg aagtcgatcc ctttgggaca cagcaccacg gccgccgctt gcgtggcggc
     3841 ctcctctacg gccgtgtgag gaatcgcgac gccatcaccg atgccggtgc tttgcaggcg
     3901 ctcgcgctcg agcaa
//
LOCUS       DASTKS010001294         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_207845, whole genome shotgun sequence.
ACCESSION   DASTKS010001294 DASTKS010000000
VERSION     DASTKS010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207845"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VFQ35_27765"
     CDS             complement(<1..1342)
                     /locus_tag="VFQ35_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9934545.1"
                     /translation="MNDKPATYLRVGAEAPSFAERVWVALGLPGRIGVVVFGVVLVLF
                     LGYLAARRLAGGGLWRRILYLLTAVGIVGLLGLGTWAARLPEPRGTYFVLWHQFVRGG
                     AALSGTLFLVGFMGLAVPKLLARFEGRGYISFVASRHVLSQKSGFLTVISVLSIAGVL
                     LSSLALCAVVAIMGGFGADLKRKIVGNNAHIRIEGARDSGIPNWQSSLEKLRLIPGVH
                     AATPVAGGEAMASSATNTAGVIVRGVDVVTIGTVINLISNIEVGQFKYLEHPELLDKM
                     GPEEPIGLGPGGELYLKGPDIKPYLKKVDPDVEDAVKERSYPGIIIGRELAKSMHVYV
                     GDEVTLVSPLGELGPMGLLPKARRFRVAAIFYSGMYEYDATQAYTTLEAAQDFFDLKN
                     VVTALDIRVDEPERVDEVRPLVVKALDEPKLRVRDWKEMNKNLFSALKLEKIATF"
     gene            complement(1339..>2247)
                     /locus_tag="VFQ35_27770"
     CDS             complement(1339..>2247)
                     /locus_tag="VFQ35_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9934546.1"
                     /translation="VYEIGRCYRNEGISTRHNPEFTMLEYYQAYATYETLMDQTEVML
                     REVDMALGARLEALGLKANYEAFVASRPFTLAEPFARVPMHTAVVNSLERAGLGREVL
                     SKLSSLAATPREKGAPPTAEDTVVAGYKGTSERAGRIDWPNLRRALAHCKSDGERLFV
                     AYEYFAEPFLAEDYRSADGKLSRPVFIMDYPFEVSPLARRKTADPSLTDRFELFVHSR
                     ELCNAFSELNDPDDQAARFRAQVEAKASGAEETMDYDVDYVRALEHGMPPAAGFGLGV
                     DRLVMLLTGQASIRDVIAFPLLRPEE"
BASE COUNT          420 a          739 c          724 g          364 t
ORIGIN      
        1 tgaacgtcgc gatcttttcg agcttcagcg cgctgaaaag gttcttgttc atctccttcc
       61 agtcgcgaac gcgaagcttc ggctcgtcga gcgccttcac gactagcggc cgcacctcat
      121 cgacccgctc cggctcgtcg acgcgaatgt cgagggcggt gacgacgttc ttcaagtcga
      181 agaagtcctg cgcggcttcg agcgtcgtgt acgcctgcgt ggcgtcgtac tcgtacatgc
      241 cgctataaaa gatggccgcg acacggaagc gccgggcctt cggcaaaaga cccatcggac
      301 cgagctcgcc caacggcgag accagcgtga cctcgtcgcc cacgtaaacg tgcatcgatt
      361 tagcgagctc tcgcccgatg atgatccccg gataagagcg ctctttgacc gcatcttcga
      421 cgtcggggtc gaccttcttc aagtagggct tgatgtcggg ccccttcaag tagagctcgc
      481 cacccggacc gaggccgatg ggctcttcgg gacccatctt gtcgagcagc tccgggtgct
      541 cgaggtactt gaactggccg acctcgatgt tgctgatgag gttgatgacg gtgccgatcg
      601 tgacgacgtc cacgccacgc acgatgacgc ccgcggtgtt cgtggcgctc gacgccatgg
      661 cttcgccgcc cgccaccggc gtcgcggcgt gcacgcccgg aatgagccgg agcttctcca
      721 ggctgctctg ccaattcggg ataccgctgt cgcgcgcccc ctcgatgcga atgtgcgcgt
      781 tgttgccgac gattttgcgc ttcaggtcgg cgccgaaacc gcccatgatc gcgaccaccg
      841 cgcagagcgc gagcgaactc aagaggacgc cggcgatcga gagcacgctg atgaccgtca
      901 agaacccgct cttttgagag agcacgtggc gcgacgcgac gaaggagatg tatccccgtc
      961 cctcgaagcg cgcgagcagc ttcgggacgg cgagacccat gaagccgacc aagaacagcg
     1021 tccccgaaag cgccgcgcca ccacgcacga actggtgcca cagcacgaag taggtgccgc
     1081 gcggctccgg caagcgggcg gcccacgtac cgagcccgag aaggcccacg ataccgacgg
     1141 cggtgagcag gtacaggatc cggcgccaga gcccgccgcc agcgagccga cgcgccgcca
     1201 agtacccgag aaaaagcacg agcacgacgc caaagaccac caccccaatg cgtccgggca
     1261 gccccaaggc gacccacacg cgctcggcaa acgatggtgc ctcggcgccg acgcgcaggt
     1321 acgtggcggg tttgtcgttc attcttcggg ccgcaagagc gggaacgcga tcacgtcgcg
     1381 aatcgatgct tggccagtga gcaacatcac gaggcgatcg acgcccaagc cgaagccggc
     1441 cgccgggggc atgccgtgct cgagcgcgcg cacgtagtct acgtcgtagt ccatcgtctc
     1501 ctcggcgccc gacgccttgg cctcgacctg agcgcggaag cgagcggcct ggtcgtcggg
     1561 atcgttcagc tcactgaagg cgttgcagag ctcacgtgag tgcacgaaga gctcgaagcg
     1621 atcggtgagc gaggggtcgg cggtcttgcg gcgcgcgagc ggagacacct cgaagggata
     1681 gtccatgatg aagacggggc ggctcagctt gccgtcggcg cttcggtagt cttcggccaa
     1741 aaacggctcg gcgaagtact cgtaggcaac gaataagcgc tcaccgtccg acttgcagtg
     1801 cgcgagcgca cgccgaaggt tgggccaatc gatgcggccc gctcgttcgg aagtgccctt
     1861 gtaaccagcg acgaccgtgt cttccgcggt tggcggagcg cccttttcgc gcggggtcgc
     1921 agcgagcgac gaaagcttcg aaaggacctc ccgaccgagg ccggcgcgct cgagcgagtt
     1981 gaccacggcc gtgtgcatcg gaactcgcgc aaacggctcg gccagggtga acgggcgcga
     2041 cgcgacgaac gcctcgtagt tcgccttcaa tccgagcgcc tcgagccgag cgcccagcgc
     2101 catgtcgacc tcgcggagca tcacctccgt ttggtccatc agcgtctcgt acgtcgcgta
     2161 cgcctgatag tactcgagca tcgtgaactc cgggttgtgc cgggtgctga tgccctcgtt
     2221 gcggtagcag cggccgatct cgtaaac
//
LOCUS       DASTKS010001295         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_653921, whole genome shotgun sequence.
ACCESSION   DASTKS010001295 DASTKS010000000
VERSION     DASTKS010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653921"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFQ35_27775"
     CDS             <1..1057
                     /locus_tag="VFQ35_27775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9934547.1"
                     /translation="TAATAVLLAQEGHLDLQRPIATYLPELDANAELGRVTLHQLLTH
                     TAGLVDDPRRPLCEGARALSDATVLAHVGARPGTVYLYSNFGYALAGLVIERTTSRAF
                     ADVVRERVLLPMGMSTATFDFARVTLRGHPEGSGDKSRCLLAGPAGGLIASTRDLARW
                     ARAMSEPATHPLGQRLVETLTTPYVETGARPDEAYGYGVWITKHGGVSVYHHGGGVQD
                     FSAYIAWVPARRLGTAAAMNFGHAAMIDRVGGTLGVVVLRELSVLLDLPNDWRATLES
                     PRRPLSAYVGTYVDRKSWLGRLCVRLVGSDQLAFDYPDGPPALLPASFAFRFAPGEQR
                     ARFVVTAVGVAERVGDD"
     gene            complement(1075..2802)
                     /locus_tag="VFQ35_27780"
     CDS             complement(1075..2802)
                     /locus_tag="VFQ35_27780"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934548.1"
                     /translation="MTSAVRVSLFRAALLVLGFTVFACYRPTRRECAVLCGDGNQCPD
                     GMDCSNGRCTSHGRQCSRLETDAAPPPVECVPGTTRCASRAAVERCDMDGVTWSQVQA
                     CGDETPVCAAGQCLRCTPGSARCIDETSRAVCDETGNFGEPSRCPAGANSCVNAECTP
                     KLQVAAGGAHACAKLASGRVFCWGENSAGQLGVGDRLRRRGDPSDLPLEAVELGSPAL
                     SVVTGGAHTCALLEGGVVKCWGENAAGQLGQGDVERRGDEPGEMARLRPVDLGAARTA
                     VSLTAGDRHTCALLDDGTVKCWGANESGQLGVSGARHRGGAPFELGERLTPLALDWDT
                     VVVAIDAGAAHTCAALADGSVVCWGDNSAGQLGIGAANGSDGALQSGRLVPRVILGAE
                     RRAVGVAAGADHSCALIEGGSVVCWGNNDAGQLGVGDAVSRGRQPEGIEAELSEVELG
                     SGAFALAVSAGARHTCVLLAQGAVKCWGSNAFGALGLGRAELNRGVMPSDLGDALLTV
                     DLGSRQSGARAVSGLSAGLDFTCAVLEDEAIKCWGLNAHGQLGLGDSRNRGLSPGELG
                     ELLPEVVLE"
     gene            complement(2799..>3329)
                     /locus_tag="VFQ35_27785"
     CDS             complement(2799..>3329)
                     /locus_tag="VFQ35_27785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934549.1"
                     /translation="IRLCRCSVPADAFKPLTTDPLDRAQFYAVLKFGDSCPKHAVEVT
                     KVIVNEDQDTQNAPTNTPELLYPNEIVDRALGNFTRLVFCYFRNAPSADDTMSEFPDL
                     GVSYAVFHDFEGPQPGWVIRKRWQLSDDSNTAFPGNAYYADPPRDDIIAGFMDIIENP
                     VRGTTHDTYFDMARVR"
BASE COUNT          566 a         1153 c         1062 g          548 t
ORIGIN      
        1 cacggcggcg accgctgttc tgctcgctca agaggggcac ctcgaccttc aacgaccgat
       61 cgccacgtat cttcccgagc tcgacgcgaa cgctgaactc ggccgcgtca cgcttcacca
      121 actcctgacg cacaccgcag ggctcgtgga cgatccgcga cgcccgctgt gcgagggggc
      181 ccgagccctg agcgacgcca ccgtgttggc acacgttgga gcgcgaccgg gcacggtcta
      241 cctttactcg aacttcggat acgcgctcgc ggggctcgtg atcgagcgaa cgacgtcgcg
      301 agccttcgcc gacgtcgttc gtgaacgagt cctcttgccg atggggatgt ctacggcgac
      361 gttcgatttt gcgcgggtca cgcttcgagg acatccggaa ggctctggcg acaaaagccg
      421 gtgtcttctg gccggtccag ccggcggcct gattgccagt acgcgcgacc tcgctcgttg
      481 ggcccgcgcc atgagcgaac ccgccaccca tcccctaggt cagcggctcg tcgagacgct
      541 cacgacgccg tacgtcgaga cgggagcgcg gcccgacgaa gcctacggct atggcgtctg
      601 gatcacgaaa cacggcggcg tgtccgttta ccaccacggc ggcggagtcc aagacttcag
      661 cgcctacatc gcctgggtac cagcgaggcg gctcgggacc gccgcggcga tgaacttcgg
      721 acacgcggcg atgatagata gagtcggtgg cacgctcggc gtcgtcgttc ttcgcgagct
      781 cagtgtgctc ttggacctgc cgaacgattg gagagccacc ctcgagagcc cgcgacgtcc
      841 cttgagtgcg tacgttggca cttacgtgga ccgcaagagt tggctcggtc gtctgtgcgt
      901 acgtctcgta ggctccgacc agctcgcgtt cgactacccc gacggtccgc cagccctcct
      961 accggcatca ttcgcattcc gctttgcccc cggcgagcaa cgcgcgaggt tcgtcgtcac
     1021 ggcggtgggc gtcgccgagc gcgttggcga cgattgaccc ctgctaactt gtcttcactc
     1081 gagaacgacc tcaggcagga gctcgccgag ctcgccgggg ctcagcccgc gattccgaga
     1141 atcacccaac ccaagctgcc cgtgtgcgtt gaggccccaa catttgatgg cttcgtcctc
     1201 gagcacggcg cacgtgaaat cgaggccggc gctgaggccc gaaaccgcgc gggcgccgct
     1261 ctgtcgagac ccgaggtcga ccgtgagcaa ggcgtcaccg aggtcgctcg gcatgacgcc
     1321 acggtttagt tccgcgcgac ccaagccgag cgctccgaag gcgttcgacc cccagcactt
     1381 gaccgcccct tgcgccaaga gcacgcaggt gtgcctcgcc cccgcggaga ccgcgagagc
     1441 gaacgctccg ctgccgagct ctacttcgct gagctccgcc tcgatgcctt cgggctgccg
     1501 accacggctc accgcgtcgc caacgccgag ttggcccgcg tcgttgtttc cccaacacac
     1561 aaccgagccg ccttcgatta gcgcacagga atgatccgcg cccgctgcca ccccaaccgc
     1621 gcgccgctcg gctccgagaa tcacgcgggg gacgagcctg ccgctttgga gggcaccatc
     1681 cgagccgttg gcggccccga tcccgagttg gccagcgctg ttgtcgcccc agcagaccac
     1741 gctgccatcc gcgagcgccg cgcaggtatg ggccgccccg gcatcgatcg cgacgacgac
     1801 cgtgtcccaa tcgagggcca acggggtaag cctttcgccg agttcgaagg gagcgcctcc
     1861 gcgatgccgg gctccgctga cgccgagttg cccggactcg ttcgcgcccc agcacttcac
     1921 cgtgccgtca tcgagaaggg cgcaggtgtg acgatcccca gccgtcagcg aaaccgccgt
     1981 tcgcgccgcg ccaaggtcga ccgggcgcag cctcgccatc tcaccgggtt catcgccgcg
     2041 ccgttctacg tccccctgcc ccaactgacc ggcggcgttt tcgccccaac atttcacgac
     2101 gccgccttcg agcaaggcgc acgtatgtgc accgccggtg acgacgctca gcgcgggtga
     2161 gccgagctcg actgcctcga gcggcagatc ggaagggtct ccgcgacgac gtagccggtc
     2221 gcccacaccg agctgtccgg cggagttttc cccccaacag aagacacgcc cgctcgcgag
     2281 cttcgcgcag gcatgggcgc ccccggcggc gacctgaagc tttggcgtgc actcggcgtt
     2341 gacacaactg ttcgccccag ctgggcagcg cgaaggctcg ccgaagttgc cggtttcgtc
     2401 gcatacggct cggctcgttt cgtcgatgca gcgcgcggag cccggcgtgc agcgaaggca
     2461 ctgaccggcg gcacacacgg gcgtctcgtc tccgcacgcc tgaacctgcg accaagtcac
     2521 gccgtccatg tcgcagcgct cgacggccgc tcgggaagcg cagcgcgtgg tcccgggaac
     2581 gcactcgact ggtggtgggg ccgcgtcggt ctcgagtcga gaacactggc gaccatggct
     2641 cgtgcacctc ccgttcgagc agtccatgcc gtcggggcat tggttgccat cgccacagag
     2701 gacggcgcac tcgcggcgcg tcggccggta gcaagcgaac acagtgaagc ccaaaacgag
     2761 tagggccgcg cggaagagcg acacgcgcac cgcgctcgtc atcggacgcg tgccatgtcg
     2821 aaatacgtgt cgtgagtcgt gcctcgaacc gggttctcga tgatgtccat gaacccggct
     2881 atgatgtcgt cgcgcggggg atcggcgtaa taggcgttgc cggggaacgc agtgttcgag
     2941 tcgtcgctga gttgccagcg tttgcggatc acccagccag gctgcggccc ctcgaaatca
     3001 tggaatacgg cataagagac cccgagatcc ggaaactcgg acatggtgtc gtccgcgctc
     3061 ggagcgttgc gaaaatagca gaagacgagg cgcgtgaagt taccgagcgc ccgatcgacg
     3121 atctcgttcg gatacaggag ctctggggtg ttcgtcggag cgttttgagt gtcctggtcc
     3181 tcgttcacaa tcaccttcgt gacctccacc gcatgcttcg ggcaagagtc accgaacttg
     3241 agcaccgcat aaaactgagc gcggtcgagc gggtccgtcg tgagcggctt gaatgcgtcg
     3301 gccggtaccg agcagcgaca caagcgaat
//
LOCUS       DASTKS010001296         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1996723, whole genome shotgun sequence.
ACCESSION   DASTKS010001296 DASTKS010000000
VERSION     DASTKS010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996723"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFQ35_27790"
     CDS             <1..472
                     /locus_tag="VFQ35_27790"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET9934550.1"
                     /translation="NQFRRLFGLKPLESIDQLTDDPEALRALKSVYASIEDVDLLVGN
                     LAESHRPTGFGFGETLFEVFILNASRRLQADRFFTDSYREDIYTAEGLAWVDDATLKK
                     VILRHFPELAKTGLASVDNAFEPWDSGPLDPKRHPLRQFDDGLRDLDEKALLQS"
     gene            482..949
                     /locus_tag="VFQ35_27795"
     CDS             482..949
                     /locus_tag="VFQ35_27795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934551.1"
                     /translation="MGGDSNPFDHYTLDFNLAGGGTAVELGIDEAAATLANALTTGVT
                     VKAGLDNIAIKELKASLEAKITQLVPIDLKITQLPKIVTDSKVDLGLDNIRIAELPPV
                     QLELAVRPVRVHLPLNYTFSLELFGYRLFKFSICGEGMVVTEDYKPHATERCE"
     gene            962..1759
                     /locus_tag="VFQ35_27800"
     CDS             962..1759
                     /locus_tag="VFQ35_27800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934552.1"
                     /translation="MAKLVEQSRLSFGEAPIVFVGEPRRCVGVVEIHNPHDEPVKMRR
                     LRLQSTEPKLQNGCEPDVIDANVIARLCACETKRVEVSLAFPPTTPPGHYRAWLGGLE
                     GARCPVSIRLLELRSLRLSPGRVSHTTSPGATFETCLTVTNLGNVPLTIPHGTPLVFR
                     DPRRGWHQHFHASVATHGEQGNQQFLDDFLKRIAAAEPPVGRTKIKAGSGVVAPAESR
                     VLEIEVCMPKKLHAPCNYVAFANLGDATLTLALHVEKPVEPPEPPIG"
     gene            1762..>2205
                     /locus_tag="VFQ35_27805"
     CDS             1762..>2205
                     /locus_tag="VFQ35_27805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934553.1"
                     /translation="MLKHLLGKWATAVAGACQHESGHRHPKCCDECQVGETACPPRCV
                     GHVDWKLERGATAYATLEVRNVGSAARTFKFAGTALAGPQPGTAALAVTPASAVLQPG
                     ERTVVQVQLNGSIALQPLQSYFAEVLITGAWEQAVQVAVHVAADPF"
BASE COUNT          402 a          720 c          706 g          377 t
ORIGIN      
        1 caaccagttc cgccggttgt tcgggctcaa gccgctcgaa agcatcgatc agctgacgga
       61 cgatcccgaa gcgctgcgcg cgctcaagtc ggtgtacgcg agcatcgaag acgtcgactt
      121 gctcgtcggc aacctcgccg agtcgcatcg cccgacgggc ttcggcttcg gggagacgct
      181 cttcgaggtc ttcattctca acgcgtcgcg gcgcttgcag gccgatcgct tcttcacgga
      241 ttcgtaccgc gaggatatct acaccgcgga agggctcgcc tgggtcgacg acgccacgct
      301 gaagaaggtg atcctccgcc acttcccgga gctcgcgaag acgggcctcg cgagcgtcga
      361 caacgcgttc gagccgtggg attcgggccc gctcgatccg aagcgccatc cgctccggca
      421 gttcgacgac ggattgcgcg atctcgatga aaaggcgctg ctccagtcct gagtcaaagc
      481 catgggcggc gacagcaatc ccttcgacca ctacacgctc gatttcaacc tcgccggcgg
      541 tggcaccgcc gtcgagctag gaatcgacga ggcggcggcg acgctcgcca acgctttgac
      601 gacgggtgtg acagtcaagg ccggcctcga caacattgcg atcaaagagc tcaaagcttc
      661 gctcgaagcg aagatcacgc agctcgttcc gatcgacctg aagatcaccc agcttcccaa
      721 gatcgtcacg gattcgaagg tcgatttggg cctcgacaac atccgcatcg ccgagctccc
      781 gcccgttcag ctggagctcg cggtgcggcc ggttcgagtc cacttgccgc tgaactacac
      841 gttcagtctc gagctgttcg gctatcgact gttcaagttc tcgatctgtg gcgagggcat
      901 ggtcgtcacg gaagactaca aaccgcacgc gaccgagcgc tgcgagtagc gcgagtagct
      961 catggcgaag ctcgtcgagc agagtcgctt gtcgttcggg gaggcgccga tcgtcttcgt
     1021 cggggagccc cggcgctgcg tcggcgtcgt cgaaatccac aatccgcacg acgagccggt
     1081 gaagatgcgc cgcctgcggc tgcagagcac agagccgaag ctgcaaaacg gctgcgagcc
     1141 ggacgtcatc gacgcgaacg tcatcgcgcg gctgtgcgcg tgcgagacca aacgcgtcga
     1201 agtcagcttg gcatttccgc ccacgacgcc gccaggtcac tatcgagcgt ggctgggcgg
     1261 cctcgaaggt gcgcgctgtc cggtgtcgat tcgactcttg gagttgcgga gtcttcgact
     1321 gtcacccggc cgcgtgagtc atacgacgtc gcccggcgcg acgttcgaga cgtgtctcac
     1381 ggtcacgaac ctcggaaacg tgccgctgac gattccacac ggcacgccgc tcgtgtttcg
     1441 ggatcctcgg cgcggctggc accaacactt ccacgcctcg gtggcgacgc atggtgagca
     1501 gggaaaccag cagttcctcg acgacttcct gaagcggatt gcggcggccg agcctccggt
     1561 cggccggacg aagatcaaag ccggttcggg cgtcgtcgcg ccggccgaaa gtcgcgtgct
     1621 cgagatcgaa gtctgcatgc cgaagaagct ccacgcgccg tgcaactacg tcgcgtttgc
     1681 caatctcggt gacgcgacgc tcacactcgc gcttcacgtg gaaaagcccg tggaaccacc
     1741 cgagccgccg ataggatgac tatgttgaaa caccttctcg gaaaatgggc gaccgcggtc
     1801 gccggtgcgt gccagcacga gtccgggcat cgtcacccga agtgctgtga cgagtgtcag
     1861 gtcggggaga ctgcgtgccc gcctcgctgc gtcgggcacg tcgattggaa gctcgagcgc
     1921 ggcgcgacgg cgtacgcaac gctcgaggtt cgcaacgtcg gcagcgcagc gcgcacgttc
     1981 aagttcgcgg ggacggcgct cgccggccca cagccgggca cggcggccct cgcagtgaca
     2041 cctgccagtg cagtgttgca gccgggcgaa cgcaccgtcg tacaggtcca gctcaacggc
     2101 tcgatcgcct tgcagccgct ccagagttac ttcgccgaag tgctgatcac gggcgcttgg
     2161 gagcaagcgg tccaggtcgc ggtgcacgtc gccgccgatc cgttc
//
LOCUS       DASTKS010001297         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_877756, whole genome shotgun sequence.
ACCESSION   DASTKS010001297 DASTKS010000000
VERSION     DASTKS010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877756"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..924)
                     /locus_tag="VFQ35_27810"
     CDS             complement(16..924)
                     /locus_tag="VFQ35_27810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934554.1"
                     /translation="MRETSEPEALGPFASVVGALGAGVIYLLMRLFGRTLREAEVPWL
                     VGPTGGDYIGDKPYEEVAEREGLVVQRRAAQGGLVPDFQVLQSAAFDVAKLQPAVRDF
                     YENTASYRMDVWSESFFPGKIGLWLLVTTLSRKVNQLNFPLSALSTAKGLDSEIVLLK
                     DAAGAVRYAGWYRRLVSSGRVIYTGFYMTEALPHCVGRCVKVVFPMPSGNATVVLRPS
                     LDPDGNLVLSSKGARLGDAGFYRVRKTGVGRLLVWRISTLHEHFRVYVENETVRCDHR
                     VSFLGLPILHLHYRIERARAPSSSAA"
     gene            complement(997..2403)
                     /locus_tag="VFQ35_27815"
     CDS             complement(997..2403)
                     /locus_tag="VFQ35_27815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9934555.1"
                     /translation="MKKLLVTAMCAVAFAGCVAKDAGYGDVRSLTAERIGKDVRWRAH
                     ESAAKGEDVTRSLLSRPLTADSAVQIGLLNSARVQAAFSELGLARAELVRALAIPNPT
                     AEVAVRFRDGERPDIEISAFEDLMGLVLLPTRQGAARASLDAAKLETAGRILDEALSI
                     RLAFYDYQASAQTLALRQTILQAVRASFDAARSMHEAGNVTDLSLANERALYEEARVE
                     FSRAEANERALREQLNDRMGLFTLGSTWTVEPRLTDAAPATPILAELEQRALEKSLDL
                     KAARARYAAAAGRANYARARGVLPVLRAGVSAERAEGSSFAVGPAAVVGVPLFYQGQG
                     DIDAATAQMQEQRQLYDSQALRIRTVARSLSARLESAAASADYYKRVLLPLRSEIVQN
                     TELEYNAMSVGIFQLLQSKRDQIRAAESYVDMLREYWTLRSQAEQLRAGRLPQSAVVD
                     TPSNVAAMPAAESGGAGH"
     gene            complement(2400..>2719)
                     /locus_tag="VFQ35_27820"
     CDS             complement(2400..>2719)
                     /locus_tag="VFQ35_27820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9934556.1"
                     /translation="TLICIGILAAACTSTTFNEGEASVGNPKARTVAMREPTRVFDAN
                     FDPSPSSERTDVNPHDHGAEAAAAYTCPMHPEIVRSEPGSCPICGMKLVPKAPSPKHE
                     GTH"
BASE COUNT          477 a          901 c          875 g          466 t
ORIGIN      
        1 aacttgggac accccctagg ccgccgaact cgagggagct cgcgctcgct cgatgcgata
       61 gtgcaagtgc agaatcggga gcccgagaaa gctgacccga tggtcgcagc gcaccgtttc
      121 gttttcgacg taaacgcgaa agtgctcgtg cagcgtgctg attcgccaaa ccaacaggcg
      181 accaacgccc gtcttacgca ctcgataaaa gccggcgtcg cccaggcgag ctcctttact
      241 gctgagaacg aggttgccgt ccgggtccaa cgacggacgg aggacgacgg tggcgttgcc
      301 gctcggcatc gggaacacga ccttgacgca ccgcccgaca cagtgcggga gcgcctcggt
      361 catgtaaaag ccggtgtaga tgacgcgacc gctgctcacc aatcggcggt accagccggc
      421 gtaccgcacc gcgcctgctg cgtctttcag gagcacgatc tcgctatcga ggcctttggc
      481 cgtggagagc gcgctgagcg gaaagttcag ttgattgacc ttgcggctca gtgtggtcac
      541 gagcagccag agcccaatct ttccgggaaa gaacgactcc gaccaaacgt ccatgcgata
      601 gctcgccgtg ttttcgtaaa agtcgcgcac cgctggttgc agcttcgcga cgtcgaacgc
      661 ggcgctctga aggacctgga aatcggggac caagccgccc tgcgccgcac gccgctgcac
      721 gacgagccct tcgcgctccg ccacctcttc gtaaggcttg tctccgatgt agtcgcctcc
      781 ggtcggtccc accaaccagg gcacctccgc ctcgcgcagc gtccgcccga agaggcgcat
      841 cagcaaatag atgacgccgg ccccgagcgc gccaacgacc gacgcaaaag gccccaaagc
      901 ttccggttcg ctcgtctccc tcacgcgcag aaccttagcg catttcgcgc cgctctacgt
      961 cacgctcgag cagcgcggag aagttggggc tcgtagtcaa tgcccagcgc caccgctctc
     1021 ggcggcgggc atggccgcga cgttggacgg cgtgtcgacc accgcgctct gggggaggcg
     1081 gcccgcgcga agctgctccg cttgcgagcg taaagtccaa tattcacgaa gcatatcgac
     1141 atacgactcc gccgcgcgga tctgatcgcg cttcgactgg agcaattgaa agatgccgac
     1201 gctcatcgcg ttgtactcga gctccgtatt ctggacgatt tcgctgcgaa gtggcaggag
     1261 cacccgcttg tagtagtcgg cgctcgcggc cgccgactcg agccgagcgc tcaggcttcg
     1321 cgcgacggtg cgaatgcgaa gggcttggct gtcatagagc tgccgctgct cttgcatttg
     1381 cgcagtcgcg gcgtcgatgt cgccctgccc ctgatagaag agcggcacgc cgacgaccgc
     1441 agccgggccc acggcaaagg agcttccttc tgcgcgctcc gcggagacgc ccgcgcgcag
     1501 caccggtaag acaccgcgcg cccgcgcgta gttcgcacga cccgcggcgg ccgcgtaccg
     1561 cgcgcgcgcc gccttcaaat cgaggctctt ttcgagagct cgctgctcga gctcggcgag
     1621 aatgggggtc gcgggagcgg cgtcggtcag tcgcggctcg accgtccaag tgcttcctag
     1681 cgtgaagagg cccatgcggt cgttcagctg ctcgcgaagc gctcgctcgt tggcttcggc
     1741 gcgggagaac tcgacccggg cctcttcgta gagggcgcgc tcgttcgcga ggctcaaatc
     1801 ggtgacgttg cccgcctcgt gcatgctgcg cgcggcatcg aacgaagcgc gcacggcttg
     1861 caagatggtc tgacgcagcg cgagcgtctg ggcgctcgcc tggtagtcgt aaaacgcgag
     1921 gcgaatcgag agggcctcgt ccagaatgcg cccggcggtc tcgagcttgg cggcgtcgag
     1981 cgacgctcgc gcggctcctt ggcgggtcgg cagtaacacg agccccatca agtcttcgaa
     2041 cgcgctgatc tcgatgtccg ggcgctcgcc gtcgcgaaac cgcaccgcga cctcggcggt
     2101 aggatttgga atcgcaagtg cccgcacgag ctctgcgcga gccaagccga gctcggagaa
     2161 cgccgcctga acgcgcgcgc tgttcaagag cccgatctgg accgcggagt cagcggtgag
     2221 cggacgggac aagagcgagc gcgtgacgtc ttcacccttt gcggcgctct cgtgggcacg
     2281 ccaacgcacg tctttaccga tgcgttcggc ggtgaggctc ctcacgtcgc cgtagcccgc
     2341 atcctttgcg acgcacccgg cgaacgcgac cgcgcacatc gcggtaacga gtaacttttt
     2401 caatgcgttc cctcgtgctt cggactgggc gccttgggga cgagcttcat cccacagatg
     2461 ggacaactgc caggctcact gcgcacgatt tcgggatgca tcgggcaggt gtacgcggcc
     2521 gccgcctcgg cgccgtgatc gtgcggattc acgtccgttc gctcggagct cggcgaaggg
     2581 tcgaagttcg cgtcgaagac gcgcgttggt tcgcgcatcg caaccgtccg ggcctttgga
     2641 tttccgacgc tggcttcgcc ctcgttgaag gtcgtcgagg tacatgccgc ggccaaaatc
     2701 ccaatgcaaa tgagcgtcg
//
LOCUS       DASTKS010001298         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_209421, whole genome shotgun sequence.
ACCESSION   DASTKS010001298 DASTKS010000000
VERSION     DASTKS010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209421"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFQ35_27825"
     CDS             <1..252
                     /locus_tag="VFQ35_27825"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934557.1"
                     /translation="SGLHRELRDDRVLTFLSHVDPGIYHFRYLARATTPGRYVVPPLR
                     AECMYSPEVSGRTAATTFEVVGDTPKPSVAKVAALSRTP"
     gene            249..>2610
                     /gene="pbpC"
                     /locus_tag="VFQ35_27830"
     CDS             249..>2610
                     /gene="pbpC"
                     /locus_tag="VFQ35_27830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HET9934558.1"
                     /translation="MSRKRRAAWGLAAAFAVAAAWVAATPLPEELRRSHAEPSLRVVD
                     TNERLLRDVRASDGARARFASLAELGPNAKNAILAAEDRRFFAHPGVDPLALTRAVLG
                     AVRHLRVVSGGSTITMQLARTLRPHPRTVFGKVREMVLALRIEASLSKSQILQEYLNR
                     VSFGPNLRGYAAASHAYFGKTPGNLSVAEAAFIAGLPQSPSRYSLERNRRAAEARRNR
                     VIARMLEMSVIDAELAKRAQAEPLRPGSLRPAFGAPHLVQGLVNGGLWSFQPGLREVL
                     RKPASQLTTTLDATLQVEAETAVSTALSALSAKQATAGSVVVLDNSSGDVLAYVGSPD
                     FFDEARLGQNDGARALRQPGSTLKPFLYALAMEKLGFTAATALPDVESHFATKGGRFS
                     PRNYDSKVRGPVRLREALGNSLNIPAVWTLSQLGVEPFFARLRELGFDSLVEAPDYYG
                     LALALGDGEVRLLDLAAAYATLARGGVYKPSRLVRGVERVGEPPVELAPAEGRRVIPA
                     LIAAQLTDILSDPRARSAAFGAHTLLDFPFPVAAKTGTSKGFRDNWVIGYTHAVTVAV
                     WVGNFDGGAMENVSGIAGAGPIFHSVMEAAMRRSNARDASSPLTLSEGAPREGLHRVE
                     ICSLSGGLASVDCPHKIREWVADDAVLSSCDMHERVRLNRQDGLRAGSTCARDEVVER
                     TFERFPPEYLAWATAASRPLAPRDFSPMCPDAPHPVEPREAAIRILYPADGARFAIDP
                     ERPASVQLLSVPLAVPEGTREAALLVDGEVVDTVHAPFVAQWLLVPG"
BASE COUNT          406 a          931 c          839 g          434 t
ORIGIN      
        1 agcggcttgc atcgtgagct gcgcgatgac cgcgtactca ccttcctttc gcacgtcgat
       61 ccgggcatct accacttccg ctacctagcc cgggcgacga cgccggggcg ctacgtcgtg
      121 ccgccgctcc gcgccgagtg catgtattcg ccggaggtca gcgggcgcac tgccgccacg
      181 accttcgaag tcgtgggcga cacgcccaag ccttcggtcg cgaaggtggc ggcgctctcg
      241 agaacgccat gagccgaaag cgccgtgcag cgtggggtct cgccgcggcg ttcgccgtcg
      301 ccgcggcgtg ggtagccgcg acaccgctgc cggaagagct ccgccgtagt cacgccgagc
      361 cctcgctccg cgtcgtcgac acgaacgagc ggctcttgcg tgacgtgcgg gcgagcgacg
      421 gcgcccgcgc gcggttcgcg tccctggccg agctcggacc caacgcgaag aacgcgatcc
      481 tcgccgccga ggatcgacgc ttcttcgcgc acccgggcgt cgatccgctc gcgttgacgc
      541 gcgcggtgct cggcgcggta cgccatctcc gagtcgtctc cggggggtcc accatcacga
      601 tgcagctcgc gcgcacgctg cgcccccacc cgcggaccgt gttcggaaag gtccgcgaga
      661 tggtgctagc gctgcgcatc gaggcttctc tctcgaagtc gcaaatcctc caagagtacc
      721 tgaaccgcgt gagcttcggg cccaacctga gagggtatgc agcggcgagc catgcgtact
      781 tcgggaaaac gccaggcaac ctctccgtgg ccgaagcagc gttcatcgcc gggctacccc
      841 agagcccttc gcgctactcg ctcgaacgga accgtcgtgc cgccgaagcg cgacgcaacc
      901 gcgtcatcgc tcgaatgctc gagatgagcg tcatcgacgc cgaactcgcg aagcgcgcgc
      961 aggcagagcc cttgcgtccc ggctcgctgc ggccggcatt cggggctccg cacctagtgc
     1021 aaggactggt caacggtggt ctctggtcct tccaaccggg gcttcgcgag gtcttgcgca
     1081 aacccgcttc gcagctcacg acgacgctcg acgctaccct gcaagtcgaa gcagaaaccg
     1141 cggtttcaac ggcgttgagc gcgctcagcg ccaagcaagc caccgccggc agcgtcgtcg
     1201 tgctcgacaa ctcgagcggc gacgtgctcg cttacgtcgg ctcccccgac ttcttcgacg
     1261 aagcgcgact cggccagaac gacggcgcgc gcgccctgcg tcaaccgggc tcgacgttga
     1321 agcctttcct ctacgcgctc gcgatggaga agctggggtt cacggccgcg acagcgctcc
     1381 ccgacgtcga aagccacttc gccacgaaag gcggtagatt ctccccccgc aactacgaca
     1441 gcaaggtgcg cggccccgtg cgcctgcgcg aagccctcgg caattcgctg aacattcctg
     1501 ctgtgtggac gctgagtcag ctcggcgtcg agcctttttt tgctcggctg cgcgaactcg
     1561 gcttcgactc gctcgtcgaa gcaccggact attacgggct cgcgctcgcg ctgggtgacg
     1621 gtgaggttcg gctcctcgat ctcgcggcgg catacgcgac gctcgcgcgg ggcggggtgt
     1681 acaaaccctc gcgcctggtt cgcggggtcg agcgcgttgg cgagccgccg gtcgaactcg
     1741 ccccggcgga aggacggcga gtaattccag cgctgatcgc ggctcagctc accgacatcc
     1801 tcagcgatcc ccgagcgcgc agcgcagctt tcggagcaca cacactgctc gacttccctt
     1861 tcccagttgc ggccaagacc ggcacctcca agggctttcg cgacaactgg gtcatcggct
     1921 acacgcacgc cgtaacggtc gcggtctggg tcggtaactt cgacgggggc gcgatggaga
     1981 acgtttctgg gatcgccggg gcaggaccca tcttccactc ggtgatggaa gctgcgatgc
     2041 gccgctccaa cgcgcgcgac gcttcgtctc ctctcacgct ctccgaggga gctcctcgtg
     2101 aagggctgca ccgcgtcgaa atttgctcgc tctccggggg cctcgcgagc gtcgactgcc
     2161 ctcacaagat ccgcgaatgg gtcgctgacg acgccgtgct ctcgagctgc gacatgcacg
     2221 agcgcgtccg gctgaatcgg caagatgggc ttcgcgctgg gtccacgtgc gcgcgggacg
     2281 aagtcgtcga gcgcaccttc gagcgatttc ctccagagta cctggcgtgg gcgaccgcag
     2341 catcccggcc cttggcgccc cgcgacttct cgccgatgtg tccggacgcg ccgcaccctg
     2401 tagaaccccg cgaagccgcg atccgcatct tgtatcccgc tgacggcgcg cgcttcgcga
     2461 tcgaccccga gcgaccggcc tcggtgcagc tcctcagtgt gccgctcgct gtccccgaag
     2521 gcactcggga agccgcgttg ctcgtcgacg gagaagtcgt ggatacggtg cacgcgccct
     2581 tcgtcgcgca atggctcctc gtcccgggag
//
LOCUS       DASTKS010001299         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1997452, whole genome shotgun sequence.
ACCESSION   DASTKS010001299 DASTKS010000000
VERSION     DASTKS010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997452"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VFQ35_27835"
     CDS             complement(<1..1626)
                     /locus_tag="VFQ35_27835"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9934559.1"
                     /translation="MPAVERPDVEDPRDTPIFEFSGLRVDTRRRELTRHGKSIGLYPR
                     AFDALVFLVTHRRAVVTKARLLDALWPGLAVEENSVARVISDLRKALGPAAGSIATVQ
                     GLGYRFDAAVHVLPRSVQRSSEPAKRILAILPFSSVGEDHEQLGFGLADALITRLSRL
                     RNVVVRSTSSVVRFAAANVTPAEAGTALRADLVLCGSLRRHDQMIRISVQLIDVENEA
                     SVWAEQFDESMSDVFAMEDAIATRVTPLLVSELARPETELALRGTDNADAYVLAQRGR
                     YWLTRRQAPESASKAVQCFEAAIALDSRFAGAHAGLANAYIAGAIAAFTTWVQPPRTM
                     IPLARAAAEQALRLVPDLGEAHGVLAHIAFGYDWNWPAAKAAFRHALQLDPRNVLCRQ
                     SFAIGLCCAGEFDAALHQLEVAREFDPGSMLVRTNVGFVFHSWRRPDQAIAELEACLA
                     LEPAFAYARYRYGLALEASGRHEDALAQFERLRNTPGAEVQGLAGSACGFARTGRVAD
                     ANACVAELLLLSERRYVSAYFLAEIFASLGDVAE"
     gene            complement(1654..2181)
                     /locus_tag="VFQ35_27840"
     CDS             complement(1654..2181)
                     /locus_tag="VFQ35_27840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET9934560.1"
                     /translation="MCRPSVWTSYPPQERCPRNRIKSSARKYVLDTQLFINAFRDPIA
                     NETLQRFHRAFAPFEYLSVIVAQELRAGVGKPSDRKALERNVLRVFERAGRTITPSPA
                     AWHRSGDLFSEMARKEGLEVARVSKAFANDLMLALSCRESGCVLVTENDHDFARIRRF
                     VSFEYVKPWPARLHP"
     gene            2169..2474
                     /locus_tag="VFQ35_27845"
     CDS             2169..2474
                     /locus_tag="VFQ35_27845"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9934561.1"
                     /translation="MADTPKRRARRQFTDEFKASAVRLVLDEGKSVGAAARDLDLTET
                     ALREWVKRAQADRTKGRTGLTTAEREELVRLRKALRIAEEERDILKKATAFFAKQSR"
BASE COUNT          487 a          781 c          828 g          415 t
ORIGIN      
        1 ctcggcgacg tcgccaagcg acgcgaagat ctcggcgagg aagtacgccg agacgtaccg
       61 gcgttcggaa aggaggagca gttcggcaac gcacgcgttc gcgtccgcga ctcggccggt
      121 tcgcgcgaac ccgcaggcgg atcctgccag tccctgcact tcggctcctg gcgtgtttct
      181 cagccgctcg aattgagcga gggcatcctc gtgacggccg ctcgcttcaa gggcgaggcc
      241 gtagcgatac cgcgcgtacg caaaggccgg ctccagcgcc aggcaggctt ccagctccgc
      301 gatcgcctga tccggtcgcc gccaggaatg aaagacaaag ccaacgttgg ttcgcaccag
      361 catcgaaccc gggtcgaatt cgcgcgccac ttccagttga tgaagggcgg cgtcgaactc
      421 gccggcgcaa cagagtccga tggcgaagga ttggcgacag agcacgttcc gcggatcgag
      481 ctggagtgcg tggcggaacg cagccttggc ggccggccag ttccaatcgt agccaaatgc
      541 gatatgcgcg agcacgccgt gcgcttcgcc gaggtcaggt acgaggcgca atgcctgctc
      601 cgccgctgcg cgggcgagcg ggatcattgt tcgaggcggc tgcacccacg tcgtaaacgc
      661 ggcgatggcg ccggcgatgt aggcgttggc caagcctgcg tgcgctccgg caaagcgcga
      721 gtcgagggca atcgcggcct cgaagcactg gacggcttta cttgccgact caggcgcctg
      781 acgcctcgtc aaccaatatc ggcctcgctg cgccagcacg tacgcgtccg cgttgtcggt
      841 tccccgtagt gcgagctcgg tttcaggtcg ggcgagctcg ctgaccagaa ggggcgtgac
      901 acgcgtcgca atcgcgtcct ccatcgcaaa gacatccgac atggactcat cgaactgttc
      961 cgcccacacc gatgcttcgt tctcgacgtc gatcaattgg actgagattc ggatcatctg
     1021 atcatgacgt cgcagcgagc cgcagaggac gaggtcggcg cgcagcgccg ttccggcctc
     1081 tgccggggtg acgttggcgg cggcgaaacg caccactgag ctggtcgagc gaacgaccac
     1141 gttgcgcaac ctgctcagtc gggtgatgag tgcgtcggca agtccgaatc ccagttgctc
     1201 gtggtcttca ccgacactgg agaacggcaa aatcgcgagg atccgtttgg cgggctcact
     1261 cgaccgctga acgctgcgag ggaggacgtg aacggcggcg tcgaatcggt agccgagtcc
     1321 ctgtaccgtg gcgatactgc ctgcggccgg accgagcgct tttcgcaggt ccgagatgac
     1381 cctcgccacg ctgttctcct caacggcgag tcccggccac agcgcgtcga gcaacctcgc
     1441 cttcgtgacg accgcgcgcc tgtgcgtgac aaggaaaacg agcgcgtcga acgcgcgcgg
     1501 atagagcccg atgctcttcc cgtgacgtgt cagctctcgt cgacgagtat cgacgcgtag
     1561 acctgagaac tcgaagatcg gtgtatcacg cgggtcctcg acatccggac gctcgactgc
     1621 aggcatgggc gatgttcgac ggcgtcgaag atactacgga tgcagacgcg cgggccacgg
     1681 cttcacatac tcgaatgaga cgaaccggcg aatgcgagca aaatcatgat cgttttcggt
     1741 gacgagcaca caaccggact cgcggcacga aagtgcgagc atgagatcgt tcgcgaaggc
     1801 cttggaaact cgagcgacct ctaggccttc cttccgcgcc atttcggaga acagatcgcc
     1861 cgagcgatgc caagcggctg gtgacggcgt gatggtccga cccgctcgct cgaacaccct
     1921 caggacattt cgttccaacg ccttccgatc gctcggcttg ccgacgccgg cgcgcagttc
     1981 ctgagcaacg atgacgctga ggtactcgaa tggcgcgaac gcgcgatgaa aacgctggag
     2041 cgtctcgttc gcgatggggt cgcgaaaggc gttgataaag agctgcgtat cgaggacgta
     2101 cttgcgggcg ctggacttga tccggtttcg tggacatctt tcctgtgggg gatacgatgt
     2161 ccaaaccgat ggccgacaca ccgaagcggc gagcgcgccg ccagttcact gatgagttca
     2221 aagccagcgc cgtgcgcctg gtcctcgacg aaggcaagag cgtcggggcg gcggcgcgcg
     2281 atctcgatct gaccgagacc gcgttgcgcg agtgggtcaa gcgcgcgcag gcggaccgca
     2341 ccaagggccg cacgggcctg acgaccgccg agcgcgagga gctggtccgg ctgcgcaaag
     2401 cgctccgcat cgcggaggaa gagcgagaca tcttaaaaaa agccacggcg ttcttcgcga
     2461 agcagagccg gtgaggtttg ccttcatcgc ggcgaagaag gccgagcatc g
//
LOCUS       DASTKS010001300         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_209617, whole genome shotgun sequence.
ACCESSION   DASTKS010001300 DASTKS010000000
VERSION     DASTKS010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209617"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFQ35_27850"
     CDS             <1..1063
                     /locus_tag="VFQ35_27850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934562.1"
                     /translation="RDVTVECWAPERRARRRATLEHSGVPYSLFPSLALGYEREVSPS
                     LLTHFEREARTIDAVFLHGSVSYLSALLLRRFGHVVPIIVQNHGETSTLARAQRYGRK
                     KPLDYSLRVRLERRAFSKASRILCLNPENIEDYVLNGVAPERLMLSAMGVDLDAFGPL
                     AESRQTLRAALGLPANAVCLAFVGRLAQEKRVDRIFALMQRLPSDHHLLVIGGGPEDR
                     RLRARGAVFGDRVHFIGEITDRVRLNRYYNASDLLVLASEREGLGMVLLESLAAGVPV
                     VASNLPGPRSVLEAPSCGLSCDTEDPAAFAGAVQRALSLPRDLSARRARAARYSWQSV
                     CDNHAAILESLPRRRGSTA"
     gene            1060..2244
                     /locus_tag="VFQ35_27855"
     CDS             1060..2244
                     /locus_tag="VFQ35_27855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9934563.1"
                     /translation="MNIALLCPYLPAPAHSGGRIRIHRLAAALARLGEVSLFASAGQS
                     DLDQHRNDPGLSLFTRTHLAHTAFSWLSSNGVPARVRRCASTSLKHALLRSHAERPFS
                     VVVVEHAQAASLACDLGVPWVLDEHNVESEYLRERLRAQGRLVEVVARREIAALQRWE
                     QRCWRAATRVTCVSEADAAEIARQSGRRPTVIPNGISLREIRFILPSHRRGYEIAFIG
                     LMGHAPNAAAARFLAREVLPRVQRVEPRARLTLCGADPTAAVRALAGPDVEVTGRVAS
                     VNPYLERAAVYANPLQHGAGTSLKVLEALAAGTPLVSSESGVRGFALEAPRHFLAAES
                     AEQFAGQILSCFRDRGSRDGAARAGRNFAEAHDFATIGESFAAVIVDAVRTRGPDQRE
                     AT"
     gene            2241..3716
                     /locus_tag="VFQ35_27860"
     CDS             2241..3716
                     /locus_tag="VFQ35_27860"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9934564.1"
                     /translation="MNAPGVSLDRTHEVGRHVVRQTTAYALLRSTSGIITWAAFVLLT
                     RILDRRSFGAFEIGMFYVGVGQIIGDGGLAASLVRRRGRVLPIQYRTAQTAAFALAAT
                     LALALNACASFIGRANHLTPSEVWALRALAPLYLVPAFRVVPYAKLERRVDFAAIGRV
                     ELYANLARHTTAVLVALLEGGVWALVLSQLVLAVTQLVLAYRAAPGLAGFAFSRRAFR
                     SLYGYGSKIQASVLLLHFKDNLSAGLLGPALGPTAVGVFRFALEFVRVPADIGSSLAR
                     VQFPVYTQHRQHSTELRDVIRGALRGSFIVGLPALGVFALLSEWVIPLVYGHKWAAAL
                     PLVIALVPHVAADLLIFHLVTFVQGRGRAGLALGVYAAWSLSLWLFSAGALEAAPGSL
                     SAVALAHGAGSLLIVAGLLWWVSRYLEYSLWRALAAPFCAAALSFVATLALRSTWTTS
                     NVVEAAGSALFFVALYLACLLSVEGRRVVSEVRSALQSFRP"
     gene            complement(3720..>4329)
                     /locus_tag="VFQ35_27865"
     CDS             complement(3720..>4329)
                     /locus_tag="VFQ35_27865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934565.1"
                     /translation="EASIPAPPKVGLVARTGVDFAKDAAAAPLDKIVAEHTRGNAAVS
                     STGELRHDPSAGRFEVDTPRTQGFAGFRSDAPIALGNVRIESKTPFAAVVVTALDDAP
                     IAASKHWLVTAAANAVNTGMALAPSGNGLADPGHSPILVEPVEGSVGFAKLGGSLDHL
                     TAYALGASGERGSEVPLMRTRDGFILTLSPTSKTLHYEIVRK"
BASE COUNT          597 a         1514 c         1434 g          784 t
ORIGIN      
        1 ccgagacgtc acagtcgagt gctgggcccc ggagcgccga gcccggcggc gcgccacgct
       61 agagcactcg ggcgtgccat actccctgtt cccctctctc gcgctgggct atgagcgcga
      121 ggtctctccg agcctgctca cgcacttcga gcgcgaggcg cgaaccatcg acgcggtctt
      181 cctgcacggg tcggtcagct acctatccgc gctcctactt cggcgtttcg ggcatgtggt
      241 cccgatcatc gtgcagaatc acggcgagac gagcacgctc gcgcgtgccc agcgttacgg
      301 acgaaagaag ccgctggact attccctacg cgtacggctc gagcgccgcg ccttctccaa
      361 agcgtcgcgc atcctttgcc tcaacccaga aaacatagag gattacgtgc tcaacggggt
      421 cgccccagag cgactgatgc tctccgcgat gggggtggac ctcgacgcgt ttggaccgct
      481 cgccgagtcg aggcaaacgc tgcgcgccgc tctcgggttg ccggcgaacg ccgtctgcct
      541 cgcgttcgtg ggacggctgg cgcaggagaa gcgcgtcgat cgcatcttcg cgttgatgca
      601 gcgcctgcct tccgaccacc atctcttggt gatcggagga ggccccgagg atcgacggct
      661 gcgtgctcgc ggtgccgtgt tcggggaccg cgtgcatttc atcggagaga tcaccgacag
      721 ggtccgcctc aatcgctact acaacgcgag cgacctgctc gtcctcgcga gcgagcgaga
      781 aggcctcggg atggtgctct tggaatcgct ggcggccggg gttcccgtcg tcgcatcgaa
      841 cctgcctggg ccgcgcagcg tactcgaagc cccctcgtgc gggctctcgt gtgacaccga
      901 agacccggcc gcgttcgccg gcgccgtcca gcgagcgctc tctttgccac gcgacctgag
      961 cgcgcgccgt gcacgcgccg cgaggtattc ttggcaaagc gtttgcgata accacgccgc
     1021 gattctggag agcctgccgc gacgacgcgg gagcacggcg tgaacatcgc gttgctctgt
     1081 ccctaccttc cggcgcccgc tcacagcggc ggacgaattc ggattcaccg actcgcggcg
     1141 gcgctggccc gcctcggcga ggtctcgctc ttcgcgagcg ctggacagag tgatctggac
     1201 cagcaccgaa acgaccccgg gctctcgctc ttcacccgga cccacctcgc gcacacggcc
     1261 ttcagctggc tcagctcgaa cggggtgcca gcgcgcgtcc gacgttgcgc ctcgacgtcg
     1321 ttgaagcatg cgctcttgag gagccacgcc gagcgcccgt tctccgtggt cgtggtcgaa
     1381 cacgcgcagg cggcgtcgct cgcctgcgac ttgggagtgc cgtgggtcct cgacgagcac
     1441 aacgtcgagt cggagtacct tcgcgagcgg ctgcgcgccc agggtcgcct agttgaagtc
     1501 gtcgcgcgcc gcgaaatcgc cgcgctccag cgctgggagc agcgctgctg gcgcgccgcc
     1561 acgcgagtca cgtgcgtttc ggaggcggac gccgcggaga tcgcgcggca gagtggacga
     1621 cgaccgaccg tcatcccgaa cggcatctcg ctccgggaaa tacgcttcat cttgccgtcg
     1681 catcggagag gctacgagat cgcgttcatc gggctgatgg gccatgctcc gaacgccgct
     1741 gccgctcgct ttctggcgcg cgaagtactg ccgcgagttc agcgcgtgga gccgcgcgct
     1801 aggctgacgc tctgcggcgc cgacccgacc gccgcggttc gggctctcgc gggtcccgac
     1861 gtcgaggtca ccgggcgcgt cgctagcgtg aacccctacc tcgagcgcgc ggccgtgtac
     1921 gccaatccac tgcagcatgg cgccggcact tcgctgaagg tgctggaggc gctcgccgcc
     1981 ggaactccgc tcgtctcgag cgaaagcggt gtacgcggct tcgctctcga ggccccgcga
     2041 cacttccttg ccgcggagag cgccgagcag ttcgcaggcc agatcctctc ttgttttcgc
     2101 gaccgcggct cacgcgatgg cgccgcgcga gcgggccgca acttcgccga agcgcacgac
     2161 ttcgcgacaa tcggcgaaag cttcgcggcc gtcatcgtcg acgcggttcg cacgcgaggg
     2221 cccgaccaac gtgaggcgac gtgaacgcgc ccggggtcag cctcgaccgg actcacgagg
     2281 ttgggcgcca cgtagttcga caaacgaccg cgtacgcgct gctccgctcg acctccggga
     2341 taataacctg ggccgcgttc gtactactca cgcgcatctt ggaccggcgc agcttcggcg
     2401 cgttcgaaat cggcatgttc tacgtcgggg tcggccagat catcggtgac ggcggccttg
     2461 ccgcgagcct cgtccggcga cgcggccgcg tcttgccgat ccagtatcgc acggctcaga
     2521 cggcggcttt tgcgctcgcc gcaaccctcg cgctcgcgct gaacgcctgc gcgagcttca
     2581 tcggccgagc caaccacctc accccgagcg aggtttgggc gctgcgtgcg ctggcgccgc
     2641 tctacttggt ccccgcgttt cgggtggtgc cttacgcgaa gctcgagcgg agggtcgact
     2701 tcgctgcgat tggtcgtgtg gagctctatg cgaacctcgc tcgtcacacg acggcggtcc
     2761 tcgtcgccct gctcgagggt ggcgtgtggg cgctcgtgct gtcgcaactc gtcctcgcgg
     2821 ttacgcaatt ggtcctcgcg tatcgtgctg cgccggggct cgcaggtttc gcattcagtc
     2881 gacgcgcgtt ccgctccctc tacgggtacg ggagcaagat ccaggcttcc gttctcctcc
     2941 tccacttcaa ggacaacctc tcggcgggct tgctcgggcc tgcgcttggg cccaccgcgg
     3001 tcggagtctt tcgctttgcg ctcgagttcg tgcgcgtgcc ggccgacatc ggtagcagcc
     3061 tcgcccgcgt acagttcccc gtgtacacgc agcaccggca gcactcgacg gagctgcgcg
     3121 acgtgatccg cggcgcgctc cgcggatcct tcatcgtcgg cctgcccgcg ctcggcgtct
     3181 tcgcgctgct ctccgaatgg gtcattccgc tcgtctacgg tcacaagtgg gccgccgcgc
     3241 tcccgctcgt catcgcgctc gttccgcacg tggccgcaga tctcctgatc tttcacctcg
     3301 tcaccttcgt ccagggacgt ggccgtgctg gcctcgcgct cggggtctac gcggcgtggt
     3361 cgctctccct gtggttgttc agcgccggcg cgctcgaggc cgctccgggc tcgctcagcg
     3421 ccgtagctct cgctcacggc gcggggtcgt tgctgatagt ggcggggctc ctttggtggg
     3481 tgagccgcta cctcgagtac tcgctgtgga gggcgctcgc cgcgccattt tgtgcggcag
     3541 cgctatcgtt cgtcgcgacc ctcgcgctac gaagcacttg gacgacgtcg aacgtcgtcg
     3601 aggcggcggg ctcagccctc ttcttcgtcg cgctctacct cgcgtgcttg ctctccgtcg
     3661 aagggcggcg cgtggtcagc gaggtccgct cggcgctcca gtcctttcgc ccgtagcgcc
     3721 tactttctca cgatctcgta gtgcaacgtt ttgctggtag ggctcagcgt cagaatgaag
     3781 ccgtcccgag tgcgcatcaa cgggacctcc gacccgcgct cacccgaagc accgagcgca
     3841 tacgcggtga gatggtcgag ggagcctccc agcttcgcga agccgacgct cccctccacc
     3901 ggctcgacca agatcggcga atggcctgga tcggcgaggc cgttgccgct cggggcgagc
     3961 gccatgcccg tgttgacggc gttggcggcg gcggtcacga gccaatgctt cgaggcggcg
     4021 atgggagcgt cgtcgagcgc agtcactacg acggccgcga acggcgtctt cgactcgatg
     4081 cgcacgttgc cgagcgcgat cggcgcgtcg gagcggaagc cagcgaagcc ttgggtacgc
     4141 ggcgtgtcga cttcgaagcg tcccgccgag gggtcgtgcc gaagctctcc cgtgctcgag
     4201 acggcggcgt tgccgcgcgt gtgctcggcg acgattttat cgagcggtgc cgcagcggcg
     4261 tccttcgcga agtcgactcc ggtccgcgca acgaggccga ccttcggcgg cgctgggatc
     4321 gacgcttcg
//
LOCUS       DASTKS010001301         5902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_878256, whole genome shotgun sequence.
ACCESSION   DASTKS010001301 DASTKS010000000
VERSION     DASTKS010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878256"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1393
                     /locus_tag="VFQ35_27870"
     CDS             95..1393
                     /locus_tag="VFQ35_27870"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9934566.1"
                     /translation="MQRALTLFAACGVACSGPADEPNGSDELATSSQPESTVSVSPKA
                     SGISHVVVVMMENRSFDHLFGWVPGADGRQAGASYVDRAGVPHATHALAPDYQGCGHP
                     DPDHSYEGGRVEYADGACDGWLKAGSNDDFAIGYYQANDLSFFSRAVPRGTVLDRYFA
                     AIMAETYPNRIYQHAAQVDRLTNTLDVSTLPTIWDRLAAAGRSGRYYYSDVPFLALWG
                     PKYLPISRPLAGFVVDALLGTLPDVAFVDPPFLDEPSGTSSDDHPHADIRRGEAFLNL
                     LYWAVTRGRSWDNTLLVINFDEWGGFFDHVPPPTAPIPAADRAAGNEDGRLGFRTPAL
                     VIGPWARAGVVSHTVLDHTSILKLIEWRWGLPPLSERDASANNLAAVLDFSRRTPRPP
                     AFAPPLQPFGTPCTSSLGGDEVSLSSLRELAQTFGWPIYQ"
     gene            complement(1470..3149)
                     /locus_tag="VFQ35_27875"
     CDS             complement(1470..3149)
                     /locus_tag="VFQ35_27875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9934567.1"
                     /translation="MSELLGPELSAAIAKKGYLTLTPVQLSVLDPALRDRDLRVSSQT
                     GSGKTLAIGFALRDVLRDAAPASKAHLAKPAALVIVPTRELAKQVEAELSWLFAPFRL
                     KIAAVTGGASYRDEHRALASNPAIVVGTPGRLLDHLQRGGVDPSAVRAVVLDEADRML
                     DLGFRDELEAILATTPSERFTHLVSATFSPEVKRLADSIQKNPARVEGTPLGSANSDI
                     EHVVYVVRPGAQVDALVNVLLEHPNDQTLVFARTRAEVADITRELQSAGFAAGALSGE
                     MEQLARNRALAAFKQRDVRVLVATDVAARGIDVQDIARVVQIDAPTDPDTYTHRSGRT
                     GRAGRKGTSAIIIPPHALRRTLGILERARVRYRTLQVPNAEAIRAAQDARWLAELTEA
                     SRAQTEISPRIRAQVTRIIESGLAESALALLLSRSQKALGEPREIAPVALGGERRDRA
                     DFRPRSGPRSEPRADRPERNARGARDDDNGWVPFRVTWGEAHGADPRRLVAMLCRRGQ
                     IKSSDIGAIRVARTSSTVEVAAAAAPRFFEATRRPDKRDSRVVVTPVTEAR"
     gene            3396..5033
                     /locus_tag="VFQ35_27880"
     CDS             3396..5033
                     /locus_tag="VFQ35_27880"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9934568.1"
                     /translation="MSMLRSWSIGQRLIALSACLTIASIVTLFCLIKVVDKVRDLGVE
                     EAGAAAFEGEKTALKVGVDSMALTIGATIAGVSDEKQRLALITKMVTAARYGSDRAGY
                     YFVHRGTVAVVIPPAPQLTGQDRASETDKNGVFYIVELARNAGTGKFVNYQFVKPGGT
                     VASDKLGYSQLIPGTDLWIGSGVYIDNVARVQHGLGDKIVALTDLRMRPVLYVLGALY
                     LLVLPCIVLVNRSITKPLSAAVDLAKQVAEGDLRIHVEPQPKDEPGQLMSALALMGKR
                     LRDVVSQVSEDAVSLASSSSELTGASSSLAQGSNSQASSVSQVTAALEQITVSIANSA
                     DKAGQTEKLAVHASVQVNTSAAKVVESLDAMRSVGDKLSIVEEIARRTDLLALNASIE
                     AARAGSAGKGFAVVAAEVRRLAERSRDAASEIRELTQRTMLLATEAGRSMSQIVPEIE
                     RTASLVQDIAASSREIRSGAASVSDAMQRLDGVVQHNAAASEELSSTSEGLSSRAAAL
                     RSAVGFFTIDDAKSVLSIVESVQVRRAPRSLPSLTRA"
     gene            complement(5102..>5902)
                     /locus_tag="VFQ35_27885"
     CDS             complement(5102..>5902)
                     /locus_tag="VFQ35_27885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934569.1"
                     /translation="GAAESVKLSFDHARKCFAGHARAGVELAPGAFELSIRSAAGLAV
                     GGLDRIALRADAAHGGEVLVAGDYSFEVVPTGNVVRVFAFDAAGKALAKADAALKLTL
                     GATLPVNVALTWDAPSASYRGEFDGKLDLRAQPIRLRLDVDGRAFEAAAASLQAVANA
                     RLDAKAAAGAKLDVGANLPDAKLGADGKLGADAKLGADAKLGAGAKVAVPDVKANLSA
                     AAKAVGANAKVNVTPPKVNVNKSASANASTGNSGAKAKASAGFSFGIK"
BASE COUNT          954 a         1940 c         1989 g         1019 t
ORIGIN      
        1 aactccccgt gagtacgact cttgcacgac gtgagtgggt ttaatggcga gctttctcga
       61 gcgaaaatgc gggctcttcg atcgaggccg ttggatgcaa cgcgcactca cactctttgc
      121 tgcgtgcggc gttgcctgca gcggccctgc cgacgagcca aacgggagcg atgaattggc
      181 gacgtcgtcg cagcctgagt ccacggtctc ggtgagcccc aaggcctcgg ggatttcaca
      241 cgtggtcgtc gtgatgatgg aaaatcgctc gttcgatcac ctcttcggat gggtacctgg
      301 tgcggacggc cgccaagctg gggcaagcta cgtcgatcgc gccggagtgc cgcatgcgac
      361 gcacgcgctc gccccagatt accaaggctg tggccatcca gatcccgacc actcgtacga
      421 aggagggcgc gtcgagtacg cggacggcgc gtgcgacggc tggctcaagg caggcagcaa
      481 cgacgacttt gcgattggtt actaccaggc gaacgacttg agtttcttct ctcgagccgt
      541 tccgcgcggg acggtgctcg atcgctactt cgccgccatc atggccgaga cgtacccgaa
      601 tcgaatctac cagcacgcgg ctcaagtgga ccggctgacg aacacgctcg acgtgagcac
      661 gttgccgacg atatgggaca ggctcgccgc agcgggacgg agcggacgct actattacag
      721 cgacgtcccg ttcttggctc tatggggccc caagtacctg ccgatcagtc gcccgctcgc
      781 agggttcgtg gtcgacgcgc tgctcggcac gttgccagac gttgccttcg tcgaccctcc
      841 atttctggac gagccgagcg gaacctccag cgatgaccac ccgcacgccg acatacgtcg
      901 cggcgaagcg ttcctgaacc tcctttattg ggccgtgacg cggggaagga gctgggacaa
      961 cacgctgctc gtcatcaact tcgacgagtg gggcggcttc ttcgaccacg tccctccgcc
     1021 aactgcacca atccccgcgg cggatcgcgc ggctggcaac gaggacgggc ggctcggctt
     1081 tcgcacgcca gcgctcgtga ttgggccctg ggctcgcgcc ggcgtggtgt cacacaccgt
     1141 gctcgatcac acctcgatac tgaagctcat cgagtggcgc tggggactcc cgccgctcag
     1201 cgagcgcgac gcttccgcga acaacctggc ggctgtactc gacttcagtc gcaggacgcc
     1261 gcggcctcca gcgttcgcgc cgccgctgca gccgttcggt acgccctgca cgtcgtcctt
     1321 gggcggtgac gaggtatcgt tgtcgtcact gcgcgagctg gcgcagacct tcggctggcc
     1381 catctatcag tgacgcgcgc gtcgggacgg tttcacccgg gaggtctcgg cgcttcgagc
     1441 gcgcggcgct agcgacagcg ctcgttggtt cagcgcgctt ccgtgaccgg cgtcacgacg
     1501 acgcggctat cgcgcttgtc gggacgacgg gtcgcttcga agaatcgcgg cgcggcggcg
     1561 gctgccactt cgaccgtcga actcgttcgc gctacgcgga ttgcgccgat gtccgagctc
     1621 ttgatctgcc ctcggcggca caacatcgcc accaaacgcc gcggatccgc gccgtgggct
     1681 tcaccccaag tgacgcggaa cggcacccaa ccgttgtcgt cgtcgcgcgc tcctcgcgcg
     1741 ttgcgctcag ggcgatccgc gcggggttca ctgcgcgggc ccgagcgcgg tctgaagtct
     1801 gcgcggtcac gcctttcgcc gccgagcgcg accggcgcga tctcacgtgg ctcaccgagc
     1861 gccttttgcg accgcgagag gagcagcgcg agcgcgctct cggcgagccc gctctcgatg
     1921 atgcgcgtca cttgcgctcg aatgcgcggg gagatttcgg tctgcgcgcg agaggcttcc
     1981 gtgagttcgg cgagccaacg cgcgtcctgg gcggcgcgga ttgcttcggc gttcggcact
     2041 tgcagcgtcc ggtaacgcac gcgagccctc tccaagatgc cgagcgtccg gcggagcgcg
     2101 tgcggtggga tgatgatcgc actcgtgccc ttgcgcccag cgcgccccgt gcgaccactg
     2161 cgatgggtgt aggtatccgg gtcggtgggg gcgtcgattt gaacgacgcg ggcgatgtct
     2221 tgcacgtcga tgccgcgcgc ggcgacatcg gtcgcgacca gcacgcgcac gtcgcgctgc
     2281 ttgaaggcgg cgagcgcgcg attacgagcg agctgctcca tctcgccgga gagcgcgccc
     2341 gcagcgaaac cggcgctttg gagctcacgc gtgatatcgg ccacctcggc gcgcgtgcgc
     2401 gcgaagacca acgtctggtc gttcgggtgc tccagcaaga cattgaccag tgcgtcgacc
     2461 tgggcgcccg ggcgcaccac gtagacgacg tgctcgatgt cgctgttcgc gctgccgagc
     2521 ggcgtgccct cgacgcgcgc tgggttcttc tgaatcgagt cggcgagcct cttcacctcg
     2581 ggagagaacg tcgcggagac gaggtgcgtg aagcgctcgc tgggcgtggt cgcgaggatc
     2641 gcctcgagct cgtcgcggaa cccgagatcc aacatgcggt cggcttcgtc gagcacgacg
     2701 gcgcgcaccg cgctcggatc gaccccgccg cgctgcaggt gatccagcaa gcgtcccggc
     2761 gttcccacca cgatggctgg gttcgacgcg agcgcgcggt gctcgtcacg gtagctggct
     2821 ccccccgtga ccgccgcgat cttcaagcgg aacggcgcga acaaccacga gagctcggcc
     2881 tcgacctgct tagcgagctc tcgagtcgga acgatcacca gcgcggccgg cttggcgagg
     2941 tgcgctttgc tggccggcgc ggcgtctcgc agcacgtcgc gcagcgcaaa gccgatggcg
     3001 agcgtcttac ccgaaccggt ttgcgagctc acgcggaggt cgcgatcgcg cagcgctgga
     3061 tcgaggaccg agagctgaac gggcgtgaga gtgagatagc ccttcttcgc gatcgccgcg
     3121 gagagctccg ggccgaggag ctcggacatc tcgaaatgaa ccacgggcgc gacggaggac
     3181 gacgattggg attgcatgcg gtgcgacggc gcgcgtgagg gtggcgcgcg gggcgccggt
     3241 tctagcagga cctgcggaat aagctcgtgg cgcgcgtatt tcggcctgaa aacgaacttt
     3301 gtctcggctc acgcaaggct tgcgtctggc aatggtcatc gaggacatcg gccgggttta
     3361 agcattcgta cgagccgacg tttcctgccg gcaccatgtc gatgctacgg tcctggtcca
     3421 tcggacaacg cctcatcgcg ctctcggcgt gtctgacgat cgcttctatc gttacactct
     3481 tctgcctcat caaggtcgtg gacaaggtgc gcgatctcgg cgtcgaagag gccggcgcgg
     3541 cggccttcga aggcgagaag accgcgttga aggtcggcgt cgacagcatg gcgctcacga
     3601 ttggcgcgac gatcgcgggc gtctccgacg agaagcaacg gctcgcgctc attaccaaga
     3661 tggtgacggc ggcgcgctac ggcagcgatc gcgcgggcta ctacttcgtg caccgaggta
     3721 cggtcgcggt ggtcatcccg cccgcgccgc aactgacggg gcaggatcgc gcgagcgaga
     3781 ccgacaagaa cggcgtcttc tacatcgtcg aactcgctcg taacgctggc accggcaagt
     3841 tcgtgaacta ccagttcgtg aagccgggcg gcaccgtggc gagcgacaag ctcggctact
     3901 cgcagctcat tccaggaacc gatctctgga ttggttcggg cgtgtacatc gacaacgtcg
     3961 cccgcgtgca gcacgggctc ggtgacaaga tcgtggcgct cacggacctg cgcatgcgac
     4021 ccgtgctcta tgtgctcggg gcgctctacc tgctcgtctt gccgtgcatc gtcctcgtca
     4081 atcgctccat cacgaagccg ctgagcgccg ccgtggatct cgcgaagcag gtggcagaag
     4141 gcgaccttcg catccacgtg gagcctcaac ccaaagacga gcccggtcag ctcatgagcg
     4201 ctctcgcttt gatgggcaag cgtctgcgtg acgtcgtcag tcaggtatcc gaagacgcgg
     4261 tttcgctcgc gagcagctcg agcgagctca cgggggcgtc gtcttcgctc gcgcaaggtt
     4321 ccaactcgca ggcgagctcg gtgagtcagg tgacggcggc gctcgagcag atcacggttt
     4381 cgatcgccaa ctccgccgac aaagctggac aaaccgagaa gcttgcggtg cacgccagcg
     4441 tgcaggtcaa cacgagcgcg gccaaggtcg tggagagcct cgatgcgatg cgctccgtcg
     4501 gtgacaagct gtccatcgtc gaggagattg cgcggcgcac ggacctgctc gctctgaacg
     4561 cctcgatcga ggcggcgcgt gccggtagtg ctggcaaggg cttcgccgtc gtcgccgccg
     4621 aggtgcgccg gctcgccgaa cgcagccgcg acgctgcttc cgaaatccgc gaactgacgc
     4681 agcgcacgat gctcctcgcg accgaagcgg ggcgctcgat gagccagatc gtccccgaga
     4741 tcgagcgcac ggcgagcctc gtgcaggaca tcgccgcgag ctcccgcgag atccgttcgg
     4801 gagccgcgag cgtcagcgac gcgatgcagc gcttggatgg cgtcgttcaa cacaacgccg
     4861 ccgcttccga ggagctgagc tccacgagtg agggcctctc ctcacgagcg gcggcgctgc
     4921 gcagcgcggt cggcttcttc accatcgacg acgcgaagtc ggtgctctcc atcgtcgaaa
     4981 gcgtccaagt ccggcgcgcc cctcgctcat tgccgagctt gactcgggca tgaggggcga
     5041 gccgaaagcg cgataaggcc gagctcgagc cgaagcgcga gcaggccgag ctcgactcgc
     5101 gctacttgat tccgaagctg aagcccgccg aagccttggc tttcgcgcca ctgttgccgg
     5161 tgctggcgtt ggcgctcgcg ctcttgttca cgttgacttt gggcggagtg acgttgactt
     5221 tcgcgttggc gccaacggct ttcgcagcgg cggagaggtt cgctttgacg tcgggaaccg
     5281 cgaccttcgc gccggctccg agtttggcat cggctccgag tttggcgtcg gccccgagct
     5341 tgccatcggc gcccagcttc gcgtcgggaa ggtttgcgcc gacgtcgagc tttgcgccgg
     5401 cggcagcctt cgcgtccagc cgtgcgttcg cgaccgcttg cagcgatgcg gccgcggctt
     5461 cgaatgcgcg cccgtccacg tccaagcgca gccggatcgg ttgcgctcgc aaatcgagct
     5521 ttccatcgaa ttcgccgcgg tagcttgcgc tcggagcgtc ccaggtgagc gcgacgttca
     5581 cgggcagagt ggcgccgagc gttagcttca aggccgcgtc ggctttggcc agggctttgc
     5641 ccgccgcgtc gaaggcgaat acgcgaacga cgttgcccgt tgggacgact tcgaacgagt
     5701 aatcgccggc gacgagcacc tcaccgccgt gcgcggcatc ggcgcgcaac gcgattcggt
     5761 ccaagccgcc aacggctaga cccgcggcgc ttcgaatcga aagctcgaag gcgcccggcg
     5821 cgagctcgac gcccgctcgt gcgtgaccgg cgaagcactt gcgagcatgg tcgaacgaaa
     5881 gcttcacgct ttcggcggcg cc
//
LOCUS       DASTKS010001302         6422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1550397, whole genome shotgun sequence.
ACCESSION   DASTKS010001302 DASTKS010000000
VERSION     DASTKS010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550397"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFQ35_27890"
     CDS             <1..910
                     /locus_tag="VFQ35_27890"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET9934570.1"
                     /translation="MLLPVVTDMKQAATALRWAVDEMERRYQLFANAGTKNIVTYNGW
                     VKKVLNGELPPPKPEIVQAVTHDGAVVDVPADKNGADGVSLPTKLPFIVVVVDEFADL
                     MMQQGKDVEASVARLAQKARAAGMHVVLATQRPSVDVITGMIKANFPTRIAFRVAQKV
                     DSRTILDEQGAEHLLGRGDMLVKLNGSTDTYRVQCPFVSEDEVSRLTDYLRAQGTPEY
                     NEAILRVPEDDTEDPSDQEPLDPRFDEAARIVAETQRCSTSWLQRKMTIGYNRAAKIV
                     EMMEKQGMVGPPNGARDRQVFLPPPS"
     gene            960..3731
                     /locus_tag="VFQ35_27895"
     CDS             960..3731
                     /locus_tag="VFQ35_27895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934571.1"
                     /translation="MIQRSAPAFPEALDDDDDDVTWALQTAGVQWKRGAHSDAIEWLH
                     RAVESAVDGGRAIRAREIQQQVNGLEAALKSGWRPDASSAAPPQPRAQRNPFLSESAD
                     LVHDPYGELAVDIDMESSFQLRSRLPTSALPQKAKAPFSSPAEDVSDDDLIEEVEEFD
                     EDDAAALAAPRMPFVSTPTLETDALEEDEFVEPDVEPEGELPTLPPSVRGLAPPGRRA
                     PSVPPRPPNLPATPSWPPPATNRPPTLPPSQRPDSVRPAASEPPPSEPPPSVRPLSER
                     PPSVPPASFPPQAAAWQQPSPPSSRPPSAPSRPRNRPSVPKISSSVPAAQAVVRSPSP
                     PSPPPPPTRNRPAPPRPQSVAASSREVRTTLGAGTPDSVSPRQPRSNRRPSARALALE
                     QKIAAAKALARQRRMEAGLPEEELPEVTAEIEPVPSSTSAPLRNASSHPPDTIVSSPP
                     ESFGSVPPEETTERDLEPQRGSASLPNIPRDSAPVLSYGELSDYDPNTSPNLETPFRR
                     RESLPPPSQAPESVPVESAPPPTTESEPSLPPSSRQVSESITAKHDLASEIAALMAAE
                     SEPEAPISEIPISEVPVSEVPQSAVPISVPLISEAPQSAAPISIPMISEAPHSAVPIS
                     VPLISAAPSAEALHSAAPISVPMLSAAPSPAAPEAAVPAPESAPPAGPRPTAISGIEL
                     GWVPGLEDLPEESQQDLVAAAEIHELGADEEVSSFELALVLEGSVSVMPAIADVAAAR
                     AGRGELIFSQGHLEPGLPIRVVASQAGAKVASFSKQSFEAAVRDCPWVGDELKGVGDR
                     LQTLAGVALGPLGEELDEMLRGLIVQHCEVRRFLPNEVIAPAGKAVPGMVIVGAGRIE
                     LVDTATNDVVDEVGSGSFLFASQILQAAPAPQTARAGEEGALALFAERRVAHELMVSV
                     PPLLGIFAR"
     gene            complement(3764..4399)
                     /locus_tag="VFQ35_27900"
     CDS             complement(3764..4399)
                     /locus_tag="VFQ35_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934572.1"
                     /translation="MLRFSPFWICLATTLVACTTKSVDLGEGPREYVANDYESVLRLW
                     TRSAELTNVSQMDNVLNVTATYESWDFRWAYVVRYAEDYRLTVEQRHDLLQRSLAESR
                     DMHTFYVALYSQKYKWNDLTAKQPAWIVRLIDDEGTETAPSQIDVIKRPGAIELTYFP
                     YTTPWRSVFRVSFPKVRADGRPTISSAARWFGIRFAGAQGNEELVWELAGT"
     gene            4581..6089
                     /locus_tag="VFQ35_27905"
     CDS             4581..6089
                     /locus_tag="VFQ35_27905"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET9934573.1"
                     /translation="MNRTLVGALALLAALTAAPRTTFARDLNAIRASGELRWGGDLQG
                     GEPYVFEDEAHPGKLMGFEVEIADALGRRLGVRPKFTQNDWSSLIPGLERDDFDIILN
                     GLEDTPERRARLGLSRPYFVYGETLTIRKSSGYASLDDLAGKRVGTLNQTVASDLLRQ
                     RPLEVVLYEGQEEPYFDLEKGRTEAVLLDHVIAERYGCPRPALRCLDGDVARGRYVIG
                     VARNNQALLRTLDDALAAMLREGELERILSHYKLWDARQKELTNELARPAPEPLPRAA
                     ESASSPPRRHLTASQLRLFVEGALMTLLLSFSSFALASPLGVLVGVLRVQGGRVARAI
                     CTVYVELFRGTPLLLQLYVLYFGISEVVRLGPITAAVLGLGLNYAAYEAEVHRGALQA
                     VPRGQTEAARSLGLSRWQTLRHVLLPQSLKNALPAVTNDFVALLKDSSLVSVITVVEL
                     TKRMTIVAVELRNWVTPGLLCAGLYFLMSFPLARFASRLERSLEHDQHSRLA"
     gene            6064..>6422
                     /locus_tag="VFQ35_27910"
     CDS             6064..>6422
                     /locus_tag="VFQ35_27910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9934574.1"
                     /translation="MISIRDLHKAYGERAVLGGISADVAAGQVVALVGGSGSGKTTLL
                     RCLNGLETFDSGRLEVAGHALSPGVNRRGLDALRADVGMLFQDYQLFPHLSVLDNVTL
                     APRIVRKIARREAERQG"
BASE COUNT         1086 a         2259 c         2044 g         1033 t
ORIGIN      
        1 catgctgttg ccggtcgtca ccgacatgaa gcaggcggcc acggcgctgc gctgggcggt
       61 cgacgagatg gagcggcgct accagctgtt cgccaacgcc ggcaccaaga acatcgtcac
      121 ctacaacggc tgggtgaaaa aagtcctgaa cggcgagctc cccccaccca agcccgagat
      181 agtgcaggcc gtcactcacg atggcgctgt cgtcgacgtg cccgcggaca agaacggcgc
      241 cgacggcgtt tcactgccga ccaagctgcc cttcatcgtc gtggtcgtgg acgagttcgc
      301 cgacttgatg atgcagcagg ggaaagacgt cgaagcctcc gtggcgcgac tcgctcaaaa
      361 ggcgcgcgcg gccgggatgc acgtcgttct cgccacccaa cgcccgagcg tcgacgtcat
      421 cacgggcatg atcaaggcga acttcccgac gcgtatcgcg ttccgcgtcg cgcagaaggt
      481 cgacagccgg accatcctcg acgagcaggg cgccgaacac ctgctcgggc gaggcgacat
      541 gctggtgaag ctgaacggct cgaccgacac ctaccgcgtg caatgtccgt tcgtgagcga
      601 agacgaggtc tcgcgcctga ccgactacct gcgcgcacag ggcacgcccg agtacaacga
      661 ggccattttg cgggtgccgg aagacgacac cgaggatcct tcggaccagg agccgctcga
      721 cccacgcttc gacgaagcgg cgcgaatcgt cgcggagacc cagcgctgct cgacttcatg
      781 gctgcagcgc aagatgacca tcggctacaa tcgggccgcc aagatagtag agatgatgga
      841 aaagcagggc atggtgggcc cgccgaacgg agcccgcgac cgccaggtct tccttccgcc
      901 cccctcgtga tgctgcacgc ctcccccctc cccccgctgt ggtatgaaag acggccggca
      961 tgatccagcg ctccgcgccc gcctttccgg aggcgctcga cgacgacgac gatgacgtaa
     1021 cgtgggcgct acaaacggcc ggtgtgcaat ggaaacgcgg tgcgcactcc gatgccatcg
     1081 agtggctgca ccgcgcggtc gagtcggcgg tcgacggggg ccgcgcaatt cgcgcccgtg
     1141 aaattcagca gcaggtgaat ggtctggaag ccgcgttgaa gagcggctgg cggcccgacg
     1201 cctcatcggc cgctccgccg cagccacgcg cgcaacgcaa ccctttcctt agcgagagcg
     1261 cggatctcgt gcacgatccg tacggcgagc tcgccgtcga tatcgacatg gagtcgtcgt
     1321 ttcagcttcg ctcgcggctc ccgacgagcg cgttgccgca aaaggcaaag gctccgttca
     1381 gcagccccgc cgaggacgta tccgacgacg acctcatcga agaggtcgaa gagttcgacg
     1441 aagacgacgc ggccgcgctc gctgcgccac gcatgccgtt cgtgagcacg cccacgctcg
     1501 aaaccgacgc gctcgaagaa gacgagttcg tcgagcccga cgtcgaaccc gaaggcgagc
     1561 tcccgacctt gccgccttcg gtccgtggtc tcgctcctcc ggggcgccgc gcaccttcgg
     1621 tgccaccacg cccgcccaac cttcccgcga cgccgagctg gccaccgcca gcaacgaacc
     1681 gccctcccac gttgccgccc tcgcagcgcc ccgattcggt gcgccctgcg gcatcggagc
     1741 cgccgccgtc agagccaccg ccttcagtgc gcccgctctc ggaacgtccg ccctcggttc
     1801 cgccggcttc gttcccgccg caagcagcgg catggcagca gccgagcccg ccctcgtcgc
     1861 ggccacccag cgctccgtca cgcccgcgca accgcccgag cgttccgaaa atcagctctt
     1921 ctgtgccggc cgcgcaggcc gtggtgcgct ccccctcgcc gcccagtccg ccgcccccgc
     1981 ctactcgcaa ccgtcccgcg ccgccgcgac cgcaaagcgt ggccgcgagt tcgcgagaag
     2041 ttcggaccac actcggagcc ggcacacccg acagcgtttc tccgcgacaa ccccgcagca
     2101 atcgccggcc ctccgcgcgc gcgctcgcgc tcgagcaaaa gatcgccgcg gccaaagcgc
     2161 ttgctcgaca gcggaggatg gaagccggtc tccccgaaga agagttgccc gaggtcaccg
     2221 cggagatcga accggtgccg tcgtcgacga gtgcgccgct ccgcaacgcg agctcgcacc
     2281 cgccagacac gatcgtcagc tcgcccccgg agtcgtttgg ctcggttccg ccggaagaga
     2341 cgaccgagcg cgacctcgag ccgcagcgcg ggtctgcgtc gttgcccaac atcccgcgcg
     2401 actcggcgcc cgtgctgagc tacggcgagc tctcggatta cgatccgaac acgtctccga
     2461 acctggagac accattccgg cggcgcgaga gcttgcctcc gccttcgcaa gctccggagt
     2521 cggtcccggt cgagagcgcc ccgcctccga cgaccgagtc ggagccgtca ctgccgccct
     2581 cgtcgcgcca agtgagcgag tcgatcacgg cgaagcacga tctcgcctcc gaaatcgccg
     2641 cgctcatggc tgccgagtcc gagccggaag ctccgatttc ggagatcccg atttcggagg
     2701 tcccggtttc ggaggtgccc cagtcggcgg ttccgatttc ggttccgctg atttcggagg
     2761 cacctcagtc ggcggccccg atttctatcc ccatgatctc ggaggcccct cactcggcgg
     2821 ttccgatttc ggttcccctc atctcggctg ctccgagcgc cgaggctctg cactcggcgg
     2881 ctccgatatc ggttccgatg ctctccgcgg ctccgagccc ggcagctccg gaggctgcgg
     2941 tgccagcgcc cgagtccgcg ccgcccgccg gtccgcgccc caccgccatc agcggcatcg
     3001 agctcggctg ggtccccggc ttggaagact tgcccgaaga gtcgcagcaa gatctcgtcg
     3061 cggccgcaga aatccacgaa ctcggcgccg acgaagaggt ctcgagcttc gagctggcgc
     3121 tcgtgctcga gggctccgtt tcggtgatgc cggcgatcgc agacgtggcg gccgcgcgcg
     3181 ccggccgcgg tgaattgatt ttcagtcagg gccacctcga gccaggactg ccgatccgcg
     3241 tcgtcgcgag ccaagccggc gccaaagtcg cgagcttctc caagcaatcg ttcgaagcgg
     3301 cggtgcgcga ttgtccttgg gtcggcgacg agctgaaggg cgtcggcgac cgtttgcaga
     3361 ccctcgccgg tgtcgcgctc gggccactcg gcgaggagct cgacgagatg ctgcgcggcc
     3421 tcatcgtgca gcactgcgag gtgcgccgct ttttgcccaa cgaagtcatc gcccccgctg
     3481 gcaaggccgt gcccggcatg gtgatcgtcg gtgcgggacg catcgagctc gtcgacacgg
     3541 cgacgaacga tgtcgtcgac gaggtcgggt ctggaagctt cttgttcgcg tcgcagattc
     3601 tgcaggccgc acctgcgccg cagacggcgc gcgccgggga agagggggcg ctcgcgctct
     3661 tcgccgagcg ccgcgtcgct cacgagctga tggtcagcgt accgccattg ctcggcatct
     3721 tcgcgcggtg agtcgcggcg gtaagcggcc gcttgtcgcg gcgttacgtc cccgcgagct
     3781 cccacacgag ctcttcgttg ccctgcgcgc cggcgaagcg aatgccaaac cagcgcgcgg
     3841 ctgacgagat cgtcggacga ccgtcggcac gaactttggg gaagcttacc cgaaacacgc
     3901 tgcgccacgg agtcgtgtac ggaaaatagg tgagctcgat cgcgccaggc cgcttgatca
     3961 cgtcaatctg ggaaggcgcg gtctcggtcc cctcgtcgtc gatcaagcgc acgatccagg
     4021 ctggctgctt cgccgtgaga tcgttccact tgtacttttg cgagtacaac gccacgtaga
     4081 acgtgtgcat gtcgcgcgac tcggcgagag agcgctgcag gaggtcatgc cgctgttcga
     4141 ccgtgaggcg gtagtcttcc gcatatcgaa cgacgtaggc ccagcggaaa tcccacgact
     4201 cgtaggtcgc ggtcacgttg agcacgttgt ccatttggga cacgttggtg agctctgccg
     4261 accgggtcca aaggcgcaag acggattcgt agtcgttcgc aacgtactcg cgcggcccct
     4321 cgccgagatc cactgatttg gttgtgcagg cgacgagggt cgtcgcgagg caaatccaaa
     4381 aggggctgaa ccgaagcatc gcctctcagc atagtcagtc gtgcccgctt cgtcccgcag
     4441 aaccacacca aaccgcgaag cgagcgatcg tcggcgcgaa cgccccacaa agaagcccaa
     4501 agcgcccgtc cgccgaagca aaagcctcgg cccgcgcggt atgacttacc cattcgctgg
     4561 ccctaggcta ataaggactc gtgaacagga ctctggtcgg ggctctggct ttgctagcag
     4621 cgctcacggc cgcgccgagg acgaccttcg cgcgagacct gaacgccatc cgagcgagcg
     4681 gcgagctgcg ttggggcggc gacctccaag gcggcgagcc atacgtattc gaggacgagg
     4741 ctcaccccgg taaactgatg gggttcgaag tcgaaattgc agatgcgctc gggcggcggc
     4801 tcggagtgcg accaaagttc acccaaaacg actggtcgag cctcatcccc gggctcgagc
     4861 gcgacgactt cgacatcatc ctgaacgggc tcgaagacac gcccgagcgt cgcgcgcgac
     4921 tgggtctctc tcggccgtat ttcgtctacg gcgagacgct gaccatccgc aagagctcgg
     4981 gctacgcgag cctcgacgac ctcgcaggca agcgcgtcgg aacgctgaac caaaccgtcg
     5041 caagcgatct gctccgtcag cgccccctcg aggtggtgct ctacgagggc caagaagagc
     5101 cgtacttcga cttggagaag ggccgcaccg aggccgtgct gctcgaccac gtcatcgccg
     5161 agcgctacgg ctgccctcgc cctgcgctcc gctgcttgga cggcgacgtc gcgcgcggcc
     5221 gctacgtcat cggagtggct cgcaacaacc aagcgctatt gcgcacgctc gatgacgcgc
     5281 tcgcggcgat gctgcgcgaa ggcgagctcg agcgcatcct gtctcactac aagctctggg
     5341 acgcacggca aaaagagctc acgaacgagc tcgcccgccc cgcgccggag ccgctgccac
     5401 gcgccgcaga gagtgcgtcg tcgccaccgc gccgacacct caccgcgtcg cagctccggc
     5461 tcttcgtcga gggcgcgctc atgacgttgc tcctgtcgtt ctcgtcgttc gcgctcgctt
     5521 caccgctcgg agtgctcgtc ggagttctcc gcgtgcaggg cgggcgcgtg gcgagggcga
     5581 tttgcaccgt ctacgtcgag ctgttccgtg gcacgccgct cttgctccag ctctacgtgc
     5641 tctacttcgg catcagcgaa gtcgtgcgtc tcggcccgat cacggctgcg gttctcgggc
     5701 tcggattgaa ctacgctgct tacgaagccg aggtgcatcg cggcgcgctc caagcggtcc
     5761 cgcgcgggca gaccgaagcg gcgcgttcac tcggcctctc acgctggcag acgctgcgcc
     5821 acgtgctcct cccccaatcg ctgaagaacg cgcttccggc ggtgacgaac gacttcgtgg
     5881 cgctgctgaa ggattcgtcg ctcgtgagcg tcatcacggt cgtggagctc accaagcgca
     5941 tgacgatcgt cgcggttgag cttcggaact gggtaacgcc gggccttttg tgcgccggcc
     6001 tctactttct catgagcttt ccgctggcgc gctttgcgtc acgtctcgaa cggagcctcg
     6061 agcatgatca gcattcgcga cttgcataag gcctacggag agagagccgt gctcggcggc
     6121 atcagcgccg acgtcgcggc cgggcaagtc gtcgcgctcg tcggcggttc gggatcgggc
     6181 aaaacgacgc tgctccgctg cttgaacggg ctcgagacgt tcgactcggg gcgcctcgag
     6241 gtcgcggggc acgcgctgtc gccaggcgtg aatcgccgcg gcctcgacgc gctccgcgcc
     6301 gacgtgggca tgctgttcca agactatcag ctcttccccc acctctccgt gctcgacaac
     6361 gtcacgctcg cgccgcgcat cgtgcgaaag atcgctcgcc gcgaagccga gcgccagggc
     6421 at
//
LOCUS       DASTKS010001303         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1772622, whole genome shotgun sequence.
ACCESSION   DASTKS010001303 DASTKS010000000
VERSION     DASTKS010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772622"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFQ35_27915"
     CDS             <1..1041
                     /locus_tag="VFQ35_27915"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9934575.1"
                     /translation="DPHASDYVHATLRYGDELLADVGLRIKGESSFRKLDKKSAFKIK
                     FDEFVPHQLFHGLHRLTLNNMIEDPSFIAERLAYDVYRAAKLPAPRCNSALVYVNGDF
                     YGVYANIESEDKTFLSRWFASNDGNLYEEGNHDFLTGADGYFDLETNEAANDRSGLLQ
                     LIAVTEAADATNFSETVGVSLNLEHFLRYVAAEAAVNQWDGYAYTMFYPNNFRIYEDP
                     KDGRFVFLPWGMDMSMKPFRDSGRAYIHAYEISRQGDRPNTKITAGILYRKCLDEARC
                     RAAYALEIQRIASLYDELGVPELAAAYYQQIREYVYADLRKEYTNERFELAYQSVLGT
                     ITGRAASLRADL"
     gene            complement(1114..1995)
                     /locus_tag="VFQ35_27920"
     CDS             complement(1114..1995)
                     /locus_tag="VFQ35_27920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9934576.1"
                     /translation="MRSIRISQVLRYVIRVDTDLLKTFVAVIETGGFTKASARRHLTQ
                     STVSMQMKRLAEELGQPLFTANGRGRVPTGAAEVLFGYAERILALQEEALAALNGTAR
                     EVVRVGATQDFAEARLPGALRAFSASHSEVAVAVRVGQSRELERAVLDGELDLAVVFE
                     TGERPRAPFSSERGAWLGSPSFLPPRAGEPWPLALFEAPCVFRDAAMRSLDAAGIPWR
                     VAYSTPSLSGLLAAVRAGLAVTLRLARHSQKGLKPVPAAAGLPAAPRFRLSLVKGDRP
                     SAGARALATVLRAEAKR"
     gene            2076..2465
                     /locus_tag="VFQ35_27925"
     CDS             2076..2465
                     /locus_tag="VFQ35_27925"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET9934577.1"
                     /translation="MPYIDVLVSEDPSPELAQRLAAGITERTVRVLGRVFEHTAVSVR
                     FVPRAHWFVGGQSLAARGKTSFWLDVKVTTGTTTEDQKARYLAEITELMRSELGELHE
                     VSYLRVDEVAGEAWGFNGISQAERRRR"
     gene            2508..>3323
                     /locus_tag="VFQ35_27930"
     CDS             2508..>3323
                     /locus_tag="VFQ35_27930"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9934578.1"
                     /translation="MHRAVTRTFLLGAFGCACSRTPAASESSATVVRPSSTPHVVAAE
                     GEGQVSKPLVGASSGAPVETAPPNVPEFKPAFSGQTRAPAVKTQSAFRVTELTTDLDN
                     PWAVAFLPEGRMLVTEKHEGRLSELAPDGKRLAVVGGVPKVDGRDQGGLLDVALAPDY
                     GSSSLVYLSYYEPRDGGNGLAVARARLVDGAKPRLDDLKVVFRMEPTLDSTKHAGGRL
                     IFAPDGMLFVTLGERSILKGRVQARDLASDLGKIVRIRPDGTIPEDNPFVHEKG"
BASE COUNT          629 a         1040 c         1084 g          570 t
ORIGIN      
        1 gatccgcatg ccagcgacta cgtgcacgcg acgctgcgct acggagacga gctcctcgcc
       61 gacgtcgggc ttcgcatcaa aggcgaatcg agcttccgaa agctcgacaa gaagagcgcc
      121 ttcaagatca agttcgacga gttcgttccg catcagctgt ttcacgggct gcatcggctc
      181 acgttgaaca acatgatcga ggacccatcg ttcatcgccg agcgcctcgc ttacgacgtg
      241 tatcgggccg ccaagttgcc ggcgccgcgc tgcaacagcg ctctcgttta cgtcaacggc
      301 gacttctacg gcgtctatgc caacatcgag tcggaagaca aaaccttcct gtctcgctgg
      361 ttcgcgagca acgacggcaa cctctacgaa gaggggaacc acgacttcct gaccggcgcc
      421 gatggctact tcgatctcga aacgaacgaa gccgcgaacg atcggagcgg cctgctccag
      481 ctgatcgcgg tcaccgaggc ggccgacgcg accaacttct ccgaaacggt tggtgtgagc
      541 ctgaacctcg agcattttct gcgttacgtc gcagcggaag cggccgtgaa ccagtgggac
      601 gggtatgcgt atacgatgtt ttacccgaac aatttccgca tctacgagga ccccaaagac
      661 ggtcgattcg tttttttgcc gtggggaatg gatatgtcca tgaagccgtt ccgagactcg
      721 gggcgagcct acatccatgc ctacgagatt tcgcggcagg gagaccgccc gaacaccaag
      781 atcactgccg gcattctcta ccgaaagtgc ctcgacgagg cgaggtgccg ggcggcgtat
      841 gcgctcgaga tccagcggat cgcgtccttg tacgatgagc tcggcgtgcc cgagctcgcc
      901 gccgcgtact accaacaaat ccgagagtac gtttacgcag atctccgcaa ggaatatacg
      961 aacgaacgct tcgagctagc gtatcagtcg gtgctcggca ccattacggg gcgcgcggcc
     1021 tctctccgcg ccgacctctg acgctttgct tcgagtccga agtttcgtgt ggcgggccag
     1081 cggccgttga gcagcccgcg tggcgcgagg ccgtcagcgc tttgcttcgg cgcgaagcac
     1141 ggtcgcgagg gcgcgagctc cggcgcttgg ccgatcgccc ttcaccaagg acaggcggaa
     1201 gcggggcgcg gcgggcaagc cggcggcggc cggcacgggc ttgagcccct tctgcgaatg
     1261 ccgagcgagc cgcagcgtga cggcgagccc agcccggacc gcggcgagta agccgctcaa
     1321 gctcggcgtc gagtaggcga cacgccaggg aatgcccgcc gcgtcgagcg accgcatcgc
     1381 agcgtcgcgg aacacgcaag gggcctcgaa gagcgcgagc ggccatggtt caccagctct
     1441 gggcggcaaa aacgacggag agccgagcca cgctccgcgt tcactcgaga atggggcgcg
     1501 gggacgctcg ccggtctcga agacgaccgc gaggtcgagc tctccgtcca gcacggcgcg
     1561 ctcgagctct cggctctgac cgactcgaac cgcgactgcc acttcggaat gcgacgcgct
     1621 gaaggcgcgc agcgcgcccg gcaggcgcgc ttcggcgaag tcttgggtcg caccgacgcg
     1681 gaccacctct cgcgcggtcc cgttgagggc agcgagcgct tcttcctgca acgcgaggat
     1741 gcgctcggcg tagccgaaaa gaacctcggc cgctccggtt ggtacgcgac cgcggccgtt
     1801 cgcggtgaag agcggctgcc cgagctcttc cgccaaccgc ttcatttgca tgctgaccgt
     1861 cgactgagtc aggtggcgcc gggcggaggc cttggtgaag ccccctgtct cgatgaccgc
     1921 gacgaacgtt ttcaaaaggt cggtgtccac ccggatgacg tagcgcaaca cttgtgaaat
     1981 tcgaatggat cgcatcaaaa actttcgatt ttcaaatgtg tgtgcgcgcc ccatcttcgg
     2041 agacacggcc caggcgccgt agaaagcagt gactcatgcc atacatcgac gtcctcgttt
     2101 cggaagaccc cagcccggag cttgcccagc gcttggccgc gggcatcacg gagcgcacgg
     2161 tgcgtgtgct cggccgtgtc ttcgagcaca cggccgtgtc ggttcgcttc gtgccccggg
     2221 cgcattggtt cgtgggtggg cagtcgctcg ctgcacgagg caagacgagc ttttggctcg
     2281 acgtgaaggt cacgaccggc accacgacgg aggaccaaaa ggcgcgctac ctcgccgaga
     2341 tcaccgagct catgcgctcc gagctcgggg aactacacga ggtgagttac ttgcgcgtcg
     2401 atgaagtcgc gggagaagcc tggggattca acggcatctc acaagcggag cggcgccgac
     2461 gttgagcaac cgcggcgctg gcgccattct tgctttgaag gaacctcatg catcgagctg
     2521 taaccaggac cttcttgctc ggggcgttcg gatgcgcttg tagccgcact ccggccgcga
     2581 gcgaatcgtc ggcaaccgtc gtcaggccga gctcgacgcc ccacgtggtg gccgcagagg
     2641 gcgaggggca ggttagcaag ccgctcgtgg gagcaagttc aggtgcgccc gtggagaccg
     2701 ctccgcccaa cgtgccggag ttcaaacctg ccttttcggg gcaaacgcgc gcgcccgcgg
     2761 tcaagacgca gagcgcgttt cgagtgacgg agctgacgac tgacctcgac aacccttggg
     2821 cggtcgcctt tttgcccgag gggcgcatgc tcgtgacaga gaagcacgag gggcgcctga
     2881 gtgaactggc tcccgacggg aaaaggttgg ctgtcgtggg tggtgtccca aaagtcgacg
     2941 ggcgcgatca aggaggcctt ctggatgttg ccctcgcccc agactacgga tcgagcagct
     3001 tagtttacct cagctattac gagccgcgcg acgggggtaa tggtctcgcc gtagcgcgcg
     3061 cgcggctcgt cgacggcgcg aagccacgcc tcgacgacct gaaagtggtg tttcggatgg
     3121 agccaacgct cgactcgacg aagcacgccg gcggtcgtct gatttttgct ccggatggga
     3181 tgctattcgt gacacttggg gagcgctcga tcctgaaggg acgggtgcag gcgcgcgact
     3241 tggctagcga tctcgggaag atcgtacgaa tccgtcccga cggaacgatc ccggaggaca
     3301 acccattcgt ccatgaaaag ggc
//
LOCUS       DASTKS010001304         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1550740, whole genome shotgun sequence.
ACCESSION   DASTKS010001304 DASTKS010000000
VERSION     DASTKS010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550740"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1418
                     /locus_tag="VFQ35_27935"
     CDS             186..1418
                     /locus_tag="VFQ35_27935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934579.1"
                     /translation="MAEPNDDADRQSTADEAAEPSAPEPTSAPEKGAAKAAAGDTQVI
                     KDRNRRIREEAAAKRRQKRATEERRVAPARNLDTSELVDDAMARTTHAVAQFLRRNFN
                     VVQYVVLAAVVGGIGYQIYSYRHARSVALAADELEKGVRAEHARVGDATEPTPDQYTG
                     LSDTRANYPTDEARLKAAEAEYKKVVTDGSATTSALASLALGGIYFDQGKYKDAQAAY
                     EKVKSSPLAKVDPDARARAIEGAGLSLEAAGQLDAALTAFRELENSDIPGFAPLGQYH
                     QARLLAQKGQRSEAKALLEKALKKLSEKSDDAKAPPIAPGGGYLEHQARELMSSIDPT
                     AAPKTTGAAFDPEELNRLQAELAGANGKIDPQKLQELLKKMGGGKAPGAPAAPVEAPG
                     TPAPEGPSETPNPAGSAP"
     gene            1415..2734
                     /locus_tag="VFQ35_27940"
     CDS             1415..2734
                     /locus_tag="VFQ35_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9934580.1"
                     /translation="MSRSAFITLGLLASTVVGCTALGAGANPERPLWVQRPSGNIEVV
                     YSTSAVVAARHSGEPYERGQPEIDARGRRVFVGSSDGGLYAFHAEDGSQIWRFETLGF
                     VQCRPLYDAETDTLYFGSNDGALYRVDAKNGKLRWRFMTNAEVSRKPVLAGGLLYVTN
                     ANDTLLALNPESGQLVWHQHRAPATGMEVAGYAGVAIFREKAYTGFSDGTVLAFDAKT
                     GVERWQPVDLSAEAEQTLGTVPEQLDVDTTPVPDQIEAGAVVYVANYAGGVFALDAET
                     GAQVWSNPGVVGVTELTLWSEPGRAPKAPGALGEALPTTDGEPQRVLIASSGTAGLVG
                     LDPETGKERWRRRLPRGGASAPVPMLGAIMISASQLGVFLVSPLGGELIDGIHIAGGV
                     SMAPAAFGSRAFVLTNGGRFLGMHLTGPFGTESKRSFSETQFFREPG"
BASE COUNT          560 a         1045 c          970 g          424 t
ORIGIN      
        1 ccttgccgac gagctgccgc cgcctccgcc caagccctgg accccagaag cgcccggcga
       61 atagcgccgt tggtcacaat ccgtggcaaa cgatgccctc aggtcacgcg ggcacgcagc
      121 gccggggcgc ccacgtgctc cggccgcctc gacgcatccc ccgacctatg tgatatacgc
      181 gccccgtggc tgaaccgaac gacgacgccg atcgccaaag caccgctgac gaagcggctg
      241 agcccagcgc acccgagccg accagcgctc ccgaaaaggg agcggccaag gcggccgccg
      301 gggacacgca ggtcatcaag gaccgcaatc ggcgcattcg cgaagaagcc gccgcaaagc
      361 gacggcagaa gcgcgcgacc gaagagcggc gcgtggctcc ggcgcgcaac ctcgatacga
      421 gcgagctcgt cgacgacgcc atggcgcgca cgacgcacgc ggtcgcgcag tttctgaggc
      481 gcaacttcaa cgtagtgcag tacgtcgtgc tcgcggcggt ggtcggcggc atcggctatc
      541 agatttacag ttatcgccat gcgcgcagcg tcgcgctcgc ggcggacgag ctcgagaagg
      601 gcgttcgcgc cgagcacgct cgagtcggcg acgccaccga gccgaccccg gatcagtaca
      661 cgggcctcag cgacacgcgc gccaattacc caaccgacga ggcgcggctc aaggcggccg
      721 aggcggagta caagaaggtc gtcaccgacg gctcggcaac gacctctgcg ctcgcttcgc
      781 tcgcgcttgg cggcatctac ttcgatcagg gcaagtacaa ggacgcgcag gcggcttacg
      841 agaaggtcaa gtcgagcccg ctcgccaaag tcgatccgga tgcgcgggcc cgcgcgatcg
      901 agggcgccgg gctcagcctc gaagccgctg ggcagctcga tgccgccctg accgcgttcc
      961 gcgagctcga aaactccgac attccaggtt tcgcgccgct cggccagtac caccaagcgc
     1021 gcctgctcgc ccaaaaaggg caacgcagcg aagccaaggc actgctcgaa aaggccctga
     1081 aaaagctttc ggaaaagagc gacgacgcca aggcgccgcc gatcgccccc ggcggcggtt
     1141 acctcgagca ccaagcgcgc gagctcatga gctcgatcga tccgacggcc gcgcccaaga
     1201 cgaccggcgc tgccttcgat cccgaggagc tgaaccgact tcaggccgag ctcgcgggcg
     1261 ccaatgggaa aatcgatccg caaaagcttc aggagctctt gaagaagatg ggcggcggca
     1321 aagcgccggg cgcgcccgca gcgccagtgg aagcacctgg cacgcccgcg cccgaaggcc
     1381 cgagcgaaac gccgaacccc gctggcagcg caccatgagc cggagcgcgt tcatcacgct
     1441 cggcctgctc gcgagcacgg tcgtcggctg caccgcgctc ggcgctggcg caaacccgga
     1501 gcgcccgctc tgggtgcagc gtccgagcgg gaacatcgag gtcgtgtatt cgacgtcggc
     1561 cgtcgtcgcg gctcgtcact cgggtgaacc ttacgagcgc gggcagcccg aaatcgacgc
     1621 ccgcgggcga cgcgtgttcg tgggctccag cgacgggggc ctctacgcgt ttcacgccga
     1681 agacggcagc cagatctggc gcttcgaaac gctcggtttc gtccaatgcc ggccgctcta
     1741 cgacgctgag accgacacgc tgtatttcgg ctcgaatgac ggcgcgcttt atcgagtcga
     1801 cgcgaagaac ggcaagctcc gctggcggtt catgacgaac gccgaagtgt cacgcaaacc
     1861 ggtgctcgcg ggcggcttgc tgtacgtcac gaacgcgaac gacacgctgc tcgcgctgaa
     1921 ccccgaatcc gggcagctcg tttggcacca gcatcgcgct ccagcgaccg gcatggaagt
     1981 agccggctac gcgggcgtcg ccatattccg cgaaaaggcg tacaccggct tcagcgacgg
     2041 aaccgtcctc gctttcgacg cgaaaaccgg tgtggagcgc tggcagccgg tcgacctctc
     2101 cgccgaggcc gagcaaaccc tcggtaccgt tccagagcag ctcgacgtcg atacgacgcc
     2161 ggtgcccgac cagatcgaag ccggcgccgt cgtctacgtc gcgaactacg cgggaggggt
     2221 cttcgccctc gacgccgaaa cgggcgcgca ggtttggagc aatccgggcg tcgtgggcgt
     2281 caccgagctc acgctatgga gtgagccagg ccgcgctccc aaagccccag gagccctcgg
     2341 cgaagctctt cctacgaccg acggcgaacc ccaacgggtg ctcattgcat cttccggcac
     2401 cgccggtctc gttggcctcg atcccgaaac cgggaaagag cgctggcgcc gacgcttgcc
     2461 gcgcggaggt gcgtcggccc cagtgcccat gctgggagcg atcatgatct cggcgagcca
     2521 gctgggagtc ttcttggttt ctccgctcgg cggagagctc atcgacggca tccacatcgc
     2581 aggcggcgtc tccatggcac cagccgcgtt tggcagtcgg gccttcgtgc tgacgaacgg
     2641 cggtcgcttc ttgggaatgc acctgaccgg cccattcggc accgaaagca agcgctcctt
     2701 cagcgaaacc cagttcttcc gcgaaccggg ctaatctttc gcgagcccgg ctgaagccaa
     2761 agccaggccc gggctgcgct aacagggggg acccccgata ccaatggtat cattccgcct
     2821 agctgcatcg gggtgcccga gacgtgcgga ccgattccac aaagctcagg cgccgcttct
     2881 tggagggcgg gccgccgttc gagcatgctc agcgtagccg ccgggaggtc cgccactgaa
     2941 ccgctgctcg acgtcgattc tcgcctctcc ttcttcgaag tagggcgaac gtgtttagc
//
LOCUS       DASTKS010001305         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_431974, whole genome shotgun sequence.
ACCESSION   DASTKS010001305 DASTKS010000000
VERSION     DASTKS010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431974"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFQ35_27945"
     CDS             <1..1051
                     /locus_tag="VFQ35_27945"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9934581.1"
                     /translation="EPAKTYIERAIEAGKGVVTANKLLIAKHGPRLIEAAIRQRVDLA
                     FEASVGGGIPIIRTLREALASDSVSSVHAILNGTCNYILTRMREAKLPFPAALAEAQK
                     LGYAEADPTLDVDGHDAAQKLAVASMLAFDAQVDNAQILVEGIREIDEIDFRFADRFG
                     FTLKHLGIGHDRGDKLELRVHPALIRKTSVLANVDGVLNGVFLMGRALGPCLLVGRGA
                     GDMPTAVSVVADIVDVARSRIEGEPGLLTRSIQLSARELLPQDRIATRYYLRFDVGDH
                     PGVVGRIASALGQEGVSIEQMVQEGQGQGQDHAVPVCLTTHVTSEGAVRRALRAIASQ
                     SFLKSPPRLIRIEDV"
     gene            1048..1905
                     /gene="murI"
                     /locus_tag="VFQ35_27950"
     CDS             1048..1905
                     /gene="murI"
                     /locus_tag="VFQ35_27950"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9934582.1"
                     /translation="MSTSPPDPAAPLGVFDSGLGGLTVVHALRQALPAESIIYLGDTA
                     RVPYGTRSAETVIRYARGCARLLTERGIKALIVACNTVSAVAIELLRAELDLPVIGVV
                     EPGARTALSAIEDIAATVPAARIGVLGTAGTVASGAYPRAVGQVSTRYEVVAQAAPLL
                     VPLVEEGWLDGDVPRLVIRRYLAPLIEASVAVIVLGCTHYPLLAPLIREEAQRLAGRP
                     IAVVDSAAATADAVREWIQQDRIRATPRPSSRLELLVTDRPQSFANVASRFLGEEVPP
                     IELVDLAPG"
     gene            1975..2868
                     /locus_tag="VFQ35_27955"
     CDS             1975..2868
                     /locus_tag="VFQ35_27955"
                     /inference="COORDINATES: protein motif:HMM:NF004957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9934583.1"
                     /translation="MAAVIPIDLKGRRAFVAGVADDGGFGFWIAKALIEAGASVCVGT
                     WPPALGIFETLMRRGKLDDSRKLENGSLLDFERVYAIDAAYDTMADVPESVATNKRYA
                     GRGDFTIEGVASSLLKDFGDKPLDIVVHSLANGPEVHLDLLETSRNGYLSALSVSAYS
                     MVSMVKRFAPLMRPGGSFLSLTYLASERVIPGYGGGMNSAKAALESDTRVLAYQAGRK
                     YGVRINTISAGPYASRAASVTGIINDVIEYYEKNAPIQERLEPREVASVAAFLSSPLA
                     SGITGTTVYVDKGYHAMGKGT"
     gene            2966..>3900
                     /locus_tag="VFQ35_27960"
     CDS             2966..>3900
                     /locus_tag="VFQ35_27960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934584.1"
                     /translation="MASPSDDYPALVRLTGPHHMDPSVENDQTRAERRLGHIVGRYRL
                     ERVLGVGGMAAVYVGRSQEGFVAAIKILHDDMSVRRDVRERFYREGYVANSVQHPGVV
                     RVLEHGETGPDAFLAMELLSGETLAERAKRVQALPVGEVLGIADQVLDTLRAAHEKGI
                     IHRDLKPDNLFITHAGQIKILDFGLARLAESAPGDQRTRSGVALGTLPYMAPEQALGR
                     RGEIDGRVDLFALGATLFRLLSGRRLHEATSEAELLIAMATRTAPPLISVAPHVPIDV
                     CRVVDLALAFARDARYPSAEVMQNDVRAVAAGQHPP"
BASE COUNT          625 a         1283 c         1306 g          686 t
ORIGIN      
        1 ggagcccgcg aagacttaca tcgagcgcgc gatcgaggcg ggcaagggcg tggtcactgc
       61 caacaagttg ctcatcgcga agcacggccc gaggctcatc gaggctgcga tccgccagcg
      121 cgtcgacctc gcgttcgaag cttcggtcgg cggcggtatc cccatcattc gcacgctgcg
      181 cgaggcgctc gccagcgact cggtctcgag cgtgcacgcc atcttgaacg gcacttgtaa
      241 ctacatcctc acgcgcatgc gcgaggcgaa gctcccattc ccggcggcgc tcgccgaggc
      301 gcagaagctc ggttacgcgg aggcggaccc gacgctcgac gtcgacggtc acgacgcggc
      361 ccagaagctc gccgtcgcga gcatgctcgc cttcgacgct caggtcgaca acgcccagat
      421 cttggtggag ggcatccgcg aaatcgacga aatcgatttc cgcttcgccg atcgattcgg
      481 cttcacgctg aagcatctcg gcatcggtca cgatcgcggc gacaagctgg agctccgggt
      541 tcaccccgcg ctcatccgca agaccagcgt gctcgcgaac gtcgacggcg ttttgaacgg
      601 cgtcttcttg atgggccgag ccctcgggcc gtgcttgctc gtcggtcgtg gcgcgggcga
      661 catgccgacg gcggtcagcg tcgtcgccga catcgtcgac gtggcgcgtt cgcgtatcga
      721 gggcgagcca gggctcctca cgcgcagcat tcagctctcc gcgcgcgagc tgctgccgca
      781 ggatcgcatc gcgactcgat actatttgcg cttcgacgtg ggcgatcacc ccggcgtcgt
      841 cggccgcatc gcttccgcgc tcggccaaga aggcgtgagc atcgagcaga tggtgcaaga
      901 agggcaaggg caggggcagg atcacgccgt gcccgtgtgc ctgacgacgc acgtcacgag
      961 cgaaggcgcc gtgcgccgtg cgctccgcgc gatcgcatcc caatcgttcc tgaagtcacc
     1021 cccacgcttg attcgcatcg aggacgtgtg agtacgtcgc cgcccgaccc ggcggcgccg
     1081 ctcggcgttt tcgactccgg cctcggcggg ctcaccgtgg tccacgctct gcgtcaggcg
     1141 ctgcccgccg agtcgatcat ctacttgggt gacacggcgc gcgtgccgta cggaacgcgc
     1201 tccgcagaga cggtcattcg ctatgcgcga ggctgcgcga ggctcctcac cgagcgtggc
     1261 atcaaagcgt tgatcgtcgc gtgcaacacg gtgagcgcgg ttgcgatcga gctcttgcgc
     1321 gccgagctcg acttgcccgt cattggcgtc gtcgagcccg gagcgcgcac tgcgctcagc
     1381 gcaatcgaag acatcgccgc gaccgttccg gccgcgcgca tcggcgtgct cggaacggcc
     1441 ggaaccgtgg cttcgggcgc gtatccgcgc gcggtggggc aggtgagcac ccgctatgag
     1501 gtggtggccc aagctgcgcc gctgctcgtt cctttggtcg aagaaggctg gctcgacggc
     1561 gacgtgccgc gactcgtgat tcgtcgctat ctggcgcctc tgatcgaggc ttcggtcgcc
     1621 gtcatcgtgc ttggctgtac acactatccg ctgctcgcgc cgctcattcg tgaagaggcg
     1681 cagcgcctcg ccggcaggcc catcgccgtg gtcgacagtg ccgccgcgac tgctgacgcg
     1741 gtgcgcgaat ggattcagca agatcgtatc cgggctacac cgcgaccctc ttcgcgattg
     1801 gagctcttgg tgacggatcg cccgcagtcg ttcgcgaacg ttgcgagccg tttcttggga
     1861 gaagaagtcc ctcccatcga gctcgtcgat ttggcgccgg gctgactcgg agctttcccg
     1921 gctgaccggt ccgcgcgccg tcgccctgtt gcaacgaagc aaaagatggg cgatatggcc
     1981 gccgtgattc ccatcgattt gaaaggtcgc cgcgctttcg ttgccggggt cgcggacgac
     2041 ggtggcttcg gtttctggat cgcgaaggcc ctcatcgagg cgggcgccag cgtgtgcgtc
     2101 ggcacgtggc ctccggcgct cggaattttc gagacattga tgcgtcgggg aaagctcgac
     2161 gactcgcgca agctcgagaa cggcagcctg ctcgacttcg agcgcgtgta cgcgatcgat
     2221 gcggcctacg acacgatggc ggacgttccg gagtcggttg ccaccaacaa gcgttacgcg
     2281 gggcgcggag attttacgat cgaaggcgtg gcgagttcgt tgctgaagga ctttggcgac
     2341 aagccgctcg acatcgtggt gcattcactt gcgaacggcc ccgaagtgca cctcgatctg
     2401 ctcgagacga gccgtaacgg ctacctctcg gcgctcagcg tcagcgccta ttcgatggtc
     2461 tcgatggtga agcgcttcgc tccgctcatg cgcccgggcg gctctttcct ttcgctcacg
     2521 tacctcgcga gcgagcgcgt gatccccggt tacggcggcg gcatgaacag cgccaaggcc
     2581 gcactcgaga gcgatacgcg cgtgctcgct tatcaagcgg gtcgcaaata cggtgttcgg
     2641 atcaacacga tctcggccgg gccgtacgcg tcgcgcgccg cgagcgttac cggcatcatc
     2701 aacgacgtca tcgagtacta cgagaagaac gcgcccattc aagagcggct cgagccccgc
     2761 gaggtagcga gcgtggccgc gttcctctcg agccctctcg cgagcggcat cacgggtacg
     2821 accgtgtatg tcgacaaggg ctaccacgcg atgggtaagg gcacctgagg cctccccgac
     2881 gggtggggtg tcccggcaaa ccggccaatt tcgccgaatc gtggccgctt ccggcaggtt
     2941 cgccccgctg ctcgcgtaag ggtttgtggc ttctccgtcg gacgactacc ctgcactggt
     3001 ccgtctgact gggccgcacc acatggatcc gagcgtcgaa aatgaccaga cgcgtgccga
     3061 gcgtcgactc ggtcacatcg tgggccgcta tcgcctcgag cgagtgctcg gggtcggggg
     3121 catggccgcc gtctacgtgg gacggagcca agaagggttc gtcgcggcga tcaagatcct
     3181 gcacgacgac atgagcgtgc ggcgcgacgt gcgcgagcgc ttttatcgcg agggatacgt
     3241 cgccaacagc gtgcagcatc ccggcgtcgt acgggtgctc gagcacggtg agactggtcc
     3301 ggacgcgttc ttggcgatgg agctcttgag cggagagact ctcgccgagc gcgccaagcg
     3361 cgtgcaggcc ttgcccgtcg gtgaggtgct cggcatcgcg gatcaagtgc tcgacaccct
     3421 gcgcgcagcg cacgaaaagg gcatcattca ccgcgatctc aagcccgaca atttgttcat
     3481 cacgcacgcg gggcaaatca agattctcga cttcggtctc gcccggctcg cggagagcgc
     3541 cccgggagac cagcgcactc gctccggggt ggcgctcggc acgctgccgt acatggctcc
     3601 cgagcaagcg ctgggcaggc gaggggagat cgacggccgc gtggatctgt tcgcgctcgg
     3661 cgcgacgttg tttcgcttgc tgtcagggcg gcgcttgcac gaggcgacga gcgaagcgga
     3721 gctcctgatc gcgatggcga cgcgcacggc tccgccgctc atcagcgtgg cccctcacgt
     3781 cccaatcgac gtgtgccgcg tcgtcgatct ggcgctcgcc ttcgcgcgcg acgcgcgcta
     3841 cccgagcgcg gaggtgatgc aaaacgacgt gcgcgcggtc gcggccggtc agcatccgcc
//
LOCUS       DASTKS010001306         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1998369, whole genome shotgun sequence.
ACCESSION   DASTKS010001306 DASTKS010000000
VERSION     DASTKS010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998369"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFQ35_27965"
     CDS             complement(<1..375)
                     /locus_tag="VFQ35_27965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934585.1"
                     /translation="MRRFASLTSLVLASMPALAHADLDFARARAAFQARHGASAALPR
                     ALANAPGDRVGLVLERQVPDAPPPPGATPLGGDFFGLTLPRAQASGLGEVPGFIVHWA
                     PERHVLLDRALAWCKVPEFRAAF"
     gene            complement(419..1168)
                     /locus_tag="VFQ35_27970"
     CDS             complement(419..1168)
                     /locus_tag="VFQ35_27970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934586.1"
                     /translation="MMGRVWKALALLSLSAACDGRFAFEVSAAGGSGGISLGSGGSSS
                     GGFVASGGTTPQGGSTGGAPTVGLGSSGGMVAAVVECLTRCALLDLACSVNSYPTLTC
                     IECLDDSGCRDFHDRPYCGPRNRCVGCLENRDCPERQLCVADTHSCAVRCDNDAACAG
                     LTPKPWCHVERGLCERCQSDDDCAGTPSTPHCDLMGVGCVACADDRHCSNGTVCDPVL
                     HRCVQCEASSDCPTGQICAPETHTCREIPVE"
     gene            complement(1165..2328)
                     /locus_tag="VFQ35_27975"
     CDS             complement(1165..2328)
                     /locus_tag="VFQ35_27975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET9934587.1"
                     /translation="MTEQDFSRLAASLLSRNAEDRLPRIVDREHGIAIVARAITERAR
                     KKRRARLLVYGAAFASAAGVALVAGFLGARHATSAADATLNCSALGTCAPAAPALQVG
                     LVDGRSFVPGQSLRARAGESTRVEFAHATEVALDPQTELEYRQGDTTRRFGLLRGAVH
                     LRVGKLAPGQRFVVETPDTEVEVRGTEFHVALAQPGAGCVSPRTSVSVDEGVVEVRFH
                     GETYRVTPGGHWPERCEIEAPPAAPAPPITQPSELRLEAPSAPARVPVHEKRVAAATK
                     AASPAAVEAGSQPPESDAQSDATSALTEQNNLYAKAVAARRAGRSLEALATYEQLLER
                     FPNGALCESARAERLRLLIRVNPAKAKVEAARYLARYPRGLASADARALLDQP"
     gene            complement(2325..3011)
                     /locus_tag="VFQ35_27980"
     CDS             complement(2325..3011)
                     /locus_tag="VFQ35_27980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9934588.1"
                     /translation="MSRDGRSRAFRLIRGGAVAAETAPEGPVSDRAALTDLQMVTAVR
                     EGNAAAAALFHNRLRPIVDRTLGRLIGAHDSDYDDLTQLALIQLVLSVDRFRGECPLD
                     AWASIIVARVAYKHIRKRRTERKLFVLDRDEIPDVIDRAASGAVAVRSAIRCVEKYLR
                     GMDENKAWTFVLHDVHGYDLEEVSAITGVSRAAAQSRLVRGRRALHERIAKDPELSSL
                     LTDLARKGDA"
     gene            complement(3149..>3875)
                     /locus_tag="VFQ35_27985"
     CDS             complement(3149..>3875)
                     /locus_tag="VFQ35_27985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934589.1"
                     /translation="SRRGQRWIDREVVFETTDPERERGRTLGFLLASMVLHAANDEEA
                     PPTVETIADGARSSLEVPPPDVPARPSEEADASASSLAATAELASAGAASGYGFWFGV
                     ERAVAARELWIGGGAHARFGSIVEAQASSRLLGLGVHMTWLPVRSGSLIFGVRLGGSL
                     AQLSAKRWAGSGTGEESQDRVLPSAEVLSLLGLRLAPRSVFTAQLGGEFLSGVTEVSV
                     RGQARATWPWAALLTRVGVETAF"
BASE COUNT          657 a         1371 c         1284 g          563 t
ORIGIN      
        1 gaacgccgcg cgaaactcgg gaaccttgca ccaggcgagc gcccgatcga gcagcacgtg
       61 ccgctctggc gcccaatgga cgatgaaccc gggaacctct ccgagcccgc tcgcttgtgc
      121 gcgcggcaac gtgagcccga agaagtcgcc tccgagcggc gtggcgccgg gtggaggcgg
      181 cgcgtcggga acctgtcgct cgagcacgag gccaacgcgg tcacctggcg cgttcgcgag
      241 cgctcgcggc aacgcggcgg acgctccgtg gcgcgcctga aaggctgcgc gcgcacgtgc
      301 gaagtcgaga tcggcgtggg cgagcgccgg catgctcgcg agcacgagcg atgtcagcga
      361 ggcgaagcgg cgcactcggt cagcttaccc caggggagga ccgagcccgc ggcagacgtc
      421 actctaccgg gatttcgcgg cacgtatgcg tttcgggcgc acaaatttgg cccgtggggc
      481 agtcggacga tgcctcgcac tgcacgcagc ggtgcaacac gggatcgcac acggtgccgt
      541 tcgagcagtg ccgatcgtcg gcgcacgcga cgcagccgac gcccatcagg tcacagtgcg
      601 gcgtcgatgg agttcccgcg caatcgtcgt cgctctgaca gcgctcgcac aagccgcgct
      661 cgacgtgaca ccagggcttc ggagtgagcc cggcgcacgc ggcgtcgttg tcgcaccgca
      721 ccgcgcacga atgcgtatcc gcgacgcaga gctgccgctc cggacaatcg cgattttcga
      781 gacagccgac gcagcgattg cgcggcccgc agtagggtcg atcatgaaag tcccggcagc
      841 ccgagtcatc gagacactcg atgcacgtca gcgtcgggta cgagttcacc gaacaggcga
      901 ggtcgaggag ggcgcagcgc gtgagacact cgaccaccgc cgcgaccatg ccgcccgagc
      961 ttcccaagcc cacggtcggc gcccccccgg tgctaccgcc ttgcggcgtc gtcccgccag
     1021 aagccacgaa gccgccgctc gaactgcctc cgctgcccag cgaaataccg cccgacccgc
     1081 ccgcggcgct gacctcgaaa gcaaaacgcc cgtcgcacgc ggcgctcaag ctgagcagcg
     1141 cgagcgcctt ccagactctg cccatcatgg ctgatcgagg agcgcgcgcg catcagcgct
     1201 cgcgagaccc ctcgggtagc gcgccagata acgcgccgct tccacttttg ccttggccgg
     1261 gttgacgcga atgagcagac gcagccgctc ggcacgcgcg ctctcgcaca gagcaccatt
     1321 cggaaagcgc tcgagcaact gctcgtaagt ggcgagcgcc tccaacgagc gacccgcgcg
     1381 acgcgccgca actgccttcg cgtacaggtt gttctgctcg gtcagagccg aggtagcatc
     1441 gctttgcgcg tcgctctccg gcggttgcga gccggcttcg accgctgccg gcgaggccgc
     1501 cttggtcgcc gccgcaacgc gcttttcgtg caccggcacg cgcgcgggcg cgctcggagc
     1561 ctcgagacgc agctccgaag gctgcgtaat tgggggcgcg ggagcagcgg gcggcgcctc
     1621 gatctcgcac cgctccggcc aatgcccgcc gggagtaacg cggtacgtct cgccgtggaa
     1681 gcgcacctcg accacgcctt cgtcgaccga aacgctggtg cgcggagaaa cacacccggc
     1741 gcctggctgc gccagagcga cgtggaactc cgtaccgcgc acctcgacct cggtgtccgg
     1801 tgtctccacg acgaagcgct gtcccggagc tagtttcccg acgcgcaagt gcaccgcccc
     1861 gcgcagcagc ccgaagcggc gcgtcgtgtc gccctgccgg tattcgagct ccgtttgcgg
     1921 atcgagcgcg acttcggtcg cgtgagcgaa ctcgactcgc gtgctttcgc cagcgcgcgc
     1981 tcgcaagctc tggccgggca cgaagctacg cccgtcgacg agtccgactt gcaacgcggg
     2041 cgcggccggc gcgcaggttc ccaatgccga gcagttcagc gtggcgtccg cggcggaagt
     2101 cgcgtgacgc gcacccaaga acccagcgac gagcgcgacg cccgccgccg aggcgaacgc
     2161 cgctccgtag acgagaagcc gcgcgcggcg ctttttccgc gcccgctccg tgattgcgcg
     2221 cgcgacgatg gcaatcccgt gctcacgatc gacgatacgc ggcagtcgat cttccgcgtt
     2281 gcgcgaaaga aggctcgcgg cgagccggga aaaatcttgc tcggtcacgc gtcccccttg
     2341 cgcgcgaggt cggtgagcag cgacgagagc tcaggatcct tcgcgatgcg ctcgtgcagc
     2401 gcgcggcgac ctcgcacgag ccggctttga gcggcggcgc gactcacacc ggtgatcgcg
     2461 ctgacctctt cgagatcgta gccgtgcacg tcgtggagga cgaaggtcca agccttgttt
     2521 tcgtccatgc cacgcagata cttctcgacg caccggatcg cgctacgcac cgcgacggcg
     2581 cccgacgcgg cgcggtcgat gacgtcgggg atctcgtcgc gatcgagcac gaagagcttg
     2641 cgctcggtgc gccgcttacg aatgtgcttg tacgcgacgc gcgcgacgat gatggaagcc
     2701 catgcatcga gcggacactc gccgcggaaa cggtccacgc tgagcaccag ttgaatcaac
     2761 gcgagctgcg tcaagtcgtc gtagtcggag tcgtgggcac cgatgaggcg tcctagcgtg
     2821 cggtcgacga tggggcgcag ccggttgtga aacaatgcgg ccgcggccgc gttgccctca
     2881 cgcacggcgg tgaccatctg caggtcggtc aacgcggcgc ggtcgctgac gggtccctcg
     2941 ggggctgtct ccgcagcgac tgcccctccg cgaatcaggc ggaaggctcg ggagcgacca
     3001 tctctcgaca tgctactggt cctaggtgcc cgcgagaggc tgcgagcagg cgccgaaaca
     3061 tcaacttacg agagacgcgg cgcgctcgcc agctcggccc cccgtgatct cgccgtcgaa
     3121 cgaccgaaat tctcctcgag gtttttcttc agaaggcggt ttccacgccc acgcgagtca
     3181 gcagcgcggc ccaaggccac gtcgcgcgcg cctgtccgcg gaccgaaacc tctgtaactc
     3241 ctgataaaaa ctcgcctcca agctgtgctg tgaagacgga gcgcggcgca agcctcaggc
     3301 ccaacaagct cagtacctcc gcgctcggaa ggacccggtc ctggctctct tcaccggtcc
     3361 ctgagccagc ccagcgcttc gcggagagct gcgccaagga gcctcccaag cggacgccga
     3421 agatgagcga ccccgagcgc acgggtagcc acgtcatgtg gacaccgagc cccaacaaac
     3481 gtgagctcgc ctgcgcttct acgatcgagc cgaagcgagc gtgcgctccc ccgccaatcc
     3541 agagctcgcg ggctgcgacg gcgcgttcga ccccgaacca aaacccatag ccgctcgctg
     3601 cgcctgcgct cgcaagttcc gccgttgcag caaggctcga ggcgcttgcg tccgcttctt
     3661 cggagggtcg ggcgggaaca tcgggagggg gcacctccaa cgagctgcgc gcgccgtcgg
     3721 cgatcgtttc gacggtgggc ggcgcctctt cgtcgttcgc cgcgtgcaac accatcgagg
     3781 cgagcagaaa gcccagcgtt cgtccacgct cgcgctccgg gtcggtcgtc tcgaacacga
     3841 cttcgcgatc gatccagcgc tggcctcgcc gcgag
//
LOCUS       DASTKS010001307         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1327758, whole genome shotgun sequence.
ACCESSION   DASTKS010001307 DASTKS010000000
VERSION     DASTKS010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327758"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFQ35_27990"
     CDS             <1..475
                     /locus_tag="VFQ35_27990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934590.1"
                     /translation="EAHDIGAAEIDTGAPALKWTAVFGKGHARLYATSAIPTGLYRFG
                     DANHSGVLSLNFGIVSRLTWLDSEGREGFLGLEVGVMAFGLSKDTGQNGAPLTQIGIV
                     TGLGIAVPVANRSSPTQASINLHGWLEQNVTGSNDGRFAFIFGPSISIGNVGANL"
     gene            complement(472..1299)
                     /locus_tag="VFQ35_27995"
     CDS             complement(472..1299)
                     /locus_tag="VFQ35_27995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9934591.1"
                     /translation="MSLKDFFRREASGMVPAWRKWEHALSFQGSHASNWIARLAEVRP
                     GMQVLDVASGIGDPSIHLAEQVGSAGRVVATDLVPEALEFVSRRAREQAFSWLSTTLA
                     EMESLPFESGTFDAVTCRLGLMFAEDPVAALVEMRRVLKPGGRAALLAWGTPHQPLFA
                     STLGVIAEVTASEAPVGRAFRFQQRGTLETALRDAGFREASEESRTLPWPFEGTGASM
                     WTMFRELAGPTFNEELGALGAHTRASLDARVSQRLEAFRRGSVIDPTAAFVGGFGVA"
     gene            complement(1404..2108)
                     /locus_tag="VFQ35_28000"
     CDS             complement(1404..2108)
                     /locus_tag="VFQ35_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HET9934592.1"
                     /translation="MVEAASQDSLTLAPTADPRVFRGEDGTRYTLPEGWSHLAPGDAG
                     LTRRVKAMGPSWTIVEEVNRKRFARGIWAPQENIAAARAALEAERSTPEYQKRRAAEL
                     ERRKKAQATYAAEFEHEVRAFLRFDPSFAAVEEKLAQRVAAHATPVGSGTVARTERIS
                     IDRRAEAAVIAWLRHQTSSYDTLAIPRVRGARRKARRDIAQMSRALLDLHRRENAHTP
                     AACPLCKTLLLDSSKP"
     gene            complement(2123..2704)
                     /locus_tag="VFQ35_28005"
     CDS             complement(2123..2704)
                     /locus_tag="VFQ35_28005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9934593.1"
                     /translation="MSYVVGGIRCLAQVSEREIQELGDVLVDCVEGGASVSFMLPFSR
                     DRAASFWRNVADAVHRGERALLVSEDERGISGTVQLNFALPENQPHRADVAKMLVHRR
                     ARRRGVGAALMRAAEMVAMERGRSLLVLDTVTDSDAARLYERLGWVRVGEIPGYALLP
                     GGGQVGTTVYYRELGAGADAPTASALSLSSGRK"
     gene            complement(2701..3297)
                     /locus_tag="VFQ35_28010"
     CDS             complement(2701..3297)
                     /locus_tag="VFQ35_28010"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9934594.1"
                     /translation="MADSATGVDGQVATRLRELRAARGLSLEALAQCSGVSRSMLSVI
                     ERGESSATAALLAKVTAALGVTLASLFEPGPSSASVSSPLARRVDQPRWRDPESGYQR
                     RSVSPPGRAIDAHIVEVEFPPGARVAFENGPRMARVHQQIWLLDGVMNVTVGDTRYRL
                     ERGDCLAMTLEGPTMFHNPTKRRARYAVIVSSPIREEQ"
BASE COUNT          599 a         1175 c         1179 g          596 t
ORIGIN      
        1 cgaagcccac gacattggcg cagccgagat cgacaccggc gcgccggcgc tgaagtggac
       61 ggcggtgttc ggtaaggggc acgcacgcct gtacgcgacg agcgcaatcc cgaccgggct
      121 ctatcgattc ggggacgcga atcacagcgg cgtgctctcg ttgaacttcg gcatcgtgtc
      181 gcgcttgacc tggctcgatt cggaagggcg cgaaggcttc ctcggtctcg aagtcggcgt
      241 gatggcgttc gggttgagca aagacacggg gcagaacggc gcgccgctga cgcaaatcgg
      301 gatcgtcacc gggctcggca tcgccgttcc ggttgcgaat cgttcgtcgc cgactcaagc
      361 gtcgatcaat ctgcacggct ggttggagca gaacgtcacc ggcagcaacg acggacgctt
      421 cgccttcatt ttcgggccca gcatctcgat cggcaacgtc ggagcgaacc tttaagcaac
      481 cccgaacccg ccgacgaacg ccgcggtcgg atcgatcacg gaccctcgtc ggaaagcctc
      541 gagtcgctgc gagacgcggg cgtcgaggga agctcgcgtg tgcgcgccga gcgcgcccag
      601 ctcctcgttg aaagtcgggc ccgcgagttc gcggaacatc gtccacatcg aagcgccggt
      661 tccttcgaac ggccagggta gggtgcggct ctcttccgag gcttctcgaa agcctgcgtc
      721 gcggagggcg gtttctagcg tgccgcgttg ctgaaatcga aaggcgcgcc cgaccggtgc
      781 ttcgcttgcg gtgacctccg cgatgacgcc gagcgtgctc gcgaacagtg gctgatgcgg
      841 cgtgccccat gcgagcagcg cagctctgcc tccgggtttg agcacgcgtc gcatttcgac
      901 gagggccgcg accgggtctt ccgcgaacat caacccgagg cggcacgtga ccgcgtcgaa
      961 ggtgcccgac tcgaatggca aagattccat ttccgcgaga gtcgtcgaga gccacgagaa
     1021 agcttgctcg cgcgcccggc gcgacacgaa ctcgagagct tctggcacga ggtcggttgc
     1081 gacgactcgc cccgcgctcc cgacctgctc tgccaggtga atcgacgggt ctccaatgcc
     1141 actcgcgaca tcgagcacct gcattcccgg tcgcacctcc gccaagcgcg caatccagtt
     1201 gctcgcgtga gagccttgga agctcagcgc gtgttcccat ttccgccagg ccggaaccat
     1261 cccggacgcc tcgcggcgga agaagtcttt tagggacatg cctcctacga cgcgcgacga
     1321 cgagtcccgt gacgcgtgag tgctcgtttt tcgactagcg gatcttggct cgcagcaggg
     1381 cggggaactt gggacacccc tagctagggc ttcgaagagt ccagcagcag agtcttgcag
     1441 aggggacagg cggcaggggt gtgggcgttt tctcggcggt gcaaatcgag cagggcgcgg
     1501 gacatttgag cgatatcgcg tcgcgccttg cggcgagcgc cgcgaacccg cgggatggcg
     1561 agggtgtcgt acgagctcgt ttggtggcgc agccaggcga tgacggcggc ctcggcgcgg
     1621 cgatcgatcg aaatgcgctc ggtgcgcgcg acggttccgc tgccgaccgg cgtcgcgtgc
     1681 gcggcgactc gctgtgcgag cttctcctcg acggcggcga agctcgggtc gaagcgcaga
     1741 aacgcgcgca cctcgtgctc gaattcggcg gcgtaggtcg cctgtgcttt cttgcggcgt
     1801 tccagctcgg cggcgcggcg cttttggtat tcaggggtcg agcgctcggc ttcgagggcg
     1861 gcccgcgcgg cggcgatgtt ctcctgaggc gcccagatgc cgcgagcgaa gcgcttgcga
     1921 ttgacctctt cgacgattgt ccaagacgga cccatggcct tgactcgacg cgtgagcccc
     1981 gcgtcacccg gagctaggtg actccaaccc tcgggtaggg tgtatcgcgt gccgtcttcg
     2041 ccgcgaaata cgcgaggatc ggcggttggg gcgagggtca acgagtcctg ggacgcagct
     2101 tcgaccattg cggccgcaca gcctacttac gacccgagct caatgaaagc gctgatgcgg
     2161 tcggagcgtc cgcgcccgcg ccgagctccc ggtaatagac ggtggttcca acctgccccc
     2221 cgccgggaag cagcgcgtaa cccgggatct ctccgacgcg aacccaccca aggcgctcgt
     2281 acaggcgcgc cgcgtcactg tccgtaaccg tgtcgagtac cagcaagctc ctcccgcgct
     2341 ccatcgcgac catttcggcc gctcgcatga gtgcagcgcc cactccgcgc cggcgcgcgc
     2401 gccgatgcac gagcattttg gcgacgtcgg cccggtgcgg ctggttctcc ggcagcgcga
     2461 aattgagctg cacggttccg ctgatgccgc gctcgtcctc gctcacgagc agcgcgcgct
     2521 ctccgcgatg gacggcgtcg gcgacgtttc gccaaaacga ggccgcgcgg tcgcgtgaga
     2581 acggcaacat gaagctgacc gacgcgcccc cctcgacgca gtcgacgagc acgtcgccga
     2641 gctcctgaat ttctcgctcg ctcacctggg ccaagcagcg gatgccgcct acgacgtagc
     2701 tcattgctcc tcccggatcg gagagctgac gatgacggcg tagcgcgcac gccgcttggt
     2761 cggattgtga aacatggtcg ggccctcgag cgtcatggcg agacaatcgc cgcgctcgag
     2821 tcgatatcgc gtatcgccca ccgtcacgtt catcacccca tccaataacc aaatctgttg
     2881 atggacgcgg gccatacgag gaccgttctc gaaggcgaca cgcgcgccag gaggaaactc
     2941 gacctcgacg atgtgcgcgt cgatggctct accgggtggc gacacgctgc gccgctggta
     3001 gcccgactcc ggatcgcgcc agcgcggttg atctactcgc cgcgccaagg gcgagctgac
     3061 ggaggcgcta ctcggccccg gctcgaacaa cgatgcgagc gtcactccca gcgcggccgt
     3121 caccttggcg agcaacgccg cggtagcgct gctctccccc cgctcgatga ccgagagcat
     3181 cgaacggctg acgccgctgc actgcgcgag cgcctcgagc gacaagccgc gcgccgcgcg
     3241 gagctcccga agccgcgtgg ctacctgtcc atcgaccccg gttgcgctgt ccgccatatt
     3301 ggatatgcct atccaattta ttggatagcg tcaactcggg cgctttgcgt acccgcacga
     3361 cgtgcctggt gacccggttt tctgctggca ctcgcggcga gtgctctcct agggggtgtc
     3421 ccaagttcct gcccgtcgcg agagccaaga aacgcgagcg agaagcgcgc ccgaggagtg
     3481 agcactggag gaaggctgaa gcctttcgag gagcggagcg acgaggccgt gcttcgcagc
     3541 cgcaggatc
//
LOCUS       DASTKS010001308         8088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_879262, whole genome shotgun sequence.
ACCESSION   DASTKS010001308 DASTKS010000000
VERSION     DASTKS010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879262"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFQ35_28015"
     CDS             complement(<1..353)
                     /locus_tag="VFQ35_28015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934595.1"
                     /translation="MLGRSKLVSGVARVGQLFAAASLLTYCSGGDRNFQEAQQGGHAG
                     ASKSGGGGSSARGGGSGTGGTAKGGGTSLASGGDESSGGSLPDTSGGTGTSGGTTASG
                     GVSPMEGGSGGTIAQG"
     gene            complement(738..1625)
                     /locus_tag="VFQ35_28020"
     CDS             complement(738..1625)
                     /locus_tag="VFQ35_28020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9934596.1"
                     /translation="MSESSAEPSKEDPRPDIVKLGEKRLSRTERWLIAFVRRTFEPGL
                     VDRVIRRCQRAVGSTWIHHCTKYLRHVYGIERLPKLDPKQSFLLVCNHRSFFDLYVVI
                     GELVRRGLPQRIVFPVRSNFFYTSLLGLFVNGVMSFFAMYPPIFRERKQAALNVASLD
                     ELTWLLRRGGVFAGLHPEGTRKRDDDPYTLLPAQRGVGRIIRDAAVPVIPVFINGLIN
                     DLPEQVKSNFARTGRKIVVVFGEPIDFGDLMSAPSTPKVHQAIADRALEVVAALGAEE
                     RLHRAELEAETAGLPLPSR"
     gene            complement(1622..2410)
                     /locus_tag="VFQ35_28025"
     CDS             complement(1622..2410)
                     /locus_tag="VFQ35_28025"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HET9934597.1"
                     /translation="MSNDPDSLQEPAPFTELVARARSGLRRDVEALLAGLDEATLLVP
                     LAQDVPEAPEGERIELNQQLSIVPHLLPDDEGQLFSALFTYSEALGPIVEALGWQTSG
                     EPLKICEFPARVALEMALEVVDGREVLGLVLDPGSESELCLTRNELASMLAGRALPLV
                     AYVANIPEDEQASTLVAEAGDPPPPELIAALESWLSQTPSVLTQKLERTFNPDRDLEP
                     HLTLTLTVDDAADRAELFRQVTERIEGTLPPPGYLDVLFVESKP"
     gene            2477..3445
                     /gene="pip"
                     /locus_tag="VFQ35_28030"
     CDS             2477..3445
                     /gene="pip"
                     /locus_tag="VFQ35_28030"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET9934598.1"
                     /translation="MTARRTFYPEIQPYRSGYLPVGSGHSIYFEESGNPNGKPVVFLH
                     GGPGGGTSPKQRRFFDPERYRIVLFDQRGCGKSRPHASLVDNTTWHLVQDIETLREHL
                     GIARWQVFGGSWGSTLALAYAEKHPNQVTELVLRGIFLVRRWELEWFYQSGTSLLFPD
                     AWEKYEALIPPAERDDFMSAYYRRLTSADPAVQKAAARVWSVWEASTSHLLPDEEYIR
                     SCAGDEFSLAFARIESHYFVNGGFLSRENQLIEDVGKIRHIPAVIVQGRYDVVCPMQS
                     AYELAKAWPEARFAIVPDAGHSAYEPGIVHELITATDAFASAERTK"
     gene            3445..7857
                     /locus_tag="VFQ35_28035"
     CDS             3445..7857
                     /locus_tag="VFQ35_28035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET9934599.1"
                     /translation="MQLRRAVRTLKKLYGKRERRSYLGRTRAHLELRDLSESEHELLE
                     ASLQQAFGKLPKVRWIEVYRELSRLVVTFEDDAYTLEELLRVLESVERSQGVASAPFS
                     DVHWEHPGDDETSERLTAGMAADALGAAMGFGLRFSPIPASRIAGTLASLVSIVQTSP
                     RLRRPIDERVGPNRADLGMSMGAAVAYGLAQRPSSAFVELVHKASQLSAVRARRRVWE
                     AREPTLFAERRPPQSFRPVEPRPRPLPRGPIEEYGDRAWVVSLGGFSVSLLATRSVQR
                     AVAALFGGLPKPAKHGRDAFTAHLESQLAARGILVVDRAALRRLDRIDCLVLEGGLVA
                     RDRFEVRSVLCEAEMDDRDARRIVTELFDAARPIARQDKHGYVLEPLGSAVSRPSVEL
                     SARASDLGSQGGLVLALSRAGRVLALAEVEWIAQTGIEELIAAAHDAQMRVVIASNDD
                     TVLQGFAADDTISDTEGMERGVRRLQREGRGVCVVATASNPGLAAADIAIGLHREGEA
                     PPWGAHLLCRYDLSDARFLIFASITARQVAKQSVNIALGAATLGALVSAGGVLPLTAQ
                     RVIGVVNAASLVSMGNGMRGGISLSRRALPPPRDRTPWHALEARGVMHKLRTSDAGLT
                     KRDAIERRRPSLPARTAVGELVEAVSDELFNPLAPLLAAGAGLSAAVGSMGDATMVGG
                     VVLLNALVGGVQRFRTERAIRELGKNATRRALVRRAGMLLEVDAAELSRGDIVLLSAG
                     DVVPADCRILEAEALELDSSSMTGESLPVRKGAEASFSADVADRSSMLYDGTVVAAGR
                     ARAVVVAVGDDTEARRGAVAQKSDASRGGVERRLRSLIDLTGPIALSAGVGLIGSGLL
                     RRRKLEELVGSGVSLAVASVPEGLPLLATAAQLAAARRLTQHGALVRNIRSIEALGRV
                     NVICLDKTGTLTEGRIELGLVSDGQAPLGIGELGPRERYVLAAALRASDLLNVAQDPT
                     DAALVKAAAEIQVQSDEGRPAWQKLREVPYEAGRGFAASIGAAGDVSLRVSLLSVKGA
                     PEVVLRAAEEWQRDGGRAPIDVATAAALHQHASDLGRRGYRVLAVAERVLGSDETASP
                     ESPSGLTFLGFVAFRDAVRKSAKRAVEDLARTSVRAVMVTGDHPSTARAVAEEVGLLE
                     GREVLTGADLAELSDQDLDRRIDDVGVFARVTPPQKVRVVRALQRRGRVVAMVGDGAN
                     DAPAIRLANVGIALGERATSAAQAAADIVLTDERIESLARSIWEGRAMWASVRDAVSI
                     LVGGNLGEIGFTLGAGLVDGRPPLNARQLLLVNLLTDVAPAMAIALRPPSQDSLRALA
                     LEGPERSLGQPLNRDIIARAGVTALGASAAWMVDRLTSGHVRAGTTGLVALVGTQLGQ
                     TLLSGRFSQPVVATSVASAVALAAIVQTPGVSHAFGCRPLGPLSWATAIGASAAATAA
                     TLYFPGFIDVAMKKLHLDKPMFVEDREALPADAS"
BASE COUNT         1237 a         2776 c         2722 g         1353 t
ORIGIN      
        1 ccctgagcga ttgtcccgcc gctgccgcct tccatcgggg agactccgcc cgacgccgtg
       61 gttccgccgc tcgtgccggt gccgccggaa gtgtcgggaa gggagccgcc cgaactctcg
      121 tcgccgccgc tcgccaggct cgtcccgccg cctttggcgg tgccgccggt cccgctgccg
      181 ccgccgcgcg cgctcgagcc accgccgccg ctcttactgg cgccagcgtg gccaccctgc
      241 tgggcttctt ggaagttgcg gtcgccgcca ctgcaatacg tgaggaggct tgcggctgcg
      301 aagagttgtc caacacgtgc caccccagaa acgagcttcg atcgaccgag cattgttcaa
      361 cgtcctccga gccagcaaag ccgtaggtgc gcctgcgctg ctgcgcgcgt cacgagcagg
      421 ccgctgacca ccgtcaccga ggcgctaatc ttgctcgacg agccgattcc gtcaactgcg
      481 tgatcctcga cacacccccc gagaaatcgt tccagcgtga gtgtgcgagc gatgttgtct
      541 ccgctccacg ctcatccacg ccacggctcg gggtttcgcc catcggtcgg attgttaatc
      601 gtattggact tgctcccaac gatccgagcg cgcatcggtc ctcctctcaa ctgcgcaaga
      661 aagtgacgaa aattggcgcg gatcctcaat ggcacggcgc gcgcagcgtg tatcgcgtcg
      721 agacgcggcg gtttcgttca gcggctcggc agcggcagcc cggccgtttc ggcttcgagc
      781 tcggcgcggt ggagtcgctc ctcggcgccg agcgcggcga cgacttcgag cgcgcgatcg
      841 gcgatcgcct gatgcacctt cggggtcgaa ggtgcgctca tcaagtcgcc gaaatcgatg
      901 ggctcgccga acacgacgac gatcttacgg ccggtgcggg cgaagttgct cttcacttgt
      961 tcgggcaagt cgttgatgag cccgttgatg aagactggaa tcacgggcac ggctgcgtcg
     1021 cgaatgatgc gtccgacgcc gcgttgcgcg ggcaacaacg tgtacggatc gtcgtcgcgc
     1081 ttgcgagtgc cttccgggtg cagccccgcg aagacgccgc cgcggcgcaa gagccacgtg
     1141 agctcgtcca agctcgcgac gttcagcgcc gcctgcttcc gttcgcggaa gatgggcggg
     1201 tacatcgcga agaagctcat gacgccgttg acgaacaacc cgagcaggct cgtgtaaaag
     1261 aaattcgagc gcacggggaa cacgatgcgc tgcggcagcc cgcgacggac caactcgccg
     1321 atgaccacgt agaggtcgaa gaagctgcgg tgattgcaga cgagcaagaa gctctgcttc
     1381 ggatcgagct tgggaagacg ctcgatgccg tagacgtggc ggaggtactt cgtgcaatgg
     1441 tggatccagg tcgagccgac cgcgcgttga caccgccgga tgactcgatc gaccaggccg
     1501 ggctcgaagg tgcggcgcac gaacgcgatc aaccagcgct cggtgcggga caggcgcttc
     1561 tcgccgagct tgacgatatc ggggcgcggg tcttccttgc tgggctcggc ggagctctcg
     1621 ctcatggctt cgattcgacg aagagtacgt cgagatagcc gggcggcggt agcgttcctt
     1681 cgatgcgttc ggtgacctgg cggaacagct cggcgcggtc ggcggcgtcg tccacggtga
     1741 gggtcagcgt gaggtgcggc tcgaggtcgc gatctgggtt gaaggtgcgt tcgagctttt
     1801 gagtgaggac gctcggagtc tgcgaaagcc agctctcgag cgctgcgatg agctcggggg
     1861 gcggtgggtc accggcttcg gcgacgagcg tgctcgcctg ctcgtcttcg gggatgttcg
     1921 cgacgtaggc aacgagcggc agcgcgcgcc ccgccaacat gctcgcgagc tcattgcggg
     1981 tgagacagag ctcgctttcg ctcccgggat cgagcacgag gccgagcacc tctcttccgt
     2041 cgacgacttc gagtgccatt tcgagcgcga cgcgcgccgg gaactcgcaa atcttcaacg
     2101 gttcgcccga ggtctgccag ccgagcgctt ccacgatcgg cccgagcgcc tccgagtacg
     2161 tgaacagcgc ggagaagagc tgaccttcgt catccggaag caggtgaggc acgatcgaga
     2221 gctgctggtt gagctcgatg cgctcgcctt cgggagcctc cggtacgtct tgcgcgagcg
     2281 gcaccaacaa agtcgcctcg tcgaggccgg cgagcagcgc ttcgacgtcg cgtcgcaggc
     2341 cgctacgagc cctggcgacg agctcggtga agggcgcggg ttcctgcaga gaatcggggt
     2401 cgttcgacac gcccgcggcc taacacgaag ggttactcga cgcacggcgc cgcctcttct
     2461 accctgttcg ccctcgatga cagcgcggcg gacgttctat ccagaaatcc aaccttatcg
     2521 cagcgggtac ctccccgtag gcagcggtca ctccatttac ttcgaagagt ccggaaaccc
     2581 gaacggcaag ccggtggtgt ttctgcatgg cggtcctggt ggaggaacga gccccaagca
     2641 acgccgcttc ttcgacccgg agcgctaccg catcgttttg ttcgatcagc gcgggtgcgg
     2701 gaagagccga ccgcacgctt cgctcgtcga caacacgacc tggcacctcg tgcaggacat
     2761 cgaaacgctg cgagagcacc tcggcatcgc gcgttggcag gtgttcggtg gatcgtgggg
     2821 cagcacgctc gcgctcgcct acgccgaaaa gcacccgaac caggtgaccg agctcgtgtt
     2881 gcgcgggatt ttcctggtgc ggcgttggga gctcgagtgg ttttatcaga gcgggactag
     2941 ccttttgttc ccggacgcct gggagaagta cgaggcgctc atcccgcccg cggagcgaga
     3001 cgacttcatg tccgcgtact atcggcgtct cacgagcgcg gatccggccg tgcaaaaagc
     3061 ggccgcgcga gtctggagcg tttgggaagc gagcacgagt cacttgctgc cggacgagga
     3121 gtacattcga agctgcgcgg gggacgagtt cagtctcgcc ttcgctcgca tcgagagcca
     3181 ctacttcgtg aacggtggct tcttgtcgcg tgaaaaccag ctgatcgaag acgtcggcaa
     3241 gattcgtcat atccccgccg tgatcgtgca aggtcgctac gacgtcgtgt gcccgatgca
     3301 gagcgcttac gagcttgcca aggcctggcc cgaggcgcgc ttcgcgatcg tgcccgacgc
     3361 gggacactcg gcgtacgagc ccggcatcgt gcacgagctc atcacggcaa cggacgcgtt
     3421 cgccagcgcg gagcgtacca agtaatgcaa ctcaggcgcg cggttcggac tctcaagaaa
     3481 ctttacggaa agcgcgaacg ccggagctac ctcggtcgca cgcgagctca cctcgagctt
     3541 cgcgatttgt ccgagagcga gcacgagcta ctcgaggctt cgctgcagca agccttcggg
     3601 aagctgccca aagtccgctg gatcgaggtg tatcgcgagc tttcacgcct cgtcgtcacc
     3661 ttcgaagacg acgcctacac gctcgaggag ctcttgcgcg tgctcgagag cgtcgaacgt
     3721 tcgcagggcg tcgcctcggc gccgttttcg gacgtgcact gggagcaccc cggcgacgac
     3781 gaaaccagcg agcgtctcac cgctggaatg gccgcggacg cgctcggcgc ggcgatgggt
     3841 ttcggcctac gcttctcgcc gatcccggcg tcgcgcatcg cgggaacgct cgcgtcccta
     3901 gtttccatcg tgcaaacgtc gccgcgcttg cgccgtccca tcgacgagcg tgtcggtccg
     3961 aaccgcgccg atctcggtat gagcatgggc gcagcggtag cctacggcct cgcgcagcgg
     4021 ccgagcagtg cgttcgtcga gctcgtgcac aaggcgtcgc agctctcggc ggtgcgtgcg
     4081 cgccggcgtg tttgggaggc gcgcgagccc acgctcttcg cggagcgacg cccgccccag
     4141 agttttcgtc ccgtcgagcc gaggccccgg ccgttgccgc gcggtccgat cgaagagtac
     4201 ggcgaccgcg cttgggtggt gtcgctcggc ggcttttcgg tcagccttct cgcgacgcgg
     4261 agcgtccagc gcgcagtggc ggcgctgttc ggtggcctgc ccaagccggc caaacacggc
     4321 cgcgacgcct ttacggcgca tctcgagtcg cagctcgccg cgcgcggcat cctcgtcgtc
     4381 gatcgcgcgg cgttgcgtcg cctcgatcgc atcgactgct tggtgctcga aggcgggctc
     4441 gtggcgcgcg accgcttcga ggtgcgcagc gtgctctgcg aagcggagat ggatgaccgc
     4501 gacgcgcgtc gcatcgtgac cgagcttttc gacgcggccc gcccgatcgc tcgtcaggac
     4561 aagcacggtt acgtgctcga gccgctcggc tcggcggtgt cgcggccttc ggtcgagctc
     4621 tccgcgcgtg cttcggatct cggctcccaa ggtggcctcg tgctcgccct gtcgcgcgcg
     4681 ggccgtgtgc tcgccctcgc cgaggtcgag tggatcgcgc agaccggcat cgaagagctc
     4741 atcgcggccg cgcacgacgc tcaaatgcgc gtcgtgatcg cgtccaacga cgacacggtg
     4801 ctgcaaggct tcgccgcgga cgacaccatc tcggacacgg agggtatgga gcgcggcgtg
     4861 cggcgcttgc agcgcgaagg tcgcggcgtt tgcgtcgtcg cgaccgcctc gaacccgggg
     4921 ctcgcggccg cggacatcgc catcggtctg caccgggaag gagaggcgcc gccctggggc
     4981 gcgcacctgc tctgccgcta cgacctgtcc gacgcccggt tcctcatctt cgccagcatc
     5041 acggcgcgtc aggtcgcgaa gcagagcgtc aacatcgcgc tcggggcggc caccctcggc
     5101 gcgctcgtct cggccggcgg cgtgctgccg ctcaccgcgc agcgcgtgat cggcgtcgtc
     5161 aacgccgcgt cactcgtctc gatgggcaac ggtatgcgcg gcggtatttc gctgtcgcgg
     5221 cgggccttgc cgcctccgcg cgatcgcacg ccgtggcacg ccctcgaggc tcgcggcgtg
     5281 atgcacaagc tgcgcacctc cgacgcaggg ctcaccaagc gcgacgcgat cgagcgacgc
     5341 cggccctcgc tgccagcgcg caccgccgtg ggcgagctcg tcgaagccgt gagcgacgag
     5401 ctcttcaatc cactcgcgcc gctcttggcg gctggggcag gtctgtccgc ggcggtgggg
     5461 tcgatgggcg acgccacgat ggtgggcggc gtcgtgctgc tgaacgcctt ggtcggtggc
     5521 gtgcaacgct tccgcaccga gcgagccatc cgcgaactcg gaaaaaacgc gacgcggcgc
     5581 gcgctggtcc gacgcgccgg gatgttgctc gaggtcgacg cggccgagct ttcgcgcggc
     5641 gacatcgtgc tgctttcggc aggcgacgtg gtccccgccg attgccgcat cctcgaagca
     5701 gaggcgctcg agctcgattc gtcgagcatg accggcgagt cgctgccggt gcgcaagggc
     5761 gcggaggcat cgttctccgc cgacgtcgcg gatcgcagct cgatgttgta cgacggcacc
     5821 gtcgtcgcgg cgggacgcgc gcgcgccgtg gtcgtcgcgg tgggtgacga cacggaggcg
     5881 cgtcgcggcg cggtcgcgca gaagagcgac gcgagccgcg gcggagtgga gcgtcgcttg
     5941 cgctcgctca tcgatctcac ggggcccatc gcgctgagcg cgggcgtcgg cttgatcggc
     6001 tccggtctcc tgcgtcggcg caagctcgaa gagctcgtgg gcagcggcgt gagcctcgcc
     6061 gtcgcgtccg tgcccgaagg tctaccgctg ctcgccaccg ccgcgcagct cgccgcagcg
     6121 cgacgcctca cccagcacgg cgcgctcgta cgcaacatcc gcagcatcga ggcgctcgga
     6181 cgggtgaacg tcatttgtct cgacaagacc ggcacgctca ccgaaggccg catcgagctc
     6241 gggctggtca gcgacggaca ggcgccgctc ggcatcggcg agctcggtcc gcgtgagcgc
     6301 tacgtgctcg ccgccgccct ccgcgcgagc gatctgttga acgtcgctca agatccgacc
     6361 gacgccgcgc tcgtgaaggc ggcagcggaa attcaggtgc agagcgacga ggggcggccc
     6421 gcatggcaaa agctccgcga ggtgccttac gaggcggggc gcggcttcgc ggcgagcatt
     6481 ggcgcagcag gcgacgtttc gctgcgtgtg tcgttgctca gcgtgaaagg cgcgcccgaa
     6541 gtcgtgctgc gcgcggcgga ggaatggcag cgtgacggtg ggcgagcgcc catcgacgtc
     6601 gcgaccgccg ccgcgctgca ccaacacgca tcggatctcg gaaggcgagg ttaccgcgtg
     6661 ctcgccgtcg cggagcgcgt gctcggctcc gacgaaaccg ccagcccgga gtcgccgagc
     6721 gggctcacct tcttgggctt cgtcgccttc cgcgacgcgg tccggaagag cgcgaagcgc
     6781 gccgtcgaag atctggcgcg cacctccgtg cgagcggtga tggtcaccgg cgatcatcca
     6841 agcactgcgc gcgccgtcgc cgaagaggtc ggcttgctgg aaggccgcga agtgctcacc
     6901 ggcgccgacc tcgccgagct cagcgatcaa gatctggatc gtcgcatcga cgacgtcggt
     6961 gtcttcgccc gcgtcacgcc gccgcaaaag gttcgggtcg tgcgtgcctt gcaacgtcgc
     7021 gggcgcgtcg tcgcgatggt cggagatggc gccaacgacg cgccggccat tcgcctcgca
     7081 aacgtcggca tcgcgctcgg cgagcgcgcc acctccgccg cccaagccgc cgccgacatc
     7141 gtcctcaccg acgagcgcat cgagtccctc gcgcggtcca tctgggaagg ccgcgccatg
     7201 tgggcctccg tgcgcgacgc cgtttcgatc ctggtcggcg gcaacttggg tgaaatcggc
     7261 ttcaccctcg gcgccggctt ggtcgacggc agacccccgc tcaacgcgcg ccagctccta
     7321 ctcgtcaact tactcaccga cgtcgccccg gcgatggcga tcgcgcttcg gcccccttcc
     7381 caagactcac tccgcgccct cgctctcgaa ggtcccgagc gctcgctcgg acagcctctg
     7441 aaccgcgaca tcatcgctcg cgctggcgtc accgcgctcg gcgcgagcgc tgcttggatg
     7501 gtcgatcgac tcacctccgg ccacgtccgc gccggcacga ccggactcgt cgcgctcgtc
     7561 ggcacccaac tcggtcaaac cctcttgtcc ggccggttca gccaacctgt cgtcgcgacc
     7621 agcgttgcct ccgccgtcgc gctcgctgcc atcgtccaaa cccccggcgt cagccacgca
     7681 tttggctgca gaccgctcgg ccccctcagc tgggcaaccg ccatcggagc ctccgctgcc
     7741 gcgaccgcag caaccctcta cttccctggt ttcatcgacg tcgccatgaa aaagctccac
     7801 ctcgacaaac ccatgttcgt cgaagaccgc gaagcccttc ccgccgacgc aagttaaccg
     7861 cgctcccctt gcgctgggca cctttccgcg tgcgccgcgc gccgtgcacc cctccgcgtg
     7921 cgccgtgcac ccgtccgctt ggccgtgcgc ggtgctccgt gcgttgtgct ccgtgcgctc
     7981 gggatgcttt gcgcggtggc cgtcgccacc atgcagcccg gccgcccatg cgcgtttcca
     8041 ctcacacgaa gaggccaccg cgggagtttg atcttgacgt ccaagcca
//
LOCUS       DASTKS010001309         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_211038, whole genome shotgun sequence.
ACCESSION   DASTKS010001309 DASTKS010000000
VERSION     DASTKS010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211038"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /gene="ffh"
                     /locus_tag="VFQ35_28040"
     CDS             complement(<1..1379)
                     /gene="ffh"
                     /locus_tag="VFQ35_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9934600.1"
                     /translation="MFDTLAKGFREAQNRLAGLTELNEKNIQAALKEVRQSLLEADVE
                     LGVVKTFLADVEQKAVGSTVQTRVKHGGETRKVSAGEQFVKLCYDELIRLMSHEGEPL
                     DLSRAVTSVMMVGLQGSGKTTTTAKLARLLAKDGKKPLLVAADMQRPAAVEQLQVLGQ
                     QIDVPVFNIAGATPLEICKAAQAEAQKQKRDVIIYDTAGRLAIDEPLMEELSAIKKAV
                     TPNNILLVVDSMIGQDAVKTAKSFNERLGITGVVLTKLDGDARGGAALSIKEVTGAPI
                     AFVGMGETTDKLEPFRPEGMASRVLGMGDVVSLIKGFEDVVDQKKAEEDAARMMSGQF
                     TLDDFLNQIRMIQKMGSLRDLVDKIPGMSGMLPPGAADQLDEGELPRIEAMIQSMTKA
                     ERADPNTLVREPSRVKRISKGSGQPEQGVSELVQKFLFMRQMMGGMGQDLGFLGNIPG
                     LKNLAMARN"
     gene            complement(1533..2441)
                     /locus_tag="VFQ35_28045"
     CDS             complement(1533..2441)
                     /locus_tag="VFQ35_28045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934601.1"
                     /translation="MTFARPIAATKQLVDVESVLDSMVSLAWNEIRHRARLVKRYTRV
                     PAIEGDESRLGQLFLNLIVNAAQAIDARALTDGAITLSTTLEGDQVVVEVADTGASID
                     ESDLPHIFEPLFTTKPAGKRSGSGLTLCRTIVASHGGDIRVSSDDGKGSTFRVSLPVA
                     KRVPAPSEPPTSGDLRAKRSRILVIDDEPLLGQTLTYAFAGRHDLVLATSGRDALRQL
                     AEDSQFDLVLCDLMMPDVSGPKVFEVVEQQHPNLVPRFAFMTGGAFTERAQEFLERYP
                     GRRIDKPFTIGEIEQLLADIASSQAD"
     gene            3082..>3516
                     /locus_tag="VFQ35_28050"
     CDS             3082..>3516
                     /locus_tag="VFQ35_28050"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HET9934602.1"
                     /translation="MTTSKQGSKSRKAKPGAPRGKARAKSEAPREASREASDSDQGAD
                     AVDSADGDGEGDADGEWGEGEPLDEPVGPDVEDGEVLEATGEVISDEPVENEPESRRF
                     GSSALTKVDALQTYMREVQRHALLSPDEEHSLAVQYAKTGDVN"
BASE COUNT          557 a         1149 c         1139 g          671 t
ORIGIN      
        1 atgttgcggg ccatcgccag gttcttgagg ccggggatgt tgcccaagaa gccgaggtcc
       61 tgccccatgc cgcccatcat ctgacgcatg aacaagaact tctgaacgag ctcgctcacg
      121 ccttgctctg gctggcccga gcccttcgaa atacgcttaa cgcgggaagg ctcgcgcacg
      181 agcgtgttcg gatccgcccg ctccgccttc gtcatcgatt gaatcatcgc ttcgatgcga
      241 ggcagctctc cctcgtcgag ctgatcggca gcgccgggcg gaagcatgcc gctcatcccc
      301 gggatcttgt cgacgaggtc ccgcagcgac cccatcttct gaatcattcg aatctgattc
      361 aagaagtcgt cgagcgtgaa ttggcccgac atcatgcggg ccgcatcttc ttcggccttc
      421 ttctggtcga cgacgtcttc gaagcccttg atgaggctga cgacgtcgcc catgccgagc
      481 acgcggctgg ccatgccctc ggggcggaac ggctctagct tgtcggtggt ctcgcccata
      541 ccgacgaagg cgatcggcgc gccggttacc tctttgatgc tgagcgcagc gccgccgcgc
      601 gcgtcgccgt ccagcttggt gagcacgacg ccggtgatgc cgagccgctc gttgaagctc
      661 ttcgccgtct tcaccgcgtc ctggccgatc atcgagtcga cgacgagcag gatgttgttc
      721 ggcgtcaccg ctttcttgat cgccgagagc tcctccatga gcggctcgtc gatcgcgagg
      781 cggccggcgg tgtcgtagat gatgacgtcg cgcttctgct tttgcgcttc ggcctgagcc
      841 gctttgcaga tctcgagcgg ggtcgcgccg gcgatgttga acaccggcac gtcgatttgc
      901 tgtccgagca cctgcagctg ctcgactgcc gcgggccgct gcatgtctgc cgcgacgagg
      961 agcggcttct tgccgtcctt cgcgagcaaa cgcgcgagct ttgcggtggt ggtcgtcttg
     1021 cccgagcctt gcaggcccac catcatcacg gaggtgacgg cgcggctcag gtcgagcggc
     1081 tcgccctcgt ggctcatcaa gcggatgagc tcgtcgtagc aaagcttgac gaactgctcg
     1141 ccggcgctga ccttgcgcgt ctcgccgccg tgcttgacgc gagtctgcac ggtcgagccg
     1201 accgcctttt gttcgacgtc cgccaagaac gttttcacca cgccgagctc gacgtccgct
     1261 tcgagcagcg actgccgaac ctccttcaag gcggcttgga tgttcttttc gttgagttca
     1321 gtcagaccag cgaggcggtt ttgcgcttcg cggaaaccct tggccaatgt gtcgaacacg
     1381 gggcgggtat tagcacaggt gccgggcttt ccgcgcgttg gccgcgcagt gatcgcgccc
     1441 cgccgcgagc ccaggcattt ttggcgctcc agaccctgcg cctgcctccc cgtgaccatg
     1501 cgatgggggc cggacccgtc gcgccgggga cctcagtccg cctggctgct cgcgatgtcg
     1561 gccaagagct gctcgatttc gccgatggtg aagggcttgt cgatgcgtcg ccccgggtag
     1621 cgctcgagga actcttgagc acgttccgtg aaggcgccgc cggtcatgaa tgcgaagcgc
     1681 ggcacgagat tcgggtgctg ctgctcgacc acctcgaaca ccttcggtcc ggagacgtcc
     1741 ggcatcatca agtcgcacag caccaagtcg aactgcgagt cttccgcgag ttggcggagc
     1801 gcatcgcggc cgctcgtcgc cagcacgaga tcgtgccggc cggcgaaagc gtaagtcagc
     1861 gtttgcccga gcagcggctc gtcgtcgatg acgaggatgc gcgaacgctt tgctcgcagg
     1921 tcgcccgagg ttggcggctc gctcggcgct gggacgcgct tggcgaccgg aagtgaaacc
     1981 ctgaacgtcg aacctttgcc gtcgtcgctg ctcacgcgga tgtctccccc atgcgaagcc
     2041 acgatggtgc ggcaaagcgt tagaccggag ccgcttcttt tgcccgccgg tttcgtcgtg
     2101 aagagtggct cgaatatgtg cggcaaatcc gactcgtcga tgctcgcgcc cgtgtcggcg
     2161 acctcgacca cgacctggtc gccttcgagc gtcgtgctga gcgtgatggc gccgtccgtg
     2221 agcgcccgag cgtcgatggc ctgcgcggcg ttgacgatga ggttcagaaa caactgaccg
     2281 agccgcgact cgtccccttc gatagctggt acgcgcgtgt accgcttaac cagtcgcgcg
     2341 cggtgccgaa tctcgttcca cgcgaggctt accatcgaat ccagcaccga ctcgacgtcg
     2401 acgagttgct tcgttgcggc gatcggacgc gcgaacgtca ttgaatccct cacgatgttg
     2461 cgcgcgcgct ctgcaccctc gcgcgcgttt catcagtcgc tccaccatac cgcgtccgag
     2521 cgctctcatg ccaccgcctt ctttggtagg tctcgcgcgc tcgaaggctg cgcacctcgg
     2581 tgtggggttg cgctcgaggc gtcgttctcg gcggttcccg ccgcggagct ggcatcagcc
     2641 ggtcggccaa gctcgccctc gttcgcgagc ctgtcgcttg catctgaggc taccgatgcg
     2701 ggctcccccg tcgtcctccc aatcgaagat gcgcgaaccc acggtcgttc ggaagtcact
     2761 ctgcagcgcc gagtcggtcg gttcgctctt cgcgtcgcga tgttccctcc gtctccgctg
     2821 cgaatcgctc attgaggggt cgaggctggc agccgtgggc ctcgacgggc ctcgccgcgt
     2881 gcctcgcttg acgggcgacg cggctggtcg cacccttcgc tccttgttcg cgcgaaaaga
     2941 ttccggtgaa tttggaagtt tcctgtggga aatgaatccg cgaccaagtt cgagactccc
     3001 agggccgccc cacgtgcggc aatcggccca aatccaagtt accgcagccg agcgtggacg
     3061 tcgccgcgcg ggtaaggccc gatgaccacg tcgaagcaag gcagtaagtc ccgcaaggca
     3121 aagcctgggg ccccacgcgg caaagccagg gccaagtcgg aggctccgcg cgaagcgagc
     3181 cgcgaggcga gcgattcgga ccagggcgct gacgcggtcg attctgcgga tggggatggg
     3241 gaaggggacg cggacggtga gtggggggaa ggagagcctc tcgatgagcc ggttgggccc
     3301 gacgtcgagg acggagaggt tttggaagcg acgggcgaag tcatctccga cgagcccgtc
     3361 gagaacgagc cggagagccg acgcttcggg tcgtcggcgc tcaccaaggt cgacgcccta
     3421 cagacgtaca tgcgcgaagt gcagcggcac gcgctcctct cgcccgatga ggagcactcg
     3481 ctcgccgtgc aatacgccaa gaccggtgat gtaaac
//
LOCUS       DASTKS010001310         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_656606, whole genome shotgun sequence.
ACCESSION   DASTKS010001310 DASTKS010000000
VERSION     DASTKS010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656606"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VFQ35_28055"
     CDS             complement(<1..1057)
                     /locus_tag="VFQ35_28055"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9934603.1"
                     /translation="MRETQNLRVVDTAVLQSPSQVLQEIPLSDRAAELVFQARRELED
                     VLHGRDRRRLVIVGPCSIHDPEAAIDYASRLRALRERVSTDLLVVMRVYFEKPRTTVG
                     WKGLINDPHLDGTFDIPRGIRVARALLRDIAEMGVPAATEMLDPIIPQYIADLVTWTA
                     IGARTTESQTHREMASGLSMPVGFKNGTDGGLGVAINAMIAASQPHSFLGIDADGRVG
                     VVRTSGNPHGHLVLRGGSRGPNFGEAEVGAAVKKLLDAKVNPRVLVDCSHDNSQKDPS
                     RQPDVLAAVGAQMRAGSEFLLGAMIESNIVAGRQELRTDKRALTYGQSITDACVDFAT
                     TERMLEELARDASHRRAV"
     gene            complement(1254..>2464)
                     /gene="lnt"
                     /locus_tag="VFQ35_28060"
     CDS             complement(1254..>2464)
                     /gene="lnt"
                     /locus_tag="VFQ35_28060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET9934604.1"
                     /translation="RGERRGWWSGPLFLMAFVASELCFPVLFFWSYGGVAHPAPALTQ
                     VAELGGVYLVGLVMVAFNWAIAEVLLARLRGGKPELRRLLPYALAPVVAALYGAVRIR
                     QVDARALAAPSGEVGLVQANMGLMEKRQAYEDGLQRHIDHTAELRAKQPLDLVIWSET
                     SVMHAMPAEDAPRILPRYFTRRLHVPTLFGAVLFDRVSDARERAFYNSVLLADEKGEV
                     RGRYDKQELVPFSEHMPFGEEFPVLFEISPNSSNFRRGATAAPIAFGEHRVAAMVCND
                     DVSPSLGNRLLRDSRTDLLVNLTNDAWFGDTTEPWIHLALAKFRAIEHRRYFVRSTNS
                     GVSAFIDPVGRVIAKTATFSEETLRHRIHWLTGRTVYEWTGDALYWAAALFVFVAAFL
                     PKARLRKGAS"
BASE COUNT          465 a          803 c          775 g          421 t
ORIGIN      
        1 cgacggcccg ccgatgactt gcatcgcgcg cgagctcttc gagcatgcgc tcggtggtag
       61 cgaaatcgac acacgcgtcg gtgatgctct ggccgtacgt cagcgcacgc ttgtcggtac
      121 gcagttcctg acggccggcc acgatgttgc tttcgatcat cgcgcccagc aaaaattcgg
      181 acccagcgcg catctgtgcg ccgacggcag cgagcacatc gggttggcgc gacggatcct
      241 tctgactgtt gtcatggctg cagtcgacca gcacgcgcgg attcaccttg gcatcgagga
      301 gcttcttcac cgccgctccg acttctgcct caccgaagtt cgggccgcgg ctaccaccgc
      361 gcaggacgag gtggccatga gggttgccgc tggtgcgcac cacaccgacg cggccgtcag
      421 cgtcgatacc taggaagctg tgcggctgag aggctgcgat catcgcgttg atcgcgacgc
      481 cgagaccgcc gtcggtccca ttcttgaagc cgaccggcat cgagagcccg ctcgccatct
      541 cgcggtgggt ttggctctcc gtcgtgcgcg cgccgatcgc cgtccacgtc acgaggtccg
      601 cgatgtactg cgggatgatg gggtcgagca tctcggtcgc tgcaggcacg cccatttcgg
      661 caatgtcgcg caggagcgcg cgcgcgacgc gaatgccgcg ggggatgtcg aacgtaccgt
      721 ccaggtgcgg atcgttgatg agccccttcc aaccaacggt ggttcgcggc ttctcgaagt
      781 aaacgcgcat cacgaccaag agatcggtcg aaactcgttc acgcagcgcg cgcagtcggc
      841 tcgcgtagtc gatcgcggct tccggatcgt gaatcgagca cgggccgacg atgacgagcc
      901 ggcgccgatc gcgaccgtgc agcacgtcct cgagctcgcg ccgagcttga aagacgagct
      961 ccgccgcccg atcgctcagg gggatctcct gaagaacttg gctggggctc tgcagcacgg
     1021 cggtatcgac gactcggaga ttctgtgttt cgcgcatgac ggggatccgg aaagcaaaaa
     1081 gcccccggtg cctttcagcg accaggggct cgattcgttc gaccttttta cttcgttaca
     1141 ggtctgccct agccgccaca aggggggcgc cagtagccaa accaaaagta aaaacggccg
     1201 gacgacatga acgaacgaga tctagcgttt cttccttgaa agcgcaagct tcttcaggaa
     1261 gcaccctttc gaagacgcgc tttcggcaag aaggccgcga cgaagacgaa gagcgccgcc
     1321 gcccaataca acgcatcacc tgtccactcg tacactgttc ttccggtgag ccaatggatg
     1381 cgatgacgca gcgtttcttc gctgaacgtc gcggtcttcg cgatgactcg accgaccgga
     1441 tcgatgaagg cgctgacgcc gctgttcgtc gagcgcacga aatagcggcg gtgttcgatg
     1501 gcgcggaact tcgcgagcgc caagtggatc cacggctcgg tcgtgtcgcc gaaccaggcg
     1561 tcgttcgtca ggttcacgag gagatccgtg cgcgaatcgc gcagcaggcg attaccgagc
     1621 gacggactca cgtcgtcgtt gcagaccatg gccgcgacgc gatgctcgcc gaaagcgatg
     1681 ggcgccgcgg tcgcgccacg ccgaaagttg ctcgagttcg gcgaaatctc gaacagcacg
     1741 gggaactctt caccgaacgg catgtgctcg ctgaaaggga cgagctcctg cttgtcgtag
     1801 cgaccccgca cctcgccctt ctcgtcggcg agcagcaccg agttgtagaa ggcccgctcc
     1861 cgggcgtcgc tcactcgatc gaagagcacg gccccaaaca gcgtgggtac gtgtagtcga
     1921 cgcgtgaagt agcgcggcaa gatccgcggc gcatcctccg cgggcatggc gtgcatcacg
     1981 gaggtctcac tccaaatgac gaggtcgagc ggctgcttcg cgcggagctc cgcggtgtgg
     2041 tcgatgtggc gctgcaaccc gtcttcgtac gcttgtcgct tctccatcaa acccatgttc
     2101 gcttgcacca agccgacctc tccgctcggc gccgctagcg ctcgggcgtc gacttgacga
     2161 atgcgaaccg cgccgtagag cgcggccacg accggagcga gggcgtacgg tagcaacctg
     2221 cggagctcgg gtttgccgcc gcgaagccgc gcgagcagca cttctgcgat cgcccagttg
     2281 aacgcgacca tcacgagccc gaccagatag acacctccga gctccgcgac ttgcgtcaac
     2341 gccggcgctg gatgcgcgac gccgccgtag gaccagaaaa acagaaccgg aaaacagagc
     2401 tcgctcgcca cgaacgccat caagaagagg gggccgctcc accaccctct tcgctcgccg
     2461 cgca
//
LOCUS       DASTKS010001311         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_211604, whole genome shotgun sequence.
ACCESSION   DASTKS010001311 DASTKS010000000
VERSION     DASTKS010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211604"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFQ35_28065"
     CDS             <1..448
                     /locus_tag="VFQ35_28065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934605.1"
                     /translation="EVQRKRARLVQLEADWKRFGAERERAQKEVDRVERDVSLALAKD
                     REDLARYALKQALTQKNLVERIGARMAESAEEQRELGLALQSQQVTLEELRARVQAFL
                     SQRESGCLEVRVLPVTDEQIELELMRRKAQPAARAEDAPEARDGSK"
     gene            435..962
                     /locus_tag="VFQ35_28070"
     CDS             435..962
                     /locus_tag="VFQ35_28070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934606.1"
                     /translation="MARNSLLRGLAFALVAGTTAPFLVFAAGLPVASVALCTVVVHLL
                     VVAPNLRRGLHAAALASALALPCALFVSDQLTGIALGAVILGLCRSALLYPRSFARAL
                     VAELAFLGVGSVSALIWAGTAFVGVPLMTWSFWLVQAGFVLFQPDSRGSGDVPADPFE
                     RAQRLAEAVLAGRPH"
     gene            complement(988..2181)
                     /locus_tag="VFQ35_28075"
     CDS             complement(988..2181)
                     /locus_tag="VFQ35_28075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934607.1"
                     /translation="MRGVPKVLVTLLLVAACGSEKSPFAKGNGASGGRSGQGGAVGSG
                     GFTQLPSTRGGTTGSGGRVPDEPPGENVLTLLHGIVDAPAISICFAKGQTLDAPPLGM
                     PQPPGGLSYGSSLVLRSIDGIDWAKDTLSTWIIAGDLSLVEGLDCEQAVTLAKSEMAM
                     ASEAPEPSAEGGAGGAGGAAGAPGAGGEGGGFTAEPPRLRAARLPFVPAGTLSVGRSS
                     LMVAEGCIGGPAYQSRLGENACGAGYSKTSATLSAVLVQMSRRSMPFRLGLQAVHASQ
                     ANGAVGVTSAPIDGGASFVVVANSVELGGIAPRTLELNYDVSSWGVNTPDWQLEVSQN
                     STVLFAEPWSTVLARAGVSLLANGSAYALVFVGPGVGVPLGRNQLWNPPRIVLVPTDP
                     TPEEP"
     gene            complement(2192..3004)
                     /gene="thiD"
                     /locus_tag="VFQ35_28080"
     CDS             complement(2192..3004)
                     /gene="thiD"
                     /locus_tag="VFQ35_28080"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9934608.1"
                     /translation="MLPRVLIVAGSDSGGGAGIQADIKTVTMLGGFAATAITALTAQN
                     TLGVFGVSPVDPSFVREQMRVVLEDIGADAVKTGMLFSADIVRVVASELESRATSIPI
                     VVDPVMVAKGGAALLDERASGALVERLFPLAALITPNVPEAVALTGLEVNDEDDLPRV
                     AERLLTMGPSAVLVKGGHLPGERVVDVLRTIDGVEHRFEGPRIATRSSHGTGCTLASA
                     IATGVAEGLTLVSAIERARRYVVRALRTAPGLGQGHGPLNHGWLLAEKNRMN"
     gene            complement(3060..3842)
                     /locus_tag="VFQ35_28085"
     CDS             complement(3060..3842)
                     /locus_tag="VFQ35_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9934609.1"
                     /translation="MTARKNTARAIADLGAGTILATVDIAAPPERIFRALTTEEVTKW
                     WGSDEMYRTTEFAAELRVGGAWHAKGQGASGPPFIVQGEYLELDPPRKIVQTWRPDWD
                     QGATSTLTYLLEPTAHGARVTVRHEGFGDRRESCENHSNGWELVLGWLESHLSPASPA
                     AAKYFLCRLLPPRPSFAFDMSEAERRAMGEHVAYWTQKLEEGVAIVFGPVADASGPWG
                     LGVVRVPDEAALKAMTENDPAIRSGLGLSYQTLPMLNAVHRQ"
     gene            complement(3839..4159)
                     /locus_tag="VFQ35_28090"
     CDS             complement(3839..4159)
                     /locus_tag="VFQ35_28090"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9934610.1"
                     /translation="MHRARADTDVFRAIADPTRRAILDRLRAGPAHVNSLAADFEQSR
                     PAISKHLRVLRDSNLVSEQRFGRARVYQLEAGGLQRVAGWLEGYRSFWQSSLNNLKRY
                     LEDT"
BASE COUNT          668 a         1443 c         1492 g          734 t
ORIGIN      
        1 ggaggtgcag cgcaagcggg cgcgactggt gcaactcgaa gccgactgga agcgcttcgg
       61 cgccgaacgt gagcgcgcgc agaaggaggt cgaccgcgtg gagcgcgatg tgtcgctcgc
      121 gctcgccaaa gatcgcgaag acttggcgcg atacgcgctg aagcaagcgc tcacgcaaaa
      181 gaacctcgtc gagcgcatcg gcgcgcgcat ggccgagtcc gcggaagagc aacgggagct
      241 cggcttggct ctccagtcgc agcaagtgac gctcgaggag cttcgtgcgc gcgtgcaggc
      301 gttcctgtcg caacgtgaga gcgggtgcct cgaggtgagg gtgttgccag tgaccgacga
      361 gcagatcgag ctcgagctca tgcgtcgcaa ggcacagcca gcggctcggg ccgaggatgc
      421 accggaggct cgcgatggct cgaaatagcc tcttgcgtgg tctcgcgttc gcgctcgtgg
      481 ccggcacgac cgcgccgttc ttggtttttg cggcggggtt gccggtggct tcggtggctc
      541 tctgcacggt ggtggttcac ctcttggtgg tcgctccgaa tttgcggcga ggtctgcacg
      601 cggccgcgct cgcgagcgct ctggctctgc cgtgcgcgct cttcgtgtcg gaccagctca
      661 ccgggattgc gctcggggcc gtgattctcg ggctttgtcg gagcgcgctg ctctatccgc
      721 gttcgttcgc gcgagcgctc gtcgcggagc tcgcgttcct cggtgtcggc agcgtgtcgg
      781 cgctcatttg ggccggcacg gcgttcgttg gcgttccctt gatgacctgg tcgttttggc
      841 tggtgcaggc cgggttcgtc ttgtttcagc cggattcgag ggggtccggg gacgtgccgg
      901 cggatccgtt cgagcgcgca cagcgcttgg cggaggccgt tctcgccgga cgcccgcact
      961 gatcgatcgc gtccgcgacg cgcgcgctca cggctcttcg ggggttgggt ccgtcggcac
     1021 gagcacgatg cgcggcgggt tccagagctg gttgcgtccg agggggacgc caacgccggg
     1081 acccacgaag acgagcgcgt aggcgctccc gttggcgagc agggacaccc ccgcgcgggc
     1141 gagcacggta ctccagggct ccgcgaagag tacggtcgag ttctgcgaga cttcgagctg
     1201 ccagtcgggg gtgttcacgc cccatgagct cacgtcgtag ttcagctcca gtgtgcgcgg
     1261 cgcgatgccg ccgagctcga cgctattcgc gaccacgacg aacgatgcac caccgtcgat
     1321 tggcgcggag gtgacgccca ctgcgccgtt tgcttgcgaa gcgtgcaccg cctgcaaccc
     1381 caagcgaaac ggcatcgagc gccgtgacat ctgcacgagc actgcgctca gcgtggcgct
     1441 cgtcttcgaa tacccggcgc cgcaggcgtt ttcgcccagc cgactctgat acgcgggccc
     1501 gccgatgcat ccctccgcga ccatcaggga gctgcgcccg accgaaagcg ttccggccgg
     1561 tacgaagggt aggcgcgccg cgcggagccg aggaggctct gccgtgaagc cgccgccctc
     1621 accgcctgca ccgggtgcgc ctgccgcgcc tccggctccg ccggctcctc cttcggcgct
     1681 cggctcgggg gcttcggacg ccatcgccat ctcgcttttg gcgagcgtga cggcttgctc
     1741 acagtcgagc ccttcgacga gggaaagatc tccggcgatg atccaagtcg agagcgtgtc
     1801 cttggcccaa tcgatgccgt cgatgctgcg caacacgagc gacgagccgt agctgagccc
     1861 gccgggcggt tgtggcattc cgagtggcgg agcgtcgagc gtctgcccct ttgcgaagca
     1921 aatcgaaatc gccggtgcgt cgacgatgcc gtggagcaag gtgagcacgt tctcgcccgg
     1981 gggctcgtcg ggaacgcgcc cgccgctgcc agtggtgccg ccgcgcgtgg agggcagttg
     2041 agtgaacccc ccgctgccga ccgccccgcc ttgccccgag cgcccgcccg aggcgccgtt
     2101 gccttttgca aacgggctct tttcgctgcc gcaggcggca acgagcaaca gcgtcacgag
     2161 gactttcgga acaccgcgca aggcaccctc ctcaattcat ccgattcttt tcggcgagca
     2221 gccagccgtg gttcagcggg ccgtgaccct gcccgagccc cggagccgtg cgcagcgccc
     2281 gcaccacgta gcgtcgcgcg cgctcgatgg cgctcaccaa agtcaggcct tcggcgactc
     2341 cggtcgcgat cgccgaggcg agcgtgcagc ccgtaccgtg gctggagcgc gttgcgatgc
     2401 gtgggccttc gaagcgatgc tcgacgccgt cgatcgtgcg cagcacgtcg acgacgcgct
     2461 cacccggcaa atgtccgcct tttaccaaga cggcggaggg acccatggtc agcaggcgct
     2521 cggccacgcg cggaaggtcg tcttcgtcgt tcacctcgag gccagtcagc gcgaccgcct
     2581 cgggcacgtt gggcgtgatg agcgctgcga gcgggaagag ccgttcgacc agtgcgcctg
     2641 acgcgcgctc atcgagcaga gccgcgcctc ccttcgcgac catcaccggg tcgaccacga
     2701 tcggaatgga ggtggcgcgc gactcgagct cggacgcgac gacgcgcacg atgtcggcgg
     2761 agaagagcat cccggtcttc acggcgtctg caccgatgtc ctcgagcacg acgcgcatct
     2821 gctcgcgcac gaagctcgga tcgaccggcg agacgccgaa gactcccagg gtattttgcg
     2881 cggtgagggc ggtgatggcg gtcgccgcga agccgccgag catcgtcacg gtcttgatgt
     2941 ctgcttgaat gccggcgccg cctccggagt cggagcctgc cacgatgaga acacggggaa
     3001 gcaccggcga agcatcatcc gaagccgcgc ttcgcgcaaa tcggcccgag cgcaaccctt
     3061 cattgtcggt ggacagcgtt cagcatgggc agggtttggt agctcaaacc gaggcccgag
     3121 cggatcgccg gatcgttctc ggtcattgcc ttcaacgccg cttcgtccgg aactcggacg
     3181 accccgagcc cccaagggcc gctcgcgtcc gcgacagggc cgaagacgat cgcgactccc
     3241 tcctcgagct tttgggtcca gtacgccacg tgctctccca tggcgcgccg ctccgcctcg
     3301 ctcatgtcga aggcaaagct cgggcgcggg ggcaatagcc ggcacaggaa gtacttggcg
     3361 gcggcggggc tcgcgggaga aaggtgactc tcgagccaac cgagcacgag ctcccaccca
     3421 ttcgagtgat tttcgcacga ctcacggcga tcgccgaagc cctcgtgccg tacggtcacg
     3481 cgcgcgccgt gcgcggtcgg ttcgaggaga tacgtgagcg tcgacgtggc gccctgatcc
     3541 caatcggggc gccacgtctg aacgatcttc cgcggcggat cgagctccag gtattcgccc
     3601 tgtacgatga agggagggcc gctcgctccc tgtcccttcg cgtgccatgc gccgcccacg
     3661 cgtagctccg ccgcgaactc ggtggtgcga tacatctcgt ccgagcccca ccacttggtc
     3721 acctcttccg tggtcagcgc cctgaagatg cgctccggag gggcggcgat gtcgacggtc
     3781 gcaaggatcg ttccggcgcc taggtcggcg atggcgcggg cggtgttttt tcgggcggtc
     3841 atgtatcctc caggtaacgc ttcaggttgt tgagactgct ctgccaaaaa gaccggtagc
     3901 cctcgagcca gcccgcgacg cgctgtaggc cccccgcttc gagctggtag acgcgcgcgc
     3961 ggccaaagcg ctgctcgctc accaaattgg agtcgcgcag cacacgaagg tgcttggaaa
     4021 tggccggtcg actctgctcg aagtccgccg cgagcgaatt gacgtgggcg gggccggccc
     4081 gaagccggtc caggatcgcc cggcgggttg ggtcggcgat agctcgaaaa acgtccgtgt
     4141 cggcgcgagc gcggtgcatt gtgtaaccga ttggttactg atagccgtcc gcgcggctcg
     4201 agtcaaacaa ctttgtgcga tcgaatccgc cttcggctgc catctgtccg ctgtgcacgg
     4261 agaggcgcga ccgctcatca cgctcgtgaa agacccgccg gaccgagtcg agctcccgcg
     4321 ggggcgaatc gactccg
//
LOCUS       DASTKS010001312         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_2000480, whole genome shotgun sequence.
ACCESSION   DASTKS010001312 DASTKS010000000
VERSION     DASTKS010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000480"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /gene="serC"
                     /locus_tag="VFQ35_28095"
     CDS             <1..628
                     /gene="serC"
                     /locus_tag="VFQ35_28095"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET9934611.1"
                     /translation="NTIFGSQWHEIPNTGSVPLVADMSSDIMWRPMDVSKFGLIYAGA
                     QKNLGPSGLVVVIVRKDLVASGRQDIPVIFQYRTHSENNSLYNTPPTFSVYVLRNVLD
                     EVKKAGGLAAMEKINREKAKLLYDAIDERSDVFSAPVEKDSRSVMNVVFTLKTPEAEA
                     EFLAEAQKRGMIGLKGHRSVGGMRASIYNAVPVEAVKALADLVRSFKK"
     gene            complement(687..1412)
                     /locus_tag="VFQ35_28100"
     CDS             complement(687..1412)
                     /locus_tag="VFQ35_28100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934612.1"
                     /translation="MLHPRMRCAKSVVLLLTLVAGMAQAEPNASTPAEPHEEKLDAPT
                     PTSEPPAPANAAPPSAPTPPVAPPAETRVAPSHPPSSSAGRAQVRFAVSRPEAWLEAR
                     PFGSGDPWQPRCKAPCGEWVEVEGAELRVTAKDMTPSSSFRIEPGSGVALLTVNGGSS
                     AARALGRLGLTIGVPLSLVGMAGFGYGHFDERKGLETAGAISLAAGAALVLVALPLLV
                     SGSTSVRNAKGDLIAAGTKAPTF"
     gene            1531..2271
                     /locus_tag="VFQ35_28105"
     CDS             1531..2271
                     /locus_tag="VFQ35_28105"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9934613.1"
                     /translation="MRYTLRYQQRDIDLDEGEFVVGRAISCQLALDDPLVSRTHARFV
                     VSDAGVTLEDLGSRNGVKVNGQRISGPSELHHGDHVAIGSQELVLLTRRDLSVDTLVQ
                     MPTNERDPFGLMGALADKALGLGRGDEAERLIGQQLEQLLSDVETGRNPSPESLERAA
                     TYALRLASAMQSARWTDFLFKMYAAQKRLCPASTVDELYTVLRKVHRPSPNSLRNYLN
                     VLRSLELGPADRFVLNRLEGLERLLSAR"
     gene            2565..>2874
                     /locus_tag="VFQ35_28110"
     CDS             2565..>2874
                     /locus_tag="VFQ35_28110"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9934614.1"
                     /translation="MEEDRHANHTPPAAGEPVPQSAGPRYWLDYGGRTIELRPGVISL
                     GRSSACHIVLDDSLVSRRHAELLVNAGAVTVQDCGSVNGVFVNGKRVVGSERLKDGDQ
                     V"
BASE COUNT          488 a          894 c          972 g          520 t
ORIGIN      
        1 caacacgatc ttcggctcgc agtggcacga gatcccgaac accggcagcg tgccgctcgt
       61 cgccgacatg tcgagcgaca tcatgtggcg cccgatggac gtctcgaagt tcggcctgat
      121 ctacgccggc gcgcagaaga acctcggccc gagcgggctc gtcgtcgtca tcgtgcgcaa
      181 ggatctcgtc gcctcggggc gccaggacat tccggtgatc ttccagtacc ggacgcactc
      241 cgagaacaac tcgctctaca acacgccgcc gacgttctcg gtgtacgtgc tgcgcaacgt
      301 gctcgacgag gtgaagaaag ctggcgggct cgcggcgatg gagaagatca accgcgagaa
      361 ggcgaagctc ttgtacgacg cgatcgacga gcgctcggac gtcttcagcg cgccggtcga
      421 gaaagactcg cgctcggtga tgaacgtggt cttcacgctg aagacccccg aggccgaggc
      481 cgagttcctc gccgaggcgc agaagcgcgg catgatcggc ttgaagggcc accgttcggt
      541 cggcggcatg cgcgcgtcga tctacaacgc ggttccggtc gaggccgtga aggcgctcgc
      601 ggatctggtt cgttcgttca agaagtgagc tgagtgaatc gtggcgggcg cggcggctgg
      661 ctgtcgcgcc cgtctccgct cagcagttag aacgtcggcg ctttggttcc cgccgcgatg
      721 aggtcgcctt tcgcgtttct gacggaagtg ctcccgctga cgagcaacgg caacgcgacg
      781 agcacgagcg ccgcgccggc tgcgagtgag atcgcccctg cggtttcgag ccctttgcgc
      841 tcgtcgaaat gaccgtagcc aaagccggcc atcccaacga gcgagagcgg aacgccgatg
      901 gtcaacccca ggcggccgag cgcccgcgcc gcggaagaac cgccgttgac ggtgagcagg
      961 gcaacgccgc tgccgggctc gattctgaaa gagctgctcg gtgtcatgtc tttcgccgtc
     1021 acgcgcagct cggcaccctc gacctcgacc cattcaccgc atggggcttt gcagcgcggc
     1081 tgccaagggt ctccgctccc gaaaggacgc gcttcgagcc aggcctccgg tcgacttacg
     1141 gcgaagcgga cctgagcacg acctgcgctt gaggacgggg gatgggaggg cgcgacgcgc
     1201 gtttcggccg ggggagcaac tggcggtgtt ggcgcgctag gaggggctgc attggccggc
     1261 gcaggtggtt cggacgtagg tgtcggtgcg tcgagcttct cctcgtgcgg ctcggctggc
     1321 gtgctggcgt tgggttcggc ttgcgccatg ccagccacca aggtgaggag aagtacgacc
     1381 gactttgcgc agcgcatgcg cggatgcaac acgccgcgcc caggctgagc aagtttgttt
     1441 gctagattga cgatcggcgc cgccgcgtcg tgtccccgtc acgagcgttc gagttgccta
     1501 ccggcgctta catcgagcgc cggctccccg atgagataca cgctccgcta tcagcagcgc
     1561 gatattgacc tcgatgaggg tgagttcgta gtggggcgtg ccatcagctg tcagctcgcg
     1621 ctcgacgacc cactcgtctc tcgaactcat gcgcgtttcg tggtttcgga tgcgggcgta
     1681 acgttggaag atctgggcag tcgtaacgga gtgaaggtca atggccagcg gatctctggc
     1741 ccgagcgagc tccatcacgg agaccacgtg gcgatcggca gccaagagct cgtgcttctc
     1801 acccgtcgcg acctctccgt cgacactctc gtacagatgc cgacgaacga acgcgatccg
     1861 ttcgggttga tgggtgcgct cgcggacaaa gccctcggct tggggcgtgg cgacgaggcg
     1921 gagcgcttga tcggtcagca gctcgagcaa ttgctcagcg acgtcgaaac gggccgaaat
     1981 ccgagccccg agagcctgga gcgcgcggcg acgtacgcat tgcggctcgc gtccgcgatg
     2041 cagagcgctc gctggaccga ctttctcttc aagatgtacg ccgcgcagaa acgcctctgc
     2101 ccagcgtcga ccgtggacga gctctatacg gtgttgcgca aagtgcatcg gccgagcccg
     2161 aactcgctac gcaactactt gaacgtgttg cggtcgctcg aactcggccc cgccgatcgg
     2221 ttcgttctca atcgtctcga ggggctcgag cgcttgctga gtgctcgata ggccagttct
     2281 tttgcgcttt ccgaaaccga attcgcgggt ggcatcgcac gtgtgagcgc tcccatgggc
     2341 gggcgccgaa gctggctctc tcttttgtga gccggttgcg cgctgagccg gatcgccgag
     2401 ccggctcttc ggcggcgtca catggtcgaa cggcactggt tcaggacgtc gttcagccat
     2461 ggcttcgcgg cgtcgcggga tgcttccttt tcctacacgc gaacgagcgc cgaccagaat
     2521 tcgcgattgc cgctcgagaa aggggcggtt agcctggagg caaggtggaa gaagaccgcc
     2581 acgcgaatca cacgccgccc gcggctggtg agcccgttcc ccagtcggcg gggccgcgct
     2641 attggctcga ctatgggggg agaaccatcg agctacgccc gggagttatc tccttgggaa
     2701 ggagctccgc ctgtcacatc gtgctcgacg actcgttggt ctcgcggcgc cacgccgagc
     2761 tcttggtcaa tgccggcgcg gtcactgtgc aggattgcgg cagcgtcaac ggcgtgttcg
     2821 tgaatggtaa acgcgtcgtc gggagcgagc gattgaagga cggcgaccag gttc
//
LOCUS       DASTKS010001313         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1106167, whole genome shotgun sequence.
ACCESSION   DASTKS010001313 DASTKS010000000
VERSION     DASTKS010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106167"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFQ35_28115"
     CDS             complement(<1..1151)
                     /locus_tag="VFQ35_28115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9934615.1"
                     /translation="MIRQIIRFSAENRYLVIAATIVALVMAWWSMRTIPLDALPDLAD
                     TQVIVYSRWDRSPDIIEDQVTYPITSALLGAPKVKAIRGFSDFGFSYVYAIFEDGTDM
                     YWARTRVLEYLSKITPQLPQGVKVELGPDASSVGWVFQYALVDKSGKHASDELRSYQD
                     WFLRYAVQSVPGVSEVATVGGQVRQYQITVNPNSLAAYRLPLESVIDAVRKGNNDVGG
                     RLVELSGREYMVRGRGYVKSTRDIEEIVLRAEGGTPVRIKDIATVALGPEMRRGVADY
                     NGQGDVVGGIVVMRQGENALNVIQRVKAKLEELKPALPAGVEVVTTYDRSELIERAID
                     TVKHKLVEEIIIVSLIILVFLWHFPSSLVPIVTIPVSVVLAFIPMKLMGL"
     gene            complement(1148..2743)
                     /locus_tag="VFQ35_28120"
     CDS             complement(1148..2743)
                     /locus_tag="VFQ35_28120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9934616.1"
                     /translation="MTSDASEGSEALGAQRPESAPPPQAHVERAAAARRFGVGTLVAA
                     MLATAAVSSVASIVTFRALSARSASGTHSDAEAPLYQCPMHPSIIQDHPGDCPICGMK
                     LVRVASATLHKSDSSTALATKAEPSAAPAVPGMAAVEIDANRQQLIGLKTAEVLEGSV
                     GGAWRTVGRVAVDETRVRHVNLKVAGFVEHVYVDYVGKKVNKGDPLFSIYSPDLLAAQ
                     QEYLVASKLRTDLAKLAQNDGATDTALLAAARRKLELWDIPTADLEKLERTREATRTL
                     TLRSPVSGVVTKKDVVDGMKLDAGAMPYEIVDLSTVWVLADVYESELRFVKEGMAATL
                     TLAAFPNREFKGKVVFLDPLLDAQTRTVKVRLNFPNPSGELRPEMFGEVVLLGPPRQA
                     LRIPTDAVIDSGTERVVFVAVGEGKFEPRPVKVGESDGANIEIVSGLKAGERVVVRAN
                     FLVDSESRLRASLQAIAPASSANAPESARVLPQPTSPSPNRAEPPSPAAAPPRDTSGT
                     QSSPTPHSGHDHGAHGDHSGHGQ"
     gene            complement(2750..3139)
                     /locus_tag="VFQ35_28125"
     CDS             complement(2750..3139)
                     /locus_tag="VFQ35_28125"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9934617.1"
                     /translation="MHRPNVFVSVLLLSVVAFAGGCNRSDASQTKSEAAAVAKPDANG
                     RVNIEVTSDGFTPALTHVKVGQPVTLVVTRKVEKTCATEIVMDSEGINRPLPLNEPVE
                     VKFTPSKAGKIPFSCAMHMVSGELVAE"
     gene            3797..>4497
                     /locus_tag="VFQ35_28130"
     CDS             3797..>4497
                     /locus_tag="VFQ35_28130"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9934618.1"
                     /translation="MNRAVLFWPEHWQQVLGAFGLALLSAGACSVPFVLGHGRRAGQV
                     ALVVLAAFAAGELRRLVLRREYRVELRATPASGASHLNTTRDLRIQHYALPLAAPLTK
                     KLRLVQITDVHVTEQFPFDYYERLSALVASRKPDVLALTGDLLSRRERLPLLVRWLRT
                     LPEAPLGRYAVLGNHEYWAHAEDEVRSALAAAHVSVIAGECRTLSDAGAPGGLRVCGT
                     EAPWGPALKASALST"
BASE COUNT          723 a         1468 c         1483 g          823 t
ORIGIN      
        1 agccccatca gcttcatcgg gatgaacgcc agcacgaccg aaaccgggat cgtgacgatc
       61 ggcacgagcg acgaagggaa gtgccagaga aacaccaaaa tgatgagtga aacgatgatg
      121 atctcttcga cgagcttgtg cttcaccgtg tcgatcgcgc gctcgatgag ctcggagcgg
      181 tcgtaggtgg tgaccacctc cacgccggca ggcagcgccg gcttcagctc ttcgagcttg
      241 gctttgactc gctggatgac gttgagcgcg ttttcgccct ggcgcatcac gacgatgcca
      301 ccgaccacgt cgccttggcc gttgtagtcg gcgacgccgc ggcgcatttc gggcccgagc
      361 gccaccgtcg cgatgtcctt gattcgtacc ggagtgccgc cctccgcgcg cagcacgatt
      421 tcttcgatgt cgcgcgtcga cttcacgtag ccacggcctc gcaccatgta ctcgcgaccg
      481 gagagctcga ccaagcgccc gccgacgtcg ttgttcccct tgcgaaccgc gtcgatcacg
      541 ctttcgagcg gcagccgata cgccgcgagc gagttcgggt tcaccgtgat ttgatattgg
      601 cgcacctgac cgccgacggt cgccacctcc gagacgcccg gcacgctctg caccgcgtag
      661 cgcaggaacc aatcctgata cgatctcagc tcgtcggagg cgtgcttgcc gctcttatcg
      721 acgagcgcgt attggaacac ccagccgacg ctcgaggcgt ctggcccgag ctcgaccttg
      781 accccttgag gtagctgcgg ggttatcttc gataggtact cgagcacgcg ggttcgcgcc
      841 cagtacatgt cggtcccatc ttcgaagatg gcgtagacgt agctgaaccc gaaatccgaa
      901 aagccgcgga tcgctttcac cttcggcgcg ccgagcagcg ccgacgtgat cgggtacgtc
      961 acctggtctt ctatgatgtc ggggctgcga tcccaacggc tgtagacgat gacctgggtg
     1021 tcggcgaggt cgggcagagc gtcgaggggg atggtgcgca tcgaccacca cgccatgacg
     1081 agcgctacga tggtcgcagc aatgaccaag tagcggttct ccgcggagaa tcggatgatc
     1141 tgccggatca ttgtccatgt ccgctgtggt cgccgtgcgc gccgtggtca tggccgctgt
     1201 gcggcgtggg cgaactttgg gtgccagagg tgtcgcgcgg cggtgcggcg gcaggagagg
     1261 ggggctccgc gcggttcggc gacggcgagg tcggttgggg caggacgcga gcgctttccg
     1321 gcgcattcgc cgacgaggcg ggcgcgatcg cttgaagcga agcgcgcagg cgcgactcgg
     1381 agtcgaccag gaagtttgcg cgcaccacga cgcgctcgcc cgccttcagc ccactcacga
     1441 tctcgatgtt ggcgccgtcg ctctccccga ccttcaccgg acgcggctcg aatttgcctt
     1501 cgccaaccgc gacgaacacc actcgctccg ttcccgagtc gatcaccgcg tcggtcggta
     1561 tccggagcgc ctgccgaggt ggccccagca agactacttc tccgaacatc tccgggcgca
     1621 gctcgccgct cgggttcgga aaattgagcc ggactttcac cgtgcgcgtt tgcgcatcga
     1681 gcagcggatc caaaaacacg accttgcctt tgaactcgcg gttagggaaa gcggcgagcg
     1741 tgagcgtcgc ggccatcccc tcttttacga agcggagctc actctcgtaa acgtcagcga
     1801 gcacccacac cgtggacaag tcgacgatct cgtagggcat cgcgcccgcg tcgagcttca
     1861 ttccgtcgac gacgtccttc ttggtgacga cgccggagac aggcgagcgc aacgtcagcg
     1921 tccgcgtcgc ttcacgtgtc cgctcgagct tctccagatc ggccgtcgga atgtcccaga
     1981 gctcgagctt gcgtcgggcc gccgccaaca gagcggtatc cgtggcgccg tcgttttgcg
     2041 cgagcttggc gagatcggtg cggagcttgg aggcaaccaa atactcttgc tgcgccgcca
     2101 agaggtctgg gctgtagatc gagaacagag ggtcgccctt gttgaccttc tttcccacgt
     2161 agtcgacgta aacgtgctcg acgaagccag cgaccttcag gttgacgtgg cgaacgcgtg
     2221 tctcgtcgac cgcgacgcga cccaccgtgc gccacgcgcc gccgaccgag ccttcgagca
     2281 cctcggccgt cttgagtccg atgagctgct gacgattcgc gtcgatttcg accgccgcca
     2341 tccccggtac cgccggtgct gcgctcggct cggccttggt cgcgagcgcc gtgctcgagt
     2401 cgctcttgtg aagcgtcgcg gatgcgaccc ggaccagctt catgccgcaa atcgggcagt
     2461 ccccggggtg atcttggatg atcgaagggt gcattgggca ctggtacagc ggcgcctcgg
     2521 catcgctgtg cgtccccgat gcgctgcgcg cgctcagcgc tcgaaacgtg acgatggacg
     2581 cgaccgacga aaccgctgcg gtcgcgagca ttgctgccac gagagttccg actccgaagc
     2641 gacgcgccgc cgccgctcgc tcgacgtggg cttggggtgg cggggccgac tcggggcgct
     2701 gcgcgccgag cgcctcagaa ccttcggacg catcggaagt catgaacgcc tactccgcga
     2761 cgagctcgcc tgaaaccatg tgcatcgcgc acgagaacgg aatcttcccg gctttgctcg
     2821 gggtgaactt cacctcgacg ggctcgttca acggcagcgg ccggttgata ccctccgagt
     2881 ccatcacgat ctcggtggcg cacgtctttt cgactttgcg cgttacgacc agcgtcaccg
     2941 gctgaccgac tttcacgtgt gtgagcgcgg gcgtgaaacc atcgctcgtc acttcgatgt
     3001 tcactcgccc gttcgcgtcg ggcttggcta cggcggcggc ttcggacttg gtctgcgaag
     3061 cgtcgctccg attgcagccc ccggcgaaag ccacgaccga caggagaaga accgaaacga
     3121 agacgttggg acgatgcatg acaggtgctc cgatgtgagt ctcgaacgaa aagccggttg
     3181 ccgaggatag gcgggaccgc gcccaagcgt gaccgccggc acggatgccc cactgctttc
     3241 cgccccgcgt tttcgagcta cgcaagccac gtgccactgc tcgcgggaag gtcgcatctc
     3301 gctgcggtcg tctcggcggt gccgtcgcgg tcgcctcggc ggtgccgctg cccaacgggc
     3361 gccgtagccg gaacgccgcg ggaaacccac gcaaagatcg cgccgccgcg caatgcgtgc
     3421 aggggcgcac gccctgcgcc caccgcgtcc gtcgagtgcg gggacgctcg ccaccacatc
     3481 cgcgcgaccc gcgtactccc cgcctcgagt ggctactcac cgcgcgccct tcgtcgtgtg
     3541 aagaacgagc tctcggcatt ctgagccagc tgggtgcgaa gcggcgcggc tcctcctcgc
     3601 gcggtgtcgg ggcacttcga gctcgcaatc gcgcgcgacg ctttcggttt tgactgatgc
     3661 tttcggtcgg tccacggtag ccgcggtgtt ttctggggcc gaacggggca ggcggaaggg
     3721 gtggtgggaa gtcgcgctcg gtcgcggcgc ttgggcgtgc gtgttcttgg cggcagcggg
     3781 cctcagcgcg tacgcaatga accgcgccgt tttgttttgg ccggaacact ggcagcaagt
     3841 gctcggtgcc tttggcctcg cgctcctcag cgcgggcgcg tgcagcgtgc cgttcgtgtt
     3901 ggggcacggg agacgagccg gtcaggttgc gttggtcgtt ttggccgcgt tcgcggcggg
     3961 ggagcttcgt cgcctcgttc tacggcgcga atatcgagtc gaactgcgcg cgacgcccgc
     4021 gagcggagct tcgcacctga acacgacgcg tgatttgcgg atccaacatt acgccttgcc
     4081 gctcgctgcg cctctgacca agaagcttcg cctggtgcag atcacggacg tgcacgtgac
     4141 cgagcaattc cctttcgact actacgagcg cctcagcgcg ctcgtagcga gcagaaagcc
     4201 ggacgtcttg gcgctcacgg gagatctctt gtcgcgtcgc gaaagactgc cattgctggt
     4261 gcggtggcta cggacgcttc cagaagcccc gctcggccgt tatgcggtgc tcggcaatca
     4321 cgagtactgg gctcacgccg aagacgaggt gcggagcgcg ctcgcggcgg ctcacgtctc
     4381 tgtcatcgcg ggtgagtgcc gcactctttc ggacgcgggc gctccgggcg ggcttcgcgt
     4441 gtgcgggacc gaggcgccct ggggccccgc tttgaaggcg agcgcgttga gcacgga
//
LOCUS       DASTKS010001314         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1331040, whole genome shotgun sequence.
ACCESSION   DASTKS010001314 DASTKS010000000
VERSION     DASTKS010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331040"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFQ35_28135"
     CDS             complement(<1..247)
                     /locus_tag="VFQ35_28135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934619.1"
                     /translation="MRSLASMTSSGPWNVLKPSRWPRAAVGPALALALSSLASGCNHQ
                     KAAELPAGPATARNSDRSGRQNVSVTIYNSNFGLVRET"
     gene            complement(299..799)
                     /locus_tag="VFQ35_28140"
     CDS             complement(299..799)
                     /locus_tag="VFQ35_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9934620.1"
                     /translation="MRRRTPQTPPRQRVLALLKKELIMNTSYSALRAQSGPLVLRLAL
                     GIVFLAHAYAKAFSFTFAGTEAFFRANGFPGWTVYPVFVAELLGGLALVLGVRTRVVA
                     LALIPVMLGALRVHLGNGWMFTSTGGGWEYPLFLIVALGAQGLLGAGAYSFDALPTSR
                     HKAVAA"
     gene            842..1756
                     /locus_tag="VFQ35_28145"
     CDS             842..1756
                     /locus_tag="VFQ35_28145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9934621.1"
                     /translation="MPDWDDLRYALAVADAGSLAGAARLLRVDHTTVLRRIAGLERAL
                     NARLFERLPTGYALTPAGEELVAAARRVSETVTEVERRIAGEDLRLTGVLRLATSDTL
                     MNTVLPEALAGFAAAHPSVTLELSTSTSMVNLTKREADVALRPARKIPENLVGRRIAQ
                     VAIALYAAPSYLQHSPPRKALSKHVWIGLDDSLSETSVAQWMARELKDVSIRTRTDTI
                     SAAARAAVAGLGVAALPCYLGDLTPRLRRLGKPIAAMSTDLWLVTHEDLQKTARVRAI
                     MDWLSNVLTQERDLLEGGRPHRRKHAGS"
     gene            1968..3113
                     /locus_tag="VFQ35_28150"
     CDS             1968..3113
                     /locus_tag="VFQ35_28150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934622.1"
                     /translation="MREVTSRSILGMAASAIMALCCLTRCSGEEGCSGTACAAGGALG
                     YGGKNGSGGASGGTSSSGGTSAFGGSAGGDDAGSGGEIGASAGAGGEGGGTCGECAPY
                     EFCRNGSCVACSDVRGATFGSPQRLGVTGRYPRPTIDDGLFYVATNAIFFAAADGTPP
                     IAITDGIAGKDSAPLYVSGLDALLPNQNFFFLRTDVNGAALRAGYFLNASTPSLTKVG
                     DAPALFASEDGFNYSLAISLTASRAYWRTTRSASPGIVTASLASDGTGAAPVDVQVSV
                     GSRTCPADETDPTAWVTPDGALLLFRQSIVNESCEHPLGFSTDLYALPLNPATGVPLG
                     PASPLGVWLGGNARDSDPTFGPDCSLYFSSDVGANGIFELYRAIRDH"
BASE COUNT          577 a         1060 c         1070 g          531 t
ORIGIN      
        1 gggtttcccg caccaggccg aagttcgagt tataaatagt gaccgagaca ttttggcgcc
       61 cgctccggtc cgagttgcga gccgtggccg gcccagccgg gagctcggcc gccttttggt
      121 gattgcagcc cgaggcgagc gacgaaaggg cgagggcgag cgccgggccc accgcggctc
      181 ttggccagcg cgacggtttg aggacattcc aaggaccgct cgacgtcatg ctggccagag
      241 aacgcatcgg gtgggcgata cttacagcag cgcgtgccct tcgcaatgcc ggttgccgtc
      301 aggccgccac cgccttgtgg cgagaggtag gcaaagcgtc gaatgaatac gccccggccc
      361 cgagcagccc ctgcgcgccg agagcgacga tcagaaatag tgggtactcc caacccccgc
      421 cggtgctcgt gaacatccag ccgttcccta ggtggacccg caacgcgccg agcatcactg
      481 gaatcagtgc gagcgcgacc acgcgcgtac gcaccccgag gacgagcgcc agccctccca
      541 ggagctccgc gacgaagacc gggtagaccg tccatcccgg aaagccgttg gcgcggaaga
      601 atgcctcggt tcccgcgaaa gtgaacgaga acgccttcgc gtacgcgtgg gccagaaaga
      661 cgatgccgag agcgagcctg agaaccagtg ggcccgattg agcgcgcagg gcgctgtaag
      721 acgtgttcat gatgagctcc ttcttgagca gcgcgagcac gcgctgacga ggaggagttt
      781 ggggtgtgcg ccggcgcata gacaggtgac aactttgcag ccagactctg caaaaatgca
      841 catgcctgac tgggatgacc ttcgctacgc gctcgcggtt gcagacgcag gctcgttggc
      901 aggggccgcg cggctcctgc gcgtcgacca caccaccgtg ctgcgtcgta tcgcgggtct
      961 cgagagagcg ctcaatgcca ggctattcga gcggctcccg accggctatg cgctgacccc
     1021 ggccggagaa gagctcgtgg cggccgcgag acgagtgagc gaaaccgtga ccgaggtgga
     1081 gcgacgcatc gcgggagagg atctccgcct gaccggggtg ctgcgcctcg ctacctcgga
     1141 cacgctgatg aacacggtgc tgcccgaggc tctggcgggt ttcgcggccg cgcacccaag
     1201 cgtcacactc gagctcagca cctcgacatc gatggtgaat ctcaccaagc gggaagccga
     1261 cgtagctctg cgccccgctc gcaagattcc ggagaacttg gtgggacgac gcatcgctca
     1321 ggtcgcgatc gcgttgtacg ccgccccgag ttacctgcaa cactcgccgc cgcgcaaagc
     1381 gctctcgaag catgtctgga tcggcctcga cgacagcctc tccgagacct ccgtcgcaca
     1441 gtggatggcg cgggagctga aggacgtatc cattcgcacg aggaccgaca ccatttccgc
     1501 ggcagcgagg gccgcggtcg ctggtctcgg cgtcgccgcc ctgccctgct atctaggcga
     1561 cctcacgccc aggcttcggc gtttgggcaa gcccatcgcg gcgatgagca cggatctgtg
     1621 gctcgtcacg cacgaggacc ttcaaaaaac cgctcgggtc cgggcgatta tggattggct
     1681 ttcgaacgta ctcacccaag agcgcgactt gctcgaaggg gggcgaccgc atcggcggaa
     1741 gcacgccgga agctgaatcc ggagggaggt cgagcgaatc cggagggagg ttcgagcgaa
     1801 ctgaaaccgc cgttccaagc gtcggcccca cggcccatag catcgttttg cgctcacccg
     1861 tgacgcgtgg cttgatctcc gaggaaacca actcttctcg gttggtttca atctgccgac
     1921 acgctaaggg tttcgttcgc atctcgaacg atcatcgtgg aagttcggtg agggaagtca
     1981 cgtctcgttc cattctcggc atggccgctt cggcgatcat ggcgctgtgc tgcttgacgc
     2041 ggtgctcggg cgaggagggc tgcagcggaa ccgcttgcgc tgctggtggc gcgctgggat
     2101 acggcggcaa aaacggcagc ggaggtgctt cgggtggaac gtcgagttct ggcggcacat
     2161 cggccttcgg cggttcggcg ggcggcgacg acgcgggcag tggtggagaa atcggtgcaa
     2221 gcgcgggcgc cggcggcgag ggcggcggaa cctgcggcga gtgcgcgccg tacgagttct
     2281 gtcgaaacgg cagctgcgtg gcgtgcagcg acgtgcgagg cgcaacgttc ggcagcccgc
     2341 agcgtctcgg cgtgaccggc cgctatccgc gccccacgat cgacgatggg ctcttctacg
     2401 tcgcaaccaa cgcgatcttc ttcgctgcgg cagacggaac acccccaatc gcaatcacgg
     2461 acggcatcgc cggtaaagac agcgccccgc tctacgtcag cgggctcgac gcgctgcttc
     2521 cgaaccagaa cttcttcttc ttgcggaccg acgtgaacgg ggcagcgctt cgcgccggct
     2581 acttcctgaa cgcctcgacc cccagcttga cgaaggtcgg cgatgctccc gccctcttcg
     2641 cgtcggagga cggtttcaac tatagcctcg cgatttcgct gaccgcgtcg cgagcctact
     2701 ggcgcacgac ccggagcgcg tcgccaggca tcgtcaccgc atcgctcgcg agcgatggaa
     2761 ccggcgctgc accggtcgac gttcaggtca gcgtcggctc tcgaacatgc cccgccgacg
     2821 agacggatcc gacggcctgg gtgacgcccg acggtgcgct tctcttgttc cgccaaagca
     2881 tcgtcaacga aagctgcgag cacccactcg gcttctcgac ggatctttac gcgctgccgc
     2941 tgaatccggc gacgggcgtg ccgctcggcc ccgcctcacc gctcggagtc tggttgggag
     3001 gaaacgcgcg cgatagcgac cccacgttcg gcccggattg ctcgctctac ttctcgagcg
     3061 acgtgggcgc gaacggcatc ttcgagctgt accgcgccat tcgcgaccac tgaaaacacg
     3121 aacgggaagg taccgtctct ggcccttccc gtcgtgtcgg cggggctcgc ctcacttctc
     3181 agccgtggga catccacgga gcgggaccgc tcggccccaa gtcgctgagc gcagcgtc
//
LOCUS       DASTKS010001315         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_2001880, whole genome shotgun sequence.
ACCESSION   DASTKS010001315 DASTKS010000000
VERSION     DASTKS010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001880"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFQ35_28155"
     CDS             <1..705
                     /locus_tag="VFQ35_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934623.1"
                     /translation="RVQSDTTEGAVSEGALALRVLELFRARALRLPAEPKPRPPEPPK
                     PPPRALPPAAPAEKGATSTRERRAEVWVGAGPTFAGGAPRPLVDASLGARVGLLRPLS
                     LEATAALSVAPVQFETNAGEVEISARRLTLHLMGDLFRSSNVRLAFGAGGGAIWVSES
                     ARPLSGYEGHADSAYLGLASLRASATLESGALAFGLRFEPGLTLPRASIRSSGEELAH
                     LGNTWASLTASIGWSP"
     gene            complement(716..1069)
                     /locus_tag="VFQ35_28160"
     CDS             complement(716..1069)
                     /locus_tag="VFQ35_28160"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9934624.1"
                     /translation="MAKWLLAWLIQSAAVWVTAAVLPGFELRPGFWPAVRVAAVIGIL
                     NFLLGKLLFVVLGISTLGLGFLFAFITRWIVAALLLKLAAALSSSLRIASFGRALIGA
                     LLISVVGTVLERLLT"
     gene            complement(1079..2050)
                     /locus_tag="VFQ35_28165"
     CDS             complement(1079..2050)
                     /locus_tag="VFQ35_28165"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9934625.1"
                     /translation="MSLVPRVPSPIRHLPALSGSGGELFIKDDGELHSLYGGNKVRKL
                     ALIFADARERGSRRIVTFGAAGSHHVLTAGLFAPAFGLRAAALLAPQPKTPHAEMVLR
                     AILATGLDVTPIERWELAPLAFARSWQRGDYVVGPGGSGLLGTLAYAAAVEELEQQIA
                     RGLLPMPDVVVTAVGSGGTAAGLLSGIVARKLPIRVVGVQVAGGRATKALVLGLATRA
                     LVAKGISPRGLTRHFELTRAELGRGYGYASPGGLRAREAAATQNIRVEPTYTEKALAH
                     ALALTHDASRAKRQVILYWHTLSAREPPNPVEGPLAARFEALLSSMG"
     gene            2169..>3194
                     /gene="hflX"
                     /locus_tag="VFQ35_28170"
     CDS             2169..>3194
                     /gene="hflX"
                     /locus_tag="VFQ35_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9934626.1"
                     /translation="MTSERPKALLVGVRLPGVDIPEFRSSLGELKRLVTTLGYDVVGE
                     VTQHREALAAAAVVGEGKLEELCALTGGSGSTHVVPRNKDKARLRREASQEGANHLDE
                     DLDEDEDEDEATSAGEPPIALVAVDHEVSPSQLRNLVRATGTEVFDRTGVIIEIFHRH
                     AKSREARLQVEIARLTYTAPRLRESPGGKERQRGRGAGEAALELDRRKIRDRIAELRE
                     ELAGIQREQDVRRLHRKHARRVALVGYTNAGKSSLMRALTGSAVYVENKLFATLDTTV
                     RALHPEAKPRILVSDTVGFIKKLPHDLVASFKSTLDEAHEASLLLHVVDAADPSWESQ
                     LEVTREVL"
BASE COUNT          593 a         1125 c         1032 g          444 t
ORIGIN      
        1 cgcgtccaga gcgataccac cgaaggcgcg gttagcgagg gcgcgctcgc gctacgcgtg
       61 ttggagctct tccgagcccg cgcattgcgc ttgcccgccg agcccaagcc gcgccctccc
      121 gaaccgccaa agcccccgcc gcgcgcgcta ccgccagcgg ctccggcgga aaaaggcgcc
      181 acttccaccc gcgagcgtcg cgccgaagtc tgggtcggcg ccgggcccac gtttgcgggg
      241 ggagccccga ggccactcgt cgacgcgtca ctcggggcgc gggtcggtct gctccgcccg
      301 ctttctctcg aggccacggc tgcactgtcc gtagcgcccg tgcaattcga gacgaacgcc
      361 ggcgaagtgg aaatctcggc gcgtcgcctc accttgcatc tgatgggcga tctgttccgc
      421 tcgtccaacg tgcgcttggc atttggagcg ggcggcggcg cgatctgggt cagcgaatca
      481 gcgcgccccc tgagcggcta cgaaggccac gccgactcgg cttatctggg cttggcctcg
      541 ctgcgcgcga gcgcaacgct cgagagcggc gcgctcgctt tcggcctccg attcgagccg
      601 gggttgacgt taccgcgagc cagcattcgc tcgagcggtg aagagctcgc gcacctcggc
      661 aacacgtggg cgagccttac cgcatcgatc ggttggtcgc cgtaaaacaa caggctcagg
      721 tcagtaggcg ctccagcacc gtcccgacga cgctgatcag caaggctccg atcaaagccc
      781 gaccgaacga ggcgatgcga aggctgctcg agagcgcggc cgcgagcttc aacaaaagcg
      841 ccgcgactat ccaacgggtg atgaacgcga agaggaaacc gagccccaac gtcgagatgc
      901 cgaggacgac gaagagcagc ttgccgagta ggaagttgag aatgccaatg acggcggcca
      961 cgcgcacggc gggccaaaac cctggtcgaa gctcgaagcc aggaaggacc gccgccgtga
     1021 cccacactgc tgcgctctga atcagccacg ccaatagcca cttcgccatg gtgcgagtct
     1081 agcccatact cgacagcaac gcttcgaacc tggctgcgag cggtccttca accggattcg
     1141 gaggctcacg ggcagagagc gtgtgccagt acaagatcac ctgtcgcttc gcgcggctcg
     1201 cgtcgtgagt cagcgcgagc gcgtgcgcga gcgctttctc ggtgtacgtc ggttcgacgc
     1261 gaatgttctg cgtcgccgcc gcctcccgcg ctcgaagccc acctgggctc gcgtaaccgt
     1321 agccgcgccc gagctccgcg cgagtgagct cgaagtgccg cgtgagcccc cgaggagaaa
     1381 tccccttcgc gacgagcgcg cgcgtcgcga ggcccaaaac cagcgccttc gtcgctcggc
     1441 cgcccgcgac ctgaacgccg accacccgaa tcggaagctt tcgcgccacg atgccgctga
     1501 gcaaccccgc cgcggttccc cccgagccga ccgcggtgac gacgacatcc ggcatcggta
     1561 agagccctcg cgctatctgc tgttcgagct cttctaccgc tgccgcgtag gcaagcgtcc
     1621 caaggagccc gctgccgccc ggcccgacga cgtagtcgcc gcgctgccac gagcgagcga
     1681 aagcgagcgg cgcgagctcc cagcgctcga tgggcgtgac atcgagaccc gtcgcaagaa
     1741 ttgcgcgcag caccatctcg gcgtgcggcg tcttgggctg cggcgcgagc aacgccgccg
     1801 cgcgaagacc gaacgcgggc gcgaagaggc ccgccgtcag cacgtgatga ctgcccgccg
     1861 cgccgaaagt aacgattcgg cggctccccc gttcgcgagc atcagcgaag atcagcgcga
     1921 gcttccgaac tttgttgccg ccatacagcg agtgcagctc gccatcgtct ttgatgaaga
     1981 gttcaccgcc cgagcccgag agcgctggca gatgacgtat tggagatggc acgcgcggga
     2041 cgagcgacac ggaggcgcgt atcgtgccca atcagcgcgc tccgaacaat agcggaagct
     2101 gtcgcaatcg ttaaacttta ttcggagcgg caagccgagc cgaaaaaggt ataacgcggg
     2161 acctcgaaat gacatcggaa cgaccgaaag cactgctcgt cggagtccgc ctgcctggcg
     2221 tcgacatccc cgagttccgc tccagtctcg gcgagctcaa acggctagtg actacgctgg
     2281 gctacgacgt ggtgggcgaa gtgacgcagc accgcgaagc cctagcggcg gccgcggtcg
     2341 tcggagaagg caagctcgaa gagctctgcg cgctgaccgg aggcagcggc agcacgcacg
     2401 tcgtcccgcg caacaaagac aaggctcgac tgcgtcgaga ggcttcgcag gaaggcgcca
     2461 accacctcga cgaagacctc gacgaagacg aagacgaaga cgaagcgacg agcgctggcg
     2521 agcctcccat cgcgctcgtg gccgtcgatc acgaggtctc gcccagccaa cttcgaaatc
     2581 tggtgcgcgc caccggtacg gaggtcttcg atcgcaccgg cgtcatcatc gagatcttcc
     2641 accgtcacgc gaagagccgc gaggcgcgcc tgcaagtcga gatcgcgcgg ctcacgtaca
     2701 ccgcgccgcg cctgcgcgag tcgcccggtg gcaaggagcg ccagcgcgga cgcggcgccg
     2761 gcgaggccgc cctcgagctc gatcgccgca agatccgcga ccggatcgcg gagctgcgcg
     2821 aggagctcgc cggcatccag cgcgagcagg acgtccgaag gctccaccgc aagcacgcgc
     2881 gccgcgtcgc gctcgtcggc tacacgaacg ccggcaagtc gtcgctcatg cgcgcgctca
     2941 ccggcagcgc cgtctacgtc gagaacaagc tcttcgcgac gctcgacacc accgtgcgcg
     3001 cgctgcaccc cgaggcgaag ccgcgcatcc tcgtgagcga caccgtcggc ttcatcaaga
     3061 agctgccgca cgatctcgtc gcgagcttca agtcgacgct cgacgaggcg cacgaggcct
     3121 cgctcctcct ccacgtcgtc gacgccgccg acccctcgtg ggagtcgcag ctcgaggtca
     3181 cgcgcgaggt cctc
//
LOCUS       DASTKS010001316         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_659200, whole genome shotgun sequence.
ACCESSION   DASTKS010001316 DASTKS010000000
VERSION     DASTKS010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659200"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..916)
                     /locus_tag="VFQ35_28175"
     CDS             complement(113..916)
                     /locus_tag="VFQ35_28175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9934627.1"
                     /translation="MKKADFTGKWVLITGASSGLGEEFARQLAHRGANLILTARSEDK
                     LRYFAHDLARVNGVSTRFVVADLAEPLGITALLEGVDALHVPVDHVINNAGVGSTGPF
                     AETDANAEMRMVRLNSEAVMRISRHFLPGMLARRSGGLIQVASTVAFQPMPFMTTYGA
                     TKAFVLSLSLALAEEVRDTGVRVLAFCPGPVVTGFQRAAGIQKGGLVRMAKLEAPRVV
                     RSALEAYDDGRFVVVPGAFNAVQTAAVRVLPRGIVLRATRWAMQGLGRA"
     gene            complement(913..2040)
                     /gene="aroC"
                     /locus_tag="VFQ35_28180"
     CDS             complement(913..2040)
                     /gene="aroC"
                     /locus_tag="VFQ35_28180"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9934628.1"
                     /translation="MPGNSFGQAFCITTAGESHGPGNVVIIDGCPPGISLSVDDLRVD
                     LERRRPGQSKIVTQRAEADEPEILSGVFEGRTTGTSLAILVRNTDQKSRDYDDIKDKY
                     RPGHADHGYDAKYGFRDYRGGGRSSARETVSRVAAGVVAKKVLAELFGARVVGYVRQV
                     GPIVAEVPSPESVTLEQVERLPDGNPNIVRCPDPVSAARMVELIEAVRKDQDSIGGVS
                     EIVASGIPAGLGEPVFDKLKADLAKALFSLPAVMGVEYGVGFGCATLRGSETNDIYAV
                     REVGEPESIYTRTNRHGGMLGGISSGMPIVLRAAIKATSSLPREQESVNQQGEPATIR
                     TRGRHDPCLLPRFVPIGEAMVAIVLADHALRARAQAKLSAP"
     gene            complement(2079..3050)
                     /locus_tag="VFQ35_28185"
     CDS             complement(2079..3050)
                     /locus_tag="VFQ35_28185"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9934629.1"
                     /translation="MGLRNSADVRTLLWVAAAPVVAGIQYARPELVPYLSPLSCYLAL
                     SCGIFAHNHNHCPTFETKRGNQVFGTILSLFYGYPTFAWVPTHNLNHHKFVNRAGDAT
                     ITWRYTDKHNLLVALTYFFVSSYWQSEPIKDYIKKARDKNPKLYRTIITQYVVWIGTN
                     LALLALSIALNGVKTGLTTWLFATVIPSIFALWTIMWFNYEQHVHTDPWSKHDHSRNF
                     TGGLINFLLFNNGLHGVHHEHPGAHWSTLPGYFAKIENEIHPDLRNKNLAIYVLKTYF
                     LAIVMRRFESKQIGRAPFDPPTGEKVDLTTAEVIAAEAGTNAPMLAG"
     gene            3442..>3927
                     /locus_tag="VFQ35_28190"
     CDS             3442..>3927
                     /locus_tag="VFQ35_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9934630.1"
                     /translation="MNALSVLSEEEQLFFETVSAFAKERVKPRVAAMDEHARFDADLL
                     PLLFELGVMAVEVPAELGGAGSSFFNAILAVEALAEVDPSIAVLVDVQNTLVNNALLR
                     WGNAEQKARYFPKLASSWVGSYALSEASSGSDAFALKTRAERRGDRWLLRGRKLWITN
                     AN"
BASE COUNT          683 a         1223 c         1325 g          696 t
ORIGIN      
        1 gaacggcacg agcctgttgg ctaatcagca attgggtcag gtctggcgat aggacgcggc
       61 cgcgaacgaa tggacggagg acgacgacgc gcgaggctgg tgcttgctcg gttcaggcgc
      121 ggccgaggcc ctgcatggcc cagcgcgtcg cgcgcagcac gatgccgcgc ggaaggacgc
      181 gcaccgccgc cgtttgcacg gcgttgaacg cgccgggcac gaccacgaac ctgccgtcgt
      241 cgtaggcttc gagcgcgctc cggaccaccc gcggagcttc gagctttgcc atgcgcacga
      301 gcccgccctt ctgaatgcca gccgcgcgct gaaatccagt caccaccggg ccagggcaaa
      361 acgcgagtac ccgcacgccc gtgtcgcgca cctcttcggc gagggcgagc gataggctca
      421 gcacgaacgc cttcgtcgcg ccgtaagtcg tcatgaacgg catcggctga aacgcgacgg
      481 tcgacgcgac ctgaatcagt ccgccgctcc gccgggccag cattcctggc aaaaagtggc
      541 gcgaaatgcg catcacggct tcgctgttca ggcggaccat gcgcatctcg gcgttggcgt
      601 ccgtttcggc gaaaggacca gtcgagccga cgccggcgtt gttgatgacg tggtcgaccg
      661 gtacgtggag cgcgtcgacg ccttcgagca gggccgtgat gccgagcggc tccgcgagat
      721 cggcgacgac gaagcgcgtg gagacgccgt tcacccgcgc caagtcgtga gcgaagtaac
      781 ggagcttgtc ctccgagcgc gcggtgagga tcagatttgc gccacgatga gcgagctgcc
      841 gcgcgaactc ttcaccgagg cccgaggacg cgcccgtgat gagcacccat ttgcctgtga
      901 aatcggcctt cttcatggcg cgctcaattt cgcctgggca cgcgcgcgca gcgcgtggtc
      961 ggcgagcacg atcgcgacca tcgcctcgcc gattggaacg aagcgcggca acaggcacgg
     1021 gtcgtggcga ccgcgcgtgc ggatcgtcgc gggctcgccc tgctggttca cgctttcttg
     1081 ctcacggggc aagctgctgg tcgctttgat ggcggcgcgc agcacgatcg gcatgccgga
     1141 cgagatcccg ccgagcatgc ctccgtggcg attggtgcgc gtgtagatgc tctcgggctc
     1201 tccgacctcg cgcaccgcgt aaatgtcgtt ggtttcgctg ccgcgcaggg tggcgcagcc
     1261 gaagccgacg ccgtactcga cgcccatcac cgcgggcagg ctgaagagcg ccttggccaa
     1321 atcggccttc aacttgtcga acacgggttc accgaggccg gccggaatac cgctcgcaac
     1381 gatctcgctc acgccgccga ttgaatcctg atctttacgc accgcttcga tgagctcgac
     1441 catgcgcgcc gcactcaccg gatccggaca gcgcacgatg ttggggttac cgtcggggag
     1501 ccgctcgact tgttccagcg tgacgctctc gggcgacggc acctcggcca cgatggggcc
     1561 gacctggcgc acgtagccga ccacgcgcgc cccgaagagc tcggcgagga cctttttcgc
     1621 gacgacgccc gcggcgacgc gcgaaaccgt ttcacgcgcg ctcgagcgcc cgccgccgcg
     1681 gtagtcgcgg aagccgtact tcgcgtcgta gccgtgatcg gcgtgcccgg ggcggtactt
     1741 gtccttgatg tcgtcgtaat cgcggctctt ctggtccgtg ttgcgcacca gaatggcgag
     1801 gctcgtcccc gtggtccggc cctcgaacac gcccgagagg atctcgggct cgtcggcttc
     1861 ggcgcgctgc gtgacgatct tgctctgccc gggtcgccgc cgctcgaggt cgacgcgcag
     1921 gtcgtctacg gacagcgaaa tacctggcgg gcagccgtcg atgatgacga cgttgcccgg
     1981 cccgtggctt tcaccggcgg tggtgatgca gaaggcctga ccgaagctgt ttcccggcat
     2041 tcggcctgcc tcttaccacg tccgcacgga cgtggccgtc agcccgcgag catcggcgcg
     2101 ttcgtgccgg cctcggccgc aatcacctcg gccgtggtga ggtcgacctt ctcgcccgtc
     2161 ggcggatcga acggggcccg cccgatttgc ttgctctcga agcgccgcat gacgatggcg
     2221 aggaagtagg tcttcagcac gtagatcgcg aggttcttgt tgcggagatc ggggtggatc
     2281 tcgttttcga tcttcgcgaa gtagccgggc agggtgctcc agtgcgcgcc cgggtgttcg
     2341 tggtgcacgc cgtggagacc gttgttgaac aacaggaagt tgattagccc gccggtgaag
     2401 ttgcgggagt gatcatgctt cgaccacggg tcggtgtgca cgtgctgctc gtagttgaac
     2461 cacatgatgg tccagagcgc gaagatcgac gggatgacgg tcgcgaagag ccacgtggtg
     2521 aggcccgtct tcacgccgtt gagagcaatg ctcaacgcga gcagcgcgag gttggttcca
     2581 atccacacga cgtactgcgt gatgatggtt cggtagagct tcgggttctt gtcgcgagcc
     2641 ttcttgatgt agtccttgat tggctcgctc tgccaatagc tcgatacgaa gaagtacgtc
     2701 agcgcgacga gcaagttgtg cttgtcggtg taacgccagg tgatggtggc atcgcccgcg
     2761 cggttgacga acttgtgatg gttcaggttg tgagtgggga cccaggcgaa ggtcgggtat
     2821 ccgtagaaga gagacagaat cgtcccgaac acctgattgc cgcgcttggt ctcgaacgtc
     2881 gggcagtgat tgtgattgtg cgcgaagatg ccgcacgaaa gcgccaggta gcagctcaac
     2941 ggcgagagat atggaacgag ctcgggccga gcgtactgaa tgccggctac gaccggcgcc
     3001 gcagcgaccc agaggagtgt tcgaacgtct gcgctgtttc ggagtcccac aggcggcgat
     3061 gataggagac aatggtcgac gcgccagcaa aaagagcgaa agcgaatccg gctccagggg
     3121 aaacggtcag cgcgtcgcga gcgggtgtgt tccttcccgc cgggaatagt ctttgctccc
     3181 gggacgtgca cgctggcgct gcttcggggc gagacggccc caaagcaaag ctgaacgaca
     3241 gcccgagtcg tcgagtcagg tgttcgcagc acaagcggaa aacacggctc gcctgcgcat
     3301 gagccgaaag cgaatcaagg gctcccggag ccgagatgaa cccgccaaat tcccgcggat
     3361 ctcgccacga tcgagtgcgt tgctcactac gcacctcccg ccagtttcga accctggggc
     3421 gatcgtggca gagttttgga gatgaacgcg ctctcagttc tttcggaaga ggagcagctc
     3481 tttttcgaga ccgtatcggc attcgccaag gagcgcgtga agccgcgcgt cgccgcgatg
     3541 gacgagcacg cgcgcttcga cgcggacctg ctaccgctgt tgttcgagct cggcgtcatg
     3601 gcggtggaag tccccgcgga gctcgggggc gcagggtcga gctttttcaa cgccattttg
     3661 gcggtcgaag cgctcgcaga agtcgatccg agtatcgctg tgttggtcga cgttcaaaac
     3721 acgctcgtca acaacgcgct gcttcgctgg ggcaacgcgg agcagaaggc gcggtacttc
     3781 ccgaagctcg cgagcagctg ggtcggctcc tacgcgttga gcgaggcctc gagcggctcg
     3841 gacgctttcg ccctcaagac tcgcgccgag cgccgcggtg accgctggct tctccgcggg
     3901 cgcaaactgt ggatcacgaa cgcgaac
//
LOCUS       DASTKS010001317         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1554606, whole genome shotgun sequence.
ACCESSION   DASTKS010001317 DASTKS010000000
VERSION     DASTKS010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554606"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="VFQ35_28195"
     CDS             <1..1986
                     /locus_tag="VFQ35_28195"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET9934631.1"
                     /translation="GIPSHAGPQTPGSIHEGGELGYVLTHAFGAAFDHPELIVAAVVG
                     DGEAETGPLEGSWKGISFLNPVRDGAVLPILHLNGYKIAGPTVLARSSDEDITRLLSG
                     HGYEVHFVSGSEPGRVHAELGKVLGTCLERIASIQAAARKAGQVSERPRWPVLVMRTP
                     KGWTGPHEVDGKLVEGTFRAHQVPVSDVRSEASHLRLLEDWLKSYRPEELFDEQGRLS
                     PALAELSPRGQRRMSGNPLANAGQSNPTLSLPRVADYALEVSRPGRELHESTRQLGMM
                     LRDVYRDNPDSFRLMCPDETNSNRLGAVFEREKRCLVAPPRPGDENVAADGRVMEVLS
                     EHNCQGWLEGYTLTGRHGLFATYEAFALIVASMATQHAKWLEACAELAWRAPVPSLNY
                     LLTSTCWRNDHNGFSHQGPGFMDTILSKKGSVARVYLPPDANCLLAVAEHCLRSKDHV
                     NLIIIDKQPQLQWLDFASAQRHCEQGASVWEWASTKGAPDIVLACAGDTATQETLAAA
                     AWLREHAPSVCVRVVNVVDLMSLAMPDEHPHGMRSERFDDLFTRDVPVVFAFHGYAGA
                     VHTLLHGRSDARRFHVRGYREEGTTTTPFDMLVLNHTSRFHLAELALSHTRSKPPDVE
                     LLTRECHAALARHTEYVRECFEDLPEIKDFRWRGDSK"
     gene            1983..3077
                     /locus_tag="VFQ35_28200"
     CDS             1983..3077
                     /locus_tag="VFQ35_28200"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET9934632.1"
                     /translation="MTERVLTLNAGSSSLKASLYESAGSDLRELFDMTLESRRTSAHE
                     SIARVFEQLTAKHPAEIGTVAHRIVHGGAEFSRPVRVDDVVLARLEQLRPLAPLHLPP
                     GLEILRAALERLPGAAHVACFDTAFHERLPDVARRLALPEPLDAAGVRRYGFHGLSYE
                     YVLSSLGEPAPPRLIVAHLGSGSSLAAVRDGRSVDTSMSFTPSGGIPMGTRAGDLDPG
                     VLLYLLRERKLTAEQLEHVVDHESGLMGVGGSADMAQLIERTRSNDARARAAVELFAY
                     AIKKQIGAYVAALGGLDALVFTGGIGEHAAPVRALACDGLAYAGVELDPRLNAMNAPV
                     ISLSGARCSVRVVKTRESIVMARAALKLAH"
     gene            complement(3343..>3583)
                     /locus_tag="VFQ35_28205"
     CDS             complement(3343..>3583)
                     /locus_tag="VFQ35_28205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934633.1"
                     /translation="PRKHDLLIGAANLDPAIGESADIDMRVRGSRAGNGGFGYQAEPH
                     PVFHVLADGTLEIVFGSDWGSQNLADTYVMRLAPM"
BASE COUNT          610 a         1161 c         1173 g          639 t
ORIGIN      
        1 ggtataccga gccacgcggg accgcagact cccggctcga ttcacgaggg tggtgagctc
       61 ggttacgtgc tcacgcacgc gttcggcgca gctttcgacc atccggagct gatcgtcgcc
      121 gccgtcgtgg gcgacggcga agcggaaacg ggtccgctgg agggatcgtg gaaggggatt
      181 agcttcttga acccggtgcg ggacggcgcc gtgctgccga tattgcacct gaacggttac
      241 aagatcgcag ggcccaccgt gctcgcgcgc tcgagcgacg aagacatcac gcgcttgctc
      301 tccgggcacg gctacgaagt ccacttcgtc agcggaagcg agccgggtcg ggtacacgct
      361 gagcttggca aggtgctcgg cacctgcctc gagcgcatag caagcattca agctgcggct
      421 aggaaagccg gtcaagtgag tgagcgaccg cgctggcccg tgctcgtgat gcgcacgccc
      481 aagggatgga cggggccgca cgaggtcgac ggcaagctcg tggaggggac gttccgcgcg
      541 catcaagtcc cagtttcgga cgtgcgcagc gaagcctcac acttgcgctt gctcgaagat
      601 tggctgaaga gctaccggcc cgaagagctc ttcgacgaac aggggcgcct gtccccggcg
      661 ttggccgagc tttcgccgcg cggacagcgg cgcatgagcg gcaatccgct tgccaatgcg
      721 ggtcagtcga atcctactct gagcctgccg cgggtcgccg actacgcgct cgaggtgagc
      781 cgtcctggcc gcgagcttca cgaatcgacc cgccagctcg gcatgatgct gcgcgacgtg
      841 tatcgcgaca atccggactc gttccggttg atgtgccccg acgagaccaa ctcgaaccgg
      901 ctcggggcgg tattcgagag agaaaagcgc tgcttggtcg cgccgccgag accgggagac
      961 gagaacgtgg ccgcggacgg gcgcgtgatg gaagtcttga gcgagcacaa ctgccaggga
     1021 tggctcgaag gctacacgct gaccggtagg cacggcttgt tcgcgaccta cgaagctttt
     1081 gcgctgatcg tcgcatccat ggccacgcaa cacgccaagt ggctcgaagc gtgcgccgag
     1141 ctcgcctggc gcgcgccggt gccatcgctc aactacctgc tcacctcgac gtgttggcgg
     1201 aacgatcaca acgggttcag ccatcagggc ccgggcttca tggacacgat cttgtcgaag
     1261 aagggaagcg tcgcgcgcgt ttatctacca ccggacgcga actgcttgct cgcggtggcg
     1321 gagcattgcc tccgtagcaa agaccacgtc aacctgatca tcatcgacaa gcagccgcag
     1381 cttcaatggc tggatttcgc gtccgcgcag cgtcactgtg agcaaggcgc ttcggtttgg
     1441 gagtgggcaa gtacgaaggg agctccagat atcgtccttg cgtgcgcggg agacaccgcg
     1501 acccaggaga cgctcgccgc cgctgcgtgg cttcgagaac acgcgccgtc ggtctgcgtt
     1561 cgcgtcgtca acgtcgtgga tctgatgtct ctcgccatgc cggacgagca cccgcacggc
     1621 atgcgcagcg agcgtttcga cgatttgttc acgcgcgacg tcccggtcgt gttcgcgttt
     1681 cacggctacg ccggcgcggt tcacacgctg cttcacggtc gctcggacgc acgacgcttt
     1741 catgtgcgcg gctatcgcga agaagggacc accaccacgc ccttcgacat gttggtgctg
     1801 aaccacacca gtcgtttcca tctcgccgaa cttgcgctga gccacacccg gagcaagccg
     1861 ccggacgtcg agctactcac gcgggagtgc cacgccgccc tcgcgcggca caccgaatac
     1921 gttcgcgagt gtttcgaaga tctccccgaa atcaaggatt tccgttggcg tggtgattcg
     1981 aagtgaccga gcgcgttttg acgctgaacg caggctcgtc gagcctcaaa gcgtcgctgt
     2041 acgagagcgc tggctccgac ttgcgcgagc tcttcgacat gacgttggaa agcagacgca
     2101 cgagtgccca cgaatcgatc gcgcgcgtct tcgagcaact cacggcaaag catccggccg
     2161 agatcgggac cgtggcgcac cgtatcgtcc acggcggcgc ggagttctcg cgtcccgttc
     2221 gcgtcgatga cgtcgtgctc gcgcgcctcg aacaattgcg acctttggcg ccgcttcatc
     2281 tcccacccgg gttggaaatc ttgcgcgcgg cgctcgagag gttacccggc gccgcgcacg
     2341 ttgcctgctt cgatacggcg tttcatgagc gccttcccga cgtcgcgcgt cgactcgcgc
     2401 tgcccgagcc gctggatgcg gccggcgttc gccgttacgg ctttcatggg ttgtcgtacg
     2461 agtacgtgct gtcgagtctc ggtgaaccgg ctcctccgcg cctcatcgtg gctcaccttg
     2521 gaagcggctc gagcctcgcg gcagttcgag acggccgctc cgtcgacacg agcatgagct
     2581 ttacaccgag tggcggcatt ccgatgggca cgcgggccgg cgatctcgac ccgggcgtgc
     2641 tgctctacct cttgcgggag cgcaagctca cggccgagca gctcgagcac gtggtcgacc
     2701 acgaatctgg cttgatgggc gtcggtggca gcgccgatat ggctcagctc atcgagcgga
     2761 cccgctcgaa cgacgcccgc gcgcgtgcgg ctgtcgagct cttcgcgtac gcgatcaaga
     2821 agcagatcgg cgcgtacgtc gccgcgctcg gcggcctcga cgcgttggtc ttcactggtg
     2881 gcatcggtga gcacgccgcg ccggtgcgag ctctagcttg cgatggtctc gcgtacgccg
     2941 gcgtcgagct cgacccgcgg ctgaacgcga tgaacgcgcc ggtcatcagc ctgagcggcg
     3001 cgcgttgttc ggtgcgcgtc gtgaagacac gggagagcat cgtcatggcc cgcgctgcgt
     3061 tgaagctagc ccactgatgc ttctcctgaa cgccgggacc cggagtagtc gcagggccgg
     3121 tctttggaac cttgcgccag tcatcgaatg cttcccgcag tggcgaggca aggagcactt
     3181 cctcttttct tagcccccgc ggtggccgac gaatacgccg tgctcatcgc cggaccggcg
     3241 cggcgtgacg tcaacgcgcg cctcgccggt tgctaaccgt cgaaacgcga acgccccgag
     3301 aaaatcccga ggcgttctag acaccggcaa tccgcgctgg tcttacatcg gcgcgaggcg
     3361 catgacgtac gtgtcggcca gattctgaga gccccagtcg ctgccgaaca cgatctcgag
     3421 cgtgccatcg gcgagaacgt ggaacaccgg atgcggctcc gcttggtaac cgaagccgcc
     3481 gttgccggcg cggctgcccc ggacccgcat atcgatatcc gcgctctcgc cgattgctgg
     3541 gtccaggttc gcggcgccga tcagcagatc gtgttttcgc ggg
//
LOCUS       DASTKS010001318         8671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_2002440, whole genome shotgun sequence.
ACCESSION   DASTKS010001318 DASTKS010000000
VERSION     DASTKS010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002440"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFQ35_28210"
     CDS             complement(<1..1187)
                     /locus_tag="VFQ35_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9934634.1"
                     /translation="MDRLTTKSQEAVRAAIDTASRRGNPEVIPEHVLVAVLEQDGGVG
                     APLVERAGAKSRALIQDLNQRIEGLPRVSGGAEPSFGRRTTPFLNKAEDEAKKLKDDF
                     VSVEHFLLAAADGDHETKATFAKHGLTYDKLVKALAEIRGSQRVTDQDPEGKFQALEK
                     YTRDLTALAKRGKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIAEGIAH
                     RIASGDVPESLKDKRILALDLASMVAGSKFRGEFEDRLKAVLKEVEASAGGVILFIDE
                     LHTLVGAGAAEGAMDAANMLKPALARGELRAIGATTLDEYRKHIEKDAALERRFQPVM
                     VSEPSVEDTIAILRGLKERYEVHHGIHIQDAALVAAATLSHRYISDRFLPDKAIDLVD
                     EAA"
     gene            complement(1509..1874)
                     /locus_tag="VFQ35_28215"
     CDS             complement(1509..1874)
                     /locus_tag="VFQ35_28215"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9934635.1"
                     /translation="MATKGTKRIGQLIRSARMHRGLTQKDLARKLGVRSGSYVGMLES
                     GLCKGIGISRLEEIANYLDVDGVDLYRWMAAAGHLPPNLFGALMKRPERWDDIISLLG
                     PALAGARGPRSSRRPRARS"
     gene            3218..4816
                     /locus_tag="VFQ35_28220"
     CDS             3218..4816
                     /locus_tag="VFQ35_28220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934636.1"
                     /translation="MSTGGAGAGGSEAPGSGGSTSLASGGRASGGASSGSGGGGSGSP
                     PTNGGSCSKTDSPSMAACTVIESIGVFVSPKGSDDKGRGTRELPFKTLRTAITEARKQ
                     SKRVYACSTGGAFQETVTLDEASDQTELYGGFDCVDWTYAADAPTTVLSDTPRALVAS
                     ELTSLVIEDFSFVAANAVAPGESSAAAWIANSKSVFFRRVSLTAGNGADGADGAGTKE
                     PAPKGATGSSGVDACIAQHAPNEGGMEIESLCDGQPSGSIGGAGGDGGNDNKSALSGS
                     PGQPITNDRGLPGVGQTATPGWNCAVTGSSGGGGAQLGSDAMSQPAAAGGVSSATAAG
                     TLTADGFTGPAGQDGANGTPGQGGGGGGGAMAPGTCGGVLPRTGASGGGGGGGGCGGK
                     GGKGGGAGGGSFALVSFRSSINLEGGVLTAQRGGNGGKGGYGQPGGAGGLGGSGGKGV
                     DSSSACPGATGGKGGDGAHGGGGAGGPSAAIAVAGFQPVRQGAPLLKLSKDSSNGGDG
                     GAGTGKGPGVGSAGLVTEVLSFDR"
     gene            complement(4883..7399)
                     /gene="gyrB"
                     /locus_tag="VFQ35_28225"
     CDS             complement(4883..7399)
                     /gene="gyrB"
                     /locus_tag="VFQ35_28225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9934637.1"
                     /translation="MTSCRVVSSDGERTNEDSILTTEAQEPTPKASEYDSNSITVLEG
                     LEAVRKRPGMYIGDVHDGSALHHLVWEVVDNAVDEHLAGHCKVTKVVVHFDGSVTVED
                     DGRGIPVGMMADRGVSAAEVVMTVLHAGGKFDHASYKVSAGLHGVGVSAVNAVSEWLR
                     LEIHREGKVWFQEYRRGVPQGSIAAIGESDRTGTKITFKPDHEIFSNTEYNYDILANR
                     LRELAFLNSGLVIELKDERGDRSETYEYKGGICEFVALLSKTKEPVHEEVIAFNGEFP
                     AGEGKANIVVDCALQWTNSFAEHILCYTNNVFNKDGGTHLTGLRSALTRSLNNYGQQQ
                     NLFKDAKNGLTGDDTREGVICVIHVKAPDASFDSQTKSKLVSSEVRSIVENAVNDKLS
                     VFFEEHPQTARKILEKAVIAAKAREAARKAREVVRKGVLDNTSLSGKLADCQSKDPAI
                     SELYIVEGESAGGSAKQGRDRHFQAILPLKGKILNVERARLDRMLSSQEVATLISALG
                     CGIDDTGNFDLSKLRYHKIILMTDADVDGSHIRTLLLTFFYRQMREVIEKGYLFIAQP
                     PLYGVRKGKRLQYMKDQIALDRFLLENAIDDLTVQAQKGPSLSGMPLYNLATRLRSLR
                     GIFGKIDRRADARVVAALLRTSALTLADFRDAAKVEDAAKKLEAALTERYPDLLPLSV
                     TVDWEKAHGAGRIVVRFRPGAATRPAVLDWELAESAEYQELLSIWEDIRSIGPAPYTV
                     RVGSSEPLEISDPEALEAFIAERGRKGMQITRYKGLGEMNADQLWETTMSPDARTLLQ
                     VKVSDPVRADELFSVLMGDQVEPRRQFIEQNALNVRNLDI"
     gene            complement(7634..>8671)
                     /gene="recF"
                     /locus_tag="VFQ35_28230"
     CDS             complement(7634..>8671)
                     /gene="recF"
                     /locus_tag="VFQ35_28230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET9934638.1"
                     /translation="EPGPRLNVISGDNGQGKTSLLEALYFVATTRSFRTERLPTLLRH
                     GSEQGVVRAELAEAGRSREQVMVLSPTVRRATIDGKRPARLVEYAQRTPVVAFHPGDL
                     ELVAGAASFRRRLLDRVALFIDAAGYEHKSRYERALKERQRALEERGERAAEHEAFEQ
                     VAAQEGARYQLARERAAKTLVELVPARFERLAPSALSLDASYVPGGSTDVSVFRRELE
                     QRRTKDRFRRSSSFGPGRDEFELRLDGSPARSHASQGQQRILTLALKLAELDAISEAR
                     GAPAVLLLDDVSSELDPARTGAVYEVLRTSAGQVFVTTTRPELFPTPDRASERIDFGV
                     ERGTLRVSPAA"
BASE COUNT         1487 a         2729 c         2838 g         1617 t
ORIGIN      
        1 ctcgcggcct cgtccaccaa atcgatggcc ttgtccggca agaagcgatc gctgatgtag
       61 cgatggctca gggtcgcggc cgcgacgagc gccgcgtcct gaatgtgaat gccgtggtga
      121 acctcgtagc gctccttcaa gccacgcaag atcgcgatcg tgtcctccac gctcggctca
      181 ctcaccatca ccggctggaa gcgacgttcg agcgcggcgt ccttttcgat gtgcttgcgg
      241 tactcgtcga gcgtggtcgc gccgatcgcc cggagctcac cgcgcgcgag cgccggcttc
      301 agcatgttcg cagcgtccat cgcgccctct gcggcgcccg cgcccaccaa cgtgtgaagc
      361 tcgtcgatga agagaatcac gcccccggcg gacgcctcga cctctttcaa caccgccttc
      421 aggcgatctt cgaactctcc gcgaaacttc gagcccgcga ccatgctcgc caaatcgagc
      481 gccagaatgc gcttgtcctt caacgactcg ggcacgtcgc cgctcgcgat tcggtgcgcg
      541 atgccctctg cgatcgcggt cttgcccacg ccaggttcgc cgatgagcac cggattgttc
      601 ttcgtgcgcc gcgagagcac ctgcatcacg cgccggatct cctcgtcgcg cccaatcacc
      661 gggtcgatct tgccgcgctt cgcgagcgcg gtgaggtcgc gcgtgtactt ctcgagcgct
      721 tggaacttgc cctctggatc ttgatccgta acgcgttggc tgccgcgaat ttccgcgagc
      781 gcctttacga gcttgtcgta agtgagtccg tgcttcgcga acgtcgcctt ggtctcgtgg
      841 tcaccgtcgg cagccgcgag caagaagtgc tcgaccgaaa cgaagtcgtc cttgagcttc
      901 ttcgcttcgt cttcggcctt gttcaagaac ggcgtcgttc tgcgaccgaa gctcggttct
      961 gcgccgccag agacgcgcgg gagtccctcg attcgctggt tcaaatcctg gatcaacgcg
     1021 cgtgatttcg cgcccgctct ctcgaccagc ggcgcgccaa cgccaccgtc ctgctccaga
     1081 acggcgacca agacgtgctc cggaatgacc tccggatttc cgcgtcggga cgcggtgtct
     1141 atcgccgctc gcacggcttc ttggctcttg gttgtcagtc gatccatccg catgatgagg
     1201 ctcccacggc actttccgtg ccagaacgca acgtaagtcg ggtcttcgag caatcaagcc
     1261 ccgccgtgac accaaccatt cgtccgtgac gtcgagcagc cttcgccggt tcgtggatga
     1321 cggcgcaaaa ggcgccgaac gccgccgcag tctcgcggat cagccgaatt gaaatgggcg
     1381 tcgcgtcggg acgaatgtcg gaacgtgttc gtcgggctcg ccgaactgaa gcagggcgtc
     1441 gcgtgggaga aggtgccctc ctcccacccc aacgcaaggc caggcgcggc cgacgactat
     1501 cgcttcgctt agcttcgcgc gcgtggccgg cgcgaagagc gcggcccccg agctcccgcg
     1561 agcgcgggac ccaagagcga aatgatgtcg tcccaccgct cggggcgctt catcaacgct
     1621 ccgaacaagt tgggcggcaa gtgacccgcc gctgccatcc agcggtagag gtcgacgccg
     1681 tccacatcga gatagttcgc gatctcttcg agacgagaaa tgccgatccc cttgcacaag
     1741 ccagactcga gcatgccgac atagctgccc gagcgaactc ctaacttgcg tgcaaggtcc
     1801 ttttgagtga gaccgcgatg catgcgagcg gagcggatta gctgaccgat tcgtttggtg
     1861 cctttcgtag ccatggatcc cgttgccgca ctagccggtg acgccaagcg cggcccctcc
     1921 tttcgttcct agcgagcgga aagggtctat gggttggtaa acccgtagat cgccctctga
     1981 cgccgactac cttaactcca acttctggct gattggtagt ggcgaaaaac gtctcgggcc
     2041 tgattttcct gaggggtgat gaatcatcca gaagggttcc tcgttttctc tgctcgaagc
     2101 ggtgacccac gagttcagat gagttggagg cgcggaattc gcgctacggc tgacgcgcta
     2161 attttctccg tgtgtcacgt cgttgacgag gtttgccacc ctccgggtag agctccgcgt
     2221 cggcactgaa atcgcagtct gagctcattt tgacaccctc aagggtgctg ctgctgaaac
     2281 gagctgcaga gctgcacgat tcccgctcgc cggaagcttc ggcgtgagcg aacacgctca
     2341 tttcgcagct gtcactctgg cctaagggcc ggcgtgttca tgcctgcttt cggctcattc
     2401 gttccgccac tcagtgcgtt cgccaggtga cgaagccgta catttcgcaa gaatgccccg
     2461 agcgtttttg gcacacgctc gaccgatcgt tgcgaaattc gggagagcac cgaaagcccg
     2521 gactgaccgg cgagacacga tgcgacgaat ttcgaacgat cgagatcgga cgctgaccga
     2581 cgaacgtcgg tcgactttgc gtgagtgaac ctcgagagtt gtggattagc gcggcgaatc
     2641 gagcttcgaa ttgatgcttt ggaatcgaag tgcggagttg ggctcagcgc tgaacccgca
     2701 ctgatcgagg cctttgtctt cgcccacatc cacgagttct gtcgatcatc gactcgaagc
     2761 tcgcgcgcgc cgtgcctcgg cccgagcttc cgttcaaaag cggcaggcgc caccagtcgc
     2821 agttttctgc gtgattccga gcgggttgcg gcggatcgcg ctggtgggaa acggtgcgcc
     2881 cgagtagcga atgggcggac atggtcgtgt agatttatgg ctgagctgcg ctgagtaggg
     2941 tgcaaccacg cacctgctcg agtaataggg ttctcagatg ctttggtccc acttgcctcg
     3001 gtcatcgcgc gttgaagcaa acagcgaggc acgggggccg cgacgaggga gagcgagtcg
     3061 tcgcctcgtc ggcaacgcga gcgccctgat ttcggctgca tttgccgtgt gcgggtgcgg
     3121 ccaggagttc tcgaatggcg ccgactcgag cgggggcgca cctgcgttcg gcggagcgaa
     3181 cacggccgga gcgaacatgg ctgcgggcgg aaaagcgatg tcgaccggcg gcgcgggcgc
     3241 ggggggctcc gaggcgccgg gcagcggtgg aagcacctca ctggcgagcg gaggtcgggc
     3301 ctctggcgga gcaagctccg gtagtggcgg aggtggcagc ggctcgcccc ctacgaacgg
     3361 cgggagctgc agcaagaccg acagtccgtc gatggcggct tgtaccgtaa tcgagtcaat
     3421 cggcgtcttc gtgtcgccga agggaagcga cgataaaggt cgcgggaccc gcgagttgcc
     3481 gttcaagaca ttgcgcacgg caatcaccga agcgcgcaag cagagcaagc gcgtgtacgc
     3541 ttgttctacc ggaggcgcgt tccaagagac ggtcacgctc gatgaggcct cggatcaaac
     3601 cgagctttat ggcggcttcg attgcgtcga ctggacttat gctgccgacg cgccgacgac
     3661 cgtgttgagc gatactcccc gcgcgctcgt cgcttcggag ctgacgtcgc tcgtgatcga
     3721 agacttctct ttcgtcgcgg caaacgccgt cgccccggga gagagcagcg ccgcggcttg
     3781 gatcgcgaac tcgaagagcg tcttctttcg tcgtgtctca ctcacagccg gaaacggtgc
     3841 cgacggtgcc gacggcgccg gcaccaaaga gcccgctccg aagggcgcga caggctcgag
     3901 cggcgtcgac gcctgcattg cgcagcacgc tccgaacgaa ggcggcatgg agatcgagag
     3961 tctgtgcgac ggtcagccga gcggcagcat cggaggagct ggcggtgacg gcgggaacga
     4021 caataagagc gctctcagtg gctcgccggg tcagcccatc accaacgacc gagggctccc
     4081 cggcgtcgga cagaccgcaa cacccggttg gaattgcgcc gtcaccggtt cgagcggcgg
     4141 cggtggggcg cagctcggca gcgacgcgat gtcacaaccg gcggcggcgg ggggagtcag
     4201 cagcgcgacc gcggccggca ccctaaccgc ggatggcttc accggacctg cgggtcaaga
     4261 cggcgcgaac ggcacgccgg gccagggcgg tggtggcggc ggtggcgcca tggcacctgg
     4321 aacgtgcgga ggagtattgc cgcgcaccgg cgcgtccggc ggtggtggcg gaggcggtgg
     4381 ctgcggcggc aagggtggaa aaggcggagg cgccggtggc ggaagcttcg ctctcgtatc
     4441 gttccgctcc tcgatcaatc tcgagggcgg cgtgctgacc gctcagcgcg gcggcaacgg
     4501 aggcaaaggc ggttacggcc agcccggagg cgcgggcggt ctcggtggct ccggcggtaa
     4561 gggcgtcgat tcctcgagcg cttgtcctgg tgcaaccggc ggcaaaggcg gcgacggagc
     4621 gcatggtggc gggggcgctg gcggaccctc tgcagcgatc gccgtcgcgg gatttcagcc
     4681 cgttcgacag ggtgcgccgc tcttgaagct gtcgaaagat tcgtcgaacg ggggagacgg
     4741 cggcgccggc actggcaaag gcccaggcgt tggcagtgcc gggctcgtca ccgaagtatt
     4801 gtcgttcgat cgttgagccc tcgcgagcct caccggctcg ctgtgcaggg acgcaaacga
     4861 gccggcgttc gacggagtcc gctcagatat cgaggttgcg cacgttcagc gcgttttgct
     4921 cgatgaattg ccgccggggc tcgacttggt cgcccatgag cacgctgaag agctcgtcgg
     4981 cacgcaccgg atcgctcacc ttcacctgga gcaaggtgcg cgcgtccggg ctcatcgtgg
     5041 tctcccacag ctggtcggca ttcatttcac cgagaccttt gtaacgggtg atctgcatgc
     5101 ccttgcgacc gcgctcggcg atgaaggctt cgagagcctc cggatccgag atctcgagtg
     5161 gctcactcga gccgacgcgc acggtgtacg gtgcaggacc aatcgagcgg atatcctccc
     5221 aaatcgagag cagctcttgg tactcggcag actccgcgag ctcccaatcg agaacggccg
     5281 ggcgcgtcgc ggcgccggga cggaaccgca cgacgatgcg accggcgccg tgcgccttct
     5341 cccagtcgac cgtaacgctg agcggcaaca agtccgggta gcgctcggtc aaagccgctt
     5401 cgagcttctt cgccgcgtct tcgaccttgg ccgcatcgcg aaaatcagcg agcgtgagag
     5461 cgctcgtacg gagcagcgcc gccacgacgc gcgcgtcggc acgccggtcg attttgccga
     5521 agatgccgcg cagcgaacgg agccgcgtcg ccaagttgta gagcggcatg cccgagagcg
     5581 atggcccctt ctgcgcttgc accgtcaaat cgtcgatcgc gttctccaag aggaaccgat
     5641 cgagcgcgat ctgatccttc atgtactgga gccgcttgcc cttacgcacg ccgtagagcg
     5701 gcggctgcgc gatgaacaag tagcccttct cgatgacctc gcgcatctgt cggtagaaga
     5761 acgtgagcag cagcgtgcgg atgtgactgc cgtcgacgtc ggcgtcggtc atcaggatga
     5821 tcttgtgata gcgaagcttc gaaagatcga agttgccggt atcgtcgatt ccgcagccga
     5881 gcgcggaaat gagcgtcgcc acctcttgag aagagagcat gcggtcgagg cgtgctcgtt
     5941 cgacgttgag gatcttgcct tttagcggca aaattgcctg aaaatggcga tctcgaccct
     6001 gcttcgcgct gccgccggcg ctctcaccct cgacgatgta gagctcgctg atggccggat
     6061 ctttgctctg acagtcggcg agcttgccgg agagtgacgt gttgtcgagg actcccttgc
     6121 gcacgacttc gcgagccttg cgggccgcct cgcgcgcctt cgccgcaatg accgccttct
     6181 ccaagatctt gcgagccgtc tgggggtgct cttcgaagaa gacggagagc ttgtcgttca
     6241 ccgcgttctc gacgatggag cgcacctcgc tcgagacgag cttcgacttc gtctgcgaat
     6301 cgaaggaggc gtcgggcgcc ttcacgtgaa tgacgcagat tacgccttcg cgcgtatcgt
     6361 cgccggtgag cccgttcttc gcgtctttga agaggttttg ttgctgaccg tagttgttca
     6421 gtgaacgcgt cagcgccgaa cgcaagccag tgaggtgcgt cccaccgtct ttgttgaaga
     6481 cgttgttcgt gtaacagagg atgtgctcgg cgaagctgtt ggtccactgc aacgcgcagt
     6541 cgacgactat gttcgccttg ccttcgcccg ctggaaactc gccattgaag gcgatgacct
     6601 cttcgtgcac cggctctttc gtcttgctga gcagcgcaac gaactcacag atgccgcctt
     6661 tgtattcgta agtctcgctg cgatcgccgc gctcgtcttt gagctcgatg accaaccccg
     6721 aattcaaaaa ggcgagctcg cgcaggcggt tcgcgaggat gtcgtagttg tattcggtgt
     6781 tggagaagat ctcgtggtcg ggcttgaagg tgatcttggt cccggtgcga tcgctttcac
     6841 cgatggcggc gatcgagcct tgcggaactc cgcggcggta ctcctggaac cagaccttgc
     6901 cttcccgatg gatctcgagc cgcagccact ccgaaacggc gttcacggcg ctcacgccca
     6961 cgccgtgcaa gccggcgctg actttgtagc tcgcgtggtc gaacttaccg ccggcatgca
     7021 gcaccgtcat gacgacctcg gccgcgctga cgccccggtc cgccatcatt ccgaccggaa
     7081 tgccgcgccc gtcgtcttcc accgtgaccg agccgtcgaa gtggacgacc accttggtga
     7141 ccttgcaatg ccctgccagg tgctcgtcga cggcgttgtc gaccacctcc caaacgaggt
     7201 gatgcaacgc cgaaccgtcg tgcacgtccc cgatgtacat gccggggcgt ttacgtaccg
     7261 cctccagccc ttcgagaacc gtgatgctgt tggagtcgta ttccgacgct ttgggcgtcg
     7321 gttcttgagc ctctgtcgtc aaaatcgaat cctcgttggt gcgctcgccg tcgctcgata
     7381 cgactcggca gctggtcact gcgggtcgac gccgaaacgg tgctgtagcg cctctcgaag
     7441 ctggctccac gagcccgcaa aaccggcttt tcttatagta ttttccgagt cgaaccgcaa
     7501 ctcaattagc ctgaatttcc aacgaaaaac gccccctcgg ggctgccatt ttcggtgttc
     7561 gccgtacccc tcgaaagcga cgcacgtaac ctcgttttcc ggtgtccgta ggaccggtcg
     7621 aacacggcgt ggatcaagcc gctggcgaga cgcgcaaggt gccgcgctcg acgccgaaat
     7681 cgatgcgctc actcgctcgg tcgggggtcg gaaatagctc ggggcgcgtg gtggttacga
     7741 agacttggcc ggcgctcgtg cgcaggactt cgtaaaccgc gccggtccgc gccggatcga
     7801 gctcgctcga gacgtcgtcg agcaagagga ccgcgggggc gccgcgcgcc tccgaaatcg
     7861 cgtcgagctc ggcgagcttg agagccaaag taaggattct ttgctgcccc tgggaggcgt
     7921 gggagcgcgc gggactgccg tccagacgga gctcgaactc gtcccgcccc ggaccaaagc
     7981 tcgaggagcg gcggaagcgg tctttggtgc ggcgctgctc gagctcgcgg cggaagacgg
     8041 acacgtccgt cgagccgccc ggcacgtacg aagcgtcgag cgaaagcgcg ctcggagcga
     8101 gacgctcgaa gcgcgcgggc acgagctcga cgagcgtctt cgcggcgcgc tcgcgagcga
     8161 gctggtagcg cgccccttct tgagccgcga cttgctcgaa ggcctcgtgc tccgcggcgc
     8221 gctctccccg ctcttctagg gcgcgctggc gctctttcaa agcgcgctcg tagcggctct
     8281 tgtgctcgta gcccgcggcg tcgatgaaca aggcgacgcg gtccaagagg cgccggcgga
     8341 agctcgcggc gccggcgacg agctcgagat cgccggggtg gaaggcgacg actggcgtgc
     8401 gctgcgcgta ctcgacgagc cgcgccggtc gcttgccgtc gatggtcgcg cggcgaaccg
     8461 tcggcgagag caccatcacc tgctctcggg agcgaccggc ttcggcgagc tcggcgcgca
     8521 cgacgccttg ctccgagccg tgacggagca gtgtgggaag gcgctcggtg cggaagctgc
     8581 gcgtcgtggc tacgaagtag agcgcctcga gcaggctggt cttcccctga ccattgtctc
     8641 cggagatgac gttcagccgg gggccgggtt c
//
LOCUS       DASTKS010001319         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1555232, whole genome shotgun sequence.
ACCESSION   DASTKS010001319 DASTKS010000000
VERSION     DASTKS010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555232"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFQ35_28235"
     CDS             <1..1128
                     /locus_tag="VFQ35_28235"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9934639.1"
                     /translation="VTDAHGLDEAVRAELARLDERPGPDTGEALLDEAMLRTRRSFEE
                     RHLRELGIGCEACHGGAREHVEAPLAKKPSLAPESPYFRVTRADGSPLTPALEVNRAC
                     AKCHTVLFSRYPYTWEGGRRRNNPGGSTINSGEARDFLLGGCREQLSCVACHDPHRED
                     KKADLEALAGPRGDAICTRCHTGLAGSDAVRAHTHHAPETAGSRCINCHMPRKNMGLA
                     YELTRYHRIGSPNDPERVERDRPLECALCHADRSVDQIVLTMERWWSKRFDRNALARL
                     YGADLRINALRATLMAGLPHEQATASALAAQHNRTDLLPLVLEVMKNDYPLVRYYGKH
                     AARRLGADVDALDPAFAGGKVQDPANRRTVSHLVGEAEKSD"
     gene            1219..1917
                     /locus_tag="VFQ35_28240"
     CDS             1219..1917
                     /locus_tag="VFQ35_28240"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9934640.1"
                     /translation="MLIKRPQTASARVEATFGFVDLAGFTALTEAQGDEDAADVATRF
                     AEMTRSTLGPGDRLVKTIGDAVLVTCPDPESGVALVERLLVEAASEPRFPALRAGLHH
                     GEAVQREGDVFGAAVNLAARVASEAHAGEVLGTAPIADAARKSGIPVAELGIVPLKNV
                     RGAIPLFSLALVVGTNDTPVDPVCKTPVERRGAAGRLRYRGGEFWFCSLTCAAAFASN
                     PGWHAIESLRPRSR"
     gene            complement(1909..>2768)
                     /locus_tag="VFQ35_28245"
     CDS             complement(1909..>2768)
                     /locus_tag="VFQ35_28245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934641.1"
                     /translation="AGLDDDVRVELVTALAVLGNEAPGELWAGTMPAGLFRSRDRGQS
                     WEFNEALWQRPERPEFRASGWGGSALHSLLVDPRDALKLLVGISRGGVFSTEDGGKSF
                     ECQGLGLPSSEGASLPNPHRLARCRSNPDVVWCQHKRGVFRSVDGGHTFVVAPGASEL
                     FGFALAAHPHDGETAWFVPSHPPSQPGPFERALSVCKTRDGGATVQRKIRGLPQHFAY
                     DVVYRHALEVDDSGTLLAMGSSTGNLWMSDDGAESFQVVSHNLPPIHALCFARARLRS
                     SILPAPHST"
BASE COUNT          477 a          943 c          927 g          421 t
ORIGIN      
        1 gtgacggacg cgcacggcct cgacgaagcg gtgcgcgccg agctcgcgcg gctcgacgag
       61 cggcccggac ccgacacggg agaggcgctg ctcgacgaag cgatgctgcg gactcgccgg
      121 agcttcgagg aacgccactt acgcgagctc ggcatcggct gcgaggcgtg tcacggcggc
      181 gcgcgcgagc acgtcgaggc accgctcgcc aagaagccgt cgcttgcgcc ggagagcccg
      241 tactttcgtg tcacgcgcgc ggacggctca ccgctcacgc ctgcgctcga agtgaatcgc
      301 gcctgcgcca agtgccacac cgtgcttttt tctcggtacc cttacacttg ggaaggcgga
      361 cggcgccgca acaatcccgg cggtagcacc atcaactcgg gggaggcgcg cgactttttg
      421 ctcgggggct gtcgagagca gctctcctgc gtcgcttgtc acgatccgca tcgtgaagac
      481 aaaaaggccg acctcgaagc gctcgcgggg ccacgcggag acgccatctg cacgcgctgc
      541 catacggggc tcgccggcag cgacgcggtg cgagcccaca cccaccatgc tcccgaaacc
      601 gcgggcagcc gttgtatcaa ctgccacatg ccgcgaaaga acatggggct cgcgtacgag
      661 ctcacgcgct atcaccgcat cggttcgccg aacgatcccg agcgcgtcga gcgcgatcgt
      721 ccgctcgagt gcgcgctctg ccacgccgat cgcagcgtgg accagatcgt tttgacgatg
      781 gaacgctggt ggagcaagcg gttcgaccga aacgcgctcg cgcgcttgta cggcgcggat
      841 ttgcgcatca acgccctccg cgcgacgctc atggcggggc tcccccacga gcaggcgacg
      901 gcgtcggccc tcgcggcaca gcacaatcgg actgatcttt tgccgctcgt gctcgaggtg
      961 atgaagaacg actatcctct cgtgcggtac tacggaaaac acgccgcgcg gcggctcggg
     1021 gcagacgtgg acgcgctcga tcccgccttt gcgggcggca aagtgcagga tccggcgaac
     1081 cgacggacgg tctcccacct cgtcggggaa gctgaaaaaa gcgactaggt ctcccgccgt
     1141 tttcccacat tggagccgcg agccagccga ccatgcgtgc gcgctcggac gagaaaacac
     1201 gataagcttc cgggtagcgt gttgatcaag cgcccgcaaa cggcctcggc tcgcgtcgaa
     1261 gccaccttcg gcttcgtgga tctcgcaggg tttacggcgc tcaccgaagc gcagggcgac
     1321 gaagacgccg ccgacgtggc gacccgcttc gccgaaatga cgcgctcgac gctcggcccc
     1381 ggcgaccggc tggtgaagac gatcggagac gccgtgctcg tcacctgccc cgatccggaa
     1441 tcgggcgtcg cgctggtcga acggttgctg gtagaagcgg cgtcggagcc gcgcttcccc
     1501 gccctgcgtg ccggcctaca ccacggcgaa gcggtgcagc gcgaaggcga cgtcttcgga
     1561 gccgcggtca acctggcggc gcgggtcgca agcgaagctc acgcgggcga agtgctcgga
     1621 acggctccca tcgccgatgc ggcgcgcaag agcgggatcc cggttgccga gctcggcatc
     1681 gttccgctca agaacgtgcg cggcgcgatt cccctgttct cgcttgcgct cgtggtgggt
     1741 accaacgata cgcccgtgga tccggtttgc aaaacaccgg tcgagcgccg cggcgcggca
     1801 ggtcggttgc gctaccgggg cggcgaattc tggttttgct cgctcacgtg cgcggctgcc
     1861 tttgccagta atcctggttg gcacgcgatc gagagcctcc gcccgcgctc acgttgagtg
     1921 cggcgccggc aagatcgacg agcgtagccg cgcgcgagcg aagcacaacg cgtgaatggg
     1981 aggcaggttg tgggacacca cctgaaagga ctccgctccg tcgtcgctca tccagaggtt
     2041 gccggtgctg ctccccatcg cgagcagcgt tccactgtcg tcgacctcga gcgcgtgtcg
     2101 gtagacgacg tcgtacgcaa aatgctgcgg gagtccccgg attttgcgct gcacggtcgc
     2161 gccgccgtcg cgcgtcttgc agaccgaaag agctcgctcg aagggccctg gctggcttgg
     2221 agggtgggac gggacgaacc aggccgtctc gccgtcgtga ggatgtgccg cgagcgcaaa
     2281 cccgaacagc tcgctcgcgc ccggagccac gacgaaggtg tgcccgccgt cgacggagcg
     2341 gaacacgcct cgtttgtgct gacaccagac gacgtcgggg ttcgagcggc atcgcgccag
     2401 gcggtgggga ttcggcaacg aagcgccctc gctcgaaggc aaaccgagcc cctgacattc
     2461 gaacgacttg ccaccatcct ccgtggagaa gactccgcct cgcgaaatgc cgacgagcaa
     2521 cttgagcgcg tcgcgcggat cgaccagcag cgagtgcaac gccgagccac cccagccgct
     2581 cgcgcgaaac tcaggtcgct ccggccgttg ccacaaggct tcgttgaatt cccagctctg
     2641 gccacgatcg cgtgagcgga acagccctgc cggcatcgtc ccggcccaga gctcgccggg
     2701 ggcttcgttt cccagtaccg cgagagcggt gacgagctcg acccgcacgt cgtcatcgag
     2761 cccggccg
//
LOCUS       DASTKS010001320         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1555455, whole genome shotgun sequence.
ACCESSION   DASTKS010001320 DASTKS010000000
VERSION     DASTKS010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555455"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="hisH"
                     /locus_tag="VFQ35_28250"
     CDS             complement(<1..533)
                     /gene="hisH"
                     /locus_tag="VFQ35_28250"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET9934642.1"
                     /translation="MSERIVIVDVGLGNLRSVAKAVETAGAGASVELVRSSDPSVVRS
                     ADRLVVPGQGEFGNLADRLEGGLGDAIRERLEKKTPYLGICLGLQILFETSEEAPGKR
                     GLGWFAGSVKRLVGGPSIKIPHMGWNQLEPQNGGHPLLPAGEWFYFVHSYHAVPDDPT
                     VVRATCGYGDNRVTAAVG"
     gene            complement(530..1126)
                     /gene="hisB"
                     /locus_tag="VFQ35_28255"
     CDS             complement(530..1126)
                     /gene="hisB"
                     /locus_tag="VFQ35_28255"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET9934643.1"
                     /translation="MTHRKASIERATRETKISVEVDLDGTGQSQIETPLPFLSHMLDQ
                     IARHGSIDLKVKAEGDVEIDGHHTTEDVGIVLGNAVLQALGDRKGIRRYGSATLPMDE
                     ALVTAAIDLSGRAFFVFRVPLPKAKIGNFDVELAEVFFEGFARSAQANLHLRLHEGTN
                     LHHIVEISFKAFARALREAVEFDPRSPGIPSTKGSLLG"
     gene            complement(1123..1785)
                     /locus_tag="VFQ35_28260"
     CDS             complement(1123..1785)
                     /locus_tag="VFQ35_28260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934644.1"
                     /translation="MLLRSFLALSLVVAGCAHSGASGEPDRTLQAYSRALEAGRAQEA
                     YALLSSDAQKSIPFEAFQRMLKENPEEIKELARSLRRPAQPPQVTATVTPPNGPTLLL
                     VYEDGRWRVDASSIDLYSQATPERAVAGFVRAYENRRFDVLMRFVPDAQKKDLTPASL
                     EKAWTSDQKDDVEHMMQALRAALPTARFEVLGERATLAFGASGMLELVREHGTWKVED
                     LK"
     gene            1952..>3429
                     /locus_tag="VFQ35_28265"
     CDS             1952..>3429
                     /locus_tag="VFQ35_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9934645.1"
                     /translation="MRALSGPRPERIAILPLRNSVLFPMSVVPINVGRPRSVRLVEEL
                     LGQESALVGVLTQKNAETVEPAFDDLYTVGTLARVVKVIRLGPSNYSVVLNGLGRFSL
                     LEQQGLEPFMRADIERVPEASQSGAELVRLGQKLRESTREVLALLPNLPKETASILDN
                     VREPGALADLIASNFPEEHASIHVRQRILEAFDVKSRVELVLGMVTRQLEVLKVRDEI
                     SSQVQNELSRSQRDYVLRQQMRTIREELGETAEDDEVEQLRERIARAELPLEAERAAR
                     KQLSRLSSMQQQSAEYQVTRNYVEWLADLPWHRTSPDRIDVKEVRRCLDEDHYGLETV
                     KRRIVEYCAIRQLRKDKRGPILLFVGPPGVGKTSLGRSIARAMGRRYARISLGGVRDE
                     AEIRGHRRTYVGALPGRILKAMKEAGTVNPVFMLDEIDKLGADFRGDPSSALLEVLDP
                     EQNNTFADHYLDVPYDLSRVMFITTANLLDPIPAALRDRMEI"
BASE COUNT          578 a         1117 c         1118 g          616 t
ORIGIN      
        1 cccacggccg cggtcacgcg gttgtccccg tagccgcacg tcgcgcgcac caccgtcggg
       61 tcgtcgggca ccgcgtgata cgagtgcacg aagtagaacc actcaccagc ggggagcaga
      121 ggatgccctc cgttctgcgg ctcgagctgg ttccatccca tgtgcgggat cttgatgctg
      181 gggcccccga cgagacgttt gacgctgccc gcgaaccagc cgagcccgcg cttgcctggc
      241 gcctcttcgc tggtctcgaa gaggatctgc aggccaaggc aaattccgag gtacggcgtc
      301 ttcttttcga ggcgctcgcg gatcgcgtcg ccgagcccac cctcgagccg gtcggcgagg
      361 ttgccgaatt cgccctggcc cggcacgacc agccgatcgg cgctccgcac gacgcttgga
      421 tccgatgagc gcacgagctc gactgaagcg cccgcgcccg ctgtttcgac cgccttggcg
      481 accgagcgga gattaccgag tccgacgtct acgatgacga tgcgctcgct catccgagta
      541 acgatccttt cgtcgatggg atgcccggag agcgtgggtc gaattcgacc gcctcgcgca
      601 gcgctcgggc aaaagccttg aaggagattt cgacgatgtg gtggaggttc gtcccttcgt
      661 gcaagcgcaa atgaaggttc gcctgagccg agcgcgcgaa gccctcgaag aacacctccg
      721 cgagctcgac gtcgaaattg ccgatttttg ccttgggcag cgggacccga aagacgaaga
      781 acgcgcgccc cgagaggtcg atggcggcag tgacgagcgc ctcgtccatg ggtagagtcg
      841 ccgagccgta gcgacgaatg ccctttcggt cgccgagtgc ctgaagcacg gcgttcccca
      901 gcacgatgcc gacgtcctcg gtcgtatggt gcccgtcgat ctcgacgtcg ccctcggcct
      961 tgaccttgag atcgatcgag ccgtgccgcg cgatctggtc gagcatgtgg ctcaggaagg
     1021 gcaggggggt ctcgatctga ctctgaccgg tgccgtccag gtcgacctcg acggagattt
     1081 tggtctcgcg ggtcgccctt tcgatgctgg ctttgcgatg cgtcacttga ggtcttccac
     1141 tttccaagta ccgtgctcgc gcacgagctc gagcattccg ctcgctccga aggccagggt
     1201 cgctcgctcc ccgagcacct cgaagcgcgc ggtcggcaat gcagcacgca gggcctgcat
     1261 catgtgctcc acgtcgtcct tttggtcgct cgtccaagcc ttctcgagcg acgcgggggt
     1321 gaggtccttt ttctgagcgt ccggaacgaa gcgcatgagc acgtcgaagc ggcgattttc
     1381 gtacgcgcgc acgaagccgg caaccgcgcg ttcgggcgtg gcctggctgt agaggtcgat
     1441 gctggaggca tcgacgcgcc atcgtccgtc ttcatacacg aggaggagcg tcggcccatt
     1501 tgggggcgtc actgtcgcgg tcacctgggg cggctgcgcc ggtcggcgga gcgagcgtgc
     1561 gagctctttg atttcctcag gattttcctt cagcatgcgc tgaaaagcct cgaaaggaat
     1621 agacttttgc gcgtcactgg aaaggagcgc gtacgcctct tgcgcgcggc ctgcttcgag
     1681 cgcccgtgag taggcctgaa gggtgcggtc gggctcgccc gaggccccgg agtgggcgca
     1741 cccggccacc accaggctga gcgcgaggaa ggagcgcagc aacatcggcg caccgactta
     1801 ccacaggggc cgagaacgag cgactttgcc ctgcttcggc gatgcgggtg cgtaggcgtc
     1861 gctaatcggt cggcctttcc gcaggccttt ttcgcgaggc gagcatcgtc tccgctctgg
     1921 acgcggcgcg gcgtcgtact aatctgaccc catgcgcgcc ttatccgggc cccgccccga
     1981 gcgcattgcg attctcccgc tgcgtaactc ggtgcttttc cccatgtcgg tggtgccgat
     2041 caacgtgggg cggccccgca gtgtgcgtct ggttgaagag ctgctcgggc aagagagcgc
     2101 cctcgtgggc gttctcacac agaagaacgc cgagactgta gagccggcgt tcgatgacct
     2161 ctacacagtg ggcacgctcg cccgggtcgt gaaggtcatt cgccttggcc cttccaatta
     2221 cagcgtcgtg ctgaacggcc tcggccgctt cagcctgctc gaacagcagg ggctcgagcc
     2281 cttcatgcga gcggatatcg agcgagttcc ggaggcctcc cagtccggcg cggagctcgt
     2341 ccgcctcggg cagaagctcc gtgagagcac gcgggaggtg ctcgcgctgc tcccgaatct
     2401 gccgaaggag acggcgagca tcctcgacaa cgtgcgggag ccgggtgccc tcgccgatct
     2461 catcgcctcg aactttcccg aggagcacgc cagcatccac gtccgccaac gcatcctcga
     2521 ggcgttcgac gtcaagtcgc gcgtcgagct cgtgctcggg atggtcacgc ggcagctcga
     2581 ggtcctgaag gtgcgcgacg agatttcgtc gcaggtccaa aacgagctca gccggtccca
     2641 gcgagactac gtgctgcggc aacaaatgcg caccatccgc gaagagctcg gtgagaccgc
     2701 ggaggacgac gaggtcgagc agctgcgcga gcgcatcgcg cgcgccgagc tccccctcga
     2761 ggcagagcgc gcggcgcgca agcagctctc ccgcctgagc tcgatgcagc agcagtctgc
     2821 cgagtaccag gtgacgcgca actacgtcga gtggctcgcc gatttgccgt ggcatcgcac
     2881 gtcgcctgat cgcatcgacg tgaaggaggt ccgccgctgc ctcgacgagg atcactacgg
     2941 cctcgaaacg gtgaagcgcc gcatcgtcga gtactgcgcg atccgccagc ttcgcaaaga
     3001 caagcgcggc ccaatcttgc tcttcgtcgg gcctcctggc gtcggcaaaa cctcgctcgg
     3061 tcggtcgatc gcgcgggcga tgggccgccg ctacgcgcgc atctcgctgg gcggcgtgcg
     3121 cgacgaggcc gaaattcgcg ggcatcgccg cacctacgtc ggcgcgctgc cgggccgcat
     3181 cctcaaagcg atgaaggaag ctggaacggt caatccggtc ttcatgctgg atgaaattga
     3241 taagctcggc gcagacttcc ggggcgaccc gtcgtcggcg ctgctggaag tgctcgatcc
     3301 ggagcaaaac aacacgtttg ccgatcatta tctcgacgtg ccgtacgatc tttccagagt
     3361 tatgttcatc accacggcca atctgctcga tcccattccg gcagcgttgc gcgaccgcat
     3421 ggagattat
//
LOCUS       DASTKS010001321         8706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_214614, whole genome shotgun sequence.
ACCESSION   DASTKS010001321 DASTKS010000000
VERSION     DASTKS010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214614"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFQ35_28270"
     CDS             <1..600
                     /locus_tag="VFQ35_28270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934646.1"
                     /translation="QRFGYGTYRTRVAFGDCASNEEATQAVLGYFSDGADHNGNGIRD
                     ETEIDFQVLCGAPSLVYLTVFTDYEERPSGTVFRKLSHVIDFATGRTYDTPSPNEDAF
                     AAGPIDPTLVLELFSPGRFYEVGFEWHAAQIRFFIDAGAGEKTLWTLTDPARIPTGSV
                     QLVYNAWHPESHWFPLSSPADYPADDVVMSIDWFRFYAE"
     gene            complement(613..1959)
                     /locus_tag="VFQ35_28275"
     CDS             complement(613..1959)
                     /locus_tag="VFQ35_28275"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9934647.1"
                     /translation="MTTNTSIGSAFVITSTAFALGLAMQACSSNEGSGGGSGGFGFGG
                     AKQSSSSGGAREQGSGGMTASQGGGAGTSNTEGGRQSSGGMSAQSGGKASSGGSGVGG
                     SGAGGTDASGGMLGAGGRMGSGGRTGTGGRAGTGGDTATGGRQASGGALGLGGSGGST
                     QASGGSGGRNLLVPESGALLGLYYGDASIAATATKLGRSLPLHLTYYAWEDDFTRAET
                     AKDLSAGRIPFVNWELFGGELDDIIAGKYDSMLQQRARDTKALGKPLFIDFGAEMNGD
                     WSPWGGAQNGESAAKYLTVYRKVHDAFEDAGATNAIWAFCPNVTDEPRAAWNEALDYY
                     PGDDYVDWMCVDGYNWGDTDGGGWQSFEQVFQAIYPKLAAKNKPIMIGEMASAESGGN
                     KGAWIDAMIPTLKNKYPQIRGVLWFDVDKETDWRVSSSPAAEAAFKKMANDPYFNP"
     gene            complement(2078..3925)
                     /locus_tag="VFQ35_28280"
     CDS             complement(2078..3925)
                     /locus_tag="VFQ35_28280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934648.1"
                     /translation="MALGETVRAEQIRMLFGQNRAVLWANVAVSALVAGPLWILTSTP
                     RPLLAAWVALTIAVAAGRVALGRRFDTTRPQPKELEVWGQRFVAGSIAAGALWGVATL
                     VFFDATHAMTEALFSFAIGGMTAAAAGTWSSHLPTFRGYCLASLGPFIVRTFAVGDWP
                     HTAMGLMLLAYGIFIDRVARTNHASISKAFHLAFENADLLERLSRSQEVLENANRVLE
                     QRVRERTAEIEKQEAALRDAQRMESLGRLAGGIAHDFNNLLTIGMTNVDVLKDTQHLD
                     AVGNAALLETLDSMRRGAELVQRLLAFSSRLHLESKPVDLNEVIVDIRPLLVRLAGAR
                     VNVTFDLSPRPLRVLADVSRLRHVLINLVSNAGEAMANGGELRIVTQRTDALLGEPAE
                     LRPCALLRVDDTGIGMDEDTLRHAFDPFFSTKVGSPDHGLGLAAVHGIIQQSGGRVNA
                     ESKLGHGSRFFVHLPINEAVEHSPAPLIKGTASESARVATILIAEDEPMVRSTMRRNL
                     ARLGHRILEAEDGARALALARSHGNRIDLLITDVVMPNLGGAELVKTLAPEQRGMAVL
                     FISGYSWGEELPPSDERKGVAYLAKPFDTKSLDAKVTELLSIAGRLAAS"
     gene            4557..4964
                     /locus_tag="VFQ35_28285"
     CDS             4557..4964
                     /locus_tag="VFQ35_28285"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9934649.1"
                     /translation="MPKTSLARAEPLAFAAMRIVSGVLFLFHGLQKLINWPPGGHPVA
                     LASQVGVGGVIELVCGTLIALGWRTRGAAFIASGMMAVAYFQFHWKLAIANVAWAPVV
                     NRGELAVIYCFLFLFIATHGSGIASVDKLLARR"
     gene            5170..5595
                     /locus_tag="VFQ35_28290"
     CDS             5170..5595
                     /locus_tag="VFQ35_28290"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9934650.1"
                     /translation="MSTKRHFETAIELAANDVARDVSPASASAATKRQSFPRIHIQEE
                     DEHVVVLAVVPGFTREDLRIDVQRDVLALSGRVRCQVPQGFTALQKGRTHEDFSRHIK
                     LMTDVSRGETEARLERGVLTVRMRKHTAALRTPIPVRVP"
     gene            5608..6171
                     /locus_tag="VFQ35_28295"
     CDS             5608..6171
                     /locus_tag="VFQ35_28295"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HET9934651.1"
                     /translation="MRAALERTQEPSNLPHRPSAAPHASVAPDTSQVDDSWYPDAPRY
                     SMILDARARATNAPPTARAATLLPRPVNSLSTPPPPTTEKPRELAFDVYESPHAWLIL
                     VDAPGVDPDWISLSLGSRALHLEASVPTWTKQAGLKPGRYEMTVEVPAWVEADAIDAA
                     VENGLMRIRIAATKSGPRTIAILGEES"
     gene            complement(6232..6606)
                     /locus_tag="VFQ35_28300"
     CDS             complement(6232..6606)
                     /locus_tag="VFQ35_28300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934652.1"
                     /translation="MRFVEFMLEGGWSMWFVLAFGLVTLAMSVSFLRQPNVRQADIVR
                     SFSRATVFSIITGVSVNIAAVGAKVPANPEWANSPQIHLIVMQGIAEAIAPSSLGFTL
                     LALAWLVVAVGQRKLGREQMTS"
     gene            6676..7278
                     /locus_tag="VFQ35_28305"
     CDS             6676..7278
                     /locus_tag="VFQ35_28305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9934653.1"
                     /translation="MTNSRHAESIRASIVAVELAEPELLRALGRGDPNAFDQVYERLR
                     APLHSFLVRLSTDRELAEDLLQETWLRLARHAASLPHDTNLRAWLFTVARNLYRSHRR
                     WSLLDADRLRDWGFMQSESRESPFEALAASAEQRAVERALASLPAPEREVLLLCAWSG
                     FEPAEAAVIIGIRADAVRQRLSRARAKLRERLRHMELSFA"
     gene            7275..7514
                     /locus_tag="VFQ35_28310"
     CDS             7275..7514
                     /locus_tag="VFQ35_28310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934654.1"
                     /translation="MNTEDRLRDLPTYDVDAPVAARIRARAHAALPSESTARTIVALG
                     ARNDWFYRIVEPAALIGLGIAQLFWTVHDTFALFR"
     gene            complement(7473..>8706)
                     /locus_tag="VFQ35_28315"
     CDS             complement(7473..>8706)
                     /locus_tag="VFQ35_28315"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934655.1"
                     /translation="VGSYGDVIPFVAIAEALRKRAHAVRVCSWRPFERLFRERNIEFS
                     AVGSDFDLHGYSKSVIASAMSNPDPGTMLEGAFDLFVAFEARRRFEECRAAMVGADAA
                     VCNALDMPAQAAAESVGIPWFVWTSKPLDRDTLPLSDAHFADWDRRANFLLERETGVQ
                     LRARAFRNLSPYETYFGVSEALLPGFDPPPRCFVTGTCFLDDFVPAIPEGVLEFLNGG
                     DAPLVVTFGAFGELLPDEAFGAVIEAVRRTNVRAIVHAKRRDSLAREGDGLLFVESLP
                     YAAVFPRARAVLHHGGAGTTATVCRAGVPSATLSYIDSDQAYWAAQIHGLGVATEPLM
                     LRELDVEALHARLNTALNDGGMRRRAVALAPRLRSEDGAANIVSRLESFERSRRAPPH
                     VSVQPSAEQRERIVHGPK"
BASE COUNT         1514 a         2827 c         2773 g         1592 t
ORIGIN      
        1 cagcgctttg ggtacggcac gtaccgcacc cgcgtcgctt ttggcgattg cgcgagcaac
       61 gaagaagcga cgcaagcagt gctcggctac ttcagcgacg gcgccgacca caacggaaac
      121 ggaattcgcg acgagaccga gatcgatttc caagtgctct gcggcgcgcc ttcgctggtt
      181 tatctcaccg ttttcactga ctacgaagag cgaccctctg gcacggtgtt ccgcaagctg
      241 agtcacgtca tcgatttcgc gaccggtcgc acttacgaca caccgtcgcc gaacgaagac
      301 gccttcgccg cggggcccat cgatccgacg ctggtgctgg agctgttttc gccgggtcgt
      361 ttctacgagg tcggcttcga gtggcacgcg gcccagattc gctttttcat cgacgcgggc
      421 gcgggcgaaa agacactgtg gacgctcact gatccggcgc gtatcccgac cggttcggtg
      481 cagctcgttt acaatgcgtg gcacccggag agccactggt tcccgctctc gagccccgcc
      541 gattaccccg ccgacgacgt ggtgatgtcg atcgattggt tccgctttta cgcggagtga
      601 gggcgccgtt ggtcacggat tgaagtaggg gtcgttcgcc atcttcttga aagccgcttc
      661 cgcggccgga gacgaactca cgcgccagtc ggtctccttg tcgacgtcga accagagcac
      721 cccgcgaatc tgtgggtact tgttcttcaa ggtcggaatc atcgcgtcga tccaggcgcc
      781 cttgttgccg cccgactctg ccgacgccat ctcgccgatc atgatcggtt tgttcttcgc
      841 ggcaagcttc ggataaatcg cttggaacac ttgttcgaag ctctgccagc ctccaccatc
      901 ggtatcgccc caattgtacc cgtcgacgca catccaatcg acgtagtcgt ctccggggta
      961 gtaatcgagt gcttcgttcc aggcggcgcg tggctcgtcc gtcacgttcg ggcagaacgc
     1021 ccagatcgcg ttcgtcgcgc ccgcgtcctc gaaagcgtcg tgaacttttc gatagacggt
     1081 gaggtacttg gcggcggatt cgccgttttg cgcgccgccc caaggcgacc aatcaccgtt
     1141 catttcggca ccgaaatcga tgaacagcgg cttcccgagt gccttggtgt cgcgcgctcg
     1201 ctgttgcagc atgctgtcgt acttgccggc gatgatgtcg tcgagctcgc caccgaatag
     1261 ctcccaattg acgaacggaa tgcgtcccgc cgacagatcc ttggccgttt cggcgcgagt
     1321 gaagtcgtcc tcccaagcgt agtaggtgag atgtagcggc aacgagcgcc cgagcttcgt
     1381 ggcggtcgcg gcgatgctcg cgtcaccgta gtaaagccct agtagcgcgc cgctctccgg
     1441 caccagcaga tttcgcccgc cagagccgcc gctcgcttga gtcgagcctc ccgagccgcc
     1501 gaggccgagc gcaccgccgc tcgcctgacg accacccgtg gcggtatcac cgccggttcc
     1561 ggcgcggccg ccggtgcccg tgcgaccgcc acttcccatg cgaccgccag cgccgagcat
     1621 gcctccactc gcatcggtgc ctcccgcgcc ggagcctcca actccggagc ctcccgaact
     1681 cgccttccca ccggattgcg cggacatgcc gccggaagat tggcggccac cttcggtatt
     1741 cgaggtccca gctccgcctc cttgcgacgc agtcatgccg ccgctgcctt gctcgcgcgc
     1801 gcctccggat gagctggatt gcttggcgcc gccaaaaccg aaaccgccag agccgccgcc
     1861 gctgccctca ttactgctgc acgcctgcat ggcgagcccg agcgcgaacg cggtggaggt
     1921 gatgacgaaa gccgaaccga tggaggtgtt cgtggtcacg ttcccttgga gtcgcgtcga
     1981 aggtcgcgct atttgaaaaa gtgacggact cggttcgaat ttcggcgcgt ctgagcggct
     2041 gcgaccgtgg tcggaatttt cctgtcagtg gctgggctta cgaagccgct aggcggcccg
     2101 caatggacag gagctcggtt accttcgcat cgagtgattt cgtatcgaac ggcttggcca
     2161 gatacgcgac gcctttgcgc tcatcgctcg ggggcagctc ttctccccag ctgtagccag
     2221 agatgaagag caccgccatg cctcgttgtt ccggagccag cgtcttcacg agctccgctc
     2281 caccaaggtt cggcatgacc acgtcggtga tcagcaaatc gatccggttg ccgtgagaac
     2341 gtgccaacgc gagcgctcgc gccccgtctt cggcttcgag aatccggtga ccgagacggg
     2401 ccaggttgcg gcgcatggtg gaacgaacca tgggttcgtc ttctgcgatg agaatcgtcg
     2461 cgacccgagc gctctcgctc gcagttccct tgatcagcgg cgcgggcgag tgctcgacgg
     2521 cctcgttgat gggaaggtga acgaagaatc gcgaaccatg gccgagcttg gattcggcgt
     2581 tcacgcgccc cccgctctgt tggatgatgc cgtggacggc agcgaggccg aggccatggt
     2641 ccgggctccc gaccttggta gagaagaacg ggtcgaaggc gtggcgcagc gtatcctcgt
     2701 ccatgccgat gccggtatcg tcgacacgta ggagcgcgca cggacgcagc tccgcgggct
     2761 cgccgagcaa tgcatcggtt cgttgtgtca cgattcgcag ttcgccccca ttcgccatcg
     2821 cctcgcccgc gttgctcacc aggttgatca agacgtgtcg gagtcgcgat acatcggcca
     2881 agacgcggag tggcctcggg ctcaggtcga aagtcacgtt cactcgcgcc cccgctagcc
     2941 gaaccagcag tggcctgatg tcgacgatca cctcgttcaa gtcgacgggt ttggactcga
     3001 ggtgcagccg gctgctgaac gccaaaagcc gctgaacgag ctcggcgccg cggcgcatgg
     3061 aatcgagcgt ctcgaggagc gctgcattgc cgaccgcgtc gaggtgctgt gtgtctttca
     3121 gcacgtccac attggtcatg ccgatggtga ggagattgtt gaaatcgtgc gcgatgccgc
     3181 ccgcgagccg gccgaggctc tccatccgtt gagcatcgcg caaagccgct tcctgcttct
     3241 cgatctctgc cgtgcgctca cggacgcgtt gttcgagcac gcgattcgcg ttctccagaa
     3301 cttcttgcga gcgcgacagg cgctcgagta agtccgcgtt ctcgaatgcg aggtggaatg
     3361 ccttcgagat cgatgcgtgg ttcgtgcgcg ccacgcgatc gatgaagatc ccgtaggcca
     3421 gcaacatgag gcccatcgcg gtgtgcggcc aatcgcccac ggcgaacgtg cgcacgatga
     3481 atggcccgag cgatgccaag cagtacccgc gaaacgtcgg caggtgcgag gaccacgtgc
     3541 cggcggcggc ggcggtcatg ccgccaatcg caaacgaaaa cagcgcctcc gtcatggcgt
     3601 gcgtcgcgtc gaagaaaacg agcgtcgcca ctccccagag cgccccagct gctatcgatc
     3661 ctgcaacgaa gcgctgcccc caaacctcga gctccttcgg ttgcggccgc gtggtgtcga
     3721 agcgcctgcc gagcgcgacg cgcccagccg cgacggcaat cgtcaatgca acccacgccg
     3781 cgagtagtgg tcgtggggtc gaggtgagga tccaaagggg accggcgacg agcgccgaga
     3841 cagcgacatt ggcccaaagg acggcgcggt tctgcccgaa gagcatgcgg atctgctccg
     3901 cgcgcaccgt ctcgcccaag gccatctaac caatatgcca gacgcggctt gctccatgaa
     3961 ggtgtgacgt ctcgcgactc gcaccgctct tcggagattg attcgcacgc tcttcagttt
     4021 gaaaggcggt gcgcccaggt tcgtgcgaac gcgaaagccc ggtacgtaat tgcaacgcgc
     4081 aacgtttgtg caacgcccta cgttgcaagc gcgaaatgcc tacacgcggg ctgctgatcg
     4141 gaaagccagg cgcattgcac aacgagacag gagagcgcaa cgggcacgaa cgcaacgcaa
     4201 ggtggagtca tgaccgagcg ggggctcccg tttgcttcga caagttggct cgcgcgtcga
     4261 gctgcgcttg ctgcccgcct ccaatcgcac gcgacggaca gcgaattcgt gggctccgtt
     4321 gacgcagcga atcctcgcga gcgcgatcgt gcggcgtctt gcggggattc gcgcacgaac
     4381 acctcttgtg tgaggggtgg cggtattcgc cgcggctggg gcccgcatcg tgctccggcg
     4441 tgacgagccg cacaatgtgt tactccgtcg ggtcgccccg acgccgccga aaggtttctt
     4501 ccctgacggc cggtcgcgcg gcttcgaatc ccagctcact ccacgccaga gcattcatgc
     4561 cgaagacatc cctcgcccga gccgagcccc ttgcatttgc ggcaatgcga atcgtaagtg
     4621 gtgtcctctt tctctttcat ggcctccaga agttgatcaa ttggcctcct ggcgggcacc
     4681 ccgttgcgct tgcgagccaa gtcggcgtcg gtggcgtgat tgagctcgtt tgcgggacgc
     4741 tcatcgcttt gggttggcgc acacgcggcg cggccttcat cgcttcgggg atgatggcag
     4801 tcgcgtactt tcaatttcat tggaagctcg cgattgcgaa cgtagcgtgg gcaccagtcg
     4861 tcaatcgcgg tgagctcgcg gtgatttatt gtttcttgtt tttgttcatc gcgacccacg
     4921 gctcaggcat cgcttcggtc gacaagcttt tggcgcgacg ctgactctgc tgatcgcgtg
     4981 gcgctggcgc gttgacgatg cctcgttcga gatcttgacg ctttgcagcc gtcgcagcgc
     5041 tgcgaacccc agatttcagg ctgaattgcc cagagcacgc gatggtcgag gtgcgacgcg
     5101 ggcgagggcc agcggtgctg tgtccgctcg ggatgccttg tcacgctctt tgcagagtcg
     5161 cgcctcgcca tgtcgacgaa gcgccatttc gaaactgcaa tagagctcgc cgcaaacgac
     5221 gtcgcccgcg acgtctcgcc agcctcggcg tctgccgcga ccaagcgtca aagctttcct
     5281 cgcatccaca ttcaagaaga agacgagcac gtcgtcgtgt tggccgtcgt accggggttt
     5341 acgcgcgaag acttgcggat cgacgtccag cgcgacgtgc tcgcgttgag cgggcgtgtt
     5401 cgttgccagg ttccccaggg cttcaccgcg ctgcagaagg gccgtacgca cgaggacttt
     5461 tcccggcaca tcaagctgat gaccgacgtg agccggggag aaaccgaggc gcgcctcgaa
     5521 cgcggcgtgc tcacggtgcg catgcgcaag cacacggcgg cgttgcgcac gcccattcca
     5581 gttcgcgtcc cttgaacagg attcatcatg cgagcagcac tcgaacgtac tcaagagcct
     5641 tcgaatttac cccatcgccc gagcgcggcg ccgcacgcat cggttgcgcc cgatacgtct
     5701 caagtcgacg attcgtggta tcccgacgct ccgcgctatt cgatgatcct cgatgcgcgc
     5761 gcgcgagcga cgaacgcccc gccgacggcg cgagcggcga cgctgctccc gaggccggtg
     5821 aattcgctct cgacgcctcc tcccccaacc accgagaagc cgcgtgagct cgccttcgac
     5881 gtctacgaga gcccgcacgc ttggctcatc ttggtggatg cgcccggagt cgaccccgat
     5941 tggatttcgc tcagcctcgg tagtcgcgcg ctgcacctcg aggcttcggt tccgacgtgg
     6001 accaagcaag cggggctcaa gccgggacgc tacgagatga ccgtcgaggt tccggcttgg
     6061 gtcgaagccg acgcgatcga cgcagcggtc gagaacggcc tcatgcggat ccgtatcgcg
     6121 gcgaccaaga gtggcccgcg caccatcgcc atcctcggcg aagagtcgtg agccaccctc
     6181 ggcgttggcg cggagccgtg tgaaccggcg cgcgcggaag ggaacgccgg ctcagctagt
     6241 catttgttca cggccgagtt tgcgctgccc caccgccacg accaaccacg cgagcgcgag
     6301 caacgtgaag ccgaggctag agggtgcgat cgcttcggcg atgccctgca tgacgatgag
     6361 atgaatttgc gggctgttcg cccactcggg gttcgcgggg accttcgcgc cgacggcggc
     6421 gatgttgacg ctgacccccg taatgatcga aaagacggtg gcgcggctga aggagcgcac
     6481 aatgtcggcc tggcgcacgt tgggctgtct gagaaagctg acgctcatgg ccagcgtgac
     6541 taggccgaac gccagcacga accacatcga ccagccacct tcgagcatga attcaacgaa
     6601 tctcatgact tgagctcctc ggagaacgcc tcttcgtgaa tgttccgatc gacacgacgc
     6661 tcgtggaggc ccagcgtgac gaatagtcgt cacgccgagt cgattcgtgc gtcgatcgta
     6721 gcggtggagc tcgccgaacc cgaattgctg cgcgccctag gccggggcga ccccaacgcc
     6781 ttcgaccagg tgtacgagcg cttgcgggct ccgcttcact ccttcctggt tcgcctgagc
     6841 accgaccgag agcttgccga agatctgctg caagagactt ggctacggct ggcacggcac
     6901 gcggcctcgt tgccacacga tacgaacttg cgggcgtggc tctttacggt cgcgcggaac
     6961 ctctaccgga gccatcggcg ctggtcgttg ctcgacgccg ataggctgcg cgactgggga
     7021 ttcatgcagt ccgagtcgag ggagtccccc ttcgaggcgc tggccgcgag cgcggagcag
     7081 cgagcggtgg agagggccct cgcgagcctt cccgcgcccg agcgcgaggt cctgcttctt
     7141 tgcgcctggt ccggcttcga gccggcagag gcggccgtga tcatcggcat tcgcgcggat
     7201 gcggtgcggc agcgcttgag ccgcgcccgt gccaaacttc gcgagcgttt gcgccacatg
     7261 gagctgagct tcgcatgaac accgaggacc gcctcagaga ccttccgacc tacgacgtcg
     7321 atgctcccgt cgcggcgcgg atccgcgcgc gcgctcacgc tgcgctcccg agtgagtcga
     7381 ccgctcgcac aattgttgcg ctcggcgcgc gcaacgactg gttttatcgc atcgtcgagc
     7441 cagcggcgct gatcgggctc ggcatcgctc agctattttg gaccgtgcac gatacgttcg
     7501 cgctgttccg ctgacggttg cacggacacg tgggggggcg ctcgccggga gcgctcgaac
     7561 gactcgagcc gcgacacgat gtttgcggcg ccgtcttcgc tccgcaagcg aggcgcgaga
     7621 gcaacagcgc gtcgccgcat gccaccgtcg ttcaacgccg tgttcaggcg agcgtgcaga
     7681 gcctcgacgt cgagttcgcg cagcatgagc ggctcagtcg cgacgccgag gccgtgaatt
     7741 tgcgccgccc agtaggcttg gtccgagtcg atgtagctga gcgtcgccga cggcacaccg
     7801 gcgcggcaaa cggtcgccgt cgttccggcg cccccatggt gtaacaccgc gcgcgcgcgt
     7861 ggaaacacag ccgcgtatgg aagcgactcc acgaacagga gcccgtctcc ctctcgggcg
     7921 agcgaatcgc ggcgcttggc atggacgatc gcgcgcacgt tggtgcgacg aaccgcctcg
     7981 attacggcgc cgaaggcttc gtccggcagg agctctccga aggcgccgaa cgtcacgacg
     8041 agcggcgcat cgccgccgtt caggaactcg agcacccctt ccgggatcgc ggggacgaag
     8101 tcatcgagaa agcaggtgcc ggtgacgaag cagcggggag gcgggtcgaa gccgggcaac
     8161 agcgcctccg aaacgccgaa ataggtttcg tacggcgaca aattgcgaaa agctcgcgca
     8221 cggagctgca cgcccgtttc tcgttcgagc aggaagttgg ctcttcggtc ccagtcggcg
     8281 aaatgagcat ccgagagggg cagcgtgtca cggtcgagcg gcttcgacgt ccatacgaac
     8341 caggggatcc cgacgctttc ggcagcagct tgcgcgggca tgtcgagcgc gttgcacacc
     8401 gccgcgtcgg cgccgaccat tgctgcgcga cactcttcga agcgacggcg cgcttcgaac
     8461 gcaacgaaca agtcgaaggc gccctcgagc atcgttccag ggtctgggtt gctcatcgcg
     8521 ctcgcgatga cgctcttgct gtacccatgc aggtcgaaat cgctgccgac ggcgctgaac
     8581 tcgatgttgc gttcgcggaa aagccgctcg aacggacgcc agctgcatac gcggacagcg
     8641 tgagcgcgct tgcgcaacgc ctcggcgata gcgacgaacg ggatgacgtc gccgtagctg
     8701 ccgacg
//
LOCUS       DASTKS010001322         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1332211, whole genome shotgun sequence.
ACCESSION   DASTKS010001322 DASTKS010000000
VERSION     DASTKS010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332211"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..535
                     /locus_tag="VFQ35_28320"
     CDS             203..535
                     /locus_tag="VFQ35_28320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934656.1"
                     /translation="MKPDSSSSLRTRLAFTLALALALPVALGIAGAAVVVGAVLYISA
                     ERRRRRSPQYLLERWLGEPRDSALRSALKGAARALAVKALQAAARRAIDRLGEAHETS
                     PALMAPRP"
     gene            861..1676
                     /locus_tag="VFQ35_28325"
     CDS             861..1676
                     /locus_tag="VFQ35_28325"
                     /inference="COORDINATES: protein motif:HMM:NF012535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HET9934657.1"
                     /translation="MSGGTSSGGQSASGGRSGAPKGGGSGGAATSTSGGATSKGGAAA
                     SSGTTGKGGATSKGGATAAGGANGGAGTAGTASSIDCLKDGDAKTTLAFVNRCSQSVT
                     FAGSDIKGGSLASGAAQCVDVGNASDAISSKRYWGYTGDDPGAGKYSLAEFTFNTDFQ
                     DFDWYNISYVDAFNLPMQIVPVARTNCKSLTCAEDFLSGCPDVGRFEKGGKLVACVNP
                     SRDDGANPVAKYFESCDESYAWSGDDQNGSDPSPVRACAGEDFEIVFCPAGKP"
     gene            1673..>2692
                     /locus_tag="VFQ35_28330"
     CDS             1673..>2692
                     /locus_tag="VFQ35_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159397871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934658.1"
                     /translation="MNVTPFVTFAVFVLATACQSGGSTDGGLGGAQSSGGAFTETGGD
                     TSGSGTSSSGGKASAGGATSMARGGAANGGTSATPRGGTSGGGAAAQGGTSAARGGTS
                     NAAGAPSTAGALGAYPVTPSTVTRGGTLTFTNIGAAGWWPRRIDRAAGDPACNYKDGT
                     DTWGGHCCMKQNATTSTNLSPFDEEMVLILKAIDIKQLAVYQPKAGGSDFARVTAWDR
                     RSNAAENLWFTQKGDGAAQFLGDLTKNDCVGYVSQAPRIDCGDGRDYYCPNDPGVLHR
                     GFSGSKLVVFLGNMDFEDAGVAKCDGGAGGAPQPWVAFVASELVRDGGRKWNGACNCY
                     SKTGSV"
BASE COUNT          473 a          862 c          942 g          415 t
ORIGIN      
        1 gctgcgccgt cggcaccatc aagagccgaa tcggccgggc gcgcaaggcg ctgcaagccg
       61 agttcgaggg cgttcagccg gaacgccggt ccgtgcggcc gcgcgcccgc atccggcacg
      121 cacccgtcgc ccgggcgcag cagcaagcgc aaacaatgtg gtccgactct gacgaatgag
      181 gcggctacgc cggtggctgc gcatgaaacc ggacagcagc agttcgctcc gcacccggct
      241 ggcgttcacg ttggcactgg cgctcgccct gccggtggcg ctcggcatcg ccggagccgc
      301 cgtcgtcgtc ggagcggtgc tgtacatcag cgccgagcgt cgacgccgac gctctcctca
      361 gtatctgctc gagcggtggc tcggtgagcc ccgtgacagc gcgctgcgca gtgcgctcaa
      421 aggcgcggcg cgagcgctcg cggtgaaggc actgcaggcc gcggctcgtc gagcgatcga
      481 tcgcttggga gaggcgcacg agaccagtcc cgcactcatg gctcctcggc cgtagcaggg
      541 gttttccaac gccctgctcg aggggctctc ccctaccgct gcattcggcc gaaccgagtg
      601 cgcgcccgtg agtgcaggcg ggctcaccgc tcagcattca tcacatcatg gccgagctcg
      661 atcgagcacg tgtcgccagg ggtcgcaccc attgcgggct ctgagcacgt cgagtgcttg
      721 ccccggaccc ttcgcgacga gaagatggtc gagtgaggct cggtgttttt ggtgcgatcg
      781 catctctcgc ttcgatggtt gcgtgcggtt cgtccgatgg gagcgcttcg gcgaacggtg
      841 gagggccgtc ccagagtgaa atgagcggcg gaacgagcag cggcgggcaa agcgcgagcg
      901 gtggccgctc gggcgctcct aaaggtggtg gctctggcgg cgccgcgact tcgacgagcg
      961 gcggcgcgac cagcaagggc ggggcagccg ccagcagcgg cacgaccggc aaaggcgggg
     1021 cgaccagcaa aggcggggcg acggctgctg gcggagccaa cggcggtgca gggacggccg
     1081 ggaccgcttc gagcatcgac tgtctaaagg acggcgacgc gaagacgacg ctcgcgttcg
     1141 tgaaccgctg ctctcaaagc gtgacttttg ccggcagcga catcaaaggt ggctctctcg
     1201 cgtcgggtgc cgcgcaatgc gtcgacgtcg gcaacgcgag cgacgccatc tcgtccaagc
     1261 gttactgggg atacaccggt gacgacccgg gcgcgggtaa gtactcgctc gccgagttca
     1321 ccttcaacac cgactttcaa gacttcgact ggtacaacat cagctacgtc gacgccttca
     1381 atctgcccat gcagatcgtc cccgtcgcgc gcacgaactg caagtcgctc acgtgtgcag
     1441 aagacttttt gagcggttgc cccgacgtcg gccgcttcga gaagggcggc aagctcgtcg
     1501 cctgcgtgaa cccgagccgt gacgatggcg cgaacccggt cgcgaagtac ttcgagagct
     1561 gcgacgaatc gtacgcttgg tcgggagacg accaaaacgg tagcgacccg agcccagtgc
     1621 gcgcgtgcgc gggcgaagac ttcgagatcg tattttgccc ggcaggtaaa ccgtgaacgt
     1681 cacgcccttc gtcacctttg ccgtcttcgt gctcgctaca gcttgccagt ctggtgggag
     1741 caccgacggg gggctcggtg gagcgcagag cagcggaggg gcgttcaccg aaacgggcgg
     1801 tgatacgagc ggcagtggca cctcgtcgag cggcggcaaa gccagcgctg gcggagcaac
     1861 ttcgatggcg cggggcggcg cggccaacgg cggcacgagc gcaacgcccc gtggcggaac
     1921 gagcggcggt ggcgcggccg cgcaaggcgg aaccagcgcg gcgcgaggcg gcacgtcgaa
     1981 tgcggccgga gcgccctcga ccgcgggcgc gctcggcgca tacccagtca cgccgtcgac
     2041 agtgactcgc ggcggaacgc tcacgttcac caacatcggg gcggctggtt ggtggccacg
     2101 acgcatcgat cgcgccgccg gcgaccccgc gtgcaactac aaggacggaa cggacacgtg
     2161 gggcgggcat tgctgcatga agcagaacgc gaccacgagc accaacctct ctccattcga
     2221 tgaagagatg gtgctgatct tgaaagccat cgacatcaaa cagctcgccg tctaccaacc
     2281 gaaggctggc ggttctgatt tcgcgcgagt cacggcgtgg gaccgccgca gcaacgccgc
     2341 cgaaaacctt tggtttactc aaaagggcga cggcgcggcg cagttcctcg gcgatctcac
     2401 caagaacgac tgcgtcggct acgtgtcgca agcccctcgc atcgattgcg gcgatggccg
     2461 agactactat tgtccgaacg atccgggcgt cttgcaccgc ggcttttcgg gctcgaagct
     2521 cgtcgtcttt ctcgggaaca tggacttcga agatgcgggt gtcgccaagt gtgatggcgg
     2581 cgcgggtgga gcgccgcagc cctgggtcgc attcgtggcc tcggagctcg tgcgggacgg
     2641 cggacgcaag tggaacggcg cctgcaactg ctattcgaag acgggcagcg tt
//
LOCUS       DASTKS010001323         5657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_883420, whole genome shotgun sequence.
ACCESSION   DASTKS010001323 DASTKS010000000
VERSION     DASTKS010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883420"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="VFQ35_28335"
     CDS             <1..1734
                     /locus_tag="VFQ35_28335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9934659.1"
                     /translation="PRSPKEGDGRASNLESVRVDVELLDRLMDLAGELVLTRNQIMQL
                     QNTLGESALTAASQRLNLVTIGLQEGIMRMRMQPIDHVLSKFPRMVRDVARACKKLVR
                     LEMDGKNTELDKTLIEAIKDPLTHLLRNAIDHGIEGPEQRSRAGKHPEGRILVRAAHR
                     SGQVHIEIIDDGAGIDVERVRQKAISRGIVSAERAEMMSEGEVVNLIFLPGFSTAETV
                     TNVSGRGVGMDVVKTNVERIGGAIEIETRLGRGTTFRIKVPLTLAIVPALIVNAGSAR
                     YAVPQVSLQELVLVDTERTRQRIEQVHGAPVYRLRDKLLPLVFLNEQLGYETREECFA
                     KRKVHVAVLLAGGRQFGLVVDRVDDQQEIVVKPLAGALKPVNVYSGATILGDGRVVLI
                     LDVVGIAQRANLVAEAREDSVAQTQLDKEVAKREQMTSLLLLRGPDDSRVAVPLERVA
                     RLETVPVSQLEEAGGTEVLQYGNGIMPIVHLSNLLPERRLRPRHEDVEPPTPDYVNLV
                     VLSVRGSQVAMAVHDILDVIDAEIVIRSPGSRAGVLGTLVLQQRVTELLDLDWVVGQA
                     GFQPDGYQEAV"
     gene            1731..2153
                     /locus_tag="VFQ35_28340"
     CDS             1731..2153
                     /locus_tag="VFQ35_28340"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9934660.1"
                     /translation="MTTVQFCTFELGSLLLGLEAKLVQEVLRAQPVTEVPLAPHAVRG
                     LMNMRGQIVLVVDLRRRLGLPEPVGDVRRVNVLVRTEDGLVSLLVDRVGDVLEISPEE
                     FEAVPDTLDAEARQLLRGAYQLPERLLLQLDAQRAAAI"
     gene            2167..3765
                     /locus_tag="VFQ35_28345"
     CDS             2167..3765
                     /locus_tag="VFQ35_28345"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HET9934661.1"
                     /translation="MVNIQNFTIKGRIALVALCAATTIVVTVLISYIALESSRVGSDS
                     YRRVVEGKDLIADVLPPPALILESYLVANQIVLEKDEHARGQLFDRCRQLKSDFESRH
                     IYWRERVSEGPTRRLFLEDSYRTAVQFFVAVEGPLYQASREGNAEKMRAILDQTLTPK
                     FQEHLGFIRQVVTSVERDVVLQEDVAKGAARLGTSVVVGFALLSIVLVLLVGRGVANS
                     LRQTFAESRRVFHSLANGDLTARFEHNGGDEIGTMSESVNQGLEAMVAAMRAVGQQIE
                     ALATASEELTAVSQQMSANAEETAAQASVVSASSEAVQRTIQSVATATEQMNAGIRDI
                     TKGSGETAGVVSKAVRLAESAGSAMQRLAESSAGIGKVVEVISSVAEQTNLLALNATI
                     EAARAGEAGKGFAVVANEVKELAAETSRATDDIARKIQAIRGDASSAVDAIGEIRSVI
                     TRMSELQASVVAGLEEQTTATGEISRNLADGVRGASEITSNIGGVAQAARGTSAGAVD
                     TKSAAEELSRMAAELRRVLGGFAY"
     gene            3774..4145
                     /locus_tag="VFQ35_28350"
     CDS             3774..4145
                     /locus_tag="VFQ35_28350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934662.1"
                     /translation="MRAIVVDDSRTMRTLLKTVLREAGFDVVAEAENGREALEALART
                     GPVELMLLDWNMPVMNGYDLLLEIRSNSKLSRMRTMMVTTETEASQILRALEAGADEY
                     LIKPFGRHAIREKLELMGLFE"
     gene            4142..5251
                     /locus_tag="VFQ35_28355"
     CDS             4142..5251
                     /locus_tag="VFQ35_28355"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9934663.1"
                     /translation="MKADRKIRILVVDDSALVRKIVTDALNGEGDMEVVGVASNGKIA
                     LEKLEFLKPDAVVLDLEMPEMDGLTTLKRLRKDFRLLPVVVFSTLSERGAKITLDALT
                     AGASDYLCKPSGTGSLAASKDLLHGVLAPKLRALALRNVAVAPRNAPAAPAPSPMPAM
                     VPARRVNEPIEAICIGCSAGGPQALAEVIPKLPASLGVPVMVVQHMPPLFTKFLAERL
                     AQTAKLRVIEAADGMSVEGGSVYVARGGEHMRIVLRGARPVLVLDTGELEHGCRPAVD
                     PLFRSAAALYGQHLLAVVMTGMGVDGTQGARAVQKAGGRVWAQDEQSSAVWGMAGSVV
                     KAGVVERIFKLSALAEDLARSVDVSRRPVAGGARG"
     gene            5253..>5657
                     /locus_tag="VFQ35_28360"
     CDS             5253..>5657
                     /locus_tag="VFQ35_28360"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9934664.1"
                     /translation="MALLPGELGVVQRLVLERSAIVLGDEQGYLVESRLANLARESGC
                     DVPTLVARLGREPHGGLHERVVEAMTTNETSFFRDRHPFAAVQERALPELMLMRGRER
                     KLRVWSAACSSGQEAYSLLMLMWEAFPMLKLWD"
BASE COUNT         1006 a         1753 c         1950 g          948 t
ORIGIN      
        1 ccacgctcac cgaaggaggg cgatggtcgt gcgagcaatc tggagagcgt tcgcgtcgac
       61 gtggagttgc tcgaccggct catggatcta gccggcgagc tcgttctcac gcgcaaccag
      121 atcatgcagc tgcaaaacac gctcggtgag tcggcgctca ccgccgcgtc gcagcgcctc
      181 aacctcgtca cgatcggtct tcaagaaggc atcatgcgta tgcgcatgca gcccatcgac
      241 cacgtgctca gcaagttccc gcgcatggtc cgcgacgtgg cgcgcgcatg caagaagctc
      301 gttcggctcg agatggacgg caagaacacc gagctcgaca agacgctgat cgaagcgatc
      361 aaggatccgc tcacgcacct cttgcgtaac gccatcgatc acggcatcga aggacccgag
      421 caacgatctc gcgctggcaa gcaccccgag ggccgcatcc tcgtgcgcgc cgcccatcgg
      481 agcgggcagg tccacatcga gatcatcgac gacggcgcgg gtatcgacgt cgaacgcgtt
      541 cgccagaagg cgatcagtcg cggaatcgtt tcggccgagc gcgccgagat gatgagcgaa
      601 ggcgaggtcg tgaacctcat cttcctgccc ggtttctcca ccgccgagac ggtaacgaac
      661 gtgtcgggtc gcggcgtcgg catggacgtc gtcaagacca acgtcgagcg catcggcgga
      721 gcaatcgaga tcgagaccag gctcggacgc ggcaccacgt tccgcatcaa ggtccctttg
      781 acgttggcga tcgtcccggc gctaatcgtc aacgcgggaa gcgcccgtta cgccgtgccc
      841 caggtcagct tgcaagagct cgtgctcgtc gacaccgagc gcacgcgcca gcgcatcgag
      901 caggtacacg gcgcgccggt ctatcggctc cgcgacaagc tcctgccgct cgtgttcttg
      961 aacgagcagc tcggctacga gacgcgcgag gagtgcttcg cgaagcgcaa ggtgcacgtg
     1021 gcggtgctgc tcgccggcgg tcgacagttc gggctcgtgg tcgaccgcgt ggacgatcag
     1081 caggaaatcg tcgtcaagcc tctggccggt gcgctcaagc ccgtgaacgt gtactcgggc
     1141 gcgaccatcc tcggcgacgg acgcgtggtg ctgatcctcg acgtcgtggg catcgctcag
     1201 cgcgcgaact tggttgccga ggcccgagaa gattcggtgg ctcagactca gctcgacaag
     1261 gaggtcgcga agcgggagca aatgacctcg ttgctcttgt tgcgcggccc ggacgactcg
     1321 cgggtggcgg tgccgctcga gcgcgtggcg cgactcgaaa ccgtgccggt ctcccagctc
     1381 gaggaggcgg gcggaaccga ggtgctgcag tacggcaacg gcatcatgcc catcgtgcac
     1441 ctgtcgaatc tgctgccgga acgccgcttg cgtccgcgtc acgaagacgt cgaaccgccc
     1501 actccggact acgtcaacct cgtcgtcctt tcggtgcgag gctcgcaagt cgcgatggcg
     1561 gtccacgaca ttctggacgt gatcgacgcc gaaatcgtga ttcgtagccc gggctcgcgc
     1621 gcgggggtgc tgggcacgct cgtgctgcag cagcgcgtca ccgagctctt ggatttggat
     1681 tgggtcgtcg gacaagccgg atttcagccc gacggctacc aggaggcggt atgacgaccg
     1741 tgcagttttg caccttcgag ctcgggagcc tgctcttggg gttggaggcc aagctcgtcc
     1801 aagaagtgtt gcgtgctcag ccggtcacgg aggtgccgct cgcgccccac gcggttcgag
     1861 gcttgatgaa catgcgcggc cagatcgtgt tggtggtcga cctacggcgc aggctcggcc
     1921 tgcccgagcc ggtcggcgac gtgcgacgcg tgaacgtgct ggtacgcacc gaagacggcc
     1981 tcgtcagcct gctcgtagac cgggtcggcg acgtcttgga gatttctccg gaggagttcg
     2041 aggcagttcc ggacaccctc gacgcggaag cgcgtcagct tctgcgcggc gcctatcaac
     2101 tgccggagcg gcttcttctt cagctggacg cgcagcgcgc cgcggcgatt tgagagacaa
     2161 acgaacatgg tcaacatcca aaacttcacc atcaagggcc gaatagcgct ggtcgcgctt
     2221 tgcgccgcca cgaccatcgt cgtgacggtg ctgatcagct acatcgcgct cgagtcgtct
     2281 cgcgtcggca gcgacagcta ccggcgcgtg gtggagggca aggacctcat cgccgacgtg
     2341 ttgccgcccc cggctctgat cctcgagtcg tatctggtcg cgaatcagat cgtcctcgag
     2401 aaggacgagc acgcgcgcgg ccagctcttc gatcgctgtc gacagctgaa gagtgatttc
     2461 gagtcgcgtc acatttactg gcgggagcgc gtctccgaag ggccgactcg acggctcttc
     2521 ctggaagatt cgtatcggac ggcggtgcag ttcttcgtcg cggtggaagg tccgctctac
     2581 caggcctcgc gcgagggcaa cgccgagaag atgcgcgcga tactcgatca aaccctcacg
     2641 ccgaagttcc aagagcacct cgggttcatt cgccaagtcg tcaccagcgt cgagcgcgac
     2701 gtcgtgcttc aagaagacgt cgccaagggg gccgctcggc tcggcacgag cgttgtcgtt
     2761 gggtttgcgc tcttgagcat cgtgctcgtg ctgctcgtcg ggcgcggcgt cgcgaactca
     2821 ctgcgccaga cgttcgcaga gagccgccgc gtgttccact ccctcgccaa cggcgacctc
     2881 accgcgcgct tcgagcacaa cggaggggac gagatcggca cgatgtccga atccgtgaac
     2941 caaggcctgg aggcgatggt tgcggcgatg cgagcggttg ggcagcaaat cgaggccctc
     3001 gctacggctt cggaggagct gacggcggtg agccagcaaa tgagcgccaa cgccgaggag
     3061 acggcggccc aagcgagcgt cgtttcggct tcgtccgagg ccgtgcagcg cacgattcaa
     3121 tcggtcgcga ccgccaccga gcagatgaac gcgggcatcc gcgacatcac gaagggaagc
     3181 ggcgagaccg cgggcgtggt ttcgaaggca gtgcggctcg ccgagagcgc gggctctgcg
     3241 atgcagcgcc tggccgagtc gagcgccggc atcggcaagg tcgtcgaggt gatcagctcc
     3301 gtcgccgaac agacgaacct cttggcgctg aacgcgacga tcgaagcggc gcgagcggga
     3361 gaggccggca agggcttcgc cgtcgtcgcg aacgaagtca aggagctcgc cgcagagacg
     3421 tctcgcgcca ccgacgatat cgcccggaag atccaggcga tccgtggtga tgcgtcgagc
     3481 gccgtggacg cgattggtga gatccgcagc gtgatcaccc ggatgagcga gcttcaagcg
     3541 agcgtcgtcg cggggctcga agagcagacc acggccaccg gcgaaatctc gcgcaacctc
     3601 gccgacggcg tgcgcggcgc gagcgagatc acgagcaaca tcggtggcgt agcccaggcc
     3661 gcccgcggga cctccgcggg agccgtggac accaagagcg cggccgagga actctctcga
     3721 atggcggccg agctccgccg agtcttgggt gggttcgcct actgaggtga gctatgcgcg
     3781 cgatcgtagt cgacgactcg aggaccatgc gcacgctcct gaagacggtg ctccgtgagg
     3841 ccggattcga cgtcgtcgcg gaagcggaga acggccgcga ggcccttgaa gcgctcgcgc
     3901 ggactggtcc ggtcgagctg atgttgctcg attggaacat gcccgtgatg aatggatacg
     3961 acctcctgct cgagatccgg agcaatagca agctgagccg aatgcgcacc atgatggtga
     4021 ccaccgagac ggaggcgagc caaattctgc gtgcgctcga ggcgggcgcg gacgagtact
     4081 tgatcaagcc gttcggtcgt cacgcgatcc gagaaaagct cgagctgatg gggcttttcg
     4141 aatgaaggcc gaccgcaaga tccgaattct cgtcgtcgac gactcggcgc tggttcgcaa
     4201 gatcgtgacc gatgccctga acggcgaagg cgacatggag gtggtgggcg tcgcgtccaa
     4261 cggcaagatc gcgctcgaga agctcgagtt cttgaagccg gacgcggtcg tgctcgacct
     4321 cgaaatgccc gaaatggacg ggcttacgac gctcaaacgc ctgcgaaagg actttcgtct
     4381 gcttccggtg gtcgtcttca gcacgctgag cgagcgcggc gcgaagatca ccctggacgc
     4441 gctcacggcc ggtgcaagcg actacctttg caagccgagc gggacgggta gcctggccgc
     4501 gtcgaaggac ttgctgcacg gcgttctcgc gccgaagctc cgcgcgctcg cgcttcgcaa
     4561 cgtcgccgtc gcgccgagga acgcgccggc cgcccccgcg ccgtcgccga tgccggcaat
     4621 ggttcctgcg cgaagggtca acgaaccgat cgaggccatt tgtatcgggt gctcggcggg
     4681 tggtccgcag gcgctggccg aagtgatacc caagctgccg gcgtcgctcg gcgtgccggt
     4741 catggtcgtg cagcacatgc cgccgctgtt cacgaagttc ttggccgagc ggctcgcgca
     4801 gaccgccaag ctgagggtaa tcgaagcggc cgacggcatg agcgtggagg gcggctcggt
     4861 gtatgtcgct cgtggtggag aacacatgcg catcgtgctg cgcggcgcgc gcccggttct
     4921 cgtgctcgac accggcgaat tggagcacgg ctgtcgtccg gcggtggatc cgctgtttcg
     4981 ctccgcggcc gcgctttacg gtcagcattt gctggcggtg gtcatgacgg ggatgggcgt
     5041 ggacggaacc caaggagctc gcgcggtgca aaaggcgggc ggccgcgttt gggcccaaga
     5101 cgagcaaagc tcagcggtat ggggaatggc cgggtcggtc gtgaaggcgg gtgtggtgga
     5161 gcgcatcttc aaattgagtg cgttggccga agacttggcg cgaagcgtgg atgtcagtcg
     5221 acgcccggta gcgggaggcg cacgcggctg acatggcgct gcttccaggt gagctcggcg
     5281 tcgtccagcg gctggtgctg gagcgttccg cgatagtgct cggagacgag caaggctact
     5341 tggtggagtc ccgcctcgcc aatctcgcgc gtgagtccgg ctgtgacgtt cccacgctgg
     5401 tcgcgcggct agggcgcgaa ccgcacggcg gcttgcacga acgcgtcgtc gaagcgatga
     5461 cgacgaacga gacgagcttc tttcgggacc gtcatccctt cgccgccgtt caagagcgcg
     5521 cgctccccga gctcatgttg atgagaggcc gcgagcggaa gctgcgggtg tggtctgcgg
     5581 cctgctcttc aggccaagaa gcgtacagcc tcctcatgtt gatgtgggag gcgttcccga
     5641 tgctcaagct ctgggac
//
LOCUS       DASTKS010001324         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_436297, whole genome shotgun sequence.
ACCESSION   DASTKS010001324 DASTKS010000000
VERSION     DASTKS010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436297"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFQ35_28365"
     CDS             <1..450
                     /locus_tag="VFQ35_28365"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9934665.1"
                     /translation="IDVERFDAIVVAGGQSPMFTFDRAVDLQRKFVAFYESGKLAAAL
                     CHGVALLRYVKLSNGDYLAKGKTVTGFANVEEDFADNAVWSLGALARDQHFMPWRIED
                     ELKRLGANYVQAGMFRGFAVRDGNLITGQQNFSGGETADLVIETLGR"
     gene            579..1214
                     /locus_tag="VFQ35_28370"
     CDS             579..1214
                     /locus_tag="VFQ35_28370"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9934666.1"
                     /translation="MKSILGTEFRRVENREYLGKPAIVGVATRVYDTSVEDMWDAITT
                     PERLVRWFLPVEGDLKVGGRYQLKGNAGGTITRCDPPAALDLTWEFAGATSWVNVRLA
                     AEGAKTRLTLEHIAHKGGIGEEHMKKFGPGATGVGWDLSLHGLKQHLLNPSVALDPAA
                     FEAWSLSSEGKAFIRECGEAWGEAYIAAGADPEEARAQALRTIAFYTGAGV"
     gene            1214..1585
                     /locus_tag="VFQ35_28375"
     CDS             1214..1585
                     /locus_tag="VFQ35_28375"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9934667.1"
                     /translation="MHAFDVLGDPVRRRILELLSEGERASGEVVEVIGKEFGITQSAV
                     SQQLKILRENGFASVRAEGARRLYAVDAGPLAEVDAWLEQFRRFWAAPLDALATEIAR
                     GKRARSNTPTRAPPRRVKASK"
     gene            1669..2460
                     /locus_tag="VFQ35_28380"
     CDS             1669..2460
                     /locus_tag="VFQ35_28380"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9934668.1"
                     /translation="MTPEQQREVWASLDRAQLVDVARKGPEPVFATISGAHLYGFASL
                     DSDVDLRGAFMLPANALLGLFPPDETITIEDKTTIDLDWVAHDVRKFARMMTNHNGYV
                     LEQLFSPLVVITTPAHEELMALGKGCVTKPTVRHYQGFARGRRKRLAEPAPTVKHLLY
                     AYRVLLTGIHLMQTGEVIANIQALNERFRDGAVDELVARKRSGAEKMLLNERDLQEHG
                     ARLDRLERELQAAHDASQLPNEATSARALDAFVVQLRLAGARDAA"
     gene            2411..3634
                     /locus_tag="VFQ35_28385"
     CDS             2411..3634
                     /locus_tag="VFQ35_28385"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9934669.1"
                     /translation="MRSSCSFASQVHETPPDLPPHLGQTSLGGVMFPLESHTIFLALA
                     GSQAHGTAREGSDVDVRGVCVAPLTTRLSLFQHFEQYEGALPDAFAAQVLPRLRAHPT
                     ASRALEVKSECVIFDVAKFVGLCAATNPNALEVLFSDERDWLWSTASWQKLYEHRHQF
                     LTQKLQQTFLGYAMAQLKRIQTHRSWLLNPPAKKPVREDFGLSSQHGTLTSDDQNRIE
                     QSVAEAIRSYGIDTLDMPKATRVALQERLTAFQCDALSASADELEQRLRAVATIALNI
                     PLDVVTTLNAEKRYRAATKHWDSYQNWKSQRNPTRAELERRHGYDTKHGMHLIRLMRM
                     GLEALRHGELHVRRDDSEELSAIRDGALTYEALLNLATELQRAMHEAALTTELPPDID
                     AARVDALLADILLAA"
     gene            complement(3732..4529)
                     /locus_tag="VFQ35_28390"
     CDS             complement(3732..4529)
                     /locus_tag="VFQ35_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9934670.1"
                     /translation="MNTNATGSGPHKVSVETNADSGIKEGTIFRPTDLGGAEKYPIFV
                     WGEGGCSLNGLSNSAAMAEIASHGYFVIADGTPNGSDSRPMDASNPAAMGAPLLAYIS
                     WAIAENEKPCSAYYHALDTSKVAANGFSCGGLMSEGTAGDPRITTWGLNSSGLFGANQ
                     SFYKTVHTPVLIVLGGADDVAYENGERDYDNLAALGIPVMLFSKALGHGGDLFKARGG
                     DFAKIDLAWLNWQLKGDETATGKGLLIGSGCPYCSDSAWETKSKNIQ"
     gene            4522..4788
                     /locus_tag="VFQ35_28395"
     CDS             4522..4788
                     /locus_tag="VFQ35_28395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934671.1"
                     /translation="MFTALDAVQLPLAVPPPLAAPPLLAPPVAARPPPLAPPVAARPP
                     LLVPPVAARPPLLEPPEEDAVDPPEFPEDLPPLALAPPELPPEL"
BASE COUNT          914 a         1625 c         1602 g          872 t
ORIGIN      
        1 atcgacgtcg agcgcttcga cgcgatcgtg gttgcgggcg ggcagtcgcc gatgttcacc
       61 ttcgaccgag ccgtcgattt gcagcgcaag ttcgtagcgt tctacgagtc cggcaagctc
      121 gcagccgcac tctgccacgg cgtcgcactt ctacgctacg tgaagctctc gaacggggac
      181 tacttggcga agggaaagac cgtgacgggg ttcgcgaacg ttgaagaaga ctttgccgac
      241 aacgcggtct ggagcctcgg tgccttagcg cgtgaccagc acttcatgcc gtggcggatc
      301 gaggacgagc tcaagcggct cggcgcaaac tacgtgcaag ccggcatgtt ccgcggcttc
      361 gcggtgcgtg acggcaacct gatcactggc cagcaaaact tctcgggagg tgagaccgcg
      421 gacctcgtca tcgagacact ggggcgctag ggaggccagg cgccgtctcc gcgatgggac
      481 gagcaccgcc ccacgtcgaa actcgcctgg ccaatattag ttatagctaa tattagtgcc
      541 gcctgctagg ctcctctcga cacgctgcag gagccgtaat gaagagcatc ttgggaaccg
      601 aatttcgtcg agtcgagaac cgcgagtatt tagggaagcc cgcgatcgtt ggtgtggcga
      661 cccgcgtcta cgacacgagc gttgaggaca tgtgggacgc gatcacgacg ccagagcggc
      721 tcgtccgctg gtttctcccg gtcgagggag acttgaaggt cgggggccga taccagttga
      781 agggcaacgc cggcggaacc atcacgcgtt gcgatccgcc cgcggcgctg gacttgacgt
      841 gggagtttgc gggcgcaacg agctgggtca acgttcggct cgcggccgaa ggcgcgaaga
      901 cgcggctaac gctcgagcac attgctcaca agggcggcat cggcgaggag cacatgaaga
      961 agttcggtcc cggtgcgacc ggagtcggtt gggatctctc gctgcacggc ttgaaacagc
     1021 atcttctgaa cccgagcgta gcgctcgatc cggccgcttt cgaagcctgg tcgctgtcga
     1081 gcgaaggcaa ggccttcatc cgtgaatgcg gcgaagcctg gggtgaagcg tacatcgccg
     1141 ccggtgccga tccggaggag gcacgggcgc aagcgctgcg aaccatcgcg ttttatacgg
     1201 gcgcaggtgt ttgatgcacg ccttcgacgt cctcggtgac ccagtgaggc gccgcatcct
     1261 cgagctcttg agcgagggag agcgcgcctc gggcgaggtg gtcgaggtca tcggaaaaga
     1321 gttcggcatc acacaatccg cggtgtctca gcagctcaaa atcctgcgcg agaacgggtt
     1381 tgcatcggtg agggccgagg gcgcgcgccg gctctacgcg gtggatgcgg gcccgctcgc
     1441 cgaggtggac gcctggctcg aacaattccg gcgcttctgg gcggcgccac tcgacgcgct
     1501 cgccaccgaa atcgcgcggg ggaagcgcgc gcgaagcaac actccgacac gcgcgccccc
     1561 gcggcgagtc aaagcctcga aataggcact ttggttcgca tcaggtggtc tcaaacgctg
     1621 tggaggtgcg tccggcgcac tcttcggtca agattgcgcc gcggagacat gacaccggaa
     1681 caacaacgcg aggtttgggc atcgctcgac cgtgctcaat tggtcgacgt cgctcgcaaa
     1741 ggccccgaac cggtctttgc cacgatcagc ggggcgcatc tttatgggtt cgcttcgctc
     1801 gacagtgacg tcgatctgcg cggcgcgttc atgctcccgg ccaacgcgct gctcgggctc
     1861 ttcccgccgg atgagacgat cacgatcgaa gacaagacga ccatcgatct cgattgggtc
     1921 gctcacgacg ttcgcaagtt cgcgcggatg atgacgaacc acaacggata cgtgttggag
     1981 caactctttt cgccgttggt cgtgatcacg accccggccc acgaggagtt gatggcgctc
     2041 ggcaaaggat gtgtgaccaa gcccaccgtg cggcactatc aaggttttgc gcgcgggcgc
     2101 cgcaaacgcc tggccgaacc tgctcccacc gtaaagcacc tcttgtatgc gtaccgagtg
     2161 ctgctcaccg gcatccatct gatgcaaacc ggcgaggtca tcgcgaacat tcaggccctc
     2221 aacgaacggt ttcgagacgg agcggtcgac gagctcgtcg cacgtaagcg ctctggagcc
     2281 gaaaagatgc tgctgaacga gcgcgacctc caagagcacg gcgcacggct cgatcgcctc
     2341 gagcgagagc tgcaggcggc gcacgacgcg agccagctac cgaacgaggc gacttcggcc
     2401 cgcgccctgg atgcgttcgt cgtgcagctt cgcctcgcag gtgcacgaga cgccgcctga
     2461 cctaccgccg catctcggcc aaacgagcct cggaggcgtg atgttcccgc tcgagtcaca
     2521 cacgatcttt cttgcgctgg ccgggagcca agcccacggc acggcgcggg agggctcgga
     2581 cgtcgacgtg cgcggggttt gcgttgcccc gctcacgacg aggctgtctc tctttcaaca
     2641 cttcgagcag tacgaagggg cgttaccgga cgctttcgcg gcccaggtgc tgccgcgcct
     2701 gcgagcgcac cccaccgctt cacgcgcgct cgaagtcaaa agcgaatgcg tcatcttcga
     2761 cgtagcgaag ttcgttgggc tctgcgctgc caccaacccg aacgcgctcg aagtgctgtt
     2821 ctcggacgaa cgggattggc tctggtcgac cgctagctgg caaaagctct atgaacatcg
     2881 ccaccagttc ctgacccaaa agcttcagca gacgttcctt ggctacgcga tggcccagct
     2941 aaagcggatt cagacgcacc gctcgtggct tttgaacccg cccgcgaaga agccggtgcg
     3001 ggaagacttc gggctttcgt cgcagcacgg cacgctgacc agcgacgacc aaaatcgcat
     3061 cgagcagagc gtcgccgagg cgatccgcag ctacggaatc gacaccctcg acatgccgaa
     3121 agcgactcgt gtagcgctgc aggagcgcct gaccgcgttt cagtgcgacg cgctgtctgc
     3181 ctccgccgac gagctcgagc aacgcttgcg cgccgtcgcc acgatcgccc tgaacattcc
     3241 gctcgacgtg gtcacgacgt tgaacgccga gaagagatat cgggccgcga cgaagcactg
     3301 ggactcgtac caaaactgga aaagccagcg caatcccact cgcgcggagc tcgagcgccg
     3361 gcacggctac gacacgaagc acggcatgca tctcattcgc ctgatgcgca tggggctcga
     3421 agcgttgagg cacggcgaac tccacgtgcg gcgcgacgac agcgaagagc tctcggcgat
     3481 ccgcgacggt gccctcacct acgaggcgct cctcaatttg gcaaccgagc tccagcgcgc
     3541 catgcacgag gcagccctga cgaccgagct accaccagac atcgacgcgg cgcgcgtcga
     3601 cgcacttttg gcagacatcc tcctggccgc ctgatcgcct gggtaggcgc gccaggaagc
     3661 ttcgctactg gatgttcttc gactgggttc ccaagcgctg tcgcctggga aagcgcgcca
     3721 ggaagccttc gctactggat gttcttggac ttggtttccc aggcgctgtc gctgcaatag
     3781 gggcagccgg agccgatgag caggcctttt ccggtcgcgg tctcgtcgcc tttcagctgc
     3841 caattgagcc acgcgaggtc gatcttggcg aaatcgccgc cgcgcgcttt gaacagatcg
     3901 ccgccgtgac ccagcgcctt gctgaagagc atgacgggga tgccgagggc ggcgaggttg
     3961 tcgtagtcgc gttcgccgtt ttcgtaagcg acgtcgtcgg cgccaccgag tacgatcaag
     4021 actggcgtgt gcaccgtctt gtaaaaggat tggttcgccc cgaagagccc gctgctgttc
     4081 agaccccacg tcgtgatgcg cggatctccg gcggtgccct cggacatcaa accgccgcac
     4141 gagaagccgt tggcggcgac cttgctggta tcgagcgcgt ggtagtaggc gctacagggt
     4201 ttctcgtttt cggcgatggc ccagctgatg taggcgagca gaggtgcgcc catcgcggcc
     4261 gggttcgaag cgtccatggg gcgcgagtcc gaaccgttcg gagtgccgtc agcgatgacg
     4321 aagtaaccgt gcgatgcgat ctccgccatc gcggccgagt tcgacaaacc gttgagtgag
     4381 cagccgccct ctccccacac gaaaatgggg tacttttccg cgccgccgag gtcggtgggg
     4441 cgaaagatgg ttccctcttt gatgcccgaa tcggcgttgg tctcgacgct gaccttgtgg
     4501 gggccactgc cagtcgcgtt cgtgttcacc gccttggacg cggtgcagct gccactcgcc
     4561 gtgccgccac cgctcgcggc tcctccactg cttgcgccac cagtcgccgc gcgccctccg
     4621 ccgctcgccc caccggtcgc agcgcgccct ccgctgcttg tgccgccagt cgccgcgcgc
     4681 cctccgctac tcgaaccgcc cgaagaggat gcggtcgatc cgccagagtt tcccgaagac
     4741 ctcccgccgt tggcgctcgc accgcccgag ctcccgcccg aactttgagc gccacccgac
     4801 gccgaggtgc cgccgctggc accgcccgag ccgtttccgg aggcgttcgt ggaaccccca
     4861 gtggcgctcg cgccgccgaa agcaccgccg gctccggtcg tcccaccgct gccctccgag
     4921 ccgggctcag aactgccaca gccggcgagg aatgcgatgc ttgcaacgag agtcgttatc
     4981 gacgggtgct tcggtctcat gaatgcaact cct
//
LOCUS       DASTKS010001325         5469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_2003316, whole genome shotgun sequence.
ACCESSION   DASTKS010001325 DASTKS010000000
VERSION     DASTKS010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003316"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFQ35_28400"
     CDS             complement(<1..196)
                     /locus_tag="VFQ35_28400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934672.1"
                     /translation="MAVVSSLHESARASANLGGTVLSDRYRVTESLRSSAMSHVYVAE
                     DLVKHKRVAIKVLRDELCDER"
     gene            325..987
                     /locus_tag="VFQ35_28405"
     CDS             325..987
                     /locus_tag="VFQ35_28405"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9934673.1"
                     /translation="MNTPDPDDPESEPALTTTSSASIRELILGSWQLAIVVVDVSRRY
                     RYANSHGKEILERGNVFKLRAGSLVCANPELDAELAERVTAAARKTGLGHEGIIGWSV
                     GRGADPEDRVIVRFSALTSPKRSTPLIELHVARAEADFVPPQALVAHSLGLTRMQARV
                     AVEIARGNATGKIAEQLGIRPDTVKDHLKCVYQRLHIDGARERGMDAKAILIRKIMAL
                     SY"
     gene            1042..3585
                     /locus_tag="VFQ35_28410"
     CDS             1042..3585
                     /locus_tag="VFQ35_28410"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934674.1"
                     /translation="MLRKTCQAIWLTKAASYVLLGCASTGAPLPRTAPLPRAATELKT
                     MRPAESAIHFYPTAPEIQLRLAQRFASGDGVPQDAFRASNWAERSALAGNSAALAWLE
                     QRANQKDPFSALALANIYSTAASPDKAASHLAAAKTGLARLVQKADHTAEYALALGEL
                     GTTRSAPLRLGCFSIECVRVVLSGAEAKDAQALFERAAHGGYAPAQIAFGLMLANTLG
                     LSNQTARGSIESAARQQDAQAELLLGNMGVWAHDEAAAQGRYLRAAALGLPEAEGIVG
                     KRLLDGVGAEANQAQGRDWLRRASEHGDPNAMLELSRLYAEGTRIAKDQELSVSLCRR
                     AAERGLAAAETALGVHYREGSGVARDPIEARKWFEKAASHGDVDGEFYLALSFDTDDA
                     NANPKRALELYERAAAHGSVAAERNIGLLYQSEQLGKDYARALTWLQRAAEHGSDAAR
                     VQIGLMYDGGKGVAKDSKQALRWFEQAAENGDARALVLAGLHYQEADGVERNTERAKD
                     YYTRAADKGNALGIVHLGILYELGIGVERDPQRALGWYRKAATSEDGALGASYLGHAL
                     RFWDGPDHDATEGCRLLLVAAKEGYPRAQFDIGLCLESGVGVPKDENAAAYWYLEAAK
                     QNYSDAEYELALMCLDGRGVARDRTAAEKWLERAISHGNQAAVGELSNLRERGNCDRS
                     AKTELFGTKVSCASRRELRRALKRGNASGVSEDDNRWYDSYESSALLEESQSLELWYT
                     RDNGWLARARYEFPSWLDTEQVARVAAMVTRKYGAPRARNGLVAVGPVRYEWALPDGV
                     RLVVRRGWPGTTTYLEYENPKRLSQMEREIAADERAEKVRQAARQSTAF"
     gene            3588..4886
                     /locus_tag="VFQ35_28415"
     CDS             3588..4886
                     /locus_tag="VFQ35_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934675.1"
                     /translation="MRTGLVSILVAAISSMPGCVSVHESAPVTSERLLRTSQAERRVT
                     GEEYPTRLLGGSGLSADGRKLALELLIQHTPRCEVVRTKTWETTTTTERTSRAGVALT
                     AGALLSSLFVVGVIGAAQGESLSKKSDSGRTELTSTGVEVYTGVWGVPLLIDGVIASI
                     GSIDSVDSRTEDRVERHVRPCAGDAAPGADLSVTLTADGKPISGRRTGPDGKVKFEVA
                     REELASPPKELKVFVGELPTLPSADLVALLEHVTENWSTGELPPVPSVTPTPAAPPVG
                     PSLFGVALSTATRELFESAVISHGCSRTKVENRNVSRYTLECLALPGFREMKVLYDGS
                     DHVVRVKYSGALDSSQCATMRRLLTEEYGSPRSETGRQSDWRLPAEFSLTLDCRKSPA
                     ELLYNNIALDTELSHAAEQQQEQRERERLDELKKQRGKAF"
     gene            4927..>5469
                     /locus_tag="VFQ35_28420"
     CDS             4927..>5469
                     /locus_tag="VFQ35_28420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934676.1"
                     /translation="MKTNPFSKQTLSRLARTTILVACLAPAACKGRSAGNTTATVNAL
                     VTREPRETKFDNGQVKETFSVTKDKSGNYVKDGSYARFHQSGQKAEEGTYKADKIDGH
                     WQEWDEQGKLTLDLHFADGKREGAYAKYRNGSPRIVGNYSQDKLSGPFKFIDDGSVIT
                     GEMARGVPEGPWTLTDASGKL"
BASE COUNT         1119 a         1732 c         1771 g          847 t
ORIGIN      
        1 agcgttcgtc gcagagctcg tcgcgaagca ccttgatcgc aacgcgcttg tgcttgacca
       61 gatcttccgc cacgtagacg tggctcatgg cgctcgagcg caacgactcc gtcactcggt
      121 agcgatcgga gagtactgtt cccccgaggt ttgcgctggc gcgcgctgac tcgtgcaggc
      181 tagaaacaac cgccatcgga acgtcgtgct cggaggcccc ccgagtgggg gaagcatcta
      241 accaggcgcc atggtatccg agttgtcgtt gaagttgtag ccggtccggc gtcgagcccc
      301 gccagtccga gggaaggacc aaccgtgaat acacccgatc ccgacgaccc cgagagcgag
      361 cccgcgctca cgaccacctc ttcggcgagc attcgcgagc tgatactcgg ctcttggcag
      421 ctcgcgattg tggtcgtgga tgtctcacgc cggtacaggt acgcaaactc ccacgggaag
      481 gaaattctcg agcgaggtaa cgttttcaag ttacgcgctg ggagcttggt ctgcgcaaac
      541 cccgaactcg acgccgaact cgccgagcgc gtcaccgctg cggcgcgcaa aacagggctc
      601 ggacacgaag gcatcatcgg atggtcggtc ggccgcggcg cagatccaga ggaccgagtc
      661 atcgtgcggt tcagcgcgct cacgagcccg aaacgctcga cgccgctgat cgaactgcat
      721 gtcgcgcgag ccgaggcaga cttcgttccg ccccaggcat tggttgctca ctcactcggc
      781 ctcacccgaa tgcaggcaag ggtcgccgtc gaaattgctc gggggaacgc gacggggaag
      841 atcgccgagc agctcggaat ccgacccgac accgtaaaag accacttgaa atgcgtctac
      901 cagcgcttgc atatcgacgg cgcgcgcgaa cgcgggatgg acgccaaggc gatcctcatc
      961 cgaaagatta tggccttgag ctattgaccc cccaagtggg ggaagcgcgc tcggggaaat
     1021 gggtatagct ccgaagaagt catgctgaga aagacgtgcc aggcaatttg gctgacgaaa
     1081 gcagcgtcct acgtgctgct cggttgcgcc tcgaccggag caccgctgcc gcgcacggcg
     1141 ccgctacccc gcgccgcgac cgaactcaag actatgcgcc ccgcggagag cgcgatccat
     1201 ttctacccga ctgctccgga gattcagttg cggctagcgc agcgattcgc gagcggtgac
     1261 ggcgtacccc aagacgcttt ccgcgcgagc aactgggccg agcgctcagc tctcgcgggc
     1321 aactcggctg cgctcgcctg gctcgagcag cgagccaacc agaaggatcc attttccgcg
     1381 ctcgccctcg cgaacatcta ctcaactgct gcttcgcccg acaaggcggc ctctcacctc
     1441 gccgcggcca agaccggcct cgcgcgtcta gtccagaagg cggatcatac tgccgagtac
     1501 gcgctagccc tgggggaact cgggacgaca cggagcgcac cgctgcgcct cggctgcttc
     1561 agcatcgagt gcgtccgcgt cgtgctgagc ggcgccgagg cgaaggacgc gcaagccctc
     1621 ttcgagcgtg ctgcccacgg gggatacgcg cccgctcaaa ttgcgtttgg cctcatgctc
     1681 gccaacacat tgggcctctc gaaccaaacg gcgagaggct ccatcgagag tgccgcacga
     1741 caacaggatg cccaagcgga actcctgctc ggtaacatgg gggtttgggc ccacgacgag
     1801 gccgccgctc aaggacgcta cctgcgcgcc gccgcgttgg ggttgcccga ggcggaaggg
     1861 attgtcggga aacggctctt ggacggagtc ggtgccgaag ccaatcaggc gcagggacgc
     1921 gactggctcc gacgtgcgag cgagcacggc gacccgaacg cgatgctcga gctgagtcgc
     1981 ctctacgcgg agggcactcg catcgcgaag gaccaagagc tcagcgtgag cctttgccga
     2041 cgcgcggccg agcgggggct cgcggcggcc gagaccgcgc tcggtgtgca ctaccgagaa
     2101 ggctcgggtg tcgctcgcga tccgatcgaa gctcgcaaat ggttcgaaaa agccgcttct
     2161 cacggcgacg tggacggcga attctatcta gcgctctcgt tcgacaccga cgacgccaac
     2221 gcgaacccga agcgagcgct cgagctctac gagcgcgcgg cagcgcacgg cagcgtcgcg
     2281 gccgagagga acatcgggct tctctatcag agcgagcagc tcggtaagga ctacgcgcgg
     2341 gctctcacct ggcttcaaag ggcagcggag cacgggagcg acgccgctcg agttcaaatt
     2401 ggtttgatgt acgacggagg taagggcgta gcgaaagact cgaagcaagc cctgcgctgg
     2461 ttcgagcagg ccgccgaaaa cggggacgcc cgcgcgttgg tcttggctgg gctccactac
     2521 caagaggccg acggtgtaga gcgcaacaca gagcgcgcaa aggactacta cacccgcgca
     2581 gcagacaagg gcaatgcgct agggatagtg cacctcggta tcctctacga actcgggatc
     2641 ggcgtcgagc gcgacccgca acgagcgctc gggtggtatc gaaaggccgc aacctccgag
     2701 gacggcgcgc tcggagcctc ttacctcggt cacgcgttac gcttctggga cggccccgac
     2761 cacgacgcga cagaaggttg ccgcctcctg ctcgtcgcgg cgaaagaggg ctatccgcgc
     2821 gcccaattcg acatcgggct ttgcctcgaa agcggggtcg gggtgcccaa agacgagaat
     2881 gccgcggcct actggtactt ggaagcggcc aagcaaaact actcggacgc ggagtacgag
     2941 ctcgcgctca tgtgtctgga tgggcggggc gtcgcgcggg atcggaccgc ggccgaaaaa
     3001 tggctcgaac gagcgataag ccacggaaac caggctgcgg tcggagagct ctcgaacctg
     3061 cgcgagcgcg gcaactgtga tcgcagcgcc aagaccgagc tcttcgggac gaaggtatcc
     3121 tgcgcgagcc ggcgcgaact tcgccgagca ctgaagcgag gcaacgcgag tggcgtgagc
     3181 gaagacgaca atcgctggta cgattcctac gagagctcgg ccctcctcga agaatcgcaa
     3241 tcgctcgaac tctggtacac acgcgacaat ggctggctcg cacgcgcccg ctacgagttc
     3301 ccctcctggc tcgacaccga gcaggtagcg cgggtcgcgg ccatggtcac gcggaaatac
     3361 ggagctccgc gcgcgcgcaa cggcctcgtc gccgtcggcc ccgtacgcta cgagtgggcg
     3421 ctgccggacg gcgttcgctt ggtggtgcgc cgcggttggc ccggaactac gacttacctc
     3481 gagtacgaga atccgaaacg gctgtctcaa atggaacgtg agatcgctgc agacgagcgc
     3541 gcggagaagg tccgacaggc ggcgcgccag tcaaccgcgt tttgaccatg cgtaccggtc
     3601 tcgtctccat tctcgtagcc gccatttcca gcatgccggg gtgtgtttcc gtccacgaga
     3661 gcgcccccgt aacgagcgag cggctgctgc gaacgagcca agccgagcgc cgcgtcactg
     3721 gcgaagaata cccgacgcgc cttctcggcg ggagcgggct gtcagcagac gggcggaagc
     3781 tcgccctcga gctcctgatc cagcataccc ctcgctgcga agtggttcgc accaaaactt
     3841 gggaaaccac gaccaccacc gagcgcacct cgcgagcggg tgtcgcgctg actgccggcg
     3901 cgctcttgag ctctttgttc gtcgtcggcg tcattggcgc tgcccagggg gagtcgctct
     3961 ccaagaagag cgacagcggg cgaaccgaac tcacgtcgac cggagtcgag gtgtacacgg
     4021 gagtatgggg agtgcccctt ttgatcgacg gtgtcatcgc gagcatcggc tcgatcgatt
     4081 cggtcgactc tagaacggag gaccgcgtcg agagacatgt gcgaccttgc gccggcgacg
     4141 ccgcgccagg cgctgaccta tcggttacgc tcaccgccga cggcaagccg atttcagggc
     4201 ggcgaacggg tcccgacggc aaggtgaagt tcgaggtggc gcgcgaggaa ctcgcttcgc
     4261 cgcccaaaga gctgaaggtc ttcgtgggcg agctgccgac gcttccttcc gcggacctcg
     4321 tcgcgctgct cgaacacgtc accgagaatt ggagcaccgg cgagctgcca ccggtgccga
     4381 gcgtgacgcc gacaccagcg gcgccgccag ttggtcctag cctgttcggg gtggcgctct
     4441 cgacggctac tcgagagctc ttcgagtcgg ccgtgatttc ccacggctgt tctcgcacga
     4501 aggtcgagaa ccgcaatgtc agccgctaca ctctcgaatg cctcgctctc cccggattcc
     4561 gagagatgaa ggtgctctac gatggcagcg accacgtcgt acgcgtcaag tattcgggcg
     4621 cgctcgactc atcacagtgc gcgacgatgc ggcgcttgct gaccgaagag tatgggagcc
     4681 cacgaagcga gacggggagg cagagcgact ggcgccttcc agcagagttc tcgctcacgc
     4741 tcgattgtcg caaatcgccg gcagagctcc tctacaacaa catcgctttg gacacagagc
     4801 tcagccacgc ggctgagcaa cagcaggaac agcgagaacg agaaaggctc gacgagctca
     4861 aaaagcagcg aggaaaggcg ttctgagggt cgggccgaga accttgtgag ggcgggaaat
     4921 acaacaatga aaacaaaccc attttcgaag caaacactct ctcgcctggc gcgaacgaca
     4981 atcctcgtcg cgtgtttggc gccggctgca tgcaaaggcc gctccgccgg caatacgacc
     5041 gcgaccgtca acgcgctcgt cacgcgcgag ccgcgtgaaa ccaaattcga caacggtcag
     5101 gtcaaagaga ccttctcggt caccaaggac aagagcggca attacgtgaa ggacggcagc
     5161 tacgcgcggt tccatcagag cggtcaaaaa gcagaggaag ggacctataa agccgacaag
     5221 atcgacggac actggcagga gtgggacgag caaggcaagc tgacgttgga cctgcacttc
     5281 gccgatggaa aacgcgaggg cgcttacgcg aagtaccgga atggctcgcc acgaatcgtt
     5341 ggcaactact cgcaagacaa gctgagcggc ccgttcaagt tcatcgacga cgggagtgtc
     5401 atcaccgggg agatggcgcg aggcgtaccc gaggggcctt ggacactcac ggacgcctcg
     5461 ggcaagctg
//
LOCUS       DASTKS010001326         6411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_436787, whole genome shotgun sequence.
ACCESSION   DASTKS010001326 DASTKS010000000
VERSION     DASTKS010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436787"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFQ35_28425"
     CDS             <1..833
                     /locus_tag="VFQ35_28425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934677.1"
                     /translation="KAALALDQRSFSRRVLQPIVRPIARAFLIVVAAFRLVVPQALTS
                     SRLLHYLIYIGLKYFVTPQASFLILRHFHLGSNVLRFLASNSGEEVPTEPLRPLRVED
                     VRDHLFLKHDLNLYNFIIALNTRLAEKQRGLVPAQPVDFTPIDERPIQFAPFRRGFTN
                     VLDCTTAIELYTPLYHLLLGERDFSRAVLSLQLDETLAVYAARVLGDGTHLAIVNNRH
                     PLIPLTSLRAGFRLVLHGLATELLHAVLVEHKLNQRTGASPADHGLRSLFMDRARVPS
                     "
     gene            857..1612
                     /locus_tag="VFQ35_28430"
     CDS             857..1612
                     /locus_tag="VFQ35_28430"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9934678.1"
                     /translation="MPTRLDELSYAFVYVFTVIGFVFIYFTLGFSSEYLVTRVLPARG
                     QGAALSQRALAPGQKRSEIVSSLRSISLFGGYGVFTLFGWRHGLWGIAPATPVRVWLE
                     LVLLVLWNELHFYVIHRALHVGPLFRWVHREHHRAIRPTSWSTFAMHPLEAILLGSVM
                     VTAMPFHAFSWLTLLSFPLVSLALNNIGHMNYDVLPGASDWHPLSGSRRHELHHRRVH
                     GNYGFMLPLLDRLFRTELTTSPPPTPPSSLPPH"
     gene            complement(1788..2720)
                     /locus_tag="VFQ35_28435"
     CDS             complement(1788..2720)
                     /locus_tag="VFQ35_28435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934679.1"
                     /translation="MKRRDQARFEAELLQTLRCEMEGPPRAAARVQVSKQLEATFAGL
                     DLGLANLASLPHSATPLKAGVTKLGSSAASAKASSAAAVKFGAPMGAAKGFLGGILPA
                     LSATAHPVATVLATFALGAAAGTGIMVASEATQAPHKPNVVAVRPPPEKSPPKPPPEA
                     APPLNAHAPHALLNPTDDARIVGHAVAPVSPRGRDAKADPPRRLAAATPPVASQAAPE
                     LGPLAAQQTLLDQARKALSRGDAESALSSLAEHERAFNATLFEEERLSLKIKSLAASG
                     RRDDARAMADAFGARFPRSILLPSVKAAASGEKP"
     gene            complement(2717..3316)
                     /locus_tag="VFQ35_28440"
     CDS             complement(2717..3316)
                     /locus_tag="VFQ35_28440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9934680.1"
                     /translation="MVRFLRAQGANVQPDQSRARSRASQPEAAGRADFRDVFQLEFDY
                     VWHTLRRLGVPERDLADVTHDVFERVYLRLDRYDKSRPLRPWLFGFAFRVAADYRRLS
                     RNRCEQLGLADEPHDPRLSPAEELVRAERVTLALAALDSIEFERRAVFVLHELDDYPI
                     PEVAEALGINVNTAYSRLRLAREEFRRAAKRLRAQQEAT"
     gene            complement(3344..4426)
                     /locus_tag="VFQ35_28445"
     CDS             complement(3344..4426)
                     /locus_tag="VFQ35_28445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934681.1"
                     /translation="MQSVRGVAVLAACLFGTVAKRANATPRADFRYLRDEGALSCPSE
                     ATLRAAVAARLGYDPFEKGAPRIVRATIETEGEGYTARVELQGGRDGQMVREFSTHGS
                     CNDLVAAIALSISLAIDAEHSATEPPSSSAEFPTAAPPNVSKNGEPASATQAPKAVVV
                     ASQTLTPAAPGPRAELRCPICETKVVERSRSPGNSRLPFDFVAGAHAELAFGTAPATA
                     VGASIFIQGRRKALSLGLELRVDAPAGMDLPSGGHVQSGLVGFSLVPCLHFQWFRGCA
                     VAFAGEIWGRSQNIDVPRTAIAFHGAVGLRAGFEWPRNGAWALSAHTDALALLSPVDI
                     TINGSTVVWSAPSVFATVGAGALLRF"
     gene            4577..6040
                     /locus_tag="VFQ35_28450"
     CDS             4577..6040
                     /locus_tag="VFQ35_28450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934682.1"
                     /translation="MLEPGELIDGKYLVQRLIGRGGMGAVYEGRHTLIERRVAIKVVF
                     DDVGEAGASRFEREVRAAGRIGNDHILEIIDVGTLPSGSRYMVSEFLDGEPLSGRLQR
                     LGPMDPRTIGGLMVQLLTGLGAAHHVGILHRDLKPDNIFLVREKAGKTDFVKIIDFGI
                     SKFQSLSGSSDSMKMTATGVVVGTPYYLSPEQARGVRDIDARSDLYAVGVIMYECATG
                     RVPFQADSFNQLIFKIALEQPPPLESIVPGIDPWFVAIVQRAMARDPAQRFQSAYEMR
                     DEIARWLGMSQSSGARAAAPGFDATVMAEANFTPVLQPRVPTPSSFGRTSEPLTDELL
                     APRRSGSAKVLLLAAPLVLVIAGAVFFIVSHQNGGEPTASSAPSATPPAASTAAVALK
                     PPPEPSATVDVAPPRVEVETPEVPLSALPPLEAVRPNSAKRFPAQRPTSRPSPAGHAR
                     ADTAPPSAVQRPEPEPPRPTPAQRPSTRPNSDRDFGY"
BASE COUNT         1003 a         2174 c         2079 g         1155 t
ORIGIN      
        1 ccaaagccgc cctcgccctc gaccagcgct cgttctcgcg gcgagtgctg cagcccatcg
       61 tgcgcccgat cgcgcgggcg tttctgatcg tggtggcggc gttccgcttg gtcgtcccgc
      121 aggcgctgac gtcttcgcgg ctcttgcact acctcatcta catcggcctc aagtacttcg
      181 tcacgccgca agcaagcttc ttgatcttgc gccactttca cctcggctcg aacgtgctgc
      241 gctttctggc gtcgaactcc ggcgaagagg tcccgaccga gccgctccgg ccgctacgcg
      301 tcgaggacgt gcgcgaccac ctttttctaa agcacgatct caacctctac aatttcatca
      361 tcgcgctcaa tacgcggctc gccgagaagc aacgcgggct cgtgccggcg cagccggtcg
      421 acttcacgcc gatcgacgag cgcccgattc agttcgcccc gtttcggcgc ggcttcacca
      481 acgtgctcga ctgcacgacc gcgatcgagc tctacacgcc gctctatcac ctgctgctcg
      541 gcgagcgcga cttctctcgc gccgtgctct ccctgcagct cgacgagacg ctcgcggtct
      601 acgcagcgcg cgtgctcgga gacgggacgc acctcgccat cgtgaataat cgacacccgt
      661 tgatcccgct caccagcttg cgggctggct ttcgtctcgt gcttcacggc ttggcgaccg
      721 agctgctcca cgcggttctc gtcgagcaca agctcaatca gcgaactggc gcgagccctg
      781 ccgatcacgg cctgcgcagt ttgttcatgg accgcgctcg ggtgccgtcg tgagcaccta
      841 ctctcgcgag tgagcgatgc cgacgagact cgacgagctc tcctatgcgt tcgtttacgt
      901 ctttacggtc ataggtttcg ttttcatcta cttcacgctc gggttcagct cggagtatct
      961 cgtcacgcgg gtcttgccgg cgcgcgggca aggcgctgcg ttgagccagc gcgccctcgc
     1021 tcccgggcag aaacggtccg agatcgtgag ctcgcttcgc tcgatatcgc tctttggtgg
     1081 ctacggcgtg ttcacgctct tcggctggcg tcacggcctt tgggggatcg ccccggcgac
     1141 cccagtgcgt gtctggctcg agctcgttct gctggtgctc tggaacgaac ttcactttta
     1201 cgtgattcac cgggcgctcc acgtgggccc gctgtttcgc tgggttcatc gtgagcacca
     1261 ccgcgcgatt cgcccgacgt cgtggtccac ctttgcaatg caccccttgg aggcgatttt
     1321 gctcggcagc gtgatggtta cggcgatgcc attccacgcc ttttcttggc tcacgttgct
     1381 gtcgttcccc ctcgtgagcc tcgcgctcaa caacatcgga cacatgaact acgacgtgct
     1441 gcccggcgcg tccgactggc atccgctctc cggcagtagg cggcacgagc ttcatcaccg
     1501 ccgcgtccac gggaactacg gcttcatgct accgctgctc gaccggctct ttcgaaccga
     1561 gctgacgacg tcgccgcccc cgactccgcc aagctcgctc ccccctcatt gaggaaacgg
     1621 gcttccgctc tcccctcact ccgcgacccg gcttccagcg gcgccctcgg gccgccgaag
     1681 ccagcgcgat cccggggaga gcggatcgcc aatttcagtt tcgatttttc ctaccgcacg
     1741 aggcttgccc tgcctgccgt tggtttgggg cggggcttac ctatccgtca cggtttttca
     1801 cccgatgctg ctgctttgac cgaaggcagc agaatactgc gcggaaagcg ggcgccgaac
     1861 gcgtccgcca tggcgcgcgc gtcgtcgcgc cgaccgctcg ccgcgagcga cttgatcttc
     1921 aaagaaagcc gctcttcttc gaacaatgtc gcgttgaagg cgcgctcgtg ttcggcgagg
     1981 gaactgagcg cgctttcagc gtcgccgcgt gagagcgctt tgcgcgcctg gtcgagcagg
     2041 gtctgttggg ccgcgagcgg gcccaattcg ggagcggcct gtgaggcgac tggcggcgtt
     2101 gcagcggcca gtcgtcgcgg cgggtctgcc ttcgcatcgc gaccacgcgg agacacgggc
     2161 gcaaccgcgt ggcccacgat gcgagcatcg tcggttgggt tgagcagagc gtgaggggcg
     2221 tgcgcgttga gggggggcgc ggcttcgggt ggcggctttg gcggactctt ttccggcggc
     2281 gggcgcacgg ccacgacatt gggcttgtgc ggagcttggg tcgcctcgct cgctaccatg
     2341 atgccggttc cagccgcggc cccgagggcg aacgtagcca acaccgtcgc taccggatgt
     2401 gcggtcgcgc tgagcgcagg caagatgccg ccgagaaagc ctttggccgc gcccatcggt
     2461 gcgccgaact tcactgccgc cgcggaggag gccttcgcgc tcgccgcaga cgaaccgagc
     2521 ttcgtcacgc ccgccttcag cggtgtcgcg ctgtgcggca gcgacgcgag attcgcgagg
     2581 ccgagatcga gccccgcgaa cgtagcctcc agctgcttcg acacctgcac gcgcgccgcc
     2641 gcgcggggag gcccctccat ctcgcaacgc agcgtttgca ggagctcggc ctcgaagcgg
     2701 gcttggtcgc gcctcttcac gtggcctcct gctgcgctcg aagccgcttg gccgcgcgcc
     2761 ggaactcttc gcgcgcgagg cgcagccgcg agtacgcagt gttcacgtta atgccgagcg
     2821 cctcggcgac ctccggaatg ggatagtcgt cgagctcgtg gagcacgaac acggcgcggc
     2881 gttcgaattc gatcgaatcg agcgcggcga gcgcgagggt tacgcgttcg gcgcgcacca
     2941 actcttcggc aggagaaagg cgcgggtcgt gcggctcgtc agctagcccg agttgctcgc
     3001 agcggttacg cgacaagcgc cgatagtctg ctgcgactcg aaaagcgaag ccgaacaacc
     3061 acggacgtag cggtcgcgat ttgtcgtaac gatcgaggcg gaggtacacg cgctcgaaga
     3121 cgtcgtgcgt cacgtcggcc agatcacgct cggggacgcc caggcgccgc agcgtgtgcc
     3181 acacgtagtc gaactccaac tggaagacgt cgcggaagtc cgcccgcccc gccgcttcgg
     3241 gctgcgaggc gcggctccgc gcacggctct ggtcgggctg cacgttggct ccttgggccc
     3301 gcaggaatcg aaccatcacg aaaaaccgat cgtttgtgct ttctcagaat cggagcagcg
     3361 ccccggctcc gacggtagcg aatacgctcg gcgcgctcca gaccacagtc gagccgttga
     3421 tcgtgatgtc gaccggcgag agcagcgcga gcgcgtcggt gtgcgcggac aacgcccagg
     3481 caccattccg tggccactcg aatccggcac gaaggccgac agcgccgtgg aacgcgattg
     3541 cggtgcgagg cacgtcgatg ttctgcgagc gaccccaaat ttcgccggcg aaggcgaccg
     3601 cgcacccgcg aaaccactgg aaatgcaggc atggaaccag cgaaaagcca accagcccgc
     3661 tctgcacgtg ccctccggag gggagatcca tcccggcggg tgcgtcgacg cgcagctcca
     3721 agccgagcga aagcgctttg cgccggccct gaatgaagat gctcgccccc accgcggtgg
     3781 ccggagccgt cccaaaagcg agctccgcgt gggcgccagc cacgaaatcg aagggaagac
     3841 gcgaattgcc gggcgatcga ctgcgctcga ccactttcgt ttcgcaaatc ggacagcgca
     3901 gctccgctcg cggaccaggc gccgccggag tgagcgtctg cgaggcgacc accaccgctt
     3961 tcggcgcttg cgtcgcgctc gctggctcgc cgtttttcga aacgttcggt ggcgccgcgg
     4021 ttgggaactc cgcgctcgac gagggcggct cggtggccga gtgttcggcg tcgatggcga
     4081 ggctgatcga gagcgcgatc gcggcaacta gatcgttgca gctgccgtgc gtcgagaact
     4141 ctcgcaccat ttgcccgtcg cgtccgcctt gcagctcgac gcgggcggtg tagccctccc
     4201 cttcggtctc gattgttgcg cggacgattc gcggcgcgcc cttttcgaac ggatcgtacc
     4261 cgagccgcgc agcgacggcg gcacgtagag tggcctcgct cgggcaggaa agagcccctt
     4321 cgtcgcgcag atagcgaaag tcggcgcgag gagtggcgtt agcccgtttg gcgaccgttc
     4381 cgaagaggca ggccgcgagc acggctactc cccgaaccga ttgcacccgg aaccgctcgc
     4441 tcaattgcct ccaaagagcg cttatctccg ggtacttgcg acagcaagcg aacccgttcg
     4501 gtcgccgggc ccaaggtagc caggcaggca aatgccattc gacagttggg gtcagatgct
     4561 agaatcgcga ggccgaatgc tggagccggg tgagctcata gacgggaagt atttggtcca
     4621 gcggctgatc ggtcggggtg gcatgggggc ggtctacgaa ggccgccaca cgctgatcga
     4681 gcgccgcgtg gcgatcaagg tcgtgttcga cgacgtgggc gaggcgggag cctcacgttt
     4741 cgagcgcgag gtgcgcgccg cgggtcggat tggcaacgac cacatcctcg agatcatcga
     4801 cgtcgggacc ttgccgagcg gctcgcgcta catggtgagc gagttcctcg acggcgaacc
     4861 cctttcgggc cgccttcaac gcctaggtcc gatggacccg cgcaccatcg gcgggctcat
     4921 ggtgcagctg ctgacggggc tcggcgccgc gcaccacgtc gggatccttc accgcgatct
     4981 caagccggac aacatcttcc tggttcgcga gaaggccgga aagaccgact tcgtcaaaat
     5041 catcgatttc gggatttcga agttccagtc tctgtccggg tcgtccgatt cgatgaagat
     5101 gaccgcgacc ggagtcgtgg tcggcacgcc gtactatctc tcgccggagc aggcgcgagg
     5161 agtccgcgac atcgacgcgc gcagcgatct ctatgccgtc ggcgtgatca tgtacgagtg
     5221 cgccaccggg cgcgtcccgt ttcaagccga tagcttcaat cagctgatct tcaagatcgc
     5281 gctcgagcag cctccgccgc tcgagagcat cgtgcccggc atcgacccct ggttcgtcgc
     5341 gatcgttcaa cgagcgatgg cgcgcgaccc cgcgcagcgc tttcaaagcg cgtacgaaat
     5401 gcgcgacgaa atcgcgcggt ggctcgggat gtcgcaaagc tcgggcgctc gcgccgcggc
     5461 gccgggcttc gatgcgacgg tcatggccga ggcgaacttc acgccggtgt tgcagccgcg
     5521 cgtgccgacg ccgagcagct tcggccgtac ttcggagccg ctcaccgacg agctgctcgc
     5581 gccccggcgt tcgggatcgg caaaggtcct tctcctcgca gcgcctctgg tgctcgtgat
     5641 cgcgggggcc gtgttcttca ttgtttccca tcagaatggc ggagaaccga ccgcgagctc
     5701 tgcgccatcg gcgactccgc ctgcggcgag caccgctgca gtggcgctga agcctccgcc
     5761 agagccatct gcgacggtcg acgtcgctcc gccacgcgtg gaggtcgaga cgcccgaggt
     5821 gccgctttcg gctctgcccc cgctcgaagc ggtgcggccc aactccgcga agcgattccc
     5881 cgcgcagcgt ccgacgtcgc gaccctcgcc cgccggccac gcgcgcgccg atacggcacc
     5941 cccctccgcg gtgcagcggc cggaacccga gccgcctcgt ccgacgccag cgcaacgccc
     6001 gtcgacgcgt cccaattcgg atcgcgattt cggttattga gacgcgcgac gcgcggcgcc
     6061 gagcgcgtgg ttttggcttg gccgagccgt tcaagctcga ctatgttccg ccccgtgggt
     6121 gctttgcgct tgtattctgg cgtgttcgcg ttgggagcat gcttggttgc ttcacctcga
     6181 cccgcgctag cagagtctga ccgcgatcgc gcagcggcgc gggcggcggc tgacgcgggc
     6241 gctgacgcgt tcgaccagca acggtacgcg gacgccgtgg agctcttcgg gcgcgccgaa
     6301 aagctcgtgc atgcgacgcc gcacttgctg ttcatcgcgc gctccttggt gaagctcggt
     6361 cgtttggtcg aagctcacga gacctatctc aagatccagc gcgagcggct c
//
LOCUS       DASTKS010001327         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_215383, whole genome shotgun sequence.
ACCESSION   DASTKS010001327 DASTKS010000000
VERSION     DASTKS010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215383"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..601
                     /locus_tag="VFQ35_28455"
     CDS             83..601
                     /locus_tag="VFQ35_28455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934683.1"
                     /translation="MLRILFASSLLIACATGAPAKSPEAESEPESAVQEKTPGGESSS
                     SDAPAEKASESASKPASNDDVKEVLQLVIDDEALGPYLHLDRPDRFPVRVAGSALPQG
                     IELTKATKPVVIVPESEAEKKPTIVFTEIQVGGDDASVRYRYDVEKVRGSSTLKRRDG
                     HWVLVRSRVSER"
     gene            906..1478
                     /locus_tag="VFQ35_28460"
     CDS             906..1478
                     /locus_tag="VFQ35_28460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934684.1"
                     /translation="MRKTSFGSEAAKPNRRPSRLFLGSVACALCVSAGLGGCSTAASC
                     DPEIKSGTKFKVTVIKETDKSDKCHVIKVSEFSPFMVSAAQTQPTVEHPDCSVIPAAD
                     PPPQDDVFIEGCTPGKADMLSIFCEIRYPSKCDGSMTFSFVGEKDKVVDWTAPVIENV
                     YFHIEDKAVSCLPDIANCFDEYQVKLERQN"
     gene            complement(1815..>2446)
                     /locus_tag="VFQ35_28465"
     CDS             complement(1815..>2446)
                     /locus_tag="VFQ35_28465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934685.1"
                     /translation="IHSGIDTDKFVPRPRDNALAARYGIDLERHRVFGIVARLSEEKG
                     HNYLLQAFAGVREKTPEARLLVVGDGPLGPEVRAQATSLGLDDAVIFTGTQRNIPDYL
                     ALFDVFVLSSTRESFPLAAREAMAAGRPVIAPRIGGCPEVVDEGVTGFLFESRNVDQL
                     RQRMLDMLDRERAEQMGRAARERVERLFSRRSWVEGDELVYKDWANAPT"
BASE COUNT          468 a          741 c          809 g          428 t
ORIGIN      
        1 gagagccaag aaacgcgagc gagaagcgcg cggggaactt gggacatccc ctaggtcggc
       61 gaagaccggt atcattcgcc tcatgctgag aatcctcttc gccagctcgc tactcattgc
      121 ttgcgcgacc ggagcgccag ccaagtcgcc ggaagccgag tcggagcccg aatccgccgt
      181 tcaggagaaa acccctgggg gtgaatcgag cagcagcgac gcaccggcgg agaaggcgag
      241 cgaatccgct tcgaagcctg cctcgaacga cgacgtgaaa gaagttttgc agctcgtcat
      301 cgacgacgaa gcgctcgggc cgtacctcca tctggatcgt ccagatcgct tcccggtgcg
      361 cgtcgcgggt tcggcgttac cacaaggcat cgagctcacg aaggcgacga agccggtggt
      421 gatcgtcccg gagagcgagg cggagaagaa gccgacgatc gtatttacgg agatccaggt
      481 tggcggcgac gacgcgagcg ttcgctatcg atacgacgtc gaaaaggttc gcgggtcgtc
      541 gacgttgaag cgacgcgatg gtcactgggt tctcgttcgc agccgcgtga gcgagcggtg
      601 agccgagcgg ggctcgcaaa ttttcagaac tttggccgaa aaccggtttc agtcgcgtgt
      661 cttcgcgcgc ggacgcctcg tgaaaatgcg tacagggctt tcgcgcgtct ctagaacatt
      721 tcgatatcgg gcacggttta gagccttggc acgcaaaaaa agaccctctc gcgttggacc
      781 gagctcgcgg gctgaagtaa gcgtgcgtcc ccgtttgcga acgtgacgcc taagggcgac
      841 ctttggttac ttgttcctta actcgcggtg ggcgagccca gtgactccga gagagagccg
      901 ttagcatgcg aaagaccagc tttggaagtg aagccgcgaa gccgaaccgc cgtccctcgc
      961 gcctcttcct ggggagcgtg gcgtgcgcgt tgtgcgtgag cgcgggcctg gggggctgtt
     1021 cgaccgccgc ctcgtgcgat cctgagatca agagcgggac caagttcaag gtcaccgtga
     1081 tcaaggagac cgataagtcg gacaagtgcc acgtcatcaa ggtctcggag ttcagtccgt
     1141 tcatggtgtc ggcggcacag acacagccga cggtcgagca ccccgattgt tcggtgattc
     1201 ccgcggccga cccgcctcct caagacgatg ttttcatcga gggttgcacg ccgggcaaag
     1261 ccgacatgct cagcatcttc tgtgagattc gttacccctc gaagtgcgat gggagcatga
     1321 cgttctcgtt cgtcggcgaa aaggacaagg tcgtcgactg gaccgcgccc gtcatcgaga
     1381 acgtctactt tcacatcgaa gacaaggccg tgagctgcct gcccgacatc gcgaactgct
     1441 tcgacgaata ccaagtcaag ctcgagcgtc agaattagta ggctgggtcc ttcggaccag
     1501 cctcgctgtt tgtcgggagc gccgctcccg cgtacgcgac gaggcggcag gtagcggcgt
     1561 ttggggccgg ggccccaaac gcaacacaaa ccaagcatcg gaatcagcct ttcgcagcca
     1621 atgaggcgac aacccttagt cgccgagcct tgagcgagac atgacgcctt ggttgcggga
     1681 agccactcgc gcagccaacg cctgagatcg cttggccccg accccaaaac ggctgggtcc
     1741 ttcggaccag cctcgctgtt tgtcgggagc gccgctcccg cgtcgcgacg aggcgcctcc
     1801 gttcgagcgg cttttcaggt cggagcgttc gcccagtcct tgtagacgag ctcgtcgcct
     1861 tcgacccaag agcggcgcga gaataagcgt tccacgcgtt cgcgcgcggc gcggcccatt
     1921 tgctctgcgc gctctcggtc gagcatgtcg agcatgcgct ggcggagctg atcgacgttg
     1981 cgcgactcga acaagaagcc ggtgacgccc tcgtcgacga cctcggggca cccgccgatt
     2041 cgcggcgcga tgacggggcg acccgcggcc atggcttcac gcgcggcgag cggaaatgac
     2101 tcgcgcgtcg acgacaggac gaagacgtcg aagagcgcca agtagtcggg gatgttgcgc
     2161 tgggtgccgg tgaagatgac ggcgtcgtcg agcccgaggc tcgtcgcttg ggcgcgcacc
     2221 tcggggccga gcgggccgtc accgaccacc aacaagcgcg cttcgggggt cttttcgcgg
     2281 acgccggcga aggcctgcaa gaggtagttg tggcccttct cctccgacaa gcgcgcgacg
     2341 atgccgaaca cgcggtgacg ctcgagatcg atgccgtagc gcgcggcgag ggcgttgtcg
     2401 cgcggccgcg gcacgaactt gtcggtgtcg attccgctgt ggatga
//
LOCUS       DASTKS010001328         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_436943, whole genome shotgun sequence.
ACCESSION   DASTKS010001328 DASTKS010000000
VERSION     DASTKS010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436943"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFQ35_28470"
     CDS             complement(<1..510)
                     /locus_tag="VFQ35_28470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934686.1"
                     /translation="MNNSHVVRIGSNTPNDSAIAAATPGQVSSLLTAGNFGSFSPETA
                     MVYLKSRLDYLDQQIQDVFNREQLGQHVRSEVHAIQMILSQAEVSGADPKATGTLSAD
                     AVAQINDHLEEISHYDLNLANELKQKLYGDGQVLRNAPDGTSPNLASPRDYRPGGTPP
                     DAEQDQPPPP"
     gene            complement(572..766)
                     /locus_tag="VFQ35_28475"
     CDS             complement(572..766)
                     /locus_tag="VFQ35_28475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934687.1"
                     /translation="MDNPLEQFQQARARQDLEWERVFAALQELDPALTFLVQRSALDE
                     LEKCFESRPVHIPSVTALRA"
     gene            878..2860
                     /locus_tag="VFQ35_28480"
     CDS             878..2860
                     /locus_tag="VFQ35_28480"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9934688.1"
                     /translation="MKLHEAVQEAVPGARVSSEAPDRRAYSRDLWPRGLIEMQSGLPE
                     REGPGCIVWPETVEHVAALVALARREGLSLVPFGAGSGVCGGAIPTAGQVVVDLKRFA
                     DHRVLAGPELDVGAGAMGITLEERLLEAGYTTGHYPSSILCSTVGGWVAARGAGQCSG
                     RYGKIEDMITSAEVVLGTGDVVRAEAHRGGPELLPLLVGSEGTLGIITRVGLRLHPAP
                     RARAFAAHSFPNVELGIETLRALFQEGLRPAVARLYDPLDSALLAPDDKPKVASPPRA
                     LKPTAALLRGIARHPALTARLMDAAERSFMSKVTLVLVHEGDEREVADEARRAEAICR
                     ARQSTPLGEGPARAWLHHRYHVSYRQMPQFRAGLFIDTMEVSAPWKHLTDVYYAVKRA
                     LGEHVLVMAHLSHSYPDGGSIYFTFAGTQHGDESPLEVYDHAWRVALAAALDSGASLS
                     HHHGVGRSKAPRLGEELGSALWVQRALKSAWDPDGILNPGVLLPSSSNADEGVPDPPT
                     QPELDAQSGLVTLPGTMLLGDAERWLRERAHSLHLPSLPELSVDDFVARGLPGAPDRF
                     DDPVGTRLAGFSARLSSGRRFAIKAAPRRAVGPDLSALFVGARGAFGRIESARLSALP
                     QSASPAPRRAFDKPANPNLNADEAAAFERLRQALST"
     gene            complement(2850..4697)
                     /locus_tag="VFQ35_28485"
     CDS             complement(2850..4697)
                     /locus_tag="VFQ35_28485"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HET9934689.1"
                     /translation="MVRLRHALPLEAWAQTLAGCAEIPEQQLDAYFRDTLLRAGARRL
                     LANHGLEPPPYARLFDLVGDAVRVDAWIEEDAERTRAREAATATLGSFIADRDLPHAL
                     GALFEALSSFEVRSEGGEVRLELTRRRKKTGMFYTPSAIAHEVANRALEAFGAVPSSV
                     CDPACGSGAFLIEAARALALKLGDRRAAAARLTGVDLERRALLVAELALWAFVGDPSW
                     DGAGLRLLEGDALSASFDWKQVAGTGFDWVVGNPPWVAFAGRAAQPLAPSKKAHYLAE
                     FVSFRGYPTLHALFVERALSLAPSGVISLLVPSPVADLDGYRAMRRRLLATHEPCEPL
                     LEFGQDAFETVTQPCFALIAKARPDPAPADERRLRLAERELLGKQAKTVAPPEVLLRL
                     ARGEVFPRELFGELGFQSNRDVSTRLFLRAESARAPFTYPLLEGREVSEFRVGEPRLF
                     LHPDESLLKKTRCKLRLVEDYRRVGFVVRQTAKYPIAAVHTGLPFRNTLLAGFAVHEL
                     APEFVVGLLNSSLYRALHLASQRDARQKVFPQVKVAHLRALPRPPSGRQALRERVAEL
                     TREAARLHIAELRRELDEAVFALFDLASSEREQVLRFVAERAPELAIKY"
     gene            complement(4699..>5227)
                     /locus_tag="VFQ35_28490"
     CDS             complement(4699..>5227)
                     /locus_tag="VFQ35_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET9934690.1"
                     /translation="ADRLVTSLVTTHVPLGRVPGELTAEGVSRATTELVRLLRASGKQ
                     KPRIAVASLNPHAGESELLGDEERRAIVPGIRLARRRTGKHAEILGPIGAETAYRKAY
                     AGDYAGVVAMYHDQATIPLKLVAFGDAVNVTMGLSIVRTSVDHGTAYDIAWTGRADAE
                     GMLSAMRLGARLNES"
BASE COUNT          824 a         1781 c         1774 g          848 t
ORIGIN      
        1 gggcggcggt ggctggtctt gctccgcgtc aggaggtgta ccgcccgggc gatagtcgcg
       61 gggcgaggcc aaattgggtg aggttccgtc cggtgcgttg cgcaaaacct gcccgtctcc
      121 gtagagcttc tgcttcagct cgttcgcgag gttgaggtcg tagtgtgaaa tctcctcgag
      181 gtgatcgttg atttgggcga cggcatcggc gctgagggtc cccgtcgcct tcgggtctgc
      241 tccggaaact tcagcttggg acaagatcat ctggatggcg tgaacctccg aacgaacgtg
      301 ttgccccagc tgctcgcggt tgaacacgtc ttgtatctgc tggtcgaggt agtcgaggcg
      361 gctcttgagg tagaccatcg cggtctcggg tgaaaaagag ccgaagttcc ccgcggtgag
      421 cagcgagctc acttgtccgg gtgtggcggc cgcgattgcc gaatcgttgg gagtgttcga
      481 accgatccgc acgacgtgcg aattgttcat agcgagtagg tttgggactt ggggagcaac
      541 agttgtcatt tcgagcgttc ctttcgcaat ctcaggcgcg cagcgccgtc acggacggga
      601 tgtggacggg cctcgattcg aagcacttct cgagctcgtc gagcgcgctt cgctggacca
      661 gaaacgtgag cgccgggtcg agctcttgga gcgccgcgaa cacgcgctcc cactcgaggt
      721 cctgacgggc gcgcgcttgc tgaaactgct cgagcggatt gtccatgtga ggctcctttt
      781 ggggcgggtt tgccgccgtg ccccctccgt cggtcacctc ccggggcggt tgctcgattt
      841 cgttcgggcc gcgtagtttt tcggtaagct cgggcgggtg aagctccacg aggcggtaca
      901 agaggcggtg cccggcgcgc gcgtgagctc cgaagcgccc gaccgccgcg cctattcgcg
      961 cgatctctgg ccgcgcgggc tcatcgagat gcagtcgggg ctacccgagc gcgaaggccc
     1021 tgggtgcatc gtgtggccgg agacggtgga gcacgtagcc gcgctggtcg cgctcgcgcg
     1081 acgtgagggc ctgtcgctcg tgccgttcgg ggctggcagc ggcgtgtgcg gcggtgcaat
     1141 accgaccgcg gggcaagtcg tcgtcgattt gaagcgcttt gccgatcacc gggtgctcgc
     1201 ggggccggag ctcgacgtgg gcgcgggcgc catgggcatc acgctcgaag agcgcttgct
     1261 cgaggctggc tacacgacgg gacactaccc ttcgagcatc ctttgctcca cggtcggagg
     1321 ctgggtcgcc gcccgcggcg ccgggcaatg ctccgggcgc tacggcaaga tcgaagacat
     1381 gatcacgagc gcggaggtcg tgctcggcac cggcgacgtc gtgcgcgccg aagcgcatcg
     1441 aggcggaccc gagctgcttc cgctcttggt cggcagcgag ggcacgctcg gcatcatcac
     1501 gcgcgttggt ttgcgcctgc atccggcgcc ccgcgcccgc gcctttgcgg ctcattcatt
     1561 cccgaacgtc gagctcggca tcgaaacgct gcgcgcgttg ttccaagagg ggctgcggcc
     1621 cgcggtcgcg cgcctgtacg atccgctcga cagcgcgctg ctcgctcccg acgacaagcc
     1681 gaaggttgcg tcgccgccgc gcgcgctcaa gccgacggcc gcgctactgc gcggcatcgc
     1741 gcggcaccca gcgctcacgg cgcggctgat ggacgcggcc gagcggagct tcatgtccaa
     1801 agtgacgctg gtcctcgtac acgagggcga cgagcgcgaa gtcgccgacg aagcgcgacg
     1861 cgccgaagcg atctgccgcg cacgccagag cacgccgctc ggcgaagggc cagcgcgcgc
     1921 gtggctgcac catcgctacc atgtgagcta ccggcaaatg ccgcaatttc gcgccgggct
     1981 cttcatcgac acgatggagg tctccgcgcc gtggaagcac ctgaccgacg tgtattacgc
     2041 cgtgaagcgc gcgctcggtg agcacgtgct cgtgatggcg cacctgagtc actcctaccc
     2101 cgacggcggc agcatctatt tcacgttcgc cggcactcaa cacggcgacg agagcccgct
     2161 cgaagtgtac gaccatgctt ggcgcgtggc gctcgcggcc gcgctcgaca gcggcgcgtc
     2221 gctcagccac caccacggcg tcgggcgcag caaggcgccg cggctcggcg aagagctcgg
     2281 cagcgcgctt tgggtgcaac gcgccttgaa gtcggcgtgg gatcccgacg gcattttgaa
     2341 tcccggcgtg ctcctgccgt cgtcgtccaa cgcggacgag ggcgttccgg atccgccgac
     2401 ccagcccgaa ctcgacgcgc agagcggact cgtgacgttg cccggaacga tgctcctcgg
     2461 cgacgccgag cgctggctgc gcgaacgcgc gcacagccta catcttccgt cgctaccgga
     2521 gctcagcgtc gatgatttcg tggcgcgcgg ccttcccggc gctccggatc gcttcgacga
     2581 ccccgtcggg acgcgcctgg ccggcttttc ggcgcgactc tcgagcggac gtcgctttgc
     2641 catcaaggcg gcgccccgcc gcgcggtggg accggacctc tcggcgcttt tcgtgggagc
     2701 acgcggcgca ttcggccgca tcgagtccgc gcggttgagc gcgctgccgc agtcggcctc
     2761 gccagcgccg cgccgcgcgt tcgacaagcc cgcgaacccg aatctcaacg ccgacgaagc
     2821 cgccgcgttc gagcgattgc gccaggctct cagtacttga tggcgagctc gggagcgcgc
     2881 tccgcgacga agcgcagcac ctgctcgcgt tcggagctcg ccaagtcgaa cagagcaaag
     2941 acggcttcat cgagctcacg gcgaagctcg gcaatgtgca gtcgtgccgc ttcgcgcgtc
     3001 agttcggcga cgcgttcgcg cagcgcctgc cttcccgacg gcggccgagg cagcgctcgc
     3061 aaatgggcga ccttcacctg agggaagacc ttttggcgcg cgtcgcgctg cgaagcgaga
     3121 tgcagcgctc gatacaagct gctgttcaag agcccgacca cgaactccgg cgcgagctcg
     3181 tgaacggcga acccggcgag gagcgtattg cgaaacggta agcccgtgtg aacggccgcg
     3241 atcggatact tcgcggtttg ccgcacgacg aagccgaccc ttcggtagtc ctcgacgagc
     3301 cgcagcttgc agcgcgtctt cttcagaagg ctctcgtcgg ggtgcaggaa caaacgcggc
     3361 tctccgacgc gaaactcgct cacttcgcgg ccttcgagca gcgggtacgt aaacggcgct
     3421 cgcgcgctct cggccctcag aaagagccgc gtgctcacgt cccgattcga ttgaaacccg
     3481 agctctccga acagctcccg cgggaagacc tcgccgcgcg ccagacgcaa taacacttcg
     3541 ggcggagcta ccgtcttcgc ttgcttgccg aggagctcgc gctccgccaa acgcaagcgc
     3601 cgctcgtccg caggcgcggg atcggggcgc gccttcgcga tcagcgcgaa gcacggctgc
     3661 gtcaccgttt cgaaggcgtc ttgaccgaac tcgagcaacg gctcgcaggg ctcgtgcgtg
     3721 gcgagtaaac gccggcgcat cgctcggtag ccgtccaagt cggcgaccgg gctcggcacc
     3781 aagagcgaga tcacaccgct cggcgcaagc gagagggcgc gctctacgaa gagggcgtgc
     3841 agcgtcgggt aaccgcgaaa actcacgaat tcggcgaggt aatgagcctt cttcgagggc
     3901 gcgagcggct gcgcggctcg gccggcgaaa gcgacccaag gcggattgcc gacgacccaa
     3961 tcgaagccgg ttccggcgac ctgcttccaa tcgaaactcg ccgagagcgc gtcgccttcc
     4021 agcaagcgga ggccggcgcc gtcccagctg gggtcgccga cgaacgccca cagcgcgagc
     4081 tcggcgacga gcagcgcgcg ccgctccaaa tcgacgcccg tgagccgcgc cgccgcggcg
     4141 cgccggtcgc cgagcttcaa cgcgagcgcg cgcgcggcct cgatcaggaa tgctccggag
     4201 ccgcacgccg gatcgcacac ggagctcggc acggcgccga acgcttcgag cgcacggttc
     4261 gcgacctcgt gggcgatcgc gctcggcgtg tagaacatgc ccgtcttctt gcggcgccgc
     4321 gtgagttcga gccgaacctc gccgccttcg gagcgcacct cgaagctcga cagcgcttcg
     4381 aacagggcgc cgagcgcgtg cggcaagtcg cggtcggcga tgaagctgcc gagggtcgcc
     4441 gtcgcggctt cacgcgcgcg cgtgcgctcg gcgtcttctt cgatccaagc atctacgcgc
     4501 accgcgtccc ccaccaaatc aaacagccgc gcgtagggag gaggctccaa gccatggttc
     4561 gcgagcaacc ggcgtgcccc cgcgcggagc aacgtatctc gaaagtacgc gtccagctgc
     4621 tgttccggga tttccgcgca tcccgcgagc gtctgcgccc aagcttcgag cggcagcgcg
     4681 tgacgcagcc gaaccatctc agctctcgtt cagccgagca ccgagccgca tcgccgatag
     4741 catcccttcg gcgtcggcgc ggcccgtcca cgcgatgtcg tacgcggttc catgatcgac
     4801 gctcgtgcgc acgatgctga gccccatcgt gacgttcacg gcgtcgccga aggccacgag
     4861 cttcaacgga atcgtcgcct ggtcgtggta catcgcgacg acgccggcgt agtcgccagc
     4921 gtaggctttg cggtacgccg tctccgcacc gatcggcccc aagatctcgg cgtgcttgcc
     4981 ggtgcggcgc cgcgcgagac gaatgccggg cacgatcgcg cgtcgctctt cgtcgcccag
     5041 gagctcgctc tcaccagcgt gcgggttcaa cgacgcgacg gcgatgcgcg gcttttgctt
     5101 gccgctcgcg cgcagcaaac gaacgagctc ggtcgtcgcg cggctcacgc cctcggctgt
     5161 cagctcgccc ggcactcgac cgagcggaac gtgcgtcgtc acgaggctcg tcaccaaccg
     5221 atccgcc
//
LOCUS       DASTKS010001329         6890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_215628, whole genome shotgun sequence.
ACCESSION   DASTKS010001329 DASTKS010000000
VERSION     DASTKS010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215628"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFQ35_28495"
     CDS             complement(<1..548)
                     /locus_tag="VFQ35_28495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9934691.1"
                     /translation="MGDPSETPAAPPDPVLVAAPRQVPWLAVLTVAAALATTLGSVSL
                     AGIWDSPEVAHAEFGRRIAHDLLGASELGGPGEPALTREALGRGELPFLSIALGFRLF
                     GLSTQAGRVALALWGLAGIAALHWLVRRLGSLRAAWLAALALATSPLYFVQARTMVGD
                     VVGMSANAVAMAGLALAVFDGGS"
     gene            685..1620
                     /gene="era"
                     /locus_tag="VFQ35_28500"
     CDS             685..1620
                     /gene="era"
                     /locus_tag="VFQ35_28500"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9934692.1"
                     /translation="MRFGTVALFGRSNVGKSTFLNAVLEQDLAIVSPLPQTTRDALLG
                     VVSRKDAQIAFVDTPGVHRPKSELGRRMNHAAFETARTTDVVIFMTDTSSLARRVGKT
                     REVGPDPEDVRLIGELPANVPALLVINKVDLLRDKARLLPALQGFEKLRNWSALIPIS
                     ALTDDGVERVLAEIVPLLPEGDAGYSTDTLTDRPTSYFVREYVREQVLAATQGEVPHA
                     VAVSIDRYEEGKLPRIFATLHVEKEGQRRILVGDGGSKIRDIGSAARARIESLLGTQI
                     YLELFVRVSPRWKDAPRQLAELGYVKGKGDVEERK"
     gene            1617..2933
                     /gene="der"
                     /locus_tag="VFQ35_28505"
     CDS             1617..2933
                     /gene="der"
                     /locus_tag="VFQ35_28505"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9934693.1"
                     /translation="MIPIVAIVGRPNVGKSTLFNRLCGKSLAIVHDEAGVTRDRHYAP
                     AHLAGRSVTLIDTGGFDPTSDDPMQAGIARQVEVAIEEADVVVCVLDATTPPTGPDGD
                     AVRLLRQSGKAVIYVANKVETKEHEFALGPLYELGVPSLIAVSALHGRGTAELANAIR
                     EHLPTDESEPEAEPSEVPRIALVGRPNAGKSSLFNRLVGEERSLVDSRPGTTRDPVDE
                     LVEFEGERFTIVDTAGIRRRSRVERGIELVSVLRSIRAVERAEICVLMCDATEGVTEQ
                     DARLLALCLERRRGVVVGLNKMDALDAKGRKRAIEQAEETLHFARFARIVPISAKRNE
                     NLGTLMRVAAKAAREMRKRISTAELNRFFENVLDKRPPPTHGGRAPRIYYITQAETSP
                     PVFIAMSNHPEHLNESYQRFVVNQIREVFGFESVPVTVQYRQKRAH"
     gene            complement(2955..4103)
                     /locus_tag="VFQ35_28510"
     CDS             complement(2955..4103)
                     /locus_tag="VFQ35_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET9934694.1"
                     /translation="MSRPALAKLSSERRVLVVVGAGGVGKTTVAAALGLAAAVRGLNV
                     LCLTIDPAKRLLDRLGVDASGAAEQLIDARKFADEGLELKGRLSVSMLDTKRTFDDLV
                     RRHASSKEAAERILANEFYEYVSTQLAGTQAYMAMEKVLSVLEERRYDLVVLDTPPTS
                     DALDFLDAPARLIETLDSAALRWLAEAFERSGRLGLNWMARGVSLVLRGIARLTGRRF
                     LERLSEFVSELNELFGGFKERAERVARAFRGPEFAYLLVATPSSESLDEARFFAERLA
                     RTGMRADGLIINRVRVDPGSAPSPEELAAACAAHGLSAAGDFASRVLSAQADEAEAVT
                     IDRRNLAVARSTASELAPERIPFAIEVPAFPASVHDVAALVSVCRQLM"
     gene            4193..4546
                     /locus_tag="VFQ35_28515"
     CDS             4193..4546
                     /locus_tag="VFQ35_28515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934695.1"
                     /translation="MQAPTPERRVQRSSNPSEALVCWLEAVRRRHALRSLALADSLGT
                     VVAGAGPARECDELAAWAPLFLGDEGSRAGAPFELSLVPVPGFDAYVCAENVAANDVL
                     SDAAAGCSRILGARV"
     gene            4607..5329
                     /locus_tag="VFQ35_28520"
     CDS             4607..5329
                     /locus_tag="VFQ35_28520"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HET9934696.1"
                     /translation="MRGLIDLHCHFVAGIDDGARTEEEGIELLRALYRLGFDHVVATP
                     HMRPGMFDNDKLALRTAYDAVVPKLVGPGFPETDLSSEHYFDDVVFSRIMNGEGLPYP
                     GGKAVLLEFYSVDFPPAVDMCFSRLRRSGILPVIAHPERYQCLWQRTDRLERLIDGGA
                     ATLLDTAALVGKYGAMPQRTAERLLEEGLYHAACSDAHRPADVRAVESGMKRIEELYG
                     EEEVELLFVEGPRALLAGKLPD"
     gene            5409..5882
                     /locus_tag="VFQ35_28525"
     CDS             5409..5882
                     /locus_tag="VFQ35_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934697.1"
                     /translation="MLRRLSAALLCAFASTIAGNAAARVEASSGYSKPQTFSCALRLL
                     RVDRGYEVVEKDQDAAYVLFRYPISGQKEQASGSLEIVETAAGVRVFVQLPKLPEYHE
                     ALLRDALLRKLREEYGPPPNKPPAEKPKAPPDKEKSKPKGDAPEGSDEPPRDGSS"
     gene            6034..>6890
                     /locus_tag="VFQ35_28530"
     CDS             6034..>6890
                     /locus_tag="VFQ35_28530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934698.1"
                     /translation="MEGADTSLPSTIGRYEVVRLLGRGAMGRVLLARDPVLNRDVAVK
                     LLRDDLRLPPEQLDGLVERMRQEARASARIVHPNIVALFDMGEEPDLGLFLVFEYADG
                     PTLKERLKRSPVGPQAAAKLAREIGDALSTAHAAGVLHRDIKPDNVILTATGSKIADF
                     GIARVPDSTLTRDGGLLGTPAYSAPEAILHGTFSPASDQFSMAATLYEALSQTRAFPG
                     ADAVTVANRITTEDASPIAAACGLNPHVDEVLMRALARNPADRYESAREFGYALAEGL
                     EVAPRTAMAT"
BASE COUNT         1220 a         2364 c         2269 g         1037 t
ORIGIN      
        1 gacccgccgt cgaacacggc aagcgcgagg ccagccatcg cgacggcgtt cgcggacatg
       61 ccgacgacgt cgccgaccat cgtgcgcgct tgcacgaagt agagcggcga cgtcgcgaga
      121 gcgagcgcgg cgagccaggc cgcgcggagc gagccgagtc ggcggactag ccagtgaagc
      181 gcggcgatgc cggcgagccc ccagagcgcg agagcgactc gcccggcctg cgtggacaac
      241 ccgaacagcc gaaagccgag cgcgatggac aagaacggca gctccccgcg tccgagcgcc
      301 tcgcgcgtga gcgccggttc ccccgggccc cccagctcgc ttgctcccag caagtcgtgg
      361 gcgatgcgcc ggccgaactc ggcatgagcg acctccggcg aatcccaaat cccggcgagc
      421 gagacgctgc ccaaggtagt cgcgagcgcc gccgcgacgg tcaaaacggc gagccatggc
      481 acttgacgcg gcgctgctac gagcaccggg tcgggcgggg ctgctggggt ttctgagggg
      541 tcgcccatgc gcggagaacc gaaccttcgc gaaagcggcg gccccgggca agcccaatta
      601 ccgggaattt cgcagcacta acgcaacatc gagagctcag gcggttgcag ccggcgacgc
      661 tcgggggtag cccgctcggc ccctatgcgc tttggcacgg tcgccctatt cggtcgcagc
      721 aacgtgggaa agtccacgtt cctgaacgcg gtcctcgagc aggatctcgc catcgtctcg
      781 ccgttgcccc agacgacgcg ggacgcgttg ctcggcgtcg tgagtcgcaa ggacgcgcaa
      841 atcgcgttcg tggacacgcc cggcgtgcat cggcccaagt ccgagctcgg ccgtcggatg
      901 aatcacgccg cattcgagac ggcgcgcacc accgacgtcg tcatcttcat gaccgacacg
      961 agctcgctcg cgcggcgcgt cggcaaaacg cgcgaagtgg gtcccgatcc cgaggacgtt
     1021 cggctcatcg gagagctccc cgccaacgtc cccgcgctac tggtcatcaa caaggtcgac
     1081 ctgctgcggg acaaggcgcg cctcttgccg gcgctgcagg gcttcgaaaa gctgcgcaac
     1141 tggagcgcgc tcatcccaat cagcgcgctc accgacgacg gcgtcgagcg agtgctcgcg
     1201 gaaatcgttc ctctcttgcc cgaaggcgac gccggctact ccaccgatac actcaccgac
     1261 cgcccgacga gctacttcgt gcgagagtac gtccgcgagc aggtgctcgc cgcgacgcag
     1321 ggcgaagtgc cgcacgccgt tgcggtgagc atcgaccgct acgaggaggg caagctgccc
     1381 cgcatcttcg cgaccctgca cgtcgaaaaa gaaggacagc ggcgcatctt ggtcggcgat
     1441 ggcggcagca agatccgcga tatcgggagc gccgcgcgcg ctcgcatcga gtcgctgctc
     1501 ggcactcaga tttacctcga gctcttcgtt cgggtgagcc ctcgctggaa ggacgctccc
     1561 cgacagctcg cggagctcgg ctatgtgaag ggcaaagggg acgtggagga acgcaaatga
     1621 tccccatcgt agccatcgtc ggtcggccca acgtcggcaa gagcaccctc ttcaatcgcc
     1681 tgtgcggcaa gagcctcgca atcgtacacg atgaggcggg tgtcacgcgc gatcgccact
     1741 acgcgcccgc gcacctcgca gggcgctccg taacactcat cgacacgggc ggcttcgacc
     1801 cgaccagcga cgatccgatg caggcgggca ttgctcgcca agtcgaagtc gcgatcgaag
     1861 aagcggacgt ggtcgtgtgc gtgctcgatg ccacgactcc gccgaccggc ccggacggcg
     1921 acgccgtacg gctcctgcgg cagagcggca aggccgtcat ctacgtcgcg aacaaggtcg
     1981 agacgaagga gcacgagttc gctctcggcc cgctctacga gctcggcgtc ccgagcctca
     2041 tcgccgtctc cgcgctacac ggccgcggca ccgcggagct cgcgaacgcc attcgagagc
     2101 acctccccac cgacgagagc gagcccgagg ccgagccgag tgaggttccg cgcatcgcgc
     2161 tcgtagggcg accgaacgcg ggaaagtcct cgctcttcaa tcggctggtg ggagaagagc
     2221 gttcgctcgt cgattcgcgt ccgggcacga cgcgcgaccc ggtcgacgag ctcgtcgagt
     2281 tcgaaggcga acgcttcacg atcgtggaca ccgctggcat tcggcgacgc tcgcgcgtcg
     2341 aacgtggaat cgagctcgtc agcgtgctgc gttcgatccg cgccgtggag cgcgccgaaa
     2401 tatgtgtctt gatgtgcgac gccaccgagg gcgtcacgga gcaggacgcg cgcctgctcg
     2461 cgctctgcct cgagcggcgg cgcggtgtcg tcgtcggctt gaacaaaatg gatgcgctcg
     2521 acgcaaaagg ccgaaagcgc gccatcgagc aggccgaaga gacccttcac ttcgctcgct
     2581 ttgctcgcat cgttcccatc agcgcgaaac gcaacgaaaa cttgggcacg ttgatgcgcg
     2641 tcgcggccaa ggcggcgcgc gagatgcgca agcgaatctc gaccgcggag ctcaaccgct
     2701 tcttcgagaa cgtgctcgac aagcgcccgc cgcccacgca cggcggacgc gcgccgcgca
     2761 tctattacat cacgcaggcg gagacctcgc cccccgtgtt catcgcgatg agcaatcacc
     2821 ccgagcactt gaatgagagc taccagcgct tcgtggtgaa ccagatccgc gaggtgttcg
     2881 gcttcgagtc ggtgccagtg accgtgcagt accggcaaaa gcgagcgcac tgacgcgcgg
     2941 agcgagcctg ctcctcacat cagctgccgg cagacgctga cgagcgccgc gacgtcgtgc
     3001 acgctcgcgg ggaacgcggg cacctcgatc gcgaacggga tgcgctcggg cgcgagctca
     3061 ctcgcagtag agcgcgcaac cgccaggttt cgacgatcga tggtgacggc ctctgcctcg
     3121 tcggcctggg ccgagagcac cctggaagcg aaatcccccg ccgccgaaag gccgtgcgcg
     3181 gcgcacgccg cggcgagctc ttctgggctc ggagcggagc cggggtcgac gcgaactcgg
     3241 ttgatgatga gcccgtccgc gcgcatcccc gtgcgcgcca gacgctctgc gaaaaagcgc
     3301 gcttcgtcga gcgattcact cgacggcgtc gcgaccaaca agtacgcgaa ctccggcccg
     3361 cgaaacgcgc gcgcgactcg ctcggctcgc tctttgaagc cgccgaagag ctcgttcaac
     3421 tcactcacga actccgagag ccgctcgagg aatcgcctcc cggtgagccg cgcgatgccg
     3481 cgcagcacca aggacacgcc gcgcgccatc caattgagcc cgagccgacc cgagcgctcg
     3541 aaggcttccg cgagccaccg cagcgccgcg ctgtccagag tttcgatgag ccgcgccggc
     3601 gcgtccaaga agtcgagggc atcgctcgtc ggaggcgtgt cgagcacgac caagtcgtag
     3661 cggcgctctt cgagcacgct gagcaccttc tccatcgcca tgtaggcctg cgtgccggca
     3721 agctgggtcg atacgtactc gtagaattcg ttggcgagga tccgctcggc tgcctccttg
     3781 ctcgaggcgt ggcgtcgcac gaggtcgtcg aacgtgcgct tcgtgtcgag catggagacg
     3841 ctgaggcgcc ccttcaactc gagaccttcg tcggcgaact tgcgcgcgtc gatgagctgc
     3901 tccgcggcgc cgctcgcgtc gacgcccaat cgatcgagga gccgtttggc gggatcgatg
     3961 gtgaggcaaa gcacattcag cccgcgcacg gcagcggcga ggccgagcgc cgccgcgacc
     4021 gtcgtcttcc ccaccccgcc cgcgcccacc acgacgagca cgcggcgttc cgacgacagc
     4081 ttggcgagcg cagggcgaga cattacgagg tatttaacac cgggcagagc cgggtcgctc
     4141 gatgagcgga cgaaagtttg gccgggctcc agggccgccc gataatgggc tcatgcaggc
     4201 tcccaccccg gaacggcgtg ttcagcgttc gtcgaacccg agcgaagcgc tcgtgtgctg
     4261 gctcgaggcc gtacgtcggc ggcacgcgct tcgtagcctc gccttggccg actcgctcgg
     4321 caccgtggtc gccggagcgg ggccggcgcg cgaatgcgac gagctcgccg cgtgggcacc
     4381 gctgtttctc ggcgacgaag gctctcgcgc gggagcgccc ttcgagcttt cactggtgcc
     4441 ggtgcctggc ttcgacgcct acgtttgcgc cgaaaacgtc gctgccaacg acgtgctgag
     4501 cgacgccgcc gcgggctgtt cccgaatcct tggcgcccgc gtctagagcg cacgagcctc
     4561 cgcgcgaccc agggtgggcg aaaatcgggt aagctgcgcg tcacccatgc gcggcttgat
     4621 cgatttgcac tgccacttcg tggctggaat cgacgacggc gctcgcaccg aggaagaggg
     4681 aatcgagctc ttgcgagcgc tgtaccggct cgggttcgac cacgtcgtcg cgacgcctca
     4741 catgcggccc ggcatgttcg acaacgacaa gctcgcgttg cgcaccgcgt acgacgccgt
     4801 cgtgcccaag ctcgtgggcc cggggttccc ggagaccgac ctctcgagcg aacactactt
     4861 cgacgacgtc gtgttctcgc gcatcatgaa cggtgaaggg cttccctacc cgggcgggaa
     4921 agccgtgctg ctcgagtttt actcggtcga cttcccgccc gcggtggaca tgtgcttttc
     4981 gcgcctgcgg cgctccggca tcttgcccgt gatcgcccac ccggagcgct accagtgcct
     5041 gtggcagagg acggatcgcc tcgagcggct catcgacgga ggtgccgcga cgctgctcga
     5101 cacggctgct ctcgtcggga aatacggcgc gatgcctcag cgaacggccg agcgcttgct
     5161 cgaggagggg ctctaccacg cggcgtgcag tgacgcgcac cgccccgccg acgtccgcgc
     5221 ggtcgagagc ggaatgaagc gcatcgagga actctacgga gaagaagaag tcgagctcct
     5281 cttcgtcgaa gggccgcgcg cgcttctcgc cgggaagctt ccggactgag ttgttcgagc
     5341 acactgccgg atcttcgttc cgcgcagaga acttggcccg cgcaggcgaa ggcggatagg
     5401 ccatcgccat gctgcgccga ctctccgctg ccctgctctg cgcgttcgcg agcaccatcg
     5461 cggggaacgc cgccgcacgg gtcgaagcga gctctggcta cagtaagccg cagacgttca
     5521 gttgcgctct ccgcctgctt cgcgtcgatc gcggctacga agtcgtcgag aaagatcagg
     5581 acgcggccta tgtcttgttt cgctacccca tcagcgggca gaaggaacaa gcgagcggat
     5641 cgctcgagat cgtggagacg gccgctggcg tacgggtctt cgtccagctc ccgaaactac
     5701 ccgagtacca tgaggcgttg ttgcgggacg ccctgctgag aaaactccgc gaagagtacg
     5761 gaccaccgcc aaacaagccc cccgcggaga aacccaaagc gccgcccgac aaagagaaat
     5821 cgaagccgaa gggcgacgct ccggagggtt cggacgagcc ccctcgcgac ggttcctcgt
     5881 agagcgcagc cccgcgctgc tgtcgccgcg caaaattcct cgagcgcgca cgctcctttc
     5941 cttggcgcgt cccaaacggc ccgtgaaacc gccaccggcc gcgggtttgg aattggcacg
     6001 ccgcggcgag catgaataga tgccaaggct cgcatggaag gtgcggacac ctcgttgcca
     6061 tccacgatcg gccgctacga agtcgtgcgg ctgctcggcc gtggcgccat gggtcgagtg
     6121 ctcctggcgc gcgatccggt actgaaccgc gatgtcgcgg tgaagctgct gcgcgacgac
     6181 ctgcgactac cgccggagca gctggacggt ctcgtggagc gcatgcgcca agaagcgcga
     6241 gcgtccgcgc gcatcgtgca cccgaacatc gtcgcgctct tcgacatggg cgaagagccg
     6301 gacctcggct tgttcctggt attcgagtac gccgacggcc ccacgttgaa ggagcgactc
     6361 aagcggagtc cggtggggcc acaggcggcg gccaagctcg cgcgcgaaat cggcgacgcg
     6421 ctgtcgaccg cgcatgccgc cggcgtcctg catcgagaca tcaaacccga caacgtgatc
     6481 ctcaccgcca ccggcagcaa aattgccgat ttcgggatcg cgcgcgttcc tgattcgacg
     6541 ctgactcgcg atgggggcct gctcgggacg cccgcgtaca gcgctcccga agcgattttg
     6601 cacggcacgt tctcgccagc gagcgaccag ttctcgatgg cggcgacgct ctacgaagcg
     6661 ctcagtcaaa cccgcgcttt cccgggtgcg gatgcggtca cggtcgccaa tcgaatcacg
     6721 acggaggacg cttctcccat cgcggcagcc tgcggattga acccgcacgt cgacgaggtg
     6781 ctcatgcgcg ccctcgcgag aaaccctgcg gatcgctacg agagcgctcg cgagtttggc
     6841 tacgcactcg ccgaagggct cgaggtcgct ccccgcacgg ccatggcgac
//
LOCUS       DASTKS010001330         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_437155, whole genome shotgun sequence.
ACCESSION   DASTKS010001330 DASTKS010000000
VERSION     DASTKS010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437155"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ35_28535"
     CDS             <1..205
                     /locus_tag="VFQ35_28535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934699.1"
                     /translation="RHVDRVIRVSDDGKLYENIGANETAGGGWKREWTPFASAKLLGF
                     VEYPGIPESHTKDAEGLASEHVG"
     gene            306..530
                     /locus_tag="VFQ35_28540"
     CDS             306..530
                     /locus_tag="VFQ35_28540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934700.1"
                     /translation="MKSSPAIDIRALLLIALDEQPPPNLPERVMARVALLTTVLEFGR
                     LLGAAPFDWLLRRRGGSGPDDDDKGSGAGG"
     gene            541..1392
                     /locus_tag="VFQ35_28545"
     CDS             541..1392
                     /locus_tag="VFQ35_28545"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934701.1"
                     /translation="MTSHPLYERLVKKPLELAWTEAVARLPTLGSAIAVGVALWLLAR
                     VVRALAARLLKMSRLDRVVEDTWFGRILSGLGDGFTPSKAIASLLYVAILMLALAACA
                     DILGLSAVRNAMMAALGYVPRIASALCVVAVGGYVAKVARRAVESVMNELKSPYGGIA
                     ESTTEGLILLLTVTVAVNSLGADLSFITNNLAIIVAVLVVTAAFLFAWSMRRPAEEII
                     ANYYLRRLVRVGDRVTLANIEGVVERFTALGLLLKDEAGLEHFVPARHVLDGVQRSES
                     AGALRRK"
     gene            1399..3072
                     /locus_tag="VFQ35_28550"
     CDS             1399..3072
                     /locus_tag="VFQ35_28550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934702.1"
                     /translation="MEAEAAVSGFGAELSDAALVGLYRDGAGEFAFAELVRRHQIAVF
                     RLLLTLLGDADRAERACEQVFFDAARRLGELQAPDGFYSWVAGLARARAQKLEAESQK
                     SKPPERPRSPPKDPRAAVKRHVKDVLRTMSGDERVALVLADLEGDSFEAIAATLGTSV
                     PEAEALVTQAREKFVLALTEGEGASAPGESAASSTGSLPPGSVLGGRYRIERMLGKGG
                     MGAVYSATDLESGTTVALKTLLPAAAADASLRRRFEREAELLRRLAHPNFVRFLAYGN
                     APDEPTYVVMEFVDGTLMSQLLKLESRLPPQRALHIVRHLLTGLGFAHGLGVVHRDIK
                     PENVMLLKSPEDDDFAKILDLGIARAFSPEDLRRTQLTEKNEVFGTPTYMSPEQVRGE
                     AVDGRSDLYSVTALLFELLSAHPPFEAKSPMALFAMHLASEPPALFDVAPDLRVPSGM
                     QELLDRGLAKEKDERFESAAEYLKKVDALLGADWSHAGPPTPRAEPRHTPLARRETVK
                     RPAANAPLPQKSRAARARERLRRIPFSAAVALIALVVYAVYRGIRYLSE"
BASE COUNT          513 a         1023 c         1114 g          577 t
ORIGIN      
        1 acgccacgtc gatcgggtga tccgcgtttc ggacgacggc aagctctacg agaacatcgg
       61 cgcgaacgag accgccggcg gcggctggaa gcgcgagtgg acgccgtttg cctccgccaa
      121 attgctcggc ttcgtcgaat acccaggcat ccccgagtcg cacacgaagg acgcggaagg
      181 gctcgcgtcg gagcacgtcg gctgactggg tgtaggaaaa gctgcgctgg cggggtgcac
      241 acctgaagat gcgcgccgtt tgcaggcggg cggtgtaggg tgaagccgtt cgaggacagc
      301 cgagcatgaa atcgtcgcct gcgatcgaca ttcgagcgtt gttgctcatc gcgctcgatg
      361 agcaaccgcc gccgaacctt ccggagcggg tgatggcgcg ggtggcgctg ctgacgaccg
      421 tgctcgagtt cgggcgtctg ctcggagcgg cgccgttcga ttggctcttg cgacgccgtg
      481 gagggagcgg cccggatgac gatgacaaag gttctggggc tggcggctag cgcgccgtcg
      541 ttgacttcac atccgctcta cgagcgcctg gtcaagaagc cgctcgagct tgcttggacg
      601 gaggcggtcg ctcggttgcc cacgctgggt tcggccatcg cggtcggcgt cgcgctgtgg
      661 ttgctcgcgc gtgtcgtgcg ggcgctcgcg gcgcggctct tgaagatgtc gcgcctcgat
      721 cgcgtggtcg aagacacatg gtttggccgc attctctctg ggctgggcga cggctttacg
      781 ccgtcgaaag cgatcgcttc gttgctgtac gtcgcaatcc tgatgttggc gctcgctgcg
      841 tgcgccgaca ttttggggtt gagcgccgtt cgcaacgcga tgatggccgc gctcggctac
      901 gttccgcgga ttgcgtccgc gctgtgcgtc gtggccgtcg gcggctacgt cgccaaagtg
      961 gcacggcgag cggtcgaatc ggtgatgaac gagctcaagt ccccgtacgg cggcatcgcg
     1021 gagtcgacca ccgagggcct catcttgctg ctgacggtca cggtcgcggt gaactcgctc
     1081 ggcgcggact tgtcgttcat cacgaacaac ctcgccatca tcgtcgcggt gttggtcgtg
     1141 acggcagcgt tcctgttcgc gtggtcgatg cgccgcccgg cagaagaaat catcgcgaac
     1201 tactacctgc gccggctcgt gcgggtgggc gaccgcgtga cgctcgccaa catcgaaggg
     1261 gtggtcgagc gcttcacggc gctcggcttg cttctcaaag acgaggcagg gctcgagcac
     1321 ttcgtgccgg cgcgacacgt gctcgatggc gtgcaacgct cggagtccgc cggcgcgttg
     1381 cgcagaaaat agcagcgcgt ggaagcggaa gcggcggtca gcggtttcgg cgcggagctg
     1441 agtgacgcag cgctcgtcgg cttgtaccgc gacggcgcgg gcgagttcgc gttcgccgag
     1501 ctcgtgcgac gccaccagat cgccgtattt cgtctgctgc tcacccttct gggtgacgcc
     1561 gatcgcgccg agcgcgcctg tgagcaagtc ttcttcgacg cagcacggcg cctcggtgaa
     1621 ctgcaggcgc ccgatggctt ttacagttgg gtcgcagggc tcgcccgcgc tcgcgcgcaa
     1681 aagctcgaag ctgagtcgca gaagtccaaa cctcccgagc gaccgcgctc tccgccgaaa
     1741 gatccgcgtg cggccgtgaa gcgacacgtg aaagacgtgc tccgcaccat gagcggcgac
     1801 gagcgcgttg cgctggtgct cgcggatctg gagggtgact ccttcgaagc cattgccgcg
     1861 acgctgggaa cgagcgtgcc cgaagccgaa gccttggtga cgcaggcgcg agagaagttc
     1921 gtgctggccc tgacggaagg ggaaggagct tccgcgccgg gcgagtcggc tgcttcctcg
     1981 accggctcgc tgccccccgg ctccgtactg ggtggtcgct accgtatcga gcggatgctc
     2041 ggcaaaggcg gcatgggcgc tgtttacagc gcgaccgacc tcgaaagcgg cacgaccgtg
     2101 gcgctgaaaa cgctgctgcc tgcggccgcc gcggacgcat cgttgaggcg gcgcttcgag
     2161 cgtgaggcgg agctcttgcg gcgcctcgca caccccaatt tcgttcgctt cctggcttac
     2221 ggtaacgctc cggacgaacc aacttacgtc gtcatggagt tcgtggacgg cacgctgatg
     2281 agtcagctcc tcaagctcga gtcacgactg ccgccgcagc gcgctctgca catcgtgcgg
     2341 cacttgctga ccgggcttgg cttcgcccac gggctcggcg tcgtgcaccg tgacatcaag
     2401 cccgagaacg tgatgttgct gaagtccccc gaggacgacg acttcgccaa aatcctcgac
     2461 cttggcatcg cgcgcgcatt ctctccagaa gatctgcgtc gaactcagct gaccgagaaa
     2521 aacgaagtgt tcgggacacc gacttacatg tcccccgaac aggtgcgcgg cgaggctgtc
     2581 gacgggcgct cggacttgta ctcggtgacg gcgctcctgt tcgagctgct ctcggctcac
     2641 ccgcctttcg aagcgaagag ccccatggct ctcttcgcga tgcacttggc ttccgagccg
     2701 cccgcgctgt tcgacgtcgc gccagacctc cgcgtgccga gcggaatgca agagctgctc
     2761 gatcgcgggc tggcgaagga gaaggacgag cgcttcgagt cggcggcgga gtacctcaag
     2821 aaggtagacg cgctgctcgg cgcagactgg agtcatgcgg gtccgcccac gcctcgtgcg
     2881 gagcctcgtc acacaccgct cgcgcgccgg gagacggtca agcggccggc tgcaaacgcg
     2941 ccgctgccgc agaaatcgcg cgccgcgcgt gcgcgggagc gactacgacg catccccttt
     3001 tcggccgcgg tagcgctgat tgcgctggtc gtttacgccg tttaccgcgg gatccgctac
     3061 ctgagcgagt agggcaccga cgtgctcagg gtttcgggtg tcgcgccgag ctcgacgcgt
     3121 gcaatgcatt ggttccgcgt gaggctcaag cgtggcaaac tccacgggga tggcgagctc
     3181 gacgacgcgt atgcgcactg ggcccagcgt gcccgtcaag acgctcc
//
LOCUS       DASTKS010001331         5741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_661094, whole genome shotgun sequence.
ACCESSION   DASTKS010001331 DASTKS010000000
VERSION     DASTKS010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661094"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFQ35_28555"
     CDS             complement(<1..1244)
                     /locus_tag="VFQ35_28555"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934703.1"
                     /translation="MDNWVEFGRVLLAVFGADVRERRDAMTSLKRESSLGFTATVAFA
                     VSAALIAVGCGGHESKERTEVAVATKVEALSVPPPATCTDHTWNLLTTGLTSACVDGP
                     GGFKCWGLWSILDPVTTQTTANFVFPPTNPTMNVGGSTRIRAAASGYRHTCVAADNGV
                     WCFGDNAGGQLGNGTQVGSQTPVRVNADFGDRVVSLSSKAMHTCALLNNGEVWCWGEN
                     SSFACGVAPASAALTTPGKVDLGGRTAKAIATGSGFSCALSDAGEVLCWGSNASGKAG
                     VPGAATLATPTLLDTSGKRAVLLSAGLEHGCAVFDDQTSACWGLNQYGTLATGDRISK
                     PSPTPVNLNSPAGAPIQLDAGARHQCAVYQDGSLRCWGKNVCWDMSGNSNPNSAGGQL
                     GLGVADSDWRLTPQSVPLGSRKV"
     gene            complement(1531..3174)
                     /locus_tag="VFQ35_28560"
     CDS             complement(1531..3174)
                     /locus_tag="VFQ35_28560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934704.1"
                     /translation="MRPRKKALRSVGVLAATLALPSCSFLLDFDSLQKGEGSGGGGLG
                     GVGGASGGNGGGGASSGGTALGGATSGGHAGGDTMSGGAPSGGAGGDGGAGGGSAGAP
                     EAGAGGTGVSCDCVDADADPCTVARCVDRGRGPECIQQPIEGLMLERDYDPIVAERFA
                     QLGLVAGPNEFYLSALSTTGNGSNAIVYRFSDSSASVVPALDLGSIKVAGAPVSVPAL
                     AVDTSNGVRIHGFAALREAGAGTGARVWHFTLDSDFKVQARVPVSADYPVAVDLLAQT
                     QQPAALSVGGETWGAWINSDGTVGVTSVGVNPDILTFGTPGPVPSTVSLLATRTGRPA
                     VLSTVDTVGQAGGVFLHSEGGSAQLSECQSGLGAYSSSYAAATSFPGLWAMGWTKSGT
                     GFLTSEGSLGLCPQNAPCATDPSCDANEAANFVRNPAVAAQHIQGDAPGQMYYVEVLP
                     TLAPASSTKNFQAQLTAVLVGVDFGSSANLGGVQPPVTLGDPIVVASQNTTAAAGYRG
                     PDHAAAAIVADTAAIAWVEPTMDGADRVRVQRYKMCLPR"
     gene            complement(3177..3950)
                     /locus_tag="VFQ35_28565"
     CDS             complement(3177..3950)
                     /locus_tag="VFQ35_28565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934705.1"
                     /translation="MKSAVSGRSGARLGLLALCVALCLPRAALAQGDNADDLARRHFE
                     SGVAYLEESDYDNALRAFQKAYDLSKRPEILINIATVQERRADIDGAIVALRQYLEVA
                     PNGEHAETVRLRVQNLEKRRPAPVPPEPKPEVTPPPPPPPPPPPVVELRPNRVPAFIA
                     LGVGAAAGIGAFVTGIVAKNKYDDAKLSCSPHCTDSQLSSSRGFALTSTILTGAAVLG
                     AGVGVTLLVLGKPTESKSTAELVILPSVGGAAARASWRF"
     gene            4217..>5741
                     /gene="carB"
                     /locus_tag="VFQ35_28570"
     CDS             4217..>5741
                     /gene="carB"
                     /locus_tag="VFQ35_28570"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9934706.1"
                     /translation="MPRRNDINKILLIGSGPIVIGQACEFDYSGTQGAKALTSLGYDV
                     VLVNSNPATIMTDPELVRRTYIEPLTVDTLSAIIERERPDALLPTLGGQTALNLALEL
                     HGRGVLSKHGVRLIGAQVDAIRKAEDRELFKQAMARAGLEVARSGHAGSVEEAHEIVK
                     FTGYPVILRPSFTMGGAGGAVVYSPDEFETKVEWALSQSPTRQVLVEESLLGWKEYEL
                     EVIRDAKDNFIVICSIENIDPMGVHTGDSITCAPAMTLTDREYQRLRDAARAVMHEIG
                     VETGGSNVQFAVDPKTGRVIVIEMNPRVSRSSALASKATGYPIAKIAAKLAVGFTLDE
                     LENDITGTSAAFEPTIDYVVVKWPRFAFEKFPGADPRLGTQMKSVGEAMAIGRTFPEA
                     LQKAARSLETGKSGLVSLVDRIDYRVLAREPETRRDLTRDAPPAARPKPTLPPPGAHE
                     LEAALVKLIPIATADRLFYVTDALRLGVSEERVNELTGIDPWFVAQIGRIVAQEKVIK
                     "
BASE COUNT         1026 a         1942 c         1869 g          904 t
ORIGIN      
        1 acctttcggc tccccaaagg cacactctga ggagttaagc gccaatcgct gtccgcgacg
       61 cctagcccga gctgcccacc tgccgagttc gggttcgaat tgccgctcat gtcccagcaa
      121 acgttcttgc cccaacatcg cagtgagcca tcctggtaga cagcgcactg gtggcgagca
      181 cctgcgtcca actggatggg agcacccgcg gggctattca aattgacggg ggtcggcgac
      241 ggtttgctga tgcggtcgcc ggtcgctagc gttccgtact gattgaggcc ccagcacgcg
      301 ctggtttgat catcgaagac ggcgcagccg tgctcgaggc cagcggacag cagcacggcg
      361 cgtttgcccg acgtatcgag cagggtgggc gtcgcaagcg tcgccgcgcc gggaacgccg
      421 gcctttccgc tagcattcga gccccagcac aggacctctc ccgcgtcgga cagcgcacaa
      481 ctgaagccac tcccggtggc gatcgctttg gcggttcgac ccccgaggtc gaccttgccg
      541 ggcgtggtaa gagcagcgct cgcgggggcc actccgcacg caaagctcga gttctcaccc
      601 cagcaccaga cctccccatt gttgaggagc gcacacgtgt gcatcgcctt cgaggacagg
      661 ctgacgaccc tatcgccgaa gtcggcgttg acacgcaccg gagtttggct cccaacctga
      721 gtcccatttc caagctgacc gcccgcgttg tctccgaagc accagactcc gttgtcggcc
      781 gcaacgcacg tatgcctgta gccagatgcg gcggcgcgaa tccgcgtcga cccgccgacg
      841 ttcatcgttg ggttggtggg cggaaacacg aagtttgcag tggtttgggt ggtgaccggg
      901 tcgaggatcg accacagtcc ccagcacttg aaaccgcccg ggccatcgac acacgccgaa
      961 gtgagccccg tcgtgaggag attccaagtg tggtcggtac acgtcgccgg cggcggaacg
     1021 ctgagcgcct cgacctttgt cgcgacggcg acctcggtcc gctcttttga ttcgtgtcca
     1081 ccgcacccga ctgcgatgag cgccgcgctc acggcgaaag caaccgtcgc ggtgaaccca
     1141 agcgatgact cgcgcttcag cgaggtcatt gcgtcgcgtc gttcgcgaac gtccgctccg
     1201 aacaccgcca gaagcacacg cccaaattca acccagttat ccatctcgta ctccgcgcca
     1261 accggtcagg tctcagcccc aaggctgcct gcgcatccac caacgcgcgc tctctaggca
     1321 atcggcgtgc ctgggtgccg cacgcggcag ttcttcaaaa gcgcgcgaaa agctcgagat
     1381 ttgtttgcga acggcagagc aaagcagccc gagtgtccgc gaacatccgc aaacgttcac
     1441 gaacaggcga gagatgctag ggacgcgaat acgtctggcg gcgagacgct ggctcagcgc
     1501 tgaaacgctg acgttatcgg gttcgccggc tcagcgcggc agacacatct tgtagcgctg
     1561 cacgcggact cgatcggcgc cgtccatggt gggctcgacc caggcaatgg cagcggtgtc
     1621 ggcgacgatg gccgcggcgg cgtgatctgg accgcggtaa ccggcggcgg cggtcgtgtt
     1681 ttggctcgcg acgacgatgg ggtcaccgag cgttaccgga ggctgcacgc cgccgaggtt
     1741 cgcgctgctg ccgaagtcga cgccgaccaa cacggccgtg agttgcgcct ggaagttctt
     1801 ggtcgaagac gcaggggcga gcgtcggcag gacctcgacg tagtacatct ggcctggagc
     1861 atcgccctga atgtgctgcg ccgcgaccgc ggggttgcga acgaagttcg cggcctcgtt
     1921 ggcgtcgcac gacggatccg tcgcgcaggg cgcgttttgg ggacagagtc cgagggagcc
     1981 ttcgctcgtg aggaagcccg tccccgattt ggtccatccc atcgcccaca ggccgggaaa
     2041 gctcgtggcg gctgcgtagc tcgatgagta ggcgccgagg ccggactggc actcagagag
     2101 ctgcgccgag ccaccctcgg agtggaggaa gacaccgccg gcttgcccaa ccgtgtctac
     2161 ggtcgagagc acggcgggcc gccccgtgcg ggtcgcgaga agcgaaacgg tactggggac
     2221 gggtcctggc gtgccgaacg tgaggatgtc ggggttcaca ccgaccgagg tcacgcccac
     2281 agtgccgtcg ctgttgatcc acgcgcccca cgtttcgccc ccgacgctga gcgctgcggg
     2341 ttgctgcgtc tgggcgagca ggtcgaccgc gacggggtaa tcggcgctga cgggaacgcg
     2401 ggcttgaact ttgaagtcgg agtcgagggt gaagtgccag acgcgcgcgc ccgtgccggc
     2461 gccggcctcg cgcagcgcgg cgaagccatg aatgcgcacg ccgttgctcg tatcgacggc
     2521 gagcgcaggc acgctgaccg gagctccagc cactttgatc gaaccgagat cgagtgcggg
     2581 aacgacgctc gcgctcgagt cactgaagcg atacacgatc gcgttcgagc cgttgccggt
     2641 cgtagatagc gccgagaggt agaattcgtt cggtcccgcg acgagcccga gctgtgcaaa
     2701 gcgttccgcg acgatcggat cgtagtcgcg ttccagcatc agaccttcga tcggctgctg
     2761 aatacactcg ggacctcgac cgcgatcgac gcagcgagca acggtgcacg gatcagcgtc
     2821 ggcgtcgacg caatcacagc tgactccggt acctccggct cccgcttccg gcgcacccgc
     2881 ggagcctcca ccggccccac cgtcgccgcc agctccgccg ctcggcgcgc cgccggacat
     2941 ggtgtcgccg ccggcgtgcc cacctgacgt cgccccgcca agggcagtgc ccccggagct
     3001 cgcaccaccg ccaccgttgc cgccgctcgc tccgccaacg ccgccgaggc cgccaccgcc
     3061 gctgccctcg cctttctgga gcgagtcgaa atcgagcagg aacgagcagc tcggaagagc
     3121 gagcgtggct gccagcactc cgaccgagcg cagcgctttc ttacgcggac gcactctcaa
     3181 aacctccagc tagcgcgagc ggcggcgcca ccgacactcg gaaggatcac gagctcggcc
     3241 gtcgacttcg actcggtggg cttccccagc accagcaggg tgaccccgac tccagcgccg
     3301 agaacggcag caccggtgag gatcgtactc gtgagcgcga acccacgact gctcgagagc
     3361 tgcgagtcgg tacagtgcgg gctgcaactc agcttcgcgt cgtcgtactt gttcttggcc
     3421 acgatgcccg tcacgaaagc gccaatgccg gcggctgcgc ccactccgag cgcgatgaac
     3481 gcgggaacgc gatttggccg cagctcgacc acgggcggcg gcggtggtgg aggaggcggc
     3541 ggaggcgtga cctccggctt gggctcgggc ggcaccggcg ccggccgccg cttttcgagg
     3601 ttctgcacgc gcagccgtac agtttcggcg tgctcgccgt tcggagcgac ctccaagtat
     3661 tgtcggaggg cgacgatggc tccgtcgatg tcggcccggc gctcctgcac cgttgcgata
     3721 ttgattaaga tttccgggcg cttggagaga tcgtacgctt tctgaaaggc cctgagcgcg
     3781 ttgtcgtagt cgctctcctc caagtaggcg acgccggatt cgaagtggcg gcgagccagg
     3841 tcgtcggcgt tgtcaccttg cgcgagcgcc gcccgcggca aacacaacgc gacgcagagg
     3901 gcgagcaagc ccaaacgggc cccacttcgg cctgaaaccg cagacttcat cgctgtgcaa
     3961 ggcaactggg agcgatcccc agccggcgct actgccataa aaacgctcat atcatgccaa
     4021 cgtctacgcg ctaagcgctc gacacgttcc cgccgctttc tgttcacgct gcaagcgccg
     4081 tgagcaccgc agcggtgggc cccgtggagg aggcggcgac gccggccccg agaccagccc
     4141 accggaagcg ctcggttctc gtggaaagtg caaaaccagc gagaccgtcg aacgtgtgtg
     4201 ctaaggcgct cgcgggatgc cgcgccgtaa cgacatcaac aagatcctcc tcatcggctc
     4261 cgggccgatc gtcatcgggc aagcctgcga gttcgactac tccggcacac aaggagccaa
     4321 ggcgctcacg agccttggct acgacgtcgt gctggtgaac tcgaacccag cgacgatcat
     4381 gaccgacccc gagctcgtgc gtcgcacgta catcgagccg ctcacggtcg atacgctttc
     4441 cgcaatcatc gagcgggagc gccccgacgc gctcttgccg acgctcggcg gtcagacagc
     4501 gctgaacctc gccctcgagc tgcacggccg cggggtgctc tcgaagcacg gcgtccgact
     4561 catcggcgct caggtcgacg cgatccgcaa agcggaagat cgggagctct tcaagcaggc
     4621 gatggcgcgc gcgggcctcg aagtggcgcg ctcgggtcac gccggttcgg tcgaagaggc
     4681 gcacgaaatc gtgaagttca ccggctatcc ggtgatcctc cgaccgagct tcacgatggg
     4741 cggcgctggc ggcgcggtcg tctacagccc cgacgagttc gagaccaagg tggagtgggc
     4801 gctctcgcaa tcgccgacgc gccaggtgct cgtcgaagag agcttgctcg gctggaaaga
     4861 gtacgagctc gaggtcatcc gcgacgcgaa ggacaacttc atcgtcatct gctcgatcga
     4921 gaacatcgac ccgatgggcg tgcacaccgg tgactcgatc acctgtgctc ccgcaatgac
     4981 gctgaccgat cgagagtacc agcggctgcg cgacgcggcc cgcgcggtca tgcacgagat
     5041 cggtgtcgag accggcggct cgaacgtgca gttcgcggtc gacccgaaga cggggcgcgt
     5101 catcgtcatc gagatgaacc cgcgcgtgtc gcgctcgagc gctctcgcct cgaaagcgac
     5161 gggctacccc atcgcgaaga tcgcggccaa gctcgcggtg ggcttcacgc tcgacgagct
     5221 cgagaacgac atcaccggca cgagcgccgc gttcgaaccg accatcgact acgtggtcgt
     5281 gaagtggccg cgcttcgcgt tcgaaaagtt ccccggtgcc gacccacggc tcggcacgca
     5341 aatgaagagc gtcggcgaag caatggccat cgggcgcacc ttccccgaag cgctgcagaa
     5401 agccgcgcgc tcgctcgaga cgggcaagag cggcctcgtg agcctcgtcg atcgcatcga
     5461 ctaccgcgtg ctcgcgcgcg aacccgagac acgccgcgat ctcacccgcg acgcgccgcc
     5521 cgctgcgcgc cccaagccca cgcttccgcc gcccggcgcc catgagctcg aggccgcgct
     5581 cgtcaagctc atcccgatcg cgaccgcgga tcgcttgttc tacgtgaccg acgcgctgcg
     5641 cctcggggtc tccgaagagc gcgtgaacga gctcactggg atcgatccct ggttcgtcgc
     5701 gcagatcggc cgcatcgtcg cgcaagagaa ggtcatcaag a
//
LOCUS       DASTKS010001332         6149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_215757, whole genome shotgun sequence.
ACCESSION   DASTKS010001332 DASTKS010000000
VERSION     DASTKS010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215757"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="VFQ35_28575"
     CDS             complement(<1..1228)
                     /locus_tag="VFQ35_28575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9934707.1"
                     /translation="MTIVSTQAVHKSYGPRVILEAADLAVQKNERIGVVGRNGAGKST
                     LARIIAGIEQPDTGTVSRMRGASIEYLSQTPSFEGDPTALGALLSAIAGWSEAVKTHE
                     RATEQLALGQGNQKVLLEEQQHAAEAVERLGGWQLQHQAEALLGHLGVTRPDARVAIM
                     SGGEQRRVALARLLISRPDLAILDEPTNHLDAETVEWLENYLIDEHQGAILLITHDRY
                     LLDRVAERTLEVADGLVFSYAGGYELYLEQKAERAEHAARTEQNRQNFLRRELEWLRR
                     QPKARTTKQKARIERAESAKASIPLKEQTTAKFSLETSRAGKTILEAVGLSLEIGGRE
                     LVSGLDLFLTQGEIIGVVGRNGAGKTTLLRAIVGEHPVKSGTLRIGQNTKIGYFDQKR
                     SGLDDEKSVFDNVAGDD"
     gene            1409..2539
                     /locus_tag="VFQ35_28580"
     CDS             1409..2539
                     /locus_tag="VFQ35_28580"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934708.1"
                     /translation="MKATPFLHPSAARAGALLLVVSVVGCSSTSEPSPQGSGGNANGG
                     SANSGGTSTGSGGATSGGVTTGGAANGGHDSSGGSANGGTSSNSGGASGGSASGGRSA
                     SGGSTSGGSTSGGSASGGNSTSGGAASGGSVASGGSSSGGGSTSSSGGVGGQPGGAGC
                     PSGVTWCSGFEDSGLPQGAVYKLNGDPATPWTHDFEIDTTQHKSGKSSLRVKSASEAG
                     GAYKMLAVPSGGSAFWVRFYIRSDADLGASAHNVYAQASGSDEPNDASSVEFAEDVGL
                     AFNSHDDVRWPDGYGRTTSGGTKPYTLPKDTWHCIEVSFDGQGKQQKLFINGMQQIDA
                     TMFPKSAYTFKNFKFGYNSLNGTQRKVWYDDVAVGPSRINCQ"
     gene            complement(2601..3392)
                     /locus_tag="VFQ35_28585"
     CDS             complement(2601..3392)
                     /locus_tag="VFQ35_28585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9934709.1"
                     /translation="MSSLLDSMMDAARLAGAGLRNDFARARELAVEEKGASDFVSNAD
                     LASQALIERELRRNFPEAVLVLEEERSSHTLSGALRFYVDPLDGTTNFLHAIPHFSIS
                     IAGERDGELVAGVVYDVVKDELFAGERGGGAWLNGRRLSVSSESRLDRAVVGTGVPHR
                     GRLAHAEYLSVLPRIMADFAGIRRFGSAALDLAYVAAGRFDAFFESHLSPWDIAAGVV
                     LVREAGGVVLKPNGSAFELAAGHVLACNEALRAPMCERLSGLIQQ"
     gene            3610..4332
                     /locus_tag="VFQ35_28590"
     CDS             3610..4332
                     /locus_tag="VFQ35_28590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934710.1"
                     /translation="MKIRLGLLALPLALSLSNGLAWADDDDRPQREDPWEYDDAKKKA
                     REQEEEDEASEDAFANAGQLVLSAERLVGVSRTAIETKVGGVKHKDSVNRANLLLNQD
                     GNVYGYSHPRVAFDYFVIDGLSLGGAVGFSVNSGDAANRELILAPRVGYAYMFSDTVG
                     VWPRLGGTYQYQKTPEGVTGLLAISFEANLVIVPTEHVAFTIAPTIDASLVGKFNPVG
                     PEKKSDYKQEEIGLQTGLSVFF"
     gene            complement(4457..>6149)
                     /locus_tag="VFQ35_28595"
     CDS             complement(4457..>6149)
                     /locus_tag="VFQ35_28595"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HET9934711.1"
                     /translation="GDGSGCSKTCTQEPTCRDAAGVTTACTARCGDGNIDPSEECDDS
                     NQAKGDGCDDKCKKEGGFDCKPVENSDLTDCTMTAGKCLVLPITFRDFDGQQASSGHP
                     DFFYYSSTTTCVPNASGVNPAAGAPLNGTCPANDSTALCSGLVKATLGTDGKPAVAKP
                     GMSTCACRFTDWDGTGLLNTVTNAMNCNDSGGNQHKTYSANVSVIQSDASFKQWYSDS
                     DKSTKVVSTLELAQVGASNQFQFSSSNGRTVYDDLHDIFMGTGVTSLTSGFFPLETSN
                     RPKVCNLWPYWLSNSACVAKDGATPSQQWDPRGSYTAMMAGTGGPVKPVTGMMRNFYF
                     TSEVRYLFRYAGGETLSFFGDDDVWVFINGHLVLDLGAPHERMKGTVTLTATGATYAI
                     TGRDNATGMDIAITGGSGNVSNLGMEVGKTYEIAIFHADRHPRESNYQLTLSGYKTTV
                     TQCTPTCGDNVATAGEECDLGDKNNDDEYGGCTKACKFGPFCGDASKNGPEECDKGKD
                     NGGSYGQKDGCTLGCKKTHFCGDGIVDAAQGEECDAGSANSATGSCTDMCKYKPK"
BASE COUNT         1098 a         2000 c         1945 g         1106 t
ORIGIN      
        1 cgtcgtcccc cgcgacgttg tcgaagaccg atttttcgtc gtcgaggccg ctacgcttct
       61 gatcgaagta gccaatcttg gtgttttgcc cgatgcggag cgtgcccgac ttgacgggat
      121 gctcgccgac gatggcgcgc agcaacgtcg tcttaccggc gccgtttcgg ccgaccaccc
      181 cgatgatttc gccttgagtc aggaacaagt cgagccccga cacgagctcg cgtcccccga
      241 tttcgagcga cagcccgacc gcctcgagga tcgtctttcc ggcgcggctc gtctcgagag
      301 aaaacttcgc cgtcgtctgc tccttgagcg ggatgctcgc cttggcggac tcagcgcgct
      361 cgatgcgagc cttttgcttg gtggtgcgcg ctttcggctg ccgcctcaac cactcgagct
      421 cgcggcgcag gaagttttgg cggttctgct cggtgcgcgc cgcatgttcg gcccgctcgg
      481 ccttttgctc gaggtagagc tcgtagccgc cggcgtacga gaagacgagc ccatccgcga
      541 cttcgagcgt gcgttcggcg acgcggtcga gcaggtagcg gtcgtgcgtg atgagcaaga
      601 tcgcgccttg atgctcatcg atgagatagt tctcgagcca ctcgaccgtt tcggcgtcga
      661 gatggttcgt cggctcgtcc aggatggcga ggtcgggtct cgaaatgagt agccgcgcca
      721 acgcaacacg gcgctgttca ccgccgctca tgatcgcgac gcgcgcgtcg ggccgagtca
      781 cgcccaaatg gccgagcagc gcctccgctt ggtgctggag ctgccagcct cccaaccgtt
      841 ccacggcttc agcagcgtgc tgctgctcct ccagtagaac tttttgattt ccctgtccca
      901 gcgcgagctg ctcggtcgct cgctcgtgcg ttttgaccgc ttcgctccaa ccagcgatcg
      961 cgctgagcag cgcgccgagc gccgtcggat cgccctcgaa cgatggcgtt tgcgagaggt
     1021 actcgatgct ggcgccgcgc atccgcgaca cggttccggt gtccggctgc tcgatgccgg
     1081 caatgatccg cgcgagcgtg ctctttccag cgccgtttcg accgacgact ccaatacgct
     1141 cgttcttttg caccgcgaga tcggcggcct cgaggatgac gcgaggccca tatgacttgt
     1201 ggacggcttg ggtcgaaacg atggtcacgc gacgccaaca ttacacgcga acctcggtgg
     1261 tcgcccgcct cgcgcggtga atcgccgcgt tgggcgagag gaccgggcaa aaaccacgtc
     1321 gggcacgaga ccacgcaatg cgactgctcg gcggttcgga ttgcattgga ccaaccgcga
     1381 gtcagcggtc acgctggcgg acgactgaat gaaagcgacg ccctttctcc acccttcggc
     1441 cgcgcgcgcg ggcgcgcttc tcttggtcgt gagcgtcgtc ggctgctctt cgaccagtga
     1501 gcccagcccg cagggcagcg gtggaaacgc gaacggcggc tcggcgaatt cgggcggcac
     1561 gagcacgggc tccggtggtg ccacgagcgg aggagtgacc accggcggcg ctgcgaacgg
     1621 cggccacgac tcgagcgggg gaagcgcgaa tggcggcacc agctcgaaca gcggaggcgc
     1681 gagcggaggc agtgcaagcg gtggccgctc cgcatccggc ggtagcactt ccggcggtag
     1741 cacttccggc ggcagtgctt cgggcgggaa ctccacttcc ggcggggccg cttcaggcgg
     1801 ttcggtggcg agcggcggct cgagtagcgg cggcgggagc acctcctcca gtggtggcgt
     1861 gggaggacaa ccgggcggcg ccggctgtcc ctcgggcgtc acgtggtgct ccggttttga
     1921 agactcgggg cttccccagg gcgcggtcta caaactcaac ggcgatccag cgacgccgtg
     1981 gactcacgat ttcgaaatcg acaccaccca gcacaagtca ggaaagtcgt cgctgcgcgt
     2041 gaagagcgcg tccgaggcgg gcggcgctta caagatgctg gccgtcccct ccggcggctc
     2101 ggctttctgg gtgcgattct acattcgcag cgacgcggat ctcggggcga gcgcgcacaa
     2161 tgtctacgct caagcttcgg gcagcgacga gccgaacgat gcgagcagcg tcgagttcgc
     2221 cgaagacgtc gggctcgcat tcaactcgca cgacgacgtg cgttggcccg acgggtacgg
     2281 aaggacgacc agcggcggaa ccaagcccta tacgctgccg aaggacactt ggcattgcat
     2341 cgaggtctcg ttcgacggcc agggcaagca gcagaagctc ttcatcaacg gtatgcagca
     2401 gatcgacgcg accatgttcc cgaagagtgc gtacaccttc aagaacttca agttcgggta
     2461 caactcgctc aacggcactc agcgcaaggt ctggtacgac gacgtcgcgg tcgggccctc
     2521 gcgcatcaat tgccagtgag cgcaccgagc acgctctggt gattgggccg ggagcaaagc
     2581 ttgccgcgcc gggccgtcga ctactgctgg atcaaacccg agagccgctc gcacatgggc
     2641 gctcggagcg cttcgttgca cgcgagcacg tgaccggcgg cgagttcgaa tgcgctgccg
     2701 ttcggcttca gcacgacgcc tcccgcctcc cgcaccaaaa cgacgcccgc ggcgatatcc
     2761 cagggtgaca agtggctttc gaagaaagcg tcgaaacgcc cagccgcgac gtaagccaaa
     2821 tcgagcgccg ccgagccgaa tctacgaatc ccggcgaagt cggccatgat ccgcggcaac
     2881 acggacagat attcggcatg cgccagccga ccccgatgcg gcacgccagt accgaccacg
     2941 gcgcgatcca agcgcgactc cgaagagacg ctcaggcgcc gcccgttgag ccacgccccg
     3001 ccccctcgtt cccccgcaaa gagctcgtct ttgacgacgt cgtacacgac acccgccacg
     3061 agctcgccat cgcgctcacc agcgattgaa atggaaaagt gggggatcgc gtggaggaag
     3121 ttcgtggtcc catccaaggg atcgacgtaa aagcgaagcg cgccggacag cgtgtggctc
     3181 gaacgctcct cttcgagcac gagcacggcc tcgggaaaat tgcggcgcag ctcccgttcg
     3241 atgagagctt gcgacgcaag atcggcgttg gagacgaagt cgctcgcgcc cttctcttcg
     3301 accgcgagct cgcgggcgcg agcaaagtcg tttcgaagcc ccgcaccagc gaggcgagcg
     3361 gcatccatca tcgaatcgag aagtgagctc aagggatctc cgaaaagtgc ctcggcatac
     3421 gagtccgagg tgtgcgcaac ctaccatgta cggaaacgtg gggtgtgttc ccagcgggaa
     3481 atgcaccaaa ggcaaatcca agtagtccgc tgaggaaaca tgtgtttgaa acgacccacg
     3541 acgcgcgaca ctcggggctg ctacgccagc cacgaaccgt cgcagcgcgc acaaaatctg
     3601 gagctaacga tgaagattcg actcggactg ctggcgctgc cgctcgctct gtcgctctcg
     3661 aatgggctcg cttgggcaga cgacgacgac cgtccgcagc gcgaagaccc gtgggaatac
     3721 gacgacgcca agaagaaagc gcgggagcag gaagaggagg acgaggcgtc ggaggatgcc
     3781 ttcgccaacg ctgggcagct cgtattgtca gccgagcgcc tggtcggagt gagccgcacc
     3841 gccatcgaaa ccaaggtcgg aggcgtcaaa cacaaggact cggtcaaccg cgcgaacctg
     3901 ctcttgaatc aagacggcaa cgtgtacggc tactcgcacc ctcgcgtcgc cttcgactac
     3961 ttcgtcatcg acggcctcag cctcggtggc gcggtcggct tcagcgtcaa ttccggcgac
     4021 gccgcgaacc gggagctcat cctcgcgccc cgcgtcggct acgcctatat gtttagcgac
     4081 accgtcggcg tctggccgcg gttgggcggc acataccaat accaaaagac gccggagggc
     4141 gtgactggtc tcctcgcgat cagcttcgaa gcgaacctgg tcatcgtccc caccgaacac
     4201 gtcgccttca ccatcgcacc aaccatcgac gcaagcctcg tcggcaagtt caatccggtc
     4261 ggcccagaga agaagagcga ctacaagcaa gaggaaatcg gccttcagac tggcctgtcc
     4321 gtcttcttct gagcgccgga aagggcacgc gtcgctccgt ctgagcgagt gttggcccca
     4381 caaaacgaaa ccgggacagc tcgtgagaac tgtcccggcg tcgactagcc caggcgcggc
     4441 gccctaggct gctcgctcac ttcggcttgt acttgcacat gtcggtgcag ctgccggtcg
     4501 cgctgttggc agacccggcg tcgcattcct cgccttgcgc cgcatcgacg atgccgtcgc
     4561 cgcagaagtg cgtcttcttg cagcccagcg tgcagccgtc cttctgaccg tagctgccac
     4621 cgttgtcctt gcccttgtcg cactcttccg gaccgttctt gctggcgtcg ccgcagaacg
     4681 ggccgaactt gcacgccttg gtgcagccgc cgtactcgtc gtcgttgttc ttgtcgccga
     4741 ggtcgcactc ttcgccggcg gtcgcaacgt tgtcgccgca ggtcggagtg cattgggtca
     4801 cggtcgtctt gtaccctgac agagtcagct ggtagttcga ctcgcgggga tggcggtcag
     4861 cgtggaagat ggcgatttcg tacgtcttgc caacctccat cccgaggttt gacacgttgc
     4921 cactgccgcc ggtgatggcg atgtccatac ctgtcgcgtt gtctcgccca gtgatcgcat
     4981 aggttgcgcc agtggcagtc aacgtcaccg ttcccttcat acgctcgtga ggcgcaccca
     5041 ggtcgagtac caggtgaccg ttgatgaaca cccacacgtc gtcgtcgccg aagaacgaga
     5101 gcgtctcacc gcccgcgtag cggaacaggt agcgaacctc tgacgtgaag tagaagttcc
     5161 gcatcatgcc ggtgaccggc ttaaccgggc cgccggtgcc ggccatcatc gccgtgtacg
     5221 acccgcgcgg atcccactgc tgactcggag tcgcgccgtc ctttgccacg cacgccgagt
     5281 tgctgagcca gtatggccat aggttgcaga ccttcggccg gttgctcgtc tcgagcggga
     5341 agaagccgct ggtcaggctg gttacgcccg tccccatgaa gatatcgtgg agatcgtcgt
     5401 agaccgtgcg gccattgctg ctgctgaact ggaactgatt gctcgcaccc acctgcgcga
     5461 gctcgagcgt cgaaacgact ttggtgctct tgtccgagtc cgagtaccac tgcttgaagc
     5521 tcgcatcgct ctgaatgacc gacacgttgg cagagtaggt cttgtgctga ttaccgccgc
     5581 tgtcgttgca gttcatggcg ttcgtcaccg tgttgagcag gccagtgcca tcccaatcgg
     5641 tgaagcggca tgcacaggtg gacatccccg gcttcgcgac cgccggcttg ccatccgtgc
     5701 cgagcgtcgc cttcacgagc ccgctgcaca gtgcggtcga atcgttcgcc gggcacgtcc
     5761 cgttcaacgg agcccccgcc gcggggttca caccgctggc gttcggcacg cacgtggtcg
     5821 tcgatgagta gtagaagaaa tcggggtgac cgctcgatgc ctgttgaccg tcgaagtcgc
     5881 ggaacgtgat cggcaggacg aggcacttgc ccgccgtcat cgtgcagtca gtgaggtccg
     5941 agttctcgac cggcttgcag tcgaacccgc cctctttttt gcacttgtcg tcgcaaccgt
     6001 cgcccttggc ctggttgcta tcgtcacact cttcgctggg gtcgatgttg ccatcgccgc
     6061 agcgcgcagt gcacgcggtc gtgacgcccg ccgcatcacg gcaagtcggc tcttgcgtgc
     6121 acgtcttcga gcagccggag ccgtcaccg
//
LOCUS       DASTKS010001333         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1333328, whole genome shotgun sequence.
ACCESSION   DASTKS010001333 DASTKS010000000
VERSION     DASTKS010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333328"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFQ35_28600"
     CDS             complement(<1..327)
                     /locus_tag="VFQ35_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET9934712.1"
                     /translation="MSYLAPSEFVTRMVDAGESKIFMSARDTLIRACMAGALLSLAAA
                     FAVSVTVQTGSPLVGAMLFPVGFCILYLLGFDLLTGVFVLSPLALVDKRRGVTLGGVL
                     RNWGLVF"
     gene            complement(589..1368)
                     /locus_tag="VFQ35_28605"
     CDS             complement(589..1368)
                     /locus_tag="VFQ35_28605"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9934713.1"
                     /translation="MTQTSNPSIPSTPLAGKVALVTGGTSGIGRVTAELFRAAGARVV
                     LTGQDADRLESTRRELPADVVVLRADARSVPDAARVADEIRSRFGRLDVAFLNAGIAQ
                     LRPFEAVDEAHYDEHVDVNVKGVVFTLLKVLPLLGHGASVMVNTSLAAHKGTANMSMY
                     SLSKGAVSSLVRSLAVELAGRGIRVNAISPATIKTPIQVKFGLPPAVQMQVATDLCAR
                     IPLGRFGEAAEVAQAALFLASDASSYITGAELPVDGGLATS"
     gene            complement(1451..1984)
                     /locus_tag="VFQ35_28610"
     CDS             complement(1451..1984)
                     /locus_tag="VFQ35_28610"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET9934714.1"
                     /translation="MSEFWKRGYDATGLANLERATQLGRQSLYGAFGNKRALFSQVVE
                     HYFARVLKPAIVDVLDAPGSGRANIERVLAQWAETAGSAEFNGCLVGNAMAELGLRDR
                     ELSVLLQRKLDLLEAAFGRALRRAKRAGEVRADLDVRAAARTLLTMAQGLAVVARVQR
                     DSAFVRDVITVARQSLD"
BASE COUNT          424 a          704 c          655 g          333 t
ORIGIN      
        1 gaacaccaat ccccagtttc gcagcacccc gccgagcgtc acgccgcggc gcttgtccac
       61 gagcgcgagt ggactcaaca cgaacacacc ggtgagcaga tcgaacccca gcaagtagag
      121 gatgcagaag ccgactggaa agagcatggc ccccacgagc ggcgagccag tctgcaccgt
      181 caccgacacg gcaaacgccg ccgcgagcga cagcaaagcg ccggccatgc aagctcggat
      241 caacgtgtcc cgagcggaca tgaagatctt ggactcaccg gcatcgacca ttctcgtaac
      301 gaactcggac ggagccagat atgacatcgc gctgctctcc tcatcgatcg cgcgcagcat
      361 tgaattcatg ccgcttttcg caagcgcccc tcatgatccg cacgatgtgt gaatagtatt
      421 ttcccgctgc cgtcgcgaga ggcatgatgt ccccgggccg ccatcgtcgg ccgcgcaact
      481 gcggcttctc ggacggcgaa ttgcgcgtcg agagctccgc gtcggccgtc acatccgaaa
      541 aaggatgatg acggcgcgac gcgtgcccgc aacatgcgac gcccgagcct aggacgtcgc
      601 gagccctcca tctaccggga gctccgctcc cgtgatgtag gaagacgcgt cgctcgcgag
      661 aaagagcgca gcctgcgcta cctcggcggc ttcgccgaag cggccgagcg ggatgcgcgc
      721 gcacaaatcc gtcgcgactt gcatctggac cgcgggcgga agcccgaact tcacttgaat
      781 cggggttttg atggtggctg gggagatggc gttcacgcga ataccacggc ctgcgagctc
      841 gaccgcgagc gagcgcacca aagacgacac cgcgcccttg gagagcgagt acatgctcat
      901 gtttgccgtg cctttgtgtg ccgcgagcga tgtattcacc atcactgaag cgccatgacc
      961 gagcaggggc agaactttca gcaacgtgaa cacgacgcct ttcacgttga cgtccacgtg
     1021 ctcgtcatag tgtgcctcgt cgacggcctc gaacggacgc agctgcgcaa taccagcgtt
     1081 caagaacgcg acgtcgagcc gcccgaaacg cgagcggatt tcgtctgcga ctcgcgccgc
     1141 gtcgggcacc gagcgcgcat cggcacgcag gacgacgacg tccgccggca gctcgcgccg
     1201 agtggattcg aggcggtcag cgtcctggcc cgtcaaaacc acacgcgcgc cggcagcgcg
     1261 aaagagctcg gccgtgacgc gaccaatccc gctcgtgccg ccggtaacga gcgcaacttt
     1321 tcccgcgagc ggagtagacg gaatggatgg attcgaagtc tgagtcatgg gcattctcct
     1381 gggtcaatta tttaccgatc ggtccaaaat tccgcgaaca gcccgcggtg cggcggatga
     1441 aagcgccgag tcagtcgagt gattggcgag cgacggtgat tacgtcgcga acgaacgcac
     1501 tgtcgcgctg gacgcgggcg acgaccgcga gcccctgcgc catcgtcaac agcgtgcgcg
     1561 cggcggcgcg aacgtcgaga tccgcacgca cttcgccggc gcgcttggca cgacgcaatg
     1621 cgcgaccgaa agccgcctcg agcaggtcga gcttgcgctg caacagcacc gaaagctccc
     1681 gatcgcgcaa gccgagctcc gccatggcat tgccgaccag gcagccattg aattccgccg
     1741 aaccggccgt ctctgcccat tgggcgagca cgcgctcgat gttggcgcga ccggagccag
     1801 gcgcgtcgag cacgtcgacg atggctggct tcagcacgcg cgcgaagtaa tgctcgacga
     1861 cctgggagaa gagagctcgc ttgttcccga acgcgccgta gaggctttgc cggccgagct
     1921 gcgtggcgcg ctccagattg gcgagccccg tcgcgtcgta accgcgcttc cagaactcgc
     1981 tcatcgcgcg atccagcacc tgctccggct cgaatctccg cggcctgccc gccatcgctc
     2041 cgaccatgcc gcattttgac ctactggtca ataatgggta tctcgcgtaa gatgacgacg
     2101 cgagggcaaa tcttcg
//
LOCUS       DASTKS010001334         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1779395, whole genome shotgun sequence.
ACCESSION   DASTKS010001334 DASTKS010000000
VERSION     DASTKS010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779395"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VFQ35_28615"
     CDS             complement(<1..1858)
                     /locus_tag="VFQ35_28615"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HET9934715.1"
                     /translation="MLRPSLRQRVLGASGTKSLFRSAVAVIAFELGCSLIAPLEDVPE
                     PNGTATSGRGGDAGALSAGGTSVGNSGGSRFASGGATTIGDGGSQTGGDSGASGGATN
                     GGAPSSAKPDAGHGGQDNARTGGAPGTRGASDGGSSAASSGGSAGRGGVAAVGGGMAG
                     ESGTGGGVATGGGNGAMMALECEDEAQYFGMGSDSMRCTTPALWKFDHPVPYESISDW
                     SESEREQLQHALTAWTLAAPQIISFFELEPNKDVRPRLRFVAGDRCWAPFGQTLVNGV
                     QEVSLRGCDEATTQRELGRVLGMLNEQQRADRDRYLAVREPQLCSDPEPTISRRHFSE
                     IVSHCAPEGPPLAQQTIGPYDFDSIMQAPSRAPTGGTCTEADTSGCDLVKRSGNAFVP
                     GPTPTPLAGGGFSYISPLDAASFIERYHAAHSKWSAFQPMVRADPDPTQPLDTRLTSE
                     VRLVGSPAIARWGTSGLIALVRGDDRHLYYKVYASDDDTWSVGDWTDLGGDFISDPAA
                     VSRKEHSLDFVAVKANGYVYHGSYEDGVWSAATESLGRPNRDGVSAPAIAAWDSDRLD
                     VFIRSGRTLYQKSWQEAGWEAWKDVSSSLQIQGKPSAASWGPNRIDVVAVSID"
     gene            complement(2307..3221)
                     /locus_tag="VFQ35_28620"
     CDS             complement(2307..3221)
                     /locus_tag="VFQ35_28620"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET9934716.1"
                     /translation="MLPFDRRRFLFVTGKGGVGKTTVSAALARGLAERGKRVLLCTSG
                     AKEGLSPLLGVPRLGTSIELLAPNLWGVLLTSEVALREYGVLVLHSQTVVNALFDNKY
                     VSGLFNGAPGLKEWALLGKAWYHAIETLPNGALRFDVVVFDAPATGHSLDMLRVPKVI
                     LAAAPPGRLRTDAERAIAFLRDPASSGVVLATLPEEMPTNETLELARAVREELELPIV
                     EVVANSCLEELFTDAEGRALEPLVAQRGNDAESALAAAARRALAERTQRESLARLAVL
                     GLPLRKLPKVPGGAGSPAALAALVPHLF"
     gene            3298..>3600
                     /locus_tag="VFQ35_28625"
     CDS             3298..>3600
                     /locus_tag="VFQ35_28625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934717.1"
                     /translation="MRRCSSWSFPIACVVSFVACNSKPSTKPASPAASAALAAASASA
                     RLPPPKRHDWPMPSGPMLAVLAGQGVGPIRIGATTATIERLMELPCDVKTESLCRYV"
BASE COUNT          622 a         1180 c         1183 g          615 t
ORIGIN      
        1 catcgatgct gaccgcgacg acgtcgatgc gattgggacc ccaagaggca gccgagggtt
       61 ttccctgaat ctggagggac gagctgacgt ccttccaggc ttcccagccc gcttcttgcc
      121 atgatttctg gtacagcgtg cgcccggacc tgatgaagac gtccagtcga tcggagtccc
      181 acgccgcgat cgccggagcg ctcacgccat cgcggtttgg gcgacccaga gactcggttg
      241 cagcgctcca cacgccatct tcgtaagagc cgtgatacac gtagccgttc gccttcaccg
      301 cgacgaagtc gaggctgtgt tccttccgag atacggcggc tggatccgag atgaagtcgc
      361 cgccaaggtc cgtccagtca ccgactgacc aagtgtcgtc gtcgctcgca tagaccttgt
      421 aatagagatg ccggtcgtcg ccgcgaacca gagcgatgag cccggaggtg ccccaacgcg
      481 cgatggcagg agacccaacg aggcgaacct ccgaggtcag gcgcgtgtcg agtggctgcg
      541 tggggtccgg atcggctcgt accatcggct gaaacgcgga ccacttgcta tgcgccgcgt
      601 gatagcgctc gatgaacgac gcggcgtcga gcggactgat gtagctgaag ccgcctccgg
      661 cgagcggcgt gggggttggg cccgggacga aagcgttccc gcttcgcttg accaagtcgc
      721 agccagaagt gtccgcttca gtgcacgtac cgccggtcgg cgcccgtgac ggagcctgca
      781 tgatggagtc gaagtcgtaa gggcctatcg tctgctgcgc gagcggcggg ccttccggcg
      841 cgcagtgcga cactatctcg ctgaaatgtc gtcgtgagat cgtcggttcg gggtcgctgc
      901 aaagctgagg ttcgcgaacg gcgaggtacc gatcgcggtc cgcgcgctgc tgctcgttca
      961 gcatgcccag cacgcgtccg agctcgcgtt gcgtcgtcgc ttcgtcgcag ccccgcaagc
     1021 tcacttcttg cactccgttc acgagcgtct gaccgaaagg agcccaacaa cgatcgccgg
     1081 caacgaagcg taggcgtggc ctcacgtcct tgtttggctc gagttcgaag aaagaaatga
     1141 tctgcggagc cgccaacgtc cacgccgtca gggcgtgctg cagctgctcg cgttcgctct
     1201 cgctccagtc cgaaatgctt tcgtagggga ccgggtggtc gaacttccaa agcgcgggag
     1261 tcgtgcaacg catgctgtcg ctgcccattc cgaagtattg agcctcgtct tcgcattcga
     1321 gggccatcat cgcgccattg cccccaccag tagcgactcc gccgcctgtg ccggactcgc
     1381 ctgccatgcc gcctcccact gcagcgactc cgcctctgcc cgcgcttccc ccactcgaag
     1441 cggcgctgct tccgccgtca gaagcgccgc gagttcccgg tgcacctccg gtacgagcgt
     1501 tgtcctgccc accatgccct gcgtcgggct tcgcgctcga aggtgcgccg ccgttcgtcg
     1561 caccaccgct cgcccccgag tcgccgccgg tttgcgagcc accatcgccg atagtcgtcg
     1621 ccccaccgct agcaaagcgt gagcccccgg agttgccaac cgaagttccg ccagcgctga
     1681 gcgcgccggc atcgcccccg cgacctgaag ttgccgttcc attcggttct gggacgtctt
     1741 cgagcggcgc gatgagcgaa cagccgagct cgaaagcaat gactgcgaca gcgctgcgga
     1801 acagagactt ggtgccgctc gcgccgagca cgcgttgacg gaggcttggt ctgagcatga
     1861 tgagcgacgc gagtcgacgc tagcatggct gccaaatgac gcagacccag aaaagggccg
     1921 ctcaccacac ggacttgcgt cctcgcgcgc tgggacgacg ttgggacgac gagagacaat
     1981 tctgggacgg ttcgcacaat tctcggacag ccgtcggaca cagcggcgtt gctcacacga
     2041 cgaactccgt aaaaacggtt cgcggcacac cggttgctaa cgcggcggtg gagttcgaac
     2101 ctgcccaaag ggtagtcggt tagcgggggt gcaggctcga actccgtcga atctgaacca
     2161 tcggtcacat cgcgggccga gctcggagac tgagtatgtt ccaattggtg ctgactccac
     2221 ggggcaccaa tcagttcaga tgcgcctctt gccgcggata gcttgggcaa cacggcgcga
     2281 tgccctcgcg ggttggcgga ggctgctcag aaaaggtgcg gaacgagcgc ggccagcgca
     2341 gcgggagagc ctgcaccgcc tggaaccttg ggtagctttc gcagcggcaa gcccagcact
     2401 gcgaggcgcg cgaggctctc tctctgagtg cgctcggcga gcgcgcggcg cgcggcagcg
     2461 gcgagcgcgg actccgcgtc gttaccacgc tgcgcgacga gcggctcgag cgcgcgccct
     2521 tccgcgtcgg tgaagagctc ttcgaggcaa gaattcgcta cgacctcgac gatgggcagc
     2581 tcgagctctt cgcgaacagc gcgcgcgagc tcgagggtct cgttggtcgg catctcttcc
     2641 ggcaacgtgg cgagcacgac gccgctcgac gccggatcgc gcaagaacgc gatggcgcgc
     2701 tcggcatccg tgcggagccg tcccggtgga gccgcggcca agatcacctt cggcacgcgc
     2761 aacatgtcga ggctgtggcc ggtggcgggc gcgtcgaaga cgacgacgtc gaagcgcaaa
     2821 gcgccgttcg gcagcgtctc gatggcgtgg taccaggctt tgccgagcag cgcccactcc
     2881 ttcaggcccg gcgcgccgtt gaacaagccg gagacgtact tgttgtcgaa gagcgcgttc
     2941 accaccgtct ggctgtgcag gacgagcacg ccgtattcgc gaagggcgac ttcggacgtg
     3001 agcagcacgc cccacaggtt cggggcgaga agctcgatgg aggtgccgag ccgcgggacc
     3061 cctagcagag gcgagagccc ttccttggcg ccgctcgtgc aaagcaagac gcgcttcccg
     3121 cgttcagcga ggccgcgcgc gagggctgcc gagaccgtcg tcttgcccac gccgcctttg
     3181 cccgttacga acagaaaacg gcggcgatcg aagggcaaca tcggggaata aacggggtcg
     3241 cagggtgttc tggcaccgag cagcgtggta catgtcccgc ccggctgcgg aggtcaaatg
     3301 cgtcgctgtt cgagctggtc gttcccgatt gcgtgtgtcg tatcgttcgt tgcgtgcaac
     3361 tcgaaaccca gcacgaaacc tgcttccccc gcggcttcgg ccgccctggc ggccgccagc
     3421 gccagcgcac gtttgcctcc gcccaaacgc cacgactggc cgatgccgtc cggcccaatg
     3481 ctcgcggtgc tggcgggcca gggagtcggt ccgattcgga ttggcgccac gacggcgacc
     3541 atcgagcgct tgatggagct gccctgtgac gtgaagaccg agtcgttgtg ccgttatgtg
//
LOCUS       DASTKS010001335         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1334497, whole genome shotgun sequence.
ACCESSION   DASTKS010001335 DASTKS010000000
VERSION     DASTKS010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334497"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFQ35_28630"
     CDS             <1..463
                     /locus_tag="VFQ35_28630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934718.1"
                     /translation="KAARERLLDWAPRLKGPDARFVALFMREHSVSLAPRAWVDEADE
                     RCTERRARAVFDGMRRADRGALPTLLAYLDAADGHWAERPLAVRAFIDALIRLGAPAK
                     RFTCRNELFDPCRMTSLRGAEQERNARIACLSAARAWLLVNGGLPQSAQGH"
     gene            complement(545..1876)
                     /locus_tag="VFQ35_28635"
     CDS             complement(545..1876)
                     /locus_tag="VFQ35_28635"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HET9934719.1"
                     /translation="MSQNFAEMLVDGHRILPSLLADIEAARASVHISVFLFFRDPIGE
                     EIADALVRKAAQGVTVRVLLNVAKTAMGDPFSTGEKEMMKNDPSVEYDPTDVKPLCKR
                     LAAAGVLVHDTNIDYDADPPGISPRLRSIAARVRSAIAIDDVHIDHRKIIIIDGRIGY
                     CGGANLGAQYLHHDAFDPSKDARVEAAERKARSQREPWWKWHDSLTRFEGPIVQRLEA
                     AFHERWSLDGGEPYESESVAGATRAAESPRGFRLYAAEVHCNEPNPRPNDVRELYLRL
                     IAGARRSIFIENPYPYHPAIIDSLCRVKAARPEVRITLIAPARVHNDNSFSQDAQEHE
                     YERLIAQGVEVHEYQHHFTHLKTAVFDERFSIHGSTNLNYRSLEDDKDFELVVLVDSS
                     ELGRWMLENVRDADLRCSRRITREELREGVAGIRKRMRHPWTLALCSQRML"
     gene            2191..>2404
                     /locus_tag="VFQ35_28640"
     CDS             2191..>2404
                     /locus_tag="VFQ35_28640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934720.1"
                     /translation="MGHLRSCTVALALLMLAPGCAQKPYLHWQGGDAFGVFEGGGKAP
                     SSIDCRGTTEQRFSCFSARVRERAGEA"
BASE COUNT          424 a          768 c          786 g          426 t
ORIGIN      
        1 caaagccgcg cgcgagcggc tccttgattg ggcgccgcga ctcaaagggc ccgacgcgcg
       61 tttcgtcgct ctcttcatgc gcgagcactc ggtttcgctc gctccgcgcg cgtgggtcga
      121 tgaagccgac gagcgatgca ccgagcgcag agcgcgtgcc gtattcgacg gtatgcggcg
      181 ggcagatcga ggagcgctgc cgacattgct cgcatacctg gacgcggcgg acggacactg
      241 ggctgagagg ccgctcgcag ttcgggcctt catcgacgcg ctgatccgtc tcggtgcgcc
      301 cgccaagcgg ttcacgtgcc gaaacgagct tttcgatcct tgtcgcatga catctttacg
      361 gggagcggag caggaacgaa atgctcgaat tgcctgcctt tctgcggccc gcgcctggct
      421 gctcgtcaac ggcgggctcc ctcaaagcgc gcaagggcac tgacaccgca atcggcttac
      481 gtccgctcga gcctttacgc gacctggggc cggtgctcgc ttacgacggc gtcacggccg
      541 cgagtcaaag catgcgctgc gaacagagcg cgagcgtcca cgggtgacgc atgcgctttc
      601 ggatgccggc cacgccctcg cgcagctctt cccgcgtgat gcggcgagag cagcgtaagt
      661 cggcatcgcg aacgttctcc agcatccaac ggccaagctc cgacgagtcg accaaaacga
      721 cgagctcgaa gtccttgtcg tcttcgaggc tccgatagtt gaggttcgtc gagccgtgaa
      781 tcgagaagcg ctcgtcgaag accgccgtct tcaaatgcgt aaaatggtgc tgatattcgt
      841 gcacctcgac cccctgcgcg atgaggcgct cgtactcgtg ttcttgtgcg tcttggctga
      901 acgaattgtc gttatggacg cgcgcgggcg cgatcagcgt gatgcgcacc tcgggacgag
      961 cggccttgac gcgacacaat gaatcgatga tcgcggggtg atacgggtac ggattctcga
     1021 tgaagatgga gcggcgcgcg cccgcgatga gacggaggta gagctcgcgc acatcgttgg
     1081 gccgcgggtt cggttcgttg cagtggacct ccgcggcgta tagccgaaac ccgcgaggcg
     1141 actcagcggc cctcgttgcg cccgcgaccg attcggattc gtagggctct ccgccatcca
     1201 acgaccagcg ctcgtgaaac gccgcttcga gtcgctgaac gatcggccct tcgaagcgag
     1261 tcaggctgtc gtgccacttc caccacggct cgcgctggct gcgcgctttg cgttctgccg
     1321 cttcgacccg cgcgtccttg ctgggatcga atgcgtcgtg gtgcaggtac tgcgcgccca
     1381 ggttcgctcc cccgcaatag ccgatgcgac cgtcgatgat gatgatcttg cggtggtcga
     1441 tgtgtacgtc gtcgatcgca atcgcactgc gcacccgcgc cgcgatcgaa cgcagccgcg
     1501 gcgagatccc gggtggatcg gcgtcgtaat cgatgttcgt gtcgtgcacc agaacacccg
     1561 ccgccgcgag ccgcttgcac agcggcttca cgtcggtggg atcgtactcg acgctcggat
     1621 cgttcttcat catctccttt tcgccggtcg aaaagggatc ccccatcgca gtcttggcaa
     1681 cgttcagtag gacccgcacg gttacgccct gcgcggcctt ccgaaccagc gcatcggcga
     1741 tctcctcgcc gatgggatcg cggaaaaaca ggaacaccga gatgtgtacc gacgcccgtg
     1801 ccgcctcgat gtcggccaag agagatggca aaatgcggtg tccgtccacc agcatctcag
     1861 cgaagttctg gctcatcgct gaacgtgcct gcagcaattt gcgtgcccgc gcgtcctacc
     1921 gcgtgagggc gagaagacgt cacccaattg agcctgcatc gcgcgcgcgg ccggagaaaa
     1981 tcgcgacgca gggccggaag acgggccagg agcggaacga gacgacagcg tcgtcgcctc
     2041 gcgcgaggcg gcttggtcac cgcaagcgca cccgcgtctt gaccgggctc gagagcggct
     2101 caccccggca acgagcaaaa gctatcaggc gatgccgcgc aggagcggaa gtcggagacg
     2161 cgccgcgccc aagtggtatg gacacggggg atgggccatc tccgtagttg caccgttgcg
     2221 ctcgcattgc tgatgctcgc cccgggctgc gcccaaaagc cgtacctgca ctggcagggc
     2281 ggtgatgcat tcggggtctt cgagggcgga ggaaaagccc cgtcgtcaat cgactgccgc
     2341 ggcacgacag agcaacgctt ttcgtgcttc agcgcgcgag tgcgtgagcg ggccggggaa
     2401 gcga
//
LOCUS       DASTKS010001336         6409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_886047, whole genome shotgun sequence.
ACCESSION   DASTKS010001336 DASTKS010000000
VERSION     DASTKS010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886047"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VFQ35_28645"
     CDS             complement(<1..1032)
                     /locus_tag="VFQ35_28645"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9934721.1"
                     /translation="MTHAPVHPYAARKSMRVEPPSDPHSASARTMPTVWGVAWPLLMS
                     LLLTMSLNFVDSFFLAKISDQAAAAAGALFPLLGATVVVFVAVGQAGSSVAAQLIGGR
                     RHEEVPTVYLALVLFNLACGLLTSGTFWLLHRRLPSLLGLHGAIYEQACTYLGIIGSF
                     QFLKSVQIGYANILNSRGKTKWALAEAAITNVCNVALNVGFMTGAAGLPKLGVAGVAL
                     STVLSLALGMVFTMGVVHLHFGVRFPWPPNRALFTNRLRRVLEIGLPSAVEPLAYQSA
                     QTTINALIAGWGAAALAARTYVFNFIMVSTILWSVAFGIATQVVVAHRVGAEDFDGAK
                     REMSRSLRFG"
     gene            1340..3337
                     /locus_tag="VFQ35_28650"
     CDS             1340..3337
                     /locus_tag="VFQ35_28650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9934722.1"
                     /translation="MFERTEWDDAFLRGGGPSAEVVLARTFAELWASNGAGLANEQAS
                     SLFQLSPVALVEVDASGRVELANPSAQRLLEIAATGVLVSELEQDTLVSERILQVARA
                     ARLHGRAVAGLELHGRSYQVEGRAGTGADRALLSFIDAEHVMADGEMSRSARALRAML
                     RAAREGVALLAEQQVLYANAAWYKLTGPSTESSFLSEVVEGKSLATRLSELAAADSDQ
                     TIDVRLGEGTSNEVMAELTSAFVEYQGRPCVLVMARDLTARRRLEARVARSERLASLG
                     MLVAGVAHEINNPLTYALGNLESLAARMESSTEAFSQDDAREAVRDAIDGCRRIATII
                     RELRSFHRADDNEIVLVDPNQVVRDAVRIVGPKCAAIARLEMDLAEVPPVLGVATSLV
                     QVLINLIVNAVDAMSERGPESNLIRISTGSRGTDVRIEVSDNGRGIAAADLSRIFEPF
                     FSRGKTGGTGLGLTISRNLIEQLNGWIDVDSRPGAGTRFIVHLEAARAREATQTSEPP
                     MPPPTLLSELRVLVVDDEPLVARSVARLLAQAQEVVCAHSVAGALDILSARDDIDVVV
                     SDWVMPDGGAQSLMRHLDYRGQGRVPLVVMTGLGQLGGREPVEGPCVRKPVSRTALLN
                     GIAHALGLFGRNSMVVRTDTVRRAEYSLIAPPDSVSQRGRR"
     gene            3546..4055
                     /locus_tag="VFQ35_28655"
     CDS             3546..4055
                     /locus_tag="VFQ35_28655"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9934723.1"
                     /translation="MLLSPRVAASVLCVVVSMACGASQPPPPTHPSPLLGYPMPSFES
                     RTISGNPVISASFERHKVVVTFVASKCKACEQTLSAANAVYKDDRELVVVGVYPEKRA
                     EGAVRVASRLELRFPVVVDEDGVIARRFQVGDAVPTTFVVDSEGRVQWVGGADLTAEA
                     LERAVAAAR"
     gene            complement(4182..5180)
                     /locus_tag="VFQ35_28660"
     CDS             complement(4182..5180)
                     /locus_tag="VFQ35_28660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934724.1"
                     /translation="MRTERGLERSLNQGTHFELLYELSSSSLGALWLGRTVGGRESGR
                     PVLLRALEPGALNRLEGALMRAATIAHPRLLKTLGVTRIDAVTYLVSEYVEGLPLSEL
                     ARPMHAAAASVVPEVALRITLDALLAVAAARDELARFGLKSLPRCLFADTAWVASFGE
                     TLLSDAGIACELLNQRPAASADQPSDVFAAGSLLWELLSGKRLSNATDLERVPSIEGL
                     RALAPSPLPLLQVVARAVSLDPDKRFASVEAMAEAIQDLPVHWIATDAEVRAAVEVLA
                     HSMPSTMGADGASSGEHEIDPWDTPTRSLRMRPLPTANAQEAATVPPPVPERGPRS"
     gene            complement(5319..>6409)
                     /locus_tag="VFQ35_28665"
     CDS             complement(5319..>6409)
                     /locus_tag="VFQ35_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET9934725.1"
                     /translation="LTRNGFDGAYSMLYHARRPHEALPIAPESAPKTFELPTDAGPAQ
                     LLRRHYRSLSLEVGGGPALGARRPLLFNSDVVIGVARPDAADDAYFSNGDGDELFFVQ
                     RGSGFVRSQFGDLRFSSGDYVCIPRGVLHRWVPESSVEQAYLYLECRGGLFIPPRFRN
                     GTGQLRMDAPYSHRDFGRPVFVGPLDEGHRLSCVKRGDRFHFYRFEHSPLDVLGWDGS
                     VYPFSFQIRKFQPRVGLVHLPPPVHTTFEARGVAICSFVPRPLDFHPLANPCPYPHAS
                     VDVDEVLFYANSAFGSRRGVGEGSISHHPAGILHGPHPGAYENVPTTHATEELAVMLD
                     CMEPLRATPTARECEDAEYHGSFRAGQR"
BASE COUNT         1105 a         2053 c         2192 g         1059 t
ORIGIN      
        1 gccgaaccgg aggctgcggc tcatctcgcg tttggctccg tcgaagtctt ctgcgccgac
       61 ccgatgcgct accacgacct gcgtcgcgat gccgaacgcg acgctccaga ggatcgtgct
      121 caccatgatg aagttgaaca cgtacgtgcg cgctgcgagc gcggccgcgc cccagccggc
      181 gatgagcgcg ttgatggtcg tctgcgcgct ttgataggcg agcggctcga ccgcggaggg
      241 cagcccgatt tcgagcaccc gccgcaggcg attggtgaac aacgcgcggt tcggaggcca
      301 ggggaagcgc acgccgaagt gcagatgcac gacgcccatc gtgaacacca tcccgagagc
      361 gagcgagagc acggtcgaaa gcgcgacgcc agcgacgccg agcttcggta gacccgctgc
      421 gccggtcatg aacccgacgt tcaacgcgac gttgcagacg ttggtgatcg cggcctcggc
      481 gagcgcccac ttggtcttgc ctcgtgagtt caggatgttg gcgtacccta tctgcactga
      541 cttcaggaac tggaagctcc cgatgatgcc gagatatgtg cacgcctgct cgtagatggc
      601 gccgtgcaag ccgagcaggc tcgggagtcg tcggtgcagc agccaaaaag tcccgctcgt
      661 cagtagaccg cacgccaagt tgaagaggac gagcgcgagg tacaccgtcg gcacctcttc
      721 gtggcgccgc cccccgatga gctgcgcggc cacgctcgag cccgcttgtc ccaccgcgac
      781 gaacacgacc acggttgctc cgagcagcgg gaacaacgcg cccgccgcag ccgctgcctg
      841 atcggagatc ttcgccagga agaacgagtc gacgaagttc agcgacatcg tcaagagcag
      901 gctcatcaag agcggccacg ccactcccca cacggtcggc atggtccgcg cggaggcgct
      961 gtgagggtcg ctcgggggtt cgacgcgcat cgactttcgc gccgcatagg ggtgcactgg
     1021 ggcgtgcgtc aatcgaatag ccaggcacgc agcgtttctg cggcgctgca gcgtgcgacg
     1081 gcgaggctcg ccgaggcaac acgatggtgg tggagtgttt cgagttggtt ctcacggtaa
     1141 ttgcgaccgc cgctacttgc ggttcgttcg acacggagaa ctgggctgcg aaaacgcggt
     1201 gaggtcgagg tgtacacgtc tcccacatga catcggcgcg aacgccgaaa cgcgcgcgct
     1261 catactcgcg agttggtccg gcgctgtcac gtcgatgcgc gttgacggag ctcgagaacg
     1321 cagcgacact cggcgcaacg tgttcgaacg aaccgagtgg gatgacgcgt tcctacgcgg
     1381 tggcgggccc agtgcggagg tcgtgctcgc gcggaccttc gccgaattgt gggcttcgaa
     1441 cggcgccggc ctcgcgaacg agcaggcgtc gagcttgttc cagctctcac cggtggctct
     1501 ggtagaggtc gatgcgagcg gaagagtaga gctcgccaac ccgagcgcgc agaggctact
     1561 cgaaatcgct gcgaccggtg tcctggtcag cgaactcgag caagacacgc tagtcagtga
     1621 gcgaattctg caagttgcgc gagcggcgcg gctccacggg cgggcggtgg cgggtctgga
     1681 gctccacggg cgatcgtacc aggtcgaggg ccgcgccggt acgggagcgg accgcgcgct
     1741 gctctcgttc atcgacgcgg agcacgtcat ggccgacggt gaaatgtcgc gaagcgcgcg
     1801 agcgttgcgc gccatgctgc gcgccgcgcg cgagggagtg gcgctgctcg ccgagcagca
     1861 ggtgctctac gcgaacgcag cctggtacaa gctgacgggc ccctccaccg agagctcatt
     1921 tctgtccgaa gtggtcgagg gaaagtcgct ggcgacccgc ttgtcggagc tcgccgccgc
     1981 cgattcggat caaaccatcg acgtccgcct cggcgaaggc acgtcgaacg aagtgatggc
     2041 cgagctcact tcggctttcg tggagtacca ggggcgaccc tgcgtgctgg tcatggcgcg
     2101 cgatctcacc gcgcgacgtc gcctcgaggc tcgggttgct cgcagcgagc ggctcgcctc
     2161 gctcggcatg ctcgtcgcgg gcgttgcgca cgagatcaac aatccattga cctacgcgct
     2221 cggaaacctc gagagcctcg cggctaggat ggagtcttcg acggaagcgt tctctcaaga
     2281 cgacgcgcgc gaggccgttc gcgatgcgat cgacggctgt cgccgcatcg cgacgatcat
     2341 cagagagctc aggtcgtttc accgcgccga cgacaacgaa atcgtgctcg tcgacccgaa
     2401 ccaggtcgtc cgagacgccg tgcgcatcgt cggaccgaag tgcgccgcca tcgcgcgtct
     2461 ggaaatggac ctcgcggaag tgccgcccgt actcggggtt gcgacgagtc tcgtccaggt
     2521 gctcatcaac ctcatcgtga acgcggtcga cgcaatgtcc gagcgcgggc ccgagtcgaa
     2581 tctaatccgc atctcgaccg gatcgcgcgg cacggacgtg cgcatcgagg tctcggacaa
     2641 cggccgcggc atcgcagcgg cagatctttc acgcatcttc gagcccttct tctcgcgcgg
     2701 gaagacgggc ggcacggggc tcgggctcac gatcagccgc aacctcatcg agcagctgaa
     2761 cgggtggatc gacgtggatt cgcggcccgg cgccggtacg cgtttcatcg tgcacctcga
     2821 agccgcccgt gcgcgcgagg cgacgcaaac gagcgagccc ccgatgcccc cgccgacctt
     2881 gctttcggag cttcgagtcc tcgtcgtcga cgacgagccg ctggtcgcgc gctcggtcgc
     2941 acgcctgctg gcgcaagcgc aagaggtggt gtgcgctcac tcggttgcgg gcgcgctcga
     3001 cattttgagc gcgcgagacg acatcgacgt cgtcgtttcg gattgggtga tgcccgacgg
     3061 cggcgcccag tcgctgatgc gtcacttgga ttatcgcggc caagggcgag tcccgctggt
     3121 cgtcatgact ggactggggc agcttggggg acgcgaaccc gtggagggcc cctgcgttcg
     3181 caagccggta tcgcgcacgg ccctgttgaa cggcatcgcg cacgcgctcg ggctgttcgg
     3241 gcgaaatagt atggtcgtgc gcaccgatac ggtgcgcaga gccgaatatt cgttgattgc
     3301 tccacccgac tcggtgagtc agcgcgggcg gaggtagccc aaagcttcgg cgcggggctt
     3361 ccgcgcgaaa ttgcctcgtg ccgagcttct gtcgcgaaaa gcagcgtcct ttgtggggta
     3421 atctcccgca tgggaaatcg gctcaaataa atcggttcga gcgggttcga agggacgctt
     3481 ccgattcgcg ctcggttcgc cacctttcaa tttgcgaacg actccgacta tgttttcgcg
     3541 agcctatgct gctgagcccg cgagtcgctg catccgttct ctgtgtcgtg gtttcgatgg
     3601 cgtgtggggc ctcgcaaccg ccgcccccga ctcatccaag cccactgctc ggctacccga
     3661 tgccgtcgtt cgagtcgcgc accatcagcg ggaacccggt gatcagcgct tccttcgagc
     3721 ggcacaaggt cgtcgtgact ttcgtggcgt cgaagtgcaa ggcgtgcgag cagacgctca
     3781 gcgcagccaa cgccgtttac aaagacgacc gcgagctcgt ggtcgtcggg gtctacccgg
     3841 aaaagcgcgc agaaggcgcg gtgcgagtcg cctcgcggct cgagctccgc tttccggtcg
     3901 tcgtcgacga ggacggggtc atcgcgcgtc gcttccaagt cggtgacgcg gtgccgacca
     3961 ctttcgtcgt cgacagcgag ggtcgcgtac agtgggtcgg cggggcggat ctcaccgccg
     4021 aggcgctcga gcgagcggtg gccgcggcgc gttgagcagt ggtcgaggga ttgcgtcgcc
     4081 cgccggcgcg ctcgagcgag cggtgaccgc ggcgcgttga gcagcggtcg agcccttgag
     4141 tcgcgcgcgc gggaagtctc cccccgcact tttggctctg ctcagctgcg cggcccgcgt
     4201 tcgggaaccg gaggaggcac ggttgcggcc tcttgagcgt tcgcggtcgg caacggacgc
     4261 atgcgcaggg aacgcgtcgg cgtgtcccat gggtcaatct cgtgctctcc ggaagaggcg
     4321 ccgtccgcgc ccatcgtgct cggcatcgag tgcgcgagca cttcgacggc ggctcgtacc
     4381 tcggcgtcgg tcgcgatcca atgcaccgga agatcttgaa tcgcttcggc catggcctcg
     4441 acgctggcga agcgcttgtc cggatccaag ctcaccgcgc gcgccacgac ttgcagtaac
     4501 gggagcgggc tcggcgccaa cgccctgagt ccctcgatgc tcggcacccg ctccaggtcc
     4561 gtcgcgttcg acaggcgttt cccgctcaag agctcccaca agagcgaacc cgcggcgaat
     4621 acgtcgctcg gctgatctgc gctcgcagcg gggcgctggt tcaagagctc gcaggcgata
     4681 ccggcgtcac tcagcagtgt ctcgccgaag ctcgcgaccc aggccgtgtc tgcgaacaag
     4741 cagcgaggta acgacttcag gccgaagcga gcgagctcgt cgcgcgcggc cgcgaccgcg
     4801 aggagcgcgt ccagcgtgat gcgcagcgcc acttcgggca cgaccgacgc cgccgccgcg
     4861 tgcatcggtc gagcgagctc cgagagaggc agaccttcga cgtactccga tacgagatac
     4921 gtgaccgcgt cgatgcgtgt caccccgagc gtcttcagca gccgagggtg agcgatggtc
     4981 gccgcgcgca tgagggctcc ctcgagccga ttgagagcgc ccggctcgag cgcgcgcagc
     5041 agcacgggcc tgccggattc gcgcccgccg acggtgcgtc cgagccagag cgcacccaag
     5101 ctgctgctcg agagttcata gagcaactcg aagtgagttc cctgattcag agaacgctcg
     5161 aggccacgct cggttcgcaa gttcgtcttc gctccttacc cttcaacggc atgcgcttca
     5221 ttcacttaag cgctgtgggc ggaatccgcg acgtgactgg gcagctgttc agagcgtcgt
     5281 attccgcgcc gcgcgcgcgc acgtcgagcg cgcgacgctc accgttggcc agcgcggaaa
     5341 ctgccatggt actcggcgtc ttcgcactcg cgcgcggtcg gagtcgcgcg aagcggctcc
     5401 atgcagtcga gcatgaccgc gagctcttcc gtcgcatgag tcgtggggac gttctcgtac
     5461 gcgccggggt gcgggccgtg caggatgcca gccgggtgat gcgagatgct gccctcgccg
     5521 actccgcgac gcgagccgaa tgccgaattc gcgtaaaaga gcacctcgtc gacgtcgacc
     5581 gaagcgtgcg gatatgggca ggggttcgcg agtggatgga agtccaaggg ccgcgggacg
     5641 aagctacaaa tcgcgacccc gcgagcttcg aacgtcgtgt gcacgggtgg cggcaggtga
     5701 acgagcccga cgcgcggttg gaacttgcga atctgaaacg agaatggata aacggagccg
     5761 tcccagccga gcacgtccag gggcgaatgt tcgaatcgat agaagtggaa gcgatcgccg
     5821 cgcttcacgc acgagagccg gtggccttcg tcgagtggtc cgacgaacac ggggcgcccg
     5881 aagtcgcggt gcgagtaggg cgcgtccatc ctgagctgac ccgtgccatt tcgaaagcgc
     5941 ggcggaatga acaaccctcc gcggcactcg aggtagagat acgcttgttc gacggacgat
     6001 tcgggcaccc agcggtgcag tacgccgcgc ggaatgcata cgtagtcgcc gctcgaaaag
     6061 cgcagatctc cgaactgtga gcgaacgaac ccgctcccgc gctgcacgaa gaagagctcg
     6121 tcgccgtctc cgttgctgaa gtaggcgtcg tcggcggcgt cgggtcgcgc gacaccgatc
     6181 acgacgtccg agttgaacaa gagtggccgc cgcgcgccga gcgcaggccc tccgccaacc
     6241 tcgagtgaaa gcgacctata gtgtcggcgc aagagctgtg cggggccggc gtcagtcggg
     6301 agctcgaagg tcttcggcgc cgattcgggc gcaatcggca gcgcttcgtg cgggcgccgc
     6361 gcgtgataca gcatcgaata ggcgccgtcg aagccgttgc gggtcaggc
//
LOCUS       DASTKS010001337         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_663941, whole genome shotgun sequence.
ACCESSION   DASTKS010001337 DASTKS010000000
VERSION     DASTKS010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663941"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1264)
                     /locus_tag="VFQ35_28670"
     CDS             complement(59..1264)
                     /locus_tag="VFQ35_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET9934726.1"
                     /translation="MPSRSRSYAVIALVASLLGLLFASNSTLDYASHLDRRLHDVHCS
                     FIPGASVTSEAEACRAAMYSPYSALLKEAYWGGIPISLFAMGAFAFFVGFSLYLVMSG
                     PRAPRAAVLFFSGVSATPFLVSLVMFIISLTKLGHLCKTCVGIYISSTLLLVAGIMGA
                     ASVLRELDETPRPRVGFMLPVAWLLALGLTTLVPAVVYAASVPDERPYLTQCGTLKVE
                     PAANVELVPLKGARAVQPALFFEDPLCPTCKAFHERLVSEGIYDRLDARLSLFPLDSE
                     CNWMLNEPLHPGACIVAKAVICGGEQSRAVLEWAYDKQEDVQKAGKQGPKALKSLIRA
                     RFGDPITRCIDERKTDTRLNNHLHFASENNIPVSTPQVYLGKQRLCDEDTDLGLRFTL
                     GQLAPEVLK"
     gene            complement(1325..2617)
                     /locus_tag="VFQ35_28675"
     CDS             complement(1325..2617)
                     /locus_tag="VFQ35_28675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934727.1"
                     /translation="MGDTPAPASKRKDSSASTSAPLPESQTKLNQAMSTAPPTDDIDS
                     EWPSDDEPAFEPKVEALQKVASATPEPSRPTSDGPPWQETDEGAKALTAPRFPSDPGL
                     PRDLRSPSSVPSSRPALAVEAPPPSKRDMDTVPAAPQASRPPNSSGVSSSRVRASADA
                     VDAAEAMAVSARLRASQSITEAAPPSAPAPTAQARFESSPAPVVTLPSQGPESRRDPS
                     ADARHSFDPHVHLSQPVEARKPSRAPLLFALVASAAAAVLLMQAGGGSTSSTTSSAAS
                     SAVSNSAPPVAEAPSAHVANAEPKPEGKAEPAKSEAKEELPKPEPAKPEQPSAAPASA
                     AVVEAPKAEAETTATASPASTGNISVLIRVKPPQARIFYRGKEVGKPPVTVEIEPGKR
                     RAYEVSLPGFMTRKVVVDGSETELSIGLRPDPSAPAPK"
     gene            complement(2737..>3289)
                     /locus_tag="VFQ35_28680"
     CDS             complement(2737..>3289)
                     /locus_tag="VFQ35_28680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9934728.1"
                     /translation="ESAVAERTAGLIALKKGDDALAFKHFAKASDLDPNDTTARLNIG
                     TVLLQAGVYDRAAEQFRAVHEAEPDDVAATLGLAAARRGLAKKDDAQALGEVEKLLKS
                     VLEKEPDNLAATFNLAILYADYAKRPADAAPLFRRFLEQAPASHPARPEVEKWLSAQN
                     SAPSPAAAPPDTAAPAAKPASKK"
BASE COUNT          555 a         1037 c         1130 g          567 t
ORIGIN      
        1 gtcccgacga tgccagctag gtgggccgcc gaggacgttg ctccgcccgt gtcgtcagtc
       61 atttcaagac ctccggagcg agctgaccca aggtaaaccg caggccgaga tccgtgtctt
      121 cgtcgcacag ccgctgcttg ccgaggtaaa cctgaggagt ggagacggga atgttgttct
      181 cggaggcaaa gtgcaggtgg ttgttcaggc gggtgtccgt cttccgctcg tcgatgcagc
      241 gcgtgatggg gtcgccgaag cgcgcgcgga tcaacgattt gagcgccttc gggccttgtt
      301 ttccggcttt ttgcacgtcc tcttgcttgt cgtaggccca ttcgaggacg gcgcgcgact
      361 gctcaccacc gcagatcacg gctttggcga cgatgcaggc gccggggtgc agcggctcgt
      421 tcaacatcca gttgcactcg ctgtcgagcg ggaacaacga gaggcgcgcg tcgaggcgat
      481 cgtaaatccc ctcgctcacc aagcgctcgt gaaaggcctt gcacgtcggg cacagcgggt
      541 cctcgaagaa cagcgcgggc tggaccgcgc gcgcgccctt cagcggcacg agctcgacgt
      601 tggcagcggg ctcgaccttc aacgttccgc actgagtcag gtacgggcgc tcgtcgggca
      661 cgctcgccgc gtacacgacc gcggggacca gcgtcgtcag gccgagcgcc aagagccaag
      721 ccaccggcag catgaaccca acgcgaggcc gcggcgtctc gtcgagttcc ctcaacaccg
      781 acgcggctcc catgatgccc gcgacgagca gcagcgtgga cgagatgtag atgccgacgc
      841 aggtcttgca caaatgcccg agcttcgtga ggctgatgat gaacatcacg agcgagacca
      901 ggaacggcgt cgcgctcacc ccggagaaga acagcaccgc agctcgcggg gcacgtggac
      961 cggacatgac gagatagagg ctgaagccaa cgaagaaggc gaaggccccc atcgcgaaga
     1021 gcgatatcgg gattcctccc cagtacgctt ccttcaacaa agccgagtac gggctgtaca
     1081 tcgccgcgcg gcacgcctcc gcttcgctcg tcaccgaggc gcccgggatg aagctgcaat
     1141 gcacgtcgtg caggcgccgg tcgaggtgcg acgcatagtc gagcgtcgaa ttggacgcga
     1201 aaaggagccc cagcaaggag gcgaccaaag caattacagc gtaactgcgc gaacgcgagg
     1261 gcatcggccc ggagcgttgc aacagctcct ctgctggtca agttccgggc agacggcggc
     1321 gggtttactt gggggcaggt gcagaaggat ctgggcgcaa tccgatggat agctcggtct
     1381 cgctgccgtc gaccacgact ttgcgagtca tgaagccagg cagagagacc tcgtaggcgc
     1441 ggcgcttgcc aggttcgatt tcgaccgtca ccggaggttt gccgacctct ttccctcggt
     1501 agaagatgcg cgcttggggc ggcttcactc ggatgagcac gctgatgttg ccggtgctgg
     1561 ctgggctcgc cgtagccgta gtttcggctt cggctttggg tgcttcaacg acggcagcag
     1621 aagccggcgc cgcgctgggc tgctccggct tcgcgggctc cggcttcggc agctcttctt
     1681 tcgcctccga cttcgcgggc tctgccttcc cctccggctt cggctctgcg ttcgcgacgt
     1741 gcgcgctcgg agcctcggca acgggcggcg cggagttcga aacagccgaa gacgcagccg
     1801 aagacgtcgt cgaagacgtc gacccgcctc cggcttgcat cagcaggacc gcggcggctg
     1861 ccgaagcaac cagcgcgaaa aggagcggcg cgcgcgacgg cttcctcgct tcgacgggct
     1921 gcgaaaggtg gacgtgcggg tcgaagctgt ggcgagcatc ggcgctcgga tcgcgtcgcg
     1981 actcggggcc ctgactggga agagtcacca ccggagcggg gctcgactcg aatcgcgcct
     2041 gcgcggtcgg cgcgggagcg ctaggcggcg cggcctccgt gatcgactgg gatgctcgca
     2101 gccgtgcgct gacggccatc gcctcagccg cgtcgacggc gtccgcgctg gcccgaacgc
     2161 ggctcgagct cacgcccgag ctgttcgggg ggcgagaagc ctgcggcgca gccggcaccg
     2221 tgtccatgtc gcgtttcgat ggaggcggag cttcgactgc gagcgccgga cgagacgacg
     2281 gaacgctcga cggcgagcgc aggtcacgcg gcaaacccgg atctgacggg aagcgcggcg
     2341 cggtgagagc cttggcccct tcgtctgttt cctgccaagg cgggccatcg ctcgtcggtc
     2401 gagatggctc cggggtcgcg gacgctactt tctgcagagc ctcgactttt ggctcgaacg
     2461 ccggctcgtc gtcgctcggc cactccgaat cgatatcgtc ggtcggcggt gcggtcgaca
     2521 tcgcctggtt gagcttggtc tgggactcgg gcaagggcgc gctcgtgctc gcggaggagt
     2581 ctttgcgctt ggaggcggga gctggtgtgt cgcccatggc aggctctttc agcatacgtg
     2641 cgatctcagg ggaatggcga gggcagcgaa cgccctggat tggactacat aaatttgcga
     2701 ccgagctgca tcaccctaga tggtgatttc cgcgacctat ttcttcgacg cgggctttgc
     2761 ggcgggagcc gccgtgtcgg gcggagcagc ggccggagat ggagcagaat tctgcgcaga
     2821 aagccacttt tcgacctcgg gacgcgcggg gtggctcgcg ggcgcttgct ccaaaaagcg
     2881 acgaaagagc ggagccgcat ccgcgggccg cttcgcgtag tccgcgtaca aaatggcgag
     2941 attgaacgta gcggcaagat tgtcgggctc tttctcgaga accgacttca agagtttctc
     3001 gacctcaccg agcgcctgag cgtcgtcttt cttcgctagc ccacggcgcg ccgccgcgag
     3061 acctagtgtc gccgcgacgt cgtcgggttc cgcctcgtgc acggccctga attgctccgc
     3121 cgcgcgatcg tacacgccgg cctggagcag cacggtaccg atgttgaggc gcgccgtcgt
     3181 gtcgttcgga tcgagatcgc tcgccttcgc gaagtgcttg aaagcgagcg cgtcgtcgcc
     3241 cttcttcaaa gcgatgagcc ccgccgtgcg ctcggcgacg gcgctctcc
//
LOCUS       DASTKS010001338         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1559764, whole genome shotgun sequence.
ACCESSION   DASTKS010001338 DASTKS010000000
VERSION     DASTKS010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559764"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFQ35_28685"
     CDS             complement(<1..647)
                     /locus_tag="VFQ35_28685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9934729.1"
                     /translation="MLGKLEPPRTAPNEALPPTKHHLVLVPGFFGFSAFGELAYFNGV
                     REVLVEAFHRRGAEATVSYVTTLPTASIRVRAAKVLDTLARVAAETSGPIHVIGHSTG
                     GLDARLAIAPTASLPTDAGKSFDYERVKTLVSVCAPHFGTPLATFFSSTLGKPLLKTF
                     ARYWAWALERGRIPVHVLLRLGYFVVRLRDPFRKRRSTFDELYEKLLNDLRDERRL"
     gene            814..1920
                     /locus_tag="VFQ35_28690"
     CDS             814..1920
                     /locus_tag="VFQ35_28690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934730.1"
                     /translation="MAIVSALLSALARRLGSLVQALFGWSVTALFGRLPRARQAAVSA
                     TLLLSAAWPLLLIGTVFPAVAAWALAFVPLHKWMNDDVLRIVWAALALAVPLVVGSLT
                     RYAAESRSLRGGKLRSVLAGYALTLGYFLALLITLVTVPLAKLASLARRWDDDHIYVR
                     PREGDYRDAVRSVAEAFALCGLVPEKQAIPPRMSLASRVLKWFAKSSLHPILPEEPQR
                     LVAVGIEAYLYPADLMIRGEQRRLSQLRAMLSRTELERYAYLVESAVAQRMQDELARL
                     WGVWRMHAFPDQAAPRLSQRLLEIYRELNAAEVPYQDWVVLERLLRKFERALAGGPGV
                     LDAEQDGLERAKRAALEIETTLREREQAMTHEPA"
     gene            2006..3028
                     /gene="moaA"
                     /locus_tag="VFQ35_28695"
     CDS             2006..3028
                     /gene="moaA"
                     /locus_tag="VFQ35_28695"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET9934731.1"
                     /translation="MQANPPNQLSDARGRPLGDLRISVTDRCNFRCGYCMPREHFGKG
                     HVFMPRAELLSFEEIARVARVVSKLGVRKLRLTGGEPLLRSELPSLVAMLAEIPEVDL
                     ALTTNGSLLARHAEALARAGLKRVTVSLDAITPAAFQLMADAPYSVDDVLAGIEAAAR
                     AGLGPVKINAVIRRGMNDAEIPLLARKFRGTGHIVRFIEYMDVGITNGWKLDQVVTAR
                     EIVERIHREFELEPVERNYEGEVASRYRYRDGAGEVGVIASVTQPFCGACQRLRLSSE
                     GKLYTCLFGRTGYDLRARLRSGASDEELDGIVRSLWQERNDRYSEQRSEKTRSLPRIE
                     MSYIGG"
     gene            3492..>3898
                     /locus_tag="VFQ35_28700"
     CDS             3492..>3898
                     /locus_tag="VFQ35_28700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934732.1"
                     /translation="MPACGSDEGGSGQTGGAGTAGSRGGNSNNGSGGRSTNASGGRAP
                     SGGAPNQCPSLPKPVEGASCTVDGLACEMSPGALCLCERAATPGNGGATGAGGRRNQQ
                     GRGGAQNTGALTWNCFGRTPPGSGGTGAGGALS"
BASE COUNT          630 a         1244 c         1337 g          687 t
ORIGIN      
        1 agccgccgct cgtcgcgcag gtcgttcagg agcttctcgt acagttcgtc gaaagtgctg
       61 cgccgttttc gaaacgggtc acgtagtcgc acgacgaagt agccgagccg cagcagcacg
      121 tgaacgggga tgcggccgcg ctcgagcgcc cacgcccaat atcgagcgaa ggtcttcaag
      181 agcggcttgc cgagcgtgct gctgaagaac gtcgcgagcg gtgtgccgaa gtgaggcgcg
      241 caaacgctga cgagcgtttt gactcgttcg tagtcgaagc tcttgccggc gtcggtgggt
      301 aggctcgcgg tgggagcgat ggcgagtcgc gcatcgaggc cgcccgtcga atgtccgatg
      361 acgtgaatcg ggccgctggt ttcggcggcg actcgcgcca acgtgtcgag gactttggcc
      421 gcgcgcactc gaatcgaagc ggttggtagc gtcgttacgt agctgaccgt cgcctccgcc
      481 ccgcgccggt gaaacgcctc gacgagcact tcgcgcacac cgttgaagta cgcgagctca
      541 ccgaaggcac tgaacccgaa gaagcccgga accagaacga gatgatgttt cgtgggcggc
      601 agagcttcgt tcggtgcagt acgcggcggt tccaacttgc ctagcatatc gctcttcggg
      661 gtcggagcgc ggcttcaaga gcgcctcagc gcggcgctcc tgttgcgagc tgccgctcga
      721 tccgagcgcg cgtcggcggt cgctctcggc cgaagacttg cggttcggcg aggcgctcga
      781 aaacctggca tcgtccgtgc actgcgacga agcgtggcca tcgtatccgc tctgctcagc
      841 gcgctcgctc gtcgcttggg gagtttggtc caagccctgt tcggttggtc cgtcacggcg
      901 ctgttcggtc gcttgccgcg cgcgcgtcaa gccgcggtct cggccacttt gctcctgtcc
      961 gcggcgtggc cactgcttct gatcggcacg gtgttccctg cagttgcggc gtgggcgctc
     1021 gctttcgtcc cgctgcacaa atggatgaac gacgacgtgc ttcgcatcgt gtgggccgcg
     1081 ctcgcgctcg cggtgccgct cgtcgtgggt tcgctgacgc gctacgcggc cgagtcgcgc
     1141 tcgttgcggg gcggcaagct tcgctccgtc ctcgctggct acgcgttgac gctgggctac
     1201 ttcctcgcgc tcctcatcac tctcgttacg gttccgctcg cgaagctcgc gtcgctcgcc
     1261 aggcgctggg acgacgacca catctacgtg cgacctaggg aaggggatta tcgagacgcg
     1321 gtgcggtccg tcgcggaagc cttcgcgctc tgcgggctgg ttccggagaa gcaagcgatc
     1381 cccccgcgga tgagcctcgc ctcgcgggtg ctcaagtggt tcgccaagag ctcgctgcac
     1441 ccgatcctcc ccgaggagcc gcagcgactc gtggcggttg gaatcgaagc ttacctttac
     1501 cccgccgatc tgatgatccg cggcgagcag cgccgtctca gtcagttgcg agcaatgctg
     1561 agccgcaccg agctcgagcg ctacgcctac ctggtcgagt cggccgtcgc gcaacgcatg
     1621 caagacgagc tcgcgcgtct ctggggtgtc tggcggatgc acgccttccc cgatcaagcc
     1681 gcgccgcggc tctcgcagcg gctgctcgag atttaccgcg agctgaacgc ggccgaggtg
     1741 ccctaccaag attgggtcgt cctcgagcgg ctgctgcgca agttcgaacg agctctcgcc
     1801 ggcgggccgg gcgtgctcga cgcggagcaa gatgggctcg aacgcgcgaa gcgcgcagct
     1861 ctcgaaatcg aaaccacgct gcgcgagcgt gagcaggcca tgacacacga acctgcttga
     1921 gctcaccgcg ggacgcgcgg tagtcccttt cgagcggcga gtttcaccgg gatggatcga
     1981 gcgtgacttg tgctaagtcg taggggtgca ggcgaacccc ccgaaccagc tgagcgacgc
     2041 gcgcgggcga ccgctcggcg atctgcggat cagcgtgacg gaccgctgca acttccgttg
     2101 cggttattgc atgccgcgcg agcacttcgg caagggtcac gtcttcatgc ctcgggcgga
     2161 gctgctctcg ttcgaggaaa tcgcccgtgt agcgcgcgtg gtatcgaagc tcggcgtacg
     2221 aaagctgagg ctcacgggtg gcgagccgct gttgcgttcc gagttgccga gcctcgtcgc
     2281 gatgctcgcg gagatcccgg aggtcgacct cgccctcacg acgaacggct cgttgctcgc
     2341 gcgtcacgcg gaggcgctgg ctcgcgccgg gctcaagcgc gtgacggtga gcctcgacgc
     2401 catcaccccc gctgcctttc agctcatggc cgacgcgccg tactcggtcg acgacgtcct
     2461 cgccggaatc gaagccgcgg cgcgcgcggg ccttggcccg gtgaagatca acgcggtgat
     2521 ccgccgcggc atgaacgacg cggagatccc gctgctcgcc cgcaagttcc gcggcaccgg
     2581 gcacatcgtc cgcttcatcg agtacatgga cgtgggcatc accaacggtt ggaagctcga
     2641 tcaggtggtg acggcgcgcg aaatcgtcga gcgcattcat cgcgagttcg agctcgagcc
     2701 ggtcgagcga aactacgaag gcgaggtcgc gagccgctat cgctatcggg atggcgcagg
     2761 tgaagtcggc gtgattgcga gcgtgacgca gccgttctgc ggagcttgtc agcggctccg
     2821 gttgtcctcg gagggcaagc tttacacctg cttgttcggg cgcaccgggt acgatctgcg
     2881 cgcgcggctc cgaagcggag cgagcgatga agagctcgac ggcatcgttc gcagcctctg
     2941 gcaagagcga aacgatcgct attcggagca gcgctccgaa aagacacgca gcttgccccg
     3001 tatcgagatg agctacatcg gcggctgagg gcgtccgctt gccgcggcgc ggtgggtaag
     3061 cacggggcgc agcgcacgac gacgatctga gtgagtgtaa gagaagtccg acctcgagat
     3121 gccgactgcg cgagcggccg acgattcgtg gaatgtttca gtgccctgcg agtcgccgcg
     3181 cgcggcgcac cgatttcgcc gttgtctcga gccccgaggg ctcacccacg tcgctttcgc
     3241 cctactctcg ctcgaccccg atttcacgcc gagggttcga cgagctgatg cacgagacgt
     3301 tcgtcactgc gctatggaga gaaggagccg acttcgacca gagcgcccgc acgtgttgac
     3361 tggcagggcg ttggtggggc gactagtatt caacccggct tccagtctat cctggaaaag
     3421 gagcgcgcac cttgacccgc caacagacga ggtttgtcct tcttgggctg attctttgca
     3481 acttcggttt gatgcccgct tgcggcagcg acgaaggcgg ctcgggacaa acgggcggag
     3541 caggcaccgc cggttcacgc ggcggcaatt cgaacaacgg aagcggcggt cgaagcacca
     3601 atgccagcgg cggtcgagcg ccgtccggcg gtgcgccgaa tcaatgcccc tccctcccga
     3661 agccggtcga gggagcgtcg tgcacggtcg atggcctcgc gtgcgagatg tcgccgggtg
     3721 cactctgtct ctgcgaacgt gccgccacac cgggcaacgg cggagcgacg ggcgcaggag
     3781 ggcggcgcaa ccagcaaggc cgtggtggtg cgcagaacac cggcgcgctc acgtggaact
     3841 gctttggtcg cacgccgccg ggcagcggtg ggaccggcgc tggtggagca ttgagcat
//
LOCUS       DASTKS010001339         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_440627, whole genome shotgun sequence.
ACCESSION   DASTKS010001339 DASTKS010000000
VERSION     DASTKS010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440627"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFQ35_28705"
     CDS             <1..380
                     /locus_tag="VFQ35_28705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934733.1"
                     /translation="DPRDDPARSADRRNYWEAWRAYARLAFGAPDSSGVCTQFEPEKI
                     PKTVRVVCARTKDGYSAEIAIAHAALNALRPRGAWSSLRFNLGVYERDAMSGPGTAIW
                     WQPDWRWEENVPGSGTFVSTRKN"
     gene            567..3254
                     /locus_tag="VFQ35_28710"
     CDS             567..3254
                     /locus_tag="VFQ35_28710"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HET9934734.1"
                     /translation="MTVESNATWYWLRPDRQQASGSLAELRNLLAARSLPRSTLVWAE
                     PWPEWLPASEVAALADAIPPAAPRPASEPPPRPSAPPLVRRNAPAPLHEPARSKAPSA
                     QPPKFDPAKREAANGSLKLEPPRANGALRPAPSLKAEAFLNSLSKKPSSAKVDSLRAE
                     PFTASDEVNAQTSARSDLPKLEARRSAAPKPLAPKSNLPKLEPRATQGDKVPPRAVAK
                     SDGAPAAEPRAEAPAVLPRPGDVSSALPGPVHGSPLPTPHEAAPALTGPVEGSGLAEP
                     AAASHADVAQLEGAPPVTRGEPPARPLAELDPELSQAVASVSMPEPIEAAPEVLPKVE
                     AASVARSSLDDLESKASELETVPLAVPVDTLREPAARDASTVEPKLDDDAKFEVSARD
                     IVKVEELPSTAVHPEEPSSPAAAFAEPSEAAPEGDRELLTLPAEHLVTEAPAAALHEA
                     APMPGEALGVADAKREELFASDYGVHGPASTPGFFPAQPRRALTNTDAPVELAASEEG
                     LVPPPGRRANEGGRGAKTALWAVSSVCAVLVLALGFTVRALRSRPLETAVQSSVTAPP
                     PKPEEPQSFQCSVLAAAAKLATSVERNIEPSVTPIDDARVAIGFASAPGEAEGIVVDL
                     GTLDVSQAFQEKADARVAQVVASAGQPPRFAVQREALDFASARVLETEPSSELGVAPE
                     ALVIRRGAEKKVLWPLPPGHGAEPKTARQQDGSYRVVLRRGGVAGDLVTGKIGADGNA
                     AGELSVIDAGVRMLGSPVVAANKSASFVVFAGRDQKTDPFRLRAASLSTGEPSPRAAD
                     LPIPTAEGNGALAPSLTPTADDRWLLQWTQGEVGRYRVFVQALSKELKPEGTAVAVSP
                     MGANAGQGVVRAFGDKALSLFVLPVPGHDELWGAVLTCR"
     gene            3251..4126
                     /locus_tag="VFQ35_28715"
     CDS             3251..4126
                     /locus_tag="VFQ35_28715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934735.1"
                     /translation="MKSHFRAGRLSIALFASVAAFGCAATGSNRPTEVAQGKSFRSGN
                     ADYDRFFDATHALQVKVGAASDELADARQHLTDALKVARSTGSPALAEGIKNELDRLN
                     RHGTHVRVDFVVPQPIEPSGTRAVLNASSKPRGEDANLLRQLESSVTRLLRLEAQMRV
                     ARRELDVLCSSAFRLESNLEASFSDRSQRALVTENLRDAERAITLMLGRAEDVQAPTA
                     DLLSRLAVAVGSPWRPAPLRPELESTEPRYARSQRAGDKTGGEPGRTEPASSGSAATG
                     SASSSEPVTRADFEP"
     gene            complement(4136..>4649)
                     /locus_tag="VFQ35_28720"
     CDS             complement(4136..>4649)
                     /locus_tag="VFQ35_28720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934736.1"
                     /translation="TCIDSAALSESLLSQLTTLTGCSALSLESSHANQTGTATFNADM
                     TFESVRTASYDATVAVPNSCITQGGFTLTCAQLNQLIKQFMAPQIGGVDCKDASAGCS
                     CKLSTPPQSLNDSGTYSTSGTKITSPITTETGDYCVQGDELHFIAVDPMSTGADGKQR
                     ILTDVVGRKR"
BASE COUNT          765 a         1555 c         1561 g          768 t
ORIGIN      
        1 tcgaccctcg tgacgatcct gctcgctctg cggaccggag aaattactgg gaagcgtggc
       61 gagcgtacgc tcgcctggcg ttcggtgcgc ccgattcgag tggcgtttgt acccagttcg
      121 agcctgaaaa gattccgaag acagtgcgtg tcgtttgtgc tcggacgaaa gacggttact
      181 ccgccgagat cgcgattgcg cacgccgctc tgaacgcgct gcgtccgcgc ggcgcgtgga
      241 gcagcctgcg tttcaatctc ggtgtctacg agcgtgatgc aatgagtggg cccggcacgg
      301 cgatctggtg gcagcccgac tggcgttggg aagaaaacgt cccggggtcc ggtacctttg
      361 tgtcgacccg caagaactga tttttgcggc cagaagaaat gcaaacggcg cttcgcagct
      421 cgcgtcggtc tcgttctgga ccaaggggcg cgagtaaatt tgtggcgagc ccttgagttt
      481 ggtggcaagt cgggagggtt ttcgggaatt gacacattgc gttctcccga aggagccgct
      541 gctgcggctc gaacaggatc gaagctttga ctgtcgaatc gaatgcaacg tggtactggc
      601 tccgacccga tcgccagcag gcttccggct ctctcgctga gctccggaat ctcttggcgg
      661 ctcgctcgct gccgcgctct acgctggttt gggccgaacc ttggcccgag tggcttccgg
      721 cgagcgaagt cgcagccctg gccgacgcca ttcccccggc cgcgcctcgc cccgcgtcag
      781 aaccaccgcc acgccccagt gctccgcccc tggttcggcg caacgcgccg gcccctttgc
      841 acgagcccgc gcgctcgaag gcgccctctg cacaaccccc gaaattcgat ccagcgaagc
      901 gcgaggcagc gaacggctcg ctcaagctcg agccaccgcg cgccaacgga gcgctgcgac
      961 cggctccttc gttgaaggcc gaggcgttcc tcaactcgct gtcgaaaaag ccttcgagcg
     1021 cgaaggtaga ctcgttacgc gcagagccat tcactgcgag cgacgaagtc aacgcccaaa
     1081 cgtccgcgcg gtcggacctg ccaaagctcg aagcgcgccg gagcgccgct cccaagccgc
     1141 tcgcgccgaa gtcgaatctt cccaagctcg aaccgcgcgc gacccagggg gacaaggtcc
     1201 cgccgcgcgc agttgcgaag tcggacggag ctccggcggc ggagcccagg gcggaagctc
     1261 cggcggtgct gccgcgaccc ggcgacgtgt cgtccgcgtt accggggccc gtccacggtt
     1321 caccgctgcc gacaccgcac gaggcagcac cggcgttgac agggccagtc gaaggttccg
     1381 ggctcgcgga gccagcagca gcttcgcacg ccgacgttgc gcagctcgag ggagcacctc
     1441 cggtgactcg aggcgagccg ccggcgcgac ctcttgccga gctcgacccc gagctgtctc
     1501 aggctgtggc gtcggtgtcg atgccggagc cgatcgaagc cgcgccagag gttttgccca
     1561 aagtcgaagc cgccagcgtt gcgcgctcga gtttggacga cctcgagagc aaagcatcgg
     1621 agctcgaaac ggtgccgctc gcggtgccgg tcgacactct tcgggaaccc gccgctcgag
     1681 acgcttccac cgtcgaaccg aagctcgacg atgacgcgaa gttcgaggtc tccgcgcgcg
     1741 acatcgtcaa ggtcgaggag ctaccgagca ccgccgtgca tcctgaggaa ccttcgagcc
     1801 cggctgccgc gttcgcagaa ccgagcgaag ccgcacccga aggcgaccgt gagctcttga
     1861 cgcttccagc tgaacacctc gttaccgagg caccggccgc ggcgttgcac gaagccgcgc
     1921 ccatgcccgg cgaagcgctc ggcgtagccg atgcgaaaag ggaagagctc ttcgcgagtg
     1981 actacggggt gcacgggccc gcgtcgacgc ccggattttt cccggcccag ccacggcgcg
     2041 cgctcacgaa caccgacgcg cccgtcgaac tcgcggcctc ggaagaaggg ctcgtaccgc
     2101 cgcctggccg tcgtgccaac gagggggggc gcggcgcgaa gacggcgctg tgggcggttt
     2161 cctccgtttg cgcggtgctc gtcctggcgc tcgggttcac ggtgagagcg ttgcgctcgc
     2221 gcccactcga gacggcggtg caaagcagcg taacggctcc gccccccaaa cctgaagagc
     2281 cgcagtcgtt ccaatgtagc gtccttgcgg ccgcggcgaa gcttgccacg agcgtcgagc
     2341 gcaacatcga gccgagcgtg acgccgatcg acgacgctcg cgtggccatc gggtttgcgt
     2401 cggcgcccgg cgaagccgag ggcatcgtgg tcgatctcgg tacgctcgac gtcagccaag
     2461 ctttccaaga gaaagccgac gcgcgtgtcg cgcaggtcgt cgcgagcgct gggcagccgc
     2521 cgcgcttcgc cgtgcagcgc gaggctctcg atttcgcttc cgcgcgcgtt ctcgagaccg
     2581 agccttcgag cgagctcgga gtcgcgccgg aggctttggt catccgccgc ggagcggaaa
     2641 agaaggtgct ctggccgctc ccgcccggtc acggtgccga acccaagacc gcgcgtcagc
     2701 aggacggtag ctaccgcgtg gtgctgcggc gcggcggcgt cgcgggggat ctcgtgaccg
     2761 gcaagatcgg tgcggacggc aacgccgccg gcgagctcag cgtgatcgat gccggcgtgc
     2821 gcatgttggg ttcgccggtg gttgccgcca acaagagcgc aagcttcgtc gtcttcgcgg
     2881 gccgcgatca aaagacggat cctttcaggt tgcgagctgc ttcgctgagc acgggtgaac
     2941 ccagcccgcg cgcagccgac ctgccaatcc ccaccgcgga aggaaacggc gcgctcgccc
     3001 cgtctctcac tccgaccgct gacgatcgct ggctactcca gtggacgcaa ggcgaggtgg
     3061 gtcggtatcg agtgttcgtg caagccctga gcaaggagct gaagccggaa ggaaccgcgg
     3121 tggcggtttc gccgatggga gcgaacgcgg gacagggcgt ggttcgagca ttcggagaca
     3181 aagcattgtc gctcttcgtg ttgccggtgc cgggtcacga cgagctttgg ggtgccgtac
     3241 tcacatgtcg atgaaatcgc attttcgcgc aggccgactc agcatcgcct tgttcgcctc
     3301 cgtcgccgcg ttcggttgcg ccgcgactgg cagcaatcgc ccgaccgagg tcgcgcaagg
     3361 aaagagcttc cgctccggga acgccgacta cgatcgcttc ttcgacgcga cccacgcgtt
     3421 gcaggtgaag gtgggggccg cttccgacga actcgccgac gcgcgccagc acctcaccga
     3481 cgcgctgaag gtcgctcgca gtacggggtc gcccgccctc gcggagggaa tcaagaacga
     3541 gctcgatcgt ttgaatcggc acggcacgca cgtgcgcgtc gacttcgtcg tgccgcaacc
     3601 gatcgagccg agcggcacgc gcgcggtgct caacgcttcc tccaagcctc gtggggagga
     3661 cgcgaatctc ttgcggcagc tcgagagctc ggtgacaagg ctcctgcggc tcgaagctca
     3721 aatgcgcgtc gctcgccgcg aactcgacgt cctctgctcg agcgcgtttc ggctcgaaag
     3781 taacctcgaa gcgagcttct cggaccggtc gcagcgcgcg ctcgtgaccg aaaacctacg
     3841 cgacgccgag cgcgcgatca ccctcatgct ggggcgcgcg gaagacgtgc aagcaccaac
     3901 cgcggatttg ctgagcaggc tcgcggtcgc cgtcggttcg ccctggcggc cagcccccct
     3961 gcgcccggag ctcgagagca cggagccgcg ctacgcccgc tctcagcgcg ccggggacaa
     4021 gaccgggggc gagccgggcc gcaccgagcc cgcttcgagc ggctccgccg cgactggctc
     4081 cgcttcttcg tccgagccgg tgacgcgcgc cgatttcgag ccttgacgct cgggtttacc
     4141 gtttcctgcc gacgacgtcg gtgaggatcc gctgcttgcc gtcggcgccg gtcgacatcg
     4201 ggtccaccgc gatgaagtgg agctcgtcgc cttggacgca gtagtcgccg gtctcggtcg
     4261 tgatcggcga cgtgatcttg gttcctgacg tcgagtaggt tccggagtcg ttcaacgact
     4321 gcggcggggt cgaaagcttg cagctacatc ccgcgctcgc gtccttgcag tccacccctc
     4381 cgatttgcgg cgccatgaac tgcttgatca gctggttgag ctgcgcgcag gtgagggtga
     4441 agccgccttg ggtaatgcag ctgttcggaa ccgcgacggt ggcgtcgtaa gacgccgtgc
     4501 gcacgctctc gaacgtcatg tccgcgttga aggtcgccgt gcccgtttga ttcgcgtgac
     4561 tcgactcgag gctgagcgcg ctacagcccg tcaacgtggt gagctggctg agcaggctct
     4621 cgctcaacgc cgctgaatcg atgcaggtg
//
LOCUS       DASTKS010001340         7554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1112345, whole genome shotgun sequence.
ACCESSION   DASTKS010001340 DASTKS010000000
VERSION     DASTKS010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112345"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFQ35_28725"
     CDS             <1..193
                     /locus_tag="VFQ35_28725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934737.1"
                     /translation="SFTPVQESVIREIITHRAQFDARDEDGVGFGGAQVGNNSAADVG
                     LCEPVVADYASYQELLAAL"
     gene            complement(332..859)
                     /locus_tag="VFQ35_28730"
     CDS             complement(332..859)
                     /locus_tag="VFQ35_28730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9934738.1"
                     /translation="MHAEQGQRHVNIRRAEPSDVHAVVELIRAWALEVFAREAEVTPE
                     ALLGDGFGSVLEFFVAEETTGELCGFAAWEKTYDVITGRRGGALLGQFIAPTTRGLGF
                     GDRLLHAVANEVRAIGGAFLSGLGEAHHDLTAAASLVPPPLHVAGGATKKRSAADLSP
                     HGEHMHFLSALRPET"
     gene            complement(956..2272)
                     /locus_tag="VFQ35_28735"
     CDS             complement(956..2272)
                     /locus_tag="VFQ35_28735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934739.1"
                     /translation="MNRLARRAFLATFLAANASLALGRRPYGGTLRVLVPWGMNRLDP
                     HELDDPIAALFAHLTCDAVFAIDANGRPYPTLARALPEPIAGGARVRLRPGLVSAQGH
                     ALSALDLKASIERSQRRGGAALFSSISNLKRVPGDTLSLDFLGERPEELAVRLANPLA
                     ALLPKTFSPLEPDGTGAYKARLGPGELVLRRNENAARGAAFLDAVEAHTVSDLSDALR
                     AFENGNVDVGWLGSGLHQPRSGAIPFVGAAYGWVVLRSGKKPRQWGAPGVAQQLIDGI
                     PPERLRHLGLKNLPERASPSEGWGGGPVECLVPDDAPQLRLTAQTLAAALSRPGHEVT
                     AVALPRTECAKRRQTGDFALLVDFVRVIGAPGPLTQLALLSAENPDLAKRPPRASDFN
                     PRSIAKSLALGVVGELWVEGAHAPAFRGLAGWQLGDAYRLPRENSP"
     gene            complement(2269..3555)
                     /locus_tag="VFQ35_28740"
     CDS             complement(2269..3555)
                     /locus_tag="VFQ35_28740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934740.1"
                     /translation="MKVRWLLLLAAVAFPRAARADGRGAPCAERQSSALGQIACELAH
                     NLPTPTQPMRVMVHAITSPLRMERADDLANRLARAVAGELGPNARVEPPTAGPARIDS
                     LVIDAELTRERFSVTAELFTGVAKFWDRFRPGSRGTTARAHAAHAVDAELASYLPPVP
                     LVLTRVDKARVDEPSVALACGDVEGDGGLAIVSVGRRRIQVGRVARGRFDVTHSKAWS
                     ELSDIAPRPLREPLASAYVDANHRVQVGISDRSEALSLDRELLPTARFRATLPWPNAG
                     CVPFVPGGVGEARGRCDAPPPRNPGGPLDALAGARLARRDGTTFDVFAGRRTNAELSL
                     EVGGTRFEAGTSGAQLALADLDSDGSAELISSLDTLEPSGDAVVVRTILADGKLREAF
                     RIPVPNGVRALAACPAWGTGLAPILIATGDEVWVAR"
     gene            3740..4420
                     /locus_tag="VFQ35_28745"
     CDS             3740..4420
                     /locus_tag="VFQ35_28745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HET9934741.1"
                     /translation="MRLAAALVGGALFWMGGGTPEARAQCVDETLKQQLIGQRAYRGV
                     VPRLFQKALRHELSPMGGWYAGDISDGAPIYGGAYTFNFTEDLGLEAAYFRSRQSFAL
                     LRAIDQRQQGLVDIVRSEDNPIQFFVGSLVWSLAYGKVRWMGGAISRFDFNIAIGGGA
                     TVEPGAIGLTGSGGFGMKFYLTEWLALRLDVRDYVREQKRVQLGVEKIVNDITAMGGA
                     SVFIPFTN"
     gene            4432..5178
                     /gene="gpmA"
                     /locus_tag="VFQ35_28750"
     CDS             4432..5178
                     /gene="gpmA"
                     /locus_tag="VFQ35_28750"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HET9934742.1"
                     /translation="MYKLVLLRHGTSQWNQENRFTGWVDVPLSPAGIEEAKNAAKLLR
                     EAGLSFDVAYTSVLKRAIKTLWIVLEENDLMWIPVIRSWRVNERMYGGLQGLNKSETT
                     AKHGEAQVKIWRRSYDVPPPPIEDDNQYSPKKDPRYKKLSPSELPKTECLKDTVARFL
                     PLWEKEIAPAIKRGERVLIAAHGNSLRALVKHLDNVSDDEIVELNIPTGIPLVYELDE
                     NLRPIKPRYYLGDAAAAEAAAKAVASQGKQ"
     gene            5357..6142
                     /locus_tag="VFQ35_28755"
     CDS             5357..6142
                     /locus_tag="VFQ35_28755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934743.1"
                     /translation="MRNRWVVSLCLAVMLGTPLSFAQGKRGKPAAPAKAPPAAAPARP
                     IAEEKERDKDKPPAPAEEKPAAEAPPGSDPELGAPPPKAAEGASTTPISPLTPAPDEF
                     VQGPPPAAPADFDKLLGDIAALRSRVAAVTTTLFKSRLRVVLESRADDARIEALAITL
                     DDGVVFAAPERFSADDDRVVYEHAVAPGSHVLGIDVERYDARARQYRTWQNSRFSVVV
                     PESQLVEAHFRIEDSSDMASDFPSDQDGEYKLVVRLRARVTSK"
     gene            6139..>7554
                     /locus_tag="VFQ35_28760"
     CDS             6139..>7554
                     /locus_tag="VFQ35_28760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934744.1"
                     /translation="MNALSHRLALVASLLAWALPAAAQPDAVPAAEGAPAIQEPKAAA
                     SAESAPAKPPVEDTRGQAFRAYQTALEARKLAATAPLSIQRIEKDLAGIEEKLLSGRR
                     DEAIADLTYIVESPRFEPFASSEPGRAAVFLLGDALGRGGAYEPARGYLRRLLSSPTT
                     DTWFRRAVGSSVDLALQSDKPQVFLADLDAVESRAPEEAQGEIEYLRGRVAELEGAPD
                     RALAAFAKVSERSRFWAQATYLSGVIEVDRRNFKRSEELFCKVADPKRSPRRAAVFGG
                     SDFFRVRDLSRLGLGRVAHEQYRFDDARYYYYLVPSDSEHLPEALYETATTRYEAKDY
                     KAAREAIDDLRRLQQSHPYEDEAYILDAYIDLALCRFPEADKKLIEFLRRYEPARDAA
                     RKIGQDEAAMRRLVESVRKGADPAGAGLGVPDDTARALGAVLRVDSGYARVTSRLAQV
                     EHELSGLRGTLGDLDEARRKLA"
BASE COUNT         1286 a         2476 c         2594 g         1198 t
ORIGIN      
        1 ctcgttcacg cccgtgcaag aaagcgtgat tcgagaaatc atcacccatc gggcccagtt
       61 cgacgcgcgc gacgaggacg gcgtgggttt cggtggagcg caggtcggta acaactcggc
      121 cgccgacgtc ggcctatgcg agcccgtcgt tgccgactac gcttcgtacc aagagctgct
      181 cgcggcgctg tagcgctcgc ggctcgagtg ggcgcggcac caacacgttg cggcgctgag
      241 gacccgcgaa gggacaagtc atactgcgcg agccgatcct cttgctgcgg cgcgcgggct
      301 cgtggaggag gggaggtgtc gaagtttacg tctacgtctc cggcctgagc gcggagagaa
      361 agtgcatgtg ttcgccgtgg gggctcagat ccgcagcgga gcgcttcttg gtggcgccgc
      421 cggctacgtg gagcggtgga gggacgaggg acgcggcggc ggtgaggtcg tggtgcgctt
      481 cgccgagccc agacaagaaa gcgccgccta tcgctcgcac ttcgtttgcg accgcgtgca
      541 gcagcctgtc gccgaagccg agtccgcgcg tggtaggcgc gatgaactgc ccgagcaatg
      601 cgccgccgcg gcgaccagtg atgacgtcgt aagtcttttc ccacgccgcg aacccgcaga
      661 gctcacccgt ggtctcttca gcgacgaaga actcgagcac ggagccgaac ccgtccccca
      721 gcagcgcctc gggcgtgacc tcggcctccc gcgcgaacac ttcgagcgcc caggctcgga
      781 tgagttcgac gacggcgtgg acgtcggaag gttcggcgcg gcgaatattc acgtggcgtt
      841 gtccctgttc ggcgtgcatc gccagttgat ggtagcgagc tttcgatgaa gggacgagcg
      901 ggatgaattc gcttggagtg gtgaatcaaa gtcgccacgt ttgggcgaga tggttttagg
      961 gggagttctc gcggggcagg cggtaggcgt cacctagctg ccacccggcg agtccgcgaa
     1021 atgccggcgc atgggcgccc tccacccaca gctcgccgac gactcccaag gcgaggctct
     1081 tcgcgatgct acgcgggttg aagtccgagg cgcgcggcgg cctcttggca agatctgggt
     1141 tttcggcgga gaggagcgcg agctgggtca aaggacccgg cgcgccgatg acgcgaacga
     1201 aatcgacgag cagtgcgaag tctccagtct gacgtcgctt cgcgcactcg gtccgcggga
     1261 gcgcgacggc tgtcacttcg tgccccggcc ggctcaaagc ggcagcgagg gtttgcgcgg
     1321 taagacgcaa ctgcggagcg tcgtccggca cgaggcactc gacggggccg ccaccccagc
     1381 cttcggaggg cgacgcgcgc tctggaagat tcttcaaccc gaggtggcgc aaccgctcgg
     1441 ggggaattcc gtcgatgagc tgttgagcaa ctcccggagc gccccactgc cgcggctttt
     1501 tgccgctgcg caatacgacc caaccataag cggcgccaac gaacggaatc gcgcccgagc
     1561 gcggctggtg caagccgctg ccgagccagc ccacgtcgac gttgccgttc tcgaaagcgc
     1621 gcagtgcatc cgacagatcc gataccgtgt gcgcctcgac ggcgtcgagg aaggctgcgc
     1681 cgcgcgccgc gttctcgttc cttcgcagca cgagctcgcc cgggccgagg cgcgccttgt
     1741 aggcgccggt cccgtcgggc tcgagcggcg aaaaagtctt cggtaagagg gcggcgagcg
     1801 gatttgcgag tcggactgcg agctcttcgg ggcgctctcc caagaagtcg agcgacaggg
     1861 tgtcgcctgg cacgcgcttc agattcgaaa tcgacgagaa caacgcggcg ccaccccgac
     1921 gttggctccg ctcgatcgac gctttcaagt cgagtgccga gagcgcatgg ccctgcgcac
     1981 tgactagccc ggggcgcagc cgaacgcgcg cgccgccagc gatgggctcg ggcagagcgc
     2041 gggcgagcgt cgggtagggg cggccattgg cgtcgatcgc aaacaccgca tcgcaggtga
     2101 ggtgagcgaa gagtgccgcg atcggatcat cgagctcgtg tggatcgagt cgattcatcc
     2161 cccatggcac cagcacccgg agcgtgccgc cgtacgggcg acggccgagc gccaagcttg
     2221 cattggcagc caagaacgtc gcaaggaagg cgcggcgcgc gagtcggttc atcgcgcgac
     2281 ccagacttcg tcgccggtcg cgatgagtat gggggcgagt ccggttcccc acgcggggca
     2341 cgccgcgagc gctcgcaccc cgttcggcac gggtatgcga aacgcttcgc gcagcttgcc
     2401 gtccgcgagg atggtgcgca ccacgaccgc gtccccgctg ggctccaaag tatctaggga
     2461 ggagatgagc tccgcgctgc cgtcgctatc gagatccgcg agcgcgagct gcgcgcccga
     2521 agtccccgct tcgaagcggg tgccgccgac ctcgagcgag agctctgcgt tagtccgacg
     2581 gcccgcgaac acgtcgaacg tcgttccgtc gcggcgcgcg agccgcgcac cggcgagcgc
     2641 atcgagcggg ccacccgggt ttcgcggcgg gggcgcgtcg cagcgcccgc gcgcttcgcc
     2701 gactccgccg gggacgaacg gaacgcagcc agcgttcggc caagggaggg tggcgcgaaa
     2761 acgcgcggtg gggagaagct cgcggtcgag cgagagcgct tccgaacggt cgctgatgcc
     2821 gacttgaacg cggtgattgg cgtcgacgta ggcgctcgcg agtggctctc gcaacgggcg
     2881 cggcgcgatg tccgagagct cgctccaagc tttactgtgc gtgacgtcga agcggccgcg
     2941 cgcaacgcgg ccgacctgaa tgcggcgccg gccgacgctc actatcgcga gccctccatc
     3001 cccttccacg tcgccgcacg cgagcgcgac gctgggctca tcgacgcgcg ctttgtcgac
     3061 gcgcgtgagc acgagcggga cgggcggtaa gtaggatgcg agctccgcgt cgaccgcgtg
     3121 cgccgcgtgc gctcgcgcgg tcgtgccgcg gctaccggga cggaagcgat cccaaaactt
     3181 cgcgacgccc gtgaagagct ccgcggtcac gctgaagcgc tcccgagtga gctccgcatc
     3241 gatgacgagc gagtcgatgc gcgctgggcc cgcggtgggc ggctccacgc gagcgttcgg
     3301 accgagctca ccggccacgg cgcgtgcgag gcgattggcg agatcatcgg cgcgctccat
     3361 tcgcagcggg gacgtgatcg cgtgaaccat cacacgcatc ggctgagtgg gagtgggtag
     3421 gttatgcgcg agctcgcaag cgatctgtcc gagcgcgctc gactggcgct cggcgcacgg
     3481 cgcgccccgg ccgtcggccc gagccgccct cgggaacgcg acggcagcga gcaacagcag
     3541 ccaacgaacc ttcatttttg ccgcatacga caggccgtcg ttcccaacca catcgatctt
     3601 gaaccctggg gggcattgcc ccgaggcgga acgcctgtcg gcagaagcca cacctagctg
     3661 aggtcaccaa ctgcagaatc gccccggatc ccggccactt atgatactcc cgtgggtcga
     3721 gttggctaga ggtactgctt tgcggctcgc ggcggccttg gtgggcggcg ccctcttttg
     3781 gatgggcggg ggcactcctg aggcgcgcgc gcagtgcgtc gacgagacac ttaaacagca
     3841 actcatcggg cagcgcgctt accgcggcgt cgtgccgcgc ctgtttcaga aggcgcttcg
     3901 gcatgagctc tccccgatgg gtggctggta cgccggcgac atctccgacg gcgcgcccat
     3961 ctatggaggg gcgtacacct tcaacttcac cgaggatctg ggcctggagg ccgcctactt
     4021 tcgctcgcgc cagagcttcg cgcttctccg cgcgattgat caacgtcagc aggggctggt
     4081 cgatatcgtc cgttccgaag acaaccccat tcagttcttc gtggggagct tggtttggtc
     4141 gctcgcctac ggcaaggttc gctggatggg cggcgcgatc agtcgtttcg acttcaacat
     4201 cgccattggt ggaggagcga ccgtggagcc cggcgcgatc ggcctgaccg gctcgggtgg
     4261 gttcgggatg aagttctatt tgaccgaatg gctcgcgctg cgtcttgacg tccgagacta
     4321 tgtgcgcgag caaaagcggg tgcagctcgg cgtcgagaag atcgtcaacg acatcaccgc
     4381 gatgggcggc gccagcgtct tcatcccctt taccaactga gagcctcccc catgtacaag
     4441 ctcgttctgc tccgccacgg caccagccag tggaaccagg aaaaccgctt caccggctgg
     4501 gtcgacgtcc cgctaagccc cgccggtatc gaagaagcca agaacgccgc gaagctgctg
     4561 cgcgaggccg gcttgagctt cgacgttgct tacacctccg tgctgaagcg cgcgatcaag
     4621 accctctgga tcgtgctcga ggaaaacgac ttgatgtgga tcccggtgat ccgctcttgg
     4681 cgtgtcaacg agcgcatgta cggcggtctc cagggcttga acaagtccga gacgacagcg
     4741 aagcacggcg aggctcaggt caagatctgg cgtcgcagct acgacgtccc cccgcctccc
     4801 atcgaggacg acaatcagta ctcaccgaag aaggatcctc gctacaagaa gctgagcccg
     4861 agcgagctgc cgaagaccga gtgcttgaag gacacggtcg cgcgcttcct cccgctttgg
     4921 gagaaagaga tcgctccggc catcaagcgc ggcgaacgcg tcctcatcgc tgcccacggc
     4981 aacagcttgc gagcgctggt aaagcatctc gacaacgtga gcgacgatga gatcgtcgag
     5041 ctcaacatcc cgacggggat cccgctcgtt tacgagctcg acgagaacct gcgcccgatc
     5101 aagccgcgct actacttggg cgatgcggcc gcggcggagg ctgccgcgaa ggcggttgct
     5161 tctcaaggca agcagtagac tagcgattga gcgtcggtcc gcgcctgggg cgcggcacgc
     5221 gacgatgagc gccggagagt cgggggcact ccggcgctcg cgcgtcgccg atgtgagaaa
     5281 aggtaggggc cgaacgagcg cggccggaac gagatgctga cgcccttcgg tggggcgtgt
     5341 tagcgtcgtt cgtcgcatgc gaaatcgttg ggtcgtttcg ctgtgtctgg ccgtgatgct
     5401 cggcacgccc ctttcgtttg cacaaggcaa gcgaggcaag cccgcggcgc cggccaaggc
     5461 acctcctgca gccgcgcccg cgcgtccgat cgccgaagaa aaagagcgcg acaaggacaa
     5521 gccgccggcg cccgctgaag aaaagcccgc tgcggaggct ccgcccggct cggatccgga
     5581 gctcggcgcg ccaccgccga aggcggctga aggcgcgtcc acgaccccca tttcgccgct
     5641 cacgcctgcg ccggacgagt tcgtccaagg gccgccgccg gcggcgcccg ccgacttcga
     5701 caaattgctc ggtgacatcg ctgcgctccg cagccgcgtg gccgcggtga cgacgactct
     5761 cttcaagagt cgtttgcgcg tcgtgctcga gagccgcgcc gacgacgcgc gcatcgaagc
     5821 gctcgcgatc acgctcgatg acggagtggt gttcgccgca cccgagcgct tcagcgccga
     5881 tgacgatcgt gtcgtgtacg aacacgcggt cgcgccgggc agtcacgttc tcggtatcga
     5941 cgtcgagcgt tacgacgctc gagcgcgtca gtaccgcact tggcaaaatt cgcgcttctc
     6001 ggtcgtcgtg cccgaaagtc agctcgtcga ggcgcacttc cgcatcgagg acagctcgga
     6061 catggcgagc gacttcccga gcgatcaaga cggcgaatac aagctcgtcg tgcgcttgcg
     6121 ggcgcgcgtg acgtcgaaat gaacgcgctg tcgcaccgtc tcgcgctcgt cgcgtcgttg
     6181 ctcgcttggg cgttgcccgc cgcggcccag cccgacgcgg ttccggctgc ggagggggcc
     6241 cccgccattc aagagccgaa agcggctgca agcgccgaga gcgcgcccgc caaacctccc
     6301 gtcgaggaca ctcgaggaca agcctttcgc gcttatcaga ccgcgctcga ggctcgaaag
     6361 ctcgcggcca ctgcgccgct cagcatccaa cgcatcgaaa aagatcttgc cggcatcgag
     6421 gagaagctgc tgtctggtcg ccgcgacgag gccatcgcgg atttgactta catcgtcgag
     6481 tcgccgcgct tcgagccgtt cgcgagctcc gagcctggac gcgcggcggt gttcttgctg
     6541 ggcgacgcgc tcggccgtgg gggggcgtac gaacccgccc gcggctatct gcggcggctc
     6601 ctgagctcgc cgaccaccga tacgtggttc cggcgcgcgg tcgggagctc ggtcgatctc
     6661 gcgttgcaga gcgacaagcc gcaagtattt ctcgccgatc tcgacgccgt cgagtcgcgc
     6721 gctcccgaag aagcgcaggg cgaaatcgag tacctgcgcg gtcgagtcgc cgagctcgag
     6781 ggcgcacccg atcgcgctct cgccgcgttc gcgaaggtga gcgagcgctc ccgcttctgg
     6841 gctcaagcga cgtacctctc cggcgtcatc gaggtggatc gcaggaactt caagcgcagc
     6901 gaagagctgt tctgcaaagt tgccgacccg aagcggtcgc cgcggcgcgc ggccgtattc
     6961 ggcggcagcg acttcttccg cgtgcgcgat ctgtctcggc tcggtctcgg gcgcgtcgcg
     7021 cacgagcagt accgcttcga cgacgcgcgc tactactact acctcgtgcc gagtgattcg
     7081 gagcacctgc cggaggcgct ctacgaaacg gcgacgacgc gctacgaggc caaggactac
     7141 aaggccgcgc gcgaggcgat cgacgatctg cgccgcttgc agcagagcca cccgtacgag
     7201 gacgaagcgt acatcctcga tgcgtacatc gacctcgcct tgtgtcgctt cccggaggcg
     7261 gacaagaagc tcatcgagtt tctgcggcgc tacgagccag cgcgcgacgc cgcgcgcaag
     7321 ataggccaag acgaagcggc gatgcgtcgc ttggtcgaga gcgttcgtaa aggagcagac
     7381 cccgccggtg ctgggctcgg cgtgccagac gacaccgcgc gcgcactcgg tgcggtgctg
     7441 cgcgtcgact cgggctacgc gcgggtgacg tcgcggctcg cgcaggtgga gcacgagctc
     7501 tctggcttgc gcggcacgct cggcgatctc gacgaggctc ggcgaaagct cgcg
//
LOCUS       DASTKS010001341         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1559990, whole genome shotgun sequence.
ACCESSION   DASTKS010001341 DASTKS010000000
VERSION     DASTKS010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559990"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VFQ35_28765"
     CDS             <1..1160
                     /locus_tag="VFQ35_28765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9934745.1"
                     /translation="RAREEAVVIGALFGTIVANAALVAYTYVGYPILVGACAALRGER
                     KPPPPPKDEEVPSISVLLPVYNGASDLPAKVESLLNQDYPADRLELLIYCDGCSDDSQ
                     SVAEELAAASANRTRIRVVASRDRKGKPAALNALAALAEGELLLMNDVRQPLSRNSAR
                     ALAAEFRDPKVGCATGVLVLSGNAASGAYWRYEDWIRSRESRFRGVVGMTGAIAMMRR
                     SDFVPLPEEVILDDVWIPMRLVLLKKRVVSVREAEAYDAAFADAREFRRKVRTLAGNY
                     QLFALLPDLLSPFKNPLWLETVSHKVLRLAVPWSLGALLAASAGTMLGGGAAALVGGT
                     LFGGQVLFYVAAAVGPRAGRIGTLARTFVVMNGAAALGLWRYATGRQQVTW"
     gene            complement(1286..1597)
                     /locus_tag="VFQ35_28770"
     CDS             complement(1286..1597)
                     /locus_tag="VFQ35_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9934746.1"
                     /translation="MQFSVNTEGAKVVVRIRGEFDANSAPSVRAQIDAIVESRPSHVE
                     VDLSSLRLIDSTGVGGIVSLFKRTRAYGGQFNVTGVQGQPRSIFRLLRLDHVFGLDET
                     S"
     gene            2065..3855
                     /locus_tag="VFQ35_28775"
     CDS             2065..3855
                     /locus_tag="VFQ35_28775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9934747.1"
                     /translation="MDTSLTRLEPPSSSQQEPPSIRLECFLEIEHSERGQLATFGLAG
                     NRQLIGRTPDLPICLDHDGVSRLHAALIRDPYDRWWIHDLGSTNGTYVNGSAVTQRLL
                     NPGDTIQIGPYRMTLKVPGDPIPQERPKRPGTSRPRGPVSEMASTTRLPAVTADRLNA
                     ALSREAVGFARSLLRLEDPQLRLKQLCEHAVNDRIPGLWACVFRLSRESTSGLVYQAV
                     TRPGIGSSFAPAPRLIEALCREHAQSFLNDAGPHILGRQHGLAGNLVACVLDLSPVGV
                     DLFFVELTADCDADEWTPLLALLAESYGNANEFWQTRRQFSVNAAVQRELEMAQQLQD
                     SLIPRLPAVAGLELSIGYHPSRWVGGDYADAVRMPDGRLLIAIADVCGKGLQAALVAS
                     SLHTLVHVLVEVCDSLAQLVVHMDAHLCRYLPGESFVTMVLCALDVRTGELECLNLGH
                     LPPLLVGEGGSVRPLQAAENLALGMMPFTPRSEKYQMDRDEVLLLYTDGLTELENGRR
                     PGEEPAPPDTGLLAGALGGCVQIIHSMQGADINAIRDRMSQSVRIQLDLTMASDDAAF
                     ILARLQNRQHLLPEARNVTVAPPPPALKPR"
BASE COUNT          643 a         1359 c         1288 g          694 t
ORIGIN      
        1 ctcgggctcg tgaggaggcg gtcgtgatcg gcgcgctttt cggaaccatc gtcgccaacg
       61 ccgccctcgt cgcgtacacg tacgtcggct acccgatcct ggtgggcgca tgcgcggccc
      121 tgcgtggtga acgcaagccg cctcccccgc cgaaggacga agaggtcccg agcatcagcg
      181 tgctcttgcc cgtgtacaac ggcgcgtcgg acttacccgc caaggtcgag agcctgctga
      241 accaagacta cccagccgat cggctcgagc tcctcatcta ctgcgacggt tgcagcgacg
      301 acagccaaag cgtcgccgaa gagctcgccg ccgcgtccgc gaaccgcacg cgcatccgcg
      361 tcgttgcttc tcgagaccgc aagggcaagc cggcggcgct gaacgcgctc gcggccctcg
      421 ccgagggcga actgttgctg atgaacgacg tgcgccagcc cttgtcgcgc aactcggcgc
      481 gggcgctcgc ggcggagttt cgcgatccga aggttggctg cgccaccggc gtcctcgtac
      541 tcagcgggaa cgcagcgagc ggcgcgtact ggcgctacga ggattggatc cgctccaggg
      601 agtcacgctt tcggggagtg gtgggcatga ccggcgccat cgcgatgatg cgccgttccg
      661 atttcgtgcc cctgcccgag gaggtgatcc tcgacgacgt ttggatcccg atgcggctcg
      721 tcttgttgaa gaagcgagtc gtctcggtgc gcgaagcgga ggcgtacgac gccgcgttcg
      781 cggacgcgcg tgagttccgt agaaaggtcc gaactctggc cggcaactac cagctgttcg
      841 cgctgcttcc ggacctgctc tcccctttca agaacccgct ctggctcgag accgtttcgc
      901 acaaagtctt gcgcttggcc gtgccgtggt cgctcggtgc gctcctcgcc gcgtcggcgg
      961 gaaccatgct gggcggcggt gcggctgcct tggtcggcgg cacactgttc ggcgggcaag
     1021 tcctctttta cgttgccgcg gcggtgggcc cccgcgccgg tcgcatcgga acgctggctc
     1081 gcacgttcgt cgtgatgaac ggagcggcag cgctcggcct ctggcgctac gccaccggtc
     1141 gccaacaagt cacctggtaa cgcgcagcaa ctcgacgacg agccggtagg aaacagcgac
     1201 ctccggctca accggtttcg gcaggcttag tcggttcaac cgtttcggca ggatcagccg
     1261 gcaggctcag ccggctaagg cgggctcagc tggtctcgtc gagcccaaac acgtggtcga
     1321 ggcgcaacag ccgaaaaatc gaacggggct ggccctgaac accagtcacg ttgaactggc
     1381 cgccgtaggc gcgcgtcctc ttgaacaggg acacgatgcc gccgacgccg gtgctgtcga
     1441 tgagccgcaa cgaggagagg tcgacctcga cgtgactggg cctcgattcg acgatggcat
     1501 cgatctgggc gcggaccgag ggggcggagt tagcgtcgaa ctctccgcga attctgacca
     1561 ccaccttggc cccttccgta ttgacagaga actgcatttc cgccgtctat cgcctatagc
     1621 tggaagtcca cgataaccga agtcggcgga cacgggtaga ctcatttcgc cttttgccga
     1681 gctcgaaggt ccgagcggac ccgcacgttc tgcacggggt cttcctcggc ttacagcggt
     1741 cgcccgtgcc gcgacgccgc gccgcctgat ctggctgtca ctcgaaggcc cgccactctc
     1801 cgcgcgtttc cgaacgcggc gagtcaatgc cccgctcgag acgcccgcaa accttggccc
     1861 tgccgtttcc caatgagtcg aaggtgcgta tggaccccgt ttgccccatc acgcgatttc
     1921 taccaggctg cacacgaccc gaaagagctc gggccgtccg gcgcaaattt tgtcgcgttg
     1981 tcgccgtggg agagtcgcgg ccgttcatga aaagtcggcc ccgtgcttgt cggatggcgt
     2041 gcccgcgcgt aagagtggct agcgatggat acgagtctca cgcggcttga accgccgtcg
     2101 agctcgcagc aggagccccc tagcatccgg ctggagtgct tcctcgagat cgaacactcc
     2161 gagcgagggc agctcgcgac gttcggcctc gccggcaatc gtcagctcat cggtcgcacg
     2221 ccagatctgc cgatctgttt ggatcacgac ggcgtttcgc ggctacacgc cgcgctcatt
     2281 cgcgaccctt acgaccgctg gtggatccac gatctcggca gcaccaacgg cacctatgtg
     2341 aatggcagcg ccgtcacgca gcggctcctg aaccctggag acaccatcca gatcgggccg
     2401 taccggatga cgctcaaagt cccgggcgac ccaatcccgc aggagcgtcc gaagcgaccc
     2461 gggacctcgc ggccccgggg acccgtgagc gaaatggcgt cgacgacgcg cctgcccgcg
     2521 gtcaccgccg accggctgaa cgcagcgttg tcacgggagg cggtcggctt tgctcgctcg
     2581 ttgttgcgcc tcgaagaccc gcagcttcgg ctgaagcagc tctgcgagca cgccgtgaac
     2641 gaccgcattc ccgggctgtg ggcttgcgtc ttccggctca gccgcgagag tacctccggg
     2701 ctcgtgtacc aggccgtaac gcgccccggc atcgggagct cgttcgctcc ggcgccacgt
     2761 ctgatcgagg cgctttgccg agagcacgcg cagtcgtttc tgaacgacgc gggaccgcac
     2821 atcctcggtc gtcaacatgg gctcgccggg aacctcgtcg cctgcgtcct cgatctttca
     2881 ccggtcggcg tcgatctgtt cttcgtcgag ctcaccgccg actgcgacgc cgacgagtgg
     2941 acgccgctgc tcgcgctgct cgcggagtcg tacggaaacg cgaacgagtt ctggcaaacg
     3001 cgccgccagt tcagcgtgaa cgcagccgtg cagcgcgagc tcgaaatggc gcagcagctt
     3061 caagacagct tgattccgcg tctcccggcc gtcgctgggc tcgagctttc gatcggttat
     3121 cacccctcac gttgggtcgg cggagattac gctgacgcgg tgcgcatgcc cgacgggagg
     3181 ctgctcatcg ccatcgccga tgtttgtggg aagggcctac aagcggcgtt ggtcgcctcg
     3241 agcttgcata cgctcgtgca cgtcctggtg gaggtctgcg atagcctcgc gcagctcgtc
     3301 gtccacatgg acgcgcacct ctgccgttac ctccccggcg aatcgttcgt caccatggtg
     3361 ttgtgcgctt tggacgtgcg caccggtgag ctcgagtgct tgaaccttgg gcaccttccg
     3421 cccttgctcg tgggcgaagg cggcagcgtg cgaccgttgc aagcggccga gaacctggcg
     3481 ctcggaatga tgccgttcac gccgcgctcc gagaagtacc agatggatcg cgacgaggtg
     3541 ctgctgctct acaccgacgg cctcaccgag ctcgagaacg gacgccgtcc cggcgaggaa
     3601 cccgcgccgc ccgacacggg gctgctcgcg ggcgcgctcg gcggttgcgt tcaaatcatc
     3661 cactcgatgc agggcgcgga catcaacgcg atccgcgacc gcatgtcaca gtcggtgcgg
     3721 atccagctcg acttgacgat ggcctccgac gacgcggcgt tcatcctcgc gcgcttgcag
     3781 aaccgccaac acctcctccc ggaggcgcgc aacgtcaccg tcgcaccgcc tcccccggcg
     3841 ctgaagcctc gctgagttcg cgcgcagcgg gccgacaaga gccgagcccg cgtcggcgcg
     3901 gtgaagcgga gcgtttcgcc cggccactcg cttcgcggcg aaaaacgccg acgcttcgag
     3961 cgcggggtgc cgatggttcc gcgc
//
LOCUS       DASTKS010001342         7066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1782011, whole genome shotgun sequence.
ACCESSION   DASTKS010001342 DASTKS010000000
VERSION     DASTKS010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782011"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFQ35_28780"
     CDS             complement(<1..243)
                     /locus_tag="VFQ35_28780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934748.1"
                     /translation="MQLSRVLLLLVPVVGIAELAFDLFFATRAPALSEWVELGTHLQG
                     ARSEGDLVVVSPSWAEPLARQAFGDALMPLADVARSD"
     gene            288..872
                     /locus_tag="VFQ35_28785"
     CDS             288..872
                     /locus_tag="VFQ35_28785"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9934749.1"
                     /translation="MSFSAAFVARAREALEKLDCEAAERLVEELCSVREREGRLFVLG
                     VGGSAANASHAVNDFRKLCGIEAYAPTDNVAELTARTNDEGWESTFAAWLRTSRLGPN
                     DLILVLSVGGGDRERNVSPNLVAAMEYAQSCGAPVIAIVGRDGGTAGRSARSSVIVPT
                     VDASLVTPITEALQGVVLHLLVSHPRLRVTNTTW"
     gene            1054..2580
                     /locus_tag="VFQ35_28790"
     CDS             1054..2580
                     /locus_tag="VFQ35_28790"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9934750.1"
                     /translation="MMKSRLTACLAFGGALIQSSAFAQAAPTSAGAPPPASPPPASPA
                     EPGEALTPGPAAAVNAEPVATAEPAGPALPQPPPHADERAEHPARFELGIFGGLFFPS
                     SSHELLTRGPWQKYESVAPELGGRVSFLPLDFAGIEIEGAAMPTKVKNGDAAAFWAAR
                     AHLILQAPLKGILPFAVIGGGALGAGSNTLGSDNDPAVHFGVGAKVPLDDFLALRLDL
                     RDNLTQKHAASQGDQTHHFEATLGLTFALRPHKAAEPPPPAAPVPDTDGDGVLDDKDA
                     CPVEQGKMPNGCPYGDRDHDGVTDDRDACPDNPGKNQCGCPPVDTDGDKVIDELDKCP
                     KEPGPIEGCPDPDGDHDGVPVPDDKCPDKAETKNGYEDSDGCPDEIPEVVKKFTGVIQ
                     GIEFDRGKETIRPVSTPILDSALKVLRDYPKVRVEISGHTDSDGTREKNVDLSKRRAE
                     SVKTYFVSRGIDESRIETRGVGPDEPIADNKTAAGKQKNRRIEFKLIDEAKRPEKGQP
                     "
     gene            2577..3464
                     /locus_tag="VFQ35_28795"
     CDS             2577..3464
                     /locus_tag="VFQ35_28795"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET9934751.1"
                     /translation="MNRSCIALASGLALLSAAPAFAQEDLPKGLMATKSASTGTENVS
                     TSGFEAPVAKAPEDDKDTTELKINAGGLVAGGNARSLAATASGKLRVRRGMNQLSAAA
                     AANYGEAAPKGEGDMVTSVENYQAKIREDRFLADHFAVFIAMSGLRDRFLGLRLRLNF
                     DPGLAYYFVDEPKHRLWLEAGYDLQHDFRRGEALAAAYAKGQLLDRSEVRHSGRLFLG
                     YENALSENFTFDTGLEYLQAFKDTENYRINWDLGITSRIGGNFSVATTLNLRYDHNPL
                     PGVKTTDVTSALSLVYQVL"
     gene            3481..5745
                     /locus_tag="VFQ35_28800"
     CDS             3481..5745
                     /locus_tag="VFQ35_28800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9934752.1"
                     /translation="MVSSPPASMLWKFRPPAPRRLFAQLARFDDGAGLPEANRDDVRL
                     GRALAALASVWFALVAFWEIGAPFGAGHYAASAAVGMAGENMLRFRTLLPVIDVPLGA
                     PTVADCYCHHPFGVFWTAALFRAFLGHHDFVCRLPAALLSAATPPLLYALGRSLWSPL
                     AGGVTALAFTLTPIALSFANFNALEVPTLFGVCLASLAYVRFLQSGRRRFVALCLAAM
                     LYAANSDFPGLVFSAELLLLVFFRVFVRGRGLDVDRFRRGAHLFAWGVLLLGALCVGY
                     VAVFAHIGKLDELLQQGRFRATGNDRPLAEVLASRRYWNLLMFTPLAIVVGKLGAVVS
                     LARAGVRRSELELLPTALLGMALFQYLVFSQGADVHVFWPHYFAVCFALGLGATAQSA
                     ADVLRLGLPKRPRAALGAYVLAALVPLFMARDALATLVYARKTGGRFGEKGAFIQPDK
                     DKVAALEYLSERAAPGDVVTLNLNMRKSLWVPWSLEHATKIDGASQFRGDERFYVTDG
                     RYSEGTMLRERSQHSGTTVVGPFWIFDRRSREPLEAFSVVKREPNLLERYFVSSTHAL
                     RRLEVDPYARWEARATIYQSPNPLPTTEPQSFEQLRIAHNIAVEQAHFERAAQLKKRL
                     LEGTDPRVATLYEDGTKLLAARFEHGASDVLTLYFESIGPDDQRFAIRSRVEAPMAGS
                     LVPADPLVREVGLPTTIPRNTWRAGFIYSSVTEILKRPGRERFYGLWLNTTSATLPLK
                     TKRGESEVTLLVLP"
     gene            5756..>7066
                     /locus_tag="VFQ35_28805"
     CDS             5756..>7066
                     /locus_tag="VFQ35_28805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9934753.1"
                     /translation="MKLSPRAVAALLAGVPVALACSTLALRRFGARLGSWPRLALENG
                     LVLAFFALALAFQYLVLALVPLDPWAPFPESAVHAAALAALAAALCPARFRGRALGAL
                     LILFVALSFGDVVYLRYFGNVLPLFAFRSGTQLWDVRDLVVDYTRGGDAWFLPSSLAG
                     LLLLAVWRTRRAFTGRLRLVELGVQLVVVGSLTFAAWPVRSDVLNWLASERSYRVLNG
                     TDAVQDSGYLIAHLKETSRAFRSLHELTHLSPSARAAVQRFHEQRAVEPPRDADFGIA
                     RGTNLLVVQIEGMQKWVIGARHAGHELTPFLNRLRERALYFDHLFDETGDSSTSDAEY
                     MLFNSQLPLSVGSVAFRRADNHFVTLLHTFKDAGYTTFAGHAYAAGMWNRAVLYPRYG
                     FDHVAFQDEFGSEPKLGWGLGDKPFVERAVPMLQRLKQPFLGFLV"
BASE COUNT         1140 a         2423 c         2268 g         1235 t
ORIGIN      
        1 gtcggagcgc gccacgtccg cgagcggcat cagcgcgtcc ccaaatgctt ggcgggcgag
       61 aggctctgcc cagctcggcg agacgacgac caaatcgccc tcgctgcgcg caccttgcaa
      121 atgcgtccca agctcgaccc attcactcag cgccggggcg cgcgtcgcga agaacaagtc
      181 gaacgcgagc tctgcgatgc cgaccacggg caccagcagc aagagcactc gcgaaagctg
      241 catcggtccg gccgaggttg ccacaaaagt ggtagcctcg cggcatcatg agtttttccg
      301 cggcattcgt ggcccgcgcg cgcgaggcgc tcgagaagct cgactgtgag gcggcagagc
      361 gactcgtgga ggagctctgc tcggtgcgcg aacgcgaggg acgcctgttc gtactcggcg
      421 tcggcggcag cgccgcgaac gcttcgcacg cggtgaacga cttccgtaag ctctgtggca
      481 tcgaggctta tgcgcccacc gacaacgtgg cggagctcac cgcgcggacc aacgacgaag
      541 gttgggagag caccttcgcg gcgtggttgc gtacgagccg gctcgggcca aacgacctca
      601 ttttggtgct gagcgtcggc ggaggagatc gcgaacgcaa cgtgagccca aacctcgtgg
      661 ccgccatgga atacgcccaa agctgcggcg ctcccgtgat cgcgatcgtt ggtcgcgacg
      721 gcggtacggc cgggcgttca gcgcgttcgt cggttatcgt ccccaccgtc gacgcttcgc
      781 tcgtgacgcc aatcacggaa gcactccaag gcgtcgtatt gcacctactc gtctcgcatc
      841 cgcgcctgcg ggtgaccaat acgacgtggt agtcgcaact tacttgacgt gagtgcagaa
      901 gagcagcgac tcttcggccc atatacctgc tcgggcagcc taacccgcgc ccgacgggtg
      961 gtgcgtggcc gcctggcttc gccggggctg gctgactggt tttgcggcag cccggcttcg
     1021 ccggactcgg ccgacatcaa ggtggacggt gagatgatga agtctcgact aacggcttgt
     1081 ctcgcattcg gcggcgcgct cattcagagc tccgctttcg cacaggcggc gccgacgtcg
     1141 gccggagccc ctccgcctgc aagcccgccg ccagcgtcgc ctgcagagcc gggggaagcg
     1201 ttgacgccgg ggcctgcagc agcagtcaac gcggagcccg tggcgacggc cgagccagcc
     1261 gggcctgcac tgccgcaacc accgccacat gcggacgagc gcgcagagca cccggcgcgt
     1321 ttcgagctcg gcatttttgg cggcttgttc ttcccgtcga gctcccacga gcttttgacg
     1381 cgcggccctt ggcaaaagta cgaatcggtc gcgcccgagc tcggcggccg cgtcagcttc
     1441 ttgccattgg acttcgccgg catcgagatc gaaggcgccg cgatgcccac caaggtcaag
     1501 aacggggacg ccgccgcgtt ctgggccgcg cgcgcacacc tcatcctgca agcgccgctg
     1561 aagggcattc tgccgttcgc tgtcatcggc ggcggcgcgc tcggtgctgg gagcaacaca
     1621 ctcgggtcgg acaacgaccc ggcggtgcac ttcggcgtcg gtgcgaaagt accgctcgac
     1681 gacttcctcg cgttgcgcct cgacctacgt gacaacttga cccagaagca cgctgcttcg
     1741 cagggagacc agactcacca cttcgaagcg acgctcggcc tcaccttcgc gctgcgtccg
     1801 cacaaagcgg ccgagccacc tccccccgcg gcgcccgtac ccgacaccga cggcgacggc
     1861 gttctcgacg acaaggacgc ctgccccgtc gagcagggca aaatgcccaa cggttgcccc
     1921 tacggcgacc gcgaccacga cggggtcacg gacgacagag acgcttgccc ggataacccc
     1981 ggaaagaatc aatgcggctg cccgcccgtc gacactgacg gtgacaaggt catcgacgag
     2041 ctcgacaagt gcccgaagga gccgggccca atcgagggtt gccccgatcc cgacggcgac
     2101 cacgacggcg tgccggtgcc cgacgacaag tgcccggaca aggccgagac gaagaacggc
     2161 tacgaagact ccgacggctg ccccgacgaa atccccgagg tcgtcaagaa gttcaccggc
     2221 gtcattcagg gcatcgagtt cgaccgtggc aaggagacga ttcgaccagt ctctacgccc
     2281 atcctcgact cggcgctgaa ggtgctgaga gactacccga aggtccgcgt cgagatcagc
     2341 ggtcacaccg acagcgacgg cacgcgcgag aagaacgtcg atttgtcgaa gcgccgcgcc
     2401 gagtcggtga agacgtactt cgtcagccgc ggcatcgacg agagccgtat cgagacgcgc
     2461 ggcgtcgggc ccgacgagcc catcgccgac aacaagaccg ccgccggcaa gcagaagaac
     2521 cgacgtatcg aattcaagct catcgacgag gccaagcggc ccgagaaagg tcagccgtga
     2581 accgctcctg catcgccctc gcctcggggc tcgcgctgct cagcgcggct ccggccttcg
     2641 cccaggaaga cttgcccaaa ggtttgatgg cgaccaagtc ggcttcaacc gggaccgaaa
     2701 acgtgtccac ctcgggcttc gaagcgccag tcgccaaggc gcccgaagac gacaaggata
     2761 cgaccgagct gaagatcaac gcgggtgggc tcgtcgccgg cggcaacgcg cgctcgctcg
     2821 cggccaccgc ttcgggaaag ctccgcgtgc gccgtggcat gaaccagctc tcggcggccg
     2881 ccgccgccaa ctacggcgaa gccgcgccca agggcgaagg cgacatggtc acctcggtgg
     2941 agaactacca ggcgaaaatc cgcgaagacc gcttcctcgc agatcacttc gcggtgttca
     3001 tcgcgatgtc cggtctgcgc gaccggttcc tcggcttgcg gctccgtctg aacttcgatc
     3061 ccggcctcgc gtactacttc gtcgacgagc ccaagcatcg gctctggctc gaagccggct
     3121 acgacttgca gcacgacttc cggcggggtg aagcgctcgc ggcagcgtac gcaaagggac
     3181 aactgctcga ccggtccgag gtccgtcaca gcggccgctt gttcctcggc tacgagaacg
     3241 cgctctccga gaacttcacc ttcgataccg ggctcgagta cctgcaggcg ttcaaagaca
     3301 ccgagaacta ccgcatcaac tgggatctcg gcatcacgtc gcgtatcggc ggaaacttct
     3361 cggtcgcgac gacgttgaac ctgcgctacg accacaaccc gttgcccggg gtgaagacca
     3421 ccgacgtgac gagcgcgctc agcttggtct accaggtgct ctgaaccaac gcggcgcggc
     3481 gtggtatcgt cgccgcccgc ttcgatgctc tggaagtttc gccccccggc gccgcgtcgc
     3541 ctgttcgcgc aactcgcgcg tttcgacgac ggcgccggct tgccagaggc gaaccgcgac
     3601 gacgtccggc tcgggcgcgc gctcgccgct ctcgcgagcg tttggttcgc gctggtggca
     3661 ttttgggaaa ttggcgcgcc gttcggcgcc ggccactatg ccgcttccgc agcggtgggg
     3721 atggccggcg aaaacatgct gcgctttcgc acgctgctgc cggtcatcga cgtgccgctc
     3781 ggcgctccga ccgtcgccga ttgctattgc caccatcctt tcggagtctt ctggaccgca
     3841 gcgctctttc gagcattcct cggccatcac gacttcgtct gccgtttgcc ggcggcattg
     3901 ctcagcgcgg cgacgccacc gctcctctac gcgctcgggc gtagcctgtg gagcccgctc
     3961 gccggcggcg tgaccgcgct cgcgttcacg ctgacgccca tcgcgctttc gttcgcgaac
     4021 ttcaatgccc tcgaggtgcc gacgttgttc ggcgtctgcc tggcgtcgct cgcctatgtg
     4081 cgctttctgc aaagtgggcg gcggcgcttc gtggcgctgt gccttgcggc gatgctctac
     4141 gccgcaaact ccgacttccc cgggctcgtg ttctcggcag agctcttgct cctcgtattc
     4201 ttccgcgtgt tcgtgcgagg ccgggggctc gatgtcgatc gctttcgtcg cggcgcgcac
     4261 cttttcgcct ggggggtttt gctgctcggg gcgctatgcg tcgggtacgt cgccgtcttc
     4321 gctcacatcg gcaaactgga cgagctcctt cagcaagggc ggtttcgcgc gacgggcaac
     4381 gatcggccgc tcgccgaggt gctcgcctcg cggcgctatt ggaacctgtt gatgttcacc
     4441 ccgctcgcca tcgtcgtcgg caagctggga gcggtcgtgt cgctcgcgcg ggcgggagtg
     4501 cggcgcagcg agctcgagct cttgcccacc gctctgctcg ggatggcgct gttccagtac
     4561 ctcgtctttt cgcaaggcgc ggacgtgcac gtcttttggc cgcactactt cgcggtctgc
     4621 tttgcgctcg ggctgggggc cacggcgcaa tcggccgcgg acgtgcttcg cctcggtctt
     4681 cccaagcggc cgcgggcggc tctcggcgcc tacgtgctgg cggctctggt gccgctcttc
     4741 atggcgcgcg atgcgctcgc gaccctcgtc tacgcgcgca agacgggcgg tcgcttcgga
     4801 gaaaaaggcg ccttcattca gccggacaaa gacaaggtcg cggcgctcga gtacttgagc
     4861 gagcgagcgg cgccgggcga cgtcgtcacg ctgaacctca acatgcgcaa gtcgctgtgg
     4921 gtgccgtggt cgctcgagca cgcgaccaag atcgacggag catcgcagtt tcggggcgac
     4981 gagcgctttt acgtcaccga cggacgctac tccgagggga cgatgctgcg tgaacgcagc
     5041 caacactcgg gcaccaccgt ggtcggcccg ttttggatct tcgatcggcg cagccgagag
     5101 ccgctcgaag cgttcagcgt cgtcaagcgg gagcccaatt tgctcgagcg ctacttcgtg
     5161 tcctcgacgc acgccctccg tcgcctcgag gtggatccgt acgcgcggtg ggaggcccgc
     5221 gcgacgattt accagtcgcc gaacccactg cctaccaccg agccgcagag cttcgagcag
     5281 cttcgcatcg cgcacaacat cgcggtcgag caggcgcatt tcgagcgcgc cgcccaactc
     5341 aaaaaacgct tgttagaggg caccgaccca cgcgtcgcga ctctgtacga ggatggcacg
     5401 aagctactcg cggcgcgctt cgagcacggt gcgtccgacg tgctcacgct ctacttcgag
     5461 tcgataggtc cggacgacca gcgcttcgcg attcgttcgc gcgtcgaggc gccgatggct
     5521 ggctcgctcg tgccggctga cccgctcgtt cgcgaggtgg ggttgccgac gaccattccg
     5581 cgcaacacct ggcgcgctgg gttcatctac agctccgtga ccgaaatctt gaagcgccca
     5641 ggacgggagc gcttctacgg gctctggctg aacaccacga gcgcgacgtt gccgctcaag
     5701 accaagcgcg gcgagagcga agtcacgctg ctcgtgttgc catgaagccc cgcccatgaa
     5761 gctctcgcca agagcggtgg cggcgctgct cgcgggtgtt ccggtcgcgc tcgcctgctc
     5821 gacgttggcg ctccgtcgct ttggcgcgcg cctcggatcc tggccgcggc tcgctctcga
     5881 aaacggcctc gtactcgcgt ttttcgcgct ggcgctcgca tttcaatatc tggtgctcgc
     5941 gctcgtgccg ctcgacccgt gggccccatt tcccgagagc gcagtgcacg cggccgcgct
     6001 cgccgcgctc gccgccgcac tctgtccggc acgctttcgc ggaagagccc tcggtgcgct
     6061 cttgatcctg ttcgtcgcgc tgtcgttcgg cgacgtagtt tacctgcggt acttcgggaa
     6121 cgtgctcccg ctgttcgcgt tcaggagcgg cacgcagctc tgggacgtgc gcgatctcgt
     6181 cgtcgactac acgcgggggg gcgacgcttg gttcctaccg agttcgctcg cagggctgct
     6241 cctgctcgcg gtgtggcgga cgcgccgagc cttcaccggg cgcctccgcc tcgtcgagct
     6301 cggggtccaa ctcgtcgtcg tcggctcgct cacgttcgcg gcatggccgg tgcgaagcga
     6361 cgtcttgaat tggcttgcca gcgagcgctc gtatcgagtg ctgaacggca ccgacgcggt
     6421 gcaagactcc ggctacctga tcgcgcattt gaaggagact tcgcgggctt ttcggagcct
     6481 gcacgagctc acgcacttgt ctcccagcgc gcgcgccgcg gtgcagcgct ttcacgagca
     6541 gcgcgccgtc gaaccgccgc gcgacgcaga cttcgggatc gcacgcggca cgaaccttct
     6601 cgtggttcaa atcgagggca tgcaaaagtg ggtaatcggc gcgcgccacg ccggacacga
     6661 gctcacccca tttttgaatc gactgcgcga gcgcgcgctc tacttcgacc acctgttcga
     6721 cgaaaccggc gactcttcga cgtcggacgc cgagtacatg ctcttcaact cgcagctgcc
     6781 gttgagcgtc ggctcggtcg cgtttcggcg cgcggacaac cacttcgtca cgttgctgca
     6841 cacgttcaaa gatgccggct acaccacctt cgcgggtcac gcctacgccg ctggcatgtg
     6901 gaaccgcgcg gtgctctacc ctcgctacgg cttcgatcac gtcgcgtttc aagacgagtt
     6961 cggctccgaa ccgaagctcg gttgggggct cggtgacaag ccgttcgtcg agcgcgcggt
     7021 gccgatgctg cagcgcttga agcagccttt tctcggcttc ctggtg
//
LOCUS       DASTKS010001343         5488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_1560219, whole genome shotgun sequence.
ACCESSION   DASTKS010001343 DASTKS010000000
VERSION     DASTKS010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560219"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFQ35_28810"
     CDS             complement(<1..220)
                     /locus_tag="VFQ35_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HET9934754.1"
                     /translation="MSADLSGKQRRYLRSLGHELSPVVQIGHQGLTEAVLSAIDQALE
                     THELIKVRALVDTKDEALALVPDVEAKLK"
     gene            340..1500
                     /locus_tag="VFQ35_28815"
     CDS             340..1500
                     /locus_tag="VFQ35_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HET9934755.1"
                     /translation="MKILYGVVGEGMGHATRSRVVIEHLATKGHEVKIVVSGRAHRFL
                     TERLADYPNVSVEQIEGLTLTYLGNALDRSASIFHNLKNAPQAVAHNVDVYRKVAEAG
                     FRPELVISDFESWAALYARRHGLPVVSIDNMQIINRCRHKKSLLKGKGFDFRVARLAV
                     KSKLPGACHYLVTTFFTPEVRKSYTSLVPPILRPEILRAKREPGEHVLVYQTQTTQHE
                     LVPTLRKLPYRFRVYGFGRDAVEGNVTLCSFSETRFVDDLRTARAAIAGGGFSLMSEA
                     VALGVPLYSIPVKGQFEQELNARYLAELEYGAYSQALEAEAVARFLDRASIYEKKLAA
                     RPRLDNSMLFGCVDELVQRVARGESRVRMLSSPALAKSDDEEEAEDEADAEG"
     gene            1575..2144
                     /gene="ruvC"
                     /locus_tag="VFQ35_28820"
     CDS             1575..2144
                     /gene="ruvC"
                     /locus_tag="VFQ35_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9934756.1"
                     /translation="MLVLGIDPGTLHLGWGVVERDGNRLRHVAHGVLNMPQGRSLSGR
                     LLDISAGLAQVIEAQKPSVASVESLFFHKDAQSAAKLGHARGVVLLCLEQAGVKLAEY
                     APARVKRTVVGHGRADKKQVAAMVRAMLGLPKAPPSDAADALALAITHLRLGALITSL
                     PQRADSQAGGPETPRERLLRLVQGGRRRR"
     gene            2244..3002
                     /locus_tag="VFQ35_28825"
     CDS             2244..3002
                     /locus_tag="VFQ35_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET9934757.1"
                     /translation="MHRFASVRSTLTLALGLTLTALAAAEPPKQAPPAKAHPAAPAVP
                     AAPAAPAEPVAAPGGLSAEEIADRVQAFYDKTKTFRAGFRQRYTVPAHNKTKDSTGSV
                     VFEKPGKMSWRYTNNGNRVVSDGKIIRIYEKENNQMYEQPLDKSQYPAALSFLLGSGS
                     LRQSFKLTKLDAKQMNCEGCYVLLGEPKEASATYQKVFLYVDAATYQVRRVMLIDAQR
                     NRNRFDFVTPEVNAKPAPGEFVFTPPAGTQVIRQ"
     gene            complement(2996..3907)
                     /locus_tag="VFQ35_28830"
     CDS             complement(2996..3907)
                     /locus_tag="VFQ35_28830"
                     /inference="COORDINATES: protein motif:HMM:NF025602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9934758.1"
                     /translation="MTLVLFPSAPMNLREIDEDFEVERQAAKKAGLATALIDHTRLVM
                     GEPLAALAGVGDYQGEVIYRGWMLTPSQYETLYTTLAGRGIHLLNDPAAYRTCHYLPD
                     SYGYIAEHTARSVWTPLSGEPNFEALANLLASFGSKPLIVKDYVKSQKHYWDEACFIS
                     QADDRVSVERVVRRFLELQADDLNEGLVFREFLPLKRIGTHPQSGMPLSAEFRIFWLD
                     AEPLLSHRYWAELGPFDSPLPIEDLRAIAEQIPSRFFSMDVAFLESGEITIVELGDGQ
                     VAGLPSPNLAPEFFRRIAHRVVDAQAH"
     gene            4064..>5488
                     /locus_tag="VFQ35_28835"
     CDS             4064..>5488
                     /locus_tag="VFQ35_28835"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9934759.1"
                     /translation="MADAVYVTGLGSFFPGAPITNDQMEEVLGRVHGHPSRARSRVLA
                     QNGIVGRHYALDREGRSTHRASEMAALALRAAFESSLHSPSSLEFLAAATTQGDVNVP
                     GFASMVHAELDSPACEIASLSGVCASGMMALKSAYLQVLSGEKRLAGACASEFPSRFF
                     KASRYEAHVPAGASLPFDVEFLRWMLSDGAGAALLAPKPAERGLSLRLEWLELTSFAH
                     LNPVCMYAGAKKRSDGELGPGWLDYPSFEAAAAEGAMYLRQDVRLLERIVELGVDGFF
                     GLVQRGKVDPALIDHVVCHYSSQAFKPRVFELLQKAGVVVPEPRWFTNLPTRGNVGSA
                     SVFVLLDELVRSGRVRPGERVFAMIPESGRFVVSYALFTAVAAERPLEVRAPKSPQAI
                     PALASAPKSAVAQSLVRELTRVWVDFEARLSAVPIVQKLETMRFTLDDYKLLLLNLRP
                     QVVEGSRWIARAASRFDAERSPLRS"
BASE COUNT          962 a         1694 c         1841 g          991 t
ORIGIN      
        1 ccttgagctt ggcttcgacg tcgggaacga gcgcaagcgc ttcgtccttc gtgtcgacga
       61 gggcgcgaac cttgatgagc tcgtgggttt cgagggcctg gtcgatggcg ctcaggacgg
      121 cctcggtgag tccttggtgg ccgatttgaa cgacggggga gagctcgtgg ccgagcgacc
      181 gcagatagcg gcgctgcttg ccggataagt ccgctgacac ggaggcgtct tacatcggtc
      241 ttcggctcga accaagagaa gtcggctcga ggactcgggg cgcgccggga gtgctcggtt
      301 ttggccgcgc gcgtggggaa tgtggcatgc tcggaacccg tgaagatcct gtacggagtg
      361 gttggcgagg gtatgggcca tgcgacgcgg agccgcgtcg ttatcgagca tttggcgacc
      421 aaggggcacg aggtgaaaat cgtggtgtcc ggcagggcac accgatttct gacggagcgc
      481 ctcgcggact acccgaacgt gagcgtcgag cagatcgaag ggctcacgct gacctatctc
      541 ggcaacgcgc tcgatcgtag tgcgagcatt tttcacaatc tgaagaatgc gccgcaagca
      601 gtggcgcaca acgtcgacgt gtaccgcaag gtggcggaag cgggctttcg ccccgagctc
      661 gtgatcagcg acttcgaatc gtgggccgcc ctgtacgcgc gccgacacgg cctgccggtg
      721 gtcagcatcg acaacatgca aatcatcaac cgctgtcgcc acaagaagtc gttgctcaag
      781 ggcaagggct tcgactttcg ggtggcgcgc cttgcggtga agtcgaagtt gcctggtgcg
      841 tgtcactacc tcgtgacgac gttcttcacg cccgaggtgc gcaagagcta cacgtcgctc
      901 gtgccgccga ttttgcggcc cgaaatcctg agagcaaagc gtgagcccgg cgagcacgtg
      961 ctcgtttatc agacgcagac gacgcagcac gagctcgtgc ccacactgcg gaagctcccg
     1021 tatcgttttc gcgtttacgg cttcgggcgc gacgccgtgg aaggcaacgt cacgctctgc
     1081 tccttctcgg agacgcgctt cgtcgacgat ttgcgcacgg cgcgcgcggc gatcgccggc
     1141 ggtggcttct ccctgatgag cgaagccgtc gcgctcgggg tcccgctcta ttcgatcccg
     1201 gtaaagggtc agttcgagca ggagctcaac gcgcgttacc tcgccgagct cgagtacgga
     1261 gcgtattcgc aagcgctcga agccgaggcc gtggcgcgat tcctcgatcg cgcgtccatc
     1321 tacgagaaga agctcgcggc tcgaccgcgg ctcgacaatt cgatgctgtt cggttgcgtc
     1381 gacgagctcg tgcagcgagt ggcgcgcggc gaatcgcgag tgcgaatgct ttcgagcccg
     1441 gcgctcgcga agtccgacga cgaagaagag gccgaagacg aggcagacgc ggaggggtag
     1501 ggcggggtgg cttgggcgcg ctctacgcca attatcttgc ccgatacctg gtcaaacgtt
     1561 tagagaagag gggcgtgctc gtcctcggca tcgaccccgg aactcttcac ctcggttggg
     1621 gtgtggtcga gcgtgacggc aatcggctgc ggcatgtcgc gcacggcgtt ctcaatatgc
     1681 cccagggacg tagcctctcc ggccgcctgc tcgacatctc ggcgggtctc gcgcaggtga
     1741 ttgaggcgca aaagccgtcg gttgcgtcgg tcgaatcgct attttttcac aaggacgcgc
     1801 agtcggcagc gaagctcggg cacgcgcgtg gcgtcgtgct gctttgcctc gagcaagcag
     1861 gggtgaagct cgcggagtat gccccggcgc gggtcaagcg gacggtggtg gggcacgggc
     1921 gcgcggacaa gaagcaggtc gcggcgatgg tgcgcgcgat gttggggctg ccgaaagcgc
     1981 cgcccagcga cgccgccgat gcgctcgcgc tcgccatcac gcatctccgg ctcggagcgc
     2041 tgatcacatc gctgccgcag cgcgcagatt ctcaggcggg cggcccggag acaccccggg
     2101 aacggctctt gcgtctcgtt cagggcggtc ggcgccgccg ctaaacggtg ctaggctccg
     2161 ctcgcgcgga aaaaagccaa gcctatcgcg tacatggccg caagcgaaag cttcggcccc
     2221 tctcgaacgt agatctcact gccatgcacc gctttgcctc tgttcggtcg accctcactt
     2281 tggctctcgg cctcactttg acggctctcg ccgcggccga gccgcccaag caagcgccgc
     2341 ccgccaaggc gcatccggcg gctccggctg tcccggccgc tcccgctgcc cccgccgagc
     2401 cagttgcggc cccgggcgga ttgagcgccg aagagatcgc cgaccgcgtg caggctttct
     2461 acgacaagac caagaccttt cgcgcgggct ttcggcagcg ctacacggtg cccgcgcaca
     2521 acaagaccaa ggacagcacg ggctcggtcg tcttcgagaa gccgggcaag atgagctggc
     2581 gttacacgaa caacggcaac cgcgtagttt ccgacggcaa gatcatccgc atctacgaaa
     2641 aagagaacaa ccagatgtac gagcagccgc tcgacaagag ccagtacccg gcggcgctct
     2701 cgttcttgct cggcagcggc agcctgcgcc aaagcttcaa gctcaccaag ctcgacgcga
     2761 agcagatgaa ctgcgaaggc tgctacgtgc tcctcggtga gccgaaagag gcgagcgcga
     2821 cgtaccagaa ggtgtttctg tacgtcgatg cggctaccta ccaagtacgg cgcgtgatgc
     2881 tgatcgatgc tcagcggaac cgtaaccgct tcgacttcgt gacgcccgaa gtgaacgcga
     2941 agccggcgcc gggagagttc gtgttcactc cgccagccgg cactcaagtc attcgtcagt
     3001 gagcttgcgc gtcgacgacg cgatgagcaa tgcgccgaaa gaactccggc gccaggttcg
     3061 gtgacgggag ccccgcgact tgcccgtctc cgagctcgac gatggtgatc tcgcccgact
     3121 cgaggaaggc gacgtccatg ctgaagaagc gactcgggat ttgctctgca atcgcgcgca
     3181 gatcttcgat ggggagcggg ctatcgaaag gcccgagctc ggcccagtaa cggtgagaga
     3241 ggagcggctc ggcgtccaac cagaagatgc ggaactcggc tgacaacggc attccgcttt
     3301 gcggatgcgt tccgatgcgc ttcagcggca agaactcgcg aaagacgagc ccttcgttca
     3361 agtcgtcggc ctgcagctcg aggaagcgcc gaacgacgcg ctcgacgctc acgcggtcgt
     3421 cggcttgcga gatgaaacac gcctcgtccc agtagtgctt ctgggacttc acgtagtctt
     3481 tcacgatgag cggcttcgag ccgaagctag ccaacagatt ggcgagcgcc tcgaagttgg
     3541 gttcaccgct gagcggcgtc cacaccgagc gcgccgtgtg ctcggcgatg taaccgtagc
     3601 tgtcgggtaa gtagtgacag gtgcgatacg cggcggggtc gttgagcaaa tggatgccgc
     3661 gaccagcgag cgtcgtgtag agcgtttcgt actgcgacgg cgtgagcatc cagccgcgat
     3721 aaatgacttc cccttggtag tcgccgacgc cagctagagc cgcgagcggc tcccccatca
     3781 cgagcctcgt atgatcgatg agagccgtcg ccagaccggc tttcttggct gcctggcgct
     3841 cgacctcgaa atcttcgtcg atttcgcgca gattcatcgg cgctgacgga aacaggacga
     3901 gcgtcatctt ttcgtttcct actacgaaac gtcgcggtga cgaaatgcgg attctcgcct
     3961 cgtgaggcgc tttggcagcg tacggagagc acgctatgct gcgcgctcga gtgggagacg
     4021 agacggggca tgaacgtcat gaatagcgac ctgagcgaga gccatggctg acgcggtgta
     4081 cgttaccggg ctcggcagtt tcttcccggg ggctccgatc acgaacgacc agatggagga
     4141 ggtcctcggt cgcgtccacg ggcatccgtc gcgcgcgcgc agccgcgtgc tcgctcaaaa
     4201 tggaatcgtc gggcgacact acgcgctcga tcgcgagggg cgtagcacgc atcgggcgag
     4261 cgagatggcg gcgctcgcgt tgcgggccgc gttcgagtcg agcctgcact ccccgtcgtc
     4321 gctcgagttc ttggccgccg cgacgacgca aggggacgtc aacgtgcccg gtttcgcgag
     4381 catggtgcac gccgagctcg atagcccagc ttgcgaaatt gcttcgctct ctggcgtgtg
     4441 cgcgagcggg atgatggcgc tgaagtccgc ctacctgcag gtcctgagcg gagagaagcg
     4501 cctcgccggg gcgtgcgcga gcgaattccc cagccgcttt ttcaaggcga gtcgctacga
     4561 agcgcacgtg cccgcgggtg cgtcgctgcc gttcgacgtc gagtttttgc gctggatgct
     4621 ctcggacggc gcgggcgcgg cgctgctcgc gccgaagcct gccgagcgcg gtctttcgtt
     4681 gcgactcgag tggctcgagc tcacgtcgtt cgcgcacctg aacccggtgt gcatgtacgc
     4741 gggcgcgaag aagcggagcg acggcgagct cggtcccggt tggctcgact acccgagctt
     4801 cgaggcggcg gccgcagaag gcgcgatgta cttgcgccaa gacgtgcggc tgctcgaacg
     4861 catcgtggag ctcggcgtcg acggcttttt cggcctcgtg caacgcggta aggtcgaccc
     4921 ggccctcatc gatcacgtcg tgtgccacta ttcatcgcaa gccttcaagc cgcgtgtctt
     4981 cgagctgctg caaaaagccg gagtcgtcgt gcccgagccc aggtggttca ccaatttgcc
     5041 gactcgaggc aatgtcggtt cggcgtcggt cttcgtgctg ctcgacgagc tcgtgcgctc
     5101 ggggagggtg cggccggggg agcgcgtgtt cgcgatgatc cccgagagcg ggcgcttcgt
     5161 cgtaagttac gctctcttca ccgcagtcgc tgcggagcga cctctcgagg tgcgtgcacc
     5221 gaagtcgccg caagccattc ccgcgctcgc ttccgccccc aagagcgcgg tggcacaaag
     5281 cttggttcgt gagctcacgc gcgtttgggt tgacttcgag gcgcggctct cggctgtacc
     5341 catcgtgcag aagctcgaga cgatgcggtt cacgctcgac gactacaaat tgctgctatt
     5401 gaatctgcgg ccgcaggtgg tcgaaggttc tcgctggata gcgcgcgccg cttcgcgctt
     5461 cgacgcggag cgctcgccgc ttcggagc
//
LOCUS       DASTKS010001344         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_666646, whole genome shotgun sequence.
ACCESSION   DASTKS010001344 DASTKS010000000
VERSION     DASTKS010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666646"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..577
                     /locus_tag="VFQ35_28840"
     CDS             422..577
                     /locus_tag="VFQ35_28840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934760.1"
                     /translation="MAHALVELVPATPSAGGRGRYLLEVAGARIEFGDDVSVATLRRV
                     LEAVRSC"
     gene            571..960
                     /gene="tnpB"
                     /locus_tag="VFQ35_28845"
     CDS             571..960
                     /gene="tnpB"
                     /locus_tag="VFQ35_28845"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9934761.1"
                     /translation="MLSLPPTVRVFVAVAPLDMRGSFDALAGAVRRLGLDPVDGHVYL
                     FFKKRRRLAKGIWFDGSGWCLLAKRLEAGSFQLPPFDGAQPQVAIDGAAFASLLAGID
                     FTVARRGWYRRPRVEKVIKTIDTDLRV"
     gene            1010..>2218
                     /locus_tag="VFQ35_28850"
     CDS             1010..>2218
                     /locus_tag="VFQ35_28850"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET9934762.1"
                     /translation="MLRGSLAEQRAQIAQLIDQLARLNERVSELLAVAQRKQRKSAAP
                     IAALPPVAPPIVAEEARRAFEERPKAPEKPADKLWRKKKSSPTGRKPVPGHLEAEEHE
                     LRPDACEKCGGTALDVVDEFVEEKLHVVKEHQRRRVVRRYTCRCRACRERTTLRSLPA
                     PYERSKVTCEWLAWFVYQKFWLLTPLDHIRRDLAERGIPIAMSTLVTFIERAADLLST
                     IDGLHWKQLLASSWMATDGTGLKVIIPELPVAHNGYVELYRNDAVAVFQYEPTKDGDI
                     VAAKLKPFRGVLTADAEHRFNDVFASGDVVEAGCNAHGRRKFRDAEATQPVLAVEGGA
                     FLGAIYGEEEKAQKLRLTADALREHRQRFIRPIAQNFERWLDAVGPTLLTSEPLAEAI
                     AYYQNHRAALF"
BASE COUNT          386 a          702 c          754 g          376 t
ORIGIN      
        1 gggggcggag ccgatcgaga tcgcggtgag cgagagcgtg acgcgcgtcg tcgggtcgag
       61 cgtgttcgag gtggtggtcg gggacgcgct tgtgcgctgc gacgtgggca cggatgtgcg
      121 gtacgtggcg gagttggtgc gtgacccatc agcgacgccg ggatgatttc gagaagtgcg
      181 ggagtcaggc tcgatcgcga caaggaggtc gcgagtggca gtcggacgaa agatcgaaga
      241 cgagggcgaa gcgcagcggt gcctgtcggc agcacggcgt tcgggtttga gcgcggggga
      301 gtgagggcga gcgcacggcg tggacggacg ctcacttcat gcgtggcaga tgaatctcga
      361 gcggcggtcg tcggatggtc cccgccagaa acgtcgaaag ccgaagccaa agaggacggg
      421 ggtggcgcac gctctcgtcg agctggttcc ggcgacaccc agcgctggcg ggaggggccg
      481 atacctgctc gaggttgccg gtgctcggat cgagttcggg gacgacgtct cggtcgcgac
      541 gcttcgtcgt gtgctcgagg cggtgcgctc gtgctgagtc tcccgccgac ggtgcgggtg
      601 ttcgtggccg tcgcgccgct cgacatgcgc ggctcgtttg acgccctcgc tggcgccgtg
      661 cgacgcctcg ggctcgatcc cgtcgacggc cacgtctacc tcttcttcaa aaagcgcaga
      721 cggctcgcga aagggatttg gttcgatggc tcgggctggt gcttgctcgc gaaacgcctc
      781 gaagccggaa gtttccagct tccgccgttc gacggagccc agccgcaagt cgccatcgac
      841 ggcgcggcgt tcgcctcgct tctcgccggg atcgacttca ccgtcgcgcg gcgcggctgg
      901 taccgacggc ctcgcgtcga aaaagtgatc aagacgatcg acacggatct ccgagtgtga
      961 tctgtactcg tctgtggacg agctcgaagc gctgcgaaaa gagaatgccg tgcttcgcgg
     1021 atctctcgcg gagcagcgcg cgcagatcgc ccagctcatc gaccagctcg cgcggctcaa
     1081 cgagcgcgtg tccgagctac tcgcggtagc gcagcgcaaa caacgaaagt ccgctgcgcc
     1141 gatcgcggca ctgccgccgg tcgcgccacc gatcgtcgcg gaggaggcgc ggcgcgcctt
     1201 cgaggagcga cccaaggcgc ccgagaagcc ggccgacaaa ctctggcgga agaagaagtc
     1261 gtcgcctacc ggcagaaagc cggtccccgg ccatctcgaa gccgaagagc acgagcttcg
     1321 tcccgacgct tgcgaaaagt gcggcggcac cgccctggat gtcgtcgacg agttcgtcga
     1381 agagaagctc cacgtcgtca aagagcatca gcgaaggcgc gtcgttcgtc gctacacatg
     1441 tcgctgtcgt gcctgtcgcg agcgtacgac cctgcggtca ctgccggcgc cctacgagcg
     1501 ctcgaaggtc acctgcgagt ggctcgcttg gttcgtttac cagaagttct ggctgctcac
     1561 gccgctcgac catatccggc gcgacctggc cgagcgcggc attccgatcg cgatgagcac
     1621 tctcgtcacc ttcatcgagc gcgctgccga cttgctctcg actatcgacg ggctgcactg
     1681 gaagcagctg ctcgcaagct cgtggatggc tacagacggt accgggttga aggtcatcat
     1741 cccggagcta ccggttgcgc acaacggcta tgtcgagctc taccgaaacg acgcggtcgc
     1801 cgtcttccag tacgagccga ccaaggacgg cgacatcgtt gccgcaaagc tcaagccgtt
     1861 tcgtggcgtt ctcaccgccg acgcagagca tcgcttcaac gatgtcttcg cttcaggcga
     1921 cgtggtcgag gccggctgca acgctcacgg ccgtcgcaag tttcgcgacg ccgaagccac
     1981 tcagcccgtg ctcgcggtcg aaggcggcgc gttcctcggc gcgatctacg gtgaagagga
     2041 gaaggctcag aaactccgcc tcacggccga cgcgctccga gaacatcggc agcgattcat
     2101 tcgacccatc gcccaaaact tcgagcgctg gctcgatgcc gtcgggccca cgctcctaac
     2161 ctccgagccg ctggcggagg cgattgctta ctatcaaaat caccgcgctg cgctcttc
//
LOCUS       DASTKS010001345         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_890564, whole genome shotgun sequence.
ACCESSION   DASTKS010001345 DASTKS010000000
VERSION     DASTKS010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890564"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFQ35_28855"
     CDS             <1..193
                     /locus_tag="VFQ35_28855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934763.1"
                     /translation="FNDLLELARAYERGEWTAVAPHLDALGVDPHVVAQCFVDSLDWA
                     RQTFLAQSDTRISAAPGGR"
     gene            220..834
                     /locus_tag="VFQ35_28860"
     CDS             220..834
                     /locus_tag="VFQ35_28860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934764.1"
                     /translation="MNEAQHQNGIHGADLARVAALNEACKVLLERSFHVNLRALDAIV
                     QSKRAGAALRGFDEVAGQMRTWSRDLHHDLERLVDCSRRVVVTTSLAAKNEHSLRLLS
                     DAARISENAGAARVRDRRVDELASLDRDIGRLWREVRDLLHDLDQLGMMAAVLSRSAM
                     IEAASGSDEQRGQLGEVAREFYGNAQAVLETLQLAVKIARREKQ"
     gene            831..1574
                     /locus_tag="VFQ35_28865"
     CDS             831..1574
                     /locus_tag="VFQ35_28865"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9934765.1"
                     /translation="MKKATVIYSNNDHSWTVIARDPERPDHLVDTNEYLVRCGEQSLL
                     TDPGGQEVFPAVFSAVSATSDPRKVRSIFASHQDPDVISSLALWLAFNDDIRCYASWL
                     WCTFIPHFGGGQKTFIPVPDNGMHFELGGRELHIIPAHYLHSSGNLHLYDPQAKILFT
                     GDVGAALLPPESSDLFVKDFDSHIRHATGFHRRWMGSREARDSWCRRASELKIDMLCP
                     QHGAIYQGKDVQRFIEWFAALEIGVLRGE"
     gene            complement(1691..2113)
                     /locus_tag="VFQ35_28870"
     CDS             complement(1691..2113)
                     /locus_tag="VFQ35_28870"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9934766.1"
                     /translation="MNDEFSDELKSLVSTMSETVFGSDVCVELDSSAIPTEALWAHIA
                     IDGDGRTHVWVGPSRSLVARAAQRMLLSESVSESDLEDATREFANIIAGNLKAVFAPN
                     GAVGLPELKTPPFVPALARRSEVRLRIDGEPLSVFIDV"
     gene            complement(2110..>2482)
                     /locus_tag="VFQ35_28875"
     CDS             complement(2110..>2482)
                     /locus_tag="VFQ35_28875"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9934767.1"
                     /translation="RARAGVYTQLEVGRGLASPLLVKYFERSGIDFRIKEELRKRTTW
                     QVMNLTQLGTCFGRFDIIFLRNVLIYFSLDTRARVLASIASAMHPGSYLFLGSTETTY
                     GCSDKLEPVTLGKSTAYRLKA"
BASE COUNT          452 a          810 c          786 g          434 t
ORIGIN      
        1 gttcaacgat ttgctagagc tcgcgcgggc gtacgagcgc ggcgaatgga cggcggtcgc
       61 ccctcacctc gatgcgctcg gcgtcgatcc gcacgtcgtc gcccagtgct tcgtcgactc
      121 actcgactgg gcccgccaga cctttctcgc tcagagcgac acacgcattt ctgcggctcc
      181 tggcggtcgc tgagactcgc acacgacagg atcaacgcct tgaacgaagc gcaacaccaa
      241 aatggcatcc acggagccga cctcgcgcgc gtggccgcgc tgaacgaggc gtgcaaggtc
      301 cttctcgagc gatcgttcca cgtgaacctg cgggcgctcg acgcgatcgt acagagcaaa
      361 cgcgcggggg cggcgctgcg cggcttcgac gaagtcgcgg gtcagatgag gacctggagc
      421 cgcgatctgc atcacgacct cgaacgcctc gtagactgca gccgccgcgt cgtcgtcacg
      481 accagcctcg ccgccaagaa cgagcattcg ctacggctcc tttcggacgc cgcgcgcatc
      541 agcgagaacg ccggcgccgc gcgggttcgc gatcggcgcg tcgacgagct cgcgtctttg
      601 gatcgcgaca tcggtcggct ttggcgcgag gtgcgtgact tgctccacga cctggatcag
      661 ctgggcatga tggcggcggt gctctcgcgc tccgcgatga tcgaggccgc gagcggctcg
      721 gacgagcaac gcgggcagct cggagaggtc gcgcgcgaat tctatggcaa cgcccaggcg
      781 gtgctcgaga ccctacagct cgcggtcaag atcgcgcggc gggagaagca atgaagaagg
      841 caacggtcat ctactccaac aacgaccact cctggaccgt gatcgcgcgc gatccggagc
      901 gcccggatca tctcgtcgac acgaacgaat acctggtgcg ctgcggcgag cagtctttgc
      961 tcaccgaccc gggcgggcag gaggtgtttc cggcggtatt ttccgcggtg agcgccacct
     1021 ccgacccgcg caaggtgcgc agcatcttcg cgtctcacca agatccggac gtcatctctt
     1081 cgctcgccct gtggctcgcg ttcaacgacg acatccggtg ctacgcgagc tggctctggt
     1141 gcacgttcat cccgcacttc ggcggcggcc agaagacctt catccccgtc cccgacaacg
     1201 ggatgcactt cgagctcgga gggcgggagc ttcacatcat acccgcgcat tacctgcact
     1261 cttccggcaa cctgcacctc tacgatcccc aggcgaagat cctcttcacg ggagacgtgg
     1321 gggcggccct cttgccgccc gagtcgagcg acctattcgt gaaagacttc gactcgcaca
     1381 tccgacatgc gaccggcttt catcgccgct ggatgggctc gcgagaggcg cgcgacagtt
     1441 ggtgtcggcg cgcgtccgag ctcaaaatcg acatgctgtg cccgcagcat ggcgcgattt
     1501 atcagggcaa ggacgtgcag cgcttcatcg aatggttcgc cgccctcgaa atcggagtcc
     1561 tgaggggcga atagccgcgc ggcgctcggg gagagagcgg acagacgatg tcgagtccgt
     1621 ctcgctctca cgaagacgaa cttcgggctg agttcgggat gacgcggcag atagcgcgga
     1681 agcgctagcg ctagacgtcg atgaagacgc tgaggggctc gccgtcgatc ctgagtcgaa
     1741 cctcggagcg gcgcgccagg gcggggacga acggcggcgt tttcagctct ggcagaccga
     1801 cggcgccgtt cggcgcgaag acggccttca ggtttccggc gatgatgttc gcgaactctc
     1861 tcgtcgcgtc ctcgagatcg ctctcgctca cgctctcgct gagcaacatg cgctgcgcag
     1921 cccgggcgac cagcgagcgc gaaggcccca cccacacgtg ggtccgaccg tctccgtcga
     1981 tggcgatgtg agcccagagc gcttcggtcg ggatcgcgct cgaatcgagc tcgacgcaca
     2041 cgtctgaacc gaacacggtc tcactcatcg tgctcacgag agatttgagc tcgtccgaaa
     2101 attcgtcgtt catgctttga gcctataagc ggtggacttt ccgagcgtca ccggttcgag
     2161 cttgtccgag cagccatagg tcgtctcggt cgagccaagg aacaggtagc tacccggatg
     2221 cattgcgctc gcaatcgacg cgagcacgcg cgctcgcgta tccaaactga aatagatgag
     2281 cacgtttcgt aagaagatga tgtcgaaacg gccgaagcag gttcccagct gagtgagatt
     2341 catcacctgc caggtagttc gcttgcgtag ctcttctttg atccggaagt cgattccgct
     2401 ccgctcgaag tacttgacga gcagcgggct cgcgaggccg cggcccacct cgagctgcgt
     2461 gtacacccca gcccgcgcgc gc
//
LOCUS       DASTKS010001346         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_222096, whole genome shotgun sequence.
ACCESSION   DASTKS010001346 DASTKS010000000
VERSION     DASTKS010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222096"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFQ35_28880"
     CDS             <1..857
                     /locus_tag="VFQ35_28880"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9934768.1"
                     /translation="VTGATGKLGTAVCRALLERGATVRATDQRFVAGFPTRVELGDLR
                     DEMFVYRITEGVKAVVHLGNHPNAFVGLSPQRLLAENTAMNTNVFHAAFDLGIKTLVF
                     ASSVQVTMRRDRDNSAPPFDVPYLPLDSDLPPNPGLNTYAQSKEFAERMLRLFVKAEP
                     SLSATAIRFPMLVTDWLHQRMTAGRIPLPNIDFTECTAHLYMEDAGRLVADVLERSLP
                     GYHQYFPAETMELRGYPFARWVPERYANTELRRPLAEFERLVDNSLMERELGWRPTRR
                     LSVEIEQR"
     gene            953..1885
                     /locus_tag="VFQ35_28885"
     CDS             953..1885
                     /locus_tag="VFQ35_28885"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9934769.1"
                     /translation="MDVLTDVLQTVRARGACSGRIEAASPWGIELPAADAARFHIILE
                     GRLWLRCDGAEPIELRRGDLVALPHGDGHVLFDSEDSPRRPLADIIAERKEKGVLSVG
                     GDGPRATFWSGRIEFEDWRGNPLLSVLPRVILLRGELARSVQWLEPTLAILSCESRSD
                     RPGAQTVVSRLADVIFIQIVRGHLATLGRDGTGWLAALGDPQVGGALALVHQNPERDW
                     TVQELAQRVAMSRSAFAARFTRMVGEPPLHYVTRWRMQKAAALLREGQSSIAQIAESV
                     GYESEAAFSKAFKRALGTSPGAYRRAARPMLDAA"
     gene            complement(1960..2922)
                     /locus_tag="VFQ35_28890"
     CDS             complement(1960..2922)
                     /locus_tag="VFQ35_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9934770.1"
                     /translation="MVALEVNEIQAARERLAAVRAEVGRVYIGDARAVETMLVALLAR
                     GHVLLEGVPGIAKTTLVKAFAVALGCSVRRIQFTPDLLPADITGTYVLNPKDGTFALR
                     PGPIFANVLLADEINRAPAKTQSALLEAMQEHQATIEGDRFALPDPFFVLATQNPVDL
                     EGTYPLPEAQIDRFLVRVQMDYPNQRDEVAMLRAHGVAAPEAKAVLAPGEVLSLQSVA
                     ARVHVEEDLLEYAVQLARYTRESPHVTLGASPRASLSLLRAGKAHALLDGRTFVTPDD
                     IRAAAVPVLAHRLVLVPELEADARARARLVEEGLARVSYRRAAR"
     gene            complement(2939..>3496)
                     /locus_tag="VFQ35_28895"
     CDS             complement(2939..>3496)
                     /locus_tag="VFQ35_28895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934771.1"
                     /translation="LSQAVSSASDALAAMHQDGLPSPIALALAALTAVAALGWSGVHA
                     MRRYRQTQPRHALPTPLLAQGGVPGRAAVLAAPTTDRALVVMELDGLLAEELALRAGL
                     PSGTPAEESLTVLERAGMPIREIRATRSALALGQRARDCVLRGRPSRIGAGQLAAHER
                     LINEVLATLERASGEGNLGISSGGR"
BASE COUNT          569 a         1154 c         1180 g          593 t
ORIGIN      
        1 tggtgaccgg cgccaccggc aagctcggca cggccgtgtg tcgtgctttg ttggaacgtg
       61 gcgcaaccgt tcgcgcgacg gatcagcgct tcgtcgcggg cttccccaca cgcgtcgagc
      121 tcggcgactt gcgagacgag atgttcgtct accgcatcac cgaaggggtc aaggcggtag
      181 tgcatctcgg aaaccacccg aacgcgttcg tcggcctctc gccgcagcgg ctgctcgccg
      241 agaacacggc catgaacacg aacgtcttcc acgctgcgtt cgatctcggc atcaagacgc
      301 tggtgttcgc gagctcggtg caagtaacga tgcggcgcga tcgcgacaat tcggctccac
      361 ccttcgatgt tccgtatctg ccgctcgact ccgacctgcc gccgaacccc ggcctcaaca
      421 cgtacgcgca gagcaaggaa ttcgccgagc ggatgctgcg cctgttcgtg aaagcggagc
      481 cgtcgctcag cgctaccgcg attcgatttc ccatgttggt cacggactgg cttcaccaac
      541 gcatgacggc tgggcgcatc ccgctgccca acatcgactt caccgagtgc accgcgcatc
      601 tctacatgga ggacgcgggt cgtctcgttg ccgacgtgct ggagcggagc ctacccggct
      661 atcaccagta ctttccggcc gagacgatgg agcttcgggg ctacccgttc gcgcgatggg
      721 tccctgagcg ctacgcgaat acagagctga gacgtccgct cgccgagttc gagcgcctgg
      781 tcgacaattc actcatggag cgagagctcg gctggcgccc tacccgcaga ctctcggtgg
      841 aaatcgagca gcgctgaaat tcgctcctga ggcgcaatgg ccggcgggcc tattttcaca
      901 taacgaacgc aaccagaaac ccacgtcggg gcgctttcgt aaccggcgct gcatggatgt
      961 cctgaccgac gttctgcaga cagtgcgcgc tcgcggcgcc tgctccggtc gcatcgaagc
     1021 ggcgtcgcct tggggcatcg agctccccgc ggctgacgct gcgcgattcc acatcattct
     1081 cgaaggtcgg ctctggcttc gttgtgacgg cgccgaacca atcgaacttc gccgcggtga
     1141 cttggtggca ctcccacacg gggacggtca cgtcctcttc gattcggagg acagcccgcg
     1201 ccggccgctg gctgacatca tcgccgaacg caaggaaaag ggcgtcctat cggttggcgg
     1261 ggacgggccg cgcgccacgt tctggagcgg gcgcatcgag ttcgaagatt ggcggggcaa
     1321 cccgctgctc tcggtcttgc cgcgcgtcat cttgctgcgc ggtgagctcg cgcgctccgt
     1381 tcagtggctc gagccgacgc tcgcgatcct gtcatgtgag agccgaagcg atcggcccgg
     1441 ggcgcagacc gtggtcagcc ggctcgcgga cgtgatcttc atccagatcg tacggggtca
     1501 tctcgccacg ctcggacgag acgggacggg ctggctcgca gcgctcggag atcctcaggt
     1561 gggtggcgcg ctcgcgttgg tccaccaaaa tccagagcgc gattggaccg tgcaggagct
     1621 cgcgcagcgt gtggcgatgt cgcgttcggc gtttgctgcg cgcttcactc gcatggtggg
     1681 cgagcctcct ctccactacg tgacgcgttg gcgcatgcag aaggccgcgg cgctgttgcg
     1741 cgaggggcag tcgagcatcg cgcaaatagc cgagtccgtt gggtacgagt ccgaagctgc
     1801 gttcagcaag gctttcaaac gcgcgctcgg cacgtcgccg ggagcatacc gccgcgcagc
     1861 tcgcccgatg ctcgacgctg catagccgct cgcgctacta ccgaaccaac gcggccaaac
     1921 gcaggtggtc ggagcttcgc gtgcgagagg caattggtgt cagcgagcgg cgcgacggta
     1981 gctgacgcgc gcgagtcctt cttcgacgag tcgcgcccgc gcgcgtgcgt ctgcttcgag
     2041 ctcgggcacc agcacgaggc gatgagccag caccgggacg gccgccgcgc ggatatcgtc
     2101 tggcgtgacg aacgtgcgcc cgtcgagcaa agcgtgcgct ttgccggccc ggagtaggct
     2161 gagcgaggct cggggagacg cgcccaacgt cacgtggggg ctctcgcgcg tgtagcgggc
     2221 gagctgtacc gcgtattcga gcaggtcctc ttcgacgtgc acgcgcgcgg cgacggactg
     2281 gagcgaaaga acctcgcccg gcgcgagcac cgccttggct tcgggtgccg ccacgccgtg
     2341 agcgcgcagc atggccacct cgtcgcgttg gttcggataa tccatctgta cgcgcacgag
     2401 gaaccgatcg atctgcgctt cgggcagggg ataagtaccc tcgagatcga ctgggttctg
     2461 cgtcgccaag acgaagaacg gatcgggcag cgcgaagcgg tcgccttcga tcgtcgcttg
     2521 gtgttcttgc atcgcctcga gcagggcaga ctgagtcttc gccggcgcgc gattgatctc
     2581 gtccgcgagc agtacgttcg cgaagatcgg tcccggtcgc agtgcaaagg tgccgtcctt
     2641 cgggttcaag acgtacgtgc cggtgatgtc cgcgggcagc agatccgggg tgaactggat
     2701 tcggcgcacc gagcagccga gcgccaccgc aaaagccttg accagggtcg tcttcgcgat
     2761 gccggggacg ccctcgagca gcacgtgccc tcgcgcgagg agcgcgacca acatcgtctc
     2821 cacggcccgt gcatcgccga tgtagacgcg tcccacctcg gcgcgtacgg ctgccaagcg
     2881 ttcgcgcgcc gcctgaatct cgttcacctc gagggccacc atgggaaact ccctcgtact
     2941 accttccccc gctcgagatc cctagatttc cctcgccgct cgctcgctcc aacgtcgcga
     3001 gcacctcgtt gatgaggcgc tcatgagcgg ccagttggcc ggcgccaatg cggctcggtc
     3061 gaccgcgcag cacgcaatcg cgggcgcgct gtccgagcgc gagagcgctt cgcgtcgcgc
     3121 ggatctcgcg aatcggcatg cctgcgcgct cgagcacggt gagcgactcc tccgccggag
     3181 tgccggacgg aaggcccgcg cgtagggcca gctcttcggc gagtagccca tcgagctcca
     3241 tgacgacgag cgcgcgatcc gtggtgggtg cggcgagcac cgcagcgcga cccggaaccc
     3301 ctccctgtgc gagtagcggc gtggggagcg cgtggcgcgg ttgagtctgt cgataacgcc
     3361 gcatggcgtg cacaccggac cacccgagcg cggcgaccgc ggtgagcgcg gcgagagcga
     3421 gcgcgatcgg cgagggcagg ccgtcctggt gcatcgcggc gagagcgtcg ctggcgctgg
     3481 agacggcttg ggatag
//
LOCUS       DASTKS010001347        11948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_890656, whole genome shotgun sequence.
ACCESSION   DASTKS010001347 DASTKS010000000
VERSION     DASTKS010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890656"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..165)
                     /locus_tag="VFQ35_28900"
     tRNA            complement(89..165)
                     /locus_tag="VFQ35_28900"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Met,seq:cat)
     gene            418..1023
                     /locus_tag="VFQ35_28905"
     CDS             418..1023
                     /locus_tag="VFQ35_28905"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9934772.1"
                     /translation="MSTREKPLRLAVFPPEVLGGEGACTGASASAAASLTGEATGSSN
                     PDSWLQVGELAKATGKTVRAIHLYEELGLLRTHVRSKGRYRLFTEEAVVRVRWIVKMQ
                     SLGLSLTEIQELVREQEASGSAQFAASRLREVYRTKLAETRNKMSELRALEAELEESL
                     AYLDACDTACQSTLPVHSCPSCERHPERPNAPDLVAGVHVN"
     gene            1134..2357
                     /locus_tag="VFQ35_28910"
     CDS             1134..2357
                     /locus_tag="VFQ35_28910"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HET9934773.1"
                     /translation="MALKFPIYMDNHATTPMDPRVLEAMLPFFTQEFGNAASRTHAYG
                     WHAEAAVDSARETVANFINAESGKEIVFTSGATESDNLAIKGVAEYYGGKGKHIVTTT
                     IEHKAVLDSCKRLEKQGFEVTYVSPGQDGLVDPADIQKALTEKTILVSVMLANNEVGT
                     IQPIEEIGAITRARGVLFHVDAVQGVGKTPFDVRAMNVDLASLTAHKIYGPKGIGALY
                     VRRSKPRVRLVAQMDGGGHERGNRSGTLNVPGIVGFGKAVEILRQEGEAEALRLRGLR
                     DRLWKRLTSELDQVILNGSAERRLPGNLNCSFAFVEGEGLMMAIKDVAVSSGSACTSA
                     SLEPSYVLRSMGLDEDLAHSSIRFGIGRFNTEEEVDYVGDLVASRVKKLRDMSPLWEM
                     HQAGIDIKSIEWVAH"
     gene            2448..2858
                     /gene="iscU"
                     /locus_tag="VFQ35_28915"
     CDS             2448..2858
                     /gene="iscU"
                     /locus_tag="VFQ35_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HET9934774.1"
                     /translation="MAYSEKVIEHYENPRNVGALDKDDPSVGTGLVGAPACGDVMRLQ
                     IRVNDAGVIEDAKFKTFGCGSAIASSSLATEWIKGKSIEEAAGVKNSQIAEELNLPPV
                     KIHCSVLAEDAIKSAIEDYKKKRAAREALESGRS"
     gene            2984..3355
                     /locus_tag="VFQ35_28920"
     CDS             2984..3355
                     /locus_tag="VFQ35_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9934775.1"
                     /translation="MSPRSEAPVPSSKRTIFVSDQAVVFAKQKLEQRGTPHASVRLGI
                     RGGGCSGFSYVIQFEDEAPRERDNVFEYGGVRFVIDKKSLIYLAGSTLDYEKTLMFQG
                     FKFRNPNEATSCGCGHSFTVK"
     gene            3412..3954
                     /gene="hscB"
                     /locus_tag="VFQ35_28925"
     CDS             3412..3954
                     /gene="hscB"
                     /locus_tag="VFQ35_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HET9934776.1"
                     /translation="MADPFELLGLEARFELDLAALEARQRELSRALHPDRYAGRGSAE
                     RRQALSRAIEVNDAVRLMKDPLRRAEALLAKHGVQLHETDAGAQPSQAFLMDVLELRE
                     ELGNAQRRADLAKVGRLAQGFKESETKSLADLAQRFAALPPNQNWSWAEVEPITHKLG
                     ELRYLRRLLDEANAIQDELG"
     gene            3998..5917
                     /gene="hscA"
                     /locus_tag="VFQ35_28930"
     CDS             3998..5917
                     /gene="hscA"
                     /locus_tag="VFQ35_28930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HET9934777.1"
                     /translation="MTLLDIFDPKAPPKAIGIDLGTTNSIVASVRDARPLALTTCDGS
                     ALLPSVVYYSSEGPILVGRNAKAHATREPLRTIQSVKRFMGRAADDPETRKLGAYRFV
                     SAANAEDGKVVRFDLGNARPSAVEVSAEILKSLKQSAIDQLRSVGGAVITVPAYFDDA
                     QRQATKDAGRLAGLEVLRLLNEPTAAALAYGLEKQQNGLFAVYDLGGGTFDVTILRLE
                     DGVFQVKSTGGDSALGGDDMDRAIAERMLSHMRATEAAQRSPELVSVVLATAREAKEA
                     LTERLEVEVELPQPGGGTTVFRLTRAELDELIQPLLERTGRACRRALRDAGLDASELS
                     GVILVGGATRVPRVRSYVKELFGKEPLADIDPDLVVAYGAALQAEMLSKASDDVLLLD
                     VLPLSLGIETMGGGVDQILPRNTTIPAGARASFTTYADNQTGFELHVVQGERELAKDC
                     RSLARFTLRGIPPMPAGMARLDVTFNVDENNLLSVTAIERTTGITQRIDVKPSYGLTD
                     EEVERMLEDALDHGEEDFEARRLSDARVEAERVLLATKKALAADADLLAGEERARIES
                     AIADLERAVREAKNASTVTVKTDALAEATHEFAGKRMNRAIAAAIAGKAVGDVEKRVE
                     HAAGIEAHLAEKGLS"
     gene            5917..6258
                     /locus_tag="VFQ35_28935"
     CDS             5917..6258
                     /locus_tag="VFQ35_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9934778.1"
                     /translation="MARVKFLDQGIEAEVAVGQSILNAAQASHAPEGSACGGVCACST
                     CHVYVEKGAELLSDADEDEEDILDKAFDVRMSSRLGCQAKILREGLIEVRISKESLDA
                     YYNEHPKETRP"
     gene            6771..8258
                     /locus_tag="VFQ35_28940"
     CDS             6771..8258
                     /locus_tag="VFQ35_28940"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9934779.1"
                     /translation="MRNAFALGMAALPLLLAYGRAASAEPLRLHGTVAAARATGGFQS
                     DEYGWGVGVLPALELPLTRALGAQAELSAIWLSEAEPPKNPNIAPEGQASGYSLAIGP
                     RLRPFAASHDGTAISPAGFWLSGAAGLELTNALWRPMVDAQIGYDFFDSRGRFGVGPM
                     LGYFHVFQSNDNVRPDDANLLLAGVHVMFDPGTKPPARKDRDGDGLYDDEDACPDQAE
                     DKDGFEDGDGCPDLDDDKDGIPDSTDHCPRVPEDKDGFEDTDGCPDPDNDKDGILDAE
                     DLCPFEPKTVNGYADSDGCPDEDQVRVLGDKIVLDDRVHFMVNSHIIRRESFSLLARL
                     AKLIKDHPEYVHVSIEGHADERGAEDFNQKLSENRASAVMEFLVKSGVPASRLSSKGY
                     GASRPLVDRKSELAYFHNRRVEFIITREGRGSTGPLLNPLKPGVLGSDGPPLPEADQP
                     EQPKQTSPVLPSAPQATPPAPPPPPPPPPPAAPKAAPSGATGGKP"
     gene            8255..9646
                     /locus_tag="VFQ35_28945"
     CDS             8255..9646
                     /locus_tag="VFQ35_28945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934780.1"
                     /translation="MSKRSKVQSAFLCGVLGVWLPAACDTGGLVGGDCKTGRIVCDGE
                     CVDPQTDPNNCGKCGNQCAPGLICSAADCVAPGGSGGAGSGGTGGVTSGGTGGIVSQA
                     GAGAGGEGAIGTGGGAGESSGGNQNQGGSGGTTPSGGTSGGPTAGSGGEGGEFNPCVP
                     PFKSADHCGDCNTQCVQPDPICSPDGGGGYHCVPVCLEPLQLCDSRCVDFNTDSDHCG
                     RCYNPCPSGICSGGKCVGASPGHVAVYCMDYTWAQDQTSHTTLLGNATIFLPRRGRVR
                     ILGFTTWAPANVRNQVNKVIGWSASALTKTYTLDTITDPATLSSRLNITDYDVFLVYE
                     QSDAPPGELATLGGGWAQTLESFAQAGGVVVVLDGAQGVKEMPDFISSAGLLPVIGHE
                     PITMEDTDTQFYVRAPADQLATMVLSPMSPKPYSCVFDLGGPPPSDVSYVVTDAPAPM
                     SGRPVVIHRVVSR"
     gene            complement(9689..9964)
                     /locus_tag="VFQ35_28950"
     CDS             complement(9689..9964)
                     /locus_tag="VFQ35_28950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934781.1"
                     /translation="MIGLFALLGFAYVPLGSRTGLEHSVALIRTPAAREAIAGLVGGV
                     VRAKDKLTQTLFPEVRESLPLPLPTAEGRPVKPLVPRLEPPHHEAAR"
     gene            10329..10796
                     /locus_tag="VFQ35_28955"
     CDS             10329..10796
                     /locus_tag="VFQ35_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9934782.1"
                     /translation="MRLFVGNLAYSTTEDRLRQEFERFGNVTSVAVVIDRMSGRSRGF
                     GFVEFSTSEEGQAAMSELNGTSLDGREIVVNEARERGAGGGGGGGGGGARPRRSGGGG
                     GGRRFEGGGGGGYGGGDYGGGGGDYGGGGGYGGGGFDRGGGGGGRGGRRDRRR"
     gene            complement(10798..>11948)
                     /locus_tag="VFQ35_28960"
     CDS             complement(10798..>11948)
                     /locus_tag="VFQ35_28960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934783.1"
                     /translation="GVVSTLLCWWAARLLDFSRASAFIGAAICAVLPYSAYLGAAPVP
                     EAPCAGLIVFAAATLVHSGRVRLLGAAALFAACASRYEAWGVALPFAAFTGWEAWRKR
                     DRTLAAAALGGLAFPVLWLLHGIVRHGDATFFVARVSAYRAALGPQDAILERLVRTPR
                     ALLLEEPVLGAVFIALTAFSLLVARVENLPKARFALVVFGVVLLTWLGDIRGSAPTHH
                     PGRALLAAWFAMALLSSELYLRALPCPARRTLALPVVGGALLVGMRFSRALQTTFVDR
                     RDAVAIGAAARQRAFARLAIDTDDYAFFAVQAAFGHPSETLVLDDRDPRKPRAQPWTN
                     DPQKLLRTLRDSRVSALVVPRTRAPFAHAVGSVSEENSGFSLIELTQR"
BASE COUNT         2192 a         3663 c         4123 g         1970 t
ORIGIN      
        1 gcttgaggcc taacgcccgc cgtgtaccgc gatatcgcag cacaaaacca aaccgcggaa
       61 ccgagtcgtt ctcgattccg cgggtttttg gttgcgcggg gaggatttga acctccgacc
      121 ttcgggttat gagcccgacg agctaccagg ctgctccacc gcgcgtcaga ggggcgggaa
      181 catatcctct ggcgccgccc agtcaaggag tgatttcggt aatttgcgaa gtcggcccgg
      241 ccgttcgcgg atgcagtcaa cgattgggca gaagagccgt ttgtacgagc gggtgcgggt
      301 tggcacatcg gggaaacgcc gtggtgaccg ggagcgcagc aatgttccag gtgggcagcg
      361 aagttccgtt gcggaaccct gacgcatgcg ttagggttag gcttacatct gaccccgatg
      421 tcgacccgcg aaaagcccct ccgtttagct gttttcccgc ctgaggtact gggcggggag
      481 ggggcgtgta cgggagcgag cgcgagcgcc gccgcaagcc tgacgggcga agcgacgggt
      541 tcgagcaatc cggatagttg gcttcaggtg ggtgagctcg cgaaggcaac cggaaagacg
      601 gtgcgggcga ttcacctcta tgaggagctc gggctgcttc ggacgcacgt gcggtcgaag
      661 gggcggtacc ggctgttcac ggaggaggcg gtggttcggg tgcgctggat cgtgaagatg
      721 cagagcctcg ggctgtcgct gaccgagatt caggagctgg ttcgggagca agaggcttcc
      781 gggagcgcgc agtttgctgc ctctcggttg cgcgaggtgt accggacgaa gctcgctgag
      841 acgcggaaca agatgagtga gcttcgggcc ctcgaggccg agctcgaaga gagcctcgcg
      901 tatttggatg cgtgcgacac ggcgtgtcag tcgacgttgc cggtgcattc gtgtccgagc
      961 tgtgagcggc acccggagcg accgaacgct cccgatttgg tggccggcgt gcacgtgaac
     1021 tgacgtgaac tgacgtgcgc cgacgagtcg acgtgaactg gcgtgaaccg acgtaaccga
     1081 cgcgagctga cgtaactgaa gcgaaccgac gtggattgaa gagagatcgg cgaatggcac
     1141 tgaagtttcc catctacatg gacaatcacg cgacgacccc gatggaccct cgggtgctcg
     1201 aagcgatgtt gcccttcttc acgcaagagt tcggtaacgc tgcgagccgc acgcacgcct
     1261 acgggtggca cgcggaagcg gcggtggact cggcgcgcga gacggtcgcg aacttcatca
     1321 atgccgagag cggcaaggag attgtgttca cgtcgggtgc gacggagagc gacaatctcg
     1381 cgatcaaggg cgtcgctgag tattacggcg gcaagggcaa gcacatcgtg acgacgacga
     1441 tcgagcacaa ggcggtgctc gattcgtgca agcgcctcga gaagcagggc ttcgaagtga
     1501 cgtacgtgag ccccggccaa gatggtctgg tggatccggc cgacattcag aaagcgctca
     1561 cggagaagac gattttggtg agtgtgatgc tcgccaacaa cgaggtcggg acgattcagc
     1621 cgatcgaaga gattggcgcg atcactcggg cgcgcggcgt gctgttccac gtggatgcgg
     1681 tgcaaggcgt tggcaagacg ccgttcgacg tgcgggcgat gaacgtggac ttggcgtcgc
     1741 tcaccgcgca caagatttac gggccgaagg gcatcggcgc gttgtacgtg cggcgcagca
     1801 agcctcgcgt gcgcttggtt gcgcagatgg acggcggtgg tcacgagcgc ggcaatcgtt
     1861 cgggcacgtt gaacgtgccg ggcatcgtcg ggttcgggaa ggcggtcgag attctgcggc
     1921 aggaaggtga agccgaggcg ctgcggctcc gcgggctgcg cgaccggctg tggaagcggc
     1981 tgacgagcga gctcgaccag gtgattttga acggaagcgc ggagcgccgg cttccgggca
     2041 acttgaattg ctcttttgcc ttcgtcgaag gtgaggggct gatgatggcg atcaaggacg
     2101 tcgccgtttc gagcggctcg gcgtgcacga gcgcgagcct cgaaccttct tacgtgcttc
     2161 gttcgatggg cctcgacgag gacctcgccc attcttcgat ccgcttcggc atcgggcgtt
     2221 tcaacaccga ggaagaagtg gattacgtcg gtgacctggt cgcgagccgc gtgaagaagc
     2281 tccgcgacat gtcgccgctc tgggagatgc accaagccgg catcgacatc aaatcaatcg
     2341 aatgggtggc acattgagca atccctcccc gccgacgttt cgaggatcga acgagacgaa
     2401 ggctcatctg ctcgccacaa acttctgaag atctctggag aacctaaatg gcctacagcg
     2461 aaaaagtcat cgagcactac gagaaccccc gcaacgtcgg tgcgctcgac aaggacgatc
     2521 cttcggttgg gaccggtctc gtgggcgccc ccgcctgcgg cgacgtgatg cgcttgcaga
     2581 tccgcgtgaa cgacgcgggt gtcatcgaag atgcgaagtt caagaccttc gggtgcggct
     2641 cggcgatcgc gtcgtcgtcg ctcgcgaccg agtggatcaa gggcaagagc atcgaagagg
     2701 ccgcgggcgt gaagaacagc caaatcgccg aagagctgaa cctgccgccg gtgaagattc
     2761 actgctcggt gctggccgag gacgccatca agagcgccat cgaggactac aagaagaagc
     2821 gcgcggcgcg cgaggcgctg gagagcgggc ggtcatgagc atggccgcgc cggagtcgat
     2881 cggtccgcgg gcgtcggagt cgccgacggt gatgaacgtc gccgggccgg actccgcggg
     2941 ttcgcgtgtt tccgaggcgc cgcagtcggt ggctccggcc tcggtgagcc ccaggtcgga
     3001 agcgccggtg ccttcgagca agcgcacgat tttcgtgagc gaccaggcgg tggtgttcgc
     3061 gaagcagaag ctcgagcagc gcggcacgcc gcacgcctcc gttcgcctcg gtattcgggg
     3121 tggcgggtgt tcggggttct cttacgtcat ccagttcgag gacgaggcgc cgcgcgagcg
     3181 cgacaacgtg ttcgaatatg gcggcgtgcg cttcgtgatc gacaagaaga gcttgatcta
     3241 cttggccggt tcgaccctcg attacgaaaa aacactgatg ttccagggtt tcaagttccg
     3301 aaacccgaac gaggccacga gctgcggctg cgggcactcg ttcacggtga agtagtcgcg
     3361 ctgcaaagcg cgcgatgagc ctatcgctcg acagacccga agctgcgtgc cttggctgac
     3421 cctttcgaac ttctggggct cgaagcgcgc ttcgagctcg atctcgccgc cctcgaggct
     3481 cggcagcgtg agctgagccg cgcgttgcac cccgatcgct acgcggggcg cggttcggcg
     3541 gagcggcgac aagcgctgtc gcgggcgatc gaagtgaacg acgcggtgcg cttgatgaag
     3601 gatccgctgc gccgcgcgga ggcgttgctg gcgaagcacg gtgtgcagct ccacgagacc
     3661 gacgccggcg cgcagccgtc gcaggcgttt ctgatggacg ttctcgagct ccgcgaagag
     3721 ctcggcaatg cgcagcggcg tgcggatctc gcgaaggttg gacggctggc gcagggcttc
     3781 aaagaaagcg aaacgaagtc gctcgccgac ttggcccagc gcttcgcggc gctccccccc
     3841 aaccaaaatt ggagctgggc ggaagtggaa cccatcacac acaagctcgg cgaattgcgc
     3901 tatttgcggc gcttgctcga cgaagccaac gccattcaag acgaactcgg ctgaaggcag
     3961 cgcgcgactc cgcgccgcca gctgacagga aacgcacgtg actctgctcg acatcttcga
     4021 tcccaaagcg cctccgaaag cgatcggcat cgatctcgga acgacgaatt ccatcgttgc
     4081 gtcagtgcgc gacgcgcgtc ctctggcgtt gacgacgtgc gacggctccg cgctgttgcc
     4141 gtcggtcgtt tactattcga gcgaagggcc cattctggtg gggcgcaatg cgaaggcgca
     4201 tgcgacgcgc gagcccctgc gcacgattca aagcgtgaag cgcttcatgg ggcgcgccgc
     4261 ggacgatccc gagacgcgaa agctcggggc ctatcgcttc gtttccgccg ccaatgcgga
     4321 ggatggcaag gtcgtgcgct tcgatctcgg caacgcgcgg ccgagcgcgg tcgaggtgag
     4381 cgccgagatc ttgaagagct tgaagcagag cgccatcgat cagctgcgca gcgtgggcgg
     4441 cgcggtgatc acggtgccgg cgtacttcga cgacgcgcag cgacaggcaa ccaaagacgc
     4501 ggggcgcttg gccgggctcg aggtgctgcg gctcttgaac gagccgaccg cggccgcgct
     4561 cgcttacgga ctcgaaaagc agcaaaacgg cttgttcgcc gtgtacgacc tcggcggcgg
     4621 caccttcgac gtcacgattt tgcggctcga ggacggcgtt ttccaggtga agagcacggg
     4681 cggcgacagc gcgctcggcg gcgacgacat ggatcgcgcc atcgcggaac ggatgctctc
     4741 gcacatgcgc gcgacggagg ccgcgcagcg ctcgccggag ctcgtgagcg tcgtgttggc
     4801 gaccgcgcgc gaagcgaagg aagctttgac ggagcgcctc gaagtcgagg tcgagttgcc
     4861 gcaaccagga ggcggcacga ccgtgttccg actcacgcgc gccgagctcg acgagttgat
     4921 tcaaccgttg ctcgagcgta cgggacgcgc ttgccggcgt gcgttgcgcg acgctgggct
     4981 cgatgcgtcg gagctttccg gggtaatttt ggtggggggc gcgacgcggg taccacgcgt
     5041 tcgcagctac gtgaaagagc tgttcggcaa agagcctctc gccgacatcg atccggatct
     5101 cgtcgtggcc tacggcgccg cgctgcaagc cgaaatgctg tcgaaggcga gcgacgacgt
     5161 cctcttgctc gacgtgctcc cgctctcgct cggtatcgaa accatgggcg gcggcgtcga
     5221 ccaaatcttg ccccgcaaca cgacgatccc cgccggtgcg cgcgccagtt tcacgaccta
     5281 cgccgataac caaaccgggt tcgagcttca cgtcgtccaa ggcgaacgcg agctcgccaa
     5341 ggactgtcgc tcactcgctc gctttacctt gcgcggcatt cccccaatgc ccgccggcat
     5401 ggcccgcctc gacgtcacct tcaacgtcga cgaaaacaac cttctcagcg tgaccgccat
     5461 cgagcgcacg actggcatca ctcagcgcat cgacgtgaag ccgagctacg gcctgaccga
     5521 cgaagaagtc gagcgcatgc tcgaagacgc actcgaccac ggcgaagaag acttcgaggc
     5581 gcgccggctc agcgacgccc gcgtcgaagc cgagcgcgtg ctcctcgcca ccaaaaaagc
     5641 gctcgccgcc gacgcggacc tcctcgccgg cgaagagcgc gcccgcatcg aaagtgccat
     5701 cgccgacctc gagcgcgccg tgcgcgaagc caagaacgca agcaccgtca ccgtgaaaac
     5761 cgacgcgctc gccgaagcga ctcacgagtt tgccggcaag cgcatgaacc gcgcaatcgc
     5821 cgccgccatc gctggcaagg ccgtcggcga cgtagaaaag cgcgtcgagc acgccgccgg
     5881 tatcgaagcg cacctcgccg aaaaaggtct cagctgatgg cacgagtgaa gttcctcgat
     5941 cagggcatcg aagccgaagt ggccgtcggc cagagtattc tcaatgcggc ccaagcgagc
     6001 cacgctcccg aaggcagcgc gtgcggcggc gtctgcgctt gctcgacctg ccacgtctac
     6061 gtggagaaag gcgccgagct cctgagcgat gccgacgaag acgaagaaga cattctcgac
     6121 aaggcgttcg acgtgcgcat gagctcgcgt ctcggctgcc aagcgaagat acttcgcgaa
     6181 ggccttatcg aagtgcgaat ctccaaagag agcctcgacg cctactacaa cgagcacccc
     6241 aaagagacgc gcccgtagcc gccgcgcacg ccgcacgttc caccgccgcg tcagcctctc
     6301 gctcgcggtg ccacgtatgc gctcaggtac gatgcggcag ctgcgcagga acatcgttcg
     6361 cgagtagatc gaggggcgat tgtgcttgcg aaccgcgcga gcgcgatctg cacgcgttcg
     6421 cggactctgg cgcgtgatcg agcagttcac ctggcgcaat tcgacagctc ggaattgttg
     6481 cggtgttggc gagggtcggc ttgaatgttc gctcgagggt cgactgcacg gcgcggcgag
     6541 cgggtttctc atccgctgcc gagaccggtc gcgcgcgaag ggactcgcaa cagatgaacc
     6601 gaaagctgga gcggcgtaaa ccgagtcact tggcgcgcga atcggcgcaa ttcgtcgggt
     6661 atttgcggcc atgcgggcac gtggtcagcc gttcggttga gtcatctgtc tcgaggcggg
     6721 acttattgcc gtacgtgcgt tgccgttctc ggggggcggg acaccctcgc atgcgtaacg
     6781 cattcgctct tggaatggcg gctttgccgc tgctgctcgc gtacggacgt gcggcctccg
     6841 cagagccgct ccgccttcac gggacggtcg cggccgcacg cgcaaccggc ggctttcaat
     6901 cggacgagta cggttggggc gtgggcgtgt tgcctgctct cgagctgccg ttgacgcgtg
     6961 cgctcggcgc acaggcggag ctctccgcga tttggttgag cgaagcggag cctccgaaga
     7021 acccgaacat cgcgcccgaa gggcaggcga gcggttatag cttggcaatt ggtccgcgcc
     7081 tgcggccgtt tgccgcttcg cacgatggga ctgcgatctc gccggccggg ttttggttgt
     7141 cgggtgcggc aggcttagag ctcacgaacg cgttgtggcg acccatggtc gacgctcaga
     7201 ttggctacga tttcttcgat agccgtgggc gcttcggagt cggcccgatg ctgggatact
     7261 ttcacgtgtt tcagtcgaac gacaacgtgc gacccgacga cgcgaacctc ctcttggcgg
     7321 gcgtgcacgt gatgttcgat cccggcacga agcctcccgc tcgtaaggat cgcgacggcg
     7381 atgggctcta cgacgacgaa gacgcgtgcc ccgatcaagc cgaggacaag gacggcttcg
     7441 aagacggcga cggttgtccc gatctcgatg atgacaagga cgggatcccg gactctacgg
     7501 atcattgtcc ccgggtcccc gaagacaagg acggcttcga agatacggac ggctgtcccg
     7561 atcccgacaa cgacaaggac ggcatcctcg acgccgagga tttgtgcccg ttcgagccca
     7621 aaacggtgaa tggctacgcc gactccgacg gttgccccga tgaagatcaa gtgcgcgttc
     7681 tcggtgacaa gatcgtgctc gacgatcggg ttcacttcat ggtgaacagc cacatcattc
     7741 ggcgcgagag cttcagtttg ctcgcacggc tcgcgaagct catcaaagat caccccgagt
     7801 acgtgcacgt ctcgatcgaa ggccacgcgg acgagcgcgg cgccgaagac ttcaaccaga
     7861 agctcagcga gaatcgcgcc tcggcggtga tggagttctt ggtgaagagc ggcgtgcccg
     7921 cttcgcgcct gagctcgaag gggtacggcg cctcgcgccc gctcgtcgat cgcaagagcg
     7981 agctcgcata cttccacaat cgccgcgtcg agttcatcat cacgcgcgaa ggccgaggct
     8041 cgacggggcc gctgctcaat ccgctcaagc caggcgtgct cggcagcgat gggccaccct
     8101 tgcccgaagc ggaccaaccg gagcaaccga aacagacttc acccgtgctt ccgagcgcgc
     8161 cgcaagcgac gcctccagcg ccgccaccgc caccaccgcc tccgcccccc gcggcgccga
     8221 aggccgctcc gtccggagcc acgggaggta agccatgagc aagcgatcga aggttcaaag
     8281 tgccttcttg tgcggcgtgc tgggcgtttg gctccccgcc gcgtgcgaca cgggcgggct
     8341 cgtcggcggc gactgcaaga cgggacgcat cgtttgcgac ggcgagtgcg tcgaccctca
     8401 aacggatccg aacaactgcg gtaagtgcgg caaccagtgc gcgcccgggc tcatttgctc
     8461 ggctgctgat tgcgtggctc caggcggtag cggcggcgcc ggcagcggcg gcaccggagg
     8521 cgtgacgagc ggcggtaccg ggggcatcgt cagtcaagcg ggcgctggcg cgggtggcga
     8581 aggcgcgatc ggcactggag gcggtgcggg cgagtcgtct ggaggaaacc agaaccaggg
     8641 cggcagcggt ggaacgactc cgagtggtgg cacttcgggc ggcccgaccg cgggtagcgg
     8701 tggcgaaggc ggagagttca atccatgcgt tccgccgttc aagagcgcgg accactgcgg
     8761 cgactgcaac acgcagtgcg tgcagccgga cccgatttgc tcaccggacg gcggcggtgg
     8821 ctatcactgc gtcccggtgt gtctcgagcc tcttcagctc tgcgatagcc ggtgcgtcga
     8881 cttcaacacc gactcggatc actgcgggcg ctgctacaat ccgtgtccga gcggcatctg
     8941 ctccggcggc aagtgcgtcg gcgcaagtcc cggacacgtc gcggtgtact gcatggatta
     9001 cacctgggcg caggaccaga cgtcgcacac gacgctgctc ggcaacgcca cgatcttttt
     9061 gccgcgacga ggccgcgttc gcatcttggg cttcactacg tgggcgcccg ccaacgtgcg
     9121 caatcaagtc aacaaggtga tcggctggtc ggcgagcgcg ctcaccaaga cgtacacgct
     9181 cgacacgatc acggatcctg cgactctgtc gtcgcgcttg aacatcaccg actacgacgt
     9241 gttcctcgta tacgagcaat cggacgctcc tccgggagag ctcgcgacgc tgggcggcgg
     9301 ttgggcacag acgctcgagt ccttcgcgca agcaggcggc gtcgtggtcg tgctcgacgg
     9361 cgcgcagggc gtgaaagaga tgcccgactt catcagttct gctgggctgc ttccggtgat
     9421 cgggcacgag ccgatcacga tggaagacac cgacacgcag ttttacgtgc gcgcgccggc
     9481 tgatcagctc gcgacgatgg tgctctctcc catgtcgccg aagccttaca gttgcgtctt
     9541 cgatctcggg ggcccgccgc cgagcgacgt ctcgtacgtg gtcactgacg cgcctgcacc
     9601 catgtccggc aggcccgtcg tgatccatcg cgtggtctcg cgctagcatc gcggcgaggc
     9661 ggcaacgcgc gctgcgctcg atcgcgagtc agcgcgcggc ttcgtggtga ggcggctcga
     9721 ggcgaggcac gagcggcttc acgggtctgc cttcagccgt gggcaacgga agcggcaagc
     9781 tctcgcggac ctcggggaag agcgtctggg tcaatttgtc cttggcgcgc accacgccgc
     9841 cgacgagccc agcgatggct tctcgcgccg ccggcgttcg gatgagcgcg acgctgtgct
     9901 cgagccccgt cctcgatccg agcggcacgt aggcgaatcc gagcagcgcg aacaagccga
     9961 tcaccgactc taccgcacgt ctacctacat ttccgagcac ggcggcaggg tacgtgagcc
    10021 gccagcattc ggaaaagttt tgtgcaaaac cgcgcccgga gcggggggct gaagccccgt
    10081 cgcgtgattg tcttcatgcg tacataggac cttcggccga atctctgggc gagcaaaatg
    10141 aaaactggta tgctcgccag cgccctttcg ccaggttccg aagttcgtcc ggtcgatccg
    10201 ctcgagtgta ggtctgtggt ggtggccgct tggatttgag gcggctggta gtgagcgtca
    10261 gcgtcgagcg ttcgttcaag ggccggaaga cgcgatcgat agagatagag agagcaagga
    10321 gagttcgtat gcgccttttc gttggcaacc tggcctactc gaccaccgag gaccgtctgc
    10381 gccaggagtt cgagcgtttt ggaaacgtga cgtcggtcgc ggtcgtgatt gatcgcatga
    10441 gcggtcgttc gcgaggtttt ggtttcgtag agttctcgac ctcggaagag ggtcaggccg
    10501 ccatgagcga gctaaacggc acgtcgctcg acgggcgcga gatcgtggtc aacgaggcgc
    10561 gtgagcgtgg cgccggcggc ggcggtggcg gtggcggcgg tggtgcgcgt ccgcgtcgct
    10621 ccggtggcgg cggcggtggt cgtcgcttcg aaggcggcgg cggcggcggc tacggcggcg
    10681 gcgactacgg cggcggcggc ggtgactacg gcggtggcgg cggctacggc ggcggcggtt
    10741 tcgatcgcgg cggcggtggt ggtggtcgcg gcggtcgtcg cgatcgtcgt cgttgattca
    10801 gcgctgagtc agctcgatta gagaaaatcc ggagttctct tcgctcacgc tgccgacggc
    10861 gtgagcgaag ggagcacggg tacggggaac gacgagcgca gagacgcggg agtctctcaa
    10921 tgttcgcagc agcttttggg gatcgttggt ccacggctgc gcgcgtggtt tccgcggatc
    10981 gcggtcgtcg agcacgagcg tctcgctcgg atggccaaat gcggcctgaa cggcgaagaa
    11041 agcgtagtcg tcggtgtcga ttgcgaggcg cgcgaaggcg cgctggcgag cggccgcgcc
    11101 gattgcgacc gcgtcgcgtc gatcgacgaa cgttgtctgc aaggcgcggc tgaatcgcat
    11161 gccgacgagc agcgcgcccc ccacgacggg gagcgcgagc gtgcgacgtg cgggacaagg
    11221 caacgcgcgc aggtagagct cgctcgagag caaggccatc gcgaaccacg ccgctaagag
    11281 ggcgcgtccg ggatggtgcg tcggcgccga gccccgaata tcgccgagcc aggtgagcag
    11341 cacgacgccg aacacgacca gcgcgaagcg cgccttcgga aggttctcga cgcgtgcgac
    11401 gagcaagctg aacgcggtga gcgcgatgaa gacggcgccg agcaccggct cttctaagag
    11461 cagcgcgcgc ggtgttcgca cgaggcgctc cagtatcgcg tcttgaggcc cgagcgcggc
    11521 acgatacgcg ctgacgcgcg cgacgaagaa cgtcgcgtcg ccatgacgca cgatgccgtg
    11581 cagcagccac agcaccggaa acgccaatcc tccgagcgcg gcggcggcca gagtccggtc
    11641 gcgcttgcgc catgcttccc agccggtgaa cgccgcgaac ggcagcgcga cgccccaagc
    11701 ttcgtagcgc gacgcgcacg ccgcaaacag cgctgccgcg ccgagtaaac gcacgcgccc
    11761 cgaatgcacg agcgtcgcgg ccgcaaatac gatgagcccg gcgcacggcg cctctggtac
    11821 cggcgccgct ccgaggtagg ccgagtaggg gagaacggcg cagatggcgg cgcctatgaa
    11881 cgcggacgca cgcgaaaaat cgagcaggcg cgccgcccac caacacaaca aggtgctcac
    11941 gacgccga
//
LOCUS       DASTKS010001348         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_890865, whole genome shotgun sequence.
ACCESSION   DASTKS010001348 DASTKS010000000
VERSION     DASTKS010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890865"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFQ35_28965"
     CDS             <1..1017
                     /locus_tag="VFQ35_28965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934784.1"
                     /translation="SAEPRRSAVAVVSVDTDRALLTRIGAEIGALGWRVKTPPARPDL
                     ALAQIAKRAQTLAVLRIASRGEGIELWVAPEVDAEAHSEWIELDRRHPEVGVLRAIES
                     LRARFVELGIEPEAAPAEDPSAADGEPLASEPGSKLNAAESARSHAALQEEGAFETEP
                     PSSLPPRGVELGLGAGALYASRAASLESKLALAIRIVPDPHFVTSLEAWLPLSEVNVA
                     SGERSAALRSTLFGATGEYRGRLGRLRWGAGLGCGLGVVQLRGHSSEGFVGLSKTLYA
                     AVPWVRFQSEFPLSPRFALRLQATAGIARPRPVVQLGDVVGDYWGQPMLSATGSLEWA
                     PFAK"
     gene            complement(1022..1369)
                     /locus_tag="VFQ35_28970"
     CDS             complement(1022..1369)
                     /locus_tag="VFQ35_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9934785.1"
                     /translation="MLAEDIQKLRKELSCTVKELATTLGVEPKEVTAWEAGELFPTKR
                     HVDAMNALRARGKESIVRAPRGKAAQQKTGQQRLADPALWRVVRKLVEHPALFDEVLK
                     LSEKYGDPAEPAS"
     gene            1495..>2193
                     /locus_tag="VFQ35_28975"
     CDS             1495..>2193
                     /locus_tag="VFQ35_28975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934786.1"
                     /translation="MRREVGLFAMVVATLGAFAGCLPKPEPSASELPVTRAAPLADDG
                     VERVARFSVTLHGSILDPDDAWLFRGALSDYHVGRINRRELPETLSERRVAVNAWFDP
                     ELAQTWIAPVAELAAGEVYSLAAPGWGRLAQVTVRAESTLPLLKRLWPPAGRAGGSVA
                     LYCGAIDAIEPAVLELEPSHLRAQVIGVVGSLSSGERCVELDFDDVSLPLEWQLPPPL
                     AASVLWDSLPLEFQP"
BASE COUNT          306 a          718 c          764 g          405 t
ORIGIN      
        1 agcgccgagc cgcgtcgaag cgcagtggcc gtggtgtcgg tcgatacgga tcgcgccctt
       61 ctgacccgga ttggcgccga gattggcgct ctcggttggc gagtgaagac tccaccggca
      121 cgcccggacc tcgccctcgc ccagatcgcg aagcgcgcac agaccttggc ggtgttgcgc
      181 attgcctcgc gtggggaagg catcgagctg tgggttgcgc ccgaggtcga cgcggaggct
      241 catagcgaat ggatcgagct cgaccgacgg catccggaag tgggtgttct gcgcgccatc
      301 gagtccctgc gggcgcgatt cgtcgagctc gggatcgagc ctgaggcggc gcctgccgaa
      361 gacccgtccg cggccgatgg cgagcctttg gcgagcgagc ccgggagcaa gctgaacgcg
      421 gccgaatccg cacggtcgca cgccgcgctg caggaagagg gcgcgttcga aaccgaaccg
      481 ccttcgtcgc tgccaccgcg cggggtcgag ctcggactcg gggcgggcgc gctgtacgcg
      541 agccgcgcgg cctcgctcga aagcaagttg gctctcgcga tccgaatcgt ccctgacccg
      601 catttcgtta ccagcctcga ggcgtggctg ccccttagtg aggtgaacgt tgcgagcggc
      661 gagcgctcgg cggcgctacg cagcacgcta ttcggcgcta ccggtgagta tcgcggtcgc
      721 ctcggccggt tgcgctgggg agctgggctc ggttgcgggc tcggagtcgt tcagctgcgc
      781 ggtcattcga gcgagggatt cgtcgggcta tcgaagacac tctacgcagc ggtgccctgg
      841 gtgcggtttc agtccgagtt cccgctgtct ccacgattcg cgcttcgact tcaggcaaca
      901 gcggggatcg cgcgaccccg cccggtggta cagctcggcg acgtcgtcgg cgactactgg
      961 gggcagccga tgctttcggc cacgggaagc ctcgaatggg cgccgttcgc gaagtgagct
     1021 gtcagctcgc cggctccgcc gggtcgccgt acttctccga gagcttcagt acttcgtcga
     1081 atagcgcggg gtgctccacc aatttccgga ctacccgcca aagagcgggg tctgcgaggc
     1141 gctgttgtcc cgtcttctgc tgcgcggctt ttccacgggg cgcgcgtacg atcgattctt
     1201 tcccacgtgc ccggagagcg ttcatggcat cgacgtggcg tttggtgggg aagagctcgc
     1261 cagcctccca cgcagtcact tccttgggct ccacgccgag cgtggtagcg agctctttca
     1321 ccgtgcacga gagctccttg cggagctttt gaatgtcttc agcgagcacg ggcgcagcat
     1381 atcactctcg gcggcgccag tggcgcggtc ggcgttgcgg gcagggcgag catcgaatcg
     1441 gggtctcgct gagttttccc gcaactcgct ccggcggcga ccgactcgag caccatgcgt
     1501 cgtgaagtcg ggctcttcgc catggtcgtt gccactctcg gggcattcgc ggggtgcctg
     1561 ccgaaaccgg agccgagcgc ttcggagctc ccggtcaccc gcgctgcgcc gcttgccgac
     1621 gatggcgtcg aacgggtcgc ccggttctcg gtgacgctac acggctcgat cttggacccg
     1681 gacgacgctt ggctctttcg cggagcgctc agtgactatc acgtggggcg catcaatcgg
     1741 cgcgagctgc cggagacgct cagcgaacgc cgagtcgccg tgaatgcttg gttcgacccg
     1801 gagctcgcac aaacttggat cgctcccgtc gccgagctcg cggcggggga ggtgtacagc
     1861 cttgccgcgc cgggttgggg caggctcgcg caagtgaccg tgcgagctga gtcgactctg
     1921 cctctactga agcgcttatg gccaccggct ggccgcgcgg gcggctccgt ggccctctac
     1981 tgcggagcga tcgacgcgat cgagcccgcc gtgctcgaac tcgagccgtc gcacctgcgc
     2041 gcgcaggtca tcggagtcgt cgggagcctt tcgagcggtg aacgctgtgt cgagctggac
     2101 ttcgacgacg tttccctgcc gctcgagtgg caactccctc cgcctctggc tgcttcggtc
     2161 ctgtgggact ccctgccgct cgagtttcag ccg
//
LOCUS       DASTKS010001349         6161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_890954, whole genome shotgun sequence.
ACCESSION   DASTKS010001349 DASTKS010000000
VERSION     DASTKS010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890954"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFQ35_28980"
     CDS             complement(<1..455)
                     /locus_tag="VFQ35_28980"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9934787.1"
                     /translation="MSNNHEIHSDALVLFGATGDLAFKKIFPALYSMFKRGHAPFPIV
                     GVSRGGSSLEDLKQRVRQSITELGGTLDERALGVLTESLRYVDGDYRTLELFQKVKQT
                     LGNARRPVYYLSIPPSAFADVVEYLGRSGCSEGARVVVEKPFGRDLGSA"
     gene            complement(452..1471)
                     /gene="gnd"
                     /locus_tag="VFQ35_28985"
     CDS             complement(452..1471)
                     /gene="gnd"
                     /locus_tag="VFQ35_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET9934788.1"
                     /translation="MQIGMIGLGRMGGNMVRRLLRKGHSPCVYDLSQDSVRALAAEGA
                     TGASSLAELAEKLEAPRAIWLMVPAAHVDDTLKALVPHLKPGDIVIDGGNSHYHDDIR
                     RAAELSAQGLHYLDVGTSGGVWGLERGYCLMIGGDTGAVQRLDPIFEALAPGVEMAAR
                     TEGRSGTPTSAERGFLHCGPAGAGHFVKMVHNGIEYGLMAAFAEGLNVLRHANAGKVE
                     RTKDAETSPLRNPEHFQYDLDVASITELWRRGSVVSSWLLDLTARALASDPGLEAFAG
                     RVSDSGEGRWTVQAAVDEGVPVHVLSAALFDRFSSQGRGDFANRVLSAMRFGFGGHHE
                     KKAAT"
     gene            1788..3437
                     /locus_tag="VFQ35_28990"
     CDS             1788..3437
                     /locus_tag="VFQ35_28990"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9934789.1"
                     /translation="MRLRDERGDAAVRALLASVGIPPSAVDSDTGWLSVAATRRIMSA
                     LSTALGRPALETRGQWMTHPEVLGSYVRMLRVASSPEDAYRYLSANAAETTRVGTYEL
                     GTISVNHAELSYHRRAEAESEQNDPLLCVARQAELVSVPRIWGLSDAHVAHPRCLAEG
                     ADECLYILDWSAPNRKRWVILGCLLGGLICGGAVAISGSFIASIIAALIGFGFGGALS
                     SLSARIREERAARAFEKNRIAALERGLEAHGHFRDAAGDLAGVTLGGKYRILRKIGSG
                     GIGAVYAAEHIALGSRVAVKVLRGAAAVDASEIARLRREARVQVSIEHPNVVRVLDLD
                     QLPDGSIYVVMEQLQGRSLAARLKSGGPLSATEAVTIFRAVCKGLSAAHQLGVVHRDL
                     KPGNIFLCDDRSVKILDFGMSKFAEAESLTQDGYTLGTPEYMSPEQCIGAPVDPRTDL
                     YAFGVLMYEALVGSLPISGKNRREFLELHQRMIPPSMRERRPDLDIPAELDEAVMRCL
                     KKRASERPATARELDKLLSKVPIAGVSSVDSQPPTPSRALR"
     gene            complement(3482..4081)
                     /locus_tag="VFQ35_28995"
     CDS             complement(3482..4081)
                     /locus_tag="VFQ35_28995"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9934790.1"
                     /translation="MSTARRLHYSYAAYLRALKDSAIKLEYLDGEIYAMAGGTPAHAD
                     LSAAAIATLVRVLGQGCRVPTSDLKVRIEETDLSTFPDAVVVCGPRAVSAIDENAVIN
                     PTLLVEVTSPSTEDYDRGEKLRHYKHLSSLKAVIFVSHRRAQITIVERTPAGWAEHDV
                     NAGEEVVLNDPACRFPLDEIYRGVELGSDSNAQASGAVD"
     gene            4217..4651
                     /locus_tag="VFQ35_29000"
     CDS             4217..4651
                     /locus_tag="VFQ35_29000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934791.1"
                     /translation="MTRNLLHLATCTLGAIAMACSAADACPDEQPTRSVETGTTDQLA
                     LVYESAPWSGPLEPFPAMGSLSFKHDLGMTPKIVATYLSFSESGTGSSSVAESAGNAA
                     LIDCVDSQVIRVRNDTCEKHFFIRVVAMATPGAPTDVTCSED"
     gene            complement(4680..5393)
                     /locus_tag="VFQ35_29005"
     CDS             complement(4680..5393)
                     /locus_tag="VFQ35_29005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934792.1"
                     /translation="MANTRIISIRELPRAAPGEPNAGGAGAVATPDSLPKTRLIPMRE
                     GFAAREPAVASLPDLATGTSRANLRTTLVPVEHLLARRAPAPASAADESNAPPAPPSQ
                     ATPAPRKRSIPPKRRILVAALVGALMSSMAIAANVMGAARGTPAASQSNVQPARAAKS
                     LSKPAAPAARRREPTPPTSKLTPHETGISLERAASDAVVEGRFADAAQVYERLAEAMP
                     QRAVYREAARILRERGESR"
     gene            complement(5362..>6161)
                     /locus_tag="VFQ35_29010"
     CDS             complement(5362..>6161)
                     /locus_tag="VFQ35_29010"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HET9934793.1"
                     /translation="HLLRSGSLCEDAAQTLAALVEAKLNMVIAGGTGSGKTSLLNVLT
                     SFAPERDRVVVIEDSKEVQVQRPHVVSMEARPADEHGRGAVSIRDLFRATLRMRPDRI
                     IIGEIRGAEALDIVQAMVSGHGGCMGTLHATYPRDTLTRLETMAMMSDVNLPLTALRM
                     QIASGVNVIVQVTRMQDGSRAVSHITEVLGFDLESNRYKLRDLFARNYLGFAADGRVL
                     SELAPTGMLPSFLPHLRERGLELPESVYSKAREQENTNGKHQNHFDS"
BASE COUNT         1061 a         2067 c         1963 g         1070 t
ORIGIN      
        1 tgcgccgagc cgagatcgcg cccgaacggc ttctccacca cgacccgagc gccttcggag
       61 caaccgctgc ggcccaggta ctcgaccacg tccgcgaacg cgctcggcgg aatcgagagg
      121 taatagacgg ggcggcgcgc gttgccgagc gtttgcttca ccttctgaaa gagctcgagt
      181 gtccggtagt caccgtcgac gtagcgaagg ctctcggtga gaacgccgag cgcgcgctca
      241 tcgagcgtgc ccccgagctc ggtgatgctc tggcgcaccc gctgcttcaa atcctcgagg
      301 ctcgagccgc cgcgcgacac gccgacgatc ggaaacggcg cgtgcccgcg cttgaacatc
      361 gagtagagcg caggaaaaat cttcttgaag gcgagatcac ccgtcgcgcc gaagaggacg
      421 agggcgtccg agtggatttc gtggttgtta ctcatgtagc tgctttcttc tcgtggtggc
      481 cgccgaagcc gaagcgcatc gcggagagca cgcggttcgc aaagtcaccg cgaccttggg
      541 acgaaaaacg atcgaagagg gccgcgctga gtacgtgaac cgggacgcct tcgtccactg
      601 cggcctgcac cgtccagcgg ccctcgcccg agtcggacac gcgcccggcg aacgcctcga
      661 gcccagggtc agaagcgagc gcgcgcgccg tgagatcgag cagccaagag ctcacgacgc
      721 tgccgcggcg ccagagctcg gtgatgctcg caacgtcgag gtcgtactgg aagtgctcgg
      781 ggttacgcag cgggctggtc tcggcgtcct tggtgcgctc gactttaccc gcgttcgcgt
      841 gacgcagcac gttgagccct tccgcgaagg cggccatgag accgtactcg atgccgttgt
      901 gaaccatctt cacgaagtga cccgcgcccg ccggcccgca gtgcaagaag cctcgctcgg
      961 cgctggtcgg agttccactg cgtccttcgg tgcgcgcggc catttcgacg cccggtgcga
     1021 gcgcctcgaa gatcgggtcg agccgttgca cggcgccagt gtccccgcca atcatcaggc
     1081 agtagccgcg ctcgaggccc cagacgccgc cgctcgttcc gacgtcgaga taatggagac
     1141 cctgcgcgct gagctctgcc gcgcggcgga tgtcgtcatg atagtgggag ttaccgccgt
     1201 cgatcacgat gtcgcccggt ttcaagtggg gcacgagcgc cttcaaggtg tcgtccacgt
     1261 gcgctgccgg aaccatcagc catatcgcgc gcggcgcttc cagtttctcc gcgagttcgg
     1321 ccagcgagct cgcccccgtc gcgccttcgg ccgcgagagc gcggaccgag tcttgagaaa
     1381 ggtcgtacac gcagggggag tgtccctttc gaaggagcct ccgcaccatg ttgcctccca
     1441 ttcggccgag gccgatcatc ccaatttgca tgctgaaagc gctccttcgg gcccgaactg
     1501 catccccgcg ctcggcccgc tatccgacgg actgaaggcg gcgatgtagg gcatgctcta
     1561 gcgatagggc gattcggtcg cccgcgaaag ggtcgaggcc gcgaatctcg acagcgagcg
     1621 gcgcaggccg aacagcgttg ggctatgctg tgaagtcggc gctcgcgtag gggcggcgcc
     1681 gaggagactt cgtgagcgac acgagcactc gtcgcgatcg gccgactctt ccaacctcgg
     1741 gcgttggaag ggaagagatc cggtcgtcgt tgttaaagcc ttttttattg aggttgcgtg
     1801 acgagcgcgg cgacgccgcg gtgcgcgccc tgctcgcgag cgtcggcatc ccgccgagcg
     1861 cggtggacag cgacaccggc tggctcagcg tagccgcgac ccggcgcatc atgagcgcgc
     1921 tctcgacggc gctcgggcgc ccagcgctcg agacgcgcgg ccagtggatg acccacccgg
     1981 aagtgctcgg ttcgtacgtg cgcatgttgc gcgtcgcgag ctcgcccgag gacgcttacc
     2041 gatatctctc ggccaacgcc gccgaaacca cccgcgtcgg cacgtacgag ctcgggacga
     2101 tcagcgtcaa ccacgccgag ctcagctacc accgccgcgc cgaagccgag tcggagcaaa
     2161 acgacccgct gctctgcgtc gcccgacagg ccgagctcgt gagcgtcccc cgcatttggg
     2221 gactctctga cgcgcacgtg gctcacccgc gctgcctcgc cgaaggtgcc gacgagtgtt
     2281 tgtacatcct cgactggagc gcacccaacc gcaagcgctg ggtgatcctc ggctgcctgc
     2341 tcggagggct catctgtggc ggcgcggtcg cgatctcggg gagcttcatc gcgtcaatca
     2401 tcgcggcgtt gatcgggttt ggttttgggg gggcgctctc gtcgctctct gcgcgaatcc
     2461 gtgaagaacg cgccgcgcgt gccttcgaga agaatcgcat cgccgcgctc gaacgcggct
     2521 tggaagcgca cggccacttc cgcgacgccg ccggcgatct cgctggcgtc acgctgggcg
     2581 gcaagtaccg gatcctccgt aaaatcggct cgggtggcat cggcgcggtg tacgcggcgg
     2641 agcacatcgc gctcggctcg cgtgtcgcgg tcaaggtgct gcgcggcgcg gccgcggtcg
     2701 acgcctccga gatcgcgcgc ctccgccgcg aagcgcgcgt gcaagtctcg atcgagcatc
     2761 cgaacgtcgt acgcgtgctc gacctcgatc agctacccga cggctccatc tacgtcgtga
     2821 tggagcagct ccaaggccga agcctcgccg cgcgcttgaa gtcgggcggc ccgctctcgg
     2881 cgaccgaagc cgtcacgatc ttccgcgccg tctgcaaagg gctttccgcc gcgcaccagc
     2941 tcggcgtcgt tcaccgcgac ctcaagcccg gcaacatctt cttgtgcgac gatcgcagcg
     3001 tgaaaatcct cgacttcggc atgagcaaat tcgcagaagc cgagtcactc acccaagatg
     3061 gctacacgct cggcacgccc gagtacatgt cgccggagca gtgcatcggc gctcccgtcg
     3121 acccacgcac ggatctctac gccttcggcg tcctgatgta cgaagcgctc gtcggcagct
     3181 tgcccatttc gggcaagaac cgccgtgaat tcctcgagct ccaccaacgc atgatccccc
     3241 cgtcgatgcg cgagcgccgt cccgacctcg acatccctgc cgagctcgac gaggccgtca
     3301 tgcgctgctt gaaaaagcgc gcatccgagc gccccgccac cgctcgcgag ctcgacaaac
     3361 tcctcagcaa agttccgatc gccggcgtca gctccgtcga cagccagcct ccgacgccga
     3421 gccgcgcgct tcgctgaact aggagcgctg caccagcgaa gcattcatcg caggcccgcg
     3481 gtcaatcgac ggcgcccgat gcttgcgcat tggagtccga accaagttcg actcctcggt
     3541 atatctcgtc gagaggaaaa cgacacgccg ggtcgttcaa gacgacttcc tcgccagcgt
     3601 tcacgtcgtg ctcagcccat cccgcggggg tgcgctcgac aatcgtgatt tgcgcgcgcc
     3661 ggtgagagac gaatatcacc gccttgagac tcgacagatg cttgtagtgc cggagcttct
     3721 caccgcgatc gtagtcctcg gtcgaggggc tagttacttc taccaacagc gttgggttga
     3781 tcaccgcgtt ctcatcgata gccgagacag cccgaggccc gcaaactacg accgcatcgg
     3841 gaaaggtcga caaatcagtt tcttcgatcc gcaccttcag gtccgaagta gggacgcgac
     3901 acccctggcc caggacgcga accagggtcg caatcgcggc agcgcttaag tccgcgtgag
     3961 cgggcgtacc gccagccatc gcgtaaattt cgccgtccag atattcgagc ttgatcgcgc
     4021 tatccttcaa tgcgcgaagg taggccgcgt acgaatagtg aaggcgccgc gctgtcgaca
     4081 tttccggaac ttagcatcaa tcacgctgcc cctggcacca atcacggtgc ctgcgggata
     4141 cgacgccgca gcggttgtaa cgtccgcgct cgccacgctg cctcgatggt gcttggctag
     4201 gcgcttggga ggaaccgtga cgcgcaactt gctgcaccta gcgacctgca cgctcggcgc
     4261 gattgccatg gcatgcagcg ccgccgacgc atgccctgac gagcaaccga ctcgaagcgt
     4321 agagaccgga acgacggacc aactcgcgtt ggtctacgag tccgctccgt ggagtggtcc
     4381 actcgagccg ttccccgcga tgggcagctt gtcattcaaa cacgacctcg ggatgactcc
     4441 gaaaatcgtc gctacttacc tttcgttcag cgaaagtggc accggctcgt cgagcgtcgc
     4501 cgaaagcgct ggtaacgccg cgttgatcga ctgcgtcgac tcgcaggtga tccgcgttcg
     4561 caacgatacc tgcgaaaagc actttttcat tcgagtggtc gcgatggcaa ctccgggagc
     4621 acccaccgac gtgacctgct cggaggacta gggggtgtcc caagttcccc gccctgctac
     4681 tagcgactct ctccgcgttc acgcaagatc cgcgcggctt ctctatacac cgcgcgttgc
     4741 ggcattgcct cggccaaacg ctcgtagacc tgcgcggcgt ccgcgaatcg cccttcgacg
     4801 acggcgtcac tcgcagcgcg ctccaagctg atgcccgttt catgcggggt aagcttggag
     4861 gtcggcggcg tgggctcgcg gcgtcgcgca gcgggtgcgg cgggcttcga aagcgacttg
     4921 gcagcccgtg caggttgaac gttgctctga gaagcagccg gggttccgcg agcggctccc
     4981 atcacattgg ctgcaatggc catgctcgac atcaacgcgc cgaccaacgc tgccacgagg
     5041 atgcgtcgct tgggcggaat gctgcgcttc cgaggcgccg gtgtggcctg actaggtggc
     5101 gccggaggtg cattgctctc gtccgccgcg ctggctgggg ccggagctcg gcgcgccagc
     5161 agatgctcga cgggaaccaa ggtggttctg agattcgcgc gagaggtacc cgtagcaagg
     5221 tccggcaagg acgccacggc tggctcgcgc gcagcaaagc cttcgcgcat ggggatgagc
     5281 cgcgtcttcg gcaacgagtc cggcgtcgcg acggcgcctg ctccacccgc gttcggctcc
     5341 cccggcgcgg cgcgcggcag ctcacgaatc gaaatgattc tggtgtttgc cattggtgtt
     5401 ctcctgttca cgggctttcg agtacacgct ttcggggagc tcgagcccac gctctcgcaa
     5461 gtgcggcagg aagctcggca gcatgccggt gggggccaat tcgctcagca cgcgaccgtc
     5521 ggcggcgaaa cccaggtagt tccgtgcgaa gagatcacgc agcttgtatc gattcgactc
     5581 gaggtcgaag ccgagcactt ccgtgatgtg gctcacggcg cggctgccgt cctgcatccg
     5641 cgtcacttgc acgatgacgt tcacgcccga agcaatctgc atccgcagcg cggtgagagg
     5701 caggttcacg tcgctcatca tcgccatggt ttctagacgg gttagcgtgt cgcgcggata
     5761 ggtagcgtgc agagtgccca tgcagccgcc gtgcccgctc accatggctt gcacgatgtc
     5821 gagcgcctcc gcgccgcgaa tttcaccgat gatgatgcga tcgggccgca tgcgcagcgt
     5881 cgcgcggaag aggtcgcgga tgctcactgc gccgcgccca tgctcgtcgg cggggcgcgc
     5941 ctccatcgaa acgacgtggg gccgttgcac ctggacctct ttcgaatcct cgatcacgac
     6001 cacgcggtca cgctcgggcg caaagcttgt caacacgttc aagagcgaag tcttcccgct
     6061 gccggttccg cccgcgatca ccatgttcag tttggcctcg accaaggcgg ccagggtctg
     6121 agcggcgtct tcgcacaagc tgcccgagcg caacagatgt c
//
LOCUS       DASTKS010001350         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_222558, whole genome shotgun sequence.
ACCESSION   DASTKS010001350 DASTKS010000000
VERSION     DASTKS010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222558"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1300
                     /locus_tag="VFQ35_29015"
     CDS             326..1300
                     /locus_tag="VFQ35_29015"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9934794.1"
                     /translation="MRVSFSAFIVSALLVCSCGREKKSESSAGAGSASAPQKKGFVIG
                     VSQCNLGEPWRVQMNADIAQAASAHPNLKLLLKDAQNDSLRQRAQVEELLEQKIDVLI
                     ISPKESAPLTKPVAEVYKKGIPVIVLDRAVEGDDFTSFIGADNRRIGREAGKFIAATL
                     GGKGNVVELKGLMTSSPGRDRHEGFVEGLAEAKASGVKVVFEADMQWLEPNARKEMES
                     ALAANKAIDLVYAHNDPGAHGAYLAAKEAGRERQMKFVGIDALPHEGVRYVREGILDA
                     TLAYPTGGAEAIALASKLLAGEKVPKKVTLGTRLYTKENVERGGQAIE"
     gene            1359..>2433
                     /locus_tag="VFQ35_29020"
     CDS             1359..>2433
                     /locus_tag="VFQ35_29020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934795.1"
                     /translation="MSKKVYVYWPGDARQKPNELALPNMEAATQQMERALKKLGRNPE
                     RIPGFLSKPHEALDKLGPIDEPLVGIFVHWVYAPHTTDGVVGKDNPLLLASNFSGQWP
                     GLVGLLNTGASLESLNRKHSRIFTTAEDWSQDAVFMERLDEWCSSGRIRYSEQGIRYH
                     AEVSAEATHRAQRVAAGIRRRRPLLLMFGDTSMGMINGYFGPRLLSRYGFAEHKIDQA
                     WIIDRGSRVVDRRINDAYSFVKDRGVVFHYGETGAQDFTEQATREQLRDYLAILDMAG
                     EFGADCLGIQYQLGLIPLRPPTDFAEGLFNSSCRPESNGDTIACATEADQGNALPMEM
                     LKRLLKERGLHQAVMFHDVRWGAE"
BASE COUNT          449 a          765 c          815 g          404 t
ORIGIN      
        1 cgaggtcgcg acgacacgga gctctttcaa cggcgcgagg cgccgcggcg aacgccgggg
       61 ggctcgcgac gcttctcact cgagatcggg tgttcttgcc gcgccgagcg ttggttccca
      121 cgcggttgtg acgtgttgac agattttgtc acaatgtctc gcgccgctcg ggaacgtcag
      181 agtcacgccg ctgcagtcgc cgcgctgccg ttgctgaacg cagcagcatc acccctggag
      241 cgcaacgacg gagccggact ggcgcagtcc tcgtctcgtg cccctagtcc tagtgtcgcg
      301 cgcggcgctc ccatatagtg ggcccatgcg ggtctcattc agcgcgttca tcgtctcggc
      361 gctcctcgtg tgttcctgcg gacgcgagaa gaagagcgag agctcggccg gcgcgggatc
      421 ggcctcggcg cctcaaaaga aggggttcgt catcggcgtc agtcagtgca acttggggga
      481 gccctggcgc gtgcagatga acgcggacat cgcgcaggcc gcatccgcgc acccgaactt
      541 gaagctcttg ctgaaggatg cgcagaacga ttcgcttcgt cagcgcgctc aggtcgaaga
      601 gctactcgag cagaagatcg acgtcttgat catctctccc aaggagtcgg cgcccctcac
      661 gaagccggtc gccgaagtgt acaaaaaggg gattccggtg atcgtgctcg atcgcgccgt
      721 cgaaggcgac gacttcacga gcttcatcgg cgcggacaac cgccgcatcg gtcgcgaagc
      781 ggggaagttc attgccgcga cgctgggcgg caaaggcaac gtcgtggagc tgaaagggct
      841 gatgacgtct agccccgggc gcgaccgcca cgaagggttc gtagaaggtt tggccgaggc
      901 gaaagcctct ggcgtgaagg tcgtgttcga agccgacatg caatggctcg agcccaacgc
      961 ccgcaaagag atggagtcgg cgctcgccgc aaacaaggcg atagacctcg tttacgcgca
     1021 caacgacccg ggtgcgcacg gcgcctacct cgccgccaag gaggcgggtc gcgagcgcca
     1081 gatgaagttc gtcggaatcg atgcgctccc tcacgagggt gttcgctacg tacgagaagg
     1141 catcctggac gccacgctcg cgtacccgac cggcggcgcc gaggcgatcg ccctcgcgtc
     1201 gaagctcctc gcgggcgaga aggtgccgaa gaaggtgact ctcggaacgc gcctctacac
     1261 gaaggaaaac gtggagcgcg gcgggcaagc catcgagtga gcgcgcttgg ctcggcgcgc
     1321 tgggggcagt atcgtcccgc cctcgaggag agccgccgat gtccaagaag gtttacgtgt
     1381 attggcccgg agacgcccgc cagaagccga acgagctcgc gttacccaac atggaggccg
     1441 cgacgcagca gatggagcgc gctctcaaaa agctcgggcg caacccggag cgcattccag
     1501 ggttcctgag caagccgcac gaagcgctgg acaagctcgg ccccatcgac gagccgctcg
     1561 ttggcatttt cgtgcattgg gtctacgcgc cgcacacgac cgacggcgtc gtgggcaaag
     1621 acaatccgct gctactcgcc agcaacttct ccgggcaatg gccgggcttg gtggggttgc
     1681 tcaacaccgg cgcgagcctc gagagcctga accgcaagca ctctcgcatc ttcaccaccg
     1741 ccgaggactg gtcacaggac gccgtgttca tggagcggct cgacgagtgg tgctcttcgg
     1801 ggcgcattcg atacagtgag cagggcattc gctaccacgc cgaggtgtcg gcggaggcga
     1861 cgcaccgcgc acagcgcgtc gcggcgggca ttcggcggcg tcgcccgctg ttgctgatgt
     1921 tcggcgacac ctcgatgggc atgatcaacg gctatttcgg cccgcggctg ctctctcgct
     1981 acggtttcgc cgaacacaag atcgatcagg cgtggatcat cgatcgtggg tcgcgcgtgg
     2041 tcgaccgtcg gatcaacgat gcctattcgt tcgtgaagga ccgcggcgtc gtgttccact
     2101 acggcgagac cggggcgcaa gacttcaccg agcaagccac gcgtgagcag ctgcgcgact
     2161 acctcgccat tctggatatg gcgggcgagt tcggtgcgga ctgtctcggg attcagtatc
     2221 agctcgggct catcccgctg cgcccgccga ccgacttcgc ggagggcttg ttcaactctt
     2281 cgtgccgccc ggagtcgaac ggcgacacga tcgcgtgcgc caccgaagcc gaccagggca
     2341 acgcgctccc gatggaaatg ctgaagcgcc tcttgaaaga gagggggctg catcaagcgg
     2401 tgatgttcca cgacgtgcgt tggggcgccg agc
//
LOCUS       DASTKS010001351         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_668192, whole genome shotgun sequence.
ACCESSION   DASTKS010001351 DASTKS010000000
VERSION     DASTKS010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668192"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2291)
                     /locus_tag="VFQ35_29025"
     CDS             complement(<1..>2291)
                     /locus_tag="VFQ35_29025"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9934796.1"
                     /translation="ARQFVQSGASATLQVLLADKAFSETRAAAPYLAYVAARPQRAEV
                     PLVSSEAAAGIRAWLIDWLAGELSVSPSEVDVRRSFQSYGLDSVRLIALTGDLASFSA
                     RRIAATAAYDYPNVELLAEHAIAAPGLLAVPPLSRPGPLPLGARQLRLVALERAHPGE
                     IIDHLTEVFTLEPVSEATVRSVLERLVERHEALRCHIIEGTAGPAQFAERVLEPFPLH
                     VRDVPTDSGSFDEEVRRVALAQDRAPFELNGPLVRAALVRGAAGDRALVLTLHHLVGD
                     GWSMALLRRDFTSLMKSALGQSADPLPTLGRQQADVAARERAFLQSTEASASLAFWSA
                     RLGDHPRLRATLPGTTFHGAVERRSLPKETMERLARVAHRLGVTPYIALLTAFCRVLG
                     RESGEDDVLVNTHLFNRSDEDERSLCGYLVNVLTVRTALTSAMPFSEAARSVHVAWME
                     ALEHALPIDEVVRTLRPEAYAERYMPSASAFNMLPALPEGAASGSGPVAPRPDLAPPP
                     GFMFFERMLLVAPDPSGNQWLTLWFNEATVDPPAAAKFLEVLVLDLDAISADNPPAST
                     RVSVCDRGDVVRGAARRFVSERLGVEAWAVSDDVALTRLGLDSVLAAELASALEDALG
                     SPVDASVVFRHRSIDGIARALSVEPVPAKRVPSGEPGPLSCTELGLFQLFTRDPGPQD
                     PYNLLLTLRIDAPPDAVRLRQALSLLGARHEALRTGFYVGNDGVPRRRVLESVEPPFA
                     VVRVSPAELATARRAQGDALRRV"
BASE COUNT          432 a          756 c          765 g          338 t
ORIGIN      
        1 gaacgcgacg aagcgcgtcg ccttgcgcgc ggcgtgcggt agcgagttcg gccggtgata
       61 cccgcaccac ggcgaacggc ggctctacgc tctcgagcac gcgtcggcgt ggaactccat
      121 cattgccgac gtagaaaccg gtacgcaacg cctcatgacg agcgccgagc aacgagagcg
      181 cttgcctcaa ccgaacggcg tctgggggcg cgtcgatccg cagagtcagc agcaggttgt
      241 acggatcctg cgggcctggg tctctcgtga atagctgaaa caagccgagc tcggtgcaag
      301 agagaggccc tggttcgccg ctcggaacgc gcttggcggg gactggttcg acggagagag
      361 cccgcgcgat gccgtcgatc gagcgatgcc gaaacaccac gctcgcatct acgggcgagc
      421 cgagggcatc ttccagcgcg ctggcgagct cggccgcgag caccgagtcc aaaccgagcc
      481 gagtgagcgc gacgtcatca cttacggccc acgcctccac cccgaggcgc tcagacacga
      541 agcgccgggc cgccccgcga acgacgtcgc cgcggtcgca cactgacaca cgagtcgatg
      601 ccggggggtt gtccgccgag atcgcgtcca ggtcgagcac gagtacttcg aggaacttcg
      661 cggcggcggg cggatccacg gtcgcttcgt tgaaccaaag cgtcagccac tgattgccag
      721 acggatccgg cgcaaccaac agcatccgct cgaagaacat gaaaccgggc ggcggcgcga
      781 gatccggacg gggcgcgacg gggccgctcc ctgaggcggc tccttcggga agcgcgggga
      841 gcatgttgaa cgcggacgcg gacggcatgt agcgctctgc gtaagcttcc ggacgcagcg
      901 ttcgcaccac ttcgtcaatc ggaagggcgt gctcgagagc ctccatccac gcgacgtgga
      961 cgcttcgcgc cgcctctgaa aacggcatcg cgctcgtcaa cgccgttcgc accgtaagta
     1021 cgttgacgag gtagccgcaa agcgaccgct cgtcctcgtc cgagcgattg aagaggtggg
     1081 tgttgacgag cacgtcgtct tcgccgctct ctcgtcctag gaccctgcag aaagcagtca
     1141 acaacgcgat gtacggggtc acgccgagcc ggtgagcgac cctcgcgagc cgctccatcg
     1201 tctctttcgg caggctgcga cgctcgacag ccccgtgaaa ggtcgtgccc ggtagcgtcg
     1261 cacgcaggcg aggatggtcg cccaggcgcg ccgaccagaa ggcgagcgac gcgctcgctt
     1321 cggtactttg caaaaacgct cgctcgcgcg cggcaacgtc cgcttgttgg cgcccgagcg
     1381 taggcaatgg atccgcgctc tgcccgaggg cgctcttcat gagagaagtg aagtcgcgcc
     1441 gcaagagcgc catcgaccag ccatcgccga ccagatgatg tagcgtcagc accaatgctc
     1501 ggtctcccgc cgcgccgcgc accaaagctg cgcgcactag cggcccgttc agctcgaacg
     1561 gcgcgcgatc ttgagccagc gcgactcttc tgacttcctc gtcgaacgac ccggaatcag
     1621 tggggacgtc gcgcacgtgc aaagggaacg gttcgagcac ccgctctgcg aactgcgcgg
     1681 gcccggcagt gccttcgatg atatggcaac ggagcgcctc gtgacgttcg acgaggcgct
     1741 cgagcacgct acgaacggtc gcctctgaca ccggctcgag cgtaaagacc tcggtgaggt
     1801 gatcgattat ctcgccggga tgagcgcgct ccaaagccac tagccgcagc tgccgagcac
     1861 cgagcggcag tgggcccggc cgtgatagcg gaggtacggc caggagcccg ggcgcagcga
     1921 tcgcgtgctc ggccaagagc tctacattcg ggtaatcgta agcggcggtc gcggcgatgc
     1981 gtcgggcact gaaagacgca aggtcgccgg tcaacgcgat gagccgaacc gagtcgaggc
     2041 cgtagctttg gaaactgcgg cgcacgtcga cttcgctcgg cgaaacggaa agttcccccg
     2101 cgagccagtc gatgagccaa gcgcgaatgc ccgcggctgc ctcactcgaa acgagcggca
     2161 cctccgctcg ctggggtcgc gcggctacat aggccaagta gggcgccgca gctcgagtct
     2221 cggagaatgc tttgtccgcg agcagcacct gcagcgtcgc gcttgcaccg ctctggacga
     2281 attgccgcgc g
//
LOCUS       DASTKS010001352         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_668226, whole genome shotgun sequence.
ACCESSION   DASTKS010001352 DASTKS010000000
VERSION     DASTKS010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668226"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFQ35_29030"
     CDS             complement(<1..603)
                     /locus_tag="VFQ35_29030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934797.1"
                     /translation="MRKPALLRSFIALLAAGCGSEAHHPVEAEEPVPTGPISMGQGLR
                     IFSGRAAVLTDGNACTTEKGATGDRWCGFVALDANDSRSLYVVNVSQLLAGAPVSCDV
                     PDPNCLLLTSRVGSSAANFHPTGFDGDTLVYYDETVSPYVWRPGMERGRLLMSRPDGV
                     DVIFCSPAPVGTAVGCLAVPRDQFSAVVLADIYAGDADGET"
     gene            complement(778..1221)
                     /locus_tag="VFQ35_29035"
     CDS             complement(778..1221)
                     /locus_tag="VFQ35_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9934798.1"
                     /translation="MSTLDIAEVAKRARLPASTLRFYEEKGLIESVGRRGLRRLYEES
                     VLERLALIGLGRAAGFSLEEIAGLLRDGTRIDRSALAAKADELDCAIQKLTKMRDGLR
                     HAAACRAPSHMECPSFRRLLRAAASGLIHDGPSSAPVRRRRNKHS"
     gene            1295..2143
                     /locus_tag="VFQ35_29040"
     CDS             1295..2143
                     /locus_tag="VFQ35_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9934799.1"
                     /translation="MTTQTAPNDQQSLWNGPAGKAWGESQALMDSILRPFQDLLLDVL
                     PSDARAVLDVGCGTGSTTLAVAERLGASGSTTGIDISEPLLTVARARAAQLGQPIDFV
                     LADAQTHPFGGARFDTVISRFGVMFFENPTHAFGNLRRATLPGGHLRFVAWRSADENP
                     FMTTAERAAAPLLPSLPVRRPDEPGQFAFADRARVDGILTDSGWTQVEIRPIDVTCAM
                     PTSDLIPYVTRFGPVGRLLEESSGDIRARFTERIGAAFAHYTHGSEVRFTAACWLVGA
                     RAERVE"
     gene            2180..>3822
                     /locus_tag="VFQ35_29045"
     CDS             2180..>3822
                     /locus_tag="VFQ35_29045"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9934800.1"
                     /translation="MESGGRDASDLGEPSALFGGDGGVATAARSLDWSRRALGPVEGW
                     PPLLRSFVQLALDSPHAAVVLWGRDALVFANDRAARMFSEDLGEVLGRSWFDFSTRGS
                     DDARALYARVLNGETVHERGRNLAVVDRGRTEEAWFDLEYTPIRGADGRVAGVLHLAR
                     ERTDEFMTSRQHAFLARLSDALAPLVEPFQVEEVATRLLGAELGVARVMYGVVASDDE
                     HIHFARSYVAPGVPEILGSFRMTDFGQRAVAALRAGKSLVSDSIDSDEALTPTERDAY
                     LRLGIQALAGVPLVKDSRLVANISVHDVKPRRWTRHELELIAETAQRTWSAVERARAE
                     AALRASEERYRSLFETMQEGYVLYELVRDESGVVVDARFLEINPAFAAVTGVPRERCI
                     GRLRSEVLPRPTELAPPALVRALERGESFRFEAYAPTVGRWYDIRVHPESHDRIVVLY
                     DDVTARKQAEHTLRETDARNAYLLELSDRLRAASDPIEIQDVATALLAERLNAVRCYY
                     SEFDFARGTAEVRTESVRGNAASVVGTYRLTDFHGHIAAL"
BASE COUNT          661 a         1283 c         1221 g          657 t
ORIGIN      
        1 ggtctcaccg tccgcatctc cggcgtagat gtccgccagg acgacagcgg aaaactgatc
       61 ccgtggcacc gccaaacaac cgaccgccgt tccaaccgga gcggggctac agaagatcac
      121 gtcgactcca tcggggcgcg acatcaagag ccgtccgcgc tccatccccg gtcgccacac
      181 gtacggcgaa accgtctcgt cgtaatagac caaagtatcg ccgtcgaagc cggtgggatg
      241 gaaattcgcg gcgctgctgc cgacgcgtga agtgagtagt aagcagttcg gatcggggac
      301 atcacagctc accggcgcgc ccgcgagcaa ctgcgacacg ttcaccacgt acaggctgcg
      361 gctgtcgttg gcgtcgagcg caacgaatcc gcaccaacgg tcgccggtcg cgcctttttc
      421 cgtggtgcac gcgttgccat cggtgagcac cgcggctcta cccgagaaga tgcgcaaccc
      481 ctgccccatc gaaatcgggc ctgtcgggac gggctcctcc gcttcgaccg gatgatgcgc
      541 ttcactgccg cagcccgctg ccagcaacgc gatgaacgaa cggagaaggg cgggtttacg
      601 catcgaatgt gaccccaagc gtgccgccga tttcaaaccg gagctcgcgt aggcgcaagc
      661 ttttcctcgg ctcactcccg ctttttcggc gactcgaacc gctcttccga gcccgatgaa
      721 tttctcgagc gcgggtgcca ggactcgaaa cgaggcaggt gcgcgtccga aatcccctca
      781 cgagtgcttg ttgcggcgtc ggcgcacggg tgcgctcgac gggccgtcgt gaatcagccc
      841 gctcgcggcc gcgcgcagga gccgacgaaa gctggggcat tccatgtggc tgggcgcgcg
      901 acaggcagcc gcgtgacgga ggccgtcacg catcttggta agcttctgga tagcgcagtc
      961 gagctcgtcg gccttcgccg ccaatgcact ccggtcgatg cgagtgccat ccctcagcag
     1021 gcccgcgatc tcctcgagcg agaaccccgc cgcccgtccg agtccgatca acgcgagcct
     1081 ctcgaggacc gattcttcgt acaagcggcg taagccccgc ctcccgacgg actcgatcag
     1141 ccccttctct tcgtaaaagc gcaacgtcga agcgggcagg cgagcgcgtt tggcgacctc
     1201 ggcgatgtcc agcgtgctca tgggcttgac ctcaagtcga cttgaagttc catagtgatg
     1261 gcgtgaccct tcatccgtca aggaggactc ccacgtgacc acgcaaactg caccgaacga
     1321 ccagcagtcg ctttggaatg gcccggccgg caaagcttgg ggcgagtcgc aagcgctaat
     1381 ggactcgatc ctccggccct ttcaagacct gctcctcgac gtgcttccca gcgacgcgcg
     1441 cgcggtcctc gacgtcggct gtgggacggg gagcaccacg ctggcagtcg cggaacgact
     1501 cggcgcttcg ggctcgacca ccggcatcga catttcggag ccgctgctca cggtcgcgcg
     1561 ggctcgggct gcgcaactgg gtcagccaat cgacttcgtg ctcgctgacg cgcaaacgca
     1621 ccctttcggc ggcgcgcgct tcgacaccgt catctcacgc ttcggcgtga tgttcttcga
     1681 aaaccccacc cacgcgttcg gcaacctgcg tcgggcgacg ttgcctggcg gacatcttcg
     1741 cttcgtcgct tggcgaagcg ccgacgagaa ccctttcatg acgacggccg agcgcgcggc
     1801 cgcaccgctc ttgccgagcc tgccggttcg acgaccagac gagccgggac agttcgcgtt
     1861 cgccgaccgc gctcgcgtcg acggaattct gaccgatagc ggatggaccc aagtggaaat
     1921 tcggccaatc gacgtgacgt gcgcgatgcc aacgagcgac ctgataccct atgtcacccg
     1981 tttcggcccc gtggggcgcc tgctagaaga gtcgagcgga gacattcggg cccggttcac
     2041 cgagcggatc ggagccgcct ttgcccacta cacgcatggc tcggaggtac gatttaccgc
     2101 tgcctgctgg ctcgtgggag cccgggccga acgagtcgag tagccaacga cgggagggct
     2161 gatctacctt cgagttcgca tggaatcagg ggggcgagac gcatcggacc tcggcgagcc
     2221 ttccgcgctg tttggcggcg atggaggcgt ggccactgcg gcgcgttcac tcgattggtc
     2281 ccggcgcgcg cttggtccgg tcgagggctg gccgccgctg ctcaggagct tcgtgcagct
     2341 cgctctcgac tcacctcacg ccgcggtcgt gctttggggg cgagacgcgc tcgtgttcgc
     2401 gaacgaccgc gcggcacgga tgttttcgga ggacctcggc gaagtgctcg gccgctcttg
     2461 gttcgatttc tcgaccaggg gttccgacga cgcccgcgct ctttacgcgc gtgtcctgaa
     2521 cggcgagacc gttcacgaac gcggccgcaa cctcgcagtc gtcgaccgag ggcgaaccga
     2581 agaagcctgg ttcgacctcg aatacacgcc gattcgcggc gccgacggcc gcgtcgccgg
     2641 ggtcttgcac ctcgcccgcg agcgcaccga cgaattcatg acgagtcggc agcatgcgtt
     2701 tctcgcgcgg ctgagcgatg cccttgcccc cctcgtcgag ccatttcagg tggaggaggt
     2761 agcgacgcga ttgctcggcg ccgaactcgg cgtggcccgc gtgatgtacg gggtcgtggc
     2821 cagtgacgat gagcacattc acttcgcgag gagctacgtc gcaccgggag tgcccgagat
     2881 cctgggtagc ttcaggatga cggacttcgg ccaacgcgcc gtagcggcgc tccgcgccgg
     2941 aaagagtctg gtcagcgatt cgatcgatag cgacgaggcg ctcacgccga cggaacgtga
     3001 cgcctatcta aggctcggga ttcaggcgct cgcaggcgta cccctggtca aggactcgcg
     3061 cctcgttgcg aacatctccg tccacgacgt gaaacccagg cgctggaccc ggcacgagct
     3121 cgagctcatt gctgaaacgg cccaacgcac gtggtccgcc gtggaacgtg ctcgagccga
     3181 ggctgcgctg cgcgcgagcg aagagcgcta ccgttcgctg ttcgagacga tgcaagaggg
     3241 ttacgtgctc tacgagctcg tccgcgacga aagtggggtc gtggtcgatg ctcgctttct
     3301 cgaaatcaat cccgccttcg ccgcggtcac gggtgtgccg cgagaacgct gtatcggacg
     3361 actgcgaagc gaggtgctgc cgcgccccac cgaactggcc ccgcccgccc tcgtccgcgc
     3421 gctcgagaga ggggaatcgt tccgcttcga agcctacgca cctacggtcg ggcgctggta
     3481 tgacatccgc gtccacccgg agagccacga tcgcatcgtc gtgctctacg acgacgtgac
     3541 ggcgcgcaag caagccgagc atacgctgcg ggagaccgac gcgcgcaatg cctacttgct
     3601 cgagctctcc gaccggctcc gcgcggcttc ggatcccatc gagatccagg atgtcgccac
     3661 cgcgctcctc gccgaacggt tgaacgcggt tcgttgctac tattccgagt tcgatttcgc
     3721 tcgaggcacc gcggaggttc gaaccgagag cgtgcgcggc aacgccgcga gcgtggtcgg
     3781 gacgtatcga ctcaccgact ttcacggtca cattgccgcg ct
//
LOCUS       DASTKS010001353         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_222784, whole genome shotgun sequence.
ACCESSION   DASTKS010001353 DASTKS010000000
VERSION     DASTKS010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222784"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFQ35_29050"
     CDS             <1..632
                     /locus_tag="VFQ35_29050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9934801.1"
                     /translation="IITRDPSAYLLLAPALFGPAAYIEQLRKRRVRAIDGQIDGFTLS
                     LANALKATPSIGNALGYVQPLLPEPLAQEVGLALKEMKLGHTVEQVLLSMGVRIRSVQ
                     LDAVLSSLLIGRQVGGDVGKILEKTAATLREMARLAGVVRTKTAEGKAQLSVLAAFPV
                     LIVFMFDTVSAGYFDPLTHSVVGWVIIVSAVVLWIASLLIARKVLEVDL"
     gene            629..1534
                     /locus_tag="VFQ35_29055"
     CDS             629..1534
                     /locus_tag="VFQ35_29055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9934802.1"
                     /translation="MSGFVFKSSALALSFAALSLFVYTLVSAPLAPANHLGVRGLKRT
                     RTQQLNPVFREIEPVLRWLGVRLRPFLSDALHARIDRQITLAGDFWGLLPEECCALYV
                     LSTFGGLGLGVAYAAMLGRGGLYMVLGVVVGLMLPYLQLSGIQQERRKQIQNGLPYVI
                     DLLSLGLSAGLDFPGALRQVVEKSSDTKGALVEEIQLVLQELQVGKTRRQALDQFAQR
                     NPGESVREFVGAVVQADIRGNPLGPVLEIQAEVSRQHRSVRAEESAARASVKMLLPML
                     LVFGAILLLIAAPMAMQVNGTFKGD"
     gene            1538..>2079
                     /locus_tag="VFQ35_29060"
     CDS             1538..>2079
                     /locus_tag="VFQ35_29060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934803.1"
                     /translation="MNEIASLEFQLRFADGRRQTLTVDSPSALLGSGAHCEVRLPPED
                     AAAEELQVTLTPGGLIGSARRLDRAILLDGAPFVEGRILPGSELTIGGTTVTSRQCEA
                     ASHATRSRPKSDPSSQGIRVFAALGLPLAGYMLLTAERHSAKFPAVVEAPPLWSAPAE
                     ACPEREREASEAFGQAEVLRA"
BASE COUNT          333 a          670 c          684 g          392 t
ORIGIN      
        1 gcatcatcac gagagacccc agcgcttacc tcttgttggc gcccgcgctc ttcggccccg
       61 ctgcgtacat cgagcagctg cgaaagcgcc gcgttcgggc catcgacggg cagatcgacg
      121 gctttacgct ctcgctcgcc aacgcgctca aagcgacacc gagcatcggg aacgcgctcg
      181 gctacgttca gccgctcttg ccggagccgc tcgcgcaaga ggtgggactg gccttgaagg
      241 agatgaagct aggccacacc gtggagcagg tgctcttgag catgggcgtt cgcattcgca
      301 gcgtgcagct cgacgcggta ctctcgtcgc tgctcatcgg gcgccaagtc ggcggggacg
      361 tcggcaagat cctggaaaag accgcagcga cgctgcgcga gatggctcgg ttggcgggcg
      421 tggtgcggac caagacggca gagggcaagg cgcagctcag cgtgctcgcc gcgttcccgg
      481 tgctcatcgt tttcatgttc gacacggtga gcgccggcta tttcgacccg ctcacgcatt
      541 cggtagttgg ttgggtgatc atcgtgagcg ccgtggtcct gtggatcgcc tcgctgctca
      601 tcgcccgcaa ggtcctggag gtcgacctat gagcggcttc gtattcaaga gctcagcgct
      661 cgcgctgagc ttcgcggcgc tttcgctctt cgtctacacg ctggtgagcg caccgctcgc
      721 tcccgcgaac cacctgggcg tgcgcggctt gaagcggacg cgcactcaac agctgaatcc
      781 ggtgttccga gagatcgaac ccgtactgcg ctggctcggc gtgcggctcc gtccgttcct
      841 ctccgacgcg ctccacgcgc gcatcgatcg acaaatcacc ctggctggag acttttgggg
      901 gctcttgccc gaggagtgtt gcgcacttta cgtgctctcg actttcggcg ggctgggcct
      961 cggagtcgcc tacgctgcga tgctggggcg tggagggctc tacatggtac tcggcgtcgt
     1021 ggtcggcctg atgctgcctt atctgcagtt gtctgggatc caacaagagc gacggaagca
     1081 gattcaaaat ggcttgccat acgtgattga cctcctgtcg ctcgggctct ctgccgggct
     1141 cgattttccg ggtgcgctac gtcaagtcgt cgagaagagc agcgacacca agggcgcgct
     1201 cgtcgaagag attcagctcg tcttgcagga gctccaagtc ggaaagactc ggcgccaagc
     1261 gctcgatcaa ttcgcgcaac gaaaccccgg cgagtccgtg cgcgagttcg tgggggcggt
     1321 ggtgcaagcg gacattcgcg gcaatccgct cggtccggtc ctggagatcc aagccgaggt
     1381 ttcgcggcag caccgctccg tacgcgccga ggagtccgcc gcgcgcgcga gcgtaaagat
     1441 gttgctgccg atgctcctcg tgttcggcgc cattttgctg ctcatcgctg cgcccatggc
     1501 gatgcaggtc aacggcacct tcaaagggga ctgaaacgtg aacgaaatcg cttcgctcga
     1561 gttccagctc cggttcgcag acggtcggcg tcaaaccctg accgtcgact ctcccagcgc
     1621 tctgctcggc agcggcgctc attgcgaagt gcggctgcca ccggaagacg cggccgcaga
     1681 agagcttcaa gtcacgctga ccccgggcgg gctcatcggc agcgcgcggc gcttggatcg
     1741 cgccattttg ctcgacggcg cgccgttcgt cgaagggcgc atcttgccgg gctcggagct
     1801 tactatcggc ggcacgacgg tgaccagccg ccagtgcgaa gccgcatcgc acgccacccg
     1861 ttcgcgaccg aagagcgatc cgtcgagcca gggcattcgc gtcttcgcgg cgctcgggtt
     1921 gccgctcgcc gggtacatgc tcctcacggc cgaacgtcac tcagccaaat ttcccgcggt
     1981 ggtcgaggca ccgccgcttt ggtccgctcc ggccgaggct tgcccggagc gcgagcgtga
     2041 ggcgagcgag gcgttcgggc aagccgaggt tctgcgtgc
//
LOCUS       DASTKS010001354         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_891576, whole genome shotgun sequence.
ACCESSION   DASTKS010001354 DASTKS010000000
VERSION     DASTKS010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891576"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFQ35_29065"
     CDS             <1..799
                     /locus_tag="VFQ35_29065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9934804.1"
                     /translation="PEIVFNGTSRSSPTVQSDVYSLACVAFELLTGRPPFQANNEVGV
                     LVQHATAPVPRPSSIRPDLPIAFDRVLLDALEKKPENRTPSAELFRRCLLEARRESLE
                     PVRILVAEDDDDFRDLLGLILLREFPGADVECVRNGAELLQCFDRKPASAILIDLQMP
                     ELDGFAVTRLLRERPNAANVPIIVLTAAGGPQEWARLSALGADRFMVKPVNLEDVVTL
                     TRRAVRERASWLPDSRPGGRGDARSAASHPTPTPTDPSEQPTRQVVR"
     gene            complement(784..>2019)
                     /locus_tag="VFQ35_29070"
     CDS             complement(784..>2019)
                     /locus_tag="VFQ35_29070"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9934805.1"
                     /translation="RKDCIVLATPGPDVRPLEARDSSALAPQGRRLMYRRSPVDLEDG
                     SPSEYRVTSSPSQRRALAIFALAAVATLVWLSLPLSSGLFLGTLLAFSLLRVHERLSR
                     RLGRPALSAVLLAVASALATLGSLVVLVYFIVARGMIAASNLAREFEPEGTFRKLLTR
                     LDEATRNSPFGPIDVTARVHTAAAEAASKLAELIPAIAGATFSALLTLFFTIMAAVFV
                     LRHWTGILARAERMLPLHRMHTRVVFAEFQKVGREVFIGTVLTGLAQGLLAGLGYAIG
                     GVPEPALLGALTAVCSLVPALGTLLVWVPVGIGLIVSGHVGAGIFELVWGALVVGILS
                     DYVIRPKLVGAGGHVPTLLTFISLFGGVKLFGLMGLIVGPVIAAVALALVRTYDREIC
                     EPDAEEALSQRNPKSQRTT"
BASE COUNT          386 a          657 c          684 g          292 t
ORIGIN      
        1 gcctgaaatc gtgttcaacg gaacgtcccg gagctcaccg acggtgcaga gcgacgtcta
       61 ctcgctcgcc tgcgttgcgt tcgagctctt gacgggccgt ccgccgtttc aagcgaacaa
      121 cgaggtcggc gtactcgtac aacatgcgac ggccccggtg cctcgtccga gcagtattcg
      181 cccggaccta cccatcgcct tcgaccgagt gctgctcgat gcgctggaaa aaaagcctga
      241 aaaccgcacc ccgagcgcgg agttgttccg gcgctgcctg ctcgaagcgc ggcgcgagag
      301 cctcgagccg gtgcgcatct tggtcgccga agacgacgac gacttccgcg acctgttggg
      361 gttgatcctg ttgcgcgagt tccccggtgc cgacgtggag tgcgttcgca acggagcgga
      421 gctgttgcag tgcttcgatc gaaagccggc ctcggcgatc ttgatcgatc tgcagatgcc
      481 ggagctcgac ggcttcgcgg tgactcgcct tttgcgagaa cgcccgaacg cggccaacgt
      541 cccgatcatc gtgctcacgg ctgccggtgg gccacaggag tgggcgcgcc tctctgcgct
      601 cggtgccgac cgattcatgg tcaaaccggt gaacttggaa gacgtggtga cgttgacgcg
      661 ccgcgcggtg cgggaacgcg cgagctggtt gcccgacagc cgtccaggtg gtcgagggga
      721 cgctcgctcg gcggcgtcgc atccgacccc gaccccgacc gacccctccg agcagccgac
      781 gcgtcaggta gtccgctgag acttggggtt gcgctgcgag agcgcctcct cggcgtcggg
      841 ctcgcagatt tcgcggtcgt aggtgcggac caacgcgagc gccacggcgg cgatgacggg
      901 gccgacgatg aggcccatca agccgaacag cttcacgccg ccgaacaacg atatgaacgt
      961 gagtagcgtc ggcacgtggc cgcccgcgcc gaccaattta gggcgaatca cgtagtcgga
     1021 gaggattccg accacgagcg cgccccacac cagctcgaaa atcccggcgc cgacgtgtcc
     1081 ggaaacgatg agcccgatcc ccacgggaac ccagaccagc aacgtcccga gcgctggtac
     1141 gagggagcag actgcggtca gcgcgccgag cagcgcgggc tccggtacgc ctccgattgc
     1201 gtagccgagc ccggccaaga gcccctgggc gaggccagtg agaacggtgc cgatgaacac
     1261 ctctcgcccc accttttgaa actcggcgaa gacgactcgc gtgtgcatgc gatgcaaggg
     1321 cagcatccgc tccgcgcgtg cgaggatccc cgtccaatgc cgcagcacga acacggccgc
     1381 catgatggtg aagaagaggg tgagcaacgc gctgaaggtg gcgccagcga tcgcgggaat
     1441 gagctcggcg agcttcgaag cggcttcggc ggcggcggtg tgcacacgcg ccgtgacgtc
     1501 gatcgggccg aacggtgagt tgcgagtcgc ctcgtcgagc cgtgtgagca gctttcgaaa
     1561 agtgccctcc ggttcgaact cgcgcgccag attgctcgct gcgatcatgc cgcgtgcgac
     1621 gatgaagtag accagcacca cgagcgaacc caacgtcgcg agcgccgagg ccaccgccag
     1681 taacaccgcc gaaagcgcgg gccgccccaa gcgccgagag agccgctcgt gcacccggag
     1741 cagtgagaag gcgagcagcg tcccgaggaa gagccccgac gagagcggca gggaaagcca
     1801 aaccagtgtc gcgacggcgg cgagcgcgaa aatagccagc gcgcgtcgct gcgacggcga
     1861 cgacgtcaca cggtattcgg aaggagagcc gtcttcgagg tccacgggtg agcgacgata
     1921 catcagacgg cgaccttggg gggcgagcgc cgacgaatcg cgagcttcca acggccgcac
     1981 gtctggaccc ggtgtggcga gaacgataca atctttccg
//
LOCUS       DASTKS010001355         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_444731, whole genome shotgun sequence.
ACCESSION   DASTKS010001355 DASTKS010000000
VERSION     DASTKS010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444731"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFQ35_29075"
     CDS             <1..499
                     /locus_tag="VFQ35_29075"
                     /inference="COORDINATES: protein motif:HMM:NF017330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase domain-containing protein"
                     /protein_id="HET9934806.1"
                     /translation="LHLDRVPRRYTVGAGHELDGEWDTSADGGAYDLWVLGPNGFHRH
                     FIGNVGRSRFRAAEPELRVEYDPRRDGLRLLLLNGGDEPCVFRLVANAYHPRWEPLFL
                     RVAARDHREHMLHIKQSANWYDFTVRVAGFDSFVRRFAGRMENGRNSLSDPALGGRAR
                     GEQNS"
     gene            771..1436
                     /locus_tag="VFQ35_29080"
     CDS             771..1436
                     /locus_tag="VFQ35_29080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934807.1"
                     /translation="MPGTETTFVCSVEVSKTKGFTFKLQNDAGKITQTVTLDGTTLVL
                     KVAGEKSTSTYTQTAEQVELKIVGESDTSTVTQTASAVTVKCKNFSVDAETVSVKSSK
                     TTEHQATETLSIKSTKDMSLTSSAKLSLTSTQDMSLTSSANLSQEATANASLKGNNTS
                     VEANLKLTGKGGVDIGLSATKLAINGDAKIDIQSPMTTVGDTLTTVKGQMVKVEGGLV
                     KLG"
     gene            1440..3113
                     /locus_tag="VFQ35_29085"
     CDS             1440..3113
                     /locus_tag="VFQ35_29085"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HET9934808.1"
                     /translation="MSQDAIERAFLTELESLEKFRISYTAQFPAVPLSHDDPDVRRLI
                     EAMAYFTARTRIAALRGLDDSVQRIFRQHFPSLLGPSPALAMLRATPKASFVEPAYLP
                     RGTEVLLTSPSVDGAPEQIFRFKTLAELRVLPLEVRSVEMVALRGRGYRLLIRLVASH
                     PTNAEVRSLSLHVNHLDDLRSSVTVLQALKDHALRCSVSYDPRAPEDAPGKPCSLQFG
                     LPEEADAVTESFEHPLQRARLRLRFPRQELFLNFHGMPPSRNWQHLTLAVDLSPDWPR
                     KLRLTSDDFELHTVPMLNLQRELANPIRCDGTKDRYPVLHPDAAGKYVPTWVIAAQRP
                     TKQGFVPLEPGVVGVSGDSYEVVTDGSGEDRRAWLILNLKSAFESPQVISADAFWHQP
                     GLRTADQSALRVGLHAQFVDGLEWALSGAIVPDADCEIDEDRDAQLELVSLKTLRFLG
                     HPELLSLLRALGVFAEKLFAPLARALADVRVTNKPMGQRSHGLKYRYELRFDSLELTD
                     LPRLALFCSWLLDVLVTWSAEEVIEIEAKVPNLGRPLLFERGLVRRSNG"
     gene            3106..3864
                     /locus_tag="VFQ35_29090"
     CDS             3106..3864
                     /locus_tag="VFQ35_29090"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HET9934809.1"
                     /translation="MDSKTRLWFAIDDAFGEIFHLCIEARAAELVLQQKQAEELVVRS
                     LAAAALPPPSRRAAEEGRYALASELARDAAFREQHPAGADLVMLRSHMRRRLNELRTT
                     LAEVLAENEVYYVLFPIVVYADELVSTATRGAVQRWEPMQGEFYEIDNGGELFYEVCA
                     ERLKQDETHPLVFETFYFCLMDGFCGMYPAGSKKIEDMKTQLRERILLAPVRFADKPR
                     EPPRAELVPFPVRYYALAFGLVVLAYIGFSIVAG"
     gene            3864..4238
                     /locus_tag="VFQ35_29095"
     CDS             3864..4238
                     /locus_tag="VFQ35_29095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934810.1"
                     /translation="MARFDPARIRHNFERTCGPAEAALPNRWAAVPQPLNPIPEGRRL
                     LGAIRELSERRYAAKWALLEPFFREAERLMSTLEVASQPAPSLPEPAKDRESPAALRE
                     ELCNSLRELEDLLEVVAEVGFG"
BASE COUNT          768 a         1335 c         1390 g          783 t
ORIGIN      
        1 gttgcacctc gatcgcgtcc ctcggcgcta tacggttggc gccgggcacg agctcgacgg
       61 cgaatgggac acgagcgcgg acggaggagc gtacgatctc tgggtgctcg ggccgaacgg
      121 ctttcatcgg cacttcatcg ggaacgtcgg gcgctcgcgt tttcgagccg cggagccaga
      181 gcttcgcgtc gagtacgatc ctcggcgcga tggcttgcgt ctactgctgc tgaatggcgg
      241 agacgagccg tgcgtgttcc gcctcgtcgc caacgcgtat cacccccgct gggaaccact
      301 gttcctgcgc gtcgcggctc gcgaccaccg tgagcacatg cttcacatca agcagagcgc
      361 gaactggtac gatttcacgg tgcgcgttgc tgggttcgac tcgttcgtgc gtcgcttcgc
      421 cggtcgcatg gagaacggtc gtaactcgct gagtgatccc gccttggggg ggcgcgcgcg
      481 cggcgagcag aacagttgat ggaagcgggg agcgagtgag cgctgcgtga gggccgcacg
      541 ctcgctcgag gggtaccggg gatgtggtga attccgacgt ggtcggtttc gtcatgccgt
      601 attccgtcag gcggttttcg tcgcggctct gcgtcgaaca tcgacttcgg cattcgttgt
      661 ctgatttttg gttcaatcgg ggcaggcgag tactgtcgcg acatcgtgaa cactctcgaa
      721 ctgcgctcgc cgtcgcggtt cgaaggtagc cacggtggcg actaaccagc atgccgggaa
      781 cagagaccac gttcgtgtgc tccgtcgagg tgagcaagac gaagggcttc accttcaagt
      841 tgcagaacga cgccggcaag atcacgcaaa cggtgacgct cgacggcacc accctcgtct
      901 tgaaggtggc gggggagaaa tcgaccagca cttacacgca aaccgcggag caggtcgaac
      961 tcaaaatcgt cggggaaagc gacacgagca ccgtgaccca gacggccagc gccgtgaccg
     1021 tgaagtgcaa gaacttcagc gtcgacgcgg agacggtgtc ggtgaagtcg agcaaaacca
     1081 ccgaacacca ggcaaccgag accctctcga tcaagagcac caaggacatg agtctgacct
     1141 ccagcgcgaa gctcagcctg acgagcaccc aggacatgtc gctcacgtcg agcgccaatc
     1201 tgagccagga ggcgaccgcc aacgcctcgc tcaaggggaa caacacgagc gtcgaggcga
     1261 acctcaagct cacgggtaag ggaggcgtcg acatcggtct ctcggcgacc aagctcgcca
     1321 tcaatggcga cgcgaagatc gacatccaat cgccgatgac gacggtgggg gacacgctga
     1381 ccacggtcaa aggtcagatg gtcaaggtcg aaggcgggct cgtgaagctc gggtgaagca
     1441 tgtcgcaaga cgctatcgaa cgcgcatttt tgaccgagct cgagtccctc gagaagttca
     1501 ggatctcgta tacggcgcag tttccggccg tccccctttc gcacgacgac ccggacgtgc
     1561 ggcgtctcat cgaggcgatg gcctatttta cggcgcgcac gcgcatcgcc gcgttgcgtg
     1621 gcttggacga cagcgtacag cgcattttca ggcagcattt cccgtcgttg ctgggcccga
     1681 gccccgcgct cgccatgttg cgcgcaacgc cgaaggcgag cttcgtcgag ccggcgtacc
     1741 ttccccgcgg gaccgaggtg ctgctgacct cgccgtcggt agacggtgct cccgagcaga
     1801 ttttccggtt caagacgctg gccgagcttc gagtgttacc cctcgaggtt cgttcggtag
     1861 agatggtggc gctgcgtggc cgcggttacc gactgctgat tcgattggtg gcgagccacc
     1921 caaccaacgc cgaagtcagg agcctcagtc tgcacgtgaa ccacctggac gatctccgtt
     1981 cgtctgtcac ggtcttgcaa gcgctcaagg atcacgcgct acgctgctcc gtgtcctacg
     2041 acccgagggc gccggaggac gcgcccggca aaccctgctc gctccagttc ggtctacccg
     2101 aggaagcgga cgcggtaacg gaatcgttcg agcatcctct gcaacgcgct cggttgcggc
     2161 tgcgatttcc ccgacaagag ctgttcctga attttcacgg gatgccgccg tcgcgcaact
     2221 ggcagcacct cacgttggca gtggacttgt cgccagactg gccgcggaag ctgcggctca
     2281 cttcggacga cttcgagctc cacaccgtcc cgatgctgaa tttgcagcgc gagctcgcaa
     2341 acccgattcg ctgcgacggg acgaaggatc gatacccagt ccttcaccca gacgcggcgg
     2401 gaaagtacgt gccgacgtgg gtcatcgccg cgcagcggcc gaccaagcaa ggtttcgtgc
     2461 cgctcgagcc cggggtggtc ggggtttcgg gagatagcta cgaggtggtc acggacggga
     2521 gcggggaaga ccgccgagca tggctgatcc tgaatctaaa gagcgcgttc gagtccccgc
     2581 aggtgatctc ggcagacgcg ttttggcatc aacccggctt gcgcacggca gaccaatctg
     2641 cgctccgagt gggcttgcac gcgcagttcg tcgacgggct cgagtgggcc ctctcgggcg
     2701 cgatcgtccc cgacgcggac tgcgagatcg acgaagaccg cgacgcccaa ctcgagttgg
     2761 tctcgctgaa gacgctgcgg tttctgggtc acccggagct gctctcgctg ctgcgggcgc
     2821 tcggcgtgtt tgccgaaaag ctgttcgcac ccctcgcgcg cgctctcgcc gacgttcgcg
     2881 tgaccaacaa accaatgggc cagcgtagcc atggcctcaa gtatcgctac gagctcaggt
     2941 tcgactctct cgagctcacg gacttgccgc gtcttgcctt gttctgctcg tggcttctgg
     3001 acgtactcgt cacgtggagc gcagaagagg tgatcgaaat cgaagccaaa gtgccgaact
     3061 tgggccgccc cctgctcttc gaaaggggtc tcgtgaggcg aagcaatgga tagcaagacc
     3121 cgtctctggt tcgcgatcga cgacgcgttc ggcgagatct tccacctttg tatcgaggcg
     3181 cgcgccgccg agctcgtctt gcaacagaag caagccgagg aattggtggt gcgttcgctg
     3241 gcagcggcgg cgttaccgcc accgtcgcgc cgggcggccg aagagggacg ctacgctctc
     3301 gcgagcgagc tcgctcggga cgcggccttt cgagagcagc acccggccgg tgcagacctc
     3361 gtcatgttgc ggtcgcacat gcgacggcgc ctgaacgagc ttcgcacgac tctcgccgag
     3421 gtgttggccg aaaacgaagt ctactacgtg ctcttcccca tcgtcgttta tgccgacgag
     3481 ctcgtgtcga cggccacgcg cggggcggtg cagcgctggg agcccatgca gggcgagttt
     3541 tacgagatcg ataacggcgg cgagctgttc tacgaagtct gcgccgagcg cttgaagcag
     3601 gacgagaccc accccctcgt cttcgagacc ttctacttct gtctgatgga cggattttgc
     3661 ggcatgtatc ctgcgggctc gaagaagatc gaagacatga agacacagct ccgcgagcgc
     3721 attctgctcg cgccggtacg cttcgccgac aagccgcgag agcccccgcg cgcggaactc
     3781 gtgccctttc cggtgcgata ttacgcgctc gcgttcgggc tcgtggtgct cgcgtacatc
     3841 ggcttttcga tcgtggccgg ctgatggctc gcttcgaccc ggctcgcatt cgacacaact
     3901 tcgagcggac ctgcggtccc gccgaggcgg ccctgccgaa tcgctgggcg gcggttcccc
     3961 agccgctgaa cccgatcccg gagggacgtc gcttgctcgg agcgatccgc gaattgtcgg
     4021 agcggcgcta cgcggcgaag tgggcgttgc tcgagccgtt ctttcgagag gcggagcgcc
     4081 tgatgagcac cctcgaggtc gcgtctcagc ctgcgccgag tctgccggaa cccgccaagg
     4141 accgcgagtc accagccgcg ctccgtgaag agctttgcaa ttcgctacgc gagctcgagg
     4201 acttactcga ggtggtcgcg gaggttggct tcggatgaag tcgcggtcag ctcgcgacct
     4261 tgtcgagctc gtggtc
//
LOCUS       DASTKS010001356        11081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_444840, whole genome shotgun sequence.
ACCESSION   DASTKS010001356 DASTKS010000000
VERSION     DASTKS010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444840"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFQ35_29100"
     CDS             complement(<1..710)
                     /locus_tag="VFQ35_29100"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET9934811.1"
                     /translation="MPADDDALRPSDYLVSSLLTDAGFRHAFFTRTGGVSENEFASLN
                     FSVGVGDTPAHVEENLRRAARMLRVAEGRIYFASQVHGAAAELADPAIDFDAFVCREA
                     DVVFGRSPDVACGVRTADCVPILLAAERSGHVAAVHAGWRGVVRGAVRAGLAALERSG
                     ADASDVVAAIGPHISVAAFEVSDEVASELVAASPDSNVVDRSRARPHVDLRRIVRAQL
                     ESFGVRRGAIDDVFGCTYA"
     gene            802..1899
                     /locus_tag="VFQ35_29105"
     CDS             802..1899
                     /locus_tag="VFQ35_29105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9934812.1"
                     /translation="MRPKDPFVGRSILGGQYEVLEKIGTGGMGSVYKAAQPAEQRQVA
                     LKILHPKLAGRKDLTSRFRREARAMMQLSHRNTVKVYNYGELEEDGSLYIVMELLEGR
                     NLNQVVRREGPLPPERAIPILIQVCGALQEAHNLGIVHRDLKPENIFLCKHDGPIDFP
                     KVLDFGLAKVTERQMQPGSIILTQEGMVFGTPEFMSPEQAQGRTLDARSDIYSLAVIL
                     YELLTGKLPFSARTPMEYIQKHVTDAVIPFAERAPDRAFPRGLEEVIVKALAKRPEDR
                     YQTAEAFAEALRPFGGPAAAAIPRDPVGKAPPSARAPGSVPVVGSTVPAVSGSASASA
                     RPSTSVLVGVAAACLVLGVALAVLAMRLFGR"
     gene            2010..4400
                     /locus_tag="VFQ35_29110"
     CDS             2010..4400
                     /locus_tag="VFQ35_29110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET9934813.1"
                     /translation="MERDEALRLLKKGAKYGGLGIGAATLVGAGAVFATIHHYEANLP
                     SVEQLKTGYRPPQVTRVLARDGSLLGSLFTERRTVIAFADLPPHVKLSFLAAEDASFY
                     EHQGLNYFGMLRALLANLRAHGTRQGASTITQQVVKNLLLTPERTYERKVKETILAKR
                     LEQELSKDEIFSLYLNHIYLGHGRYGVEEAARYYFGKKARELDLAESALLAGLVAAPE
                     RFSPRVDAEKALGRRRYVLKQLVEKGFVTRELYDQASAEPLRLAPGADEESELCPEIV
                     AYARRTLKSVAGDAADKGGYTVTTTIDPRLQAAARKALRDGLGAYASRQKLEPPLTLG
                     TRRLWGPAFSGTPQPNNIYIGRVRSLDDEKNTIDVEIGDAVGRVALAREGRYNPKHLS
                     PSAFTKVGALLRVSLLSAVEHGQAAVPLRLELGPEGALVAVDVRSRDVLALVGNEEAV
                     AGGLDRATQAHRQPGSSFKPFLYGYALASRRLTTSSVLDIPGTPAKNGKPAKEARRLS
                     LRKAIAQSDNDAAELLLSTVGAPNVIEWARSLGIESRMEPTRSLALGAYEVYPLEIVA
                     AYAAFASGGEVAPPRLIRKIVGPDGKELSLPSAPPSRRAISAEEAYLVTSLLRGVVES
                     GTAKRAASLGRPLAGKTGTTNLAKDCWFIGYSTEIVAGAWVGYDNPMPLGWGESGATT
                     SLPIWMSFMKAAHEGRPATDFPRPSGIVTASIDPATGLLAYPGQTDAIEEEFLDGTVP
                     NETAKPPEAPTSPAAGVDDEANEGRTVAAPTPDPPAPKPNVPAAATAGGATEPPPF"
     gene            4497..5558
                     /locus_tag="VFQ35_29115"
     CDS             4497..5558
                     /locus_tag="VFQ35_29115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934814.1"
                     /translation="MKEWCKPVLLGLPFLLLSCEKESPRSDAPKAAASSGEKATALDP
                     DLAQAMAAASAAAPAQPGTAAAGGPPATGIFAPGAADREVKKGDPPKLTLGNEGKEPR
                     ITLTPVQPKPGSKQSGIISVVQQQADGSGIPIEFAVTFEAQKPKADAAPTAPVPVTVK
                     VTGAKIAVAGAPKDLEATVAKLKGSKIDYEVAADGAGSNYRFELSKAAPADLGDTIRS
                     LSDVIAVVTVPFPDKPVGAEGFWMATSREGVLGLDLVTYRLIKVERIDGQKVTLNVNT
                     KRYSATPNFDLPGLQPGAPHSLAEFQALAQGTVELVAGKGFPVGGNQNSSLRAQLQDP
                     ANPRARGMLQIDTQAELKF"
     gene            complement(5620..5925)
                     /locus_tag="VFQ35_29120"
     CDS             complement(5620..5925)
                     /locus_tag="VFQ35_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9934815.1"
                     /translation="MTFVVTDNCKGCRFTDCVAVCPVDCFHGDGEMLYIDPGECIDCG
                     ACVPECPVEAIFDETQIPDDKKQYIKLNADKAPSLPVVNQKTDPLPGAEERKQKLGF"
     gene            complement(6073..6693)
                     /gene="msrA"
                     /locus_tag="VFQ35_29125"
     CDS             complement(6073..6693)
                     /gene="msrA"
                     /locus_tag="VFQ35_29125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9934816.1"
                     /translation="MLTRRSLLIGSLLGFAAACTRRSPPAPAAAPATSASADAELETT
                     ILAGGCFWGMEEILRKIPGVVSTEAGYAGGKAGVNYDDVHGGASGNAEAVRIVFDSKK
                     LSFEELLAKWFFRMHDPTTQNRQGNDVGSQYRSAIFVTSPAQRETAERVKRRVEASGV
                     WGKPIVTEIAPATTFTRAEEVHQRYLEKNPDGYTCHYLRDFGGTSL"
     gene            complement(6763..7368)
                     /locus_tag="VFQ35_29130"
     CDS             complement(6763..7368)
                     /locus_tag="VFQ35_29130"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9934817.1"
                     /translation="MLLEINPVTPEPRKIKRAVDALQSGEVIAYPTDTCYGLGCDLFN
                     KKAVDKLYQIKGMERSQMLAFVCQNIGDVAKYAVVDDRTFRVLKQFLPGPYCFILNAT
                     REVPRIVQTPRKTVGVRVPNNPVALALAAELGRPIISSTAARHGEDPNPDPREIETEF
                     RGLALVLDAGAGGVTPTSVVDLSGGEPKVVRVGAGDVSRFD"
     gene            complement(7429..9468)
                     /locus_tag="VFQ35_29135"
     CDS             complement(7429..9468)
                     /locus_tag="VFQ35_29135"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET9934818.1"
                     /translation="MPRRPDENRKNRDRAELRRSGLVLGDRTLPLLAGSVHYFRLEQS
                     AWRPALEALQELGLGIVDTYIPWGVHEVSAGVYDFGERDPKLDIVRFVELAGELGLVV
                     IVRPGPHINAELTHFGIPERIIWDAECQALSARGRPVVLPVPPLAFPVPSYASKKFLS
                     EAQHWLAAVGKLLAPLCWPAGPIVLCQVDNEGAMYFRDGVYDQDYHPDAIKNYHKFLR
                     KKYETREELENALGYSVLNFEIDPPRKLEAETSRDLTRHLDWAEAQEELLASCFKKLA
                     STLRDTLIDVPYSHNFPIGEALTPLDPGRVREVVDLVGLDYYHVATPEQRAEIARRTS
                     ELSVRSDTCEVPAFACELGAGFPPFFPALTQVDNEFTVLTALAYGLRGYNLYMAVERD
                     RWIGAPFDRHGRARPSAEFWSQLSTAFKRLAFHELERRTPVHIVIPRSLRRLHRVLHG
                     FGPLSAVMFQIAGGGVTDAQFEDELDLGAPFALDAERFARWLENELERRRIPFAVVGG
                     DLIEASIERARWSIVACAGGLERDITKAVSRGLKDGRAISIGPHFPQRDETLIPREPP
                     GALRHAFGRTLPTLLSSDATELSMTIDRAVEALGLETVPASPDGISVTLHHDAAGRAR
                     VMFVINPTEYAVEAKVGANGESVAEDALDGTVFHAKNQAFELPVPARTVRMLALR"
     gene            9983..10561
                     /locus_tag="VFQ35_29140"
     CDS             9983..10561
                     /locus_tag="VFQ35_29140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET9934819.1"
                     /translation="MFALRRVAPLWAVLVVACASEPPRADVPPPRDKGSRSSATETGP
                     ASTSAPPLAHAASQDVEESPDTLSQRYARSPALARFSGQVSYYSDALAGRSTASGEPY
                     QPRAFTAAHRSLPFGTIVRVTSDVTGKSVYVRINDRGPFVRGRVLDLSRAAAERLGLL
                     GRGVLKVRAEIVENGPTKPRARRRTRKRKSSH"
     gene            complement(10920..11078)
                     /locus_tag="VFQ35_29145"
     CDS             complement(10920..11078)
                     /locus_tag="VFQ35_29145"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9934820.1"
                     /translation="MWGPNQSEQSHYVRVHMAELRKKIEANPARPEWLVTEPGVGYRL
                     RDRPTETK"
BASE COUNT         1919 a         3621 c         3685 g         1856 t
ORIGIN      
        1 gcgtaagtgc agccgaaaac gtcgtcgatg gcgcctcgcc gcacaccgaa gctttcgagc
       61 tgtgcacgca cgatgcgccg aaggtctaca tgaggacgcg ctcggctccg gtcgactaca
      121 ttggagtcag gagaggccgc gacgagctca cttgcaacct cgtccgagac ttcgaacgcc
      181 gcgacagaaa tgtgcggtcc gatcgcagcg accacgtcgc ttgcgtcggc gcccgaccgc
      241 tcgagtgccg cgagcccggc tcgaaccgct ccgcgaacga cgcctcgcca accggcgtgt
      301 acggcggcga cgtgaccgct ccgctccgcg gcgagcagga tggggacgca gtcagccgta
      361 cgaacaccac aggcgacgtc gggcgaacgg ccaaagacta cgtcggcttc acggcagacg
      421 aacgcatcga agtcgatagc gggatcggcc agctcggcgg ctgcgccgtg cacctgcgaa
      481 gcgaagtaaa tccggccttc ggccacccgg agcatgcgcg cggcgcgacg caggttctct
      541 tcgacgtggg ccggtgtgtc tcccacgcca acggaaaagt tgagggaggc gaactcgttt
      601 tcggatacgc cgcccgtgcg cgtgaaaaac gcgtgccgaa acccggcgtc agtcaataac
      661 gaactgacca gatagtcgct cgggcgtagc gcgtcgtcgt cggccggcat gaggggccga
      721 gcctatccga tcacgcgagt ttccggggga gagagcgcga tttcaccgca ctcttccgtt
      781 ttttggtaag tgtcgggacg catgcgcccg aaggaccctt tcgtcggccg aagcattctc
      841 ggcggccaat acgaagtgct ggaaaagatc ggcaccgggg gcatggggtc cgtctacaaa
      901 gcggctcaac cagccgagca gcgccaggtc gcgctgaaga ttctccatcc aaagcttgcg
      961 ggtcgcaaag acctgacatc gcgcttccgc cgcgaggcgc gcgcgatgat gcagctttct
     1021 caccgcaaca cggtcaaggt ttacaactac ggtgagctcg aagaggacgg gtcgctctac
     1081 atcgtcatgg agctgctcga gggcaggaac ctcaatcagg tcgtgcggcg cgaaggcccg
     1141 ttgccgcccg aacgcgccat tccgattttg atccaggtgt gcggcgctct ccaggaagct
     1201 cacaacctcg gaatcgttca tcgcgatctc aagccggaga acatctttct ctgcaagcac
     1261 gacgggccca tcgattttcc gaaggtgctc gatttcggtc tcgcaaaggt gaccgaacgc
     1321 cagatgcagc ccggctcgat catcttgacg caagagggca tggtgttcgg tacgcccgag
     1381 ttcatgtcgc cggagcaagc tcaaggccgc acgctcgacg cgcgcagcga tatctactcg
     1441 ctcgccgtga tcttgtacga gctgctcacc gggaagctcc cgttttcggc gcgcactccc
     1501 atggagtaca ttcagaagca cgtcaccgac gcggtgatcc cgttcgccga gcgcgcccca
     1561 gaccgcgctt tcccgcgtgg gctcgaagag gtgatcgtga aggcgctcgc gaagcgcccc
     1621 gaggaccgat accaaaccgc cgaggctttc gctgaggcgc ttcggccgtt cggggggccc
     1681 gccgccgccg cgattcctcg cgatccggtg ggcaaggcgc cgccgtcggc gagagcgccc
     1741 gggtcggtgc cggtcgtcgg cagcaccgtt ccggcggtga gcgggtctgc ctccgcgtcg
     1801 gcgcgcccga gcacgtcggt gttggtgggg gttgcggccg cctgtctggt gctgggcgtc
     1861 gccctcgccg tgctcgcgat gcgccttttt ggccggtgac cgcgacttgt cgcggttgtt
     1921 cgccggcccc gcccccaaaa cacacaggct gagtatgcgg cccgtggcga cttgtcgcgg
     1981 ttgttcgccg atgtgataag ccccgagcga tggaacgcga cgaggcgctg cgactcctca
     2041 agaagggggc gaagtatggg ggcctcggga ttggagcggc gacgctggtc ggggctggcg
     2101 cggtgtttgc gaccatccac cattacgaag cgaacctgcc gagcgtggag cagctgaaga
     2161 ccggctaccg cccacctcag gtaacgcggg tgctggcgcg tgacgggagc ttgctcggga
     2221 gcttgttcac ggagcggcgt accgtcatcg ccttcgcgga tctaccgcct cacgtgaagc
     2281 tatcgttttt ggcggcggaa gacgcgagct tctatgagca tcagggcctc aactatttcg
     2341 gcatgctccg ggcgctgctc gcgaacctgc gtgcgcacgg cactcgacag ggcgcgagca
     2401 ccatcactca gcaggtcgtg aagaacctgc tgctaacacc cgagcgcacc tacgagcgca
     2461 aagtcaaaga gacgattttg gcgaagcggc tcgagcaaga gctctcgaaa gacgaaatct
     2521 tttctctcta tttgaatcac atttatctcg ggcacggccg gtacggcgtg gaagaagcgg
     2581 cgcgctacta cttcggcaag aaggcccggg agctcgacct cgccgaatcg gcgttgctgg
     2641 ctgggctcgt cgccgcgccg gagcgatttt ctccgcgcgt cgacgccgag aaggcgctcg
     2701 gccgccggcg ctacgtgttg aagcagttgg tcgagaaggg gttcgtcacg cgtgagctct
     2761 acgaccaagc gagcgcggag ccgctgcggc tcgcccccgg cgccgacgaa gagtccgagc
     2821 tttgcccgga aatcgtcgct tacgcacggc gcacgctgaa gtcggtcgcg ggcgacgccg
     2881 cggacaaggg cggctacacg gtgacgacga ctatcgatcc gcgcttgcag gcggctgctc
     2941 gcaaggcgtt gcgcgacggc ctcggcgctt acgcatcgcg ccagaagctc gagccgccgc
     3001 tcacgctcgg cacccggcgg ctgtggggtc ccgccttttc gggcacgccc cagccgaaca
     3061 acatctacat cgggcgcgtc cgctcgctcg acgacgagaa gaacacgatc gacgtcgaga
     3121 tcggcgacgc ggtgggacgc gtcgctctcg cgcgcgaggg gcgctacaac ccgaagcacc
     3181 tgtcaccgag cgccttcacc aaagtcggtg cgctgcttcg tgtctcgctc ctctcggcag
     3241 tggagcacgg gcaagccgcg gtgccgcttc ggctcgagct cgggcccgaa ggcgcgctcg
     3301 ttgccgtcga cgtgcggtcg cgcgacgtgc tcgcgctcgt cggcaacgaa gaggccgtcg
     3361 ccggtgggct cgatcgcgcg acgcaggcgc accggcagcc gggctcttct ttcaagccat
     3421 ttctctacgg ttatgcgctc gcgtcgcggc gcctgaccac gtcgagcgtg ctcgatatcc
     3481 ccggcacgcc cgcgaagaac gggaagcccg cgaaagaggc gcggcggctc tctttgcgca
     3541 aggccatcgc tcagagcgac aacgacgctg cagagctttt gctgtcgacg gttggcgcgc
     3601 ccaacgtgat cgagtgggcg cgctcgctcg gcatcgaatc gcgcatggag cccacgcgtt
     3661 cgctcgcgct cggcgcttac gaagtgtacc cgctcgaaat cgtcgcggcg tacgccgcgt
     3721 tcgcgagcgg cggcgaggtg gcaccgcctc ggctcattcg gaagatcgtg ggtcccgacg
     3781 gcaaggagct cagcttgccc tccgctcctc ccagccgccg cgccatcagc gccgaagagg
     3841 cgtacctcgt cacgagcctg ctgcgcggag tcgtcgaatc gggcaccgcg aagcgcgccg
     3901 cgagcctcgg tcgcccgctc gccggaaaga cgggcaccac gaacctcgcc aaggattgtt
     3961 ggttcatcgg atactcgacg gagatcgtcg ctggtgcctg ggttggctac gacaacccca
     4021 tgccgctcgg gtggggagag tcgggcgcga ccacctccct gccgatttgg atgtccttca
     4081 tgaaggccgc gcacgaggga cgacctgcta ccgactttcc gcggccgagc ggtatcgtca
     4141 ccgccagcat cgatcccgcc acgggtctgc tcgcgtaccc gggccaaaca gacgccatcg
     4201 aggaagaatt cctggacggc accgttccga acgaaacggc gaagccaccc gaggctccga
     4261 cgagcccggc cgcgggcgtg gacgacgaag ccaacgaggg gcgaaccgtg gccgccccga
     4321 cgcccgaccc tcccgcgccc aagcccaacg tgccggctgc cgcaacggcc ggaggggcca
     4381 ccgagccgcc gccattctga ccgaggcacg cgcggggtgg acattggtcg gttgatgagt
     4441 aaaacggacg ccgttccatc cgcccaagcg agcttgggtc aatccgagag gcgaaattga
     4501 aagagtggtg caagcctgtt ctgttgggtc tgccgttctt gttgctctcg tgtgaaaaag
     4561 agagcccgcg atcggacgcg ccgaaagccg cggcgtcgag cggggagaag gcaaccgcgc
     4621 tcgaccccga tctcgcgcaa gccatggcgg ctgcatctgc cgccgcaccg gctcagccgg
     4681 gaacggcggc tgctggcggt ccccccgcta cggggatctt cgcgccgggt gccgcggatc
     4741 gagaagtcaa aaagggcgat ccgcccaagc ttactctcgg caacgagggc aaggagccgc
     4801 gcatcacgtt gacgccggtg caaccgaagc cgggcagcaa gcaaagcggc atcatctcgg
     4861 tcgtccagca gcaggccgac ggcagcggca tcccgatcga atttgcggtc acgttcgagg
     4921 cgcagaagcc caaagcggac gcggcaccga ctgcacccgt gcccgtcacg gtgaaggtga
     4981 ccggcgcgaa gatcgccgtc gcgggcgccc ccaaagatct agaggcgacc gttgccaagc
     5041 tcaagggctc caagatcgac tacgaggtcg ccgcggatgg agcgggcagc aactaccgct
     5101 tcgaattgtc caaggcggcg ccggcagatc tgggcgacac gattcgctcc ctctccgacg
     5161 tgatcgcggt cgttacggtg ccgttccccg acaagcccgt cggagccgaa gggttctgga
     5221 tggccacgag ccgcgaaggc gtgctcgggc tcgatctcgt cacctaccgg ctcatcaagg
     5281 tcgagcgcat cgacggccaa aaagtgacgc tcaacgtgaa cacgaagcgt tactcggcga
     5341 cgcccaactt cgaccttcct gggttgcaac ccggcgcgcc ccactcgctc gccgagttcc
     5401 aagcgctcgc tcagggtacg gtcgagctgg tcgccggcaa gggcttcccg gttgggggca
     5461 accagaactc gtcgctacgc gcgcagctgc aggatccggc gaacccccgc gcgcgcggca
     5521 tgcttcaaat cgatacgcag gccgagctga agttctgagc aaaagcgtcg ggcacgggcg
     5581 acggctcgaa acgtcgcccg cgtcgttcgc ccacggcgct tagaaaccga gcttctgctt
     5641 gcgctcttcg gcgccgggca gcggatccgt cttttggttc acgacgggga gcgacggcgc
     5701 tttgtcggcg ttcagcttga tgtactgctt cttgtcgtcc gggatctgcg tctcgtcgaa
     5761 aatggcttcg acggggcact ccggcacgca cgcgccgcag tcgatgcact caccgggatc
     5821 gatgtagagc atctcgccgt cgccgtgaaa gcagtctacc gggcagacag ccacgcagtc
     5881 ggtgaaacgg cagcctttgc agttgtcggt gacgacgaag gtcatgggag gcccttatta
     5941 gctccccgct cggcgcggat caatatctag ggccgcgggc ggcgacgacg cgtgacgctt
     6001 tgtatcgggg cgcgagggat cctcgggccc tcgcgactta ccgcggcggg ctagagaaga
     6061 gtcgcgctcg gctcaaagac tcgtgccccc gaagtcccga aggtagtggc acgtgtagcc
     6121 gtcggggttc ttctcgaggt atcgctggtg gacctcttcg gcccgggtaa aggtcgttgc
     6181 cggagctatc tcggtgacga tgggcttgcc ccagactccg ctcgcctcga cgcgtctctt
     6241 cacccgctca gccgtctcgc gctgggcggg cgacgtcacg aaaatggcgg agcggtactg
     6301 ggagcccacg tcgttgccct ggcggttttg ggtcgtcggg tcgtgcatgc ggaagaacca
     6361 cttcgcgagg agctcctcga acgaaagctt cttcgagtcg aacacgatgc ggaccgcctc
     6421 cgcgttgccc gacgcgccac cgtggacgtc gtcgtaattc actcccgcct tgccaccggc
     6481 gtaaccggcc tctgtcgaga cgactccagg gatctttcgg aggatctcct ccattcccca
     6541 aaagcacccg ccggcgagga tggtggtttc gagttcggcg tcggcgctag cggaggtggc
     6601 cggtgcggcg gcgggagcgg ggggcgagcg ccgagtgcag gccgcggcga aacccaagag
     6661 cgagccgatg agcagactgc gacgcgtgag cattcgaaag ctttacgcga ggcaccggga
     6721 aacggttacg cgcgccgagc tccgcgcgct gcgccccgcg cgtcagtcga accggctgac
     6781 gtcgccggcg cccacgcgta cgaccttcgg ctcgcctccg ctcagatcga ccacgctggt
     6841 gggcgtgacg ccgccggcac ccgcgtcgag aaccagcgcg agccctcgga attccgtctc
     6901 gatttcgcga ggatctgggt ttgggtcttc accgtggcgt gcggcggtgc tcgaaatgat
     6961 cgggcggccg agctccgccg cgagggcgag cgccaccggg ttgttgggca cgcgcactcc
     7021 gacggtcttt cggggcgtct gtacgattcg cggcacctcg cgggtcgcgt tgagaatgaa
     7081 gcaatacggt cccggcaaga actgcttcag cacgcggaac gtgcggtcgt ccacgacggc
     7141 gtatttcgcg acgtcgccga tgttctggca gacgaaggcg agcatctgcg agcgctccat
     7201 acctttgatt tggtagagct tgtcgacggc cttcttgttg aacagatcgc agccgagccc
     7261 gtagcaagtg tcggtgggat acgcgatgac ttcgccggat tgaagtgcgt cgacggcgcg
     7321 cttgatcttg cgcggctcgg gcgtgactgg attgatttcg aggagcatgg gcggggcggg
     7381 tgtggaaggg tcccagccgc cagacccccg gcgaactccg gatgctgcct agcgcagggc
     7441 gagcatgcgc acggtcctcg ctggaacggg cagctcgaat gcctggttct tagcatgaaa
     7501 caccgttccg tcgagggcgt cctctgccac gctctcgccg tttgcgccca ctttggcctc
     7561 gactgcgtat tccgtggggt tgatgacgaa catcacacga gctcgccccg cggcgtcgtg
     7621 gtgaagcgtg accgatatgc cgtcgggact tgcgggtacg gtttccagcc ccagcgcttc
     7681 gacggcccga tcgatcgtca tgctgagttc ggtcgcgtcc gagctcaaca gagtgggcaa
     7741 cgttcgccca aaagcgtggc gcagcgcgcc gggaggctct cgggggatca atgtttcgtc
     7801 tcgttgcggg aaatgaggac cgatgctgat ggcgcgtccg tccttcaacc ctcggctcac
     7861 tgccttcgtg atgtcgcgct cgaggccgcc cgcacaagcg acgatgctcc agcgcgctcg
     7921 ctcgatgctc gcttcgatga gatcgccgcc cacgaccgcg aagggaatgc ggcggcgttc
     7981 gagctcgttt tcgagccagc gcgcgaagcg ctcggcgtcg agcgcgaacg gagctccgag
     8041 gtccagctcg tcttcgaatt gcgcgtccgt cacaccgccg cccgcgattt gaaacatgac
     8101 cgcgctgagc ggaccaaagc cgtgcaagac gcggtgcagg cggcgcaggc tccgcgggat
     8161 cacgatgtgc accggggtgc gccgctcgag ttcgtggaag gcgaggcgct tgaaggcggt
     8221 cgacagctgg ctccaaaact ccgccgaggg ccgcgcgcgc ccgtgccgat cgaagggagc
     8281 gccaatccaa cgatcgcgct cgacggccat gtacaggttg tagccacgga ggccatacgc
     8341 gagcgccgtg agcaccgtaa actcgttgtc gacctgcgtc agcgccggaa agaacggcgg
     8401 gaagccggcg ccgagctcgc aagcgaaggc cggcacctca cacgtgtccg agcgcaccga
     8461 aagctcgctc gtgcgccgcg cgatctcggc gcgctgctcg ggagtcgcga cgtggtagta
     8521 gtcgagcccg accagatcca ccacctcgcg cacgcggccg gggtcgagcg gcgtgagcgc
     8581 ttcgccgatg gggaagttgt gtgaatatgg cacgtcgatc agagtgtcgc gcagcgtgct
     8641 cgcgagcttc ttgaagcagc tcgcgaggag ctcctcttgc gcttccgccc agtcgaggtg
     8701 cctcgtgaga tcgcgcgacg tctccgcctc gagtttgcgc gggggatcga tctcgaagtt
     8761 caaaaccgaa taaccgagcg cgttctcgag ctcctcgcgc gtctcgtatt ttttgcgcaa
     8821 gaacttgtga taattcttga tcgcgtccgg gtggtaatcc tggtcgtaaa cgccgtcgcg
     8881 gaagtacatc gcgccttcgt tgtcgacctg gcagagcacg atgggcccgg cgggccaaca
     8941 cagcggcgcg agcagcttac ccaccgccgc cagccagtgt tgcgcctcgc tcaggaactt
     9001 cttgctggcg tagctcggaa ccgggaacgc gagcggcggc accggtagca ccacggggcg
     9061 accgcgcgcc gagagcgctt ggcactctgc gtcccaaatg atgcgctcgg ggatgccgaa
     9121 gtgcgtgagc tccgcgttga tgtgcggacc tggccgcacg atcacgacga gtccgagctc
     9181 tccggcgagc tcgacgaagc gcacgatgtc gagtttcgga tcgcgttcac cgaagtcgta
     9241 gactccggcg ctcacttcgt ggacgcccca agggatgtac gtatcgacga taccgagccc
     9301 gagctcttgc agtgcctcga gggctgggcg ccacgccgac tgttcgagcc gaaagtagtg
     9361 aacgctcccg gcgagcaacg gcagggtgcg atcgccgagc acgagcccgc ttcggcgcag
     9421 ctcggcgcgg tctctgttct tgcggttttc gtcgggccta cgtggcatgc agcgtcccta
     9481 tatcatgcgg gagccacgcc gcttgcgagt tcggaaacat gtcacgtcgg cttcgctcga
     9541 tgccacgcct tgcttcgctc agcgctgacg ccccgcagtt ttgcgcacgg aaatgtcgtc
     9601 gaggcgcttt cgactcttcg gggcgggtct cggctcagag ctccgagcgg gttcggtccg
     9661 gccgctcgcg tcgtgcgact gcgtcccaga gcagcgcgca gtcggcgcac gaacatggct
     9721 gcggcaggtc gacgaccatc gaacgaactc ttcgcggctc gagtggaagc gaccgttccg
     9781 tgccgagcga gcagctctcg agtgagacga acgttacctg cgctcgagat gcgatgaacc
     9841 tgagtgcgca gtaaccctca tccagtccta gacgcgaacc tcggataagt cgcttcaatt
     9901 cacacggatg atttcacagt cgctctgact ggatttttat tcgcgacttc cgaatcgaaa
     9961 actccatgct agaaatgcgc acgtgtttgc gcttcgtcgc gtcgcgccgc tttgggcggt
    10021 gttggtcgtc gcctgcgcgt ccgagccacc gcgggccgat gtgccgccgc cgcgcgacaa
    10081 aggctctcgc tcctccgcaa cggagacggg gcccgcttcg acgagcgctc caccgctcgc
    10141 gcacgcagct tcgcaagacg tcgaagagag cccagacacg ctcagtcaac gctacgcccg
    10201 aagccccgcg ctcgcgcgtt tcagcgggca ggtgagctac tacagtgacg cactcgccgg
    10261 gcgctccacg gcgagtggcg agccgtacca gcctcgcgct ttcacggccg cgcatcgctc
    10321 gttgccgttc ggcaccatcg ttcgcgtgac gagcgacgtt acgggtaaga gtgtttacgt
    10381 gcgcatcaac gaccgcggac cgttcgtgcg cggccgcgtg ctcgacttgt cgcgcgccgc
    10441 cgcggaaagg ctcggtctgc tcggccgcgg cgttctcaaa gtgcgcgcgg agatcgtcga
    10501 gaatgggcct acgaagccgc gcgcgcgccg ccgcacccgc aagcgcaaga gctcgcacta
    10561 gggggtgtcc caagttcctg cccgtcgcga gagccaagaa acgcgagcga gaagcgcgcc
    10621 cgaggagtga gcaccggggg aaggctgaag cctttcgagg agcggagcga cgaggccgtg
    10681 cttcgcagcc gcaggatctt ggctcgcagc agggcgggga acttgggaca ccccctaata
    10741 caatcccgcg ggctcacgct gggagacagc cgtagaaccc accggcaaat ggcggatcgc
    10801 tcgggggtgg gtggaaacac cgaaaagggc gcgcaaatac ggcccctcac ggcgtgcggt
    10861 ccctccgcgc gcttcgttcg tcgacgcagc cgcgcccgcc ccacaccgaa aaaacgcgct
    10921 cacttcgtct cggtcgggcg atcgcgcagg cgatagccaa cacccggttc ggtgacgagc
    10981 cactcgggcc gtgcggggtt tgcctcgatc ttctttcgga gctccgccat gtgaacgcgc
    11041 acgtaatgcg actgctccga ctgattgggc ccccacaccg c
//
LOCUS       DASTKS010001357         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_445408, whole genome shotgun sequence.
ACCESSION   DASTKS010001357 DASTKS010000000
VERSION     DASTKS010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445408"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFQ35_29150"
     CDS             complement(<1..648)
                     /locus_tag="VFQ35_29150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934821.1"
                     /translation="MRLKLSLLLSLSMCVAACGGSSKPAEEPSASSSEEKSAEAGAEK
                     SEGDAPAKGESKEAKKEEPKEESSEPKVSRTAKDVITAPDVLFMFSWNSSDVKEKAEK
                     QCEGEASSNPKKRAACLDREKKKIDFDGMGFKQEKGRWYWLVIKRKGKTLINLHKVPV
                     EFVKDEEHSVVLKPAGKDEGTARGGALTETKVSVPNEYQIEIEDPSLGKVVYEAKI"
     gene            918..>2023
                     /gene="mgtE"
                     /locus_tag="VFQ35_29155"
     CDS             918..>2023
                     /gene="mgtE"
                     /locus_tag="VFQ35_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9934822.1"
                     /translation="MSDDRLTTAELRDAWALLSGAERIEGFHQVDPADADDFFLNLSS
                     REQTQMLLSMPPGERRVWFRMLAPDDAADVVQELPREDRGPWLELLDDTSRGEVTALL
                     AYSEDAAGGLMSPRFARLRPEMTVDEAVRYLRRQARERLETIYYAYVLDQEQRLCGVV
                     SFRDLFAAQPNTLLADVMHTSVVSVADDLDQESVARVMAEHDLNAVPVVDEQGRMKGI
                     VTIDDVVDVVQQAATEDIQKLGGMEALETPYMQTALASLIKKRAGWLSALFIGEMLTA
                     SAMGSYEGEIARAVVLALFVPLIISSGGNSGSQATTLIIRAMALGEIQLKDWWRICRR
                     ELAAGFFLGLILASLGFLRIVVWQAMFHTYGDHYL"
BASE COUNT          340 a          697 c          589 g          397 t
ORIGIN      
        1 gatcttggcc tcgtacacga ccttgccgag tgacggatcc tcaatctcga tttgatactc
       61 gttcggcacc gagaccttgg tctccgtgag cgctccaccg cgcgcggtgc cttcgtcttt
      121 gcccgccggc ttgagcacga cactgtgctc ctcgtccttc acgaactcga cggggacctt
      181 gtgcaggttg atgagggtct tgcccttacg cttgatgacg agccagtacc agcggccctt
      241 ctcctgtttg aaacccatgc cgtcgaagtc gatcttcttc ttctcgcgat cgaggcaggc
      301 ggcacgcttc ttggggttgc tggaggcctc gccttcgcac tgcttctcgg ccttttcctt
      361 cacgtccgag ctgttccaag agaacatgaa cagcacgtcc ggcgccgtga tgacgtcctt
      421 ggccgtacga gagaccttgg gttcggaaga ctcctccttg ggctcttcct tcttcgcttc
      481 cttgctctcg cccttcgcag gagcgtcgcc ctcggacttt tcggcgcccg cctcggcgct
      541 cttttcctcg ctgctcgagg cgctcggttc ctccgcgggc ttcgagctac caccgcaagc
      601 agcgacgcac atcgaaaggc tcaagagcag gctaagtttc aatcgcatgg caccatccca
      661 tttggctcgc gcggcccttc catcgcctct tcggtgaaac ctcgggtaag ctttggggcg
      721 gcgccgcgtc cgacgaaccg tgtcggttac gtagcacggc tctcgggccc gagaaaagta
      781 gaagcccccc ggtgtagctc gaaccgtggc ttcgaaagtt ttcgcgcgtc agcgctttac
      841 gtgccaaaag aagcagagta tcgacccgcc ctgccggcgc cctcgaccgc gaagactgcc
      901 ctttcgatga ggcttccatg tccgatgacc gactcaccac cgcggagctg cgcgacgctt
      961 gggcgctgct ctcgggggcc gagcgcatcg agggatttca tcaggtcgat ccggcggacg
     1021 ccgacgactt cttcctgaac ctgagctcgc gcgagcagac gcaaatgctg ctctcgatgc
     1081 cacccggcga gcgccgagtc tggttccgca tgctcgcccc cgacgacgcc gccgacgtcg
     1141 tccaggagct cccccgcgaa gaccgcggac cttggctcga gttgctcgac gacacgagcc
     1201 gtggagaagt cacggccctg ctcgcgtact cggaggacgc cgccggtggc ttgatgagcc
     1261 cgcggttcgc ccgtttgcgg cccgagatga ccgtcgacga ggcagttcgc tacctacgcc
     1321 ggcaagcgcg cgaacgcctc gaaaccatct actacgccta cgtgctcgat caagagcagc
     1381 gcctttgcgg cgtggtttcg ttccgcgatc tcttcgcggc acagccgaac accctgctcg
     1441 ccgacgtgat gcacacgagc gtggtgagcg tcgccgacga cctcgaccaa gagagcgtcg
     1501 cgcgcgtgat ggccgagcac gacctcaacg cggtgccggt ggtcgacgaa caaggccgga
     1561 tgaaaggtat cgtcaccatc gacgacgtcg tcgacgtcgt gcagcaagcg gccaccgaag
     1621 acatccagaa actcggcggt atggaggccc tcgagacgcc gtacatgcaa acggcgctcg
     1681 cctccctcat caaaaaacgc gccggctggc tctccgccct cttcatcggc gagatgctca
     1741 ccgcgagcgc catgggcagc tacgaaggcg aaatcgcgcg cgcggtcgta ctcgccctct
     1801 tcgtacctct catcatctcg agcggcggca actccggctc ccaagcgacg accctcatca
     1861 tccgcgcgat ggcgctcggt gaaatccagc tgaaagactg gtggcgcatc tgccggcgcg
     1921 agctcgcagc cggcttcttc ttgggactca tcctcgcaag cctcggcttt ctgcgcatcg
     1981 tcgtgtggca ggcgatgttc cacacgtacg gcgaccacta cct
//
LOCUS       DASTKS010001358         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2243
            k141_445660, whole genome shotgun sequence.
ACCESSION   DASTKS010001358 DASTKS010000000
VERSION     DASTKS010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970014
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:08:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,837
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,798
            CDSs (with protein)               :: 5,798
            Genes (RNA)                       :: 28
            tRNAs                             :: 26
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445660"
                     /isolate="SMAG_U2243"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFQ35_29160"
     CDS             complement(<1..725)
                     /locus_tag="VFQ35_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9934823.1"
                     /translation="MRLALVSDIHGNALAFEQVLSHIQRDGVDQIICLGDVVTLGPEP
                     ERTLELLYESRAICILGNHDEFMLRPELVAAYTSIPVLIDAIDWCRSQLSSSSLELIR
                     TFRPRYEQPLNASETLLAFHGTPSSNTTDLLATTEAAIVDEWVGQVPGTVLAGGHTHL
                     QMIRQHRGRLLVNPGSVGMPFKEYVAGAAPEVLSHAEYATIDASGNALKVTLARLALD
                     KPSLRKSVLDVDYPFAAFLSQAYA"
     gene            803..1795
                     /locus_tag="VFQ35_29165"
     CDS             803..1795
                     /locus_tag="VFQ35_29165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET9934824.1"
                     /translation="MSIPSKMCALQQTSLGGPGDLRLVTDAPVPSPGRGEVLVRVDAA
                     GVNFADISRAYGTFRNGPRPPFWAGFEAAGEVVAVGEGVSGPQRGDRVVGVGSGAFAE
                     YAVLYASAALPVPASWTAQQALGLVVNWPTALAAIKPLGRLRPGETVLIHAAAGATGQ
                     AAVTLAKHYGARVIAVASSSKHDAVRALGAEHVLDSRSEDLASQVQRLTAGVGADLVL
                     ESVGGATFKASLSAARRVTGRVVVFGLAGGQSALTNWELVYEQQVQVIGLNIGVLSQA
                     APQIFGELMGELAALIAAEVVTPAHPTAYRLAEGARALLALESRATIGKLALLP"
     gene            1845..2837
                     /locus_tag="VFQ35_29170"
     CDS             1845..2837
                     /locus_tag="VFQ35_29170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET9934825.1"
                     /translation="MVTRAPTKPGEEFAVESRAYRRELLAHCYRMVGSAHDAEDLVQE
                     TYLRAFRSYETFEGKASVRSWLYAIATNVCLTALGSRQMRVLPSGLAGPYEGPGRSAS
                     PVPPGEVSWLEPLPDAWSDATPDDPGTIAIARESLRLALIASLQFLPARQRAVFLLRE
                     VLAFSAEEAAEILGTTVAAIKSGLQRARARLDEVTPAREALLEPTDPRAVELLDGYIA
                     AFERADASLLIDVLRSDAALEATPFRDWVVGRAKCIHLLETYVLGSPGDWRLIATRAN
                     GQPAAVLYRRDAAHLFRGYGVVVLAPTVMGVCRVIAFHEPALVRLFGFQDTLEA"
     gene            complement(2881..3306)
                     /locus_tag="VFQ35_29175"
     CDS             complement(2881..3306)
                     /locus_tag="VFQ35_29175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9934826.1"
                     /translation="MNKRIVIGVFSYLVPTFALGFVWHLVAFKGYYEALAIYRKDVII
                     PFGFLSMSIQGVLFAWVYERLFSERSDLKSRVLGYAAFGAALSWSFTTIAVAAKNIMS
                     SVPGYLLIESGFTLVQWLLVGPLTVLLTRRSGPVRAVHT"
     gene            complement(3338..3784)
                     /locus_tag="VFQ35_29180"
     CDS             complement(3338..3784)
                     /locus_tag="VFQ35_29180"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9934827.1"
                     /translation="MRPNERAEVRRHLAAAPERVFAAFSSAEVVGSWLTPSPEVKLTV
                     LELDFREGGRYRFVYDTPNGERMVVGGVYRKIQAPTRLVFSWLIEPPDIHAGIESQVT
                     VTIAVSGDGSDLVILHERFGRPDANARHAEGWAGALEQLQRLLQRS"
     gene            complement(3784..4110)
                     /locus_tag="VFQ35_29185"
     CDS             complement(3784..4110)
                     /locus_tag="VFQ35_29185"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9934828.1"
                     /translation="MQSAPALDRTFHALSDQTRRSMIHALAGGKARSAGELGKQFNSA
                     QPTISRHLKVLEHANLIERSVEGRVHRFRLRQKPLQEAGEWIARHQRFWNGALDQLET
                     ILGRSE"
BASE COUNT          719 a         1315 c         1395 g          825 t
ORIGIN      
        1 gcgtaagcct gggacaagaa cgccgcgaag gggtagtcga cgtcgagcac cgattttcgc
       61 agactcggct tgtccaatgc caagcgcgcg agcgtcactt tcaacgcatt gccgctcgcg
      121 tcgatggtgg cgtactcggc gtgagacaac acctctggag cggcgcccgc gacgtattcc
      181 ttgaacggca tgccgacact accggggttg accagcaacc taccgcggtg ctggcgtatc
      241 atttgtaaat gggtgtgccc tccggcgagc accgtaccag gaacctggcc gacccactcg
      301 tcgacgattg cagcctcggt cgtcgcgagc aggtccgtcg tgttggagga aggcgtgccg
      361 tggaacgcca ataaggtttc gctcgcgttg agcggctgtt cgtaacgagg gcgaaaggtt
      421 cggatgagct cgagcgagct actgctcaac tgagagcgac accaatcgat cgcgtctatc
      481 aaaaccggaa tgctcgtgta cgctgccacg agctcgggcc gcagcatgaa ctcgtcgtgg
      541 ttgcctaaga tgcagattgc tcggctctcg tataggagct cgagcgtgcg ttcgggttcg
      601 ggcccgagcg tgaccacatc gccaaggcag atgatttggt ccacgccgtc gcgctggatg
      661 tgagagagaa cctgctcgaa agcgagcgcg ttgccgtgga tgtcggacac gagggcgagc
      721 cgcatgcgac gcccagctta gctttccgcg tcgaattcgt cggcgaccga ttctttttgg
      781 cggtgaccga ggtctacacg ccatgagcat tccctcgaaa atgtgtgccc tgcagcagac
      841 ttccttgggc gggcccgggg atctgcgcct cgtcacggat gcgccggtac cgtcgccagg
      901 ccggggcgag gtcctggtcc gagtcgacgc ggccggcgtc aattttgccg atatctcgcg
      961 agcttacggc accttccgga atgggccgcg gcctccgttt tgggctggat tcgaggccgc
     1021 gggcgaggtc gttgcggtgg gtgaaggcgt gagcgggccg caacgggggg accgggtcgt
     1081 cggtgtgggg tcaggtgctt tcgccgaata cgcggtgctt tacgcctccg ccgccctacc
     1141 ggtcccggcg agttggaccg cgcagcaggc gctcggcctg gtcgtgaatt ggccaactgc
     1201 actggccgcg atcaagccac tcggccgcct tcgtccaggg gagaccgtac tcatccacgc
     1261 cgccgcgggc gcgacgggcc aggcagcggt gactctcgcc aagcactacg gtgcgcgagt
     1321 catcgccgtt gcgtcatcga gcaagcacga cgcggtgaga gcgctcggcg ccgaacacgt
     1381 cttggattct cgctccgaag acctcgcttc ccaagtacag aggttgacgg ccggcgtcgg
     1441 tgcggacctc gtgctcgagt ccgttggcgg tgccacgttc aaggccagct taagcgcggc
     1501 gcgacgggtg accggtcgag tcgtcgtctt cggtttggcg ggcggccaaa gcgcgctcac
     1561 taactgggag ctggtgtacg agcagcaggt ccaggtcatc ggcttgaaca tcggggtgtt
     1621 gagccaggcc gcaccgcaga ttttcggaga gctcatgggt gagctcgccg cgttgatcgc
     1681 ggccgaggtg gtcactcctg ctcaccccac cgcttaccgc ttggcggagg gcgcacgggc
     1741 cctcttggct ctggaaagcc gcgcgaccat cggtaagctt gcgctgctcc cttgatgatt
     1801 cagtttgccg tcgcctgctc tcggtcgtcg ctcggacgcg cggtatggtg acgcgcgcgc
     1861 cgacgaaacc tggtgaagag ttcgcggtcg agtctcgcgc gtaccgccgc gagctcttgg
     1921 cccattgcta ccgaatggtc ggctccgctc atgacgcgga ggacttggtg caggaaactt
     1981 atcttcgcgc gtttcgttcc tacgaaacgt tcgagggcaa ggcatccgtg cggtcgtggc
     2041 tgtacgcgat cgccaccaac gtctgcctga ccgcgttggg ctcgcgtcag atgcgtgtct
     2101 tgccttcggg gctcgcgggt ccgtatgagg gtccggggcg ttcggcgtct cccgtgccgc
     2161 cgggcgaagt gtcgtggttg gaaccgctgc ccgatgcctg gtccgacgcc accccagacg
     2221 accccggaac gattgcgatc gcgcgcgaat cgctacggct tgcgctcatt gccagcttgc
     2281 agttcctacc cgctcgtcag cgcgcagtgt tcctgctgcg cgaggtgctc gcattctccg
     2341 cggaagaggc cgccgagatc ctcggaacca cggttgcggc gatcaagagc ggtcttcagc
     2401 gcgctcgtgc ccggctggac gaggtgacgc ccgcacgtga agccctcctc gaaccaaccg
     2461 acccgcgcgc ggtagagtta ctcgacggtt acatcgcggc gttcgagcgc gcggacgcga
     2521 gcttgctgat cgacgtcttg cggtcggacg ctgccctcga ggcgaccccg ttccgcgatt
     2581 gggtggtggg tcgggcgaaa tgcatccatc tgctcgagac atatgtgttg ggctcgcctg
     2641 gcgactggcg actgatcgcg acgcgagcga acggccaacc cgcagcggtc ttgtatcggc
     2701 gagatgcggc ccacctcttc agaggttatg gcgtcgtggt gctcgctcca actgtgatgg
     2761 gcgtctgtcg cgtgattgcg ttccacgagc cggcgctcgt gcgattgttc ggatttcagg
     2821 acacgctcga ggcttgagtg cgtttcgatt gggcctcggt tggggcgttg atgcttcggt
     2881 tcaggtgtgt accgctcgca ccggtcccga gcgtcgcgtc aagagcaccg tcagtgggcc
     2941 caccagcagc cactgcacca gcgtgaagcc ggactcgatc agcaaatagc cgggaaccga
     3001 gctcatgatg ttcttcgcgg cgaccgcaat cgtcgtgaaa ctccacgaca gcgccgcacc
     3061 gaatgcggcg tacccgagaa ctcgcgactt gaggtcggag cgctcggaga agagacgctc
     3121 gtatacccag gcaaacagca ccccttggat ggacatcgat agaaacccga acgggatgat
     3181 gacgtccttc cgatagattg cgagagcctc gtagtagccc ttgaacgcga ccaggtgcca
     3241 cacgaagccg agtgcaaaag tggggactag atacgaaaat acgccgataa cgatgcgttt
     3301 gttcatggtg attgcctcgt cgaagtgagg ggaggtctca cgagcgctgc agtagccgtt
     3361 gcagttgctc gagcgcgccg gcccaccctt ccgcgtgacg cgcgttcgcg tcggggcgcc
     3421 cgaaccgctc atgcaaaatg acgagatcgc tgccatcgcc gcttaccgcg atcgtcaccg
     3481 tcacttgtga ctcgatccca gcgtgaatgt ccggcggctc gatcaaccac gagaagacca
     3541 gccgagtcgg cgcttggatt ttgcgataaa cgccaccgac caccatgcgc tctccgttcg
     3601 gagtgtcgta aacgaatcgg tagcgaccgc cttccctgaa gtcgagctcg agcacggtga
     3661 gcttcacctc cggtgagggc gtgagccagc tcccaacgac ttcggcgctc gaaaacgctg
     3721 cgaacactcg ctcgggcgcg gcggccagat ggcggcgcac ctctgctcgt tcgttcggcc
     3781 tcattactcg ctccggccga gaatcgtttc gagttgatcg agcgcgccgt tccagaagcg
     3841 ctgatgcctt gcgatccact cgccggcttc ttgcaacggc ttttggcgaa gacgaaaccg
     3901 gtgcacacgc ccctcgacgc tgcgctcgat gaggttcgca tgctcgagca ctttcagatg
     3961 gcgcgagatg gtcggttggg ccgagttgaa ctgcttaccg agctcaccgg cgctccgtgc
     4021 cttgccaccg gccagcgcgt ggatcatcga tcggcgcgtt tgatccgaaa gggcgtgaaa
     4081 cgtgcgatcg agcgcggggg cagattgcat agccgattgg ctatatgttt tggtgagccg
     4141 cgtcaagccc cgaattcacc gaggccgctc cttggctagg gtcgtcgcgc ctgctctcag
     4201 ctcgaaatga cgcgcaactc gatggtgacg gtatcgccgt cgcctttgtc tccg
//
